{"id": "PMID:1072874", "title": "Adjuvant chemotherapy associated with surgery in the treatment of osteosarcoma. Preliminary results.", "content": "The results are presented of thirty five cases of osteosarcoma in which radical surgery was associated with cyclic chemotherapy with vincrystin, adriamycin and methotrexate. Nine patients had pulmonary metastases at intervals of from three to twenty one months after operation; of these, seven died between six and twenty six months after operation. The remaining twenty six patients are in good health and show no signs of disease at follow up varying from twelve to forty eight months. The mortality rate and the percentage of patients with pulmonary metastase from six to forty eight months after operation are notably lower than in a previous series treated by surgery only.", "contents": "Adjuvant chemotherapy associated with surgery in the treatment of osteosarcoma. Preliminary results. The results are presented of thirty five cases of osteosarcoma in which radical surgery was associated with cyclic chemotherapy with vincrystin, adriamycin and methotrexate. Nine patients had pulmonary metastases at intervals of from three to twenty one months after operation; of these, seven died between six and twenty six months after operation. The remaining twenty six patients are in good health and show no signs of disease at follow up varying from twelve to forty eight months. The mortality rate and the percentage of patients with pulmonary metastase from six to forty eight months after operation are notably lower than in a previous series treated by surgery only."}
{"id": "PMID:1072875", "title": "A study of the development of fracture callus in the presence of an experimentally induced osteosarcoma.", "content": "The results of two experiments are reported in which the formation of an osteosarcoma was induced in mice by the intraosseous injection of Moloney's virus. In the first group of fifty mice, a complete diaphyseal fracture was carried out nine days later at the site of the tumour. In the second group of 200 mice, a partial fracture was produced at the time of injection so that immobilisation was assured. The effects of cyclophosphamide and calcitonin administration were also studied in this group. The course of the repair processes of the bone was studied in both groups, and showed that, even in the presence of an osteosarcoma, these begin and can reach completion, though obstructed and delayed by the tumour.", "contents": "A study of the development of fracture callus in the presence of an experimentally induced osteosarcoma. The results of two experiments are reported in which the formation of an osteosarcoma was induced in mice by the intraosseous injection of Moloney's virus. In the first group of fifty mice, a complete diaphyseal fracture was carried out nine days later at the site of the tumour. In the second group of 200 mice, a partial fracture was produced at the time of injection so that immobilisation was assured. The effects of cyclophosphamide and calcitonin administration were also studied in this group. The course of the repair processes of the bone was studied in both groups, and showed that, even in the presence of an osteosarcoma, these begin and can reach completion, though obstructed and delayed by the tumour."}
{"id": "PMID:1072935", "title": "Background to the therapeutic potential of essential amino acid analogues.", "content": "Experimental investigation of the metabolic effects of essential amino acid analogues has to be critically assessed against a background of limited experimental techniques and very incomplete understanding of both protein metabolism and protein requirements. Although interest in the therapeutic use of these compounds initially centered on patients with chronic renal failure, their therapeutic potential is by no means confined to renal disease. The fact that metabolic effects of essential amino acid analogues cannot be explained simply in terms of their conversion to the essential amino acids raises the possibility that they may prove to be useful inducers of protein anabolism. Improvement in nitrogen balance may only be of the order of a few grams and measurable nitrogen balance is only a small proportion of total protein turnover, nevertheless, small changes may prove to be of considerable clinical significance.", "contents": "Background to the therapeutic potential of essential amino acid analogues. Experimental investigation of the metabolic effects of essential amino acid analogues has to be critically assessed against a background of limited experimental techniques and very incomplete understanding of both protein metabolism and protein requirements. Although interest in the therapeutic use of these compounds initially centered on patients with chronic renal failure, their therapeutic potential is by no means confined to renal disease. The fact that metabolic effects of essential amino acid analogues cannot be explained simply in terms of their conversion to the essential amino acids raises the possibility that they may prove to be useful inducers of protein anabolism. Improvement in nitrogen balance may only be of the order of a few grams and measurable nitrogen balance is only a small proportion of total protein turnover, nevertheless, small changes may prove to be of considerable clinical significance."}
{"id": "PMID:1072939", "title": "Evidence for an anabolic action of essential amino acid analogues in uremia and starvation.", "content": "Nitrogen-free analogues of essential amino acids, when administered with those essential amino acids for which analogues are ineffective or unavailable, exert three actions that may be beneficial in protein-deficient or protein-intolerant subjects. First, they bring about an increase in the concentrations of essential amino acids in the blood at the expense of the concentrations of certain non-essential amino acids, notably alanine and glutamine. This effect is most readily demonstrated in children with congenital defects of the urea cycle enzymes, but can also be seen during daily therapy of adults with portal-systemic encephalopathy. Second, these compounds promote nitrogen balance through their suppressive effect on urea synthesis (an effect not attributable to re-utilization of ammonia derived from urease action in the gut). This action is demonstrable in obese subjects who are already conserving nitrogen maximally at the end of a prolonged fast and can also be shown in the first week of fasting when the branched-chain keto acids alone are administered. In both situations, improved nitrogen conservation persists long after the analogues are metabolized, suggesting enzyme adaptations. In chronic uremics, nitrogen balance can be maintained in some (but not all) patients on very low nitrogen intakes. Third, these mixtures may delay or reverse the progressive decline in glomerular filtration rate characteristic of chronic renal failure in some cases: thus, for example, 5 of 6 patients taken off chronic dialysis have maintained lower serum urea concentrations without evidence of protein malnutrition for periods of 2-24 months.", "contents": "Evidence for an anabolic action of essential amino acid analogues in uremia and starvation. Nitrogen-free analogues of essential amino acids, when administered with those essential amino acids for which analogues are ineffective or unavailable, exert three actions that may be beneficial in protein-deficient or protein-intolerant subjects. First, they bring about an increase in the concentrations of essential amino acids in the blood at the expense of the concentrations of certain non-essential amino acids, notably alanine and glutamine. This effect is most readily demonstrated in children with congenital defects of the urea cycle enzymes, but can also be seen during daily therapy of adults with portal-systemic encephalopathy. Second, these compounds promote nitrogen balance through their suppressive effect on urea synthesis (an effect not attributable to re-utilization of ammonia derived from urease action in the gut). This action is demonstrable in obese subjects who are already conserving nitrogen maximally at the end of a prolonged fast and can also be shown in the first week of fasting when the branched-chain keto acids alone are administered. In both situations, improved nitrogen conservation persists long after the analogues are metabolized, suggesting enzyme adaptations. In chronic uremics, nitrogen balance can be maintained in some (but not all) patients on very low nitrogen intakes. Third, these mixtures may delay or reverse the progressive decline in glomerular filtration rate characteristic of chronic renal failure in some cases: thus, for example, 5 of 6 patients taken off chronic dialysis have maintained lower serum urea concentrations without evidence of protein malnutrition for periods of 2-24 months."}
{"id": "PMID:1073183", "title": "Lead insult to the dentition in the leopard.", "content": "Fracture of developing permanent canine teeth occurred in a black leopard hospitalized for lead poisoning. Spectrographic identification of lead in soft tissue at the site of injury suggests a relationship between lead insult and the developing dentition.", "contents": "Lead insult to the dentition in the leopard. Fracture of developing permanent canine teeth occurred in a black leopard hospitalized for lead poisoning. Spectrographic identification of lead in soft tissue at the site of injury suggests a relationship between lead insult and the developing dentition."}
{"id": "PMID:1073184", "title": "A chemical evaluation of collagen and hydroxyproline after treatment with GK-101 (N-Chloroglycine).", "content": "The reactions of GK-101 (N-chloroglycine) with collagen and hydroxyproline, were studied with regard to caries removal. After reaction with N-chloroglycine, collagen was analyzed for bound available chlorine by iodiometric titration, and hydroxyproline conversion to pyrrole-2-carboxylic acid was measured by they production of a red chromogen after PAB treatment. The results indicated that collagen was chlorinated by N-chloroglycine and hydroxyproline was converted to pyrrole-2-carboxylic acid after N-chloroglycine reaction. These findings tend to indicate two possible pathways of N-chloroglycine protein interaction.", "contents": "A chemical evaluation of collagen and hydroxyproline after treatment with GK-101 (N-Chloroglycine). The reactions of GK-101 (N-chloroglycine) with collagen and hydroxyproline, were studied with regard to caries removal. After reaction with N-chloroglycine, collagen was analyzed for bound available chlorine by iodiometric titration, and hydroxyproline conversion to pyrrole-2-carboxylic acid was measured by they production of a red chromogen after PAB treatment. The results indicated that collagen was chlorinated by N-chloroglycine and hydroxyproline was converted to pyrrole-2-carboxylic acid after N-chloroglycine reaction. These findings tend to indicate two possible pathways of N-chloroglycine protein interaction."}
{"id": "PMID:1073187", "title": "Longitudinal effects of an antibacterial oral rinse on gingivitis and dental plaque.", "content": "The purpose of this study was to determine what effect oral rinse containing alexidine dihydrochloride (Calgon Consumer Products Company, a Subsidiary of Merck & Co.) would have on gingivitis and plaque. Following a dental prophylaxis, 167 male and female, 7-12-year-old students in Puerto Rican elementary school rinsed twice daily for 60 sec. with 10 ml of either a placebo oral rinse or one containing alexidine dihydrochloride. All students brushed their teeth daily in school. Gingivitis and plaque were scored on all buccal and lingual tooth surfaces prior to the prophylaxis and on days 30, 90, and 160. On these dates, blood and urine analysis were also made. A second prophylaxis was given at the end of the study. The adjusted mean gingivitis and plaque scores were compared between the treatment and placebo groups at 30, 90, and 160 days using analysis of covariance. The alexidine dihydrochloride oral rinse was found to reduce significantly gingivitis after 160 days (p less than 0.02) of treatment and plaque scores 30 days (p less than 0.01), 90 days (p less than 0.001), and 160 days (p less than 0.001) after treatment. No blood or urine changes were attributed to the active ingredient oral rinse.", "contents": "Longitudinal effects of an antibacterial oral rinse on gingivitis and dental plaque. The purpose of this study was to determine what effect oral rinse containing alexidine dihydrochloride (Calgon Consumer Products Company, a Subsidiary of Merck & Co.) would have on gingivitis and plaque. Following a dental prophylaxis, 167 male and female, 7-12-year-old students in Puerto Rican elementary school rinsed twice daily for 60 sec. with 10 ml of either a placebo oral rinse or one containing alexidine dihydrochloride. All students brushed their teeth daily in school. Gingivitis and plaque were scored on all buccal and lingual tooth surfaces prior to the prophylaxis and on days 30, 90, and 160. On these dates, blood and urine analysis were also made. A second prophylaxis was given at the end of the study. The adjusted mean gingivitis and plaque scores were compared between the treatment and placebo groups at 30, 90, and 160 days using analysis of covariance. The alexidine dihydrochloride oral rinse was found to reduce significantly gingivitis after 160 days (p less than 0.02) of treatment and plaque scores 30 days (p less than 0.01), 90 days (p less than 0.001), and 160 days (p less than 0.001) after treatment. No blood or urine changes were attributed to the active ingredient oral rinse."}
{"id": "PMID:1073188", "title": "The effect of local anesthetic solutions on the blood vessels of the rat molars.", "content": "The effect of solutions of lidocaine, with and without adrenaline, on the blood vessels of the dental pulp and periodontal ligament in rat molars was examined. The vessels were visualized by intravascular injection of a supension of carbon particles. The results indicate that the anesthetic solution alone does have a local effect on the blood vessels. Local anesthetic solutions containing adrenaline had a more marked effect; in addition, a dose-dependent systemic effect of adrenaline was noted.", "contents": "The effect of local anesthetic solutions on the blood vessels of the rat molars. The effect of solutions of lidocaine, with and without adrenaline, on the blood vessels of the dental pulp and periodontal ligament in rat molars was examined. The vessels were visualized by intravascular injection of a supension of carbon particles. The results indicate that the anesthetic solution alone does have a local effect on the blood vessels. Local anesthetic solutions containing adrenaline had a more marked effect; in addition, a dose-dependent systemic effect of adrenaline was noted."}
{"id": "PMID:1073189", "title": "Enhanced fluoride uptake by teeth through use of iontophoretic techniques.", "content": "The effects of electric currents on the uptake of fluoride ions by human teeth, in vitro were determined by measuring the change in fluoride ion concentration of the electrolyte in which teeth were subjected to the electrochemical treatment. There was an enhanced uptake when the gold electrode embedded in the tooth was polarized anodically. Evidence that the fluoride ions were taken upon by the teeth was shown by subsequent cathodic polarization of the gold electrode in a fluoride free electrolyte and by measurement of fluoride release into the solution.", "contents": "Enhanced fluoride uptake by teeth through use of iontophoretic techniques. The effects of electric currents on the uptake of fluoride ions by human teeth, in vitro were determined by measuring the change in fluoride ion concentration of the electrolyte in which teeth were subjected to the electrochemical treatment. There was an enhanced uptake when the gold electrode embedded in the tooth was polarized anodically. Evidence that the fluoride ions were taken upon by the teeth was shown by subsequent cathodic polarization of the gold electrode in a fluoride free electrolyte and by measurement of fluoride release into the solution."}
{"id": "PMID:1073205", "title": "The use of aneuploids in studies of genetics, breeding, and evolution in wheat.", "content": "In wheat a unique series of aneuploids is available, ranging from all 21 possible monosomics to complex types which are simultaneously deficient for one chromosome and duplicate for another. Furthermore, lines with chromosomes from related, alien species either added to or substituted for wheat chromosomes are in common cytological use. This contribution considers the use of this range of material in studies designed to elucidate the evolutionary relationships of the species, investigations of the genetics of a polyploid with cytological diploidization, and in potential breeding manipulations.", "contents": "The use of aneuploids in studies of genetics, breeding, and evolution in wheat. In wheat a unique series of aneuploids is available, ranging from all 21 possible monosomics to complex types which are simultaneously deficient for one chromosome and duplicate for another. Furthermore, lines with chromosomes from related, alien species either added to or substituted for wheat chromosomes are in common cytological use. This contribution considers the use of this range of material in studies designed to elucidate the evolutionary relationships of the species, investigations of the genetics of a polyploid with cytological diploidization, and in potential breeding manipulations."}
{"id": "PMID:1073206", "title": "Prospects and perspectives in mutation breeding.", "content": "Induction of mutations, primarily a method of generating variation, can contribute to plant improvement when combined with selection, or recombination and selection, or with other methods of manipulating genetic variation. As a source of variability, induced mutations supplement naturally occurring variation. When specific mutants are selected following mutagenic treatments it is highly likely that a number of mutational changes will have occurred in the selected genotype. Hence, although most of the mutant varieties released so far have resulted from mutation and direct selection, the future trend will be for increasing use of mutants in association with recombination. Whereas induced mutations are generally regarded as random events, there are suggestions of some mutational specificity in response to different mutagenic agents and treatments. The best immediate prospects for increasing specificity lie in the manipulation of the selection environment. Biochemical selection applied to large number of plant cells in culture to locate mutations in specific biosynthetic pathways and the subsequent regeneration of whole plants offers great prospect for reducing the cost of breeding programs and altering the amount or composition of a desired end or intermediate product. Mutations in conbination with other techniques of genetic engineering will constitute the tools of the plant breeders of the future. Their present role in plant breeding has been established. They have advantages in certain situations, disadvantages in others. Greater understanding will lead to their more widespread use.", "contents": "Prospects and perspectives in mutation breeding. Induction of mutations, primarily a method of generating variation, can contribute to plant improvement when combined with selection, or recombination and selection, or with other methods of manipulating genetic variation. As a source of variability, induced mutations supplement naturally occurring variation. When specific mutants are selected following mutagenic treatments it is highly likely that a number of mutational changes will have occurred in the selected genotype. Hence, although most of the mutant varieties released so far have resulted from mutation and direct selection, the future trend will be for increasing use of mutants in association with recombination. Whereas induced mutations are generally regarded as random events, there are suggestions of some mutational specificity in response to different mutagenic agents and treatments. The best immediate prospects for increasing specificity lie in the manipulation of the selection environment. Biochemical selection applied to large number of plant cells in culture to locate mutations in specific biosynthetic pathways and the subsequent regeneration of whole plants offers great prospect for reducing the cost of breeding programs and altering the amount or composition of a desired end or intermediate product. Mutations in conbination with other techniques of genetic engineering will constitute the tools of the plant breeders of the future. Their present role in plant breeding has been established. They have advantages in certain situations, disadvantages in others. Greater understanding will lead to their more widespread use."}
{"id": "PMID:1073207", "title": "Artificial mutagenesis as an aid in overcoming genetic vulnerability of crop plants.", "content": "Artificially induced genetic variation is being used effectively to supplement or complement sources of natural origin for practical plant breeding. Thus, creating genetic variation uill become increasingly important as crop genetic resources become more difficult to obtain via plant exploration. The aritificial induction of useful genetic variation offers important elements that can be used for overcoming genetic vulnerability: (1) new, previously unknown alleles can be induced in crop plant species to broaden the base of variation; (2) useful genetic variation can be induced in modern cultivars helping to shorten breeding time or to extend production \"life\"; (3) characteristics of existing genetic resource stocks can be improved to make them more useful in breeding; and (4) recombination in crosses may be enhanced. The performance of induced mutant crop cultivars and the successful uses of induced genetic variation in cross breeding indicate that artificial mutagenesis will play an increasingly greater role in plant breeding.", "contents": "Artificial mutagenesis as an aid in overcoming genetic vulnerability of crop plants. Artificially induced genetic variation is being used effectively to supplement or complement sources of natural origin for practical plant breeding. Thus, creating genetic variation uill become increasingly important as crop genetic resources become more difficult to obtain via plant exploration. The aritificial induction of useful genetic variation offers important elements that can be used for overcoming genetic vulnerability: (1) new, previously unknown alleles can be induced in crop plant species to broaden the base of variation; (2) useful genetic variation can be induced in modern cultivars helping to shorten breeding time or to extend production \"life\"; (3) characteristics of existing genetic resource stocks can be improved to make them more useful in breeding; and (4) recombination in crosses may be enhanced. The performance of induced mutant crop cultivars and the successful uses of induced genetic variation in cross breeding indicate that artificial mutagenesis will play an increasingly greater role in plant breeding."}
{"id": "PMID:1073210", "title": "Prospects of breeding for salt tolerance in rice.", "content": "Variations with regard to salt tolerance were observed in rice varieties, Blue bonnet, IR-8, Jhona-349, and Magnolia. Crosses (F1) between relatively salt-resistant (Jhona-349) and salt-sensitive (Magnolia) varieties were highly resistant to salinity. The F2 population resulted in some salt-resistant combinations. In the F3 and F4 populations, some desirable salt-resistant progenies were selected. Results obtained suggest that selection for salt tolerance may be possible within hybrid populations.", "contents": "Prospects of breeding for salt tolerance in rice. Variations with regard to salt tolerance were observed in rice varieties, Blue bonnet, IR-8, Jhona-349, and Magnolia. Crosses (F1) between relatively salt-resistant (Jhona-349) and salt-sensitive (Magnolia) varieties were highly resistant to salinity. The F2 population resulted in some salt-resistant combinations. In the F3 and F4 populations, some desirable salt-resistant progenies were selected. Results obtained suggest that selection for salt tolerance may be possible within hybrid populations."}
{"id": "PMID:1073215", "title": "Useful genetic sources of economic importance and their utlization in wheat-breeding programs in Pakistan.", "content": "Wheat breeders the world over have been utilizing genetic sources to tailor the varieties to meet ever-changing requirements. In the late 1940s Dr. Borlaug at CIMMYT recognized that further increase in yield would be possible only if lodging in the existing wheat varieties could be avoided, for which he began to look for a suitable source for dwarfness. The Japanese had developed semidwarf Norin strains through a series of crosses involving a local line, Daruma; American soft red winter variety, Fultz; and American hard red winter variety, Turkey Red. One of the Norin strains, Norin-10, was used in the breeding programs, first in Italy and then in the United States where Dr. Orville Vogel developed two to three semidwarf varieties. In 1953 Dr. Vogel supplied some F2 seeds of Norin-10 Brevor to the CIMMYT program in Mexico, where this source was employed extensively in the breeding rogram; a large number of varieties were developed, some of which worth mentioning are Pitic, Penjamo, Lerma, Sonora, Inia, Tobari, and Siete Cerros.", "contents": "Useful genetic sources of economic importance and their utlization in wheat-breeding programs in Pakistan. Wheat breeders the world over have been utilizing genetic sources to tailor the varieties to meet ever-changing requirements. In the late 1940s Dr. Borlaug at CIMMYT recognized that further increase in yield would be possible only if lodging in the existing wheat varieties could be avoided, for which he began to look for a suitable source for dwarfness. The Japanese had developed semidwarf Norin strains through a series of crosses involving a local line, Daruma; American soft red winter variety, Fultz; and American hard red winter variety, Turkey Red. One of the Norin strains, Norin-10, was used in the breeding programs, first in Italy and then in the United States where Dr. Orville Vogel developed two to three semidwarf varieties. In 1953 Dr. Vogel supplied some F2 seeds of Norin-10 Brevor to the CIMMYT program in Mexico, where this source was employed extensively in the breeding rogram; a large number of varieties were developed, some of which worth mentioning are Pitic, Penjamo, Lerma, Sonora, Inia, Tobari, and Siete Cerros."}
{"id": "PMID:1073216", "title": "Natural and induced genetic variability in wheat.", "content": "An account is given of the genetic variability in primitive wheats collected from the northwest Pakistan. Apart from genetic sources for disease resistance, drought tolerance, and other plant characters, new sources of possible height reduction in wheat were identified and described. The taller-growing primitive wheats have better emergence, perhaps largely because of their longer coleoptiles. A detailed account is given of comparative genetic studies of height-reducing genes, through both conventional genetic analysis techniques and induced mutation breeding procedures. Both approaches demonstrated the effect of height genes on grain size. At the present time there is a linear relationship between plant height and 1000-kernel weight. Possibilities of achieving a break-through in wheat yields through increased kernel size in short wheats are discussed.", "contents": "Natural and induced genetic variability in wheat. An account is given of the genetic variability in primitive wheats collected from the northwest Pakistan. Apart from genetic sources for disease resistance, drought tolerance, and other plant characters, new sources of possible height reduction in wheat were identified and described. The taller-growing primitive wheats have better emergence, perhaps largely because of their longer coleoptiles. A detailed account is given of comparative genetic studies of height-reducing genes, through both conventional genetic analysis techniques and induced mutation breeding procedures. Both approaches demonstrated the effect of height genes on grain size. At the present time there is a linear relationship between plant height and 1000-kernel weight. Possibilities of achieving a break-through in wheat yields through increased kernel size in short wheats are discussed."}
{"id": "PMID:1073214", "title": "Tissue culture studies on Amaranthus viridis.", "content": "Amaranthus viridis is a plant that is very widely distributed in our country, grows easily, and is eaten by low-income groups. It has been found that the proteins of this plant, like most other plants, are deficient in some essential amino acids. We were able to develop cultures from the cut-end parts of this plant tissue. The commonly used media were modified to achieve good and fast growth. Results have been obtained on the techniques, histology, chromosomal, and biochemical studies which indicate that the plant could be useful as a research tool to study problems in crop improvement at the cellular level.", "contents": "Tissue culture studies on Amaranthus viridis. Amaranthus viridis is a plant that is very widely distributed in our country, grows easily, and is eaten by low-income groups. It has been found that the proteins of this plant, like most other plants, are deficient in some essential amino acids. We were able to develop cultures from the cut-end parts of this plant tissue. The commonly used media were modified to achieve good and fast growth. Results have been obtained on the techniques, histology, chromosomal, and biochemical studies which indicate that the plant could be useful as a research tool to study problems in crop improvement at the cellular level."}
{"id": "PMID:1073218", "title": "Synthetic amphiploids in breeding--genetic and evolutionary studies in wheat.", "content": "Synthetic amphiploids play an important role in breeding programs of wheat and in the genetic and evolutionary studies of the wheat group. One of the most obvious uses of amphiploidy has been the development of hexaploid and octaploid Triticale. The utilization of synthetic amphiploids as a means of introducing commercially important features of related species into cultivated wheat is now well documented. The projects to produce hybrid wheat are based on the manipulation of cytoplasms and restorers by means of artificially obtained amphiploids. The amphiploidization of interspecific hybrids by treatment with colchicine results in homozygosity. Therefore, a direct use of synthetic amphiploids for genetic studies has been limited to some extent. More often they were used indirectly, viz. as the initial step in the production of single chromosome addition and substitution lines. Such lines were used for detailed and highly informative genetic analyses and also as sources of alien variation for wheat breeding. Crosses between some of the synthetic amphiploids resembling natural species and the cultivated wheats have been successfully used in the evolutionary studies of wheats.", "contents": "Synthetic amphiploids in breeding--genetic and evolutionary studies in wheat. Synthetic amphiploids play an important role in breeding programs of wheat and in the genetic and evolutionary studies of the wheat group. One of the most obvious uses of amphiploidy has been the development of hexaploid and octaploid Triticale. The utilization of synthetic amphiploids as a means of introducing commercially important features of related species into cultivated wheat is now well documented. The projects to produce hybrid wheat are based on the manipulation of cytoplasms and restorers by means of artificially obtained amphiploids. The amphiploidization of interspecific hybrids by treatment with colchicine results in homozygosity. Therefore, a direct use of synthetic amphiploids for genetic studies has been limited to some extent. More often they were used indirectly, viz. as the initial step in the production of single chromosome addition and substitution lines. Such lines were used for detailed and highly informative genetic analyses and also as sources of alien variation for wheat breeding. Crosses between some of the synthetic amphiploids resembling natural species and the cultivated wheats have been successfully used in the evolutionary studies of wheats."}
{"id": "PMID:1074331", "title": "[Pathohistological study on the effect of various methods of osteosynthesis on the osteogenesis in experimental fracture of the lower jaw].", "content": "The lower jaw of 60 dogs was fractured and effects of wire and three variants of chemical osteosynthesis have been followed up concerning validity, course and rapidity of osteogenesis. A wire of 0.4 mm in the diameter was applied in wire osteosynthesis and autopolymerizing acryalte (\"Simplex\" Galenica) in chemical osteosynthesis with briding of the fracture line interfragmentarily or mixed with bone scrapings. The pathohistological examinations performed 7, 14, 21, 60 and 90 days after the intervention and osteosynthesis employment have shown that there is no difference in validity, course and rapidity of osteogenesis in fractures of the lower jaw under conditions of wire and pure chemical osteosynthesis. Since there is no histochemical obstacles and taking into account advantages of chemical over wire osteosynthesis it should be recommended in fractures of the lower jaw.", "contents": "[Pathohistological study on the effect of various methods of osteosynthesis on the osteogenesis in experimental fracture of the lower jaw]. The lower jaw of 60 dogs was fractured and effects of wire and three variants of chemical osteosynthesis have been followed up concerning validity, course and rapidity of osteogenesis. A wire of 0.4 mm in the diameter was applied in wire osteosynthesis and autopolymerizing acryalte (\"Simplex\" Galenica) in chemical osteosynthesis with briding of the fracture line interfragmentarily or mixed with bone scrapings. The pathohistological examinations performed 7, 14, 21, 60 and 90 days after the intervention and osteosynthesis employment have shown that there is no difference in validity, course and rapidity of osteogenesis in fractures of the lower jaw under conditions of wire and pure chemical osteosynthesis. Since there is no histochemical obstacles and taking into account advantages of chemical over wire osteosynthesis it should be recommended in fractures of the lower jaw."}
{"id": "PMID:1074332", "title": "[Proximal gastric vagotomy without drainage in the treatment of duodenal ulcer].", "content": "Observations in the treatment of 107 patients with duodenal ulcer by proximal gastric vagotomy without drainage are reported. Operative technique has been described. There were no operative mortality and immediate postoperative complications rarely occured. Incomplete vagotomy was proved in 2,8 of the operated patients. It has been concluded that the best results could be expected insimple duodenal ulcer, some forms of duodenal ulcer bleeding, perforation of acute ulcer and erosive gastritis. Additional stenosis was observed in some penetrating duodenal ulcers. Optimism is justified in a long-term prognosis. Recurrent ulcer was proved in 2 patients.", "contents": "[Proximal gastric vagotomy without drainage in the treatment of duodenal ulcer]. Observations in the treatment of 107 patients with duodenal ulcer by proximal gastric vagotomy without drainage are reported. Operative technique has been described. There were no operative mortality and immediate postoperative complications rarely occured. Incomplete vagotomy was proved in 2,8 of the operated patients. It has been concluded that the best results could be expected insimple duodenal ulcer, some forms of duodenal ulcer bleeding, perforation of acute ulcer and erosive gastritis. Additional stenosis was observed in some penetrating duodenal ulcers. Optimism is justified in a long-term prognosis. Recurrent ulcer was proved in 2 patients."}
{"id": "PMID:1074333", "title": "[Obstructing lesions of the trachea following tracheotomy].", "content": "Within the last four years 8 patients with tracheal stenosis were treated at the ORL Clinic of the M.M.A. The sites of stenosis were at the site of tracheostome, anterior and lateral tracheal walls and circular stenosis of the whole tracheal lumen. Two patients had combined stenosis of trachea and tracheostome. The authors have applied combined palliative surgical interventions without radical approaches but with the employment of longer passive dilatation. Six patients were cured, the complete decannilman was performed, one patient quitted the treatment while one is still under treatment.", "contents": "[Obstructing lesions of the trachea following tracheotomy]. Within the last four years 8 patients with tracheal stenosis were treated at the ORL Clinic of the M.M.A. The sites of stenosis were at the site of tracheostome, anterior and lateral tracheal walls and circular stenosis of the whole tracheal lumen. Two patients had combined stenosis of trachea and tracheostome. The authors have applied combined palliative surgical interventions without radical approaches but with the employment of longer passive dilatation. Six patients were cured, the complete decannilman was performed, one patient quitted the treatment while one is still under treatment."}
{"id": "PMID:1074340", "title": "[Stomatologic preparation of patients with malignancies of the maxillofacial region for radiation therapy].", "content": "A series of 3456 patients with malignant tumours of the maxillofacial region have been treated at the Clinic for Maxillofacial and Oral Surgery of the Military Medical Academy, Stomatological Clinic of the M.M.A. and Radiological Institute of Medical Faculty in Belgrade. The most frequent localization was lips and mucosa of the oral cavity, 77,9%. The irradiation therapy was applied in 42,3% of the patients and combined surgery and irradiation therapy in 28,6% Postirradiation changes of the oral cavity mucosa were found in 25% of the patients, teeth changes in 50% and Candida albicans was found in 56% of the patients. In order to prevent irradiation and postirradiation complications, all the patients with malignant tumours foressen for irradiation therapy should undergo a detailed stomatological examination and sanation of the oral cavity and teeth. The authors advocate team work in establishing the diagnosis and treating in which an important place should have stomatologist.", "contents": "[Stomatologic preparation of patients with malignancies of the maxillofacial region for radiation therapy]. A series of 3456 patients with malignant tumours of the maxillofacial region have been treated at the Clinic for Maxillofacial and Oral Surgery of the Military Medical Academy, Stomatological Clinic of the M.M.A. and Radiological Institute of Medical Faculty in Belgrade. The most frequent localization was lips and mucosa of the oral cavity, 77,9%. The irradiation therapy was applied in 42,3% of the patients and combined surgery and irradiation therapy in 28,6% Postirradiation changes of the oral cavity mucosa were found in 25% of the patients, teeth changes in 50% and Candida albicans was found in 56% of the patients. In order to prevent irradiation and postirradiation complications, all the patients with malignant tumours foressen for irradiation therapy should undergo a detailed stomatological examination and sanation of the oral cavity and teeth. The authors advocate team work in establishing the diagnosis and treating in which an important place should have stomatologist."}
{"id": "PMID:1074344", "title": "Acquired erythroenzymopathies in blood disorders: study of 200 cases.", "content": "Enzyme abnormalities are frequently found in the red cells of patients with various acquired blood disorders. In leukaemias, preleukaemic states and bone marrow insufficiencies with or without sideroblastosis, changes in enzyme activity are usually characterized by the coexistence of deficiency of some enzymes and an increased activity of others. The most frequently decreased activities are those of pyruvate kinase, phosphofructokinase,2,3-diphosphoglycerate mutase and adenylate kinase; the most frequently increased activities are those of hexokinase, aldolase, enolase, 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase. In primary myelofibrosis and in polycythaemia rubra vera, enzyme deficiencies are infrequent and differ from those observed in leukaemias and related disorders. Phosphohexose isomerase and phosphoglucomutase deficiencies seem relatively specific for polycythaemia rubra vera. Explanations for the acquired enzymopathies are still at the stage of hypothesis. The theory of multiple genetic damage may explain some findings but has not yet been proved right. The possibility of post-translational molecular modification is suggested as a working hypothesis.", "contents": "Acquired erythroenzymopathies in blood disorders: study of 200 cases. Enzyme abnormalities are frequently found in the red cells of patients with various acquired blood disorders. In leukaemias, preleukaemic states and bone marrow insufficiencies with or without sideroblastosis, changes in enzyme activity are usually characterized by the coexistence of deficiency of some enzymes and an increased activity of others. The most frequently decreased activities are those of pyruvate kinase, phosphofructokinase,2,3-diphosphoglycerate mutase and adenylate kinase; the most frequently increased activities are those of hexokinase, aldolase, enolase, 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase. In primary myelofibrosis and in polycythaemia rubra vera, enzyme deficiencies are infrequent and differ from those observed in leukaemias and related disorders. Phosphohexose isomerase and phosphoglucomutase deficiencies seem relatively specific for polycythaemia rubra vera. Explanations for the acquired enzymopathies are still at the stage of hypothesis. The theory of multiple genetic damage may explain some findings but has not yet been proved right. The possibility of post-translational molecular modification is suggested as a working hypothesis."}
{"id": "PMID:1074343", "title": "Percutaneous cervical cordotomy.", "content": "Percutaneous cervical cordotomy provides a nonoperative opportunity to interrupt the lateral spinothalamic pathways in patients with pain who are not good operative risks for an open cordotomy. The technique is relatively simple, but requires some practice. Indications should be the same as for open cordotomy, with critical patient selection. Results are comparable to those of surgical cordotomy.", "contents": "Percutaneous cervical cordotomy. Percutaneous cervical cordotomy provides a nonoperative opportunity to interrupt the lateral spinothalamic pathways in patients with pain who are not good operative risks for an open cordotomy. The technique is relatively simple, but requires some practice. Indications should be the same as for open cordotomy, with critical patient selection. Results are comparable to those of surgical cordotomy."}
{"id": "PMID:1075516", "title": "[Retention status of crown/bridge constructions].", "content": "An apparatus consisting of an acid resistant steel cone and a cap of the same maternal, was used for retention and rotation experiments after cementation of the cap on the cone. The upper diameter of the cone was 6 mm, the lower diameter 7 mm and the height 6 mm. The force of retention, the force necessary for removing the cap from the cone in the direction of removal, was 89,7 +/- 8,3 kp. Forces of rotation, 30 kp applied 100 times continuously, did not reduce the force of retention. Increasing the forces of rotation to 50 kp damage was cuased on the cement, resulting in removal of the cap from the cone after some applications. The possible clinical value of the investigation was discussed.", "contents": "[Retention status of crown/bridge constructions]. An apparatus consisting of an acid resistant steel cone and a cap of the same maternal, was used for retention and rotation experiments after cementation of the cap on the cone. The upper diameter of the cone was 6 mm, the lower diameter 7 mm and the height 6 mm. The force of retention, the force necessary for removing the cap from the cone in the direction of removal, was 89,7 +/- 8,3 kp. Forces of rotation, 30 kp applied 100 times continuously, did not reduce the force of retention. Increasing the forces of rotation to 50 kp damage was cuased on the cement, resulting in removal of the cap from the cone after some applications. The possible clinical value of the investigation was discussed."}
{"id": "PMID:1076826", "title": "Single-breath pulmonary diffusing capacity. Reference values and application in connective tissue diseases and in various lung diseases.", "content": "1.1. Diffusing capacity of the lungs was measured by using a Mark 4 Resparameter. The single-breath diffusing capacity (D) for carbon monoxide was found to be dependent on the lung volume (VA) during the breath-hold. The same applied to D/VA and to the time constant (tau) for carbon monoxide uptake. This confirms the theoretical considerations and the results of other investigators. This effect should be taken into account especially when making serial determinations of D on the same subject. 1.2. The repeatability of the method was found to be reasonably good; the coefficient of variation was about 4% in normal subjects, as well as in a series of patients with rheumatoid arthritis. 1.3. The effect of the correction for haemoglobin concentration was calculated and tabulated for a set of possible values of DM/Vc and haemoglobin. This correction did not reduce the scatter of D values in normal subjects, but it was adopted for clinical use for theoretical reasons. Caution in its use and interpretation of the correction is emphasized. 2.1. Healthy males and females, 20-69 years of age, were examined to establish reference values for the D measurement. Multiple linear regression equations were calculated stepwise, and equations based on age and height were then used in the clinical prediction equations. Similarly, prediction equations were calculated for clinical spirometry. 2.2. The prediction equations were compared with some published reference values. The equations provide a basis for the evaluation of the effect of various factors, such as age and smoking, in different populations. 3.1. Respiratory function, clinical symptoms and chest x-ray findings were examined in patients with connective tissue diseases. Three different investigations were thus formed: a) consecutive patients (free of lung disease other than that possibly due to a connective tissue disease) with definite rheumatoid arthritis (RA, 21 cases), systemic lupus erythematosus (SLE, 18 cases) or scleroderma (SCL, 6 cases) were subjected to detailed tests. b) 129 patients from the Rheumatism Foundation Hospital, Heinola, Finland, were subjected to measurement of diffusing capacity and vital capacity in addition to chest radiography and routine clinical assessment c) the histological findings on 12 patients subjected to a needle biopsy of the lung in order to exclude other conditions were compared with the results of diffusing capacity and x-ray examinations. 3.2. Restrictive impairment of the respiratory function was the general finding in all of these groups. The reduction in diffusing capacity was out of proportion to the reduction in lung volume, however, in most cases with abnormally low values. Low D values were encountered in about half of the RA and SLE and in all of the SCL patients in group a, and in 13% of the \"average\" RA patients studied in Heinola. The typical histological finding in patients with reduced D was a thickening of the alveolar wall...", "contents": "Single-breath pulmonary diffusing capacity. Reference values and application in connective tissue diseases and in various lung diseases. 1.1. Diffusing capacity of the lungs was measured by using a Mark 4 Resparameter. The single-breath diffusing capacity (D) for carbon monoxide was found to be dependent on the lung volume (VA) during the breath-hold. The same applied to D/VA and to the time constant (tau) for carbon monoxide uptake. This confirms the theoretical considerations and the results of other investigators. This effect should be taken into account especially when making serial determinations of D on the same subject. 1.2. The repeatability of the method was found to be reasonably good; the coefficient of variation was about 4% in normal subjects, as well as in a series of patients with rheumatoid arthritis. 1.3. The effect of the correction for haemoglobin concentration was calculated and tabulated for a set of possible values of DM/Vc and haemoglobin. This correction did not reduce the scatter of D values in normal subjects, but it was adopted for clinical use for theoretical reasons. Caution in its use and interpretation of the correction is emphasized. 2.1. Healthy males and females, 20-69 years of age, were examined to establish reference values for the D measurement. Multiple linear regression equations were calculated stepwise, and equations based on age and height were then used in the clinical prediction equations. Similarly, prediction equations were calculated for clinical spirometry. 2.2. The prediction equations were compared with some published reference values. The equations provide a basis for the evaluation of the effect of various factors, such as age and smoking, in different populations. 3.1. Respiratory function, clinical symptoms and chest x-ray findings were examined in patients with connective tissue diseases. Three different investigations were thus formed: a) consecutive patients (free of lung disease other than that possibly due to a connective tissue disease) with definite rheumatoid arthritis (RA, 21 cases), systemic lupus erythematosus (SLE, 18 cases) or scleroderma (SCL, 6 cases) were subjected to detailed tests. b) 129 patients from the Rheumatism Foundation Hospital, Heinola, Finland, were subjected to measurement of diffusing capacity and vital capacity in addition to chest radiography and routine clinical assessment c) the histological findings on 12 patients subjected to a needle biopsy of the lung in order to exclude other conditions were compared with the results of diffusing capacity and x-ray examinations. 3.2. Restrictive impairment of the respiratory function was the general finding in all of these groups. The reduction in diffusing capacity was out of proportion to the reduction in lung volume, however, in most cases with abnormally low values. Low D values were encountered in about half of the RA and SLE and in all of the SCL patients in group a, and in 13% of the \"average\" RA patients studied in Heinola. The typical histological finding in patients with reduced D was a thickening of the alveolar wall..."}
{"id": "PMID:1077318", "title": "Tumefactive extramedullary hematopoiesis of the stomach.", "content": "A 3 X 4 cm submucosal gastric mass of extramedullary hematopoeitic tissue occurred in a patient with chronic myelogenous leukemia. Such stomach masses have been misinterpreted as malignant tumor. Tumefactive extramedullary hematopoiesis of the stomach is an infrequent occurrence in patients with extramedullary hematopoiesis but should be considered in the differential diagnosis of patients known to have this condition.", "contents": "Tumefactive extramedullary hematopoiesis of the stomach. A 3 X 4 cm submucosal gastric mass of extramedullary hematopoeitic tissue occurred in a patient with chronic myelogenous leukemia. Such stomach masses have been misinterpreted as malignant tumor. Tumefactive extramedullary hematopoiesis of the stomach is an infrequent occurrence in patients with extramedullary hematopoiesis but should be considered in the differential diagnosis of patients known to have this condition."}
{"id": "PMID:1077761", "title": "beta-blocking drugs and renin secretion in the anaesthetized dog.", "content": "1. The effect of propranolol and sotalol on renin secretion was studied in the anaesthetized dog. 2. beta-adrenergic blockade did not modify basal renin secretion and did not affect the rise of renin secretion induced by haemorrhage or aortic constriction. 3. beta-adrenergic blockade diminished the rise in renin secretion induced by stimulation of the renal nerves.", "contents": "beta-blocking drugs and renin secretion in the anaesthetized dog. 1. The effect of propranolol and sotalol on renin secretion was studied in the anaesthetized dog. 2. beta-adrenergic blockade did not modify basal renin secretion and did not affect the rise of renin secretion induced by haemorrhage or aortic constriction. 3. beta-adrenergic blockade diminished the rise in renin secretion induced by stimulation of the renal nerves."}
{"id": "PMID:1077763", "title": "Plasma renin, urinary sodium excretion and vascular disease.", "content": "1. Urinary sodium excretion and plasma renin activity have been measured in a group of persons with untreated mild hypertension and in a control normotensive group. 2. Preliminary analyses of the data indicate that the daily sodium excretion was significantly higher in the hypertensive group but the plasma renin activity did not correlate significantly with the urinary excretion of sodium. 3. These findings suggest that sodium intake was significantly greater in a population with mild hypertension than in a comparable normotensive group.", "contents": "Plasma renin, urinary sodium excretion and vascular disease. 1. Urinary sodium excretion and plasma renin activity have been measured in a group of persons with untreated mild hypertension and in a control normotensive group. 2. Preliminary analyses of the data indicate that the daily sodium excretion was significantly higher in the hypertensive group but the plasma renin activity did not correlate significantly with the urinary excretion of sodium. 3. These findings suggest that sodium intake was significantly greater in a population with mild hypertension than in a comparable normotensive group."}
{"id": "PMID:1077764", "title": "Plasma renin activity and cardiovascular disease.", "content": "1. A group of patients with essential hypertension was divided into three categories on the basis of the plasma renin activity. 2. There was no correlation between the plasma renin activity categorized as high, normal or low and the duration of hypertension, the incidence of left ventricular enlargement, the blood urea nitrogen, serum creatinine, cholesterol or uric acid respectively. 3. Analysis of data showed that the incidence of cardiovascular events in the hypertensive population correlated with the plasma renin activity only in combination with known risk factors.", "contents": "Plasma renin activity and cardiovascular disease. 1. A group of patients with essential hypertension was divided into three categories on the basis of the plasma renin activity. 2. There was no correlation between the plasma renin activity categorized as high, normal or low and the duration of hypertension, the incidence of left ventricular enlargement, the blood urea nitrogen, serum creatinine, cholesterol or uric acid respectively. 3. Analysis of data showed that the incidence of cardiovascular events in the hypertensive population correlated with the plasma renin activity only in combination with known risk factors."}
{"id": "PMID:1077765", "title": "Inhibition of the pressor and aldosterone-releasing effects of angiotensin II.", "content": "1. Competitive or non-competitive inhibition of the myotropic and pressor response of angiotensin II is dependent on the nature of the substituent in position 8 of the antagonist peptide analogue. Substituents in other positions of the molecule, particularly position 1, contribute greatly to the potency of these antagonists. 2. As is evidenced after adrenalectomy or after blockade with phentolamine and phenoxybenzamine, the initial pressor activity observed with all the antagonistic peptides is partially due to the release of catecholamines and partially to a direct myotropic effect. 3. [Sar1, Thr8]angiotensin II has been found to possess the lowest agonist to antagonist ratio of all antagonists tested. 4. [Des-Asp1, Ile8]angiotensin II selectively and specifically inhibits the release of aldosterone from adrenal cortex. Thus, unlike angiotensin II, this heptapeptide has pronounced organ specificity, suggesting that the heptapeptide (angiotensin III) is the aldosterone-releasing hormone.", "contents": "Inhibition of the pressor and aldosterone-releasing effects of angiotensin II. 1. Competitive or non-competitive inhibition of the myotropic and pressor response of angiotensin II is dependent on the nature of the substituent in position 8 of the antagonist peptide analogue. Substituents in other positions of the molecule, particularly position 1, contribute greatly to the potency of these antagonists. 2. As is evidenced after adrenalectomy or after blockade with phentolamine and phenoxybenzamine, the initial pressor activity observed with all the antagonistic peptides is partially due to the release of catecholamines and partially to a direct myotropic effect. 3. [Sar1, Thr8]angiotensin II has been found to possess the lowest agonist to antagonist ratio of all antagonists tested. 4. [Des-Asp1, Ile8]angiotensin II selectively and specifically inhibits the release of aldosterone from adrenal cortex. Thus, unlike angiotensin II, this heptapeptide has pronounced organ specificity, suggesting that the heptapeptide (angiotensin III) is the aldosterone-releasing hormone."}
{"id": "PMID:1077766", "title": "Pathogenesis and prevention of stroke in spontaneously hypertensive rats.", "content": "1. A colony of stroke-prone spontaneously hypertensive rats has been developed by selective breeding. 2. These animals developed severe hypertension early in life, the magnitude of the hypertension being closely related to the incidence of stroke. 3. No evidence was obtained of any humoral factor responsible for strokes. 4. Local factors predisposing to stroke were a scanty arterial supply with characteristic recurrent branching of long and large arteries, together with increased vascular permeability, angio-necrosis, and formation of microaneurysms. 5. Strokes could be prevented by adequate antihypertensive therapy from an early age.", "contents": "Pathogenesis and prevention of stroke in spontaneously hypertensive rats. 1. A colony of stroke-prone spontaneously hypertensive rats has been developed by selective breeding. 2. These animals developed severe hypertension early in life, the magnitude of the hypertension being closely related to the incidence of stroke. 3. No evidence was obtained of any humoral factor responsible for strokes. 4. Local factors predisposing to stroke were a scanty arterial supply with characteristic recurrent branching of long and large arteries, together with increased vascular permeability, angio-necrosis, and formation of microaneurysms. 5. Strokes could be prevented by adequate antihypertensive therapy from an early age."}
{"id": "PMID:1077767", "title": "A pilot trial of treatment for mild hypertension; interim report of the Medical Research Council's trial in Britain.", "content": "Progress in the pilot phase of the British multicentre randomized controlled trial of treatment for patients with mild hypertension is described.", "contents": "A pilot trial of treatment for mild hypertension; interim report of the Medical Research Council's trial in Britain. Progress in the pilot phase of the British multicentre randomized controlled trial of treatment for patients with mild hypertension is described."}
{"id": "PMID:1077768", "title": "Yoga and biofeedback in the management of 'stress' in hypertensive patients.", "content": "1. Psychophysical relaxation exercises based on yogic principles and reinforced by biofeedback instruments were used for behaviour modification in sixteen hypertensive subjects. 2. Preliminary studies indicated that their pressor response to emotional and physical stimuli became less exaggerated and less protracted compared with controls.", "contents": "Yoga and biofeedback in the management of 'stress' in hypertensive patients. 1. Psychophysical relaxation exercises based on yogic principles and reinforced by biofeedback instruments were used for behaviour modification in sixteen hypertensive subjects. 2. Preliminary studies indicated that their pressor response to emotional and physical stimuli became less exaggerated and less protracted compared with controls."}
{"id": "PMID:1077769", "title": "Comparative pharmacology of new specific angiotensin antagonists.", "content": "1. The angiotensin II antagonism by newly synthesized 8-C-phenylglycine analogues of [5-isoleucine]angiotensin II in different preparations was investigated in vitro and in vivo. 2. All analogues competitively inhibited the myotropic effect of angiotensin II on the isolated colon ascendens of the guinea-pig and the stomach of the rat. 3. In normotensive dogs, cats, rabbits, guinea-pigs and rats the blood pressure response to infused angiotensin II was inhibited by the antagonists. The angiotensin II-induced fall in renal blood flow in the dog was blocked during infusion of the analogues. Acute renal hypertension in rats was significantly decreased. Of conscious rats variously with normal blood pressures, spontaneous hypertension and chronic renal hypertension, only in the last group could a marked uniform fall in blood pressure be demonstrated. The central pressor effect of angiotensin II was also inhibited in conscious rats. 4. 8-C-Phenylglycine analogues of [5-isoleucine]-angiotensin II exhibit a specific antagonistic activity to endogenous and exogenous angiotensin II.", "contents": "Comparative pharmacology of new specific angiotensin antagonists. 1. The angiotensin II antagonism by newly synthesized 8-C-phenylglycine analogues of [5-isoleucine]angiotensin II in different preparations was investigated in vitro and in vivo. 2. All analogues competitively inhibited the myotropic effect of angiotensin II on the isolated colon ascendens of the guinea-pig and the stomach of the rat. 3. In normotensive dogs, cats, rabbits, guinea-pigs and rats the blood pressure response to infused angiotensin II was inhibited by the antagonists. The angiotensin II-induced fall in renal blood flow in the dog was blocked during infusion of the analogues. Acute renal hypertension in rats was significantly decreased. Of conscious rats variously with normal blood pressures, spontaneous hypertension and chronic renal hypertension, only in the last group could a marked uniform fall in blood pressure be demonstrated. The central pressor effect of angiotensin II was also inhibited in conscious rats. 4. 8-C-Phenylglycine analogues of [5-isoleucine]-angiotensin II exhibit a specific antagonistic activity to endogenous and exogenous angiotensin II."}
{"id": "PMID:1077770", "title": "Adrenergic mechanisms in human hypertension and in spontaneously hypertensive rats.", "content": "1. The role of adrenergic neuronal mechanisms in the development of early hypertension in man and the spontaneously hypertensive rat has been explored. 2. In both, a hyperkinetic circulatory state is associated with reduced parasympathetic and increased adrenergic cardiac influences. 3. Spontaneously hypertensive and normotensive control rats were treated with propranolol from conception until 12 weeks. Although heart rate and output remained reduced, there was no difference in growth or elaboration of pressure with respect to their untreated controls. 4. After another series of spontaneously hypertensive and control rats received cardiac autonomic blockade (atropine and timolol), they sequentially received alpha-adrenergic blockade (phenoxybenzamine), ganglionic blockade (trimethaphan) and smooth-muscle vasodilatation (hydralazine). These studies revealed only a small pressure differential between the two groups before hydralazine and still less thereafter; unlike the control rats, pressure in spontaneously hypertensive rats fell markedly after ganglionic blockade as a result of reduced output, indicating greater adrenergic control mediated through venoconstriction. 5. These findings indicate: increased cardiovascular adrenergic control in young spontaneously hypertensive rats, the hyperkinetic circulation merely reflecting one aspect of increased total cardiovascular input. Structural alterations seem to participate minimally. 6. These experimental observations closely, resemble findings in early hypertensive man, and it is suggested that altered total cardiovascular adrenergic input is responsible for the elaboration, development, and maintenance of essential hypertension in man.", "contents": "Adrenergic mechanisms in human hypertension and in spontaneously hypertensive rats. 1. The role of adrenergic neuronal mechanisms in the development of early hypertension in man and the spontaneously hypertensive rat has been explored. 2. In both, a hyperkinetic circulatory state is associated with reduced parasympathetic and increased adrenergic cardiac influences. 3. Spontaneously hypertensive and normotensive control rats were treated with propranolol from conception until 12 weeks. Although heart rate and output remained reduced, there was no difference in growth or elaboration of pressure with respect to their untreated controls. 4. After another series of spontaneously hypertensive and control rats received cardiac autonomic blockade (atropine and timolol), they sequentially received alpha-adrenergic blockade (phenoxybenzamine), ganglionic blockade (trimethaphan) and smooth-muscle vasodilatation (hydralazine). These studies revealed only a small pressure differential between the two groups before hydralazine and still less thereafter; unlike the control rats, pressure in spontaneously hypertensive rats fell markedly after ganglionic blockade as a result of reduced output, indicating greater adrenergic control mediated through venoconstriction. 5. These findings indicate: increased cardiovascular adrenergic control in young spontaneously hypertensive rats, the hyperkinetic circulation merely reflecting one aspect of increased total cardiovascular input. Structural alterations seem to participate minimally. 6. These experimental observations closely, resemble findings in early hypertensive man, and it is suggested that altered total cardiovascular adrenergic input is responsible for the elaboration, development, and maintenance of essential hypertension in man."}
{"id": "PMID:1077771", "title": "The vasomotor centre and its afferent pathways.", "content": "1. Experiments were carried out on two groups of anaesthetized greyhounds. The response of the first group to infusions of angiotensin II into a vertebral artery for 5 min and to bilateral occlusion of the common carotid arteries for 2 min was studied before and after bilateral ablation of the areas postrema. The response of the second group to infusions of angiotensin and to carotid occlusion was studied before and after mid-collicular transection of the midbrain. 2. Bilateral ablation of the areas postrema abolished the response to angiotensin infused into a vertebral artery at the dose used but did not significantly alter the response to carotid occlusion. Transection of the midbrain did not abolish the response to either procedure. 3. It is concluded that the structures involved in the cardiovascular response to carotid occlusion are anatomically distinct from the area postrema and that neither response requires the integrity of connections above the pons. 4. An attempt has been made to construct a schematic diagram of the vasomotor centre.", "contents": "The vasomotor centre and its afferent pathways. 1. Experiments were carried out on two groups of anaesthetized greyhounds. The response of the first group to infusions of angiotensin II into a vertebral artery for 5 min and to bilateral occlusion of the common carotid arteries for 2 min was studied before and after bilateral ablation of the areas postrema. The response of the second group to infusions of angiotensin and to carotid occlusion was studied before and after mid-collicular transection of the midbrain. 2. Bilateral ablation of the areas postrema abolished the response to angiotensin infused into a vertebral artery at the dose used but did not significantly alter the response to carotid occlusion. Transection of the midbrain did not abolish the response to either procedure. 3. It is concluded that the structures involved in the cardiovascular response to carotid occlusion are anatomically distinct from the area postrema and that neither response requires the integrity of connections above the pons. 4. An attempt has been made to construct a schematic diagram of the vasomotor centre."}
{"id": "PMID:1077772", "title": "Control of high and low blood pressure in the dog by aortic and sinus nerves.", "content": "1. Changes in afferent activity in the aortic and sinus nerves with alterations of blood pressure were studied in anesthetized dogs. Mean aortic blood pressure was changed from 220 to 50 mmHg by using a pressurized reservoir connected to the abdominal aorta. 2. The stimulus--response curve (defined by measuring the mean impulse frequency at various pressures) from both nerves was S-shaped; the curve for the aortic nerve was shifted to the right of the sinus nerve curve. 3. In the dog, the aortic arch baroreceptors act predominantly as an anti-hypertensive mechanism; at lower pressures, the major control occurs through the sinus baroreflex.", "contents": "Control of high and low blood pressure in the dog by aortic and sinus nerves. 1. Changes in afferent activity in the aortic and sinus nerves with alterations of blood pressure were studied in anesthetized dogs. Mean aortic blood pressure was changed from 220 to 50 mmHg by using a pressurized reservoir connected to the abdominal aorta. 2. The stimulus--response curve (defined by measuring the mean impulse frequency at various pressures) from both nerves was S-shaped; the curve for the aortic nerve was shifted to the right of the sinus nerve curve. 3. In the dog, the aortic arch baroreceptors act predominantly as an anti-hypertensive mechanism; at lower pressures, the major control occurs through the sinus baroreflex."}
{"id": "PMID:1077773", "title": "Spinal sympathetic reflexes in the cat and the pathogenesis of arterial hypertension.", "content": "1. In vagotomized anaesthetized cats with both common carotid arteries occluded, stretch of the thoracic aorta induced reflex increases in arterial blood pressure, heart rate and left ventricular dP/dtmax. Similar responses were obtained in cats with spinal transection at the level of the first cervical nerve roots. 2. The hypothesis is advanced that sympathetic excitatory reflexes may contribute to the maintenance of hypertension through positive feedback.", "contents": "Spinal sympathetic reflexes in the cat and the pathogenesis of arterial hypertension. 1. In vagotomized anaesthetized cats with both common carotid arteries occluded, stretch of the thoracic aorta induced reflex increases in arterial blood pressure, heart rate and left ventricular dP/dtmax. Similar responses were obtained in cats with spinal transection at the level of the first cervical nerve roots. 2. The hypothesis is advanced that sympathetic excitatory reflexes may contribute to the maintenance of hypertension through positive feedback."}
{"id": "PMID:1077774", "title": "Carotid baroceptor re-setting in the hypertensive dog.", "content": "1. The behaviour of forty-four single carotid baroreceptor fibres from nine dogs with normal blood pressure was contrasted with that of forty-seven single fibres from nine dogs with renal (nephrectomy--clip) hypertension. 2. It was found that in response to a square-wave pressure step, the mean steady-state threshold pressure was 99.23 mmHg for the normotensive dogs and 129.13 mmHg for the hypertensive dogs. 3. The degree of re-setting appeared to correlate with the duration of the hypertension but was not seen until an animal had been hypertensive for more than 4 days. 4. In one animal, hypertensive for 7 weeks but whose blood pressure returned towards normal after removal of the clip, the receptor characteristics was normal. 5. No change in the gain of the receptor sensitivity was seen. 6. Light-microscopy and electron miscroscopy of the opposite (non-perfused) carotid sinus revealed no differences in the fibre morphology or density between the hypertensive and normotensive animals (a random sampling, four normal and six hypertensive).", "contents": "Carotid baroceptor re-setting in the hypertensive dog. 1. The behaviour of forty-four single carotid baroreceptor fibres from nine dogs with normal blood pressure was contrasted with that of forty-seven single fibres from nine dogs with renal (nephrectomy--clip) hypertension. 2. It was found that in response to a square-wave pressure step, the mean steady-state threshold pressure was 99.23 mmHg for the normotensive dogs and 129.13 mmHg for the hypertensive dogs. 3. The degree of re-setting appeared to correlate with the duration of the hypertension but was not seen until an animal had been hypertensive for more than 4 days. 4. In one animal, hypertensive for 7 weeks but whose blood pressure returned towards normal after removal of the clip, the receptor characteristics was normal. 5. No change in the gain of the receptor sensitivity was seen. 6. Light-microscopy and electron miscroscopy of the opposite (non-perfused) carotid sinus revealed no differences in the fibre morphology or density between the hypertensive and normotensive animals (a random sampling, four normal and six hypertensive)."}
{"id": "PMID:1077775", "title": "The intrinsic brain iso-renin--angiotensin system in the rat: its possible role in central mechanisms of blood pressure regulation.", "content": "1. Angiotensin is produced by the intrinsic isorenin--angiotensin system. 2. Angiotensin is secreted into the cerebrospinal fluid of nephrectomized rats. 3. Angiotensin in cerebrospinal fluid elevates systemic blood pressure. 4. Rats with hereditary diabetes insipidus are virtually non-responsive to intraventricular angiotensin. 5. Angiotensin II is elevated in the cerebrospinal fluid of spontaneously hypertensive rats. 6. An intraventricular perfusion of the angiotensin II receptor-blocking agent P 113 decreases blood pressure in spontaneously hypertensive rats.", "contents": "The intrinsic brain iso-renin--angiotensin system in the rat: its possible role in central mechanisms of blood pressure regulation. 1. Angiotensin is produced by the intrinsic isorenin--angiotensin system. 2. Angiotensin is secreted into the cerebrospinal fluid of nephrectomized rats. 3. Angiotensin in cerebrospinal fluid elevates systemic blood pressure. 4. Rats with hereditary diabetes insipidus are virtually non-responsive to intraventricular angiotensin. 5. Angiotensin II is elevated in the cerebrospinal fluid of spontaneously hypertensive rats. 6. An intraventricular perfusion of the angiotensin II receptor-blocking agent P 113 decreases blood pressure in spontaneously hypertensive rats."}
{"id": "PMID:1077776", "title": "The response of the renin--angiotensin systems in rats to the injection of angiotensin II antibodies.", "content": "1. Antibodies against angiotensin II were purified by affinity chromatography. 2. When injected intravenously into rats, the antibody distributed in the extracellular space with a half-time of 11 h and a distribution volume of about 10 ml/100 g body weight. The antibody was eliminated with a half-time of 7 days. 3. Plasma angiotensin II concentrations increased about 100-fold the control values 7 min after antibody injection and declined in parallel with the antibody concentration. It was calculated that only about 1--4% of the binding capacity of the antibody was occupied by angiotensin throughout the experiment. 4. Since the plasma renin concentration was normal, except during the short initial phase of stimulation, it is concluded that upon antibody injection the renin-angiotensin system rapidly reached an equilibrium, with concentrations of free angiotensin close to or identical with normal concentrations.", "contents": "The response of the renin--angiotensin systems in rats to the injection of angiotensin II antibodies. 1. Antibodies against angiotensin II were purified by affinity chromatography. 2. When injected intravenously into rats, the antibody distributed in the extracellular space with a half-time of 11 h and a distribution volume of about 10 ml/100 g body weight. The antibody was eliminated with a half-time of 7 days. 3. Plasma angiotensin II concentrations increased about 100-fold the control values 7 min after antibody injection and declined in parallel with the antibody concentration. It was calculated that only about 1--4% of the binding capacity of the antibody was occupied by angiotensin throughout the experiment. 4. Since the plasma renin concentration was normal, except during the short initial phase of stimulation, it is concluded that upon antibody injection the renin-angiotensin system rapidly reached an equilibrium, with concentrations of free angiotensin close to or identical with normal concentrations."}
{"id": "PMID:1077778", "title": "Central serotonergic neurons and experimental neurogenic and renal hypertension in the rabbit.", "content": "1. Rabbits received intracisternal injections of 5,6-dihydroxytryptamine (5,6-DHT) in order to ablate central serotonergic nerves and deplete central serotonin stores. Depletion was most marked in the spinal cord where serotonin concentration was reduced to less than 50% of that in rabbits given control injections. 2. In normal rabbits, intracisternal 5,6-DHT caused a transient reduction in arterial pressure, which was maximal 1 week after injection. 3. Intracisternal 5,6-DHT completely prevented the neurogenic hypertension produced by sinoaortic denervation in control animals and reversed it when given after sustained neurogenic hypertension had developed in denervated animals. These studies suggest that central serotonergic neurons participate in the baroreceptor reflex arc. 4. Intracisternal 5,6-DHT did not modify the renal hypertension that follows bilateral renal wrapping.", "contents": "Central serotonergic neurons and experimental neurogenic and renal hypertension in the rabbit. 1. Rabbits received intracisternal injections of 5,6-dihydroxytryptamine (5,6-DHT) in order to ablate central serotonergic nerves and deplete central serotonin stores. Depletion was most marked in the spinal cord where serotonin concentration was reduced to less than 50% of that in rabbits given control injections. 2. In normal rabbits, intracisternal 5,6-DHT caused a transient reduction in arterial pressure, which was maximal 1 week after injection. 3. Intracisternal 5,6-DHT completely prevented the neurogenic hypertension produced by sinoaortic denervation in control animals and reversed it when given after sustained neurogenic hypertension had developed in denervated animals. These studies suggest that central serotonergic neurons participate in the baroreceptor reflex arc. 4. Intracisternal 5,6-DHT did not modify the renal hypertension that follows bilateral renal wrapping."}
{"id": "PMID:1077780", "title": "Dopa, catecholamines and their metabolites in essential hypertension.", "content": "1. Urinary excretion of dopa, catecholamines and their metabolites (vanillylmandelic acid, methoxycatecholamines, 3-methoxy-4-hydroxyphenylglycol and homovanillic acid) were studied in eighty patients with essential hypertension and in twenty-five healthy control subjects. 2. Increased urinary excretion of catecholamines, dopa and catecholamine metabolites was found in a proportion of cases. 3. The relationship between urinary excretion of catecholamine metabolites and the excretion of dopa and noradrenaline was studied. 4. In view of the suggested significance of 3-methoxy-4-hydroxyphenylglycol as an index of brain catecholamine metabolism, particular attention was paid to urinary excretion of this metabolite in the subjects under study.", "contents": "Dopa, catecholamines and their metabolites in essential hypertension. 1. Urinary excretion of dopa, catecholamines and their metabolites (vanillylmandelic acid, methoxycatecholamines, 3-methoxy-4-hydroxyphenylglycol and homovanillic acid) were studied in eighty patients with essential hypertension and in twenty-five healthy control subjects. 2. Increased urinary excretion of catecholamines, dopa and catecholamine metabolites was found in a proportion of cases. 3. The relationship between urinary excretion of catecholamine metabolites and the excretion of dopa and noradrenaline was studied. 4. In view of the suggested significance of 3-methoxy-4-hydroxyphenylglycol as an index of brain catecholamine metabolism, particular attention was paid to urinary excretion of this metabolite in the subjects under study."}
{"id": "PMID:1077781", "title": "Effect of a competitive angiotensin antagonist on the renal haemodynamic changes induced by inhibition of prostaglandin synthesis in rats.", "content": "1. Inhibition of prostaglandin synthesis by indomethacin induced an increase in blood pressure which did not occur when rats were bilaterally nephrectomized. 2. The blood pressure effect was related to the state of sodium balance and thus to the activity of the renin--angiotensin system. 3. Indomethacin induced a decrease in renal blood flow. 4. Angiotensin receptor blockade with Sar1-Ala8-angiotensin II blunted the blood pressure effect and prevented the renal haemodynamic changes induced by indomethacin.", "contents": "Effect of a competitive angiotensin antagonist on the renal haemodynamic changes induced by inhibition of prostaglandin synthesis in rats. 1. Inhibition of prostaglandin synthesis by indomethacin induced an increase in blood pressure which did not occur when rats were bilaterally nephrectomized. 2. The blood pressure effect was related to the state of sodium balance and thus to the activity of the renin--angiotensin system. 3. Indomethacin induced a decrease in renal blood flow. 4. Angiotensin receptor blockade with Sar1-Ala8-angiotensin II blunted the blood pressure effect and prevented the renal haemodynamic changes induced by indomethacin."}
{"id": "PMID:1077782", "title": "Renal prostaglandin synthesis in experimental renal-clip hypertension in the rat.", "content": "1. The hypothesis that suppression of prostaglandin E synthesis by indomethacin exacerbates renal-clip hypertension in the rat was investigated in four groups of rats. 2. Indomethacin was shown to exacerbate hypertension in renal-clip animals. 3. Synthesis of prostaglandin E, determined by gas--liquid chromatography, was suppressed in medullary tissue from the hypertensive animals irrespective of indomethacin treatment. 4. The findings support the concept that prostaglandins participate in the blood pressure regulatory function of the kidney but pose a number of unsolved questions.", "contents": "Renal prostaglandin synthesis in experimental renal-clip hypertension in the rat. 1. The hypothesis that suppression of prostaglandin E synthesis by indomethacin exacerbates renal-clip hypertension in the rat was investigated in four groups of rats. 2. Indomethacin was shown to exacerbate hypertension in renal-clip animals. 3. Synthesis of prostaglandin E, determined by gas--liquid chromatography, was suppressed in medullary tissue from the hypertensive animals irrespective of indomethacin treatment. 4. The findings support the concept that prostaglandins participate in the blood pressure regulatory function of the kidney but pose a number of unsolved questions."}
{"id": "PMID:1077783", "title": "Effect of prostaglandins (E2 and A2) on the enzymatic reaction of human renin in isolated homologous system and with added normal and hypertensive plasma.", "content": "1. Prostaglandin E2 significantly inhibits the renin reaction in whole plasma as well as in the isolated system of semi-purified human renin and human renin substrate. The inhibitory effect of prostaglandin A2 was less marked in whole plasma and absent in the isolated system. 2. The inhibitory effect of prostaglandin E2 was more marked in normal than hypertensive plasma and was maximal at the lowest concentration used. In hypertensive plasma the maximal inhibitory effect was achieved at tenfold higher concentrations. 3. In normal plasma prostaglandin E2 does not affect the rate of product formation (k5 = k6), but inhibits the overall renin reaction by decreasing the total amount of available enzyme-substrate and enzyme--substrate modifier complex (K2 less than K3). 4. In hypertensive plasma prostaglandin E2 acts as a potential accelerator of the rate of product formation (k6 greater than k5). In the range of substrate concentration employed, the apparent inhibitory effect is explained by an even greater lack of available complex (K less than K3). This behaviour in hypertensive plasma is consistent with the presence of an additional modifier (? activator).", "contents": "Effect of prostaglandins (E2 and A2) on the enzymatic reaction of human renin in isolated homologous system and with added normal and hypertensive plasma. 1. Prostaglandin E2 significantly inhibits the renin reaction in whole plasma as well as in the isolated system of semi-purified human renin and human renin substrate. The inhibitory effect of prostaglandin A2 was less marked in whole plasma and absent in the isolated system. 2. The inhibitory effect of prostaglandin E2 was more marked in normal than hypertensive plasma and was maximal at the lowest concentration used. In hypertensive plasma the maximal inhibitory effect was achieved at tenfold higher concentrations. 3. In normal plasma prostaglandin E2 does not affect the rate of product formation (k5 = k6), but inhibits the overall renin reaction by decreasing the total amount of available enzyme-substrate and enzyme--substrate modifier complex (K2 less than K3). 4. In hypertensive plasma prostaglandin E2 acts as a potential accelerator of the rate of product formation (k6 greater than k5). In the range of substrate concentration employed, the apparent inhibitory effect is explained by an even greater lack of available complex (K less than K3). This behaviour in hypertensive plasma is consistent with the presence of an additional modifier (? activator)."}
{"id": "PMID:1077784", "title": "Blockade of renin release by lanthanum.", "content": "1. The effects of lanthanum on renin release and renal vasoconstriction were studied in the isolated perfused rat kidney. 2. Lanthanum reduced noradrenaline-induced renal vasoconstriction. 3. Lanthanum prevented isoprenaline-induced and glucagon-induced stimulation of renin secretion.", "contents": "Blockade of renin release by lanthanum. 1. The effects of lanthanum on renin release and renal vasoconstriction were studied in the isolated perfused rat kidney. 2. Lanthanum reduced noradrenaline-induced renal vasoconstriction. 3. Lanthanum prevented isoprenaline-induced and glucagon-induced stimulation of renin secretion."}
{"id": "PMID:1077786", "title": "Angiotensin blockade in studies of the feedback control of renin release in rats and rabbits.", "content": "1. In rabbits actively immunized against angiotensin II (AII), the appearance of anti-AII antibodies was associated with a rise in plasma renin activity (PRA), which did not occur in mock-immunized controls. 2. In conscious rabbits, infusion of the angiotensin inhibitor, Sar1-Ala8-angiotensin II (P-113), at rates of 0.055, 0.22 or 1.1 nmol min-1 kg-1 into the renal artery, caused dose-related increases in arterial PRA and renal arteriovenous PRA difference. Renal blood flow fell with the high dose, but not with the low or medium doses. A fall in arterial pressure, asynchronous with peak renin secretion, accompanied the medium- and high-dose infusions. 3. Intravenous infusion of inhibitor P-113, 5.5 nmol min-1 kg-1, into anaesthetized rats produced highly significant increases in PRA and plasma renin concentration without reduction in arterial pressure. There were no changes in PRA or plasma renin concentration in saline-infused control rats. 4. These findings suggest that AII blockade interrupts a negative feedback loop controlling renin secretion.", "contents": "Angiotensin blockade in studies of the feedback control of renin release in rats and rabbits. 1. In rabbits actively immunized against angiotensin II (AII), the appearance of anti-AII antibodies was associated with a rise in plasma renin activity (PRA), which did not occur in mock-immunized controls. 2. In conscious rabbits, infusion of the angiotensin inhibitor, Sar1-Ala8-angiotensin II (P-113), at rates of 0.055, 0.22 or 1.1 nmol min-1 kg-1 into the renal artery, caused dose-related increases in arterial PRA and renal arteriovenous PRA difference. Renal blood flow fell with the high dose, but not with the low or medium doses. A fall in arterial pressure, asynchronous with peak renin secretion, accompanied the medium- and high-dose infusions. 3. Intravenous infusion of inhibitor P-113, 5.5 nmol min-1 kg-1, into anaesthetized rats produced highly significant increases in PRA and plasma renin concentration without reduction in arterial pressure. There were no changes in PRA or plasma renin concentration in saline-infused control rats. 4. These findings suggest that AII blockade interrupts a negative feedback loop controlling renin secretion."}
{"id": "PMID:1077788", "title": "Effect of angiotensin II and of an angiotensin II analogue (Sar1-Ile8-angiotensin II) on blood pressure, plasma aldosterone and plasma renin activity in the dog.", "content": "1. The effect of infusions of equimolar doses of angiotensin II (AII) and of the angiotensin analogue Sar1-Ile8-angiotensin II on arterial blood pressure, plasma aldosterone and plasma renin activity were compared in normal anaesthetized dexamethasone suppressed dogs. 2. Angiotensin II induced a significant increase of blood pressure and of plasma aldosterone whereas plasma renin activity decreased. The blood pressure was only slightly affected by large doses of the analogue. Plasma aldosterone, however, increased and plasma renin activity decreased. These changes were significant but less pronounced than after the infusions of angiotensin II. Plasma aldosterone remained high and renin activity low for 40 min after the infusions of the analogue. 3. The results suggest a strong agonistic potency of Sar1-Ile8-angiotensin II at the adrenal and renal angiotensin receptors, and that it is almost ineffective at the vascular receptors. The inhibition of renin secretion by angiotensin seems not be related to its vasoconstrictive activity.", "contents": "Effect of angiotensin II and of an angiotensin II analogue (Sar1-Ile8-angiotensin II) on blood pressure, plasma aldosterone and plasma renin activity in the dog. 1. The effect of infusions of equimolar doses of angiotensin II (AII) and of the angiotensin analogue Sar1-Ile8-angiotensin II on arterial blood pressure, plasma aldosterone and plasma renin activity were compared in normal anaesthetized dexamethasone suppressed dogs. 2. Angiotensin II induced a significant increase of blood pressure and of plasma aldosterone whereas plasma renin activity decreased. The blood pressure was only slightly affected by large doses of the analogue. Plasma aldosterone, however, increased and plasma renin activity decreased. These changes were significant but less pronounced than after the infusions of angiotensin II. Plasma aldosterone remained high and renin activity low for 40 min after the infusions of the analogue. 3. The results suggest a strong agonistic potency of Sar1-Ile8-angiotensin II at the adrenal and renal angiotensin receptors, and that it is almost ineffective at the vascular receptors. The inhibition of renin secretion by angiotensin seems not be related to its vasoconstrictive activity."}
{"id": "PMID:1077790", "title": "The role of the renin--angiotensin--aldosterone system in cardiovascular homeostasis in normal man.", "content": "1. To examine the role of angiotensin II in the maintenance of blood pressure and control of aldosterone secretion, eight normal human subjects were studied on a tilt table in sodium-replete and sodium-depleted states, before and after the administration of an angiotensin converting-enzyme inhibitor (CEI). 2. Administration of CEI was followed by a marked fall in blood pressure on tilting in sodium-depleted, but not in sodium-replete, subjects. CEI administration also resulted in a rise in plasma renin activity in the supine position, in the absence of haemodynamic change. The rise in plasma aldosterone observed both in response to tilting and sodium depletion did not occur after CEI, even though plasma renin activities were higher. 3. These results indicate that: (a) angiotensin II is essential for blood pressure control in the sodium-depleted individual; (b) angiotensin II exerts direct feedback control on renin secretion; (c) angiotensin II is the primary stimulus to aldosterone secretion in response to both sodium depletion and posture.", "contents": "The role of the renin--angiotensin--aldosterone system in cardiovascular homeostasis in normal man. 1. To examine the role of angiotensin II in the maintenance of blood pressure and control of aldosterone secretion, eight normal human subjects were studied on a tilt table in sodium-replete and sodium-depleted states, before and after the administration of an angiotensin converting-enzyme inhibitor (CEI). 2. Administration of CEI was followed by a marked fall in blood pressure on tilting in sodium-depleted, but not in sodium-replete, subjects. CEI administration also resulted in a rise in plasma renin activity in the supine position, in the absence of haemodynamic change. The rise in plasma aldosterone observed both in response to tilting and sodium depletion did not occur after CEI, even though plasma renin activities were higher. 3. These results indicate that: (a) angiotensin II is essential for blood pressure control in the sodium-depleted individual; (b) angiotensin II exerts direct feedback control on renin secretion; (c) angiotensin II is the primary stimulus to aldosterone secretion in response to both sodium depletion and posture."}
{"id": "PMID:1077791", "title": "Treatment of patients with severe hypertension by inhibition of angiotensin-converting enzyme.", "content": "1. The results of the administration of the nona-peptide inhibitor (SQ 20,881) of the enzymatic conversion of angiotensin I into angiotensin II in twelve severe hypertensive patients are presented. 2. Administration of the compound was associated with a fall in blood pressure in ten of twelve patients. 3. Four patients had a normal plasma renin activity (PRA) with a range of 1.6-3.7 ng h-1 ml-1 and eight patients had a high PRA with a range of 5.0-74 ng h-1 ml-1. Two of the patients with normal PRA had no fall in blood pressure despite receiving 2 mg/kg of the compound. Two patients with normal PRA, however, did respond, thus indicating that a high PRA is not necessary for a response to the inhibitor compound. 4. It was found that haemodialysis or diuresis with frusemide enhanced the blood pressure response to the compound. 5. The presence of a measured low total blood volume was found to be associated with an exaggerated fall in blood pressure to a small dose of compound (0.125 mg/kg) in one patient.", "contents": "Treatment of patients with severe hypertension by inhibition of angiotensin-converting enzyme. 1. The results of the administration of the nona-peptide inhibitor (SQ 20,881) of the enzymatic conversion of angiotensin I into angiotensin II in twelve severe hypertensive patients are presented. 2. Administration of the compound was associated with a fall in blood pressure in ten of twelve patients. 3. Four patients had a normal plasma renin activity (PRA) with a range of 1.6-3.7 ng h-1 ml-1 and eight patients had a high PRA with a range of 5.0-74 ng h-1 ml-1. Two of the patients with normal PRA had no fall in blood pressure despite receiving 2 mg/kg of the compound. Two patients with normal PRA, however, did respond, thus indicating that a high PRA is not necessary for a response to the inhibitor compound. 4. It was found that haemodialysis or diuresis with frusemide enhanced the blood pressure response to the compound. 5. The presence of a measured low total blood volume was found to be associated with an exaggerated fall in blood pressure to a small dose of compound (0.125 mg/kg) in one patient."}
{"id": "PMID:1077792", "title": "The use of angiotensin-converting enzyme inhibitor in the diagnosis and treatment of hypertension.", "content": "1. The anti-hypertensive effect of converting enzyme inhibition was evaluated in twenty-three hypertensive patients (seven renovascular, four essential, four malignant, one scleroderma, three chronic renal failure, four primary or idiopathic aldosteronism). 2. In sixteen patients a single injection (1--4 mg/kg) of the inhibitor produced an immediate anti-hypertensive effect, which lasted up to 16 h. In six patients the anti-hypertensive effect of the inhibitor was significantly improved after sodium depletion. 3. Plasma renin activities increased and plasma aldosterone concentrations decreased consistently except in idiopathic aldosteronism. 4. Converting enzyme inhibition provides a direct way of defining the degree of renin-dependency of the hypertension. Accordingly, it can be used diagnostically and for planning appropriate therapy. Therapeutically, it could be advantageous in hypertensive emergencies because of its safety, specificity and capacity to reduce aldosterone secretion.", "contents": "The use of angiotensin-converting enzyme inhibitor in the diagnosis and treatment of hypertension. 1. The anti-hypertensive effect of converting enzyme inhibition was evaluated in twenty-three hypertensive patients (seven renovascular, four essential, four malignant, one scleroderma, three chronic renal failure, four primary or idiopathic aldosteronism). 2. In sixteen patients a single injection (1--4 mg/kg) of the inhibitor produced an immediate anti-hypertensive effect, which lasted up to 16 h. In six patients the anti-hypertensive effect of the inhibitor was significantly improved after sodium depletion. 3. Plasma renin activities increased and plasma aldosterone concentrations decreased consistently except in idiopathic aldosteronism. 4. Converting enzyme inhibition provides a direct way of defining the degree of renin-dependency of the hypertension. Accordingly, it can be used diagnostically and for planning appropriate therapy. Therapeutically, it could be advantageous in hypertensive emergencies because of its safety, specificity and capacity to reduce aldosterone secretion."}
{"id": "PMID:1078257", "title": "Routine abscess tonsillectomy: late results.", "content": "The late results of abscess tonsillectomy as a routine treatment of peritonsillar abscess were investigated. The material comprises 113 patients. Follow-up was performed two to five years after the operation (bilateral dissection tonsillectomy under general anesthesia). Symptoms of pharyngitis, recurrent or chronic, were present in 17 percent of the patients, in most cases without major objective changes in the throat. The incidence of these symptoms was highest--70 percent--in patients past middle age without any history of trouble from the throat before the peritonsillar abscess. Tonsil remnants were seen in 28 percent, but only 6 percent of the patients had new episodes of febrile throat infections. The results are discussed. A reserved attitude to abscess tonsillectomy (and to interval tonsillectomy) is recommended for peritonsillar abscess in elderly patients without previous trouble from the throat.", "contents": "Routine abscess tonsillectomy: late results. The late results of abscess tonsillectomy as a routine treatment of peritonsillar abscess were investigated. The material comprises 113 patients. Follow-up was performed two to five years after the operation (bilateral dissection tonsillectomy under general anesthesia). Symptoms of pharyngitis, recurrent or chronic, were present in 17 percent of the patients, in most cases without major objective changes in the throat. The incidence of these symptoms was highest--70 percent--in patients past middle age without any history of trouble from the throat before the peritonsillar abscess. Tonsil remnants were seen in 28 percent, but only 6 percent of the patients had new episodes of febrile throat infections. The results are discussed. A reserved attitude to abscess tonsillectomy (and to interval tonsillectomy) is recommended for peritonsillar abscess in elderly patients without previous trouble from the throat."}
{"id": "PMID:1078264", "title": "[Cerebrovascular disorders in the fascicular block picture on the electrocardiogram].", "content": "3894 ECG of neuropsychiatric patients were investigated for mono- and bifascicular blocks and the charts taken out for closer examination. Within this group of patients, the percentage of cerebral circulatory disturbances proved to be significantly increased. Among 97 patients there were 15 suffering from syncopal attacks. These attacks seem to be caused by intermittent occurrence of a trifascicular block. In a randomized control group of 50 patients not a single syncopal attack was found. The risk for a patient to develop syncopal attacks is correlated to the advancing degree of blockage in the ECG. When an intermittent trifascicular block is detected in a patient suffering from syncopal attacks, an electric pacemaker should be implanted.", "contents": "[Cerebrovascular disorders in the fascicular block picture on the electrocardiogram]. 3894 ECG of neuropsychiatric patients were investigated for mono- and bifascicular blocks and the charts taken out for closer examination. Within this group of patients, the percentage of cerebral circulatory disturbances proved to be significantly increased. Among 97 patients there were 15 suffering from syncopal attacks. These attacks seem to be caused by intermittent occurrence of a trifascicular block. In a randomized control group of 50 patients not a single syncopal attack was found. The risk for a patient to develop syncopal attacks is correlated to the advancing degree of blockage in the ECG. When an intermittent trifascicular block is detected in a patient suffering from syncopal attacks, an electric pacemaker should be implanted."}
{"id": "PMID:1078265", "title": "[Ventricular arrhythmias in reduced glycoside tolerance and digitalis poisoning together with myocardial infarct].", "content": "Electrophysiological fundamental principles of the heart muscle cell and the conduction system are discussed in detail. Basic differences are described. Pathophysiology of ventricular arrhythmias is emphasized, especially the ectopic conduction development and conduction defect of the reentry mechanism. Decreased digitalis tolerance of different causes and overdosage of digitalis may lead to ventricular premature heart beats and tachycardias. Myocardial infarction is discussed in detail because lethality within the first hours of infarction is mostly caused by ventricular fibrillation.", "contents": "[Ventricular arrhythmias in reduced glycoside tolerance and digitalis poisoning together with myocardial infarct]. Electrophysiological fundamental principles of the heart muscle cell and the conduction system are discussed in detail. Basic differences are described. Pathophysiology of ventricular arrhythmias is emphasized, especially the ectopic conduction development and conduction defect of the reentry mechanism. Decreased digitalis tolerance of different causes and overdosage of digitalis may lead to ventricular premature heart beats and tachycardias. Myocardial infarction is discussed in detail because lethality within the first hours of infarction is mostly caused by ventricular fibrillation."}
{"id": "PMID:1078266", "title": "[ECG changes and peripheral blood pressure during endoscopic study of the stomach].", "content": "It is reported on alterations of ECG and blood pressure appearing in 51 patients during endoscopic examination of the stomach. Above all, these alterations consisted of repolarisation disturbances with a partial preterminal negative T and isolated supraventricular extrasystoles. Atrioventricular blocks or spreading of the QRS-complex were not observed. In all patients a distinct increase in frequency was present while the endoscope was introduced. Since regression of all these alterations would occur after examination, a vegetative-functional stimulus might be the inducing factor. In endoscopic examinations the risk of any incidents appearing in connection with the system of coronary circulation is minimal. In patients with considerable previous damage of the heart caution is advised.", "contents": "[ECG changes and peripheral blood pressure during endoscopic study of the stomach]. It is reported on alterations of ECG and blood pressure appearing in 51 patients during endoscopic examination of the stomach. Above all, these alterations consisted of repolarisation disturbances with a partial preterminal negative T and isolated supraventricular extrasystoles. Atrioventricular blocks or spreading of the QRS-complex were not observed. In all patients a distinct increase in frequency was present while the endoscope was introduced. Since regression of all these alterations would occur after examination, a vegetative-functional stimulus might be the inducing factor. In endoscopic examinations the risk of any incidents appearing in connection with the system of coronary circulation is minimal. In patients with considerable previous damage of the heart caution is advised."}
{"id": "PMID:1078268", "title": "[Endoscopic biopsy using the electrocautery snare (macro particle biopsy)].", "content": "The biopsy snare has greatly improved the diagnostic and therapeutic possibilities in gastro-intestinal endoscopy. Experience with endoscopic polypectomy in the digestive tract has stimulated the use of the biopsy snare for the excision of larger particles. Thus, besides that larger particles can be obtained, also deeper excisions are possible. Usually muscularis mucosae and parts of the submucosal layers are included. Mucosal hyperplasia and submucosal processes can be recognized and analysed preoperatively. Even the resection of inoperable stenosing neoplasms is now possible. The results of 93 gastro-intestinal big particle biopsies are reported. The risk of big particle biopsy proved to be minimal; once bleeding occurred and was managed conservatively.", "contents": "[Endoscopic biopsy using the electrocautery snare (macro particle biopsy)]. The biopsy snare has greatly improved the diagnostic and therapeutic possibilities in gastro-intestinal endoscopy. Experience with endoscopic polypectomy in the digestive tract has stimulated the use of the biopsy snare for the excision of larger particles. Thus, besides that larger particles can be obtained, also deeper excisions are possible. Usually muscularis mucosae and parts of the submucosal layers are included. Mucosal hyperplasia and submucosal processes can be recognized and analysed preoperatively. Even the resection of inoperable stenosing neoplasms is now possible. The results of 93 gastro-intestinal big particle biopsies are reported. The risk of big particle biopsy proved to be minimal; once bleeding occurred and was managed conservatively."}
{"id": "PMID:1078269", "title": "[Pathophysiologic significance of small intestine flora in the stagnant loop syndrome].", "content": "A report is given about a 53 years old man with a \"stagnant loop syndrome\" associated with a diverticulosis of the whole small intestine and colon. Steatorrhoea was the main clinical symptom. The classification, distribution and frequency of intestinal diverticulosis is based on radiological results. The pathological values of the absorption tests prompted for a discussion of new examinations in the \"stagnant loop syndrome\". The property of the intestinal bacteria to influence the absorption of fat, xylose and vitamin B12 is stressed. Finally the possible methods of treating the diverticulosis with \"stagnant loop syndrome\" are discussed.", "contents": "[Pathophysiologic significance of small intestine flora in the stagnant loop syndrome]. A report is given about a 53 years old man with a \"stagnant loop syndrome\" associated with a diverticulosis of the whole small intestine and colon. Steatorrhoea was the main clinical symptom. The classification, distribution and frequency of intestinal diverticulosis is based on radiological results. The pathological values of the absorption tests prompted for a discussion of new examinations in the \"stagnant loop syndrome\". The property of the intestinal bacteria to influence the absorption of fat, xylose and vitamin B12 is stressed. Finally the possible methods of treating the diverticulosis with \"stagnant loop syndrome\" are discussed."}
{"id": "PMID:1078270", "title": "[Joint occurrence of spinal anomalies and colonic diverticula].", "content": "We investigated the radiographs of 125 patients with respect to variations of intestines (diverticula, polyps, carcinomas) and simultaneous anomalies in vertebral column (fissure of vertebral arches, asymmetric growth of vertebral arches, deterioration of ribs, fusing of vertebrae). There is a significant correlation between diverticula of colon and anomalies in vertebral column, whereas we found no correlation between other variations of intestines (polyps, carcinomas) and anomalies in vertebral columns. It is according to the theory of syndrome anomalies if there is an embryogenesis relation between anomalies of intestines and vertebral column.", "contents": "[Joint occurrence of spinal anomalies and colonic diverticula]. We investigated the radiographs of 125 patients with respect to variations of intestines (diverticula, polyps, carcinomas) and simultaneous anomalies in vertebral column (fissure of vertebral arches, asymmetric growth of vertebral arches, deterioration of ribs, fusing of vertebrae). There is a significant correlation between diverticula of colon and anomalies in vertebral column, whereas we found no correlation between other variations of intestines (polyps, carcinomas) and anomalies in vertebral columns. It is according to the theory of syndrome anomalies if there is an embryogenesis relation between anomalies of intestines and vertebral column."}
{"id": "PMID:1078271", "title": "[Cholestatic jaundice after taking the laxative Normolaxol].", "content": "Report on two cases of jaundice developing shortly after ingestion of a new laxative, Normolaxol (quinolylmethylenediphenol). Clinical and morphological aspects of the acute cholestasis are described and discussed regarding differential diagnosis and pathogenesis.", "contents": "[Cholestatic jaundice after taking the laxative Normolaxol]. Report on two cases of jaundice developing shortly after ingestion of a new laxative, Normolaxol (quinolylmethylenediphenol). Clinical and morphological aspects of the acute cholestasis are described and discussed regarding differential diagnosis and pathogenesis."}
{"id": "PMID:1078273", "title": "[Radiological study of the neoplastic bladder].", "content": "Radiological methods useful in the examination of bladder tumours are reviewed, with particular reference to preoperative evaluation in accordance with the system. Examples are drawn from a personal series.", "contents": "[Radiological study of the neoplastic bladder]. Radiological methods useful in the examination of bladder tumours are reviewed, with particular reference to preoperative evaluation in accordance with the system. Examples are drawn from a personal series."}
{"id": "PMID:1078274", "title": "[Traumatic lesions of the urinary tract. Radiographic aspects].", "content": "Techniques of radiology of use in the study of traumatic lesions of the urinary apparatus are discussed and 14 cases are reported in detail.", "contents": "[Traumatic lesions of the urinary tract. Radiographic aspects]. Techniques of radiology of use in the study of traumatic lesions of the urinary apparatus are discussed and 14 cases are reported in detail."}
{"id": "PMID:1078275", "title": "[The radiological picture of chronic pyelonephritis].", "content": "The urographical and angiographical signs that aid the radiological diagnosis of the different stages of chronic pyelonephritis are discussed. A number of cases are presented by way of illustration.", "contents": "[The radiological picture of chronic pyelonephritis]. The urographical and angiographical signs that aid the radiological diagnosis of the different stages of chronic pyelonephritis are discussed. A number of cases are presented by way of illustration."}
{"id": "PMID:1078276", "title": "[Results of therapy of carcinoma of the cervix uteri in cases followed from 1962 to 1968 at the Alberti Radio Institute of the Civil Hospitals of Brescia].", "content": "The 5-years follow-up results and late complications in 607 cases of tumour of the uterine cervix, observed at the \"O. Alberti\" Radiotherapy Institute of the Brescia Civil Hospitals between 1962 and 1968 are examined. Treatment used was radiological, surgical or an association of the two.", "contents": "[Results of therapy of carcinoma of the cervix uteri in cases followed from 1962 to 1968 at the Alberti Radio Institute of the Civil Hospitals of Brescia]. The 5-years follow-up results and late complications in 607 cases of tumour of the uterine cervix, observed at the \"O. Alberti\" Radiotherapy Institute of the Brescia Civil Hospitals between 1962 and 1968 are examined. Treatment used was radiological, surgical or an association of the two."}
{"id": "PMID:1078277", "title": "[Complications of pelvic radiotherapy of cervico-uterine carcinoma. Observations on case records from 1954 to 1968].", "content": "Complications observed in 146/674 patients subjected to radiological therapy or radiosurgery at the Modena University Radiology Department between 1954 and 1968 are discussed.", "contents": "[Complications of pelvic radiotherapy of cervico-uterine carcinoma. Observations on case records from 1954 to 1968]. Complications observed in 146/674 patients subjected to radiological therapy or radiosurgery at the Modena University Radiology Department between 1954 and 1968 are discussed."}
{"id": "PMID:1078278", "title": "[Association of cervico-uterine carcinoma and other extra-uterine primary neoplasms. Observations on 31 cases].", "content": "Out of 874 patients undergoing radiotherapy for carcinoma of the uterine cervix, 31 cases (3.5%) of second primary malignant tumour in another site were encountered. Half the cases preceded and half followed uterine cancer. The data are analysed and discussed.", "contents": "[Association of cervico-uterine carcinoma and other extra-uterine primary neoplasms. Observations on 31 cases]. Out of 874 patients undergoing radiotherapy for carcinoma of the uterine cervix, 31 cases (3.5%) of second primary malignant tumour in another site were encountered. Half the cases preceded and half followed uterine cancer. The data are analysed and discussed."}
{"id": "PMID:1078279", "title": "[Clinical and statistical evaluation of radiotherapy of cervico-uterine carcinoma].", "content": "Statistical appraisal of the results of radiotherapy, with or without surgery, in the light of various clinical and technical factors is illustrated with reference to 1466 cases of carcinoma of the cervix treated at the Modena University Radiology Department between 1950 and 1973.", "contents": "[Clinical and statistical evaluation of radiotherapy of cervico-uterine carcinoma]. Statistical appraisal of the results of radiotherapy, with or without surgery, in the light of various clinical and technical factors is illustrated with reference to 1466 cases of carcinoma of the cervix treated at the Modena University Radiology Department between 1950 and 1973."}
{"id": "PMID:1078280", "title": "[Nonvisualized gallbladder in cholecystography per os: radiotomographic study after a second dose].", "content": "Tomography after a second dose of contrast medium gave useful information in 46/57 (81%) cases where the gallbladder was not visualized after a first dose per os. Eight cases are presented in detail.", "contents": "[Nonvisualized gallbladder in cholecystography per os: radiotomographic study after a second dose]. Tomography after a second dose of contrast medium gave useful information in 46/57 (81%) cases where the gallbladder was not visualized after a first dose per os. Eight cases are presented in detail."}
{"id": "PMID:1078281", "title": "[Congenital osteochondrodysplasias. Personal cases].", "content": "The different forms of congenital osteochondrodysplasias are reviewed and personal cases (achondrogenesis; thanatophoric dwarfism; thanatophoric dwarfism associated with cloverleaf skull; asphyxiating thoracic dystrophy etc.) are presented.", "contents": "[Congenital osteochondrodysplasias. Personal cases]. The different forms of congenital osteochondrodysplasias are reviewed and personal cases (achondrogenesis; thanatophoric dwarfism; thanatophoric dwarfism associated with cloverleaf skull; asphyxiating thoracic dystrophy etc.) are presented."}
{"id": "PMID:1078282", "title": "[Angiographic and bronchographic aspects of idiopathic and secondary vanishing lung in childhood].", "content": "Three cases of primary vanishing lung and one case secondary to endobronchial foreign body in children were studied by bronchography and angiography. Results are discussed.", "contents": "[Angiographic and bronchographic aspects of idiopathic and secondary vanishing lung in childhood]. Three cases of primary vanishing lung and one case secondary to endobronchial foreign body in children were studied by bronchography and angiography. Results are discussed."}
{"id": "PMID:1078283", "title": "[2 cases of bronchiectasis caused by an undiagnosed endobronchial foreign body].", "content": "Two cases in which an unknown foreign body gave rise to bronchiectasia are culled from a series of 54 cases of endobronchial foreign bodies in children and discussed.", "contents": "[2 cases of bronchiectasis caused by an undiagnosed endobronchial foreign body]. Two cases in which an unknown foreign body gave rise to bronchiectasia are culled from a series of 54 cases of endobronchial foreign bodies in children and discussed."}
{"id": "PMID:1078284", "title": "[Cockayne syndrome. 3 cases].", "content": "Three cases of Cockayne's syndrome, observed in patients aged 10, 5 and 1 years are reported and discussed; skeletal X-ray findings are illustrated and analysed.", "contents": "[Cockayne syndrome. 3 cases]. Three cases of Cockayne's syndrome, observed in patients aged 10, 5 and 1 years are reported and discussed; skeletal X-ray findings are illustrated and analysed."}
{"id": "PMID:1078285", "title": "[A rare case of external blind accessory urethral canal].", "content": "A case of external, blind accessory urethra in a boy is described. The abnormality was examined radiologically and confirmed surgically.", "contents": "[A rare case of external blind accessory urethral canal]. A case of external, blind accessory urethra in a boy is described. The abnormality was examined radiologically and confirmed surgically."}
{"id": "PMID:1078286", "title": "[Bone localizations of Hodgkin's disease].", "content": "Bone lesions have been observed in 43 out of 415 Hodgkin's disease patients (9.6%): the radiographic findings are discussed with reference to clinical problems.", "contents": "[Bone localizations of Hodgkin's disease]. Bone lesions have been observed in 43 out of 415 Hodgkin's disease patients (9.6%): the radiographic findings are discussed with reference to clinical problems."}
{"id": "PMID:1078287", "title": "[Radiotherapy of Hodgkin's disease: indications, methodology and clinical observations].", "content": "Staging and general management in Hodgkin's disease are reviewed and the radiotherapy technique described. Short-term results with a personal series of 33 cases are reported and early side-effects discussed.", "contents": "[Radiotherapy of Hodgkin's disease: indications, methodology and clinical observations]. Staging and general management in Hodgkin's disease are reviewed and the radiotherapy technique described. Short-term results with a personal series of 33 cases are reported and early side-effects discussed."}
{"id": "PMID:1078288", "title": "[Endocavitary radiotherapy of carcinoma of the cervix uteri using a modeled device and Ir-192. Computerized dosimetry].", "content": "A computerised dosimetry programme for use in intracavitary radiotherapy of cervical carcinoma using a shaped after-loading apparatus and 192Ir wires is described. The technique for its use is explained and the results observed are discussed.", "contents": "[Endocavitary radiotherapy of carcinoma of the cervix uteri using a modeled device and Ir-192. Computerized dosimetry]. A computerised dosimetry programme for use in intracavitary radiotherapy of cervical carcinoma using a shaped after-loading apparatus and 192Ir wires is described. The technique for its use is explained and the results observed are discussed."}
{"id": "PMID:1078289", "title": "[Transillumination of the breast].", "content": "The physical basis of the method, the procedure, the normal and pathological findings are described. On a series of 125 pathology cases observed, transillumination proved useful as a diagnostic aid in breast diseases.", "contents": "[Transillumination of the breast]. The physical basis of the method, the procedure, the normal and pathological findings are described. On a series of 125 pathology cases observed, transillumination proved useful as a diagnostic aid in breast diseases."}
{"id": "PMID:1078290", "title": "[Diagnostic and prognostic value of chest radiography in bed in the acute phases of myocardial infarct].", "content": "The existence of a correlation between Peel's index, pulmonary radiological findings (X-ray carried out in bed immediately after admittance) and clinical condition at discharge has been verified statistically in 92 patients admitted to coronary units for acute myocardial infarction.", "contents": "[Diagnostic and prognostic value of chest radiography in bed in the acute phases of myocardial infarct]. The existence of a correlation between Peel's index, pulmonary radiological findings (X-ray carried out in bed immediately after admittance) and clinical condition at discharge has been verified statistically in 92 patients admitted to coronary units for acute myocardial infarction."}
{"id": "PMID:1078291", "title": "[A simple injector for direct lymphography].", "content": "A gas pressure injector for easy lymphography is described; skilled personnel are not required, cost is very low and operation reliable. The injector has been used without problems in 300 abdominal lymphographies.", "contents": "[A simple injector for direct lymphography]. A gas pressure injector for easy lymphography is described; skilled personnel are not required, cost is very low and operation reliable. The injector has been used without problems in 300 abdominal lymphographies."}
{"id": "PMID:1078293", "title": "[Combined anesthesia with propionylpromazine (Combelen) and methadone (Polamivet). A sure simple method of anesthesia in gastrointestinal surgery in dogs].", "content": "In more than 400 cases of gastrointestinal surgery on mongrel dogs, a combination anesthesia of a neuroplegic agent--propionyl promazine (Combelen) and methadone (Polamivet), an analgesic--was administered without additional intubation or ventilation. This type of anesthesia is simple to apply and practically without risk; the conditions for the operation are therefore excellent. No monitoring personnel is required, no intra- or post-operative incidents have occurred. Thus this method may be recommended as safe and simple for experimental abdominal surgery in dogs.", "contents": "[Combined anesthesia with propionylpromazine (Combelen) and methadone (Polamivet). A sure simple method of anesthesia in gastrointestinal surgery in dogs]. In more than 400 cases of gastrointestinal surgery on mongrel dogs, a combination anesthesia of a neuroplegic agent--propionyl promazine (Combelen) and methadone (Polamivet), an analgesic--was administered without additional intubation or ventilation. This type of anesthesia is simple to apply and practically without risk; the conditions for the operation are therefore excellent. No monitoring personnel is required, no intra- or post-operative incidents have occurred. Thus this method may be recommended as safe and simple for experimental abdominal surgery in dogs."}
{"id": "PMID:1078294", "title": "Internal bile diversion--a new model for the study of cholesterol metabolism.", "content": "Biliary diversion in different ways has been studied as a prevention against induced hypercholesterolemia in experimental animals. A new model for the study of cholesterol metabolism is described in dogs in this paper. The technique consists in ligation and division of the common bile duct and interposition of an isoperistaltic loop of jejunum between the gallbladder and urinary bladder. This technique is free of complications such as diarrhea, infection, and peptic ulceration that are seen in other biliary diversion models, and it is tolerated well by the animals. This requires further study for its effectiveness in the prevention of experimental hypercholesterolemia and subsequent development of arteriosclerosis.", "contents": "Internal bile diversion--a new model for the study of cholesterol metabolism. Biliary diversion in different ways has been studied as a prevention against induced hypercholesterolemia in experimental animals. A new model for the study of cholesterol metabolism is described in dogs in this paper. The technique consists in ligation and division of the common bile duct and interposition of an isoperistaltic loop of jejunum between the gallbladder and urinary bladder. This technique is free of complications such as diarrhea, infection, and peptic ulceration that are seen in other biliary diversion models, and it is tolerated well by the animals. This requires further study for its effectiveness in the prevention of experimental hypercholesterolemia and subsequent development of arteriosclerosis."}
{"id": "PMID:1078292", "title": "[Distribution of dosage and estimation of dosage absorbed by critical organs in the fields of irradiation of lymphomas].", "content": "Dose distribution in blanket irradiation was determined using the photodensitometer in a life-size dummy; doses in critical tissues (especially heart, spinal cord, bone marrow) are discussed with reference to likely biological risks.", "contents": "[Distribution of dosage and estimation of dosage absorbed by critical organs in the fields of irradiation of lymphomas]. Dose distribution in blanket irradiation was determined using the photodensitometer in a life-size dummy; doses in critical tissues (especially heart, spinal cord, bone marrow) are discussed with reference to likely biological risks."}
{"id": "PMID:1078295", "title": "Prerenal hypertension caused by a postoperative A-V fistula.", "content": "The A-V fistula in the renal pedicle with functioning renal parenchyma leads to a prerenal hypertension by the blood-steal effect from the renal artery through the fistula into the vein, according the pressure gradient over the fistula. The etiology of these A-V fistulas is pointed out. A case corrected by surgical removal of the fistula is reported.", "contents": "Prerenal hypertension caused by a postoperative A-V fistula. The A-V fistula in the renal pedicle with functioning renal parenchyma leads to a prerenal hypertension by the blood-steal effect from the renal artery through the fistula into the vein, according the pressure gradient over the fistula. The etiology of these A-V fistulas is pointed out. A case corrected by surgical removal of the fistula is reported."}
{"id": "PMID:1078296", "title": "Lymphangiographic findings in Beh\u00e7et's disease with lymphangiectasia of the small intestine.", "content": "Bipedal lymphography was performed in 5 cases of Beh\u00e7et's disease. In 4 of 5 cases, histologic examination of intestinal biopsy specimens showed lymphatic dilatation of the small intestine which were considered to be a characteristic finding of intestinal lymphangiectasia, a protein-losing enteropathy, but no patient with Beh\u00e7et's disease showed reduced concentration of serum total protein as well as abnormal value of 131I-PVP test. Lymphograms showed an increase in number of iliopelvic and lumbar lymphatic vessels as a major finding, but they failed to demonstrate a hypoplasia or aplasia of lymphatic system or obstruction of thoracic duct. It is conceivable that the lymphatic dilatation of the small intestine in Beh\u00e7et's disease may be related to increased flow of lymph due to excessive vascular hyperpermeability and may not be related to a block of lymphatic system which has been considered to be a cause of enteric protein loss in intestinal lymphangiectasia. The authors wish to emphasize that the dilatation of lymphatic vessel of the small intestine is not a sufficient finding to indicate the presence of protein-losing enteropathy.", "contents": "Lymphangiographic findings in Beh\u00e7et's disease with lymphangiectasia of the small intestine. Bipedal lymphography was performed in 5 cases of Beh\u00e7et's disease. In 4 of 5 cases, histologic examination of intestinal biopsy specimens showed lymphatic dilatation of the small intestine which were considered to be a characteristic finding of intestinal lymphangiectasia, a protein-losing enteropathy, but no patient with Beh\u00e7et's disease showed reduced concentration of serum total protein as well as abnormal value of 131I-PVP test. Lymphograms showed an increase in number of iliopelvic and lumbar lymphatic vessels as a major finding, but they failed to demonstrate a hypoplasia or aplasia of lymphatic system or obstruction of thoracic duct. It is conceivable that the lymphatic dilatation of the small intestine in Beh\u00e7et's disease may be related to increased flow of lymph due to excessive vascular hyperpermeability and may not be related to a block of lymphatic system which has been considered to be a cause of enteric protein loss in intestinal lymphangiectasia. The authors wish to emphasize that the dilatation of lymphatic vessel of the small intestine is not a sufficient finding to indicate the presence of protein-losing enteropathy."}
{"id": "PMID:1078297", "title": "Ventricular activity during atrial fibrillation. Studies with His bundle electrography.", "content": "His bundle electrograms were recorded in 12 patients with atrial fibrillation. In all cases but one, the dominant rhythm was atrial fibrillation. In 1 case the dominant rhythm was fascicular tachycardia due probably to digitalis overdosage. However ventricular, fascicular and junctional beats could be often recognized on His bundle tracings, even when QRS configuration was similar to supraventricular conducted beats due to atrial fibrillation. In the patients in whom sinus rhythm occurred, the H-V interval was unchanged. Although it is admitted that during atrial fibrillation the zone of concealment of A-V conduction is in the A-V nodal area, concealed penetration of atrial impulses in the fascicular system may be encountered in patients not treated with digitalis.", "contents": "Ventricular activity during atrial fibrillation. Studies with His bundle electrography. His bundle electrograms were recorded in 12 patients with atrial fibrillation. In all cases but one, the dominant rhythm was atrial fibrillation. In 1 case the dominant rhythm was fascicular tachycardia due probably to digitalis overdosage. However ventricular, fascicular and junctional beats could be often recognized on His bundle tracings, even when QRS configuration was similar to supraventricular conducted beats due to atrial fibrillation. In the patients in whom sinus rhythm occurred, the H-V interval was unchanged. Although it is admitted that during atrial fibrillation the zone of concealment of A-V conduction is in the A-V nodal area, concealed penetration of atrial impulses in the fascicular system may be encountered in patients not treated with digitalis."}
{"id": "PMID:1078299", "title": "Serum iron, glutamic oxalacetic transaminase and creatine phosphokinase in acute myocardial infarction. A comparative study.", "content": "The serum iron was subnormal on the first three days, following acute myocardial infarction in thirty patients. This is significantly less than the incidence of abnormalities demonstrated by assays of creatine phosphokinase (CPK) and glutamic oxalacetic transaminase (SGOT) in serum. Occasional patients had abnormal serum iron concentrations in association with normal CPK activities. For this reason serum iron determination may usefully supplement CPK activity measurements and possibly those of SGOT activity as an aid to the detection of acute myocardial infarction.", "contents": "Serum iron, glutamic oxalacetic transaminase and creatine phosphokinase in acute myocardial infarction. A comparative study. The serum iron was subnormal on the first three days, following acute myocardial infarction in thirty patients. This is significantly less than the incidence of abnormalities demonstrated by assays of creatine phosphokinase (CPK) and glutamic oxalacetic transaminase (SGOT) in serum. Occasional patients had abnormal serum iron concentrations in association with normal CPK activities. For this reason serum iron determination may usefully supplement CPK activity measurements and possibly those of SGOT activity as an aid to the detection of acute myocardial infarction."}
{"id": "PMID:1078300", "title": "Surgical treatment of the congenital fistulae of coronary arteries.", "content": "Six cases of coronary artery fistulae surgically treated are presented. All patient but one was male; the ages varied from 4 to 44 years. The fistulae originated from the left coronary artery in three cases from the right in two cases and from both arteries in one case. The draining heart chambers were the right atrium in three, the right ventricle in two and the pulmonary artery in one case. Associated lesions were present in three cases. One patient died on the early postoperative period and the evolution was satisfactory in five cases.", "contents": "Surgical treatment of the congenital fistulae of coronary arteries. Six cases of coronary artery fistulae surgically treated are presented. All patient but one was male; the ages varied from 4 to 44 years. The fistulae originated from the left coronary artery in three cases from the right in two cases and from both arteries in one case. The draining heart chambers were the right atrium in three, the right ventricle in two and the pulmonary artery in one case. Associated lesions were present in three cases. One patient died on the early postoperative period and the evolution was satisfactory in five cases."}
{"id": "PMID:1078301", "title": "Venous pressure and compliance of the peripheral capacitance vessels.", "content": "Compliances of the peripheral capacitance vessels decreased gradually in accordance with the increase in pressure of the brachial vein. The relationship between them formed a number of hyperbolic-like curves, the same results as those found in many cases of both normal subjects and patients with congestive heart failure. The decrease of compliance of the peripheral capacitance vessels was not the result of an organic change, but of a functional change of the vessels caused by distention.", "contents": "Venous pressure and compliance of the peripheral capacitance vessels. Compliances of the peripheral capacitance vessels decreased gradually in accordance with the increase in pressure of the brachial vein. The relationship between them formed a number of hyperbolic-like curves, the same results as those found in many cases of both normal subjects and patients with congestive heart failure. The decrease of compliance of the peripheral capacitance vessels was not the result of an organic change, but of a functional change of the vessels caused by distention."}
{"id": "PMID:1078303", "title": "Traumatic lateral sinus thrombosis. Report of two cases.", "content": "Two cases with thrombosis of the transverse sinus caused by a fracture crossing the sinus are reported, and the roentgenological changes seen in cerebral angiography are discussed. In 1 case, a filling defect was seen which at subsequent autopsy was found to correspond with an intraluminal thrombosis. In the other case, not verified at autopsy, the angiographic changes were consistent with a thrombosis of a transverse sinus.", "contents": "Traumatic lateral sinus thrombosis. Report of two cases. Two cases with thrombosis of the transverse sinus caused by a fracture crossing the sinus are reported, and the roentgenological changes seen in cerebral angiography are discussed. In 1 case, a filling defect was seen which at subsequent autopsy was found to correspond with an intraluminal thrombosis. In the other case, not verified at autopsy, the angiographic changes were consistent with a thrombosis of a transverse sinus."}
{"id": "PMID:1078304", "title": "Compliance of the peripheral capacitance vessels in patients with congestive heart failure.", "content": "A method for measuring compliance of the peripheral capacitance vessels is reported, and the values of compliance in patients with congestive heart failure are compared with those in normal subjects. Peripheral venous compliance and right brachial venous pressure in both patients with congestive heart failure and in normal subjects, when plotted graphically, showed a relationship described by many hyperbolic curves. The mean value of compliance of the peripheral capacitance vessels in patients with congestive heart failure was only half that in normal subjects. This difference was statistically significant (P less than 0.001). After the congestion in the patients with congestive heart failure disappeared following treatment with digitalis and other drugs, the values of compliance became nearly the same as in normal subjects. It seems that the increase of compliance of the peripheral capacitance vessels is not due to an organic change but to a functional change of the vessels caused by distention.", "contents": "Compliance of the peripheral capacitance vessels in patients with congestive heart failure. A method for measuring compliance of the peripheral capacitance vessels is reported, and the values of compliance in patients with congestive heart failure are compared with those in normal subjects. Peripheral venous compliance and right brachial venous pressure in both patients with congestive heart failure and in normal subjects, when plotted graphically, showed a relationship described by many hyperbolic curves. The mean value of compliance of the peripheral capacitance vessels in patients with congestive heart failure was only half that in normal subjects. This difference was statistically significant (P less than 0.001). After the congestion in the patients with congestive heart failure disappeared following treatment with digitalis and other drugs, the values of compliance became nearly the same as in normal subjects. It seems that the increase of compliance of the peripheral capacitance vessels is not due to an organic change but to a functional change of the vessels caused by distention."}
{"id": "PMID:1078305", "title": "Effects of diuretics on lipid metabolism in patients with essential hypertension.", "content": "Available clinical evidence indicates a high prevalence of hyperuricemia in patients with essential hypertension; this becomes accentuated with diuretic therapy. Since there is an association of hyperlipidemia with hyperuricuria and hypertension and since hyperuricemia is a feature of diuretic therapy, we explored whether these relationships might be provoked by prolonged diuretic therapy. Eighteen male patients with uncomplicated essential hypertension of mild severity were treated for 9 months with hydrochlorothiazide and supplemental potassium chloride, 100 mg and 45 mEq/day, respectively. Arterial pressure, renal function, and serum electrolyte, uric acid, blood glucose, and lipid concentrations were measured several times before and during therapy. Arterial pressure remained significantly reduced during therapy (P less than 0.001); this was associated with reduced serum potassium (P less than 0.01) and increased blood glucose and serum uric acid concentrations (P less than 0.005, P less than .025, respectively). Blood urea nitrogen, serum creatinine, sodium, cholesterol and triglyceride levels did not significantly change with treatment. Thus, although diuretics increase serum uric acid and blood glucose, their effect on serum lipid concentration is negligible.", "contents": "Effects of diuretics on lipid metabolism in patients with essential hypertension. Available clinical evidence indicates a high prevalence of hyperuricemia in patients with essential hypertension; this becomes accentuated with diuretic therapy. Since there is an association of hyperlipidemia with hyperuricuria and hypertension and since hyperuricemia is a feature of diuretic therapy, we explored whether these relationships might be provoked by prolonged diuretic therapy. Eighteen male patients with uncomplicated essential hypertension of mild severity were treated for 9 months with hydrochlorothiazide and supplemental potassium chloride, 100 mg and 45 mEq/day, respectively. Arterial pressure, renal function, and serum electrolyte, uric acid, blood glucose, and lipid concentrations were measured several times before and during therapy. Arterial pressure remained significantly reduced during therapy (P less than 0.001); this was associated with reduced serum potassium (P less than 0.01) and increased blood glucose and serum uric acid concentrations (P less than 0.005, P less than .025, respectively). Blood urea nitrogen, serum creatinine, sodium, cholesterol and triglyceride levels did not significantly change with treatment. Thus, although diuretics increase serum uric acid and blood glucose, their effect on serum lipid concentration is negligible."}
{"id": "PMID:1078307", "title": "Venous thrombosis in childhood. A case report and review of the literature.", "content": "Venous thrombosis in childhood is an extremely rare condition. The etiologic factors in childhood are different than in the adult. Generalized infection seems to play the main etiologic role, and for this reason antibiotic treatment is usually necessary. Anticoagulant treatment is mandatory; however its application presents many difficulties. The administration of calcium-heparin subcutaneously for a long time is feasible, well-controlled, without side effects and highly effective. The authors therefore strongly recommended this anticoagulant treatment in childhood.", "contents": "Venous thrombosis in childhood. A case report and review of the literature. Venous thrombosis in childhood is an extremely rare condition. The etiologic factors in childhood are different than in the adult. Generalized infection seems to play the main etiologic role, and for this reason antibiotic treatment is usually necessary. Anticoagulant treatment is mandatory; however its application presents many difficulties. The administration of calcium-heparin subcutaneously for a long time is feasible, well-controlled, without side effects and highly effective. The authors therefore strongly recommended this anticoagulant treatment in childhood."}
{"id": "PMID:1078308", "title": "Sudden death and the floppy mitral valve syndrome.", "content": "A 38-year-old female with a history of palpitations, premature ventricular contractions, and a systolic murmur collapsed suddenly and died. Post-mortem studies showed shortened chordae tendinae and myxomatous transformation of both mitral leaflets. As this syndrome becomes more clinically recognizable it appears that its benignancy is diminishing. Thus, it is felt that patients with findings of ventricular extrasystoles and a systolic murmur should have a careful cardiac evaluation to determine the presence of the floppy mitral valve or ballooning mitral leaflet syndrome and that appropriate measures for treatment be taken when necessary.", "contents": "Sudden death and the floppy mitral valve syndrome. A 38-year-old female with a history of palpitations, premature ventricular contractions, and a systolic murmur collapsed suddenly and died. Post-mortem studies showed shortened chordae tendinae and myxomatous transformation of both mitral leaflets. As this syndrome becomes more clinically recognizable it appears that its benignancy is diminishing. Thus, it is felt that patients with findings of ventricular extrasystoles and a systolic murmur should have a careful cardiac evaluation to determine the presence of the floppy mitral valve or ballooning mitral leaflet syndrome and that appropriate measures for treatment be taken when necessary."}
{"id": "PMID:1078310", "title": "Multiple lead ECG submaximal treadmill exercise tests in angiographically documented coronary heart disease.", "content": "One hundred patients underwent submaximal exercise tolerance testing and coronary cineangiography. Sixty-six percent of the exercise tests were diagnostic including 48 positive and 18 negative tests; 34 patients had indeterminate test results. The occurrence of exercise induced ventricular premature beats was not related to significant coronary artery disease. Ventricular asynergy was significantly more frequent in patients with positive exercise tests (p less than .0001). Application of the age-adjusted target heart rate criterion recommended from Scandinavia and Myrtle Beach to patients with indeterminate results due to failure to reach target heart rate resulted in six false negative tests and lowered sensitivity. The number of positive diagnostic responses achieved using a multiple electrocardiographic lead system was compared with positive diagnostic responses detected in a single lead (V5) and the number of positive tests identified by the additional leads was highly significant (p less than .0001). A high incidence of indeterminate test results due to failure to achieve target heart rate is noted.", "contents": "Multiple lead ECG submaximal treadmill exercise tests in angiographically documented coronary heart disease. One hundred patients underwent submaximal exercise tolerance testing and coronary cineangiography. Sixty-six percent of the exercise tests were diagnostic including 48 positive and 18 negative tests; 34 patients had indeterminate test results. The occurrence of exercise induced ventricular premature beats was not related to significant coronary artery disease. Ventricular asynergy was significantly more frequent in patients with positive exercise tests (p less than .0001). Application of the age-adjusted target heart rate criterion recommended from Scandinavia and Myrtle Beach to patients with indeterminate results due to failure to reach target heart rate resulted in six false negative tests and lowered sensitivity. The number of positive diagnostic responses achieved using a multiple electrocardiographic lead system was compared with positive diagnostic responses detected in a single lead (V5) and the number of positive tests identified by the additional leads was highly significant (p less than .0001). A high incidence of indeterminate test results due to failure to achieve target heart rate is noted."}
{"id": "PMID:1078311", "title": "Traumatic arteriovenous fistulas. A follow-up study.", "content": "Over the period 1958 to 1972 14 patients with traumatic arteriovenous fistulas were treated. The mean period from trauma to surgical closure of the fistula was 13.3 years. No form of reconstructive vascular surgery had been performed initially. The dominating symptom of arteriovenous fistula was a pulsating tumor with a marked thrill. Excision of the fistula and reconstruction of the artery and vein was performed in 11 patients. In 3 patients the fistula was first ligated resulting in early recurrence of the shunt. At follow-up 3-15 years postoperatively all fistulas were closed, but 5 patients had moderate symptoms.", "contents": "Traumatic arteriovenous fistulas. A follow-up study. Over the period 1958 to 1972 14 patients with traumatic arteriovenous fistulas were treated. The mean period from trauma to surgical closure of the fistula was 13.3 years. No form of reconstructive vascular surgery had been performed initially. The dominating symptom of arteriovenous fistula was a pulsating tumor with a marked thrill. Excision of the fistula and reconstruction of the artery and vein was performed in 11 patients. In 3 patients the fistula was first ligated resulting in early recurrence of the shunt. At follow-up 3-15 years postoperatively all fistulas were closed, but 5 patients had moderate symptoms."}
{"id": "PMID:1078312", "title": "Pattern of occlusive peripheral vascular disease in India. (clinicopathological study of 79 cases).", "content": "The pattern of peripheral vascular disease in India was studied in 89 patients undergoing amputation for vascular insufficiency. A control group of 26 limbs was studied for aging changes. Clinically, cases were classified into TAO (25), ASO (54) and thrombo-embolic disease (10). Angiographic, macroscopic and microscopic study of dissected vascular tree was done in view of clinical diagnosis. No significant difference was noted in the frequency and extent of involvement of limb arteries in TAO and ASO. Histologically angiitis was noted in 4 cases of TAO and one of ASO but these did not form a distinct clinical group. Primary thrombotic occlusion was seen in 21 cases of TAO and 23 cases of ASO. Atheroma with and without thrombosis was found in 26 cases. In 4 cases the arterial tree was normal. Atheromatous lesions were not seen in control group. In the groups under study they were thought to represent old thrombi rather than degenerative lesions predisposing to thrombosis. In view of these findings it was felt that Buerger's disease was primarily a thrombotic disorder. Angiitis was rare and was probably secondary. The two clinical groups TAO and ASO are not pathologically distinct as very high percentage of the latter showed primary thrombosis. The genesis of thrombosis in two groups however may be different.", "contents": "Pattern of occlusive peripheral vascular disease in India. (clinicopathological study of 79 cases). The pattern of peripheral vascular disease in India was studied in 89 patients undergoing amputation for vascular insufficiency. A control group of 26 limbs was studied for aging changes. Clinically, cases were classified into TAO (25), ASO (54) and thrombo-embolic disease (10). Angiographic, macroscopic and microscopic study of dissected vascular tree was done in view of clinical diagnosis. No significant difference was noted in the frequency and extent of involvement of limb arteries in TAO and ASO. Histologically angiitis was noted in 4 cases of TAO and one of ASO but these did not form a distinct clinical group. Primary thrombotic occlusion was seen in 21 cases of TAO and 23 cases of ASO. Atheroma with and without thrombosis was found in 26 cases. In 4 cases the arterial tree was normal. Atheromatous lesions were not seen in control group. In the groups under study they were thought to represent old thrombi rather than degenerative lesions predisposing to thrombosis. In view of these findings it was felt that Buerger's disease was primarily a thrombotic disorder. Angiitis was rare and was probably secondary. The two clinical groups TAO and ASO are not pathologically distinct as very high percentage of the latter showed primary thrombosis. The genesis of thrombosis in two groups however may be different."}
{"id": "PMID:1078314", "title": "Improvement of the flow properties of blood: a new therapeutical approach in occlusive arterial disease.", "content": "The general pathophysiological basis in occlusive arterial disease is the reduced flow rate of blood in the microcirculation. Blood flow in the ischemic tissue can be increased by improving the flow properties of blood. The fibrinogen concentration of blood and the deformability of red cells are two main factors determining the flow properties of blood. Ancrod, a fibrinogen-lowering substance, improves the flow properties of blood by decreasing the viscosity of blood and plasma and by desaggregation of erythrocyte aggregates. Treatment of patients suffering from severe occlusive arterial diseases with Ancrod exhibits surprisingly good results. Disappearance of rest pains and reduction in the use of analgetic drugs has been proved statistically. In the exercising muscles a local hyperosmolarity exists, which is able to decrease the deformability of red cells thereby impairing the flow in narrow capillaries. The addition of Pentoxifyllin to blood in-vitro reduces the rigidity of red cells in hyperosmolar blood samples by increasing their cellular ATP-content. Thus the reduced flow rate of hyperosmolar blood through 8 mu-capillaries could be increased twice (p less than 0.0005) by the addition of Pentoxifyllin. These in-vitro results could be the starting point of a new idea for the treatment of patients with intermittent claudication.", "contents": "Improvement of the flow properties of blood: a new therapeutical approach in occlusive arterial disease. The general pathophysiological basis in occlusive arterial disease is the reduced flow rate of blood in the microcirculation. Blood flow in the ischemic tissue can be increased by improving the flow properties of blood. The fibrinogen concentration of blood and the deformability of red cells are two main factors determining the flow properties of blood. Ancrod, a fibrinogen-lowering substance, improves the flow properties of blood by decreasing the viscosity of blood and plasma and by desaggregation of erythrocyte aggregates. Treatment of patients suffering from severe occlusive arterial diseases with Ancrod exhibits surprisingly good results. Disappearance of rest pains and reduction in the use of analgetic drugs has been proved statistically. In the exercising muscles a local hyperosmolarity exists, which is able to decrease the deformability of red cells thereby impairing the flow in narrow capillaries. The addition of Pentoxifyllin to blood in-vitro reduces the rigidity of red cells in hyperosmolar blood samples by increasing their cellular ATP-content. Thus the reduced flow rate of hyperosmolar blood through 8 mu-capillaries could be increased twice (p less than 0.0005) by the addition of Pentoxifyllin. These in-vitro results could be the starting point of a new idea for the treatment of patients with intermittent claudication."}
{"id": "PMID:1078315", "title": "Phonocephalography extra-cephalic.", "content": "Phonocephalography is the amplification and recording of sounds from the surface and cavities of the head aiming for the introduction of a non-invasive pre-angiographic technique. Nasopharyngeal Phonocephalography was found successful for recording of normal tracing. The effect of the extra-cephalic sounds and factors on the tracing were discussed. Phonomyelography is the application of the idea on the vertebral column. The tracing recorded was demonstrated. Modern technology has undergone considerable progress for the introduction of non-invasive techniques in medical diagnosis. Since Laennec introduced his stethoscope auscultation, amplification and recording of sounds in cardiology had reached a respectable diagnostic procedure. This article represents a trial for the introduction of a simple non-invasive pre-angiographic diagnostic technique by amplification and recording of sounds from the surface and cavities of the body.", "contents": "Phonocephalography extra-cephalic. Phonocephalography is the amplification and recording of sounds from the surface and cavities of the head aiming for the introduction of a non-invasive pre-angiographic technique. Nasopharyngeal Phonocephalography was found successful for recording of normal tracing. The effect of the extra-cephalic sounds and factors on the tracing were discussed. Phonomyelography is the application of the idea on the vertebral column. The tracing recorded was demonstrated. Modern technology has undergone considerable progress for the introduction of non-invasive techniques in medical diagnosis. Since Laennec introduced his stethoscope auscultation, amplification and recording of sounds in cardiology had reached a respectable diagnostic procedure. This article represents a trial for the introduction of a simple non-invasive pre-angiographic diagnostic technique by amplification and recording of sounds from the surface and cavities of the body."}
{"id": "PMID:1078317", "title": "A clinical approach to hypertension.", "content": "1. A new approach to hypertension is introduced by taking into consideration not only casual blood pressure but also baseline blood pressure, blood pressure lability and cold pressor response. 2. In addition to blood pressure measurements the vascular damage in terms of atherosclerosis and/or retinal arteriosclerosis was also taken into consideration by grouping the patients into four groups: Normal; Arteriosclerosis; Hypertension; Hypertension & superimposed arteriosclerosis. 3. Prior to initiating treatment, at least six casual blood pressures were taken by the technical assistant and the physician, during three different visits. 4. The step method, by starting with thiazides and adding other antihypertensive agents during the subsequent office visits according to response was followed. 5. The patients were followed for a four to seven year period so that a meaningful prognostic and therapeutic evaluation was possible. 6. All patients with pure hypertension and without arteriosclerosis and those with pure arteriosclerosis and without hypertension survived during the period of observation. 7. Twenty-three per cent (12 of 51) of males having hypertension with super-imposed arteriosclerosis died, as compared to only 4% (2 of 54) of females; thus a male to female mortality ratio of about 6:1 was observed. 8. A comparison of the living and deceased males of the mixed group having hypertension and superimposed arteriosclerosis showed that the most significant difference between the two subgroups was a marked exaggeration of diastolic cold pressor response in the deceased as compared to the still living patients. 9. It remains to be seen whether or not in these patients with a marked exaggeration of the diastolic cold pressor response, by using a more vigorous treatment with drugs such as methyldopa, propranolol or guanethidine in relatively high doses, we might be able to reduce mortality and increase survival.", "contents": "A clinical approach to hypertension. 1. A new approach to hypertension is introduced by taking into consideration not only casual blood pressure but also baseline blood pressure, blood pressure lability and cold pressor response. 2. In addition to blood pressure measurements the vascular damage in terms of atherosclerosis and/or retinal arteriosclerosis was also taken into consideration by grouping the patients into four groups: Normal; Arteriosclerosis; Hypertension; Hypertension & superimposed arteriosclerosis. 3. Prior to initiating treatment, at least six casual blood pressures were taken by the technical assistant and the physician, during three different visits. 4. The step method, by starting with thiazides and adding other antihypertensive agents during the subsequent office visits according to response was followed. 5. The patients were followed for a four to seven year period so that a meaningful prognostic and therapeutic evaluation was possible. 6. All patients with pure hypertension and without arteriosclerosis and those with pure arteriosclerosis and without hypertension survived during the period of observation. 7. Twenty-three per cent (12 of 51) of males having hypertension with super-imposed arteriosclerosis died, as compared to only 4% (2 of 54) of females; thus a male to female mortality ratio of about 6:1 was observed. 8. A comparison of the living and deceased males of the mixed group having hypertension and superimposed arteriosclerosis showed that the most significant difference between the two subgroups was a marked exaggeration of diastolic cold pressor response in the deceased as compared to the still living patients. 9. It remains to be seen whether or not in these patients with a marked exaggeration of the diastolic cold pressor response, by using a more vigorous treatment with drugs such as methyldopa, propranolol or guanethidine in relatively high doses, we might be able to reduce mortality and increase survival."}
{"id": "PMID:1078318", "title": "Stabilized venous distensibility of normotensive and hypertensive humans on high and low sodium intake.", "content": "Venous responses to stabilized orthostasis (45 degrees head-up tilt) were studied in seven normotensive subjects and eight hypertensive patients, when on high and low dietary sodium intake. Exchangeable sodium and blood volumes were determined to permit correlation with any significant changes in venous behavior. The intent of this study was to detect and analyze any diet-induced changes in responses of forearm veins to prolonged orthostasis. The pharmacological effects of sodium depletion by medication and diet on arteries and veins of hypertensives are discussed. The results of this study indicate that dietary sodium depletion did not have adverse effects on the ability to maintain stabilized venous tone during orthostasis. These results support recommendations that moderate dietary sodium restriction be included as part of antihypertensive regimens.", "contents": "Stabilized venous distensibility of normotensive and hypertensive humans on high and low sodium intake. Venous responses to stabilized orthostasis (45 degrees head-up tilt) were studied in seven normotensive subjects and eight hypertensive patients, when on high and low dietary sodium intake. Exchangeable sodium and blood volumes were determined to permit correlation with any significant changes in venous behavior. The intent of this study was to detect and analyze any diet-induced changes in responses of forearm veins to prolonged orthostasis. The pharmacological effects of sodium depletion by medication and diet on arteries and veins of hypertensives are discussed. The results of this study indicate that dietary sodium depletion did not have adverse effects on the ability to maintain stabilized venous tone during orthostasis. These results support recommendations that moderate dietary sodium restriction be included as part of antihypertensive regimens."}
{"id": "PMID:1078320", "title": "Systolic time intervals and heart sounds in left bundle branch block.", "content": "Systolic time intervals and heart sounds were studied in twenty patients with left bundle branch block. A definite cause for the organic heart disease was not ascertained. Abnormal duration of the electromechanical systole (Q-A2), due to a prolongation both of the preejection period (PEP) and of the Q-M1 intervals, was found to exist in all. Prolongation of Q-M1 was due to lengthening either of the electromechanical interval (EMI) or of the pre-isovolumetric contraction time (PICT). Isovolumetric contraction time (ICT) was prolonged in thirteen patients only. There was a good correlation between LVET/ICT ratio and the duration of ICT intervals. Six patients showed a first heart sound of normal intensity and duration. There was no correlation between the duration of the systolic time intervals and the characteristics of the first and second heart sounds.", "contents": "Systolic time intervals and heart sounds in left bundle branch block. Systolic time intervals and heart sounds were studied in twenty patients with left bundle branch block. A definite cause for the organic heart disease was not ascertained. Abnormal duration of the electromechanical systole (Q-A2), due to a prolongation both of the preejection period (PEP) and of the Q-M1 intervals, was found to exist in all. Prolongation of Q-M1 was due to lengthening either of the electromechanical interval (EMI) or of the pre-isovolumetric contraction time (PICT). Isovolumetric contraction time (ICT) was prolonged in thirteen patients only. There was a good correlation between LVET/ICT ratio and the duration of ICT intervals. Six patients showed a first heart sound of normal intensity and duration. There was no correlation between the duration of the systolic time intervals and the characteristics of the first and second heart sounds."}
{"id": "PMID:1078321", "title": "Prolonged atrial standstill.", "content": "A 55 year old man with hypertensive cardiovascular disease and uremia was studied. Atrial standstill was confirmed by intracardiac electrocardiography and by inability to stimulate the atria using intraatrial cardiac electrical pacing. Atrial standstill was observed to persist for at least three months in duration. This rare cardiac mechanism disorder of atrial standstill, its characteristics and possible mechanisms are reviewed.", "contents": "Prolonged atrial standstill. A 55 year old man with hypertensive cardiovascular disease and uremia was studied. Atrial standstill was confirmed by intracardiac electrocardiography and by inability to stimulate the atria using intraatrial cardiac electrical pacing. Atrial standstill was observed to persist for at least three months in duration. This rare cardiac mechanism disorder of atrial standstill, its characteristics and possible mechanisms are reviewed."}
{"id": "PMID:1078322", "title": "Retrograde catheterization of the pulmonary veins following surgical correction of transposition of the great vessels.", "content": "Prior to a Mustard repair for transposition of the great vessels, complete physiologic data of the pulmonary bed can be obtained by catheterization of the pulmonary artery with a \"J\" formed wire, and direct catheterization of the pulmonary veins via an atrial septal defect. Following \"physiologic\" repair ready access to the pulmonary veins is denied. Evaluation of the pulmonary venous pressure is of great importance, however, in differentiating pulmonary vascular disease from pulmonary hypertension secondary to patch obstruction of pulmonary venous return. This paper describes a technique of catheterization of the pulmonary veins following a Mustard repair for TGV.", "contents": "Retrograde catheterization of the pulmonary veins following surgical correction of transposition of the great vessels. Prior to a Mustard repair for transposition of the great vessels, complete physiologic data of the pulmonary bed can be obtained by catheterization of the pulmonary artery with a \"J\" formed wire, and direct catheterization of the pulmonary veins via an atrial septal defect. Following \"physiologic\" repair ready access to the pulmonary veins is denied. Evaluation of the pulmonary venous pressure is of great importance, however, in differentiating pulmonary vascular disease from pulmonary hypertension secondary to patch obstruction of pulmonary venous return. This paper describes a technique of catheterization of the pulmonary veins following a Mustard repair for TGV."}
{"id": "PMID:1078325", "title": "Studies of hemorrhage on the optic disc.", "content": "The general medical and laboratory findings in 60 patients having a sector hemorrhage on the optic disc were compared with the findings in 102 patients with intraocular pressure elevation but without a disc hemorrhage. Of 22 parameters studied, systemic hypertension was the only abnormality which occurred significantly more frequently in the hemorrhage group. A study of the intraocular pressures suggests that hemorrhage may not be related to the level of intraocular pressure. Examinations of 50 patients with systemic hypertension and of 50 patients with cardiac abnormalities revealed no hemorrhage on the optic disc. These factors by themselves may not be responsible for the occurrence of hemorrhages.", "contents": "Studies of hemorrhage on the optic disc. The general medical and laboratory findings in 60 patients having a sector hemorrhage on the optic disc were compared with the findings in 102 patients with intraocular pressure elevation but without a disc hemorrhage. Of 22 parameters studied, systemic hypertension was the only abnormality which occurred significantly more frequently in the hemorrhage group. A study of the intraocular pressures suggests that hemorrhage may not be related to the level of intraocular pressure. Examinations of 50 patients with systemic hypertension and of 50 patients with cardiac abnormalities revealed no hemorrhage on the optic disc. These factors by themselves may not be responsible for the occurrence of hemorrhages."}
{"id": "PMID:1078323", "title": "The influence of cycle length on the effective and functional refractory period of the human AV node.", "content": "The influence of atrial cycle length on the effective and functional refractory period of the human AV node and atrium is presented. In general, decrements in atrial cycle lengths resulted in a shortening of the refractory period of the atrium and the functional refractory period of the AV node. The effective refractory period of the AV node was not significantly prolonged. The physiologic significance of the effective and functional refractory periods of the AV node are discussed.", "contents": "The influence of cycle length on the effective and functional refractory period of the human AV node. The influence of atrial cycle length on the effective and functional refractory period of the human AV node and atrium is presented. In general, decrements in atrial cycle lengths resulted in a shortening of the refractory period of the atrium and the functional refractory period of the AV node. The effective refractory period of the AV node was not significantly prolonged. The physiologic significance of the effective and functional refractory periods of the AV node are discussed."}
{"id": "PMID:1078326", "title": "Invasion of the optic nerve by retinoblastoma: a clinicopathological study.", "content": "We have studied all cases of optic nerve invasion in retinoblastoma seen at the Edward S. Harkness Eye Institute in New York since 1925. Since 1959 optic nerve invasion has been less extensive--suggesting earlier diagnosis and management. Since 1959 there has also been an increase in the number surviving. Survival correlates with the extent of spread to optic nerve, choroid and extrabulbar tissues. Current and future therapy are discussed. The importance of subpial extension of retinoblastoma is noted and its relationship to the mode of therapy is emphasized.", "contents": "Invasion of the optic nerve by retinoblastoma: a clinicopathological study. We have studied all cases of optic nerve invasion in retinoblastoma seen at the Edward S. Harkness Eye Institute in New York since 1925. Since 1959 optic nerve invasion has been less extensive--suggesting earlier diagnosis and management. Since 1959 there has also been an increase in the number surviving. Survival correlates with the extent of spread to optic nerve, choroid and extrabulbar tissues. Current and future therapy are discussed. The importance of subpial extension of retinoblastoma is noted and its relationship to the mode of therapy is emphasized."}
{"id": "PMID:1078327", "title": "Complications of corticosteroid therapy in presumptive temporal arteritis.", "content": "Two elderly patients presented with symptoms suggestive of occult temporal arteritis. Both were treated with high doses of corticosteroids although subsequent biopsies of the arteries did not show evidence of arteritis. After 5 months of corticosteroid therapy, the first patient died of Gram-negative bacterial pneumonia and cystitis and disseminated intravascular coagulation. The second patient, six days after the biopsy, died of pneumococcal meningitis which had presumably spread from a focus about the left optic nerve. In the first patient, necropsy studies showed that the loss of vision appeared to be due to arteriosclerosis of the nutrient vessels of the optic nerve while in the second patient, the visual symptoms appeared to be due to a localized optic perineuritis. Corticosteroid therapy in elderly patients carries a high morbidity, as is illustrated by the first case and may mask unsuspected underlying disease processes, as presumably occurred in the second. We discuss the importance of obtaining a biopsy diagnostic of temporal arteritis in order to justify the continuation of corticosteroid therapy and the significance of a negative biopsy.", "contents": "Complications of corticosteroid therapy in presumptive temporal arteritis. Two elderly patients presented with symptoms suggestive of occult temporal arteritis. Both were treated with high doses of corticosteroids although subsequent biopsies of the arteries did not show evidence of arteritis. After 5 months of corticosteroid therapy, the first patient died of Gram-negative bacterial pneumonia and cystitis and disseminated intravascular coagulation. The second patient, six days after the biopsy, died of pneumococcal meningitis which had presumably spread from a focus about the left optic nerve. In the first patient, necropsy studies showed that the loss of vision appeared to be due to arteriosclerosis of the nutrient vessels of the optic nerve while in the second patient, the visual symptoms appeared to be due to a localized optic perineuritis. Corticosteroid therapy in elderly patients carries a high morbidity, as is illustrated by the first case and may mask unsuspected underlying disease processes, as presumably occurred in the second. We discuss the importance of obtaining a biopsy diagnostic of temporal arteritis in order to justify the continuation of corticosteroid therapy and the significance of a negative biopsy."}
{"id": "PMID:1078328", "title": "Sympathetic ophthalmitis. Successful treatment of late cases.", "content": "Three severe cases of sympathetic ophthalmitis, which presented late for treatment, are described. The response to prolonged treatment with systemic prednisolone was unexpectedly good, occurring as long as 70 days after starting treatment and continuing up to 16 months.", "contents": "Sympathetic ophthalmitis. Successful treatment of late cases. Three severe cases of sympathetic ophthalmitis, which presented late for treatment, are described. The response to prolonged treatment with systemic prednisolone was unexpectedly good, occurring as long as 70 days after starting treatment and continuing up to 16 months."}
{"id": "PMID:1078329", "title": "Bacteriology of the socket in patients with prostheses.", "content": "Fifty-two unilaterally enucleated patients were studied for symptoms, signs, and actual presence of bacteria in the socket and fellow eye. The majority (85%) of asymptomatic sockets were found to have a mixed culture of pathogenic organisms. Sockets had a significantly (P = .001) greater frequency of pathogenic organisms than the fellow eye. Cross contamination does not appear frequently but is a definite possibility. We recommend culture and treatment of the asymptomatic socket before surgery on the remaining eye.", "contents": "Bacteriology of the socket in patients with prostheses. Fifty-two unilaterally enucleated patients were studied for symptoms, signs, and actual presence of bacteria in the socket and fellow eye. The majority (85%) of asymptomatic sockets were found to have a mixed culture of pathogenic organisms. Sockets had a significantly (P = .001) greater frequency of pathogenic organisms than the fellow eye. Cross contamination does not appear frequently but is a definite possibility. We recommend culture and treatment of the asymptomatic socket before surgery on the remaining eye."}
{"id": "PMID:1078330", "title": "Results after vitrectomy in diabetes.", "content": "Eighteen eyes in seventeen patients with diabetic retinopathy were operated on using the vitreous infusion-suction cutter. All had severe loss of vision due to retinal detachment or vitreous hemorrhage, or both. Thirteen eyes had a significant improvement in vision. The surgical management and postoperative problems are described. The results suggest that this technique is useful in selected cases of blindness caused by diabetes.", "contents": "Results after vitrectomy in diabetes. Eighteen eyes in seventeen patients with diabetic retinopathy were operated on using the vitreous infusion-suction cutter. All had severe loss of vision due to retinal detachment or vitreous hemorrhage, or both. Thirteen eyes had a significant improvement in vision. The surgical management and postoperative problems are described. The results suggest that this technique is useful in selected cases of blindness caused by diabetes."}
{"id": "PMID:1078331", "title": "Optic disc observations in glaucoma.", "content": "In glaucomatous eyes, contrary to normal eyes, the blind spot size is not correlated with the disc or cup size, and the size of the optic disc, cup and optic rim also varies. The variation in area of the glaucomatous optic disc correlates with variation in area of the optic cup. The rim width and total rim area in glaucomatous eyes are decreased. In glaucoma the upper, lower and nasal rim widths are about the same, whereas the smallest rim width occurs temporally. Thus, clinically cupping in glaucoma eyes need not be vertically oval.", "contents": "Optic disc observations in glaucoma. In glaucomatous eyes, contrary to normal eyes, the blind spot size is not correlated with the disc or cup size, and the size of the optic disc, cup and optic rim also varies. The variation in area of the glaucomatous optic disc correlates with variation in area of the optic cup. The rim width and total rim area in glaucomatous eyes are decreased. In glaucoma the upper, lower and nasal rim widths are about the same, whereas the smallest rim width occurs temporally. Thus, clinically cupping in glaucoma eyes need not be vertically oval."}
{"id": "PMID:1078332", "title": "Malignant melanoma arising in a choroidal magnacellular nevus (melanocytoma).", "content": "A case is presented of a 55-year-old Caucasian male whose right eye was enucleated for a mixed spindle-A and spindle-B malignant melanoma in 1967. The ophthalmoscopic picture, fluorescein angiography, and overlying peculiar orange pigmentation were suggestive of a malignant lesion. Study of serial sections of the entire lesion made possible the histologic reconstruction of the lesion, clinicopathologic correlation of the yellow-orange pigment, and the location of the nevus and mixed spindle-cell melanoma components.", "contents": "Malignant melanoma arising in a choroidal magnacellular nevus (melanocytoma). A case is presented of a 55-year-old Caucasian male whose right eye was enucleated for a mixed spindle-A and spindle-B malignant melanoma in 1967. The ophthalmoscopic picture, fluorescein angiography, and overlying peculiar orange pigmentation were suggestive of a malignant lesion. Study of serial sections of the entire lesion made possible the histologic reconstruction of the lesion, clinicopathologic correlation of the yellow-orange pigment, and the location of the nevus and mixed spindle-cell melanoma components."}
{"id": "PMID:1078333", "title": "Tumor-associated antibodies in the serum of patients with uveal melanoma.", "content": "Antibodies reacting with autologous and allogeneic cytoplasmic antigens and with autologous surface membrane antigens of uveal melanoma cells were demonstrated in the serum of three patients with relatively small, histologically-proven malignant melanomas of the choroid. The significance of these findings and planned applications of these investigations with regard to diagnosis, prognosis, and therapy of patients with suspected or proven ocular melanomas are discussed.", "contents": "Tumor-associated antibodies in the serum of patients with uveal melanoma. Antibodies reacting with autologous and allogeneic cytoplasmic antigens and with autologous surface membrane antigens of uveal melanoma cells were demonstrated in the serum of three patients with relatively small, histologically-proven malignant melanomas of the choroid. The significance of these findings and planned applications of these investigations with regard to diagnosis, prognosis, and therapy of patients with suspected or proven ocular melanomas are discussed."}
{"id": "PMID:1078334", "title": "Clinical electroretinography by the skin electrode and signal averaged method.", "content": "Conventional electroretinography requires the application of a ring or contact electrode to the anaesthetized cornea. A simplified method for routine clinical electroretinography is described. Skin electrodes are used, signals are appropriately filtered, amplified, passed to a signal averager and displayed on an oscilloscope. Sixteen sweeps are employed and a wave form similar in appearance and frequency content to that obtained by the corneal electrode method, is obtained. Values for the light and dark adapted eye in normal subjects (pupils undilated), are provided and the findings in the stages of retinitis pigmentosa and in other disease states, are noted.", "contents": "Clinical electroretinography by the skin electrode and signal averaged method. Conventional electroretinography requires the application of a ring or contact electrode to the anaesthetized cornea. A simplified method for routine clinical electroretinography is described. Skin electrodes are used, signals are appropriately filtered, amplified, passed to a signal averager and displayed on an oscilloscope. Sixteen sweeps are employed and a wave form similar in appearance and frequency content to that obtained by the corneal electrode method, is obtained. Values for the light and dark adapted eye in normal subjects (pupils undilated), are provided and the findings in the stages of retinitis pigmentosa and in other disease states, are noted."}
{"id": "PMID:1078335", "title": "Adaptation of Jena 5000 photocoagulator to continuous wave laser power source.", "content": "We describe the modifications made in adapting a Jena 5,000 photocoagulator to a tunable dye laser power source and discuss the principles of the tunable dye laser.", "contents": "Adaptation of Jena 5000 photocoagulator to continuous wave laser power source. We describe the modifications made in adapting a Jena 5,000 photocoagulator to a tunable dye laser power source and discuss the principles of the tunable dye laser."}
{"id": "PMID:1078336", "title": "Primary localized conjunctival amyloidosis following strabismus surgery.", "content": "A 13-year-old white boy developed bilateral polypoid conjunctival masses overlying the lateral rectus muscles six months after surgery for divergent strabismus. Histologic examination confirmed the presence of amyloid in these lesions.", "contents": "Primary localized conjunctival amyloidosis following strabismus surgery. A 13-year-old white boy developed bilateral polypoid conjunctival masses overlying the lateral rectus muscles six months after surgery for divergent strabismus. Histologic examination confirmed the presence of amyloid in these lesions."}
{"id": "PMID:1078337", "title": "Strabismus fixus divergens and associated craniofacial anomalies.", "content": "A case of congenital strabismus fixus divergens is described. Other anomalies were vertically oval corneas, high mixed astigmatism, amblyopia, horizontal nystagmus, microcephaly, maxillary hypoplasia, micrognathia, low set large ears, and hyperostosis frontalis interna.", "contents": "Strabismus fixus divergens and associated craniofacial anomalies. A case of congenital strabismus fixus divergens is described. Other anomalies were vertically oval corneas, high mixed astigmatism, amblyopia, horizontal nystagmus, microcephaly, maxillary hypoplasia, micrognathia, low set large ears, and hyperostosis frontalis interna."}
{"id": "PMID:1078338", "title": "The 21 point vision analysis without a phoropter.", "content": "This paper offers a sequence of valid tests, objective in nature, to measure the vision performance and provide an appropriate prescription for an individual who is unable to undergo the demands of a 21 point analytical refraction, through a phoropter, due to age, intelligence or communication difficulty.", "contents": "The 21 point vision analysis without a phoropter. This paper offers a sequence of valid tests, objective in nature, to measure the vision performance and provide an appropriate prescription for an individual who is unable to undergo the demands of a 21 point analytical refraction, through a phoropter, due to age, intelligence or communication difficulty."}
{"id": "PMID:1078339", "title": "Analyzing infants vision: evaluating progress.", "content": "This paper is a further report outlining and analyzing the data from the first 271 infants and toddlers seen at the Infants' Vision Clinic of the Optometric Center of New York during its first two years of operation. The data investigated this paper concerns the evaluation of the progress made by those children who returned to the clinic for continuing visual care.", "contents": "Analyzing infants vision: evaluating progress. This paper is a further report outlining and analyzing the data from the first 271 infants and toddlers seen at the Infants' Vision Clinic of the Optometric Center of New York during its first two years of operation. The data investigated this paper concerns the evaluation of the progress made by those children who returned to the clinic for continuing visual care."}
{"id": "PMID:1078340", "title": "Clinical application of bell retinoscopy.", "content": "The procedure of bell retinoscopy is described in detail, including changes in motion, color, and brightness of reflex. The responses are divided into eight categories. Suggestions are included to guide and direct the optometrist in prescribing lenses on the basis of bell retinoscopy.", "contents": "Clinical application of bell retinoscopy. The procedure of bell retinoscopy is described in detail, including changes in motion, color, and brightness of reflex. The responses are divided into eight categories. Suggestions are included to guide and direct the optometrist in prescribing lenses on the basis of bell retinoscopy."}
{"id": "PMID:1078341", "title": "Children's vision--a 20/20 outlook.", "content": "Twenty years ago, A. M. Skeffington, O.D., talked about the \"uniqueness\" of optometry which included prevention, remediation and enhancement. Fifteen years ago, Arnold Gesell, M.D., urged optometry to pay attention to the preschool years and to examine and supervise (with optometric expertise) the vision of these children and to learn to identify those children who would present difficulties in learning. At the same time, Darell Boyd Harmon, Ph.D., suggested that optometry \"gear up\" for an expected avalanche of visually-related learning problems. Optometry did listen and learn, the avalanche is here and developmental optometry has prospered. This paper briefly explores some of the long and short-range benefits of vision therapy.", "contents": "Children's vision--a 20/20 outlook. Twenty years ago, A. M. Skeffington, O.D., talked about the \"uniqueness\" of optometry which included prevention, remediation and enhancement. Fifteen years ago, Arnold Gesell, M.D., urged optometry to pay attention to the preschool years and to examine and supervise (with optometric expertise) the vision of these children and to learn to identify those children who would present difficulties in learning. At the same time, Darell Boyd Harmon, Ph.D., suggested that optometry \"gear up\" for an expected avalanche of visually-related learning problems. Optometry did listen and learn, the avalanche is here and developmental optometry has prospered. This paper briefly explores some of the long and short-range benefits of vision therapy."}
{"id": "PMID:1078343", "title": "A multidisciplinary evaluation of the child with a visually related learning disability.", "content": "It is conceded that not all learning disabilities result from vision or visual perceptual problems. Optometrists are advised to familiarize themselves with techniques employed by other disciplines dealing with learning disabled children. It is suggested that they direct themselves to the myriad of variables involved in the care of the under achieving or non-achieving child so that their own specific techniques will become of greater value to the patient.", "contents": "A multidisciplinary evaluation of the child with a visually related learning disability. It is conceded that not all learning disabilities result from vision or visual perceptual problems. Optometrists are advised to familiarize themselves with techniques employed by other disciplines dealing with learning disabled children. It is suggested that they direct themselves to the myriad of variables involved in the care of the under achieving or non-achieving child so that their own specific techniques will become of greater value to the patient."}
{"id": "PMID:1078344", "title": "Optometric intraprofessional referral of visual dysfunctions.", "content": "Every optometrist has the professional responsibility to detect visual dysfunctions. If optometrists do not wish to provide vision therapy in their offices they have an obligation to refer. Simple screening tests and guidelines for referral are described.", "contents": "Optometric intraprofessional referral of visual dysfunctions. Every optometrist has the professional responsibility to detect visual dysfunctions. If optometrists do not wish to provide vision therapy in their offices they have an obligation to refer. Simple screening tests and guidelines for referral are described."}
{"id": "PMID:1078345", "title": "A training program for elementary school teachers. Optometrist-teacher communications.", "content": "Communication between optometrists and teachers is critical to optometric involvement in interdisciplinary team approaches to learning disabilities. The classroom teacher may be a prime source of referrals to the general optometrist as well as the vision development specialist. Optometry's contribution to classroom learning cannot be fully realized without sound optometrist-teacher communication. The basic design of a training program to enhance optometrist-teacher communications is presented in this paper. The individual optometrist may modify and supplement the program to suit the special needs of the teachers involved.", "contents": "A training program for elementary school teachers. Optometrist-teacher communications. Communication between optometrists and teachers is critical to optometric involvement in interdisciplinary team approaches to learning disabilities. The classroom teacher may be a prime source of referrals to the general optometrist as well as the vision development specialist. Optometry's contribution to classroom learning cannot be fully realized without sound optometrist-teacher communication. The basic design of a training program to enhance optometrist-teacher communications is presented in this paper. The individual optometrist may modify and supplement the program to suit the special needs of the teachers involved."}
{"id": "PMID:1078347", "title": "Optometric care for a child manifesting qualities of autism.", "content": "S.G. still manifests many of the behavior characteristics of an autistic child. Optometric vision care was significant in that it did allow S.G. to develop better visual contact, longer periods of meaningful attentiveness, and to establish some rudimentary relationships with situations and people. His visual contact is still quite limited to things and space at relatively close distances. Binocular functioning has markedly improved.", "contents": "Optometric care for a child manifesting qualities of autism. S.G. still manifests many of the behavior characteristics of an autistic child. Optometric vision care was significant in that it did allow S.G. to develop better visual contact, longer periods of meaningful attentiveness, and to establish some rudimentary relationships with situations and people. His visual contact is still quite limited to things and space at relatively close distances. Binocular functioning has markedly improved."}
{"id": "PMID:1078351", "title": "Optometric intervention in the control of juvenile delinquents.", "content": "We are losing the battle against crime and delinquency and we have to continue to lose so long as we keep developing juvenile delinquents. In order to prevent crime, we have to prevent juvenile delinquency for criminals are not born, they develop from juvenile delinquents. Research studies in Chattanooga, Tennessee, show that the youngsters brought before the juvenile court have many different kinds of problems, but one problem that almost all have, other than the drug scene kid, is that they cannot read adequately. Many high school students are reading at first, second and third grade level; 91 percent to 94 percent of our juvenile delinquents are having reading and learning difficulties in the classroom. Consequently, they are all socially promoted and are facing the sting of failure which leads to truancy and conflict with school authorities. These children go from frustration to frustration and become behavior problems in the classroom. The teachers of the first, second, and third grades know which children are having difficulty with reading and will have possible behavioral problems, possibly becoming juvenile delinquents. This writer believes there are five essential preconditions which contribute to one's becoming a juvenile delinquent. Robert had them all: 1. Lack of proper supervision by the parents is the first precondition which contributes to juvenile delinquency. The family is the first and basic institution for the development of the child's emotional intellectual, moral, spiritual, physical and social behavior. 2. Improper discipline is the second factor. Experience teaches us that permissiveness should be controlled at an early age, and too much discipline is just as bad as too little discipline.(ABSTRACT TRUNCATED AT 250 WORDS)", "contents": "Optometric intervention in the control of juvenile delinquents. We are losing the battle against crime and delinquency and we have to continue to lose so long as we keep developing juvenile delinquents. In order to prevent crime, we have to prevent juvenile delinquency for criminals are not born, they develop from juvenile delinquents. Research studies in Chattanooga, Tennessee, show that the youngsters brought before the juvenile court have many different kinds of problems, but one problem that almost all have, other than the drug scene kid, is that they cannot read adequately. Many high school students are reading at first, second and third grade level; 91 percent to 94 percent of our juvenile delinquents are having reading and learning difficulties in the classroom. Consequently, they are all socially promoted and are facing the sting of failure which leads to truancy and conflict with school authorities. These children go from frustration to frustration and become behavior problems in the classroom. The teachers of the first, second, and third grades know which children are having difficulty with reading and will have possible behavioral problems, possibly becoming juvenile delinquents. This writer believes there are five essential preconditions which contribute to one's becoming a juvenile delinquent. Robert had them all: 1. Lack of proper supervision by the parents is the first precondition which contributes to juvenile delinquency. The family is the first and basic institution for the development of the child's emotional intellectual, moral, spiritual, physical and social behavior. 2. Improper discipline is the second factor. Experience teaches us that permissiveness should be controlled at an early age, and too much discipline is just as bad as too little discipline.(ABSTRACT TRUNCATED AT 250 WORDS)"}
{"id": "PMID:1078355", "title": "Optometric manpower resources--1973. Part II.", "content": "The Optometric Manpower Resources Project, funded under government contract since June of 1971, has completed its 1973 national census of licensed optometric manpower in the United States. The first article in this series of three reported on the distribution of optometrists by census region and the current ratio of practicing optometrists to civilian population. This second article reports on the principal forms of employment of active optometrists, the distribution of number of years in practice and practice size. The data indicate that there has been a shift away from self-employment (88% in 1968, 77% in 1973) into more varied forms of salaried employment, especially among those in practice fewer than ten years. Practice size is shown to peak at between fifteen and twenty-four years in practice with a median of approximately 5,400 patients.", "contents": "Optometric manpower resources--1973. Part II. The Optometric Manpower Resources Project, funded under government contract since June of 1971, has completed its 1973 national census of licensed optometric manpower in the United States. The first article in this series of three reported on the distribution of optometrists by census region and the current ratio of practicing optometrists to civilian population. This second article reports on the principal forms of employment of active optometrists, the distribution of number of years in practice and practice size. The data indicate that there has been a shift away from self-employment (88% in 1968, 77% in 1973) into more varied forms of salaried employment, especially among those in practice fewer than ten years. Practice size is shown to peak at between fifteen and twenty-four years in practice with a median of approximately 5,400 patients."}
{"id": "PMID:1078356", "title": "Rotational characteristics of the Warner-Lambert Softcon hydrophilic contact lenses.", "content": "The rotational characteristics of seven Warner-Lambert Softcon soft hydrophilic contact lenses were monitored on the subject's eye by direct observation and photographically for approximately 30 minutes. Lens parameters which altered the rate and direction of rotation were recorded and evaluated.", "contents": "Rotational characteristics of the Warner-Lambert Softcon hydrophilic contact lenses. The rotational characteristics of seven Warner-Lambert Softcon soft hydrophilic contact lenses were monitored on the subject's eye by direct observation and photographically for approximately 30 minutes. Lens parameters which altered the rate and direction of rotation were recorded and evaluated."}
{"id": "PMID:1078357", "title": "Mydriatic effects using low concentrations of phenylephrine hydrochloride.", "content": "The dose response relationship of different concentrations of phenylephrine with and without prior application of topical anesthetic was measured to determine the minimum concentration which produced maximum mydriasis. As little as 0.125% phenylephrine caused mydriasis in some subjects and maximum mydriasis was achieved with 2%. Concentrations greater than 2% produced very little additional mydriasis. A therapeutically effective dose could be as low as 1% with prior installation of a topical anesthetic.", "contents": "Mydriatic effects using low concentrations of phenylephrine hydrochloride. The dose response relationship of different concentrations of phenylephrine with and without prior application of topical anesthetic was measured to determine the minimum concentration which produced maximum mydriasis. As little as 0.125% phenylephrine caused mydriasis in some subjects and maximum mydriasis was achieved with 2%. Concentrations greater than 2% produced very little additional mydriasis. A therapeutically effective dose could be as low as 1% with prior installation of a topical anesthetic."}
{"id": "PMID:1078358", "title": "Waxed windshields are hazardous in the rain.", "content": "Seven automobiles were washed and waxed at four car washes. Photographic determinations were made of the glare produced by the wet or dry waxed windshield in a headlight beam. When wet, the waxed windshields scattered three times more light than in the normal human eye. The wet wax scattering was 24.8 times higher than when dry. No wax residues should be permitted on windshields and the National Highway Traffic Safety Administration should issue a mandatory windshield cleaning requirement after waxing.", "contents": "Waxed windshields are hazardous in the rain. Seven automobiles were washed and waxed at four car washes. Photographic determinations were made of the glare produced by the wet or dry waxed windshield in a headlight beam. When wet, the waxed windshields scattered three times more light than in the normal human eye. The wet wax scattering was 24.8 times higher than when dry. No wax residues should be permitted on windshields and the National Highway Traffic Safety Administration should issue a mandatory windshield cleaning requirement after waxing."}
{"id": "PMID:1078359", "title": "A vision care module within a prototype HMO.", "content": "The Health Maintenance Organization (HMO) concept is dedicated to the prevention of disease and disability. Organizations incorporating this concept must include vision care if complete service is to be offered. Described in this paper is one such HMO, Group Health Cooperative of Puget Sound, with details of the successful integration of optometric vision care into this medically oriented, multidisciplinary setting.", "contents": "A vision care module within a prototype HMO. The Health Maintenance Organization (HMO) concept is dedicated to the prevention of disease and disability. Organizations incorporating this concept must include vision care if complete service is to be offered. Described in this paper is one such HMO, Group Health Cooperative of Puget Sound, with details of the successful integration of optometric vision care into this medically oriented, multidisciplinary setting."}
{"id": "PMID:1078360", "title": "Paraprofessional utilization in optometry.", "content": "Issues that surround the use of paraprofessionals in optometry are examined. The experiences and findings from paraprofessional programs in other health disciplines are reviewed as they relate to optometry. Paraprofessional utilization is a significant yet neglected alternative that has the potential of relieving the increasing manpower shortage in optometry. This concept is reviewed in terms of its effects on productivity and quality of care. The viability of this concept has been found to be dependent upon the subjective acceptance of paraprofessionals by the health professional as well as the consumer. Patient attitudes and acceptance are largely determined by the professional's acceptance. Values and beliefs held by the optometrist or some other discipline may manifest themselves as significant barriers to increased utilization of paraoptometric personnel. The socialization process must begin during the years of professional education and early years of clinical experience.", "contents": "Paraprofessional utilization in optometry. Issues that surround the use of paraprofessionals in optometry are examined. The experiences and findings from paraprofessional programs in other health disciplines are reviewed as they relate to optometry. Paraprofessional utilization is a significant yet neglected alternative that has the potential of relieving the increasing manpower shortage in optometry. This concept is reviewed in terms of its effects on productivity and quality of care. The viability of this concept has been found to be dependent upon the subjective acceptance of paraprofessionals by the health professional as well as the consumer. Patient attitudes and acceptance are largely determined by the professional's acceptance. Values and beliefs held by the optometrist or some other discipline may manifest themselves as significant barriers to increased utilization of paraoptometric personnel. The socialization process must begin during the years of professional education and early years of clinical experience."}
{"id": "PMID:1078361", "title": "Osmotic edema associated with contact lens adaptation.", "content": "When associated with the new contact lens patient, corneal edema may stem from quite different physiological bases, e.g. relative anoxia or osmotic imbalance. A quantitative overview of the osmotic origins of epithelial edema is given here.", "contents": "Osmotic edema associated with contact lens adaptation. When associated with the new contact lens patient, corneal edema may stem from quite different physiological bases, e.g. relative anoxia or osmotic imbalance. A quantitative overview of the osmotic origins of epithelial edema is given here."}
{"id": "PMID:1078364", "title": "Optometry and the underprivileged--an investigative report.", "content": "In this essay I have taken the topic, \"Optometry and the Underprivileged,\" and approached it as an investigative report. This investigation found me talking with people in all walks of life and visiting many institutions and health care facilities. Some of the people discussed in the report and their conditions represent composites of the real situations I encountered. Their names are fictitious and to the best of my knowledge these people do not exist. The report is intended to be informative, constructively critical, unboring, and positive. If I have accomplished this, then my purpose has been fulfilled.", "contents": "Optometry and the underprivileged--an investigative report. In this essay I have taken the topic, \"Optometry and the Underprivileged,\" and approached it as an investigative report. This investigation found me talking with people in all walks of life and visiting many institutions and health care facilities. Some of the people discussed in the report and their conditions represent composites of the real situations I encountered. Their names are fictitious and to the best of my knowledge these people do not exist. The report is intended to be informative, constructively critical, unboring, and positive. If I have accomplished this, then my purpose has been fulfilled."}
{"id": "PMID:1078365", "title": "Optometric manpower resources--1973. Part III.", "content": "The Optometric Manpower Resources Project, funded under government contract since June of 1971, has completed its 1973 national census of licensed optometric manpower in the United States. The first article in this series of three reported on the distribution of optometrists by census region and the current ratios of practicing optometrists to civilian population. The second article reported on the principal forms of employment of active optometrists, the number of years in practice and practice size. This third article reports on the age and sex distributions of licensed optometrists, their racial/ethnic composition, their pattern of specialization, and the schools/colleges of optometry from which they graduated. The data indicate that almost 50% of active optometrists were over 50 years of age. Approximately 90% of male optometrists and only 58.5% of female optometrists were active. The data also indicate that one-third of all optometrists active in 1973 graduated from four schools/colleges of optometry.", "contents": "Optometric manpower resources--1973. Part III. The Optometric Manpower Resources Project, funded under government contract since June of 1971, has completed its 1973 national census of licensed optometric manpower in the United States. The first article in this series of three reported on the distribution of optometrists by census region and the current ratios of practicing optometrists to civilian population. The second article reported on the principal forms of employment of active optometrists, the number of years in practice and practice size. This third article reports on the age and sex distributions of licensed optometrists, their racial/ethnic composition, their pattern of specialization, and the schools/colleges of optometry from which they graduated. The data indicate that almost 50% of active optometrists were over 50 years of age. Approximately 90% of male optometrists and only 58.5% of female optometrists were active. The data also indicate that one-third of all optometrists active in 1973 graduated from four schools/colleges of optometry."}
{"id": "PMID:1078366", "title": "The role of the technician in optometric practice.", "content": "Members of the Indiana Optometric Association were asked to rate each of the duties which Indiana University optometric technician students are currently taught to perform. Rated on a four point scale from most desirable to undesirable, the duties deemed most important by respondents were those having to do with the dispensing of glasses and contact lenses as well as the usual receptionist and secretarial duties. The duties receiving the lowest ratings were keratometry and tonometry. However, there is good general agreement between the school and the profession concerning the content of the optometric technician program.", "contents": "The role of the technician in optometric practice. Members of the Indiana Optometric Association were asked to rate each of the duties which Indiana University optometric technician students are currently taught to perform. Rated on a four point scale from most desirable to undesirable, the duties deemed most important by respondents were those having to do with the dispensing of glasses and contact lenses as well as the usual receptionist and secretarial duties. The duties receiving the lowest ratings were keratometry and tonometry. However, there is good general agreement between the school and the profession concerning the content of the optometric technician program."}
{"id": "PMID:1078367", "title": "Leadership and interpersonal need compatibilities between optometrists and optometric assistants.", "content": "A comparison of optometrists' leadership style and the preferred leadership style of their optometric assistants as well as both groups' interpersonal need orientations indicated: 1) optometrists' consideration and initiating structure orientations were lower than those preferred by their assistants, structure was significantly lower (p less than .01) while consideration approached significance (p = .08); 2) optometrists had significantly lower needs for expressing control than their assistants had for wanting to be controlled (p less than .01), assistants had significantly higher needs for expressing participation and inclusion than optometrists had for wanting to be included (p less than .05), and optometrists had significantly higher needs for wanting to be controlled and influenced than their assistants had to control and influence them (p less than .05). Implications for the optometrist-assistant working relationship are discussed.", "contents": "Leadership and interpersonal need compatibilities between optometrists and optometric assistants. A comparison of optometrists' leadership style and the preferred leadership style of their optometric assistants as well as both groups' interpersonal need orientations indicated: 1) optometrists' consideration and initiating structure orientations were lower than those preferred by their assistants, structure was significantly lower (p less than .01) while consideration approached significance (p = .08); 2) optometrists had significantly lower needs for expressing control than their assistants had for wanting to be controlled (p less than .01), assistants had significantly higher needs for expressing participation and inclusion than optometrists had for wanting to be included (p less than .05), and optometrists had significantly higher needs for wanting to be controlled and influenced than their assistants had to control and influence them (p less than .05). Implications for the optometrist-assistant working relationship are discussed."}
{"id": "PMID:1078368", "title": "An experiment in health careers recruitment: a summer program at Indiana University.", "content": "During the summer of 1973, the Indiana University Division of Optometry, via a Special Project Grant from the National Institutes of Health, conducted its first Summer Institute in the Health Related Professions for High School and College Students. Twenty-seven high school students and twenty-nine college students were brought to Indiana University for intensive sessions in career awareness, curriculum and financial counseling, and academic coursework. Assisted by the staffs of Indiana Health Careers, Inc. and the Indiana University Coordinators for School Science, the Institute awakened new interests and goals, relative to the health career prospectus, within the trainees. As a direct consequence of the summer program, four participants in the college institute were matriculated into schools of medicine, pharmacy, and optometry during the semester following the culmination of the Institute; ten more are participating in the followup program for continued guidance and counseling, seeking 1974 entrance into health professions schools and colleges. This experiment in health professions recruitment for minority, disadvantaged, and low-income students proved to be a viable method of increasing the manpower pool of health professionals.", "contents": "An experiment in health careers recruitment: a summer program at Indiana University. During the summer of 1973, the Indiana University Division of Optometry, via a Special Project Grant from the National Institutes of Health, conducted its first Summer Institute in the Health Related Professions for High School and College Students. Twenty-seven high school students and twenty-nine college students were brought to Indiana University for intensive sessions in career awareness, curriculum and financial counseling, and academic coursework. Assisted by the staffs of Indiana Health Careers, Inc. and the Indiana University Coordinators for School Science, the Institute awakened new interests and goals, relative to the health career prospectus, within the trainees. As a direct consequence of the summer program, four participants in the college institute were matriculated into schools of medicine, pharmacy, and optometry during the semester following the culmination of the Institute; ten more are participating in the followup program for continued guidance and counseling, seeking 1974 entrance into health professions schools and colleges. This experiment in health professions recruitment for minority, disadvantaged, and low-income students proved to be a viable method of increasing the manpower pool of health professionals."}
{"id": "PMID:1078379", "title": "Health law in 1985--implications for the optometric profession.", "content": "In conclusion, I believe that you should seek to expand the scope of optometry into areas now regarded as exclusively medical practice, and devote less time and effort to protecting your rear from the encroachment of the opticians. I suggest this because it follows a seemingly natural process of expanding upward that one finds in other professions and occupations in the health field. Second, I have suggested that, to achieve an expanded scope of optometry, there may have to be some sacrifice of the concept of the independent, entrepreneurial practitioner in optometry. Even the medical profession is recognizing that the individual entrepreneurial physician is becoming less of a factor than he was before, and the future is not particularly bright for him further down the road. Third, to the extent you can influence the forum where the struggle over the expanded practice of optometry takes place, you may increase the likelihood that the expanded role will be recognized by law sooner. I have already alluded to the idea that costs of health services and funding mechanisms are intimately tied to the law related to optometric practice in the future. As you know from the legislative struggles in many states over inclusion of optometric services within state Medicaid programs, and freedom of choice legislation that required an insurer to pay any legally recognized practitioner who could provide the service, rather than physicians exclusively, the optometric profession is well aware how closely the success of a profession is linked to access to third party payment.(ABSTRACT TRUNCATED AT 250 WORDS)", "contents": "Health law in 1985--implications for the optometric profession. In conclusion, I believe that you should seek to expand the scope of optometry into areas now regarded as exclusively medical practice, and devote less time and effort to protecting your rear from the encroachment of the opticians. I suggest this because it follows a seemingly natural process of expanding upward that one finds in other professions and occupations in the health field. Second, I have suggested that, to achieve an expanded scope of optometry, there may have to be some sacrifice of the concept of the independent, entrepreneurial practitioner in optometry. Even the medical profession is recognizing that the individual entrepreneurial physician is becoming less of a factor than he was before, and the future is not particularly bright for him further down the road. Third, to the extent you can influence the forum where the struggle over the expanded practice of optometry takes place, you may increase the likelihood that the expanded role will be recognized by law sooner. I have already alluded to the idea that costs of health services and funding mechanisms are intimately tied to the law related to optometric practice in the future. As you know from the legislative struggles in many states over inclusion of optometric services within state Medicaid programs, and freedom of choice legislation that required an insurer to pay any legally recognized practitioner who could provide the service, rather than physicians exclusively, the optometric profession is well aware how closely the success of a profession is linked to access to third party payment.(ABSTRACT TRUNCATED AT 250 WORDS)"}
{"id": "PMID:1078385", "title": "Understanding fluorescein contact lens photography: equipment and technique.", "content": "A basic consideration of the utilization of close-up photography in the ophthalmic health care field is discussed. These principles can be applied to fluorescein contact lens photography using a hand-held camera. The discussion is divided into two areas: basic theory and equipment is reviewed, and a description of the clinical technique is described.", "contents": "Understanding fluorescein contact lens photography: equipment and technique. A basic consideration of the utilization of close-up photography in the ophthalmic health care field is discussed. These principles can be applied to fluorescein contact lens photography using a hand-held camera. The discussion is divided into two areas: basic theory and equipment is reviewed, and a description of the clinical technique is described."}
{"id": "PMID:1078386", "title": "Orthokeratology: changes to the corneal curvature and the effect on refractive power due to the sagittal length change.", "content": "Little has been published to date on orthokeratology which can explain what changes the eye actually goes through. Many professionals are waiting for this evidence before judging this controversial technique. With orthokeratology the corneal curves are changed and present a definite pattern. There are a number of changes which must occur when the cornea is flattened which reduce the power of the eye beyond that amount of flattening. This paper examines one of these unknowns.", "contents": "Orthokeratology: changes to the corneal curvature and the effect on refractive power due to the sagittal length change. Little has been published to date on orthokeratology which can explain what changes the eye actually goes through. Many professionals are waiting for this evidence before judging this controversial technique. With orthokeratology the corneal curves are changed and present a definite pattern. There are a number of changes which must occur when the cornea is flattened which reduce the power of the eye beyond that amount of flattening. This paper examines one of these unknowns."}
{"id": "PMID:1078387", "title": "The efficacy of frosted contact lenses in controlling flare.", "content": "Improved techniques are rapidly increasing alternatives to the contact lens practitioner to aid in his efforts to afford maximum comfort and efficacy to the vision of his patients. The purpose of this research study was to determine if subjective symptoms of flare could be controlled, to a significant degree, with a frosted contact lens.", "contents": "The efficacy of frosted contact lenses in controlling flare. Improved techniques are rapidly increasing alternatives to the contact lens practitioner to aid in his efforts to afford maximum comfort and efficacy to the vision of his patients. The purpose of this research study was to determine if subjective symptoms of flare could be controlled, to a significant degree, with a frosted contact lens."}
{"id": "PMID:1078391", "title": "The influence of a high level of corn oil on rat serum lipoproteins.", "content": "Although the stated requirement for linoleic acid in humans is less than 2% of the dietary calories, recently there has been considerable emphasis on the necessity to substitute dietary polyunsaturates for saturates in order to reduce serum cholesterol levels. In this study we have sought to determine the nutritional consequences of feeding a very high level of linoleate to rats. Three groups of thirty adult animals each were fed a semipurified diet consisting by weight of casein 17%; mineral mixture 5.5%; vitamin mixture in glucose 2.2%; cellulose fiber 3.0%; and corn oil 0% (group A), 10% (group B) or 40% (group C), which was provided at the expense of glucose. At the end of four weeks on the diets, blood was obtained in the fasting state from 16 rats in each group. The serum was ultracentrifugally fractionated into six classes of lipoproteins and analyzed for lipid composition and protein content. Disc gel electrophoresis using lipid and protein stains established that the various lipoprotein subclasses were reasonably free of adjacent density fractions. Although the total serum cholesterol levels were practically the same in the three groups, the cholesterol moiety of the major low density lipoproteins, LDL2 (d 1.019-1.050), but not of very low density lipoproteins, VLDL (d 1.006) or low density lipoproteins, LDL1 (d 1.006-1.019), was substantially and very significantly increased in rats fed the high level of corn oil as compared to the other groups. The concentration of the very low density lipoproteins was significantly lower in group C than in the groups A and B. The LDL2 concentration but not that of LDL1 was significantly greater in group C as compared to group A. The cholesterol/total lipid ratio was significantly greater in both LDL2 and LDL1 but not in VLDL of group C as compared with group A. The serum high density lipoproteins were relatively less influenced by the ingestion of an excessive level of corn oil at this time period. The serum lipoprotein levels as well as their lipid composition were generally similar in groups A and B and suggested that a moderate level (5%) of dietary linoleate did not cause any untoward changes in rats. On the basis of current information on the metabolism of lipoproteins, it has been proposed that the increase in rat serum LDL2 of group C reflects the status of the liver and that a large portion of the cholesterol moiety of LDL2 is perhaps derived from the liver while the protein and phospholipid portions may represent remnants of VLDL catabolism.(ABSTRACT TRUNCATED AT 400 WORDS)", "contents": "The influence of a high level of corn oil on rat serum lipoproteins. Although the stated requirement for linoleic acid in humans is less than 2% of the dietary calories, recently there has been considerable emphasis on the necessity to substitute dietary polyunsaturates for saturates in order to reduce serum cholesterol levels. In this study we have sought to determine the nutritional consequences of feeding a very high level of linoleate to rats. Three groups of thirty adult animals each were fed a semipurified diet consisting by weight of casein 17%; mineral mixture 5.5%; vitamin mixture in glucose 2.2%; cellulose fiber 3.0%; and corn oil 0% (group A), 10% (group B) or 40% (group C), which was provided at the expense of glucose. At the end of four weeks on the diets, blood was obtained in the fasting state from 16 rats in each group. The serum was ultracentrifugally fractionated into six classes of lipoproteins and analyzed for lipid composition and protein content. Disc gel electrophoresis using lipid and protein stains established that the various lipoprotein subclasses were reasonably free of adjacent density fractions. Although the total serum cholesterol levels were practically the same in the three groups, the cholesterol moiety of the major low density lipoproteins, LDL2 (d 1.019-1.050), but not of very low density lipoproteins, VLDL (d 1.006) or low density lipoproteins, LDL1 (d 1.006-1.019), was substantially and very significantly increased in rats fed the high level of corn oil as compared to the other groups. The concentration of the very low density lipoproteins was significantly lower in group C than in the groups A and B. The LDL2 concentration but not that of LDL1 was significantly greater in group C as compared to group A. The cholesterol/total lipid ratio was significantly greater in both LDL2 and LDL1 but not in VLDL of group C as compared with group A. The serum high density lipoproteins were relatively less influenced by the ingestion of an excessive level of corn oil at this time period. The serum lipoprotein levels as well as their lipid composition were generally similar in groups A and B and suggested that a moderate level (5%) of dietary linoleate did not cause any untoward changes in rats. On the basis of current information on the metabolism of lipoproteins, it has been proposed that the increase in rat serum LDL2 of group C reflects the status of the liver and that a large portion of the cholesterol moiety of LDL2 is perhaps derived from the liver while the protein and phospholipid portions may represent remnants of VLDL catabolism.(ABSTRACT TRUNCATED AT 400 WORDS)"}
{"id": "PMID:1078392", "title": "Age-related interrelationships of blood pressure and arterial sterol accumulation in spontaneously atherosclerosis-susceptible and atherosclerosis-resistant pigeons.", "content": "Interrelationships of arterial sterol accumulation and blood pressures were examined in age-matched White Carneau and Show Racer pigeons. It was found that the systolic blood pressure of White Carneaux changed from 155.0 mm Hg at nine months of age to 168.8 mm Hg at 17 months of age (P less than 0.005) while that of the Show Racers did not show any age-related increases. In White Carneaux, the total sterol content in the aorta increased by 43.6% from 9 months to 17 months of age (P less than 0.001) with major changes in the steryl ester fraction while no such changes were evident in the Show Racers. This indicates interrelationships of the arterial sterol content and blood pressure in the White Carneau pigeon.", "contents": "Age-related interrelationships of blood pressure and arterial sterol accumulation in spontaneously atherosclerosis-susceptible and atherosclerosis-resistant pigeons. Interrelationships of arterial sterol accumulation and blood pressures were examined in age-matched White Carneau and Show Racer pigeons. It was found that the systolic blood pressure of White Carneaux changed from 155.0 mm Hg at nine months of age to 168.8 mm Hg at 17 months of age (P less than 0.005) while that of the Show Racers did not show any age-related increases. In White Carneaux, the total sterol content in the aorta increased by 43.6% from 9 months to 17 months of age (P less than 0.001) with major changes in the steryl ester fraction while no such changes were evident in the Show Racers. This indicates interrelationships of the arterial sterol content and blood pressure in the White Carneau pigeon."}
{"id": "PMID:1078393", "title": "ABO blood groups and age groups in surgical venous thromboembolism.", "content": "In a series of cases of surgical thromboembolism there were significant age differences in the ratio of cases of blood group A to those of blood group O, the ratio being highest in the youngest age groups.", "contents": "ABO blood groups and age groups in surgical venous thromboembolism. In a series of cases of surgical thromboembolism there were significant age differences in the ratio of cases of blood group A to those of blood group O, the ratio being highest in the youngest age groups."}
{"id": "PMID:1078394", "title": "Familial and acquired type V hyperlipoproteinemia.", "content": "The nature of Type V hyperlipoproteinemia including mode of presentation, prominent clinical and biochemical features, and genetics, was examined in 29 adults presenting with the Type V lipoprotein phenotype. Initially 23 of the 29 patients had various metabolic stimuli (diabetes out of control, estrogenic agents, pancreatitis, ethanolism) superposed on their acute hypertriglyceridemia. After metabolic stabilization, 17 of the 29 subjects were shown to have familial hypertriglyceridemia. In the 17 kindreds with familial hypertriglyceridemia, the lack of a specific, distinctive genetic marker for the Type V genotype and for the Type IV genotype restricts the conclusion that the pattern of inheritance was consistent with an autosomal dominant trait.", "contents": "Familial and acquired type V hyperlipoproteinemia. The nature of Type V hyperlipoproteinemia including mode of presentation, prominent clinical and biochemical features, and genetics, was examined in 29 adults presenting with the Type V lipoprotein phenotype. Initially 23 of the 29 patients had various metabolic stimuli (diabetes out of control, estrogenic agents, pancreatitis, ethanolism) superposed on their acute hypertriglyceridemia. After metabolic stabilization, 17 of the 29 subjects were shown to have familial hypertriglyceridemia. In the 17 kindreds with familial hypertriglyceridemia, the lack of a specific, distinctive genetic marker for the Type V genotype and for the Type IV genotype restricts the conclusion that the pattern of inheritance was consistent with an autosomal dominant trait."}
{"id": "PMID:1078398", "title": "An analysis of arcuate staining with the B&L SOFLENS. Part I.", "content": "A survey of 100 of our patients wearing Bausch & Lomb hydrophilic lenses shows that 38.5 per cent demonstrate corneal staining. The type of corneal staining that we are concerned with is epithelial, superficial, arcuate and usually located peripherally in an area between four and eight o'clock.", "contents": "An analysis of arcuate staining with the B&L SOFLENS. Part I. A survey of 100 of our patients wearing Bausch & Lomb hydrophilic lenses shows that 38.5 per cent demonstrate corneal staining. The type of corneal staining that we are concerned with is epithelial, superficial, arcuate and usually located peripherally in an area between four and eight o'clock."}
{"id": "PMID:1078395", "title": "780 SE: a new type of hypolipemic agent. Comparative assays in rats.", "content": "The effect of 1-(m-trifluoromethylphenyl)-2-(beta-benzoyloxyethyl)-amino- propane hydrochloride (780 SE) on serum lipids, blood glucose and liver weight was studied in 4 experimental models, and compared with that of clofibrate and tiadenol. When rats were given a daily oral dose of 25 mg/kg or 50 mg/kg of 780 SE for 5 days a marked reduction of serum triglycerides and liver weight was observed. The decreases were more pronounced than those in rats treated with 50 mg/kg or 100 mg/kg of clofibrate or tiadenol. On the other hand, a reduction of serum cholesterol was only observed in the groups given clofibrate and tiadenol. These differences could be explained on the basis of the mechanism of action of the different drugs. Only 780 SE induced a decrease in blood sugar level, a reduction of plasma insulin concentration and restored the insulin sensitivity to a normal value in obese animals. There was a significant decrease in liver weight of 780 SE treated rats, whereas clofibrate and tiadenol cause hepatomegaly.", "contents": "780 SE: a new type of hypolipemic agent. Comparative assays in rats. The effect of 1-(m-trifluoromethylphenyl)-2-(beta-benzoyloxyethyl)-amino- propane hydrochloride (780 SE) on serum lipids, blood glucose and liver weight was studied in 4 experimental models, and compared with that of clofibrate and tiadenol. When rats were given a daily oral dose of 25 mg/kg or 50 mg/kg of 780 SE for 5 days a marked reduction of serum triglycerides and liver weight was observed. The decreases were more pronounced than those in rats treated with 50 mg/kg or 100 mg/kg of clofibrate or tiadenol. On the other hand, a reduction of serum cholesterol was only observed in the groups given clofibrate and tiadenol. These differences could be explained on the basis of the mechanism of action of the different drugs. Only 780 SE induced a decrease in blood sugar level, a reduction of plasma insulin concentration and restored the insulin sensitivity to a normal value in obese animals. There was a significant decrease in liver weight of 780 SE treated rats, whereas clofibrate and tiadenol cause hepatomegaly."}
{"id": "PMID:1078399", "title": "Variation in refractive error and corneal curvature after wearing hydrophilic contact lenses.", "content": "Twenty-eight eyes of people who have successfully worn hydrophilic contact lenses were monitored during a period after removal of their contact lenses. The results are compared to similar studies of wearers of non-flexible contact lens. While both curvature and refractive changes in a number of eyes stabilized at about one week, others did not stabilize until three weeks or more.", "contents": "Variation in refractive error and corneal curvature after wearing hydrophilic contact lenses. Twenty-eight eyes of people who have successfully worn hydrophilic contact lenses were monitored during a period after removal of their contact lenses. The results are compared to similar studies of wearers of non-flexible contact lens. While both curvature and refractive changes in a number of eyes stabilized at about one week, others did not stabilize until three weeks or more."}
{"id": "PMID:1078396", "title": "Distribution of cholesterol and triglycerides among lipoprotein fractions in fat-fed rabbits at different levels of serum cholesterol.", "content": "The serum lipoproteins of rabbits given semisynthetic cholesterol-free diets containing coconut oil or butter or a conventional rabbit chow supplemented with cholesterol, were studied by preparative ultracentrifugation and electrophoresis. (1) All three diets elevated the total cholesterol level but only the coconut oil diet markedly increased the triglyceride (TG) content in addition. All ultracentrifugation fractions showed elevated cholesterol/TG ratios, and this was especially evident for the cholesterol diet. In the hyperlipidemic rabbits cholesterol was therefore mainly transported in lipoproteins with a changed lipid composition. (2) The lipid levels of the \"HDL\" fraction were more or less unaffected by the lipid concentration in whole serum. In the total serum cholesterol ranges 150-500 (coconut oil diet) and 100-300 mg/100 ml (cholesterol diet), most cholesterol was transported as \"LDL\" cholesterol. This latter fraction reached maximum cholesterol concentrations of about 350 (coconut oil diet) and 400 mg/100 ml (cholesterol diet) at total cholesterol levels of approximately 600 and 1200 mg/100 ml serum, respectively. At still higher levels of total cholesterol, the whole increment was concentrated to the \"VLDL\" fraction. (3) With semisynthetic diets in the whole cholesterol range 250-400 mg/100 ml it was possible, with respect to cholesterol, to induce fairly similar concentrations and distributions to those seen in man, with about 60% transported as \"LDL\", 30% as \"VLDL\" and 10% as \"HDL\" cholesterol with the coconut oil diet and 65%, 20% and 15%, respectively, with the butter diet. Such experimental conditions seem to be suitable for testing the hypocholesterolemic action of drugs intended for human hyperlipidemia Type II. (4) Compared with earlier investigations on rabbits, the present distribution study suggests that the degree of aortic lipid infiltration in cholesterol-fed rabbits is better related to the levels of \"LDL\" than to \"VLDL\" cholesterol.", "contents": "Distribution of cholesterol and triglycerides among lipoprotein fractions in fat-fed rabbits at different levels of serum cholesterol. The serum lipoproteins of rabbits given semisynthetic cholesterol-free diets containing coconut oil or butter or a conventional rabbit chow supplemented with cholesterol, were studied by preparative ultracentrifugation and electrophoresis. (1) All three diets elevated the total cholesterol level but only the coconut oil diet markedly increased the triglyceride (TG) content in addition. All ultracentrifugation fractions showed elevated cholesterol/TG ratios, and this was especially evident for the cholesterol diet. In the hyperlipidemic rabbits cholesterol was therefore mainly transported in lipoproteins with a changed lipid composition. (2) The lipid levels of the \"HDL\" fraction were more or less unaffected by the lipid concentration in whole serum. In the total serum cholesterol ranges 150-500 (coconut oil diet) and 100-300 mg/100 ml (cholesterol diet), most cholesterol was transported as \"LDL\" cholesterol. This latter fraction reached maximum cholesterol concentrations of about 350 (coconut oil diet) and 400 mg/100 ml (cholesterol diet) at total cholesterol levels of approximately 600 and 1200 mg/100 ml serum, respectively. At still higher levels of total cholesterol, the whole increment was concentrated to the \"VLDL\" fraction. (3) With semisynthetic diets in the whole cholesterol range 250-400 mg/100 ml it was possible, with respect to cholesterol, to induce fairly similar concentrations and distributions to those seen in man, with about 60% transported as \"LDL\", 30% as \"VLDL\" and 10% as \"HDL\" cholesterol with the coconut oil diet and 65%, 20% and 15%, respectively, with the butter diet. Such experimental conditions seem to be suitable for testing the hypocholesterolemic action of drugs intended for human hyperlipidemia Type II. (4) Compared with earlier investigations on rabbits, the present distribution study suggests that the degree of aortic lipid infiltration in cholesterol-fed rabbits is better related to the levels of \"LDL\" than to \"VLDL\" cholesterol."}
{"id": "PMID:1078400", "title": "Power changes converting aphakics from hard contact lenses to the B&L SOFLENS.", "content": "The authors refit 20 former hard lens aphakic eyes with the Bausch & Lomb SOFLENS. Ten patients required less soft lens power than the power of the hard contact lens formerly used; five patients required the same soft lenspower; and five patients required more power with the soft lens than with the previous hard lens used. These findings were different than what would have been expected on the basis of the Back-Vertex Power Chart distributed by Bausch & Lomb.", "contents": "Power changes converting aphakics from hard contact lenses to the B&L SOFLENS. The authors refit 20 former hard lens aphakic eyes with the Bausch & Lomb SOFLENS. Ten patients required less soft lens power than the power of the hard contact lens formerly used; five patients required the same soft lenspower; and five patients required more power with the soft lens than with the previous hard lens used. These findings were different than what would have been expected on the basis of the Back-Vertex Power Chart distributed by Bausch & Lomb."}
{"id": "PMID:1078401", "title": "A comparison of corneal topographical changes induced by corneal lens wear and keratoconus.", "content": "A distinction is made between keratoconus-like changes induced by physical pressures placed on the cornea by hard corneal lens wear and the simultaneous development of true keratoconus in contact lens wearers.", "contents": "A comparison of corneal topographical changes induced by corneal lens wear and keratoconus. A distinction is made between keratoconus-like changes induced by physical pressures placed on the cornea by hard corneal lens wear and the simultaneous development of true keratoconus in contact lens wearers."}
{"id": "PMID:1078402", "title": "Cholesterol: blood and tears.", "content": "The interrelationships of tear and blood cholesterol levels are explored with particular emphasis on the variables of age and obesity.", "contents": "Cholesterol: blood and tears. The interrelationships of tear and blood cholesterol levels are explored with particular emphasis on the variables of age and obesity."}
{"id": "PMID:1078403", "title": "Soft contact lens identification.", "content": "Measuring procedures used for hydrogel contact lenses are sufficiently difficult to make identification of a given soft lens annoyingly time-consuming and sometimes impossible. Etching a number or letter on the lens when it is received from the laboratory allows the practitioner to tell whether the proper lens is being worn, even while the lens is still on the patient's eye. A sharp pointed tool on a dry hydrogel lens produces this identifying number.", "contents": "Soft contact lens identification. Measuring procedures used for hydrogel contact lenses are sufficiently difficult to make identification of a given soft lens annoyingly time-consuming and sometimes impossible. Etching a number or letter on the lens when it is received from the laboratory allows the practitioner to tell whether the proper lens is being worn, even while the lens is still on the patient's eye. A sharp pointed tool on a dry hydrogel lens produces this identifying number."}
{"id": "PMID:1078404", "title": "Prepaid contact lens care for the hard lens patient.", "content": "The author presents his views as to why prepaid care is the best method of after care for contact lens patients. Detailed procedures are presented as to how to implement such a program. Legal justification for the use of prepaid care plans by the optometrist is given.", "contents": "Prepaid contact lens care for the hard lens patient. The author presents his views as to why prepaid care is the best method of after care for contact lens patients. Detailed procedures are presented as to how to implement such a program. Legal justification for the use of prepaid care plans by the optometrist is given."}
{"id": "PMID:1078405", "title": "Patient response to PMMA contact lenses.", "content": "This study was designed to determine patient response to hard (PMMA) contact lenses and evaluate how lens modifications and refitting affect success. The subjects of this study were 178 randomly selected contact lens patients fitted at the University of California School of Optometry. Six to nine months after the patients were examined and fitted, their response to contact lenses was evaluated using the standard of Sarver and Harris. 44.4% of the patients were successful with the initial lenses while an additional 35.4% of the patients became successful after appropriate lens changes were made. The majority of the changes made were to eliminate ocular tissue changes. Our results indicate that lens modifications or refittings are often necessary to achieve a successful response to contact lenses.", "contents": "Patient response to PMMA contact lenses. This study was designed to determine patient response to hard (PMMA) contact lenses and evaluate how lens modifications and refitting affect success. The subjects of this study were 178 randomly selected contact lens patients fitted at the University of California School of Optometry. Six to nine months after the patients were examined and fitted, their response to contact lenses was evaluated using the standard of Sarver and Harris. 44.4% of the patients were successful with the initial lenses while an additional 35.4% of the patients became successful after appropriate lens changes were made. The majority of the changes made were to eliminate ocular tissue changes. Our results indicate that lens modifications or refittings are often necessary to achieve a successful response to contact lenses."}
{"id": "PMID:1078519", "title": "Home humidifier thermophilic actinomycete isolates.", "content": "Twenty-seven thermophilic actinomycete isolates obtained from humidification systems or living-bedroom areas of individuals with suspected but unproved home environment-related respiratory disease were characterized morphologically and biochemically. All isolates were demonstrated to be members of the Thermoactinomyces genus. Two previously misidentified isolates were reclassified as a Thermoacetinomyces sp. Thus, all of our thermophilic actinomycete humidifier isolates studied to date have been identified as either Thermoactinomyces vulgaris or Thermoactinomyces sp. Large numbers of unidentified thermotolerant bacteria have also been isolated from virtually all residual humidifier water samples and their possible role in production of \"humidifier-associated\" respiratory disease is unknown.", "contents": "Home humidifier thermophilic actinomycete isolates. Twenty-seven thermophilic actinomycete isolates obtained from humidification systems or living-bedroom areas of individuals with suspected but unproved home environment-related respiratory disease were characterized morphologically and biochemically. All isolates were demonstrated to be members of the Thermoactinomyces genus. Two previously misidentified isolates were reclassified as a Thermoacetinomyces sp. Thus, all of our thermophilic actinomycete humidifier isolates studied to date have been identified as either Thermoactinomyces vulgaris or Thermoactinomyces sp. Large numbers of unidentified thermotolerant bacteria have also been isolated from virtually all residual humidifier water samples and their possible role in production of \"humidifier-associated\" respiratory disease is unknown."}
{"id": "PMID:1078522", "title": "[Autoimmune mechanisms and post-anesthetic hepatitis].", "content": "After a brief review of clinical, biological and immunological features of post anesthesia hepatitis and hepatic auto-immunity, the authors attempt to determine whether certain forms of post-anesthesia hepatitis can be placed under the heading of autoimmune liver disorders, the mechanisms by which auto-immunity can be induced and modulated and the practical implications of these concepts, namely with regard to detection of subjects at risk.", "contents": "[Autoimmune mechanisms and post-anesthetic hepatitis]. After a brief review of clinical, biological and immunological features of post anesthesia hepatitis and hepatic auto-immunity, the authors attempt to determine whether certain forms of post-anesthesia hepatitis can be placed under the heading of autoimmune liver disorders, the mechanisms by which auto-immunity can be induced and modulated and the practical implications of these concepts, namely with regard to detection of subjects at risk."}
{"id": "PMID:1078524", "title": "Progesterone-receptor mediation of chromatin RNA transcription in a cell-free system.", "content": "The chick oviduct progesterone receptor has been purified to homogeneity by affinity chromatography and its molecular action studied in vitro. The native receptor is a 200,000 MW dimer of two dissimilar 4S subunits with different intranuclear function. The receptors directly regulate RNA chain initiation sites in oviduct chromatin by interactions involving target tissue nuclear acceptor sites. There is a 1:1 correspondence between receptor \"acceptor\" sites and RNA sites. Only the dimer form of the receptor is active in vitro on chromatin templates. The study suggests a novel model for homone action that can be tested directly in this system.", "contents": "Progesterone-receptor mediation of chromatin RNA transcription in a cell-free system. The chick oviduct progesterone receptor has been purified to homogeneity by affinity chromatography and its molecular action studied in vitro. The native receptor is a 200,000 MW dimer of two dissimilar 4S subunits with different intranuclear function. The receptors directly regulate RNA chain initiation sites in oviduct chromatin by interactions involving target tissue nuclear acceptor sites. There is a 1:1 correspondence between receptor \"acceptor\" sites and RNA sites. Only the dimer form of the receptor is active in vitro on chromatin templates. The study suggests a novel model for homone action that can be tested directly in this system."}
{"id": "PMID:1078527", "title": "[Chemically defined diets as a diagnostic and therapeutic measure in food hypersensitivities].", "content": "Vivasorb proved successful as a diagnostic and therapeutic agent in a 78-year-old patient who had suffered from food allergy for three years. The alimentation with Vivasorb not only freed the patient of all her complaints, but also allowed the testing for the responsible allergens despite complete oral nutrition.", "contents": "[Chemically defined diets as a diagnostic and therapeutic measure in food hypersensitivities]. Vivasorb proved successful as a diagnostic and therapeutic agent in a 78-year-old patient who had suffered from food allergy for three years. The alimentation with Vivasorb not only freed the patient of all her complaints, but also allowed the testing for the responsible allergens despite complete oral nutrition."}
{"id": "PMID:1078528", "title": "[Myocardial infarct patients with and without intensive nutrition consultation over several years-- comparison of physiological and social variables].", "content": "After three years of experience with change of nutrition in 300 infarction patients of the H\u00f6henried long-term study (150 patients with intensive change of nutrition and 150 patients serving as control group) and 50 randomized study participants from the control group, the following results could be found: 1. In infarction patients, who underwent rehabilitation and follow-up examinations (group with change of nutrition), the increase of the P/S quotient causes no serum cholesterol and serum triglyceride decrease compared with the control group. 2. Alcohol and nicotine consumption cannot be further decreased by intensive nutrition consultation compared with the lasting effect of the comprehensive care. 3. As well, no further weight reduction (no less than the patient obtained during the rehabilitation phase) can be attained in the \"nutrition change\" and the control group. 4. From the \"nutrition change\" group more patients took up work again after the infarction compared with the control group, although age and social status are identical in both groups. These results are contradictory to nearly all other studies. We explain this difference by the fact that both groups were under comprehensive care. The care of the patient is a very decisive factor, which is the reason for keeping low the mentioned risk factors.", "contents": "[Myocardial infarct patients with and without intensive nutrition consultation over several years-- comparison of physiological and social variables]. After three years of experience with change of nutrition in 300 infarction patients of the H\u00f6henried long-term study (150 patients with intensive change of nutrition and 150 patients serving as control group) and 50 randomized study participants from the control group, the following results could be found: 1. In infarction patients, who underwent rehabilitation and follow-up examinations (group with change of nutrition), the increase of the P/S quotient causes no serum cholesterol and serum triglyceride decrease compared with the control group. 2. Alcohol and nicotine consumption cannot be further decreased by intensive nutrition consultation compared with the lasting effect of the comprehensive care. 3. As well, no further weight reduction (no less than the patient obtained during the rehabilitation phase) can be attained in the \"nutrition change\" and the control group. 4. From the \"nutrition change\" group more patients took up work again after the infarction compared with the control group, although age and social status are identical in both groups. These results are contradictory to nearly all other studies. We explain this difference by the fact that both groups were under comprehensive care. The care of the patient is a very decisive factor, which is the reason for keeping low the mentioned risk factors."}
{"id": "PMID:1078529", "title": "Quantitative studies on gastric emptying of Vivasorb.", "content": "The stomach clearance following the administration of 210 g Vivasorb in 700 ml water was ascertained in a 22-year-old healthy test subject. In the first hour, 50 g Vivasorb left the stomach, in the second hour 40 g and in the third and fourth hours, 30 g each. By doubling the dilution, the stomach clearance is not accelerated. An isocaloric glucose solution is cleared from the stomach just as rapidly as the Vivasorb solution.", "contents": "Quantitative studies on gastric emptying of Vivasorb. The stomach clearance following the administration of 210 g Vivasorb in 700 ml water was ascertained in a 22-year-old healthy test subject. In the first hour, 50 g Vivasorb left the stomach, in the second hour 40 g and in the third and fourth hours, 30 g each. By doubling the dilution, the stomach clearance is not accelerated. An isocaloric glucose solution is cleared from the stomach just as rapidly as the Vivasorb solution."}
{"id": "PMID:1078530", "title": "[Comparison of medical and surgical treatment of infantile hypothalamic obesity].", "content": "The jejunoileal bypass is, of all the current therapeutic possibilities, the only permanent method for the successful treatment of a patient with hypothalamic obesity. Pre-operatively, it is advisable, however, to reduce the body weight by exclusive alimentation with Vivonex, in order to improve lung function and diminish the operation risks. Putting a smaller section of the bowel at rest will prevent major weight loss, as well as more severe complications. The disturbances of the calcium and potassium metabolism and of liver function, which frequently occur after jejunoileal bypass operation, were not observed, when on both sides of the immobilised bowel section a section of small bowel 23 to 38 cm long was maintained in normal function.", "contents": "[Comparison of medical and surgical treatment of infantile hypothalamic obesity]. The jejunoileal bypass is, of all the current therapeutic possibilities, the only permanent method for the successful treatment of a patient with hypothalamic obesity. Pre-operatively, it is advisable, however, to reduce the body weight by exclusive alimentation with Vivonex, in order to improve lung function and diminish the operation risks. Putting a smaller section of the bowel at rest will prevent major weight loss, as well as more severe complications. The disturbances of the calcium and potassium metabolism and of liver function, which frequently occur after jejunoileal bypass operation, were not observed, when on both sides of the immobilised bowel section a section of small bowel 23 to 38 cm long was maintained in normal function."}
{"id": "PMID:1078532", "title": "[Questioning of diabetic patients on their knowledge of nutrition and dietetics as well as on their smoking habits].", "content": "100 diabetics were asked questions referring to the knowledge of nutrition to smoking habits. Most of the diabetics controlled their body weight regularly and were convinced that overweight may be dangerous to health. Despite this knowledge, more than half of the patients were overweight and did not know their individual ideal weight. Between the quality of dietary consultation and dietary behaviour of the patients a similar discrepancy existed: More than half of the questioned persons - especially males - had to be regarded as dietary failures. Patients with relatively low educational level came off worse. Fortunately, most of the patients were non-smokers. However, the dietary consultation practised at the moment seemed to have had no influence on the number of smokers. Consequently, for the training of the patients the following results: More than ever, the patients must be incited to normalize their body weight and they must be positively motivated in this regard as well as with regard to nicotine abuse. Behaviour therapy would certainly be desirable. Moreover, the patients should have a dietary consultation at least every second year. A dietary filled in for the patient personally must serve as the basis for the observance of the diet. Persons in whom diabetes mellitus was diagnosticated should receive consultation more quickly than up to now. Moreover, it would be useful to free the diet from the odium of an annoying and unimportant supplementation to medicamentous diabetes therapy. The study showed that continuous self-control of the own educational work is indispensable to guarantee a high standard of therapeutic efforts.", "contents": "[Questioning of diabetic patients on their knowledge of nutrition and dietetics as well as on their smoking habits]. 100 diabetics were asked questions referring to the knowledge of nutrition to smoking habits. Most of the diabetics controlled their body weight regularly and were convinced that overweight may be dangerous to health. Despite this knowledge, more than half of the patients were overweight and did not know their individual ideal weight. Between the quality of dietary consultation and dietary behaviour of the patients a similar discrepancy existed: More than half of the questioned persons - especially males - had to be regarded as dietary failures. Patients with relatively low educational level came off worse. Fortunately, most of the patients were non-smokers. However, the dietary consultation practised at the moment seemed to have had no influence on the number of smokers. Consequently, for the training of the patients the following results: More than ever, the patients must be incited to normalize their body weight and they must be positively motivated in this regard as well as with regard to nicotine abuse. Behaviour therapy would certainly be desirable. Moreover, the patients should have a dietary consultation at least every second year. A dietary filled in for the patient personally must serve as the basis for the observance of the diet. Persons in whom diabetes mellitus was diagnosticated should receive consultation more quickly than up to now. Moreover, it would be useful to free the diet from the odium of an annoying and unimportant supplementation to medicamentous diabetes therapy. The study showed that continuous self-control of the own educational work is indispensable to guarantee a high standard of therapeutic efforts."}
{"id": "PMID:1078533", "title": "[Diagnostic tests for hypersensitivity to halogen compounds].", "content": "The authors present a critical analysis of immunological tests used by some authors to establish the diagnosis of halothane-induced hepatitis. These tests include the lymphocyte transformation test (LTT), the leukocyte migration inhibition test (LMIT) and detection of auto-antibodies. It has not been possible for all investigators to reproduce the results of these tests. The inhibition of these tests is not specific for halothane since it is also observed after infectious and serum viral hepatitis.", "contents": "[Diagnostic tests for hypersensitivity to halogen compounds]. The authors present a critical analysis of immunological tests used by some authors to establish the diagnosis of halothane-induced hepatitis. These tests include the lymphocyte transformation test (LTT), the leukocyte migration inhibition test (LMIT) and detection of auto-antibodies. It has not been possible for all investigators to reproduce the results of these tests. The inhibition of these tests is not specific for halothane since it is also observed after infectious and serum viral hepatitis."}
{"id": "PMID:1078536", "title": "Direct and indirect pulp capping.", "content": "Cavity disinfection, indirect pulp capping, and direct pulp capping have been discussed in light of present day knowledge of the biology of the pulp. A format has been presented to the restorative dentist which has had long-standing clinical acceptance based on some scientific data and clinical evidence to substantiate the treatment radionale.", "contents": "Direct and indirect pulp capping. Cavity disinfection, indirect pulp capping, and direct pulp capping have been discussed in light of present day knowledge of the biology of the pulp. A format has been presented to the restorative dentist which has had long-standing clinical acceptance based on some scientific data and clinical evidence to substantiate the treatment radionale."}
{"id": "PMID:1078537", "title": "Some new studies of the wear behavior of ultrahigh molecular weight polyethylene.", "content": "The wear rate of ultrahigh molecular weight (UHMW) polyethylene over a long campaign of sliding distance is not constant. Both unfilled and graphite filled polyethylene show a high rate early, which reduces to a low value. The graphite-filled product shows a late stage increase in wear rate. Increased molding temperature reduces the molecular weight and increases the wear rate. Titanium articulating with UHMW polyethylene develops frequently an abnormally high wear rate with the generation of a black wear product.", "contents": "Some new studies of the wear behavior of ultrahigh molecular weight polyethylene. The wear rate of ultrahigh molecular weight (UHMW) polyethylene over a long campaign of sliding distance is not constant. Both unfilled and graphite filled polyethylene show a high rate early, which reduces to a low value. The graphite-filled product shows a late stage increase in wear rate. Increased molding temperature reduces the molecular weight and increases the wear rate. Titanium articulating with UHMW polyethylene develops frequently an abnormally high wear rate with the generation of a black wear product."}
{"id": "PMID:1078538", "title": "Identification and characterization of species of the family Bacteriodaceae by polyacrylamide gel electrophoresis.", "content": "Polyacrylamide gel electrophoresis was used to compare protein profiles of members of the family Bacteriodacceae. Cell-free extracts were prepared and the protein components separated by multistage polyacrylamide gel electrophoresis. The profiles were distinct and reproducible thus allowing identification of species, subspecies, and minor strain differences. Lyophilization of the cell-free extracts did not alter the major protein components. The results indicate that the techniques used may be useful in conjunction with conventional tests in identification at the species level.", "contents": "Identification and characterization of species of the family Bacteriodaceae by polyacrylamide gel electrophoresis. Polyacrylamide gel electrophoresis was used to compare protein profiles of members of the family Bacteriodacceae. Cell-free extracts were prepared and the protein components separated by multistage polyacrylamide gel electrophoresis. The profiles were distinct and reproducible thus allowing identification of species, subspecies, and minor strain differences. Lyophilization of the cell-free extracts did not alter the major protein components. The results indicate that the techniques used may be useful in conjunction with conventional tests in identification at the species level."}
{"id": "PMID:1078539", "title": "Sinus barotrauma in divers.", "content": "Sinus barotrauma is a common occupational disease of divers, with the incidence of descent barotrauma approximately double that of ascent. Pain chronologically associated with the change of pressure is the most dominant symptom and is seen in 92% of the cases presented for treatment. The majority complain of a frontal distribution of pain, with ethmoidal and maxillary being much less significant. Epistaxis is the second commonest symptom, and may be the sole symptom in some ascent cases. A history of recent or past sinus barotrauma or upper respiratory tract pathology is very common. Clinical examination supports the evidence of upper respiratory tract pathology in many cases. The radiological signs of abnormality were present in over three quarters of the cases examined. Of these the maxillary sinus was affected in most cases, the frontal in approximately one quarter and the ethmoidal in less than a fifth. The pathology was more commonly that of mucosal thickening, but in 12% of cases there was a fluid level. It is noted that although symptoms were predominantly frontal, x-ray changes were most often present in the maxillary sinuses.", "contents": "Sinus barotrauma in divers. Sinus barotrauma is a common occupational disease of divers, with the incidence of descent barotrauma approximately double that of ascent. Pain chronologically associated with the change of pressure is the most dominant symptom and is seen in 92% of the cases presented for treatment. The majority complain of a frontal distribution of pain, with ethmoidal and maxillary being much less significant. Epistaxis is the second commonest symptom, and may be the sole symptom in some ascent cases. A history of recent or past sinus barotrauma or upper respiratory tract pathology is very common. Clinical examination supports the evidence of upper respiratory tract pathology in many cases. The radiological signs of abnormality were present in over three quarters of the cases examined. Of these the maxillary sinus was affected in most cases, the frontal in approximately one quarter and the ethmoidal in less than a fifth. The pathology was more commonly that of mucosal thickening, but in 12% of cases there was a fluid level. It is noted that although symptoms were predominantly frontal, x-ray changes were most often present in the maxillary sinuses."}
{"id": "PMID:1078543", "title": "[Possibilities and limitations in the education of athe mentally handicapped (author's transl)].", "content": "The possibilities and limitations in the education of the mentally handicapped can today be defined in both medical and pedagogical terms. We think that the physician can create essential preconditions for optimal education of a mentally handicapped child by diagnostic retience, avoidance of negative suggestions, by observation of the possibilities which remain open, by concern for education as early as possible, by informing of special educational facilities and by emotional stabilization of the parents. This is the only way of attaining a successful upbringing with respect to a sufficient integration.", "contents": "[Possibilities and limitations in the education of athe mentally handicapped (author's transl)]. The possibilities and limitations in the education of the mentally handicapped can today be defined in both medical and pedagogical terms. We think that the physician can create essential preconditions for optimal education of a mentally handicapped child by diagnostic retience, avoidance of negative suggestions, by observation of the possibilities which remain open, by concern for education as early as possible, by informing of special educational facilities and by emotional stabilization of the parents. This is the only way of attaining a successful upbringing with respect to a sufficient integration."}
{"id": "PMID:1078540", "title": "[Comments on the infraspecific taxonomy of Sergentomyia minuta (Rondani, 1843), S. africana].", "content": "The authors discuss the systematic value of the cibarial armature of three members of the genus Sergentomyia. Subspecies could not be differentiated by the number of teeth, nor could members of the antennata and africana complexes. Moreover, from this character, the minuta complex along the Mediterranean coast of Spain appears to represent a typical \"cline\".", "contents": "[Comments on the infraspecific taxonomy of Sergentomyia minuta (Rondani, 1843), S. africana]. The authors discuss the systematic value of the cibarial armature of three members of the genus Sergentomyia. Subspecies could not be differentiated by the number of teeth, nor could members of the antennata and africana complexes. Moreover, from this character, the minuta complex along the Mediterranean coast of Spain appears to represent a typical \"cline\"."}
{"id": "PMID:1078546", "title": "Alternate-day prednisone therapy and human lymphocyte subpopulations.", "content": "The mechanisms and kinetics of the immunosuppressive effects of alternate-day prednisone were investigated in a group of patients with a variety of inflammatory diseases receiving a range of alternate-day prednisone doses from 5 to 120 mg. Total circulating lymphocyte and monocyte counts, as well as proportions of lymphocyte subpopulations defined  both by surface markers and by in vitro functional capacities, were studied. At 8 a. m. of the day on prednisone, just before drug administration, lymphocyte and monocyte counts, proportions of lymphocyte subpopulations, as well as in vitro lymphocyte blastogenic responses to various mitogenic and antigenic stimuli were normal. 4 h after the administration of prednisone, there was a profound lymphocytopenia and monocytopenia, with a differential depletion of thymus-derived lymphocytes as well as various functionally defined lymphocyte subpopulations. Lymphocyte kinetic studies using a radioactive chromium-labeled autologous lymphocytes showed that the lymphocytopenia was due predominantly to a transient depletion of the recirculating portion of the intravascular lymphocytepool. All these parameters returned to normal by 8 a.m. of the following day (off prednisone) and remained normal throughout the day. This very transient lymphocytopenia and monocytopenia after prednisone, with normal cell numbers, proportions, and functions throughout the remainder of the 2-day cycle, was associated with suppression of disease activity, yet did not affect cutaneous delayed hypersensitivity in these patients nor increase the likelihood of infectious complications. This drug-associated cyclic and transient monocytopenia and selective lymphocytopenia is best explained by a redistribution of recirculating lymphocytes to other body compartments, particularly the bone marrow.", "contents": "Alternate-day prednisone therapy and human lymphocyte subpopulations. The mechanisms and kinetics of the immunosuppressive effects of alternate-day prednisone were investigated in a group of patients with a variety of inflammatory diseases receiving a range of alternate-day prednisone doses from 5 to 120 mg. Total circulating lymphocyte and monocyte counts, as well as proportions of lymphocyte subpopulations defined  both by surface markers and by in vitro functional capacities, were studied. At 8 a. m. of the day on prednisone, just before drug administration, lymphocyte and monocyte counts, proportions of lymphocyte subpopulations, as well as in vitro lymphocyte blastogenic responses to various mitogenic and antigenic stimuli were normal. 4 h after the administration of prednisone, there was a profound lymphocytopenia and monocytopenia, with a differential depletion of thymus-derived lymphocytes as well as various functionally defined lymphocyte subpopulations. Lymphocyte kinetic studies using a radioactive chromium-labeled autologous lymphocytes showed that the lymphocytopenia was due predominantly to a transient depletion of the recirculating portion of the intravascular lymphocytepool. All these parameters returned to normal by 8 a.m. of the following day (off prednisone) and remained normal throughout the day. This very transient lymphocytopenia and monocytopenia after prednisone, with normal cell numbers, proportions, and functions throughout the remainder of the 2-day cycle, was associated with suppression of disease activity, yet did not affect cutaneous delayed hypersensitivity in these patients nor increase the likelihood of infectious complications. This drug-associated cyclic and transient monocytopenia and selective lymphocytopenia is best explained by a redistribution of recirculating lymphocytes to other body compartments, particularly the bone marrow."}
{"id": "PMID:1078547", "title": "Immunoblastic lymphadenopathy. A hyperimmune entity resembling Hodgkin's disease.", "content": "Immunoblastic lymphadenopathy, although it resembles Hodgkin's disease, is a distinct, hyperimmune disorder apparently of the B-cell system. In 32 cases, it was characterized by a morphologic triad: proliferation of arborizing small vessels; prominent immunoblastic proliferations; and amorphous acidophilic interstitial material. Clinically, it is manifested by fever, sweats, weight loss, occasionally a rash, generalized lymphadenopathy and often hepatosplenomegaly. There is a consistent polyclonal hyperglobulinemia and often hemolytic anemia. The course of the disease is usually progressive, with a median survival of 15 months in 18 fatal cases. The cellular proliferation appears benign morphologically in the pretherapy biopsies and in 10 of 12 available autopsy cases. In three cases the process evolved into a lymphoma of immunoblasts, immunoblastic sarcoma. The basic process appears to be a non-neoplastic hyperimmune proliferation of the B-cell system involving an exaggeration of lymphocyte transformation to immunoblasts and plasma cells that may be triggered by a hypersensitivity reaction to therapeutic agents.", "contents": "Immunoblastic lymphadenopathy. A hyperimmune entity resembling Hodgkin's disease. Immunoblastic lymphadenopathy, although it resembles Hodgkin's disease, is a distinct, hyperimmune disorder apparently of the B-cell system. In 32 cases, it was characterized by a morphologic triad: proliferation of arborizing small vessels; prominent immunoblastic proliferations; and amorphous acidophilic interstitial material. Clinically, it is manifested by fever, sweats, weight loss, occasionally a rash, generalized lymphadenopathy and often hepatosplenomegaly. There is a consistent polyclonal hyperglobulinemia and often hemolytic anemia. The course of the disease is usually progressive, with a median survival of 15 months in 18 fatal cases. The cellular proliferation appears benign morphologically in the pretherapy biopsies and in 10 of 12 available autopsy cases. In three cases the process evolved into a lymphoma of immunoblasts, immunoblastic sarcoma. The basic process appears to be a non-neoplastic hyperimmune proliferation of the B-cell system involving an exaggeration of lymphocyte transformation to immunoblasts and plasma cells that may be triggered by a hypersensitivity reaction to therapeutic agents."}
{"id": "PMID:1078548", "title": "Improvement of reduced left ventricular diastolic compliance in ischemic heart disease after successful coronary artery bypass surgery.", "content": "To determine the effects of myocardial revascularization on decreased left ventricular diastolic compliance consequent to chronic stable coronary artery disease, 15 patients with patent coronary artery bypass grafts (Group I) were compared with 8 patients with occluded grafts (Group II) studied before and after operation. In addition, eight normal patients served as controls. Left ventricular diastolic compliance was assessed by: (1) total observed diastolic compliance: stroke volume (V) related to left ventricular diastolic pressure (P) change (deltaV/deltaP) normalized for end-systolic volume; (2) left ventricular stiffness index of passive elastic modulus (a equals slope of deltaP/deltaV related to mean left ventricular diastolic pressure); and (3) fractional pattern of left ventricular filling. All patients had normal sinus rhythm and none had preoperative or postoperative mitral regurgitation. The two groups with coronary disease were well matched preoperatively for ventricular function, volumes, mass, segmental contraction and compliance. Ejection fraction increased in Group I (0.56 preoperatively to 0.65 postoperatively, P less than 0.05) but was unchanged in Group II (0.63 To 0.61, P greater 0.05). Postoperative indexes of left ventricular compliance improved in Group I: (1) 0.110 to 0.150 (P less 0.05); (2) 0.030 TO 0.019 (P less 0.05); and (3) 37 to 30 percent filling during last one third of diastole (P less 0.05). These indexes were unchanged postoperatively in Group II: (1) 0.109 to 0.102 (P greater 0.05); (2) 0.033 to 0.039 (P greater than 0.05); and (3) 36 to 41 percent (P greater 0.05). Compliance indexes were not altered (P greater 0.05) in a subset of seven patients in Group I with preoperative or intercurrent myocardial infarction. Thus, this investigation demonstrates the relatively reversible nature of abnormal left ventricular compliance after successful coronary artery bypass surgery in certain patients with ischemic heart disease.", "contents": "Improvement of reduced left ventricular diastolic compliance in ischemic heart disease after successful coronary artery bypass surgery. To determine the effects of myocardial revascularization on decreased left ventricular diastolic compliance consequent to chronic stable coronary artery disease, 15 patients with patent coronary artery bypass grafts (Group I) were compared with 8 patients with occluded grafts (Group II) studied before and after operation. In addition, eight normal patients served as controls. Left ventricular diastolic compliance was assessed by: (1) total observed diastolic compliance: stroke volume (V) related to left ventricular diastolic pressure (P) change (deltaV/deltaP) normalized for end-systolic volume; (2) left ventricular stiffness index of passive elastic modulus (a equals slope of deltaP/deltaV related to mean left ventricular diastolic pressure); and (3) fractional pattern of left ventricular filling. All patients had normal sinus rhythm and none had preoperative or postoperative mitral regurgitation. The two groups with coronary disease were well matched preoperatively for ventricular function, volumes, mass, segmental contraction and compliance. Ejection fraction increased in Group I (0.56 preoperatively to 0.65 postoperatively, P less than 0.05) but was unchanged in Group II (0.63 To 0.61, P greater 0.05). Postoperative indexes of left ventricular compliance improved in Group I: (1) 0.110 to 0.150 (P less 0.05); (2) 0.030 TO 0.019 (P less 0.05); and (3) 37 to 30 percent filling during last one third of diastole (P less 0.05). These indexes were unchanged postoperatively in Group II: (1) 0.109 to 0.102 (P greater 0.05); (2) 0.033 to 0.039 (P greater than 0.05); and (3) 36 to 41 percent (P greater 0.05). Compliance indexes were not altered (P greater 0.05) in a subset of seven patients in Group I with preoperative or intercurrent myocardial infarction. Thus, this investigation demonstrates the relatively reversible nature of abnormal left ventricular compliance after successful coronary artery bypass surgery in certain patients with ischemic heart disease."}
{"id": "PMID:1078552", "title": "Thymosin activity in patients with cellular immunodeficiency.", "content": "An extract of calf thymus, thymosin, induces an increase in percentage of T-cell rosettes when incubated in vitro with sheep erythrocytes and lymphocytes from patients with primary immunodeficiency diseases or viral illness. Precursor lymphocytes are required for this increase to occur. The percentage of T-cell rosettes, when they are normal, is not increased further upon incubation with thymosin. A patient with thymic hypoplasia and immunoglobulin synthesis was selected to receive thymosin in vivo when her T-cell rosettes had increased from 15 to 48 per cent after in vitro incubation with thymosin. During therapy, she clinically improved, the percentage of T-cell rosettes gradually increased to normal, and positive delayed hypersensitivity skin tests developed. Thymosin may be useful clinically for partial reconstitution of cellular immunity. An increased percentage of T-cell rosettes after incubation with thymosin in vitro may predict which patients will respond to thymosin therapy in vivo.", "contents": "Thymosin activity in patients with cellular immunodeficiency. An extract of calf thymus, thymosin, induces an increase in percentage of T-cell rosettes when incubated in vitro with sheep erythrocytes and lymphocytes from patients with primary immunodeficiency diseases or viral illness. Precursor lymphocytes are required for this increase to occur. The percentage of T-cell rosettes, when they are normal, is not increased further upon incubation with thymosin. A patient with thymic hypoplasia and immunoglobulin synthesis was selected to receive thymosin in vivo when her T-cell rosettes had increased from 15 to 48 per cent after in vitro incubation with thymosin. During therapy, she clinically improved, the percentage of T-cell rosettes gradually increased to normal, and positive delayed hypersensitivity skin tests developed. Thymosin may be useful clinically for partial reconstitution of cellular immunity. An increased percentage of T-cell rosettes after incubation with thymosin in vitro may predict which patients will respond to thymosin therapy in vivo."}
{"id": "PMID:1078553", "title": "Oral manifestations of Crohn's disease without demonstrable gastrointestinal lesions.", "content": "The literature relating to oral manifestations of Crohn's disease is reviewed and a case is reported in which clinical and histopathologic features of oral Crohn's disease are present without demonstrable gastrointestinal lesions. It is suggested that it is reasonable to make a firm diagnosis of the condition on this evidence alone and to monitor the patient for subsequent gastrointestinal symptoms, so that treatment can be instituted at an early stage.", "contents": "Oral manifestations of Crohn's disease without demonstrable gastrointestinal lesions. The literature relating to oral manifestations of Crohn's disease is reviewed and a case is reported in which clinical and histopathologic features of oral Crohn's disease are present without demonstrable gastrointestinal lesions. It is suggested that it is reasonable to make a firm diagnosis of the condition on this evidence alone and to monitor the patient for subsequent gastrointestinal symptoms, so that treatment can be instituted at an early stage."}
{"id": "PMID:1078554", "title": "Pancreatic disease.", "content": "New tests and test methods aid in the diagnosis of pancreatic disorders. Pancreatic carcinoma, especially, may have an improved prognosis with earlier detection as a result of refinements in arteriography, cytology, pancreatic radioisotopic scanning, and endoscopic retrograde cholangiopancreatography. Acute pancreatitis results most commonly from alcoholism, biliary tract disease, and trauma. Management is directed primarily at decreasing pancreatic exocrine secretion. Surgery is usually best avoided in the acute phase. Chronic pancreatitis is most often a result of recurrent attacks of acute pancreatitis. Diabetes and malassimilation become manifest as pancreatic destruction progresses. Management consists of replacement of pancreatic enzymes and diet supplements. Once chronic pancreatitis is established, surgery can only be directed at complications of the disease. Pancreatic ascites is usually associated with a break in the pancreatic ductal system. Ascites caused by trauma responds well to surgical intervention, but the alcoholic type is less amenable to treatment.", "contents": "Pancreatic disease. New tests and test methods aid in the diagnosis of pancreatic disorders. Pancreatic carcinoma, especially, may have an improved prognosis with earlier detection as a result of refinements in arteriography, cytology, pancreatic radioisotopic scanning, and endoscopic retrograde cholangiopancreatography. Acute pancreatitis results most commonly from alcoholism, biliary tract disease, and trauma. Management is directed primarily at decreasing pancreatic exocrine secretion. Surgery is usually best avoided in the acute phase. Chronic pancreatitis is most often a result of recurrent attacks of acute pancreatitis. Diabetes and malassimilation become manifest as pancreatic destruction progresses. Management consists of replacement of pancreatic enzymes and diet supplements. Once chronic pancreatitis is established, surgery can only be directed at complications of the disease. Pancreatic ascites is usually associated with a break in the pancreatic ductal system. Ascites caused by trauma responds well to surgical intervention, but the alcoholic type is less amenable to treatment."}
{"id": "PMID:1078555", "title": "The diagnosis and treatment of Raynaud's phenomenon.", "content": "Twenty-five patients with Raynaud's phenomenon, including 20 with associated diseases and five with primary Raynaud's disease, were evaluated with sequential determinations of digital temperature recovery time after cold exposure and magnification hand arteriography before and after cold exposure and before and after intra-arterial reserpine. A variety of immunologic screening tests were performed, and the clinical response to oral guanethidine of phenoxybenzamine was determined. Temperature recovery time was prolonged in all but two patients with Raynaud's phenomenon before treatment. Arteriography revealed luminal obstruction of variable degree in all but two patients with Raynaud's phenomenon but not in those with Raynaud's disease. Radiographic vasospasm was noted in all patients. Patients with Raynaud's symptoms had a markedly greater vasospastic response to cold exposure than did three control patients without Raynaud's symptoms. Arteriography 48 hours after intra-arterial reserpine repeat revealed decreased vasospasm and a decreased vasospastic response to cold in most patients. A variety of serum protein and serologic aberrations were detected, with only eight patients being free of immunologic abnormalities, Nineteen patients were treated with guanethidine alone, three with guanethidine-phenoxybenzamine combination, and one with phenoxybenzamine alone. Good or excellent clinical results were noted in 19 of the 23 patients treated, with an average follow-up to date of 12 months.", "contents": "The diagnosis and treatment of Raynaud's phenomenon. Twenty-five patients with Raynaud's phenomenon, including 20 with associated diseases and five with primary Raynaud's disease, were evaluated with sequential determinations of digital temperature recovery time after cold exposure and magnification hand arteriography before and after cold exposure and before and after intra-arterial reserpine. A variety of immunologic screening tests were performed, and the clinical response to oral guanethidine of phenoxybenzamine was determined. Temperature recovery time was prolonged in all but two patients with Raynaud's phenomenon before treatment. Arteriography revealed luminal obstruction of variable degree in all but two patients with Raynaud's phenomenon but not in those with Raynaud's disease. Radiographic vasospasm was noted in all patients. Patients with Raynaud's symptoms had a markedly greater vasospastic response to cold exposure than did three control patients without Raynaud's symptoms. Arteriography 48 hours after intra-arterial reserpine repeat revealed decreased vasospasm and a decreased vasospastic response to cold in most patients. A variety of serum protein and serologic aberrations were detected, with only eight patients being free of immunologic abnormalities, Nineteen patients were treated with guanethidine alone, three with guanethidine-phenoxybenzamine combination, and one with phenoxybenzamine alone. Good or excellent clinical results were noted in 19 of the 23 patients treated, with an average follow-up to date of 12 months."}
{"id": "PMID:1078556", "title": "Improvement of myocardial and other vital organ functions and metabolism with a simple method of pulsatile flow (IABP) during clinical cardiopulmonary bypass.", "content": "A simple, safe, and effective method of producing pulsatile flow during cardiopulmonary bypass (CPB) with intra-aortic balloon pumping (IABP) was used in 56 patients. No complications were associated with IABP. Myocardial metabolic studies, including coronary sinus lactates, mycoardial venous-arterial lactate differences, myocardial lactate extraction, and \"excess lactate\" were determined serially during the first hour of CPB. Changes in myocardial metabolism were statistically less abnormal in pulsatile flow (PF) patients when contrasted with a comparable group of nonpulsatile flow (NPF) patients. The changes were probably myocardial in origin since alterations in arterial lactates and lactate/pyruvate ratios were similar in both groups. In a comparable group of coronary bypass NPF patients, the percentage of left ventricular ejection fractions fell during the immediate postoperative period, whereas it rose in the PF group. Over-all body tissue injury (lactic dehydrogenase) and probably hepatocellular injury (serum glutamic oxalacetic transaminase) were less apparent in the PF patients. Postoperative low-cardiac-output syndrome did not occur in the PF patients and supportive drugs and diuretics were not needed. PF does not produce excessive hemolysis. This procedure may improve mortality rates by improving myocardial and other vital organ perfusion and by sustaining their function during weaning from CPB. This technique may prove superior to other forms of PF and is indicated in patients with severe left ventricular or other vital organ dysfunction and/or prolonged CPB.", "contents": "Improvement of myocardial and other vital organ functions and metabolism with a simple method of pulsatile flow (IABP) during clinical cardiopulmonary bypass. A simple, safe, and effective method of producing pulsatile flow during cardiopulmonary bypass (CPB) with intra-aortic balloon pumping (IABP) was used in 56 patients. No complications were associated with IABP. Myocardial metabolic studies, including coronary sinus lactates, mycoardial venous-arterial lactate differences, myocardial lactate extraction, and \"excess lactate\" were determined serially during the first hour of CPB. Changes in myocardial metabolism were statistically less abnormal in pulsatile flow (PF) patients when contrasted with a comparable group of nonpulsatile flow (NPF) patients. The changes were probably myocardial in origin since alterations in arterial lactates and lactate/pyruvate ratios were similar in both groups. In a comparable group of coronary bypass NPF patients, the percentage of left ventricular ejection fractions fell during the immediate postoperative period, whereas it rose in the PF group. Over-all body tissue injury (lactic dehydrogenase) and probably hepatocellular injury (serum glutamic oxalacetic transaminase) were less apparent in the PF patients. Postoperative low-cardiac-output syndrome did not occur in the PF patients and supportive drugs and diuretics were not needed. PF does not produce excessive hemolysis. This procedure may improve mortality rates by improving myocardial and other vital organ perfusion and by sustaining their function during weaning from CPB. This technique may prove superior to other forms of PF and is indicated in patients with severe left ventricular or other vital organ dysfunction and/or prolonged CPB."}
{"id": "PMID:1078571", "title": "Application of visually evoked response near the threshold of vision to objective measurement of dark adaptation.", "content": "The visually evoked response (VER) to dim lights with intensities within the scotopic or lower mesopic range increased in amplitude during the progressive dark adaptation. The VER amplitude vs. time curve resembled the psychophysical dark adaptation curve. The spectral sensitivity curve of the dark-adapted VER matched the C.I.E. scotopic sensitivity curve. The dark-adapted VER was abnormal in patients with retinitis pigmentosa and with congenital stationary night blindness and recordable in a patient with cone dysfunction. There was a close correlation between the VER threshold and subjective threshold of visual perception. The dark-adapted VER may be useful as an objective index of dark adaptation.", "contents": "Application of visually evoked response near the threshold of vision to objective measurement of dark adaptation. The visually evoked response (VER) to dim lights with intensities within the scotopic or lower mesopic range increased in amplitude during the progressive dark adaptation. The VER amplitude vs. time curve resembled the psychophysical dark adaptation curve. The spectral sensitivity curve of the dark-adapted VER matched the C.I.E. scotopic sensitivity curve. The dark-adapted VER was abnormal in patients with retinitis pigmentosa and with congenital stationary night blindness and recordable in a patient with cone dysfunction. There was a close correlation between the VER threshold and subjective threshold of visual perception. The dark-adapted VER may be useful as an objective index of dark adaptation."}
{"id": "PMID:1078573", "title": "Association of an ultraviolet irradiation sensitive cytoplasmic localization with the future dorsal side of the amphibian egg.", "content": "Drastic alterations in neural morphogenesis can be induced by ultraviolet irradiation of the amphibian egg. The target area of U.V. was determined either by direct irradiation of various regions of the egg or by comparing the area of the U.V. hit with the location of the dorsal lip. Axolotl eggs which displayed easily recognizable gray crescents were employed for the direct irradiation experiments and albino axolotl eggs were used for a comparison of the U.V. sensitivity of the animal and vegetal halves of the egg. Marked frog eggs were employed to determine the relationship between the sensitive area and the site of formation of the dorsal lip. It was concluded that the dorsal marginal zone of the fertilized, uncleaved egg was the most sensitive area of the egg. Ovarian homogenates provide a source of material for correcting, by microinjection, the effects of U.V.", "contents": "Association of an ultraviolet irradiation sensitive cytoplasmic localization with the future dorsal side of the amphibian egg. Drastic alterations in neural morphogenesis can be induced by ultraviolet irradiation of the amphibian egg. The target area of U.V. was determined either by direct irradiation of various regions of the egg or by comparing the area of the U.V. hit with the location of the dorsal lip. Axolotl eggs which displayed easily recognizable gray crescents were employed for the direct irradiation experiments and albino axolotl eggs were used for a comparison of the U.V. sensitivity of the animal and vegetal halves of the egg. Marked frog eggs were employed to determine the relationship between the sensitive area and the site of formation of the dorsal lip. It was concluded that the dorsal marginal zone of the fertilized, uncleaved egg was the most sensitive area of the egg. Ovarian homogenates provide a source of material for correcting, by microinjection, the effects of U.V."}
{"id": "PMID:1078574", "title": "Energy liberation and chemical change in frog skeletal muscle during single isometric tetanic contractions.", "content": "Recent data obtained from Rana temporaria sartorius muscles during an isometric tetanus indicate that the time-course of phosphocreatine (PC) splitting cannot account for the total energy (heat + work) liberation (Gilbert et al. 1971. J. Physiol. (Lond.) 218:)63). As this conclusion is important to an understanding of the chemical energetics of contraction, similar experments were performed on unpoisoned, oxygenated Rana pipiens sartorius muscles. The muscles were tetanized (isometrically) at 0 degrees C for 0.6, 1, or 5 s; metabolism was rapidly arrested by freezing the muscles with a specially designed hammer apparatus, and the frozen muscles were chemically analyzed. Comparable myothermal measurments were made on frogs from the same batch. Results of these experiments indicate: (a) The energy liberation parallels the PC and ATP breakdown with a proportionality constant of 10.7 kcal/mol; (b) comparably designed experiments with sartorius muscles of R. temporaria revealed that the ratio of energy liberation to PC splitting was significantly greater than that observed in R. pipiens sartorius muscles; (c) there is no systematic difference between experiments in which metabolism was arrested by the hammer apparatus and others using a conventional immersion technique.", "contents": "Energy liberation and chemical change in frog skeletal muscle during single isometric tetanic contractions. Recent data obtained from Rana temporaria sartorius muscles during an isometric tetanus indicate that the time-course of phosphocreatine (PC) splitting cannot account for the total energy (heat + work) liberation (Gilbert et al. 1971. J. Physiol. (Lond.) 218:)63). As this conclusion is important to an understanding of the chemical energetics of contraction, similar experments were performed on unpoisoned, oxygenated Rana pipiens sartorius muscles. The muscles were tetanized (isometrically) at 0 degrees C for 0.6, 1, or 5 s; metabolism was rapidly arrested by freezing the muscles with a specially designed hammer apparatus, and the frozen muscles were chemically analyzed. Comparable myothermal measurments were made on frogs from the same batch. Results of these experiments indicate: (a) The energy liberation parallels the PC and ATP breakdown with a proportionality constant of 10.7 kcal/mol; (b) comparably designed experiments with sartorius muscles of R. temporaria revealed that the ratio of energy liberation to PC splitting was significantly greater than that observed in R. pipiens sartorius muscles; (c) there is no systematic difference between experiments in which metabolism was arrested by the hammer apparatus and others using a conventional immersion technique."}
{"id": "PMID:1078575", "title": "Longitudinal impedance of single frog muscle fibers.", "content": "The longitudinal impedance of single skeletal muscle fibers has been measured from1 to 10,000 Hz in an oil gap apparatus which forces current to flow longitudinally down the fiber. The impedance observed is purely resistive in some fibers from the semitendinosus muscle and in two fibers from the sartorius muscle. In other fibers from the semitendinosus muscle a small phase shift is observed. The mean value of the maximum phase shift observed from all fibers is 1.07 degrees. The artifacts associated with the apparatus and method are examined theoretically and it is shown that one of the likely artifacts could account for the small phase observed. It is concluded that the longitudinal impedance of skeletal muscle fibers is essentially resistive and that little, if any, longitudinal current crosses the membranes of the sarcoplasmic reticulum.", "contents": "Longitudinal impedance of single frog muscle fibers. The longitudinal impedance of single skeletal muscle fibers has been measured from1 to 10,000 Hz in an oil gap apparatus which forces current to flow longitudinally down the fiber. The impedance observed is purely resistive in some fibers from the semitendinosus muscle and in two fibers from the sartorius muscle. In other fibers from the semitendinosus muscle a small phase shift is observed. The mean value of the maximum phase shift observed from all fibers is 1.07 degrees. The artifacts associated with the apparatus and method are examined theoretically and it is shown that one of the likely artifacts could account for the small phase observed. It is concluded that the longitudinal impedance of skeletal muscle fibers is essentially resistive and that little, if any, longitudinal current crosses the membranes of the sarcoplasmic reticulum."}
{"id": "PMID:1078577", "title": "An electrically evoked slow potential of the frog's retina. I. Properties of response.", "content": "1. Brief pulses of electrical current passed through the frog's eye cup from sclera to vitreous (inward current) evoked large, vitreous positive slow potentials of retinal origin. Brief pulses of outward current evoke no response. 2. This electrically evoked retinal response (EERG) increased sharply in amplitude as the strength of the stimulus was increased. 3. Strength-duration curves showed that the stimulating pulse was integrated with a time constant ranging from 14 to 36 ms. The time constant was a function of the amplitude chosen as a criterion. 4. The EERG was abolished by chemical agents which abolish the PII component of the ERG (KCl, NH3, aspartate). 5. The waveform of the EERG returned to the base line with exactly the same time course as the PII component of the ERG, both when the PII was evoked by flashes of light and when it was evoked by flashes of darkness. 6. These results can be explained in terms of the hypothesis that electrical stimuli act on the synaptic terminals of the photoreceptor cells.", "contents": "An electrically evoked slow potential of the frog's retina. I. Properties of response. 1. Brief pulses of electrical current passed through the frog's eye cup from sclera to vitreous (inward current) evoked large, vitreous positive slow potentials of retinal origin. Brief pulses of outward current evoke no response. 2. This electrically evoked retinal response (EERG) increased sharply in amplitude as the strength of the stimulus was increased. 3. Strength-duration curves showed that the stimulating pulse was integrated with a time constant ranging from 14 to 36 ms. The time constant was a function of the amplitude chosen as a criterion. 4. The EERG was abolished by chemical agents which abolish the PII component of the ERG (KCl, NH3, aspartate). 5. The waveform of the EERG returned to the base line with exactly the same time course as the PII component of the ERG, both when the PII was evoked by flashes of light and when it was evoked by flashes of darkness. 6. These results can be explained in terms of the hypothesis that electrical stimuli act on the synaptic terminals of the photoreceptor cells."}
{"id": "PMID:1078578", "title": "An electrically evoked slow potential of the frog's retina. II. Identification with PII component of electroretinogram.", "content": "1. To show conclusively that the electrically evoked retinal response (EERG) is in fact an electrically evoked component of the usual ERG, it is necessary to show that the two responses have the same intraretinal pattern of current generators. A method for determining when two responses have the same origin has been developed. This method utilizes measurements from a single microelectrode penetrating the retina. 2. The method was sensitive enough to detect differences in the origins of two responses when they were present. 3. The EERG had the same intraretinal origin as the PII component of the ERG, and thus is the PII component evoked by electricity rather than by light. 4. The hypothesis that electrical stimuli act on the synaptic terminals of the photoreceptor cells predicts that electrical current will evoke components of the ERG. The fact that the EERG is an electrically evoked component of the ERG fulfills that prediction.", "contents": "An electrically evoked slow potential of the frog's retina. II. Identification with PII component of electroretinogram. 1. To show conclusively that the electrically evoked retinal response (EERG) is in fact an electrically evoked component of the usual ERG, it is necessary to show that the two responses have the same intraretinal pattern of current generators. A method for determining when two responses have the same origin has been developed. This method utilizes measurements from a single microelectrode penetrating the retina. 2. The method was sensitive enough to detect differences in the origins of two responses when they were present. 3. The EERG had the same intraretinal origin as the PII component of the ERG, and thus is the PII component evoked by electricity rather than by light. 4. The hypothesis that electrical stimuli act on the synaptic terminals of the photoreceptor cells predicts that electrical current will evoke components of the ERG. The fact that the EERG is an electrically evoked component of the ERG fulfills that prediction."}
{"id": "PMID:1078579", "title": "Modification of the frog's electroretinogram by transretinal direct current.", "content": "1. Transretinal direct current modifies the amplitude and waveform of the frog's electroretinogram (ERG). Outward current (that flowing from vitreous to sclera) depresses the ERG while inward current enhances it. Outward current causes an apparent reversal of the polarity of the positive off-response (d-wave) of the ERG. 2. A true reversal does not actually occur. Rather, the outward current unmasks a negative off-response by depressing the positive off-response. 3. The positive off-response and the b-wave can be completely abolished by strong outward current, but cannot be reversed. 4. Treatment with ammonia abolishes the negative off-response, leaving a positive off-response which is affected by current in exactly the same way as the normal positive off-response. 5. Two possible mechanisms by which the current could be affecting the amplitude of the positive off-response would require that its amplitude be reversed for strong outward current. The absence of such reversal rejects these mechanisms.", "contents": "Modification of the frog's electroretinogram by transretinal direct current. 1. Transretinal direct current modifies the amplitude and waveform of the frog's electroretinogram (ERG). Outward current (that flowing from vitreous to sclera) depresses the ERG while inward current enhances it. Outward current causes an apparent reversal of the polarity of the positive off-response (d-wave) of the ERG. 2. A true reversal does not actually occur. Rather, the outward current unmasks a negative off-response by depressing the positive off-response. 3. The positive off-response and the b-wave can be completely abolished by strong outward current, but cannot be reversed. 4. Treatment with ammonia abolishes the negative off-response, leaving a positive off-response which is affected by current in exactly the same way as the normal positive off-response. 5. Two possible mechanisms by which the current could be affecting the amplitude of the positive off-response would require that its amplitude be reversed for strong outward current. The absence of such reversal rejects these mechanisms."}
{"id": "PMID:1078580", "title": "Neonatal craniopharyngioma. Case report.", "content": "The authors report a case of craniopharyngioma in a newborn infant. Marked hydrocephalus of the fetal head was recognized in the pelvimetric roentgenograms of the mother and massive suprasellar calcification was seen in the plain skull films.", "contents": "Neonatal craniopharyngioma. Case report. The authors report a case of craniopharyngioma in a newborn infant. Marked hydrocephalus of the fetal head was recognized in the pelvimetric roentgenograms of the mother and massive suprasellar calcification was seen in the plain skull films."}
{"id": "PMID:1078583", "title": "Alpha-1-antitrypsin deficiency, emphysema, cirrhosis, and intestinal mucosal atrophy.", "content": "An elderly man with long-standing pulmonary emphysema was seen with recent onset of steatorrhea and ascites. He had very low levels of serum alpha1-antitrypsin, hepatic cirrhosis, and intestinal mucosal atrophy. We believe this this combination of findings did not occur by chance.", "contents": "Alpha-1-antitrypsin deficiency, emphysema, cirrhosis, and intestinal mucosal atrophy. An elderly man with long-standing pulmonary emphysema was seen with recent onset of steatorrhea and ascites. He had very low levels of serum alpha1-antitrypsin, hepatic cirrhosis, and intestinal mucosal atrophy. We believe this this combination of findings did not occur by chance."}
{"id": "PMID:1078588", "title": "A clinical method for detecting subendocardial ischemia after cardiopulmonary bypass.", "content": "Subendocardial ischemia with consequent subendocardial necrosis is a frequent cause of death after cardiopulmonary bypass. The problem is caused by an inequity in the oxygen requirements of the subendocardium and the available blood supply. We have developed a means of detecting ischemia early in the postperfusion period. Using an analogue computer, we determine the endocardial viability ratio (EVR). This value may decrease before either systemic or central venous pressure changes. Thus the ratio can reflect early the danger of subendocardial ischemia. Another advantage is that equipment now common in coronary care units can be used to determine the EVR.", "contents": "A clinical method for detecting subendocardial ischemia after cardiopulmonary bypass. Subendocardial ischemia with consequent subendocardial necrosis is a frequent cause of death after cardiopulmonary bypass. The problem is caused by an inequity in the oxygen requirements of the subendocardium and the available blood supply. We have developed a means of detecting ischemia early in the postperfusion period. Using an analogue computer, we determine the endocardial viability ratio (EVR). This value may decrease before either systemic or central venous pressure changes. Thus the ratio can reflect early the danger of subendocardial ischemia. Another advantage is that equipment now common in coronary care units can be used to determine the EVR."}
{"id": "PMID:1078589", "title": "Direct selective myocardial revascularization by internal mammary artery-coronary vein anastomosis.", "content": "In this report, we shall describe our work with retrograde coronary perfusion, first in mongrel dogs and then in 6 patients. The left internal mammary artery (LIMA) is anastomosed to the left anterior descending coronary vein (LADV)  to provide myocardial perfusion. This procedure may be of great value in the treatment of patients who are not suitable candidates for direct coronary bypass grafting, i.e., those who have diffuse atherosclerosis, with poor or no runoff of the left anterior descending coronary artery (LADA),but who have satisfactory left ventricular contraction.", "contents": "Direct selective myocardial revascularization by internal mammary artery-coronary vein anastomosis. In this report, we shall describe our work with retrograde coronary perfusion, first in mongrel dogs and then in 6 patients. The left internal mammary artery (LIMA) is anastomosed to the left anterior descending coronary vein (LADV)  to provide myocardial perfusion. This procedure may be of great value in the treatment of patients who are not suitable candidates for direct coronary bypass grafting, i.e., those who have diffuse atherosclerosis, with poor or no runoff of the left anterior descending coronary artery (LADA),but who have satisfactory left ventricular contraction."}
{"id": "PMID:1078590", "title": "The rationale for surgery in preinfarction angina.", "content": "Of 123 patients with identical clinical criteria for preinfarction angina, 35 were treated medically and 88 surgically in a nonrandomized manner. There was no statistical difference between these two therapeutic groups in regard to age range, average age, sex distribution, number and distribution of prior myocardial infarction, and duration of chronic and acute anginal symptoms. With medical therapy, 32 sustained a myocardial infarction, and 14 (40 per cent) of these died within 1 month of hospital admission. Thirteen of 21 survivors or 37 per cent of the original group are asmptomatic on continuing medical therapy an average of 15 months after discharge. The surgical patients were studied by catherization and anigiography and underwent an early operation. Eight (9.4 per cent) demonstrated evidence of postoperative infarction, and 3 (3.4 per cent) died after the operation. Seventy-one (84 per cent) are completely asymptomatic and 6 are less symptomatic an average of 17 months after the operation. Actuarial analysis of the follow-up data reveals that the initial significant difference in survival between the two groups is maintained through the first 36 months. On the basis of this experience, we suggest that surgical intervention is the therapy of choice in patients with preinfarction angina.", "contents": "The rationale for surgery in preinfarction angina. Of 123 patients with identical clinical criteria for preinfarction angina, 35 were treated medically and 88 surgically in a nonrandomized manner. There was no statistical difference between these two therapeutic groups in regard to age range, average age, sex distribution, number and distribution of prior myocardial infarction, and duration of chronic and acute anginal symptoms. With medical therapy, 32 sustained a myocardial infarction, and 14 (40 per cent) of these died within 1 month of hospital admission. Thirteen of 21 survivors or 37 per cent of the original group are asmptomatic on continuing medical therapy an average of 15 months after discharge. The surgical patients were studied by catherization and anigiography and underwent an early operation. Eight (9.4 per cent) demonstrated evidence of postoperative infarction, and 3 (3.4 per cent) died after the operation. Seventy-one (84 per cent) are completely asymptomatic and 6 are less symptomatic an average of 17 months after the operation. Actuarial analysis of the follow-up data reveals that the initial significant difference in survival between the two groups is maintained through the first 36 months. On the basis of this experience, we suggest that surgical intervention is the therapy of choice in patients with preinfarction angina."}
{"id": "PMID:1078593", "title": "Suppression of lymphocyte transformation after aspirin ingestion.", "content": "To learn whether in vitro blastogenesis of lymphocytes is affected by in vivo salicylate ingestion, blood samples were obtained from 19 normal volunteers before and after therapeutic doses of aspirin. Lymphocyte transformation studies performed on these blood samples showed marked and statistically significant suppression of blastogenesis. Maximum suppression was observed in blood samples obtained 12 hours after the last aspirin ingestion. As compared to controls, samples obtained at this time showed mean blastogenic responses to phytohemagglutinin and pokeweed mitogens of 49 and 56 per cent respectively. No correlation could be demonstrated between the plasma salicylic acid level and the degree of suppression of blastogenesis. Aspirin ingestion altered neither the proportions of circulating T and B cells nor the viability of lymphocytes in culture.", "contents": "Suppression of lymphocyte transformation after aspirin ingestion. To learn whether in vitro blastogenesis of lymphocytes is affected by in vivo salicylate ingestion, blood samples were obtained from 19 normal volunteers before and after therapeutic doses of aspirin. Lymphocyte transformation studies performed on these blood samples showed marked and statistically significant suppression of blastogenesis. Maximum suppression was observed in blood samples obtained 12 hours after the last aspirin ingestion. As compared to controls, samples obtained at this time showed mean blastogenic responses to phytohemagglutinin and pokeweed mitogens of 49 and 56 per cent respectively. No correlation could be demonstrated between the plasma salicylic acid level and the degree of suppression of blastogenesis. Aspirin ingestion altered neither the proportions of circulating T and B cells nor the viability of lymphocytes in culture."}
{"id": "PMID:1078594", "title": "Quantitative determination of antibody in idiopathic thrombocytopenic purpura. Correlation of serum and platelet-bound antibody with clinical response.", "content": "We studied the clinical applicability of a recently developed technic that determines antiplatelet antibody directly on the platelet surface or in serum. The technic is a quantitative complement lysis-inhibition assay. Normal platelets have less than 0.4 pg of surface IgG. All patients with idiopathic thrombocytopenic purpura who were studied had greater than that value. Surface IgG was increased in inverse proportion to the platelet count. Surface levels of greater than 1.1 pg correlated with failure to respond to prednisone therapy. Incubation of normal serums with normal platelets did not increase surface IgG of such platelets, but the incubation with thrombocytopenic serums increased their surface IgG 0.5 to 100 times. The degree of increase did not predict response to treatment. However, quantitation of surface IgG of thrombocytopenic platelets was useful in predicting response to treatment.", "contents": "Quantitative determination of antibody in idiopathic thrombocytopenic purpura. Correlation of serum and platelet-bound antibody with clinical response. We studied the clinical applicability of a recently developed technic that determines antiplatelet antibody directly on the platelet surface or in serum. The technic is a quantitative complement lysis-inhibition assay. Normal platelets have less than 0.4 pg of surface IgG. All patients with idiopathic thrombocytopenic purpura who were studied had greater than that value. Surface IgG was increased in inverse proportion to the platelet count. Surface levels of greater than 1.1 pg correlated with failure to respond to prednisone therapy. Incubation of normal serums with normal platelets did not increase surface IgG of such platelets, but the incubation with thrombocytopenic serums increased their surface IgG 0.5 to 100 times. The degree of increase did not predict response to treatment. However, quantitation of surface IgG of thrombocytopenic platelets was useful in predicting response to treatment."}
{"id": "PMID:1078596", "title": "A community study of the relation of alpha1-antitrypsin levels to obstructive lung diseases.", "content": "Since it is still uncertain whether moderate degrees of alpha1-antitrypsin deficiency predispose to the development of lung disease, data obtained from a stratified random sample of white households in Tucson, Arizona, consisting of 2586 subjects over five years of age, were analyzed. No relation was found between serum alpha1-antitrypsin levels, measured as trypsin inhibitory capacity, and ventilatory function, respiratory symptomatology, or frequency of diagnosed pulmonary diseases even among cigarette smokers. The data indicate that an intermediate level of alpha1-antitrypsin deficiency (i.e., inhibitory capacity between 20 and 62 per cent of the population's mean value) is not an important risk factor for the development of chronic obstructive lung diseases. The data militate strongly against the use of any quantitative determination of alpha1-antitrypsin as a test to identify subjects with moderate deficiency for the purpose of predicting later development of chronic respiratory disorders. The rate of severe deficiency is so low as to make population screening for such subjects impractical.", "contents": "A community study of the relation of alpha1-antitrypsin levels to obstructive lung diseases. Since it is still uncertain whether moderate degrees of alpha1-antitrypsin deficiency predispose to the development of lung disease, data obtained from a stratified random sample of white households in Tucson, Arizona, consisting of 2586 subjects over five years of age, were analyzed. No relation was found between serum alpha1-antitrypsin levels, measured as trypsin inhibitory capacity, and ventilatory function, respiratory symptomatology, or frequency of diagnosed pulmonary diseases even among cigarette smokers. The data indicate that an intermediate level of alpha1-antitrypsin deficiency (i.e., inhibitory capacity between 20 and 62 per cent of the population's mean value) is not an important risk factor for the development of chronic obstructive lung diseases. The data militate strongly against the use of any quantitative determination of alpha1-antitrypsin as a test to identify subjects with moderate deficiency for the purpose of predicting later development of chronic respiratory disorders. The rate of severe deficiency is so low as to make population screening for such subjects impractical."}
{"id": "PMID:1078607", "title": "Partial purification and characterization of a lymphotoxic factor from multiple sclerosis serum.", "content": "A factor found in the serums of some patients with multiple sclerosis has been shown to have a toxic effect on the normal, mature lymphocyte. This lymphotoxic factor is a protein that is stable to heating at 56 degrees C for 3 hours. It is a nondialyzable material, with recovery of lymphotoxic activity following 30 days of dialysis. Elution of lymphotoxic serum through Sepadex G-100 demonstrates toxic activity closely associated with albumin. Purification by elution through DEAE-cellulose yielded lymphotoxic activity. The lymphotoxic factor was inactivated by standard ammonium sulfate precipitation. Analysis of the effect on distinctive cell types within the total lymphocyte population demonstrated that the T lymphocyte was particularly sensitive.", "contents": "Partial purification and characterization of a lymphotoxic factor from multiple sclerosis serum. A factor found in the serums of some patients with multiple sclerosis has been shown to have a toxic effect on the normal, mature lymphocyte. This lymphotoxic factor is a protein that is stable to heating at 56 degrees C for 3 hours. It is a nondialyzable material, with recovery of lymphotoxic activity following 30 days of dialysis. Elution of lymphotoxic serum through Sepadex G-100 demonstrates toxic activity closely associated with albumin. Purification by elution through DEAE-cellulose yielded lymphotoxic activity. The lymphotoxic factor was inactivated by standard ammonium sulfate precipitation. Analysis of the effect on distinctive cell types within the total lymphocyte population demonstrated that the T lymphocyte was particularly sensitive."}
{"id": "PMID:1078608", "title": "Use of potassium sorbate for treatment of fungal infections.", "content": "One hundred and twenty-two cases of vaginal fungal infections treated with potassium sorbate are presented. A new method of follow-up home application by means of vaginal tampons is tried. Relief of symptoms is prompt, and yeast organism disappear; the safety and superior efficacy of a strengthened (3%) solution is established. Treatment of fungal infections in males is also discussed.", "contents": "Use of potassium sorbate for treatment of fungal infections. One hundred and twenty-two cases of vaginal fungal infections treated with potassium sorbate are presented. A new method of follow-up home application by means of vaginal tampons is tried. Relief of symptoms is prompt, and yeast organism disappear; the safety and superior efficacy of a strengthened (3%) solution is established. Treatment of fungal infections in males is also discussed."}
{"id": "PMID:1078612", "title": "Meningitis due to an unusual type of Haemophilus influenzae.", "content": "An infant was admitted to hospital with suspected meningitis. Haemophilus influenzae type 'a' was isolated from cerebrospinal fluid (C.S.F.) and blood. Haemophilus meningitis due to types other than type 'b' is rare, and only a few due to type 'a' have so far been recorded. Investigations of the family are included together with a discussion of the implications for the diagnostic bacteriologist.", "contents": "Meningitis due to an unusual type of Haemophilus influenzae. An infant was admitted to hospital with suspected meningitis. Haemophilus influenzae type 'a' was isolated from cerebrospinal fluid (C.S.F.) and blood. Haemophilus meningitis due to types other than type 'b' is rare, and only a few due to type 'a' have so far been recorded. Investigations of the family are included together with a discussion of the implications for the diagnostic bacteriologist."}
{"id": "PMID:1078614", "title": "Unilateral ventricular obstruction due to infection.", "content": "A 14 year old male was hospitalized with pansinusitis complicated by intracranial spread of the infection. He developed a unilateral obstruction of the ventricular system of the foramen of Monro and a secondary mass effect revealed by radiological investigation.", "contents": "Unilateral ventricular obstruction due to infection. A 14 year old male was hospitalized with pansinusitis complicated by intracranial spread of the infection. He developed a unilateral obstruction of the ventricular system of the foramen of Monro and a secondary mass effect revealed by radiological investigation."}
{"id": "PMID:1078618", "title": "The use of hypnosis in hemophilia dental care.", "content": "The influence of emotional stress on the onset and control of bleeding episodes is a well-known fact. Oral surgical procedures are a common cause of severe states of anxiety in hemophiliacs. Anxiety has been shown to trigger and/or complicate an existing hemorrhagic episode in hemophiliacs. This is true in adults as well as children. Hypnosis, when applicable, has been found to be an excellent adjunct for the control of anxiety. It has been observed that in a relaxed and tranquil hemophiliac, his hemorrhagic tendency during and after surgery is considerably decreased. Induction to a hypnotic state depends on each individual's susceptibility, and therefore different approaches are used depending on the patient's knowledge of hypnosis. In addition to the control of fear, salivary secretions, pain, and capillary bleeding can well be brought under control during surgery and postoperatively by means of posthypnotic suggestions. Good oral hygiene habits such as daily brushing and flossing, so important as preventive measures for the prevention of caries and periodontal disease, can be reinforced through hypnotic suggestions. Preventive dentistry in hemophiliacs is of paramount importance.", "contents": "The use of hypnosis in hemophilia dental care. The influence of emotional stress on the onset and control of bleeding episodes is a well-known fact. Oral surgical procedures are a common cause of severe states of anxiety in hemophiliacs. Anxiety has been shown to trigger and/or complicate an existing hemorrhagic episode in hemophiliacs. This is true in adults as well as children. Hypnosis, when applicable, has been found to be an excellent adjunct for the control of anxiety. It has been observed that in a relaxed and tranquil hemophiliac, his hemorrhagic tendency during and after surgery is considerably decreased. Induction to a hypnotic state depends on each individual's susceptibility, and therefore different approaches are used depending on the patient's knowledge of hypnosis. In addition to the control of fear, salivary secretions, pain, and capillary bleeding can well be brought under control during surgery and postoperatively by means of posthypnotic suggestions. Good oral hygiene habits such as daily brushing and flossing, so important as preventive measures for the prevention of caries and periodontal disease, can be reinforced through hypnotic suggestions. Preventive dentistry in hemophiliacs is of paramount importance."}
{"id": "PMID:1078619", "title": "The therapeutic role of epsilon-aminocaproic acid (EACA) for dental extractions in hemophiliacs.", "content": "The results of a double-blind controlled trial, previously reported, showed that EACA is a useful adjunct to preoperative therapeutic concentrates of factor VIII or IX for dental extractions in hemophilia and Christmas disease. To estimate the amount of factor VIII and IX conserved in hemophiliacs receiving EACA for dental surgery compared with those not receiving EACA we have surveyed the usage of therapeutic materials in ten hemophilia centers in the U.S. and at Oxford. The amount of postoperative factor-VIII containing materials given to 20 U.S. hemophiliacs not receiving EACA averaged 11 062 units of factor-VIII activity per patient; 4,146 units for each of 22 U.S. patients receiving EACA; and 717 units for each of 56 patients at Oxford receiving EACA. Conservation of factor-IX-containing material was not as great. At Oxford 62.5% of patients receiving preoperative factor-VIII or -IX concentrates sufficient to raise the deficient factor to 50% of normal together with EACA, 24 g per day for ten days, required no postoperative therapeutic materials to control bleeding. The remainder required an average of two postoperative doses to control bleeding.", "contents": "The therapeutic role of epsilon-aminocaproic acid (EACA) for dental extractions in hemophiliacs. The results of a double-blind controlled trial, previously reported, showed that EACA is a useful adjunct to preoperative therapeutic concentrates of factor VIII or IX for dental extractions in hemophilia and Christmas disease. To estimate the amount of factor VIII and IX conserved in hemophiliacs receiving EACA for dental surgery compared with those not receiving EACA we have surveyed the usage of therapeutic materials in ten hemophilia centers in the U.S. and at Oxford. The amount of postoperative factor-VIII containing materials given to 20 U.S. hemophiliacs not receiving EACA averaged 11 062 units of factor-VIII activity per patient; 4,146 units for each of 22 U.S. patients receiving EACA; and 717 units for each of 56 patients at Oxford receiving EACA. Conservation of factor-IX-containing material was not as great. At Oxford 62.5% of patients receiving preoperative factor-VIII or -IX concentrates sufficient to raise the deficient factor to 50% of normal together with EACA, 24 g per day for ten days, required no postoperative therapeutic materials to control bleeding. The remainder required an average of two postoperative doses to control bleeding."}
{"id": "PMID:1078626", "title": "Benign small bowel tumor.", "content": "The clinical record and histologic sections of 84 cases of benign small bowel tumor are reviewed. Manifestations of systemic diseases, congenital anomalies, and lesions of either the ileocecal valve or periampullary region were excluded. In the same time span there were 96 small bowel malignancies. Clinical presentation, pathologic findings, management and result are compared to the collected published experience of about 2000 cases. There were 36 leiomyomas, 22 lipomas, 9 angiomas, 6 neurofibromas and 4 fibromas. Thirty-six men and 48 women were affected; the majority in their fifth and sixth decade. Seventy-eight were operative and 6 autopsy diagnoses. The most common symptom was obstruction (42%) followed by hemorrhage (34%) and pain (22%), relative frequency differing for the various specific tumors. There were rarely significant physical findings. A diagnosis of small bowel tumor was made radiologically in 30 patients. Because of the nonspecificity of other signs and symptoms, an acute awareness of the possibility of small bowel tumor is mandatory for preoperative anticipation of the diagnosis. Local resection was performed in all with no deaths or significant postoperative complications.", "contents": "Benign small bowel tumor. The clinical record and histologic sections of 84 cases of benign small bowel tumor are reviewed. Manifestations of systemic diseases, congenital anomalies, and lesions of either the ileocecal valve or periampullary region were excluded. In the same time span there were 96 small bowel malignancies. Clinical presentation, pathologic findings, management and result are compared to the collected published experience of about 2000 cases. There were 36 leiomyomas, 22 lipomas, 9 angiomas, 6 neurofibromas and 4 fibromas. Thirty-six men and 48 women were affected; the majority in their fifth and sixth decade. Seventy-eight were operative and 6 autopsy diagnoses. The most common symptom was obstruction (42%) followed by hemorrhage (34%) and pain (22%), relative frequency differing for the various specific tumors. There were rarely significant physical findings. A diagnosis of small bowel tumor was made radiologically in 30 patients. Because of the nonspecificity of other signs and symptoms, an acute awareness of the possibility of small bowel tumor is mandatory for preoperative anticipation of the diagnosis. Local resection was performed in all with no deaths or significant postoperative complications."}
{"id": "PMID:1078625", "title": "Labyrinthitis ossificans due to chronic otitis media.", "content": "Histopathology of two cases of unilateral labyrinthitis ossificans in the presence of bilateral chronic suppurative otitis media is presented. In both cases, ossification and fibrosis were limited to the scala tympani of the lower basal turn, apparently due to inflammation entering through the round window membrane. The round window was occluded by new bone and fibrous tissue presumably resulting in a conductive hearing loss in addition to the conductive loss due to middle ear lesions. Degeneration of the hair cells of the organ of Corti was limited to the lower basal turn where ossification and fibrosis occurred.", "contents": "Labyrinthitis ossificans due to chronic otitis media. Histopathology of two cases of unilateral labyrinthitis ossificans in the presence of bilateral chronic suppurative otitis media is presented. In both cases, ossification and fibrosis were limited to the scala tympani of the lower basal turn, apparently due to inflammation entering through the round window membrane. The round window was occluded by new bone and fibrous tissue presumably resulting in a conductive hearing loss in addition to the conductive loss due to middle ear lesions. Degeneration of the hair cells of the organ of Corti was limited to the lower basal turn where ossification and fibrosis occurred."}
{"id": "PMID:1078628", "title": "Frequency dependence of the frog skin impedance.", "content": "At frequencies between 20 Hz and 1 kHz the impedance locus of the isolated frog skin is circular; below 20 Hz the resistive component of the impedance is frequently greater than would be expected from extrapolation of the high-frequency locus. At frequencies greater than twice the highest frequency at which there are deviations from the circular locus the variation of impedance Z with angular frequency omega is closely described by the equation Z equals r1 plus r o/[1 plus (j omega tau)1-alpha], where j is square root--1, r 1 and r 0 are resistances, tau is a time constant and alpha a constant in the range 0.02-0.14.", "contents": "Frequency dependence of the frog skin impedance. At frequencies between 20 Hz and 1 kHz the impedance locus of the isolated frog skin is circular; below 20 Hz the resistive component of the impedance is frequently greater than would be expected from extrapolation of the high-frequency locus. At frequencies greater than twice the highest frequency at which there are deviations from the circular locus the variation of impedance Z with angular frequency omega is closely described by the equation Z equals r1 plus r o/[1 plus (j omega tau)1-alpha], where j is square root--1, r 1 and r 0 are resistances, tau is a time constant and alpha a constant in the range 0.02-0.14."}
{"id": "PMID:1078630", "title": "Electro-optical property of extremely stretched skinned muscle fibers.", "content": "Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V/cm) parallel to the fiber axis, provided that there was no overlap between thin and thick filaments. By use of a polarizing microscope, it was concluded that I-bands were mainly responsible for this intensity increase. By application of square pulses, the time course of the intensity increase and decay was followed. The analysis based on a simple model suggests: (a) Each thin filament has a permanent dipole movement and the movement directs from Z-bands to the free end of the thin filament. (b) The flexural rigidity of thin filaments is estimated to be similar to 3 with 10-17 dyn with cm-2. The present fibers will provide various applications in physiochemical studies of in vivo thin and thick filaments.", "contents": "Electro-optical property of extremely stretched skinned muscle fibers. Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V/cm) parallel to the fiber axis, provided that there was no overlap between thin and thick filaments. By use of a polarizing microscope, it was concluded that I-bands were mainly responsible for this intensity increase. By application of square pulses, the time course of the intensity increase and decay was followed. The analysis based on a simple model suggests: (a) Each thin filament has a permanent dipole movement and the movement directs from Z-bands to the free end of the thin filament. (b) The flexural rigidity of thin filaments is estimated to be similar to 3 with 10-17 dyn with cm-2. The present fibers will provide various applications in physiochemical studies of in vivo thin and thick filaments."}
{"id": "PMID:1078629", "title": "[Detection of longitudinal electric currents in frog nerves].", "content": "The data on transversal potential difference (V-x) in the sciatic nerve of a frog under the influence of the magnetic field are presented. The amplitude of the registered signal has a linear rise with an increase of the magnetic field tension. (H), while a change of (H) direction leads to a change of polarity of the signal (V-x). The presence of (V-x) is connected with the existence of the longitudinal directed stream of the light charged particles in the nerve.", "contents": "[Detection of longitudinal electric currents in frog nerves]. The data on transversal potential difference (V-x) in the sciatic nerve of a frog under the influence of the magnetic field are presented. The amplitude of the registered signal has a linear rise with an increase of the magnetic field tension. (H), while a change of (H) direction leads to a change of polarity of the signal (V-x). The presence of (V-x) is connected with the existence of the longitudinal directed stream of the light charged particles in the nerve."}
{"id": "PMID:1078637", "title": "Serotonin-sensitive adenylate cyclase activity of immature rat brain.", "content": "Cell-free preparations from superior and inferior colliculi of very young rats (1-3 days old) contained adenylate cyclase systems which were highly responsive to serotonin. The response to serotonin declined markedly during early development and was very low at maturity. Adenylate cyclase activity in the 10,000 times g particulate fraction from colliculi of newborn rats was significantly stimulated by 0.05 muM serotonin. Half-maximal activation was produced with less than 1 muM serotonin. Maximal stimulation of collicular adenylate cyclase was about 80% above basal enzyme activity and occurred with approximately 50 muM serotonin. Tryptamine and several derivatives of serotonin produced responses which were comparable to that obtained with serotonin; 5-methoxytryptamine was uniformly the most active compound tested. Norepinephrine or dopamine produced much smaller increases in adenylate cyclase activity. Stimulation of collicular adenylate cyclase by serotonin was significantly but incompletely blocked by serotonin antagonists, including d-lysergic acid diethylamide (d-LSD), 2-bromo-d-lysergic acid diethylamide, methysergide, 1-methyl-8 beta-carbobenzyloxy-aminomethyl-10 alpha-ergoline and cyproheptadine. Chlorpromazine also produced partial blockade. In contrast, l-lysergic acid diethylamide, haloperidol, propranolol, phenoxybenzamine and morphine were ineffective as serotonin blocking agents. Of the compounds which produced a partial blockage of serotonin action, d-LSD, cyproheptadine and chlorpromazine were themselves capable of stimulating adenylate cyclase activity. These results are consisent with the existence of multiple receptors in rat brain which are capable of interacting with indoleamines.", "contents": "Serotonin-sensitive adenylate cyclase activity of immature rat brain. Cell-free preparations from superior and inferior colliculi of very young rats (1-3 days old) contained adenylate cyclase systems which were highly responsive to serotonin. The response to serotonin declined markedly during early development and was very low at maturity. Adenylate cyclase activity in the 10,000 times g particulate fraction from colliculi of newborn rats was significantly stimulated by 0.05 muM serotonin. Half-maximal activation was produced with less than 1 muM serotonin. Maximal stimulation of collicular adenylate cyclase was about 80% above basal enzyme activity and occurred with approximately 50 muM serotonin. Tryptamine and several derivatives of serotonin produced responses which were comparable to that obtained with serotonin; 5-methoxytryptamine was uniformly the most active compound tested. Norepinephrine or dopamine produced much smaller increases in adenylate cyclase activity. Stimulation of collicular adenylate cyclase by serotonin was significantly but incompletely blocked by serotonin antagonists, including d-lysergic acid diethylamide (d-LSD), 2-bromo-d-lysergic acid diethylamide, methysergide, 1-methyl-8 beta-carbobenzyloxy-aminomethyl-10 alpha-ergoline and cyproheptadine. Chlorpromazine also produced partial blockade. In contrast, l-lysergic acid diethylamide, haloperidol, propranolol, phenoxybenzamine and morphine were ineffective as serotonin blocking agents. Of the compounds which produced a partial blockage of serotonin action, d-LSD, cyproheptadine and chlorpromazine were themselves capable of stimulating adenylate cyclase activity. These results are consisent with the existence of multiple receptors in rat brain which are capable of interacting with indoleamines."}
{"id": "PMID:1078638", "title": "Further observations of Fc receptors in human malignant tissue and normal lymphoid tissue.", "content": "Twenty human malignant solid tumors of various histologic types were tested for the presence of Fc receptor using cryostat sections or single cell suspensions of fresh tissue. Sheep erythrocytes sensitized by various amounts of rabbit IgG antibodies served as indicator cells (EA). All tumors possessed Fc receptor, but to varying degrees; eight reacted more strongly than normal spleen without any relation to histologic type. The tumors which gave the strongest reactions in sections also formed the highest percent of EA rosettes in suspensions, thus indicating surface localization of receptors. The reactions with spleen sections localized to the B cell and monocytic areas; the latter also showed high avidity in reactions with uncomplexed IgG. Rabbit antisera to tumors, spleen, and peripheral lymphocytes (polyvalent ALS) in inhibited the reactions, while a T-cell-specific ALS did not. Absorptions of the antisera with lymphocytes or tissue sediments of spleen and tumors removed the inhibiting activity, tissue sediments of muscle and kidney only reduced the titers. Again, results with spleen sections paralleled those obtained with tumor sections. Apparently, the tumor Fc receptor is very similar to the Fc receptors present in normal lymphoreticular tissues.", "contents": "Further observations of Fc receptors in human malignant tissue and normal lymphoid tissue. Twenty human malignant solid tumors of various histologic types were tested for the presence of Fc receptor using cryostat sections or single cell suspensions of fresh tissue. Sheep erythrocytes sensitized by various amounts of rabbit IgG antibodies served as indicator cells (EA). All tumors possessed Fc receptor, but to varying degrees; eight reacted more strongly than normal spleen without any relation to histologic type. The tumors which gave the strongest reactions in sections also formed the highest percent of EA rosettes in suspensions, thus indicating surface localization of receptors. The reactions with spleen sections localized to the B cell and monocytic areas; the latter also showed high avidity in reactions with uncomplexed IgG. Rabbit antisera to tumors, spleen, and peripheral lymphocytes (polyvalent ALS) in inhibited the reactions, while a T-cell-specific ALS did not. Absorptions of the antisera with lymphocytes or tissue sediments of spleen and tumors removed the inhibiting activity, tissue sediments of muscle and kidney only reduced the titers. Again, results with spleen sections paralleled those obtained with tumor sections. Apparently, the tumor Fc receptor is very similar to the Fc receptors present in normal lymphoreticular tissues."}
{"id": "PMID:1078639", "title": "The management of pulmonary metastases in children with osteogenic sarcoma with surgical resection combined with chemotherapy.", "content": "This report concerns a consecutive group of 10 children with metastatic osteogenic sarcoma treated with combined surgery and chemotherapy. Eight of 10 patients had thoracotomy prior to chemotherapy, and 2 after an initial trial of chemotherapy. Sequential chemotherapy was given to all patients and consisted of vincristine, high-dose methotrexate with citrovorum factor rescue, cyclophosphamide, and Adriamycin. Chemotherapy was continuously recycled for 1 year. The encouraging results obtained with these 10 patients are demonstrated with emphasis on the value of aggressive multidisciplinary therapy in salvaging patients with advanced metastatic osteogenic sarcoma.", "contents": "The management of pulmonary metastases in children with osteogenic sarcoma with surgical resection combined with chemotherapy. This report concerns a consecutive group of 10 children with metastatic osteogenic sarcoma treated with combined surgery and chemotherapy. Eight of 10 patients had thoracotomy prior to chemotherapy, and 2 after an initial trial of chemotherapy. Sequential chemotherapy was given to all patients and consisted of vincristine, high-dose methotrexate with citrovorum factor rescue, cyclophosphamide, and Adriamycin. Chemotherapy was continuously recycled for 1 year. The encouraging results obtained with these 10 patients are demonstrated with emphasis on the value of aggressive multidisciplinary therapy in salvaging patients with advanced metastatic osteogenic sarcoma."}
{"id": "PMID:1078640", "title": "Combination chemotherapy and radiation therapy in the treatment of metastatic osteogenic sarcoma.", "content": "Fourteen patients with 16 metastatic ostogenic sarcoma lesions were treated with high-dose methotrexate (HDMTX) with citrovorum factor rescue (CFR), adriamycin, and pulse high-dose cyclophosphamide combined with radiation therapy. Thirteen of 16 lesions responded. Responses consisted of relief of pain (6/6 patients) in bone lesions, roentgenographic and clinical evidence of decrease in the size of the bone lesions (6/7 patients), and a decrease in the size of pulmonary metastases (2/4 patients). The 2 patients whose pulmonary metastases responded to combined therapy developed pulmonary fibrosis and pneumonitis in the treated areas 3 months after radiation therapy (RT) (1400 and 1600 rads respectively). Of two bulky primary tumors that appeared to respond, both were ultimately found to contain viable tumor; a third less bulky primary tumor appeared to respond more completely. Three smaller metastatic bone lesions that were ultimately biopsied showed no evidence of active tumor. It is concluded that: 1) combination therapy (particularly HDMTX and RT) has an additive effect in controlling osteogenic sarcoma bone lesions, but bulky primary tumors cannot be completely eradicated; 2) although synergistic in treating osteogenic sarcoma, combination therapy can produce enhanced toxicity in surrounding normal lung tissue; and 3) combination therapy is of value in the palliative treatment of metastatic lesions other than that of lung, and in the treatment of small primary bone lesions. However, experience to date does not justify the delay in surgical ablation of a primary lesion in a child who presents without metastatic disease.", "contents": "Combination chemotherapy and radiation therapy in the treatment of metastatic osteogenic sarcoma. Fourteen patients with 16 metastatic ostogenic sarcoma lesions were treated with high-dose methotrexate (HDMTX) with citrovorum factor rescue (CFR), adriamycin, and pulse high-dose cyclophosphamide combined with radiation therapy. Thirteen of 16 lesions responded. Responses consisted of relief of pain (6/6 patients) in bone lesions, roentgenographic and clinical evidence of decrease in the size of the bone lesions (6/7 patients), and a decrease in the size of pulmonary metastases (2/4 patients). The 2 patients whose pulmonary metastases responded to combined therapy developed pulmonary fibrosis and pneumonitis in the treated areas 3 months after radiation therapy (RT) (1400 and 1600 rads respectively). Of two bulky primary tumors that appeared to respond, both were ultimately found to contain viable tumor; a third less bulky primary tumor appeared to respond more completely. Three smaller metastatic bone lesions that were ultimately biopsied showed no evidence of active tumor. It is concluded that: 1) combination therapy (particularly HDMTX and RT) has an additive effect in controlling osteogenic sarcoma bone lesions, but bulky primary tumors cannot be completely eradicated; 2) although synergistic in treating osteogenic sarcoma, combination therapy can produce enhanced toxicity in surrounding normal lung tissue; and 3) combination therapy is of value in the palliative treatment of metastatic lesions other than that of lung, and in the treatment of small primary bone lesions. However, experience to date does not justify the delay in surgical ablation of a primary lesion in a child who presents without metastatic disease."}
{"id": "PMID:1078641", "title": "Prolonged depression of cellular immunity in cured laryngopharyngeal cancer patients treated with radiation therapy.", "content": "Immune competence was evaluated in cured patients who had been treated by irradiation for carcinoma of the laryngopharynx, and compared with that of similar patients treated by surgery alone, and normal controls. Cellular immunity was determined by quantitation of in vitro phytohemagglutinin (PHA)-induced lymphocyte reactivity and peripheral blood thymus-dependent lymphocyte (T cell) levels. Humoral immunity was assessed by measurement of serum immunoglobulin levels and by the effect of serum on in vitro normal lymphocyte reactivity to PHA. In 21 patients who were studied for 4-23 years (mean 8.4) after surgical treatment alone for laryngopharyngeal carcinoma, neither cellular nor humoral immunity differed from that of 44 controls. In contrast, 14 patients who had been irradiated and subsequently cured for 4-15 years (mean 9.0) prior to evaluation displayed significantly impaired cellular immune competence when compared to normals and patients treated by surgery alone. Since previous determinations of the effects of radiation therapy have been in patients who received irratiation to the thymic region or large area of bone marrow, this study indicates that radiation therapy for cancer administered via portals that encompass a minimal area of the immune system may be associated with prolonged impairment of cellular immunity.", "contents": "Prolonged depression of cellular immunity in cured laryngopharyngeal cancer patients treated with radiation therapy. Immune competence was evaluated in cured patients who had been treated by irradiation for carcinoma of the laryngopharynx, and compared with that of similar patients treated by surgery alone, and normal controls. Cellular immunity was determined by quantitation of in vitro phytohemagglutinin (PHA)-induced lymphocyte reactivity and peripheral blood thymus-dependent lymphocyte (T cell) levels. Humoral immunity was assessed by measurement of serum immunoglobulin levels and by the effect of serum on in vitro normal lymphocyte reactivity to PHA. In 21 patients who were studied for 4-23 years (mean 8.4) after surgical treatment alone for laryngopharyngeal carcinoma, neither cellular nor humoral immunity differed from that of 44 controls. In contrast, 14 patients who had been irradiated and subsequently cured for 4-15 years (mean 9.0) prior to evaluation displayed significantly impaired cellular immune competence when compared to normals and patients treated by surgery alone. Since previous determinations of the effects of radiation therapy have been in patients who received irratiation to the thymic region or large area of bone marrow, this study indicates that radiation therapy for cancer administered via portals that encompass a minimal area of the immune system may be associated with prolonged impairment of cellular immunity."}
{"id": "PMID:1078642", "title": "The rationale for multiple drug chemotherapy in the treatment of osteogenic sarcoma.", "content": "Based on our prior experience in treating children with metastatic osteogenic sarcoma, a multidrug regimen was developed. Nine children with evaluable osteogenic sarcoma were treated with vincristine 1.5 mg/m2 on day 1, highdose methotrexate 200-300 mg/kg i.v. on day 2, with p.o. citrovorum factor \"rescue\" 9 mg every 6 hours x 12, followed in 2 weeks by cyclophosphamide 40 mg/kg i.v., then 2 weeks later Adriamycin 1.5 mg/kg/day x 2; in 2 weeks cyclophosphamide was repeated. After a 2-week rest, the 56-day cycle was repeated for a total period of 1 year. Oropharyngeal mucositis was the most frequent severe manifestation of gastrointestinal toxicity. Hematologic depression was mild to severe. Nine patients with clinically evaluable osteogenic sarcoma and no previous chemotherapeutic treatment were treated with this regimen. One patient had only a transient shrinkage in tumor mass, and one patient had no progression of multiple pulmonary and bone metastases for 16 months while on therapy. Of the remaining seven patients, all had clinically significant responses with tumor regression demonstrated for from 5 to 20+ months. Four of these patients (three presenting with primary tumor and pulmonary metastases) demonstrated regression of their primary tumor. In an attempt to increase the cure rate in osteogenic sarcoma, chemotherapy that has proven to be effective against metastatic osteogenic sarcoma should now be employed as prophylactic therapy, after amputation, at cancer treatment centers where it can be safely and effectively administered.", "contents": "The rationale for multiple drug chemotherapy in the treatment of osteogenic sarcoma. Based on our prior experience in treating children with metastatic osteogenic sarcoma, a multidrug regimen was developed. Nine children with evaluable osteogenic sarcoma were treated with vincristine 1.5 mg/m2 on day 1, highdose methotrexate 200-300 mg/kg i.v. on day 2, with p.o. citrovorum factor \"rescue\" 9 mg every 6 hours x 12, followed in 2 weeks by cyclophosphamide 40 mg/kg i.v., then 2 weeks later Adriamycin 1.5 mg/kg/day x 2; in 2 weeks cyclophosphamide was repeated. After a 2-week rest, the 56-day cycle was repeated for a total period of 1 year. Oropharyngeal mucositis was the most frequent severe manifestation of gastrointestinal toxicity. Hematologic depression was mild to severe. Nine patients with clinically evaluable osteogenic sarcoma and no previous chemotherapeutic treatment were treated with this regimen. One patient had only a transient shrinkage in tumor mass, and one patient had no progression of multiple pulmonary and bone metastases for 16 months while on therapy. Of the remaining seven patients, all had clinically significant responses with tumor regression demonstrated for from 5 to 20+ months. Four of these patients (three presenting with primary tumor and pulmonary metastases) demonstrated regression of their primary tumor. In an attempt to increase the cure rate in osteogenic sarcoma, chemotherapy that has proven to be effective against metastatic osteogenic sarcoma should now be employed as prophylactic therapy, after amputation, at cancer treatment centers where it can be safely and effectively administered."}
{"id": "PMID:1078649", "title": "Some important pitfalls in the diagnosis and treatment of bacterial meningitis in children.", "content": "Some of the common errors and problems of the physician who cares for the child with bacterial meningitis are: 1) misdiagnosis; 2) inappropriate handling of cerebrospinal fluid; 3) inadequate assessment of therapeutic responses; 4) inappropriate drug therapy; 5) improper treatment of seizures; 6) inadequate care of increased intracranial pressure; 7) inappropriate evaluation of persistent fever; 8) inappropriate fluid therapy; 9) inadequate investigation of persistent  neurologic deficit; 10) overvigorous treatment of subdural effusion; 11) inadequate assessment of neurologic or psychologic impairment; and 12) inadequate consideration of possible underlying defects.", "contents": "Some important pitfalls in the diagnosis and treatment of bacterial meningitis in children. Some of the common errors and problems of the physician who cares for the child with bacterial meningitis are: 1) misdiagnosis; 2) inappropriate handling of cerebrospinal fluid; 3) inadequate assessment of therapeutic responses; 4) inappropriate drug therapy; 5) improper treatment of seizures; 6) inadequate care of increased intracranial pressure; 7) inappropriate evaluation of persistent fever; 8) inappropriate fluid therapy; 9) inadequate investigation of persistent  neurologic deficit; 10) overvigorous treatment of subdural effusion; 11) inadequate assessment of neurologic or psychologic impairment; and 12) inadequate consideration of possible underlying defects."}
{"id": "PMID:1078651", "title": "Purification of human MM and ZZ alpha 1-antitrypsin.", "content": "Alpha -1-antitrypsin is the main protease inhibitor in human serum. Certain variants or phenotypes of this protein are associated with diseases of the liver and lung. The availability of purified alpha-1-antitrypsin of various phenotypes would be useful in studies exploring these associations. A two-step purification procedure is presented which involves affinity chromatography on isolubilized conconavalin A followed by preparative starch gel electrophoresis. This simple and rapid method has provided both MM and ZZ alpha-1-antitrypsin in highly pure and active form.", "contents": "Purification of human MM and ZZ alpha 1-antitrypsin. Alpha -1-antitrypsin is the main protease inhibitor in human serum. Certain variants or phenotypes of this protein are associated with diseases of the liver and lung. The availability of purified alpha-1-antitrypsin of various phenotypes would be useful in studies exploring these associations. A two-step purification procedure is presented which involves affinity chromatography on isolubilized conconavalin A followed by preparative starch gel electrophoresis. This simple and rapid method has provided both MM and ZZ alpha-1-antitrypsin in highly pure and active form."}
{"id": "PMID:1078653", "title": "[Immunosuppressive therapy in pure red cell aplasia].", "content": "Acquired erythroblastic aplasia of adults is a rare disease characterised by the absence of red cell precursors in the bone marrow. In a 34-year-old female patient the disease has been known for seven years. A partial remission had at first been achieved with glucocorticoids but regular transfusions had been necessary since 1971. Treatment with cyclophosphamide produced a remission which has lasted for over twelve months up to now. Histology of the bone marrow biopsy shows the appearance of active erythropoiesis after cyclophosphamide treatment which reflects well the clinical course.", "contents": "[Immunosuppressive therapy in pure red cell aplasia]. Acquired erythroblastic aplasia of adults is a rare disease characterised by the absence of red cell precursors in the bone marrow. In a 34-year-old female patient the disease has been known for seven years. A partial remission had at first been achieved with glucocorticoids but regular transfusions had been necessary since 1971. Treatment with cyclophosphamide produced a remission which has lasted for over twelve months up to now. Histology of the bone marrow biopsy shows the appearance of active erythropoiesis after cyclophosphamide treatment which reflects well the clinical course."}
{"id": "PMID:1078654", "title": "[The effect of aneurysm resection on left ventricular function].", "content": "The effect of successful aortocoronary artery bypass surgery on left ventricular (LV) function was studied by angiography in 13 patients with coronary-artery disease and resection of a ventricular aneurysm. The following were calculated from single-plane right anterior oblique LV cine-angiograms: LV end-diastolic volume (EDV), end-systolic volume (ESV), ejection fraction (EF), percentage shortening of the medial perpendicular short axis (deltaM), mean velocity shortening of the circumferential fibres (VCF). The changes in the five hemi-axes of the anterior LV wall, and five of the inferior wall were averaged to give a value for relative anterior wall motion (SMV) and inferior wall motion (SMH). Changes in apical motion (SMS) were calculated from the percentage changes in the base-to-apex axis. The following mean values (x plus or minus s) were obtained four months before and 11 months after aneurysmectomy: EDVI (ml/m2) 154 plus or minus 27 (126 plus or minus 23), ESVI (ml/m2) 107 plus or minus 28 (56 plus or minus 15), SVI (ML/M2) 46.5 plus or minus 12.7 (69.9 plus or minus 23.1), EF (%) 32.6 plus or minus 8.0 (54.6 plus or minus 12.0), SMV (%) 6.1 plus or minus 10.0 (25.0 plus or minus 13.4), SMH (%) 25.9 plus or minus 13.1 (31.2 plus or minus 11.1), SMS (%) 5.1 PLus or minus 3.4 (13.1 plus or minus 4.2). These results indicated significant improvement in ventricular pumping action and wall motion after aneurysm resection and aortacoronary bypass surgery in patients with coronary-artery disease and severe cardiac failure.", "contents": "[The effect of aneurysm resection on left ventricular function]. The effect of successful aortocoronary artery bypass surgery on left ventricular (LV) function was studied by angiography in 13 patients with coronary-artery disease and resection of a ventricular aneurysm. The following were calculated from single-plane right anterior oblique LV cine-angiograms: LV end-diastolic volume (EDV), end-systolic volume (ESV), ejection fraction (EF), percentage shortening of the medial perpendicular short axis (deltaM), mean velocity shortening of the circumferential fibres (VCF). The changes in the five hemi-axes of the anterior LV wall, and five of the inferior wall were averaged to give a value for relative anterior wall motion (SMV) and inferior wall motion (SMH). Changes in apical motion (SMS) were calculated from the percentage changes in the base-to-apex axis. The following mean values (x plus or minus s) were obtained four months before and 11 months after aneurysmectomy: EDVI (ml/m2) 154 plus or minus 27 (126 plus or minus 23), ESVI (ml/m2) 107 plus or minus 28 (56 plus or minus 15), SVI (ML/M2) 46.5 plus or minus 12.7 (69.9 plus or minus 23.1), EF (%) 32.6 plus or minus 8.0 (54.6 plus or minus 12.0), SMV (%) 6.1 plus or minus 10.0 (25.0 plus or minus 13.4), SMH (%) 25.9 plus or minus 13.1 (31.2 plus or minus 11.1), SMS (%) 5.1 PLus or minus 3.4 (13.1 plus or minus 4.2). These results indicated significant improvement in ventricular pumping action and wall motion after aneurysm resection and aortacoronary bypass surgery in patients with coronary-artery disease and severe cardiac failure."}
{"id": "PMID:1078655", "title": "Melanophore stimulating hormone (MSH) release: inhibition by cytochalasin B and \"stimulation\" by dimethyl sulfoxide.", "content": "Incubation of rat or frog pituitaries in cytochalasin B (10-4 minus 10-8 g/ml) for 2, 4 or 6 hr produces a time-dependent inhibition of MSH release. This inhibition is reversible and, again, time-dependent since longer incubations of pituitaries returned to normal media result in greater hormone secretion. Cytochalasin B inhibits MSH release even at a high concentration (10%) of dimethylsulfoxide (DMSO) which, itself, is \"stimulatory\" to hormone release. MSH release in high DMSO concentration is also inhibited by ouabain or by the absence of Ca2+ from the medium. In amphibian Ringer enriched with glucose, and in Medium 199, cytochalasin B fails to inhibit the release of MSH from the frog pituitary. Colchicine, vinblastine, and vincristine (10-3 minus 10-6 g/ml) were without effect on MSH release. These data suggest a possible role for microfilaments in the control of MSH release but fail to provide information on the exact nature of the mechanisms involved.", "contents": "Melanophore stimulating hormone (MSH) release: inhibition by cytochalasin B and \"stimulation\" by dimethyl sulfoxide. Incubation of rat or frog pituitaries in cytochalasin B (10-4 minus 10-8 g/ml) for 2, 4 or 6 hr produces a time-dependent inhibition of MSH release. This inhibition is reversible and, again, time-dependent since longer incubations of pituitaries returned to normal media result in greater hormone secretion. Cytochalasin B inhibits MSH release even at a high concentration (10%) of dimethylsulfoxide (DMSO) which, itself, is \"stimulatory\" to hormone release. MSH release in high DMSO concentration is also inhibited by ouabain or by the absence of Ca2+ from the medium. In amphibian Ringer enriched with glucose, and in Medium 199, cytochalasin B fails to inhibit the release of MSH from the frog pituitary. Colchicine, vinblastine, and vincristine (10-3 minus 10-6 g/ml) were without effect on MSH release. These data suggest a possible role for microfilaments in the control of MSH release but fail to provide information on the exact nature of the mechanisms involved."}
{"id": "PMID:1078656", "title": "A serum melatonin rhythm in chickens and its abolition by pinealectomy.", "content": "Melatonin, a suspected hormone of the pineal organ, was extracted from chicken serum and quantified by the Rana pipiens bioassay. When chickens were housed in an alternating light-dark photoperiod, serum melatonin was usually not detected during the light phase. Serum melatonin rose within 1 hr after the lights were extinguished, plateaued around midnight and fell before the lights were turned on. Serum melatonin was not detected at any time of day in pinealectomized chickens, suggesting that the pineal is neccessary for, and may be the source of, circulating melatonin.", "contents": "A serum melatonin rhythm in chickens and its abolition by pinealectomy. Melatonin, a suspected hormone of the pineal organ, was extracted from chicken serum and quantified by the Rana pipiens bioassay. When chickens were housed in an alternating light-dark photoperiod, serum melatonin was usually not detected during the light phase. Serum melatonin rose within 1 hr after the lights were extinguished, plateaued around midnight and fell before the lights were turned on. Serum melatonin was not detected at any time of day in pinealectomized chickens, suggesting that the pineal is neccessary for, and may be the source of, circulating melatonin."}
{"id": "PMID:1078670", "title": "Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase.", "content": "The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either substrate is unaffected by the presence of the other. dADP, an analogue of ADP product, is a competitive inhibitor with respect to Pi and polynucleotidy. (Ap)tA-cyclic-p is a competitive inhibitor with respect to Pi. The results are almost identical with both primer-independent (Form-I) and primer-dependent (Form-T) enzymes, although the various kinetic constants differ. On the vasis of these data a rapid equilibrium random Bi Bi mechanism is proposed. The demonstration of two different inhibitor constants for dADP and the difference between the Michaelis and the inhibitor constant for polyadenylic acid in polynucleotide phosphorolysis indicate at least two binding sites for polyadenylic acid and dADP on M. luteus polynucleotide phosphorylase. Its is suggested that in the phosphorolysis of long chain polymers the second binding site permits the polynucleotide to snap right back into position after removal of I mononucleotide unit and thus leads to the observed processive degradation. A general discussion of oligonucleotide and polynucleotide phosphorolysis and the differences between Form-I and Form-T enzymes in de novo synthesis and degradation of polynucleotides is presented.", "contents": "Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase. The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either substrate is unaffected by the presence of the other. dADP, an analogue of ADP product, is a competitive inhibitor with respect to Pi and polynucleotidy. (Ap)tA-cyclic-p is a competitive inhibitor with respect to Pi. The results are almost identical with both primer-independent (Form-I) and primer-dependent (Form-T) enzymes, although the various kinetic constants differ. On the vasis of these data a rapid equilibrium random Bi Bi mechanism is proposed. The demonstration of two different inhibitor constants for dADP and the difference between the Michaelis and the inhibitor constant for polyadenylic acid in polynucleotide phosphorolysis indicate at least two binding sites for polyadenylic acid and dADP on M. luteus polynucleotide phosphorylase. Its is suggested that in the phosphorolysis of long chain polymers the second binding site permits the polynucleotide to snap right back into position after removal of I mononucleotide unit and thus leads to the observed processive degradation. A general discussion of oligonucleotide and polynucleotide phosphorolysis and the differences between Form-I and Form-T enzymes in de novo synthesis and degradation of polynucleotides is presented."}
{"id": "PMID:1078668", "title": "Surgical therapy in acute ischemic syndromes.", "content": "Despite increasing enthusiasm about the treatment of symptomatic angina pectoris by direct revascularization surgery, there appropriately continues to be concern about the effects of such therapy. While it is generally accepted that surgery is effective in relieving anginal pain in upwards of 80% of patients undergoing aortocoronary bypass, reservations focus on the possibilities that such therapy may increase the incidence of infarction (postoperative), accelerate the atherosclerotic process, and shorten longevity, primarily because of the increased early operative mortality. While these contentions may or may not be true for the majority of patients who undergo such therapy, there is accumulating experience that in certain well-defined subsets, surgery does favorably affect the prognosis of the disease. Patients with preinfarction angina constitute one such subset of patients with coronary atherosclerosis. The all-inclusive surgical mortality for 106 consecutive cases was 3.8%, and 86% of the survivors are asymptomatic. Actuarial analysis of follow-up data reveals that this survival rate is essentially constant through the first 36 months after surgery. On the basis of this experience we feel that patients with preinfarction angina present a therapeutic opportunity in which the ideal goal of preventive medical care can be achieved by early identification, study and surgery.", "contents": "Surgical therapy in acute ischemic syndromes. Despite increasing enthusiasm about the treatment of symptomatic angina pectoris by direct revascularization surgery, there appropriately continues to be concern about the effects of such therapy. While it is generally accepted that surgery is effective in relieving anginal pain in upwards of 80% of patients undergoing aortocoronary bypass, reservations focus on the possibilities that such therapy may increase the incidence of infarction (postoperative), accelerate the atherosclerotic process, and shorten longevity, primarily because of the increased early operative mortality. While these contentions may or may not be true for the majority of patients who undergo such therapy, there is accumulating experience that in certain well-defined subsets, surgery does favorably affect the prognosis of the disease. Patients with preinfarction angina constitute one such subset of patients with coronary atherosclerosis. The all-inclusive surgical mortality for 106 consecutive cases was 3.8%, and 86% of the survivors are asymptomatic. Actuarial analysis of follow-up data reveals that this survival rate is essentially constant through the first 36 months after surgery. On the basis of this experience we feel that patients with preinfarction angina present a therapeutic opportunity in which the ideal goal of preventive medical care can be achieved by early identification, study and surgery."}
{"id": "PMID:1078671", "title": "Distribution of microtubules and microfilaments in developing vestibular sensory epithelium of mouse otocysts grown in vitro.", "content": "Otocysts explanted from 12th-gestation-day mice and maintained in organ culture under went a series of developmental changes which paralleled those that occurred in vivo and which resulted in the formation of a sensory epithelium of the vestibular type. At the time of explantation presumptive vestibular sensory epithelium consisted of cells that were undifferentiated, pseudostratified and rapidly proliferating. The only microtubules present were those of the mitotic apparatus. After 4 days of in vitro development cells comprising the presumptive vestibular sensory epithelium were less pseudostratified and more elongate; their nuclei had assumed a basal orientation and there was a clear maginal velum. Longitudinally oriented cytoplasmic microtubules were present at the apices of some cells; they were often grouped around a centriole which may have served as a nucleation centre for their assembly. After 7 days of in vitro development mitosis had ceased and supporting cells had innervated hair cells were present: both types of cells were always longer than they were broad and were often highly asymmetrical. Hair cells were flask- or columnar-shaped, with a nucleus situated in the basal third of the cell. Most mitochondria in hair cells were located in the apical third of the cell. The same distribution of mitochondria and nuclei was evident in supporting cells. Microtubules occurred throughout the length of the supporting cell and were always parallel to its longitudinal axis. In hair cells microtubules were more frequent than in supporting cells: the majority were parallel to the longitudinal axis of the cell but there were two exceptions. First, at the apex of hair cells some microtubules were oriented transversely and diagnonally: these were probably involved in the development and maintenance of the constricted apex of these cells. Secondly, microtubules appeared to be randomly arranged in the narrow region of the cytoplasm between the ventral surface of the nucleus and the base of the hair cells. Microfilaments were confined to the basal third of hair cells where their orientation paralleled that of microtubules. The possible functions of microtubules and microfilaments in the development of hair cells and supporting cells of the mouse vestibular epithelium are discussed.", "contents": "Distribution of microtubules and microfilaments in developing vestibular sensory epithelium of mouse otocysts grown in vitro. Otocysts explanted from 12th-gestation-day mice and maintained in organ culture under went a series of developmental changes which paralleled those that occurred in vivo and which resulted in the formation of a sensory epithelium of the vestibular type. At the time of explantation presumptive vestibular sensory epithelium consisted of cells that were undifferentiated, pseudostratified and rapidly proliferating. The only microtubules present were those of the mitotic apparatus. After 4 days of in vitro development cells comprising the presumptive vestibular sensory epithelium were less pseudostratified and more elongate; their nuclei had assumed a basal orientation and there was a clear maginal velum. Longitudinally oriented cytoplasmic microtubules were present at the apices of some cells; they were often grouped around a centriole which may have served as a nucleation centre for their assembly. After 7 days of in vitro development mitosis had ceased and supporting cells had innervated hair cells were present: both types of cells were always longer than they were broad and were often highly asymmetrical. Hair cells were flask- or columnar-shaped, with a nucleus situated in the basal third of the cell. Most mitochondria in hair cells were located in the apical third of the cell. The same distribution of mitochondria and nuclei was evident in supporting cells. Microtubules occurred throughout the length of the supporting cell and were always parallel to its longitudinal axis. In hair cells microtubules were more frequent than in supporting cells: the majority were parallel to the longitudinal axis of the cell but there were two exceptions. First, at the apex of hair cells some microtubules were oriented transversely and diagnonally: these were probably involved in the development and maintenance of the constricted apex of these cells. Secondly, microtubules appeared to be randomly arranged in the narrow region of the cytoplasm between the ventral surface of the nucleus and the base of the hair cells. Microfilaments were confined to the basal third of hair cells where their orientation paralleled that of microtubules. The possible functions of microtubules and microfilaments in the development of hair cells and supporting cells of the mouse vestibular epithelium are discussed."}
{"id": "PMID:1078669", "title": "Revascularization via healthy myocardial arteriolar networks compared with that through diseased surface coronary arteries.", "content": "Myocardial mammary artery implants revascularize via the undiseased coronary microcirculation. Such implants, followed for up to 20 years, showed no atherosclerosis, while the coronary arteries of the patients became totally occluded. Implant patency with collateralization was 80%; some patients were living entirely on blood supplied by mammary implants. Operative mortality was 2%. There was improvement in anginal pain in 85% of the cases and in left ventricular failure, in 67%. Studies of 43 patients with single implants, followed for up to 20 years, showed that 54% survived for an average of eight years and 46% have survived for an average of 12 years, and are still living. Double-ventricular implants in 42 patients with triple coronary artery disease showed that 90.5% survived for an average of five years, compared with 53% for medically treated patients. The technique of mammary implants is described and the procedure is compared to aortocoronary bypass grafts.", "contents": "Revascularization via healthy myocardial arteriolar networks compared with that through diseased surface coronary arteries. Myocardial mammary artery implants revascularize via the undiseased coronary microcirculation. Such implants, followed for up to 20 years, showed no atherosclerosis, while the coronary arteries of the patients became totally occluded. Implant patency with collateralization was 80%; some patients were living entirely on blood supplied by mammary implants. Operative mortality was 2%. There was improvement in anginal pain in 85% of the cases and in left ventricular failure, in 67%. Studies of 43 patients with single implants, followed for up to 20 years, showed that 54% survived for an average of eight years and 46% have survived for an average of 12 years, and are still living. Double-ventricular implants in 42 patients with triple coronary artery disease showed that 90.5% survived for an average of five years, compared with 53% for medically treated patients. The technique of mammary implants is described and the procedure is compared to aortocoronary bypass grafts."}
{"id": "PMID:1078672", "title": "A scanning electron microscopic study of the morphology and geometry of neural surfaces and structures associated with the vestibular apparatus of the pigeon.", "content": "The scanning electron microscope (SEM) was used to investigate the morphology of the neuroepithelial regions of the vestibular ampullary structures in 47 White King pigeons. The specific neural surfaces studied were (1) the cristae ampullares of the vertical and lateral membranous ampullae, (2) the hair cells lining the cristae, (3) the ampullary nerve fibers, and (4) the bipolar cells of the vestibular (Scarpa's) ganglion. Additionally, some observations of the gross anatomical structures of the bony labyrinth are given. Arguments are advanced which show that if the surface area of a given semicircular canal can be projected onto one of the three normal head planes, then that canal can be made to respond to motion in the appropriate plane, provided that the projected area is sufficiently large to achieve a threshold pressure as determined by a generalized form of Groen's equation ('57). With regard to the cristae ampullares, it is hypothesized that their surface areas can be described by means of a revolved catenary, i.e., a catenoid of revolution. (The catenary is found in nature as the approximate shape taken by a flexible cable when it is suspended at two points). The surface area of a catenoid provides a minimum surface of revolution. In the context of a crista, this implies that the given number of hair cells could not be fitted onto a smaller surface area. One advantage of this is that nature is able to utilize a thinner cupula than would be possible with other configurations and therefore an increased sensitivity to cupular motion can be realized. A second important factor is that all hair cells must revolve (by way of cupular motion) about the same centre of rotation in response to angular acceleration. Thus, all of the orthogonally-positioned hair cell tufts on the cristae surface may be stimulated simultaneously by way of a tangential shear. Other arguments show that the classical \"swing door\" type of cupular motion is not consistent with SEM and other recent observations. Two alternate modes of cupular motion are presented, each of which requires far less energy expenditure than does the \"swing door\" cupula. The suggestion is then made that, during normal head movements, the cupula behaves as a drum much like the tympanic membrane and that only for large, non-physiological motions does the \"swinging door\" mode of cupular motion take place. It must be remembered, however, that cupular motions during normal physiological head movements are infinitesimally small (Oman and Young, '72).", "contents": "A scanning electron microscopic study of the morphology and geometry of neural surfaces and structures associated with the vestibular apparatus of the pigeon. The scanning electron microscope (SEM) was used to investigate the morphology of the neuroepithelial regions of the vestibular ampullary structures in 47 White King pigeons. The specific neural surfaces studied were (1) the cristae ampullares of the vertical and lateral membranous ampullae, (2) the hair cells lining the cristae, (3) the ampullary nerve fibers, and (4) the bipolar cells of the vestibular (Scarpa's) ganglion. Additionally, some observations of the gross anatomical structures of the bony labyrinth are given. Arguments are advanced which show that if the surface area of a given semicircular canal can be projected onto one of the three normal head planes, then that canal can be made to respond to motion in the appropriate plane, provided that the projected area is sufficiently large to achieve a threshold pressure as determined by a generalized form of Groen's equation ('57). With regard to the cristae ampullares, it is hypothesized that their surface areas can be described by means of a revolved catenary, i.e., a catenoid of revolution. (The catenary is found in nature as the approximate shape taken by a flexible cable when it is suspended at two points). The surface area of a catenoid provides a minimum surface of revolution. In the context of a crista, this implies that the given number of hair cells could not be fitted onto a smaller surface area. One advantage of this is that nature is able to utilize a thinner cupula than would be possible with other configurations and therefore an increased sensitivity to cupular motion can be realized. A second important factor is that all hair cells must revolve (by way of cupular motion) about the same centre of rotation in response to angular acceleration. Thus, all of the orthogonally-positioned hair cell tufts on the cristae surface may be stimulated simultaneously by way of a tangential shear. Other arguments show that the classical \"swing door\" type of cupular motion is not consistent with SEM and other recent observations. Two alternate modes of cupular motion are presented, each of which requires far less energy expenditure than does the \"swing door\" cupula. The suggestion is then made that, during normal head movements, the cupula behaves as a drum much like the tympanic membrane and that only for large, non-physiological motions does the \"swinging door\" mode of cupular motion take place. It must be remembered, however, that cupular motions during normal physiological head movements are infinitesimally small (Oman and Young, '72)."}
{"id": "PMID:1078673", "title": "Idiopathic thrombocytopenic purpura: report of case.", "content": "A patient with classic symptoms of blood dyscrasia, specifically idiopathic thrombocytopenic purpura, failed to receive laboratory tests because the condition had gone undetected by a dentist and a physician. However, after the patient was given a provisional diagnosis supported by laboratory test results, one of the general treatment procedures for idiopathic thrombocytopenic purpura that consisted of a regimen of corticosteroids was instituted and was proved successful. More radical procedures, such as transfusions of fresh whole blood or of fresh platelets, or a splenectomy, were unnecessary. The responsibility of a dentist to detect oral changes that are not of dental origin is emphasized.", "contents": "Idiopathic thrombocytopenic purpura: report of case. A patient with classic symptoms of blood dyscrasia, specifically idiopathic thrombocytopenic purpura, failed to receive laboratory tests because the condition had gone undetected by a dentist and a physician. However, after the patient was given a provisional diagnosis supported by laboratory test results, one of the general treatment procedures for idiopathic thrombocytopenic purpura that consisted of a regimen of corticosteroids was instituted and was proved successful. More radical procedures, such as transfusions of fresh whole blood or of fresh platelets, or a splenectomy, were unnecessary. The responsibility of a dentist to detect oral changes that are not of dental origin is emphasized."}
{"id": "PMID:1078674", "title": "Serious postextraction hemorrhage into the submandibular space: report of case.", "content": "A 47-year-old woman had a swelling in the floor of the mouth and in the submandibular area on the left side. About four hours earlier, a dentist had extracted the mandibular left second premolar and first molar. The rate and degree of the distension were alarming; the floor of the mouth was at a level with the incisal edges of the mandibular incisors. Five hours later, the floor of the mouth was elevated about 1 1/2 cm above the incisal edges of the teeth. Treatment of the patient and her unusual sensitivity to meperidine and promethazine that caused a depression into Cheyne-Stokes respiration and hypotension are described.", "contents": "Serious postextraction hemorrhage into the submandibular space: report of case. A 47-year-old woman had a swelling in the floor of the mouth and in the submandibular area on the left side. About four hours earlier, a dentist had extracted the mandibular left second premolar and first molar. The rate and degree of the distension were alarming; the floor of the mouth was at a level with the incisal edges of the mandibular incisors. Five hours later, the floor of the mouth was elevated about 1 1/2 cm above the incisal edges of the teeth. Treatment of the patient and her unusual sensitivity to meperidine and promethazine that caused a depression into Cheyne-Stokes respiration and hypotension are described."}
{"id": "PMID:1078675", "title": "Spontaneous and methylcholanthrene-enhanced thyroiditis in BUF rats. I. The incidence and severity of the disease, and the genetics of susceptibility.", "content": "Virgin female Buffalo strain (BUF) rats that were treated with methylcholanthrene (MC) had a higher incidence of lymphocytic thyroiditis than untreated BUF rats. The degree of infiltration was also greater in MC-treated rats. The incidence of thyroiditis in retired breeder female BUF rats approached that found in MC-treated rats. The susceptibility to the effect of MC was not expressed in most F-1 hybrids of BUF and Lewis (LEW), a strain of rat that does not develop thyroiditis after treatment with MC.", "contents": "Spontaneous and methylcholanthrene-enhanced thyroiditis in BUF rats. I. The incidence and severity of the disease, and the genetics of susceptibility. Virgin female Buffalo strain (BUF) rats that were treated with methylcholanthrene (MC) had a higher incidence of lymphocytic thyroiditis than untreated BUF rats. The degree of infiltration was also greater in MC-treated rats. The incidence of thyroiditis in retired breeder female BUF rats approached that found in MC-treated rats. The susceptibility to the effect of MC was not expressed in most F-1 hybrids of BUF and Lewis (LEW), a strain of rat that does not develop thyroiditis after treatment with MC."}
{"id": "PMID:1078676", "title": "Relation of DNA synthesis and suppression to development of memory by T cells.", "content": "Parental thymocytes, inoculated into lethally irradiated F-1 mice, synthesize DNA in response to the histocompatibility antigens of the reciprocal parent in the F-1 cross. The DNA synthetic response is characterized by 1 to 3 day latent period and a 1-to-2-day period of peak activity which is followed by a sharp decline. Cells transferred to secondary recipients at the time of peak activity in the primary hosts respond only minimally in the spleens of the secondary hosts. Cells transferred 1 day later, when DNA synthetic activity in the primary hosts is sharply curtailed, exhibit striking secondary responsiveness. Thus, the decline in activity in the primary host is not due to the loss of potentially reactive cells from the spleens. It seems more likely that the preceding response creates a suppressive milieu in the primary host. The inability to transfer significant secondary responsiveness with cells harvested at the time of peak activity in primary hosts suggests that the acquistion of memory by these cells requires more than their colonal expansion. The occurrence of an additional differentiational event is suggested by the correlation of a marked increase in memory with the sharp cessation of the primary DNA synthetic response.", "contents": "Relation of DNA synthesis and suppression to development of memory by T cells. Parental thymocytes, inoculated into lethally irradiated F-1 mice, synthesize DNA in response to the histocompatibility antigens of the reciprocal parent in the F-1 cross. The DNA synthetic response is characterized by 1 to 3 day latent period and a 1-to-2-day period of peak activity which is followed by a sharp decline. Cells transferred to secondary recipients at the time of peak activity in the primary hosts respond only minimally in the spleens of the secondary hosts. Cells transferred 1 day later, when DNA synthetic activity in the primary hosts is sharply curtailed, exhibit striking secondary responsiveness. Thus, the decline in activity in the primary host is not due to the loss of potentially reactive cells from the spleens. It seems more likely that the preceding response creates a suppressive milieu in the primary host. The inability to transfer significant secondary responsiveness with cells harvested at the time of peak activity in primary hosts suggests that the acquistion of memory by these cells requires more than their colonal expansion. The occurrence of an additional differentiational event is suggested by the correlation of a marked increase in memory with the sharp cessation of the primary DNA synthetic response."}
{"id": "PMID:1078677", "title": "Capacity of sensitized thymus-derived lymphocytes to induce fatal lymphocytic choriomeningitis is restricted by the H-2 gene complex.", "content": "Adoptive immunization of syngeneic, immunosuppressed recipients infected with lymphocytic choriomeningitis (LCM) virus causes fatal neurologic disease within 2 to 4 days of cell transfer, providing that donors are sampled when the in vitro 51-Cr release assay shows maximal specific activity of sensitized thymus-derived lymphocytes (T cells). Prior treatment of immune spleen cells with AKR anti-omicron ascitic fluid and complement causes total abrogation of this in vivo activity. Fatal neurologic disease is induced only when donor and recipient share at least one set of H-2 antigenic specificities. Parent yields F1 and F1 yields parent combinations are as effective as syngeneic systems, but mice given allogeneic immune cells survive as long as controlsmdifferences at the M-locus in H-2 compatible mice do not inhibit effector activity. Homing of transferred lymphocytes to spleen is similar in syngeneic or allogeneic recipients, but only syngeneic immune cells cross the blood-cerebrospinal fluid (CSF) barrier and cause choriomeningitis. Fatal LCM, is, therefore, apparently induced by a specifically sensitized omicron-bearing cell population, activity of which is restricted by the H-2 gene complex.", "contents": "Capacity of sensitized thymus-derived lymphocytes to induce fatal lymphocytic choriomeningitis is restricted by the H-2 gene complex. Adoptive immunization of syngeneic, immunosuppressed recipients infected with lymphocytic choriomeningitis (LCM) virus causes fatal neurologic disease within 2 to 4 days of cell transfer, providing that donors are sampled when the in vitro 51-Cr release assay shows maximal specific activity of sensitized thymus-derived lymphocytes (T cells). Prior treatment of immune spleen cells with AKR anti-omicron ascitic fluid and complement causes total abrogation of this in vivo activity. Fatal neurologic disease is induced only when donor and recipient share at least one set of H-2 antigenic specificities. Parent yields F1 and F1 yields parent combinations are as effective as syngeneic systems, but mice given allogeneic immune cells survive as long as controlsmdifferences at the M-locus in H-2 compatible mice do not inhibit effector activity. Homing of transferred lymphocytes to spleen is similar in syngeneic or allogeneic recipients, but only syngeneic immune cells cross the blood-cerebrospinal fluid (CSF) barrier and cause choriomeningitis. Fatal LCM, is, therefore, apparently induced by a specifically sensitized omicron-bearing cell population, activity of which is restricted by the H-2 gene complex."}
{"id": "PMID:1078678", "title": "The selective action of poly A:U on the electrophoretic mobility and surface charge of a subpopulation of B cells.", "content": "Electrophoretic mobility (E.P.M.) of lymphoid cells has been determined in three species. In chicks, the E.P.M. of bursa cells was 0.47 plus or minus 0.07 and of thymocytes 0.64 plus or minus 0.10 mum/sec/volt/cm. On the basis of the E.P.M. profile of the Ficoll gradient purified lymphocytes from the rat spleen, two populations of cells present in the proportion of 65 and 35% were identifiable. The former had a mobility of 1.06 plus or minus 0.08 and the latter 1.24 plus or minus 0.09. The E.P.M. of lymphocytes prepared from athymic nude mice was 0.86 plus or minus 0.06 with a Gaussian distribution profile. On incubation of these cells with poly adenylate-poly uridylate (poly A:U) for 30 min at 38 degrees C followed by extensive washings, the E.P.M. of thymocytes from the chick and rat as well as the thymus-derived cells in rat spleen remained essentially unchanged. On the other hand, poly A:U increased the E.P.M. of avian bursa cells and of a subpopulation of B cells in rat spleen and of splenocytes from nude mice. The proportion of cells markedly affected by poly A:U was 70 to 83% of the total B cells in these species.", "contents": "The selective action of poly A:U on the electrophoretic mobility and surface charge of a subpopulation of B cells. Electrophoretic mobility (E.P.M.) of lymphoid cells has been determined in three species. In chicks, the E.P.M. of bursa cells was 0.47 plus or minus 0.07 and of thymocytes 0.64 plus or minus 0.10 mum/sec/volt/cm. On the basis of the E.P.M. profile of the Ficoll gradient purified lymphocytes from the rat spleen, two populations of cells present in the proportion of 65 and 35% were identifiable. The former had a mobility of 1.06 plus or minus 0.08 and the latter 1.24 plus or minus 0.09. The E.P.M. of lymphocytes prepared from athymic nude mice was 0.86 plus or minus 0.06 with a Gaussian distribution profile. On incubation of these cells with poly adenylate-poly uridylate (poly A:U) for 30 min at 38 degrees C followed by extensive washings, the E.P.M. of thymocytes from the chick and rat as well as the thymus-derived cells in rat spleen remained essentially unchanged. On the other hand, poly A:U increased the E.P.M. of avian bursa cells and of a subpopulation of B cells in rat spleen and of splenocytes from nude mice. The proportion of cells markedly affected by poly A:U was 70 to 83% of the total B cells in these species."}
{"id": "PMID:1078679", "title": "Relationship of cloning inhibition factor, \"lymphotoxin\" factor, and proliferation inhibition factor release in vitro by mitogen-activated human lymphoctes.", "content": "Human lymphocytes were stimulated in vitro with mitogens, phytohemagglutinin and concanavalin A to secrete proliferation inhibitory factor (PIF), cloning inhibitory factor (CIF), and lymphotoxin (LT). These three activities were demonstrable in the same supernatant, moreover, the particular effect observed was shown to depend on the concentration of the medium and the type of target cell employed. In general, the medium effects on target cells were: a) cytotoxic at high concentrations, b) growth inhibitory at intermediate concentrations, and c) only temporarily growth inhibitory at low concentrations. The absolute concentration producing a certain effect varied, depending on the target cell type employed. In addition, the sensitivity of the target cells to LT parallels the sensitivity of the cell to each of the other activities, PIF and CIF, and no species specificity was observed.", "contents": "Relationship of cloning inhibition factor, \"lymphotoxin\" factor, and proliferation inhibition factor release in vitro by mitogen-activated human lymphoctes. Human lymphocytes were stimulated in vitro with mitogens, phytohemagglutinin and concanavalin A to secrete proliferation inhibitory factor (PIF), cloning inhibitory factor (CIF), and lymphotoxin (LT). These three activities were demonstrable in the same supernatant, moreover, the particular effect observed was shown to depend on the concentration of the medium and the type of target cell employed. In general, the medium effects on target cells were: a) cytotoxic at high concentrations, b) growth inhibitory at intermediate concentrations, and c) only temporarily growth inhibitory at low concentrations. The absolute concentration producing a certain effect varied, depending on the target cell type employed. In addition, the sensitivity of the target cells to LT parallels the sensitivity of the cell to each of the other activities, PIF and CIF, and no species specificity was observed."}
{"id": "PMID:1078680", "title": "Anti-Ig-triggered movements of lymphocytes-specificity and lack of evidence for directional migration.", "content": "Interaction of anti-immunoglobulin antibodies with the surface Ig of B lymphocytes leads to capping and endocytosis of the immune complexes and to a brief stimulation of translational motility. Lymphocytes are not stimulated to move by interaction with other ligands, such as antibodies to the theta isoantigen, to H2 determinants, or to determinants recognized by heterologous anti-lymphocyte antibodies. Furthermore, anti-lymphocyte antibodies block the motility produced by anti-Ig. This effect was not seen in cells treated with colchicine, suggesting that the anti-lymphocyte effect may be associated with hyperstabilization of microtubules. Finally, we report on preliminary experiments using chemotactic chambers which show that anti-Ig stimulates random but not directional movement.", "contents": "Anti-Ig-triggered movements of lymphocytes-specificity and lack of evidence for directional migration. Interaction of anti-immunoglobulin antibodies with the surface Ig of B lymphocytes leads to capping and endocytosis of the immune complexes and to a brief stimulation of translational motility. Lymphocytes are not stimulated to move by interaction with other ligands, such as antibodies to the theta isoantigen, to H2 determinants, or to determinants recognized by heterologous anti-lymphocyte antibodies. Furthermore, anti-lymphocyte antibodies block the motility produced by anti-Ig. This effect was not seen in cells treated with colchicine, suggesting that the anti-lymphocyte effect may be associated with hyperstabilization of microtubules. Finally, we report on preliminary experiments using chemotactic chambers which show that anti-Ig stimulates random but not directional movement."}
{"id": "PMID:1078681", "title": "Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. I. Population studies.", "content": "The Ly-4 locus defines an alloantigenic specificity present on the surface of lymphocytes of some mouse strains and detected in lymphocytotoxicity tests by an antiserum (BALB/c times SWR)F1 anti-B10.D2. In this paper are presented results which indicate that Ly-4.2 is predominantly represented on B cells and not T cells. In cytotoxicity tests with rabbit complement, the distribution of Ly-4.2 is restricted: thymus 5%, TDL 12%, spleen 60%, lymph node 30%, bone marrow 36%, Peyer's patches 55%, peritoneal exudate cells 35%, and peripheral lymphocytes 35%. This distribution is the reciprocal of that obtained with anti-Thy-1 antiserum. Further, when both reagents were used together, almost all cells in these tissues were lysed, excepting those with bone marrow. When anti-Ly-4.2 and anti-Thy-1.2 were used sequentially, it was apparent that these specificities were present on different cell populations. As Thy-1 is a marker for T cells, Ly-4 is therefore, presumably, a marker for B cells. This conclusion was confirmed by testing mice depleted of T cells (\"nude\"; thymectomy + anti-lymphocyte serum treatment; thymectomy, irradiation + reconstitution with bone marrow) where the majority of lymphocytes were Ly-4.2+, Thy-1.2-; and by testing tumors, where, for the most part, this reciprocal relationship held true. Appropriate studies indicate that the Ly-4.2 antibody does not detect a surface immunoglobulin allotype. Anti-Ly-4.2 anti-serum may provide a useful alloantigenic marker distinct from immunoglobulin, mouse-specific bone marrow-derived lymphocyte anti;en, and \"beta\" for detecting some or all, B cells.", "contents": "Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. I. Population studies. The Ly-4 locus defines an alloantigenic specificity present on the surface of lymphocytes of some mouse strains and detected in lymphocytotoxicity tests by an antiserum (BALB/c times SWR)F1 anti-B10.D2. In this paper are presented results which indicate that Ly-4.2 is predominantly represented on B cells and not T cells. In cytotoxicity tests with rabbit complement, the distribution of Ly-4.2 is restricted: thymus 5%, TDL 12%, spleen 60%, lymph node 30%, bone marrow 36%, Peyer's patches 55%, peritoneal exudate cells 35%, and peripheral lymphocytes 35%. This distribution is the reciprocal of that obtained with anti-Thy-1 antiserum. Further, when both reagents were used together, almost all cells in these tissues were lysed, excepting those with bone marrow. When anti-Ly-4.2 and anti-Thy-1.2 were used sequentially, it was apparent that these specificities were present on different cell populations. As Thy-1 is a marker for T cells, Ly-4 is therefore, presumably, a marker for B cells. This conclusion was confirmed by testing mice depleted of T cells (\"nude\"; thymectomy + anti-lymphocyte serum treatment; thymectomy, irradiation + reconstitution with bone marrow) where the majority of lymphocytes were Ly-4.2+, Thy-1.2-; and by testing tumors, where, for the most part, this reciprocal relationship held true. Appropriate studies indicate that the Ly-4.2 antibody does not detect a surface immunoglobulin allotype. Anti-Ly-4.2 anti-serum may provide a useful alloantigenic marker distinct from immunoglobulin, mouse-specific bone marrow-derived lymphocyte anti;en, and \"beta\" for detecting some or all, B cells."}
{"id": "PMID:1078682", "title": "Rabbit lymphocyte populations responding to haptenic and carrier determinants for DNA synthesis.", "content": "Mesenteric lymph node cells from rabbits primed with dinitrophenyl derivative of either Ascaris extract (DNP-Asc) or ragweed fraction D (DNP-Rag) were stimulated by the priming antigen, free homologous carrier, or with the hapten conjugated with rabbit serum albumin (DNP-RSA), and increase in DNA synthesis was observed by 3H-thymidine incorporation. The results showed that both free carrier and DNP-RSA stimulated DNA synthesis. The response of the primed lymph node cells to DNP-homologous conjugate was slightly higher than that to free carrier, but the optimal concentration of both antigens for maximal thymidine incorporation was 10 to 100 mug/ml. This concentration was about 100 times higher than the optimal concentration of the same antigens for maximal antibody response in vitro. The DNA synthetic response to DNP-RSA was much less than that obtained by free carrier, and the optimal concentration of DNP-RSA for the response was comparable to the concentration of DNP-homologous carrier conjugate to induce maximum anti-DNP antibody response. The relative importance of immunoglobulin bearing (B) cells and T cells in the DNA synthetic responses was assessed by fractionating lymph node cells with antigen-coated or anti-immunoglobulin coated  cellular immunosorbent. The results indicated that hapten-specific, immunoglobulin-bearing cells (B cells) are responsible for the DNA-synthetic response to DNP-RSA, whereas B cells play a minimal role in the response to free carrier.", "contents": "Rabbit lymphocyte populations responding to haptenic and carrier determinants for DNA synthesis. Mesenteric lymph node cells from rabbits primed with dinitrophenyl derivative of either Ascaris extract (DNP-Asc) or ragweed fraction D (DNP-Rag) were stimulated by the priming antigen, free homologous carrier, or with the hapten conjugated with rabbit serum albumin (DNP-RSA), and increase in DNA synthesis was observed by 3H-thymidine incorporation. The results showed that both free carrier and DNP-RSA stimulated DNA synthesis. The response of the primed lymph node cells to DNP-homologous conjugate was slightly higher than that to free carrier, but the optimal concentration of both antigens for maximal thymidine incorporation was 10 to 100 mug/ml. This concentration was about 100 times higher than the optimal concentration of the same antigens for maximal antibody response in vitro. The DNA synthetic response to DNP-RSA was much less than that obtained by free carrier, and the optimal concentration of DNP-RSA for the response was comparable to the concentration of DNP-homologous carrier conjugate to induce maximum anti-DNP antibody response. The relative importance of immunoglobulin bearing (B) cells and T cells in the DNA synthetic responses was assessed by fractionating lymph node cells with antigen-coated or anti-immunoglobulin coated  cellular immunosorbent. The results indicated that hapten-specific, immunoglobulin-bearing cells (B cells) are responsible for the DNA-synthetic response to DNP-RSA, whereas B cells play a minimal role in the response to free carrier."}
{"id": "PMID:1078696", "title": "Competition between concanavalin A-induced stimulatory and inhibitory effects in the in vitro immune response to antigen.", "content": "The humoral response of nude spleen cells (b cells) to sheep erythrocytes was measured in the presence of varying numbers of concanavalin A (ConA)-acvated stimulatory spleen T cells (helper) and Con A-activated inhibitory spleen T cells (suppressor) from BDF1 mice. It was found that suppressive effects could be reversed by the presence of additional numbers of stimulatory cells. These results seem incompatible with the hypothesis that suppression is mediated by supraoptimal numbers of stimulatory cells and provides additional evidence that separate populations of T cells mediate stimulation and suppression.", "contents": "Competition between concanavalin A-induced stimulatory and inhibitory effects in the in vitro immune response to antigen. The humoral response of nude spleen cells (b cells) to sheep erythrocytes was measured in the presence of varying numbers of concanavalin A (ConA)-acvated stimulatory spleen T cells (helper) and Con A-activated inhibitory spleen T cells (suppressor) from BDF1 mice. It was found that suppressive effects could be reversed by the presence of additional numbers of stimulatory cells. These results seem incompatible with the hypothesis that suppression is mediated by supraoptimal numbers of stimulatory cells and provides additional evidence that separate populations of T cells mediate stimulation and suppression."}
{"id": "PMID:1078697", "title": "Corneal thickness measured by interferometry.", "content": "An optical method for measuring the thickness of transparent structures has been developed, and has been used to measure, in vivo, the thickness of the human cornea. The thickness is measured by placing the anterior surface of the cornea at the focus of a beam of coherent laser light and then measuring the spacing between the interference fringes generated by the reflected light. The thickness is then calculated from the fringe spacing. The method has been used to measure corneal thickness in frog and human corneas. These measurements have been correlated with histologic and pachometer measurements of corneal thickness. A significant capability of this technique is to measure the thickness of optically opaque corneas.", "contents": "Corneal thickness measured by interferometry. An optical method for measuring the thickness of transparent structures has been developed, and has been used to measure, in vivo, the thickness of the human cornea. The thickness is measured by placing the anterior surface of the cornea at the focus of a beam of coherent laser light and then measuring the spacing between the interference fringes generated by the reflected light. The thickness is then calculated from the fringe spacing. The method has been used to measure corneal thickness in frog and human corneas. These measurements have been correlated with histologic and pachometer measurements of corneal thickness. A significant capability of this technique is to measure the thickness of optically opaque corneas."}
{"id": "PMID:1078699", "title": "Runt syndrome induced in the rat by polyinosinic-polycytidylic acid.", "content": "Polyinosinic with polycytidylic acid (poly l with poly C), a double-stranded synthetic RNA, produced in newborn rats a runt syndrome characterized by mortality and retarded growth rates of the total body, thymus, and kidneys. In contrast, it induced a hyperplasia in the epidermis and in the spleen. Within 10 days of treatment, the epidermis became 2 or 3 times thicker and the spleen mass was increased by 50%. The epidermal hyperplasia involved all layers, but hair follicles were excluded. Splenic hyperplasia did not result from accelerated erythropoiesis. Double-stranded RNA was required; single-stranded homopolymers were ineffective. Theophylline, a phosphodiesterase inhibitor, did not potentiate the effects. The uptake of iododeoxyuridine-125 was not enhanced in the hyperplastic epidermis or spleen. Thus we concluded that poly l with poly C can retard the growth of some organs in newborn rats, but that it causes epidermis and spleen to accumulate cells. The cytokinetic mechanisms involved in these contrasting effects were not clear.", "contents": "Runt syndrome induced in the rat by polyinosinic-polycytidylic acid. Polyinosinic with polycytidylic acid (poly l with poly C), a double-stranded synthetic RNA, produced in newborn rats a runt syndrome characterized by mortality and retarded growth rates of the total body, thymus, and kidneys. In contrast, it induced a hyperplasia in the epidermis and in the spleen. Within 10 days of treatment, the epidermis became 2 or 3 times thicker and the spleen mass was increased by 50%. The epidermal hyperplasia involved all layers, but hair follicles were excluded. Splenic hyperplasia did not result from accelerated erythropoiesis. Double-stranded RNA was required; single-stranded homopolymers were ineffective. Theophylline, a phosphodiesterase inhibitor, did not potentiate the effects. The uptake of iododeoxyuridine-125 was not enhanced in the hyperplastic epidermis or spleen. Thus we concluded that poly l with poly C can retard the growth of some organs in newborn rats, but that it causes epidermis and spleen to accumulate cells. The cytokinetic mechanisms involved in these contrasting effects were not clear."}
{"id": "PMID:1078700", "title": "Tumor induction by Moloney sarcoma virus in athymic nude mice.", "content": "Athymic nude (nu/nu) mice, normal NMRI (plus/plus) mice, and nu/plus heterozygotes, inoculated at birth with the Moloney strain of murine sarcoma virus (M-MSV), did not differ either in tumor growth rate or incidence or in mortality due to tumor development; neither did nude (nu/nu) and normal NMRI mice inoculated with M-MSV at 30 days of age differ in the initial tumor growth rate. However, tumors induced in adult nude mice grew progressively and eventually killed the hosts, whereas tumors induced in adult normal NMRI mice completely regressed. These results indicated that thymus-dependent immunity, though not active in M-MSV tumor induction, determined whether the tumor, once formed, would regress or kill the host.", "contents": "Tumor induction by Moloney sarcoma virus in athymic nude mice. Athymic nude (nu/nu) mice, normal NMRI (plus/plus) mice, and nu/plus heterozygotes, inoculated at birth with the Moloney strain of murine sarcoma virus (M-MSV), did not differ either in tumor growth rate or incidence or in mortality due to tumor development; neither did nude (nu/nu) and normal NMRI mice inoculated with M-MSV at 30 days of age differ in the initial tumor growth rate. However, tumors induced in adult nude mice grew progressively and eventually killed the hosts, whereas tumors induced in adult normal NMRI mice completely regressed. These results indicated that thymus-dependent immunity, though not active in M-MSV tumor induction, determined whether the tumor, once formed, would regress or kill the host."}
{"id": "PMID:1078701", "title": "Laser-induced hemostasis in the canine stomach. Use of a flexible fiberoptic delivery system.", "content": "A flexible fiberoptic system has been used to deliver visible laser light into the upper gastrointestinal tract to arrest bleeding from various induced lesions. This procedure has achieved hemostasis at bleeding sites in the gastrointestinal tract of animals and suggests that bleeding lesions in the heman may be treated similarly without resort to surgical intervention or intensive medical management.", "contents": "Laser-induced hemostasis in the canine stomach. Use of a flexible fiberoptic delivery system. A flexible fiberoptic system has been used to deliver visible laser light into the upper gastrointestinal tract to arrest bleeding from various induced lesions. This procedure has achieved hemostasis at bleeding sites in the gastrointestinal tract of animals and suggests that bleeding lesions in the heman may be treated similarly without resort to surgical intervention or intensive medical management."}
{"id": "PMID:1078702", "title": "Antitrypsin phenotypes in St. Louis.", "content": "Antitrypsin phenotypes were determined from 2,285 donors at the Barnes Hospital Blood Bank in St. Louis . Results resembled those obtained from Central Europeans: there were fewer proteinase inhibitor (Pi) Z alleles in St. Louis than in Scandinavian test groups. Interestingly, major antifrypsin variants occurred only 40% as frequently in black as in the balance of the study group.", "contents": "Antitrypsin phenotypes in St. Louis. Antitrypsin phenotypes were determined from 2,285 donors at the Barnes Hospital Blood Bank in St. Louis . Results resembled those obtained from Central Europeans: there were fewer proteinase inhibitor (Pi) Z alleles in St. Louis than in Scandinavian test groups. Interestingly, major antifrypsin variants occurred only 40% as frequently in black as in the balance of the study group."}
{"id": "PMID:1078707", "title": "Open-heart surgery in von Willebrand's disease.", "content": "The case is presented of a hemophilia carrier, also affected by von Willebrand's disease, who underwent aortic valve replacement. The clinical and laboratory findings of von Willebrand's disease (prolonged bleeding time, low factor VIII, and abnormal platelet activity) are discussed, and a protocol for management of patients with low factor VIII levels (such as hemophilia carriers and subjects with hemophilia A or von Willebrand's disease) undergoing open-heart surgery is proposed. Our case proves that corrective cardiac surgery in similar circumstances is feasible so long as adequate levels of factor VIII are maintained.", "contents": "Open-heart surgery in von Willebrand's disease. The case is presented of a hemophilia carrier, also affected by von Willebrand's disease, who underwent aortic valve replacement. The clinical and laboratory findings of von Willebrand's disease (prolonged bleeding time, low factor VIII, and abnormal platelet activity) are discussed, and a protocol for management of patients with low factor VIII levels (such as hemophilia carriers and subjects with hemophilia A or von Willebrand's disease) undergoing open-heart surgery is proposed. Our case proves that corrective cardiac surgery in similar circumstances is feasible so long as adequate levels of factor VIII are maintained."}
{"id": "PMID:1078705", "title": "[Effect of acceleration on the sugar content of rabbit cerebrospinal fluid].", "content": "The sugar content in the liquor of rabbits exposed to accelerations ranging from several minutes to several hours was studied. The animals were accelerated at 3 g in a centrifuge with a radius of 55 cm and a velocity of 70 rpm, in the Khilov's swing and in a rotating device simulating angular accelerations. The liquor was sampled by the suboccipital puncture and the sugar content was measured according to Hagedorn-Jensen. A statistically significant increase in the sugar content was noted after every exposure to accelerations of alternating signs than with accelerations of a constant value. The experiments on labyrinth-ectomized animals showed a certain effect of the vestibular analyzer on the carbohydrate metabolism. However, when the equilibrium function reached compensation, the sugar content in the liquor returned to the pretest level. Therefore, this reaction involves not only the vestibular system but also the optic, proprio- and interoceptive afferent systems. A comparison of the intracysternal and intramuscular injections of epinephrine points to the central (in contrast to the hematogenic) nature of an increase in the sugar content during the first minutes of acceleration. The electric stimulation of the anterior and posterior hypothalamus by means of implanted electrodes produced the same elevation of the liquor sugar as accelerations. It can be postulated that afferent impulses from the analyzer systems reach the hypothalamic centers, thus exciting the autonomic system and, first of all, the sympathic system.", "contents": "[Effect of acceleration on the sugar content of rabbit cerebrospinal fluid]. The sugar content in the liquor of rabbits exposed to accelerations ranging from several minutes to several hours was studied. The animals were accelerated at 3 g in a centrifuge with a radius of 55 cm and a velocity of 70 rpm, in the Khilov's swing and in a rotating device simulating angular accelerations. The liquor was sampled by the suboccipital puncture and the sugar content was measured according to Hagedorn-Jensen. A statistically significant increase in the sugar content was noted after every exposure to accelerations of alternating signs than with accelerations of a constant value. The experiments on labyrinth-ectomized animals showed a certain effect of the vestibular analyzer on the carbohydrate metabolism. However, when the equilibrium function reached compensation, the sugar content in the liquor returned to the pretest level. Therefore, this reaction involves not only the vestibular system but also the optic, proprio- and interoceptive afferent systems. A comparison of the intracysternal and intramuscular injections of epinephrine points to the central (in contrast to the hematogenic) nature of an increase in the sugar content during the first minutes of acceleration. The electric stimulation of the anterior and posterior hypothalamus by means of implanted electrodes produced the same elevation of the liquor sugar as accelerations. It can be postulated that afferent impulses from the analyzer systems reach the hypothalamic centers, thus exciting the autonomic system and, first of all, the sympathic system."}
{"id": "PMID:1078708", "title": "Results of direct coronary artery surgery in women.", "content": "In the past 4 years, among 260 patients receiving coronary bypass grafts for coronary artery disease and stable angina pectoris, there were 34 women (13 per cent). The operative mortality rate for women was 8 per cent (3 of 34), and one late death due to myocardial infarction occurred in spite of a patent coronary bypass graft. The intraoperative infarction rate was 20 per cent (6 per cent in men). Although preoperative cardiac pump and muscle function parameters were better in women than in men (p less than 0.05), postoperatively only 30 per cent of women showed improvement in function as compared with 50 per cent of men. At 6 to 46 months' follow-up, 84 per cent of women were free of angina in contrast with 94 per cent of men. The early (4 month) graft patency rate was 50 per cent (14 of 27 grafts), as opposed to 80 per cent (20 of 25 grafts) in men. These results indicate that, although coronary artery disease shows anatomic similarity in women and men, the result of coronary revascularization in women is inferior to that in the male population.", "contents": "Results of direct coronary artery surgery in women. In the past 4 years, among 260 patients receiving coronary bypass grafts for coronary artery disease and stable angina pectoris, there were 34 women (13 per cent). The operative mortality rate for women was 8 per cent (3 of 34), and one late death due to myocardial infarction occurred in spite of a patent coronary bypass graft. The intraoperative infarction rate was 20 per cent (6 per cent in men). Although preoperative cardiac pump and muscle function parameters were better in women than in men (p less than 0.05), postoperatively only 30 per cent of women showed improvement in function as compared with 50 per cent of men. At 6 to 46 months' follow-up, 84 per cent of women were free of angina in contrast with 94 per cent of men. The early (4 month) graft patency rate was 50 per cent (14 of 27 grafts), as opposed to 80 per cent (20 of 25 grafts) in men. These results indicate that, although coronary artery disease shows anatomic similarity in women and men, the result of coronary revascularization in women is inferior to that in the male population."}
{"id": "PMID:1078709", "title": "Survival and function after left ventricular aneurysmectomy.", "content": "Operative and late postoperative results in 55 patients undergoing resection of a discrete, saccular type left ventricular aneurysm are described. All patients had occlusive lesions of either the left anterior descending or left main coronary artery, and 72 per cent had significant multivessel disease. Preoperative factors that correlated significantly with decreased long-term survival were age greater than 60 years, severe congestive heart failure, and subnormal cardiac output at rest. The primary determinant of long-term postoperative survival appears to be the functional state of residual myocardium after aneurysmectomy. The survival rate for patients with multivessel disease was not less than that of patients with isolated lesions of the left anterior descending coronary artery. The impact of simultaneous aorto-coronary bypass grafting on postoperative results in patients with multivessel coronary disease is discussed. An advantage to concomitant revascularization with aneurysmectomy cannot be statistically corroborated in this series. However, improvement in results since the routine application of bypass grafting in appropriate cases, the uniform operative survival of bypassed patients with triple vessel disease, and the relatively high frequency of myocardial infarction as a cause of late postoperative death suggest that bypass grafting in patients with multivessel disease should be combined with aneurysmectomy to maximally improve long-term prognosis.", "contents": "Survival and function after left ventricular aneurysmectomy. Operative and late postoperative results in 55 patients undergoing resection of a discrete, saccular type left ventricular aneurysm are described. All patients had occlusive lesions of either the left anterior descending or left main coronary artery, and 72 per cent had significant multivessel disease. Preoperative factors that correlated significantly with decreased long-term survival were age greater than 60 years, severe congestive heart failure, and subnormal cardiac output at rest. The primary determinant of long-term postoperative survival appears to be the functional state of residual myocardium after aneurysmectomy. The survival rate for patients with multivessel disease was not less than that of patients with isolated lesions of the left anterior descending coronary artery. The impact of simultaneous aorto-coronary bypass grafting on postoperative results in patients with multivessel coronary disease is discussed. An advantage to concomitant revascularization with aneurysmectomy cannot be statistically corroborated in this series. However, improvement in results since the routine application of bypass grafting in appropriate cases, the uniform operative survival of bypassed patients with triple vessel disease, and the relatively high frequency of myocardial infarction as a cause of late postoperative death suggest that bypass grafting in patients with multivessel disease should be combined with aneurysmectomy to maximally improve long-term prognosis."}
{"id": "PMID:1078711", "title": "Surgical treatment for stable angina pectoris. Prospective randomized study.", "content": "To evaluate the role of coronary bypass treatment of stable angina, 72 patients were randomly divided into surgical and medical groups. They were followed with treadmill tests and repeat catheterization. Anginal symptoms decreased or disappeared in 89 per cent of those operated on and 65 per cent of those not operated on, but more of the former than of the latter became asymptomatic: 69 vs. 11 per cent respectively (p smaller than 0.01). Exercise tolerance time improved significantly (p smaller than 0.001) in both groups, but more in the group operated on, plus 78 per cent vs. plus 48 per cent, p smaller than 0.05. During a 28-month follow-up period, fewer cardiac complications occurred in the patients operated on--14 vs. 34 (p smaller than 0.02). Mortality was 9 per cent in the patients operated on and 14 per cent in those not operated on; this difference is not significant. The results indicate that improvement, though demonstrable in both groups, is greater with surgery, and quality of life during the first 28 months is better in patients who are operated on.", "contents": "Surgical treatment for stable angina pectoris. Prospective randomized study. To evaluate the role of coronary bypass treatment of stable angina, 72 patients were randomly divided into surgical and medical groups. They were followed with treadmill tests and repeat catheterization. Anginal symptoms decreased or disappeared in 89 per cent of those operated on and 65 per cent of those not operated on, but more of the former than of the latter became asymptomatic: 69 vs. 11 per cent respectively (p smaller than 0.01). Exercise tolerance time improved significantly (p smaller than 0.001) in both groups, but more in the group operated on, plus 78 per cent vs. plus 48 per cent, p smaller than 0.05. During a 28-month follow-up period, fewer cardiac complications occurred in the patients operated on--14 vs. 34 (p smaller than 0.02). Mortality was 9 per cent in the patients operated on and 14 per cent in those not operated on; this difference is not significant. The results indicate that improvement, though demonstrable in both groups, is greater with surgery, and quality of life during the first 28 months is better in patients who are operated on."}
{"id": "PMID:1078713", "title": "Clinical importance of lymphoblasts with T markers in childhood acute leukemia.", "content": "Of 48 children with acute lymphocytic leukemia 11 had blast cells with receptors for sheep erythrocytes in their initial bone-marrow aspirates and 37 did not. A comparison of selected clinical features indicated striking differences between the two groups. Leukemia with the receptors was associated with a high proportion of older children, predominantly boys, a thymic mass, and a high white-cell count at diagnosis. In contrast, the 37 children with leukemia without the receptors were generally less than five years of age, with a nearly equal distribution of boys and girls; all but one had normal chest roentgenograms, and only one had a white-cell count greater than 100,000. Thus, the presence or absence of lymphoblasts with sheep erythrocyte receptors--a T-cell marker--distinguishes two forms of childhood acute lymphocytic leukemia, each with a distinct distribution of age and sex as well as other characteristic clinical features.", "contents": "Clinical importance of lymphoblasts with T markers in childhood acute leukemia. Of 48 children with acute lymphocytic leukemia 11 had blast cells with receptors for sheep erythrocytes in their initial bone-marrow aspirates and 37 did not. A comparison of selected clinical features indicated striking differences between the two groups. Leukemia with the receptors was associated with a high proportion of older children, predominantly boys, a thymic mass, and a high white-cell count at diagnosis. In contrast, the 37 children with leukemia without the receptors were generally less than five years of age, with a nearly equal distribution of boys and girls; all but one had normal chest roentgenograms, and only one had a white-cell count greater than 100,000. Thus, the presence or absence of lymphoblasts with sheep erythrocyte receptors--a T-cell marker--distinguishes two forms of childhood acute lymphocytic leukemia, each with a distinct distribution of age and sex as well as other characteristic clinical features."}
{"id": "PMID:1078715", "title": "Abnormal spontaneous rosette formation and rosette inhibition in lung carcinoma.", "content": "Fifty-five children with CID and known ADA status were studies at a workshop held in Albany, New York. Erythrocyte ADA determinations were performed in 22 of the 55 patients, 13 of whom were ADA negative. The ADA defect appears to be transmitted as an autosomal recessive trait. Some patients with CID and ADA deficiency have characteristic radiologic abnormalities of the skeleton, which are not found in other illnesses. The thymus glands of all patients with CID and ADA deficiency who could be examined have evidence of thymic involution manifested by presence of Hassall's corpuscles and differentiated germinal epithelium; this is in contrast to \"classic\" thymus findings in CID with normal ADA. Adenosine deaminase probably plays an important, although as yet undefined, role in lymphocyte development and/or function. The deficiency of ADA in CID is the first enzyme defect observed in a deficiency disease of specific immunity.", "contents": "Abnormal spontaneous rosette formation and rosette inhibition in lung carcinoma. Fifty-five children with CID and known ADA status were studies at a workshop held in Albany, New York. Erythrocyte ADA determinations were performed in 22 of the 55 patients, 13 of whom were ADA negative. The ADA defect appears to be transmitted as an autosomal recessive trait. Some patients with CID and ADA deficiency have characteristic radiologic abnormalities of the skeleton, which are not found in other illnesses. The thymus glands of all patients with CID and ADA deficiency who could be examined have evidence of thymic involution manifested by presence of Hassall's corpuscles and differentiated germinal epithelium; this is in contrast to \"classic\" thymus findings in CID with normal ADA. Adenosine deaminase probably plays an important, although as yet undefined, role in lymphocyte development and/or function. The deficiency of ADA in CID is the first enzyme defect observed in a deficiency disease of specific immunity."}
{"id": "PMID:1078720", "title": "Therapy of intention myoclonus with L-5-hydroxytryptophan and a peripheral decarboxylase inhibitor, MK 486.", "content": "Three patients with postanoxic intention myoclonus, two patients with intention tremor, and one patient with cerebral palsy were administered L-5-hydroxytryptophan (L-5HTP), the precursor of serotonin, in combination with MK 486, a peripheral amino acid decarboxylase inhibitor. L-5HTP combined with MK 486 were potent long-term therapeutic agents for postanoxic intention myoclonus, but had no effect on intention tremor or cerebral palsy. These drugs were well-tolerated by the patients, and more effective than any other known therapy for intention myoclonus. Cerebrospinal fluid concentration of 5-hydroxyindoleacetic acid, the main catabolite of serotonin, appeared low in two patients with intention myoclonus and increased markedly during drug therapy. Postanoxic intention myoclonus may be causally related to a deficiency of brain serotonin.", "contents": "Therapy of intention myoclonus with L-5-hydroxytryptophan and a peripheral decarboxylase inhibitor, MK 486. Three patients with postanoxic intention myoclonus, two patients with intention tremor, and one patient with cerebral palsy were administered L-5-hydroxytryptophan (L-5HTP), the precursor of serotonin, in combination with MK 486, a peripheral amino acid decarboxylase inhibitor. L-5HTP combined with MK 486 were potent long-term therapeutic agents for postanoxic intention myoclonus, but had no effect on intention tremor or cerebral palsy. These drugs were well-tolerated by the patients, and more effective than any other known therapy for intention myoclonus. Cerebrospinal fluid concentration of 5-hydroxyindoleacetic acid, the main catabolite of serotonin, appeared low in two patients with intention myoclonus and increased markedly during drug therapy. Postanoxic intention myoclonus may be causally related to a deficiency of brain serotonin."}
{"id": "PMID:1078721", "title": "Vestibulo-ocular function in patients with cerebellar atrophy.", "content": "Eye movement abnormalities were quantiatively assessed in four patients with clinically pure cerebellar atrophy (group A), six patients with brainstem plus cerebellar atrophy (group B), and five patients with Friedreich's ataxia (group C). Twelve patients had one or more types of spontaneous nystagmus; eight gaze nystagmus, three rebound nystagmus, wo positional nystagmus, and one vestibular nystagmus. Catoric-induced and rotatory-induced nystagmus was hyperactive in group A and diminished in group C. Group B had mixed responses. All patients demonstrated significant fixation instability and impaired smooth pursuit. There was dysmetria of voluntary saccades, with flutter and \"rebound\" saccades. Nine of 15 patients had significant slowing of induced saccades, including two patients in group A. It is concluded that quantitative vestibulocular tests can be useful in classifying the cerebellar atrophy syndromes.", "contents": "Vestibulo-ocular function in patients with cerebellar atrophy. Eye movement abnormalities were quantiatively assessed in four patients with clinically pure cerebellar atrophy (group A), six patients with brainstem plus cerebellar atrophy (group B), and five patients with Friedreich's ataxia (group C). Twelve patients had one or more types of spontaneous nystagmus; eight gaze nystagmus, three rebound nystagmus, wo positional nystagmus, and one vestibular nystagmus. Catoric-induced and rotatory-induced nystagmus was hyperactive in group A and diminished in group C. Group B had mixed responses. All patients demonstrated significant fixation instability and impaired smooth pursuit. There was dysmetria of voluntary saccades, with flutter and \"rebound\" saccades. Nine of 15 patients had significant slowing of induced saccades, including two patients in group A. It is concluded that quantitative vestibulocular tests can be useful in classifying the cerebellar atrophy syndromes."}
{"id": "PMID:1078722", "title": "[Immunological aspects of Hodgkin's disease].", "content": "Immunological test alterations in Hodgkin's disease are described. Cutaneous reactivity to various antigens (tuberculin, oidiomycin, trichophytin, parotitis virus, candida, etc.), cutaneous reactivity to sensitizing chemical agents (DNCB, DNFB), lymphocyte blastization in vitro with PHA and various antigens, the reaction of homologous lymphocytes transferred to the skin, and the stimulation by the Hodgkin lymphocytes of homologous lymphocytes in unidirectional mixed culture, are all impaired. Antibody immunity would appear to be normal. The impairment of the T system and normality of the B system would explain these patients' much greater susceptibility to viral than to bacterial diseases. On the basis of the most recent research, it is hypothesized that underlying these phenomena is a deficiency in the H-LA system which makes it unable to recognize certain neoplastic antigens (Reed-Strenberg reticular cells, for example); stimulation of the B system by the formation of anti-T antibodies and subsequent depletion of this system.", "contents": "[Immunological aspects of Hodgkin's disease]. Immunological test alterations in Hodgkin's disease are described. Cutaneous reactivity to various antigens (tuberculin, oidiomycin, trichophytin, parotitis virus, candida, etc.), cutaneous reactivity to sensitizing chemical agents (DNCB, DNFB), lymphocyte blastization in vitro with PHA and various antigens, the reaction of homologous lymphocytes transferred to the skin, and the stimulation by the Hodgkin lymphocytes of homologous lymphocytes in unidirectional mixed culture, are all impaired. Antibody immunity would appear to be normal. The impairment of the T system and normality of the B system would explain these patients' much greater susceptibility to viral than to bacterial diseases. On the basis of the most recent research, it is hypothesized that underlying these phenomena is a deficiency in the H-LA system which makes it unable to recognize certain neoplastic antigens (Reed-Strenberg reticular cells, for example); stimulation of the B system by the formation of anti-T antibodies and subsequent depletion of this system."}
{"id": "PMID:1078724", "title": "Differences in action potentials and accommodation of sensory and motor myelinated nerve fibres as computed on the basis of voltage clamp data.", "content": "1. Voltage clamp experiments were performed on sensory and motor nerve fibres of the frog using a digital computer for automatic experiment control and data recording. 2. Rates of rise and maximum amplitudes of potassium currents were determined in both sensory and motor fibres, so that comparative values of n infinity and tau-n could be obtained. 3. The results indicate that the n infinity-V curve for sensory fibres is displaced from the curve for motor fibres in a depolarising direction. The potassium kinetics are similar in both for voltage steps up to about - 20 m V, beyond which tau-n becomes progressively smaller for sensory than for motor fibres. 4. These comparative values of n affinity and tau-n have been used to calculate alpha-n and beta-n values for a model \"motor\" action potential by considering the Frankenhaeuser-Huxley computed action potential to be \"sensory\". This modification of the potassium system, together with some alteration to the sodium inactivation system produces a satisfactory model \"motor\" action potential. 5. The model sensory and motor action potentials behave quite similarly to their experimentally recorded counterparts with respect to action potential shape and relative duration, repetitive firing, accomodation and the simulated action of T.E.A.", "contents": "Differences in action potentials and accommodation of sensory and motor myelinated nerve fibres as computed on the basis of voltage clamp data. 1. Voltage clamp experiments were performed on sensory and motor nerve fibres of the frog using a digital computer for automatic experiment control and data recording. 2. Rates of rise and maximum amplitudes of potassium currents were determined in both sensory and motor fibres, so that comparative values of n infinity and tau-n could be obtained. 3. The results indicate that the n infinity-V curve for sensory fibres is displaced from the curve for motor fibres in a depolarising direction. The potassium kinetics are similar in both for voltage steps up to about - 20 m V, beyond which tau-n becomes progressively smaller for sensory than for motor fibres. 4. These comparative values of n affinity and tau-n have been used to calculate alpha-n and beta-n values for a model \"motor\" action potential by considering the Frankenhaeuser-Huxley computed action potential to be \"sensory\". This modification of the potassium system, together with some alteration to the sodium inactivation system produces a satisfactory model \"motor\" action potential. 5. The model sensory and motor action potentials behave quite similarly to their experimentally recorded counterparts with respect to action potential shape and relative duration, repetitive firing, accomodation and the simulated action of T.E.A."}
{"id": "PMID:1078725", "title": "Early receptor potential from the pineal photoreceptor.", "content": "Rapid photoresponses were obtained from the pineal organ of Rana catesbeiana and Rana esculenta comparable to the early receptor potential (ERP) of the retina of their lateral eyes. Light source was a xenon filled discharge tube with a maximum energy input of 365 J. The flash duration was 600 mu sec measured at 1/3 peak amplitude. The response pattern of the ERP of the exposed Epiphysis cerbri (intracranial portion of the frog's pineal organ) resembled the ERP of the isolated retina and the R1 deflection of the ERP of the eye-cup. It was greatly diminished during consecutive flashes and was absent during light adaptation. It was confined to the Epiphysis cerbri and was similar in time course and shap to, though smaller as, the ERP of the isolated retina. The photoresistant component of the ERP in the eye-cup was lacking in the Epiphysis cerbri and in the isolated retina. The action spectrum of the ERP of the Epiphysis cerbri closely resembled the photopigment absorption of rhodopsin indicating that the structural cones of the pineal organ contain a photopigment different from the retinal cones of the frog's lateral eye.", "contents": "Early receptor potential from the pineal photoreceptor. Rapid photoresponses were obtained from the pineal organ of Rana catesbeiana and Rana esculenta comparable to the early receptor potential (ERP) of the retina of their lateral eyes. Light source was a xenon filled discharge tube with a maximum energy input of 365 J. The flash duration was 600 mu sec measured at 1/3 peak amplitude. The response pattern of the ERP of the exposed Epiphysis cerbri (intracranial portion of the frog's pineal organ) resembled the ERP of the isolated retina and the R1 deflection of the ERP of the eye-cup. It was greatly diminished during consecutive flashes and was absent during light adaptation. It was confined to the Epiphysis cerbri and was similar in time course and shap to, though smaller as, the ERP of the isolated retina. The photoresistant component of the ERP in the eye-cup was lacking in the Epiphysis cerbri and in the isolated retina. The action spectrum of the ERP of the Epiphysis cerbri closely resembled the photopigment absorption of rhodopsin indicating that the structural cones of the pineal organ contain a photopigment different from the retinal cones of the frog's lateral eye."}
{"id": "PMID:1078726", "title": "[99mTc-polyphosphate scintigraphy of joints, xeroradiography and radiography of hands].", "content": "Joint imaging with 99m-Tc-Polyphosphate was carried out in 21 patients having inflammatory or degenerative diseases of the joints of the hands. A comparison was made between 99m-TcPP, xeroradiography and radiography. In a total of 630 different joints 237 positive scans, 132 positive roentgenograms and 183 positive xeroradiograms were obtained. In the early stage of inflammatory joint disease only the scintigraphic results are positive, in the later stage 99mTcPP-imaging was less sensitive than xeroradiography and radiography. The reason for the increased concentration at the site of acute joint inflammation is probably the increased uptake in the soft tissues of the joints. The best time for scanning after the application of 99m-TcPP seemed to be at 3 hours.", "contents": "[99mTc-polyphosphate scintigraphy of joints, xeroradiography and radiography of hands]. Joint imaging with 99m-Tc-Polyphosphate was carried out in 21 patients having inflammatory or degenerative diseases of the joints of the hands. A comparison was made between 99m-TcPP, xeroradiography and radiography. In a total of 630 different joints 237 positive scans, 132 positive roentgenograms and 183 positive xeroradiograms were obtained. In the early stage of inflammatory joint disease only the scintigraphic results are positive, in the later stage 99mTcPP-imaging was less sensitive than xeroradiography and radiography. The reason for the increased concentration at the site of acute joint inflammation is probably the increased uptake in the soft tissues of the joints. The best time for scanning after the application of 99m-TcPP seemed to be at 3 hours."}
{"id": "PMID:1078732", "title": "Mitogen-induced blastogenic responses of lymphocytes from marihuana smokers.", "content": "Blastogenic responses in vitro to phytohemagglutinin and pokeweed mitogen were examined in microcultures of peripheral blood lymphocytes from a group of 12 healthy, long-term marihuana smokers and a group of matched control subjects. With either mitogen, no significant difference in cellular incorporation of (3H)thymidine was noted between the groups. These results were interpreted to indicate that the functional status of blood lymphocytes was not altered by long-term smoking of marihuana.", "contents": "Mitogen-induced blastogenic responses of lymphocytes from marihuana smokers. Blastogenic responses in vitro to phytohemagglutinin and pokeweed mitogen were examined in microcultures of peripheral blood lymphocytes from a group of 12 healthy, long-term marihuana smokers and a group of matched control subjects. With either mitogen, no significant difference in cellular incorporation of (3H)thymidine was noted between the groups. These results were interpreted to indicate that the functional status of blood lymphocytes was not altered by long-term smoking of marihuana."}
{"id": "PMID:1078743", "title": "Complement-fixing and lymphocytotoxic antibodies in serum of pregnant women at delivery II. Clinical observations I.", "content": "Sera from 2,008 pregnant women were examined for the presence of complement-fixing platelet antibodies (CFPAb) and lymphocytotoxic antibodies (LCTAb). Women with antibodies in their serum were compared with women without antibodies. No clinical significance of CFPAb or LCTAb was found as regards pre-eclampsia, arterial hypertension, albuminuria, oedema, glucosuria, premature delivery, spontaneous onset of labor and abortion.", "contents": "Complement-fixing and lymphocytotoxic antibodies in serum of pregnant women at delivery II. Clinical observations I. Sera from 2,008 pregnant women were examined for the presence of complement-fixing platelet antibodies (CFPAb) and lymphocytotoxic antibodies (LCTAb). Women with antibodies in their serum were compared with women without antibodies. No clinical significance of CFPAb or LCTAb was found as regards pre-eclampsia, arterial hypertension, albuminuria, oedema, glucosuria, premature delivery, spontaneous onset of labor and abortion."}
{"id": "PMID:1078746", "title": "Coronary artery surgery for recurrent ventricular arrhythmias in patients with variant angina.", "content": "Two patients with Prinzmetal's variant angina had recurrent episodes of resting chest pain, ST segment elevation, QRS widening, ventricular tachycardia, and ventricular fibrillation. These eqisodes were unresponsive to medical therapy including lidocaine, procaine amide, and quinidine sulfate. Coronary arteriography revealed severe obstructive coronary artery disease, involving more than one coronary artery, in both patients. Aorticocoronary saphenous vein grafts were utilized to bypass significant disease in each patient. In one patient blood flow through the grafts was measured at 90 and 65 ml per minute, respectively, at operation and patent grafts were demonstrated six months postoperatively. Neither patient has had recurrence of chest pain or evidence of ventricular tachycardia at one year or 2 1/2 years postoperatively. Postoperative resting and maximal exercise ECG's are normal. Coronary artery surgery may be an effective method of therapy for ischemic ventricular tachycardia when medical therapy fails.", "contents": "Coronary artery surgery for recurrent ventricular arrhythmias in patients with variant angina. Two patients with Prinzmetal's variant angina had recurrent episodes of resting chest pain, ST segment elevation, QRS widening, ventricular tachycardia, and ventricular fibrillation. These eqisodes were unresponsive to medical therapy including lidocaine, procaine amide, and quinidine sulfate. Coronary arteriography revealed severe obstructive coronary artery disease, involving more than one coronary artery, in both patients. Aorticocoronary saphenous vein grafts were utilized to bypass significant disease in each patient. In one patient blood flow through the grafts was measured at 90 and 65 ml per minute, respectively, at operation and patent grafts were demonstrated six months postoperatively. Neither patient has had recurrence of chest pain or evidence of ventricular tachycardia at one year or 2 1/2 years postoperatively. Postoperative resting and maximal exercise ECG's are normal. Coronary artery surgery may be an effective method of therapy for ischemic ventricular tachycardia when medical therapy fails."}
{"id": "PMID:1078748", "title": "Evaluation of ristocetin-Willebrand factor assay and ristocetin-induced platelet aggregation.", "content": "Normal subjects, patients with various bleeding disorders, and patients with von Willebrand's disease were studied. All patients with von Willebrand's disease had decreased levels of ristocetin-Willebrand factor (range, 0 to 41%) as compared with all other subjects (range, 79 to 202%). Ristocetin-induced platelet aggregation of platelet-rich plasma was abnormal in all patients with von Willebrand's disease tested, and it was possible to correct this abnormal response by addition of normal platelet-poor plasma. Abnormal ristocetin-induced platelet aggregation was seen in patients with intrinsic platelet disorders or, on some occasions, in normal patients who had ingested aspirin. Ristocetin-induced platelet aggregation is not diagnostic, but it may be useful as a simple screening test for patients with possible von Willebrand's disease. In conjunction with other tests, the assay for ristocetin-Willebrand factor will be useful in diagnosis and evaluation of these patients.", "contents": "Evaluation of ristocetin-Willebrand factor assay and ristocetin-induced platelet aggregation. Normal subjects, patients with various bleeding disorders, and patients with von Willebrand's disease were studied. All patients with von Willebrand's disease had decreased levels of ristocetin-Willebrand factor (range, 0 to 41%) as compared with all other subjects (range, 79 to 202%). Ristocetin-induced platelet aggregation of platelet-rich plasma was abnormal in all patients with von Willebrand's disease tested, and it was possible to correct this abnormal response by addition of normal platelet-poor plasma. Abnormal ristocetin-induced platelet aggregation was seen in patients with intrinsic platelet disorders or, on some occasions, in normal patients who had ingested aspirin. Ristocetin-induced platelet aggregation is not diagnostic, but it may be useful as a simple screening test for patients with possible von Willebrand's disease. In conjunction with other tests, the assay for ristocetin-Willebrand factor will be useful in diagnosis and evaluation of these patients."}
{"id": "PMID:1078749", "title": "Serum trypsin inhibitory capacity and Pi phenotypes. I. Methods and control values.", "content": "Serum trypsin inhibitory capacity determinations are of considerable value in detecting genetically determined types of obstructive pulmonary disease and hepatic disease. These determinations must frequently be followed by determination of protease inhibitor (Pi) phenotype in order to confirm the diagnosis. Piphenotyping has been a specialized and time-consuming procedure, and suggested improvements in the methodology and technics may make it more generally applicable as a clinical laboratory procedure. The prevalence of phenotypes other than MM in a group of 700 control sera from blood donors is reported as a baseline to evaluate typically American populations of mixed ethnic and racial characteristics. There are suggestive differences in prevalences of S and Z genes relating to ethnic stock and racial groups. It is important when comparing the prevalences of S and Z genes in diseasedpopulations to use control groups of similar ethnic and racial compositions. Pi phenotyping is a necessary laboratory procedure in the diagnosis of certain forms of genetically determined chronic obstructive pulmonary disease and hepatic disease. The distributions of all serum protease inhibitory capacity values and those for S and Z Pi phenotypes are shown.", "contents": "Serum trypsin inhibitory capacity and Pi phenotypes. I. Methods and control values. Serum trypsin inhibitory capacity determinations are of considerable value in detecting genetically determined types of obstructive pulmonary disease and hepatic disease. These determinations must frequently be followed by determination of protease inhibitor (Pi) phenotype in order to confirm the diagnosis. Piphenotyping has been a specialized and time-consuming procedure, and suggested improvements in the methodology and technics may make it more generally applicable as a clinical laboratory procedure. The prevalence of phenotypes other than MM in a group of 700 control sera from blood donors is reported as a baseline to evaluate typically American populations of mixed ethnic and racial characteristics. There are suggestive differences in prevalences of S and Z genes relating to ethnic stock and racial groups. It is important when comparing the prevalences of S and Z genes in diseasedpopulations to use control groups of similar ethnic and racial compositions. Pi phenotyping is a necessary laboratory procedure in the diagnosis of certain forms of genetically determined chronic obstructive pulmonary disease and hepatic disease. The distributions of all serum protease inhibitory capacity values and those for S and Z Pi phenotypes are shown."}
{"id": "PMID:1078751", "title": "Enhanced rate of progression of coronary artery disease following aortocoronary saphenous vein bypass surgery.", "content": "To evaluate the fate of the boronary arteries after aortocoronary bypass, 40 patients underwent serial selective coronary angiographic studies 1 year apart, and the frequency of progression of coronary artery disease was estimated. Thirty-two had saphenous vein bypass surgery after the first procedure, six had Vineberg operations, and two had no interim operation. In each patient, the right, left, anterior descending and circumflex coronary arteries (including their branches) were separately evaluated. Progressive narrowing was evident in 31 of 50 (62 per cent) bypassed vessels and in only 11 to 113 (9.7 per cent) nonbypassed arteries (p less than 0.001). Coronary arteries with moderate to severe obstruction initially (50 to 99 per cent occluded) manifested progressive diseasee more frequently (33 of 70 arteries) than did arteries that were normal or mildly narrowed initially (4 of 71) (p less than 0.001. Considering only those vessels with 50 to 99 per cent obstruction initially, 27 of 35 (77 per cent) of the bypassed arteries and only 6 of 35 (17 per cent) of the nonbypassed arteries showed progression (p less than 0.001). We conclude that moderately or severely narrowed coronary arteries are more likely to show progressive narrowing than normal or mildly obstructed ones and that progression of coronary disease is greater in bypassed vessels than in nonbypassed vessels. In view of potential graft closure, the implications of these findings must be considered in selecting patients for aortocoronary bypass.", "contents": "Enhanced rate of progression of coronary artery disease following aortocoronary saphenous vein bypass surgery. To evaluate the fate of the boronary arteries after aortocoronary bypass, 40 patients underwent serial selective coronary angiographic studies 1 year apart, and the frequency of progression of coronary artery disease was estimated. Thirty-two had saphenous vein bypass surgery after the first procedure, six had Vineberg operations, and two had no interim operation. In each patient, the right, left, anterior descending and circumflex coronary arteries (including their branches) were separately evaluated. Progressive narrowing was evident in 31 of 50 (62 per cent) bypassed vessels and in only 11 to 113 (9.7 per cent) nonbypassed arteries (p less than 0.001). Coronary arteries with moderate to severe obstruction initially (50 to 99 per cent occluded) manifested progressive diseasee more frequently (33 of 70 arteries) than did arteries that were normal or mildly narrowed initially (4 of 71) (p less than 0.001. Considering only those vessels with 50 to 99 per cent obstruction initially, 27 of 35 (77 per cent) of the bypassed arteries and only 6 of 35 (17 per cent) of the nonbypassed arteries showed progression (p less than 0.001). We conclude that moderately or severely narrowed coronary arteries are more likely to show progressive narrowing than normal or mildly obstructed ones and that progression of coronary disease is greater in bypassed vessels than in nonbypassed vessels. In view of potential graft closure, the implications of these findings must be considered in selecting patients for aortocoronary bypass."}
{"id": "PMID:1078752", "title": "Coronary bypass surgery for unstable angina pectoris. Clinical follow-up and results of postoperative treadmill electrocardiograms.", "content": "The first 81 patients who underwent coronary artery bypass surgery at Stanford University Hospital for unstable angina pectoris have been followed up for an average of 18 months. The over-all surgical mortality was 8.6 per cent (seven patients). There have been no operative deaths in last 32 patients, which may be due to over 75 per cent of these patients being stabilized on intensive medical therapy from 24 to 72 hours before study or surgery. There was a 16 per cent (13 patients) perioperative and 15 per cent (12 patients) late incidence of myocardial infarction. Of 74 patients who survived the initial operation 2 died 2 and 3 months postoperatively. Good or complete relief from pain was obtained in 94 per cent (70 patients) of the survivors. Of 57 longterm survivors tested, 49 per cent (28 patients) had a definite ischemic response to treadmill exercise testing. This may reflect the severe nature of the occlusive coronary disease or mechanisms other than increased coronary flow being responsible for the relief of pain. Although coronary bypass surgery appears to be effective in relieving the pain of patients with unstable angina pectoris, the 18 month average follow-up indicates that the incidence of myocardial infarction in surgically treated patients is comparable to that in medically treated patients.", "contents": "Coronary bypass surgery for unstable angina pectoris. Clinical follow-up and results of postoperative treadmill electrocardiograms. The first 81 patients who underwent coronary artery bypass surgery at Stanford University Hospital for unstable angina pectoris have been followed up for an average of 18 months. The over-all surgical mortality was 8.6 per cent (seven patients). There have been no operative deaths in last 32 patients, which may be due to over 75 per cent of these patients being stabilized on intensive medical therapy from 24 to 72 hours before study or surgery. There was a 16 per cent (13 patients) perioperative and 15 per cent (12 patients) late incidence of myocardial infarction. Of 74 patients who survived the initial operation 2 died 2 and 3 months postoperatively. Good or complete relief from pain was obtained in 94 per cent (70 patients) of the survivors. Of 57 longterm survivors tested, 49 per cent (28 patients) had a definite ischemic response to treadmill exercise testing. This may reflect the severe nature of the occlusive coronary disease or mechanisms other than increased coronary flow being responsible for the relief of pain. Although coronary bypass surgery appears to be effective in relieving the pain of patients with unstable angina pectoris, the 18 month average follow-up indicates that the incidence of myocardial infarction in surgically treated patients is comparable to that in medically treated patients."}
{"id": "PMID:1078753", "title": "Immunologic and morphologic studies of T cell lymphoma.", "content": "Thymic-independent (B) lymphocytes, thymic-independent (T) lymphocytes and histiocytes may be distinguished by the presence of certain surface markers. In addition B and T lymphocytes have been reported to show distinctive surface architecture by scanning electron microscopy. Neoplastic cells from a lymph node and cerebrospinal fluid of a patient with a diffuse malignant lymphoma of the poorly differentiated lymphocytic type were examined in frozen sections and cell suspensions for the presence of surface immunoglobulin and the antigen-antibody-complement (IgMEAC) receptor of B lymphocytes, the presence of the cytophilic antibody (IgGEA) receptor of histiocytes and the ability to form nonimmune rosettes with sheep red blood cells (E) characteristic of T lymphocytes. Cells from the lymph node were also studied by scanning electron microscopy. The majority of neoplastic cells from the lymph node and cerebrospinal fluid formed rosettes with E, but lacked surface immunoglobulin and failed to bind IgMEAC or IgGEA. By scanning electron microscopy the neoplastic cells, although larger in diameter, showed surface architecture similar to normal lymphocytes with a varying number of surface microvilli. These studies suggest that the malignant cells of this lymphoma are of thymic type.", "contents": "Immunologic and morphologic studies of T cell lymphoma. Thymic-independent (B) lymphocytes, thymic-independent (T) lymphocytes and histiocytes may be distinguished by the presence of certain surface markers. In addition B and T lymphocytes have been reported to show distinctive surface architecture by scanning electron microscopy. Neoplastic cells from a lymph node and cerebrospinal fluid of a patient with a diffuse malignant lymphoma of the poorly differentiated lymphocytic type were examined in frozen sections and cell suspensions for the presence of surface immunoglobulin and the antigen-antibody-complement (IgMEAC) receptor of B lymphocytes, the presence of the cytophilic antibody (IgGEA) receptor of histiocytes and the ability to form nonimmune rosettes with sheep red blood cells (E) characteristic of T lymphocytes. Cells from the lymph node were also studied by scanning electron microscopy. The majority of neoplastic cells from the lymph node and cerebrospinal fluid formed rosettes with E, but lacked surface immunoglobulin and failed to bind IgMEAC or IgGEA. By scanning electron microscopy the neoplastic cells, although larger in diameter, showed surface architecture similar to normal lymphocytes with a varying number of surface microvilli. These studies suggest that the malignant cells of this lymphoma are of thymic type."}
{"id": "PMID:1078754", "title": "Hemolytic anemia associated with multiple autoantibodies and low serum complement.", "content": "A 37 year old woman with extravascular hemolytic anemia had a positive Monospot test associated with positive antiglobulin and anticomplement Coombs' tests, cold agglutinins and warm autoantibodies. IgG-kappa (k) antibodies, which reacted with all panel red cells at 37 degrees C, were eluted from her circulating red cells. However, neither immunoglobulins nor C3 was detected after her serum was adsorbed with heterologous red cell stroma at 37 degrees C and eluted at the same temperature in glycine buffer. In contrast, IgM-kappa and IgM-lambda (lambda), IgG-3-kappa, IgG4-lambda, IgA-lambda and C3 were eluted at 37 degrees C from heterologous red cell stroma after adsorption with her serum at 0 degrees C. Thus, antibodies of several types, which were present in the patient's serum, reacted optimally with red cell antigens at low temperature. Cold-reactive IgG3-kappa antibodies, which also capable of interacting with red cells at 37 degrees C, probably accounted for the IgG-kappa antibodies eluted from the patient's circulating red cells. The patient's serum C4 titers were decreased, with low normal to moderately depressed C3 and low normal C5, indicating that the anti-red cell IgM and/or IgG3-kappa antibodies probably fixed complement. A localized cold stress test resulted in a transient increase in plasma hemoglobin and a decrease in serum C3 titer. These findings, and the beneficial clinical response obtained with small doses of prednisone, suggest that both the cold-reactive antibodies and the IgG-kappa on circulating red cells were pathophysiologically significant. This is the first report of a patient with multiple red cell autoantibodies in whom serum complement component titers were determined in conjunction with characterization of the anti-red cell immunoglobulins. Subclinical infectious mononucleosis may have preceded the prolonged hemolytic episode. Clinical evidence of systemic lupus erythematosus has not appeared.", "contents": "Hemolytic anemia associated with multiple autoantibodies and low serum complement. A 37 year old woman with extravascular hemolytic anemia had a positive Monospot test associated with positive antiglobulin and anticomplement Coombs' tests, cold agglutinins and warm autoantibodies. IgG-kappa (k) antibodies, which reacted with all panel red cells at 37 degrees C, were eluted from her circulating red cells. However, neither immunoglobulins nor C3 was detected after her serum was adsorbed with heterologous red cell stroma at 37 degrees C and eluted at the same temperature in glycine buffer. In contrast, IgM-kappa and IgM-lambda (lambda), IgG-3-kappa, IgG4-lambda, IgA-lambda and C3 were eluted at 37 degrees C from heterologous red cell stroma after adsorption with her serum at 0 degrees C. Thus, antibodies of several types, which were present in the patient's serum, reacted optimally with red cell antigens at low temperature. Cold-reactive IgG3-kappa antibodies, which also capable of interacting with red cells at 37 degrees C, probably accounted for the IgG-kappa antibodies eluted from the patient's circulating red cells. The patient's serum C4 titers were decreased, with low normal to moderately depressed C3 and low normal C5, indicating that the anti-red cell IgM and/or IgG3-kappa antibodies probably fixed complement. A localized cold stress test resulted in a transient increase in plasma hemoglobin and a decrease in serum C3 titer. These findings, and the beneficial clinical response obtained with small doses of prednisone, suggest that both the cold-reactive antibodies and the IgG-kappa on circulating red cells were pathophysiologically significant. This is the first report of a patient with multiple red cell autoantibodies in whom serum complement component titers were determined in conjunction with characterization of the anti-red cell immunoglobulins. Subclinical infectious mononucleosis may have preceded the prolonged hemolytic episode. Clinical evidence of systemic lupus erythematosus has not appeared."}
{"id": "PMID:1078755", "title": "Anosmia and hypogonadism with ovarian mosaicism.", "content": "An 18-year-old woman with anosmia and hypogonadotropic hypogonadism is presented. In addition endocrinologic evaluation revealed an apparent deficiency in pituitary growth hormone secretion in response to hypoglycemia and an ovarian insensitivity to exogenous gonadotropins. The ovaries, which on histologic examination appeared to be normal, upon karyotyping showed a chromosomal mosaicism, probably 46, XX/47, XXF+.", "contents": "Anosmia and hypogonadism with ovarian mosaicism. An 18-year-old woman with anosmia and hypogonadotropic hypogonadism is presented. In addition endocrinologic evaluation revealed an apparent deficiency in pituitary growth hormone secretion in response to hypoglycemia and an ovarian insensitivity to exogenous gonadotropins. The ovaries, which on histologic examination appeared to be normal, upon karyotyping showed a chromosomal mosaicism, probably 46, XX/47, XXF+."}
{"id": "PMID:1078756", "title": "Response of active transport of ions and spontaneous water flux to osmotic gradients in gastric mucosa.", "content": "The effects of symmetric changes of the mucosal and serosal bathing solution on cell water content, net ion flux, and net water movement were studied in the isolated frog gastric mucosa. Similar to transmucosal concentration gradients that induce water movement and changes in cell water content, symmetric osmolality changes of the bathing solutions also produce changes in these functional parameters. Thus, increments from 165 to 286 mosmol/kg water in the osmolality of both solutions reduce cell water content from 2.37 plus or minus 0.12 to 1.30 plus or minus 0.20 ml/g wt, the net ion flux (acid secretion plus short-circuit current) from 4.83 plus or minus 0.36 to 3.44 plus or minus 0.26 mueq/cm2 per h, and the net water flux from 10.6 plus or minus 1.1 to 2.4 plus or minus 1.2 mul/cm2 per h. These osmotically induced flux changes of water and ions must be considered when osmotic gradients are being used to generate and to evaluate water movement across the gastric mucosa.", "contents": "Response of active transport of ions and spontaneous water flux to osmotic gradients in gastric mucosa. The effects of symmetric changes of the mucosal and serosal bathing solution on cell water content, net ion flux, and net water movement were studied in the isolated frog gastric mucosa. Similar to transmucosal concentration gradients that induce water movement and changes in cell water content, symmetric osmolality changes of the bathing solutions also produce changes in these functional parameters. Thus, increments from 165 to 286 mosmol/kg water in the osmolality of both solutions reduce cell water content from 2.37 plus or minus 0.12 to 1.30 plus or minus 0.20 ml/g wt, the net ion flux (acid secretion plus short-circuit current) from 4.83 plus or minus 0.36 to 3.44 plus or minus 0.26 mueq/cm2 per h, and the net water flux from 10.6 plus or minus 1.1 to 2.4 plus or minus 1.2 mul/cm2 per h. These osmotically induced flux changes of water and ions must be considered when osmotic gradients are being used to generate and to evaluate water movement across the gastric mucosa."}
{"id": "PMID:1078757", "title": "Chloride transport through the n onshort-circuited isolated skin of Rana esculenta.", "content": "The effects of acetazolamide (Diamox), ouabain, and amiloride on chloride movements through the isolated, nonshort-circuited Rana esculenta skin were investigated at different external NaCl concentrations. From hyposmotic solutions of low NaCl concentration (2 mM), two principal components of the chloride uptake may be distinguished: one dependent on the osmotic water flux and the other inhibited by Diamox but not by ouabain, amiloride, or removal of sodium from the external solution. Chloride uptake from a Ringer solution is always inhibited when either the sodium transport is blocked with amiloride or ouabain or when sodium is absent from the external solution. It can be concluded that the predominant component of the chloride transport mechanism when the external solution is concentrated (Ringer) is a sodium-dependent one. When chloride is absorbed from a solution of low concentration, however, a component predominated which is independent of sodium transport. It is suggested that the latter mechanism should be capable of exchanging chloride against endogenous base as in the in vivo skin.", "contents": "Chloride transport through the n onshort-circuited isolated skin of Rana esculenta. The effects of acetazolamide (Diamox), ouabain, and amiloride on chloride movements through the isolated, nonshort-circuited Rana esculenta skin were investigated at different external NaCl concentrations. From hyposmotic solutions of low NaCl concentration (2 mM), two principal components of the chloride uptake may be distinguished: one dependent on the osmotic water flux and the other inhibited by Diamox but not by ouabain, amiloride, or removal of sodium from the external solution. Chloride uptake from a Ringer solution is always inhibited when either the sodium transport is blocked with amiloride or ouabain or when sodium is absent from the external solution. It can be concluded that the predominant component of the chloride transport mechanism when the external solution is concentrated (Ringer) is a sodium-dependent one. When chloride is absorbed from a solution of low concentration, however, a component predominated which is independent of sodium transport. It is suggested that the latter mechanism should be capable of exchanging chloride against endogenous base as in the in vivo skin."}
{"id": "PMID:1078758", "title": "Two-year follow-up of angina pectoris: medical or surgical therapy.", "content": "The effects of bypass graft surgery versus continued medical management in 40 patients with stable angina were evaluated at 2 years: 17 of 20 surgical patients (85%) and 18 of 20 medical patients (90%) were alive, and 5 of 20 surgical patients (25%) and 2 of 20 medical patients (10%) had developed myocardial infarction; 8 of 17 surgical patients (47%) and 4 of 18 medical patients (22%) had no angina, and 13 of 17 surgical patients (76%) and 9 of 18 medical patients (50%) had no angina or greater than 25% increase in exercise time until angina. No statistically significant differences were demonstrated between the medical and surgical groups for all variables submitted to statistical analysis.", "contents": "Two-year follow-up of angina pectoris: medical or surgical therapy. The effects of bypass graft surgery versus continued medical management in 40 patients with stable angina were evaluated at 2 years: 17 of 20 surgical patients (85%) and 18 of 20 medical patients (90%) were alive, and 5 of 20 surgical patients (25%) and 2 of 20 medical patients (10%) had developed myocardial infarction; 8 of 17 surgical patients (47%) and 4 of 18 medical patients (22%) had no angina, and 13 of 17 surgical patients (76%) and 9 of 18 medical patients (50%) had no angina or greater than 25% increase in exercise time until angina. No statistically significant differences were demonstrated between the medical and surgical groups for all variables submitted to statistical analysis."}
{"id": "PMID:1078759", "title": "Adult toxoplasmosis presenting as polymyositis and cerebellar ataxia.", "content": "A patient with toxoplasmosis had cerebellar, spinal cord, nerve root, and skeletal muscle symptoms. Toxoplasma gondii was isolated from skeletal muscle, and toxoplasma antibody titers were persistently elevated. The disease has been recurrent during a 3-year period with only temporary response to specific antimicrobial therapy.", "contents": "Adult toxoplasmosis presenting as polymyositis and cerebellar ataxia. A patient with toxoplasmosis had cerebellar, spinal cord, nerve root, and skeletal muscle symptoms. Toxoplasma gondii was isolated from skeletal muscle, and toxoplasma antibody titers were persistently elevated. The disease has been recurrent during a 3-year period with only temporary response to specific antimicrobial therapy."}
{"id": "PMID:1078762", "title": "Dermatomyositis in six patients without initial muscle involvement.", "content": "Six patients initially had the characteristic skin eruption of dermatomyositis but no other evidence of the disease. They all later developed dermatomyositis. The clinical, laboratory, and pathological data suggest that whereas polymyositis may occur without skin involvement, the cutaneous features od dermatomyositis may be seen in the absence of detectable muscle disease. This variant of dermatomyositis occurs in children and adults but has not been found to be associated with internal malignant disease. The persistence of skin lesions despite systemic corticosteroid therapy is a poor prognostic sign. There were two patients with pulmonary fibrosis and dermatomyositis in this group. The presence of pulmonary fibrosis can contribute to a delay in diagnosis of dermatomyositis. Patients with pulmonary fibrosis had a poorer prognosis, despite moderate dosages of corticosteroids.", "contents": "Dermatomyositis in six patients without initial muscle involvement. Six patients initially had the characteristic skin eruption of dermatomyositis but no other evidence of the disease. They all later developed dermatomyositis. The clinical, laboratory, and pathological data suggest that whereas polymyositis may occur without skin involvement, the cutaneous features od dermatomyositis may be seen in the absence of detectable muscle disease. This variant of dermatomyositis occurs in children and adults but has not been found to be associated with internal malignant disease. The persistence of skin lesions despite systemic corticosteroid therapy is a poor prognostic sign. There were two patients with pulmonary fibrosis and dermatomyositis in this group. The presence of pulmonary fibrosis can contribute to a delay in diagnosis of dermatomyositis. Patients with pulmonary fibrosis had a poorer prognosis, despite moderate dosages of corticosteroids."}
{"id": "PMID:1078763", "title": "\"No-vent\" technique of coronary artery bypass.", "content": "Isolated coronary artery bypass grafting was performed in 100 patients without the use of a left ventricular vent. Distention of the left ventricle did not occur. There was 1 late death unrelated to this technique. Vents in the left heart are not required under most circumstances.", "contents": "\"No-vent\" technique of coronary artery bypass. Isolated coronary artery bypass grafting was performed in 100 patients without the use of a left ventricular vent. Distention of the left ventricle did not occur. There was 1 late death unrelated to this technique. Vents in the left heart are not required under most circumstances."}
{"id": "PMID:1078769", "title": "A new application of counterpulsation: safer laparotomy after recent myocardial infarction.", "content": "A 56-year old man suffered severe gastrointestinal tract bleeding five days following an anterior myocardial infraction. Nonoperative treatment consisting of gastric lavage, selective angiographic procedures on the gastric arteries with intra-arterial vasopressin injection (Pitressin), and massive blood replacement over a five-day period failed to halt hemorrhage. Institution of intra-aortic balloon pumping reversed the patient's hemodynamic deterioration and made possible a definitive operation. The successful outcome in this patient suggests that counterpulsation may play a useful role in lowering the formidable surgical mortality observed in patients with acute myocardial injury who require emergency noncardiac operations.", "contents": "A new application of counterpulsation: safer laparotomy after recent myocardial infarction. A 56-year old man suffered severe gastrointestinal tract bleeding five days following an anterior myocardial infraction. Nonoperative treatment consisting of gastric lavage, selective angiographic procedures on the gastric arteries with intra-arterial vasopressin injection (Pitressin), and massive blood replacement over a five-day period failed to halt hemorrhage. Institution of intra-aortic balloon pumping reversed the patient's hemodynamic deterioration and made possible a definitive operation. The successful outcome in this patient suggests that counterpulsation may play a useful role in lowering the formidable surgical mortality observed in patients with acute myocardial injury who require emergency noncardiac operations."}
{"id": "PMID:1078771", "title": "Multiple primary malignant neoplasms. A search for an immunogenetic basis.", "content": "The occurrence of multiple primary malignant neoplasms in single individuals is well documented. Although many hypotheses have been advanced to explain this occurrence, there has been no study to determine if a presumed \"increased susceptibility to cancer\" has an immunogenetic basis. We evaluated the cellular immunity and histocompatibility antigens of 42 patients who had had from two to four multiple primary malignant neoplasms. We failed to demonstrate a preexisting impairment of immunocompetence or abnormal HL-A antigen frequencies in these patients. The occurrence of multiple primary malignant neoplasms in related tissues, eg, lung/larynx/oral cavity, and the occurrence of successive primary malignant neoplasms at a time interval consistent with the patient's being cured of preceding malignant neoplasms suggest that multiple primary malignant neoplasms result from repetitive induction by the same or similar etiologic factors in patients who are cured after treatment of the first malignant neoplasm.", "contents": "Multiple primary malignant neoplasms. A search for an immunogenetic basis. The occurrence of multiple primary malignant neoplasms in single individuals is well documented. Although many hypotheses have been advanced to explain this occurrence, there has been no study to determine if a presumed \"increased susceptibility to cancer\" has an immunogenetic basis. We evaluated the cellular immunity and histocompatibility antigens of 42 patients who had had from two to four multiple primary malignant neoplasms. We failed to demonstrate a preexisting impairment of immunocompetence or abnormal HL-A antigen frequencies in these patients. The occurrence of multiple primary malignant neoplasms in related tissues, eg, lung/larynx/oral cavity, and the occurrence of successive primary malignant neoplasms at a time interval consistent with the patient's being cured of preceding malignant neoplasms suggest that multiple primary malignant neoplasms result from repetitive induction by the same or similar etiologic factors in patients who are cured after treatment of the first malignant neoplasm."}
{"id": "PMID:1078772", "title": "Supraceliac pseudoaneurysm of abdominal aorta. Staged repair employing preliminary axillary-femoral bypass.", "content": "The fifth successfully treated pseudoaneurysm of the visceral branch segment of abdominal aorta emphasizes individualized management of such complex lesions. Staged operative management of a supraceliac pseudoaneurysm by preliminary axillary-femoral bypass minimized potential intraoperative hazards. Visceral protection during aortic occlusion was simplified by axillary-femoral perfusion.", "contents": "Supraceliac pseudoaneurysm of abdominal aorta. Staged repair employing preliminary axillary-femoral bypass. The fifth successfully treated pseudoaneurysm of the visceral branch segment of abdominal aorta emphasizes individualized management of such complex lesions. Staged operative management of a supraceliac pseudoaneurysm by preliminary axillary-femoral bypass minimized potential intraoperative hazards. Visceral protection during aortic occlusion was simplified by axillary-femoral perfusion."}
{"id": "PMID:1078773", "title": "Emergency and urgent operations for ulcerative colitis. The procedure of choice.", "content": "Eighty consecutive emergency and urgent colectomies for ulcerative colitis were performed. One-stage total proctocolectomy was performed in 37 patients, with a 9.1% mortality; ileostomy with subtotal colectomy was performed in 43, with a 7.0% mortality. The overall mortality was 7.5%. Postoperative morbidity after total proctocolectomy (mean postoperative hospitalization, 27.6 days; nonseptic complication rate, 29.4%; septic complication rate, 29.4%) was not substantially different from that after subtotal colectomy (postoperative hospitalization, 33.3 days; nonseptic complications, 45.0%; septic complications, 35.0%). Survivors of subtotal colectomy required abdominal-perineal resection of the colorectal remnant in 75.7% of patients, and no patient had successful subsequent ileorectal anastomosis. It is suggested that one-stage total proctocolectomy be adopted as the surgical procedure of choice in emergency or urgent operations for ulcerative colitis.", "contents": "Emergency and urgent operations for ulcerative colitis. The procedure of choice. Eighty consecutive emergency and urgent colectomies for ulcerative colitis were performed. One-stage total proctocolectomy was performed in 37 patients, with a 9.1% mortality; ileostomy with subtotal colectomy was performed in 43, with a 7.0% mortality. The overall mortality was 7.5%. Postoperative morbidity after total proctocolectomy (mean postoperative hospitalization, 27.6 days; nonseptic complication rate, 29.4%; septic complication rate, 29.4%) was not substantially different from that after subtotal colectomy (postoperative hospitalization, 33.3 days; nonseptic complications, 45.0%; septic complications, 35.0%). Survivors of subtotal colectomy required abdominal-perineal resection of the colorectal remnant in 75.7% of patients, and no patient had successful subsequent ileorectal anastomosis. It is suggested that one-stage total proctocolectomy be adopted as the surgical procedure of choice in emergency or urgent operations for ulcerative colitis."}
{"id": "PMID:1078774", "title": "Superficial microcystic corneal dystrophy.", "content": "Microcystic corneal dystrophy was first described in 1964. Since then, several reports have elaborated on the original findings plus other associated corneal changes. The cause is unknown, but pathological studies have been done in several cases. The corneal changes are subtle and easy to miss on routine examination. This study of 17 patients was undertaken to further evaluate the signs and symptoms of this abnormality and to emphasize techniques of examination. Detailed clinical and laboratory evaluations of tear function were performed. No definite association between tear function and the corneal changes could be determined. In this group of patients, the process seemed benign with minimal symptoms.", "contents": "Superficial microcystic corneal dystrophy. Microcystic corneal dystrophy was first described in 1964. Since then, several reports have elaborated on the original findings plus other associated corneal changes. The cause is unknown, but pathological studies have been done in several cases. The corneal changes are subtle and easy to miss on routine examination. This study of 17 patients was undertaken to further evaluate the signs and symptoms of this abnormality and to emphasize techniques of examination. Detailed clinical and laboratory evaluations of tear function were performed. No definite association between tear function and the corneal changes could be determined. In this group of patients, the process seemed benign with minimal symptoms."}
{"id": "PMID:1078775", "title": "Dissecting aneurysms of coronary arteries.", "content": "A case of dissecting aneurysms of coronary arteries caused by cardiac surgery was found. Review discloses that most coronary arteries arise spontaneously, occur more often in women than men, frequently in the postpartum period, and are characterized by the sudden onset of acute chest pain in a previously well middle aged patient who abruptly collapses and dies. The anterior descending branch of the left coronary artery is most frequently affected, the dissection usually involves the first portion of that single vessel, and the lesion appears amenable to bypass venous grafting. While the cause is unknown, the frequency of pregnancy, the loosening effect of that condition on connective tissue, and the occasional finding of medial degeneration of the arterial wall suggest cystic medial necrosis as an important etiologic consideration. This case report recommends gentle handling of coronary arteries in surgical procedures.", "contents": "Dissecting aneurysms of coronary arteries. A case of dissecting aneurysms of coronary arteries caused by cardiac surgery was found. Review discloses that most coronary arteries arise spontaneously, occur more often in women than men, frequently in the postpartum period, and are characterized by the sudden onset of acute chest pain in a previously well middle aged patient who abruptly collapses and dies. The anterior descending branch of the left coronary artery is most frequently affected, the dissection usually involves the first portion of that single vessel, and the lesion appears amenable to bypass venous grafting. While the cause is unknown, the frequency of pregnancy, the loosening effect of that condition on connective tissue, and the occasional finding of medial degeneration of the arterial wall suggest cystic medial necrosis as an important etiologic consideration. This case report recommends gentle handling of coronary arteries in surgical procedures."}
{"id": "PMID:1078776", "title": "Influence of head orientation on visually induced pitch and roll sensation.", "content": "Observers viewing rotating scenes in their periphery frequently experience self-motion in the opposite direction. A full field (360 degrees) flight simulator projection system was used to investigate the sensations resulting from pitch, roll, and yaw stimuli at various head orientations. Steady yaw rate (circularvection) and development of a constant roll tilt angle, for the head erect and constant velocity yaw and roll stimuli, confirmed previous reports. Pitch stimuli also were found to produce a sensation of tilting to a steady pitch angle, which was much stronger for pitch forward than backward. Pitch and roll effects were strongly dependent on head position, increasing for the head rolled 90 degrees to the side or inverted, and decreasing for the head pitched 25 degrees forward. These results support a hypothesis that visually induced tilt is limited by conflict with otolith information.", "contents": "Influence of head orientation on visually induced pitch and roll sensation. Observers viewing rotating scenes in their periphery frequently experience self-motion in the opposite direction. A full field (360 degrees) flight simulator projection system was used to investigate the sensations resulting from pitch, roll, and yaw stimuli at various head orientations. Steady yaw rate (circularvection) and development of a constant roll tilt angle, for the head erect and constant velocity yaw and roll stimuli, confirmed previous reports. Pitch stimuli also were found to produce a sensation of tilting to a steady pitch angle, which was much stronger for pitch forward than backward. Pitch and roll effects were strongly dependent on head position, increasing for the head rolled 90 degrees to the side or inverted, and decreasing for the head pitched 25 degrees forward. These results support a hypothesis that visually induced tilt is limited by conflict with otolith information."}
{"id": "PMID:1078779", "title": "Abnormalities of factor VIII and platelet aggregation--use of ristocetin in diagnosing the von Willebrand syndrome.", "content": "Ristocetin was used to study platelet aggregation in platelet-rich plasma and to assay the von Willebrand factor activity of factor VIII (VIII-VWF). Ristocetin-induced platelet aggregation (RIPA) was decreased in 13 of 18 patients with von Willebrand's disease (VWD) who had decreased plasma levels of VIII-VWF. The five patients with normal RIPA appeared to have mild VWD but did not constitute a separate subclass. RIPA was also abnormal in some patients with intrinsic platelet defects, but in no case was the defect corrected by normal plasma. The latter type of correction appears to be specific for VWD. Aspirin ingestion inhibited the second phase of RIPA (at low concentrations of ristocetin only) but did not affect the initial phase of aggregation or the level of VIII-VWF. We also studied a group of patients who had both abnormalities of the factor VIII complex and intrinsic platelet defects, such as impaired collagen-induced aggregation, as well. The findings in these patients and in those with typical von Willebrand's disease appear to comprise a spectrum of disorders (the von Willebrand syndrome) in which some abnormality of the factor VIII complex is associated with impaired platelet function. At present, ristocetin would appear to be a useful reagent for evaluating patients with bleeding disorders and for studying patients with the von Willebrand syndrome.", "contents": "Abnormalities of factor VIII and platelet aggregation--use of ristocetin in diagnosing the von Willebrand syndrome. Ristocetin was used to study platelet aggregation in platelet-rich plasma and to assay the von Willebrand factor activity of factor VIII (VIII-VWF). Ristocetin-induced platelet aggregation (RIPA) was decreased in 13 of 18 patients with von Willebrand's disease (VWD) who had decreased plasma levels of VIII-VWF. The five patients with normal RIPA appeared to have mild VWD but did not constitute a separate subclass. RIPA was also abnormal in some patients with intrinsic platelet defects, but in no case was the defect corrected by normal plasma. The latter type of correction appears to be specific for VWD. Aspirin ingestion inhibited the second phase of RIPA (at low concentrations of ristocetin only) but did not affect the initial phase of aggregation or the level of VIII-VWF. We also studied a group of patients who had both abnormalities of the factor VIII complex and intrinsic platelet defects, such as impaired collagen-induced aggregation, as well. The findings in these patients and in those with typical von Willebrand's disease appear to comprise a spectrum of disorders (the von Willebrand syndrome) in which some abnormality of the factor VIII complex is associated with impaired platelet function. At present, ristocetin would appear to be a useful reagent for evaluating patients with bleeding disorders and for studying patients with the von Willebrand syndrome."}
{"id": "PMID:1078777", "title": "Rheumatoid nodulosis: an unusual variant of rheumatoid disease.", "content": "A middle-aged man developed multiple subcutaneous nodules associated with palindromic rheumatism. There was little evidence of synovitis; however multiple cyst-like intraosseous radiolucencies were noted. Nodules from two sites were histologically typical rheumatoid nodules. Subjective and objective improvement occurred during penicillamine therapy. This clincial presentation seems sufficiently distinctive to warrant characterization as a variant of rheumatoid disease termed by us: rheumatoid nodulosis.", "contents": "Rheumatoid nodulosis: an unusual variant of rheumatoid disease. A middle-aged man developed multiple subcutaneous nodules associated with palindromic rheumatism. There was little evidence of synovitis; however multiple cyst-like intraosseous radiolucencies were noted. Nodules from two sites were histologically typical rheumatoid nodules. Subjective and objective improvement occurred during penicillamine therapy. This clincial presentation seems sufficiently distinctive to warrant characterization as a variant of rheumatoid disease termed by us: rheumatoid nodulosis."}
{"id": "PMID:1078780", "title": "Abdominal approach for the ligation of bleeding oesophageal varices.", "content": "The conventional surgical treatment of bleeding oesophageal varices in the emergency situation is based upon the Boerema-Crile operation of transthoracic oesophagotomy and ligation of the varices. This and the other methods of treatment of this condition in current practice are discussed. Two cases are reported in which a transabdominal oesophagogastrotomy was used to approach the site of bleeding. This operation is described and the theoretical and practical advantages it appears to offer over the standard approach are considered.", "contents": "Abdominal approach for the ligation of bleeding oesophageal varices. The conventional surgical treatment of bleeding oesophageal varices in the emergency situation is based upon the Boerema-Crile operation of transthoracic oesophagotomy and ligation of the varices. This and the other methods of treatment of this condition in current practice are discussed. Two cases are reported in which a transabdominal oesophagogastrotomy was used to approach the site of bleeding. This operation is described and the theoretical and practical advantages it appears to offer over the standard approach are considered."}
{"id": "PMID:1078778", "title": "The effect of cyclophosphamide on B and T lymphocytes in patients with connective tissue diseases.", "content": "Absolute numbers of B (IgG-, IgM-, and IgA-staining) and T lymphocytes (sheep erythrocyte rosette-forming cells) were determined in patients with systemic lupus erythematosus and other connective tissue diseases in cyclophosphamide-treated and noncyclophosphamide-treated patients and in control subjects. In patients receiving cyclophosphamide, all three types of immunoglobulin-staining varieties of circulating B lymphocytes were significantly decreased. At the same time the circulating T lymphocytes were also significantly reduced. In patients with scleroderma treated with therapeutic doses of cyclophosphamide and studied sequentially, the reduction in B lymphocytes occurred first, with eventual depletion of both cell types. In 2 patients, an early rebound increase in T cells occurred followed by a marked reduction. These data indicate that the immunosuppressive effects of cyclophosphamide may be associated with a reduction in both cell types.", "contents": "The effect of cyclophosphamide on B and T lymphocytes in patients with connective tissue diseases. Absolute numbers of B (IgG-, IgM-, and IgA-staining) and T lymphocytes (sheep erythrocyte rosette-forming cells) were determined in patients with systemic lupus erythematosus and other connective tissue diseases in cyclophosphamide-treated and noncyclophosphamide-treated patients and in control subjects. In patients receiving cyclophosphamide, all three types of immunoglobulin-staining varieties of circulating B lymphocytes were significantly decreased. At the same time the circulating T lymphocytes were also significantly reduced. In patients with scleroderma treated with therapeutic doses of cyclophosphamide and studied sequentially, the reduction in B lymphocytes occurred first, with eventual depletion of both cell types. In 2 patients, an early rebound increase in T cells occurred followed by a marked reduction. These data indicate that the immunosuppressive effects of cyclophosphamide may be associated with a reduction in both cell types."}
{"id": "PMID:1078782", "title": "Pericarditis, pleural effusion, and pneumonitis with transient mitochondrial antibodies.", "content": "Four women with fever, arthromyalgias, pericarditis, pleural effusion, high erythrocyte sedimentation rates, and lymphopenia had mitochondrial antibodies in the serum in the absence of antinuclear antibody. Their illness lasted 5-12 weeks and the antibody test results became negative on remission. Absence of specific bacteriological findings, normal antistreptolysin O titres, resistance to antibiotics, and good response to steroids suggested that these cases represented a milder and less persistent form of the syndrome resembling systemic lupus erythematosus described by German authors.", "contents": "Pericarditis, pleural effusion, and pneumonitis with transient mitochondrial antibodies. Four women with fever, arthromyalgias, pericarditis, pleural effusion, high erythrocyte sedimentation rates, and lymphopenia had mitochondrial antibodies in the serum in the absence of antinuclear antibody. Their illness lasted 5-12 weeks and the antibody test results became negative on remission. Absence of specific bacteriological findings, normal antistreptolysin O titres, resistance to antibiotics, and good response to steroids suggested that these cases represented a milder and less persistent form of the syndrome resembling systemic lupus erythematosus described by German authors."}
{"id": "PMID:1078783", "title": "Hemophilus parainfluenzae meningitis.", "content": "Although Hemophilus influenzae is a common cause of meningitis, other members of the Hemophilus genus are rarely the infecting organism. Of 56 cases of meningitis due to Hemophilus species obseved at one hospital in the period 1970-74, 53 were due to H. influenzae and 3 to H. parainfluenzae. In the cases of H. parainfluenzae meningitis the clinical picture was complicated by associated sepsis, and therapy with ampicillin was not entirely satisfactory.", "contents": "Hemophilus parainfluenzae meningitis. Although Hemophilus influenzae is a common cause of meningitis, other members of the Hemophilus genus are rarely the infecting organism. Of 56 cases of meningitis due to Hemophilus species obseved at one hospital in the period 1970-74, 53 were due to H. influenzae and 3 to H. parainfluenzae. In the cases of H. parainfluenzae meningitis the clinical picture was complicated by associated sepsis, and therapy with ampicillin was not entirely satisfactory."}
{"id": "PMID:1078784", "title": "Suppression of secondary cellular immunity to a tumor allograft by cyclophosphamide and 1,3-bis(2-chloroethyl)-1-nitrosourea.", "content": "C57BL/6 mice (H-2b) were immunized with lethally x-irradiated Moloney virus-induced lymphoma cells of BALB/c origin (H-2d) on Days 0 and 10 and received rug on Days 11 and 14. Their spleen cells were then tested for reactivity against Moloney virus-induced lymphoma of BALB/c origin by the 51Cr-release cytotoxicity assay. In non-drug-treated mice the secondary cytotoxic response was maximal on Days 14 to 15, declined rapidley, and recurred after Day 21. The cytotoxic effector cells were shown to be theta-bearing T-lymphocytes. Cyclophosphamide (CY), 180 mg/kg, given on Day 11, totally prevented the development of a cytotoxic response and when given on Day 14 abolished the response already established. CY, 48 mg/kg, as well as 1,3-bis(2-chloroethyl)-1-nitrosourea 33 mg/kg, were almost as suppressive. Immune mice given CY on Day 14 and reimmunized on Day 36 exhibited a normal tertiary response. Mice similarly immunized on Days 1 and 10 and given drugs on Day 14 were challenged on Day 15 with up to 3.5 x 10-8 viable Moloney virus-induced lymphoma cells of BALB/c origin. Despite H-2 incompatibility, all nonimmune control mice developed ascites and died, whereas all mice immunized but not given drug failed to develop ascites. By contrast, 17 of 34 immunized mice given CY, 180 mg/kg, and 7 of 34 given 1,3-bis(2-chloroethyl)-1-nitrosourea developed ascites. The ascites eventually regressed. THE RESULTS SHOW THAT CY and 1,3-bis(2-chloroethyl)-1-nitrosourea can suppress a secondary cellular immune response as measured by the T-cell-mediated 51Cr-release cytotoxicity assay in vitro and by viable tumor challenge in vivo.", "contents": "Suppression of secondary cellular immunity to a tumor allograft by cyclophosphamide and 1,3-bis(2-chloroethyl)-1-nitrosourea. C57BL/6 mice (H-2b) were immunized with lethally x-irradiated Moloney virus-induced lymphoma cells of BALB/c origin (H-2d) on Days 0 and 10 and received rug on Days 11 and 14. Their spleen cells were then tested for reactivity against Moloney virus-induced lymphoma of BALB/c origin by the 51Cr-release cytotoxicity assay. In non-drug-treated mice the secondary cytotoxic response was maximal on Days 14 to 15, declined rapidley, and recurred after Day 21. The cytotoxic effector cells were shown to be theta-bearing T-lymphocytes. Cyclophosphamide (CY), 180 mg/kg, given on Day 11, totally prevented the development of a cytotoxic response and when given on Day 14 abolished the response already established. CY, 48 mg/kg, as well as 1,3-bis(2-chloroethyl)-1-nitrosourea 33 mg/kg, were almost as suppressive. Immune mice given CY on Day 14 and reimmunized on Day 36 exhibited a normal tertiary response. Mice similarly immunized on Days 1 and 10 and given drugs on Day 14 were challenged on Day 15 with up to 3.5 x 10-8 viable Moloney virus-induced lymphoma cells of BALB/c origin. Despite H-2 incompatibility, all nonimmune control mice developed ascites and died, whereas all mice immunized but not given drug failed to develop ascites. By contrast, 17 of 34 immunized mice given CY, 180 mg/kg, and 7 of 34 given 1,3-bis(2-chloroethyl)-1-nitrosourea developed ascites. The ascites eventually regressed. THE RESULTS SHOW THAT CY and 1,3-bis(2-chloroethyl)-1-nitrosourea can suppress a secondary cellular immune response as measured by the T-cell-mediated 51Cr-release cytotoxicity assay in vitro and by viable tumor challenge in vivo."}
{"id": "PMID:1078785", "title": "Metastasis formation of Yoshida sarcoma heterotransplanted in adult golden hamsters treated with anti-hamster thymocyte serum.", "content": "Growth of Yoshida sarcoma cells heterotransplanted in adult golden hamsters treated with antithymocyte serum was investigated. Control animals were grafted with or without hydrocoritisone conditioning. In the antithymocyte serum-treated group, tumors were produced in cheek pouches and grew progressively in size until Day 21 with no sign of regression. Little host reaction was noticed histologically around the tumor grafts throughout the course. Distant metastases were revealed at autopsy in 9 of 12 animals from Day 14 of inoculation on, and all the experimental animals died by Day 23. Six of the 9 hamsters with metastases died of tumor. By means of the back-transplantation test, the metastatic tumor cells were shown to be consistent with Yoshida sarcoma cells. In hydrocortisone-treated and untreated groups, the tumor size reached a maximum on Day 21 and Day 5, respectively, and then regression followed. No metastasis was detected in either control group.", "contents": "Metastasis formation of Yoshida sarcoma heterotransplanted in adult golden hamsters treated with anti-hamster thymocyte serum. Growth of Yoshida sarcoma cells heterotransplanted in adult golden hamsters treated with antithymocyte serum was investigated. Control animals were grafted with or without hydrocoritisone conditioning. In the antithymocyte serum-treated group, tumors were produced in cheek pouches and grew progressively in size until Day 21 with no sign of regression. Little host reaction was noticed histologically around the tumor grafts throughout the course. Distant metastases were revealed at autopsy in 9 of 12 animals from Day 14 of inoculation on, and all the experimental animals died by Day 23. Six of the 9 hamsters with metastases died of tumor. By means of the back-transplantation test, the metastatic tumor cells were shown to be consistent with Yoshida sarcoma cells. In hydrocortisone-treated and untreated groups, the tumor size reached a maximum on Day 21 and Day 5, respectively, and then regression followed. No metastasis was detected in either control group."}
{"id": "PMID:1078786", "title": "Malignant transformation of mouse cells by cigarette smoke condensate.", "content": "A low-nicotine cigarette smoke condensate, 12 fractions of the condensate, and a reconstituted sample were tested for their ability to induce transformation in the mouse cell line 3H/10T-1/2 CL-8. This cell line is noted for its remarkable low spontaneous rate of transformation. Both the crude condensate and the reconstituted sample as well as two specific fractions induced transformation in the mouse cells. These transformed cells produced fibrosarcomas when injected s.c. into antithymocyte serum-treated syngeneic mice.", "contents": "Malignant transformation of mouse cells by cigarette smoke condensate. A low-nicotine cigarette smoke condensate, 12 fractions of the condensate, and a reconstituted sample were tested for their ability to induce transformation in the mouse cell line 3H/10T-1/2 CL-8. This cell line is noted for its remarkable low spontaneous rate of transformation. Both the crude condensate and the reconstituted sample as well as two specific fractions induced transformation in the mouse cells. These transformed cells produced fibrosarcomas when injected s.c. into antithymocyte serum-treated syngeneic mice."}
{"id": "PMID:1078787", "title": "Changes in RNA in relation to growth of the fibroblast. III. Posttranscriptional regulation of mRNA formation in resting and growing cells.", "content": "The proportion of hnRNA processed into cytoplasmic mRNA in resting and growing 3T3 and 3T6 cells has been investigated by measuring the efficiency of transfer of nuclear poly(A) to the cytoplasm. When nuclear poly(A) is either labeled continuously with precursor or pulse labeled and then chased with cordycepin, growing cells transfer approximately twice as much of the poly(A) from the nucleus to the cytoplasm as do resting cells. When cells undergo a serum-induced transition from the resting to growing state, the efficiency of poly(A) transfer is increased to that characteristic of growing cells by 3 hr after the addition of the serum. On the other hand, the proportion of hnRNA which is polyadenylated and the total nuclear poly(A) content are the same in resting and growing cells. It appears that the principal means by which the cell regulates its poly(A)+ mRNA content in the growing and resting states is by regulating the efficiency which nuclear poly(A)+ RNA is converted to cytoplasmic mRNA.", "contents": "Changes in RNA in relation to growth of the fibroblast. III. Posttranscriptional regulation of mRNA formation in resting and growing cells. The proportion of hnRNA processed into cytoplasmic mRNA in resting and growing 3T3 and 3T6 cells has been investigated by measuring the efficiency of transfer of nuclear poly(A) to the cytoplasm. When nuclear poly(A) is either labeled continuously with precursor or pulse labeled and then chased with cordycepin, growing cells transfer approximately twice as much of the poly(A) from the nucleus to the cytoplasm as do resting cells. When cells undergo a serum-induced transition from the resting to growing state, the efficiency of poly(A) transfer is increased to that characteristic of growing cells by 3 hr after the addition of the serum. On the other hand, the proportion of hnRNA which is polyadenylated and the total nuclear poly(A) content are the same in resting and growing cells. It appears that the principal means by which the cell regulates its poly(A)+ mRNA content in the growing and resting states is by regulating the efficiency which nuclear poly(A)+ RNA is converted to cytoplasmic mRNA."}
{"id": "PMID:1078794", "title": "Effect of alterations of arterial blood pressure and heart rate on segmental dyskinesis during acute myocardial ischemia and following coronary reperfusion.", "content": "The effect of alterations of blood pressure and heart rate on segmental dyskinesis induced by coronary artery ligation and the influence of such manipulations on the magnitude of recovery of the syskinetic segment during subsequent coronary artery reperfusion were evaluated in 77 open-chest dogs. Wall motion was recorded by ultrasound reflected directly from the ischemic myocardial segment. Acute ischemia produced characteristic regional abnormalities in wall motion: aneurysmal bulging occurred during isometric contraction and wall velocity was markedly reduced during ventricular ejection. During 69 minutes of ischemia, a control group of dogs underwent no interventions and showed no further changes in wall motion. Tachycardia induced by atrial pacing during ischemia had no significant effect. Arterial hypertension during ischemia caused a marked reduction in wall velocity when methoxamine was used: 14 plus or minus 2 (SE) mm/sec (ischemia alone) to 6 plus or minus 1 mm/sec (ischemia + drug). In contrast, norepinephrine improved wall velocity: 11 plus or minus 2 mm/sec (ischemia alone) to 25 plus or minus 4 mm/sec (ischemia + drug). Hypertension caused by infusion of phenylephrine gave intermediate results, as did hypotension induced by either nitroprusside or hemorrhage during the ischemic period. After 60 minutes the drugs were stopped, the coronary ligation released, and the ischemic myocardium reperfused. The relative order of improvement of wall velocity with reperfusion was 11 plus or minus 2 mm/sec (ischemia alone) to 24 plus or minus 3 mm/sec (reperfusion) in the group that received norepinephrine, 12 plus or minus 3 mm/sec to 20 plus or minus 3 mm/sec in the control group with no intervention durin ischemia, 13 plus or minus 2 mm/sec to 20 plus or minus 1 mm/sec in the nitroprusside group, 9 plus or minus 2 mm/sec in the phenylephrine group, and 14 plus or minus 2 mm/sec to 12 plus or minus 1 mm/sec in the methoxamine group. The aneurysmal bulging during isometric contraction  also was reduced to a greater degree by reperfusion in the group that received norepinephrine during the ischemic period than it was in the groups undergoing other interventions during ischemia. We conclude that drug-induced elevations in arterial blood pressure can have different effects on the syskinetic motion of acutely ischemic myocardium and on the degree of recovery following reperfusion depending on the particular agent used.", "contents": "Effect of alterations of arterial blood pressure and heart rate on segmental dyskinesis during acute myocardial ischemia and following coronary reperfusion. The effect of alterations of blood pressure and heart rate on segmental dyskinesis induced by coronary artery ligation and the influence of such manipulations on the magnitude of recovery of the syskinetic segment during subsequent coronary artery reperfusion were evaluated in 77 open-chest dogs. Wall motion was recorded by ultrasound reflected directly from the ischemic myocardial segment. Acute ischemia produced characteristic regional abnormalities in wall motion: aneurysmal bulging occurred during isometric contraction and wall velocity was markedly reduced during ventricular ejection. During 69 minutes of ischemia, a control group of dogs underwent no interventions and showed no further changes in wall motion. Tachycardia induced by atrial pacing during ischemia had no significant effect. Arterial hypertension during ischemia caused a marked reduction in wall velocity when methoxamine was used: 14 plus or minus 2 (SE) mm/sec (ischemia alone) to 6 plus or minus 1 mm/sec (ischemia + drug). In contrast, norepinephrine improved wall velocity: 11 plus or minus 2 mm/sec (ischemia alone) to 25 plus or minus 4 mm/sec (ischemia + drug). Hypertension caused by infusion of phenylephrine gave intermediate results, as did hypotension induced by either nitroprusside or hemorrhage during the ischemic period. After 60 minutes the drugs were stopped, the coronary ligation released, and the ischemic myocardium reperfused. The relative order of improvement of wall velocity with reperfusion was 11 plus or minus 2 mm/sec (ischemia alone) to 24 plus or minus 3 mm/sec (reperfusion) in the group that received norepinephrine, 12 plus or minus 3 mm/sec to 20 plus or minus 3 mm/sec in the control group with no intervention durin ischemia, 13 plus or minus 2 mm/sec to 20 plus or minus 1 mm/sec in the nitroprusside group, 9 plus or minus 2 mm/sec in the phenylephrine group, and 14 plus or minus 2 mm/sec to 12 plus or minus 1 mm/sec in the methoxamine group. The aneurysmal bulging during isometric contraction  also was reduced to a greater degree by reperfusion in the group that received norepinephrine during the ischemic period than it was in the groups undergoing other interventions during ischemia. We conclude that drug-induced elevations in arterial blood pressure can have different effects on the syskinetic motion of acutely ischemic myocardium and on the degree of recovery following reperfusion depending on the particular agent used."}
{"id": "PMID:1078795", "title": "Demonstration that the atria, ventricles, and lungs each are responsible for a tonic inhibition of the vasomotorcenter in the dog.", "content": "To localize the areas of the cardiopulmonary region involved in tonic inhibition of the vasomotor center, anesthetized dogs were subjected to sinoaortic denervation and diaphragmatic vagotomy. Afferent vagal nerve traffic was interrupted in the neck by cooling. With the venous return taken from the venae cavae, oxygenated extracorporeally, and returned to the aorta, the heart was removed, leaving the ventilated lungs (condition 1), and the lungs and the ventricles were removed, leaving the beating atria (condition 3). With the venous return taken from the pulmonary arteries, oxygenated extracorporeally, and returned to the left atrium, the lungs were removed, leaving the intact working heart (condition 2), and the lungs were removed and the atria were denervated, leaving the working innervated ventricles (condition 4). Vagal cooling increased aortic pressure by 25 plus or minus 2 (SE) mm Hg in condition 1, by 36 plus or minus 2 mm Hg in condition 2, by 29 plus or minus 2 mm Hg in condition 3, and by 29 plus or minus 7 mm Hg in condition 4. Removing the atria in condition 3 or denervating the ventricles in condition 4 abolished the reflex response. Thus, afferent vagal nerves from the lungs and the heart tonically inhibit the vasomotor center. The inhibition exerted by the heart is caused by receptors in the atria and the ventricles.", "contents": "Demonstration that the atria, ventricles, and lungs each are responsible for a tonic inhibition of the vasomotorcenter in the dog. To localize the areas of the cardiopulmonary region involved in tonic inhibition of the vasomotor center, anesthetized dogs were subjected to sinoaortic denervation and diaphragmatic vagotomy. Afferent vagal nerve traffic was interrupted in the neck by cooling. With the venous return taken from the venae cavae, oxygenated extracorporeally, and returned to the aorta, the heart was removed, leaving the ventilated lungs (condition 1), and the lungs and the ventricles were removed, leaving the beating atria (condition 3). With the venous return taken from the pulmonary arteries, oxygenated extracorporeally, and returned to the left atrium, the lungs were removed, leaving the intact working heart (condition 2), and the lungs were removed and the atria were denervated, leaving the working innervated ventricles (condition 4). Vagal cooling increased aortic pressure by 25 plus or minus 2 (SE) mm Hg in condition 1, by 36 plus or minus 2 mm Hg in condition 2, by 29 plus or minus 2 mm Hg in condition 3, and by 29 plus or minus 7 mm Hg in condition 4. Removing the atria in condition 3 or denervating the ventricles in condition 4 abolished the reflex response. Thus, afferent vagal nerves from the lungs and the heart tonically inhibit the vasomotor center. The inhibition exerted by the heart is caused by receptors in the atria and the ventricles."}
{"id": "PMID:1078798", "title": "Chronic polymicrobial bacteremia.", "content": "A 13-year-old girl had chronic polymicrobial bacteremia with Enterobacter hafnia and Enterobacter agglomerans in the absence of any demonstrable underlying illness, use of immunosuppressive drugs, or discovery of portal of entry of the bacteria. She was treated successfully with a prolonged course of carbenicillin and aminoglycoside antibiotics which were tolerated well. The only side effect of the therapy was a transient episode of vestibular dysfunction which was reversible following cessation of gentamicin. The principles of management of polymicrobial bacteremia are presented.", "contents": "Chronic polymicrobial bacteremia. A 13-year-old girl had chronic polymicrobial bacteremia with Enterobacter hafnia and Enterobacter agglomerans in the absence of any demonstrable underlying illness, use of immunosuppressive drugs, or discovery of portal of entry of the bacteria. She was treated successfully with a prolonged course of carbenicillin and aminoglycoside antibiotics which were tolerated well. The only side effect of the therapy was a transient episode of vestibular dysfunction which was reversible following cessation of gentamicin. The principles of management of polymicrobial bacteremia are presented."}
{"id": "PMID:1078800", "title": "[Selective coronary perfusion scintigraphy. II. Abnormal coronary perfusion pattern (author's transl)].", "content": "Integrated assessment of selective coronary angiorgrams and perfusion scintigrams of 86 patients with coronary-artery insufficiency revealed anatomical and topographical details which could not be achieved with one of these methods alone. Pathological perfusion patterns were quite different from normal ones. The functional effects of single, centrally located stenoses, of more peripheral and multiple narrowings, as well as of anastomoses and collaterals were adequately demonstrated by selective coronary perfusion scintigraphy and prevented diagnostic errors. It is, therefore, an additional aid in the diagnosis of coronary-artery disease.", "contents": "[Selective coronary perfusion scintigraphy. II. Abnormal coronary perfusion pattern (author's transl)]. Integrated assessment of selective coronary angiorgrams and perfusion scintigrams of 86 patients with coronary-artery insufficiency revealed anatomical and topographical details which could not be achieved with one of these methods alone. Pathological perfusion patterns were quite different from normal ones. The functional effects of single, centrally located stenoses, of more peripheral and multiple narrowings, as well as of anastomoses and collaterals were adequately demonstrated by selective coronary perfusion scintigraphy and prevented diagnostic errors. It is, therefore, an additional aid in the diagnosis of coronary-artery disease."}
{"id": "PMID:1078803", "title": "Portacaval anastomosis and peptic ulcer: a nonassociation.", "content": "The incidence of peptic ulcer is increased in cirrhosis and is widely believed to be even greater in cirrhotic patients with portacaval anastomosis (PCA). Two prospective, controlled investigations of prophylactic PCA were evaluated to compare the frequency of peptic ulcer in two groups of cirrhotic patients with similar clinical and laboratory manifestations of cirrhosis randomly selected to be an unoperated Control Group (60 patients) or to have PCA (Shunt Group, 48 patients). In addition, nonrandomized groups of cirrhotic patients, 77 of whom were excluded from the randomized study and 44 of whom had therapeutic PCA, were studied. A diagnosis of chronic peptic ulcer was based on the demonstration of an ulcer crater by X-ray, endoscopy, surgery, or autopsy. Prior to inclusion in these studies, approximately 10% of patients had had peptic ulcer. After inclusion, during a mean follow-up period of 45 months, 12% of both the Control and Shunt Groups developed peptic ulcers. The frequency of complications of peptic ulcer, of recurrence of peptic ulcer, or of acute or symptomatic (unproved) ulcer were similar in both groups. Ulcers tended to develop later in shunted than in unshunted patients. Similar data were obtained from three of four other controlled investigations of PCA. This investigation does not find an increased occurrence of peptic ulcer after PCA. The frequency of ulcer in cirrhosis appears to increase with the duration of the disease independent of the presence or absence of PCA.", "contents": "Portacaval anastomosis and peptic ulcer: a nonassociation. The incidence of peptic ulcer is increased in cirrhosis and is widely believed to be even greater in cirrhotic patients with portacaval anastomosis (PCA). Two prospective, controlled investigations of prophylactic PCA were evaluated to compare the frequency of peptic ulcer in two groups of cirrhotic patients with similar clinical and laboratory manifestations of cirrhosis randomly selected to be an unoperated Control Group (60 patients) or to have PCA (Shunt Group, 48 patients). In addition, nonrandomized groups of cirrhotic patients, 77 of whom were excluded from the randomized study and 44 of whom had therapeutic PCA, were studied. A diagnosis of chronic peptic ulcer was based on the demonstration of an ulcer crater by X-ray, endoscopy, surgery, or autopsy. Prior to inclusion in these studies, approximately 10% of patients had had peptic ulcer. After inclusion, during a mean follow-up period of 45 months, 12% of both the Control and Shunt Groups developed peptic ulcers. The frequency of complications of peptic ulcer, of recurrence of peptic ulcer, or of acute or symptomatic (unproved) ulcer were similar in both groups. Ulcers tended to develop later in shunted than in unshunted patients. Similar data were obtained from three of four other controlled investigations of PCA. This investigation does not find an increased occurrence of peptic ulcer after PCA. The frequency of ulcer in cirrhosis appears to increase with the duration of the disease independent of the presence or absence of PCA."}
{"id": "PMID:1078804", "title": "Role of bile acids in the pathogenesis of aspirin-induced gastric mucosal hemorrhage in rats.", "content": "The simultaneous oral administration of acetylsalicylic acid (64 mg per kg) and taurocholic acid (2.5 mM) to rats induced gastric hemorrhagic mucosal bleeding in 82.4% of animals compared with 29.7% of animals given acetylsalicylic acid alone. Taurodeoxycholic acid and glycocholic acid with acetylsalicylic acid also significantly increased the incidence of gastric hemorrhagic mucosal bleeding compared with the control animals, but cholic acid, chenodeoxycholic acid, and deoxycholic acid did not have a significant effect. Hemorrhagic mucosal lesions were not observed when the bile acids studied were administered alone. These results indicate a possible role for certain bile acids in the pathogenesis of gastric mucosal bleeding after the ingestion of aspirin.", "contents": "Role of bile acids in the pathogenesis of aspirin-induced gastric mucosal hemorrhage in rats. The simultaneous oral administration of acetylsalicylic acid (64 mg per kg) and taurocholic acid (2.5 mM) to rats induced gastric hemorrhagic mucosal bleeding in 82.4% of animals compared with 29.7% of animals given acetylsalicylic acid alone. Taurodeoxycholic acid and glycocholic acid with acetylsalicylic acid also significantly increased the incidence of gastric hemorrhagic mucosal bleeding compared with the control animals, but cholic acid, chenodeoxycholic acid, and deoxycholic acid did not have a significant effect. Hemorrhagic mucosal lesions were not observed when the bile acids studied were administered alone. These results indicate a possible role for certain bile acids in the pathogenesis of gastric mucosal bleeding after the ingestion of aspirin."}
{"id": "PMID:1078805", "title": "Demonstration of superficial gastric erosions by double contrast radiography.", "content": "Superficial gastric erosions were found in 30 of 267 patients undergoing esophagogastroduodenoscopy. In 13 of these patients, and in 7 others not submitted to endoscopy, superficial gastric erosions were demonstrated radiologically by the double contrast technique. These may appear as flat, linear streaks, as dots of barium, or as target-like lesions with a central fleck of barium surrounded by a radiolucent halo. In 1 patient, a ring-shaped density was demonstrated due to a small blood clot adherent to a gastric erosion. Although patients presenting with acute hemorrhagic gastritis should still be examined first by endoscopy, double contrast radiography has been of value in patients presenting after the cessation of bleeding and in patients with vague digestive complaints. Because of our experience with the diagnosis of standard methods, and because of the Japanese experience with diagnosis of superficial gastric carcinoma, it is recommended that this technique be applied more widely to facilitate the detection of early and small lesions in the stomach.", "contents": "Demonstration of superficial gastric erosions by double contrast radiography. Superficial gastric erosions were found in 30 of 267 patients undergoing esophagogastroduodenoscopy. In 13 of these patients, and in 7 others not submitted to endoscopy, superficial gastric erosions were demonstrated radiologically by the double contrast technique. These may appear as flat, linear streaks, as dots of barium, or as target-like lesions with a central fleck of barium surrounded by a radiolucent halo. In 1 patient, a ring-shaped density was demonstrated due to a small blood clot adherent to a gastric erosion. Although patients presenting with acute hemorrhagic gastritis should still be examined first by endoscopy, double contrast radiography has been of value in patients presenting after the cessation of bleeding and in patients with vague digestive complaints. Because of our experience with the diagnosis of standard methods, and because of the Japanese experience with diagnosis of superficial gastric carcinoma, it is recommended that this technique be applied more widely to facilitate the detection of early and small lesions in the stomach."}
{"id": "PMID:1078812", "title": "Enhancement of reaginic antibody formation in the mouse by concanavalin A.", "content": "SWR mice injected with Concanavalin A (Con A) and Ovalbumin (Ov) one immediately after the other or with mixtures of Con A and Ov produced reaginic antibodies to Ov (titer 1:80) after primary immunization and reaginic antibody levels of 1:640 after secondary immunization. Mixtures of Con A and Ov were shown to contain soluble complexes which could be precipitated by 20% polyethylene glycol (PEG). Mixtures of Con A and human serum albumin or Con A and bovine serum albumin did not form soluble complexes and did not induce detectable reagin formation. The possible role of soluble of Con A-Ov complexes in stimulating reaginic antibody is discussed.", "contents": "Enhancement of reaginic antibody formation in the mouse by concanavalin A. SWR mice injected with Concanavalin A (Con A) and Ovalbumin (Ov) one immediately after the other or with mixtures of Con A and Ov produced reaginic antibodies to Ov (titer 1:80) after primary immunization and reaginic antibody levels of 1:640 after secondary immunization. Mixtures of Con A and Ov were shown to contain soluble complexes which could be precipitated by 20% polyethylene glycol (PEG). Mixtures of Con A and human serum albumin or Con A and bovine serum albumin did not form soluble complexes and did not induce detectable reagin formation. The possible role of soluble of Con A-Ov complexes in stimulating reaginic antibody is discussed."}
{"id": "PMID:1078815", "title": "Biological relevance of alkali-labile sites in double-stranded DNA after gamma-irradiation.", "content": "Alkali-labile sites produced by gamma-irradiation in dry RF-DNA of bacteriophage phi chi 174 were found to be lethal lesions with respect to the plaque-forming ability.", "contents": "Biological relevance of alkali-labile sites in double-stranded DNA after gamma-irradiation. Alkali-labile sites produced by gamma-irradiation in dry RF-DNA of bacteriophage phi chi 174 were found to be lethal lesions with respect to the plaque-forming ability."}
{"id": "PMID:1078816", "title": "Effects of gamma-radiation on the optical properties of aqueous lysozyme-polyinosinic-acid complexes.", "content": "The absorption of gamma-rays by dilute aqueous solutions of lysozyme and polyinosinic acid (poly I) causes significant increases in the light-scattering power of room temperature solutions in certain critical concentration regions. Light-scattering properties are unaltered by relatively large doses of radiation delivered to heated complexes. Prior irradiation of the poly I alone yields complexes whose light-scattering properties are the same as those of the unirradiated system. Studies of the radiation-induced absorbance changes of these complexes at 260 and 280 nm show behaviour indicative of the radiation chemistry of polynucleotide rather than of lysozyme. From the evidence available, it is postulated that most of the damage is to the poly I rather than lysozyme in the complexes. Increases in light-scattering and dissymmetry are attributed to unwinding of the multi-stranded polynucleotide. The role of water in the formation and radiation sensitivity of the complexes is discussed.", "contents": "Effects of gamma-radiation on the optical properties of aqueous lysozyme-polyinosinic-acid complexes. The absorption of gamma-rays by dilute aqueous solutions of lysozyme and polyinosinic acid (poly I) causes significant increases in the light-scattering power of room temperature solutions in certain critical concentration regions. Light-scattering properties are unaltered by relatively large doses of radiation delivered to heated complexes. Prior irradiation of the poly I alone yields complexes whose light-scattering properties are the same as those of the unirradiated system. Studies of the radiation-induced absorbance changes of these complexes at 260 and 280 nm show behaviour indicative of the radiation chemistry of polynucleotide rather than of lysozyme. From the evidence available, it is postulated that most of the damage is to the poly I rather than lysozyme in the complexes. Increases in light-scattering and dissymmetry are attributed to unwinding of the multi-stranded polynucleotide. The role of water in the formation and radiation sensitivity of the complexes is discussed."}
{"id": "PMID:1078817", "title": "Haemopoietic stem-cell kinetics during continuous irradiation.", "content": "Analysis of the kinetics of the biphasic response of haemopoietic stem cells to chronic low dose-rate irradiation indicates that a highly radiosensitive delay of entry into cell-cycle by Go cells may be responsible for the initial, apparently dose-rate-independent, phase of the dose-response curve. The second component of the curve appears to be the result of a combination of two factors: a relatively decreased differentiation demand on stem cells due to increased amplification in the transit-committed precursor cells, and a uniform shortening of the cell-cycle--including the S period--in the stem cells.", "contents": "Haemopoietic stem-cell kinetics during continuous irradiation. Analysis of the kinetics of the biphasic response of haemopoietic stem cells to chronic low dose-rate irradiation indicates that a highly radiosensitive delay of entry into cell-cycle by Go cells may be responsible for the initial, apparently dose-rate-independent, phase of the dose-response curve. The second component of the curve appears to be the result of a combination of two factors: a relatively decreased differentiation demand on stem cells due to increased amplification in the transit-committed precursor cells, and a uniform shortening of the cell-cycle--including the S period--in the stem cells."}
{"id": "PMID:1078818", "title": "DNA-to-protein cross-linking in synchronized HeLa cells exposed to ultra-violet light.", "content": "The U.V.-induced DNA-to-protein cross-linking in synchronously growing HeLa cells exhibits age-dependent variations. The maximum yield of cross-linking is observed during S-phase and exposure of mid-S cells to 250 ergs/mm2 renders about 22 per cent of DNA unextractable owing to cross-linking with proteins. Cells irradiated in G1 or G2 exhibit much smaller amount of cross-linking. The unextracted DNA was recovered from the protein fraction, and the addition of 0-5 per cent trypsin solution rendered all the DNA extractable. Fluctuations in the age-response pattern for the yield of DNA-protein cross-links varies throughout the cell-cycle in the same way as the efficiency for cell-killing, i.e. cells are resistant in G1, sensitive as they move into and through the S phase, and resistant again in G2. Dose-response curves determined at different times in the cycle reveal that up to about 500 ergs/mm2 the amount of DNA recovered from S cells decreased for about 8 per cent for every 100 ergs/mm2 of U.V. light. The amount of recovered DNA remained unchanged for cells exposed to 500 ergs/mm2 in the middle of S, for as long as 8 hours after exposure, i.e. for as long as the experiments were carried out no recovery from this damage was observed.", "contents": "DNA-to-protein cross-linking in synchronized HeLa cells exposed to ultra-violet light. The U.V.-induced DNA-to-protein cross-linking in synchronously growing HeLa cells exhibits age-dependent variations. The maximum yield of cross-linking is observed during S-phase and exposure of mid-S cells to 250 ergs/mm2 renders about 22 per cent of DNA unextractable owing to cross-linking with proteins. Cells irradiated in G1 or G2 exhibit much smaller amount of cross-linking. The unextracted DNA was recovered from the protein fraction, and the addition of 0-5 per cent trypsin solution rendered all the DNA extractable. Fluctuations in the age-response pattern for the yield of DNA-protein cross-links varies throughout the cell-cycle in the same way as the efficiency for cell-killing, i.e. cells are resistant in G1, sensitive as they move into and through the S phase, and resistant again in G2. Dose-response curves determined at different times in the cycle reveal that up to about 500 ergs/mm2 the amount of DNA recovered from S cells decreased for about 8 per cent for every 100 ergs/mm2 of U.V. light. The amount of recovered DNA remained unchanged for cells exposed to 500 ergs/mm2 in the middle of S, for as long as 8 hours after exposure, i.e. for as long as the experiments were carried out no recovery from this damage was observed."}
{"id": "PMID:1078819", "title": "A major component of the radiation effect: interference with endocellular control of cell proliferation and differentiation.", "content": "Cytological investigations with diploid and autotetraploid plant materials reveal that cytoplasmic growth, including the propagation of autoreduplicating cytoplasmic units such as mitochondria and proplastids, continues during the radiation-induced mitotic delay, thus increasing the ratio of cytoplasmic amount: nuclear ploidy. In accordance with a control mechanism, detected in the plant endosperm, this increase causes mitotic inhibition due to any early induction of otherwise normal differentiation processes. Although mutations are not a prerequisite, they can act in an auxiliary manner by quantitative action. The contribution of 'early differentiation' to non-survival varies according to irradiation conditions and can outweigh the direct effects of irreversible genetic damage. Therefore, a higher level of ploidy cannot improve survival significantly, if mitotically-active tissues are irradiated under the usual conditions. Corresponding observations are known from animals. The results suggest that the control mechanism of the endosperm is (a) general to multicellular organisms, and (b) can be modulated by extracellular agents.", "contents": "A major component of the radiation effect: interference with endocellular control of cell proliferation and differentiation. Cytological investigations with diploid and autotetraploid plant materials reveal that cytoplasmic growth, including the propagation of autoreduplicating cytoplasmic units such as mitochondria and proplastids, continues during the radiation-induced mitotic delay, thus increasing the ratio of cytoplasmic amount: nuclear ploidy. In accordance with a control mechanism, detected in the plant endosperm, this increase causes mitotic inhibition due to any early induction of otherwise normal differentiation processes. Although mutations are not a prerequisite, they can act in an auxiliary manner by quantitative action. The contribution of 'early differentiation' to non-survival varies according to irradiation conditions and can outweigh the direct effects of irreversible genetic damage. Therefore, a higher level of ploidy cannot improve survival significantly, if mitotically-active tissues are irradiated under the usual conditions. Corresponding observations are known from animals. The results suggest that the control mechanism of the endosperm is (a) general to multicellular organisms, and (b) can be modulated by extracellular agents."}
{"id": "PMID:1078820", "title": "Interaction of gamma-ray and neutron effects on the proliferative capacity of Chinese hamster cells.", "content": "The loss of reproductive capacity of Chinese hamster cells has been studied using 60Co gamma-rays and DT neutrons singly or combined in fixed proportions. Surviving fractions were measured down to 2 times 10-4. Interaction between the effects produced by neutrons and gamma-rays in the mixed irradiations has been demonstrated. The results are compared with those predicted by the cellular inactivation model developed by Katz and co-workers from their delta-ray theory of track structure.", "contents": "Interaction of gamma-ray and neutron effects on the proliferative capacity of Chinese hamster cells. The loss of reproductive capacity of Chinese hamster cells has been studied using 60Co gamma-rays and DT neutrons singly or combined in fixed proportions. Surviving fractions were measured down to 2 times 10-4. Interaction between the effects produced by neutrons and gamma-rays in the mixed irradiations has been demonstrated. The results are compared with those predicted by the cellular inactivation model developed by Katz and co-workers from their delta-ray theory of track structure."}
{"id": "PMID:1078822", "title": "Calcium-containing structures in vertebrate glial cells. Ultrastructural and microprobe analysis.", "content": "Electron probe microanalysis has revealed that vesicular or cisternal structures containing electron-dense material in frog ependymal glial cells contain deposits of calcium and phosphorus. The so-called \"osmiophilic particles\" in human astrocytes also contain calcium. It is suggested that these organelles are storage sites of calcium.", "contents": "Calcium-containing structures in vertebrate glial cells. Ultrastructural and microprobe analysis. Electron probe microanalysis has revealed that vesicular or cisternal structures containing electron-dense material in frog ependymal glial cells contain deposits of calcium and phosphorus. The so-called \"osmiophilic particles\" in human astrocytes also contain calcium. It is suggested that these organelles are storage sites of calcium."}
{"id": "PMID:1078823", "title": "Electrophoretic analysis of liver and testis histones of the frog Rana pipiens.", "content": "Histones were extracted from frog livers and testes and analyzed by electrophoresis on long polyacrylamide gels and on sodium dodecyl sulfate (SDS)-containing polyacrylamide gels. Frog histones were found to be similar to those of calf thymus except that frog histone fraction F2A2 showed a marked dependence on the temperature at which the long gels were run, and frog histone fraction F3 could be separated from frog F2B on SDS-containing gels. Comparisons between frog liver and frog testis histones indicated that the testis contains as its major F1 component a fast migrating species not found in liver. Testis histones also showed less microheterogeneity of fractions F3 and F2A1 than liver histones. These were the only differences observed between liver and testis histones, even when testis histones were prepared from sperm suspensions that were rich in cells in the late stages of spermiogenesis. Thus it seems that, in Rana, the electrophoretic properties of the basic proteins of sperm differ from those of somatic cells only in the nature of histone F1 and in the degree of microheterogeneity of fractions F2A1 and F3.", "contents": "Electrophoretic analysis of liver and testis histones of the frog Rana pipiens. Histones were extracted from frog livers and testes and analyzed by electrophoresis on long polyacrylamide gels and on sodium dodecyl sulfate (SDS)-containing polyacrylamide gels. Frog histones were found to be similar to those of calf thymus except that frog histone fraction F2A2 showed a marked dependence on the temperature at which the long gels were run, and frog histone fraction F3 could be separated from frog F2B on SDS-containing gels. Comparisons between frog liver and frog testis histones indicated that the testis contains as its major F1 component a fast migrating species not found in liver. Testis histones also showed less microheterogeneity of fractions F3 and F2A1 than liver histones. These were the only differences observed between liver and testis histones, even when testis histones were prepared from sperm suspensions that were rich in cells in the late stages of spermiogenesis. Thus it seems that, in Rana, the electrophoretic properties of the basic proteins of sperm differ from those of somatic cells only in the nature of histone F1 and in the degree of microheterogeneity of fractions F2A1 and F3."}
{"id": "PMID:1078824", "title": "Size and shape of transverse tubule openings in frog twitch muscle fibers.", "content": "The openings of transverse tubules in frog twitch fibers are described. The tubules open to the extracellular space by a narrow neck, with an inner diameter of 20 nm. The most peripheral portion of the tubules is tortuous and has a variable diameter. The similarity in size of the openings of T tubules and caveolae and the meandering path of the tubules are sufficient to account for the paucity of observed openings.", "contents": "Size and shape of transverse tubule openings in frog twitch muscle fibers. The openings of transverse tubules in frog twitch fibers are described. The tubules open to the extracellular space by a narrow neck, with an inner diameter of 20 nm. The most peripheral portion of the tubules is tortuous and has a variable diameter. The similarity in size of the openings of T tubules and caveolae and the meandering path of the tubules are sufficient to account for the paucity of observed openings."}
{"id": "PMID:1078825", "title": "Complement fixation by rheumatoid factor.", "content": "The capacity for fixation and activation of hemolytic complement by polyclonal IgM rheumatoid factors (RF) isolated from sera of patients with rheumatoid arthritis and monoclonal IgM-RF isolated from the cryoprecipitates of patients with IgM-IgG mixed cryoglobulinemia was examined. RF mixed with aggregated, reduced, and alkylated human IgG (Agg-R/A-IgG) in the fluid phase failed to significantly reduce the level of total hemolytic complement, CH50, or of individual complement components, C1, C2, C3, and C5. However, sheep erythrocytes (SRC) coated with Agg-R/A-IgG or with reduced and alkylated rabbit IgG anti-SRC antibody were hemolyzed by complement in the presence of polyclonal IgM-RF. Human and guinea pig complement worked equally well. The degree of hemolysis was in direct proportion to the hemagglutination titer of the RF against the same coated cells. Monoclonal IgM-RF, normal human IgM, and purified Waldenstr\u00f6m macroglobulins without antiglobulin activity were all inert. Hemolysis of coated SRC by RF and complement was inhibited by prior treatment of the complement source with chelating agents, hydrazine, cobra venom factor, specific antisera to C1q, CR, C5, C6, or C8, or by heating at 56 degrees C for 30 min. Purified radiolabeled C4, C3, and C8 included in the complement source were bound to hemolysed SRC in direct proportion to the degree of hemolysis. These data indicate that polyclonal IgM-RF fix and activate complement via the classic pathway. The system described for assessing complement fixation by isolated RF is readily adaptable to use with whole human serum.", "contents": "Complement fixation by rheumatoid factor. The capacity for fixation and activation of hemolytic complement by polyclonal IgM rheumatoid factors (RF) isolated from sera of patients with rheumatoid arthritis and monoclonal IgM-RF isolated from the cryoprecipitates of patients with IgM-IgG mixed cryoglobulinemia was examined. RF mixed with aggregated, reduced, and alkylated human IgG (Agg-R/A-IgG) in the fluid phase failed to significantly reduce the level of total hemolytic complement, CH50, or of individual complement components, C1, C2, C3, and C5. However, sheep erythrocytes (SRC) coated with Agg-R/A-IgG or with reduced and alkylated rabbit IgG anti-SRC antibody were hemolyzed by complement in the presence of polyclonal IgM-RF. Human and guinea pig complement worked equally well. The degree of hemolysis was in direct proportion to the hemagglutination titer of the RF against the same coated cells. Monoclonal IgM-RF, normal human IgM, and purified Waldenstr\u00f6m macroglobulins without antiglobulin activity were all inert. Hemolysis of coated SRC by RF and complement was inhibited by prior treatment of the complement source with chelating agents, hydrazine, cobra venom factor, specific antisera to C1q, CR, C5, C6, or C8, or by heating at 56 degrees C for 30 min. Purified radiolabeled C4, C3, and C8 included in the complement source were bound to hemolysed SRC in direct proportion to the degree of hemolysis. These data indicate that polyclonal IgM-RF fix and activate complement via the classic pathway. The system described for assessing complement fixation by isolated RF is readily adaptable to use with whole human serum."}
{"id": "PMID:1078826", "title": "Osteogenic sarcoma. Immunologic parameters before and during immunotherapy with tumor-specific transfer factor.", "content": "18 patients with osteogenic sarcoma were followed by serial measurements in vitro of tumor-specific cell-mediated cytotoxicity and of \"active\" and total rosette-forming T-cells. 13 of these patients have had or are currently receiving injections of osteogenic sarcoma-specific dialyzable transfer factor derived from healthy donors. In three patients with very small lesions, cytotoxicity was high before amputation and decreased within 2 mo after removal of tumor. Cytotoxicity was low at time of diagnosis in all patients with large tumor masses. The cytotoxicity of the patients' lymphocytes increased after administration of tumor-specific transfer factor in all patients so treated. Patients receiving nonspecific transfer factor showed evidence of declining cell-mediated cytotoxicity. Tumor-specific transfer factor may produce an increase in cell-mediated cytotoxicity to the tumor in patients with osteogenic sarcoma. This possibility is suggested by the pain and edema that occurred in the area of the tumor in patients who had metastatic disease when therapy was started and by lymphocytic infiltrates in the tumor, as well as by the increase in cell-mediated cytotoxicity and the increase in percentage of active rosette-forming cells from subnormal to normal. Serial measurements of cell-mediated cytotoxicity are helpful in monitoring the efficacy of transfer factor and other modes of therapy in these patients, and these measurements are the best available criteria for selection of donors of tumor-specific transfer factor.", "contents": "Osteogenic sarcoma. Immunologic parameters before and during immunotherapy with tumor-specific transfer factor. 18 patients with osteogenic sarcoma were followed by serial measurements in vitro of tumor-specific cell-mediated cytotoxicity and of \"active\" and total rosette-forming T-cells. 13 of these patients have had or are currently receiving injections of osteogenic sarcoma-specific dialyzable transfer factor derived from healthy donors. In three patients with very small lesions, cytotoxicity was high before amputation and decreased within 2 mo after removal of tumor. Cytotoxicity was low at time of diagnosis in all patients with large tumor masses. The cytotoxicity of the patients' lymphocytes increased after administration of tumor-specific transfer factor in all patients so treated. Patients receiving nonspecific transfer factor showed evidence of declining cell-mediated cytotoxicity. Tumor-specific transfer factor may produce an increase in cell-mediated cytotoxicity to the tumor in patients with osteogenic sarcoma. This possibility is suggested by the pain and edema that occurred in the area of the tumor in patients who had metastatic disease when therapy was started and by lymphocytic infiltrates in the tumor, as well as by the increase in cell-mediated cytotoxicity and the increase in percentage of active rosette-forming cells from subnormal to normal. Serial measurements of cell-mediated cytotoxicity are helpful in monitoring the efficacy of transfer factor and other modes of therapy in these patients, and these measurements are the best available criteria for selection of donors of tumor-specific transfer factor."}
{"id": "PMID:1078827", "title": "Tumor-specific cell-mediated immunity in household contacts of cancer patients..", "content": "Patients with osteogenic sarcoma (and related tumors), hypernephroma, and breast carcinoma, and their household contacts were tested for tumor-specific cell-mediated immunity against these tumors with the use of a short-term chromium-51 release assay. This assay, reproducible over many months and well-correlated with the clinical course of the patients, was used to demonstrate that household contacts of patients with osteogenic sarcoma and breast carcinoma have specific immunity against the tumor type with which they have been in contact. In both types of tumors, the range of cytotoxicity values produced by lymphocytes from the household contacts was significantly higher than that of the normal population. The incidence of immunity was much higher in household contacts of patients with breast carcinoma than in those of patients with osteogenic sarcoma. Immunity was found with equal frequency in men and women, as well as in genetically and nongenetically related household contacts (guardians, adopted children, spouses). Immunity against hypernephroma was not demonstrated in either patients with hypernephroma or their household contacts.", "contents": "Tumor-specific cell-mediated immunity in household contacts of cancer patients.. Patients with osteogenic sarcoma (and related tumors), hypernephroma, and breast carcinoma, and their household contacts were tested for tumor-specific cell-mediated immunity against these tumors with the use of a short-term chromium-51 release assay. This assay, reproducible over many months and well-correlated with the clinical course of the patients, was used to demonstrate that household contacts of patients with osteogenic sarcoma and breast carcinoma have specific immunity against the tumor type with which they have been in contact. In both types of tumors, the range of cytotoxicity values produced by lymphocytes from the household contacts was significantly higher than that of the normal population. The incidence of immunity was much higher in household contacts of patients with breast carcinoma than in those of patients with osteogenic sarcoma. Immunity was found with equal frequency in men and women, as well as in genetically and nongenetically related household contacts (guardians, adopted children, spouses). Immunity against hypernephroma was not demonstrated in either patients with hypernephroma or their household contacts."}
{"id": "PMID:1078828", "title": "A cytofluorometric and radiochemical analysis of the uptake and turnover of 5-hydroxytryptamine in mast cells.", "content": "Uptake and turnover of 5-hydroxytryptamine (5-HT) after the administration of 5-hydroxytryptophan (5-HTP) has been studied in rat peritoneal mast cells using radiochemical and quantitative cytofluorometric methods. There was a close agreement between the results obtained with the two methods. The extreme sensitivity of the cytofluorometric method was indicated by the fact that 0.2 pg 5-HT contained in mast cells from control rats could be readily quantitated. The cytofluorometric analysis demonstrated a large variation in 5-HT content of individual mast cells within the mast cell populations. The storage capacity for 5-HT greatly exceeded the amount found in normal mast cells. Intraperitoncally injected 5-HTP was rapidly taken up and eliminated from the mast cells within 12 hr, while 5-HT, probably derived from intracellular decarboxylation of 5-HTP, was retained in the cells and slowly eliminated. The elimination of 5-HT followed an exponential course and the half-life was found to be about 10 days. The results indicate that the turnover of 5-HT is much slower in mast cells than in other cells which normally store 5-HT.", "contents": "A cytofluorometric and radiochemical analysis of the uptake and turnover of 5-hydroxytryptamine in mast cells. Uptake and turnover of 5-hydroxytryptamine (5-HT) after the administration of 5-hydroxytryptophan (5-HTP) has been studied in rat peritoneal mast cells using radiochemical and quantitative cytofluorometric methods. There was a close agreement between the results obtained with the two methods. The extreme sensitivity of the cytofluorometric method was indicated by the fact that 0.2 pg 5-HT contained in mast cells from control rats could be readily quantitated. The cytofluorometric analysis demonstrated a large variation in 5-HT content of individual mast cells within the mast cell populations. The storage capacity for 5-HT greatly exceeded the amount found in normal mast cells. Intraperitoncally injected 5-HTP was rapidly taken up and eliminated from the mast cells within 12 hr, while 5-HT, probably derived from intracellular decarboxylation of 5-HTP, was retained in the cells and slowly eliminated. The elimination of 5-HT followed an exponential course and the half-life was found to be about 10 days. The results indicate that the turnover of 5-HT is much slower in mast cells than in other cells which normally store 5-HT."}
{"id": "PMID:1078829", "title": "Effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. II. Stimulation of the efferent arc.", "content": "The delayed-type hypersensitivity (DTH) produced by subcutaneous or intravenous immunization of mice with sheep red blood cells (SRBC) is mediated by T cells in cooperation with accessory cells (monocytes). Different effects of bacterial lipopolysaccharide (LPS) on this T cell activity were found at different stages of the immune response. a) T cell activity was enhanced by LPS given 1 to 3 days after sensitization. Enhancement, which was most marked when treatment was given on the 2nd day of the immune response, did not occur unless LPS was introduced into a site that drains to the responding lymph node. This stimulatory effect was dose dependent. b) A similar effect on the response to intravenous immunization caused DTH to appear transiently in mice given a T cell-blocking dose (10-9) of SRBC. c) DTH was suppressed by LPS given at the height of the T cell response (day 4). This effect, which was short lived and reversible, was due to the action of LPS on the accessor cells needed for the expression of DTH. Treatment of recipients with LPS completely blocked the adoptive transfer of DTH to both normal and spenectomized mice. The ability to react was regained slowly, but it returned promptly to splenectomized recipients. LPS has clearly more than one effect on the expression of cell-mediated immunity because it affects specific and non-specific mediator cells in different ways.", "contents": "Effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. II. Stimulation of the efferent arc. The delayed-type hypersensitivity (DTH) produced by subcutaneous or intravenous immunization of mice with sheep red blood cells (SRBC) is mediated by T cells in cooperation with accessory cells (monocytes). Different effects of bacterial lipopolysaccharide (LPS) on this T cell activity were found at different stages of the immune response. a) T cell activity was enhanced by LPS given 1 to 3 days after sensitization. Enhancement, which was most marked when treatment was given on the 2nd day of the immune response, did not occur unless LPS was introduced into a site that drains to the responding lymph node. This stimulatory effect was dose dependent. b) A similar effect on the response to intravenous immunization caused DTH to appear transiently in mice given a T cell-blocking dose (10-9) of SRBC. c) DTH was suppressed by LPS given at the height of the T cell response (day 4). This effect, which was short lived and reversible, was due to the action of LPS on the accessor cells needed for the expression of DTH. Treatment of recipients with LPS completely blocked the adoptive transfer of DTH to both normal and spenectomized mice. The ability to react was regained slowly, but it returned promptly to splenectomized recipients. LPS has clearly more than one effect on the expression of cell-mediated immunity because it affects specific and non-specific mediator cells in different ways."}
{"id": "PMID:1078830", "title": "A lectin model for unresponsiveness. I. Reversible effects of high doses of concanavalin A on spontaneous and induced DNA synthesis.", "content": "A mitogen model for unresponsiveness has been studied. In 72-hr cultures the lowest supraoptimal dose of Concanavalin (Con A) showing no stimulation of rat thymocytes above background was 160 mug/ml. This dose was also found to inhibit substantially (50%) the high spontaneous DNA synthesis by thymocytes during the first 24 hr of culture. This effect was most significant 12 to 24 hr after addition of high-dose Con A. It was not necessary for the cells to remain continuously with high-dose Con A, since washing out unbound Con A after 1 hr still led to reduced 3-H-thymidine incorporation at 24 or 72 hr. Furthermore, high-dose Con A treatment suppressed DNA synthesis when added at 48 hr to cultured optimally stimulated with Con A. Interaction with high-dose Con A appears to inhibit the ability of cells to initiate new rounds of DNA synthesis without blocking ongoing DNA replication. Removal of Con A with alpha-methyl-D-mannopyranoside (mannose) led to only partial reversal of these effects after 1 hr although \"unresponsiveness\" was reversible at 15 min. Suppression was not due to cytotoxic or agglutinating effects of the high-dose Con A. Two times 10-6 thymocytes exposed to 160 mug of Con A for 1 hr bound 1.7 mug, 86% of which was elutable with mannose. There was no reduction in the ability of high-dose treated cells to bind a subsequent optimal dose of Con A. Since Con A binds to essentially all thymocytes, this system is useful in following early events in the induction of lectin \"unresponsiveness\" as a model for immunologic tolerance.", "contents": "A lectin model for unresponsiveness. I. Reversible effects of high doses of concanavalin A on spontaneous and induced DNA synthesis. A mitogen model for unresponsiveness has been studied. In 72-hr cultures the lowest supraoptimal dose of Concanavalin (Con A) showing no stimulation of rat thymocytes above background was 160 mug/ml. This dose was also found to inhibit substantially (50%) the high spontaneous DNA synthesis by thymocytes during the first 24 hr of culture. This effect was most significant 12 to 24 hr after addition of high-dose Con A. It was not necessary for the cells to remain continuously with high-dose Con A, since washing out unbound Con A after 1 hr still led to reduced 3-H-thymidine incorporation at 24 or 72 hr. Furthermore, high-dose Con A treatment suppressed DNA synthesis when added at 48 hr to cultured optimally stimulated with Con A. Interaction with high-dose Con A appears to inhibit the ability of cells to initiate new rounds of DNA synthesis without blocking ongoing DNA replication. Removal of Con A with alpha-methyl-D-mannopyranoside (mannose) led to only partial reversal of these effects after 1 hr although \"unresponsiveness\" was reversible at 15 min. Suppression was not due to cytotoxic or agglutinating effects of the high-dose Con A. Two times 10-6 thymocytes exposed to 160 mug of Con A for 1 hr bound 1.7 mug, 86% of which was elutable with mannose. There was no reduction in the ability of high-dose treated cells to bind a subsequent optimal dose of Con A. Since Con A binds to essentially all thymocytes, this system is useful in following early events in the induction of lectin \"unresponsiveness\" as a model for immunologic tolerance."}
{"id": "PMID:1078831", "title": "Characterization of immunoglobulin-bearing and other small lymphocytes in mouse bone marrow by sedimentation and electrophoresis.", "content": "125I-antiglobulin binding and radioautography have been used to define the sedimentation and electrophoretic properties of immunoglobulin-bearing and other small lymphocytes in bone marrow. Bone marrow cell suspensions from CBA mice were exposed to 125I-labeled rabbit anti-mouse immunoglobulin for 30 min at 0 degrees C. Cell fractions were collected after either sedimentation at unit gravity or continuous free-buffer film preparative electrophoresis. The sedimentation profiles of labeled, antiglobulin-binding small lymphocytes and of unlabeled small lymphocytes were identical, peaking at 2.6 mm/hr, except for 5% of the labeled small lymphocytes which sedimented at 3.8 to 4.1 mm/hr. In electrophoretic fractions most labeled small lymphocytes were contained in a peak of low mobility and unlabeled small lymphocytes were of intermediate mobility, but high mobility fractions also contained small numbers of labeled and unlabeled small lymphocytes.  Small lymphocytes comprised 80 to 85% of all nucleated bone marrow cells in fractions taken at the peak of the small lymphocyte distribution profile after either sedimentation or electrophoresis, accompanied mainly by either erythroid or granulocytic precursor cells, respectively. The results demonstrate that small lymphocytes with readily detectable surface immunoglobulin in the bone marrow closely resemble those in peripheral lymphoid tissues with respect to their sedimentation and electrophoretic properties. The possibility is raised that the rapidly sedimenting immunoglobulin-bearing small lymphocytes in bone marrow are a functionally distinct group of potential precursor cells. Non-antiglobulin-binding small lymphocytes in bone marrow resemble electrophoretically the double negative (immunoglobulin -and theta -negative) small lymphocytes in other lymphoid tissues. Small lymphocytes of high electrophoretic mobility, prominent in the theta- bearing lymphocyte populations of peripheral lymphoid tissues, are few in bone marrow. Lymphocyte-rich suspensions, containing either high or low proportions of immunoglobulin-bearing small lymphocytes, can be separated from bone marrow by electrophoresis", "contents": "Characterization of immunoglobulin-bearing and other small lymphocytes in mouse bone marrow by sedimentation and electrophoresis. 125I-antiglobulin binding and radioautography have been used to define the sedimentation and electrophoretic properties of immunoglobulin-bearing and other small lymphocytes in bone marrow. Bone marrow cell suspensions from CBA mice were exposed to 125I-labeled rabbit anti-mouse immunoglobulin for 30 min at 0 degrees C. Cell fractions were collected after either sedimentation at unit gravity or continuous free-buffer film preparative electrophoresis. The sedimentation profiles of labeled, antiglobulin-binding small lymphocytes and of unlabeled small lymphocytes were identical, peaking at 2.6 mm/hr, except for 5% of the labeled small lymphocytes which sedimented at 3.8 to 4.1 mm/hr. In electrophoretic fractions most labeled small lymphocytes were contained in a peak of low mobility and unlabeled small lymphocytes were of intermediate mobility, but high mobility fractions also contained small numbers of labeled and unlabeled small lymphocytes.  Small lymphocytes comprised 80 to 85% of all nucleated bone marrow cells in fractions taken at the peak of the small lymphocyte distribution profile after either sedimentation or electrophoresis, accompanied mainly by either erythroid or granulocytic precursor cells, respectively. The results demonstrate that small lymphocytes with readily detectable surface immunoglobulin in the bone marrow closely resemble those in peripheral lymphoid tissues with respect to their sedimentation and electrophoretic properties. The possibility is raised that the rapidly sedimenting immunoglobulin-bearing small lymphocytes in bone marrow are a functionally distinct group of potential precursor cells. Non-antiglobulin-binding small lymphocytes in bone marrow resemble electrophoretically the double negative (immunoglobulin -and theta -negative) small lymphocytes in other lymphoid tissues. Small lymphocytes of high electrophoretic mobility, prominent in the theta- bearing lymphocyte populations of peripheral lymphoid tissues, are few in bone marrow. Lymphocyte-rich suspensions, containing either high or low proportions of immunoglobulin-bearing small lymphocytes, can be separated from bone marrow by electrophoresis"}
{"id": "PMID:1078832", "title": "Antibody-mediated suppression of grafted lymphoma. III. Evaluation of the role of thymic function, non-thymus-derived lymphocytes, macrophages, platelets, and polymorphonuclear leukocytes in syngeneic and allogeneic hosts.", "content": "Syngeneic or allogeneic mice pretreated with sublethal whole-body irradiation were rendered incapable of suppressing the growth of grafted tumor cells sensitized with alloantibody. The growth of sensitized tumor cells was suppressed when they were mixed with donor effector cells from mice syngeneic or allogeneic to the recipients and then were inoculated in irradiated recipients. Three donor-host combinations were used to study the suppression of the murine lymphoma 6C3HED indigenous to C3H mice. These were C3H donor cells in C3H recipients, C57BL/6 donor cells in C3H recipients, or C57BL/6 donor cells in C57BL/6 recipients. In all three combinations, macrophages obtained from an inflammatory exudate, exudate lymphocytes not bearing theta antigen, and platelets were, in descending order of effectiveness, consistently active in restoring antibody-mediated suppression of tumor growth in irradiated hosts. Prior irradiation of the transferred lymphocytes somewhat diminished their effectiveness. Freeze-thawed or heat-killed macrophages (but not freeze-thawed platelets or lymphocytes) were effective in restoration. Peripheral blood mononuclear leukocytes and splenic lymphoid cells were not active in the recipients syngeneic to the donor cells but were acitve in recipients allogeneic to the donor cells. Polymorphonuclear leukocytes isolated from peripheral blood or an inflammatory exudate were not active. Intact thymic function seems unimportant since antibody-mediated suppression took place as effectively in thymectomized mice as in normal controls.", "contents": "Antibody-mediated suppression of grafted lymphoma. III. Evaluation of the role of thymic function, non-thymus-derived lymphocytes, macrophages, platelets, and polymorphonuclear leukocytes in syngeneic and allogeneic hosts. Syngeneic or allogeneic mice pretreated with sublethal whole-body irradiation were rendered incapable of suppressing the growth of grafted tumor cells sensitized with alloantibody. The growth of sensitized tumor cells was suppressed when they were mixed with donor effector cells from mice syngeneic or allogeneic to the recipients and then were inoculated in irradiated recipients. Three donor-host combinations were used to study the suppression of the murine lymphoma 6C3HED indigenous to C3H mice. These were C3H donor cells in C3H recipients, C57BL/6 donor cells in C3H recipients, or C57BL/6 donor cells in C57BL/6 recipients. In all three combinations, macrophages obtained from an inflammatory exudate, exudate lymphocytes not bearing theta antigen, and platelets were, in descending order of effectiveness, consistently active in restoring antibody-mediated suppression of tumor growth in irradiated hosts. Prior irradiation of the transferred lymphocytes somewhat diminished their effectiveness. Freeze-thawed or heat-killed macrophages (but not freeze-thawed platelets or lymphocytes) were effective in restoration. Peripheral blood mononuclear leukocytes and splenic lymphoid cells were not active in the recipients syngeneic to the donor cells but were acitve in recipients allogeneic to the donor cells. Polymorphonuclear leukocytes isolated from peripheral blood or an inflammatory exudate were not active. Intact thymic function seems unimportant since antibody-mediated suppression took place as effectively in thymectomized mice as in normal controls."}
{"id": "PMID:1078833", "title": "The frequency of phosphorylcholine-specific B cells in conventional and germfree BALB/C mice.", "content": "The frequency of B cells specific for phosphorylcholine (PC) in spleens of nonimmune conventionally-reared and germfree adult Balb/c mice was determined by an in vitro splenic focus technique. Analysis of spleen cells from 11 conventionally reared donors revealed an average of 19.1 PC-specific clonal precursor cells/10-6 donor B cells when calculated utilizing a cloning efficiency previously determined for the in vitro splenic focus technique of 4%. This number is one-tenth the frequency determined for 2, 4-dinitrophenyl- and 2,4,6-trintrophenyl-specific B cells; however, both idiotypic and isoelectric analysis reveal that the majority of B cells specific for PC yield monoclonal antibodies which share the TEPC 15 idiotype and contain a homogeneous IgM peak with a pK of 4.60. Thus this single clone appears on the average to be represented by more than 10-3 B cells in conventionally reared nonimmune adults. A similar frequency for precursor cells of this clonotype was found in an analysis of seven  germfree adult mice so that the high frequency of B cells of this colontype appears to be independent of previous antigenic stimulation. Among the 11 conventionally reared donors analyzed, frequencies varied from 1.6 to 52/10-6 B cells--an extreme degree of variation when compared to the almost invariant frequencies observed for 2, 4-dinitrophenyl, 2, 4, 6-trinitrophenyl, and fluoroscein. This variation is interpreted as a reflection of the life cycle of a single clone with a possible role for antigen implied by smaller variations observed in grem free mice.", "contents": "The frequency of phosphorylcholine-specific B cells in conventional and germfree BALB/C mice. The frequency of B cells specific for phosphorylcholine (PC) in spleens of nonimmune conventionally-reared and germfree adult Balb/c mice was determined by an in vitro splenic focus technique. Analysis of spleen cells from 11 conventionally reared donors revealed an average of 19.1 PC-specific clonal precursor cells/10-6 donor B cells when calculated utilizing a cloning efficiency previously determined for the in vitro splenic focus technique of 4%. This number is one-tenth the frequency determined for 2, 4-dinitrophenyl- and 2,4,6-trintrophenyl-specific B cells; however, both idiotypic and isoelectric analysis reveal that the majority of B cells specific for PC yield monoclonal antibodies which share the TEPC 15 idiotype and contain a homogeneous IgM peak with a pK of 4.60. Thus this single clone appears on the average to be represented by more than 10-3 B cells in conventionally reared nonimmune adults. A similar frequency for precursor cells of this clonotype was found in an analysis of seven  germfree adult mice so that the high frequency of B cells of this colontype appears to be independent of previous antigenic stimulation. Among the 11 conventionally reared donors analyzed, frequencies varied from 1.6 to 52/10-6 B cells--an extreme degree of variation when compared to the almost invariant frequencies observed for 2, 4-dinitrophenyl, 2, 4, 6-trinitrophenyl, and fluoroscein. This variation is interpreted as a reflection of the life cycle of a single clone with a possible role for antigen implied by smaller variations observed in grem free mice."}
{"id": "PMID:1078834", "title": "Cellular cooperation during in vivo anti-hapten antibody responses. I. The effect of cell number on the response.", "content": "Cellular interactions in adoptive secondary anti-hapten antibody responses to the hapten 2, 4-dinitrophenyl (DNP) have been studied. It was shown that DNP-specific B cells must interact with carrier specific helper T cells to give optimal responses. Independent titration of B cell and helper cell activity in adoptive anti-DNP antibody responses gave the following results: Doubling the number of transferred B cells approximately doubled the subsequent antibody response. Doubling the number of helper cells leads to nearly 4 times as much anti-DNP antibody, measured 7 days after boosting (\"premium effect\"). This marked effect of helper cell number on the antibody response is thought to be due primarly to the interaction of two populations of carrier-specific cells in the helper effect, or to the interaction of two activities of a single population of helper cells, namely clone activation and clone expansion. Only a very small proportion of the premium effect given by helper cells could be attributed to increases in antibody affinity.", "contents": "Cellular cooperation during in vivo anti-hapten antibody responses. I. The effect of cell number on the response. Cellular interactions in adoptive secondary anti-hapten antibody responses to the hapten 2, 4-dinitrophenyl (DNP) have been studied. It was shown that DNP-specific B cells must interact with carrier specific helper T cells to give optimal responses. Independent titration of B cell and helper cell activity in adoptive anti-DNP antibody responses gave the following results: Doubling the number of transferred B cells approximately doubled the subsequent antibody response. Doubling the number of helper cells leads to nearly 4 times as much anti-DNP antibody, measured 7 days after boosting (\"premium effect\"). This marked effect of helper cell number on the antibody response is thought to be due primarly to the interaction of two populations of carrier-specific cells in the helper effect, or to the interaction of two activities of a single population of helper cells, namely clone activation and clone expansion. Only a very small proportion of the premium effect given by helper cells could be attributed to increases in antibody affinity."}
{"id": "PMID:1078835", "title": "Cellular cooperation during in vivo anti-hapten antibody responses. II. The effect of in vivo and in vitro x-irradiation on T and B cells.", "content": "The effect of x-irradiation on T and B cells involved in an in vivo adoptive anti-hapten antibody response has been studied. In vitro x-irradiation inactivates both B cells and T cells. B cells are somewhat more sensitive than T cells to such treatment, the dose required to reduce B cell activity by 50% being about 50 R, whereas 200 R will reduce helper cell activity by 50%. By contrast, if recipient mice are primed with carrier, they develop helper activity that is totally resistant to 500 R x-irradiation within 3 days of immunization. This activity can not be transferred from irradiated, carrier-primed mice by either spleen cells or serum, although anti-carrier antibody is shown to give a modest helper effect late after boosting. However, this radioresistant helper effect can be reproduced in a cell transfer system by giving carrier-primed spleen cells to normal recipients that are then x-irradiated (500R) and given a large dose of carrier protein. Neither cells nor carrier alone will give rise to such radioresistant helper effects. Similar helper activity is occasionally observed when normal mice are irradiated 24 hr after being given helper cells, in the abscence of carrier and boosted shortly thereafter. The differences between these radioresistant helper effects, and the radiosensitivity of helper cells transferred after x-irradiation, may be related to x-ray induced changes in cell migration patterns.", "contents": "Cellular cooperation during in vivo anti-hapten antibody responses. II. The effect of in vivo and in vitro x-irradiation on T and B cells. The effect of x-irradiation on T and B cells involved in an in vivo adoptive anti-hapten antibody response has been studied. In vitro x-irradiation inactivates both B cells and T cells. B cells are somewhat more sensitive than T cells to such treatment, the dose required to reduce B cell activity by 50% being about 50 R, whereas 200 R will reduce helper cell activity by 50%. By contrast, if recipient mice are primed with carrier, they develop helper activity that is totally resistant to 500 R x-irradiation within 3 days of immunization. This activity can not be transferred from irradiated, carrier-primed mice by either spleen cells or serum, although anti-carrier antibody is shown to give a modest helper effect late after boosting. However, this radioresistant helper effect can be reproduced in a cell transfer system by giving carrier-primed spleen cells to normal recipients that are then x-irradiated (500R) and given a large dose of carrier protein. Neither cells nor carrier alone will give rise to such radioresistant helper effects. Similar helper activity is occasionally observed when normal mice are irradiated 24 hr after being given helper cells, in the abscence of carrier and boosted shortly thereafter. The differences between these radioresistant helper effects, and the radiosensitivity of helper cells transferred after x-irradiation, may be related to x-ray induced changes in cell migration patterns."}
{"id": "PMID:1078836", "title": "Cellular cooperation during in vivo anti-hapten antibody responses. III. The helper cell activity of activated thymocytes, of spleen cells treated with anti-theta serum, and of spleen cells from anti-thymocyte serum-treated or adult thymectomized donors.", "content": "An adoptive secondary anti-2,4-dinitrophenyl (DNP) antibody response involving T-B cell collaboration has been studied. In particular, attempts have been made to affect the unexpectedly steep log dose-response curve obtained when graded numbers of helper cells are transferred to irradiated recipients given a fixed number of B cells (premium effect). A variety of means were used to alter helper cell activity, and this activity was then measured quantitatively, as was the ability of the helper cells present after these treatments to give a premium effect. It was shown that activated T cells are approximately twice as active as spleen cells in helper activity and give a comparable premium effect. Graded doses of anti-theta serum plus complement markedly reduce the helper activity of spleen cells without affecting the premium effect given by the residual cells. Treatment of primed cell donors with limited doses of heterologous anti-mouse thymocyte serum (ATS) before transfer does not affect B cell activity, but readily inactivates helper cells, again without affecting the premium effect given by the residual cells. Adult thymectomy (ATx) of helper cell donors before priming with carrier led initially to increased helper activity relative to age-matched control donors. This increase may reflect the loss of nonspecific suppressor T cells from spleens shortly after ATx. Late after ATx, there was about a 2-fold decrease in helper activity, probably reflecting a loss of helper cell precursors. At no time was there any change in the premium effect. In view of the failure of any of the techniques used to abolish the premium effect given by helper cells in this response, it seems likely that this premium effect is due to the cooperative interaction of two very similar types of mature T cell. Alternatively, the premium effect observed here may result from the interaction of two activities of a single type of T cell which is mediated by independent factors.", "contents": "Cellular cooperation during in vivo anti-hapten antibody responses. III. The helper cell activity of activated thymocytes, of spleen cells treated with anti-theta serum, and of spleen cells from anti-thymocyte serum-treated or adult thymectomized donors. An adoptive secondary anti-2,4-dinitrophenyl (DNP) antibody response involving T-B cell collaboration has been studied. In particular, attempts have been made to affect the unexpectedly steep log dose-response curve obtained when graded numbers of helper cells are transferred to irradiated recipients given a fixed number of B cells (premium effect). A variety of means were used to alter helper cell activity, and this activity was then measured quantitatively, as was the ability of the helper cells present after these treatments to give a premium effect. It was shown that activated T cells are approximately twice as active as spleen cells in helper activity and give a comparable premium effect. Graded doses of anti-theta serum plus complement markedly reduce the helper activity of spleen cells without affecting the premium effect given by the residual cells. Treatment of primed cell donors with limited doses of heterologous anti-mouse thymocyte serum (ATS) before transfer does not affect B cell activity, but readily inactivates helper cells, again without affecting the premium effect given by the residual cells. Adult thymectomy (ATx) of helper cell donors before priming with carrier led initially to increased helper activity relative to age-matched control donors. This increase may reflect the loss of nonspecific suppressor T cells from spleens shortly after ATx. Late after ATx, there was about a 2-fold decrease in helper activity, probably reflecting a loss of helper cell precursors. At no time was there any change in the premium effect. In view of the failure of any of the techniques used to abolish the premium effect given by helper cells in this response, it seems likely that this premium effect is due to the cooperative interaction of two very similar types of mature T cell. Alternatively, the premium effect observed here may result from the interaction of two activities of a single type of T cell which is mediated by independent factors."}
{"id": "PMID:1078837", "title": "Antibodies to thymocytes in sera of normal controls and of patients with schizophrenia.", "content": "The sera from normal healthy individuals and from patients with schizophrenia were tested for cytotoxic antibodies against thymic antigens located on thymocytes. It was found that the sera from schizophrenics do not show higher cytotoxicity to mouse and rabbit thymocytes than the sera from normal controls. Furthermore, absorption of sera with adult mouse thymocytes and brain failed to show any difference between the sera from these two groups of subjects.", "contents": "Antibodies to thymocytes in sera of normal controls and of patients with schizophrenia. The sera from normal healthy individuals and from patients with schizophrenia were tested for cytotoxic antibodies against thymic antigens located on thymocytes. It was found that the sera from schizophrenics do not show higher cytotoxicity to mouse and rabbit thymocytes than the sera from normal controls. Furthermore, absorption of sera with adult mouse thymocytes and brain failed to show any difference between the sera from these two groups of subjects."}
{"id": "PMID:1078838", "title": "Ontogeny of mouse lymphocyte function. II. Development of the ability to produce antibody is modulated by T lymphocytes.", "content": "The relative functional maturity of neonatal mouse spleen T- and B-cell populations was assessed by comparing the ability to respond to the thymic-independent antigen, DNP-Ficoll, or thymic-dependent SRBC by producing antibody in vitro. Although mouse spleen cells responded to DNP-Ficoll at an earlier age than they responded to SRBC or TNP-SRBC, the reason for the lag in the T-dependent response was confounded by the finding of high numbers of suppressor T lymphocytes in the neonatal spleen. Thus, small numbers of neonatal spleen T cells or thymocytes significantly decreased the in vitro antibody response of adult spleen cells. Although B lymphocytes appear to be functionally mature soon after birth, their acitivity may be modulated by an excess of suppressor T cells; e.g., the reconstitution of helper cell function in the neonatal spleen required anti-theta treatment before addition of adult helper cells. Suppressive activity attributable to T cells seems to play a dominant role in determining the ability of the neonatal animal to react positively or negatively to antigenic stimulation.", "contents": "Ontogeny of mouse lymphocyte function. II. Development of the ability to produce antibody is modulated by T lymphocytes. The relative functional maturity of neonatal mouse spleen T- and B-cell populations was assessed by comparing the ability to respond to the thymic-independent antigen, DNP-Ficoll, or thymic-dependent SRBC by producing antibody in vitro. Although mouse spleen cells responded to DNP-Ficoll at an earlier age than they responded to SRBC or TNP-SRBC, the reason for the lag in the T-dependent response was confounded by the finding of high numbers of suppressor T lymphocytes in the neonatal spleen. Thus, small numbers of neonatal spleen T cells or thymocytes significantly decreased the in vitro antibody response of adult spleen cells. Although B lymphocytes appear to be functionally mature soon after birth, their acitivity may be modulated by an excess of suppressor T cells; e.g., the reconstitution of helper cell function in the neonatal spleen required anti-theta treatment before addition of adult helper cells. Suppressive activity attributable to T cells seems to play a dominant role in determining the ability of the neonatal animal to react positively or negatively to antigenic stimulation."}
{"id": "PMID:1078839", "title": "Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells.", "content": "The cell-mediated cytotoxicity (CMC) of nonadherent cells from the peritoneal cavity (NAPC) of alloimmunized mice can be measured by the [3H]proline microassay. The exhibition of thymus-derived (T) cell antigens on these killer cells was studied by incubating them with the relevant T-cell antisera and complement (C), under optimal conditions for lysis, before performance of the CMC assay. Under these conditions, the following T-cell antigens were demonstrable on the killer population in terms of percent reduction in CMC by the respective antisera: (a) Thy-1.1 (83%) and Thy-1.2 (100%), (b) MSLA (86%), (c) NTA-RA (a T-cell antigen recognized by naturally occurring autoantibody of NZB mice) (62%), (d) Ly1.1 )58%, (e) Ly-2.1 (11%; considered a marginal result) and Ly-2.2 (63%), and (f) Ly-3.2 (77%). The following were not demonstrable: (g) TL, and (h) Ly-1.2. (i) The antigen Ly-3.1 was not studied. Omission of C deprived all T-cell antisera tested of their capacity to suppress CMC, indicating that the cell components recognized by such antisera may perform no direct function in CMC. On the assumption that all Ly+ cells are Thy-1+, it is clear that the T-cell members of the immune NAPC population must be heterogenous. This follows from the fact that the proportions of T cells lysed by different Ly antisera did not correspond with ensuing degree of loss of CMC capacity. The extremes were represented by anti-Ly-1.2 (74% Thy-1+ cells lysed, but no reduction in CMC) and Ly-3.2 (54% Thy-1+ cells lysed, with 77% reduction in CMC). From this initial survey it appears that the C57BL/6 mice killer T-cell population active in CMC in vitro is relatively rich in surface antigens of the  Ly-2/Ly-3 category and relatively poor in representation of the Ly-1 surface antigens. It remains to be seen whether this killer cell phenotype, poor in Ly-1 and rich in Ly-2/Ly-3, is characteristic of the mouse generally. From these results it appears that subsets of T cells with different immunological functions may exhibit qualitative or quantitative differences in surface antigens specified by different Ly loci; this will be easier to assess in the future when the results of experiments with the same Ly antisera but dealing with T-cell functions other than CMC become available.", "contents": "Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells. The cell-mediated cytotoxicity (CMC) of nonadherent cells from the peritoneal cavity (NAPC) of alloimmunized mice can be measured by the [3H]proline microassay. The exhibition of thymus-derived (T) cell antigens on these killer cells was studied by incubating them with the relevant T-cell antisera and complement (C), under optimal conditions for lysis, before performance of the CMC assay. Under these conditions, the following T-cell antigens were demonstrable on the killer population in terms of percent reduction in CMC by the respective antisera: (a) Thy-1.1 (83%) and Thy-1.2 (100%), (b) MSLA (86%), (c) NTA-RA (a T-cell antigen recognized by naturally occurring autoantibody of NZB mice) (62%), (d) Ly1.1 )58%, (e) Ly-2.1 (11%; considered a marginal result) and Ly-2.2 (63%), and (f) Ly-3.2 (77%). The following were not demonstrable: (g) TL, and (h) Ly-1.2. (i) The antigen Ly-3.1 was not studied. Omission of C deprived all T-cell antisera tested of their capacity to suppress CMC, indicating that the cell components recognized by such antisera may perform no direct function in CMC. On the assumption that all Ly+ cells are Thy-1+, it is clear that the T-cell members of the immune NAPC population must be heterogenous. This follows from the fact that the proportions of T cells lysed by different Ly antisera did not correspond with ensuing degree of loss of CMC capacity. The extremes were represented by anti-Ly-1.2 (74% Thy-1+ cells lysed, but no reduction in CMC) and Ly-3.2 (54% Thy-1+ cells lysed, with 77% reduction in CMC). From this initial survey it appears that the C57BL/6 mice killer T-cell population active in CMC in vitro is relatively rich in surface antigens of the  Ly-2/Ly-3 category and relatively poor in representation of the Ly-1 surface antigens. It remains to be seen whether this killer cell phenotype, poor in Ly-1 and rich in Ly-2/Ly-3, is characteristic of the mouse generally. From these results it appears that subsets of T cells with different immunological functions may exhibit qualitative or quantitative differences in surface antigens specified by different Ly loci; this will be easier to assess in the future when the results of experiments with the same Ly antisera but dealing with T-cell functions other than CMC become available."}
{"id": "PMID:1078840", "title": "Genetic regulation of the antibody response to H-2Db alloantigens in mice. I. Differences in activation of helper T cells in C57BL/10 and BALB/c congenic strains.", "content": "B10.A(5R) mice immunized with C57BL/10 spleen cells demonstrate a normal T-cell-mediated cytotoxicity to H-2Db tumor cells but they do not mount any IgG antibody response to H-2Db alloantigens. B10.A(5R) mice do show a high titered IgG response when immunized with A.BY cells, which differ at H-2Db plus non-H-2 cell surface antigens, or with B10.A(2R) cells, which differ at H-2Db, H-2Kk, and H-2Ik cell surface antigens. These findings indicate a failure of the T-helper cells to induce the switch from IgM to IgG when the H-2Db alloantigens are the only difference on the immunizing cell. In immunizing H-2d mice with congenic H-g2 cells which differ only in the H-2Db region, mice of the C57BL/10 background made only IgM antibodies whereas mice of the BALB/c background made IgG antibodies. This comparison confirms that genes separate from H-2 regulate the T-cell helper function. The genes that influence the T-cell helper function do not regulate the T-cell-mediated cytotoxicity.", "contents": "Genetic regulation of the antibody response to H-2Db alloantigens in mice. I. Differences in activation of helper T cells in C57BL/10 and BALB/c congenic strains. B10.A(5R) mice immunized with C57BL/10 spleen cells demonstrate a normal T-cell-mediated cytotoxicity to H-2Db tumor cells but they do not mount any IgG antibody response to H-2Db alloantigens. B10.A(5R) mice do show a high titered IgG response when immunized with A.BY cells, which differ at H-2Db plus non-H-2 cell surface antigens, or with B10.A(2R) cells, which differ at H-2Db, H-2Kk, and H-2Ik cell surface antigens. These findings indicate a failure of the T-helper cells to induce the switch from IgM to IgG when the H-2Db alloantigens are the only difference on the immunizing cell. In immunizing H-2d mice with congenic H-g2 cells which differ only in the H-2Db region, mice of the C57BL/10 background made only IgM antibodies whereas mice of the BALB/c background made IgG antibodies. This comparison confirms that genes separate from H-2 regulate the T-cell helper function. The genes that influence the T-cell helper function do not regulate the T-cell-mediated cytotoxicity."}
{"id": "PMID:1078841", "title": "Rosette-forming ability of thymus-derived lymphocytes in cell-mediated immunity. I. Delayed hypersensitivity and in vitro cytotoxicity.", "content": "Effector cells in delayed hypersensitivity and in vitro cytotoxicity were studied in lymph node cells from animals immunized with sheep erythrocytes (SRBC) in complete Freund's adjuvant. Delayed hypersensitivity response (DHR) was assayed by the increase in foot pad swelling after the intrafoot pad injection of immune cells plus antigen. Cell-mediated cytotoxicity against SRBC was assayed by a microcytotoxicity test with sheep fibroblasts as target cells. Effector cells were antigen specific, sensitive to anti-theta serum plus complement (C), and insensitive to anti-Ig serum plus C. A nonrosette-forming (non-RFC) small lymphocyte effector T cell and a rosette-forming medium lymphocyte effector T cell were isolated by velocity sedimentation. The small lymphocyte non-RFC required a longer time than the medium lymphocyte RFC effector cell to produce maximum activity. Buoyant density failed to distinguish medium lymphocyte effector cells in DHR and in vitro cytotoxicity.", "contents": "Rosette-forming ability of thymus-derived lymphocytes in cell-mediated immunity. I. Delayed hypersensitivity and in vitro cytotoxicity. Effector cells in delayed hypersensitivity and in vitro cytotoxicity were studied in lymph node cells from animals immunized with sheep erythrocytes (SRBC) in complete Freund's adjuvant. Delayed hypersensitivity response (DHR) was assayed by the increase in foot pad swelling after the intrafoot pad injection of immune cells plus antigen. Cell-mediated cytotoxicity against SRBC was assayed by a microcytotoxicity test with sheep fibroblasts as target cells. Effector cells were antigen specific, sensitive to anti-theta serum plus complement (C), and insensitive to anti-Ig serum plus C. A nonrosette-forming (non-RFC) small lymphocyte effector T cell and a rosette-forming medium lymphocyte effector T cell were isolated by velocity sedimentation. The small lymphocyte non-RFC required a longer time than the medium lymphocyte RFC effector cell to produce maximum activity. Buoyant density failed to distinguish medium lymphocyte effector cells in DHR and in vitro cytotoxicity."}
{"id": "PMID:1078842", "title": "Mechanical control of the time-course of contraction of the frog heart.", "content": "Changes in load during most phases of an isotonic contraction of the frog and turtle heart increased or decreased the duration of the twitch. It was abbreviated by a maintained increase or by a brief decrease in load. The relaxing effect of these procedures developed with a delay lasting more than a second under some conditions and will be called lengthening deactivation. The reverse procedures, a maintained diminution or a brief increase in load, increased the duration of the twitch. This effect will be called shortening activation. Although the termination of relaxation may be delayed or advanced by the mechanical interventions mentioned, the normal time-course of isotonic relaxation was always resumed later, regardless of the starting level of the load, making it possible to measure accurately changes in the duration of the twitch. The responses to changes in load produce positive feedback during the isotonic contraction and explain, at least in part, the difference in the time-course of isotonic and isometric contraction. The effects of changes in load were much smaller and briefer in the atrium than the ventricle.", "contents": "Mechanical control of the time-course of contraction of the frog heart. Changes in load during most phases of an isotonic contraction of the frog and turtle heart increased or decreased the duration of the twitch. It was abbreviated by a maintained increase or by a brief decrease in load. The relaxing effect of these procedures developed with a delay lasting more than a second under some conditions and will be called lengthening deactivation. The reverse procedures, a maintained diminution or a brief increase in load, increased the duration of the twitch. This effect will be called shortening activation. Although the termination of relaxation may be delayed or advanced by the mechanical interventions mentioned, the normal time-course of isotonic relaxation was always resumed later, regardless of the starting level of the load, making it possible to measure accurately changes in the duration of the twitch. The responses to changes in load produce positive feedback during the isotonic contraction and explain, at least in part, the difference in the time-course of isotonic and isometric contraction. The effects of changes in load were much smaller and briefer in the atrium than the ventricle."}
{"id": "PMID:1078843", "title": "Absent vestibulo-ocular reflexes and acute supratentorial lesions.", "content": "Loss of vestibulo-ocular reflexes occurred in two patients with acute supratentorial lesions who received therapeutic doses of anticonvulsant drugs. There was no clinical or angiographic evidence of focal brain-stem damage. Absence of vestibulo-ocular reflexes is attributed to a combination of acute cerebral damage and anticonvulsant drugs. The loss of these reflexes in patients with acute cerebral lesions cannot be interpreted as evidence of irreversible brain-stem injury.", "contents": "Absent vestibulo-ocular reflexes and acute supratentorial lesions. Loss of vestibulo-ocular reflexes occurred in two patients with acute supratentorial lesions who received therapeutic doses of anticonvulsant drugs. There was no clinical or angiographic evidence of focal brain-stem damage. Absence of vestibulo-ocular reflexes is attributed to a combination of acute cerebral damage and anticonvulsant drugs. The loss of these reflexes in patients with acute cerebral lesions cannot be interpreted as evidence of irreversible brain-stem injury."}
{"id": "PMID:1078844", "title": "Excitability modulation by taurine. Transmembrane measurements of neuromuscular transmission.", "content": "The presence of taurine, a non-essential amino acid, in nerve and muscle has been previously associated with inhibition of activity in the central nervous system, with the etiology of epileptogenic foci, and with the muscle weakness of muscular dystrophy. We present here data showing a small and probably insignificant effect of taurine on neuromuscular transmission per se, but significant hyperpolarization of the membrane potential in both taurine-incubated and taurine-loaded muscles. In addition, we found that taurine reduces the time course of the muscle action potential. The results are interpreted in terms of neuromuscular transmission and excitation-contraction coupling consequent to these phenomena. This interpretation is compatable with the hypothesis that taurine is involved in the genesis of muscular dystrophy where the membrane potential is depolarized. Our results and interpretation can also explain the anti-arrhythmic action of taurine on cardiac muscle.", "contents": "Excitability modulation by taurine. Transmembrane measurements of neuromuscular transmission. The presence of taurine, a non-essential amino acid, in nerve and muscle has been previously associated with inhibition of activity in the central nervous system, with the etiology of epileptogenic foci, and with the muscle weakness of muscular dystrophy. We present here data showing a small and probably insignificant effect of taurine on neuromuscular transmission per se, but significant hyperpolarization of the membrane potential in both taurine-incubated and taurine-loaded muscles. In addition, we found that taurine reduces the time course of the muscle action potential. The results are interpreted in terms of neuromuscular transmission and excitation-contraction coupling consequent to these phenomena. This interpretation is compatable with the hypothesis that taurine is involved in the genesis of muscular dystrophy where the membrane potential is depolarized. Our results and interpretation can also explain the anti-arrhythmic action of taurine on cardiac muscle."}
{"id": "PMID:1078857", "title": "Olfactory bulb projections in the bullfrog Rana catesbeiana.", "content": "The projections of the accessory and main olfactory bulbs of the bullfrog are described as part of a long term analysis of the morphological differences in amphibian and reptilian telancephalons. Unilateral aspiration of the accessory olfactory bulb results in an ipsilateral projection to the pars lateralis of the amygdala via the accessory olfactory tract. Degenerating fibers from the accessory olfactory bulb are tracable into the cell-free zone between the dorsal striatum and the lateral pallium, and projections to these neural populations may also exist. Unilateral lesions of the main olfactory bulb reveal two major secondary pathways: an ipsilateral medial olfactory tract that projects to the rostral ventromedial portion of the medial pallium, the postolfactory eminence and the rostal, lateral and medial septal nuclei; and an ipsilateral lateral olfactory tract that projects to the dorsal striatum, the lateral pallium and the ventral half of the dorsal pallium. Two crossed secondary olfactory pathways to the contralateral telencephalon decussate via the habenular commissure after entering the ipsilateral stria medullaris. A crossed lateral pathway terminates in the dorsal striatum, the caudal, lateral pallium and the ventral portion of the dorsal pallium. A crossed medial pathway terminates in the internal granule layer of the main olfactory bulb.", "contents": "Olfactory bulb projections in the bullfrog Rana catesbeiana. The projections of the accessory and main olfactory bulbs of the bullfrog are described as part of a long term analysis of the morphological differences in amphibian and reptilian telancephalons. Unilateral aspiration of the accessory olfactory bulb results in an ipsilateral projection to the pars lateralis of the amygdala via the accessory olfactory tract. Degenerating fibers from the accessory olfactory bulb are tracable into the cell-free zone between the dorsal striatum and the lateral pallium, and projections to these neural populations may also exist. Unilateral lesions of the main olfactory bulb reveal two major secondary pathways: an ipsilateral medial olfactory tract that projects to the rostral ventromedial portion of the medial pallium, the postolfactory eminence and the rostal, lateral and medial septal nuclei; and an ipsilateral lateral olfactory tract that projects to the dorsal striatum, the lateral pallium and the ventral half of the dorsal pallium. Two crossed secondary olfactory pathways to the contralateral telencephalon decussate via the habenular commissure after entering the ipsilateral stria medullaris. A crossed lateral pathway terminates in the dorsal striatum, the caudal, lateral pallium and the ventral portion of the dorsal pallium. A crossed medial pathway terminates in the internal granule layer of the main olfactory bulb."}
{"id": "PMID:1078863", "title": "[Binocular inferior altitudinal hemianopia following occult hemorrhage (author's transl)].", "content": "Authors report a case of binocular inferior altitudinal hemianopia caused by occult haemorrhage from the gastrointestinal tract. There was a 36 hours' period between the onset of visual field defects of the two eyes. The ocular signs have thrown light on the grave anaemic condition. The internal state of the patient was rapidly improving but the visual field defects have remained the same.", "contents": "[Binocular inferior altitudinal hemianopia following occult hemorrhage (author's transl)]. Authors report a case of binocular inferior altitudinal hemianopia caused by occult haemorrhage from the gastrointestinal tract. There was a 36 hours' period between the onset of visual field defects of the two eyes. The ocular signs have thrown light on the grave anaemic condition. The internal state of the patient was rapidly improving but the visual field defects have remained the same."}
{"id": "PMID:1078864", "title": "[The border lines between the 3 forms of trichromaticity in anomaloscopic examinations (author's transl)].", "content": "After presentation of the up to date knowledge on transition form between normal and protanomalous trichromates as well as the gap between normal and deuteranomalous trichromates and author reports on 20,000 investigations of his own on colour vision. Registration of all special cases since 1957 are presented. With reference to the large material it is found that, when all important factors of this investigation are condidred, there is a relatively sharp border between normal and the deuteranomalous at an Ag of 1.75, whereas in the transitional zone of Ag 0.4-0.85 there is marked overlapping between protanomalous and normal trichromates especially in the region of Ag 0.6-0.8. Whereas a differentiation between normal and the deuteranomalous is easily made through the Ag further methods, especially observation of the position of the yellow serew of the anomaloscope. Are always required to differentiate between normal and protanomalous trichromates.", "contents": "[The border lines between the 3 forms of trichromaticity in anomaloscopic examinations (author's transl)]. After presentation of the up to date knowledge on transition form between normal and protanomalous trichromates as well as the gap between normal and deuteranomalous trichromates and author reports on 20,000 investigations of his own on colour vision. Registration of all special cases since 1957 are presented. With reference to the large material it is found that, when all important factors of this investigation are condidred, there is a relatively sharp border between normal and the deuteranomalous at an Ag of 1.75, whereas in the transitional zone of Ag 0.4-0.85 there is marked overlapping between protanomalous and normal trichromates especially in the region of Ag 0.6-0.8. Whereas a differentiation between normal and the deuteranomalous is easily made through the Ag further methods, especially observation of the position of the yellow serew of the anomaloscope. Are always required to differentiate between normal and protanomalous trichromates."}
{"id": "PMID:1078866", "title": "Revascularization of the stenotic left main coronary artery and impaired left ventricle.", "content": "The high mortality rate associated with revascularization for stenosis of the left main coronary artery and impairment of the left ventricle (classes III and IV) has been significantly reduced by a twofold approach: combating hypotension during induction of anesthesia and preventing ischemia resulting from anoxic arrest, often needed to facilitate the insertion of the left coronary anastomoses. These two goals have been successfully achieved by (1) a readiness to institute circulatory assist by means of femoral-to-femoral cardiopulmonary bypass and (2) augmentation of coronary flow through immediate insertion of a vein graft between the aorta and right coronary artery, if the anatomy permits.", "contents": "Revascularization of the stenotic left main coronary artery and impaired left ventricle. The high mortality rate associated with revascularization for stenosis of the left main coronary artery and impairment of the left ventricle (classes III and IV) has been significantly reduced by a twofold approach: combating hypotension during induction of anesthesia and preventing ischemia resulting from anoxic arrest, often needed to facilitate the insertion of the left coronary anastomoses. These two goals have been successfully achieved by (1) a readiness to institute circulatory assist by means of femoral-to-femoral cardiopulmonary bypass and (2) augmentation of coronary flow through immediate insertion of a vein graft between the aorta and right coronary artery, if the anatomy permits."}
{"id": "PMID:1078867", "title": "Multiple myeloma. A clinical review.", "content": "This group of proliferative diseases of the plasma cell line, in which manifestations of abnormal immunoglobulin production are associated with variable degrees of depressed antibody synthesis, pose fascinating problems crossing many clinical and research disciplines. The present state of diagnosis and clinical management is assessed.", "contents": "Multiple myeloma. A clinical review. This group of proliferative diseases of the plasma cell line, in which manifestations of abnormal immunoglobulin production are associated with variable degrees of depressed antibody synthesis, pose fascinating problems crossing many clinical and research disciplines. The present state of diagnosis and clinical management is assessed."}
{"id": "PMID:1078884", "title": "In vivo interaction of the Kunitz protease inhibitor and of insulin with subcellular structures from rat renal cortex.", "content": "Rats were injected with labeled Kunitz protease inhibitor and killed at various times thereafter.  Radioactivity was measured in various fractions of kidney homogenates in order to study the time-dependent fixation to different cell organelles, expecially the transition from the brush border to lysosome fraction.  With short survival periods (up to 5 min), the renal protease inhibitor is recovered nearly completely with the brush border fraction.  With longer periods, a shift towards particles with higher densities and higher beta-glucuronidase activities takes place.  Similar results have been achieved with insulin.  Lysosomes were prepared and subfractionated following i. v. administration of the protease inhibitor or insulin.  The radioactivity of the peptides was found in the lysosomal range of density.  According to our present and previous results, the renal pathway of the protease inhibitor consists of 3 steps: binding to the brush border, reabsorption into micropinocytotic vesicles and phagosomes, and final enrichment in phagolysosomes with subsequent degradation.  We suggest this type of transport to be representative for peptides in general.", "contents": "In vivo interaction of the Kunitz protease inhibitor and of insulin with subcellular structures from rat renal cortex. Rats were injected with labeled Kunitz protease inhibitor and killed at various times thereafter.  Radioactivity was measured in various fractions of kidney homogenates in order to study the time-dependent fixation to different cell organelles, expecially the transition from the brush border to lysosome fraction.  With short survival periods (up to 5 min), the renal protease inhibitor is recovered nearly completely with the brush border fraction.  With longer periods, a shift towards particles with higher densities and higher beta-glucuronidase activities takes place.  Similar results have been achieved with insulin.  Lysosomes were prepared and subfractionated following i. v. administration of the protease inhibitor or insulin.  The radioactivity of the peptides was found in the lysosomal range of density.  According to our present and previous results, the renal pathway of the protease inhibitor consists of 3 steps: binding to the brush border, reabsorption into micropinocytotic vesicles and phagosomes, and final enrichment in phagolysosomes with subsequent degradation.  We suggest this type of transport to be representative for peptides in general."}
{"id": "PMID:1078885", "title": "Thromboembolism in pregnancy.", "content": "Eighteen patients were given carefully controlled intravenous heparin for thromboembolic disease of pregnancy. Thirteen were treated in later gestation and postparium, mainly following warfin therapy. One was treated in early pregnancy and 4 in the postpartum period. Heparin was delivered by constant infusion and dosage regulated by maintaining the thrombin time at 2 to/ times normal. Therapy was stopped for a few hours before and after delivery so that the thrombin time was normal or nearly normal at birth. Two minor episodes of thrombosis recurred in 1 patient while on warfarin, and significant postpartum bleeding occurred in one other. Substituting heparin for warfarin in late gestation was of value in preventing neonatal bleeding complications.", "contents": "Thromboembolism in pregnancy. Eighteen patients were given carefully controlled intravenous heparin for thromboembolic disease of pregnancy. Thirteen were treated in later gestation and postparium, mainly following warfin therapy. One was treated in early pregnancy and 4 in the postpartum period. Heparin was delivered by constant infusion and dosage regulated by maintaining the thrombin time at 2 to/ times normal. Therapy was stopped for a few hours before and after delivery so that the thrombin time was normal or nearly normal at birth. Two minor episodes of thrombosis recurred in 1 patient while on warfarin, and significant postpartum bleeding occurred in one other. Substituting heparin for warfarin in late gestation was of value in preventing neonatal bleeding complications."}
{"id": "PMID:1078892", "title": "Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.", "content": "A polypeptide of 8500 molecular weight is described that induces the differentiation of T (thymus-derived) cell and B (bone-marrow-derived) cell immunocytes in vitro, apparently via beta-adrenergic receptors and adenylate cyclase activation. This polypeptide shows a high degree of evolutionary conservation, exhibiting close structural, functional, and immunological similarity when isolated from such diverse origins as cells of mammals and higher plants. This polypeptide was detected in animal cells, yeast, bacteria, and higher plants, and so may well be a universal constituent of living cells.", "contents": "Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells. A polypeptide of 8500 molecular weight is described that induces the differentiation of T (thymus-derived) cell and B (bone-marrow-derived) cell immunocytes in vitro, apparently via beta-adrenergic receptors and adenylate cyclase activation. This polypeptide shows a high degree of evolutionary conservation, exhibiting close structural, functional, and immunological similarity when isolated from such diverse origins as cells of mammals and higher plants. This polypeptide was detected in animal cells, yeast, bacteria, and higher plants, and so may well be a universal constituent of living cells."}
{"id": "PMID:1078893", "title": "The immunobiology of skin cancer.", "content": "The immunobiology of skin cancer was studied with thymus-dependent lymphocyte (T cell) levels (an in vitro measure of cellular immunity), with lymphocytic infiltration (LI) of the tumor (an in vivo measure of host-tumor relationship), and with HL-A typing (a genetic measure of histocompatibility). The T cell levels in preoperative patients with squamous (SCC) and basal (BCC) cell carcinoma were significantly lower than in the non-cancer control population (normals). The T cell levels were significantly lower in patients with large tumors than in those with small tumors. The T cell levels remained significantly low in patients cured of large tumors, but were normal in those cured of small tumors. Patients with Bowen's disease not only had T cell levels significantly lower than normal (as a group), but there was also a significant increase in the number of patients who had T cell levels less than two standard deviations below the normal mean. This may signify that they have a greater risk of developing a second kind of malignancy elsewhere. There was a direct correlation between the degree of lymphocytic infiltration (LI) of the tumor, the tumor size, and the T cell level. Small, well-localized tumors had a marked LI and high T cell levels--while the large, deeply invasive tumors had a minimal, or absent, LI and low T cell levels. The presence of HL-A antigens 1 and 8 correlated both with a tendency toward large tumors and with low T cell levels. This may represent the association of a human immune response gene with the human histocompatibility locus. Possibilities for the application of these findings in the clinical management of skin cancer are discussed.", "contents": "The immunobiology of skin cancer. The immunobiology of skin cancer was studied with thymus-dependent lymphocyte (T cell) levels (an in vitro measure of cellular immunity), with lymphocytic infiltration (LI) of the tumor (an in vivo measure of host-tumor relationship), and with HL-A typing (a genetic measure of histocompatibility). The T cell levels in preoperative patients with squamous (SCC) and basal (BCC) cell carcinoma were significantly lower than in the non-cancer control population (normals). The T cell levels were significantly lower in patients with large tumors than in those with small tumors. The T cell levels remained significantly low in patients cured of large tumors, but were normal in those cured of small tumors. Patients with Bowen's disease not only had T cell levels significantly lower than normal (as a group), but there was also a significant increase in the number of patients who had T cell levels less than two standard deviations below the normal mean. This may signify that they have a greater risk of developing a second kind of malignancy elsewhere. There was a direct correlation between the degree of lymphocytic infiltration (LI) of the tumor, the tumor size, and the T cell level. Small, well-localized tumors had a marked LI and high T cell levels--while the large, deeply invasive tumors had a minimal, or absent, LI and low T cell levels. The presence of HL-A antigens 1 and 8 correlated both with a tendency toward large tumors and with low T cell levels. This may represent the association of a human immune response gene with the human histocompatibility locus. Possibilities for the application of these findings in the clinical management of skin cancer are discussed."}
{"id": "PMID:1078895", "title": "Agenesis of the corpus callosum with interhemispheric cyst.", "content": "The radiographic features of a cerebral malformation are presented. The major findings are absence of the corpus callosum, a large interhemispheric cyst, and ventricular dilatation. This cerebral dysplasia originates in the first trimester of fetal development resulting in gross psychomotor deficiencies and seizure disorders. Diagnosis which depends predominantly on encephalographic or ventriculographic findings enables formulation of a guarded prognosis.", "contents": "Agenesis of the corpus callosum with interhemispheric cyst. The radiographic features of a cerebral malformation are presented. The major findings are absence of the corpus callosum, a large interhemispheric cyst, and ventricular dilatation. This cerebral dysplasia originates in the first trimester of fetal development resulting in gross psychomotor deficiencies and seizure disorders. Diagnosis which depends predominantly on encephalographic or ventriculographic findings enables formulation of a guarded prognosis."}
{"id": "PMID:1078896", "title": "Aneurysms of the small pancreatic arteries: a cause of upper abdominal pain and intestinal bleeding.", "content": "Aneurysms of the smaller splanchnic arteries, though rare, are of great significance because of the high mortality rate associated with rupture of these vessels. Such aneurysms often occur in patients with pancreatitis or pancreatic pseudocysts. Four cases are presented to demonstrate the preoperative diagnosis of this entity by selective arteriography.", "contents": "Aneurysms of the small pancreatic arteries: a cause of upper abdominal pain and intestinal bleeding. Aneurysms of the smaller splanchnic arteries, though rare, are of great significance because of the high mortality rate associated with rupture of these vessels. Such aneurysms often occur in patients with pancreatitis or pancreatic pseudocysts. Four cases are presented to demonstrate the preoperative diagnosis of this entity by selective arteriography."}
{"id": "PMID:1078897", "title": "Xerographic applications in neuroradiology. A comparison with conventional film imaging.", "content": "The capacity of electrostatic imaging for edge enhancement and wide latitude recording can improve visualization in neuroradiologic studies using low differential absorption contrast agents. Gas myelography and pneumoencephalography show the most satisfactory improvement in visualization. Tomography combined with xerography enhances definition further, but with gas myelography, especially in the thoracic region, plain xeroradiographic definition is frequently diagnostic.", "contents": "Xerographic applications in neuroradiology. A comparison with conventional film imaging. The capacity of electrostatic imaging for edge enhancement and wide latitude recording can improve visualization in neuroradiologic studies using low differential absorption contrast agents. Gas myelography and pneumoencephalography show the most satisfactory improvement in visualization. Tomography combined with xerography enhances definition further, but with gas myelography, especially in the thoracic region, plain xeroradiographic definition is frequently diagnostic."}
{"id": "PMID:1078898", "title": "Fracture of the first rib as a complication of midline sternotomy.", "content": "Fracture of the first rib occurred in six patients after cardiac surgery through a midline sternotomy. Two of the fractures could not be seen on the chest films and were demonstrated only on oblique radiographs of the cervical spine. Symptoms simulating myocardial infarction and/or pulmonary embolism were present in three of the six cases. In a review of 50 randomly selected cases of midline sternotomy, first-rib fractures were found in 3(6%).", "contents": "Fracture of the first rib as a complication of midline sternotomy. Fracture of the first rib occurred in six patients after cardiac surgery through a midline sternotomy. Two of the fractures could not be seen on the chest films and were demonstrated only on oblique radiographs of the cervical spine. Symptoms simulating myocardial infarction and/or pulmonary embolism were present in three of the six cases. In a review of 50 randomly selected cases of midline sternotomy, first-rib fractures were found in 3(6%)."}
{"id": "PMID:1078913", "title": "One hundred consecutive operations for diverticulitis of the colon.", "content": "This report describes 100 consecutive patients treated surgically for diverticulitis of the colon. The main indications for operation were recurrent attacks (33), rapid progressive symptoms (17), bleeding (16), palpable mass (14), or combinations of the above. Eighty-four patients had primary resection (two deaths), ten had staged procedure (two deaths), and six had Hartmann procedures (one death). The mortality was highest in staged procedures as this group of patients included those with complications resulting in the greatest operative risks. No deaths occurred in the elective cases. In 25 cases, various complications developed. The most common was wound infection and sepsis. The five deaths in the series are reported in detail with an evaluation of possible alternative methods of treatment. Follow-up of the series showed only one recurrence in the two years after operation.", "contents": "One hundred consecutive operations for diverticulitis of the colon. This report describes 100 consecutive patients treated surgically for diverticulitis of the colon. The main indications for operation were recurrent attacks (33), rapid progressive symptoms (17), bleeding (16), palpable mass (14), or combinations of the above. Eighty-four patients had primary resection (two deaths), ten had staged procedure (two deaths), and six had Hartmann procedures (one death). The mortality was highest in staged procedures as this group of patients included those with complications resulting in the greatest operative risks. No deaths occurred in the elective cases. In 25 cases, various complications developed. The most common was wound infection and sepsis. The five deaths in the series are reported in detail with an evaluation of possible alternative methods of treatment. Follow-up of the series showed only one recurrence in the two years after operation."}
{"id": "PMID:1078914", "title": "The clinical presentation of mesenteric vascular disease.", "content": "The clinical presentation of 17 patients with mesenteric vascular disease admitted to Mount Sinai Medical Center was reviewed. The signs and symptoms were similar in most cases. However, the acute onset of the symptom triad of abdominal pain, diarrhea, and bloody stools in an elderly patient should make one suspect the possibility of mesenteric vascular disease. The gross and light microscopic appearance of the intestinal tract was characterized by hemorrhagic infarcts regardless of the cause of the bowel ischemia. Mortality from this disease remains high, with only four of our 17 patients alive four months after operation.", "contents": "The clinical presentation of mesenteric vascular disease. The clinical presentation of 17 patients with mesenteric vascular disease admitted to Mount Sinai Medical Center was reviewed. The signs and symptoms were similar in most cases. However, the acute onset of the symptom triad of abdominal pain, diarrhea, and bloody stools in an elderly patient should make one suspect the possibility of mesenteric vascular disease. The gross and light microscopic appearance of the intestinal tract was characterized by hemorrhagic infarcts regardless of the cause of the bowel ischemia. Mortality from this disease remains high, with only four of our 17 patients alive four months after operation."}
{"id": "PMID:1078917", "title": "Slow fibers in the frog cruralis muscle.", "content": "Extrafusal slow fibers have been identified by electron microscopy in a muscle fiber bundle isolated from the anterior head (m. cruralis) of the triceps femoris of Rana pipiens. Light microscopic examination of this muscle revealed two equally numerous fiber types, one Fibrillenstruktur-like (twitch) and the other structureless (slow). A comparison of force developed by tetanic stimulation and by K+ depolarization also suggested approximately equal numbers of twitch and slow fibers.", "contents": "Slow fibers in the frog cruralis muscle. Extrafusal slow fibers have been identified by electron microscopy in a muscle fiber bundle isolated from the anterior head (m. cruralis) of the triceps femoris of Rana pipiens. Light microscopic examination of this muscle revealed two equally numerous fiber types, one Fibrillenstruktur-like (twitch) and the other structureless (slow). A comparison of force developed by tetanic stimulation and by K+ depolarization also suggested approximately equal numbers of twitch and slow fibers."}
{"id": "PMID:1078919", "title": "[Variability of heat resistance of frog muscle tissue in Ringer's solution].", "content": "The variability in the initial level of heat resistance of muscles was found to decrease with increase in the duration of keeping the frogs in vivarium. However, the variability in the resistance of isolated muscles kept in Ringer's solution during 90 min. is independent of this factor and lesser than the initial one. The data obtained suggest that the narrowing of the variability in the resistance of muscles kept in Ringer's solution is determined by the dispersion of the initial level of the resistance. This phenomenon is only observed when the individual variability in the resistance of muscles is sufficiently high immediately after their isolation.", "contents": "[Variability of heat resistance of frog muscle tissue in Ringer's solution]. The variability in the initial level of heat resistance of muscles was found to decrease with increase in the duration of keeping the frogs in vivarium. However, the variability in the resistance of isolated muscles kept in Ringer's solution during 90 min. is independent of this factor and lesser than the initial one. The data obtained suggest that the narrowing of the variability in the resistance of muscles kept in Ringer's solution is determined by the dispersion of the initial level of the resistance. This phenomenon is only observed when the individual variability in the resistance of muscles is sufficiently high immediately after their isolation."}
{"id": "PMID:1078920", "title": "Stages of vegatative afferentation in reorganization of bladder control during intravesical electrotherapy.", "content": "420 patients were treated with transurethral, intravesical electrotherapy to improve the function of paralyzed urinary bladder. Normal conscious micturition control was obtained by 314 patients. This electrotherapy contrasts sharply in method and goal with all implanted pacemakers or inserted electric plugs. Nor does the therapy have anything in common with various bladder training methods. The aim of therapy is to recreate vegetative afferentation from the bladder to the CNS. Reactivation of the intramural bladder receptors and facilitation processes plays an important part in establishing normal bladder control.", "contents": "Stages of vegatative afferentation in reorganization of bladder control during intravesical electrotherapy. 420 patients were treated with transurethral, intravesical electrotherapy to improve the function of paralyzed urinary bladder. Normal conscious micturition control was obtained by 314 patients. This electrotherapy contrasts sharply in method and goal with all implanted pacemakers or inserted electric plugs. Nor does the therapy have anything in common with various bladder training methods. The aim of therapy is to recreate vegetative afferentation from the bladder to the CNS. Reactivation of the intramural bladder receptors and facilitation processes plays an important part in establishing normal bladder control."}
{"id": "PMID:1078924", "title": "[The presence of scleroderma and chronic lymphadenosis concurrently in a patient (author's transl)].", "content": "This report deals with the case of a 50 year-old woman with long-standing scleroderma of the acral-asphyxial type with a relatively slight tendency to progression. Chronic lymphadenosis has been an additional feature of this case over the past few years. The following findings appear of particular interest: autoimmune phenomena in the form of antinuclear antibodies, a virtually monoclonal proliferation of B-lymphocytes and a relative T-lymphocyte deficiency. It is suggested that both conditions may be pathogenetically connected with an immunologically defined insufficiency of the lymphocytic system. Hence, in view of this observation, it may not be correct to consider scleroderma a \"paraneoplastic phenomenon\".", "contents": "[The presence of scleroderma and chronic lymphadenosis concurrently in a patient (author's transl)]. This report deals with the case of a 50 year-old woman with long-standing scleroderma of the acral-asphyxial type with a relatively slight tendency to progression. Chronic lymphadenosis has been an additional feature of this case over the past few years. The following findings appear of particular interest: autoimmune phenomena in the form of antinuclear antibodies, a virtually monoclonal proliferation of B-lymphocytes and a relative T-lymphocyte deficiency. It is suggested that both conditions may be pathogenetically connected with an immunologically defined insufficiency of the lymphocytic system. Hence, in view of this observation, it may not be correct to consider scleroderma a \"paraneoplastic phenomenon\"."}
{"id": "PMID:1078929", "title": "Coronary artery anatomy before and after direct revascularization surgery: clinical and cinearteriographic studies in 67 selected patients.", "content": "This report relates the postoperative clinical and cineangiographic status of 67 patients selected from a total of 202 patients who underwent coronary artery surgery at the Peter Bent Brigham Hospital from July, 1970, to July, 1972. The mean interval after operation was 12.6 months. Ninety-one per cent of the 67 patients were improved from their preoperative status. Forty-eight patients (71 per cent) were studied to evaluate recurrence of mild to moderate angina or occurrence of interval myocardial infarction, and 19 patients (29 per cent) were entirely asymptomatic. In the 67 patients studied, 112 coronary arteries received a total of 115 grafts. (There were 89 ungrafted coronary arteries.) Total graft patency rate for the 58 patients in whom angina was totally or significantly relieved was 65 per cent. However, one or more grafts were patent in 52 (90 per cent) of these 58 patients. In grafted arteries, progression was found in segments proximal to the graft in 37 per cent of arteries, at the site of anastomosis in 10 per cent, and distal to the site of anastomosis in 17 per cent. The frequency of obstruction distal to the site of anastomosis was not significantly different from the frequency of progression in nongrafted arteries, in contrast to preliminary data from this laboratory. Overall and regional progression in grafted arteries appeared to occur primarily within the first four months after surgery and was found, thereafter, in a constand percentage of vessels studied. Progression in coronary arteries was independent of patency or occlusion of the graft to the vessel. It is hypothesized that while proximal progression is probably a consequence of altered hydraulic factors, distal lesions seem to represent natural progression of atherosclerotic disease.", "contents": "Coronary artery anatomy before and after direct revascularization surgery: clinical and cinearteriographic studies in 67 selected patients. This report relates the postoperative clinical and cineangiographic status of 67 patients selected from a total of 202 patients who underwent coronary artery surgery at the Peter Bent Brigham Hospital from July, 1970, to July, 1972. The mean interval after operation was 12.6 months. Ninety-one per cent of the 67 patients were improved from their preoperative status. Forty-eight patients (71 per cent) were studied to evaluate recurrence of mild to moderate angina or occurrence of interval myocardial infarction, and 19 patients (29 per cent) were entirely asymptomatic. In the 67 patients studied, 112 coronary arteries received a total of 115 grafts. (There were 89 ungrafted coronary arteries.) Total graft patency rate for the 58 patients in whom angina was totally or significantly relieved was 65 per cent. However, one or more grafts were patent in 52 (90 per cent) of these 58 patients. In grafted arteries, progression was found in segments proximal to the graft in 37 per cent of arteries, at the site of anastomosis in 10 per cent, and distal to the site of anastomosis in 17 per cent. The frequency of obstruction distal to the site of anastomosis was not significantly different from the frequency of progression in nongrafted arteries, in contrast to preliminary data from this laboratory. Overall and regional progression in grafted arteries appeared to occur primarily within the first four months after surgery and was found, thereafter, in a constand percentage of vessels studied. Progression in coronary arteries was independent of patency or occlusion of the graft to the vessel. It is hypothesized that while proximal progression is probably a consequence of altered hydraulic factors, distal lesions seem to represent natural progression of atherosclerotic disease."}
{"id": "PMID:1078930", "title": "Variant angina pectoris: a clinical and coronary arteriographic spectrum.", "content": "The clinical course and coronary arteriographic findings in eight patients with Prinzmetal's variant angina pectoris are reviewed and contrasted to previously reported cases.  In six patients with S-T-elevation inferiorly, three had normal coronary arteriograms, one had complete right coronary artery occlusion, one had diffuse triple-vessel disease, and one did not undergo coronary arteriography.  In two patients with S-T-elevation anteriorly, severe stenosis of the anterior descending coronary artery was present.  Medical treatment in four patients and surgical revascularization of the anterior descending coronary artery in two patients were both accompanied by marked symptomatic improvement.  Spontaneous loss of angina occurred in two patients.  During 17 months' mean follow-up, seven patients have remained free of angina and one died suddenly.  Variant angina pectoris may be accompanied by a variety of coronary arteriographic findings and the prognosis appears more favorable than previously reported.", "contents": "Variant angina pectoris: a clinical and coronary arteriographic spectrum. The clinical course and coronary arteriographic findings in eight patients with Prinzmetal's variant angina pectoris are reviewed and contrasted to previously reported cases.  In six patients with S-T-elevation inferiorly, three had normal coronary arteriograms, one had complete right coronary artery occlusion, one had diffuse triple-vessel disease, and one did not undergo coronary arteriography.  In two patients with S-T-elevation anteriorly, severe stenosis of the anterior descending coronary artery was present.  Medical treatment in four patients and surgical revascularization of the anterior descending coronary artery in two patients were both accompanied by marked symptomatic improvement.  Spontaneous loss of angina occurred in two patients.  During 17 months' mean follow-up, seven patients have remained free of angina and one died suddenly.  Variant angina pectoris may be accompanied by a variety of coronary arteriographic findings and the prognosis appears more favorable than previously reported."}
{"id": "PMID:1078932", "title": "Bilateral arteriosclerotic coronary arterial aneurysms successfully treated with saphenous vein bypass grafting.", "content": "A man with ischemic heart disease was discovered to have bilateral coronary arterial aneurysms that were sucessfully treated with saphenous vein grafting. Preoperative coronary arteriograms revealed a tight luminal stenosis at the proximal end of the large aneurysm in the left anterior descending artery, suggesting poststenotic dilatation as an etiologic or contributing factor. The right coronary artery was totally occluded proximally, but was noted to be aneurysmal at operation. This is the 14th published case of nonfistulous coronary arterial aneurysm diagnosed in vivo, and the first associated with proximal luminal stenosis.", "contents": "Bilateral arteriosclerotic coronary arterial aneurysms successfully treated with saphenous vein bypass grafting. A man with ischemic heart disease was discovered to have bilateral coronary arterial aneurysms that were sucessfully treated with saphenous vein grafting. Preoperative coronary arteriograms revealed a tight luminal stenosis at the proximal end of the large aneurysm in the left anterior descending artery, suggesting poststenotic dilatation as an etiologic or contributing factor. The right coronary artery was totally occluded proximally, but was noted to be aneurysmal at operation. This is the 14th published case of nonfistulous coronary arterial aneurysm diagnosed in vivo, and the first associated with proximal luminal stenosis."}
{"id": "PMID:1078933", "title": "Bilateral nonfistulous congenital coronary arterial aneurysms.", "content": "A 15 year old boy collapsed and died after participating in a basketball game. Autopsy revealed bilateral congenital coronary arterial aneurysms. The diagnosis was made post mortem but, retrospectively, might have been suspected during life, even before angiography. The clues to the correct diagnosis were chest pain, a systolic and diastolic murmur and a mass on the right heart border in the chest roentgenogram.", "contents": "Bilateral nonfistulous congenital coronary arterial aneurysms. A 15 year old boy collapsed and died after participating in a basketball game. Autopsy revealed bilateral congenital coronary arterial aneurysms. The diagnosis was made post mortem but, retrospectively, might have been suspected during life, even before angiography. The clues to the correct diagnosis were chest pain, a systolic and diastolic murmur and a mass on the right heart border in the chest roentgenogram."}
{"id": "PMID:1078934", "title": "Ampicillin in Haemophilus influenzae meningitis. Clinicopharmacologic evaluation of intramuscular vs intravenous administration.", "content": "Sixty-two children with Haemophilus meningitis were treated with ampicillin sodium, 200 mg/kg/day for ten days. Thirty-one received the drug intravenously (IV) for ten days; the other 31 patients received ampicillin IV for five days (except for one test dose given intramuscularly [IM] on day 2) followed by IM administration of ampicillin the last five days. Ampicillin concentrations in cerebrospinal fluid (SCF) were higher one hour after IV administration, but at two and four hours, concentrations were greater after IM doses. Responses to therapy and rates of complications were similar in the two groups. Fourteen patients had positive CSF cultures on day 2. Ten of these were isolated only on Levinthal medium and would have been overlooked with routine culture methods. All organisms were ampicillin-susceptible and all CSF cultures were negative by 48 hours. More patients with delayed sterilization had neurologic sequelae (78%) than patients with prompt CSF sterilization (13%). The schedule of five days of IV treatment followed by five days of IM therapy was pharmacologically and clinically as effective as ten days of IV therapy and has practical advantages.", "contents": "Ampicillin in Haemophilus influenzae meningitis. Clinicopharmacologic evaluation of intramuscular vs intravenous administration. Sixty-two children with Haemophilus meningitis were treated with ampicillin sodium, 200 mg/kg/day for ten days. Thirty-one received the drug intravenously (IV) for ten days; the other 31 patients received ampicillin IV for five days (except for one test dose given intramuscularly [IM] on day 2) followed by IM administration of ampicillin the last five days. Ampicillin concentrations in cerebrospinal fluid (SCF) were higher one hour after IV administration, but at two and four hours, concentrations were greater after IM doses. Responses to therapy and rates of complications were similar in the two groups. Fourteen patients had positive CSF cultures on day 2. Ten of these were isolated only on Levinthal medium and would have been overlooked with routine culture methods. All organisms were ampicillin-susceptible and all CSF cultures were negative by 48 hours. More patients with delayed sterilization had neurologic sequelae (78%) than patients with prompt CSF sterilization (13%). The schedule of five days of IV treatment followed by five days of IM therapy was pharmacologically and clinically as effective as ten days of IV therapy and has practical advantages."}
{"id": "PMID:1078935", "title": "Crohn's disease of the colon. II. Controversial aspects of hemorrhage, anemia and rectal involvement in granulomatous disease involving the colon.", "content": "In 160 cases with granulomatous colitis or ileocolitis, blood was found in the stool in 72 or 45%. Massive bleeding occurred in four patients (25%); overt bleeding in 51 (32%) and occult bleeding, repeated guaiac positive stools, in 17 (11%). Thus, clinically obvious bleeding occurred in about one patient in three. Of the four patients with massive hemorrhage one required subtotal colectomy, one right hemicolectomy and the other two were controlled by medical measures including blood transfusion. In each of the surgical cases, the massive bleeding originated in the region of the cecum. An additional patient, not in the series, required proctectomy as a life-saving measure following subtotal colectomy. Clinically obvious rectal bleeding in Crohn's colitis is approximately ten times as frequent as in regional enteritis but only one-third as frequent as in ulcerative colitis. There was no correlation between rectal bleeding and rectal involvement determined radiologically or by signoidoscopy, in the whole series but there was a strong association in the small group of Crohn's colitis with rectal involvement (86% with bleeding) and a significantly greater proportion of patients with colitis with rectal involvement and hemorrhage (P smaller than 0.02). Anemia is common in both groups but in Crohn's colitis it is almost always, 75%, associated with rectal blood loss, whereas in ileocolitis this association is much less marked, 40%, indicating other important causes of anemia when there is small bowel disease. The bleeding patient with Crohn's colitis should be managed medically initially. Uncontrollable, continuous, or massive hemorrhage may require angiography and early surgical intervention.", "contents": "Crohn's disease of the colon. II. Controversial aspects of hemorrhage, anemia and rectal involvement in granulomatous disease involving the colon. In 160 cases with granulomatous colitis or ileocolitis, blood was found in the stool in 72 or 45%. Massive bleeding occurred in four patients (25%); overt bleeding in 51 (32%) and occult bleeding, repeated guaiac positive stools, in 17 (11%). Thus, clinically obvious bleeding occurred in about one patient in three. Of the four patients with massive hemorrhage one required subtotal colectomy, one right hemicolectomy and the other two were controlled by medical measures including blood transfusion. In each of the surgical cases, the massive bleeding originated in the region of the cecum. An additional patient, not in the series, required proctectomy as a life-saving measure following subtotal colectomy. Clinically obvious rectal bleeding in Crohn's colitis is approximately ten times as frequent as in regional enteritis but only one-third as frequent as in ulcerative colitis. There was no correlation between rectal bleeding and rectal involvement determined radiologically or by signoidoscopy, in the whole series but there was a strong association in the small group of Crohn's colitis with rectal involvement (86% with bleeding) and a significantly greater proportion of patients with colitis with rectal involvement and hemorrhage (P smaller than 0.02). Anemia is common in both groups but in Crohn's colitis it is almost always, 75%, associated with rectal blood loss, whereas in ileocolitis this association is much less marked, 40%, indicating other important causes of anemia when there is small bowel disease. The bleeding patient with Crohn's colitis should be managed medically initially. Uncontrollable, continuous, or massive hemorrhage may require angiography and early surgical intervention."}
{"id": "PMID:1078936", "title": "Hemorrhagic erosive gastritis.  A clinical study.", "content": "Four hundred consecutive patients, in a representative Southern California population, hospitalized for upper gastrointestinal hemorrhage, had endoscopic examinations performed within 24 hours of arrival in the emergency room.  Seventy-four (18.5 percent) of these were found to have the typical lesions of acute hemorrhagic erosive gastritis.  The clinical spectrum of this group is examined and a comparison made with a Veterans Hospital population reported previously.", "contents": "Hemorrhagic erosive gastritis.  A clinical study. Four hundred consecutive patients, in a representative Southern California population, hospitalized for upper gastrointestinal hemorrhage, had endoscopic examinations performed within 24 hours of arrival in the emergency room.  Seventy-four (18.5 percent) of these were found to have the typical lesions of acute hemorrhagic erosive gastritis.  The clinical spectrum of this group is examined and a comparison made with a Veterans Hospital population reported previously."}
{"id": "PMID:1078937", "title": "Gastric mucosal lacerations from blunt trauma to the abdomen.", "content": "A 17-year old, Caucasian male, high school student sustained gastric mucosal lacerations from blunt trauma to the abdomen during a football game with associated severe upper gastrointestinal bleeding.  The lacerations were diagnosed by endoscopy and treated conservatively with uneventful, rapid and complete recovery.", "contents": "Gastric mucosal lacerations from blunt trauma to the abdomen. A 17-year old, Caucasian male, high school student sustained gastric mucosal lacerations from blunt trauma to the abdomen during a football game with associated severe upper gastrointestinal bleeding.  The lacerations were diagnosed by endoscopy and treated conservatively with uneventful, rapid and complete recovery."}
{"id": "PMID:1078938", "title": "Atypical retinitis pigmentosa masquerading as a nerve fiber bundle lesion.", "content": "\"Sector\" or \"asymmetric\" retinitis pigmentosa was mistaken as bilateral nerve fiber bundle defects in a 56-year-old man for six years, leading to an unnecessary work-up for anterior visual pathway disease and to an incorrect diagnosis of low-tension glaucoma. Confusion arises because this variant may present with  bilateral, superior, half-ring visual field defects that intersect the blindspots. These defects may be misplotted as typical arcuate Bjerrum's scotomas. The ophthalmoscopic changes that correspond to these visual field defects may be so subtle as to be overlooked. Fluorescein angiography helps bring out the retinal lesions, and moderately subnormal electroretinographic and dark adaptation studies are definitive.", "contents": "Atypical retinitis pigmentosa masquerading as a nerve fiber bundle lesion. \"Sector\" or \"asymmetric\" retinitis pigmentosa was mistaken as bilateral nerve fiber bundle defects in a 56-year-old man for six years, leading to an unnecessary work-up for anterior visual pathway disease and to an incorrect diagnosis of low-tension glaucoma. Confusion arises because this variant may present with  bilateral, superior, half-ring visual field defects that intersect the blindspots. These defects may be misplotted as typical arcuate Bjerrum's scotomas. The ophthalmoscopic changes that correspond to these visual field defects may be so subtle as to be overlooked. Fluorescein angiography helps bring out the retinal lesions, and moderately subnormal electroretinographic and dark adaptation studies are definitive."}
{"id": "PMID:1078939", "title": "Exogenous fungal endophthalmitis caused by Paecilomyces.", "content": "A 17-year-old white boy developed a fulminating corneal infection and endophthalmitis in his left eye after trauma. He was treated with antibiotics and corticosteroids for one week prior to diagnosis. A saprophytic fungus, Paecilomyces viridis, was cultured from corneal scrapings and was demonstrated in the vitreous cavity by histopathologic examination.", "contents": "Exogenous fungal endophthalmitis caused by Paecilomyces. A 17-year-old white boy developed a fulminating corneal infection and endophthalmitis in his left eye after trauma. He was treated with antibiotics and corticosteroids for one week prior to diagnosis. A saprophytic fungus, Paecilomyces viridis, was cultured from corneal scrapings and was demonstrated in the vitreous cavity by histopathologic examination."}
{"id": "PMID:1078942", "title": "Ba++ on the resting frog stomach: effects on electrical and secretory parameters.", "content": "Ba++ added to the nutrient solution bathing the resting frog stomach increased resistance, decreased the PD, and stimulated acid secretion. Under short-circuit conditions, the increase in H+-secretory rate was accompanied by a decrease in short-circuit current (I-sc). These changes were reversed by NaSCN (10 mM), suggesting that Ba++ had not impaired the current-generating mechanism per se. Histamine-induced acid secretion was associated with an increase in net Cl- flux, particularly in the N yields S flux (JNS). Ba++ increased acid secretion with no increase in JNS and a decrease in net Cl- flux. The effects of Ba++ were amplified by low-Cl- solutions. Histamine, in the presence of Ba++ and low-Cl- solutions, increased acid secretion and transmucosal resistance, suggesting the operation of a neutral pump in the secretion of HCl. It is concluded that Ba++ limits Cl- entry and also acts as a secretagogue.", "contents": "Ba++ on the resting frog stomach: effects on electrical and secretory parameters. Ba++ added to the nutrient solution bathing the resting frog stomach increased resistance, decreased the PD, and stimulated acid secretion. Under short-circuit conditions, the increase in H+-secretory rate was accompanied by a decrease in short-circuit current (I-sc). These changes were reversed by NaSCN (10 mM), suggesting that Ba++ had not impaired the current-generating mechanism per se. Histamine-induced acid secretion was associated with an increase in net Cl- flux, particularly in the N yields S flux (JNS). Ba++ increased acid secretion with no increase in JNS and a decrease in net Cl- flux. The effects of Ba++ were amplified by low-Cl- solutions. Histamine, in the presence of Ba++ and low-Cl- solutions, increased acid secretion and transmucosal resistance, suggesting the operation of a neutral pump in the secretion of HCl. It is concluded that Ba++ limits Cl- entry and also acts as a secretagogue."}
{"id": "PMID:1078943", "title": "Effects of bile salts on amphibian gastric mucosa.", "content": "The effect of five different bile salts on the mucosal or serosal surface of isolated bullfrog gastric mucosa was investigated. Serosal exposure (30 min) to 15-40 mM taurocholate (TCA) or taurodeoxycholate (TDCA), 10-40 mM deoxycholate (DCA), or 2.5-7.5 mM cholate (CA) typically produced a marked increase in resistance (R) and a decrease in potential difference (PD), short-circuit current (I-sc), and H+ secretion. Flux of 36Cl from serosal to mucosal solution decreased progressively. The flux of [14C]sucrose across the mucosa was slightly decreased. Microscopy showed condensation of the parietal cells. Mucosal exposure (30 min) at concentrations above 2.5 mM DCA or CA and 15 mM TCA and TDCA caused a marked decrease in R, PD, I-sc, and H+ secretion, and the flux of [14C]sucrose across the mucosa was increased. Extreme destruction of the surface mucous cells was the primary histologic finding. The effect of dehydrocholate was identical to that of TCA. Mucosal bile salts cause an increased permeability primarily by injury of the surface epithelium whereas serosal bile salts affected primarily the oxyntic cells.", "contents": "Effects of bile salts on amphibian gastric mucosa. The effect of five different bile salts on the mucosal or serosal surface of isolated bullfrog gastric mucosa was investigated. Serosal exposure (30 min) to 15-40 mM taurocholate (TCA) or taurodeoxycholate (TDCA), 10-40 mM deoxycholate (DCA), or 2.5-7.5 mM cholate (CA) typically produced a marked increase in resistance (R) and a decrease in potential difference (PD), short-circuit current (I-sc), and H+ secretion. Flux of 36Cl from serosal to mucosal solution decreased progressively. The flux of [14C]sucrose across the mucosa was slightly decreased. Microscopy showed condensation of the parietal cells. Mucosal exposure (30 min) at concentrations above 2.5 mM DCA or CA and 15 mM TCA and TDCA caused a marked decrease in R, PD, I-sc, and H+ secretion, and the flux of [14C]sucrose across the mucosa was increased. Extreme destruction of the surface mucous cells was the primary histologic finding. The effect of dehydrocholate was identical to that of TCA. Mucosal bile salts cause an increased permeability primarily by injury of the surface epithelium whereas serosal bile salts affected primarily the oxyntic cells."}
{"id": "PMID:1078944", "title": "Angiography of cerebellar hemorrhage secondary to hypertension.", "content": "In a patient with cerebellar hemorrhage due to hypertension, deterioration may be so rapid there is not sufficient time for radiographic studies prior to craniectomy. In the less critically ill patient, posterior fossa angiography is the procedure of choice and is highly accurate in localizing the mass lesion. Angiography is particularly helpful in the patient with an atypical presentation because an etiology of the hemorrhage other than hypertension may be demonstrated.", "contents": "Angiography of cerebellar hemorrhage secondary to hypertension. In a patient with cerebellar hemorrhage due to hypertension, deterioration may be so rapid there is not sufficient time for radiographic studies prior to craniectomy. In the less critically ill patient, posterior fossa angiography is the procedure of choice and is highly accurate in localizing the mass lesion. Angiography is particularly helpful in the patient with an atypical presentation because an etiology of the hemorrhage other than hypertension may be demonstrated."}
{"id": "PMID:1078945", "title": "Isoproterenol in offsetting adverse effects of vasopressin in cirrhotic patients.", "content": "Vasopressin administered as a peripheral infusion (40 U/hr) significantly reduced portal vein pressure in ten awake patients with cirrhosis and portal hypertension. A vasopressin-induced reduction in cardiac output occurred in five of the ten patients (50 per cent). Vasopressin-induced changes in systemic arterial pressure, heart rate, and portal venous pressure were independent of alterations in cardiac output. When the five patients with vasopressin-induced reductions in cardiac output were given a combination of vasopressin and isoproterenol, cardiac output was maintained and the reduction in portal vein pressure was equal to that observed with unopposed vasopressin therapy. Thus, the addition of isoproterenol prevented a vasopressin-induced reduction in cardiac output while permitting vasopressin to reduce portal vein pressure.", "contents": "Isoproterenol in offsetting adverse effects of vasopressin in cirrhotic patients. Vasopressin administered as a peripheral infusion (40 U/hr) significantly reduced portal vein pressure in ten awake patients with cirrhosis and portal hypertension. A vasopressin-induced reduction in cardiac output occurred in five of the ten patients (50 per cent). Vasopressin-induced changes in systemic arterial pressure, heart rate, and portal venous pressure were independent of alterations in cardiac output. When the five patients with vasopressin-induced reductions in cardiac output were given a combination of vasopressin and isoproterenol, cardiac output was maintained and the reduction in portal vein pressure was equal to that observed with unopposed vasopressin therapy. Thus, the addition of isoproterenol prevented a vasopressin-induced reduction in cardiac output while permitting vasopressin to reduce portal vein pressure."}
{"id": "PMID:1078946", "title": "Mesenteric arterial infusions of vasopressin for hemorrhage from colonic diverticulosis.", "content": "Twenty-four patients with massive rectal hemorrhage and known or subsequently proved colonic diverticular disease had the bleeding site localized by mesenteric angiography and received intra-arterial infusion of vasopressin to arrest the bleeding. In twenty-two patients the bleeding was controlled with the vasopressin infusion whereas in the remaining two, hemorrhage did not stop and surgery was performed. Of the twenty-two patients in whom bleeding was arrested by vasopressin infusion, twelve received no further surgical therapy, five had elective prophylactic surgical resection after a period of hemostasis, and the remaining five underwent segmental resection for bleeding that recurred after cessation of the infusion. Of the twelve patients who were not operated on, three had rebleeding two, four, and twelve months after vasopressin infusion and two of these three patients required surgery. The remaining nine have had no recurrent bleeding for periods ranging from seven to thirty-four months. Of ten patients who had segmental resection after precise localization of the bleeding site and initial control with vasopressin, no one has had recurrent hemorrhage for periods ranging from two to eighteen months.", "contents": "Mesenteric arterial infusions of vasopressin for hemorrhage from colonic diverticulosis. Twenty-four patients with massive rectal hemorrhage and known or subsequently proved colonic diverticular disease had the bleeding site localized by mesenteric angiography and received intra-arterial infusion of vasopressin to arrest the bleeding. In twenty-two patients the bleeding was controlled with the vasopressin infusion whereas in the remaining two, hemorrhage did not stop and surgery was performed. Of the twenty-two patients in whom bleeding was arrested by vasopressin infusion, twelve received no further surgical therapy, five had elective prophylactic surgical resection after a period of hemostasis, and the remaining five underwent segmental resection for bleeding that recurred after cessation of the infusion. Of the twelve patients who were not operated on, three had rebleeding two, four, and twelve months after vasopressin infusion and two of these three patients required surgery. The remaining nine have had no recurrent bleeding for periods ranging from seven to thirty-four months. Of ten patients who had segmental resection after precise localization of the bleeding site and initial control with vasopressin, no one has had recurrent hemorrhage for periods ranging from two to eighteen months."}
{"id": "PMID:1078947", "title": "Duodenal hematoma. Critical analysis of recent treatment technics.", "content": "Analysis of the fifty-six cases of duodenal hematoma reported since 1966 shows a significant rise in incidence, with uncertainty regarding the most appropriate approaches to therapy. The diagnosis can generally be made by recognizing the \"coil spring\" sign on upper gastrointestinal series. Thirty-four per cent of the patients reviewed were treated successfully with conservative management. Sixty-two per cent were treated surgically. Fifty-one per cent of the surgical patients were treated by simple evacuation of the hematoma, and 12 per cent of these required reoperation for recurrent obstruction. Forty per cent of the surgical patients were treated by evacuation and gastrojejunostomy, and 21 per cent of these required reoperation for marginal ulceration. A previously unreported case is presented and a new surgical approach utilizing gastroduodenostomy is described, which is believed to offer potentially less risk of subsequent marginal ulceration or recurrent duodenal obstruction.", "contents": "Duodenal hematoma. Critical analysis of recent treatment technics. Analysis of the fifty-six cases of duodenal hematoma reported since 1966 shows a significant rise in incidence, with uncertainty regarding the most appropriate approaches to therapy. The diagnosis can generally be made by recognizing the \"coil spring\" sign on upper gastrointestinal series. Thirty-four per cent of the patients reviewed were treated successfully with conservative management. Sixty-two per cent were treated surgically. Fifty-one per cent of the surgical patients were treated by simple evacuation of the hematoma, and 12 per cent of these required reoperation for recurrent obstruction. Forty per cent of the surgical patients were treated by evacuation and gastrojejunostomy, and 21 per cent of these required reoperation for marginal ulceration. A previously unreported case is presented and a new surgical approach utilizing gastroduodenostomy is described, which is believed to offer potentially less risk of subsequent marginal ulceration or recurrent duodenal obstruction."}
{"id": "PMID:1078948", "title": "Clinical appearance of optic disc drusen in childhood.", "content": "In a series of 50 individuals with optic disc drusen diagnosed before the age of 15, the anomaly occurred most often in the form of an elevation of the whole or one part of the optic disc without visible superficial drusen. Superficial drusen were seen only in 15 of the 92 eyes with the anomaly. In those 42 subjects in whom the anomaly was bilateral, it was seldom symmetrical. The reduced visual acuity found in a few cases could be attributed to causes other than the drusen. In 10 eyes the anomaly was observed to be associated with visual field defects. The age distributions of the subjects with visual field defects and with superficial drusen show that they were more common among older children. The superficial position of the drusen and the presence of visual field defects do not, however, have a close cause-and-effect relationship. A cilioretinal artery was observed to co-exist with optic disc drusen with a high frequency, i.e. in more than 40 per cent. Other vascular aberrations were also noted in association with the optic disc anomaly. Neurological symptoms were the most common cause for consultation in the 50 children examined. In more than half of the present cases appearance of the optic disc had contributed to the suspicion of an intracranial expansive process. A fallacious similarity between the elevation of the optic disc caused by buried drusen and optic disc edema caused by increased intracranial pressure presents a problem of differential diagnosis, expressly in younger children.", "contents": "Clinical appearance of optic disc drusen in childhood. In a series of 50 individuals with optic disc drusen diagnosed before the age of 15, the anomaly occurred most often in the form of an elevation of the whole or one part of the optic disc without visible superficial drusen. Superficial drusen were seen only in 15 of the 92 eyes with the anomaly. In those 42 subjects in whom the anomaly was bilateral, it was seldom symmetrical. The reduced visual acuity found in a few cases could be attributed to causes other than the drusen. In 10 eyes the anomaly was observed to be associated with visual field defects. The age distributions of the subjects with visual field defects and with superficial drusen show that they were more common among older children. The superficial position of the drusen and the presence of visual field defects do not, however, have a close cause-and-effect relationship. A cilioretinal artery was observed to co-exist with optic disc drusen with a high frequency, i.e. in more than 40 per cent. Other vascular aberrations were also noted in association with the optic disc anomaly. Neurological symptoms were the most common cause for consultation in the 50 children examined. In more than half of the present cases appearance of the optic disc had contributed to the suspicion of an intracranial expansive process. A fallacious similarity between the elevation of the optic disc caused by buried drusen and optic disc edema caused by increased intracranial pressure presents a problem of differential diagnosis, expressly in younger children."}
{"id": "PMID:1078949", "title": "[Scanning electron microscopy of the zonula apparatus of higher primates. A further approach to a new theory of accommodation (author's transl)].", "content": "The architecture of the zonula apparatus of two species of monkeys is studied by scanning electron microscopy. Some specimens were treated with pilocarpine respectively atropine immediately before enucleation. The zonula consists of two functionally different groups of fiber strands, i.e. span and holding bundles (after Rohen and Rentsch), the course and structure of which is described in detail. After application of atropine the \"holding bundles\" appear to be stretched in the area between the ciliary processes and the lens, whereas after pilocarpine application they appear loose and irregularly arranged. Screw-like, spiral fiber strands were often seen in the scanning electron microscope. It is assumed that the spiral fibers develop in the stage of relaxation of the zonula apparatus.", "contents": "[Scanning electron microscopy of the zonula apparatus of higher primates. A further approach to a new theory of accommodation (author's transl)]. The architecture of the zonula apparatus of two species of monkeys is studied by scanning electron microscopy. Some specimens were treated with pilocarpine respectively atropine immediately before enucleation. The zonula consists of two functionally different groups of fiber strands, i.e. span and holding bundles (after Rohen and Rentsch), the course and structure of which is described in detail. After application of atropine the \"holding bundles\" appear to be stretched in the area between the ciliary processes and the lens, whereas after pilocarpine application they appear loose and irregularly arranged. Screw-like, spiral fiber strands were often seen in the scanning electron microscope. It is assumed that the spiral fibers develop in the stage of relaxation of the zonula apparatus."}
{"id": "PMID:1078950", "title": "[Gross anatomy of the vitreous body (author's transl)].", "content": "The anatomical arrangement of vitreous structures in human and animal eyes is described on the basis of coinciding findings of histological examinations, biomicroscopy and the \"optical dissection\" of unfixed vitreous specimens. The \"funnel\" of the primary vascular vitreous disappears completely during development. The vitreous cavity is filled with secondary vitreous. Its structure in nearly homogeneous, containing \"holes\" of low density at all sites where the structure of a normal inner retinal surface is altered. During postnatal development the secondary vitreous forms a new funnel system, consisting of zones of different density and of membranelles (vitreous tracts). Before completion of this funnel system, destruction of the vitreous gel begins and confuses the typical arrangement. Postnatal vitreous structures include: zones of different density: preretinal zone, intermediary zone, retrolental zone; holes (of low density): prepapillary hole, prefoveal hole, prevascular fissures, vitreous tracts (of high density): preretinal tract, median tract, coronary tract, retrolental tract (\"hyaloid\" tract). The pattern of the \"holes\" strongly suggests that the framework is formed by retinal elements.", "contents": "[Gross anatomy of the vitreous body (author's transl)]. The anatomical arrangement of vitreous structures in human and animal eyes is described on the basis of coinciding findings of histological examinations, biomicroscopy and the \"optical dissection\" of unfixed vitreous specimens. The \"funnel\" of the primary vascular vitreous disappears completely during development. The vitreous cavity is filled with secondary vitreous. Its structure in nearly homogeneous, containing \"holes\" of low density at all sites where the structure of a normal inner retinal surface is altered. During postnatal development the secondary vitreous forms a new funnel system, consisting of zones of different density and of membranelles (vitreous tracts). Before completion of this funnel system, destruction of the vitreous gel begins and confuses the typical arrangement. Postnatal vitreous structures include: zones of different density: preretinal zone, intermediary zone, retrolental zone; holes (of low density): prepapillary hole, prefoveal hole, prevascular fissures, vitreous tracts (of high density): preretinal tract, median tract, coronary tract, retrolental tract (\"hyaloid\" tract). The pattern of the \"holes\" strongly suggests that the framework is formed by retinal elements."}
{"id": "PMID:1078951", "title": "[Inhibition of corneal metabolism by glucocorticosteroids. An in vitro study (author's transl)]?14Hemmung des Hornhautstoffwechals durch Glukocorticosteroide. Eine in vitro Studie.", "content": "Cortisone and its derivates demonstrates influence to the metabolism of connective tissue. In this in vitro-study the action of water soluble Dexamethason (Schering) to corneal tissue is quantitatively determined. Incorporation of radioactive sulfate into the glycosaminoglycanes and the sulfate-hexosamine-quotient of keratansulfate and chondroitinsulfate is pointed out. 1. A 2 times 10-7 M Dexamethason-concentration and 6 hours incubation time causes reducing of sulfate incorporation in the case of keratansulfate (I) about 94 per cent and chondroitinsulfate (II) about 89 percent compared to nontreated control. Increasing concentration of Dexamethason leads the inhibition of sulfate incorporation to 66 per cent (I) and 63 per cent (II) respectively. Removing the epithelium from the stroma before incubation the Dexamethason concentration and 6 h incubation time only 84 percent and 80 per cent radioactive sulfate is found in keratansulfate and chondroitinsulfate. An increasing Dexamethason concentration leads to inhibition of 55 per cent (I) and 46 per cent (II) respectively. 2. A comparable inhibition of sulfate incorporation is demonstrated with constant Dexamethason concentration and variable duration of incubation. A 1 hour incubation reduced incorporation of sulfate to 89 per cent for keratansulfate and 81 per cent for chondroitinsulfate. The 24 hours incubation inhibits incorporation to 69 per cent (I) and 65 per cent (II) respectively.", "contents": "[Inhibition of corneal metabolism by glucocorticosteroids. An in vitro study (author's transl)]?14Hemmung des Hornhautstoffwechals durch Glukocorticosteroide. Eine in vitro Studie. Cortisone and its derivates demonstrates influence to the metabolism of connective tissue. In this in vitro-study the action of water soluble Dexamethason (Schering) to corneal tissue is quantitatively determined. Incorporation of radioactive sulfate into the glycosaminoglycanes and the sulfate-hexosamine-quotient of keratansulfate and chondroitinsulfate is pointed out. 1. A 2 times 10-7 M Dexamethason-concentration and 6 hours incubation time causes reducing of sulfate incorporation in the case of keratansulfate (I) about 94 per cent and chondroitinsulfate (II) about 89 percent compared to nontreated control. Increasing concentration of Dexamethason leads the inhibition of sulfate incorporation to 66 per cent (I) and 63 per cent (II) respectively. Removing the epithelium from the stroma before incubation the Dexamethason concentration and 6 h incubation time only 84 percent and 80 per cent radioactive sulfate is found in keratansulfate and chondroitinsulfate. An increasing Dexamethason concentration leads to inhibition of 55 per cent (I) and 46 per cent (II) respectively. 2. A comparable inhibition of sulfate incorporation is demonstrated with constant Dexamethason concentration and variable duration of incubation. A 1 hour incubation reduced incorporation of sulfate to 89 per cent for keratansulfate and 81 per cent for chondroitinsulfate. The 24 hours incubation inhibits incorporation to 69 per cent (I) and 65 per cent (II) respectively."}
{"id": "PMID:1078952", "title": "Electrical injuries to the eye.", "content": "The effect of electric current on the eyes of experimental animals was studied. An apparatus was constructed to allow single and multiple shock exposures from 0 to 600 volts at variable exposure times. The current was measured on a Tectronex Storage Oscilloscope. Mature Dutch rabbits were used as experimental animals. When rabbit eyes were subjected to a current of 500 volts for no less than 250 milliseconds and 400 milliamperes or more, a permanent decrease in the amplitude of the b-wave of the Electroretinogram resulted in some animals. No histological or ophthalmological alterations in the retina, optic nerve or vasculature could be observed using conventional techniques. The cataractogenic properties of electric current were also studied. Most previous observations dealing with this subject utilized a multiple shock technique--something which is unlikely to occur in any accidental exposure. This study focussed on the production of lens changes in rabbit eyes following single exposures of measured electric current. The biomicroscopic characteristics of these changes were described. Minimal lens changes were produced by single shock exposures ranging from 6 to 26 watts per second while typical electrical anterior subcapsular cataracts were produced by current of 23 to 80 watts per second. Exposure times were mostly 250 milliseconds or less since the survival rate of animals subjected to longer exposures made such studies unfeasible. Three patients with electrical injuries were presented and some of the pertinent literature was reviewed.", "contents": "Electrical injuries to the eye. The effect of electric current on the eyes of experimental animals was studied. An apparatus was constructed to allow single and multiple shock exposures from 0 to 600 volts at variable exposure times. The current was measured on a Tectronex Storage Oscilloscope. Mature Dutch rabbits were used as experimental animals. When rabbit eyes were subjected to a current of 500 volts for no less than 250 milliseconds and 400 milliamperes or more, a permanent decrease in the amplitude of the b-wave of the Electroretinogram resulted in some animals. No histological or ophthalmological alterations in the retina, optic nerve or vasculature could be observed using conventional techniques. The cataractogenic properties of electric current were also studied. Most previous observations dealing with this subject utilized a multiple shock technique--something which is unlikely to occur in any accidental exposure. This study focussed on the production of lens changes in rabbit eyes following single exposures of measured electric current. The biomicroscopic characteristics of these changes were described. Minimal lens changes were produced by single shock exposures ranging from 6 to 26 watts per second while typical electrical anterior subcapsular cataracts were produced by current of 23 to 80 watts per second. Exposure times were mostly 250 milliseconds or less since the survival rate of animals subjected to longer exposures made such studies unfeasible. Three patients with electrical injuries were presented and some of the pertinent literature was reviewed."}
{"id": "PMID:1078953", "title": "Black hypopyon: report of two cases.", "content": "Two patients are described in whom black hypopyon and black keratic precipitates occurred as a result of intraocular melanoma. In one instance the hypopyon was due to a clinically unsuspected primary choroidal melanoma in an eye with opaque media; in the second case the tumor was metastatic to the uvea froma cutaneous melanoma. Histological examination showed the hypopyon to be composed of pigment-laden macrophages and neoplastic melanoma cells.", "contents": "Black hypopyon: report of two cases. Two patients are described in whom black hypopyon and black keratic precipitates occurred as a result of intraocular melanoma. In one instance the hypopyon was due to a clinically unsuspected primary choroidal melanoma in an eye with opaque media; in the second case the tumor was metastatic to the uvea froma cutaneous melanoma. Histological examination showed the hypopyon to be composed of pigment-laden macrophages and neoplastic melanoma cells."}
{"id": "PMID:1078954", "title": "Cholesterolosis of the anterior chamber.", "content": "The clinicopathologic findings in eight eyes with cholesterolosis of the anterior chamber are presented.  In five patients the cholesterol was present as an anterior chamber deposit or free-floating crystals, or both, and was recognized clinically.  In two patients the anterior chamber cholesterol had incited a granulomatous inflammatory reaction and was mistaken clinically for lipid keratopathy.  Anterior chamber crystals were an incidental laboratory finding in the final patient.  Intraocular cholesterol may be present as free crystals or contained within foreign body granulomatous inflammation, macrophages, or fibrous tissue.  Possible sources of anterior chamber cholesterol crystals are discussed.  In most cases they seem to be derived from the breakdown of intraocular hemorrhage.", "contents": "Cholesterolosis of the anterior chamber. The clinicopathologic findings in eight eyes with cholesterolosis of the anterior chamber are presented.  In five patients the cholesterol was present as an anterior chamber deposit or free-floating crystals, or both, and was recognized clinically.  In two patients the anterior chamber cholesterol had incited a granulomatous inflammatory reaction and was mistaken clinically for lipid keratopathy.  Anterior chamber crystals were an incidental laboratory finding in the final patient.  Intraocular cholesterol may be present as free crystals or contained within foreign body granulomatous inflammation, macrophages, or fibrous tissue.  Possible sources of anterior chamber cholesterol crystals are discussed.  In most cases they seem to be derived from the breakdown of intraocular hemorrhage."}
{"id": "PMID:1078955", "title": "Goniotrepanation (gotrep) and further observations on this operation for chronic glaucoma.", "content": "The conclusions from the current data on the goniotrepanation, which are based upon the systematic observations on 175 eyes operated on with this microsurgical procedure and for a period of 2 to 4 years, can be characterized as follows: a) Intraocular pressure regulation obtained in 91 per cent of the cases.  b) Avoiding excessive hypotony.  Mean intraocular pressure value postoperatively 12 to 14 mm Hg.  c) Achieving a regular level filtration site overlying the trephination hole, limiting polycystic conjunctival degeneration to 8.5 per cent, which is common with the usual filtration procedures.  d) Maintenance of intact visual function in a great percentage of the operated eyes, with a decrease in visual acuity in 5 per cent without any observable visual deterioration.  e) The advantages of goniotrepanation listed above, emanating from observations over a 2-year period, point explicitly to the effectiveness of this operative procedure and explain the survival of goniotrepanation up to the present.", "contents": "Goniotrepanation (gotrep) and further observations on this operation for chronic glaucoma. The conclusions from the current data on the goniotrepanation, which are based upon the systematic observations on 175 eyes operated on with this microsurgical procedure and for a period of 2 to 4 years, can be characterized as follows: a) Intraocular pressure regulation obtained in 91 per cent of the cases.  b) Avoiding excessive hypotony.  Mean intraocular pressure value postoperatively 12 to 14 mm Hg.  c) Achieving a regular level filtration site overlying the trephination hole, limiting polycystic conjunctival degeneration to 8.5 per cent, which is common with the usual filtration procedures.  d) Maintenance of intact visual function in a great percentage of the operated eyes, with a decrease in visual acuity in 5 per cent without any observable visual deterioration.  e) The advantages of goniotrepanation listed above, emanating from observations over a 2-year period, point explicitly to the effectiveness of this operative procedure and explain the survival of goniotrepanation up to the present."}
{"id": "PMID:1078956", "title": "The screening of the central visual field.", "content": "The Armaly technique, a method of screening for glaucomatous field defects was evaluated in 100 normal eyes and 38 selected central field defects using the Goldmann perimeter and the Auto-Plot tangent screen. The screening results obtained from the Goldmann perimeter and the tangent screen using the selective perimetry of Armaly were compared with the results obtained from the Friedmann central field analyser.  Testing normal eyes we found the highest initial false positive rate with the Friedmann analyser and the lowest initial false positive rate with the Auto-Plot tangent screen.  The specificity of the Friedmann analyser for detecting early central field changes is less than the Goldmann perimeter or the Auto-Plot tangent screen when using Armaly's technique.  On the other hand, the Friedmann analyser turned out to be the easiest instrument to handle and requires least experiences for the observer.  Some problems of mass screening perimetry are discussed.", "contents": "The screening of the central visual field. The Armaly technique, a method of screening for glaucomatous field defects was evaluated in 100 normal eyes and 38 selected central field defects using the Goldmann perimeter and the Auto-Plot tangent screen. The screening results obtained from the Goldmann perimeter and the tangent screen using the selective perimetry of Armaly were compared with the results obtained from the Friedmann central field analyser.  Testing normal eyes we found the highest initial false positive rate with the Friedmann analyser and the lowest initial false positive rate with the Auto-Plot tangent screen.  The specificity of the Friedmann analyser for detecting early central field changes is less than the Goldmann perimeter or the Auto-Plot tangent screen when using Armaly's technique.  On the other hand, the Friedmann analyser turned out to be the easiest instrument to handle and requires least experiences for the observer.  Some problems of mass screening perimetry are discussed."}
{"id": "PMID:1078957", "title": "[Herpes Simplex Keratitis under the Scanning lectron Microscope(author's transl)].", "content": "In 12 rabbits a dendritic keratitis could be provoked by applying a suspension of herpes viruses (Herpes virus hominis -- HVH type 1) onto their previously both uviolised and non-uviolised corneae. 4 days later ophthalmectomy was performed and the corneae were examined by the scanning electron microscope. Macroscopically, both in the uviolised eyes and the control eyes punctiform lesions of the corneae were observed. After short ray-treatment (3 min) no differences were present between the uviolised and non-uviolised eyes. At longer time of exposure (7--15 min), however, a more distinct alteration occurred in the uviolised eye. The scanning-electron-microscopical picture showed the dendritic lesions to be composed of numerous circular foci of 100 to 200 mu. There were no differences in the lesions of the uviolised and non-uviolised corneae. The fact that after ultraviolet ray-treatment a great part of the epithelial cells is sloughed off, but, nevertheless, the same dendritic lesions develop, contradicts the assumption of a morphological substrate of the epithelial cells being the inducing factor.", "contents": "[Herpes Simplex Keratitis under the Scanning lectron Microscope(author's transl)]. In 12 rabbits a dendritic keratitis could be provoked by applying a suspension of herpes viruses (Herpes virus hominis -- HVH type 1) onto their previously both uviolised and non-uviolised corneae. 4 days later ophthalmectomy was performed and the corneae were examined by the scanning electron microscope. Macroscopically, both in the uviolised eyes and the control eyes punctiform lesions of the corneae were observed. After short ray-treatment (3 min) no differences were present between the uviolised and non-uviolised eyes. At longer time of exposure (7--15 min), however, a more distinct alteration occurred in the uviolised eye. The scanning-electron-microscopical picture showed the dendritic lesions to be composed of numerous circular foci of 100 to 200 mu. There were no differences in the lesions of the uviolised and non-uviolised corneae. The fact that after ultraviolet ray-treatment a great part of the epithelial cells is sloughed off, but, nevertheless, the same dendritic lesions develop, contradicts the assumption of a morphological substrate of the epithelial cells being the inducing factor."}
{"id": "PMID:1078958", "title": "[Ultrastructural studies on the pterygium. I. Epithelium and glands of the conjunctival part (author's transl)].", "content": "13 pterygia from patients aged between 39 and 81 years are studied by electron microscopy. The pterygium is covered by a multilayered, highly differentiated cylindrocellular epithelium. Arrangement and height of the microvilli of the superficial epithelial cells differ from those of the conjunctival epithelium. Morphological signs of secretion and resorption are found. As a characteristic the basal epithelial cells of the pterygium are rich in metaplasmatic filaments. Tubular glands are localised underneath the covering epithelium; they may reach the deep substantia propria. Their walls consist of a single layer of cylindrical cells. A varying number of goblet-cells are embedded between them. The secretion by the epithelial formations of the pterygium is of the merocrine, holocrine and microapocrine type.", "contents": "[Ultrastructural studies on the pterygium. I. Epithelium and glands of the conjunctival part (author's transl)]. 13 pterygia from patients aged between 39 and 81 years are studied by electron microscopy. The pterygium is covered by a multilayered, highly differentiated cylindrocellular epithelium. Arrangement and height of the microvilli of the superficial epithelial cells differ from those of the conjunctival epithelium. Morphological signs of secretion and resorption are found. As a characteristic the basal epithelial cells of the pterygium are rich in metaplasmatic filaments. Tubular glands are localised underneath the covering epithelium; they may reach the deep substantia propria. Their walls consist of a single layer of cylindrical cells. A varying number of goblet-cells are embedded between them. The secretion by the epithelial formations of the pterygium is of the merocrine, holocrine and microapocrine type."}
{"id": "PMID:1078959", "title": "[Ultrastructural studies on the pterygium. II. Connective tissue, vessels and nerves of the conjunctival part (author's transl)].", "content": "The stromal part of the pterygium is remarkably rich in plasma cells. The connective intercellular substance shows two different pathological modifications: A clotting together of collagen fibres to broad, osmiophilic, elastoid bands and a widening of the spaces containing the granular nonfibrillar ground-substance . The vascular system consists mostly of fenestrated sinusoid capillaries. Their endothelial cells frequently show degenerative changes and the basement membrane of the vessels can be as thick as up to 50--100 fold the normal size. For the first time nervous formations could be found in the pterygium. Axons of the autonomic system join the basal epithelial cells, where they form terminal endings. Encapsulated myelinated and nonmyelinated corpusles probably stem from the sensory nervous system.", "contents": "[Ultrastructural studies on the pterygium. II. Connective tissue, vessels and nerves of the conjunctival part (author's transl)]. The stromal part of the pterygium is remarkably rich in plasma cells. The connective intercellular substance shows two different pathological modifications: A clotting together of collagen fibres to broad, osmiophilic, elastoid bands and a widening of the spaces containing the granular nonfibrillar ground-substance . The vascular system consists mostly of fenestrated sinusoid capillaries. Their endothelial cells frequently show degenerative changes and the basement membrane of the vessels can be as thick as up to 50--100 fold the normal size. For the first time nervous formations could be found in the pterygium. Axons of the autonomic system join the basal epithelial cells, where they form terminal endings. Encapsulated myelinated and nonmyelinated corpusles probably stem from the sensory nervous system."}
{"id": "PMID:1078960", "title": "[Investigations on the effectiveness of a new antimycotic through experimental keratomycosis in rabbits (author's transl)].", "content": "Thirty-two rabbits infected with Fumigatus keratitis were treated for a period of  10 to 20 days with a new antifungal agent known as \"BAY b 5097\" (Canesten). It was administered to 6 animals (500 mg/kg body weight; 12 eyes) orally; 20 received unilateral topical treatment. In both groups the therapy failed to supress mycosis in the avascular cornea. In topical treatment problems concerning common solvents arise: They are unable to reach the intracorneal infection, and most of them are incompatible for conjunctival use.", "contents": "[Investigations on the effectiveness of a new antimycotic through experimental keratomycosis in rabbits (author's transl)]. Thirty-two rabbits infected with Fumigatus keratitis were treated for a period of  10 to 20 days with a new antifungal agent known as \"BAY b 5097\" (Canesten). It was administered to 6 animals (500 mg/kg body weight; 12 eyes) orally; 20 received unilateral topical treatment. In both groups the therapy failed to supress mycosis in the avascular cornea. In topical treatment problems concerning common solvents arise: They are unable to reach the intracorneal infection, and most of them are incompatible for conjunctival use."}
{"id": "PMID:1078961", "title": "[Standard candida mycosis in the rabbit eye (author's transl)].", "content": "Whole-body X-irradiation administered to rabbits prior to intracorneal inoculation with Candida albicans is required to produce a verifiable mycotic keratitis. Damage to the corneal tissue, which in rabbits is generally resistant to Candida, can thus be avoided. The mycosis usually lasts for a period of 12 to 14 days. Twenty-four animals (48 eyes) were infected. The fungi were recultivated in 21 animals (24 eyes). All attempts were successful.", "contents": "[Standard candida mycosis in the rabbit eye (author's transl)]. Whole-body X-irradiation administered to rabbits prior to intracorneal inoculation with Candida albicans is required to produce a verifiable mycotic keratitis. Damage to the corneal tissue, which in rabbits is generally resistant to Candida, can thus be avoided. The mycosis usually lasts for a period of 12 to 14 days. Twenty-four animals (48 eyes) were infected. The fungi were recultivated in 21 animals (24 eyes). All attempts were successful."}
{"id": "PMID:1078962", "title": "[The unilateral emigration of leukocytes from conjunctival vessels (author's transl)].", "content": "The phenomenon of unilateral leukodiapedesis is demonstrated in the irritated conjunctiva of rabbits and rhesus-monkeys. The leukocytes will penetrate the vessel walls first in the neighbourhood of the irritation. This can be demonstrated during the first minutes after the onset of an inflammatory reaction only, i.e. prior to a general leukocytosis of the tissue. By fixation with glutaraldehyde or osmium-tetroxyde the dynamic movement can be visualized and the directions of the irritation and of the migration are obvious in the conjunctiva. So this tissue provides an excellent model to demonstrate the unilateral sticking and the directed migration of leukocytes through vessel walls, a fact to which references seem to be missing in the ophthalmopathologic and pathologic literature.", "contents": "[The unilateral emigration of leukocytes from conjunctival vessels (author's transl)]. The phenomenon of unilateral leukodiapedesis is demonstrated in the irritated conjunctiva of rabbits and rhesus-monkeys. The leukocytes will penetrate the vessel walls first in the neighbourhood of the irritation. This can be demonstrated during the first minutes after the onset of an inflammatory reaction only, i.e. prior to a general leukocytosis of the tissue. By fixation with glutaraldehyde or osmium-tetroxyde the dynamic movement can be visualized and the directions of the irritation and of the migration are obvious in the conjunctiva. So this tissue provides an excellent model to demonstrate the unilateral sticking and the directed migration of leukocytes through vessel walls, a fact to which references seem to be missing in the ophthalmopathologic and pathologic literature."}
{"id": "PMID:1078963", "title": "DC recording of the human corneofundal potential.", "content": "The limitation of an AC technique for the amplification and display of the indirectly recorded human corneofundal potential (CFP)is discussed. A Mingograph M34 with EMT 12 B preamplifiers (Siemens) has  been tested with regard to a DC recording of the CFP. With the same purpose the electrical characteristics of two electrode types--a Kaiser lead alloy electrode and a Beckman silver-silver chloride cupped electrode--were examined and a suitable preparation of the skin contact area indicated. Finally the base line stability was evaluated and a comparative study of AC and DC recordings of the CFP presented and subjected to statistical anaylsis.", "contents": "DC recording of the human corneofundal potential. The limitation of an AC technique for the amplification and display of the indirectly recorded human corneofundal potential (CFP)is discussed. A Mingograph M34 with EMT 12 B preamplifiers (Siemens) has  been tested with regard to a DC recording of the CFP. With the same purpose the electrical characteristics of two electrode types--a Kaiser lead alloy electrode and a Beckman silver-silver chloride cupped electrode--were examined and a suitable preparation of the skin contact area indicated. Finally the base line stability was evaluated and a comparative study of AC and DC recordings of the CFP presented and subjected to statistical anaylsis."}
{"id": "PMID:1078964", "title": "Investigations on laser coagulated rat eyes by fluorescence angiography and microscopy.", "content": "Laser coagulation using 50 and 100 mW of power, a spot of 50 microns and an exposure time of 0, 2 seconds were performed on rat eye fundi. We observed the effects which were produced in the retina and choroid utilizing both fluorescence photomicroscopy with incident excitation light and fluorescence angiography. No distinct differences were noticed between the effects produced by 50 and by 100 mW. Both energies damaged choroid and retina, especially the outer part of the retina. Moreover, fluorescein angiography within 24 hours gave us effective information for estimating the applied laser effect.", "contents": "Investigations on laser coagulated rat eyes by fluorescence angiography and microscopy. Laser coagulation using 50 and 100 mW of power, a spot of 50 microns and an exposure time of 0, 2 seconds were performed on rat eye fundi. We observed the effects which were produced in the retina and choroid utilizing both fluorescence photomicroscopy with incident excitation light and fluorescence angiography. No distinct differences were noticed between the effects produced by 50 and by 100 mW. Both energies damaged choroid and retina, especially the outer part of the retina. Moreover, fluorescein angiography within 24 hours gave us effective information for estimating the applied laser effect."}
{"id": "PMID:1078965", "title": "Alkali and acid burns of the rabbit eye: measurement of aqueous flow by means of intravenously injected Na-125 I and Na-131I.", "content": "Rabbit eyes were burned either with 1N NaOH or 1 N HC1. On the fifth day following the injury, aqueous humour flow was determined according to a method originally described by Becker (1962), using intravenous injection of Na-125I and Na-131I in different time intervals. In alkali-burned eyes the main finding was stoppage of the aqueous humour flow, probably due to obstruction of outflow channels. In acid-burned eyes the significantly elevated diffusion out of the eye was probably caused by altered function of the highly irritated and hyperemic iris. Results confirmed the previous findings achieved by the rate of disappearence of 14-C-glucose injected into the anterior chamber of the burned eyes.", "contents": "Alkali and acid burns of the rabbit eye: measurement of aqueous flow by means of intravenously injected Na-125 I and Na-131I. Rabbit eyes were burned either with 1N NaOH or 1 N HC1. On the fifth day following the injury, aqueous humour flow was determined according to a method originally described by Becker (1962), using intravenous injection of Na-125I and Na-131I in different time intervals. In alkali-burned eyes the main finding was stoppage of the aqueous humour flow, probably due to obstruction of outflow channels. In acid-burned eyes the significantly elevated diffusion out of the eye was probably caused by altered function of the highly irritated and hyperemic iris. Results confirmed the previous findings achieved by the rate of disappearence of 14-C-glucose injected into the anterior chamber of the burned eyes."}
{"id": "PMID:1078966", "title": "[Glucose and lactate concentration in tears of rabbits following mechanical stress and wearing of contact lenses].", "content": "The concentration of glucose and lactat in tears is significantly different after slight or strong irritation of the eye. More glucose and lactat can be washed out of alterated tissue compared to normal one, although we estimate that the metabolits are primary more secreted after irritation. Under soft contact-lenses the concentration of both metabolits increase continiously during a certain period. Under hard contact-lenses the single measurements are very different. We have to estimate many disturbing factors.", "contents": "[Glucose and lactate concentration in tears of rabbits following mechanical stress and wearing of contact lenses]. The concentration of glucose and lactat in tears is significantly different after slight or strong irritation of the eye. More glucose and lactat can be washed out of alterated tissue compared to normal one, although we estimate that the metabolits are primary more secreted after irritation. Under soft contact-lenses the concentration of both metabolits increase continiously during a certain period. Under hard contact-lenses the single measurements are very different. We have to estimate many disturbing factors."}
{"id": "PMID:1078967", "title": "[Ultrasonic biometry and oculopression].", "content": "The changes of the intraocular distances provoked by oculopression (10 min, 40 mm HG) were explored by ultrasonic biometry. Immediately after removing the oculopressor, a flattening of the anterior chamber and a deepening of the vitreous cavity occurred. The maximal deepening of the anterior chamber together with the shortening of the vitreous axis appeared 9 min after oculopression on an average. The causes of the predominance of the initial lengthening (0.24 mm on an average) and of the following shortening (0.16 mm on an average) of the vitreous cavity compared with contrary changes of the anterior chamber are discussed.", "contents": "[Ultrasonic biometry and oculopression]. The changes of the intraocular distances provoked by oculopression (10 min, 40 mm HG) were explored by ultrasonic biometry. Immediately after removing the oculopressor, a flattening of the anterior chamber and a deepening of the vitreous cavity occurred. The maximal deepening of the anterior chamber together with the shortening of the vitreous axis appeared 9 min after oculopression on an average. The causes of the predominance of the initial lengthening (0.24 mm on an average) and of the following shortening (0.16 mm on an average) of the vitreous cavity compared with contrary changes of the anterior chamber are discussed."}
{"id": "PMID:1078969", "title": "Tumors of the small intestine.", "content": "An analysis of 80 cases of malignant and benign tumors of the small intestine is presented, and major clinical features of various histologic lesions are discussed. Tumors of the small intestine are rare, and the small bowel may have intrinsic protective systems against the development of neoplasms. Gastrointestinal bleeding and symptoms of intermittent obstruction are prominent findings in patients with symptomatic benign tumors, although many benign tumors are incidental findings at operation for an unrelated disease. Benign lesions should be removed by local excision. Virtually all malignant tumors are symptomatic with abdominal pain, nausea and vomiting, anorexia and weight loss, and gastrointestinal bleeding being common findings. Earlier diagnosis is essential if the prognosis for patients with small intestinal malignancies is to be improved. A high degree of suspicion must be aroused with any vague, nonspecific gastrointestinal complaints in patients over 40 years of age. Exploratory celiotomy should be performed in symptomatic patients even though no abnormality may be detected on roentgenographic examination.", "contents": "Tumors of the small intestine. An analysis of 80 cases of malignant and benign tumors of the small intestine is presented, and major clinical features of various histologic lesions are discussed. Tumors of the small intestine are rare, and the small bowel may have intrinsic protective systems against the development of neoplasms. Gastrointestinal bleeding and symptoms of intermittent obstruction are prominent findings in patients with symptomatic benign tumors, although many benign tumors are incidental findings at operation for an unrelated disease. Benign lesions should be removed by local excision. Virtually all malignant tumors are symptomatic with abdominal pain, nausea and vomiting, anorexia and weight loss, and gastrointestinal bleeding being common findings. Earlier diagnosis is essential if the prognosis for patients with small intestinal malignancies is to be improved. A high degree of suspicion must be aroused with any vague, nonspecific gastrointestinal complaints in patients over 40 years of age. Exploratory celiotomy should be performed in symptomatic patients even though no abnormality may be detected on roentgenographic examination."}
{"id": "PMID:1078970", "title": "Suppression of T-lymphocyte rosettes by rifampin. Studies in normals and patients with tuberculosis.", "content": "Studies of circulating T- and B-lymphocyte rosettes were done in 20 healthy controls and 29 patients with pulmonary tuberculosis, including 18 receiving rifampin chemotherapy. Eight of the 18 patients in the rifampin group (44%) had significant suppression of their T-cell rosettes as compared with patients with tuberculosis not receiving rifampin (P less than 0.005). Seven of the eight patients with suppressed T-cell rosettes had received the drug for longer than 6 weeks. No significant difference was observed in circulating T- and B-lymphocyte rosettes between healthy controls and patients not receiving rifampin. Administration of this drug to two healthy subjects in a dosage of 600 mg daily for 28 days was associated with suppression of T-lymphocyte rosettes. The maximum decreases of 40% and 37% were observed at 14 and 21 days respectively, with return to base-line values within 2 weeks of discontinuation of drug therapy; this indicates complete reversibility. Long-term sequential studies will be required to determine whether T-cell suppression after prolonged therapy with rifampin is also reversible or associated with any harmful sequels.", "contents": "Suppression of T-lymphocyte rosettes by rifampin. Studies in normals and patients with tuberculosis. Studies of circulating T- and B-lymphocyte rosettes were done in 20 healthy controls and 29 patients with pulmonary tuberculosis, including 18 receiving rifampin chemotherapy. Eight of the 18 patients in the rifampin group (44%) had significant suppression of their T-cell rosettes as compared with patients with tuberculosis not receiving rifampin (P less than 0.005). Seven of the eight patients with suppressed T-cell rosettes had received the drug for longer than 6 weeks. No significant difference was observed in circulating T- and B-lymphocyte rosettes between healthy controls and patients not receiving rifampin. Administration of this drug to two healthy subjects in a dosage of 600 mg daily for 28 days was associated with suppression of T-lymphocyte rosettes. The maximum decreases of 40% and 37% were observed at 14 and 21 days respectively, with return to base-line values within 2 weeks of discontinuation of drug therapy; this indicates complete reversibility. Long-term sequential studies will be required to determine whether T-cell suppression after prolonged therapy with rifampin is also reversible or associated with any harmful sequels."}
{"id": "PMID:1078975", "title": "Clinicopathologic case report: ultrastructure of the epithelial dystrophy of Meesmann.", "content": "As in previous studies of Meesmann dystrophy, intense vacuolation of the epithelium was observed. However, neither histochemical nor electron microscopical examination of the specimens showed any evidence of abnormal quantities of glycogen or the electron dense peculiar substance of Kuwabara and Ciccarelli. Electron microscopy indicated that the basic alteration leading to the manifestations of the dystrophy was electron dense bodies in the cytoplasm of the basal epithelial cells. These electron dense bodies appeared similar to lysosomes. In the more superficial cell layers, the electron dense bodies were engulfed by vacuoles that gradually increased in number and size to fill the cytoplasm and, finally, destroyed the most superficial cells.", "contents": "Clinicopathologic case report: ultrastructure of the epithelial dystrophy of Meesmann. As in previous studies of Meesmann dystrophy, intense vacuolation of the epithelium was observed. However, neither histochemical nor electron microscopical examination of the specimens showed any evidence of abnormal quantities of glycogen or the electron dense peculiar substance of Kuwabara and Ciccarelli. Electron microscopy indicated that the basic alteration leading to the manifestations of the dystrophy was electron dense bodies in the cytoplasm of the basal epithelial cells. These electron dense bodies appeared similar to lysosomes. In the more superficial cell layers, the electron dense bodies were engulfed by vacuoles that gradually increased in number and size to fill the cytoplasm and, finally, destroyed the most superficial cells."}
{"id": "PMID:1078976", "title": "Hearing loss after head injury.", "content": "This work reports on the hearing loss of 395 patients who were hospitalized after brain concussion. The percentage of hearing loss and vestibular disturbances appearing after fracture of the temporal bone is higher than in cases with skull fractures without fracture of the temporal bone or with brain concussion alone. Conductive deafness caused by head injury usually disappears in two months time. If conductive deafness remains, the suspicion of dislocation of the ossicular bones arises and such patients must undergo an operation. As the cases where sensorineural deafness disappeared within six months after head injury were rare, final evaluation of the hearing loss can be made one year after the head injury. In the majority of cases, vestibular disturbances and positional nystagmus disappear within six months after the head injury.", "contents": "Hearing loss after head injury. This work reports on the hearing loss of 395 patients who were hospitalized after brain concussion. The percentage of hearing loss and vestibular disturbances appearing after fracture of the temporal bone is higher than in cases with skull fractures without fracture of the temporal bone or with brain concussion alone. Conductive deafness caused by head injury usually disappears in two months time. If conductive deafness remains, the suspicion of dislocation of the ossicular bones arises and such patients must undergo an operation. As the cases where sensorineural deafness disappeared within six months after head injury were rare, final evaluation of the hearing loss can be made one year after the head injury. In the majority of cases, vestibular disturbances and positional nystagmus disappear within six months after the head injury."}
{"id": "PMID:1078977", "title": "Acute severe cardiac failure complicating myocardial infarction. Experience with 100 patients referred for consideration of mechanical left ventricular assistance.", "content": "One hundred patients were referred with suspected acute cardiac failure following acute myocardial infarction. The diagnosis was confirmed in 72: 31 of these patients underwent elective medical treatment, with 2 survivors (6%); 41 were accepted for counter pulsation, but 9 died before this could be initiated and another 2 died shortly after vain attempts to pass the balloon catheter were abandoned; 30 patients underwent counterpulsation with 14 hospital survivors (47%). Survivor status was usually good. Results of counter pulsation were better in patients who were not shocked (with 5/5 survivors) than in those who were in shock (with 9 of 25 survivors). Results support the view that counterpulsation (alone or combined with corrective surgery) may play an important role in the complications of myocardial infarction provided intervention is early.", "contents": "Acute severe cardiac failure complicating myocardial infarction. Experience with 100 patients referred for consideration of mechanical left ventricular assistance. One hundred patients were referred with suspected acute cardiac failure following acute myocardial infarction. The diagnosis was confirmed in 72: 31 of these patients underwent elective medical treatment, with 2 survivors (6%); 41 were accepted for counter pulsation, but 9 died before this could be initiated and another 2 died shortly after vain attempts to pass the balloon catheter were abandoned; 30 patients underwent counterpulsation with 14 hospital survivors (47%). Survivor status was usually good. Results of counter pulsation were better in patients who were not shocked (with 5/5 survivors) than in those who were in shock (with 9 of 25 survivors). Results support the view that counterpulsation (alone or combined with corrective surgery) may play an important role in the complications of myocardial infarction provided intervention is early."}
{"id": "PMID:1078978", "title": "Isolation and identification of 1,25-dihydroxyvitamin D2.", "content": "The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat.", "contents": "Isolation and identification of 1,25-dihydroxyvitamin D2. The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat."}
{"id": "PMID:1078979", "title": "Net Clminus flux in short-circuited skin of Rana pipiens: ouabain sensitivity and Na+ +K+ dependence.", "content": "Skin of Rama pipiens, like many other species, is considered to actively transport only Na+ when bathed in Ringer's solution on both sides. However, net Clminus influx was previously described by us in short-circuited skin of Rana pipiens, in the summer season. The skins were of low PD (5-30 mV) and high Clminus conductance. Comparison of these findings with other series indicated inverse seasonal variation between PD and Clminus conductance. It was postulated that active Clminus transport exists at all Clminus -conductance levels but at higher PD is too small to be easily detected. This report evaluates Clminus transport across skins of higher PD (and lower conductance) in winter and further characterizes the system. Net Clminus influx was demonstrated over a wide PD range under short-circuit conditions and was inversely related to the magnitude of the open-circuit PD. It was inhibited by ouabain and dependent upon Na+ in the outside medium and K+ in the inside medium. It is concluded that this is the same system present in skin of Leptodactylus ocellatus and may not he unique to these two species. It appears to be distinctly different from the acetazolamide-sensitive influx system seen in \"low-Clminus\" solutions in skins of numerous species. The findings are discussed in terms of (1) parallel Na+ and Clminus active transport mechanisms and (2) a neutral NaCl-pump model, recently proposed by Rehm.", "contents": "Net Clminus flux in short-circuited skin of Rana pipiens: ouabain sensitivity and Na+ +K+ dependence. Skin of Rama pipiens, like many other species, is considered to actively transport only Na+ when bathed in Ringer's solution on both sides. However, net Clminus influx was previously described by us in short-circuited skin of Rana pipiens, in the summer season. The skins were of low PD (5-30 mV) and high Clminus conductance. Comparison of these findings with other series indicated inverse seasonal variation between PD and Clminus conductance. It was postulated that active Clminus transport exists at all Clminus -conductance levels but at higher PD is too small to be easily detected. This report evaluates Clminus transport across skins of higher PD (and lower conductance) in winter and further characterizes the system. Net Clminus influx was demonstrated over a wide PD range under short-circuit conditions and was inversely related to the magnitude of the open-circuit PD. It was inhibited by ouabain and dependent upon Na+ in the outside medium and K+ in the inside medium. It is concluded that this is the same system present in skin of Leptodactylus ocellatus and may not he unique to these two species. It appears to be distinctly different from the acetazolamide-sensitive influx system seen in \"low-Clminus\" solutions in skins of numerous species. The findings are discussed in terms of (1) parallel Na+ and Clminus active transport mechanisms and (2) a neutral NaCl-pump model, recently proposed by Rehm."}
{"id": "PMID:1078980", "title": "Dipole moment, enthalpy, and entropy changes of Hodgkin-Huxley type kinetic units.", "content": "Dipole moment, enthalpy, and entropy changes were calculated for hypothetical structural units which control the opening and closing of ionic channels in axon membranes. The changes of these thermodynamic functions were calculated both for activation (transition to intermediate complex) and for the structural transformation as a whole. The calculations are based on the experimentally determined Q10 values and the empirical formulae for the rate constants (alpha's and beta's) as functions of membrane potentials in Hodgkin-Huxley type models. From the calculated thermodynamic functions we suggest that the specific structural units of the axon membranes are probably of macromolecular (possible protein-like) dimensions with large dipole moments (hundreds of debyes). The calculated dipole moment changes of a single structural unit indicate that in many cases these dipole moments saturate at strong depolarizations or hyperpolarizations. The transitions in structural units show substantial activation enthalpies and entropies but the net enthalpy and entropy changes are practically negligible for the transition as a whole, i.e. the structural units presumably undergo displacements. While the calculated dipole moment changes associated with structural transformations in Loligo and Myxicola show similar potential dependencies, those for Rana usually show a different behavior. The relevance of the dipole moment changes to gating currents is discussed.", "contents": "Dipole moment, enthalpy, and entropy changes of Hodgkin-Huxley type kinetic units. Dipole moment, enthalpy, and entropy changes were calculated for hypothetical structural units which control the opening and closing of ionic channels in axon membranes. The changes of these thermodynamic functions were calculated both for activation (transition to intermediate complex) and for the structural transformation as a whole. The calculations are based on the experimentally determined Q10 values and the empirical formulae for the rate constants (alpha's and beta's) as functions of membrane potentials in Hodgkin-Huxley type models. From the calculated thermodynamic functions we suggest that the specific structural units of the axon membranes are probably of macromolecular (possible protein-like) dimensions with large dipole moments (hundreds of debyes). The calculated dipole moment changes of a single structural unit indicate that in many cases these dipole moments saturate at strong depolarizations or hyperpolarizations. The transitions in structural units show substantial activation enthalpies and entropies but the net enthalpy and entropy changes are practically negligible for the transition as a whole, i.e. the structural units presumably undergo displacements. While the calculated dipole moment changes associated with structural transformations in Loligo and Myxicola show similar potential dependencies, those for Rana usually show a different behavior. The relevance of the dipole moment changes to gating currents is discussed."}
{"id": "PMID:1078981", "title": "Further studies of canine von Willebrand's disease.", "content": "Additional characterization of von Willebrand's disease (VWD) in a family of German shepherd dogs is presented. Genetic studies of three generations of affected dogs indicate that about 50% of the progeny are affected if one parent has VWD and about 60% if both parents have the defect. Some of these progeny manifested an incomplete form of VWD, suggesting autosomal dominant inheritance with variable expressivity. The disease become progressively less severe with advancing age and repeated pregnancies. Ristocetin-induced platelet aggregation was significantly reduced in VWD dogs as compared with normal, thrombopathic, and hemophilic carrier dogs. Immunodiffusion and electroimmunodiffusion studies with rabbit anticanine factor VII showed the level of factor VII-related antigen to be low in VWD dogs but present in increased amounts in hemophilic dogs. VWD affected dogs had markedly delayed hemostatic plug formation, but their plugs appeared normal by light and electron microscopy. Their platelet nucleotides, ATP/ADP ration, and platelet protein content were normal. Platelet and fibrinogen survival times with [75Se] selenomethionine were also normal, although platelets from VWD dogs incorporated more radioactivity than did those from normal dogs or from dogs with incomplete VWD.", "contents": "Further studies of canine von Willebrand's disease. Additional characterization of von Willebrand's disease (VWD) in a family of German shepherd dogs is presented. Genetic studies of three generations of affected dogs indicate that about 50% of the progeny are affected if one parent has VWD and about 60% if both parents have the defect. Some of these progeny manifested an incomplete form of VWD, suggesting autosomal dominant inheritance with variable expressivity. The disease become progressively less severe with advancing age and repeated pregnancies. Ristocetin-induced platelet aggregation was significantly reduced in VWD dogs as compared with normal, thrombopathic, and hemophilic carrier dogs. Immunodiffusion and electroimmunodiffusion studies with rabbit anticanine factor VII showed the level of factor VII-related antigen to be low in VWD dogs but present in increased amounts in hemophilic dogs. VWD affected dogs had markedly delayed hemostatic plug formation, but their plugs appeared normal by light and electron microscopy. Their platelet nucleotides, ATP/ADP ration, and platelet protein content were normal. Platelet and fibrinogen survival times with [75Se] selenomethionine were also normal, although platelets from VWD dogs incorporated more radioactivity than did those from normal dogs or from dogs with incomplete VWD."}
{"id": "PMID:1078982", "title": "Pure red cell aplasia (PRCA): Response of three patients of cyclophosphamide and/or antilymphocyte globulin (ALG) and demonstration of two types of serum IgG inhibitors to erythropoiesis.", "content": "Three cases of adult pure red cell aplasia (PRCA) ARE REPORTED. All patients proved refractory to various combinations of androgens and corticosteroids. The first case, harboring a thymoma, showed a complete clinical remission following cyclophosphamide therapy. The second and third responded similarly to either a combined cyclophosphamide + antilymphocyte globulin (ALG) treatment or to ALG administration preceded by a small dosage of cyclophosphamide, which had proved ineffective when administered alone. Serum IgG inhibitors to erythropoiesis were demonstrated in all cases by  means of in vivo and/or in vitro techniques. The inhibitor(s), although directed against the erythroid marrow in both the first and third patients (PRCA type A), apparently functioned as an antibody to circulating erythropoientin (Ep) in the second case (PRCA type B). The inhibitor(s) was always absent in postremission samples. Additionally, experimental models for both types of human PRCA were established in normal rodents. The present studies support the contention that adult PRCA is an autoimmune disease. The therapeutic role of cytotoxic-immunodepressive agents in PRCA patients is confirmed. It is emphasized that ALG may represent an additional therapeutic tool in cases resistant to cyclophosphamide and/or steroids. In addition, cyclophosphamide proved effective in a patient harboring a thymoma not amenable to surgery. Finally, it is postulated that IgG serum autoantibodies, directed against either an early erythroid precursor (PRCA type A) or, more rarely, circulating Ep (PRCA type B), play a major role in the pathogenesis of the disease.", "contents": "Pure red cell aplasia (PRCA): Response of three patients of cyclophosphamide and/or antilymphocyte globulin (ALG) and demonstration of two types of serum IgG inhibitors to erythropoiesis. Three cases of adult pure red cell aplasia (PRCA) ARE REPORTED. All patients proved refractory to various combinations of androgens and corticosteroids. The first case, harboring a thymoma, showed a complete clinical remission following cyclophosphamide therapy. The second and third responded similarly to either a combined cyclophosphamide + antilymphocyte globulin (ALG) treatment or to ALG administration preceded by a small dosage of cyclophosphamide, which had proved ineffective when administered alone. Serum IgG inhibitors to erythropoiesis were demonstrated in all cases by  means of in vivo and/or in vitro techniques. The inhibitor(s), although directed against the erythroid marrow in both the first and third patients (PRCA type A), apparently functioned as an antibody to circulating erythropoientin (Ep) in the second case (PRCA type B). The inhibitor(s) was always absent in postremission samples. Additionally, experimental models for both types of human PRCA were established in normal rodents. The present studies support the contention that adult PRCA is an autoimmune disease. The therapeutic role of cytotoxic-immunodepressive agents in PRCA patients is confirmed. It is emphasized that ALG may represent an additional therapeutic tool in cases resistant to cyclophosphamide and/or steroids. In addition, cyclophosphamide proved effective in a patient harboring a thymoma not amenable to surgery. Finally, it is postulated that IgG serum autoantibodies, directed against either an early erythroid precursor (PRCA type A) or, more rarely, circulating Ep (PRCA type B), play a major role in the pathogenesis of the disease."}
{"id": "PMID:1078983", "title": "[Diagnosis and differential diagnosis of hairy cell leukemia].", "content": "On account of 2 own observations, main clinical and diagnostic features of Hairy cell leukemia (HCL) will be discussed. HCL is a rare, unusual type of chronic leukemia and is predominantly particular of middle-aged men. The occurrence of middle-sized lymphoid cells having a hairy cytoplasmic edge, and a tartrate-resistant acid PHOsphatase isoenzyme are the characteristic criteria of the HCL. The diffuse infiltration by hairy cells affecting primarily the spleen and bone marrow results in anaemia, granulocytopenia, thrombocytopenia and splenomegaly. Differential diagnosis have to be made in relation to other lymphatic leukemias, leukemic malignant lymphomas and monoclonal gammopathies as well as lymphotropic viral infections. Immunologic behaviour of hairy cells is like that of B-lymphocytes. Therefore, the term \"leukemic reticuloendotheliosis\" should no longer be applied.", "contents": "[Diagnosis and differential diagnosis of hairy cell leukemia]. On account of 2 own observations, main clinical and diagnostic features of Hairy cell leukemia (HCL) will be discussed. HCL is a rare, unusual type of chronic leukemia and is predominantly particular of middle-aged men. The occurrence of middle-sized lymphoid cells having a hairy cytoplasmic edge, and a tartrate-resistant acid PHOsphatase isoenzyme are the characteristic criteria of the HCL. The diffuse infiltration by hairy cells affecting primarily the spleen and bone marrow results in anaemia, granulocytopenia, thrombocytopenia and splenomegaly. Differential diagnosis have to be made in relation to other lymphatic leukemias, leukemic malignant lymphomas and monoclonal gammopathies as well as lymphotropic viral infections. Immunologic behaviour of hairy cells is like that of B-lymphocytes. Therefore, the term \"leukemic reticuloendotheliosis\" should no longer be applied."}
{"id": "PMID:1078984", "title": "Comparative study between endoscopy and radiology in acute upper gastrointestinal haemorrhage.", "content": "A total of 158 patients with acute upper gastrointestinal haemorrhage were studied, and the 53 patients on whom emergency endoscopies were performed were compared with the remaining 105. The cause of the bleeding was found in 51 of the endoscopy group and 39 of the control group. Three patients in the endoscopy group and 16 controls died. In the endoscopy group the correct preoperative diagnosis was made in all cases and there was less delay before operation. In the control group five patients had no diagnosis before operation, the preoperative diagnosis was wrong in nine, and five had laparotomies during which no cause of bleeding was found. The patients in the endoscopy group who did not have operations had a shorter stay in hospital than the controls.", "contents": "Comparative study between endoscopy and radiology in acute upper gastrointestinal haemorrhage. A total of 158 patients with acute upper gastrointestinal haemorrhage were studied, and the 53 patients on whom emergency endoscopies were performed were compared with the remaining 105. The cause of the bleeding was found in 51 of the endoscopy group and 39 of the control group. Three patients in the endoscopy group and 16 controls died. In the endoscopy group the correct preoperative diagnosis was made in all cases and there was less delay before operation. In the control group five patients had no diagnosis before operation, the preoperative diagnosis was wrong in nine, and five had laparotomies during which no cause of bleeding was found. The patients in the endoscopy group who did not have operations had a shorter stay in hospital than the controls."}
{"id": "PMID:1078986", "title": "Glutamic acid, GABA and their metabolising enzymes in the frog central nervous system.", "content": "The regional distribution of 9 amino acids, including glutamate and GABA and their metabolising enzymes, has been determined in 5 regions of the frog CNS. Glycine was relatively concentrated in the spinal cord whereas the highest concentration of each of the other amino acids was found in the midbrain. There was a good correlation between the activity of l-glutamate-1-carboxylase (GAD) and the level of GABA in all regions examined and both were concentrated in the midbrain. There was little regional variation in the distribution of 4-aminobutyrate-2-oxoglutarate transaminase (GABA-T).", "contents": "Glutamic acid, GABA and their metabolising enzymes in the frog central nervous system. The regional distribution of 9 amino acids, including glutamate and GABA and their metabolising enzymes, has been determined in 5 regions of the frog CNS. Glycine was relatively concentrated in the spinal cord whereas the highest concentration of each of the other amino acids was found in the midbrain. There was a good correlation between the activity of l-glutamate-1-carboxylase (GAD) and the level of GABA in all regions examined and both were concentrated in the midbrain. There was little regional variation in the distribution of 4-aminobutyrate-2-oxoglutarate transaminase (GABA-T)."}
{"id": "PMID:1078989", "title": "Penetration and survival of mesocercariae (Alaria spp.) in the mammalian eye.", "content": "A Canadian woman presented with a motile, intra-retinal worm, which by appearance and movement was strikingly similar to the mesocercaria of Alaria sp., a trematode from frogs. Using these mesocercariae and rabbits this study set out to establish 1) whether these mesocercariae can penetrate the cornea, 2) how long they might survive within the eye, and 3) what reaction is produced. It was found that some penetrated the cornea readily and, after injection, were capable of prolonged survival within the eye. Little overt reaction was produced though histologically the expected chronic inflammatory reaction with eosinophils occurred.", "contents": "Penetration and survival of mesocercariae (Alaria spp.) in the mammalian eye. A Canadian woman presented with a motile, intra-retinal worm, which by appearance and movement was strikingly similar to the mesocercaria of Alaria sp., a trematode from frogs. Using these mesocercariae and rabbits this study set out to establish 1) whether these mesocercariae can penetrate the cornea, 2) how long they might survive within the eye, and 3) what reaction is produced. It was found that some penetrated the cornea readily and, after injection, were capable of prolonged survival within the eye. Little overt reaction was produced though histologically the expected chronic inflammatory reaction with eosinophils occurred."}
{"id": "PMID:1078991", "title": "Analysis and reversal of the inhibition of cytophilic antibody receptors produced by antibody.", "content": "Administration of hyperimmune antibody to leukemia L1210 to allogeneic mice inhibited the development of macrophage-mediated immunity to L1210 in those hosts. In contrast to immunized mice, animals pretreated with antibody showed rapid activation of their peritoneal macrophages, followed by their disappearance and the inability of the residual peritoneal monocytic cells to attach L1210 cells even in the presence of proved cytophilic antibody to L1210. The inhibitory activity of the antibody, which resided entirely in its IgG2 fraction, was manifested only when the specific antigen (L1210 cells) was also injected within 2 days. Pretreatment with antibody to a different leukemia, EL4, failed to inhibit the monocytic uptake of L1210, but it did inhibit uptake of EL4 by monocytes if injected with its homologous antigen. Restoration of the functional capacity of macrophages was accomplished by injecting 1 X 10-7 bone marrow cells i.v. into \"suppressed\" mice, but 1.5 X 10-7 thymocytes failed to correct the defect. Significantly, thymocytes antagonized the restorative capability of bone marrow cells when they were injected concomitantly. These results indicate that specific inhibition of cytophilic antibody receptors on monocytes could be accomplished through a direct mechanism involving activation and exhaustion of macrophages and an indirect mechanism, perhaps mediated through \"suppressor\" thymus-derived cells. Although enhancement of the growth of leukemia cells did not occur, several parallels exist in mice with enhanced growth of different tumors. This inhibiotry phenomenon may thus represent another instance of \"blocking\" in tumor immunity, where the target of suppressive antibody-antigen is the macrophage as well as the lymphocyte.", "contents": "Analysis and reversal of the inhibition of cytophilic antibody receptors produced by antibody. Administration of hyperimmune antibody to leukemia L1210 to allogeneic mice inhibited the development of macrophage-mediated immunity to L1210 in those hosts. In contrast to immunized mice, animals pretreated with antibody showed rapid activation of their peritoneal macrophages, followed by their disappearance and the inability of the residual peritoneal monocytic cells to attach L1210 cells even in the presence of proved cytophilic antibody to L1210. The inhibitory activity of the antibody, which resided entirely in its IgG2 fraction, was manifested only when the specific antigen (L1210 cells) was also injected within 2 days. Pretreatment with antibody to a different leukemia, EL4, failed to inhibit the monocytic uptake of L1210, but it did inhibit uptake of EL4 by monocytes if injected with its homologous antigen. Restoration of the functional capacity of macrophages was accomplished by injecting 1 X 10-7 bone marrow cells i.v. into \"suppressed\" mice, but 1.5 X 10-7 thymocytes failed to correct the defect. Significantly, thymocytes antagonized the restorative capability of bone marrow cells when they were injected concomitantly. These results indicate that specific inhibition of cytophilic antibody receptors on monocytes could be accomplished through a direct mechanism involving activation and exhaustion of macrophages and an indirect mechanism, perhaps mediated through \"suppressor\" thymus-derived cells. Although enhancement of the growth of leukemia cells did not occur, several parallels exist in mice with enhanced growth of different tumors. This inhibiotry phenomenon may thus represent another instance of \"blocking\" in tumor immunity, where the target of suppressive antibody-antigen is the macrophage as well as the lymphocyte."}
{"id": "PMID:1078992", "title": "Ventricular performance and energy of compression, power, and rate of change of power during isovolumic contraction.", "content": "During isovolumic contraction, there is a calculabe compression of the blood within the ventricle. Energy is expended by the ventricle during isovolumic contraction, and some of it is transferred to the blood in the form of elastic compression. The rate of energy transfer (power) and acceleration of energy transfer (rate of change of power) during isovolumic contraction were calculated based upon considerations of the energy of compression. In anaesthetized dogs, the isovolumic energy of compression was 42 plus or minus 6 (mean plus or minus SE) dyn cm; peak isovolumic power was 1,400 plus or minus 300 dyn cm sec-1; and peak rate of change of power was 56,000 plus or minus 15,000 dyn cm sec-2. During states of augmented contractility induced by isoproterenol, the peak acceleration of energy expeniditure (peak rate of change of power) increased to 126,000 plus or minus 33,000 dyn cm sec-2 (p smaller than 0.001). Conversely, with a reduction of contractility induced by propranolol, the peak isovolumic rate of change of power decreased to 30,000 plus or minus 5,700 dyn cm sec-2 (p smaller than 0.001). The peak rate of change of power was unaffected by changes of the afterload. A trend, however, suggests that it may be affected by preload. The derivations of the isovolumic energy of compression, power, and rate of change of power are based upon firm principles of fluid dynamics. No assumptions related to ventricular geometry, synergy of contraction, or characteristics of muscle fibers are implied. Because of its physiological meaning and the theoretical validity of its derivation, an expression such as the isovolumic peak rate of change of power, when utilized as an index of ventricular performance, would appear to be of value.", "contents": "Ventricular performance and energy of compression, power, and rate of change of power during isovolumic contraction. During isovolumic contraction, there is a calculabe compression of the blood within the ventricle. Energy is expended by the ventricle during isovolumic contraction, and some of it is transferred to the blood in the form of elastic compression. The rate of energy transfer (power) and acceleration of energy transfer (rate of change of power) during isovolumic contraction were calculated based upon considerations of the energy of compression. In anaesthetized dogs, the isovolumic energy of compression was 42 plus or minus 6 (mean plus or minus SE) dyn cm; peak isovolumic power was 1,400 plus or minus 300 dyn cm sec-1; and peak rate of change of power was 56,000 plus or minus 15,000 dyn cm sec-2. During states of augmented contractility induced by isoproterenol, the peak acceleration of energy expeniditure (peak rate of change of power) increased to 126,000 plus or minus 33,000 dyn cm sec-2 (p smaller than 0.001). Conversely, with a reduction of contractility induced by propranolol, the peak isovolumic rate of change of power decreased to 30,000 plus or minus 5,700 dyn cm sec-2 (p smaller than 0.001). The peak rate of change of power was unaffected by changes of the afterload. A trend, however, suggests that it may be affected by preload. The derivations of the isovolumic energy of compression, power, and rate of change of power are based upon firm principles of fluid dynamics. No assumptions related to ventricular geometry, synergy of contraction, or characteristics of muscle fibers are implied. Because of its physiological meaning and the theoretical validity of its derivation, an expression such as the isovolumic peak rate of change of power, when utilized as an index of ventricular performance, would appear to be of value."}
{"id": "PMID:1078993", "title": "Influence of ambient temperature on growth rate of Reissner's fibre in Rana esculenta.", "content": "The influences of different environmental temperatures on the growth rate of Reissner's fibre (RF) in Rana esculenta were studied in October. At 24 degrees C about 70% and at 18 degrees C about 45% of the length of RF was renewed in 24 hours. This means that the growth rate of RF at 24 degrees C was about 50% faster than that at 18 degrees C. It strongly indicates that increase in ambient temperature from 18 degrees C to 24 degrees C stimulates the secretory activity of the SCO considerably at least as far as the production of RF is concerned. These results are discussed in view of the hypothesis that RF contributes to the regulation of the composition of the cerebrospinal fluid. In this connection some introductory observations on the capacity of RF to bind certain biogenic amines from the cerebrospinal fluid in Rana esculenta are presented.", "contents": "Influence of ambient temperature on growth rate of Reissner's fibre in Rana esculenta. The influences of different environmental temperatures on the growth rate of Reissner's fibre (RF) in Rana esculenta were studied in October. At 24 degrees C about 70% and at 18 degrees C about 45% of the length of RF was renewed in 24 hours. This means that the growth rate of RF at 24 degrees C was about 50% faster than that at 18 degrees C. It strongly indicates that increase in ambient temperature from 18 degrees C to 24 degrees C stimulates the secretory activity of the SCO considerably at least as far as the production of RF is concerned. These results are discussed in view of the hypothesis that RF contributes to the regulation of the composition of the cerebrospinal fluid. In this connection some introductory observations on the capacity of RF to bind certain biogenic amines from the cerebrospinal fluid in Rana esculenta are presented."}
{"id": "PMID:1078994", "title": "White pulp compartments in the spleen of rats and mice. A light and electron microscopic study of lymphoid and non-lymphoid celltypes in T- and B-areas.", "content": "The spleen of rats and mice was studied with the light and electron microscope. Special attention was paid to the delineation and composition of the white pulp compartments: periarteriolar lymphatic sheath (PALS), follicles and marginal zone. These three compartments each have their specific lymphoid and non-lymphoid cells. Reticulum cells and reticulin fibres, although occurring in all three compartments, from a characteristic pattern in each compartment. In the PALS two areas can be distinguished: a central area, largely devoid of reticulum cells, and a peripheral area where reticulum cells are arranged in cylindrical shells. The central PALS FORMS THE THYMUS DEPENDENT AREA OF THE SPLEEN, THE PERIPHERAL PALS contains both T- and B-lymphocytes. T-B-interactions requiring cell contact could take place in the latter area. Lymph vessels originate from the shells of reticulum cells around the smaller arterioles; these vessels follow the central arteriole to the hilus of the spleen. Circumstantial evidence suggests that the lymph vessels form a recirculation pathway for T-cells and possibly also for B-cells. In two areas of the splenic white pulp characteristic non-lymphoid cells are present. The central PALS contains interdigitating cells (IDC), which show a close contact with surrounding T-lymphocytes. The light zone of the follicle centre exhibits dendritic cells (DC). B-cells are found between the ramifications of the DC. It is conceivable that these cells play a role in the homing of T-cells and B-cells respectively. In addition they might create a microenvironment supporting differentiation and proliferation of T- and B-cells. The marginal zone does not contain a characteristic non-lymphoid cell type. However, in this compartment B-cells are directly exposed to the circulating blood. It is suggested that this factor constitutes one of the essentials of the microenvironment in the marginal zone.", "contents": "White pulp compartments in the spleen of rats and mice. A light and electron microscopic study of lymphoid and non-lymphoid celltypes in T- and B-areas. The spleen of rats and mice was studied with the light and electron microscope. Special attention was paid to the delineation and composition of the white pulp compartments: periarteriolar lymphatic sheath (PALS), follicles and marginal zone. These three compartments each have their specific lymphoid and non-lymphoid cells. Reticulum cells and reticulin fibres, although occurring in all three compartments, from a characteristic pattern in each compartment. In the PALS two areas can be distinguished: a central area, largely devoid of reticulum cells, and a peripheral area where reticulum cells are arranged in cylindrical shells. The central PALS FORMS THE THYMUS DEPENDENT AREA OF THE SPLEEN, THE PERIPHERAL PALS contains both T- and B-lymphocytes. T-B-interactions requiring cell contact could take place in the latter area. Lymph vessels originate from the shells of reticulum cells around the smaller arterioles; these vessels follow the central arteriole to the hilus of the spleen. Circumstantial evidence suggests that the lymph vessels form a recirculation pathway for T-cells and possibly also for B-cells. In two areas of the splenic white pulp characteristic non-lymphoid cells are present. The central PALS contains interdigitating cells (IDC), which show a close contact with surrounding T-lymphocytes. The light zone of the follicle centre exhibits dendritic cells (DC). B-cells are found between the ramifications of the DC. It is conceivable that these cells play a role in the homing of T-cells and B-cells respectively. In addition they might create a microenvironment supporting differentiation and proliferation of T- and B-cells. The marginal zone does not contain a characteristic non-lymphoid cell type. However, in this compartment B-cells are directly exposed to the circulating blood. It is suggested that this factor constitutes one of the essentials of the microenvironment in the marginal zone."}
{"id": "PMID:1078995", "title": "Alpha-1-antitrypsin in duodenal fluid and gallbladder bile.", "content": "Using the immunoelectrophoretic method alpha-1-antitrypsin was detected in all but 6 of 26 samples of duodenal fluid obtained from 16 patients with various gastroenterological problems. The concentrations (mg/100 ml) of alpha-1-antitrypsin in duodenal aspirates from children with liver disease (7.32 plus or minus 6.1) were less than those from children with cystic fibrosis, Shwachman Diamond syndrome, or Hirschsprung's disease (16.7 plus or minus 11.9; p smaller than 0.02). Alpha-1-antitrypsin was detected in all but one of 6 samples of gallbladder bile, the exception being that from a patient with extrahepatic biliary atresia. No significant correlation was found between the alpha-1-antitrypsin concentration in the samples studied and the corresponding total antitrypsin activity.", "contents": "Alpha-1-antitrypsin in duodenal fluid and gallbladder bile. Using the immunoelectrophoretic method alpha-1-antitrypsin was detected in all but 6 of 26 samples of duodenal fluid obtained from 16 patients with various gastroenterological problems. The concentrations (mg/100 ml) of alpha-1-antitrypsin in duodenal aspirates from children with liver disease (7.32 plus or minus 6.1) were less than those from children with cystic fibrosis, Shwachman Diamond syndrome, or Hirschsprung's disease (16.7 plus or minus 11.9; p smaller than 0.02). Alpha-1-antitrypsin was detected in all but one of 6 samples of gallbladder bile, the exception being that from a patient with extrahepatic biliary atresia. No significant correlation was found between the alpha-1-antitrypsin concentration in the samples studied and the corresponding total antitrypsin activity."}
{"id": "PMID:1078996", "title": "Tapeworm cyst infestations of the brain.", "content": "Tapeworm cyst infestations of the brain often present as diagnostic problems. The radiological appearances in proven cases are discussed with a brief review of the life cycle and clinical presentations.", "contents": "Tapeworm cyst infestations of the brain. Tapeworm cyst infestations of the brain often present as diagnostic problems. The radiological appearances in proven cases are discussed with a brief review of the life cycle and clinical presentations."}
{"id": "PMID:1079001", "title": "[Ulcerating duplication of the ileum as rare cause of massive intestinal hemorrhage in the adult].", "content": "In this paper is presented the case of a 27 year old woman in whom a congenital duplication of the distal ileum led to a massive hemorrhage. Heterotopic gastric mucosa was found in the ileal lesion and ulcer formation histologically proven. This case shows that congenital lesions of this sort may not come to the attention of the pediatrician or pediatric surgeon but become the concern of the surgeon in adult life.", "contents": "[Ulcerating duplication of the ileum as rare cause of massive intestinal hemorrhage in the adult]. In this paper is presented the case of a 27 year old woman in whom a congenital duplication of the distal ileum led to a massive hemorrhage. Heterotopic gastric mucosa was found in the ileal lesion and ulcer formation histologically proven. This case shows that congenital lesions of this sort may not come to the attention of the pediatrician or pediatric surgeon but become the concern of the surgeon in adult life."}
{"id": "PMID:1079005", "title": "Effects of the ultimobranchial and parathyroid glands and vitamins D2, D3 and dihydrotachysterol2 on blood calcium and intestinal calcium transport in the frog.", "content": "Administration of 500 mug vitamin D2 or D3 with ingestion of calcium to ultimobranchialectomized (UBX) or UBX-parathyroidectomized (PTX) frogs (Rana pipiens) induced hyerpcalcemia and hypercalciuria not apparent in control or PTX frogs. Calcium transport in isolated everted gut sacs was significantly elevated in UBX and UBX-PTX frogs but not in controls or PTX animals. Further, with Vitamin D3 in UBX frogs the duodenal segment had a greater capacity to transport calcium than the jejunal-ileal segment when compared to control, PTX or UBX-PTX frogs. Dihydrotachysterol2 and calcium ingestion also induced hypercalcemia, hypercalciuria and increased calcium transport in gut of UBX and UBX-PTX frogs, with no change seen in PTX or controls after 5 days. The inhibitory influence of the ultimobranchial glands on intestinal calcium transport apparently does not require the presence of the parathyroids and exhibits an inhibitory influence against a high calcium gradient across the duodenal segment.", "contents": "Effects of the ultimobranchial and parathyroid glands and vitamins D2, D3 and dihydrotachysterol2 on blood calcium and intestinal calcium transport in the frog. Administration of 500 mug vitamin D2 or D3 with ingestion of calcium to ultimobranchialectomized (UBX) or UBX-parathyroidectomized (PTX) frogs (Rana pipiens) induced hyerpcalcemia and hypercalciuria not apparent in control or PTX frogs. Calcium transport in isolated everted gut sacs was significantly elevated in UBX and UBX-PTX frogs but not in controls or PTX animals. Further, with Vitamin D3 in UBX frogs the duodenal segment had a greater capacity to transport calcium than the jejunal-ileal segment when compared to control, PTX or UBX-PTX frogs. Dihydrotachysterol2 and calcium ingestion also induced hypercalcemia, hypercalciuria and increased calcium transport in gut of UBX and UBX-PTX frogs, with no change seen in PTX or controls after 5 days. The inhibitory influence of the ultimobranchial glands on intestinal calcium transport apparently does not require the presence of the parathyroids and exhibits an inhibitory influence against a high calcium gradient across the duodenal segment."}
{"id": "PMID:1079007", "title": "Calcium transients in amphibian muscle.", "content": "The calcium-sensitive bioluminescent protein aequorin has been microinjected into isolated amphibian twitch muscle fibers in order to detect the intracellular calcium transients associated with excitation-contraction coupling. While it is not yet possible to make quantitative estimates of the changes in calcium concentration involved, it is possible to detect substantial changes in intracellular calcium transients resulting from changes in temperature, stimulation frequency, fiberlength, and the osmotic strength of the bathing medium.", "contents": "Calcium transients in amphibian muscle. The calcium-sensitive bioluminescent protein aequorin has been microinjected into isolated amphibian twitch muscle fibers in order to detect the intracellular calcium transients associated with excitation-contraction coupling. While it is not yet possible to make quantitative estimates of the changes in calcium concentration involved, it is possible to detect substantial changes in intracellular calcium transients resulting from changes in temperature, stimulation frequency, fiberlength, and the osmotic strength of the bathing medium."}
{"id": "PMID:1079008", "title": "Membrane particles and transmission at the triad.", "content": "The structure of the membranes limiting the sarcoplasmic reticulum (SR) and transverse (T) tubules in frog and fish muscle fibers has been studied by freeze fracture. Emphasis is placed on the structure of the membranes at the triad, where thin sections have previously shown that rows of regularly disposed \"feet\" join SR and T exposed fracture faces allows the following conclusions. 1) The SR membrane is continuous and identical in appearance along the whole sarcomere. Thus the SR is a single uninterrupted compartment and it is likely that the major function of the reticulum, calcium accumulation is performed by the membrane limiting the lateral sacs of the triad, as well as the longitudinal tubules. 2) At the level of the junction with the T tublue, the SR presents a strikingly different number, size and arrangement of particles and pits. This distinct portion of the SR membrane extends father than the area covered by the junctional \"feet\" and no correlation can be found between the disposition of particles within the membrane and that of the feet on the membrane surface. 3) The T system membrane presents few prominent particles on its junctional face, but these are far less numerous than the feet. 4) Thus, no visible preformed channels exist between SR and T system lumina and it is suggested that direct electrical coupling between the two membranes during excitation is unlikely.", "contents": "Membrane particles and transmission at the triad. The structure of the membranes limiting the sarcoplasmic reticulum (SR) and transverse (T) tubules in frog and fish muscle fibers has been studied by freeze fracture. Emphasis is placed on the structure of the membranes at the triad, where thin sections have previously shown that rows of regularly disposed \"feet\" join SR and T exposed fracture faces allows the following conclusions. 1) The SR membrane is continuous and identical in appearance along the whole sarcomere. Thus the SR is a single uninterrupted compartment and it is likely that the major function of the reticulum, calcium accumulation is performed by the membrane limiting the lateral sacs of the triad, as well as the longitudinal tubules. 2) At the level of the junction with the T tublue, the SR presents a strikingly different number, size and arrangement of particles and pits. This distinct portion of the SR membrane extends father than the area covered by the junctional \"feet\" and no correlation can be found between the disposition of particles within the membrane and that of the feet on the membrane surface. 3) The T system membrane presents few prominent particles on its junctional face, but these are far less numerous than the feet. 4) Thus, no visible preformed channels exist between SR and T system lumina and it is suggested that direct electrical coupling between the two membranes during excitation is unlikely."}
{"id": "PMID:1079009", "title": "In vitro responsiveness of lymphocytes of different mouse strains to phytohaemagglutinin.", "content": "Responsiveness of spleen and lymph node lymphocytes to in vitro stimulation by phytohaemagglutinin was investigated in several mouse strains. Considerable interstain differences in RNA and DNA synthesis were found both for PHA-stimulated and unstimulated cells; the latter component thus tends to contribute to the observed over-all differences in lymphocyte responsiveness to \"specific\" stimulation. Of the tested strains and F-1 HYBRIDS, THE HIGHEST RESPONDERS WERE A/Ph, its F-1 hybrids with a few other strains and BALB/C. The possibility that the interstrain differences in the rate of nucleic acid synthesis and hence in lymphocyte responsiveness could be due to different sensitivity of the lymphocytes to experimental treatment was not confirmed experimentally. Relatively high rate of synthesis of nucleic acids in PHA unstimulated cells and its great interstrain variation is interpreted in terms of variation in \"spontaneous\" transformability.", "contents": "In vitro responsiveness of lymphocytes of different mouse strains to phytohaemagglutinin. Responsiveness of spleen and lymph node lymphocytes to in vitro stimulation by phytohaemagglutinin was investigated in several mouse strains. Considerable interstain differences in RNA and DNA synthesis were found both for PHA-stimulated and unstimulated cells; the latter component thus tends to contribute to the observed over-all differences in lymphocyte responsiveness to \"specific\" stimulation. Of the tested strains and F-1 HYBRIDS, THE HIGHEST RESPONDERS WERE A/Ph, its F-1 hybrids with a few other strains and BALB/C. The possibility that the interstrain differences in the rate of nucleic acid synthesis and hence in lymphocyte responsiveness could be due to different sensitivity of the lymphocytes to experimental treatment was not confirmed experimentally. Relatively high rate of synthesis of nucleic acids in PHA unstimulated cells and its great interstrain variation is interpreted in terms of variation in \"spontaneous\" transformability."}
{"id": "PMID:1079010", "title": "Teratogenesis and the movement of ions.", "content": "There is evidence that in early embryos, mechanisms exist for the control of the movement of monovalent ions; that sodium is extruded during gastrulation and neurulation, and that in two- to three-day-old chick embryos with head abnormalities, ratios of sodium to potassium may be abnormally high. It is suggested that the origin of neural tube defects in early development may be associated with failure of control of ionic movements.", "contents": "Teratogenesis and the movement of ions. There is evidence that in early embryos, mechanisms exist for the control of the movement of monovalent ions; that sodium is extruded during gastrulation and neurulation, and that in two- to three-day-old chick embryos with head abnormalities, ratios of sodium to potassium may be abnormally high. It is suggested that the origin of neural tube defects in early development may be associated with failure of control of ionic movements."}
{"id": "PMID:1079015", "title": "Lymphoid cell dependence of eosinophil response to antigen. VI. The effect of selective removal of T or B lymphocytes on the capacity of primed spleen cells to adoptively transferred immunity to tetanus toxoid.", "content": "Spleens from mice primed with tetanus toxoid 30 days earlier contain memory cells capable of adoptively transferring secondary type cell-mediated (eosinophil) and humoral (antitoxin) responses to irradiated, reconstituted recipients. Spleen cells derived from 10-day-primed donors, on the other hand, possess the capacity after transfer to elicit secondary type eosinophil responses, but not anamnestic antitoxin responses. Treatment of 30-day-primed cells with anti-theta serum and C' prevented transfer of memory for both responses, whereas similar treatment with rabbit anti-mouse IgG (RAM-IgG) serum and C' only inhibited transfer of memory for the antitoxin response. Addition of non-primed spleen cells to antisera-treated primed cells failed to restore secondary type responses. Recombination of 30-day-primed anti-theta and RAM-IgG-treated cells re-established the capacity to transfer these responses. To determine whether the same T cells which mediate the eosiniphil response also act as helper cells in antitoxin production, antisera treated 10- and 30-day-primed cells were combined prior to transfer. Ten-day-primed T cells induced eisoniphil responses and also co-operated with 30-day-primed B cells to produce antitoxin. In contrast, 30-day-primed T cells elicited eisinophil responses, but were unable to induce antitoxin production when combined with anti-theta-treated 10-day-primed cells. These results indicate that B memory cells are not present in the spleens of the donor mice 10 days after priming, but T memory cells are present. It is concluded that primed T cells mediated both eosinophil and antitoxin responses, while B memory cells are involved only with antitoxin production. Following subcutaneous priming T memory cells are present in the spleen prior to B memory cells, and T memory cells which mediate the eosinophil response at 10 days after priming also augment the production of antitoxin by B memory cells.", "contents": "Lymphoid cell dependence of eosinophil response to antigen. VI. The effect of selective removal of T or B lymphocytes on the capacity of primed spleen cells to adoptively transferred immunity to tetanus toxoid. Spleens from mice primed with tetanus toxoid 30 days earlier contain memory cells capable of adoptively transferring secondary type cell-mediated (eosinophil) and humoral (antitoxin) responses to irradiated, reconstituted recipients. Spleen cells derived from 10-day-primed donors, on the other hand, possess the capacity after transfer to elicit secondary type eosinophil responses, but not anamnestic antitoxin responses. Treatment of 30-day-primed cells with anti-theta serum and C' prevented transfer of memory for both responses, whereas similar treatment with rabbit anti-mouse IgG (RAM-IgG) serum and C' only inhibited transfer of memory for the antitoxin response. Addition of non-primed spleen cells to antisera-treated primed cells failed to restore secondary type responses. Recombination of 30-day-primed anti-theta and RAM-IgG-treated cells re-established the capacity to transfer these responses. To determine whether the same T cells which mediate the eosiniphil response also act as helper cells in antitoxin production, antisera treated 10- and 30-day-primed cells were combined prior to transfer. Ten-day-primed T cells induced eisoniphil responses and also co-operated with 30-day-primed B cells to produce antitoxin. In contrast, 30-day-primed T cells elicited eisinophil responses, but were unable to induce antitoxin production when combined with anti-theta-treated 10-day-primed cells. These results indicate that B memory cells are not present in the spleens of the donor mice 10 days after priming, but T memory cells are present. It is concluded that primed T cells mediated both eosinophil and antitoxin responses, while B memory cells are involved only with antitoxin production. Following subcutaneous priming T memory cells are present in the spleen prior to B memory cells, and T memory cells which mediate the eosinophil response at 10 days after priming also augment the production of antitoxin by B memory cells."}
{"id": "PMID:1079016", "title": "The mechanism of interaction between T and B lymphocytes in the in vitro response to sheep erythrocytes. Non-specific collaboration across a dialysis membrane.", "content": "Purified T lymphocytes from normal mouse spleen restored the antibody-forming cell response to sheep erythrocytes of various B lymphocyte populations when separated from them by a cell-impermeable dialysis or nucleopore membrane. A 2-5-fold increase occurred when spleen cells from neonatally thymectomized mice or congenitally athymic mice, and an adherent spleen cell population were used as sources of B cells. Antigen was required in both T- and B-cell compartments, but a nonspecific reconstitution occurred when the antigens present in the two compartments were different. It is concluded that during the first 20 hours of culture, T lymphocytes produce a non-specific factor in response to antigen. Although the factor acts at a distance, it does not have a non-specific mitogenic effect upon all B lymphocytes. Some of its properties are similar to those of other reported T-cell factors and of the 'sheep erythrocyte reconstitution factor', found naturally in some batches of foetal calf serum.", "contents": "The mechanism of interaction between T and B lymphocytes in the in vitro response to sheep erythrocytes. Non-specific collaboration across a dialysis membrane. Purified T lymphocytes from normal mouse spleen restored the antibody-forming cell response to sheep erythrocytes of various B lymphocyte populations when separated from them by a cell-impermeable dialysis or nucleopore membrane. A 2-5-fold increase occurred when spleen cells from neonatally thymectomized mice or congenitally athymic mice, and an adherent spleen cell population were used as sources of B cells. Antigen was required in both T- and B-cell compartments, but a nonspecific reconstitution occurred when the antigens present in the two compartments were different. It is concluded that during the first 20 hours of culture, T lymphocytes produce a non-specific factor in response to antigen. Although the factor acts at a distance, it does not have a non-specific mitogenic effect upon all B lymphocytes. Some of its properties are similar to those of other reported T-cell factors and of the 'sheep erythrocyte reconstitution factor', found naturally in some batches of foetal calf serum."}
{"id": "PMID:1079017", "title": "Growth of Mycobacterium marinum in the footpads of T-cell-depleted mice.", "content": "Mycobacterium marinum strains 1218 and 1219 were inoculated into the hind footpads of T-cell-depleted specific pathogen-free C57B1/6 mice, and the growth and survival of the organisms at the site of injection, the draining popliteal lymph node, and the spleen and lung were quantitated for up to 70 days. T-cell depletion largely ablated the normal cell-mediated antituberculous response to the M. marinum population. The mice were able to control the further growth of the inoculum within the footpad only after it had reached 5 to 10 times that present in the normal controls. The high temperature-adapted strain (37 C; strain no. 1218) induced an increasing infection in the liver, spleen, and lungs of the THXB mice, and the infection eventually spread to the opposite footpad and to the tail skin. Strain 1219 gave rise to considerable systemic involvement in the THXB host despite its inability to survive at 37 C, but the size of the splenic and lung populations was considerably lower than in the 1218-infected animals. Both M. marinum infections persisted in the tissues of the T-cell-depleted mice with no indication of a cell-mediated immune response. Footpad swelling in the M. marinum-infected mice was not greatly reduced by T-cell depletion, and, if anything, tended to persist at high levels long after the swelling of the control feet had gone into a decline. On the other hand, incorporation of tritiated thymidine by cells within the infected footpads, the draining lymph node, and the spleen was considerably reduced in the T-cell-depleted host compared with control values. Late in the infection, there was a significant increase in the amount of label taken up by the cells in the footpads of the T-cell-depleted host.", "contents": "Growth of Mycobacterium marinum in the footpads of T-cell-depleted mice. Mycobacterium marinum strains 1218 and 1219 were inoculated into the hind footpads of T-cell-depleted specific pathogen-free C57B1/6 mice, and the growth and survival of the organisms at the site of injection, the draining popliteal lymph node, and the spleen and lung were quantitated for up to 70 days. T-cell depletion largely ablated the normal cell-mediated antituberculous response to the M. marinum population. The mice were able to control the further growth of the inoculum within the footpad only after it had reached 5 to 10 times that present in the normal controls. The high temperature-adapted strain (37 C; strain no. 1218) induced an increasing infection in the liver, spleen, and lungs of the THXB mice, and the infection eventually spread to the opposite footpad and to the tail skin. Strain 1219 gave rise to considerable systemic involvement in the THXB host despite its inability to survive at 37 C, but the size of the splenic and lung populations was considerably lower than in the 1218-infected animals. Both M. marinum infections persisted in the tissues of the T-cell-depleted mice with no indication of a cell-mediated immune response. Footpad swelling in the M. marinum-infected mice was not greatly reduced by T-cell depletion, and, if anything, tended to persist at high levels long after the swelling of the control feet had gone into a decline. On the other hand, incorporation of tritiated thymidine by cells within the infected footpads, the draining lymph node, and the spleen was considerably reduced in the T-cell-depleted host compared with control values. Late in the infection, there was a significant increase in the amount of label taken up by the cells in the footpads of the T-cell-depleted host."}
{"id": "PMID:1079018", "title": "Survival of synchronized Chinese hamster cells exposed to radiation of different linear-energy transfer.", "content": "Chinese hamster V79 cells were exposed to ionizing radiations of a wide range of linear-energy transfer (LET), including 145kV x-rays and six different heavy ions accelerated in the Berkeley heavy-ion linear accelerator. The LET of the ions ranged from 19 keV/um to 2000 keV/um. Survival curves were determined for both synchronized and asynchronous cells, using survival of colony-forming capacity as the end-point. Results with asynchronous cultures were similar to results reported previously for mammalian cells. There was increased effectiveness of killing per dose with increased LET until a change in shape of the single-cell survival curves resulted, from sigmoidal to exponential, with carbon ions (LET of 190 keV/um). With heavier ions, exponential curves were obtained, but with decreased effectiveness per unit dose. Synchronized cultures were obtained by mitotic selection. The expected Chinese hamster cell-cycle survival curve variation was found for X-rays, mainly reflecting the variation in the single-cell extrapolation number, with late S-phase cells the most resistant to radiation. When synchronized cultures were irradiated with the heavy ions that produce exponential survival curves; the survival curves were independent of the cell-cycle time of irradiation. With radiations of LET values between the low and high extremes, a reduced cell-cycle survival curve variation was found, indicating a gradual reduction in the cell-cycle survival curve variation as a function of increased LET.", "contents": "Survival of synchronized Chinese hamster cells exposed to radiation of different linear-energy transfer. Chinese hamster V79 cells were exposed to ionizing radiations of a wide range of linear-energy transfer (LET), including 145kV x-rays and six different heavy ions accelerated in the Berkeley heavy-ion linear accelerator. The LET of the ions ranged from 19 keV/um to 2000 keV/um. Survival curves were determined for both synchronized and asynchronous cells, using survival of colony-forming capacity as the end-point. Results with asynchronous cultures were similar to results reported previously for mammalian cells. There was increased effectiveness of killing per dose with increased LET until a change in shape of the single-cell survival curves resulted, from sigmoidal to exponential, with carbon ions (LET of 190 keV/um). With heavier ions, exponential curves were obtained, but with decreased effectiveness per unit dose. Synchronized cultures were obtained by mitotic selection. The expected Chinese hamster cell-cycle survival curve variation was found for X-rays, mainly reflecting the variation in the single-cell extrapolation number, with late S-phase cells the most resistant to radiation. When synchronized cultures were irradiated with the heavy ions that produce exponential survival curves; the survival curves were independent of the cell-cycle time of irradiation. With radiations of LET values between the low and high extremes, a reduced cell-cycle survival curve variation was found, indicating a gradual reduction in the cell-cycle survival curve variation as a function of increased LET."}
{"id": "PMID:1079019", "title": "Chromosomal aberrations in rats fed irradiated wheat.", "content": "The effects on chromosomal aberrations in bone-marrow cells of feeding irradiated wheat to well-fed and malnourished rats were investigated. Rats maintained on low levels of dietary protein (5 per cent) had a higher incidence of breaks and deletions in their bone-marrow chromosomes compared with rats maintained on  adequate levels of protein  (18 per cient), showing that the level of dietary protein per se can produce structural abnormalities. Feeding of irradiated wheat to rats was associated with increase in the number of polyploid cells in the bone-marrow. The level of protein in the diet did not appear to influence polyploidy.", "contents": "Chromosomal aberrations in rats fed irradiated wheat. The effects on chromosomal aberrations in bone-marrow cells of feeding irradiated wheat to well-fed and malnourished rats were investigated. Rats maintained on low levels of dietary protein (5 per cent) had a higher incidence of breaks and deletions in their bone-marrow chromosomes compared with rats maintained on  adequate levels of protein  (18 per cient), showing that the level of dietary protein per se can produce structural abnormalities. Feeding of irradiated wheat to rats was associated with increase in the number of polyploid cells in the bone-marrow. The level of protein in the diet did not appear to influence polyploidy."}
{"id": "PMID:1079020", "title": "[Role of DNA-membrane attachment in repair of radiation damage in Micrococcus radiodurans].", "content": "The unirradiated bacterial DNA assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from X-ray action. During the reincubation period in growth-medium, the DNA is reassociated with the membrane. This phenomenon is very rapid and occurs without increasing the molecular weight of DNA. The study of DNA-membrane complexes shows that the size of the DNA-associated membranous fragment differs according to the lysing technique employed, appearing as a change in the density of the complex. Chloramphenicol decreases reassociation, and iodoacetamide, a radiosensititzing agent, inhibits it completely.", "contents": "[Role of DNA-membrane attachment in repair of radiation damage in Micrococcus radiodurans]. The unirradiated bacterial DNA assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from X-ray action. During the reincubation period in growth-medium, the DNA is reassociated with the membrane. This phenomenon is very rapid and occurs without increasing the molecular weight of DNA. The study of DNA-membrane complexes shows that the size of the DNA-associated membranous fragment differs according to the lysing technique employed, appearing as a change in the density of the complex. Chloramphenicol decreases reassociation, and iodoacetamide, a radiosensititzing agent, inhibits it completely."}
{"id": "PMID:1079021", "title": "Effect of ethanol on the radiolysis of ribonuclease.", "content": "The dependence of D 37 on the ribonuclease concentration was measured for depolymerase and hydrolase activity. Ethanol gives different radiation protection to the two functions by OH-scavenging. Evidence for the reaction of ethanol radicals was obtained by simultaneously scavening hydrated electrons, OH-and most of the H-radicals without increase in the radiation protection. The efficiency of the individual primary radicals and the ethanol radicals in inactiviating ribonuclease is calculated from the measured inactivation yield.", "contents": "Effect of ethanol on the radiolysis of ribonuclease. The dependence of D 37 on the ribonuclease concentration was measured for depolymerase and hydrolase activity. Ethanol gives different radiation protection to the two functions by OH-scavenging. Evidence for the reaction of ethanol radicals was obtained by simultaneously scavening hydrated electrons, OH-and most of the H-radicals without increase in the radiation protection. The efficiency of the individual primary radicals and the ethanol radicals in inactiviating ribonuclease is calculated from the measured inactivation yield."}
{"id": "PMID:1079022", "title": "Mechanisms of radio-protection by catecholamines in the hamster (Mesocricetus auratus).", "content": "Whole-body exposure to 1000 or 2000 rads in a 60Co source was used to study radio-protection by norepinephrine, isoproterenol, and phenylephrine in normal and splenectomized hamsters. After exposure, the animals were caged individually,  and percent survivals were recorded daily. The aortic blood pressure of permanently cannulated, unanaesthetized hamsters was measured to test the vasoconstriction-hypoxia mechanism of radio-protection, and plasma calcium levels were measured to test the hypothesis that isoproterenol protects through a mechanism of hypercalcaemia-stimualted cell proliferation. The data support the vasoconstriction-hypoxia mechanism for agents stimulating the alpha receptors, while beta agonists seem to protect through a hypotensive-hypoxia mechanism similar to that of histamine. All the catecholamines protect against the haematopoietic syndrome, but show no evidence of protection against the gastrointestinal syndrome.", "contents": "Mechanisms of radio-protection by catecholamines in the hamster (Mesocricetus auratus). Whole-body exposure to 1000 or 2000 rads in a 60Co source was used to study radio-protection by norepinephrine, isoproterenol, and phenylephrine in normal and splenectomized hamsters. After exposure, the animals were caged individually,  and percent survivals were recorded daily. The aortic blood pressure of permanently cannulated, unanaesthetized hamsters was measured to test the vasoconstriction-hypoxia mechanism of radio-protection, and plasma calcium levels were measured to test the hypothesis that isoproterenol protects through a mechanism of hypercalcaemia-stimualted cell proliferation. The data support the vasoconstriction-hypoxia mechanism for agents stimulating the alpha receptors, while beta agonists seem to protect through a hypotensive-hypoxia mechanism similar to that of histamine. All the catecholamines protect against the haematopoietic syndrome, but show no evidence of protection against the gastrointestinal syndrome."}
{"id": "PMID:1079025", "title": "Multiple cartilaginous exostoses in the dog.", "content": "The clinicopathologic aspects of multiple cartilaginous exostoses (MCE) in 2 dogs were compared with those in 9 previously reported cases of MCE in dogs. Although a familial tendency is probable, there is apparently no sex or breed predilection. Only bones that developed by endochondral ossification were affected. The vertebrae, ribs, and long bones were the most frequent location of exostoses. Bones of the carpal and tarsal joints and the skull were not affected in the cases evaluated. Physical examination and radiography may provide strong supportive evidence for a diagnosis of MCE, but a definitive diagnosis must be based on microscopic evaluation of osseous lesions. Treatment is unnecessary unless growth of exostoses results in clinical sequelae. Surgical extirpation of lesions should be considered if dysfunction of the skeletal, muscular, or neurologic systems develops. The prognosis is variable, being dependent on the location and number of lesions, the age of the patient at the time of diagnosis, and the presence or absence of clinical complications.", "contents": "Multiple cartilaginous exostoses in the dog. The clinicopathologic aspects of multiple cartilaginous exostoses (MCE) in 2 dogs were compared with those in 9 previously reported cases of MCE in dogs. Although a familial tendency is probable, there is apparently no sex or breed predilection. Only bones that developed by endochondral ossification were affected. The vertebrae, ribs, and long bones were the most frequent location of exostoses. Bones of the carpal and tarsal joints and the skull were not affected in the cases evaluated. Physical examination and radiography may provide strong supportive evidence for a diagnosis of MCE, but a definitive diagnosis must be based on microscopic evaluation of osseous lesions. Treatment is unnecessary unless growth of exostoses results in clinical sequelae. Surgical extirpation of lesions should be considered if dysfunction of the skeletal, muscular, or neurologic systems develops. The prognosis is variable, being dependent on the location and number of lesions, the age of the patient at the time of diagnosis, and the presence or absence of clinical complications."}
{"id": "PMID:1079026", "title": "Studies on the mechanism of action of calciferol. VIII. The effects of dietary vitamin D and the polyene antibiotic, filipin, in vitro, on the intestinal cellular uptake of calcium.", "content": "Intestinal transport and cellular uptake of calcium were studied in vitro in ileal segments obtained from calciferol (vitamin D)-deficient (minus D3) chicks or in calciferol-treated chicks (+ D3). When calcium flux (J) was measured in both the mucosal yields serosal (J-ms) and serosal yields mucosal (J-sm) directions it was found that calcium transport in the +D3 system was an active process. Calcium was not actively transported in the minus D3 system or by either the +D3 or minus D3 serosal tissues which underlie the intestinal mucosa tissue. It was also found that the serosal tissue was not the rate-limiting step in calcium translocation. When calcium uptake was measured at the mucosal or brush border surface it was found to be a cation-oriented, saturable process in both the +D3 and minus D3 systems and was enhanced by calciferol supplementation. The rate of uptake was found to exhibit a two component transport phenomenon, one a saturable process at low Ca2+ concentrations and the other a linear of diffusion process at higher Ca2+ concentrations (greater than 5 mM). The polyene antibiotic, filipin, was used to study the transport and uptake of calcium in both the +D and minusD chick ileum in vitro. Filipin (10 mug/ml), when added in vitro to solutions bathing the mucosal surface of the ileum, stimulated the calcium flux, J-ms, of minus D ileal tissue by 150 to 200%, but had little or no stimulatory effect on J-ms of +D3 ileal tissue. However, in contrast it stimulated calcium uptake across the mucosal membrane in both the +D3 and minus D3 ileal tissue (50 to 100%). The effect of filipin is specific for calcium uptake as compared to Rb+, P-i, SO4(2-), glycerol, thiourea, and urea uptake. Isolated brush borders from +D3 chicks were found to bind calcium to a greater extent than the minus D3 brush borders, but both membrane fractions had the same cholesterol content. The sum of this and other evidence suggests that of uptake of calcium is a calciferol-mediated event which is not the rate-limiting step in the total transport of this ion across the intestinal epithelial cell. The filipin effect appears to affect a structural reorganization of the brush border membrane in both the +D3 and minus D3 membranes in a manner specific for calcium translocation. It now appears that filipin treatment somehow makes both transport systems more efficient in the uptake of calcium, but in a manner independent of the mode of action of calciferol. However, these data suggest that other components associated with calcium uptake are calciferol-dependent in an as yet unknown fashion.", "contents": "Studies on the mechanism of action of calciferol. VIII. The effects of dietary vitamin D and the polyene antibiotic, filipin, in vitro, on the intestinal cellular uptake of calcium. Intestinal transport and cellular uptake of calcium were studied in vitro in ileal segments obtained from calciferol (vitamin D)-deficient (minus D3) chicks or in calciferol-treated chicks (+ D3). When calcium flux (J) was measured in both the mucosal yields serosal (J-ms) and serosal yields mucosal (J-sm) directions it was found that calcium transport in the +D3 system was an active process. Calcium was not actively transported in the minus D3 system or by either the +D3 or minus D3 serosal tissues which underlie the intestinal mucosa tissue. It was also found that the serosal tissue was not the rate-limiting step in calcium translocation. When calcium uptake was measured at the mucosal or brush border surface it was found to be a cation-oriented, saturable process in both the +D3 and minus D3 systems and was enhanced by calciferol supplementation. The rate of uptake was found to exhibit a two component transport phenomenon, one a saturable process at low Ca2+ concentrations and the other a linear of diffusion process at higher Ca2+ concentrations (greater than 5 mM). The polyene antibiotic, filipin, was used to study the transport and uptake of calcium in both the +D and minusD chick ileum in vitro. Filipin (10 mug/ml), when added in vitro to solutions bathing the mucosal surface of the ileum, stimulated the calcium flux, J-ms, of minus D ileal tissue by 150 to 200%, but had little or no stimulatory effect on J-ms of +D3 ileal tissue. However, in contrast it stimulated calcium uptake across the mucosal membrane in both the +D3 and minus D3 ileal tissue (50 to 100%). The effect of filipin is specific for calcium uptake as compared to Rb+, P-i, SO4(2-), glycerol, thiourea, and urea uptake. Isolated brush borders from +D3 chicks were found to bind calcium to a greater extent than the minus D3 brush borders, but both membrane fractions had the same cholesterol content. The sum of this and other evidence suggests that of uptake of calcium is a calciferol-mediated event which is not the rate-limiting step in the total transport of this ion across the intestinal epithelial cell. The filipin effect appears to affect a structural reorganization of the brush border membrane in both the +D3 and minus D3 membranes in a manner specific for calcium translocation. It now appears that filipin treatment somehow makes both transport systems more efficient in the uptake of calcium, but in a manner independent of the mode of action of calciferol. However, these data suggest that other components associated with calcium uptake are calciferol-dependent in an as yet unknown fashion."}
{"id": "PMID:1079027", "title": "Complications of Austin Moore arthroplasty. Their incidence and relationship to potential predisposing factors.", "content": "In a retrospective computerized study of 451 Austin Moore arthroplasties (211 for acute hip fractures and 240 for hip reconstruction), the operative and general complications were correlated with the historical, preoperative, operative, and early and late postoperative factors usually thought to influence the results. Wound complications were significantly associated with diabetes and fracture, obesity and fracture, and procedures lasting two hours or more. General complications showing significant associations were: death or pneumonia with fractures, phlebitis with obesity, and myocardial infarction with fracture. Factors not significantly associated with complications included age, previous hip surgery, time of day of the operation, surgical approach, wound irrigation, use of drains, postoperative anticoagulation, and reaming of the acetabulum.", "contents": "Complications of Austin Moore arthroplasty. Their incidence and relationship to potential predisposing factors. In a retrospective computerized study of 451 Austin Moore arthroplasties (211 for acute hip fractures and 240 for hip reconstruction), the operative and general complications were correlated with the historical, preoperative, operative, and early and late postoperative factors usually thought to influence the results. Wound complications were significantly associated with diabetes and fracture, obesity and fracture, and procedures lasting two hours or more. General complications showing significant associations were: death or pneumonia with fractures, phlebitis with obesity, and myocardial infarction with fracture. Factors not significantly associated with complications included age, previous hip surgery, time of day of the operation, surgical approach, wound irrigation, use of drains, postoperative anticoagulation, and reaming of the acetabulum."}
{"id": "PMID:1079028", "title": "Direct-current stimulation of non-union and congenital pseudarthrosis. Exploration of its clinical application.", "content": "Based on the response of bone to electrical current in previous studies, an exploratory clinical study of the effect of electrical current on non-union and congenital pseudarthrosis was performed. Constant direct current of ten to twenty microamperes was applied to twenty-four non-unions and five congenital pseudarthroses. Complete union occurred in fifteen non-unions and in one congenital pseudarthrosis. A single cathode delivering ten microamperes seemed sufficient to heal non-union in small bones, but multiple cathodes each delivering twenty microamperes appeared to be required to heal non-union in the tibia and femur. As the technique was refined, the percentage of successes increased. While these preliminary results appear promising, further laboratory and clinical experiments are required to define the true role of electrical stimulation in clinical practice.", "contents": "Direct-current stimulation of non-union and congenital pseudarthrosis. Exploration of its clinical application. Based on the response of bone to electrical current in previous studies, an exploratory clinical study of the effect of electrical current on non-union and congenital pseudarthrosis was performed. Constant direct current of ten to twenty microamperes was applied to twenty-four non-unions and five congenital pseudarthroses. Complete union occurred in fifteen non-unions and in one congenital pseudarthrosis. A single cathode delivering ten microamperes seemed sufficient to heal non-union in small bones, but multiple cathodes each delivering twenty microamperes appeared to be required to heal non-union in the tibia and femur. As the technique was refined, the percentage of successes increased. While these preliminary results appear promising, further laboratory and clinical experiments are required to define the true role of electrical stimulation in clinical practice."}
{"id": "PMID:1079029", "title": "Extractable nuclear antigen effect on the DNA anti-DNA reaction and NZB/NZW mouse nephritis.", "content": "Extractable nuclear antigen (ENA) is composed of at least two components, one a ribonucleo-protein sensitive to ribonuclease or heat and the other a protein. Antibodies to ENA are associated with a relatively benign clinical course in patients with systemic lupus erythematosus (SLE) in which DNA anti DNA complexes are thought pathogenic. The effect of ENA and anti-ENA on DNA anti-DNA reactions in vitro was studied. ENA effectively inhibited an anti-DNA hemagglutination reaction but no effect was found on binding of radioactive DNA or on the anti-hemocyanin hemagglutination reaction. The inhibitory effect was not abolished by yeast ribonuclease (RNase), heating, or DNase. Anti-ENA HAD NO EFFECT ON ANTI-DNA hemagglutination. In vivo, ENA altered the NZB/NZW mouse nephritis thought to be a model for human SLE nephritis. These results suggest the possiblity of a role for ENA in alteration of diseases due to pathogenic DNA anti-DNA complexes.", "contents": "Extractable nuclear antigen effect on the DNA anti-DNA reaction and NZB/NZW mouse nephritis. Extractable nuclear antigen (ENA) is composed of at least two components, one a ribonucleo-protein sensitive to ribonuclease or heat and the other a protein. Antibodies to ENA are associated with a relatively benign clinical course in patients with systemic lupus erythematosus (SLE) in which DNA anti DNA complexes are thought pathogenic. The effect of ENA and anti-ENA on DNA anti-DNA reactions in vitro was studied. ENA effectively inhibited an anti-DNA hemagglutination reaction but no effect was found on binding of radioactive DNA or on the anti-hemocyanin hemagglutination reaction. The inhibitory effect was not abolished by yeast ribonuclease (RNase), heating, or DNase. Anti-ENA HAD NO EFFECT ON ANTI-DNA hemagglutination. In vivo, ENA altered the NZB/NZW mouse nephritis thought to be a model for human SLE nephritis. These results suggest the possiblity of a role for ENA in alteration of diseases due to pathogenic DNA anti-DNA complexes."}
{"id": "PMID:1079030", "title": "Cell-mediated immunity in acute and chronic hepatitis.", "content": "Peripheral lymphocytes from patients with hepatitis-B surface antigen (HBsAg)-positive and -negative acute hepatitis (AH), chronic active hepatitis (CAH), chronic persistent hepatitis (CPH), and normal controls were tested for in vitro cytotoxicity and blast transformation. Cytotoxicity was measured by chrominum (21Cr) release into the medium from 51Cr-labeled Chang liver cells after incubation for 6 h with peripheral lymphocytes at a lymphocyte target cell ratio of 200:1. Concomitant 72-h incubation studies were performed to assess thymus cell-dependent (T) lymphocyte function as measured by conccanavalin A (Con A)- stimulated incorporation of tritiated thymidine (blast transformation) and by cytotoxicity. It was found that (a) lymphocytes from patients with AH are cytotoxic to Chang liver cells compared to controls (P less than 0.001); (b) lymphocytes from patients with acute and chronic hepatitis are less cytotoxic when incubated with autologous and homologous HB2Ag-positive and -negative AH, CAH, and CPH are as cytotoxic as normal controls when stimulated with a nonspecific mitogen such as Con A; and (d) lymphocytes from patients with CAH while on prednisone therapy showed marked depression of cytotoxicity when stimulated with Con A. Thus these studies show that patients with AH have circulating T lymphocytes which are capable of causing the destruction of Chang liver cells. There is no defect in T-cell function as measured by Con A-stimulated cytotoxicity. There is a serum factor (s) in patients with acute and chronic hepatitis which inhibits spontaneous and induced lymphocyte cytotoxicity and blast transformation. Finally, prednisone treatment appears to inhibit lymphocyte cytotoxicity in patients with CAH.", "contents": "Cell-mediated immunity in acute and chronic hepatitis. Peripheral lymphocytes from patients with hepatitis-B surface antigen (HBsAg)-positive and -negative acute hepatitis (AH), chronic active hepatitis (CAH), chronic persistent hepatitis (CPH), and normal controls were tested for in vitro cytotoxicity and blast transformation. Cytotoxicity was measured by chrominum (21Cr) release into the medium from 51Cr-labeled Chang liver cells after incubation for 6 h with peripheral lymphocytes at a lymphocyte target cell ratio of 200:1. Concomitant 72-h incubation studies were performed to assess thymus cell-dependent (T) lymphocyte function as measured by conccanavalin A (Con A)- stimulated incorporation of tritiated thymidine (blast transformation) and by cytotoxicity. It was found that (a) lymphocytes from patients with AH are cytotoxic to Chang liver cells compared to controls (P less than 0.001); (b) lymphocytes from patients with acute and chronic hepatitis are less cytotoxic when incubated with autologous and homologous HB2Ag-positive and -negative AH, CAH, and CPH are as cytotoxic as normal controls when stimulated with a nonspecific mitogen such as Con A; and (d) lymphocytes from patients with CAH while on prednisone therapy showed marked depression of cytotoxicity when stimulated with Con A. Thus these studies show that patients with AH have circulating T lymphocytes which are capable of causing the destruction of Chang liver cells. There is no defect in T-cell function as measured by Con A-stimulated cytotoxicity. There is a serum factor (s) in patients with acute and chronic hepatitis which inhibits spontaneous and induced lymphocyte cytotoxicity and blast transformation. Finally, prednisone treatment appears to inhibit lymphocyte cytotoxicity in patients with CAH."}
{"id": "PMID:1079033", "title": "Preliminary evidence for cell-mediated immunity in cross-protection among group A arboviruses.", "content": "Unfractionated and T cell-enriched populations from spleens of mice immunized with Sinbdis virus confer cross-protection against Semliki forest virus challenge in recipient mice. Both Group A viruses are serologically related, but not by tests for cross-neutralizing antibody. The protection in mice persisted for three consecutive cell passages in uninfected mice. The results suggest that cell-mediated immunity accounts for cross-protection.", "contents": "Preliminary evidence for cell-mediated immunity in cross-protection among group A arboviruses. Unfractionated and T cell-enriched populations from spleens of mice immunized with Sinbdis virus confer cross-protection against Semliki forest virus challenge in recipient mice. Both Group A viruses are serologically related, but not by tests for cross-neutralizing antibody. The protection in mice persisted for three consecutive cell passages in uninfected mice. The results suggest that cell-mediated immunity accounts for cross-protection."}
{"id": "PMID:1079034", "title": "Regulation of antibody response in vitro. IX. Induction of secondary anti-hapten IgG antibody response by anti-immunoglobulin and enhancing soluble factor.", "content": "Attempts were made to induce antibody response of hapten-primed cells by stimulation with anti-immunoglobulin (Ig) antibody and cellfree supernatant (CFS) which contained nonspecific enhancing factor. Mesenteric lymph node cells were obtained from rabbits which were primed with dinitrophenylated ragweed antigen (DNP-Rag), and the primed cells were incubated in vitro with either anti-rabbit gamma-chain or anti-Fab antibody for 24 hr. After washing, the cells were cultured in CFS which was obtained from the culture of DNP-Ascaris (DNP-Asc)-primed lymph node cells with free carrier (Asc). The results showed that hapten-primed cells were triggered for IgG anti-DNP antibody response by the two reagents, i.e., anti-Ig antibody and CFS, both which did not share any antigenic specificity with the priming antigen (DNP-Rag). Neither anti-Ig alone nor CFS alone induced anti-hapten antibody response. Anti-Ig and CFS triggered not only hapten-primed B cells but also the other IgG-bearing (B) cells for IgG synthesis. Lymph node cells of umprimed animals were activated for IgG synthesis by the stimulation with anti-Ig followed by culture in CFS. Evidence was obtained that anti-Ig antibody has to be divalent for the activation of B cells. Stimulation of DNP-primed cells with the F(ab')2 fragment of the antibody and CFS-induced anti-hapten antibody response, whereas the Fag' fragment of the anti-Ig plus CFS failed to do so. The results suggested that bridging of cell-surface Ig receptors by multivalent ligand is the initial step of B cell activation. Another evidence for the activation of hapten-primed B cells by anti-Ig was obtained by supplemental immunization of DNP-Asc-primed animals with the Fc fragment of goat IgG. Stimulation of mesenteric lymph node cells from these animals with goat anti-rabbit-Ig alone (no CFS) resulted in the formation of anti-DNP IgG antibody. The results indicated that lymphocytes primed for goat IgG collaborated with DNP-primed IgG-B cells when the lymph node cells were stimulated with goat anti-Ig.", "contents": "Regulation of antibody response in vitro. IX. Induction of secondary anti-hapten IgG antibody response by anti-immunoglobulin and enhancing soluble factor. Attempts were made to induce antibody response of hapten-primed cells by stimulation with anti-immunoglobulin (Ig) antibody and cellfree supernatant (CFS) which contained nonspecific enhancing factor. Mesenteric lymph node cells were obtained from rabbits which were primed with dinitrophenylated ragweed antigen (DNP-Rag), and the primed cells were incubated in vitro with either anti-rabbit gamma-chain or anti-Fab antibody for 24 hr. After washing, the cells were cultured in CFS which was obtained from the culture of DNP-Ascaris (DNP-Asc)-primed lymph node cells with free carrier (Asc). The results showed that hapten-primed cells were triggered for IgG anti-DNP antibody response by the two reagents, i.e., anti-Ig antibody and CFS, both which did not share any antigenic specificity with the priming antigen (DNP-Rag). Neither anti-Ig alone nor CFS alone induced anti-hapten antibody response. Anti-Ig and CFS triggered not only hapten-primed B cells but also the other IgG-bearing (B) cells for IgG synthesis. Lymph node cells of umprimed animals were activated for IgG synthesis by the stimulation with anti-Ig followed by culture in CFS. Evidence was obtained that anti-Ig antibody has to be divalent for the activation of B cells. Stimulation of DNP-primed cells with the F(ab')2 fragment of the antibody and CFS-induced anti-hapten antibody response, whereas the Fag' fragment of the anti-Ig plus CFS failed to do so. The results suggested that bridging of cell-surface Ig receptors by multivalent ligand is the initial step of B cell activation. Another evidence for the activation of hapten-primed B cells by anti-Ig was obtained by supplemental immunization of DNP-Asc-primed animals with the Fc fragment of goat IgG. Stimulation of mesenteric lymph node cells from these animals with goat anti-rabbit-Ig alone (no CFS) resulted in the formation of anti-DNP IgG antibody. The results indicated that lymphocytes primed for goat IgG collaborated with DNP-primed IgG-B cells when the lymph node cells were stimulated with goat anti-Ig."}
{"id": "PMID:1079035", "title": "Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates.", "content": "A general approach is presented for covalently attaching carboxyl and primary amino groups to Ficoll and other high molecular weight polysaccharides in a readily controlled manner. The polysaccharides are omicron-carboxymethylated with chloroacetate in NaOH solutions. Amino groups are next introduced by monoamide formation with ethylenediamine using a water-soluble carbodiimide. Reactions are carried out in aqueous solutions; excess reactants and by-products are removed by dialysis. Three different ways for coupling the 2,4-dinitrophenyl hapten to the amino derivative of Ficoll are described. Preliminary evidence indicates that 2,4-dinitrophenyl-Ficolls, prepared by procedures developed in this study, are potent thymus-independent antigens which can specifically stimulate B cells both in vivo and in vitro. Hapten-Ficoll conjugates should be exceptionally useful for studies of B lymphocyte activation because of the ready availability of the starting material and the ease with which a wide variety of hapten derivatives may be prepared.", "contents": "Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. A general approach is presented for covalently attaching carboxyl and primary amino groups to Ficoll and other high molecular weight polysaccharides in a readily controlled manner. The polysaccharides are omicron-carboxymethylated with chloroacetate in NaOH solutions. Amino groups are next introduced by monoamide formation with ethylenediamine using a water-soluble carbodiimide. Reactions are carried out in aqueous solutions; excess reactants and by-products are removed by dialysis. Three different ways for coupling the 2,4-dinitrophenyl hapten to the amino derivative of Ficoll are described. Preliminary evidence indicates that 2,4-dinitrophenyl-Ficolls, prepared by procedures developed in this study, are potent thymus-independent antigens which can specifically stimulate B cells both in vivo and in vitro. Hapten-Ficoll conjugates should be exceptionally useful for studies of B lymphocyte activation because of the ready availability of the starting material and the ease with which a wide variety of hapten derivatives may be prepared."}
{"id": "PMID:1079036", "title": "Characterization of the lymphocyte surface receptors for Con A and PHA.", "content": "Human peripheral blood lymphocytes were iodinated by the lactoperoxidase technique, and the surface receptors for the mitogens concanavalin A (Con A) and phytohemagglutinin (PHA) were analyzed by solubilizing the membrane protein and incubating with mitogen and antimitigen antibody. The resulting precipitates were washed and analyzed by gel electrophoresis. Con A was to bind to about twice as much labeled protein as PHA. Sodium dodecyl sulfate gels showed that multiple surface membrane proteins bind to these mitogens and that the pattern of peaks of Con A receptors in generally similar to the pattern of PHA receptors. Long-term lymphoid tissue culture cells lines also show multiple receptor peaks for mitogens, indicating that the heterogeneity of receptors is not caused entirely by lymphocyte heterogeneity.", "contents": "Characterization of the lymphocyte surface receptors for Con A and PHA. Human peripheral blood lymphocytes were iodinated by the lactoperoxidase technique, and the surface receptors for the mitogens concanavalin A (Con A) and phytohemagglutinin (PHA) were analyzed by solubilizing the membrane protein and incubating with mitogen and antimitigen antibody. The resulting precipitates were washed and analyzed by gel electrophoresis. Con A was to bind to about twice as much labeled protein as PHA. Sodium dodecyl sulfate gels showed that multiple surface membrane proteins bind to these mitogens and that the pattern of peaks of Con A receptors in generally similar to the pattern of PHA receptors. Long-term lymphoid tissue culture cells lines also show multiple receptor peaks for mitogens, indicating that the heterogeneity of receptors is not caused entirely by lymphocyte heterogeneity."}
{"id": "PMID:1079037", "title": "The stimulation of immunoglobulin production in murine spleen cells by the pokeweed mitogens.", "content": "We have shown that of the five mitogens purified from the salt extracts of pokeweed, only Pa-1, the B cell mitogen, has the ability to stimulate immunoglobulin production in murine B cells in the absence of classical T cells. Furthermore, the T cell mitogens (Pa-2-Pa-5) not only are unable to stimulate DNA or immunoglobulin synthesis by B cells in the absence of T cells, but cannot stimulate immunoglobulin production by B cells even in the presence of T cells. This suggests that Pa-2-Pa-5 do not stimulate adequate helper function in T cells or that if such a helper function is stimulated, an additional stimulatory factor is required for immunoglobulin production, which is not provided for by Pa-2-Pa-5.", "contents": "The stimulation of immunoglobulin production in murine spleen cells by the pokeweed mitogens. We have shown that of the five mitogens purified from the salt extracts of pokeweed, only Pa-1, the B cell mitogen, has the ability to stimulate immunoglobulin production in murine B cells in the absence of classical T cells. Furthermore, the T cell mitogens (Pa-2-Pa-5) not only are unable to stimulate DNA or immunoglobulin synthesis by B cells in the absence of T cells, but cannot stimulate immunoglobulin production by B cells even in the presence of T cells. This suggests that Pa-2-Pa-5 do not stimulate adequate helper function in T cells or that if such a helper function is stimulated, an additional stimulatory factor is required for immunoglobulin production, which is not provided for by Pa-2-Pa-5."}
{"id": "PMID:1079038", "title": "Interaction of plant lectins with purified human lymphocyte populations: binding characteristics and kinetics of proliferation.", "content": "With six purified plant lectins as probes of cell surface structure, human T, B, and Null lymphocytes have been examined for cell surface differences. The results of binding experiments with radioiodinated lectins indicate that these three lymphocyte populations, although functionally different, bind equivalent amounts of E- and L-PHA, lentil PHA, Con A, RCA-I, and WGA. Since these lectins are capable of binding to at least four different cell surface oligosaccharides, these data indicate that human T, B, and Null lymphocytes do not differ with respect to surface content of these carbohydrate structures. In addition, all six lectins were investigated for their ability to stimulate DNA synthesis in these human lymphocyte populations. Four of these lectins (E- and L-PHA, lentil PHA, and Con A) were mitogenic for human lymphocytes, whereas RCA-I and WGA failed to stimulate significant DNA synthesis in any lymphocyte population. When incubated with the mitogenic lectins, both B and Null cells displayed a delayed peak of DNA synthesis in comparison to T cells. Thus, despite virtually identical binding characteristics, the lectin-induced mitogenic response exhibited by B and Null cells was markedly different kinetically from that of T cells.", "contents": "Interaction of plant lectins with purified human lymphocyte populations: binding characteristics and kinetics of proliferation. With six purified plant lectins as probes of cell surface structure, human T, B, and Null lymphocytes have been examined for cell surface differences. The results of binding experiments with radioiodinated lectins indicate that these three lymphocyte populations, although functionally different, bind equivalent amounts of E- and L-PHA, lentil PHA, Con A, RCA-I, and WGA. Since these lectins are capable of binding to at least four different cell surface oligosaccharides, these data indicate that human T, B, and Null lymphocytes do not differ with respect to surface content of these carbohydrate structures. In addition, all six lectins were investigated for their ability to stimulate DNA synthesis in these human lymphocyte populations. Four of these lectins (E- and L-PHA, lentil PHA, and Con A) were mitogenic for human lymphocytes, whereas RCA-I and WGA failed to stimulate significant DNA synthesis in any lymphocyte population. When incubated with the mitogenic lectins, both B and Null cells displayed a delayed peak of DNA synthesis in comparison to T cells. Thus, despite virtually identical binding characteristics, the lectin-induced mitogenic response exhibited by B and Null cells was markedly different kinetically from that of T cells."}
{"id": "PMID:1079039", "title": "Quantitative and immunologic aspects of the handling of 2,4 dinitrophenyl guinea pig albumin by macrophages.", "content": "Studies correlating quantitative aspects of the handling of dinitrophenyl guinea pig albumin (DNP-GPA) by guinea pig macrophages with the potential of cell-associated antigen to initiate proliferation of immune T lymphocytes have examined the nature of immunologically relevant antigen. After the loss of 75% of cell-bound DNP-GPA during the first 24 hr of in vitro culture, the remaining antigen persists qualitatively unchanged throughout further culture. However, coincident immunogenicity of the macrophage-associated NDP-GPA progressively deccreases, suggesting loss of accessibility of the antigen to responding immune lymphocytes. There is a small, stable, surface antigen pool but these studies suggest that the immunologically critical fraction of DNP-GPA, as regards guinea pig T cell activation, is resistant to trypsinization and inaccessible to antibody.", "contents": "Quantitative and immunologic aspects of the handling of 2,4 dinitrophenyl guinea pig albumin by macrophages. Studies correlating quantitative aspects of the handling of dinitrophenyl guinea pig albumin (DNP-GPA) by guinea pig macrophages with the potential of cell-associated antigen to initiate proliferation of immune T lymphocytes have examined the nature of immunologically relevant antigen. After the loss of 75% of cell-bound DNP-GPA during the first 24 hr of in vitro culture, the remaining antigen persists qualitatively unchanged throughout further culture. However, coincident immunogenicity of the macrophage-associated NDP-GPA progressively deccreases, suggesting loss of accessibility of the antigen to responding immune lymphocytes. There is a small, stable, surface antigen pool but these studies suggest that the immunologically critical fraction of DNP-GPA, as regards guinea pig T cell activation, is resistant to trypsinization and inaccessible to antibody."}
{"id": "PMID:1079040", "title": "Alkaline phosphatase in differentiating guinea pid thymocytes: an ultracytochemical study.", "content": "Alkaline phosphatase (AP) was demonstrated ultracytochemically on the cell membrane of guinea pig thymocytes. AP usually covers the whole cell surface. In a small subpopulation of thymocytes the cell membrane is totally AP-negative. These cells are located by light microscopy in the thymic medulla and have been shown previously to possess the migratory properties of mature thymocytes. This AP-negative subpopulation most probably represents the T cell population in the thymic medulla. No distinct morphologic differences could be observed between these AP-positive and AP-negative thymocytes. Additionally, a small AP-negative cell population with characteristics of immature lymphoid cells was noted. These lymphoid cells, which are seen more numerously in the fetal thymus, might represent the differentiating stem cells in the guinea pig thymus.", "contents": "Alkaline phosphatase in differentiating guinea pid thymocytes: an ultracytochemical study. Alkaline phosphatase (AP) was demonstrated ultracytochemically on the cell membrane of guinea pig thymocytes. AP usually covers the whole cell surface. In a small subpopulation of thymocytes the cell membrane is totally AP-negative. These cells are located by light microscopy in the thymic medulla and have been shown previously to possess the migratory properties of mature thymocytes. This AP-negative subpopulation most probably represents the T cell population in the thymic medulla. No distinct morphologic differences could be observed between these AP-positive and AP-negative thymocytes. Additionally, a small AP-negative cell population with characteristics of immature lymphoid cells was noted. These lymphoid cells, which are seen more numerously in the fetal thymus, might represent the differentiating stem cells in the guinea pig thymus."}
{"id": "PMID:1079041", "title": "Release of mitogenic factor by mouse lymph node cells stimulated with PHA in vitro. Inhibition of this phenomenon by the addition of thymocytes.", "content": "Thymidine uptakes of mouse lymph node cells and thymocytes in response to phytomitogen lectins were investigated in vitro. It was observed that mixtures of lymph node cells and thymocytes yielded higher 3H-TdR incorporations than expected when exposed to concanavalin A (Con A) or pokeweed mitogen (PWM), but lower incorporations than anticipated with phytohemagglutinin (PHA) as a stimulant. The observed enhancement of the response could be explained by factors which are stimulatory for thymocytes released by the lymph node cells. However, such mitogenic factors (MF) were released not only by Con A and PWM exposed cells but also by PHA expose lymph node cells. Further experiments showed that the admixture of thymocytes inhibited the release of MF by lymph node cells exposed to PHA but not to Con A or PWM. Since medullary thymocytes did not exhibit any inhibitory activity, it is likely that cortical thymocytes are responsible for this effect. The mechanism by which thymocytes inhibit MF production of lymph node cells exposed to PHA is not clear. MF was efficiently produced by lymph node cells cultured with PHA in \"thymocyte-conditioned\" medium and the MF activity was only marginally decreased by absorption with thymocytes. The results are discussed in the light of recent findings showing that thymocytes may both enhance and depress the immune response of other lymphocytes.", "contents": "Release of mitogenic factor by mouse lymph node cells stimulated with PHA in vitro. Inhibition of this phenomenon by the addition of thymocytes. Thymidine uptakes of mouse lymph node cells and thymocytes in response to phytomitogen lectins were investigated in vitro. It was observed that mixtures of lymph node cells and thymocytes yielded higher 3H-TdR incorporations than expected when exposed to concanavalin A (Con A) or pokeweed mitogen (PWM), but lower incorporations than anticipated with phytohemagglutinin (PHA) as a stimulant. The observed enhancement of the response could be explained by factors which are stimulatory for thymocytes released by the lymph node cells. However, such mitogenic factors (MF) were released not only by Con A and PWM exposed cells but also by PHA expose lymph node cells. Further experiments showed that the admixture of thymocytes inhibited the release of MF by lymph node cells exposed to PHA but not to Con A or PWM. Since medullary thymocytes did not exhibit any inhibitory activity, it is likely that cortical thymocytes are responsible for this effect. The mechanism by which thymocytes inhibit MF production of lymph node cells exposed to PHA is not clear. MF was efficiently produced by lymph node cells cultured with PHA in \"thymocyte-conditioned\" medium and the MF activity was only marginally decreased by absorption with thymocytes. The results are discussed in the light of recent findings showing that thymocytes may both enhance and depress the immune response of other lymphocytes."}
{"id": "PMID:1079042", "title": "Characterization of mouse cells releasing or responding to mitogenic factor induced by phytomitogens in vitro.", "content": "Mouse lymphocyte populations exposed to mitogen were tested for their capacity to release factors that stimulate other lymphocytes to synthesize DNA or enhance their response to mitogens in vitro. The target lymphocyte for this mitogenic factor(s) (MF) was also characterized. The results showed that lymph node and spleen cells release more MF than thymocytes upon exposure to Con A, PHA or PWM in vitro. The T cells were found to be largely responsible for MF production, since cell suspensions depleted of phagocytic cells did not exhibit any decreased ability to produce MF and spleen cells from congenitally athymic (nude) mice produced no detectable MF activity. The MF stimulated thymocytes, lymph node cells, and spleen cells to synthesize DNA. Spleen cells from nude mice were also stimulated. The MF released by lymphocytes in response to Con A was found to induce DNA synthesis of lymphocytes in itself and did not require the presence of mitogen. It is concluded that phytomitogen lectins stimulate T cells to synthesize DNA and to release soluble factor(s) which are mitogenic for both T and B cells. The latter cells may thus be unresponsive to the phytomitogen, but still undergo blast transformation.", "contents": "Characterization of mouse cells releasing or responding to mitogenic factor induced by phytomitogens in vitro. Mouse lymphocyte populations exposed to mitogen were tested for their capacity to release factors that stimulate other lymphocytes to synthesize DNA or enhance their response to mitogens in vitro. The target lymphocyte for this mitogenic factor(s) (MF) was also characterized. The results showed that lymph node and spleen cells release more MF than thymocytes upon exposure to Con A, PHA or PWM in vitro. The T cells were found to be largely responsible for MF production, since cell suspensions depleted of phagocytic cells did not exhibit any decreased ability to produce MF and spleen cells from congenitally athymic (nude) mice produced no detectable MF activity. The MF stimulated thymocytes, lymph node cells, and spleen cells to synthesize DNA. Spleen cells from nude mice were also stimulated. The MF released by lymphocytes in response to Con A was found to induce DNA synthesis of lymphocytes in itself and did not require the presence of mitogen. It is concluded that phytomitogen lectins stimulate T cells to synthesize DNA and to release soluble factor(s) which are mitogenic for both T and B cells. The latter cells may thus be unresponsive to the phytomitogen, but still undergo blast transformation."}
{"id": "PMID:1079047", "title": "Complications of free grafts of masticatory mucosa.", "content": "A discussion has been presented and examples provided to illustrate some of the complications that may be encountered in conjunction with free grafts of masticatory mucosa. The clinical problems presented are seen infrequently, but the clinician must be aware of the possibility of their occurrence when counseling patients on the possible sequelae of such surgical procedures. A knowledge of complications that may occur in free soft tissue grafting procedures, and of the underlying mechanisms causing these problems, may aid the clinician in anticipating and avoiding similar experiences.", "contents": "Complications of free grafts of masticatory mucosa. A discussion has been presented and examples provided to illustrate some of the complications that may be encountered in conjunction with free grafts of masticatory mucosa. The clinical problems presented are seen infrequently, but the clinician must be aware of the possibility of their occurrence when counseling patients on the possible sequelae of such surgical procedures. A knowledge of complications that may occur in free soft tissue grafting procedures, and of the underlying mechanisms causing these problems, may aid the clinician in anticipating and avoiding similar experiences."}
{"id": "PMID:1079048", "title": "Effects of prenylamine on cardiac membrane currents and contractility.", "content": "The influence of prenylamine on the electrical and mechanical activity of frog atrial muscle fibers has been studied under voltage clamp conditions. At a concentration of 10-4 M, prenylamine blocks the action potential without much affecting the resting potential. The drug depresses the peak transient sodium conductance with a dissociation constant of 1.7 times 10-5 M and on a one-to-one stoichiometric basis. The curve relating peak sodium conductance to membrane potential is slightly shifted in the direction of hyperpolarization. The time to peak sodium current and the rate of sodium inactivation are not significantly altered. With 2 times 10-5 M prenylamine, the steady-state sodium inactivation curve is shifted by 5 mV to more negative membrane potentials but the decreased availability of the sodium system at the resting level is not sufficient to account for the reduction of sodium current. Recovery from sodium inactivation upon repolarization is distinctly slowed. The slow (secondary) inward current carried by calcium and/or sodium and the steady-state outward current are also depressed by prenylamine. The phasic (twitch-like) contraction related to the slow inward current is slightly decreased. The tonic (sustained) contraction associated with long-lasting depolarizations is increased and the time course of relaxation is retarded by prenylamine.", "contents": "Effects of prenylamine on cardiac membrane currents and contractility. The influence of prenylamine on the electrical and mechanical activity of frog atrial muscle fibers has been studied under voltage clamp conditions. At a concentration of 10-4 M, prenylamine blocks the action potential without much affecting the resting potential. The drug depresses the peak transient sodium conductance with a dissociation constant of 1.7 times 10-5 M and on a one-to-one stoichiometric basis. The curve relating peak sodium conductance to membrane potential is slightly shifted in the direction of hyperpolarization. The time to peak sodium current and the rate of sodium inactivation are not significantly altered. With 2 times 10-5 M prenylamine, the steady-state sodium inactivation curve is shifted by 5 mV to more negative membrane potentials but the decreased availability of the sodium system at the resting level is not sufficient to account for the reduction of sodium current. Recovery from sodium inactivation upon repolarization is distinctly slowed. The slow (secondary) inward current carried by calcium and/or sodium and the steady-state outward current are also depressed by prenylamine. The phasic (twitch-like) contraction related to the slow inward current is slightly decreased. The tonic (sustained) contraction associated with long-lasting depolarizations is increased and the time course of relaxation is retarded by prenylamine."}
{"id": "PMID:1079049", "title": "Light scattering associated with tension changes in the short-range elastic component of resting frog's muscle.", "content": "1. A study has been made of some optical and associated mechanical properties of resting frog's sartorius muscles in isotonic and hypertonic solutions. Tension and transparency changes accompanying small alterations of muscle length (smaller than 1-5%) were recorded simultaneously. 2. The form of the transparency change is complex. It has three phases, two of which occur during the length change and the third (delayed phase) after it is complete. Directional recording of the response reveals both scattering and diffraction components. 3. The change of light scattering is associated with tension changes in the short-range elastic component (SREC) of the muscle. Its magnitude is related to the stiffness of the SREC; both increase when the osmotic strength of the external solution is raised and when muscle length is increased. 4. The change of light scattering is not much affected by the velocity of the applied length change. This is true also of the mechanical stiffness of the SREC. 5. The origin of the diffraction change is not known. 6. It is concluded that the scattering effect is caused by conformational changes in the SREC.", "contents": "Light scattering associated with tension changes in the short-range elastic component of resting frog's muscle. 1. A study has been made of some optical and associated mechanical properties of resting frog's sartorius muscles in isotonic and hypertonic solutions. Tension and transparency changes accompanying small alterations of muscle length (smaller than 1-5%) were recorded simultaneously. 2. The form of the transparency change is complex. It has three phases, two of which occur during the length change and the third (delayed phase) after it is complete. Directional recording of the response reveals both scattering and diffraction components. 3. The change of light scattering is associated with tension changes in the short-range elastic component (SREC) of the muscle. Its magnitude is related to the stiffness of the SREC; both increase when the osmotic strength of the external solution is raised and when muscle length is increased. 4. The change of light scattering is not much affected by the velocity of the applied length change. This is true also of the mechanical stiffness of the SREC. 5. The origin of the diffraction change is not known. 6. It is concluded that the scattering effect is caused by conformational changes in the SREC."}
{"id": "PMID:1079053", "title": "Homologs of dopa, alpha-methyldopa, and dopamine as potential cardiovascular drugs.", "content": "Starting from 3,4-dimethoxyphenacyl bromide, 2-amino-4-(3,4-dihydroxyphenyl)butyric acid (homodopa) was synthesized in six steps. 5-Hydroxyhomodopa was similarly prepared. alpha-Methylhomodopa was synthesized in four steps from zingerone [4-(4-hydroxy-3-methoxyphenyl)-2-butanone]. alpha-Methylhomodopa showed no antihypertensive activity in the genetic hypertensive rat. Homodopa did not potentiate the behavioral effect of Dopa or inhibit Dopa decarboxylase. Homodopamine, unlike dopamine, did not increase renal blood flow in the dog.", "contents": "Homologs of dopa, alpha-methyldopa, and dopamine as potential cardiovascular drugs. Starting from 3,4-dimethoxyphenacyl bromide, 2-amino-4-(3,4-dihydroxyphenyl)butyric acid (homodopa) was synthesized in six steps. 5-Hydroxyhomodopa was similarly prepared. alpha-Methylhomodopa was synthesized in four steps from zingerone [4-(4-hydroxy-3-methoxyphenyl)-2-butanone]. alpha-Methylhomodopa showed no antihypertensive activity in the genetic hypertensive rat. Homodopa did not potentiate the behavioral effect of Dopa or inhibit Dopa decarboxylase. Homodopamine, unlike dopamine, did not increase renal blood flow in the dog."}
{"id": "PMID:1079055", "title": "In vitro studies of axillary lymph node cells in patients with breast cancer.", "content": "A total of 170 axillary lymph nodes were obtained from fresh mastectomy specimens from 81 women with breast cancer. Lymph node cells were tested in vitro for T and B cells by the rosette technique and immunofluorescence microscopy and for functional capacity by response to the mitogens phytohemagglutinin (PHA) and concanavalin A. T cells showed a wide range of relative values: 32-80 percent, with a mean of 63.5 percent. B cells defined by the presence of surface immunoglobulins ranged from 14 to 61 percent (mean, 35.8 percent); those defined by the presence of C3 receptors, from 8 to 54 percent (mean, 24.9 percent); and those defined by the presence of IgG-specific (Fc) receptors, from 10 to 45 percent (mean, 27.5 percent). Cells with the C3 and Fc receptors constituted approximately two-thirds of the cells not binding spontaneously to sheep red blood cells (non-SRBC-R), whereas virtually all non-SRBC-R stained for surface immunoglobulins. The proportion of T and B cells and the response to mitogens varied widely among nodes and among patients. Differences were significant between values observed in young and old patients, nodes with and those without metastatic disease, and lymph nodes with different morphology. Lymph nodes from patients over 60 years old showed a higher proportion of B cells and a lower proportion of T cells than did those from patients 45 years of age or younger. Lymph nodes with disease metastic to them also showed a higher percent of B cells and a lower percent of T cells than the nodes that did not have metastatic disease. Lymph nodes with lymphocyte predominance showed a relatively high proportion of T lymphocytes, a high PHA response, and a low content of B lymphocytes. By contrast, lymph nodes with germinal-center predominance showed a relatively low content of T cells, a low PHA response, and a relatively high proportion of B lymphocytes.", "contents": "In vitro studies of axillary lymph node cells in patients with breast cancer. A total of 170 axillary lymph nodes were obtained from fresh mastectomy specimens from 81 women with breast cancer. Lymph node cells were tested in vitro for T and B cells by the rosette technique and immunofluorescence microscopy and for functional capacity by response to the mitogens phytohemagglutinin (PHA) and concanavalin A. T cells showed a wide range of relative values: 32-80 percent, with a mean of 63.5 percent. B cells defined by the presence of surface immunoglobulins ranged from 14 to 61 percent (mean, 35.8 percent); those defined by the presence of C3 receptors, from 8 to 54 percent (mean, 24.9 percent); and those defined by the presence of IgG-specific (Fc) receptors, from 10 to 45 percent (mean, 27.5 percent). Cells with the C3 and Fc receptors constituted approximately two-thirds of the cells not binding spontaneously to sheep red blood cells (non-SRBC-R), whereas virtually all non-SRBC-R stained for surface immunoglobulins. The proportion of T and B cells and the response to mitogens varied widely among nodes and among patients. Differences were significant between values observed in young and old patients, nodes with and those without metastatic disease, and lymph nodes with different morphology. Lymph nodes from patients over 60 years old showed a higher proportion of B cells and a lower proportion of T cells than did those from patients 45 years of age or younger. Lymph nodes with disease metastic to them also showed a higher percent of B cells and a lower percent of T cells than the nodes that did not have metastatic disease. Lymph nodes with lymphocyte predominance showed a relatively high proportion of T lymphocytes, a high PHA response, and a low content of B lymphocytes. By contrast, lymph nodes with germinal-center predominance showed a relatively low content of T cells, a low PHA response, and a relatively high proportion of B lymphocytes."}
{"id": "PMID:1079056", "title": "Immunogenetics of a thymus antigen in lymphoma-prone and lymphoma-resistant colonies of wild mice.", "content": "The Thy-1 (theta) antigen was identified in wild mice (Mus musculus), the frequency of its alleles was determined in two natural populations of wild mice, and the possible T-cell origin of spontaneous lymphomas was investigated in one of these populations. Reaction patterns for Thy-1 antigen with the use of direct cytoxicity and indirect absorption assays were identical in wild mice and inbred strains. Between 15 and 55 percent of viable spleen cells from healthy young or old wild mice were Thy-1 positive. Spleen, but not brain, cells from older wild mice were less strongly positive. Wild mice from the lymphoma-prone population were polymorphic for Thy-1alpha and Thy-1beta alleles, whereas wild mice from the lymphoma-resistant populations were homozygous for the Thy-1beta allele. Contrary to expectation, a higher frequency of the Thy-1beta allele was noted in the mice with lymphomas. Alleles at two other loci on chromosome number 9 (Mod-1alpha and Trfbeta) were fixed in both populations. The absence of detectable Thy-1 antigen on spleen cells (despite its detectability in undiminished titer in brain tissue) in 80 percent of mice with lymphomas, along with the absence of thymus involvement in the lymphomatous proliferations, suggested that these tumors are derived from an expansion of non-thymus-derived cells.", "contents": "Immunogenetics of a thymus antigen in lymphoma-prone and lymphoma-resistant colonies of wild mice. The Thy-1 (theta) antigen was identified in wild mice (Mus musculus), the frequency of its alleles was determined in two natural populations of wild mice, and the possible T-cell origin of spontaneous lymphomas was investigated in one of these populations. Reaction patterns for Thy-1 antigen with the use of direct cytoxicity and indirect absorption assays were identical in wild mice and inbred strains. Between 15 and 55 percent of viable spleen cells from healthy young or old wild mice were Thy-1 positive. Spleen, but not brain, cells from older wild mice were less strongly positive. Wild mice from the lymphoma-prone population were polymorphic for Thy-1alpha and Thy-1beta alleles, whereas wild mice from the lymphoma-resistant populations were homozygous for the Thy-1beta allele. Contrary to expectation, a higher frequency of the Thy-1beta allele was noted in the mice with lymphomas. Alleles at two other loci on chromosome number 9 (Mod-1alpha and Trfbeta) were fixed in both populations. The absence of detectable Thy-1 antigen on spleen cells (despite its detectability in undiminished titer in brain tissue) in 80 percent of mice with lymphomas, along with the absence of thymus involvement in the lymphomatous proliferations, suggested that these tumors are derived from an expansion of non-thymus-derived cells."}
{"id": "PMID:1079060", "title": "Acute duodenitis and duodenal ulceration after burns. Clinical and pathological characteristics.", "content": "The clinical and pathological characteristics of Curling ulcer were defined by early and serial endoscopic examination of the duodenum in 37 burned patients. Duodenal disease was present in 27 patients and occurred only in patients with burns involving more than 38 percent of the total body surface. Erosive \"duodenitis\" could occur within 12 hours after injury and was usually associated with acute gastric disease. Isolated duodenitis occurred only in patients with pancreatitis. Contrast roentgenograms did not reliably show the superficial mucosal disease. Duodenal ulcerations were present in 12 patients and developed on a background of diffuse superficial mucosal injury. Other complications in the patient's postburn course influenced disease progression. Hemorrhage occurred in six patients with duodenal disease, usually originating from a posterior duodenal ulcer. Uncomplicated ulcers invariably healed within five weeks after diagnosis.", "contents": "Acute duodenitis and duodenal ulceration after burns. Clinical and pathological characteristics. The clinical and pathological characteristics of Curling ulcer were defined by early and serial endoscopic examination of the duodenum in 37 burned patients. Duodenal disease was present in 27 patients and occurred only in patients with burns involving more than 38 percent of the total body surface. Erosive \"duodenitis\" could occur within 12 hours after injury and was usually associated with acute gastric disease. Isolated duodenitis occurred only in patients with pancreatitis. Contrast roentgenograms did not reliably show the superficial mucosal disease. Duodenal ulcerations were present in 12 patients and developed on a background of diffuse superficial mucosal injury. Other complications in the patient's postburn course influenced disease progression. Hemorrhage occurred in six patients with duodenal disease, usually originating from a posterior duodenal ulcer. Uncomplicated ulcers invariably healed within five weeks after diagnosis."}
{"id": "PMID:1079061", "title": "Angina during hemodialysis. Treatment by coronary bypass graft.", "content": "In a 52-year-old man with chronic renal failure, severe angina occured when he was receiving hemodialysis. A double coronary bypass graft was performed, providing complete relief.", "contents": "Angina during hemodialysis. Treatment by coronary bypass graft. In a 52-year-old man with chronic renal failure, severe angina occured when he was receiving hemodialysis. A double coronary bypass graft was performed, providing complete relief."}
{"id": "PMID:1079063", "title": "Use of blood component therapy for gastrointestinal bleeding in patients with cirrhosis of the liver.", "content": "A prospective study was designed to compare the administration of available fresh blood and component therapy in the treatment of gastrointestinal bleeding in patients with cirrhosis of the liver. Fifty bleeding cirrhotic patients were randomly assigned to treatment: 17 patients recieved 51 percent fresh blood (Group 1), 16 patients received 22 percent fresh blood (Group 2), and 17 patients received 25 percent component therapy consisting of packed cells, fresh frozen plasma, and platelet concentrate (Group 3).  The mortality rate was unaffected by the type of blood replacement.  Neither the blood replacement requirement (Group 1:51 plus or minus 0.9 units Group 2:5.5 plus or minus 0.8 units, Group 3:7.1 plus or minus 1.1 units) nor the duration of bleeding (Group 1:39 plus or minus 0.8 days, Group 2:6.3 plus or minus 2.4 days, Groups 3:5.8 plus or minus 1.0 days) were significantly different.  There was no correlation between the mean age of blood or plasma received and the units of blood replaced or the duration of bleeding.  Patients with severe coagulation abnormalties had a significantly increased mortality.  Component therapy was as effective as the fresh blood regimen in cirrhotic patients with acute gastrintestinal hemorrhage.", "contents": "Use of blood component therapy for gastrointestinal bleeding in patients with cirrhosis of the liver. A prospective study was designed to compare the administration of available fresh blood and component therapy in the treatment of gastrointestinal bleeding in patients with cirrhosis of the liver. Fifty bleeding cirrhotic patients were randomly assigned to treatment: 17 patients recieved 51 percent fresh blood (Group 1), 16 patients received 22 percent fresh blood (Group 2), and 17 patients received 25 percent component therapy consisting of packed cells, fresh frozen plasma, and platelet concentrate (Group 3).  The mortality rate was unaffected by the type of blood replacement.  Neither the blood replacement requirement (Group 1:51 plus or minus 0.9 units Group 2:5.5 plus or minus 0.8 units, Group 3:7.1 plus or minus 1.1 units) nor the duration of bleeding (Group 1:39 plus or minus 0.8 days, Group 2:6.3 plus or minus 2.4 days, Groups 3:5.8 plus or minus 1.0 days) were significantly different.  There was no correlation between the mean age of blood or plasma received and the units of blood replaced or the duration of bleeding.  Patients with severe coagulation abnormalties had a significantly increased mortality.  Component therapy was as effective as the fresh blood regimen in cirrhotic patients with acute gastrintestinal hemorrhage."}
{"id": "PMID:1079064", "title": "[Effect of an increased content of oxygen and carbon dioxide on vestibular resistance].", "content": "Forty-two experiments on 21 test subjects, aged 20 to 40, were carried out to study possibilities of increasing vestibular resistance. This was achieved by improving the cerebral blood supply of the subjects who used the breathing air of 40-43% oxygen, 2% carbon dioxide and nitrogen. The results were treated statistically. This breathing air proved to increase the resistance of the vestibular analyzer to adequate stimuli. It is recommended to use this breathing air consisting of 40-43% oxygen, 2% carbon dioxide and nitrogen to prevent motion sickness and to discontinue vestibulo-vegetative disturbances that may appear in people using different flying and ground vehicles.", "contents": "[Effect of an increased content of oxygen and carbon dioxide on vestibular resistance]. Forty-two experiments on 21 test subjects, aged 20 to 40, were carried out to study possibilities of increasing vestibular resistance. This was achieved by improving the cerebral blood supply of the subjects who used the breathing air of 40-43% oxygen, 2% carbon dioxide and nitrogen. The results were treated statistically. This breathing air proved to increase the resistance of the vestibular analyzer to adequate stimuli. It is recommended to use this breathing air consisting of 40-43% oxygen, 2% carbon dioxide and nitrogen to prevent motion sickness and to discontinue vestibulo-vegetative disturbances that may appear in people using different flying and ground vehicles."}
{"id": "PMID:1079067", "title": "Early cellular responses to mitogens and adjuvants in the mouse spleen.", "content": "The purpose of this study was to evaluate the cellular events in the spleens of mice following the intravenous injection of mitogens and adjuvants. The compounds used were concanavalin A, polyadenylic polyuridylic acid, beryllium sulfate, bacterial endotoxin, tuberculin-purified protein derivative, and dextran sulfate. Nine different strains of mice (some deficient in the C5 component of complement) received a single dose of these compounds, and their spleens were studied at sequential time intervals, ranging from 1 hour to 14 days. Concanavalin A triggered marked blast activity in the T cell zones of the splenic white pulp which was maximal at 24 hours following the injection. -3H-thymidine incorporation increased significantly, but the number of immunoglobulin-negative cells did not increase, probably because of a concomitant loss of hematopoietic cells. Polyadenylic polyuridylic acid and beryllium sulfate produced an increase in the number of lymphocytes in the T cell zones by 12 to 24 hours, but mitotic activity was unremarkable. None of the above T cell zone changes was observed in neonatally thymectomized mice. Endotoxin, purified protein derivative, and dextran sulfate produced marked B cell zone hyperplasia. Similar histologic changes were seen in the thymectomized animals. -3H-thymidine incorporation and number of immunoglobulin-positive cells were significantly increased by 24 hours. Endotoxin and dextran sulfate in some strains of mice caused marked depletion of the T cell zones. The effects of concanavalin A, endotoxin, and dextran sulfate were unrelated to the presence or absence of C5 protein. These experiments show that (1) concanavalin A, bacterial endotoxin, tuberulin-purified protein derivative, and dextran sulfate trigger in vivo the same cellular components of the immune system as they do in vitro; (2) polyadenylic polyuridylic acid and beryllium sulfate may influence the immune system by increased localization of lymphocytes in the T cell zones.", "contents": "Early cellular responses to mitogens and adjuvants in the mouse spleen. The purpose of this study was to evaluate the cellular events in the spleens of mice following the intravenous injection of mitogens and adjuvants. The compounds used were concanavalin A, polyadenylic polyuridylic acid, beryllium sulfate, bacterial endotoxin, tuberculin-purified protein derivative, and dextran sulfate. Nine different strains of mice (some deficient in the C5 component of complement) received a single dose of these compounds, and their spleens were studied at sequential time intervals, ranging from 1 hour to 14 days. Concanavalin A triggered marked blast activity in the T cell zones of the splenic white pulp which was maximal at 24 hours following the injection. -3H-thymidine incorporation increased significantly, but the number of immunoglobulin-negative cells did not increase, probably because of a concomitant loss of hematopoietic cells. Polyadenylic polyuridylic acid and beryllium sulfate produced an increase in the number of lymphocytes in the T cell zones by 12 to 24 hours, but mitotic activity was unremarkable. None of the above T cell zone changes was observed in neonatally thymectomized mice. Endotoxin, purified protein derivative, and dextran sulfate produced marked B cell zone hyperplasia. Similar histologic changes were seen in the thymectomized animals. -3H-thymidine incorporation and number of immunoglobulin-positive cells were significantly increased by 24 hours. Endotoxin and dextran sulfate in some strains of mice caused marked depletion of the T cell zones. The effects of concanavalin A, endotoxin, and dextran sulfate were unrelated to the presence or absence of C5 protein. These experiments show that (1) concanavalin A, bacterial endotoxin, tuberulin-purified protein derivative, and dextran sulfate trigger in vivo the same cellular components of the immune system as they do in vitro; (2) polyadenylic polyuridylic acid and beryllium sulfate may influence the immune system by increased localization of lymphocytes in the T cell zones."}
{"id": "PMID:1079068", "title": "Deoxyribonucleic acid synthetic response of mouse lymphoid cells in microculture with T-lymphocyte-dependent mitogens.", "content": "Various numbers of mouse thymus, lymph node, or spleen cells were cultured in serum-containing medium with concentrations of 0.06 to 64 mug. per ml. of concanavalin A (Con A), using our version of the microculture technique. Maximal DNA synthetic responses were obtained with 1.0 million thymocytes (highest number used) at 4 mug. per ml. of Con A and with 0.75 million lymph node cells or 0.5 million spleen cells at 1 mug. per ml. of Con A. At optimal Con A concentration the magnitudes of response of 0.25 million thymus, compared with the same number of lymph node cells, were very similar. However, the response of lymph node cells was superior to that of thymocytes when 0.5 million initial cells were used, whereas 1.0 million thymocytes gave a better response than did the same number of lymph node cells. Regardless of the cell concentration, a comparison of responses by the three different cell types with the same cell number showed that the Con A concentration-DNA synthetic response curve was broadest for spleen cells, narrow for thymus cells, and intermediate for lymph node cells. Results in replicate experiments were highly reproducible, and the data indicated that peak DNA synthetic responses were not directly proportional to cell number when certain numbers of cells were used; the actual proportionality of response to cell number varied with the cell type and the mitogen concentration. When examined throughout the culture period, concentrations of Con A which were above the optimal for stimulation of DNA synthesis resulted in significant decreases in the number of viable cells. Time course experiments with Con A (or phytohemagglutinin)-stimulated BALB/c spleen cells showed that the initiation of DNA synthesis occurred between 12 and 24 hours of culture, with peak levels of 3H-thymidine incorporation occurring during the 2nd and 3rd days of culture. The presence or absence of fetal calf serum had essentially no effect on the Con A- or phytohemagglutinin-stimulated DNA synthetic responses during the 3rd day of culture. These results demonstrate that detailed evaluation of culture variables and mitogen concentrations is required for the most meaningful use of mitogen-stimulated DNA synthesis as a parameter for comparing different lymphoid cell populations.", "contents": "Deoxyribonucleic acid synthetic response of mouse lymphoid cells in microculture with T-lymphocyte-dependent mitogens. Various numbers of mouse thymus, lymph node, or spleen cells were cultured in serum-containing medium with concentrations of 0.06 to 64 mug. per ml. of concanavalin A (Con A), using our version of the microculture technique. Maximal DNA synthetic responses were obtained with 1.0 million thymocytes (highest number used) at 4 mug. per ml. of Con A and with 0.75 million lymph node cells or 0.5 million spleen cells at 1 mug. per ml. of Con A. At optimal Con A concentration the magnitudes of response of 0.25 million thymus, compared with the same number of lymph node cells, were very similar. However, the response of lymph node cells was superior to that of thymocytes when 0.5 million initial cells were used, whereas 1.0 million thymocytes gave a better response than did the same number of lymph node cells. Regardless of the cell concentration, a comparison of responses by the three different cell types with the same cell number showed that the Con A concentration-DNA synthetic response curve was broadest for spleen cells, narrow for thymus cells, and intermediate for lymph node cells. Results in replicate experiments were highly reproducible, and the data indicated that peak DNA synthetic responses were not directly proportional to cell number when certain numbers of cells were used; the actual proportionality of response to cell number varied with the cell type and the mitogen concentration. When examined throughout the culture period, concentrations of Con A which were above the optimal for stimulation of DNA synthesis resulted in significant decreases in the number of viable cells. Time course experiments with Con A (or phytohemagglutinin)-stimulated BALB/c spleen cells showed that the initiation of DNA synthesis occurred between 12 and 24 hours of culture, with peak levels of 3H-thymidine incorporation occurring during the 2nd and 3rd days of culture. The presence or absence of fetal calf serum had essentially no effect on the Con A- or phytohemagglutinin-stimulated DNA synthetic responses during the 3rd day of culture. These results demonstrate that detailed evaluation of culture variables and mitogen concentrations is required for the most meaningful use of mitogen-stimulated DNA synthesis as a parameter for comparing different lymphoid cell populations."}
{"id": "PMID:1079065", "title": "[Functional state of the vestibular analyzer during lower body negative pressure tests].", "content": "With the aid of the caloric test the functional state of the vestibular analyzer of nine male athletes was investigated during their exposure to lower body negative pressure tests. The prolongation of the latent period of the caloric nystagmus and vertigo, shortening of the time of the nystagmus and vertigo were observed. It is concluded that the cerebral blood supply plays a major role in the development of vestibular reflexes.", "contents": "[Functional state of the vestibular analyzer during lower body negative pressure tests]. With the aid of the caloric test the functional state of the vestibular analyzer of nine male athletes was investigated during their exposure to lower body negative pressure tests. The prolongation of the latent period of the caloric nystagmus and vertigo, shortening of the time of the nystagmus and vertigo were observed. It is concluded that the cerebral blood supply plays a major role in the development of vestibular reflexes."}
{"id": "PMID:1079072", "title": "[Ocular complications in ophthalmic zoster (author's transl)].", "content": "Ocular complications occur in about 50% of cases of ophthalmic zoster. They include inflammatory reactions of the eyelid, conjunctivitis, scleritis, keratitis, iridocyclitis, secondary glaucoma, optic neuritis, internal ophthalmoplegia, ocular motor palsies and exophthalmos. Very dangerous complications are a concomitant facial paralysis and a neuroparalytic keratitis. Then a tarsorrhaphy should be done in time. An ophthalmologist should be consulted, when the side of the tip of the nose presents vesicles (Hutchinson's rule).", "contents": "[Ocular complications in ophthalmic zoster (author's transl)]. Ocular complications occur in about 50% of cases of ophthalmic zoster. They include inflammatory reactions of the eyelid, conjunctivitis, scleritis, keratitis, iridocyclitis, secondary glaucoma, optic neuritis, internal ophthalmoplegia, ocular motor palsies and exophthalmos. Very dangerous complications are a concomitant facial paralysis and a neuroparalytic keratitis. Then a tarsorrhaphy should be done in time. An ophthalmologist should be consulted, when the side of the tip of the nose presents vesicles (Hutchinson's rule)."}
{"id": "PMID:1079076", "title": "The effect of various putative neurotransmitters on the release of corticotrophin releasing hormone from the hypothalamus of the rat in vitro. I. The effect of acetylcholine and noradrenaline.", "content": "The effect of various putative neurotransmitters upon the release of corticotrophin releasing hormone (CRH) was investigated using the hypothalamus of the rat in vitro. Acetylcholine (1-5 pg) caused a dose-dependent release of CRH which was antagonized by hexamethonium (1-10ng) and partially antagonized by atropine (300 pg). Neither noradrenaline, dopamine nor histamine had any effect on the basal secretion of CRH. Noradrenaline (10 ng), however, was able to inhibit the release of CRH in response to acetylcholine (3 pg) and this action of noradrenaline was reduced by phentolamine (100 ng), an alpha adrenergic blocking agent.", "contents": "The effect of various putative neurotransmitters on the release of corticotrophin releasing hormone from the hypothalamus of the rat in vitro. I. The effect of acetylcholine and noradrenaline. The effect of various putative neurotransmitters upon the release of corticotrophin releasing hormone (CRH) was investigated using the hypothalamus of the rat in vitro. Acetylcholine (1-5 pg) caused a dose-dependent release of CRH which was antagonized by hexamethonium (1-10ng) and partially antagonized by atropine (300 pg). Neither noradrenaline, dopamine nor histamine had any effect on the basal secretion of CRH. Noradrenaline (10 ng), however, was able to inhibit the release of CRH in response to acetylcholine (3 pg) and this action of noradrenaline was reduced by phentolamine (100 ng), an alpha adrenergic blocking agent."}
{"id": "PMID:1079077", "title": "Postpartum hemorrhage and reevaluation of uterine packing.", "content": "Postpartum hemorrhage, occurring in 153 patients, is categorized according to etiology, predisposing conditions, and severity. Ruptured uterus is considered as a cause of postpartum hemorrhage, with an incidence of 7.1% overall and 11.9% in patients with severe hemorrhage. The effectiveness and complications of the uterine pack, used in 33 patients, are evaluated.", "contents": "Postpartum hemorrhage and reevaluation of uterine packing. Postpartum hemorrhage, occurring in 153 patients, is categorized according to etiology, predisposing conditions, and severity. Ruptured uterus is considered as a cause of postpartum hemorrhage, with an incidence of 7.1% overall and 11.9% in patients with severe hemorrhage. The effectiveness and complications of the uterine pack, used in 33 patients, are evaluated."}
{"id": "PMID:1079075", "title": "[Traumatic thrombosis of cerebral arteries].", "content": "The authors report 4 cases of angiographically diagnosed traumatic thromboses of cerebral arteries. In two of them thrombosis of the middle cerebral artery was present and in one of them it was confirmed on autopsy. In one case thrombosis involved the basilar artery and in another the subcallosal artery. The time between the injury and development of neurological manifestations was from several hours to several weeks. Major disturbances of consciousness at the time of onset of focal central nervous system lesion were never observed. Attention is called to the necessity of differential diagnosis of these conditions against traumatic haematoma. In the light of pertinent literature the problems of diagnosis, treatment and pathogenesis of traumatic cerebral arterial thrombosis are discussed.", "contents": "[Traumatic thrombosis of cerebral arteries]. The authors report 4 cases of angiographically diagnosed traumatic thromboses of cerebral arteries. In two of them thrombosis of the middle cerebral artery was present and in one of them it was confirmed on autopsy. In one case thrombosis involved the basilar artery and in another the subcallosal artery. The time between the injury and development of neurological manifestations was from several hours to several weeks. Major disturbances of consciousness at the time of onset of focal central nervous system lesion were never observed. Attention is called to the necessity of differential diagnosis of these conditions against traumatic haematoma. In the light of pertinent literature the problems of diagnosis, treatment and pathogenesis of traumatic cerebral arterial thrombosis are discussed."}
{"id": "PMID:1079082", "title": "Roentgenographic evaluation of infant and childhood trauma.", "content": "The roentgenographic evaluation of the traumatized child must be appropriate for the injuries present. In the acute phase emergency screening roentgenograms tailored to the type of trauma being investigated are performed. Detailed evaluation and special studies are performed when indicated. In some instances these examinations are needed on an emergency basis; in other cases a more leisurely study can be performed when the patient's condition improves.", "contents": "Roentgenographic evaluation of infant and childhood trauma. The roentgenographic evaluation of the traumatized child must be appropriate for the injuries present. In the acute phase emergency screening roentgenograms tailored to the type of trauma being investigated are performed. Detailed evaluation and special studies are performed when indicated. In some instances these examinations are needed on an emergency basis; in other cases a more leisurely study can be performed when the patient's condition improves."}
{"id": "PMID:1079083", "title": "Spike frequency of the nodal membrane generated by high-frequency alternating current.", "content": "Changes in membrane potential of single frog motor nerve fibres due to alternating current (ac) between 4 kHz and 20 kHz were recorded in the air gap equipment under constant current conditions at 20 degrees C. The experimental findings were compared with the results of computations on the basis of potential clamp data. Ac shifted mean membrane potential (averaged for every ac period) in the direction of depolarization. The mean depolarization Vm depended on current strength I; it disappeared when the sodium permeability was blocked, in the experiments by tetrodotoxin. In a current range between about 1 and 3 fold threshold strength the ac initiated repetitive activity with response frequencies v between averaged 120 Hz and 820 Hz or in the computations even higher; v depended logarithimically on current strength, but was independent of ac frequency. Elimination of current amplitude I from the nonlinear realtions v(I)  and Vm(I) led to a linear function between v and Vm. Both v and Vm depended markedly on prepolarization of the node. The results were attributed to the preferred activation of the sodium permeability under maintained high frequency ac stimulation. Differences between computations and constant current experiments occurred for very long stimulus duration when rhythmical discharges died out in the experiment.", "contents": "Spike frequency of the nodal membrane generated by high-frequency alternating current. Changes in membrane potential of single frog motor nerve fibres due to alternating current (ac) between 4 kHz and 20 kHz were recorded in the air gap equipment under constant current conditions at 20 degrees C. The experimental findings were compared with the results of computations on the basis of potential clamp data. Ac shifted mean membrane potential (averaged for every ac period) in the direction of depolarization. The mean depolarization Vm depended on current strength I; it disappeared when the sodium permeability was blocked, in the experiments by tetrodotoxin. In a current range between about 1 and 3 fold threshold strength the ac initiated repetitive activity with response frequencies v between averaged 120 Hz and 820 Hz or in the computations even higher; v depended logarithimically on current strength, but was independent of ac frequency. Elimination of current amplitude I from the nonlinear realtions v(I)  and Vm(I) led to a linear function between v and Vm. Both v and Vm depended markedly on prepolarization of the node. The results were attributed to the preferred activation of the sodium permeability under maintained high frequency ac stimulation. Differences between computations and constant current experiments occurred for very long stimulus duration when rhythmical discharges died out in the experiment."}
{"id": "PMID:1079084", "title": "Eye, head and body movements of the guinea pig in response to optokinetic stimulation and sinusoidal oscillation in yaw.", "content": "Reflex head and eye movements were observed in the guinea pig subjected to optokinetic stimulation and whole body oscillation in the yaw axis. Optokinetic stimuli induced low velocity head movements in the direction of the stimulus with stepwise movements in the opposite direction. With motion stimuli, the head was well stabilized in space and moved to new positions in stepwise manner; in the absence of vision the head was not able to maintain a stable position. The onset of a stepwise head movement was always synchronized with a saccadic eye movement in the same direction, the coordination being similar to voluntary movement. During good head stabilization, vestibular-ocular nystagmus did not occur. The guinea pig appears to avoid nystagmus by stabilizing its head preferentially. It is likely that the vestibular ocular reflex compensates for head displacement with unity gain in the frequency range of voluntary and stepwise reflex head movement and that a close relationship exists between the control of saccadic eye movements and that of voluntary or reflex head movement.", "contents": "Eye, head and body movements of the guinea pig in response to optokinetic stimulation and sinusoidal oscillation in yaw. Reflex head and eye movements were observed in the guinea pig subjected to optokinetic stimulation and whole body oscillation in the yaw axis. Optokinetic stimuli induced low velocity head movements in the direction of the stimulus with stepwise movements in the opposite direction. With motion stimuli, the head was well stabilized in space and moved to new positions in stepwise manner; in the absence of vision the head was not able to maintain a stable position. The onset of a stepwise head movement was always synchronized with a saccadic eye movement in the same direction, the coordination being similar to voluntary movement. During good head stabilization, vestibular-ocular nystagmus did not occur. The guinea pig appears to avoid nystagmus by stabilizing its head preferentially. It is likely that the vestibular ocular reflex compensates for head displacement with unity gain in the frequency range of voluntary and stepwise reflex head movement and that a close relationship exists between the control of saccadic eye movements and that of voluntary or reflex head movement."}
{"id": "PMID:1079085", "title": "Studies on the thymic dependence of the immunoglobulin classes of the mouse (38570).", "content": "The influence of the thymus on serum immunoglobin (Ig) concentration was studied by a comparison of serum Ig levels in congenitally athymic (nu/nu) mice versus control littermate heterozygotes and adult thymectomized, irradiated, bone marrow reconsituted mice (ATx plus B) versus adult thymectomized, irradiated mice reconstituted with bone marrow and thymus (ATx plus BT). IN THE FORMER GROUP IgGl.  IgA, and IgG2a were 8%, 17% and 31% of controls.  IgM levels were increased (340%) compared to controls.  When ATx plus B mice were compared to controls.  When ATx plus B mice were compared with nonirradiated controls sigificant depressions were noted in serum IbGl and IgM.  The only significant decrease in serum Ig levels between ATx plus B and ATx plus BT was in IgGl.  These results are discussed in terms of the effects of thymic influence, residual T lymphocyte population differences between the two groups, and the effect of irradiation.", "contents": "Studies on the thymic dependence of the immunoglobulin classes of the mouse (38570). The influence of the thymus on serum immunoglobin (Ig) concentration was studied by a comparison of serum Ig levels in congenitally athymic (nu/nu) mice versus control littermate heterozygotes and adult thymectomized, irradiated, bone marrow reconsituted mice (ATx plus B) versus adult thymectomized, irradiated mice reconstituted with bone marrow and thymus (ATx plus BT). IN THE FORMER GROUP IgGl.  IgA, and IgG2a were 8%, 17% and 31% of controls.  IgM levels were increased (340%) compared to controls.  When ATx plus B mice were compared to controls.  When ATx plus B mice were compared with nonirradiated controls sigificant depressions were noted in serum IbGl and IgM.  The only significant decrease in serum Ig levels between ATx plus B and ATx plus BT was in IgGl.  These results are discussed in terms of the effects of thymic influence, residual T lymphocyte population differences between the two groups, and the effect of irradiation."}
{"id": "PMID:1079087", "title": "Potentiation of the L-5-hydroxytryptophan-induced elevation of plasma corticosterone levels in rats by a specific inhibitor of serotonin uptake.", "content": "The ability of L-5-hydroxytryptophan (L-5HTP), a serotonin precursor, to elevate plasma levels of corticosterone in rats was confirmed. Pretreatment of the rats with 3-(p-trifluoromethylphenoxy)-N-methyl-3-phenyl-propylamine hydrochloride (Lilly 110140) markedly enhanced the elevation of corticosterone levels  by L-5HTP. Since Lilly 110140 is a specific inhibitor of serotonin uptake, these results support the hypothesis that L-5HTP elevates corticosterone by enhancing neurotransmission through a serotoninergic pathway influencing ACTH release.", "contents": "Potentiation of the L-5-hydroxytryptophan-induced elevation of plasma corticosterone levels in rats by a specific inhibitor of serotonin uptake. The ability of L-5-hydroxytryptophan (L-5HTP), a serotonin precursor, to elevate plasma levels of corticosterone in rats was confirmed. Pretreatment of the rats with 3-(p-trifluoromethylphenoxy)-N-methyl-3-phenyl-propylamine hydrochloride (Lilly 110140) markedly enhanced the elevation of corticosterone levels  by L-5HTP. Since Lilly 110140 is a specific inhibitor of serotonin uptake, these results support the hypothesis that L-5HTP elevates corticosterone by enhancing neurotransmission through a serotoninergic pathway influencing ACTH release."}
{"id": "PMID:1079088", "title": "Liver volume estimated by ultrasonic scanning before and after portal decompression surgery.", "content": "In 12 patients with cirrhosis of the liver, determination of the liver volume by ultrasonic scanning and of the galactose elimination capacity (GE) were made before and after portal decompression surgery. The liver volume decreased significantly, with a median decrease of 402 ml (psmaller than 0.01). Also the GE decreased, but relatively less than the liver volume, so that the calculated GE per unit liver volume increased, with a median increase of 5 mg per minute times 100 ml liver volume (psmaller than 0.005). The decrease in liver volume following portal decompression is assumed to be due in part to reduction of liver blood volume, in part to loss of tissue water and fat, and probably to some loss of parenchyma, accounting for a reduction of the GE.", "contents": "Liver volume estimated by ultrasonic scanning before and after portal decompression surgery. In 12 patients with cirrhosis of the liver, determination of the liver volume by ultrasonic scanning and of the galactose elimination capacity (GE) were made before and after portal decompression surgery. The liver volume decreased significantly, with a median decrease of 402 ml (psmaller than 0.01). Also the GE decreased, but relatively less than the liver volume, so that the calculated GE per unit liver volume increased, with a median increase of 5 mg per minute times 100 ml liver volume (psmaller than 0.005). The decrease in liver volume following portal decompression is assumed to be due in part to reduction of liver blood volume, in part to loss of tissue water and fat, and probably to some loss of parenchyma, accounting for a reduction of the GE."}
{"id": "PMID:1079089", "title": "Preoperative liver function tests correlated with encephalopathy after porta-caval anastomosis.", "content": "In 31 patients with cirrhosis of the liver undergoing porta-caval shunt surgery, the prognostic value of some liver function tests was studied. The surgical mortality was not correlated with the test results. Serum concentrations of bilirubin, albumin, and prothrombin were not correlated with postoperative encephalopathy, but the mutually correlated preoperative galactose elimination capacity and age of patients were correlated with encephalopathy development. Incapacitating encephalopathy mainly occurred in patients above 60 years of age, and when the galactose elimination capacity was at or less  than an arbitrary limit of 225 mg/min. The liver function decreased significantly following operation.", "contents": "Preoperative liver function tests correlated with encephalopathy after porta-caval anastomosis. In 31 patients with cirrhosis of the liver undergoing porta-caval shunt surgery, the prognostic value of some liver function tests was studied. The surgical mortality was not correlated with the test results. Serum concentrations of bilirubin, albumin, and prothrombin were not correlated with postoperative encephalopathy, but the mutually correlated preoperative galactose elimination capacity and age of patients were correlated with encephalopathy development. Incapacitating encephalopathy mainly occurred in patients above 60 years of age, and when the galactose elimination capacity was at or less  than an arbitrary limit of 225 mg/min. The liver function decreased significantly following operation."}
{"id": "PMID:1079090", "title": "The use of a short-lived radionuclide in the investigation of acute bleeding in the upper gastrointestinal tract.", "content": "A method is described for the detection and monitoring of bleeding in the upper gastrointestinal tract. A short-lived radionuclide (113In-m) is used. Acute bleeding in the upper gastrointestinal tract in patients without signs of shock and preshock usually stopped within a short time after onset.", "contents": "The use of a short-lived radionuclide in the investigation of acute bleeding in the upper gastrointestinal tract. A method is described for the detection and monitoring of bleeding in the upper gastrointestinal tract. A short-lived radionuclide (113In-m) is used. Acute bleeding in the upper gastrointestinal tract in patients without signs of shock and preshock usually stopped within a short time after onset."}
{"id": "PMID:1079091", "title": "[Problems of aortocoronary bypass. Indications for coronary angiography and ventriculography; results of direct bypassing coronary surgery].", "content": "The introduction of selective coronary arteriography and aorto-to-coronary saphenous vein bypass surgery (a.-c bypass) has fundamentally altered our understanding of ischemic heart disease (IHD). The indications for the effective diagnostic procedure and the results of the new and increasingly important surgical technique are summarized. Selective coronary arteriography should be performed (a) in patients with known IHD in order to furnish the anatomical and functional information necessary to assess the indication for surgery, i.e. in patients below 60 years with intractable stable or unstable (impending infarction) angina. It is rarely indicated in patients with an old myocardial infarction who are free from symptoms. It is debatable in patients during the acute stage of infarction; (b) in patients with questionable IHD, with the aim of ruling out or confirming the condition, i.e. mainly in patients with atypical chest pain or with equivocal ecg findings. The risks of the procedure, if carried out by experienced personnel, are small. Selective arteriography will always be supplemented by a selective left ventricular angiography yielding important information concerning the functional behaviour of the myocardium. In judging the therapeutic value of a.-c. bypass surgery it should be noted that postoperatively 60 to 70 percent of the patients present without symptoms and 80 to 95 percent feel markedly better, and that physical performance is enhanced in about the same proportions. An improvement in left ventricular function under exercise conditions seems to be rare. Hospital mortality of a.-c. bypass operation is small and below 5 percent if patients with stable angina and without myocardial failure, previous infarctions or mitral regurgitation are considered. In the presence of an ischemic cardiomyopathy, on the other hand, the mere surgical risk soon reaches prohibitive limits. The incidence of early complicating myocardial infarctions ranges around 10 percent. Bypass occlusion occurs in some 5 to 15 percent during the early postoperative phase, while in the following months and years the patency rate diminishes but little. If the survival rates of operated and non-operated patients with IHD are compared it becomes evident that a prolongation of life is possible whenever surgery aims at a correction of two- and three-vessel disease (including the prognostically unfavourable isolated stenosis of the left anterior descending branch and stenosis of left main artery) whereas the natural course of isolated lesions of the right coronary artery and the left circumflex branch seems to balance the effect of corresponding surgical interventions. It should be borne in mind, however, that the follow-up periods on which these statements are based do not exceed 3-4 years.", "contents": "[Problems of aortocoronary bypass. Indications for coronary angiography and ventriculography; results of direct bypassing coronary surgery]. The introduction of selective coronary arteriography and aorto-to-coronary saphenous vein bypass surgery (a.-c bypass) has fundamentally altered our understanding of ischemic heart disease (IHD). The indications for the effective diagnostic procedure and the results of the new and increasingly important surgical technique are summarized. Selective coronary arteriography should be performed (a) in patients with known IHD in order to furnish the anatomical and functional information necessary to assess the indication for surgery, i.e. in patients below 60 years with intractable stable or unstable (impending infarction) angina. It is rarely indicated in patients with an old myocardial infarction who are free from symptoms. It is debatable in patients during the acute stage of infarction; (b) in patients with questionable IHD, with the aim of ruling out or confirming the condition, i.e. mainly in patients with atypical chest pain or with equivocal ecg findings. The risks of the procedure, if carried out by experienced personnel, are small. Selective arteriography will always be supplemented by a selective left ventricular angiography yielding important information concerning the functional behaviour of the myocardium. In judging the therapeutic value of a.-c. bypass surgery it should be noted that postoperatively 60 to 70 percent of the patients present without symptoms and 80 to 95 percent feel markedly better, and that physical performance is enhanced in about the same proportions. An improvement in left ventricular function under exercise conditions seems to be rare. Hospital mortality of a.-c. bypass operation is small and below 5 percent if patients with stable angina and without myocardial failure, previous infarctions or mitral regurgitation are considered. In the presence of an ischemic cardiomyopathy, on the other hand, the mere surgical risk soon reaches prohibitive limits. The incidence of early complicating myocardial infarctions ranges around 10 percent. Bypass occlusion occurs in some 5 to 15 percent during the early postoperative phase, while in the following months and years the patency rate diminishes but little. If the survival rates of operated and non-operated patients with IHD are compared it becomes evident that a prolongation of life is possible whenever surgery aims at a correction of two- and three-vessel disease (including the prognostically unfavourable isolated stenosis of the left anterior descending branch and stenosis of left main artery) whereas the natural course of isolated lesions of the right coronary artery and the left circumflex branch seems to balance the effect of corresponding surgical interventions. It should be borne in mind, however, that the follow-up periods on which these statements are based do not exceed 3-4 years."}
{"id": "PMID:1079092", "title": "[Dyserythropoietic anemai].", "content": "A systematic study of dyserythropoietic conditions has been conducted in the light of present knowledge and the author's experience. Cytomorphologic and cytochemical investigations have been made of the following hemopathies: 1. primary idiopathic dyserythropoietic anemia morphologically similar to types I, II and III but noncongenital and without a positive acidified-serum test; 2. a special form of dyserythropoietic anemia associated with dyshemopoietic genotypical mucopolysaccharidosis, a new ly recognized from of inherited mucopolysaccharide storage; 3. dyserythropoietic anemia in a patient with homozygosity due to increased isolated HbA2, a Cooleg-like anemia characterized by very long survival without transfusion treatment; 4. dyserythropoietic changes prior to any treatment in the bone marrow of cases of promyelocytic, myelomonocytoid and basophilic leukemia. The cytomorphologic and serologic features of this clinical material are discussed. Particular reference is made to a new finding, i.e. phagocytosis of degenerated erythroblasts by Alder's granuloblasts, as a new form of congenital dyserythropoietic anemia. Finally, a further dyserythropoietic condition is pointed out which is related to human fetal erythropoiesis and characterized by a large number of reticuloerythroblast islets and a dyserythropoietic pattern. However, although it appears to point to ineffective erythropoiesis this last condition cannot be considered as pathologic but as a teleologic hemopoietic phenomenon.", "contents": "[Dyserythropoietic anemai]. A systematic study of dyserythropoietic conditions has been conducted in the light of present knowledge and the author's experience. Cytomorphologic and cytochemical investigations have been made of the following hemopathies: 1. primary idiopathic dyserythropoietic anemia morphologically similar to types I, II and III but noncongenital and without a positive acidified-serum test; 2. a special form of dyserythropoietic anemia associated with dyshemopoietic genotypical mucopolysaccharidosis, a new ly recognized from of inherited mucopolysaccharide storage; 3. dyserythropoietic anemia in a patient with homozygosity due to increased isolated HbA2, a Cooleg-like anemia characterized by very long survival without transfusion treatment; 4. dyserythropoietic changes prior to any treatment in the bone marrow of cases of promyelocytic, myelomonocytoid and basophilic leukemia. The cytomorphologic and serologic features of this clinical material are discussed. Particular reference is made to a new finding, i.e. phagocytosis of degenerated erythroblasts by Alder's granuloblasts, as a new form of congenital dyserythropoietic anemia. Finally, a further dyserythropoietic condition is pointed out which is related to human fetal erythropoiesis and characterized by a large number of reticuloerythroblast islets and a dyserythropoietic pattern. However, although it appears to point to ineffective erythropoiesis this last condition cannot be considered as pathologic but as a teleologic hemopoietic phenomenon."}
{"id": "PMID:1079093", "title": "[Vestibular dizziness. Differential diagnosis and therapy].", "content": "Dizziness includes a wide variety of subjective sensations which are not conclusive as to their localization or pathogenesis. Even in the absence of other symptoms an analysis of dizziness, including consideration not only of its occurrence but also of its quality and course, can suggest several possibilities for differential diagnosis. We differentiate between positional vertigo, permanent vertigo and attacks of dizziness, which may be of long or only short (seconds to few minutes) duration. Based on this classification the differential diagnosis of vestibularly-induced dizziness, which ranges from distinct entities of peripheral disease (acute vestibulopathy, M\u00e9ni\u00e8re's disease) to symptomatic forms of peripheral or central pathogenesis, is discussed. The symptomatic medical treatment of vestibular dizziness is based on sedation of the central vestibular system. Translabyrinthine vestibular neurectomy is the treatment of choice for drug resistant M\u00e9ni\u00e8re's disease with a high frequency of attacks.", "contents": "[Vestibular dizziness. Differential diagnosis and therapy]. Dizziness includes a wide variety of subjective sensations which are not conclusive as to their localization or pathogenesis. Even in the absence of other symptoms an analysis of dizziness, including consideration not only of its occurrence but also of its quality and course, can suggest several possibilities for differential diagnosis. We differentiate between positional vertigo, permanent vertigo and attacks of dizziness, which may be of long or only short (seconds to few minutes) duration. Based on this classification the differential diagnosis of vestibularly-induced dizziness, which ranges from distinct entities of peripheral disease (acute vestibulopathy, M\u00e9ni\u00e8re's disease) to symptomatic forms of peripheral or central pathogenesis, is discussed. The symptomatic medical treatment of vestibular dizziness is based on sedation of the central vestibular system. Translabyrinthine vestibular neurectomy is the treatment of choice for drug resistant M\u00e9ni\u00e8re's disease with a high frequency of attacks."}
{"id": "PMID:1079095", "title": "Human lymphocytes:  similarity of B and T cell surface morphology.", "content": "When viewed by scanning electron microscopy human lymphocytes fixed in suspension and processed with minimal cell loss appear uniformly covered with short microvilli. Contrary to previous reports, lymphocytes from subpopulations selectively enriched for T cells are villous and indistinguishable from B lymphocytes. Whereas lymphocyte surface architecture can change rapidly and substantially in response to environmental modifications, such as contact with an underlying surface, these alterations are similar for both B and T cells and do not serve to distinguish these subpopulations.", "contents": "Human lymphocytes:  similarity of B and T cell surface morphology. When viewed by scanning electron microscopy human lymphocytes fixed in suspension and processed with minimal cell loss appear uniformly covered with short microvilli. Contrary to previous reports, lymphocytes from subpopulations selectively enriched for T cells are villous and indistinguishable from B lymphocytes. Whereas lymphocyte surface architecture can change rapidly and substantially in response to environmental modifications, such as contact with an underlying surface, these alterations are similar for both B and T cells and do not serve to distinguish these subpopulations."}
{"id": "PMID:1079096", "title": "Intrahepatic cholangioenteric anastomosis in carcinoma of the hilus of the liver.", "content": "It is important to expand the indications for resection of tumors of the hilas, generally requiring associated hepatectomy, after careful search for metastases and biopsy of any suspicious areas makes this reasonable. One can justify such a procedure, representing a major stress and a considerable mortality rate, only if one is sure that all the tumor will be removed. If resection cannot be carried out, a unilateral intrahepatic cholangioenteric anastomosis with preference for the round ligament technique is an excellent procedure when properly applied. If a contraindication to a left sided anastomosis exists, particularly invasion of the left sided confluences, the anastomosis is made on the right to the duct of segment V. Study of the cholangiogram with attenion to the primary and secondary confluences directs a decision to perform a double anastomosis the ducts are not dilated or a poor quality anastomosis is all that can be achieved on one side and when secondary confluents are involved on both sides. The results of a variety of techniques emphasize the importance of the cholangiogram in choosing the location of an anastomosis and the role of invasion of primary and secondary conversions in choosing the technique. Many of thse patients are young and, even if no resection is possible, amy survive several years. A renewed sense of well being and prolongation of life are achievable goals even if the tumor cannot be removed. There is every reason to offer the maximum to these patients so that, if they muse eventually die, it will be from the tumor itself and not from its biliary complications.", "contents": "Intrahepatic cholangioenteric anastomosis in carcinoma of the hilus of the liver. It is important to expand the indications for resection of tumors of the hilas, generally requiring associated hepatectomy, after careful search for metastases and biopsy of any suspicious areas makes this reasonable. One can justify such a procedure, representing a major stress and a considerable mortality rate, only if one is sure that all the tumor will be removed. If resection cannot be carried out, a unilateral intrahepatic cholangioenteric anastomosis with preference for the round ligament technique is an excellent procedure when properly applied. If a contraindication to a left sided anastomosis exists, particularly invasion of the left sided confluences, the anastomosis is made on the right to the duct of segment V. Study of the cholangiogram with attenion to the primary and secondary confluences directs a decision to perform a double anastomosis the ducts are not dilated or a poor quality anastomosis is all that can be achieved on one side and when secondary confluents are involved on both sides. The results of a variety of techniques emphasize the importance of the cholangiogram in choosing the location of an anastomosis and the role of invasion of primary and secondary conversions in choosing the technique. Many of thse patients are young and, even if no resection is possible, amy survive several years. A renewed sense of well being and prolongation of life are achievable goals even if the tumor cannot be removed. There is every reason to offer the maximum to these patients so that, if they muse eventually die, it will be from the tumor itself and not from its biliary complications."}
{"id": "PMID:1079102", "title": "Incidence and prevention of pulmonary embolism after coronary artery surgery.", "content": "Pulmonary embolism is one of the common complications of aortocoronary artery graft surgery. During one year period, 22 patients had documented evidence of pulmonary embolism out of 231 patients who were operated. There were four deaths and all confirmed by autopsy. Clinical signs of phlebitis were absent in many cases of pulmonary embolism. Elastic stockings were not effective to prevent phlebitis. Heparin did not prevent deaths in all four patients. Heparin therapy and aggressive caval interruption by Modin-Uddin umbrella has eliminated deaths in the last 450 cases.", "contents": "Incidence and prevention of pulmonary embolism after coronary artery surgery. Pulmonary embolism is one of the common complications of aortocoronary artery graft surgery. During one year period, 22 patients had documented evidence of pulmonary embolism out of 231 patients who were operated. There were four deaths and all confirmed by autopsy. Clinical signs of phlebitis were absent in many cases of pulmonary embolism. Elastic stockings were not effective to prevent phlebitis. Heparin did not prevent deaths in all four patients. Heparin therapy and aggressive caval interruption by Modin-Uddin umbrella has eliminated deaths in the last 450 cases."}
{"id": "PMID:1079105", "title": "[Obtaining specific non-adsorbed sera to Haemophilus influenza for the agglutination test on glass].", "content": "Type-specific agglutinating sera to capsular antigenas of H. influenzae were recovered (from 1:3200 to 1:25600). With the given immunization scheme there formed mostly IgG immunoglobulins, and only insignificant amount of IgM (separation of G-200 Sephadex gel). IgG were found to be responsible for the type-specificity of the sera to bacteria, whereas the cross reactions within the H. influenzae genus were connected connected with the IgM. Use of the sera heated at various temperature regimens offered a possibility of obtaining highly specific agglutinating sera with the aid of which express serodiagnosis of the isolated H. influenzae strains was possible.", "contents": "[Obtaining specific non-adsorbed sera to Haemophilus influenza for the agglutination test on glass]. Type-specific agglutinating sera to capsular antigenas of H. influenzae were recovered (from 1:3200 to 1:25600). With the given immunization scheme there formed mostly IgG immunoglobulins, and only insignificant amount of IgM (separation of G-200 Sephadex gel). IgG were found to be responsible for the type-specificity of the sera to bacteria, whereas the cross reactions within the H. influenzae genus were connected connected with the IgM. Use of the sera heated at various temperature regimens offered a possibility of obtaining highly specific agglutinating sera with the aid of which express serodiagnosis of the isolated H. influenzae strains was possible."}
{"id": "PMID:1079107", "title": "Lupus-like syndrome associated with pulmonary reaction to nitrofurantoin. Report of three cases.", "content": "A systemic lupus erythematosus-like syndrome developed simultaneously with pulmonary reactions of the chronic type in three female patients after treatment with nitrofurantoin for 12, 27 and 38 months, respectively. The syndrome was characterized by elevated ESR, polyclonal hypergammaglobulinaemia, the presence of IgG antinuclear antibodies and a positive latex-fixation test. Two patients had severe arthralgia and one of them peripheral lymphadenopathy. Pleural effusion and a chronic active hepatitis were present in the third patient, in whom interstitial cystitis also developed. All signs and symptoms of the lupus-like syndrome disappeared, without corticosteroid or other medication, when nitrofurantoin was omitted. The diminution of pulmonary infiltrates and the reversal of interstitial cystitis also appeared to be directly related to cessation of nitrofurantoin therapy. Our findings indicate that long-term medication with nitrofurantoin may cause, in addition to pulmonary changes, a simultaneous lupus-like syndrome our data also raise the possibility that interstitial cystitis may occur as an adverse reaction to nitrofurantoin therapy.", "contents": "Lupus-like syndrome associated with pulmonary reaction to nitrofurantoin. Report of three cases. A systemic lupus erythematosus-like syndrome developed simultaneously with pulmonary reactions of the chronic type in three female patients after treatment with nitrofurantoin for 12, 27 and 38 months, respectively. The syndrome was characterized by elevated ESR, polyclonal hypergammaglobulinaemia, the presence of IgG antinuclear antibodies and a positive latex-fixation test. Two patients had severe arthralgia and one of them peripheral lymphadenopathy. Pleural effusion and a chronic active hepatitis were present in the third patient, in whom interstitial cystitis also developed. All signs and symptoms of the lupus-like syndrome disappeared, without corticosteroid or other medication, when nitrofurantoin was omitted. The diminution of pulmonary infiltrates and the reversal of interstitial cystitis also appeared to be directly related to cessation of nitrofurantoin therapy. Our findings indicate that long-term medication with nitrofurantoin may cause, in addition to pulmonary changes, a simultaneous lupus-like syndrome our data also raise the possibility that interstitial cystitis may occur as an adverse reaction to nitrofurantoin therapy."}
{"id": "PMID:1079110", "title": "Hypernatremia following gastrointestinal bleeding in cirrhosis with ascites.", "content": "3 patients with hepatic cirrhosis and ascites underwent increased diuresis on six occasions, closely related to episodes of gastrointestinal bleeding. In each instance the increased urine volume was preceded by a sharp increase in blood urea nitrogen, presumably due to absorption of nitrogenous compounds from the gastrointestinal tract, suggesting a mechanism of osmotic diuresis. In each case there was a signigicant increase in serum sodium and osmolality, related to the greater-water-than-sodium diuresis induced by urea, which was promptly reversed by the administration of water or isotonic solution. Clinically this syndrome may be defined as the association of hypernatremia and hyperosmolality due to osmotic diuresis from urea appearing in a cirrhotic patient with ascites and gastrointestinal bleeding.", "contents": "Hypernatremia following gastrointestinal bleeding in cirrhosis with ascites. 3 patients with hepatic cirrhosis and ascites underwent increased diuresis on six occasions, closely related to episodes of gastrointestinal bleeding. In each instance the increased urine volume was preceded by a sharp increase in blood urea nitrogen, presumably due to absorption of nitrogenous compounds from the gastrointestinal tract, suggesting a mechanism of osmotic diuresis. In each case there was a signigicant increase in serum sodium and osmolality, related to the greater-water-than-sodium diuresis induced by urea, which was promptly reversed by the administration of water or isotonic solution. Clinically this syndrome may be defined as the association of hypernatremia and hyperosmolality due to osmotic diuresis from urea appearing in a cirrhotic patient with ascites and gastrointestinal bleeding."}
{"id": "PMID:1079111", "title": "Alcohol injury to gastric mucosa in mice and its potentiation by stress.", "content": "This investigation was initiated to separately evaluate the roles of dose and concentration of ethanol ingestion in the development of acute gastric mucosal injury and to determine the significance of stress as a potentiating factor in ethanol damage. A total of 423 mice were used in this study. Alcohol at the low concentration of 10% and at any of the doses used (1-5 g/kh wt) did not cause gastric mucosal lesions. Similarly, alcohol at the low dose of 1 g/kg wt at any of the concentrations used (10-50%) did not produce any gross injury to the stomach. A single oral administration of ethanol, given at doses of 2-5 g/kg wt and at concentrations of 25-50%, resulted in hyperemia and multiple fundic erosions and, less frequently, antral erosion. The incidence, number, and severity of these lesions rapidly increased with increase in ethanol concentration, ethanol dose, or both. Healing was rapid; at best, lesions were only barely visible 72 hours after alcohol ingestion. Repeated daily doses of ethanol given for 3-5 days did not increase the incidence and number of lesions, probably due to their capacity for rapid healing. Mild stress, in the form of 1-hour restraint in the cold room at 8 degrees C, by itself caused very little mucosal injury in mice, but significantly potentiated the injurious effect of 35% ethanol administered in a 3 g/kh dose. Stress following alcohol intake potentiated gastric mucosal injury much more than if the stress preceded the alcohol ingestion. The incidence, number, and severity of erosions was here 3,4 and 11 times, respectively, greater than the additive effect of alcohol and stress together (P smaller than 0.001). Thus, the extent of ethanol damage to the stomach related both to the concentration and dose of ethanol infested. Stress, especially when following excessive ethanol intake, was a highly significant factor in the potentiation of acute alcoholic gastric mucosal injury.", "contents": "Alcohol injury to gastric mucosa in mice and its potentiation by stress. This investigation was initiated to separately evaluate the roles of dose and concentration of ethanol ingestion in the development of acute gastric mucosal injury and to determine the significance of stress as a potentiating factor in ethanol damage. A total of 423 mice were used in this study. Alcohol at the low concentration of 10% and at any of the doses used (1-5 g/kh wt) did not cause gastric mucosal lesions. Similarly, alcohol at the low dose of 1 g/kg wt at any of the concentrations used (10-50%) did not produce any gross injury to the stomach. A single oral administration of ethanol, given at doses of 2-5 g/kg wt and at concentrations of 25-50%, resulted in hyperemia and multiple fundic erosions and, less frequently, antral erosion. The incidence, number, and severity of these lesions rapidly increased with increase in ethanol concentration, ethanol dose, or both. Healing was rapid; at best, lesions were only barely visible 72 hours after alcohol ingestion. Repeated daily doses of ethanol given for 3-5 days did not increase the incidence and number of lesions, probably due to their capacity for rapid healing. Mild stress, in the form of 1-hour restraint in the cold room at 8 degrees C, by itself caused very little mucosal injury in mice, but significantly potentiated the injurious effect of 35% ethanol administered in a 3 g/kh dose. Stress following alcohol intake potentiated gastric mucosal injury much more than if the stress preceded the alcohol ingestion. The incidence, number, and severity of erosions was here 3,4 and 11 times, respectively, greater than the additive effect of alcohol and stress together (P smaller than 0.001). Thus, the extent of ethanol damage to the stomach related both to the concentration and dose of ethanol infested. Stress, especially when following excessive ethanol intake, was a highly significant factor in the potentiation of acute alcoholic gastric mucosal injury."}
{"id": "PMID:1079112", "title": "The effect of neuraminidase on genetic variants of alpha anitrypsin.", "content": "Sera of Pi types M, F, S, Z, IM, FM, MS, and MZ were incubated with neuraminidase and the reaction products followed by electrophoresis. The alpha1 antitrypsin components showed a series of changes in mobility as sialic residues were removed. Removal of sialic acid was confirmed by chemical assay. Results of studies with two different electrophoretic systems suggested that the Z type alpha1 antitrypsin has less sialic acid than the M, F, and S types. There was no evidence that other genetic variants have a reduced sialic acid content. The two major bands of alpha1 antitrypsin seen in certain electrophoretic systems may reflect a difference of one sialic acid residue. It is proposed that the Z protein lacks a carbohydrate chain with two terminal sialic acid residues. This carbohydrate deficiency results in lack of secretion of type Z alpha1 antitrypsin from the endoplasmic reticulum, perhaps because of binding to sites specific for the incomplete glycoprotein or because of aggregation of the Z asialo protein. A carbohydrate chain could be prevented from attaching to the Z type either because of a conformational change or because of the replacement of a carbohydrate-binding asparagine residue in the Z protein.", "contents": "The effect of neuraminidase on genetic variants of alpha anitrypsin. Sera of Pi types M, F, S, Z, IM, FM, MS, and MZ were incubated with neuraminidase and the reaction products followed by electrophoresis. The alpha1 antitrypsin components showed a series of changes in mobility as sialic residues were removed. Removal of sialic acid was confirmed by chemical assay. Results of studies with two different electrophoretic systems suggested that the Z type alpha1 antitrypsin has less sialic acid than the M, F, and S types. There was no evidence that other genetic variants have a reduced sialic acid content. The two major bands of alpha1 antitrypsin seen in certain electrophoretic systems may reflect a difference of one sialic acid residue. It is proposed that the Z protein lacks a carbohydrate chain with two terminal sialic acid residues. This carbohydrate deficiency results in lack of secretion of type Z alpha1 antitrypsin from the endoplasmic reticulum, perhaps because of binding to sites specific for the incomplete glycoprotein or because of aggregation of the Z asialo protein. A carbohydrate chain could be prevented from attaching to the Z type either because of a conformational change or because of the replacement of a carbohydrate-binding asparagine residue in the Z protein."}
{"id": "PMID:1079113", "title": "Mass screening of newborn Swedish infants for alpha antitrypsin deficiency.", "content": "During the first year of mass screening for alpha1 antitrypsin deficiency, 108,000 newborn Swedish infants were examined. The screening method and criteria used for selecting infants with alpha1 antitrypsin deficiency were reliable. The Pi Z phenotype occurred at a rate of one per 1,433 infants, and the PiZ allele frequency was .026. The Pi- allele was found in one Pi Z and one Pi S infant. The Pi- allele frequency probably was below .001.", "contents": "Mass screening of newborn Swedish infants for alpha antitrypsin deficiency. During the first year of mass screening for alpha1 antitrypsin deficiency, 108,000 newborn Swedish infants were examined. The screening method and criteria used for selecting infants with alpha1 antitrypsin deficiency were reliable. The Pi Z phenotype occurred at a rate of one per 1,433 infants, and the PiZ allele frequency was .026. The Pi- allele was found in one Pi Z and one Pi S infant. The Pi- allele frequency probably was below .001."}
{"id": "PMID:1079114", "title": "Improved long-term survival after aortocoronary bypass for advanced coronary artery disease.", "content": "Three hundred thirty patients undergoing coronary bypass grafts for disabling angina, \"preinfarction\" angina, or coronary occlusion without cardiogenic shock have undergone coronary revascularization from July 1970 to March 1974. The operative mortality was 1.2 per cent and the long-term mortality, 4 per cent. Patients were subjected to life table analysis, and the figures suggest that in patients with two- and three-vessel coronary artery disease who received complete revascularization, there was a significant prolongation of life when compared with data from a large series of medically treated patients with angiographically documented coronary artery disease. Longer follow-up data will be important in definitively ascertaining the favorable effect of coronary revascularization on longevity.", "contents": "Improved long-term survival after aortocoronary bypass for advanced coronary artery disease. Three hundred thirty patients undergoing coronary bypass grafts for disabling angina, \"preinfarction\" angina, or coronary occlusion without cardiogenic shock have undergone coronary revascularization from July 1970 to March 1974. The operative mortality was 1.2 per cent and the long-term mortality, 4 per cent. Patients were subjected to life table analysis, and the figures suggest that in patients with two- and three-vessel coronary artery disease who received complete revascularization, there was a significant prolongation of life when compared with data from a large series of medically treated patients with angiographically documented coronary artery disease. Longer follow-up data will be important in definitively ascertaining the favorable effect of coronary revascularization on longevity."}
{"id": "PMID:1079116", "title": "Ultrastructural variation in granular dystrophy of the cornea.", "content": "Corneal deposits of granular dystrophy were studies in six corneas from five patients. Two additional corneas from two patients with lattice dystrophy were used for comparison. The diagnoses were confirmed by light and electron microscopy. Three types of ultrastructural features were distinguished in the electron-dense rod-shaped deposits of granular dystrophy, i.e., Type 1 with homogenous structure, Type 2 with fibrillar constituents, and Type 3 with a moth-eaten or sponge-like appearance. In polarization microscopy of Congo-red stained sections, the Type 2 tended to show an increased birefringence without green dichroism, while the other types were less birefringent than the stroma. Three different components were found immediately around the deposits, i.e., 1. normal collagen fibrils, 2. finely filamentous substance, and 3. areas of thin fibrils (70-100 A in diameter). While the second component appeared non-specific, the third component was found only in two corneas, superimposed on the lesions of granular dystrophy, and showed light and electron microscopic characteristics of the lesions of amyloid as well as of lattice dystrophy.", "contents": "Ultrastructural variation in granular dystrophy of the cornea. Corneal deposits of granular dystrophy were studies in six corneas from five patients. Two additional corneas from two patients with lattice dystrophy were used for comparison. The diagnoses were confirmed by light and electron microscopy. Three types of ultrastructural features were distinguished in the electron-dense rod-shaped deposits of granular dystrophy, i.e., Type 1 with homogenous structure, Type 2 with fibrillar constituents, and Type 3 with a moth-eaten or sponge-like appearance. In polarization microscopy of Congo-red stained sections, the Type 2 tended to show an increased birefringence without green dichroism, while the other types were less birefringent than the stroma. Three different components were found immediately around the deposits, i.e., 1. normal collagen fibrils, 2. finely filamentous substance, and 3. areas of thin fibrils (70-100 A in diameter). While the second component appeared non-specific, the third component was found only in two corneas, superimposed on the lesions of granular dystrophy, and showed light and electron microscopic characteristics of the lesions of amyloid as well as of lattice dystrophy."}
{"id": "PMID:1079117", "title": "Goldmann applanation versus hand-applanation and .schi\u00f6tz indentation tonometry.", "content": "For evaluation of hand-applanation tonometry comparative measurements of intraocular pressure have been carried out using the Goldmann applanation tonometer, the Draeger and Perkins hand-applanation tonometers and the Schi\u00f6tz indentation tonometer. A series of 52 eyes has been tested. The statistical analysis was based on the Goldmann tonometry as a reference system. The hand-applanation tonometers were found to be in satisfactory agreement with the Goldmann tonometer, whereas the Schi\u00f6tz tonometer gave significantly low values in the pressure range considered.", "contents": "Goldmann applanation versus hand-applanation and .schi\u00f6tz indentation tonometry. For evaluation of hand-applanation tonometry comparative measurements of intraocular pressure have been carried out using the Goldmann applanation tonometer, the Draeger and Perkins hand-applanation tonometers and the Schi\u00f6tz indentation tonometer. A series of 52 eyes has been tested. The statistical analysis was based on the Goldmann tonometry as a reference system. The hand-applanation tonometers were found to be in satisfactory agreement with the Goldmann tonometer, whereas the Schi\u00f6tz tonometer gave significantly low values in the pressure range considered."}
{"id": "PMID:1079118", "title": "[Intraocular pressure after changing the patient's position (author's transl)].", "content": "The influence of the patient's position (sitting or supine) on the intraocular pressure has been examined by means of Goldmann applanation-tonometry. The statistical analysis shows that the intraocular pressure decreases after the first measurement. This is the reason for measuring higher values in the first position, irrespective wether the first measurement is done in sitting or supine position. Analysing the values of steady state pressure i.e. 12 min after taking or changing position indicates a little higher mean value of intraocular pressure in the sitting position of the patient. The difference of mean valuse of sitting ans supine position of the patient is not significant. There are patients who have always higher others have always lower values of intraocular pressure in the supine position. These observations described earlier by Strobl and Follmann, have been confirmed by repeated measurements on 19 patients with differences in both directions of 2 to 4 mm Hg. For this reason it seems not feasible to use a statistical mean value of a population to correct the intraocular pressure of a particular case after changing position.", "contents": "[Intraocular pressure after changing the patient's position (author's transl)]. The influence of the patient's position (sitting or supine) on the intraocular pressure has been examined by means of Goldmann applanation-tonometry. The statistical analysis shows that the intraocular pressure decreases after the first measurement. This is the reason for measuring higher values in the first position, irrespective wether the first measurement is done in sitting or supine position. Analysing the values of steady state pressure i.e. 12 min after taking or changing position indicates a little higher mean value of intraocular pressure in the sitting position of the patient. The difference of mean valuse of sitting ans supine position of the patient is not significant. There are patients who have always higher others have always lower values of intraocular pressure in the supine position. These observations described earlier by Strobl and Follmann, have been confirmed by repeated measurements on 19 patients with differences in both directions of 2 to 4 mm Hg. For this reason it seems not feasible to use a statistical mean value of a population to correct the intraocular pressure of a particular case after changing position."}
{"id": "PMID:1079119", "title": "[Identification of zonula fiber protein by disc-electrophoresis (author's transl)].", "content": "By our investigations we found identity of collagen and zonula fibers protein. In Disk-electrophoresis both the zonula fibers ans the fibers of the vitreous show the alpha-component only. Therefore it is likely that these proteins are closely related.", "contents": "[Identification of zonula fiber protein by disc-electrophoresis (author's transl)]. By our investigations we found identity of collagen and zonula fibers protein. In Disk-electrophoresis both the zonula fibers ans the fibers of the vitreous show the alpha-component only. Therefore it is likely that these proteins are closely related."}
{"id": "PMID:1079120", "title": "[Determination of bovine aqueous humor proteins (author's transl)].", "content": "By turbidimetry, Disk-electrophoresis, combined Disk-immunomethod, and single radial immunodiffusion it was possible both to identify some proteins and to determine the protein content and the albumine concentration of bovine aqueous humor.", "contents": "[Determination of bovine aqueous humor proteins (author's transl)]. By turbidimetry, Disk-electrophoresis, combined Disk-immunomethod, and single radial immunodiffusion it was possible both to identify some proteins and to determine the protein content and the albumine concentration of bovine aqueous humor."}
{"id": "PMID:1079121", "title": "Alkali and acid burns of the rabbit eye: uptake of intravenously injected Na125I and Na131I into the cornea and iris.", "content": "Rabbit eyes were burned either with 1 N sodium hydroxide or 1 N hydrochloric acid. In a previous part of the study (Obenberger and Babick\u00fd, 1974) authors measured the aqueous flow using intravenous injections of Na125I and Na 131I. In the present study the uptake of iodine into the cornea and iris is investigated in the same eyes. Caustic injuries of both types initially cause changes in the specific activity of the aqueous affecting the uptake of iodine into the examined tissues.", "contents": "Alkali and acid burns of the rabbit eye: uptake of intravenously injected Na125I and Na131I into the cornea and iris. Rabbit eyes were burned either with 1 N sodium hydroxide or 1 N hydrochloric acid. In a previous part of the study (Obenberger and Babick\u00fd, 1974) authors measured the aqueous flow using intravenous injections of Na125I and Na 131I. In the present study the uptake of iodine into the cornea and iris is investigated in the same eyes. Caustic injuries of both types initially cause changes in the specific activity of the aqueous affecting the uptake of iodine into the examined tissues."}
{"id": "PMID:1079124", "title": "Immunologic studies in patients with recurrent bronchopulmonary infections.", "content": "In view of the known association of several different immune deficiency disorders with infections of the respiratory tract, a number of immunologic parameters were investigated in 15 patients with recurrent brochopulmonary infections. The patients' neutrophils all demonstrated normal intracellular killing of Staphylococcus aureus, and responded normally to chemotactic stimuli. Although assays of complement 3c were normal in all patients' sera, defective total hemolytic complement activity was consistently shown in 1 patinets manifested elevated serum immunoglobulin concentrations. Severe alpha1 -antitrypsin deficiency (Pi ZZ phenotype) was discovered in 1 patient. Three patients displayed cutaneous anergy to a battery of skin-test antigens, but lymphocytes from all patients studied responded normally to in vitro phytohemagglutinin stimulation. A \"blocking\" factor found in the serum of 3 patients suppressed the in bitro transformation of normal Candida-responsive lymphocytes by Candida albicans antigen. \"Cold\" lymphocytotoxins were detected in the sera of 10 patients, but in only 3 of 19 control sera. Although the significance of these findings is unknown, they may possibly have pathogenetic relevance in a small number of the patients studied.", "contents": "Immunologic studies in patients with recurrent bronchopulmonary infections. In view of the known association of several different immune deficiency disorders with infections of the respiratory tract, a number of immunologic parameters were investigated in 15 patients with recurrent brochopulmonary infections. The patients' neutrophils all demonstrated normal intracellular killing of Staphylococcus aureus, and responded normally to chemotactic stimuli. Although assays of complement 3c were normal in all patients' sera, defective total hemolytic complement activity was consistently shown in 1 patinets manifested elevated serum immunoglobulin concentrations. Severe alpha1 -antitrypsin deficiency (Pi ZZ phenotype) was discovered in 1 patient. Three patients displayed cutaneous anergy to a battery of skin-test antigens, but lymphocytes from all patients studied responded normally to in vitro phytohemagglutinin stimulation. A \"blocking\" factor found in the serum of 3 patients suppressed the in bitro transformation of normal Candida-responsive lymphocytes by Candida albicans antigen. \"Cold\" lymphocytotoxins were detected in the sera of 10 patients, but in only 3 of 19 control sera. Although the significance of these findings is unknown, they may possibly have pathogenetic relevance in a small number of the patients studied."}
{"id": "PMID:1079126", "title": "Transvestibular approach to the internal auditory canal.", "content": "Summary--A surgical approach to the internal auditory canal is presented which as proved useful in the following circumstances: 1) as a destructive procedure in M\u00e9ni\u00e8re's disease, 2) as a diagnostic procedure in patients in whom an acoustic neurinoma is suspected and a labrinthectomy is indicated. In this situation the tumor may be removed during the same operative procedure, 3) for the removal of small, mobile, intracanalicular neurionomas, and 4) for complete facial nerve decompression in patients with no vestibular response and no serviceable hearing.", "contents": "Transvestibular approach to the internal auditory canal. Summary--A surgical approach to the internal auditory canal is presented which as proved useful in the following circumstances: 1) as a destructive procedure in M\u00e9ni\u00e8re's disease, 2) as a diagnostic procedure in patients in whom an acoustic neurinoma is suspected and a labrinthectomy is indicated. In this situation the tumor may be removed during the same operative procedure, 3) for the removal of small, mobile, intracanalicular neurionomas, and 4) for complete facial nerve decompression in patients with no vestibular response and no serviceable hearing."}
{"id": "PMID:1079129", "title": "Studies with Brugia pahangi. III: Histological changes in the affected lymph nodes of infected cats.", "content": "Cats infected with Brugia pahangi by single or repeated inoculation of infective larvae for different periods of time, were autopsied and the affected lymph nodes examined histologically, stained with methyl green and pyronin Y, haematoxylin and eosin, Lendrum's eosinophil stain, Mallory's trichrome stain or the May-Grunwald Giemsa technique. In single infections, there was initially a cell-mediated type immunological response which was characterized by proliferation of mononuclear thymus-dependent cells in the paracortical region of the lymph node. The antibody-type response became more evident as the infections progressed. This response was characterized by proliferation of lymphocytes in rapidly enlarging germinal centres and the production of large numbers of plasma cells in the cortico-medullary junction and medullary cords of the nodes. In long term infections, the affected afferent lymphatic vessel and popliteal node were almost obliterated by fibrous tissue, but an inguinal lymph node complex usually developed to replace lymphatic filtration in the limb. Repeated infections produced more complicated changes. Both types of response occurred to varying degrees throughout the infection. No significant increase in mast cells or eosinophil cells was seen. Fibrous tissue increased in the lymph nodes only after long periods of infection; such nodes often became non-functional, and by-passed by new lymphatic vessels.", "contents": "Studies with Brugia pahangi. III: Histological changes in the affected lymph nodes of infected cats. Cats infected with Brugia pahangi by single or repeated inoculation of infective larvae for different periods of time, were autopsied and the affected lymph nodes examined histologically, stained with methyl green and pyronin Y, haematoxylin and eosin, Lendrum's eosinophil stain, Mallory's trichrome stain or the May-Grunwald Giemsa technique. In single infections, there was initially a cell-mediated type immunological response which was characterized by proliferation of mononuclear thymus-dependent cells in the paracortical region of the lymph node. The antibody-type response became more evident as the infections progressed. This response was characterized by proliferation of lymphocytes in rapidly enlarging germinal centres and the production of large numbers of plasma cells in the cortico-medullary junction and medullary cords of the nodes. In long term infections, the affected afferent lymphatic vessel and popliteal node were almost obliterated by fibrous tissue, but an inguinal lymph node complex usually developed to replace lymphatic filtration in the limb. Repeated infections produced more complicated changes. Both types of response occurred to varying degrees throughout the infection. No significant increase in mast cells or eosinophil cells was seen. Fibrous tissue increased in the lymph nodes only after long periods of infection; such nodes often became non-functional, and by-passed by new lymphatic vessels."}
{"id": "PMID:1079130", "title": "Simplified circular aortotomy in aortocoronary bypass operations.", "content": "Adaptation of a rongeur technique facilitates aortotomy in aortocoronary bypass operations. A single motion with a double-action duckbilled rongeur produces a smooth-walled, symmetrical aortotomy which varies in shape predictably according to the angle at which the rongeur is held relative to the axis of the aorta.", "contents": "Simplified circular aortotomy in aortocoronary bypass operations. Adaptation of a rongeur technique facilitates aortotomy in aortocoronary bypass operations. A single motion with a double-action duckbilled rongeur produces a smooth-walled, symmetrical aortotomy which varies in shape predictably according to the angle at which the rongeur is held relative to the axis of the aorta."}
{"id": "PMID:1079131", "title": "Sarcoplasmic masses. Enzyme histochemistry and autoradiography.", "content": "A comparative enzyme-histochemical study was made of the sarcoplasmic masses in a muscle biopsy specimen from a patient with myotonic dystrophy and those in the jaw-muscle of the Rana temporaria tadpole. The enzyme-histochemical patterns of the two methods proved to be identical. An autoradiographic study was made of the sarcoplasmic masses in the R temporaria tadpole with DL-thyroxine-2-14C and levomethionine 35S. The radioactivity labeled compounds were found to be stored or incorporated mainly in the myofibrillae, and virtually not at all in the sarcoplasmic masses.", "contents": "Sarcoplasmic masses. Enzyme histochemistry and autoradiography. A comparative enzyme-histochemical study was made of the sarcoplasmic masses in a muscle biopsy specimen from a patient with myotonic dystrophy and those in the jaw-muscle of the Rana temporaria tadpole. The enzyme-histochemical patterns of the two methods proved to be identical. An autoradiographic study was made of the sarcoplasmic masses in the R temporaria tadpole with DL-thyroxine-2-14C and levomethionine 35S. The radioactivity labeled compounds were found to be stored or incorporated mainly in the myofibrillae, and virtually not at all in the sarcoplasmic masses."}
{"id": "PMID:1079132", "title": "[Histogenesis of the immune system of the \"nude\" mouse. Postnatal development of the thymus: a light microscopical study (author's transl)].", "content": "Mice homozygous for the mutation \"nude\" display a dysgenetic thymus (Pantelouris, 1968). The anterior mediastinum of these animals contains a rudimentary epithelial organ (pantelouris and Hair, 1970), which has been assumed to represent not a maldeveloped thymus, but rather a remnant of the parathyroid gland or of the ultimobranchial body (Wortis et al., 1971). We have investigated the development of the thymus in homo- and heterozygous nude by both light and electron microscopy. This paper describes the light microscopical findings in embryos and fetuses from the 11th up to the 18th day post conceptionem (pc). Homo- and heterozygous nude (nu/nu + nu/+) mice with the genetic background BALB/c were kept under specific pathogen-free (spf) conditions. Virgin nu/+ females were mated with nu/nu males. In addition, homozygous females were mated with homozygous males in order to investigate 11-day-old homozygous embryos. The embryos and fetuses were fixed in Bouin's solution and embedded in paraffin wax. Serial frontal sections (5mu) were stained with hematoxylin and eosin. Up to the 12th day pc, the thymus anlage of the n/nu embryo is indistinguishable from that of the nu/+ animal. The paired anlage develops from the epithelium of the ventral and dorsal diverticles of the 3rd pharyngeal pouch, and from the ectodermal vesicula cervicalis. In the nu/+ embryo, the thymic epithelium begins to proliferate between the 12th day and 13th day pc. In the central part of the anlage, the epithelial cells dissociate and begin to form a reticulum. At the same time, blood vessels from the surrounding mesenchyme invade the anlage. From the 14th day onwards, lymphoblasts are recognizable..", "contents": "[Histogenesis of the immune system of the \"nude\" mouse. Postnatal development of the thymus: a light microscopical study (author's transl)]. Mice homozygous for the mutation \"nude\" display a dysgenetic thymus (Pantelouris, 1968). The anterior mediastinum of these animals contains a rudimentary epithelial organ (pantelouris and Hair, 1970), which has been assumed to represent not a maldeveloped thymus, but rather a remnant of the parathyroid gland or of the ultimobranchial body (Wortis et al., 1971). We have investigated the development of the thymus in homo- and heterozygous nude by both light and electron microscopy. This paper describes the light microscopical findings in embryos and fetuses from the 11th up to the 18th day post conceptionem (pc). Homo- and heterozygous nude (nu/nu + nu/+) mice with the genetic background BALB/c were kept under specific pathogen-free (spf) conditions. Virgin nu/+ females were mated with nu/nu males. In addition, homozygous females were mated with homozygous males in order to investigate 11-day-old homozygous embryos. The embryos and fetuses were fixed in Bouin's solution and embedded in paraffin wax. Serial frontal sections (5mu) were stained with hematoxylin and eosin. Up to the 12th day pc, the thymus anlage of the n/nu embryo is indistinguishable from that of the nu/+ animal. The paired anlage develops from the epithelium of the ventral and dorsal diverticles of the 3rd pharyngeal pouch, and from the ectodermal vesicula cervicalis. In the nu/+ embryo, the thymic epithelium begins to proliferate between the 12th day and 13th day pc. In the central part of the anlage, the epithelial cells dissociate and begin to form a reticulum. At the same time, blood vessels from the surrounding mesenchyme invade the anlage. From the 14th day onwards, lymphoblasts are recognizable.."}
{"id": "PMID:1079134", "title": "Fate of coronary collateral circulation after aorto-coronary saphenous vein bypass grafts.", "content": "The pre- and postoperative patterns of coronary artery collateral circulation have been studied in 34 patients who had saphenous vein bypass grafting. When the graft remained patent homocoronary collaterals could not be visualized after operation, but new intercoronary anastomoses frequently developed to other diseased arteries. When the graft and the bypassed artery were both obstructed there was a high incidence (5 out of 11) of myocardial infarction despite good preoperative collaterals.", "contents": "Fate of coronary collateral circulation after aorto-coronary saphenous vein bypass grafts. The pre- and postoperative patterns of coronary artery collateral circulation have been studied in 34 patients who had saphenous vein bypass grafting. When the graft remained patent homocoronary collaterals could not be visualized after operation, but new intercoronary anastomoses frequently developed to other diseased arteries. When the graft and the bypassed artery were both obstructed there was a high incidence (5 out of 11) of myocardial infarction despite good preoperative collaterals."}
{"id": "PMID:1079135", "title": "Effect of aorto-coronary grafts and native vessel patency on the occurrence of angina pectoris after coronary bypass surgery.", "content": "Exercise testing of 52 patients on average 11 months after coronary bypass surgery for the relief of angina pectoris disclosed improvement in total work (P is less than 0.001), maximal tolerated load (P is smaller than 0.001), maximal heart rate (P is smaller than 0.01), and reduction of maximal ST segment depression (P is smaller than 0.001) in a group of 36 patients with all grafts patent. In another group of 16 patients with one or more grafts occluded the only significant change was a reduction in the maximal ST segment depression (P is smaller than 0.01). Early and late postoperative angiograms showed that 75 per cent of the grafts that became occluded were already closed a few weeks after operation. Occluded grafts were accompanied by persistence of collaterals, which disappeared or dimished in the majority of patients with patent grafts. Progression in native vessel lesions occurred in 40 per cent of patients.  It was related to the grafting procedure (P is smaller than 0.01) but not to the state of grafts. The change in native vessels and other variables studied was equal in the patent and occluded graft groups, justifying the conclusion that graft patency was the major factor alleviating angina after operation.", "contents": "Effect of aorto-coronary grafts and native vessel patency on the occurrence of angina pectoris after coronary bypass surgery. Exercise testing of 52 patients on average 11 months after coronary bypass surgery for the relief of angina pectoris disclosed improvement in total work (P is less than 0.001), maximal tolerated load (P is smaller than 0.001), maximal heart rate (P is smaller than 0.01), and reduction of maximal ST segment depression (P is smaller than 0.001) in a group of 36 patients with all grafts patent. In another group of 16 patients with one or more grafts occluded the only significant change was a reduction in the maximal ST segment depression (P is smaller than 0.01). Early and late postoperative angiograms showed that 75 per cent of the grafts that became occluded were already closed a few weeks after operation. Occluded grafts were accompanied by persistence of collaterals, which disappeared or dimished in the majority of patients with patent grafts. Progression in native vessel lesions occurred in 40 per cent of patients.  It was related to the grafting procedure (P is smaller than 0.01) but not to the state of grafts. The change in native vessels and other variables studied was equal in the patent and occluded graft groups, justifying the conclusion that graft patency was the major factor alleviating angina after operation."}
{"id": "PMID:1079136", "title": "Secondary postpartum haemorrhage.", "content": "The clinical features of 106 women who had a secondary postpartum haemorrhage were examined in detail; 39 were managed conservatively, 3 had a vaginal laceration resutured and 64 had a curettage. In 46 patients, material obtained by curettage was examined histologically. The clinical features associated with retained products of conception are defined and comparisons are made between conservative and surgical management of secondary postpartum haemorrhage.", "contents": "Secondary postpartum haemorrhage. The clinical features of 106 women who had a secondary postpartum haemorrhage were examined in detail; 39 were managed conservatively, 3 had a vaginal laceration resutured and 64 had a curettage. In 46 patients, material obtained by curettage was examined histologically. The clinical features associated with retained products of conception are defined and comparisons are made between conservative and surgical management of secondary postpartum haemorrhage."}
{"id": "PMID:1079137", "title": "Secondary anterior crocodile shagreen of Vogt.", "content": "The clincopathological features and pathogenesis of secondary mosaic degeneration of the cornea (anterior crocodile shagreen of Vogt) are described. The structural basis for the normal anterior corneal mosaic pattern seems to lie in the particular arrangement of many prominent collagen lamellae of the anterior stroma that thake an oblique course to gain insertion into Bowman's layer. Since, at normal intraocular pressure, Bowman's layer is under tension, when viewed from the anterior surface the cornea appears smooth. By releasing the tension, however, a reproducible polygonal ridge pattern becomes manifest. It is suggested that a prolonged phthisical state of the eye is one condition wherein the mosaic pattern may become permanent and that, as a secondary event, this is followed by irregular calcification of Bowman's layer which particularly involves the ridges projecting into the epithelium. Biomicroscopically these ridges corresponded to the branching reticular arrangement of the mosaic opacities.", "contents": "Secondary anterior crocodile shagreen of Vogt. The clincopathological features and pathogenesis of secondary mosaic degeneration of the cornea (anterior crocodile shagreen of Vogt) are described. The structural basis for the normal anterior corneal mosaic pattern seems to lie in the particular arrangement of many prominent collagen lamellae of the anterior stroma that thake an oblique course to gain insertion into Bowman's layer. Since, at normal intraocular pressure, Bowman's layer is under tension, when viewed from the anterior surface the cornea appears smooth. By releasing the tension, however, a reproducible polygonal ridge pattern becomes manifest. It is suggested that a prolonged phthisical state of the eye is one condition wherein the mosaic pattern may become permanent and that, as a secondary event, this is followed by irregular calcification of Bowman's layer which particularly involves the ridges projecting into the epithelium. Biomicroscopically these ridges corresponded to the branching reticular arrangement of the mosaic opacities."}
{"id": "PMID:1079138", "title": "The metabolism of some folates in the rat.", "content": "1. A number of folates labelles with 14-C were administered orally to rats, at various doses, and urinary, faecal and hepatic folates examined. 2. 10-Formylpteroylmonoglutamic acid ( 10CHO--PGA) entered the folate pool very slowly, and is thought to be relatively ineffective in nutrition. 3. 10-Formyl[2-14-C]tetrahydrofolic acid (10CHO--[2-14-C]THF) entered the folate pool very rapidly. 5-Methyl[2-14-C]tetrahydrofolate (5CH3--[2-14-C]THF) was the major urinary folate. 4. 5-Formyl[2-14-C]tetrahydrofolic acid (5CHO--[2-14-C]THF) entered the folate pool only to a small extent. 5CHO--[2-14-C]THF, given intravenously, produced no urinary 5CH3--[2-14-C]THF in the first 6 h. 5. 5,10-Methylidyne[2-14-C]tetrahydrofolic acid was metabolized to an extent which was dependant on the dose. At doses of 3 and 30 mug/kg body-weight, 5CH3--[2-14-C]THF represented 5-4 and 20% respectively of urinary folates anf for 10CHO--[2-14]PGA, the values were 16% of total urinary folates after the higher dose, and 78-5% after the lower dose. 6. Results obtained for the metabolism of 5CH3--THF varied depending on the position of the labelling: 5-14-CH3-THF gave no labelled urinary folate, the methyl group being lost rapidly. When 5CH3--[2-14-C]THF was given, it appeared as the major urinary folate. 7. Folates found in the liver after oral administration of labelled folates were identified by thin-layer chromatography; only folate monoglutamates were identified.", "contents": "The metabolism of some folates in the rat. 1. A number of folates labelles with 14-C were administered orally to rats, at various doses, and urinary, faecal and hepatic folates examined. 2. 10-Formylpteroylmonoglutamic acid ( 10CHO--PGA) entered the folate pool very slowly, and is thought to be relatively ineffective in nutrition. 3. 10-Formyl[2-14-C]tetrahydrofolic acid (10CHO--[2-14-C]THF) entered the folate pool very rapidly. 5-Methyl[2-14-C]tetrahydrofolate (5CH3--[2-14-C]THF) was the major urinary folate. 4. 5-Formyl[2-14-C]tetrahydrofolic acid (5CHO--[2-14-C]THF) entered the folate pool only to a small extent. 5CHO--[2-14-C]THF, given intravenously, produced no urinary 5CH3--[2-14-C]THF in the first 6 h. 5. 5,10-Methylidyne[2-14-C]tetrahydrofolic acid was metabolized to an extent which was dependant on the dose. At doses of 3 and 30 mug/kg body-weight, 5CH3--[2-14-C]THF represented 5-4 and 20% respectively of urinary folates anf for 10CHO--[2-14]PGA, the values were 16% of total urinary folates after the higher dose, and 78-5% after the lower dose. 6. Results obtained for the metabolism of 5CH3--THF varied depending on the position of the labelling: 5-14-CH3-THF gave no labelled urinary folate, the methyl group being lost rapidly. When 5CH3--[2-14-C]THF was given, it appeared as the major urinary folate. 7. Folates found in the liver after oral administration of labelled folates were identified by thin-layer chromatography; only folate monoglutamates were identified."}
{"id": "PMID:1079139", "title": "Membrane biosynthesis in the frog retina: opsin transport in the photoreceptor cell.", "content": "Rhodopsin biosynthesis and transport in the photoreceptor cell have been analyzed by subcellular fractionation of frog retinas after short periods of radioactive amino acid incorporation in vivo. Labelled membrane proteins were identified by autoradiography or sodium dodecyl sulfate-polyacrylamide gels. One of the most intensly labeled proteins in retina had a molecular weight comparable to opsin isolated from purified rod outer segments (ROS). Incorporation of label into this protein was rapid; the relative specific activity then diminished after the first 2 hr as radioactivity was transferred from retinal subcellular fractions to ROS. The kinetics of this transfer resembled rates previously observed by Hall et al. (Hall, M. O., Bok, D., and Bacharach, A.C.E. (1969), J. Mol. Biol. 45, 397). To identify the rapidly labeled protein as opsin we devised a new technique of two-dimensional immunoelectrophoresis of detergent solubilized membrane proteins. Antibodies were prepared against both whole ROS and opsin. After initial separation of retinal proteins on sodium dodecyl sulfate-polyacrylamide gels, a second dimension of electrophoresis in agarose, containing antisera, resulted in the formation of specific immunoprecipitates. Immunochemical analysis of all membranous and soluble retinal subcellular fractions indicated that newly synthesized opsin was membrane bound upon completion of synthesis. At no period of incorporation was a soluble form of newly synthesized opsin detectable. On this basis, we suggest that this protein is apparently transported as a water-insoluble membrane-bound molecule through the cytoplasm or along membranes of the inner segment to its assembly site near the base of the outer segment.", "contents": "Membrane biosynthesis in the frog retina: opsin transport in the photoreceptor cell. Rhodopsin biosynthesis and transport in the photoreceptor cell have been analyzed by subcellular fractionation of frog retinas after short periods of radioactive amino acid incorporation in vivo. Labelled membrane proteins were identified by autoradiography or sodium dodecyl sulfate-polyacrylamide gels. One of the most intensly labeled proteins in retina had a molecular weight comparable to opsin isolated from purified rod outer segments (ROS). Incorporation of label into this protein was rapid; the relative specific activity then diminished after the first 2 hr as radioactivity was transferred from retinal subcellular fractions to ROS. The kinetics of this transfer resembled rates previously observed by Hall et al. (Hall, M. O., Bok, D., and Bacharach, A.C.E. (1969), J. Mol. Biol. 45, 397). To identify the rapidly labeled protein as opsin we devised a new technique of two-dimensional immunoelectrophoresis of detergent solubilized membrane proteins. Antibodies were prepared against both whole ROS and opsin. After initial separation of retinal proteins on sodium dodecyl sulfate-polyacrylamide gels, a second dimension of electrophoresis in agarose, containing antisera, resulted in the formation of specific immunoprecipitates. Immunochemical analysis of all membranous and soluble retinal subcellular fractions indicated that newly synthesized opsin was membrane bound upon completion of synthesis. At no period of incorporation was a soluble form of newly synthesized opsin detectable. On this basis, we suggest that this protein is apparently transported as a water-insoluble membrane-bound molecule through the cytoplasm or along membranes of the inner segment to its assembly site near the base of the outer segment."}
{"id": "PMID:1079140", "title": "Optical diffraction study of muscle fibers.", "content": "Skeletal muscle fibers act as a one-dimensional grating to electromagnetic waves in the optical frequency range. Results are presented of the optical diffraction study of frog sartorius muscle fibers. 1. A simple theoretical consideration predicts that, at a sarcomere length near 3 mum, the second-order reflection is contributed only by the thin filaments due to the destructive interference between scattered rays from thick filaments. At that sarcomere length, both the first-order and the third-order reflections are contributed by both kinds of filaments. 2. Intensity measurements of diffraction lines of muscle fibers at rest at various sarcomere lengths confirmed the validity of our theoretical prediction.3. The isometric contraction of skinned fibers was realized by the phoretic injection of Ca2+ from a micropipette. During the Ca2+ injection intensity drops of the diffraction lines were observed. At a very narrow range of sarcomere lengths near 3 mum, the intensity drop of the second-order diffraction line became very small on Ca2+ injection, whereas the intensity drops of both the first-order and the third-order diffraction lines were very large. 4. A theoretical analysis concludes that the intensity drops on Ca2'njection are solely due to small random fluctuations of the position of thick filaments in the sarcomeres.", "contents": "Optical diffraction study of muscle fibers. Skeletal muscle fibers act as a one-dimensional grating to electromagnetic waves in the optical frequency range. Results are presented of the optical diffraction study of frog sartorius muscle fibers. 1. A simple theoretical consideration predicts that, at a sarcomere length near 3 mum, the second-order reflection is contributed only by the thin filaments due to the destructive interference between scattered rays from thick filaments. At that sarcomere length, both the first-order and the third-order reflections are contributed by both kinds of filaments. 2. Intensity measurements of diffraction lines of muscle fibers at rest at various sarcomere lengths confirmed the validity of our theoretical prediction.3. The isometric contraction of skinned fibers was realized by the phoretic injection of Ca2+ from a micropipette. During the Ca2+ injection intensity drops of the diffraction lines were observed. At a very narrow range of sarcomere lengths near 3 mum, the intensity drop of the second-order diffraction line became very small on Ca2+ injection, whereas the intensity drops of both the first-order and the third-order diffraction lines were very large. 4. A theoretical analysis concludes that the intensity drops on Ca2'njection are solely due to small random fluctuations of the position of thick filaments in the sarcomeres."}
{"id": "PMID:1079141", "title": "X-ray diffraction studies of retinal rods. I. Structure of the disc membrane, effect of illumination.", "content": "The structure of the retinal rod disc membrane and its modifications upon bleaching have been studied by X-ray diffraction. Three types of preparations are used: functioning isolated from retina, isolated rods from frog retina, oriented by a magnetic field, and stacked discs from cattle retina. X-rays are detected by a position-sensitive linear counter. Diffraction spectra are obtained in 10-100 s. The electron density profile favors models where the rhodopsin molecule spans the whole thickness of the membrane. Upon bleaching, a small increase of electron density appears instantly at the cytoplasmic edge of the membrane. In the intact retina this structural change is accompanied by disorder and slow swelling reactions which are not observed in the isolated rod outer segment. The diffraction signal arising from the protein distribution in the plane of the membrane has been reinvestigated carefully. Patterns identical to those of Blasie (Blaise (1969) J. Mol. Biol. 39, 407 and Blaise (1972) Biophys. J. 12, 191) can be obtained but these are shown to be dominated by artefacts. The actual signal is a single broad band around (55 A)-1, upon which bleaching has a negligible effect. No measurable displacement of rhodopsin in the thickness of the membrane occurs upon bleaching. Temperature effects on the protein distribution are found to be large only for disc membranes from cattle retina. In this material from a warm-blooded animal those effects are correlated with the occurrence, upon lowering the temperature, of a partial phase transition of the paraffin chains of the lipids. The position and the slope of the transition are not sensitive to bleaching.", "contents": "X-ray diffraction studies of retinal rods. I. Structure of the disc membrane, effect of illumination. The structure of the retinal rod disc membrane and its modifications upon bleaching have been studied by X-ray diffraction. Three types of preparations are used: functioning isolated from retina, isolated rods from frog retina, oriented by a magnetic field, and stacked discs from cattle retina. X-rays are detected by a position-sensitive linear counter. Diffraction spectra are obtained in 10-100 s. The electron density profile favors models where the rhodopsin molecule spans the whole thickness of the membrane. Upon bleaching, a small increase of electron density appears instantly at the cytoplasmic edge of the membrane. In the intact retina this structural change is accompanied by disorder and slow swelling reactions which are not observed in the isolated rod outer segment. The diffraction signal arising from the protein distribution in the plane of the membrane has been reinvestigated carefully. Patterns identical to those of Blasie (Blaise (1969) J. Mol. Biol. 39, 407 and Blaise (1972) Biophys. J. 12, 191) can be obtained but these are shown to be dominated by artefacts. The actual signal is a single broad band around (55 A)-1, upon which bleaching has a negligible effect. No measurable displacement of rhodopsin in the thickness of the membrane occurs upon bleaching. Temperature effects on the protein distribution are found to be large only for disc membranes from cattle retina. In this material from a warm-blooded animal those effects are correlated with the occurrence, upon lowering the temperature, of a partial phase transition of the paraffin chains of the lipids. The position and the slope of the transition are not sensitive to bleaching."}
{"id": "PMID:1079142", "title": "X-ray diffraction studies of retinal rods. II. Light effect on the osmotic properties.", "content": "A fast X-ray diffraction technique has been used to study the osmotic reaction of frog rod outer segments to bleaching and to changes in the osmolarity and composition of the outer medium. Dissected excitable retina and isolated outer segments have been used. Upon bleaching in isotonic Ringer only a small transient diminution of the disc repeat distance is observed in isolated rods. Disorder and slow swelling reactions are also observed in the intact cells. In calcium-free Ringer the light-induced shrinkage is considerably enhanced. In the intact cell this is interpretable as due to the switching off of a large sodium dark current. The persistence of the effect in isolated outer segment suggests the existence of an active ionic efflux from this part of the cell. Upon hypotonic shock, bleached rods swell more than dark-adapted ones. The difference, however, appears only in a slow component of the osmotic kinetics, a few minutes after the shock. Upon hyertonic shock, part of the rods, even in the \"intact\" excitable retina, appear to be leaking. Those cells which are intact are impermeable to all the solutes added to increase the osmolarity: NaC1, KC1, Sucrose, Melezitose. No light dependence of the response to a hyperosmotic NaC1 shock is detectable. The discs are osmotically reactive, even when the outer cell membrane of the rods is leaking. Assuming the discs to be perfect osmometers, a thickness of 20 plus or minus 5 A is estimated for the liquid layer inside the discs.", "contents": "X-ray diffraction studies of retinal rods. II. Light effect on the osmotic properties. A fast X-ray diffraction technique has been used to study the osmotic reaction of frog rod outer segments to bleaching and to changes in the osmolarity and composition of the outer medium. Dissected excitable retina and isolated outer segments have been used. Upon bleaching in isotonic Ringer only a small transient diminution of the disc repeat distance is observed in isolated rods. Disorder and slow swelling reactions are also observed in the intact cells. In calcium-free Ringer the light-induced shrinkage is considerably enhanced. In the intact cell this is interpretable as due to the switching off of a large sodium dark current. The persistence of the effect in isolated outer segment suggests the existence of an active ionic efflux from this part of the cell. Upon hypotonic shock, bleached rods swell more than dark-adapted ones. The difference, however, appears only in a slow component of the osmotic kinetics, a few minutes after the shock. Upon hyertonic shock, part of the rods, even in the \"intact\" excitable retina, appear to be leaking. Those cells which are intact are impermeable to all the solutes added to increase the osmolarity: NaC1, KC1, Sucrose, Melezitose. No light dependence of the response to a hyperosmotic NaC1 shock is detectable. The discs are osmotically reactive, even when the outer cell membrane of the rods is leaking. Assuming the discs to be perfect osmometers, a thickness of 20 plus or minus 5 A is estimated for the liquid layer inside the discs."}
{"id": "PMID:1079143", "title": "Excited states of photosynthetic reaction centers at low recox potentials.", "content": "In preparations of photochemical reaction centers from Rhodopseudomonas spheroides R-26, lowering the recox potential so as to reduce the primary electron acceptor prevents the photochemical transfer of an electron from bacteriochlorophyll to the acceptor.  Measuring absorbance changes under these conditions, we found that a 20-ns actinic flash converts the reaction center to a new state,  P-F, which then decays with a half-time that is between 1 and 10 ns at 295 degrees K.  At 25 degrees K, the decay half-time is approx.  20 ns.  The quantum yield of state P-F appears to be near 1.0, both at 295 and at 15 degrees K.  State P-F could be an intermediate in the photochemical electron-transfer reaction which occurs when the acceptor is in the oxidized form.  Following the decay of state P-F, we detected another state, P-R, with a decay half-time of 6 mus at 295 degrees K and 120 mus at 15 degrees K.  The quantum yield of state P-R is approx. 0.1 at 295 degrees K, but rises to a value nearer 1.0 at 15 degrees K.  The kinetics and quantum yields are consistent with the view that state P-R forms from P-F.  State P-R seems likely to be a side-product, rather than an intermediate in the electron-transfer process.  The decay kinetics indicate that state P-F cannot be identical with the lowest excited singlet state of the reaction center.  One of the two states, P-F or P-R, probably is the lowest excited triplet state of the reaction center, but it remains unclear which one.", "contents": "Excited states of photosynthetic reaction centers at low recox potentials. In preparations of photochemical reaction centers from Rhodopseudomonas spheroides R-26, lowering the recox potential so as to reduce the primary electron acceptor prevents the photochemical transfer of an electron from bacteriochlorophyll to the acceptor.  Measuring absorbance changes under these conditions, we found that a 20-ns actinic flash converts the reaction center to a new state,  P-F, which then decays with a half-time that is between 1 and 10 ns at 295 degrees K.  At 25 degrees K, the decay half-time is approx.  20 ns.  The quantum yield of state P-F appears to be near 1.0, both at 295 and at 15 degrees K.  State P-F could be an intermediate in the photochemical electron-transfer reaction which occurs when the acceptor is in the oxidized form.  Following the decay of state P-F, we detected another state, P-R, with a decay half-time of 6 mus at 295 degrees K and 120 mus at 15 degrees K.  The quantum yield of state P-R is approx. 0.1 at 295 degrees K, but rises to a value nearer 1.0 at 15 degrees K.  The kinetics and quantum yields are consistent with the view that state P-R forms from P-F.  State P-R seems likely to be a side-product, rather than an intermediate in the electron-transfer process.  The decay kinetics indicate that state P-F cannot be identical with the lowest excited singlet state of the reaction center.  One of the two states, P-F or P-R, probably is the lowest excited triplet state of the reaction center, but it remains unclear which one."}
{"id": "PMID:1079144", "title": "Diffusion resistance of endothelium and stroma of bullfrog cornea determined by potential response to K+.", "content": "Corneas of bullfrog (Rana catesbeiana) were mounted between lucite chambers. A four-electrode system was used and the potential difference (PD) and the electrical resistance were measured. In intact corneas, the PD averaged 25 mV (acqueous side positive) and the electrical resistance 1.5 kQ - cm2. perfusion of the aqueous side with high K+ solutions resulted in a marked decrease in PD and a drop in the electrical resistance. Scraping the epithelium (leaving the stroma plus endothelium) resulted in a drop of the PD to about zero and a decrease in electrical resistance to about 0.1 kQ - CM2 and a very small PD response to a marked elevation of the K+ concentration on the aqueous side. On the basis of the above, it is obvious that the large delta PD in intact corneas, due to elevation of the K+ concentration, must be due to K+ diffusing from the aqueous side across the endothelium and stroma and reaching the epithelium. The duration of the PD response is therefore a measure of the resistance to diffusion of the stroma plus endothelium. A quantitiative analysis shows that under in vitro conditions the resistance of the endothelium plus stroma to the diffusion of ions is very low.", "contents": "Diffusion resistance of endothelium and stroma of bullfrog cornea determined by potential response to K+. Corneas of bullfrog (Rana catesbeiana) were mounted between lucite chambers. A four-electrode system was used and the potential difference (PD) and the electrical resistance were measured. In intact corneas, the PD averaged 25 mV (acqueous side positive) and the electrical resistance 1.5 kQ - cm2. perfusion of the aqueous side with high K+ solutions resulted in a marked decrease in PD and a drop in the electrical resistance. Scraping the epithelium (leaving the stroma plus endothelium) resulted in a drop of the PD to about zero and a decrease in electrical resistance to about 0.1 kQ - CM2 and a very small PD response to a marked elevation of the K+ concentration on the aqueous side. On the basis of the above, it is obvious that the large delta PD in intact corneas, due to elevation of the K+ concentration, must be due to K+ diffusing from the aqueous side across the endothelium and stroma and reaching the epithelium. The duration of the PD response is therefore a measure of the resistance to diffusion of the stroma plus endothelium. A quantitiative analysis shows that under in vitro conditions the resistance of the endothelium plus stroma to the diffusion of ions is very low."}
{"id": "PMID:1079145", "title": "Pulsed nuclear magnetic resonance study of 17-O, 2-D, and 1-H of water in frog striated muscle.", "content": "Whole gastrocnemius muscles were incubated in Ringer's solution enriched with H2-17O; the paired contralateral gastrocnemius muscles were incubated in a similar solution enriched with deuterons, as well. Subsequently, the longitudinal relaxation times (T1) were measured 17-O, 2-D, and 1-H, both at 8.1 MHz and at 4.3 MHz. The results indicate that: (a) the absolute values of T1 characterizing the three nuclides are different in muscle and pure water. (b) the longitudinal relaxation rates of all three have an identical frequency dependence over the range studied, (c) the ratio (T1)2D/(T1)17ois the same in muscle water and pure water, while the ratio (T1)1H/(T1)17o is 2.1 times greater in pure water than it is in muscle water, and (d) 30-49 percent substitution of 2-D for 1-H has very little effect on the spin-lattice relaxation of tissue water protons. These data suggest that muscle water is in rapid exchange between a small fraction of immobilized molecules and a large fraction of free water. The results render unlikely the possibility that hypothetical ordering of muscle water significantly contributes to its longitudinal relaxation.", "contents": "Pulsed nuclear magnetic resonance study of 17-O, 2-D, and 1-H of water in frog striated muscle. Whole gastrocnemius muscles were incubated in Ringer's solution enriched with H2-17O; the paired contralateral gastrocnemius muscles were incubated in a similar solution enriched with deuterons, as well. Subsequently, the longitudinal relaxation times (T1) were measured 17-O, 2-D, and 1-H, both at 8.1 MHz and at 4.3 MHz. The results indicate that: (a) the absolute values of T1 characterizing the three nuclides are different in muscle and pure water. (b) the longitudinal relaxation rates of all three have an identical frequency dependence over the range studied, (c) the ratio (T1)2D/(T1)17ois the same in muscle water and pure water, while the ratio (T1)1H/(T1)17o is 2.1 times greater in pure water than it is in muscle water, and (d) 30-49 percent substitution of 2-D for 1-H has very little effect on the spin-lattice relaxation of tissue water protons. These data suggest that muscle water is in rapid exchange between a small fraction of immobilized molecules and a large fraction of free water. The results render unlikely the possibility that hypothetical ordering of muscle water significantly contributes to its longitudinal relaxation."}
{"id": "PMID:1079146", "title": "[The effect of strychnine on the muscle receptors of cold-blooded animals].", "content": "Experiments were conducted on frogs. A study was made of the functional condition of the muscle receptors by the method of recording of spontaneous and afferent impulsation induced by the strain of the gastrocnemius in the peripheral section of the sciatic nerve or in the IX-X posterior spinal cord roots. It was revealed that strychnine administered into the general circulation and applied directly into the muscle produced a reduction and that applied to the spinal cord--on the contrary--caused an increase in the impulse generation in the muscle receptors.", "contents": "[The effect of strychnine on the muscle receptors of cold-blooded animals]. Experiments were conducted on frogs. A study was made of the functional condition of the muscle receptors by the method of recording of spontaneous and afferent impulsation induced by the strain of the gastrocnemius in the peripheral section of the sciatic nerve or in the IX-X posterior spinal cord roots. It was revealed that strychnine administered into the general circulation and applied directly into the muscle produced a reduction and that applied to the spinal cord--on the contrary--caused an increase in the impulse generation in the muscle receptors."}
{"id": "PMID:1079147", "title": "Hypothesis: differentiation of the human lymphoid system based on cell surface markers.", "content": "Human lymphocytes can be separated into distinct populations based upon receptors on their cell surface. Thymus-derived (T-cell) lymphocytes can be identified by their ability to form rosetts with sheep erythrocytes (SRBC); bone marrow-derived (B-cell) lymphocytes bear characteristic surface markers for immunoglobulin, complement, and the Fc portion of IgG. Recently, populations of lymphocytes having either multiple markers or no detectable markers (null cells) have been observed. Based on studies of cell surface markers, a scheme is proposed that expands the known differentiation of the lymphod cell to include subpopulations which represent developmental stages. It is suggested that lymphocyte maturation involves alloantigenic changes in a circulating stem cell-drived nill cell, leading to a cell bearing markers for both T- and B-cells. It is from this latter cell that the classic T- and B-cells ultimately arise. Maturational defects which may explain the origin of primary lymphoproliferative diseases are discussed.", "contents": "Hypothesis: differentiation of the human lymphoid system based on cell surface markers. Human lymphocytes can be separated into distinct populations based upon receptors on their cell surface. Thymus-derived (T-cell) lymphocytes can be identified by their ability to form rosetts with sheep erythrocytes (SRBC); bone marrow-derived (B-cell) lymphocytes bear characteristic surface markers for immunoglobulin, complement, and the Fc portion of IgG. Recently, populations of lymphocytes having either multiple markers or no detectable markers (null cells) have been observed. Based on studies of cell surface markers, a scheme is proposed that expands the known differentiation of the lymphod cell to include subpopulations which represent developmental stages. It is suggested that lymphocyte maturation involves alloantigenic changes in a circulating stem cell-drived nill cell, leading to a cell bearing markers for both T- and B-cells. It is from this latter cell that the classic T- and B-cells ultimately arise. Maturational defects which may explain the origin of primary lymphoproliferative diseases are discussed."}
{"id": "PMID:1079150", "title": "A genetic study of bipolar affective disorder.", "content": "A group of 46 bipolar probands and their first degree relatives were studied. A high rate of affective disorder (19.6 per cent) was found, including both unipolar (13.2 percent) and bipolar (6.4 per cent) types, with females predominating (3 : 1). The presence of four fatherson pairs suffering from affective disorder made the hypothesis of X-linked dominance untenable. Results compatible with polygenic inheritance were found, using both Slater's and Falconer's methods. There was no evidence for assortative mating or for increased total number of females (both well and ill) among first degree relatives. The probands and affectively ill first degree relatives who have died show an alarmingly high rate of suicide (46 per cent). Other forms of mental disorder, including alcoholism, were no more common than in the rest of the community.", "contents": "A genetic study of bipolar affective disorder. A group of 46 bipolar probands and their first degree relatives were studied. A high rate of affective disorder (19.6 per cent) was found, including both unipolar (13.2 percent) and bipolar (6.4 per cent) types, with females predominating (3 : 1). The presence of four fatherson pairs suffering from affective disorder made the hypothesis of X-linked dominance untenable. Results compatible with polygenic inheritance were found, using both Slater's and Falconer's methods. There was no evidence for assortative mating or for increased total number of females (both well and ill) among first degree relatives. The probands and affectively ill first degree relatives who have died show an alarmingly high rate of suicide (46 per cent). Other forms of mental disorder, including alcoholism, were no more common than in the rest of the community."}
{"id": "PMID:1079153", "title": "Haematemesis: a new syndrome?", "content": "Three patients presented with symptoms suggesting a Mallory-Weiss tear. Endoscopy showed a localized, clearly demarcated area of bright red mucosa near the gastro-oesophageal junction; this was thought to have arisen by retrograde intussusception of the stomach during vomiting or retching and may have caused the haemorrhage.", "contents": "Haematemesis: a new syndrome? Three patients presented with symptoms suggesting a Mallory-Weiss tear. Endoscopy showed a localized, clearly demarcated area of bright red mucosa near the gastro-oesophageal junction; this was thought to have arisen by retrograde intussusception of the stomach during vomiting or retching and may have caused the haemorrhage."}
{"id": "PMID:1079158", "title": "Uptake of local anaesthetics: movement of ethanol into frog sciatic nerve.", "content": "The changes of nerve action potential with time of the excised bullfrog sciaticperoneal nerve were measured at 24 degrees C with the nerve immersed in oxygenated mineral oil containing various concentrations of ethanol. One series of measurements was made with intact nerve and a range of about 20 to 110 millimolar ethanol. Another series was made with nerves in which the epineural sheath was cut open longitudinally and using a range of about 15 to 51 millimolar ethanol. The results were analysed by means of simple diffusion theory, and below applied ethanol concentrations of about 70 millimolar the latent periods and times to total block fitted the theoretical pattern well. Disagreements between theory and measurement of previous workers is explainable by their neglecting to recognize that nerve bundles do not extend to the nerve periphery. It was found that the minimum oil concentration of ethanol capable of producing total block is 15 millimolar. Above 70 millimolar, behaviour was observed consistent with nerve damage. It was found possible, by measuring the ratio latent period: time to total block, to separate the relative contribution of the epineural sheath and epineurium as a diffusion barrier to ethanol from that of the perineurium and perilemma.", "contents": "Uptake of local anaesthetics: movement of ethanol into frog sciatic nerve. The changes of nerve action potential with time of the excised bullfrog sciaticperoneal nerve were measured at 24 degrees C with the nerve immersed in oxygenated mineral oil containing various concentrations of ethanol. One series of measurements was made with intact nerve and a range of about 20 to 110 millimolar ethanol. Another series was made with nerves in which the epineural sheath was cut open longitudinally and using a range of about 15 to 51 millimolar ethanol. The results were analysed by means of simple diffusion theory, and below applied ethanol concentrations of about 70 millimolar the latent periods and times to total block fitted the theoretical pattern well. Disagreements between theory and measurement of previous workers is explainable by their neglecting to recognize that nerve bundles do not extend to the nerve periphery. It was found that the minimum oil concentration of ethanol capable of producing total block is 15 millimolar. Above 70 millimolar, behaviour was observed consistent with nerve damage. It was found possible, by measuring the ratio latent period: time to total block, to separate the relative contribution of the epineural sheath and epineurium as a diffusion barrier to ethanol from that of the perineurium and perilemma."}
{"id": "PMID:1079162", "title": "Bloodless open heart surgery with atraumatic extracorporeal circulation.", "content": "With careful refinements in the pump oxygenator and a nonblood prime, bloodless open heart surgery may be performed almost routinely. In our series these measures reduced blood trauma, with a remarkable preservation of blood elements, especially platelets, and a corresponding elimination of postoperative bleeding. The mean hematocrit value decreased from 38 to 27% and recovered to 33% in the first 3 hours of postoperative diuresis. Mannitol and furosemide were rarely needed. Of 61 adult patients whp underwent open heart surgery for aortocoronary bypass or valve replacement, the last 43 had a bloodless procedure. Of the 43, 26 (60%) required no bank blood postoperatively (in the operating room or the intensive care unit). In these 26 the operative mortality was 4% (1 patient). Bloodless techniques are invaluable during periods of bank blood shortage. They avoid the dangers of hepatitis and transfusion reaction, and they may minimize the incidence of postperfusion lung syndrome as well as renal complications.", "contents": "Bloodless open heart surgery with atraumatic extracorporeal circulation. With careful refinements in the pump oxygenator and a nonblood prime, bloodless open heart surgery may be performed almost routinely. In our series these measures reduced blood trauma, with a remarkable preservation of blood elements, especially platelets, and a corresponding elimination of postoperative bleeding. The mean hematocrit value decreased from 38 to 27% and recovered to 33% in the first 3 hours of postoperative diuresis. Mannitol and furosemide were rarely needed. Of 61 adult patients whp underwent open heart surgery for aortocoronary bypass or valve replacement, the last 43 had a bloodless procedure. Of the 43, 26 (60%) required no bank blood postoperatively (in the operating room or the intensive care unit). In these 26 the operative mortality was 4% (1 patient). Bloodless techniques are invaluable during periods of bank blood shortage. They avoid the dangers of hepatitis and transfusion reaction, and they may minimize the incidence of postperfusion lung syndrome as well as renal complications."}
{"id": "PMID:1079163", "title": "Quantitation of cell-mediated immunity: responses to dinitrochlorobenzene and ubiquitous antigens.", "content": "T-lymphocyte immune capacity in man was assessed semiquantitatively by two in vivo procedures: the primary type of response to dinitrochlorobenzene and the secondary type of response, representing memory, to a group of five uniquitous antigens. Controlling for degree of illness proved important in assessing immune capacity in specific diseases; thus, the number of responders and mean score of semiquantitated responses was significantly lower in groups of patients with cancer and multisystem autoimmune disease when comparisons were made with healthy persons, but less so when comparisons were made with a group of subjects with other incapacitating diseases. A notable finding was the lack of correlation in the results of tests of cell-mediated immunity between the two procedures described. Depressed cell-mediated immunity shown in multisystem autoimmune disease is relevant to both predisposition to infection and the postulated role of thymic dysfunction in the pathogenesis of autoimmunity.", "contents": "Quantitation of cell-mediated immunity: responses to dinitrochlorobenzene and ubiquitous antigens. T-lymphocyte immune capacity in man was assessed semiquantitatively by two in vivo procedures: the primary type of response to dinitrochlorobenzene and the secondary type of response, representing memory, to a group of five uniquitous antigens. Controlling for degree of illness proved important in assessing immune capacity in specific diseases; thus, the number of responders and mean score of semiquantitated responses was significantly lower in groups of patients with cancer and multisystem autoimmune disease when comparisons were made with healthy persons, but less so when comparisons were made with a group of subjects with other incapacitating diseases. A notable finding was the lack of correlation in the results of tests of cell-mediated immunity between the two procedures described. Depressed cell-mediated immunity shown in multisystem autoimmune disease is relevant to both predisposition to infection and the postulated role of thymic dysfunction in the pathogenesis of autoimmunity."}
{"id": "PMID:1079175", "title": "On electrical condution in living bone.", "content": "Despite the effectiveness of electrical currents in enhancing bone repair, there is little information in the literature on electrical parameters per se. Very little is known about the nature of the conduction mechanism or the current path between the electrodes. Without a better understanding it is difficult to establish meaningful hypotheses at the cellular level and to design relevant experimental protocols. In the present work, a first attempt is made at an in vivo delineation of the current-voltage relationship in the medullary area between two platinum electrodes embedded in the femur, by one of the techniques generally known to stimulate bone growth. At potential differences of less than 1 volt, a rather good ohmic dependence is observed, with an approximate specific resistance of 2 to 5 times 10-5 ohms/cm. At potentials higher than 1 volt, electrolytic processes appear to predominate and there is increasing non-linearity. Experimental techniques involving the adjustment of current through bone tissue assuming an ohmic dependence with little or no associated polarization effects are valid and certainly warrant further investigation.", "contents": "On electrical condution in living bone. Despite the effectiveness of electrical currents in enhancing bone repair, there is little information in the literature on electrical parameters per se. Very little is known about the nature of the conduction mechanism or the current path between the electrodes. Without a better understanding it is difficult to establish meaningful hypotheses at the cellular level and to design relevant experimental protocols. In the present work, a first attempt is made at an in vivo delineation of the current-voltage relationship in the medullary area between two platinum electrodes embedded in the femur, by one of the techniques generally known to stimulate bone growth. At potential differences of less than 1 volt, a rather good ohmic dependence is observed, with an approximate specific resistance of 2 to 5 times 10-5 ohms/cm. At potentials higher than 1 volt, electrolytic processes appear to predominate and there is increasing non-linearity. Experimental techniques involving the adjustment of current through bone tissue assuming an ohmic dependence with little or no associated polarization effects are valid and certainly warrant further investigation."}
{"id": "PMID:1079179", "title": "Role of 11 beta-hydroxyprogesterone in biosynthesis of corticosterone from progesterone in guinea pig adrenals.", "content": "Slices of male guinea pig adrenals were incubated with trace amount of 3-H7 alpha-progesterone and the percentage of incorporation of 3-H into various steroids at various intervals was measured. It was found that 3-H accumulates predominately in 11 beta-hydroxyprogesterone as compared to DOC. This indicates that, in guinea pig adrenals, 11 beta-hydroxyprogesterone plays a dominant role in a formation of corticosterone from progesterone. The observed sequence of changes in progesterone label accumulation in 17-hydroxycompounds indicates they pathway of cortisol synthesis through the intermediate formation of 17-hydroxyprogesterone and 11-deoxycortisol.", "contents": "Role of 11 beta-hydroxyprogesterone in biosynthesis of corticosterone from progesterone in guinea pig adrenals. Slices of male guinea pig adrenals were incubated with trace amount of 3-H7 alpha-progesterone and the percentage of incorporation of 3-H into various steroids at various intervals was measured. It was found that 3-H accumulates predominately in 11 beta-hydroxyprogesterone as compared to DOC. This indicates that, in guinea pig adrenals, 11 beta-hydroxyprogesterone plays a dominant role in a formation of corticosterone from progesterone. The observed sequence of changes in progesterone label accumulation in 17-hydroxycompounds indicates they pathway of cortisol synthesis through the intermediate formation of 17-hydroxyprogesterone and 11-deoxycortisol."}
{"id": "PMID:1079180", "title": "Iodine accumulating ability as generalized porperty of cells of endodermal origin.", "content": "Embryonal trachea transplanted in the spleen of adult rats accumulates iodine. The uptake of iodine does not take place in all of the cells, but iodination centers develop and the iodinated cells detach themselves to the lumen thus resembling the holocrine secreation; The results of these experiments indicate that the uptake of iodine appears to be a generalized property of the endoderm.", "contents": "Iodine accumulating ability as generalized porperty of cells of endodermal origin. Embryonal trachea transplanted in the spleen of adult rats accumulates iodine. The uptake of iodine does not take place in all of the cells, but iodination centers develop and the iodinated cells detach themselves to the lumen thus resembling the holocrine secreation; The results of these experiments indicate that the uptake of iodine appears to be a generalized property of the endoderm."}
{"id": "PMID:1079181", "title": "Effect of estradiol on the activity of serotonin N-acetyltransferase in the rat epiphysis.", "content": "Administration of estradiol to ovariectomized females did not affect either the rhythm and basic levels of activity of the enzyme regulating the formation of melatonin in the rat epiphysis-serotonin N-acetyltransferase or the activation of this enzyme by isoproterenol in vivo. Daily administration of estradiol to young rats from birth did not alter the rhythm or activity of serotonin N-acetyltransferase in the epiphysis as estimated on the 8th day of age. The ability of estradiol to suppress noradrenaline activation of adenyl cyclase in epiphysial homogenates and its inability to suppress the same activation of serotonin N-acetyltransferase suggests either that the activation of the latter enzyme by catecholamines could occur by other mechanisms than the adenyl cyclase system or that activation of adenyl cyclase by noradrenaline is not suppressed by estradiol to the same degree in vivo as in vitro.", "contents": "Effect of estradiol on the activity of serotonin N-acetyltransferase in the rat epiphysis. Administration of estradiol to ovariectomized females did not affect either the rhythm and basic levels of activity of the enzyme regulating the formation of melatonin in the rat epiphysis-serotonin N-acetyltransferase or the activation of this enzyme by isoproterenol in vivo. Daily administration of estradiol to young rats from birth did not alter the rhythm or activity of serotonin N-acetyltransferase in the epiphysis as estimated on the 8th day of age. The ability of estradiol to suppress noradrenaline activation of adenyl cyclase in epiphysial homogenates and its inability to suppress the same activation of serotonin N-acetyltransferase suggests either that the activation of the latter enzyme by catecholamines could occur by other mechanisms than the adenyl cyclase system or that activation of adenyl cyclase by noradrenaline is not suppressed by estradiol to the same degree in vivo as in vitro."}
{"id": "PMID:1079182", "title": "Sodium fluoride-induced changes in the hypothalamic neurosecretory system of the spotted owlet, Athene brama Temminck.", "content": "Administration of 10 or 20 mg of sodium fluoride (NaF) induced depletion of neurosecretory material (NSM) from the neurons of the supraoptic (SON) and paraventricular (PVN) nuclei in spotted owlet (Athene brama Temminck). The residual NSM in the perikarya was aranged in caplike paranuclear positions. The NSM in the hypothalamo-hypophysial tract, the zona externa of the anterior median eminence (ME) and in the neural lobe (NL) showed only a partial lossmthe effects of NaF administration on the HNS of the spotted owlet are similar to those induced by such stimulators of ADH secretion as hypertonic saline, morphine and formalin. Hence the histological changes induced by NaF administration in the HNS of the spotted owlet are presumably indicative of increased neurosecretory activity resulting in the augmented secretion of ADH. It is suggested that NaF, like other stressful agents, is a stimulator of ADH secretion.", "contents": "Sodium fluoride-induced changes in the hypothalamic neurosecretory system of the spotted owlet, Athene brama Temminck. Administration of 10 or 20 mg of sodium fluoride (NaF) induced depletion of neurosecretory material (NSM) from the neurons of the supraoptic (SON) and paraventricular (PVN) nuclei in spotted owlet (Athene brama Temminck). The residual NSM in the perikarya was aranged in caplike paranuclear positions. The NSM in the hypothalamo-hypophysial tract, the zona externa of the anterior median eminence (ME) and in the neural lobe (NL) showed only a partial lossmthe effects of NaF administration on the HNS of the spotted owlet are similar to those induced by such stimulators of ADH secretion as hypertonic saline, morphine and formalin. Hence the histological changes induced by NaF administration in the HNS of the spotted owlet are presumably indicative of increased neurosecretory activity resulting in the augmented secretion of ADH. It is suggested that NaF, like other stressful agents, is a stimulator of ADH secretion."}
{"id": "PMID:1079183", "title": "Activity of catecholamine degrading enzymes in rat adrenal medulla and cortex after acute and repeated stress.", "content": "The activity of catecholamine degrading enzymes (catechol-O-methyltransferase--COMT and monoamine oxidase--MAO) in separated adrenal cortex and medulla was measured in rats stressed by a singel or repeated immobilization. In controls, the activity of both enzymes was higher in adrenal cortex than in adrenal medulla. After stress, the activity of COMT in adrenal cortex was significantly decreased, while that in adrenal medulla was significantly increased. Moreover, the animals repeatedly immobilized showed lower COMT activity in both parts of adrenals when compared to first time stressed rats. The activity of MAO was not remarkably changed, but was increased only in adrenal medulla when expressed per mg protein, the decrease of which resulted from acute stress. It was concluded that the findings of changed activity of catecholamine degrading enzymes, namely a decreased activity of COMT in both adrenal cortex and medulla in repeatedly stressed rats, might show a possible participation of these enzymes in increased catecholamine content in adrenals of repeatedly stressed animals.", "contents": "Activity of catecholamine degrading enzymes in rat adrenal medulla and cortex after acute and repeated stress. The activity of catecholamine degrading enzymes (catechol-O-methyltransferase--COMT and monoamine oxidase--MAO) in separated adrenal cortex and medulla was measured in rats stressed by a singel or repeated immobilization. In controls, the activity of both enzymes was higher in adrenal cortex than in adrenal medulla. After stress, the activity of COMT in adrenal cortex was significantly decreased, while that in adrenal medulla was significantly increased. Moreover, the animals repeatedly immobilized showed lower COMT activity in both parts of adrenals when compared to first time stressed rats. The activity of MAO was not remarkably changed, but was increased only in adrenal medulla when expressed per mg protein, the decrease of which resulted from acute stress. It was concluded that the findings of changed activity of catecholamine degrading enzymes, namely a decreased activity of COMT in both adrenal cortex and medulla in repeatedly stressed rats, might show a possible participation of these enzymes in increased catecholamine content in adrenals of repeatedly stressed animals."}
{"id": "PMID:1079184", "title": "Factors affecting estrogen signalization for FSH secretion in male and adrogenized or normal female rats.", "content": "Factors affecting the FSH feedback mechanism in intact or androgenized females and intact males were studied by ovarian fragment implantation into the spleen. A pronounced sex difference could be observed in the ovarian hypertrophy after transplantation. Males as well as androgenized females as hosts responded more than intact females. On the other hand, ovaries originating either from normal or androgenized rats showed similar enlargement under the spleen capsule, regardless of their origin. It was concluded that the AS females have a deficit in the negative feedback signalization which seems to be due to the differences in hepatic degradation of the estrogens. Nevertheless, a change of the set-point for FSH in androgen sterile rats cannot be excluded.", "contents": "Factors affecting estrogen signalization for FSH secretion in male and adrogenized or normal female rats. Factors affecting the FSH feedback mechanism in intact or androgenized females and intact males were studied by ovarian fragment implantation into the spleen. A pronounced sex difference could be observed in the ovarian hypertrophy after transplantation. Males as well as androgenized females as hosts responded more than intact females. On the other hand, ovaries originating either from normal or androgenized rats showed similar enlargement under the spleen capsule, regardless of their origin. It was concluded that the AS females have a deficit in the negative feedback signalization which seems to be due to the differences in hepatic degradation of the estrogens. Nevertheless, a change of the set-point for FSH in androgen sterile rats cannot be excluded."}
{"id": "PMID:1079186", "title": "Influence of left ventricular function on survival 3 to 4 years after aortocoronary bypass.", "content": "Preoperative left ventricular ejection fraction (EF) was calculated using single plane cineventriculography in 93 patients in whom aortocoronary bypass surgery was performed before July 1970. Preoperative EF (mean plus or minus SEM) was 0.29 plus or minus 0.04 in 9 patients deceased between 2 and 35 mth after operation, significantly lower (p less than 0.001) than EF of 84 patients having survived 3 yr or more (0.55 plus or minus 0.02). The 3- to 4-yr mortality in these patients was 22.2% when preoperative EF was less than 0.50 and 1.75% when it was greater than or equal to 0.50 (p less than 0.01). Likewise, late mortality rose to 43.7% when EF was less than 0.40, compared to 2.6% when it was greater than or equal to 0.40 (p less than 0.005). In 21 patients in this series, left ventricular volumes and EF showed no significant difference before, approximately one year and between 3 and 4 yr after operation. This included 9 patients with normal or near normal contraction and 12 patients with impaired ventricular contraction preoperatively. Thus, in this study, preoperative EF was a major determinant of long-term prognosis after aortocoronary bypass and EF was not improved between 3 and 4 yr postoperatively. These data suggest, then, that in order to enhance survival after aortocoronary bypass, patients should be operated on before significant myocardial damage has occurred.", "contents": "Influence of left ventricular function on survival 3 to 4 years after aortocoronary bypass. Preoperative left ventricular ejection fraction (EF) was calculated using single plane cineventriculography in 93 patients in whom aortocoronary bypass surgery was performed before July 1970. Preoperative EF (mean plus or minus SEM) was 0.29 plus or minus 0.04 in 9 patients deceased between 2 and 35 mth after operation, significantly lower (p less than 0.001) than EF of 84 patients having survived 3 yr or more (0.55 plus or minus 0.02). The 3- to 4-yr mortality in these patients was 22.2% when preoperative EF was less than 0.50 and 1.75% when it was greater than or equal to 0.50 (p less than 0.01). Likewise, late mortality rose to 43.7% when EF was less than 0.40, compared to 2.6% when it was greater than or equal to 0.40 (p less than 0.005). In 21 patients in this series, left ventricular volumes and EF showed no significant difference before, approximately one year and between 3 and 4 yr after operation. This included 9 patients with normal or near normal contraction and 12 patients with impaired ventricular contraction preoperatively. Thus, in this study, preoperative EF was a major determinant of long-term prognosis after aortocoronary bypass and EF was not improved between 3 and 4 yr postoperatively. These data suggest, then, that in order to enhance survival after aortocoronary bypass, patients should be operated on before significant myocardial damage has occurred."}
{"id": "PMID:1079188", "title": "Acetylcholine receptors on Renshaw cells of the rat.", "content": "Experiments have been performed on Renshaw cells of rats to investigate the receptors mediating both the responses to electrophoretically applied cholinergic excitants and the synaptic excitation following stimulation of the ventral roots. The effects of atropine and dihydro-beta-erythroidine, muscarinic and nicotinic antagonists respectively, have been tested on both types of excitation. Most importantly we found that in the rat the ventral root evoked response can be blocked by atropine as well as by DHbetaE. It was not possible to categorise the responses to the cholinergic agents in terms of muscarinic and nicotinic receptors. In addition we have failed the late (muscarinic) excitation seen in the cat.", "contents": "Acetylcholine receptors on Renshaw cells of the rat. Experiments have been performed on Renshaw cells of rats to investigate the receptors mediating both the responses to electrophoretically applied cholinergic excitants and the synaptic excitation following stimulation of the ventral roots. The effects of atropine and dihydro-beta-erythroidine, muscarinic and nicotinic antagonists respectively, have been tested on both types of excitation. Most importantly we found that in the rat the ventral root evoked response can be blocked by atropine as well as by DHbetaE. It was not possible to categorise the responses to the cholinergic agents in terms of muscarinic and nicotinic receptors. In addition we have failed the late (muscarinic) excitation seen in the cat."}
{"id": "PMID:1079189", "title": "The mode of action of 5-hydroxytryptophan in facilitating a stretch reflex in the spinal cat.", "content": "1. Intravenous injection of 5-hydroxytryptophan (5-HTP) excites discharges of gamma motoneurones to extensor muscles of the hind limbs of decerebrate spinal cats and this occurs with a short latency (1-2 min). 2. 5-HTP also facilitates the stretch reflex in the spinal cat but, in contrast, there is a latency of about 10 min with a maximum action after 20 min. 3. Facilitation of the stretch reflex by 5-HTP still occurs when the gamma loop has been opened by cutting all ventral roots supplying the muscle. Thus excitation of gamma motoneurones by 5-HTP is not required for facilitation of the stretch reflex. 4. A possible contribution to the stretch reflex from the increased discharge of gamma motoneurones has been assessed. Selectively removing gamma activity rarely altered the reflex response to stretch and in only 3 out of 15 spinal cats could we conclude that gamma discharges contributed towards facilitation of the stretch reflex by 5-HTP; 5; Methysergide (0.25 mg/kg) blocked completely the facilitation of the stretch reflex caused by 5-HTP in the spinal cat but only partially antagonised the reflex in the decerebrate cat, even when injected intra-arterially close to the lumbar spinal cord. 6. Lysergic acid diethylamide (LSD) was found to mimic the actions of 5-HTP. It facilitated the stretch reflex and excited extensor gamma motoneurones in the spinal animal.", "contents": "The mode of action of 5-hydroxytryptophan in facilitating a stretch reflex in the spinal cat. 1. Intravenous injection of 5-hydroxytryptophan (5-HTP) excites discharges of gamma motoneurones to extensor muscles of the hind limbs of decerebrate spinal cats and this occurs with a short latency (1-2 min). 2. 5-HTP also facilitates the stretch reflex in the spinal cat but, in contrast, there is a latency of about 10 min with a maximum action after 20 min. 3. Facilitation of the stretch reflex by 5-HTP still occurs when the gamma loop has been opened by cutting all ventral roots supplying the muscle. Thus excitation of gamma motoneurones by 5-HTP is not required for facilitation of the stretch reflex. 4. A possible contribution to the stretch reflex from the increased discharge of gamma motoneurones has been assessed. Selectively removing gamma activity rarely altered the reflex response to stretch and in only 3 out of 15 spinal cats could we conclude that gamma discharges contributed towards facilitation of the stretch reflex by 5-HTP; 5; Methysergide (0.25 mg/kg) blocked completely the facilitation of the stretch reflex caused by 5-HTP in the spinal cat but only partially antagonised the reflex in the decerebrate cat, even when injected intra-arterially close to the lumbar spinal cord. 6. Lysergic acid diethylamide (LSD) was found to mimic the actions of 5-HTP. It facilitated the stretch reflex and excited extensor gamma motoneurones in the spinal animal."}
{"id": "PMID:1079190", "title": "Vestibular and somatosensory inflow to the vestibular projection area in the post cruciate dimple region of the cat cerebral cortex.", "content": "In anesthetized cats 251 cells within the cortical vestibular projection area, adjacent to the post-cruciate dimple, were analyzed as to their input characteristics employing extracellular recording techniques. The post cruciate dimple vestibular field, which is located in area 3a, has a high degree of convergence between vestibular and peripheral somatosensory input. The latter is not restricted to muscle afferents but includes cutaneous modalities. The functional significance of this vestibular cortical projection field is discussed.", "contents": "Vestibular and somatosensory inflow to the vestibular projection area in the post cruciate dimple region of the cat cerebral cortex. In anesthetized cats 251 cells within the cortical vestibular projection area, adjacent to the post-cruciate dimple, were analyzed as to their input characteristics employing extracellular recording techniques. The post cruciate dimple vestibular field, which is located in area 3a, has a high degree of convergence between vestibular and peripheral somatosensory input. The latter is not restricted to muscle afferents but includes cutaneous modalities. The functional significance of this vestibular cortical projection field is discussed."}
{"id": "PMID:1079192", "title": "[Changes in the ultrastructure of frog spinal cord motor neurons following low frequency stimulation of afferent fibers].", "content": "Effect of 10 min stimulation on the motoneurones ultrastructure depended on the amplitude of the ventral root biopotentials.  In case of low amplitudes dilated endoplasmic spaces, Golgi complex cisterns were seen.  Mitochondria were swollen and the number of free ribosomes increased.  In the cytoplasm many \"whorl--like\" structures appeared.  In case of high amplitude ventral root biopotentials, alterations were particularly evident in decrease in the ribosomes number, fragmentation of the endoplasmic reticulum.  Electron density of the motoneurons was very low.  After 25 min stimulation, the number of ribosomes increased in the motoneurons, the Golgi complex proliferated, very large mitochondria appeared.  In the dendrites, swollen mitochondria often were adjacent to postsynaptic membranes.  Electron density of the motoneurons was very high.  The ultrastructural alterations of the motoneurons are supposed to be due to a change of cellular metabolism in the conditions of increased functional activity.", "contents": "[Changes in the ultrastructure of frog spinal cord motor neurons following low frequency stimulation of afferent fibers]. Effect of 10 min stimulation on the motoneurones ultrastructure depended on the amplitude of the ventral root biopotentials.  In case of low amplitudes dilated endoplasmic spaces, Golgi complex cisterns were seen.  Mitochondria were swollen and the number of free ribosomes increased.  In the cytoplasm many \"whorl--like\" structures appeared.  In case of high amplitude ventral root biopotentials, alterations were particularly evident in decrease in the ribosomes number, fragmentation of the endoplasmic reticulum.  Electron density of the motoneurons was very low.  After 25 min stimulation, the number of ribosomes increased in the motoneurons, the Golgi complex proliferated, very large mitochondria appeared.  In the dendrites, swollen mitochondria often were adjacent to postsynaptic membranes.  Electron density of the motoneurons was very high.  The ultrastructural alterations of the motoneurons are supposed to be due to a change of cellular metabolism in the conditions of increased functional activity."}
{"id": "PMID:1079193", "title": "The part played by cell-mediated immunity in mycoplasma respiratory infections.", "content": "Intranasal inoculation of M. pulmonis in mice and M. pneumoniae in hamsters results in pneumonia characterised by peribronchiolar and perivascular cuffing by lymphocytes. Thymus-dependent lymphocytes were depleted in mice by thymectomy and X-irradiation or treatment with anti-lymphocyte serum (ALS), and in hamsters by treatment with ALS. These procedures caused a reduction in the severity of pneumonic lesions in infected animals compared with infected immunologically normal animals. In addition, the organisms were present in slightly greater numbers in the lungs of the immunosuppressed animals. These results indicate the importance of thymus-dependent lymphocytes in the pathogenesis of mycoplasma-induced pulmonary disease. However, the role that these cells play in resistance to infection is not known and it may be that local secretory antibody is also important. Results of preliminary experiments involving hamster tracheal organ cultures infected with M. pneumoniae indicate that there is a factor present in lung washings from immune hamsters that protects against loss of ciliary activity brought about by M. pneumoniae.", "contents": "The part played by cell-mediated immunity in mycoplasma respiratory infections. Intranasal inoculation of M. pulmonis in mice and M. pneumoniae in hamsters results in pneumonia characterised by peribronchiolar and perivascular cuffing by lymphocytes. Thymus-dependent lymphocytes were depleted in mice by thymectomy and X-irradiation or treatment with anti-lymphocyte serum (ALS), and in hamsters by treatment with ALS. These procedures caused a reduction in the severity of pneumonic lesions in infected animals compared with infected immunologically normal animals. In addition, the organisms were present in slightly greater numbers in the lungs of the immunosuppressed animals. These results indicate the importance of thymus-dependent lymphocytes in the pathogenesis of mycoplasma-induced pulmonary disease. However, the role that these cells play in resistance to infection is not known and it may be that local secretory antibody is also important. Results of preliminary experiments involving hamster tracheal organ cultures infected with M. pneumoniae indicate that there is a factor present in lung washings from immune hamsters that protects against loss of ciliary activity brought about by M. pneumoniae."}
{"id": "PMID:1079194", "title": "Hemobilia following percutaneous liver biopsy.", "content": "A patient underwent a routine percutaneous liver biopsy and subsequently developed gastrointestinal hemorrhage, biliary colic, and bilirubinuria suggesting the presence of hemobilia. After a negative arteriogram endoscopic cholangiography was used to confirm the diagnosis.", "contents": "Hemobilia following percutaneous liver biopsy. A patient underwent a routine percutaneous liver biopsy and subsequently developed gastrointestinal hemorrhage, biliary colic, and bilirubinuria suggesting the presence of hemobilia. After a negative arteriogram endoscopic cholangiography was used to confirm the diagnosis."}
{"id": "PMID:1079195", "title": "Hypersensitivity reactions in small intestine. I Thymus dependence of experimental 'partial villous atrophy'.", "content": "Rats infected with the intestinal nematode Nippostrongylus brasiliensis have crypt hyperplasia with villous atrophy in affected areas of the small intestine. In thymus-deprived (B) rats the course of infection is prolonged but, despite the presence of many worms in the intestinal lumen, villi and crypts appear largely normal. This suggests that the tissue damaged associated with N. brasilliensis infection is caused, not by the worms, but by a local thymus-dependent immune reaction. There is some evidence to implicate lymphocytes rather than antibodies in this reaction. It is already know that T-cell-associated damage to the small intestine, such as occurs in allograft rejection, produces subtotal villous atrophy. The present findings suggest that when T cell react locally with helminth antigens a similar type of damage occurs. The presence of a local cell-mediated immune reaction may be the common factor which causes villous atrophy and crypt hyperplasia in many small intestinal diseases, eg, viral enteritis, giardiasis, cow's milk allergy, and coeliac disease.", "contents": "Hypersensitivity reactions in small intestine. I Thymus dependence of experimental 'partial villous atrophy'. Rats infected with the intestinal nematode Nippostrongylus brasiliensis have crypt hyperplasia with villous atrophy in affected areas of the small intestine. In thymus-deprived (B) rats the course of infection is prolonged but, despite the presence of many worms in the intestinal lumen, villi and crypts appear largely normal. This suggests that the tissue damaged associated with N. brasilliensis infection is caused, not by the worms, but by a local thymus-dependent immune reaction. There is some evidence to implicate lymphocytes rather than antibodies in this reaction. It is already know that T-cell-associated damage to the small intestine, such as occurs in allograft rejection, produces subtotal villous atrophy. The present findings suggest that when T cell react locally with helminth antigens a similar type of damage occurs. The presence of a local cell-mediated immune reaction may be the common factor which causes villous atrophy and crypt hyperplasia in many small intestinal diseases, eg, viral enteritis, giardiasis, cow's milk allergy, and coeliac disease."}
{"id": "PMID:1079196", "title": "A British family with herediatary pancreatitis.", "content": "A family with hereditary pancreatitis is described. Nine family members definitely have had pancreatitis, whilst 15 more are suspected of having the disease. The condition presents as recurrent attacks of epigastric or central abdominal pain, sometimes radiating to the back, often associated with vomiting. The attacks of pain usually last three to four days. The inheritance fits well with an autosomal dominant pattern with limited penetrance, as it does in other families described in the literature. There is no aminoaciduria as has been described in some previously reported families. The attacks of pain start in childhood or young adult life (mean age of onset inthis family is 12-6 years) and appear to cease in this family by the age of 40 years. The diagnosis of pancreatitis in members of the family who have had confirmed pancreatitis was made by finding a raised serum amylase concentration in four cases, at laparotomy in four cases, and by pancreatic calcification seen on radiography in one case, The literature on the condition is reviewed, and it is speculated that the condition may have been underdiagnosed in Britain.", "contents": "A British family with herediatary pancreatitis. A family with hereditary pancreatitis is described. Nine family members definitely have had pancreatitis, whilst 15 more are suspected of having the disease. The condition presents as recurrent attacks of epigastric or central abdominal pain, sometimes radiating to the back, often associated with vomiting. The attacks of pain usually last three to four days. The inheritance fits well with an autosomal dominant pattern with limited penetrance, as it does in other families described in the literature. There is no aminoaciduria as has been described in some previously reported families. The attacks of pain start in childhood or young adult life (mean age of onset inthis family is 12-6 years) and appear to cease in this family by the age of 40 years. The diagnosis of pancreatitis in members of the family who have had confirmed pancreatitis was made by finding a raised serum amylase concentration in four cases, at laparotomy in four cases, and by pancreatic calcification seen on radiography in one case, The literature on the condition is reviewed, and it is speculated that the condition may have been underdiagnosed in Britain."}
{"id": "PMID:1079197", "title": "The immune response of mice to phiX174. The potentiation of B-cell immunity and the suppression of T-cell help by pertussis vaccine.", "content": "The primary and secondary responses to phiX174 have been studied in T cell-deprived (T- minus) and control CBA mice including sham-operated (T+) and normal mice. The effect of pertussis on the immune response of these mice has been measured. It is concluded that: (1) the gammaG and memory responses to phiX are produced in T- minus mice even though greatly reduced in comparison with T+ and normal mice; (2) pertussis appears to enhance the gammaG response in T- minus mice while suppressing it in T+ and normal mice.", "contents": "The immune response of mice to phiX174. The potentiation of B-cell immunity and the suppression of T-cell help by pertussis vaccine. The primary and secondary responses to phiX174 have been studied in T cell-deprived (T- minus) and control CBA mice including sham-operated (T+) and normal mice. The effect of pertussis on the immune response of these mice has been measured. It is concluded that: (1) the gammaG and memory responses to phiX are produced in T- minus mice even though greatly reduced in comparison with T+ and normal mice; (2) pertussis appears to enhance the gammaG response in T- minus mice while suppressing it in T+ and normal mice."}
{"id": "PMID:1079199", "title": "Permanent hapten-specific tolerance in B lymphocytes.", "content": "Tolerance to the hapten TNP was induced in mice congenic with CBA but bearing the Ig-b allotype (Ig-b mice). To induce a high degree of tolerance it was necessary to give five injections of TNP-sulphonate followed by an immunogenic challenge (alum precipitate of TNP-BSA with pertussis adjuvant). Lymph node or spleen cells from these mice were transferred, with or without an equal number of non-tolerant CBA spleen cells, to irradiated CBA recipients and these were challenged with a different TNP-protein conjugate. Anti-TNP antibody bearing the Ig-b allotype was then assayed separately from total anti-TNP, as a measure of the contributions made by tolerant and non-tolerant B-cell populations respectively. Tolerant lymph node cell did not depress the response of normal cells, nor did the normal cells 'break' the tolerance of the Ig-b population even when the latter had been treated with anti-T-cell serum and complement. No response was obtained from tolerant lymph node cells when the recipients were challenged at different time up to 12 weeks after transfer. By this time the control non-tolerant lymph node cells had also lost the capacity to respond. It is concluded that: (1) effectively permanent tolerance, which is not maintained by afferent mechanisms, can be induced in lymph node B cells; (2) B-cell tolerance can be greatly enhanced by immunogenic challenge; (3) spleen may contain a distinct population of B cells which is less susceptible to tolerance; and (4) the life-span of virgin lymph node B cells is probably less than 12 weeks.", "contents": "Permanent hapten-specific tolerance in B lymphocytes. Tolerance to the hapten TNP was induced in mice congenic with CBA but bearing the Ig-b allotype (Ig-b mice). To induce a high degree of tolerance it was necessary to give five injections of TNP-sulphonate followed by an immunogenic challenge (alum precipitate of TNP-BSA with pertussis adjuvant). Lymph node or spleen cells from these mice were transferred, with or without an equal number of non-tolerant CBA spleen cells, to irradiated CBA recipients and these were challenged with a different TNP-protein conjugate. Anti-TNP antibody bearing the Ig-b allotype was then assayed separately from total anti-TNP, as a measure of the contributions made by tolerant and non-tolerant B-cell populations respectively. Tolerant lymph node cell did not depress the response of normal cells, nor did the normal cells 'break' the tolerance of the Ig-b population even when the latter had been treated with anti-T-cell serum and complement. No response was obtained from tolerant lymph node cells when the recipients were challenged at different time up to 12 weeks after transfer. By this time the control non-tolerant lymph node cells had also lost the capacity to respond. It is concluded that: (1) effectively permanent tolerance, which is not maintained by afferent mechanisms, can be induced in lymph node B cells; (2) B-cell tolerance can be greatly enhanced by immunogenic challenge; (3) spleen may contain a distinct population of B cells which is less susceptible to tolerance; and (4) the life-span of virgin lymph node B cells is probably less than 12 weeks."}
{"id": "PMID:1079200", "title": "Subpopulations of mouse spleen lymphocytes. II. Immunological reactivity of spleen cells fractionated on BSA density gradients.", "content": "We found in previous experiments that fractionation of non-immune mouse spleen cells on bovine serum albumin density gradients yields two subpopulations of T cells, one of high, the other of low density. Both subopopulations could be stimulated in corporate thymidine by the T cell-specific mitogen concanavalin A (con A). In the present investigation, spleen cells of mice immunized to sheep red cells (SRC) were similarly fractionated and the fractions recovered were assayed for: (a) reactivity to con A; (B) REACTIVITY TO SRC and (c) capacity to function as helper cells when stimulated with the homologous (SRC) or with a heterologous (donkey red cells) (DRC) antigen. Two subpopulations of cells reacting to con A were found in the spleens of the primed mice, corresponding to the subpopulations found in the non-immune mice. Both subpopulations contained cells responding to SRC (as measured by thymidine incorporation) and cells endowed with helper activity. The two subpopulations appeared to differ, however, in their specificity: while the denser cells could only exert their helper effect when stimulated by the specific antigen, the light cells could be effectively stimulated by both the specific (SRC) and the nonspecific (DRC) antigen.", "contents": "Subpopulations of mouse spleen lymphocytes. II. Immunological reactivity of spleen cells fractionated on BSA density gradients. We found in previous experiments that fractionation of non-immune mouse spleen cells on bovine serum albumin density gradients yields two subpopulations of T cells, one of high, the other of low density. Both subopopulations could be stimulated in corporate thymidine by the T cell-specific mitogen concanavalin A (con A). In the present investigation, spleen cells of mice immunized to sheep red cells (SRC) were similarly fractionated and the fractions recovered were assayed for: (a) reactivity to con A; (B) REACTIVITY TO SRC and (c) capacity to function as helper cells when stimulated with the homologous (SRC) or with a heterologous (donkey red cells) (DRC) antigen. Two subpopulations of cells reacting to con A were found in the spleens of the primed mice, corresponding to the subpopulations found in the non-immune mice. Both subpopulations contained cells responding to SRC (as measured by thymidine incorporation) and cells endowed with helper activity. The two subpopulations appeared to differ, however, in their specificity: while the denser cells could only exert their helper effect when stimulated by the specific antigen, the light cells could be effectively stimulated by both the specific (SRC) and the nonspecific (DRC) antigen."}
{"id": "PMID:1079201", "title": "Serologic evidence for cross-reacting antigens in two carcinogen-induced murine sarcomas.", "content": "Serological evidence is presented that two chemically-induced (by methylcholanthrene and 3,4-benz(a)pyrene) sarcomas of C3HF mice contain cross-reacting tumor-associated cell-surface antigens. Xenogeneic and syngeneic antitumor antisera against the two sarcomas were studied with an isotopic, complement-dependent, antibody-mediated microcytotoxicity assay and an isotopic antiglobulin test for the detection of antibodies to tumor-associated antigens, in vitro. Absorportions with various tissues were performed which consistently revealed that the specific activity of the antitumor antisera could be removed by absorption with cells from either chemically-induced tumor, while absorption with syngeneic normal adult tissues, normal fetal tissues, or cells from a histogenetically unrelated tumor (spontaneous mammary carcinoma) failed to remove any specific activity. In view of the individual character of carcinogen-induced tumor antigens as detected by tumor transplantation techniques, our results suggest that chemically-induced murine sarcomas (even when induced by different carcinogens) contain both private and common cell-surface antigens, the latter detectable by serological methods. These common tumor-associated antigens may be related to a viral genome involved in the nalignant transformation of carcinogen-induced marine sarcomas.", "contents": "Serologic evidence for cross-reacting antigens in two carcinogen-induced murine sarcomas. Serological evidence is presented that two chemically-induced (by methylcholanthrene and 3,4-benz(a)pyrene) sarcomas of C3HF mice contain cross-reacting tumor-associated cell-surface antigens. Xenogeneic and syngeneic antitumor antisera against the two sarcomas were studied with an isotopic, complement-dependent, antibody-mediated microcytotoxicity assay and an isotopic antiglobulin test for the detection of antibodies to tumor-associated antigens, in vitro. Absorportions with various tissues were performed which consistently revealed that the specific activity of the antitumor antisera could be removed by absorption with cells from either chemically-induced tumor, while absorption with syngeneic normal adult tissues, normal fetal tissues, or cells from a histogenetically unrelated tumor (spontaneous mammary carcinoma) failed to remove any specific activity. In view of the individual character of carcinogen-induced tumor antigens as detected by tumor transplantation techniques, our results suggest that chemically-induced murine sarcomas (even when induced by different carcinogens) contain both private and common cell-surface antigens, the latter detectable by serological methods. These common tumor-associated antigens may be related to a viral genome involved in the nalignant transformation of carcinogen-induced marine sarcomas."}
{"id": "PMID:1079202", "title": "In vitro immunological studies on East African cancer patients. II. Increased sensitivity of blood lymphocytes from untreated Burkitt lymphoma patients to inhibition of spontaneous rosette formation.", "content": "Frozen aliquots of peripheral blood mononuclear cells from patients with Burkitt's lymphoma (BL) at various stages of the disease have been compared with samples from East African children who were healthy or who suffered from non-malinant disorders. Using the sheep-cell rosette test (which is believed to identify T-lymphocytes), we found a significant reduction in the proportion of rosette-forming cells (RFC) in the samples from untreated BL patients. After reduction of remission the difference between BL patients and controls was abolished. Rosette formation was more readily inhibited by anti-lymphocyte globulin (ALG) in samples from untreated BL patients than in controls. On induction of remission the rosette inhibition curve shifted towards the control values. The findings are compatible with the presence, in untreated BL, of a circulating factor which impairs the capacity of T-lymphocytes to form rosettes with sheep erythrocytes. It remains to be established whether the functional capacity of T-cells to protect the host against tumour proliferation is also impaired.", "contents": "In vitro immunological studies on East African cancer patients. II. Increased sensitivity of blood lymphocytes from untreated Burkitt lymphoma patients to inhibition of spontaneous rosette formation. Frozen aliquots of peripheral blood mononuclear cells from patients with Burkitt's lymphoma (BL) at various stages of the disease have been compared with samples from East African children who were healthy or who suffered from non-malinant disorders. Using the sheep-cell rosette test (which is believed to identify T-lymphocytes), we found a significant reduction in the proportion of rosette-forming cells (RFC) in the samples from untreated BL patients. After reduction of remission the difference between BL patients and controls was abolished. Rosette formation was more readily inhibited by anti-lymphocyte globulin (ALG) in samples from untreated BL patients than in controls. On induction of remission the rosette inhibition curve shifted towards the control values. The findings are compatible with the presence, in untreated BL, of a circulating factor which impairs the capacity of T-lymphocytes to form rosettes with sheep erythrocytes. It remains to be established whether the functional capacity of T-cells to protect the host against tumour proliferation is also impaired."}
{"id": "PMID:1079203", "title": "In vitro immunological studies on east african cancer patients. III. Spontaneous rosette formation by cells from Burkitt lymphoma biopsies.", "content": "Spontaneous rosette formation was observed in eight out of nine Burkitt lymphoma biopsies. These were examined fresh and/or after culture in vitro for up to 48 h. The percentage of rosettes varied from 3.7% to 38%. There was a tendency for the percentage of rosettes to increase with time in tissue culture. It is suggested that the rosette-forming cells are T-lymphocytes infiltrating the tumour and functioning as a host defence mechanism against the proliferating tumour cells.", "contents": "In vitro immunological studies on east african cancer patients. III. Spontaneous rosette formation by cells from Burkitt lymphoma biopsies. Spontaneous rosette formation was observed in eight out of nine Burkitt lymphoma biopsies. These were examined fresh and/or after culture in vitro for up to 48 h. The percentage of rosettes varied from 3.7% to 38%. There was a tendency for the percentage of rosettes to increase with time in tissue culture. It is suggested that the rosette-forming cells are T-lymphocytes infiltrating the tumour and functioning as a host defence mechanism against the proliferating tumour cells."}
{"id": "PMID:1079204", "title": "Immunological mechanisms in the pathogenesis of virus-induced murine leukemia. I. Autoreactivity.", "content": "By an in vitro microcytotoxicity assay, thymocytes from mice infected with Moloney murine leukemia virus since birth (MuLV-M carriers) caused a dramatic reduction of normal, non-infected syngeneic target cells; they usually spared identically derived target cells infected with MuLV-M. Thymocytes from normal mice slightly stimulated the replication of the same target cells. This pattern of carrier thymocyte reactivity was evident at all ages tested during both preneoplastic and neoplastic periods. Transplantable lymphoma cells derived from a carrier mouse with overt thymic lymphoma behaved similarly. Lymphocytes from the spleens and lymph nodes of young preleukemic carriers (8-12 weeks old) usually were reactive against MuLV-M-infected tarted cells and not against non-infected target cells. In contrast, lymphocytes from older preleukemic carriers (16-17 weeks old) reacted in a manner similar to carrier thymocytes. These observations suggest that the thymic lymphoma induced by MuLV-M may result in part from an autoreactive process originating in the thymus, but eventually spreading to the peripheral lymphoid tissues.", "contents": "Immunological mechanisms in the pathogenesis of virus-induced murine leukemia. I. Autoreactivity. By an in vitro microcytotoxicity assay, thymocytes from mice infected with Moloney murine leukemia virus since birth (MuLV-M carriers) caused a dramatic reduction of normal, non-infected syngeneic target cells; they usually spared identically derived target cells infected with MuLV-M. Thymocytes from normal mice slightly stimulated the replication of the same target cells. This pattern of carrier thymocyte reactivity was evident at all ages tested during both preneoplastic and neoplastic periods. Transplantable lymphoma cells derived from a carrier mouse with overt thymic lymphoma behaved similarly. Lymphocytes from the spleens and lymph nodes of young preleukemic carriers (8-12 weeks old) usually were reactive against MuLV-M-infected tarted cells and not against non-infected target cells. In contrast, lymphocytes from older preleukemic carriers (16-17 weeks old) reacted in a manner similar to carrier thymocytes. These observations suggest that the thymic lymphoma induced by MuLV-M may result in part from an autoreactive process originating in the thymus, but eventually spreading to the peripheral lymphoid tissues."}
{"id": "PMID:1079205", "title": "The antigenicity of carcinoembryonic antigen in man.", "content": "Binding of globulins to -125I CEA was detected in 10 of 24 sera from patients with cancer, 13 of 26 sera from blood donors, and 7 of 27 supernates of tumor-cell suspensions. Binding was predominantly by IgM in 10 of 14 sera tested by radioimmunoelectrophoresis. Binding was inhibited by 6 ng unlabelled CEA in one instance only, and by adsorption with blood group A erythrocytes in three cases. Binding was not increased in dialysed precipitates formed by ammonium sulfate at pH 2.5, while there was increased binding in precipitates of complexes of CEA and heterologous anti-CEA. These results confirm that human IgM binds to CEA, and in some cases binding appears to be mediated by antibodies to blood group A. However, the binding is weak and appears to be of low affinity. This suggests that binding of CEA by immunoglobulins represents cross-reactivity by antibodies to similar antigens, of which blood group A is one example.", "contents": "The antigenicity of carcinoembryonic antigen in man. Binding of globulins to -125I CEA was detected in 10 of 24 sera from patients with cancer, 13 of 26 sera from blood donors, and 7 of 27 supernates of tumor-cell suspensions. Binding was predominantly by IgM in 10 of 14 sera tested by radioimmunoelectrophoresis. Binding was inhibited by 6 ng unlabelled CEA in one instance only, and by adsorption with blood group A erythrocytes in three cases. Binding was not increased in dialysed precipitates formed by ammonium sulfate at pH 2.5, while there was increased binding in precipitates of complexes of CEA and heterologous anti-CEA. These results confirm that human IgM binds to CEA, and in some cases binding appears to be mediated by antibodies to blood group A. However, the binding is weak and appears to be of low affinity. This suggests that binding of CEA by immunoglobulins represents cross-reactivity by antibodies to similar antigens, of which blood group A is one example."}
{"id": "PMID:1079206", "title": "Stimulation of Fracture Healing by Pacemaker in Rabbits.201S.", "content": "An und-splaced fracture of each fibula was produced in 20 rabbits. Into the fracture of the right fibula, a cathode was inserted, which delivered 15 microamperes of galvanic current for more than one week. The source of current was 5v small transistor batteries which acted like a pacemaker. This stimulated healing of the fracture for 15 days. The left fibula fractures were used as controls. Both fibulae were studied roentgenographically and microscopically. This experiment suggested that galvanic current with a cathode placed in the fracture gap stimulated fracture healing.", "contents": "Stimulation of Fracture Healing by Pacemaker in Rabbits.201S. An und-splaced fracture of each fibula was produced in 20 rabbits. Into the fracture of the right fibula, a cathode was inserted, which delivered 15 microamperes of galvanic current for more than one week. The source of current was 5v small transistor batteries which acted like a pacemaker. This stimulated healing of the fracture for 15 days. The left fibula fractures were used as controls. Both fibulae were studied roentgenographically and microscopically. This experiment suggested that galvanic current with a cathode placed in the fracture gap stimulated fracture healing."}
{"id": "PMID:1079207", "title": "Familial occurrence of dot (microcystic), map, fingerprint dystrophy of the cornea.", "content": "The clinical description of corneal microcysts, maplike changes, and fingerprints has led investigators to the conclusion that these changes may represent a corneal dystrophy. The familial or hereditary evidence which is usually necessary to label a corneal disease a dystrophy has been lacking. This paper describes a familial pattern of disease in two families where three generations were involved and in eight families with corneal changes in at least two generations.", "contents": "Familial occurrence of dot (microcystic), map, fingerprint dystrophy of the cornea. The clinical description of corneal microcysts, maplike changes, and fingerprints has led investigators to the conclusion that these changes may represent a corneal dystrophy. The familial or hereditary evidence which is usually necessary to label a corneal disease a dystrophy has been lacking. This paper describes a familial pattern of disease in two families where three generations were involved and in eight families with corneal changes in at least two generations."}
{"id": "PMID:1079208", "title": "[Lymphoreticular proliferations in the skin. Cytochemical and immunocytological studies in lymphadenosis benigna cutis].", "content": "In 8 patients with lymphadenosis benigna cutis (LABC) cytochemical and in 2 of them immunocytological studies have been performed. 1) In patients with LABC we find ectopic organoid proliferations of \"lymphfollicle\"-like structures within the dermis which predominately consist of small lymphocytes and large reticulum cells. Immunocytological differentiation of the lymphocytes leads to the characterization of B- and T-lymphocytes in a ration 2:1. 2) Large reticulum cells represent a peculiarly remarkable cell class in infiltrates of LABC. Because of their typical arrangement disseminated within the lymphocytic infiltrate they have been designated as \"starry sky\" cells. Cytochemically they are characterized by an unusual high content of nonspecific esterases and acid phosphatase, most of them show phagocytized basophilic bodies. Because of their shape, arrangement and enzymcytochemical behaviour these cells can be referred to as typical for the LABC disease. 3) Monocytes cannot be found within the \"lymphfollicles\". Mast cells and connective tissue cells are rarely observed. Polymorphonuclear granulocytes can be demonstrated in great numbers in any part of the involved cutis when there is an insect bite in history. 4) As a reaction of the ectopic proliferation of lymphoreticular tissue within the dermis there is an activation of the surrounding connective tissue with an increase of the alkaline phosphatase activity within these cells, new formation of collagen fibres and strong proliferation of alkaline phosphatase positive capillaries. 5) Etiopathologically it is stressed, that in LABC for example an insect bite induces stimulation of hematopoietic potentialities of undifferentiated mesenchymal germ centres within the cutis takes place, leading to the development of ectopic of \"lymphfollicle\" like structures.", "contents": "[Lymphoreticular proliferations in the skin. Cytochemical and immunocytological studies in lymphadenosis benigna cutis]. In 8 patients with lymphadenosis benigna cutis (LABC) cytochemical and in 2 of them immunocytological studies have been performed. 1) In patients with LABC we find ectopic organoid proliferations of \"lymphfollicle\"-like structures within the dermis which predominately consist of small lymphocytes and large reticulum cells. Immunocytological differentiation of the lymphocytes leads to the characterization of B- and T-lymphocytes in a ration 2:1. 2) Large reticulum cells represent a peculiarly remarkable cell class in infiltrates of LABC. Because of their typical arrangement disseminated within the lymphocytic infiltrate they have been designated as \"starry sky\" cells. Cytochemically they are characterized by an unusual high content of nonspecific esterases and acid phosphatase, most of them show phagocytized basophilic bodies. Because of their shape, arrangement and enzymcytochemical behaviour these cells can be referred to as typical for the LABC disease. 3) Monocytes cannot be found within the \"lymphfollicles\". Mast cells and connective tissue cells are rarely observed. Polymorphonuclear granulocytes can be demonstrated in great numbers in any part of the involved cutis when there is an insect bite in history. 4) As a reaction of the ectopic proliferation of lymphoreticular tissue within the dermis there is an activation of the surrounding connective tissue with an increase of the alkaline phosphatase activity within these cells, new formation of collagen fibres and strong proliferation of alkaline phosphatase positive capillaries. 5) Etiopathologically it is stressed, that in LABC for example an insect bite induces stimulation of hematopoietic potentialities of undifferentiated mesenchymal germ centres within the cutis takes place, leading to the development of ectopic of \"lymphfollicle\" like structures."}
{"id": "PMID:1079209", "title": "Viridenomycin, a new antibiotic.", "content": "Viridenomycin, a new crystalline antibiotic, was isolated from the culture broth of Streptomyces viridochromogenes strain No. T-24146. Viridenomycin is a weakly acidic and lipophilic substance, which exhibits a strong absorption maximum at 310 nm and shows strong activity against Trichomonas vaginalis and gram-positive bacteria.", "contents": "Viridenomycin, a new antibiotic. Viridenomycin, a new crystalline antibiotic, was isolated from the culture broth of Streptomyces viridochromogenes strain No. T-24146. Viridenomycin is a weakly acidic and lipophilic substance, which exhibits a strong absorption maximum at 310 nm and shows strong activity against Trichomonas vaginalis and gram-positive bacteria."}
{"id": "PMID:1079211", "title": "Alpha-1-antitrypsin content in the serum, alveolar macrophages, and alveolar lavage fluid of smoking and nonsmoking normal subjects.", "content": "The content of alpha-1-antitrypsin in the serum, alveolar lavage fluid, and alveolar macrophages of smokers and nonsmokers was studied. Bronchoalveolar lavage was used to obtain alveolar fluid and macrophages from normal volunteers, and alpha-1-antitrypsin and albumin were measured using the electroimmunodiffusion technique. The serum level of inhibitor was not different between the two groups, while the total lavage fliud content of alpha-1-antitrypsin was increased in the smokers. The level of alpha-1-antitrypsin was also significantly greater (P less than 0.001) in the alveolar macrophages of the smokers suggesting the possibility of chronically increased alveolar levels in the cigarette smoker as a possible protective mechanism against proteolysis.", "contents": "Alpha-1-antitrypsin content in the serum, alveolar macrophages, and alveolar lavage fluid of smoking and nonsmoking normal subjects. The content of alpha-1-antitrypsin in the serum, alveolar lavage fluid, and alveolar macrophages of smokers and nonsmokers was studied. Bronchoalveolar lavage was used to obtain alveolar fluid and macrophages from normal volunteers, and alpha-1-antitrypsin and albumin were measured using the electroimmunodiffusion technique. The serum level of inhibitor was not different between the two groups, while the total lavage fliud content of alpha-1-antitrypsin was increased in the smokers. The level of alpha-1-antitrypsin was also significantly greater (P less than 0.001) in the alveolar macrophages of the smokers suggesting the possibility of chronically increased alveolar levels in the cigarette smoker as a possible protective mechanism against proteolysis."}
{"id": "PMID:1079212", "title": "Scientific methodology applied.", "content": "The subject of this symposium is naproxen, a new drug that resulted from an investigation to find a superior anti-inflammatory agent. It was synthesized by Harrison et al. in 1970 at the Syntex Institute of Organic Chemistry and Biological Sciences. How can we chart the evolution of this or any other drug? Three steps are necessary: first, chemical studies (synthesis, analysis); second, animal pharmacology; third, human pharmacology. The last step can additionally be divided into four phases: metabolism and toxicology of the drug in normal volunteers; dose titration and initial clinical trials with sick subjects (pharmacometry); confirmatory clinical trials when the drug is accepted on the market and revaluation (familiarization trials). To discover the truth about naproxen, we must all participate actively with a critical mind, following the principles of scientific methodology. We shall find that the papers to be presented today all deal with the third step in the evaluation process--clinical pharmacology. It is quite evident that the final and most decisive test must be aimed at the most valuable target: the human being. The end product of this day's work for each of us should be the formation of an opinion based on solid scientific proofs. And let us hope that we will all enjoy fulfilling the symposium in its entire etymological meaning this evening. In vino veritas.", "contents": "Scientific methodology applied. The subject of this symposium is naproxen, a new drug that resulted from an investigation to find a superior anti-inflammatory agent. It was synthesized by Harrison et al. in 1970 at the Syntex Institute of Organic Chemistry and Biological Sciences. How can we chart the evolution of this or any other drug? Three steps are necessary: first, chemical studies (synthesis, analysis); second, animal pharmacology; third, human pharmacology. The last step can additionally be divided into four phases: metabolism and toxicology of the drug in normal volunteers; dose titration and initial clinical trials with sick subjects (pharmacometry); confirmatory clinical trials when the drug is accepted on the market and revaluation (familiarization trials). To discover the truth about naproxen, we must all participate actively with a critical mind, following the principles of scientific methodology. We shall find that the papers to be presented today all deal with the third step in the evaluation process--clinical pharmacology. It is quite evident that the final and most decisive test must be aimed at the most valuable target: the human being. The end product of this day's work for each of us should be the formation of an opinion based on solid scientific proofs. And let us hope that we will all enjoy fulfilling the symposium in its entire etymological meaning this evening. In vino veritas."}
{"id": "PMID:1079213", "title": "Effect of naproxen on gastrointestinal microbleeding following acetylsalicylate medication.", "content": "This study was undertaken to determine if substitution of naproxen for ASA does influence salicylate-induced gastrointestinal bleeding. Twelve normal volunteers were selected and given increasing doses of salicylate until guaiac tests were consistently positive. Autologous labeling of their red blood cells with 51-Cr was used to quantitate the microbleeding. After two weeks on ASA, six subjects were double blindly switched to naproxen and six to placebo for another two weeks of observation. Two-way analysis of variance on the raw data shows a significant treatment effect associated with a significant interaction in both groups. Final analysis on a logarithmic scale permits orthogonal contrasts to be accurately made without any significant remaining interaction. It is concluded that substitution of naproxen for ASA at a dose of 500 mg daily is accompanied by a rapid reduction of microbleeding to \"normal\" levels.", "contents": "Effect of naproxen on gastrointestinal microbleeding following acetylsalicylate medication. This study was undertaken to determine if substitution of naproxen for ASA does influence salicylate-induced gastrointestinal bleeding. Twelve normal volunteers were selected and given increasing doses of salicylate until guaiac tests were consistently positive. Autologous labeling of their red blood cells with 51-Cr was used to quantitate the microbleeding. After two weeks on ASA, six subjects were double blindly switched to naproxen and six to placebo for another two weeks of observation. Two-way analysis of variance on the raw data shows a significant treatment effect associated with a significant interaction in both groups. Final analysis on a logarithmic scale permits orthogonal contrasts to be accurately made without any significant remaining interaction. It is concluded that substitution of naproxen for ASA at a dose of 500 mg daily is accompanied by a rapid reduction of microbleeding to \"normal\" levels."}
{"id": "PMID:1079214", "title": "Ascending afferents to the telencephalon of ranid frogs: an anterograde degeneration study.", "content": "Sources of telencephalic afferents were examined in two species of frogs, Rana catesbeiana and Rana pipiens, by study of anterograde degeneration resulting from hemisection of the brain at the isthmus or at the caudal border of the thalamus and from electrolytic lesions in various portions of the diencephalon. The results indicate that some telencephalic afferents arise from levels caudal to the isthmus, that some striatal afferents arise from a level between the isthmus and the caudal border of the thalamus, and that a projection to the ipsilateral striatum arises from the anterior and/or middle portions of the dorsal thalamus. Diencephalic projections to pallial portions of the hemisphere were also observed. These results demonstrate substantial non-olfactory afferent projections to the telencephalon in ranid frogs.", "contents": "Ascending afferents to the telencephalon of ranid frogs: an anterograde degeneration study. Sources of telencephalic afferents were examined in two species of frogs, Rana catesbeiana and Rana pipiens, by study of anterograde degeneration resulting from hemisection of the brain at the isthmus or at the caudal border of the thalamus and from electrolytic lesions in various portions of the diencephalon. The results indicate that some telencephalic afferents arise from levels caudal to the isthmus, that some striatal afferents arise from a level between the isthmus and the caudal border of the thalamus, and that a projection to the ipsilateral striatum arises from the anterior and/or middle portions of the dorsal thalamus. Diencephalic projections to pallial portions of the hemisphere were also observed. These results demonstrate substantial non-olfactory afferent projections to the telencephalon in ranid frogs."}
{"id": "PMID:1079215", "title": "Binding of poly (Glu-60 Ala-30 Tyr-10) by thymic lymphocytes from genetic responder and non-responder mice: effect of antihistocompatibility serum.", "content": "The binding of 125-I-labeled GAT by murine thymocytes was studied by autoradiography. GAT binding was highly temperature dependent. Significantly more thymocytes bound GAT at 37 degrees C than at 4 degrees C. GAT bound at 37 degrees C was usually distributed in a \"cap\" over one pole of the cell. Preincubation of thymocytes with specific anti-H-2 serum markedly inhibited the subsequent binding of GAT. The frequency of antigen-binding thymocytes was not significantly affected by preincubation of thymocytes with anti-Thy-1 serum or a number of anti-immunoglobulin sera. The immune response to GAT is controlled by an H-2 linked immune response gene(s). Mice of strains that are H-2p, H-2q or H-2s (non-responders) fail to develop effective T cell helper function necessary for a humoral immune response to GAT under most conditions. However, both responder and non-responder strains had similar numbers of thymocytes capable of binding GAT in vitro, indicating that T cells from non-responder strains of mice do possess a recognition mechanism for GAT.", "contents": "Binding of poly (Glu-60 Ala-30 Tyr-10) by thymic lymphocytes from genetic responder and non-responder mice: effect of antihistocompatibility serum. The binding of 125-I-labeled GAT by murine thymocytes was studied by autoradiography. GAT binding was highly temperature dependent. Significantly more thymocytes bound GAT at 37 degrees C than at 4 degrees C. GAT bound at 37 degrees C was usually distributed in a \"cap\" over one pole of the cell. Preincubation of thymocytes with specific anti-H-2 serum markedly inhibited the subsequent binding of GAT. The frequency of antigen-binding thymocytes was not significantly affected by preincubation of thymocytes with anti-Thy-1 serum or a number of anti-immunoglobulin sera. The immune response to GAT is controlled by an H-2 linked immune response gene(s). Mice of strains that are H-2p, H-2q or H-2s (non-responders) fail to develop effective T cell helper function necessary for a humoral immune response to GAT under most conditions. However, both responder and non-responder strains had similar numbers of thymocytes capable of binding GAT in vitro, indicating that T cells from non-responder strains of mice do possess a recognition mechanism for GAT."}
{"id": "PMID:1079216", "title": "Migratory patterns of B lymphocytes. III. Inhibition of splenic follicular localization of transferred chicken bursa cells by preincubation with anti-Ig-1.", "content": "Homing of 3-H-adenosine-labeled bursa cells to follicles in the chickens spleen was greatly reduced by prior incubation of the transferred cells with rabbit anti-chicken-immunoglobulin sera. This effect was transient, being present 6 hr after cell transfer but gone by 18 hr, when incubation was at 0 degreesC without complement. The inhibition persisted until at least 18 hr after incubation at 37 degrees C with complement.", "contents": "Migratory patterns of B lymphocytes. III. Inhibition of splenic follicular localization of transferred chicken bursa cells by preincubation with anti-Ig-1. Homing of 3-H-adenosine-labeled bursa cells to follicles in the chickens spleen was greatly reduced by prior incubation of the transferred cells with rabbit anti-chicken-immunoglobulin sera. This effect was transient, being present 6 hr after cell transfer but gone by 18 hr, when incubation was at 0 degreesC without complement. The inhibition persisted until at least 18 hr after incubation at 37 degrees C with complement."}
{"id": "PMID:1079217", "title": "Cell interactions between histoincompatible T and B lymphocytes. VIII. In vivo cooperative responses between lymphocytes are controlled by genes in the K-end of the H-2 complex.", "content": "The genetic restrictions for successful carrier-primed T cell and hapten-primed B cell cooperative interactions were established in a totally in vivo double adoptive transfer system. In complementation experiments, we have demonstrated that genes localized in the K-end of the H-2 complex control successful T-B cell cooperative interactions. Thus, DBA/2 KLH-primed T cells cooperated with (C3H times C3H.OH)F1 and (C3H times C3H.OL)F1 DNP-primed B cells to make a secondary response to DNP-KLH, but not with (C3H times C3H.A)F1 DNP-PRImed cells. The presence of foreign histocompatibility determinants on the F1 B cells did not prevent successful physiologic cell cooperation provided the T and B cells shared common cell interaction genes coded for in the K-end of the H-2 complex.", "contents": "Cell interactions between histoincompatible T and B lymphocytes. VIII. In vivo cooperative responses between lymphocytes are controlled by genes in the K-end of the H-2 complex. The genetic restrictions for successful carrier-primed T cell and hapten-primed B cell cooperative interactions were established in a totally in vivo double adoptive transfer system. In complementation experiments, we have demonstrated that genes localized in the K-end of the H-2 complex control successful T-B cell cooperative interactions. Thus, DBA/2 KLH-primed T cells cooperated with (C3H times C3H.OH)F1 and (C3H times C3H.OL)F1 DNP-primed B cells to make a secondary response to DNP-KLH, but not with (C3H times C3H.A)F1 DNP-PRImed cells. The presence of foreign histocompatibility determinants on the F1 B cells did not prevent successful physiologic cell cooperation provided the T and B cells shared common cell interaction genes coded for in the K-end of the H-2 complex."}
{"id": "PMID:1079218", "title": "Failure of pentamidine isethionate to provide chemoprophylaxis against Pneumocytis carinii infection in rats.", "content": "Latent Pneumocystis carinii infections were exacerbated in 54 adult male Sprague-Dawley rats by administration of alternating subcutaneous doses of hydrocortisone-21-phosphate and of cortisone acetate three times a week, plus 0.5% chlortetracycline in their drinking water, until death or the termination of the experiment at 120 days. Twenty-eight of the rats were also given pentamidine isethionate (4mg/kg) intramuscularly daily for 14 days. Pentamidine-treated animals that died within the 120 days of the experiment survived longer than control animals. At necropsy, however, every animal in both groups had P. carinii in the lungs. The intensity of the infection was the same in the pentamidine-treated and control groups. Pentamidine isethionate administered in this standard regimen does not exert a significant chemoprophylactic effect against proliferation of P. carinii in rats. The study provides little encouragement for use of pentamidine as a prophylactic agent against P. carinii in high-risk patients.", "contents": "Failure of pentamidine isethionate to provide chemoprophylaxis against Pneumocytis carinii infection in rats. Latent Pneumocystis carinii infections were exacerbated in 54 adult male Sprague-Dawley rats by administration of alternating subcutaneous doses of hydrocortisone-21-phosphate and of cortisone acetate three times a week, plus 0.5% chlortetracycline in their drinking water, until death or the termination of the experiment at 120 days. Twenty-eight of the rats were also given pentamidine isethionate (4mg/kg) intramuscularly daily for 14 days. Pentamidine-treated animals that died within the 120 days of the experiment survived longer than control animals. At necropsy, however, every animal in both groups had P. carinii in the lungs. The intensity of the infection was the same in the pentamidine-treated and control groups. Pentamidine isethionate administered in this standard regimen does not exert a significant chemoprophylactic effect against proliferation of P. carinii in rats. The study provides little encouragement for use of pentamidine as a prophylactic agent against P. carinii in high-risk patients."}
{"id": "PMID:1079219", "title": "Studies of immediate hypersensitivity in a patient with Acanthocheilonema perstans filarial infection.", "content": "A 25-year-old white female returned from West Africa with a two-year history of epidosic swelling, pruritus, and pain in a wrist, associated with peripheral eosinophilia. Serologic and immediate skin tests with Dirofilaria immitis antigen were positive, and blood smears transiently showed microfilariae of Acanthocheilonema perstans after the patient had been treated with diethylcarbamazine. Before treatment, both the serum concentration of IgE and the eosinophil content of arylsulfatase, an enzyme that selectively inactivates slow-reacting substance of anaphylaxis, were elevated; the patient's peripheral leukocytes released histamine and eosinophil chemotactic factor of anaphylaxis when challenged with D. immitis antigen. After one course of diethylcarbamazine, the clinical manifestations and abnormal in vitro immunologic results resolved. Host response to A. perstans infection appears to involve both IgE-mediated hypersensitivity and alterations in an eosinophil enzyme.", "contents": "Studies of immediate hypersensitivity in a patient with Acanthocheilonema perstans filarial infection. A 25-year-old white female returned from West Africa with a two-year history of epidosic swelling, pruritus, and pain in a wrist, associated with peripheral eosinophilia. Serologic and immediate skin tests with Dirofilaria immitis antigen were positive, and blood smears transiently showed microfilariae of Acanthocheilonema perstans after the patient had been treated with diethylcarbamazine. Before treatment, both the serum concentration of IgE and the eosinophil content of arylsulfatase, an enzyme that selectively inactivates slow-reacting substance of anaphylaxis, were elevated; the patient's peripheral leukocytes released histamine and eosinophil chemotactic factor of anaphylaxis when challenged with D. immitis antigen. After one course of diethylcarbamazine, the clinical manifestations and abnormal in vitro immunologic results resolved. Host response to A. perstans infection appears to involve both IgE-mediated hypersensitivity and alterations in an eosinophil enzyme."}
{"id": "PMID:1079222", "title": "Microsurgical approach to the cerebellopontine angle.", "content": "Various sections of the IXth, Vth, vestibular and cochlear nerves have been performed on twenty patients. The technique we used was an 'a minima' approach to the cerebellopontine angle. This operation is atraumatic and quick to perform, and the postoperative period is always excellent. It seems very useful for patients with M\u00e9ni\u00e8re's disease or intractable pains in the glossopharyngeal or trigeminal fields. It can be used for diagnosis if a small neurinoma in the cerebellopontine angle is suspected.", "contents": "Microsurgical approach to the cerebellopontine angle. Various sections of the IXth, Vth, vestibular and cochlear nerves have been performed on twenty patients. The technique we used was an 'a minima' approach to the cerebellopontine angle. This operation is atraumatic and quick to perform, and the postoperative period is always excellent. It seems very useful for patients with M\u00e9ni\u00e8re's disease or intractable pains in the glossopharyngeal or trigeminal fields. It can be used for diagnosis if a small neurinoma in the cerebellopontine angle is suspected."}
{"id": "PMID:1079233", "title": "Interactions of Fc receptors with antibodies against Ia antigens and other cell surface components.", "content": "Two Fc receptor-dependent tests were investigated to study the question of a relationship between Fc receptors and known cell surface antigens, in particular I region-associated (Ia) antigens: (a) a rosette assay with antibody-coated erythrocytes (EA) as indicator cells and normal mouse lymphoid cells as source of rosette-forming cells, and (b) a cytotoxicity test with antibody-coated erythrocytes as target cells and normal mouse spleen cells as a source of cytotoxic cells (K cells). EA rosettes were specifically inhibited by antibodies reacting with Ia antigens. Various other antisera reacting with antigens on B lymphocytes, like anti-Ly 4.2 (raised in H-2 identical mice), rabbit antimouse B-cell serum, or rabbit antimouse immunoglobulin, also specifically inhibited the rosettes. No inhibition occurred in the presence of allogeneic or xenogeneic antisera reacting with T lymphocytes. K-cell cytotoxicity was specifically inhibited by each of the antisera (reacting with either B cells or T cells). F(ab')2 fragments of anti-Ia antibodies could still specifically inhibit EA rosettes but they could not inhibit K-cell cytotoxicity. Similar results were obtained with F(ab')2 fragments of anti-immunoglobulin antibodies. These results indicate that the mechanism of inhibition of Fc receptors in the two tests was different. In neither of the tests could we find any evidence for a unique association between the Fc receptors and Ia antigens. The Fc receptors on K cells did not seem to be associated at all with Ia antigens.", "contents": "Interactions of Fc receptors with antibodies against Ia antigens and other cell surface components. Two Fc receptor-dependent tests were investigated to study the question of a relationship between Fc receptors and known cell surface antigens, in particular I region-associated (Ia) antigens: (a) a rosette assay with antibody-coated erythrocytes (EA) as indicator cells and normal mouse lymphoid cells as source of rosette-forming cells, and (b) a cytotoxicity test with antibody-coated erythrocytes as target cells and normal mouse spleen cells as a source of cytotoxic cells (K cells). EA rosettes were specifically inhibited by antibodies reacting with Ia antigens. Various other antisera reacting with antigens on B lymphocytes, like anti-Ly 4.2 (raised in H-2 identical mice), rabbit antimouse B-cell serum, or rabbit antimouse immunoglobulin, also specifically inhibited the rosettes. No inhibition occurred in the presence of allogeneic or xenogeneic antisera reacting with T lymphocytes. K-cell cytotoxicity was specifically inhibited by each of the antisera (reacting with either B cells or T cells). F(ab')2 fragments of anti-Ia antibodies could still specifically inhibit EA rosettes but they could not inhibit K-cell cytotoxicity. Similar results were obtained with F(ab')2 fragments of anti-immunoglobulin antibodies. These results indicate that the mechanism of inhibition of Fc receptors in the two tests was different. In neither of the tests could we find any evidence for a unique association between the Fc receptors and Ia antigens. The Fc receptors on K cells did not seem to be associated at all with Ia antigens."}
{"id": "PMID:1079234", "title": "Acute abdominal pain in children.", "content": "Acute abdominal pain in children is a common and challenging problem for the family physician. The many causes of this problem require a systematic approach to making the diagnosis and planning specific therapy. A careful history and physical examination, together with a small number of selected laboratory studies, provide a rational basis for effective management in most cases. This paper reviews the more common causes of acute abdominal pain in children with special emphasis on their clinical differentiation.", "contents": "Acute abdominal pain in children. Acute abdominal pain in children is a common and challenging problem for the family physician. The many causes of this problem require a systematic approach to making the diagnosis and planning specific therapy. A careful history and physical examination, together with a small number of selected laboratory studies, provide a rational basis for effective management in most cases. This paper reviews the more common causes of acute abdominal pain in children with special emphasis on their clinical differentiation."}
{"id": "PMID:1079235", "title": "The influence of thyroxine and acclimation temperature on glycogen reserves of the frog Rana pipiens.", "content": "Leopard frogs were given daily injections of Na-l-thyroxine (2.0 mug/10 g body weight) for seven days, and control animals were given daily injections of the vehicle. During the period of treatment, half of the frogs were acclimated, in darkness and without food, at a constant temperature of 15 degrees C, whereas the remainder of the animals were acclimated at 25 degrees C. At the end of the seventh day, the frogs were killed by decapitation, and the glycogen concentration in samples of liver tissue and skeletal muscle was determined spectrophotometrically. Total hepatic glycogen of each frog subsequently was calculated from data on glycogen concentration and liver weight. Treatment of leopard frogs with thyroxine had no apparent effect on hepatic glycogen reserves of animals acclimated at 15 degrees C, but there was a striking reduction in glycogen content of livers from hyperthyroid frogs held at 25 degrees C. Mobilization of hepatic glycogen in thyroxine-treated animals held at 25 degrees C presumably was secondary to a general stimulation of oxidative metabolism. Treatment with thyroxine had no effect on the concentration of glycogen in skeletal muscle of leopard frogs held at either of the acclimation temperatures used in this study.", "contents": "The influence of thyroxine and acclimation temperature on glycogen reserves of the frog Rana pipiens. Leopard frogs were given daily injections of Na-l-thyroxine (2.0 mug/10 g body weight) for seven days, and control animals were given daily injections of the vehicle. During the period of treatment, half of the frogs were acclimated, in darkness and without food, at a constant temperature of 15 degrees C, whereas the remainder of the animals were acclimated at 25 degrees C. At the end of the seventh day, the frogs were killed by decapitation, and the glycogen concentration in samples of liver tissue and skeletal muscle was determined spectrophotometrically. Total hepatic glycogen of each frog subsequently was calculated from data on glycogen concentration and liver weight. Treatment of leopard frogs with thyroxine had no apparent effect on hepatic glycogen reserves of animals acclimated at 15 degrees C, but there was a striking reduction in glycogen content of livers from hyperthyroid frogs held at 25 degrees C. Mobilization of hepatic glycogen in thyroxine-treated animals held at 25 degrees C presumably was secondary to a general stimulation of oxidative metabolism. Treatment with thyroxine had no effect on the concentration of glycogen in skeletal muscle of leopard frogs held at either of the acclimation temperatures used in this study."}
{"id": "PMID:1079240", "title": "Single-unit responses to natural vestibular stimuli and eye movements in deep cerebellar nuclei of the alert rhesus monkey.", "content": "To study the possible role of the cerebellum in the vestibular-ocular reflex, extracellular responses of cerebellar nuclear neurons were recorded in awake monkeys during natural vestibular stimulation; 115 neurons in the fastigial nucleus responded to horizontal sinusoidal accelerations applied to the head by means of whole-body rotation. More than 75% of these cells were located in a distinct layer, 500 mum thick, in the rostral part of the fastigial nucleus; they were excited by contralateral horizontal angular acceleration and inhibited by ipsilateral rotation (type IIf neurons). The remaining 25% of the population were scattered more caudally in the nucleus, and were excited by ipsilateral rotation and inhibited by contralateral rotation (type If). All showed fairly high resting discharges, averaging 50 spikes/s. Sinusoidal horizontal rotation (0.2--4.8 HZ) produced clear periodic modulation of the firing rate of fastigial neurons, which was approximately sinusoidal about the resting rate at low frequencies. As the frequency of oscillation (and the applied acceleration) increased, the sinusoidal modulation of unit firing increased in amplitude; at high stimulus frequencies the firing rate was usually driven to zero during the inhibitory part of stimulus cycle, but did not saturate in the excitatory half leading to an increase in the mean firing rate. The maximum firing rates of fastigial neurons were related to the peak acceleration by a power function. At all stimulus frequencies, the peak firing frequency of fastigial neurons lagged the input angular acceleration. Maximum firing of most units occurred just prior to the maximum velocity of the head. The gain and phase lag of the averaged unit discharge relative to head acceleration were calculated by Fourier analysis, using the fundamental as a first approximation of the response. Over a 20-fold stimulus range (0.2--4.0 HZ), mean phage lags of type IIf unit responses with respect to the applied acceleration remained relatively constant; the phase lag at 0.9 HZ measured 62 plus or minus 13 degrees. This phase lag is very similar to that recorded from vestibular nerve fibers (15), suggesting that type IIf fastigial neurons provide an excitatory signal to the ipsilateral vestibular nuclei which is in phase with direct vestibular afferent input, although functionally opposite in sign. Over the same frequency range, the gain decreased at minus- 18 dB/decade. Our data suggests that the majority of fastigial neurons work in parallel with flocculus Purkinje cells to functionally inhibit type Iv neurons in the ipsilateral vestibular nuclei.", "contents": "Single-unit responses to natural vestibular stimuli and eye movements in deep cerebellar nuclei of the alert rhesus monkey. To study the possible role of the cerebellum in the vestibular-ocular reflex, extracellular responses of cerebellar nuclear neurons were recorded in awake monkeys during natural vestibular stimulation; 115 neurons in the fastigial nucleus responded to horizontal sinusoidal accelerations applied to the head by means of whole-body rotation. More than 75% of these cells were located in a distinct layer, 500 mum thick, in the rostral part of the fastigial nucleus; they were excited by contralateral horizontal angular acceleration and inhibited by ipsilateral rotation (type IIf neurons). The remaining 25% of the population were scattered more caudally in the nucleus, and were excited by ipsilateral rotation and inhibited by contralateral rotation (type If). All showed fairly high resting discharges, averaging 50 spikes/s. Sinusoidal horizontal rotation (0.2--4.8 HZ) produced clear periodic modulation of the firing rate of fastigial neurons, which was approximately sinusoidal about the resting rate at low frequencies. As the frequency of oscillation (and the applied acceleration) increased, the sinusoidal modulation of unit firing increased in amplitude; at high stimulus frequencies the firing rate was usually driven to zero during the inhibitory part of stimulus cycle, but did not saturate in the excitatory half leading to an increase in the mean firing rate. The maximum firing rates of fastigial neurons were related to the peak acceleration by a power function. At all stimulus frequencies, the peak firing frequency of fastigial neurons lagged the input angular acceleration. Maximum firing of most units occurred just prior to the maximum velocity of the head. The gain and phase lag of the averaged unit discharge relative to head acceleration were calculated by Fourier analysis, using the fundamental as a first approximation of the response. Over a 20-fold stimulus range (0.2--4.0 HZ), mean phage lags of type IIf unit responses with respect to the applied acceleration remained relatively constant; the phase lag at 0.9 HZ measured 62 plus or minus 13 degrees. This phase lag is very similar to that recorded from vestibular nerve fibers (15), suggesting that type IIf fastigial neurons provide an excitatory signal to the ipsilateral vestibular nuclei which is in phase with direct vestibular afferent input, although functionally opposite in sign. Over the same frequency range, the gain decreased at minus- 18 dB/decade. Our data suggests that the majority of fastigial neurons work in parallel with flocculus Purkinje cells to functionally inhibit type Iv neurons in the ipsilateral vestibular nuclei."}
{"id": "PMID:1079251", "title": "Hemophilus influenzae type B susceptibility to 17 antibiotics.", "content": "One hundred and one strains of Hemophilus influenzae type b isolated at the Children's Hospital Medical Center from blood or CSF and 18 strains known to be resistant to ampicillin of which 17 were supplied to us by others were tested for their sensitivity to 17 antibiotics. Two groups were defined according to their sensitivity to ampicillin. When 10-3 bacteria were applied, 18 strains isolated from patients with ampicillin treatment failures had a median minimum inhibitory concentrations of 3.1 mug/ml. At 10-6 bacteria the median MIC for the resistant strains increased 512-fold, whereas the sensitive strains increased two-fold. The most active antibiotics against sensitive and resistant strains were rifampin, chloramphenicol, gentamicin, rosamycin, and erythromycin with MICs ranging from 0.2-0.8 mug/ml at 10-3 bacteria and from 0.2-1.6 mug/ml at 10-6.", "contents": "Hemophilus influenzae type B susceptibility to 17 antibiotics. One hundred and one strains of Hemophilus influenzae type b isolated at the Children's Hospital Medical Center from blood or CSF and 18 strains known to be resistant to ampicillin of which 17 were supplied to us by others were tested for their sensitivity to 17 antibiotics. Two groups were defined according to their sensitivity to ampicillin. When 10-3 bacteria were applied, 18 strains isolated from patients with ampicillin treatment failures had a median minimum inhibitory concentrations of 3.1 mug/ml. At 10-6 bacteria the median MIC for the resistant strains increased 512-fold, whereas the sensitive strains increased two-fold. The most active antibiotics against sensitive and resistant strains were rifampin, chloramphenicol, gentamicin, rosamycin, and erythromycin with MICs ranging from 0.2-0.8 mug/ml at 10-3 bacteria and from 0.2-1.6 mug/ml at 10-6."}
{"id": "PMID:1079252", "title": "Childhood cirrhosis associated with alpha-1-antitrypsin deficiency. A genetic, biochemical, and morphologic study.", "content": "A study of 27 relatives of a child with fatal hepatic cirrhosis due to homozygous Z variant alpha-1-antitrypsin deficiency revealed 15 members with heterozygous MZ phenotypes. Levels of circulating alpha-1-antitrypsin and trypsin-inhibiting capacity were shown to be unreliable in identifying the heterozygous state, Pi typing being necessary for definitive diagnosis. The morphologic evolution of the hepatic changes in this condition have been studied, and the importance of the PAS stain in identification of the characteristic cytoplasmic bodies is stressed.", "contents": "Childhood cirrhosis associated with alpha-1-antitrypsin deficiency. A genetic, biochemical, and morphologic study. A study of 27 relatives of a child with fatal hepatic cirrhosis due to homozygous Z variant alpha-1-antitrypsin deficiency revealed 15 members with heterozygous MZ phenotypes. Levels of circulating alpha-1-antitrypsin and trypsin-inhibiting capacity were shown to be unreliable in identifying the heterozygous state, Pi typing being necessary for definitive diagnosis. The morphologic evolution of the hepatic changes in this condition have been studied, and the importance of the PAS stain in identification of the characteristic cytoplasmic bodies is stressed."}
{"id": "PMID:1079253", "title": "Characterization of nucleoside transport in hymenolepidid cestodes.", "content": "Three species of cestodes, Hymenolepis diminuta, H. citelli, and H. microstoma, were shown to transport nucleosides by a mediated process. In H. diminuta the Ki values of various competitive inhibitors led to the conclusion that at least 2 different loci are involved in the transport of nucleosides. One of these loci has greater specificity for the purine nucleosides, the other for pyrimidine nucleosides. No significant difference was observed in their affinity for ribo- or deoxyribonucleosides, although thymidine was slightly less effective as an inhibitor of ribonucleosides than was uridine. Transport by the pyrimidine nucleoside locus was stimulated by thymine, but not by hypoxanthine, whereas transport by the purine nucleoside locus was stimulated by hypoxanthine but not by thymine. Preloading the worms with thymine gave less stimulation of transport than did the presence of eoxgenous modulator. Efflux of previously accumulated nucleoside was not blocked by the presence of exogenous modulator. The presence of exgenous thymine enhanced the incorporation of uridine into the nonextractable pool. Thymine also stimulated uracil transport in H. citelli, but not in H. microstoma, and had no effect on uridine transport in either of these species. The results of transport and modulator studies on H. diminuta grown in hamsters were not different from the results with worms grown in rats indicating that the regulatory effects observed were an inherent function of the parasite and not the host.", "contents": "Characterization of nucleoside transport in hymenolepidid cestodes. Three species of cestodes, Hymenolepis diminuta, H. citelli, and H. microstoma, were shown to transport nucleosides by a mediated process. In H. diminuta the Ki values of various competitive inhibitors led to the conclusion that at least 2 different loci are involved in the transport of nucleosides. One of these loci has greater specificity for the purine nucleosides, the other for pyrimidine nucleosides. No significant difference was observed in their affinity for ribo- or deoxyribonucleosides, although thymidine was slightly less effective as an inhibitor of ribonucleosides than was uridine. Transport by the pyrimidine nucleoside locus was stimulated by thymine, but not by hypoxanthine, whereas transport by the purine nucleoside locus was stimulated by hypoxanthine but not by thymine. Preloading the worms with thymine gave less stimulation of transport than did the presence of eoxgenous modulator. Efflux of previously accumulated nucleoside was not blocked by the presence of exogenous modulator. The presence of exgenous thymine enhanced the incorporation of uridine into the nonextractable pool. Thymine also stimulated uracil transport in H. citelli, but not in H. microstoma, and had no effect on uridine transport in either of these species. The results of transport and modulator studies on H. diminuta grown in hamsters were not different from the results with worms grown in rats indicating that the regulatory effects observed were an inherent function of the parasite and not the host."}
{"id": "PMID:1079255", "title": "Nitrogenous cations as probes of permeation channels.", "content": "Nitrogenous cations may provide information-rich probes of cation-selective channels. Hence, for 52 nitrogenous cations we have used dilution potentials and biionic potentials to measure relative permeability coefficients (P's) across gallbladder epithelia of frog and rabbit, and have also determined the free-solution mobilities. Measured P's of most cations are uninfluenced by the presence of the netral form. The main permeation pathway for most hydrophilic cations is across the tight junctions. P's decrease with molecular size and increase with number of donor protons available for hydrogen-bond formation. Selectivity isotherms have been constructed from variation in P's due to pH or due to differences among individual animals. Both types of variation are consistent with the pattern expected from variation in electrostatic field strength of cation-binding sites. The isotherms permit P's to be re-expressed in a way that largely eliminates effects of species differences in field strength. Remaining species differences in P's are well fitted by a model of steric restriction, provided that one takes into account the effect of hydrogen bonding on molecular size. Rabbit gallbladder behaves as if it has narrower permeation channels than frog gallbladder. After correction for these steric effects, P is found to increase with number of donor protons nH up to four protons, with a steeper slope in rabbit than in frog gallbladder,  but is independent of nH from four to at least nine. Two groups of cations appear to permeate significantly via pathways other than tight junctions: oxycations, via polar pathways in epithelial cell membranes of rabbit but not frog gallbladder; and lipid-soluble cations, via membrane lipid. The results suggest that the cation-binding sites of gallbladder tight junction are acidic proton-acceptors that discriminate more sharply among proton donors than does water. Proton-rich solutes tend to be more permeant for two reasons: stronger binding energies to membrane proton-acceptor sites, and smaller effective size in a proton-acceptor environment. As deduced from comparisons of nitrogenous cation selectivity patterns, the permeation channel through gallbladder tight junction differs from nerve's sodium channel and artificial carriers and channels in its higher hydration and lower range of selectivity. Based on the steric analysis of nitrogenous cation permeation, one can correct alkali cation permeability coefficients for the effect of steric restriction.", "contents": "Nitrogenous cations as probes of permeation channels. Nitrogenous cations may provide information-rich probes of cation-selective channels. Hence, for 52 nitrogenous cations we have used dilution potentials and biionic potentials to measure relative permeability coefficients (P's) across gallbladder epithelia of frog and rabbit, and have also determined the free-solution mobilities. Measured P's of most cations are uninfluenced by the presence of the netral form. The main permeation pathway for most hydrophilic cations is across the tight junctions. P's decrease with molecular size and increase with number of donor protons available for hydrogen-bond formation. Selectivity isotherms have been constructed from variation in P's due to pH or due to differences among individual animals. Both types of variation are consistent with the pattern expected from variation in electrostatic field strength of cation-binding sites. The isotherms permit P's to be re-expressed in a way that largely eliminates effects of species differences in field strength. Remaining species differences in P's are well fitted by a model of steric restriction, provided that one takes into account the effect of hydrogen bonding on molecular size. Rabbit gallbladder behaves as if it has narrower permeation channels than frog gallbladder. After correction for these steric effects, P is found to increase with number of donor protons nH up to four protons, with a steeper slope in rabbit than in frog gallbladder,  but is independent of nH from four to at least nine. Two groups of cations appear to permeate significantly via pathways other than tight junctions: oxycations, via polar pathways in epithelial cell membranes of rabbit but not frog gallbladder; and lipid-soluble cations, via membrane lipid. The results suggest that the cation-binding sites of gallbladder tight junction are acidic proton-acceptors that discriminate more sharply among proton donors than does water. Proton-rich solutes tend to be more permeant for two reasons: stronger binding energies to membrane proton-acceptor sites, and smaller effective size in a proton-acceptor environment. As deduced from comparisons of nitrogenous cation selectivity patterns, the permeation channel through gallbladder tight junction differs from nerve's sodium channel and artificial carriers and channels in its higher hydration and lower range of selectivity. Based on the steric analysis of nitrogenous cation permeation, one can correct alkali cation permeability coefficients for the effect of steric restriction."}
{"id": "PMID:1079256", "title": "Quantitative relationship between active sodium transport, expansion of endoplasmic reticulum and specialized vacuoles (\"scalloped sacs\") in the outermost living cell layer of the frog skin epithelium (Rana temporaria)", "content": "When an isolated frog skin (Rana temporaria) is exposed to a hydrostatic pressure difference between inside and outside bathing solutions (inside pressure higher than outside) of 20-50 cm of H2O and if under these conditions the skin is short-circuited electrically, small \"vacuoles\" appear light-microscopically in the outermost living cell layer in the epithelium. The number of such \"vacuoles\" shows a linear dependency on the rate of active sodium transport as measured by the short-circuit current. Electron-microscopically, the \"vacuoles\" are interpreted as previously undescribed organelles, the \"scalloped sacs\" which are about 0.5 mu in diameter, with a wrinkled surface and bounded by a unit membrane. This organelle is in intimate contact with sacs and tubules of smooth endoplasmic reticulum. The observed increase in the number of scalloped sacs usually is accompanied by a significant expansion of the whole system of endoplasmic reticulum. Some of the \"vacuoles\" seen light-microscopically must indeed be expanded cisternae of endoplasmic reticulum. The findings are discussed in light of the possibility that the scalloped sacs and the endoplasmic reticulum may be involved in active transport of sodium ions.", "contents": "Quantitative relationship between active sodium transport, expansion of endoplasmic reticulum and specialized vacuoles (\"scalloped sacs\") in the outermost living cell layer of the frog skin epithelium (Rana temporaria). When an isolated frog skin (Rana temporaria) is exposed to a hydrostatic pressure difference between inside and outside bathing solutions (inside pressure higher than outside) of 20-50 cm of H2O and if under these conditions the skin is short-circuited electrically, small \"vacuoles\" appear light-microscopically in the outermost living cell layer in the epithelium. The number of such \"vacuoles\" shows a linear dependency on the rate of active sodium transport as measured by the short-circuit current. Electron-microscopically, the \"vacuoles\" are interpreted as previously undescribed organelles, the \"scalloped sacs\" which are about 0.5 mu in diameter, with a wrinkled surface and bounded by a unit membrane. This organelle is in intimate contact with sacs and tubules of smooth endoplasmic reticulum. The observed increase in the number of scalloped sacs usually is accompanied by a significant expansion of the whole system of endoplasmic reticulum. Some of the \"vacuoles\" seen light-microscopically must indeed be expanded cisternae of endoplasmic reticulum. The findings are discussed in light of the possibility that the scalloped sacs and the endoplasmic reticulum may be involved in active transport of sodium ions."}
{"id": "PMID:1079259", "title": "Acute renal failure in combat injuries.", "content": "The mortality in 25 patients suffering from post-combat injury acute renal failure (ARF) was 64%. Abdominal injuries were present in 17 patients with a mortality rate of 64.7%. Respiratory insufficiency occurred in 14 patients, jaundice occurred in 13, and septicemia in 10. The mean period of oliguria was high, 24.1 days per survivor, and the number of hemodialyses averaged 21.6 per survivor. It is concluded that the high mortality is primary due to the severity of the underlying injury itself and not due to the renal failure, that the ARF is more severe than in civilian injuries, as evidenced by a prolonged oliguric phase, and that frequent and intensive hemodialysis regimen is necessary for the elimination of deaths from uremia per se.", "contents": "Acute renal failure in combat injuries. The mortality in 25 patients suffering from post-combat injury acute renal failure (ARF) was 64%. Abdominal injuries were present in 17 patients with a mortality rate of 64.7%. Respiratory insufficiency occurred in 14 patients, jaundice occurred in 13, and septicemia in 10. The mean period of oliguria was high, 24.1 days per survivor, and the number of hemodialyses averaged 21.6 per survivor. It is concluded that the high mortality is primary due to the severity of the underlying injury itself and not due to the renal failure, that the ARF is more severe than in civilian injuries, as evidenced by a prolonged oliguric phase, and that frequent and intensive hemodialysis regimen is necessary for the elimination of deaths from uremia per se."}
{"id": "PMID:1079261", "title": "Lumbosacral nerve root avulsion: report of a case and review of the literature.", "content": "The 14th myelographically demonstrated case of lumbosacral nerve root avulsion is presented with a summary of the previously reported cases. In most cases lumbosacral nerve root avulsion is associated with pelvic fractures and sacroiliac dislocation, which cause a stretching force to be applied to the nerves of the lumbar and sacral plexuses, and in turn to the nerve roots intradurally. This force causes nerve root avulsion in the intradural course of the nerve root. The myelographic defect is a pseudomeningocele or diverticulum-like outpouching created by the tearing of the arachnoid covering of the nerve roots. Myelography clearly indicates nerve root avulsion and surgical exploration is not indicated.", "contents": "Lumbosacral nerve root avulsion: report of a case and review of the literature. The 14th myelographically demonstrated case of lumbosacral nerve root avulsion is presented with a summary of the previously reported cases. In most cases lumbosacral nerve root avulsion is associated with pelvic fractures and sacroiliac dislocation, which cause a stretching force to be applied to the nerves of the lumbar and sacral plexuses, and in turn to the nerve roots intradurally. This force causes nerve root avulsion in the intradural course of the nerve root. The myelographic defect is a pseudomeningocele or diverticulum-like outpouching created by the tearing of the arachnoid covering of the nerve roots. Myelography clearly indicates nerve root avulsion and surgical exploration is not indicated."}
{"id": "PMID:1079289", "title": "Cell-detaching activity mediated by an enzyme(s) obtained from human leukocyte granules.", "content": "Granule proteins have been isolated from human peripheral blood leukocytes and their effects on intact tissue culture cells have been determined. Three hours after the addition of the mixture of granule hydrolases, both HeLa cells and human newborn fibroblasts were detached from one another and from their growth surface, but they seemed to remain viable, since cell-bound -51Cr was retained. Further studies with HeLa cells indicated that after as long as 24 hours in the presence of these enzymes the cells remained viable as judged by several independent criteria. The enzyme activity responsible for cell detachment was subject to inhibition by various protease inhibitors. Its molecular size, as determined by gel filtration, was approximately 20,000 daltons. In addition, the naturally occurring serum inhibitor of this enzyme activity was characterized as either alpha(1)-antitrypsin or another protein with similar properties.", "contents": "Cell-detaching activity mediated by an enzyme(s) obtained from human leukocyte granules. Granule proteins have been isolated from human peripheral blood leukocytes and their effects on intact tissue culture cells have been determined. Three hours after the addition of the mixture of granule hydrolases, both HeLa cells and human newborn fibroblasts were detached from one another and from their growth surface, but they seemed to remain viable, since cell-bound -51Cr was retained. Further studies with HeLa cells indicated that after as long as 24 hours in the presence of these enzymes the cells remained viable as judged by several independent criteria. The enzyme activity responsible for cell detachment was subject to inhibition by various protease inhibitors. Its molecular size, as determined by gel filtration, was approximately 20,000 daltons. In addition, the naturally occurring serum inhibitor of this enzyme activity was characterized as either alpha(1)-antitrypsin or another protein with similar properties."}
{"id": "PMID:1079291", "title": "Risk of coronary surgery. Two hundred consecutive patients with no hospital deaths.", "content": "Revascularization surgery must carry a low morbidity and mortality rate to be a valid alternative treatment of obstructive coronary disease. A consecutive series of 200 patients underwent coronary bypasses at LIJ-HMC. The average age was 53.9 years (33 to 77) with 81.5 per cent of the patients men. Clinical presentation was as follows: 28 per cent were in New York Heart Association Class III and 72 per cent in Class IV (or unstable). Previous infarctions were documented in 37 per cent. Sixteen patients (8 per cent) had disease of the main left coronary artery. Single bypasses were placed in 37.5 per cent, double in 40.5 per cent, and triple or quadruple in 22 per cent. The rate of vein graft patency was 95.3 per cent (245 of 257 studied grafts). There were no hospital deaths in this consecutive group of 200 patients. There have been two late deaths after an average follow-up period of 16 months.", "contents": "Risk of coronary surgery. Two hundred consecutive patients with no hospital deaths. Revascularization surgery must carry a low morbidity and mortality rate to be a valid alternative treatment of obstructive coronary disease. A consecutive series of 200 patients underwent coronary bypasses at LIJ-HMC. The average age was 53.9 years (33 to 77) with 81.5 per cent of the patients men. Clinical presentation was as follows: 28 per cent were in New York Heart Association Class III and 72 per cent in Class IV (or unstable). Previous infarctions were documented in 37 per cent. Sixteen patients (8 per cent) had disease of the main left coronary artery. Single bypasses were placed in 37.5 per cent, double in 40.5 per cent, and triple or quadruple in 22 per cent. The rate of vein graft patency was 95.3 per cent (245 of 257 studied grafts). There were no hospital deaths in this consecutive group of 200 patients. There have been two late deaths after an average follow-up period of 16 months."}
{"id": "PMID:1079292", "title": "Postperfusion stenosis of the common left coronary artery.", "content": "Late stenosis is a rare but potentially lethal complication of coronary perfusion. In 3 patients, anginal pain appeared 2 to 6 months after aortic valve surgery. Coronary angiography was performed because of rapid evolution of symptoms. Severe stenosis of the common left coronary artery was shown in all 3 cases. Immediate aorto-coronary bypass was undertaken with good results. Graft patency was demonstrated in 2 cases, 6 and 7 months after the operations.", "contents": "Postperfusion stenosis of the common left coronary artery. Late stenosis is a rare but potentially lethal complication of coronary perfusion. In 3 patients, anginal pain appeared 2 to 6 months after aortic valve surgery. Coronary angiography was performed because of rapid evolution of symptoms. Severe stenosis of the common left coronary artery was shown in all 3 cases. Immediate aorto-coronary bypass was undertaken with good results. Graft patency was demonstrated in 2 cases, 6 and 7 months after the operations."}
{"id": "PMID:1079293", "title": "A comparison of membrane and bubble oxygenation as used in cardiopulmonary bypass in patients. The importance of pericardial blood as a source of hemolysis.", "content": "A prospective clinical study involving more than 500 patients was designed to compare the Land\u00e9-Edwards membrane oxygenator and the Bentley bubble oxygenator. First, the importance of exposure of blood to the pericardium as the major source of hemolysis during open-heart surgery was confirmed. Because of this finding, we included in this study only those patients in whom the blood spilled into the pericardium was not returned to the pump. Under these circumstances, we found that hemolysis was relatively low in patients oxygenated with the membrane oxygenator.", "contents": "A comparison of membrane and bubble oxygenation as used in cardiopulmonary bypass in patients. The importance of pericardial blood as a source of hemolysis. A prospective clinical study involving more than 500 patients was designed to compare the Land\u00e9-Edwards membrane oxygenator and the Bentley bubble oxygenator. First, the importance of exposure of blood to the pericardium as the major source of hemolysis during open-heart surgery was confirmed. Because of this finding, we included in this study only those patients in whom the blood spilled into the pericardium was not returned to the pump. Under these circumstances, we found that hemolysis was relatively low in patients oxygenated with the membrane oxygenator."}
{"id": "PMID:1079299", "title": "Maternal deaths in Australia compared with England and Wales from 1967 to 1969.", "content": "A comparison of the main causes of maternal death in Australia and in England and Wales over the same three-year period from 1967 to 1969 was undertaken, based on the trienneal reports from the two countires. In this triennium the maternal mortality rates (maternal deaths due to pregnancy and childbirth and not including associated maternal deaths) of the two countries were very similar, that of Australia being 0.23 per 1,000 and that of England and Wales being 0.21 per 1,000. These figures include death due to abortion, and both show an improvement from the previous triennium. Comparison of the figures still reveals higher death rates from haemorrhage, pulmonary embolism and preeclampsia in Australia than in England and Wales, but this is largely balanced by the higher death rate from abortion in England and Wales. In 1972 Neil and Townsend compared the first report on maternal deaths in the Commonwealth of Australia with the fifth report on maternal deaths in England and Wales, covering the triennium 1964 to 1966. The purpose of this article is to compare the reports on the triennium 1967 to 1969 (Beischer et alii, 1972); Report on Confidential Enquiries into Maternal Deaths in England and Wales, 1967-1969) and to comment on the improvements which have occurred in this triennium.", "contents": "Maternal deaths in Australia compared with England and Wales from 1967 to 1969. A comparison of the main causes of maternal death in Australia and in England and Wales over the same three-year period from 1967 to 1969 was undertaken, based on the trienneal reports from the two countires. In this triennium the maternal mortality rates (maternal deaths due to pregnancy and childbirth and not including associated maternal deaths) of the two countries were very similar, that of Australia being 0.23 per 1,000 and that of England and Wales being 0.21 per 1,000. These figures include death due to abortion, and both show an improvement from the previous triennium. Comparison of the figures still reveals higher death rates from haemorrhage, pulmonary embolism and preeclampsia in Australia than in England and Wales, but this is largely balanced by the higher death rate from abortion in England and Wales. In 1972 Neil and Townsend compared the first report on maternal deaths in the Commonwealth of Australia with the fifth report on maternal deaths in England and Wales, covering the triennium 1964 to 1966. The purpose of this article is to compare the reports on the triennium 1967 to 1969 (Beischer et alii, 1972); Report on Confidential Enquiries into Maternal Deaths in England and Wales, 1967-1969) and to comment on the improvements which have occurred in this triennium."}
{"id": "PMID:1079322", "title": "Serum immunoglobulins in the nephrotic syndrome. A possible cause of minimal-change nephrotic syndrome.", "content": "To assess immunologic factors in the pathogenesis of idiopathic minimal-change nephrotic syndrome (INS), serum immunoglobulin concentrations were measured in 37 children with this syndrome and compared with those found in 36 with nephrotic syndrome secondary to chronic glomerulonephritis (CGN). Serum IgG and IgA levels were significantly reduced in nephrotic patients with either INS or CGN, IgG averaging 18.5 and 25.9 per cent of normal (P less than 0.001) and IgA 59.8 and 44.1 per cent of normal (P less than 0.01) respectively. Values increased after treatment of INS with prednisone, but mean values remained low. Serum IgM concentrations in INS averaged more than twice normal before, during, and after successful treatment with steroids. Patients with CGN did not have equivalent elevations of serum IgM. Thus, the primary defect in INS may be immunologic and could consist of deficiency in the T-cell function that mediates conversion of IgM synthesis to IgG synthesis.", "contents": "Serum immunoglobulins in the nephrotic syndrome. A possible cause of minimal-change nephrotic syndrome. To assess immunologic factors in the pathogenesis of idiopathic minimal-change nephrotic syndrome (INS), serum immunoglobulin concentrations were measured in 37 children with this syndrome and compared with those found in 36 with nephrotic syndrome secondary to chronic glomerulonephritis (CGN). Serum IgG and IgA levels were significantly reduced in nephrotic patients with either INS or CGN, IgG averaging 18.5 and 25.9 per cent of normal (P less than 0.001) and IgA 59.8 and 44.1 per cent of normal (P less than 0.01) respectively. Values increased after treatment of INS with prednisone, but mean values remained low. Serum IgM concentrations in INS averaged more than twice normal before, during, and after successful treatment with steroids. Patients with CGN did not have equivalent elevations of serum IgM. Thus, the primary defect in INS may be immunologic and could consist of deficiency in the T-cell function that mediates conversion of IgM synthesis to IgG synthesis."}
{"id": "PMID:1079332", "title": "T-lymphocytes and serum inhibitors of cell-mediated immunity in renal insufficiency.", "content": "In acute as well as chronic renal insufficiency significant immunological abnormalities were found: diminution of delayed hypersensitivity skin reactions, lymphopenia, reduction of the absolute but not relative number of peripheral blood T-lymphocytes. Responses of lymphocytes from uremic patients to PHA, allogeneic cells, or antigens were normal when lymphocyte cultures were preformed in allologous serum from a healthy individual. Sera from uremic patients had an inhibitory or toxic effect on stimulation of normal lymphocytes. Such an in vitro inhibitory activity was found not only in the whole serum but also when certain substances retained in renal failure (methylguanidine, larger molecules, etc.) were added in the lymphocyte cultures.", "contents": "T-lymphocytes and serum inhibitors of cell-mediated immunity in renal insufficiency. In acute as well as chronic renal insufficiency significant immunological abnormalities were found: diminution of delayed hypersensitivity skin reactions, lymphopenia, reduction of the absolute but not relative number of peripheral blood T-lymphocytes. Responses of lymphocytes from uremic patients to PHA, allogeneic cells, or antigens were normal when lymphocyte cultures were preformed in allologous serum from a healthy individual. Sera from uremic patients had an inhibitory or toxic effect on stimulation of normal lymphocytes. Such an in vitro inhibitory activity was found not only in the whole serum but also when certain substances retained in renal failure (methylguanidine, larger molecules, etc.) were added in the lymphocyte cultures."}
{"id": "PMID:1079333", "title": "[Data obtained in more than 20 years of extensive use of Salvioli's diffusing anti-tuberculosis killed vaccine in Bologna].", "content": "V.D.S. (Killed T.B. vaccine, Salvioli disperdent) has been administered intradermally to the newborn in Bologna for over 20 yr. It causes a distinct reaction, lasting several weeks, in the injection site and its associated tissues. The alllergy thus constituted is evidence of a highly effective, timely and lasting defence reaction, accompanied by a marked increase in paraspecific reactivity directed to various other sources of infection. These features have been observed and recorded over the long period in which V.D.S. has been widely employed in voluntary vaccination of the newborn in Bologna.", "contents": "[Data obtained in more than 20 years of extensive use of Salvioli's diffusing anti-tuberculosis killed vaccine in Bologna]. V.D.S. (Killed T.B. vaccine, Salvioli disperdent) has been administered intradermally to the newborn in Bologna for over 20 yr. It causes a distinct reaction, lasting several weeks, in the injection site and its associated tissues. The alllergy thus constituted is evidence of a highly effective, timely and lasting defence reaction, accompanied by a marked increase in paraspecific reactivity directed to various other sources of infection. These features have been observed and recorded over the long period in which V.D.S. has been widely employed in voluntary vaccination of the newborn in Bologna."}
{"id": "PMID:1079335", "title": "Vagal responses to vestibular stimulation in the cat.", "content": "The effects of vestibular stimuli on the vagal efferent complex were examined in cats under chloralose anasthesia. The results showed that two functionally distinct groups of vagal efferent fibres are excited by vestibular stimuli. The neural complex involved in the delayed vestibulo-vagal response (latency of 15-22 msec) lies at the orbital surface of the cerebral cortex. The early response (latency of 3-5 msec) originates in the medulla oblongata. The reaction of some vagal units to vestibular polarization is quite similar to that of vestibular units and suggests that primary vestibular fibres reach the ipsilateral vagal efferent neurons. The present experiments give further evidence that the vestibular complex exerts a controlling influence on the cardiovascular system.", "contents": "Vagal responses to vestibular stimulation in the cat. The effects of vestibular stimuli on the vagal efferent complex were examined in cats under chloralose anasthesia. The results showed that two functionally distinct groups of vagal efferent fibres are excited by vestibular stimuli. The neural complex involved in the delayed vestibulo-vagal response (latency of 15-22 msec) lies at the orbital surface of the cerebral cortex. The early response (latency of 3-5 msec) originates in the medulla oblongata. The reaction of some vagal units to vestibular polarization is quite similar to that of vestibular units and suggests that primary vestibular fibres reach the ipsilateral vagal efferent neurons. The present experiments give further evidence that the vestibular complex exerts a controlling influence on the cardiovascular system."}
{"id": "PMID:1079336", "title": "Hemophilus meningitis: comparison of H. influenzae and H. parainfluenzae.", "content": "Eleven cases of Hemophilus meningitis seen in the last year are reported. Three were H. parainfluenzae and eight were H. influenzae. The number of patients is small but probably indicates that there is little difference in the clinical or laboratory data in meningitis caused by these two organisms. The children with H. parainfluenzae tended to be less anemic and had more normal admission spinal fluid sugars and proteins, but this was probably because these patients were older and had shorter prodromes. As more cases of H. parainfluenzae meningitis are noted, it seems likely that there will not be any difference in the clinical or laboratory data. Two of the three cases of H. parainfluenzae meningitis had the complication of ataxia secondary to severe bilateral vestibular deficits. Finally, it should be noted that these two organisms will not be distinguished by routine bacteriological studies, and that differentiation must be carried out by an experimental laboratory.", "contents": "Hemophilus meningitis: comparison of H. influenzae and H. parainfluenzae. Eleven cases of Hemophilus meningitis seen in the last year are reported. Three were H. parainfluenzae and eight were H. influenzae. The number of patients is small but probably indicates that there is little difference in the clinical or laboratory data in meningitis caused by these two organisms. The children with H. parainfluenzae tended to be less anemic and had more normal admission spinal fluid sugars and proteins, but this was probably because these patients were older and had shorter prodromes. As more cases of H. parainfluenzae meningitis are noted, it seems likely that there will not be any difference in the clinical or laboratory data. Two of the three cases of H. parainfluenzae meningitis had the complication of ataxia secondary to severe bilateral vestibular deficits. Finally, it should be noted that these two organisms will not be distinguished by routine bacteriological studies, and that differentiation must be carried out by an experimental laboratory."}
{"id": "PMID:1079339", "title": "[Clinical study and course of 102 cases of chronic pancreatitis].", "content": "Symptomatology, signs and clinical course were studied in 102 cases of chronic pancreatics (CP), confirmed on the basis of strict criteria. Calcifications were present in 72 cases and chronic alcoholism in 81 cases. In seven patients acute pancreatitis, confirmed at surgery, appeared to have preceeded the development of CP. The only clinical difference between calcifying and non-calcifying forms was a higher incidence of steatorrhea in the first group. Cholelithiasis was present in 17 p.cent, alcoholic cirrhosis in 17 p.cent and a peptic ulcer in 24 p.cent of the whole series. Seventy two patients underwent surgery either for a complication in their course (attack of acute pancreatitis, pancreatic pseudo-cyst, cholestatic jaundice, segmental portal hypertension or antro-pyloroduodenal stenosis) or for cholelithiasis. Course assessed in 70 patients studied for more than 2 years was regressive in almost 60 p.cent of cases. There was no difference in the frequency of regression in those patients undergoing surgery and those treated medically only. In all cases, abstention from alcohol is an essential factor to a favourable result.", "contents": "[Clinical study and course of 102 cases of chronic pancreatitis]. Symptomatology, signs and clinical course were studied in 102 cases of chronic pancreatics (CP), confirmed on the basis of strict criteria. Calcifications were present in 72 cases and chronic alcoholism in 81 cases. In seven patients acute pancreatitis, confirmed at surgery, appeared to have preceeded the development of CP. The only clinical difference between calcifying and non-calcifying forms was a higher incidence of steatorrhea in the first group. Cholelithiasis was present in 17 p.cent, alcoholic cirrhosis in 17 p.cent and a peptic ulcer in 24 p.cent of the whole series. Seventy two patients underwent surgery either for a complication in their course (attack of acute pancreatitis, pancreatic pseudo-cyst, cholestatic jaundice, segmental portal hypertension or antro-pyloroduodenal stenosis) or for cholelithiasis. Course assessed in 70 patients studied for more than 2 years was regressive in almost 60 p.cent of cases. There was no difference in the frequency of regression in those patients undergoing surgery and those treated medically only. In all cases, abstention from alcohol is an essential factor to a favourable result."}
{"id": "PMID:1079346", "title": "Thymus-derived rat lymphocyte receptor for cell surface antigens is a nonserologically defined product of the major histocompatibility gene complex.", "content": "Recognition of cell surface antigens by nonsensitized thymus-derived rat lymphocytes is not affected by antisera against immunoglobulin, yet can be effectively blocked by treatment of the lymphocytes with alloantisera raised against lymphoid cells. However, allo antisera induced by nonlymphoid cells do not inhibit recognition. Adsorption of blocking antisera on immunoglobulin-Sepharose columns does not decrease their blocking activity. Absorption of blocking alloantisera with liver and kidney homogenates removes the cytotoxic alloantibodies, yet does not affect the capacity of the antisera to block recognition. Anti-H1-1 alloantisera block antigen recognition only of lymphocytes of strains that share the H1-1 locus. These results suggest that the constant part of the thymus-derived lymphocyte receptor for cell-surface antigens is a product of the rat major histocompatibility locus, which is not identical with the serologically defined antigens.", "contents": "Thymus-derived rat lymphocyte receptor for cell surface antigens is a nonserologically defined product of the major histocompatibility gene complex. Recognition of cell surface antigens by nonsensitized thymus-derived rat lymphocytes is not affected by antisera against immunoglobulin, yet can be effectively blocked by treatment of the lymphocytes with alloantisera raised against lymphoid cells. However, allo antisera induced by nonlymphoid cells do not inhibit recognition. Adsorption of blocking antisera on immunoglobulin-Sepharose columns does not decrease their blocking activity. Absorption of blocking alloantisera with liver and kidney homogenates removes the cytotoxic alloantibodies, yet does not affect the capacity of the antisera to block recognition. Anti-H1-1 alloantisera block antigen recognition only of lymphocytes of strains that share the H1-1 locus. These results suggest that the constant part of the thymus-derived lymphocyte receptor for cell-surface antigens is a product of the rat major histocompatibility locus, which is not identical with the serologically defined antigens."}
{"id": "PMID:1079347", "title": "Differentiation of population of peripheral blood lymphocytes into cells bearing sheep erythrocyte receptors in vitro by human thymic extract.", "content": "A small population of human marrow cells has been shown to be differentiated in vitro by thymic extract into cells bearing T-lymphocyte (thymus-derived lymphocyte) characteristics. By a similar method, the differentiation of human peripheral blood lymphocytes has been studied. A discontinuous gradient of bovine serum albumin was used to isolate lymphocytes into four layers and cells from layers I and III demonstrated the greatest potential for differentiation by human thymic extract. Appearance of T-lymphocyte characteristics was recognized by the spontaneous E-rosette technique with sheep erythrocytes. Ability of human marrow cells to be differentiated under the influence of human thymic extract was abolished by specific inhibitors of nucleic acid synthesis, however, had no inhibitory effect on the maturation of peripheral blood lymphocytes during a 2 hr incubation with human thymic extract but puromycin, an inhibitor of protein synthesis, abolished this differentiative step in cells of layer I. It is suggested from these studies that many of the cells in peripheral blood that are differentiable by thymic extract are at a stage of maturation more advanced than those in human marrow that are also differentiable by thymic extract.", "contents": "Differentiation of population of peripheral blood lymphocytes into cells bearing sheep erythrocyte receptors in vitro by human thymic extract. A small population of human marrow cells has been shown to be differentiated in vitro by thymic extract into cells bearing T-lymphocyte (thymus-derived lymphocyte) characteristics. By a similar method, the differentiation of human peripheral blood lymphocytes has been studied. A discontinuous gradient of bovine serum albumin was used to isolate lymphocytes into four layers and cells from layers I and III demonstrated the greatest potential for differentiation by human thymic extract. Appearance of T-lymphocyte characteristics was recognized by the spontaneous E-rosette technique with sheep erythrocytes. Ability of human marrow cells to be differentiated under the influence of human thymic extract was abolished by specific inhibitors of nucleic acid synthesis, however, had no inhibitory effect on the maturation of peripheral blood lymphocytes during a 2 hr incubation with human thymic extract but puromycin, an inhibitor of protein synthesis, abolished this differentiative step in cells of layer I. It is suggested from these studies that many of the cells in peripheral blood that are differentiable by thymic extract are at a stage of maturation more advanced than those in human marrow that are also differentiable by thymic extract."}
{"id": "PMID:1079348", "title": "Repair of ultraviolet irradiation damage to a cytoplasmic component required for neural induction in the amphibian egg.", "content": "Localized ultraviolet irradiation of the amphibian egg destroys a cytoplasmic component that is required for neural induction. Destruction of that component severely diminishes the inducing capacity of the dorsal lip at gastrulation, as determined by embryological assays. Repair of the ultraviolet lesion can be achieved by replacing the dorsal lip of the irradiated embyro with a lip from an unirradiated embryo.", "contents": "Repair of ultraviolet irradiation damage to a cytoplasmic component required for neural induction in the amphibian egg. Localized ultraviolet irradiation of the amphibian egg destroys a cytoplasmic component that is required for neural induction. Destruction of that component severely diminishes the inducing capacity of the dorsal lip at gastrulation, as determined by embryological assays. Repair of the ultraviolet lesion can be achieved by replacing the dorsal lip of the irradiated embyro with a lip from an unirradiated embryo."}
{"id": "PMID:1079349", "title": "Presynaptic action of barbiturates in the frog spinal cord.", "content": "The action of pentobarbital on primary afferents of the isolated frog spinal cord was analyzed with sucrose gap and intracellular recordings techniques. Pentobarbital in concentrations generally considered to be in the anesthetic range greatly prolonged presynaptic inhibition and also depolarized primary afferents. The depolarization was accompanied by an increase in excitability and resulted from activation of gamma-aminobutyric acid receptors, possibly by a direct action on these receptors, since the depolarization was reversibly blocked by gamma-aminobutyric acid, but not by glycine, antagonists, and magnesium ions. Furthermore, dorsal root ganglion cells exhibited a reduced sensitivity to both gamma-aminobutyric acid and pentobarbital after a \"desensitizing\" dose of gamma aminobutyric acid. The prolongation of presynaptic inhibition and the activation of gamma-aminobutyric acid receptors on primary afferents by pentobarbital should act to reduce the amount of transmitter released from the first synapse in sensory pathways.", "contents": "Presynaptic action of barbiturates in the frog spinal cord. The action of pentobarbital on primary afferents of the isolated frog spinal cord was analyzed with sucrose gap and intracellular recordings techniques. Pentobarbital in concentrations generally considered to be in the anesthetic range greatly prolonged presynaptic inhibition and also depolarized primary afferents. The depolarization was accompanied by an increase in excitability and resulted from activation of gamma-aminobutyric acid receptors, possibly by a direct action on these receptors, since the depolarization was reversibly blocked by gamma-aminobutyric acid, but not by glycine, antagonists, and magnesium ions. Furthermore, dorsal root ganglion cells exhibited a reduced sensitivity to both gamma-aminobutyric acid and pentobarbital after a \"desensitizing\" dose of gamma aminobutyric acid. The prolongation of presynaptic inhibition and the activation of gamma-aminobutyric acid receptors on primary afferents by pentobarbital should act to reduce the amount of transmitter released from the first synapse in sensory pathways."}
{"id": "PMID:1079351", "title": "Cross-reactivity between human thymus and mouse lymphoid tissues, as revealed by rabbit antiserum against human brain.", "content": "Rabbit anti-human brain antisera were found cytotoxic for mouse thymus, lymph node, and spleen cells. Absorption with human thymus cells removed some of the cytotoxic antibodies. Cross-reactions were also detected among human brain and thymus and mouse brain, liver, and erythrocytes.", "contents": "Cross-reactivity between human thymus and mouse lymphoid tissues, as revealed by rabbit antiserum against human brain. Rabbit anti-human brain antisera were found cytotoxic for mouse thymus, lymph node, and spleen cells. Absorption with human thymus cells removed some of the cytotoxic antibodies. Cross-reactions were also detected among human brain and thymus and mouse brain, liver, and erythrocytes."}
{"id": "PMID:1079352", "title": "Transcatheter arterial embolization in the management of bleeding in the cancer patient.", "content": "Selective transcatheter arterial embolization was performed in 7 cancer patients with gastrointestinal or uterine bleeding. Most were bleeding from neoplastic tissue and were refractory to conservative management. Complete and permanent cessation of bleeding was obtained in 5 patients, including 3 with gastric bleeding, 1 with colonic bleeding, and in 1 with uterine bleeding. Successful arterial embolization allowed further treatment of the underlying disease which was particularly critical as these patients were poor surgical candidates. No significant or prolonged side effects were noted.", "contents": "Transcatheter arterial embolization in the management of bleeding in the cancer patient. Selective transcatheter arterial embolization was performed in 7 cancer patients with gastrointestinal or uterine bleeding. Most were bleeding from neoplastic tissue and were refractory to conservative management. Complete and permanent cessation of bleeding was obtained in 5 patients, including 3 with gastric bleeding, 1 with colonic bleeding, and in 1 with uterine bleeding. Successful arterial embolization allowed further treatment of the underlying disease which was particularly critical as these patients were poor surgical candidates. No significant or prolonged side effects were noted."}
{"id": "PMID:1079368", "title": "[Value of HL-A groups in diagnosis in rheumatology].", "content": "Since 1972, several relations have been demonstrated between some HL-A antigens and some articular diseases. The W27 antigen frequency is highly increased in ankylosing spondylitis (88%) and in Reiter disease (78%) compared with controls (5%). In peripheral forms of psoriatic rheumatism, the W17 and HL-A13 antigens are more frequent (24% and 15%) than in healthy subjects (4% and 5%). In central forms of psoriatic rheumatism, there is a relation with W27 (48%) and still, we do not know if this association concerns only spondylitis or also sacro-iletis. The HL-A typing may be useful for the diagnosis of some rheumatologic diseases, when for the diagnosis of ankylosing spondylitis. The relation between W27 and ankylosing spondylitis is cleanly stronger than that between Waaler-Rose reaction and rheumatoid arthritis.", "contents": "[Value of HL-A groups in diagnosis in rheumatology]. Since 1972, several relations have been demonstrated between some HL-A antigens and some articular diseases. The W27 antigen frequency is highly increased in ankylosing spondylitis (88%) and in Reiter disease (78%) compared with controls (5%). In peripheral forms of psoriatic rheumatism, the W17 and HL-A13 antigens are more frequent (24% and 15%) than in healthy subjects (4% and 5%). In central forms of psoriatic rheumatism, there is a relation with W27 (48%) and still, we do not know if this association concerns only spondylitis or also sacro-iletis. The HL-A typing may be useful for the diagnosis of some rheumatologic diseases, when for the diagnosis of ankylosing spondylitis. The relation between W27 and ankylosing spondylitis is cleanly stronger than that between Waaler-Rose reaction and rheumatoid arthritis."}
{"id": "PMID:1079369", "title": "[W27 HL-A antigen. Diagnostic value in rheumatology].", "content": "The authors studied the tissue groups in a first series of patients with properly diagnosed rheumatic disorders. They found HL-A W27 antigen in 84 percent of 50 cases of primitive ankylosing spondylarthritis in adults, in 33 percent of the 50 cases of Fiessinger-Leroy-Reiter syndrome, in 20 percent of the 40 cases of psoriatic polyarthritis (50 percent of the 12 cases with radiological sacroiliac involvement) and in 66 percent of the 6 cases of spondylarthritis associated with haemorrhagic rectocolitis. In a second series of 45 patients with unclassifiable inflammatory rheumatism, only the W27 antigen tested for and it was found in 33 percent of the patients. On the basis of these observations, which were compared with those in other publications, the authors confirm the absence of a clear correlation between the pathological picture and the presence of the W27 antigen. They discuss the significance of the association between the W27 antigen and the disease, and underline the diagnostic and sometimes the prognostic value of the W27 test in rheumatology.", "contents": "[W27 HL-A antigen. Diagnostic value in rheumatology]. The authors studied the tissue groups in a first series of patients with properly diagnosed rheumatic disorders. They found HL-A W27 antigen in 84 percent of 50 cases of primitive ankylosing spondylarthritis in adults, in 33 percent of the 50 cases of Fiessinger-Leroy-Reiter syndrome, in 20 percent of the 40 cases of psoriatic polyarthritis (50 percent of the 12 cases with radiological sacroiliac involvement) and in 66 percent of the 6 cases of spondylarthritis associated with haemorrhagic rectocolitis. In a second series of 45 patients with unclassifiable inflammatory rheumatism, only the W27 antigen tested for and it was found in 33 percent of the patients. On the basis of these observations, which were compared with those in other publications, the authors confirm the absence of a clear correlation between the pathological picture and the presence of the W27 antigen. They discuss the significance of the association between the W27 antigen and the disease, and underline the diagnostic and sometimes the prognostic value of the W27 test in rheumatology."}
{"id": "PMID:1079371", "title": "Rapid changes in shape and cell architecture of isolated fragments of amphibian embryonic tissues as an experimental model of morphogenesis.", "content": "Changes in the shape and cell architecture of pieces of epithelial and neural ectoderm, mesoderm, neural tube, and combined ectomesodermal fragments from embryos of Rana temporaria 0-60 min after isolation were studied. The fragments were capable of changing their shape quickly (actually during separation) or after a latent period of several minutes. Rapid deformations were not prevented by cooling or by moderate doses of cyanide; as a rule they were connected with contraction of the surface area of the cells of the fragment and they can be regarded as relaxation to forms with lower mechanical energy. The direction of the deformation usually coincides with the subsequent normal morphogenesis of the particular anlage. Deformations with a latent period are suppressed by cooling and by the addition of cyanide, which lead to an increase in the surface area of individual cells, but they reduce the total surface area of the fragment. The shape of the fragments becomes more complex: they become irregularly twisted, they form folds, and they separate into spherical regions with stretched surfaces (\"drops\"). These processes are connected with the performance of positive mechanical work by the intracellular contractile systems. The reasons why the fragments become more complex in shape are discussed.", "contents": "Rapid changes in shape and cell architecture of isolated fragments of amphibian embryonic tissues as an experimental model of morphogenesis. Changes in the shape and cell architecture of pieces of epithelial and neural ectoderm, mesoderm, neural tube, and combined ectomesodermal fragments from embryos of Rana temporaria 0-60 min after isolation were studied. The fragments were capable of changing their shape quickly (actually during separation) or after a latent period of several minutes. Rapid deformations were not prevented by cooling or by moderate doses of cyanide; as a rule they were connected with contraction of the surface area of the cells of the fragment and they can be regarded as relaxation to forms with lower mechanical energy. The direction of the deformation usually coincides with the subsequent normal morphogenesis of the particular anlage. Deformations with a latent period are suppressed by cooling and by the addition of cyanide, which lead to an increase in the surface area of individual cells, but they reduce the total surface area of the fragment. The shape of the fragments becomes more complex: they become irregularly twisted, they form folds, and they separate into spherical regions with stretched surfaces (\"drops\"). These processes are connected with the performance of positive mechanical work by the intracellular contractile systems. The reasons why the fragments become more complex in shape are discussed."}
{"id": "PMID:1079372", "title": "Behavior of sperm nuclei injected into intact ripening and ripe toad oocytes and into oocytes ripening after removal of the germinal vesicle.", "content": "The behavior of frog sperm nuclei (obtained by treating a sperm suspension with pronase) in the cytoplasm of intact ripening and ripe toad oocytes and in the cytoplasm of oocytes ripening after removal of the germinal vesicle was studied. Sperm nuclei treated with pronase in the cytoplasm of intact ripe oocytes behave like the nuclei of sperm cells penetrating during normal fertilization: they increase in volume, synthesize DNA, and start to divide. They undergo no changes in the cytoplasm of oocytes ripening in the absence of karyoplasm. Transformations of sperm nuclei into pronuclei and DNA synthesis in them are found initially at the metaphase stage of the second maturation division.", "contents": "Behavior of sperm nuclei injected into intact ripening and ripe toad oocytes and into oocytes ripening after removal of the germinal vesicle. The behavior of frog sperm nuclei (obtained by treating a sperm suspension with pronase) in the cytoplasm of intact ripening and ripe toad oocytes and in the cytoplasm of oocytes ripening after removal of the germinal vesicle was studied. Sperm nuclei treated with pronase in the cytoplasm of intact ripe oocytes behave like the nuclei of sperm cells penetrating during normal fertilization: they increase in volume, synthesize DNA, and start to divide. They undergo no changes in the cytoplasm of oocytes ripening in the absence of karyoplasm. Transformations of sperm nuclei into pronuclei and DNA synthesis in them are found initially at the metaphase stage of the second maturation division."}
{"id": "PMID:1079374", "title": "Prophylactic anticoagulation in total hip replacement.", "content": "In the prevention of thromboembolic complications following elective total hip operations, warfarin is a safe and effective agent which causes minimal bleeding complications, if the drug is first administered on the first postoperative day and thereafter continued in various dosages to maintain a prothrombin time between one and one and one-half and two times that of the control. Dextran also is effective in preventing thromboembolic complications but causes more bleeding problems, especially when administered intraoperatively.", "contents": "Prophylactic anticoagulation in total hip replacement. In the prevention of thromboembolic complications following elective total hip operations, warfarin is a safe and effective agent which causes minimal bleeding complications, if the drug is first administered on the first postoperative day and thereafter continued in various dosages to maintain a prothrombin time between one and one and one-half and two times that of the control. Dextran also is effective in preventing thromboembolic complications but causes more bleeding problems, especially when administered intraoperatively."}
{"id": "PMID:1079375", "title": "Transmyocardial left-to-right shunt complicating acute inferior wall myocardial infarction after aortocoronary bypass.", "content": "A 61-year-old woman suffered an inferior wall myocardial infarction on the third day following aortocoronary bypass surgery. Her condition subsequently deteriorated and she was not responsive to vigorous conventional therapy. On the twenty-first postoperative day, selective coronary arteriography revealed a patent circumflex bypass graft and an occluded right coronary artery bypass graft. Left ventricular injection showed slight dilation of the ventricle and a reduced ejection fraction. A dissection from the left ventricular cavity into the myocardium, across the septum near the apex of the left ventricle with rupture into the right ventricle, was seen functioning as a left-to-right shunt with a step-up in oxygen saturation at that level. This was corrected by operation on the twenty-ninth postoperative day and, 9 months after operation, the patient remains asymptomatic and has normal exercise tolerance.", "contents": "Transmyocardial left-to-right shunt complicating acute inferior wall myocardial infarction after aortocoronary bypass. A 61-year-old woman suffered an inferior wall myocardial infarction on the third day following aortocoronary bypass surgery. Her condition subsequently deteriorated and she was not responsive to vigorous conventional therapy. On the twenty-first postoperative day, selective coronary arteriography revealed a patent circumflex bypass graft and an occluded right coronary artery bypass graft. Left ventricular injection showed slight dilation of the ventricle and a reduced ejection fraction. A dissection from the left ventricular cavity into the myocardium, across the septum near the apex of the left ventricle with rupture into the right ventricle, was seen functioning as a left-to-right shunt with a step-up in oxygen saturation at that level. This was corrected by operation on the twenty-ninth postoperative day and, 9 months after operation, the patient remains asymptomatic and has normal exercise tolerance."}
{"id": "PMID:1079393", "title": "Investigating the child with frequent infections.", "content": "Children frequently present with a history of repeated infections. When these infections are serious and respond poorly to normally successful treatment programs, suspicion of a basic defect in one or more host defense mechanisms may be raised. In such cases defects in humoral or cell-mediated immunity, phagocytic cell functions or the complement system must be sought. Much of our knowledge about the development and functions of these cooperating but independent systems allows clinical observations to provide a generally accurate prediction of the nature of the defect involved. The degree of sophistication with which the systems can be evaluated and the possibilities for therapeutic manipulation are rapidly increasing. In this article are outlined the basic pathophysiological mechanisms needed to approach these increasingly recognized problems.", "contents": "Investigating the child with frequent infections. Children frequently present with a history of repeated infections. When these infections are serious and respond poorly to normally successful treatment programs, suspicion of a basic defect in one or more host defense mechanisms may be raised. In such cases defects in humoral or cell-mediated immunity, phagocytic cell functions or the complement system must be sought. Much of our knowledge about the development and functions of these cooperating but independent systems allows clinical observations to provide a generally accurate prediction of the nature of the defect involved. The degree of sophistication with which the systems can be evaluated and the possibilities for therapeutic manipulation are rapidly increasing. In this article are outlined the basic pathophysiological mechanisms needed to approach these increasingly recognized problems."}
{"id": "PMID:1079394", "title": "[Indication for coronary surgery with special reference to hemodynamic results].", "content": "An analysis is made whether and how far the aims of coronary artery bypass surgery improvement or relief of angina, improvement of the physical working capacity, improvement of the oxygen supply of ischemic myocardium, improvement of the myocardial function, improvement of longevity, can be attained. 48 percent of 87 patients had complete subsidance of angina pectoris after operation. There was a considerable improvement in 32 percent, no change in 14 percent, and a deterioration in 6 percent. After operation, the working capacity was improved in 73 percent of our patients significantly, in some cases nearly unlimited. By a comparison between loss of complaints and bypass function (open or closed) we found a good correlation between functioning bypasses and complaints. But there are some cases without complaints in spite of a closure of the bypass. Also the reverse, unchanged complaints in spite of an open bypass, was seen. A normalisation of preoperative disturbed myocardial function can be attained. Significant improvements, but also deteriorations can be found. Generally, there is a good correlation between improved myocardial function and improvement of the working capacity. Yet there is not rarely a discrepancy between a significant improvement of the working capacity and the left ventricular function. The explanation may be a loss or a postoperative augmented threshold for anging pectoris. Comparing the groups of patients with conservative and surgical treatment, the superiority of surgical treatment cannot be proved as yet. An exception seems to be the stenosis of the left main coronary artery. With this exception, prognostic considerations cannot play a role for the indication of a coronary bypass operation. Aspects for the indication of coronary surgery are untractable or disabling angina pectoris and the consideration how impending complications can be prevented.", "contents": "[Indication for coronary surgery with special reference to hemodynamic results]. An analysis is made whether and how far the aims of coronary artery bypass surgery improvement or relief of angina, improvement of the physical working capacity, improvement of the oxygen supply of ischemic myocardium, improvement of the myocardial function, improvement of longevity, can be attained. 48 percent of 87 patients had complete subsidance of angina pectoris after operation. There was a considerable improvement in 32 percent, no change in 14 percent, and a deterioration in 6 percent. After operation, the working capacity was improved in 73 percent of our patients significantly, in some cases nearly unlimited. By a comparison between loss of complaints and bypass function (open or closed) we found a good correlation between functioning bypasses and complaints. But there are some cases without complaints in spite of a closure of the bypass. Also the reverse, unchanged complaints in spite of an open bypass, was seen. A normalisation of preoperative disturbed myocardial function can be attained. Significant improvements, but also deteriorations can be found. Generally, there is a good correlation between improved myocardial function and improvement of the working capacity. Yet there is not rarely a discrepancy between a significant improvement of the working capacity and the left ventricular function. The explanation may be a loss or a postoperative augmented threshold for anging pectoris. Comparing the groups of patients with conservative and surgical treatment, the superiority of surgical treatment cannot be proved as yet. An exception seems to be the stenosis of the left main coronary artery. With this exception, prognostic considerations cannot play a role for the indication of a coronary bypass operation. Aspects for the indication of coronary surgery are untractable or disabling angina pectoris and the consideration how impending complications can be prevented."}
{"id": "PMID:1079396", "title": "Relation of intraoperative or early postoperative transmural myocardial infarction to patency of aortocoronary bypass grafts and to diseased ungrafted coronary arteries.", "content": "Serial preoperative and postoperative electrocardiograms and vectorcardiograms were obtained in 500 patients undergoing saphenous vein aortocoronary artery bypass graft surgery. Evidence of transmural myocardial infarction was found early postoperatively in 67 patients (13 percent). Age and sex distributions, number of vessels diseased or vessels grafted, and preoperative and postoperative New York Heart Association functional classification (mean follow-up, 26 months) did not differ in the groups with and without infarction. Increased duration of cardiopulmonary bypass time (more than 120 minutes) was slightly greater in the group with infarction (P smaller than 0.05). Multivariate analysis revealed that 60 percent of patients in the group with infarction were identified by a 1st day serum glutamic oxaloacetic transaminase value greater than 100 U/liter; however, for each such patient identified, there was approximately one false positive result. Use of other values (creatine phosphokinase, cardiopulmonary bypass time and total anoxic rest time) did not improve discrimination. Twenty-five percent of all transmural infarctions occurred within the zone of myocardium supplied by a diseased ungrafted artery. In 32 patients with early evidence of transmural mycardial infarction in a zone of myocardium supplied by a grafted artery, postoperative angiography showed as many with patent as with occluded grafts. Of 154 patients in the group without infarction who had early postoperative graft angiograms, 30 (19 percent) had one graft occluded and yet no evidence of transmural infarction by our criteria. Therefore, early postoperative evidence of transmural myocardial infarction as defined in this study is an unreliable indicator of the status of the graft supplying the zone of infarction.", "contents": "Relation of intraoperative or early postoperative transmural myocardial infarction to patency of aortocoronary bypass grafts and to diseased ungrafted coronary arteries. Serial preoperative and postoperative electrocardiograms and vectorcardiograms were obtained in 500 patients undergoing saphenous vein aortocoronary artery bypass graft surgery. Evidence of transmural myocardial infarction was found early postoperatively in 67 patients (13 percent). Age and sex distributions, number of vessels diseased or vessels grafted, and preoperative and postoperative New York Heart Association functional classification (mean follow-up, 26 months) did not differ in the groups with and without infarction. Increased duration of cardiopulmonary bypass time (more than 120 minutes) was slightly greater in the group with infarction (P smaller than 0.05). Multivariate analysis revealed that 60 percent of patients in the group with infarction were identified by a 1st day serum glutamic oxaloacetic transaminase value greater than 100 U/liter; however, for each such patient identified, there was approximately one false positive result. Use of other values (creatine phosphokinase, cardiopulmonary bypass time and total anoxic rest time) did not improve discrimination. Twenty-five percent of all transmural infarctions occurred within the zone of myocardium supplied by a diseased ungrafted artery. In 32 patients with early evidence of transmural mycardial infarction in a zone of myocardium supplied by a grafted artery, postoperative angiography showed as many with patent as with occluded grafts. Of 154 patients in the group without infarction who had early postoperative graft angiograms, 30 (19 percent) had one graft occluded and yet no evidence of transmural infarction by our criteria. Therefore, early postoperative evidence of transmural myocardial infarction as defined in this study is an unreliable indicator of the status of the graft supplying the zone of infarction."}
{"id": "PMID:1079397", "title": "Aortocoronary bypass in the diabetic patient.", "content": "ONe hundred twelve patients undergoing aortocoronary bypass--35 with diabetes of adult onset and 77 without diabetes--were studied to determine whether diabetic patients have additional operative risks and greater operative mortality and whether their coronary disease differs from that of nondiabetic patients. Among the diabetic patients there was a greater prevalence of preoperative unstable angina, prior myocardial infarction and class IV functional disability (New York Heart Association criteria). The major coronary arteries angiographically and at operation appeared similar in both groups. The blood flow rates measured in aortocoronary bypass vein grafts were similar in both groups, raising doubt about the presence of microvascular disease in the myocardium of the diabetic patient. Preliminary follow-up results demonstrated relief of anginal symptoms in 76 percent of diabetic and 78 percent of nondiabetic patients. The operative mortality rate of 9 percent in diabetic and 4 percent in nondiabetic patients occurred among the first 40 patients in the series; no patient in either group has died in the immediate postoperative period during the last 18 months of the study. Aortocoronary bypass should be recommended to diabetic patients with symptomatic coronary arteriosclerosis using the same criteria for operability applied to the nondiabetic population.", "contents": "Aortocoronary bypass in the diabetic patient. ONe hundred twelve patients undergoing aortocoronary bypass--35 with diabetes of adult onset and 77 without diabetes--were studied to determine whether diabetic patients have additional operative risks and greater operative mortality and whether their coronary disease differs from that of nondiabetic patients. Among the diabetic patients there was a greater prevalence of preoperative unstable angina, prior myocardial infarction and class IV functional disability (New York Heart Association criteria). The major coronary arteries angiographically and at operation appeared similar in both groups. The blood flow rates measured in aortocoronary bypass vein grafts were similar in both groups, raising doubt about the presence of microvascular disease in the myocardium of the diabetic patient. Preliminary follow-up results demonstrated relief of anginal symptoms in 76 percent of diabetic and 78 percent of nondiabetic patients. The operative mortality rate of 9 percent in diabetic and 4 percent in nondiabetic patients occurred among the first 40 patients in the series; no patient in either group has died in the immediate postoperative period during the last 18 months of the study. Aortocoronary bypass should be recommended to diabetic patients with symptomatic coronary arteriosclerosis using the same criteria for operability applied to the nondiabetic population."}
{"id": "PMID:1079398", "title": "Surgical versus medical treatment of occlusive disease confined to the left anterior descending coronary artery.", "content": "The results of saphenous vein bypass grafting and medical treatment were compared in 53 patients with stable angina pectoris, high grade occlusive disease confined to the left anterior descending coronary artery and normal or minimally impaired left ventricular function. Survival, incidence of myocardial infarction, relief of angina and response to exercise testing were evaluated. In the 29 surgically treated patients, followed up a mean of 24 months, there were two late deaths (7 percent) and five myocardial infarctions (17 percent). Twelve patients (41 percent) were free of angina and the majority had increased exercise performance when tested up to 18 months postoperatively. In the 24 medically treated patients, there were no deaths and one myocardial infarction (4 percent) in a mean follow-up period of 37 months. Six patients (25 percent) were free of angina. Less improvement in exercise performance was observed than in the surgically treated group. This subset of patients with isolated left anterior descending coronary artery disease has a favorable prognosis that is not enhanced by bypass grafting. Surgical treatment is more effective than medical treatment in relieving angina and improving exercise performance in the early years after coronary arteriography.", "contents": "Surgical versus medical treatment of occlusive disease confined to the left anterior descending coronary artery. The results of saphenous vein bypass grafting and medical treatment were compared in 53 patients with stable angina pectoris, high grade occlusive disease confined to the left anterior descending coronary artery and normal or minimally impaired left ventricular function. Survival, incidence of myocardial infarction, relief of angina and response to exercise testing were evaluated. In the 29 surgically treated patients, followed up a mean of 24 months, there were two late deaths (7 percent) and five myocardial infarctions (17 percent). Twelve patients (41 percent) were free of angina and the majority had increased exercise performance when tested up to 18 months postoperatively. In the 24 medically treated patients, there were no deaths and one myocardial infarction (4 percent) in a mean follow-up period of 37 months. Six patients (25 percent) were free of angina. Less improvement in exercise performance was observed than in the surgically treated group. This subset of patients with isolated left anterior descending coronary artery disease has a favorable prognosis that is not enhanced by bypass grafting. Surgical treatment is more effective than medical treatment in relieving angina and improving exercise performance in the early years after coronary arteriography."}
{"id": "PMID:1079399", "title": "Cellular recognition and activation within the lymphoid system.", "content": "The present study is concerned with cellular recognition and activation within the lymphoid system. Data presented indicate that in human peripheral blood phagocytes recognize and preferentially bind phagocytes, and thymus-derived (T) lymphocytes recognize and preferentially bind other T lymphocytes rather than thymus-indepedent (B) lymphocytes. Recognition of non-self results in activation of lymphoid cells; recent data using a calcium ionophore, A23 187, suggest that calcium may act as an intracellular messenger by which signals for cellular activation are transmitted from the cell surface to the nucleus.", "contents": "Cellular recognition and activation within the lymphoid system. The present study is concerned with cellular recognition and activation within the lymphoid system. Data presented indicate that in human peripheral blood phagocytes recognize and preferentially bind phagocytes, and thymus-derived (T) lymphocytes recognize and preferentially bind other T lymphocytes rather than thymus-indepedent (B) lymphocytes. Recognition of non-self results in activation of lymphoid cells; recent data using a calcium ionophore, A23 187, suggest that calcium may act as an intracellular messenger by which signals for cellular activation are transmitted from the cell surface to the nucleus."}
{"id": "PMID:1079400", "title": "Neonatal Haemophilus influenzae infection. Report of eight cases and review of the literature.", "content": "Eight cases of neonatal Haemophilus influenzae infection occurred at Colorado General Hospital during a 3 1/2-year period. Four of the cases were due to nontypable organisms and four to type b organisms. Four were bacteremic, only two of which were type b. None of the patients had meningitis. In all instances, maternal genital tract cultures yielded H infleuzae that, when tested, was of the same serotype as the infant isolate. A survey of cervical cultures among normal and pregnant women yielded H influenzae in less than 1%. There are several possible reasons for the disparity between the apparent high incidence of neonatal infection and low prevalence of maternal carriage.", "contents": "Neonatal Haemophilus influenzae infection. Report of eight cases and review of the literature. Eight cases of neonatal Haemophilus influenzae infection occurred at Colorado General Hospital during a 3 1/2-year period. Four of the cases were due to nontypable organisms and four to type b organisms. Four were bacteremic, only two of which were type b. None of the patients had meningitis. In all instances, maternal genital tract cultures yielded H infleuzae that, when tested, was of the same serotype as the infant isolate. A survey of cervical cultures among normal and pregnant women yielded H influenzae in less than 1%. There are several possible reasons for the disparity between the apparent high incidence of neonatal infection and low prevalence of maternal carriage."}
{"id": "PMID:1079404", "title": "Hereditary telangiectasia manifested as gastrointestinal bleeding without external visible telangiectasia.", "content": "Gastrointestinal bleeding is one of the most common problems confronting the physician. In most instances, the source of the bleeding is easily identified, e.g., peptic ulcer disease, bleeding esophageal varices or a colonic lesion. Recurrent gastrointestinal bleeding, however, represents one of the major enigmas confronting the practicing physician. The patient experiences intermittent episodes of weakness, easy fatigability and anemia with occult blood in the stool. Multiple barium radiographic examinations and often endoscopy and exploratory laparotomy are unrevealing as to the etiology of the bleeding. We have had occasion to investigate three such patients. Multiple evaluations of these individuals did not reveal the source of the bleeding until many years later--Rendu-Osler-Weber Disease (ROW). At the time of their evaluations these patients did not reveal the usual telangiectatic areas present on the skin and oral mucous membrane. The diagnosis was suggested by the presence of multiple gastric mucosal telangiectasia identified by gastroscopic examination. This report emphasizes the need for thorough endoscopic evaluation of patients with gastrointestinal bleeding and describes the gastroscopic findings that indicated hereditary telangiectasia as the probable source of bleeding from the gastrointestinal tract.", "contents": "Hereditary telangiectasia manifested as gastrointestinal bleeding without external visible telangiectasia. Gastrointestinal bleeding is one of the most common problems confronting the physician. In most instances, the source of the bleeding is easily identified, e.g., peptic ulcer disease, bleeding esophageal varices or a colonic lesion. Recurrent gastrointestinal bleeding, however, represents one of the major enigmas confronting the practicing physician. The patient experiences intermittent episodes of weakness, easy fatigability and anemia with occult blood in the stool. Multiple barium radiographic examinations and often endoscopy and exploratory laparotomy are unrevealing as to the etiology of the bleeding. We have had occasion to investigate three such patients. Multiple evaluations of these individuals did not reveal the source of the bleeding until many years later--Rendu-Osler-Weber Disease (ROW). At the time of their evaluations these patients did not reveal the usual telangiectatic areas present on the skin and oral mucous membrane. The diagnosis was suggested by the presence of multiple gastric mucosal telangiectasia identified by gastroscopic examination. This report emphasizes the need for thorough endoscopic evaluation of patients with gastrointestinal bleeding and describes the gastroscopic findings that indicated hereditary telangiectasia as the probable source of bleeding from the gastrointestinal tract."}
{"id": "PMID:1079406", "title": "Color vision testing in young children: a review.", "content": "It is often recommended that children be screened for possible color vision deficiencies as early as possible. This paper examines the validity of commercially-available color vision tests when used with young children (age three to seven years). It is concluded that with the possible exception of the anomaloscope, no test is entirely suited for children, in most cases because the test makes cognitive demands beyond the capability of the young child.", "contents": "Color vision testing in young children: a review. It is often recommended that children be screened for possible color vision deficiencies as early as possible. This paper examines the validity of commercially-available color vision tests when used with young children (age three to seven years). It is concluded that with the possible exception of the anomaloscope, no test is entirely suited for children, in most cases because the test makes cognitive demands beyond the capability of the young child."}
{"id": "PMID:1079407", "title": "The Titmus Pediatric Color Perception Test as a color vision screener: a comparative study.", "content": "The Titmus Pediatric Color Perception Test was compared to three other standard tests of color vision (Ishihara, Dvorine, Farnsworth D-15) with a population of adults. It is concluded that the results of the Titmus test are consistent with those of the other tests if certain specific scoring methods, described in the text, are used.", "contents": "The Titmus Pediatric Color Perception Test as a color vision screener: a comparative study. The Titmus Pediatric Color Perception Test was compared to three other standard tests of color vision (Ishihara, Dvorine, Farnsworth D-15) with a population of adults. It is concluded that the results of the Titmus test are consistent with those of the other tests if certain specific scoring methods, described in the text, are used."}
{"id": "PMID:1079405", "title": "Prolonged bleeding times with factor IX and XI deficiency von Willebrand's syndromes.", "content": "This report describes three patients with a prolonged bleeding time and reduced platelet retention on glass beads, two of the three established criteria for conventional von Willebrand's disease (vWd). However, the third standard, the diminution of factor VIII, was replaced by a decrease of factor IX or XI in the first two instances, respectively, and combined factor VII and IX deficiency was present in the last patient. These associations suggest that the vW (bleeding time/platelet retention) factor is not necessarily homogeneous, may not be dependent on a single genetic determinant, and may be present in a molecular complex composed not only of factor VIII but of other blood clotting proteins as well. Although simulating classic vW, these homologues may more appropriately be called von Willebrand syndromes. Finally, unusual as they may be, their clinical recognition is important to insure that such patients receive not only factor VIII concentrates in treatment since these materials do not contain factors IX and XI and may not even be a suitable source of platelet retention factor under these modified circumstances.", "contents": "Prolonged bleeding times with factor IX and XI deficiency von Willebrand's syndromes. This report describes three patients with a prolonged bleeding time and reduced platelet retention on glass beads, two of the three established criteria for conventional von Willebrand's disease (vWd). However, the third standard, the diminution of factor VIII, was replaced by a decrease of factor IX or XI in the first two instances, respectively, and combined factor VII and IX deficiency was present in the last patient. These associations suggest that the vW (bleeding time/platelet retention) factor is not necessarily homogeneous, may not be dependent on a single genetic determinant, and may be present in a molecular complex composed not only of factor VIII but of other blood clotting proteins as well. Although simulating classic vW, these homologues may more appropriately be called von Willebrand syndromes. Finally, unusual as they may be, their clinical recognition is important to insure that such patients receive not only factor VIII concentrates in treatment since these materials do not contain factors IX and XI and may not even be a suitable source of platelet retention factor under these modified circumstances."}
{"id": "PMID:1079408", "title": "Abnormal left renal vein. No barrier to Warren shunt.", "content": "The purpose of the distal splenorenal shunt is to provide decompression of gastroesophageal varices while avoiding insult to the diseased liver. However, the technical difficulty of the operation has prevented widespread application of the procedure. It is important, therefore, that the technical problems an hazards of the operation continue to undergo analysis. This report has described successful establishment of a Warren shunt in three patients with abnormalities of the left renal vein. Previous left nephrectomy or an anomaly of the left renal vein does not preclude the use of the distal splenorenal shunt in the treatment of variceal hemorrhage.", "contents": "Abnormal left renal vein. No barrier to Warren shunt. The purpose of the distal splenorenal shunt is to provide decompression of gastroesophageal varices while avoiding insult to the diseased liver. However, the technical difficulty of the operation has prevented widespread application of the procedure. It is important, therefore, that the technical problems an hazards of the operation continue to undergo analysis. This report has described successful establishment of a Warren shunt in three patients with abnormalities of the left renal vein. Previous left nephrectomy or an anomaly of the left renal vein does not preclude the use of the distal splenorenal shunt in the treatment of variceal hemorrhage."}
{"id": "PMID:1079409", "title": "Fiberoptic endoscopy. Precise definition of upper gastrointestinal disease.", "content": "When properly employed, endoscopic examination of the upper gastrointestinal tract with the flexible maneuverable-tip fiberoptic instruments is the most accurate method of diagnosing any upper gastrointestinal tract disease. Two hundred consecutive endoscopic procedures in the upper gastrointestinal tract were performed without significant morbidity or mortality; the results were reviewed to ascertain the overall diagnostic value of this modality. Although the overall numbers in each subgroup are still small, the experience indicates that: (1) thorough endoscopic examination of the upper gastrointestinal tract can be carried out expeditiously in most patients without morbidity; (2) upper gastrointestinal tract disease can be precisely defined in the majority of patients; and (3) endoscopic examination frequently alters the initial clinical diagnosis. The precise cause of upper gastrointestinal tract hemorrhage can be diagnosed in at least three of four cases. Some unnecessary operations can be avoided and proper therapy for specific sources of bleeding can be initiated promptly.", "contents": "Fiberoptic endoscopy. Precise definition of upper gastrointestinal disease. When properly employed, endoscopic examination of the upper gastrointestinal tract with the flexible maneuverable-tip fiberoptic instruments is the most accurate method of diagnosing any upper gastrointestinal tract disease. Two hundred consecutive endoscopic procedures in the upper gastrointestinal tract were performed without significant morbidity or mortality; the results were reviewed to ascertain the overall diagnostic value of this modality. Although the overall numbers in each subgroup are still small, the experience indicates that: (1) thorough endoscopic examination of the upper gastrointestinal tract can be carried out expeditiously in most patients without morbidity; (2) upper gastrointestinal tract disease can be precisely defined in the majority of patients; and (3) endoscopic examination frequently alters the initial clinical diagnosis. The precise cause of upper gastrointestinal tract hemorrhage can be diagnosed in at least three of four cases. Some unnecessary operations can be avoided and proper therapy for specific sources of bleeding can be initiated promptly."}
{"id": "PMID:1079410", "title": "Meckel's diverticulum: should it be excised incidentally at operation?", "content": "Meckel's diverticulum is the most common congenital abnormality of the small intestine, occurring in about 2 per cent of the population. Complications of a Meckel's diverticulum, including hemorrhage, inflammation, bowel obstruction, and neoplasms, occur in 15 to 33 per cent of cases and invariably demand operative treatment. When signs or symptoms arise from a Meckel's diverticulum, morbidity and mortality are high. Incidental Meckel's diverticulectomy should be performed at any age when the morbidity and mortality of the primary procedure will not be increased substantially.", "contents": "Meckel's diverticulum: should it be excised incidentally at operation? Meckel's diverticulum is the most common congenital abnormality of the small intestine, occurring in about 2 per cent of the population. Complications of a Meckel's diverticulum, including hemorrhage, inflammation, bowel obstruction, and neoplasms, occur in 15 to 33 per cent of cases and invariably demand operative treatment. When signs or symptoms arise from a Meckel's diverticulum, morbidity and mortality are high. Incidental Meckel's diverticulectomy should be performed at any age when the morbidity and mortality of the primary procedure will not be increased substantially."}
{"id": "PMID:1079411", "title": "Pancreatic pseudocyst with hemorrhage into the gastrointestinal tract through the duct of Wirsung.", "content": "A case of massive upper gastrointestinal hemorrhage is presented in which a pancreatic pseudocyst eroded into the splenic artery resulting in intracystic hemorrhage through the duct of Wirsung. Total excision of the pseudocyst, spleen, and tail of the pancreas is recommended.", "contents": "Pancreatic pseudocyst with hemorrhage into the gastrointestinal tract through the duct of Wirsung. A case of massive upper gastrointestinal hemorrhage is presented in which a pancreatic pseudocyst eroded into the splenic artery resulting in intracystic hemorrhage through the duct of Wirsung. Total excision of the pseudocyst, spleen, and tail of the pancreas is recommended."}
{"id": "PMID:1079412", "title": "Treatment of Eales' disease with photocoagulation.", "content": "224 eyes with Eales' disease were subjected to photocoagulation. In 205 (=91%) the disease could be brought to a morphological standstill over a mean observation time of 5 years after completion of treatment. Visual acuity remained unchanged or improved in 185 eyes (=87%). Postcoagulative macular puckering occured in 6 eyes (=3%).", "contents": "Treatment of Eales' disease with photocoagulation. 224 eyes with Eales' disease were subjected to photocoagulation. In 205 (=91%) the disease could be brought to a morphological standstill over a mean observation time of 5 years after completion of treatment. Visual acuity remained unchanged or improved in 185 eyes (=87%). Postcoagulative macular puckering occured in 6 eyes (=3%)."}
{"id": "PMID:1079413", "title": "Ultrastructural changes of the capillaries of the cat iris in experimental neuroparalytic keratitis.", "content": "In order to study the morphological basis of the increased permeability of the capillaries of the iris in neuroparalytic keratitis the ophthalmic division of the trigeminal nerve in the cat was denervated using a stereotactic method. The homolateral iris was studied by electron microscopy three days after denervation. Abnormally large pinocytotic vacuoles were observed in the endothelial cells of the iris capillaries and the intercellular junctions of the endothelial cells showed widened inter-cellular space and macula occludens. These ultrastructural changes may explain the protein leakage into the anterior chamber in neuroparalytic keratitis.", "contents": "Ultrastructural changes of the capillaries of the cat iris in experimental neuroparalytic keratitis. In order to study the morphological basis of the increased permeability of the capillaries of the iris in neuroparalytic keratitis the ophthalmic division of the trigeminal nerve in the cat was denervated using a stereotactic method. The homolateral iris was studied by electron microscopy three days after denervation. Abnormally large pinocytotic vacuoles were observed in the endothelial cells of the iris capillaries and the intercellular junctions of the endothelial cells showed widened inter-cellular space and macula occludens. These ultrastructural changes may explain the protein leakage into the anterior chamber in neuroparalytic keratitis."}
{"id": "PMID:1079414", "title": "[Objective determination of accommodation in eyes with permanent central scotoma due to amblyopia (author's transl)].", "content": "Objective determination of accommodation in 38 children with permanent central scotoma due to amblyopia on one side (with central and eccentric fixation) revealed: 1. The physiological adaptation of refraction for far and near distances did not serve its purpose. 2. There was either no or an irregular response on hyperopia, which was produced by placing concave lenses in front of the eye while fixing a far object, or on myopia produced by placing convex lenses in front of the eye while fixing a near object. 3. On hindering clear vision which could not be compensated by accommodation there was contrary to normal eyes no reactive change of refraction at all or only a poor one. The accommodation behavior in eyes with permanent central scotoma due to amblyopia does not differ from that in eyes with permanent central scotoma due to organic lesions when using the same test methods.", "contents": "[Objective determination of accommodation in eyes with permanent central scotoma due to amblyopia (author's transl)]. Objective determination of accommodation in 38 children with permanent central scotoma due to amblyopia on one side (with central and eccentric fixation) revealed: 1. The physiological adaptation of refraction for far and near distances did not serve its purpose. 2. There was either no or an irregular response on hyperopia, which was produced by placing concave lenses in front of the eye while fixing a far object, or on myopia produced by placing convex lenses in front of the eye while fixing a near object. 3. On hindering clear vision which could not be compensated by accommodation there was contrary to normal eyes no reactive change of refraction at all or only a poor one. The accommodation behavior in eyes with permanent central scotoma due to amblyopia does not differ from that in eyes with permanent central scotoma due to organic lesions when using the same test methods."}
{"id": "PMID:1079415", "title": "The influence of moistening fluids on the measuring results of applanations-tonometry (author's transl).", "content": "The applanation tonometry is influenced by several factors not considered by the law of Imbert-Fick. For instance, a change in the conditions of moistening the cornea may falsify the result of measuring. This has been proved clinically and experimentally with Polyvinylpyrrolidon. The fault in measuring is larger with applanation tonometers producing variable applanation areas along with constant pressure than with those flattening small invariable corneal area. Aside of the difficulty of exact measurement of the applanation areas the latter tonometers are superior to the former ones because of this reason, too.", "contents": "The influence of moistening fluids on the measuring results of applanations-tonometry (author's transl). The applanation tonometry is influenced by several factors not considered by the law of Imbert-Fick. For instance, a change in the conditions of moistening the cornea may falsify the result of measuring. This has been proved clinically and experimentally with Polyvinylpyrrolidon. The fault in measuring is larger with applanation tonometers producing variable applanation areas along with constant pressure than with those flattening small invariable corneal area. Aside of the difficulty of exact measurement of the applanation areas the latter tonometers are superior to the former ones because of this reason, too."}
{"id": "PMID:1079416", "title": "[The influence of the eye prominence on the EOG potential level (author's transl)].", "content": "The influence of globe prominence on the electro-oculogram potential level was measured in patients with unilateral protrusio bulbi, myopia or hyperopia. The EOG potential level on the side with the more prominent globe was lower if the prominence was due to protrusion. If the prominence was due to myopia, the EOG potential was higher compared to the contralateral non-myopic eye. In hyperopic eyes the potential level was lower.", "contents": "[The influence of the eye prominence on the EOG potential level (author's transl)]. The influence of globe prominence on the electro-oculogram potential level was measured in patients with unilateral protrusio bulbi, myopia or hyperopia. The EOG potential level on the side with the more prominent globe was lower if the prominence was due to protrusion. If the prominence was due to myopia, the EOG potential was higher compared to the contralateral non-myopic eye. In hyperopic eyes the potential level was lower."}
{"id": "PMID:1079417", "title": "Fluorescein in the human optic disc,.", "content": "1. Compressing the globe to intraocular pressure values even higher than the systolic ophthalmica pressure does not totally empty the capillaries of the disc. 2. Almost only the plasma layer at the wall of the retinal and papillary vessels is the source of vessel fluorescence seen after intravenous fluorescein injection. Therefore small vessels fluoresce almost as much as big ones and extravasates appear as very brilliant patches. 3. Fluorescein diffuses into the disc tissue mostly from the surrounding tissues (uvea, ev. sclera). 4. The blood circulation in the disc diminishes more than that of the retinal vessels if the eye is compressed, 5. Fluorescein is not especially appropriate for the measurement of the circulation in the disc.", "contents": "Fluorescein in the human optic disc,. 1. Compressing the globe to intraocular pressure values even higher than the systolic ophthalmica pressure does not totally empty the capillaries of the disc. 2. Almost only the plasma layer at the wall of the retinal and papillary vessels is the source of vessel fluorescence seen after intravenous fluorescein injection. Therefore small vessels fluoresce almost as much as big ones and extravasates appear as very brilliant patches. 3. Fluorescein diffuses into the disc tissue mostly from the surrounding tissues (uvea, ev. sclera). 4. The blood circulation in the disc diminishes more than that of the retinal vessels if the eye is compressed, 5. Fluorescein is not especially appropriate for the measurement of the circulation in the disc."}
{"id": "PMID:1079418", "title": "Retinal pigment epithelial degeneration, partial retinal atrophy and macular hole in acute lymphocytic leukemia.", "content": "A seven year old child with acute lymphocytic leukemia presented at autopsy with a macular hole, and degeneration of the retinal pigment epithelium and its overlying photoreceptors. She had been treated with cobalt 60, methotrexate, vincristine cyclophosphamide and prednisone. A review of the literature of similar findings as well as pigment epithelial degenerations from other causes is presented.", "contents": "Retinal pigment epithelial degeneration, partial retinal atrophy and macular hole in acute lymphocytic leukemia. A seven year old child with acute lymphocytic leukemia presented at autopsy with a macular hole, and degeneration of the retinal pigment epithelium and its overlying photoreceptors. She had been treated with cobalt 60, methotrexate, vincristine cyclophosphamide and prednisone. A review of the literature of similar findings as well as pigment epithelial degenerations from other causes is presented."}
{"id": "PMID:1079419", "title": "The significance of tears' lactate dehydrogenase in health, and external eye diseases.", "content": "The normal values of the lactate dehydrogenase contents of tears in the presence of pyruvate and lactate as substrates were determined. The quotients of the LDH values measured in the presence of the two different substrates, and also the ratio of the subunits (H and M) of the isoenzymes, differ from the corresponding data of blood serum. In the event of external inflammatory diseases of the eye, LDHp/LDHl decreases in the tears; this deviation is enhanced by treatment with drugs inhibiting protein synthesis, primarily iododeoxyuridine. Conversely, in trachoma the ratio of the LDH isoenzymes containing mainly M (muscle) subunits are increasing. In diabetes mellitus the shift of metabolism toward the citrate cycle is reflected to a larger extent by the LDH values in the blood serum than by those in tears.", "contents": "The significance of tears' lactate dehydrogenase in health, and external eye diseases. The normal values of the lactate dehydrogenase contents of tears in the presence of pyruvate and lactate as substrates were determined. The quotients of the LDH values measured in the presence of the two different substrates, and also the ratio of the subunits (H and M) of the isoenzymes, differ from the corresponding data of blood serum. In the event of external inflammatory diseases of the eye, LDHp/LDHl decreases in the tears; this deviation is enhanced by treatment with drugs inhibiting protein synthesis, primarily iododeoxyuridine. Conversely, in trachoma the ratio of the LDH isoenzymes containing mainly M (muscle) subunits are increasing. In diabetes mellitus the shift of metabolism toward the citrate cycle is reflected to a larger extent by the LDH values in the blood serum than by those in tears."}
{"id": "PMID:1079420", "title": "[The influence of riboflavin on vitreous homogenate (author's transl)].", "content": "Sunlight causes a decrease of viscosity of a mixture of riboflavin and ox vitreous homogenate while without riboflavin no reaction can be observed. The mechanism of this reaction is not yet clarified. It is possible that the reaction is closely related with the degradation of the viscosity of a hyaluronic acid solution by ascorbic acid cause of the production of H2O2 in both reactions. As an other mechanism the transfer of light energy on hyaluronic acid by riboflavin can be assumed.", "contents": "[The influence of riboflavin on vitreous homogenate (author's transl)]. Sunlight causes a decrease of viscosity of a mixture of riboflavin and ox vitreous homogenate while without riboflavin no reaction can be observed. The mechanism of this reaction is not yet clarified. It is possible that the reaction is closely related with the degradation of the viscosity of a hyaluronic acid solution by ascorbic acid cause of the production of H2O2 in both reactions. As an other mechanism the transfer of light energy on hyaluronic acid by riboflavin can be assumed."}
{"id": "PMID:1079421", "title": "[The diagnosis of pseudotumours of the macula by means of ultrasonography (author's transl)].", "content": "The echograms of 35 patients affected with pseudotumours of the macula were analysed in detail. Pseudotumours and malignant melanomas can be distinguished by means of ultrasound with high probability if the minimal prominence of the tumour is not lower than 1.1 mm, and with certainty if the minimal prominence is not less than 1.5 mm. The echogram of pseudotumours is mainly characterized by its extraordinary irregularity.", "contents": "[The diagnosis of pseudotumours of the macula by means of ultrasonography (author's transl)]. The echograms of 35 patients affected with pseudotumours of the macula were analysed in detail. Pseudotumours and malignant melanomas can be distinguished by means of ultrasound with high probability if the minimal prominence of the tumour is not lower than 1.1 mm, and with certainty if the minimal prominence is not less than 1.5 mm. The echogram of pseudotumours is mainly characterized by its extraordinary irregularity."}
{"id": "PMID:1079422", "title": "Protease inhibitors in chronic obstructive pulmonary disease.", "content": "Quantification of the major plasma protease inhibitors and genetic typing of alpha1-antirypsin were done in 107 patients with chronic obstructive pulmonary disease and in 91 control subjects with normal ventilatory function who were similar with respect to age, race, and sex. There was a significant increase in frequency of the PiZ gene and the Pi MZ phenotype of alpha1-antitrypsin among the patients when compared with the control subjects. No evidence for a primary deficiency of any other antiprotease was found; however, the mean concentration of inter-alpha-trypsin inhibitor was significantly lower in the patients than the control subjects, and moderate deficiency of alpha1-antichymotrypsin was noted in a few patients. These data indicate an increased risk of developing chronic obstructive pulmonary disease in persons with the Pi MZ phenotype of alpha1-antirypsin and suggest a possible relationship between these diseases and low serum concentrations of inter-alpha-trypsin inhibitor.", "contents": "Protease inhibitors in chronic obstructive pulmonary disease. Quantification of the major plasma protease inhibitors and genetic typing of alpha1-antirypsin were done in 107 patients with chronic obstructive pulmonary disease and in 91 control subjects with normal ventilatory function who were similar with respect to age, race, and sex. There was a significant increase in frequency of the PiZ gene and the Pi MZ phenotype of alpha1-antitrypsin among the patients when compared with the control subjects. No evidence for a primary deficiency of any other antiprotease was found; however, the mean concentration of inter-alpha-trypsin inhibitor was significantly lower in the patients than the control subjects, and moderate deficiency of alpha1-antichymotrypsin was noted in a few patients. These data indicate an increased risk of developing chronic obstructive pulmonary disease in persons with the Pi MZ phenotype of alpha1-antirypsin and suggest a possible relationship between these diseases and low serum concentrations of inter-alpha-trypsin inhibitor."}
{"id": "PMID:1079423", "title": "Gastrointestinal bleeding: treatment with intra-arterial vasopressin.", "content": "Seventeen patients with upper and lower gastrointestinal hemorrhage were treated with selective intra-arterial continuous vasopressin infusion. 62.5 per cent of variceal and 60 per cent of nonvariceal episodes of hemorrhages were permanently controlled by vasopressin. The initial total success rate was 76.5 per cent. No major complications occurred. Early success with this technique has encouraged its continued use under a controlled setting for indications for its use to be evaluated.", "contents": "Gastrointestinal bleeding: treatment with intra-arterial vasopressin. Seventeen patients with upper and lower gastrointestinal hemorrhage were treated with selective intra-arterial continuous vasopressin infusion. 62.5 per cent of variceal and 60 per cent of nonvariceal episodes of hemorrhages were permanently controlled by vasopressin. The initial total success rate was 76.5 per cent. No major complications occurred. Early success with this technique has encouraged its continued use under a controlled setting for indications for its use to be evaluated."}
{"id": "PMID:1079426", "title": "The role of the thymus in spontaneous autoimmune thyroiditis.", "content": "In two models of genetically determined, spontaneous autoimmune thyroiditis, neonatal thymectomy increases the incidience and severity of disease. The simpliest explanation is that the thymus, or thymus-derived cells, normally exert a suppressive effect on the autoimmune response to thyroglobulin in addition to the helper function that is necessary to initiate the autoimmune reaction.", "contents": "The role of the thymus in spontaneous autoimmune thyroiditis. In two models of genetically determined, spontaneous autoimmune thyroiditis, neonatal thymectomy increases the incidience and severity of disease. The simpliest explanation is that the thymus, or thymus-derived cells, normally exert a suppressive effect on the autoimmune response to thyroglobulin in addition to the helper function that is necessary to initiate the autoimmune reaction."}
{"id": "PMID:1079430", "title": "The mitogenic activity of polyadenylic-polyuridyclic acid complexes.", "content": "Polyadenylic-polyuridylic acid complexes (poly A:U) at the 1-5 mu g level, were mitogenic for spleen cells when given intravenously to normal Balb or cortisone-treated mice. Similarly, mitogenicity was evident when poly A:U was added to tissue culture fluids containing spleen cells from normal or cortisone-treated mice, or bone marrow cells from normal mice. Under these conditions, this adjuvant was not mitogenic for thymus cells or mesenteric lymph node cells, either in vivo or in vitro.", "contents": "The mitogenic activity of polyadenylic-polyuridyclic acid complexes. Polyadenylic-polyuridylic acid complexes (poly A:U) at the 1-5 mu g level, were mitogenic for spleen cells when given intravenously to normal Balb or cortisone-treated mice. Similarly, mitogenicity was evident when poly A:U was added to tissue culture fluids containing spleen cells from normal or cortisone-treated mice, or bone marrow cells from normal mice. Under these conditions, this adjuvant was not mitogenic for thymus cells or mesenteric lymph node cells, either in vivo or in vitro."}
{"id": "PMID:1079439", "title": "Effect of fistulae on endolymphatic hydrops.", "content": "An experiment was performed on 31 guinea pigs to study the effect of fistulae of the horizontal canal, superior canal, common crus, and utricle on the course of experimentally induced endolymphatic hydrops. The effect of fistulae on corresponding parts of normal ears was also studied in an additional 17 animals. The results indicated a remarkable consistency of healing of the fistulae in both groups of animals. Fistulae had no significant effect on the course of endolymphatic hydrops, irrespective of whether the fistulae were made immediately before or several months after obliteration of the endolymphatic duct or whether a polyethylene strut was introduced into the membranous labyrinth. Once again, the functional significance of the endolymphatic sac was clearly apparent, for ablation of the sac consistently produced severe hydrops and atrophic changes in sensory and neural structures. Assuming that the mechanisms of labyrinth repair in the guinea pig are comparable to the higher mammalian ear, it is apparent that procedures designed to surgically fistulize the membranous labyrinth are of questionable value and are probably not rational therapeutic approaches to the management of M\u00e9ni\u00e8re's disease.", "contents": "Effect of fistulae on endolymphatic hydrops. An experiment was performed on 31 guinea pigs to study the effect of fistulae of the horizontal canal, superior canal, common crus, and utricle on the course of experimentally induced endolymphatic hydrops. The effect of fistulae on corresponding parts of normal ears was also studied in an additional 17 animals. The results indicated a remarkable consistency of healing of the fistulae in both groups of animals. Fistulae had no significant effect on the course of endolymphatic hydrops, irrespective of whether the fistulae were made immediately before or several months after obliteration of the endolymphatic duct or whether a polyethylene strut was introduced into the membranous labyrinth. Once again, the functional significance of the endolymphatic sac was clearly apparent, for ablation of the sac consistently produced severe hydrops and atrophic changes in sensory and neural structures. Assuming that the mechanisms of labyrinth repair in the guinea pig are comparable to the higher mammalian ear, it is apparent that procedures designed to surgically fistulize the membranous labyrinth are of questionable value and are probably not rational therapeutic approaches to the management of M\u00e9ni\u00e8re's disease."}
{"id": "PMID:1079440", "title": "Osteogenesis imperfecta congenita and tarda: a temporal bone report.", "content": "The temporal bone report of an operated case of osteogenesis imperfecta tarda is presented. Histological examination confirmed the presence of bilateral fixation of the footplate by otosclerosis as the cause of the conductive hearing loss. Fragility of bony septae in the mastoid and of the stapedial crura were observed. Sensorineural impairment in later years with a reduction in neural elements in the cochlea appear related to the extent and activity of the otosclerotic foci. Additional temporal bone reports of three cases of osteogenesis imperfecta congenita show lack of deposition of the skein-like bone in the endochondral layer, sparse bony septae in marrow spaces and deficiency of the perosteal layer. The stapedial crura were thin and in two cases both were deformed and fractured.", "contents": "Osteogenesis imperfecta congenita and tarda: a temporal bone report. The temporal bone report of an operated case of osteogenesis imperfecta tarda is presented. Histological examination confirmed the presence of bilateral fixation of the footplate by otosclerosis as the cause of the conductive hearing loss. Fragility of bony septae in the mastoid and of the stapedial crura were observed. Sensorineural impairment in later years with a reduction in neural elements in the cochlea appear related to the extent and activity of the otosclerotic foci. Additional temporal bone reports of three cases of osteogenesis imperfecta congenita show lack of deposition of the skein-like bone in the endochondral layer, sparse bony septae in marrow spaces and deficiency of the perosteal layer. The stapedial crura were thin and in two cases both were deformed and fractured."}
{"id": "PMID:1079444", "title": "Diagnosis of an enteric duplication with pertechnetate 99mTc scanning.", "content": "A case of enteric duplication is reported in which preoperative pertechnetate (99m)Tc scanning demonstrated localized uptake in the region of the anatomic abnormality. This test is recommended for use in the evaluation of lower gastrointestinal bleeding in infants and children.", "contents": "Diagnosis of an enteric duplication with pertechnetate 99mTc scanning. A case of enteric duplication is reported in which preoperative pertechnetate (99m)Tc scanning demonstrated localized uptake in the region of the anatomic abnormality. This test is recommended for use in the evaluation of lower gastrointestinal bleeding in infants and children."}
{"id": "PMID:1079442", "title": "Electronystagmographic findings in central nervous system disease.", "content": "A helpful central sign on electronystagmography with eyes closed is hyper-excitability on a caloric test. Another sign is consistent nystagmus in the presence of normal calorics. With eyes open, calibration overshoot, gaze nystagmus, pendulum tracking, failure of tracking a pendulum were by far the most common central abnormality in the experience of the author.", "contents": "Electronystagmographic findings in central nervous system disease. A helpful central sign on electronystagmography with eyes closed is hyper-excitability on a caloric test. Another sign is consistent nystagmus in the presence of normal calorics. With eyes open, calibration overshoot, gaze nystagmus, pendulum tracking, failure of tracking a pendulum were by far the most common central abnormality in the experience of the author."}
{"id": "PMID:1079445", "title": "Localization of tissue plasminogen activator in relation to morphologic changes in human saphenous veins used as coronary artery bypass autografts.", "content": "Employing the histochemical fibrin slide technique, we studied the localization of fibrinolytically active sites in relation to morphologic changes in saphenous veins used as coronary artery bypass autografts. Of veins from 100 patients undergoing surgery for coronary heart disease, 63 samples revealed well-demarcated intimal fibrinolytic activity. Distinct foci of lysis were present in the media and adventitia of all vein samples corresponding to the distribution of the vasa vasorum. Graft specimens obtained by autopsy from 13 patients surviving from 30 minutes to 13 days revealed considerable intimal damage with loss of fibrinolytic activity. Medial fibrinolytic activity disappeared early concomitant with disorganization of the medial muscle fibers, followed by necrosis, atrophy and fibrous transformation of the muscular structures. Adventitial activity disappeared later. One reoperation sample obtained after 8 weeks in situ showed moderate proliferative intimal fibrosis without an apparent neo-intima and without fibrinolytic activity, and one section showed evidence of valve cusp fibrosis, while most of the media had been replaced by fibrous tissue. Numerous foci of \"medial\" -adventitial fibrinolytic activity indicated presence of vascular structures in the venous wall. The fibrin slide technique is a convenient tool for studying the process of revascularization.", "contents": "Localization of tissue plasminogen activator in relation to morphologic changes in human saphenous veins used as coronary artery bypass autografts. Employing the histochemical fibrin slide technique, we studied the localization of fibrinolytically active sites in relation to morphologic changes in saphenous veins used as coronary artery bypass autografts. Of veins from 100 patients undergoing surgery for coronary heart disease, 63 samples revealed well-demarcated intimal fibrinolytic activity. Distinct foci of lysis were present in the media and adventitia of all vein samples corresponding to the distribution of the vasa vasorum. Graft specimens obtained by autopsy from 13 patients surviving from 30 minutes to 13 days revealed considerable intimal damage with loss of fibrinolytic activity. Medial fibrinolytic activity disappeared early concomitant with disorganization of the medial muscle fibers, followed by necrosis, atrophy and fibrous transformation of the muscular structures. Adventitial activity disappeared later. One reoperation sample obtained after 8 weeks in situ showed moderate proliferative intimal fibrosis without an apparent neo-intima and without fibrinolytic activity, and one section showed evidence of valve cusp fibrosis, while most of the media had been replaced by fibrous tissue. Numerous foci of \"medial\" -adventitial fibrinolytic activity indicated presence of vascular structures in the venous wall. The fibrin slide technique is a convenient tool for studying the process of revascularization."}
{"id": "PMID:1079446", "title": "Current management of upper gastrointestinal bleeding.", "content": "Over a four-year period, 585 patients were hospitalized for massive upper gastrointestinal bleeding. Endoscopy diagnosed the cause of bleeding in 80% of 200 patients so studied. Selective angiography localized the bleeding site in 12 of 20 patients, and infusion of vasopressor stopped hemorrhage in six. Barium studies was 90% accurate in diagnosing ulcer disease but failed to detect gastritis. One hundred thirty (22%) patients were operated upon for medically uncontrolled bleeding. The proportion of patients requiring surgery fell from 33% in year one to 13% in year four. Benign ulcer disease caused bleeding in 51% of surgical patients, while gastritis was found in 20%, esophageal varices in 15% and stress ulcer in 8%. Overall operative mortality was 29%. Among 38 duodenal ulcer patients, mortality was 18%. Vagotomy and pyloroplasty were more effective than resection in this group. Resection for distal gastric ulcers in 22 patients resulted in a mortality of 14% and no rebleeding. While V&P controlled bleeding in 12 alcoholics with gastritis, five (42%) died postoperatively. Mortality among 20 patients with esophageal varices was 35%, although all five survived who had porto-caval shunts. Eight of 10 patients operated upon for stress ulcer bleeding died. Postoperative rebleeding occurred in 14 patients, eight of whom were again operated upon. In all but one a new lesion was found to be responsible for hemorrhage. Increasing use of gastroscopy and selective angiography can be expected to improve diagnostic capabilities in patients with upper gastrointestinal bleeding. Infusing vasopressor into selected arteries should reduce the need for surgical control of gastritis, variceal and stress ulcer bleeding, conditions poorly managed by current operative techniques.", "contents": "Current management of upper gastrointestinal bleeding. Over a four-year period, 585 patients were hospitalized for massive upper gastrointestinal bleeding. Endoscopy diagnosed the cause of bleeding in 80% of 200 patients so studied. Selective angiography localized the bleeding site in 12 of 20 patients, and infusion of vasopressor stopped hemorrhage in six. Barium studies was 90% accurate in diagnosing ulcer disease but failed to detect gastritis. One hundred thirty (22%) patients were operated upon for medically uncontrolled bleeding. The proportion of patients requiring surgery fell from 33% in year one to 13% in year four. Benign ulcer disease caused bleeding in 51% of surgical patients, while gastritis was found in 20%, esophageal varices in 15% and stress ulcer in 8%. Overall operative mortality was 29%. Among 38 duodenal ulcer patients, mortality was 18%. Vagotomy and pyloroplasty were more effective than resection in this group. Resection for distal gastric ulcers in 22 patients resulted in a mortality of 14% and no rebleeding. While V&P controlled bleeding in 12 alcoholics with gastritis, five (42%) died postoperatively. Mortality among 20 patients with esophageal varices was 35%, although all five survived who had porto-caval shunts. Eight of 10 patients operated upon for stress ulcer bleeding died. Postoperative rebleeding occurred in 14 patients, eight of whom were again operated upon. In all but one a new lesion was found to be responsible for hemorrhage. Increasing use of gastroscopy and selective angiography can be expected to improve diagnostic capabilities in patients with upper gastrointestinal bleeding. Infusing vasopressor into selected arteries should reduce the need for surgical control of gastritis, variceal and stress ulcer bleeding, conditions poorly managed by current operative techniques."}
{"id": "PMID:1079447", "title": "Giant horseshoe intra-abdominal abscess.", "content": "A study of 12 patients with giant horseshoe abscess of the abdominal and pelvic cavities seen at the Surgical Services of the University of Cincinnati Medical Center has emphasized the complexity and bizarre nature of these lesions. These infections represented a huge abscess or series of communicating abscesses extending from one subphrenic space along the corresponding paracolic gutter into the pelvis, up and along the opposite paracolic space, and into the other subphrenic space. Since these lesions occurred infrequently, they were often not recognized until they had become far advanced and had produced profound effects on the patients. The diagnosis was difficult and obscured by various factors including the postoperative state after laparotomy for complex diseases or serious injuries of the biliary tract, the genitourinary tract, or the alimentary tract. An important etiologic component of the formation of these giant abscesses was the continuing escape and collection of large volumes of fluid resulting from lesions of the biliary tract, postoperative hemorrhage, or an unrecognized large perforated peptic ulcer. Nine patients were treated successfully and 3 died. The many diagnostic and therapeutic problems presented by the patients with this interesting and complex lesion have emphasized the importance of earlier and more accurate diagnosis, early and adequate surgical drainage, intelligently applied antibiotic therapy and appropriate supportive treatment. Failure to recognize and drain effectively each of the component sections of this lesion led to continuing sepsis with prolonged morbidity, progressive debility, and death.", "contents": "Giant horseshoe intra-abdominal abscess. A study of 12 patients with giant horseshoe abscess of the abdominal and pelvic cavities seen at the Surgical Services of the University of Cincinnati Medical Center has emphasized the complexity and bizarre nature of these lesions. These infections represented a huge abscess or series of communicating abscesses extending from one subphrenic space along the corresponding paracolic gutter into the pelvis, up and along the opposite paracolic space, and into the other subphrenic space. Since these lesions occurred infrequently, they were often not recognized until they had become far advanced and had produced profound effects on the patients. The diagnosis was difficult and obscured by various factors including the postoperative state after laparotomy for complex diseases or serious injuries of the biliary tract, the genitourinary tract, or the alimentary tract. An important etiologic component of the formation of these giant abscesses was the continuing escape and collection of large volumes of fluid resulting from lesions of the biliary tract, postoperative hemorrhage, or an unrecognized large perforated peptic ulcer. Nine patients were treated successfully and 3 died. The many diagnostic and therapeutic problems presented by the patients with this interesting and complex lesion have emphasized the importance of earlier and more accurate diagnosis, early and adequate surgical drainage, intelligently applied antibiotic therapy and appropriate supportive treatment. Failure to recognize and drain effectively each of the component sections of this lesion led to continuing sepsis with prolonged morbidity, progressive debility, and death."}
{"id": "PMID:1079448", "title": "Membrane vs bubble oxygenator: clinical comparison.", "content": "Numerous studies have demonstrated the superiority of membrane oxygenators (MO) over the bubble oxygenators (BO) when used for prolonged cardiopulmonary support. However, there is little information available evaluating the MO for routine, short-term cardiopulmonary bypass. In this study the 5MO314 Modulung-Teflo (MO) was compared to 5M30314 Miniprime Variflo (BO). The data of 91 patients (46 MO and 45 BO) were analyzed according to the duration of cardiopulmonary bypass (Group I less than 60 min., Group II 60-90 min. and Group III greater than 90 min.). Hemodynamic parameters, fluid and blood balance, as well as hematologic and blood gas studies were used for comparing the two oxygentors. The hemodynamic parameters were better, and the arterial blood gases were more physilogic with the MO. The postoperative blood loss was significantly less when using the MO. The other measurements documented the stability of the MO. All statements were based on statistical analysis with a DEC PDP-9 computer, using the MIIS language and operating system. Consequently, we are now using this MO for routine cardiopulmonary bypass.", "contents": "Membrane vs bubble oxygenator: clinical comparison. Numerous studies have demonstrated the superiority of membrane oxygenators (MO) over the bubble oxygenators (BO) when used for prolonged cardiopulmonary support. However, there is little information available evaluating the MO for routine, short-term cardiopulmonary bypass. In this study the 5MO314 Modulung-Teflo (MO) was compared to 5M30314 Miniprime Variflo (BO). The data of 91 patients (46 MO and 45 BO) were analyzed according to the duration of cardiopulmonary bypass (Group I less than 60 min., Group II 60-90 min. and Group III greater than 90 min.). Hemodynamic parameters, fluid and blood balance, as well as hematologic and blood gas studies were used for comparing the two oxygentors. The hemodynamic parameters were better, and the arterial blood gases were more physilogic with the MO. The postoperative blood loss was significantly less when using the MO. The other measurements documented the stability of the MO. All statements were based on statistical analysis with a DEC PDP-9 computer, using the MIIS language and operating system. Consequently, we are now using this MO for routine cardiopulmonary bypass."}
{"id": "PMID:1079449", "title": "The surgical treatment of unstable angina pectoris.", "content": "Since the advent of saphenous vein bypass grafting as successful means of myocardial revascularization, a variety of coronary artery disease syndrome have come under surgical attack. The proper role of surgery in many of these coronary syndromes remains ill-defined. However, clear indications for surgical revascularization exist in patients with unstable angina pectoris, i.e., progressive angina and onset of rest pain and noctural angina in spite of adequate medical therapy. An analysis has been made of 100 consecutive patients with unstable angina pectoris who underwent myocardial revascularization over the past 2 years at the Woodruff Medical Center of Emory University. Included in this group are the following subgroups: 1) Emergency cases with pre-infarction angina (including Printzmetal angina); 2) Cases of combined valvular heart disease and coronary artery disease; and 3) Advanced coronary artery disease with certain complications of previous myocardial infarction. A discussion of the relative merits of saphenous vein grafts and internal mammary artery anastomoses is presented and indicates that the technique selected should be determined by the quality of the distal native coronary circulation. Surgical mortality and morbidity figures, patency rates of saphenous vein grafts and internal mammary artery anastomoses visualized postoperatively, and the number of patients wiht dramatic relief of angina pectoris in this series support current enthusiasms for available surgical techniques for myocardial revascularization.", "contents": "The surgical treatment of unstable angina pectoris. Since the advent of saphenous vein bypass grafting as successful means of myocardial revascularization, a variety of coronary artery disease syndrome have come under surgical attack. The proper role of surgery in many of these coronary syndromes remains ill-defined. However, clear indications for surgical revascularization exist in patients with unstable angina pectoris, i.e., progressive angina and onset of rest pain and noctural angina in spite of adequate medical therapy. An analysis has been made of 100 consecutive patients with unstable angina pectoris who underwent myocardial revascularization over the past 2 years at the Woodruff Medical Center of Emory University. Included in this group are the following subgroups: 1) Emergency cases with pre-infarction angina (including Printzmetal angina); 2) Cases of combined valvular heart disease and coronary artery disease; and 3) Advanced coronary artery disease with certain complications of previous myocardial infarction. A discussion of the relative merits of saphenous vein grafts and internal mammary artery anastomoses is presented and indicates that the technique selected should be determined by the quality of the distal native coronary circulation. Surgical mortality and morbidity figures, patency rates of saphenous vein grafts and internal mammary artery anastomoses visualized postoperatively, and the number of patients wiht dramatic relief of angina pectoris in this series support current enthusiasms for available surgical techniques for myocardial revascularization."}
{"id": "PMID:1079450", "title": "Conventional splenorenal shunts. A reconsideration.", "content": "From 1961 to 1971, 73 central splenorenal and 66 portacaval shunts were done for cirrhotic patients who had bled from esophageal varices. Comparative analysis revealed low (3% and 2%, respectively) operative mortality for elective operations, equal (93%) effectiveness in control of variceal bleeding, but substantial diferences in the incidence of postshunt encephalopathy. For patients who had mimal hepatic dysfunction before operation, disabling disorders in mentation developed in 5% of patients who had splenorenal shunts, in contrast to 50% of patients who had portacaval shunts. Survival rates after the two shunts were nearly identical. Thus, the advantages of splenorenal shunts concern the quality of life but not the length of survival. These observations are considered in relation to available therapeutic alternatives.", "contents": "Conventional splenorenal shunts. A reconsideration. From 1961 to 1971, 73 central splenorenal and 66 portacaval shunts were done for cirrhotic patients who had bled from esophageal varices. Comparative analysis revealed low (3% and 2%, respectively) operative mortality for elective operations, equal (93%) effectiveness in control of variceal bleeding, but substantial diferences in the incidence of postshunt encephalopathy. For patients who had mimal hepatic dysfunction before operation, disabling disorders in mentation developed in 5% of patients who had splenorenal shunts, in contrast to 50% of patients who had portacaval shunts. Survival rates after the two shunts were nearly identical. Thus, the advantages of splenorenal shunts concern the quality of life but not the length of survival. These observations are considered in relation to available therapeutic alternatives."}
{"id": "PMID:1079451", "title": "Occult gastrointestinal bleeding. An evaluation of available diagnostic methods.", "content": "Occult gastrointestinal bleeding was defined as continued bleeding in spite of a normal series of roentgenorgrams of the upper part of the gastrointestinal tract, barium enema, and sigmoidoscopy. Twenty-six such patients were treated. A thorough systematic evaluation, including gastroscopy, colonoscopy, visceral angiography, and isotopic scanning, was done preoperatively. Using colonoscopy and arteriography, nearly 60% of the bleeding sites were identified. Seventy-six percent of the lesions identified were in the terminal part of the ileum or the ascending colon. Exploratory laparotomy should be performed for life-threatening hemorrhage or as a diagnostic test only after a thorough preoperative evaluation. If results of a complete preoperative evaluation including arteriography were normal, then the likelihood of finding a discrete cause of bleeding at laparotomy was high (80%). A systematic evaluation and diligence of both physcian and patient in localizing the site of bleeding are essential.", "contents": "Occult gastrointestinal bleeding. An evaluation of available diagnostic methods. Occult gastrointestinal bleeding was defined as continued bleeding in spite of a normal series of roentgenorgrams of the upper part of the gastrointestinal tract, barium enema, and sigmoidoscopy. Twenty-six such patients were treated. A thorough systematic evaluation, including gastroscopy, colonoscopy, visceral angiography, and isotopic scanning, was done preoperatively. Using colonoscopy and arteriography, nearly 60% of the bleeding sites were identified. Seventy-six percent of the lesions identified were in the terminal part of the ileum or the ascending colon. Exploratory laparotomy should be performed for life-threatening hemorrhage or as a diagnostic test only after a thorough preoperative evaluation. If results of a complete preoperative evaluation including arteriography were normal, then the likelihood of finding a discrete cause of bleeding at laparotomy was high (80%). A systematic evaluation and diligence of both physcian and patient in localizing the site of bleeding are essential."}
{"id": "PMID:1079453", "title": "Adenotonsillectomy in a Jehovah's Witness with blood dyscrasia.", "content": "A case is used to illustrate a method of management of three unusual problems all simultaneously appearing in the same patient. Urgent surgical intervention was necessary for a child with a bleeding disorder, but the parents religious doctrine prohibited the use of whole blood or its products. The combined efforts of the legal, medical, and allied medical professionals led to a successful resolution of the patient's multiple problems.", "contents": "Adenotonsillectomy in a Jehovah's Witness with blood dyscrasia. A case is used to illustrate a method of management of three unusual problems all simultaneously appearing in the same patient. Urgent surgical intervention was necessary for a child with a bleeding disorder, but the parents religious doctrine prohibited the use of whole blood or its products. The combined efforts of the legal, medical, and allied medical professionals led to a successful resolution of the patient's multiple problems."}
{"id": "PMID:1079454", "title": "[The two-phase occurrence of head-shaking nystagmus (author's transl)].", "content": "The report deals with 22 cases of two-phase head-shaking nystagmus. The results can be summarised as follows: 1. The two-phase head-shaking nystagmus is only found in connection with peripheral-vestibular disorders and particularly with unilateral disorders. It occurs only temporarily in the course of the vestibule disorder and may be observable for several months. 2. The head-shaking nystagmus, which follows immediately after the head-shaking (first phase), is to be considered a deficiency nystagmus. After a short period of latency after the disappearance of the first phase, a nystagmus in the opposite direction appears (second phase). This is considered to be a recovery nystagmus. 3. In the case of unilateral labyrinth lesions, the direction of nystagmus of the second phase of two-phase head-shaking nystagmus indicates the side of the lesion. The functions of the diseased labyrinth, however, have not been completely destroyed in this case. In the case of bilateral lesions, the direction of nystagmus of the second phase probably points toward the more heavily damaged side. While it is found only rarely, the two-phase head-shaking nystagmus thus permits reliable diagnosis and detection of the probable side where a peripheral vestibule disorder is located.", "contents": "[The two-phase occurrence of head-shaking nystagmus (author's transl)]. The report deals with 22 cases of two-phase head-shaking nystagmus. The results can be summarised as follows: 1. The two-phase head-shaking nystagmus is only found in connection with peripheral-vestibular disorders and particularly with unilateral disorders. It occurs only temporarily in the course of the vestibule disorder and may be observable for several months. 2. The head-shaking nystagmus, which follows immediately after the head-shaking (first phase), is to be considered a deficiency nystagmus. After a short period of latency after the disappearance of the first phase, a nystagmus in the opposite direction appears (second phase). This is considered to be a recovery nystagmus. 3. In the case of unilateral labyrinth lesions, the direction of nystagmus of the second phase of two-phase head-shaking nystagmus indicates the side of the lesion. The functions of the diseased labyrinth, however, have not been completely destroyed in this case. In the case of bilateral lesions, the direction of nystagmus of the second phase probably points toward the more heavily damaged side. While it is found only rarely, the two-phase head-shaking nystagmus thus permits reliable diagnosis and detection of the probable side where a peripheral vestibule disorder is located."}
{"id": "PMID:1079455", "title": "Predictive validities of several clinical color vision tests for aviation signal light gun performance.", "content": "Scores on the American Optical Company (AOC) test (1965 edition), Dvorine test, Farnsworth Lantern test, Color Threshold Tester, Farnsworth-Munsell 100-Hue test, Farnsworth Panel D-15 test, and Schmidt-Haensch Anomaloscope were obtained from 137 men with color-defective vision and 128 men with normal color vision. The validity of each of these tests in predicting scores on the aviation signal light gun was assessed by using daytime and nighttime administrations of the light gun as the criteria. Two \"best sets\" of plates from the AOC and Dvorine tests were selected by calculating a multiple regression equation in a stepwise manner with the nighttime and then the daytime administration of the signal light gun test as the criteria. Based on a graphic presentation of the miss and false alarm rates for each test at various possible cut scores, suggestions were made regarding the use of each test and the selection of optimal pass/fail scores.", "contents": "Predictive validities of several clinical color vision tests for aviation signal light gun performance. Scores on the American Optical Company (AOC) test (1965 edition), Dvorine test, Farnsworth Lantern test, Color Threshold Tester, Farnsworth-Munsell 100-Hue test, Farnsworth Panel D-15 test, and Schmidt-Haensch Anomaloscope were obtained from 137 men with color-defective vision and 128 men with normal color vision. The validity of each of these tests in predicting scores on the aviation signal light gun was assessed by using daytime and nighttime administrations of the light gun as the criteria. Two \"best sets\" of plates from the AOC and Dvorine tests were selected by calculating a multiple regression equation in a stepwise manner with the nighttime and then the daytime administration of the signal light gun test as the criteria. Based on a graphic presentation of the miss and false alarm rates for each test at various possible cut scores, suggestions were made regarding the use of each test and the selection of optimal pass/fail scores."}
{"id": "PMID:1079458", "title": "A comparison of the effects of ouabain and 2-deoxy-D-glucose on the thermodynamic variables of the frog skin.", "content": "Previous studies support the validity of a linear thermodynamic formalism relating the rates of active Na-+ transport and oxygen consumption Jr to the electrical potential difference delta-psi and the affinity A (negative free energy) of the metabolic driving reaction. The formulation was further tested in paired control and experimental hemiskins by the use of two inhibitors of Na-+ transport. Ouabain, a specific inhibitor of the Na-+ pump, might be expected to diminish the dependence of Jr on delta-psi without affecting A, whereas 2-deoxy-D-glucose, a competitive inhibitor of glucose metabolism should be expected to diminish A. Both inhibitors were used at concentrations adequate to depress Na-+ transport (i.e. short-circuit current Io) to some 50% of control level. Measurements were made of Io and dJr/d(delat-psi), and the apparent value of the affinity Aapp was calculated according to the thermodynamic formulation. Ouabain depressed minus dJr/d(delta-psi) without affecting Aapp whereas 2-deoxy-D-glucose depressed Aapp without affecting minus dJr/d(delta-psi). The demonstration of these effects indicated the utility of the formalism.", "contents": "A comparison of the effects of ouabain and 2-deoxy-D-glucose on the thermodynamic variables of the frog skin. Previous studies support the validity of a linear thermodynamic formalism relating the rates of active Na-+ transport and oxygen consumption Jr to the electrical potential difference delta-psi and the affinity A (negative free energy) of the metabolic driving reaction. The formulation was further tested in paired control and experimental hemiskins by the use of two inhibitors of Na-+ transport. Ouabain, a specific inhibitor of the Na-+ pump, might be expected to diminish the dependence of Jr on delta-psi without affecting A, whereas 2-deoxy-D-glucose, a competitive inhibitor of glucose metabolism should be expected to diminish A. Both inhibitors were used at concentrations adequate to depress Na-+ transport (i.e. short-circuit current Io) to some 50% of control level. Measurements were made of Io and dJr/d(delat-psi), and the apparent value of the affinity Aapp was calculated according to the thermodynamic formulation. Ouabain depressed minus dJr/d(delta-psi) without affecting Aapp whereas 2-deoxy-D-glucose depressed Aapp without affecting minus dJr/d(delta-psi). The demonstration of these effects indicated the utility of the formalism."}
{"id": "PMID:1079460", "title": "[Lymphocyte in chronic lymphatic leukemia].", "content": "In patients with chronic lymphatic leukemia (CLL) the majority of peripheral blood lymphocytes was characterized as B cells by surface membrane markers. Ultrastructural studies revealed a reduction of the cytoplasmic area. Furthermore, the number of lysosomes was diminished corresponding to a decreased activity of lysosomal hydrolases. Increasing blood lymphocytosis was paralleled by an increase of the percentage of lysosome-poor lymphocytes. Response of CLL lymphocytes to in vitro stimulation with PHA and PWM was either lacking or diminished and/or delayed, and the number of transformed cells was reduced. Thus, the majority of CLL lymphocytes appears to represent both morphologically and functionally abnormal neoplastic b cells. During the early and later phase of stimulation the mitogen-reactive CLL lymphocytes exhibited alterations of the lysosomal apparatus similar to those observed in normal cells. The reactive lymphocytes may be derived from residual populations of normally functioning T and/or B cells. However, the neoplastic cells may also be able to respond to the mitogens. In vivo studies showed impaired kinetics of circulation and recirculation of CLL B lymphocytes, whereas the T cells were normal in this respect.", "contents": "[Lymphocyte in chronic lymphatic leukemia]. In patients with chronic lymphatic leukemia (CLL) the majority of peripheral blood lymphocytes was characterized as B cells by surface membrane markers. Ultrastructural studies revealed a reduction of the cytoplasmic area. Furthermore, the number of lysosomes was diminished corresponding to a decreased activity of lysosomal hydrolases. Increasing blood lymphocytosis was paralleled by an increase of the percentage of lysosome-poor lymphocytes. Response of CLL lymphocytes to in vitro stimulation with PHA and PWM was either lacking or diminished and/or delayed, and the number of transformed cells was reduced. Thus, the majority of CLL lymphocytes appears to represent both morphologically and functionally abnormal neoplastic b cells. During the early and later phase of stimulation the mitogen-reactive CLL lymphocytes exhibited alterations of the lysosomal apparatus similar to those observed in normal cells. The reactive lymphocytes may be derived from residual populations of normally functioning T and/or B cells. However, the neoplastic cells may also be able to respond to the mitogens. In vivo studies showed impaired kinetics of circulation and recirculation of CLL B lymphocytes, whereas the T cells were normal in this respect."}
{"id": "PMID:1079461", "title": "A study of 137 cases of renal trauma.", "content": "Renal injuries are rarely a threat to life and all patients should have an intravenous urogram performed at the time of admission. Patients with a normal urogram may be mobilised for early discharge. Patients with minor abnormalities of the urogram, but in whom all the collecting system is visible, rarely caused any difficulties in management. Should the haematuria persist beyond a week, then arteriography should be performed. The arteriogram sometimes shows the presence of major cortical lacerations with minor abnormalities of the intravenous urogram. If the initial urogram fails to opacify the injured kidney, immediate arteriography is required to exclude a vascular injury. We also believe that early arteriography is advisable in those patients where part of the kidney appears to be non-functioning as it is in this group of patients that operation may become necessary. The indications for renal arteriography are summarised in Table II.", "contents": "A study of 137 cases of renal trauma. Renal injuries are rarely a threat to life and all patients should have an intravenous urogram performed at the time of admission. Patients with a normal urogram may be mobilised for early discharge. Patients with minor abnormalities of the urogram, but in whom all the collecting system is visible, rarely caused any difficulties in management. Should the haematuria persist beyond a week, then arteriography should be performed. The arteriogram sometimes shows the presence of major cortical lacerations with minor abnormalities of the intravenous urogram. If the initial urogram fails to opacify the injured kidney, immediate arteriography is required to exclude a vascular injury. We also believe that early arteriography is advisable in those patients where part of the kidney appears to be non-functioning as it is in this group of patients that operation may become necessary. The indications for renal arteriography are summarised in Table II."}
{"id": "PMID:1079468", "title": "Responses of spinal neurons to iontophoretically applied prostaglandin E1 in the frog.", "content": "Prostaglandin E1 (PGE1) has been tested by microiontophoresis on interneurons and motoneurons in the isolated spinal cord of the frog. PGE1 action was almost exclusively excitatory, regardless of the cell type and the test condition used. In general, the excitatory response had rapid onset and termination, and was susceptible to tachyphylaxis. Responsive interneurons were scattered throughout the tissue, and the incidence of responses showed no obvious relation to the mono- vs. polysynaptic connection of cells to primary afferents and descending fibers of the lateral funiculus. PGE1-sensitive interneurons were encountered more often in preparations from summer than from winter animals. These findings, coupled with the demonstration of prostaglandin biosynthesis and catabolism in the tissue, suggest that PGE1 plays a role in the normal function of spinal neurons. The intimate mechanism of PGE1 action remains to be elucidated.", "contents": "Responses of spinal neurons to iontophoretically applied prostaglandin E1 in the frog. Prostaglandin E1 (PGE1) has been tested by microiontophoresis on interneurons and motoneurons in the isolated spinal cord of the frog. PGE1 action was almost exclusively excitatory, regardless of the cell type and the test condition used. In general, the excitatory response had rapid onset and termination, and was susceptible to tachyphylaxis. Responsive interneurons were scattered throughout the tissue, and the incidence of responses showed no obvious relation to the mono- vs. polysynaptic connection of cells to primary afferents and descending fibers of the lateral funiculus. PGE1-sensitive interneurons were encountered more often in preparations from summer than from winter animals. These findings, coupled with the demonstration of prostaglandin biosynthesis and catabolism in the tissue, suggest that PGE1 plays a role in the normal function of spinal neurons. The intimate mechanism of PGE1 action remains to be elucidated."}
{"id": "PMID:1079469", "title": "Treatment of Pneumocystis carinii pneumonitis with trimethoprim-sulfamethoxazole.", "content": "Pneumocystis carinii pneumonitis (PCP) is fatal in 90 to 100% of the cases if no treatment is given. Trimethoprim-sulfamethoxazole (TMP-SMX) was used at one of two dosage levels in the treatment of 20 children with PCP and cancer. Of 14 patients treated with 20 mg TMP--100 mg SMX/kgd, 12 recovered and 2 died. Treatment of the fatal cases and one of the patients who recovered was supplemented with pentamidine. When six patients were treated with 4 to 7 mg TMP--20 to 35 mg SMX/kgd, four recovered and two died. Both fatal cases and one of the patients who recovered were also treated with pentamidine. There was no significant adverse effects from TMP-SMX.", "contents": "Treatment of Pneumocystis carinii pneumonitis with trimethoprim-sulfamethoxazole. Pneumocystis carinii pneumonitis (PCP) is fatal in 90 to 100% of the cases if no treatment is given. Trimethoprim-sulfamethoxazole (TMP-SMX) was used at one of two dosage levels in the treatment of 20 children with PCP and cancer. Of 14 patients treated with 20 mg TMP--100 mg SMX/kgd, 12 recovered and 2 died. Treatment of the fatal cases and one of the patients who recovered was supplemented with pentamidine. When six patients were treated with 4 to 7 mg TMP--20 to 35 mg SMX/kgd, four recovered and two died. Both fatal cases and one of the patients who recovered were also treated with pentamidine. There was no significant adverse effects from TMP-SMX."}
{"id": "PMID:1079471", "title": "Immunologic parameters of children with urinary tract infection: effects of trimethoprim-sulfamethoxazole.", "content": "In order to determine whether a deficiency in immunologic response predisposes certain children to recurrent infections of the urinary tract, four groups of children were investigated: a control group; children with extraurinary infections; children with urinary tract infections; and a group of children treated with trimethoprim-sulfamethoxazole (TMP-SMX). In none of the groups were there changes in humoral immunoglobulins, peripheral neutrophil counts, serum complement concentrations or urinary excretion of IgG, IgA, or IgM that might might predispose to infection. However, children with urinary tract infections were more likely to belong to blood group A (66.6%; expected frequency, 45%) and had a blunted thymidine uptake of their stimulated lymphocytes (RLB) when compared with children with extraurinary infection. As well, their nitroblue tetrazolium reduction (NBT) was significantly lowered and this paralleled their RLB response. We postulated a shared antigenic feature of either their renal-urinary tissue or bacterial antigen with blood group A antigen; this prevents the mounting of an effective immunologic defence. If TMP-SMX further depresses the lymphocyte response, it may be considered contraindicated in urinary tract infection. In 11 children treated with this drug we found no significant difference between their RLB and NBT responses and those of children with infections of the urinary system treated with other drugs. We conclude that TMP-SMX does not alter the immune responses in children.", "contents": "Immunologic parameters of children with urinary tract infection: effects of trimethoprim-sulfamethoxazole. In order to determine whether a deficiency in immunologic response predisposes certain children to recurrent infections of the urinary tract, four groups of children were investigated: a control group; children with extraurinary infections; children with urinary tract infections; and a group of children treated with trimethoprim-sulfamethoxazole (TMP-SMX). In none of the groups were there changes in humoral immunoglobulins, peripheral neutrophil counts, serum complement concentrations or urinary excretion of IgG, IgA, or IgM that might might predispose to infection. However, children with urinary tract infections were more likely to belong to blood group A (66.6%; expected frequency, 45%) and had a blunted thymidine uptake of their stimulated lymphocytes (RLB) when compared with children with extraurinary infection. As well, their nitroblue tetrazolium reduction (NBT) was significantly lowered and this paralleled their RLB response. We postulated a shared antigenic feature of either their renal-urinary tissue or bacterial antigen with blood group A antigen; this prevents the mounting of an effective immunologic defence. If TMP-SMX further depresses the lymphocyte response, it may be considered contraindicated in urinary tract infection. In 11 children treated with this drug we found no significant difference between their RLB and NBT responses and those of children with infections of the urinary system treated with other drugs. We conclude that TMP-SMX does not alter the immune responses in children."}
{"id": "PMID:1079472", "title": "Quantitation of T- and B-lymphocytes in peripheral blood of patients with solid tumours. I. Relation to other parameters of in vivo and in vitro immune competence.", "content": "The absolute number of T- and B-lymphocytes in the peripheral blood was measured in 22 patients with disseminated nonlymphoid solid malignant tumours. Patients with normal absolute lymphocyte counts had normal absolute numbers of T- and B-lymphocytes; patients with low counts had low numbers. The percentage of T- and B-lymphocytes was similar, in both groups of patients, to that of healthy control subjects. Patients with a normal absolute lymphocyte count had better in vitro lymphocyte responses to mitogenic agents than did patients with low absolute lymphocyte counts. Improvement of depressed in vitro lymphocyte responses to normal on culture of cells in AB plasma was most likely in patients with normal absolute lymphocyte counts. The absolute lymphocyte count by itself, then, provides useful information about the immune status of patients with solid malignant tumours.", "contents": "Quantitation of T- and B-lymphocytes in peripheral blood of patients with solid tumours. I. Relation to other parameters of in vivo and in vitro immune competence. The absolute number of T- and B-lymphocytes in the peripheral blood was measured in 22 patients with disseminated nonlymphoid solid malignant tumours. Patients with normal absolute lymphocyte counts had normal absolute numbers of T- and B-lymphocytes; patients with low counts had low numbers. The percentage of T- and B-lymphocytes was similar, in both groups of patients, to that of healthy control subjects. Patients with a normal absolute lymphocyte count had better in vitro lymphocyte responses to mitogenic agents than did patients with low absolute lymphocyte counts. Improvement of depressed in vitro lymphocyte responses to normal on culture of cells in AB plasma was most likely in patients with normal absolute lymphocyte counts. The absolute lymphocyte count by itself, then, provides useful information about the immune status of patients with solid malignant tumours."}
{"id": "PMID:1079473", "title": "A rapid and specific radioimmunoassay for methotrexate.", "content": "A sensitive and precise radioimmunoassay for methotrexate has been developed using antibody induced in rabbits, tritium-labeled methotrexate, and a nitrocellulose membrane separation technique. Antibody specificity was characterized by comparing the effectiveness of various related compounds to displace labeled methotrexate from the antibody-binding site. Assay of serum samples from persons receiving the drug was rapid and easy to perform. In a pharmacokinetic study of methotrexate, corresponding results were obtained when measurements were made by either enzymic assay or by radioimmunoassay. Drug concentrations could also be monitored in the cerebrospinal fluid and urine of patients on high-dose methotrexate therapy followed by citrovorum factor rescue. The system measured a little as 0.1 to 1 pmole of methotrexate, depending upon the antiserum used, and naturally occurring folates did not interfere with these determinations.", "contents": "A rapid and specific radioimmunoassay for methotrexate. A sensitive and precise radioimmunoassay for methotrexate has been developed using antibody induced in rabbits, tritium-labeled methotrexate, and a nitrocellulose membrane separation technique. Antibody specificity was characterized by comparing the effectiveness of various related compounds to displace labeled methotrexate from the antibody-binding site. Assay of serum samples from persons receiving the drug was rapid and easy to perform. In a pharmacokinetic study of methotrexate, corresponding results were obtained when measurements were made by either enzymic assay or by radioimmunoassay. Drug concentrations could also be monitored in the cerebrospinal fluid and urine of patients on high-dose methotrexate therapy followed by citrovorum factor rescue. The system measured a little as 0.1 to 1 pmole of methotrexate, depending upon the antiserum used, and naturally occurring folates did not interfere with these determinations."}
{"id": "PMID:1079474", "title": "Adducts between the carcinogen 2-acetamidophenanthrene and adenine and guanine of DNA.", "content": "The sulfate and acetate esters of the carcinogen N-hydroxy-2-acetamidophenanthrene attack calf thymus DNA in vitro to yield adducts of 2-acetamidophenanthrene with guanine and adenine in the DNA. These adducts were found to be 8-(N-2-phenanthrylacetamido)deoxyguanosine and N6-1-(2-acetamidophenanthryl)deoxyadenosine, respectively. These reactions, and the already known reactions of esters of N-hydroxy-2-acetamidofluorene, together with H\u00fcckel molecular orbital calculations, suggest that the relative tendencies of a series of N-aryl-N-acetylnitrenium ions to react with adenine and guanine may be predicted.", "contents": "Adducts between the carcinogen 2-acetamidophenanthrene and adenine and guanine of DNA. The sulfate and acetate esters of the carcinogen N-hydroxy-2-acetamidophenanthrene attack calf thymus DNA in vitro to yield adducts of 2-acetamidophenanthrene with guanine and adenine in the DNA. These adducts were found to be 8-(N-2-phenanthrylacetamido)deoxyguanosine and N6-1-(2-acetamidophenanthryl)deoxyadenosine, respectively. These reactions, and the already known reactions of esters of N-hydroxy-2-acetamidofluorene, together with H\u00fcckel molecular orbital calculations, suggest that the relative tendencies of a series of N-aryl-N-acetylnitrenium ions to react with adenine and guanine may be predicted."}
{"id": "PMID:1079475", "title": "Two approaches that increase the activity of analogs of adenine nucleosides in animal cells.", "content": "Deamination of many analogs of adenine nucleosides results in the loss of their chemotherapeutic efficacy. Two approaches have been used in this study to overcome this problem. First, some adenine nucleotides, which are resistant to mammalian adenosine deaminase, are more toxic to animal cells than are the respective nucleosides. For toxic to animal cells than are the respective nucleosides. For example, 9-beta-D-arabinofuranosyladenine 5'-phosphate, a molecule that penetrates the cell without degradation, has a more sustained toxicity against mouse fibroblasts (L-cells) than does 9-beta-D-arabinofuranosyladenine (ara-A). Furthermore, L-cells treated with 2',3'-dideoxyadenosine 5'-phosphate are extensively killed after 48 hr, whereas 2',3'-dideoxyadenosine is almost nontoxic to L-cells. Specific inhibition of adenosine deaminase by nontoxic concentrations of erythro-9-(2-hydroxy-3-nonyl)adenine greatly potentiates the biological activity of both ara-A and 3'-deoxyadenosine (cordycepin). Simultaneous administration of cytostatic concentrations of ara-A and the inhibitor of adenosine deaminase to L-cells killed greater than 99.9 percent of cells in 36 hr. A similar concentration of ara-A plus the deaminase inhibitor also markedly extended the mean survival of mice bearing Ehrlich ascites carcinoma as compared to ara-A alone. A cytostatic concentration of cordycepin 1 x 10-4 M), administered in the presence of deaminase inhibitor, killed greater than 99.9 percent of cultured L-cells in only 8 hr. During the latter incubation, accumulation of uridine in acid-insoluble material reached a maximum after 30 min, and incorporation of thymidine into acid-insoluble material was almost totally arrested after 2 hr.", "contents": "Two approaches that increase the activity of analogs of adenine nucleosides in animal cells. Deamination of many analogs of adenine nucleosides results in the loss of their chemotherapeutic efficacy. Two approaches have been used in this study to overcome this problem. First, some adenine nucleotides, which are resistant to mammalian adenosine deaminase, are more toxic to animal cells than are the respective nucleosides. For toxic to animal cells than are the respective nucleosides. For example, 9-beta-D-arabinofuranosyladenine 5'-phosphate, a molecule that penetrates the cell without degradation, has a more sustained toxicity against mouse fibroblasts (L-cells) than does 9-beta-D-arabinofuranosyladenine (ara-A). Furthermore, L-cells treated with 2',3'-dideoxyadenosine 5'-phosphate are extensively killed after 48 hr, whereas 2',3'-dideoxyadenosine is almost nontoxic to L-cells. Specific inhibition of adenosine deaminase by nontoxic concentrations of erythro-9-(2-hydroxy-3-nonyl)adenine greatly potentiates the biological activity of both ara-A and 3'-deoxyadenosine (cordycepin). Simultaneous administration of cytostatic concentrations of ara-A and the inhibitor of adenosine deaminase to L-cells killed greater than 99.9 percent of cells in 36 hr. A similar concentration of ara-A plus the deaminase inhibitor also markedly extended the mean survival of mice bearing Ehrlich ascites carcinoma as compared to ara-A alone. A cytostatic concentration of cordycepin 1 x 10-4 M), administered in the presence of deaminase inhibitor, killed greater than 99.9 percent of cultured L-cells in only 8 hr. During the latter incubation, accumulation of uridine in acid-insoluble material reached a maximum after 30 min, and incorporation of thymidine into acid-insoluble material was almost totally arrested after 2 hr."}
{"id": "PMID:1079481", "title": "Anomalous left coronary artery from pulmonary artery. An eight year angiographic follow-up after saphenous vein bypass graft.", "content": "We have presented a 7-week and 8-year postoperative angiographic follow-up of a 36-year-old man who had an anomalous left coronary artery arising from the pulmonary artery and who had an aortocornary bypass graft to the left anterior descending artery. The late study showed 1) a large, well functioning patent graft, 2) lack of significant postoperative change in the size of the right coronary artery and 3) new collateral vessels between the distal left anterior descending and left circumflex coronary arteries and persistence of collateral vessels between the distal right and left circulflex coronary arteries. This report is the longest known angiographic follow-up of a patient with this anomaly who has been treated with bypass surgery.", "contents": "Anomalous left coronary artery from pulmonary artery. An eight year angiographic follow-up after saphenous vein bypass graft. We have presented a 7-week and 8-year postoperative angiographic follow-up of a 36-year-old man who had an anomalous left coronary artery arising from the pulmonary artery and who had an aortocornary bypass graft to the left anterior descending artery. The late study showed 1) a large, well functioning patent graft, 2) lack of significant postoperative change in the size of the right coronary artery and 3) new collateral vessels between the distal left anterior descending and left circumflex coronary arteries and persistence of collateral vessels between the distal right and left circulflex coronary arteries. This report is the longest known angiographic follow-up of a patient with this anomaly who has been treated with bypass surgery."}
{"id": "PMID:1079482", "title": "Ventricular function before and after direct revascularization surgery. A proposal for an index of vascularization to correlate angiographic and ventriculographic findings.", "content": "In order to determine the effect of direct bypass surgery on the total coronary arterial supply to the heart, a numerical construct was devised to incorporate the development of new obstructive lesions and the presence of patent or nonpatent grafts. This construct, termed a vascularization index (VI), was used to evaluate left ventricular function and anginal symptoms after coronary artery bypass surgery in 56 patients. Patients with an increase in local or total arterial blood supply (positive delts VI) often had similar directional changes in segmental wall motion and ventricular ejection fraction. Patients with a decrease in local or total arterial blood supply (negative delta VI), due to either nonpatent grafts or progression of atherosclerotic disease, also usually had similar directional changes in segmental wall motion and ventricular ejection fraction. (Although nearly all patients reported a decrease in anginal symptoms after surgery, delta VI could not identify degree of improvement.) Use of an integrated approach in describing pre and postoperative myocardial blood supply appears to be the most reliable method of explaining changes in left ventricular function.", "contents": "Ventricular function before and after direct revascularization surgery. A proposal for an index of vascularization to correlate angiographic and ventriculographic findings. In order to determine the effect of direct bypass surgery on the total coronary arterial supply to the heart, a numerical construct was devised to incorporate the development of new obstructive lesions and the presence of patent or nonpatent grafts. This construct, termed a vascularization index (VI), was used to evaluate left ventricular function and anginal symptoms after coronary artery bypass surgery in 56 patients. Patients with an increase in local or total arterial blood supply (positive delts VI) often had similar directional changes in segmental wall motion and ventricular ejection fraction. Patients with a decrease in local or total arterial blood supply (negative delta VI), due to either nonpatent grafts or progression of atherosclerotic disease, also usually had similar directional changes in segmental wall motion and ventricular ejection fraction. (Although nearly all patients reported a decrease in anginal symptoms after surgery, delta VI could not identify degree of improvement.) Use of an integrated approach in describing pre and postoperative myocardial blood supply appears to be the most reliable method of explaining changes in left ventricular function."}
{"id": "PMID:1079483", "title": "Myocardial revascularization combined with intracoronary infusion of hyperosmolar solution in the early management of postinfarction ventricular septal defect. Report of a case.", "content": "A patients is described with postinfarction ventricular septal defect in whom the perforation was successfully closed within 24 hours of septal rupture. This presents the second such case reported in the literature. Adjunctive measures consisting of myocardial revascularization and intracoronary infusion of mannitol were thought to be important in the successful outcome of the operative procedure. The importance of complete preoperative cardiac catheterization with coronary arteriography is stressed. The theoretical role of endothelial and myocardial cellular edema as a cause of depressed myocardial function immediately following an ischemic insult is proposed as a practical consideration in the high mortality associated with this condition. Methods used to prevent or reverse such cell swelling are described. The details of the operation in which viable ventricle myocardium was used to fill the septal defect are presented.", "contents": "Myocardial revascularization combined with intracoronary infusion of hyperosmolar solution in the early management of postinfarction ventricular septal defect. Report of a case. A patients is described with postinfarction ventricular septal defect in whom the perforation was successfully closed within 24 hours of septal rupture. This presents the second such case reported in the literature. Adjunctive measures consisting of myocardial revascularization and intracoronary infusion of mannitol were thought to be important in the successful outcome of the operative procedure. The importance of complete preoperative cardiac catheterization with coronary arteriography is stressed. The theoretical role of endothelial and myocardial cellular edema as a cause of depressed myocardial function immediately following an ischemic insult is proposed as a practical consideration in the high mortality associated with this condition. Methods used to prevent or reverse such cell swelling are described. The details of the operation in which viable ventricle myocardium was used to fill the septal defect are presented."}
{"id": "PMID:1079484", "title": "Hypotension accompanying the onset of exertional angina. A sign of severe compromise of left ventricular blood supply.", "content": "Serious obstructive coronary artery disease was found in all patients who developed hypotension accompanying the onset of angina during multistage exercise testing. Seventeen exercising patients demonstrated a fall in systolic pressure to below resting levels as chest pain and ST-segment depression appeared. Two patients died suddenly six weeks after treadmill testing and prior to arteriography. The remaining fifteen were studied with coronary arteriography and all except one exhibited greater than or equal to 90% stenosis of the left anterior descending artery (LAD). The remaining patient demonstrated two 75% LAD stenoses in series. Five exhibited significant (greater than or equal 75%) narrowing of the main left coronary artery (MLCA) and thirteen of fifteen had significant stenosis of proximal LAD and circumflex arteries. The two patients without significant circumflex disease exhibited greater than or equal to 90% stenosis of the dominant right coronaryartery (RCA) circulation. Six of six patients had restoration of a normal blood pressure response following coronary bypass surgery, which also relieved angina and reversed ST-segment depression. Conditions essential for proper interpretation of this sign are discussed. If these conditions are met, then a fall in systolic pressure during treadmill-induced angina pectoris is a reliable sign of severe compromise of left ventricular blood supply.", "contents": "Hypotension accompanying the onset of exertional angina. A sign of severe compromise of left ventricular blood supply. Serious obstructive coronary artery disease was found in all patients who developed hypotension accompanying the onset of angina during multistage exercise testing. Seventeen exercising patients demonstrated a fall in systolic pressure to below resting levels as chest pain and ST-segment depression appeared. Two patients died suddenly six weeks after treadmill testing and prior to arteriography. The remaining fifteen were studied with coronary arteriography and all except one exhibited greater than or equal to 90% stenosis of the left anterior descending artery (LAD). The remaining patient demonstrated two 75% LAD stenoses in series. Five exhibited significant (greater than or equal 75%) narrowing of the main left coronary artery (MLCA) and thirteen of fifteen had significant stenosis of proximal LAD and circumflex arteries. The two patients without significant circumflex disease exhibited greater than or equal to 90% stenosis of the dominant right coronaryartery (RCA) circulation. Six of six patients had restoration of a normal blood pressure response following coronary bypass surgery, which also relieved angina and reversed ST-segment depression. Conditions essential for proper interpretation of this sign are discussed. If these conditions are met, then a fall in systolic pressure during treadmill-induced angina pectoris is a reliable sign of severe compromise of left ventricular blood supply."}
{"id": "PMID:1079485", "title": "Prinzmetal's variant form of angina pectoris. Re-evaluation of mechanisms.", "content": "Thirty-five patients with typical Prinzmetal's variant angina were studied by coronary cineangiography. There was no demonstrable stenosis of the major coronary arteries in 19 patients. Nine patients with single coronary stenosis underwent aortocoronary bypass and had recurrence of the symptoms postoperatively. Administration of nifedipine effected complete cessation of the symptoms among patients formerly treated medically. Although surgical treatment did not effect permanent relief of pain, all patients initially treated surgically experienced relief of pain when nifedipine was administered. The pathophysiology of variant angina remains obscure. Our results suggest that neurohumoral factors exert more of an effect on the myocardial cell than on the coronary vessels.", "contents": "Prinzmetal's variant form of angina pectoris. Re-evaluation of mechanisms. Thirty-five patients with typical Prinzmetal's variant angina were studied by coronary cineangiography. There was no demonstrable stenosis of the major coronary arteries in 19 patients. Nine patients with single coronary stenosis underwent aortocoronary bypass and had recurrence of the symptoms postoperatively. Administration of nifedipine effected complete cessation of the symptoms among patients formerly treated medically. Although surgical treatment did not effect permanent relief of pain, all patients initially treated surgically experienced relief of pain when nifedipine was administered. The pathophysiology of variant angina remains obscure. Our results suggest that neurohumoral factors exert more of an effect on the myocardial cell than on the coronary vessels."}
{"id": "PMID:1079486", "title": "Regional myocardial blood flow measurements before and after coronary bypass surgery. A preliminary report.", "content": "Myocardial blood flow in 16 patients before and after coronary bypass surgery in conjunction with coronary angiography, left ventricular function measurement, and graded exercise test. Radioactive 133-Xe was injected into the coronary artery or bypass graft and the washout was recorded by an Anger camera. Myocardial blood flow increased in 11 out of 14 patients and decreased in three patients. The average flow was 55 (plus or minus 6) preoperatively and 96 (plus or minus 10) ml 100 g/min postoperatively. Increase of blood flow occurred in both the bypassed left anterior descending coronary artery (LAD) region and the nonbypassed left circumflex coronary artery (LCIR) region. The postoperative flow increase and the absolute postoperative flow values are higher with saphenous vein than with mammary artery grafts. Statistically significant correlation is not found between myocardial blood flow changes and exerice tolerance. The volume measurements (end diastolic volume, stroke volume, ejection fraction) remained unchanged.", "contents": "Regional myocardial blood flow measurements before and after coronary bypass surgery. A preliminary report. Myocardial blood flow in 16 patients before and after coronary bypass surgery in conjunction with coronary angiography, left ventricular function measurement, and graded exercise test. Radioactive 133-Xe was injected into the coronary artery or bypass graft and the washout was recorded by an Anger camera. Myocardial blood flow increased in 11 out of 14 patients and decreased in three patients. The average flow was 55 (plus or minus 6) preoperatively and 96 (plus or minus 10) ml 100 g/min postoperatively. Increase of blood flow occurred in both the bypassed left anterior descending coronary artery (LAD) region and the nonbypassed left circumflex coronary artery (LCIR) region. The postoperative flow increase and the absolute postoperative flow values are higher with saphenous vein than with mammary artery grafts. Statistically significant correlation is not found between myocardial blood flow changes and exerice tolerance. The volume measurements (end diastolic volume, stroke volume, ejection fraction) remained unchanged."}
{"id": "PMID:1079489", "title": "[Haemodialysis for renal failure in a case of systemic lupus erythematosus (author's transl)].", "content": "Severe acute renal failure developed in a patient with systemic lupus erythematosus which was characterized by typical skin rash, polyserositis, haemolytic anaemia and liver damage. Serological examination revealed anti-DNA antibodies in high concentrations, positive Coombs' test, excessive elevation of serum-IgG with markedly depressed serum complement fractions (C3, C4). Acute lupus nephritis led to prolonged oliguria and anuria requiring regular dialysis over almost five months. Immuno-suppressive treatment with cortisone and azathioprine finally reversed the renal failure: BUN returned to 33 mg/  100 ml, with a plasma creatinine of 1.8 mg per 100 ml.", "contents": "[Haemodialysis for renal failure in a case of systemic lupus erythematosus (author's transl)]. Severe acute renal failure developed in a patient with systemic lupus erythematosus which was characterized by typical skin rash, polyserositis, haemolytic anaemia and liver damage. Serological examination revealed anti-DNA antibodies in high concentrations, positive Coombs' test, excessive elevation of serum-IgG with markedly depressed serum complement fractions (C3, C4). Acute lupus nephritis led to prolonged oliguria and anuria requiring regular dialysis over almost five months. Immuno-suppressive treatment with cortisone and azathioprine finally reversed the renal failure: BUN returned to 33 mg/  100 ml, with a plasma creatinine of 1.8 mg per 100 ml."}
{"id": "PMID:1079490", "title": "[Immuno-coagulopathies and their dangers in dental-surgical interventions].", "content": "It is reported on three patients with an immunocoagulopathy, the diagnosis of which was established on the basis of immunological coagulation studies.  These patients were submitted to oral surgery.  In connection with the case histories, it is reported on pathogenesis, diagnostics, and special therapeutical requirements in cases of hemorrhagic diathesis with a circulating anticoagulant antibody.", "contents": "[Immuno-coagulopathies and their dangers in dental-surgical interventions]. It is reported on three patients with an immunocoagulopathy, the diagnosis of which was established on the basis of immunological coagulation studies.  These patients were submitted to oral surgery.  In connection with the case histories, it is reported on pathogenesis, diagnostics, and special therapeutical requirements in cases of hemorrhagic diathesis with a circulating anticoagulant antibody."}
{"id": "PMID:1079495", "title": "[Influence of vestibular and afferent impulses on the spontaneous activity of interneurons in the spinal cord of cats].", "content": "Responses of the cat spinal interneurons were studied under electrical stimulation of dorsal roots and adequate stimulation of vestibular apparatus.  The evoked activity of interneurons in response to electrical stimulation of dorsal roots had different forms.  Three types of responses to adequate stimulation of vestibular apparatus were observed.  The patterns of convergence of afferent and vestibular impulses on the interneurons are discussed.", "contents": "[Influence of vestibular and afferent impulses on the spontaneous activity of interneurons in the spinal cord of cats]. Responses of the cat spinal interneurons were studied under electrical stimulation of dorsal roots and adequate stimulation of vestibular apparatus.  The evoked activity of interneurons in response to electrical stimulation of dorsal roots had different forms.  Three types of responses to adequate stimulation of vestibular apparatus were observed.  The patterns of convergence of afferent and vestibular impulses on the interneurons are discussed."}
{"id": "PMID:1079496", "title": "CSF scanning in achondroplastic children with cranial enlargement.", "content": "This paper reports on an 18-month-old achondroplastic child with dilated lateral ventricles.  Results of a cisternographic investigation were normal.  No shunting procedure was carried out, and subsequent clinical progress was satisfactory.  Some patho-physiological aspects of achondroplastic dysgenesis are discussed.", "contents": "CSF scanning in achondroplastic children with cranial enlargement. This paper reports on an 18-month-old achondroplastic child with dilated lateral ventricles.  Results of a cisternographic investigation were normal.  No shunting procedure was carried out, and subsequent clinical progress was satisfactory.  Some patho-physiological aspects of achondroplastic dysgenesis are discussed."}
{"id": "PMID:1079509", "title": "Paracortical alteration in lymphadenopathic and tumor-draining lymph nodes: histologic study.", "content": "Histologic studies of 142 enlarged lymph nodes from 124 patients, diagnosed histologically as nonspecific chronic lymphadenitis or reactive hyperplasia, revealed a characteristic nodular alteration in the paracortical area of nodes from the axillary, cervical, and inguinal regions, and rarely in abdominal, mediastinal, and retroperitoneal groups. In 1389 lymph nodes from 100 radical mastectomy specimens similar changes were noted more frequently in the lower than in higher levels of the tumor-draining axillary nodes. The nodule was composed of small lymphocytes associated with postcapillary venules and histiocytes-macrophages. The latter varied in distribution from a scattered pattern resembling a starry-sky appearance in the initial phase of paracortical alteration to the replacement of small lymphocytes in advanced stages. Plasma cells and lymphatic sinuses were characteristically absent from this nodular alteration. An impressive example of such changes was seen in four cases of dermatopathic lymphadenitis. Studies of the paracortical area may provide new insight into the pathogenesis of some lymphadenopathies.", "contents": "Paracortical alteration in lymphadenopathic and tumor-draining lymph nodes: histologic study. Histologic studies of 142 enlarged lymph nodes from 124 patients, diagnosed histologically as nonspecific chronic lymphadenitis or reactive hyperplasia, revealed a characteristic nodular alteration in the paracortical area of nodes from the axillary, cervical, and inguinal regions, and rarely in abdominal, mediastinal, and retroperitoneal groups. In 1389 lymph nodes from 100 radical mastectomy specimens similar changes were noted more frequently in the lower than in higher levels of the tumor-draining axillary nodes. The nodule was composed of small lymphocytes associated with postcapillary venules and histiocytes-macrophages. The latter varied in distribution from a scattered pattern resembling a starry-sky appearance in the initial phase of paracortical alteration to the replacement of small lymphocytes in advanced stages. Plasma cells and lymphatic sinuses were characteristically absent from this nodular alteration. An impressive example of such changes was seen in four cases of dermatopathic lymphadenitis. Studies of the paracortical area may provide new insight into the pathogenesis of some lymphadenopathies."}
{"id": "PMID:1079510", "title": "Tolerance induction in TxXBT and TxXB mice.", "content": "Adult normal mice and those mice which had been thymectomized, X-irradiated, and reconstituted either with bone marrow cells and thymus cells (TxXBT) or with bone marrow cells along (TxXB), were given varying amounts (0.1-5 mg) of deaggregated soluble bovine serum albumin (sBSA). They were challenged 10 days later with an immunogenic form of BSA. TxXB mice were supplemented with normal thymus cells 3 days before the challenge. With any dose of sBSA, TxXBT and normal mice were made tolerant. Only 5 mg of sBSA, the highest dose in these experiments, was effective in inducing the tolerance in TxXB mice. The simultaneous administration of 5 mg of sBSA and 0.1 mg of alumprecipitated BSA plus 0.01 mg of endotoxin resulted in the priming in TxXBT mice but induced tolerance in TxXB mice. These results indicate that: (a) B-cell tolerance could be induced independently of the influence of T cells; (b) the tolerogen susceptibility of B cells may be lower than that of T cells; (c) such a weakly immunogenic agent as the mixture of tolerogen and immunogen could either activate the antibody response in the presence of T cells or induce B-cell tolerance in the absence of T cells.", "contents": "Tolerance induction in TxXBT and TxXB mice. Adult normal mice and those mice which had been thymectomized, X-irradiated, and reconstituted either with bone marrow cells and thymus cells (TxXBT) or with bone marrow cells along (TxXB), were given varying amounts (0.1-5 mg) of deaggregated soluble bovine serum albumin (sBSA). They were challenged 10 days later with an immunogenic form of BSA. TxXB mice were supplemented with normal thymus cells 3 days before the challenge. With any dose of sBSA, TxXBT and normal mice were made tolerant. Only 5 mg of sBSA, the highest dose in these experiments, was effective in inducing the tolerance in TxXB mice. The simultaneous administration of 5 mg of sBSA and 0.1 mg of alumprecipitated BSA plus 0.01 mg of endotoxin resulted in the priming in TxXBT mice but induced tolerance in TxXB mice. These results indicate that: (a) B-cell tolerance could be induced independently of the influence of T cells; (b) the tolerogen susceptibility of B cells may be lower than that of T cells; (c) such a weakly immunogenic agent as the mixture of tolerogen and immunogen could either activate the antibody response in the presence of T cells or induce B-cell tolerance in the absence of T cells."}
{"id": "PMID:1079511", "title": "Limiting dilution analysis of helper T-cell function.", "content": "Limiting dilution analysis has been applied to the study of T-cell 'helper' function in vitro. Using the microculture system one can estimate the numbers of (a) 'helper' T cells involved in specific collaboration with B cells and (b) those T cells which are able, on being activated by their specific antigen, to facilitate the response of B cells to another antigen. Such studies have enabled us to demonstrate that: (1) a single 'helper' T cell was able to activate a single B-cell precursor to detectable antibody production; (2) the 'helper' function of primed T cells was radio-resistant; (3) a minimal estimate of 'helper' frequencies could be obtained in defined cell populations; (4) nonspecific facilitation was directed towards virtually all available B cells of a given specificity if these were challenged with their appropriate particulate antigen; (5) the microculture system offers the opportunity to determine whether specific and non-specific T-cell 'helper' effects are a consequence of the activity of one T-cell type or of differenct subpopulations of T cells.", "contents": "Limiting dilution analysis of helper T-cell function. Limiting dilution analysis has been applied to the study of T-cell 'helper' function in vitro. Using the microculture system one can estimate the numbers of (a) 'helper' T cells involved in specific collaboration with B cells and (b) those T cells which are able, on being activated by their specific antigen, to facilitate the response of B cells to another antigen. Such studies have enabled us to demonstrate that: (1) a single 'helper' T cell was able to activate a single B-cell precursor to detectable antibody production; (2) the 'helper' function of primed T cells was radio-resistant; (3) a minimal estimate of 'helper' frequencies could be obtained in defined cell populations; (4) nonspecific facilitation was directed towards virtually all available B cells of a given specificity if these were challenged with their appropriate particulate antigen; (5) the microculture system offers the opportunity to determine whether specific and non-specific T-cell 'helper' effects are a consequence of the activity of one T-cell type or of differenct subpopulations of T cells."}
{"id": "PMID:1079514", "title": "An investigation of the characteristics of a negative pion beam by means of induced chromosome aberration in human peripheral blood lymphocytes.", "content": "The depth dose profiles of unstable chromosome aberrations were determined by irradiating blood samples at various depths in a plastic phantom exposed to a beam of negative pi mesons (pions). The pions were derived from an experimental beam line from the Nimrod 7 GeV proton synchrotron. With a momentum of 160 MeV/c and a spread of 15 per cent a Bragg profile was produced peaking at 13-9 cm. An ionization peak to plateau ratio of 1-6 was obtained and for cytogenetic damage this was increased to 2-9. Dicentric and acentric dose-response data from peak and plateau positions were fitted to Y=alphaD+betaD-2 models for comparison with data obtained from 60-Co gamma-rays and fission neutrons. With respect to 150 rads gamma-radiation, the r.b.e. values for the pion peak were 2-1 for dicentrics and 2-3 for acentrics. In the plateau the value for both aberrations was 1-5. At pion doses likely to be used as daily radiotherapy fractions, the aberrations predominantly resulted from single-track events, indicating little likelihood of interfraction recovery, particularly in the peak position. It is concluded that the biological damage characteristics of pion beams, as determined from a cytogenetic end-point, offer several features attractive to the radiotherapist.", "contents": "An investigation of the characteristics of a negative pion beam by means of induced chromosome aberration in human peripheral blood lymphocytes. The depth dose profiles of unstable chromosome aberrations were determined by irradiating blood samples at various depths in a plastic phantom exposed to a beam of negative pi mesons (pions). The pions were derived from an experimental beam line from the Nimrod 7 GeV proton synchrotron. With a momentum of 160 MeV/c and a spread of 15 per cent a Bragg profile was produced peaking at 13-9 cm. An ionization peak to plateau ratio of 1-6 was obtained and for cytogenetic damage this was increased to 2-9. Dicentric and acentric dose-response data from peak and plateau positions were fitted to Y=alphaD+betaD-2 models for comparison with data obtained from 60-Co gamma-rays and fission neutrons. With respect to 150 rads gamma-radiation, the r.b.e. values for the pion peak were 2-1 for dicentrics and 2-3 for acentrics. In the plateau the value for both aberrations was 1-5. At pion doses likely to be used as daily radiotherapy fractions, the aberrations predominantly resulted from single-track events, indicating little likelihood of interfraction recovery, particularly in the peak position. It is concluded that the biological damage characteristics of pion beams, as determined from a cytogenetic end-point, offer several features attractive to the radiotherapist."}
{"id": "PMID:1079515", "title": "Inhibitory effect of U.V. -irradiation on the formation of twisted circular lambda DNA.", "content": "Ultra-violet irradiation of lambda phage results in an inhibition of conversion of lambda DNA in host cells from the initial linear form to twisted circular form. The formation of open circular lambda DNA in vitro (formation of Hershey's circle) was quite resistant to the irradiation. The occurrence of denaturation of ultraviolet irradiation was detected in lambda DNA.", "contents": "Inhibitory effect of U.V. -irradiation on the formation of twisted circular lambda DNA. Ultra-violet irradiation of lambda phage results in an inhibition of conversion of lambda DNA in host cells from the initial linear form to twisted circular form. The formation of open circular lambda DNA in vitro (formation of Hershey's circle) was quite resistant to the irradiation. The occurrence of denaturation of ultraviolet irradiation was detected in lambda DNA."}
{"id": "PMID:1079516", "title": "Maximum r.b.e. of fission neutrons for induction of somatic mutations in maize.", "content": "This investigation was conducted to determine the maximum r.b.e. of fission neutrons for the induction of yellow-green sectors in maize. Seeds heterozygous for the yg2 locus were adjusted to 13 per cent water content, irradiated with varying doses for eitherfission neutrons or 60Co gamma-radiation, and soaked for 2 hours in nitrogen-bubbled water at ambient temperature before planting. Frequency of yg2 sectors, as scored in leaves 4 and 5, increase linearly for both radiations. The r.b.e. values were greater than1100 at 0.40 sectors per leaf 4 and greater than 600 at 0.20 sectors per leaf 5; however, at these low frequencies, the standard errors were  3 times greater than the r.b.e. At 6.0 sectors per leaf 4, the r.b.e. values were 111 plus or minus 9; and at 3.0sectors per leaf 5, 118 plus or minus 21. As measured by sectors per krad, mutagenic efficiency increased with increased dose of gamma-radiation; the opposite was true for fission neutrons. This trend was more evident for leaf 4 than for leaf 5.", "contents": "Maximum r.b.e. of fission neutrons for induction of somatic mutations in maize. This investigation was conducted to determine the maximum r.b.e. of fission neutrons for the induction of yellow-green sectors in maize. Seeds heterozygous for the yg2 locus were adjusted to 13 per cent water content, irradiated with varying doses for eitherfission neutrons or 60Co gamma-radiation, and soaked for 2 hours in nitrogen-bubbled water at ambient temperature before planting. Frequency of yg2 sectors, as scored in leaves 4 and 5, increase linearly for both radiations. The r.b.e. values were greater than1100 at 0.40 sectors per leaf 4 and greater than 600 at 0.20 sectors per leaf 5; however, at these low frequencies, the standard errors were  3 times greater than the r.b.e. At 6.0 sectors per leaf 4, the r.b.e. values were 111 plus or minus 9; and at 3.0sectors per leaf 5, 118 plus or minus 21. As measured by sectors per krad, mutagenic efficiency increased with increased dose of gamma-radiation; the opposite was true for fission neutrons. This trend was more evident for leaf 4 than for leaf 5."}
{"id": "PMID:1079520", "title": "Vasopressin and hepatic artery.  Effect of selective celiac infusion of vasopressin on the hepatic artery flow.", "content": "Electromagnetic flow measurement study was performed in 20 anesthetized dogs to evaluate the effect of selective celiac infusion of vasopressin on the hepatic arterial vasculature.  Teh hepatic arterial flow showed a biphasic response with an initial decrease followed by a substantial increase in spite of a continued infusion.  The left gastric, splenic, and superior mesentric arteries showed a monophasic response with persistent decrease of flow during the whole infusion.  The biphasic response of the hepatic arterial flow is thought to be due to autoregulatory dilative action of the liver to a decrease of the portal flow.  The results and previous clinical experience suggest that the selective infusions of vasopressin into arteries supplying the liver can be used for short-term vasoconstrictive therapy of acute gastrointestinal bleeding in patients without liver damage.  Further experience is necessary to evaluate the safety of prolonged hepatic infusions in patients with liver damage.", "contents": "Vasopressin and hepatic artery.  Effect of selective celiac infusion of vasopressin on the hepatic artery flow. Electromagnetic flow measurement study was performed in 20 anesthetized dogs to evaluate the effect of selective celiac infusion of vasopressin on the hepatic arterial vasculature.  Teh hepatic arterial flow showed a biphasic response with an initial decrease followed by a substantial increase in spite of a continued infusion.  The left gastric, splenic, and superior mesentric arteries showed a monophasic response with persistent decrease of flow during the whole infusion.  The biphasic response of the hepatic arterial flow is thought to be due to autoregulatory dilative action of the liver to a decrease of the portal flow.  The results and previous clinical experience suggest that the selective infusions of vasopressin into arteries supplying the liver can be used for short-term vasoconstrictive therapy of acute gastrointestinal bleeding in patients without liver damage.  Further experience is necessary to evaluate the safety of prolonged hepatic infusions in patients with liver damage."}
{"id": "PMID:1079521", "title": "Experimental catheter obstruction of the gastric coronary vein.  Possible technique for percutaneous intravascular tamponade of the gastroesophageal varices.", "content": "Experimental catheter obstruction of the left gastric (coronary) vein was explored in 10 dogs.  Using a transjugular approach, the liver was punctured and the portal vein catheterized.  Coaxially introduced catheters were then used to catheterize selectively and produce an intravascular obstruction (tamponade) of the gastric coronary vein.  Ballon catheters were used for a temporary occlusion.  Injection of a tissue adhesive, isobutyl 2-cyanoacrylate, was used for a definitive obliteration.  The achieved results show the anatomical feasibility of this approach and give good perspective for development of a clinical method for percutaneous intravascular tamponade of the bleeding gastroesophageal varices in cirrhotics.", "contents": "Experimental catheter obstruction of the gastric coronary vein.  Possible technique for percutaneous intravascular tamponade of the gastroesophageal varices. Experimental catheter obstruction of the left gastric (coronary) vein was explored in 10 dogs.  Using a transjugular approach, the liver was punctured and the portal vein catheterized.  Coaxially introduced catheters were then used to catheterize selectively and produce an intravascular obstruction (tamponade) of the gastric coronary vein.  Ballon catheters were used for a temporary occlusion.  Injection of a tissue adhesive, isobutyl 2-cyanoacrylate, was used for a definitive obliteration.  The achieved results show the anatomical feasibility of this approach and give good perspective for development of a clinical method for percutaneous intravascular tamponade of the bleeding gastroesophageal varices in cirrhotics."}
{"id": "PMID:1079522", "title": "Comparison of iothalamate meglumine, metrizamide and iodophendylate in cerebral ventriculography.  A clinical, radiological and histopathological study in the rat.", "content": "Clinical, radiological and histopathological findings following cerebral ventriculography in the rat using the water-soluble contrast media iothalamate meglumine and metrizamide, and the oil-soluble iodophendylate are reported.  Clinically, iothalamate meglumine caused convulsions, while there were no adverse reactions to the other media.  Radiologically there was good visualizaiton of the ventricles with all media.  Iodophendylate was retained in the ventricles for the duration of the experiment (up to 60 days), while the water-soluble media had disappeared within 20 minutes.  Histologically there were no pathological changes attributable to the water-soluble media.  Iodophendyalte led to enlargement of the ventricles, and macrophages containing oil were seen on the ependymal lining.", "contents": "Comparison of iothalamate meglumine, metrizamide and iodophendylate in cerebral ventriculography.  A clinical, radiological and histopathological study in the rat. Clinical, radiological and histopathological findings following cerebral ventriculography in the rat using the water-soluble contrast media iothalamate meglumine and metrizamide, and the oil-soluble iodophendylate are reported.  Clinically, iothalamate meglumine caused convulsions, while there were no adverse reactions to the other media.  Radiologically there was good visualizaiton of the ventricles with all media.  Iodophendylate was retained in the ventricles for the duration of the experiment (up to 60 days), while the water-soluble media had disappeared within 20 minutes.  Histologically there were no pathological changes attributable to the water-soluble media.  Iodophendyalte led to enlargement of the ventricles, and macrophages containing oil were seen on the ependymal lining."}
{"id": "PMID:1079524", "title": "A symposium on surgery for coronary artery disease. Patient selection and preoperative evaluation.", "content": "Patient selection based upon preoperative evaluation is a complex problem. In using a procedure with the tremenduous implications and applications seen in aortocoronary bypass grafting, excesses in enthusiasm and criticism are the rule rather than the exception. With patience and careful evaluation,,the correct place for the procedure will be found and, though it is not quite the panacea once claimed for patients with coronary artery disease, aortocoronary bypass surgery will remain an important and valuable therapeutic tool, perhaps the most significant development in cardiovascular treatment of the past decade.", "contents": "A symposium on surgery for coronary artery disease. Patient selection and preoperative evaluation. Patient selection based upon preoperative evaluation is a complex problem. In using a procedure with the tremenduous implications and applications seen in aortocoronary bypass grafting, excesses in enthusiasm and criticism are the rule rather than the exception. With patience and careful evaluation,,the correct place for the procedure will be found and, though it is not quite the panacea once claimed for patients with coronary artery disease, aortocoronary bypass surgery will remain an important and valuable therapeutic tool, perhaps the most significant development in cardiovascular treatment of the past decade."}
{"id": "PMID:1079525", "title": "Aortocoronary bypass-classification and results.", "content": "Technical, physiologic, metabolic, and pathologic factors combine to determine the long-term patency of aortocoronary bypass grafts. Numerous variables are present in each of these categories, and the results of the operation cannot be determined unless comparable groups of patients are studied objectively. The classification proposed in this paper takes into account many of the factors that can effect graft patency. The available results of direct coronary arterial surgery are stated and used as the basis for five-year projections in the context of the proposed classification system.", "contents": "Aortocoronary bypass-classification and results. Technical, physiologic, metabolic, and pathologic factors combine to determine the long-term patency of aortocoronary bypass grafts. Numerous variables are present in each of these categories, and the results of the operation cannot be determined unless comparable groups of patients are studied objectively. The classification proposed in this paper takes into account many of the factors that can effect graft patency. The available results of direct coronary arterial surgery are stated and used as the basis for five-year projections in the context of the proposed classification system."}
{"id": "PMID:1079527", "title": "Low free serum histidine concentration in rheumatoid arthritis. A measure of disease activity.", "content": "A study of sera from 285 patients with definite or classical rheumatoid arthritis (including 37 patients receiving no anti-inflammatory drugs) and sera from 67 healthy subjects has confirmed 10 published reports of a statistically significant decreased blood histidine concentration in patients with rheumatoid arthritis. Contrastingly, in sera from 231 patients with a variety of acute and chronic illnesses other than rheumatoid arthritis, no statistically significant hypohistidinemia was observed either in the group as a whole or in association with the administration of aspirin, prednisone, indomethacin, phenylbutazone, or dextropropoxyphene. In the patients with rheumatoid arthritis there was a statistically significant correlation between the serum histidine concentration and the following: Westergren sedimentation rate (r=-0.33, P smaller than 10- minus 9), grip strength (r=0.26, P smaller than 10- minus 9), hematocrit (r=0.23, P smaller than 10- minus 9), duration of morning stiffness (r=-0.14, P=10- minus 5), walking time (r=-0.13, P=10- minus 4), latex titer of rheumatoid factor (r=-0.11, P=0.001), and the duration of arthritis (r=-0.06, P=0.05). There was no statistically significant association between the serum histidine concentration and the duration of rheumatoid arthritis in the 151 patients with disease of 0-10-yr duration (r=0.02, P=0.5), the sex of the patient, or the presence of antinuclear antibody (R=0.007, P=0.9). The serum histidine concentration was less in rheumatoid patients receiving steroids (P=0.00001), gold (P=0.009), and aspirin (P=0.15) than in rheumatoid patients not receiving these drugs. This study indicates that histidine determinations on properly preserved casual serum samples can be helpful in the diagnosis of rheumatoid arthritis and in the evaluation of the activity of the disease.", "contents": "Low free serum histidine concentration in rheumatoid arthritis. A measure of disease activity. A study of sera from 285 patients with definite or classical rheumatoid arthritis (including 37 patients receiving no anti-inflammatory drugs) and sera from 67 healthy subjects has confirmed 10 published reports of a statistically significant decreased blood histidine concentration in patients with rheumatoid arthritis. Contrastingly, in sera from 231 patients with a variety of acute and chronic illnesses other than rheumatoid arthritis, no statistically significant hypohistidinemia was observed either in the group as a whole or in association with the administration of aspirin, prednisone, indomethacin, phenylbutazone, or dextropropoxyphene. In the patients with rheumatoid arthritis there was a statistically significant correlation between the serum histidine concentration and the following: Westergren sedimentation rate (r=-0.33, P smaller than 10- minus 9), grip strength (r=0.26, P smaller than 10- minus 9), hematocrit (r=0.23, P smaller than 10- minus 9), duration of morning stiffness (r=-0.14, P=10- minus 5), walking time (r=-0.13, P=10- minus 4), latex titer of rheumatoid factor (r=-0.11, P=0.001), and the duration of arthritis (r=-0.06, P=0.05). There was no statistically significant association between the serum histidine concentration and the duration of rheumatoid arthritis in the 151 patients with disease of 0-10-yr duration (r=0.02, P=0.5), the sex of the patient, or the presence of antinuclear antibody (R=0.007, P=0.9). The serum histidine concentration was less in rheumatoid patients receiving steroids (P=0.00001), gold (P=0.009), and aspirin (P=0.15) than in rheumatoid patients not receiving these drugs. This study indicates that histidine determinations on properly preserved casual serum samples can be helpful in the diagnosis of rheumatoid arthritis and in the evaluation of the activity of the disease."}
{"id": "PMID:1079526", "title": "Gas endarterectomy with distal bypass of the coronary arteries.", "content": "Carbon dioxide gas endarterectomy was first applied in cases of total occlusion of the right coronary artery. The early technique involved removal of the disease core along the entire length of the vessel. At follow-up, progressive changes were observed to cause segmental stenosis within the proximal and middle portions of the vessel which led to complete reocclusion of the artery at 36 months. In every case, however, the distal branches of the endarterectomized arteries appeared unaffected. On the basis of these results, we have modified our technique. The present treatment involves distal endarterectomy with aortocoronary bypass to the endarterectomized segment. This surgical approach is appropriated only in cases of diffuse atherosclerotic involvement of the distal arterial branches. It can be applied to any one of the three main coronary artery branches and has therefore broadened the range of patient selection for direct myocardial revascularization. The operative mortality in our series of 58 patients was 6.9 per cent (4 patients), but all deaths were associated with such aggravating factors as anerurysm formation, valve disease, and the preoperative evolution of myocardial infarction. The associated morbidity rate was 6.8 per cent; two patients had disruption of the sternal wound, and one suffered uncomplicated myocardial infarction. The fourth patient had a myocardial infarction followed by development of conduction defect and required operation for the insertion of a permanent transvenous pacemaker. To date, follow-up indicates a high patency rate among the surviving 54 patients.", "contents": "Gas endarterectomy with distal bypass of the coronary arteries. Carbon dioxide gas endarterectomy was first applied in cases of total occlusion of the right coronary artery. The early technique involved removal of the disease core along the entire length of the vessel. At follow-up, progressive changes were observed to cause segmental stenosis within the proximal and middle portions of the vessel which led to complete reocclusion of the artery at 36 months. In every case, however, the distal branches of the endarterectomized arteries appeared unaffected. On the basis of these results, we have modified our technique. The present treatment involves distal endarterectomy with aortocoronary bypass to the endarterectomized segment. This surgical approach is appropriated only in cases of diffuse atherosclerotic involvement of the distal arterial branches. It can be applied to any one of the three main coronary artery branches and has therefore broadened the range of patient selection for direct myocardial revascularization. The operative mortality in our series of 58 patients was 6.9 per cent (4 patients), but all deaths were associated with such aggravating factors as anerurysm formation, valve disease, and the preoperative evolution of myocardial infarction. The associated morbidity rate was 6.8 per cent; two patients had disruption of the sternal wound, and one suffered uncomplicated myocardial infarction. The fourth patient had a myocardial infarction followed by development of conduction defect and required operation for the insertion of a permanent transvenous pacemaker. To date, follow-up indicates a high patency rate among the surviving 54 patients."}
{"id": "PMID:1079529", "title": "Mouse embryo development in vitro: effects of inhibitors of RNA and protein synthesis on blastocyst and post-blastocyst embryos.", "content": "The effect of inhibitors of RNA synthesis (Cordycepin, Actinomycin D) and protein synthesis (Cycloheximide) on the development and growth of mouse blastocysts in vitro was explored. Blastocysts exposed in vitro for 24 hours to 50 mu-g/ml Cordycepin, 0.005 mu-g/ml Actinomycin D, or 0.1 mu-g/ml Cycloheximde grew and began to attach to the dish in the similar manner as did the controls. Cell number, 3-H-thymidine-labeling index and mitotic index in treated blastocysts were also similar to controls. Cell number, 3-H-thymidine-labeling index and mitotic index in treated blastocysts were also similar to controls. Control blastocysts grown in vitro for six days attached to the dish, trophoblastic layer was spread and inner cell mass continued to grow and formed an egg-cylinder like structure. Blastocysts grown in constant presence of 50 mu-g/ml of Cordycepin in themedium or those exposed to inhibitor only for the first 24 hours failed to develop inner cell mass derivatives in culture, although the growth of trophoblastic cells was as in controls. The same results were obtained if blastocysts were exposed to 0.005 mu-g/ml of Actinomycin D or to 0.1 mu-g/ml of Cycloheximide either continuously or for the first 24 hours. Higher concentrations of Actinomycin D (0.05 mu-g/ml) or Cycloheximde (1 mu-g/ml) were toxic for the blastocysts causing their degeneration within 24-48 hours. Our results suggested that appropriate concentrations of RNA or protein synthesis inhibitors could prevent the development of inner cell mass derivatives with essentially no effect on the development of primary trophoblast. This would indicate that the process of differentiation of inner cell mass cells is much more sensitive to metabolic inhibitors than the differentiation of giant trophoblastic cells.", "contents": "Mouse embryo development in vitro: effects of inhibitors of RNA and protein synthesis on blastocyst and post-blastocyst embryos. The effect of inhibitors of RNA synthesis (Cordycepin, Actinomycin D) and protein synthesis (Cycloheximide) on the development and growth of mouse blastocysts in vitro was explored. Blastocysts exposed in vitro for 24 hours to 50 mu-g/ml Cordycepin, 0.005 mu-g/ml Actinomycin D, or 0.1 mu-g/ml Cycloheximde grew and began to attach to the dish in the similar manner as did the controls. Cell number, 3-H-thymidine-labeling index and mitotic index in treated blastocysts were also similar to controls. Cell number, 3-H-thymidine-labeling index and mitotic index in treated blastocysts were also similar to controls. Control blastocysts grown in vitro for six days attached to the dish, trophoblastic layer was spread and inner cell mass continued to grow and formed an egg-cylinder like structure. Blastocysts grown in constant presence of 50 mu-g/ml of Cordycepin in themedium or those exposed to inhibitor only for the first 24 hours failed to develop inner cell mass derivatives in culture, although the growth of trophoblastic cells was as in controls. The same results were obtained if blastocysts were exposed to 0.005 mu-g/ml of Actinomycin D or to 0.1 mu-g/ml of Cycloheximide either continuously or for the first 24 hours. Higher concentrations of Actinomycin D (0.05 mu-g/ml) or Cycloheximde (1 mu-g/ml) were toxic for the blastocysts causing their degeneration within 24-48 hours. Our results suggested that appropriate concentrations of RNA or protein synthesis inhibitors could prevent the development of inner cell mass derivatives with essentially no effect on the development of primary trophoblast. This would indicate that the process of differentiation of inner cell mass cells is much more sensitive to metabolic inhibitors than the differentiation of giant trophoblastic cells."}
{"id": "PMID:1079533", "title": "Azulene analogs of pharmacological agents III: acute toxicity and local anesthetic activity of azulylamides and azulenecarboxamides.", "content": "This paper describes the acute toxicity of two azulylamides and six azulenecarboxamides and the nerve conduction-inhibiting properties of some of these compounds. The azulene derivatives are compared to their benzenoid prototypes, lidocaine and procainamide, as part of a continuing investigation of the biological properties of nonbenzenoid aromatic compounds.", "contents": "Azulene analogs of pharmacological agents III: acute toxicity and local anesthetic activity of azulylamides and azulenecarboxamides. This paper describes the acute toxicity of two azulylamides and six azulenecarboxamides and the nerve conduction-inhibiting properties of some of these compounds. The azulene derivatives are compared to their benzenoid prototypes, lidocaine and procainamide, as part of a continuing investigation of the biological properties of nonbenzenoid aromatic compounds."}
{"id": "PMID:1079534", "title": "Mechanical deactivation induced by active shortening in isolated muscle fibres of the frog.", "content": "1. The effect of active shortening on the time course and magnitude of isometric tension development during a single twitch and during an incompletely fused tetanus was studied at 0-2-1-2 degres C in isolated semitendinosus muscle fibres of the frog. 2. Active shortening caused a depression of the contractile force without markedly affecting the total duration of the twitch. The depressant effect increased with increasing amounts of sarcomere shortening. Sarcomere shortenings of 0-05 mum and 0-3 mum reduced the twitch force by approximately 5 and 20 percent of the maximal tetanic tension, respectively. 3. A given sarcomere shortening induced the same absolute amount of depression of the contractile strength when the movement was carried out at different times during the initial 200-250 msec after the stimulus. 4. The influence of load and velocity of shortening during the movement phase was studied. Differences in load ranging between zero and 1/3 of the maximal tetanic tension (with concomitant changes in speed of shortening from Vmax to approximately 1/5 of Vmax) did not affect the degree of depression markedly. Underthe conditions studied, the extent of movement appeared to be the only significant determinant of the depressant effect. 5. The reduction in force induced by active shortening persisted for 800-900 msec during an incompletely fused tetanus. 6. It is suggested that the depressant effect is based on a structural change in the myofilament system that is produced as the A and I filaments slide along each other during muscle activity.", "contents": "Mechanical deactivation induced by active shortening in isolated muscle fibres of the frog. 1. The effect of active shortening on the time course and magnitude of isometric tension development during a single twitch and during an incompletely fused tetanus was studied at 0-2-1-2 degres C in isolated semitendinosus muscle fibres of the frog. 2. Active shortening caused a depression of the contractile force without markedly affecting the total duration of the twitch. The depressant effect increased with increasing amounts of sarcomere shortening. Sarcomere shortenings of 0-05 mum and 0-3 mum reduced the twitch force by approximately 5 and 20 percent of the maximal tetanic tension, respectively. 3. A given sarcomere shortening induced the same absolute amount of depression of the contractile strength when the movement was carried out at different times during the initial 200-250 msec after the stimulus. 4. The influence of load and velocity of shortening during the movement phase was studied. Differences in load ranging between zero and 1/3 of the maximal tetanic tension (with concomitant changes in speed of shortening from Vmax to approximately 1/5 of Vmax) did not affect the degree of depression markedly. Underthe conditions studied, the extent of movement appeared to be the only significant determinant of the depressant effect. 5. The reduction in force induced by active shortening persisted for 800-900 msec during an incompletely fused tetanus. 6. It is suggested that the depressant effect is based on a structural change in the myofilament system that is produced as the A and I filaments slide along each other during muscle activity."}
{"id": "PMID:1079535", "title": "Receptive field organization of ganglion cells in the frog retina: contributions from cones, green rods and red rods.", "content": "1. The impulse discharge of ganglion cells was recorded with extracellular micro-electrodes in the excised and opened eye of the common frog, Rana temporaria. 2. When a single unit was isolated, the cell type was first determined according to the Maturana, Lettvin, McCulloch & Pitts (1960) classification with the aid of varying moving and stationary stimuli. 3. Class 4 cells respond only to a decrease of light when cones are stimulated but respond to an increase of light when green rods are stimulated. A distinct class of deviating class 4 cells was found that give a brief high frequency burst at 'off' from their small excitatory receptive fields (ERF); unlike typical class 4 cells they possess a purely inhibitory surrounding field (IRF).4. The contributions from the cones and the green and red rods were isolated by measuring the thresholds of the discharges with on-off stimuli of varying wave-lengths against strong yellow backgrounds, or against a very weak background or no background at all. The spatial distribution of the contributions to the ERF was determined by mapping threshold profiles, and additional information about ERF and IRF was obtained from area-threshold curves. 5. The cone-mediated ERFs were found to be 0-06-0-50 mm wide (1-5-12 degrees of visual field), which agrees well with the sizes of the dendritic trees of the ganglion cells. The green rod-mediated ERFs can be 0-5-1-5 mm wide and have less distinct boundaries than the cone-mediated. The green rod-mediated ERF of an individual ganglion cell is always larger than the cone-mediated ERF of the same cell. The red rod-mediated ERFs seem to be somewhat larger than the cone-mediated but smaller than the green rod-mediated. 6. The green rods contribute only to the on thresholds of class 1, 2 and 4 cells, but both to on and off in typical class 3 cells, while the cones contribute to on and off in classes 1-3 and only to off in class 4.7. When the red rods begin to contribute during dark adaptation they seem to enter the cone but not the green rod channels. 8. All three receptor types contribute to the IRF surrounding the ERF of classes 1, 2, 3 and deviating class 4 cells. Normal class 4 cells have no IRF. 9. The organization of the receptive fields is discussed in relation to the anatomy and electrophysiology of the cell types transmitting the signals from the receptors to the ganglion cells.", "contents": "Receptive field organization of ganglion cells in the frog retina: contributions from cones, green rods and red rods. 1. The impulse discharge of ganglion cells was recorded with extracellular micro-electrodes in the excised and opened eye of the common frog, Rana temporaria. 2. When a single unit was isolated, the cell type was first determined according to the Maturana, Lettvin, McCulloch & Pitts (1960) classification with the aid of varying moving and stationary stimuli. 3. Class 4 cells respond only to a decrease of light when cones are stimulated but respond to an increase of light when green rods are stimulated. A distinct class of deviating class 4 cells was found that give a brief high frequency burst at 'off' from their small excitatory receptive fields (ERF); unlike typical class 4 cells they possess a purely inhibitory surrounding field (IRF).4. The contributions from the cones and the green and red rods were isolated by measuring the thresholds of the discharges with on-off stimuli of varying wave-lengths against strong yellow backgrounds, or against a very weak background or no background at all. The spatial distribution of the contributions to the ERF was determined by mapping threshold profiles, and additional information about ERF and IRF was obtained from area-threshold curves. 5. The cone-mediated ERFs were found to be 0-06-0-50 mm wide (1-5-12 degrees of visual field), which agrees well with the sizes of the dendritic trees of the ganglion cells. The green rod-mediated ERFs can be 0-5-1-5 mm wide and have less distinct boundaries than the cone-mediated. The green rod-mediated ERF of an individual ganglion cell is always larger than the cone-mediated ERF of the same cell. The red rod-mediated ERFs seem to be somewhat larger than the cone-mediated but smaller than the green rod-mediated. 6. The green rods contribute only to the on thresholds of class 1, 2 and 4 cells, but both to on and off in typical class 3 cells, while the cones contribute to on and off in classes 1-3 and only to off in class 4.7. When the red rods begin to contribute during dark adaptation they seem to enter the cone but not the green rod channels. 8. All three receptor types contribute to the IRF surrounding the ERF of classes 1, 2, 3 and deviating class 4 cells. Normal class 4 cells have no IRF. 9. The organization of the receptive fields is discussed in relation to the anatomy and electrophysiology of the cell types transmitting the signals from the receptors to the ganglion cells."}
{"id": "PMID:1079536", "title": "Fluorescence intensity changes associated with contractile activation in frog muscle stained with Nile Blue A.", "content": "1. Extrinsic fluorescence intensity changes were studied in frog semitendinosus muscles stained with Nile Blue A in response to electrical stimulation. Muscles were stretched and put into hypertonic solutions to prevent movement. The muscles were illuminated at 90 degrees to their long axis with a narrow beam of light at a central wave-length of 6250 . Fluorescence emission was measured at 90 degrees to the exciting light using a filter which absorbed light of wave-lengths shorter than 6400 . 2. In response to a single stimulus the fluorescence intensity increases briefly. The fluorescence response is propagated at a constant velocity of about 1.5 m/sec. The average ratio of the maximum fluorescence intensity change to the resting fluorescence is 4.5 times 10-3 for supramaximal shocks. The fluorescence intensity change starts early in the falling phase of the action potential. 3. The fluorescence intensity change increases when nitrate replaces chloride and decreases when D2O replaces H2O. The rates of rise and fall of the fluorescence change was unaffected by nitrate replacement of chloride but are slowed where D2O replaces H2O. The rates of rise and fall of the fluorescence change increase with increasing temperature for all solutions used. The peak fluorescence intensity change, however, goes through a maximum at about 17 degrees C for aqueous chloride and nitrate solutions in the range of 10-25 degrees C. With D2O solutions, the peak fluorescence intensity increases monotonically in this range of temperatures. 4. The fluorescence intensity change in response to trains of action potentials are not additive. 5. Depolarization of muscles treated with tetrodotoxin using triangular-shaped fluid electrodes produces an increase in fluorescence at about the same threshold values required to elicit tension in preparations that are not fully stretched. The fluorescence intensity change precedes in time tension development. Near threshold depolarizations, the delay in onset of the fluorescence response can be 80 msec or longer. Byond threshold, delays become shorter and peak responses larger. During maintained depolarization, after the peak response, fluorescence declines to a plateau value. 6. The results suggest that the fluorescence intensity changes are associated with excitation-contraction coupling, possibly with changes in the transmembrane potential of the sarcoplasmic reticulum.", "contents": "Fluorescence intensity changes associated with contractile activation in frog muscle stained with Nile Blue A. 1. Extrinsic fluorescence intensity changes were studied in frog semitendinosus muscles stained with Nile Blue A in response to electrical stimulation. Muscles were stretched and put into hypertonic solutions to prevent movement. The muscles were illuminated at 90 degrees to their long axis with a narrow beam of light at a central wave-length of 6250 . Fluorescence emission was measured at 90 degrees to the exciting light using a filter which absorbed light of wave-lengths shorter than 6400 . 2. In response to a single stimulus the fluorescence intensity increases briefly. The fluorescence response is propagated at a constant velocity of about 1.5 m/sec. The average ratio of the maximum fluorescence intensity change to the resting fluorescence is 4.5 times 10-3 for supramaximal shocks. The fluorescence intensity change starts early in the falling phase of the action potential. 3. The fluorescence intensity change increases when nitrate replaces chloride and decreases when D2O replaces H2O. The rates of rise and fall of the fluorescence change was unaffected by nitrate replacement of chloride but are slowed where D2O replaces H2O. The rates of rise and fall of the fluorescence change increase with increasing temperature for all solutions used. The peak fluorescence intensity change, however, goes through a maximum at about 17 degrees C for aqueous chloride and nitrate solutions in the range of 10-25 degrees C. With D2O solutions, the peak fluorescence intensity increases monotonically in this range of temperatures. 4. The fluorescence intensity change in response to trains of action potentials are not additive. 5. Depolarization of muscles treated with tetrodotoxin using triangular-shaped fluid electrodes produces an increase in fluorescence at about the same threshold values required to elicit tension in preparations that are not fully stretched. The fluorescence intensity change precedes in time tension development. Near threshold depolarizations, the delay in onset of the fluorescence response can be 80 msec or longer. Byond threshold, delays become shorter and peak responses larger. During maintained depolarization, after the peak response, fluorescence declines to a plateau value. 6. The results suggest that the fluorescence intensity changes are associated with excitation-contraction coupling, possibly with changes in the transmembrane potential of the sarcoplasmic reticulum."}
{"id": "PMID:1079537", "title": "Energy balance in DNFB-treated and untreated frog muscle.", "content": "1. Heat production and chemical changes were measured in untreated and dinitrofluorobenzene (DNFB)-treated muscles during isometric tetani. Levels of total creatine (Ct), free creatine, ATP, ADP, AMP, inorganic phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, pyruvate, phosphoenolpyruvate, and lactate were measured. Changes in inosinic acid (IMP) were also measured. 2. DNFB effectively inhibited the creatine kinase reaction (Lohmann reaction). 3. Our major finding is that even after effective treatment with DNFB the observed heat plus work after 2 sec and 5 sec of stimulation is significantly greater than the enthalpy change produced by the measured chemical changes. This confirms that an unidentified exothermic process occurs during muscle contraction; this conclusion was reached previously from studies of untreated muscle. 4. The unexplained heat plus work is unlikely to be derived from glycolytic reactions since under anaerobic conditions no formation of lactate, pyruvate, phosphoenolpyruvate or fructose-1,6-diphosphate could be detected in either untreated or DNFB-treated muscles even 34 sec after a series of three 5 sec isometric tetani. 5. In the first 2 sec of stimulation the unexplained heat plus work is less in DNFB-treated muscles than in untreated muscles. However from 2 to 5 sec of stimulation the unexplained heat plus work is the same in treated and untreated muscles.", "contents": "Energy balance in DNFB-treated and untreated frog muscle. 1. Heat production and chemical changes were measured in untreated and dinitrofluorobenzene (DNFB)-treated muscles during isometric tetani. Levels of total creatine (Ct), free creatine, ATP, ADP, AMP, inorganic phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, pyruvate, phosphoenolpyruvate, and lactate were measured. Changes in inosinic acid (IMP) were also measured. 2. DNFB effectively inhibited the creatine kinase reaction (Lohmann reaction). 3. Our major finding is that even after effective treatment with DNFB the observed heat plus work after 2 sec and 5 sec of stimulation is significantly greater than the enthalpy change produced by the measured chemical changes. This confirms that an unidentified exothermic process occurs during muscle contraction; this conclusion was reached previously from studies of untreated muscle. 4. The unexplained heat plus work is unlikely to be derived from glycolytic reactions since under anaerobic conditions no formation of lactate, pyruvate, phosphoenolpyruvate or fructose-1,6-diphosphate could be detected in either untreated or DNFB-treated muscles even 34 sec after a series of three 5 sec isometric tetani. 5. In the first 2 sec of stimulation the unexplained heat plus work is less in DNFB-treated muscles than in untreated muscles. However from 2 to 5 sec of stimulation the unexplained heat plus work is the same in treated and untreated muscles."}
{"id": "PMID:1079538", "title": "The effects of prolonged repetitive stimulation in hemicholinium on the frog neuromuscular junction.", "content": "1. Cutaneous pectoris nerve-muscle preparations from the frog were stimulated for prolonged periods in solutions with curare alone, curare and hemicholinium no. 3 (HC-3), or curare and glucose plus choline. End-plate potentials (e.p.p.s) and miniature end-plate potentials (m.e.p.p.s) were recorded intracellularly. Black widow spider venom (BWSV) was applied to determine the degree of depletion of the transmitter stores. 2. The ultrastructure of the neuromuscular junctions was studied in the electron microscope. Some of the preparations were fixed immediately at the end of the period of stimulation and others were fixed about an hour after BWSV had been applied. In some experiments horseradish peroxidase (HRP) was present during the period of stimulation and the fixed tissue was treated to reveal the distribution of the tracer. 3. The amplitude of the e.p.p. fell rapidly to almost zero during 2 hr of stimulation at 2/sec in 100 muM HC-3 and little recovery occurred during a subsequent hour of rest. About 2-7 times 10-5 quanta were secreted. The e.p.p.s usually persisted throughout the period of stimulation in the other solutions and 2-2-6 times as much transmitter was secreted. 4. When BWSV was applied immediately at the end of the period of stimulation in HC-3, almost no m.e.p.p.s were discharged and only small m.e.p.p.s were discharged when the venom was applied after an hour of rest. 5. When BWSV was applied to unstimulated terminals that had been bathed in HC-3, or to terminals that had been stimulated and rested for an hour in glucose plus choline, m.e.p.p.s of nearly normal amplitude were discharged. 6. Terminals stimulated for 2 hr at 2/sec in 100 muM HC-3 contained a normal complement of synaptic vesicles and a large proportion of vesicles were labelled with HRP when the tracer was present during the period of stimulation. 7. BWSV induced the almost complete depletion of vesicles from terminals that had been stimulated in HC-3. 8. Depletion of vesicles also occurred when terminals were stimulated for 20 min at 10/sec after they had been previously stimulated for 2 hr at 2/sec in HC-3. These terminals showed extensive infolding of the axolemma and they contained swollen mitochondria. 9. These results indicate that stimulation in HC-3 depletes terminals of their store of transmitter but not of their population of vesicles and that vesicles empty of transmitter can fuse with and reform from the axolemma of the nerve terminal.", "contents": "The effects of prolonged repetitive stimulation in hemicholinium on the frog neuromuscular junction. 1. Cutaneous pectoris nerve-muscle preparations from the frog were stimulated for prolonged periods in solutions with curare alone, curare and hemicholinium no. 3 (HC-3), or curare and glucose plus choline. End-plate potentials (e.p.p.s) and miniature end-plate potentials (m.e.p.p.s) were recorded intracellularly. Black widow spider venom (BWSV) was applied to determine the degree of depletion of the transmitter stores. 2. The ultrastructure of the neuromuscular junctions was studied in the electron microscope. Some of the preparations were fixed immediately at the end of the period of stimulation and others were fixed about an hour after BWSV had been applied. In some experiments horseradish peroxidase (HRP) was present during the period of stimulation and the fixed tissue was treated to reveal the distribution of the tracer. 3. The amplitude of the e.p.p. fell rapidly to almost zero during 2 hr of stimulation at 2/sec in 100 muM HC-3 and little recovery occurred during a subsequent hour of rest. About 2-7 times 10-5 quanta were secreted. The e.p.p.s usually persisted throughout the period of stimulation in the other solutions and 2-2-6 times as much transmitter was secreted. 4. When BWSV was applied immediately at the end of the period of stimulation in HC-3, almost no m.e.p.p.s were discharged and only small m.e.p.p.s were discharged when the venom was applied after an hour of rest. 5. When BWSV was applied to unstimulated terminals that had been bathed in HC-3, or to terminals that had been stimulated and rested for an hour in glucose plus choline, m.e.p.p.s of nearly normal amplitude were discharged. 6. Terminals stimulated for 2 hr at 2/sec in 100 muM HC-3 contained a normal complement of synaptic vesicles and a large proportion of vesicles were labelled with HRP when the tracer was present during the period of stimulation. 7. BWSV induced the almost complete depletion of vesicles from terminals that had been stimulated in HC-3. 8. Depletion of vesicles also occurred when terminals were stimulated for 20 min at 10/sec after they had been previously stimulated for 2 hr at 2/sec in HC-3. These terminals showed extensive infolding of the axolemma and they contained swollen mitochondria. 9. These results indicate that stimulation in HC-3 depletes terminals of their store of transmitter but not of their population of vesicles and that vesicles empty of transmitter can fuse with and reform from the axolemma of the nerve terminal."}
{"id": "PMID:1079541", "title": "B and T cells in a cat with thymic lymphosarcoma.", "content": "The mixed antiglobulin reaction and the formation of nonimmune rosettes with guinea-pig red blood cells (RBC) distinguished feline B and T cells, respectively. In a cat with thymic lymphosarcoma, the cells reacting in these tests formed separate, nonoverlapping populations. The malignant cells were large lymphoblasts replacing the normal thymus and infiltrating local lymph nodes, where they localized only in the paracortical, i.e., thymus-dependent areas. Cells from the nodes could therefore be identified as malignant or normal by their size. The mixed antiglobulin reaction showed that the malignant cells did not carry the surface Ig characteristic of B cells, whereas these malignant cells formed nonimmune rosettes with guinea-pig RBC. Among lymph node cells, most surviving normal and small lymphocytes, from outside the thymus-dependent areas, reacted as B  cells. The morphologic evidence therefore corroborated the test results, which indicated that the formation of rosettes with guinea-pig RBC seems a reliable means for the demonstration of T cells in the cat.", "contents": "B and T cells in a cat with thymic lymphosarcoma. The mixed antiglobulin reaction and the formation of nonimmune rosettes with guinea-pig red blood cells (RBC) distinguished feline B and T cells, respectively. In a cat with thymic lymphosarcoma, the cells reacting in these tests formed separate, nonoverlapping populations. The malignant cells were large lymphoblasts replacing the normal thymus and infiltrating local lymph nodes, where they localized only in the paracortical, i.e., thymus-dependent areas. Cells from the nodes could therefore be identified as malignant or normal by their size. The mixed antiglobulin reaction showed that the malignant cells did not carry the surface Ig characteristic of B cells, whereas these malignant cells formed nonimmune rosettes with guinea-pig RBC. Among lymph node cells, most surviving normal and small lymphocytes, from outside the thymus-dependent areas, reacted as B  cells. The morphologic evidence therefore corroborated the test results, which indicated that the formation of rosettes with guinea-pig RBC seems a reliable means for the demonstration of T cells in the cat."}
{"id": "PMID:1079550", "title": "Aorta-coronary artery saphenous vein bypass grafts. Isolated and combined with other procedures.", "content": "The results in 578 patients who underwent a saphenous vein bypass graft operation (isolated SVBG) or a SVBG combined with other procedures (combined SVBG) from 1969 through 1972 were analyzed. The operative mortality rate was low (3 percent) for isolated SVBG but increased considerably for combined SVBG. The late mortality rate was also low (4 percent) for isolated SVBG and also increased markedly for combined SVBG. The operative mortality rate did not change in the group undergoing left ventricular aneurysmectomy or mitral valve surgery for postinfarction mitral insufficiency, whether or not SVBG was used; however, late results were better in both groups when SVBG was performed. Among all groups, patients with postinfarction mitral insufficiency or rheumatic mitral incompetence associated with coronary artery disease had the poorest outcome. Despite the operative and late deaths, patients undergoing SVBG with aortic valve replacement showed excellent results in the group of survivors.", "contents": "Aorta-coronary artery saphenous vein bypass grafts. Isolated and combined with other procedures. The results in 578 patients who underwent a saphenous vein bypass graft operation (isolated SVBG) or a SVBG combined with other procedures (combined SVBG) from 1969 through 1972 were analyzed. The operative mortality rate was low (3 percent) for isolated SVBG but increased considerably for combined SVBG. The late mortality rate was also low (4 percent) for isolated SVBG and also increased markedly for combined SVBG. The operative mortality rate did not change in the group undergoing left ventricular aneurysmectomy or mitral valve surgery for postinfarction mitral insufficiency, whether or not SVBG was used; however, late results were better in both groups when SVBG was performed. Among all groups, patients with postinfarction mitral insufficiency or rheumatic mitral incompetence associated with coronary artery disease had the poorest outcome. Despite the operative and late deaths, patients undergoing SVBG with aortic valve replacement showed excellent results in the group of survivors."}
{"id": "PMID:1079551", "title": "Serum enzyme data in diagnosis of myocardial infarction during or early after aorta-coronary saphenous vein bypass graft operations.", "content": "In 397 cases, serum glutamic oxaloacetic transaminase (GOT) and creatine phosphokinase (CPK) values were determined on the first, second, and third days after aorta-coronary artery saphenous vein bypass graft operations. Electrocardiographic (ECG) or vectorcardiographic (VCG) evidence of postoperative transmural infarction was found in 48 cases. Multivariate analysis indicated that GOT and CPK concentrations on day 1 had substantial discriminatory value regarding infarction. Discrimination by GOT values was not significantly improved by addition of CPK. Of the 61 cases positive by GOT values measured 1 day postoperatively (greater than 100 U. per liter; normal smaller than 24), 32 (52 percent) were negative by ECG or VCG; of the 336 cases negative by GOT, 19 (6 percent) were positive by ECG or VCG. GOT and CPK values increased with the number of vessels grafted and with the use of ventricular rather than atrial vents. Total serum enzyme values lack specificty in diagnosis of transmural infarction after the saphenous vein bypass operation.", "contents": "Serum enzyme data in diagnosis of myocardial infarction during or early after aorta-coronary saphenous vein bypass graft operations. In 397 cases, serum glutamic oxaloacetic transaminase (GOT) and creatine phosphokinase (CPK) values were determined on the first, second, and third days after aorta-coronary artery saphenous vein bypass graft operations. Electrocardiographic (ECG) or vectorcardiographic (VCG) evidence of postoperative transmural infarction was found in 48 cases. Multivariate analysis indicated that GOT and CPK concentrations on day 1 had substantial discriminatory value regarding infarction. Discrimination by GOT values was not significantly improved by addition of CPK. Of the 61 cases positive by GOT values measured 1 day postoperatively (greater than 100 U. per liter; normal smaller than 24), 32 (52 percent) were negative by ECG or VCG; of the 336 cases negative by GOT, 19 (6 percent) were positive by ECG or VCG. GOT and CPK values increased with the number of vessels grafted and with the use of ventricular rather than atrial vents. Total serum enzyme values lack specificty in diagnosis of transmural infarction after the saphenous vein bypass operation."}
{"id": "PMID:1079552", "title": "Delayed coronary artery dissection after angiography. A case description with successful operative treatment.", "content": "A patient is described in whom right coronary artery dissection occurred 48 hours after coronary angiography. Successful operative treatment consisted of immediate saphenous vein bypass grafting and ligation of the proximal coronary artery. Several aspects of the case are characteristic of this pathological entity as reported in the literature and suggest principles of surgical management.", "contents": "Delayed coronary artery dissection after angiography. A case description with successful operative treatment. A patient is described in whom right coronary artery dissection occurred 48 hours after coronary angiography. Successful operative treatment consisted of immediate saphenous vein bypass grafting and ligation of the proximal coronary artery. Several aspects of the case are characteristic of this pathological entity as reported in the literature and suggest principles of surgical management."}
{"id": "PMID:1079581", "title": "A study of B and T cells in multiple sclerosis.", "content": "Peripheral blood T and B lymphocytes were enumerated using E and EAC red blood cell rosetting techniques. The percentage of EAC-binding cells in peripheral blood of multiple sclerosis patients is elevated (31.7 plus or minus 2.4S.E.M.) when compared with the percentage in healthy controls (18.5 plus or minus 1.1 S.E.M.). The total T cell percentage is not significantly lower in multiple sclerosis than in controls, but fewer T cells in patients with multiple sclerosis are able to bind 10 or more red blood cells.", "contents": "A study of B and T cells in multiple sclerosis. Peripheral blood T and B lymphocytes were enumerated using E and EAC red blood cell rosetting techniques. The percentage of EAC-binding cells in peripheral blood of multiple sclerosis patients is elevated (31.7 plus or minus 2.4S.E.M.) when compared with the percentage in healthy controls (18.5 plus or minus 1.1 S.E.M.). The total T cell percentage is not significantly lower in multiple sclerosis than in controls, but fewer T cells in patients with multiple sclerosis are able to bind 10 or more red blood cells."}
{"id": "PMID:1079587", "title": "In vitro resistance of haemophilus influenzae to co-trimoxazole and trimethoprim.", "content": "Over a period of five months Haemophilus influenzae isolated from 123 patients were tested for resistance to co-trimoxazole and trimethoprim by the disc method of sensitivity testing and isolates from four patients were found to be resistant. However, the minimum inhibitory concentrations of trimethoprim lactate were estimated for the isolates from these four patients only one was found to be resistant. This H. influenzae strain was from one of the two patients in the survey who had a history of repeated courses of co-trimoxazole therapy. The discrepancy between the results of the disc sensitivity tests and the minimum inhibitory concentration estimations of the resistance of H. influenzae to co-trimoxazole and trimethoprim. The appearance of co-trimoxazole and trimethoprim resistance in H. influenzae isolated from a patient who has had prolonged treatment with co-trimoxazole is in agreement with the findings of other authors.", "contents": "In vitro resistance of haemophilus influenzae to co-trimoxazole and trimethoprim. Over a period of five months Haemophilus influenzae isolated from 123 patients were tested for resistance to co-trimoxazole and trimethoprim by the disc method of sensitivity testing and isolates from four patients were found to be resistant. However, the minimum inhibitory concentrations of trimethoprim lactate were estimated for the isolates from these four patients only one was found to be resistant. This H. influenzae strain was from one of the two patients in the survey who had a history of repeated courses of co-trimoxazole therapy. The discrepancy between the results of the disc sensitivity tests and the minimum inhibitory concentration estimations of the resistance of H. influenzae to co-trimoxazole and trimethoprim. The appearance of co-trimoxazole and trimethoprim resistance in H. influenzae isolated from a patient who has had prolonged treatment with co-trimoxazole is in agreement with the findings of other authors."}
{"id": "PMID:1079590", "title": "Exogenous Moraxella liquefaciens endophthalmitis.", "content": "Moraxella species have been generally associated with diseases of the external eye. In this report we present a case of exogenous Moraxella liquefaciens endothalmitis which developed 14 years after an intracapsular cataract extraction.", "contents": "Exogenous Moraxella liquefaciens endophthalmitis. Moraxella species have been generally associated with diseases of the external eye. In this report we present a case of exogenous Moraxella liquefaciens endothalmitis which developed 14 years after an intracapsular cataract extraction."}
{"id": "PMID:1079595", "title": "Acupuncture and transdermal electrostimulation in the treatment of deafness.", "content": "A review and critique of the research on acupuncture and transdermal electrostimulation therapy in treating sensorineural hearing loss is presented. Of 111 subjects in these studies were noted to exhibit a decrease in hearing sensitivity. Additional data collected at our clinics on four preschool hearing-impaired children failed to provide evidence of improvement following acupuncture. Similar results are reported with transdermal therapy, suggesting that both procedures are inappropriate for the treatment of sensorineural hearing loss.", "contents": "Acupuncture and transdermal electrostimulation in the treatment of deafness. A review and critique of the research on acupuncture and transdermal electrostimulation therapy in treating sensorineural hearing loss is presented. Of 111 subjects in these studies were noted to exhibit a decrease in hearing sensitivity. Additional data collected at our clinics on four preschool hearing-impaired children failed to provide evidence of improvement following acupuncture. Similar results are reported with transdermal therapy, suggesting that both procedures are inappropriate for the treatment of sensorineural hearing loss."}
{"id": "PMID:1079597", "title": "Displacement currents in the node of Ranvier. Voltage and time dependence.", "content": "The time course of the membrane currents in the node of Ranvier in which the sodium and potassium conductances have been blocked reveals asymmetries during and after the application of depolarizing and hyperpolarizing voltage-clamp pulses of identical size. Since 1. the integrals of the \"on\" and \"off\" current transients were found to be equal and opposite, 2. the change displaced reached saturation (about 140-10-minus 15 C/node) when the internal potential was taken to a sufficiently positive value during the depolarizing pulses and, 3. the size of the charge transferred was unaffected by temperature although its time constant had a large temperature coefficient (Q10 = 2.4), these currents to our opinion must result form charge movements confined to the membrane and, therefore, can be considered as non-liner displacement currents. The steady-state rearrangement of the charges is consistent with a Boltzmann distribution of charges (effective valence z = 1.65) between two configurations characterized by different energy levels. The midpoint potential of the distribution curve is -33.7mV and its maximum slope, kT/ZE, is 14.9 mV. Following changes in membrane potential the charges undergo a first order transition between these states. We propose that these displacement currents arise from a redistribution of the charges involved in the sodium gating system.", "contents": "Displacement currents in the node of Ranvier. Voltage and time dependence. The time course of the membrane currents in the node of Ranvier in which the sodium and potassium conductances have been blocked reveals asymmetries during and after the application of depolarizing and hyperpolarizing voltage-clamp pulses of identical size. Since 1. the integrals of the \"on\" and \"off\" current transients were found to be equal and opposite, 2. the change displaced reached saturation (about 140-10-minus 15 C/node) when the internal potential was taken to a sufficiently positive value during the depolarizing pulses and, 3. the size of the charge transferred was unaffected by temperature although its time constant had a large temperature coefficient (Q10 = 2.4), these currents to our opinion must result form charge movements confined to the membrane and, therefore, can be considered as non-liner displacement currents. The steady-state rearrangement of the charges is consistent with a Boltzmann distribution of charges (effective valence z = 1.65) between two configurations characterized by different energy levels. The midpoint potential of the distribution curve is -33.7mV and its maximum slope, kT/ZE, is 14.9 mV. Following changes in membrane potential the charges undergo a first order transition between these states. We propose that these displacement currents arise from a redistribution of the charges involved in the sodium gating system."}
{"id": "PMID:1079601", "title": "Voltage-dependence of drug-induced conductance in frog neuromuscular junction.", "content": "Membrane currents from voltage-clamped frog muscle fibers were recorded during iontophoretic application of steady doses of carbachol, acetylcholine, and suberyldicholine to the endplate region. In the presence of these drugs, an exponentially relaxing current was observed after step changes of membrane potential. The time constant of relaxation was found to be voltage-dependent. It was equal to the time constant obtained from the autocorrelation function of drug-induced conductance fluctuations measured under similar conditions. Analysis of instantaneous current at the on- and offsets of voltageclamp pulses showed that there is no shift in equilibrium potential during the pulses.", "contents": "Voltage-dependence of drug-induced conductance in frog neuromuscular junction. Membrane currents from voltage-clamped frog muscle fibers were recorded during iontophoretic application of steady doses of carbachol, acetylcholine, and suberyldicholine to the endplate region. In the presence of these drugs, an exponentially relaxing current was observed after step changes of membrane potential. The time constant of relaxation was found to be voltage-dependent. It was equal to the time constant obtained from the autocorrelation function of drug-induced conductance fluctuations measured under similar conditions. Analysis of instantaneous current at the on- and offsets of voltageclamp pulses showed that there is no shift in equilibrium potential during the pulses."}
{"id": "PMID:1079602", "title": "Picosecond detection of an intermediate in the photochemical reaction of bacterial photosynthesis.", "content": "Preparations of photosynthetic reaction centers from Rhodopseudomonas sphaeroides were excited with flashes lasting approximately 8 psec. Immediately after the excitation, there appeared a transient state which was characterized by new absorption bands near 500 and 680 nm, by a bleaching of bands near 540, 600, 760, and 870 nm, and by a blue shift of a band near 800 nm. The transient state decayed with an exponential decay time,t, of 246 plus or minus 16 psec after the flash. As the transient state decayed, the radical cation of the reaction center bacteriochlorophyll complex appeared. This indicates that the transient state is an intermediate in the photooxidation of the bacteriochlorophyll. The absorpiton spectrum of the transient state shows the state to be identical with a state (P-F) which has been detected previously in reaction centers that are prevented from completing the photooxidation, because of chemical reduction of the electron acceptor. Analysis of the spectrum suggests that the formation of P-F involves electron transfer from one bacteriochlorophyll molecule to another within the reaction center, or possibly from bacteriochlorophyll to the bacteriopheophytin of the complex. The initial absorbance changes after flash excitation also include a bleaching of an absorption band at 800 nm. The bleaching decays with tau approximately equal to 30 pse. The bleaching appers not to be a secondary effect, but rather to revael another early step in the primary photochemical reaction.", "contents": "Picosecond detection of an intermediate in the photochemical reaction of bacterial photosynthesis. Preparations of photosynthetic reaction centers from Rhodopseudomonas sphaeroides were excited with flashes lasting approximately 8 psec. Immediately after the excitation, there appeared a transient state which was characterized by new absorption bands near 500 and 680 nm, by a bleaching of bands near 540, 600, 760, and 870 nm, and by a blue shift of a band near 800 nm. The transient state decayed with an exponential decay time,t, of 246 plus or minus 16 psec after the flash. As the transient state decayed, the radical cation of the reaction center bacteriochlorophyll complex appeared. This indicates that the transient state is an intermediate in the photooxidation of the bacteriochlorophyll. The absorpiton spectrum of the transient state shows the state to be identical with a state (P-F) which has been detected previously in reaction centers that are prevented from completing the photooxidation, because of chemical reduction of the electron acceptor. Analysis of the spectrum suggests that the formation of P-F involves electron transfer from one bacteriochlorophyll molecule to another within the reaction center, or possibly from bacteriochlorophyll to the bacteriopheophytin of the complex. The initial absorbance changes after flash excitation also include a bleaching of an absorption band at 800 nm. The bleaching decays with tau approximately equal to 30 pse. The bleaching appers not to be a secondary effect, but rather to revael another early step in the primary photochemical reaction."}
{"id": "PMID:1079605", "title": "Aneurysm of the gastroduodenal artery.", "content": "Report on the case of a woman having a history of duodenal ulcer, who underwent six unsuccessful operations for several hemorrhages of the digestive tract. When angiography was finally carried out, this revealed an aneurysm of the gastroduodenal artery, probably located at the base of the ulcer. The diagnosis was verified at the subsequent operation which made it possible to arrest the haemorrhage by ligating the aneurysm.", "contents": "Aneurysm of the gastroduodenal artery. Report on the case of a woman having a history of duodenal ulcer, who underwent six unsuccessful operations for several hemorrhages of the digestive tract. When angiography was finally carried out, this revealed an aneurysm of the gastroduodenal artery, probably located at the base of the ulcer. The diagnosis was verified at the subsequent operation which made it possible to arrest the haemorrhage by ligating the aneurysm."}
{"id": "PMID:1079604", "title": "Age at maternal separation and gastric erosion susceptibility in the rat.", "content": "The development of susceptibility to immobilization-induced gastric erosions was studied in laboratory rats previously separated from their mothers at 15, 21 or 25 days of age. Early separation (day 15) produced animals whose maximum susceptibility occurred at a much younger age, generated a susceptibility curve over life that was the inverse of the curve for animals separated later and led to severe gastrointestinal hemorrhage as a common and distinguishing complication in younger rats. The pathogenesis of erosion formation in early separated rats may be unique in that, for that group only, the food deprivation component of the immobilization paradigm, when presented alone, also produced erosion of the glandular stomach, with hemorrhage.", "contents": "Age at maternal separation and gastric erosion susceptibility in the rat. The development of susceptibility to immobilization-induced gastric erosions was studied in laboratory rats previously separated from their mothers at 15, 21 or 25 days of age. Early separation (day 15) produced animals whose maximum susceptibility occurred at a much younger age, generated a susceptibility curve over life that was the inverse of the curve for animals separated later and led to severe gastrointestinal hemorrhage as a common and distinguishing complication in younger rats. The pathogenesis of erosion formation in early separated rats may be unique in that, for that group only, the food deprivation component of the immobilization paradigm, when presented alone, also produced erosion of the glandular stomach, with hemorrhage."}
{"id": "PMID:1079608", "title": "Comparison of ventricular enlargement and radiopharmaceutical retention: a cisternographic-pneumoencephalographic comparison.", "content": "The cisternograms and pneumoencephalograms of 58 patients with suspected \"normal pressure\" hydrocephalus were correlated and the relationship of ventricular radiopharmaceutical entry and stasis to ventricular size was analyzed. It was found that radiopharmaceutical entry relates directly to ventricular size, stasis occurring only in markedly enlarged ventricles. Cerebrospinal fluid imaging alone is a highly reliable diagnostic study only if patients exhibit the characteristic cisternographic patterns of normal pressure hydrocephalus. In those who do not, pneumoencephalography and cisternography are valuable complementary studies which can also be used to identify primary cortical atrophy.", "contents": "Comparison of ventricular enlargement and radiopharmaceutical retention: a cisternographic-pneumoencephalographic comparison. The cisternograms and pneumoencephalograms of 58 patients with suspected \"normal pressure\" hydrocephalus were correlated and the relationship of ventricular radiopharmaceutical entry and stasis to ventricular size was analyzed. It was found that radiopharmaceutical entry relates directly to ventricular size, stasis occurring only in markedly enlarged ventricles. Cerebrospinal fluid imaging alone is a highly reliable diagnostic study only if patients exhibit the characteristic cisternographic patterns of normal pressure hydrocephalus. In those who do not, pneumoencephalography and cisternography are valuable complementary studies which can also be used to identify primary cortical atrophy."}
{"id": "PMID:1079609", "title": "The efficacy of the brain scan in diagnosis of brainstem gliomas.", "content": "Nineteen cases of brainstem glioma were reviewed to evaluate lesion detection by brain imaging and other radiological methods. 99m-Tc-pertechnetate brain imaging was definitely abnormal in only one patient. Other techniques proved to be more sensitive and specific. Angiograms were abnormal in 10 of 18 cases while pneumoencephalography demonstrated the lesion in 16 of 19 cases. Brain imaging is not a sensitive diagnostic study in patients with suspected brainstem glioma. A normal brain scan is not useful in excluding this diagnosis and further radiological evaluation is necessary.", "contents": "The efficacy of the brain scan in diagnosis of brainstem gliomas. Nineteen cases of brainstem glioma were reviewed to evaluate lesion detection by brain imaging and other radiological methods. 99m-Tc-pertechnetate brain imaging was definitely abnormal in only one patient. Other techniques proved to be more sensitive and specific. Angiograms were abnormal in 10 of 18 cases while pneumoencephalography demonstrated the lesion in 16 of 19 cases. Brain imaging is not a sensitive diagnostic study in patients with suspected brainstem glioma. A normal brain scan is not useful in excluding this diagnosis and further radiological evaluation is necessary."}
{"id": "PMID:1079610", "title": "The validity of criteria for the evaluation of cerebral atrophy by computed tomography.", "content": "Computed tomography of the brain was used in evaluating demented and nondemented elderly people. The incidence of convulutional atrophy and ventricular enlargement correlated well with pathologic and pneumoencephalographic examination of a similar population. Computed tomography also showed certain instances of dementia without atrophy and evidence of atrophy without dementia. The absence of brain atrophy in a demented patient should prompt a search for a potentially treatable cause of the dementia. The authors discuss criteria for diagnosing atrophy by computed tomography and conclude that, because of its noninvasive nature, it is a valuable aid in screening demented patients for treatable diseases.", "contents": "The validity of criteria for the evaluation of cerebral atrophy by computed tomography. Computed tomography of the brain was used in evaluating demented and nondemented elderly people. The incidence of convulutional atrophy and ventricular enlargement correlated well with pathologic and pneumoencephalographic examination of a similar population. Computed tomography also showed certain instances of dementia without atrophy and evidence of atrophy without dementia. The absence of brain atrophy in a demented patient should prompt a search for a potentially treatable cause of the dementia. The authors discuss criteria for diagnosing atrophy by computed tomography and conclude that, because of its noninvasive nature, it is a valuable aid in screening demented patients for treatable diseases."}
{"id": "PMID:1079628", "title": "Concurrence of ulcerative colitis and chronic acitve hepatitis, Clinical courses and results of colectomy.", "content": "Seven patients with coexisting chronic active hepatitis and ulcerative colitis are reviewed. The activity of the two diseases varied independently. Proctocolectomy was necessitated in 6 patients and was followed by disappearance or decreased in titres of smooth muscle and antinuclear autoantibodies and immunoglobulins and, in one patient, by a rapid normalization of markedly elevated transaminases. Although the series are small, we feel that our data suggest that proctocolectomy for severe ulcerative colitis may have some beneficial effect on a coexisting chronic active hepatitis.", "contents": "Concurrence of ulcerative colitis and chronic acitve hepatitis, Clinical courses and results of colectomy. Seven patients with coexisting chronic active hepatitis and ulcerative colitis are reviewed. The activity of the two diseases varied independently. Proctocolectomy was necessitated in 6 patients and was followed by disappearance or decreased in titres of smooth muscle and antinuclear autoantibodies and immunoglobulins and, in one patient, by a rapid normalization of markedly elevated transaminases. Although the series are small, we feel that our data suggest that proctocolectomy for severe ulcerative colitis may have some beneficial effect on a coexisting chronic active hepatitis."}
{"id": "PMID:1079629", "title": "Structural requirements in the Fc region of rabbit IgG antibodies necessary to induce cytotoxicity by human lymphocytes.", "content": "Rabbit IgG anti-chicken erythrocyte antibodies were compared with the Fab/c or Facb fragments of IgG and with partially reduced and alkylated IgG for the capacity to induce cytotoxicity by normal human lymphocytes. The Fab/c antibody fragment, which lacks one Fab region, was still able to induce cytotoxicity. In contrast, the Facb antibody fragment, which lacks the C-gamma3 domains, was nearly ineffective in activating the effector cells, whereas intact antibody activity was demonstrated by its ability to inhibit the cytotoxicity induced by unsplit IgG. Similarly, partial reduction and alkylation of the IgG antibodies, under conditions affecting the interchain disulphide bonds only, greatly diminished their ability to induce cytotoxicity, although they effectively inhibited the cytotoxicity induced by untreated IgG. On the basis of these results and previous data, we suggest that the reaction of the Fc region of IgG with the effector cell depends on the integrity of the C-gamma2 domain in the native, divalent state or on the interaction between the C-gamma2 and C-gamma3 domains.", "contents": "Structural requirements in the Fc region of rabbit IgG antibodies necessary to induce cytotoxicity by human lymphocytes. Rabbit IgG anti-chicken erythrocyte antibodies were compared with the Fab/c or Facb fragments of IgG and with partially reduced and alkylated IgG for the capacity to induce cytotoxicity by normal human lymphocytes. The Fab/c antibody fragment, which lacks one Fab region, was still able to induce cytotoxicity. In contrast, the Facb antibody fragment, which lacks the C-gamma3 domains, was nearly ineffective in activating the effector cells, whereas intact antibody activity was demonstrated by its ability to inhibit the cytotoxicity induced by unsplit IgG. Similarly, partial reduction and alkylation of the IgG antibodies, under conditions affecting the interchain disulphide bonds only, greatly diminished their ability to induce cytotoxicity, although they effectively inhibited the cytotoxicity induced by untreated IgG. On the basis of these results and previous data, we suggest that the reaction of the Fc region of IgG with the effector cell depends on the integrity of the C-gamma2 domain in the native, divalent state or on the interaction between the C-gamma2 and C-gamma3 domains."}
{"id": "PMID:1079630", "title": "Teeth loss and the inflammation of teeth-supporting tissues in rheumatoid disease.", "content": "To compare the state of dentition between rheumatoid and control patients, ortopanthomograms were taken of 126 rheumatoid and 119 control patients. This roenthenologic examination revealed a signficantly higher incidence of teeth loss among the rheumatoid patients than among the control patients. To determine whether the dental periapical lesions of patients with rheumatoid disease differ histologically from corresponding lesions in control patients, the dental periapical lesions of 43 rheumatoid and 23 control patients were examined with a routine histochemical technique. No differences were detected however between these two patient groups either as regards the histologic features of their periapical lesions or as regards the incidence of these lesions, whether granulomas or radicular cysts. Furthermore, examination of the gingival tissues from 21 rheumatoid patients did not either reveal any distinguishing histologic feature. Amyloid was sought in the teeth-supporting tissues of both rheumatoid and control groups with techniques involving light, polarization and fluorescence microscopy. In neither group was amyloid detected with certainty with any of these techniques. The higher incidence of teeth loss among rheumatoid patients invited further investigation into the relationship between dental health and rheumatoid disease.", "contents": "Teeth loss and the inflammation of teeth-supporting tissues in rheumatoid disease. To compare the state of dentition between rheumatoid and control patients, ortopanthomograms were taken of 126 rheumatoid and 119 control patients. This roenthenologic examination revealed a signficantly higher incidence of teeth loss among the rheumatoid patients than among the control patients. To determine whether the dental periapical lesions of patients with rheumatoid disease differ histologically from corresponding lesions in control patients, the dental periapical lesions of 43 rheumatoid and 23 control patients were examined with a routine histochemical technique. No differences were detected however between these two patient groups either as regards the histologic features of their periapical lesions or as regards the incidence of these lesions, whether granulomas or radicular cysts. Furthermore, examination of the gingival tissues from 21 rheumatoid patients did not either reveal any distinguishing histologic feature. Amyloid was sought in the teeth-supporting tissues of both rheumatoid and control groups with techniques involving light, polarization and fluorescence microscopy. In neither group was amyloid detected with certainty with any of these techniques. The higher incidence of teeth loss among rheumatoid patients invited further investigation into the relationship between dental health and rheumatoid disease."}
{"id": "PMID:1079635", "title": "Treatment of vaginal discharge with yoghurt.", "content": "A preliminary trial was  conducted, using yoghurt to assess its potential for the management of vaginal discharge. The results were encouraging in non-specific vaginal discharge and warrant further investigation. The treatment was ineffective in reducing Trichomonas vaginalis infestation, and in this condition no further investigation is warranted.", "contents": "Treatment of vaginal discharge with yoghurt. A preliminary trial was  conducted, using yoghurt to assess its potential for the management of vaginal discharge. The results were encouraging in non-specific vaginal discharge and warrant further investigation. The treatment was ineffective in reducing Trichomonas vaginalis infestation, and in this condition no further investigation is warranted."}
{"id": "PMID:1079640", "title": "[Recurrent haemoptysis for a period of five years following aspiration of a gall stone].", "content": "The article deals with a case report of a 71-years-old female who suffered from hemoptysis over a period of five years. The first hemoptysis occurred postoperatively after a cholecystectomy in December 1966 and was thought to be caused by pulmonary embolism. Persisting episodes of more or less slight hemoptysis in the following years lead to repeated different diagnostic procedures. Radiologically there was an infiltration of the right lower lobe rather constant in size. Endoscopically no bronchial obstruction was visible. In July 1972 the patient suffered from a more severe hemoptoe. X-ray findings were nearly unchanged. With bronchoscopy the hemorrhage could be localized to the segmental bronchi 8 and 9. Right lower lobectomy was done in September 1972 without an exact preoperative diagnosis. The patient had an uneventful postoperative course despite of her age. Pathological examination of the specimen showed a foreign body looking like a gallstone. This diagnosis was confirmed by chemical analysis. To get an explanation we inquired into the circumstances of the cholecystectomy performed 5 years ago. These lead to the conclusion that the concrement must have been vomited and aspirated by the evidently not intubated patient. These suggestions were supported by the fact, that the former operation has been done because of a gangrenous gallbladder with a highly delated common duct and the duodenum, stomach and transverse colon being involved in an inflammatory infiltration. Dangers of asymptomic foreign body aspiration and diagnostic problems in non-opaque peripheral foreign bodies are discussed.", "contents": "[Recurrent haemoptysis for a period of five years following aspiration of a gall stone]. The article deals with a case report of a 71-years-old female who suffered from hemoptysis over a period of five years. The first hemoptysis occurred postoperatively after a cholecystectomy in December 1966 and was thought to be caused by pulmonary embolism. Persisting episodes of more or less slight hemoptysis in the following years lead to repeated different diagnostic procedures. Radiologically there was an infiltration of the right lower lobe rather constant in size. Endoscopically no bronchial obstruction was visible. In July 1972 the patient suffered from a more severe hemoptoe. X-ray findings were nearly unchanged. With bronchoscopy the hemorrhage could be localized to the segmental bronchi 8 and 9. Right lower lobectomy was done in September 1972 without an exact preoperative diagnosis. The patient had an uneventful postoperative course despite of her age. Pathological examination of the specimen showed a foreign body looking like a gallstone. This diagnosis was confirmed by chemical analysis. To get an explanation we inquired into the circumstances of the cholecystectomy performed 5 years ago. These lead to the conclusion that the concrement must have been vomited and aspirated by the evidently not intubated patient. These suggestions were supported by the fact, that the former operation has been done because of a gangrenous gallbladder with a highly delated common duct and the duodenum, stomach and transverse colon being involved in an inflammatory infiltration. Dangers of asymptomic foreign body aspiration and diagnostic problems in non-opaque peripheral foreign bodies are discussed."}
{"id": "PMID:1079641", "title": "[Intrapulmonary thymoma].", "content": "A primary intrapulmonary left-sided thymoma is described in a 23-year old man. Eight similar observations can be found in the world literature. The histology is quite uniform. The tissue shows characteristic epithelial and lymphatic cellular elements but organoid differentiation into medullar and cortex is lacking. Hassall's granules are only rudimentary. Giant cells in all stages of development, tissue mast cells, varied supporting tissue, epithelial border layer and connective tissue capsule are all present. There is no blastomatous degeneration. Theoretical indications to explain the sudden onset of tendency to proliferation of the ectopic thymus independant of age and sex are discussed.", "contents": "[Intrapulmonary thymoma]. A primary intrapulmonary left-sided thymoma is described in a 23-year old man. Eight similar observations can be found in the world literature. The histology is quite uniform. The tissue shows characteristic epithelial and lymphatic cellular elements but organoid differentiation into medullar and cortex is lacking. Hassall's granules are only rudimentary. Giant cells in all stages of development, tissue mast cells, varied supporting tissue, epithelial border layer and connective tissue capsule are all present. There is no blastomatous degeneration. Theoretical indications to explain the sudden onset of tendency to proliferation of the ectopic thymus independant of age and sex are discussed."}
{"id": "PMID:1079642", "title": "[Complications in mediastinoscopy - a possibility of direct therapy].", "content": "Complications in diagnostic mediastinoscopy are between 0, 7 percent and 1,8 per cent. Lesions of the great vessels end letal in most cases. A possibility is reported how to treat lesions of the tracheobronchial system in emergency cases with lyophilised dura and a histoacryl-adhesive for tissue.", "contents": "[Complications in mediastinoscopy - a possibility of direct therapy]. Complications in diagnostic mediastinoscopy are between 0, 7 percent and 1,8 per cent. Lesions of the great vessels end letal in most cases. A possibility is reported how to treat lesions of the tracheobronchial system in emergency cases with lyophilised dura and a histoacryl-adhesive for tissue."}
{"id": "PMID:1079643", "title": "[Phleborgaphic studies of subclavian vein compression at the shoulder girdle].", "content": "60 healthy persons without clinical signs of neurovascular compression at the shoulder girdle were investigated with phlebograms of the arm under various arm-positions. Venous obstruction could be demonstrated under hyperabduction and shoulder-retraction in 70 per cent of the phlebograms. A compression of the subclavian vein in extreme positions of the arm can therefore be demonstrated in a significant number of healthy individuals. A narrowing of  the costoclavicular space and an impression by the head of the humerus seem tobe the most frequent mechanisms of compression. Repeated compression between clavicula and first rib leads to thickening of the vein wall, forced expiration (Valsalva) to temporary stasis. These mechanisms could be the reason for primary subclavian vein thrombosis in otherwise healthy individuals.", "contents": "[Phleborgaphic studies of subclavian vein compression at the shoulder girdle]. 60 healthy persons without clinical signs of neurovascular compression at the shoulder girdle were investigated with phlebograms of the arm under various arm-positions. Venous obstruction could be demonstrated under hyperabduction and shoulder-retraction in 70 per cent of the phlebograms. A compression of the subclavian vein in extreme positions of the arm can therefore be demonstrated in a significant number of healthy individuals. A narrowing of  the costoclavicular space and an impression by the head of the humerus seem tobe the most frequent mechanisms of compression. Repeated compression between clavicula and first rib leads to thickening of the vein wall, forced expiration (Valsalva) to temporary stasis. These mechanisms could be the reason for primary subclavian vein thrombosis in otherwise healthy individuals."}
{"id": "PMID:1079644", "title": "[Surgical problems in the treatment of aneurysm of femoral artery bifurcation].", "content": "The different types and causes of aneurysm in the region of the femoral artery bifurication as well as the possibilities of surgical treatment are discussed on the basis of 4 patients, each with a differnt type of aneurysm. The examples show clearly that the basic of 4 patients, each with a different type of aneurysm. The examples show clearly that the  basic disease can prevent successful surgical reconstruction of the arterial blood circulation. The use of synthetic prostheses is not possible in case of infection in this area  because of the deleterious subsequences unless the entire infected area can be avoided with a bypass. (Fig. 2a). Trials with autologous veins and arteries, are , therefore, justified. The technical procedure for the use of the greater saphenous vein or deep femoral artery is described. In the authors' patients as well as those reported in the literature, it is clear than aneurysms in the region of the femoral artery bifurcation are sometimes difficult to treat. Hwerver, their localisation near the surface should allow early diagnosis and prompt treatment.", "contents": "[Surgical problems in the treatment of aneurysm of femoral artery bifurcation]. The different types and causes of aneurysm in the region of the femoral artery bifurication as well as the possibilities of surgical treatment are discussed on the basis of 4 patients, each with a differnt type of aneurysm. The examples show clearly that the basic of 4 patients, each with a different type of aneurysm. The examples show clearly that the  basic disease can prevent successful surgical reconstruction of the arterial blood circulation. The use of synthetic prostheses is not possible in case of infection in this area  because of the deleterious subsequences unless the entire infected area can be avoided with a bypass. (Fig. 2a). Trials with autologous veins and arteries, are , therefore, justified. The technical procedure for the use of the greater saphenous vein or deep femoral artery is described. In the authors' patients as well as those reported in the literature, it is clear than aneurysms in the region of the femoral artery bifurcation are sometimes difficult to treat. Hwerver, their localisation near the surface should allow early diagnosis and prompt treatment."}
{"id": "PMID:1079645", "title": "[The development of intramyocardial haemorrhages or fluidaccumulation in the extracellular space].", "content": "Isolated rat hearts according to Langendorff and rabbit hearts after orthostatic collapse were studied under the  light and electron microscope. The light-micrographs were also quantitatively analysed. Changes in the vessels are noticeable, especially those in the sinusoid's. Vessel ruptures occur,  also isolated endothelial ruptures with intact basement membranes and perfusion fluid enters the extracellular space. Numerous 0.06-01 mu large vesicles appear in the cytoplasma of the endothelial cells. A large number of vesiculation processes can be demonstrated along the cell membrane. The extracellular space is strongly-dilated. Changes in the colloid osmotic pressure and a rise in perfusion pressure have no clearly demonstrable influence on the extent of extravasation under the experimental conditions. The mechanism possible inducing such vessel changes is discussed.", "contents": "[The development of intramyocardial haemorrhages or fluidaccumulation in the extracellular space]. Isolated rat hearts according to Langendorff and rabbit hearts after orthostatic collapse were studied under the  light and electron microscope. The light-micrographs were also quantitatively analysed. Changes in the vessels are noticeable, especially those in the sinusoid's. Vessel ruptures occur,  also isolated endothelial ruptures with intact basement membranes and perfusion fluid enters the extracellular space. Numerous 0.06-01 mu large vesicles appear in the cytoplasma of the endothelial cells. A large number of vesiculation processes can be demonstrated along the cell membrane. The extracellular space is strongly-dilated. Changes in the colloid osmotic pressure and a rise in perfusion pressure have no clearly demonstrable influence on the extent of extravasation under the experimental conditions. The mechanism possible inducing such vessel changes is discussed."}
{"id": "PMID:1079646", "title": "[Clinical  use of membrane oxygenators for extended extracroporal circulation].", "content": "Extended perfusion with extracorporal circulation membrane oxygenators of the Lande-Edwards type was performed in 43 patients. Two groups of patients were studied in detail: 1. Patients undergoing aortic valve replacement primarily because of calcified aortic defects (n equals 20; average perfusion time 96 min.1 2. Young children in whom the operation of Mustard was mainly performed (n equals 10; average perfusion time 92 min.) There were no deaths or complications due to the use of the membrane oxygenator. Particular emphasis is laid on the careful preparation of the apparatus. Although venous drainage is only by gravity, high flow perfusion was possible in all age groups. Haemodynamics and metabolism were good even after 1 1/2 hours perfusion. Haemodialysis was slight.", "contents": "[Clinical  use of membrane oxygenators for extended extracroporal circulation]. Extended perfusion with extracorporal circulation membrane oxygenators of the Lande-Edwards type was performed in 43 patients. Two groups of patients were studied in detail: 1. Patients undergoing aortic valve replacement primarily because of calcified aortic defects (n equals 20; average perfusion time 96 min.1 2. Young children in whom the operation of Mustard was mainly performed (n equals 10; average perfusion time 92 min.) There were no deaths or complications due to the use of the membrane oxygenator. Particular emphasis is laid on the careful preparation of the apparatus. Although venous drainage is only by gravity, high flow perfusion was possible in all age groups. Haemodynamics and metabolism were good even after 1 1/2 hours perfusion. Haemodialysis was slight."}
{"id": "PMID:1079647", "title": "[Surgical  problems of the transvenous implantation of cardiac pancemakers].", "content": "489 operation were performed at 323 patients with transvenous implantation of cardiac pacemarkers in 1968-1973. The average of the complications is coming to 25 p.c. of all operations. 41 (34 p.c.) operations of correction are caused by a dislocation of the electrode, 39 (33 p.c.) by a break of the electrode, 18 (14 p.c.) by infectious complications. The operative and technical possibilities to prevent the complications are described. The method of transvenous implantation of cardiac pacemakers is preferred.", "contents": "[Surgical  problems of the transvenous implantation of cardiac pancemakers]. 489 operation were performed at 323 patients with transvenous implantation of cardiac pacemarkers in 1968-1973. The average of the complications is coming to 25 p.c. of all operations. 41 (34 p.c.) operations of correction are caused by a dislocation of the electrode, 39 (33 p.c.) by a break of the electrode, 18 (14 p.c.) by infectious complications. The operative and technical possibilities to prevent the complications are described. The method of transvenous implantation of cardiac pacemakers is preferred."}
{"id": "PMID:1079648", "title": "[Experimental studies on the circulation of the vascular area of the subdiaphragm using a newly developed pulse-wave pump].", "content": "A new developed partial artificial heart system is presented, which allows pulsatile perfusion of vital organs. The results presented reveal the possibility to duplicate pressure curves and peak systolic pressure in the perfused area-similar to physiological preoperative measurements. The kidney function remains intact. Angiographically there is no damage of the kidney vessels evident. The results are compared with datas of other investigators. Especially there opinion about the disturbance of renal flow and function under nonpulsatile conditions are critically evaluated. A possible connnection between the postoperative renal failure after cardio-pulmonary bypass and non-pulsatile perfusion and other pathophysiological mechanisms are discussed.", "contents": "[Experimental studies on the circulation of the vascular area of the subdiaphragm using a newly developed pulse-wave pump]. A new developed partial artificial heart system is presented, which allows pulsatile perfusion of vital organs. The results presented reveal the possibility to duplicate pressure curves and peak systolic pressure in the perfused area-similar to physiological preoperative measurements. The kidney function remains intact. Angiographically there is no damage of the kidney vessels evident. The results are compared with datas of other investigators. Especially there opinion about the disturbance of renal flow and function under nonpulsatile conditions are critically evaluated. A possible connnection between the postoperative renal failure after cardio-pulmonary bypass and non-pulsatile perfusion and other pathophysiological mechanisms are discussed."}
{"id": "PMID:1079649", "title": "[Total electrode retraction (Twiddler syndrome) in infection of the pacemaker system].", "content": "We report upon a case of total retraction of an electrode (Twiddler syndrom) in an infected pacemaker battery. The silk-thread fixation was still  fastened at the right place on the electrode; the tissue owing to the inflammation was no longer sufficient as a fixation point. The casues of this total retraction of the electrode are the following factors: the battery hat sufficient freedom of movement for rotation following exudation into the pacemaker cavity, the electrode was elastic and above all the pacemaker system was infected which could no longer guarantee a sufficient fixation of the electrode in the infected tissue. Attention is drawn to the necessity to remove an infected pacemaker system.", "contents": "[Total electrode retraction (Twiddler syndrome) in infection of the pacemaker system]. We report upon a case of total retraction of an electrode (Twiddler syndrom) in an infected pacemaker battery. The silk-thread fixation was still  fastened at the right place on the electrode; the tissue owing to the inflammation was no longer sufficient as a fixation point. The casues of this total retraction of the electrode are the following factors: the battery hat sufficient freedom of movement for rotation following exudation into the pacemaker cavity, the electrode was elastic and above all the pacemaker system was infected which could no longer guarantee a sufficient fixation of the electrode in the infected tissue. Attention is drawn to the necessity to remove an infected pacemaker system."}
{"id": "PMID:1079652", "title": "Perinatal mortality and maternal mortality at the Provincial Hospital, Quang Ngai, South Vietnam, 1967-1970.", "content": "The perinatal mortality, maternal mortality, infant mortality rates, and the complications of delivery at the Provincial Hospital of Quang Ngai, South Vietnam are described. The perinatal mortality is the only valid statistic available as the infant usually leaves the hospital within three days of delivery. Knowledge pertaining to the 4th to 28th day after birth is scanty and there is insufficient knowledge about the first year of life. Infant mortality is estimated at 277 per 1,000 live births. The perinatal mortality 64.6 per 1,000 live births, and maternal mortality, 106 per 10,000 live births are extremely high in contrast to Western countries. The high perinatal mortality is attributable to deaths during birth, the neonatal and immediate postnatal period. The high maternal mortality is primarily due to caesarean section, anemia, uterine rupture, toxemia, post-partum hemorrhage and puerperal infection.", "contents": "Perinatal mortality and maternal mortality at the Provincial Hospital, Quang Ngai, South Vietnam, 1967-1970. The perinatal mortality, maternal mortality, infant mortality rates, and the complications of delivery at the Provincial Hospital of Quang Ngai, South Vietnam are described. The perinatal mortality is the only valid statistic available as the infant usually leaves the hospital within three days of delivery. Knowledge pertaining to the 4th to 28th day after birth is scanty and there is insufficient knowledge about the first year of life. Infant mortality is estimated at 277 per 1,000 live births. The perinatal mortality 64.6 per 1,000 live births, and maternal mortality, 106 per 10,000 live births are extremely high in contrast to Western countries. The high perinatal mortality is attributable to deaths during birth, the neonatal and immediate postnatal period. The high maternal mortality is primarily due to caesarean section, anemia, uterine rupture, toxemia, post-partum hemorrhage and puerperal infection."}
{"id": "PMID:1079653", "title": "[Enhancement of the cooperation of T- and B-lymphocytes in the immune response and the induction of antibody production in B-mice].", "content": "The effect of poly-4-vinylpyridine and polyacrilic acid on production of antibodyforming cells to the sheep red blood cells in in vivo mixed cultures of T- and B-lymphocytes involved an obvious increase of the immune response. To a lesser extent theimmune response was increased in B-lymphocyte cultures, and no such effect was observed in T-lymphocyte cultures. The poly-4-vinlpyridine and polyacrilic acid were concluded to enhance cooperation between T- and B-lymphocytes and, probably, to replacethe T-lymphocytes function in B-mice.", "contents": "[Enhancement of the cooperation of T- and B-lymphocytes in the immune response and the induction of antibody production in B-mice]. The effect of poly-4-vinylpyridine and polyacrilic acid on production of antibodyforming cells to the sheep red blood cells in in vivo mixed cultures of T- and B-lymphocytes involved an obvious increase of the immune response. To a lesser extent theimmune response was increased in B-lymphocyte cultures, and no such effect was observed in T-lymphocyte cultures. The poly-4-vinlpyridine and polyacrilic acid were concluded to enhance cooperation between T- and B-lymphocytes and, probably, to replacethe T-lymphocytes function in B-mice."}
{"id": "PMID:1079657", "title": "Phytohemagglutinin lymphocyte stimulation in children with recurrent urinary tract infections.", "content": "The delayed hypersensitivity (T cell) response of children with recurrent urinary tract infections, normal children, and children with other bacterial infections was measured by the ability of their lymphocytes to react to vitro to phytohemagglutinin. A comparison among these three groups demonstrated no differences in response. We conclude that the delayed hypersensitivity response is grossly normal in most children with recurrent urinary tract infections.", "contents": "Phytohemagglutinin lymphocyte stimulation in children with recurrent urinary tract infections. The delayed hypersensitivity (T cell) response of children with recurrent urinary tract infections, normal children, and children with other bacterial infections was measured by the ability of their lymphocytes to react to vitro to phytohemagglutinin. A comparison among these three groups demonstrated no differences in response. We conclude that the delayed hypersensitivity response is grossly normal in most children with recurrent urinary tract infections."}
{"id": "PMID:1079673", "title": "[Spinal cord compression in multiple cartilagineous exostoses (case report)].", "content": "A 53-year-old patient with multiple cartilagineous exostoses developed narrowing of the thoracic spinal canal with compression of the cord and spastic paraplegia. The importance of radiologic examination of the whole spine as well as of the extremities is emphasized. When there is an exostosis in the spine neurologic examination disclosing cord compression should be carried out at regular intervals. If there are signs of cord compression, early decompressive laminectomy is the treatment of choice.", "contents": "[Spinal cord compression in multiple cartilagineous exostoses (case report)]. A 53-year-old patient with multiple cartilagineous exostoses developed narrowing of the thoracic spinal canal with compression of the cord and spastic paraplegia. The importance of radiologic examination of the whole spine as well as of the extremities is emphasized. When there is an exostosis in the spine neurologic examination disclosing cord compression should be carried out at regular intervals. If there are signs of cord compression, early decompressive laminectomy is the treatment of choice."}
{"id": "PMID:1079676", "title": "[Multiple pseudoxanthomatour rheumatoid nodules].", "content": "2 cases of Rheumatoid arthritis associated with multiple subcutaneous nodules were presented, which resembled \"pseudoxanthomatour rheumatoid nodules\" as before reported. In the first case, the asymmetrical joint involvement has been preceded by the formation of subcutaneous nodules since 5 years; In the second case, arthritis appeared long before the nodules were formed. In both cases, circumscript vasculitis and high titer of rheumatoid factor were noticed and cystic activities were found in several nodules.", "contents": "[Multiple pseudoxanthomatour rheumatoid nodules]. 2 cases of Rheumatoid arthritis associated with multiple subcutaneous nodules were presented, which resembled \"pseudoxanthomatour rheumatoid nodules\" as before reported. In the first case, the asymmetrical joint involvement has been preceded by the formation of subcutaneous nodules since 5 years; In the second case, arthritis appeared long before the nodules were formed. In both cases, circumscript vasculitis and high titer of rheumatoid factor were noticed and cystic activities were found in several nodules."}
{"id": "PMID:1079679", "title": "[Factors influencing the results of aortocoronary bypass in a homogeneous series of 125 surgical patients].", "content": "A group of 125 patients having undergone aortocoronary bypass by saphenous graft was studied from the point of view of short and medium term results. The following emerged as factors likely to influence these results: --the technique used in the anastamoses and the quality of peroperative myocardial protection; --the presence of hyperlipidaemia; --the restrictive choice of indications in relation to the results of coronary arteriography; --the presence of cardiac insufficiency or an associated valvular lesion. Overall operative mortality was 8 p. cent. Routine ECG's and enzyme studies were carried out to detect postoperative myocardial necrosis. 4 postoperative infarctions without clinical symptoms or signs were detected (2.4 p. cent). 26 patients underwent surgery as an emergency with simultaneous coronary arteriography and surgery in 24. The mortality in this group was not greater (7.6 p. cent) than in the group as a whole. By contrast, mortality was greater in the presence of cardiac insufficiency (25 p. cent) or an associated valvular lesion (16.5 p. cent).", "contents": "[Factors influencing the results of aortocoronary bypass in a homogeneous series of 125 surgical patients]. A group of 125 patients having undergone aortocoronary bypass by saphenous graft was studied from the point of view of short and medium term results. The following emerged as factors likely to influence these results: --the technique used in the anastamoses and the quality of peroperative myocardial protection; --the presence of hyperlipidaemia; --the restrictive choice of indications in relation to the results of coronary arteriography; --the presence of cardiac insufficiency or an associated valvular lesion. Overall operative mortality was 8 p. cent. Routine ECG's and enzyme studies were carried out to detect postoperative myocardial necrosis. 4 postoperative infarctions without clinical symptoms or signs were detected (2.4 p. cent). 26 patients underwent surgery as an emergency with simultaneous coronary arteriography and surgery in 24. The mortality in this group was not greater (7.6 p. cent) than in the group as a whole. By contrast, mortality was greater in the presence of cardiac insufficiency (25 p. cent) or an associated valvular lesion (16.5 p. cent)."}
{"id": "PMID:1079681", "title": "The influence of optokinetic training upon vestibular habituation. Ok-induced modification of vestibular responses elicited by sinusoidal stimuli.", "content": "Unidirectional optokinetic training modifies the response pattern of bidirectional sinusoidal vestibular stimuli (pendular rotation), resulting in an initial response decline. The latter affects only slow phase velocity, whereas the former shows a frequency increase. Optokinetic training obviously has a facilitating effect upon vestibular habituation which cannot be induced by repeated sinusoidal vestibular stimuli alone. Peak frequency increase as well as slow phase velocity decrease of vestibular nystagmus, induced by optokinetic stimuli, are interpreted as a typical transfer mechanism across two sensory systems.", "contents": "The influence of optokinetic training upon vestibular habituation. Ok-induced modification of vestibular responses elicited by sinusoidal stimuli. Unidirectional optokinetic training modifies the response pattern of bidirectional sinusoidal vestibular stimuli (pendular rotation), resulting in an initial response decline. The latter affects only slow phase velocity, whereas the former shows a frequency increase. Optokinetic training obviously has a facilitating effect upon vestibular habituation which cannot be induced by repeated sinusoidal vestibular stimuli alone. Peak frequency increase as well as slow phase velocity decrease of vestibular nystagmus, induced by optokinetic stimuli, are interpreted as a typical transfer mechanism across two sensory systems."}
{"id": "PMID:1079691", "title": "Severe Haemophilus influenzae infections.", "content": "From May 1973 thorugh April 1974, a total of 83 children with severe Haemophilus influenzae infections were treated in three Denver pediatric hospitals. Although meningitis was the most common clinical manifestation (45 cases), other foci of infection were also noted (pneumonia, 12 cases; cheek cellulitis, eight cases; epiglottitis, eight cases; empyema, seven cases; pericarditis, three cases; arthritis, one case; periorbital cellulitis, one case; and abscess, one case). Nine children had positive blood cultures with H influenzae without an initial detectable focus of infection. Two patients developed clinically apparent sites of infection (osteomyelitis and scalp abscesses).", "contents": "Severe Haemophilus influenzae infections. From May 1973 thorugh April 1974, a total of 83 children with severe Haemophilus influenzae infections were treated in three Denver pediatric hospitals. Although meningitis was the most common clinical manifestation (45 cases), other foci of infection were also noted (pneumonia, 12 cases; cheek cellulitis, eight cases; epiglottitis, eight cases; empyema, seven cases; pericarditis, three cases; arthritis, one case; periorbital cellulitis, one case; and abscess, one case). Nine children had positive blood cultures with H influenzae without an initial detectable focus of infection. Two patients developed clinically apparent sites of infection (osteomyelitis and scalp abscesses)."}
{"id": "PMID:1079692", "title": "Pneumocystis carinii pneumonia. Diagnosis by lung biopsy.", "content": "Pneumocystis carinii pneumonia was suspected in 52 patients who underwent lung biopsy for diagnosis of diffuse pulmonary infiltrates. All but five of the procedures were limited thoracotomies. There were no instances of significant postoperative hemorrage or of pneumothorax compromising ventilation. The diagnosis of P. carinii pneumonia was made rapidly from imprints of the lung stained by the Gram-Weigert method in the 18 patients who had P. carinii pneumonia. Over-all, the rate of recovery from Pneumocystis pneumonia after treatment with pentamidine isethionate was 41 percent. However, among patients with no other pulmonary lesion (group A), the survival rate was 58 per cent; none of the patients with coexisting pulmonary disease (group B) recovered. The pulmonary infiltrates in the other 32 patients represented a variety of processes, of which 5 were fungal infections and 5 were due to lymphoma. Fifteen patients had an organizing interstitial and intraalveolar pneumonia of undertermined origin.", "contents": "Pneumocystis carinii pneumonia. Diagnosis by lung biopsy. Pneumocystis carinii pneumonia was suspected in 52 patients who underwent lung biopsy for diagnosis of diffuse pulmonary infiltrates. All but five of the procedures were limited thoracotomies. There were no instances of significant postoperative hemorrage or of pneumothorax compromising ventilation. The diagnosis of P. carinii pneumonia was made rapidly from imprints of the lung stained by the Gram-Weigert method in the 18 patients who had P. carinii pneumonia. Over-all, the rate of recovery from Pneumocystis pneumonia after treatment with pentamidine isethionate was 41 percent. However, among patients with no other pulmonary lesion (group A), the survival rate was 58 per cent; none of the patients with coexisting pulmonary disease (group B) recovered. The pulmonary infiltrates in the other 32 patients represented a variety of processes, of which 5 were fungal infections and 5 were due to lymphoma. Fifteen patients had an organizing interstitial and intraalveolar pneumonia of undertermined origin."}
{"id": "PMID:1079693", "title": "Ocular manifestations of juvenile rheumatoid arthritis.", "content": "We followed 210 cases of juvenile rheumatoid arthritis closely for eleven years. Thirty-six of the 210 patients (17.2%) developed iridocyclitis. Iridocyclitis was seen most frequently in young female patients (0 to 4 years) with the monoarticular or pauciatricular form of the arthritis. However, 30% of the patients developed uveitis after 16 years of age. Although 61% of patients had a noncontributory ocular history on entry, 42% had active uveitis on entry. Our approach was effective in detecting uveitis in new cases and exacerbations of uveitis in established cases. Forty-four percent of patients with uveitis had one or more identifiable signs or symptoms, such as red eye, ocular pain, decreased visual acuity, or photophobia, in order of decreasing frequency. Even after early detection and prompt treatment, 41% of cases of uveitis did not respond to more than six months of intensive topical treatment with corticosteroids and mydriatics. Despite this, there was a dramatic decrease in the 50% incidence of blinding complications of uveitis cited in earlier studies. Cataract and band keratopathy occurred in only 22 and 13% of our group, respectively. We used chloroquine or hydroxychloroquine in 173 of 210 cases and found only one case of chorioretinopathy attributable to these drugs. Systemically administered corticosteroids were used in 75 of 210 cases; a significant number of posterior subcapsular cataracts was found. Typical keratoconjunctivitis sicca developed in three of the uveitis cases. This association with uveitis and JRA was not noted previously. Surgical treatment of cataracts, band keratopathy, and glaucoma achieved uniformly discouraging results.", "contents": "Ocular manifestations of juvenile rheumatoid arthritis. We followed 210 cases of juvenile rheumatoid arthritis closely for eleven years. Thirty-six of the 210 patients (17.2%) developed iridocyclitis. Iridocyclitis was seen most frequently in young female patients (0 to 4 years) with the monoarticular or pauciatricular form of the arthritis. However, 30% of the patients developed uveitis after 16 years of age. Although 61% of patients had a noncontributory ocular history on entry, 42% had active uveitis on entry. Our approach was effective in detecting uveitis in new cases and exacerbations of uveitis in established cases. Forty-four percent of patients with uveitis had one or more identifiable signs or symptoms, such as red eye, ocular pain, decreased visual acuity, or photophobia, in order of decreasing frequency. Even after early detection and prompt treatment, 41% of cases of uveitis did not respond to more than six months of intensive topical treatment with corticosteroids and mydriatics. Despite this, there was a dramatic decrease in the 50% incidence of blinding complications of uveitis cited in earlier studies. Cataract and band keratopathy occurred in only 22 and 13% of our group, respectively. We used chloroquine or hydroxychloroquine in 173 of 210 cases and found only one case of chorioretinopathy attributable to these drugs. Systemically administered corticosteroids were used in 75 of 210 cases; a significant number of posterior subcapsular cataracts was found. Typical keratoconjunctivitis sicca developed in three of the uveitis cases. This association with uveitis and JRA was not noted previously. Surgical treatment of cataracts, band keratopathy, and glaucoma achieved uniformly discouraging results."}
{"id": "PMID:1079694", "title": "Pathologic anatomy of the thalamoperforating arteries in lesions of the third ventricle: Part II.", "content": "A review of vertebral and carotid angiograms in 59 cases of third ventricular pathology demonstrated that the anterior thalamoperforating arteries are affected primarily by obstructive dilatation of the third ventricle, dilatation of the third ventricle secondary to atrophy, intra third ventricular tumors, anterior and para third ventricular tumors, and hypothalamic and thalamic tumors. The posterior thalamoperforating arteries are affected primarily by midbrain atrophy, midbrain tumors, and thalamic tumors. The primary and secondary effects have been outlined. Understanding of the anatomy of the thalamoperforating arteries facilitates localization of the site of obstruction in hydrocephalus, helps differentiate obstructive hydrocephalus from ventricular dilatation secondary to atrophy, permits differentiation between tumoral and non-tumoral dilatation of the third ventricle, aids in the diagnosis and differential diagnosis of intra third and para third ventricular masses including their posterior extent, and facilitates the diagnosis of thalamic, hypothalamic, and midbrain tumors.", "contents": "Pathologic anatomy of the thalamoperforating arteries in lesions of the third ventricle: Part II. A review of vertebral and carotid angiograms in 59 cases of third ventricular pathology demonstrated that the anterior thalamoperforating arteries are affected primarily by obstructive dilatation of the third ventricle, dilatation of the third ventricle secondary to atrophy, intra third ventricular tumors, anterior and para third ventricular tumors, and hypothalamic and thalamic tumors. The posterior thalamoperforating arteries are affected primarily by midbrain atrophy, midbrain tumors, and thalamic tumors. The primary and secondary effects have been outlined. Understanding of the anatomy of the thalamoperforating arteries facilitates localization of the site of obstruction in hydrocephalus, helps differentiate obstructive hydrocephalus from ventricular dilatation secondary to atrophy, permits differentiation between tumoral and non-tumoral dilatation of the third ventricle, aids in the diagnosis and differential diagnosis of intra third and para third ventricular masses including their posterior extent, and facilitates the diagnosis of thalamic, hypothalamic, and midbrain tumors."}
{"id": "PMID:1079696", "title": "Filling defects and nonfilling of the internal auditory canal in posterior fossa myelography.", "content": "A review of posterior fossa myelography at St. Joseph Hospital within the past 5 years is presented. Three false positive diagnoses have been found. Two of the cases were proved to have arachnoiditis, while one demonstrated a knuckle of artery in the canal. One case with large acoustic neuromas causing nonfilling of the contralateral canal is presented. One case with Type I intracanalicular tumor out of 40 acoustic neuromas in the series was diagnosed prior to surgery. The indication for posterior fossa myelography are discussed with a review of the literature.", "contents": "Filling defects and nonfilling of the internal auditory canal in posterior fossa myelography. A review of posterior fossa myelography at St. Joseph Hospital within the past 5 years is presented. Three false positive diagnoses have been found. Two of the cases were proved to have arachnoiditis, while one demonstrated a knuckle of artery in the canal. One case with large acoustic neuromas causing nonfilling of the contralateral canal is presented. One case with Type I intracanalicular tumor out of 40 acoustic neuromas in the series was diagnosed prior to surgery. The indication for posterior fossa myelography are discussed with a review of the literature."}
{"id": "PMID:1079697", "title": "Angiographic diagnosis of intra- and extraaxial tumors in the cerebellopontine angle.", "content": "Angiography is an effective diagnostic modality in the evaluation of patients with suspected cerebellopontine angle tumor, particularly when there is not a clear clinical differentiation between an intraaxial and an extraaxial mass, or when the patient presents with increased intracranial pressure. In a series of 17 extraaxial masses in the cerebellopontine angle and 2 cerebellar tumors which presented into the angle, the most common angiographic findings were: 1. elevation and/or medial displacement of the proximal portions of the superior cerebellar artery; this finding was seen only in the extraaxial tumors and has not been demonstrated with intraaxial masses; 2. downward, medial, and posterior displacement of the proximal portions of the posterior inferior cerebellar artery; 3. lateral displacement and stretching of the hemispheric branches of both the superior cerebellar and posterior inferior cerebellar arteries; and 4. displacement of the vein of the lateral recess and of the petrosal vein either downward and medially or upward and laterally.", "contents": "Angiographic diagnosis of intra- and extraaxial tumors in the cerebellopontine angle. Angiography is an effective diagnostic modality in the evaluation of patients with suspected cerebellopontine angle tumor, particularly when there is not a clear clinical differentiation between an intraaxial and an extraaxial mass, or when the patient presents with increased intracranial pressure. In a series of 17 extraaxial masses in the cerebellopontine angle and 2 cerebellar tumors which presented into the angle, the most common angiographic findings were: 1. elevation and/or medial displacement of the proximal portions of the superior cerebellar artery; this finding was seen only in the extraaxial tumors and has not been demonstrated with intraaxial masses; 2. downward, medial, and posterior displacement of the proximal portions of the posterior inferior cerebellar artery; 3. lateral displacement and stretching of the hemispheric branches of both the superior cerebellar and posterior inferior cerebellar arteries; and 4. displacement of the vein of the lateral recess and of the petrosal vein either downward and medially or upward and laterally."}
{"id": "PMID:1079702", "title": "Acute proliferative retrolental fibroplasia: evolution of the lesion.", "content": "Infants with retrolental fibroplasia have been followed at the Bascom Palmer Eye Institute from August 1969 through December 1974. Changes occurring acute proliferative RLF were documented with fundus photography and fluorescein angiography. These are presented and especially those that have prognostic significance are emphasized.", "contents": "Acute proliferative retrolental fibroplasia: evolution of the lesion. Infants with retrolental fibroplasia have been followed at the Bascom Palmer Eye Institute from August 1969 through December 1974. Changes occurring acute proliferative RLF were documented with fundus photography and fluorescein angiography. These are presented and especially those that have prognostic significance are emphasized."}
{"id": "PMID:1079703", "title": "Cicatricial retrolental fibroplasia: its occurrence without oxygen administration and in full term infants.", "content": "Six patients demonstrating typical signs of the cicatricial phase of retrolental fibroplasia are presented. None of the patients had received supplemental oxygen treatment after birth, and three patients were full term with birth weights ever 6 1/2 pounds. Two of the three premature patients had been born prior to 1901, before the advent of oxygen therapy for premature infants. It is apparent from these observations that retrolental fibroplasia does occur without oxygen administration and also in full term infants.", "contents": "Cicatricial retrolental fibroplasia: its occurrence without oxygen administration and in full term infants. Six patients demonstrating typical signs of the cicatricial phase of retrolental fibroplasia are presented. None of the patients had received supplemental oxygen treatment after birth, and three patients were full term with birth weights ever 6 1/2 pounds. Two of the three premature patients had been born prior to 1901, before the advent of oxygen therapy for premature infants. It is apparent from these observations that retrolental fibroplasia does occur without oxygen administration and also in full term infants."}
{"id": "PMID:1079704", "title": "Retinal detachment in retrolental fibroplasia.", "content": "Retrolental fibroplasia is a continuing problem in ophthalmology and may lead to retinal detachment. In this study, two groups of patients with retrolental fibroplasia and retinal detachment are described. The first group consists of youngsters who develop retinal detachment during their teenage years and frequently have not been diagnosed as having the disease until the retina detaches. Usually definite retinal breaks can be found near the equator and these are round or oval in appearance and without opercula. A second group of patients was noted to develop retinal detachment at an earlier age. The configuration of these detachments suggested a rhegmatogenous etiology, but retinal breaks were hard to detect because the peripheral retina was frequently obscured by a membrane or cataract. Additional evidence to support this opinion was provided when a small retinal hole was identified in one youngster whose ora could be easily seen. Because of the progressive nature of vitreoretinal adhesion in retrolental fibroplasia, it is advocated that youngsters with any evidence of retrolental fibroplasia at the time of discharge from the premature nursery be followed at one month, three months, and six months of age and at four-month intervals thereafter until the age of four years. If no difficulty develops by the time, yearly examinations suffice. Follow-up examinations are important because when prompt diagnosis of retinal detachment is made, the involved eye can often be salvaged with surgery.", "contents": "Retinal detachment in retrolental fibroplasia. Retrolental fibroplasia is a continuing problem in ophthalmology and may lead to retinal detachment. In this study, two groups of patients with retrolental fibroplasia and retinal detachment are described. The first group consists of youngsters who develop retinal detachment during their teenage years and frequently have not been diagnosed as having the disease until the retina detaches. Usually definite retinal breaks can be found near the equator and these are round or oval in appearance and without opercula. A second group of patients was noted to develop retinal detachment at an earlier age. The configuration of these detachments suggested a rhegmatogenous etiology, but retinal breaks were hard to detect because the peripheral retina was frequently obscured by a membrane or cataract. Additional evidence to support this opinion was provided when a small retinal hole was identified in one youngster whose ora could be easily seen. Because of the progressive nature of vitreoretinal adhesion in retrolental fibroplasia, it is advocated that youngsters with any evidence of retrolental fibroplasia at the time of discharge from the premature nursery be followed at one month, three months, and six months of age and at four-month intervals thereafter until the age of four years. If no difficulty develops by the time, yearly examinations suffice. Follow-up examinations are important because when prompt diagnosis of retinal detachment is made, the involved eye can often be salvaged with surgery."}
{"id": "PMID:1079705", "title": "Oxygen toxicity in premature infants.", "content": "With the current state of our knowledge regarding uses, hazards, and dangers, the cavalier offhanded attitude which in the past has all too frequently characterized oxygen use in the premature is no longer acceptable. Sufficient knowledge and technical expertise now exist in spezialized centers which will allow maximal benefit at minimum risk from oxygen usage. Where such centers are readily accessible, the need for oxygen therapy should be considered as a major criterion for transfer from a lesser to a more sophisticated newborn care unit.", "contents": "Oxygen toxicity in premature infants. With the current state of our knowledge regarding uses, hazards, and dangers, the cavalier offhanded attitude which in the past has all too frequently characterized oxygen use in the premature is no longer acceptable. Sufficient knowledge and technical expertise now exist in spezialized centers which will allow maximal benefit at minimum risk from oxygen usage. Where such centers are readily accessible, the need for oxygen therapy should be considered as a major criterion for transfer from a lesser to a more sophisticated newborn care unit."}
{"id": "PMID:1079706", "title": "The role of oxygen in retrolental fibroplasia.", "content": "The effects of oxygen in the immature or incompletely vascularized retina are conveniently divided into an initial vasoconstrictive and obliterative stage and a secondary vasoproliferative response that occurs after removal of the subject from an enriched oxygen environment. Vasoproliferation starts at the zone of perfused and nonperfused retina. The incompletely vascularized retina is uniquely responsive to these oxygen-induced changes. After vascularization is complete and the retinal vessels reach the ora, the susceptibility to hyperoxia disappears. The vessels in the temporal periphery of the retina have a peculiar susceptibility to the primary and secondary effects of hyperoxia on the immature retina. Ophthalmoscopic examination of the temporal periphery therefore should always be done carefully in the premature infant or in older individuals giving a history of prematurity. The indirect ophthalmoscope provides the most satisfactory instrument for examination. The incidence of retrolental fibroplasia has been greatly reduced following the incrimination of oxygen as its principal cause. Further studies are still required to determine precise blood oxygen levels that are safe for the premature retina and to discover other factors that may play a role in the pathogenesis or RLF.", "contents": "The role of oxygen in retrolental fibroplasia. The effects of oxygen in the immature or incompletely vascularized retina are conveniently divided into an initial vasoconstrictive and obliterative stage and a secondary vasoproliferative response that occurs after removal of the subject from an enriched oxygen environment. Vasoproliferation starts at the zone of perfused and nonperfused retina. The incompletely vascularized retina is uniquely responsive to these oxygen-induced changes. After vascularization is complete and the retinal vessels reach the ora, the susceptibility to hyperoxia disappears. The vessels in the temporal periphery of the retina have a peculiar susceptibility to the primary and secondary effects of hyperoxia on the immature retina. Ophthalmoscopic examination of the temporal periphery therefore should always be done carefully in the premature infant or in older individuals giving a history of prematurity. The indirect ophthalmoscope provides the most satisfactory instrument for examination. The incidence of retrolental fibroplasia has been greatly reduced following the incrimination of oxygen as its principal cause. Further studies are still required to determine precise blood oxygen levels that are safe for the premature retina and to discover other factors that may play a role in the pathogenesis or RLF."}
{"id": "PMID:1079710", "title": "Alpha1 antitrypsin phenotypes in Northern Ireland.", "content": "Serum alpha1 antitrypsin levels and phenotypes were examined in 1000 healthy adults in Northern Ireland. The Pi phenotype M accounted for 87-8% Pi MS for 7-3% and Pi MZ for 3-5%. The percentages of the rarer phenotypes were Pi FM 0-3, Pi IM 0-6, Pi SZ 0-4 and Pi Z--0-1. The range of alpha1 antitrypsin levels in different phenotypes showed considerable overlap. Pi MS, Pi MZ and Pi SZ sera gave mean levels of 81%, 61% and 40% respectively of the mean level obtained in Pi M sera and that of the Pi Z--minus serum, 5%. Two distinct types of anomalous pattern were encountered in sera from ten subjects.", "contents": "Alpha1 antitrypsin phenotypes in Northern Ireland. Serum alpha1 antitrypsin levels and phenotypes were examined in 1000 healthy adults in Northern Ireland. The Pi phenotype M accounted for 87-8% Pi MS for 7-3% and Pi MZ for 3-5%. The percentages of the rarer phenotypes were Pi FM 0-3, Pi IM 0-6, Pi SZ 0-4 and Pi Z--0-1. The range of alpha1 antitrypsin levels in different phenotypes showed considerable overlap. Pi MS, Pi MZ and Pi SZ sera gave mean levels of 81%, 61% and 40% respectively of the mean level obtained in Pi M sera and that of the Pi Z--minus serum, 5%. Two distinct types of anomalous pattern were encountered in sera from ten subjects."}
{"id": "PMID:1079711", "title": "Simplified medium for ampicillin susceptibility testing of Haemophilus influenzae.", "content": "Recent reports of bona fide ampicillin resistance among strains of Haemophilus influenzae have emphasized the need for improved methods of susceptibility testing of clinical isolates. A simplified medium composed of only Mueller-Hinton medium plus supplement C (yeast autolysate and hematin) was used successfully for ampicillin susceptibility testing of 20 recent clinical isolates of H. influenzae, including six strains with confirmed ampicillin resistance. With this medium, susceptible strains were inhibited by less than 1 mug of ampicillin per ml, whereas resistant strains had minimal inhibitory concentrations of 8 mug or greater per ml. This medium can be used for either disk diffusion, tube dilution, or agar plate dilution methods of susceptibility testing. It has the practical advantage of simplicity of preparation and retains the light color and transparency of Mueller-Hinton medium. This medium can also be used for preparation of an inoculum suspension that can be adjusted to a standardized turbidity.", "contents": "Simplified medium for ampicillin susceptibility testing of Haemophilus influenzae. Recent reports of bona fide ampicillin resistance among strains of Haemophilus influenzae have emphasized the need for improved methods of susceptibility testing of clinical isolates. A simplified medium composed of only Mueller-Hinton medium plus supplement C (yeast autolysate and hematin) was used successfully for ampicillin susceptibility testing of 20 recent clinical isolates of H. influenzae, including six strains with confirmed ampicillin resistance. With this medium, susceptible strains were inhibited by less than 1 mug of ampicillin per ml, whereas resistant strains had minimal inhibitory concentrations of 8 mug or greater per ml. This medium can be used for either disk diffusion, tube dilution, or agar plate dilution methods of susceptibility testing. It has the practical advantage of simplicity of preparation and retains the light color and transparency of Mueller-Hinton medium. This medium can also be used for preparation of an inoculum suspension that can be adjusted to a standardized turbidity."}
{"id": "PMID:1079712", "title": "Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance.", "content": "Strains of Haemophilus influenzae type b sporadically isolated from clinical specimens are ampicillin resistant due to production of a beta-lactamase. This enzyme which inactivates ampicillin and penicillin G is not produced by ampicillin-susceptible strains. Various characteristics of beta-lactamase production and ampicillin resistance of three H. influenzae type b isolates were investigated. A sensitive iodometric test was employed to detect beta-lactamase; positive results were obtained in 5 min with 10(9) bacteria taken from cultures on a nutritionally adequate agar medium. This simple chemical test will enable the hospital laboratory to obtain presumptive evidence of ampicillin resistance on the same day that H. influenzae is isolated.", "contents": "Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance. Strains of Haemophilus influenzae type b sporadically isolated from clinical specimens are ampicillin resistant due to production of a beta-lactamase. This enzyme which inactivates ampicillin and penicillin G is not produced by ampicillin-susceptible strains. Various characteristics of beta-lactamase production and ampicillin resistance of three H. influenzae type b isolates were investigated. A sensitive iodometric test was employed to detect beta-lactamase; positive results were obtained in 5 min with 10(9) bacteria taken from cultures on a nutritionally adequate agar medium. This simple chemical test will enable the hospital laboratory to obtain presumptive evidence of ampicillin resistance on the same day that H. influenzae is isolated."}
{"id": "PMID:1079715", "title": "Use of the activated coagulation time in intraoperative heparin reversal for cardiopulmonary operations.", "content": "Activated clotting time (ACT) was used in 300 consecutive patients undergoing cardiac operations to determine the adequacy of heparin reversal. Mean ACT prior to protamine sulfate administration was 9 minutes 40 seconds. A return to normal value (less than 2 min 10 sec) occurred in three-fourths of our patients following administration of 1.5 mg of protamine sulfate for each 100 units of heparin. Additional protamine sulfate was administered in 50 mg doses to those having abnormal ACT until normal clotting was obtained. Normal values for ACT usually coincided with clotting in the operative field. ACT proved to be a reliable guide to protamine sulfate administration.", "contents": "Use of the activated coagulation time in intraoperative heparin reversal for cardiopulmonary operations. Activated clotting time (ACT) was used in 300 consecutive patients undergoing cardiac operations to determine the adequacy of heparin reversal. Mean ACT prior to protamine sulfate administration was 9 minutes 40 seconds. A return to normal value (less than 2 min 10 sec) occurred in three-fourths of our patients following administration of 1.5 mg of protamine sulfate for each 100 units of heparin. Additional protamine sulfate was administered in 50 mg doses to those having abnormal ACT until normal clotting was obtained. Normal values for ACT usually coincided with clotting in the operative field. ACT proved to be a reliable guide to protamine sulfate administration."}
{"id": "PMID:1079716", "title": "Beneficial effect of human growth hormone on stress ulcers.", "content": "Human growth hormone was effective in healing erosions and controlling hemorrhage in six of eight patients with stress ulcers. This approach was based on known beneficial effects of growth hormone on nucleic acid and protein synthesis, demonstration of deleterious effects of stress on nucleic acid and protein synthesis, and demonstrated protective effects of growth hormone on animals subjected to stress. Bleeding cessation within 24 hours of its administration in two patients suggests the possible role of additional mechanisms involved in hemostasis. In a comparable group of eight patients with stress ulcer hemorrhage not treated with this agent, six died with continued bleeding. This high mortality represented the usual outcome in our patients with stress ulcer hemorrhage. These observations need to be extended to additional patients with stress ulcers in a larger randomized study.", "contents": "Beneficial effect of human growth hormone on stress ulcers. Human growth hormone was effective in healing erosions and controlling hemorrhage in six of eight patients with stress ulcers. This approach was based on known beneficial effects of growth hormone on nucleic acid and protein synthesis, demonstration of deleterious effects of stress on nucleic acid and protein synthesis, and demonstrated protective effects of growth hormone on animals subjected to stress. Bleeding cessation within 24 hours of its administration in two patients suggests the possible role of additional mechanisms involved in hemostasis. In a comparable group of eight patients with stress ulcer hemorrhage not treated with this agent, six died with continued bleeding. This high mortality represented the usual outcome in our patients with stress ulcer hemorrhage. These observations need to be extended to additional patients with stress ulcers in a larger randomized study."}
{"id": "PMID:1079717", "title": "Peroneal nerve stimulator in rehabilitation of hemiplegic patients.", "content": "Of nine hemiplegic patients having foot-drop who were test-treated with the Philips functional electronic peroneal stimulator, only three who tolerated continued use of the stimulator for five weeks had improvement in function. Findings in these cases indicate that to be selected for trial use of a stimulator a hemiplegic patient should fulfill the following criteria: (1) be in the hospital, (2) be ambulant with or without a cane and/or short leg brace, (3) have no severe contracture of the ankle joint, (4) have no severe spasticity, (5) have no obvious lower motor neuron lesions, (6) be cooperative and intelligent, (7) have no severe manual difficulty (he should be able to put on the stimulator without assistance), and (8) be able to tolerate the discomfort from the stimulation.", "contents": "Peroneal nerve stimulator in rehabilitation of hemiplegic patients. Of nine hemiplegic patients having foot-drop who were test-treated with the Philips functional electronic peroneal stimulator, only three who tolerated continued use of the stimulator for five weeks had improvement in function. Findings in these cases indicate that to be selected for trial use of a stimulator a hemiplegic patient should fulfill the following criteria: (1) be in the hospital, (2) be ambulant with or without a cane and/or short leg brace, (3) have no severe contracture of the ankle joint, (4) have no severe spasticity, (5) have no obvious lower motor neuron lesions, (6) be cooperative and intelligent, (7) have no severe manual difficulty (he should be able to put on the stimulator without assistance), and (8) be able to tolerate the discomfort from the stimulation."}
{"id": "PMID:1079718", "title": "Peroneal nerve conduction velocity after chronic electrical stimulation.", "content": "The effects of electric stimulation on nerve conduction velocity of the peroneal nerve via surgically implanted electrodes was evaluated in a group of nine hemiplegic patients. After a mean duration of 25 months nerve conduction velocity averaged 47.3 meters/sec in the uninvolved and 46.1 meters/sec in the stimulated (paretic) extremity. In a control group of ten hemiplegic subjects who did not receive stimulation, nerve conduction velocity averaged 43.1 meters/sec in the hemiplegic leg, and 41.3 meters/sec in the uninvolved leg. These differences were not statistically significant. Maximum dorsiflexion strength was increased in all patients following chronic stimulation.", "contents": "Peroneal nerve conduction velocity after chronic electrical stimulation. The effects of electric stimulation on nerve conduction velocity of the peroneal nerve via surgically implanted electrodes was evaluated in a group of nine hemiplegic patients. After a mean duration of 25 months nerve conduction velocity averaged 47.3 meters/sec in the uninvolved and 46.1 meters/sec in the stimulated (paretic) extremity. In a control group of ten hemiplegic subjects who did not receive stimulation, nerve conduction velocity averaged 43.1 meters/sec in the hemiplegic leg, and 41.3 meters/sec in the uninvolved leg. These differences were not statistically significant. Maximum dorsiflexion strength was increased in all patients following chronic stimulation."}
{"id": "PMID:1079719", "title": "Problem oriented medical records: their impact on staff communication, attitudes and decision making.", "content": "A comparative, two-stage between hospital study involving university rehabilitation centers was undertaken to evaluate the impact of the problem oriented medical record (POMR) on staff communication, attitude change and decision making. Staff characteristics varied from one center to another. The semantic differential component of the project did not reveal any change due to the POMR in the staff perception of the medical record, the health care team or the various health professions. However, team function with respect to the management of specific patients improved in the centers which implemented the POMR during the time period of the project.", "contents": "Problem oriented medical records: their impact on staff communication, attitudes and decision making. A comparative, two-stage between hospital study involving university rehabilitation centers was undertaken to evaluate the impact of the problem oriented medical record (POMR) on staff communication, attitude change and decision making. Staff characteristics varied from one center to another. The semantic differential component of the project did not reveal any change due to the POMR in the staff perception of the medical record, the health care team or the various health professions. However, team function with respect to the management of specific patients improved in the centers which implemented the POMR during the time period of the project."}
{"id": "PMID:1079721", "title": "Levodopa-induced myoclonus.", "content": "Twelve parkinsonian patients on long-term levodopa therapy developed intermittent, myoclonic body jerks. The movements consisted of single unilateral or bilateral abrupt jerks of the extremities and occurred most frequently during sleep. Although directly related to daily dosage of levodopa, the myoclonus was specifically blocked by the serotonin antagonist, methysergide. Levodopa-induced myoclonus may be related to intermittent increases of activity of serotonin in the brain and results from levodopa-induced dysregulation of serotonin activity.", "contents": "Levodopa-induced myoclonus. Twelve parkinsonian patients on long-term levodopa therapy developed intermittent, myoclonic body jerks. The movements consisted of single unilateral or bilateral abrupt jerks of the extremities and occurred most frequently during sleep. Although directly related to daily dosage of levodopa, the myoclonus was specifically blocked by the serotonin antagonist, methysergide. Levodopa-induced myoclonus may be related to intermittent increases of activity of serotonin in the brain and results from levodopa-induced dysregulation of serotonin activity."}
{"id": "PMID:1079722", "title": "[Intraosseous injections and the osseous pressure in patients with rheumatoid shoulder pain].", "content": "Intraosseous injection of physiological saline or local anaesthetic (prilocain) results in a marked relief of rest pain in rheumatoid shoulder. 28 shoulders were treated in tis way and the intraosseous pressure of the proximal end of the humerus was measured with simple central venous pressure equipment. The intraosseous pressure tends to decrease after injections.", "contents": "[Intraosseous injections and the osseous pressure in patients with rheumatoid shoulder pain]. Intraosseous injection of physiological saline or local anaesthetic (prilocain) results in a marked relief of rest pain in rheumatoid shoulder. 28 shoulders were treated in tis way and the intraosseous pressure of the proximal end of the humerus was measured with simple central venous pressure equipment. The intraosseous pressure tends to decrease after injections."}
{"id": "PMID:1079724", "title": "Coronary artery reperfusion: effects of occlusion duration on reactive hyperemia responses.", "content": "In 24 unanesthetized dogs, we monitored reactive hyperemia responses in the coronary vascular bed after different durations of coronary occlusion and during coronary artery reperfusion. After two hours of occlusion, no significant changes in functional capacity occurred, evidenced by unchanged peak reactive hyperemia, reactive hyperemia flow and repayment of flow debt responses. With occlusions of six hours or more, these responses were impaired upon reperfusion, and remained diminished for up to five days. In those animals reperfused after two and six hours of coronary occlusion, myocardial infarcts were significantly smaller than those seen in animals reperfused after 24 to 72 hours of occlusion (4%, 14%, 20% and 21%, respectively). Thus, the effectiveness of any reperfusion procedure depends on its early institution when the coronary vascular bed has minimal change in functional capacity and the size of the infarct can still be reduced.", "contents": "Coronary artery reperfusion: effects of occlusion duration on reactive hyperemia responses. In 24 unanesthetized dogs, we monitored reactive hyperemia responses in the coronary vascular bed after different durations of coronary occlusion and during coronary artery reperfusion. After two hours of occlusion, no significant changes in functional capacity occurred, evidenced by unchanged peak reactive hyperemia, reactive hyperemia flow and repayment of flow debt responses. With occlusions of six hours or more, these responses were impaired upon reperfusion, and remained diminished for up to five days. In those animals reperfused after two and six hours of coronary occlusion, myocardial infarcts were significantly smaller than those seen in animals reperfused after 24 to 72 hours of occlusion (4%, 14%, 20% and 21%, respectively). Thus, the effectiveness of any reperfusion procedure depends on its early institution when the coronary vascular bed has minimal change in functional capacity and the size of the infarct can still be reduced."}
{"id": "PMID:1079727", "title": "Immunoglobulin synthesis by peripheral blood cells in systemic lupus erythematosus.", "content": "A study of the immunoglobulin (Ig) synthesis by peripheral blood cells of patients with systemic lupus erythematosus (SLE), rheumatoid arthritis, and controls was carried out. Synthesis was markedly elevated in patients with active SLE, whereas patients with the active SLE or RA showed normal values. Treatment of 3 patients with active SLE resulted in a reduction of Ig synthesis that correlated well with the disappearance of disease activity. There results indicate that the abnormal immune process in active SLE, characterized by intense B-lymphocyte stimulation, is associated with the appearance of more highly differentiated B cells in the blood.", "contents": "Immunoglobulin synthesis by peripheral blood cells in systemic lupus erythematosus. A study of the immunoglobulin (Ig) synthesis by peripheral blood cells of patients with systemic lupus erythematosus (SLE), rheumatoid arthritis, and controls was carried out. Synthesis was markedly elevated in patients with active SLE, whereas patients with the active SLE or RA showed normal values. Treatment of 3 patients with active SLE resulted in a reduction of Ig synthesis that correlated well with the disappearance of disease activity. There results indicate that the abnormal immune process in active SLE, characterized by intense B-lymphocyte stimulation, is associated with the appearance of more highly differentiated B cells in the blood."}
{"id": "PMID:1079728", "title": "Cell-mediated immunity in rheumatic diseases. II. Mitogen responses in RA, SLE, and other illnesses: correlation with T- and B-lymphocyte populations.", "content": "Lymphocyte responses to phytohemagglutinin, concanavalin A, and pokeweed mitogen were tested in normal patients and in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), scleroderma (PSS), other connective tissue disease, and other illnesses. The relationship of lymphocyte response to diagnosis, therapy, and T- and B-lymphocyte populations was analyzed. Additional studies included the determination of proliferative responses of various combinations of purified T and B lymphocytes cultured with plant mitogens. Lymphocytes from patients with RA and SLE incorporated significantly less thymidine in the presence of plant mitogens as compared to normal and comparably ill subjects. Treatment had no effect on mitogen response. Responses to all three mitogens correlated closely in patients with RA, SLE, or PSS; no correlation was noted between the response to mitogen of lymphocytes in culture and the number of T cells cultured.", "contents": "Cell-mediated immunity in rheumatic diseases. II. Mitogen responses in RA, SLE, and other illnesses: correlation with T- and B-lymphocyte populations. Lymphocyte responses to phytohemagglutinin, concanavalin A, and pokeweed mitogen were tested in normal patients and in patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), scleroderma (PSS), other connective tissue disease, and other illnesses. The relationship of lymphocyte response to diagnosis, therapy, and T- and B-lymphocyte populations was analyzed. Additional studies included the determination of proliferative responses of various combinations of purified T and B lymphocytes cultured with plant mitogens. Lymphocytes from patients with RA and SLE incorporated significantly less thymidine in the presence of plant mitogens as compared to normal and comparably ill subjects. Treatment had no effect on mitogen response. Responses to all three mitogens correlated closely in patients with RA, SLE, or PSS; no correlation was noted between the response to mitogen of lymphocytes in culture and the number of T cells cultured."}
{"id": "PMID:1079734", "title": "Review of variations in origin of left circumflex coronary artery.", "content": "During the performance of 200 routine coronary angiograms, 5 patients with anatomical variants of the origin of the left circumflex coronary artery were found. The origin and course of the left circumflex coronary artery is described in each case. The practical importance of these variant vessels in patients being considered for coronary artery surgery is emphasized.", "contents": "Review of variations in origin of left circumflex coronary artery. During the performance of 200 routine coronary angiograms, 5 patients with anatomical variants of the origin of the left circumflex coronary artery were found. The origin and course of the left circumflex coronary artery is described in each case. The practical importance of these variant vessels in patients being considered for coronary artery surgery is emphasized."}
{"id": "PMID:1079731", "title": "[The clinical problem of hereditary hemorrhagic diseases developing prolonged bleeding time].", "content": "Thirty-two patients with hereditary hemorrhagic diseases and a platelet functional abnormality were set apart from our group of patients with hereditary hemorrhagic diseases, and their symptoms, signs and hematological examinations were collected; the initial events and the age of the patients when they were obsserved, the main hemorrhagic manifestations during their clinical course, the clinical severity of the disorders, the survival of the patients and the laboratory test for hemostasis useful to make the diagnosis, were evaluated. In reference to bleeding time, thirty patients had abnormal bleeding time, but the other two had normal bleeding time.", "contents": "[The clinical problem of hereditary hemorrhagic diseases developing prolonged bleeding time]. Thirty-two patients with hereditary hemorrhagic diseases and a platelet functional abnormality were set apart from our group of patients with hereditary hemorrhagic diseases, and their symptoms, signs and hematological examinations were collected; the initial events and the age of the patients when they were obsserved, the main hemorrhagic manifestations during their clinical course, the clinical severity of the disorders, the survival of the patients and the laboratory test for hemostasis useful to make the diagnosis, were evaluated. In reference to bleeding time, thirty patients had abnormal bleeding time, but the other two had normal bleeding time."}
{"id": "PMID:1079732", "title": "[Kartagener's syndrome in childhood. Report of 2 cases].", "content": "Two cases of children with the syndrome (triad) of Kartagener are described. One of them presented two of the triad's components; the other one, probably complete, as a part of a partial revision of clinical charts, corresponding to children with \"situs inversus\", from 1970 to December 1973, at the Children's Hospital of Mexico. The literature is reviewed, concerning this unfrequently seen entity, finding a great majority of reports in children, less than 14 years old, a positive family history and serious respiratory disease complications on those affected. It is important to understand the early diagnosis and treatment of these patients, mainly if they have upper respiratory disease, to avoid future onset of bronchiectasis. The genetical aspects, as well as variable clinical affections are mentioned.", "contents": "[Kartagener's syndrome in childhood. Report of 2 cases]. Two cases of children with the syndrome (triad) of Kartagener are described. One of them presented two of the triad's components; the other one, probably complete, as a part of a partial revision of clinical charts, corresponding to children with \"situs inversus\", from 1970 to December 1973, at the Children's Hospital of Mexico. The literature is reviewed, concerning this unfrequently seen entity, finding a great majority of reports in children, less than 14 years old, a positive family history and serious respiratory disease complications on those affected. It is important to understand the early diagnosis and treatment of these patients, mainly if they have upper respiratory disease, to avoid future onset of bronchiectasis. The genetical aspects, as well as variable clinical affections are mentioned."}
{"id": "PMID:1079736", "title": "The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase.", "content": "The mode of inhibition by alpha-1-antitrypsin of chymotrypsin, trypsin and pancreatic elastase was examined by a kinetic method. All three enzymes were completely bound to alpha-1-antitrypsin; therefore, the dissociation constant of the enzyme-inhibitor complex was too low to measure with these methods. The dissociation constants for the three enzyme-inhibitor complexes were estimated to be less than 5 - 10 minus 9 M. However, alpha-1-antitrypsin could be specifically displaced from Sepharose-bound elastase with an irreversible inhibitor of that enzyme. Additional experiments showed that dioxane, 20% (v/v), blocked 100% of the inhibition of elastase, 16% of the inhibition of trypsin, and 0% of the inhibition of chymotrypsin. The effect was reversed by diluting the dioxane to 1% (v/v). These findings indicated that alpha-1-antitrypsin was tightly bound to the three enzymes studied but did not allow discrimination as to the nature of the inhibition. However, the competitive mode of inhibition was suggested by the displacement of alpha-1-antitrypsin from elastase by an irreversible inhibitor of the enzyme that bound covalently at the enzyme-active site. The variable susceptibility of the enzyme to blockage of the inhibition by low concentrations of p-dioxane suggested that hydrophobic bonds may be important in the interaction with alpha-1-antitrypsin.", "contents": "The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase. The mode of inhibition by alpha-1-antitrypsin of chymotrypsin, trypsin and pancreatic elastase was examined by a kinetic method. All three enzymes were completely bound to alpha-1-antitrypsin; therefore, the dissociation constant of the enzyme-inhibitor complex was too low to measure with these methods. The dissociation constants for the three enzyme-inhibitor complexes were estimated to be less than 5 - 10 minus 9 M. However, alpha-1-antitrypsin could be specifically displaced from Sepharose-bound elastase with an irreversible inhibitor of that enzyme. Additional experiments showed that dioxane, 20% (v/v), blocked 100% of the inhibition of elastase, 16% of the inhibition of trypsin, and 0% of the inhibition of chymotrypsin. The effect was reversed by diluting the dioxane to 1% (v/v). These findings indicated that alpha-1-antitrypsin was tightly bound to the three enzymes studied but did not allow discrimination as to the nature of the inhibition. However, the competitive mode of inhibition was suggested by the displacement of alpha-1-antitrypsin from elastase by an irreversible inhibitor of the enzyme that bound covalently at the enzyme-active site. The variable susceptibility of the enzyme to blockage of the inhibition by low concentrations of p-dioxane suggested that hydrophobic bonds may be important in the interaction with alpha-1-antitrypsin."}
{"id": "PMID:1079738", "title": "Antinuclear antibodies in psychiatric illness: their relationship to diagnosis and drug treatment.", "content": "Antinuclear antibodies occurred more often and in higher titres in psychiatric patients than in controls. Anti-DNA antibodies were not found. We suggest that antinuclear antibodies may be drug-induced and that lithium carbonate may have a particular tendency to produce this reaction.", "contents": "Antinuclear antibodies in psychiatric illness: their relationship to diagnosis and drug treatment. Antinuclear antibodies occurred more often and in higher titres in psychiatric patients than in controls. Anti-DNA antibodies were not found. We suggest that antinuclear antibodies may be drug-induced and that lithium carbonate may have a particular tendency to produce this reaction."}
{"id": "PMID:1079741", "title": "Natamycin in genital candidosis in men.", "content": "In a trial of natamycin, an antifungal antibiotic in a vanishing cream base, assessment was possible in 66 men with genital or anal candidosis. The overall cure rate was 82 percent, In 43 patients with culturally proven candidosis it was 98 percent. but in 23 patients treated solely on clinical impression it was only 52 percent. Symptoms were rapidly relieved in those who responded and there were no side-effects. In our hands, natamycin 2 per cent cream has proved to be a valuable preparation in the treatment of candidal balanitis.", "contents": "Natamycin in genital candidosis in men. In a trial of natamycin, an antifungal antibiotic in a vanishing cream base, assessment was possible in 66 men with genital or anal candidosis. The overall cure rate was 82 percent, In 43 patients with culturally proven candidosis it was 98 percent. but in 23 patients treated solely on clinical impression it was only 52 percent. Symptoms were rapidly relieved in those who responded and there were no side-effects. In our hands, natamycin 2 per cent cream has proved to be a valuable preparation in the treatment of candidal balanitis."}
{"id": "PMID:1079743", "title": "Regulation of 5-hydroxytryptamine metabolism in mouse brain by adrenal glucocorticoids.", "content": "The effects of glucocorticoid hormone on the metabolism of brain 5-hydroxytryptamine (5-HT) were studied in mice. A single injection of hydrocortisone acetate (HCA; 20 mg/kg, i.p.) accelerated the accumulation of 5-HT in whole brain after inhibition of monoamine oxidase activity by paragyline. The hormone did not appear to change brain tryptophan hydroxylase or 5-hydroxytryptophan decarboxylase activity. However, tryptophan levels in brain were elevated by 50% within 1 h after treatment with HCA. The effect of HCA on brain tryptophan levels was localized mainly in the nerve endings. In vitro synaptosomal preparations, HCA at 10(-5)-10(-7)M or corticosterone at 10(-5) M was found to stimulate the uptake of L-[3H]-tryptophan by the synaptosomes while androgenic and progesterone-like steroids were ineffective. These results demonstrate that glucocorticoids may directly act on nerve terminals in the regulation of 5-HT synthesis through an action on the uptake of tryptophan.", "contents": "Regulation of 5-hydroxytryptamine metabolism in mouse brain by adrenal glucocorticoids. The effects of glucocorticoid hormone on the metabolism of brain 5-hydroxytryptamine (5-HT) were studied in mice. A single injection of hydrocortisone acetate (HCA; 20 mg/kg, i.p.) accelerated the accumulation of 5-HT in whole brain after inhibition of monoamine oxidase activity by paragyline. The hormone did not appear to change brain tryptophan hydroxylase or 5-hydroxytryptophan decarboxylase activity. However, tryptophan levels in brain were elevated by 50% within 1 h after treatment with HCA. The effect of HCA on brain tryptophan levels was localized mainly in the nerve endings. In vitro synaptosomal preparations, HCA at 10(-5)-10(-7)M or corticosterone at 10(-5) M was found to stimulate the uptake of L-[3H]-tryptophan by the synaptosomes while androgenic and progesterone-like steroids were ineffective. These results demonstrate that glucocorticoids may directly act on nerve terminals in the regulation of 5-HT synthesis through an action on the uptake of tryptophan."}
{"id": "PMID:1079745", "title": "The effects of morphine and halothane anaesthesia on urine norepinephrine during and after coronary artery surgery.", "content": "Urine concentrations and excretion rates of norepinephrine were measured in 28 patients anaesthetized with halothane or morphine before, during and for two hours after aortocoronary artery grafting procedures. All patients were paralyzed with d-tubocurarine, intubated and respiration was controlled. Urine was obtained for 90 minutes before induction, during induction, before, during and after bypass and postoperatively. In patients anaesthetized with halothane, urinary norepinephrine concentrations and excretion rates were not significantly different from preoperative values until the postoperative period. Patients anaesthetized with morphine did not have urine norepinephrine concentrations different from preoperative values until bypass, when they became significantly increased. All subsequent urine norepinephrine concentrations and excretion rates were significantly elevated when compared to preoperative values. These findings do not indicate a mechanism but they do demonstrate that morphine anaesthesia is associated with increased urinary and probably also increased plasma levels of norepinephrine during and after cardiopulmonary bypass in patients undergoing coronary artery operations.", "contents": "The effects of morphine and halothane anaesthesia on urine norepinephrine during and after coronary artery surgery. Urine concentrations and excretion rates of norepinephrine were measured in 28 patients anaesthetized with halothane or morphine before, during and for two hours after aortocoronary artery grafting procedures. All patients were paralyzed with d-tubocurarine, intubated and respiration was controlled. Urine was obtained for 90 minutes before induction, during induction, before, during and after bypass and postoperatively. In patients anaesthetized with halothane, urinary norepinephrine concentrations and excretion rates were not significantly different from preoperative values until the postoperative period. Patients anaesthetized with morphine did not have urine norepinephrine concentrations different from preoperative values until bypass, when they became significantly increased. All subsequent urine norepinephrine concentrations and excretion rates were significantly elevated when compared to preoperative values. These findings do not indicate a mechanism but they do demonstrate that morphine anaesthesia is associated with increased urinary and probably also increased plasma levels of norepinephrine during and after cardiopulmonary bypass in patients undergoing coronary artery operations."}
{"id": "PMID:1079746", "title": "Studies on the integrative activity of the vestibular nuclei complex.", "content": "Central mechanisms currently used in clinical vestibular tests are far more complex than generally thought. The first synaptic station, the vestibular nuclei, represents a sensory-motor centre integrating vestibular impulses with proprioceptive afferents from muscles and joints. This convergence can be found on cells with long axons projecting to the oculo-motor system, to the spinal cord and to the thalamus as well as on cells without long projecting axons. There are also vestibulo-motor cells transmitting pure vestibular information.", "contents": "Studies on the integrative activity of the vestibular nuclei complex. Central mechanisms currently used in clinical vestibular tests are far more complex than generally thought. The first synaptic station, the vestibular nuclei, represents a sensory-motor centre integrating vestibular impulses with proprioceptive afferents from muscles and joints. This convergence can be found on cells with long axons projecting to the oculo-motor system, to the spinal cord and to the thalamus as well as on cells without long projecting axons. There are also vestibulo-motor cells transmitting pure vestibular information."}
{"id": "PMID:1079760", "title": "LDH isozymes of human T and B lymphocytes.", "content": "Peripheral lymphocytes were obtained from five normal healthy adults. T and B lymphocytes were separated by rosette formation. LDH (L-lactate: NAD-+ oxidoreductase, EC 1.1.1.27) isozymes were studied on both populations after agar gel electrophoresis. The mean B lymphocyte pattern was: LDH-1, 7.2%; LDH-2, 25.0%; LDH-3, 39.2%; LDH-4, 21.9%; LDH-5, 6.6%. The mean T lymphocyte pattern was: LDH-1, 25.8%; LDH-2, 35.1%; LDH-3, 28.3%; LDH-4, 9.4%, LDH-5, 1.3%. This makes the T lymphocytes look more aerobic than the B lymphocytes. Daudicells had the following pattern: LDH-1, 8.4%; LDH-2, 19.1%; LDH-3, 26.6%, LDH-4, 24.9%; LDH-5, 20.9%.", "contents": "LDH isozymes of human T and B lymphocytes. Peripheral lymphocytes were obtained from five normal healthy adults. T and B lymphocytes were separated by rosette formation. LDH (L-lactate: NAD-+ oxidoreductase, EC 1.1.1.27) isozymes were studied on both populations after agar gel electrophoresis. The mean B lymphocyte pattern was: LDH-1, 7.2%; LDH-2, 25.0%; LDH-3, 39.2%; LDH-4, 21.9%; LDH-5, 6.6%. The mean T lymphocyte pattern was: LDH-1, 25.8%; LDH-2, 35.1%; LDH-3, 28.3%; LDH-4, 9.4%, LDH-5, 1.3%. This makes the T lymphocytes look more aerobic than the B lymphocytes. Daudicells had the following pattern: LDH-1, 8.4%; LDH-2, 19.1%; LDH-3, 26.6%, LDH-4, 24.9%; LDH-5, 20.9%."}
{"id": "PMID:1079768", "title": "The two human trypsinogens. Inhibition spectra of the two human trypsins derived from their purified zymogens.", "content": "The two human anionic trypsinogens 1 and 2 were purified from human pancreatic juice by gel filtration on Sephadex G-100 and by chromatography on DEAE-cellulose. After activation of their respective zymogens by porcine enterokinase, human trypsins 1 and 2 were studied for their reaction with a wide variety of proteinase inhibitors. Kunitz pancreatic trypsin inhibitor and human pancreatic secretory trypsin inhibitor completely inhibited both human trypsins at a stoichiometric inhibitor-to-enzyme ratio of one to one. In contrast, bovine pancreatic secretory trypsin inhibitor (Kazal's inhibitor) failed to inhibit either human trypsin. The inhibition of both human trypsins by porcine pancreatic secretory trypsin inhibitor was demonstrated. The reactions of the trypsins with chicken ovomucoid, Ascaris lumbricoides (type suis), human sperm and blood plasma trypsin inhibitors were studied. The most striking difference between the two human trypsins was the reaction with soybean trypsin inhibitor (Kunitz). Trypsin 2 was completely inhibited in a one-to-one molar ratio while trypsin 1 was poorly inhibited. The presence of a prekallikrein in human pancreatic juice is discussed.", "contents": "The two human trypsinogens. Inhibition spectra of the two human trypsins derived from their purified zymogens. The two human anionic trypsinogens 1 and 2 were purified from human pancreatic juice by gel filtration on Sephadex G-100 and by chromatography on DEAE-cellulose. After activation of their respective zymogens by porcine enterokinase, human trypsins 1 and 2 were studied for their reaction with a wide variety of proteinase inhibitors. Kunitz pancreatic trypsin inhibitor and human pancreatic secretory trypsin inhibitor completely inhibited both human trypsins at a stoichiometric inhibitor-to-enzyme ratio of one to one. In contrast, bovine pancreatic secretory trypsin inhibitor (Kazal's inhibitor) failed to inhibit either human trypsin. The inhibition of both human trypsins by porcine pancreatic secretory trypsin inhibitor was demonstrated. The reactions of the trypsins with chicken ovomucoid, Ascaris lumbricoides (type suis), human sperm and blood plasma trypsin inhibitors were studied. The most striking difference between the two human trypsins was the reaction with soybean trypsin inhibitor (Kunitz). Trypsin 2 was completely inhibited in a one-to-one molar ratio while trypsin 1 was poorly inhibited. The presence of a prekallikrein in human pancreatic juice is discussed."}
{"id": "PMID:1079769", "title": "Studies on bacteriophage M13 DNA. 1. A cleavage map of the M13 genome.", "content": "A physical map of the bacteriophage M13 genome has been constructed on the basis of specific cleavage of M13 replicative form DNA by bacterial restriction endonucleases. The 13 fragments produced by the enzyme from Haemophilus aphirophilus (endonuclease R.Hap II) as well as the 10 fragments produced by the enzyme from Haemophilus aegyptius (endonuclease R.Hae III) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. In addition, the single site in M13 replicative form DNA cleaved by the restriction enzyme from Haemophilus influenzae Rd (endonuclease R.Hin dII) has been located more precisely. With this unique site as a reference point, the H. aphirophilus cleavage sites and the H. aegyptius cleavage sites have been localized on the map.", "contents": "Studies on bacteriophage M13 DNA. 1. A cleavage map of the M13 genome. A physical map of the bacteriophage M13 genome has been constructed on the basis of specific cleavage of M13 replicative form DNA by bacterial restriction endonucleases. The 13 fragments produced by the enzyme from Haemophilus aphirophilus (endonuclease R.Hap II) as well as the 10 fragments produced by the enzyme from Haemophilus aegyptius (endonuclease R.Hae III) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. In addition, the single site in M13 replicative form DNA cleaved by the restriction enzyme from Haemophilus influenzae Rd (endonuclease R.Hin dII) has been located more precisely. With this unique site as a reference point, the H. aphirophilus cleavage sites and the H. aegyptius cleavage sites have been localized on the map."}
{"id": "PMID:1079780", "title": "[Pregnancy in teenagers (author's transl)].", "content": "In this study pregnancy, delivery and the newborn infant of 151 juvenile primiparas and 1034 adolescent primiparas were analyzed and were compared with a group of 3563 primiparas 21, 23 and 25 years of age. Regarding the obstetric history and the intrapartum period there was no increase incidence of complications among the young patients, however we found among the older women an increase in the incidence of operative deliveries. In the postpartum period it was noted that the young primiparas had a higher incidence of postpartum bleeding caused by atonia of the uterus and also an increased incidence of placental complications with a high rate of manual placental extraction and curettages. A positive correlation was found between premature birth and perinatal mortality and the younger the age of the mother. The newborn of the young mothers was also lighter and shorter at birth and had a higher incidence of malformations. This study has shown, that juvenile expectant mothers are a risk group for obstetricians and pediatricians.", "contents": "[Pregnancy in teenagers (author's transl)]. In this study pregnancy, delivery and the newborn infant of 151 juvenile primiparas and 1034 adolescent primiparas were analyzed and were compared with a group of 3563 primiparas 21, 23 and 25 years of age. Regarding the obstetric history and the intrapartum period there was no increase incidence of complications among the young patients, however we found among the older women an increase in the incidence of operative deliveries. In the postpartum period it was noted that the young primiparas had a higher incidence of postpartum bleeding caused by atonia of the uterus and also an increased incidence of placental complications with a high rate of manual placental extraction and curettages. A positive correlation was found between premature birth and perinatal mortality and the younger the age of the mother. The newborn of the young mothers was also lighter and shorter at birth and had a higher incidence of malformations. This study has shown, that juvenile expectant mothers are a risk group for obstetricians and pediatricians."}
{"id": "PMID:1079787", "title": "Thymus lymphocytes. Efflux and restoration phases after peripheral exposure of mice to phytohaemagglutinin.", "content": "Juvenile Swiss mice experienced significant diminutions in thymic content of both small, non-proliferating and larger, proliferating lymphocytes within 12 hours after intraperitoneal injection of phytohaemagglutinin. These alterations, which may predate alterations in lymphocyte traffic and morphology elsewhere, were interpreted as being the consequence of a massive thymus lymphocyte emigration, not of in situ cytolysis, and were effaced by intrathymic lymphocyte restoration toward control numbers by 24 hours.", "contents": "Thymus lymphocytes. Efflux and restoration phases after peripheral exposure of mice to phytohaemagglutinin. Juvenile Swiss mice experienced significant diminutions in thymic content of both small, non-proliferating and larger, proliferating lymphocytes within 12 hours after intraperitoneal injection of phytohaemagglutinin. These alterations, which may predate alterations in lymphocyte traffic and morphology elsewhere, were interpreted as being the consequence of a massive thymus lymphocyte emigration, not of in situ cytolysis, and were effaced by intrathymic lymphocyte restoration toward control numbers by 24 hours."}
{"id": "PMID:1079788", "title": "Cell-mediated immune responses in Staphylococcus aureus infections in mice.", "content": "Delayed hypersensitivity to staphylococcal antigens was shown in mice repeatedly infected with Staphylococcus aureus. It was characterized by footpad swelling at 48 hours with a mononuclear cell infiltrate and could be transferred to non-infected recipients by T lymphocytes from infected animals, but not by serum. Recipients of immune T cells produced very severe necrotic lesions when challenged with staphylococci. This was in contrast to the protection against necrosis in recipients afforded by serum from infected donors. When both serum and cells were transferred into the same mouse the humoral effects overshadowed or perhaps inhibited those mediated by cells with resultant protection against staphylococcal dermonecrosis.", "contents": "Cell-mediated immune responses in Staphylococcus aureus infections in mice. Delayed hypersensitivity to staphylococcal antigens was shown in mice repeatedly infected with Staphylococcus aureus. It was characterized by footpad swelling at 48 hours with a mononuclear cell infiltrate and could be transferred to non-infected recipients by T lymphocytes from infected animals, but not by serum. Recipients of immune T cells produced very severe necrotic lesions when challenged with staphylococci. This was in contrast to the protection against necrosis in recipients afforded by serum from infected donors. When both serum and cells were transferred into the same mouse the humoral effects overshadowed or perhaps inhibited those mediated by cells with resultant protection against staphylococcal dermonecrosis."}
{"id": "PMID:1079789", "title": "Anti-DNA Investigated the DNA bentonite flocculation technique.", "content": "The DNA bentonite flocculation test was used to investigate the presence of antibody to DNA. In most sera a prozone-like phenomenon was found, caused by a non-specific inhibition of serum. Anti-DNA was found with extreme frequency in patients with SLE, chronic liver disease, acute hepatitis, infectious mononucleosis, and blood donors over the age of 60, but was found rarely or not at all in patients with some other disease or blood donors under 60 years of age.", "contents": "Anti-DNA Investigated the DNA bentonite flocculation technique. The DNA bentonite flocculation test was used to investigate the presence of antibody to DNA. In most sera a prozone-like phenomenon was found, caused by a non-specific inhibition of serum. Anti-DNA was found with extreme frequency in patients with SLE, chronic liver disease, acute hepatitis, infectious mononucleosis, and blood donors over the age of 60, but was found rarely or not at all in patients with some other disease or blood donors under 60 years of age."}
{"id": "PMID:1079791", "title": "Cell-mediated cytotoxicity to moloney sarcoma in syngeneic and allogeneic rats.", "content": "Cell-mediated cytotoxicity in the primary immune response to Moloney sarcoma tumor (MST) in allogeneic and syngeneic rats was found to be predominantly T-cell-dependent. A minor non-T-cell cytotoxic activity may also have been detected. CMC was presumably directed against tumor and viral related antigens in the syngeneic host and primarily against alloantigens in the allogeneic host. CMC was more virgorous in the syngeneic host. This may be due to differences in quantities or immunogenicities of the various antigens involved. Two peaks of T-cells in effector populations were observed during a 20-day post-inoculation period. The first peak corresponded to peak T-cell-mediated cytotoxicity on day 8 and the second peak occurred on days 13 or 14 when CMC was minimal or undetectable.", "contents": "Cell-mediated cytotoxicity to moloney sarcoma in syngeneic and allogeneic rats. Cell-mediated cytotoxicity in the primary immune response to Moloney sarcoma tumor (MST) in allogeneic and syngeneic rats was found to be predominantly T-cell-dependent. A minor non-T-cell cytotoxic activity may also have been detected. CMC was presumably directed against tumor and viral related antigens in the syngeneic host and primarily against alloantigens in the allogeneic host. CMC was more virgorous in the syngeneic host. This may be due to differences in quantities or immunogenicities of the various antigens involved. Two peaks of T-cells in effector populations were observed during a 20-day post-inoculation period. The first peak corresponded to peak T-cell-mediated cytotoxicity on day 8 and the second peak occurred on days 13 or 14 when CMC was minimal or undetectable."}
{"id": "PMID:1079792", "title": "Cellular basis for the immune response to methylcholanthrene-induced tumors in mice. Heterogeneity of effector cells.", "content": "Immune resistance to methylcholanthrene-induced tumors has two phases, an early specific and a late non-specific phase. Both phases were found to be T-cell-dependent in vivo. Thus, adult thymectomized, irradiated, bone-marrow-protected mice bearing H-1 tumor isografts showed impaired resistance to challenge with homologous (H-1) and heterologous (H-3) tumor cells. In each case resistance was restored by injection of thymus cells. In vitro analysis of the cellular basis of resistance revealed that different mechanisms were involved in the two phases. The cytotoxic effect of immune spleen cells taken during the early specific phase was inhibited by pretreatment with anti-O serum and complement and by removal of macrophages. Neither procedure, however, interfered with the cytotoxic potential of immune spleen cells taken during the late non-specific phage of immunity. Passage of immune spleen cells through rabbit-IgG anti-mouse immunoglobulin-coated columns (which yielded a T-cell-enriched, B-cell-depleted population) resulted in abrogration of cytotoxicity whether the cells were obtained during the early or the late phase of resistance. The inability of late-phase spleen cells to kill was explicable in terms of B-cell removal since T-cells and macrophages had been shown to be ineffective at that time. In contrast, the failure of column-treated cells from the early phase to kill was found to be due to removal of adherent cells rather than B-cells since cytotoxicity (1) was abrogated by passage through control columns coated with rabbit-IgG anti-sheep red blood cell antibody which did not retain B-cells and (2) could be restored by addition of immune macrophages (from anti-O serum-treated spleens). Taken together, these results indicate that the cellular basis of immune resistance to methylcholanthrene-induced tumors is heterogeneous. The early specific phase seems to be mediated by an interaction between T-cells and macrophages; the late none-specific phase, although T-cell dependent in its induction, depends on a different effector mechanism, possibly involving a cell or its products of the B lineage.", "contents": "Cellular basis for the immune response to methylcholanthrene-induced tumors in mice. Heterogeneity of effector cells. Immune resistance to methylcholanthrene-induced tumors has two phases, an early specific and a late non-specific phase. Both phases were found to be T-cell-dependent in vivo. Thus, adult thymectomized, irradiated, bone-marrow-protected mice bearing H-1 tumor isografts showed impaired resistance to challenge with homologous (H-1) and heterologous (H-3) tumor cells. In each case resistance was restored by injection of thymus cells. In vitro analysis of the cellular basis of resistance revealed that different mechanisms were involved in the two phases. The cytotoxic effect of immune spleen cells taken during the early specific phase was inhibited by pretreatment with anti-O serum and complement and by removal of macrophages. Neither procedure, however, interfered with the cytotoxic potential of immune spleen cells taken during the late non-specific phage of immunity. Passage of immune spleen cells through rabbit-IgG anti-mouse immunoglobulin-coated columns (which yielded a T-cell-enriched, B-cell-depleted population) resulted in abrogration of cytotoxicity whether the cells were obtained during the early or the late phase of resistance. The inability of late-phase spleen cells to kill was explicable in terms of B-cell removal since T-cells and macrophages had been shown to be ineffective at that time. In contrast, the failure of column-treated cells from the early phase to kill was found to be due to removal of adherent cells rather than B-cells since cytotoxicity (1) was abrogated by passage through control columns coated with rabbit-IgG anti-sheep red blood cell antibody which did not retain B-cells and (2) could be restored by addition of immune macrophages (from anti-O serum-treated spleens). Taken together, these results indicate that the cellular basis of immune resistance to methylcholanthrene-induced tumors is heterogeneous. The early specific phase seems to be mediated by an interaction between T-cells and macrophages; the late none-specific phase, although T-cell dependent in its induction, depends on a different effector mechanism, possibly involving a cell or its products of the B lineage."}
{"id": "PMID:1079793", "title": "Induction of specific changes in the surface membrane of myeloid leukemic cells by steroid hormones.", "content": "Normal mature macrophages and granulocytes have surface membrane receptors for specific immunoglobulin and immunoglobulin complement, which can be detected by rosette formation with erythrocytes coated with antibody (EA) or with antibody and complement (EAC). There are three types of myeloid leukemia cells, IR-+D-+, IR-+D-minus and IR-minus D-minus. IR-+D-+ cells were induced to form receptors for EAC but not- for EA by the steroid hormones prednisolone, dexamethasone and estradiol. Induction required protein synthesis and was not inhibited by cordycepin or vinblastine. Optimum induction required the continued presence of the hormones. IR-+D-+ cells were also induced by these hormones to migrate in agar, attach to the surface of a Petri dish and form macrophages. IR-+D-minus cells showed a lower inducibility by these hormones and no formation of macrophages. There was no induction of any of these changes with IR-minusD-minus cells. The steroid hormones progesterone, testosterone and cortisone did not induce these changes in any of the leukemic cells and inhibited induction by prednisolone, dexamethasone and estradiol. The results indicate that specific surface membrane changes in myeloid leukemic cells can be induced by certain steroid hormones.", "contents": "Induction of specific changes in the surface membrane of myeloid leukemic cells by steroid hormones. Normal mature macrophages and granulocytes have surface membrane receptors for specific immunoglobulin and immunoglobulin complement, which can be detected by rosette formation with erythrocytes coated with antibody (EA) or with antibody and complement (EAC). There are three types of myeloid leukemia cells, IR-+D-+, IR-+D-minus and IR-minus D-minus. IR-+D-+ cells were induced to form receptors for EAC but not- for EA by the steroid hormones prednisolone, dexamethasone and estradiol. Induction required protein synthesis and was not inhibited by cordycepin or vinblastine. Optimum induction required the continued presence of the hormones. IR-+D-+ cells were also induced by these hormones to migrate in agar, attach to the surface of a Petri dish and form macrophages. IR-+D-minus cells showed a lower inducibility by these hormones and no formation of macrophages. There was no induction of any of these changes with IR-minusD-minus cells. The steroid hormones progesterone, testosterone and cortisone did not induce these changes in any of the leukemic cells and inhibited induction by prednisolone, dexamethasone and estradiol. The results indicate that specific surface membrane changes in myeloid leukemic cells can be induced by certain steroid hormones."}
{"id": "PMID:1079794", "title": "Photoreactivation of bacteriophage phi chi 174.", "content": "U.V.-irradiated single-stranded DNA-bacteriophage phi chi 174 shows a photoreactivable sector of 0-17. That this sector is relatively small compared with those for the double-stranded DNA-phages T2 and T6 (about 0-5) does not necessarily seem to be due to the pyrimidine dimers in single-stranded DNA being intrinsically a poorer substrate for the photoreactivating enzyme. This follows from the observation that intracellularly irradiated single-stranded phi chi 174 DNA also shows a photoreactivable sector of 0-4 to 0-5. The same value is obtained for intracellularly irradiated double-stranded phi chi 174 DNA. Photoreactivation of intracellularly irradiated single-stranded phi chi 174 DNA is not constant with U.V. dose for the lower dose ranged. Possible explanations for this are discussed.", "contents": "Photoreactivation of bacteriophage phi chi 174. U.V.-irradiated single-stranded DNA-bacteriophage phi chi 174 shows a photoreactivable sector of 0-17. That this sector is relatively small compared with those for the double-stranded DNA-phages T2 and T6 (about 0-5) does not necessarily seem to be due to the pyrimidine dimers in single-stranded DNA being intrinsically a poorer substrate for the photoreactivating enzyme. This follows from the observation that intracellularly irradiated single-stranded phi chi 174 DNA also shows a photoreactivable sector of 0-4 to 0-5. The same value is obtained for intracellularly irradiated double-stranded phi chi 174 DNA. Photoreactivation of intracellularly irradiated single-stranded phi chi 174 DNA is not constant with U.V. dose for the lower dose ranged. Possible explanations for this are discussed."}
{"id": "PMID:1079795", "title": "Effect of X-irradiation on 3-H-glucosamine uptake by human embryo fibroblasts.", "content": "The incorporation of 3-H-glucosamine into unirradiated and X-irradiated (905 rad) cultures of human embryo fibroblasts was studied by light microscopic, autoradiographic and biochemical methods in pulse-chase experiments. The precursor was 3-H-glucosamine. In unirradiated cells the grains appeared in various subcellular regions in a particular order: at first over the Golgi complex, then over the whole cytoplasm and finally at least some of the labelled macromolecules emerged from the cell. The radioactivity emerging from the cell appeared to be protein bound. Due to the effect of irradiation, the uptake of 3-H-glucosamine by the interphase cells was considerably decreased. The amount of radioactivity per cell indicated both the inhibition of cytoplasmic segregation and migration of labelled macromolecules from the cell. In the mitotic figures appearing after the release from radiation-induced mitotic block (approximately 30 hours after irradiation), the grain counts were still considerably lower than the controls. The functional and structural radiation injury of the Golgi-complex was suggested by the less well-defined sequence of localization of radioactive macromolecules than in control cells.", "contents": "Effect of X-irradiation on 3-H-glucosamine uptake by human embryo fibroblasts. The incorporation of 3-H-glucosamine into unirradiated and X-irradiated (905 rad) cultures of human embryo fibroblasts was studied by light microscopic, autoradiographic and biochemical methods in pulse-chase experiments. The precursor was 3-H-glucosamine. In unirradiated cells the grains appeared in various subcellular regions in a particular order: at first over the Golgi complex, then over the whole cytoplasm and finally at least some of the labelled macromolecules emerged from the cell. The radioactivity emerging from the cell appeared to be protein bound. Due to the effect of irradiation, the uptake of 3-H-glucosamine by the interphase cells was considerably decreased. The amount of radioactivity per cell indicated both the inhibition of cytoplasmic segregation and migration of labelled macromolecules from the cell. In the mitotic figures appearing after the release from radiation-induced mitotic block (approximately 30 hours after irradiation), the grain counts were still considerably lower than the controls. The functional and structural radiation injury of the Golgi-complex was suggested by the less well-defined sequence of localization of radioactive macromolecules than in control cells."}
{"id": "PMID:1079796", "title": "Lymphocyte depletion in peripheral blood of acutely gamma-irradiated rats.", "content": "Groups of albino rats were exposed to 200, 800, 2000 and 5000 R of 60-Co gamma-radiation. Peripheral blood samples were taken from the tail at 1, 2, 4, 8 and 24 hours after irradiation. Calculation of the lymphocyte count per unit blood volume was made for each sample. Control rats were also mock-irradiated and sampled in the same manner. The time response of the lymphocyte count indicated that within the first hour, there was a drastic prompt drop. From one to eight hours after irradiation, the counts declined continuously, and from eight to twenty-four hours less rapidly. The depletion rates for the one to eight hour period were calculated and found to be statistically different for all groups, except between 2000 and 5000 R.", "contents": "Lymphocyte depletion in peripheral blood of acutely gamma-irradiated rats. Groups of albino rats were exposed to 200, 800, 2000 and 5000 R of 60-Co gamma-radiation. Peripheral blood samples were taken from the tail at 1, 2, 4, 8 and 24 hours after irradiation. Calculation of the lymphocyte count per unit blood volume was made for each sample. Control rats were also mock-irradiated and sampled in the same manner. The time response of the lymphocyte count indicated that within the first hour, there was a drastic prompt drop. From one to eight hours after irradiation, the counts declined continuously, and from eight to twenty-four hours less rapidly. The depletion rates for the one to eight hour period were calculated and found to be statistically different for all groups, except between 2000 and 5000 R."}
{"id": "PMID:1079797", "title": "Chromosomes and cellular radiosensitivity. IV. A new radiotaxon represented by the ferns.", "content": "Survival curves were constructed and D0 values determined after X-irradiation of single-celled germinating spores of 14 species of ferns, which had a wide variation in interphase chromosome and nuclear volumes and in DNA content per chromosome and per nucleus. A good fit to a line of slope equal to -1 is given on a log-log plot relating D0 to interphase chromosome volume. In a previous publication (Sparrow, Underbrink and Sparrow 1967), ferns fell into radiotaxon VIII, but the new data suggest that they should be assigned to a new group (VIIa) falling midway between radiotaxa VII and VIII. The calculated energy absorption per chromosome at D0 for VIIa is also intermediate between those for VII and VIII. If the value of this parameter is set at 1 for radiotaxon V, that for VIIa is approximately 32 times greater and the radiotaxa V, VI, VII and VIIa form a series 1 : 4 : 14 : 32. The new results indicate that radiotaxon VIII may have to be abandoned unless some other radiobiologically unexplored group (e.g. algae) fills the gap.", "contents": "Chromosomes and cellular radiosensitivity. IV. A new radiotaxon represented by the ferns. Survival curves were constructed and D0 values determined after X-irradiation of single-celled germinating spores of 14 species of ferns, which had a wide variation in interphase chromosome and nuclear volumes and in DNA content per chromosome and per nucleus. A good fit to a line of slope equal to -1 is given on a log-log plot relating D0 to interphase chromosome volume. In a previous publication (Sparrow, Underbrink and Sparrow 1967), ferns fell into radiotaxon VIII, but the new data suggest that they should be assigned to a new group (VIIa) falling midway between radiotaxa VII and VIII. The calculated energy absorption per chromosome at D0 for VIIa is also intermediate between those for VII and VIII. If the value of this parameter is set at 1 for radiotaxon V, that for VIIa is approximately 32 times greater and the radiotaxa V, VI, VII and VIIa form a series 1 : 4 : 14 : 32. The new results indicate that radiotaxon VIII may have to be abandoned unless some other radiobiologically unexplored group (e.g. algae) fills the gap."}
{"id": "PMID:1079798", "title": "Prophage inductive efficiency of alkylating agents and radiations.", "content": "The prophage inducing efficiency in E. coli K-12 (lambda) of a number of agents--alkylating agents and radiations--has been compared at a high survival of bacteria. The inducing effectiveness (per alkylation in DNA) and efficiency (compared with mutation frequency in E. coli Sd-4) was found to decrease in the order 2-hydroxyethylating agents (2-hydroxyethyl methanesulphonate and ethylene oxide) greater than isopropyl methanesulphonate approximately equal to methyl methanesulphonate greater than ethylating agents (diethyl sulphate, ethyl methanesulphonate). The low inducing activity of the ethylating agents could not be explained with respect to their reactivity towards targets of differing nucleophilicity, nor could the effectiveness in mutagenicity or the ability to break the chromosomes in the presence of metal ions be invoked. The high inducing efficiency of hydroxyalkylating agents may be related to their ability to break DNA strands.", "contents": "Prophage inductive efficiency of alkylating agents and radiations. The prophage inducing efficiency in E. coli K-12 (lambda) of a number of agents--alkylating agents and radiations--has been compared at a high survival of bacteria. The inducing effectiveness (per alkylation in DNA) and efficiency (compared with mutation frequency in E. coli Sd-4) was found to decrease in the order 2-hydroxyethylating agents (2-hydroxyethyl methanesulphonate and ethylene oxide) greater than isopropyl methanesulphonate approximately equal to methyl methanesulphonate greater than ethylating agents (diethyl sulphate, ethyl methanesulphonate). The low inducing activity of the ethylating agents could not be explained with respect to their reactivity towards targets of differing nucleophilicity, nor could the effectiveness in mutagenicity or the ability to break the chromosomes in the presence of metal ions be invoked. The high inducing efficiency of hydroxyalkylating agents may be related to their ability to break DNA strands."}
{"id": "PMID:1079799", "title": "Effect of whole-body irradiation on transcription in the rat thymus.", "content": "Studies were carried out to elucidate the mechanism underlying the impairment in RNA synthesis in the rat thymus after whole-body exposure to X-rays (1000 rad). The ability of thymus nuclei to catalyse RNA polymerization in vitro was adversely affected in irradiated rats. Further experiments showed that whole-body irradiation caused a considerable decrease in both the activity of RNA polymerase and the template efficiency of chromatin but not in that of DNA isolated from chromatin. These radiation-induced changes in transcription seem to arise partly from the direct effect of radiation on the thymus itself and partly from the abscopal mechanism(s).", "contents": "Effect of whole-body irradiation on transcription in the rat thymus. Studies were carried out to elucidate the mechanism underlying the impairment in RNA synthesis in the rat thymus after whole-body exposure to X-rays (1000 rad). The ability of thymus nuclei to catalyse RNA polymerization in vitro was adversely affected in irradiated rats. Further experiments showed that whole-body irradiation caused a considerable decrease in both the activity of RNA polymerase and the template efficiency of chromatin but not in that of DNA isolated from chromatin. These radiation-induced changes in transcription seem to arise partly from the direct effect of radiation on the thymus itself and partly from the abscopal mechanism(s)."}
{"id": "PMID:1079800", "title": "Chain reactions and radiosensitization: model enzyme studies.", "content": "The influence of persulphate ions on the radiosensitivity of dilute aqueous solutions of ribonuclease and lysozyme has been studied under a variety of conditions. Rate-constants for reactions of the sulphate radical, SO4, with a variety of solutes and transient product spectra of some of these reactions are reported. The presence of persulphate ions in general enhances the radiation sensitivity; in nitrous-oxide-saturated persulphate solution, a chain reaction has been demonstrated.", "contents": "Chain reactions and radiosensitization: model enzyme studies. The influence of persulphate ions on the radiosensitivity of dilute aqueous solutions of ribonuclease and lysozyme has been studied under a variety of conditions. Rate-constants for reactions of the sulphate radical, SO4, with a variety of solutes and transient product spectra of some of these reactions are reported. The presence of persulphate ions in general enhances the radiation sensitivity; in nitrous-oxide-saturated persulphate solution, a chain reaction has been demonstrated."}
{"id": "PMID:1079804", "title": "Methylase activities from Haemophilus influenzae that protect Haemophilus parainfluenzae transforming deoxyribonucleic acid from inactivation by Haemophilus influenzae endonuclease R.", "content": "Specific methylases that have the properties of deoxyribonucleic acid (DNA) modification enzymes have been isolated from Haemophilus influenzae strain Rd. Two activities ((Methylase IIa and methylase III) were found to protect transforming DNA of H. parainfluenzae from the action of H. influenzae restriction enzymes. To determine the specificty of the protection, a procedure based on biological activity was developed for the separation and purification of the restriction endonucleases from H. influenzae strain Rd. Two endonuclease R activities presumably corresponding to Hind II and Hind III (P. H. Roy and H. O. Smith, 1973; H. O. Smith and K. W. Wilcox, 1970) were characterized by differences in their chromatographic properties, ability to attack T7 DNA, and inactivation of the transforming activity of different markers of H. parainfluenzae DNA. One endonuclease R enzyme (Hind II) attacked T7 DNA and was found to inactivate the dalacin resistance marker (smaller than 0.01% activity remaining) with only a slight effect on the streptomycin resistance marker (83% activity remaining). Methylase IIa treatment protected 40% of the dalacin resistance marker of H. parainfluenzae DNA from inactivation by Hind II. The other restriction activity (Hind III) was inert towards T7 DNA and inactivated the streptomycin resistance marker of H. parainfluenzae DNA (smaller than 0.01% activity remaining) without any effect on the dalacin resistance marker. The methylation of H. parainfluenzae DNA accomplished by methylase III protected 60% of the transforming activity of the streptomycin resistance marker of H. parainfluenzae DNA from the action of Hind III.", "contents": "Methylase activities from Haemophilus influenzae that protect Haemophilus parainfluenzae transforming deoxyribonucleic acid from inactivation by Haemophilus influenzae endonuclease R. Specific methylases that have the properties of deoxyribonucleic acid (DNA) modification enzymes have been isolated from Haemophilus influenzae strain Rd. Two activities ((Methylase IIa and methylase III) were found to protect transforming DNA of H. parainfluenzae from the action of H. influenzae restriction enzymes. To determine the specificty of the protection, a procedure based on biological activity was developed for the separation and purification of the restriction endonucleases from H. influenzae strain Rd. Two endonuclease R activities presumably corresponding to Hind II and Hind III (P. H. Roy and H. O. Smith, 1973; H. O. Smith and K. W. Wilcox, 1970) were characterized by differences in their chromatographic properties, ability to attack T7 DNA, and inactivation of the transforming activity of different markers of H. parainfluenzae DNA. One endonuclease R enzyme (Hind II) attacked T7 DNA and was found to inactivate the dalacin resistance marker (smaller than 0.01% activity remaining) with only a slight effect on the streptomycin resistance marker (83% activity remaining). Methylase IIa treatment protected 40% of the dalacin resistance marker of H. parainfluenzae DNA from inactivation by Hind II. The other restriction activity (Hind III) was inert towards T7 DNA and inactivated the streptomycin resistance marker of H. parainfluenzae DNA (smaller than 0.01% activity remaining) without any effect on the dalacin resistance marker. The methylation of H. parainfluenzae DNA accomplished by methylase III protected 60% of the transforming activity of the streptomycin resistance marker of H. parainfluenzae DNA from the action of Hind III."}
{"id": "PMID:1079805", "title": "Phosphorylation and dephosphorylation of frog rod outer segment membranes as part of the visual process.", "content": "The light-activated phosphorylation of rod outer segment membranes may be an intermediary process controlling photoreceptor responses. We have measured phosphorylation of the opsin moiety of rhodopsin in isolated frog retinas and in rod outer segment suspensions and have demonstrated a phosphorylation-dephosphorylation sequence in the suspensions. The results indicate that these reactions take place in vivo and may be physiologically relevant. Extraction of a protein kinase activity from rod outer segment membranes renders the membranes incapable of phosphorylation, but the light-activated reaction can be reconstituted by mixing the soluble extract and the \"depleted\" membranes. Illumination of the extract is without effect. Thus the light activation mechanism resides in the membranes. Regeneration of rhodopsin from opsin and 11-cis retinal does not influence the phosphorylation. Once activated, the reaction may use either rhodopsin or opsin as the substrate. Furthermore, 11-cis retinal regenerates rhodopsin from phosphorylated opsin without releasing bound phosphate. The isolated rod outer segment which contains regenerated rhodopsin thus differs from one that is dark adapted in that phosphate can remain bound and the phosphorylation reaction remains activated. Dark adaptation in vivo must include at least two membrane associated reactions beyond regeneration of rhodopsin's spectral properties: dephosphorylation, and the inactivation of the phosphorylation process.", "contents": "Phosphorylation and dephosphorylation of frog rod outer segment membranes as part of the visual process. The light-activated phosphorylation of rod outer segment membranes may be an intermediary process controlling photoreceptor responses. We have measured phosphorylation of the opsin moiety of rhodopsin in isolated frog retinas and in rod outer segment suspensions and have demonstrated a phosphorylation-dephosphorylation sequence in the suspensions. The results indicate that these reactions take place in vivo and may be physiologically relevant. Extraction of a protein kinase activity from rod outer segment membranes renders the membranes incapable of phosphorylation, but the light-activated reaction can be reconstituted by mixing the soluble extract and the \"depleted\" membranes. Illumination of the extract is without effect. Thus the light activation mechanism resides in the membranes. Regeneration of rhodopsin from opsin and 11-cis retinal does not influence the phosphorylation. Once activated, the reaction may use either rhodopsin or opsin as the substrate. Furthermore, 11-cis retinal regenerates rhodopsin from phosphorylated opsin without releasing bound phosphate. The isolated rod outer segment which contains regenerated rhodopsin thus differs from one that is dark adapted in that phosphate can remain bound and the phosphorylation reaction remains activated. Dark adaptation in vivo must include at least two membrane associated reactions beyond regeneration of rhodopsin's spectral properties: dephosphorylation, and the inactivation of the phosphorylation process."}
{"id": "PMID:1079807", "title": "Ultrastructural characteristics of the non-immune rosette-forming cell.", "content": "Non-immune rosette formation, where a non-sensitized lymphocyte is surrounded by three or more sheep red blood cells, is an in vitro technique which is thought to be specific in the identification of thymus-dependent (T) lymphocytes. Using the electron microscope and tissue culture techniques, we have studied the ultrastructure of the rosette-forming lymphocyte which has been stimulated by various cellular mitogens. Non-sensitized but stimulated lymphocytes, which form rosettes, have a morphology similar to that of previously identified T cells, adding further credence to the concept that non-sensitized rosette-forming cells represent a population of T cells. Changes in the binding pattern of the sheep red cell membrane to the lymphocyte have been identified which may represent an early phase of cell 'activation'. This marker of activation offers a potential method of studying membrane responses in attempts to localize cellular defects involving the T lymphocyte. In addition, preliminary studies using fluorescein-labelled sheep red blood cell fragments suggest specific attachment of labelled membrane to the T lymphocyte, allowing rapid identification of T cells in smears or tissue preparations.", "contents": "Ultrastructural characteristics of the non-immune rosette-forming cell. Non-immune rosette formation, where a non-sensitized lymphocyte is surrounded by three or more sheep red blood cells, is an in vitro technique which is thought to be specific in the identification of thymus-dependent (T) lymphocytes. Using the electron microscope and tissue culture techniques, we have studied the ultrastructure of the rosette-forming lymphocyte which has been stimulated by various cellular mitogens. Non-sensitized but stimulated lymphocytes, which form rosettes, have a morphology similar to that of previously identified T cells, adding further credence to the concept that non-sensitized rosette-forming cells represent a population of T cells. Changes in the binding pattern of the sheep red cell membrane to the lymphocyte have been identified which may represent an early phase of cell 'activation'. This marker of activation offers a potential method of studying membrane responses in attempts to localize cellular defects involving the T lymphocyte. In addition, preliminary studies using fluorescein-labelled sheep red blood cell fragments suggest specific attachment of labelled membrane to the T lymphocyte, allowing rapid identification of T cells in smears or tissue preparations."}
{"id": "PMID:1079808", "title": "Human bone marrow lymphocytes.  I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals.", "content": "This study was undertaken to determine the proportions and in vitro immune capacities of lymphocyte populations in the bone marrows of normal humans. Relatively pure mononuclear cell suspensions were obtained from bone marrow aspirates by linear sucrose gradient centrifugations. Simultaneous peripheral blood and bone marrow specimens from each individual were assayed for lymphocyte surface markers and mitogen responsiveness. Maximal possible contamination of bone marrow aspirates by peripheral blood was determined by performing aspirates on individuals who had received 51chromium-labeled autologous erythrocytes. Rhymus-derived (T) lymphocytes, as determined by the sheep red blood cell (E) rosette assay, comprised 8.6-(plus or minus 1.6)% of the total bone marrow lymphocyte pool. Bone marrow-derived (B) lymphocytes, as determined by the presence of a complement receptor, made up 15.4-(plus or minus 1.9)% of the lymphocyte pool whereas 74.6 (plus or minus 2.4)% of mononuclear cells lacked easily detectable surface markers. These findings could not be explained by contamination with peripheral blood lymphocytes since contamination was corrected for in the calculations. Lymphocyte-enriched suspensions of bone marrow cells responded to stimulation with phytohemagglutinin, concanalin A, and particularly pokeweed mitogen. In vitro incubations of bone marrow and peripheral blood lymphocytes with tritiated thymidine followed by determinations of E and erythrocyte antibody complement (EAC) rosettes were performed. Simultaneous rosetteradioautographs demonstrated that the proliferative potential of bone marrow B lymphocytes was greater than peripheral blood B lymphocytes (P less than 0.01). On the other hand, the proliferative potential of bone marrow T lymphocytes was the same as that of peripheral blood T lymphocytes. These findings demonstrate that in addition to containing B lymphocytes the normal bone marrow contains a small fraction of T lymphocytes similar to the mature T lymphocyte pool found in the peripheral blood. These T cells most probably enter the bone marrow parenchyma as part of the normal recirculating lymphocyte pool.", "contents": "Human bone marrow lymphocytes.  I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals. This study was undertaken to determine the proportions and in vitro immune capacities of lymphocyte populations in the bone marrows of normal humans. Relatively pure mononuclear cell suspensions were obtained from bone marrow aspirates by linear sucrose gradient centrifugations. Simultaneous peripheral blood and bone marrow specimens from each individual were assayed for lymphocyte surface markers and mitogen responsiveness. Maximal possible contamination of bone marrow aspirates by peripheral blood was determined by performing aspirates on individuals who had received 51chromium-labeled autologous erythrocytes. Rhymus-derived (T) lymphocytes, as determined by the sheep red blood cell (E) rosette assay, comprised 8.6-(plus or minus 1.6)% of the total bone marrow lymphocyte pool. Bone marrow-derived (B) lymphocytes, as determined by the presence of a complement receptor, made up 15.4-(plus or minus 1.9)% of the lymphocyte pool whereas 74.6 (plus or minus 2.4)% of mononuclear cells lacked easily detectable surface markers. These findings could not be explained by contamination with peripheral blood lymphocytes since contamination was corrected for in the calculations. Lymphocyte-enriched suspensions of bone marrow cells responded to stimulation with phytohemagglutinin, concanalin A, and particularly pokeweed mitogen. In vitro incubations of bone marrow and peripheral blood lymphocytes with tritiated thymidine followed by determinations of E and erythrocyte antibody complement (EAC) rosettes were performed. Simultaneous rosetteradioautographs demonstrated that the proliferative potential of bone marrow B lymphocytes was greater than peripheral blood B lymphocytes (P less than 0.01). On the other hand, the proliferative potential of bone marrow T lymphocytes was the same as that of peripheral blood T lymphocytes. These findings demonstrate that in addition to containing B lymphocytes the normal bone marrow contains a small fraction of T lymphocytes similar to the mature T lymphocyte pool found in the peripheral blood. These T cells most probably enter the bone marrow parenchyma as part of the normal recirculating lymphocyte pool."}
{"id": "PMID:1079810", "title": "Increased affinity of guinea pig thymocytes and thymus-dependent lymphocytes for papain-treated rabbit erythrocytes compared to untreated erythrocytes.", "content": "The affinity of guinea pig thymocytes and peripheral T lymphocytes for rabbit erythrocytes was found to be enhanced following treatment of the erythrocytes with papain. By increasing the numbers of rosette-forming cells and giving stronger, more stable rosettes, this procedure increases the usefulness of the reaction as a T cell marker in guinea pigs.", "contents": "Increased affinity of guinea pig thymocytes and thymus-dependent lymphocytes for papain-treated rabbit erythrocytes compared to untreated erythrocytes. The affinity of guinea pig thymocytes and peripheral T lymphocytes for rabbit erythrocytes was found to be enhanced following treatment of the erythrocytes with papain. By increasing the numbers of rosette-forming cells and giving stronger, more stable rosettes, this procedure increases the usefulness of the reaction as a T cell marker in guinea pigs."}
{"id": "PMID:1079847", "title": "Growth properties and alloantigenic expression of murine lymphoblastoid cell lines.", "content": "Murine lymphoblastoid cell lines were evaluated for their expression of Thy-1 and thymus leukemia (TL) differentiation alloantigens.  Two culture conditions were shown to affect this expression.  Cells grown in fetal bovine serum (FBS)-enriched medium expressed up to 15 times the amount of TL as cells grown in horse serum (HS)-enriched medium.  Thy-1 expression was less affected by the type of serum used for culture.  The phase of growth when the cells were harvested, was demonstrated to affect the expression of Thy-1.  The expression of Thy-1.2 for one cell line examined, L-251A, during logarithmic growth was threefold greater than cells collected during either lag or stationary growth.  When culture conditions were standardized a ranking of the amount of Thy-1 and TL expressed by several cell lines was made.  All cell lines, except one, L-1210, expressed Thy-1.  There was a 450-fold difference in the expression of Thy-1 between the cell lines evaluated.  Seven cell lines expressed TL-1,2,3 with a ninefold difference in the amount of expression.  The L-251A cell line was cultured in a 14 liter fermentor for a 26 day period.  During this time TL and Thy-1 expression did not vary significantly, demonstrating that lymphoblastoid cell lines can be cultured on a continuous basis and will continue to express their surface alloantigens.", "contents": "Growth properties and alloantigenic expression of murine lymphoblastoid cell lines. Murine lymphoblastoid cell lines were evaluated for their expression of Thy-1 and thymus leukemia (TL) differentiation alloantigens.  Two culture conditions were shown to affect this expression.  Cells grown in fetal bovine serum (FBS)-enriched medium expressed up to 15 times the amount of TL as cells grown in horse serum (HS)-enriched medium.  Thy-1 expression was less affected by the type of serum used for culture.  The phase of growth when the cells were harvested, was demonstrated to affect the expression of Thy-1.  The expression of Thy-1.2 for one cell line examined, L-251A, during logarithmic growth was threefold greater than cells collected during either lag or stationary growth.  When culture conditions were standardized a ranking of the amount of Thy-1 and TL expressed by several cell lines was made.  All cell lines, except one, L-1210, expressed Thy-1.  There was a 450-fold difference in the expression of Thy-1 between the cell lines evaluated.  Seven cell lines expressed TL-1,2,3 with a ninefold difference in the amount of expression.  The L-251A cell line was cultured in a 14 liter fermentor for a 26 day period.  During this time TL and Thy-1 expression did not vary significantly, demonstrating that lymphoblastoid cell lines can be cultured on a continuous basis and will continue to express their surface alloantigens."}
{"id": "PMID:1079848", "title": "On the role of the H-2 histocompatibility complex in determining the specificity of cytotoxic effector cells sensitized against syngeneic trinitrophenyl-modified targets.", "content": "Spleen cells cultured with syngeneic trinitrophenyl (TNP)-modified stimulator cells display a cytotoxic effect against syngeneic TNP-modified targets, but not against modified targets from unrelated H-2 haplotypes.  Targets that share the K and I region of the H-2 complex with the stimulator (or effector) cell are lysed to the same extent as the specific targets, while targets that share the I region only are not.  When only the D region is shared, a weak cytotoxic effect is observed.  Therefore, the stimulator (or effector) and target cell must share the K or D but not the I region of the H-2 complex in order for optimal cytotoxicity to occur.  Spleen cells sensitized to irradiated TNP-modified H-2-allogeneic cells are cytotoxic to these specific cells.  Coculture of F1 hybrid cells with irradiated TNP-modified parental cells result in a cytotoxic effect against only those specific parental cells and not TNP-modified cells from the other parent.  The cytotoxic effect of the F1 effector cells in the cell-mediated lympholysis test is blocked by the addition of unlabeled TNP-modified targets that are H-2 syngeneic with the sensitizing parental strain, but not H-2 syngeneic with the other parental strain.  These data demonstrate that the specificity of the effector cell in this syngeneic cytotoxicity system is directed against altered self H-2-controlled-gene products, rather than a requirement for sharing of histocompatibility genes between effector and target cell in order for lysis to occur.  The role of H-2 antigens in determining the sensitivity of a target cell to T-cell-mediated lysis is discussed.", "contents": "On the role of the H-2 histocompatibility complex in determining the specificity of cytotoxic effector cells sensitized against syngeneic trinitrophenyl-modified targets. Spleen cells cultured with syngeneic trinitrophenyl (TNP)-modified stimulator cells display a cytotoxic effect against syngeneic TNP-modified targets, but not against modified targets from unrelated H-2 haplotypes.  Targets that share the K and I region of the H-2 complex with the stimulator (or effector) cell are lysed to the same extent as the specific targets, while targets that share the I region only are not.  When only the D region is shared, a weak cytotoxic effect is observed.  Therefore, the stimulator (or effector) and target cell must share the K or D but not the I region of the H-2 complex in order for optimal cytotoxicity to occur.  Spleen cells sensitized to irradiated TNP-modified H-2-allogeneic cells are cytotoxic to these specific cells.  Coculture of F1 hybrid cells with irradiated TNP-modified parental cells result in a cytotoxic effect against only those specific parental cells and not TNP-modified cells from the other parent.  The cytotoxic effect of the F1 effector cells in the cell-mediated lympholysis test is blocked by the addition of unlabeled TNP-modified targets that are H-2 syngeneic with the sensitizing parental strain, but not H-2 syngeneic with the other parental strain.  These data demonstrate that the specificity of the effector cell in this syngeneic cytotoxicity system is directed against altered self H-2-controlled-gene products, rather than a requirement for sharing of histocompatibility genes between effector and target cell in order for lysis to occur.  The role of H-2 antigens in determining the sensitivity of a target cell to T-cell-mediated lysis is discussed."}
{"id": "PMID:1079849", "title": "The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens.", "content": "Helper cell induction to nonparticle antigens in vitro requires the cooperation of T cells and macrophages, but does not occur if the macrophages are allogenic.  The reasons for this were investigated.  Malfunction of allogenic macrophages was excluded by cultures with their syngenic T cells; suppressor cell induction was excluded by admixture experiments.  Thus, T cells and macrophages only cooperated if they were genetically similar.  The genetic locus (loci) involved was mapped.  Using congenic lines differing only at the H-2 complex, the genetic control of T-macrophage interaction was localized in the H-2 region.  Mice with intra H-2 recombinants were used to map the T-macrophage interaction locus in the I-A region of the H-2 complex (formerly known as poly-D, L-ala-poly-L-lys.  Recombinants were also used to exclude the presence of another T-macrophage locus either the K, I-B, or I-C, SS-Slp, or D regions of the H-2 complex.  Genetic restrictions for T-macrophage interaction in helper cell induction was shown in mice of the H-2-k, d, b, q, s genotypes as well as in H-2 recombinants.  The possible mechanisms and significance of this genetic restriction are discussed.", "contents": "The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens. Helper cell induction to nonparticle antigens in vitro requires the cooperation of T cells and macrophages, but does not occur if the macrophages are allogenic.  The reasons for this were investigated.  Malfunction of allogenic macrophages was excluded by cultures with their syngenic T cells; suppressor cell induction was excluded by admixture experiments.  Thus, T cells and macrophages only cooperated if they were genetically similar.  The genetic locus (loci) involved was mapped.  Using congenic lines differing only at the H-2 complex, the genetic control of T-macrophage interaction was localized in the H-2 region.  Mice with intra H-2 recombinants were used to map the T-macrophage interaction locus in the I-A region of the H-2 complex (formerly known as poly-D, L-ala-poly-L-lys.  Recombinants were also used to exclude the presence of another T-macrophage locus either the K, I-B, or I-C, SS-Slp, or D regions of the H-2 complex.  Genetic restrictions for T-macrophage interaction in helper cell induction was shown in mice of the H-2-k, d, b, q, s genotypes as well as in H-2 recombinants.  The possible mechanisms and significance of this genetic restriction are discussed."}
{"id": "PMID:1079850", "title": "Suppression of in vitro antibody synthesis by immunoglobulin-binding factor.", "content": "Alloantigen-activated mouse T cells secrete a factor which binds to the Fc fragment of IgG and blocks complement (C) activation by IgG (immunoglobulin-binding factor, IBF).  IBF was found to suppress the direct plaque-forming cell (PFC) response of mouse spleen cell cultures to sheep erythrocytes and to dinitrophenylated aminoethyldextran (T-independent antigen).  Purification of IBF by affinity chromatography on IgG-coated Sepharose columns led to an increase of the suppressive capacity with IgG, IgM, or Fab2 from IgG) the factor responsible for inhibiting the PFC response could not be dissociated from that responsible for the inhibitory activity of IBF on C-dependent hemolysis.  No effect was seen when cultures were pretreated for 6 h, or when IBF was added at 72 h.  These data are compatible with the view that IBF is a soluable mediator of suppressor T cells which may interfere with terminal differentiation of antibody-forming cell precursors.", "contents": "Suppression of in vitro antibody synthesis by immunoglobulin-binding factor. Alloantigen-activated mouse T cells secrete a factor which binds to the Fc fragment of IgG and blocks complement (C) activation by IgG (immunoglobulin-binding factor, IBF).  IBF was found to suppress the direct plaque-forming cell (PFC) response of mouse spleen cell cultures to sheep erythrocytes and to dinitrophenylated aminoethyldextran (T-independent antigen).  Purification of IBF by affinity chromatography on IgG-coated Sepharose columns led to an increase of the suppressive capacity with IgG, IgM, or Fab2 from IgG) the factor responsible for inhibiting the PFC response could not be dissociated from that responsible for the inhibitory activity of IBF on C-dependent hemolysis.  No effect was seen when cultures were pretreated for 6 h, or when IBF was added at 72 h.  These data are compatible with the view that IBF is a soluable mediator of suppressor T cells which may interfere with terminal differentiation of antibody-forming cell precursors."}
{"id": "PMID:1079851", "title": "Feedback induction of suppressor T-cell activity.", "content": "Adoptively transferred carrier immune T cells interact with nonimmune T cells in recipients in a fashion which generates specific immunosuppression although both the immune and normal cells function quite well as helper cells when not admixed.", "contents": "Feedback induction of suppressor T-cell activity. Adoptively transferred carrier immune T cells interact with nonimmune T cells in recipients in a fashion which generates specific immunosuppression although both the immune and normal cells function quite well as helper cells when not admixed."}
{"id": "PMID:1079852", "title": "Transplantation behavior of A.TH and A.TL T-cell lymphomas in congenic resistant and hybrid strains.", "content": "Seven spontaneously arising T-cell lymphomas originating in A.TH or A.TL mice, which are congenic for the immune response gene (I) chromosomal segment were described.  When transplanted into partner strains which were incompatible at the I region, the tumors were rapidly rejected.  Rejection was proposed to be due to the presence of antigens controlled by I-region genes.", "contents": "Transplantation behavior of A.TH and A.TL T-cell lymphomas in congenic resistant and hybrid strains. Seven spontaneously arising T-cell lymphomas originating in A.TH or A.TL mice, which are congenic for the immune response gene (I) chromosomal segment were described.  When transplanted into partner strains which were incompatible at the I region, the tumors were rapidly rejected.  Rejection was proposed to be due to the presence of antigens controlled by I-region genes."}
{"id": "PMID:1079853", "title": "IgM-induced tumor cell cytotoxicity mediated by normal thymocytes.", "content": "In summary, we have found that IgM, from mice which have undergone regression of primary MSV tumors, will induce cytotoxicity against the appropriate target cells by normal splenocytes and normal thymocytes.  The thymocyte-induced cytotoxicity i", "contents": "IgM-induced tumor cell cytotoxicity mediated by normal thymocytes. In summary, we have found that IgM, from mice which have undergone regression of primary MSV tumors, will induce cytotoxicity against the appropriate target cells by normal splenocytes and normal thymocytes.  The thymocyte-induced cytotoxicity i"}
{"id": "PMID:1079854", "title": "Cell migration into the \"established\" lateral motor column in Rana pipiens larvae.", "content": "Neuronal turnover has been demonstrated during the period of differentiation and maturationof motor neurons in the developing lateral motor columns of Rana pipiens larvae. As determined by thymidine-3H labeling, cells continued to migrate into the lateral motor column (LMC)while there was a concurrent reduction in LMC cell numbers. Notably, cells in the lumbosacral LMC ceased to be labeled following thymidine-3H injection at an earlier time than forthe brachial LMC. This is consistent with a caudo-cephalic direction of development amongthe two pairs of motor columns in the spinal cord. Since neuronal turnover occurs followingthe initial formation of the LMC and continues after the maximum number of cells has beenattained, it is proposed that neuronal turnover may be a mechansim that allows for neuralreadjustment and refinement during spinal cord development.", "contents": "Cell migration into the \"established\" lateral motor column in Rana pipiens larvae. Neuronal turnover has been demonstrated during the period of differentiation and maturationof motor neurons in the developing lateral motor columns of Rana pipiens larvae. As determined by thymidine-3H labeling, cells continued to migrate into the lateral motor column (LMC)while there was a concurrent reduction in LMC cell numbers. Notably, cells in the lumbosacral LMC ceased to be labeled following thymidine-3H injection at an earlier time than forthe brachial LMC. This is consistent with a caudo-cephalic direction of development amongthe two pairs of motor columns in the spinal cord. Since neuronal turnover occurs followingthe initial formation of the LMC and continues after the maximum number of cells has beenattained, it is proposed that neuronal turnover may be a mechansim that allows for neuralreadjustment and refinement during spinal cord development."}
{"id": "PMID:1079855", "title": "Prolactin-thyroxine antagonism and the metamorphosis of visual pigments in Rana catesbeiana tadpoles.", "content": "The relationship between prolactin-thyroxin antagonism and the metamorphosis of visual pigments in larval amphibians has been investigated using bullfrog (Rana catesbeiana) tadpoles. Althought prolactin-thyroxine antagonism is demonstrable by morphological criteria, ovineprolactin does not appear to anatagonize thyroxine-induced rhodopsin synthesis. The hypothesisis offered that prolactin-thyroxine antagonism is the result of differential gene activities which are opposite in their physiological effects.", "contents": "Prolactin-thyroxine antagonism and the metamorphosis of visual pigments in Rana catesbeiana tadpoles. The relationship between prolactin-thyroxin antagonism and the metamorphosis of visual pigments in larval amphibians has been investigated using bullfrog (Rana catesbeiana) tadpoles. Althought prolactin-thyroxine antagonism is demonstrable by morphological criteria, ovineprolactin does not appear to anatagonize thyroxine-induced rhodopsin synthesis. The hypothesisis offered that prolactin-thyroxine antagonism is the result of differential gene activities which are opposite in their physiological effects."}
{"id": "PMID:1079856", "title": "The effect of prolactin and thyroxine on tail resorption in R. pipiens: in vivo and in vitro.", "content": "Thyroxine induces tail resorption in anurans both in vivo and in vitro. This event is accompanied by an increase in the activity of the hydrolytic enzymes acid phosphtase and B-N-acetylglucosaminidase. Prolactin treatment promotes tail growth and directly antagonizes the action of thyroxine on tail tissues. This is evident at the gross morphological level as well as at the biochemical level where prolactin inhibits the increase in activity on the hydrolytic enzymes that occurs during thyroid hormone induced resorption.", "contents": "The effect of prolactin and thyroxine on tail resorption in R. pipiens: in vivo and in vitro. Thyroxine induces tail resorption in anurans both in vivo and in vitro. This event is accompanied by an increase in the activity of the hydrolytic enzymes acid phosphtase and B-N-acetylglucosaminidase. Prolactin treatment promotes tail growth and directly antagonizes the action of thyroxine on tail tissues. This is evident at the gross morphological level as well as at the biochemical level where prolactin inhibits the increase in activity on the hydrolytic enzymes that occurs during thyroid hormone induced resorption."}
{"id": "PMID:1079858", "title": "The formation of virus polyribosomes in L cells infected with vaccinia virus.", "content": "The fate of early virus messenger RNA in the cytoplasm of vaccinia-infected L cells has been studied during the first hour after infection. The RNA is made in the virus core structure from which it is rapidly released. It accumulates in the polyribsome fraction, where at least 75% is bound to ribosomes through an EDTA-sensitive link. Three distinct structures have been identified as possible intermediates in virus polyribosome formation. The first is a ribonucleoprotein complex (RNP) in which virus RNA is associated with cellular proteins. A complex having apparently similar properties, is formed when virus RNA is added to a cytoplasmic extract in vitro. The other two structures may consist of an RNP moiety associated with the small ribosomal subunit, or with a single ribosome. At least part of the RNA isolated as RNP appears to be a precursor of the virus messenger found in polyribosomes.", "contents": "The formation of virus polyribosomes in L cells infected with vaccinia virus. The fate of early virus messenger RNA in the cytoplasm of vaccinia-infected L cells has been studied during the first hour after infection. The RNA is made in the virus core structure from which it is rapidly released. It accumulates in the polyribsome fraction, where at least 75% is bound to ribosomes through an EDTA-sensitive link. Three distinct structures have been identified as possible intermediates in virus polyribosome formation. The first is a ribonucleoprotein complex (RNP) in which virus RNA is associated with cellular proteins. A complex having apparently similar properties, is formed when virus RNA is added to a cytoplasmic extract in vitro. The other two structures may consist of an RNP moiety associated with the small ribosomal subunit, or with a single ribosome. At least part of the RNA isolated as RNP appears to be a precursor of the virus messenger found in polyribosomes."}
{"id": "PMID:1079859", "title": "Isotope cisternography and ventriculography in congenital anomalies of the central nervous system.", "content": "The author discusses the value of isotope cisternography and ventriculography in the diagnosis of a variety of congenital anomalies of the central nervous system (CNS) associated with either hydrocephalus or a disturbance in cerebrospinal fluid (CSF) flow and absorption. In sincipital encephaloceles the techniques demonstrate CSF communication with the anomalous sac and the frequently associated hydrocephalus with occipital lesions. In porencephaly and cerebral agenesis, the isotope demonstrates ventricular reflux and remains in the cystic dilatation indicating abnormal CSF absorption; isotope ventriculography also reveals a delayed CSF absorption and the possible sites of CSF obstruction in Arnold-Chiari malformations and Dandy-Walker syndrome. In addition to their differential diagnostic value isotope studies can be a helpful guide in the choice of the proper surgical treatment of some CNS anomalies.", "contents": "Isotope cisternography and ventriculography in congenital anomalies of the central nervous system. The author discusses the value of isotope cisternography and ventriculography in the diagnosis of a variety of congenital anomalies of the central nervous system (CNS) associated with either hydrocephalus or a disturbance in cerebrospinal fluid (CSF) flow and absorption. In sincipital encephaloceles the techniques demonstrate CSF communication with the anomalous sac and the frequently associated hydrocephalus with occipital lesions. In porencephaly and cerebral agenesis, the isotope demonstrates ventricular reflux and remains in the cystic dilatation indicating abnormal CSF absorption; isotope ventriculography also reveals a delayed CSF absorption and the possible sites of CSF obstruction in Arnold-Chiari malformations and Dandy-Walker syndrome. In addition to their differential diagnostic value isotope studies can be a helpful guide in the choice of the proper surgical treatment of some CNS anomalies."}
{"id": "PMID:1079860", "title": "Neuroepithelial (colloid) cysts of the septum pellucidum.", "content": "The authors present two cases of neuroepithelial (colloid) cysts found above the diencephalic roof, occupying the space between the two fornices and the two leaves of the septum pellucidum, and describe the clinical history, neurological, and neuroradiological findings. Both lesions were removed through a right transventricular-transseptal approach. No other report of such a lesion in this location could be found in the review of literature. The pathogenesis of these cysts, both above and below the diencephalic roof, is discussed in light of the developmental anatomy of the area under consideration. In view of their origin from the neuroepithelium in the diencephalic roof, whether by a process of invagination into the third ventricle or by evagination into the velum interpositum, the so-called colloid cysts are more appropriately termed neuroepithelial cysts.", "contents": "Neuroepithelial (colloid) cysts of the septum pellucidum. The authors present two cases of neuroepithelial (colloid) cysts found above the diencephalic roof, occupying the space between the two fornices and the two leaves of the septum pellucidum, and describe the clinical history, neurological, and neuroradiological findings. Both lesions were removed through a right transventricular-transseptal approach. No other report of such a lesion in this location could be found in the review of literature. The pathogenesis of these cysts, both above and below the diencephalic roof, is discussed in light of the developmental anatomy of the area under consideration. In view of their origin from the neuroepithelium in the diencephalic roof, whether by a process of invagination into the third ventricle or by evagination into the velum interpositum, the so-called colloid cysts are more appropriately termed neuroepithelial cysts."}
{"id": "PMID:1079862", "title": "Effects of massive doses of ergocalciferol plus cholesterol on pregnant rats and their offspring.", "content": "Ergocalciferol (320,000 or 480,000 IU/kg) plus cholesterol (60 mg/kg) in olive oil solution was administered daily on 1, 2, or 4 consecutive days to pregnant rats from 9,10, 14, or 18 of gestation.  The control animals received only olive oil.  Disseminated lesions of metastic calcinosis were found in various tissues, in the coronary arteries and myocardium, in the media of the abnormal aorta, in the lung and pleura, in the gastoinstestinal tract, and in the kidney.  This is in contrast to the atherosclerosis described in nonpregnant rats fed a similiar diet.  A significant decline in maternal weight as well as a high rate of morbidity and mortality was observed.  In mothers killed on day 22 of pregnancy, fetal and placental growths appeared significantly retarded suggesting a direct effect of the steroid or its more active metabolite, 1,25-dihydroxycholecalciferol, on the fetus or the trophoblastic tissue.  Fetal bone lesionsassociated with a generalized retardation of ossification, placental edema, or calcification accompanied by a loss of the normal structure of the placenta and degenerative manifestation at this level were observed.  Moreover, we noted a striking alteration of the fetal face in 33-39% of experimental fetuses, called by us carnival fetuses.", "contents": "Effects of massive doses of ergocalciferol plus cholesterol on pregnant rats and their offspring. Ergocalciferol (320,000 or 480,000 IU/kg) plus cholesterol (60 mg/kg) in olive oil solution was administered daily on 1, 2, or 4 consecutive days to pregnant rats from 9,10, 14, or 18 of gestation.  The control animals received only olive oil.  Disseminated lesions of metastic calcinosis were found in various tissues, in the coronary arteries and myocardium, in the media of the abnormal aorta, in the lung and pleura, in the gastoinstestinal tract, and in the kidney.  This is in contrast to the atherosclerosis described in nonpregnant rats fed a similiar diet.  A significant decline in maternal weight as well as a high rate of morbidity and mortality was observed.  In mothers killed on day 22 of pregnancy, fetal and placental growths appeared significantly retarded suggesting a direct effect of the steroid or its more active metabolite, 1,25-dihydroxycholecalciferol, on the fetus or the trophoblastic tissue.  Fetal bone lesionsassociated with a generalized retardation of ossification, placental edema, or calcification accompanied by a loss of the normal structure of the placenta and degenerative manifestation at this level were observed.  Moreover, we noted a striking alteration of the fetal face in 33-39% of experimental fetuses, called by us carnival fetuses."}
{"id": "PMID:1079869", "title": "Modification of sodium channel gating in frog myelinated nerve fibres by Centruroides sculpturatus scorpion venom.", "content": "1. The effect of Centruroides sculpturatus scorpion venom on single frog myelinated nerve fibres was studied. Sodium currents through the nodal membrane were measured under voltage-clamp conditions before and after exposure to venom in Ringer solution 1-5 mug/ml. for 1-3 min. 2. Centruroides venom brings about repetitive firing and increased membrane potential noise. Spontaneous firing was also observed. Eventually the nodal membrane becomes inexcitable following venom treatment. 3. Under voltage clamp with a step depolarization of the membrane potential, activation and inactivation of sodium currents turns on, reaches a peak within about 25 msec, and then declines over several hundred milliseconds. As the amplitude and duration of the depolarizing pulse are increased, the size of the venom-induced current that follows also increases. 4. The venom-induced current turns on exponentially with a time constant near the value of the time constant for recovery from inactivation, tau-h, at the resting membrane potential. A depolarizing pulse inactivates this new current component, while a hyperpolarizing pulse leads to a larger venom-induced current immediately after the hyperpolarization. Its time course and membrane potential dependence indicate that the venom-induced current is modulated by the sodium inactivation process. 5. The membrane potential dependence of sodium activation in some channels is shifted by 40-50 mV in the hyperpolarizing direction. Depolarization increases the proportion of channels with shifted activation gating by first-older kinetics. Following a depolarizing pulse the activation parameter, m-3, remains elevated for hundreds of milliseconds, allowing channels to reopen as recovery from inactivation occurs. 6. A kinetic model with normal inactivation gating and shifted activation gating in some channels accounts for the observed voltage-clamp currents and for the repetitive firing evoked by Centruroides venom. In the model normal channels are converted to channels with shifted activation gating by a voltage dependent reaction. 7. The results suggest limits to possible coupling between sodium channel activation and inactivation. Transitions of the inactivation parameter, h, can occur normally in channels with a shifted membrane potential dependence for activation.", "contents": "Modification of sodium channel gating in frog myelinated nerve fibres by Centruroides sculpturatus scorpion venom. 1. The effect of Centruroides sculpturatus scorpion venom on single frog myelinated nerve fibres was studied. Sodium currents through the nodal membrane were measured under voltage-clamp conditions before and after exposure to venom in Ringer solution 1-5 mug/ml. for 1-3 min. 2. Centruroides venom brings about repetitive firing and increased membrane potential noise. Spontaneous firing was also observed. Eventually the nodal membrane becomes inexcitable following venom treatment. 3. Under voltage clamp with a step depolarization of the membrane potential, activation and inactivation of sodium currents turns on, reaches a peak within about 25 msec, and then declines over several hundred milliseconds. As the amplitude and duration of the depolarizing pulse are increased, the size of the venom-induced current that follows also increases. 4. The venom-induced current turns on exponentially with a time constant near the value of the time constant for recovery from inactivation, tau-h, at the resting membrane potential. A depolarizing pulse inactivates this new current component, while a hyperpolarizing pulse leads to a larger venom-induced current immediately after the hyperpolarization. Its time course and membrane potential dependence indicate that the venom-induced current is modulated by the sodium inactivation process. 5. The membrane potential dependence of sodium activation in some channels is shifted by 40-50 mV in the hyperpolarizing direction. Depolarization increases the proportion of channels with shifted activation gating by first-older kinetics. Following a depolarizing pulse the activation parameter, m-3, remains elevated for hundreds of milliseconds, allowing channels to reopen as recovery from inactivation occurs. 6. A kinetic model with normal inactivation gating and shifted activation gating in some channels accounts for the observed voltage-clamp currents and for the repetitive firing evoked by Centruroides venom. In the model normal channels are converted to channels with shifted activation gating by a voltage dependent reaction. 7. The results suggest limits to possible coupling between sodium channel activation and inactivation. Transitions of the inactivation parameter, h, can occur normally in channels with a shifted membrane potential dependence for activation."}
{"id": "PMID:1079871", "title": "Studies on convulsants in the isolated frog spinal cord. I. Antagonism of amino acid responses.", "content": "1. The isolated frog spinal cord was used to study the effects of picrotoxin, bicuculline, and strychnine on the responses of primary afferents to amino acids. Recording was by sucrose gap technique. 2. A series of neutral amino acids was found to depolarize primary afferents. Optimal activity was obtained by an amino acid whose carboxyl and amino groups were separated by a three-carbon chain length (i.e. GABA). Amino acids with shorter (i.e. beta-alanine, glycine) or longer (i.e. delta-aminovaleric acid, epsilon-aminocaproic acid) distances between the charged groups were less potent. Imidazoleacetic acid was the most potent depolarizing agent tested. 3. Picrotoxin and bicuculline antagonized the primary afferent depolarizations of a number of amino acids tested with equal specificity. Depolarizing responses to standard (10- minus 3 M) concentrations of beta-alanine and taurine were completely blocked by these convulsants, while depolarizations to 10- minus 3 gamma-aminobutyric acid (GABA) were only partially antagonized. Glycine responses were unaffected by these agentsk; Strychnine completely blocked beta-alanine and taurine depolarizations and incompletely antagonized several other neutral amino acids. GABA, glutamate, and glycine depolarizations were not affected. 5. These results suggest that there are at least three distinct populations of neutral amino acid receptors on primary afferent terminals: a GABA-like receptor, a taurine/beta-alanine receptor, and a glycine-like receptor. The strychnine resistance of the glycine responses indictaes that the primary afferent receptors for glycine differ from those on the somata of spinal neurones.", "contents": "Studies on convulsants in the isolated frog spinal cord. I. Antagonism of amino acid responses. 1. The isolated frog spinal cord was used to study the effects of picrotoxin, bicuculline, and strychnine on the responses of primary afferents to amino acids. Recording was by sucrose gap technique. 2. A series of neutral amino acids was found to depolarize primary afferents. Optimal activity was obtained by an amino acid whose carboxyl and amino groups were separated by a three-carbon chain length (i.e. GABA). Amino acids with shorter (i.e. beta-alanine, glycine) or longer (i.e. delta-aminovaleric acid, epsilon-aminocaproic acid) distances between the charged groups were less potent. Imidazoleacetic acid was the most potent depolarizing agent tested. 3. Picrotoxin and bicuculline antagonized the primary afferent depolarizations of a number of amino acids tested with equal specificity. Depolarizing responses to standard (10- minus 3 M) concentrations of beta-alanine and taurine were completely blocked by these convulsants, while depolarizations to 10- minus 3 gamma-aminobutyric acid (GABA) were only partially antagonized. Glycine responses were unaffected by these agentsk; Strychnine completely blocked beta-alanine and taurine depolarizations and incompletely antagonized several other neutral amino acids. GABA, glutamate, and glycine depolarizations were not affected. 5. These results suggest that there are at least three distinct populations of neutral amino acid receptors on primary afferent terminals: a GABA-like receptor, a taurine/beta-alanine receptor, and a glycine-like receptor. The strychnine resistance of the glycine responses indictaes that the primary afferent receptors for glycine differ from those on the somata of spinal neurones."}
{"id": "PMID:1079873", "title": "The interaction of lithium ions with the sodium-potassium pump in frog skeletal muscle.", "content": "1. The effects of external Li on Na and K efflux as well as those on K influx were studied in high Na muscles from Rana pipiens. 2. In the absence of external Ba, substitution of K-free Li for K-free Mg resulted in an increase of both Na and K efflux. The additional of ouabain produced an inhibition of Na efflux and at the same time a marked increase in the efflux of K. 3. K permeability was greatly reduced by adding 2 mM-Ba to the incubation solutions. Under these conditions, Li gave rise to a ouabain sensitive Na efflux which was 57% of that in the absence of Ba. On the other hand, the efflux of K was only slightly increased and was not affected further by ouabain. 4. The activation curves of Na efflux against the stimulating cation concentration in Na-free Mg-Ba Ringer followed a more or less hyperbolic function for both K and Li. While half-maximal activation was attained at higher concentrations of Li than of K, the maximal efflux in Li was smaller than in K. 5. The extra Na efflux produced by K was increased when Li was added to the media. This increment was not a simple additive effect and was independent of the Li concentration. In addition, at some concentrations Li increased the ouabain-sensitive K influx, whereas at others it reduced it. 6. Reversible changes in membrane permeability to monovalent cations were accomplished by incubating the muscles in the presence of Nystatin, 50 mug/ml. When internal K was reduced to values around 1-2 mumole/g (using Li as a replacement), thus minimizing the possibility of K leaking out of the cells, both Ko and Lio were able to promote a ouabain-sensitive extra efflux of Na. 7. The residual Na efflux in (K+Na)-free solutions was not affected by the removal of Ca from the media in either Mg or Li solutions, both in the absence and the presence of Ba. On the other hand, the values for the residual efflux were higher in Mg (0-00228 min-1) than in Li (0.00135 min-1). 8. These results fully support the notion that Li ions have a K-like activating action on the Na pump in muscles. In addition, they suggest that some other kind of interaction may exist between Li and the Na-K pump.", "contents": "The interaction of lithium ions with the sodium-potassium pump in frog skeletal muscle. 1. The effects of external Li on Na and K efflux as well as those on K influx were studied in high Na muscles from Rana pipiens. 2. In the absence of external Ba, substitution of K-free Li for K-free Mg resulted in an increase of both Na and K efflux. The additional of ouabain produced an inhibition of Na efflux and at the same time a marked increase in the efflux of K. 3. K permeability was greatly reduced by adding 2 mM-Ba to the incubation solutions. Under these conditions, Li gave rise to a ouabain sensitive Na efflux which was 57% of that in the absence of Ba. On the other hand, the efflux of K was only slightly increased and was not affected further by ouabain. 4. The activation curves of Na efflux against the stimulating cation concentration in Na-free Mg-Ba Ringer followed a more or less hyperbolic function for both K and Li. While half-maximal activation was attained at higher concentrations of Li than of K, the maximal efflux in Li was smaller than in K. 5. The extra Na efflux produced by K was increased when Li was added to the media. This increment was not a simple additive effect and was independent of the Li concentration. In addition, at some concentrations Li increased the ouabain-sensitive K influx, whereas at others it reduced it. 6. Reversible changes in membrane permeability to monovalent cations were accomplished by incubating the muscles in the presence of Nystatin, 50 mug/ml. When internal K was reduced to values around 1-2 mumole/g (using Li as a replacement), thus minimizing the possibility of K leaking out of the cells, both Ko and Lio were able to promote a ouabain-sensitive extra efflux of Na. 7. The residual Na efflux in (K+Na)-free solutions was not affected by the removal of Ca from the media in either Mg or Li solutions, both in the absence and the presence of Ba. On the other hand, the values for the residual efflux were higher in Mg (0-00228 min-1) than in Li (0.00135 min-1). 8. These results fully support the notion that Li ions have a K-like activating action on the Na pump in muscles. In addition, they suggest that some other kind of interaction may exist between Li and the Na-K pump."}
{"id": "PMID:1079876", "title": "Induction of L-forms of Haemophilus influenzae in vitro.", "content": "The induction of L-forms of Haemophilus influenzae by penicillin, amoxycillin and glycine has been studies in vitro on a nutrient-agar medium. The minimal inducing concentrations of the antibiotics were generally the same as their minimal inhibitory concentrations, but the addition of a sub-inducing concentration of glycine lowered the minimal inducing concentration of penicillin. Preliminary observations have shown that L-forms are induced by penicillin or amoxycillin on a medium in which mucoid sputum forms the sole source of nutrients, and that they remain viable for at least 48 h in the absence of added osmotic stabiliser. The minimal inducing concentration on \"sputum agar\" is within the range of concentrations measured in sputum from patients receiving amoxycillin therapy. The implications of these observations in relation to bactericidal therapy of haemophilus infections of the respiratory tract are discussed.", "contents": "Induction of L-forms of Haemophilus influenzae in vitro. The induction of L-forms of Haemophilus influenzae by penicillin, amoxycillin and glycine has been studies in vitro on a nutrient-agar medium. The minimal inducing concentrations of the antibiotics were generally the same as their minimal inhibitory concentrations, but the addition of a sub-inducing concentration of glycine lowered the minimal inducing concentration of penicillin. Preliminary observations have shown that L-forms are induced by penicillin or amoxycillin on a medium in which mucoid sputum forms the sole source of nutrients, and that they remain viable for at least 48 h in the absence of added osmotic stabiliser. The minimal inducing concentration on \"sputum agar\" is within the range of concentrations measured in sputum from patients receiving amoxycillin therapy. The implications of these observations in relation to bactericidal therapy of haemophilus infections of the respiratory tract are discussed."}
{"id": "PMID:1079877", "title": "Aldosterone-induced moulting in amphibian skin and its effect on electrical capacitance.", "content": "The resistance and capacitance of the isolated amphibian skin have been determined from measurements of the response of the voltage across the skin to small steps to current. Previous work indicates that the electrical impedance of frog skin, when the skin is bathed with Ringer's solution on both sides, is largely determined by the properties of the functional outward-facing membrane of the skin, the outer membrane of the stratum granulosum (P.G. Smith, 1971, Acta Physiol. Scand. 81:355). This membrane can be represented by a resistance and capacitance in parallel. Aldosterone, which induces conversion of the s. granulosum into a cornified cell layer and transformation of the cell layer below into a new s. granulosum, also causes a transient rise in resistance and a short-lived decrease in capacitance to about one-half its initial value. It is suggested that these electrical changes are caused by the transitory presence of two functional outward-facing membranes in series. The method of determining resistance and capacitance from the voltage response is discussed in the Appendix.", "contents": "Aldosterone-induced moulting in amphibian skin and its effect on electrical capacitance. The resistance and capacitance of the isolated amphibian skin have been determined from measurements of the response of the voltage across the skin to small steps to current. Previous work indicates that the electrical impedance of frog skin, when the skin is bathed with Ringer's solution on both sides, is largely determined by the properties of the functional outward-facing membrane of the skin, the outer membrane of the stratum granulosum (P.G. Smith, 1971, Acta Physiol. Scand. 81:355). This membrane can be represented by a resistance and capacitance in parallel. Aldosterone, which induces conversion of the s. granulosum into a cornified cell layer and transformation of the cell layer below into a new s. granulosum, also causes a transient rise in resistance and a short-lived decrease in capacitance to about one-half its initial value. It is suggested that these electrical changes are caused by the transitory presence of two functional outward-facing membranes in series. The method of determining resistance and capacitance from the voltage response is discussed in the Appendix."}
{"id": "PMID:1079878", "title": "Influx and efflux of sodium at the outer surface of frog skin.", "content": "The unidirectional Na influx j12, and Na efflux, j12, at the epithelial surface of the frog skin were determined under various experimental conditions. The j21 was taken as the difference between j12 and the simultaneously measured shortcircuit current (SCC). Errors in j12 determination originating from various transport rates within the skin were kept to a minimum using a normalization procedure. Under control conditions, j12 (1.20 muEquiv/cm-2. hr) was found to be only slightly larger than the SCC (1.10 MUEquiv/cm-2. hr). After inhibition of the transepithelial Na transport by amiloride, ouabain, low temperature and low Na concentration, the reduction of j12 and SCC was almost identical, indicating that the entrance of Na into the epithelium is rate limiting for the transepithelial transport. Compared to the control, j21 remained unchanged after amiloride and ouabain, but was insignificantly reduced at low temperature and significantly reduced at low Na concentration. These data are consistent with the assumption that the Na efflux follows mainly an extracellular pathway.", "contents": "Influx and efflux of sodium at the outer surface of frog skin. The unidirectional Na influx j12, and Na efflux, j12, at the epithelial surface of the frog skin were determined under various experimental conditions. The j21 was taken as the difference between j12 and the simultaneously measured shortcircuit current (SCC). Errors in j12 determination originating from various transport rates within the skin were kept to a minimum using a normalization procedure. Under control conditions, j12 (1.20 muEquiv/cm-2. hr) was found to be only slightly larger than the SCC (1.10 MUEquiv/cm-2. hr). After inhibition of the transepithelial Na transport by amiloride, ouabain, low temperature and low Na concentration, the reduction of j12 and SCC was almost identical, indicating that the entrance of Na into the epithelium is rate limiting for the transepithelial transport. Compared to the control, j21 remained unchanged after amiloride and ouabain, but was insignificantly reduced at low temperature and significantly reduced at low Na concentration. These data are consistent with the assumption that the Na efflux follows mainly an extracellular pathway."}
{"id": "PMID:1079880", "title": "Pulmonary emphysema and liver disease.  Occurrence in an alpha-antitrypsin-deficient adult.", "content": "An elderly woman manifested both pulmonary and hepatic disease secondary to alpha 1-antitrypsin deficiency.  The liver biopsy specimen showed the characteristic findings of this condition, most importantly, the presence of PAS-positive, diastase-resistant intracytoplasmic inclusions.", "contents": "Pulmonary emphysema and liver disease.  Occurrence in an alpha-antitrypsin-deficient adult. An elderly woman manifested both pulmonary and hepatic disease secondary to alpha 1-antitrypsin deficiency.  The liver biopsy specimen showed the characteristic findings of this condition, most importantly, the presence of PAS-positive, diastase-resistant intracytoplasmic inclusions."}
{"id": "PMID:1079889", "title": "Effects of tonicity on the resting tension in bullfrog ventricle.", "content": "A study of the effects of hyper -and hypotonic media on resting tension were examined in ventricular muscle of the bullfrog was undertaken because only few studies on resting tension from the viewpoint of E-C coupling in the heart muscle are available. Thin strips obtained from the bullfrog ventricle were perfused in the sucrose-gapped chamber, which allowed the effective stimulation and the simultaneous measurement of the contractile tension and the intracellular membrane potential. Hypertonic solutions up to 3.0 T, prepared by adding NaCl or sucrose, induced a triphasic response,  namely an initial rapid decline, a secondary slow development of contracture and a final transient off-contraction. Both the second and the final responses were strongly sensitive to the calcium concentration of the bathing medium and were completely abolished by depleting external calcium ions. The initial decreasing phase was not sensitive to external calcium but was progressively augmented by increasing the initial muscle length, whereas the dehydration process, estimated by measuring the muscle weight, was unaffected by muscle stretch. The changes in the membrane potential were not essential for these responses. It was concluded that the initial tension decline results from the passive process due to volume change while the sustained contracture as well as the transient off-contraction are produced by some active process involved in E-C coupling. Hypotonic solutions induced a monophasic contracture which is insensitive both to the external calcium concentration and muscle stretch. The possible mechanism for the genesis of the response was discussed.", "contents": "Effects of tonicity on the resting tension in bullfrog ventricle. A study of the effects of hyper -and hypotonic media on resting tension were examined in ventricular muscle of the bullfrog was undertaken because only few studies on resting tension from the viewpoint of E-C coupling in the heart muscle are available. Thin strips obtained from the bullfrog ventricle were perfused in the sucrose-gapped chamber, which allowed the effective stimulation and the simultaneous measurement of the contractile tension and the intracellular membrane potential. Hypertonic solutions up to 3.0 T, prepared by adding NaCl or sucrose, induced a triphasic response,  namely an initial rapid decline, a secondary slow development of contracture and a final transient off-contraction. Both the second and the final responses were strongly sensitive to the calcium concentration of the bathing medium and were completely abolished by depleting external calcium ions. The initial decreasing phase was not sensitive to external calcium but was progressively augmented by increasing the initial muscle length, whereas the dehydration process, estimated by measuring the muscle weight, was unaffected by muscle stretch. The changes in the membrane potential were not essential for these responses. It was concluded that the initial tension decline results from the passive process due to volume change while the sustained contracture as well as the transient off-contraction are produced by some active process involved in E-C coupling. Hypotonic solutions induced a monophasic contracture which is insensitive both to the external calcium concentration and muscle stretch. The possible mechanism for the genesis of the response was discussed."}
{"id": "PMID:1079890", "title": "[Calcinosis of the cardiac coronary arteries].", "content": "In patients who had died due to various diseases and also in practically healthy individuals aged from 10 to 79 years calcinosis of the coronary arteries was studied morphometrically and histologically. The incidence and the extend of calcinosis implicating the coronary arteries increased with growing age and were more pronounced in males than in females. In analysis of observations uncovered, irrespective of the cause responsible for death, a direct relationship between the area of calcinosis and that of atherosclerosis in grosso modo (correlation factor of 0.46) was noted; the diseased in consequence of diverse affections demonstrated considerable variations in the development of arterial calcinosis. More often than not calcinosis of the coronary arteries was definable in atherosclerotic plaques carrying an important fibrotic component, in cases of necrosis and in those marked by a low lipids content and poor vasculalarization of an altered wall of the coronary artery. Calcinosis of the coronary arteries occurred more often than did their stenosis on 50% or more of the lumen. There has been established a direct relation between the frequency of the coronary artery stenosis and the area of calcinosis. The latter usually appears as a sign of disseminated atherosclerosis rather than as that of a progressive atherosclerotic process.", "contents": "[Calcinosis of the cardiac coronary arteries]. In patients who had died due to various diseases and also in practically healthy individuals aged from 10 to 79 years calcinosis of the coronary arteries was studied morphometrically and histologically. The incidence and the extend of calcinosis implicating the coronary arteries increased with growing age and were more pronounced in males than in females. In analysis of observations uncovered, irrespective of the cause responsible for death, a direct relationship between the area of calcinosis and that of atherosclerosis in grosso modo (correlation factor of 0.46) was noted; the diseased in consequence of diverse affections demonstrated considerable variations in the development of arterial calcinosis. More often than not calcinosis of the coronary arteries was definable in atherosclerotic plaques carrying an important fibrotic component, in cases of necrosis and in those marked by a low lipids content and poor vasculalarization of an altered wall of the coronary artery. Calcinosis of the coronary arteries occurred more often than did their stenosis on 50% or more of the lumen. There has been established a direct relation between the frequency of the coronary artery stenosis and the area of calcinosis. The latter usually appears as a sign of disseminated atherosclerosis rather than as that of a progressive atherosclerotic process."}
{"id": "PMID:1079891", "title": "[Effect of trypsin-like enzyme inhibitor on certain metabolic processes in the acute period of experimental myocardial ischemia].", "content": "The effect of trasylol upon the activeness of proteolysis in the cytoplasm and mitochondrial-lysosomal fractions of the heart muscle was studied in the acute experimental period of myocardial ischaemia (5 days) along with a determination of the spectrum of the iso-enzymes of the lactate- and malate- dehydrogenases. For the evaluation of oxidation processes in vitro the iections. Trasylol was found to limit the proteolysis in the cytostructures of the myocardium cell, to increase the activity of the transaminases, as well as to help the normalization of thespectrum of the NAD-dependent iso-enzymes. At the same time, under the effect of the employed drug, the myocardial consumption of the radioactive carbon from the acetate Na-2C-14 increases.", "contents": "[Effect of trypsin-like enzyme inhibitor on certain metabolic processes in the acute period of experimental myocardial ischemia]. The effect of trasylol upon the activeness of proteolysis in the cytoplasm and mitochondrial-lysosomal fractions of the heart muscle was studied in the acute experimental period of myocardial ischaemia (5 days) along with a determination of the spectrum of the iso-enzymes of the lactate- and malate- dehydrogenases. For the evaluation of oxidation processes in vitro the iections. Trasylol was found to limit the proteolysis in the cytostructures of the myocardium cell, to increase the activity of the transaminases, as well as to help the normalization of thespectrum of the NAD-dependent iso-enzymes. At the same time, under the effect of the employed drug, the myocardial consumption of the radioactive carbon from the acetate Na-2C-14 increases."}
{"id": "PMID:1079892", "title": "[Intraoperative hemodynamics in patients with pre-infarct stenocardia and acute myocardial infarct].", "content": "Intraoperative haemodynamics was studied in 31 patients with pre-infarction angina pectoris and in 3 patients with acute myocardial infarction. Twelve of them (3 with myocardial infarction) were subjected to a graphic examination of 12 haemodynamic parameters. Aorto-coronary bypass procedures using autovenous grafts were demonstrated to improve the contractile function of the left-ventricular myocardium in this group of patients, as manifested in a decrease of the binite diastolic pressure, increase of the cardiac index and stroke volume in the aorta. Tracing the intraoperative haemodynamics helps in determining the immediate postoperative prognosis and in choosing the optimum regimens of drug therapy. The determination of the volume blood flow in the bypass with the help of a flowmeter is a compulsory procedure, since it permits an objective assessment of the adequacy of the conducted revascularization of the myocardium.", "contents": "[Intraoperative hemodynamics in patients with pre-infarct stenocardia and acute myocardial infarct]. Intraoperative haemodynamics was studied in 31 patients with pre-infarction angina pectoris and in 3 patients with acute myocardial infarction. Twelve of them (3 with myocardial infarction) were subjected to a graphic examination of 12 haemodynamic parameters. Aorto-coronary bypass procedures using autovenous grafts were demonstrated to improve the contractile function of the left-ventricular myocardium in this group of patients, as manifested in a decrease of the binite diastolic pressure, increase of the cardiac index and stroke volume in the aorta. Tracing the intraoperative haemodynamics helps in determining the immediate postoperative prognosis and in choosing the optimum regimens of drug therapy. The determination of the volume blood flow in the bypass with the help of a flowmeter is a compulsory procedure, since it permits an objective assessment of the adequacy of the conducted revascularization of the myocardium."}
{"id": "PMID:1079893", "title": "[Comparative research at the \"mesoptometer\" and \"nyktomat\" (author's transl)].", "content": "This paper deals with the results of comparative reserach into twilight vision as found by tests at the \"Mesoptometer\" and \"Nyktomat\". The research population consisted of 168 persons. Their reactions at the test instruments during various functional tests are demonstrated by means of diagrams. While there is good correspondence between the two test instruments in the range of contrast essential for attesting night driving ability, differences can be shown to exist in the critical range of marginal values.", "contents": "[Comparative research at the \"mesoptometer\" and \"nyktomat\" (author's transl)]. This paper deals with the results of comparative reserach into twilight vision as found by tests at the \"Mesoptometer\" and \"Nyktomat\". The research population consisted of 168 persons. Their reactions at the test instruments during various functional tests are demonstrated by means of diagrams. While there is good correspondence between the two test instruments in the range of contrast essential for attesting night driving ability, differences can be shown to exist in the critical range of marginal values."}
{"id": "PMID:1079894", "title": "[Action of different components of the kallikrein kinin-system on sperm motility in vitro (author's transl)].", "content": "In vitro experiments with human semen showed a significant increase of quantitative and qualitative sperm motility after addition of kallikrein at concentrations of 1 KU/ml. Stimulation of sperm motility was further increased and extended by addition of human serum as a source of kininogen. Kinins added directly also showed enhancement of sperm motility. In contrast, carboxypeptidase B, a kininase, significantly reduced sperm motility. The physiological and therapeutical aspects of these results are discussed.", "contents": "[Action of different components of the kallikrein kinin-system on sperm motility in vitro (author's transl)]. In vitro experiments with human semen showed a significant increase of quantitative and qualitative sperm motility after addition of kallikrein at concentrations of 1 KU/ml. Stimulation of sperm motility was further increased and extended by addition of human serum as a source of kininogen. Kinins added directly also showed enhancement of sperm motility. In contrast, carboxypeptidase B, a kininase, significantly reduced sperm motility. The physiological and therapeutical aspects of these results are discussed."}
{"id": "PMID:1079895", "title": "Hereditary alpha1--antitrypsin deficiency associated with congenital extrahepatic bile duct hypoplasia.", "content": "A child, two months of age, suffering from cholestasis and biliary cirrhosis, was found to be homozygous for alpha1-antitrypsin deficiency Pi type ZZ associated with high degree extrahepatic bile duct obstruction. The clinical, protein-chemical, genetic, histological and immuno-histochemical findings in the patient are reported and the relationships between these two anomalies interpreted. An interaction between the very rare defects rather than random association is suggested.", "contents": "Hereditary alpha1--antitrypsin deficiency associated with congenital extrahepatic bile duct hypoplasia. A child, two months of age, suffering from cholestasis and biliary cirrhosis, was found to be homozygous for alpha1-antitrypsin deficiency Pi type ZZ associated with high degree extrahepatic bile duct obstruction. The clinical, protein-chemical, genetic, histological and immuno-histochemical findings in the patient are reported and the relationships between these two anomalies interpreted. An interaction between the very rare defects rather than random association is suggested."}
{"id": "PMID:1079898", "title": "Narrowing the gap between medical and mental health evaluation.", "content": "Some of the problems facing evaluators of medical care have been dealt with by individuals examining mental health programs. Mental health research has focused more on outcome studies that include a multidimensional approach in evaluation. Techniques have been devised in that field that make the evaluative process patient specific and goal oriented. Borrowing some of these methods to make medical care evaluation include a problem-oriented outcome, which incorporates patient satisfaction, compliance with medical therapy, and relief of symptoms, could lead to more accurate data on the health delivery system.", "contents": "Narrowing the gap between medical and mental health evaluation. Some of the problems facing evaluators of medical care have been dealt with by individuals examining mental health programs. Mental health research has focused more on outcome studies that include a multidimensional approach in evaluation. Techniques have been devised in that field that make the evaluative process patient specific and goal oriented. Borrowing some of these methods to make medical care evaluation include a problem-oriented outcome, which incorporates patient satisfaction, compliance with medical therapy, and relief of symptoms, could lead to more accurate data on the health delivery system."}
{"id": "PMID:1079899", "title": "Approaches to the management of fulminant hepatic failure.", "content": "This discussion has concerned the management of fulminant hepatic failure with stage III or IV encephalopathy occurring in a patient with previously normal liver function. Basic medical management must include special attention to the metabolic, neurologic, hemorrhagic, and infectious complications common to this syndrome as well as aggressive supportive care. Because the mortality rate remains high in spite of these measures, a variety of other approaches to therapy continue to be evaluated. These include corticosteroid administration, exchange transfusion, plasmapheresis, and the development of extracorporeal hepatic support systems.", "contents": "Approaches to the management of fulminant hepatic failure. This discussion has concerned the management of fulminant hepatic failure with stage III or IV encephalopathy occurring in a patient with previously normal liver function. Basic medical management must include special attention to the metabolic, neurologic, hemorrhagic, and infectious complications common to this syndrome as well as aggressive supportive care. Because the mortality rate remains high in spite of these measures, a variety of other approaches to therapy continue to be evaluated. These include corticosteroid administration, exchange transfusion, plasmapheresis, and the development of extracorporeal hepatic support systems."}
{"id": "PMID:1079927", "title": "A diurnal rhythm in melanocyte-stimulating hormone content of the rat pituitary gland and its independence from the pineal gland.", "content": "Resting pituitary melanocyte-stimulating hormone (MSH) content was studied in adult rats kept under a controlled 14 h light-10 h dark regimen. A diurnal rhythm was found in pituitary MSH levels, showing peak values at 4.00-6.00 p.m., and low values during darkness and the beginning of the light period. The normally occurring fall in pituitary MSH content between 6.00 p.m. and midnight was also found in rats exposed to a prolonged photoperiod. Sympathetic denervation of the pineal gland by superior cervical ganglionectomy, as well as pinealectomy, did not block the rhythm in pituitary MSH, and had no effect on pituitary MSH content. In addition, melatonin administered i.v. did not affect pituitary MSH levels, either when MSH activity was tested on the Anolis skin in vitro, or when the in vivo assay with hypophysectomized frogs (Rana pipiens) was used. It was concluded that the diurnal rhythm in pituitary MSH content is not directly related to the light-dark cycle and is not controlled by pineal melatonin or any otherpineal factor.", "contents": "A diurnal rhythm in melanocyte-stimulating hormone content of the rat pituitary gland and its independence from the pineal gland. Resting pituitary melanocyte-stimulating hormone (MSH) content was studied in adult rats kept under a controlled 14 h light-10 h dark regimen. A diurnal rhythm was found in pituitary MSH levels, showing peak values at 4.00-6.00 p.m., and low values during darkness and the beginning of the light period. The normally occurring fall in pituitary MSH content between 6.00 p.m. and midnight was also found in rats exposed to a prolonged photoperiod. Sympathetic denervation of the pineal gland by superior cervical ganglionectomy, as well as pinealectomy, did not block the rhythm in pituitary MSH, and had no effect on pituitary MSH content. In addition, melatonin administered i.v. did not affect pituitary MSH levels, either when MSH activity was tested on the Anolis skin in vitro, or when the in vivo assay with hypophysectomized frogs (Rana pipiens) was used. It was concluded that the diurnal rhythm in pituitary MSH content is not directly related to the light-dark cycle and is not controlled by pineal melatonin or any otherpineal factor."}
{"id": "PMID:1079929", "title": "[Thymolytic activity of chlorimipramine].", "content": "Chloroimipramine in vitro on human lymphocytes stimulated by phytohaemoagglutinin at concentrations from 1 to 10 g/ml leads to mitotic block. Treatment of normal or suprarenalectomized rats with high doses of the drug administered orally leads to a reduction in the weight of the thymus. The hypothesis that chloroimipramine possibly plays a part in immunitary processes is put forward.", "contents": "[Thymolytic activity of chlorimipramine]. Chloroimipramine in vitro on human lymphocytes stimulated by phytohaemoagglutinin at concentrations from 1 to 10 g/ml leads to mitotic block. Treatment of normal or suprarenalectomized rats with high doses of the drug administered orally leads to a reduction in the weight of the thymus. The hypothesis that chloroimipramine possibly plays a part in immunitary processes is put forward."}
{"id": "PMID:1079930", "title": "Maternal and fetal outcome associated with hemoglobin E trait and hemoglobin E disease.", "content": "A study of 20 patients with hemoglobin E trait abnormality and 8 patients with hemoglobin E disease in pregnancy is presented. The hematologic abnormality in HbE hemoglobinopathy is mild though hemoglobin values are significantly lower than in HbA patients. Fetal wastage is no  increased and the salvage rate is high (live births over 95 per cent). The mean birthweight of the baby in HbE patients is significantly lower than in HbA patients, but the incidence of prematurity is not significantly increased in HbE patients. Blood loss at delivery is not markedly affected by hemoglobin type. The incidences of postpartum hemorrhage, preeclampsia, and malaria are not significantly increased in HbE patients; the reason for the significant increase in incidence of abruptio placentae in HbE is not known. No maternal deaths or congenital fetal abnormalities were recorded. It is concluded that the course of pregnancy in HbE trait abnormality and HbE disease is relatively benign.", "contents": "Maternal and fetal outcome associated with hemoglobin E trait and hemoglobin E disease. A study of 20 patients with hemoglobin E trait abnormality and 8 patients with hemoglobin E disease in pregnancy is presented. The hematologic abnormality in HbE hemoglobinopathy is mild though hemoglobin values are significantly lower than in HbA patients. Fetal wastage is no  increased and the salvage rate is high (live births over 95 per cent). The mean birthweight of the baby in HbE patients is significantly lower than in HbA patients, but the incidence of prematurity is not significantly increased in HbE patients. Blood loss at delivery is not markedly affected by hemoglobin type. The incidences of postpartum hemorrhage, preeclampsia, and malaria are not significantly increased in HbE patients; the reason for the significant increase in incidence of abruptio placentae in HbE is not known. No maternal deaths or congenital fetal abnormalities were recorded. It is concluded that the course of pregnancy in HbE trait abnormality and HbE disease is relatively benign."}
{"id": "PMID:1079931", "title": "Mycotic keratitis by Fusarium. A review and report of two cases.", "content": "World literature on the subject reports 112 cases of keratomycosis by Fusarium, including the two cases described in this paper and some personal communications. In most of these cases (60--70%) there is a history of trauma inflicted by vegetable matter or by foreign bodies introduced into the eye (metal, sand, stone, etc.). The different species of Fusarium isolated are F. episphaeria, F. dimerum, F. moniliforme, F. nivale, F. oxysporum, and F. solani. The treatment recommended in a 5-percent suspension or unguent of pimaricin (natamycin).", "contents": "Mycotic keratitis by Fusarium. A review and report of two cases. World literature on the subject reports 112 cases of keratomycosis by Fusarium, including the two cases described in this paper and some personal communications. In most of these cases (60--70%) there is a history of trauma inflicted by vegetable matter or by foreign bodies introduced into the eye (metal, sand, stone, etc.). The different species of Fusarium isolated are F. episphaeria, F. dimerum, F. moniliforme, F. nivale, F. oxysporum, and F. solani. The treatment recommended in a 5-percent suspension or unguent of pimaricin (natamycin)."}
{"id": "PMID:1079940", "title": "Common gastroenterological problems. II.--Sliding hiatus hernia.", "content": "A sliding hiatus hernia is a common radiological finding and is not always relevant to the patient's symptoms. The possibility of an alternative explantation for the complaint of retrosternal pain or dyspepsia should always be considered, and when anaemia is present the site of occult blood loss is often lower in the gastrointestinal tract. The majority of patient's with symptomatic gastro-oesophageal reflux can be controlled with medical measures. Surgical intervention in cases of uncomplicated hiatus hernia should be recommended only after careful preoperative assessment and even then a satisfactory result cannot be absolutely guaranteed.", "contents": "Common gastroenterological problems. II.--Sliding hiatus hernia. A sliding hiatus hernia is a common radiological finding and is not always relevant to the patient's symptoms. The possibility of an alternative explantation for the complaint of retrosternal pain or dyspepsia should always be considered, and when anaemia is present the site of occult blood loss is often lower in the gastrointestinal tract. The majority of patient's with symptomatic gastro-oesophageal reflux can be controlled with medical measures. Surgical intervention in cases of uncomplicated hiatus hernia should be recommended only after careful preoperative assessment and even then a satisfactory result cannot be absolutely guaranteed."}
{"id": "PMID:1079943", "title": "Acute pancreatitis.", "content": "A general survey of acute pancreatitis has been given, with the emphasis on diagnosis and management. Attention is drawn to the difficulties that sometimes arise in the accurate diagnosis of the acute upper abdominal illness and in the assessment of complications arising during the treatment of acute pancreatitis. The importance of the routine use of 'Trasylol' (aprotinin) during the acute attack is emphasized. The indications for operation are discussed in relation to achieving accurate early diagnosis, the management of certain complications and the prevention of further attacks. The mortality rate of acute pancreatitis has dropped considerably with improvement in the diagnostic rate and the routine use of intensive conservative treatment, but the prognosis of septic complications is still grave and will be improved only by the universal recognition that pancreatic suppuration is no ordinary septic process and that the timing of surgical drainage is critical.", "contents": "Acute pancreatitis. A general survey of acute pancreatitis has been given, with the emphasis on diagnosis and management. Attention is drawn to the difficulties that sometimes arise in the accurate diagnosis of the acute upper abdominal illness and in the assessment of complications arising during the treatment of acute pancreatitis. The importance of the routine use of 'Trasylol' (aprotinin) during the acute attack is emphasized. The indications for operation are discussed in relation to achieving accurate early diagnosis, the management of certain complications and the prevention of further attacks. The mortality rate of acute pancreatitis has dropped considerably with improvement in the diagnostic rate and the routine use of intensive conservative treatment, but the prognosis of septic complications is still grave and will be improved only by the universal recognition that pancreatic suppuration is no ordinary septic process and that the timing of surgical drainage is critical."}
{"id": "PMID:1079948", "title": "Effect of hamycin on plasma cholesterol of rat.", "content": "Hamycin, a polyene antibiotic, when administered orally with food or by a cannula or administered intraperitoneally produced a dose-dependent hypocholesterolemic effect on normal albino rats. A 50% decrease occurred in serum cholesterol level at 25-50 mg/kg oral dose of hamycin. No further decrease occurred in serum cholesterol level with increase in the oral dosage. Decrease in the serum cholesterol occurring with 0.5-1.0 mg/kg, ip dose of the antibiotic was similar to that observed with oral hamycin and suggest that the effect was possibly mediated through mechanisms other than those involving prevention of absorption of cholesterol through the gastrointestinal tract.", "contents": "Effect of hamycin on plasma cholesterol of rat. Hamycin, a polyene antibiotic, when administered orally with food or by a cannula or administered intraperitoneally produced a dose-dependent hypocholesterolemic effect on normal albino rats. A 50% decrease occurred in serum cholesterol level at 25-50 mg/kg oral dose of hamycin. No further decrease occurred in serum cholesterol level with increase in the oral dosage. Decrease in the serum cholesterol occurring with 0.5-1.0 mg/kg, ip dose of the antibiotic was similar to that observed with oral hamycin and suggest that the effect was possibly mediated through mechanisms other than those involving prevention of absorption of cholesterol through the gastrointestinal tract."}
{"id": "PMID:1079949", "title": "Time and dose influences on the behavioral effects of L-DOPA and 5-hydroxytryptophan after inhibition of extracerebral decarboxylase.", "content": "The influence of time and dose factors on the locomotor activity and gross behavioral effects of l-3,4-dihydroxyphenylalanine (L-DOPA) and 5-hydroxytryptophan (5-HTP) were assessed a albino mice pretreated with the peripheral decarboxylase inhibitor, MK-486, L-DOPA caused stereotyped behaviors and a decrease in orienting responses and locomotor activity, followed at the highest dose by increased locomotor activity. 5-HTP caused a dose-dependent increase in orienting responses and locomotor activity followed after 90 min by a dose-dependent decrease in locomotor activity. Administration of MK-486 and L-DOPA followed in the same animal by 5-HTP resulted in a diminution of the effects seen after either drug alone. This apparent antagonism may result from competition between the two amino acids at sites of membrane transport in the brain.", "contents": "Time and dose influences on the behavioral effects of L-DOPA and 5-hydroxytryptophan after inhibition of extracerebral decarboxylase. The influence of time and dose factors on the locomotor activity and gross behavioral effects of l-3,4-dihydroxyphenylalanine (L-DOPA) and 5-hydroxytryptophan (5-HTP) were assessed a albino mice pretreated with the peripheral decarboxylase inhibitor, MK-486, L-DOPA caused stereotyped behaviors and a decrease in orienting responses and locomotor activity, followed at the highest dose by increased locomotor activity. 5-HTP caused a dose-dependent increase in orienting responses and locomotor activity followed after 90 min by a dose-dependent decrease in locomotor activity. Administration of MK-486 and L-DOPA followed in the same animal by 5-HTP resulted in a diminution of the effects seen after either drug alone. This apparent antagonism may result from competition between the two amino acids at sites of membrane transport in the brain."}
{"id": "PMID:1079954", "title": "The Syndrome of Enlarged Vestibule and Dysplasia of the Lateral Semicircular Canal in Congenital Deafness.", "content": "A 5-year-old deaf-mute girl was shown on petrous bone tomography to have bilateral enlarged vestibules with assimilation of the lateral semicircular cannals, bilateral absence of the cochlea, aplasia of the left internal auditory canal with only the facial nerve compartment being present, and hypoplasia of the right internal auditory canal. The embryological basis of these inner ear abnormalities is discussed.", "contents": "The Syndrome of Enlarged Vestibule and Dysplasia of the Lateral Semicircular Canal in Congenital Deafness. A 5-year-old deaf-mute girl was shown on petrous bone tomography to have bilateral enlarged vestibules with assimilation of the lateral semicircular cannals, bilateral absence of the cochlea, aplasia of the left internal auditory canal with only the facial nerve compartment being present, and hypoplasia of the right internal auditory canal. The embryological basis of these inner ear abnormalities is discussed."}
{"id": "PMID:1079957", "title": "Intestinal adenomatosis in the pig: a possible relationship with a haemorrhagic enteropathy.", "content": "An outbreak of a haemorrhagic enteropathy in a closed herd of pigs was followed by the appearance, in the same herd, of cases of intestinal adenomatosis. This paper now draws attention to similarties in the mucosal pathology which are present in the two conditions. The affected tissue showed substantial epithelial proliferation and retrospective electron microscopic studies of material from both these entities revealed the presence of bacteria of similar morphology within the cytoplasm of these epithelial cells.", "contents": "Intestinal adenomatosis in the pig: a possible relationship with a haemorrhagic enteropathy. An outbreak of a haemorrhagic enteropathy in a closed herd of pigs was followed by the appearance, in the same herd, of cases of intestinal adenomatosis. This paper now draws attention to similarties in the mucosal pathology which are present in the two conditions. The affected tissue showed substantial epithelial proliferation and retrospective electron microscopic studies of material from both these entities revealed the presence of bacteria of similar morphology within the cytoplasm of these epithelial cells."}
{"id": "PMID:1079961", "title": "[Symptomatic and evolutive characteristics of articular destruction noted in chondrocalcinosis].", "content": "Referring to 17 personal observations, the authors endeavour to clarify the main clinical and radiological traits of the destructive arthropathies occuring in patients suffering from diffuse, articular chondrocalcinosis. These arthropathies appear to be relatively frequent and older, obese women suffering from demineralization are more readily affected. The knees, coxo-femoral joints and the shoulders are principally concerned, and to a lesser extent the wrists, the trapezo-metacarpal joints and even the spine. The lesions can be polyarticular and symmetrical, be grouped in more or less random oligoarticular combinations or may occur in only a single joint space. Clinically, these destructive arthropathies give rise to severe pain and very marked functional impotence. The joints are swollen without any signs of inflammation. The joint movements are painful, stiff, and limited. Axial deviations are frequent. Radiologically, the lesions occur throughout the cartilage sheath, the inter-chondrial bone, and in the underlying epiphysary bone, in the form of massive geodes and massives loss of tissue substance. On the other hand, the process of reconstruction is very limited. In the patients studied, chondrocalcinosis was proved by the very characteristic pictures of calcic incrustations of the cartilage sheath and the fibro-cartilages, by the discovery of micro-crystals of calcium pyrophosphate in the articular fluid or, at biopsy, by the thickness of the synovial fluid. This chondrocalcinosis was primary in the cases. These destructive lesions were easily distinguishable from nervous or diabetic osteo-arthropathies, and from tumoral, infectious, rheumatic, or vascular changes. Thus, chondrocalcinosis is among the most common causes of osteo-articular destruction. It should be looked for systematically in all patients with lytic arthropathies of unknown etiology.", "contents": "[Symptomatic and evolutive characteristics of articular destruction noted in chondrocalcinosis]. Referring to 17 personal observations, the authors endeavour to clarify the main clinical and radiological traits of the destructive arthropathies occuring in patients suffering from diffuse, articular chondrocalcinosis. These arthropathies appear to be relatively frequent and older, obese women suffering from demineralization are more readily affected. The knees, coxo-femoral joints and the shoulders are principally concerned, and to a lesser extent the wrists, the trapezo-metacarpal joints and even the spine. The lesions can be polyarticular and symmetrical, be grouped in more or less random oligoarticular combinations or may occur in only a single joint space. Clinically, these destructive arthropathies give rise to severe pain and very marked functional impotence. The joints are swollen without any signs of inflammation. The joint movements are painful, stiff, and limited. Axial deviations are frequent. Radiologically, the lesions occur throughout the cartilage sheath, the inter-chondrial bone, and in the underlying epiphysary bone, in the form of massive geodes and massives loss of tissue substance. On the other hand, the process of reconstruction is very limited. In the patients studied, chondrocalcinosis was proved by the very characteristic pictures of calcic incrustations of the cartilage sheath and the fibro-cartilages, by the discovery of micro-crystals of calcium pyrophosphate in the articular fluid or, at biopsy, by the thickness of the synovial fluid. This chondrocalcinosis was primary in the cases. These destructive lesions were easily distinguishable from nervous or diabetic osteo-arthropathies, and from tumoral, infectious, rheumatic, or vascular changes. Thus, chondrocalcinosis is among the most common causes of osteo-articular destruction. It should be looked for systematically in all patients with lytic arthropathies of unknown etiology."}
{"id": "PMID:1079962", "title": "[Artificial language, allowing the computer storage of case report abstracts. Applications to the study of a year's examination of 5650 patients in a rheumatology institute].", "content": "An artificial language that makes it possible to put summarized results into a computer. Application of this method to the study of the results of one year consultations with 5650 patients in a institute of rheumatology. The authors present the results of one year's external consulta-with 5650 patients in the Rheumatology Institute of the Faculty of Medicine at Paris Cochin. These results were obtained by computer using an artificial language that made it possible to record and play back a spoken version of the summarized data from the patient's histories.", "contents": "[Artificial language, allowing the computer storage of case report abstracts. Applications to the study of a year's examination of 5650 patients in a rheumatology institute]. An artificial language that makes it possible to put summarized results into a computer. Application of this method to the study of the results of one year consultations with 5650 patients in a institute of rheumatology. The authors present the results of one year's external consulta-with 5650 patients in the Rheumatology Institute of the Faculty of Medicine at Paris Cochin. These results were obtained by computer using an artificial language that made it possible to record and play back a spoken version of the summarized data from the patient's histories."}
{"id": "PMID:1079963", "title": "Factor XIII in a clinical material.", "content": "In 339 patients with various diseases factor XIII (FSF) was determined with the specific amine incorporation method of Lorand et al (1969). Normal values were found in patients with renal (216 patients) or liver diseases (33 patients), in 39 patients with recurrent deep venous thrombosis and in 17 children with congenital cyanotic heart disease. Low levels were found in patients with various conditions, such as sepsis, multiple fractures and combustio complicated by an abnormal proteolytic activity (fibrinolysis and/or activation of the coagulation system with signs of disseminated coagulation). No correlation was found between the FSF and the fibrinogen values or the levels of fibrin/fibrinogen degradation products (FDP). Low FSF values were found in 4 patients with erosive gastritis, with gastrointestinal bleedings and a local fibrinolytic activity in the gastric juice. Although the FSF must be very low (smaller than 1%) if it is to cause bleedings, the low levels in these patients with many other coexisting disturbances in the coagulation system and/or an increased fibrinolytic activity most probably contribute to the increased bleeding tendency in such patients.", "contents": "Factor XIII in a clinical material. In 339 patients with various diseases factor XIII (FSF) was determined with the specific amine incorporation method of Lorand et al (1969). Normal values were found in patients with renal (216 patients) or liver diseases (33 patients), in 39 patients with recurrent deep venous thrombosis and in 17 children with congenital cyanotic heart disease. Low levels were found in patients with various conditions, such as sepsis, multiple fractures and combustio complicated by an abnormal proteolytic activity (fibrinolysis and/or activation of the coagulation system with signs of disseminated coagulation). No correlation was found between the FSF and the fibrinogen values or the levels of fibrin/fibrinogen degradation products (FDP). Low FSF values were found in 4 patients with erosive gastritis, with gastrointestinal bleedings and a local fibrinolytic activity in the gastric juice. Although the FSF must be very low (smaller than 1%) if it is to cause bleedings, the low levels in these patients with many other coexisting disturbances in the coagulation system and/or an increased fibrinolytic activity most probably contribute to the increased bleeding tendency in such patients."}
{"id": "PMID:1079964", "title": "T-Lymphocyte deficiency following adult thymectomin man.", "content": "10 patients thymectomized about 20 years earlier were tested for delayed skin reactivity against mumps, streptokinase-streptodornase and PPD antigens. The T and B lymphocytes in peripheral blood were determined. 6 patients were surgically thymectomized and 2 of the received postoperative irradiation. 4 patients were treated with only high dose irradiation to the mediastinal compartment. None of the patients responded to all skin antigens, 1 was positive to two antigens and 3 to one antigen. The remaining patients were non-responders. The number of peripheral blood lymphocytes was slightly decreased in the patients. A significant decrease in the number of T-lymphocytes (p less than 0.005) was found in the whole patient group, and the depression was more pronounced in the surgically thymectomized patients (p less than 0.001). The number of B-lymphocytes was unaltered. It is concluded that the abnormalities in this patient group reflect a T-lymphocyte deficiency. The immunologic status of the patients before treatment is not known. However, in 3 patients with recently discovered untreated thymomas no abnormalities were found. Thus, adult thymectomy in man seem to lead to deficiency of cell mediated immunity, indicating an influence of the thymus in immunological functions during adult life.", "contents": "T-Lymphocyte deficiency following adult thymectomin man. 10 patients thymectomized about 20 years earlier were tested for delayed skin reactivity against mumps, streptokinase-streptodornase and PPD antigens. The T and B lymphocytes in peripheral blood were determined. 6 patients were surgically thymectomized and 2 of the received postoperative irradiation. 4 patients were treated with only high dose irradiation to the mediastinal compartment. None of the patients responded to all skin antigens, 1 was positive to two antigens and 3 to one antigen. The remaining patients were non-responders. The number of peripheral blood lymphocytes was slightly decreased in the patients. A significant decrease in the number of T-lymphocytes (p less than 0.005) was found in the whole patient group, and the depression was more pronounced in the surgically thymectomized patients (p less than 0.001). The number of B-lymphocytes was unaltered. It is concluded that the abnormalities in this patient group reflect a T-lymphocyte deficiency. The immunologic status of the patients before treatment is not known. However, in 3 patients with recently discovered untreated thymomas no abnormalities were found. Thus, adult thymectomy in man seem to lead to deficiency of cell mediated immunity, indicating an influence of the thymus in immunological functions during adult life."}
{"id": "PMID:1079967", "title": "[Immunological aspects of gastrointestinal neoplasms].", "content": "The mammalian immune system appears to play an essential role in preventing the development, and/or in controlling growth and spread, of malignant tumor cells. Antigenic properties of neoplastic cells and defense mechanism of the tumor host are briefly reviewed. The relative importance of immune reactions and other \"non-specific\" means of host defense with regard to tumor development and growth are discussed in the light of available experimental and clinical evidence. An attempt is made to incorporate these considerations into a hypothesis relating to the development of intestinal neoplasms.", "contents": "[Immunological aspects of gastrointestinal neoplasms]. The mammalian immune system appears to play an essential role in preventing the development, and/or in controlling growth and spread, of malignant tumor cells. Antigenic properties of neoplastic cells and defense mechanism of the tumor host are briefly reviewed. The relative importance of immune reactions and other \"non-specific\" means of host defense with regard to tumor development and growth are discussed in the light of available experimental and clinical evidence. An attempt is made to incorporate these considerations into a hypothesis relating to the development of intestinal neoplasms."}
{"id": "PMID:1079968", "title": "[Malignant tumors of the anal canal and the anal margin].", "content": "81 patients with tumors of the anal canal and anal margin treated at the Cantonal Hospital, Lausanne, during the period January 1942 to June 1974 have been studied. The morbid anatomy of transitional zone and cloacogenic carcinomas, subdivided into basaloid types, is related to the anatomy and histology of the normal anal canal. The average age of the patients was 66.5 years. The incidence of canal tumors was highest in women (71% of cases), but tumors of the anal margin were most frequent in men (75% of cases). The main symptom of anal canal growths in this series of advanced cases was blood on the feces; marginal tumours were associated with anal aching. Cloacogenic carcinomas of the basaloid type have the best prognosis; this is also related to the volume and invasion rate of the tumor, which are features difficult to assess. Melanomas have the worst prognosis; leiomyomas may grow like sarcomas despite their benign histological appearance. 18 patients had inguinal metastases, and only 2 of these are alive 2 1/2 and 3 1/2 years after excision of the rectum; the volume and histological type of the tumor were unrelated to the poor rate of survival. In the light of this experience, rectal amputation is the most effective prodecure for all canal tumors and for most tumors of the margin. Radiotherapy by itself is associated with an unacceptably high risk of actinic proctitis.", "contents": "[Malignant tumors of the anal canal and the anal margin]. 81 patients with tumors of the anal canal and anal margin treated at the Cantonal Hospital, Lausanne, during the period January 1942 to June 1974 have been studied. The morbid anatomy of transitional zone and cloacogenic carcinomas, subdivided into basaloid types, is related to the anatomy and histology of the normal anal canal. The average age of the patients was 66.5 years. The incidence of canal tumors was highest in women (71% of cases), but tumors of the anal margin were most frequent in men (75% of cases). The main symptom of anal canal growths in this series of advanced cases was blood on the feces; marginal tumours were associated with anal aching. Cloacogenic carcinomas of the basaloid type have the best prognosis; this is also related to the volume and invasion rate of the tumor, which are features difficult to assess. Melanomas have the worst prognosis; leiomyomas may grow like sarcomas despite their benign histological appearance. 18 patients had inguinal metastases, and only 2 of these are alive 2 1/2 and 3 1/2 years after excision of the rectum; the volume and histological type of the tumor were unrelated to the poor rate of survival. In the light of this experience, rectal amputation is the most effective prodecure for all canal tumors and for most tumors of the margin. Radiotherapy by itself is associated with an unacceptably high risk of actinic proctitis."}
{"id": "PMID:1079972", "title": "Improved myocardial performance following high 2-3 diphosphoglycerate red cell transfusions.", "content": "Twenty-two matched coronary bypass surgery (CABS) patients were randomly divided into two groups; 11 patients in the control group received CPD-stored or fresh blood (mean age, 5.5 days) containing 70 percent of normal 2-3 diphosphoglycerate (2-3 DPG) and 11 patients received 2-3 DPG-enriched previously frozen, washed, concentrated red cells (2-3 DPG 150 percent of normal). Coming off cardiopulmonary bypass, when given a volume load, the high 2-3 DPG patients had a significant increase in cardiac index (2.95 L. per minute vs. 2.18 L. per minute, p smaller than 0.001) at similar filling pressures. At this time body oxygen consumption, in vivo P50, red cell 2-3 DPG, and arterial-venous oxygen content difference were all increased, but P minus vo2 was normal. Improved oxygen delivery occurred without decreasing mixed venous oxygen tension. The results suggest that, with volume loading, function in the heart with coronary artery disease is limited in part by available oxygen. By decreasing oxygen affinity for hemoglobin by altering red blood cells biochemically, myocardial performance can be improved safely.", "contents": "Improved myocardial performance following high 2-3 diphosphoglycerate red cell transfusions. Twenty-two matched coronary bypass surgery (CABS) patients were randomly divided into two groups; 11 patients in the control group received CPD-stored or fresh blood (mean age, 5.5 days) containing 70 percent of normal 2-3 diphosphoglycerate (2-3 DPG) and 11 patients received 2-3 DPG-enriched previously frozen, washed, concentrated red cells (2-3 DPG 150 percent of normal). Coming off cardiopulmonary bypass, when given a volume load, the high 2-3 DPG patients had a significant increase in cardiac index (2.95 L. per minute vs. 2.18 L. per minute, p smaller than 0.001) at similar filling pressures. At this time body oxygen consumption, in vivo P50, red cell 2-3 DPG, and arterial-venous oxygen content difference were all increased, but P minus vo2 was normal. Improved oxygen delivery occurred without decreasing mixed venous oxygen tension. The results suggest that, with volume loading, function in the heart with coronary artery disease is limited in part by available oxygen. By decreasing oxygen affinity for hemoglobin by altering red blood cells biochemically, myocardial performance can be improved safely."}
{"id": "PMID:1079975", "title": "[Patho-histological findings on pulmonary biopsy in patients after mitral commissurotomy and morphometric studies of the upper and lower pulmonary lobes (author's transl)].", "content": "Histological morphometric examination of the lungs of 22 patients with acquired mitral stenosis and 15 control persons is reported. The quotient obtained from wall thickness and lumen diameter of the pulmonary venules gives a reliable estimation of the haemodynamic changes found in the pulmonary vascular system in mitral stenosis patients. The typical changes in the pulmonary vascular system in these patients were observed particularly in the lower pulmonary lobe and to a lesser extent in the lingular of the upper lobe. Biopsy of the lower lobe is preferable in mitral stenosis operations because it results in a better clinical diagnosis.", "contents": "[Patho-histological findings on pulmonary biopsy in patients after mitral commissurotomy and morphometric studies of the upper and lower pulmonary lobes (author's transl)]. Histological morphometric examination of the lungs of 22 patients with acquired mitral stenosis and 15 control persons is reported. The quotient obtained from wall thickness and lumen diameter of the pulmonary venules gives a reliable estimation of the haemodynamic changes found in the pulmonary vascular system in mitral stenosis patients. The typical changes in the pulmonary vascular system in these patients were observed particularly in the lower pulmonary lobe and to a lesser extent in the lingular of the upper lobe. Biopsy of the lower lobe is preferable in mitral stenosis operations because it results in a better clinical diagnosis."}
{"id": "PMID:1079976", "title": "[Electrode problems in pacemaker wearers (experiences with three electrode types) (author's transl)].", "content": "3 types of electrodes (Medtronic 6903, EMT 588, MIP 2000) of 123 patients are examined by following criterial 1. rate of dislocation, 2. development of threshold, 3. time of application. We found: The Medtronic has the smallest rate of dislocation, the lowest range of threshold and is quickly placed in the right ventricle. The rate of dislocation of the EMT 588 is still just acceptable and it also has a sufficient low threshold, but in comparison to the other helical-wire electrodes the disadvantage of being rather difficult to place in the right ventricle. The MIP 2000 has not proved to be useful due to its large rate of dislocation.", "contents": "[Electrode problems in pacemaker wearers (experiences with three electrode types) (author's transl)]. 3 types of electrodes (Medtronic 6903, EMT 588, MIP 2000) of 123 patients are examined by following criterial 1. rate of dislocation, 2. development of threshold, 3. time of application. We found: The Medtronic has the smallest rate of dislocation, the lowest range of threshold and is quickly placed in the right ventricle. The rate of dislocation of the EMT 588 is still just acceptable and it also has a sufficient low threshold, but in comparison to the other helical-wire electrodes the disadvantage of being rather difficult to place in the right ventricle. The MIP 2000 has not proved to be useful due to its large rate of dislocation."}
{"id": "PMID:1079977", "title": "[Valvular replacement in florid endocarditis. Report on 5 cases].", "content": "This is an account of experience with valve replacement in patients with active endocarditis. In four patients the aortic valve was replaced, and in one, the mitral valve. Indication for surgery in all five cases was heart insufficiency. Two patients died, one of acute left heart failure five weeks postoperatively, and one of brain embolus in the presence of persisting acute endocarditis, four months postoperatively. Covered in the discussion are other indications for valve replacement in the presence of acute endocarditis, namely, serial emboli, fungus endocarditis, resistance to and toxic reactions of antibiotics, and mycotic sinus of valsava aneurysm. In conclusion, results obtained by other authors are reported.", "contents": "[Valvular replacement in florid endocarditis. Report on 5 cases]. This is an account of experience with valve replacement in patients with active endocarditis. In four patients the aortic valve was replaced, and in one, the mitral valve. Indication for surgery in all five cases was heart insufficiency. Two patients died, one of acute left heart failure five weeks postoperatively, and one of brain embolus in the presence of persisting acute endocarditis, four months postoperatively. Covered in the discussion are other indications for valve replacement in the presence of acute endocarditis, namely, serial emboli, fungus endocarditis, resistance to and toxic reactions of antibiotics, and mycotic sinus of valsava aneurysm. In conclusion, results obtained by other authors are reported."}
{"id": "PMID:1079978", "title": "[Thrombosis of the Bj\u00f6rk-Shiley valve as an early fatal complication of the prothetic aortic valve replacement (author's transl)].", "content": "From a series of 28 patients subjected to aortic valve replacement with the Bj\u00f6rk-Shiley tilting disc valve prosthesis 3 cases are reported in which massive thrombosis with valve dysfunction and fatal outcome occurred in the very early postoperative period. The autopsy findings and the examination of the blood coagulation state during the first 24 hours postoperatively disclosed in all 3 cases a hypercoagulability being responsible for the massive clot formation. The early recognition and establishment of the diagnosis of a hypercoagulability state and an immediate anticoagulant therapy with heparin are necessary for preventing such early fatal complications in patients with cardiac valve replacement.", "contents": "[Thrombosis of the Bj\u00f6rk-Shiley valve as an early fatal complication of the prothetic aortic valve replacement (author's transl)]. From a series of 28 patients subjected to aortic valve replacement with the Bj\u00f6rk-Shiley tilting disc valve prosthesis 3 cases are reported in which massive thrombosis with valve dysfunction and fatal outcome occurred in the very early postoperative period. The autopsy findings and the examination of the blood coagulation state during the first 24 hours postoperatively disclosed in all 3 cases a hypercoagulability being responsible for the massive clot formation. The early recognition and establishment of the diagnosis of a hypercoagulability state and an immediate anticoagulant therapy with heparin are necessary for preventing such early fatal complications in patients with cardiac valve replacement."}
{"id": "PMID:1079979", "title": "[Preliminary experiences in use of the Sparks prosthesis for femoro-popliteal bypass and arterio-venous shunt in chronic hemodialysis (author's transl)].", "content": "The present paper summarizes our preliminary results using the Sparks-Mandril. The prothesis has been applied both as femoro-popliteal bypass graft and as arteriovenous shunt for hemodialysis in patients suffering from chronic renal failure. Indications, techniques and results of implantation both as femoro-popliteal bypass and as a.-v. shunt are outlined. It is concluded that in both cases the Sparks-Mandril has its well defined field of application.", "contents": "[Preliminary experiences in use of the Sparks prosthesis for femoro-popliteal bypass and arterio-venous shunt in chronic hemodialysis (author's transl)]. The present paper summarizes our preliminary results using the Sparks-Mandril. The prothesis has been applied both as femoro-popliteal bypass graft and as arteriovenous shunt for hemodialysis in patients suffering from chronic renal failure. Indications, techniques and results of implantation both as femoro-popliteal bypass and as a.-v. shunt are outlined. It is concluded that in both cases the Sparks-Mandril has its well defined field of application."}
{"id": "PMID:1079980", "title": "[Mechanisms of transmitting excitation in the electromechanical connections system of frog phasic muscle fibers].", "content": "The effect of replacement of Na ions by isomorphic Li ions on the dynamic parameters of isometric contractions and the distribution of Na, K, and Li in the frog m. sartorius involved increase of the muscle maximum tension during first 10-15 min. A possible role of Na and Li ions in the muscle contraction is discussed with regard to the assumption of a mechanism of participation of Na ions in the transmission of excitation from the plasmic membrane to the sarcoplasmic reticulum membrane.", "contents": "[Mechanisms of transmitting excitation in the electromechanical connections system of frog phasic muscle fibers]. The effect of replacement of Na ions by isomorphic Li ions on the dynamic parameters of isometric contractions and the distribution of Na, K, and Li in the frog m. sartorius involved increase of the muscle maximum tension during first 10-15 min. A possible role of Na and Li ions in the muscle contraction is discussed with regard to the assumption of a mechanism of participation of Na ions in the transmission of excitation from the plasmic membrane to the sarcoplasmic reticulum membrane."}
{"id": "PMID:1079986", "title": "Removal of leukocytes from whole blood and erythrocyte suspensions by filtration through cotton wool. V. Results after transfusion of 1,820 units of filtered erythrocytes.", "content": "1,820 units of leukocyte- and platelet-poor erythrocyte suspensions were prepared by filtration through cotton wool. On the average more than 98% of the leukocytes and 90-95% of the platelets could be removed. The red cell recovery was 96%. 97% of the units given to polytransfused patients did not cause febrile reactions. Serological follow-up of future transplantation recipients indicated that the immunization may be avoided by using erythrocyte suspensions of fresh, filtered blood.", "contents": "Removal of leukocytes from whole blood and erythrocyte suspensions by filtration through cotton wool. V. Results after transfusion of 1,820 units of filtered erythrocytes. 1,820 units of leukocyte- and platelet-poor erythrocyte suspensions were prepared by filtration through cotton wool. On the average more than 98% of the leukocytes and 90-95% of the platelets could be removed. The red cell recovery was 96%. 97% of the units given to polytransfused patients did not cause febrile reactions. Serological follow-up of future transplantation recipients indicated that the immunization may be avoided by using erythrocyte suspensions of fresh, filtered blood."}
{"id": "PMID:1079987", "title": "Platelet indirect radioactive Coombs test. Its utilization for PLA1 grouping.", "content": "A platelet indirect radioactive Coombs test (PIRC) has been described. The technique for purification and labelling the antiglobulin has been precised. This test allowed the typing of platelets in the PLA system by using an absorbed serum from a mother of a thrombocytopenic child. Six other families of neonatal thrombocytopenias were tested. In three of them, the mothers were found PLA1 negative (PLA2, PLA2). Among a panel of 93 platelets, two (0.022) were found PLA1, negative. This PIRC test has many advantages compared to other tests such as platelet complement fixation, assay for blocking antibodies or antiglobulin consumption: it gives objective and quantitative results and is highly reproducible; anticomplementary serum may be tested.", "contents": "Platelet indirect radioactive Coombs test. Its utilization for PLA1 grouping. A platelet indirect radioactive Coombs test (PIRC) has been described. The technique for purification and labelling the antiglobulin has been precised. This test allowed the typing of platelets in the PLA system by using an absorbed serum from a mother of a thrombocytopenic child. Six other families of neonatal thrombocytopenias were tested. In three of them, the mothers were found PLA1 negative (PLA2, PLA2). Among a panel of 93 platelets, two (0.022) were found PLA1, negative. This PIRC test has many advantages compared to other tests such as platelet complement fixation, assay for blocking antibodies or antiglobulin consumption: it gives objective and quantitative results and is highly reproducible; anticomplementary serum may be tested."}
{"id": "PMID:1079992", "title": "Interstital nephritis in autoimmune hemolytic anemia.", "content": "A case is reported of a 15-year-old boy with chronic autoimmune hemolytic anemia who developed renal insufficiency 3 years after splenectomy. An interstitial nephritis with striking lymphocytic infiltrates and sclerosed glomeruli could be demonstrated by percutaneous renal biopsy. Renal symptoms disappeared promptly after corticosteroid therapy. The renal lesions are thought to have arisen as part of the autoimmune disease.", "contents": "Interstital nephritis in autoimmune hemolytic anemia. A case is reported of a 15-year-old boy with chronic autoimmune hemolytic anemia who developed renal insufficiency 3 years after splenectomy. An interstitial nephritis with striking lymphocytic infiltrates and sclerosed glomeruli could be demonstrated by percutaneous renal biopsy. Renal symptoms disappeared promptly after corticosteroid therapy. The renal lesions are thought to have arisen as part of the autoimmune disease."}
{"id": "PMID:1079994", "title": "Pneumocystis carinii infections in zoo animals.", "content": "Pneumocystis carinii was found to be present in the lungs of twenty-three zoo animals in the Netherlands. The following species were represented: red kangaroo, common tree shrew, Senegal-Galago, Demidoff's-Galago, brown howler monkey, woolly monkey, long-haired spider monkey, white-eared marmoset, chimpanzee, three-toed sloth, palm squirrel, red panda, fennec fox, tree hyrax and large-toothed hyrax.", "contents": "Pneumocystis carinii infections in zoo animals. Pneumocystis carinii was found to be present in the lungs of twenty-three zoo animals in the Netherlands. The following species were represented: red kangaroo, common tree shrew, Senegal-Galago, Demidoff's-Galago, brown howler monkey, woolly monkey, long-haired spider monkey, white-eared marmoset, chimpanzee, three-toed sloth, palm squirrel, red panda, fennec fox, tree hyrax and large-toothed hyrax."}
{"id": "PMID:1079995", "title": "Effect of trasylol on packed cell volume and plasma kallikrein activation in acute Babesia argentina infection of splenectomised calves.", "content": "Three groups of four splenectomised calves, three months old, were infected with Babesia argentina parasites. One group was inoculated intravenously with 2000 kallikrein inhibitor units (kiu)/kg body weight of the broad-spectrum proteinase inhibitor, Trasylol, three times a day for 5 days commencing on the 3rd day after infection (early treatment). The same dose of Trasylol was given to a second group for 3 days commencing 8 days after infection (late treatment). The third group was untreated. Parasite multiplication rates were similar in the three groups. In the early treated group levels of activated kallikrein in plasma were significantly lower than those in the other two groups. The early treatment group also showed significantly higher levels of plasma kallikrein inhibitor. No significant differences in total plasma kallikrein levels were seen among the three groups. Packed cell volumes in the treated groups remained significantly higher than those in the controls. The implications of these findings are discussed.", "contents": "Effect of trasylol on packed cell volume and plasma kallikrein activation in acute Babesia argentina infection of splenectomised calves. Three groups of four splenectomised calves, three months old, were infected with Babesia argentina parasites. One group was inoculated intravenously with 2000 kallikrein inhibitor units (kiu)/kg body weight of the broad-spectrum proteinase inhibitor, Trasylol, three times a day for 5 days commencing on the 3rd day after infection (early treatment). The same dose of Trasylol was given to a second group for 3 days commencing 8 days after infection (late treatment). The third group was untreated. Parasite multiplication rates were similar in the three groups. In the early treated group levels of activated kallikrein in plasma were significantly lower than those in the other two groups. The early treatment group also showed significantly higher levels of plasma kallikrein inhibitor. No significant differences in total plasma kallikrein levels were seen among the three groups. Packed cell volumes in the treated groups remained significantly higher than those in the controls. The implications of these findings are discussed."}
{"id": "PMID:1079996", "title": "[Effect of primaricin on moulds and their aflatoxin formation in cheese].", "content": "The prior condition for the application of pimaricin is its heat stability (up to 80 degrees C) and the low penetration (around 2.6 mm), so that it is available for the effect on the cheese surface for a long period. With 13 different aflatoxin forming moulds, the inhibition on the mycel development and thereby on the aflatoxin formation was tested; the different strains were inhibited to various degrees. The effect on cheese was not definite, because the native cheese surface remained free of mould for 8 weeks after pimaricin treatment, whereas cheese slices dipped into pimaricin solution were covered with moulds very soon. The aflatoxin formation itself is only inhibited, if the growth of the moulds is inhibited. Therefore the cheeses have to be treated very early before strong growth of the moulds has started. The aflatoxin formation, however only starts at a certain growth period of the moulds, but at a not completed inhibition, a reduced aflatoxin formation has to be taken into consideration.", "contents": "[Effect of primaricin on moulds and their aflatoxin formation in cheese]. The prior condition for the application of pimaricin is its heat stability (up to 80 degrees C) and the low penetration (around 2.6 mm), so that it is available for the effect on the cheese surface for a long period. With 13 different aflatoxin forming moulds, the inhibition on the mycel development and thereby on the aflatoxin formation was tested; the different strains were inhibited to various degrees. The effect on cheese was not definite, because the native cheese surface remained free of mould for 8 weeks after pimaricin treatment, whereas cheese slices dipped into pimaricin solution were covered with moulds very soon. The aflatoxin formation itself is only inhibited, if the growth of the moulds is inhibited. Therefore the cheeses have to be treated very early before strong growth of the moulds has started. The aflatoxin formation, however only starts at a certain growth period of the moulds, but at a not completed inhibition, a reduced aflatoxin formation has to be taken into consideration."}
{"id": "PMID:1079997", "title": "[Conduction disorders in aortic valve diseases].", "content": "Among 304 cases of aortic valvulopathies studied for surgical selection, the authors have found a high incidence of conduction disturbances (16% in aortic stenosis and 18,4% in aortic regurgitation). The conduction defects are mostly intraventricular among stenosis isolated or associated to regurgitation and mostly atrio-ventricular among pure aortic insufficiencies. The highest incidence (30%) being found in patients with bacterial endocarditis acute or healed. The incidence of conduction disturbances increases with age, with the presence of valvular calcifications, of left ventricular strain or failure, of coronary insufficiency and angina... practically with the duration and the severity of the valvular disease. Surgical risk is heavier and natural prognosis poorer in valvulopathies with conduction disturbances. But these disturbances never contraindicates surgery : it is sometimes necessary to insert a pacemaker with or without valvular replacement mostly in aortic stenosis with infrahisian conduction defects. During hemodynamic investigation of such cases one must be ready to stimulate the heart, particularly during right heart catheterization of patients with complete left bundle branch block.", "contents": "[Conduction disorders in aortic valve diseases]. Among 304 cases of aortic valvulopathies studied for surgical selection, the authors have found a high incidence of conduction disturbances (16% in aortic stenosis and 18,4% in aortic regurgitation). The conduction defects are mostly intraventricular among stenosis isolated or associated to regurgitation and mostly atrio-ventricular among pure aortic insufficiencies. The highest incidence (30%) being found in patients with bacterial endocarditis acute or healed. The incidence of conduction disturbances increases with age, with the presence of valvular calcifications, of left ventricular strain or failure, of coronary insufficiency and angina... practically with the duration and the severity of the valvular disease. Surgical risk is heavier and natural prognosis poorer in valvulopathies with conduction disturbances. But these disturbances never contraindicates surgery : it is sometimes necessary to insert a pacemaker with or without valvular replacement mostly in aortic stenosis with infrahisian conduction defects. During hemodynamic investigation of such cases one must be ready to stimulate the heart, particularly during right heart catheterization of patients with complete left bundle branch block."}
{"id": "PMID:1079998", "title": "Concealed retrograde conduction of an atrial echo in second degree A-V block of the Wenckebach type with modified ventricular acceleration.", "content": "Whereas second degree A-V block showing the classical pattern of the Wenckebach phenomenon is easily diagnosed, variant forms of the Wenckebach phenomenon in second degree A-V block may pose difficulties to the interpretor. A case is presented, where modified acceleration of the ventricles--a crucial \"pointer\" to the Wenckebach A-V block -- is disturbed by concealed conduction of an atrial echo. Differences in the electrocardiographic manifestation between this and the classical type of second degree A-V block of the Wenckebach type are discussed.", "contents": "Concealed retrograde conduction of an atrial echo in second degree A-V block of the Wenckebach type with modified ventricular acceleration. Whereas second degree A-V block showing the classical pattern of the Wenckebach phenomenon is easily diagnosed, variant forms of the Wenckebach phenomenon in second degree A-V block may pose difficulties to the interpretor. A case is presented, where modified acceleration of the ventricles--a crucial \"pointer\" to the Wenckebach A-V block -- is disturbed by concealed conduction of an atrial echo. Differences in the electrocardiographic manifestation between this and the classical type of second degree A-V block of the Wenckebach type are discussed."}
{"id": "PMID:1079999", "title": "[Demonstration of a major preexcitation syndrome during treatment of auricular flutter using intravenous injection of verapamil].", "content": "Electrophysiologic properties of various conduction pathways responsible for preexcitation syndrome are illdefined; they explain the incidence of arrhythmias : supraventricular tachycardia, atrial fibrillation and, in much rarer cases, flutter. The site of Verapamil activity is chiefly the atrioventricular junction, while its action on the refractory period of atrio-ventricular bypass is small and unforeseable. In a patient with type B. W.P.W. syndrome, complicated with atrial flutter Verapamil induced a preferential stimulation through the anomalous pathway. Right BBB which was obvious during the attack of atrial flutter, was hidden by the type B W.P.W. syndrome, the right branch being shunted by the anomalous pathway. The infrequency of atrial flutter in type B W.P.W. syndrome is emphasized.", "contents": "[Demonstration of a major preexcitation syndrome during treatment of auricular flutter using intravenous injection of verapamil]. Electrophysiologic properties of various conduction pathways responsible for preexcitation syndrome are illdefined; they explain the incidence of arrhythmias : supraventricular tachycardia, atrial fibrillation and, in much rarer cases, flutter. The site of Verapamil activity is chiefly the atrioventricular junction, while its action on the refractory period of atrio-ventricular bypass is small and unforeseable. In a patient with type B. W.P.W. syndrome, complicated with atrial flutter Verapamil induced a preferential stimulation through the anomalous pathway. Right BBB which was obvious during the attack of atrial flutter, was hidden by the type B W.P.W. syndrome, the right branch being shunted by the anomalous pathway. The infrequency of atrial flutter in type B W.P.W. syndrome is emphasized."}
{"id": "PMID:1080000", "title": "[Arterial vascularization of the sino-auricular node of the heart in dogs].", "content": "In order to study the arterial blood supply of the sinus atrial node in the Dog, the authors have performed the post-mortem injection of the arterial branches going to this node. They observed 65 cases and they came to the following conclusions: in 31 cases (47.7%) the sinus atrial node is supplied by the artery of the sinus atrial node and by the anterior atrial artery, branch of the circumflex; in 20 cases (30.8%) besides the two arteries already mentioned there was also the anterior branch from the first portion of the right coronary artery; in 13 cases (20%) the sinus atrial node was exclusively supplied by the artery of the sinus atrial node and in one case (1.5%) it was supplied only by the first anterior atrial branch of the circumflex artery. Their findings are different from those of other authors and they attribute the failure in provoking experimental ischemia of the sinus atrial node in the Dog, to the rich blood supply of this node and to the different origins of the vessels supplying it.", "contents": "[Arterial vascularization of the sino-auricular node of the heart in dogs]. In order to study the arterial blood supply of the sinus atrial node in the Dog, the authors have performed the post-mortem injection of the arterial branches going to this node. They observed 65 cases and they came to the following conclusions: in 31 cases (47.7%) the sinus atrial node is supplied by the artery of the sinus atrial node and by the anterior atrial artery, branch of the circumflex; in 20 cases (30.8%) besides the two arteries already mentioned there was also the anterior branch from the first portion of the right coronary artery; in 13 cases (20%) the sinus atrial node was exclusively supplied by the artery of the sinus atrial node and in one case (1.5%) it was supplied only by the first anterior atrial branch of the circumflex artery. Their findings are different from those of other authors and they attribute the failure in provoking experimental ischemia of the sinus atrial node in the Dog, to the rich blood supply of this node and to the different origins of the vessels supplying it."}
{"id": "PMID:1080001", "title": "From physical capacity to fitness to work in coronary patients.", "content": "The authors studied the energetic expenditures of 15 coronary patients during work. The subjects with reduced physical capacity tolerated a high fraction of their VO2 SL during their work with activity peaks close to their maximal capacity.", "contents": "From physical capacity to fitness to work in coronary patients. The authors studied the energetic expenditures of 15 coronary patients during work. The subjects with reduced physical capacity tolerated a high fraction of their VO2 SL during their work with activity peaks close to their maximal capacity."}
{"id": "PMID:1080002", "title": "Cardiac manifestations of digoxin intoxication. (Clinical correlations with serum levels).", "content": "Clinical, electrocardiographic and laboratory data were found out in 52 patients with cardiac arrhythmias and conduction defects due to digoxin intoxication. Forty six nontoxic patients were also studied for comparison. Blood urea concentration was significantly higher in toxic patients as compared to nontoxic ones (P less than 0-01). Ventricular bigeminy and trigeminy (38.6%), multifocal ventricular premature beats (25%) and second or third degree A-V blocks (25%) were very much prevalent. Fairly good correlations have been observed between different cardiac arrhythmias and serum digoxin levels. Significantly higher mean serum digoxin levels were observed in patients with A-V block and multifocal ventricular premature beats as compared to patients with supraventricular arrhythmias.", "contents": "Cardiac manifestations of digoxin intoxication. (Clinical correlations with serum levels). Clinical, electrocardiographic and laboratory data were found out in 52 patients with cardiac arrhythmias and conduction defects due to digoxin intoxication. Forty six nontoxic patients were also studied for comparison. Blood urea concentration was significantly higher in toxic patients as compared to nontoxic ones (P less than 0-01). Ventricular bigeminy and trigeminy (38.6%), multifocal ventricular premature beats (25%) and second or third degree A-V blocks (25%) were very much prevalent. Fairly good correlations have been observed between different cardiac arrhythmias and serum digoxin levels. Significantly higher mean serum digoxin levels were observed in patients with A-V block and multifocal ventricular premature beats as compared to patients with supraventricular arrhythmias."}
{"id": "PMID:1080003", "title": "[Prognosis and treatment of bacterial endocarditis. Review of 70 cases].", "content": "The long term results of medical and surgical treatments have been reviewed in 70 cases examined during the last 8 years. In 24 of them, bacterial endocarditis developed after valvular replacement. Antibiotic treatment was administered during at least 6 weeks. Determinations of the minimum inhibitory concentration of the antibiotic and of the bactericidal power of the serum were considered as the most important laboratory controls. In the post-operative cases with prosthetic valves, numerous pathogenic organisms were found, including gram (minus) bacilli and monilia, which may partly account for the high mortality in this group. Despite surgical reinterventions, 20 of the 24 patients died. For the gram (+) cocci, prognosis is however statistically worse (at the 99% confidence limit) when prosthetic valves are present. Out of the 46 patients without prosthetic valves, 31 (68%) had a favourable bacteriological and haemodynamic evolution; in this group, gram (minus) bacilli and monilia were never found to be the pathogenic organisms. For streptococci infections, recovery was obtained in 73% of the cases (24 out of 33). Percentages of long term recovery are very similar in mitral (67%) and aortic (69%) valvular involvment, but the frequency of surgical indication was very different in either group. In cases of aortic valvular disease, valvular replacement was indicated for bacteriological or haemodynamic reasons in 19 of the 20 patients who recovered. Amongst the 9 patients who died, 6 were not operated upon. Valvular replacement is by far less frequently necessary in cases of bacterial endocarditis on the mitral valve : one prosthetic valve amongst 8 patients who recovered. Finally, the authors discuss the place of surgery in the treatment of bacterial endocarditis. According to their experience, recent improvement in the prognosis of the disease seems to depend more on the surgical approach of the problem than on the discovery of new antibiotics.", "contents": "[Prognosis and treatment of bacterial endocarditis. Review of 70 cases]. The long term results of medical and surgical treatments have been reviewed in 70 cases examined during the last 8 years. In 24 of them, bacterial endocarditis developed after valvular replacement. Antibiotic treatment was administered during at least 6 weeks. Determinations of the minimum inhibitory concentration of the antibiotic and of the bactericidal power of the serum were considered as the most important laboratory controls. In the post-operative cases with prosthetic valves, numerous pathogenic organisms were found, including gram (minus) bacilli and monilia, which may partly account for the high mortality in this group. Despite surgical reinterventions, 20 of the 24 patients died. For the gram (+) cocci, prognosis is however statistically worse (at the 99% confidence limit) when prosthetic valves are present. Out of the 46 patients without prosthetic valves, 31 (68%) had a favourable bacteriological and haemodynamic evolution; in this group, gram (minus) bacilli and monilia were never found to be the pathogenic organisms. For streptococci infections, recovery was obtained in 73% of the cases (24 out of 33). Percentages of long term recovery are very similar in mitral (67%) and aortic (69%) valvular involvment, but the frequency of surgical indication was very different in either group. In cases of aortic valvular disease, valvular replacement was indicated for bacteriological or haemodynamic reasons in 19 of the 20 patients who recovered. Amongst the 9 patients who died, 6 were not operated upon. Valvular replacement is by far less frequently necessary in cases of bacterial endocarditis on the mitral valve : one prosthetic valve amongst 8 patients who recovered. Finally, the authors discuss the place of surgery in the treatment of bacterial endocarditis. According to their experience, recent improvement in the prognosis of the disease seems to depend more on the surgical approach of the problem than on the discovery of new antibiotics."}
{"id": "PMID:1080004", "title": "Electronic processes in generation of nerve excitation.", "content": "In a frog sciatic nerve stained by very dilute solution of eosin (0.1 mumol/1 minus to 100 mumol/1) and illuminated with a rather intensive light (10,000--100,000 lux), a single electric stimulus elicited not a single response, but a train of spikes. Latency time and reversibility of the effect, threshold concentration, threshold illumination, frequency and full length of train of spikes were investigated in detail. The results of the experiments are in agreement with the theory according to which the generation of action potentials involves electronic processes.", "contents": "Electronic processes in generation of nerve excitation. In a frog sciatic nerve stained by very dilute solution of eosin (0.1 mumol/1 minus to 100 mumol/1) and illuminated with a rather intensive light (10,000--100,000 lux), a single electric stimulus elicited not a single response, but a train of spikes. Latency time and reversibility of the effect, threshold concentration, threshold illumination, frequency and full length of train of spikes were investigated in detail. The results of the experiments are in agreement with the theory according to which the generation of action potentials involves electronic processes."}
{"id": "PMID:1080006", "title": "Selective coronary arteriography.", "content": "A series of 414 selective coronary arteriographies in 322 patients has been done at Ullev\u00e5l Hospital from May 1971 to Jan. 1974. Judkins technique was used in all cases. The indications for arteriography, the diagnoses as regards the coronary arteries, the therapeutic consequences,and the complications of selective coronary arteriography are described.", "contents": "Selective coronary arteriography. A series of 414 selective coronary arteriographies in 322 patients has been done at Ullev\u00e5l Hospital from May 1971 to Jan. 1974. Judkins technique was used in all cases. The indications for arteriography, the diagnoses as regards the coronary arteries, the therapeutic consequences,and the complications of selective coronary arteriography are described."}
{"id": "PMID:1080007", "title": "Aortocoronary vein bypass in patients with angina pectoris.", "content": "In the 3-year period from May 1971 to April 1974, 90 patients had aortocoronary bypass for angina pectoris at Ulleval Hospital. One patient died shortly after the operation (operative mortality 1.1%). There were no further deaths in the observation period. Clinical improvement was seen in 93% of the patients, early shunt patency in 92%. The study suggests that patients with isolated affection of the right coronary artery should not have bypass, because these patients 1) had less severe symptoms, 2) had better preserved left ventricular function, and 3) seemed to have a smaller chance of benefiting from the operation than the other patients. Multiple shunts gave good clinical results and carried no higher surgical risk than did single shunts. Good clinical results were seen also in patients with occluded shunts provided they had at least one patent shunt too. Graft occlusion occurred early and was associated with low graft flow as measured intraoperatively. Graft occlusion was not usually followed by demonstrable myocardial necrosis. In view of the small operative risk and the high score of symptom relief it is concluded that all patients with angina pectoris that does not readily respond to medical treatment, should be considered for aortocoronary bypass.", "contents": "Aortocoronary vein bypass in patients with angina pectoris. In the 3-year period from May 1971 to April 1974, 90 patients had aortocoronary bypass for angina pectoris at Ulleval Hospital. One patient died shortly after the operation (operative mortality 1.1%). There were no further deaths in the observation period. Clinical improvement was seen in 93% of the patients, early shunt patency in 92%. The study suggests that patients with isolated affection of the right coronary artery should not have bypass, because these patients 1) had less severe symptoms, 2) had better preserved left ventricular function, and 3) seemed to have a smaller chance of benefiting from the operation than the other patients. Multiple shunts gave good clinical results and carried no higher surgical risk than did single shunts. Good clinical results were seen also in patients with occluded shunts provided they had at least one patent shunt too. Graft occlusion occurred early and was associated with low graft flow as measured intraoperatively. Graft occlusion was not usually followed by demonstrable myocardial necrosis. In view of the small operative risk and the high score of symptom relief it is concluded that all patients with angina pectoris that does not readily respond to medical treatment, should be considered for aortocoronary bypass."}
{"id": "PMID:1080008", "title": "Inhibition spectra of the human pancreatic endopeptidases.", "content": "The present work describes the effect of seven naturally occurring proteinase inhibitors on the human pancreatic endopeptidases cationic trypsin, anionic trypsin, chymotrypsin I, chymotrypsin II, and protease E (an elastase-like protease). The inhibitors tested in order of their decreasing effectiveness were alpha-1-proteinase inhibitor (alpha-1-antitrypsin), lima bean trypsin inhibitor, soybean trypsin inhibitor, Bowman-Birk (soybean) inhibitor, Kunitz pancreatic trypsin inhibitor, porcine Kazal inhibitor, and chicken ovomucoid. The human trypsins demonstrated a higher degree of susceptibility to these inhibitors than did the chymotrypsins while human protease E showed remarkably little inhibition by any of these naturally occurring proteinase inhibitors except for alpha-1-proteinase inhibitor. The contribution of each of these proteolytic enzymes to the total proteolytic activity of crude extracts was also investigated using specific active-site directed reagents. These studies revealed that the trypsins constituted approximately 35% of the proteolytic activity while the chymotrypsins represent approximately 32% of the total proteolytic activity. Human protease E and possibly human pancreatic elastase are responsible for approximately 21% of this activity as measured on crude pancreatic extracts.", "contents": "Inhibition spectra of the human pancreatic endopeptidases. The present work describes the effect of seven naturally occurring proteinase inhibitors on the human pancreatic endopeptidases cationic trypsin, anionic trypsin, chymotrypsin I, chymotrypsin II, and protease E (an elastase-like protease). The inhibitors tested in order of their decreasing effectiveness were alpha-1-proteinase inhibitor (alpha-1-antitrypsin), lima bean trypsin inhibitor, soybean trypsin inhibitor, Bowman-Birk (soybean) inhibitor, Kunitz pancreatic trypsin inhibitor, porcine Kazal inhibitor, and chicken ovomucoid. The human trypsins demonstrated a higher degree of susceptibility to these inhibitors than did the chymotrypsins while human protease E showed remarkably little inhibition by any of these naturally occurring proteinase inhibitors except for alpha-1-proteinase inhibitor. The contribution of each of these proteolytic enzymes to the total proteolytic activity of crude extracts was also investigated using specific active-site directed reagents. These studies revealed that the trypsins constituted approximately 35% of the proteolytic activity while the chymotrypsins represent approximately 32% of the total proteolytic activity. Human protease E and possibly human pancreatic elastase are responsible for approximately 21% of this activity as measured on crude pancreatic extracts."}
{"id": "PMID:1080009", "title": "Partial antibiotic therapy in Haemophilus infuenzae meningitis. Its effect on cerebrospinal fluid abnormalities.", "content": "To examine the influence of partial antibiotic therapy on laboratory findings in Haemophilus influenzae meningitis, 272 untreated cases and 202 partially treated, culture-positive cases from 1953 through 1971 were reviewed. There was no significant difference between the two groups with respect to several cerebrospinal fluid (CSF) values. Both the untreated and partially treated groups had a similar proportion of cases with negative Gram stain and CSF glucose level over 40 mg/100 ml or a predominance of CSF mononuclear cells. Eight patients had received ampicillin sodium, chloramphenicol, or tetracycline for two days or more. The CSF findings of these patients resembled those of the untreated group. The data support the conclusion that antibiotics in the usual outpatient dosages seldom interfere with the diagnosis of H influenzae meningitis.", "contents": "Partial antibiotic therapy in Haemophilus infuenzae meningitis. Its effect on cerebrospinal fluid abnormalities. To examine the influence of partial antibiotic therapy on laboratory findings in Haemophilus influenzae meningitis, 272 untreated cases and 202 partially treated, culture-positive cases from 1953 through 1971 were reviewed. There was no significant difference between the two groups with respect to several cerebrospinal fluid (CSF) values. Both the untreated and partially treated groups had a similar proportion of cases with negative Gram stain and CSF glucose level over 40 mg/100 ml or a predominance of CSF mononuclear cells. Eight patients had received ampicillin sodium, chloramphenicol, or tetracycline for two days or more. The CSF findings of these patients resembled those of the untreated group. The data support the conclusion that antibiotics in the usual outpatient dosages seldom interfere with the diagnosis of H influenzae meningitis."}
{"id": "PMID:1080011", "title": "Clinical experiences with colonoscopy. A review of 100 cases.", "content": "Our experience with 100 colonoscopic examinations are described. The clinical value of the procedure is outlined and the minimal degree of attendant morbidity, when performed by a competent endoscopist, are emphasized. The indications and contraindications for this relatively recently devised diagnostic and therapeutic modality should be commonly known. The procedure should be done whenever indicated.", "contents": "Clinical experiences with colonoscopy. A review of 100 cases. Our experience with 100 colonoscopic examinations are described. The clinical value of the procedure is outlined and the minimal degree of attendant morbidity, when performed by a competent endoscopist, are emphasized. The indications and contraindications for this relatively recently devised diagnostic and therapeutic modality should be commonly known. The procedure should be done whenever indicated."}
{"id": "PMID:1080014", "title": "Increase in IgM-bearing lymphocytes in peripheral blood of pregnant women.", "content": "Lymphocytes from peripheral blood of pregnant women were studied with the use of markers for membrane-bound immunoglobulins (B cells) and receptors for sheep erythrocytes (T cells). It was found that there was a significant increase in the number of IgM-bearing lymphocytes during pregnancy. This returned to normal levels after parturition.", "contents": "Increase in IgM-bearing lymphocytes in peripheral blood of pregnant women. Lymphocytes from peripheral blood of pregnant women were studied with the use of markers for membrane-bound immunoglobulins (B cells) and receptors for sheep erythrocytes (T cells). It was found that there was a significant increase in the number of IgM-bearing lymphocytes during pregnancy. This returned to normal levels after parturition."}
{"id": "PMID:1080012", "title": "Eosinophilic gastroenteritis with malabsorption, extensive villous atrophy, recurrent hemorrhage and chronic pulmonary fibrosis.", "content": "A patient with diffuse eosinophilic gastroenteritis, associated with progressive chronic interstitial pulmonary fibrosis, was followed for 29 years.  His major symptoms were recurrent gastrointestinal bleeding, anemia, diarrhea, malabsorption,  and increasing respiratory insufficiency.  Multiple jejunal biopsies revealed eosinophilic infiltration and extensive villous atrophy.  Results of a lung biopsy and pulmonary function tests were consistent with chronic interstitial pulmonary fibrosis.  His gastrointestinal symptoms responded to steroid therapy, but he died of progressive respiratory insufficiency.  At autopsy, villous atrophy was noted to extend throughout the whole extent of the small intestine in association with multiple \"pseudopolypoidal\" inflammatory lesions.", "contents": "Eosinophilic gastroenteritis with malabsorption, extensive villous atrophy, recurrent hemorrhage and chronic pulmonary fibrosis. A patient with diffuse eosinophilic gastroenteritis, associated with progressive chronic interstitial pulmonary fibrosis, was followed for 29 years.  His major symptoms were recurrent gastrointestinal bleeding, anemia, diarrhea, malabsorption,  and increasing respiratory insufficiency.  Multiple jejunal biopsies revealed eosinophilic infiltration and extensive villous atrophy.  Results of a lung biopsy and pulmonary function tests were consistent with chronic interstitial pulmonary fibrosis.  His gastrointestinal symptoms responded to steroid therapy, but he died of progressive respiratory insufficiency.  At autopsy, villous atrophy was noted to extend throughout the whole extent of the small intestine in association with multiple \"pseudopolypoidal\" inflammatory lesions."}
{"id": "PMID:1080015", "title": "Contractures in partially depolarized muscle treated with caffeine or nitrate.", "content": "Frog toe muscles partially depolarized with a subcontracture concentration (15 or 20mM) of K+ rapidly developed tension when exposed to 1 mM caffeine, which alone did not cause contracture. This action resulted from a lowering of the mechanical threshold by the caffeine and thus was similar to that caused by replacement of Cl- with NO3-. Maximal tension was the same in the caffeine and NO3- media but took much longer to develop in the former. Equilibrating the muscles with caffeine for 60 s (the time calculated for complete diffusion) did not reduce contraction time significantly. However, prolonging the exposure of muscles to caffeine beyond this time enhanced the drug's capacity to potentiate both the twitch and 30 mM K+ contractures and shortened the contraction time of the contractures. Toe muscles that had undergone a contracture in 100 mM K+ and were recovering in 15 mM K+-Ringer generated tension in the presence of 1 mM caffeine or NO3- but only after enough repolarization had been achieved so that a response to a second challenge with 100 mM K+ could be demonstrated. Although prolonged immersion in the K+-enriched recovery solution caused a disappearance of sensitivity to a test depolarization, addition of 1 mM caffeine or replacement of Cl- with NO3- promptly evoked a contracture. These results imply a reversible step in the recovery process that is both potential and time dependent. Since application of 5 mM caffeine to either normally polarized or depolarized muscles always immediately causes contracture, more than one site of action for caffeine is indicated. After disruption of the transverse tubules, partially depolarized muscles showed no response to NO3- or 1 mM caffeine but contracted vigorously when exposed to 5 mM caffeine.", "contents": "Contractures in partially depolarized muscle treated with caffeine or nitrate. Frog toe muscles partially depolarized with a subcontracture concentration (15 or 20mM) of K+ rapidly developed tension when exposed to 1 mM caffeine, which alone did not cause contracture. This action resulted from a lowering of the mechanical threshold by the caffeine and thus was similar to that caused by replacement of Cl- with NO3-. Maximal tension was the same in the caffeine and NO3- media but took much longer to develop in the former. Equilibrating the muscles with caffeine for 60 s (the time calculated for complete diffusion) did not reduce contraction time significantly. However, prolonging the exposure of muscles to caffeine beyond this time enhanced the drug's capacity to potentiate both the twitch and 30 mM K+ contractures and shortened the contraction time of the contractures. Toe muscles that had undergone a contracture in 100 mM K+ and were recovering in 15 mM K+-Ringer generated tension in the presence of 1 mM caffeine or NO3- but only after enough repolarization had been achieved so that a response to a second challenge with 100 mM K+ could be demonstrated. Although prolonged immersion in the K+-enriched recovery solution caused a disappearance of sensitivity to a test depolarization, addition of 1 mM caffeine or replacement of Cl- with NO3- promptly evoked a contracture. These results imply a reversible step in the recovery process that is both potential and time dependent. Since application of 5 mM caffeine to either normally polarized or depolarized muscles always immediately causes contracture, more than one site of action for caffeine is indicated. After disruption of the transverse tubules, partially depolarized muscles showed no response to NO3- or 1 mM caffeine but contracted vigorously when exposed to 5 mM caffeine."}
{"id": "PMID:1080016", "title": "Acid-induced excitation of afferent cardiac sympathetic nerve fibers.", "content": "The effect of acids on activity of afferent sympathetic nerve fibers from the left ventricle has been examined. Action potentials were derived from the upper thoracic communicating rami of the left side of anesthetized dogs. Application of a solution of lactic acid to the left ventricular surface caused excitation in both myelinated and unmyelinated fibers. The minimum concentration required for excitation was 7.5-75 mug/ml for the unmyelinated fibers and 375-750 mug/ml for the myelinated fibers. Excitation of the unmyelinated fibers induced by coronary occlusion was suppressed by pretreatment with sodium bicarbonate, 500 mg/kg. However, excitation of the myelinated fibers was influenced little by the agent. Pretreatment with a large dose of Trasylol failed to suppress excitation induced by coronary occlusion. The result suggests that acidosis plays a role in excitation of the unmyelinated fibers induced by myocardial ischemia, but not in excitation of the myelinated fibers.", "contents": "Acid-induced excitation of afferent cardiac sympathetic nerve fibers. The effect of acids on activity of afferent sympathetic nerve fibers from the left ventricle has been examined. Action potentials were derived from the upper thoracic communicating rami of the left side of anesthetized dogs. Application of a solution of lactic acid to the left ventricular surface caused excitation in both myelinated and unmyelinated fibers. The minimum concentration required for excitation was 7.5-75 mug/ml for the unmyelinated fibers and 375-750 mug/ml for the myelinated fibers. Excitation of the unmyelinated fibers induced by coronary occlusion was suppressed by pretreatment with sodium bicarbonate, 500 mg/kg. However, excitation of the myelinated fibers was influenced little by the agent. Pretreatment with a large dose of Trasylol failed to suppress excitation induced by coronary occlusion. The result suggests that acidosis plays a role in excitation of the unmyelinated fibers induced by myocardial ischemia, but not in excitation of the myelinated fibers."}
{"id": "PMID:1080018", "title": "Freeze-fracture analysis of junctional complexes in human ciliary epithelia.", "content": "The junctions connecting the epithelial cells of the human ciliary body were studied on replicas of freeze-fractured preparations. Gap junctions are present at the lateral surfaces of the cells of the pigmented epithelium. Gap junctions, isolated linear strands and desmosomes are found between the apical surfaces of pigmented and non-pigmented epithelial cells. Gap junctions, zonulae occludentes and desmosomes join the lateral surfaces of the non-pigmented epithelial cells. These findings suggest that the blood-aqueous barrier is located at the level of the zonulae occludentes of the non-pigmented epithelial cells. This is consistent with previous studies using thin sections and tracer substances. The function of the many gap junctions connecting the cells of the two epithelial layers is obscure since their presence is not consistent with electrophysiological data from this region.", "contents": "Freeze-fracture analysis of junctional complexes in human ciliary epithelia. The junctions connecting the epithelial cells of the human ciliary body were studied on replicas of freeze-fractured preparations. Gap junctions are present at the lateral surfaces of the cells of the pigmented epithelium. Gap junctions, isolated linear strands and desmosomes are found between the apical surfaces of pigmented and non-pigmented epithelial cells. Gap junctions, zonulae occludentes and desmosomes join the lateral surfaces of the non-pigmented epithelial cells. These findings suggest that the blood-aqueous barrier is located at the level of the zonulae occludentes of the non-pigmented epithelial cells. This is consistent with previous studies using thin sections and tracer substances. The function of the many gap junctions connecting the cells of the two epithelial layers is obscure since their presence is not consistent with electrophysiological data from this region."}
{"id": "PMID:1080019", "title": "Carbohydrate metabolism of the lens depending on age. V. Problems of mathematical approach and graphical presentation.", "content": "For investigations on age-dependent changes of lens metabolism, longitudinal studies are requisite. Graphic representation of such findings should be based on a proper arithmetic age scale. Using the lens weight for representing the age leads to distortion of the curves, whereby certain periods of life are liable to be overemphasized. In longitudinal studies, data on concentrations should never be given per unit weight, but rather for the whole lens. Absolute values of the whole, intact lens may be referred to a reference value, a percental representation of the curves is possible. With regard to the expectancy of life of test animals one may already state that longitudinal studies including the senile phase will only be feasible with rats.", "contents": "Carbohydrate metabolism of the lens depending on age. V. Problems of mathematical approach and graphical presentation. For investigations on age-dependent changes of lens metabolism, longitudinal studies are requisite. Graphic representation of such findings should be based on a proper arithmetic age scale. Using the lens weight for representing the age leads to distortion of the curves, whereby certain periods of life are liable to be overemphasized. In longitudinal studies, data on concentrations should never be given per unit weight, but rather for the whole lens. Absolute values of the whole, intact lens may be referred to a reference value, a percental representation of the curves is possible. With regard to the expectancy of life of test animals one may already state that longitudinal studies including the senile phase will only be feasible with rats."}
{"id": "PMID:1080020", "title": "[Selective atrophy of the radial peripapillary capillaries and visual field defects in glaucoma (author's transl)].", "content": "The existence and distribution of atrophic radial peripapillary capillaries in 33 autopsy eyes of patients with chronic glaucoma or different optic nerve atrophies are compared with their visual field defects and stage of atrophy or excavation of the disc. No correlation of defective RPC and visual field defects was found. The selective atrophy of RPC is therefore supposed to be secondary to different atrophies of the disc.", "contents": "[Selective atrophy of the radial peripapillary capillaries and visual field defects in glaucoma (author's transl)]. The existence and distribution of atrophic radial peripapillary capillaries in 33 autopsy eyes of patients with chronic glaucoma or different optic nerve atrophies are compared with their visual field defects and stage of atrophy or excavation of the disc. No correlation of defective RPC and visual field defects was found. The selective atrophy of RPC is therefore supposed to be secondary to different atrophies of the disc."}
{"id": "PMID:1080021", "title": "[The course of zonular fiber. A further electronenmicroscopic study (author's transl)].", "content": "Zonular fibers could be shown between the basement membrane of the pigmented epithelium of pars plana and the elastic tissue of Bruch's membrane. A connection of zonular fibrils between the unpigmented epithelium and the basement membrane of pigmented epithelium of the pars plana could not be observed. It is possible to discuss two independent courses of zonular fibers.", "contents": "[The course of zonular fiber. A further electronenmicroscopic study (author's transl)]. Zonular fibers could be shown between the basement membrane of the pigmented epithelium of pars plana and the elastic tissue of Bruch's membrane. A connection of zonular fibrils between the unpigmented epithelium and the basement membrane of pigmented epithelium of the pars plana could not be observed. It is possible to discuss two independent courses of zonular fibers."}
{"id": "PMID:1080022", "title": "[The electro-oculogram in the early postoperative period following fistulizing operations (author's transl)].", "content": "EOGs were obtained from 2 groups of patients suffering from glaucoma chronicum simplex the day before surgery and 3, 5, 8, 11 and 14 days after trephining or fistulizing operation after Scheie. Though and peak potentials of the treated eyes were significantly less than those of the untreated fellow eyes five days after surgery. In addition on the 11. postoperative day after Scheie a second potential decrease was seen. The EOG-changes observed appear to reflect disturbances of the choroidal circulation after fistulizing operations.", "contents": "[The electro-oculogram in the early postoperative period following fistulizing operations (author's transl)]. EOGs were obtained from 2 groups of patients suffering from glaucoma chronicum simplex the day before surgery and 3, 5, 8, 11 and 14 days after trephining or fistulizing operation after Scheie. Though and peak potentials of the treated eyes were significantly less than those of the untreated fellow eyes five days after surgery. In addition on the 11. postoperative day after Scheie a second potential decrease was seen. The EOG-changes observed appear to reflect disturbances of the choroidal circulation after fistulizing operations."}
{"id": "PMID:1080023", "title": "Development of beaks of Rana pipiens larvae.", "content": "Rana pipiens larval beaks of consist of column cells, sheath cells and basal cells which supply cells to column and sheath. Each column consists of disk-like precone cells, cone cells and keratinized cone cells; they are cells in different stages of the process of keratinization. Breaks first appear externally at embryonic stage 24. Epidermal cells align at the tip of the jaw at stage 21. They increase in number and change in shape. Keratinization starts at stage 23. By stage 24, the apical column cells are keratinized and the histological organization is set for the whole larval period. During the larval period, the numbers of column cells increase until stages VIII or IV, stay relatively constant during mid-larval stages, and decrease at late larval stages. The beak is completely shed at stage XX. The widening of the beaks goes on during the entire larval period. Along the cutting edge of the jaw the tooth spikes increase in number and in individual width as the animal grows older and larger. Thyroid hormone causes a premature reduction of the column cell number and a precocious beak loss. The loss of break at metamorphic climax is thyroid dependent event.", "contents": "Development of beaks of Rana pipiens larvae. Rana pipiens larval beaks of consist of column cells, sheath cells and basal cells which supply cells to column and sheath. Each column consists of disk-like precone cells, cone cells and keratinized cone cells; they are cells in different stages of the process of keratinization. Breaks first appear externally at embryonic stage 24. Epidermal cells align at the tip of the jaw at stage 21. They increase in number and change in shape. Keratinization starts at stage 23. By stage 24, the apical column cells are keratinized and the histological organization is set for the whole larval period. During the larval period, the numbers of column cells increase until stages VIII or IV, stay relatively constant during mid-larval stages, and decrease at late larval stages. The beak is completely shed at stage XX. The widening of the beaks goes on during the entire larval period. Along the cutting edge of the jaw the tooth spikes increase in number and in individual width as the animal grows older and larger. Thyroid hormone causes a premature reduction of the column cell number and a precocious beak loss. The loss of break at metamorphic climax is thyroid dependent event."}
{"id": "PMID:1080025", "title": "Postoperative respiratory effects of morphine and halothane anesthesia: a study in patients undergoing cardiac surgery.", "content": "Lung volumes, deadspace, ventilation, and ventilatory response to CO2 challenge were studied on the day before and for the first three days after corrective cardiac surgery. Ten patients underwent coronary artery bypass grafting and ten patients had mitral valve prostheses inserted. Half of the patients in each group received halothane as the major anesthetic, and the other half received morphine sulfate (1-2 mg/kg). Mitral valve-replacement patients anesthetized with morphine showed lower CO2 sensitivity on the first postoperative day than those who received halothane. Patients who had coronary artery bypass grafts tended to hyperventilate during the postoperative period, but this did not occur on the first postoperative day in those who received morphine anesthesia. Respiratory rate was always higher postoperatively, most markedly in patients who receivedhalothane for coronary artery bypass grafts. Vital capacity was diminished by 67 per cent in all groups postoperatively. VD/VT tended to increase during the first and second postoperative days and then decrease toward control values on the third postoperative day in all groups except valve-replacement patients who received morphine. Morphine anesthesia may increase the period of mechanical ventilation necessary after cardiac surgery partly as a result of impaired CO2 sensitivity.", "contents": "Postoperative respiratory effects of morphine and halothane anesthesia: a study in patients undergoing cardiac surgery. Lung volumes, deadspace, ventilation, and ventilatory response to CO2 challenge were studied on the day before and for the first three days after corrective cardiac surgery. Ten patients underwent coronary artery bypass grafting and ten patients had mitral valve prostheses inserted. Half of the patients in each group received halothane as the major anesthetic, and the other half received morphine sulfate (1-2 mg/kg). Mitral valve-replacement patients anesthetized with morphine showed lower CO2 sensitivity on the first postoperative day than those who received halothane. Patients who had coronary artery bypass grafts tended to hyperventilate during the postoperative period, but this did not occur on the first postoperative day in those who received morphine anesthesia. Respiratory rate was always higher postoperatively, most markedly in patients who receivedhalothane for coronary artery bypass grafts. Vital capacity was diminished by 67 per cent in all groups postoperatively. VD/VT tended to increase during the first and second postoperative days and then decrease toward control values on the third postoperative day in all groups except valve-replacement patients who received morphine. Morphine anesthesia may increase the period of mechanical ventilation necessary after cardiac surgery partly as a result of impaired CO2 sensitivity."}
{"id": "PMID:1080026", "title": "Factors of delayed hypersensitity in pulmonary tuberculosis.", "content": "In a study of delayed hypersensitivity in 54 patients with pulmonary tuberculosis who were on specific treatment, 33 patients could not be sensitized to dinitrochlorobenzene (DNCB) although 25 of these were tuberculin (PPD) positive. The majority of the patients also had positive skin reactions to another recall antigen, streptokinase-streptodornase. To exclude a drug effect on DNCB reactivity, 40 untreated patients were studied. Twenty-six (65 per cent) of these did not show DNCB reactivity. The cause for nonsensitization to DNCB was not clear. There was no abnormality in the number of T lymphocytes in the peripheral blood of 28 patients.", "contents": "Factors of delayed hypersensitity in pulmonary tuberculosis. In a study of delayed hypersensitivity in 54 patients with pulmonary tuberculosis who were on specific treatment, 33 patients could not be sensitized to dinitrochlorobenzene (DNCB) although 25 of these were tuberculin (PPD) positive. The majority of the patients also had positive skin reactions to another recall antigen, streptokinase-streptodornase. To exclude a drug effect on DNCB reactivity, 40 untreated patients were studied. Twenty-six (65 per cent) of these did not show DNCB reactivity. The cause for nonsensitization to DNCB was not clear. There was no abnormality in the number of T lymphocytes in the peripheral blood of 28 patients."}
{"id": "PMID:1080024", "title": "Effect of halothane on isometric twitch and tetanus response and the associated heat production in striated muscle of frogs.", "content": "The purpose of these investigations was to determine the effect of halothane on isometric contraction of striated muscle and to measure the associated heat production. This basic information is necessary before studies more directly relating to malignant hyperthermia are undertaken. Sartorius muscles were isolate from Rana pipiens during winter and summer months. It appears from these experiments that there is a prolongation of the relaxation phase of the twitch and tetanus responses with low concentrations of halothane, with a more diffuse effect on the contractile process evident at higher administered concentrations. The results of heat measurements, using a sensitive thermopile-galvanometer system, are compatible with the hypotheses that this effect on relaxation could result from either an interference with calcium reuptake by the sarcoplasmic reticulum or an increased affinity of the troponintropomyosin complex for available calcium.", "contents": "Effect of halothane on isometric twitch and tetanus response and the associated heat production in striated muscle of frogs. The purpose of these investigations was to determine the effect of halothane on isometric contraction of striated muscle and to measure the associated heat production. This basic information is necessary before studies more directly relating to malignant hyperthermia are undertaken. Sartorius muscles were isolate from Rana pipiens during winter and summer months. It appears from these experiments that there is a prolongation of the relaxation phase of the twitch and tetanus responses with low concentrations of halothane, with a more diffuse effect on the contractile process evident at higher administered concentrations. The results of heat measurements, using a sensitive thermopile-galvanometer system, are compatible with the hypotheses that this effect on relaxation could result from either an interference with calcium reuptake by the sarcoplasmic reticulum or an increased affinity of the troponintropomyosin complex for available calcium."}
{"id": "PMID:1080028", "title": "On the \"easy bruising\" syndrome with normal platelet count. A study of 75 patients.", "content": "Seventy-five consecutive patients with normal platelet counts were investigated for \"easy bruising.\" All had a normal coagulation profile, and all except four were women. None were on aspirin or other antiplatelet agents. Two specific groups could be delineated. In type I (44 patients, mean age, 35), platelet function was normal to supranormal. Megathrombocyte number was elevated in 60% of patients and correlated with the presence of antiplatelet antibody in 30% of patients. In type II (31 patients, mean age, 34), platelet function was abnormal: impaired epinephrine aggregation (primary and secondary wave) in 97%, impaired connective tissue aggregation in 77%, and impaired ADP aggregation in 42%. Megathrombocyte number was elevated in 71%, and antiplatelet antibody was present in 38% of patients. The \"easy bruising\" syndrome can be differentiated into two categories: type I, in which a platelet abnormality is unlikely, and type II, in which a platelet abnormality exists. Elevated incidence of antiplatelet antibody in both groups suggests  a possible autoimmune cause.", "contents": "On the \"easy bruising\" syndrome with normal platelet count. A study of 75 patients. Seventy-five consecutive patients with normal platelet counts were investigated for \"easy bruising.\" All had a normal coagulation profile, and all except four were women. None were on aspirin or other antiplatelet agents. Two specific groups could be delineated. In type I (44 patients, mean age, 35), platelet function was normal to supranormal. Megathrombocyte number was elevated in 60% of patients and correlated with the presence of antiplatelet antibody in 30% of patients. In type II (31 patients, mean age, 34), platelet function was abnormal: impaired epinephrine aggregation (primary and secondary wave) in 97%, impaired connective tissue aggregation in 77%, and impaired ADP aggregation in 42%. Megathrombocyte number was elevated in 71%, and antiplatelet antibody was present in 38% of patients. The \"easy bruising\" syndrome can be differentiated into two categories: type I, in which a platelet abnormality is unlikely, and type II, in which a platelet abnormality exists. Elevated incidence of antiplatelet antibody in both groups suggests  a possible autoimmune cause."}
{"id": "PMID:1080031", "title": "Solar retinopathy and foveomacular retinitis.", "content": "The visual disturbances caused by looking into the sun or a solar eclipse have been known for many years. Recently, there have been discussions about whether or not solar retinopathy is a distinct clinical entity or identical to the disease termed foveomacular retinitis. A series of 36 patients has been evaluated at Letterman Army Medical Center with the ophthalmoscopic appearance compatible with both conditions in various stages of the disease. By use of clinical history, funduscopy, visual fields, Amsler Grid, color acuity testing, color fundus photography, and fluorescein dye studies, it is our distinct impression that these two conditions are identical.", "contents": "Solar retinopathy and foveomacular retinitis. The visual disturbances caused by looking into the sun or a solar eclipse have been known for many years. Recently, there have been discussions about whether or not solar retinopathy is a distinct clinical entity or identical to the disease termed foveomacular retinitis. A series of 36 patients has been evaluated at Letterman Army Medical Center with the ophthalmoscopic appearance compatible with both conditions in various stages of the disease. By use of clinical history, funduscopy, visual fields, Amsler Grid, color acuity testing, color fundus photography, and fluorescein dye studies, it is our distinct impression that these two conditions are identical."}
{"id": "PMID:1080034", "title": "Trisomy 4p in a family with A t(4;15).", "content": "This report concerns a trisomy 4p in a mentally retarded girl with multiple congenital anomalies. A translocation between chromsomes Nos. 4 and 15 was discovered in three generations of the patient's pedigree.", "contents": "Trisomy 4p in a family with A t(4;15). This report concerns a trisomy 4p in a mentally retarded girl with multiple congenital anomalies. A translocation between chromsomes Nos. 4 and 15 was discovered in three generations of the patient's pedigree."}
{"id": "PMID:1080035", "title": "[Ultrastructure study on the whole chromosome in radiation induced anomalies: II. Re-arrangements and fragments].", "content": "Chromosome aberrations induced by 500 rads gamma ray irradiation (dicentrics, rings, and fragments) were observed at the ultrastructural level using the \"whole mount\" technique. The area of chromatid rejoining could not be demonstrated. The pairing of fragments observed could be explained by the perisistence of interchromatid fibers.", "contents": "[Ultrastructure study on the whole chromosome in radiation induced anomalies: II. Re-arrangements and fragments]. Chromosome aberrations induced by 500 rads gamma ray irradiation (dicentrics, rings, and fragments) were observed at the ultrastructural level using the \"whole mount\" technique. The area of chromatid rejoining could not be demonstrated. The pairing of fragments observed could be explained by the perisistence of interchromatid fibers."}
{"id": "PMID:1080036", "title": "[Partial 14q trisomy. I. Partial 14q trisomy by maternal translocation t(10;14) (p15.2;q22)].", "content": "A balanced reciprocal translocation t(10;14)(p15.2;q22) is present in the mother and the maternal grand mother of two boys. One, with a normal phenotype, received the balanced translocation, the other, exhibiting congenital malformations, is trisomic for the proximal part of 14q.", "contents": "[Partial 14q trisomy. I. Partial 14q trisomy by maternal translocation t(10;14) (p15.2;q22)]. A balanced reciprocal translocation t(10;14)(p15.2;q22) is present in the mother and the maternal grand mother of two boys. One, with a normal phenotype, received the balanced translocation, the other, exhibiting congenital malformations, is trisomic for the proximal part of 14q."}
{"id": "PMID:1080037", "title": "[Partial trisomy 14q II.--Partial trisomy 14q due to a maternal t(12; 14) (q24.4; q21)].", "content": "The phenotype of an 18-month-old male infant trisomic for the proximal portion of the long arm of chromosome 14 was reported and compared with that of previously reported cases. For the identification of the resulting syndrome, the most consistent features are psychomotor and growth retardation, and an oval, dysmorphic facies which includes a distinctive form of the mouth and a prominent nose. The trisomy in the child reported here is due to a familial translocation transmitted by the mother and present in at least three generations: t(12;14)(q24.4;q21). The 12q duplication in the child's genome is minimal and does not seem to have contributed to his phenotype.", "contents": "[Partial trisomy 14q II.--Partial trisomy 14q due to a maternal t(12; 14) (q24.4; q21)]. The phenotype of an 18-month-old male infant trisomic for the proximal portion of the long arm of chromosome 14 was reported and compared with that of previously reported cases. For the identification of the resulting syndrome, the most consistent features are psychomotor and growth retardation, and an oval, dysmorphic facies which includes a distinctive form of the mouth and a prominent nose. The trisomy in the child reported here is due to a familial translocation transmitted by the mother and present in at least three generations: t(12;14)(q24.4;q21). The 12q duplication in the child's genome is minimal and does not seem to have contributed to his phenotype."}
{"id": "PMID:1080038", "title": "Trisomy iop. A report of two cases due to a familial translocation rcp (10;21) (pII;pII).", "content": "Trisomy for the short arm of chromosome number 10 was diagnosed (by a G-banding method) in two sisters with multiple congenital defects. Their mother and two other sisters showed a balanced translocation 46,XX rcp(10;21)(p11;p11), so the affected girls were the result of a maternal adjacent-1 meiotic segregation with a karyotype 46,XX, der(21), rcp(10;21)(p11;p11)mat. The concordant features in the abnormal patients constitute the following syndrome: severe psychomotor retardation, congenital microsomatia, mild hydrocephalus with cranium-face disproportion, low set ears with hypoplastic helix, ocular colobomata, pulmonary stenosis,flexion deformity of wrists and elbows, bilateral fifth finger clinodactyly and simian creases, hypoplastic dermal ridges, bilateral talipes, persistent icterus and delayed bone age. The phenotypical and cytogenetic findings permit the individualization of the 10p trisomy.", "contents": "Trisomy iop. A report of two cases due to a familial translocation rcp (10;21) (pII;pII). Trisomy for the short arm of chromosome number 10 was diagnosed (by a G-banding method) in two sisters with multiple congenital defects. Their mother and two other sisters showed a balanced translocation 46,XX rcp(10;21)(p11;p11), so the affected girls were the result of a maternal adjacent-1 meiotic segregation with a karyotype 46,XX, der(21), rcp(10;21)(p11;p11)mat. The concordant features in the abnormal patients constitute the following syndrome: severe psychomotor retardation, congenital microsomatia, mild hydrocephalus with cranium-face disproportion, low set ears with hypoplastic helix, ocular colobomata, pulmonary stenosis,flexion deformity of wrists and elbows, bilateral fifth finger clinodactyly and simian creases, hypoplastic dermal ridges, bilateral talipes, persistent icterus and delayed bone age. The phenotypical and cytogenetic findings permit the individualization of the 10p trisomy."}
{"id": "PMID:1080039", "title": "[Interstitial deletion of the long arm of one 11 chromosome].", "content": "A case of in testitial deletion of the long arm of chromosome 11 associated with mild facial deformity, broad skull and mental retardation is reported.", "contents": "[Interstitial deletion of the long arm of one 11 chromosome]. A case of in testitial deletion of the long arm of chromosome 11 associated with mild facial deformity, broad skull and mental retardation is reported."}
{"id": "PMID:1080040", "title": "[Partial 7q trisomy].", "content": "A 17-month old boy is partially trisomic for 7q22 and 7q31 due to a probable insertion in the paternal chromosome 13. The phenotype of the patient is similar to that of two other patients reported in the literature.", "contents": "[Partial 7q trisomy]. A 17-month old boy is partially trisomic for 7q22 and 7q31 due to a probable insertion in the paternal chromosome 13. The phenotype of the patient is similar to that of two other patients reported in the literature."}
{"id": "PMID:1080041", "title": "[Mosaic 14 trisomy in a female child with multiple abnormalities].", "content": "Chromosomal preparations obtained from lymphocytes and fibroblasts revealed a minor clone, 47,XX,+14, in a female newborn with multiple malformations. Other types of rearrangement of chromosome 14 are discussed.", "contents": "[Mosaic 14 trisomy in a female child with multiple abnormalities]. Chromosomal preparations obtained from lymphocytes and fibroblasts revealed a minor clone, 47,XX,+14, in a female newborn with multiple malformations. Other types of rearrangement of chromosome 14 are discussed."}
{"id": "PMID:1080042", "title": "Centrally-mediated cardiovascular effects of 5-hydroxytryptophan in MAO-inhibited dogs: modification by autonomic antagonists.", "content": "In MAO-inhibited dogs, 5-hydroxytryptophan (5-HTP), either 5 or 10 mg/kg i.v., caused hypotension with variable effects on heart rate. Reflex responses to bilateral carotid artery occlusion (BCO) were greatly inhibited by 5-HTP. The hypotensive effects were markedly inhibited by cerebral and extracerebral decarboxylase inhibition with RO 4-4602 and the inhibition of BCO was delayed. Selective extracerebral decarboxylase inhibitation with MK 486 did not prevent either the hypotensive action of 5-HTP or the effect on BCO, although the amino acid now consistently caused bradycardia. Left ventricular pressure and dP/dt were reduced, but cardiac output was maintained by an increase in stroke volume. Hypotension was due predominantly to decreased peripheral resistance. The hypotensive action of 5-HTP was abolished or greatly attenuated after pretreatment with either yohimbine or methysergide, but was unaffected by haloperidol. These results indicate that cerebral decarboxylation and formation of 5-HT are responsible for the hypotension after 5-HTP in MAO inhibited dogs.", "contents": "Centrally-mediated cardiovascular effects of 5-hydroxytryptophan in MAO-inhibited dogs: modification by autonomic antagonists. In MAO-inhibited dogs, 5-hydroxytryptophan (5-HTP), either 5 or 10 mg/kg i.v., caused hypotension with variable effects on heart rate. Reflex responses to bilateral carotid artery occlusion (BCO) were greatly inhibited by 5-HTP. The hypotensive effects were markedly inhibited by cerebral and extracerebral decarboxylase inhibition with RO 4-4602 and the inhibition of BCO was delayed. Selective extracerebral decarboxylase inhibitation with MK 486 did not prevent either the hypotensive action of 5-HTP or the effect on BCO, although the amino acid now consistently caused bradycardia. Left ventricular pressure and dP/dt were reduced, but cardiac output was maintained by an increase in stroke volume. Hypotension was due predominantly to decreased peripheral resistance. The hypotensive action of 5-HTP was abolished or greatly attenuated after pretreatment with either yohimbine or methysergide, but was unaffected by haloperidol. These results indicate that cerebral decarboxylation and formation of 5-HT are responsible for the hypotension after 5-HTP in MAO inhibited dogs."}
{"id": "PMID:1080043", "title": "Regulation of C3-enzymes in facultative phototrophic bacteria: the cold-labile pyruvate kinase of Rhodopseudomonas sphaeroides.", "content": "Pyruvate kinase (EC2.7.1.40) from Rhodopseudomonas sphaeroides was purified 40-fold be precipitation with protamine sulfate and ammonium sulfate followed by gel-filtration. The preparations obtained from cells grown with different carbon sources or cultural conditions differ with respect to specific activity but not with respect to molecular weight (250000 dalton) or regulatory properties. The phosphoenolpyruvate (PEP)-saturation cruve of the enzyme is sigmoidal with Hill coefficients varying from nH equals 1.8 (pH 9.2) to 2.7 (pH 6.0). The enzyme is activated by adenosinemonophosphate (AMP) and the sugarmonophosphates ribose-5-phosphate (R-5-P), glucose-6-phosphate (G-6-P), and-to a lesser extent-fructose-6-phosphate (F-6-P). Fructose-1.6-bisphosphate (FDP) has no measurable effect. Inhibitors of the enzyme are adenosinetriphosphate (ATP), inorganic phosphate (Pi) and the dicarboxylic acids succinate and fumarate. Kinetic analysis reveals that the sugar-phosphates and the dicarboxylic acids act as true allosteric ligands, whereas the effects of AMP, ATP, and Pi cannot be interpreted soley in terms of allosteric interactions. Cold-treatment of the enzymes lead to a rapid loss of activity, but does not change the regulatory properties of the enzyme. Analysis of the kinetics of cold-inactivation and its reversal at 30 percent C, together with studies on the gelfiltration behaviour of the native and the cold-treated enzyme make it likely that the cold-induced loss of activity is due to a dissociation of the enzyme.", "contents": "Regulation of C3-enzymes in facultative phototrophic bacteria: the cold-labile pyruvate kinase of Rhodopseudomonas sphaeroides. Pyruvate kinase (EC2.7.1.40) from Rhodopseudomonas sphaeroides was purified 40-fold be precipitation with protamine sulfate and ammonium sulfate followed by gel-filtration. The preparations obtained from cells grown with different carbon sources or cultural conditions differ with respect to specific activity but not with respect to molecular weight (250000 dalton) or regulatory properties. The phosphoenolpyruvate (PEP)-saturation cruve of the enzyme is sigmoidal with Hill coefficients varying from nH equals 1.8 (pH 9.2) to 2.7 (pH 6.0). The enzyme is activated by adenosinemonophosphate (AMP) and the sugarmonophosphates ribose-5-phosphate (R-5-P), glucose-6-phosphate (G-6-P), and-to a lesser extent-fructose-6-phosphate (F-6-P). Fructose-1.6-bisphosphate (FDP) has no measurable effect. Inhibitors of the enzyme are adenosinetriphosphate (ATP), inorganic phosphate (Pi) and the dicarboxylic acids succinate and fumarate. Kinetic analysis reveals that the sugar-phosphates and the dicarboxylic acids act as true allosteric ligands, whereas the effects of AMP, ATP, and Pi cannot be interpreted soley in terms of allosteric interactions. Cold-treatment of the enzymes lead to a rapid loss of activity, but does not change the regulatory properties of the enzyme. Analysis of the kinetics of cold-inactivation and its reversal at 30 percent C, together with studies on the gelfiltration behaviour of the native and the cold-treated enzyme make it likely that the cold-induced loss of activity is due to a dissociation of the enzyme."}
{"id": "PMID:1080044", "title": "[Percutaneous stimulation of sensitive peripheral nerves in acute crises of essential trigeminal neuralgia].", "content": "The electric stimulation of sensitive peripheric fibres has been used with good results in the control of pain. It is belived that the modern theory of pain phisiology offers scientific basis to explain its action mechanism. This method was applied to twenty-one patients suffering from trigeminalgia, being continually stimulated during thirty minutes with variable voltages, according to the affected region. As a result, it was obtained the immediate relief of pain for periods of time varying from six and forty-eight hours.", "contents": "[Percutaneous stimulation of sensitive peripheral nerves in acute crises of essential trigeminal neuralgia]. The electric stimulation of sensitive peripheric fibres has been used with good results in the control of pain. It is belived that the modern theory of pain phisiology offers scientific basis to explain its action mechanism. This method was applied to twenty-one patients suffering from trigeminalgia, being continually stimulated during thirty minutes with variable voltages, according to the affected region. As a result, it was obtained the immediate relief of pain for periods of time varying from six and forty-eight hours."}
{"id": "PMID:1080045", "title": "[Hereditary multiple exostosis with spinal cord compression].", "content": "A case of hereditary multiple exostoses successfully operated is reported. The patient, a 15 year-old white brazilian boy, was admitted with tetraplegia and Babinski's sign. Early diagnosis followed by prompt surgery may prevent permanent spinal cord damage.", "contents": "[Hereditary multiple exostosis with spinal cord compression]. A case of hereditary multiple exostoses successfully operated is reported. The patient, a 15 year-old white brazilian boy, was admitted with tetraplegia and Babinski's sign. Early diagnosis followed by prompt surgery may prevent permanent spinal cord damage."}
{"id": "PMID:1080046", "title": "Anesthesia of the ear by iontophoresis of lidocaine.", "content": "Anesthesia of the tympanic membrane and ear canal by the use of iontophoresis of local anesthetics is a painless and safe method of anesthesia for office myringotomy and polyethylene tube insertion, and possibly for surgical use as well.  With the use of guinea pig models, the introduction of 4% lidocaine hydrochloride solution in the middle ear space with iontophoresis revealed no toxic effects on the eighth nerve, and epinephrine hydrocloride potentiates the effects of lidocaine and has no protective mechanism on the eighth nerve function.", "contents": "Anesthesia of the ear by iontophoresis of lidocaine. Anesthesia of the tympanic membrane and ear canal by the use of iontophoresis of local anesthetics is a painless and safe method of anesthesia for office myringotomy and polyethylene tube insertion, and possibly for surgical use as well.  With the use of guinea pig models, the introduction of 4% lidocaine hydrochloride solution in the middle ear space with iontophoresis revealed no toxic effects on the eighth nerve, and epinephrine hydrocloride potentiates the effects of lidocaine and has no protective mechanism on the eighth nerve function."}
{"id": "PMID:1080047", "title": "Trisomy 18.  A temporal bone report.", "content": "Since Edwards first described the trisomy 17-18 syndrome in 1960, the findings in the temporal bones of only four patients with this condition have been reported.  They varied widely, ranging from a normal temporal bone, to severe malformations of both the middle and inner ear structures.  This study describes the temporal bone findings in a patient with this syndrome.  Many of the abnormalities described previously were present as well as the following unreported findings: complete bony atresia of the external canal, an aberrant tensor tympani muscle that did not insert into the malleus but rather attached to a dehiscent area in the Fallopian canal, wide short utricular and saccular ducts, and a widely patent cochlear aqueduct.  There was also a developmental arrest of the membranous structures within the cochlea that probably occurred during the 12th week of fetal life.", "contents": "Trisomy 18.  A temporal bone report. Since Edwards first described the trisomy 17-18 syndrome in 1960, the findings in the temporal bones of only four patients with this condition have been reported.  They varied widely, ranging from a normal temporal bone, to severe malformations of both the middle and inner ear structures.  This study describes the temporal bone findings in a patient with this syndrome.  Many of the abnormalities described previously were present as well as the following unreported findings: complete bony atresia of the external canal, an aberrant tensor tympani muscle that did not insert into the malleus but rather attached to a dehiscent area in the Fallopian canal, wide short utricular and saccular ducts, and a widely patent cochlear aqueduct.  There was also a developmental arrest of the membranous structures within the cochlea that probably occurred during the 12th week of fetal life."}
{"id": "PMID:1080048", "title": "Incidence of vestibular symptomatology in 2,500 U.S. Navy diving accidents (1933-1970).", "content": "The U.S. Navy diving accident records of 2,500 cases for the years 1933 to 1970 were analyzed and sorted into Type I and Type II decompression sickness. Type II was further sorted into \"vestibular\" and \"other\" categories. It was concluded that Type II symptoms accounted for 30% of the decompression accidents and it was estimated that the overall incidence of vestibular symptomatology was between 10 and 20%. Nearly 30% of the Type II cases were diagnosed as having vestibular involvement, although almost 60% of the cases contained a report of a symptom typically associated with the vestibular system complex, e.g., dizziness and nausea.", "contents": "Incidence of vestibular symptomatology in 2,500 U.S. Navy diving accidents (1933-1970). The U.S. Navy diving accident records of 2,500 cases for the years 1933 to 1970 were analyzed and sorted into Type I and Type II decompression sickness. Type II was further sorted into \"vestibular\" and \"other\" categories. It was concluded that Type II symptoms accounted for 30% of the decompression accidents and it was estimated that the overall incidence of vestibular symptomatology was between 10 and 20%. Nearly 30% of the Type II cases were diagnosed as having vestibular involvement, although almost 60% of the cases contained a report of a symptom typically associated with the vestibular system complex, e.g., dizziness and nausea."}
{"id": "PMID:1080049", "title": "Antihypertensive drug therapy in USAF flying personnel.", "content": "The reviewed data concerns hypertensive USAF aircrew members, as available from the WAVR File (July 1973) and the records of the Aeromedical Consultation Service at USAFSAM (1 July 1972-30 June 1973). Of over 6,500 entries in the WAVR File, hypertension was listed as a diagnosis for 754 individuals. Drug therapy had been instituted in 379 cases (50.3%) of the total. Statistics regarding ranks, age groups, and aeronautical ratings are presented. The group included 520 pilots, of whom 268 were receiving drug therapy. Associated medical conditions, e.g., abnormal EKG findings, carbohydrate intolerance, and hyperuriciemia, are presented. Similar data are reviewed for the 84 patients evaluated on the Aeromedical Consultation Service (USAFSAM).", "contents": "Antihypertensive drug therapy in USAF flying personnel. The reviewed data concerns hypertensive USAF aircrew members, as available from the WAVR File (July 1973) and the records of the Aeromedical Consultation Service at USAFSAM (1 July 1972-30 June 1973). Of over 6,500 entries in the WAVR File, hypertension was listed as a diagnosis for 754 individuals. Drug therapy had been instituted in 379 cases (50.3%) of the total. Statistics regarding ranks, age groups, and aeronautical ratings are presented. The group included 520 pilots, of whom 268 were receiving drug therapy. Associated medical conditions, e.g., abnormal EKG findings, carbohydrate intolerance, and hyperuriciemia, are presented. Similar data are reviewed for the 84 patients evaluated on the Aeromedical Consultation Service (USAFSAM)."}
{"id": "PMID:1080051", "title": "[Effect of the protease inhibitor aprotinin on pulmonary function and on the inhibitory activity of sputum in patients with chronic obstructive bronchitis].", "content": "It has been investigated whether a substitution of protease inhibitor deficiency is indicated in case of chronic obstructive airway disease. As a therapeutic possibility, apronitin isolated from bovine organs (tyasylol), which in vitro inhibits sputum proteases up to 80 per cent was tested. Besides infusion, inhalation was chosen for application by which a protease inhibition could be attained. We observed an inhibition of the course of illness associated with a good tolerance of the preparation. Whether a therapy applying the addition of protease inhibitor is reasonable in the long run in chronic diseases cannot yet be concluded from these investigations.", "contents": "[Effect of the protease inhibitor aprotinin on pulmonary function and on the inhibitory activity of sputum in patients with chronic obstructive bronchitis]. It has been investigated whether a substitution of protease inhibitor deficiency is indicated in case of chronic obstructive airway disease. As a therapeutic possibility, apronitin isolated from bovine organs (tyasylol), which in vitro inhibits sputum proteases up to 80 per cent was tested. Besides infusion, inhalation was chosen for application by which a protease inhibition could be attained. We observed an inhibition of the course of illness associated with a good tolerance of the preparation. Whether a therapy applying the addition of protease inhibitor is reasonable in the long run in chronic diseases cannot yet be concluded from these investigations."}
{"id": "PMID:1080053", "title": "A possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of Rhodopseudomonas sphaeroides.", "content": "A previously uncharacterized bacteriochlorophyll-less mutant (mutant 8) of Rhodopseudomonas sphaeroides has been found to excrete a tetrapyrrole-protein complex into the incubation medium. The structure of the major pigment of the complex was characterized as 2-desacetyl-2-vinylbacteriopheophorbide. The corresponding magnesium derivative does not fit into the currently proposed biosynthetic pathway for bacteriochlorophyll, and thus may indicate the existence of an alternate pathway of bacteriochlorophyll synthesis in this organism. Such an alternate pathway would be possible if reduction from the chlorin to the tetrahydroporphyrin stage can occur either before or after hydration of the 2-vinyl substituent of chlorophyllide a to an alpha-hydroxyethyl group.", "contents": "A possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of Rhodopseudomonas sphaeroides. A previously uncharacterized bacteriochlorophyll-less mutant (mutant 8) of Rhodopseudomonas sphaeroides has been found to excrete a tetrapyrrole-protein complex into the incubation medium. The structure of the major pigment of the complex was characterized as 2-desacetyl-2-vinylbacteriopheophorbide. The corresponding magnesium derivative does not fit into the currently proposed biosynthetic pathway for bacteriochlorophyll, and thus may indicate the existence of an alternate pathway of bacteriochlorophyll synthesis in this organism. Such an alternate pathway would be possible if reduction from the chlorin to the tetrahydroporphyrin stage can occur either before or after hydration of the 2-vinyl substituent of chlorophyllide a to an alpha-hydroxyethyl group."}
{"id": "PMID:1080054", "title": "Effect of strong illumination on the ion efflux from the isolated discs of frog photoreceptors.", "content": "(1) Low levels of illumination do not modify the efflux of the radioisotopes 22Na, 86Rb, 36Cl, and 45Ca from the isolated discs of the photoreceptors of the frog (Rana Catesbeiana). (2) The effluxes of 22Na+, 86Rb+ and 36Cl- increase when the discs are illuminated with more than 10(4) erg/cm2 per s for a few minutes. There is no effect on the efflux of 45Ca2+ or of [14C]urea. (3) The effect is greater for monochromatic lights of wavelengths in the shorter region of the spectrum. (4) The effect is also present in bleached visual membranes.", "contents": "Effect of strong illumination on the ion efflux from the isolated discs of frog photoreceptors. (1) Low levels of illumination do not modify the efflux of the radioisotopes 22Na, 86Rb, 36Cl, and 45Ca from the isolated discs of the photoreceptors of the frog (Rana Catesbeiana). (2) The effluxes of 22Na+, 86Rb+ and 36Cl- increase when the discs are illuminated with more than 10(4) erg/cm2 per s for a few minutes. There is no effect on the efflux of 45Ca2+ or of [14C]urea. (3) The effect is greater for monochromatic lights of wavelengths in the shorter region of the spectrum. (4) The effect is also present in bleached visual membranes."}
{"id": "PMID:1080055", "title": "Osmotically induced electrical changes in isolated bullfrog small intestine.", "content": "1. Steady state values of cell water, intracellular Na+ and K+ concentrations, and the electrical parameters ETr, Em, and Isc in the mucosa of isolated bullfrog small intestine were determined following immersion in sodium sulfate Ringer solutions with identical ionic composition but different osmolalities. 2. Cell water and intracellular K+ concentration were inversely related to the osmolality of the bathing medium. During 1 h immersion, intracellular Na+ concentration was not significantly affected by an increase or decrease in external osmolality. 3. Replacement of a hypotonic or an approximately isotonic (normal) medium by a medium of greater osmolality caused statistically significant decreases in ETr, Isc and the (inside negative) magnitude of Em. Conversely, when a hypertonic or a normal medium was replaced by one of lower osmolality, significant increases in the magnitude of these parameters were observed. 4. An equivalent circuit model for the epithelial cell layer, in which the resistance of a relatively highly conducting extracellular shunt pathway is assumed to be the major determinant of the electrical responses of the small intestine to external osmolality, has been shown to account satisfactorily for the observed changes in ETr and Em. In terms of this model, the experimentally observed dependence of Isc on external osmolality requires that, even when both the mucosal and the serosal sides of the tissue are bathed by identical media, isolated bullfrog small intestine maintains a finite diffusion potential across the shunt pathway. This is consistent with current views concerning transepithelial ionic transfer mechanisms.", "contents": "Osmotically induced electrical changes in isolated bullfrog small intestine. 1. Steady state values of cell water, intracellular Na+ and K+ concentrations, and the electrical parameters ETr, Em, and Isc in the mucosa of isolated bullfrog small intestine were determined following immersion in sodium sulfate Ringer solutions with identical ionic composition but different osmolalities. 2. Cell water and intracellular K+ concentration were inversely related to the osmolality of the bathing medium. During 1 h immersion, intracellular Na+ concentration was not significantly affected by an increase or decrease in external osmolality. 3. Replacement of a hypotonic or an approximately isotonic (normal) medium by a medium of greater osmolality caused statistically significant decreases in ETr, Isc and the (inside negative) magnitude of Em. Conversely, when a hypertonic or a normal medium was replaced by one of lower osmolality, significant increases in the magnitude of these parameters were observed. 4. An equivalent circuit model for the epithelial cell layer, in which the resistance of a relatively highly conducting extracellular shunt pathway is assumed to be the major determinant of the electrical responses of the small intestine to external osmolality, has been shown to account satisfactorily for the observed changes in ETr and Em. In terms of this model, the experimentally observed dependence of Isc on external osmolality requires that, even when both the mucosal and the serosal sides of the tissue are bathed by identical media, isolated bullfrog small intestine maintains a finite diffusion potential across the shunt pathway. This is consistent with current views concerning transepithelial ionic transfer mechanisms."}
{"id": "PMID:1080058", "title": "B lymphocytes in primary and secondary deficiencies of humoral immunity.", "content": "The quantitative studies of B lymphocytes in peripheral blood have been performed in various forms of primary and secondary immunodeficiency disease in man. X-linked agammaglobulinemia was found to comprise two sub-types, one lacking B-cell population, the other showing low numbers of B lymphocytes. The absence of B cells in severe combined immunodeficiency was corrected by marrow transplants in 3 children. Cases of DiGeorge syndrome and lepromatous leprosy showed an absolute increase in numbers of B lymphocytes in peripheral blood, probably a compensatory mechanism in the market deficit of T-cell population and function. The reconstitution of DiGeorge syndrome by fetal thymus transplant reversed the abnormally high percentage of B lymphocytes.", "contents": "B lymphocytes in primary and secondary deficiencies of humoral immunity. The quantitative studies of B lymphocytes in peripheral blood have been performed in various forms of primary and secondary immunodeficiency disease in man. X-linked agammaglobulinemia was found to comprise two sub-types, one lacking B-cell population, the other showing low numbers of B lymphocytes. The absence of B cells in severe combined immunodeficiency was corrected by marrow transplants in 3 children. Cases of DiGeorge syndrome and lepromatous leprosy showed an absolute increase in numbers of B lymphocytes in peripheral blood, probably a compensatory mechanism in the market deficit of T-cell population and function. The reconstitution of DiGeorge syndrome by fetal thymus transplant reversed the abnormally high percentage of B lymphocytes."}
{"id": "PMID:1080056", "title": "[Photochromism of visual pigments. I. Formation of isochrome products during reversible transformations of frog rhodopsin].", "content": "Reversible phototransformations of rhodopsin of the frog in vitro (in digitonine extracts) at -22 degrees C under the effect of light with gamma 579 and 435 nm are reversible processes. However, while heating photogeneration product, besides true rhodopsin, thermo-unstable form is (pseudorhodopsin) is revealed. It owes its name due to the similarity of its spectral properties and photosensitivity with rhodopsin. At temperatures higher than -22 degrees C the reversibility of the system is disturbed. The nature of pseudorhodopsin is discussed.", "contents": "[Photochromism of visual pigments. I. Formation of isochrome products during reversible transformations of frog rhodopsin]. Reversible phototransformations of rhodopsin of the frog in vitro (in digitonine extracts) at -22 degrees C under the effect of light with gamma 579 and 435 nm are reversible processes. However, while heating photogeneration product, besides true rhodopsin, thermo-unstable form is (pseudorhodopsin) is revealed. It owes its name due to the similarity of its spectral properties and photosensitivity with rhodopsin. At temperatures higher than -22 degrees C the reversibility of the system is disturbed. The nature of pseudorhodopsin is discussed."}
{"id": "PMID:1080059", "title": "Thymus transplants: a look to the future.", "content": "In a review of thymus transplants performed in a variety of immunodeficiency syndromes, results in the DiGeorge syndrome seen uniformly successful. Rapid restoration of lymphocyte responsibility in vitro and achievement of normal peripheral blood lymphocyte counts are characteristic. In nearly all other types of isolated thymic deficiency or combined immunodeficiency there has been only transient or meager restitution and more often than not complete failure. A few exceptions were noted in a combined immunodeficiency state and in two cases of isolated T-cell deficiency. The use of transfer factor may have played an important adjunctive role. Future attempts must depend upon more precise characterization of the defects, utilizing attempts to define subpopulations of T and B cells; consideration of HL-A restrictions; methods of implantation and use of adjunctive measures (eg thymosin) or other cell populations (such as liver or spleen).", "contents": "Thymus transplants: a look to the future. In a review of thymus transplants performed in a variety of immunodeficiency syndromes, results in the DiGeorge syndrome seen uniformly successful. Rapid restoration of lymphocyte responsibility in vitro and achievement of normal peripheral blood lymphocyte counts are characteristic. In nearly all other types of isolated thymic deficiency or combined immunodeficiency there has been only transient or meager restitution and more often than not complete failure. A few exceptions were noted in a combined immunodeficiency state and in two cases of isolated T-cell deficiency. The use of transfer factor may have played an important adjunctive role. Future attempts must depend upon more precise characterization of the defects, utilizing attempts to define subpopulations of T and B cells; consideration of HL-A restrictions; methods of implantation and use of adjunctive measures (eg thymosin) or other cell populations (such as liver or spleen)."}
{"id": "PMID:1080060", "title": "Transfer factor I: methods of therapy.", "content": "Transfer factor was first discovered by Lawrence in 1955, but was not used therapeutically until 1969 when we reported its use in a Wiskott-Aldrich patient. Since that time, it has been used in a wide variety of disorders related to defects in cellular immunity, infectious diseases, and malignant diseases. This report describes our experience with transfer factor. Report number I discusses rationale for patient selection, procedures for transfer factor therapy, procedures for monitoring the efficacy of therapy, untoward effects of therapy, and experience with transfer factor therapy in severe combined dual system deficiency disorder. The results of our study on transfer factor therapy indicate that it is capable of inducing a clinically acceptable level of cell-mediated immunity in approximately 50% of patients with a variety of immunodeficiency disorders. It also appears to be a useful adjunct to chemotherapy, and may possibly act synergistically with transplanted fetal thymocytes to produce a constantly regenerating specifically competent source of T lymphocytes, thereby obviating the need for bone marrow transplant for severe combined dual system deficiency disorder.", "contents": "Transfer factor I: methods of therapy. Transfer factor was first discovered by Lawrence in 1955, but was not used therapeutically until 1969 when we reported its use in a Wiskott-Aldrich patient. Since that time, it has been used in a wide variety of disorders related to defects in cellular immunity, infectious diseases, and malignant diseases. This report describes our experience with transfer factor. Report number I discusses rationale for patient selection, procedures for transfer factor therapy, procedures for monitoring the efficacy of therapy, untoward effects of therapy, and experience with transfer factor therapy in severe combined dual system deficiency disorder. The results of our study on transfer factor therapy indicate that it is capable of inducing a clinically acceptable level of cell-mediated immunity in approximately 50% of patients with a variety of immunodeficiency disorders. It also appears to be a useful adjunct to chemotherapy, and may possibly act synergistically with transplanted fetal thymocytes to produce a constantly regenerating specifically competent source of T lymphocytes, thereby obviating the need for bone marrow transplant for severe combined dual system deficiency disorder."}
{"id": "PMID:1080062", "title": "Studies of the human factor VIII/von Willebrand's factor protein. II. Identification and characterization of the von Willebrand protein.", "content": "The purified factor VIII-related protein we have previously characterized from normal cryoprecipitate possesses both procoagulant activity and vWf activity. We have attempted to isolate and characterize this protein from three patients with severe vWd. This protein is absent or markedly diminished in amount in these vWd patients, as judged by gel filtration, polyacrylamide-gel electrophoresis, and immunoprecipitation assays. Likewise, the procoagulant and vWf activities are deficient. As vWf activity is one of the major biologic functions of either the normal or hemophilic factor VIII-related protein, the purified protein should be designated the f VIII/vWf protein.", "contents": "Studies of the human factor VIII/von Willebrand's factor protein. II. Identification and characterization of the von Willebrand protein. The purified factor VIII-related protein we have previously characterized from normal cryoprecipitate possesses both procoagulant activity and vWf activity. We have attempted to isolate and characterize this protein from three patients with severe vWd. This protein is absent or markedly diminished in amount in these vWd patients, as judged by gel filtration, polyacrylamide-gel electrophoresis, and immunoprecipitation assays. Likewise, the procoagulant and vWf activities are deficient. As vWf activity is one of the major biologic functions of either the normal or hemophilic factor VIII-related protein, the purified protein should be designated the f VIII/vWf protein."}
{"id": "PMID:1080063", "title": "Specificity of bovine thrombin and reptilase for mammalian plasmas.", "content": "The effect of bovine thrombin and reptilase was investigated on plasmas of ten species of five vertebrate classes. It was established that both enzymes have an expressed specificity for mammalian plasmas, however, their narrower species specificity is different. These comparative coagulation studies, their significance in the laboratory examination also contribute to the analysis of evolution of clotting factors.", "contents": "Specificity of bovine thrombin and reptilase for mammalian plasmas. The effect of bovine thrombin and reptilase was investigated on plasmas of ten species of five vertebrate classes. It was established that both enzymes have an expressed specificity for mammalian plasmas, however, their narrower species specificity is different. These comparative coagulation studies, their significance in the laboratory examination also contribute to the analysis of evolution of clotting factors."}
{"id": "PMID:1080064", "title": "Kinetics of agonist conductance changes during hyperolarization at frog endplates.", "content": "The effect of rapid hyperpolarization on acetylcholine- or carbachol-induced currents was studied at voltage clamped frog endplates. Following a hyperpolarizing step the agonist-induced conductance increased approximately exponentially to a new level. The rate constant for this process was smaller during hyperpolarization or lowered temperature, and was also smaller for acetylcholine than for carbachol. The results are interpreted in terms of a potential-dependent conformational change of the receptor.", "contents": "Kinetics of agonist conductance changes during hyperolarization at frog endplates. The effect of rapid hyperpolarization on acetylcholine- or carbachol-induced currents was studied at voltage clamped frog endplates. Following a hyperpolarizing step the agonist-induced conductance increased approximately exponentially to a new level. The rate constant for this process was smaller during hyperpolarization or lowered temperature, and was also smaller for acetylcholine than for carbachol. The results are interpreted in terms of a potential-dependent conformational change of the receptor."}
{"id": "PMID:1080065", "title": "A prospective study of acute pancreatitis.", "content": "All 78 patients with acute pancreatitis admitted to one hospital in a 2-year period were included in a prospective survey of conservative management. The overall mortality rate was 11.5 per cent, being 46 per cent for 11 patients subjected to early laparotomy and only 6 per cent for patients managed without operation. Twenty-six patients were considered to have severe acute pancreatitis and all 9 deaths occurred in this group. Biliary disease was present in 51 per cent of patients, and the majority of those with biliary disease were over 60 years old. Alcohol-related acute pancreatitis occurred in 26 per cent of the total patients, and none of this group was over 60 years old. Acute pancreatitis was considered idiopathic in origin in only 13 per cent of the patients. Elective biliary surgery in 30 patients resulted in freedom from recurrent attacks of acute pancreatitis for the follow-up period (12-30 months). The results compare favourably with those in which glucagon and high dosage aprotinin (Trasylol) have been utilized in the management of acute pancreatitis.", "contents": "A prospective study of acute pancreatitis. All 78 patients with acute pancreatitis admitted to one hospital in a 2-year period were included in a prospective survey of conservative management. The overall mortality rate was 11.5 per cent, being 46 per cent for 11 patients subjected to early laparotomy and only 6 per cent for patients managed without operation. Twenty-six patients were considered to have severe acute pancreatitis and all 9 deaths occurred in this group. Biliary disease was present in 51 per cent of patients, and the majority of those with biliary disease were over 60 years old. Alcohol-related acute pancreatitis occurred in 26 per cent of the total patients, and none of this group was over 60 years old. Acute pancreatitis was considered idiopathic in origin in only 13 per cent of the patients. Elective biliary surgery in 30 patients resulted in freedom from recurrent attacks of acute pancreatitis for the follow-up period (12-30 months). The results compare favourably with those in which glucagon and high dosage aprotinin (Trasylol) have been utilized in the management of acute pancreatitis."}
{"id": "PMID:1080067", "title": "Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis.", "content": "A convenient technique suitable for the routine estimation of IgM and IgG antiglobulins has been devised. The assay involves the binding of antiglobulins (rheumatoid factors) to rabbit immunoglobulin linked to the surface of plastic tubes; the amount of antiglobulin bound is then determined by adding radiolabelled antihuman IgG or IgM. Both antiglobulins were raised in virtually all seropositive rheumatoid arthritics, and 19 out of 22 seronegative patients had raised values for either IgM or IgG rheumatoid factors. The test should prove valuable in diagnosis and the results further emphasize autosensitization to IgG as a dominant immunological characteristic of different forms of rheumatoid arthritis.", "contents": "Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. A convenient technique suitable for the routine estimation of IgM and IgG antiglobulins has been devised. The assay involves the binding of antiglobulins (rheumatoid factors) to rabbit immunoglobulin linked to the surface of plastic tubes; the amount of antiglobulin bound is then determined by adding radiolabelled antihuman IgG or IgM. Both antiglobulins were raised in virtually all seropositive rheumatoid arthritics, and 19 out of 22 seronegative patients had raised values for either IgM or IgG rheumatoid factors. The test should prove valuable in diagnosis and the results further emphasize autosensitization to IgG as a dominant immunological characteristic of different forms of rheumatoid arthritis."}
{"id": "PMID:1080070", "title": "Uveitis: report of a 10-year survey in Northern Finland.", "content": "In order to determine the distribution of etiologic and diagnostic categories of uveitis at the Oulu University Eye Hospital, 653 patients with uveitis treated during the years 1964-1974 were reviewed. Of the 653 patients, 321 were men and 332 women; 547 had anterior, 64 posterior and 42 generalzied uveitis; 524 unilateral and 129 bilateral; 570 acute and 83 chronic; and 446 single and 207 recurrent. The etiologic factors were distributed into rheumatoid (7.2%), streptococcal (4.1%), tuberculosis (3.7%), toxoplasmosis (3.3%), varicella-zoster (1.7%), sarcoidosis (1.4%), staphylococcal (1.1%), leptospirosis (0.6%), lymphatic leucemia (0.5%), herpes simplex (0.5%), yersinosis (0.1%) and undetermined cases (75.8%). There were no cases of syphilis. Most of the cases in this series were anterior and acute uveitis occurring mainly in the age group 20-59 years.", "contents": "Uveitis: report of a 10-year survey in Northern Finland. In order to determine the distribution of etiologic and diagnostic categories of uveitis at the Oulu University Eye Hospital, 653 patients with uveitis treated during the years 1964-1974 were reviewed. Of the 653 patients, 321 were men and 332 women; 547 had anterior, 64 posterior and 42 generalzied uveitis; 524 unilateral and 129 bilateral; 570 acute and 83 chronic; and 446 single and 207 recurrent. The etiologic factors were distributed into rheumatoid (7.2%), streptococcal (4.1%), tuberculosis (3.7%), toxoplasmosis (3.3%), varicella-zoster (1.7%), sarcoidosis (1.4%), staphylococcal (1.1%), leptospirosis (0.6%), lymphatic leucemia (0.5%), herpes simplex (0.5%), yersinosis (0.1%) and undetermined cases (75.8%). There were no cases of syphilis. Most of the cases in this series were anterior and acute uveitis occurring mainly in the age group 20-59 years."}
{"id": "PMID:1080072", "title": "Ventricular obstruction due to ectasia of the internal carotid artery.", "content": "This is a report of the first recorded instance of ventricular obstruction at the foramen of Monro by an ectatic carotid artery. The clinical course, investigations and treatment are described.", "contents": "Ventricular obstruction due to ectasia of the internal carotid artery. This is a report of the first recorded instance of ventricular obstruction at the foramen of Monro by an ectatic carotid artery. The clinical course, investigations and treatment are described."}
{"id": "PMID:1080073", "title": "Optimal conditions for the growth of malignant human and animal cell populations in immunosuppressed mice.", "content": "Immunosuppressed mice have been used to support the growth of xenogeneic human and animal malignant cell populations. The optimal conditions for tumor growth are neonatal thymectomy coupled with antithymocyte serum or thymectomy, followed by whole-body irradiation and bone marrow reconstitution. When mice are inoculated with a mixture of normal and malignant cells, the malignant cells have a selective advantage. No such selectivity is found when the mixed populations are grown in vitro. Human tumors may also be grown in immunosuppressed mice. These tumors retain the organization of the original tumor in the human host. The advantages of this system to cancer researchers are discussed.", "contents": "Optimal conditions for the growth of malignant human and animal cell populations in immunosuppressed mice. Immunosuppressed mice have been used to support the growth of xenogeneic human and animal malignant cell populations. The optimal conditions for tumor growth are neonatal thymectomy coupled with antithymocyte serum or thymectomy, followed by whole-body irradiation and bone marrow reconstitution. When mice are inoculated with a mixture of normal and malignant cells, the malignant cells have a selective advantage. No such selectivity is found when the mixed populations are grown in vitro. Human tumors may also be grown in immunosuppressed mice. These tumors retain the organization of the original tumor in the human host. The advantages of this system to cancer researchers are discussed."}
{"id": "PMID:1080075", "title": "Results of graft surgery for coronary artery disease: a progress report.", "content": "Coronary artery surgery is here to stay, but it will continue to be influenced by accumulating experience and evolving techniques. Direct internal mammary-to-coronary artery anastomoses offer the hope for improved results in certain patients. Tissue-compatible prosthetic grafts could be developed and offer an alternative in selected patients. Improved microsurgical techniques may permit broader applications with multiple grafts to smaller branches in patients with more diffuse disease. We are continually reminded, however, that surgical treatment is palliative, and that most of these patients will ultimately die of their disease. Thus the search for a better understanding of the arteriosclerotic process and improved methods for altering the course of the disease becomes more important than ever.", "contents": "Results of graft surgery for coronary artery disease: a progress report. Coronary artery surgery is here to stay, but it will continue to be influenced by accumulating experience and evolving techniques. Direct internal mammary-to-coronary artery anastomoses offer the hope for improved results in certain patients. Tissue-compatible prosthetic grafts could be developed and offer an alternative in selected patients. Improved microsurgical techniques may permit broader applications with multiple grafts to smaller branches in patients with more diffuse disease. We are continually reminded, however, that surgical treatment is palliative, and that most of these patients will ultimately die of their disease. Thus the search for a better understanding of the arteriosclerotic process and improved methods for altering the course of the disease becomes more important than ever."}
{"id": "PMID:1080076", "title": "The fine structure of the iris of the grass frog, Rana pipiens.", "content": "The iris of the grass frog Rana iiens, can respond to light even when isolated from the remainder of the animal. The iris is a three-layered structure, comprising a stromal layer and two layers of pigment epithelium. The sphincter pupillae, which is composed of pigmented smooth muscle cells, is embedded between the two layers of pigment epithelium. There is no dilator pupillae in this species. We have been unable to find any cells or any organelles in the iris which are anatomically specialized for photoreception in any obvious way.", "contents": "The fine structure of the iris of the grass frog, Rana pipiens. The iris of the grass frog Rana iiens, can respond to light even when isolated from the remainder of the animal. The iris is a three-layered structure, comprising a stromal layer and two layers of pigment epithelium. The sphincter pupillae, which is composed of pigmented smooth muscle cells, is embedded between the two layers of pigment epithelium. There is no dilator pupillae in this species. We have been unable to find any cells or any organelles in the iris which are anatomically specialized for photoreception in any obvious way."}
{"id": "PMID:1080077", "title": "A possible functional relationship between the subcommissural organ and the pineal complex and lateral eyes in Rana esculenta and Rana temporaria.", "content": "In previous publications (Diederen, 1972, 1973) evidence was presented that light and darkness hardly influence the secretory activity of the subcommissural organ (SCO) in intact frogs, i.e. frogs in which all photo-receptive organs are present. In the present study the influence of long daily photoperiods and of continuous darkness on the SCO of frogs in which the frontal organ, the pineal organ and the eyes were eliminated separately or in combination was studied. The parameters used to measure the secretory activity of the SCO were the amount of aldehyde-fuchsin(AF)-positive material in the SCO cells, the incorporation of 35S-cysteine by the SCO cells and the growth rate of Reissner's fibre (RF). Only in frogs in which the eyes as well as the entire pineal complex were eliminated was an unequivocal influence of light and darkness on the secretory activity of the SCO demonstrable: in blinded frogs without a pineal complex, the growth rate of RF is clearly enhanced in animals exposed to light. If the eyes or the pineal complex or the pineal organ alone have not been eliminated, then the stimulatory influence of light on the growth rate of RF can no longer be detected. This indicates that light causes the pineal complex and the eyes to exert an inhibitory influence on the secretory activity of the SCO. The hypothesis is put forward that in intact frogs influences of light on the secretory activity of the SCO, at least so far as the production of RF is concerned, are prevented by the photo-receptive organs. In this way a functional relationship between the SCO on the one hand and the pineal complex and eyes on the other hand may be expressed. A mechanism by which the influences of light on the SCO may be prevented by the pineal complex and the eyes is suggested.", "contents": "A possible functional relationship between the subcommissural organ and the pineal complex and lateral eyes in Rana esculenta and Rana temporaria. In previous publications (Diederen, 1972, 1973) evidence was presented that light and darkness hardly influence the secretory activity of the subcommissural organ (SCO) in intact frogs, i.e. frogs in which all photo-receptive organs are present. In the present study the influence of long daily photoperiods and of continuous darkness on the SCO of frogs in which the frontal organ, the pineal organ and the eyes were eliminated separately or in combination was studied. The parameters used to measure the secretory activity of the SCO were the amount of aldehyde-fuchsin(AF)-positive material in the SCO cells, the incorporation of 35S-cysteine by the SCO cells and the growth rate of Reissner's fibre (RF). Only in frogs in which the eyes as well as the entire pineal complex were eliminated was an unequivocal influence of light and darkness on the secretory activity of the SCO demonstrable: in blinded frogs without a pineal complex, the growth rate of RF is clearly enhanced in animals exposed to light. If the eyes or the pineal complex or the pineal organ alone have not been eliminated, then the stimulatory influence of light on the growth rate of RF can no longer be detected. This indicates that light causes the pineal complex and the eyes to exert an inhibitory influence on the secretory activity of the SCO. The hypothesis is put forward that in intact frogs influences of light on the secretory activity of the SCO, at least so far as the production of RF is concerned, are prevented by the photo-receptive organs. In this way a functional relationship between the SCO on the one hand and the pineal complex and eyes on the other hand may be expressed. A mechanism by which the influences of light on the SCO may be prevented by the pineal complex and the eyes is suggested."}
{"id": "PMID:1080078", "title": "Morphological definition of thymocyte subpopulations.", "content": "An attempt was made to distinguish immature from mature, immunocompetent thymocytes in the adult mouse using morphological criteria.  All procedures enriching for competent thymocytes also enrich small cells whose morphology is very similar to the small thymocytes that have been described in the medulla of the thymus (Abe and Ito, 1970).  A heterogeneity of competent thymocytes is likely since the enriched populations always contain a number of medium sized cells.  These latter cells show spontaneous DNA-synthetic activity and are probably the source of thymus proliferative activity.  Owing to their characteristic morphology these two cell types might represent the precursors for each of the two differentiated T cell types that have been found to occur in delayed type hypersensitivity (Matter, 1974).", "contents": "Morphological definition of thymocyte subpopulations. An attempt was made to distinguish immature from mature, immunocompetent thymocytes in the adult mouse using morphological criteria.  All procedures enriching for competent thymocytes also enrich small cells whose morphology is very similar to the small thymocytes that have been described in the medulla of the thymus (Abe and Ito, 1970).  A heterogeneity of competent thymocytes is likely since the enriched populations always contain a number of medium sized cells.  These latter cells show spontaneous DNA-synthetic activity and are probably the source of thymus proliferative activity.  Owing to their characteristic morphology these two cell types might represent the precursors for each of the two differentiated T cell types that have been found to occur in delayed type hypersensitivity (Matter, 1974)."}
{"id": "PMID:1080079", "title": "A monoamine in the gustatory cell of the frog's taste organ: a fluorescence histochemical and electron microscopic study.", "content": "Fluorescence histochemistry reveals that in the frog's taste organ a yellow fluorescence is regularly observed at the most basal region of the sensory epithelium. The fluorescence has a strong intensity, but it fades rapidly upon the UV-irradiation. The peak of the emission spectrum is at 520 mmug. Following reserpine treatment the yellow fluroescence is markedly reduced, but not depleted completely. From these characteristics the monoamine fluorescence is regarded as representing 5-HT (serotonin). The ultrastructural study on sensory epithelia shows that the terminal portions of gustatory cell processes are localized at the basal region. These portions are filled with dense cored vesicles (700-1000 angstrom in diameter) and frequently opposed with nerve fibers penetrating into the epithelium. The gustatory cell processes are also interposed between the terminal portions of nerve fibers. The cytoplasm of the gustatory cell process is characterized by many mitochondria, fine filaments and glycogen particles, but contains few cored vesicles. The distribution of terminal portions of gustary cell processes seems to correspond fairly well to that of the monoamine fluorescence observed discontinuously along the basal lamina. Accordingly it is concluded that the fluorigenic monoamine is localized in the cored vesicles of the gustatory cell.", "contents": "A monoamine in the gustatory cell of the frog's taste organ: a fluorescence histochemical and electron microscopic study. Fluorescence histochemistry reveals that in the frog's taste organ a yellow fluorescence is regularly observed at the most basal region of the sensory epithelium. The fluorescence has a strong intensity, but it fades rapidly upon the UV-irradiation. The peak of the emission spectrum is at 520 mmug. Following reserpine treatment the yellow fluroescence is markedly reduced, but not depleted completely. From these characteristics the monoamine fluorescence is regarded as representing 5-HT (serotonin). The ultrastructural study on sensory epithelia shows that the terminal portions of gustatory cell processes are localized at the basal region. These portions are filled with dense cored vesicles (700-1000 angstrom in diameter) and frequently opposed with nerve fibers penetrating into the epithelium. The gustatory cell processes are also interposed between the terminal portions of nerve fibers. The cytoplasm of the gustatory cell process is characterized by many mitochondria, fine filaments and glycogen particles, but contains few cored vesicles. The distribution of terminal portions of gustary cell processes seems to correspond fairly well to that of the monoamine fluorescence observed discontinuously along the basal lamina. Accordingly it is concluded that the fluorigenic monoamine is localized in the cored vesicles of the gustatory cell."}
{"id": "PMID:1080080", "title": "Scanning electron microscopy of the wall of the third ventricle of the brain of Rana temporia. II. Electron microscopy of the ventricular surface of the pars ventralis of the tuber cinereum.", "content": "Scanning electrom microscopy of the ventricular surface of the pars ventralis of the tuber cinereum of the frog Rana temporaria shows that it can be divided in (1) a dorsolateral area, characterized by the presence of very numerous large, solid, bulbous protrusions (2) a medial area, where the large bulbous structures are completely absent and which is diffusely covered with very numerous cilia, (3) a transiotional area located between the two other regions. The large bulbs, shown by scanning electron microscopy, correspond with the end-bulbs of thick ventricular dendrites of nerve cells revealed by transmission electron microscopy. At least many of these intra-ventricular bulbs are dendritic endings of peptidergic neurosecretory neurons, which have been tentatively identified as adenohypophysiotropic neuro-hormone producing neurons (Derickx et al., 1972, 1973a, 1973b). The structural features of the dendritic endings plead in favour of a possible receptive role.", "contents": "Scanning electron microscopy of the wall of the third ventricle of the brain of Rana temporia. II. Electron microscopy of the ventricular surface of the pars ventralis of the tuber cinereum. Scanning electrom microscopy of the ventricular surface of the pars ventralis of the tuber cinereum of the frog Rana temporaria shows that it can be divided in (1) a dorsolateral area, characterized by the presence of very numerous large, solid, bulbous protrusions (2) a medial area, where the large bulbous structures are completely absent and which is diffusely covered with very numerous cilia, (3) a transiotional area located between the two other regions. The large bulbs, shown by scanning electron microscopy, correspond with the end-bulbs of thick ventricular dendrites of nerve cells revealed by transmission electron microscopy. At least many of these intra-ventricular bulbs are dendritic endings of peptidergic neurosecretory neurons, which have been tentatively identified as adenohypophysiotropic neuro-hormone producing neurons (Derickx et al., 1972, 1973a, 1973b). The structural features of the dendritic endings plead in favour of a possible receptive role."}
{"id": "PMID:1080081", "title": "Electron microscopic studies of the lung of the frog. II. Topography of the inner surface by scanning and transmission electron microscopy.", "content": "Scanning and transmission electron microscopy were used to study the inner architecture of the frog lung. In some specimens the alveolar surface mucus layer was removed to permit the examination of underlying features. The inner surface of the frog's lung is covered by a layer of microvilli belonging to only one type of epithelial cells. The boundaries of these epithelial cells are demarcated by small ridges. Different degrees of lung expansion cause variations of the surface topography. The morphology of certain surface features is examined in detail. Several methods of drying the specimens are compared.", "contents": "Electron microscopic studies of the lung of the frog. II. Topography of the inner surface by scanning and transmission electron microscopy. Scanning and transmission electron microscopy were used to study the inner architecture of the frog lung. In some specimens the alveolar surface mucus layer was removed to permit the examination of underlying features. The inner surface of the frog's lung is covered by a layer of microvilli belonging to only one type of epithelial cells. The boundaries of these epithelial cells are demarcated by small ridges. Different degrees of lung expansion cause variations of the surface topography. The morphology of certain surface features is examined in detail. Several methods of drying the specimens are compared."}
{"id": "PMID:1080082", "title": "Ultrastructure of the neurohemal hypothalamic floor of the frog, Rana catesbeiana.", "content": "The fine structure of the hypothalamic floor was studied in the frog, Rana catesbeiana. The regions slightly anterior and posterior to thw swollen hypothalamic floor part, which has been classically designated as the median eminence, contained neurosecretory axon terminals abutting on the capillary walls. The region relatively far anterior to the swollen part did not show neurosecretory axons terminating on capillary walls. The neural stalk contiguous to the rostral border of the pars intermedia had few neurosecretory axon terminals which end on the terminal portions of the ependymal processes covering the capillary wall. The functional significance of the regional differentiation of the frog neurohypophysis is discussed in connection with the regional differentiation of various secretory cells in the adenohypophysis.", "contents": "Ultrastructure of the neurohemal hypothalamic floor of the frog, Rana catesbeiana. The fine structure of the hypothalamic floor was studied in the frog, Rana catesbeiana. The regions slightly anterior and posterior to thw swollen hypothalamic floor part, which has been classically designated as the median eminence, contained neurosecretory axon terminals abutting on the capillary walls. The region relatively far anterior to the swollen part did not show neurosecretory axons terminating on capillary walls. The neural stalk contiguous to the rostral border of the pars intermedia had few neurosecretory axon terminals which end on the terminal portions of the ependymal processes covering the capillary wall. The functional significance of the regional differentiation of the frog neurohypophysis is discussed in connection with the regional differentiation of various secretory cells in the adenohypophysis."}
{"id": "PMID:1080084", "title": "Abnormal left ventricular wall motion at mid-ejection in patients with coronary heart disease.", "content": "The degree of inward motion at mid-ejection was examined for seven segments on the silhouettes of left ventriculograms taken in the 30 degrees RAO projection in patients with normal coronary arteries. The pattern of wall motion described in these patients was used to distinguish abnormalities in mid-systolic wall motion. One or more abnormally contracting segments were found at mid-ejection of 27 of 42 patients with obstructive coronary artery disease and normal end-systolic wall motion. Of the 57 segments found in these patients, 41 or 72% corresponded to sites of significant coronary artery obstruction. Seven patients had electrocardiographic evidence of prior infarction. Following coronary graft surgery in eight patients improved motion was found in association with graft patency in seven priviously delayed segments and two new areas of delayed wall motion associated with nonpatent grafts and electrocardiographic changes of infarction appeared. We postulate that some of the myocardial fibers in late contracting segments have been injured or infarcted and are able to contract effectively only during the latter half of ejection when ventricular wall tension is reduced.", "contents": "Abnormal left ventricular wall motion at mid-ejection in patients with coronary heart disease. The degree of inward motion at mid-ejection was examined for seven segments on the silhouettes of left ventriculograms taken in the 30 degrees RAO projection in patients with normal coronary arteries. The pattern of wall motion described in these patients was used to distinguish abnormalities in mid-systolic wall motion. One or more abnormally contracting segments were found at mid-ejection of 27 of 42 patients with obstructive coronary artery disease and normal end-systolic wall motion. Of the 57 segments found in these patients, 41 or 72% corresponded to sites of significant coronary artery obstruction. Seven patients had electrocardiographic evidence of prior infarction. Following coronary graft surgery in eight patients improved motion was found in association with graft patency in seven priviously delayed segments and two new areas of delayed wall motion associated with nonpatent grafts and electrocardiographic changes of infarction appeared. We postulate that some of the myocardial fibers in late contracting segments have been injured or infarcted and are able to contract effectively only during the latter half of ejection when ventricular wall tension is reduced."}
{"id": "PMID:1080085", "title": "Main left coronary artery disease. Clinical experience from 1964-1974.", "content": "Obstructive lesions of the main left coronary artery (LCA) were demonstrated angiographically in 73 patients, comprising 4.3% of the total population referred to us for diagnostic evaluation of chest pain. Although there were no specific historical or clinical features which could absolutely distinguish this subgroup from the larger population of coronary artery disease patients, 81% (34/42) of the double Master's exercise tests, in which the patient achieved a heart rate of at least 110 beats/min. demonstrated greater than or equal 2 mm R-ST segmental depression. Of the total group of 73, 32 were evaluated during the six-year period from 1964 to 1971 and a preliminary report made in 1972. The diagnosis in the remaining 41 patients was established in the 1/2 year period from 1971 to 1973. The initial 32 patients were seen before the significance of a main LCA lesion was appreciated. In this subgroup there were five deaths at the time of cardiac catheterization. However, in the more recent group improved recognition of patients with possible main LCA disease prior to catheterization has led to a much lower death rate related to diagnostic catheterization. Only one of the last 41 patients undergoing coronary angiography has died. Nineteen patients were managed medically. Of this group 17 were considered to be under the same risk of death as the surgical candidates. Their mortality rate, as high. The risk of hying was 43.6% after 24 months, 51.1% after 36 months, and 73.6% after 42 months of observation. Although the initial surgical experience, using internal mammary artery implants and saphenous venin bypass grafts, was associated with a high mortality, direct revascularization surgery over the last 2 1/2 years has been accomplished with a perioperative mortality of only 6.2%. All deaths in the group of 40 patients receiving elective revasularization with saphenous vein bypass grafts occurred in the first six months following surgery. The risk of dying was 12.5% after six months of observation and was unchanged for the remainder of the follow-up period. After 21 months the difference in survival between the two groups is statistically significant (P less than 0.05). The one late death among the surgical survivors occurred four months after the operative procedure and was related to noncardiovaxcular surgical complications. The surgical survivors have been followed for an average of 27 months. Thus revascularization surgery has improved the prognosis for patients with main LCA disease. We currently advise prompt evaluation for any patient suspected of having this type of obstruction and urgent idrect revascularization surgery if this lesion is demonstrated angiographically.", "contents": "Main left coronary artery disease. Clinical experience from 1964-1974. Obstructive lesions of the main left coronary artery (LCA) were demonstrated angiographically in 73 patients, comprising 4.3% of the total population referred to us for diagnostic evaluation of chest pain. Although there were no specific historical or clinical features which could absolutely distinguish this subgroup from the larger population of coronary artery disease patients, 81% (34/42) of the double Master's exercise tests, in which the patient achieved a heart rate of at least 110 beats/min. demonstrated greater than or equal 2 mm R-ST segmental depression. Of the total group of 73, 32 were evaluated during the six-year period from 1964 to 1971 and a preliminary report made in 1972. The diagnosis in the remaining 41 patients was established in the 1/2 year period from 1971 to 1973. The initial 32 patients were seen before the significance of a main LCA lesion was appreciated. In this subgroup there were five deaths at the time of cardiac catheterization. However, in the more recent group improved recognition of patients with possible main LCA disease prior to catheterization has led to a much lower death rate related to diagnostic catheterization. Only one of the last 41 patients undergoing coronary angiography has died. Nineteen patients were managed medically. Of this group 17 were considered to be under the same risk of death as the surgical candidates. Their mortality rate, as high. The risk of hying was 43.6% after 24 months, 51.1% after 36 months, and 73.6% after 42 months of observation. Although the initial surgical experience, using internal mammary artery implants and saphenous venin bypass grafts, was associated with a high mortality, direct revascularization surgery over the last 2 1/2 years has been accomplished with a perioperative mortality of only 6.2%. All deaths in the group of 40 patients receiving elective revasularization with saphenous vein bypass grafts occurred in the first six months following surgery. The risk of dying was 12.5% after six months of observation and was unchanged for the remainder of the follow-up period. After 21 months the difference in survival between the two groups is statistically significant (P less than 0.05). The one late death among the surgical survivors occurred four months after the operative procedure and was related to noncardiovaxcular surgical complications. The surgical survivors have been followed for an average of 27 months. Thus revascularization surgery has improved the prognosis for patients with main LCA disease. We currently advise prompt evaluation for any patient suspected of having this type of obstruction and urgent idrect revascularization surgery if this lesion is demonstrated angiographically."}
{"id": "PMID:1080086", "title": "Inhibition of kinin formation by a kallikrein inhibitor during extracorporeal circulation in open-heart surgery.", "content": "Involvement of the kinin system was studied in 58 patients during extracorporeal circulation in open heart surgery. At the onset of extracorporeal circulation, there was no increase of free kinin in blood and no reduction of plasma kininogen, when the latter was expressed as mug bradykinin/mg plasma protein. With the lapse of time, kinin in the blood increased significantly, and decrease of kininogen was also significant. The longer the circulation time, the greater the consumption of kininogen. The site of the kinin formation was presumed to be the heart-lung machine since the level of kinin increased slightly and kininogen decreased gradually in the blood leaving the machine, compared with those levels entering the machine. The difference in kininogen was significantly different from zero at termination of extracorporeal circulation. Administration of a kallikrein-trypsin inhibitor, trasylol (A. G. Bayer), infused into the heart-lung machine, prevented the decrease of kininogen. Reduction of the total peripheral resistance during this circulation was also prevented. The hemoconcentration, presumably the result of vascular permeability increase caused by increased kinin, was prevented by trasylol in cases in which extracorporeal circulation lasted over 60 minutes. Trasylol applied thus appears to be an effective counteragent for circulatory disturbances which occur during extracorporeal circulation.", "contents": "Inhibition of kinin formation by a kallikrein inhibitor during extracorporeal circulation in open-heart surgery. Involvement of the kinin system was studied in 58 patients during extracorporeal circulation in open heart surgery. At the onset of extracorporeal circulation, there was no increase of free kinin in blood and no reduction of plasma kininogen, when the latter was expressed as mug bradykinin/mg plasma protein. With the lapse of time, kinin in the blood increased significantly, and decrease of kininogen was also significant. The longer the circulation time, the greater the consumption of kininogen. The site of the kinin formation was presumed to be the heart-lung machine since the level of kinin increased slightly and kininogen decreased gradually in the blood leaving the machine, compared with those levels entering the machine. The difference in kininogen was significantly different from zero at termination of extracorporeal circulation. Administration of a kallikrein-trypsin inhibitor, trasylol (A. G. Bayer), infused into the heart-lung machine, prevented the decrease of kininogen. Reduction of the total peripheral resistance during this circulation was also prevented. The hemoconcentration, presumably the result of vascular permeability increase caused by increased kinin, was prevented by trasylol in cases in which extracorporeal circulation lasted over 60 minutes. Trasylol applied thus appears to be an effective counteragent for circulatory disturbances which occur during extracorporeal circulation."}
{"id": "PMID:1080087", "title": "On the inhibition of elastase by serum. Some distinguishing properties of alpha1-antitrypsin and alpha2-macroglobulin.", "content": "1. The influence of serum on the elastolytic and esterolytic activity of elastase has been studied. With both substrates the inhibition curves are linear. 1 ml of normal human serum inhibits the activity of 0.77 mg of pure porcine elastase. 2. Elastase binds faster with alpha2-macroglobulin (k = 3.4-10(6) M-1 S-1) than it does with alpha1-antitrypsin (k = 5-10(5) M-1 S-1). 3. The dissociation constant of the alpha-antitrypsin -elastase complex is much lower than that of the alpha2-macroglobulin-elastase complex but both complexes are very stable (Ki less than 10(-10) M). 4. Protein pi (inter-alpha-inhibitor) does not inhibit elastase.", "contents": "On the inhibition of elastase by serum. Some distinguishing properties of alpha1-antitrypsin and alpha2-macroglobulin. 1. The influence of serum on the elastolytic and esterolytic activity of elastase has been studied. With both substrates the inhibition curves are linear. 1 ml of normal human serum inhibits the activity of 0.77 mg of pure porcine elastase. 2. Elastase binds faster with alpha2-macroglobulin (k = 3.4-10(6) M-1 S-1) than it does with alpha1-antitrypsin (k = 5-10(5) M-1 S-1). 3. The dissociation constant of the alpha-antitrypsin -elastase complex is much lower than that of the alpha2-macroglobulin-elastase complex but both complexes are very stable (Ki less than 10(-10) M). 4. Protein pi (inter-alpha-inhibitor) does not inhibit elastase."}
{"id": "PMID:1080090", "title": "Fibre-optic endoscopy and radiology in the investigation of the ipper gastrointestinatract.", "content": "A comparative study is reported of endoscopy and the barium meal in the investigation of the oesophagus, stomach and duodenum, based on 281 endoscopies. There was total agreement between the two methods in 239 instances, including 101 normals, 80 peptic ulcers and nine cancers. In nine instances with unequivocal follow-up information there was definite disagreement, radiology being wrong in two (incorrect confident diagnoses of carcinoma) and endoscopy being wrong in seven (three carcinomas and four ulcers not diagnosed). Of 116 normal barium meals, 15 definite abnormalities were demonstrated by endoscopy, including six peptic ulcers. 'Probable' radiological diagnoses were confirmed by endoscopy in 44 instances. In those where endoscopy disagreed, radiology was definitely shown to be wrong in five, and endoscopy in five. A high degree of accuracy can be achieved by the selected use of both techniques, the information obtained from each considered of equal importance, and neither being regarded as the final arbiter.", "contents": "Fibre-optic endoscopy and radiology in the investigation of the ipper gastrointestinatract. A comparative study is reported of endoscopy and the barium meal in the investigation of the oesophagus, stomach and duodenum, based on 281 endoscopies. There was total agreement between the two methods in 239 instances, including 101 normals, 80 peptic ulcers and nine cancers. In nine instances with unequivocal follow-up information there was definite disagreement, radiology being wrong in two (incorrect confident diagnoses of carcinoma) and endoscopy being wrong in seven (three carcinomas and four ulcers not diagnosed). Of 116 normal barium meals, 15 definite abnormalities were demonstrated by endoscopy, including six peptic ulcers. 'Probable' radiological diagnoses were confirmed by endoscopy in 44 instances. In those where endoscopy disagreed, radiology was definitely shown to be wrong in five, and endoscopy in five. A high degree of accuracy can be achieved by the selected use of both techniques, the information obtained from each considered of equal importance, and neither being regarded as the final arbiter."}
{"id": "PMID:1080088", "title": "Familial Kallmann syndrome with unilateral renal aplasia.", "content": "On the basis of studies in two brothers and their double first cousin, the Kallmann syndrome (KS) is discussed as an X-linked syndrome of anosmic hypogonadotropic hypogonadism. The anosmia is thought to represent agenesis or hypoplasia of the olfactory lobes, the mildest form of the alobar holoprosencephaly developmental field defect; this is supported by the finding of hypotelorism in two of the patients and their mother. The endocrine defect is thought to represent a hypothalamic abnormality of the luteinizing hormone releasing hormone; borderline normal intelligence may represent another pleio-tropic CNS manifestation of the KS gene. All three affected males had unilateral renal aplasia, associated in one with ipsilateral absence of the testis. The presence of at least two developmental field defects (involving the CNS and urogenital system) makes it likely that the KS is a true multiple congenital anomaly syndrome; this is supported by the finding of additional, mostly minor, anomalies reported by other investigators. Heterozygous females may also show manifestations of anosmia, hypogonadism, possibly even internal genital malformation; however, genetic heterogeneity of anosmic hypogonadism is possible, and for the time being it is probably better to designate sporadic female cases of anosmic hypogonadism as examples of the olfacto-genital syndrome of DeMorsier. Linkage studies are urgently needed to clear up the question of genetic heterogeneity and to help develop empiric recurrence risk figures in anosmic hypogonadism.", "contents": "Familial Kallmann syndrome with unilateral renal aplasia. On the basis of studies in two brothers and their double first cousin, the Kallmann syndrome (KS) is discussed as an X-linked syndrome of anosmic hypogonadotropic hypogonadism. The anosmia is thought to represent agenesis or hypoplasia of the olfactory lobes, the mildest form of the alobar holoprosencephaly developmental field defect; this is supported by the finding of hypotelorism in two of the patients and their mother. The endocrine defect is thought to represent a hypothalamic abnormality of the luteinizing hormone releasing hormone; borderline normal intelligence may represent another pleio-tropic CNS manifestation of the KS gene. All three affected males had unilateral renal aplasia, associated in one with ipsilateral absence of the testis. The presence of at least two developmental field defects (involving the CNS and urogenital system) makes it likely that the KS is a true multiple congenital anomaly syndrome; this is supported by the finding of additional, mostly minor, anomalies reported by other investigators. Heterozygous females may also show manifestations of anosmia, hypogonadism, possibly even internal genital malformation; however, genetic heterogeneity of anosmic hypogonadism is possible, and for the time being it is probably better to designate sporadic female cases of anosmic hypogonadism as examples of the olfacto-genital syndrome of DeMorsier. Linkage studies are urgently needed to clear up the question of genetic heterogeneity and to help develop empiric recurrence risk figures in anosmic hypogonadism."}
{"id": "PMID:1080091", "title": "Adrenal weight and catecholamine content in renal and calciferol-induced hypertension in rabbits.", "content": "1. Hypertension was produced in rabbits by unilateral renal encapsulation combined with contralateral nephrectomy or by the administration of calciferol and calcium lactate. 2. Weighed post mortem, the adrenal glands from the two hypertensive groups were significantly heavier than those from normotensive animals of similar weight. There was a direct correlation between the adrenal gland weight and the mean arterial blood pressure measured at the time of the terminal experiment. 3. The catecholamine content of the adrenal glands from the two groups of hypertensive animals was similar to that of the normotensive animals. 4. There was histological evidence of adrenal cortical hypertrophy in the glands of the hypertensive groups of animals.", "contents": "Adrenal weight and catecholamine content in renal and calciferol-induced hypertension in rabbits. 1. Hypertension was produced in rabbits by unilateral renal encapsulation combined with contralateral nephrectomy or by the administration of calciferol and calcium lactate. 2. Weighed post mortem, the adrenal glands from the two hypertensive groups were significantly heavier than those from normotensive animals of similar weight. There was a direct correlation between the adrenal gland weight and the mean arterial blood pressure measured at the time of the terminal experiment. 3. The catecholamine content of the adrenal glands from the two groups of hypertensive animals was similar to that of the normotensive animals. 4. There was histological evidence of adrenal cortical hypertrophy in the glands of the hypertensive groups of animals."}
{"id": "PMID:1080092", "title": "The pace of patient processing in the mental health system.", "content": "In consultation to some mental health agencies, we have found the pace and quality of patient processing to be needlessly labored, discontinuous, and often redundant. Processing is too often \"for the record\" rather than \"for the client.\" Information rarely flows comprehensively, comprehensibly, or continuously between the evaluating system and the system from which the client is sent or to which he is referred. Contributing factors are identified and analyzed; the processing system is demystified. A change in strategy is suggested.", "contents": "The pace of patient processing in the mental health system. In consultation to some mental health agencies, we have found the pace and quality of patient processing to be needlessly labored, discontinuous, and often redundant. Processing is too often \"for the record\" rather than \"for the client.\" Information rarely flows comprehensively, comprehensibly, or continuously between the evaluating system and the system from which the client is sent or to which he is referred. Contributing factors are identified and analyzed; the processing system is demystified. A change in strategy is suggested."}
{"id": "PMID:1080093", "title": "Fixed dose prostaglandin E2 tablets in the induction of labour in multipara.", "content": "Prostaglandin E2 tablets in a dose of 0.5 mg. hourly were administered to 40 multiparous patients for induction of labour following amniotomy. Thirty-seven patients delivered vaginally within 18 hours, with a mean amniotomy delivery interval of 7 hours 53 minutes. The advantages of this method of administration are discussed and side-effects reported.", "contents": "Fixed dose prostaglandin E2 tablets in the induction of labour in multipara. Prostaglandin E2 tablets in a dose of 0.5 mg. hourly were administered to 40 multiparous patients for induction of labour following amniotomy. Thirty-seven patients delivered vaginally within 18 hours, with a mean amniotomy delivery interval of 7 hours 53 minutes. The advantages of this method of administration are discussed and side-effects reported."}
{"id": "PMID:1080095", "title": "Continuous negative chest-wall pressure as therapy for severe respiratory distress in older children.", "content": "Continuous negative chest-wall pressure (CNP) was used to treat five children, 4 to 11 years of age, who had progressively severe respiratory distress caused by Pneumocystis carinii penumonitis. After initial improvement, two patients developed progressive increases in respiratory rate, alveolar-arterial oxygen gradient, intrapulmonary right-to-left shunt, and hypoxia. The disease ended fatally in both. The remaining three patients continued to improve and recovered from their pulmonary disease. These results show that CNP therapy provides an effective means of improving arterial oxygenation in spontaneously breathing older children, just as in neonates, without the need for endotracheal intubation, prolonged sedation, and muscle relaxants.", "contents": "Continuous negative chest-wall pressure as therapy for severe respiratory distress in older children. Continuous negative chest-wall pressure (CNP) was used to treat five children, 4 to 11 years of age, who had progressively severe respiratory distress caused by Pneumocystis carinii penumonitis. After initial improvement, two patients developed progressive increases in respiratory rate, alveolar-arterial oxygen gradient, intrapulmonary right-to-left shunt, and hypoxia. The disease ended fatally in both. The remaining three patients continued to improve and recovered from their pulmonary disease. These results show that CNP therapy provides an effective means of improving arterial oxygenation in spontaneously breathing older children, just as in neonates, without the need for endotracheal intubation, prolonged sedation, and muscle relaxants."}
{"id": "PMID:1080096", "title": "Acetylation rates and monthly liver function tests during one year of isoniazid preventive therapy.", "content": "A blind, prospective evaluation of the incidence and course of isoniazid-associated liver injury was made in 358 hospitalized men. The men were psychiatric patients during one year of tuberculosis preventive therapy. Blood samples were obtained at monthly intervals from the patients, the majority of whom were taking isoniazid. When the data were analyzed at the end of the year, a strikingly increased incidence of abnormal serum transaminase (SGOT) and bilirubin values was found among the isoniazid recipients. However, most subjects demonstrating biochemical evidence of hepatic injury recovered completely while continuing to take isoniazid and did not progress to clinically overt hepatitis. The mechanism underlying this adaptation to isoniazid injury is unknown. No serum antibodies against isoniazid could be demonstrated, and no correlation was found between the presence of antinuclear antibodies or elevated isoniazid plasma concentrations and the occurrence of hepatic injury. These data support the view that hepatotoxic metabolities of isoniazid may be responsible for the liver injury.", "contents": "Acetylation rates and monthly liver function tests during one year of isoniazid preventive therapy. A blind, prospective evaluation of the incidence and course of isoniazid-associated liver injury was made in 358 hospitalized men. The men were psychiatric patients during one year of tuberculosis preventive therapy. Blood samples were obtained at monthly intervals from the patients, the majority of whom were taking isoniazid. When the data were analyzed at the end of the year, a strikingly increased incidence of abnormal serum transaminase (SGOT) and bilirubin values was found among the isoniazid recipients. However, most subjects demonstrating biochemical evidence of hepatic injury recovered completely while continuing to take isoniazid and did not progress to clinically overt hepatitis. The mechanism underlying this adaptation to isoniazid injury is unknown. No serum antibodies against isoniazid could be demonstrated, and no correlation was found between the presence of antinuclear antibodies or elevated isoniazid plasma concentrations and the occurrence of hepatic injury. These data support the view that hepatotoxic metabolities of isoniazid may be responsible for the liver injury."}
{"id": "PMID:1080099", "title": "Ionic aspects of excitation in rod outer segments.", "content": "The current status of the problem of ionic mechanisms underlying excitation of vertebrate photoreceptors is reviewed. Evidence is presented that the ionic dark current of retinal rods is sustained by the action of a ouabain-sensitive Na-K exchange pump driven by oxidative metabolism. The photoreceptors are depleted of K when the pump is stopped by ouabain. Considerations of cell membrane topology, kinetics of the light response, and signal amplification indicate that the light-induced suppression of the ionic dark current is mediated by a diffusible internal chemical transmitter substance. The desensitizing effect of low CA2+ Ringer's on the light responses of vertebrate rods, along with other indirect evidence, suggests that the transmitter substance may be calcium ions released from the internal membranous disks of the outer segments.", "contents": "Ionic aspects of excitation in rod outer segments. The current status of the problem of ionic mechanisms underlying excitation of vertebrate photoreceptors is reviewed. Evidence is presented that the ionic dark current of retinal rods is sustained by the action of a ouabain-sensitive Na-K exchange pump driven by oxidative metabolism. The photoreceptors are depleted of K when the pump is stopped by ouabain. Considerations of cell membrane topology, kinetics of the light response, and signal amplification indicate that the light-induced suppression of the ionic dark current is mediated by a diffusible internal chemical transmitter substance. The desensitizing effect of low CA2+ Ringer's on the light responses of vertebrate rods, along with other indirect evidence, suggests that the transmitter substance may be calcium ions released from the internal membranous disks of the outer segments."}
{"id": "PMID:1080106", "title": "Surface carbohydrate composition of different types of chicken lymphocytes.", "content": "A simple and quick procedure was used to analyse the carbohydrate composition of surface glycoproteins from chicken lymphocytes. The procedure included papain digestion of the cells, a two-step purification of the supernatant material and a sugar analysis by gaschromatography. The method made it possible to analyse samples of about 10(9) lymphocytes. The surface glycoproteins from different chicken lymphocyte preparations were found to differ significantly in their sugar composition. Lymphocytes from thymus, bursa, spleen or blood were characterized by typical relative amounts of glucose, galactosamine, fucose and sialic acid. The values for mannose, glucosamine and galactose, however, were approximately 1:1:1 for all four lymphocyte preparations. Bursectomy or thymectomy in combination with whole body irradiation altered the carbohydrate composition significantly. The results suggest the possibility that the carbohydrate composition can be used as a marker for different lymphocyte populations. The results are also discussed in respect to the hypothesis that carbohydrate determinants on the cell surface determine the migration of lymphocytes.", "contents": "Surface carbohydrate composition of different types of chicken lymphocytes. A simple and quick procedure was used to analyse the carbohydrate composition of surface glycoproteins from chicken lymphocytes. The procedure included papain digestion of the cells, a two-step purification of the supernatant material and a sugar analysis by gaschromatography. The method made it possible to analyse samples of about 10(9) lymphocytes. The surface glycoproteins from different chicken lymphocyte preparations were found to differ significantly in their sugar composition. Lymphocytes from thymus, bursa, spleen or blood were characterized by typical relative amounts of glucose, galactosamine, fucose and sialic acid. The values for mannose, glucosamine and galactose, however, were approximately 1:1:1 for all four lymphocyte preparations. Bursectomy or thymectomy in combination with whole body irradiation altered the carbohydrate composition significantly. The results suggest the possibility that the carbohydrate composition can be used as a marker for different lymphocyte populations. The results are also discussed in respect to the hypothesis that carbohydrate determinants on the cell surface determine the migration of lymphocytes."}
{"id": "PMID:1080107", "title": "Idiopathic orthostatic hypotension. Biochemical and pathologic observations in 2 cases.", "content": "Two cases of idiopathic orthostatic hypotension are reported, one associated with striato-nigral degeneration (case 1) and the other with Lewy body parkinsonism (case 2). In case 1, cerebrospinal fluid monoamine studies were carried out before and during treatment with 5-HTP and L-dopa. Low values of 5-HIAA appeared to be associated with degeneration of the spinal cord intermediolateral column. L-dopa had a beneficial effect on both parkinsonism and hypotension over a period of 6 months. New pathologic observations included changes in the sacral cord and a topographically related involvement of the substantia nigra and striatum, consistent with recent experimental neuroanatomical data.", "contents": "Idiopathic orthostatic hypotension. Biochemical and pathologic observations in 2 cases. Two cases of idiopathic orthostatic hypotension are reported, one associated with striato-nigral degeneration (case 1) and the other with Lewy body parkinsonism (case 2). In case 1, cerebrospinal fluid monoamine studies were carried out before and during treatment with 5-HTP and L-dopa. Low values of 5-HIAA appeared to be associated with degeneration of the spinal cord intermediolateral column. L-dopa had a beneficial effect on both parkinsonism and hypotension over a period of 6 months. New pathologic observations included changes in the sacral cord and a topographically related involvement of the substantia nigra and striatum, consistent with recent experimental neuroanatomical data."}
{"id": "PMID:1080108", "title": "Coronary arteriographic appearances in patients with left anterior hemiblock.", "content": "24 of 163 consecutive patients (14.7%) undergoing selective coronary arteriography were found to have electrocardiographic evidence of left anterior hemiblock. 3 patients (2 of whom had alcoholic cardiomyopathy) had normal coronary arteries, 15 patients had triple vessel disease, 3 patients had double vessel disease, and 3 patients had single vessel disease. 18 patients (75%) had moderate to severe left ventricular enlargement as estimated by left ventriculogram. Comparison of these angiographic findings with those of 88 patients with normal intraventricular conduction showed the distribution of the coronary artery disease to be essentially similar in the two groups but that significant left ventricular enlargement was at least three times more frequent in patients with left anterior hemiblock than in patients with normal intraventricular conduction. It is suggested that the development of left anterior hemiblock depends more upon the presence of left ventricular enlargement than on the distribution of the coronary artery disease.", "contents": "Coronary arteriographic appearances in patients with left anterior hemiblock. 24 of 163 consecutive patients (14.7%) undergoing selective coronary arteriography were found to have electrocardiographic evidence of left anterior hemiblock. 3 patients (2 of whom had alcoholic cardiomyopathy) had normal coronary arteries, 15 patients had triple vessel disease, 3 patients had double vessel disease, and 3 patients had single vessel disease. 18 patients (75%) had moderate to severe left ventricular enlargement as estimated by left ventriculogram. Comparison of these angiographic findings with those of 88 patients with normal intraventricular conduction showed the distribution of the coronary artery disease to be essentially similar in the two groups but that significant left ventricular enlargement was at least three times more frequent in patients with left anterior hemiblock than in patients with normal intraventricular conduction. It is suggested that the development of left anterior hemiblock depends more upon the presence of left ventricular enlargement than on the distribution of the coronary artery disease."}
{"id": "PMID:1080109", "title": "Mechanical and electrical causes for lack of frequency potentiation in normal patients, in coronary artery disease and in left bundle branch block.", "content": "Nine atrial pacing (SP) runs and 8 ventricular pacing (VP) runs were carried out in patients without heart disease, and 10 AP runs in patients with coronary artery disease (CAD). For evaluation of myocardial contractility, the time derivative of left ventricular pressure (dpdt) was used. Comparing rest and AP at 155/min, AP in normal patients revealed a significant increase in dP/dtmax and a significant decrease in left ventricular end-diastolic pressure (LVEDP). This represents frequency potentiation. When comparing rest and VP values at 155/min in normal patients, no changes in dP/dtmax and LVEDP were seen. AP in patients with CAD demonstrated no change in dP/dtmax, but a decrease in LVEDP when compared to the rest values. dP/dtmin and left ventricular systolic pressure did not change in the 3 groups. VP, and even AP, in 2 additional patients with isolated left bundle branch block (LBBB) AND WITHOUt associated heart disease, revealed the same constellation of parameters as VP in normal patients. Our results show a lack of frequency potentiation in normal patients during VP and in patients with isolated LBBB during both AP and VP. It is concluded that the lack of frequency potentiation during AP in CAD is produced primarily by mechanical asynchrony of contraction, angiographically demonstrated as akinesia and dyskinesia. The lack of frequency potentiation in normal patients during VP and in patients with isolated LBBB due to asynchrony of activation resulting in asynchrony of contraction.", "contents": "Mechanical and electrical causes for lack of frequency potentiation in normal patients, in coronary artery disease and in left bundle branch block. Nine atrial pacing (SP) runs and 8 ventricular pacing (VP) runs were carried out in patients without heart disease, and 10 AP runs in patients with coronary artery disease (CAD). For evaluation of myocardial contractility, the time derivative of left ventricular pressure (dpdt) was used. Comparing rest and AP at 155/min, AP in normal patients revealed a significant increase in dP/dtmax and a significant decrease in left ventricular end-diastolic pressure (LVEDP). This represents frequency potentiation. When comparing rest and VP values at 155/min in normal patients, no changes in dP/dtmax and LVEDP were seen. AP in patients with CAD demonstrated no change in dP/dtmax, but a decrease in LVEDP when compared to the rest values. dP/dtmin and left ventricular systolic pressure did not change in the 3 groups. VP, and even AP, in 2 additional patients with isolated left bundle branch block (LBBB) AND WITHOUt associated heart disease, revealed the same constellation of parameters as VP in normal patients. Our results show a lack of frequency potentiation in normal patients during VP and in patients with isolated LBBB during both AP and VP. It is concluded that the lack of frequency potentiation during AP in CAD is produced primarily by mechanical asynchrony of contraction, angiographically demonstrated as akinesia and dyskinesia. The lack of frequency potentiation in normal patients during VP and in patients with isolated LBBB due to asynchrony of activation resulting in asynchrony of contraction."}
{"id": "PMID:1080110", "title": "Unusual cholinergic response of bullfrog sympathetic ganglion cells.", "content": "The membrane of bullfrog sympathetic ganglion cells was hyperpolarized by a direct action of ACh (more than 0.5 mM) in a solution containing both nicotine (0.24 mM) and atropine (0.14 mM). This ACh hyperpolarization could be imitated by neither carbachol nor bethanechol, suggesting that the ACh hyperpolarization was the response which was produced by a specific action of ACh, which appeared to be neither nicotinic nor muscarinic. The size of ACh hyperpolarization was increased during a conditioning hyperpolarization. The ACh hyperpolarization was completely blocked by ouabain (2 times 10(-3) mM) and eliminated in the Na-free lithium solution. These aspects of the ACh hyperpolarization suggested that generation of this hyperpolarization was associated with the sodium pump. The ACh hyperpolarization seemed to be partially responsible for the production of the slow IPSP, since a part of the slow IPSP remained occasionally in the presence of both nicotine and atropine.", "contents": "Unusual cholinergic response of bullfrog sympathetic ganglion cells. The membrane of bullfrog sympathetic ganglion cells was hyperpolarized by a direct action of ACh (more than 0.5 mM) in a solution containing both nicotine (0.24 mM) and atropine (0.14 mM). This ACh hyperpolarization could be imitated by neither carbachol nor bethanechol, suggesting that the ACh hyperpolarization was the response which was produced by a specific action of ACh, which appeared to be neither nicotinic nor muscarinic. The size of ACh hyperpolarization was increased during a conditioning hyperpolarization. The ACh hyperpolarization was completely blocked by ouabain (2 times 10(-3) mM) and eliminated in the Na-free lithium solution. These aspects of the ACh hyperpolarization suggested that generation of this hyperpolarization was associated with the sodium pump. The ACh hyperpolarization seemed to be partially responsible for the production of the slow IPSP, since a part of the slow IPSP remained occasionally in the presence of both nicotine and atropine."}
{"id": "PMID:1080111", "title": "Effects of glutamate and glutamic acid diethyl ester on the lobster muscle fibre and the frog spinal cord.", "content": "The effect of bath applications of glutamic acid diethyl ester (GDE) and glutamate on the lobster muscle fibre and the from spinal cord were examined. In the lobster muscle fibre GDE (2 X 10(-3) M) did not antagonize the depolarizing action of glutamate (10(-4) M). In the frog spinal cord a small reduction in the excitatory effects of glutamate (10(-4)-10(-3) M) after GDE pretreatment was found only if the latter was given in very high doses (over 2 X 10(-3) M) which stimulated the neuronal firing. These observations show that GDE is not a specific antagonist of glutamate in these tissues.", "contents": "Effects of glutamate and glutamic acid diethyl ester on the lobster muscle fibre and the frog spinal cord. The effect of bath applications of glutamic acid diethyl ester (GDE) and glutamate on the lobster muscle fibre and the from spinal cord were examined. In the lobster muscle fibre GDE (2 X 10(-3) M) did not antagonize the depolarizing action of glutamate (10(-4) M). In the frog spinal cord a small reduction in the excitatory effects of glutamate (10(-4)-10(-3) M) after GDE pretreatment was found only if the latter was given in very high doses (over 2 X 10(-3) M) which stimulated the neuronal firing. These observations show that GDE is not a specific antagonist of glutamate in these tissues."}
{"id": "PMID:1080112", "title": "Hydro-osmotic activity of \"carba' analogues of oxytocin and [8-arginine] vasopressin on frog (Rana esculenta) bladder.", "content": "The biological potency of neurohypophysial hormone analogues in stimulating water transport across the frog bladder (Rana esculenta) was estimated from the pD2 (negative logarithm of the peptide concentration eliciting 50% of its maximal hydroosmotic effect) and from the maximal response. Most analogues elicited maximal responses similar to those of the natural hormones. Both replacement of sulfur by a methylene group in the disulfide bridge and omission of the terminal amino group in oxytocin reduced hydroosmotic activity; carba substitution in oxytocin led to a greater drop in pD2 than the same substitution in deamino-oxytocin. On the contrary, the effects of substituting phenylalanine for tyrosine and of omitting the amino group were almost additive. Elimination of the carboxyl-terminal tripeptide sequence considerably reduced hydroosmotic potency; pressinamide was inactive up to 3 mug/ml. The highly potent antidiuretic peptides, deamino-[D-Arg8]vasopressin and deamino-6-carba-]D-Arg8]vasopressin, were weakly active in the hydroosmotic assay.", "contents": "Hydro-osmotic activity of \"carba' analogues of oxytocin and [8-arginine] vasopressin on frog (Rana esculenta) bladder. The biological potency of neurohypophysial hormone analogues in stimulating water transport across the frog bladder (Rana esculenta) was estimated from the pD2 (negative logarithm of the peptide concentration eliciting 50% of its maximal hydroosmotic effect) and from the maximal response. Most analogues elicited maximal responses similar to those of the natural hormones. Both replacement of sulfur by a methylene group in the disulfide bridge and omission of the terminal amino group in oxytocin reduced hydroosmotic activity; carba substitution in oxytocin led to a greater drop in pD2 than the same substitution in deamino-oxytocin. On the contrary, the effects of substituting phenylalanine for tyrosine and of omitting the amino group were almost additive. Elimination of the carboxyl-terminal tripeptide sequence considerably reduced hydroosmotic potency; pressinamide was inactive up to 3 mug/ml. The highly potent antidiuretic peptides, deamino-[D-Arg8]vasopressin and deamino-6-carba-]D-Arg8]vasopressin, were weakly active in the hydroosmotic assay."}
{"id": "PMID:1080115", "title": "The origin of blood k-hydroxyindoleacetic acid following L-hydrazino-alpha-methyldopa (MK-486).", "content": "Blood 5-HIAA-14C levels derived from exogenous D,L-5-HTP-14C were investigated in normal, sham-operated and nephrectomized rats pretreated with MK-486 and probenecid (200 mg/kg i.p.). Blood 5-HIAA-14C levels decreased in nephrectomized rats following probenecid. Peripheral decarboxylase inhibition by MK-486 was enhanced by both probenecid and nephrectomy. Increased penetration of 5-HTP into brain occurred following probenecid. The results indicate that following MK-486 about 20% of the blood 5-HIAA-14C derived from 5-HTP-14C in rats originates in the CNS.", "contents": "The origin of blood k-hydroxyindoleacetic acid following L-hydrazino-alpha-methyldopa (MK-486). Blood 5-HIAA-14C levels derived from exogenous D,L-5-HTP-14C were investigated in normal, sham-operated and nephrectomized rats pretreated with MK-486 and probenecid (200 mg/kg i.p.). Blood 5-HIAA-14C levels decreased in nephrectomized rats following probenecid. Peripheral decarboxylase inhibition by MK-486 was enhanced by both probenecid and nephrectomy. Increased penetration of 5-HTP into brain occurred following probenecid. The results indicate that following MK-486 about 20% of the blood 5-HIAA-14C derived from 5-HTP-14C in rats originates in the CNS."}
{"id": "PMID:1080113", "title": "The chloride-dependent depression by GABA in the frog spinal cord.", "content": "Spontaneous activities from ventral and dorsal roots of the isolated perfused spinal cord of the bullfrog were inhibited by GABA. beta-Alanine showed a strong and glycine a weaker inhibitory effect. The inhibitory effect of GABA was markedly reduced in a chloride-free medium, whereas glycine and beta-alanine still showed an inhibitory effect similar to that seen in normal medium. Employing the sucrose-gap method, a marked depolarization in the dorsal root and a small but obvious hyperpolarization in the ventral root were found by the application of GABA. These results support the view that GABA is one of the transmitters involved in pre- and postsynaptic inhibition of the spinal cord. The postsynaptic hyperpolarizing effect of GABA on motoneurones would be caused directly through increased permeability of the membrane to chloride ion. The depolarizing effect of GABA on primary afferent terminals is discussed in connection with chloride dependency.", "contents": "The chloride-dependent depression by GABA in the frog spinal cord. Spontaneous activities from ventral and dorsal roots of the isolated perfused spinal cord of the bullfrog were inhibited by GABA. beta-Alanine showed a strong and glycine a weaker inhibitory effect. The inhibitory effect of GABA was markedly reduced in a chloride-free medium, whereas glycine and beta-alanine still showed an inhibitory effect similar to that seen in normal medium. Employing the sucrose-gap method, a marked depolarization in the dorsal root and a small but obvious hyperpolarization in the ventral root were found by the application of GABA. These results support the view that GABA is one of the transmitters involved in pre- and postsynaptic inhibition of the spinal cord. The postsynaptic hyperpolarizing effect of GABA on motoneurones would be caused directly through increased permeability of the membrane to chloride ion. The depolarizing effect of GABA on primary afferent terminals is discussed in connection with chloride dependency."}
{"id": "PMID:1080116", "title": "The origin of blood 5-hydroxyindoleacetic acid following L-hydrazine-alpha-methyldopa (MK-486).", "content": "Blood 5-HIAA-14C levels derived from exogenous D,L-5-HTP-14C were investigated in normal, sham-operated and nephrectomized rats pretreated with MK-486 and probenecid (200 mg/kg i.p.). Blood 5-HIAA-14C levels decreased in nephrectomized rats following probenecid. Peripheral decarboxylase inhibition by MK-486 was enhanced by both probenecid and nephrectomy. Increased penetration of 5-HTP into brain occurred following probenecid. Thr results indicate that the following MK-486 about 20% of the blood 5-HIAA-14C derived from 5-HTP-14C in rats originates in the CNS.", "contents": "The origin of blood 5-hydroxyindoleacetic acid following L-hydrazine-alpha-methyldopa (MK-486). Blood 5-HIAA-14C levels derived from exogenous D,L-5-HTP-14C were investigated in normal, sham-operated and nephrectomized rats pretreated with MK-486 and probenecid (200 mg/kg i.p.). Blood 5-HIAA-14C levels decreased in nephrectomized rats following probenecid. Peripheral decarboxylase inhibition by MK-486 was enhanced by both probenecid and nephrectomy. Increased penetration of 5-HTP into brain occurred following probenecid. Thr results indicate that the following MK-486 about 20% of the blood 5-HIAA-14C derived from 5-HTP-14C in rats originates in the CNS."}
{"id": "PMID:1080114", "title": "The effect of aprotinin on the harmine-induced tremor in lymphostatic encephalopathic and normal rats.", "content": "In normal and lymphostatic encephalopathic rats, aprotinin pretreatment alters the duration of the harmine tremor and the cerebral concentration of the alkaloid; low doses shorten the tremor and decrease the concentration, a high dose causes the opposite. Aprotinin may decrease or increase the blood-brain barrier permeability of harmine. Because the findings are similar in both groups of rats the blood-brain barrier for harmine is assumed to be intact in lymphostatic encephalopathy. However, sick rats possibly suffer from an impaired cerebral elimination of harmine.", "contents": "The effect of aprotinin on the harmine-induced tremor in lymphostatic encephalopathic and normal rats. In normal and lymphostatic encephalopathic rats, aprotinin pretreatment alters the duration of the harmine tremor and the cerebral concentration of the alkaloid; low doses shorten the tremor and decrease the concentration, a high dose causes the opposite. Aprotinin may decrease or increase the blood-brain barrier permeability of harmine. Because the findings are similar in both groups of rats the blood-brain barrier for harmine is assumed to be intact in lymphostatic encephalopathy. However, sick rats possibly suffer from an impaired cerebral elimination of harmine."}
{"id": "PMID:1080120", "title": "Serotonergic and cholinergic mechanisms during disruption of approach and avoidance behavior.", "content": "Injections of D,L-5-hydroxytryptophan (D,L-5-HTP) into pigeons and rats working on approach schedules produce a period of behavioral depression that is temporally correlated to increased levels of total serotonin (5-?HT) in the telencephalon and diencephalon. Administration of alpha-methyl-meta-tyrosine (alpha-MMT) also results in depressed responding; however, the temporal correlation is with decreased levels of total 5-HT in brain. Our hypothesis to explain these two apparent opposite biochemical states which result in similar behavioral disruptions is that in both cases more 5-HT is released within certain key serotonergic synapses mediating this behavior. Evidence from subcellular studies supports this concept. tnot only are the levels of 5-HT significantly higher in preparations of nerve endings isolated from the telencephalon and diencephalon of pigeons given injections of D,L-5-HTP, but in vitro studies also show that low concentrations of L-5-HTP significantly increased the release of radioactive 5-HT from serotonergic nerve endings. On the other hand, L-5-HTP in much higher concentrations had no effect on the release of labeled dopamine or norephinephrine. A major metabolite of alpha-MMT, alpha-methyl meta tyramine, also caused a significant increase in the release of labeled 5-?HT from similar preparations of nerve endings. Whereas serotonin appears to be involved in the disruption of approach behavior, another series of studies have indicated that acetylcholine may play a role in excitation during avoidance behavior. Behavioral excitation observed following administration of tetrabenazine 18 hr after iproniazid pretreatment to rats working on shock-avoidance schedules was temporally correlated with lowered levels of acetylcholine in the telencephalon. Pretreatment with 0.8 mg/kg of atropine blocked excitation whereas one-eight of this dose increased the duration. Excitation in these rats was shortened by 50% following bilateral septal lesions, which lowered brain acetylcholine levels. Mechanisms to explain these neurochemical correlates of behavior are discussed.", "contents": "Serotonergic and cholinergic mechanisms during disruption of approach and avoidance behavior. Injections of D,L-5-hydroxytryptophan (D,L-5-HTP) into pigeons and rats working on approach schedules produce a period of behavioral depression that is temporally correlated to increased levels of total serotonin (5-?HT) in the telencephalon and diencephalon. Administration of alpha-methyl-meta-tyrosine (alpha-MMT) also results in depressed responding; however, the temporal correlation is with decreased levels of total 5-HT in brain. Our hypothesis to explain these two apparent opposite biochemical states which result in similar behavioral disruptions is that in both cases more 5-HT is released within certain key serotonergic synapses mediating this behavior. Evidence from subcellular studies supports this concept. tnot only are the levels of 5-HT significantly higher in preparations of nerve endings isolated from the telencephalon and diencephalon of pigeons given injections of D,L-5-HTP, but in vitro studies also show that low concentrations of L-5-HTP significantly increased the release of radioactive 5-HT from serotonergic nerve endings. On the other hand, L-5-HTP in much higher concentrations had no effect on the release of labeled dopamine or norephinephrine. A major metabolite of alpha-MMT, alpha-methyl meta tyramine, also caused a significant increase in the release of labeled 5-?HT from similar preparations of nerve endings. Whereas serotonin appears to be involved in the disruption of approach behavior, another series of studies have indicated that acetylcholine may play a role in excitation during avoidance behavior. Behavioral excitation observed following administration of tetrabenazine 18 hr after iproniazid pretreatment to rats working on shock-avoidance schedules was temporally correlated with lowered levels of acetylcholine in the telencephalon. Pretreatment with 0.8 mg/kg of atropine blocked excitation whereas one-eight of this dose increased the duration. Excitation in these rats was shortened by 50% following bilateral septal lesions, which lowered brain acetylcholine levels. Mechanisms to explain these neurochemical correlates of behavior are discussed."}
{"id": "PMID:1080122", "title": "Cell-mediated immune reactivity to hepatitis B surface antigen in liver diseases.", "content": "The cellular immune reactivity to hepatitis B surface antigen (HBsAg) was studied in patients with liver diseases using a purified preparation of HBsAg and the leukocyte-migration agarose test. Inhibition of leukocyte migration by HBsAg was found in all 9 patients in the acute phase of viral hepatitis but not during convalescence. HBsAg inhibited migration of leukocytes in only 1 of 15 patients with chronic persistent hepatitis, in none of 16 with chronic periportal hepatitis, and in 2 of 21 with nonhepatic liver diseases. In normal control subjects inhibition of migration was found only in 1 physician exposed to HBsAg. Similar but not identical results were obtained with HBsAg-positive serum used as antigen. In patients with chronic hepatitis there was no correlation between reactivity to HBsAg and the presence of autoantibodies or anti-HBs. While further investigations using other preparations of antigens associated with HBV (e.g., hepatitis B core antigen) may better clarify the role of hepatitis B virus in some liver diseases, the present investigations suggest that a reactivity to HBsAg in patients with acute viral hepatitis may indicate clearing of the antigen, whereas in patients with chronic hepatitis it may reflect a state of specific tolerance to this antigen.", "contents": "Cell-mediated immune reactivity to hepatitis B surface antigen in liver diseases. The cellular immune reactivity to hepatitis B surface antigen (HBsAg) was studied in patients with liver diseases using a purified preparation of HBsAg and the leukocyte-migration agarose test. Inhibition of leukocyte migration by HBsAg was found in all 9 patients in the acute phase of viral hepatitis but not during convalescence. HBsAg inhibited migration of leukocytes in only 1 of 15 patients with chronic persistent hepatitis, in none of 16 with chronic periportal hepatitis, and in 2 of 21 with nonhepatic liver diseases. In normal control subjects inhibition of migration was found only in 1 physician exposed to HBsAg. Similar but not identical results were obtained with HBsAg-positive serum used as antigen. In patients with chronic hepatitis there was no correlation between reactivity to HBsAg and the presence of autoantibodies or anti-HBs. While further investigations using other preparations of antigens associated with HBV (e.g., hepatitis B core antigen) may better clarify the role of hepatitis B virus in some liver diseases, the present investigations suggest that a reactivity to HBsAg in patients with acute viral hepatitis may indicate clearing of the antigen, whereas in patients with chronic hepatitis it may reflect a state of specific tolerance to this antigen."}
{"id": "PMID:1080125", "title": "Cell-mediated immunity to hepatitis B surface antigen in blood donors with persistent antigenaemia.", "content": "Cellular immunity to the hepatitis B surface antigen (HBsAg) and a liver-specific lipoprotein was studied, using the leucocyte migration test, in 38 asymptomatic blood donors found to have HBsAg in the serum. Sensitization to HBsAg was found in 26% and was related to the presence of liver damage, being detected in 47% of those with elevated serum aspartate aminotransferase but in only 13% with normal enzyme levels. The frequency of sensitization to this antigen in those with chronic persistent or chronic aggressive hepatitis on biopsy was also higher than in those with unrelated or minimal changes. The findings using the liver-specific lipoprotein as antigen were similar and there was a correlation between the results obtained with this and the hapatitis B surface antigen. This study supports the hypothesis that a T-lymphocyte response to hepatitis B virus antigen can initiate an autoimmune reaction to antigens such as liver-specific lipoprotein on the hepatocyte surface, and that this reaction may be of importance in the production of chronic liver damage. In the absence of the T-cell response, the autoimmune reaction cannot occur and the virus is able to establish a harmless symbiotic union with the host.", "contents": "Cell-mediated immunity to hepatitis B surface antigen in blood donors with persistent antigenaemia. Cellular immunity to the hepatitis B surface antigen (HBsAg) and a liver-specific lipoprotein was studied, using the leucocyte migration test, in 38 asymptomatic blood donors found to have HBsAg in the serum. Sensitization to HBsAg was found in 26% and was related to the presence of liver damage, being detected in 47% of those with elevated serum aspartate aminotransferase but in only 13% with normal enzyme levels. The frequency of sensitization to this antigen in those with chronic persistent or chronic aggressive hepatitis on biopsy was also higher than in those with unrelated or minimal changes. The findings using the liver-specific lipoprotein as antigen were similar and there was a correlation between the results obtained with this and the hapatitis B surface antigen. This study supports the hypothesis that a T-lymphocyte response to hepatitis B virus antigen can initiate an autoimmune reaction to antigens such as liver-specific lipoprotein on the hepatocyte surface, and that this reaction may be of importance in the production of chronic liver damage. In the absence of the T-cell response, the autoimmune reaction cannot occur and the virus is able to establish a harmless symbiotic union with the host."}
{"id": "PMID:1080129", "title": "Independent appearance of anti-thymocyte and anti-RNA antibodies in NZB/NZW F1 mice.", "content": "NZB and NZB/NZW (B/W) mice were studied for natural thymocytoxic autoantibody(NTA) and antibody to reovirus RNA by cytotoxicity and radioimmunoassay respectively.NZB mice developed NTA by 2-3 months of age.  RNA antibodies appeared later if atall.  NTA and anti-RNA were present in B/W mice starting at 4-5 months and increased in frequency with age.  Either activity could appear first and could be present without the other.  Thus, all four possible patterns of autoantibody occurrence were found.  NTA and anti-RNA in 12-month B/W sera were separable by sucrose density gradient ultracentrifugation; NTA is a 19S IgM antibody whereas anti-RNA is 7S.", "contents": "Independent appearance of anti-thymocyte and anti-RNA antibodies in NZB/NZW F1 mice. NZB and NZB/NZW (B/W) mice were studied for natural thymocytoxic autoantibody(NTA) and antibody to reovirus RNA by cytotoxicity and radioimmunoassay respectively.NZB mice developed NTA by 2-3 months of age.  RNA antibodies appeared later if atall.  NTA and anti-RNA were present in B/W mice starting at 4-5 months and increased in frequency with age.  Either activity could appear first and could be present without the other.  Thus, all four possible patterns of autoantibody occurrence were found.  NTA and anti-RNA in 12-month B/W sera were separable by sucrose density gradient ultracentrifugation; NTA is a 19S IgM antibody whereas anti-RNA is 7S."}
{"id": "PMID:1080130", "title": "Mechanisms of corticosteroid action on lymphocyte subpopulations.  I. Redistribution of circulating T and b lymphocytes to the bone marrow.", "content": "The effect of corticosteroid administration on the redistribution of sirculating lymphocytes was studied in the guinea-pig, since this species closely resembles man in its relative resistance to the lymphopenic effect of corticosteroids.  A single intravenous injection of hydrocortisone (either 10 mg or 100 mg/kg) caused a profound but transient lymphocytopenia which was maximal at 4 hours following injection, with a returnto normal counts by 24 hours.  There was a proportionately greater decrease in circulating T lymphocytes compared to B lymphocytes, although both populations were diminished.  Chronic cortisone acetate treatment (100 mg/kg subcutaneously for 7 days) caused a similiar pattern of lymphocytopenia except that it was sustained during the period of chronically elevated plasma cortisol levels. The lymphocytes remaining inthe circulation during the period of lymphocytopenia responded normally in vitro to the mitogens phytohemagglutinin, concanavalin A, and pokeweek mitogen.  There was very littleeffect of corticosteroid administration on the numbers, proportions, or mitogenic response of splenic lymphocytes.  There was a dramatic increase in the bone marrow of proportions and absolute numbers of lymphocytes bearing surface T-and B-cell markers, as well as a marked increase in response of bone marrow lymphocytes to mitogenic stimulation during the period of maximal circulating lymphocytopenia caused by the administration of corticosteroids, especially chronic cortisone acetate.  There was a preferential homing of reinfused -51Cr-labelled syngeneic peripheral blood lymphocytes to the bone marrow of corticosteroid-treated recipients.  These studies demonstrate aredistribution of circulating lymphocytes to the bone marrow during corticosteroid treatment, resulting in an increase in immunocompetence of this compartment, while the peripheral blood lymphocyte compartment is quantitatively immunosuppressed due to a lymphocytopenia.", "contents": "Mechanisms of corticosteroid action on lymphocyte subpopulations.  I. Redistribution of circulating T and b lymphocytes to the bone marrow. The effect of corticosteroid administration on the redistribution of sirculating lymphocytes was studied in the guinea-pig, since this species closely resembles man in its relative resistance to the lymphopenic effect of corticosteroids.  A single intravenous injection of hydrocortisone (either 10 mg or 100 mg/kg) caused a profound but transient lymphocytopenia which was maximal at 4 hours following injection, with a returnto normal counts by 24 hours.  There was a proportionately greater decrease in circulating T lymphocytes compared to B lymphocytes, although both populations were diminished.  Chronic cortisone acetate treatment (100 mg/kg subcutaneously for 7 days) caused a similiar pattern of lymphocytopenia except that it was sustained during the period of chronically elevated plasma cortisol levels. The lymphocytes remaining inthe circulation during the period of lymphocytopenia responded normally in vitro to the mitogens phytohemagglutinin, concanavalin A, and pokeweek mitogen.  There was very littleeffect of corticosteroid administration on the numbers, proportions, or mitogenic response of splenic lymphocytes.  There was a dramatic increase in the bone marrow of proportions and absolute numbers of lymphocytes bearing surface T-and B-cell markers, as well as a marked increase in response of bone marrow lymphocytes to mitogenic stimulation during the period of maximal circulating lymphocytopenia caused by the administration of corticosteroids, especially chronic cortisone acetate.  There was a preferential homing of reinfused -51Cr-labelled syngeneic peripheral blood lymphocytes to the bone marrow of corticosteroid-treated recipients.  These studies demonstrate aredistribution of circulating lymphocytes to the bone marrow during corticosteroid treatment, resulting in an increase in immunocompetence of this compartment, while the peripheral blood lymphocyte compartment is quantitatively immunosuppressed due to a lymphocytopenia."}
{"id": "PMID:1080131", "title": "The migratory behavior of T blasts to contact sensitivity reactions in activelyand passively sensitized mice.", "content": "The arrival of cells from lymph nodes immunized with the contact sensitizing agents oxazolone and picryl chloride at ears challenged with these antigens was studied inmice using the technique of labelling with -51Cr.  An apparent specificity of arrival was seen because the immune cells transfered contact sensitivity passively, giving rise to an inflammatory response in the ear, to which a subpopulation of cells (T blasts) was non-specifically attracted.  It was also shown that there are at least two distinct populations of cells with the ability to move to inflammatory sites: the first, found in immunized lymph nodes, moves to contact sensitivity reactions in both actively and passively sensitized mice; the second, found in bone marrow and oil-induced peritoneal exudates, moves to contact sensitivity reactions in actively sensitized mice, whereas in passively sensitized mice, the arrival of these cells at contact sensitivity reaction is poor.  It is suggested that the ability of T blasts to move to sites of inflammation my be useful as an assay technique for contact sensitivity reactions.", "contents": "The migratory behavior of T blasts to contact sensitivity reactions in activelyand passively sensitized mice. The arrival of cells from lymph nodes immunized with the contact sensitizing agents oxazolone and picryl chloride at ears challenged with these antigens was studied inmice using the technique of labelling with -51Cr.  An apparent specificity of arrival was seen because the immune cells transfered contact sensitivity passively, giving rise to an inflammatory response in the ear, to which a subpopulation of cells (T blasts) was non-specifically attracted.  It was also shown that there are at least two distinct populations of cells with the ability to move to inflammatory sites: the first, found in immunized lymph nodes, moves to contact sensitivity reactions in both actively and passively sensitized mice; the second, found in bone marrow and oil-induced peritoneal exudates, moves to contact sensitivity reactions in actively sensitized mice, whereas in passively sensitized mice, the arrival of these cells at contact sensitivity reaction is poor.  It is suggested that the ability of T blasts to move to sites of inflammation my be useful as an assay technique for contact sensitivity reactions."}
{"id": "PMID:1080132", "title": "Plasmid-linked ampicillin resistance in haempohilus influenza type b.", "content": "Four ampicillin-resistant, beta-lactamase-producing strains of Haempohilus influenzae type b were examined for the presence of plasmid deoxyribonucleic acid (DNA). Three resistant strains contained a 30 x 10-6-dalton (30Mdal) plasmid and one resitant strain contained a 3-Mdal plasmid. The ampicillin-sensitive Haemophilus strains examined did not contain plasmid DNA. Transformation of a sensitive H. influenzae strain to ampicillin resistance with isolated plasmid DNA preparations revealed that the structural gene for beta-lactamase resided on both plasmid species. DNA-DNA hybridization studies showed that the 30-Mdal Haemophilus plasmid contained the ampicillin translocation DNA segment (TnA) found on some R-factors of enteric origin of the H. influenzae plasmids.", "contents": "Plasmid-linked ampicillin resistance in haempohilus influenza type b. Four ampicillin-resistant, beta-lactamase-producing strains of Haempohilus influenzae type b were examined for the presence of plasmid deoxyribonucleic acid (DNA). Three resistant strains contained a 30 x 10-6-dalton (30Mdal) plasmid and one resitant strain contained a 3-Mdal plasmid. The ampicillin-sensitive Haemophilus strains examined did not contain plasmid DNA. Transformation of a sensitive H. influenzae strain to ampicillin resistance with isolated plasmid DNA preparations revealed that the structural gene for beta-lactamase resided on both plasmid species. DNA-DNA hybridization studies showed that the 30-Mdal Haemophilus plasmid contained the ampicillin translocation DNA segment (TnA) found on some R-factors of enteric origin of the H. influenzae plasmids."}
{"id": "PMID:1080133", "title": "Rosette formation of pig T lymphocytes with sheep erythrocytes.", "content": "The relationship of sheep RBC rosette formation to density of thymus and blood lymphocytes was investigated. Thymocyte density was unimodal and cells of all densities rosetted equally. Blood lymphocyte density was bimodal with most rosette-forming cells in the denser ficoll layers. Papain treatment of SRBC increases rosette formation with blood lymphocytes while apparently maintaining specificity of T cells.", "contents": "Rosette formation of pig T lymphocytes with sheep erythrocytes. The relationship of sheep RBC rosette formation to density of thymus and blood lymphocytes was investigated. Thymocyte density was unimodal and cells of all densities rosetted equally. Blood lymphocyte density was bimodal with most rosette-forming cells in the denser ficoll layers. Papain treatment of SRBC increases rosette formation with blood lymphocytes while apparently maintaining specificity of T cells."}
{"id": "PMID:1080134", "title": "Immune response to Trichinella spiralis in the rat. I. Development of cellular and humoral responses during chronic infection.", "content": "The immune response of rats to infection with Trichinella spiralis was studied serially for more than 1 year. Initial antigen-specific cellular reactivity, assessed by the lymphocyte transformation response, developed in the draining mesenteric nodes 3 days after infection. After 1 week reactive lymphocytes were detectable in the spleen and circulating blood, but the more 'remote' peripheral nodes did not harbor antigen-reactive cells until late in the second week. Thereafter, the patterns of antigen-responsiveness varied among the different lymphoid pools, but in all cases a decline in reactivity was seen after the first month. Serum hemagglutinating and homocytotropic antibodies, detectable by the tenth day, reached their peaks after 1 month of infection. Hemagglutinating titers persisted for more than 1 year but homocytotropic antibody was lost over this period. Comparisons are drawn between the evolution of the natural infection and the development of the host's immune response.", "contents": "Immune response to Trichinella spiralis in the rat. I. Development of cellular and humoral responses during chronic infection. The immune response of rats to infection with Trichinella spiralis was studied serially for more than 1 year. Initial antigen-specific cellular reactivity, assessed by the lymphocyte transformation response, developed in the draining mesenteric nodes 3 days after infection. After 1 week reactive lymphocytes were detectable in the spleen and circulating blood, but the more 'remote' peripheral nodes did not harbor antigen-reactive cells until late in the second week. Thereafter, the patterns of antigen-responsiveness varied among the different lymphoid pools, but in all cases a decline in reactivity was seen after the first month. Serum hemagglutinating and homocytotropic antibodies, detectable by the tenth day, reached their peaks after 1 month of infection. Hemagglutinating titers persisted for more than 1 year but homocytotropic antibody was lost over this period. Comparisons are drawn between the evolution of the natural infection and the development of the host's immune response."}
{"id": "PMID:1080135", "title": "The nature of the deoxyribonucleosides involved in the binding of carcinogenic hydrocarbons to the DNA of mouse embryo cells.", "content": "The DNA of mouse embryo cells was specifically labelled in the purine moieties with (G(3)H)-deoxyadenosine or in the cytosine moieties with (5-(3)H)-deoxycytidine. These cells were then treated with 7-methylbenz (a) anthracene (7MBA) or benzo (a)-pyrene (B(a)P) and the DNA isolated, degraded and fractionated by LH20 Sephadex column chromatography. When the purines of the DNA were tritium-labelled, radioactive hydroccarbondeoxyribonucleoside products were obtained. No such products were found with deoxycytidine pre-labelled DNA. Contrary to an earlier suggestion, these results indicate that it is the purine moieties of DNA which react with the metabolically activated hydrocarbon derivative in vivo.", "contents": "The nature of the deoxyribonucleosides involved in the binding of carcinogenic hydrocarbons to the DNA of mouse embryo cells. The DNA of mouse embryo cells was specifically labelled in the purine moieties with (G(3)H)-deoxyadenosine or in the cytosine moieties with (5-(3)H)-deoxycytidine. These cells were then treated with 7-methylbenz (a) anthracene (7MBA) or benzo (a)-pyrene (B(a)P) and the DNA isolated, degraded and fractionated by LH20 Sephadex column chromatography. When the purines of the DNA were tritium-labelled, radioactive hydroccarbondeoxyribonucleoside products were obtained. No such products were found with deoxycytidine pre-labelled DNA. Contrary to an earlier suggestion, these results indicate that it is the purine moieties of DNA which react with the metabolically activated hydrocarbon derivative in vivo."}
{"id": "PMID:1080137", "title": "Differential regeneration of intestinal proliferative cells and cryptogenic cells after irradiation.", "content": "A dose of 900 rad gamma-rays to mice reduces the number of cryptogenic cells (those capable of crypt regeneration) per small intestinal crypt from about 80 to about 2 per surviving crypt. The surviving cells repopulate the crypt, the estimated cell-number doubling-time being about 24 hours, and on the fourth day after irradiation their numbers are still slightly below normal. The regeneration patterns for cryptogenic and proliferative cells differ significantly over the first 4 days after irradiation. The numbers of proliferative cells fall initially and remain low while the cryptogenic cells are increasing in number. The low levels persist for the first 1-5--2 days, after which there is a rapid rise in proliferative cells per crypt with a significant overshoot on the third day and a peak on the fourth day. The number of proliferative cells per crypt in control animals is about twice the number of cryptogenic cells. This ratio is on average 12 after irradiation (days 1--4). These data add support to the hypothesis that the small intestinal crypts contain a sub-population of stem cells that are distinct from the proliferative cells.", "contents": "Differential regeneration of intestinal proliferative cells and cryptogenic cells after irradiation. A dose of 900 rad gamma-rays to mice reduces the number of cryptogenic cells (those capable of crypt regeneration) per small intestinal crypt from about 80 to about 2 per surviving crypt. The surviving cells repopulate the crypt, the estimated cell-number doubling-time being about 24 hours, and on the fourth day after irradiation their numbers are still slightly below normal. The regeneration patterns for cryptogenic and proliferative cells differ significantly over the first 4 days after irradiation. The numbers of proliferative cells fall initially and remain low while the cryptogenic cells are increasing in number. The low levels persist for the first 1-5--2 days, after which there is a rapid rise in proliferative cells per crypt with a significant overshoot on the third day and a peak on the fourth day. The number of proliferative cells per crypt in control animals is about twice the number of cryptogenic cells. This ratio is on average 12 after irradiation (days 1--4). These data add support to the hypothesis that the small intestinal crypts contain a sub-population of stem cells that are distinct from the proliferative cells."}
{"id": "PMID:1080138", "title": "Mechanisms of protection of gamma-irradiated bacteriophage lambda by proflavine.", "content": "The protective effect of proflavine on gamma-irradiated bacteriophage lambda and its isolated DNA was investigated under conditions of predominantly indirect or direct effects. In both conditions addition of small amounts of the dye during irradiation of phage or DNA was shown to enhance their biological activity. Protection against indirect effects results probably from extensive scavenging of radioinduced water radicals within the medium. On the other hand the results obtained at minus 196 degrees C, with irradiated DNA-proflavine complexes, imply the existence of a long-range transfer of the primary radiation damage of DNA towards the intercalated molecules of proflavine. A mechanism for the protective effect of proflavine against the direct effect of ionizing radiation on biologically active DNA is suggested.", "contents": "Mechanisms of protection of gamma-irradiated bacteriophage lambda by proflavine. The protective effect of proflavine on gamma-irradiated bacteriophage lambda and its isolated DNA was investigated under conditions of predominantly indirect or direct effects. In both conditions addition of small amounts of the dye during irradiation of phage or DNA was shown to enhance their biological activity. Protection against indirect effects results probably from extensive scavenging of radioinduced water radicals within the medium. On the other hand the results obtained at minus 196 degrees C, with irradiated DNA-proflavine complexes, imply the existence of a long-range transfer of the primary radiation damage of DNA towards the intercalated molecules of proflavine. A mechanism for the protective effect of proflavine against the direct effect of ionizing radiation on biologically active DNA is suggested."}
{"id": "PMID:1080139", "title": "Comparsion of the oxygen-enhancement ratio for gamma-ray-induced double-strand breaks in the DNA of bacteriophage T7 as determined by two different methods of analysis.", "content": "Bacteriophage T7 was irradiated in a protecting medium under nitrogen and oxygen with 60Co gamma-rays. Double-strand breaks were measured by sucrose-gradient sedimentation and by boundary-sedimentation analysis. Both methods showed that the presence of oxygen during irradiation enhanced the production of double-strand breaks. This is at variance with a recent report which suggests that boundary-sedimentation analysis does not show an effect of oxygen. The discrepancy must be ascribed to differences in interpretation of the sedimentation data.", "contents": "Comparsion of the oxygen-enhancement ratio for gamma-ray-induced double-strand breaks in the DNA of bacteriophage T7 as determined by two different methods of analysis. Bacteriophage T7 was irradiated in a protecting medium under nitrogen and oxygen with 60Co gamma-rays. Double-strand breaks were measured by sucrose-gradient sedimentation and by boundary-sedimentation analysis. Both methods showed that the presence of oxygen during irradiation enhanced the production of double-strand breaks. This is at variance with a recent report which suggests that boundary-sedimentation analysis does not show an effect of oxygen. The discrepancy must be ascribed to differences in interpretation of the sedimentation data."}
{"id": "PMID:1080140", "title": "Thermoluminescent properties of crystalline halogen substituted uracils.", "content": "The thermoluminescent properties of crystalline uracil (U), and its halogen-substituted derivatives, 5 bromo-uracil (5BrU), 5 fluorouracil (5FU) and 5 iodo-uracil (5IU), have been investigated. The X-ray-induced thermoluminescent output increases in the order U less than 5 FU less than 5BrU less than 5IU. However, a reversed relationship is found for the U.V.-stimulated thermoluminescence. The thermoluminescence-emission spectra suggest that the same de-excitation process operates in all the halogen-substituted uracils, but the glow curves show that the charge-trapping characteristics are different for each material.", "contents": "Thermoluminescent properties of crystalline halogen substituted uracils. The thermoluminescent properties of crystalline uracil (U), and its halogen-substituted derivatives, 5 bromo-uracil (5BrU), 5 fluorouracil (5FU) and 5 iodo-uracil (5IU), have been investigated. The X-ray-induced thermoluminescent output increases in the order U less than 5 FU less than 5BrU less than 5IU. However, a reversed relationship is found for the U.V.-stimulated thermoluminescence. The thermoluminescence-emission spectra suggest that the same de-excitation process operates in all the halogen-substituted uracils, but the glow curves show that the charge-trapping characteristics are different for each material."}
{"id": "PMID:1080141", "title": "Some aspects of brain neurochemistry after intrauterine exposure to tritium.", "content": "Rats were continuously exposed to tritiated water (0, 1, 10 and 100 muCi HTO/ml body water) from conception to birth. Calculated, cumulative whole-body doses to the embryo and foetus were approximately 0, 6-6, 66 and 660 rad. The levels of several enzymes and established or presumptive central nervous system neurotransmitters were examined postnatally in whole brains. These were norepinephrine (NE), dopamine (DA), acetylcholinesterase (AChE) and monoamine oxidase (MAO). Intrauterine exposures to doses as low as 66 rad produced measurable and persistent decreases in brain weight and increases in NE concentrations at 21 and 45 days postnatally. No differences from control values were seen in the rate of turnover of NE or the concentrations of DA, AChE or MAO at 45 days. Exposure in utero to 6-6 rad produced no detectable postnatal effects on the brain neurochemical parameters measured.", "contents": "Some aspects of brain neurochemistry after intrauterine exposure to tritium. Rats were continuously exposed to tritiated water (0, 1, 10 and 100 muCi HTO/ml body water) from conception to birth. Calculated, cumulative whole-body doses to the embryo and foetus were approximately 0, 6-6, 66 and 660 rad. The levels of several enzymes and established or presumptive central nervous system neurotransmitters were examined postnatally in whole brains. These were norepinephrine (NE), dopamine (DA), acetylcholinesterase (AChE) and monoamine oxidase (MAO). Intrauterine exposures to doses as low as 66 rad produced measurable and persistent decreases in brain weight and increases in NE concentrations at 21 and 45 days postnatally. No differences from control values were seen in the rate of turnover of NE or the concentrations of DA, AChE or MAO at 45 days. Exposure in utero to 6-6 rad produced no detectable postnatal effects on the brain neurochemical parameters measured."}
{"id": "PMID:1080142", "title": "Biological properties and response to x-rays of first-generation transplants of spontaneous mammary carcinomas in C3H mice.", "content": "First-generation transplants of spontaneous mouse mammary carcinomas have been used extensively for radiobiological investigations of fractionated irradiation schedules, r.b.e. of fast neutrons and effectiveness of radiosensitizers, as reported elsewhere. The present work investigates the growth characteristics of the tumours; the criteria for the choice of end-points used in the definition of 'local control' of irradiated tumours; the reason for a decrease of 30 per cent in X-ray dose required to control tumours in females as compared with male mice; the proportion of hypoxic cells and its variation with time (reoxygenation) after a single dose of 1500 rad of X-rays; and the repair capacity of tumour cells within 24 hours after a substantial first dose of X-rays. Evidence is presented that the male-female difference was due to a higher proportion of hypoxic cells in tumours in male than in female mice. The repair of sub-lethal injury in tumour cells made hypoxic was slightly less than in skin made hypoxic but not significantly so. In the two-dose experiments on clamped tumours, no evidence of induced synchrony was found.", "contents": "Biological properties and response to x-rays of first-generation transplants of spontaneous mammary carcinomas in C3H mice. First-generation transplants of spontaneous mouse mammary carcinomas have been used extensively for radiobiological investigations of fractionated irradiation schedules, r.b.e. of fast neutrons and effectiveness of radiosensitizers, as reported elsewhere. The present work investigates the growth characteristics of the tumours; the criteria for the choice of end-points used in the definition of 'local control' of irradiated tumours; the reason for a decrease of 30 per cent in X-ray dose required to control tumours in females as compared with male mice; the proportion of hypoxic cells and its variation with time (reoxygenation) after a single dose of 1500 rad of X-rays; and the repair capacity of tumour cells within 24 hours after a substantial first dose of X-rays. Evidence is presented that the male-female difference was due to a higher proportion of hypoxic cells in tumours in male than in female mice. The repair of sub-lethal injury in tumour cells made hypoxic was slightly less than in skin made hypoxic but not significantly so. In the two-dose experiments on clamped tumours, no evidence of induced synchrony was found."}
{"id": "PMID:1080146", "title": "Partial purification and properties of cathepsin D in the retinal pigment epithelium.", "content": "Cathespin D from the retinal pigment epithelium of bovine eyes was purified about 25-fold from a crude extract of retinal pigment epithelium by acid treatment, ammonium sulfate fractionation, and Sephadex G-200 column chromatography. The purified enzyme hydrolyzed bovine serum albumin optimally at pH values close to 4.0. Exposure of the enzyme to 60 degrees C. for 2 minutes resulted in 50 per cent inactivation of the activity. The enzyme activity was completely inhibited by 0.1 microgram per milliliter of pepstatin, slightly inhibited by trasylol, and not affected by soybean trypsin inhibitor. The apparent molecular weight of cathepsin D was estimated to be about 60,000 by gel filtration on Sephadex G-200.", "contents": "Partial purification and properties of cathepsin D in the retinal pigment epithelium. Cathespin D from the retinal pigment epithelium of bovine eyes was purified about 25-fold from a crude extract of retinal pigment epithelium by acid treatment, ammonium sulfate fractionation, and Sephadex G-200 column chromatography. The purified enzyme hydrolyzed bovine serum albumin optimally at pH values close to 4.0. Exposure of the enzyme to 60 degrees C. for 2 minutes resulted in 50 per cent inactivation of the activity. The enzyme activity was completely inhibited by 0.1 microgram per milliliter of pepstatin, slightly inhibited by trasylol, and not affected by soybean trypsin inhibitor. The apparent molecular weight of cathepsin D was estimated to be about 60,000 by gel filtration on Sephadex G-200."}
{"id": "PMID:1080147", "title": "Color vision: blue deficiencies in children?", "content": "Recent publicized reports based on the use of the Farnsworth Panel D-15 test suggest that a large percentage of young children have a deficiency of blue vision (tritan type). In our study, 413 school children (ages 3 to 10) were tested with both the Farnsworth Panel D-15 test, as well as the A.O. H-R-R plates. None of the children failed either test for blue-yellow vision when traditional scoring instructions were observed. As in previous reports, we find that the children make a number of minor errors which adults rarely make. These errors show marked age-related patterns, being more frequent in younger children. However, further analysis of these errors revealed that the relative frequency with which particular error types were made on the D-15 test was significantly correlated with the existing perceived color difference data for the visually normal adult population. In addition, retesting significantly reduced all error types and reversing the test sequence demonstrated that most of the minor errors were made in the last half of the test regardless of the color vision task. The overall increase in the number of minor test errors seen with young children seems unrelated to color defects. The modified scoring methods in conjunction with the characteristics of the Panel D-15 test design account for the high percentage of errors classified as errors of blue vision.", "contents": "Color vision: blue deficiencies in children? Recent publicized reports based on the use of the Farnsworth Panel D-15 test suggest that a large percentage of young children have a deficiency of blue vision (tritan type). In our study, 413 school children (ages 3 to 10) were tested with both the Farnsworth Panel D-15 test, as well as the A.O. H-R-R plates. None of the children failed either test for blue-yellow vision when traditional scoring instructions were observed. As in previous reports, we find that the children make a number of minor errors which adults rarely make. These errors show marked age-related patterns, being more frequent in younger children. However, further analysis of these errors revealed that the relative frequency with which particular error types were made on the D-15 test was significantly correlated with the existing perceived color difference data for the visually normal adult population. In addition, retesting significantly reduced all error types and reversing the test sequence demonstrated that most of the minor errors were made in the last half of the test regardless of the color vision task. The overall increase in the number of minor test errors seen with young children seems unrelated to color defects. The modified scoring methods in conjunction with the characteristics of the Panel D-15 test design account for the high percentage of errors classified as errors of blue vision."}
{"id": "PMID:1080148", "title": "Fate of transforming bacteriophage HP1 deoxyribonucleic acid in Haemophilus influenzae lysogens.", "content": "The biological fate of temperate phage HP1 deoxyribonucleic acid (DNA) was followed after uptake by defectively lysogenic competent Haemophilus influenzae cultures. The similar inactivation kinetics of three single phage genetic markers and of their triple combination indicated a complete rather than partial destruction of about half of the adsorbed DNA molecules. Intracellular DNA breakdown products were tentatively identified by hydroxyapatite column chromatography as short single strands and extensively damaged short double strands. Integrated donor DNA (after single-strand insertion?) was still highly efficient for triple-marker co-transformation. This suggests that whole or nearly whole donor DNA molecules were integrated. Some donor DNA was never integrated but remained largely unaltered. This DNA fraction did not contain significant amounts of recipient prophage marker activity. It is concluded that it had not participated in some kind of reciprocal recombination event involving the recipient chromosome. Since very similar phage DNA marker inactivation rates were observed after adsorption by competent nonlysogenic recipients (transfection), the relationship between biological inactivation of adsorbed donor phage DNA and its integration in lysogenic recipients is not clear.", "contents": "Fate of transforming bacteriophage HP1 deoxyribonucleic acid in Haemophilus influenzae lysogens. The biological fate of temperate phage HP1 deoxyribonucleic acid (DNA) was followed after uptake by defectively lysogenic competent Haemophilus influenzae cultures. The similar inactivation kinetics of three single phage genetic markers and of their triple combination indicated a complete rather than partial destruction of about half of the adsorbed DNA molecules. Intracellular DNA breakdown products were tentatively identified by hydroxyapatite column chromatography as short single strands and extensively damaged short double strands. Integrated donor DNA (after single-strand insertion?) was still highly efficient for triple-marker co-transformation. This suggests that whole or nearly whole donor DNA molecules were integrated. Some donor DNA was never integrated but remained largely unaltered. This DNA fraction did not contain significant amounts of recipient prophage marker activity. It is concluded that it had not participated in some kind of reciprocal recombination event involving the recipient chromosome. Since very similar phage DNA marker inactivation rates were observed after adsorption by competent nonlysogenic recipients (transfection), the relationship between biological inactivation of adsorbed donor phage DNA and its integration in lysogenic recipients is not clear."}
{"id": "PMID:1080149", "title": "Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae.", "content": "The deoxyribonucleic acid (DNA) of competent wild-type Haemophilus influenzae and rec1 mutant cells contains single-strand regions, as judged by alkaline sucrose sedimentation, benzoylated naphthoylated diethylaminoethyl-cellulose fractionation, and digestion with an enzyme specific for single-strand regions in DNA. In contrast, the DNA of competent rec2 cells does not contain single-strand regions. Since transforming DNA does not associate with recipient DNA in the rec2 mutant as it does in wild type and rec1, it is concluded that the single-strand regions in the DNA of the competent cells are important for an early step in recombination between cell DNA and transforming DNA.", "contents": "Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae. The deoxyribonucleic acid (DNA) of competent wild-type Haemophilus influenzae and rec1 mutant cells contains single-strand regions, as judged by alkaline sucrose sedimentation, benzoylated naphthoylated diethylaminoethyl-cellulose fractionation, and digestion with an enzyme specific for single-strand regions in DNA. In contrast, the DNA of competent rec2 cells does not contain single-strand regions. Since transforming DNA does not associate with recipient DNA in the rec2 mutant as it does in wild type and rec1, it is concluded that the single-strand regions in the DNA of the competent cells are important for an early step in recombination between cell DNA and transforming DNA."}
{"id": "PMID:1080150", "title": "Pediococcus cerevisiae mutant with altered transport of folates.", "content": "A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent process, and a concentrative uptake was also found in gluocse-starved cells and in cells incubated at OC. About 75% of the accumulated 5-CH3-H4PteGlu exchanged with amethopterin. In contrast to the wild type, the mutant accumulated both diastereoisomers of 5-CH3-H4PteGlue by glucose-dependent and glucose-independent processes. Amethopterin and PteGlue competitively inhibited the uptake in both processes, with an apparent lower affinity of the carrier for PteGlu than for the analogue. p-Chloromercuribenzoate strongly inhibited the uptake (75%). The p-chloromercuribenzoate-nonsusceptible and temperature-independent uptake was also competed by amethopterin. Metabolic poisons like sodium azide, potassium fluoride, iodoacetate, and 2,4-dimitrophenol inhibited the glucose-dependent process. Uptake, in the absence of glucose, was enhanced by sodium azide and potassium fluoride.", "contents": "Pediococcus cerevisiae mutant with altered transport of folates. A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent process, and a concentrative uptake was also found in gluocse-starved cells and in cells incubated at OC. About 75% of the accumulated 5-CH3-H4PteGlu exchanged with amethopterin. In contrast to the wild type, the mutant accumulated both diastereoisomers of 5-CH3-H4PteGlue by glucose-dependent and glucose-independent processes. Amethopterin and PteGlue competitively inhibited the uptake in both processes, with an apparent lower affinity of the carrier for PteGlu than for the analogue. p-Chloromercuribenzoate strongly inhibited the uptake (75%). The p-chloromercuribenzoate-nonsusceptible and temperature-independent uptake was also competed by amethopterin. Metabolic poisons like sodium azide, potassium fluoride, iodoacetate, and 2,4-dimitrophenol inhibited the glucose-dependent process. Uptake, in the absence of glucose, was enhanced by sodium azide and potassium fluoride."}
{"id": "PMID:1080151", "title": "Capsular polymer of Haemophilus influenzae, type b. I. Structural characterization of the capsular polymer of strain Eagan.", "content": "The capsular type-specific antigen of Haemophilus influenzae, type b, has been reported to have a unit structure composed of D-ribose and phosphate. Recently, the presence of ribitol was found in preparations of type b capsular antigen. Our analytical results show equimolar proportions of ribose, ribitol, and phosphate. Periodate oxidation studies, paper chromatography of acidic and alkaline hydrolysates, and NMR spectral data indicate the structure of the capular antigen of H. influenzae b, strain Eagan to be a polyribosylribitol phosphate polymer.", "contents": "Capsular polymer of Haemophilus influenzae, type b. I. Structural characterization of the capsular polymer of strain Eagan. The capsular type-specific antigen of Haemophilus influenzae, type b, has been reported to have a unit structure composed of D-ribose and phosphate. Recently, the presence of ribitol was found in preparations of type b capsular antigen. Our analytical results show equimolar proportions of ribose, ribitol, and phosphate. Periodate oxidation studies, paper chromatography of acidic and alkaline hydrolysates, and NMR spectral data indicate the structure of the capular antigen of H. influenzae b, strain Eagan to be a polyribosylribitol phosphate polymer."}
{"id": "PMID:1080152", "title": "The occurrence of actinlike filaments in association with migrating pigment granules in frog retinal pigment epithelium.", "content": "In the retina of the frog and certain other animals, melanin pigment granules move in response to light so as to shield photoreceptor outer segments. The granules are contained within the cells of the pigment epithelium (PE) which lie as a continuous sheet between the neural retina and the choroid. Moderate illumination of the eye causes the melanin granules to move from a region within a PE cell body into numerous fingerlike extensions of the cell which interdigitate with the receptor outer segments. This migration takes many minutes and is reversed when the light falling on the eye increases in intensity. Several reviews are concerned with the early descriptions of this phenomenon (6,30) and with more recent experiments (1,5,19). The mechanism of the pigment granule motion is undetermined although there are studies concerning PE ultrastructure (8, 23, 31), scanning electron microscopy of the fingerlike extensions of the PE cells (27), the role of the PE in photoreceptor phagocytosis (32), the nature of the pigment granules (19), and the action spectrum of the light which induces the migration (16). This study reports the presence of a system of microfilaments associated with the pigment granules in the fingerlike extensions processes of the PE cells. We demonstrate by heavy meromyosin (HMM) labeling that the filaments are actinlike in character and suggest that these filaments could be responsible for the migration of the melanin pigment granules.", "contents": "The occurrence of actinlike filaments in association with migrating pigment granules in frog retinal pigment epithelium. In the retina of the frog and certain other animals, melanin pigment granules move in response to light so as to shield photoreceptor outer segments. The granules are contained within the cells of the pigment epithelium (PE) which lie as a continuous sheet between the neural retina and the choroid. Moderate illumination of the eye causes the melanin granules to move from a region within a PE cell body into numerous fingerlike extensions of the cell which interdigitate with the receptor outer segments. This migration takes many minutes and is reversed when the light falling on the eye increases in intensity. Several reviews are concerned with the early descriptions of this phenomenon (6,30) and with more recent experiments (1,5,19). The mechanism of the pigment granule motion is undetermined although there are studies concerning PE ultrastructure (8, 23, 31), scanning electron microscopy of the fingerlike extensions of the PE cells (27), the role of the PE in photoreceptor phagocytosis (32), the nature of the pigment granules (19), and the action spectrum of the light which induces the migration (16). This study reports the presence of a system of microfilaments associated with the pigment granules in the fingerlike extensions processes of the PE cells. We demonstrate by heavy meromyosin (HMM) labeling that the filaments are actinlike in character and suggest that these filaments could be responsible for the migration of the melanin pigment granules."}
{"id": "PMID:1080153", "title": "On the connection between the transverse tubules and the plasma membrane in frog semitendinosus skeletal muscle. Are caveolae the mouths of the transverse tubule system?", "content": "The transverse tubular system (TTS) of skeletal muscle fibers represents the morphological basis for the inward spread of conduction of the electrical signal that triggers muscle contraction. A historical account of the main steps contributing to the elucidation of the structure and function of the TSS has been presented by Huxley (1971). While the localization of the TSS and its association with the sarcoplasmic reticulum (SR) is well documented; there is still a need further to develop our knowledge of the morphology of the connection between the TSS and the plasma membrane. It is generally believed that the TSS opens directly to the extracellular space and that there is continuity between its membrane and the sarcolemma. However, direct observation of such a connection has been clearly shown only for the myotome of fish (Franzini-Armstrong and Porter, 1964). In other muscle fibers, only indirect evidence of the connection has been provided by experiments showing penetration of extracellular tracers into the TSS. These extracellular markers were also observed inside another membrane-bounded compartment consisting of round profiles named \"caveolae\" (Yamada, 1955) or \"pinocytotic vesicles\" (Ashurst, 1969). The present study deals with the communication between the TTS, caveolae, and plasma membrane (Peachey, 1965); Ezerman and Ishikawa, 1967; Schiaffino and Margreth, 1968; and Rayns et al., 1968). A detailed study of the caveolae compartment was undertaken with ruthenium red as an electron-dense tracer. As a result of this study, we propose that in certain species the caveolae compartment represents the transitional region in the connection between the TSS and the sarcolemma.", "contents": "On the connection between the transverse tubules and the plasma membrane in frog semitendinosus skeletal muscle. Are caveolae the mouths of the transverse tubule system? The transverse tubular system (TTS) of skeletal muscle fibers represents the morphological basis for the inward spread of conduction of the electrical signal that triggers muscle contraction. A historical account of the main steps contributing to the elucidation of the structure and function of the TSS has been presented by Huxley (1971). While the localization of the TSS and its association with the sarcoplasmic reticulum (SR) is well documented; there is still a need further to develop our knowledge of the morphology of the connection between the TSS and the plasma membrane. It is generally believed that the TSS opens directly to the extracellular space and that there is continuity between its membrane and the sarcolemma. However, direct observation of such a connection has been clearly shown only for the myotome of fish (Franzini-Armstrong and Porter, 1964). In other muscle fibers, only indirect evidence of the connection has been provided by experiments showing penetration of extracellular tracers into the TSS. These extracellular markers were also observed inside another membrane-bounded compartment consisting of round profiles named \"caveolae\" (Yamada, 1955) or \"pinocytotic vesicles\" (Ashurst, 1969). The present study deals with the communication between the TTS, caveolae, and plasma membrane (Peachey, 1965); Ezerman and Ishikawa, 1967; Schiaffino and Margreth, 1968; and Rayns et al., 1968). A detailed study of the caveolae compartment was undertaken with ruthenium red as an electron-dense tracer. As a result of this study, we propose that in certain species the caveolae compartment represents the transitional region in the connection between the TSS and the sarcolemma."}
{"id": "PMID:1080154", "title": "[Hemobilia. (Apropos of 5 cases)].", "content": "Hemobilia or hemorrhage through the biliary tract is increasing in frequency. With spreading knowledge of the syndrome, the diagnosis is now rarely missed. There is also a real increase due to traffic accidents which often cause liver injuries. The typical triade with digestive hemorrhage, biliary colic and jaundice should always arouse suspicion of hemobilia. Hepatic angiography is the best and most accurate diagnostic method. Accidental or operative trauma constitute the major cause of macroscopic hemobilia where as microscopic hemobilia is a very frequent symptom in gallstone-disease. In this report five cases of different ethiology are described. Three were caused by abdominal trauma. The fourth case illustrates the risk of hemobilia from instrumental exploration of the common duct. Even when this is done carefully it might cause a hemorrhage with formation of obstructing clots. In the last patient there was severe hemobilia due to errosion of the cystic artery by a gallstone, penetrating into the duodenum. The treatment given in the five cases are examples of different methods which may be used, according to the nature of the lesion. In the three cases of traumatic hemobilia one healed spontaneously under angiographic control, one was cured by local hemostasis in the central liver rupture and the third had a successful hepatic resection. In the case with post-operative hemobilia, an obstructing clot had to be removed from the common duct. Simple cholecystectomy cured the patient with hemorrhage from the eroded cystic artery.", "contents": "[Hemobilia. (Apropos of 5 cases)]. Hemobilia or hemorrhage through the biliary tract is increasing in frequency. With spreading knowledge of the syndrome, the diagnosis is now rarely missed. There is also a real increase due to traffic accidents which often cause liver injuries. The typical triade with digestive hemorrhage, biliary colic and jaundice should always arouse suspicion of hemobilia. Hepatic angiography is the best and most accurate diagnostic method. Accidental or operative trauma constitute the major cause of macroscopic hemobilia where as microscopic hemobilia is a very frequent symptom in gallstone-disease. In this report five cases of different ethiology are described. Three were caused by abdominal trauma. The fourth case illustrates the risk of hemobilia from instrumental exploration of the common duct. Even when this is done carefully it might cause a hemorrhage with formation of obstructing clots. In the last patient there was severe hemobilia due to errosion of the cystic artery by a gallstone, penetrating into the duodenum. The treatment given in the five cases are examples of different methods which may be used, according to the nature of the lesion. In the three cases of traumatic hemobilia one healed spontaneously under angiographic control, one was cured by local hemostasis in the central liver rupture and the third had a successful hepatic resection. In the case with post-operative hemobilia, an obstructing clot had to be removed from the common duct. Simple cholecystectomy cured the patient with hemorrhage from the eroded cystic artery."}
{"id": "PMID:1080155", "title": "[Indications for portacaval anastomoses in portal hypertension due to alcoholic cirrhosis. (Role of anatomo-pathological examination.)].", "content": "The authors report their experience of 134 cases of porta-caval anastomosis for alcoholic cirrhosis. From their experience, they draw up the logical indications for this operation, i.e. in order to ensure better survival than that of patients treated in other ways. They suggest routine study of surgical biopsy, even as an emergency. This biopsy permits them to recognise which cases are liable to be progressive; in severe cases, surgery should not be undertaken. Thanks to this selection, the operative mortality is reduced to that of any other severe operation (6 p. 100) and later survival is improved owing to absence of recurrent hemorrhage.", "contents": "[Indications for portacaval anastomoses in portal hypertension due to alcoholic cirrhosis. (Role of anatomo-pathological examination.)]. The authors report their experience of 134 cases of porta-caval anastomosis for alcoholic cirrhosis. From their experience, they draw up the logical indications for this operation, i.e. in order to ensure better survival than that of patients treated in other ways. They suggest routine study of surgical biopsy, even as an emergency. This biopsy permits them to recognise which cases are liable to be progressive; in severe cases, surgery should not be undertaken. Thanks to this selection, the operative mortality is reduced to that of any other severe operation (6 p. 100) and later survival is improved owing to absence of recurrent hemorrhage."}
{"id": "PMID:1080156", "title": "Studies on the reproducibility of glass bead antibody affinity columns.", "content": "Rabbit antibovine spleen DNase II and antihuman parotid amylase IgG were coupled to zirconium-clad (MAO, Corning) or plain (GAO, Corning) glass beads. (These were challenged with DNase II or amylase (human parotid or pancreatic), respectively. Although columns of the beads bound the enzymes whereas control columns did not, recoveries from the immune adsorbents were not only not quantitative but extremely variable. The columns also shed material absorbing in the ultraviolet. The antiamylase IgG-MAO column bound amylase so firmly, yet without inactivating it, that an insoluble enzyme reactor was formed. For these experiments, amylase was labeled with 14C in vitro without loss of activity.", "contents": "Studies on the reproducibility of glass bead antibody affinity columns. Rabbit antibovine spleen DNase II and antihuman parotid amylase IgG were coupled to zirconium-clad (MAO, Corning) or plain (GAO, Corning) glass beads. (These were challenged with DNase II or amylase (human parotid or pancreatic), respectively. Although columns of the beads bound the enzymes whereas control columns did not, recoveries from the immune adsorbents were not only not quantitative but extremely variable. The columns also shed material absorbing in the ultraviolet. The antiamylase IgG-MAO column bound amylase so firmly, yet without inactivating it, that an insoluble enzyme reactor was formed. For these experiments, amylase was labeled with 14C in vitro without loss of activity."}
{"id": "PMID:1080157", "title": "Quantitative determination of indolic compounds in the rat brain using p-dimethylaminocinnamaldehyde as reagent.", "content": "A method is proposed for determining the metabolites of radioactive 5-hydroxytryptophan and of endogenous tryptophan in the rat brain. The compounds are separated by thin-layer chromatography and made visible with p-dimethylaminocinnamaldehyde (DACA) reagent. The conditions studied are those which allow the use of DACA reagent for the quantitative determination of tryptophan metabolites in brain tissue. Amount of 5 ng of serotonin and 20 ng of tryptophan can be determined by densitometry.", "contents": "Quantitative determination of indolic compounds in the rat brain using p-dimethylaminocinnamaldehyde as reagent. A method is proposed for determining the metabolites of radioactive 5-hydroxytryptophan and of endogenous tryptophan in the rat brain. The compounds are separated by thin-layer chromatography and made visible with p-dimethylaminocinnamaldehyde (DACA) reagent. The conditions studied are those which allow the use of DACA reagent for the quantitative determination of tryptophan metabolites in brain tissue. Amount of 5 ng of serotonin and 20 ng of tryptophan can be determined by densitometry."}
{"id": "PMID:1080158", "title": "Antiglobulins and glomerulonephritis. Classification of patients by the reactivity of their sera and renal tissue with aggregated and native human IgG.", "content": "Renal biopsies and sera from 41 consecutive patients were studied to determine if antiglobulins were found more frequently in patients with severely diseased glomeruli. Patients were classified into three groups: A, 12 patients with normal renal function and minimal histological evidence of glomerular disease; B, 18 patients with normal renal function but distinctly abnormal biopsies (16 cases) or proteinuria greater than 16 g/24 h (2 cases); and C, 11 patients with both decreased function and abnormal histology. Positive latex fixation tests for rheumatoid factor were found in none of group A, four (22%) of group B, and five (45%) of group C patients. Sera heated 56 degrees C for 30 min contained precipitins reactive with heat-aggregated IgG in none of seven group A, five of ten (50%) group B, and four of ten (40%) group C patients. The quantity of 135I-labeled patient globulin which bound to immunoadsorbents coated with Cohn fraction II in competition with an equal quantity of 131I-labeled globulin from pooled plasma of normal donors was also measured. Patient globulins bound in significantly greater quantity (greater than or equal 2 SD) than the control in none of the group A, 7 of 18 (39%) group B, and 7 of 11 (64%) group C patients. Renal biopsies from 18 patients were also studied for the ability to fix fluorescein-conjugated heat-aggregated and native human IgG. None of nine tissue specimens from group A or B patients fixed either fluorescein-conjugated protein whereas tissue from eight of nine group C patients showed glomerular localization of one or both reagents. Severity of disease as judged by renal function and glomerular histology correlated with the presence of tissue-fixed and serum antiglobulins. Thus, detection of antiglobulins in glomeruli and sera of patients with glomerulonephritis may indicate a relatively poor prognosis and raises the possibility that antiglobulins may be implicated in some way in the pathophysiology of human glomerulonephritis.", "contents": "Antiglobulins and glomerulonephritis. Classification of patients by the reactivity of their sera and renal tissue with aggregated and native human IgG. Renal biopsies and sera from 41 consecutive patients were studied to determine if antiglobulins were found more frequently in patients with severely diseased glomeruli. Patients were classified into three groups: A, 12 patients with normal renal function and minimal histological evidence of glomerular disease; B, 18 patients with normal renal function but distinctly abnormal biopsies (16 cases) or proteinuria greater than 16 g/24 h (2 cases); and C, 11 patients with both decreased function and abnormal histology. Positive latex fixation tests for rheumatoid factor were found in none of group A, four (22%) of group B, and five (45%) of group C patients. Sera heated 56 degrees C for 30 min contained precipitins reactive with heat-aggregated IgG in none of seven group A, five of ten (50%) group B, and four of ten (40%) group C patients. The quantity of 135I-labeled patient globulin which bound to immunoadsorbents coated with Cohn fraction II in competition with an equal quantity of 131I-labeled globulin from pooled plasma of normal donors was also measured. Patient globulins bound in significantly greater quantity (greater than or equal 2 SD) than the control in none of the group A, 7 of 18 (39%) group B, and 7 of 11 (64%) group C patients. Renal biopsies from 18 patients were also studied for the ability to fix fluorescein-conjugated heat-aggregated and native human IgG. None of nine tissue specimens from group A or B patients fixed either fluorescein-conjugated protein whereas tissue from eight of nine group C patients showed glomerular localization of one or both reagents. Severity of disease as judged by renal function and glomerular histology correlated with the presence of tissue-fixed and serum antiglobulins. Thus, detection of antiglobulins in glomeruli and sera of patients with glomerulonephritis may indicate a relatively poor prognosis and raises the possibility that antiglobulins may be implicated in some way in the pathophysiology of human glomerulonephritis."}
{"id": "PMID:1080159", "title": "Simple screening tests for the diagnosis of isolated clotting factor defects. With special reference to 'contact factor' defects.", "content": "Reagents may be prepared from normal plasma and used with the prothrombin time and partial thromboplastin time tests to distinguish isolated defects of factors I, II, VII, VIII, IX, X, XI, or XII.", "contents": "Simple screening tests for the diagnosis of isolated clotting factor defects. With special reference to 'contact factor' defects. Reagents may be prepared from normal plasma and used with the prothrombin time and partial thromboplastin time tests to distinguish isolated defects of factors I, II, VII, VIII, IX, X, XI, or XII."}
{"id": "PMID:1080160", "title": "Liver disease in infancy: histological features and relationship to alpha-antitrypsin phenotype.", "content": "Sixty-nine specimens of liver tissue from 53 infants with neonatal hepatitis or its sequelae were examined without knowledge of the alpha1-antitrypsin phenotype. Distinctive, diastase-resistant, PAS-positive, pure magenta-coloured, sharply defined globules, 2-20 microns in diameter were found in periportal and paraseptal hepatocytes in all liver biopsies from eight alpha1-antitrypsin deficient (PiZZ) infants biopsied after the age of 12 weeks. Such globules were not seen in biopsies from PiZZ individuals aged less than 12 weeks nor in individuals of normal alpha1-antitrypsin phenotype (PiMM). No other specific histological abnormality was found in PiZZ individuals, but in them giant-cell transformation was infrequent and liver damage was more severe, three of 14 cases developing cirrhosis in contrast to four of 27 PiMM subjects. The pathogenesis of liver disease in PiZZ individuals is discussed.", "contents": "Liver disease in infancy: histological features and relationship to alpha-antitrypsin phenotype. Sixty-nine specimens of liver tissue from 53 infants with neonatal hepatitis or its sequelae were examined without knowledge of the alpha1-antitrypsin phenotype. Distinctive, diastase-resistant, PAS-positive, pure magenta-coloured, sharply defined globules, 2-20 microns in diameter were found in periportal and paraseptal hepatocytes in all liver biopsies from eight alpha1-antitrypsin deficient (PiZZ) infants biopsied after the age of 12 weeks. Such globules were not seen in biopsies from PiZZ individuals aged less than 12 weeks nor in individuals of normal alpha1-antitrypsin phenotype (PiMM). No other specific histological abnormality was found in PiZZ individuals, but in them giant-cell transformation was infrequent and liver damage was more severe, three of 14 cases developing cirrhosis in contrast to four of 27 PiMM subjects. The pathogenesis of liver disease in PiZZ individuals is discussed."}
{"id": "PMID:1080161", "title": "A diallel cross analysis of genetic determinants of copulatory behavior in rats.", "content": "A dialled cross analysis was aimed at obtaining information regarding genetic influences on the copulatory behavior of rats, and permitting considerations of adaptive significance. Rats of 4 inbred strains, ACI, F344, LEW, and WF, and all possible F1 crosses were used. Of 385 males tested, 73 failed to mate. The F1 rats were more likely to mate than were rats with inbred genotypes. Overdominance was generally apparent for low frequencies of mounts and intromissions preceding ejaculation across 5 series and 3 tests. A trend for directional dominance toward low scores on various latency measures of copulatory behavior also was apparent. According to the theory relating directional dominance to adaptation, it would appear generally adaptive for rats to copulate rapidly and to ejaculate after relatively few mounts and intromissions.", "contents": "A diallel cross analysis of genetic determinants of copulatory behavior in rats. A dialled cross analysis was aimed at obtaining information regarding genetic influences on the copulatory behavior of rats, and permitting considerations of adaptive significance. Rats of 4 inbred strains, ACI, F344, LEW, and WF, and all possible F1 crosses were used. Of 385 males tested, 73 failed to mate. The F1 rats were more likely to mate than were rats with inbred genotypes. Overdominance was generally apparent for low frequencies of mounts and intromissions preceding ejaculation across 5 series and 3 tests. A trend for directional dominance toward low scores on various latency measures of copulatory behavior also was apparent. According to the theory relating directional dominance to adaptation, it would appear generally adaptive for rats to copulate rapidly and to ejaculate after relatively few mounts and intromissions."}
{"id": "PMID:1080162", "title": "The problem-oriented record: clinical application in a teaching hospital.", "content": "A dental team-care concept that incorporates the problem-oriented system has been instituted at The Medical College of Pennsylvania. The program, as described, is designed to achieve the following goals: 1) to enhance communication between doctors and auxiliaries and thus maintain continuity in patient care and improve the quality of care; 2) to identify all problems related to the care of a patient; 3) to evaluate the educational and technical ability of the residents and students; 4) to achieve an effective dental auxiliary utilization program; 5) to increase faculty involvement in the delivery of patient care; 6) to organize an effective system of peer review; 7) to maintain complete, accurate and systematic medical-dental records; and 8) to encourage an interdisciplinary approach to health care delivery.", "contents": "The problem-oriented record: clinical application in a teaching hospital. A dental team-care concept that incorporates the problem-oriented system has been instituted at The Medical College of Pennsylvania. The program, as described, is designed to achieve the following goals: 1) to enhance communication between doctors and auxiliaries and thus maintain continuity in patient care and improve the quality of care; 2) to identify all problems related to the care of a patient; 3) to evaluate the educational and technical ability of the residents and students; 4) to achieve an effective dental auxiliary utilization program; 5) to increase faculty involvement in the delivery of patient care; 6) to organize an effective system of peer review; 7) to maintain complete, accurate and systematic medical-dental records; and 8) to encourage an interdisciplinary approach to health care delivery."}
{"id": "PMID:1080163", "title": "Lymphokine production by C3b-stimulated B cells.", "content": "Trypsin cleavage of guinea pig C3 (60 sec, 20 degrees C) yields a fragment which stimulates guinea pig lymphocytes to produce a chemotactic lymphokine. These digested C3 preparations possess only minimal inherent chemotactic properties. The response is completely inhibited by prior adsorption of the trypsin-derived C3 fragments with insolubilized anti-C3. The active C3 fragment is C3b as demonstrated by the elution of activity in the exclusion volume of Sephadex G-75 (m.w. greater than 50,000 dalton). The C3b-dependent cellular-derived chemotactic factor is present in the supernatants of B cell cultures and totally absent in those of T cell cultures. Generation of this lymphokine occurs in the absence of cellular proliferation.", "contents": "Lymphokine production by C3b-stimulated B cells. Trypsin cleavage of guinea pig C3 (60 sec, 20 degrees C) yields a fragment which stimulates guinea pig lymphocytes to produce a chemotactic lymphokine. These digested C3 preparations possess only minimal inherent chemotactic properties. The response is completely inhibited by prior adsorption of the trypsin-derived C3 fragments with insolubilized anti-C3. The active C3 fragment is C3b as demonstrated by the elution of activity in the exclusion volume of Sephadex G-75 (m.w. greater than 50,000 dalton). The C3b-dependent cellular-derived chemotactic factor is present in the supernatants of B cell cultures and totally absent in those of T cell cultures. Generation of this lymphokine occurs in the absence of cellular proliferation."}
{"id": "PMID:1080164", "title": "Radiosensitivity of T and B lymphocytes. III. Effect of radiation on immunoglobulin production by B cells.", "content": "Radiation injury in defined populations of B cells was investigated utilizing an allotype congenic transfer system. The amount of donor immunoglobulin present in the recipient's serum was found to be directly proportional to the number of viable cells injected, and on this basis approximate D37 values for the inoculated B cells were determined in several experiments and found to fall in the 70 to 145 rad range depending upon the specific experimental conditions. Since the sensitivity of T cells to radiation-induced interphase death can be modified by prior exposure to select mitogens and antigens, an attempt was made to demonstrate a similar phenomenon with respect to B cells. The results indicate that the radiosensitivity of B cells can be slightly influenced by prior incubation with mitogens and only equivocally changed by activation with antigen.", "contents": "Radiosensitivity of T and B lymphocytes. III. Effect of radiation on immunoglobulin production by B cells. Radiation injury in defined populations of B cells was investigated utilizing an allotype congenic transfer system. The amount of donor immunoglobulin present in the recipient's serum was found to be directly proportional to the number of viable cells injected, and on this basis approximate D37 values for the inoculated B cells were determined in several experiments and found to fall in the 70 to 145 rad range depending upon the specific experimental conditions. Since the sensitivity of T cells to radiation-induced interphase death can be modified by prior exposure to select mitogens and antigens, an attempt was made to demonstrate a similar phenomenon with respect to B cells. The results indicate that the radiosensitivity of B cells can be slightly influenced by prior incubation with mitogens and only equivocally changed by activation with antigen."}
{"id": "PMID:1080165", "title": "Syngeneic adoptive immunotherapy and chemoimmunotherapy of a Friend leukemia: requirement for T cells.", "content": "The aim of the study was to determine which cell mediates adoptive immunotherapy and chemoimmunotherapy of a syngeneic transplantable Friend virus-induced leukemia (FBL-3). An adoptive immunotherapy model was developed in which adult C57BL/6 mice given a lethal dose (10(4)) of FBL-3 on day 0 were saved by treatment on day 1 with C57BL/6 spleen cells or peritoneal exudate cells (PEC) immune to FBL-3. Cells passed through a nylon wool column to remove B cells and macrophages or treated with carbonyl iron to remove phagocytic cells remained effective, whereas cells treated with anti-theta serum and complement were far less effective. For adoptive chemoimmunotherapy, mice inoculated with 10(7) FBL-3 were treated 5 days later with cyclophosphamide (CY) plus immune spleen cells. CY, with or without non-immune cells, prolonged survival but all mice died with leukemia, whereas mice given CY plus immune cells survived tumor-free. As an adjunct to CY, immune cells passed through nylon wool or treated with carbonyl iron remained quite effective whereas cells treated with anti-theta serum and complement were far less effective. Thus, immune thymus-derived lymphocytes were required for the adoptive immunotherapy of an early leukemia or chemoimmunotherapy of a disseminated leukemia.", "contents": "Syngeneic adoptive immunotherapy and chemoimmunotherapy of a Friend leukemia: requirement for T cells. The aim of the study was to determine which cell mediates adoptive immunotherapy and chemoimmunotherapy of a syngeneic transplantable Friend virus-induced leukemia (FBL-3). An adoptive immunotherapy model was developed in which adult C57BL/6 mice given a lethal dose (10(4)) of FBL-3 on day 0 were saved by treatment on day 1 with C57BL/6 spleen cells or peritoneal exudate cells (PEC) immune to FBL-3. Cells passed through a nylon wool column to remove B cells and macrophages or treated with carbonyl iron to remove phagocytic cells remained effective, whereas cells treated with anti-theta serum and complement were far less effective. For adoptive chemoimmunotherapy, mice inoculated with 10(7) FBL-3 were treated 5 days later with cyclophosphamide (CY) plus immune spleen cells. CY, with or without non-immune cells, prolonged survival but all mice died with leukemia, whereas mice given CY plus immune cells survived tumor-free. As an adjunct to CY, immune cells passed through nylon wool or treated with carbonyl iron remained quite effective whereas cells treated with anti-theta serum and complement were far less effective. Thus, immune thymus-derived lymphocytes were required for the adoptive immunotherapy of an early leukemia or chemoimmunotherapy of a disseminated leukemia."}
{"id": "PMID:1080166", "title": "Evidence for cell-receptor activity in lymphocyte stimulation by staphylococcal enterotoxin.", "content": "A wide dose-response curve and the inhibitory effect on mitogenicity of specific antitoxin suggest that polyclonal lymphocyte activation by staphylococcal enterotoxin requires direct interaction of toxin with lymphocyte receptors of low avidity for the protein. Staphylococcal enterotoxins A, B, and C1 demonstrated equivalent mitogenic activity. Lymphocyte receptors involved in enterotoxin activation thus appear to be specific for nonantigenic regions of the toxin molecule. Monosaccharide (hapten) inhibiton data indicate that lymphocyte receptors for staphylococcal enterotoxin lack alpha-mannoside, galactose, acetylgalactosamine, acetylglucosamine, and fucose (or closely related saccharides) as determinant sugars and thus differ significantly in structure from lectin cell receptors.", "contents": "Evidence for cell-receptor activity in lymphocyte stimulation by staphylococcal enterotoxin. A wide dose-response curve and the inhibitory effect on mitogenicity of specific antitoxin suggest that polyclonal lymphocyte activation by staphylococcal enterotoxin requires direct interaction of toxin with lymphocyte receptors of low avidity for the protein. Staphylococcal enterotoxins A, B, and C1 demonstrated equivalent mitogenic activity. Lymphocyte receptors involved in enterotoxin activation thus appear to be specific for nonantigenic regions of the toxin molecule. Monosaccharide (hapten) inhibiton data indicate that lymphocyte receptors for staphylococcal enterotoxin lack alpha-mannoside, galactose, acetylgalactosamine, acetylglucosamine, and fucose (or closely related saccharides) as determinant sugars and thus differ significantly in structure from lectin cell receptors."}
{"id": "PMID:1080167", "title": "Characteristics of mesenteric lymph node cells homing to gut-associated lymphoid tissue in syngeneic mice.", "content": "A subpopulation of cells in murine mesenteric lymph nodes, about 15% of those synthesizing DNA at any given time, homes specifically to the gut and mesenteric nodes of syngeneic recipients within 1 day of i.v. transfer. In contrast, cells from Oeyer's patches or peripheral lymph nodes do not. A large proportion of the B blasts which home to the small intestine has surface Ig, but lacks complement receptors. Thy-1-positive T blasts home to the gut to a lesser extent than B blasts. However, it is probable that equal fractions of B and T blasts home to mesenteric nodes. Homing is not affected by measures calculated to interfere with the combination of cell surface IgA and secretory component.", "contents": "Characteristics of mesenteric lymph node cells homing to gut-associated lymphoid tissue in syngeneic mice. A subpopulation of cells in murine mesenteric lymph nodes, about 15% of those synthesizing DNA at any given time, homes specifically to the gut and mesenteric nodes of syngeneic recipients within 1 day of i.v. transfer. In contrast, cells from Oeyer's patches or peripheral lymph nodes do not. A large proportion of the B blasts which home to the small intestine has surface Ig, but lacks complement receptors. Thy-1-positive T blasts home to the gut to a lesser extent than B blasts. However, it is probable that equal fractions of B and T blasts home to mesenteric nodes. Homing is not affected by measures calculated to interfere with the combination of cell surface IgA and secretory component."}
{"id": "PMID:1080168", "title": "Human lymphocyte subpopulation: early and late rosettes.", "content": "In the process of forming SRBC rosettes of human lymphocytes, two types were distinguished, the early and late rosettes. The former appeared immediately whereas the latter required further incubation at 4degreesC. In this study, the two types of cells were separated by gradient centrifugation and studied separately. It was found that the proportion of caps in the late rosettes was much greater than in the early ones. Treatment of the former by trypsin, AET, and neuraminidase decreased this proportion so that it resembled the late one. It was postulated that some of the differences in the early and late RFC lie in the differences in the content of membrane sulphydryl and sialic acid groups and distribution of SRBC receptors.", "contents": "Human lymphocyte subpopulation: early and late rosettes. In the process of forming SRBC rosettes of human lymphocytes, two types were distinguished, the early and late rosettes. The former appeared immediately whereas the latter required further incubation at 4degreesC. In this study, the two types of cells were separated by gradient centrifugation and studied separately. It was found that the proportion of caps in the late rosettes was much greater than in the early ones. Treatment of the former by trypsin, AET, and neuraminidase decreased this proportion so that it resembled the late one. It was postulated that some of the differences in the early and late RFC lie in the differences in the content of membrane sulphydryl and sialic acid groups and distribution of SRBC receptors."}
{"id": "PMID:1080169", "title": "A method for detection of Ia antigens in the absence of appropriate H-2 recombinants.", "content": "A method is described which obviates the use of H-2 recombinant strains for production and detection of Ia antibodies. The antibodies are produced by immunization with spleen cells in a strain combination in which the donor differs from the recipient either in the K end of the H-2 complex or in the whole complex. If the right schedule is used, this immunization produces antibodies against both H-2 and Ia antigens. The H-2 antibodies are absorbed out with erythrocytes or T lymphocytes rendering the antiserum specific for Ia antigens. The presence of Ia Ia antibodies is asserted by determining the tissue distribution of the antigens detected with the absorbed antiserum. Another antiserum is then prepared in the same strain combination by immunization with tissue that does not contain Ia but does contain H-2 antigens (e.g., a sarcoma of the proper genotype). The second antiserum contains only H-2 antibodies and the presence of these antibodies is again asserted by determining the tissue distribution of the corresponding antigens. The molecular distinctiveness of the putative Ia and H-2 antigens is then demonstrated by the newly developed technique of antibody-mediated induction of resistance to cytotoxicity (lysis). If the antigens detected with the two antisera move independently in the cell membrane, and if the antigens detected with the first antiserum do indeed have Ia-like properties, it is concluded that the antiserum detects Ia antigens. This method should prove to be useful for detection of Ia antigens in H-2 haplotypes for which no intra-H-2 recombinants are known, and for detection of Ia-like antigens in other mammalian species, particularly in man.", "contents": "A method for detection of Ia antigens in the absence of appropriate H-2 recombinants. A method is described which obviates the use of H-2 recombinant strains for production and detection of Ia antibodies. The antibodies are produced by immunization with spleen cells in a strain combination in which the donor differs from the recipient either in the K end of the H-2 complex or in the whole complex. If the right schedule is used, this immunization produces antibodies against both H-2 and Ia antigens. The H-2 antibodies are absorbed out with erythrocytes or T lymphocytes rendering the antiserum specific for Ia antigens. The presence of Ia Ia antibodies is asserted by determining the tissue distribution of the antigens detected with the absorbed antiserum. Another antiserum is then prepared in the same strain combination by immunization with tissue that does not contain Ia but does contain H-2 antigens (e.g., a sarcoma of the proper genotype). The second antiserum contains only H-2 antibodies and the presence of these antibodies is again asserted by determining the tissue distribution of the corresponding antigens. The molecular distinctiveness of the putative Ia and H-2 antigens is then demonstrated by the newly developed technique of antibody-mediated induction of resistance to cytotoxicity (lysis). If the antigens detected with the two antisera move independently in the cell membrane, and if the antigens detected with the first antiserum do indeed have Ia-like properties, it is concluded that the antiserum detects Ia antigens. This method should prove to be useful for detection of Ia antigens in H-2 haplotypes for which no intra-H-2 recombinants are known, and for detection of Ia-like antigens in other mammalian species, particularly in man."}
{"id": "PMID:1080170", "title": "T cell products activating stem cells: further studies on the origin and action of the factor(s).", "content": "We studied the capacity of bone marrow hemopoietic stem cells to form colonies (colony forming units, CFU) in the spleens of irradiated recipient mice following incubation of the cells with T cell-derived mediators in short-term culture in vitro. The mediators were a) stem cell-activating factor (SAF) present in crude form in the supernatant from phytohemagglutinin (PHA); stimulated lymphocytes, and b) immunoenhancing factor (IEF) obtained in partially purified form from the supernatant of antigen-stimulated lymphocytes and characterized by its ability to enhance antibody formation in vitro. Both SAF and IEF increased the number of CFU in the cultured bone marrow cell suspension. However, only SAF, and not IEF, significantly stimulated DNA synthesis in the cultured cells, Furhter more, SAF appears to activate CFU in vitro, rather than merely to promote the survival of active CFU stem cells. Experiments with SAF and bone marrow from different strains of mice indicated that the biologic activity was not restricted by K and Ia regions of H complex.", "contents": "T cell products activating stem cells: further studies on the origin and action of the factor(s). We studied the capacity of bone marrow hemopoietic stem cells to form colonies (colony forming units, CFU) in the spleens of irradiated recipient mice following incubation of the cells with T cell-derived mediators in short-term culture in vitro. The mediators were a) stem cell-activating factor (SAF) present in crude form in the supernatant from phytohemagglutinin (PHA); stimulated lymphocytes, and b) immunoenhancing factor (IEF) obtained in partially purified form from the supernatant of antigen-stimulated lymphocytes and characterized by its ability to enhance antibody formation in vitro. Both SAF and IEF increased the number of CFU in the cultured bone marrow cell suspension. However, only SAF, and not IEF, significantly stimulated DNA synthesis in the cultured cells, Furhter more, SAF appears to activate CFU in vitro, rather than merely to promote the survival of active CFU stem cells. Experiments with SAF and bone marrow from different strains of mice indicated that the biologic activity was not restricted by K and Ia regions of H complex."}
{"id": "PMID:1080171", "title": "Interaction between lymphocytes and inflammatory exudate cells. I. Enhacement of thymocyte response to PHA by product(s) of polymorphonuclear leukocytes and macrophages.", "content": "Thymocytes of C3H/He mice were poorly triggered to induce DNA synthesis by phytohemagglutinin but fairly well triggered when they were mixed with small number oble product(s) of these cells. Peritoneal exudate cells were mainly harvested at 3 hr, largely consisting of macrophages, after intraperitoneal injection of 0.2% sodium caseinate. Addition of 2(2) to 2(6) x 10(3) of 3-hr exudate cells, 2(2) to 2(4) x 10(3) of 48-hr exudate cells, or 2(2) to 2(4) x 10(3) of 96-hr exudate cells to syngeneic thymocyte culture (3 x 10(6) resulted in marked enhancement of DNA synthesis by thymocytes stimulated with 1 mul PHA. Similar enhancement of DNA synthesis was also observed in the presence of 200 mul of cellfree supernatant fluid of peritoneal exudates of 3 hr or 9 hr after casein stimulation. Conversely, two hundred microliters of 0-hr, 48-hr, or 96-hr peritoneal fluid showed a slight suppressing effect on thymocyte response and 50 to 100 mul of these exudates showed no enhancing effect. Furthermore, 50 to 800 mul of culture supernatants from 1.6 x 10(6) cells/4 ml of 3-hr exudate cells, 96-hr exudate cells, or purified macrophages also caused a similar enhancement of DNA synthesis by thymocytes. On the other hand, excess numbers of exudate cells (2(7) to 2(8) x 10(3) of 3-hr exudate cells, or 2(5) to 2(9) of 96-hr exudate cells) or excess amounts of culture supernatants (800 mul from 6.4 to 12.8 x 10(6)/4 ml of 3-hr cells, or 200 mul from 12.8 x 10(6)/4 ml, 400 mul from 6.4 x 10(6)/4ml, or 800 mul from 3.2 x 10(6)/4 ml of 96-hr cells) diminished the enhancing potency on thymocytes. The cooperative function by polymorphonuclear leukocytes or by macrophages on the lymphocyte response was thus postulated.", "contents": "Interaction between lymphocytes and inflammatory exudate cells. I. Enhacement of thymocyte response to PHA by product(s) of polymorphonuclear leukocytes and macrophages. Thymocytes of C3H/He mice were poorly triggered to induce DNA synthesis by phytohemagglutinin but fairly well triggered when they were mixed with small number oble product(s) of these cells. Peritoneal exudate cells were mainly harvested at 3 hr, largely consisting of macrophages, after intraperitoneal injection of 0.2% sodium caseinate. Addition of 2(2) to 2(6) x 10(3) of 3-hr exudate cells, 2(2) to 2(4) x 10(3) of 48-hr exudate cells, or 2(2) to 2(4) x 10(3) of 96-hr exudate cells to syngeneic thymocyte culture (3 x 10(6) resulted in marked enhancement of DNA synthesis by thymocytes stimulated with 1 mul PHA. Similar enhancement of DNA synthesis was also observed in the presence of 200 mul of cellfree supernatant fluid of peritoneal exudates of 3 hr or 9 hr after casein stimulation. Conversely, two hundred microliters of 0-hr, 48-hr, or 96-hr peritoneal fluid showed a slight suppressing effect on thymocyte response and 50 to 100 mul of these exudates showed no enhancing effect. Furthermore, 50 to 800 mul of culture supernatants from 1.6 x 10(6) cells/4 ml of 3-hr exudate cells, 96-hr exudate cells, or purified macrophages also caused a similar enhancement of DNA synthesis by thymocytes. On the other hand, excess numbers of exudate cells (2(7) to 2(8) x 10(3) of 3-hr exudate cells, or 2(5) to 2(9) of 96-hr exudate cells) or excess amounts of culture supernatants (800 mul from 6.4 to 12.8 x 10(6)/4 ml of 3-hr cells, or 200 mul from 12.8 x 10(6)/4 ml, 400 mul from 6.4 x 10(6)/4ml, or 800 mul from 3.2 x 10(6)/4 ml of 96-hr cells) diminished the enhancing potency on thymocytes. The cooperative function by polymorphonuclear leukocytes or by macrophages on the lymphocyte response was thus postulated."}
{"id": "PMID:1080172", "title": "RNA and protein synthesis in spontaneous rosette formation by T lymphocytes.", "content": "Formation of rosettes with sheep red blood cells (SRBC) by peripheral blood lymphocytes and thymocytes has been studied. Trypsinization of these cells abolishes the capacity to form SRBC-rosettes. The process of re-establishment of this capacity to form SRBC-rosettes, in vitro, has been shown by use of metabolic inhibitors to require transcription, translation, and protein synthesis. These observations are interpreted in terms of the T cell markers as the expression of a definitive process or step in lymphoid cell differentiation.", "contents": "RNA and protein synthesis in spontaneous rosette formation by T lymphocytes. Formation of rosettes with sheep red blood cells (SRBC) by peripheral blood lymphocytes and thymocytes has been studied. Trypsinization of these cells abolishes the capacity to form SRBC-rosettes. The process of re-establishment of this capacity to form SRBC-rosettes, in vitro, has been shown by use of metabolic inhibitors to require transcription, translation, and protein synthesis. These observations are interpreted in terms of the T cell markers as the expression of a definitive process or step in lymphoid cell differentiation."}
{"id": "PMID:1080173", "title": "A simple radioimmunoassay for the enumeration of rosette-forming cells.", "content": "A simple radioimmunoassay for the enumeration of Ig bearing (Ig+) lymphocytes by rosette formation with anti-Ig, antibody coated erythrocytes (Ab-E) is described. The method is based upon labeling lymphocyte populations with 51Cr, rosetting them with Ab-E, separating the rosetted from unrosetted cells by centrifugation through a Ficoll-Hypaque layer, counting the radioactivity of the unrosetted cells, and counting the radioactivity of the unrosetted cells in a rosetting system with unreactive uncoated-E. This permits the calculation of the percentage of rosette-forming Ig+ cells. The assay eliminates the subjectivity and tediousness of counting and distinguishing between rosetted and unrosetted cells under the microscope. Being also sensitive and reliable, this radioimmunoassay may, after suitable modifications, replace the conventional counting of other types of rosettes.", "contents": "A simple radioimmunoassay for the enumeration of rosette-forming cells. A simple radioimmunoassay for the enumeration of Ig bearing (Ig+) lymphocytes by rosette formation with anti-Ig, antibody coated erythrocytes (Ab-E) is described. The method is based upon labeling lymphocyte populations with 51Cr, rosetting them with Ab-E, separating the rosetted from unrosetted cells by centrifugation through a Ficoll-Hypaque layer, counting the radioactivity of the unrosetted cells, and counting the radioactivity of the unrosetted cells in a rosetting system with unreactive uncoated-E. This permits the calculation of the percentage of rosette-forming Ig+ cells. The assay eliminates the subjectivity and tediousness of counting and distinguishing between rosetted and unrosetted cells under the microscope. Being also sensitive and reliable, this radioimmunoassay may, after suitable modifications, replace the conventional counting of other types of rosettes."}
{"id": "PMID:1080174", "title": "Visualization by immune electron microscopy of viruses associated with acute respiratory disease.", "content": "A sensitive and rapid method for detecting adenovirus and influenza viruses has been demonstrated by using both the direct and indirect immune electron microscopy technique. The indirect method was from 4 to over 32 times more sensitive than the direct method. The technique appears to have the sensitivity approaching tissue culture methods, but is much more rapid, requires less laboratory support personnel and space, and should lend itself to detection of noncytologic agents. These data suggest a new role for the electron microscope in diagnostic virology.", "contents": "Visualization by immune electron microscopy of viruses associated with acute respiratory disease. A sensitive and rapid method for detecting adenovirus and influenza viruses has been demonstrated by using both the direct and indirect immune electron microscopy technique. The indirect method was from 4 to over 32 times more sensitive than the direct method. The technique appears to have the sensitivity approaching tissue culture methods, but is much more rapid, requires less laboratory support personnel and space, and should lend itself to detection of noncytologic agents. These data suggest a new role for the electron microscope in diagnostic virology."}
{"id": "PMID:1080175", "title": "A modified assay for the detection of human adult active rosette forming lymphocytes.", "content": "A modified procedure is described for the detection of a sub-population of peripheral blood T-cells in normal human adults. This sub-population of T-cells has been called the active rosette forming cell by others. The advantages of this modified procedure compared with that previously described are: 1) the elimination of 60 mon incubation period for lymphocytes prior to the assay and 2) the elimination of the need for fetal calf serum in the assay. The mean percentage of RFC in the peripheral blood of 80 healthy adult controls using the modified procedure is 25.2+/-5.5% in buffer lacking FCS. The results were highly reproducible and comparable to the results obtained using the procedure previously described. It was concluded that a 60 min pre-incubation of lymphocytes and the use of FCS are not necessary when the appropriate SRBS: lymphocyte ratio is used in the determination of human adult active RFC. The use of this procedure in evaluating the immune competence of cancer patients is discussed.", "contents": "A modified assay for the detection of human adult active rosette forming lymphocytes. A modified procedure is described for the detection of a sub-population of peripheral blood T-cells in normal human adults. This sub-population of T-cells has been called the active rosette forming cell by others. The advantages of this modified procedure compared with that previously described are: 1) the elimination of 60 mon incubation period for lymphocytes prior to the assay and 2) the elimination of the need for fetal calf serum in the assay. The mean percentage of RFC in the peripheral blood of 80 healthy adult controls using the modified procedure is 25.2+/-5.5% in buffer lacking FCS. The results were highly reproducible and comparable to the results obtained using the procedure previously described. It was concluded that a 60 min pre-incubation of lymphocytes and the use of FCS are not necessary when the appropriate SRBS: lymphocyte ratio is used in the determination of human adult active RFC. The use of this procedure in evaluating the immune competence of cancer patients is discussed."}
{"id": "PMID:1080176", "title": "A simultaneous method for detection and quantitation of T- and B-lymphocytes.", "content": "Assay for quantitation of B- and T--lymphocyted from small volumes of heparinized man blood has been developed by simultaneously reacting Ficoll-Hypaque purified mononuclear cells with antobody-coated chicken erythrocytes to for rosettes with B-lymphocytes, and sheep erythrocytes which formed non-immune rosettes with Tlymphocytes. Distribution of various lymphocyte populations in normals are T-cells, 60.9+/-7%, B-cells 16.4+/-4%, and lymphocytes with both receptors, 1.6+/-0.6%.", "contents": "A simultaneous method for detection and quantitation of T- and B-lymphocytes. Assay for quantitation of B- and T--lymphocyted from small volumes of heparinized man blood has been developed by simultaneously reacting Ficoll-Hypaque purified mononuclear cells with antobody-coated chicken erythrocytes to for rosettes with B-lymphocytes, and sheep erythrocytes which formed non-immune rosettes with Tlymphocytes. Distribution of various lymphocyte populations in normals are T-cells, 60.9+/-7%, B-cells 16.4+/-4%, and lymphocytes with both receptors, 1.6+/-0.6%."}
{"id": "PMID:1080177", "title": "Detection of specific antigen-sensitized T-lymphocytes in human tonsils.", "content": "Tonsillar lymphocytes from children immunized against rubella were cultured with various mitogens, including phytohemagglutinin and rubella virus. Sheep red cells were subsequently added to detect rosette-forming T-lymphocytes, and the suspension tested by autoradiography to identify transformed cells. With PHA, 60--80% of the cells were transformed, of which 37% were rosette-forming T-cells. With rubella antigen only 31% of lymphocytes were transformed, but almost all of these formed rosettes. It is concluded that PHA stimulates B- as well as T-cells alone; such stimulation may therefore be a more reliable test of T-cell function and of cell-mediated immunity.", "contents": "Detection of specific antigen-sensitized T-lymphocytes in human tonsils. Tonsillar lymphocytes from children immunized against rubella were cultured with various mitogens, including phytohemagglutinin and rubella virus. Sheep red cells were subsequently added to detect rosette-forming T-lymphocytes, and the suspension tested by autoradiography to identify transformed cells. With PHA, 60--80% of the cells were transformed, of which 37% were rosette-forming T-cells. With rubella antigen only 31% of lymphocytes were transformed, but almost all of these formed rosettes. It is concluded that PHA stimulates B- as well as T-cells alone; such stimulation may therefore be a more reliable test of T-cell function and of cell-mediated immunity."}
{"id": "PMID:1080178", "title": "Effect of previous infection on antibody response of children to vaccination with capsular polysaccharide of Haemophilus influenzae Type b.", "content": "Children who had recovered from meningitis, orbital cellulitis, or epiglottitis caused by Haemophilus influenzae type b were immunized with capsular polysaccharide vaccine derived from that bacterium; some healthy siblings and adults who had not had H. influenzae infections were also vaccinated. Of 10 children who had had H. influenzae meningitis previously, only one had an antibody response to the vaccine. One child with prior H. influenzae orbital cellulitis also failed to respond. None of the children had detectable H. influenzae polysaccharide antigen in their bloodstream at the time of immunization. Two children who had had H. influenzae epiglottitis and six of seven controls without histories of H. influenzae infections responded immunologically to the vaccine. One of eight vaccinees under two years of age showed a response, and eight of 12 over two years responded well (P = 0.02). All four nonresponders over the age of two years had had H; influenzae meningitis or cellulitis. Children who had had H. influenzae meningitis responded less well to the polysaccharide vaccine than did other recipients of the vaccine; this difference could not be explained solely on the basis of age;", "contents": "Effect of previous infection on antibody response of children to vaccination with capsular polysaccharide of Haemophilus influenzae Type b. Children who had recovered from meningitis, orbital cellulitis, or epiglottitis caused by Haemophilus influenzae type b were immunized with capsular polysaccharide vaccine derived from that bacterium; some healthy siblings and adults who had not had H. influenzae infections were also vaccinated. Of 10 children who had had H. influenzae meningitis previously, only one had an antibody response to the vaccine. One child with prior H. influenzae orbital cellulitis also failed to respond. None of the children had detectable H. influenzae polysaccharide antigen in their bloodstream at the time of immunization. Two children who had had H. influenzae epiglottitis and six of seven controls without histories of H. influenzae infections responded immunologically to the vaccine. One of eight vaccinees under two years of age showed a response, and eight of 12 over two years responded well (P = 0.02). All four nonresponders over the age of two years had had H; influenzae meningitis or cellulitis. Children who had had H. influenzae meningitis responded less well to the polysaccharide vaccine than did other recipients of the vaccine; this difference could not be explained solely on the basis of age;"}
{"id": "PMID:1080179", "title": "Modification of rat uterine monoamine oxidase activity by steroid hormones.", "content": "The monoamine oxidase (MAO) activity in the ovariectomized rat uterus was significantly increased above control levels in animals given testosterone: 33% (P smaller than 0.01) with tryptamine or 34% (P smaller than 0.05) with tyramine as substrate. Activity was also higher in hydrocortisone-treated animals: 30% (P smaller than 0.05) with tyramine or 25% (P smaller than 0.05) with tryptamine as substrate. Progesterone injection increased MAO activity toward tyramine by 20% but towards tryptamine by only 8%. The differences are not statistically significant but are believed to be real since they were reproducible. MAO activity in oestradiol-treated animals was 11% less than in the controls for both substrates. Although they are not significant differences, they were reproducible. No change in MAO activity was observed in the cerebellum, hypothalamus or anterior pituitary of ovariectomized rats after steroid treatment. At oestrus the enzyme activity in the uterus was lower than at dioestrus or prooestrus when beta-phenylethylamine was the substrate. When 5-hydroxytryptamine was used to measure enzyme activity, the same values were found at oestrus, dioestrus and prooestrus. Ovariectomy did not cause any changes in MAO activity in any of the tissues. Effects of the steroids in vivo were probably not due to a direct action on the enzyme since only at high concentration did they have any effect on the mitochondrial enzyme activity of the uterus in vivo.", "contents": "Modification of rat uterine monoamine oxidase activity by steroid hormones. The monoamine oxidase (MAO) activity in the ovariectomized rat uterus was significantly increased above control levels in animals given testosterone: 33% (P smaller than 0.01) with tryptamine or 34% (P smaller than 0.05) with tyramine as substrate. Activity was also higher in hydrocortisone-treated animals: 30% (P smaller than 0.05) with tyramine or 25% (P smaller than 0.05) with tryptamine as substrate. Progesterone injection increased MAO activity toward tyramine by 20% but towards tryptamine by only 8%. The differences are not statistically significant but are believed to be real since they were reproducible. MAO activity in oestradiol-treated animals was 11% less than in the controls for both substrates. Although they are not significant differences, they were reproducible. No change in MAO activity was observed in the cerebellum, hypothalamus or anterior pituitary of ovariectomized rats after steroid treatment. At oestrus the enzyme activity in the uterus was lower than at dioestrus or prooestrus when beta-phenylethylamine was the substrate. When 5-hydroxytryptamine was used to measure enzyme activity, the same values were found at oestrus, dioestrus and prooestrus. Ovariectomy did not cause any changes in MAO activity in any of the tissues. Effects of the steroids in vivo were probably not due to a direct action on the enzyme since only at high concentration did they have any effect on the mitochondrial enzyme activity of the uterus in vivo."}
{"id": "PMID:1080181", "title": "Genetic control of immune responses in vitro. VI. Experimental conditions for the development of helper T-cell activity specific for the terpolymer L-glutamic aicd60-L-alanine30-L-tyrosine10 (GAT) in nonresponder mice.", "content": "Mice which are genetic nonresponders to the random terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) not only fail to develop GAT-specific antibody responses when stimulated with soluble GAT either in vivo or in vitro, but develop GAT-specific T cells which suppress the GAT-specific plaque-forming cell response of normal nonresponder mice stimulated with GAT complexed to methylated bovine serum albumin (MBSA).Thus, both responder and nonresponder mice have T cells which recognize GAT. However, nonresponder mice can develop GAT-specific helper T cells if immunized with GAT bound to MBSA or to macrophages. The relevance of Ir gene-controlled responses is discussed.", "contents": "Genetic control of immune responses in vitro. VI. Experimental conditions for the development of helper T-cell activity specific for the terpolymer L-glutamic aicd60-L-alanine30-L-tyrosine10 (GAT) in nonresponder mice. Mice which are genetic nonresponders to the random terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) not only fail to develop GAT-specific antibody responses when stimulated with soluble GAT either in vivo or in vitro, but develop GAT-specific T cells which suppress the GAT-specific plaque-forming cell response of normal nonresponder mice stimulated with GAT complexed to methylated bovine serum albumin (MBSA).Thus, both responder and nonresponder mice have T cells which recognize GAT. However, nonresponder mice can develop GAT-specific helper T cells if immunized with GAT bound to MBSA or to macrophages. The relevance of Ir gene-controlled responses is discussed."}
{"id": "PMID:1080182", "title": "Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera.", "content": "Human sera were tested for cytotoxicity to pairs of long-term tissue-cultured cell lines. Each pair had been derived from the same individual and one of the pairs possessed the characteristics of either \"T\" or \"B\" cells. The alloantisera used were HL-A-typing reagents or sera obtained from Amish multiparas. Selected cytotoxicity was found against the  B-cell lines by direct testing. Cytotoxicity was abolished by absorption with B-cell line but not by absorption with the T-cell lines. The results suggest that a group of allotypic antigens may be expressed exculsively on human B cells.", "contents": "Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera. Human sera were tested for cytotoxicity to pairs of long-term tissue-cultured cell lines. Each pair had been derived from the same individual and one of the pairs possessed the characteristics of either \"T\" or \"B\" cells. The alloantisera used were HL-A-typing reagents or sera obtained from Amish multiparas. Selected cytotoxicity was found against the  B-cell lines by direct testing. Cytotoxicity was abolished by absorption with B-cell line but not by absorption with the T-cell lines. The results suggest that a group of allotypic antigens may be expressed exculsively on human B cells."}
{"id": "PMID:1080183", "title": "Non-physician development of problem lists from office records.", "content": "It has been argued that conversion of office records to the problem-oriented medical record entails a prohibitive cost in money and physician time. This study reports an evaluation of the cost of preparing problem lists from existing ambulatory patient records by non-physician personnel. The problem lists constructed by non-physician personnel were judged by physicians to be accurate, and the cost was much less than that required when physicians reviewed charts and prepared problem lists themselves. The method described here is an accurate and cost-effective way to begin conversion of outpatient records to P.O.M.R.", "contents": "Non-physician development of problem lists from office records. It has been argued that conversion of office records to the problem-oriented medical record entails a prohibitive cost in money and physician time. This study reports an evaluation of the cost of preparing problem lists from existing ambulatory patient records by non-physician personnel. The problem lists constructed by non-physician personnel were judged by physicians to be accurate, and the cost was much less than that required when physicians reviewed charts and prepared problem lists themselves. The method described here is an accurate and cost-effective way to begin conversion of outpatient records to P.O.M.R."}
{"id": "PMID:1080184", "title": "Effects of sudden changes in external sodium concentration on twitch tension in isolated muscle fibers.", "content": "When [Na] was suddenly introduced to single muscle fibers (Xenopus or frog), which had been pretreated with Na-free solution (Tris-substituted), the time-course of twitch recovery was very variable, half-time ranging from less than 1 S to 5 S. The [Na] vs. twitch height relationship was also variable. In small Xenopus fibers, decreases of [Na] to 50% increased the twitch, while in large Xenopus fibers twitch height remained constant or decreased as [Na] was decreased to 50%. The apparent diffusion constant (D') of Na+ or K+, calculated from the time-course of twitch recovery and the [Na] vs. twitch relation, and from the time-course of the slow repolarization upon sudden reduction of [K] was about 1-1.5 X 10(-6) cm2/S. This is one order of magnitude smaller than the diffusion constants in an aqueous solution. Even if the tortuosity factor of the T system is taken into account, there remains a substantial discrepancy. Although our value of D' is subject to various errors, if we accept the value, the twitch recovery is predicted to be either very quick or slow depending upon the variation of [Na]-twitch relation and fiber size. Thus, both quick and slow twitch recoveries can be explained by the diffusion time of Na+ in the T system, and therefore the results are consistent with the idea that the T system is excitable.", "contents": "Effects of sudden changes in external sodium concentration on twitch tension in isolated muscle fibers. When [Na] was suddenly introduced to single muscle fibers (Xenopus or frog), which had been pretreated with Na-free solution (Tris-substituted), the time-course of twitch recovery was very variable, half-time ranging from less than 1 S to 5 S. The [Na] vs. twitch height relationship was also variable. In small Xenopus fibers, decreases of [Na] to 50% increased the twitch, while in large Xenopus fibers twitch height remained constant or decreased as [Na] was decreased to 50%. The apparent diffusion constant (D') of Na+ or K+, calculated from the time-course of twitch recovery and the [Na] vs. twitch relation, and from the time-course of the slow repolarization upon sudden reduction of [K] was about 1-1.5 X 10(-6) cm2/S. This is one order of magnitude smaller than the diffusion constants in an aqueous solution. Even if the tortuosity factor of the T system is taken into account, there remains a substantial discrepancy. Although our value of D' is subject to various errors, if we accept the value, the twitch recovery is predicted to be either very quick or slow depending upon the variation of [Na]-twitch relation and fiber size. Thus, both quick and slow twitch recoveries can be explained by the diffusion time of Na+ in the T system, and therefore the results are consistent with the idea that the T system is excitable."}
{"id": "PMID:1080185", "title": "Psychiatric complications following coronary bypass surgery.", "content": "Previous studies of psychiatric complications following open heart surgery have included few if any patients who had coronary bypass surgery. This experiment reports the relative incidence of psychiatric complications in a sample of 97 open heart surgery patients of whom 51 patients (53 per cent) had coronary bypass surgery. The results suggest that the incidence of psychiatric symptoms following coronary bypass surgery is significantly lower (16 per cent) than that following cardiac valvular surgery (41 per cent). Several possible reasons for this large discrepancy in incidence of psychiatric complications are considered.", "contents": "Psychiatric complications following coronary bypass surgery. Previous studies of psychiatric complications following open heart surgery have included few if any patients who had coronary bypass surgery. This experiment reports the relative incidence of psychiatric complications in a sample of 97 open heart surgery patients of whom 51 patients (53 per cent) had coronary bypass surgery. The results suggest that the incidence of psychiatric symptoms following coronary bypass surgery is significantly lower (16 per cent) than that following cardiac valvular surgery (41 per cent). Several possible reasons for this large discrepancy in incidence of psychiatric complications are considered."}
{"id": "PMID:1080186", "title": "Acute reduction of brain serotonin and 5-HIAA following food consumption: correlation with the ratio of serum tryptophan to the sum of competing amino acids.", "content": "In a series of experiments designed to test further our hypothesis that brain tryptophan and 5-hydroxyindole levels vary post-prandially as a function of the serum tryptophan to competitor ratio (i.e., ratio of serum tryptophan concentration to the sum of the concentrations of competing neutral amino acids), rats fasted overnight were allosed to consume for two hours single meals containing carbohydrates, fats, and various amino acids: Diet 1 contained no amino acids; Diet 2 contained five neutral amino acids known to compete with tryptophan for uptake into the brain; Diet 3 lacked tryptophan and the five competitors, but contained twelve non-neutral amino acids found in dietary protein; Diet 4 contained tryptophan and these same twelve non-neutral amino acids. The diet-induced modifications in brain tryptophan and 5-hydroxyindoles followed closely the changes in the serum tryptophan/competitor ratio, but not in serum tryptophan alone: The ingestion of Diet 4 raised serum tryptophan, but not the serum ratio, brain tryptophan or 5-hydroxyindoles; Diet 3 did not raise serum tryptophan, but did elevate the serum ratio, brain tryptophan and 5-hydroxyindoles. Diet 2 caused major reductions in serum tryptophan, the serum ratio, and brain tryptophan and the 5-hydroxyindoles. This diet might provide a simple, attraumatic means for lowering brain serotonin in studies of the physiology or serotonergin neurons. These studies provide further evidence that the effect of insulin on brain tryptophan uptake is not direct, but instead mediated via insulin-induced changes in the serum tryptophan/competitor ratio.", "contents": "Acute reduction of brain serotonin and 5-HIAA following food consumption: correlation with the ratio of serum tryptophan to the sum of competing amino acids. In a series of experiments designed to test further our hypothesis that brain tryptophan and 5-hydroxyindole levels vary post-prandially as a function of the serum tryptophan to competitor ratio (i.e., ratio of serum tryptophan concentration to the sum of the concentrations of competing neutral amino acids), rats fasted overnight were allosed to consume for two hours single meals containing carbohydrates, fats, and various amino acids: Diet 1 contained no amino acids; Diet 2 contained five neutral amino acids known to compete with tryptophan for uptake into the brain; Diet 3 lacked tryptophan and the five competitors, but contained twelve non-neutral amino acids found in dietary protein; Diet 4 contained tryptophan and these same twelve non-neutral amino acids. The diet-induced modifications in brain tryptophan and 5-hydroxyindoles followed closely the changes in the serum tryptophan/competitor ratio, but not in serum tryptophan alone: The ingestion of Diet 4 raised serum tryptophan, but not the serum ratio, brain tryptophan or 5-hydroxyindoles; Diet 3 did not raise serum tryptophan, but did elevate the serum ratio, brain tryptophan and 5-hydroxyindoles. Diet 2 caused major reductions in serum tryptophan, the serum ratio, and brain tryptophan and the 5-hydroxyindoles. This diet might provide a simple, attraumatic means for lowering brain serotonin in studies of the physiology or serotonergin neurons. These studies provide further evidence that the effect of insulin on brain tryptophan uptake is not direct, but instead mediated via insulin-induced changes in the serum tryptophan/competitor ratio."}
{"id": "PMID:1080188", "title": "Babinski response: stimulus and effector.", "content": "This is an electromyographic study of the activity in flexor and extensor muscles of the big toe in 22 patients with a Babinski sign and 49 controls, after mechanical or electrical stimulation of the sole. The results indicate: (1) the Babinski sign is mediated by the extensor hallucis longus (EHL), and not by the extensor hallucis brevis; (2) electrical stimuli may fail to activate the EHL in these patients, and conversely may evoke EHL reflexes in control subjects; (3) in skin reflexes, electrical and mechanical stimuli are not freely interchangeable.", "contents": "Babinski response: stimulus and effector. This is an electromyographic study of the activity in flexor and extensor muscles of the big toe in 22 patients with a Babinski sign and 49 controls, after mechanical or electrical stimulation of the sole. The results indicate: (1) the Babinski sign is mediated by the extensor hallucis longus (EHL), and not by the extensor hallucis brevis; (2) electrical stimuli may fail to activate the EHL in these patients, and conversely may evoke EHL reflexes in control subjects; (3) in skin reflexes, electrical and mechanical stimuli are not freely interchangeable."}
{"id": "PMID:1080189", "title": "Radiological abnormalities in temporal lobe epilepsy with clinicopathological correlations.", "content": "In 73 patients with drug-resistant temporal lobe epilepsy submitted to an unilateral anterior temporal lobectomy the radiographs were studied to see if there were any correlation with the pathology subsequently found and with the outcome of the operation. A small middle cranial fossa, focal calcification, and temporal horn displacement are often better indices of the underlying pathology than temporal horn dilatation alone. In a small number of cases, however, radiological changes were seen on the side opposite to an unilateral EEG focus, thus suggesting bilateral disease.", "contents": "Radiological abnormalities in temporal lobe epilepsy with clinicopathological correlations. In 73 patients with drug-resistant temporal lobe epilepsy submitted to an unilateral anterior temporal lobectomy the radiographs were studied to see if there were any correlation with the pathology subsequently found and with the outcome of the operation. A small middle cranial fossa, focal calcification, and temporal horn displacement are often better indices of the underlying pathology than temporal horn dilatation alone. In a small number of cases, however, radiological changes were seen on the side opposite to an unilateral EEG focus, thus suggesting bilateral disease."}
{"id": "PMID:1080190", "title": "Hemianopic colour blindness.", "content": "A man developed cortical blindness after cerebral infarction in the distribution of both posterior cerebral arteries. When he recovered from this condition, he was found to be colour blind in the left visual field, but not in the right. This unusual situation resulted in apparently contradictory performances on hemifield and free-field tasks of colour discrimination, naming, and recognition. The contradictions may be explained by interhemispheric competition between a hemisphere which could discriminate colours and a hemisphere which was colour blind.", "contents": "Hemianopic colour blindness. A man developed cortical blindness after cerebral infarction in the distribution of both posterior cerebral arteries. When he recovered from this condition, he was found to be colour blind in the left visual field, but not in the right. This unusual situation resulted in apparently contradictory performances on hemifield and free-field tasks of colour discrimination, naming, and recognition. The contradictions may be explained by interhemispheric competition between a hemisphere which could discriminate colours and a hemisphere which was colour blind."}
{"id": "PMID:1080191", "title": "Intracranial hydatid cysts. Study of 17 cases.", "content": "The authors report 17 cases of intracranial hydatid cyst out of 500 brain tumors seen in 20 years, and analyze clinical, investigative, diagnostic, and operative findings.", "contents": "Intracranial hydatid cysts. Study of 17 cases. The authors report 17 cases of intracranial hydatid cyst out of 500 brain tumors seen in 20 years, and analyze clinical, investigative, diagnostic, and operative findings."}
{"id": "PMID:1080192", "title": "Portsmouth syndrome: review of the literature and clinicopathological correlation.", "content": "Hereditary and acquired abnormalities of platelet function are recognized more frequently.  Recent advances in platelet physiology have led to new tests of platelet function.  Assessment of platelet adhesiveness and aggregation have allowed more definitive classification of platelet dysfunction.  Platelet abnormalities will not be diagnosed before surgery.  It would be impractical to perform these tests for platelet function as screening procedures on all patients; however, they should be considered as an important part of the laboratory screening for abnormal postoperative bleeding.", "contents": "Portsmouth syndrome: review of the literature and clinicopathological correlation. Hereditary and acquired abnormalities of platelet function are recognized more frequently.  Recent advances in platelet physiology have led to new tests of platelet function.  Assessment of platelet adhesiveness and aggregation have allowed more definitive classification of platelet dysfunction.  Platelet abnormalities will not be diagnosed before surgery.  It would be impractical to perform these tests for platelet function as screening procedures on all patients; however, they should be considered as an important part of the laboratory screening for abnormal postoperative bleeding."}
{"id": "PMID:1080193", "title": "Comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. I. Non-specific inflammation due to the intradermal injection of turpentine.", "content": "Guinea-pigs were treated with agents which lowered the serum complement levels and the effect on the inflammatory reaction to intradermal turpentine was studied. A comparison was made between two antisera to beta1C/beta1A globulin which had been prepared in different ways. One was made using C3 fixed to zymosan particles as the antigen, and the other using chemically extracted beta 1C/beta1A globulin. The antisera to beta1C/beta1A globulin (zymosan) was found to have the greater anti-inflammatory activity. The effect of each on the parameters of complement studied, and on the circulating platelet count, was much the same. An antiserum to serum factors fixed to zymosan during C3 activication was prepared free of anti-beta1C/beta1A globulin antibodies. This was found to have some of the anti-inflammatory activity of the anti-beta1C/beta1A globulin (zymosan), as well as lowering total complement levels. Anti-gamma globulin andheterologous immune complexes also lowered serum complement levels and were antiinflammatory. However, all the above agents were also found to reduce the number of circulating platelets. This was in contrast to CVF which reduced complement levels by 90 per cent. but had no effect on platelet numbers. CVF also reduced the inflammatory response but was no more effective than the other agents. In view of these findings a role for the participation of the complement system, possibly involving the alternate pathway of activation of C3, is discussed as well as the participation of platelets in the reaction.", "contents": "Comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. I. Non-specific inflammation due to the intradermal injection of turpentine. Guinea-pigs were treated with agents which lowered the serum complement levels and the effect on the inflammatory reaction to intradermal turpentine was studied. A comparison was made between two antisera to beta1C/beta1A globulin which had been prepared in different ways. One was made using C3 fixed to zymosan particles as the antigen, and the other using chemically extracted beta 1C/beta1A globulin. The antisera to beta1C/beta1A globulin (zymosan) was found to have the greater anti-inflammatory activity. The effect of each on the parameters of complement studied, and on the circulating platelet count, was much the same. An antiserum to serum factors fixed to zymosan during C3 activication was prepared free of anti-beta1C/beta1A globulin antibodies. This was found to have some of the anti-inflammatory activity of the anti-beta1C/beta1A globulin (zymosan), as well as lowering total complement levels. Anti-gamma globulin andheterologous immune complexes also lowered serum complement levels and were antiinflammatory. However, all the above agents were also found to reduce the number of circulating platelets. This was in contrast to CVF which reduced complement levels by 90 per cent. but had no effect on platelet numbers. CVF also reduced the inflammatory response but was no more effective than the other agents. In view of these findings a role for the participation of the complement system, possibly involving the alternate pathway of activation of C3, is discussed as well as the participation of platelets in the reaction."}
{"id": "PMID:1080196", "title": "The influence of dental plaques on the microvasculature of the oral mucous membrane.", "content": "This investigation was designed to explore the effect of plaque, obtained at different stages of accumulation, on the microvasculature of the hamster cheek pouch. Five groups of three dental students followed an oral hygiene abstinence program for 22 days. At day 2, 9, and 22, plaque was sampled from different anterior teeth, pooled and suspended in a reduced diluting fluid. Each of the three pooled plaque suspensions was then introduced into the right or left cheek pouche of five hamsters. Microvascular changes in the pouches were then observed and recorded photomicrographically until vascular reconstitution occurred. Plaque and gingival indices were taken, and the microbial composition of the plaque was determined according to gram-staining characteristics and morphology. The results indicate that the microvascular response was not a function of age and composition of plaque. This finding is based on the observation that 2-, 9-, and 22-day-old plaque induced similar microvascular damage which required approximately the same recovery period of 9 days to reconsitute normal microvascular function.", "contents": "The influence of dental plaques on the microvasculature of the oral mucous membrane. This investigation was designed to explore the effect of plaque, obtained at different stages of accumulation, on the microvasculature of the hamster cheek pouch. Five groups of three dental students followed an oral hygiene abstinence program for 22 days. At day 2, 9, and 22, plaque was sampled from different anterior teeth, pooled and suspended in a reduced diluting fluid. Each of the three pooled plaque suspensions was then introduced into the right or left cheek pouche of five hamsters. Microvascular changes in the pouches were then observed and recorded photomicrographically until vascular reconstitution occurred. Plaque and gingival indices were taken, and the microbial composition of the plaque was determined according to gram-staining characteristics and morphology. The results indicate that the microvascular response was not a function of age and composition of plaque. This finding is based on the observation that 2-, 9-, and 22-day-old plaque induced similar microvascular damage which required approximately the same recovery period of 9 days to reconsitute normal microvascular function."}
{"id": "PMID:1080197", "title": "Microdetermination of ergocalciferol (vitamin D2) in pharmaceutical preparations by differential spectroscopy using trifluoroacetic acid in the absence of vitamin A.", "content": "A differential spectroscopy method for the determination of microgram quantities of ergocalciferol, using trifluoroacetic acid and hydrogen peroxide, was applied to commercially available pharmaceutical preparations. Tablets were powdered and extracted with methanol after being made basic with diethylamine. The methanol was concentrated, chloroform was added, and the mixture was chromatographed on neutral alumina using chloroform as the eluent. Ergocalciferol was determined in an aliquot of the eluate by differential spectroscopy and was compared with a reference ergocalciferol standard treated similarly. The method applied to low potency formulations (2.5-6 mug/tablet) is simple and quantitative, needs less than 5 hr for completion, and has a precision of less than +/- 2%. A simple and accurate chromatographic method for the determination of ergocalciferol in stabilized powders and high potency tablets, which precludes the necessity of solvent-solvent extraction, was also developed. The results are comparable with those obtained by the rat bioassay and the USP XVIII chemical assay.", "contents": "Microdetermination of ergocalciferol (vitamin D2) in pharmaceutical preparations by differential spectroscopy using trifluoroacetic acid in the absence of vitamin A. A differential spectroscopy method for the determination of microgram quantities of ergocalciferol, using trifluoroacetic acid and hydrogen peroxide, was applied to commercially available pharmaceutical preparations. Tablets were powdered and extracted with methanol after being made basic with diethylamine. The methanol was concentrated, chloroform was added, and the mixture was chromatographed on neutral alumina using chloroform as the eluent. Ergocalciferol was determined in an aliquot of the eluate by differential spectroscopy and was compared with a reference ergocalciferol standard treated similarly. The method applied to low potency formulations (2.5-6 mug/tablet) is simple and quantitative, needs less than 5 hr for completion, and has a precision of less than +/- 2%. A simple and accurate chromatographic method for the determination of ergocalciferol in stabilized powders and high potency tablets, which precludes the necessity of solvent-solvent extraction, was also developed. The results are comparable with those obtained by the rat bioassay and the USP XVIII chemical assay."}
{"id": "PMID:1080198", "title": "Tetrodotoxin binding to normal depolarized frog muscle and the conductance of a single sodium channel.", "content": "1. We have examined the binding of tritium-labelled and unlabelled tetrodotoxin to frog twitch muscle. Bio-assay as well as radioisotope experiments show a saturable component of tetrodotoxin binding with a binding capacity of about 22 p-mole/g wet wt., and a dissociation constant of about 5 nM. 2. If the observed uptake of tetrodotoxin by muscles represents one-to-one binding of the drug to sodium channels, the channel density is about 380 channels/mum2 of a muscle fibre's surface membrane. On the basis of this result and electrical measurements of sodium conductance in frog muscle, we calculate that the conductance of a single sodium channel is of the order of 10(-12) reciprocal ohms. This is one to two orders of magnitude less than previous estimates. 3. We have looked for an effect of membrane depolarization on saturable tetrodotoxin binding, and have found none. This suggests that there is little molecular interaction between the \"gating\" portion of the sodium channel molecule, and that which binds tetrodotoxin.", "contents": "Tetrodotoxin binding to normal depolarized frog muscle and the conductance of a single sodium channel. 1. We have examined the binding of tritium-labelled and unlabelled tetrodotoxin to frog twitch muscle. Bio-assay as well as radioisotope experiments show a saturable component of tetrodotoxin binding with a binding capacity of about 22 p-mole/g wet wt., and a dissociation constant of about 5 nM. 2. If the observed uptake of tetrodotoxin by muscles represents one-to-one binding of the drug to sodium channels, the channel density is about 380 channels/mum2 of a muscle fibre's surface membrane. On the basis of this result and electrical measurements of sodium conductance in frog muscle, we calculate that the conductance of a single sodium channel is of the order of 10(-12) reciprocal ohms. This is one to two orders of magnitude less than previous estimates. 3. We have looked for an effect of membrane depolarization on saturable tetrodotoxin binding, and have found none. This suggests that there is little molecular interaction between the \"gating\" portion of the sodium channel molecule, and that which binds tetrodotoxin."}
{"id": "PMID:1080200", "title": "Patterns of antinuclear antibodies and lupus-activating drugs.", "content": "Patterns of antinuclear antibodies (ANA) occurring in individuals consuming lupus-activating drugs who have not developed systemic lupus erythematosus (SLE), may give insight into their mode of action as SLE triggering agents. We have studied the antigenic specificity of ANA induced by isoniazid (INH), anticonvulsants and chlorpromazine. ANA found in INH treated subjects are primarily directed at soluble nucleoprotein (sNP), an antigen which is physicochemically altered in vivo by INH. Antibodies to INH altered sNP were found in 78 per cent of 214 subjects receiving INH, while none had anti-DNA antibodies. Different anticonvulsants give rise to different patterns of ANA. Antibodies to Sm antigen were found only in patients receiving hydantoins. Antibodies to native and denatured DNA were found in 14 per cent and 22 per cent respectively, of 170 patients receiving anticonvulsants. Antibodies to denatured DNA were most frequently found in patients receiving chlorpromazine, which correlated with the known reactivity of this drug to denatured DNA.", "contents": "Patterns of antinuclear antibodies and lupus-activating drugs. Patterns of antinuclear antibodies (ANA) occurring in individuals consuming lupus-activating drugs who have not developed systemic lupus erythematosus (SLE), may give insight into their mode of action as SLE triggering agents. We have studied the antigenic specificity of ANA induced by isoniazid (INH), anticonvulsants and chlorpromazine. ANA found in INH treated subjects are primarily directed at soluble nucleoprotein (sNP), an antigen which is physicochemically altered in vivo by INH. Antibodies to INH altered sNP were found in 78 per cent of 214 subjects receiving INH, while none had anti-DNA antibodies. Different anticonvulsants give rise to different patterns of ANA. Antibodies to Sm antigen were found only in patients receiving hydantoins. Antibodies to native and denatured DNA were found in 14 per cent and 22 per cent respectively, of 170 patients receiving anticonvulsants. Antibodies to denatured DNA were most frequently found in patients receiving chlorpromazine, which correlated with the known reactivity of this drug to denatured DNA."}
{"id": "PMID:1080201", "title": "2-Halo derivatives of 3'-acetamido-3'-deoxyadenosine.", "content": "9-(3-Acetamido-2,5-di-O-acetyl-3-deoxy-beta-D-ribofuranosyl)-2,6-dichloropurine (VI) prepared by the mercuric cyanide catalyzed reaction of 3-acetamido-2,5-di-O-acetyl-3-deoxy-D-ribofuranosyl chloride (III) with 2,6-dichloropurine, was converted by standard reactions to 3'-acetamido-2-chloro-3'-deoxyadenosine (VII) and 3'-acetamido-3'-deoxy-2-fluoroadenosine (X). The 2-chloroadenine nucleoside VII was not cytotoxic, but the 2-fluoroadenine nucleoside X was moderately so, and its cytotoxicity to a subline of H.Ep.-2 cells resistant to 2-fluoroadenine indicates that its activity is due to the intact nucleoside.", "contents": "2-Halo derivatives of 3'-acetamido-3'-deoxyadenosine. 9-(3-Acetamido-2,5-di-O-acetyl-3-deoxy-beta-D-ribofuranosyl)-2,6-dichloropurine (VI) prepared by the mercuric cyanide catalyzed reaction of 3-acetamido-2,5-di-O-acetyl-3-deoxy-D-ribofuranosyl chloride (III) with 2,6-dichloropurine, was converted by standard reactions to 3'-acetamido-2-chloro-3'-deoxyadenosine (VII) and 3'-acetamido-3'-deoxy-2-fluoroadenosine (X). The 2-chloroadenine nucleoside VII was not cytotoxic, but the 2-fluoroadenine nucleoside X was moderately so, and its cytotoxicity to a subline of H.Ep.-2 cells resistant to 2-fluoroadenine indicates that its activity is due to the intact nucleoside."}
{"id": "PMID:1080202", "title": "Synthesis and antiinflammatory activity of some 1,6-methano[10]annuleneacetic acids.", "content": "Some new approaches to the 1,6-methano[10]annulene system are described. The routes were used to prepare 1,6-methano[10]annulene-3-acetic acid and the alpha-methyl analog. The compounds showed antinflammatory and analgesic activity, though less than that of corresponding naphthalene compounds; the possible effect of the chirality of the annulene on the observed biological activity is discussed.", "contents": "Synthesis and antiinflammatory activity of some 1,6-methano[10]annuleneacetic acids. Some new approaches to the 1,6-methano[10]annulene system are described. The routes were used to prepare 1,6-methano[10]annulene-3-acetic acid and the alpha-methyl analog. The compounds showed antinflammatory and analgesic activity, though less than that of corresponding naphthalene compounds; the possible effect of the chirality of the annulene on the observed biological activity is discussed."}
{"id": "PMID:1080207", "title": "Cytophysiological basis of disruptive pigmentary patterns in the leopard frog Rana pipiens. II. Wild type and mutant cell-specific patterns.", "content": "A new pattern index, Ip, is introduced and used to compare patterns of wild type, burnsi, and kandiyohi chromatophores in the leopard frog, Rana pipiens. Wild type chromatophores are hyperdispersed over distances within cellular contact, and it is concluded that this hyperdispersion results from contact-mediated negative interactions. The hyperdispersion is less strong in spot cells than interspot, and extends over larger areas in burnsi than in wild type epidermis. Over areas greater than chromatophore size, patterns are either random or clumped. Patterning of kandiyohi melanophores is clumped into aggregates small enough to be within the range of cellular contact, suggesting a lack of contact inhibition among these cells. The possible roles of cellular properties and the extracellular environment in pattern determination are discussed.", "contents": "Cytophysiological basis of disruptive pigmentary patterns in the leopard frog Rana pipiens. II. Wild type and mutant cell-specific patterns. A new pattern index, Ip, is introduced and used to compare patterns of wild type, burnsi, and kandiyohi chromatophores in the leopard frog, Rana pipiens. Wild type chromatophores are hyperdispersed over distances within cellular contact, and it is concluded that this hyperdispersion results from contact-mediated negative interactions. The hyperdispersion is less strong in spot cells than interspot, and extends over larger areas in burnsi than in wild type epidermis. Over areas greater than chromatophore size, patterns are either random or clumped. Patterning of kandiyohi melanophores is clumped into aggregates small enough to be within the range of cellular contact, suggesting a lack of contact inhibition among these cells. The possible roles of cellular properties and the extracellular environment in pattern determination are discussed."}
{"id": "PMID:1080208", "title": "Evidence for diaphragms in the pores of annulate lamellae and the nuclear membrane.", "content": "Interpretation of cross and tangential sections of the annulate lamellae and nuclear membrane of Rana pipiens oocytes provides evidence in these structures for the existence of disphragms spanning the pores. The evidence appears to rule out explanations ascribing such diaphragms to an optical artifact. More detailed description is given of a component of the pore complex only briefly described heretofore and now called the \"intracisternal ring.\" The varied results and interpretations of studies of the pore complex in various cells are discussed.", "contents": "Evidence for diaphragms in the pores of annulate lamellae and the nuclear membrane. Interpretation of cross and tangential sections of the annulate lamellae and nuclear membrane of Rana pipiens oocytes provides evidence in these structures for the existence of disphragms spanning the pores. The evidence appears to rule out explanations ascribing such diaphragms to an optical artifact. More detailed description is given of a component of the pore complex only briefly described heretofore and now called the \"intracisternal ring.\" The varied results and interpretations of studies of the pore complex in various cells are discussed."}
{"id": "PMID:1080210", "title": "Massive postpartum right renal hemorrhage.", "content": "All reported cases of massive postpartum right renal hemorrhage have involved healthy young primigravidas and blacks have predominated (4 of 7 women). Coagulopathies and underlying renal disease have been absent. Hematuria was painless in 5 of 8 cases. Hemorrhage began within 24 hours in 1 case, within 48 hours in 4 cases and 4 days post partum in 3 cases. Our first case is the only report in which hemorrhage has occurred in a primipara. Failure of closure or reopening of pyelovenous channels is suggested as the pathogenesis. The hemorrhage has been self-limiting, requiring no more than 1,500 cc whole blood replacement. Bleeding should stop spontaneously, and rapid renal pelvic clot lysis should follow with maintenance of adequate urine output and Foley catheter bladder decompression. To date surgical intervention has not been necessary.", "contents": "Massive postpartum right renal hemorrhage. All reported cases of massive postpartum right renal hemorrhage have involved healthy young primigravidas and blacks have predominated (4 of 7 women). Coagulopathies and underlying renal disease have been absent. Hematuria was painless in 5 of 8 cases. Hemorrhage began within 24 hours in 1 case, within 48 hours in 4 cases and 4 days post partum in 3 cases. Our first case is the only report in which hemorrhage has occurred in a primipara. Failure of closure or reopening of pyelovenous channels is suggested as the pathogenesis. The hemorrhage has been self-limiting, requiring no more than 1,500 cc whole blood replacement. Bleeding should stop spontaneously, and rapid renal pelvic clot lysis should follow with maintenance of adequate urine output and Foley catheter bladder decompression. To date surgical intervention has not been necessary."}
{"id": "PMID:1080211", "title": "Colonic neoplasms following ureterosigmoidostomy.", "content": "Attention is drawn to the occurrence of colonic tumors at the site of ureterosigmoidostomy. A case of carcinoma is described, the literature is reviewed and regular long-term followup is emphasized.", "contents": "Colonic neoplasms following ureterosigmoidostomy. Attention is drawn to the occurrence of colonic tumors at the site of ureterosigmoidostomy. A case of carcinoma is described, the literature is reviewed and regular long-term followup is emphasized."}
{"id": "PMID:1080214", "title": "Clinical studies on ampicillin-cloxacillin for intravenous injection (Rectocillin 'Kyowa') (author's transl).", "content": "(1) Ampicillin-cloxacillin for intravenous injection (Rectocillin 'Kyowa') was administered to 9 patients 1 g or 2 g daily for protection of postoperative infections, and good result was obtained in all cases. Eight out of 9 cases were with renal insufficiency. No side effect was encountered. (2) Rectocillin was administered to 14 patients with infections. The treatment was remarkably effective in 4 cases, and effective in 6 cases. In the cases with renal insufficiency, a daily dose of 1 g was effective. The dose administered to the patients with normal renal function was 2 g or 4 g daily. Neither clinical nor laboratory findings showed evidence of side effect.", "contents": "Clinical studies on ampicillin-cloxacillin for intravenous injection (Rectocillin 'Kyowa') (author's transl). (1) Ampicillin-cloxacillin for intravenous injection (Rectocillin 'Kyowa') was administered to 9 patients 1 g or 2 g daily for protection of postoperative infections, and good result was obtained in all cases. Eight out of 9 cases were with renal insufficiency. No side effect was encountered. (2) Rectocillin was administered to 14 patients with infections. The treatment was remarkably effective in 4 cases, and effective in 6 cases. In the cases with renal insufficiency, a daily dose of 1 g was effective. The dose administered to the patients with normal renal function was 2 g or 4 g daily. Neither clinical nor laboratory findings showed evidence of side effect."}
{"id": "PMID:1080215", "title": "Rod late receptor potential and rhodopsin concentration of an isolated frog retina.", "content": "The relation of the threshold of the late receptor potential (late RP) and ERG b-wave with the rhodopsin concentration in the rod outer segments of an isolated bullfrog retina were studied by simultaneous recordings of electrical responses and spectral change. The curve indicating log threshold rise of the late RP with the decrease of rhodopsin showed two phases separated by a kink, which was found at 50% rhodopsin concentration; the b-wave threshold, however, was linearly related to rhodopsin concentration. From the intensity response curves (IR curves) at various rhodopsin concentrations, the amplitude decrease of both waves following bleaching light adaptation was compared and the mode of the conversion from the late RP to the b-wave was discussed.", "contents": "Rod late receptor potential and rhodopsin concentration of an isolated frog retina. The relation of the threshold of the late receptor potential (late RP) and ERG b-wave with the rhodopsin concentration in the rod outer segments of an isolated bullfrog retina were studied by simultaneous recordings of electrical responses and spectral change. The curve indicating log threshold rise of the late RP with the decrease of rhodopsin showed two phases separated by a kink, which was found at 50% rhodopsin concentration; the b-wave threshold, however, was linearly related to rhodopsin concentration. From the intensity response curves (IR curves) at various rhodopsin concentrations, the amplitude decrease of both waves following bleaching light adaptation was compared and the mode of the conversion from the late RP to the b-wave was discussed."}
{"id": "PMID:1080216", "title": "The variation in thermoelasticity with sarcomere length in frog's striated muscle.", "content": "The effect of lowering temperature on the resting tension of frog's striated muscle was studied at various sarcomere lengths, using whole muscle, single-fibre and skinned-fibre preparations. And the temperature coefficient of tension, beta=(1/P)(dP/dT) (P, tension; T, temperature) was investigated. In whole sartorius muscles in Ringer solution, beta was negative over the whole range of sarcomere lengths investigated. In the presence of 2 mM procaine the rubber-like thermoelasticity (beta greater than 0) was revealed at medium lengths. At shorter (greater than 2.3 mum) and longer (greater 2.7 mum) lengths, beta stayed negative. In single muscle fibres suspended in Ringer solution with mM procaine, beta was positive over a wider range of lengths (2;3-3.4 mum), and the peak positive value of beta was obtained at the length of 2.6 mum. In skinned fibres suspended in a relaxing solution, beta stayed positive up to the length of 4.2 mum, and the peak value of beta was obtained at the length of 3.0 mum which was considerably longer than in single fibres. The results substantiated A. V. Hill's view on the thermoelasticity of the muscle; there are components with positive beta (contractile apparatus) and those with negative beta (connective tissue and sarcolemma), and at high elongations the positive coefficient is masked by the increasing importance of the negative coefficient.,", "contents": "The variation in thermoelasticity with sarcomere length in frog's striated muscle. The effect of lowering temperature on the resting tension of frog's striated muscle was studied at various sarcomere lengths, using whole muscle, single-fibre and skinned-fibre preparations. And the temperature coefficient of tension, beta=(1/P)(dP/dT) (P, tension; T, temperature) was investigated. In whole sartorius muscles in Ringer solution, beta was negative over the whole range of sarcomere lengths investigated. In the presence of 2 mM procaine the rubber-like thermoelasticity (beta greater than 0) was revealed at medium lengths. At shorter (greater than 2.3 mum) and longer (greater 2.7 mum) lengths, beta stayed negative. In single muscle fibres suspended in Ringer solution with mM procaine, beta was positive over a wider range of lengths (2;3-3.4 mum), and the peak positive value of beta was obtained at the length of 2.6 mum. In skinned fibres suspended in a relaxing solution, beta stayed positive up to the length of 4.2 mum, and the peak value of beta was obtained at the length of 3.0 mum which was considerably longer than in single fibres. The results substantiated A. V. Hill's view on the thermoelasticity of the muscle; there are components with positive beta (contractile apparatus) and those with negative beta (connective tissue and sarcolemma), and at high elongations the positive coefficient is masked by the increasing importance of the negative coefficient.,"}
{"id": "PMID:1080218", "title": "[Carcinofetal antigens. II. Carcinoembryonic antigen (CEA). (author's transl)].", "content": "CEA is a beta1-glycoprotein (mol. w. approx. 200 000) which in embryonic life is usually found as a cell membrane associated antigen in the gastrointestinal (GI) tract and pancreas. Furthermore, it is secreted into body fluids. In healthy adults a very low serum concentration may be found. The clinical significance of CEA lies in its increased formation in primary and secondary adenocarcinomas of colon and rectum and pancreatic carcinoma, where values of 20 ng/ml and more are observed. However, other gastrointestinal (e.g. oesophagus, stomach, gall-bladder) and extragastrointestinal tumors (e.g. lung, breast, urogenital, prostatic, ovarial carcinomas) as well as non-malignant diseases mainly of the GI tract (e.g. hepatitis, cirrhosis, pancreatitis, colitis, diverticulitis) may provoke less frequent and lower increases in the CEA level. Healthy smokers also tend to show a slight increase in CEA concentration. A certain relationship exists between the CEA level and the size and extent of the tumor so that a decrease following operation may account for complete tumor removal, whereas a persistent or recurring increase in the CEA level is highly suspicious of metastases and/or recurrent tumor. Difficulties in proving and purifying CEA are mainly caused by multiple cross-reactions of CEA with other substances, e.g. blood group substances (A, B, Lea, Leb) and normal or other antigens (NGP, NCA, CEX, CCEA 2, NCA 2, CCA-III, FSA, BCGP). The radioimmunoassay is the most suitable method to determine CEA levels in body fluids. The 3 procedures used differ in the precipitation of the specific immune complex by ammonium sulphate (AS), Z-gel (ZG) or a second antibody (SA). Depending on the method, the upper normal limit in serum or plasma corresponds to approximately 2.5 (AS, ZG) or 12.5 (SA) nanogramme/milliliter. CEA determination in the urine is of interest in patients suffering from bladder carcinoma.", "contents": "[Carcinofetal antigens. II. Carcinoembryonic antigen (CEA). (author's transl)]. CEA is a beta1-glycoprotein (mol. w. approx. 200 000) which in embryonic life is usually found as a cell membrane associated antigen in the gastrointestinal (GI) tract and pancreas. Furthermore, it is secreted into body fluids. In healthy adults a very low serum concentration may be found. The clinical significance of CEA lies in its increased formation in primary and secondary adenocarcinomas of colon and rectum and pancreatic carcinoma, where values of 20 ng/ml and more are observed. However, other gastrointestinal (e.g. oesophagus, stomach, gall-bladder) and extragastrointestinal tumors (e.g. lung, breast, urogenital, prostatic, ovarial carcinomas) as well as non-malignant diseases mainly of the GI tract (e.g. hepatitis, cirrhosis, pancreatitis, colitis, diverticulitis) may provoke less frequent and lower increases in the CEA level. Healthy smokers also tend to show a slight increase in CEA concentration. A certain relationship exists between the CEA level and the size and extent of the tumor so that a decrease following operation may account for complete tumor removal, whereas a persistent or recurring increase in the CEA level is highly suspicious of metastases and/or recurrent tumor. Difficulties in proving and purifying CEA are mainly caused by multiple cross-reactions of CEA with other substances, e.g. blood group substances (A, B, Lea, Leb) and normal or other antigens (NGP, NCA, CEX, CCEA 2, NCA 2, CCA-III, FSA, BCGP). The radioimmunoassay is the most suitable method to determine CEA levels in body fluids. The 3 procedures used differ in the precipitation of the specific immune complex by ammonium sulphate (AS), Z-gel (ZG) or a second antibody (SA). Depending on the method, the upper normal limit in serum or plasma corresponds to approximately 2.5 (AS, ZG) or 12.5 (SA) nanogramme/milliliter. CEA determination in the urine is of interest in patients suffering from bladder carcinoma."}
{"id": "PMID:1080223", "title": "Selective arterialization of coronary veins for diffuse coronary occlusion. An experimental evaluation.", "content": "The selective arterialization of coronary veins as a surgical therapy for diffuse coronary arteriosclerosis was studied in canine and sheep experiments. The arterialized blood can be delivered promptly to the selected region of the myocardium and an adequate runoff exists for this mode of revascularization, so that myocardial edema and hemorrhage do not occur. The retrograde blood flow through an anastomosis between the internal mammary artery (IMA) and great cardiac vein (GCV) is excellent, with a predominantly diastolic flow pattern. Follow-up studies indicate significant but partial protection of the myocardium against diffuse LAD occlusion. The size of infarcts is decreased. However, the focal or small transmural infarcts observed are usually located near the apex of the heart. The studies with radioactive microspheres (15 plus or minus 5 mu diameter) revealed that the degree of capillary trapping of microspheres is very much less when the microspheres are injected retrograde into the vein than when injected into the artery. This may indicate that significant \"shunt\" blood flow exists when the coronary vein is arterialized. Thus this study demonstrates both the potential and the limitation of the selective arterialization of coronary veins in revascularizing patients suffering from diffuse coronary arteriosclerosis.", "contents": "Selective arterialization of coronary veins for diffuse coronary occlusion. An experimental evaluation. The selective arterialization of coronary veins as a surgical therapy for diffuse coronary arteriosclerosis was studied in canine and sheep experiments. The arterialized blood can be delivered promptly to the selected region of the myocardium and an adequate runoff exists for this mode of revascularization, so that myocardial edema and hemorrhage do not occur. The retrograde blood flow through an anastomosis between the internal mammary artery (IMA) and great cardiac vein (GCV) is excellent, with a predominantly diastolic flow pattern. Follow-up studies indicate significant but partial protection of the myocardium against diffuse LAD occlusion. The size of infarcts is decreased. However, the focal or small transmural infarcts observed are usually located near the apex of the heart. The studies with radioactive microspheres (15 plus or minus 5 mu diameter) revealed that the degree of capillary trapping of microspheres is very much less when the microspheres are injected retrograde into the vein than when injected into the artery. This may indicate that significant \"shunt\" blood flow exists when the coronary vein is arterialized. Thus this study demonstrates both the potential and the limitation of the selective arterialization of coronary veins in revascularizing patients suffering from diffuse coronary arteriosclerosis."}
{"id": "PMID:1080224", "title": "Resection of acute posterior ventricular aneurysm with repair of ventricular septal defect after acute myocardial infarction.", "content": "Three patients with true posterior myocardial infarctions and ventricular septal defects were treated by posterior infarctectomy, closure of the defect, and appropriate combinations of mitral valve replacement and coronary grafting. Aortic balloon pumping was not used. The technique of infarctectomy and ventricular septal defect closure is illustrated. Two of the 3 patients have excellent long-term results.", "contents": "Resection of acute posterior ventricular aneurysm with repair of ventricular septal defect after acute myocardial infarction. Three patients with true posterior myocardial infarctions and ventricular septal defects were treated by posterior infarctectomy, closure of the defect, and appropriate combinations of mitral valve replacement and coronary grafting. Aortic balloon pumping was not used. The technique of infarctectomy and ventricular septal defect closure is illustrated. Two of the 3 patients have excellent long-term results."}
{"id": "PMID:1080221", "title": "[Genesis of vestibulo-autonomic disorders in space flight].", "content": "Some considerations on the mechanisms of vestibulo-vegetative disturbance in space flight are given on the basis of clinical observations, experience of otoneurological clinic, simulation experiments and examinations of cosmonauts. The starting point in these disturbances was a significant fact of blood redistribution in weightlessness and subsequent changes in hemodynamics, metabolism, etc. The body reactions to weightlessness seem to prepare pronounced vestibulo-vegetative reflexes to threshold and subthreshold vestibular stimuli induced by head and body movements in flight. Some aspects of the vestibular selection and training of cosmonauts are discussed from this point of view.", "contents": "[Genesis of vestibulo-autonomic disorders in space flight]. Some considerations on the mechanisms of vestibulo-vegetative disturbance in space flight are given on the basis of clinical observations, experience of otoneurological clinic, simulation experiments and examinations of cosmonauts. The starting point in these disturbances was a significant fact of blood redistribution in weightlessness and subsequent changes in hemodynamics, metabolism, etc. The body reactions to weightlessness seem to prepare pronounced vestibulo-vegetative reflexes to threshold and subthreshold vestibular stimuli induced by head and body movements in flight. Some aspects of the vestibular selection and training of cosmonauts are discussed from this point of view."}
{"id": "PMID:1080225", "title": "Bridge saphenous vein graft.", "content": "Single aorta-coronary artery vein grafts (bridge grafts) were constructed to two coronary branches with a side-to-side anastomosis in 250 patients. Most of these grafts were constructed between circumflex branches (96 grafts), circumflex and diagnol branches (47 grafts), and anterior descending and diagonol branches (79 grafts). The aim of the bridge graft is to decrease the number of anastomoses, decrease the operative time, and improve graft patency. The hospital mortality rate in this group of patients was 1.2 per cent, and the incidence of postoperative myocardial infarction was 3.6 per cent. One hundred ten patients were restudied after surgery; the average time of restudy was 1 year. Ninety-two grafts of 83.6 per cent had two anastomosis patent; 6 grafts (5.4 per cent) had one anastomosis patent; and in 12 grafts (10.9 per cent), both anastomoses were occluded. One hundred twenty-six associated grafts were studied all the same time; the patency rate was 84.1 per cent. From this experience, we believe the bridge graft is a useful procedure for bypassing the small coronary artery branches.", "contents": "Bridge saphenous vein graft. Single aorta-coronary artery vein grafts (bridge grafts) were constructed to two coronary branches with a side-to-side anastomosis in 250 patients. Most of these grafts were constructed between circumflex branches (96 grafts), circumflex and diagnol branches (47 grafts), and anterior descending and diagonol branches (79 grafts). The aim of the bridge graft is to decrease the number of anastomoses, decrease the operative time, and improve graft patency. The hospital mortality rate in this group of patients was 1.2 per cent, and the incidence of postoperative myocardial infarction was 3.6 per cent. One hundred ten patients were restudied after surgery; the average time of restudy was 1 year. Ninety-two grafts of 83.6 per cent had two anastomosis patent; 6 grafts (5.4 per cent) had one anastomosis patent; and in 12 grafts (10.9 per cent), both anastomoses were occluded. One hundred twenty-six associated grafts were studied all the same time; the patency rate was 84.1 per cent. From this experience, we believe the bridge graft is a useful procedure for bypassing the small coronary artery branches."}
{"id": "PMID:1080226", "title": "Multiple coronary artery aneurysms resulting in myocardial infarction in a young man: treatment by double aorta-coronary saphenous vein bypass grafting.", "content": "A 26-year-old Japanese man was treated for a transmural myocardial infarct caused by multiple aneurysms of the left main (LMC), left anterior descending (LAD), and the right coronary arteries (RCA). He underwent successful double aorta-coronary saphenous vein bypass grafting. The etiology of the aneurysm remains uncertain but an inflammatory origin is most probable. Review of the literature has indicated that this is the seventh case of coronary artery aneurysms without arteriovenous fistulas to be managed by grafting techniques with the saphenous vein. This experience has suggested that young patients presenting with anginal pain or myocardial infarction whould be carefully examined for coronary artery aneurysms. Since most of the patients developed myocardial infarction probably from thrombotic occlusion or embolism of the distal vessel, this lesion should be considered for surgery whenever anatomically feasible. Coronary artery reconstruction by grafting techniques, with or without resection of the aneurysm, is the treatment of choice. Although surgical treatment has provided good clinical amelioration to our patient as well as the patients previoulsy reported, a careful long-term follow-up should be continued for patients with multiple coronary artery aneurysms of doubtful origin.", "contents": "Multiple coronary artery aneurysms resulting in myocardial infarction in a young man: treatment by double aorta-coronary saphenous vein bypass grafting. A 26-year-old Japanese man was treated for a transmural myocardial infarct caused by multiple aneurysms of the left main (LMC), left anterior descending (LAD), and the right coronary arteries (RCA). He underwent successful double aorta-coronary saphenous vein bypass grafting. The etiology of the aneurysm remains uncertain but an inflammatory origin is most probable. Review of the literature has indicated that this is the seventh case of coronary artery aneurysms without arteriovenous fistulas to be managed by grafting techniques with the saphenous vein. This experience has suggested that young patients presenting with anginal pain or myocardial infarction whould be carefully examined for coronary artery aneurysms. Since most of the patients developed myocardial infarction probably from thrombotic occlusion or embolism of the distal vessel, this lesion should be considered for surgery whenever anatomically feasible. Coronary artery reconstruction by grafting techniques, with or without resection of the aneurysm, is the treatment of choice. Although surgical treatment has provided good clinical amelioration to our patient as well as the patients previoulsy reported, a careful long-term follow-up should be continued for patients with multiple coronary artery aneurysms of doubtful origin."}
{"id": "PMID:1080227", "title": "Aneurysm of saphenous vein bypass graft to coronary artery.", "content": "A few cases of aneurysmal dilatation of autogenous vein grafts to peripheral arteries have been reported. However, to our knowledge, no case of this complication in coronary artery vein bypass grafts have been found. We are presenting a case of a vein graft aneurysm which appeared 6 months postoperatively. The patient had had an aortic valve replacement and a vein bypass graft to the right coronary artery.", "contents": "Aneurysm of saphenous vein bypass graft to coronary artery. A few cases of aneurysmal dilatation of autogenous vein grafts to peripheral arteries have been reported. However, to our knowledge, no case of this complication in coronary artery vein bypass grafts have been found. We are presenting a case of a vein graft aneurysm which appeared 6 months postoperatively. The patient had had an aortic valve replacement and a vein bypass graft to the right coronary artery."}
{"id": "PMID:1080228", "title": "Aorta-coronary artery saphenous vein bypass surgery: clinical and angiographic results.", "content": "In a series of 500 consecutive patients undergoing aorta-coronary artery saphenous vein bypass surgery, operative mortality was 2.5% (1% with one-vessel disease, 2% with two-vessel disease, and 3% with three-vessel disease). Transmural myocardial infarction occurred within 30 days after operation in 67 cases (13%) and caused 10 of the 16 deaths in that period. Among 484 patients followed 1 to 58 months (median, 26 months), there have been 20 deaths. Of the 464 survivors, 65% had complete relief of angina, 24% were improved, and 11% were unimproved. In 162 patients who underwent postoperative graft angiography between 1 and 42 months after surgery, a strong positive relationship existed between clinical response and completeness of myocardial revascularization as defined by the presence of a patent graft to all major coronary arteries with greater than 50% stenosis. A similar relationship was found between postoperative multistage treadmill exercise response and completeness of myocardial revascularization. Thus, 82% of patients completely revascularized were asymptomatic at the time of postoperative graft visualization, and 91% had a negative treadmill exercise response.", "contents": "Aorta-coronary artery saphenous vein bypass surgery: clinical and angiographic results. In a series of 500 consecutive patients undergoing aorta-coronary artery saphenous vein bypass surgery, operative mortality was 2.5% (1% with one-vessel disease, 2% with two-vessel disease, and 3% with three-vessel disease). Transmural myocardial infarction occurred within 30 days after operation in 67 cases (13%) and caused 10 of the 16 deaths in that period. Among 484 patients followed 1 to 58 months (median, 26 months), there have been 20 deaths. Of the 464 survivors, 65% had complete relief of angina, 24% were improved, and 11% were unimproved. In 162 patients who underwent postoperative graft angiography between 1 and 42 months after surgery, a strong positive relationship existed between clinical response and completeness of myocardial revascularization as defined by the presence of a patent graft to all major coronary arteries with greater than 50% stenosis. A similar relationship was found between postoperative multistage treadmill exercise response and completeness of myocardial revascularization. Thus, 82% of patients completely revascularized were asymptomatic at the time of postoperative graft visualization, and 91% had a negative treadmill exercise response."}
{"id": "PMID:1080229", "title": "Fluorescence histochemical observations on the uptake and metabolism of tryptophan in the endocrine cells of the hypophysis.", "content": "The present work was undertaken to study the significance of the uptake of precursors of biogenic amines, L-dopa and 5-hydroxytryptophan, in the cells of the pars distalis of the mammalian hypophysis. The results of our own earlier studies suggested that L-dopa and 5-hydroxytryptophan may be taken up by the cells of the pars distalis which possess in their cell membranes the system for active transport of amino acids. In the present study, an effort is made to demonstrate the uptake of the amino acid, tryptophan, which is generally used for the protein synthesis. It was observed that after the administration of tryptophan the small cells of the pars distalis exhibited specific formaldehyde-induced fluorescence characterizing tryptophan and its metabolites. It was also noted that after the administration of tryptophan blue fluorescence was observed in some cells of the pars distalis prior to formaldehyde vapour treatment. The blue fluorescence may be due to the presence of quinoline metabolites of tryptophan formed in the cells. This new observation offers good possibilities to study the metabolism of tryptophan at the cellular level. The results of the present work support the suggestion that L-dopa or 5-hydroxytryptophan is taken up by cells of the pars distalis which possess the mechanism for general active transport of amino acids in the cell membrane.", "contents": "Fluorescence histochemical observations on the uptake and metabolism of tryptophan in the endocrine cells of the hypophysis. The present work was undertaken to study the significance of the uptake of precursors of biogenic amines, L-dopa and 5-hydroxytryptophan, in the cells of the pars distalis of the mammalian hypophysis. The results of our own earlier studies suggested that L-dopa and 5-hydroxytryptophan may be taken up by the cells of the pars distalis which possess in their cell membranes the system for active transport of amino acids. In the present study, an effort is made to demonstrate the uptake of the amino acid, tryptophan, which is generally used for the protein synthesis. It was observed that after the administration of tryptophan the small cells of the pars distalis exhibited specific formaldehyde-induced fluorescence characterizing tryptophan and its metabolites. It was also noted that after the administration of tryptophan blue fluorescence was observed in some cells of the pars distalis prior to formaldehyde vapour treatment. The blue fluorescence may be due to the presence of quinoline metabolites of tryptophan formed in the cells. This new observation offers good possibilities to study the metabolism of tryptophan at the cellular level. The results of the present work support the suggestion that L-dopa or 5-hydroxytryptophan is taken up by cells of the pars distalis which possess the mechanism for general active transport of amino acids in the cell membrane."}
{"id": "PMID:1080251", "title": "Impaired interaction (adhesion-aggregation) of platelets with the subendothelium in storage-pool disease and after aspirin ingestion. A comparison with von Willebrand's disease.", "content": "Possible defects in the interaction of platelets with the subendothelial surface were evaluated in six patients with storeage-pool disease, nine patients with von Willebrand's disease and seven normal subjects who ingested aspirin. Citrated blood was perfused through a chamber containing everted segments of rabbit aorta previously denuded of endothelium by means of a ballon catheter. With normal blood, 83.3 +/- 1.9 per cent (S.E.M.) of the surface was covered by adherent platelets. Platelet adhesion was normal after aspirin ingestion (89.7 +/- 4.6 per cent) and decreased in some patients with storage-pool disease. The most striking defect in both circumstances was the virtual absence of platelet thrombi. In contrast, decreased adhesion (57.3 +/- 3.4 per cent), but normal thrombus formation, was characteristic of von Willebrand's disease. These types of defects in platelet adhesion and aggregation may account for the hemostatic defects in a variety of bleeding disorders. The findings further suggest the possible usefulness of aspirin as an antithrombotic agent.", "contents": "Impaired interaction (adhesion-aggregation) of platelets with the subendothelium in storage-pool disease and after aspirin ingestion. A comparison with von Willebrand's disease. Possible defects in the interaction of platelets with the subendothelial surface were evaluated in six patients with storeage-pool disease, nine patients with von Willebrand's disease and seven normal subjects who ingested aspirin. Citrated blood was perfused through a chamber containing everted segments of rabbit aorta previously denuded of endothelium by means of a ballon catheter. With normal blood, 83.3 +/- 1.9 per cent (S.E.M.) of the surface was covered by adherent platelets. Platelet adhesion was normal after aspirin ingestion (89.7 +/- 4.6 per cent) and decreased in some patients with storage-pool disease. The most striking defect in both circumstances was the virtual absence of platelet thrombi. In contrast, decreased adhesion (57.3 +/- 3.4 per cent), but normal thrombus formation, was characteristic of von Willebrand's disease. These types of defects in platelet adhesion and aggregation may account for the hemostatic defects in a variety of bleeding disorders. The findings further suggest the possible usefulness of aspirin as an antithrombotic agent."}
{"id": "PMID:1080259", "title": "[Paradoxical vestibular reaction in patients with consciousness disorders (nystagmographic recording)].", "content": "In 32 patients with coma of varying intensity and aetiology the effect of light on caloric nystagmus was studied. During consciousness disturbances a light stimulus provoked or increased the intensity of induced caloric nystagmus. This reaction was thus a reverse (paradoxical reaction) of that observed in waking state. Photic stimulation may provoke the rapid phase of nystagmus through facilitating impulses coursing from the subcortical or cortical visual centres to Cajal's nuclei and then through the reticular formation to the vestibulo-oculomotor system. In waking state these impulses are inhibited while in precomatic state they may become prevalent. The paradoxical reaction disappears in deep coma together with all vestibular reactions.", "contents": "[Paradoxical vestibular reaction in patients with consciousness disorders (nystagmographic recording)]. In 32 patients with coma of varying intensity and aetiology the effect of light on caloric nystagmus was studied. During consciousness disturbances a light stimulus provoked or increased the intensity of induced caloric nystagmus. This reaction was thus a reverse (paradoxical reaction) of that observed in waking state. Photic stimulation may provoke the rapid phase of nystagmus through facilitating impulses coursing from the subcortical or cortical visual centres to Cajal's nuclei and then through the reticular formation to the vestibulo-oculomotor system. In waking state these impulses are inhibited while in precomatic state they may become prevalent. The paradoxical reaction disappears in deep coma together with all vestibular reactions."}
{"id": "PMID:1080262", "title": "Evaluation of intracranial disorders in children by computerized transaxial tomography: a preliminary report.", "content": "Computerized transaxial tomography (CTT) is a nonivasive roentgenologic method that demonstrates the configuration of the intracranial contents, including the cerebral parenchyma and the ventricular system. Of 207 children examined by CTT, 53 later had neuroradiologic contrast studies or neurosurgical intervention, or both, and two came to autopsy. The neuroradiologic, surgical, and pathologic findings corresponded with the CTT findings in patients with large ventricles, dupratentorial tumors, intracranial hematomas or cysts, and epidural collections. The correlation was less concordant in patients with intracranial vascular malformations or lesions involving cranial bones. CTT promises to be valuable for diagnosis of many intracranial lesions in children.", "contents": "Evaluation of intracranial disorders in children by computerized transaxial tomography: a preliminary report. Computerized transaxial tomography (CTT) is a nonivasive roentgenologic method that demonstrates the configuration of the intracranial contents, including the cerebral parenchyma and the ventricular system. Of 207 children examined by CTT, 53 later had neuroradiologic contrast studies or neurosurgical intervention, or both, and two came to autopsy. The neuroradiologic, surgical, and pathologic findings corresponded with the CTT findings in patients with large ventricles, dupratentorial tumors, intracranial hematomas or cysts, and epidural collections. The correlation was less concordant in patients with intracranial vascular malformations or lesions involving cranial bones. CTT promises to be valuable for diagnosis of many intracranial lesions in children."}
{"id": "PMID:1080260", "title": "[Diagnostic difficulties in cases of colloidal cysts of the 3rd ventricle].", "content": "The authors report two cases of colloidal cysts of the third ventricle discussing clinical neurological syndromes and diagnostic difficulties in the assessment of pneumoencephalography stressing the value of positive contrast encephalography.", "contents": "[Diagnostic difficulties in cases of colloidal cysts of the 3rd ventricle]. The authors report two cases of colloidal cysts of the third ventricle discussing clinical neurological syndromes and diagnostic difficulties in the assessment of pneumoencephalography stressing the value of positive contrast encephalography."}
{"id": "PMID:1080263", "title": "Oligodendroglial toxicity by serum and lymphocytes from patients with multiple sclerosis.", "content": "The gliotoxic effect of multiple sclerosis serums and lymphocytes was investigated by the measurement of Cr51 release from prelabeled bovine oligodendroglia. There was no difference in glial toxicity between multiple sclerosis patients and normal controls except for a depression of Cr51 release from bovine oligodendroglia in the presence of autologous serum plus lymphocytes in the multiple sclerosis patients compared with the normal controls. This correlated with the phase of the disease and may be due to the presence of a lymphotoxic factor in the active multiple sclerosis serums.", "contents": "Oligodendroglial toxicity by serum and lymphocytes from patients with multiple sclerosis. The gliotoxic effect of multiple sclerosis serums and lymphocytes was investigated by the measurement of Cr51 release from prelabeled bovine oligodendroglia. There was no difference in glial toxicity between multiple sclerosis patients and normal controls except for a depression of Cr51 release from bovine oligodendroglia in the presence of autologous serum plus lymphocytes in the multiple sclerosis patients compared with the normal controls. This correlated with the phase of the disease and may be due to the presence of a lymphotoxic factor in the active multiple sclerosis serums."}
{"id": "PMID:1080264", "title": "Alpha-1-antitrypsin deficiency in New Zealand.", "content": "A severe deficiency of the serum protein alpha-1-antitrypsin can be expected to occur in 1 in 750 European New Zealanders. It can usually be identified by a faint or absent alpha-1 band on serum protein electrophoresis. Forty-seven cases are presented. 31 phenotype ZZ and 16 phenotype SZ. Eighteen have developed emphysema usually by age 40 years, two show childhood liver disease. One adult died of liver disease and three of the emphysema patients had liver abnormalities at post mortem. The remainder, mainly aged less than 30 years, are as yet asymptomatic. Individuals at risk should be protected from respiratory irritants (cigarettes, dusty environments and chest infections) and liver toxins (e.g., alcohol). The partial deficiency state (MZ) which occurs in 4 percent of the population also predisposes to respiratory and perhaps liver disease.", "contents": "Alpha-1-antitrypsin deficiency in New Zealand. A severe deficiency of the serum protein alpha-1-antitrypsin can be expected to occur in 1 in 750 European New Zealanders. It can usually be identified by a faint or absent alpha-1 band on serum protein electrophoresis. Forty-seven cases are presented. 31 phenotype ZZ and 16 phenotype SZ. Eighteen have developed emphysema usually by age 40 years, two show childhood liver disease. One adult died of liver disease and three of the emphysema patients had liver abnormalities at post mortem. The remainder, mainly aged less than 30 years, are as yet asymptomatic. Individuals at risk should be protected from respiratory irritants (cigarettes, dusty environments and chest infections) and liver toxins (e.g., alcohol). The partial deficiency state (MZ) which occurs in 4 percent of the population also predisposes to respiratory and perhaps liver disease."}
{"id": "PMID:1080266", "title": "Effect of methylergonovine on puerperal prolactin secretion.", "content": "Serum prolactin concentration was measured by radioimmunoassay in 29 women, in the first 1 1/2 hours postpartum. Fourteen women received 0.2 mg methylergonovine maleate (Methergine) intramuscularly after the delivery of the placenta. Fifteen women who served as controls received only saline. The rise in serum prolactin concentration seen in the control women (266.4 ng/ml +/- 40.8 SE) was significantly greater than that seen in methylergonovine-treated patients (141.0 ng/ml +/- 29.0. SE).", "contents": "Effect of methylergonovine on puerperal prolactin secretion. Serum prolactin concentration was measured by radioimmunoassay in 29 women, in the first 1 1/2 hours postpartum. Fourteen women received 0.2 mg methylergonovine maleate (Methergine) intramuscularly after the delivery of the placenta. Fifteen women who served as controls received only saline. The rise in serum prolactin concentration seen in the control women (266.4 ng/ml +/- 40.8 SE) was significantly greater than that seen in methylergonovine-treated patients (141.0 ng/ml +/- 29.0. SE)."}
{"id": "PMID:1080269", "title": "Nasotracheal intubation in epiglottitis.", "content": "Significant numbers of patients with epiglottitis have been treated using nasotracheal intubation. Evidence is accumulating that this procedure is safe, effective and reliable when used in this disease. Nasotracheal intubation should be considered a suitable alternative to tracheostomy in epiglottitis.", "contents": "Nasotracheal intubation in epiglottitis. Significant numbers of patients with epiglottitis have been treated using nasotracheal intubation. Evidence is accumulating that this procedure is safe, effective and reliable when used in this disease. Nasotracheal intubation should be considered a suitable alternative to tracheostomy in epiglottitis."}
{"id": "PMID:1080270", "title": "Acetylcholine receptor: modification of synaptic gating mechanism after treatment with a disulfide bond reducing agent.", "content": "Reduction of a 'reactive' disulfide bond in the postsyraptic membrane of the frog neuromuscular junction by dithiothreitol (DTT) decreases both the sensitivity of the membrane to applied acetylcholine (ACh) and the amplitude of the single 'shot effect'. Analysis of ACh induced conductance fluctuations in voltage clamped frog endplates indicates that DTT reduces both the amplitude gamma and duration tau of the elementary conductance events. The mean control value of gamma was 18.5-10(-12) omega-1 with no significant dependence on temperature. The mean control values pi were 2.3 msec at 7-9 degrees C and 0.94 msec at 20-22 degrees C. At 7-9 degrees C 1m7 DTT (20-50 min after application) reduced gamma to 61% of the control value and at 20-22 degrees C to 39%, while tau was reduced to 70% at both temperature ranges. The dose-response curve for iontophoretically applied ACh indicates that neither the total number of ionic channels nor the cooperativity within the receptors are changed. However, the affinity of ACh for the receptor sites was reduced. All effects of DTT were fully reversed by the oxidizing agent 5,5'-dithie-bis-(2-nitro-benzoic acid) (DTNB).", "contents": "Acetylcholine receptor: modification of synaptic gating mechanism after treatment with a disulfide bond reducing agent. Reduction of a 'reactive' disulfide bond in the postsyraptic membrane of the frog neuromuscular junction by dithiothreitol (DTT) decreases both the sensitivity of the membrane to applied acetylcholine (ACh) and the amplitude of the single 'shot effect'. Analysis of ACh induced conductance fluctuations in voltage clamped frog endplates indicates that DTT reduces both the amplitude gamma and duration tau of the elementary conductance events. The mean control value of gamma was 18.5-10(-12) omega-1 with no significant dependence on temperature. The mean control values pi were 2.3 msec at 7-9 degrees C and 0.94 msec at 20-22 degrees C. At 7-9 degrees C 1m7 DTT (20-50 min after application) reduced gamma to 61% of the control value and at 20-22 degrees C to 39%, while tau was reduced to 70% at both temperature ranges. The dose-response curve for iontophoretically applied ACh indicates that neither the total number of ionic channels nor the cooperativity within the receptors are changed. However, the affinity of ACh for the receptor sites was reduced. All effects of DTT were fully reversed by the oxidizing agent 5,5'-dithie-bis-(2-nitro-benzoic acid) (DTNB)."}
{"id": "PMID:1080271", "title": "Chloride transport in isolated skin of Rana esculenta.", "content": "Using a modified Ussing chamber, transepithelial Cl transport in isolated skin of Rana esculenta was determined throughout the year. Unidirectional 36-Cl fluxes were measured in the absence of electrochemical gradients across the skin. The correlations between unidirectional Cl influx and Cl efflux respectively and the Cl concentration in Na containing solutions were not linear in the 0 to 90 meq/1 range. The net Cl influx calculated from these two correlations was, in the same concentration range, also not linearly correlated and was significantly different from zero...", "contents": "Chloride transport in isolated skin of Rana esculenta. Using a modified Ussing chamber, transepithelial Cl transport in isolated skin of Rana esculenta was determined throughout the year. Unidirectional 36-Cl fluxes were measured in the absence of electrochemical gradients across the skin. The correlations between unidirectional Cl influx and Cl efflux respectively and the Cl concentration in Na containing solutions were not linear in the 0 to 90 meq/1 range. The net Cl influx calculated from these two correlations was, in the same concentration range, also not linearly correlated and was significantly different from zero..."}
{"id": "PMID:1080272", "title": "Cerebellar control of vestibular neurons of the frog.", "content": "Intra- and extracellular potentials were recorded from vestibular neurons of the frog following stimulation of the ipsilateral cerebellar cortex (auricular lobe). Single neurons were identified as vestibular neurons by their location within the borders of the vestibular nuclei and by their activation via stimulation of the anterior branch of the VIIIth nerve. 2. Stimulation of the cerebellar auricular lobe provoked in some vestibular neurons apparently pure IPSPs with latencies as short as 1.5 msec suggesting a monosynaptic linkage between Purkinje cells and vestibular neuron. Vestibular-evoked responses were inhibited by cerebellar conditioning stimuli for as long as 50 msec. 3. Cerebellar stimulation also elicited an EPSP-IPSP sequence or occasionally pure EPSPs in vestibular neurons. These EPSPs were probably generated by axon reflex activation via the excitation of axons of primary and secondary vestibular neurons terminating in the cerebellum since they persisted after chronic VIIIth nerve section. Some vestibular evoked resonses were facilitated by cerebellar stimuli for 40-50 msec. 4. Occasionally an inhibitory effect was also induced in vestibular neurons by paired ipsilateral VIIIth nerve stimulation; it's latency of onset and time course suggested that a vestibulo-cerebello-vestibular loop was mainly responsible for the inhibition.", "contents": "Cerebellar control of vestibular neurons of the frog. Intra- and extracellular potentials were recorded from vestibular neurons of the frog following stimulation of the ipsilateral cerebellar cortex (auricular lobe). Single neurons were identified as vestibular neurons by their location within the borders of the vestibular nuclei and by their activation via stimulation of the anterior branch of the VIIIth nerve. 2. Stimulation of the cerebellar auricular lobe provoked in some vestibular neurons apparently pure IPSPs with latencies as short as 1.5 msec suggesting a monosynaptic linkage between Purkinje cells and vestibular neuron. Vestibular-evoked responses were inhibited by cerebellar conditioning stimuli for as long as 50 msec. 3. Cerebellar stimulation also elicited an EPSP-IPSP sequence or occasionally pure EPSPs in vestibular neurons. These EPSPs were probably generated by axon reflex activation via the excitation of axons of primary and secondary vestibular neurons terminating in the cerebellum since they persisted after chronic VIIIth nerve section. Some vestibular evoked resonses were facilitated by cerebellar stimuli for 40-50 msec. 4. Occasionally an inhibitory effect was also induced in vestibular neurons by paired ipsilateral VIIIth nerve stimulation; it's latency of onset and time course suggested that a vestibulo-cerebello-vestibular loop was mainly responsible for the inhibition."}
{"id": "PMID:1080273", "title": "Tonic properties of the Flexor carpi radialis muscle of the male frog (Rana temporaria).", "content": "The mechanical acitvity of the isolated flexor carpi radialis muscle (FCRM) of the mature male frog (Rana temporaria) was compared to that of the sartorius muscle. Muscles were stimulated directly by massive shocks at 20 degrees C and isometric tension was recorded during single twitch, twitch series, long tetani and long tetanus series. Some groups of muscles were poisoned with IAA-N2 or FDNB. The FCRM showed a high resistance to fatigue during a tetanus series and a \"fatigue contracture\" during a twitch series. The rate of the tetanic tension rise was smaller by five to six times than in the sartorius muscle (0 to 50% Po) and the twitch/tetanus ratio was always very small. During continuous activity and after the blocking of recovery metabolic pathways, FCRM used its energetic stores two to three times slower than the sartorius muscle. These stores did not seem to be more important. The results indicate that the FCRM is not a \"tonic\" muscle but, quite possibly, is composed of several types of fibre like many other frog muscles. However, some characteristics of mechanical response cannot be explained by the common classification into two types of fibre.", "contents": "Tonic properties of the Flexor carpi radialis muscle of the male frog (Rana temporaria). The mechanical acitvity of the isolated flexor carpi radialis muscle (FCRM) of the mature male frog (Rana temporaria) was compared to that of the sartorius muscle. Muscles were stimulated directly by massive shocks at 20 degrees C and isometric tension was recorded during single twitch, twitch series, long tetani and long tetanus series. Some groups of muscles were poisoned with IAA-N2 or FDNB. The FCRM showed a high resistance to fatigue during a tetanus series and a \"fatigue contracture\" during a twitch series. The rate of the tetanic tension rise was smaller by five to six times than in the sartorius muscle (0 to 50% Po) and the twitch/tetanus ratio was always very small. During continuous activity and after the blocking of recovery metabolic pathways, FCRM used its energetic stores two to three times slower than the sartorius muscle. These stores did not seem to be more important. The results indicate that the FCRM is not a \"tonic\" muscle but, quite possibly, is composed of several types of fibre like many other frog muscles. However, some characteristics of mechanical response cannot be explained by the common classification into two types of fibre."}
{"id": "PMID:1080274", "title": "Late sodium current in the node of Ranvier.", "content": "Voltage clamp experiments carried out on nodes of Ranvier of myelinated fibres of Rana esculenta showed that a small fractoon of sodium channels fail to inactivate. Thus during long lasting depolarizing pulses there is a small Na-current superimposed on the leakage and potassium currents. This late Na-current appears more marked in sensory fibres than in motor ones.", "contents": "Late sodium current in the node of Ranvier. Voltage clamp experiments carried out on nodes of Ranvier of myelinated fibres of Rana esculenta showed that a small fractoon of sodium channels fail to inactivate. Thus during long lasting depolarizing pulses there is a small Na-current superimposed on the leakage and potassium currents. This late Na-current appears more marked in sensory fibres than in motor ones."}
{"id": "PMID:1080278", "title": "[Sharp's syndrome: a new entity amongst the connective tissue diseases?].", "content": "Sharp and coworkers have coined the name \"Mixed connective tissue disease\" to an association of symptoms seen in different connective tissue diseases. Those symptoms are associated with a special antibody directed against ribonucleoproteins (RNA-P). The presence of this antibody accounts for a high titer of antinuclear fluorescent antibody, of the speckled type. The most frequents symptoms in the association described by Sharp are Raynaud's phenomenon, swollen fingers, non deforming arthritis. We have observed those symptoms without any further symptoms of connective tissue disease, but with the presence of anti-RNA-P antibody. We suggest to coin the name of Sharp's syndrome to that clinical association. The significance of anti-RNA-P, and their correlation with a remarkable benignity of the disease, is discussed.", "contents": "[Sharp's syndrome: a new entity amongst the connective tissue diseases?]. Sharp and coworkers have coined the name \"Mixed connective tissue disease\" to an association of symptoms seen in different connective tissue diseases. Those symptoms are associated with a special antibody directed against ribonucleoproteins (RNA-P). The presence of this antibody accounts for a high titer of antinuclear fluorescent antibody, of the speckled type. The most frequents symptoms in the association described by Sharp are Raynaud's phenomenon, swollen fingers, non deforming arthritis. We have observed those symptoms without any further symptoms of connective tissue disease, but with the presence of anti-RNA-P antibody. We suggest to coin the name of Sharp's syndrome to that clinical association. The significance of anti-RNA-P, and their correlation with a remarkable benignity of the disease, is discussed."}
{"id": "PMID:1080281", "title": "Blue dextran: influence on chromatographic profile and immunoreactivity of human follicle-stimulating hormone (HFSH).", "content": "When FSH is chromatographed on G-100 Sephadex in the absence of Blue Dextran, it elutes as a single peak in the region of monomeric FSH. The addition of Blue Dextran causes a significant portion of FSH, iodinated or uniodinated, to elute in the region of the void volume. When standard curves for FSH are performed under identical conditions, with tracer 125I-FSH from the void volume, and from the FSH monomer region, maximal immunoreactivity was significantly greater for the Blue Dextran-associated tracer.", "contents": "Blue dextran: influence on chromatographic profile and immunoreactivity of human follicle-stimulating hormone (HFSH). When FSH is chromatographed on G-100 Sephadex in the absence of Blue Dextran, it elutes as a single peak in the region of monomeric FSH. The addition of Blue Dextran causes a significant portion of FSH, iodinated or uniodinated, to elute in the region of the void volume. When standard curves for FSH are performed under identical conditions, with tracer 125I-FSH from the void volume, and from the FSH monomer region, maximal immunoreactivity was significantly greater for the Blue Dextran-associated tracer."}
{"id": "PMID:1080282", "title": "Gelfoam embolization of the left gastric artery for bleeding ulcer: experimental considerations.", "content": "A bleeding gastric ulcer was surgically created in 18 dogs, and the left gastric artery was successfully catheterized by percutaneous techniques in 15. Nine of these dogs were treated with vasopressin infusion which did not arrest the hemorrhage. A total of 11 dogs (five of them following unsuccessful vasopressin therapy) underwent embolization with strips of Gelfoam, and hemorrhage stopped in ten. This technique of embolization is concluded to be of value in the management of gastric hemorrhage.", "contents": "Gelfoam embolization of the left gastric artery for bleeding ulcer: experimental considerations. A bleeding gastric ulcer was surgically created in 18 dogs, and the left gastric artery was successfully catheterized by percutaneous techniques in 15. Nine of these dogs were treated with vasopressin infusion which did not arrest the hemorrhage. A total of 11 dogs (five of them following unsuccessful vasopressin therapy) underwent embolization with strips of Gelfoam, and hemorrhage stopped in ten. This technique of embolization is concluded to be of value in the management of gastric hemorrhage."}
{"id": "PMID:1080283", "title": "Release of prostaglandin F1alpha and F2alpha from superfused platelets: quantitative evaluation of the inhibitory effects of some aspirin-like drugs.", "content": "The release of PGF1alpha and PGF2alpha from superfused blood platelets was studied by the combined use of two radioimmunoassay systems with different specificities. PGF1alpha only accounted for approximately 30% of the total immunoreactivity. A substantially similar pattern of release was obtained with platelets of rat and human origin, although the latter released considerably lower amount of both compounds. Indomethacin, Femoprofen, Ditazole and Aspirin all inhibited PGF2alpha release from rat platelets in descending order of potency. Hydrocortisone was partically inactive. The release of PGF1alpha and PGF2alpha was inhibited to the same extent by both Indomethacin and Fenoprofen. Moreover, a quite similar inhibitory effect by the same drug on rat and human platelets was found in preliminary experiments. In agreement with a previous similar finding, Aspirin displayed a higher inhibitory activity than that reported in other tissues. The use of superfused platelets seems to provide a simple and reproducible model for studying pharmacologic influences upon PG formation.", "contents": "Release of prostaglandin F1alpha and F2alpha from superfused platelets: quantitative evaluation of the inhibitory effects of some aspirin-like drugs. The release of PGF1alpha and PGF2alpha from superfused blood platelets was studied by the combined use of two radioimmunoassay systems with different specificities. PGF1alpha only accounted for approximately 30% of the total immunoreactivity. A substantially similar pattern of release was obtained with platelets of rat and human origin, although the latter released considerably lower amount of both compounds. Indomethacin, Femoprofen, Ditazole and Aspirin all inhibited PGF2alpha release from rat platelets in descending order of potency. Hydrocortisone was partically inactive. The release of PGF1alpha and PGF2alpha was inhibited to the same extent by both Indomethacin and Fenoprofen. Moreover, a quite similar inhibitory effect by the same drug on rat and human platelets was found in preliminary experiments. In agreement with a previous similar finding, Aspirin displayed a higher inhibitory activity than that reported in other tissues. The use of superfused platelets seems to provide a simple and reproducible model for studying pharmacologic influences upon PG formation."}
{"id": "PMID:1080284", "title": "Cold-haemagglutinin disease with an autoantibody exhibiting different specificities at different temperatures.", "content": "A case of chronic cold-haemagglutinin disease is reported in which an agglutinin apparently carrying two specificities was present. At first, specificity was anti-not-I and anti-I respectively: in the course of time anti-I was replaced by anti-A-1. Both anti-I and anti-A-1 could be demonstrated at room temperature only while specificity was anti-not-I at 4 degrees C. The antibody displayed haemolytic activity at room temperature and gave stronger reactions when treated biphasically. The same pattern of specificity was apparent in haemolysis tests, i.e. anti-not-I in the biphasical reaction 4 degrees C goes to 37 degrees C and first anti-I and later anti-A-1 at room temperature and at 22 degrees C goes to 37 degrees C. Anti-A-1 was not neutralized by A bloodgroup substance of animal origin nor by secretor saliva.", "contents": "Cold-haemagglutinin disease with an autoantibody exhibiting different specificities at different temperatures. A case of chronic cold-haemagglutinin disease is reported in which an agglutinin apparently carrying two specificities was present. At first, specificity was anti-not-I and anti-I respectively: in the course of time anti-I was replaced by anti-A-1. Both anti-I and anti-A-1 could be demonstrated at room temperature only while specificity was anti-not-I at 4 degrees C. The antibody displayed haemolytic activity at room temperature and gave stronger reactions when treated biphasically. The same pattern of specificity was apparent in haemolysis tests, i.e. anti-not-I in the biphasical reaction 4 degrees C goes to 37 degrees C and first anti-I and later anti-A-1 at room temperature and at 22 degrees C goes to 37 degrees C. Anti-A-1 was not neutralized by A bloodgroup substance of animal origin nor by secretor saliva."}
{"id": "PMID:1080285", "title": "Antibody response by cultured spleen fragments from carrier-primed mice to hapten-protein conjugates.", "content": "Hapten-protein conjugates stimulated very poor anti-hapten responses in mouse spleen fragment cultures from unimmunized mice, whereas hapten coupled to type III pneumococcal polysaccharide or polylysine induced good responses. When the donors of the fragments were primed with the carrier protein, hapten-protein conjugates induced a strong anti-hapten response. Both the true primary and the carrier-primed response in vitro consisted mainly of IgA antibodies of 9-13S. In carrier-primed responses also IgM was produced at the beginning and IgG at the end of those responses.", "contents": "Antibody response by cultured spleen fragments from carrier-primed mice to hapten-protein conjugates. Hapten-protein conjugates stimulated very poor anti-hapten responses in mouse spleen fragment cultures from unimmunized mice, whereas hapten coupled to type III pneumococcal polysaccharide or polylysine induced good responses. When the donors of the fragments were primed with the carrier protein, hapten-protein conjugates induced a strong anti-hapten response. Both the true primary and the carrier-primed response in vitro consisted mainly of IgA antibodies of 9-13S. In carrier-primed responses also IgM was produced at the beginning and IgG at the end of those responses."}
{"id": "PMID:1080286", "title": "Effects of treating immune lymphocytes with a proteolytic enzyme (papain): reduction in immune potential and recovery after incubation.", "content": "Immune spleen cells were treated with papain, washed, and boosted with homologous antigen (4-hydroxy-3-iodo-5-nitrophenylacetic acid coupled to chicken globulin) for 1 hr at 4 degrees C. When transferred to irradiated mice, they produced up to 20-fold less anti-hapten antibody than non-papain-treated cells. The lymphocytes could recover their ability to respond if incubated at 37 degrees C after papain treatment but before antigen boosting. Incubation for 30 min at 37 degrees C gave complete or almost complete recovery. Although papain usually reduced the response, in 31% of experiments the reduction was not significant. Possible reasons for this variability are discussed.", "contents": "Effects of treating immune lymphocytes with a proteolytic enzyme (papain): reduction in immune potential and recovery after incubation. Immune spleen cells were treated with papain, washed, and boosted with homologous antigen (4-hydroxy-3-iodo-5-nitrophenylacetic acid coupled to chicken globulin) for 1 hr at 4 degrees C. When transferred to irradiated mice, they produced up to 20-fold less anti-hapten antibody than non-papain-treated cells. The lymphocytes could recover their ability to respond if incubated at 37 degrees C after papain treatment but before antigen boosting. Incubation for 30 min at 37 degrees C gave complete or almost complete recovery. Although papain usually reduced the response, in 31% of experiments the reduction was not significant. Possible reasons for this variability are discussed."}
{"id": "PMID:1080287", "title": "Genetic control of the murine cell-mediated immune response in vivo. I. H-2-linked responsiveness to the terpolymer L-glutamic acid-60-L-alanine-30-L-tyrosine-10.", "content": "Various inbred and congenic strains of mice were immunized with the linear terpolymer L-glutamic acid-60-L-alanine-30-L-tyrosine-10 (GAT). Using a radioisotopic footpad assay to measure cell-mediated immunity in vivo, mice with H-2-a, H-2-b, H-2-d, and H-2-k histocompatibility alleles showed a positive reaction, whereas mice with H-2p and H-2-s alleles failed to respond. The ability of \"responder\" lymphocytes to show an immune response resides in the thymus-derived (T) lymphocyte population. Unfractionated spleen cells from H-2-q nonresponder mice, on transfer, showed no reactivity, whereas T-cell-enriched preparations were active.", "contents": "Genetic control of the murine cell-mediated immune response in vivo. I. H-2-linked responsiveness to the terpolymer L-glutamic acid-60-L-alanine-30-L-tyrosine-10. Various inbred and congenic strains of mice were immunized with the linear terpolymer L-glutamic acid-60-L-alanine-30-L-tyrosine-10 (GAT). Using a radioisotopic footpad assay to measure cell-mediated immunity in vivo, mice with H-2-a, H-2-b, H-2-d, and H-2-k histocompatibility alleles showed a positive reaction, whereas mice with H-2p and H-2-s alleles failed to respond. The ability of \"responder\" lymphocytes to show an immune response resides in the thymus-derived (T) lymphocyte population. Unfractionated spleen cells from H-2-q nonresponder mice, on transfer, showed no reactivity, whereas T-cell-enriched preparations were active."}
{"id": "PMID:1080288", "title": "Surface markers on human B and T lymphocytes. VII. Rosette formation between peripheral T lymphocytes and lymphoblastoid B-cell lines.", "content": "Almost 100% of peripheral T lymphocytes are shown to have the capacity to form rosettes with human lymphoblastoid B-cell lines, predominantly at 4 degrees C and with lines having surface-bound IgG. Blast-transformed T cells retained this capacity and formed rosettes even at 37 degrees C. Unstimulated T cells bound less readily to B-cell blasts, stimulated by pokeweed mitogen for 72 hr. Even though rosettes, formed at 4 degrees C, were stable for several hours at 37 degrees C, no T-cell-mediated cytotoxicity could be detected during overnight incubation. Extreme pH values and trypsinization decreased rosette formation, whereas neuraminidase treatment enhanced the reaction. Rosette formation was independent of bivalent cations and unimpaired in the presence of inhibitors (NaF, NaN3), undiluted human or fetal calf sera, protein A, sonicated sheep erythrocyte membranes, and normal or heat-aggregated human IgG. Anti-Ig, anti-beta2-microglobulin, or anti-T cell sera did not influence rosette formation.", "contents": "Surface markers on human B and T lymphocytes. VII. Rosette formation between peripheral T lymphocytes and lymphoblastoid B-cell lines. Almost 100% of peripheral T lymphocytes are shown to have the capacity to form rosettes with human lymphoblastoid B-cell lines, predominantly at 4 degrees C and with lines having surface-bound IgG. Blast-transformed T cells retained this capacity and formed rosettes even at 37 degrees C. Unstimulated T cells bound less readily to B-cell blasts, stimulated by pokeweed mitogen for 72 hr. Even though rosettes, formed at 4 degrees C, were stable for several hours at 37 degrees C, no T-cell-mediated cytotoxicity could be detected during overnight incubation. Extreme pH values and trypsinization decreased rosette formation, whereas neuraminidase treatment enhanced the reaction. Rosette formation was independent of bivalent cations and unimpaired in the presence of inhibitors (NaF, NaN3), undiluted human or fetal calf sera, protein A, sonicated sheep erythrocyte membranes, and normal or heat-aggregated human IgG. Anti-Ig, anti-beta2-microglobulin, or anti-T cell sera did not influence rosette formation."}
{"id": "PMID:1080289", "title": "The protective role of thymus-derived lymphocytes in arbovirus-induced meningoencephalitis.", "content": "The inflammatory response in mouse brain and the elimination of virus from the brain following intracerebral inoculation of a group A arbovirus has been shown to be T-cell dependent. Virus persists in the brain of T-cell depleted mice, and inflammation is depressed. Virus persists in the brain of T-cell depleted mice, and inflammation is depressed. Inflammation is restored by transfer of immune cells but not by immune serum, and the transferred cells are effective in reducing virus titres in the absence of circulating antibody. Transferred antibody is not efficient in protecting infected mice. The cells that restore inflammation can come from mice immunized with a group A arbovirus of the same subtype but not from mice immunized with more distantly related viruses. Macrophages may be important effector cells working in collaboration with T cells.", "contents": "The protective role of thymus-derived lymphocytes in arbovirus-induced meningoencephalitis. The inflammatory response in mouse brain and the elimination of virus from the brain following intracerebral inoculation of a group A arbovirus has been shown to be T-cell dependent. Virus persists in the brain of T-cell depleted mice, and inflammation is depressed. Virus persists in the brain of T-cell depleted mice, and inflammation is depressed. Inflammation is restored by transfer of immune cells but not by immune serum, and the transferred cells are effective in reducing virus titres in the absence of circulating antibody. Transferred antibody is not efficient in protecting infected mice. The cells that restore inflammation can come from mice immunized with a group A arbovirus of the same subtype but not from mice immunized with more distantly related viruses. Macrophages may be important effector cells working in collaboration with T cells."}
{"id": "PMID:1080290", "title": "Are fetal cells in maternal blood mainly B lymphocytes?", "content": "In 10 blood samples from mothers who were studied after the delivery of a male infant and who had Y-body-containing cells in their circulations (mean value, 0.06%) the proportions of surface-Ig- and Y-body-positive lymphocytes were determined after passage through nylon wool. In each case one part of the sample was treated with pronase before the nylon wool incubation and the other was not. Passage through nylon wool of maternal lymphocytes not treated with pronase resulted in a drastic decrease of fetal cells, since only 1 Y-body-containing cell was found in 1 of the 10 maternal blood samples studied, as compared with 28 Y bodies in the 10 samples not passed through nylon wool. In lymphocytes pretreated with pronase, 7 fetal cells were recovered after passage through nylon wool, although only in 5 of the 10 samples. Thus most fetal cells in maternal blood adhere to nylon wool, as do B lymphocytes, but this adherence is to some extent affected by pronase incubation.", "contents": "Are fetal cells in maternal blood mainly B lymphocytes? In 10 blood samples from mothers who were studied after the delivery of a male infant and who had Y-body-containing cells in their circulations (mean value, 0.06%) the proportions of surface-Ig- and Y-body-positive lymphocytes were determined after passage through nylon wool. In each case one part of the sample was treated with pronase before the nylon wool incubation and the other was not. Passage through nylon wool of maternal lymphocytes not treated with pronase resulted in a drastic decrease of fetal cells, since only 1 Y-body-containing cell was found in 1 of the 10 maternal blood samples studied, as compared with 28 Y bodies in the 10 samples not passed through nylon wool. In lymphocytes pretreated with pronase, 7 fetal cells were recovered after passage through nylon wool, although only in 5 of the 10 samples. Thus most fetal cells in maternal blood adhere to nylon wool, as do B lymphocytes, but this adherence is to some extent affected by pronase incubation."}
{"id": "PMID:1080291", "title": "Interactions between ricinus agglutinin and human IgM and IgG.", "content": "Ricinus agglutinin, purified until homogeneous, precipitates serum glycoproteins with terminal nonreducing galactose residues including IgM and IgG. Almost 100% of IgM reacted with anti-IgM and ricinus agglutinin in quantitative precipitin tests. In similar tests, almost 100% of polyclonal IgG was precipitated by excess anti-IgG, whereas only about 10% reacted with ricinus agglutinin. In quantitative precipitin analyses and affinity chromatography experiments with insolubilized ricinus agglutinin and isolated monoclonal IgG1 and IgG3 proteins, only IgG3 proteins reacted with ricinus agglutinin.", "contents": "Interactions between ricinus agglutinin and human IgM and IgG. Ricinus agglutinin, purified until homogeneous, precipitates serum glycoproteins with terminal nonreducing galactose residues including IgM and IgG. Almost 100% of IgM reacted with anti-IgM and ricinus agglutinin in quantitative precipitin tests. In similar tests, almost 100% of polyclonal IgG was precipitated by excess anti-IgG, whereas only about 10% reacted with ricinus agglutinin. In quantitative precipitin analyses and affinity chromatography experiments with insolubilized ricinus agglutinin and isolated monoclonal IgG1 and IgG3 proteins, only IgG3 proteins reacted with ricinus agglutinin."}
{"id": "PMID:1080292", "title": "Deficient lymphoid cell-mediated, PHA-induced cytotoxicity in rheumatoid arthritis patients.", "content": "Lymphoid cells from patients with rheumatoid arthritis were compared with those from healthy blood donors and from nonrheumatoid arthritis patients for the ability to manifest in vitro cytotoxicity against target cells in the presence of phytohemagglutinin (PHA) or anti-target cell antibodies. The PHA-induced cytotoxicity in the rheumatoid patient group was significantly lower than that of the blood donors (P less than 0.01) and of the nonrheumatoid patients (P less than 0.05). The rheumatoid arthritis patients appeared to fall into two groups, one with normal and one with distinctly subnormal PHA-induced cytotoxicity. No obvious differences were observed betes, or the proportion in peripheral blood of T lymphocytes (E-RFC) or Fc-receptor-bearing lymphocytes (EA-RFC). There were no significant differences between the groups with regard to antibody-dependent cytotoxicity.", "contents": "Deficient lymphoid cell-mediated, PHA-induced cytotoxicity in rheumatoid arthritis patients. Lymphoid cells from patients with rheumatoid arthritis were compared with those from healthy blood donors and from nonrheumatoid arthritis patients for the ability to manifest in vitro cytotoxicity against target cells in the presence of phytohemagglutinin (PHA) or anti-target cell antibodies. The PHA-induced cytotoxicity in the rheumatoid patient group was significantly lower than that of the blood donors (P less than 0.01) and of the nonrheumatoid patients (P less than 0.05). The rheumatoid arthritis patients appeared to fall into two groups, one with normal and one with distinctly subnormal PHA-induced cytotoxicity. No obvious differences were observed betes, or the proportion in peripheral blood of T lymphocytes (E-RFC) or Fc-receptor-bearing lymphocytes (EA-RFC). There were no significant differences between the groups with regard to antibody-dependent cytotoxicity."}
{"id": "PMID:1080293", "title": "[Hemophilia A: early detection of women carriers by Laurell's quantitative immunoelectrophoresis].", "content": "Using one-stage quantitative factor VIII assay and the quantitative immunoelectrophoretic method of LAURELL a good correlation has been found between factor VII activity and protein concentration. In 80% of the known hemophilia A carriers the protein concentration was about twice as high as in normals as compared to the factor VIII activity, whereas about 20% of the known carriers have the same ratio between factor VIII protein and factor VIII activity as normals. The reliability of the test and its usefulness for the detection of hemophilia A carriers are discussed.", "contents": "[Hemophilia A: early detection of women carriers by Laurell's quantitative immunoelectrophoresis]. Using one-stage quantitative factor VIII assay and the quantitative immunoelectrophoretic method of LAURELL a good correlation has been found between factor VII activity and protein concentration. In 80% of the known hemophilia A carriers the protein concentration was about twice as high as in normals as compared to the factor VIII activity, whereas about 20% of the known carriers have the same ratio between factor VIII protein and factor VIII activity as normals. The reliability of the test and its usefulness for the detection of hemophilia A carriers are discussed."}
{"id": "PMID:1080294", "title": "[Alpha 1 antitrypsin deficiency. Physiopathological consequences].", "content": "Alpha-1-antitrypsine, a glycoprotein synthesized in the liver, exists in different phenotypic variants. The Z allele, when heterozygotic, causes a diminution of protein in the blood; when homozygotic it causes an almost complete absence of blood protein. It appears that in instances of deficiency the hepatocyte is unable to secrete the protein it synthesizes, and this leads to intracellular accumulation of protein (visible on histological examination as granules of amorphic P.A.S.-positive material). The deficiency is accompanied by pulmonary manifestations of the destructive emphysematic type. The lesions are probably related to the absence of anti-protease activity in the lung tissues: proteolysis of the lung in animals reproduces the lesions observed in man. Studies on large populations have shown that, in people exhibiting a total or intermediate deficiency in alpha-1-antitrypsine, cigarette smoking represents a significant risk factor in that it increases the probability of parenchylmal destruction.", "contents": "[Alpha 1 antitrypsin deficiency. Physiopathological consequences]. Alpha-1-antitrypsine, a glycoprotein synthesized in the liver, exists in different phenotypic variants. The Z allele, when heterozygotic, causes a diminution of protein in the blood; when homozygotic it causes an almost complete absence of blood protein. It appears that in instances of deficiency the hepatocyte is unable to secrete the protein it synthesizes, and this leads to intracellular accumulation of protein (visible on histological examination as granules of amorphic P.A.S.-positive material). The deficiency is accompanied by pulmonary manifestations of the destructive emphysematic type. The lesions are probably related to the absence of anti-protease activity in the lung tissues: proteolysis of the lung in animals reproduces the lesions observed in man. Studies on large populations have shown that, in people exhibiting a total or intermediate deficiency in alpha-1-antitrypsine, cigarette smoking represents a significant risk factor in that it increases the probability of parenchylmal destruction."}
{"id": "PMID:1080295", "title": "[Experimental studies on the vascular effect of adrenaline and POR-8 infiltrations in the jaw region].", "content": "The infiltration of a vasoconstrictor causes bleeding to diminish in the surgical field. Adrenalin is such a vasoconstrictor, but because of its metabolic action, acidosis develops in the tissue followed by decreased oxygen tension, a reactive hyperemia. This can of course cause postoperative hemorrage and edema.", "contents": "[Experimental studies on the vascular effect of adrenaline and POR-8 infiltrations in the jaw region]. The infiltration of a vasoconstrictor causes bleeding to diminish in the surgical field. Adrenalin is such a vasoconstrictor, but because of its metabolic action, acidosis develops in the tissue followed by decreased oxygen tension, a reactive hyperemia. This can of course cause postoperative hemorrage and edema."}
{"id": "PMID:1080296", "title": "Epidemiology and aetiology of cancer of the uterine cervix including the detection of carcinogenic N-nitrosamines in the human vaginal vault.", "content": "Cancer of the uterine cervix occurs with high frequency in black females of several populations of Southern Africa. It accounts for 40-50% of all cancers in Black females in the Johannesburg area and for over 40% of those in women in the rural lowveld area of the Transvaal. The aetiology of this cancer has several distinct features, almost all of which concern some or other aspect of coitus. Age at first coitus and at first marriage, and promiscuity, are very strongly associated with the development of the disease, and age, race, religion, culture, socio-economic status, extent of genital hygiene and venereal disease also play a major role. In spite of current belief, circumcision of the male partner is at best weakly associated with the development of the disease, and so are menstruation, coital habits and pregnancy. Certain aetiological features are selectively discussed; these include Herpesvirus type II, 'rogue' spermatozoa and the recent isolation of a group of potent carcinogens, the nitrosamines, in infected discharge samples taken from the vaginal vault of patients attending gynaecological clinics.", "contents": "Epidemiology and aetiology of cancer of the uterine cervix including the detection of carcinogenic N-nitrosamines in the human vaginal vault. Cancer of the uterine cervix occurs with high frequency in black females of several populations of Southern Africa. It accounts for 40-50% of all cancers in Black females in the Johannesburg area and for over 40% of those in women in the rural lowveld area of the Transvaal. The aetiology of this cancer has several distinct features, almost all of which concern some or other aspect of coitus. Age at first coitus and at first marriage, and promiscuity, are very strongly associated with the development of the disease, and age, race, religion, culture, socio-economic status, extent of genital hygiene and venereal disease also play a major role. In spite of current belief, circumcision of the male partner is at best weakly associated with the development of the disease, and so are menstruation, coital habits and pregnancy. Certain aetiological features are selectively discussed; these include Herpesvirus type II, 'rogue' spermatozoa and the recent isolation of a group of potent carcinogens, the nitrosamines, in infected discharge samples taken from the vaginal vault of patients attending gynaecological clinics."}
{"id": "PMID:1080297", "title": "Tuberculosis control in an urban Black community.", "content": "A tuberculosis case-finding survey of an urban Black community is described. The programme included health education of the community leaders, tuberculin testing of all scholars at the schools, and of the pre-school and non-schoolgoi  ng children in their homes. The insignificant reactors to the tuberculin skin test were BCG-vaccinated. Isoniazid prophylactic therapy of the significant reactors was instituted and their contacts were radiologically investigated. The various results are to be found in the tables in the main body of this review.", "contents": "Tuberculosis control in an urban Black community. A tuberculosis case-finding survey of an urban Black community is described. The programme included health education of the community leaders, tuberculin testing of all scholars at the schools, and of the pre-school and non-schoolgoi  ng children in their homes. The insignificant reactors to the tuberculin skin test were BCG-vaccinated. Isoniazid prophylactic therapy of the significant reactors was instituted and their contacts were radiologically investigated. The various results are to be found in the tables in the main body of this review."}
{"id": "PMID:1080299", "title": "Plasma expansion in surgical patients with high central venous pressure (CVP); the relationship of blood volume to hematocrit, CVP, pulmonary wedge pressure, and cardiorespiratory changes.", "content": "There was no correlation of blood volume measurements with central venous pressure (CVP) or hematocrit determinations and only minimal suggestive trends with wedge pressure in a large series of postoperative patients; the lack of correlations emphasize the unreliability of venous pressure and hematocrit determinations to predict blood volume alterations. To evaluate the physiological problems, to define optimal therapeutic goals, and to measure therapeutic effectiveness of volume loading with an oncotically active agent, we measured the hemodynamic and oxygen transport responses to 500 ml. of 5 percent albumin given over 1 hour in 22 patients with CVP greater than 15 cm. H2O. The patients were separated into two groups according to the CVP response to volume therapy. The CVP decreased in 14 (64 percent) of these patients (Group 1), but it increased slightly but not significantly in eight (36 percent) patients (Group 2). In Group 1 patients, there was increased flow, improvement of tissue perfusion as reflected by increased oxygen consumption, and augmentation of the ventricular function. In Group 2 there were slight increases in mean flow, mean pulmonary arterial pressure, and mean transit time and slightly decreased pulmonary vascular resistance; there was appreciable improvement in left ventricular function without significant deterioration of right ventricular function. The high initial central venous pressure is not a reliable index of either hypervolemia or cardiac failure in critically ill patients. It is concluded that a trial of volume loading with an oncotically active agent with frequent auscultation of the chest and careful observation of the CVP trends will give the maximum diagnostic as well as therapeutic information.", "contents": "Plasma expansion in surgical patients with high central venous pressure (CVP); the relationship of blood volume to hematocrit, CVP, pulmonary wedge pressure, and cardiorespiratory changes. There was no correlation of blood volume measurements with central venous pressure (CVP) or hematocrit determinations and only minimal suggestive trends with wedge pressure in a large series of postoperative patients; the lack of correlations emphasize the unreliability of venous pressure and hematocrit determinations to predict blood volume alterations. To evaluate the physiological problems, to define optimal therapeutic goals, and to measure therapeutic effectiveness of volume loading with an oncotically active agent, we measured the hemodynamic and oxygen transport responses to 500 ml. of 5 percent albumin given over 1 hour in 22 patients with CVP greater than 15 cm. H2O. The patients were separated into two groups according to the CVP response to volume therapy. The CVP decreased in 14 (64 percent) of these patients (Group 1), but it increased slightly but not significantly in eight (36 percent) patients (Group 2). In Group 1 patients, there was increased flow, improvement of tissue perfusion as reflected by increased oxygen consumption, and augmentation of the ventricular function. In Group 2 there were slight increases in mean flow, mean pulmonary arterial pressure, and mean transit time and slightly decreased pulmonary vascular resistance; there was appreciable improvement in left ventricular function without significant deterioration of right ventricular function. The high initial central venous pressure is not a reliable index of either hypervolemia or cardiac failure in critically ill patients. It is concluded that a trial of volume loading with an oncotically active agent with frequent auscultation of the chest and careful observation of the CVP trends will give the maximum diagnostic as well as therapeutic information."}
{"id": "PMID:1080300", "title": "Plasma components which interfere with ristocetin-induced platelet aggregation.", "content": "Normal human plasma contains a component or components which interfere with ristocetin-induced platelet aggregation. Preliminary examination suggests a protein (or proteins) which binds ristocetin and competes more effectively for ristocetin than do the proteins involved in ristocetin-induced platelet aggregation. The presence of this protein in normal human plasma also prevents ristocetin-induced precipitation of plasma proteins at levels of ristocetin necessary to produce platelet aggregation (0.5-2.0 mg/ml). Serum contains an apparent two0fold increase of this component when compared with plasma. Heating serum at 56 degrees for one hour results in ad additional 2 to 4 forl increase. The presence of a ristocetin-binding protein in normal human plasma requires that this protein be saturated with ristocetin before ristocetin-induced platelet aggregation will occur. Variations in the ristocetin-binding protein(s) will cause apparent discrepancies in ristocetin-induced platelet aggregation in normal human plasmas.", "contents": "Plasma components which interfere with ristocetin-induced platelet aggregation. Normal human plasma contains a component or components which interfere with ristocetin-induced platelet aggregation. Preliminary examination suggests a protein (or proteins) which binds ristocetin and competes more effectively for ristocetin than do the proteins involved in ristocetin-induced platelet aggregation. The presence of this protein in normal human plasma also prevents ristocetin-induced precipitation of plasma proteins at levels of ristocetin necessary to produce platelet aggregation (0.5-2.0 mg/ml). Serum contains an apparent two0fold increase of this component when compared with plasma. Heating serum at 56 degrees for one hour results in ad additional 2 to 4 forl increase. The presence of a ristocetin-binding protein in normal human plasma requires that this protein be saturated with ristocetin before ristocetin-induced platelet aggregation will occur. Variations in the ristocetin-binding protein(s) will cause apparent discrepancies in ristocetin-induced platelet aggregation in normal human plasmas."}
{"id": "PMID:1080302", "title": "The antiglobulin microcytotoxicity assay in HL-A genotyped families.", "content": "HL-A genotyping was accomplished in 30 families (8 black, 21 white, and 1 American Indian) based on serological results obtained by our ususal lymphocytotoxicity assays. Each family was further tested by an antiglobulin microcytotoxicity method. Segregation patterns obtained by the latter method, compared with those of the former, showed few discrepancies. HL-A-identical siblings were serologically similar by the antiglobulin assay with frequency of discordant reactions at the same level as that of our regular two-stage lymphocytotoxicity assay. The antiglobulin method was shown to be highly reproducible with 1.3% discordance between duplicate tests. Many sera employed in routine cytotoxicity testing gave positive reactions with all family members in the antiglobulin assay (39% all positive by antiglobulin versus 16% by the regular cytotoxicity test), so that this method has limited usefulness for routine HL-A typing. The antiglobulin assay may have particular value, however, for identifying HL-A haplotypes in families, especially non-Caucasian, which give infrequent positive reactions in the usual lymphocytotoxicity assays.", "contents": "The antiglobulin microcytotoxicity assay in HL-A genotyped families. HL-A genotyping was accomplished in 30 families (8 black, 21 white, and 1 American Indian) based on serological results obtained by our ususal lymphocytotoxicity assays. Each family was further tested by an antiglobulin microcytotoxicity method. Segregation patterns obtained by the latter method, compared with those of the former, showed few discrepancies. HL-A-identical siblings were serologically similar by the antiglobulin assay with frequency of discordant reactions at the same level as that of our regular two-stage lymphocytotoxicity assay. The antiglobulin method was shown to be highly reproducible with 1.3% discordance between duplicate tests. Many sera employed in routine cytotoxicity testing gave positive reactions with all family members in the antiglobulin assay (39% all positive by antiglobulin versus 16% by the regular cytotoxicity test), so that this method has limited usefulness for routine HL-A typing. The antiglobulin assay may have particular value, however, for identifying HL-A haplotypes in families, especially non-Caucasian, which give infrequent positive reactions in the usual lymphocytotoxicity assays."}
{"id": "PMID:1080303", "title": "Ag-F: serological and genetic identification of a new locus in the rat governing lymphocyte membrane antigens.", "content": "A new genetic locus in the rat (Ag-F) is described. It controls expression of lymphocyte membrane antigens as determined by allospecific cytotoxic antibody. It is linked by approximately two recombination units to the gene for albinism and is therefore in linkage group I. It is suggested that Ag-F is the homologue in the rat of mouse H-1. It appears to be highly polymorphic and yields antigens which are highly cross reactive serologically. Disparity at Af-F is the probable cause of alloantibody responses attributable to reciprocal immunization between the Lewis and Fischer strains.", "contents": "Ag-F: serological and genetic identification of a new locus in the rat governing lymphocyte membrane antigens. A new genetic locus in the rat (Ag-F) is described. It controls expression of lymphocyte membrane antigens as determined by allospecific cytotoxic antibody. It is linked by approximately two recombination units to the gene for albinism and is therefore in linkage group I. It is suggested that Ag-F is the homologue in the rat of mouse H-1. It appears to be highly polymorphic and yields antigens which are highly cross reactive serologically. Disparity at Af-F is the probable cause of alloantibody responses attributable to reciprocal immunization between the Lewis and Fischer strains."}
{"id": "PMID:1080305", "title": "[Effect of insulin of frog skeletal muscle respiration].", "content": "The respiration of the isolated frog sartorius muscle was measured polarographically. The endogenous respiration was seen to increase by 8 and 6 times in 20 mM KCL and 0.05 mM 2,4-DNP, resp., and by 40 percent in 10mM pyruvate or lactate. Insulin stimulated the endogemous respiration from 27 to 38 mm-3 O2/hr. per gramm of wet weight. In the excess of exogenous lactate and pyruvate, the degree of the insulin stimulated respiration did not change. It is suggested that the increase of the endogenous respiration by insulin cannot be accounted for by the mobilization of the substrate of glycolysis.", "contents": "[Effect of insulin of frog skeletal muscle respiration]. The respiration of the isolated frog sartorius muscle was measured polarographically. The endogenous respiration was seen to increase by 8 and 6 times in 20 mM KCL and 0.05 mM 2,4-DNP, resp., and by 40 percent in 10mM pyruvate or lactate. Insulin stimulated the endogemous respiration from 27 to 38 mm-3 O2/hr. per gramm of wet weight. In the excess of exogenous lactate and pyruvate, the degree of the insulin stimulated respiration did not change. It is suggested that the increase of the endogenous respiration by insulin cannot be accounted for by the mobilization of the substrate of glycolysis."}
{"id": "PMID:1080306", "title": "[Transport of D-xylose in isolated frog sartorius muscle in the presence of actinomycin D].", "content": "The action of actinomycin D on the basal and insulin stimulated D-xylose uptake has been studied on isolated frog sartorius muscles. Actinomycin D at concentrations of 10 mg/ml or 100 mg/ml exerts no effect on the basal transport of sugar and causes no alteration in the response to insulin. The distribution coefficient of actinomycin D in muscle tissue is equal 0,9--1. It is supposed that the inhibition of RNA synthesis by antibiotics does not influence sugar transport.", "contents": "[Transport of D-xylose in isolated frog sartorius muscle in the presence of actinomycin D]. The action of actinomycin D on the basal and insulin stimulated D-xylose uptake has been studied on isolated frog sartorius muscles. Actinomycin D at concentrations of 10 mg/ml or 100 mg/ml exerts no effect on the basal transport of sugar and causes no alteration in the response to insulin. The distribution coefficient of actinomycin D in muscle tissue is equal 0,9--1. It is supposed that the inhibition of RNA synthesis by antibiotics does not influence sugar transport."}
{"id": "PMID:1080309", "title": "[Rare causes of acute gastric hemorrhage].", "content": "According to the author's findings rare causes of acute gastric hemorrhages (the Mallory-Weiss syndrome, cancerous lesions of the gastrointestinal organs, excluding the stomach, esophageal and duodenal diverticulum, prolapse of gastric mucosa into the duodenum, necrosis of the gastric wall, its traumatic rupture, the Rendu-Osler disease, rupture of the renal artery aneurysm) made 3.2% of other causes. Profuse hemorrhage was noted in 12 of 32 patients under consideration. In 8 of 32 patients (25%) the diagnosis was established only at autopsy. In other more frequent causes of acute gastric hemorrhage the diagnosis proved to be feasible in 90-95% of cases.", "contents": "[Rare causes of acute gastric hemorrhage]. According to the author's findings rare causes of acute gastric hemorrhages (the Mallory-Weiss syndrome, cancerous lesions of the gastrointestinal organs, excluding the stomach, esophageal and duodenal diverticulum, prolapse of gastric mucosa into the duodenum, necrosis of the gastric wall, its traumatic rupture, the Rendu-Osler disease, rupture of the renal artery aneurysm) made 3.2% of other causes. Profuse hemorrhage was noted in 12 of 32 patients under consideration. In 8 of 32 patients (25%) the diagnosis was established only at autopsy. In other more frequent causes of acute gastric hemorrhage the diagnosis proved to be feasible in 90-95% of cases."}
{"id": "PMID:1080310", "title": "[Changes in the level of electrolytes in the blood of patients with acute gastro-intestinal hemorrhages].", "content": "The authors have studied kinetically the content of potassium, sodium, calcium and chlorides in blood plasma, potassium and sodium in erythrocytes and diurnal urine in 27 practically healthy subjects and in 100 patients with acute gastrointestinal hemorrhages of various etiology. Variations in the level of electrolytes are described.", "contents": "[Changes in the level of electrolytes in the blood of patients with acute gastro-intestinal hemorrhages]. The authors have studied kinetically the content of potassium, sodium, calcium and chlorides in blood plasma, potassium and sodium in erythrocytes and diurnal urine in 27 practically healthy subjects and in 100 patients with acute gastrointestinal hemorrhages of various etiology. Variations in the level of electrolytes are described."}
{"id": "PMID:1080311", "title": "[Emergency esophagogastroduodenoscopy in the diagnosis of acute gastrointestinal hemorrhage].", "content": "It is reported that among 1383 endoscopies, performed in the clinic, 618--were defined as urgent ones for acute gastrointestinal hemorrhages. There were 470 male and 148 female patients, their age ranging from 14 to 93 years. 408 endoscopies were performed during the first 6 hours since patients' admission to the clinic, and 210--during the following 48 hours. Contraindications to an urgent exploration of patients in hemorrhage from upper portions of the digestive tract are determined.", "contents": "[Emergency esophagogastroduodenoscopy in the diagnosis of acute gastrointestinal hemorrhage]. It is reported that among 1383 endoscopies, performed in the clinic, 618--were defined as urgent ones for acute gastrointestinal hemorrhages. There were 470 male and 148 female patients, their age ranging from 14 to 93 years. 408 endoscopies were performed during the first 6 hours since patients' admission to the clinic, and 210--during the following 48 hours. Contraindications to an urgent exploration of patients in hemorrhage from upper portions of the digestive tract are determined."}
{"id": "PMID:1080308", "title": "Hypertensive optic neuropathy.", "content": "In 1970 a 62-year-old physician with hypertensive vascular disease suffered a small infarction in the left optic disc, which left him with a subtle paracentral temporal visual field defect in that eye. In 1973 he had another separate and distinct episode in the same eye, which produced a dense lower nasal field defect. Careful Hruby lens examination of the disc under high magnification revealed focal arteriolar disease in the optic nerve head corresponding to the field defects, and fluorescein angiography confirmed these findings. The importance of differentiating ischemic optic neuropathy, hypertensive optic neuropathy, and temporal arteritis with optic nerve involvement is emphasized, and the therapy of each is discussed.", "contents": "Hypertensive optic neuropathy. In 1970 a 62-year-old physician with hypertensive vascular disease suffered a small infarction in the left optic disc, which left him with a subtle paracentral temporal visual field defect in that eye. In 1973 he had another separate and distinct episode in the same eye, which produced a dense lower nasal field defect. Careful Hruby lens examination of the disc under high magnification revealed focal arteriolar disease in the optic nerve head corresponding to the field defects, and fluorescein angiography confirmed these findings. The importance of differentiating ischemic optic neuropathy, hypertensive optic neuropathy, and temporal arteritis with optic nerve involvement is emphasized, and the therapy of each is discussed."}
{"id": "PMID:1080313", "title": "Metabolism of 1-(3-trifluoromethylphenyl)-3-(2-hydroxyethyl) quinazoline-2,4(1H,3H)-dione (H-88). I. Species differences in metabolism.", "content": "1. Following oral administration of [14C] H-88 to rat, mouse, quinea-pig and hamster, 40-65% and 5-15% of radioactivity was excreted in urine and faeces respectively during 3 or 4 days. In rabbit, monkey and man, more than 80% of radioactivity was excreted in urine during 2 or 3 days, and faecal excretion was negligible. 2. Rabbit, rat or guinea-pig excreted non-labelled H-88 in urine as unchanged H-88 (M-I), 1-(3-trifluoromethylphenyl) quinazoline-2,4(1H,3H)-dione-3-acetic acid (M-II), H-88 glucuronide (M-III) and 1-(3-trifluoromethylphenyl)-3-(2-hydroxyethyl)-6-hydroxyguinazoline-2,4(1H,3H)-dione (M-IV). 3. The carboxylic acid (M-II) was the major metabolite of H-88 in rat, mouse, guinea-pig and hamster urine and faeces, while the major metabolite in urine of rabbit, monkey and man was H-88 glucuronide (M-III). The 6-hydroxy compound (M-IV) was a major metabolite only in guinea-pig.", "contents": "Metabolism of 1-(3-trifluoromethylphenyl)-3-(2-hydroxyethyl) quinazoline-2,4(1H,3H)-dione (H-88). I. Species differences in metabolism. 1. Following oral administration of [14C] H-88 to rat, mouse, quinea-pig and hamster, 40-65% and 5-15% of radioactivity was excreted in urine and faeces respectively during 3 or 4 days. In rabbit, monkey and man, more than 80% of radioactivity was excreted in urine during 2 or 3 days, and faecal excretion was negligible. 2. Rabbit, rat or guinea-pig excreted non-labelled H-88 in urine as unchanged H-88 (M-I), 1-(3-trifluoromethylphenyl) quinazoline-2,4(1H,3H)-dione-3-acetic acid (M-II), H-88 glucuronide (M-III) and 1-(3-trifluoromethylphenyl)-3-(2-hydroxyethyl)-6-hydroxyguinazoline-2,4(1H,3H)-dione (M-IV). 3. The carboxylic acid (M-II) was the major metabolite of H-88 in rat, mouse, guinea-pig and hamster urine and faeces, while the major metabolite in urine of rabbit, monkey and man was H-88 glucuronide (M-III). The 6-hydroxy compound (M-IV) was a major metabolite only in guinea-pig."}
{"id": "PMID:1080318", "title": "[Condition of lymphoid tissue and characteristics of development of antitoxin immunity during an experimental course of methotrexate treatment].", "content": "Methotrexate administered to albino mice in a dose of 0.5 mg/kg for a period of 10 days caused significant (two period) changes in the central and peripheral immunocompetent tissue. During the first--destructive--period there occurred a marked reduction in the weight of the spleen and the thymus, a cut in the count of the nuclear cells in them, and also a significant depletion of the cellular composition of the lymphoid organs. Suspension of the cytostatic was followed by a gradual restoration of all the indices under study, slower in the thymus than in the spleen. In the animals immunized with the tetanus toxoid against the background of the methotrexate administration all the changes in the lymphoid organs developed under a preponderant influence of the cytostatic agent; the action of the antigen was depressed during the whole experiment. Specific antibodies appeared in the blood of these animals later and their titres were lower than in control; experimental mice were also less resistant to tetanus toxin.", "contents": "[Condition of lymphoid tissue and characteristics of development of antitoxin immunity during an experimental course of methotrexate treatment]. Methotrexate administered to albino mice in a dose of 0.5 mg/kg for a period of 10 days caused significant (two period) changes in the central and peripheral immunocompetent tissue. During the first--destructive--period there occurred a marked reduction in the weight of the spleen and the thymus, a cut in the count of the nuclear cells in them, and also a significant depletion of the cellular composition of the lymphoid organs. Suspension of the cytostatic was followed by a gradual restoration of all the indices under study, slower in the thymus than in the spleen. In the animals immunized with the tetanus toxoid against the background of the methotrexate administration all the changes in the lymphoid organs developed under a preponderant influence of the cytostatic agent; the action of the antigen was depressed during the whole experiment. Specific antibodies appeared in the blood of these animals later and their titres were lower than in control; experimental mice were also less resistant to tetanus toxin."}
{"id": "PMID:1080319", "title": "Fatal haemorrhage following atraumatic liver rupture secondary to post-operative perforation of the gall-bladder. A case report.", "content": "A case of post-operative perforation of the gallbladder with massive intraperitoneal haemorrhage is presented. The clinical picture was complicated by urinary infection and bleeding from an acute gastric erosion. It is suggested that the antibiotic treatment given for his urinary infection may have altered the clinical course of his cholecystitis, allowing a silent perforation to take place and present in the form of an atraumatic liver rupture.", "contents": "Fatal haemorrhage following atraumatic liver rupture secondary to post-operative perforation of the gall-bladder. A case report. A case of post-operative perforation of the gallbladder with massive intraperitoneal haemorrhage is presented. The clinical picture was complicated by urinary infection and bleeding from an acute gastric erosion. It is suggested that the antibiotic treatment given for his urinary infection may have altered the clinical course of his cholecystitis, allowing a silent perforation to take place and present in the form of an atraumatic liver rupture."}
{"id": "PMID:1080317", "title": "[Cellular organization of the vestibular nucleus of the frog medulla oblongata].", "content": "Ventral vestibular nucleus of frogs is composed by multipolar cells of isodendritic type. Three basic kinds of cells are found in the nucleus-large, medium and small ones which differ mainly by the number of synaptic contacts on their perikaryon. The neuropile of the vestibular nucleus contains a large number of unmyelinated nervous fibers and exhibits numerous contacts formed along each of the fibers. Similar features in the organization of ventral vestibular nucleus in frogs and Deuters nucleus in rats and cats are noted. Functional role of cellular elements composing the nucleus is discussed.", "contents": "[Cellular organization of the vestibular nucleus of the frog medulla oblongata]. Ventral vestibular nucleus of frogs is composed by multipolar cells of isodendritic type. Three basic kinds of cells are found in the nucleus-large, medium and small ones which differ mainly by the number of synaptic contacts on their perikaryon. The neuropile of the vestibular nucleus contains a large number of unmyelinated nervous fibers and exhibits numerous contacts formed along each of the fibers. Similar features in the organization of ventral vestibular nucleus in frogs and Deuters nucleus in rats and cats are noted. Functional role of cellular elements composing the nucleus is discussed."}
{"id": "PMID:1080321", "title": "The distribution of polymorphic traits in atopic asthmatic patients.", "content": "The phenotypic distribution and gene frequencies of haptoglobin (Hp), transferrin (Tf), group specific component (Gc), cholinesterase (Cho E2), and alpha1-antitrypsin (Pi) in plasma proteins, and phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase ((6-PGD), esterase D (Es D), phosphohexose isomerase (PHI), adenosine deaminase (ADA) and acid phosphatase (AcP) in red cells were studied in 127 atopic, asthmatic patients. The gene frequencies were compared with normal groups. The phenotypic distribution of the Pi system in atopic patients was somewhat different from the normal. No significant differences were found between the two groups in protein systems or in enzyme systems, except Pi systems. In conclusion, except for the Pi system, no definite association between polymorphic traits and atopic asthma was found in this study.", "contents": "The distribution of polymorphic traits in atopic asthmatic patients. The phenotypic distribution and gene frequencies of haptoglobin (Hp), transferrin (Tf), group specific component (Gc), cholinesterase (Cho E2), and alpha1-antitrypsin (Pi) in plasma proteins, and phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase ((6-PGD), esterase D (Es D), phosphohexose isomerase (PHI), adenosine deaminase (ADA) and acid phosphatase (AcP) in red cells were studied in 127 atopic, asthmatic patients. The gene frequencies were compared with normal groups. The phenotypic distribution of the Pi system in atopic patients was somewhat different from the normal. No significant differences were found between the two groups in protein systems or in enzyme systems, except Pi systems. In conclusion, except for the Pi system, no definite association between polymorphic traits and atopic asthma was found in this study."}
{"id": "PMID:1080322", "title": "The fine structure of the lymphocyte nucleus under conditions of phytohaemagglutinin stimulation.", "content": "The nucleus of PHA (phytohaemagglutinin) cultured lymphocytes is studied by EDTA, Thalium-Schiff and uranyl-lead techniques. Scattering of the dense chromatin, increase in the number of NB (nuclear bodies) and nucleolar modifications are established. Transportation is seen of granules similar to perichromatin ones from the nucleus into the cytoplasm. Morphometric investigation reveals a decrease between 7,7 and 26,3% of the dense chromatin and also that the number of PCG (perichromatin granules) to be the greatest in the untransformed PHA lymphocytes, e.g. B lymphocytes. It is established in new born chickens that the lymphocytes of the bourse of Fabricius are richer in PCG than these of the thymus.", "contents": "The fine structure of the lymphocyte nucleus under conditions of phytohaemagglutinin stimulation. The nucleus of PHA (phytohaemagglutinin) cultured lymphocytes is studied by EDTA, Thalium-Schiff and uranyl-lead techniques. Scattering of the dense chromatin, increase in the number of NB (nuclear bodies) and nucleolar modifications are established. Transportation is seen of granules similar to perichromatin ones from the nucleus into the cytoplasm. Morphometric investigation reveals a decrease between 7,7 and 26,3% of the dense chromatin and also that the number of PCG (perichromatin granules) to be the greatest in the untransformed PHA lymphocytes, e.g. B lymphocytes. It is established in new born chickens that the lymphocytes of the bourse of Fabricius are richer in PCG than these of the thymus."}
{"id": "PMID:1080323", "title": "Observation of Kolmer's crystalloid outside the retina. Presence in the corneal endothelium in various conditions.", "content": "A structure similar to Kolmer's crystalloid, an organelle found in the horizontal cells of the retina, was observed in endothelial cells of the cornea from four patients (one case of hyperparathyroidism and three cases of corneal dystrophy). Based on a morphological analysis it is concluded that the structures in the present cases are identical with the cytoplasmic structure of the horizontal cell, and that the membranous tubes probably constitute a special form of rough surfaced endoplasmic reticulum, eventually involved in transport and/or a specialized protein synthesis.", "contents": "Observation of Kolmer's crystalloid outside the retina. Presence in the corneal endothelium in various conditions. A structure similar to Kolmer's crystalloid, an organelle found in the horizontal cells of the retina, was observed in endothelial cells of the cornea from four patients (one case of hyperparathyroidism and three cases of corneal dystrophy). Based on a morphological analysis it is concluded that the structures in the present cases are identical with the cytoplasmic structure of the horizontal cell, and that the membranous tubes probably constitute a special form of rough surfaced endoplasmic reticulum, eventually involved in transport and/or a specialized protein synthesis."}
{"id": "PMID:1080325", "title": "Complement fixing and lymphocytotoxic antibodies in serum of pregnant women at delivery. III. Clinical observations.", "content": "The investigation comprised 2,008 pregnancies. Sera fnd lymphocytotoxic antibodies (LCTAb). 14 women had only CFPAb, 44 had CFPAb and LCTAb, 224 had only LCTAb. In 3 of 481 cord blood serum samples LCTAb were demonstrated. No cases of neutropenia or thrombocytopenia was observed. No association was found between CFPAb or LCTAb and stillbirth, birth defects, degree of bilirubinaemia, diseases of the newborn, or placental weight. It was suggested that the applied lymphocytotoxicity test detects both in vivo cytolytic antibodies and enhancing antibodies, which may explain the results.", "contents": "Complement fixing and lymphocytotoxic antibodies in serum of pregnant women at delivery. III. Clinical observations. The investigation comprised 2,008 pregnancies. Sera fnd lymphocytotoxic antibodies (LCTAb). 14 women had only CFPAb, 44 had CFPAb and LCTAb, 224 had only LCTAb. In 3 of 481 cord blood serum samples LCTAb were demonstrated. No cases of neutropenia or thrombocytopenia was observed. No association was found between CFPAb or LCTAb and stillbirth, birth defects, degree of bilirubinaemia, diseases of the newborn, or placental weight. It was suggested that the applied lymphocytotoxicity test detects both in vivo cytolytic antibodies and enhancing antibodies, which may explain the results."}
{"id": "PMID:1080326", "title": "Electron microscopy of Trichomonas vaginalis Donn\u00e9: interaction with vaginal epithelium in human trichomoniasis.", "content": "Light and electron microscopic examination of portio biopsies from eleven patients with trichomoniasis vaginalis revealed in four patients clusters of cells of T. vaginalis (T. vag.) which were attached to the vaginal mucosa. The minimum gap between adjacent trichomonad cells was the size of gap junctions (2 nm). Cells of T. vag. invaded superficially located epithelial cells but did not penetrate to the deeper cell layers of the epithelium. The latter, however, were frequently infiltrated with neutrophilic granulocytes. Contact between cells of T. vag. and neutrophils was not observed. Trichomonads which were attached to epithelial cells contained a dense network of cytoplasmic microfilaments in the part of the cell which came into contact with the epithelium. The remaining part contained the organelles normally seen in T. vag.. Endocytotic cell activity of amoeboid T. vag. occurred from the free cell surface only. A cell coat on the cell membrane--formed by bristles--was confined mainly to pinocytotic invaginations. Glycogen granules which were absent from the larger part of the epithelium were densely packed in the trichomonad cells. The findings in this study indicate that the interaction between the cells of T. vag. and the vaginal epithelium takes place primarily at a distance probably by means of substances released into the vaginal fluid, and secondly by a direct cell contact mechanism.", "contents": "Electron microscopy of Trichomonas vaginalis Donn\u00e9: interaction with vaginal epithelium in human trichomoniasis. Light and electron microscopic examination of portio biopsies from eleven patients with trichomoniasis vaginalis revealed in four patients clusters of cells of T. vaginalis (T. vag.) which were attached to the vaginal mucosa. The minimum gap between adjacent trichomonad cells was the size of gap junctions (2 nm). Cells of T. vag. invaded superficially located epithelial cells but did not penetrate to the deeper cell layers of the epithelium. The latter, however, were frequently infiltrated with neutrophilic granulocytes. Contact between cells of T. vag. and neutrophils was not observed. Trichomonads which were attached to epithelial cells contained a dense network of cytoplasmic microfilaments in the part of the cell which came into contact with the epithelium. The remaining part contained the organelles normally seen in T. vag.. Endocytotic cell activity of amoeboid T. vag. occurred from the free cell surface only. A cell coat on the cell membrane--formed by bristles--was confined mainly to pinocytotic invaginations. Glycogen granules which were absent from the larger part of the epithelium were densely packed in the trichomonad cells. The findings in this study indicate that the interaction between the cells of T. vag. and the vaginal epithelium takes place primarily at a distance probably by means of substances released into the vaginal fluid, and secondly by a direct cell contact mechanism."}
{"id": "PMID:1080324", "title": "Stimulation of the vestibular receptor by means of step temperature changes during continuous aural irrigation.", "content": "A technique for rapid, balanced hot/cold stimulation of the vestibular receptor is presented. During continuous aural irrigation the temperature of the irrigation fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. As a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. Application of the test to 32 clinical subjects demonstrated that the heat conduction model and the analysis used in timing the sequence of thermal pulses was accurate. We expect that, with further refinements, the new technique will prove superior to conventional caloric test methods in the detection and measurement of subtle as well as gross abnormalities of the vestibular system.", "contents": "Stimulation of the vestibular receptor by means of step temperature changes during continuous aural irrigation. A technique for rapid, balanced hot/cold stimulation of the vestibular receptor is presented. During continuous aural irrigation the temperature of the irrigation fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. As a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. Application of the test to 32 clinical subjects demonstrated that the heat conduction model and the analysis used in timing the sequence of thermal pulses was accurate. We expect that, with further refinements, the new technique will prove superior to conventional caloric test methods in the detection and measurement of subtle as well as gross abnormalities of the vestibular system."}
{"id": "PMID:1080340", "title": "Ionic requirements for rapid axonal transport in vitro in frog sciatic nerves.", "content": "The effects of K+, Na+, hypo- and hypertonicity on the synthesis and fast axonal transport of 3H-leucine-labelled protein were studied in vitro in the frog sciatic system. The methodology used made it possible to discriminate between effects on synthesis and transport of protein. The preparation which consisted of the dorsal ganglia, the sciatic nerve and the gastrocnemius muscle was placed in an incubation chamber. The ganglia were incubated in standard Ringer containing 3H-leucine and the nerve was prefused with modified Ringer. Perfusion of the nerve for 17 h with K+-free Ringer of Na+-free Ringer did not affect the rapid axonal transport of 3H-leucine-labelled material from the ganglia along the nerve towards a ligature in front of which it accumulated. Not was the transport influenced by concentrations of K+ up to 68.8 mM. In contrast concentrations exceeding 100 mM K+ partially inhibited the transport. Inhibition by ouabain (0.1 mM) was not prevented by elevating K+ to 30 mM, Deviation from isotonicity, towards a hypo- or a hypertonic medium, partially inhibited axonal transport. The transport inhibitory effects showed reversibility. Expermintal conditions, which arrested the transport, were tested in spearate experiments for effects on uptake of 3H-leucine into TCA-soluble and insoluble ganglionic components. K+ substituted for Na+, ouabain (0.1 mM) and hypotonic Ringer partially inhibited the amino acid uptake but also subsequent steps in the incorporation process, whereas only the latter was inhibited by hypertonic Ringer. The results are discussed in relation to possible changes in energy metabolism.", "contents": "Ionic requirements for rapid axonal transport in vitro in frog sciatic nerves. The effects of K+, Na+, hypo- and hypertonicity on the synthesis and fast axonal transport of 3H-leucine-labelled protein were studied in vitro in the frog sciatic system. The methodology used made it possible to discriminate between effects on synthesis and transport of protein. The preparation which consisted of the dorsal ganglia, the sciatic nerve and the gastrocnemius muscle was placed in an incubation chamber. The ganglia were incubated in standard Ringer containing 3H-leucine and the nerve was prefused with modified Ringer. Perfusion of the nerve for 17 h with K+-free Ringer of Na+-free Ringer did not affect the rapid axonal transport of 3H-leucine-labelled material from the ganglia along the nerve towards a ligature in front of which it accumulated. Not was the transport influenced by concentrations of K+ up to 68.8 mM. In contrast concentrations exceeding 100 mM K+ partially inhibited the transport. Inhibition by ouabain (0.1 mM) was not prevented by elevating K+ to 30 mM, Deviation from isotonicity, towards a hypo- or a hypertonic medium, partially inhibited axonal transport. The transport inhibitory effects showed reversibility. Expermintal conditions, which arrested the transport, were tested in spearate experiments for effects on uptake of 3H-leucine into TCA-soluble and insoluble ganglionic components. K+ substituted for Na+, ouabain (0.1 mM) and hypotonic Ringer partially inhibited the amino acid uptake but also subsequent steps in the incorporation process, whereas only the latter was inhibited by hypertonic Ringer. The results are discussed in relation to possible changes in energy metabolism."}
{"id": "PMID:1080341", "title": "Conductance recording of ionic outflow from frog skin glands during nerve stimulation.", "content": "A method for continuous recording of the ionic efflux from frog skin glands has been developed which under given experimental conditions provides a convenient index on glandular secretion. A nerve skin preparation from the calf is mounted so that the outside of the skin forms the bottom of a small test compartment with distilled water while the corium side is bathing in Ringer's solution. After determination of the adequate nerve stimulus parameters for discernible gland secretion to occur, the skin nerve was stimulated at 10 Hz during varying periods and chemical control analyses performed of the changes in ionic content of the test compartment solution. The main ionic outflow consisted of Na+ and Cl- in equal amounts, the outflow of other ions during stimulation being negligible. The concomitant conductance changes were measured as variations of absorption in a high-frequency field applied to a conductance probe placed in the test chamber. Determinations of the NaCl outflow based on the conductance changes were in good agreement with the values obtained by chemical analysis. The continuous conductance recordings proved to give approximative information of the quantities and time relations of the glandular secretion, allowing a direct comparison with, e.g., skin potential changes.", "contents": "Conductance recording of ionic outflow from frog skin glands during nerve stimulation. A method for continuous recording of the ionic efflux from frog skin glands has been developed which under given experimental conditions provides a convenient index on glandular secretion. A nerve skin preparation from the calf is mounted so that the outside of the skin forms the bottom of a small test compartment with distilled water while the corium side is bathing in Ringer's solution. After determination of the adequate nerve stimulus parameters for discernible gland secretion to occur, the skin nerve was stimulated at 10 Hz during varying periods and chemical control analyses performed of the changes in ionic content of the test compartment solution. The main ionic outflow consisted of Na+ and Cl- in equal amounts, the outflow of other ions during stimulation being negligible. The concomitant conductance changes were measured as variations of absorption in a high-frequency field applied to a conductance probe placed in the test chamber. Determinations of the NaCl outflow based on the conductance changes were in good agreement with the values obtained by chemical analysis. The continuous conductance recordings proved to give approximative information of the quantities and time relations of the glandular secretion, allowing a direct comparison with, e.g., skin potential changes."}
{"id": "PMID:1080342", "title": "Host specificity of DNA in Haemophilus influenzae: the in vivo action of the restriction endonucleases on phage and bacterial DNA.", "content": "In Haemophilus influenzae strains only the type 1 of the restriction endonucleases have an in vivo effect on phage and bacterial transforming DNA. The type 2 of restriction endonucleases which act very efficiently in vitro are completely inactive in vivo. The reasons for this inactivity is unknown.", "contents": "Host specificity of DNA in Haemophilus influenzae: the in vivo action of the restriction endonucleases on phage and bacterial DNA. In Haemophilus influenzae strains only the type 1 of the restriction endonucleases have an in vivo effect on phage and bacterial transforming DNA. The type 2 of restriction endonucleases which act very efficiently in vitro are completely inactive in vivo. The reasons for this inactivity is unknown."}
{"id": "PMID:1080344", "title": "Value of internal mammary artery bypass.", "content": "Coronary artery bypass in 150 patients with angina has been accomplished with 225 IMA grafts and 85 saphenous vein grafts. There were 7 (4.7%) hospital deaths. Posterative catheterization in 85% of patients revealed that 9 (4.7%) IMA grafts and 11 (15%) vein graft had thrombosed. One-year catheterization in 32 patients (38 IMA grafts and 13 vein grafts) revealed no additional graft failures. The better patency of the IMA grafts appears to be related to their smaller size and therfore greater velocity of blood flow and freedom from intimal proliferation.", "contents": "Value of internal mammary artery bypass. Coronary artery bypass in 150 patients with angina has been accomplished with 225 IMA grafts and 85 saphenous vein grafts. There were 7 (4.7%) hospital deaths. Posterative catheterization in 85% of patients revealed that 9 (4.7%) IMA grafts and 11 (15%) vein graft had thrombosed. One-year catheterization in 32 patients (38 IMA grafts and 13 vein grafts) revealed no additional graft failures. The better patency of the IMA grafts appears to be related to their smaller size and therfore greater velocity of blood flow and freedom from intimal proliferation."}
{"id": "PMID:1080347", "title": "Objective elaluation of bypass surgery in patients with acute coronary artery disease.", "content": "80 patients with acute coronary artery disease, including 70 patients with PIA, 8 patients with acute myocardial infarction, and 2 patients with cardiogenic shock, underwent bypass surgery. The surgical mortality rate was 1.4% in patients with PIA and during an average follow-up period of 15 months none of the patients died of cardiac disease. Ventricular function, as indicated by systolic ejection fraction and segmental wall motion, was noted to improve in many patients. Postoperative studies showed the internal mammary artery to be superior to the saphenous vein graft for left coronary bypass procedures. This was demonstrated by 100% patency of internal mammary artery grafts to the left anterior descending coronary artery. We believe that when a patient presents with one of the syndromes of acute coronary artery disease such as unstable angina, severe chest pain suggestive of infarction without infarction, status anginosis, recurrent ventricular tachycardia or acute myocardial infarction complicated by evidence of potential extension, cardiogenic shock, heart block or rupture, these patients deserve at least coronary and left ventricular  cineangiographic studies with hemodynamic evaluation. Our experience to date suggests that myocardial revascularization and catheterization carry a lesser risk than that of medical treatment.", "contents": "Objective elaluation of bypass surgery in patients with acute coronary artery disease. 80 patients with acute coronary artery disease, including 70 patients with PIA, 8 patients with acute myocardial infarction, and 2 patients with cardiogenic shock, underwent bypass surgery. The surgical mortality rate was 1.4% in patients with PIA and during an average follow-up period of 15 months none of the patients died of cardiac disease. Ventricular function, as indicated by systolic ejection fraction and segmental wall motion, was noted to improve in many patients. Postoperative studies showed the internal mammary artery to be superior to the saphenous vein graft for left coronary bypass procedures. This was demonstrated by 100% patency of internal mammary artery grafts to the left anterior descending coronary artery. We believe that when a patient presents with one of the syndromes of acute coronary artery disease such as unstable angina, severe chest pain suggestive of infarction without infarction, status anginosis, recurrent ventricular tachycardia or acute myocardial infarction complicated by evidence of potential extension, cardiogenic shock, heart block or rupture, these patients deserve at least coronary and left ventricular  cineangiographic studies with hemodynamic evaluation. Our experience to date suggests that myocardial revascularization and catheterization carry a lesser risk than that of medical treatment."}
{"id": "PMID:1080348", "title": "Early and late effects of the coronary bypass operation on cardiac contractility and coordination.", "content": "In 100 cases of coronary heart disease, cardiac contractility was studied before and after coronary bypass by a flow method, the force ballistocardiogram, and by a pressure method, the carotid pulse derivative. The two methods gave very similar results in most cases, and the agreement of their averages was impressive. A marked increase in cardiac strength, reaching its maximum a month or two after operation, followed the procedure in most cases. Cardiac coordination was also improved at this time in many subjects, but complete cardiac normality was very rarely attained. This early postoperative improvement was greater in hearts judged to be weak before operation than in those judged to be normal in strength. The early cardiac improvement was seldom held in its entirety, but usually declined at a rate, which, if continued, would abolish the postoperative improvement in about three years. This rate of cardiac decline was many times faster than that found in healthy persons or in unoperated cases of mild angina as age advanced. Nevertheless, in over 1/3 of our cases followed for a year and a half or longer, their hearts were still stronger or better coordinated, or both, than they had been before operation, and there is reason to believe that the decline was leveling. At their last test, the great majority of patients still reported that subjective improvement in their angina continued. This subjective improvement was significantly related to objective improvement of cardiac contractility in our date, but there was one striking exception to this rule. Previous studies of results following internal mammary artery ligation anf the Vineberg procedure afford an important control to the present work.", "contents": "Early and late effects of the coronary bypass operation on cardiac contractility and coordination. In 100 cases of coronary heart disease, cardiac contractility was studied before and after coronary bypass by a flow method, the force ballistocardiogram, and by a pressure method, the carotid pulse derivative. The two methods gave very similar results in most cases, and the agreement of their averages was impressive. A marked increase in cardiac strength, reaching its maximum a month or two after operation, followed the procedure in most cases. Cardiac coordination was also improved at this time in many subjects, but complete cardiac normality was very rarely attained. This early postoperative improvement was greater in hearts judged to be weak before operation than in those judged to be normal in strength. The early cardiac improvement was seldom held in its entirety, but usually declined at a rate, which, if continued, would abolish the postoperative improvement in about three years. This rate of cardiac decline was many times faster than that found in healthy persons or in unoperated cases of mild angina as age advanced. Nevertheless, in over 1/3 of our cases followed for a year and a half or longer, their hearts were still stronger or better coordinated, or both, than they had been before operation, and there is reason to believe that the decline was leveling. At their last test, the great majority of patients still reported that subjective improvement in their angina continued. This subjective improvement was significantly related to objective improvement of cardiac contractility in our date, but there was one striking exception to this rule. Previous studies of results following internal mammary artery ligation anf the Vineberg procedure afford an important control to the present work."}
{"id": "PMID:1080349", "title": "Variant angina pectoris: Clinical and anatomic spectrum and results of coronary bypass surgery.", "content": "Twenty patients are described with the variant angina syndrome (recurrent angina at rest with S-T segment elevations occurring only during pain and no evolution of infarction). In contrast to patients previously reported on, all but one had progressive unstable angina before hospitalization. Angina was frequently associated with arrhythmias, including ventricular fibrillation (2 instances), ventricular tachycardia (4), frequent ventricular premature beats (5), atrioventricular block (4), sinus bradycardia (2), sinoatrial exit block (1) and supraventricular tachycardia (1). Seventeen patients had significant proximal stenosis of one or more coronary arteries with good distal vessels. Bypass surgery in 15 of these patients resulted in one noncardiac postoperative death, one perioperative infarction and relief of pain in all 14 survivors. After a 17 month mean follow-up period (range 4 to 38 months), all survivors are pain-free. Three patients had no significant coronary disease; one of these became asymptomatic with medical therapy, one continues to have angina and one died suddenly. Patients with normal coronary arteries could not be distinguished clinically or by electrocardiogram from those with severe obstructive lesions. This experience suggests that all patients with the variant angina syndrome should be studied by coronary angiography, and that most patients with significant fixed coronary lesions will do well after coronary bypass surgery.", "contents": "Variant angina pectoris: Clinical and anatomic spectrum and results of coronary bypass surgery. Twenty patients are described with the variant angina syndrome (recurrent angina at rest with S-T segment elevations occurring only during pain and no evolution of infarction). In contrast to patients previously reported on, all but one had progressive unstable angina before hospitalization. Angina was frequently associated with arrhythmias, including ventricular fibrillation (2 instances), ventricular tachycardia (4), frequent ventricular premature beats (5), atrioventricular block (4), sinus bradycardia (2), sinoatrial exit block (1) and supraventricular tachycardia (1). Seventeen patients had significant proximal stenosis of one or more coronary arteries with good distal vessels. Bypass surgery in 15 of these patients resulted in one noncardiac postoperative death, one perioperative infarction and relief of pain in all 14 survivors. After a 17 month mean follow-up period (range 4 to 38 months), all survivors are pain-free. Three patients had no significant coronary disease; one of these became asymptomatic with medical therapy, one continues to have angina and one died suddenly. Patients with normal coronary arteries could not be distinguished clinically or by electrocardiogram from those with severe obstructive lesions. This experience suggests that all patients with the variant angina syndrome should be studied by coronary angiography, and that most patients with significant fixed coronary lesions will do well after coronary bypass surgery."}
{"id": "PMID:1080350", "title": "Reappearance of anterior QRS forces after coronary bypass surgery. An electrovectorcardiographic study.", "content": "This report describes the reappearance of anterior QRS electrical forces in six patients after direct coronary arterial bypass surgery. Each patient had severe coronary artery disease including a segmental stenosis of the left anterior descending artery. Revascularization was performed by direct  anastomosis of the left mammary artery to the left anterior descending coronary artery and saphenous vein bypass of other stenotic coronary arteries. Preoperative electrocardiograms and vectorcardiograms showed patterns of anterior wall myocardial infarction with absent or diminutive anterior QRS forces. In each case, postoperative studies demonstrated the regeneration of anterior QRS forces within 10 days of operation. Although these patients represent a small percent of those with a preoperative pattern of infarction who undergo coronary revascularization, the findings demonstrate that electrically silent areas of myocardium may be altered and are not always synonymous with myocardial cell death. Chronic myocardial ischemia may in certain instances produce electrocardiographic and vectorcardiographic patterns of myocardial infarction that may be reversible upon reestablishment of perfusion to ischemic areas.", "contents": "Reappearance of anterior QRS forces after coronary bypass surgery. An electrovectorcardiographic study. This report describes the reappearance of anterior QRS electrical forces in six patients after direct coronary arterial bypass surgery. Each patient had severe coronary artery disease including a segmental stenosis of the left anterior descending artery. Revascularization was performed by direct  anastomosis of the left mammary artery to the left anterior descending coronary artery and saphenous vein bypass of other stenotic coronary arteries. Preoperative electrocardiograms and vectorcardiograms showed patterns of anterior wall myocardial infarction with absent or diminutive anterior QRS forces. In each case, postoperative studies demonstrated the regeneration of anterior QRS forces within 10 days of operation. Although these patients represent a small percent of those with a preoperative pattern of infarction who undergo coronary revascularization, the findings demonstrate that electrically silent areas of myocardium may be altered and are not always synonymous with myocardial cell death. Chronic myocardial ischemia may in certain instances produce electrocardiographic and vectorcardiographic patterns of myocardial infarction that may be reversible upon reestablishment of perfusion to ischemic areas."}
{"id": "PMID:1080351", "title": "Experimental coronary arterial occlusion and release. Effects on enzymes, electrocardiograms, myocardial contractility and reactive hyperemia.", "content": "After less than 1 hour of coronary arterial occlusion, the myocardium suffers irreversible changes as revealed by electron microscopy. Yet, the earliest clinical laboratory indexes of myocardial infarction--elevated serum enzyme levels and significant Q waves on the electrocardiogram--are not detected until at least 6 hours after coronary occlusion. To study the early period after coronary occlusion in the dog, occlusion of the left anterior descending coronary artery for 1 to 3 hours was followed by release, and coronary sinus and venous enzyme levels, the electrocardiogram and myocardial contractility from the infarcted area, and reactive hyperemia were monitored. Coronary sinus enzyme levels rose within 15 minutes after release of occlusion in half of the experiments with 1 to 1 1/2 hours and in all of those with 2 to 3 hours of occlusion, and this rise preceded the rise in venous levels by only 10 to 20 minutes. Significant Q waves appeared 15 to 30 minutes after release of occlusion as the serum enzymes were increasing. Thus, clinically, the delayed appearance of increased serum enzymes and significant electrocardiographic Q waves is probably largely due to a lack of circulation in the infarcted area rather than to prolonged survival time. Also, the venous enzyme level reflects the coronary sinus level minutes later. The presence of viable myocardium in the infarcted area was suggested by elevation of the S-T segment upon reclamping, and by residual myocardial contractility and retained capacity for reactive hyperemia. These findings occurred in some experiments even in the presence of a significant Q wave.", "contents": "Experimental coronary arterial occlusion and release. Effects on enzymes, electrocardiograms, myocardial contractility and reactive hyperemia. After less than 1 hour of coronary arterial occlusion, the myocardium suffers irreversible changes as revealed by electron microscopy. Yet, the earliest clinical laboratory indexes of myocardial infarction--elevated serum enzyme levels and significant Q waves on the electrocardiogram--are not detected until at least 6 hours after coronary occlusion. To study the early period after coronary occlusion in the dog, occlusion of the left anterior descending coronary artery for 1 to 3 hours was followed by release, and coronary sinus and venous enzyme levels, the electrocardiogram and myocardial contractility from the infarcted area, and reactive hyperemia were monitored. Coronary sinus enzyme levels rose within 15 minutes after release of occlusion in half of the experiments with 1 to 1 1/2 hours and in all of those with 2 to 3 hours of occlusion, and this rise preceded the rise in venous levels by only 10 to 20 minutes. Significant Q waves appeared 15 to 30 minutes after release of occlusion as the serum enzymes were increasing. Thus, clinically, the delayed appearance of increased serum enzymes and significant electrocardiographic Q waves is probably largely due to a lack of circulation in the infarcted area rather than to prolonged survival time. Also, the venous enzyme level reflects the coronary sinus level minutes later. The presence of viable myocardium in the infarcted area was suggested by elevation of the S-T segment upon reclamping, and by residual myocardial contractility and retained capacity for reactive hyperemia. These findings occurred in some experiments even in the presence of a significant Q wave."}
{"id": "PMID:1080352", "title": "Alterations in energy metabolism and ultrastructure upon reperfusion of the ischemic myocardium after coronary occlusion.", "content": "The effects on myocardial function, metabolism and ultrastructure of 60 minutes of reperfusion, instituted after 30, 60 and 90 minutes of occlusion of the left anterior descending coronary artery, were studied in 48 dogs. Twelve sham-operated dogs served as controls. Coronary occlusion for 60 or 90 minutes caused significant depression in the first derivative of left ventricular pressure (dP/dt) (P less than 0.05) that could not be reversed by reperfusion. Upon reperfusion, creatine phosphate stores in myocardium made ischemic for 30 and 60 minutes, but not for 90 minutes, returned toward control levels, but stores of adenosine triphosphate (ATP) and total nucleotides and the ATP/adenosine diphosphate ratio of myocardium subjected to 60 and 90 minutes of ischemia were further decreased. After 60 and 90 minutes of ischemia, swelling of the sarcoplasmic reticulum and mitochondrial damage (swelling, decreased matrix density and partial loss of cristae) were seen. Myofibrils were relaxed in all these groups. Reperfusion produced gross contraction of myofibrils and aggravated these changes in mitochondria and sarcoplasmic reticulum. In the hearts subjected to 90 minutes of ischemia these changes were gross. The levels of creatine phosphokinase, glutamic oxaloacetic transaminase and lactic dehydrogenase in the coronary sinus blood increased dramatically (P less than 0.05) upon reperfusion after 60 or 90 minutes of occlusion, indicating severe impairment of cell membranes. This secondary rise in serum enzyme activity during reperfusion should be taken into consideration when estimating the size of a myocardial infarct from enzyme changes alone. It appears that 60 and 90 minutes of ischemia cause severe myocardial damage that is not reversed by reperfusion maintained for 1 hour although longer periods of reperfusion may be beneficial.", "contents": "Alterations in energy metabolism and ultrastructure upon reperfusion of the ischemic myocardium after coronary occlusion. The effects on myocardial function, metabolism and ultrastructure of 60 minutes of reperfusion, instituted after 30, 60 and 90 minutes of occlusion of the left anterior descending coronary artery, were studied in 48 dogs. Twelve sham-operated dogs served as controls. Coronary occlusion for 60 or 90 minutes caused significant depression in the first derivative of left ventricular pressure (dP/dt) (P less than 0.05) that could not be reversed by reperfusion. Upon reperfusion, creatine phosphate stores in myocardium made ischemic for 30 and 60 minutes, but not for 90 minutes, returned toward control levels, but stores of adenosine triphosphate (ATP) and total nucleotides and the ATP/adenosine diphosphate ratio of myocardium subjected to 60 and 90 minutes of ischemia were further decreased. After 60 and 90 minutes of ischemia, swelling of the sarcoplasmic reticulum and mitochondrial damage (swelling, decreased matrix density and partial loss of cristae) were seen. Myofibrils were relaxed in all these groups. Reperfusion produced gross contraction of myofibrils and aggravated these changes in mitochondria and sarcoplasmic reticulum. In the hearts subjected to 90 minutes of ischemia these changes were gross. The levels of creatine phosphokinase, glutamic oxaloacetic transaminase and lactic dehydrogenase in the coronary sinus blood increased dramatically (P less than 0.05) upon reperfusion after 60 or 90 minutes of occlusion, indicating severe impairment of cell membranes. This secondary rise in serum enzyme activity during reperfusion should be taken into consideration when estimating the size of a myocardial infarct from enzyme changes alone. It appears that 60 and 90 minutes of ischemia cause severe myocardial damage that is not reversed by reperfusion maintained for 1 hour although longer periods of reperfusion may be beneficial."}
{"id": "PMID:1080353", "title": "The bleeding time as a test of hemostatic function.", "content": "The vascular component of hemostatis is determined by the bleeding time as designed by Duke or by the Ivy modification, which is a combined bleeding time-tourniquet test. A prolonged bleeding time is observed: (1) in thrombopathic thrombocytopenia, which may be (a) hereditary or (b) acquired, as in the immune types or from depression of bone marrow, and (2) athrombocytopenically, as in von Willebrand's disease, Glanzmann's thrombasthenia, or from aspirin intolerance. The role of a specific bleeding time determinant in the plasma (labile bleeding time factor of Perkins) is discussed and its possible relation to platelets suggested.", "contents": "The bleeding time as a test of hemostatic function. The vascular component of hemostatis is determined by the bleeding time as designed by Duke or by the Ivy modification, which is a combined bleeding time-tourniquet test. A prolonged bleeding time is observed: (1) in thrombopathic thrombocytopenia, which may be (a) hereditary or (b) acquired, as in the immune types or from depression of bone marrow, and (2) athrombocytopenically, as in von Willebrand's disease, Glanzmann's thrombasthenia, or from aspirin intolerance. The role of a specific bleeding time determinant in the plasma (labile bleeding time factor of Perkins) is discussed and its possible relation to platelets suggested."}
{"id": "PMID:1080354", "title": "Infectious arthritis in the neonate caused by Haemophilus influenzae.", "content": "Two newborns had hematogenous pyarthrosis due to Haemophilus influenzae. One infant had signs of sepsis and dactylitis involving several fingers and toes. She also developed a soft tissue abscess, meningitis, and a septic hip, and was found to be infected with a nontypable organism. In the second infant, a shoulder traumatized at birth became infected with a type b strain. In both cases, the patients were successfully treated, but delays occurred in selecting the optimal therapeutic agent because of failure to appreciate that Haemophilus may cause systemic infection in the newborn. In the first infant the source of the infection was identified as the mother's endocervical canal. This patient is also of interest because in contrast to previous reports of Haemophilus infection in the newborn, bactericidal activity was present in the maternal serum.", "contents": "Infectious arthritis in the neonate caused by Haemophilus influenzae. Two newborns had hematogenous pyarthrosis due to Haemophilus influenzae. One infant had signs of sepsis and dactylitis involving several fingers and toes. She also developed a soft tissue abscess, meningitis, and a septic hip, and was found to be infected with a nontypable organism. In the second infant, a shoulder traumatized at birth became infected with a type b strain. In both cases, the patients were successfully treated, but delays occurred in selecting the optimal therapeutic agent because of failure to appreciate that Haemophilus may cause systemic infection in the newborn. In the first infant the source of the infection was identified as the mother's endocervical canal. This patient is also of interest because in contrast to previous reports of Haemophilus infection in the newborn, bactericidal activity was present in the maternal serum."}
{"id": "PMID:1080355", "title": "Prothrombin complex concentrate: use in controlling the hemorrhagic diathesis of chronic liver disease.", "content": "A prothrombin complex concentrate was used in attempts to control life-threatening hemorrhage in 4 patients with chronic liver disease. The population manifested profuse bleeding from varices and/or hemorrhagic gastritis; 3 had Laennec's cirrhosis and 1 had postnecrotic cirrhosis from childhood hepatitis. In all patients the complex was given in amounts needed to raise the prothrombin (factor II) level to approximately 100% of normal. In all 4 cases the prothrombin time and prothrombin complex factors approached normal within 1-2 hr after beginning the infusion. In all patients bleeding ceased with correction of the clotting status. One patient rebled several hours after completing the infusion. In several patients, increases in factors V and VIII were noted following infusion of the concentrate. A further unexpected finding was a spontaneous increase in factors II and IX at 3 days postinfusion. Prothrombin complex concentrate appears to be useful in controlling the hemorrhage of chronic liver disease when used alone or in combination with other modalities to correct specific hemostatic defects; however, patients may be expected to rebleed when the effect of the concentrate wears off. Its use, therefore, should probably be restricted to those patients who are to undergo corrective surgery of the bleeding point once hemostasis is achieved.", "contents": "Prothrombin complex concentrate: use in controlling the hemorrhagic diathesis of chronic liver disease. A prothrombin complex concentrate was used in attempts to control life-threatening hemorrhage in 4 patients with chronic liver disease. The population manifested profuse bleeding from varices and/or hemorrhagic gastritis; 3 had Laennec's cirrhosis and 1 had postnecrotic cirrhosis from childhood hepatitis. In all patients the complex was given in amounts needed to raise the prothrombin (factor II) level to approximately 100% of normal. In all 4 cases the prothrombin time and prothrombin complex factors approached normal within 1-2 hr after beginning the infusion. In all patients bleeding ceased with correction of the clotting status. One patient rebled several hours after completing the infusion. In several patients, increases in factors V and VIII were noted following infusion of the concentrate. A further unexpected finding was a spontaneous increase in factors II and IX at 3 days postinfusion. Prothrombin complex concentrate appears to be useful in controlling the hemorrhage of chronic liver disease when used alone or in combination with other modalities to correct specific hemostatic defects; however, patients may be expected to rebleed when the effect of the concentrate wears off. Its use, therefore, should probably be restricted to those patients who are to undergo corrective surgery of the bleeding point once hemostasis is achieved."}
{"id": "PMID:1080357", "title": "The effect of menopause on serum cholesterol in American (Pima) Indian women.", "content": "Serum cholesterol levels in Pima Indian women were investigated to determine if menopause caused these levels to rise. The mean cholesterol values of 148 pre-menopausal women aged 34-54 when age-matched with 75 post-menopausal women were not significantly different. Sixty-five women passed through menopause while under observation and were examined sequentially for an average of 6.1 years. Their mean pre- and post-menopausal cholesterol levels of 182 +/- 5 mg/100 ml and 180 +/- 5 mg/100 ml were similar. Nor did menopause alter cholesterol levels in the subgroups of these patients with diabetes mellitus. Cholesterol levels in both Pima males and females showed no age-related rise, and were 20-30% lower than white populations. This study provides additional evidence against the postulated relationship between menopause and serum cholesterol.", "contents": "The effect of menopause on serum cholesterol in American (Pima) Indian women. Serum cholesterol levels in Pima Indian women were investigated to determine if menopause caused these levels to rise. The mean cholesterol values of 148 pre-menopausal women aged 34-54 when age-matched with 75 post-menopausal women were not significantly different. Sixty-five women passed through menopause while under observation and were examined sequentially for an average of 6.1 years. Their mean pre- and post-menopausal cholesterol levels of 182 +/- 5 mg/100 ml and 180 +/- 5 mg/100 ml were similar. Nor did menopause alter cholesterol levels in the subgroups of these patients with diabetes mellitus. Cholesterol levels in both Pima males and females showed no age-related rise, and were 20-30% lower than white populations. This study provides additional evidence against the postulated relationship between menopause and serum cholesterol."}
{"id": "PMID:1080358", "title": "Topical treatment of the burn patient.", "content": "The pathophysiology, classification and bacteriology of burns are discussed, as well as treatment of burns with silver nitrate, silver sulfadizine, silver allantoinate, mafenide acetate, furazolium chloride, povidone-iodine complex, antibiotics, antiproteolytics, fibrinolytics, Pseudomonas vaccine and antiglobulins.", "contents": "Topical treatment of the burn patient. The pathophysiology, classification and bacteriology of burns are discussed, as well as treatment of burns with silver nitrate, silver sulfadizine, silver allantoinate, mafenide acetate, furazolium chloride, povidone-iodine complex, antibiotics, antiproteolytics, fibrinolytics, Pseudomonas vaccine and antiglobulins."}
{"id": "PMID:1080359", "title": "Inhibitors of leukocyte chemotaxis in alcoholic liver disease.", "content": "Twenty-one of 42 patients (50 per cent) with alcoholic liver disease showed serum chemotactic inhibitory activity (CIA). CIA was not related to any single biochemical or histologic feature in the patients studied. The frequency of CIA was greatest in those with active infection. Serial studies demonstrated that CIA may be a transient phenomenon, associated with active alcoholic liver disease or appearance of infection. Nine of 15 patients showed skin test anergy; CIA was present in 8 of these 9 patients. Serum immunoglobulin A (IgA) and G (IgG) concentrations were significantly higher in patients with CIA when compared to those without CIA. Sucrose density gradient centrifugation of serums showing CIA yielded three peaks of inhibitory activity. Two had sedimentation coefficients of 10.7S and 6.8S, and the third was approximately 3S. The two higher molecular weight inhibitors were predominant in the 50 per cent ammonium sulfate precipitate. Immunoabsorption by anti-IgA but not by anti-IgG or IgM columns removed the ammonium sulfate precipitable chemotactic inhibitors. The appearance of chemotactic inhibitors in patients with alcoholic liver disease may have relevance to their apparent susceptibility to serious infections.", "contents": "Inhibitors of leukocyte chemotaxis in alcoholic liver disease. Twenty-one of 42 patients (50 per cent) with alcoholic liver disease showed serum chemotactic inhibitory activity (CIA). CIA was not related to any single biochemical or histologic feature in the patients studied. The frequency of CIA was greatest in those with active infection. Serial studies demonstrated that CIA may be a transient phenomenon, associated with active alcoholic liver disease or appearance of infection. Nine of 15 patients showed skin test anergy; CIA was present in 8 of these 9 patients. Serum immunoglobulin A (IgA) and G (IgG) concentrations were significantly higher in patients with CIA when compared to those without CIA. Sucrose density gradient centrifugation of serums showing CIA yielded three peaks of inhibitory activity. Two had sedimentation coefficients of 10.7S and 6.8S, and the third was approximately 3S. The two higher molecular weight inhibitors were predominant in the 50 per cent ammonium sulfate precipitate. Immunoabsorption by anti-IgA but not by anti-IgG or IgM columns removed the ammonium sulfate precipitable chemotactic inhibitors. The appearance of chemotactic inhibitors in patients with alcoholic liver disease may have relevance to their apparent susceptibility to serious infections."}
{"id": "PMID:1080360", "title": "Computer analysis of defined populations of lymphocytes irradiated in vitro. II. Analysis of thymus-dependent versus bone marrow-dependent cells.", "content": "Three uniform populations of T and B cells exposed to varying amounts of x-irradiation are examined utilizing computer-assisted morphometric analysis. These populations are: thoracic duct lymphocytes (TDL) from congenitally athymic (nude) mice (B cells); TDL from CBA mice treated with anti-Ig plus complement (T cells); and computer-selected untreated T cells from CBA TDL. Irradiated B cells show a more even dispersion of the nuclear chromatin and a dose-dependent increase in relative nuclear area beginning with the lowest dose evaluated (50 rads); no significan change in total optical density (OD) is demonstrable over the dose range evaluated (0 to 2000 rads). Anti-Ig-treated irradiated T cells demonstrate an initial shift toward lower OD values as a function of dose followed by a marked rise of OD values at 2000 rads, where numerous densely staining Feulgen-positive aggregates are identified. The relative nuclear area of this cell population also shows a biphasic response to radiation injury with an initial increase at the lower dose levels followed by a progressive decline to approximate control levels at 2000 rads. This effect is mirrored by the alteration in total OD which, after a decrease at low dose levels, approximates control values at 2000 rads. The computer-selected T cells show little change in OD values at the low-dose levels but show a marked increase in the more densely staining Feulgen-positive material following 2000 rads. This population reveals no apparent change in either relative nuclear area or total OD as a function of dose. Thus, untreated computer-selected T cells exhibit remarkably little evidence, morphologically, of radiation injury of doses associated with pronounced alterations on the part of B cells. In addition, treatment of a mixed cell population (CBA TDL) with anti-Ig plus complement to remove the B cells appears to alter the response of the residual T cells to radiation injury. These results, in conjunction with recent evidence to support the concept that T cells possess surface Ig, suggest that an Ig-anti-Ig interaction may alter the radiosensitivity of T cells.", "contents": "Computer analysis of defined populations of lymphocytes irradiated in vitro. II. Analysis of thymus-dependent versus bone marrow-dependent cells. Three uniform populations of T and B cells exposed to varying amounts of x-irradiation are examined utilizing computer-assisted morphometric analysis. These populations are: thoracic duct lymphocytes (TDL) from congenitally athymic (nude) mice (B cells); TDL from CBA mice treated with anti-Ig plus complement (T cells); and computer-selected untreated T cells from CBA TDL. Irradiated B cells show a more even dispersion of the nuclear chromatin and a dose-dependent increase in relative nuclear area beginning with the lowest dose evaluated (50 rads); no significan change in total optical density (OD) is demonstrable over the dose range evaluated (0 to 2000 rads). Anti-Ig-treated irradiated T cells demonstrate an initial shift toward lower OD values as a function of dose followed by a marked rise of OD values at 2000 rads, where numerous densely staining Feulgen-positive aggregates are identified. The relative nuclear area of this cell population also shows a biphasic response to radiation injury with an initial increase at the lower dose levels followed by a progressive decline to approximate control levels at 2000 rads. This effect is mirrored by the alteration in total OD which, after a decrease at low dose levels, approximates control values at 2000 rads. The computer-selected T cells show little change in OD values at the low-dose levels but show a marked increase in the more densely staining Feulgen-positive material following 2000 rads. This population reveals no apparent change in either relative nuclear area or total OD as a function of dose. Thus, untreated computer-selected T cells exhibit remarkably little evidence, morphologically, of radiation injury of doses associated with pronounced alterations on the part of B cells. In addition, treatment of a mixed cell population (CBA TDL) with anti-Ig plus complement to remove the B cells appears to alter the response of the residual T cells to radiation injury. These results, in conjunction with recent evidence to support the concept that T cells possess surface Ig, suggest that an Ig-anti-Ig interaction may alter the radiosensitivity of T cells."}
{"id": "PMID:1080361", "title": "The development of anti-immunoglobulin antibodies in rhesus monkeys repeatedly exposed to Schistosoma japonicum.", "content": "The development of anti-immunoglobulin (Ig) antibodies in rhesus monkeys repeatedly exposed to Schistosoma japonicum cercariae was studied. Anti-Ig developed in all 8 monkeys exposed 5 times to cercariae of the Formosan strain, while none of 4 monkeys exposed once to the Philippine strain developed such antibodies in the same period. All monkeys developing anti-Ig had specificities for IgA, 6 of 8 for IgM and IgG, and 7 of 8 for rabbit Ig. The persistence of anti-Ig was greatly extended in the monkeys exposed initially to the Formosan strain and then challenged with the Philippine strain. A single monkey exposed once to  the Philippine strain developed anti-IgA and anti-rabbit Ig 85 weeks postinfection. No relationship between host reaction to trapped eggs and the development of anti-Ig was discerned. The results suggest that immunization protocols designed for humans be carefully examined for their potential immunopathological side effects.", "contents": "The development of anti-immunoglobulin antibodies in rhesus monkeys repeatedly exposed to Schistosoma japonicum. The development of anti-immunoglobulin (Ig) antibodies in rhesus monkeys repeatedly exposed to Schistosoma japonicum cercariae was studied. Anti-Ig developed in all 8 monkeys exposed 5 times to cercariae of the Formosan strain, while none of 4 monkeys exposed once to the Philippine strain developed such antibodies in the same period. All monkeys developing anti-Ig had specificities for IgA, 6 of 8 for IgM and IgG, and 7 of 8 for rabbit Ig. The persistence of anti-Ig was greatly extended in the monkeys exposed initially to the Formosan strain and then challenged with the Philippine strain. A single monkey exposed once to  the Philippine strain developed anti-IgA and anti-rabbit Ig 85 weeks postinfection. No relationship between host reaction to trapped eggs and the development of anti-Ig was discerned. The results suggest that immunization protocols designed for humans be carefully examined for their potential immunopathological side effects."}
{"id": "PMID:1080362", "title": "Role of splenectomy in Felty's syndrome.", "content": "To evaluate the effectiveness of splenectomy in the treatment of Felty's syndrome (association of rheumatoid arthritis, leukopenia, and splenomegaly), such experience from 1968 to 1972 at the University of Alabama Medical Center in Birmingham was analyzed. There were five patients with Felty's syndrome who underwent splenectomy. In all five patients, there was no operative morbidity, no blood transfusions were required, and leukopenia and susceptibility to infection were greatly improved; neutropenia disappeared in all but one patient. Splenectomy appears to benefit most patients with Felty's syndrome.", "contents": "Role of splenectomy in Felty's syndrome. To evaluate the effectiveness of splenectomy in the treatment of Felty's syndrome (association of rheumatoid arthritis, leukopenia, and splenomegaly), such experience from 1968 to 1972 at the University of Alabama Medical Center in Birmingham was analyzed. There were five patients with Felty's syndrome who underwent splenectomy. In all five patients, there was no operative morbidity, no blood transfusions were required, and leukopenia and susceptibility to infection were greatly improved; neutropenia disappeared in all but one patient. Splenectomy appears to benefit most patients with Felty's syndrome."}
{"id": "PMID:1080363", "title": "Technic of chronic thoracic duct \"side fistula\" for immunologic evaluation of patients with cancer.", "content": "A technic suitable for long-term study of the human thoracic duct circulation under physiological conditions is described. The results and observations obtained in ten patients studied for three to twenty-one days are presented.", "contents": "Technic of chronic thoracic duct \"side fistula\" for immunologic evaluation of patients with cancer. A technic suitable for long-term study of the human thoracic duct circulation under physiological conditions is described. The results and observations obtained in ten patients studied for three to twenty-one days are presented."}
{"id": "PMID:1080364", "title": "Unusual manifestations of pancreatic pseudocysts and their surgical management.", "content": "Six cases representing selected complications of pancreatic pseudocyst are reported and their surgical management is discussed. Patients with mediastinal extension of a pseudocyst frequently present with symptoms in the chest rather than in the abdomen. Chronic recurrent pleural effusion, rich in protein and amylase, often accompanies mediastinal extension of a pancreatic pseudocyst. It is important to recognize that such an effusion almost certainly represents disruption of the pancreatic duct with formation of a pancreatic pseudocyst or a pancreaticopleural fistula. Internal drainage from below the diaphragm is the treatment of choice for pancreatic pseudocysts extending into the mediastinum. To be certain that obstructive jaundice is due to a pancreatic pseudocyst, there must be operative demonstration of compression of the common bile duct by the pseudocyst, relief of the obstruction by surgical drainage of the cyst, and subsequent disappearance of jaundice. Cysts that cause jaundice are located in the head of the pancreas, and cystoduodenostomy is the treatment of choice. Intraperitoneal rupture has been associated with a high mortality, but with adequate fluid replacement, prompt evacuation of the cyst contents from the peritoneal cavity, and adequate drainage, mortality can be lowered. Pancreatic ascites is much more common than is generally supposed and may result from a leaking pancreatic pseudocyst. In contrast to cirrhotic ascites, pancreatic ascites produces elevation of both the serum amylase level and protein concentration. Massive hemorrhage from pancreatic pseudocysts is usually due to the development of a false aneurysm in a branch of the celiac axis in the wall of the pseudocyst, with subsequent rupture of the aneurysm into the gut or peritoneal cavity. Any patient with a pancreatic pseudocyst who shows signs of bleeding should have prompt arteriography for determination of the bleeding site and appropriate surgical control. Pancreaticobronchial fistula is a rare complication. Treatment should be directed toward adequate drainage of the pseudocyst in the abdomen.", "contents": "Unusual manifestations of pancreatic pseudocysts and their surgical management. Six cases representing selected complications of pancreatic pseudocyst are reported and their surgical management is discussed. Patients with mediastinal extension of a pseudocyst frequently present with symptoms in the chest rather than in the abdomen. Chronic recurrent pleural effusion, rich in protein and amylase, often accompanies mediastinal extension of a pancreatic pseudocyst. It is important to recognize that such an effusion almost certainly represents disruption of the pancreatic duct with formation of a pancreatic pseudocyst or a pancreaticopleural fistula. Internal drainage from below the diaphragm is the treatment of choice for pancreatic pseudocysts extending into the mediastinum. To be certain that obstructive jaundice is due to a pancreatic pseudocyst, there must be operative demonstration of compression of the common bile duct by the pseudocyst, relief of the obstruction by surgical drainage of the cyst, and subsequent disappearance of jaundice. Cysts that cause jaundice are located in the head of the pancreas, and cystoduodenostomy is the treatment of choice. Intraperitoneal rupture has been associated with a high mortality, but with adequate fluid replacement, prompt evacuation of the cyst contents from the peritoneal cavity, and adequate drainage, mortality can be lowered. Pancreatic ascites is much more common than is generally supposed and may result from a leaking pancreatic pseudocyst. In contrast to cirrhotic ascites, pancreatic ascites produces elevation of both the serum amylase level and protein concentration. Massive hemorrhage from pancreatic pseudocysts is usually due to the development of a false aneurysm in a branch of the celiac axis in the wall of the pseudocyst, with subsequent rupture of the aneurysm into the gut or peritoneal cavity. Any patient with a pancreatic pseudocyst who shows signs of bleeding should have prompt arteriography for determination of the bleeding site and appropriate surgical control. Pancreaticobronchial fistula is a rare complication. Treatment should be directed toward adequate drainage of the pseudocyst in the abdomen."}
{"id": "PMID:1080365", "title": "Heparinless left heart bypass for resection of thoracic aortic aneurysms.", "content": "Our clinical experience employing heparinless left heart bypass for the resection of twenty-three thoracic aneurysms is presented. In our current technic of heparinless left heart bypass, plastic tubing coated with nonthrombogenic polyurethane-polyvinyl-graphite material and a conventional roller pump are employed. The reduced size of the aneurysm below the aortic clamp during bypass facilitates careful dissection of the aneurysm and the aorta. Twenty-two of twenty-three patients underwent successful thoracic aneurysmectomy with this technic. None had subsequent paraplegia and the postoperative blood loss was minimal. Heparinless left heart bypass is a simple and safe procedure to facilitate thoracic aneurysm resection.", "contents": "Heparinless left heart bypass for resection of thoracic aortic aneurysms. Our clinical experience employing heparinless left heart bypass for the resection of twenty-three thoracic aneurysms is presented. In our current technic of heparinless left heart bypass, plastic tubing coated with nonthrombogenic polyurethane-polyvinyl-graphite material and a conventional roller pump are employed. The reduced size of the aneurysm below the aortic clamp during bypass facilitates careful dissection of the aneurysm and the aorta. Twenty-two of twenty-three patients underwent successful thoracic aneurysmectomy with this technic. None had subsequent paraplegia and the postoperative blood loss was minimal. Heparinless left heart bypass is a simple and safe procedure to facilitate thoracic aneurysm resection."}
{"id": "PMID:1080367", "title": "[The course of zonular fibers in the area of pars plicata corporis cilaris pars plana, ora serrata and retina. Eectron microsocpic study].", "content": "The course of zonular fibers in the area of pars plicata corporis ciliaris could be shown down to the pigmented epithelial layer and its basement membrane. S ingle fibrils enter in a connection with the plasma membrane in allinvestigated parts. Fibrils could  be observed in the cytoplasm of the ora serrata. For the other areas (pars plicata corporis ciliaris, parsplana) this can be suggested too.", "contents": "[The course of zonular fibers in the area of pars plicata corporis cilaris pars plana, ora serrata and retina. Eectron microsocpic study]. The course of zonular fibers in the area of pars plicata corporis ciliaris could be shown down to the pigmented epithelial layer and its basement membrane. S ingle fibrils enter in a connection with the plasma membrane in allinvestigated parts. Fibrils could  be observed in the cytoplasm of the ora serrata. For the other areas (pars plicata corporis ciliaris, parsplana) this can be suggested too."}
{"id": "PMID:1080369", "title": "The clinical picture of Eales' disease.", "content": "The clinical findings in 316 eyes with Eales' disease and 41 eyes true idiopathic periphlebitis were analyzed. Eales' disease differed clearly from idiopathic periphlebitis in a predominance of male patients, a marked tendency toward bilateral disease in males, the aspect of vascular sheathing and the absence of inflammatory signs from the vitreous body. The clinical picture of Eales' disease was characterized by avascular areas in the retinal periphery, followed posteriorly by microaneurysms, rope-ladder-like dilations of capillary channels, tortousity of neighbouring vessels and spontaneous choriortinal scars. The more pronounced finds were neovascularizations (84%) hemorrhages (58%), obliterated vessels (39%) and vascular sheathing (34%). In contrast to idiopathic periphlebitis, Eales' disease is considered a primary, non-inflammatory disorder of the walls of the peripheral retinal vessels, namely the shunt vessels.", "contents": "The clinical picture of Eales' disease. The clinical findings in 316 eyes with Eales' disease and 41 eyes true idiopathic periphlebitis were analyzed. Eales' disease differed clearly from idiopathic periphlebitis in a predominance of male patients, a marked tendency toward bilateral disease in males, the aspect of vascular sheathing and the absence of inflammatory signs from the vitreous body. The clinical picture of Eales' disease was characterized by avascular areas in the retinal periphery, followed posteriorly by microaneurysms, rope-ladder-like dilations of capillary channels, tortousity of neighbouring vessels and spontaneous choriortinal scars. The more pronounced finds were neovascularizations (84%) hemorrhages (58%), obliterated vessels (39%) and vascular sheathing (34%). In contrast to idiopathic periphlebitis, Eales' disease is considered a primary, non-inflammatory disorder of the walls of the peripheral retinal vessels, namely the shunt vessels."}
{"id": "PMID:1080370", "title": "Ultrastructure of the microvessels of the iris in mammals with special reference to their permeability.", "content": "The ultrastructure of the microvessels of the guinea pig, rat, rabbit and cat was studied in an effort to discover the morphological basis of the permeability of the iris vessels. The microvessels of the iris revealed a non-fenestrated endothelium. A relatively wide intercellular space and macula occludens were seen at the intercellular junctions of the endothelial cells in the capillaries and postcapillary venules. It is concluded that these intercellular spaces may form the small pore system which makes the diffusion through the iris vessels possible. Transcapillary exchange of high molecular substances is made possible by the vesicular transport system of the micropinocytosis vesicles.", "contents": "Ultrastructure of the microvessels of the iris in mammals with special reference to their permeability. The ultrastructure of the microvessels of the guinea pig, rat, rabbit and cat was studied in an effort to discover the morphological basis of the permeability of the iris vessels. The microvessels of the iris revealed a non-fenestrated endothelium. A relatively wide intercellular space and macula occludens were seen at the intercellular junctions of the endothelial cells in the capillaries and postcapillary venules. It is concluded that these intercellular spaces may form the small pore system which makes the diffusion through the iris vessels possible. Transcapillary exchange of high molecular substances is made possible by the vesicular transport system of the micropinocytosis vesicles."}
{"id": "PMID:1080371", "title": "[Fixation disparity in peripheral and central binocular vision under conditions of prismatic heterophoria (author's transl)].", "content": "A critical review of the literature shows that there are controversal interpretations of thephenomenon fixation disparity. The former findings of Ogle and coworkers are ascertained byinvertigator to close the central gap in the fusional patter. Formerly the gap was essentialdue to polarisation techiques. 1. Without central gap fixation disparity decreases, but remainssignificantly different from zero. 2. Diminution or closing of the gap leads to more preciseresults, since the position of the eyes is better controlled. 3. Straining the binocular vision with high prism power cortical tolerances increase when fusion was aided by central stimuli. Fixation disparity could be extended up to 20' (minutes of are) which is three times the well accepted size of the Panum area.", "contents": "[Fixation disparity in peripheral and central binocular vision under conditions of prismatic heterophoria (author's transl)]. A critical review of the literature shows that there are controversal interpretations of thephenomenon fixation disparity. The former findings of Ogle and coworkers are ascertained byinvertigator to close the central gap in the fusional patter. Formerly the gap was essentialdue to polarisation techiques. 1. Without central gap fixation disparity decreases, but remainssignificantly different from zero. 2. Diminution or closing of the gap leads to more preciseresults, since the position of the eyes is better controlled. 3. Straining the binocular vision with high prism power cortical tolerances increase when fusion was aided by central stimuli. Fixation disparity could be extended up to 20' (minutes of are) which is three times the well accepted size of the Panum area."}
{"id": "PMID:1080372", "title": "[Experimental Research on the O2 supply of the eye author's transl].", "content": "To investigate the O2 supply conditions of the corneal endothelium and the lens by the aqueous hymor, O2 solubility coefficients of aqueous humor as well as in the lens had to be determined. The current assumption that the vitreous body is a possible O2 pool had to be examined. The following conclusions are drawn from the measured values of Bunsel O2 solubility coefficients (ml O2ml atm) of bovine aqueous humor, lens homogenates, vitreous bodys as well as of solutions of hyaluronic acid at temperatures from 10 to 40 degrees C: 1. The physiological rate of oxygen transport by the aqueous suffices to supply the corneal endothelium and the lens with oxygen, the temperature gradients within the anterior chamber favour the O2 supply of the corneal endothelium compared to other ocular tissues. 2. The O2 content of the aqueous can maintain O2 consumption of corneal endothelium for about 20 min theoretically or for about 5 min the lens ones. 3. The vitreous body can act as a possibel O2 pool only with regard to the O2 supply of the lens. In this case the pool can maintain O2 consumption of the shole lens for about 20 min. 4. During physiological growth of the lens the central part expands, which is characterized by an O2 concentration near zero. Therefore, the O2 supply of the lens is maintained for only less than one minute by the content of the lens itself.", "contents": "[Experimental Research on the O2 supply of the eye author's transl]. To investigate the O2 supply conditions of the corneal endothelium and the lens by the aqueous hymor, O2 solubility coefficients of aqueous humor as well as in the lens had to be determined. The current assumption that the vitreous body is a possible O2 pool had to be examined. The following conclusions are drawn from the measured values of Bunsel O2 solubility coefficients (ml O2ml atm) of bovine aqueous humor, lens homogenates, vitreous bodys as well as of solutions of hyaluronic acid at temperatures from 10 to 40 degrees C: 1. The physiological rate of oxygen transport by the aqueous suffices to supply the corneal endothelium and the lens with oxygen, the temperature gradients within the anterior chamber favour the O2 supply of the corneal endothelium compared to other ocular tissues. 2. The O2 content of the aqueous can maintain O2 consumption of corneal endothelium for about 20 min theoretically or for about 5 min the lens ones. 3. The vitreous body can act as a possibel O2 pool only with regard to the O2 supply of the lens. In this case the pool can maintain O2 consumption of the shole lens for about 20 min. 4. During physiological growth of the lens the central part expands, which is characterized by an O2 concentration near zero. Therefore, the O2 supply of the lens is maintained for only less than one minute by the content of the lens itself."}
{"id": "PMID:1080373", "title": "Optically induced eye torsion. I. Fusion cyclovergence.", "content": "Fusional cyclovergence was demonstrated for the first time, using ab objective photographic method. It was discovered that the results of subjective and objective measurements of cyclovergence agreed exactly. The largest fusional cyclovergence observed was 8 degrees. The cyclovergence partly cancels out the cyclodisparity presented. The greater part of the cyclodisparity usually remains. The maximum aplitude of the sensory cyclofusion is of the order of 8 degrees. The cyclovergence response is greatest when large fusion images with numerous horizontal contours are presented. The motor mechanism of cyclofusion presupposes the existence of recently demonstrated cyclodisparity detectors. The amplitude of sensory fusion is determined by the vertical diameter of the peripheral Panum areas.", "contents": "Optically induced eye torsion. I. Fusion cyclovergence. Fusional cyclovergence was demonstrated for the first time, using ab objective photographic method. It was discovered that the results of subjective and objective measurements of cyclovergence agreed exactly. The largest fusional cyclovergence observed was 8 degrees. The cyclovergence partly cancels out the cyclodisparity presented. The greater part of the cyclodisparity usually remains. The maximum aplitude of the sensory cyclofusion is of the order of 8 degrees. The cyclovergence response is greatest when large fusion images with numerous horizontal contours are presented. The motor mechanism of cyclofusion presupposes the existence of recently demonstrated cyclodisparity detectors. The amplitude of sensory fusion is determined by the vertical diameter of the peripheral Panum areas."}
{"id": "PMID:1080374", "title": "Coat's disease. An epidemiologic and Fluorescein angiographic study.", "content": "Coats' disease represents a disorder of the retinal vascular walls. In a series of 112 consecutive eyes with Coats' disease the involvement was unilateral in 90.2%. The majority (72%) of patients were male. 57.8% were under 20 years of age. There was, however, a second peak of the disease (30.4%) between the age of 41 and 60. Severe cases were found mainly among juvenile patients, whereas in the senile group milder cases prevailed. Fluorescein angiographic findings comprised vascular aneurysms in almost all cases, rarefaction and coarsening of the capillary bed in 71%, capillary occlusions in 70%, occlusions of arterioles or arteries in 27%, shunt formations in 27%, extensive widening of arteries or veins in 27% and leakage of dye mainly in advanced cases. The participation of the central retina in the disease process was variable. Evidence is presented that Leber's military aneurysm retinitis is not a separate entity but a special form of Coats' disease.", "contents": "Coat's disease. An epidemiologic and Fluorescein angiographic study. Coats' disease represents a disorder of the retinal vascular walls. In a series of 112 consecutive eyes with Coats' disease the involvement was unilateral in 90.2%. The majority (72%) of patients were male. 57.8% were under 20 years of age. There was, however, a second peak of the disease (30.4%) between the age of 41 and 60. Severe cases were found mainly among juvenile patients, whereas in the senile group milder cases prevailed. Fluorescein angiographic findings comprised vascular aneurysms in almost all cases, rarefaction and coarsening of the capillary bed in 71%, capillary occlusions in 70%, occlusions of arterioles or arteries in 27%, shunt formations in 27%, extensive widening of arteries or veins in 27% and leakage of dye mainly in advanced cases. The participation of the central retina in the disease process was variable. Evidence is presented that Leber's military aneurysm retinitis is not a separate entity but a special form of Coats' disease."}
{"id": "PMID:1080375", "title": "[The effect of human leukocyte interteron (HIF) on experimental viral keratitis in monkeys (author's transl)].", "content": "12 african green monkeys were inoculated in both eyes with herpes simplex virus typ 1 and 16 rhesus monkeys with vaccinia virus. The right eyes were treated with human leukocyte interferon (HIF) while the left eyes served as controls and showed the typical keratitis, 7 out of 8 herpes eyes and all 9 vaccinia eyes which were treated prophylactically or simultaneiously with HIF showed no signs of disease. When HIF was given later in the course of the infection the keratitis was either little influenced or not modified at all. The reasons are discussed.", "contents": "[The effect of human leukocyte interteron (HIF) on experimental viral keratitis in monkeys (author's transl)]. 12 african green monkeys were inoculated in both eyes with herpes simplex virus typ 1 and 16 rhesus monkeys with vaccinia virus. The right eyes were treated with human leukocyte interferon (HIF) while the left eyes served as controls and showed the typical keratitis, 7 out of 8 herpes eyes and all 9 vaccinia eyes which were treated prophylactically or simultaneiously with HIF showed no signs of disease. When HIF was given later in the course of the infection the keratitis was either little influenced or not modified at all. The reasons are discussed."}
{"id": "PMID:1080376", "title": "[On scanning electron microscpy to the anterior part of the eye with special regard to the coruse of zonula Zinnii (author's transl)].", "content": "Three areas in which zonular fibers of Zinn enter were demonstrated with the aid of scanning electron microscopy. 1. The area, which applied to the edge of the lens. 2. The area of Pars plicata corporis ciliaris. 3. The area of Pars plana. The connection of zonuar fibers with limited membran of vitreous body was presented too.", "contents": "[On scanning electron microscpy to the anterior part of the eye with special regard to the coruse of zonula Zinnii (author's transl)]. Three areas in which zonular fibers of Zinn enter were demonstrated with the aid of scanning electron microscopy. 1. The area, which applied to the edge of the lens. 2. The area of Pars plicata corporis ciliaris. 3. The area of Pars plana. The connection of zonuar fibers with limited membran of vitreous body was presented too."}
{"id": "PMID:1080377", "title": "[Electronmicroscopic investigation about the course of zonular fiber in the area of ora serrata and the peripheral retina (author's transl)].", "content": "Single zonular fibers could be shown in the area of ora serrata and the peripheral parts of retina between the basement membrane of pigmented epithelium and the elastic tissue of Bruchsmembran. The occurrence of fibroblasts in the proximity of zonular fibers was noticeable.", "contents": "[Electronmicroscopic investigation about the course of zonular fiber in the area of ora serrata and the peripheral retina (author's transl)]. Single zonular fibers could be shown in the area of ora serrata and the peripheral parts of retina between the basement membrane of pigmented epithelium and the elastic tissue of Bruchsmembran. The occurrence of fibroblasts in the proximity of zonular fibers was noticeable."}
{"id": "PMID:1080379", "title": "Alpha-antitrypsin Pi types in postmortem blood.", "content": "It is possible to determine alpha1-antitrypsin Pi types from serum obtained at necropsy.  The Pi types were identical in 37 paired antemortem and postmortem samples.  Blood transfusion in the 72 hours preceeding death may produce serum that cannot be typed.  The frequency of the Pis allele was high in this study (0.074) and may reflect terminal alterations in alpha1-antitrypsin mobility and thus Pi typing, or a higher frequency in the population studied.  The Pis allele was particularly frequent among Canadians with French names and Canadians born in Italy, Greece, and Portugal.  The prevalence and severity of emphysema were not increased in PiA and Pis heterozygotes, but the groups studied were small and the variable, smoking, could not be adequately controlled.  Studies of larger groups are recommended.", "contents": "Alpha-antitrypsin Pi types in postmortem blood. It is possible to determine alpha1-antitrypsin Pi types from serum obtained at necropsy.  The Pi types were identical in 37 paired antemortem and postmortem samples.  Blood transfusion in the 72 hours preceeding death may produce serum that cannot be typed.  The frequency of the Pis allele was high in this study (0.074) and may reflect terminal alterations in alpha1-antitrypsin mobility and thus Pi typing, or a higher frequency in the population studied.  The Pis allele was particularly frequent among Canadians with French names and Canadians born in Italy, Greece, and Portugal.  The prevalence and severity of emphysema were not increased in PiA and Pis heterozygotes, but the groups studied were small and the variable, smoking, could not be adequately controlled.  Studies of larger groups are recommended."}
{"id": "PMID:1080382", "title": "Effect of indomethacin and phenylbutazone-prednisone combination of gastric mucosa: gastroscopic and histological study.", "content": "The effect of a phenylbutazone-prednisone combination and indomethacin on the anatomical state of the gastric mucosa were studied by gastroscopy, gastrophotography, and gastrobiopsy. All four patients examined had redness of the antral mucosa after administration of either drug. Intramucosal gastric haemorrhage was present in two patients after both phenylbutazone-prednisone combination and indomethacin. Mucosal erosions occurred in three cases after the phenylbutazone-prednisone combination, one of them slight, and in two cases after indomethacin. On histological examination, increased intramucosal haemorrhage could be seen in the specimens from all four patients after indomethacin, and in the specimens from three patients after the phenylbutazone-prednisone combination. There was considerable mucosal vasodilatation in one patient on the phenylbutazone-prednisone combination and in two patients on indomethacin. The effects of both drugs on the gastric mucosa were similar. None of the subjects suffered from abdominal symptoms during the drug administration.", "contents": "Effect of indomethacin and phenylbutazone-prednisone combination of gastric mucosa: gastroscopic and histological study. The effect of a phenylbutazone-prednisone combination and indomethacin on the anatomical state of the gastric mucosa were studied by gastroscopy, gastrophotography, and gastrobiopsy. All four patients examined had redness of the antral mucosa after administration of either drug. Intramucosal gastric haemorrhage was present in two patients after both phenylbutazone-prednisone combination and indomethacin. Mucosal erosions occurred in three cases after the phenylbutazone-prednisone combination, one of them slight, and in two cases after indomethacin. On histological examination, increased intramucosal haemorrhage could be seen in the specimens from all four patients after indomethacin, and in the specimens from three patients after the phenylbutazone-prednisone combination. There was considerable mucosal vasodilatation in one patient on the phenylbutazone-prednisone combination and in two patients on indomethacin. The effects of both drugs on the gastric mucosa were similar. None of the subjects suffered from abdominal symptoms during the drug administration."}
{"id": "PMID:1080380", "title": "Temporal bone histopathological findings in trisomy 13 syndrome.", "content": "This study reports the histopathological findings of 14 temporal bones from infants with trisomy 13 syndrome. The most primitive anomalies in the structures of the inner and middle ears in the present series are those of the semicircular canals, particularly of the horizontal canals: flattened horizontal canal cristae, absence or opening of the utricular endolymphatic valve, small facial nerve, and obtuse angle of the geniculate area of the facial nerve. Each ear demonstrated more than one of those anomalies. The anomalies present features similar to those found in the structures of the normal six to ten-week fetus. Many other mild anomalies observed appear to demonstrate features similar to those seen in the same structures in later fetal life. Reviewing these findings, most of the anomalies that were found in the inner and middle ears appear to be the result of poor development of the structures for reasons which are now unclear. In addition, middle ear infection was found in all cases.", "contents": "Temporal bone histopathological findings in trisomy 13 syndrome. This study reports the histopathological findings of 14 temporal bones from infants with trisomy 13 syndrome. The most primitive anomalies in the structures of the inner and middle ears in the present series are those of the semicircular canals, particularly of the horizontal canals: flattened horizontal canal cristae, absence or opening of the utricular endolymphatic valve, small facial nerve, and obtuse angle of the geniculate area of the facial nerve. Each ear demonstrated more than one of those anomalies. The anomalies present features similar to those found in the structures of the normal six to ten-week fetus. Many other mild anomalies observed appear to demonstrate features similar to those seen in the same structures in later fetal life. Reviewing these findings, most of the anomalies that were found in the inner and middle ears appear to be the result of poor development of the structures for reasons which are now unclear. In addition, middle ear infection was found in all cases."}
{"id": "PMID:1080383", "title": "Some characteristics of Anaerovibrio lipolytica a rumen lipolytic organism.", "content": "Strains of Anaerovibrio lipolytica isolated from sheep- and cow-rumen contents on a linseed oil -- rumen fluid -- agar medium fermented ribose, glycerol and DL-lactate. Fermentation products from glycerol were propionate and succinate, while ribose, fructose and DL-lactate were fermented mainly to acetate, propionate and carbon dioxide. Propionate is formed in this organism by the dicarboxylic acid pathway similarly as in propionibacteria. Measurements of the rate of lipolysis by pure cultures suggest that the organism may play an important role in the lipolytic activity of rumen contents of sheep. The demonstrated fact that the cell-free lipase excreted in the culture medium can easily be adsorbed on particulate matter in autoclaved rumen fluid may explain the absence of free lipase in clarified rumen liquor.", "contents": "Some characteristics of Anaerovibrio lipolytica a rumen lipolytic organism. Strains of Anaerovibrio lipolytica isolated from sheep- and cow-rumen contents on a linseed oil -- rumen fluid -- agar medium fermented ribose, glycerol and DL-lactate. Fermentation products from glycerol were propionate and succinate, while ribose, fructose and DL-lactate were fermented mainly to acetate, propionate and carbon dioxide. Propionate is formed in this organism by the dicarboxylic acid pathway similarly as in propionibacteria. Measurements of the rate of lipolysis by pure cultures suggest that the organism may play an important role in the lipolytic activity of rumen contents of sheep. The demonstrated fact that the cell-free lipase excreted in the culture medium can easily be adsorbed on particulate matter in autoclaved rumen fluid may explain the absence of free lipase in clarified rumen liquor."}
{"id": "PMID:1080384", "title": "Isolation and characterization of some new oxalate-decomposing bacteria.", "content": "Forty-one cultures degrading and assimilating oxalate were isolated from chicken dung. Characterizarion indicated six different types. One of these belonged to thhe genus Alcaligenes hitherto never reported to degrade oxalte. Three groups of Pseudomonas strains differed physiologically from strains already known.", "contents": "Isolation and characterization of some new oxalate-decomposing bacteria. Forty-one cultures degrading and assimilating oxalate were isolated from chicken dung. Characterizarion indicated six different types. One of these belonged to thhe genus Alcaligenes hitherto never reported to degrade oxalte. Three groups of Pseudomonas strains differed physiologically from strains already known."}
{"id": "PMID:1080385", "title": "Hansenula lynferdii sp.nov.", "content": "A single strain of an undescribed Hansenula species was recovered from soil. A description of the new species is given and its possible relationship to the other species of the genus is discussed.", "contents": "Hansenula lynferdii sp.nov. A single strain of an undescribed Hansenula species was recovered from soil. A description of the new species is given and its possible relationship to the other species of the genus is discussed."}
{"id": "PMID:1080386", "title": "Ultrastructural aspects of wall regeneration by Pythium protoplasts.", "content": "Electron microscope studies were made of wall regeneration by Pythium protoplasts. Wall regeneration began with the formation of a loose network of fibrils on the surface of the protoplast followed by increase in density of the fibrillar mesh and deposition of granular mitrix material. The majority of the protoplasts did not develop beyond the loose fibrillar network stage, however a small percentage were able to complete wall formation and to form hyphal tubes. A clear zone of demarcation was  visible between the fibrillar surface of the protoplast and the smooth surface at the base of the developing hyphal tube.", "contents": "Ultrastructural aspects of wall regeneration by Pythium protoplasts. Electron microscope studies were made of wall regeneration by Pythium protoplasts. Wall regeneration began with the formation of a loose network of fibrils on the surface of the protoplast followed by increase in density of the fibrillar mesh and deposition of granular mitrix material. The majority of the protoplasts did not develop beyond the loose fibrillar network stage, however a small percentage were able to complete wall formation and to form hyphal tubes. A clear zone of demarcation was  visible between the fibrillar surface of the protoplast and the smooth surface at the base of the developing hyphal tube."}
{"id": "PMID:1080381", "title": "Paget's disease of the temporal bone.", "content": "Osteitis deformans Paget is a fairly common, heritable, sometimes progressive disease of bone which affects primarily the axial skeleton and may lead to deformity and weakness. It affects 3% of the population over forty years of age and males more frequently than females. The skull and temporal bones become involved in about two-thirds of the patients. Progressive involvement of the temporal bones may lead to alteration of position, increase in size and change of architecture of the petrous pyramid, external canal, middle ear and inner ear capsule. These changes in turn may produce impairment of hearing (about 30-50% of cases) and vestibular function (20-25%). The clinical, radiological, and pathological manifestations of Paget's disease of the temporal bone are discussed in detail and explained with photomicrographs. Examples of tumor formations and vascular changes are presented.", "contents": "Paget's disease of the temporal bone. Osteitis deformans Paget is a fairly common, heritable, sometimes progressive disease of bone which affects primarily the axial skeleton and may lead to deformity and weakness. It affects 3% of the population over forty years of age and males more frequently than females. The skull and temporal bones become involved in about two-thirds of the patients. Progressive involvement of the temporal bones may lead to alteration of position, increase in size and change of architecture of the petrous pyramid, external canal, middle ear and inner ear capsule. These changes in turn may produce impairment of hearing (about 30-50% of cases) and vestibular function (20-25%). The clinical, radiological, and pathological manifestations of Paget's disease of the temporal bone are discussed in detail and explained with photomicrographs. Examples of tumor formations and vascular changes are presented."}
{"id": "PMID:1080387", "title": "Polysaccharide production and the possible occurrence of GDP-D-mannose dehydrogenase in Azotobacter vinelandii.", "content": "During the growth of Azotobacter vinelandii in batch culture in Burk's 2% glucose medium supplemented with 50 mg EDTA per litre, water-insoluble capsular polysaccaride material accumulated in cultures prior to the appearance of water-soluble polysaccharide in the culture medium. On isolation, hydrolysis and chromatography, both these polysaccharides were observed to be composed of carbohydrate monomers having the same chromatographic mobilities as glucose, rhamnos, guluronic acid and mannuronic acid. The activity of GDP-D-mannose dehydrogenase recorded in crude cell-free extracts from Azotobacter vinelandii, when these polysaccharides were produced, may indicate a close similarity between the biosynthetic pathway of alginate synthesis in marine Phaeophyceae and this soil microorganism.", "contents": "Polysaccharide production and the possible occurrence of GDP-D-mannose dehydrogenase in Azotobacter vinelandii. During the growth of Azotobacter vinelandii in batch culture in Burk's 2% glucose medium supplemented with 50 mg EDTA per litre, water-insoluble capsular polysaccaride material accumulated in cultures prior to the appearance of water-soluble polysaccharide in the culture medium. On isolation, hydrolysis and chromatography, both these polysaccharides were observed to be composed of carbohydrate monomers having the same chromatographic mobilities as glucose, rhamnos, guluronic acid and mannuronic acid. The activity of GDP-D-mannose dehydrogenase recorded in crude cell-free extracts from Azotobacter vinelandii, when these polysaccharides were produced, may indicate a close similarity between the biosynthetic pathway of alginate synthesis in marine Phaeophyceae and this soil microorganism."}
{"id": "PMID:1080388", "title": "Scanning isoelectric focusing of cholera enterotoxin in polyacrylamide gels.", "content": "Scanning isoelectric focusing has been employed for continuous monitoring of the isoelectric spectrum of highly purified cholera enterotoxin in 4% polyacrylamide gels containing 2% ampholytes pH 3-10. The resolution obtained by this technique is of high order because at no instance during focusing interruption of current occurs and thus diffusion of the isolated protein moieties is suppressed. An added aspect of scanning isoelectric focusing was that it allowed estimation of the minimal focusing time of cholera enterotoxin. Thus under the standard assay procedure, the main basic component of cholera enterotoxin was focused in 5800 sec, while the other at least 3 minor acidic and anodic components were focused in approximately 19000 sec. Focusing of cholera enterotoxin in the presence of 6 mu urea allowed the visualization of 5 well defined and about equal components. The proteinaceous nature of the observed peaks was verified by scanning at wavelenghts other than 280 nm, staining of gels for protein, and varying the concentration of the enterotoxin. The design of scanning isoelectric focusing equipment is presented. Reproducibility, economy of sample, and ampholytes and simplicity of experimental technique were some of the features of this apparatus. The resolution of scanning isoelectric focusing was found to be superior to that of ordinary disc and SDS gel electrophoresis.", "contents": "Scanning isoelectric focusing of cholera enterotoxin in polyacrylamide gels. Scanning isoelectric focusing has been employed for continuous monitoring of the isoelectric spectrum of highly purified cholera enterotoxin in 4% polyacrylamide gels containing 2% ampholytes pH 3-10. The resolution obtained by this technique is of high order because at no instance during focusing interruption of current occurs and thus diffusion of the isolated protein moieties is suppressed. An added aspect of scanning isoelectric focusing was that it allowed estimation of the minimal focusing time of cholera enterotoxin. Thus under the standard assay procedure, the main basic component of cholera enterotoxin was focused in 5800 sec, while the other at least 3 minor acidic and anodic components were focused in approximately 19000 sec. Focusing of cholera enterotoxin in the presence of 6 mu urea allowed the visualization of 5 well defined and about equal components. The proteinaceous nature of the observed peaks was verified by scanning at wavelenghts other than 280 nm, staining of gels for protein, and varying the concentration of the enterotoxin. The design of scanning isoelectric focusing equipment is presented. Reproducibility, economy of sample, and ampholytes and simplicity of experimental technique were some of the features of this apparatus. The resolution of scanning isoelectric focusing was found to be superior to that of ordinary disc and SDS gel electrophoresis."}
{"id": "PMID:1080389", "title": "DNA base composition of species of the genus Saccharomyces.", "content": "DNA base compositions (GC content) of Saccharomyces species are reported and discussed. Several amendments of the four groups given by van der Walt are suggested, viz. the transfer of S. kluyveri to group 1, and of S. eupagycus, S. cidri, S. montanus, S. microellipsodes and S. florentinus to group 2. The synonomy of S. amurcae and S. cidri is suggested. The DNA base compositions revealed two possible pairs of sibling species: S. elegans and S. bailii, with a difference in GC content of 4.1%; S. dairensis and S. servazzii with a difference in GC content of ca. 3%. S. mrakii had a GC content of 47.3-48.5% the highest encountered in this genus and similar to that of Kluyveromyces thermotolerans.", "contents": "DNA base composition of species of the genus Saccharomyces. DNA base compositions (GC content) of Saccharomyces species are reported and discussed. Several amendments of the four groups given by van der Walt are suggested, viz. the transfer of S. kluyveri to group 1, and of S. eupagycus, S. cidri, S. montanus, S. microellipsodes and S. florentinus to group 2. The synonomy of S. amurcae and S. cidri is suggested. The DNA base compositions revealed two possible pairs of sibling species: S. elegans and S. bailii, with a difference in GC content of 4.1%; S. dairensis and S. servazzii with a difference in GC content of ca. 3%. S. mrakii had a GC content of 47.3-48.5% the highest encountered in this genus and similar to that of Kluyveromyces thermotolerans."}
{"id": "PMID:1080390", "title": "Bacteriocin typing of Pseudomonas putrefaciens from food, human clinical specimens, and other sources.", "content": "With the use of 4 bacteriocin donor strains of Pseudomonas putrefaciens most low G+C strins were readily distinguished from high G+C strains. Two bacteriocin donor strains exhibited autoinhibition when subjected to bacteriocin typing.", "contents": "Bacteriocin typing of Pseudomonas putrefaciens from food, human clinical specimens, and other sources. With the use of 4 bacteriocin donor strains of Pseudomonas putrefaciens most low G+C strins were readily distinguished from high G+C strains. Two bacteriocin donor strains exhibited autoinhibition when subjected to bacteriocin typing."}
{"id": "PMID:1080391", "title": "Substrate inhibition in Pseudomonas oxalaticus OX1:  a kinetic study of growth inhibition by oxalate and formate using extended cultures.", "content": "Pseudomonas oxalaticus OX1 has been grown in a mineral salts medium with oxalate or formate as the sole source of carbon and energy. At concentrations of these substrates above 50 mM inhibition of growth was indicated by a long and variable lag phase in batch culture. This inhibition was further studied by estimating maximum specific growth rates at different substrate concentrations using the extended culture technique for control of the substrate concentration. With formate, inhibition became apparent at substrate concentrations above 20 mM, whereas oxalate inhibited growth at concentrations above 15 mM. Complete inhibition was not observed even at concentrations of 100 mM. A number of inhibition functions were fitted with the experimental data using computer analysis. The results indicated that the Haldane equation was the simplest function to describe quantitatively the kinetics of the observed substrate inhibition. Studies on the rate of oxygen uptake at different concentrations of oxalate indicated that respiration was much more sensitive to inhibition than growth. However with formate, inhibition of respiration was not observed up to concentrations of 50 mM, indicating that different mechanisms may underlie the observed growth inhibition by the two substrates.", "contents": "Substrate inhibition in Pseudomonas oxalaticus OX1:  a kinetic study of growth inhibition by oxalate and formate using extended cultures. Pseudomonas oxalaticus OX1 has been grown in a mineral salts medium with oxalate or formate as the sole source of carbon and energy. At concentrations of these substrates above 50 mM inhibition of growth was indicated by a long and variable lag phase in batch culture. This inhibition was further studied by estimating maximum specific growth rates at different substrate concentrations using the extended culture technique for control of the substrate concentration. With formate, inhibition became apparent at substrate concentrations above 20 mM, whereas oxalate inhibited growth at concentrations above 15 mM. Complete inhibition was not observed even at concentrations of 100 mM. A number of inhibition functions were fitted with the experimental data using computer analysis. The results indicated that the Haldane equation was the simplest function to describe quantitatively the kinetics of the observed substrate inhibition. Studies on the rate of oxygen uptake at different concentrations of oxalate indicated that respiration was much more sensitive to inhibition than growth. However with formate, inhibition of respiration was not observed up to concentrations of 50 mM, indicating that different mechanisms may underlie the observed growth inhibition by the two substrates."}
{"id": "PMID:1080392", "title": "L-Phase variants of Agromyces ramosus.", "content": "Agromyces ramosus, which is a numerically prevalent bacterium in soil, was easily induced into the L-phase by growing it on agar media containing low levels of penicillin or glycine. The L-forms were stable after initial contact with the inducing agent and could not be reverted to the bacterial form by any of the procedures tried. These results are discussed in relation to a possible natural occurrence of L-forms of this bacterium in soil.", "contents": "L-Phase variants of Agromyces ramosus. Agromyces ramosus, which is a numerically prevalent bacterium in soil, was easily induced into the L-phase by growing it on agar media containing low levels of penicillin or glycine. The L-forms were stable after initial contact with the inducing agent and could not be reverted to the bacterial form by any of the procedures tried. These results are discussed in relation to a possible natural occurrence of L-forms of this bacterium in soil."}
{"id": "PMID:1080393", "title": "Pichia philogaea sp. nov.", "content": "Two strains of an undescribed Pichia species were recovered from soil. A description of the new species is given and its possible relationship with the other species of the genus is discussed.", "contents": "Pichia philogaea sp. nov. Two strains of an undescribed Pichia species were recovered from soil. A description of the new species is given and its possible relationship with the other species of the genus is discussed."}
{"id": "PMID:1080394", "title": "Two undescribed hyphomycetes from Malaysia.", "content": "Raffaelea variabilis is described as a new species in culture from Lannea grandis. It is distinguished by turbinate to cuneiform conidia measuring 4-16 times 2.5-7.5 mum, and compared and contrasted with established species. Trichocladium lobatum is described as a new species in culture with 1-2 euseptate spherical conidia, 19-22 mum diam., ornamented with flabelliform, spathulate or petaloid lobes 7 mum long. It is compared and contrasted with established Trichocladium species and representatives of Chlamydomyces, Histoplasma, Mycogone, Sepedonium and Thermomyces.", "contents": "Two undescribed hyphomycetes from Malaysia. Raffaelea variabilis is described as a new species in culture from Lannea grandis. It is distinguished by turbinate to cuneiform conidia measuring 4-16 times 2.5-7.5 mum, and compared and contrasted with established species. Trichocladium lobatum is described as a new species in culture with 1-2 euseptate spherical conidia, 19-22 mum diam., ornamented with flabelliform, spathulate or petaloid lobes 7 mum long. It is compared and contrasted with established Trichocladium species and representatives of Chlamydomyces, Histoplasma, Mycogone, Sepedonium and Thermomyces."}
{"id": "PMID:1080395", "title": "Lipomyces anomalus sp. nov.", "content": "A description of a new yeast species from soil - Lipomyces anomalus - is given. It differs from all the accepted Lipomyces spp. by the formation of abundant pseudomycelium, absence of capsules, the assimilation properties, and its habitat: the north taiga subzone.", "contents": "Lipomyces anomalus sp. nov. A description of a new yeast species from soil - Lipomyces anomalus - is given. It differs from all the accepted Lipomyces spp. by the formation of abundant pseudomycelium, absence of capsules, the assimilation properties, and its habitat: the north taiga subzone."}
{"id": "PMID:1080396", "title": "Two new species of the genus Sterigmatomyces.", "content": "The descriptions of two new species of the genus Sterigmatomyces, St. nectairii and St. penicillatus are given. The diagnosis of the genus is extended to starch production, positive or absent. An earlier described species, St. acheniorum Buhagiar et Barnett, is transferred to the genus Rhodotorula.", "contents": "Two new species of the genus Sterigmatomyces. The descriptions of two new species of the genus Sterigmatomyces, St. nectairii and St. penicillatus are given. The diagnosis of the genus is extended to starch production, positive or absent. An earlier described species, St. acheniorum Buhagiar et Barnett, is transferred to the genus Rhodotorula."}
{"id": "PMID:1080397", "title": "Three new asporogenous yeasts found in industrial waste water.", "content": "Representatives of three new asporogenous species, Candida flavificans, Candida inositophila, and Torulopsis sorbophila were isolated from washings of ion-exchange resin in a guanosine monophosphate manufacturing plant. The DNA base composition, proton-magnetic-resonance spectrum of extracted polysaccharides, and serological characteristics of the yeasts were investigated in addition to the characteristics commonly employed. Descriptions of the new species are given.", "contents": "Three new asporogenous yeasts found in industrial waste water. Representatives of three new asporogenous species, Candida flavificans, Candida inositophila, and Torulopsis sorbophila were isolated from washings of ion-exchange resin in a guanosine monophosphate manufacturing plant. The DNA base composition, proton-magnetic-resonance spectrum of extracted polysaccharides, and serological characteristics of the yeasts were investigated in addition to the characteristics commonly employed. Descriptions of the new species are given."}
{"id": "PMID:1080402", "title": "Smoking and disease: effect on serum antitrypsin in hospitalized patients.", "content": "The mean serum antitrypsin (AT) activity for 1,829 patients hospitalized with medical problems exceeded the mean for a group of blood bank donors by 51%. Analysis of data from individual patients in terms of the general category (there were 16) of their disease and smoking status (smoker, nonsmoker, former smoker) indicated significant differences due to disease status and smoking status. The highest mean AT levels were associated with infectious, respiratory, and neoplastic diseases. Smokers had significantly higher mean levels than nonsmokers, and lung cancer patients had significantly higher mean levels than those with other malignant neoplasms. Among smokers (as a group) mean AT levels. through elevated relative to nonsmokers were not significantly related to duration or intensity of smoking; but former smokers showed a decline (with time following cessation of smoking) in their mean AT level to the mean level for nonsmokers. These findings provide further evidence of the sensitivity of serum antitrypsin activity to environmental influences.", "contents": "Smoking and disease: effect on serum antitrypsin in hospitalized patients. The mean serum antitrypsin (AT) activity for 1,829 patients hospitalized with medical problems exceeded the mean for a group of blood bank donors by 51%. Analysis of data from individual patients in terms of the general category (there were 16) of their disease and smoking status (smoker, nonsmoker, former smoker) indicated significant differences due to disease status and smoking status. The highest mean AT levels were associated with infectious, respiratory, and neoplastic diseases. Smokers had significantly higher mean levels than nonsmokers, and lung cancer patients had significantly higher mean levels than those with other malignant neoplasms. Among smokers (as a group) mean AT levels. through elevated relative to nonsmokers were not significantly related to duration or intensity of smoking; but former smokers showed a decline (with time following cessation of smoking) in their mean AT level to the mean level for nonsmokers. These findings provide further evidence of the sensitivity of serum antitrypsin activity to environmental influences."}
{"id": "PMID:1080403", "title": "Proteinase inhibitors in rheumatoid arthritis.", "content": "The concentrations of five normally occurring protease inhibitors in serum and synovial fluid were compared in patients with rheumatoid arthritis, osteoarthrosis, and normal controls. The patients with rheumatoid arthritis showed a significant rise in alpha1-antitrypsin, alpha1-antichymotrypsin, and inter-alpha-trypsin inhibitor (in decreasing order) in serum as well as in synovial fluid. In synovial fluid the inhibitors were present in their native form and bound to hyaluronate. A large molecular protein with immunological specificity of alpha1-antitrypsin, presumably a complex of alpha1-antitrypsin and a protease, could be shown in synovial fluid of all patients with classical and probable rheumatoid arthritis and not in that of the other subjects studied.23Author", "contents": "Proteinase inhibitors in rheumatoid arthritis. The concentrations of five normally occurring protease inhibitors in serum and synovial fluid were compared in patients with rheumatoid arthritis, osteoarthrosis, and normal controls. The patients with rheumatoid arthritis showed a significant rise in alpha1-antitrypsin, alpha1-antichymotrypsin, and inter-alpha-trypsin inhibitor (in decreasing order) in serum as well as in synovial fluid. In synovial fluid the inhibitors were present in their native form and bound to hyaluronate. A large molecular protein with immunological specificity of alpha1-antitrypsin, presumably a complex of alpha1-antitrypsin and a protease, could be shown in synovial fluid of all patients with classical and probable rheumatoid arthritis and not in that of the other subjects studied.23Author"}
{"id": "PMID:1080404", "title": "Cross-sectional studies on Schistosoma mansoni infection in northeast Brazil.", "content": "Studies on the prevalence and morbidity of Schistosoma mansoni infection were carried out in selected areas of northeastern Brazil. The study revealed that both prevalence and morbidity rates were significantly higher in rural than in urban areas. No statistically significant differences in prevalence and morbidity were found in relation to sex. The age-specific prevalence curve was found to be of the usual shape found in highly endemic areas of the country: it reaches its peak in the 10-14 group, and slightly decreases in the older groups. Although it is generally admitted that hepatosplenic forms of the disease occur more frequently between 10 and 40 years of age, the present study has shown that the proportion of these severe cases increases with increase of age.", "contents": "Cross-sectional studies on Schistosoma mansoni infection in northeast Brazil. Studies on the prevalence and morbidity of Schistosoma mansoni infection were carried out in selected areas of northeastern Brazil. The study revealed that both prevalence and morbidity rates were significantly higher in rural than in urban areas. No statistically significant differences in prevalence and morbidity were found in relation to sex. The age-specific prevalence curve was found to be of the usual shape found in highly endemic areas of the country: it reaches its peak in the 10-14 group, and slightly decreases in the older groups. Although it is generally admitted that hepatosplenic forms of the disease occur more frequently between 10 and 40 years of age, the present study has shown that the proportion of these severe cases increases with increase of age."}
{"id": "PMID:1080407", "title": "Mesencephalic porjections of the rectus lateralis muscle afferents in the cat.", "content": "Responses to passive stretch applied to the rectus lateralis muscle (RL) were recorded with a microelectrode from the mesencephalic nucleus of the fifth nerve (Mes V) in cat enc\u00e9phale isol\u00e9 preparation. The necessary conditions to attribute the unit response to an eye muscle stretch are the followings: i) respond neither to jaw movements nor to pressure applied to the eye ball: ii) be activated with a short latency, following stretch of the eye muscles; iii) be excited by a light touch applied to a small area of one eye with a glass rod. The characteristics of the Mes V responses following stretch of the eye muscle are similar to those obtained in a primary afferent fibre from a skeletal muscle. Horseradish peroxidase was injected in the RL muscle of young and adult cats. As a consequence of fast retrograde axonal transport, the tracer was found to accumulate in the corresponding motoneurones of the VI nucleus. In addition, the enzymes accumulation was also found in cells of Mes V corresponding to the somata of RL sensory terminals. Units responding to RL stretchs, and primary sensory neurons labelled by the tracer, were found in the same area of Mes V.", "contents": "Mesencephalic porjections of the rectus lateralis muscle afferents in the cat. Responses to passive stretch applied to the rectus lateralis muscle (RL) were recorded with a microelectrode from the mesencephalic nucleus of the fifth nerve (Mes V) in cat enc\u00e9phale isol\u00e9 preparation. The necessary conditions to attribute the unit response to an eye muscle stretch are the followings: i) respond neither to jaw movements nor to pressure applied to the eye ball: ii) be activated with a short latency, following stretch of the eye muscles; iii) be excited by a light touch applied to a small area of one eye with a glass rod. The characteristics of the Mes V responses following stretch of the eye muscle are similar to those obtained in a primary afferent fibre from a skeletal muscle. Horseradish peroxidase was injected in the RL muscle of young and adult cats. As a consequence of fast retrograde axonal transport, the tracer was found to accumulate in the corresponding motoneurones of the VI nucleus. In addition, the enzymes accumulation was also found in cells of Mes V corresponding to the somata of RL sensory terminals. Units responding to RL stretchs, and primary sensory neurons labelled by the tracer, were found in the same area of Mes V."}
{"id": "PMID:1080408", "title": "The problem-oriented record. Problem definition.", "content": "A problem is defined when the clinician judges a dysfunction is apparent or highly probable for the patient in one or more of three areas: physiological, psychological, or social functioning. In defining specific problems, data assessments are often confounded with the data themselves. To \"lump\" or \"split\" problems in psychiatry should be by theoretical and practical considerations. Problem indicators should be \"split\" when the clinician believes they are not different indicators of the same problem or treatments for the problem indicators will be different. They can be \"lumped\" when the clinician believes they are different indicators of the same problem, or when the treatment will be the same. Criteria supporting the belief of a single problem construct are met when indicators co-vary, are functionally equivalent, or respond similarly to the same treatment variable.", "contents": "The problem-oriented record. Problem definition. A problem is defined when the clinician judges a dysfunction is apparent or highly probable for the patient in one or more of three areas: physiological, psychological, or social functioning. In defining specific problems, data assessments are often confounded with the data themselves. To \"lump\" or \"split\" problems in psychiatry should be by theoretical and practical considerations. Problem indicators should be \"split\" when the clinician believes they are not different indicators of the same problem or treatments for the problem indicators will be different. They can be \"lumped\" when the clinician believes they are different indicators of the same problem, or when the treatment will be the same. Criteria supporting the belief of a single problem construct are met when indicators co-vary, are functionally equivalent, or respond similarly to the same treatment variable."}
{"id": "PMID:1080410", "title": "Current surgical management of left ventricular aneurysm.", "content": "One hundred thirty-two patients with ventricular aneurysm had cardiac catheterization, coronary anglography, and ventriculography. More than 50 percent of the patients, in addition to aneurysmectomy, had other procedures consisting of revascularization, mitral valve replacement, and closure of ventricular septal performation. The overall mortality was 10 percent. Aneurysmectomy alone had a higher mortality (9 percent) when compared with combined resection of the aneurysm and coronary bypass (5.4 percent). During a six-month to 4-1/2-year follow-up (mean, 21 months), seven patients died, representing a late mortality of 6 percent. This study confirms the importance of complete cardiac evaluation in patients with ventricular aneurysm and shows that, in spite of extensive combined operative procedures required in more than 50 percent of the patients, the results are favorable.", "contents": "Current surgical management of left ventricular aneurysm. One hundred thirty-two patients with ventricular aneurysm had cardiac catheterization, coronary anglography, and ventriculography. More than 50 percent of the patients, in addition to aneurysmectomy, had other procedures consisting of revascularization, mitral valve replacement, and closure of ventricular septal performation. The overall mortality was 10 percent. Aneurysmectomy alone had a higher mortality (9 percent) when compared with combined resection of the aneurysm and coronary bypass (5.4 percent). During a six-month to 4-1/2-year follow-up (mean, 21 months), seven patients died, representing a late mortality of 6 percent. This study confirms the importance of complete cardiac evaluation in patients with ventricular aneurysm and shows that, in spite of extensive combined operative procedures required in more than 50 percent of the patients, the results are favorable."}
{"id": "PMID:1080411", "title": "Electrocoagulation of bleeding in the upper part of the gastrointestinal tract: a preliminary experimental clinical report.", "content": "Electrocoagulation of gastric or esophageal bleeding sites was evaluated in dogs using a flexible suction coagulator electrode passed through the endoscope using an electrocautery unit (Cameron-Miller model 80-7910). Acute and chronic coagulation studies in 25 dogs showed that electrocoagulation of both the esophageal and gastric mucosa was safe at an electrocautery unit setting of 5 for three to five seconds. This level of coagulation was successful in stopping bleeding created from actively bleeding esophageal and gastric mucosal defects by taking multiple large biopsy specimens. Based on these data, six patients with active bleeding gastric lesions have undergone mucosal coagulation. This successfully stopped the bleeding initially in all six patients; two patients rebled and required operative intervention within 48 hours. Further clinical evaluation is being implemented to determine its clinical role in the treatment of bleeding in the upper part of the gastrointestinal tract.", "contents": "Electrocoagulation of bleeding in the upper part of the gastrointestinal tract: a preliminary experimental clinical report. Electrocoagulation of gastric or esophageal bleeding sites was evaluated in dogs using a flexible suction coagulator electrode passed through the endoscope using an electrocautery unit (Cameron-Miller model 80-7910). Acute and chronic coagulation studies in 25 dogs showed that electrocoagulation of both the esophageal and gastric mucosa was safe at an electrocautery unit setting of 5 for three to five seconds. This level of coagulation was successful in stopping bleeding created from actively bleeding esophageal and gastric mucosal defects by taking multiple large biopsy specimens. Based on these data, six patients with active bleeding gastric lesions have undergone mucosal coagulation. This successfully stopped the bleeding initially in all six patients; two patients rebled and required operative intervention within 48 hours. Further clinical evaluation is being implemented to determine its clinical role in the treatment of bleeding in the upper part of the gastrointestinal tract."}
{"id": "PMID:1080409", "title": "[Recent views on the functional histological classification of malignant lymphomas].", "content": "The recent immunological classification of malignant lymphoma (ML) proposed by Lukes and Collins is examined. It is based on morphological changes linked to the dynamics of the reactive or secondary follicular clear centres. B-cells (follicular centre) are small or large and may cleft or not; they may proceed to macrophage or plasma cell types. T cells (around the follicles) are small and round, and have a convolute nucleus. ML types are distinguished by the predominance of one cell element, or by a mixture of cells from the maturative line of the follicular centre, i.e. T-cell (or convolute) lymphomas and B-cell (or follicular centre) lymphomas. Stem cells have none of these features. The classification was applied in a series of 32 non-Hodgkin and 24 Hodgkin ML's. Of the former, 23 were classed as B-cell types: 8 small and 5 large cleft cells; 7 small and 3 large non-cleft cells. The remainder were: 2 B-cell immunoblastic sarcomas, 3 histiocyte ML, 2 small lymphocyte ML, 1 ML with convolute lymphocytes, and 1 case with indeterminate cells (\"stem cell lymphoma\"). The 24 Hodgkin cases were classified in accordance with Rye's pattern (based on the work of Lukes et al.). It was noted that the numerical prevalence of convolute cells gradually fell, passing from lymphocyte dominance to lymphocyte depletion. At the same time, there was an increase in follicular centre cells and a diminution of plasma cell tending lymphocytes. The problems raised by this new immunological and morphogical classification of ML are discussed.", "contents": "[Recent views on the functional histological classification of malignant lymphomas]. The recent immunological classification of malignant lymphoma (ML) proposed by Lukes and Collins is examined. It is based on morphological changes linked to the dynamics of the reactive or secondary follicular clear centres. B-cells (follicular centre) are small or large and may cleft or not; they may proceed to macrophage or plasma cell types. T cells (around the follicles) are small and round, and have a convolute nucleus. ML types are distinguished by the predominance of one cell element, or by a mixture of cells from the maturative line of the follicular centre, i.e. T-cell (or convolute) lymphomas and B-cell (or follicular centre) lymphomas. Stem cells have none of these features. The classification was applied in a series of 32 non-Hodgkin and 24 Hodgkin ML's. Of the former, 23 were classed as B-cell types: 8 small and 5 large cleft cells; 7 small and 3 large non-cleft cells. The remainder were: 2 B-cell immunoblastic sarcomas, 3 histiocyte ML, 2 small lymphocyte ML, 1 ML with convolute lymphocytes, and 1 case with indeterminate cells (\"stem cell lymphoma\"). The 24 Hodgkin cases were classified in accordance with Rye's pattern (based on the work of Lukes et al.). It was noted that the numerical prevalence of convolute cells gradually fell, passing from lymphocyte dominance to lymphocyte depletion. At the same time, there was an increase in follicular centre cells and a diminution of plasma cell tending lymphocytes. The problems raised by this new immunological and morphogical classification of ML are discussed."}
{"id": "PMID:1080412", "title": "General surgery problems in patients with spinal cord injuries.", "content": "Twenty-four patients with spinal cord injuries were studied to correlate their responses to intra-abdominal disease with the level and completeness of the cord lesion. Patients with complete cervical lesions and lesions of the upper part of the thoracic region (C-4 to T-6) usually responded by early noniocalized abdominal pain associated with signs of autonomic dysreflexia. As the disease progressed to involve the parietal peritoneum, these patients were more capable of localizing pain to the corresponding dermatome, whereas patients with incomplete lesions were able to localize their pain earlier. Patients with lumbar lesions and lesions of the lower part of the thoracic region (T-7 to L-3) were able to localize their pain earlier than those with lesions located higher in the thoracic region. All patients had delayed diagnoses except those with hemorrhage of the upper part of the gastrointestinal tract. Irrespective of level of cord lesion, increased pulse rate was themost prominent objective acute intra-abdominal pathologic finding. Shoulder pain in the quadriplegic is a most helpful sign.", "contents": "General surgery problems in patients with spinal cord injuries. Twenty-four patients with spinal cord injuries were studied to correlate their responses to intra-abdominal disease with the level and completeness of the cord lesion. Patients with complete cervical lesions and lesions of the upper part of the thoracic region (C-4 to T-6) usually responded by early noniocalized abdominal pain associated with signs of autonomic dysreflexia. As the disease progressed to involve the parietal peritoneum, these patients were more capable of localizing pain to the corresponding dermatome, whereas patients with incomplete lesions were able to localize their pain earlier. Patients with lumbar lesions and lesions of the lower part of the thoracic region (T-7 to L-3) were able to localize their pain earlier than those with lesions located higher in the thoracic region. All patients had delayed diagnoses except those with hemorrhage of the upper part of the gastrointestinal tract. Irrespective of level of cord lesion, increased pulse rate was themost prominent objective acute intra-abdominal pathologic finding. Shoulder pain in the quadriplegic is a most helpful sign."}
{"id": "PMID:1080413", "title": "Diagnostic sonography in general surgery.", "content": "Eighty-seven patients who had sonographic examinations at the Orange County Medical Center were studied retrospectively to evaluate the usefulness and reliability of this noninvasive technique, and sonographic results were compared with findings at operation or findings made using other diagnostic methods. With a variety of lesions, the overall accuracy of diagnosis by sonography was 85%. In the remaining 15% of studies, false-positive or false-negative results were observed. The accuracy varied with the organ involved, the greatest being renal and hepatic masses. Ultrasound, when used in addition to careful surgical assessment of the patient, was found to be most useful in the diagnosis and follow-up of abdominal problems in surgical patients.", "contents": "Diagnostic sonography in general surgery. Eighty-seven patients who had sonographic examinations at the Orange County Medical Center were studied retrospectively to evaluate the usefulness and reliability of this noninvasive technique, and sonographic results were compared with findings at operation or findings made using other diagnostic methods. With a variety of lesions, the overall accuracy of diagnosis by sonography was 85%. In the remaining 15% of studies, false-positive or false-negative results were observed. The accuracy varied with the organ involved, the greatest being renal and hepatic masses. Ultrasound, when used in addition to careful surgical assessment of the patient, was found to be most useful in the diagnosis and follow-up of abdominal problems in surgical patients."}
{"id": "PMID:1080414", "title": "[Morphology of the synaptic apparatus of the motor neurons of the frog's spinal cord].", "content": "The method of light microscopy was used in order to study the synapses of motor neurons of the thoracic and lumbar parts of the spinal cord of intact frogs. Under study were the shape of the synapses, their size, as well as the quantitative distribution on the bodies and dendrites of neurons. It was established that the distribution density of active synaptic terminations on the motor neurons of the lumbar part was higher than the density of active synapses in the thoracic part, and the amount of synaptic terminations on dendrites was greater than on the neuron bodies; the snyaptic terminations of different shape were different in size; the occurrence of certain shapes of synaptic terminations in the thoracic and lumbar parts was almost the same.", "contents": "[Morphology of the synaptic apparatus of the motor neurons of the frog's spinal cord]. The method of light microscopy was used in order to study the synapses of motor neurons of the thoracic and lumbar parts of the spinal cord of intact frogs. Under study were the shape of the synapses, their size, as well as the quantitative distribution on the bodies and dendrites of neurons. It was established that the distribution density of active synaptic terminations on the motor neurons of the lumbar part was higher than the density of active synapses in the thoracic part, and the amount of synaptic terminations on dendrites was greater than on the neuron bodies; the snyaptic terminations of different shape were different in size; the occurrence of certain shapes of synaptic terminations in the thoracic and lumbar parts was almost the same."}
{"id": "PMID:1080415", "title": "[Linear measurements of the rhomboid fossa (the fourth ventricle in man].", "content": "The linear sizes of the rhombiod fossa of the human brain were studied with the help of the M. M. Sauliak-Savitskaja's device. Under study were 72 objects of the brain of humans from 20 to 78 years of age. The obtained data were statistically treated. No relationship between the sizes of the rhombiod fossa, sex and age was revealed, no statistically significant distinctions between the sizes of the right and left parts of the rhombiod fossa were revealed either. Between the length and width of the rhomboid fossa there exits a direct correlative relationship, the width of the rhomboid fossa having more close correlative relationships with the length of the superior part of the fossa rombiodea that the length of its inferior part.", "contents": "[Linear measurements of the rhomboid fossa (the fourth ventricle in man]. The linear sizes of the rhombiod fossa of the human brain were studied with the help of the M. M. Sauliak-Savitskaja's device. Under study were 72 objects of the brain of humans from 20 to 78 years of age. The obtained data were statistically treated. No relationship between the sizes of the rhombiod fossa, sex and age was revealed, no statistically significant distinctions between the sizes of the right and left parts of the rhombiod fossa were revealed either. Between the length and width of the rhomboid fossa there exits a direct correlative relationship, the width of the rhomboid fossa having more close correlative relationships with the length of the superior part of the fossa rombiodea that the length of its inferior part."}
{"id": "PMID:1080416", "title": "Pancreatic cyst complicated by major arterial erosion and gastrointestinal haemorrhage.", "content": "Uncommonly, pancreatic cysts are complicated by the erosion of certain adjacent arteries and serious gastrointestinal haemorrhage. This diagnosis should be entertained in any patient with chronic pancreatitis who presents with unexplained gastrointestinal blood loss, whether acute or chronic, a pulsatile mass in the epigastrium, and an associated bruit. Selective caeliac axis angiography may not only confirm the diagnosis, but provide precise anatomical information as a guide to the surgeon in planning treatment. The surgical treatment of choice is transcystic ligation of the bleeding vessel, followed by internal cyst drainage.", "contents": "Pancreatic cyst complicated by major arterial erosion and gastrointestinal haemorrhage. Uncommonly, pancreatic cysts are complicated by the erosion of certain adjacent arteries and serious gastrointestinal haemorrhage. This diagnosis should be entertained in any patient with chronic pancreatitis who presents with unexplained gastrointestinal blood loss, whether acute or chronic, a pulsatile mass in the epigastrium, and an associated bruit. Selective caeliac axis angiography may not only confirm the diagnosis, but provide precise anatomical information as a guide to the surgeon in planning treatment. The surgical treatment of choice is transcystic ligation of the bleeding vessel, followed by internal cyst drainage."}
{"id": "PMID:1080418", "title": "Attempted suicide: an operant formulation.", "content": "An alternative formulation of attempted suicide is offered, in which the phenomenon is considered within the context of social learning theory. The suicidal behaviour is viewed as an operant which is reinforced and maintained by the consequent interpersonal contingencies. The modification of the suicidal behaviour then involves alteration of the contingent consequences. This theoretical formulation is elaborated and the treatment principles and practice are outlined.", "contents": "Attempted suicide: an operant formulation. An alternative formulation of attempted suicide is offered, in which the phenomenon is considered within the context of social learning theory. The suicidal behaviour is viewed as an operant which is reinforced and maintained by the consequent interpersonal contingencies. The modification of the suicidal behaviour then involves alteration of the contingent consequences. This theoretical formulation is elaborated and the treatment principles and practice are outlined."}
{"id": "PMID:1080419", "title": "Immunoassay of serum polypeptide hormones by using 125I-labelled anti(-immunoglobulin G) antibodies.", "content": "1. A technique for indirectly labelling antibodies to polypeptide hormones, by combining them with radioactively labelled anti-(immunoglobulin G) is described. (a) 125I-labelled  anti-(rabbit immunoglobulin G) and anti-(guinea-pig immunoglobulin G) antibodies with high specific radioactivity were prepared after purification of the antibodies on immunoadsorbents containing the respective antigens. (b) Rabbit immunoglobulin G antibodies to human growth hormone, porcine glucagon and guinea-pig immunoglobulin G antibodies to bovine insulin and bovine parathyroid hormone were combined with immunoadsorbents containing the respective polypeptide hormone antigen. (c) The immunoglobulin G antibodies to the polypeptide hormones were reacted with 125-I-labelled anti-(immunoglobulin G) antibodies directed against the appropriate species of immunoglobulin G,and the anti-hormone antibodies were combined with the hormone-containing immunoadsorbent. (d) 125I-labelled anti-(immunoglobulin G) antibodies and anti-hormone antibodies were simultaneously eluted from the hormone-containing immunoadsorbent by dilute HCl, pH 2.0. After elution the anti-(immunoglobulin G) antibodies and antihormone antibodies were allowed to recombine at pH 8.0 and 4 degrees C. 2. The resultant immunoglobulin G-anti-immunoglobulin G complex was used in immunoradiometric (labelled antibody) and two-site assays of the respective polypeptide hormone. 3. By using these immunoassays, concentrations down to 90pg of human growth hormone/ml, 100 pg of bovine insulin/ml, 80 pg of bovine parathyroid hormone/ml and 150 pg of glucagon/ml were readily detected. Assays of human plasma for growth hormone and insulin by these methods showed good agreement with results obtained by using a directly 125I-labelled anti-hormone antibody in an immunoradiometric assay of human growth hormone or by radioimmunoassay of human insulin. 4. The method described allows immunoradiometric or two-site assays to be performed starting with as little as 450 ng of polypeptide hormone-antibody protein. An additional advantage of the method is that a single iodination of the readily available antibodies to immunoglobulin G allows the establishemnt of several polypeptide hormone assays", "contents": "Immunoassay of serum polypeptide hormones by using 125I-labelled anti(-immunoglobulin G) antibodies. 1. A technique for indirectly labelling antibodies to polypeptide hormones, by combining them with radioactively labelled anti-(immunoglobulin G) is described. (a) 125I-labelled  anti-(rabbit immunoglobulin G) and anti-(guinea-pig immunoglobulin G) antibodies with high specific radioactivity were prepared after purification of the antibodies on immunoadsorbents containing the respective antigens. (b) Rabbit immunoglobulin G antibodies to human growth hormone, porcine glucagon and guinea-pig immunoglobulin G antibodies to bovine insulin and bovine parathyroid hormone were combined with immunoadsorbents containing the respective polypeptide hormone antigen. (c) The immunoglobulin G antibodies to the polypeptide hormones were reacted with 125-I-labelled anti-(immunoglobulin G) antibodies directed against the appropriate species of immunoglobulin G,and the anti-hormone antibodies were combined with the hormone-containing immunoadsorbent. (d) 125I-labelled anti-(immunoglobulin G) antibodies and anti-hormone antibodies were simultaneously eluted from the hormone-containing immunoadsorbent by dilute HCl, pH 2.0. After elution the anti-(immunoglobulin G) antibodies and antihormone antibodies were allowed to recombine at pH 8.0 and 4 degrees C. 2. The resultant immunoglobulin G-anti-immunoglobulin G complex was used in immunoradiometric (labelled antibody) and two-site assays of the respective polypeptide hormone. 3. By using these immunoassays, concentrations down to 90pg of human growth hormone/ml, 100 pg of bovine insulin/ml, 80 pg of bovine parathyroid hormone/ml and 150 pg of glucagon/ml were readily detected. Assays of human plasma for growth hormone and insulin by these methods showed good agreement with results obtained by using a directly 125I-labelled anti-hormone antibody in an immunoradiometric assay of human growth hormone or by radioimmunoassay of human insulin. 4. The method described allows immunoradiometric or two-site assays to be performed starting with as little as 450 ng of polypeptide hormone-antibody protein. An additional advantage of the method is that a single iodination of the readily available antibodies to immunoglobulin G allows the establishemnt of several polypeptide hormone assays"}
{"id": "PMID:1080420", "title": "Iron removal from transferrin by a cell-free amphibian system.", "content": "1. Transferrin-bound iron was released by a cell-free solution prepared from Rana catesbeiana immature erythrocytes, showing that cellular integrity is not necessarily a requirement for this process as previously thought. 2. The biological molecule(s) involved in the removal of iron from transferrin is relatively small.", "contents": "Iron removal from transferrin by a cell-free amphibian system. 1. Transferrin-bound iron was released by a cell-free solution prepared from Rana catesbeiana immature erythrocytes, showing that cellular integrity is not necessarily a requirement for this process as previously thought. 2. The biological molecule(s) involved in the removal of iron from transferrin is relatively small."}
{"id": "PMID:1080422", "title": "Effect of cytostatic drugs on nucleic acid synthesis in mouse thymocytes and chick embryo cells.", "content": "Cell suspensions of either mouse thymocytes or chick embryo cells were incubated with labelled precursors after preincubation with different cytostatic drugs. The total time of in vitro incubation was four hours. The ratio between the amount of incorporated labelled precursors and the total amount of DNA was determined and used to measure the cytostatic effect. All drugs used caused a marked depression of this ratio. The cytostatic drugs had no effect on the phosphorylation of thymidine.", "contents": "Effect of cytostatic drugs on nucleic acid synthesis in mouse thymocytes and chick embryo cells. Cell suspensions of either mouse thymocytes or chick embryo cells were incubated with labelled precursors after preincubation with different cytostatic drugs. The total time of in vitro incubation was four hours. The ratio between the amount of incorporated labelled precursors and the total amount of DNA was determined and used to measure the cytostatic effect. All drugs used caused a marked depression of this ratio. The cytostatic drugs had no effect on the phosphorylation of thymidine."}
{"id": "PMID:1080423", "title": "The effect of sex hormones on the growth of HeLa tumour nodules in male and female mice.", "content": "The effect of exogenous sex hormones on the cell mediated response in male and female mice has been studied by measuring the subcutaneous growth of HeLa tumour nodules and the variation in the total lymphocyte count. It was found that oestrogen treated male and female mice experienced a profound lymphopenia which was vary rapid in onset. Concurrent with the lymphopenia there was prolongation of HeLa tumour nodule growth in female mice, but not in males. A lymphopenia occurred in androgen treated male mice with subsequent prolongation of HeLa tumour nodule growth, and a lymphocytosis in female mice, with reduction of HeLa tumour nodule growth.", "contents": "The effect of sex hormones on the growth of HeLa tumour nodules in male and female mice. The effect of exogenous sex hormones on the cell mediated response in male and female mice has been studied by measuring the subcutaneous growth of HeLa tumour nodules and the variation in the total lymphocyte count. It was found that oestrogen treated male and female mice experienced a profound lymphopenia which was vary rapid in onset. Concurrent with the lymphopenia there was prolongation of HeLa tumour nodule growth in female mice, but not in males. A lymphopenia occurred in androgen treated male mice with subsequent prolongation of HeLa tumour nodule growth, and a lymphocytosis in female mice, with reduction of HeLa tumour nodule growth."}
{"id": "PMID:1080424", "title": "T and B cell populations in blood and lymph node in lymphoproliferative disease.", "content": "Lymph node and peripheral blood lymphocytes were studied simultaneously for surface markers of T and B cells in 22 patients with lymphoproliferative diseases and 8 patients with non-neoplastic lymphadenopathy. This resulted in the classification of the malignancy from involved lymph nodes into 4 groups. Six patients had B cell lymphomata with normal or strong immunofluorescent staining for surface membrane immunoglobulin; 8 patients had B cell chronic lymphocytic leukaemia with pale staining for surface membrane immunoglobulin; 5 patients had T cell lymphomata and 3 patients were not definitely classifiable. In 6 out of 8 patients with B cell CLL, histopathology of lymph nodes showed infiltration with well differentiated lymphocytes and in all T cell lymphomata, the infiltrating cells were poorly differentiated. By the use of these markers, malignant lymphocytes were identified in the circulation in only 3 out of 6 patients with B cell lymphoma, in all patients with B cell CLL but in none of those with T cell lymphoma or unclassifiable lymphoma. Therefore a more conclusive characterization of the malignant lymphocyte in lymphoproliferative diseases must include an examination of involved lymph nodes.", "contents": "T and B cell populations in blood and lymph node in lymphoproliferative disease. Lymph node and peripheral blood lymphocytes were studied simultaneously for surface markers of T and B cells in 22 patients with lymphoproliferative diseases and 8 patients with non-neoplastic lymphadenopathy. This resulted in the classification of the malignancy from involved lymph nodes into 4 groups. Six patients had B cell lymphomata with normal or strong immunofluorescent staining for surface membrane immunoglobulin; 8 patients had B cell chronic lymphocytic leukaemia with pale staining for surface membrane immunoglobulin; 5 patients had T cell lymphomata and 3 patients were not definitely classifiable. In 6 out of 8 patients with B cell CLL, histopathology of lymph nodes showed infiltration with well differentiated lymphocytes and in all T cell lymphomata, the infiltrating cells were poorly differentiated. By the use of these markers, malignant lymphocytes were identified in the circulation in only 3 out of 6 patients with B cell lymphoma, in all patients with B cell CLL but in none of those with T cell lymphoma or unclassifiable lymphoma. Therefore a more conclusive characterization of the malignant lymphocyte in lymphoproliferative diseases must include an examination of involved lymph nodes."}
{"id": "PMID:1080425", "title": "Detection of two further beta-type cytochromes in Rhodopseudomonas spheroides.", "content": "The photosynthetically-incompetent mutant V-2 of Rhodopseudomonas spheroides which is incapable of synthesising bacteriochlorophyll was grown aerobically under conditions of both high and low aeration. Potentiometric titration a at 560 nm minus 570 nm revealed the presence of several different components tentatively identified as b-type cytochromes. Two such components of oxidation-reduction midpoint potentials of +390 mV +/- 10 mV and +255 mV +/- 7mV have not previously been detected in membranes of Rps. spheroides. These components have also been resolved by difference spectra at controlled oxidation-reduction potentials and fourth derivative spectra. Neither component appeared to react with CO. With increasing aeration of the culture medium the relative concentration of these two b-type cytochromes diminished, whilst that of the a-type oxidase increased.", "contents": "Detection of two further beta-type cytochromes in Rhodopseudomonas spheroides. The photosynthetically-incompetent mutant V-2 of Rhodopseudomonas spheroides which is incapable of synthesising bacteriochlorophyll was grown aerobically under conditions of both high and low aeration. Potentiometric titration a at 560 nm minus 570 nm revealed the presence of several different components tentatively identified as b-type cytochromes. Two such components of oxidation-reduction midpoint potentials of +390 mV +/- 10 mV and +255 mV +/- 7mV have not previously been detected in membranes of Rps. spheroides. These components have also been resolved by difference spectra at controlled oxidation-reduction potentials and fourth derivative spectra. Neither component appeared to react with CO. With increasing aeration of the culture medium the relative concentration of these two b-type cytochromes diminished, whilst that of the a-type oxidase increased."}
{"id": "PMID:1080421", "title": "Immunologic characterization of the mononuclear cell infiltrates in rheumatoid synovia, in rheumatoid nodules, and in lip biopsies from patients with Sj\u00f6gren's syndrome.", "content": "Two subcutaneous rheumatoid nodules and 8 rheumatoid synovia from patients with rheumatoid arthritis (RA); and 2 parotid glands, 1 \"pseudolymphoma,\" and 6 lip biopsies from patients with Sjogren's syndrome (SS) were studied to identify mononuclear cells. The palisading mononuclear cells in subcutaneous nodules had a surface membrane receptor for complement. B lymphocytes surrounded by larger numbers of non-B lymphocytes were found in RA synovium and between salivary ducts of SS lip biopsies. A \"pseudolymphoma\" obtained from a patient with SS consisted primarily of B lymphocytes. The predominant mononuclear cell in rheumatoid synovia and salivary glands in patients with RA and SS do not have surface membrane receptors from complement and are thus probably T lymphocytes or null cells.", "contents": "Immunologic characterization of the mononuclear cell infiltrates in rheumatoid synovia, in rheumatoid nodules, and in lip biopsies from patients with Sj\u00f6gren's syndrome. Two subcutaneous rheumatoid nodules and 8 rheumatoid synovia from patients with rheumatoid arthritis (RA); and 2 parotid glands, 1 \"pseudolymphoma,\" and 6 lip biopsies from patients with Sjogren's syndrome (SS) were studied to identify mononuclear cells. The palisading mononuclear cells in subcutaneous nodules had a surface membrane receptor for complement. B lymphocytes surrounded by larger numbers of non-B lymphocytes were found in RA synovium and between salivary ducts of SS lip biopsies. A \"pseudolymphoma\" obtained from a patient with SS consisted primarily of B lymphocytes. The predominant mononuclear cell in rheumatoid synovia and salivary glands in patients with RA and SS do not have surface membrane receptors from complement and are thus probably T lymphocytes or null cells."}
{"id": "PMID:1080430", "title": "Experimental murine amyloidosis: a model system for studying amyloid formation.", "content": "Myeloma-associated and casein-induced murine amyloidosis were used as models to study the role of lymphocytes and macrophages in amyloid formation. Amyloidosis occurred rarely and in small amounts in Balb/C mice with immunoglobulin (Ig)-producing myeloma tumours but large amounts could be induced by injections of casein. Fluorescent staining of both forms of amyloid deposits by means of anti-casein- and anti-myeloma-amyloid antibodies indicated that they either crossreacted or coexisted. Nor abnormality of Ig biosynthesis was detected in amyloidosis, suggesting that abnormal degradation was responsible for production of the Ig form of amyloid. Although spleen lymphocytes of casein-injected mice with amyloidosis demonstrated diminished cellular immunologic responses, this did not indicate generalized immunologic incompetence. The non-Ig form of amyloid in casein-injected mice was shown to be produced by macrophages, and a technique was developed for increasing the yield of amyloid-containing cells.", "contents": "Experimental murine amyloidosis: a model system for studying amyloid formation. Myeloma-associated and casein-induced murine amyloidosis were used as models to study the role of lymphocytes and macrophages in amyloid formation. Amyloidosis occurred rarely and in small amounts in Balb/C mice with immunoglobulin (Ig)-producing myeloma tumours but large amounts could be induced by injections of casein. Fluorescent staining of both forms of amyloid deposits by means of anti-casein- and anti-myeloma-amyloid antibodies indicated that they either crossreacted or coexisted. Nor abnormality of Ig biosynthesis was detected in amyloidosis, suggesting that abnormal degradation was responsible for production of the Ig form of amyloid. Although spleen lymphocytes of casein-injected mice with amyloidosis demonstrated diminished cellular immunologic responses, this did not indicate generalized immunologic incompetence. The non-Ig form of amyloid in casein-injected mice was shown to be produced by macrophages, and a technique was developed for increasing the yield of amyloid-containing cells."}
{"id": "PMID:1080431", "title": "Acute lymphocytic leukemia in children.", "content": "An overview is presented of the improvements in the prognosis of acute lymphocytic leukemia due to combined modality therapy. With the best available regimens, approximately 50% of these children have remained leukemia-free for 5 years or more. Because of these results, there is growing concern for the quality of survival and for the side effects of therapy. A case in point is a completely unexpected side effect in a current study. Nonleukemic leukoencephalopathy has developed in 8 of 20 children given intravenous methotrexate, 50-80 mg/m2 per week, as the sole agent following remission induction and CNS therapy. Thus, with longer remissions and survivals now commonly observed, a concerted effort is needed to minimize side effects while trying to improve further the efficacy of therapy.", "contents": "Acute lymphocytic leukemia in children. An overview is presented of the improvements in the prognosis of acute lymphocytic leukemia due to combined modality therapy. With the best available regimens, approximately 50% of these children have remained leukemia-free for 5 years or more. Because of these results, there is growing concern for the quality of survival and for the side effects of therapy. A case in point is a completely unexpected side effect in a current study. Nonleukemic leukoencephalopathy has developed in 8 of 20 children given intravenous methotrexate, 50-80 mg/m2 per week, as the sole agent following remission induction and CNS therapy. Thus, with longer remissions and survivals now commonly observed, a concerted effort is needed to minimize side effects while trying to improve further the efficacy of therapy."}
{"id": "PMID:1080432", "title": "Chemical synthesis of a peptide fragment of thymopoietin II that induces selective T cell differentiation.", "content": "Thymopoietin is a polypeptide hormone of the thymus that consists of a 49 amino acid polypeptide chain of 5562 daltons. A peptide corresponding to positions 29-41 of bovine thymopoietin II was synthesized by the Merrifield solid-phase technique. This peptide was shown to have a purity (correct sequence) of 96% by amino acid and C terminal analyses and by a complete determination of the amino acid sequence by manual Edman degradations. It displayed a selectivity of action similar to that of thymopoietin itself, inducing the differentiation of T lymphocytes but not of complement receptor (CR+) B lymphocytes. Although a number of substances induce the differentiation of both T cells and CR+B cells under the conditions of assay in vitro, only thymopoietin and the synthetic peptide described in this report have been shown to induce the differentiation of T cells selectively. Our data establish that the key residues involved in the active site of thymopoietin are present within a synthetic polypeptide which constitutes a minor portion of the amino acid sequence of thymopoietin. Since this peptide had 3% activity by comparison with thymopoietin, the tertiary structure of thymopoietin may be required for optimal configuration of the active site to produce full biological activity.", "contents": "Chemical synthesis of a peptide fragment of thymopoietin II that induces selective T cell differentiation. Thymopoietin is a polypeptide hormone of the thymus that consists of a 49 amino acid polypeptide chain of 5562 daltons. A peptide corresponding to positions 29-41 of bovine thymopoietin II was synthesized by the Merrifield solid-phase technique. This peptide was shown to have a purity (correct sequence) of 96% by amino acid and C terminal analyses and by a complete determination of the amino acid sequence by manual Edman degradations. It displayed a selectivity of action similar to that of thymopoietin itself, inducing the differentiation of T lymphocytes but not of complement receptor (CR+) B lymphocytes. Although a number of substances induce the differentiation of both T cells and CR+B cells under the conditions of assay in vitro, only thymopoietin and the synthetic peptide described in this report have been shown to induce the differentiation of T cells selectively. Our data establish that the key residues involved in the active site of thymopoietin are present within a synthetic polypeptide which constitutes a minor portion of the amino acid sequence of thymopoietin. Since this peptide had 3% activity by comparison with thymopoietin, the tertiary structure of thymopoietin may be required for optimal configuration of the active site to produce full biological activity."}
{"id": "PMID:1080436", "title": "Left main coronary artery obstruction. Follow-up of thirty patients with and without surgery.", "content": "Thirty patients, 20 males and 10 females, having a 50% or greater obstruction of the left main coronary artery, were evaluated. Their ages ranged from 40 to 71 years (mean 53.8 years). All patients had angina pectoris, the duration of which varied from 2 months to 20 years (mean 5.4 years). Twenty-four patients (80%) had accelerated angina pectoris; 17 (56.7%) had prior myocardial infarction, and 27 (90%) were found to have coexisting three-vessel disease and abnormal left ventricular contraction. There were no deaths or serious arrhythmias related to cardiac catheterization procedures. In the 11 unoperated patients 5 (45%) died within the first year of follow-up; however, all except one death occurred in patients with diffuse distal coronary artery disease technically unsuitable for surgery. Among the six survivors four were considered surgical candidates. These four patients have continued to have symptoms of angina pectoris with a mean follow-up period of 17.5 months (16 to 20 months). In the 19 patients who underwent aortocoronary saphenous vein bypass grafting, there was one immediate postoperative death (surgical mortality 5.3%) and one late death. The 17 survivors in the surgical group have a mean follow-up period of 17.3 months (8 to 31 months); 13 of them have clinically improved with 6 being totally free of angina pectoris. Thus, mortality is extremely high in those with poor distal vasculature technically unsuitable for surgery, but mortality is relatively low in patients who have technically bypassable lesions whether treated surgically or medically. Although saphenous vein bypass grafting appears to be more effective in providing clinical improvement, asymptomatic left main coronary obstruction may not justify surgical therapy.", "contents": "Left main coronary artery obstruction. Follow-up of thirty patients with and without surgery. Thirty patients, 20 males and 10 females, having a 50% or greater obstruction of the left main coronary artery, were evaluated. Their ages ranged from 40 to 71 years (mean 53.8 years). All patients had angina pectoris, the duration of which varied from 2 months to 20 years (mean 5.4 years). Twenty-four patients (80%) had accelerated angina pectoris; 17 (56.7%) had prior myocardial infarction, and 27 (90%) were found to have coexisting three-vessel disease and abnormal left ventricular contraction. There were no deaths or serious arrhythmias related to cardiac catheterization procedures. In the 11 unoperated patients 5 (45%) died within the first year of follow-up; however, all except one death occurred in patients with diffuse distal coronary artery disease technically unsuitable for surgery. Among the six survivors four were considered surgical candidates. These four patients have continued to have symptoms of angina pectoris with a mean follow-up period of 17.5 months (16 to 20 months). In the 19 patients who underwent aortocoronary saphenous vein bypass grafting, there was one immediate postoperative death (surgical mortality 5.3%) and one late death. The 17 survivors in the surgical group have a mean follow-up period of 17.3 months (8 to 31 months); 13 of them have clinically improved with 6 being totally free of angina pectoris. Thus, mortality is extremely high in those with poor distal vasculature technically unsuitable for surgery, but mortality is relatively low in patients who have technically bypassable lesions whether treated surgically or medically. Although saphenous vein bypass grafting appears to be more effective in providing clinical improvement, asymptomatic left main coronary obstruction may not justify surgical therapy."}
{"id": "PMID:1080437", "title": "Myocardial damage in combined valvular and coronary bypass surgery.", "content": "The incidence of perioperative myocardial infarction (MI) and acute ischemic injury was determined in 44 patients having aortic valve replacement plus aortocoronary vein grafts (AVR + CABG) and in 22 patients having mitral valve repair or replacement plus aortocoronary vein grafts (MVR + CABG). These data were correlated with aortic cross-clamp time, fibrillation time, presence of left ventricular hypertrophy, and, in selected cases, left ventricular biopsy. Perioperative MI occurred in 21% (9/44) of the AVR + CABG patients and 5% (1/22) of MVR + CABG patients. In contrast, the MI rate for isolated valve replacement was 7% (15/213), and for isolated CABG 14% (16/112). In the AVR + CABG group, 100% (5/5) of patients with combined cross-clamp and fibrillation times greater than 120 minutes suffered MI. These results indicate that patients with combined AVR + CABG are subject to substantially greater risk of perioperative myocardial damage than the other groups. Excessive aortic cross-clamp times and fibrillation plus aortic cross-clamp times should be avoided in AVR + CABG patients.", "contents": "Myocardial damage in combined valvular and coronary bypass surgery. The incidence of perioperative myocardial infarction (MI) and acute ischemic injury was determined in 44 patients having aortic valve replacement plus aortocoronary vein grafts (AVR + CABG) and in 22 patients having mitral valve repair or replacement plus aortocoronary vein grafts (MVR + CABG). These data were correlated with aortic cross-clamp time, fibrillation time, presence of left ventricular hypertrophy, and, in selected cases, left ventricular biopsy. Perioperative MI occurred in 21% (9/44) of the AVR + CABG patients and 5% (1/22) of MVR + CABG patients. In contrast, the MI rate for isolated valve replacement was 7% (15/213), and for isolated CABG 14% (16/112). In the AVR + CABG group, 100% (5/5) of patients with combined cross-clamp and fibrillation times greater than 120 minutes suffered MI. These results indicate that patients with combined AVR + CABG are subject to substantially greater risk of perioperative myocardial damage than the other groups. Excessive aortic cross-clamp times and fibrillation plus aortic cross-clamp times should be avoided in AVR + CABG patients."}
{"id": "PMID:1080438", "title": "Prospective randomized study of coronary bypass surgery in stable angina. The first 100 patients.", "content": "In a prospective study to evaluate surgery for angina pectoris, 50 patients randomized to surgery and 50 treated medically have completed 8 to 34 months' follow-up (median, 24 months). Anginal symptoms improved in both groups (88%, 72%), but more operated patients are currently asymptomatic (70%, 8%) and using fewer medications (nitrates, 18%, 96%; propranolol, 8%, 72% of patients). Exercise tests with treadmill revealed increased exercise tolerance time in both groups, more so in the operated group (+ 89%, + 42%), and angina could not be provoked in 70% and 20% of patients, respectively. During the follow-up, fewer cardiac complications developed in the operated group (19 vs. 44; P = less than 0.02); there was no difference in mortality. Repeat catheterization in 83 of 84 survivors at 1 year did not reveal any significant differences in the progression of disease. Atrial pacing studies revealed improvement in anginal threshold in 70% of operated and 48% of nonoperated patients. The study suggests that although subjective and objective improvement occurs with surgical as well as nonsurgical treatment, the frequency and magnitude are higher with surgery. The quality of life appears to be better in the operated patients during the first 24 months. Further follow-up is necessary to determine the duration of such improvement and to assess the influence of surgery on the natural life history of this disease.", "contents": "Prospective randomized study of coronary bypass surgery in stable angina. The first 100 patients. In a prospective study to evaluate surgery for angina pectoris, 50 patients randomized to surgery and 50 treated medically have completed 8 to 34 months' follow-up (median, 24 months). Anginal symptoms improved in both groups (88%, 72%), but more operated patients are currently asymptomatic (70%, 8%) and using fewer medications (nitrates, 18%, 96%; propranolol, 8%, 72% of patients). Exercise tests with treadmill revealed increased exercise tolerance time in both groups, more so in the operated group (+ 89%, + 42%), and angina could not be provoked in 70% and 20% of patients, respectively. During the follow-up, fewer cardiac complications developed in the operated group (19 vs. 44; P = less than 0.02); there was no difference in mortality. Repeat catheterization in 83 of 84 survivors at 1 year did not reveal any significant differences in the progression of disease. Atrial pacing studies revealed improvement in anginal threshold in 70% of operated and 48% of nonoperated patients. The study suggests that although subjective and objective improvement occurs with surgical as well as nonsurgical treatment, the frequency and magnitude are higher with surgery. The quality of life appears to be better in the operated patients during the first 24 months. Further follow-up is necessary to determine the duration of such improvement and to assess the influence of surgery on the natural life history of this disease."}
{"id": "PMID:1080439", "title": "Long-term survival following coronary bypass surgery in patients with significant impairment of left ventricular function.", "content": "To assess the influence of coronary revascularization on the long-term survival of patients with debilitating angina and significant impairment of left ventricular function, analysis was done of 62 consecutive patients with severe angina and hypokinetic left ventricles (LVED larger than or equal to 20) undergoing coronary bypass at N.Y.U. Medical Center between January, 1971 and May 1974. Follow-up was 98% complete, range 4 to 41 months with a mean of 23 months. Multiple bypasses were done in 94% of the group; a left-ventricular scar was excised in 16%. There were six operative deaths (mortality 9.7%) and three late deaths, all within eight months after operation. Angina was either absent or substantially improved in 90% of surviving patients. Life-table analysis shows a two-year survival of 85%, very similar to the survival rate for patients with good ventricular function. Hence, with current techniques impaired ventricular function is not a major contraindication to bypass grafting.", "contents": "Long-term survival following coronary bypass surgery in patients with significant impairment of left ventricular function. To assess the influence of coronary revascularization on the long-term survival of patients with debilitating angina and significant impairment of left ventricular function, analysis was done of 62 consecutive patients with severe angina and hypokinetic left ventricles (LVED larger than or equal to 20) undergoing coronary bypass at N.Y.U. Medical Center between January, 1971 and May 1974. Follow-up was 98% complete, range 4 to 41 months with a mean of 23 months. Multiple bypasses were done in 94% of the group; a left-ventricular scar was excised in 16%. There were six operative deaths (mortality 9.7%) and three late deaths, all within eight months after operation. Angina was either absent or substantially improved in 90% of surviving patients. Life-table analysis shows a two-year survival of 85%, very similar to the survival rate for patients with good ventricular function. Hence, with current techniques impaired ventricular function is not a major contraindication to bypass grafting."}
{"id": "PMID:1080440", "title": "Effects of partial obstruction on phasic flow in aortocoronary grafts.", "content": "Phasic blood flow recorded in five unobstructed dog aortocoronary bypass grafts to the left coronary artery was similar to that of normal coronary arteries, with a high diastolic-to-systolic flow ratio which averaged 2.7 +/- 0.4. With an average of 52 +/- 7% and 72 +/- 6% stenosis in the coronary artery distal to the graft, the phasic graft-flow pattern was altered, with a decrease in the diastolic-to-systolic flow ratio to 1.9 +/- 0.4 and 1.2 +/- 0.4, respectively. Phasic patterns recorded in 42 human saphenous vein grafts to branches of the left coronary artery subsequently shown to be patent by postoperative coronary arteriography were similar to those of a normal coronary artery, with an average diastolic-to-systolic flow ratio of 2.3 +/- 0.83 and an average flow of 76 +/- 48 ml/min. Phasic flow measured in 20 grafts shown to be occulded at restudy had an average diastolic-to-systolic flow of 1.3 +/- 0.45, with an average flow of 72 +/- 33 ml/min and a phasic graft flow which was similar to that obtained from partially obstructed coronary arteries. We conclude that partial obstruction of the graft or the distal coronary artery alters the flow pattern, and this partial obstruction may lead to closure of the graft.", "contents": "Effects of partial obstruction on phasic flow in aortocoronary grafts. Phasic blood flow recorded in five unobstructed dog aortocoronary bypass grafts to the left coronary artery was similar to that of normal coronary arteries, with a high diastolic-to-systolic flow ratio which averaged 2.7 +/- 0.4. With an average of 52 +/- 7% and 72 +/- 6% stenosis in the coronary artery distal to the graft, the phasic graft-flow pattern was altered, with a decrease in the diastolic-to-systolic flow ratio to 1.9 +/- 0.4 and 1.2 +/- 0.4, respectively. Phasic patterns recorded in 42 human saphenous vein grafts to branches of the left coronary artery subsequently shown to be patent by postoperative coronary arteriography were similar to those of a normal coronary artery, with an average diastolic-to-systolic flow ratio of 2.3 +/- 0.83 and an average flow of 76 +/- 48 ml/min. Phasic flow measured in 20 grafts shown to be occulded at restudy had an average diastolic-to-systolic flow of 1.3 +/- 0.45, with an average flow of 72 +/- 33 ml/min and a phasic graft flow which was similar to that obtained from partially obstructed coronary arteries. We conclude that partial obstruction of the graft or the distal coronary artery alters the flow pattern, and this partial obstruction may lead to closure of the graft."}
{"id": "PMID:1080441", "title": "Coronary bypass surgery. Value of maximal exercise testing in assessment of results.", "content": "To evaluate the relationship of results of maximal treadmill testing and results of saphenous vein bypass surgery (SVG), 30 patients underwent a maximal treadmill test before and after SVG; an additional 39 had results of postoperative exercise testing available. These patients were part of a group of 91 patients (95% of 96 survivors) who had complete right- and left-heart catheterizations and coronary angiography before and after operation. Twenty-one per cent had improved ejection fractions postoperatively; in 32% ejection fractions were worse. Duration of maximal treadmill exercise correlated poorly with ejection fraction changes or graft patency. Rate-pressure product (RPP) was usually lower postoperatively in patients with worse ejection fractions, primarily because of a low systolic blood pressure at maximum exercise. RPP postoperatively of less than 25,000 almost always meant poor surgical results, either graft occlusion or poor left ventricular function, while RPP of more than 30,000 meant that left ventricular function was preserved and all grafts or the graft to the anterior descending were patent. Thus, success or failure of SVG in these groups may be predicted without postoperative catheterization. An open graft to the anterior descending coronary generally insured a high RPP with exercise, even if other grafts were closed, as long as ventricular function remained good.", "contents": "Coronary bypass surgery. Value of maximal exercise testing in assessment of results. To evaluate the relationship of results of maximal treadmill testing and results of saphenous vein bypass surgery (SVG), 30 patients underwent a maximal treadmill test before and after SVG; an additional 39 had results of postoperative exercise testing available. These patients were part of a group of 91 patients (95% of 96 survivors) who had complete right- and left-heart catheterizations and coronary angiography before and after operation. Twenty-one per cent had improved ejection fractions postoperatively; in 32% ejection fractions were worse. Duration of maximal treadmill exercise correlated poorly with ejection fraction changes or graft patency. Rate-pressure product (RPP) was usually lower postoperatively in patients with worse ejection fractions, primarily because of a low systolic blood pressure at maximum exercise. RPP postoperatively of less than 25,000 almost always meant poor surgical results, either graft occlusion or poor left ventricular function, while RPP of more than 30,000 meant that left ventricular function was preserved and all grafts or the graft to the anterior descending were patent. Thus, success or failure of SVG in these groups may be predicted without postoperative catheterization. An open graft to the anterior descending coronary generally insured a high RPP with exercise, even if other grafts were closed, as long as ventricular function remained good."}
{"id": "PMID:1080442", "title": "Combined gas endarterectomy and coronary artery bypass graft. A follow-up study.", "content": "One hundred and four patients who underwent combined gas endarterectomy and bypass grafting were followed for periods up to 4 1/2 years (mean, 15 months). The early mortality was 2.9% and the late mortality 3.8%; all of these deaths occurred in patients operated on for heart failure. The survival rate, presented in an actuarial manner, was 91% for the whole group at 3 years. Perioperative infarction occurred in 5.8%, was usually accompanied by patent grafts, and produced left ventricular dysfunction in only 1 of 5 patients investigated. Symptomatic improvement was observed in 96% of the patients. There was fairly good correlation between symptomatic improvement and graft patency. Repeat graft and coronary angiography showed that of 65 grafts to endartectomized arteries, 56 were patent (a patency rate of 87%); there was no evidence of decrease of patency rate with time, and no significant difference between patency rates of grafts to different arteries. The runoff of patent grafts was judged to be good in 75% of patients, moderate in 15% and poor in 10%.", "contents": "Combined gas endarterectomy and coronary artery bypass graft. A follow-up study. One hundred and four patients who underwent combined gas endarterectomy and bypass grafting were followed for periods up to 4 1/2 years (mean, 15 months). The early mortality was 2.9% and the late mortality 3.8%; all of these deaths occurred in patients operated on for heart failure. The survival rate, presented in an actuarial manner, was 91% for the whole group at 3 years. Perioperative infarction occurred in 5.8%, was usually accompanied by patent grafts, and produced left ventricular dysfunction in only 1 of 5 patients investigated. Symptomatic improvement was observed in 96% of the patients. There was fairly good correlation between symptomatic improvement and graft patency. Repeat graft and coronary angiography showed that of 65 grafts to endartectomized arteries, 56 were patent (a patency rate of 87%); there was no evidence of decrease of patency rate with time, and no significant difference between patency rates of grafts to different arteries. The runoff of patent grafts was judged to be good in 75% of patients, moderate in 15% and poor in 10%."}
{"id": "PMID:1080443", "title": "Myocardial infarction in coronary artery surgery.", "content": "Myocardial infarction has been noted as a frequent complication of coronary artery surgery in many review series, although its causes are uncertain. Follow-up of 100 patients at 19.7 months (mean) after coronary bypass surgery identified 15 patients with perioperative myocardial infarction as judged by new, significant Q waves after surgery. There were no significnat differences in age, preoperative anginal class, previous infarction, presence of hypertension, hyperlipidemia, or frank glucose intolerance compared with the 85 patients without infarction. Nor was there a significnat difference in coronary artery score, left ventricular end-diastolic pressure, cardiac index, or presence of collaterals. Cardiopulmonary bypass time, duration of anoxic arrest, and number of vessels grafted did not differ. Perioperative infarction always occurred in the territory of a grafted vessel and not in comparably compromised, nongrafted areas. In 13 cases new Q waves appeared in the first 24 hours, and myocardial infarction was not clinically suspected. Eight of the 15 grafts at risk were patent at late follow-up. Mean ejection fraction was not significantly changed postoperatively, but affected segmental wall motion declined in most cases. Five patients with perioperative infarction but no patent grafts improved by only 1 NYHA class (mean), but ten patients with infarction and one or more grafts patent improved by 2.9 NYHA classes (mean). Perioperative infarction could not be correlated with currently recognized patient and operative risk factors. The consistent anatomical relationship suggested that the grafting procedure itself was critical to the occurrence of distal segmental infarction.", "contents": "Myocardial infarction in coronary artery surgery. Myocardial infarction has been noted as a frequent complication of coronary artery surgery in many review series, although its causes are uncertain. Follow-up of 100 patients at 19.7 months (mean) after coronary bypass surgery identified 15 patients with perioperative myocardial infarction as judged by new, significant Q waves after surgery. There were no significnat differences in age, preoperative anginal class, previous infarction, presence of hypertension, hyperlipidemia, or frank glucose intolerance compared with the 85 patients without infarction. Nor was there a significnat difference in coronary artery score, left ventricular end-diastolic pressure, cardiac index, or presence of collaterals. Cardiopulmonary bypass time, duration of anoxic arrest, and number of vessels grafted did not differ. Perioperative infarction always occurred in the territory of a grafted vessel and not in comparably compromised, nongrafted areas. In 13 cases new Q waves appeared in the first 24 hours, and myocardial infarction was not clinically suspected. Eight of the 15 grafts at risk were patent at late follow-up. Mean ejection fraction was not significantly changed postoperatively, but affected segmental wall motion declined in most cases. Five patients with perioperative infarction but no patent grafts improved by only 1 NYHA class (mean), but ten patients with infarction and one or more grafts patent improved by 2.9 NYHA classes (mean). Perioperative infarction could not be correlated with currently recognized patient and operative risk factors. The consistent anatomical relationship suggested that the grafting procedure itself was critical to the occurrence of distal segmental infarction."}
{"id": "PMID:1080444", "title": "Myocardial blood flow following saphenous vein bypass surgery.", "content": "Myocardial blood flow was measured by the 133Xe clearance technique in 21 patients 6 to 31 months after saphenous vein grafting for coronary arterial disease. Preoperative measurements were made in 16. All patients had improved symptomatically, and 20 had returned to work. Thirty of 39 grafts were entered and selective injections of 133Xe performed. Flow in the left coronary artery (LCA) was unchanged postoperatively in 9, increased in 5, and decreased in 1 (preoperative mean = 54 ml/100 g/min, SD +/- 16; postoperative mean = 65 ml/100 g/min, SD +/- 20). Flow in the right coronary artery (RCA) was unchanged postoperatively in 3, decreased in 9, and increased in 2 (preoperative mean = 46 ml/100 g/min, SD +/- 18; postoperative mean = 33 ml/100 g/min, SD +/- 12). Nine of 14 grafts to the left anterior descending coronary artery showed flow rates significantly greater than those in the corresponding LCA. Two of the 3 circumflex grafts showed flow rates significantly lower than those in the LCA. Four of 8 right grafts showed significantly higher flow rates than those in the corresponding RCA. These findings correlated well with the angiographic appearances. We conclude that saphenous vein grafting increases total myocardial blood flow. The data suggest that the coronary arteries and the grafts are supplying different regions of the myocardium, and that the graft regions were previously underperfused. There would appear to be little mixing of blood between artery and graft regions.", "contents": "Myocardial blood flow following saphenous vein bypass surgery. Myocardial blood flow was measured by the 133Xe clearance technique in 21 patients 6 to 31 months after saphenous vein grafting for coronary arterial disease. Preoperative measurements were made in 16. All patients had improved symptomatically, and 20 had returned to work. Thirty of 39 grafts were entered and selective injections of 133Xe performed. Flow in the left coronary artery (LCA) was unchanged postoperatively in 9, increased in 5, and decreased in 1 (preoperative mean = 54 ml/100 g/min, SD +/- 16; postoperative mean = 65 ml/100 g/min, SD +/- 20). Flow in the right coronary artery (RCA) was unchanged postoperatively in 3, decreased in 9, and increased in 2 (preoperative mean = 46 ml/100 g/min, SD +/- 18; postoperative mean = 33 ml/100 g/min, SD +/- 12). Nine of 14 grafts to the left anterior descending coronary artery showed flow rates significantly greater than those in the corresponding LCA. Two of the 3 circumflex grafts showed flow rates significantly lower than those in the LCA. Four of 8 right grafts showed significantly higher flow rates than those in the corresponding RCA. These findings correlated well with the angiographic appearances. We conclude that saphenous vein grafting increases total myocardial blood flow. The data suggest that the coronary arteries and the grafts are supplying different regions of the myocardium, and that the graft regions were previously underperfused. There would appear to be little mixing of blood between artery and graft regions."}
{"id": "PMID:1080445", "title": "Saphenous vein bypass grafting. Changes in native circulation and collaterals.", "content": "One hundred patients were evaluated 12 to 43 months after saphenous vein bypass surgery. The rate of progression of obstructive disease in the native vessels and changes in collateral circulation were analyzed in detail. Fifty-five per cent of preoperatively patent vessels showed progression proximal to the site of graft insertion, 40% proximal to patent grafts; there was distal progression in only 7% of grafted vessels. The progression rate for nongrafted vessels was 14%. Patent grafts were associated with a high rate of disappearance of collaterals to the grafted vessels, while occluded grafts were associated with preservation of collaterals or appearance of new collaterals. Progression of obstructive disease in the native circulation and changes in collaterals did not prevent symptomatic improvement (93% of patients were symptomatically improved).", "contents": "Saphenous vein bypass grafting. Changes in native circulation and collaterals. One hundred patients were evaluated 12 to 43 months after saphenous vein bypass surgery. The rate of progression of obstructive disease in the native vessels and changes in collateral circulation were analyzed in detail. Fifty-five per cent of preoperatively patent vessels showed progression proximal to the site of graft insertion, 40% proximal to patent grafts; there was distal progression in only 7% of grafted vessels. The progression rate for nongrafted vessels was 14%. Patent grafts were associated with a high rate of disappearance of collaterals to the grafted vessels, while occluded grafts were associated with preservation of collaterals or appearance of new collaterals. Progression of obstructive disease in the native circulation and changes in collaterals did not prevent symptomatic improvement (93% of patients were symptomatically improved)."}
{"id": "PMID:1080446", "title": "Improved angina threshold and coronary reserve following direct myocardial revascularization.", "content": "Angina threshold, coronary reserve, and global myocardial lactate metabolism were studied by atrial pacing in 18 patients with obstructive coronary artery disease before and after aortocoronary artery bypass (ACB) surgery. In 3 of these 18 patients, regional (anterior wall) metabolism was also studied. Following ACB, 16 of the 18 patients did not develop angina at the maximum pacing rate (MPR). One patient developed angina postoperatively at a similar rate as before surgery. In the other patient, postoperative angina threshold was much higher. In the group as a whole, postoperative MPR (159 +/- 3.5 beats/min) was much higher than the preoperative angina rate (124.9 +/- 4.9 beats/min; P less than 0.001). Rate-pressure product (RP) at MPR postoperatively (21.5 +/- 0.89 mm Hg/min X 10(-3)) was also higher than RP at angina rate preoperatively (18.8 +/- 0.92 mm Hg/min X 10(-3); P less than 0.01). Although coronary sinus blood flow (CSBF) both at rest (152 +/- 16.2 ml/min) and at MRP (266 +/- 27.5 ml/min) postoperatively was higher than preoperative CSBF at rest (111 +/- 10.7 ml/min; P less than 0.05) and at angina rate (202 +/- 19.9 ml/min; P less than 0.05), arterial-coronary sinus O2 content (Art.-CSO2) difference was significantly lower postoperatively both at rest (8.9 +/- 0.37 ml/min) and at MPR (9.1 +/- 0.44 ml/min) compared with the preoperative Art.-CSO2 at the rest (12.7 +/- 0.40 ml/min; P less than 0.01) and at angina rate (12.4 +/- 3.8 ml/min; P less than 0.01)...", "contents": "Improved angina threshold and coronary reserve following direct myocardial revascularization. Angina threshold, coronary reserve, and global myocardial lactate metabolism were studied by atrial pacing in 18 patients with obstructive coronary artery disease before and after aortocoronary artery bypass (ACB) surgery. In 3 of these 18 patients, regional (anterior wall) metabolism was also studied. Following ACB, 16 of the 18 patients did not develop angina at the maximum pacing rate (MPR). One patient developed angina postoperatively at a similar rate as before surgery. In the other patient, postoperative angina threshold was much higher. In the group as a whole, postoperative MPR (159 +/- 3.5 beats/min) was much higher than the preoperative angina rate (124.9 +/- 4.9 beats/min; P less than 0.001). Rate-pressure product (RP) at MPR postoperatively (21.5 +/- 0.89 mm Hg/min X 10(-3)) was also higher than RP at angina rate preoperatively (18.8 +/- 0.92 mm Hg/min X 10(-3); P less than 0.01). Although coronary sinus blood flow (CSBF) both at rest (152 +/- 16.2 ml/min) and at MRP (266 +/- 27.5 ml/min) postoperatively was higher than preoperative CSBF at rest (111 +/- 10.7 ml/min; P less than 0.05) and at angina rate (202 +/- 19.9 ml/min; P less than 0.05), arterial-coronary sinus O2 content (Art.-CSO2) difference was significantly lower postoperatively both at rest (8.9 +/- 0.37 ml/min) and at MPR (9.1 +/- 0.44 ml/min) compared with the preoperative Art.-CSO2 at the rest (12.7 +/- 0.40 ml/min; P less than 0.01) and at angina rate (12.4 +/- 3.8 ml/min; P less than 0.01)..."}
{"id": "PMID:1080447", "title": "Changes in survival and symptom relief in a longitudinal study of patients after bypass surgery.", "content": "The first 350 patients having coronary surgery at Stanford Medical Center (excluding patients with aneurysmectomy or valve replacement) have been followed sequentially utilizing a computer-based information system. Our experience spans 72 months (900 patient-years), with an average follow-up of 30 months. Hospital mortality was 4.9%. Actuarial analysis revealed survival of 91% at 1 year and 80% at 6 years. Forty patients (11.5%) had 43 late myocardial infarctions, of which 5 were fatal. Analyses of selected patient subgroups revealed significantly poorer survival in patients with prior myocardial infarction (P less than 0.05), significant congestive heart failure, or mitral regurgitation (P less than 0.01). Survival in multigrafted (and multivessel) patients was not significantly different from survival in single-grafted (predominantly single-vessel) patients. Actuarial studies suggest improved survival in patients with multivessel disease after coronary artery surgery. Between an initial evaluation at 9 months postoperatively (range: 2 to 40 months) and the most recent evaluation after 30 months (range: 6 to 72 months), 13% of patients showed further clinical improvement, 47% were unchanged, while 40% deteriorated with respect to chest pain. We conclude that initial symptomatic benefits may not be maintained in late follow-up studies owing to progression of underlying vascular disease.", "contents": "Changes in survival and symptom relief in a longitudinal study of patients after bypass surgery. The first 350 patients having coronary surgery at Stanford Medical Center (excluding patients with aneurysmectomy or valve replacement) have been followed sequentially utilizing a computer-based information system. Our experience spans 72 months (900 patient-years), with an average follow-up of 30 months. Hospital mortality was 4.9%. Actuarial analysis revealed survival of 91% at 1 year and 80% at 6 years. Forty patients (11.5%) had 43 late myocardial infarctions, of which 5 were fatal. Analyses of selected patient subgroups revealed significantly poorer survival in patients with prior myocardial infarction (P less than 0.05), significant congestive heart failure, or mitral regurgitation (P less than 0.01). Survival in multigrafted (and multivessel) patients was not significantly different from survival in single-grafted (predominantly single-vessel) patients. Actuarial studies suggest improved survival in patients with multivessel disease after coronary artery surgery. Between an initial evaluation at 9 months postoperatively (range: 2 to 40 months) and the most recent evaluation after 30 months (range: 6 to 72 months), 13% of patients showed further clinical improvement, 47% were unchanged, while 40% deteriorated with respect to chest pain. We conclude that initial symptomatic benefits may not be maintained in late follow-up studies owing to progression of underlying vascular disease."}
{"id": "PMID:1080448", "title": "Postoperative changes in aortocoronary saphenous vein grafts revisited: angiographic studies at two weeks and at one year in two series of consecutive patients.", "content": "Comparison of aortocoronary saphenous vein graft status at two weeks and at one year was made in two series of patients. The early postoperative evaluation includes 122 patients of the first 138 operated in our institution (182 grafts) and 83 subjects from a second series of 100 patients operated after modifications of surgical techniques (184 grafts). The one-year follow-up study was obtained in 105 patients of the first series (154 grafts) and in 67 of the second (152 grafts). The patency rate at two weeks was not significantly different between the two groups: 86.3% vx. 91.8%. A marked decrease of steonses noted early at anastomotic sites was observed: 15% to 5.5% (P less than 0.025). The patency rate at one year improved from 67% to 85,5% (P less than 0.0005). Grafts having flows at operation of at least 50 ml/min had patency rates of 90% in both series. The patency in grafts with initial flows below 50 ml/min increased from 28% to 73% (P less than 0.005). The incidence of late localized graft stenoses greater than 40% decreased from 16.5% to 6% (P less than 0.025), and diffuse narrowing greater than 40% was found in only 12% of the patent grafts at one year in the second series as compared to 31% in the first (P less than 0.001). These improved results do not appear to have been influenced by selection favoring better distal run-off in grafted arteries nor by the introduction of sequential grafts to multiple coronary arteries in the second group. Modified surgical techniques may explain the improved results.", "contents": "Postoperative changes in aortocoronary saphenous vein grafts revisited: angiographic studies at two weeks and at one year in two series of consecutive patients. Comparison of aortocoronary saphenous vein graft status at two weeks and at one year was made in two series of patients. The early postoperative evaluation includes 122 patients of the first 138 operated in our institution (182 grafts) and 83 subjects from a second series of 100 patients operated after modifications of surgical techniques (184 grafts). The one-year follow-up study was obtained in 105 patients of the first series (154 grafts) and in 67 of the second (152 grafts). The patency rate at two weeks was not significantly different between the two groups: 86.3% vx. 91.8%. A marked decrease of steonses noted early at anastomotic sites was observed: 15% to 5.5% (P less than 0.025). The patency rate at one year improved from 67% to 85,5% (P less than 0.0005). Grafts having flows at operation of at least 50 ml/min had patency rates of 90% in both series. The patency in grafts with initial flows below 50 ml/min increased from 28% to 73% (P less than 0.005). The incidence of late localized graft stenoses greater than 40% decreased from 16.5% to 6% (P less than 0.025), and diffuse narrowing greater than 40% was found in only 12% of the patent grafts at one year in the second series as compared to 31% in the first (P less than 0.001). These improved results do not appear to have been influenced by selection favoring better distal run-off in grafted arteries nor by the introduction of sequential grafts to multiple coronary arteries in the second group. Modified surgical techniques may explain the improved results."}
{"id": "PMID:1080449", "title": "Abnormal regional metabolism and mechanical function in patients with ischemic heart diseases: improvement after successful regional revascularization by aortocoronary bypass.", "content": "Left ventricular anterior wall metabolism was investigated concurrently with global myocardail metabolism by simultaneous preoperation sampling of anterior interventricular venous (AIV) and coronary sinus (CS) as well as arterial bloods in seven patients with severe obstructive lesions of the major coronary arteries, including left anterior descending. Postoperative study was performed two weeks to six months following successful aortocoronary artery bypass surgery. All grafts including the aorto-left anterior descending artery grafts were patent. Preoperatively in three of the seven patients, anterior wall lactate extraction (R%L) was negative at rest. The average R%L at rest (7 +/- 14%) was abnormal and was negative (-49 +/- 26%) at a maximum supraventricular pacing rate (MPR) of 137 +/- 4.6 beats/min. Postoperatively, not only was resting R%L (39 +/- 4;4%) normal but also it remained normal during atrial pacing (32 +/- 8.5%) even though the postoperative MPR (164 +/- 4.4 beats/min) was much higher than the preoperative MPR; Postoperatively AIV pO2 both at rest (21 +/- 1.1 mm Hg) and at MPR (22 +/- 1.3 mm Hg) and directly determined O2 saturations (resting: 34 +/- 3.0%; MPR:35 +/- 2.1%) tended to be higher than the preoperative values (AIV pO2, resting: 18 +/- 1.7; MPR: 19 +/- 1.7 mm Hg; AIV O2 saturation resting: 30 +/- 2.7; MPR: 33 +/- 3.3%), although only differences in pO2 were statistically significant. In five of the seven patients in whom the pre and postoperative left ventricular angiograms could be compared, systolic wall motion of the left ventricular anterior wall improved markedly postoperatively. Average global myocardial lactate extraction (G%L) preoperatively was normal (19 +/- 4.8%) at rest but was negative (-22 +/- 12%) at MPR. Postoperatively however, G%L both at rest (44 +/- 5.5%) and at MPR (34 +/- 7.9%) were normal. Coronary sinus pO2 and O2 saturation were also higher postoperatively compared to the preoperative values. Over-all left ventricular performance indicated by increase in ejection fraction also improved postoperatively. This improvement was not caused by increased coronary blood flow. Postoperative coronary sinus blood flow both at rest (114 +/- 19 ml/min) and at MPR (199 +/- 27 ml/min) however were less than the preoperative values (resting 136 +/- 24, MPR 261 +/- 40 ml/min), There was also no increase in global O2 delivery and O2 consumption despite higher heart rate and rate-pressure product achieved during postoperative pacing stress and the patients did not develop angina. These findings suggest that improved regional and global metabolism and mechanical functions observed postoperatively in these patients may be due to redistribution of blood flow to the ischemic and nonischemic myocardium following successful aortocoronary artery bypass surgery.", "contents": "Abnormal regional metabolism and mechanical function in patients with ischemic heart diseases: improvement after successful regional revascularization by aortocoronary bypass. Left ventricular anterior wall metabolism was investigated concurrently with global myocardail metabolism by simultaneous preoperation sampling of anterior interventricular venous (AIV) and coronary sinus (CS) as well as arterial bloods in seven patients with severe obstructive lesions of the major coronary arteries, including left anterior descending. Postoperative study was performed two weeks to six months following successful aortocoronary artery bypass surgery. All grafts including the aorto-left anterior descending artery grafts were patent. Preoperatively in three of the seven patients, anterior wall lactate extraction (R%L) was negative at rest. The average R%L at rest (7 +/- 14%) was abnormal and was negative (-49 +/- 26%) at a maximum supraventricular pacing rate (MPR) of 137 +/- 4.6 beats/min. Postoperatively, not only was resting R%L (39 +/- 4;4%) normal but also it remained normal during atrial pacing (32 +/- 8.5%) even though the postoperative MPR (164 +/- 4.4 beats/min) was much higher than the preoperative MPR; Postoperatively AIV pO2 both at rest (21 +/- 1.1 mm Hg) and at MPR (22 +/- 1.3 mm Hg) and directly determined O2 saturations (resting: 34 +/- 3.0%; MPR:35 +/- 2.1%) tended to be higher than the preoperative values (AIV pO2, resting: 18 +/- 1.7; MPR: 19 +/- 1.7 mm Hg; AIV O2 saturation resting: 30 +/- 2.7; MPR: 33 +/- 3.3%), although only differences in pO2 were statistically significant. In five of the seven patients in whom the pre and postoperative left ventricular angiograms could be compared, systolic wall motion of the left ventricular anterior wall improved markedly postoperatively. Average global myocardial lactate extraction (G%L) preoperatively was normal (19 +/- 4.8%) at rest but was negative (-22 +/- 12%) at MPR. Postoperatively however, G%L both at rest (44 +/- 5.5%) and at MPR (34 +/- 7.9%) were normal. Coronary sinus pO2 and O2 saturation were also higher postoperatively compared to the preoperative values. Over-all left ventricular performance indicated by increase in ejection fraction also improved postoperatively. This improvement was not caused by increased coronary blood flow. Postoperative coronary sinus blood flow both at rest (114 +/- 19 ml/min) and at MPR (199 +/- 27 ml/min) however were less than the preoperative values (resting 136 +/- 24, MPR 261 +/- 40 ml/min), There was also no increase in global O2 delivery and O2 consumption despite higher heart rate and rate-pressure product achieved during postoperative pacing stress and the patients did not develop angina. These findings suggest that improved regional and global metabolism and mechanical functions observed postoperatively in these patients may be due to redistribution of blood flow to the ischemic and nonischemic myocardium following successful aortocoronary artery bypass surgery."}
{"id": "PMID:1080450", "title": "Concordance for coronary risk factors among spouses.", "content": "Values for several coronary risk factors, including systolic and diastolic blood  pressure, serum cholesterol, triglycerides, blood glucose, uric acid, hemoglobin, weight, vitral capacity and cigarette smoking have been found to be similar among spouses in the Framingham Study. However, longitudinal analyses show that this spouse concordance does not increase over a twelve-year observation period, suggesting that it has arisen through the marriage of similar people rather than through the sharing of a common marital environment. Apparent conflicts between cross-sectional and longitudinal findings have been resolved by showing that spouses who were concordant at the begining of the study are more likely to survive to later exams, while discordant spouse pairs tend to be dissolved through the death of one of their members.", "contents": "Concordance for coronary risk factors among spouses. Values for several coronary risk factors, including systolic and diastolic blood  pressure, serum cholesterol, triglycerides, blood glucose, uric acid, hemoglobin, weight, vitral capacity and cigarette smoking have been found to be similar among spouses in the Framingham Study. However, longitudinal analyses show that this spouse concordance does not increase over a twelve-year observation period, suggesting that it has arisen through the marriage of similar people rather than through the sharing of a common marital environment. Apparent conflicts between cross-sectional and longitudinal findings have been resolved by showing that spouses who were concordant at the begining of the study are more likely to survive to later exams, while discordant spouse pairs tend to be dissolved through the death of one of their members."}
{"id": "PMID:1080453", "title": "The treatment of pulmonary metastasis in osteogenic sarcoma.", "content": "The survival times in a control series of 145 cases of osteogenic sarcoma without pulmonary resections including records of pulmonary nodules and surgical wedge excisions, suggest that pulmonary resection is a noteworthy adjunct treatment. The 5-year survival of patients subjected to repeated pulmonary surgery is known for 22 patients who were under the age of 21. Where aggressive pulmonary resections were performed, i.e., repeated thoracotomies, and multiple wedge resections of nodules, a 5-year survival rate of 31 per cent (after date of primary amputation) is noted and compared to a previous study of 145 cases, 121 of which had untreated pulmonary metastases, and at 5 years, only 2 per cent of these patients with metastases were still alive. Lung wedge resection survivals are highly significant statistically although survival with residual disease must be considered in part at least, due to aggressive chemotherapy (i.e., high dose methotrexate with citrovorum rescue in combination with other drugs).", "contents": "The treatment of pulmonary metastasis in osteogenic sarcoma. The survival times in a control series of 145 cases of osteogenic sarcoma without pulmonary resections including records of pulmonary nodules and surgical wedge excisions, suggest that pulmonary resection is a noteworthy adjunct treatment. The 5-year survival of patients subjected to repeated pulmonary surgery is known for 22 patients who were under the age of 21. Where aggressive pulmonary resections were performed, i.e., repeated thoracotomies, and multiple wedge resections of nodules, a 5-year survival rate of 31 per cent (after date of primary amputation) is noted and compared to a previous study of 145 cases, 121 of which had untreated pulmonary metastases, and at 5 years, only 2 per cent of these patients with metastases were still alive. Lung wedge resection survivals are highly significant statistically although survival with residual disease must be considered in part at least, due to aggressive chemotherapy (i.e., high dose methotrexate with citrovorum rescue in combination with other drugs)."}
{"id": "PMID:1080457", "title": "Dissection and rupture of the ascending aorta. Unusual complications of aortocoronary bypass surgery.", "content": "This is a report concerning two cases of unusual complications, dissection and rupture of the ascending aorta, secondary to saphenous vein bypass surgery. We recommend that evaluation of patients undergoing saphenous vein bypass surgery include careful image-intensifier fluoroscopic examination during coronary arteriography. If aneurysmal dilatation of the aorta or calcification is observed, ascending aortography should be performed to better evaluate the aorta, and surgeons should be alerted to use appropriate maneuvers and techniques to minimize the risk of complications in the aortic site of the vein anastomosis. Internal mammary coronary-artery bypass should be the procedure of choice in such conditions.", "contents": "Dissection and rupture of the ascending aorta. Unusual complications of aortocoronary bypass surgery. This is a report concerning two cases of unusual complications, dissection and rupture of the ascending aorta, secondary to saphenous vein bypass surgery. We recommend that evaluation of patients undergoing saphenous vein bypass surgery include careful image-intensifier fluoroscopic examination during coronary arteriography. If aneurysmal dilatation of the aorta or calcification is observed, ascending aortography should be performed to better evaluate the aorta, and surgeons should be alerted to use appropriate maneuvers and techniques to minimize the risk of complications in the aortic site of the vein anastomosis. Internal mammary coronary-artery bypass should be the procedure of choice in such conditions."}
{"id": "PMID:1080458", "title": "Saphenous vein graft from aorta to coronary vein with production of continuous murmur. A complication of coronary artery bypass surgery.", "content": "A saphenous vein graft was inadvertently placed from the aorta to the coronary vein adjacent to the proximal left anterior descending coronary artery in a 53-year-old man with symptomatic coronary artery disease. The post-operative finding of a continuous murmur led to cardiac catheterization and successful surgical correction. The postoperative finding of a continuous murmur must alert the clinician to this possible technical error.", "contents": "Saphenous vein graft from aorta to coronary vein with production of continuous murmur. A complication of coronary artery bypass surgery. A saphenous vein graft was inadvertently placed from the aorta to the coronary vein adjacent to the proximal left anterior descending coronary artery in a 53-year-old man with symptomatic coronary artery disease. The post-operative finding of a continuous murmur led to cardiac catheterization and successful surgical correction. The postoperative finding of a continuous murmur must alert the clinician to this possible technical error."}
{"id": "PMID:1080459", "title": "[Complications following colon surgery].", "content": "615 transperitoneal operations on the colon and rectum produced the following results. 377 abdominoperineal resections, anterior resections, partial and subtotal colectomies and proctocolectomies, 87 closures of colostomies and 151 operations with resection. 66% of the operations were required due to tumors. The death rate with 545 preopreratively prepared and scheduled operations was 8.1% whereas with the 70 emergency operations it was 25.7%. In 254 or 41.3% of the cases there were postoperative complications. Severe postoperative complications due to infection were the most frequent, followed by cardiopulmonary, haemorrhagic, urological and complications due to ileus. The quotient of insufficiency with the 356 anastomoses was 16%, the death rate 1.4%.", "contents": "[Complications following colon surgery]. 615 transperitoneal operations on the colon and rectum produced the following results. 377 abdominoperineal resections, anterior resections, partial and subtotal colectomies and proctocolectomies, 87 closures of colostomies and 151 operations with resection. 66% of the operations were required due to tumors. The death rate with 545 preopreratively prepared and scheduled operations was 8.1% whereas with the 70 emergency operations it was 25.7%. In 254 or 41.3% of the cases there were postoperative complications. Severe postoperative complications due to infection were the most frequent, followed by cardiopulmonary, haemorrhagic, urological and complications due to ileus. The quotient of insufficiency with the 356 anastomoses was 16%, the death rate 1.4%."}
{"id": "PMID:1080460", "title": "[Measurement of electric impedance as a screening method for diagnosing haemorrhagic diatheses (author's transl)].", "content": "Changes in electrical impedance during blood clotting have been previously described. Personal measurements on blood of 18 healthy donors and 29 patients with various disorders of clotting gave reproducible results with this method. There was a marked difference between normal subjects and those with bleeding diatheses regardless of type. There was good correlation between impedance changes and the thrombelastogram, the former being more sensitive in diagnosing haemorrhagic disorders. Further studies are required to evaluate its place as a screening method in these conditions.", "contents": "[Measurement of electric impedance as a screening method for diagnosing haemorrhagic diatheses (author's transl)]. Changes in electrical impedance during blood clotting have been previously described. Personal measurements on blood of 18 healthy donors and 29 patients with various disorders of clotting gave reproducible results with this method. There was a marked difference between normal subjects and those with bleeding diatheses regardless of type. There was good correlation between impedance changes and the thrombelastogram, the former being more sensitive in diagnosing haemorrhagic disorders. Further studies are required to evaluate its place as a screening method in these conditions."}
{"id": "PMID:1080461", "title": "[Early carcinoma of the stomach in Europe (author's transl)].", "content": "In a retrospective study of 317 816 gastroscopies between 1968 and 1973, the incidence of early carcinoma of the stomach was assessed. Among 18 887 gastric carcinomas there were 1170 early carcinomas. The most frequent kind was one of mucosal changes with defect (types II c and III according to the Japanese classification). 47% of early carcinomas were located in the lesser curvature. The diagnosis was made by biopsy in 90% of cases. The correctness of biopsy was proportional to the number of biopsies per lesion. Adding cytological examination to biopsy decreased the number of false-negative results by 30%. In most cases the history was non-specific. But in 70 patients (16%) over 40 years gastro-intestinal bleedings were the reason for endoscopic examination and led to the diagnosis of early carcinoma.", "contents": "[Early carcinoma of the stomach in Europe (author's transl)]. In a retrospective study of 317 816 gastroscopies between 1968 and 1973, the incidence of early carcinoma of the stomach was assessed. Among 18 887 gastric carcinomas there were 1170 early carcinomas. The most frequent kind was one of mucosal changes with defect (types II c and III according to the Japanese classification). 47% of early carcinomas were located in the lesser curvature. The diagnosis was made by biopsy in 90% of cases. The correctness of biopsy was proportional to the number of biopsies per lesion. Adding cytological examination to biopsy decreased the number of false-negative results by 30%. In most cases the history was non-specific. But in 70 patients (16%) over 40 years gastro-intestinal bleedings were the reason for endoscopic examination and led to the diagnosis of early carcinoma."}
{"id": "PMID:1080462", "title": "Primary immunodeficiency with early encephalopathy in two siblings. An anatomo-clinical study.", "content": "The authors describe two siblings affected by an immunodeficiency state characterized by low IgA and IgM levels in serum, and an early encephalopathy with infantile spasms and hypsarrhythmia. The nosography of this unusual association and the relationship to ataxia telangiectasia and allied disorders are briefly discussed.", "contents": "Primary immunodeficiency with early encephalopathy in two siblings. An anatomo-clinical study. The authors describe two siblings affected by an immunodeficiency state characterized by low IgA and IgM levels in serum, and an early encephalopathy with infantile spasms and hypsarrhythmia. The nosography of this unusual association and the relationship to ataxia telangiectasia and allied disorders are briefly discussed."}
{"id": "PMID:1080463", "title": "Myasthenia gravis: ALG treatment of seriously ill patients.", "content": "The authors review the theoretical basis of human antilymphocyte globulin (ALG) and human antihymocyte globulin (ATG) treatment in myasthenia gravis. Ten selected seriously ill myasthenic patients were treated with ALG and/or ATG. Three of the patients improved significantly and a further four moderately, whereas the condition of three patients did not change. ALG therapy is effective in the suppression of cellular immune mechanism, therefore it can be assumed that it exerts its effect in myasthenia gravis by inhibiting thymus hormone stimulation.", "contents": "Myasthenia gravis: ALG treatment of seriously ill patients. The authors review the theoretical basis of human antilymphocyte globulin (ALG) and human antihymocyte globulin (ATG) treatment in myasthenia gravis. Ten selected seriously ill myasthenic patients were treated with ALG and/or ATG. Three of the patients improved significantly and a further four moderately, whereas the condition of three patients did not change. ALG therapy is effective in the suppression of cellular immune mechanism, therefore it can be assumed that it exerts its effect in myasthenia gravis by inhibiting thymus hormone stimulation."}
{"id": "PMID:1080464", "title": "Stimulation of granulopoiesis by androgens without concomitant increase in the serum level of colony stimulating factor.", "content": "The marked enhancing effect of daily injections of 19-nortestosterone decanoate (19-ND) upon granulopoietic recovery of mice made neutropenic by a single dose of BCNU was studeid in regard to the possible regulatory role of CSF. Serum levels of CSF in BCNU-treated mice with or without 19-ND injection were not found to differ significantly from those in normal mice. BCNU treatment with or without 19-ND did not alter the CSF increases in response to endotoxin injection. Increases in dividing marrow granulocytes in the 19-ND mice were preceded by increases in their marrow CFC. The acceleration of granulopoietic regeneration was thus not mediated by the serum colony stimulating factor. It is attributed to a stimulatory effect of 19-ND on proliferation of CFC and/or CFU.", "contents": "Stimulation of granulopoiesis by androgens without concomitant increase in the serum level of colony stimulating factor. The marked enhancing effect of daily injections of 19-nortestosterone decanoate (19-ND) upon granulopoietic recovery of mice made neutropenic by a single dose of BCNU was studeid in regard to the possible regulatory role of CSF. Serum levels of CSF in BCNU-treated mice with or without 19-ND injection were not found to differ significantly from those in normal mice. BCNU treatment with or without 19-ND did not alter the CSF increases in response to endotoxin injection. Increases in dividing marrow granulocytes in the 19-ND mice were preceded by increases in their marrow CFC. The acceleration of granulopoietic regeneration was thus not mediated by the serum colony stimulating factor. It is attributed to a stimulatory effect of 19-ND on proliferation of CFC and/or CFU."}
{"id": "PMID:1080465", "title": "T and B lymphocytes in patients with chronic renal disease on hemodialysis.", "content": "The T and B lymphocytes in peripheral blood were reduced in patients with glomerulonephritis treated with hemodialysis as compared to normals. Although the absolute number of 'null' cells was also decreased, the percentage of 'null' cells was increased in these patients.", "contents": "T and B lymphocytes in patients with chronic renal disease on hemodialysis. The T and B lymphocytes in peripheral blood were reduced in patients with glomerulonephritis treated with hemodialysis as compared to normals. Although the absolute number of 'null' cells was also decreased, the percentage of 'null' cells was increased in these patients."}
{"id": "PMID:1080468", "title": "The role of the dentist in the emergency room.", "content": "Although most of the problems seen by the dentist in the hospital emergency room are not life-endangering, they can still cause considerable difficulty for the patient and anxiety for the doctor when not treated quickly and effectively. To manage an emergency effectively requires a prepared plan of action. Such planning must participation of the physicians, nurses, and auxiliary emergency room personnel. Since oral and maxillofacial emergencies may present unfamiliar problems to them, they must be instructed in advance about their role in proper care of such patients. Likewise, the dentist must familiarize himself with the emergency room- its operation and its facilities. Through such cooperative efforts, effective patient management will be provided no matter what unforeseen situation may suddenly arise.", "contents": "The role of the dentist in the emergency room. Although most of the problems seen by the dentist in the hospital emergency room are not life-endangering, they can still cause considerable difficulty for the patient and anxiety for the doctor when not treated quickly and effectively. To manage an emergency effectively requires a prepared plan of action. Such planning must participation of the physicians, nurses, and auxiliary emergency room personnel. Since oral and maxillofacial emergencies may present unfamiliar problems to them, they must be instructed in advance about their role in proper care of such patients. Likewise, the dentist must familiarize himself with the emergency room- its operation and its facilities. Through such cooperative efforts, effective patient management will be provided no matter what unforeseen situation may suddenly arise."}
{"id": "PMID:1080473", "title": "Ageing and autoimmunity.", "content": "A total of 483 healthy persons from Czechoslovakia, divided into three age groups (below 40, 41-60 and above 61 years) were examined for eight types of autoantibodies (antibodies to epithelial cells and to colloid of thyroid gland, parietal cell antibodies, antibody to intersititial duct cells of parotis and to adrenal cells, anti-nuclear and anti-mitochondrial antibodies, intrinsic factor antibodies). A significantly increased occurrence was observed in the oldest age group, the results being more manifest in women. A conspicious increase was seen particularly in the thyroid antibodies. The results correspond to those of other authors from different geographic areas.", "contents": "Ageing and autoimmunity. A total of 483 healthy persons from Czechoslovakia, divided into three age groups (below 40, 41-60 and above 61 years) were examined for eight types of autoantibodies (antibodies to epithelial cells and to colloid of thyroid gland, parietal cell antibodies, antibody to intersititial duct cells of parotis and to adrenal cells, anti-nuclear and anti-mitochondrial antibodies, intrinsic factor antibodies). A significantly increased occurrence was observed in the oldest age group, the results being more manifest in women. A conspicious increase was seen particularly in the thyroid antibodies. The results correspond to those of other authors from different geographic areas."}
{"id": "PMID:1080476", "title": "Human T-lymphocyte rosette formation: inhibition by cytochalasin B.", "content": "The effects of cytochalasin B, colchicine and vinblastine on the rosette formation of human T lymphocytes with neuraminidase-treated human erythrocytes (nHRBC) and with sheep red blood cells (SRBC) have been studied. Pretreatment of the lymphocytes with cytochalasin B which affects microfilament action reversibly inhibits both nHRBC and SRBC rosette formation. Colchicine and vinblastine known to interact with microtubules causes no major reduction in rosette-forming cells. The results suggest that normally functioning microfilaments are necessary for nHRBC and SRBC rosetting, whereas microtubules are not essential for the blinding of the erythrocytes to the lymphocytes.", "contents": "Human T-lymphocyte rosette formation: inhibition by cytochalasin B. The effects of cytochalasin B, colchicine and vinblastine on the rosette formation of human T lymphocytes with neuraminidase-treated human erythrocytes (nHRBC) and with sheep red blood cells (SRBC) have been studied. Pretreatment of the lymphocytes with cytochalasin B which affects microfilament action reversibly inhibits both nHRBC and SRBC rosette formation. Colchicine and vinblastine known to interact with microtubules causes no major reduction in rosette-forming cells. The results suggest that normally functioning microfilaments are necessary for nHRBC and SRBC rosetting, whereas microtubules are not essential for the blinding of the erythrocytes to the lymphocytes."}
{"id": "PMID:1080480", "title": "Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells.", "content": "Studies were performed to characterize the effector cells responsible for natural cytotoxicity of mouse lymphoid cells against a variety of syngeneic and allogeneic tumor lines. Since spleen cells from normal nude mice were found to be highly cytotoxic, they were used for most of these experiments. Only a small proportion of the reactivity was affected by treatment with anti-theta serum plus complement. Macrophages dis not appear to be responsible for the reactivity, since treatment with carbonyl iron/magnet or carrageenan did not affect the levels of cytotoxicity. The effector cells were non-adherent, since passage over nylon columns resulted in a considerable increase in activity. The active cells did not have receptors for immunoglobulin or complement, since removal of cells with these receptors by columns or monolayers containing sheep erythrocyte-antibody (EA) complexes or EA-complement complexes did not remove activity. Antibody-dependent cell-mediated cytotoxicity appeared to be ruled out as the mechanism for natural cytotoxicity, since aggregated gamma globulin and a potent anti-immunoglobulin reagent did not inhibit reactivity, and since no role for humoral factors could be demonstrated. The natural effector cell was found to be quite labile at 37 degrees C, losing much of its activity after 4 h. Since no surface markers could be detected on the effector cells, and the mechanism for cytotoxicity appeared distince from others previously described, it is proposed that the natural cytotoxicity against mouse tumor cells is mediated by a unique subpopulation of lymphoid cells, which are tentatively designated N-cells.", "contents": "Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. Studies were performed to characterize the effector cells responsible for natural cytotoxicity of mouse lymphoid cells against a variety of syngeneic and allogeneic tumor lines. Since spleen cells from normal nude mice were found to be highly cytotoxic, they were used for most of these experiments. Only a small proportion of the reactivity was affected by treatment with anti-theta serum plus complement. Macrophages dis not appear to be responsible for the reactivity, since treatment with carbonyl iron/magnet or carrageenan did not affect the levels of cytotoxicity. The effector cells were non-adherent, since passage over nylon columns resulted in a considerable increase in activity. The active cells did not have receptors for immunoglobulin or complement, since removal of cells with these receptors by columns or monolayers containing sheep erythrocyte-antibody (EA) complexes or EA-complement complexes did not remove activity. Antibody-dependent cell-mediated cytotoxicity appeared to be ruled out as the mechanism for natural cytotoxicity, since aggregated gamma globulin and a potent anti-immunoglobulin reagent did not inhibit reactivity, and since no role for humoral factors could be demonstrated. The natural effector cell was found to be quite labile at 37 degrees C, losing much of its activity after 4 h. Since no surface markers could be detected on the effector cells, and the mechanism for cytotoxicity appeared distince from others previously described, it is proposed that the natural cytotoxicity against mouse tumor cells is mediated by a unique subpopulation of lymphoid cells, which are tentatively designated N-cells."}
{"id": "PMID:1080485", "title": "Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation.", "content": "Cell envelopes of Haemophilus influenzae have been prepared by breakage in a French pressure cell followed by differential centrifugation. The envelope fraction may be resolved into an inner-membrane (light) and an outer-membrane (heavy) fraction on density gradients. Envelopes from competent cells possess elevated levels of lipopolysaccharide with a composition different from that of log-phase cell envelopes. Three apparently new polypeptides have been observed in envelopes from competent cells by gel electrophoresis in sodium dodecyl sulfate; additional quantitative alterations in the profiles of membrane polypeptides also company the development of the capacity to transport deoxyribonucleic acid. Most of the polypeptide changes are confined to the outer membrane; one new polypeptide is associated with the inner cytoplasmic membrane of competent cells. Protein synthesis during competence developement is rquired for the change in lipopolysaccharides and in the envelope polypeptides to occur.", "contents": "Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation. Cell envelopes of Haemophilus influenzae have been prepared by breakage in a French pressure cell followed by differential centrifugation. The envelope fraction may be resolved into an inner-membrane (light) and an outer-membrane (heavy) fraction on density gradients. Envelopes from competent cells possess elevated levels of lipopolysaccharide with a composition different from that of log-phase cell envelopes. Three apparently new polypeptides have been observed in envelopes from competent cells by gel electrophoresis in sodium dodecyl sulfate; additional quantitative alterations in the profiles of membrane polypeptides also company the development of the capacity to transport deoxyribonucleic acid. Most of the polypeptide changes are confined to the outer membrane; one new polypeptide is associated with the inner cytoplasmic membrane of competent cells. Protein synthesis during competence developement is rquired for the change in lipopolysaccharides and in the envelope polypeptides to occur."}
{"id": "PMID:1080486", "title": "Minicell production and bacteriophage superinducibility of thymidine-requiring strains of Haemophilus influenzae.", "content": "Aminopterin- or trimethoprin-resistant thymidine-requiring strains of Haemophilus influenzae produce minicells, and the ratio of minicells to cells increases during the stationary phase of growth. Strain LB11, isolated after mutagenesis of a thymidine-requiring strain (Rd thd), produces more minicells than the parent strain. The mutations involved in high frequency minicell production have been transferred into the wild type (strain Rd) by transformation. The thymidine requirement in the resulting strain, MCl, is essential for minicell production, since spontaneous revertants of MCl to prototrophy do not produce minicells. The ratio of minicells to cells was increased more than 10(3)-fold by differential centrifugation. The minicells contain little or no deoxyribonucleic acid (DNA). Phage HPlcl apparently cannot attach to minicells. Competent cells of LB11 and its thymidine-requiring parent strain produce defective phage as a result of exposure to transforming DNA, whereas only LB11 produces many defective phage in response to the competence regime alone. Competent HP1c1 and S2 lysogens of MC1 and Rd thd are also superinducible by transforming DNA, but competent LB11 lysogens produced about the same amount of HP1c1 or S2 phage with or without exposure to transforming DNA possibly because of competition between the induced defective phage and Hp1c1 or S2 phage.", "contents": "Minicell production and bacteriophage superinducibility of thymidine-requiring strains of Haemophilus influenzae. Aminopterin- or trimethoprin-resistant thymidine-requiring strains of Haemophilus influenzae produce minicells, and the ratio of minicells to cells increases during the stationary phase of growth. Strain LB11, isolated after mutagenesis of a thymidine-requiring strain (Rd thd), produces more minicells than the parent strain. The mutations involved in high frequency minicell production have been transferred into the wild type (strain Rd) by transformation. The thymidine requirement in the resulting strain, MCl, is essential for minicell production, since spontaneous revertants of MCl to prototrophy do not produce minicells. The ratio of minicells to cells was increased more than 10(3)-fold by differential centrifugation. The minicells contain little or no deoxyribonucleic acid (DNA). Phage HPlcl apparently cannot attach to minicells. Competent cells of LB11 and its thymidine-requiring parent strain produce defective phage as a result of exposure to transforming DNA, whereas only LB11 produces many defective phage in response to the competence regime alone. Competent HP1c1 and S2 lysogens of MC1 and Rd thd are also superinducible by transforming DNA, but competent LB11 lysogens produced about the same amount of HP1c1 or S2 phage with or without exposure to transforming DNA possibly because of competition between the induced defective phage and Hp1c1 or S2 phage."}
{"id": "PMID:1080488", "title": "Lack of T-cell immune abnormalities in peripheral blood lymphocytes in patients with Graves' disease or hypothyroidism.", "content": "Lymphocytes capable of forming nonimmune (E) and immune (EAC) rosettes with sheep erythrocytes and lymphocyte reactivity to phytohemagglutinin were determined in normal subjects, in patients with hyperthyroidism due to Graves' disease and in patients with hypothyroidism with and without goiter. Neither patient group differed from the normal subjects either in regard to lymphocyte count, the proportion of thymus dependent (t) lymphocytes forming nonimmune or bone marrow-dependnet (B) lymphocytes forming immune rosettes or lymphocyte reactivity to phytohemagglutinin. These results fail to provide evidence that these disorders are associated with an immune abnormality with increased or abnormal T cells in peripheral blood.", "contents": "Lack of T-cell immune abnormalities in peripheral blood lymphocytes in patients with Graves' disease or hypothyroidism. Lymphocytes capable of forming nonimmune (E) and immune (EAC) rosettes with sheep erythrocytes and lymphocyte reactivity to phytohemagglutinin were determined in normal subjects, in patients with hyperthyroidism due to Graves' disease and in patients with hypothyroidism with and without goiter. Neither patient group differed from the normal subjects either in regard to lymphocyte count, the proportion of thymus dependent (t) lymphocytes forming nonimmune or bone marrow-dependnet (B) lymphocytes forming immune rosettes or lymphocyte reactivity to phytohemagglutinin. These results fail to provide evidence that these disorders are associated with an immune abnormality with increased or abnormal T cells in peripheral blood."}
{"id": "PMID:1080489", "title": "Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus.", "content": "Although the association of cryoglobulinemia with hypocomplementemia and tissue injury in systemic lupus erythematosus is well recognized, composition of cryoprecipitates in terms of circulating antigens and antibodies in this disease is less clear. To clarify this question, cryoprecipitates from patients with SLE were examined with sensitive assay techniques for certain antipolynucleotide antibodies and DNA antigen. DNA antibodies were highly enriched relative to serum levels in the majority of cryoprecipitates. DNA antigen was also demonstrable. Antibody to ribonucleoprotein, although less frequently present, was similarly enriched in certain cryoprecipitates. In contrast, anti-double strand RNA, which was commonly detectable in relatively high titer in serum, was only minimally concentrated in a minority of cryoprecipitates. Absorption experiments using red blood cells heavily coated with polynucleotide antigen indicated that a major proportion of the IgG in certain cryoprecipitates was specific antibody. The data strongly suggest that the cryoprecipitates in systemic lupus erythematosus represent circulating immune complexes that are soluble at 37 degrees C and come out of solution in the cold. The marked concentration of immune complexes in the cryoglobulin offers a simple and direct method for determination of the nature of the complexes. The accumulated evidence obtained in the present study indicates that these complexes closely reflect, in their composition, the circulating immune complexes which are most significant pathogenetically in renal tissue injury.", "contents": "Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus. Although the association of cryoglobulinemia with hypocomplementemia and tissue injury in systemic lupus erythematosus is well recognized, composition of cryoprecipitates in terms of circulating antigens and antibodies in this disease is less clear. To clarify this question, cryoprecipitates from patients with SLE were examined with sensitive assay techniques for certain antipolynucleotide antibodies and DNA antigen. DNA antibodies were highly enriched relative to serum levels in the majority of cryoprecipitates. DNA antigen was also demonstrable. Antibody to ribonucleoprotein, although less frequently present, was similarly enriched in certain cryoprecipitates. In contrast, anti-double strand RNA, which was commonly detectable in relatively high titer in serum, was only minimally concentrated in a minority of cryoprecipitates. Absorption experiments using red blood cells heavily coated with polynucleotide antigen indicated that a major proportion of the IgG in certain cryoprecipitates was specific antibody. The data strongly suggest that the cryoprecipitates in systemic lupus erythematosus represent circulating immune complexes that are soluble at 37 degrees C and come out of solution in the cold. The marked concentration of immune complexes in the cryoglobulin offers a simple and direct method for determination of the nature of the complexes. The accumulated evidence obtained in the present study indicates that these complexes closely reflect, in their composition, the circulating immune complexes which are most significant pathogenetically in renal tissue injury."}
{"id": "PMID:1080490", "title": "Effects of thrombin treatment of preparations of factor VIII and the Ca2+-dissociated small active fragment.", "content": "When human, canine, or bovine factor VIII preparations are chromatographed on 4% agarose at ionic strength 0.2, the factor VIII activity elutes as a single peak in the void volume with slight tailing. Incubation of such preparations with dilute (0.01 U/ml) highly purified thrombin results in some activation of factor VIII. Chromatography of such incubation mixtures, under the same conditions as before, results in elution of two peaks of factor VIII activity one in the void volume and one much later with marked tailing. The void volume peak has most of the protein and some factor VIII activity. These void volume fractions also contain all the von Willebrand factor activity of thrombin-treated bovine preparations. Longer treatment with thrombin, or treatment with stronger thrombin, appears to shift much more of the procoagulant activity to the later eluting peak. Also, when the peak of factor VIII activity, found in the void volume after thrombin treatment, was again incubated with dilute thrombin, an increase in factor VIII activity occurred. Chromatography of this incubation mixture demonstrated only a small amount of activity in the void volume, while the bulk of the activity was present in the second peak. On the other hand, thrombin treatment of factor VIII activity from peak 2 caused a rapid decline of activity instead of a further increase. It is proposed that the residual factor VIII activity found in the void volume represents unreacted factor VIII, while the late eluting peak represents thrombin-activated material that is of smaller apparent size. The late eluting peak differs from the small active factor VIII fragment obtained by Ca2+ dissociation, as the latter can be activated by thrombin. A similar set of experiments was performed using ultracentifugation of bovine factor VIII preparations on sucrose density gradients. Results of these experiments agreed completely with those obtained with get chromatography. Preparations made from human hemophilic plasma, by the procudure employed in the purification of human factor VIII, were also incubated with thrombin and chromatographed. von Willebrand factor was again found only in the void volume fractions, but there was no factor VIII activity in any fractions eluted. In other control experiments, activated and unactivated factor VIII fractions did not clot fibrinogen and contained no assayable factor IX or X. The thrombin-modified factor VIII of small size was inactivated by both a naturally occurring human inhibitor to factor VIII and the gamma globulin fraction of a rabbit antisera produced against the calcium-dissociated small active factor VIII fragment.", "contents": "Effects of thrombin treatment of preparations of factor VIII and the Ca2+-dissociated small active fragment. When human, canine, or bovine factor VIII preparations are chromatographed on 4% agarose at ionic strength 0.2, the factor VIII activity elutes as a single peak in the void volume with slight tailing. Incubation of such preparations with dilute (0.01 U/ml) highly purified thrombin results in some activation of factor VIII. Chromatography of such incubation mixtures, under the same conditions as before, results in elution of two peaks of factor VIII activity one in the void volume and one much later with marked tailing. The void volume peak has most of the protein and some factor VIII activity. These void volume fractions also contain all the von Willebrand factor activity of thrombin-treated bovine preparations. Longer treatment with thrombin, or treatment with stronger thrombin, appears to shift much more of the procoagulant activity to the later eluting peak. Also, when the peak of factor VIII activity, found in the void volume after thrombin treatment, was again incubated with dilute thrombin, an increase in factor VIII activity occurred. Chromatography of this incubation mixture demonstrated only a small amount of activity in the void volume, while the bulk of the activity was present in the second peak. On the other hand, thrombin treatment of factor VIII activity from peak 2 caused a rapid decline of activity instead of a further increase. It is proposed that the residual factor VIII activity found in the void volume represents unreacted factor VIII, while the late eluting peak represents thrombin-activated material that is of smaller apparent size. The late eluting peak differs from the small active factor VIII fragment obtained by Ca2+ dissociation, as the latter can be activated by thrombin. A similar set of experiments was performed using ultracentifugation of bovine factor VIII preparations on sucrose density gradients. Results of these experiments agreed completely with those obtained with get chromatography. Preparations made from human hemophilic plasma, by the procudure employed in the purification of human factor VIII, were also incubated with thrombin and chromatographed. von Willebrand factor was again found only in the void volume fractions, but there was no factor VIII activity in any fractions eluted. In other control experiments, activated and unactivated factor VIII fractions did not clot fibrinogen and contained no assayable factor IX or X. The thrombin-modified factor VIII of small size was inactivated by both a naturally occurring human inhibitor to factor VIII and the gamma globulin fraction of a rabbit antisera produced against the calcium-dissociated small active factor VIII fragment."}
{"id": "PMID:1080491", "title": "Studies of the human factor VIII/von Willebrand factor protein. III. Qualitative defects in von Willebrand's disease.", "content": "The Factor VIII/von Willebrand factor protein was characterized in two unrelated patients with von Willebrand's disease in whom procoagulant and Factor VIII/von Willebrand factor antigen levels were normal. In both patients evidence of an abnormal protein was observed on crossed antigen-antibody electrophoresis. In one patient the Factor VIII/von Willebrand factor protein eluted from Sepharose 4B in a position and distribution identical to normal with normal levels of procoagulant activity and antigen. However, the partially purified Factor VIII/von Willebrand factor protein had markedly reduced von Willebrand factor activity in a ristocetin assay. In the second patient the peak of Factor VIII/von Willebrand factor protein, antigen, and procoagulant activity eluted from a Sepharose 4B column with an estimated molecular weight of approximately half that of normal. This protein had no von Willebrand factor activity. In both patients the reduced Factor VIII/von Willebrand factor protein subunit was indistinguishable from normal on polyacrylamide gel electrophoresis. These studies indicate that in some patients with von Willebrand's disease there is a qualitative defect of the Factor VII/von Willebrand factor protein; the total amount of protein, antigen, and procoagulant activity are normal while the von Willebrand factor activity is deficient.", "contents": "Studies of the human factor VIII/von Willebrand factor protein. III. Qualitative defects in von Willebrand's disease. The Factor VIII/von Willebrand factor protein was characterized in two unrelated patients with von Willebrand's disease in whom procoagulant and Factor VIII/von Willebrand factor antigen levels were normal. In both patients evidence of an abnormal protein was observed on crossed antigen-antibody electrophoresis. In one patient the Factor VIII/von Willebrand factor protein eluted from Sepharose 4B in a position and distribution identical to normal with normal levels of procoagulant activity and antigen. However, the partially purified Factor VIII/von Willebrand factor protein had markedly reduced von Willebrand factor activity in a ristocetin assay. In the second patient the peak of Factor VIII/von Willebrand factor protein, antigen, and procoagulant activity eluted from a Sepharose 4B column with an estimated molecular weight of approximately half that of normal. This protein had no von Willebrand factor activity. In both patients the reduced Factor VIII/von Willebrand factor protein subunit was indistinguishable from normal on polyacrylamide gel electrophoresis. These studies indicate that in some patients with von Willebrand's disease there is a qualitative defect of the Factor VII/von Willebrand factor protein; the total amount of protein, antigen, and procoagulant activity are normal while the von Willebrand factor activity is deficient."}
{"id": "PMID:1080493", "title": "Treatment goals in geropsychiatry.", "content": "Each geriatric patient has observable problems which can generate treatment goals implemented by a treatment plan. Extensive record keeping would be required to document this tripartite scheme. In practice, either problem-oriented or goal-oriented records are used. Problem records tend to drift toward a goal concept, since problems are usually stated in only sufficient detail to serve as a guide to treatment. The results of a problem-oriented format are described for 143 state hospital patients whose mean age was 74 years. The most common number of problems was 5 per patient. Physical problems were the most frequent (N equals 456), followed by behavioral (N equals 203), thought-related (N equals 156), administrative (N equals 99), affective (N equals 69), and attitudinal (N equals 10). The author's experience with both systems leaves them with preference for the problem-oriented system with geriatric patients since physical problems are so numerous.", "contents": "Treatment goals in geropsychiatry. Each geriatric patient has observable problems which can generate treatment goals implemented by a treatment plan. Extensive record keeping would be required to document this tripartite scheme. In practice, either problem-oriented or goal-oriented records are used. Problem records tend to drift toward a goal concept, since problems are usually stated in only sufficient detail to serve as a guide to treatment. The results of a problem-oriented format are described for 143 state hospital patients whose mean age was 74 years. The most common number of problems was 5 per patient. Physical problems were the most frequent (N equals 456), followed by behavioral (N equals 203), thought-related (N equals 156), administrative (N equals 99), affective (N equals 69), and attitudinal (N equals 10). The author's experience with both systems leaves them with preference for the problem-oriented system with geriatric patients since physical problems are so numerous."}
{"id": "PMID:1080492", "title": "Immunocompetent cells from the lower respiratory tract of normal human lungs.", "content": "Subpopulations of lymphocytes in the broncho-alveolar air spaces of normal human lungs were compared with those in peripheral blood. Bone marrow-derived (bursal-equivalent) cells (B cells) were identified by complement receptors (EAC rosettes) and by surface immunoglobulin. Thymus-derived lymphocytes (T cells) were identified by their proliferative response to mitogens and the E rosette technique. Cells in lung air spaces were recovered from eight healthy nonsmoking volunteers by segmental lavage with the flexible bronchofiberscope. On the average, macrophages constituted 78% and lymphocytes 17% of the cells in the aspirates. B cells detected by surface immunoglobulin and complement receptors equaled 22% and 15% of lung lymphocytes, respectively. The distribution of lung B cells into heavy chain immunoglobulin classes revealed IgM and IgG to be the predominant classes, with mean values of 14.5% and 9.3%, respectively; the corresponding value for IgA was 5%. A comparable order of frequency (IgM greater than IgG greater than IgA) was observed for purified peripheral blood lymphocytes in the same and other control subjects. T cells comprised the majority (47%) of identifiable lung lymphocytes by the E rosette method. The presence of lung T cells was also corroborated by their proliferative response to mitogens (phytohemagglutinin and concanavallin A), but the response was less than that of equal numbers of peripheral blood lymphocytes from the same subjects. The B/T cell ratio for lung lymphocytes was comparable to results with peripheral blood lymphocytes in the same subjects, but a higher proportion of lung lymphocytes could not be identified as either T or B cells. It is postulated that lung lymphocytes participate in the local immune defenses of the lung.", "contents": "Immunocompetent cells from the lower respiratory tract of normal human lungs. Subpopulations of lymphocytes in the broncho-alveolar air spaces of normal human lungs were compared with those in peripheral blood. Bone marrow-derived (bursal-equivalent) cells (B cells) were identified by complement receptors (EAC rosettes) and by surface immunoglobulin. Thymus-derived lymphocytes (T cells) were identified by their proliferative response to mitogens and the E rosette technique. Cells in lung air spaces were recovered from eight healthy nonsmoking volunteers by segmental lavage with the flexible bronchofiberscope. On the average, macrophages constituted 78% and lymphocytes 17% of the cells in the aspirates. B cells detected by surface immunoglobulin and complement receptors equaled 22% and 15% of lung lymphocytes, respectively. The distribution of lung B cells into heavy chain immunoglobulin classes revealed IgM and IgG to be the predominant classes, with mean values of 14.5% and 9.3%, respectively; the corresponding value for IgA was 5%. A comparable order of frequency (IgM greater than IgG greater than IgA) was observed for purified peripheral blood lymphocytes in the same and other control subjects. T cells comprised the majority (47%) of identifiable lung lymphocytes by the E rosette method. The presence of lung T cells was also corroborated by their proliferative response to mitogens (phytohemagglutinin and concanavallin A), but the response was less than that of equal numbers of peripheral blood lymphocytes from the same subjects. The B/T cell ratio for lung lymphocytes was comparable to results with peripheral blood lymphocytes in the same subjects, but a higher proportion of lung lymphocytes could not be identified as either T or B cells. It is postulated that lung lymphocytes participate in the local immune defenses of the lung."}
{"id": "PMID:1080495", "title": "Transfer of ampicillin resistance between strains of Haemophilus influenzae type B.", "content": "Haemophilus influenzae type B, strain W-2, is highly resistant to ampicillin (MIC, 12.5 mug/ml). The ampicillin resistance of strain W-2 was transferred to an antibiotic-sensitive strain TF-2 (RifR, SmR) during mixed incubation on membrane filters at 36 C(transfeer frequency, 4.6 times 10(-5) per donor). Resistance was also transferred from the primary recipient to a secondary one (TF-3, EryR, NovR). The transfer frequency between these derivative strains was 10(-4) after incubation for 30 min. Resistance in strain W-2 remained even after growth in the presence of ethidium bromide or at an elevated temperature, although ampicillin resistance was lost from 13%-25% of transcipient cells after growth in broth. Strain W-2 and transcipients of ampicillin resistance had equivalent levels of beta-lactamase activity, while sensitive segregants and recipient strains demonstrated little or no enzyme activity. Transfer of ampicillin resistance between strains of H. influenzae is probably mediated by conjugation since transfer (1) requires cell-to-cell contact, (2) remains unchanged in the presence of DNase I, and (3) occurs in the absence of demonstrable bacteriophage.", "contents": "Transfer of ampicillin resistance between strains of Haemophilus influenzae type B. Haemophilus influenzae type B, strain W-2, is highly resistant to ampicillin (MIC, 12.5 mug/ml). The ampicillin resistance of strain W-2 was transferred to an antibiotic-sensitive strain TF-2 (RifR, SmR) during mixed incubation on membrane filters at 36 C(transfeer frequency, 4.6 times 10(-5) per donor). Resistance was also transferred from the primary recipient to a secondary one (TF-3, EryR, NovR). The transfer frequency between these derivative strains was 10(-4) after incubation for 30 min. Resistance in strain W-2 remained even after growth in the presence of ethidium bromide or at an elevated temperature, although ampicillin resistance was lost from 13%-25% of transcipient cells after growth in broth. Strain W-2 and transcipients of ampicillin resistance had equivalent levels of beta-lactamase activity, while sensitive segregants and recipient strains demonstrated little or no enzyme activity. Transfer of ampicillin resistance between strains of H. influenzae is probably mediated by conjugation since transfer (1) requires cell-to-cell contact, (2) remains unchanged in the presence of DNase I, and (3) occurs in the absence of demonstrable bacteriophage."}
{"id": "PMID:1080496", "title": "Determination of the von Willebrand's disease antigen (factor VIII-related antigen) in plasma by quantitative immunoelectrophoresis.", "content": "Measurement of the von Willebrand's disease antigen (factor viii-related antigen) has become an increasingly useful tool in the differential diagnosis of those disorders of hemostasis characterized by an abnormality of factor viii procoagulant activity.  Optimal conditions for assay of the von Willebrand's disease antigen in plasma by the quantitive immunoelectrophoretic technique are described.  Potential sources ofsystematic error have been evaluated and a dilution effect has been identified.", "contents": "Determination of the von Willebrand's disease antigen (factor VIII-related antigen) in plasma by quantitative immunoelectrophoresis. Measurement of the von Willebrand's disease antigen (factor viii-related antigen) has become an increasingly useful tool in the differential diagnosis of those disorders of hemostasis characterized by an abnormality of factor viii procoagulant activity.  Optimal conditions for assay of the von Willebrand's disease antigen in plasma by the quantitive immunoelectrophoretic technique are described.  Potential sources ofsystematic error have been evaluated and a dilution effect has been identified."}
{"id": "PMID:1080514", "title": "Clinical results with metrizamide ventriculography.", "content": "Seventeen ventriculograms with the non-ionic water-soluble contrast medium, metrizamide, were performed in 15 patients, with neglibible adverse effects. Visualization of the ventricular system was good in most of the patients. A combined technique, using both metrizamide and gas, increased the diagnostic value of the procedure in some cases.", "contents": "Clinical results with metrizamide ventriculography. Seventeen ventriculograms with the non-ionic water-soluble contrast medium, metrizamide, were performed in 15 patients, with neglibible adverse effects. Visualization of the ventricular system was good in most of the patients. A combined technique, using both metrizamide and gas, increased the diagnostic value of the procedure in some cases."}
{"id": "PMID:1080515", "title": "Percutaneous trial of stimulation for patient selection for implantable stimulating devices.", "content": "The author describes a flexible electrode which can be inserted percutaneously for a period of several days in candidates for an implantable electrical stimulating device for pain relief. This allows the patient a trial of stimulation which closely mimics that of the intended implantable system. If this trial does not give adequate pain relief in a variety of situations, the patient is not considered to be a suitable candidate for an implantable device. The trial of stimulation in no way obviates the need for careful scrutiny of the social and psychological factors accompanying chronic pain problems.", "contents": "Percutaneous trial of stimulation for patient selection for implantable stimulating devices. The author describes a flexible electrode which can be inserted percutaneously for a period of several days in candidates for an implantable electrical stimulating device for pain relief. This allows the patient a trial of stimulation which closely mimics that of the intended implantable system. If this trial does not give adequate pain relief in a variety of situations, the patient is not considered to be a suitable candidate for an implantable device. The trial of stimulation in no way obviates the need for careful scrutiny of the social and psychological factors accompanying chronic pain problems."}
{"id": "PMID:1080516", "title": "Familial variable immunodeficiency: autosomal dominant pattern of inheritance with variable expression of the defect(s).", "content": "In 1963, Rosen and Bougas reported the case of a woman with recurrent infection, marked elevation of 19S, and virtual absence of 7S gamma globulin. Recently, members of her family were found to have similar abnormalities: Ten of the 37 family members tested had elevated levels of serum IgM accompanied by a combined deficiency of IgG and IgA in three, and by a deficiency of either IgG or IgA in two. In five, an increase in IgM was the sole abnormality. Two children had deficiencies of IgG and IgA with normal serum levels of IgM. Ten of the 12 affected individuals had no IgD detectable by radial immunodiffusion and six had a low percentage of IgG-bearing B lymphocytes. A lack of correlation between the immunochemical abnormalities and either the presence or severity of clinical illness was observed. The presence of immunodeficiency in three generations and in both sexes of this family suggests an autosomal dominant mode of inheritance with variable penetrance of the defect.", "contents": "Familial variable immunodeficiency: autosomal dominant pattern of inheritance with variable expression of the defect(s). In 1963, Rosen and Bougas reported the case of a woman with recurrent infection, marked elevation of 19S, and virtual absence of 7S gamma globulin. Recently, members of her family were found to have similar abnormalities: Ten of the 37 family members tested had elevated levels of serum IgM accompanied by a combined deficiency of IgG and IgA in three, and by a deficiency of either IgG or IgA in two. In five, an increase in IgM was the sole abnormality. Two children had deficiencies of IgG and IgA with normal serum levels of IgM. Ten of the 12 affected individuals had no IgD detectable by radial immunodiffusion and six had a low percentage of IgG-bearing B lymphocytes. A lack of correlation between the immunochemical abnormalities and either the presence or severity of clinical illness was observed. The presence of immunodeficiency in three generations and in both sexes of this family suggests an autosomal dominant mode of inheritance with variable penetrance of the defect."}
{"id": "PMID:1080518", "title": "Teaching problem-oriented recording: a systematic approach.", "content": "Medical students and practitioners usually learn how to use problem-oriented medical records (POMR) by haphazard means. In attempting to put system into the teaching of POMR, the authors devised teaching case material and two instructional formats: self-instruction and workshop. The results of a controlled study with second-year medical students to determine the relative effectiveness of instructional formats and case materials are presented. At the end of instruction POMR performance was measured by assessing the student's ability to convert a case to POMR format. The results suggest that all student groups attained an acceptable performance level no matter which format they experienced.", "contents": "Teaching problem-oriented recording: a systematic approach. Medical students and practitioners usually learn how to use problem-oriented medical records (POMR) by haphazard means. In attempting to put system into the teaching of POMR, the authors devised teaching case material and two instructional formats: self-instruction and workshop. The results of a controlled study with second-year medical students to determine the relative effectiveness of instructional formats and case materials are presented. At the end of instruction POMR performance was measured by assessing the student's ability to convert a case to POMR format. The results suggest that all student groups attained an acceptable performance level no matter which format they experienced."}
{"id": "PMID:1080519", "title": "The nonhistone chromosomal proteins of vertebrate liver and kidney: a comparative study by gel electrophoresis.", "content": "The nonhistone chromosomal proteins (NHC proteins) probably include enzymes of chromosomal metabolism, general structural proteins, and possibly control elements. In theory, these proteins may have been strongly conserved during evolution, as the histones have. We have used sodium dodecyl sulfate (SDS) disc gel electrophoresis to analyze and compare the NHC proteins of two tissues, liver and kidney, from rat, cat, cow, chicken, turtle, and frog. The gel patterns indicate that the NHC proteins have changed much more during evolution than have the histones; the total pattern of NHC proteins has not been conserved. However, there does appear to be a conservation of a subset of bands for each tissue investigated. Further chemical analysis will be required to establish the significance of the results.", "contents": "The nonhistone chromosomal proteins of vertebrate liver and kidney: a comparative study by gel electrophoresis. The nonhistone chromosomal proteins (NHC proteins) probably include enzymes of chromosomal metabolism, general structural proteins, and possibly control elements. In theory, these proteins may have been strongly conserved during evolution, as the histones have. We have used sodium dodecyl sulfate (SDS) disc gel electrophoresis to analyze and compare the NHC proteins of two tissues, liver and kidney, from rat, cat, cow, chicken, turtle, and frog. The gel patterns indicate that the NHC proteins have changed much more during evolution than have the histones; the total pattern of NHC proteins has not been conserved. However, there does appear to be a conservation of a subset of bands for each tissue investigated. Further chemical analysis will be required to establish the significance of the results."}
{"id": "PMID:1080520", "title": "Local adoptive transfer of the antitumor cellular immune response in syngeneic and allogeneic mice studied with a rapid radioisotopic footpad assay.", "content": "The immunologic nature of the cellular immune response against tumor cells inoculated in the footpad of mice was studied with a rapid, quantitative, and specific assay. The results indicate: a) The antitumor cellular immune response could be transferred adoptively in syngeneic and allogeneic mice with specific immune thymus (T) lymphocytes isolated on nylon columns; b) T-independent cells of host origin were necessary for the manifestation of the antitumor footpad reaction; and c) there was a close correlation between immune responses detected by the footpad assay and those detected by transplantation techniques. The footpad reaction consisted of several nonspecific and specific components. Nonspecific factors disturbing the specific footpad reaction in syngeneic and allogeneic recipients were discussed.", "contents": "Local adoptive transfer of the antitumor cellular immune response in syngeneic and allogeneic mice studied with a rapid radioisotopic footpad assay. The immunologic nature of the cellular immune response against tumor cells inoculated in the footpad of mice was studied with a rapid, quantitative, and specific assay. The results indicate: a) The antitumor cellular immune response could be transferred adoptively in syngeneic and allogeneic mice with specific immune thymus (T) lymphocytes isolated on nylon columns; b) T-independent cells of host origin were necessary for the manifestation of the antitumor footpad reaction; and c) there was a close correlation between immune responses detected by the footpad assay and those detected by transplantation techniques. The footpad reaction consisted of several nonspecific and specific components. Nonspecific factors disturbing the specific footpad reaction in syngeneic and allogeneic recipients were discussed."}
{"id": "PMID:1080521", "title": "Daily intravenous infusions of Corynebacterium parvum in twenty patients with disseminated cancer: a preliminary report of clinical and biologic findings.", "content": "Twenty terminally ill patients with various disseminated tumors were treated with daily iv infusions of Corynebacterium parvum given alone at doses of 4 mg/day, 5 days/week, for 4-16 weeks. In 8 patients (40%), the lesions partially regressed to less than 50% of their original size. Another patient who did not improve with C. parvum therapy had a complete remission after the first course of chemotherapy. Skin tests, total leukocyte counts, and T- and B-cell counts revealed variable and unpredictable changes. Phytohemagglutinin- and concanavalin A-induced blastogenesis tended to increase. Of 10 patients, 8 had a significant decrease in serum C3 levels after completion of C. parvum therapy, possibly due to an increased C3 consumption by macrophages activated by the immunostimulant. That nonspecific immune stimulation after repeated iv infusions of an immunostimulant can by itself induce regression in disseminated disease does not agree with the current concept that immunotherapy can be effective only against minimal residual disease. The therapeutic procedure proposed here, though frequently associated with moderate short-lasting side effects, is devoid of serious toxicity.", "contents": "Daily intravenous infusions of Corynebacterium parvum in twenty patients with disseminated cancer: a preliminary report of clinical and biologic findings. Twenty terminally ill patients with various disseminated tumors were treated with daily iv infusions of Corynebacterium parvum given alone at doses of 4 mg/day, 5 days/week, for 4-16 weeks. In 8 patients (40%), the lesions partially regressed to less than 50% of their original size. Another patient who did not improve with C. parvum therapy had a complete remission after the first course of chemotherapy. Skin tests, total leukocyte counts, and T- and B-cell counts revealed variable and unpredictable changes. Phytohemagglutinin- and concanavalin A-induced blastogenesis tended to increase. Of 10 patients, 8 had a significant decrease in serum C3 levels after completion of C. parvum therapy, possibly due to an increased C3 consumption by macrophages activated by the immunostimulant. That nonspecific immune stimulation after repeated iv infusions of an immunostimulant can by itself induce regression in disseminated disease does not agree with the current concept that immunotherapy can be effective only against minimal residual disease. The therapeutic procedure proposed here, though frequently associated with moderate short-lasting side effects, is devoid of serious toxicity."}
{"id": "PMID:1080522", "title": "Transplantation of human cancers to nude mice and effects of thymus grafts.", "content": "Human cancers were transplanted to nude mice; a cell line originating in the foregut (distal stomach or first portion of the duodenum) produced exponential nonmetastasizing growth, preventable by prior thymus implantation. Implantation of thymuses into mice with established tumors resulted in increased animal survival and in four instances in complete regression of the neoplasm. Cell lines of pancreatic carcinoma and melanoma and surgical specimens of melanoma and of neoplasms from the pancreas and colon showed variable growth patterns in this system. Repeated attempts to transplant gastric adenocarcinoma were unsuccessful.", "contents": "Transplantation of human cancers to nude mice and effects of thymus grafts. Human cancers were transplanted to nude mice; a cell line originating in the foregut (distal stomach or first portion of the duodenum) produced exponential nonmetastasizing growth, preventable by prior thymus implantation. Implantation of thymuses into mice with established tumors resulted in increased animal survival and in four instances in complete regression of the neoplasm. Cell lines of pancreatic carcinoma and melanoma and surgical specimens of melanoma and of neoplasms from the pancreas and colon showed variable growth patterns in this system. Repeated attempts to transplant gastric adenocarcinoma were unsuccessful."}
{"id": "PMID:1080523", "title": "Synthesis of reovirus-specific polypeptides in cells pretreated with cycloheximide.", "content": "When L cells are infected with reovirus in the presence of cycloheximide neither virus-specific polypeptides nor viral double-stranded RNA are synthesized. There is some synthesis of viral single-stranded RNA, transcribed mainly from segments L1, M3, S3, and S4 of the 10 viral genomic segments, and in previous work this has been termed the early mRNA pattern. In an attempt to determine whether these early transcripts are functional mRNA's, the transcripts were allowed to accumulate for a period of 17.5 h at 31 C in cycloheximide-treated cells. The cycloheximide was removed and the cells were exposed for various periods to radioactive amino acids to label any virus-specific polypeptides that might be synthesized. An immunoprecipitation technique was used to separate the viral polypeptides from cellular extracts and this precipitate was then analyzed on sodium dodecyl sulfate-polyacrylamide gels. Within 30 min of cycloheximide removal, four major polypeptides (lambda2, mu0, sigma2a, and sigma3) and two minor polypeptides (lambda1 and mu2) were found. In infected cells without cycloheximide eight viral polypeptides (lambda1, lambda2, mu0, mu2, sigma1, sigma2, sigma2a, sigma3) were found at 17.5 h after infection and the same pattern was found between 3 to 4 h after removal of cycloheximide which had been present for 17.5 h after infection. The latter result shows that the cycloheximide inhibition is reversible and that the cells readily recovered and synthesized the normal complement of viral polypeptides. In one set of experiments cordycepin was added to infected cells immediately after the removal of cycloheximide at 17.5 h to inhibit the synthesis of new viral transcripts. During the succeeding 4 h in the presence of cordycepin, the pattern of protein synthesis was the same as that obtained during the 30 min after cycloheximide removal. It is concluded that the polypeptides formed right after removal of cycloheximide are the translation products of transcripts accumulated during cycloheximide treatment and, therefore, that these transcripts are functional viral mRNA's.", "contents": "Synthesis of reovirus-specific polypeptides in cells pretreated with cycloheximide. When L cells are infected with reovirus in the presence of cycloheximide neither virus-specific polypeptides nor viral double-stranded RNA are synthesized. There is some synthesis of viral single-stranded RNA, transcribed mainly from segments L1, M3, S3, and S4 of the 10 viral genomic segments, and in previous work this has been termed the early mRNA pattern. In an attempt to determine whether these early transcripts are functional mRNA's, the transcripts were allowed to accumulate for a period of 17.5 h at 31 C in cycloheximide-treated cells. The cycloheximide was removed and the cells were exposed for various periods to radioactive amino acids to label any virus-specific polypeptides that might be synthesized. An immunoprecipitation technique was used to separate the viral polypeptides from cellular extracts and this precipitate was then analyzed on sodium dodecyl sulfate-polyacrylamide gels. Within 30 min of cycloheximide removal, four major polypeptides (lambda2, mu0, sigma2a, and sigma3) and two minor polypeptides (lambda1 and mu2) were found. In infected cells without cycloheximide eight viral polypeptides (lambda1, lambda2, mu0, mu2, sigma1, sigma2, sigma2a, sigma3) were found at 17.5 h after infection and the same pattern was found between 3 to 4 h after removal of cycloheximide which had been present for 17.5 h after infection. The latter result shows that the cycloheximide inhibition is reversible and that the cells readily recovered and synthesized the normal complement of viral polypeptides. In one set of experiments cordycepin was added to infected cells immediately after the removal of cycloheximide at 17.5 h to inhibit the synthesis of new viral transcripts. During the succeeding 4 h in the presence of cordycepin, the pattern of protein synthesis was the same as that obtained during the 30 min after cycloheximide removal. It is concluded that the polypeptides formed right after removal of cycloheximide are the translation products of transcripts accumulated during cycloheximide treatment and, therefore, that these transcripts are functional viral mRNA's."}
{"id": "PMID:1080524", "title": "Lymphocytotoxins in vasectomized men.", "content": "Serum samples before and after vasectomy were examined for cold and allogeneic lymphocytotoxic antibodies. The techniques used did not demonstrate a significant increase in the incidence of these antibodies following vasectomy.", "contents": "Lymphocytotoxins in vasectomized men. Serum samples before and after vasectomy were examined for cold and allogeneic lymphocytotoxic antibodies. The techniques used did not demonstrate a significant increase in the incidence of these antibodies following vasectomy."}
{"id": "PMID:1080525", "title": "Jamestown Medical Clinic System.", "content": "Comprehensive health care can be provided in rural and poor areas despite a decreasing number of physicians in those areas. A single physician increased his productivity by 300% to 400% by combining already available resources: specially trained and supervised nurse-practitioners, problem-oriented charting, and multiphasic screening. With emphasis on the nurse-practitioner as the main \"person of contact\" for the patient, the physician is able to shift from 50% to 75% of his usual tasks to these specially trained personnel. Patients have come to regard the physician as a \"consultant\" to the nurse-practitioner.", "contents": "Jamestown Medical Clinic System. Comprehensive health care can be provided in rural and poor areas despite a decreasing number of physicians in those areas. A single physician increased his productivity by 300% to 400% by combining already available resources: specially trained and supervised nurse-practitioners, problem-oriented charting, and multiphasic screening. With emphasis on the nurse-practitioner as the main \"person of contact\" for the patient, the physician is able to shift from 50% to 75% of his usual tasks to these specially trained personnel. Patients have come to regard the physician as a \"consultant\" to the nurse-practitioner."}
{"id": "PMID:1080531", "title": "Selective deficiency of thymus-derived lymphocytes in experimental pyelonephritis.", "content": "Pyelonephritis was induced in experimental animals derived of thymus-derived (T) lymphocytes by adult thymectomy and serial sublethal irradiation. In this model T lymphocytes were reduced to less than 1% of normal adjudged by the in vitro Phytohemagglutinin (PHA) responsiveness of lymphoid cells from deprived animals compared with control animals. Pathologic, bacteriologic and immunologic aspects of renal infection were studied in the T cell-deprived animals during the acute, resolving and chronic stages of pyelonephritis. The experiments have shown that the ablation of lymphocytes did not appreciably alter the course of the disease.", "contents": "Selective deficiency of thymus-derived lymphocytes in experimental pyelonephritis. Pyelonephritis was induced in experimental animals derived of thymus-derived (T) lymphocytes by adult thymectomy and serial sublethal irradiation. In this model T lymphocytes were reduced to less than 1% of normal adjudged by the in vitro Phytohemagglutinin (PHA) responsiveness of lymphoid cells from deprived animals compared with control animals. Pathologic, bacteriologic and immunologic aspects of renal infection were studied in the T cell-deprived animals during the acute, resolving and chronic stages of pyelonephritis. The experiments have shown that the ablation of lymphocytes did not appreciably alter the course of the disease."}
{"id": "PMID:1080534", "title": "Bovine venereal vibriosis. Ultrastructure of endometrial inflammatory lesions.", "content": "The inflammatory lesions in bovine venereal vibriosis have been examined by light and transmission electron microscopy in order to provide a better understanding of host defense of the uterus against bacterial invasion. Neutrophils and eosinophils were found mainly at the endometrial surface and in glandular lumina. Mononuclear cells within the endometrial tissue were identified ultrastructurally. Lymphocytes were most abundant, plasma cells were next in frequency, and mononuclear phagocytes were least often observed. An attempt was made to classify lymphocytes on the basis of presence or absence of a cytoplasmic network of filaments and other ultrastructural characteristics.", "contents": "Bovine venereal vibriosis. Ultrastructure of endometrial inflammatory lesions. The inflammatory lesions in bovine venereal vibriosis have been examined by light and transmission electron microscopy in order to provide a better understanding of host defense of the uterus against bacterial invasion. Neutrophils and eosinophils were found mainly at the endometrial surface and in glandular lumina. Mononuclear cells within the endometrial tissue were identified ultrastructurally. Lymphocytes were most abundant, plasma cells were next in frequency, and mononuclear phagocytes were least often observed. An attempt was made to classify lymphocytes on the basis of presence or absence of a cytoplasmic network of filaments and other ultrastructural characteristics."}
{"id": "PMID:1080550", "title": "Effects of halothane and nitrous oxide anaesthesia on 5-HT turn-over in the rat brain.", "content": "The effects of anaesthesia with halothane and nitrous oxide on the turn-over of 5-HT in the rat brain were analysed. The rate of 5-HT synthesis was estimated on the basis of 5-HTP accumulation following 5-HTP decarboxylase inhibition. Inhalation anaesthetics reduced the rate of 5-HT synthesis in the whole brain, this effect being particularly marked in the forebrain. In addition, the reduction of the rate of 5-HT synthesis was still detected after a large tryptophan loading indicating that halothane and nitrous oxide anaesthesia reduced the activity of tryptophan hydroxylase in vivo. The utilization of 5-HT was also delayed during anaesthesia since the rate of 5-HT disappearance following the inhibition of its synthesis at the decarboxylation step was decreased in the whole brain. In spite of this reduced turnover of 5-HT in the brain of rats exposed to inhalation anaesthetics, the 5-HIAA levels were permanently elevated. This was the result of a partial inhibition of the active elimination of the acidic metabolite from the brain during this kind of anaesthesia.", "contents": "Effects of halothane and nitrous oxide anaesthesia on 5-HT turn-over in the rat brain. The effects of anaesthesia with halothane and nitrous oxide on the turn-over of 5-HT in the rat brain were analysed. The rate of 5-HT synthesis was estimated on the basis of 5-HTP accumulation following 5-HTP decarboxylase inhibition. Inhalation anaesthetics reduced the rate of 5-HT synthesis in the whole brain, this effect being particularly marked in the forebrain. In addition, the reduction of the rate of 5-HT synthesis was still detected after a large tryptophan loading indicating that halothane and nitrous oxide anaesthesia reduced the activity of tryptophan hydroxylase in vivo. The utilization of 5-HT was also delayed during anaesthesia since the rate of 5-HT disappearance following the inhibition of its synthesis at the decarboxylation step was decreased in the whole brain. In spite of this reduced turnover of 5-HT in the brain of rats exposed to inhalation anaesthetics, the 5-HIAA levels were permanently elevated. This was the result of a partial inhibition of the active elimination of the acidic metabolite from the brain during this kind of anaesthesia."}
{"id": "PMID:1080551", "title": "Quantal release parameters during fade of endplate potentials.", "content": "An analog of hemicholinium-3 caused the fade of endplate potentials during repetitive stimulation at low rates. The quantal parameters of release were estimated during steady-state conditions before and in the presence of the drug. The fade of EPP's was associated with a decrease in quantal content (m). The decrease in m was, in turn, associated with a decrease in the binomial parameter n and an increase in the binomial parameter p. The changes in n and p are discussed in relation to available stores of transmitter and the probability of transmitter release.", "contents": "Quantal release parameters during fade of endplate potentials. An analog of hemicholinium-3 caused the fade of endplate potentials during repetitive stimulation at low rates. The quantal parameters of release were estimated during steady-state conditions before and in the presence of the drug. The fade of EPP's was associated with a decrease in quantal content (m). The decrease in m was, in turn, associated with a decrease in the binomial parameter n and an increase in the binomial parameter p. The changes in n and p are discussed in relation to available stores of transmitter and the probability of transmitter release."}
{"id": "PMID:1080552", "title": "Is there any transtubular reabsorption of filtered proteins in rat kidney?", "content": "The bovine protease inhibitor aprotinin (Trasylol) has a high affinity to the kidney and is preferentially pinocytized in the proximal tubule. After i.v. injection of 1mug 124 I aprotinin the blood content decreases to 2.8% of the primary injected amount within 3 hrs, while simultaneously each kidney contains 29%. This substance was used to test whether or not a peptide which is pinocytized, is released in the intact form into the peritubular blood. By a cross circulation technique with two unilaterally nephrectomized rats we were unable to detect any transport of pinocytized, intact peptide through the proximal tubule cell over the observed cross circulation period of 1-8 hrs even when using 5000 times the above dosage. Since the total amount of aprotinin in the kidney is immunologically reactive (ca. 97%), and 65% of the radioactivity in the blood is not reactive after 6 hrs, we believe that the last step in the absorption process consists in digestion inside the lysosomes and instantaneous release of the split products into the blood.", "contents": "Is there any transtubular reabsorption of filtered proteins in rat kidney? The bovine protease inhibitor aprotinin (Trasylol) has a high affinity to the kidney and is preferentially pinocytized in the proximal tubule. After i.v. injection of 1mug 124 I aprotinin the blood content decreases to 2.8% of the primary injected amount within 3 hrs, while simultaneously each kidney contains 29%. This substance was used to test whether or not a peptide which is pinocytized, is released in the intact form into the peritubular blood. By a cross circulation technique with two unilaterally nephrectomized rats we were unable to detect any transport of pinocytized, intact peptide through the proximal tubule cell over the observed cross circulation period of 1-8 hrs even when using 5000 times the above dosage. Since the total amount of aprotinin in the kidney is immunologically reactive (ca. 97%), and 65% of the radioactivity in the blood is not reactive after 6 hrs, we believe that the last step in the absorption process consists in digestion inside the lysosomes and instantaneous release of the split products into the blood."}
{"id": "PMID:1080553", "title": "Aspects of the biochemical pharmacology of cytembena.", "content": "Cytembena, at low concentrations, caused an inhibition of the in vitro growth of L1210 mouse leukaemia cells which could not be reversed by reduced folates, purines, amino acids or deoxyribonucleosides. Invitro experiments with a number of enzymes of folate metabolism produced no evidence that this drug acts as an anti-folate in mammalian tumor colls. However, Cytembena, in therapeutic doses, caused a rapid and extensive inhibition of DNA biosynthesis. There was no inhibition of RNA biosynthesis, but at high doses some inhibition of protein biosynthesis was observed. Deoxyribonucleoside triphosphates accumulated in the presence of Cytembena, suggesting that the inhibition of DNA biosynthesis was at the polymerization stage. However, in vitro experiments failed to demonstrate any direct interaction of Cytmebena with either DNA or DNA polymerase.", "contents": "Aspects of the biochemical pharmacology of cytembena. Cytembena, at low concentrations, caused an inhibition of the in vitro growth of L1210 mouse leukaemia cells which could not be reversed by reduced folates, purines, amino acids or deoxyribonucleosides. Invitro experiments with a number of enzymes of folate metabolism produced no evidence that this drug acts as an anti-folate in mammalian tumor colls. However, Cytembena, in therapeutic doses, caused a rapid and extensive inhibition of DNA biosynthesis. There was no inhibition of RNA biosynthesis, but at high doses some inhibition of protein biosynthesis was observed. Deoxyribonucleoside triphosphates accumulated in the presence of Cytembena, suggesting that the inhibition of DNA biosynthesis was at the polymerization stage. However, in vitro experiments failed to demonstrate any direct interaction of Cytmebena with either DNA or DNA polymerase."}
{"id": "PMID:1080556", "title": "The use of transfer factor in a patient with agammaglobulinemia.", "content": "We have studied a 9-year-old boy with agammaglobulinemia treated for the past 6 years with exogenous gamma-globulin who was noted to have an immunoglobulin (Ig)M level of 35 mg/100 ml and circulating B cells as determined by immunofluorescence. Of the circulating lymphocytes, 41% had alpha-immunoglobulin heavy chains, 3% gamma chains, and 3% mu chains. Synthesis of gamma heavy chain classes showing wide heterogeneity and alpha and mu chains of restricted mobility was demonstrated by radioimmunoelectrophoresis. Because of the patient's poor clinical response to exogenous gamma-globulin administration and the paradoxic presence of circulating B cells, with the capacity to synthesize immunoglobulins in vitro, we elected to begin a course of therapy with transfer factor. After the initial four doses of transfer factor (2 x 10(8) lymphocytes/dose) his serum IgG rose from 50 to 130 mg/100 ml, the same level which he had previously attained during continuous exogenous gamma-globulin therapy. His serum IgG has remained at this level for the past 12 months with trimonthly booster doses of transfer factor. The patient has not required any additional gamma-globulin therapy and he has remained clinically asymptomatic. Our studies in a patient with agammaglobulinemia have shown that transfer factor therapy may affect immunoglobulin synthesis. The concurrent discontinuation of exogenous gamma-globulin administration makes it difficult to attribute the changes to only one or another aspect of therapy. We await further reports of the effects of transfer factor in the therapy of patients with B cell disorders.", "contents": "The use of transfer factor in a patient with agammaglobulinemia. We have studied a 9-year-old boy with agammaglobulinemia treated for the past 6 years with exogenous gamma-globulin who was noted to have an immunoglobulin (Ig)M level of 35 mg/100 ml and circulating B cells as determined by immunofluorescence. Of the circulating lymphocytes, 41% had alpha-immunoglobulin heavy chains, 3% gamma chains, and 3% mu chains. Synthesis of gamma heavy chain classes showing wide heterogeneity and alpha and mu chains of restricted mobility was demonstrated by radioimmunoelectrophoresis. Because of the patient's poor clinical response to exogenous gamma-globulin administration and the paradoxic presence of circulating B cells, with the capacity to synthesize immunoglobulins in vitro, we elected to begin a course of therapy with transfer factor. After the initial four doses of transfer factor (2 x 10(8) lymphocytes/dose) his serum IgG rose from 50 to 130 mg/100 ml, the same level which he had previously attained during continuous exogenous gamma-globulin therapy. His serum IgG has remained at this level for the past 12 months with trimonthly booster doses of transfer factor. The patient has not required any additional gamma-globulin therapy and he has remained clinically asymptomatic. Our studies in a patient with agammaglobulinemia have shown that transfer factor therapy may affect immunoglobulin synthesis. The concurrent discontinuation of exogenous gamma-globulin administration makes it difficult to attribute the changes to only one or another aspect of therapy. We await further reports of the effects of transfer factor in the therapy of patients with B cell disorders."}
{"id": "PMID:1080557", "title": "The sodium pertechnetate Tc 99m scan: an aid in the evaluation of gastrointestinal bleeding.", "content": "Twenty pediatric patients presenting primarily with unexplained gastrointestinal bleeding were evaluated with sodium pertechnetate Tc 99m imaging. Three patients had normal barium enemas and scans consistent with Meckel's diverticulum. These three patients and three additional patients with normal scans underwent surgical exploration. Meckel's diverticula containing gastric mucosa were found in all three patients with positive scans. No diverticula were found in the three patients with normal scans. Four other patients had scans that were considered abnormal but not felt to represent Meckel's diverticula. In one of these patients a radiographic gastrointestinent, a nonspecific terminal ilial ulcer without gastric mucosa was found at surgery. The two other patients had normal radiographic gastrointestinal studies and no further evaluation was carried out. The etiology of gastrointestinal bleeding in pediatric patients is frequently unexplained even after thorough evaluation including celiotomy. The sodium pertechnetate Tc 99m scan is a safe, simple, non-invasive procedure that can demonstrate Meckel's diverticula with greater certainty than the barium enema and can suggest suspicious areas that can then be evaluated by more definitive procedures.", "contents": "The sodium pertechnetate Tc 99m scan: an aid in the evaluation of gastrointestinal bleeding. Twenty pediatric patients presenting primarily with unexplained gastrointestinal bleeding were evaluated with sodium pertechnetate Tc 99m imaging. Three patients had normal barium enemas and scans consistent with Meckel's diverticulum. These three patients and three additional patients with normal scans underwent surgical exploration. Meckel's diverticula containing gastric mucosa were found in all three patients with positive scans. No diverticula were found in the three patients with normal scans. Four other patients had scans that were considered abnormal but not felt to represent Meckel's diverticula. In one of these patients a radiographic gastrointestinent, a nonspecific terminal ilial ulcer without gastric mucosa was found at surgery. The two other patients had normal radiographic gastrointestinal studies and no further evaluation was carried out. The etiology of gastrointestinal bleeding in pediatric patients is frequently unexplained even after thorough evaluation including celiotomy. The sodium pertechnetate Tc 99m scan is a safe, simple, non-invasive procedure that can demonstrate Meckel's diverticula with greater certainty than the barium enema and can suggest suspicious areas that can then be evaluated by more definitive procedures."}
{"id": "PMID:1080558", "title": "The relationship between head circumference and the development of communicating hydrocephalus in infants following intraventricular hemmorrhage.", "content": "Rapidly enlarging head circumference is a standard clinical sign of progressive hydrocephalus in an infant. Six neonates 29 to 36 weeks gestational age, with intraventricular hemorrhage, confirmed by ventricular tap, had head circumferences measured at intervals from birth. The sudden appearance of rapidly expanding head size, not associated with changing clinical status, occurred 9 to 20 days after the estimated time of hemorrhage in all of the infants. Air ventriculography within three days of abnormal acceleration of head circumference growth demonstrated moderately to severely dilated ventricles. The ventricles were probably enlarging slowing from the time of hemorrhage because there was no associated clinical deterioration in the infants coincident with the rapid increase in head circumference. After intraventricular hemorrhage, enlarging head circumference appears to be an insensitive sign of hydrocephalus in premature infants.", "contents": "The relationship between head circumference and the development of communicating hydrocephalus in infants following intraventricular hemmorrhage. Rapidly enlarging head circumference is a standard clinical sign of progressive hydrocephalus in an infant. Six neonates 29 to 36 weeks gestational age, with intraventricular hemorrhage, confirmed by ventricular tap, had head circumferences measured at intervals from birth. The sudden appearance of rapidly expanding head size, not associated with changing clinical status, occurred 9 to 20 days after the estimated time of hemorrhage in all of the infants. Air ventriculography within three days of abnormal acceleration of head circumference growth demonstrated moderately to severely dilated ventricles. The ventricles were probably enlarging slowing from the time of hemorrhage because there was no associated clinical deterioration in the infants coincident with the rapid increase in head circumference. After intraventricular hemorrhage, enlarging head circumference appears to be an insensitive sign of hydrocephalus in premature infants."}
{"id": "PMID:1080560", "title": "Effects of waterbed flotation on premature infants: A pilot study.", "content": "Two types of waterbeds were developed to impart compensatory vestibular-proprioceptive stimulation to premature infants. Twenty-one infants ranging in gestational age from 27 to 34 weeks and birthweights from 1,050 to 1,920 gm were included in this pilot study. Assignment to experimental and control groups was made by random design. The experimental group consisted of ten infants who were placed on a gently oscillating waterbed before the sixth postnatal day, where they remained for seven days. Their clinical progress was compared with that of a control group of 11 similar babies. Waterbed flotation was found to be a safe procedure; there was no significant effects on the infants' vital signs, weight, or frequency of emesis. Highly significant differences were found in the incidence of apnea between the two groups, with infants on the oscillating waterbed having significantly fewer apneic spells. Infants placed on the waterbed during the first four postnatal days benefited more than those placed later. A non-oscillating waterbed was found clinically useful for very small prematures with severe skin problems, for infants recovering from abdominal surgery, and for infants receiving parenteral nutrition.", "contents": "Effects of waterbed flotation on premature infants: A pilot study. Two types of waterbeds were developed to impart compensatory vestibular-proprioceptive stimulation to premature infants. Twenty-one infants ranging in gestational age from 27 to 34 weeks and birthweights from 1,050 to 1,920 gm were included in this pilot study. Assignment to experimental and control groups was made by random design. The experimental group consisted of ten infants who were placed on a gently oscillating waterbed before the sixth postnatal day, where they remained for seven days. Their clinical progress was compared with that of a control group of 11 similar babies. Waterbed flotation was found to be a safe procedure; there was no significant effects on the infants' vital signs, weight, or frequency of emesis. Highly significant differences were found in the incidence of apnea between the two groups, with infants on the oscillating waterbed having significantly fewer apneic spells. Infants placed on the waterbed during the first four postnatal days benefited more than those placed later. A non-oscillating waterbed was found clinically useful for very small prematures with severe skin problems, for infants recovering from abdominal surgery, and for infants receiving parenteral nutrition."}
{"id": "PMID:1080562", "title": "Influence of receptor-receptor fibres on the spontaneous afferent activity from semicircular canals in the frog (Rana esculenta).", "content": "The influence of the efferent vestibular system having been eliminated, the spontaneous activity of afferent fibres of the ampullary nerves of the horizontal and vertical anterior semicircular canals was recorded in the frog. By stimulating the ampullary nerve of one of these two canals while recording on the other, as well as by sectioning branches of the vestibular nerve and then using statistical methods, it is shown that receptor-receptor fibres have an inhibitory action on afferent activity from the horizontal and vertical anterior semicircular canals.", "contents": "Influence of receptor-receptor fibres on the spontaneous afferent activity from semicircular canals in the frog (Rana esculenta). The influence of the efferent vestibular system having been eliminated, the spontaneous activity of afferent fibres of the ampullary nerves of the horizontal and vertical anterior semicircular canals was recorded in the frog. By stimulating the ampullary nerve of one of these two canals while recording on the other, as well as by sectioning branches of the vestibular nerve and then using statistical methods, it is shown that receptor-receptor fibres have an inhibitory action on afferent activity from the horizontal and vertical anterior semicircular canals."}
{"id": "PMID:1080568", "title": "Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.", "content": "The availability of anti-phosphocholine antibody of the TEPC 15 idiotype from the clonal progeny of a single precursor cell, stimulated in vitro, permitted the demonstration of monoclonal antibodies with as many as three immunoglobulin classes with identical variable regions. This demonstration was dependent on sensitive radioimmunoassays which showed a one to one relationship between the total anti-phosphocholine antibody produced by a clone and the sum of anti-phosphocholine antibody of the different classes as well as the amount of antibody of the TEPC 15 idiotype. The class distribution was confirmed by isoelectric focusing identification of IgM, Igta, and IgGl antibodies of the TEPC 15 idiotype produced by single clones which showed characteristic pI values for each immunoglobulin class. Thus, within the generative phase of a single antibody-producing cell clone, various heavy chain constant regions can be linked to the same variable region, and single precursor cells have the capacity to generate progeny expressing at least three distinct immunoglobulin classes.", "contents": "Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell. The availability of anti-phosphocholine antibody of the TEPC 15 idiotype from the clonal progeny of a single precursor cell, stimulated in vitro, permitted the demonstration of monoclonal antibodies with as many as three immunoglobulin classes with identical variable regions. This demonstration was dependent on sensitive radioimmunoassays which showed a one to one relationship between the total anti-phosphocholine antibody produced by a clone and the sum of anti-phosphocholine antibody of the different classes as well as the amount of antibody of the TEPC 15 idiotype. The class distribution was confirmed by isoelectric focusing identification of IgM, Igta, and IgGl antibodies of the TEPC 15 idiotype produced by single clones which showed characteristic pI values for each immunoglobulin class. Thus, within the generative phase of a single antibody-producing cell clone, various heavy chain constant regions can be linked to the same variable region, and single precursor cells have the capacity to generate progeny expressing at least three distinct immunoglobulin classes."}
{"id": "PMID:1080569", "title": "Aspects of the psychology and epidemiology of rheumatoid disease.", "content": "In a prospective study of rheumatoid disease (RD) clinical, serological, radiological, and biochemical factors were assessed on 102 patients and scores obtained on the Middlesex Hospital Questionnaire (MHQ). Patients with early RD had MHQ scores closely resembling those in the normal population. Using a new clinical prognostic index, patients who develop severe RD tended to have low scores at initial testing on the MHQ. Patients whose serum was positive for rheumatoid factor also tended to have low scores on the MHQ. Together these findings suggest a possible subclassification of RD into a less severe form in which psychosocial factors may be important, and a more severe form in which heredity or some other constitutional factor (or factors) may be important, rheumatoid factor acting as a 'marker'.", "contents": "Aspects of the psychology and epidemiology of rheumatoid disease. In a prospective study of rheumatoid disease (RD) clinical, serological, radiological, and biochemical factors were assessed on 102 patients and scores obtained on the Middlesex Hospital Questionnaire (MHQ). Patients with early RD had MHQ scores closely resembling those in the normal population. Using a new clinical prognostic index, patients who develop severe RD tended to have low scores at initial testing on the MHQ. Patients whose serum was positive for rheumatoid factor also tended to have low scores on the MHQ. Together these findings suggest a possible subclassification of RD into a less severe form in which psychosocial factors may be important, and a more severe form in which heredity or some other constitutional factor (or factors) may be important, rheumatoid factor acting as a 'marker'."}
{"id": "PMID:1080570", "title": "Dietary tryptophan and the selection of ethyl alcohol in different strains of rats.", "content": "Rats of three different strains were tested systematically for their selection of water or ethanol offered in a two-choice situation. In order that preference-aversion curves could be derived, the concentrations of ethanol were increased in strength from 3 to 30% over an 11 or 12 day test sequence. L-tryptophan was added to the rat's diet in 1% or 3% mixtures for a predetermined interval beginning before or during a test for ethanol preference. Given to female hooded rats of the Royal Victoria strain, excess tryptophan elevated the ethanol preference threshold and significantly increased ethanol intake according to the dose administered. However, in male hooded rats of the Long-Evans strain, tryptophan augmented only slightly the selection of ethanol. Male Sprague-Dawley rats, an albino strain, were unaffected by the addition of this essential amino acid to their diet. Although tryptophan may exert its effect on ethanol intake through an alteration in the metabolism of cerebral serotonin, such an effect seems to be dependent upon the strain of rat tested.", "contents": "Dietary tryptophan and the selection of ethyl alcohol in different strains of rats. Rats of three different strains were tested systematically for their selection of water or ethanol offered in a two-choice situation. In order that preference-aversion curves could be derived, the concentrations of ethanol were increased in strength from 3 to 30% over an 11 or 12 day test sequence. L-tryptophan was added to the rat's diet in 1% or 3% mixtures for a predetermined interval beginning before or during a test for ethanol preference. Given to female hooded rats of the Royal Victoria strain, excess tryptophan elevated the ethanol preference threshold and significantly increased ethanol intake according to the dose administered. However, in male hooded rats of the Long-Evans strain, tryptophan augmented only slightly the selection of ethanol. Male Sprague-Dawley rats, an albino strain, were unaffected by the addition of this essential amino acid to their diet. Although tryptophan may exert its effect on ethanol intake through an alteration in the metabolism of cerebral serotonin, such an effect seems to be dependent upon the strain of rat tested."}
{"id": "PMID:1080571", "title": "Screening hallucinogenic drugs: systematic study of three behavioral tests.", "content": "The effects of several hallucinogenic and non-hallucinogenic drugs have been studied on three behavioral tests proposed as useful indexes of hallucinogenic activity: \"head-twitching\" in mice, defecation in an open-field, and suppression of responding on a differential reinforcement of low rates (DRL) schedule of reinforcement. According to the original propositions, after administration of hallucinogenic agents the frequency of head-twitches would increase in mice, the defecation of rats in an open field would decrease without consistent change in ambulation, rearing and grooming, and the responding of rats on a DRL schedule would yield a typical cumulative record pattern. It was found that the head-twitch test was sensitive to mescaline and LSD-25, but not to delta9-THC or to myristicin and elemicin. Besides, the data on interobserver agreement suggested there is a high degree of subjectivity involved in assessing this response. In the open-field test, non-hallucinogenic drugs such as chlorpromazine and apomorphine fell into the hallucinogenic pattern proposed. In addition, the post-injection interval selected seemed to critically affect defecation scores. The DRL \"hallucinogenic\" pattern occurred nonspecifically after administration of hallucinogenic and non-hallucinogenic drugs. It was concluded that the three tests have limited value for screening purposes.", "contents": "Screening hallucinogenic drugs: systematic study of three behavioral tests. The effects of several hallucinogenic and non-hallucinogenic drugs have been studied on three behavioral tests proposed as useful indexes of hallucinogenic activity: \"head-twitching\" in mice, defecation in an open-field, and suppression of responding on a differential reinforcement of low rates (DRL) schedule of reinforcement. According to the original propositions, after administration of hallucinogenic agents the frequency of head-twitches would increase in mice, the defecation of rats in an open field would decrease without consistent change in ambulation, rearing and grooming, and the responding of rats on a DRL schedule would yield a typical cumulative record pattern. It was found that the head-twitch test was sensitive to mescaline and LSD-25, but not to delta9-THC or to myristicin and elemicin. Besides, the data on interobserver agreement suggested there is a high degree of subjectivity involved in assessing this response. In the open-field test, non-hallucinogenic drugs such as chlorpromazine and apomorphine fell into the hallucinogenic pattern proposed. In addition, the post-injection interval selected seemed to critically affect defecation scores. The DRL \"hallucinogenic\" pattern occurred nonspecifically after administration of hallucinogenic and non-hallucinogenic drugs. It was concluded that the three tests have limited value for screening purposes."}
{"id": "PMID:1080572", "title": "Combined radiosensitizer infusion and irradiation of osteogenic sarcomas.", "content": "Three children with osteogenic sarcomas which were either unresectable or whose parents refused permission to amputate were treated with combined intra-arterial 5'bromodeoxyuridine (BUdR) infusion and high-dose-per-fraction megavoltage irradiation to the primary site. Pulsed, 48-hour BUdR infusions were performed prior to each 600-rad radiation therapy fraction, with a total radiation dose to the primary site of 4,200-4,800 rads in five weeks. Local control was obtained in all 3 children. One child is alive two years after treatment, another died with metastatic disease and the third patient who received radiotherapy to the lungs for pulmonary metastases is without evidence of disease one year later.", "contents": "Combined radiosensitizer infusion and irradiation of osteogenic sarcomas. Three children with osteogenic sarcomas which were either unresectable or whose parents refused permission to amputate were treated with combined intra-arterial 5'bromodeoxyuridine (BUdR) infusion and high-dose-per-fraction megavoltage irradiation to the primary site. Pulsed, 48-hour BUdR infusions were performed prior to each 600-rad radiation therapy fraction, with a total radiation dose to the primary site of 4,200-4,800 rads in five weeks. Local control was obtained in all 3 children. One child is alive two years after treatment, another died with metastatic disease and the third patient who received radiotherapy to the lungs for pulmonary metastases is without evidence of disease one year later."}
{"id": "PMID:1080573", "title": "Neuroradiology of basal anterior fossa (transethmoidal) encephaloceles.", "content": "Encephaloceles of the basal anterior fossa, though primarily congenital in origin, may be insidious in presentation and remain undetected until adulthood. Conventional plain films and hypocycloidal tomograms show a basal osseous defect with its margins bevelled toward the nasopharynx. Magnification angiography is the diagnostic study of choice and reveals herniation of the anterior inferior frontal artery through the osseous defect. Pneumoencephalography, the classical technique of investigation, was found to be unrewarding in demonstrating the herniated sac or in determining the presence or absence of brain tissue within the sac; however, it is of value in demonstrating a ventricular communication with the encephalocele and evaluating ventricular dilatation.", "contents": "Neuroradiology of basal anterior fossa (transethmoidal) encephaloceles. Encephaloceles of the basal anterior fossa, though primarily congenital in origin, may be insidious in presentation and remain undetected until adulthood. Conventional plain films and hypocycloidal tomograms show a basal osseous defect with its margins bevelled toward the nasopharynx. Magnification angiography is the diagnostic study of choice and reveals herniation of the anterior inferior frontal artery through the osseous defect. Pneumoencephalography, the classical technique of investigation, was found to be unrewarding in demonstrating the herniated sac or in determining the presence or absence of brain tissue within the sac; however, it is of value in demonstrating a ventricular communication with the encephalocele and evaluating ventricular dilatation."}
{"id": "PMID:1080574", "title": "Accuracy of brain scanning in pediatric craniocerebral neoplasms.", "content": "A total of 2,563 children were studied by cerebral radionclide angiography, immediate postinjection blood-pool imaging, and delayed 2- to 4-hr. brain scanning. Of 110 new neoplasms of the brain or skull examined, 90% were detected by these means. The scan did not appear to be as accurate in craniopharyngiomas (6/10) and brainstem tumors (5/6). Thirty-nine recurrent tumors not studied previously all produced positive brain scans. Meticulous positioning of the pediatric patient is necessary at all times for accurate results.", "contents": "Accuracy of brain scanning in pediatric craniocerebral neoplasms. A total of 2,563 children were studied by cerebral radionclide angiography, immediate postinjection blood-pool imaging, and delayed 2- to 4-hr. brain scanning. Of 110 new neoplasms of the brain or skull examined, 90% were detected by these means. The scan did not appear to be as accurate in craniopharyngiomas (6/10) and brainstem tumors (5/6). Thirty-nine recurrent tumors not studied previously all produced positive brain scans. Meticulous positioning of the pediatric patient is necessary at all times for accurate results."}
{"id": "PMID:1080580", "title": "The diagnosis and management of accidental haemorrhage with associated coagulopathy.", "content": "An approach to the diagnosis and management of accidental haemorrhage with associated coagulation failure is discussed and outlined. It is stressed that special investigations play a minor role in planning management. The main aim should be to achieve rapid delivery, preferably via the vaginal route. About 50% of these cases are associated with uterine atony and inertia. This is probably due to raised local and circulating levels of fibrin/fibrinogen degradation products (FDP). In such cases uterine activity can be restored by the administration of large intravenous doses of Trasylol (Bayer). Life-endangering complications such as acute respiratory and renal failure should be prevented by prophylactic management and by treatment aimed at rapid delivery. The indications for Caesarean section are outlined. The need for careful assessment prior to discharge of these patients and for re-assessment throughout the puerperium is stressed.", "contents": "The diagnosis and management of accidental haemorrhage with associated coagulopathy. An approach to the diagnosis and management of accidental haemorrhage with associated coagulation failure is discussed and outlined. It is stressed that special investigations play a minor role in planning management. The main aim should be to achieve rapid delivery, preferably via the vaginal route. About 50% of these cases are associated with uterine atony and inertia. This is probably due to raised local and circulating levels of fibrin/fibrinogen degradation products (FDP). In such cases uterine activity can be restored by the administration of large intravenous doses of Trasylol (Bayer). Life-endangering complications such as acute respiratory and renal failure should be prevented by prophylactic management and by treatment aimed at rapid delivery. The indications for Caesarean section are outlined. The need for careful assessment prior to discharge of these patients and for re-assessment throughout the puerperium is stressed."}
{"id": "PMID:1080584", "title": "[Plastic enlargement of the left ventricular outflow tract. A new operative method (author's  transl)].", "content": "A new operative technic for widening the stenotic left ventricular outflow (\"left ventricular tunnel\") is described and a clinical case is reported. A vertical incision along the anterior aspect of the aorta descends with a slight angle across the outflow tract of the right ventricle. After aortic valvectomy the ventricular septum is cut between the left and right coronary commissure through its full thickness and the incision extended inferiorly across the stenotic area. A dacron patch is sewn into the gap of the septum and aortotomy in order to widen the area of previous stenosis and prosthetic replacement of the aortic valve is performed. Finally another dacron patch is used to close the defect in the right ventricular outflow tract.", "contents": "[Plastic enlargement of the left ventricular outflow tract. A new operative method (author's  transl)]. A new operative technic for widening the stenotic left ventricular outflow (\"left ventricular tunnel\") is described and a clinical case is reported. A vertical incision along the anterior aspect of the aorta descends with a slight angle across the outflow tract of the right ventricle. After aortic valvectomy the ventricular septum is cut between the left and right coronary commissure through its full thickness and the incision extended inferiorly across the stenotic area. A dacron patch is sewn into the gap of the septum and aortotomy in order to widen the area of previous stenosis and prosthetic replacement of the aortic valve is performed. Finally another dacron patch is used to close the defect in the right ventricular outflow tract."}
{"id": "PMID:1080585", "title": "[Interruption of the aortic arch with tetralogy of Fallot. Report of a successful one-stage correction of both anomalies, and review of the literature (author's transl)].", "content": "In a ten year old girl, a combination of interruption of the aortic arch (IAA) and tetralogy of Fallot was corrected simultaneously using cardiopulmonary bypass. The interruption was bypassed by implantation of the proximal end of the left subclavian artery into the ascending aorta. The simultaneous correction of both malformations was possible by a left anterolateral transsternal thoracotomy. The success of this operation was confirmed by cardiac cathetherization and angiography. As far as we know this the first report of a successful simultaneous correction of this unusual combination of cardiac malformations.", "contents": "[Interruption of the aortic arch with tetralogy of Fallot. Report of a successful one-stage correction of both anomalies, and review of the literature (author's transl)]. In a ten year old girl, a combination of interruption of the aortic arch (IAA) and tetralogy of Fallot was corrected simultaneously using cardiopulmonary bypass. The interruption was bypassed by implantation of the proximal end of the left subclavian artery into the ascending aorta. The simultaneous correction of both malformations was possible by a left anterolateral transsternal thoracotomy. The success of this operation was confirmed by cardiac cathetherization and angiography. As far as we know this the first report of a successful simultaneous correction of this unusual combination of cardiac malformations."}
{"id": "PMID:1080586", "title": "[Surgical treatment of acute dissection of the ascending aorta (author's transl)].", "content": "Since 1959, 51 patients underwent open heart surgery for correction of acute dissection of the ascending aorta. Upon admission 33 patients were severely hypotensive or in porgressive heart failure. Acute aortic insufficiency was found in 24 patients, and hemiplegia or hemiparesis in 4. In 45 patients the ascending aorta was reconstructed with a woven graft. After excision of the dissected part of the aorta primary anastomosis or patch aortoplasty was performed in 6 patients. The aortic valve remained intact in 26 cases, and resuspension of the commissures restored competence of the aortic valve in another 9 patients. Sixteen patients required aortic valve replacement because of disrupture of the commissures. Dissection extended into the coronary ostia in 5 cases. Reconstruction of the coronary system was accomplished by reimplantation of the ostia, interposition of a vein graft or aorto-coronary bypass. Nine patients died within the early postoperative course from uncontrollable hemorrhage (4), further dissection (3) and myocardial infarction (2). Within the first year after surgery another 5 patients died from acute aortic dissection (2), pseudomonas-infection causing rupture of the proximal graft anastomosis (1), and myocardial infarction (2). The contraindications of antihypertensive treatment of actue dissection of the ascending aorta are discussed. We recommend prompt surgical intervention in acute dissecting aneurysm of the ascending aorta.", "contents": "[Surgical treatment of acute dissection of the ascending aorta (author's transl)]. Since 1959, 51 patients underwent open heart surgery for correction of acute dissection of the ascending aorta. Upon admission 33 patients were severely hypotensive or in porgressive heart failure. Acute aortic insufficiency was found in 24 patients, and hemiplegia or hemiparesis in 4. In 45 patients the ascending aorta was reconstructed with a woven graft. After excision of the dissected part of the aorta primary anastomosis or patch aortoplasty was performed in 6 patients. The aortic valve remained intact in 26 cases, and resuspension of the commissures restored competence of the aortic valve in another 9 patients. Sixteen patients required aortic valve replacement because of disrupture of the commissures. Dissection extended into the coronary ostia in 5 cases. Reconstruction of the coronary system was accomplished by reimplantation of the ostia, interposition of a vein graft or aorto-coronary bypass. Nine patients died within the early postoperative course from uncontrollable hemorrhage (4), further dissection (3) and myocardial infarction (2). Within the first year after surgery another 5 patients died from acute aortic dissection (2), pseudomonas-infection causing rupture of the proximal graft anastomosis (1), and myocardial infarction (2). The contraindications of antihypertensive treatment of actue dissection of the ascending aorta are discussed. We recommend prompt surgical intervention in acute dissecting aneurysm of the ascending aorta."}
{"id": "PMID:1080588", "title": "[Surgery of emphysematous bullae and pulmonary cysts (author's transl)].", "content": "Following a survey of operations performed at the Ruhrland clinic, particular reference is made to the surgical treatment of emphysematous bullae and pulmonary cysts. In these cases, the frequent occurrence of a pneumothorax must here be mentioned. These large bullae are however frequently and incorrectly diagnosed as a pneumothorax. The treatment of these emphysematous bullae, which often occur on both sides of the thorax, is by means of a bilateral axillary thoracotomy. This operation can be carried out in one session, provided a distinct improvement of the pulmonal functions is expected.", "contents": "[Surgery of emphysematous bullae and pulmonary cysts (author's transl)]. Following a survey of operations performed at the Ruhrland clinic, particular reference is made to the surgical treatment of emphysematous bullae and pulmonary cysts. In these cases, the frequent occurrence of a pneumothorax must here be mentioned. These large bullae are however frequently and incorrectly diagnosed as a pneumothorax. The treatment of these emphysematous bullae, which often occur on both sides of the thorax, is by means of a bilateral axillary thoracotomy. This operation can be carried out in one session, provided a distinct improvement of the pulmonal functions is expected."}
{"id": "PMID:1080589", "title": "[Surgical therapy of bronchiectasy and its results (author's transl)].", "content": "69 patients suffering from bronchiectasies underwent surgery at the II. Surgical Clinic of the University of Vienna from 1958 to 1972. Analysis of results revealed three groups: 1. 67% of the patients cured completely, 2. 23% significantly improved, 3. 10% no improvement. The evaluation of spirometric measurements of not improved patients showed extensive preoperative obstructive ventilation disturbance.", "contents": "[Surgical therapy of bronchiectasy and its results (author's transl)]. 69 patients suffering from bronchiectasies underwent surgery at the II. Surgical Clinic of the University of Vienna from 1958 to 1972. Analysis of results revealed three groups: 1. 67% of the patients cured completely, 2. 23% significantly improved, 3. 10% no improvement. The evaluation of spirometric measurements of not improved patients showed extensive preoperative obstructive ventilation disturbance."}
{"id": "PMID:1080590", "title": "[Tracheal-stenosis in neonates, infants and small children (author's transl)].", "content": "By modern technics of investigation and treatment cases of stenosis of the trachea even in neonates and infants are found. These are to be treated with all modern possibilities including resection for tumors. New types of tracheal-stenosis are detected.", "contents": "[Tracheal-stenosis in neonates, infants and small children (author's transl)]. By modern technics of investigation and treatment cases of stenosis of the trachea even in neonates and infants are found. These are to be treated with all modern possibilities including resection for tumors. New types of tracheal-stenosis are detected."}
{"id": "PMID:1080591", "title": "[Diagnosis and therapy of pulmonary sequestration (author's transl)].", "content": "Pulmonary sequestration is a rare malformation which is characterised by lacking connection to the bronchial system and by atypical arterial blood supply. Unidentifiable tumorous, cystic or infiltrative lung diseases, especially of the lower lobes, should always lead to aortography to exclude pulmonary sequestration.", "contents": "[Diagnosis and therapy of pulmonary sequestration (author's transl)]. Pulmonary sequestration is a rare malformation which is characterised by lacking connection to the bronchial system and by atypical arterial blood supply. Unidentifiable tumorous, cystic or infiltrative lung diseases, especially of the lower lobes, should always lead to aortography to exclude pulmonary sequestration."}
{"id": "PMID:1080592", "title": "[Operative treatment of intralobar pulmonary sequestration (author's transl)].", "content": "Among 2700 thoracotomies at the Ruhrlandklinik Essen we have operated eight cases of intralobar pulmonary sequestration. The malformation was found to be situated four times in the left lower lobe, three times in the right lower lobe, and one time in the middle lobe. The only way of curing the suffering consists of surgical interference. At six of eight cases we had to performe a lobectomy, two patients with obviously demarcated alterations have been treated by an exstirpation.", "contents": "[Operative treatment of intralobar pulmonary sequestration (author's transl)]. Among 2700 thoracotomies at the Ruhrlandklinik Essen we have operated eight cases of intralobar pulmonary sequestration. The malformation was found to be situated four times in the left lower lobe, three times in the right lower lobe, and one time in the middle lobe. The only way of curing the suffering consists of surgical interference. At six of eight cases we had to performe a lobectomy, two patients with obviously demarcated alterations have been treated by an exstirpation."}
{"id": "PMID:1080593", "title": "[Surgical treatment of hyperplastic inborn failures of the lung (author's transl)].", "content": "A very rare hyperplastic inborn failure regarding construction of the lung is the accessory lung. It will be reported of an accessory lung in the left mediastinum (without connection to the bronchial system) and of a trachea-accessory lung (with connection to the bronchial system). Both cases had caused symptoms which required a surgical elimination of the accessory tissue. In the first case the left lung had been displaced due to the production of secretion - formation cyst. In the second case the unsufficient secretion flow off had caused repeated pneumonia and formation of bronchiectasis.", "contents": "[Surgical treatment of hyperplastic inborn failures of the lung (author's transl)]. A very rare hyperplastic inborn failure regarding construction of the lung is the accessory lung. It will be reported of an accessory lung in the left mediastinum (without connection to the bronchial system) and of a trachea-accessory lung (with connection to the bronchial system). Both cases had caused symptoms which required a surgical elimination of the accessory tissue. In the first case the left lung had been displaced due to the production of secretion - formation cyst. In the second case the unsufficient secretion flow off had caused repeated pneumonia and formation of bronchiectasis."}
{"id": "PMID:1080594", "title": "[Pulmonary varices (author's transl)].", "content": "In world literature up to now 41 cases of pulmonary varices have been described. Two own cases showed particulars in so far as one patient had an internal fistula between a varix in the base of the upper lobe of the lung and another varix in the apex of the lower lobe. Besides a varix the second patient had an AV-fistula in the right middle lobe. Already pre-operatively either case was diagnosed as vascular anomaly by angiography. By injecting the contrast medium into the vessels of the resection specimen a clear representation of the varices could be obtained.", "contents": "[Pulmonary varices (author's transl)]. In world literature up to now 41 cases of pulmonary varices have been described. Two own cases showed particulars in so far as one patient had an internal fistula between a varix in the base of the upper lobe of the lung and another varix in the apex of the lower lobe. Besides a varix the second patient had an AV-fistula in the right middle lobe. Already pre-operatively either case was diagnosed as vascular anomaly by angiography. By injecting the contrast medium into the vessels of the resection specimen a clear representation of the varices could be obtained."}
{"id": "PMID:1080595", "title": "[Diangosis and treatment of pulmonary arteriovenous fistula in infancy (author's transl)].", "content": "Pulmonary arteriovenous fistulas are seldom diagnosed in infancy because of few signs and symptoms in early life. The clinical picture is due to the right-to-left shunt and includes dyspnea, cyanosis, polycythemia, clubbing, and murmur over the fistula. The diagnosis can be suspected by demonstration of a typical density on chest X-ray. It can be established by angiocardiography. Because of the life-threatening complications resectional treatment is recommended already in infancy. A case is reported, diagnosed on the second day of life by angiocardiography and treated 3 months later by lobectomy.", "contents": "[Diangosis and treatment of pulmonary arteriovenous fistula in infancy (author's transl)]. Pulmonary arteriovenous fistulas are seldom diagnosed in infancy because of few signs and symptoms in early life. The clinical picture is due to the right-to-left shunt and includes dyspnea, cyanosis, polycythemia, clubbing, and murmur over the fistula. The diagnosis can be suspected by demonstration of a typical density on chest X-ray. It can be established by angiocardiography. Because of the life-threatening complications resectional treatment is recommended already in infancy. A case is reported, diagnosed on the second day of life by angiocardiography and treated 3 months later by lobectomy."}
{"id": "PMID:1080596", "title": "[Solitary pulmonary arteriovenous fistula in infancy and childhood (author's transl)].", "content": "Pulmonary arteriovenous fistula is not a rare congenital malformation, but obviously most often it is a very late diagnosis. Moreover the disease is associated with a high risk of serious complications, namely cerebrovascular accidents. Two patients, an one month old infant and an 8 years old boy with typical symptoms and signs are presented. Both of them were operated upon successfully. It is concluded, that the diagnosis of solitary pulmonary arteriovenous fistula should lead to immediate operation, which is technically easy and of low risk only, even in infancy. Conservative surgery is recommended without sacrifice of healthy lung tissue, i.e. local excision or segmental resection in subpleural lesions and lobectomy for deep-seated fistulas.", "contents": "[Solitary pulmonary arteriovenous fistula in infancy and childhood (author's transl)]. Pulmonary arteriovenous fistula is not a rare congenital malformation, but obviously most often it is a very late diagnosis. Moreover the disease is associated with a high risk of serious complications, namely cerebrovascular accidents. Two patients, an one month old infant and an 8 years old boy with typical symptoms and signs are presented. Both of them were operated upon successfully. It is concluded, that the diagnosis of solitary pulmonary arteriovenous fistula should lead to immediate operation, which is technically easy and of low risk only, even in infancy. Conservative surgery is recommended without sacrifice of healthy lung tissue, i.e. local excision or segmental resection in subpleural lesions and lobectomy for deep-seated fistulas."}
{"id": "PMID:1080597", "title": "[The congenital lobar emphysema--a case of emergency for the thoracical surgery during the early infancy (author's transl)].", "content": "The congenital lobar emphysema is a not very often found malformation. It causes during the first respirations a excessive, almost unilateral, segmental or lobar expansion. This ensues quickly a unilateral, intrathoracic high pressure with atelectasis, displacement of the mediastinum to the otherside and following extensive respiratory and circulatory derangements. The fully developed syndrom may lead to death very quickly. If diagnosis is assured, therapy of choice is to resect the involved parts of the lung.", "contents": "[The congenital lobar emphysema--a case of emergency for the thoracical surgery during the early infancy (author's transl)]. The congenital lobar emphysema is a not very often found malformation. It causes during the first respirations a excessive, almost unilateral, segmental or lobar expansion. This ensues quickly a unilateral, intrathoracic high pressure with atelectasis, displacement of the mediastinum to the otherside and following extensive respiratory and circulatory derangements. The fully developed syndrom may lead to death very quickly. If diagnosis is assured, therapy of choice is to resect the involved parts of the lung."}
{"id": "PMID:1080598", "title": "[Lobar emphysema: differential diagnosis and treatment (author's transl)].", "content": "Lobar emphysema with acute severe respiratory distress is predominately seen in early infancy while the few cases after one year of age usually show signs of recurrent respiratory infection. Several conditions causing increased transparency on one side of the chest x-ray must be be differentiated. In case of emergency the simple chest x-ray must be sufficient for diagnosis. In this situation even the performance of a diagnostic thoracotomy can become necessary. The removal of the affected lobe is the treatment of choice. 6 cases of lobar emphysema are reported.", "contents": "[Lobar emphysema: differential diagnosis and treatment (author's transl)]. Lobar emphysema with acute severe respiratory distress is predominately seen in early infancy while the few cases after one year of age usually show signs of recurrent respiratory infection. Several conditions causing increased transparency on one side of the chest x-ray must be be differentiated. In case of emergency the simple chest x-ray must be sufficient for diagnosis. In this situation even the performance of a diagnostic thoracotomy can become necessary. The removal of the affected lobe is the treatment of choice. 6 cases of lobar emphysema are reported."}
{"id": "PMID:1080599", "title": "[Differentiated surgical therapy in congenital lobar emphysema (author's transl)].", "content": "Congenital lobar emphysema (CLE) is a clinical and radiological entity with a multiplicity of etiological factors. Six patients are presented with CLE of the left upper lobe. In three of them CLE was associated with cardiovascular malformation i.e. persisting ductus arteriosus and in one case additionally with double aortic arch. These infants were treated by division of the compressing vascular structure without lobectomy. Only one required secondary resection. Left sided CLE presumably caused by vascular compression should be considered a special group in which surgical correction without lung resection is justified.", "contents": "[Differentiated surgical therapy in congenital lobar emphysema (author's transl)]. Congenital lobar emphysema (CLE) is a clinical and radiological entity with a multiplicity of etiological factors. Six patients are presented with CLE of the left upper lobe. In three of them CLE was associated with cardiovascular malformation i.e. persisting ductus arteriosus and in one case additionally with double aortic arch. These infants were treated by division of the compressing vascular structure without lobectomy. Only one required secondary resection. Left sided CLE presumably caused by vascular compression should be considered a special group in which surgical correction without lung resection is justified."}
{"id": "PMID:1080600", "title": "Congenital lobar emphysema.", "content": "43 patients with congenital lobar emphysema were treated surgically at the Thoracic Unit, Hospital for Sick Children, Great Ormond Street, London, between 1954 and 1974. Nearly half of the infants had additional congenital anomalies. 7 children died after operation.", "contents": "Congenital lobar emphysema. 43 patients with congenital lobar emphysema were treated surgically at the Thoracic Unit, Hospital for Sick Children, Great Ormond Street, London, between 1954 and 1974. Nearly half of the infants had additional congenital anomalies. 7 children died after operation."}
{"id": "PMID:1080601", "title": "[The transsternal and transpericardial approach for surgical treatment of fistulas of the main bronchus after pneumonectomy (author's transl)].", "content": "The management of bronchopleural fistula after lung resection is discussed. The transsternal and transpericardial approach for surgical treatment of fistulas of the main bronchus after pneumonectomy is described. In 4 cases of 2000 lung resections occurred a bronchopleural fistula after right pneumonectomy. In all cases the fistula was closed definitive. In one case developed a fistula between the pleural space and the oesophagus after operation.", "contents": "[The transsternal and transpericardial approach for surgical treatment of fistulas of the main bronchus after pneumonectomy (author's transl)]. The management of bronchopleural fistula after lung resection is discussed. The transsternal and transpericardial approach for surgical treatment of fistulas of the main bronchus after pneumonectomy is described. In 4 cases of 2000 lung resections occurred a bronchopleural fistula after right pneumonectomy. In all cases the fistula was closed definitive. In one case developed a fistula between the pleural space and the oesophagus after operation."}
{"id": "PMID:1080602", "title": "[Transbronchial brush biopsy under TV-fluoroscopy control (author's transl)].", "content": "Transbronchial brush biopsy is indicated when routine methods fail to establish the diagnosis of a pulmonary lesion. More than 50 biopsies have been performed without complications. The diagnostic accuracy of about 50% positive reports will definitively improve with the better selection of cases and with greater experience with this technique.", "contents": "[Transbronchial brush biopsy under TV-fluoroscopy control (author's transl)]. Transbronchial brush biopsy is indicated when routine methods fail to establish the diagnosis of a pulmonary lesion. More than 50 biopsies have been performed without complications. The diagnostic accuracy of about 50% positive reports will definitively improve with the better selection of cases and with greater experience with this technique."}
{"id": "PMID:1080603", "title": "[Histological and histochemical evaluation of the cellular infiltration of s.c. implanted sparks prothesis. Experiments on rats (authors transl)].", "content": "Sparks-prothesis of 3 mm diameter were implanted into inbred rats and withdrawn after one to ten weeks. They were then fixed in Formalin and embedded in Paraffin and Metachrylat. After cutting (2.5 mu), the histological and histochemical parameters were examined. Cellular infiltration was studied after a certain period of time. The basic results of the genesis of that infiltration are given as follows: during the first two weeks following the s.c. implantation, derivates of monocytes (cells which play an imminent and important role during arterial replacement) were found. After the third and fourth week collagenous and elastic fibers could be seen, the connective tissue became stronger. The most favorable time for grafting can be evaluated by these experiments.", "contents": "[Histological and histochemical evaluation of the cellular infiltration of s.c. implanted sparks prothesis. Experiments on rats (authors transl)]. Sparks-prothesis of 3 mm diameter were implanted into inbred rats and withdrawn after one to ten weeks. They were then fixed in Formalin and embedded in Paraffin and Metachrylat. After cutting (2.5 mu), the histological and histochemical parameters were examined. Cellular infiltration was studied after a certain period of time. The basic results of the genesis of that infiltration are given as follows: during the first two weeks following the s.c. implantation, derivates of monocytes (cells which play an imminent and important role during arterial replacement) were found. After the third and fourth week collagenous and elastic fibers could be seen, the connective tissue became stronger. The most favorable time for grafting can be evaluated by these experiments."}
{"id": "PMID:1080604", "title": "[Radiographic in vivo visualization of parts of the ventricular conduction system of the heart (author's transl)].", "content": "The in vivo tolerability of an in vitro in cow, calf and sheep hearts developed method for radiographic visualization of parts of the ventricular conduction system is tested in animal experiments.", "contents": "[Radiographic in vivo visualization of parts of the ventricular conduction system of the heart (author's transl)]. The in vivo tolerability of an in vitro in cow, calf and sheep hearts developed method for radiographic visualization of parts of the ventricular conduction system is tested in animal experiments."}
{"id": "PMID:1080605", "title": "[Instantaneous blood flow velocity profiles before and after aortic valve replacement (author's transl)].", "content": "In 17 patients the instantaneous velocity profile was recorded before and after aortic valve replacement (Starr-Edwards, Bj\u00f6rk-Shiley, Lillehei-Kaster). A single gated pulsed Doppler Ultrasonic flownmeter was used for analysis. Distal of these valves a flat flow profile was recorded showing high velocity gradients near the walls. As a main cause of this, numerous eddies and turbulent flow have been suggested. Downstream of a Starr valve a flat profile appears with a slowing down of forward flow just behind the ball. The disk valves Lillehei-Kaster and Bj\u00f6rk-Shiley demonstrated a higher flow velocity in direction to the larger opening. The \"in vivo\" studies have been supported by \"in vitro\" measurements. The highest flow velocities were above 100 cm/sec and thus, according to Reynolds definition, in the turbulent range. The turbulent flow produces a washing effect, which is emphasized during the cardiac cycle. The importance of these data can be discussed by clinical results only.", "contents": "[Instantaneous blood flow velocity profiles before and after aortic valve replacement (author's transl)]. In 17 patients the instantaneous velocity profile was recorded before and after aortic valve replacement (Starr-Edwards, Bj\u00f6rk-Shiley, Lillehei-Kaster). A single gated pulsed Doppler Ultrasonic flownmeter was used for analysis. Distal of these valves a flat flow profile was recorded showing high velocity gradients near the walls. As a main cause of this, numerous eddies and turbulent flow have been suggested. Downstream of a Starr valve a flat profile appears with a slowing down of forward flow just behind the ball. The disk valves Lillehei-Kaster and Bj\u00f6rk-Shiley demonstrated a higher flow velocity in direction to the larger opening. The \"in vivo\" studies have been supported by \"in vitro\" measurements. The highest flow velocities were above 100 cm/sec and thus, according to Reynolds definition, in the turbulent range. The turbulent flow produces a washing effect, which is emphasized during the cardiac cycle. The importance of these data can be discussed by clinical results only."}
{"id": "PMID:1080606", "title": "[Ultrasound transit-time method for evaluation of regional myocardial function (author's transl)].", "content": "The validity of an ultrasound transit-time method for defining local function was evaluated under experimental conditions in the anaesthetized open chest dog. Miniaturized piezoelectric crystals (1,5-2 mm phi) functioning as emitters and receivers were inserted into different depths of the myocardium to study the contraction patterns of defined muscle segments in different muscle layers and the dynamic changes of left ventricular wall thickness during the heart cycle. Changes in myocardial function were induced by coronary stenosis or occlusion and by the application of drugs. This method was shown to be a sensible and reliable tool for the evaluation of local myocardial function.", "contents": "[Ultrasound transit-time method for evaluation of regional myocardial function (author's transl)]. The validity of an ultrasound transit-time method for defining local function was evaluated under experimental conditions in the anaesthetized open chest dog. Miniaturized piezoelectric crystals (1,5-2 mm phi) functioning as emitters and receivers were inserted into different depths of the myocardium to study the contraction patterns of defined muscle segments in different muscle layers and the dynamic changes of left ventricular wall thickness during the heart cycle. Changes in myocardial function were induced by coronary stenosis or occlusion and by the application of drugs. This method was shown to be a sensible and reliable tool for the evaluation of local myocardial function."}
{"id": "PMID:1080607", "title": "[The effects of left ventricular distention on coronary blood flow and myocardial metabolism during cardiopulmonary bypass (author's transl)].", "content": "These studies show that distention of the fibrillating left ventricle during cardiopulmonary bypass impedes subendocardial flow and causes myocardial ischemia. While myocardial oxygen requirements increase when the heart becomes distended (La Place relationship) the elevated intracavitary pressure acts as a counterforce to subendocardial flow and prevents it from increasing sufficiently to meet these increased metabolic demands. The resultant ischemia is accentuated when the fibrillation is maintained electrically, since the fibrillating stimulus itself impedes subendocardial flow and causes ischemia.", "contents": "[The effects of left ventricular distention on coronary blood flow and myocardial metabolism during cardiopulmonary bypass (author's transl)]. These studies show that distention of the fibrillating left ventricle during cardiopulmonary bypass impedes subendocardial flow and causes myocardial ischemia. While myocardial oxygen requirements increase when the heart becomes distended (La Place relationship) the elevated intracavitary pressure acts as a counterforce to subendocardial flow and prevents it from increasing sufficiently to meet these increased metabolic demands. The resultant ischemia is accentuated when the fibrillation is maintained electrically, since the fibrillating stimulus itself impedes subendocardial flow and causes ischemia."}
{"id": "PMID:1080608", "title": "[Regional myocardial blood flow and left ventricular function in critical coronary stenosis and after coronary artery bypass grafting in the dog (author's transl)].", "content": "In 7 mongrel dogs myocardial ischemia is produced by means of an ameroid constrictor placed on the left circumflex coronary artery (CCA). Myocardial blood flow (MBF) is diminished by 56% in an area of 25% of the anterior wall of the left ventricle. A decrease in dp/dtmax suggests a small depression of left ventricular function. After bypass operation the slight changes in EDP, dp/dtmax and Vmax indicate no significant improvement of total ventricular function. MBF increases from 42 +/- 10 ml/100 g/min to 67 +/- 21 ml/100 g/min (p smaller than 0.02) in the area supplied by the bypass. In this region intramyocardial pressure rises significantly suggesting improvement of regional myocardial function.", "contents": "[Regional myocardial blood flow and left ventricular function in critical coronary stenosis and after coronary artery bypass grafting in the dog (author's transl)]. In 7 mongrel dogs myocardial ischemia is produced by means of an ameroid constrictor placed on the left circumflex coronary artery (CCA). Myocardial blood flow (MBF) is diminished by 56% in an area of 25% of the anterior wall of the left ventricle. A decrease in dp/dtmax suggests a small depression of left ventricular function. After bypass operation the slight changes in EDP, dp/dtmax and Vmax indicate no significant improvement of total ventricular function. MBF increases from 42 +/- 10 ml/100 g/min to 67 +/- 21 ml/100 g/min (p smaller than 0.02) in the area supplied by the bypass. In this region intramyocardial pressure rises significantly suggesting improvement of regional myocardial function."}
{"id": "PMID:1080609", "title": "[Coronary circulation and myocardial oxygen cosumption after cardioplegic arrest (author's transl)].", "content": "Coronary blood flow, myocardial oxygen consumption, and cardiac hemodynamics was measured before and after cardiopulmonary bypass in two group of patients (7 and 4) with minor congenital heart disease where cardiac arrest was induced by means of \"cardioplegin\" or \"external surface cooling\" (Shumway). Only myocardial oxygen consumption was reduced 20% after bypass in the \"cardioplegin\" group; all other measured parameters remained essentially constant.", "contents": "[Coronary circulation and myocardial oxygen cosumption after cardioplegic arrest (author's transl)]. Coronary blood flow, myocardial oxygen consumption, and cardiac hemodynamics was measured before and after cardiopulmonary bypass in two group of patients (7 and 4) with minor congenital heart disease where cardiac arrest was induced by means of \"cardioplegin\" or \"external surface cooling\" (Shumway). Only myocardial oxygen consumption was reduced 20% after bypass in the \"cardioplegin\" group; all other measured parameters remained essentially constant."}
{"id": "PMID:1080610", "title": "[Metabolism and ultrastructure of magnesium aspartate-procaine arrested hearts of rabbit and man (author's transl)].", "content": "In normothermia, mild, and deep hypothermia the metabolism and the electron microscopic structure were investigated in human and rabbit heart muscle after magnesium aspartate-procaine cardioplegia. In comparison to plain ischaemic arrest splitting of adenine nucleotides and glycogen was significantly reduced in all experiments with the induced cardioplegic arrest. For 40 min at 32 degrees C almost no changes in ultrastructure were seen in heart muscle after induced arrest, while severe and/or irreversible damages were seen in the cell structure of the heart muscle due to plain ischaemic arrest.", "contents": "[Metabolism and ultrastructure of magnesium aspartate-procaine arrested hearts of rabbit and man (author's transl)]. In normothermia, mild, and deep hypothermia the metabolism and the electron microscopic structure were investigated in human and rabbit heart muscle after magnesium aspartate-procaine cardioplegia. In comparison to plain ischaemic arrest splitting of adenine nucleotides and glycogen was significantly reduced in all experiments with the induced cardioplegic arrest. For 40 min at 32 degrees C almost no changes in ultrastructure were seen in heart muscle after induced arrest, while severe and/or irreversible damages were seen in the cell structure of the heart muscle due to plain ischaemic arrest."}
{"id": "PMID:1080611", "title": "[The effect of ischemic and cardioplegic heart standstill on the myocard metabolism in normal and less perfused heart (author's transl)].", "content": "The tolerance of ischemia in normal and less perfused myocard during an ischemic and cardioplegic heart standstill was investigated. A decrease of ATP and ADP and an increase of the lactate, pyruvate and AMP was established during an ischemia of 20 minutes. The less perfused hearts gave considerably worse results regarding the energy transformation than normal hearts. In less perfused hearts the energy reserve of myocardial metabolism showed a considerably better behaviour during a cardioplegic heart standstill of 45 minutes than an ischemic one of 20 minutes. During the recuperation period this trend was emphasised.", "contents": "[The effect of ischemic and cardioplegic heart standstill on the myocard metabolism in normal and less perfused heart (author's transl)]. The tolerance of ischemia in normal and less perfused myocard during an ischemic and cardioplegic heart standstill was investigated. A decrease of ATP and ADP and an increase of the lactate, pyruvate and AMP was established during an ischemia of 20 minutes. The less perfused hearts gave considerably worse results regarding the energy transformation than normal hearts. In less perfused hearts the energy reserve of myocardial metabolism showed a considerably better behaviour during a cardioplegic heart standstill of 45 minutes than an ischemic one of 20 minutes. During the recuperation period this trend was emphasised."}
{"id": "PMID:1080612", "title": "Complement-activating properties of some rat alloantibodies.", "content": "The complement-activating property of immune sera, and of separated 19S and 7S antibody fractions, was examined with a number of rat histocompatibility antigen-alloantibody systems. It was confirmed that xenogeneic sera are very efficient sources of complement in the in vitro immune cytolysis of rat lymphocytes and erythrocytes by the alloantibodies of 19S and 7S classes. However, with allogeneic complement only 7S alloantibodies were able to lyse rat lymphocytes, and none of several alloimmune sera, nor any fractions derived from them, lysed allogeneic erythrocytes. The evidence suggests that the popular notion that IgM antibodies, as a class, are more efficient than IgG molecules in complement-mediated cell lysis may need qualification as to the source of complement and the type of target cell used in a given-experimental system.", "contents": "Complement-activating properties of some rat alloantibodies. The complement-activating property of immune sera, and of separated 19S and 7S antibody fractions, was examined with a number of rat histocompatibility antigen-alloantibody systems. It was confirmed that xenogeneic sera are very efficient sources of complement in the in vitro immune cytolysis of rat lymphocytes and erythrocytes by the alloantibodies of 19S and 7S classes. However, with allogeneic complement only 7S alloantibodies were able to lyse rat lymphocytes, and none of several alloimmune sera, nor any fractions derived from them, lysed allogeneic erythrocytes. The evidence suggests that the popular notion that IgM antibodies, as a class, are more efficient than IgG molecules in complement-mediated cell lysis may need qualification as to the source of complement and the type of target cell used in a given-experimental system."}
{"id": "PMID:1080613", "title": "Functional characterization of membrane components of cytotoxic peritoneal exudate T lymphocytes. II. Trypsin sensitivity of the killer cell receptor.", "content": "Studies were carried out to investigate the nature of the lymphocyte membrane receptor involved in the specific lysis of [51Cr]EL4 target cells by BALB/c cytotoxic peritoneal exudate lymphocytes (PEL) in vitro. In confirmation of earlier related studies, the cytolytic reaction is markedly inhibited by trypsin treatment (1 mg of trypsin/10(6) PEL/ml; 37 C for 30 min) of the PEL. Trypsinized PEL recover their cytolytic activity after 6 hr of incubation in trypsin-free medium at 37 C; recovery is suppressed at 4 C. Significantly, trypsinization of PEL inhibits their ability to adsorb to EL4 target monolayers, indicating that lymphocyte-target cell binding is a trypsinsensitive step of the cytolytic interaction. Specificity of binding for EL4 is restored upon recovery from trypsin treatment. In addition, trypsin treatment, under conditions sufficient to inhibit cytolysis, causes a drastic nonspecific deletion of iodinatable membrane species from the surface of PEL. Thus, a direct correlation between altered cytolytic function and membrane structure cannot be made. Interestingly, trypsinized PEL retain the capacity to kill EL4 to which they are agglutinated by concanavalin-A, suggesting that the lytic mechanism, as distinguished from the binding receptor, is still intact. Papain and pronase also have an inhibitory effect on cytolysis, while neuraminidase causes a significant augmentation of lymphocyte-mediated cytolysis.", "contents": "Functional characterization of membrane components of cytotoxic peritoneal exudate T lymphocytes. II. Trypsin sensitivity of the killer cell receptor. Studies were carried out to investigate the nature of the lymphocyte membrane receptor involved in the specific lysis of [51Cr]EL4 target cells by BALB/c cytotoxic peritoneal exudate lymphocytes (PEL) in vitro. In confirmation of earlier related studies, the cytolytic reaction is markedly inhibited by trypsin treatment (1 mg of trypsin/10(6) PEL/ml; 37 C for 30 min) of the PEL. Trypsinized PEL recover their cytolytic activity after 6 hr of incubation in trypsin-free medium at 37 C; recovery is suppressed at 4 C. Significantly, trypsinization of PEL inhibits their ability to adsorb to EL4 target monolayers, indicating that lymphocyte-target cell binding is a trypsinsensitive step of the cytolytic interaction. Specificity of binding for EL4 is restored upon recovery from trypsin treatment. In addition, trypsin treatment, under conditions sufficient to inhibit cytolysis, causes a drastic nonspecific deletion of iodinatable membrane species from the surface of PEL. Thus, a direct correlation between altered cytolytic function and membrane structure cannot be made. Interestingly, trypsinized PEL retain the capacity to kill EL4 to which they are agglutinated by concanavalin-A, suggesting that the lytic mechanism, as distinguished from the binding receptor, is still intact. Papain and pronase also have an inhibitory effect on cytolysis, while neuraminidase causes a significant augmentation of lymphocyte-mediated cytolysis."}
{"id": "PMID:1080616", "title": "Porcine intestinal adenomatosis: a possible relationship with necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy.", "content": "Necrotic enteritis and regional ileitis are recognised causes of sporadic death in pigs. In both conditions, the intestinal mucosa harbours the bacterium, Campylobacter sputorum subsp mucosalis, which was first associated with procine intestinal adenomatosis.", "contents": "Porcine intestinal adenomatosis: a possible relationship with necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy. Necrotic enteritis and regional ileitis are recognised causes of sporadic death in pigs. In both conditions, the intestinal mucosa harbours the bacterium, Campylobacter sputorum subsp mucosalis, which was first associated with procine intestinal adenomatosis."}
{"id": "PMID:1080630", "title": "Serum trypsin inhibitory capacity and pi phenotypes. II. Prevalence of alpha1-antitrypsin deficiency in an allergy population.", "content": "A series of 138 patients seen consecutively in an adult allergy clinic were studied to determine serum alpha1-antitrypsin (alpha1-AT) levels and protease inhibitor (Pi) phenotypes. These were compared with a control group of 700 healthy young adult blood donors. Both total serum trypsin inhibitory capacity (STIC) and alpha1-AT levels as determined by immunoelectrophoresis (IEP) were measured. Phenotypes were determined by antigen-antibody-crossed IEP. Alpha1-AT levels in the allergy group were not significantly different from control values. STIC in the allergy group was 1.34 +/- 0.28 mg. trypsin inhibited per ml. serum and in the controls, 1.32 +/- 0.28 mg. IEP values were 205 +/- vs. 200 +/- 36 mg. per dl. Similarly, no increase in Pi phenotypes known to be related to any disease state was observed. Since Pi phenotypes other than the most common MM are rare in Negro populations, phenotype data were corrected to include only Caucasian subjects. In the allergy group adjusted for racial composition we found 2.86% MZ, 5.72% MS; no SZ, SS or ZZ was detected. A comparable control  group contained 3.68% MZ, 6.23% MS, 0.71% SZ-SS, but no ZZ Pi phenotype.", "contents": "Serum trypsin inhibitory capacity and pi phenotypes. II. Prevalence of alpha1-antitrypsin deficiency in an allergy population. A series of 138 patients seen consecutively in an adult allergy clinic were studied to determine serum alpha1-antitrypsin (alpha1-AT) levels and protease inhibitor (Pi) phenotypes. These were compared with a control group of 700 healthy young adult blood donors. Both total serum trypsin inhibitory capacity (STIC) and alpha1-AT levels as determined by immunoelectrophoresis (IEP) were measured. Phenotypes were determined by antigen-antibody-crossed IEP. Alpha1-AT levels in the allergy group were not significantly different from control values. STIC in the allergy group was 1.34 +/- 0.28 mg. trypsin inhibited per ml. serum and in the controls, 1.32 +/- 0.28 mg. IEP values were 205 +/- vs. 200 +/- 36 mg. per dl. Similarly, no increase in Pi phenotypes known to be related to any disease state was observed. Since Pi phenotypes other than the most common MM are rare in Negro populations, phenotype data were corrected to include only Caucasian subjects. In the allergy group adjusted for racial composition we found 2.86% MZ, 5.72% MS; no SZ, SS or ZZ was detected. A comparable control  group contained 3.68% MZ, 6.23% MS, 0.71% SZ-SS, but no ZZ Pi phenotype."}
{"id": "PMID:1080631", "title": "Parenteral aspirin produces and enhances gastric mucosal lesions and bleeding in rats.", "content": "The effect of parenteral sodium acetylsalicylate (Na ASA) on the development of gastric mucosal lesions and bleeding was studied in control rats and in rats subjected to cold-restraint stress. Although both doses of Na ASA studied, 15 and 60 mg/kg intraperitoneally, alone resulted in lesion formation, only with the larger dose did this reach the level of statistical significance. However both doses of Na ASA alone significantly increased blood loss as measured by a decrease in hematocrit value. The administration of Na ASA 15 MG/kg intraperitoneally to animals subjected to cold-restraint significantly increased blood loss, but not lesion formation, while the larger dose of Na ASA significantly increased both parameters. Thus parenteral aspirin not only can cause gastric mucosal damage and bleeding, but it can enhance lesion formation and blood loss produced by cold-restraint. Two mechanisms may be involved: a smaller dose of Na ASA significantly affecting hemostasis but a larger dose being required for gastric mucosal damage.", "contents": "Parenteral aspirin produces and enhances gastric mucosal lesions and bleeding in rats. The effect of parenteral sodium acetylsalicylate (Na ASA) on the development of gastric mucosal lesions and bleeding was studied in control rats and in rats subjected to cold-restraint stress. Although both doses of Na ASA studied, 15 and 60 mg/kg intraperitoneally, alone resulted in lesion formation, only with the larger dose did this reach the level of statistical significance. However both doses of Na ASA alone significantly increased blood loss as measured by a decrease in hematocrit value. The administration of Na ASA 15 MG/kg intraperitoneally to animals subjected to cold-restraint significantly increased blood loss, but not lesion formation, while the larger dose of Na ASA significantly increased both parameters. Thus parenteral aspirin not only can cause gastric mucosal damage and bleeding, but it can enhance lesion formation and blood loss produced by cold-restraint. Two mechanisms may be involved: a smaller dose of Na ASA significantly affecting hemostasis but a larger dose being required for gastric mucosal damage."}
{"id": "PMID:1080632", "title": "Morphology of chronic lymphocytic leukemia and its relationship to survival.", "content": "The morphology of lymphocytes in blood and bone marrow from patients with chronic lymphocytic leukemia was studied; blood lymphocyte morphology was related to survival. Three primary morphologic groups emerged. Group 1 was characterized by small to medium-sized lymphocytes with narrow rims of cytoplasm and coarsely clumped nuclear chromatin. In group II the predominant lymphocytes were large with abundant cytoplasm. Group III was characterized by a heterogeneous population of lymphocytes with characteristics of both groups I and II. Clinical features of the patients were studied, and B and T typing of the lymphocytes was done. The median survival in group I was 26+ months; in group II 46+ months; and in group III 50+ months. Our data are at variance with previous reports and suggest that survival in patients with large lymphocytes is longer than in those with small lymphocytes.", "contents": "Morphology of chronic lymphocytic leukemia and its relationship to survival. The morphology of lymphocytes in blood and bone marrow from patients with chronic lymphocytic leukemia was studied; blood lymphocyte morphology was related to survival. Three primary morphologic groups emerged. Group 1 was characterized by small to medium-sized lymphocytes with narrow rims of cytoplasm and coarsely clumped nuclear chromatin. In group II the predominant lymphocytes were large with abundant cytoplasm. Group III was characterized by a heterogeneous population of lymphocytes with characteristics of both groups I and II. Clinical features of the patients were studied, and B and T typing of the lymphocytes was done. The median survival in group I was 26+ months; in group II 46+ months; and in group III 50+ months. Our data are at variance with previous reports and suggest that survival in patients with large lymphocytes is longer than in those with small lymphocytes."}
{"id": "PMID:1080633", "title": "New diagnostic pseudoisochromatic plates for dichromats based on subtractive color matches.", "content": "Further subtractive color matches obtained with the Lovibond Tintometer have confirmed the convergence data for dichromats obtained in a previous study. The characteristic color confusions obtained for 15 protanopes and 15 deuteranopes have been used in the selection of pairs of inks for use in the production of new screen-printed pseudoisochromatic plates incorporating geometric symbols and minimum form parameters. The success of these diagnostic plates is found to depend both on the careful selection of the inks to lie upon appropriate pseudoisochromatic lines but also on the reflected luminance contrast between the symbol and the background matrix of dots.", "contents": "New diagnostic pseudoisochromatic plates for dichromats based on subtractive color matches. Further subtractive color matches obtained with the Lovibond Tintometer have confirmed the convergence data for dichromats obtained in a previous study. The characteristic color confusions obtained for 15 protanopes and 15 deuteranopes have been used in the selection of pairs of inks for use in the production of new screen-printed pseudoisochromatic plates incorporating geometric symbols and minimum form parameters. The success of these diagnostic plates is found to depend both on the careful selection of the inks to lie upon appropriate pseudoisochromatic lines but also on the reflected luminance contrast between the symbol and the background matrix of dots."}
{"id": "PMID:1080634", "title": "Alteratons in RNA synthesis in lymphotoxin-treated target cells.", "content": "Lymphotoxin, a cellular mediator produced in vitro by antigen- or mitogen-stimulated lymphocytes, is capable of lysing various types of cells. We have shown that highly purified lymphotoxin preparations both mediate cytolysis and stimulate RNA synthesis in target cells. When RNA extracts from lymphotoxin-treated cells were examined by means of sucrose density gradient centrifugation, we observed a general increase in RNA synthesis, accentuated in species larger than 28S. Analysis on oligo(dT)-cellulose columns revealed a relatively larger increase in adenine-rich m-RNA. In concordance with this finding, we observed a moderate increase in RNA polymerase I (nucleolar) activity and a striking increase in RNA polymerase II (nucleoplasmic) activity in isolated nuclei of lymphotoxin-treated target cells.", "contents": "Alteratons in RNA synthesis in lymphotoxin-treated target cells. Lymphotoxin, a cellular mediator produced in vitro by antigen- or mitogen-stimulated lymphocytes, is capable of lysing various types of cells. We have shown that highly purified lymphotoxin preparations both mediate cytolysis and stimulate RNA synthesis in target cells. When RNA extracts from lymphotoxin-treated cells were examined by means of sucrose density gradient centrifugation, we observed a general increase in RNA synthesis, accentuated in species larger than 28S. Analysis on oligo(dT)-cellulose columns revealed a relatively larger increase in adenine-rich m-RNA. In concordance with this finding, we observed a moderate increase in RNA polymerase I (nucleolar) activity and a striking increase in RNA polymerase II (nucleoplasmic) activity in isolated nuclei of lymphotoxin-treated target cells."}
{"id": "PMID:1080635", "title": "Optically induced eye torsion. II. Optostatic and optokinetic cycloversion.", "content": "Using a synoptophore and an objective photographic method, it was demonstrated that the eyes undergo a tonic cycloversion under the influence of a pattern with slanting stripes. A slight tilt with respect to the vertical had a greater effect than a tilt with respect to the horizontal. The maximum amplitude was of the order of 1 degree. This optostatic eye torsion is considered to be the optomotor analogue of the \"ophthalmostatic\" otolithic reflex. Optokinetic torsion was also investigated. To this end, rotating discs that were divided into sectors were presented in a synoptophore. The maximum amplitude observed in the 4 subjects was 6 degrees. Optokinetic torsion may be viewed as the optical superstructure above the semicircular canal reflex. Non-fusional cyclovergence, for which purpose counter-inclined or counter rotating contours were presented to both eyes, could not be demonstrated with certainty.", "contents": "Optically induced eye torsion. II. Optostatic and optokinetic cycloversion. Using a synoptophore and an objective photographic method, it was demonstrated that the eyes undergo a tonic cycloversion under the influence of a pattern with slanting stripes. A slight tilt with respect to the vertical had a greater effect than a tilt with respect to the horizontal. The maximum amplitude was of the order of 1 degree. This optostatic eye torsion is considered to be the optomotor analogue of the \"ophthalmostatic\" otolithic reflex. Optokinetic torsion was also investigated. To this end, rotating discs that were divided into sectors were presented in a synoptophore. The maximum amplitude observed in the 4 subjects was 6 degrees. Optokinetic torsion may be viewed as the optical superstructure above the semicircular canal reflex. Non-fusional cyclovergence, for which purpose counter-inclined or counter rotating contours were presented to both eyes, could not be demonstrated with certainty."}
{"id": "PMID:1080636", "title": "The influence of topically applied bethanidine on intraocular pressure and pupil in the rabbit.", "content": "Bethanidine sulphate was administered topically to the eyes of conscious rabbits using a 0.5%, 1%, 2% and 5% solution of the drug. The intraocular pressures and pupil sizes were measured in the right treated and left control eyes at 0,1,2,3, 6 and 24 hrs. With all concentrations a pressure decrease with a maximum between 3 and 6 hrs after topical treatment occured. The 5% solution of bethanidine sulphate showed a delayed effect which was submaximal when compared to the 2% bethanidine solution. Using the 1% and 2% solution a ppressure decrease probably due to systemic action of the drug happened in the untreated control eyes. In all experiments the pressure responses had disappeared after 24 hrs in the treated and untreated eyes respectively. By means of the experimental conditions in this study no significant pupillary reactions were found after bethanidine application. The tension effect of 0.5% adrenaline borate remained essentially unchanged when repeated with additional 1% topical bethanidine. Some aspects of sympatholytic drug action on the intraocular pressure in the rabbit are discussed.", "contents": "The influence of topically applied bethanidine on intraocular pressure and pupil in the rabbit. Bethanidine sulphate was administered topically to the eyes of conscious rabbits using a 0.5%, 1%, 2% and 5% solution of the drug. The intraocular pressures and pupil sizes were measured in the right treated and left control eyes at 0,1,2,3, 6 and 24 hrs. With all concentrations a pressure decrease with a maximum between 3 and 6 hrs after topical treatment occured. The 5% solution of bethanidine sulphate showed a delayed effect which was submaximal when compared to the 2% bethanidine solution. Using the 1% and 2% solution a ppressure decrease probably due to systemic action of the drug happened in the untreated control eyes. In all experiments the pressure responses had disappeared after 24 hrs in the treated and untreated eyes respectively. By means of the experimental conditions in this study no significant pupillary reactions were found after bethanidine application. The tension effect of 0.5% adrenaline borate remained essentially unchanged when repeated with additional 1% topical bethanidine. Some aspects of sympatholytic drug action on the intraocular pressure in the rabbit are discussed."}
{"id": "PMID:1080637", "title": "Neonatal herpes simplex retinitis.", "content": "Pathologic material from an acute case of neonatal herpes simplex retinitis is presented. There was marked necrosis of all retinal layers including the pigment epithelium. Choroid and vitreous appeared only secondarily involved. The retinal necrosis was sharply delineated stopping at the ora serrata. We interpret this as being more compatible with an immunologically determined process than with direct infection. Indirect evidence for this viewpoint is the failure to find any evidence of virus in the retina, such as inclusion bodies, by electron microscopy or by fluorescent antibody staining.", "contents": "Neonatal herpes simplex retinitis. Pathologic material from an acute case of neonatal herpes simplex retinitis is presented. There was marked necrosis of all retinal layers including the pigment epithelium. Choroid and vitreous appeared only secondarily involved. The retinal necrosis was sharply delineated stopping at the ora serrata. We interpret this as being more compatible with an immunologically determined process than with direct infection. Indirect evidence for this viewpoint is the failure to find any evidence of virus in the retina, such as inclusion bodies, by electron microscopy or by fluorescent antibody staining."}
{"id": "PMID:1080638", "title": "Activated human retinal microglia under pathological conditions.", "content": "Six pathological human retinas were studied in order to establish changes, migration, and other reactions of retinal microglia. The different morphological features of other retinal macrophages are also described. Hortega silver carbonate stain was used for this study. This investigation aided in answering some questions on the active movements of microglia elements and other details under discussion.", "contents": "Activated human retinal microglia under pathological conditions. Six pathological human retinas were studied in order to establish changes, migration, and other reactions of retinal microglia. The different morphological features of other retinal macrophages are also described. Hortega silver carbonate stain was used for this study. This investigation aided in answering some questions on the active movements of microglia elements and other details under discussion."}
{"id": "PMID:1080639", "title": "[The proof of different types of collagen in the bovine eye (author's transl)].", "content": "Characteristically stained polyacrylamide gels can be obtained by disk-electrophoresis in acid medium of several tissues of the bovine eye. The technique permits to prove different types of collagen in the eye, and allows the differentiation and identification of the tissues. By these results the different tissues of the eye can be divided into three groups. 1. Tissues showing two alpha collagen components in polyacrylamide gel (cornea, sclera, iris, ciliary body, anterior lens capsule, and the pigmented epithelium of the retina). 2. Tissues possessing one alpha component only (zonula fiber and vitreous body). 3. Tissues which show neither the alpha nor the beta and gamma component of collagen (lens nucleous and retina without pigmented epithelium).", "contents": "[The proof of different types of collagen in the bovine eye (author's transl)]. Characteristically stained polyacrylamide gels can be obtained by disk-electrophoresis in acid medium of several tissues of the bovine eye. The technique permits to prove different types of collagen in the eye, and allows the differentiation and identification of the tissues. By these results the different tissues of the eye can be divided into three groups. 1. Tissues showing two alpha collagen components in polyacrylamide gel (cornea, sclera, iris, ciliary body, anterior lens capsule, and the pigmented epithelium of the retina). 2. Tissues possessing one alpha component only (zonula fiber and vitreous body). 3. Tissues which show neither the alpha nor the beta and gamma component of collagen (lens nucleous and retina without pigmented epithelium)."}
{"id": "PMID:1080640", "title": "Subconjunctival injection of antilymphocyte serum during corneal hypersensitivity to heterologous alubmin.", "content": "Thirty-two rabbits were immunized by subcutaneous human serum albumin in Freund's adjuvant and challenged after four weeks by intracorneal injection in both eyes. On a random and blind basis, one of the eyes in each animal received three consecutive injections of antilymphocyte serum obtained from rats and the other eye the same dose of normal rat serum. Statistical analysis of results showed no significant difference between both treatments. Two eyes treated with antilymphocyte serum had limbal necrosis with uveal prolapse.", "contents": "Subconjunctival injection of antilymphocyte serum during corneal hypersensitivity to heterologous alubmin. Thirty-two rabbits were immunized by subcutaneous human serum albumin in Freund's adjuvant and challenged after four weeks by intracorneal injection in both eyes. On a random and blind basis, one of the eyes in each animal received three consecutive injections of antilymphocyte serum obtained from rats and the other eye the same dose of normal rat serum. Statistical analysis of results showed no significant difference between both treatments. Two eyes treated with antilymphocyte serum had limbal necrosis with uveal prolapse."}
{"id": "PMID:1080641", "title": "Penetration of amikacin into the aphakic eye.", "content": "An experiment was designed to see if an aminoglycoside antibiotic, amikacin, would penetrate the vitreous of rabbits in greater concentrations in the postoperative aphakic eye than in the normal eye. Phakic and aphakic eyes were treated with amikacin by topical, subconjunctival, and intramuscular methods separately and combined. Bioassays of aqueous, anterior vitreous, and posterior vitreous revealed that concentrations of amikacin in ocular fluids after intramuscular administration were greater in the aphakic eye as compared to the normal eye; however, concentrations in the vitreous did not reach reliably bactericidal concentrations even when all three methods of administration were combined.", "contents": "Penetration of amikacin into the aphakic eye. An experiment was designed to see if an aminoglycoside antibiotic, amikacin, would penetrate the vitreous of rabbits in greater concentrations in the postoperative aphakic eye than in the normal eye. Phakic and aphakic eyes were treated with amikacin by topical, subconjunctival, and intramuscular methods separately and combined. Bioassays of aqueous, anterior vitreous, and posterior vitreous revealed that concentrations of amikacin in ocular fluids after intramuscular administration were greater in the aphakic eye as compared to the normal eye; however, concentrations in the vitreous did not reach reliably bactericidal concentrations even when all three methods of administration were combined."}
{"id": "PMID:1080642", "title": "Photocoagulation of angioma of the eyelid.", "content": "An infant with a large hemangioma (strawberry mark with a cavernous component) of the eyelid was successfully treated with photocoagulation. The method resulted in rapid involution of the lesion and the visual function of the involved eye could be preserved. A similar treatment in a case with strawberry mark and another with pigmented nevus in the eyelid brought about excellent cosmetic results.", "contents": "Photocoagulation of angioma of the eyelid. An infant with a large hemangioma (strawberry mark with a cavernous component) of the eyelid was successfully treated with photocoagulation. The method resulted in rapid involution of the lesion and the visual function of the involved eye could be preserved. A similar treatment in a case with strawberry mark and another with pigmented nevus in the eyelid brought about excellent cosmetic results."}
{"id": "PMID:1080643", "title": "Ultrastructural features of posterior vitreous detachment.", "content": "The ultrastructural features of vitreoretinal relations in the posterior fundus of enucleated eyes were studied. Vitreous detachment occurs at the anatomical vitreoretinal juncture, between the inner limiting lamina of the retina and the vitreous cortex, the surface of the lamina becoming smooth and without vitreous residues.", "contents": "Ultrastructural features of posterior vitreous detachment. The ultrastructural features of vitreoretinal relations in the posterior fundus of enucleated eyes were studied. Vitreous detachment occurs at the anatomical vitreoretinal juncture, between the inner limiting lamina of the retina and the vitreous cortex, the surface of the lamina becoming smooth and without vitreous residues."}
{"id": "PMID:1080644", "title": "Histopathology after vitrectomy with the vitrophage.", "content": "A 72-year-old adult-onset diabetic woman underwent vitrectomy with the vitrophage because of persistent vitreous hemorrhage. Postoperatively a hyphema developed and elevation of intraocular pressure was refractory to medical management. Intraocular pressure was controllable within normal limits after vitreous lavage. Eleven days after vitreous lavage the patient died from pulmonary embolization. Histologic sections demonstrated a flat pars plana over the sclerotomy site with vitreous condensation along the internal aspect of the wound. Rubeosis iridis was postulated as the source of the hyphema. A hemolytic mechanism may have caused the elevated intraocular pressure.", "contents": "Histopathology after vitrectomy with the vitrophage. A 72-year-old adult-onset diabetic woman underwent vitrectomy with the vitrophage because of persistent vitreous hemorrhage. Postoperatively a hyphema developed and elevation of intraocular pressure was refractory to medical management. Intraocular pressure was controllable within normal limits after vitreous lavage. Eleven days after vitreous lavage the patient died from pulmonary embolization. Histologic sections demonstrated a flat pars plana over the sclerotomy site with vitreous condensation along the internal aspect of the wound. Rubeosis iridis was postulated as the source of the hyphema. A hemolytic mechanism may have caused the elevated intraocular pressure."}
{"id": "PMID:1080645", "title": "[Signs, therapy and follow-up of cases with pure blow-out fractures of the orbit (author's transl)].", "content": "30 patients with blow-out fractures were referred to our clinic because of serious \"eye troubles\", most frequently diplopia and haematoma of the eye lids (Table 1). The signs of the characteristic oculomotor imbalance are described. In 10 out of 29 patients motility was normalised spontaneously within 2 weeks. 19 patients underwent surgery because one of the following criteria was fulfilled: 1. Diplopia and positive traction-test not resolving within 10 days. 2. Large hermiation into the antrum. We believe, that posttraumatic diplopia is not only a result of the incarceration, but also of a direct laesion of muscles and connective tissue. Surgery was done by orbital approach. After reposition of antral prolaps and the bony fragment we used Lyodura or, when nessessary, silicon implants. In all patients treated in this way ocular motility was improved, but normalisation was achieved only in 8 cases (Fig. 8).", "contents": "[Signs, therapy and follow-up of cases with pure blow-out fractures of the orbit (author's transl)]. 30 patients with blow-out fractures were referred to our clinic because of serious \"eye troubles\", most frequently diplopia and haematoma of the eye lids (Table 1). The signs of the characteristic oculomotor imbalance are described. In 10 out of 29 patients motility was normalised spontaneously within 2 weeks. 19 patients underwent surgery because one of the following criteria was fulfilled: 1. Diplopia and positive traction-test not resolving within 10 days. 2. Large hermiation into the antrum. We believe, that posttraumatic diplopia is not only a result of the incarceration, but also of a direct laesion of muscles and connective tissue. Surgery was done by orbital approach. After reposition of antral prolaps and the bony fragment we used Lyodura or, when nessessary, silicon implants. In all patients treated in this way ocular motility was improved, but normalisation was achieved only in 8 cases (Fig. 8)."}
{"id": "PMID:1080646", "title": "[The sources of ophthalmodynamography. Determination of tissue volumes of human lids and orbital contents (author's transl)].", "content": "The volumetric determination of all those tissues relevant for Opthalmodynamography (ODG) showed the lids to contribute about a quarter to the total volume; another quarter each was due to the optic bulb including optic fascicel, external bulbar musculature and orbital fat. Bulb and fascicel show no pulsations that could be detected oscillographically, fat tissue is poorly vascularized and therefore is of minor importance in respect to the origin of pulsation. The main causes of ODG-oscillations therefore are external eye muscels and eye lids, each contributing a minimum of one third to the heights of the pulse. Assuming all tissues of possible importance for the origin of ODG to be equally well vascularized the part of ODG pulse height due to influence of eye lids is minimally 25% or 32% in the average.", "contents": "[The sources of ophthalmodynamography. Determination of tissue volumes of human lids and orbital contents (author's transl)]. The volumetric determination of all those tissues relevant for Opthalmodynamography (ODG) showed the lids to contribute about a quarter to the total volume; another quarter each was due to the optic bulb including optic fascicel, external bulbar musculature and orbital fat. Bulb and fascicel show no pulsations that could be detected oscillographically, fat tissue is poorly vascularized and therefore is of minor importance in respect to the origin of pulsation. The main causes of ODG-oscillations therefore are external eye muscels and eye lids, each contributing a minimum of one third to the heights of the pulse. Assuming all tissues of possible importance for the origin of ODG to be equally well vascularized the part of ODG pulse height due to influence of eye lids is minimally 25% or 32% in the average."}
{"id": "PMID:1080647", "title": "[Evidence and meaning of monophasic potentials in the electromyogram of extraocular muscles of rabbits and cats (author's transl)].", "content": "The EMG of extraocular muscles of rabbits and cats was recorded by a concentric-needle electrode provided for clinical use. The EMG showed spike activity (at least biphasic, fast waves of 1 to 2 msec duration) and characteristic long-duration monophasic waves (decline for up to 7 msec). The monophasic potentials are interpreted as local depolarizations arising at the nerve-muscle junctions of multiply innervated muscle fibers. Spike- and monophasic potentials are discharged in independent rhythms whose frequency changes when the head position is altered. The frequency of spike- and monophasic potentials are changed in the same direction by labyrinthine reflexes. Spontaneous monophasic potentials recruit at a lower threshold.", "contents": "[Evidence and meaning of monophasic potentials in the electromyogram of extraocular muscles of rabbits and cats (author's transl)]. The EMG of extraocular muscles of rabbits and cats was recorded by a concentric-needle electrode provided for clinical use. The EMG showed spike activity (at least biphasic, fast waves of 1 to 2 msec duration) and characteristic long-duration monophasic waves (decline for up to 7 msec). The monophasic potentials are interpreted as local depolarizations arising at the nerve-muscle junctions of multiply innervated muscle fibers. Spike- and monophasic potentials are discharged in independent rhythms whose frequency changes when the head position is altered. The frequency of spike- and monophasic potentials are changed in the same direction by labyrinthine reflexes. Spontaneous monophasic potentials recruit at a lower threshold."}
{"id": "PMID:1080648", "title": "In vitro estrone-estradiol-17beta interconversion in the cornea, lens, iris and retina of the rabbit eye.", "content": "Estrone and estradiol-17beta were incubated with various eye tissues of female rabbits. The interconversion of both estrogenes by the action of 17beta-hydroxysteroid dehydrogenase decreased in the sequence cornea, iris, retina, and lens. In a smaller yield estradiol-17alpha was also formed from estrone. The cornea seems to be an active estrogen-metabolizing tissue. The role of estrogens and progestogens in the tissues of the anterior eye segment is briefly discussed.", "contents": "In vitro estrone-estradiol-17beta interconversion in the cornea, lens, iris and retina of the rabbit eye. Estrone and estradiol-17beta were incubated with various eye tissues of female rabbits. The interconversion of both estrogenes by the action of 17beta-hydroxysteroid dehydrogenase decreased in the sequence cornea, iris, retina, and lens. In a smaller yield estradiol-17alpha was also formed from estrone. The cornea seems to be an active estrogen-metabolizing tissue. The role of estrogens and progestogens in the tissues of the anterior eye segment is briefly discussed."}
{"id": "PMID:1080651", "title": "Physical properties of sputum. VII. Rheologic properties and mucociliary transport.", "content": "Sputums from bronchitic patients were measured for viscosity and elastic recoil and then placed on an isolated frog pharyngeal palate to study the velocity of transport by beating mucosal cilia. Purulent sputum, as a group, demonstrated a higher viscosity and a lower elastic recoil, and was transported at a slower rate than mucoid samples of low viscosity and high recoil. Indigenous frog mucus, collected and reapplied to the palate, had the lowest viscosity, the highest elastic recoil, and the fastest transport rate. Thus, it was concluded that high elastic recoil and low viscosity represent the best rheologic combination for maximal velocity by a mucociliary system.", "contents": "Physical properties of sputum. VII. Rheologic properties and mucociliary transport. Sputums from bronchitic patients were measured for viscosity and elastic recoil and then placed on an isolated frog pharyngeal palate to study the velocity of transport by beating mucosal cilia. Purulent sputum, as a group, demonstrated a higher viscosity and a lower elastic recoil, and was transported at a slower rate than mucoid samples of low viscosity and high recoil. Indigenous frog mucus, collected and reapplied to the palate, had the lowest viscosity, the highest elastic recoil, and the fastest transport rate. Thus, it was concluded that high elastic recoil and low viscosity represent the best rheologic combination for maximal velocity by a mucociliary system."}
{"id": "PMID:1080656", "title": "Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics.", "content": "Ampicillin-resistant and -susceptible strains of Haemophilus influenzae were tested for susceptibility to 16 antibiotics. Chloramphenicol and a new cephalosporin, cefamandole, were most active with minimal inhibitory concentrations (MICs) for all bacteria tested between 0.5 to 2.0 mug/ml. All but two organisms were susceptible to tetracycline. Ampicillin-resistant strains of H. influenzae were less susceptible (MIC, 4 to 32 mug/ml) to carbenicillin and ticarcillin than ampicillin-susceptible organisms (MIC, 0.25 to 1.0 mug/ml). A rapid assay for beta-lactamase, utilizing a chromogenic cephalosporin substrate, detected enzyme production in all 17 ampicillin-resistant strains of H. influenzae.", "contents": "Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics. Ampicillin-resistant and -susceptible strains of Haemophilus influenzae were tested for susceptibility to 16 antibiotics. Chloramphenicol and a new cephalosporin, cefamandole, were most active with minimal inhibitory concentrations (MICs) for all bacteria tested between 0.5 to 2.0 mug/ml. All but two organisms were susceptible to tetracycline. Ampicillin-resistant strains of H. influenzae were less susceptible (MIC, 4 to 32 mug/ml) to carbenicillin and ticarcillin than ampicillin-susceptible organisms (MIC, 0.25 to 1.0 mug/ml). A rapid assay for beta-lactamase, utilizing a chromogenic cephalosporin substrate, detected enzyme production in all 17 ampicillin-resistant strains of H. influenzae."}
{"id": "PMID:1080658", "title": "Modification of postirradiation hydroproteolytic activity of rat's pancreas by some drugs.", "content": "Influence of Trasylol and morphine on hydroproteolytic activity of rat's pancreas was investigated. Trasylol, administered in vivo, caused normalization of activity of some pancreatic enzymes in the rats single and fractionated irradiated. In vitro, however, Trasylol did not affect the catheptic (pH 5.4) and amylolytic activity of the pancreas. After in vivo administration of morphine, the return to control values of amylolytic activity in the serum of single irradiated animals was observed.", "contents": "Modification of postirradiation hydroproteolytic activity of rat's pancreas by some drugs. Influence of Trasylol and morphine on hydroproteolytic activity of rat's pancreas was investigated. Trasylol, administered in vivo, caused normalization of activity of some pancreatic enzymes in the rats single and fractionated irradiated. In vitro, however, Trasylol did not affect the catheptic (pH 5.4) and amylolytic activity of the pancreas. After in vivo administration of morphine, the return to control values of amylolytic activity in the serum of single irradiated animals was observed."}
{"id": "PMID:1080659", "title": "A rehabilitation evaluation system which complements the problem-oriented medical record.", "content": "The traditional medical history and physical examination format is disease rather than disability oriented. It has been shown to be incomplete for the total evaluation of rehabilitation patients. Direct applications of Weed's Problem-Oriented Medical Record have proven to be formidable and cumbersome due to the complexity and diversity of rehabilitation. Therefore, we have developed the Rehabilitation Evaluation System (RES) to document functional rehabilitation management and progress during inpatient hospitalization and outpatient follow-up. The system identifies 18 key rehabilitation areas, each with an individual and objective four-point scale. Utilization of this system in our department has been invaluable in formulating goals and continually evaluating the on-going rehabilitation process. We used the RES with equal facility on 46 rehabilitation inpatients including stroke, amputation, spinal cord injury, multiple sclerosis, orthopedic-trauma, rheumatoid arthritis and poliomyelitis. The mechanics of the RES are presented in detail with a specific patient-example of hemiplegia. Its complimentary use with the Problem-Oriented Medical Record is discussed. Practical advantages are seen in patient care, medical student and resident education, record keeping and research.", "contents": "A rehabilitation evaluation system which complements the problem-oriented medical record. The traditional medical history and physical examination format is disease rather than disability oriented. It has been shown to be incomplete for the total evaluation of rehabilitation patients. Direct applications of Weed's Problem-Oriented Medical Record have proven to be formidable and cumbersome due to the complexity and diversity of rehabilitation. Therefore, we have developed the Rehabilitation Evaluation System (RES) to document functional rehabilitation management and progress during inpatient hospitalization and outpatient follow-up. The system identifies 18 key rehabilitation areas, each with an individual and objective four-point scale. Utilization of this system in our department has been invaluable in formulating goals and continually evaluating the on-going rehabilitation process. We used the RES with equal facility on 46 rehabilitation inpatients including stroke, amputation, spinal cord injury, multiple sclerosis, orthopedic-trauma, rheumatoid arthritis and poliomyelitis. The mechanics of the RES are presented in detail with a specific patient-example of hemiplegia. Its complimentary use with the Problem-Oriented Medical Record is discussed. Practical advantages are seen in patient care, medical student and resident education, record keeping and research."}
{"id": "PMID:1080661", "title": "Unsuspected intravestibular schwannoma.", "content": "The intravestibular schwannoma is physically minute and will often elude preoperative diagnosis. From time to time, such a tumor will be unexpectedly encountered during transcanal labyrinthectomy. A review of pathologic and surgical literature suggests that the lesion can be well managed by the transcanal route. A standard labyrinthectomy may be sufficient, but the translabyrinthine approach may be necessary for more extensive tumors.", "contents": "Unsuspected intravestibular schwannoma. The intravestibular schwannoma is physically minute and will often elude preoperative diagnosis. From time to time, such a tumor will be unexpectedly encountered during transcanal labyrinthectomy. A review of pathologic and surgical literature suggests that the lesion can be well managed by the transcanal route. A standard labyrinthectomy may be sufficient, but the translabyrinthine approach may be necessary for more extensive tumors."}
{"id": "PMID:1080662", "title": "A case of lymphoma with T-cell characteristics.", "content": "This case report concerns a woman of 54 who died from a rapidly progressive lymphosarcoma-cell leukaemia with the terminal complication of toxoplasmosis.  The grossly atypical lymphosarcoma cells were shown to form rosettes with sheep erythrocytes but were incapable of responding to stimulation with phytohaemagglutinin.  They thus had the functional characteristics of abnormal T-cells.  The proportion of immunoglobulin-bearing lymphocytes was greatly reduced.", "contents": "A case of lymphoma with T-cell characteristics. This case report concerns a woman of 54 who died from a rapidly progressive lymphosarcoma-cell leukaemia with the terminal complication of toxoplasmosis.  The grossly atypical lymphosarcoma cells were shown to form rosettes with sheep erythrocytes but were incapable of responding to stimulation with phytohaemagglutinin.  They thus had the functional characteristics of abnormal T-cells.  The proportion of immunoglobulin-bearing lymphocytes was greatly reduced."}
{"id": "PMID:1080663", "title": "Some effects of alcohol on various aspects of oculomotor control.", "content": "Recent studies have shown that alcohol interferes with visual control of vestibular nystagmus. The present study was designed to assess three partially independent systems of oculomotor control. Performance on three tasks was measured before and after mild alcohol dosage. One task involved visual suppression of vestibular nystagmus; a second involved smooth oculomotor tracking of a moving target; and a third required repetitive rapid voluntary shifts in gaze. Oculomotor control was degraded on the first two tasks with recovery toward the initial performance level 4 h after drinking. Performance on the third task was not obviously degraded, although it is possible that improvement with practice was retarded. Results are discussed in terms of neurological systems involved and kinds of flight tasks potentially affected.", "contents": "Some effects of alcohol on various aspects of oculomotor control. Recent studies have shown that alcohol interferes with visual control of vestibular nystagmus. The present study was designed to assess three partially independent systems of oculomotor control. Performance on three tasks was measured before and after mild alcohol dosage. One task involved visual suppression of vestibular nystagmus; a second involved smooth oculomotor tracking of a moving target; and a third required repetitive rapid voluntary shifts in gaze. Oculomotor control was degraded on the first two tasks with recovery toward the initial performance level 4 h after drinking. Performance on the third task was not obviously degraded, although it is possible that improvement with practice was retarded. Results are discussed in terms of neurological systems involved and kinds of flight tasks potentially affected."}
{"id": "PMID:1080664", "title": "Visualization of surface topology of human lymphoid cells by scanning electron microscopy.", "content": "We have studied the surface characteristics of human lymphoid cells with the scanning electron microscope (SEM). We obtained thymic cells by mincing thymus and isolating peripheral blood lymphocytes from normal donors as well as patients with acute and chronic lymphocytic leukemia (ALL and CLL). Prior to SEM, we purified the lymphocytes in isoosmotic Ficoll-Hypaque gradients. In addition, we examined several lymphoid cell lines in permanent culture derived from normal persons, patients with infectious mononucleosis, as well as from patients with ALL. The surfaces of most thymic cells appear smooth with only a few short microvilli. A small number of thymic cells, on the other hand, are found to exhibit many more microvilli of uniform shape and length. We could further identify lymphocytes from normal donors as T- and B-types according to their rosetting spontaneously with sheep red blood cells (SRBC) resemble thymic cells in their surface topology. However, B-cells rosetting with amboceptor--and complement--treated SRBC show greater number of microvilli. The surface morphology of lymphocytes from leukemic patients appears more or less characteristic of the disease type. In contrast, the surfaces of cultured lymphoid cells show a diversified surface topology, often with large smooth areas, very long and very short microcilli, which may often cluster in groups. These data bear directly upon techniques of plasma membrane fractionation and we suspect that the topology differences correlate with their differentiation patterns.", "contents": "Visualization of surface topology of human lymphoid cells by scanning electron microscopy. We have studied the surface characteristics of human lymphoid cells with the scanning electron microscope (SEM). We obtained thymic cells by mincing thymus and isolating peripheral blood lymphocytes from normal donors as well as patients with acute and chronic lymphocytic leukemia (ALL and CLL). Prior to SEM, we purified the lymphocytes in isoosmotic Ficoll-Hypaque gradients. In addition, we examined several lymphoid cell lines in permanent culture derived from normal persons, patients with infectious mononucleosis, as well as from patients with ALL. The surfaces of most thymic cells appear smooth with only a few short microvilli. A small number of thymic cells, on the other hand, are found to exhibit many more microvilli of uniform shape and length. We could further identify lymphocytes from normal donors as T- and B-types according to their rosetting spontaneously with sheep red blood cells (SRBC) resemble thymic cells in their surface topology. However, B-cells rosetting with amboceptor--and complement--treated SRBC show greater number of microvilli. The surface morphology of lymphocytes from leukemic patients appears more or less characteristic of the disease type. In contrast, the surfaces of cultured lymphoid cells show a diversified surface topology, often with large smooth areas, very long and very short microcilli, which may often cluster in groups. These data bear directly upon techniques of plasma membrane fractionation and we suspect that the topology differences correlate with their differentiation patterns."}
{"id": "PMID:1080668", "title": "Studies on the effector cell of anti-tumour immunity in a chemically induced mouse tumour system.", "content": "Spleen cells from mice immunized against a methylcholanthrene induced rhabdomyosarcoma inhibited tumour cell colony formation in vitro and prevented tumour development in vivo in an adoptive transfer test. Treatment of the immune spleen cells with anti-mouse immunoglobulin serum or passage through a nylon wool column, both of which reduced the percentage of immunoglobulin bearing cells in the population to less than 3-4%, did not alter their anti-tumour effects. In contrast, treatment of the spleen cells with anti-BAomicron serum abolished their anti-tumour effects both in vitro and in vivo. These results indicate that T cells are the mediators of tumour immunity in this chemically induced tumour system.", "contents": "Studies on the effector cell of anti-tumour immunity in a chemically induced mouse tumour system. Spleen cells from mice immunized against a methylcholanthrene induced rhabdomyosarcoma inhibited tumour cell colony formation in vitro and prevented tumour development in vivo in an adoptive transfer test. Treatment of the immune spleen cells with anti-mouse immunoglobulin serum or passage through a nylon wool column, both of which reduced the percentage of immunoglobulin bearing cells in the population to less than 3-4%, did not alter their anti-tumour effects. In contrast, treatment of the spleen cells with anti-BAomicron serum abolished their anti-tumour effects both in vitro and in vivo. These results indicate that T cells are the mediators of tumour immunity in this chemically induced tumour system."}
{"id": "PMID:1080669", "title": "The enhancing influence of proteolysis on E rosette forming lymphocytes (T cells) in vivo and in vitro.", "content": "The T lymphocytes populations of 22 young healthy adults, 21 healthy middle aged and older blood donors, 35 non-pregnant women of child bearing age and 14 patients with advanced malignant disease were assessed and compared. It was found that the mean T cell counts in the middle aged and older controls were significantly lower than in the healthy young adults and were further reduced in the patients with malignant disease. The addition of the proteolytic agent brinase (protease 1 obtained from Aspergillus oryzae) to the rosetting test increased the T cell counts signficantly in all groups. This was mot marked in the older age groups and the patients with malignant disease. The proteolytic agent is shown to exert its effect on the lymphocytes in the test. Slow intravenous infusion of either brinase or streptokinase into patients with malignant disease is shown to result in increased T lymphocyte counts pari passu with a restoration of skin allergy. The significance of these findings and possible mode of action of the proteolytic agents in increasing T cell activity are discussed.", "contents": "The enhancing influence of proteolysis on E rosette forming lymphocytes (T cells) in vivo and in vitro. The T lymphocytes populations of 22 young healthy adults, 21 healthy middle aged and older blood donors, 35 non-pregnant women of child bearing age and 14 patients with advanced malignant disease were assessed and compared. It was found that the mean T cell counts in the middle aged and older controls were significantly lower than in the healthy young adults and were further reduced in the patients with malignant disease. The addition of the proteolytic agent brinase (protease 1 obtained from Aspergillus oryzae) to the rosetting test increased the T cell counts signficantly in all groups. This was mot marked in the older age groups and the patients with malignant disease. The proteolytic agent is shown to exert its effect on the lymphocytes in the test. Slow intravenous infusion of either brinase or streptokinase into patients with malignant disease is shown to result in increased T lymphocyte counts pari passu with a restoration of skin allergy. The significance of these findings and possible mode of action of the proteolytic agents in increasing T cell activity are discussed."}
{"id": "PMID:1080671", "title": "Effect of local injection of Corynebacterium parvum on the growth of a murine fibrosarcoma.", "content": "Local injection (i.e. injection at the site of tumour inoculation) of strains of C. Parvum which have a significant anti-tumour effect when given systemically (i.e. intravenously or, in the case of subcutaneous tumour transplant, intraperitoneally) strongly inhibits the growth of isogeneic transplants of a fibrosarcoma in intact CBA mice but has little or no effect on subcutaneous transplants of the same tumour in T-cell deprived mice. The anti-tumour effect of local injection of C. parvum, unlike that of systemic injection in this particular tumour system, thus appears to be T-cell dependent.", "contents": "Effect of local injection of Corynebacterium parvum on the growth of a murine fibrosarcoma. Local injection (i.e. injection at the site of tumour inoculation) of strains of C. Parvum which have a significant anti-tumour effect when given systemically (i.e. intravenously or, in the case of subcutaneous tumour transplant, intraperitoneally) strongly inhibits the growth of isogeneic transplants of a fibrosarcoma in intact CBA mice but has little or no effect on subcutaneous transplants of the same tumour in T-cell deprived mice. The anti-tumour effect of local injection of C. parvum, unlike that of systemic injection in this particular tumour system, thus appears to be T-cell dependent."}
{"id": "PMID:1080672", "title": "Stimulation of granulocytic colony formation  in agar diffusion chambers implanted in cyclophosphamide pretreated mice.", "content": "The growth of mouse bone marrow colonies in agar diffusion chambers (ADCs) was evaluated using host mice injected with saline or with cyclophosphamide (CY) before chamber insertion. The mice pretreated with cyclophosphamide proved more effective hosts than control (saline pretreated) mice, indicating that cyclophosphamide causes the elaboration of a stimulating factor acting on colony precursor cells. Assays of the factor for colony stimulating activity against mouse bone marrow cells in agar culture in vitro suggest that potentiation may be due to a slight temporary increase in the level of colony stimulating factor (CSF) in the chamber environment, although a parallel increase was not detected in the serum. Stem cell recovery from the ADCs, measured by spleen colony formation, suggests that the stimulus may act by increasing differentiation at the level of the pluripotential stem cell.", "contents": "Stimulation of granulocytic colony formation  in agar diffusion chambers implanted in cyclophosphamide pretreated mice. The growth of mouse bone marrow colonies in agar diffusion chambers (ADCs) was evaluated using host mice injected with saline or with cyclophosphamide (CY) before chamber insertion. The mice pretreated with cyclophosphamide proved more effective hosts than control (saline pretreated) mice, indicating that cyclophosphamide causes the elaboration of a stimulating factor acting on colony precursor cells. Assays of the factor for colony stimulating activity against mouse bone marrow cells in agar culture in vitro suggest that potentiation may be due to a slight temporary increase in the level of colony stimulating factor (CSF) in the chamber environment, although a parallel increase was not detected in the serum. Stem cell recovery from the ADCs, measured by spleen colony formation, suggests that the stimulus may act by increasing differentiation at the level of the pluripotential stem cell."}
{"id": "PMID:1080673", "title": "Storage and metabolism of poly(adenylic acid)-mRNA in germinating cotton seeds.", "content": "When the RNA from both the nucleus and polysomes of germinated cotton seeds were fractionated on poly(U)-cellulose columns it was found that both contained poly(A)-RNA. In ungerminated seeds most of the poly(A)-RNA is located in the nucleus of the cell. 3'-Deoxyadenosine was found to inhibit the synthesis of both nuclear and polysomal poly(A) by 75% in germinated seeds, but had little or no effect on protein synthesis during the first 6 hr of germination. After this time 3'-deoxyadenosine had a pronounced inhibitory effect on protein synthesis presumably due to the inhibition of poly(A) addition to newly synthesized mRNA.", "contents": "Storage and metabolism of poly(adenylic acid)-mRNA in germinating cotton seeds. When the RNA from both the nucleus and polysomes of germinated cotton seeds were fractionated on poly(U)-cellulose columns it was found that both contained poly(A)-RNA. In ungerminated seeds most of the poly(A)-RNA is located in the nucleus of the cell. 3'-Deoxyadenosine was found to inhibit the synthesis of both nuclear and polysomal poly(A) by 75% in germinated seeds, but had little or no effect on protein synthesis during the first 6 hr of germination. After this time 3'-deoxyadenosine had a pronounced inhibitory effect on protein synthesis presumably due to the inhibition of poly(A) addition to newly synthesized mRNA."}
{"id": "PMID:1080674", "title": "The functional unit of electrical events and phosphorylation in chromatophores from Rhodopseudomonas sphaeroides.", "content": "1. From electron micrographs of chromatophores from Rhodopseudomonas spaeroides and from the estimated bacteriochlorophyll per chromatophore was estimated. The mean diameter of the chromatophore vesicles was 600 A. 2. The decay of the flash-induced electric potential across the chromatophore membrane measured by the carotenoid band shift was 20% accelerated by about one valinomycin molecule per 4700 bacteriochlorophyll, i.e. by one ionophore molecule per chromatophore. 3. The inhibition of the flash-induced ATP formation by valinomycin followed a similar pattern to the accelerated decay of the electric potential. 4. The single turnover flash yield of ATP synthesis gave a mean value of one ATP per 1470 bacteriochlorophyll or about 3 ATP per vesicle. 5. With regard to the partitioning of the ionophore between the membrane (85%) and aqueous phase (15%) we conclude that one molecule of valinomycin per chromatophore is sufficient to begin to collapse the electrical potential and inhibit ATP synthesis. It is therefore suggested that the membrane potential is an essential component of the energized state which is used for phosphorylation. The results correspond to those obtained for the 100-fold larger vesicles in chloroplasts (thylakoids) where one molecule of ioophore is also sufficient to quench both events.", "contents": "The functional unit of electrical events and phosphorylation in chromatophores from Rhodopseudomonas sphaeroides. 1. From electron micrographs of chromatophores from Rhodopseudomonas spaeroides and from the estimated bacteriochlorophyll per chromatophore was estimated. The mean diameter of the chromatophore vesicles was 600 A. 2. The decay of the flash-induced electric potential across the chromatophore membrane measured by the carotenoid band shift was 20% accelerated by about one valinomycin molecule per 4700 bacteriochlorophyll, i.e. by one ionophore molecule per chromatophore. 3. The inhibition of the flash-induced ATP formation by valinomycin followed a similar pattern to the accelerated decay of the electric potential. 4. The single turnover flash yield of ATP synthesis gave a mean value of one ATP per 1470 bacteriochlorophyll or about 3 ATP per vesicle. 5. With regard to the partitioning of the ionophore between the membrane (85%) and aqueous phase (15%) we conclude that one molecule of valinomycin per chromatophore is sufficient to begin to collapse the electrical potential and inhibit ATP synthesis. It is therefore suggested that the membrane potential is an essential component of the energized state which is used for phosphorylation. The results correspond to those obtained for the 100-fold larger vesicles in chloroplasts (thylakoids) where one molecule of ioophore is also sufficient to quench both events."}
{"id": "PMID:1080677", "title": "Treatment of life-threatening hemorrhage due to acquired factor VIII inhibitor.", "content": "An otherwise healthy elderly man developed massive, life-threatening, sublingual bleeding associated with an idiopathic factor VIII inhibitor. The patient was treated wtih cyclophosphamide, steroids, factor VIII concentrates, and repeated plasmapheresis (including three times with NCI-IBM blood-cell separator). Rapid clinical and laboratory improvement occurred, with complete disappearance of the inhibitor. The patient has remained well, without evidence of an inhibitor, for 8 mo. The possible role of each of the therapeutic measures in the disappearance of the inhibitor and the possible pathogenetic mechanism of this disorder are discussed. A high mortality rate and a striking incidence of sublingual hematoma have been observed in cases in the literature.", "contents": "Treatment of life-threatening hemorrhage due to acquired factor VIII inhibitor. An otherwise healthy elderly man developed massive, life-threatening, sublingual bleeding associated with an idiopathic factor VIII inhibitor. The patient was treated wtih cyclophosphamide, steroids, factor VIII concentrates, and repeated plasmapheresis (including three times with NCI-IBM blood-cell separator). Rapid clinical and laboratory improvement occurred, with complete disappearance of the inhibitor. The patient has remained well, without evidence of an inhibitor, for 8 mo. The possible role of each of the therapeutic measures in the disappearance of the inhibitor and the possible pathogenetic mechanism of this disorder are discussed. A high mortality rate and a striking incidence of sublingual hematoma have been observed in cases in the literature."}
{"id": "PMID:1080679", "title": "The effect of heparin on platelet aggregation by common inductors and by ristocetin in congenital bleeding disorders due to factor VIII or fibrinogen defects.", "content": "Platelet aggregation by various inductors was studied in citrated and heparinized plasma of the following groups of subjects: Normal, hemophilia A, combined factor V and factor VIII deficiency, v. Willeprand's disease and congenital afibrinognemia. The results may be summarized as follows: A-platelet aggregation in citrated plasm 1) platelet aggregation by common inductors ADP, adrenalin and collagen was normal in all groups of subjects but for the patients with congential afibrinogenemia in whom adrenalin induced aggregation was absent or markedly refuced whereas ADP and collagen gave slightly reduced or near normal aggregation curves. 2) platelet aggregation by ristocetin was normal in all groups of subjects but for v. Willebrand's disease in which it was absent. B-platelet aggregation in heparized plasma. 1) platelet aggregation by common inductors resulted to be normal in all groups of subjects except in congenital afibrinogenemia. In this latter case the pattern was still mildly defective but here was an increased aggregation as compared to citrated plasma. These findings have been interpretemmon inductors. 2) platelet aggregation by ristocetin resulted to be absent in all groups of subjects investigated. The possible mechanism of action of the inhibitory effect exercised py heparin with regard to restocetin is discussed.", "contents": "The effect of heparin on platelet aggregation by common inductors and by ristocetin in congenital bleeding disorders due to factor VIII or fibrinogen defects. Platelet aggregation by various inductors was studied in citrated and heparinized plasma of the following groups of subjects: Normal, hemophilia A, combined factor V and factor VIII deficiency, v. Willeprand's disease and congenital afibrinognemia. The results may be summarized as follows: A-platelet aggregation in citrated plasm 1) platelet aggregation by common inductors ADP, adrenalin and collagen was normal in all groups of subjects but for the patients with congential afibrinogenemia in whom adrenalin induced aggregation was absent or markedly refuced whereas ADP and collagen gave slightly reduced or near normal aggregation curves. 2) platelet aggregation by ristocetin was normal in all groups of subjects but for v. Willebrand's disease in which it was absent. B-platelet aggregation in heparized plasma. 1) platelet aggregation by common inductors resulted to be normal in all groups of subjects except in congenital afibrinogenemia. In this latter case the pattern was still mildly defective but here was an increased aggregation as compared to citrated plasma. These findings have been interpretemmon inductors. 2) platelet aggregation by ristocetin resulted to be absent in all groups of subjects investigated. The possible mechanism of action of the inhibitory effect exercised py heparin with regard to restocetin is discussed."}
{"id": "PMID:1080681", "title": "Acute segmental ischaemic enteritis in Thialand.", "content": "A disease characterized by segments of ischaemic small intestine has been recognized in norther Thailand over the past decade. The clinical features and appearance of the diseased intestime are described. Most of the patients were treated by surgical resection of the affected bowel. The overall mortality was 14 per cent. Recently, some patients have been successfully treated by non-surgical means. The aetiology of the disease is still unknown.", "contents": "Acute segmental ischaemic enteritis in Thialand. A disease characterized by segments of ischaemic small intestine has been recognized in norther Thailand over the past decade. The clinical features and appearance of the diseased intestime are described. Most of the patients were treated by surgical resection of the affected bowel. The overall mortality was 14 per cent. Recently, some patients have been successfully treated by non-surgical means. The aetiology of the disease is still unknown."}
{"id": "PMID:1080684", "title": "Locomotion in the cat: basic programmes of movement.", "content": "Observations in cats of flexion and extension movements of the 4 limbs have led to the conclusion that the different forms of alternative locomotion (e.g. walking, trotting, swimming) and in-phase locomotion (galloping, jumping) result from the interaction of 'programmes' for the coordination of (1) the homologous limbs (pair of hindlimbs or pair of forelimbs) and (2) the homolateral limbs (hind- and forelimb of the same side of the body). The movements of the homologous pairs of limbs are coupled out of phase in alternate locomotion and approximately in phase in the phase form of locomotion. The movements of the homolateral pairs of limbs occur approximately out of phase in the trotting type of coupling and approximately in phase in the pacing type of coupling. Transitions between the different forms of coupling occur abruptly over 1 or 2 steps. Therefore, for each type of coupling (homologous or homolateral) there are two distinct forms or 'programmes' of movement. The hypothesis is advanced that (a) all the characteristic patterns of locomotion in the cat result from different combinations of these 'programmes' of homologous and homolateral limb coupling; (b) the 'programmes' are mutually self reinforcing in the gaits in which the coordination of the movements of the 4 limbs is bilaterally symmetrical; (c) the 'programmes' act in competition in certain gaits which are not bilaterally symmetrical giving rise at times to a changing gait pattern, and (d) the temporary dominance of one 'programme' or another can determine the gait of the particular step.", "contents": "Locomotion in the cat: basic programmes of movement. Observations in cats of flexion and extension movements of the 4 limbs have led to the conclusion that the different forms of alternative locomotion (e.g. walking, trotting, swimming) and in-phase locomotion (galloping, jumping) result from the interaction of 'programmes' for the coordination of (1) the homologous limbs (pair of hindlimbs or pair of forelimbs) and (2) the homolateral limbs (hind- and forelimb of the same side of the body). The movements of the homologous pairs of limbs are coupled out of phase in alternate locomotion and approximately in phase in the phase form of locomotion. The movements of the homolateral pairs of limbs occur approximately out of phase in the trotting type of coupling and approximately in phase in the pacing type of coupling. Transitions between the different forms of coupling occur abruptly over 1 or 2 steps. Therefore, for each type of coupling (homologous or homolateral) there are two distinct forms or 'programmes' of movement. The hypothesis is advanced that (a) all the characteristic patterns of locomotion in the cat result from different combinations of these 'programmes' of homologous and homolateral limb coupling; (b) the 'programmes' are mutually self reinforcing in the gaits in which the coordination of the movements of the 4 limbs is bilaterally symmetrical; (c) the 'programmes' act in competition in certain gaits which are not bilaterally symmetrical giving rise at times to a changing gait pattern, and (d) the temporary dominance of one 'programme' or another can determine the gait of the particular step."}
{"id": "PMID:1080690", "title": "Different sites of membrane action for tetrodotoxin and lipid-soluble anesthetics.", "content": "1. To examine whether different local anesthetics act on different or similar sites in the nerve membrane, the effects of combinations of lipid-soluble anesthetics and tetrodotoxin (TTX) were studied on the compound action potential of the frog sciatic nerve. 2. The nerve-blocking potency of TTX was potentiated 5-fold by benzyl alcohol and 10-fold by lidocaine, at concentrations of these latter two drugs which were just barely anesthetic; no such synergism occurred between saxitoxin (STX) and TTX, their combined effect being merely additive. 3. Simple additive effects also occurred between benzyl alcohol and phenol, benzyl alcohol and urethane, as well as lidocaine with a spirosuccinimide, RAC 109 II. 4. Synergism occurred between STX and 2-aminobenzimidazole. 5. A small but consistent synergism occurred between lidocaine and benzyl alcohol. 6. It is concluded that uncharged anesthetics, tertiary amines, and TTX act via different membrane binding sites which are all closely associated with the Na+ conductance channels.", "contents": "Different sites of membrane action for tetrodotoxin and lipid-soluble anesthetics. 1. To examine whether different local anesthetics act on different or similar sites in the nerve membrane, the effects of combinations of lipid-soluble anesthetics and tetrodotoxin (TTX) were studied on the compound action potential of the frog sciatic nerve. 2. The nerve-blocking potency of TTX was potentiated 5-fold by benzyl alcohol and 10-fold by lidocaine, at concentrations of these latter two drugs which were just barely anesthetic; no such synergism occurred between saxitoxin (STX) and TTX, their combined effect being merely additive. 3. Simple additive effects also occurred between benzyl alcohol and phenol, benzyl alcohol and urethane, as well as lidocaine with a spirosuccinimide, RAC 109 II. 4. Synergism occurred between STX and 2-aminobenzimidazole. 5. A small but consistent synergism occurred between lidocaine and benzyl alcohol. 6. It is concluded that uncharged anesthetics, tertiary amines, and TTX act via different membrane binding sites which are all closely associated with the Na+ conductance channels."}
{"id": "PMID:1080692", "title": "Evidence for origin of certain childhood acute lymphoblastic leukemias and lymphomas in thymus-derived lymphocytes.", "content": "Lymphoblasts from children with acute lymphoblastic leukemia (ALL) or malignant lymphoblastic lymphoma were studied using surface markers characteristic of T and B lymphocytes. A B-cell marker, i.e. surface immunoglobulin, was absent in all cases studied. Fouteen of 22 children (64%) had lymphoblasts with one or both markers of T lymphocytes, i.e. receptors for sheep erythrocytes (E) and/or human T-lymphocyte antigen (HTLA) detectable using heterologous antithymocyte sera absorbed with B lymphocytes. In all instances, lymphoblasts which carried E receptors also carried HTLA. However, lymphoblasts in 6 cases carried HTLA but not E receptors. It is possible that ALL may often involve T lymphocytes which are early in differentiation (i.e. prior to development of E receptors) or, alternatively, that E receptors may be lost from T cells following malignant transformation. Thymus enlargement was found only in cases of ALL or lymphoma where T markers were present. Lymphoblasts carried the same markers when examined in various sites and at various times from the same patient.", "contents": "Evidence for origin of certain childhood acute lymphoblastic leukemias and lymphomas in thymus-derived lymphocytes. Lymphoblasts from children with acute lymphoblastic leukemia (ALL) or malignant lymphoblastic lymphoma were studied using surface markers characteristic of T and B lymphocytes. A B-cell marker, i.e. surface immunoglobulin, was absent in all cases studied. Fouteen of 22 children (64%) had lymphoblasts with one or both markers of T lymphocytes, i.e. receptors for sheep erythrocytes (E) and/or human T-lymphocyte antigen (HTLA) detectable using heterologous antithymocyte sera absorbed with B lymphocytes. In all instances, lymphoblasts which carried E receptors also carried HTLA. However, lymphoblasts in 6 cases carried HTLA but not E receptors. It is possible that ALL may often involve T lymphocytes which are early in differentiation (i.e. prior to development of E receptors) or, alternatively, that E receptors may be lost from T cells following malignant transformation. Thymus enlargement was found only in cases of ALL or lymphoma where T markers were present. Lymphoblasts carried the same markers when examined in various sites and at various times from the same patient."}
{"id": "PMID:1080693", "title": "Electron microscopic observations on the retino-preoptic pathway of Rana temporaria L.", "content": "Electron microscopic observations confirm the presence of optic terminals of retinal origin in the preoptic area of Rana temporaria. After unilateral section of the optic nerve, degenerating axon terminals were observed among the neurosecretory cells of the preoptic nucleus, both ipsilaterally and contralaterally. The retino-preoptic terminals apparently establish axo-dendritic synapses with non-neurosecretory neurons. Many more degenerating fibres were seen in the ipsilateral preoptic nucleus than in the contralateral nucleus. These are presumed to pass through and to cross in the dorsal posterior part of the optic chiasm.", "contents": "Electron microscopic observations on the retino-preoptic pathway of Rana temporaria L. Electron microscopic observations confirm the presence of optic terminals of retinal origin in the preoptic area of Rana temporaria. After unilateral section of the optic nerve, degenerating axon terminals were observed among the neurosecretory cells of the preoptic nucleus, both ipsilaterally and contralaterally. The retino-preoptic terminals apparently establish axo-dendritic synapses with non-neurosecretory neurons. Many more degenerating fibres were seen in the ipsilateral preoptic nucleus than in the contralateral nucleus. These are presumed to pass through and to cross in the dorsal posterior part of the optic chiasm."}
{"id": "PMID:1080694", "title": "Scanning electron microscopy of the wall of the third ventricle of the brain of Rana temporaria. III. Electron microscopy of the ventricular surface of the median eminence.", "content": "From combined scanning and transmission electron microscopic studies it is concluded (1) that all surface specializations of the median eminence of Rana temporaria are protrusions of ependymal cells; (2) that the microvillus-like structures of the median eminence are transient differentiations of the ependymal cell surface, associated with the activity of the ependymal cells.", "contents": "Scanning electron microscopy of the wall of the third ventricle of the brain of Rana temporaria. III. Electron microscopy of the ventricular surface of the median eminence. From combined scanning and transmission electron microscopic studies it is concluded (1) that all surface specializations of the median eminence of Rana temporaria are protrusions of ependymal cells; (2) that the microvillus-like structures of the median eminence are transient differentiations of the ependymal cell surface, associated with the activity of the ependymal cells."}
{"id": "PMID:1080696", "title": "Determinants of reversible asynergy. The native coronary circulation.", "content": "To evaluate the influence of the native coronary circulation on the reversibility of asynergy, ventriculograms before and after sublingual nitroglycerin were performed in 51 patients with coronary artery disease and asynergy. The severity of stenotic lesions and caliber of the distal coronary vessels were determined by comparison with external catheter tip diameter corrected for magnification. Of 42 asynergic zones associated with larger than or equal to 90% proximal coronary occlusion, 27 (64%) were akinetic or dyskinetic while only 11 of 38 zones (29%) with less than 90% occlusion showed akinesis (P less than 0.005). Twenty-six of the 38 asynergic zones (69%) with less than 90% occlusion were reversible in contrast to 19 of the 42 zones (45%) with larger than or equal to 90% occlusion (P less than 0.05). Coronary collaterals were observed in 23 of 42 (55%) zones with larger than or equal to 90% occlusion in contrast to only 11 of 38 zones (29%) with less than 90% occlusion (P less than 0.05). Of the zones with both greater than or equal to 90% occlusion and collaterals, 74% were reversible, in contrast to only 11% without collaterals (P less than 0.001). Of the asynergic zones without collaterals, 63% with less than 90% occlusion were reversible in contrast to only 11% with larger than or equal to 90% occlusion (P less than 0.001). Pathologic Q waves were associated with 24 of 42 zones (57%) with larger than or equal to 90% occlusion compared to only nine of the 38 zones (24%) with less than 90% occlusion (P less than 0.01). The presence of Q waves was associated with a significant decrease in the incidence of reversibility regardless of the degree of coronary occlusion. Excluding the asynergic zones with either collaterals or Q waves, 79% with less than 90% occlusion were reversible in contrast to only 37% with larger than or equal to 90% coronary occlusion (P less than 0.05). In contrast, the caliber of the distal vessel could not be correlated with either the severity of asynergy or the presence of collaterals and was similar in both reversible and irreversible asynergic zones. In summary, larger than or equal to 90% proximal stenosis is associated with severe asynergy which is less likely to be reversible compared to asynergy associated with less than 90% coronary occlusion. In the presence of larger than or equal to 90% occlusion, coronary collaterals are associated with a significantly higher incidence of reversible asynergy and thus appear to serve a protective function. However, the caliber of the distal vessel per se does not effect the severity or reversibility of asynergy.", "contents": "Determinants of reversible asynergy. The native coronary circulation. To evaluate the influence of the native coronary circulation on the reversibility of asynergy, ventriculograms before and after sublingual nitroglycerin were performed in 51 patients with coronary artery disease and asynergy. The severity of stenotic lesions and caliber of the distal coronary vessels were determined by comparison with external catheter tip diameter corrected for magnification. Of 42 asynergic zones associated with larger than or equal to 90% proximal coronary occlusion, 27 (64%) were akinetic or dyskinetic while only 11 of 38 zones (29%) with less than 90% occlusion showed akinesis (P less than 0.005). Twenty-six of the 38 asynergic zones (69%) with less than 90% occlusion were reversible in contrast to 19 of the 42 zones (45%) with larger than or equal to 90% occlusion (P less than 0.05). Coronary collaterals were observed in 23 of 42 (55%) zones with larger than or equal to 90% occlusion in contrast to only 11 of 38 zones (29%) with less than 90% occlusion (P less than 0.05). Of the zones with both greater than or equal to 90% occlusion and collaterals, 74% were reversible, in contrast to only 11% without collaterals (P less than 0.001). Of the asynergic zones without collaterals, 63% with less than 90% occlusion were reversible in contrast to only 11% with larger than or equal to 90% occlusion (P less than 0.001). Pathologic Q waves were associated with 24 of 42 zones (57%) with larger than or equal to 90% occlusion compared to only nine of the 38 zones (24%) with less than 90% occlusion (P less than 0.01). The presence of Q waves was associated with a significant decrease in the incidence of reversibility regardless of the degree of coronary occlusion. Excluding the asynergic zones with either collaterals or Q waves, 79% with less than 90% occlusion were reversible in contrast to only 37% with larger than or equal to 90% coronary occlusion (P less than 0.05). In contrast, the caliber of the distal vessel could not be correlated with either the severity of asynergy or the presence of collaterals and was similar in both reversible and irreversible asynergic zones. In summary, larger than or equal to 90% proximal stenosis is associated with severe asynergy which is less likely to be reversible compared to asynergy associated with less than 90% coronary occlusion. In the presence of larger than or equal to 90% occlusion, coronary collaterals are associated with a significantly higher incidence of reversible asynergy and thus appear to serve a protective function. However, the caliber of the distal vessel per se does not effect the severity or reversibility of asynergy."}
{"id": "PMID:1080700", "title": "Transbronchial lung biopsy via the fiberoptic bronchoscope. Experience with 107 consecutive cases and comparison with bronchial brushing.", "content": "Transbronchial lung biopsy (TBB) was performed during fiberoptic bronchoscopy under fluoroscopic guidance in 107 patients. TBB was diagnostic in 17 of 21 (81 percent) cases with localized malignant lesions greater than 4 cm in diameter and bronchial brushing was positive in 12 (57 percent). TBB was diagnostic in 14 of 24 (58 percent) cases with localized malignant lesions equal to or less than 4 cm in diameter, whereas brush biopsy was positive in 7 (29 percent). In 13 cases with suspected Pneumocystis carinii pneumonia, the combination of TBB and brush biopsy was diagnostic of P carinii pneumonia in all 11 patients; TBB was diagnostic in 10 and brush biopsy diagnostic in 5. Cytomegalovirus pneumonitis was diagnosed in the other two cases. In 20 patients with localized infiltrates or nodules, a TBB diagnosis of acute or chronic inflammation excluded malignancy in 15 of 18 cases (follow-up 3 to 24 months) and tuberculosis was diagnosed in 2. Malignancy was found in one patient with acute inflammation on TBB. TBB accurately diagnosed 23 of 29 (79 percent) cases of diffuse lung disease. Following TBB, one patient had pneumothorax and nine patients had hemoptysis not requiring treatment. TBB and bronchial brushing via the flexible fiberoptic bronchoscope offer good diagnostic accuracy and a low complication rate in both diffuse and localized lung diseases.", "contents": "Transbronchial lung biopsy via the fiberoptic bronchoscope. Experience with 107 consecutive cases and comparison with bronchial brushing. Transbronchial lung biopsy (TBB) was performed during fiberoptic bronchoscopy under fluoroscopic guidance in 107 patients. TBB was diagnostic in 17 of 21 (81 percent) cases with localized malignant lesions greater than 4 cm in diameter and bronchial brushing was positive in 12 (57 percent). TBB was diagnostic in 14 of 24 (58 percent) cases with localized malignant lesions equal to or less than 4 cm in diameter, whereas brush biopsy was positive in 7 (29 percent). In 13 cases with suspected Pneumocystis carinii pneumonia, the combination of TBB and brush biopsy was diagnostic of P carinii pneumonia in all 11 patients; TBB was diagnostic in 10 and brush biopsy diagnostic in 5. Cytomegalovirus pneumonitis was diagnosed in the other two cases. In 20 patients with localized infiltrates or nodules, a TBB diagnosis of acute or chronic inflammation excluded malignancy in 15 of 18 cases (follow-up 3 to 24 months) and tuberculosis was diagnosed in 2. Malignancy was found in one patient with acute inflammation on TBB. TBB accurately diagnosed 23 of 29 (79 percent) cases of diffuse lung disease. Following TBB, one patient had pneumothorax and nine patients had hemoptysis not requiring treatment. TBB and bronchial brushing via the flexible fiberoptic bronchoscope offer good diagnostic accuracy and a low complication rate in both diffuse and localized lung diseases."}
{"id": "PMID:1080701", "title": "Spontaneous graft closure in anomalous origin of the left coronary artery.", "content": "Several reports of successful correction of anomalous origin of the left coronary artery from the pulmonary artery utilizing a graft to the ascending aorta have demonstrated the feasibility of this procedure. The patient described in this report developed proved delayed occlusion of the saphenous vein graft with a fatal outcome. This was a result of intimal fibrous hyperplasia identical to that seen in adults following the placement of the saphenous vein bypass graft for treatment of atherosclerotic coronary disease.", "contents": "Spontaneous graft closure in anomalous origin of the left coronary artery. Several reports of successful correction of anomalous origin of the left coronary artery from the pulmonary artery utilizing a graft to the ascending aorta have demonstrated the feasibility of this procedure. The patient described in this report developed proved delayed occlusion of the saphenous vein graft with a fatal outcome. This was a result of intimal fibrous hyperplasia identical to that seen in adults following the placement of the saphenous vein bypass graft for treatment of atherosclerotic coronary disease."}
{"id": "PMID:1080704", "title": "[Clinical and prognostic significance of antinuclear factors during D-penicillamine treatment (author's transl)].", "content": "Antinuclear factors were demonstrated during D-penicillamine treatment in patients with rheumatiod arthritis (72) and scleroderma (7). While in 13 antinuclear factors were demonstrable already before treatment was started, they occurred in the course of treatment in another 34. D-penicillamine administration had to be discontinued in 18 because of severe side effects, with a marked correlation between  their severity and the presence of antinuclear factors. Side effects requiring stoppage of the drug were especially common in patients who had antinuclear factors even before onset of treatment. The severest complication of D-penincillamine treatment, renal damage, was found only in patients with antinuclear factors, in this instance antibodies against denatured DNA. The following therapeutic consequences should be drawn from these findings: (1) D-penicillamine should, if possible, not be given to any patient with antinuclear factors; (2) in patients in whom antinuclear factors occur in the course of D-penicillamine treatment but its administration is to be continued, blood picture, renal functions and urine must be regularly tested.", "contents": "[Clinical and prognostic significance of antinuclear factors during D-penicillamine treatment (author's transl)]. Antinuclear factors were demonstrated during D-penicillamine treatment in patients with rheumatiod arthritis (72) and scleroderma (7). While in 13 antinuclear factors were demonstrable already before treatment was started, they occurred in the course of treatment in another 34. D-penicillamine administration had to be discontinued in 18 because of severe side effects, with a marked correlation between  their severity and the presence of antinuclear factors. Side effects requiring stoppage of the drug were especially common in patients who had antinuclear factors even before onset of treatment. The severest complication of D-penincillamine treatment, renal damage, was found only in patients with antinuclear factors, in this instance antibodies against denatured DNA. The following therapeutic consequences should be drawn from these findings: (1) D-penicillamine should, if possible, not be given to any patient with antinuclear factors; (2) in patients in whom antinuclear factors occur in the course of D-penicillamine treatment but its administration is to be continued, blood picture, renal functions and urine must be regularly tested."}
{"id": "PMID:1080705", "title": "[Clinical course of typhoid in the Heidelberg region (author's transl)].", "content": "In the course of a typhoid epidemic during the autumn of 1974 in the Heidelberg region 74 persons were treated in hospital. Chloramphenicol was give to 45, ampicillin to 19. The former, in daily doses of 2.0 g, gave worse results if given for only two instead of three weeks. In comparison, ampicillin was less effective. A second course of treatment became necessary in 13 patients, with trimethoprim-sulphamethoxazole (Bactrim) being succesful in all, although the follow-up period is still too short for definitive results. Three complications occurred: one case of massive bleeding from the gut requiring operation and followed some weeks later by a HBS-antigen-negative hepatitis; one case of typhoma (several weeks after the end of antibiotic treatment), requiring operative removal; a case of febrile abortion in the second month of pregnancy.", "contents": "[Clinical course of typhoid in the Heidelberg region (author's transl)]. In the course of a typhoid epidemic during the autumn of 1974 in the Heidelberg region 74 persons were treated in hospital. Chloramphenicol was give to 45, ampicillin to 19. The former, in daily doses of 2.0 g, gave worse results if given for only two instead of three weeks. In comparison, ampicillin was less effective. A second course of treatment became necessary in 13 patients, with trimethoprim-sulphamethoxazole (Bactrim) being succesful in all, although the follow-up period is still too short for definitive results. Three complications occurred: one case of massive bleeding from the gut requiring operation and followed some weeks later by a HBS-antigen-negative hepatitis; one case of typhoma (several weeks after the end of antibiotic treatment), requiring operative removal; a case of febrile abortion in the second month of pregnancy."}
{"id": "PMID:1080707", "title": "The amino-acid sequence of the most acidic major parvalbumin from frog muscle.", "content": "The primary structure of the most acidic (pI - 4.50) of the two major parvalbumins from frog (Rana esculenta) has been determined from a study of its trypsic peptides and of overlapping peptides generated by limited trypsic digestion, chymotrypsic digestion and N-bromosuccinimide cleavage of the protein. The amino acid sequence so obtained is considered in comparison with those known for other parvalbumins and for rabbit troponin-C.", "contents": "The amino-acid sequence of the most acidic major parvalbumin from frog muscle. The primary structure of the most acidic (pI - 4.50) of the two major parvalbumins from frog (Rana esculenta) has been determined from a study of its trypsic peptides and of overlapping peptides generated by limited trypsic digestion, chymotrypsic digestion and N-bromosuccinimide cleavage of the protein. The amino acid sequence so obtained is considered in comparison with those known for other parvalbumins and for rabbit troponin-C."}
{"id": "PMID:1080708", "title": "Dilantin-calcium interaction and active Na transport in frog skin.", "content": "The effects of diphenylhydantoin (DPH) and calcium were simultaneously studied on solute transport in frog skin. In the presence of external Ca, DPH elicited a significant increase in active Na transport; in its absence, DPH had no significant effect on active transport. DPH increased passive permeability, but this action was independent of Ca. It appears that the increase in active transport elicited by DPH is due to a specific interaction with Ca at the transport site.", "contents": "Dilantin-calcium interaction and active Na transport in frog skin. The effects of diphenylhydantoin (DPH) and calcium were simultaneously studied on solute transport in frog skin. In the presence of external Ca, DPH elicited a significant increase in active Na transport; in its absence, DPH had no significant effect on active transport. DPH increased passive permeability, but this action was independent of Ca. It appears that the increase in active transport elicited by DPH is due to a specific interaction with Ca at the transport site."}
{"id": "PMID:1080709", "title": "Cell membranes as sources of granulocyte colony stimulating activities.", "content": "Subcellular localization of factors associated with stimulation of granulocyte progenitors (CFU-c) to form colonies in vitro was investigated. Human peripheral blood leukocytes were fractionated into three subcellular fractions. After solubilization with sodium dodecyl sulfate, fractions were assessed for ability to stimulate colony formation by CFU-c. The fraction containing cell membranes proved to be the richest source of stimulating factors. Apparent molecular weights for these factors as determined by gel filtration were equivalent to those obtained for factors from purified peripheral leukocyte conditioned medium. The results suggest that at least some granulocyte colony-stimulating factors should be regarded as membrane-active agents.", "contents": "Cell membranes as sources of granulocyte colony stimulating activities. Subcellular localization of factors associated with stimulation of granulocyte progenitors (CFU-c) to form colonies in vitro was investigated. Human peripheral blood leukocytes were fractionated into three subcellular fractions. After solubilization with sodium dodecyl sulfate, fractions were assessed for ability to stimulate colony formation by CFU-c. The fraction containing cell membranes proved to be the richest source of stimulating factors. Apparent molecular weights for these factors as determined by gel filtration were equivalent to those obtained for factors from purified peripheral leukocyte conditioned medium. The results suggest that at least some granulocyte colony-stimulating factors should be regarded as membrane-active agents."}
{"id": "PMID:1080710", "title": "Serum potentiation of granulocyte and macrophage colony formation in vitro.", "content": "Addition of serum or plasma from many species increased the number and size of neutrophilic granulocyte and macrophage colonies formed in vitro by mouse bone marrow cells after stimulation by a variety of preparations containing colony stimulating factor (CSF). The active factor in serum was heat-labile, non-dialyzable and eluted from G-100 columns in the serum albumin region. Serum non-selectively affected all cells potentially capable of forming granulocytic and macrophage colonies but did not potentiate the formation of eosinophilic or megakaryocytic colonies. The potentiating activity of mouse serum was increased by prior injection of the mice with endotoxin but was not affected by prior whole-body irradiation. Colony potentiating activity in plasma from patients with myeloid of lymphoid leukemia did not differ from that of normal human plasma.", "contents": "Serum potentiation of granulocyte and macrophage colony formation in vitro. Addition of serum or plasma from many species increased the number and size of neutrophilic granulocyte and macrophage colonies formed in vitro by mouse bone marrow cells after stimulation by a variety of preparations containing colony stimulating factor (CSF). The active factor in serum was heat-labile, non-dialyzable and eluted from G-100 columns in the serum albumin region. Serum non-selectively affected all cells potentially capable of forming granulocytic and macrophage colonies but did not potentiate the formation of eosinophilic or megakaryocytic colonies. The potentiating activity of mouse serum was increased by prior injection of the mice with endotoxin but was not affected by prior whole-body irradiation. Colony potentiating activity in plasma from patients with myeloid of lymphoid leukemia did not differ from that of normal human plasma."}
{"id": "PMID:1080711", "title": "The influence of mouse sera on colony formation and on the production of colony stimulating factor in vitro.", "content": "Results of studies on the in vitro production and release of colony stimulating factor (CSF), by bone associated cells cultivated in the presence or absence of mouse sera, are presented. Tissues from three strains of mice were shown to be equally effective in the production of CSF in vitro. Priming of the tissue donors by endotoxin 4 hours previously, or by irradiation 1, 7 or 15 days previously, significantly increased the CSF production. The addition of serum from normal mice to the culture medium had no effect on CSF production by tissue from normal mice but regularly inhibited CSF production by bones from irradiated animals. The inhibition was not due to the toxic effect of the sera on the CSF producing cells and was more pronounced in the cultures of NMRI than in the C57BL tissues. Some inhibition was also observed in cultures of tissue from endotoxin-primed NMRI mice. Extraction of the sera with ether removed the inhibitory material. The sera which inhibited the CSF production in vitro also inhibited the growth of granulocytic and macrophage colonies in soft agar cultures but did not inhibit the growth and DNA synthesis of cultured L-cells.", "contents": "The influence of mouse sera on colony formation and on the production of colony stimulating factor in vitro. Results of studies on the in vitro production and release of colony stimulating factor (CSF), by bone associated cells cultivated in the presence or absence of mouse sera, are presented. Tissues from three strains of mice were shown to be equally effective in the production of CSF in vitro. Priming of the tissue donors by endotoxin 4 hours previously, or by irradiation 1, 7 or 15 days previously, significantly increased the CSF production. The addition of serum from normal mice to the culture medium had no effect on CSF production by tissue from normal mice but regularly inhibited CSF production by bones from irradiated animals. The inhibition was not due to the toxic effect of the sera on the CSF producing cells and was more pronounced in the cultures of NMRI than in the C57BL tissues. Some inhibition was also observed in cultures of tissue from endotoxin-primed NMRI mice. Extraction of the sera with ether removed the inhibitory material. The sera which inhibited the CSF production in vitro also inhibited the growth of granulocytic and macrophage colonies in soft agar cultures but did not inhibit the growth and DNA synthesis of cultured L-cells."}
{"id": "PMID:1080712", "title": "Enhancement of chicken hemagglutinin response by thymocytes.", "content": "Antigen responsive cells, detectable with the cell transfer antibody assay, are abundant in the blood and spleen of chickens after immunization. In experiments where serial dilutions of donor immunocyte-antigen mixture were cultured in host embryos, each two-fold dilution of the mixture resulted in approximately a four-fold reduction in hemagglutinin output. When higher than normal concentrations of donor buffy coat were used, however, serial dilutions resulted in antibody output that was more directly proportional. The overall dose-response curve of relative donor cell number to antibody production appears to be biphasic, with a slope of a first order reaction at higher concentrations, and a steeper slope at lower concentrations of donor cells. The hemagglutinin response in the adoptive cell transfer system was enhanced when autologous thymocytes were added to the immunocyte-antigen mixture. Data from the kinetic and thymocyte supplement experiments taken together strongly indicate that the mouse erythrocyte antigen responsive cells of the chicken, like their mammalian counterpart the antigen-reactive units, involve the cooperation of T and B type lymphocytes.", "contents": "Enhancement of chicken hemagglutinin response by thymocytes. Antigen responsive cells, detectable with the cell transfer antibody assay, are abundant in the blood and spleen of chickens after immunization. In experiments where serial dilutions of donor immunocyte-antigen mixture were cultured in host embryos, each two-fold dilution of the mixture resulted in approximately a four-fold reduction in hemagglutinin output. When higher than normal concentrations of donor buffy coat were used, however, serial dilutions resulted in antibody output that was more directly proportional. The overall dose-response curve of relative donor cell number to antibody production appears to be biphasic, with a slope of a first order reaction at higher concentrations, and a steeper slope at lower concentrations of donor cells. The hemagglutinin response in the adoptive cell transfer system was enhanced when autologous thymocytes were added to the immunocyte-antigen mixture. Data from the kinetic and thymocyte supplement experiments taken together strongly indicate that the mouse erythrocyte antigen responsive cells of the chicken, like their mammalian counterpart the antigen-reactive units, involve the cooperation of T and B type lymphocytes."}
{"id": "PMID:1080715", "title": "Relationships between H-2 Region and surface characteristics of mouse spleen cells.", "content": "The purpose of this investigation was to study whether allelic variations at the H-2 region which induce differences in the peptide composition of some glycoproteins of cell surface, also induce variations in electrical charges at the cell membrane. The surface charge was assessed by the electrophoretic mobility of the cell. Tests with different congenic strains were undertaken on spleen cells which were assumed to contain two major lymphocyte populations with different surface charge. The data show that the mean electrophoretic mobility of the low mobility lymphocytes was independent of the strain. By contrast, some H-2 haplotypes give 8% difference in surface charge of high mobility lymphocytes. These results suggest that the H-2 region plays a role in the regulation of ionized groups of cell surface in some but not all populations of spleen lymphocytes. The high and low mobility lymphocyte populations are assumed to represent T and B lymphocytes, respectively. The proportions of T lymphocytes obtained with electrophoretic marker were higher than those observed in other strains with currently available markers. It is suggested that the high mobility population contains T and \"null\" lymphocytes. Moreover, the data show that the H-2 region controls the relative proportions of T (or T and \"null\") and B lymphocytes, the H-2s specificity inducing a B/T ratio different from that induced by other specificities.", "contents": "Relationships between H-2 Region and surface characteristics of mouse spleen cells. The purpose of this investigation was to study whether allelic variations at the H-2 region which induce differences in the peptide composition of some glycoproteins of cell surface, also induce variations in electrical charges at the cell membrane. The surface charge was assessed by the electrophoretic mobility of the cell. Tests with different congenic strains were undertaken on spleen cells which were assumed to contain two major lymphocyte populations with different surface charge. The data show that the mean electrophoretic mobility of the low mobility lymphocytes was independent of the strain. By contrast, some H-2 haplotypes give 8% difference in surface charge of high mobility lymphocytes. These results suggest that the H-2 region plays a role in the regulation of ionized groups of cell surface in some but not all populations of spleen lymphocytes. The high and low mobility lymphocyte populations are assumed to represent T and B lymphocytes, respectively. The proportions of T lymphocytes obtained with electrophoretic marker were higher than those observed in other strains with currently available markers. It is suggested that the high mobility population contains T and \"null\" lymphocytes. Moreover, the data show that the H-2 region controls the relative proportions of T (or T and \"null\") and B lymphocytes, the H-2s specificity inducing a B/T ratio different from that induced by other specificities."}
{"id": "PMID:1080717", "title": "Haemochromatosis, active chronic hepatitis, and familial IgA deficiency.", "content": "A man aged 38 years developed chronic liver disease with characteristics of both haemochromatosis and active chronic hepatitis. Two of his relatives were found to have selective IgA deficiency. It is suggested that this case provides evidence that haemochromatosis may initiate active chronic hepatitis in a genetically susceptible subject.", "contents": "Haemochromatosis, active chronic hepatitis, and familial IgA deficiency. A man aged 38 years developed chronic liver disease with characteristics of both haemochromatosis and active chronic hepatitis. Two of his relatives were found to have selective IgA deficiency. It is suggested that this case provides evidence that haemochromatosis may initiate active chronic hepatitis in a genetically susceptible subject."}
{"id": "PMID:1080718", "title": "Prevention by glucagon of experimental stress induced hemorrhagic gastritis.", "content": "Glucagon was effective in decreasing acute gastric mucosal lesions, blood loss and gastric acid secretion in the cold restraint stressed rat.", "contents": "Prevention by glucagon of experimental stress induced hemorrhagic gastritis. Glucagon was effective in decreasing acute gastric mucosal lesions, blood loss and gastric acid secretion in the cold restraint stressed rat."}
{"id": "PMID:1080740", "title": "A histochemical study on the central effect of monoamine precursors.", "content": "A histochemical investigation has been made on the central monoamine neurons after the administration of monoamine precursors and peripheral decarboxylase inhibitor in experimental animals and the results are summarized as below: (1) Catecholamine fluorescence in the brain reduces and yellowish indoleamine fluorescence manifests after the treatment with 5HTP alone and Ro4-4602 plus 5HTP. These findings support the view that catecholamine is likely to be replaced by indoleamine. (2) Ro4-4602 does not cause directly any change in central catecholamine neurons except in median eminence where the inhibitor causes a decrease in dopamine fluorescence. The findings indicate that the inhibitor may act in the hypothalamus as same manner as in the peripheries, because the hypothalamus is presumably lacking of blood brain barrier. (3) L-Dopa alone and L-Dopa plus Ro4-4602 enhance dopamine fluorescence in the substantia nigra and the median eminence, especially an increase in the extra-neuronal fluorescence can be seen in the latter. This indicates the releasing of amines from cell body into extraneuronal spaces.", "contents": "A histochemical study on the central effect of monoamine precursors. A histochemical investigation has been made on the central monoamine neurons after the administration of monoamine precursors and peripheral decarboxylase inhibitor in experimental animals and the results are summarized as below: (1) Catecholamine fluorescence in the brain reduces and yellowish indoleamine fluorescence manifests after the treatment with 5HTP alone and Ro4-4602 plus 5HTP. These findings support the view that catecholamine is likely to be replaced by indoleamine. (2) Ro4-4602 does not cause directly any change in central catecholamine neurons except in median eminence where the inhibitor causes a decrease in dopamine fluorescence. The findings indicate that the inhibitor may act in the hypothalamus as same manner as in the peripheries, because the hypothalamus is presumably lacking of blood brain barrier. (3) L-Dopa alone and L-Dopa plus Ro4-4602 enhance dopamine fluorescence in the substantia nigra and the median eminence, especially an increase in the extra-neuronal fluorescence can be seen in the latter. This indicates the releasing of amines from cell body into extraneuronal spaces."}
{"id": "PMID:1080741", "title": "Immunosuppressor T cells in tumor bearing host.", "content": "Thymus or spleen cells of tumor bearing animals (TBA) (methylcholanthrene induced sarcoma bearing A/Jax mice) were shown to possess immunosuppressor cells regulating the immune response to the tumor in syngeneic animals. The immunosuppressive activity of these cells of TBA was totally abolished by the in vitro treatment of anti-theta serum and complement. Soluble factor(s) with identical suppressive activity was isolated from the thymus cells of TBA and its molecular size was estimated to be lower than that of serum albumin in terms of its elution behaviour on gel filtration through a Sephadex G-200 column. These immunosuppressor T cells or their soluble factor(s) may be largely responsible for the growth of antigenic tumors.", "contents": "Immunosuppressor T cells in tumor bearing host. Thymus or spleen cells of tumor bearing animals (TBA) (methylcholanthrene induced sarcoma bearing A/Jax mice) were shown to possess immunosuppressor cells regulating the immune response to the tumor in syngeneic animals. The immunosuppressive activity of these cells of TBA was totally abolished by the in vitro treatment of anti-theta serum and complement. Soluble factor(s) with identical suppressive activity was isolated from the thymus cells of TBA and its molecular size was estimated to be lower than that of serum albumin in terms of its elution behaviour on gel filtration through a Sephadex G-200 column. These immunosuppressor T cells or their soluble factor(s) may be largely responsible for the growth of antigenic tumors."}
{"id": "PMID:1080742", "title": "In Vitro studies on human B and T cell purified populations. Stimulation by mitogens and allogeneic cells, and quantitative binding of phytomitogens.", "content": "The property of T cells to form rosettes with sheep red blood cells has been used to separate peripheral blood lymphocytes into purified T- and B-cell suspensions after density gradient centrifugation. A study of lymphocyte markers has shown that 2-6 per cent of E rosettes only were recovered in the B cell-enriched population. Lymphocyte stimulation in vitro was obtained with PHA, con A and PWM in unseparated and T-cell populations, but never in B-cell suspensions. Experiments of recombination between the two purified fractions have demonstrated that 10% of T cells added to B cells were able to induce a response to PHA and PWM. Otherwise, only T cells responded to allogenic stimulation. Lastly, B and T cells seemed to bind qualitatively and quantitatively the same mitogens on their membranes.", "contents": "In Vitro studies on human B and T cell purified populations. Stimulation by mitogens and allogeneic cells, and quantitative binding of phytomitogens. The property of T cells to form rosettes with sheep red blood cells has been used to separate peripheral blood lymphocytes into purified T- and B-cell suspensions after density gradient centrifugation. A study of lymphocyte markers has shown that 2-6 per cent of E rosettes only were recovered in the B cell-enriched population. Lymphocyte stimulation in vitro was obtained with PHA, con A and PWM in unseparated and T-cell populations, but never in B-cell suspensions. Experiments of recombination between the two purified fractions have demonstrated that 10% of T cells added to B cells were able to induce a response to PHA and PWM. Otherwise, only T cells responded to allogenic stimulation. Lastly, B and T cells seemed to bind qualitatively and quantitatively the same mitogens on their membranes."}
{"id": "PMID:1080743", "title": "The activity of T and B lymphocytes in immunity and tolerance to the lymphocytic choriomeningitis virus in mice.", "content": "Treatment with anti-theta serum and the Wigzell column technique for cell separation was employed to study the separate functions of the B and T lymphocytes in the late states of immunity to the LCM virus in mice. The cell preparations examined were mixtures of spleen and lymph node cells from immune mice. The results revealed that the anti-viral effect of such cells after transfer to virus carriers was unimpaired in T cell-enriched and B cell-deprived cell preparations. The anti-viral effect was also retained in cell preparations deprived so much of B cells that no antibody was produced in the virus carrier mice receiving transplants of these cells. The results strongly indicate that the anti-viral effect of late immune cells is not only T cell-dependent but that it is also mediated solely by T cells and, moreover, that antibodies have no or very little influence on the virus elimination. The observation that antibody production could be caused neither by column-passed cells nor by anti-theta serum-treated cells, but was obtained by mixtures of these cells, demonstrates that co-operation between T and B cells is crucial for the LCM antibody response. Accordingly, the convincing demonstration of the absence in the persistent virus carriers of cells which, in respect of antibody production, are able to co-operate either with column-passed or with anti-theta serum-treated immune cells, implies that such animals are extremely deficient as regards immune function of both B and T LCM-primed lymphocytes.", "contents": "The activity of T and B lymphocytes in immunity and tolerance to the lymphocytic choriomeningitis virus in mice. Treatment with anti-theta serum and the Wigzell column technique for cell separation was employed to study the separate functions of the B and T lymphocytes in the late states of immunity to the LCM virus in mice. The cell preparations examined were mixtures of spleen and lymph node cells from immune mice. The results revealed that the anti-viral effect of such cells after transfer to virus carriers was unimpaired in T cell-enriched and B cell-deprived cell preparations. The anti-viral effect was also retained in cell preparations deprived so much of B cells that no antibody was produced in the virus carrier mice receiving transplants of these cells. The results strongly indicate that the anti-viral effect of late immune cells is not only T cell-dependent but that it is also mediated solely by T cells and, moreover, that antibodies have no or very little influence on the virus elimination. The observation that antibody production could be caused neither by column-passed cells nor by anti-theta serum-treated cells, but was obtained by mixtures of these cells, demonstrates that co-operation between T and B cells is crucial for the LCM antibody response. Accordingly, the convincing demonstration of the absence in the persistent virus carriers of cells which, in respect of antibody production, are able to co-operate either with column-passed or with anti-theta serum-treated immune cells, implies that such animals are extremely deficient as regards immune function of both B and T LCM-primed lymphocytes."}
{"id": "PMID:1080744", "title": "Epicutaneous induction of hyporeactivity in contact sensitization. Demonstration of suppressor cells induced by contact with 2,4-dinitrothiocyanatebenzene.", "content": "Partial unresponsiveness to 2,4-dinitrofluorobenzene (DNFB) was produced by the epicutaneous application of 2 per cent 2,4-dinitrothiocyanatebenzene (DNTB) to the dorsum of the ear, 14 and 7 days before sensitization. This state of partial unresponsiveness could be broken by treatment with 300 mg/kg cyclophosphamide (CY) 3 days before the sensitization dose of DNFB. There was a significant increase in the uptake of 125I-labelled iododeoxyuridine in the draining lymph node, 6 days after the application of 2 per cent DNTB and in the contralateral nodes at 4 and 6 days. The state of partial unresponsiveness to DNFB was not associated with a decrease in T-cell proliferation in the draining lymph nodes. The generation of suppressor cells, capable of reacting in the periphery, was demonstrated by passive cell transfer into recipients sensitized to DNFB and depleted of normally produced suppressor cells by pretreatment with CY. These cells were found in spleen and peritoneal exudates of animals treated by the epicutaneous application of 2 per cent DNTB 14 and 7 days previously. It is suggested that the suppressor cells induced by contact with DNTB act by competition with effector cells at the periphery.", "contents": "Epicutaneous induction of hyporeactivity in contact sensitization. Demonstration of suppressor cells induced by contact with 2,4-dinitrothiocyanatebenzene. Partial unresponsiveness to 2,4-dinitrofluorobenzene (DNFB) was produced by the epicutaneous application of 2 per cent 2,4-dinitrothiocyanatebenzene (DNTB) to the dorsum of the ear, 14 and 7 days before sensitization. This state of partial unresponsiveness could be broken by treatment with 300 mg/kg cyclophosphamide (CY) 3 days before the sensitization dose of DNFB. There was a significant increase in the uptake of 125I-labelled iododeoxyuridine in the draining lymph node, 6 days after the application of 2 per cent DNTB and in the contralateral nodes at 4 and 6 days. The state of partial unresponsiveness to DNFB was not associated with a decrease in T-cell proliferation in the draining lymph nodes. The generation of suppressor cells, capable of reacting in the periphery, was demonstrated by passive cell transfer into recipients sensitized to DNFB and depleted of normally produced suppressor cells by pretreatment with CY. These cells were found in spleen and peritoneal exudates of animals treated by the epicutaneous application of 2 per cent DNTB 14 and 7 days previously. It is suggested that the suppressor cells induced by contact with DNTB act by competition with effector cells at the periphery."}
{"id": "PMID:1080745", "title": "Investigation of NZB mice born to BALB/c immunized against NZB allotype.", "content": "Allotype suppression is held to be an example of T-cell suppressor function. Failure of generalized suppressor T-cell function is considered to be responsible for the chronic and progressive disease of the NZB. The possible association of allotype suppression and autoimmune disease has been investigated here in a group of NZB mice, transplanted and born from BALB/c recipients whilst producing anti-NZB allotype. In this situation it was anticipated that if allotype suppression should fail this would coincide with the development of autoimmune disease; both processes reflecting failure of T-cell suppressor function. However, in spite of the fact that allotype suppression was not achieved, certain observations seem important. The fact that NZB born the BALB/c were in no way different from normally derived NZB confirms that the cause of the NZB disease is established prior to the stage of implantation. Maternal influence at or beyond this stage appears of little consequence in terms of effecting the development or progression of the NZB disease. The failure to induce allotype suppression in the NZB is important. In this context this strain appears to be no different from the majority of other strains that have been tested as homozygotes. The suppression obtained with (BALB/c X SJL/J)F1 hybrids in which chronic suppression has been achieved therefore seems to represent an exceptional situation and this suggests that it is perhaps unwise to base general assumptions as to the universal adaptability of T-cell control mechanisms upon unusual findings. It must be remembered that only homozygotes were examined here. Earlier attempts to induce allotype suppression in homozygotes also failed, even in the SJL/J. Since allotype suppression has only been demonstrated in heterozygote mice there is a distinct possibility that this phenomenon never occurs in the homozygote situation. The significance of this possibility is discussed.", "contents": "Investigation of NZB mice born to BALB/c immunized against NZB allotype. Allotype suppression is held to be an example of T-cell suppressor function. Failure of generalized suppressor T-cell function is considered to be responsible for the chronic and progressive disease of the NZB. The possible association of allotype suppression and autoimmune disease has been investigated here in a group of NZB mice, transplanted and born from BALB/c recipients whilst producing anti-NZB allotype. In this situation it was anticipated that if allotype suppression should fail this would coincide with the development of autoimmune disease; both processes reflecting failure of T-cell suppressor function. However, in spite of the fact that allotype suppression was not achieved, certain observations seem important. The fact that NZB born the BALB/c were in no way different from normally derived NZB confirms that the cause of the NZB disease is established prior to the stage of implantation. Maternal influence at or beyond this stage appears of little consequence in terms of effecting the development or progression of the NZB disease. The failure to induce allotype suppression in the NZB is important. In this context this strain appears to be no different from the majority of other strains that have been tested as homozygotes. The suppression obtained with (BALB/c X SJL/J)F1 hybrids in which chronic suppression has been achieved therefore seems to represent an exceptional situation and this suggests that it is perhaps unwise to base general assumptions as to the universal adaptability of T-cell control mechanisms upon unusual findings. It must be remembered that only homozygotes were examined here. Earlier attempts to induce allotype suppression in homozygotes also failed, even in the SJL/J. Since allotype suppression has only been demonstrated in heterozygote mice there is a distinct possibility that this phenomenon never occurs in the homozygote situation. The significance of this possibility is discussed."}
{"id": "PMID:1080746", "title": "Presence and kinetics of sensitized cells in different tissue compartments.", "content": "The question was studied as to whether lymph nodes (LN), spleen (SP) and bone marrow (BM) contain different amounts of sensitized cells at various times after one or repeated immunizations. The following model was used: C57 mice were injected i.p. once, twice or three times with C3H cells. Between 3 and 20 days after the last immunization, LN, SP and BM cell suspensions were tested in vivo for their local cutaneous lymphocyte transfer reactivity in C3H mice and in vitro for their lymphocytotoxicity (partially after preincubation with anti-theta serum). In both test systems similar observations were made: after one sensitization, reactive cells were demonstrated first in the LN and then in the SP, but only during the next few days; BM cell reactivity appeared later. In the first few days after two sensitizations SP cells reacted strongly whereas LN and BM cells reacted weakly; later BM cell reactivity increased while the others decreased. After three sensitizations reactivity of the SP cells predominated during the entire period of observations; the BM cells showed less, the LN cells no reactivity. These findings suggest dependance of T cell-mediated reactivity on (1) the origin of the cells from LN, SP or BM, (2) the time of the harvesting from these tissues, and (3) the degree of sensitization.", "contents": "Presence and kinetics of sensitized cells in different tissue compartments. The question was studied as to whether lymph nodes (LN), spleen (SP) and bone marrow (BM) contain different amounts of sensitized cells at various times after one or repeated immunizations. The following model was used: C57 mice were injected i.p. once, twice or three times with C3H cells. Between 3 and 20 days after the last immunization, LN, SP and BM cell suspensions were tested in vivo for their local cutaneous lymphocyte transfer reactivity in C3H mice and in vitro for their lymphocytotoxicity (partially after preincubation with anti-theta serum). In both test systems similar observations were made: after one sensitization, reactive cells were demonstrated first in the LN and then in the SP, but only during the next few days; BM cell reactivity appeared later. In the first few days after two sensitizations SP cells reacted strongly whereas LN and BM cells reacted weakly; later BM cell reactivity increased while the others decreased. After three sensitizations reactivity of the SP cells predominated during the entire period of observations; the BM cells showed less, the LN cells no reactivity. These findings suggest dependance of T cell-mediated reactivity on (1) the origin of the cells from LN, SP or BM, (2) the time of the harvesting from these tissues, and (3) the degree of sensitization."}
{"id": "PMID:1080747", "title": "Human tumor--lymphocyte interaction in vitro: blastogenesis correlated to detectable immunoglobulin in the biopsy.", "content": "Binding of radioiodinated anti-immunoglobulin (Ig) reagent assayed by acid elutable radioactivity was shown in 18 of 44 cell suspensions (41%) prepared from surgical specimens of human tumors. Aliquots of these biopsies were admixed to autologous lymphocytes and in 13 cases they induced stimulation of DNA synthesis. In only one of these 13 cases was the anti-Ig reagent bound, while among the 26 biopsies with low or no binding capacity 12 (46%) were stimulatory, indicating that immunoglobulin-containing biopsies are not stimulatory. Experiments on 32 lymphocyte preparations from different lymphoid organs suggest that the immunoglobulin detected in the tumor-cell suspension is not derived from the infiltrating lymphoid cells.", "contents": "Human tumor--lymphocyte interaction in vitro: blastogenesis correlated to detectable immunoglobulin in the biopsy. Binding of radioiodinated anti-immunoglobulin (Ig) reagent assayed by acid elutable radioactivity was shown in 18 of 44 cell suspensions (41%) prepared from surgical specimens of human tumors. Aliquots of these biopsies were admixed to autologous lymphocytes and in 13 cases they induced stimulation of DNA synthesis. In only one of these 13 cases was the anti-Ig reagent bound, while among the 26 biopsies with low or no binding capacity 12 (46%) were stimulatory, indicating that immunoglobulin-containing biopsies are not stimulatory. Experiments on 32 lymphocyte preparations from different lymphoid organs suggest that the immunoglobulin detected in the tumor-cell suspension is not derived from the infiltrating lymphoid cells."}
{"id": "PMID:1080748", "title": "Comparison of 51Cr release and microcytotoxicity assays against human melanoma cells.", "content": "Comparisons of the cytotoxic activity of mononuclear cells from venous blood of human subjects against human melanoma cell lines have been made by means of the 51Cr release assay (CRA) and the microcytotoxicity assay (MCA) of Takasugi and Klein. The relative effectiveness of the two assays in the detection of killing by various sub-populations of effector cells was studied after depletion of monocytes and separation of the blood monocluear cells by SRBC receptor and Fc receptor resetting techniques. Overall, the results showed good correlation. Most differences were the result of low values recorded with the CRA when effector cells were obtained from Hypaque-Ficoll--separated populations which had not been further fractionated. These differences were largely removed when monocytes were depleted by glass absorption and may reflect interference with the CRA by monocytes. No consistent differences were found between the assays in the detection of killing by subfractions enriched for T cells or B cells in this tumour system. The MCA appears to offer the advantage of greater sensitivity and possibly detection of a wider range of cytotoxic mechanisms but the CRA has a number of advantages of a technical nature which make it more applicable to routine assays in clinical studies.", "contents": "Comparison of 51Cr release and microcytotoxicity assays against human melanoma cells. Comparisons of the cytotoxic activity of mononuclear cells from venous blood of human subjects against human melanoma cell lines have been made by means of the 51Cr release assay (CRA) and the microcytotoxicity assay (MCA) of Takasugi and Klein. The relative effectiveness of the two assays in the detection of killing by various sub-populations of effector cells was studied after depletion of monocytes and separation of the blood monocluear cells by SRBC receptor and Fc receptor resetting techniques. Overall, the results showed good correlation. Most differences were the result of low values recorded with the CRA when effector cells were obtained from Hypaque-Ficoll--separated populations which had not been further fractionated. These differences were largely removed when monocytes were depleted by glass absorption and may reflect interference with the CRA by monocytes. No consistent differences were found between the assays in the detection of killing by subfractions enriched for T cells or B cells in this tumour system. The MCA appears to offer the advantage of greater sensitivity and possibly detection of a wider range of cytotoxic mechanisms but the CRA has a number of advantages of a technical nature which make it more applicable to routine assays in clinical studies."}
{"id": "PMID:1080749", "title": "A study of the oncogenicity of adenovirus type 2 transformed rat embryo cells.", "content": "Rat embryo cells were transformed by adenovirus type 2 at different multiplicities of infection. The oncogenic potential of the resulting transformed lines varied considerably but a relationship was found to exist between transforming virus dose and oncogenicity of the resulting transformed lines. Possible reasons for the considerable variation in oncogenicity of the transformed lines are discussed, giving particular consideration to cellular antigenicity and recent molecular biology studies of adenovirus type 2 transformed cell lines.", "contents": "A study of the oncogenicity of adenovirus type 2 transformed rat embryo cells. Rat embryo cells were transformed by adenovirus type 2 at different multiplicities of infection. The oncogenic potential of the resulting transformed lines varied considerably but a relationship was found to exist between transforming virus dose and oncogenicity of the resulting transformed lines. Possible reasons for the considerable variation in oncogenicity of the transformed lines are discussed, giving particular consideration to cellular antigenicity and recent molecular biology studies of adenovirus type 2 transformed cell lines."}
{"id": "PMID:1080750", "title": "Specific anti-tumor responses of cultured immune spleen cells III. Further characterization of cells which synthesize factors with blocking and antiserum-dependent cellular cytotoxic (ADC) activities.", "content": "Spleen cells from BALB/c mice bearing syngeneic, methylcholanthrene-induced sarcomas were cultured in vitro. In agreement with previous observations, two tumor-specific activities could be demonstrated in the culture supernatants: the supernatants suppressed (blocked) specific lymph-node cell-mediated cytotoxicity directed against the respective neoplasm and they induced specific antibody-dependent-cellular cytotoxicity (ADC) mediated by lymph-node cells from non-immune mice. In the present study, the effects of specific depletion or enrichment of different celltypes on the ability to synthesize the blocking and ADC activities was explored. Spleen cells passed through columns of Sephadex G-10, which removed plasma cells and macrophages, no longer synthesized factors with ADC activity though synthesis of blocking factors continued. However, lysis of Thy-1-positive cells with antiserum and complement did abolish the synthesis of blocking factors. Spleen cells from tumor-bearing mice were then enriched for Thy-1-positive cells by selective removal of macrophages and plasma cells on G-10 columns and of immunoglobulin-bearing cells on anti-mouse immunoglobulin affinity columns. This T-cell-enriched population synthesized blocking factors and restored blocking factor synthesis in cultures depleted of Thy-1-positive cells. Simiarly enriched spleen cells from normal control mice did not synthesize tumor-specific blocking factors but partially restored synthesis of blocking factor in tumor-bearer spleen cultures depleted of Thy-1-positive cells.", "contents": "Specific anti-tumor responses of cultured immune spleen cells III. Further characterization of cells which synthesize factors with blocking and antiserum-dependent cellular cytotoxic (ADC) activities. Spleen cells from BALB/c mice bearing syngeneic, methylcholanthrene-induced sarcomas were cultured in vitro. In agreement with previous observations, two tumor-specific activities could be demonstrated in the culture supernatants: the supernatants suppressed (blocked) specific lymph-node cell-mediated cytotoxicity directed against the respective neoplasm and they induced specific antibody-dependent-cellular cytotoxicity (ADC) mediated by lymph-node cells from non-immune mice. In the present study, the effects of specific depletion or enrichment of different celltypes on the ability to synthesize the blocking and ADC activities was explored. Spleen cells passed through columns of Sephadex G-10, which removed plasma cells and macrophages, no longer synthesized factors with ADC activity though synthesis of blocking factors continued. However, lysis of Thy-1-positive cells with antiserum and complement did abolish the synthesis of blocking factors. Spleen cells from tumor-bearing mice were then enriched for Thy-1-positive cells by selective removal of macrophages and plasma cells on G-10 columns and of immunoglobulin-bearing cells on anti-mouse immunoglobulin affinity columns. This T-cell-enriched population synthesized blocking factors and restored blocking factor synthesis in cultures depleted of Thy-1-positive cells. Simiarly enriched spleen cells from normal control mice did not synthesize tumor-specific blocking factors but partially restored synthesis of blocking factor in tumor-bearer spleen cultures depleted of Thy-1-positive cells."}
{"id": "PMID:1080751", "title": "Antibody-dependent cell-mediated cytotoxicity for human monolayer target cells bearing blood group and transplantation antigens and for melanoma cells.", "content": "A sensitive assay for antibody-dependent cell-mediated cytotoxicity (ADCMC) was developed utilizing serum from a patient with gestational choriocarcinoma and the 3H-proline microcytotoxicity test for detection fo destruction of monolayer target cells. Conditions for optimal expression of ADCMC were investigated using serum from this patient and skin fibroblasts from her husband and daugther as target cells with semi-purified blood leukocytes from normal donors as effector cells. Factors critical for optimal expression of ADCMC in this assay are the selection of effector cell donors possessing high levels of activity in the presence of serum with known lymphocyte-dependent antibody. (LDA), the effector cell preparative technique, and  incubation for up to 40 h. Tris-NH4Cl lysis of red blood cells was found significantly to reduce effector cell activity. Under optimal conditions, the LDA titer of this patient's serum was greater than 10(-4). The sensitivity of the assay was confirmed by the detection of LDA activity of an anti-blood-group antibody at dilutions not demonstrating hemagglutination, and by the induction of ADCMC for blood group A antigen-bearing target cells by normal sera of B and O blood groups. In a preliminary study of sera from 16 melanoma patients on the corresponding antologous tumor target cells, four had significant LDA. Further studies are under way to determine specificty, incidence, and relationship of LDA-positive autologous sera to course of disease.", "contents": "Antibody-dependent cell-mediated cytotoxicity for human monolayer target cells bearing blood group and transplantation antigens and for melanoma cells. A sensitive assay for antibody-dependent cell-mediated cytotoxicity (ADCMC) was developed utilizing serum from a patient with gestational choriocarcinoma and the 3H-proline microcytotoxicity test for detection fo destruction of monolayer target cells. Conditions for optimal expression of ADCMC were investigated using serum from this patient and skin fibroblasts from her husband and daugther as target cells with semi-purified blood leukocytes from normal donors as effector cells. Factors critical for optimal expression of ADCMC in this assay are the selection of effector cell donors possessing high levels of activity in the presence of serum with known lymphocyte-dependent antibody. (LDA), the effector cell preparative technique, and  incubation for up to 40 h. Tris-NH4Cl lysis of red blood cells was found significantly to reduce effector cell activity. Under optimal conditions, the LDA titer of this patient's serum was greater than 10(-4). The sensitivity of the assay was confirmed by the detection of LDA activity of an anti-blood-group antibody at dilutions not demonstrating hemagglutination, and by the induction of ADCMC for blood group A antigen-bearing target cells by normal sera of B and O blood groups. In a preliminary study of sera from 16 melanoma patients on the corresponding antologous tumor target cells, four had significant LDA. Further studies are under way to determine specificty, incidence, and relationship of LDA-positive autologous sera to course of disease."}
{"id": "PMID:1080752", "title": "The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells.", "content": "The TE-85 osteosarcoma cell line has several of the in vitro properties of malignant cells, including colony formation in agar, but has low extracellular fibrinolytic activity and no capacity to form tumors in ATS-treated hamsters. Some TE-85 cells clones (clones 2, 4 and 6) have increased fibrinolytic activity but do not form tumors in hamsters. TE-85 cells infected with mammalian transformation-defective viruses have low (FeLV) or increased (RD-114 virus) levels of fibrinolytic activity and do not form tumors in hamsters. TE-85 cell either nonproductively infected with Ki-MSV or productively infected with M-MSV (RD-114), have fibrinolytic activity and can form tumors in hamsters. The MSV gene(s) but not colony formation in agar or extracellular fibrinolytic activity appears to be capable of rendering TE-85 cells tumorigenic in ATS-treated hamsters.", "contents": "The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells. The TE-85 osteosarcoma cell line has several of the in vitro properties of malignant cells, including colony formation in agar, but has low extracellular fibrinolytic activity and no capacity to form tumors in ATS-treated hamsters. Some TE-85 cells clones (clones 2, 4 and 6) have increased fibrinolytic activity but do not form tumors in hamsters. TE-85 cells infected with mammalian transformation-defective viruses have low (FeLV) or increased (RD-114 virus) levels of fibrinolytic activity and do not form tumors in hamsters. TE-85 cell either nonproductively infected with Ki-MSV or productively infected with M-MSV (RD-114), have fibrinolytic activity and can form tumors in hamsters. The MSV gene(s) but not colony formation in agar or extracellular fibrinolytic activity appears to be capable of rendering TE-85 cells tumorigenic in ATS-treated hamsters."}
{"id": "PMID:1080753", "title": "Rejection of tumor cells in vitro: a T-cell-mediated reaction.", "content": "Cytoxic lymphoblasts (LBC) were produced by the co-cultivation of guinea-pig lymphoid cells from the thymus, lymph nodes, spleen, bone marrow and blood with syngeneic methylcholanthrene-induced sarcoma (MC-D) cells. The cytotoxic reaction was inhibited by pretreatment of the lymphoid cell populations with a rabbit antiserum specific for guinea-pig thymus-derived (T) lymphocytes. Supernatants of LBC inhibited the migration of peritoneal exudate cells, but did not contain lymphotoxin or a skin reactive factor. It is concluded that the cytotoxic cells are of T-cell origin.", "contents": "Rejection of tumor cells in vitro: a T-cell-mediated reaction. Cytoxic lymphoblasts (LBC) were produced by the co-cultivation of guinea-pig lymphoid cells from the thymus, lymph nodes, spleen, bone marrow and blood with syngeneic methylcholanthrene-induced sarcoma (MC-D) cells. The cytotoxic reaction was inhibited by pretreatment of the lymphoid cell populations with a rabbit antiserum specific for guinea-pig thymus-derived (T) lymphocytes. Supernatants of LBC inhibited the migration of peritoneal exudate cells, but did not contain lymphotoxin or a skin reactive factor. It is concluded that the cytotoxic cells are of T-cell origin."}
{"id": "PMID:1080754", "title": "Reduction in T-lymphocytes forming active rosettes in chronic marijuana smokers.", "content": "Rosettes formed by peripheral T- and B-lymphocytes were studied in 23 normal controls and 23 marijuana smokers. The mean percentage of cells forming B type rosettes were normal in both groups. The percentage of T cells forming rosettes was lower in the marijuana smokers, and 39% had values below 2 standard deviations of the mean of the normal control group. These observations suggest some alteration in some T cells in some marijuana smokers.", "contents": "Reduction in T-lymphocytes forming active rosettes in chronic marijuana smokers. Rosettes formed by peripheral T- and B-lymphocytes were studied in 23 normal controls and 23 marijuana smokers. The mean percentage of cells forming B type rosettes were normal in both groups. The percentage of T cells forming rosettes was lower in the marijuana smokers, and 39% had values below 2 standard deviations of the mean of the normal control group. These observations suggest some alteration in some T cells in some marijuana smokers."}
{"id": "PMID:1080760", "title": "Mechanism of gap-filling during postreplication repair of ultraviolet damage in Haemophilus influenzae.", "content": "Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling process is controlled by the rec-1 gene. Gap-filling occurs normally in a temperature-sensitive DNA synthesis mutant at the restrictive temperature showing that normal semiconservative DNA synthesis is not necessary for gap-filling. To test for recombinational events after irradiation, the DNA synthesized after irradiation was radioactively labeled for a short time in medium containing 5-bromodeoxyuridine followed by incubation for various times in non-radioactive, 5-bromodeoxyuridine-containing medium. The DNA was denatured and analyzed isopycnically. The labeled DNA was initially \"heavy,\" but later shifted toward lighter densities. This shift occurred in the temperature-sensitive DNA synthesis mutant at the restrictive temperature and in the recombination-defective mutant rec-2, but was not seen in the rec-1 mutant. The density shift can be interpreted as evidence that rather extensive exchanges occurred between parental DNA and the DNA made after irradiation. These results suggest that such exchanges are important for gap-filling in H. influenzae.", "contents": "Mechanism of gap-filling during postreplication repair of ultraviolet damage in Haemophilus influenzae. Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling process is controlled by the rec-1 gene. Gap-filling occurs normally in a temperature-sensitive DNA synthesis mutant at the restrictive temperature showing that normal semiconservative DNA synthesis is not necessary for gap-filling. To test for recombinational events after irradiation, the DNA synthesized after irradiation was radioactively labeled for a short time in medium containing 5-bromodeoxyuridine followed by incubation for various times in non-radioactive, 5-bromodeoxyuridine-containing medium. The DNA was denatured and analyzed isopycnically. The labeled DNA was initially \"heavy,\" but later shifted toward lighter densities. This shift occurred in the temperature-sensitive DNA synthesis mutant at the restrictive temperature and in the recombination-defective mutant rec-2, but was not seen in the rec-1 mutant. The density shift can be interpreted as evidence that rather extensive exchanges occurred between parental DNA and the DNA made after irradiation. These results suggest that such exchanges are important for gap-filling in H. influenzae."}
{"id": "PMID:1080761", "title": "Mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. Proton nuclear magnetic resonance studies of the binding of adenine nucleosides and substrate to ethanolamine ammonia-lyase.", "content": "Proton NMR spectroscopy was used to study the binding of adenosine, 5'-deoxyadenosine, adenine, and ethanolamine to the adenosylcobalamin-dependent enzyme ethanolamine ammonia-lyase. Broadening of ligand resonances in the presence of ethanolamine ammonia-lyase indicated that adenosine, 5'-deoxyadenosine, and ethanolamine all formed complexes with the enzyme (KD(mM) = 3.5, 3.0, and 2.5 respectively). The methyl group of enzyme-bound 5'-deoxyadenosine rotated at a rate exceeding 10(7) revolutions/s. Adenine did not appear to bind to the enzyme. Rates of dissociation of nucleosides from the enzyme were fast on the NMR time scale, precluding measurements of rate constants for the binding reaction. The departure of ethanolamine was slow, however, permitting their determination. The values for these rate constants were: k1 = 4.4 times 10(5) M-1 S-1; k-1 = 1.1 times 10(3) S-1. Addition of 1 mol of cyanocobalamin/mol of active sites led to narrowing of the enzyme-broadened ligand resonances. With 5'-deoxyadenosine, linewidths still exceeded those of the free ligand, indicating that binding to enzyme was weakened but not abolished. The KD for this nucleoside in the presence of CNCbl was 8.0 mM. With ethanolamine and adenosine, however, linewidths reverted to values characteristic of the unbound ligand, indicating either that CNCbl greatly lowered the rate of dissociation of the ligand or displaced the ligand from the enzyme. A decision between these two possibilities could not be made from the data at hand, although analogy with the situation obtaining with 5'-deoxyadenosine suggests that adenosine is displaced from the enzyme by CNCbl. 5'-Deoxyadenosine inhibited catalytic activity of the enzyme, competing with adenosylcobalamin (Ki = 2.7 mM). Adenosine had no effect, despite NMR evidence indicating that it formed a complex with free enzyme. These experiments showed that ethanolamine ammonia-lyase possesses binding sites for adenine nucleosides, a class of compounds chemically related to the Cobeta-ligand of the cofactor, as well as for ethanolamine. Binding to the enzyme has now been demonstrated for all three categories of low molecular weight compounds thought to be involved in the reaction; namely, substrate (ethanolamine), corrin, and adenine nucleoside.", "contents": "Mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. Proton nuclear magnetic resonance studies of the binding of adenine nucleosides and substrate to ethanolamine ammonia-lyase. Proton NMR spectroscopy was used to study the binding of adenosine, 5'-deoxyadenosine, adenine, and ethanolamine to the adenosylcobalamin-dependent enzyme ethanolamine ammonia-lyase. Broadening of ligand resonances in the presence of ethanolamine ammonia-lyase indicated that adenosine, 5'-deoxyadenosine, and ethanolamine all formed complexes with the enzyme (KD(mM) = 3.5, 3.0, and 2.5 respectively). The methyl group of enzyme-bound 5'-deoxyadenosine rotated at a rate exceeding 10(7) revolutions/s. Adenine did not appear to bind to the enzyme. Rates of dissociation of nucleosides from the enzyme were fast on the NMR time scale, precluding measurements of rate constants for the binding reaction. The departure of ethanolamine was slow, however, permitting their determination. The values for these rate constants were: k1 = 4.4 times 10(5) M-1 S-1; k-1 = 1.1 times 10(3) S-1. Addition of 1 mol of cyanocobalamin/mol of active sites led to narrowing of the enzyme-broadened ligand resonances. With 5'-deoxyadenosine, linewidths still exceeded those of the free ligand, indicating that binding to enzyme was weakened but not abolished. The KD for this nucleoside in the presence of CNCbl was 8.0 mM. With ethanolamine and adenosine, however, linewidths reverted to values characteristic of the unbound ligand, indicating either that CNCbl greatly lowered the rate of dissociation of the ligand or displaced the ligand from the enzyme. A decision between these two possibilities could not be made from the data at hand, although analogy with the situation obtaining with 5'-deoxyadenosine suggests that adenosine is displaced from the enzyme by CNCbl. 5'-Deoxyadenosine inhibited catalytic activity of the enzyme, competing with adenosylcobalamin (Ki = 2.7 mM). Adenosine had no effect, despite NMR evidence indicating that it formed a complex with free enzyme. These experiments showed that ethanolamine ammonia-lyase possesses binding sites for adenine nucleosides, a class of compounds chemically related to the Cobeta-ligand of the cofactor, as well as for ethanolamine. Binding to the enzyme has now been demonstrated for all three categories of low molecular weight compounds thought to be involved in the reaction; namely, substrate (ethanolamine), corrin, and adenine nucleoside."}
{"id": "PMID:1080762", "title": "Nucleocytoplasmic transport and distribution of an amino acid, in situ.", "content": "Ultra-low temperature techniques (microdissection and autoradiography) were used to study the nucleocytoplasmic distribution and transport of alpha-aminoisobutyric acid (AIB) in an amino acid-accumulating cell. In amphibiam oocytes incubated in AIB, the nuclear concentration of this non-metabolizable  amino acid exceeds the cytoplasmic concentration by 45%, remaining constant both over time and variation in substrate concentration. The kinetics of uptake suggest that this nucleo-cytoplasmic asymmetry arises from solubility differences between the 2 compartments, and that the nuclear envelope plays a negligible role in amino acid transport. A solute exclusion model is offered to explain the nucleocytoplasmic asymmetry.", "contents": "Nucleocytoplasmic transport and distribution of an amino acid, in situ. Ultra-low temperature techniques (microdissection and autoradiography) were used to study the nucleocytoplasmic distribution and transport of alpha-aminoisobutyric acid (AIB) in an amino acid-accumulating cell. In amphibiam oocytes incubated in AIB, the nuclear concentration of this non-metabolizable  amino acid exceeds the cytoplasmic concentration by 45%, remaining constant both over time and variation in substrate concentration. The kinetics of uptake suggest that this nucleo-cytoplasmic asymmetry arises from solubility differences between the 2 compartments, and that the nuclear envelope plays a negligible role in amino acid transport. A solute exclusion model is offered to explain the nucleocytoplasmic asymmetry."}
{"id": "PMID:1080765", "title": "Detection of adenovirus in patient specimens by indirect immune electron microscopy.", "content": "Immune electron microscopic procedures for the detection of adenovirus type 7 directly in throat swab specimens from patients are described. Nineteen of 25 throat swab samples, known to be positive for adenovirus type 7 by isolation of virus from tissue culture, were shown to contain aggregates of adenovirions coated with antibody. Sensitivity tests of the immune electron microscopic method showed that as few as 16 to 32 tissue culture infective doses of virus could be detected by the direct immune electron microscopic technique. It was also demonstrated that aggregation of virus-antibody complexes could be further enhanced by use of anti-immunoglobulin G sera (indirect immune electron microscopic procedures). These results demonstrate that examination of patient specimen by immune electron microscopic procedures is a feasible and rapid method for adenovirus detection and suggest that it could be applied as a routine laboratory procedure for the diagnosis of other virus infections.", "contents": "Detection of adenovirus in patient specimens by indirect immune electron microscopy. Immune electron microscopic procedures for the detection of adenovirus type 7 directly in throat swab specimens from patients are described. Nineteen of 25 throat swab samples, known to be positive for adenovirus type 7 by isolation of virus from tissue culture, were shown to contain aggregates of adenovirions coated with antibody. Sensitivity tests of the immune electron microscopic method showed that as few as 16 to 32 tissue culture infective doses of virus could be detected by the direct immune electron microscopic technique. It was also demonstrated that aggregation of virus-antibody complexes could be further enhanced by use of anti-immunoglobulin G sera (indirect immune electron microscopic procedures). These results demonstrate that examination of patient specimen by immune electron microscopic procedures is a feasible and rapid method for adenovirus detection and suggest that it could be applied as a routine laboratory procedure for the diagnosis of other virus infections."}
{"id": "PMID:1080763", "title": "[Leiomyoma of Meckel's diverticulum revealed by a digestive hemorrhage. Preoperative diagnosis by selective arteriography].", "content": "The authors report a case revealed by low digestive hemorrhage, and in the light of cases in the literature, discuss the various problems due to leiomyomas in Meckel's diverticulum. They emphasize particularly the risk of late diagnosis at the stage of leiomyosarcoma. As it is impossible to say afterwards whether a muscular tumour of the digestive tract is benign or not, it is important to make an early diagnosis. Arteriography is one of the best means.", "contents": "[Leiomyoma of Meckel's diverticulum revealed by a digestive hemorrhage. Preoperative diagnosis by selective arteriography]. The authors report a case revealed by low digestive hemorrhage, and in the light of cases in the literature, discuss the various problems due to leiomyomas in Meckel's diverticulum. They emphasize particularly the risk of late diagnosis at the stage of leiomyosarcoma. As it is impossible to say afterwards whether a muscular tumour of the digestive tract is benign or not, it is important to make an early diagnosis. Arteriography is one of the best means."}
{"id": "PMID:1080766", "title": "Obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis).", "content": "Six obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis) have been isolated and their characteristics studied. The reactions of the anaerobic strains, as well as of facultative strains tested under anaerobic conditions, are similar to the reactions previously reported for this species.", "contents": "Obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis). Six obligately anaerobic strains of Corynebacterium vaginale (Haemophilus vaginalis) have been isolated and their characteristics studied. The reactions of the anaerobic strains, as well as of facultative strains tested under anaerobic conditions, are similar to the reactions previously reported for this species."}
{"id": "PMID:1080767", "title": "Problems in the detection of carriers of haemophilia A.", "content": "Factor VIII activity and factor VIII related--or Willebrand--antigen were studied in 49 known carriers of haemophilia A and 31 normal women, and the data were analysed by four statistical approaches. Sixteen per cent of normals and 18% of carriers were misclassified, overlapping with the other group. Although the percentage of carriers detected is higher when taking into account the results of both biological and immunological factor VIII, it is lower than others recently reported, and the discrepancies between the results obtained are discussed.", "contents": "Problems in the detection of carriers of haemophilia A. Factor VIII activity and factor VIII related--or Willebrand--antigen were studied in 49 known carriers of haemophilia A and 31 normal women, and the data were analysed by four statistical approaches. Sixteen per cent of normals and 18% of carriers were misclassified, overlapping with the other group. Although the percentage of carriers detected is higher when taking into account the results of both biological and immunological factor VIII, it is lower than others recently reported, and the discrepancies between the results obtained are discussed."}
{"id": "PMID:1080768", "title": "Unmyelinated preganglionic fibers in frog ventral roots.", "content": "It has been suggested that the preganglionic visceral fibers in the frog are unmyelinated. To obtain further evidence for this suggestion: (1) the percentages of unmyelinated fibers and the myelinated fiber histograms were done for all frog ventral roots, (2) the number of unmyelinated fibers were counted on either side of a chronic section of the seventh ventral root and (3) compound action potentials were obtained for roots 7-10. The results are that slightly more than 80% of the unmyelinated fibers in the seventh ventral root arise in the spinal cord and are presumably unmyelinated preganglionic efferents. Furthermore, a C fiber volley could be followed from the seventh ventral root into caudal parts of the sympathetic chain. Thus this data provides confirmation for the idea that many frog preganglionic fibers are unmyelinated. This study also shows that 24% of the ventral root axons in the frog are unmyelinated and that, on the basis of percentages of unmyelinated fibers and myelinated fiber histograms, there are five categories of frog ventral roots.", "contents": "Unmyelinated preganglionic fibers in frog ventral roots. It has been suggested that the preganglionic visceral fibers in the frog are unmyelinated. To obtain further evidence for this suggestion: (1) the percentages of unmyelinated fibers and the myelinated fiber histograms were done for all frog ventral roots, (2) the number of unmyelinated fibers were counted on either side of a chronic section of the seventh ventral root and (3) compound action potentials were obtained for roots 7-10. The results are that slightly more than 80% of the unmyelinated fibers in the seventh ventral root arise in the spinal cord and are presumably unmyelinated preganglionic efferents. Furthermore, a C fiber volley could be followed from the seventh ventral root into caudal parts of the sympathetic chain. Thus this data provides confirmation for the idea that many frog preganglionic fibers are unmyelinated. This study also shows that 24% of the ventral root axons in the frog are unmyelinated and that, on the basis of percentages of unmyelinated fibers and myelinated fiber histograms, there are five categories of frog ventral roots."}
{"id": "PMID:1080769", "title": "An investigation of the family background of acute Haemophilus infections of children.", "content": "Nose and throat swabs, for culture of Haemophilus influenza type b, and blood samples, for measurement of antibodies specific for that serotype, were collected from members of 28 families from which children had been admitted to hospital with acute H. influenzae type b infections (mainly meningitis or epiglottitis). The patients with meningitis were younger than those with epiglottitis and had more siblings, with a marked predominance of sisters. Investigations within a few days of admission of the affected children to hospital detected carriers of H. influenzae type b (19 altogether) in 13 of the 28 families, including 9 of the 13 families with 3 or more children. Members with raised antibody titres for H. influenzae type b (suggesting the presence of the organism for at least a few weeks) were found in 17 of the 25 families from which blood samples were obtained, including all 11 families with 3 or more children. Most of the patients probably acquired their infections from within their own families, and siblings under 11 years old were of predominant importance both as carriers and as potential sources of the patients' infections. Persistence of the organism within families for up to 6 months was demonstrated. Possible reasons for the difference in age-incidence between haemophilus meningitis and epiglottitis and for the occurrence of the former in babies with older sisters are suggested, and also a possible connection between the results of this survey and the likely value of immunization against H. influenzae type b.", "contents": "An investigation of the family background of acute Haemophilus infections of children. Nose and throat swabs, for culture of Haemophilus influenza type b, and blood samples, for measurement of antibodies specific for that serotype, were collected from members of 28 families from which children had been admitted to hospital with acute H. influenzae type b infections (mainly meningitis or epiglottitis). The patients with meningitis were younger than those with epiglottitis and had more siblings, with a marked predominance of sisters. Investigations within a few days of admission of the affected children to hospital detected carriers of H. influenzae type b (19 altogether) in 13 of the 28 families, including 9 of the 13 families with 3 or more children. Members with raised antibody titres for H. influenzae type b (suggesting the presence of the organism for at least a few weeks) were found in 17 of the 25 families from which blood samples were obtained, including all 11 families with 3 or more children. Most of the patients probably acquired their infections from within their own families, and siblings under 11 years old were of predominant importance both as carriers and as potential sources of the patients' infections. Persistence of the organism within families for up to 6 months was demonstrated. Possible reasons for the difference in age-incidence between haemophilus meningitis and epiglottitis and for the occurrence of the former in babies with older sisters are suggested, and also a possible connection between the results of this survey and the likely value of immunization against H. influenzae type b."}
{"id": "PMID:1080770", "title": "Regulatory substances produced by lymphocytes. II. Lymphotoxin in the rat.", "content": "Rat lymphotoxin (LT), a soluble mediator which kills L cells (mouse fibroblasts) within 48 hr, was partially purified by conventional protein fractionation procedures from the culture supernatants of rat lymph node cells stimulated with ovalbumin in vivo and rechallenged with the same antigen in vitro. It appeared, from the elution pattern of DEAE-cellulose chromatography, to be a neutral or slightly basic protein and was inactivated at 60 degrees C. Its molecular weight was estimated by Sephadex gel filtration to be approximately 9 times 10(4) daltons. Partially purified LT, in titration studies, showed single hit kinetics. It also inhibited L cell proliferation at low concentration without significant killing effect. The heat inactivation curves of both cytotoxic and proliferation inhibitory activities were the same, suggesting that these two activities may be caused by the same molecule. However, it had almost no detectable effect on the DNA synthesis of rat lymph node cells stimulated with phytohemagglutinin (PHA), suggesting that lymphocytes, at least those which respond to HA, are rather resistant to LT. It is suggested that activities described as \"proliferation inhibitory factor\" and \"cloning inhibitory factor\" by others may represent the activity of dilute LT. The \"inhibitor of DNA synthesis\" is distinct in molecular character, range of target cells, and target cell kinetics from LT.", "contents": "Regulatory substances produced by lymphocytes. II. Lymphotoxin in the rat. Rat lymphotoxin (LT), a soluble mediator which kills L cells (mouse fibroblasts) within 48 hr, was partially purified by conventional protein fractionation procedures from the culture supernatants of rat lymph node cells stimulated with ovalbumin in vivo and rechallenged with the same antigen in vitro. It appeared, from the elution pattern of DEAE-cellulose chromatography, to be a neutral or slightly basic protein and was inactivated at 60 degrees C. Its molecular weight was estimated by Sephadex gel filtration to be approximately 9 times 10(4) daltons. Partially purified LT, in titration studies, showed single hit kinetics. It also inhibited L cell proliferation at low concentration without significant killing effect. The heat inactivation curves of both cytotoxic and proliferation inhibitory activities were the same, suggesting that these two activities may be caused by the same molecule. However, it had almost no detectable effect on the DNA synthesis of rat lymph node cells stimulated with phytohemagglutinin (PHA), suggesting that lymphocytes, at least those which respond to HA, are rather resistant to LT. It is suggested that activities described as \"proliferation inhibitory factor\" and \"cloning inhibitory factor\" by others may represent the activity of dilute LT. The \"inhibitor of DNA synthesis\" is distinct in molecular character, range of target cells, and target cell kinetics from LT."}
{"id": "PMID:1080771", "title": "An analysis of the defective response of CBA/N mice to T-dependent antigens.", "content": "CBA/N mice fail to respond to T-independent antigens, and give a diminished response to T-dependent antigens. The cellular basis of the diminished response to T-dependent antigens in this strain was analyzed by means of adoptive transfers of various primed cell populations along with cells from the immunologically normal, histocompatible strain CBA/CaJ. CBA/N mice behaved normally as recipients and had normal levels of spleen helper cell activity. However, CBA/N spleen cells, used as a B cell source, produced only 56% as much antibody as CBA/CaJ cells. Since CBA/N mice have a relative frequency of immunoglobulin-bearing lymphocytes roughly half that of normal mice, these results suggest that CBA/N mice have a quantitative defect in spleen B cells responsive to T-dependent antigens.", "contents": "An analysis of the defective response of CBA/N mice to T-dependent antigens. CBA/N mice fail to respond to T-independent antigens, and give a diminished response to T-dependent antigens. The cellular basis of the diminished response to T-dependent antigens in this strain was analyzed by means of adoptive transfers of various primed cell populations along with cells from the immunologically normal, histocompatible strain CBA/CaJ. CBA/N mice behaved normally as recipients and had normal levels of spleen helper cell activity. However, CBA/N spleen cells, used as a B cell source, produced only 56% as much antibody as CBA/CaJ cells. Since CBA/N mice have a relative frequency of immunoglobulin-bearing lymphocytes roughly half that of normal mice, these results suggest that CBA/N mice have a quantitative defect in spleen B cells responsive to T-dependent antigens."}
{"id": "PMID:1080772", "title": "Antigenic analysis of Chlamydiae by two-dimensional immunoelectrophoresis. I. Antigenic heterogeneity between C. trachomatis and C. psittaci.", "content": "Tw0-dimensional immunoelectrophoresis was used for the antigenic analysis of Chlamydia. Antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent Triton X-100. Nineteen and 16 distinct components were observed for Chlamydia trachomatis strain L2/434/Bu and C. psittaci strain Meningopneumonitis/Cal-10, respectively. The triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed between 434 and Cal-10. However, the antigens of Trachoma strain K/UW-31/Cx and LGV strain 434 were found to be very similar.", "contents": "Antigenic analysis of Chlamydiae by two-dimensional immunoelectrophoresis. I. Antigenic heterogeneity between C. trachomatis and C. psittaci. Tw0-dimensional immunoelectrophoresis was used for the antigenic analysis of Chlamydia. Antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent Triton X-100. Nineteen and 16 distinct components were observed for Chlamydia trachomatis strain L2/434/Bu and C. psittaci strain Meningopneumonitis/Cal-10, respectively. The triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed between 434 and Cal-10. However, the antigens of Trachoma strain K/UW-31/Cx and LGV strain 434 were found to be very similar."}
{"id": "PMID:1080773", "title": "Triggering mechanism of B lymphocytes. I. Effect of anti-immunoglobulin and enhancing soluble factor on differentiation and proliferation of B cells.", "content": "Stimulation of rabbit mesenteric lymphocytes with anti-immunoglobulin antibody (anti-Ig) for 24 hr (first stage of culture) followed by the addition of enhancing soluble factor (second stage of culture) induced the formation of IgG. The presence of mitotic inhibitors, such as cytosine arabinoside (Ara-C), hydroxyurea (HU), with anti-Ig for the first 24 hr of incubation did not inhibit the production of IgG induced by anti-Ig and soluble factor. The addition of cytochalasin-B in the first stage of culture with anti-Ig did not inhibit, but rather enhanced, the formation of IgG. On the other hand, the presence of Ara-C, HU, or cytochalasin-B with soluble factor in the second stage of culture completely inhibited the production of IgG. Soluble factor preferentially induced the uptake of thymidine of anti-Ig-stimulated cells. Lipopolysaccharide (LPS) also induced the proliferation of rabbit lymphocytes, but did not induce the IgG production of anti-Ig-stimulated cells. However, LPS-stimulated cells formed a significant amounf of IgG when they were cultured with soluble factor. Anti-Ig-stimulated cells absorbed the enhancing activity of soluble factor, but non-stimulated cells did not absorb the activity. These results showed that cross-linkage of Ig receptors or LPS receptors with anti-Ig or LPS induced the formation of acceptor sites for soluble factor without the requirement of cell division and these activated cells started to proliferate and maturate to antibody-forming cells under the influence of soluble factor.", "contents": "Triggering mechanism of B lymphocytes. I. Effect of anti-immunoglobulin and enhancing soluble factor on differentiation and proliferation of B cells. Stimulation of rabbit mesenteric lymphocytes with anti-immunoglobulin antibody (anti-Ig) for 24 hr (first stage of culture) followed by the addition of enhancing soluble factor (second stage of culture) induced the formation of IgG. The presence of mitotic inhibitors, such as cytosine arabinoside (Ara-C), hydroxyurea (HU), with anti-Ig for the first 24 hr of incubation did not inhibit the production of IgG induced by anti-Ig and soluble factor. The addition of cytochalasin-B in the first stage of culture with anti-Ig did not inhibit, but rather enhanced, the formation of IgG. On the other hand, the presence of Ara-C, HU, or cytochalasin-B with soluble factor in the second stage of culture completely inhibited the production of IgG. Soluble factor preferentially induced the uptake of thymidine of anti-Ig-stimulated cells. Lipopolysaccharide (LPS) also induced the proliferation of rabbit lymphocytes, but did not induce the IgG production of anti-Ig-stimulated cells. However, LPS-stimulated cells formed a significant amounf of IgG when they were cultured with soluble factor. Anti-Ig-stimulated cells absorbed the enhancing activity of soluble factor, but non-stimulated cells did not absorb the activity. These results showed that cross-linkage of Ig receptors or LPS receptors with anti-Ig or LPS induced the formation of acceptor sites for soluble factor without the requirement of cell division and these activated cells started to proliferate and maturate to antibody-forming cells under the influence of soluble factor."}
{"id": "PMID:1080774", "title": "Triggering mechanism of B lymphocytes. II. Induction of ornithine decarboxylase in B cells by anti-immunoglobulin and enhancing soluble factor.", "content": "In vitro stimulation of rabbit lymphocytes with anti-immunoglobulin antibody (anti-Ig) induced ornithine decarboxylase (ODC) (EC4.1.1.17) in the cells. The enzyme activity reached the maximum at 8 hr after stimulation of the cell with antibody and returned to the unstimulated level after 20 hr. The optimum concentration of antibody to produce the maximum activity of ODC was 25 mug/ml, which was in the same range that required for the maximum production of IgG and the highest rate of DNA synthesis. ODC was again induced with soluble factor (SF) in the cells that had been previously treated with anti-Ig antibody for 24 hr. The treatment of the cells with anti-thymocyte serum and complement did not affect the activity of the enzyme induced by anti-Ig or by SF, indicating that enzyme induction was an event occurring in B cells and not in T cells. The addition of mitotic inhibitors, such as hydroxy-urea (HU) or cytosine arabinoside (Ara-C), to the culture with anti-Ig did not inhibit the production of ODC. The presence of cytochalasin-B with anti-Ig did not inhibit, but rather enhanced, the synthesis of ODC. On the other hand, the presence of HU or cytochalasin-B with soluble factor completely suppressed the induction ODC. These results suggested that intracellular events that trigger the induction of ODC by the interaction of Ig receptor with anti-Ig may be different from the events that induce the same enzyme triggered by SF.", "contents": "Triggering mechanism of B lymphocytes. II. Induction of ornithine decarboxylase in B cells by anti-immunoglobulin and enhancing soluble factor. In vitro stimulation of rabbit lymphocytes with anti-immunoglobulin antibody (anti-Ig) induced ornithine decarboxylase (ODC) (EC4.1.1.17) in the cells. The enzyme activity reached the maximum at 8 hr after stimulation of the cell with antibody and returned to the unstimulated level after 20 hr. The optimum concentration of antibody to produce the maximum activity of ODC was 25 mug/ml, which was in the same range that required for the maximum production of IgG and the highest rate of DNA synthesis. ODC was again induced with soluble factor (SF) in the cells that had been previously treated with anti-Ig antibody for 24 hr. The treatment of the cells with anti-thymocyte serum and complement did not affect the activity of the enzyme induced by anti-Ig or by SF, indicating that enzyme induction was an event occurring in B cells and not in T cells. The addition of mitotic inhibitors, such as hydroxy-urea (HU) or cytosine arabinoside (Ara-C), to the culture with anti-Ig did not inhibit the production of ODC. The presence of cytochalasin-B with anti-Ig did not inhibit, but rather enhanced, the synthesis of ODC. On the other hand, the presence of HU or cytochalasin-B with soluble factor completely suppressed the induction ODC. These results suggested that intracellular events that trigger the induction of ODC by the interaction of Ig receptor with anti-Ig may be different from the events that induce the same enzyme triggered by SF."}
{"id": "PMID:1080775", "title": "B lymphocyte differentiation from fetal liver stem cells in89 Sr-treated mice1.", "content": "89Sr treatment was used to determine if stem cells in young fetal liver could differentiate to mature B lymphocytes in adult animals that lacked functional bone marrow. Isotope injection alone resulted in a severe and lasting depletion of bone marrow cells and particularly lymphocytes, multipotential stem cells, and progenitors for granulocytes and macrophages. The latter two functional populations of cells expanded in number in the spleen but not the liver after their destruction in bone marrow. The total body content of stem cells did not, however, return to normal. Spleen B cells and lymphocytes lacking B or T cell markers were reduced by 89Sr treatment and possible reasons for this depletion are discussed. B lymphocytes were formed in lethally x-irradiated mice that had less than 1% of normal bone marrow function after reconstitution with fetal liver cells. These cells were immunologically mature by virtue of their ability to mount a primary immune response and function in a thymus-independent adoptive anti-hapten response.", "contents": "B lymphocyte differentiation from fetal liver stem cells in89 Sr-treated mice1. 89Sr treatment was used to determine if stem cells in young fetal liver could differentiate to mature B lymphocytes in adult animals that lacked functional bone marrow. Isotope injection alone resulted in a severe and lasting depletion of bone marrow cells and particularly lymphocytes, multipotential stem cells, and progenitors for granulocytes and macrophages. The latter two functional populations of cells expanded in number in the spleen but not the liver after their destruction in bone marrow. The total body content of stem cells did not, however, return to normal. Spleen B cells and lymphocytes lacking B or T cell markers were reduced by 89Sr treatment and possible reasons for this depletion are discussed. B lymphocytes were formed in lethally x-irradiated mice that had less than 1% of normal bone marrow function after reconstitution with fetal liver cells. These cells were immunologically mature by virtue of their ability to mount a primary immune response and function in a thymus-independent adoptive anti-hapten response."}
{"id": "PMID:1080776", "title": "Immune response to a syngeneic mammary adenocarcinoma. I. Comparison of kinetics of tumor cell growth and cytotoxic responses in syngeneic and allogeneic rats.", "content": "The rat mammary adenocarcinoma 13762A is weakly immunogenic in syngeneic hosts. Transplantation immunity is not developed after injection of irradiated tumor cells. Two cell types were isolated from the tumor: one grew slowly forming solid implants; the second grew rapidly in ascites form, even in allogeneic hosts. Spleen cells cytotoxic for a tissue culture derivative of the tumor were produced in animals after injection of either cell type indicating a common antigen. The kinetics of tumor growth and production of cytotoxic spleen cells were compared in syngeneic and allogeneic animals. In vivo rejection of the tumor in allogeneic hosts did not correlate with the in vitro assay of spleen lymphocyte cytotoxicity. Futhermore, the cytotoxic response of spleen cells from syngeneic tumor-bearing hosts parallels that found in allogeneic hosts.", "contents": "Immune response to a syngeneic mammary adenocarcinoma. I. Comparison of kinetics of tumor cell growth and cytotoxic responses in syngeneic and allogeneic rats. The rat mammary adenocarcinoma 13762A is weakly immunogenic in syngeneic hosts. Transplantation immunity is not developed after injection of irradiated tumor cells. Two cell types were isolated from the tumor: one grew slowly forming solid implants; the second grew rapidly in ascites form, even in allogeneic hosts. Spleen cells cytotoxic for a tissue culture derivative of the tumor were produced in animals after injection of either cell type indicating a common antigen. The kinetics of tumor growth and production of cytotoxic spleen cells were compared in syngeneic and allogeneic animals. In vivo rejection of the tumor in allogeneic hosts did not correlate with the in vitro assay of spleen lymphocyte cytotoxicity. Futhermore, the cytotoxic response of spleen cells from syngeneic tumor-bearing hosts parallels that found in allogeneic hosts."}
{"id": "PMID:1080777", "title": "IgM antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: the involvement of T and B lymphocytes as effector cells.", "content": "Antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus (MSV) system was analyzed with respect to the subpopulations of effector cells involved in tumor target cell destruction when IgM was used as the sensitizing antibody. With unfractionated sera from animals that had undergone regression primary MSV tumors it was found that macrophages did not contribute to the cytotoxicity induced by normal spleen cells that were syngeneic to the target cells. The IgM fraction of MSV regressor sera was found to induce cytotoxicity against the target cells by immunoadsorbent column-fractionated normal spleen cells, which were either depleted of T cells or B cells, according to the specificity of the columns. Immune IgM was also found to potentiate the activity of MSV regressor spleen cells that had been similarly fractionated. Furthermore, IgM antibody was found to induced cytotoxicity by normal spleen cells which had been depleted of either T or B cells by the appropriate antiserum (anti-T or anti-Ig) in the presence of complement and subsequent recovery of the viable cells by trysinization, filtration, and washing. However, spleen cells treated with both anti-T and anti-Ig sera simultaneously in the presence of complement and subsequet recovery of viable cells, were not induced to be cytotoxic against the IgM-coated tumor target cells. Further support oy T cells was provided by an experiment showing the induction with IgM of cytotoxicity against the target cells by normal thymocytes.", "contents": "IgM antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: the involvement of T and B lymphocytes as effector cells. Antibody-dependent cell-mediated cytotoxicity in the Moloney sarcoma virus (MSV) system was analyzed with respect to the subpopulations of effector cells involved in tumor target cell destruction when IgM was used as the sensitizing antibody. With unfractionated sera from animals that had undergone regression primary MSV tumors it was found that macrophages did not contribute to the cytotoxicity induced by normal spleen cells that were syngeneic to the target cells. The IgM fraction of MSV regressor sera was found to induce cytotoxicity against the target cells by immunoadsorbent column-fractionated normal spleen cells, which were either depleted of T cells or B cells, according to the specificity of the columns. Immune IgM was also found to potentiate the activity of MSV regressor spleen cells that had been similarly fractionated. Furthermore, IgM antibody was found to induced cytotoxicity by normal spleen cells which had been depleted of either T or B cells by the appropriate antiserum (anti-T or anti-Ig) in the presence of complement and subsequent recovery of the viable cells by trysinization, filtration, and washing. However, spleen cells treated with both anti-T and anti-Ig sera simultaneously in the presence of complement and subsequet recovery of viable cells, were not induced to be cytotoxic against the IgM-coated tumor target cells. Further support oy T cells was provided by an experiment showing the induction with IgM of cytotoxicity against the target cells by normal thymocytes."}
{"id": "PMID:1080778", "title": "The humoral response of mouse spleen cells to two types of sheep erythrocytes. II. Evidence for gene expression in the B lymphocyte.", "content": "There is multigene control of the response of mouse spleen cells to two types of sheep erytrocytes (H and L). Discriminator strains of mice make a much higher response to extra antigens found only on H SRBC than to the shared antigens found on both types of erythrocyte. Non-discriminator strains respond only to the shared antigens, making a responses as great as the discriminator response to the extra antigens. The cell type in which this genetic control is expressed was investigated by a number of methods for studying the role of the macrophage, T cell, and B cell in the production of anti-SRBC response. The results provide no evidence that the gene(s) operate at the macrophage, T cell, or any other accessory cell level. The data from B cell-limiting dilution experiments are compatible with genetic expression at the B cell level.", "contents": "The humoral response of mouse spleen cells to two types of sheep erythrocytes. II. Evidence for gene expression in the B lymphocyte. There is multigene control of the response of mouse spleen cells to two types of sheep erytrocytes (H and L). Discriminator strains of mice make a much higher response to extra antigens found only on H SRBC than to the shared antigens found on both types of erythrocyte. Non-discriminator strains respond only to the shared antigens, making a responses as great as the discriminator response to the extra antigens. The cell type in which this genetic control is expressed was investigated by a number of methods for studying the role of the macrophage, T cell, and B cell in the production of anti-SRBC response. The results provide no evidence that the gene(s) operate at the macrophage, T cell, or any other accessory cell level. The data from B cell-limiting dilution experiments are compatible with genetic expression at the B cell level."}
{"id": "PMID:1080779", "title": "B cells in the appendix and other lymphoid organs of the rabbit: complement receptor lymphocytes.", "content": "Complement receptor lymphocytes were demonstrated in rabbit lymphoid organs, the spleen containing the highest numbers (35%) and the thymus none. Extremely variable numbers (up to 50%) were detectablein the appendix. B and T cell depletion, rosette depletion, and histologic staining indicate that these CRL are B cells in the rabbit as in other species. The variable behavior of B cells in the appendix suggests a difference between this lymphoid organs population and those of other rabbit lymphoid organs.", "contents": "B cells in the appendix and other lymphoid organs of the rabbit: complement receptor lymphocytes. Complement receptor lymphocytes were demonstrated in rabbit lymphoid organs, the spleen containing the highest numbers (35%) and the thymus none. Extremely variable numbers (up to 50%) were detectablein the appendix. B and T cell depletion, rosette depletion, and histologic staining indicate that these CRL are B cells in the rabbit as in other species. The variable behavior of B cells in the appendix suggests a difference between this lymphoid organs population and those of other rabbit lymphoid organs."}
{"id": "PMID:1080780", "title": "Reappraisal of lymphocyte classification by means of surface morphology.", "content": "Prior scanning electron microscope studies have indicated that T (thymus derived) lymphocytes appear \"smooth\" while human B (thymus independent) lymphocytes appear \"hairy\" with numerous microvilli covering their surfaces. A recent report (24) has raised some doubt as to the validity of classifying lymphocyte subpopulations on this purely morphologic basis. We have examined lymphocytes taken from congenitally athymic \"nude\" mice (lacking T cells) and compared them with those taken from normal littermates and CBA/J mice (both having T cell populations). We have determined that there are no distinguishable differences between the distribution of surface morphologies of lymphocytes from animals either with or without T and B cells. Our studies indicate that the classification of T and B cells, based on cell surface features, requires more than just finding out whether or not the cells are smooth or hairy.", "contents": "Reappraisal of lymphocyte classification by means of surface morphology. Prior scanning electron microscope studies have indicated that T (thymus derived) lymphocytes appear \"smooth\" while human B (thymus independent) lymphocytes appear \"hairy\" with numerous microvilli covering their surfaces. A recent report (24) has raised some doubt as to the validity of classifying lymphocyte subpopulations on this purely morphologic basis. We have examined lymphocytes taken from congenitally athymic \"nude\" mice (lacking T cells) and compared them with those taken from normal littermates and CBA/J mice (both having T cell populations). We have determined that there are no distinguishable differences between the distribution of surface morphologies of lymphocytes from animals either with or without T and B cells. Our studies indicate that the classification of T and B cells, based on cell surface features, requires more than just finding out whether or not the cells are smooth or hairy."}
{"id": "PMID:1080781", "title": "Genetic complementation of histocompatibility-linked Ir genes in the rat.", "content": "Different inbred rat strains that are congenic with respect to the major histocompatibility system (H-1) show either' a low, an intermediate, or a high immune response to the synthetic polypeptide (H, G)-A--L. Two out of four low responder H-1 haplotypes are found to complement intermediate responsiveness to high responsiveness. This effect is observed for the primary and secondary response and is detectable at the humoral and cellular level, i.e., antibody production and anamnestic response in vitro. Interallelic and intergenic complementation models are outlined. The ensuing implications for H-linked Ir gene control of immune responsiveness and for the structure of major histocompatibility systems are discussed.", "contents": "Genetic complementation of histocompatibility-linked Ir genes in the rat. Different inbred rat strains that are congenic with respect to the major histocompatibility system (H-1) show either' a low, an intermediate, or a high immune response to the synthetic polypeptide (H, G)-A--L. Two out of four low responder H-1 haplotypes are found to complement intermediate responsiveness to high responsiveness. This effect is observed for the primary and secondary response and is detectable at the humoral and cellular level, i.e., antibody production and anamnestic response in vitro. Interallelic and intergenic complementation models are outlined. The ensuing implications for H-linked Ir gene control of immune responsiveness and for the structure of major histocompatibility systems are discussed."}
{"id": "PMID:1080782", "title": "Studies of thymic factors. I. Evaluation of the mouse rosette assay for thymic hormone.", "content": "The restoration of sensitivity to azathioprine of athymic (nu/nu) mouse splenic rosette-forming cells (RFC) has been used by others to assay thymic hormone. These previous studies have led to the conclusion that a major factor in the development of autoimmunity in New Zealand (NZ) mice and patients with systemic lupus erythematosus (SLE) is the premature loss of thymic hormone. We have studied the mouse rosette assay and find that poly A-Poly U and single stranded DNA, in addition to thymosin, restored azathioprine sensitivity to nu/nu RFC. Thus, other substances may mimic thymic hormone, perhaps via the cyclic AMP system. Furthermore, antibodies to T cells were found to interfere with the assay. Sera from old NZ mice which contain naturally occurring thymocytotoxic antibodies (NTA) failed to restore azathioprine sensitivity. Small quantities of NTA prevented normal sera from functioning in the assay. We conclude that the role of thymic hormone(s) in the development of autoimmunity in NZ mice and patients with SLE must be completely reevaluated.", "contents": "Studies of thymic factors. I. Evaluation of the mouse rosette assay for thymic hormone. The restoration of sensitivity to azathioprine of athymic (nu/nu) mouse splenic rosette-forming cells (RFC) has been used by others to assay thymic hormone. These previous studies have led to the conclusion that a major factor in the development of autoimmunity in New Zealand (NZ) mice and patients with systemic lupus erythematosus (SLE) is the premature loss of thymic hormone. We have studied the mouse rosette assay and find that poly A-Poly U and single stranded DNA, in addition to thymosin, restored azathioprine sensitivity to nu/nu RFC. Thus, other substances may mimic thymic hormone, perhaps via the cyclic AMP system. Furthermore, antibodies to T cells were found to interfere with the assay. Sera from old NZ mice which contain naturally occurring thymocytotoxic antibodies (NTA) failed to restore azathioprine sensitivity. Small quantities of NTA prevented normal sera from functioning in the assay. We conclude that the role of thymic hormone(s) in the development of autoimmunity in NZ mice and patients with SLE must be completely reevaluated."}
{"id": "PMID:1080784", "title": "T-cell membrane characteristics of \"mycosis cells\" in the skin and lymph node.", "content": "In some patients with mycosis fungoides atypical cells (\"mycosis cells\") are found in the blood. Recently the T-cell membrane characteristics of these atypical cells have been described. In this paper the results of a study of the atypical cells isolated from the lymph nodes and the skin lesions of three patients with mycosis fungoides are presented. Using electron microscopy, it could be demonstrated that the atypical cells formed rosettes with uncoated sheep red blood cells, but not with antibody-complement-coated sheep erythrocytes, indicating the T-cell membrane characteristics of the atypical cells.", "contents": "T-cell membrane characteristics of \"mycosis cells\" in the skin and lymph node. In some patients with mycosis fungoides atypical cells (\"mycosis cells\") are found in the blood. Recently the T-cell membrane characteristics of these atypical cells have been described. In this paper the results of a study of the atypical cells isolated from the lymph nodes and the skin lesions of three patients with mycosis fungoides are presented. Using electron microscopy, it could be demonstrated that the atypical cells formed rosettes with uncoated sheep red blood cells, but not with antibody-complement-coated sheep erythrocytes, indicating the T-cell membrane characteristics of the atypical cells."}
{"id": "PMID:1080786", "title": "Histiocytosis X of the larynx.", "content": "The present report is concerned with the observation of histiocytosis X in the larynx in a man aged 57 years. This localisation of the process is quite unusual and we have found only one similar case in the literature. The differential diagnosis and possible etiological factors are briefly discussed.", "contents": "Histiocytosis X of the larynx. The present report is concerned with the observation of histiocytosis X in the larynx in a man aged 57 years. This localisation of the process is quite unusual and we have found only one similar case in the literature. The differential diagnosis and possible etiological factors are briefly discussed."}
{"id": "PMID:1080788", "title": "X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanisms underlying the immune defect.", "content": "The mechanisms underlying the X-linked thymus-independent (B) lymphocyte functional defect in the CBA/N (CN) mice and their F1 progeny were studied. Immune defective mice were unable to respond to the T-independent antigen 2,4-dinitrophenyl-lysyl-derivative of Ficoll (DNP-lys-Ficoll) but were able to form antibody against the highly cross-reactive hapten (trinitrophenyl) when it was coupled to an erythrocyte carrier. Immune defective CN X DBA/2N (DN) F1 male mice, which do not normally respond to T-independent antigens, were able to respond to both polyribosinic-polyribocytidylic acid and DNP-lys-Ficoll after the administration of CN X DN F1 female spleen cells even if these cells had been depleted of T lymphocytes. In addition, it was shown that the inability of the CN mice and their F1 progeny to respond to T-independent antigens was not due to an intrinsic abnormality of their microenvironment or the suppressive actions of a T lymphocyte. Our data present evidence that the X-linked defect in the CN mice is due to an intrinsic defect in B-lymphocyte development.", "contents": "X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanisms underlying the immune defect. The mechanisms underlying the X-linked thymus-independent (B) lymphocyte functional defect in the CBA/N (CN) mice and their F1 progeny were studied. Immune defective mice were unable to respond to the T-independent antigen 2,4-dinitrophenyl-lysyl-derivative of Ficoll (DNP-lys-Ficoll) but were able to form antibody against the highly cross-reactive hapten (trinitrophenyl) when it was coupled to an erythrocyte carrier. Immune defective CN X DBA/2N (DN) F1 male mice, which do not normally respond to T-independent antigens, were able to respond to both polyribosinic-polyribocytidylic acid and DNP-lys-Ficoll after the administration of CN X DN F1 female spleen cells even if these cells had been depleted of T lymphocytes. In addition, it was shown that the inability of the CN mice and their F1 progeny to respond to T-independent antigens was not due to an intrinsic abnormality of their microenvironment or the suppressive actions of a T lymphocyte. Our data present evidence that the X-linked defect in the CN mice is due to an intrinsic defect in B-lymphocyte development."}
{"id": "PMID:1080789", "title": "The ontogeny of antigen-specific T cells.", "content": "Although the fetal lamb is unable to form circulating antiovalbumin antibodies until about 120 days of gestation, ovalbumin-specific helper T cells can be stimulated to function at an earlier age. This would suggest that the critical event responsible for the precise sequential maturation of immunologic competence to different antigens at different developmental stages is not the first appearance of specific receptors on immunocytes. Alternative explanations of this phenomenon are discussed.", "contents": "The ontogeny of antigen-specific T cells. Although the fetal lamb is unable to form circulating antiovalbumin antibodies until about 120 days of gestation, ovalbumin-specific helper T cells can be stimulated to function at an earlier age. This would suggest that the critical event responsible for the precise sequential maturation of immunologic competence to different antigens at different developmental stages is not the first appearance of specific receptors on immunocytes. Alternative explanations of this phenomenon are discussed."}
{"id": "PMID:1080790", "title": "Localization of aggregated cell surface antigens of target cells bound to cytotoxic T lymphocytes.", "content": "The redistribution of aggregated cell surface antigens of target cells bound to cytotoxic T lymphocytes was investigated. It was found that cap formation induced by antibody always occurred toward the site of binding. It is suggested that the polar localization of capped target cell surface determinants is a consequence of cytotoxic T-lymphocyte target cell interaction.", "contents": "Localization of aggregated cell surface antigens of target cells bound to cytotoxic T lymphocytes. The redistribution of aggregated cell surface antigens of target cells bound to cytotoxic T lymphocytes was investigated. It was found that cap formation induced by antibody always occurred toward the site of binding. It is suggested that the polar localization of capped target cell surface determinants is a consequence of cytotoxic T-lymphocyte target cell interaction."}
{"id": "PMID:1080791", "title": "Kinetic study of T lymphocytes after sensitization against soluble antigen. III. Potentiation and suppression of the PHA response by antigen-activated lymphocytes of low density.", "content": "Lymph node cells of ovalbumin-sensitized rats were separated on the basis of buoyant density into fractions reciprocally enriched in cells responsive to ovalbumin or phytohemagglutinin (PHA). Recombination of high density and low density fractions in varying proportions resulted in potentiation or suppression of the DNA synthetic response to PHA in culture. The response of cultures containing equal numbers of high and low density cells was markedly greater than the sum of the two populations stimulated separately. However, when decreasing numbers of low density cells were cultured with a constant number of high density cells, profound suppression was observed.", "contents": "Kinetic study of T lymphocytes after sensitization against soluble antigen. III. Potentiation and suppression of the PHA response by antigen-activated lymphocytes of low density. Lymph node cells of ovalbumin-sensitized rats were separated on the basis of buoyant density into fractions reciprocally enriched in cells responsive to ovalbumin or phytohemagglutinin (PHA). Recombination of high density and low density fractions in varying proportions resulted in potentiation or suppression of the DNA synthetic response to PHA in culture. The response of cultures containing equal numbers of high and low density cells was markedly greater than the sum of the two populations stimulated separately. However, when decreasing numbers of low density cells were cultured with a constant number of high density cells, profound suppression was observed."}
{"id": "PMID:1080792", "title": "Clonal growth of hamster free alveolar cells in soft agar.", "content": "Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes were found in the colony. Medium conditioned by baby hamster kidney cells or other cells was required for the initiation and maintenance of their growth. Alveolar cells from normal mice and rats also formed colonies under appropriate culture conditions.", "contents": "Clonal growth of hamster free alveolar cells in soft agar. Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes were found in the colony. Medium conditioned by baby hamster kidney cells or other cells was required for the initiation and maintenance of their growth. Alveolar cells from normal mice and rats also formed colonies under appropriate culture conditions."}
{"id": "PMID:1080793", "title": "Coelomic fluid and blood serum proteins of the metamorphosing bullfrog tadpole.", "content": "Bullfrog (Rana catesbeiana) tadpoles were divided into four groups containing Gosner ('60) stages as follows: I, 27-31; II, 32-36; III, 37-41; IV, 42-46. Extractable coelomic fluid volume from late metamorphic tadpoles (Group IV) was found to have a highly significant (p less than 0.001) negative correlation with stage; tadpole weight also had a negative correlation with stage (p less than 0.025). No significant correlation was found between coelomic fluid volume and tadpole weight. Group IV blood serum protein levels (x = 1.30 +/- SEM 0.10 G/100 ml serum) were shown to be significantly higher than corresponding coelomic fluid serum protein levels (x = 0.80 +/- 0.06 G/100 ml). Electrophoresis data demonstrated an increase in the albumin fraction of both blood serum and coelomic fluid during metamorphosis. I-125 labeled human serum albumin equilibrated with the blood serum at equal concentrations about ten hours after an intracoelomic injection. A coelomic fluid turnover time fo 26% per hour was calculated from the tracer data. We hypothesize that a slight sieving occurs at the coelomic fluid inflow site with none at the outflow site.", "contents": "Coelomic fluid and blood serum proteins of the metamorphosing bullfrog tadpole. Bullfrog (Rana catesbeiana) tadpoles were divided into four groups containing Gosner ('60) stages as follows: I, 27-31; II, 32-36; III, 37-41; IV, 42-46. Extractable coelomic fluid volume from late metamorphic tadpoles (Group IV) was found to have a highly significant (p less than 0.001) negative correlation with stage; tadpole weight also had a negative correlation with stage (p less than 0.025). No significant correlation was found between coelomic fluid volume and tadpole weight. Group IV blood serum protein levels (x = 1.30 +/- SEM 0.10 G/100 ml serum) were shown to be significantly higher than corresponding coelomic fluid serum protein levels (x = 0.80 +/- 0.06 G/100 ml). Electrophoresis data demonstrated an increase in the albumin fraction of both blood serum and coelomic fluid during metamorphosis. I-125 labeled human serum albumin equilibrated with the blood serum at equal concentrations about ten hours after an intracoelomic injection. A coelomic fluid turnover time fo 26% per hour was calculated from the tracer data. We hypothesize that a slight sieving occurs at the coelomic fluid inflow site with none at the outflow site."}
{"id": "PMID:1080794", "title": "Fertilization of green frog (Rana clamitans) eggs in their native jelly by bullfrog (Rana catesbeiana) sperm.", "content": "Fertilization of R. clamitans eggs in their native jelly occurred when inseminated with high concentrations of R. catesbeiana sperm. Contrary to previous evidence, the block to cross-fertilization of the R. clamitans egg is not complete. This raises the possibility that R. clamitans x R. catesbeiana hybrids may form in nature.", "contents": "Fertilization of green frog (Rana clamitans) eggs in their native jelly by bullfrog (Rana catesbeiana) sperm. Fertilization of R. clamitans eggs in their native jelly occurred when inseminated with high concentrations of R. catesbeiana sperm. Contrary to previous evidence, the block to cross-fertilization of the R. clamitans egg is not complete. This raises the possibility that R. clamitans x R. catesbeiana hybrids may form in nature."}
{"id": "PMID:1080795", "title": "Structural dynamics of frog muscle during isometric contraction.", "content": "Intensity fluctuation autocorrelation functions of laser light scattered by actively contracting muscle were measured at points in the scattered field. They were reproducible and showed characteristics which depended on the physiological state of the muscle and the parameters of the scattering geometry. The autocorrelation functions had large amplitudes and decay rates that varied significantly with the phase of the contraction-relaxation cycle. The dependence of the autocorrelation function on scattering geometry indicated many elements with diameters on the order of 0.5 mum (presumed to be myofibrillar sarcomeres or their A bands or I bands) undergo independent random changes in their axial positions and their internal distribution of optical polarizability during the plateau of an isometric tetanus. The experimental results are interpreted in terms of a model in which most of the scattering elements in isometrically contracting muscle have random fluctuating axial velocities of average magnitude 20 nm/ms that persist for a few milliseconds at least. In addition to these axial motions there are local fluctuations in polarizability. Similar intensity fluctuation autocorrelation functions were observed throughout the active state on two muscle preparations, whole sartorius muscle and small bundles of single fibers (three to eight) of semitendinosus muscle. These results imply that the tension developed during an isometric tetanus contains a fluctuating component as well as a constant component.", "contents": "Structural dynamics of frog muscle during isometric contraction. Intensity fluctuation autocorrelation functions of laser light scattered by actively contracting muscle were measured at points in the scattered field. They were reproducible and showed characteristics which depended on the physiological state of the muscle and the parameters of the scattering geometry. The autocorrelation functions had large amplitudes and decay rates that varied significantly with the phase of the contraction-relaxation cycle. The dependence of the autocorrelation function on scattering geometry indicated many elements with diameters on the order of 0.5 mum (presumed to be myofibrillar sarcomeres or their A bands or I bands) undergo independent random changes in their axial positions and their internal distribution of optical polarizability during the plateau of an isometric tetanus. The experimental results are interpreted in terms of a model in which most of the scattering elements in isometrically contracting muscle have random fluctuating axial velocities of average magnitude 20 nm/ms that persist for a few milliseconds at least. In addition to these axial motions there are local fluctuations in polarizability. Similar intensity fluctuation autocorrelation functions were observed throughout the active state on two muscle preparations, whole sartorius muscle and small bundles of single fibers (three to eight) of semitendinosus muscle. These results imply that the tension developed during an isometric tetanus contains a fluctuating component as well as a constant component."}
{"id": "PMID:1080796", "title": "Actions of external hypertonic urea, ADH, and theophylline on transcellular and extracellular solute permeabilities in frog skin.", "content": "Increases in transepithelial solute permeability were elicited in the frog skin with external hypertonic urea, theophylline, and vasopressin (ADH). In external hypertonic urea, which is known to increase the permeability of the extracellular (paracellular) pathway, the unidirectional transepithelial fluxes of Na (passive), K, Cl, and urea increased substantially while preserving a linear relationship to each other. The same linear relationship was also observed for the passive Na and urea fluxes in regular Ringer and under stimulation with ADH or 10 mM theophylline, indicating that their permeation pathway was extracellular. A linear relationship between Cl and urea fluxes could be demonstrated if the skins were separated according to their open circuit potentials; parallel lines were obtained with increasing intercepts on the Cl axis as the open circuit potential decreased. The slopes of the Cl vs. urea lines were not different from that obtained in external hypertonic urea, indicating that this relationship described the extracellular movement of Cl. The intercept on the ordinate was interpreted as the contribution from the transcellular Cl movement. In the presence of 0.5 mM theophylline or 10 mU/ml of ADH, mainly the transcellular movement of Cl increased, whereas 10 mM theophylline caused increases in both transcellular and extracellular Cl fluxes. These and other data were interpreted in terms of a possible intracellular control of the theophylline-induced increase in extracellular fluxes. The changes in passive solute permeability were shown to be independent of active transport. The responses of the active transport system, the transcellular and paracellular pathways to theophylline and ADH could be explained in terms of the different resulting concentrations of cyclic 3'-5'-AMP produced by each of these substances in the tissue.", "contents": "Actions of external hypertonic urea, ADH, and theophylline on transcellular and extracellular solute permeabilities in frog skin. Increases in transepithelial solute permeability were elicited in the frog skin with external hypertonic urea, theophylline, and vasopressin (ADH). In external hypertonic urea, which is known to increase the permeability of the extracellular (paracellular) pathway, the unidirectional transepithelial fluxes of Na (passive), K, Cl, and urea increased substantially while preserving a linear relationship to each other. The same linear relationship was also observed for the passive Na and urea fluxes in regular Ringer and under stimulation with ADH or 10 mM theophylline, indicating that their permeation pathway was extracellular. A linear relationship between Cl and urea fluxes could be demonstrated if the skins were separated according to their open circuit potentials; parallel lines were obtained with increasing intercepts on the Cl axis as the open circuit potential decreased. The slopes of the Cl vs. urea lines were not different from that obtained in external hypertonic urea, indicating that this relationship described the extracellular movement of Cl. The intercept on the ordinate was interpreted as the contribution from the transcellular Cl movement. In the presence of 0.5 mM theophylline or 10 mU/ml of ADH, mainly the transcellular movement of Cl increased, whereas 10 mM theophylline caused increases in both transcellular and extracellular Cl fluxes. These and other data were interpreted in terms of a possible intracellular control of the theophylline-induced increase in extracellular fluxes. The changes in passive solute permeability were shown to be independent of active transport. The responses of the active transport system, the transcellular and paracellular pathways to theophylline and ADH could be explained in terms of the different resulting concentrations of cyclic 3'-5'-AMP produced by each of these substances in the tissue."}
{"id": "PMID:1080797", "title": "Resolution of the potassium ion pump in muscle fibers using barium ions.", "content": "When frog sartorius muscles recover from Na enrichment in the presence of external K, net K entry into the fibers occurs by both passive movement and active inward transport via a K pump. Under normal conditions, it has not been possible to experimentally distinguish these processes. Fractionation into the flux components must be accomplished from inferences concerning the K conductance or permeability during a period of rapid Na extrusion. The best estimates indicate that 60-80% of the K entry occurs via the K pump. In the presence of Ba ions, the membrane permeability to K is very much reduced. Under these conditions, Na-enriched muscles underwent a normal recovery in the presence of external K, and the amount of inward K movement due to the K pump rose to over 90% of the total K entry. The characteristics of the K pump studied by this means were: (a) essentially complete inhibition by 10(-4) M ouabain, (b) inhibition by [Na]omicron, (c) activation by [K]omicron according to a rectangular hyperbola in the absence of [Na]omicron, (d) linear activation by [Na]iota over a wide range in concentration, (e) zero or undetectably low pumping rate as [Na]iota leads to 0, (f) the number of Na ions actively transported per K ion actively transported is 1.4-1.7 normally and 1.1 in the presence of Ba.", "contents": "Resolution of the potassium ion pump in muscle fibers using barium ions. When frog sartorius muscles recover from Na enrichment in the presence of external K, net K entry into the fibers occurs by both passive movement and active inward transport via a K pump. Under normal conditions, it has not been possible to experimentally distinguish these processes. Fractionation into the flux components must be accomplished from inferences concerning the K conductance or permeability during a period of rapid Na extrusion. The best estimates indicate that 60-80% of the K entry occurs via the K pump. In the presence of Ba ions, the membrane permeability to K is very much reduced. Under these conditions, Na-enriched muscles underwent a normal recovery in the presence of external K, and the amount of inward K movement due to the K pump rose to over 90% of the total K entry. The characteristics of the K pump studied by this means were: (a) essentially complete inhibition by 10(-4) M ouabain, (b) inhibition by [Na]omicron, (c) activation by [K]omicron according to a rectangular hyperbola in the absence of [Na]omicron, (d) linear activation by [Na]iota over a wide range in concentration, (e) zero or undetectably low pumping rate as [Na]iota leads to 0, (f) the number of Na ions actively transported per K ion actively transported is 1.4-1.7 normally and 1.1 in the presence of Ba."}
{"id": "PMID:1080798", "title": "Variation of muscle stiffness with force at increasing speeds of shortening.", "content": "Single frog skeletal muscle fibers were attached to a servo motor and force transducer by knotting the tendons to pieces of wire at the fiver insertions. Small amplitude, high frequency sinusoidal length changes were then applied during tetani while fibers contracted both isometrically and isotonically at various constant velocities. The amlitude of the resulting force oscillation provides a relative measure of muscle stiffness. It is shown from an analysis of the transient force responses observed after sudden changes in muscle length applied both at full and reduced overlap and during the rising phase of short tetani that these responses can be explained on the basis of varying numbers of cross bridges attached at the time of the length step. Therefore, the stiffness measured by the high frequency legth oscillation method is taken to be directly proportional to the number of cross bridges attached to thin filament sittes. It is found that muscle stiffness measured in this way falls with increasing shortening velocity, but not as rapidly as the force. The results suggest that at the maximum velocity of shortening, when the external force is zero, muscle stiffness is still substantial. The findings are interpreted in terms of a specific model for muscle contraction in which the maximum velocity of shortening under zero external load arises when a force balance is attained between attached cross bridges somr interpretations of these results are also discussed.", "contents": "Variation of muscle stiffness with force at increasing speeds of shortening. Single frog skeletal muscle fibers were attached to a servo motor and force transducer by knotting the tendons to pieces of wire at the fiver insertions. Small amplitude, high frequency sinusoidal length changes were then applied during tetani while fibers contracted both isometrically and isotonically at various constant velocities. The amlitude of the resulting force oscillation provides a relative measure of muscle stiffness. It is shown from an analysis of the transient force responses observed after sudden changes in muscle length applied both at full and reduced overlap and during the rising phase of short tetani that these responses can be explained on the basis of varying numbers of cross bridges attached at the time of the length step. Therefore, the stiffness measured by the high frequency legth oscillation method is taken to be directly proportional to the number of cross bridges attached to thin filament sittes. It is found that muscle stiffness measured in this way falls with increasing shortening velocity, but not as rapidly as the force. The results suggest that at the maximum velocity of shortening, when the external force is zero, muscle stiffness is still substantial. The findings are interpreted in terms of a specific model for muscle contraction in which the maximum velocity of shortening under zero external load arises when a force balance is attained between attached cross bridges somr interpretations of these results are also discussed."}
{"id": "PMID:1080799", "title": "Attempts to measure endogenous levels of dopa and 5-hydroxytryptophan in rat brain.", "content": "We have used previously published analytical methods of high sensitivity, developed in our laboratory, in an attempt to detect endogenous dopa and 5-hydroxytryptophan (5-HTP) in normal rat brain. In the brains of rats killed by decapitation we were unable to detect any dopa and found only a few ng/g of 5-HTP. Higher levels were found in the brains of rats killed by exposing their heads to microwaves. The levels of dopa found under these conditions varied considerably between different rat strains, and also between different experiments on the same strain, ranging between 2.5 and 13.8 ng/g. True resting levels may be lower than those observed because the procedure necessitated a short-lasting immobilization stress which increased the dopa (but not 5-HTP) levels. The 5-HTP levels were also variable and ranged between 5 and 25 ng/g. The highest levels of dopa and 5-HTP were found in the Japanese Wistar strain with spontaneous hypertension. Some analytical pitfalls are pointed out.", "contents": "Attempts to measure endogenous levels of dopa and 5-hydroxytryptophan in rat brain. We have used previously published analytical methods of high sensitivity, developed in our laboratory, in an attempt to detect endogenous dopa and 5-hydroxytryptophan (5-HTP) in normal rat brain. In the brains of rats killed by decapitation we were unable to detect any dopa and found only a few ng/g of 5-HTP. Higher levels were found in the brains of rats killed by exposing their heads to microwaves. The levels of dopa found under these conditions varied considerably between different rat strains, and also between different experiments on the same strain, ranging between 2.5 and 13.8 ng/g. True resting levels may be lower than those observed because the procedure necessitated a short-lasting immobilization stress which increased the dopa (but not 5-HTP) levels. The 5-HTP levels were also variable and ranged between 5 and 25 ng/g. The highest levels of dopa and 5-HTP were found in the Japanese Wistar strain with spontaneous hypertension. Some analytical pitfalls are pointed out."}
{"id": "PMID:1080800", "title": "[Pineal organ and parietal eye of Lacerta vivipara: a qualitative and quantitative microscopic study of the uptake of 3H-5-hydroxytryptophan during ontogenesis].", "content": "After comparison of results obtained by different modalities of 3h-5HTP injections (involving intraallantodic, intraventricular and intraperitoneal injections), the intraperitoneal one was retaineed to study experimental cytochemcal biosynthesis of indoleamines, in the epiphysis of Lacerta vivpara J., during ontogenesis. From stage 36 of embryogenesis, the selective radioautographic labelling, significant of atritiated indoleamines storage, was located in the distal part of the epiphysis. From stage 36 to 40 (just before hatching) and 3 days after hatching, microscopic and microphotometric studies point out, in the epiphysis epithelium, a highly significant increase in tritiated infoleamines rate. In contrast, the weak nonspecific reactions, in the neighbouring brain structures (dorsal telencephalon, tectum opticum and paraphysis), stay constant. The metabolism and storage sites of indoleamines belong to sensory line cells. During ontogenesis, these sites appear successively in distal, middle and proximal epiphysis and from the basal to the upper part of the sensory line cells. The embryonic parietal eye, in spite of structural analogies, does not take up the radioactive compound.", "contents": "[Pineal organ and parietal eye of Lacerta vivipara: a qualitative and quantitative microscopic study of the uptake of 3H-5-hydroxytryptophan during ontogenesis]. After comparison of results obtained by different modalities of 3h-5HTP injections (involving intraallantodic, intraventricular and intraperitoneal injections), the intraperitoneal one was retaineed to study experimental cytochemcal biosynthesis of indoleamines, in the epiphysis of Lacerta vivpara J., during ontogenesis. From stage 36 of embryogenesis, the selective radioautographic labelling, significant of atritiated indoleamines storage, was located in the distal part of the epiphysis. From stage 36 to 40 (just before hatching) and 3 days after hatching, microscopic and microphotometric studies point out, in the epiphysis epithelium, a highly significant increase in tritiated infoleamines rate. In contrast, the weak nonspecific reactions, in the neighbouring brain structures (dorsal telencephalon, tectum opticum and paraphysis), stay constant. The metabolism and storage sites of indoleamines belong to sensory line cells. During ontogenesis, these sites appear successively in distal, middle and proximal epiphysis and from the basal to the upper part of the sensory line cells. The embryonic parietal eye, in spite of structural analogies, does not take up the radioactive compound."}
{"id": "PMID:1080802", "title": "The life cycle of Haematoloechus coloradensis Cort 1915 (Digenea: Plagiorchiidae), with emphasis on host susceptibility to infection.", "content": "Mother and daughter sporocysts and xiphidiocercariae of Haematoloechus coloradensis develop in the snail, Physa virgata. Cercariae penerate and encyst in dragonfly nymphs, Tramea sp., Libellula sp., Anax sp., and in damselfly nymphs, Enallagma spp. Upon ingestion by the vertebrate host, metacercariae excyst and migrate into the lungs where they mature. Haematoloechus coloradensis is specific for its definitive host, R. pipiens, but shows some development in Ambystoma tigrinum. Although H. coloradensis is specific for its snail host, all odonate species tested served equally well as the second intermediate host.", "contents": "The life cycle of Haematoloechus coloradensis Cort 1915 (Digenea: Plagiorchiidae), with emphasis on host susceptibility to infection. Mother and daughter sporocysts and xiphidiocercariae of Haematoloechus coloradensis develop in the snail, Physa virgata. Cercariae penerate and encyst in dragonfly nymphs, Tramea sp., Libellula sp., Anax sp., and in damselfly nymphs, Enallagma spp. Upon ingestion by the vertebrate host, metacercariae excyst and migrate into the lungs where they mature. Haematoloechus coloradensis is specific for its definitive host, R. pipiens, but shows some development in Ambystoma tigrinum. Although H. coloradensis is specific for its snail host, all odonate species tested served equally well as the second intermediate host."}
{"id": "PMID:1080803", "title": "The effect of temperature on desensitization kinetics at the post-synaptic membrane of the frog muscle fibre.", "content": "1. The time course of acetylcholine (ACh) potentials during development of desensitization was prolonged when a double-barrel ACh pipette was used for evoking desensitization. When a single-barrel ACh pipette was used, no change in the time course of ACh potential was to be seen during desensitization. 2. The recovery after desensitization did not depend on the rate of onset or on its level when a single ACh pipette was used. The half-time of recovery had a constant value of about 5.8 sec in the presence of chlorpromazine or SKF-525 A in a muscle bath at 22 degrees C. 3. Unlike the rate of onset, recovery from desensitization does not depend on the membrane potential. 4. The rate of onset of desensitization, i.e. time taken for reduction of ACh potentials to half-way between the initial amplitude and final steady value, decreased when temperature of the muscle bath was lowered. 5. Q10 of desensitization onset was found to be 1.5 for a change of temperature from 32 to 22 degrees C, 1.9 from 22 to 12 degrees C, 2.6 from 12 to 5 degrees C and 3.3 from 12 to 2 degrees C. 6. A similar temperature effect was observed in the case of desensitization recovery, the Q10 being 1.2 for temperature changes from 32 to 22 degrees C, 1.3 from 22 to 12 degrees C and 2.36 from 12 to 2 degrees C. 7. Intracellular application of quaternary methiodide of SKF-525 A or chloropromazine caused more rapid desensitization by ACh. The rate of desensitization onset depends on the ACh dose and on the frequency of application. The rate of recovery, however, has a constant value with a half-time of 5.5-5.7 sec at 22 degrees C. 8. Both the rate of onset and the rate of recovery changed with temperature in the case of intracellular potentiation of desensitization, in a similar manner to that observed after extracellular application of these drugs. 9. The onset of desensitization can thus be influenced by different substances as well as by changes in temperature. Recovery apparently has a different mechanism from the onset, because its time course can be altered only by changes in temperature of the muscle.", "contents": "The effect of temperature on desensitization kinetics at the post-synaptic membrane of the frog muscle fibre. 1. The time course of acetylcholine (ACh) potentials during development of desensitization was prolonged when a double-barrel ACh pipette was used for evoking desensitization. When a single-barrel ACh pipette was used, no change in the time course of ACh potential was to be seen during desensitization. 2. The recovery after desensitization did not depend on the rate of onset or on its level when a single ACh pipette was used. The half-time of recovery had a constant value of about 5.8 sec in the presence of chlorpromazine or SKF-525 A in a muscle bath at 22 degrees C. 3. Unlike the rate of onset, recovery from desensitization does not depend on the membrane potential. 4. The rate of onset of desensitization, i.e. time taken for reduction of ACh potentials to half-way between the initial amplitude and final steady value, decreased when temperature of the muscle bath was lowered. 5. Q10 of desensitization onset was found to be 1.5 for a change of temperature from 32 to 22 degrees C, 1.9 from 22 to 12 degrees C, 2.6 from 12 to 5 degrees C and 3.3 from 12 to 2 degrees C. 6. A similar temperature effect was observed in the case of desensitization recovery, the Q10 being 1.2 for temperature changes from 32 to 22 degrees C, 1.3 from 22 to 12 degrees C and 2.36 from 12 to 2 degrees C. 7. Intracellular application of quaternary methiodide of SKF-525 A or chloropromazine caused more rapid desensitization by ACh. The rate of desensitization onset depends on the ACh dose and on the frequency of application. The rate of recovery, however, has a constant value with a half-time of 5.5-5.7 sec at 22 degrees C. 8. Both the rate of onset and the rate of recovery changed with temperature in the case of intracellular potentiation of desensitization, in a similar manner to that observed after extracellular application of these drugs. 9. The onset of desensitization can thus be influenced by different substances as well as by changes in temperature. Recovery apparently has a different mechanism from the onset, because its time course can be altered only by changes in temperature of the muscle."}
{"id": "PMID:1080804", "title": "Stimulation by aldosterone of the sodium efflux in barnacle muscle fibres: effects of RNA inhibitors and spironolactone.", "content": "1. Single muscle fibres from the barnacle Balanus nubilus have been studied to provide information about the mode of action of aldosterone on Na transport in a symmetric cell. 2. Neither internal nor external application of aldosterone modified the course of the Na efflux. However, fibres pre-exposed overnight to a dose of aldosterone showed a biphasic response to external aldosterone. The first phase was prompt in onset and consisted of a falling rate constant for Na efflux becoming a constant. This has been interpreted as indicating that aldosterone acts by releasing sequestered or bound Na. The second phase was delayed in onset with the average latent period being 68 min. It consisted of a step-up in the rate of Na efflux, followed by a transitory rise in the efflux rate constant. This second phase was dose-dependent, the minimum concentration being 10(-9) M. 3. Internal application of aldosterone in a concentration as low as 10(-10) M promptly stopped the rate constant for Na efflux from further falling but failed to cause delayed stimulation. 4. The response of the Na efflux into Li-ASW following external application of aldosterone was bi-phasic. The magnitude of the delayed stimulation was comparable to that found in controls. 5. No correlation between latent period or size of the internal Na bound fraction, and the magnitude of the delayed stimulation could be established. 6. Internal application of actinomycin-D, alpha-amanitin or cordycepin shortly before application of external aldosterone caused complete abolition of the delayed stimulation. 7. Overnight pre-exposure of the barnacle to actinomycin-D caused complete abolition of the falling rate constant for Na efflux, as well as the delayed stimulation caused by external aldosterone. 8. Internal application of spironolactone SC-14266 shortly before external application of aldosterone caused complete abolition of the biphasic response to the steroid. 9. It is concluded that (i) barnacle fibres can be made sensitive to aldosterone, (ii) the biphasic effects on the Na efflux depend on prior induction of RNA, (iii) the first effect caused by internal or external aldosterone involves mobilization of sequestered Na, (iv) the results obtained with spironolactone are in keeping with the current view that aldosterone interacts with a receptor system before causing de-repression. 10. The implications of the finding that aldosterone releases sequestered Na are briefly touched upon.", "contents": "Stimulation by aldosterone of the sodium efflux in barnacle muscle fibres: effects of RNA inhibitors and spironolactone. 1. Single muscle fibres from the barnacle Balanus nubilus have been studied to provide information about the mode of action of aldosterone on Na transport in a symmetric cell. 2. Neither internal nor external application of aldosterone modified the course of the Na efflux. However, fibres pre-exposed overnight to a dose of aldosterone showed a biphasic response to external aldosterone. The first phase was prompt in onset and consisted of a falling rate constant for Na efflux becoming a constant. This has been interpreted as indicating that aldosterone acts by releasing sequestered or bound Na. The second phase was delayed in onset with the average latent period being 68 min. It consisted of a step-up in the rate of Na efflux, followed by a transitory rise in the efflux rate constant. This second phase was dose-dependent, the minimum concentration being 10(-9) M. 3. Internal application of aldosterone in a concentration as low as 10(-10) M promptly stopped the rate constant for Na efflux from further falling but failed to cause delayed stimulation. 4. The response of the Na efflux into Li-ASW following external application of aldosterone was bi-phasic. The magnitude of the delayed stimulation was comparable to that found in controls. 5. No correlation between latent period or size of the internal Na bound fraction, and the magnitude of the delayed stimulation could be established. 6. Internal application of actinomycin-D, alpha-amanitin or cordycepin shortly before application of external aldosterone caused complete abolition of the delayed stimulation. 7. Overnight pre-exposure of the barnacle to actinomycin-D caused complete abolition of the falling rate constant for Na efflux, as well as the delayed stimulation caused by external aldosterone. 8. Internal application of spironolactone SC-14266 shortly before external application of aldosterone caused complete abolition of the biphasic response to the steroid. 9. It is concluded that (i) barnacle fibres can be made sensitive to aldosterone, (ii) the biphasic effects on the Na efflux depend on prior induction of RNA, (iii) the first effect caused by internal or external aldosterone involves mobilization of sequestered Na, (iv) the results obtained with spironolactone are in keeping with the current view that aldosterone interacts with a receptor system before causing de-repression. 10. The implications of the finding that aldosterone releases sequestered Na are briefly touched upon."}
{"id": "PMID:1080805", "title": "Amino acid movements across the wall of anuran small intestine perfused through the vascular bed.", "content": "1. L-leucine transfer across the wall of the small intestine has been studied in a vascularly perfused preparation from four species of frog. Some properties of the preparation are described. 2. A description is given of the endogenous amino acids appearing in the vascular bed and of the kinetic properties of this washout of endogenous L-leucine. 3. In the steady state of absorption, the transfer function relating the net flux of exogenous L-leucine into the vascular bed to the concentration in the lumen exhibits saturation. Under the conditions of the experiments the apparent concentration in the lumen for half-maximum transfer of L-leucine is found to be 2-1 +/- 0-4 (5) mM. 4. When Na ions are removed from the lumen the transfer of L-leucine into the vascular bed is inhibited. However, the additional removal of Na ions from the fluid in the vascular bed is further inhibitory to the transfer of the amino acid. 5. L-leucine previously absorbed from the lumen appears in the vascular bed in a biphasic fashion. Estimates are deduced of the size of the pool of L-leucine within the tissue which drains into the vascular bed. 6. These results are discussed in relation to previous work on amino acid transport undertaken with various sorts of preparation of small intestine.", "contents": "Amino acid movements across the wall of anuran small intestine perfused through the vascular bed. 1. L-leucine transfer across the wall of the small intestine has been studied in a vascularly perfused preparation from four species of frog. Some properties of the preparation are described. 2. A description is given of the endogenous amino acids appearing in the vascular bed and of the kinetic properties of this washout of endogenous L-leucine. 3. In the steady state of absorption, the transfer function relating the net flux of exogenous L-leucine into the vascular bed to the concentration in the lumen exhibits saturation. Under the conditions of the experiments the apparent concentration in the lumen for half-maximum transfer of L-leucine is found to be 2-1 +/- 0-4 (5) mM. 4. When Na ions are removed from the lumen the transfer of L-leucine into the vascular bed is inhibited. However, the additional removal of Na ions from the fluid in the vascular bed is further inhibitory to the transfer of the amino acid. 5. L-leucine previously absorbed from the lumen appears in the vascular bed in a biphasic fashion. Estimates are deduced of the size of the pool of L-leucine within the tissue which drains into the vascular bed. 6. These results are discussed in relation to previous work on amino acid transport undertaken with various sorts of preparation of small intestine."}
{"id": "PMID:1080806", "title": "The relative contributions of the folds and caveolae to the surface membrane of frog skeletal muscle fibres at different sarcomere lengths.", "content": "The plasmalemmal area of striated muscle fibres is greater than the apparent surface area (A = circumference x length) because of variable folds and the invaginations of the caveolae and T-tubules. Freeze-fracture replicas of the surface membrane of sartorius and semitendinosus muscles from Rana pipiens have been used to determine the numbers and distribution of folds and caveolae at different sarcomere lengths. (1) The plasmalemma folds are variable in size and shape, but are always oriented perpendicular to the long axis of the fibre. The folds vary with stretch, being more prominent at short sarcomere lengths. The caveolae are elliptical invaginations of the plasmalemma which open to the outside by a narrow \"neck\" of approximately 20 nm. The caveolar lumen has an average long dimension of 81.6 +/- 11.7 nm and an average short dimension of 66.9 +/- 7.9 nm. The caveolar \"necks\" only can be seen in freeze-fracture replicas and these are distributed in two circumferential bands on either side of the Z-line, and in longitudinal bands separated by distances of 1-5 mum. In the sartorius muscle, at a sarcomere length of 2.8 mum, there is an average number of thirty-seven caveolae per square micrometer of fibre surface. (2) During passive stretch the opening of folds provides membrane for the necessary increase in surface area up to a sarcomere length of about 3.0 mum. This length is defined as the critical sarcomere length (Sc). The number of caveolae remains constant at all sarcomere lengths less than Sc and thus their \"necks\" have been used as membrane markers to determine the amount of folding at different sarcomere lengths. The membrane area contained in folds and caveolae is expressed as a fraction of the apparent surface area (A). For example, in the sartorius muscle, at a sarcomere length of 2.4 mum, the membrane area, excluding the T-tubules, is: A + 0.1A (folding) + 0.7A (caveolae) = 1.8A. (3) For stretch beyond Sc membrane is provided by the opening of caveolae. At a sarcomere length of about 8 mum all the caveolae are open and the fibres rupture with further stretch. (4) The relative contributions of folds and caveolae vary with sarcomere length in a way that is consistent with assumptions of constant volume and plasmalemma area. The maintenance of constant plasmalemma area, even after excessive stretch, suggests that the plasmalemma is relatively inelastic in this situation.", "contents": "The relative contributions of the folds and caveolae to the surface membrane of frog skeletal muscle fibres at different sarcomere lengths. The plasmalemmal area of striated muscle fibres is greater than the apparent surface area (A = circumference x length) because of variable folds and the invaginations of the caveolae and T-tubules. Freeze-fracture replicas of the surface membrane of sartorius and semitendinosus muscles from Rana pipiens have been used to determine the numbers and distribution of folds and caveolae at different sarcomere lengths. (1) The plasmalemma folds are variable in size and shape, but are always oriented perpendicular to the long axis of the fibre. The folds vary with stretch, being more prominent at short sarcomere lengths. The caveolae are elliptical invaginations of the plasmalemma which open to the outside by a narrow \"neck\" of approximately 20 nm. The caveolar lumen has an average long dimension of 81.6 +/- 11.7 nm and an average short dimension of 66.9 +/- 7.9 nm. The caveolar \"necks\" only can be seen in freeze-fracture replicas and these are distributed in two circumferential bands on either side of the Z-line, and in longitudinal bands separated by distances of 1-5 mum. In the sartorius muscle, at a sarcomere length of 2.8 mum, there is an average number of thirty-seven caveolae per square micrometer of fibre surface. (2) During passive stretch the opening of folds provides membrane for the necessary increase in surface area up to a sarcomere length of about 3.0 mum. This length is defined as the critical sarcomere length (Sc). The number of caveolae remains constant at all sarcomere lengths less than Sc and thus their \"necks\" have been used as membrane markers to determine the amount of folding at different sarcomere lengths. The membrane area contained in folds and caveolae is expressed as a fraction of the apparent surface area (A). For example, in the sartorius muscle, at a sarcomere length of 2.4 mum, the membrane area, excluding the T-tubules, is: A + 0.1A (folding) + 0.7A (caveolae) = 1.8A. (3) For stretch beyond Sc membrane is provided by the opening of caveolae. At a sarcomere length of about 8 mum all the caveolae are open and the fibres rupture with further stretch. (4) The relative contributions of folds and caveolae vary with sarcomere length in a way that is consistent with assumptions of constant volume and plasmalemma area. The maintenance of constant plasmalemma area, even after excessive stretch, suggests that the plasmalemma is relatively inelastic in this situation."}
{"id": "PMID:1080807", "title": "[Action of droperidol on the ionic currents and the contraction of the sino-auricular fiber of frogs. Study of its anti-arrhythmic properties].", "content": "1. The effects of Droperidol on ionic currents were studied in frog atrial fibres by means of double sucrose-gap technique associated with an optical method for recording contraction. 2. Droperidol (5-10(-5) M) caused a significant reduction in the amplitude of the inward currents and slowed their inactivation kinetics. The decrease in the sodium and calcium current intensity may be related to the reduction in the maximum conductance for these ions. 3. A decrease in the amplitude of the first component of contractile response was shown to depend upon a reduction of the intensity of the calcium current. 4. Droperidol affected neither the potassium outward current nor the second component of the contractile response. 5. Repetitive activity induced by long-lasting depolarizing current pulses was suppressed by Droperidol. This suppression may be related to the decrease in the maximum conductance for sodium and calcium ions. 6. Droperidol increased the delay of the reactivation of the inward currents and prolonged the refractory period. 7. These results suggest that the cardiac properties of Droperidol are mainly due to a decreased conductance to Na and Ca ions.", "contents": "[Action of droperidol on the ionic currents and the contraction of the sino-auricular fiber of frogs. Study of its anti-arrhythmic properties]. 1. The effects of Droperidol on ionic currents were studied in frog atrial fibres by means of double sucrose-gap technique associated with an optical method for recording contraction. 2. Droperidol (5-10(-5) M) caused a significant reduction in the amplitude of the inward currents and slowed their inactivation kinetics. The decrease in the sodium and calcium current intensity may be related to the reduction in the maximum conductance for these ions. 3. A decrease in the amplitude of the first component of contractile response was shown to depend upon a reduction of the intensity of the calcium current. 4. Droperidol affected neither the potassium outward current nor the second component of the contractile response. 5. Repetitive activity induced by long-lasting depolarizing current pulses was suppressed by Droperidol. This suppression may be related to the decrease in the maximum conductance for sodium and calcium ions. 6. Droperidol increased the delay of the reactivation of the inward currents and prolonged the refractory period. 7. These results suggest that the cardiac properties of Droperidol are mainly due to a decreased conductance to Na and Ca ions."}
{"id": "PMID:1080808", "title": "[On decrease in spike height of nerve action potential. A comparison between sciatic nerve and ENM (author's transl)].", "content": "Employing ENM and a sciatic nerve removed from bullfrog, configuration of decrease of spike under various experimental conditions was studied. The amplitude of the membrane potential change in excitation was also recorded simultaneously with the decrease of spike in ENM (electric nerve model). Both on the nerve and ENM, with decrease of the resting potential, the amplitude of the spike and the amplitude of the change of the membrane potential were decreased. On the nerve and ENM, the decrease in spike amplitudes was induced by an elevation of the niveau and by a lowering of the top. However, the amplitude of the change of membrane potential of ENM was reduced only from the base of the spike due to an elevation of the niveau, with the top remained at a constant level. These decreases of the spike amplitude and the change of the membrane potential in nerve were consistent with that the electric constitution was the same for nerve and ENM, which is the experimental equivalent circut of the nerve fiber devised by the authors.", "contents": "[On decrease in spike height of nerve action potential. A comparison between sciatic nerve and ENM (author's transl)]. Employing ENM and a sciatic nerve removed from bullfrog, configuration of decrease of spike under various experimental conditions was studied. The amplitude of the membrane potential change in excitation was also recorded simultaneously with the decrease of spike in ENM (electric nerve model). Both on the nerve and ENM, with decrease of the resting potential, the amplitude of the spike and the amplitude of the change of the membrane potential were decreased. On the nerve and ENM, the decrease in spike amplitudes was induced by an elevation of the niveau and by a lowering of the top. However, the amplitude of the change of membrane potential of ENM was reduced only from the base of the spike due to an elevation of the niveau, with the top remained at a constant level. These decreases of the spike amplitude and the change of the membrane potential in nerve were consistent with that the electric constitution was the same for nerve and ENM, which is the experimental equivalent circut of the nerve fiber devised by the authors."}
{"id": "PMID:1080809", "title": "Alpha1-antitrypsin phenotypes in sex chromosome mosaicism.", "content": "We report the alpha1-antitrypsin phenotypes of 21 patients with sex chromosome mosaicism and their parents. The proportion of heterozygotes in the group of parents is significantly greater (p less than 0.01) than that proportion in both control groups. The maternal or paternal contribution to the statistical significance of this observation cannot be distinguished in our small sample of families.", "contents": "Alpha1-antitrypsin phenotypes in sex chromosome mosaicism. We report the alpha1-antitrypsin phenotypes of 21 patients with sex chromosome mosaicism and their parents. The proportion of heterozygotes in the group of parents is significantly greater (p less than 0.01) than that proportion in both control groups. The maternal or paternal contribution to the statistical significance of this observation cannot be distinguished in our small sample of families."}
{"id": "PMID:1080810", "title": "R-factor mediated beta-lactamase production by Haemophilus influenzae.", "content": "Production of beta-lactamase by 15 strains of Haemophilus influenzae has been investigated. All the strains produce a constitutive beta-lactamase, which readily hydrolyses penicillin G, ampicillin, and cephaloridine. The beta-lactamase produced by these strains is indistinguishable from the type-IIIa enzyme commonly found in strains of Escherichia coli. The beta-lactamase gene has been transferred from the enzyme-producing strains of Haemophilus to strains of H. parainfluenzae and a strain of E. coli.", "contents": "R-factor mediated beta-lactamase production by Haemophilus influenzae. Production of beta-lactamase by 15 strains of Haemophilus influenzae has been investigated. All the strains produce a constitutive beta-lactamase, which readily hydrolyses penicillin G, ampicillin, and cephaloridine. The beta-lactamase produced by these strains is indistinguishable from the type-IIIa enzyme commonly found in strains of Escherichia coli. The beta-lactamase gene has been transferred from the enzyme-producing strains of Haemophilus to strains of H. parainfluenzae and a strain of E. coli."}
{"id": "PMID:1080817", "title": "Effects of hypertonic urea solution on the contractility of the bullfrog ventricle.", "content": "The effects of hypertonic urea solution on the excitation-contraction coupling of the bullfrog's heart muscle were investigated. Small strips obtained from the ventricular wall of Rana catesbiana were used. Contractile tension as well as transmembrane action potential were recorded in the single sucrose-gapped arrangement. The muscle weight was measured in order to check the changes in the dimension of muscle fiber both during isotonic and hypertonic perfusions. Light microscopic study was also performed to compare the effect of hyperosmolar urea with that of sucrose. 3.0 T-urea solution exerted a triphasic change in twitch tension during hypertonic perfusion and a biphasic change during the recovery perfusion. An initial rapid decline was restored to the original level within 10 min and after an overshoot a secondary gradual decrease was observed during hypertonic perfusion. In spite of parallel change in the action potential duration with the time to peak tension there was an increase in dp/dt during the restoration phenomenon. The calcium sensitivity was altered in the 3.0 T-urea solution and thus in Ca-free solution the rate constant of the slow component of twitch decline was decreased. The muscle weight remained decreased during the whole period of hypertonic perfusion. Histological study revealed that the fiber bundles soaked in hypertonic urea solution show normal appearance whereas those in sucrose exhibit a marked shrinkage. These results show that urea can penetrate the heart muscle membrane easily and that urea has some augmenting action on the E-C coupling process.", "contents": "Effects of hypertonic urea solution on the contractility of the bullfrog ventricle. The effects of hypertonic urea solution on the excitation-contraction coupling of the bullfrog's heart muscle were investigated. Small strips obtained from the ventricular wall of Rana catesbiana were used. Contractile tension as well as transmembrane action potential were recorded in the single sucrose-gapped arrangement. The muscle weight was measured in order to check the changes in the dimension of muscle fiber both during isotonic and hypertonic perfusions. Light microscopic study was also performed to compare the effect of hyperosmolar urea with that of sucrose. 3.0 T-urea solution exerted a triphasic change in twitch tension during hypertonic perfusion and a biphasic change during the recovery perfusion. An initial rapid decline was restored to the original level within 10 min and after an overshoot a secondary gradual decrease was observed during hypertonic perfusion. In spite of parallel change in the action potential duration with the time to peak tension there was an increase in dp/dt during the restoration phenomenon. The calcium sensitivity was altered in the 3.0 T-urea solution and thus in Ca-free solution the rate constant of the slow component of twitch decline was decreased. The muscle weight remained decreased during the whole period of hypertonic perfusion. Histological study revealed that the fiber bundles soaked in hypertonic urea solution show normal appearance whereas those in sucrose exhibit a marked shrinkage. These results show that urea can penetrate the heart muscle membrane easily and that urea has some augmenting action on the E-C coupling process."}
{"id": "PMID:1080818", "title": "[Clinical aspects of \"Endophthalmitis phacoanaphylactica\" (author's transl)].", "content": "29 cases of Endophthalmitis phacoanaphylactica were diagnosed out of a total of 1300 enucleated eyes examined histologically between 1966 and 1974. Clinically the correct diagnosis was made only once. Most frequent misdiagnoses were: phthisis, recurrent iritis, and panophthalmitis. Important factors for a correct clinical diagnosis are: 1. history of injury at least 2-3 weeks previously, 2. a cataract present at the onset of the disease, 3. an endophthalmitis, often associated with extreme i.o. hypotension. Treatment consists of an immediate and total removal of lens material, regardless of the hypotension or of inflammatory signs. The retinal architecture is usually astonishingly well preserved, even in cases with extreme destruction of the anterior segments.", "contents": "[Clinical aspects of \"Endophthalmitis phacoanaphylactica\" (author's transl)]. 29 cases of Endophthalmitis phacoanaphylactica were diagnosed out of a total of 1300 enucleated eyes examined histologically between 1966 and 1974. Clinically the correct diagnosis was made only once. Most frequent misdiagnoses were: phthisis, recurrent iritis, and panophthalmitis. Important factors for a correct clinical diagnosis are: 1. history of injury at least 2-3 weeks previously, 2. a cataract present at the onset of the disease, 3. an endophthalmitis, often associated with extreme i.o. hypotension. Treatment consists of an immediate and total removal of lens material, regardless of the hypotension or of inflammatory signs. The retinal architecture is usually astonishingly well preserved, even in cases with extreme destruction of the anterior segments."}
{"id": "PMID:1080819", "title": "[Anomaloscopic results in extrem-anomalous trichromats (author's transl)].", "content": "The results in 55 extreme deuteran and 34 extreme protan trichromats examined with the anomaloscope are reported, which make together about 9% of all red-green vision disturbances in a group of Federal Army relatives. Not only the deuteran but also the protan group make fewer mistakes with red colours as with green.", "contents": "[Anomaloscopic results in extrem-anomalous trichromats (author's transl)]. The results in 55 extreme deuteran and 34 extreme protan trichromats examined with the anomaloscope are reported, which make together about 9% of all red-green vision disturbances in a group of Federal Army relatives. Not only the deuteran but also the protan group make fewer mistakes with red colours as with green."}
{"id": "PMID:1080820", "title": "[The action of triglycylvasopressin on control subjects and patients with upper gastrointestinal bleeding (author's transl)].", "content": "Nalpha-glycyl-glycyl-glycyl-(8-lysine)-vasopressin, a hormone analogue with prolonged pharmacological action due to slow release of active nonapeptide by enzyme action in vivo, has been administered to 5 control subjects and to 14 patients actively bleeding from upper gastrointestinal sites. The control subjects showed a prolonged pressor response to 100mug/kg body weight associated with a rise in cardiac output, with no ECG signs of myocardial toxicity. 13 of the 14 bleeding patients showed not only pressor responses and haemodynamic and clinical improvement when administering doses of 20-100 mug/kg, but clear signs of standstill of upper gastrointestinal bleeding.", "contents": "[The action of triglycylvasopressin on control subjects and patients with upper gastrointestinal bleeding (author's transl)]. Nalpha-glycyl-glycyl-glycyl-(8-lysine)-vasopressin, a hormone analogue with prolonged pharmacological action due to slow release of active nonapeptide by enzyme action in vivo, has been administered to 5 control subjects and to 14 patients actively bleeding from upper gastrointestinal sites. The control subjects showed a prolonged pressor response to 100mug/kg body weight associated with a rise in cardiac output, with no ECG signs of myocardial toxicity. 13 of the 14 bleeding patients showed not only pressor responses and haemodynamic and clinical improvement when administering doses of 20-100 mug/kg, but clear signs of standstill of upper gastrointestinal bleeding."}
{"id": "PMID:1080821", "title": "Improved survival after coronary artery surgery in patients with extensive coronary artery disease.", "content": "Survival in patients with ischemic heart disease is closely related to the extent of coronary artery obstruction as determined angiographically. One hundred forty-nine consecutive patients underwent coronary artery bypass surgery from November, 1971, to October, 1974. There were 2 late cardiac deaths, 1 late noncardiac death, and 1 hospital death, an operative mortality rate of 0.7 per cent and a total mortality rate of 2.7 per cent. Coronary angiograms were scored according to the method of Friesinger, Page, and Ross. Fifty-four per cent (80/149) had scores of 10 or greater. Cumulative survival was analyzed according to life-table techniques; in the 80 surgically managed patients with scores of 10 or greater, survival at 3 years was 98 per cent. Friesinger's 47 nonoperated patients with similar angiographic scores had a 3 year cumulative survival of 68 per cent. Although this study compares different groups, the surgical series was composed of older patients (mean age 52 as compared to 44 years), includes 22 patients operated on urgently for preinfarction angina pectoris, and includes 18 patients with abnormal ventricular function. These data suggest that coronary artery bypass surgery can favorably influence prognosis in patients with severe coronary artery disease.", "contents": "Improved survival after coronary artery surgery in patients with extensive coronary artery disease. Survival in patients with ischemic heart disease is closely related to the extent of coronary artery obstruction as determined angiographically. One hundred forty-nine consecutive patients underwent coronary artery bypass surgery from November, 1971, to October, 1974. There were 2 late cardiac deaths, 1 late noncardiac death, and 1 hospital death, an operative mortality rate of 0.7 per cent and a total mortality rate of 2.7 per cent. Coronary angiograms were scored according to the method of Friesinger, Page, and Ross. Fifty-four per cent (80/149) had scores of 10 or greater. Cumulative survival was analyzed according to life-table techniques; in the 80 surgically managed patients with scores of 10 or greater, survival at 3 years was 98 per cent. Friesinger's 47 nonoperated patients with similar angiographic scores had a 3 year cumulative survival of 68 per cent. Although this study compares different groups, the surgical series was composed of older patients (mean age 52 as compared to 44 years), includes 22 patients operated on urgently for preinfarction angina pectoris, and includes 18 patients with abnormal ventricular function. These data suggest that coronary artery bypass surgery can favorably influence prognosis in patients with severe coronary artery disease."}
{"id": "PMID:1080822", "title": "Acute myocardial infarction: a surgical emergency.", "content": "Preservation of viable myocardium is the primary goal of coronary artery surgery. Our total experience with coronary artery bypass grafting is 1,612 patients, operated upon from March 13, 1969, through Jan. 31, 1975 (2.85 per cent over-all mortality rate). Four hundred thirteen patients were operated upon on an emergency basis. Of this group, 96 were having acute myocardial infarctions and 317 were in the preinfarction syndrome. Emergency coronary artery bypass surgery was performed with 5 deaths (5.2 per cent) in the acute myocardial infarction group and 4 deaths in the preinfarction group (1.26 per cent). These patients had a much lower mortality rate than that of medically treated patients in the acute myocardial infarction group. Postoperative catheterization studies on the acute myocardial infarction group showed a 96 per cent rate of primary graft patency. Follow-up studies through 3 years, 10 months show only 1 late death (4 months after the operation). The in-hospital and the first year mortality rates in a medically treated group with acute myocardial infarction were compared with the surgically treated group. The result was a mortality rate of 30 per cent with medical treatment and 6.3 per cent with surgical treatment. Actuarial analysis demonstrated a greater than 20 per cent difference in mortality rate at 1 year, in favor of surgical treatment. The lower surgical mortality coupled with the early and late clinical results prove that emergency coronary bypass is superior therapy in selected patients with acute myocardial infarction.", "contents": "Acute myocardial infarction: a surgical emergency. Preservation of viable myocardium is the primary goal of coronary artery surgery. Our total experience with coronary artery bypass grafting is 1,612 patients, operated upon from March 13, 1969, through Jan. 31, 1975 (2.85 per cent over-all mortality rate). Four hundred thirteen patients were operated upon on an emergency basis. Of this group, 96 were having acute myocardial infarctions and 317 were in the preinfarction syndrome. Emergency coronary artery bypass surgery was performed with 5 deaths (5.2 per cent) in the acute myocardial infarction group and 4 deaths in the preinfarction group (1.26 per cent). These patients had a much lower mortality rate than that of medically treated patients in the acute myocardial infarction group. Postoperative catheterization studies on the acute myocardial infarction group showed a 96 per cent rate of primary graft patency. Follow-up studies through 3 years, 10 months show only 1 late death (4 months after the operation). The in-hospital and the first year mortality rates in a medically treated group with acute myocardial infarction were compared with the surgically treated group. The result was a mortality rate of 30 per cent with medical treatment and 6.3 per cent with surgical treatment. Actuarial analysis demonstrated a greater than 20 per cent difference in mortality rate at 1 year, in favor of surgical treatment. The lower surgical mortality coupled with the early and late clinical results prove that emergency coronary bypass is superior therapy in selected patients with acute myocardial infarction."}
{"id": "PMID:1080823", "title": "Preservation of myocardial ultrastructure.", "content": "This study compares the effects of 2 hour periods of normothermic anoxic arrest, continuous normothermic coronary perfusion, continuous cold coronary perfusion, and topical cooling of the ischemic myocardium on survival and ultramicroscopic structure of the canine myocardium. No animal survived 2 hours of normothermic anoxic arrest. Severe ultrastructural damage was observed in the nuclei, mitochondria, and myofibrils. Topical cooling of the ischemic myocardium to 15 degrees C. markedly improved survival and preservation of the myocardial fine structure. Continuous perfusion of the coronary arteries with cold blood resulted in 100 per cent survival through the experimental period, with preservation of the normal myocardial ultrastructure. The technique of cold coronary perfusion should be particularly useful in cardiac surgical patients in whom maximal preservation of myocardial function is essential for survival.", "contents": "Preservation of myocardial ultrastructure. This study compares the effects of 2 hour periods of normothermic anoxic arrest, continuous normothermic coronary perfusion, continuous cold coronary perfusion, and topical cooling of the ischemic myocardium on survival and ultramicroscopic structure of the canine myocardium. No animal survived 2 hours of normothermic anoxic arrest. Severe ultrastructural damage was observed in the nuclei, mitochondria, and myofibrils. Topical cooling of the ischemic myocardium to 15 degrees C. markedly improved survival and preservation of the myocardial fine structure. Continuous perfusion of the coronary arteries with cold blood resulted in 100 per cent survival through the experimental period, with preservation of the normal myocardial ultrastructure. The technique of cold coronary perfusion should be particularly useful in cardiac surgical patients in whom maximal preservation of myocardial function is essential for survival."}
{"id": "PMID:1080831", "title": "An Haemophilus influenzae mutant which inhibits the growth of HP1c1 phage.", "content": "A strain of Haemophilus influenzae, called hpm- inhibits the growth of phage HP1c1 but not S2. This inhibition is overcome by HP1c1ph mutants. Phage HP1c1 adsorbs normally to hpm- cells but only a small fraction of infected cells produce phage with a normal burst size or become lysogenic. When hpm- strains lysogenic for HP1c1 are induced, 100 percent of the cells yield phage. There is no degradation of phage DNA after infection of hpm- cells and HP1c1 can normally grow when its DNA is introduced into hpm- by transfection. The most probable explanation is that in hpm- cells the penetration of phage DNA is blocked. The hpm- property behaves as as unstable mutation.", "contents": "An Haemophilus influenzae mutant which inhibits the growth of HP1c1 phage. A strain of Haemophilus influenzae, called hpm- inhibits the growth of phage HP1c1 but not S2. This inhibition is overcome by HP1c1ph mutants. Phage HP1c1 adsorbs normally to hpm- cells but only a small fraction of infected cells produce phage with a normal burst size or become lysogenic. When hpm- strains lysogenic for HP1c1 are induced, 100 percent of the cells yield phage. There is no degradation of phage DNA after infection of hpm- cells and HP1c1 can normally grow when its DNA is introduced into hpm- by transfection. The most probable explanation is that in hpm- cells the penetration of phage DNA is blocked. The hpm- property behaves as as unstable mutation."}
{"id": "PMID:1080833", "title": "Tests for the mutagenic actions of a number of chemicals on Haemophilus influenzae with special emphasis on hydrazine.", "content": "A number of chemicals have been tested for their ability to produce novobiocin-resistant mutants in Haemophilus influenzae. Of these, hydrazine (HZ) proved unique because it induced a fairly high incidence of mutation without killing significant numbers of cells at concentrations ranging over nearly four orders of magnitude. Moreover, its dose-effect curve increased very slowly initially and reached a relatively low maximum. It is suggested that HZ may be acting as both a mutagen and an antimutagen in this system. HZ has sometimes been characterized as primarily an inactivating agent rather than as a mutagen. Obviously, quite the opposite is true for H. influenzae. Moreover, a survey of the literature shows that HZ has been used effectively to produce mutations in phage, bacteria, higher plants and Drosophila. There is reason to suspect that it produces mainly single locus mutations rather than chromosomal aberrations, but the amount of information on this point is still very small. The effectiveness of a mutagen can be markedly affected by the criterion of effectiveness used. If a high incidence of mutation with reasonably high cell survival is the criterion, then mutagens that are relatively nontoxic would probably be considered highly effective. In this case, the maximum mutation rate that can be achieved in practice may be controlled either by toxicity or by maxima like the one found for HZ. If, on the other hand, the important criteria are the concentration and exposure time needed to produce a detectable increase in mutation, then some quite toxic mutagens would be considered quite effective even though the maximum mutation that can be produced in practice is low. The first criterion is the one to use in choosing mutagens for detailed analysis of the mutation process, but the second set of criteria may be more suitable for generalizing to other organisms, for example from microorganisms to man, since toxic effects can be quite different in different cell types.", "contents": "Tests for the mutagenic actions of a number of chemicals on Haemophilus influenzae with special emphasis on hydrazine. A number of chemicals have been tested for their ability to produce novobiocin-resistant mutants in Haemophilus influenzae. Of these, hydrazine (HZ) proved unique because it induced a fairly high incidence of mutation without killing significant numbers of cells at concentrations ranging over nearly four orders of magnitude. Moreover, its dose-effect curve increased very slowly initially and reached a relatively low maximum. It is suggested that HZ may be acting as both a mutagen and an antimutagen in this system. HZ has sometimes been characterized as primarily an inactivating agent rather than as a mutagen. Obviously, quite the opposite is true for H. influenzae. Moreover, a survey of the literature shows that HZ has been used effectively to produce mutations in phage, bacteria, higher plants and Drosophila. There is reason to suspect that it produces mainly single locus mutations rather than chromosomal aberrations, but the amount of information on this point is still very small. The effectiveness of a mutagen can be markedly affected by the criterion of effectiveness used. If a high incidence of mutation with reasonably high cell survival is the criterion, then mutagens that are relatively nontoxic would probably be considered highly effective. In this case, the maximum mutation rate that can be achieved in practice may be controlled either by toxicity or by maxima like the one found for HZ. If, on the other hand, the important criteria are the concentration and exposure time needed to produce a detectable increase in mutation, then some quite toxic mutagens would be considered quite effective even though the maximum mutation that can be produced in practice is low. The first criterion is the one to use in choosing mutagens for detailed analysis of the mutation process, but the second set of criteria may be more suitable for generalizing to other organisms, for example from microorganisms to man, since toxic effects can be quite different in different cell types."}
{"id": "PMID:1080834", "title": "Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells.", "content": "Since patients with myeloma have serious abnormalities of humoral immunity, we applied an in vitro assay to determine the capacity of B lymphocytes to mature into immunoglobulin-secreting cells. In peripheral blood lymphocytes from 22 normal persons, geometric mean immunoglobulin synthesis was 4910 ng for IgM, 1270 ng for IgA and 1625 ng for IgG. The synthesis rates of peripheral blood lymphocytes of 22 patients with myeloma were 458 ng for IgM, 321 ng for IgA and 218 ng for IgG. Circulating mononuclear cells from three of six patients tested suppressed polyclonal immunoglobulin synthesis by cocultured normal lymphocytes. Suppressive activity was not mediated by purified T cells alone. Removal of phagocytic mononuclear cells from lymphocyte populations of one patient nullified suppressive activity. Removal of phagocytic mononuclear cells from lymphocyte populations of a second patient led to a nearly 10-fold increase in polyclonal immunoglobulin synthesis. Therefore, host suppressor cells may play a part in the decreased capacity of B lymphocytes to secret immunoglobulin in certain patients with myeloma.", "contents": "Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. Since patients with myeloma have serious abnormalities of humoral immunity, we applied an in vitro assay to determine the capacity of B lymphocytes to mature into immunoglobulin-secreting cells. In peripheral blood lymphocytes from 22 normal persons, geometric mean immunoglobulin synthesis was 4910 ng for IgM, 1270 ng for IgA and 1625 ng for IgG. The synthesis rates of peripheral blood lymphocytes of 22 patients with myeloma were 458 ng for IgM, 321 ng for IgA and 218 ng for IgG. Circulating mononuclear cells from three of six patients tested suppressed polyclonal immunoglobulin synthesis by cocultured normal lymphocytes. Suppressive activity was not mediated by purified T cells alone. Removal of phagocytic mononuclear cells from lymphocyte populations of one patient nullified suppressive activity. Removal of phagocytic mononuclear cells from lymphocyte populations of a second patient led to a nearly 10-fold increase in polyclonal immunoglobulin synthesis. Therefore, host suppressor cells may play a part in the decreased capacity of B lymphocytes to secret immunoglobulin in certain patients with myeloma."}
{"id": "PMID:1080840", "title": "Aneurysm of the internal auditory artery revealed by a partial cerebellopontine angle syndrome.", "content": "A case of aneurysm of the distal portion of the middle cerebellar artery is described. The aneurysm was clipped and, as the neck was long, removed. The case is of interest not only because of its rarity but also because of the unusual course of the symptoms, the difficulty of identifying and locating the malformation, overcome with the aid of modern radiological techniques, and because of the success of surgical treatment.", "contents": "Aneurysm of the internal auditory artery revealed by a partial cerebellopontine angle syndrome. A case of aneurysm of the distal portion of the middle cerebellar artery is described. The aneurysm was clipped and, as the neck was long, removed. The case is of interest not only because of its rarity but also because of the unusual course of the symptoms, the difficulty of identifying and locating the malformation, overcome with the aid of modern radiological techniques, and because of the success of surgical treatment."}
{"id": "PMID:1080842", "title": "The chain guide technique for selective ventriculography.", "content": "A technique for selective ventriculography using a chain as a guide, utilizing gravity has been previously described by the author. Additional details of manipulation and injections are now reported. The cisterns have also been reached with the technique and narrow spaces as aqueductal stenosis has been passed through with the chain.", "contents": "The chain guide technique for selective ventriculography. A technique for selective ventriculography using a chain as a guide, utilizing gravity has been previously described by the author. Additional details of manipulation and injections are now reported. The cisterns have also been reached with the technique and narrow spaces as aqueductal stenosis has been passed through with the chain."}
{"id": "PMID:1080844", "title": "Maternal mortality in a teaching hospital in southern India. A 13-year study.", "content": "During the 13 years 1960-1972, in a teaching hospital that serves a predominantly rural and semiurban population in southern India, there were 74,384 deliveries and 1245 maternal deaths, a maternal mortality rate of 16.7 per 1000 births. Direct obstetric factors caused 854 (65.5%) of these deaths. The leading indirect or associated causes of maternal deaths were anemia, cerebrovascular accidents, and infectious hepatitis. During the past 13 years, monthly maternal mortality meetings have helped to reduce the incidence of avoidable factors in maternal deaths among patients from the city but not among those brought from the surrounding countryside. The important causes of maternal deaths in this developing country, and their prevention, are individually discussed.", "contents": "Maternal mortality in a teaching hospital in southern India. A 13-year study. During the 13 years 1960-1972, in a teaching hospital that serves a predominantly rural and semiurban population in southern India, there were 74,384 deliveries and 1245 maternal deaths, a maternal mortality rate of 16.7 per 1000 births. Direct obstetric factors caused 854 (65.5%) of these deaths. The leading indirect or associated causes of maternal deaths were anemia, cerebrovascular accidents, and infectious hepatitis. During the past 13 years, monthly maternal mortality meetings have helped to reduce the incidence of avoidable factors in maternal deaths among patients from the city but not among those brought from the surrounding countryside. The important causes of maternal deaths in this developing country, and their prevention, are individually discussed."}
{"id": "PMID:1080846", "title": "[Congenital hereditary corneal dystrophy associated with various extraoculary anomalies].", "content": "Two subjects (brother and sister), children of consanguineous parents, showed a typical congenital corneal dystrophy associated with mental retardation and a bilateral malformation of the little finger. One of them, a boy of 10, was fat and showed a hearing loss for high tones. His corneal opacity diminished during 7 years observation. Seeing that the affected tissues were of mesenchymal origin, the authors conclude that the syndrome was mesodermal.", "contents": "[Congenital hereditary corneal dystrophy associated with various extraoculary anomalies]. Two subjects (brother and sister), children of consanguineous parents, showed a typical congenital corneal dystrophy associated with mental retardation and a bilateral malformation of the little finger. One of them, a boy of 10, was fat and showed a hearing loss for high tones. His corneal opacity diminished during 7 years observation. Seeing that the affected tissues were of mesenchymal origin, the authors conclude that the syndrome was mesodermal."}
{"id": "PMID:1080847", "title": "Petechial hemorrhages of the soft palate secondary to fellatio.", "content": "A 25-year-old white woman was evaluated for \"spots\" on the roof of her mouth of 2 days' duration. The initial history, physical examination, and laboratory data all failed to indicate any cause for the bleeding diathesis. Therefore, a more intensive history was initiated which disclosed a probable case for fellatio as the cause of the spots. The intraoral examination showed the spots to be petechial hemorrhages located on the soft palate in the area of the insertion of the tendons of the tensor veli palatini and levator veli palatini muscles. The mechanism of injury producing these petechiae was postulated to be the combined muscular actions initiated reflexively through tactile stimulation and the negative pressure created through irrumation, both acting simultaneously on the mucosa of the soft palate. This case is presented to illustrate an addition that should be made to the differential etiologic factors of intraoral petechiae.", "contents": "Petechial hemorrhages of the soft palate secondary to fellatio. A 25-year-old white woman was evaluated for \"spots\" on the roof of her mouth of 2 days' duration. The initial history, physical examination, and laboratory data all failed to indicate any cause for the bleeding diathesis. Therefore, a more intensive history was initiated which disclosed a probable case for fellatio as the cause of the spots. The intraoral examination showed the spots to be petechial hemorrhages located on the soft palate in the area of the insertion of the tendons of the tensor veli palatini and levator veli palatini muscles. The mechanism of injury producing these petechiae was postulated to be the combined muscular actions initiated reflexively through tactile stimulation and the negative pressure created through irrumation, both acting simultaneously on the mucosa of the soft palate. This case is presented to illustrate an addition that should be made to the differential etiologic factors of intraoral petechiae."}
{"id": "PMID:1080848", "title": "Palatal erythema: another etiologic factor.", "content": "The authors present three cases which created diagnostic problems. The etiology of these is, in the authors' experience, unique and not generally known. It is suggested that this etiology be considered whenever palatal erythema, ecchymoses, or petechiae are seen.", "contents": "Palatal erythema: another etiologic factor. The authors present three cases which created diagnostic problems. The etiology of these is, in the authors' experience, unique and not generally known. It is suggested that this etiology be considered whenever palatal erythema, ecchymoses, or petechiae are seen."}
{"id": "PMID:1080857", "title": "Endotoxin-induced release of colony-stimulating activity in man.", "content": "Six healthy adult volunteers were injected intravenously with Pseudomonas endotoxin (Piromen) to determine effects on serum colony-stimulating activity (CSA). Serum was assayed with a double-layer agar cloning technique using normal human bone marrow as the target cell preparation. A marked increase in serum CSA was noted after endotoxin administration lasting up to 180 min. The colonies formed in response to postendotoxin serum were smaller than those seen with leukocyte feeder layers but were otherwise morphologically normal. These studies suggest that granulopoietic humoral factors may be modulated in man by endotoxin administration.", "contents": "Endotoxin-induced release of colony-stimulating activity in man. Six healthy adult volunteers were injected intravenously with Pseudomonas endotoxin (Piromen) to determine effects on serum colony-stimulating activity (CSA). Serum was assayed with a double-layer agar cloning technique using normal human bone marrow as the target cell preparation. A marked increase in serum CSA was noted after endotoxin administration lasting up to 180 min. The colonies formed in response to postendotoxin serum were smaller than those seen with leukocyte feeder layers but were otherwise morphologically normal. These studies suggest that granulopoietic humoral factors may be modulated in man by endotoxin administration."}
{"id": "PMID:1080858", "title": "In utero sensitization with influenza virus in man.", "content": "Following a community-wide epidemic of influenza A/England virus infection, 64 cord blood samples were evaluated for their fetal serum and lymphocyte responses to this virus. Cord sera were tested for HI antibody vs. A/England with and without 2-ME. A fourfold or greater reduction in titer was observed in four, indicating fetal IgM antibody responses. In addition, lymphocyte samples from three of 16 tested showed proliferative responses to influenza A suggesting fetal lymphocyte sensitization with influenza virus. These data suggest that, in some pregnancies, influenza virus is capable of transplacental sensitization of the fetus.", "contents": "In utero sensitization with influenza virus in man. Following a community-wide epidemic of influenza A/England virus infection, 64 cord blood samples were evaluated for their fetal serum and lymphocyte responses to this virus. Cord sera were tested for HI antibody vs. A/England with and without 2-ME. A fourfold or greater reduction in titer was observed in four, indicating fetal IgM antibody responses. In addition, lymphocyte samples from three of 16 tested showed proliferative responses to influenza A suggesting fetal lymphocyte sensitization with influenza virus. These data suggest that, in some pregnancies, influenza virus is capable of transplacental sensitization of the fetus."}
{"id": "PMID:1080868", "title": "Influence of extracellular calcium and a water-soluble carboxyl group reagent on cholinergic drug-receptor interactions in skeletal muscle.", "content": "Contractions of frog rectus abdominus muscles induced by suxamethonium or decamethonium, and to a lesser extent by carbamylcholine, were decreased as the extracellular calcium concentration was increased. The inhibition of all three agonist responses by 1.1 mM calcium was competitive. The contractions induced by bis-onium compounds, but not carbamylcholine, were further antagonized at higher calcium concentrations (4.4 mM) in a noncompetitive manner. This latter effect of calcium may be due to antagonism of bis-onium compounds at a peripheral anionic site. Carboxyl group carbodiimide reagents inhibited muscle contractions, and this inhibition was slowly reversible. The extent of the inhibition was increased, and its recovery delayed, by prior exposure of the muscle to an agonist. The results support suggestions that receptor activation initially involves displacement of membrane calcium. The study provides further evidence that interaction of agonists iwth nicotinic receptors results in structural changes, possibly related to increased ion flow.", "contents": "Influence of extracellular calcium and a water-soluble carboxyl group reagent on cholinergic drug-receptor interactions in skeletal muscle. Contractions of frog rectus abdominus muscles induced by suxamethonium or decamethonium, and to a lesser extent by carbamylcholine, were decreased as the extracellular calcium concentration was increased. The inhibition of all three agonist responses by 1.1 mM calcium was competitive. The contractions induced by bis-onium compounds, but not carbamylcholine, were further antagonized at higher calcium concentrations (4.4 mM) in a noncompetitive manner. This latter effect of calcium may be due to antagonism of bis-onium compounds at a peripheral anionic site. Carboxyl group carbodiimide reagents inhibited muscle contractions, and this inhibition was slowly reversible. The extent of the inhibition was increased, and its recovery delayed, by prior exposure of the muscle to an agonist. The results support suggestions that receptor activation initially involves displacement of membrane calcium. The study provides further evidence that interaction of agonists iwth nicotinic receptors results in structural changes, possibly related to increased ion flow."}
{"id": "PMID:1080869", "title": "[Mucous membrane specific protease inhibitors in bronchial mucus in severe chronic obstructive bronchitis and in alpha 1-antitrypsin deficiency syndrome].", "content": "In the bronchial mucus of 40 patients with chronic obstructive airway diseases we measured proteolytic activities, the total protein concentrations, alpha1-antitrypsin, alpha1-antichymotrypsin, and the free and bound proteinase inhibitors together with the total proteinase inhibition against trypsin and chymotrypsin. Without exception we always found free proteinase inhibitors together with proteolytic activities. The free-to-bound inhibitor rate was approximately 1:1 alpha1-Antitrypsin and alpha1-antichymotrypsin was measured in sputum only in very low concentrations. One patient with alpha1-anti-trypsin deficiency had no alpha1-antitrypsin, but high concentrations of total proteinase inhibitor-free and bound being in the same relation - in his bronchial mucus. In the alveolar part of the lung, the humoral proteinase inhibitors were effective. In the bronchial part of the lung the specific mucosal inhibitors had the decided importance. The proteinase inhibition of the mucosa-specific inhibitors is probably of great importance for the pathogenesis of airway obstruction, while the humoral proteinase inhibitors are responsible for the pathogenesis of emphysema.", "contents": "[Mucous membrane specific protease inhibitors in bronchial mucus in severe chronic obstructive bronchitis and in alpha 1-antitrypsin deficiency syndrome]. In the bronchial mucus of 40 patients with chronic obstructive airway diseases we measured proteolytic activities, the total protein concentrations, alpha1-antitrypsin, alpha1-antichymotrypsin, and the free and bound proteinase inhibitors together with the total proteinase inhibition against trypsin and chymotrypsin. Without exception we always found free proteinase inhibitors together with proteolytic activities. The free-to-bound inhibitor rate was approximately 1:1 alpha1-Antitrypsin and alpha1-antichymotrypsin was measured in sputum only in very low concentrations. One patient with alpha1-anti-trypsin deficiency had no alpha1-antitrypsin, but high concentrations of total proteinase inhibitor-free and bound being in the same relation - in his bronchial mucus. In the alveolar part of the lung, the humoral proteinase inhibitors were effective. In the bronchial part of the lung the specific mucosal inhibitors had the decided importance. The proteinase inhibition of the mucosa-specific inhibitors is probably of great importance for the pathogenesis of airway obstruction, while the humoral proteinase inhibitors are responsible for the pathogenesis of emphysema."}
{"id": "PMID:1080878", "title": "Free rheumatoid factor in dental periapical lesions and gingivae of patients with rheumatoid disease.", "content": "To determine whether evidence of rheumatoid inflammation, in the form of free rheumatoid factor, might be found in the teeth-supporting tissues of patients with known rheumatoid disease, tissues from the dental periapical lesions of one group of 50 rheumatoid and 23 control patients, and from the marginal gingivae of a second group of 58 rheumatoid patients were examined by the direct immunofluorescence technique that employed fluroesceinisothiocyanate (FITC)-labelled aggregated human IgG. The gingival tissues contained no free rheumatoid factor. Free rheumatoid factor-producing plasma cells were, however, detected in the  dental periapical lesions of 3 of the 50 rheumatoid patients, i.e. in 6%, and in 1 of the control patients i.e., in 4%. This control patient had suffered from nephritis 10 months prior to the investigation. Because free rheumatoid factor did occur, albeit infrequently, in the dental periapical lesions of rheumatoid patients, a search for IgG rheumatoid factor, known to occur in greater abundance than the IgM type although \"hidden\", was indicated.", "contents": "Free rheumatoid factor in dental periapical lesions and gingivae of patients with rheumatoid disease. To determine whether evidence of rheumatoid inflammation, in the form of free rheumatoid factor, might be found in the teeth-supporting tissues of patients with known rheumatoid disease, tissues from the dental periapical lesions of one group of 50 rheumatoid and 23 control patients, and from the marginal gingivae of a second group of 58 rheumatoid patients were examined by the direct immunofluorescence technique that employed fluroesceinisothiocyanate (FITC)-labelled aggregated human IgG. The gingival tissues contained no free rheumatoid factor. Free rheumatoid factor-producing plasma cells were, however, detected in the  dental periapical lesions of 3 of the 50 rheumatoid patients, i.e. in 6%, and in 1 of the control patients i.e., in 4%. This control patient had suffered from nephritis 10 months prior to the investigation. Because free rheumatoid factor did occur, albeit infrequently, in the dental periapical lesions of rheumatoid patients, a search for IgG rheumatoid factor, known to occur in greater abundance than the IgM type although \"hidden\", was indicated."}
{"id": "PMID:1080879", "title": "An evaluation of two new screening tests for rheumatoid factors.", "content": "Two further commercial screening tests for rheumatoid factors have been evaluated using both the sheep cell agglutination (SCAT) test and the Rheumaton slide test used routinely in this laboratory. In the group of sera tested, the Rheumaton test detected all sera with elevated SCAT titres; the Searle RA Latex test failed to detect a few of these sera. The Rheumaton test gave many misleading positive reactions with sera having insignificant SCAT titres. Far fewer misleading positive results were obtained with the Searle test. The Rythrotex test gave results similar to Rheumaton but was very difficult to use.", "contents": "An evaluation of two new screening tests for rheumatoid factors. Two further commercial screening tests for rheumatoid factors have been evaluated using both the sheep cell agglutination (SCAT) test and the Rheumaton slide test used routinely in this laboratory. In the group of sera tested, the Rheumaton test detected all sera with elevated SCAT titres; the Searle RA Latex test failed to detect a few of these sera. The Rheumaton test gave many misleading positive reactions with sera having insignificant SCAT titres. Far fewer misleading positive results were obtained with the Searle test. The Rythrotex test gave results similar to Rheumaton but was very difficult to use."}
{"id": "PMID:1080876", "title": "[HL-A and psoriatic rheumatism].", "content": "The HL-A groups, including the characteristics of 25 antigens, were measured in 61 patients suffering from psoriatic rheumatism, among them 9 with central forms (pelvispondylitis) and 52 with peripheral forms (polyarthritis with or without sacroileitis). Analysis of the results and comparison of them with those of other workers led to the following conclusions: 1) Central psoriatic rheumatism is associated mainly with W27 and Da31, less closely with HL-A13, and slightly or not at all with W17. 2) Peripheral psoriatic rheumatism has the same relationship with the HL-A system as has psoriasis without anthropathy. It is associated mainly with W17, less closely with Da31 and HL-A13, and slightly or not at all with W27. 3) By their relationship to the HL-A system, the two varieties of psoriatic rheumatism may be clearly distinguished from one another and from other inflammatory rheumatisms. The HL-A groupings are of considerable value in the differential diagnosis between central psoriatic rheumatism, peripheral psoriatic rheumatism, ankylosing spondylarthritis, the Fiessinger-Leroy-Reiter syndrome and rheumatoid arthritis.", "contents": "[HL-A and psoriatic rheumatism]. The HL-A groups, including the characteristics of 25 antigens, were measured in 61 patients suffering from psoriatic rheumatism, among them 9 with central forms (pelvispondylitis) and 52 with peripheral forms (polyarthritis with or without sacroileitis). Analysis of the results and comparison of them with those of other workers led to the following conclusions: 1) Central psoriatic rheumatism is associated mainly with W27 and Da31, less closely with HL-A13, and slightly or not at all with W17. 2) Peripheral psoriatic rheumatism has the same relationship with the HL-A system as has psoriasis without anthropathy. It is associated mainly with W17, less closely with Da31 and HL-A13, and slightly or not at all with W27. 3) By their relationship to the HL-A system, the two varieties of psoriatic rheumatism may be clearly distinguished from one another and from other inflammatory rheumatisms. The HL-A groupings are of considerable value in the differential diagnosis between central psoriatic rheumatism, peripheral psoriatic rheumatism, ankylosing spondylarthritis, the Fiessinger-Leroy-Reiter syndrome and rheumatoid arthritis."}
{"id": "PMID:1080877", "title": "[Presence of cryoprecipitates in the serum of patients with rheumatoid arthritis].", "content": "Serum samples from 140 controls (C) and from 72 patients suffering from defined rheumatoid polyarthritis (RP) were tested for cryoglobulins (CG). CG were found in 19.7 percent of C and 55 percent of RP : a highly significant difference (p less than or equal to 0.001). In the controls there was no correlation between the CG and age or sex. The RP with cryoglobulins seemed to have more inflammation, but systemic manifestations were not any more frequent than in the cryonegative RP. In the RP a correlation was noted between the presence of CG and the following parameters : increase in the sedimentation rate, rise in serum immunoglobulins, IgA and IgC, presence of antinuclear factors, and drop in the C4 fraction of complement. There was no correlation with the presence of the rheumatoid factor in the serum. In 33 cryoprecipitates in the RP, 7 contained IgG, 1 IgM, 22 IgM-IgG -- of which 11 contained C1q and 16 rheumatoid factor -- and 3 contained IgA-IgM-IgG -- of which 1 contained C1q and 2 rheumatoid factor. Some of the biochemical data support the hypothesis that likens these cryoglobulins to immune complexes.", "contents": "[Presence of cryoprecipitates in the serum of patients with rheumatoid arthritis]. Serum samples from 140 controls (C) and from 72 patients suffering from defined rheumatoid polyarthritis (RP) were tested for cryoglobulins (CG). CG were found in 19.7 percent of C and 55 percent of RP : a highly significant difference (p less than or equal to 0.001). In the controls there was no correlation between the CG and age or sex. The RP with cryoglobulins seemed to have more inflammation, but systemic manifestations were not any more frequent than in the cryonegative RP. In the RP a correlation was noted between the presence of CG and the following parameters : increase in the sedimentation rate, rise in serum immunoglobulins, IgA and IgC, presence of antinuclear factors, and drop in the C4 fraction of complement. There was no correlation with the presence of the rheumatoid factor in the serum. In 33 cryoprecipitates in the RP, 7 contained IgG, 1 IgM, 22 IgM-IgG -- of which 11 contained C1q and 16 rheumatoid factor -- and 3 contained IgA-IgM-IgG -- of which 1 contained C1q and 2 rheumatoid factor. Some of the biochemical data support the hypothesis that likens these cryoglobulins to immune complexes."}
{"id": "PMID:1080880", "title": "[Prinzmetal's angor. Apropos of 4 cases. Review of the literature].", "content": "In the light of 4 personal observations of PPPRINZMETAL's angina, a review has been conducted of the literature in the 15 years since the condition was first described. Although the formal diagnostic criteria for this form of angina simultaneously clinical, biological and electrical - anginal attacks occurring at rest, often at night, during which elevation of the ST segment is recorded which disappears at the end of the attack without any significant rise in enzyme levels (SGOT and CPK) - the frontiers of the syndrome appear to have widened since PRINZMETAL's description: - Severe proximal stenosis of the coronary arteries is not obligatory; they may be only slightly damaged or even healthy. - Prinzmetal's angina is by no means always \"spontaneous\" but is often induced, either by psychic factors, which explain the fixed time of the attacks, or by organic factors, e.g. cold drinks (Observation No.2). In this event it would appear safer to speak of angina or rest as opposed to angina of effort. - In contrast to what PRINZMETAL thought, effort tests may sometimes induce angina-type pain with elevation of the ST segment, and here the borderline between this syndrome and conventional angina with ST segment elevation after effort test (5% of cases) is less clear-cut. The two nosologic entities probably reflect the same physiopathological situation, i.e. acute myocardial ischemia, and may represent the same affection in different phases of development. The prognosis is equally bad. - Attacks of rinzmetal's angina are often accompanied by severe and sometimes fatal disorders of rhythm, and this influences the therapeutic approach. - The coronary spasm posited by PRINZMETAL and others before the advent of coronarography is indeed, in the majority of cases, the immediate cause of myocardial ischemia and anginal pain, without any preliminary increase in the energy requirements of the heart as in the conventional anginal attack. - A vasoactive substance present in the circulating blood at the beginning of the affection, which may be degraded and subsequently disappear and may be secreted by the pathologic coronary artery, was demonstrated in observation No. 4: this may, in conjunction with vagal hypertonia, be the causative factor in coronary spasm. Study of its pharmacodynamic properties is now in progress.", "contents": "[Prinzmetal's angor. Apropos of 4 cases. Review of the literature]. In the light of 4 personal observations of PPPRINZMETAL's angina, a review has been conducted of the literature in the 15 years since the condition was first described. Although the formal diagnostic criteria for this form of angina simultaneously clinical, biological and electrical - anginal attacks occurring at rest, often at night, during which elevation of the ST segment is recorded which disappears at the end of the attack without any significant rise in enzyme levels (SGOT and CPK) - the frontiers of the syndrome appear to have widened since PRINZMETAL's description: - Severe proximal stenosis of the coronary arteries is not obligatory; they may be only slightly damaged or even healthy. - Prinzmetal's angina is by no means always \"spontaneous\" but is often induced, either by psychic factors, which explain the fixed time of the attacks, or by organic factors, e.g. cold drinks (Observation No.2). In this event it would appear safer to speak of angina or rest as opposed to angina of effort. - In contrast to what PRINZMETAL thought, effort tests may sometimes induce angina-type pain with elevation of the ST segment, and here the borderline between this syndrome and conventional angina with ST segment elevation after effort test (5% of cases) is less clear-cut. The two nosologic entities probably reflect the same physiopathological situation, i.e. acute myocardial ischemia, and may represent the same affection in different phases of development. The prognosis is equally bad. - Attacks of rinzmetal's angina are often accompanied by severe and sometimes fatal disorders of rhythm, and this influences the therapeutic approach. - The coronary spasm posited by PRINZMETAL and others before the advent of coronarography is indeed, in the majority of cases, the immediate cause of myocardial ischemia and anginal pain, without any preliminary increase in the energy requirements of the heart as in the conventional anginal attack. - A vasoactive substance present in the circulating blood at the beginning of the affection, which may be degraded and subsequently disappear and may be secreted by the pathologic coronary artery, was demonstrated in observation No. 4: this may, in conjunction with vagal hypertonia, be the causative factor in coronary spasm. Study of its pharmacodynamic properties is now in progress."}
{"id": "PMID:1080881", "title": "Ultrasensitive chemosensory responses by a protozoan to epinephrine and other neurochemicals.", "content": "A behavioral assay was developed based on differential tendency of a protozoan to attach to an agar gel containing the test substance. The heterotrophic marine dinoflagellate Crypthecodinium (Gyrodinium) cohnii responded negatively (less tendency to attach) to epinephrine at concentrations above 5 X 10(-15)M and to norepinephrine at concentrations above 5 X 10(-9)M. Response to choline as choline H2 citrate, choline bitartrate, and choline chloride was negative above 10(-7)M, but response to the choline analog carbachol was positve (greater tendency to attach) in the range 5 X 10(-6) to 5 X 10(-4)M. Other responses to neurochemicals at comparable concentrations were: dopa, betaine, and glycine--positive; L-glutamic acid, tryptophan, putrescine, and taurine--negative. Serotonin was inert, responses to tyrosine and gamma-aminobutyric acid were variable, and phenylalanine (6 X 10(-3)M) and 5-hydroxytryptophan (5 X 10(-4)M) were negative only at the highest concentrations tested.", "contents": "Ultrasensitive chemosensory responses by a protozoan to epinephrine and other neurochemicals. A behavioral assay was developed based on differential tendency of a protozoan to attach to an agar gel containing the test substance. The heterotrophic marine dinoflagellate Crypthecodinium (Gyrodinium) cohnii responded negatively (less tendency to attach) to epinephrine at concentrations above 5 X 10(-15)M and to norepinephrine at concentrations above 5 X 10(-9)M. Response to choline as choline H2 citrate, choline bitartrate, and choline chloride was negative above 10(-7)M, but response to the choline analog carbachol was positve (greater tendency to attach) in the range 5 X 10(-6) to 5 X 10(-4)M. Other responses to neurochemicals at comparable concentrations were: dopa, betaine, and glycine--positive; L-glutamic acid, tryptophan, putrescine, and taurine--negative. Serotonin was inert, responses to tyrosine and gamma-aminobutyric acid were variable, and phenylalanine (6 X 10(-3)M) and 5-hydroxytryptophan (5 X 10(-4)M) were negative only at the highest concentrations tested."}
{"id": "PMID:1080882", "title": "Differentiation of red blood cells in vitro.", "content": "Differentiation of red blood cells occurs in organ cultures of both livers and kidney tissue from tadpoles of the bullfrog Rana catesbeiana. Our evidence indicates that different red blood cell lines are produced by the two tissues and that these different cell lines contain different tadpole hemoglobins.", "contents": "Differentiation of red blood cells in vitro. Differentiation of red blood cells occurs in organ cultures of both livers and kidney tissue from tadpoles of the bullfrog Rana catesbeiana. Our evidence indicates that different red blood cell lines are produced by the two tissues and that these different cell lines contain different tadpole hemoglobins."}
{"id": "PMID:1080883", "title": "Studies on the pathogenicity of Ancylostoma ceylanicum. I. Blood loss in experimental dogs.", "content": "Blood loss caused by A. ceylancium was determined in 13 dogs using 51Cr-labelled red cells. Blood loss was first detected in the faeces on the 10th to 13th day after cutaneous and 8th to 16th day after oral infection respectively. The mean blood loss was found to be 0.033 ml/worm/day and 0.038 ml/worm/day in these 2 groups of dogs respectively. There was a direct relationship between the number of worm and the amount of blood loss. The blood loss expressed as ml per worm per day showed a reverse relationship with the number of worms recovered. Considering a large amount of blood loss and the decrease in haematological values in these dogs, A. ceylanicum should be considered as one of the hookworm species of medical importance.", "contents": "Studies on the pathogenicity of Ancylostoma ceylanicum. I. Blood loss in experimental dogs. Blood loss caused by A. ceylancium was determined in 13 dogs using 51Cr-labelled red cells. Blood loss was first detected in the faeces on the 10th to 13th day after cutaneous and 8th to 16th day after oral infection respectively. The mean blood loss was found to be 0.033 ml/worm/day and 0.038 ml/worm/day in these 2 groups of dogs respectively. There was a direct relationship between the number of worm and the amount of blood loss. The blood loss expressed as ml per worm per day showed a reverse relationship with the number of worms recovered. Considering a large amount of blood loss and the decrease in haematological values in these dogs, A. ceylanicum should be considered as one of the hookworm species of medical importance."}
{"id": "PMID:1080884", "title": "Tuberculosis in Black children. A retrospective analysis of severe cases.", "content": "A retrospective study of 176 Black children requiring hospital treatment for tuberculosis showed that infants and children under the age of 3 years are especially susceptible. Extensive and disseminated disease is usually present at the time of diagnosis, which was sometimes made difficult by a low tuberculin sensitivity caused by malnutrition, measles, or overwhelming infection. Almost a quarter of the patients had received BCG. Approximately half the patients had disseminated disease; of these, 40% had tuberculous meningitis and 12% died, whereas the mortality of those with localised disease was 3%.", "contents": "Tuberculosis in Black children. A retrospective analysis of severe cases. A retrospective study of 176 Black children requiring hospital treatment for tuberculosis showed that infants and children under the age of 3 years are especially susceptible. Extensive and disseminated disease is usually present at the time of diagnosis, which was sometimes made difficult by a low tuberculin sensitivity caused by malnutrition, measles, or overwhelming infection. Almost a quarter of the patients had received BCG. Approximately half the patients had disseminated disease; of these, 40% had tuberculous meningitis and 12% died, whereas the mortality of those with localised disease was 3%."}
{"id": "PMID:1080885", "title": "[Myelopoiesis--a kinetic approach].", "content": "The mechanisms of haemopoietic cellular proliferation are more clearly understood when the granylocytic, monocytic and macrophagic elements of the bone marrow are studied by means of in vitro cultures. Better physiological insight into stimulating and inhibitory factors is obtained in this way. These studies are of diagnostic, therapeutic and prognostic importance in the clinical handling of myeloid leukaemia dn neutropenia. It can be accepted today that the concept of myeloid leukaemia as a neoplastic process with an increased production of autonomous cell populations is to a large extent outdated, and these cells can be induced in vitro to differentiate into mature polymorphs. In the past it has been demonstrated that in vitro successes are followed by in vivo results, and in particular it is hoped that with the development of techniques for concentration of colony-stimulating factor, that this might be of therapeutic advantage in selected leukaemia patients in the future.", "contents": "[Myelopoiesis--a kinetic approach]. The mechanisms of haemopoietic cellular proliferation are more clearly understood when the granylocytic, monocytic and macrophagic elements of the bone marrow are studied by means of in vitro cultures. Better physiological insight into stimulating and inhibitory factors is obtained in this way. These studies are of diagnostic, therapeutic and prognostic importance in the clinical handling of myeloid leukaemia dn neutropenia. It can be accepted today that the concept of myeloid leukaemia as a neoplastic process with an increased production of autonomous cell populations is to a large extent outdated, and these cells can be induced in vitro to differentiate into mature polymorphs. In the past it has been demonstrated that in vitro successes are followed by in vivo results, and in particular it is hoped that with the development of techniques for concentration of colony-stimulating factor, that this might be of therapeutic advantage in selected leukaemia patients in the future."}
{"id": "PMID:1080886", "title": "Onyalai--the possible involvement of a mycotoxin produced by Phoma sorghina in the aetiology.", "content": "A number of species of fungi were isolated from millet (Pennisetum typhoides Staph and Hubb) and grain sorghum (Sorghum vulgare Pers) obtained from the households of patients suffering from onyalai. Several proved to be toxic to rats and chickens, including Phoma sorghina (Sacc.) which was the predominating fungus. Cultures of P. sorghina grown on maize and wheat were added to standard rations at concentrations of 5-30%. When fed to day-old New Hampshire chickens, the majority died within 4 days, exhibiting traces of blood on the beak and the cloaca. Rats given 10% or more of the mouldered material in the diet developed thrombocytopenia after 14 days which was followed by haematuria, epistaxis, melaena, and death. Pathological, including histological examination, revealed extensive damage to the fascular system which resulted in widespread haemorrhages.", "contents": "Onyalai--the possible involvement of a mycotoxin produced by Phoma sorghina in the aetiology. A number of species of fungi were isolated from millet (Pennisetum typhoides Staph and Hubb) and grain sorghum (Sorghum vulgare Pers) obtained from the households of patients suffering from onyalai. Several proved to be toxic to rats and chickens, including Phoma sorghina (Sacc.) which was the predominating fungus. Cultures of P. sorghina grown on maize and wheat were added to standard rations at concentrations of 5-30%. When fed to day-old New Hampshire chickens, the majority died within 4 days, exhibiting traces of blood on the beak and the cloaca. Rats given 10% or more of the mouldered material in the diet developed thrombocytopenia after 14 days which was followed by haematuria, epistaxis, melaena, and death. Pathological, including histological examination, revealed extensive damage to the fascular system which resulted in widespread haemorrhages."}
{"id": "PMID:1080887", "title": "The radiological presentation of pulmonary tuberculosis in children.", "content": "The children in this study had evidence of severe pulmonary disease when they were first radiographed. More than one segment, lobe or even lung was frequently involved and more than one type of lesion was often present in a patient. The right lung was more often affected. Segmental consolidation was common. Cavitary disease was particularly evident in children less than 3 years of age and many had extrapulmonary dissemination. Pleural effusions, calcification, and the acinar opacities of bronchogenic spread, were common in older children.", "contents": "The radiological presentation of pulmonary tuberculosis in children. The children in this study had evidence of severe pulmonary disease when they were first radiographed. More than one segment, lobe or even lung was frequently involved and more than one type of lesion was often present in a patient. The right lung was more often affected. Segmental consolidation was common. Cavitary disease was particularly evident in children less than 3 years of age and many had extrapulmonary dissemination. Pleural effusions, calcification, and the acinar opacities of bronchogenic spread, were common in older children."}
{"id": "PMID:1080888", "title": "Tuberculosis notwithstanding BCG vaccination.", "content": "In a series of 176 patients, 40 children (23%) developed tuberculosis notwithstanding BCG vaccination. The type and severity of the disease in this group were similar to those of the larger series of which this group was a part. However, BCG vaccination appeared to diminish the incidence of dissemination, especially tuberculous meningitis, and the number of deaths. The 40% incidence of tuberculous contacts probably indicates heavy exposure to infection. BCG offers a modicum of protection which the vulnerable should not be denied, but its use should not lead to complacency--the potency of vaccines and the technique of vaccination require constant surveillance. Case finding remains a priority and even BCG-vaccinated patients should be treated for tuberculosis when this is suspected.", "contents": "Tuberculosis notwithstanding BCG vaccination. In a series of 176 patients, 40 children (23%) developed tuberculosis notwithstanding BCG vaccination. The type and severity of the disease in this group were similar to those of the larger series of which this group was a part. However, BCG vaccination appeared to diminish the incidence of dissemination, especially tuberculous meningitis, and the number of deaths. The 40% incidence of tuberculous contacts probably indicates heavy exposure to infection. BCG offers a modicum of protection which the vulnerable should not be denied, but its use should not lead to complacency--the potency of vaccines and the technique of vaccination require constant surveillance. Case finding remains a priority and even BCG-vaccinated patients should be treated for tuberculosis when this is suspected."}
{"id": "PMID:1080890", "title": "Therapeutic transluminal arterial embolization for bleeding in the upper part of the gastrointestinal tract.", "content": "Diagnosis and the treatment of gastrointestinal bleeding by selective and superselective technique and the perfusion of vasopressors have been noted to have significant success. Transluminal embolization of a bleeding site represents an alternative method in patients who represent a poor operative risk and in those in whom vasopressor therapy has failed. The potential risks include intestinal ischemia and vary with the anatomic site.", "contents": "Therapeutic transluminal arterial embolization for bleeding in the upper part of the gastrointestinal tract. Diagnosis and the treatment of gastrointestinal bleeding by selective and superselective technique and the perfusion of vasopressors have been noted to have significant success. Transluminal embolization of a bleeding site represents an alternative method in patients who represent a poor operative risk and in those in whom vasopressor therapy has failed. The potential risks include intestinal ischemia and vary with the anatomic site."}
{"id": "PMID:1080891", "title": "Transcutaneous nerve stimulation for treatment of pain in patients with spinal cord injury.", "content": "Results of transcutaneous electrical nerve stimulation in 31 patients with spinal cord injury are presented. Success and failure are tabulated according to injury location and for type of pain perceived. Results indicate that pain at the site of injury is treated more successfully than radiating or central pain.", "contents": "Transcutaneous nerve stimulation for treatment of pain in patients with spinal cord injury. Results of transcutaneous electrical nerve stimulation in 31 patients with spinal cord injury are presented. Success and failure are tabulated according to injury location and for type of pain perceived. Results indicate that pain at the site of injury is treated more successfully than radiating or central pain."}
{"id": "PMID:1080892", "title": "Transcutaneous and peripheral nerve stimulation for chronic pain states.", "content": "A series of 120 patients with pain syndromes of varying sources were subjected to peripheral nerve electrical stimulation. Transcutaneous, percutaneous and depth electrode stimulation methods were employed. Thirty-eight patients obtained definite relief and twenty obtained equivocal relief. The remaining 62 patients obtained no relief. Pain sources are correlated with treatment results.", "contents": "Transcutaneous and peripheral nerve stimulation for chronic pain states. A series of 120 patients with pain syndromes of varying sources were subjected to peripheral nerve electrical stimulation. Transcutaneous, percutaneous and depth electrode stimulation methods were employed. Thirty-eight patients obtained definite relief and twenty obtained equivocal relief. The remaining 62 patients obtained no relief. Pain sources are correlated with treatment results."}
{"id": "PMID:1080893", "title": "Pain suppression by peripheral nerve stimulation. Part I. Observations with transcutaneous stimuli.", "content": "A systematic, strict appraisal was made of 100 patients, after preliminary clinical trials suggested that some patients with pain could be helped by peripheral nerve stimulation. Transcutaneous stimulation of different nerve trunks was done with a special electrical stimulation device with various selected electrical parameters. More than half of the patients experienced some relief; in many, this effect was obtained by stimulating nerves distant from the area of referred pain. Pain relief lasted for varying periods after stimulation. The maximum benefit was noticed after certain specific parameters were reached for each patient. A few patients had response decay, gain or worsening. Results differ to some degree from previous reports. The results seem encouraging for the treatment of certain forms of intractable pain.", "contents": "Pain suppression by peripheral nerve stimulation. Part I. Observations with transcutaneous stimuli. A systematic, strict appraisal was made of 100 patients, after preliminary clinical trials suggested that some patients with pain could be helped by peripheral nerve stimulation. Transcutaneous stimulation of different nerve trunks was done with a special electrical stimulation device with various selected electrical parameters. More than half of the patients experienced some relief; in many, this effect was obtained by stimulating nerves distant from the area of referred pain. Pain relief lasted for varying periods after stimulation. The maximum benefit was noticed after certain specific parameters were reached for each patient. A few patients had response decay, gain or worsening. Results differ to some degree from previous reports. The results seem encouraging for the treatment of certain forms of intractable pain."}
{"id": "PMID:1080894", "title": "Pain suppression by peripheral nerve stimulation. Part II. Observations with implanted devices.", "content": "Twenty-three patients underwent implantation of a stimulator system and were followed for 6-20 months. Twenty patients estimated between 50% and 100% pain relief. The effect was largely consistent and reproducible. Reduced drug intake and improved social performance were associated with subjective improvement. The surgical technique is given, complications are analyzed and parameters are discussed.", "contents": "Pain suppression by peripheral nerve stimulation. Part II. Observations with implanted devices. Twenty-three patients underwent implantation of a stimulator system and were followed for 6-20 months. Twenty patients estimated between 50% and 100% pain relief. The effect was largely consistent and reproducible. Reduced drug intake and improved social performance were associated with subjective improvement. The surgical technique is given, complications are analyzed and parameters are discussed."}
{"id": "PMID:1080895", "title": "Electrical stimulation of the nervous system.", "content": "Twenty-seven patients with chronic pain had dorsal column stimulators implanted for pain control over a period of three years. Technical factors of electrode placement and type of unit were not found to correlate with the degree of pain relief. The preoperative psychological attitude of the patient correlated highly with the degree of pain relief and was the single most important factor in patient selection and evaluation. Theoretical concepts of pain psychophysiology in relation to the gate theory and areas for more extensive investigation of pain are discussed.", "contents": "Electrical stimulation of the nervous system. Twenty-seven patients with chronic pain had dorsal column stimulators implanted for pain control over a period of three years. Technical factors of electrode placement and type of unit were not found to correlate with the degree of pain relief. The preoperative psychological attitude of the patient correlated highly with the degree of pain relief and was the single most important factor in patient selection and evaluation. Theoretical concepts of pain psychophysiology in relation to the gate theory and areas for more extensive investigation of pain are discussed."}
{"id": "PMID:1080897", "title": "Dorsal column stimulation: optimization of application.", "content": "Results are reviewed of dorsal column electrical stimulation for chronic pain in a series of 80 patients. In the light of the experience gained from this series, indications and patient suitability for this form of therapy are discussed. Complications occurring after implantation are tabulated.", "contents": "Dorsal column stimulation: optimization of application. Results are reviewed of dorsal column electrical stimulation for chronic pain in a series of 80 patients. In the light of the experience gained from this series, indications and patient suitability for this form of therapy are discussed. Complications occurring after implantation are tabulated."}
{"id": "PMID:1080898", "title": "Dorsal column stimulation for control of pain: a three-year follow-up.", "content": "The long-term (three-year) effect of dorsal column electrical stimulation for chronic pain in a group of 30 patients was generally decreased effectiveness with time, and increased stimulation thresholds. Further animal and human studies of this technique are recommended.", "contents": "Dorsal column stimulation for control of pain: a three-year follow-up. The long-term (three-year) effect of dorsal column electrical stimulation for chronic pain in a group of 30 patients was generally decreased effectiveness with time, and increased stimulation thresholds. Further animal and human studies of this technique are recommended."}
{"id": "PMID:1080899", "title": "Experience with dorsal column stimulation for relief of chronic intractable pain: 1968-1973.", "content": "Dorsal column stimulators (DCS) have been implanted in 130 patients with various chronic pain syndromes at the University of California, San Francisco, between 1969 and 1973. Preoperative psychiatric evaluation and percutaneous dorsal column stimulation testing were of value in rejecting those patients most likely to have unsatisfactory long-term results with DCS. Best results occurred in patients with phantom limb or peripheral nerve pain and worst results in patients with paraplegic pain, documented arachnoiditis, pancreatitis and arthritis. The need is stressed for careful preoperative selection and for close, prolonged postoperative care in a situation permitting access to multidisciplinary facilities for patient care.", "contents": "Experience with dorsal column stimulation for relief of chronic intractable pain: 1968-1973. Dorsal column stimulators (DCS) have been implanted in 130 patients with various chronic pain syndromes at the University of California, San Francisco, between 1969 and 1973. Preoperative psychiatric evaluation and percutaneous dorsal column stimulation testing were of value in rejecting those patients most likely to have unsatisfactory long-term results with DCS. Best results occurred in patients with phantom limb or peripheral nerve pain and worst results in patients with paraplegic pain, documented arachnoiditis, pancreatitis and arthritis. The need is stressed for careful preoperative selection and for close, prolonged postoperative care in a situation permitting access to multidisciplinary facilities for patient care."}
{"id": "PMID:1080900", "title": "Stimulation of the dorsal spinal cord for treatment of intractable pain: a preliminary report.", "content": "Electrical stimulation of the nervous system has been advocated as a means of alleviating pain in situations in which more conventional methods have been ineffective. A chronically implanted electrode on the dorsal surface of the spinal cord may prove to be a valuable adjunct to the neurosurgeon's armamentarium for pain control in selected individuals. The physiologic basis for this action is unclear but has been related to Melzack and Wall's gate control theory. This preliminary report deals with a series of patients treated with implanted dorsal cord stimulators.", "contents": "Stimulation of the dorsal spinal cord for treatment of intractable pain: a preliminary report. Electrical stimulation of the nervous system has been advocated as a means of alleviating pain in situations in which more conventional methods have been ineffective. A chronically implanted electrode on the dorsal surface of the spinal cord may prove to be a valuable adjunct to the neurosurgeon's armamentarium for pain control in selected individuals. The physiologic basis for this action is unclear but has been related to Melzack and Wall's gate control theory. This preliminary report deals with a series of patients treated with implanted dorsal cord stimulators."}
{"id": "PMID:1080901", "title": "Dorsal column stimulation and its prospects.", "content": "Results of a series of dorsal column stimulation implants in 76 patients are tabulated and correlated to implant location, type of electrodes used and pain causes; complications are also discussed.", "contents": "Dorsal column stimulation and its prospects. Results of a series of dorsal column stimulation implants in 76 patients are tabulated and correlated to implant location, type of electrodes used and pain causes; complications are also discussed."}
{"id": "PMID:1080902", "title": "Electrical stimulation of the nervous system for control of pain: University of Texas Southwestern Medical School experience.", "content": "A number of electrical stimulation procedures on patients with chronic pain were carried out at the University of Texas Southwestern Medical School. The procedures included dorsal column stimulation, peripheral nerve stimulation by means of implants and transcutaneous nerve stimulation. Some of the results are discussed and tabulated.", "contents": "Electrical stimulation of the nervous system for control of pain: University of Texas Southwestern Medical School experience. A number of electrical stimulation procedures on patients with chronic pain were carried out at the University of Texas Southwestern Medical School. The procedures included dorsal column stimulation, peripheral nerve stimulation by means of implants and transcutaneous nerve stimulation. Some of the results are discussed and tabulated."}
{"id": "PMID:1080903", "title": "Spinal cord stimulation in post-amputation pain.", "content": "One of the pre-operative screening tests for dorsal column stimulation involves direct acute precutaneous stimulation of the dorsal column. The test simulates the postoperative therapeutic situation, shows the patient's reactions to electrically induced paresthesias and enables physician and patient to evaluate beforehand the degree of pain relief to be obtained with the implant.", "contents": "Spinal cord stimulation in post-amputation pain. One of the pre-operative screening tests for dorsal column stimulation involves direct acute precutaneous stimulation of the dorsal column. The test simulates the postoperative therapeutic situation, shows the patient's reactions to electrically induced paresthesias and enables physician and patient to evaluate beforehand the degree of pain relief to be obtained with the implant."}
{"id": "PMID:1080904", "title": "Dorsal column stimulation: optimization of application.", "content": "Although the basic efficacy of dorsal column stimulation (DCS) has been shown, its optimal use remains to be defined. Since 1970, a program designed to maximize clinical success with DCS has been under way at Temple University Health Sciences Center. This article reviews patients screening, operative technique, new instrumentation and postoperative adjustment in DCS. Clinical results and long-term impressions of 90 implants in 75 patients are reported.", "contents": "Dorsal column stimulation: optimization of application. Although the basic efficacy of dorsal column stimulation (DCS) has been shown, its optimal use remains to be defined. Since 1970, a program designed to maximize clinical success with DCS has been under way at Temple University Health Sciences Center. This article reviews patients screening, operative technique, new instrumentation and postoperative adjustment in DCS. Clinical results and long-term impressions of 90 implants in 75 patients are reported."}
{"id": "PMID:1080905", "title": "Percutaneous implantation of chronic spinal cord electrodes for control of intractable pain: preliminary report.", "content": "A percutaneous technique is described for implanting a dorsal or ventral column stimulator for pain control with minimal surgical trauma. If the patient does not respond to percutaneous on-surface spinal stimulation, a percutaneous cordotomy can be carried out, as the needle is already in the correct position. Stimulation of the cord at the C1-2 level can be used for treatment of trigeminal neuralgia.", "contents": "Percutaneous implantation of chronic spinal cord electrodes for control of intractable pain: preliminary report. A percutaneous technique is described for implanting a dorsal or ventral column stimulator for pain control with minimal surgical trauma. If the patient does not respond to percutaneous on-surface spinal stimulation, a percutaneous cordotomy can be carried out, as the needle is already in the correct position. Stimulation of the cord at the C1-2 level can be used for treatment of trigeminal neuralgia."}
{"id": "PMID:1080906", "title": "Evaluation of pain relief.", "content": "Obtaining objective data on pain-relieving procedures is a simple matter. Measures of pain, activity levels and analgesic intake can be collected not only in a hospital but also in an outpatient setting. The data obtained make possible assessment of the effectiveness of a technique in any particular patient, and when combined for groups of patients, such data facilitate comparison of the effectiveness of different techniques in different pain states.", "contents": "Evaluation of pain relief. Obtaining objective data on pain-relieving procedures is a simple matter. Measures of pain, activity levels and analgesic intake can be collected not only in a hospital but also in an outpatient setting. The data obtained make possible assessment of the effectiveness of a technique in any particular patient, and when combined for groups of patients, such data facilitate comparison of the effectiveness of different techniques in different pain states."}
{"id": "PMID:1080907", "title": "Chronic thalamic and internal capsule stimulation for the control of central pain.", "content": "Thalamic electrical stimulation by means of chemically implanted electrodes and an externally placed transmitter was effected in patients with central pain. Good response was obtained in four patients with anesthesia dolorosa, four with thalamic syndrome, and one with paraplegic pain. Two patients with medullary syndrome experienced no relief. The mechanism of thalamic stimulation in the relief of pain is discussed and further clinical trials are anticipated.", "contents": "Chronic thalamic and internal capsule stimulation for the control of central pain. Thalamic electrical stimulation by means of chemically implanted electrodes and an externally placed transmitter was effected in patients with central pain. Good response was obtained in four patients with anesthesia dolorosa, four with thalamic syndrome, and one with paraplegic pain. Two patients with medullary syndrome experienced no relief. The mechanism of thalamic stimulation in the relief of pain is discussed and further clinical trials are anticipated."}
{"id": "PMID:1080908", "title": "Intermittent stimulation of nucleus ventralis posterolateralis for intractable pain.", "content": "A number of patients with intractable pain were subjected to electrical stimulation of the nucleus ventralis posterolateralis. Positive results obtained support the theory according to which pain is caused by lack of proprioceptive stimuli reaching the thalamus. The implantation technique is discussed and improvements of electrodes and of the device are proposed. Optimal stimulation parameters are defined.", "contents": "Intermittent stimulation of nucleus ventralis posterolateralis for intractable pain. A number of patients with intractable pain were subjected to electrical stimulation of the nucleus ventralis posterolateralis. Positive results obtained support the theory according to which pain is caused by lack of proprioceptive stimuli reaching the thalamus. The implantation technique is discussed and improvements of electrodes and of the device are proposed. Optimal stimulation parameters are defined."}
{"id": "PMID:1080909", "title": "Transcutaneous electrical stimulation for treatment of chronic pain: a preliminary report.", "content": "Two hundred thirty patients received transcutaneous electrical stimulation for intractable pain. Although almost 60% of the patients noted some degree of pain relief, slightly less than half (43%) of those tested decided to purchase a stimulator. About 20% of those who bought stimulators did not continue to obtain pain relief from transcutaneous electrical stimulation during the two to six month follow-up period. Many others noted a decrease in the amount of pain relief they obtained.", "contents": "Transcutaneous electrical stimulation for treatment of chronic pain: a preliminary report. Two hundred thirty patients received transcutaneous electrical stimulation for intractable pain. Although almost 60% of the patients noted some degree of pain relief, slightly less than half (43%) of those tested decided to purchase a stimulator. About 20% of those who bought stimulators did not continue to obtain pain relief from transcutaneous electrical stimulation during the two to six month follow-up period. Many others noted a decrease in the amount of pain relief they obtained."}
{"id": "PMID:1080910", "title": "Distal coronary artery bypass: local occlusion vs. ischemic arrest.", "content": "A retrospective study was performed on 65 patients with intractable angina and subtotal coronary occlusions undergoing aortocoronary artery bypass (ACB) to determine whether the local occlusion (L.O.) or the ischemic arrest (I.A.) technique was perferable in performing the distal coronary artery bypass. In 23 patients who had the method of local occlusion, a 45.7 percent incidence of myocardial injury was observed, compared to 42 patients undergoing ACB with ischemic arrest in whom an incidence of 22.5 percent occurred. Both serum glutamic oxaloacetic transaminase (SGOT) and creatine phosphokinase activity (CPK) determinations at 24 hours following operation in those patients not demonstrating evidence of postoperative injury were statistically higher in the local occlusion group compared to the ischemic arrest group. In the L.O. group SGOT was 155 I.U. compared to 77 I.U. in the I.A. group (p less than 0.01). CPK was 72 I.U. in the L.O. groupcompared to 34 I.U. in the I.A. group (p less than 0.05). These data suggest that, in subtotal coronary artery obstruction, the use of the L.O. technique is associated with a higher incidence of myocardial injury than is the technique of total cardiac I.A.", "contents": "Distal coronary artery bypass: local occlusion vs. ischemic arrest. A retrospective study was performed on 65 patients with intractable angina and subtotal coronary occlusions undergoing aortocoronary artery bypass (ACB) to determine whether the local occlusion (L.O.) or the ischemic arrest (I.A.) technique was perferable in performing the distal coronary artery bypass. In 23 patients who had the method of local occlusion, a 45.7 percent incidence of myocardial injury was observed, compared to 42 patients undergoing ACB with ischemic arrest in whom an incidence of 22.5 percent occurred. Both serum glutamic oxaloacetic transaminase (SGOT) and creatine phosphokinase activity (CPK) determinations at 24 hours following operation in those patients not demonstrating evidence of postoperative injury were statistically higher in the local occlusion group compared to the ischemic arrest group. In the L.O. group SGOT was 155 I.U. compared to 77 I.U. in the I.A. group (p less than 0.01). CPK was 72 I.U. in the L.O. groupcompared to 34 I.U. in the I.A. group (p less than 0.05). These data suggest that, in subtotal coronary artery obstruction, the use of the L.O. technique is associated with a higher incidence of myocardial injury than is the technique of total cardiac I.A."}
{"id": "PMID:1080912", "title": "Further investigations on the radioprotective effectiveness of tianabol and other drugs in fractionatedly irradiated rats.", "content": "Dianabol administered p.o. in the dose of 1.0 mg/kg during 10 days before irradiation-had no radioprotective effect in the fractionated irradiation (5 x 150 R). Distinct radioprotection, however, was exerted by the DMSO, Trasylol and Trichlorfon (30 mg/kg) administration.", "contents": "Further investigations on the radioprotective effectiveness of tianabol and other drugs in fractionatedly irradiated rats. Dianabol administered p.o. in the dose of 1.0 mg/kg during 10 days before irradiation-had no radioprotective effect in the fractionated irradiation (5 x 150 R). Distinct radioprotection, however, was exerted by the DMSO, Trasylol and Trichlorfon (30 mg/kg) administration."}
{"id": "PMID:1080913", "title": "Infection of the mosquito Aedes aegypti with infectious West Nile virus-antibody complexes.", "content": "Aedes aegypti fed through chick skin membranes on West Nile virus-homologous antiserum mixtures shown by an anti-globulin neutralization test to be highly infectious complexes (in terms of plaque formation in tissue culture) failed to become infected. Control mosquitoes fed on West Nile virus--normal rabbit serum mixtures containing similar or smaller amounts of infectious virus were shown to become infected. Mosquitoes ingesting suspensions of West Nile virus previously incubated with Murray Valley encephalitis or Ntaya antiserum became infected at significantly lower rates (P = less than 0-05) than controls ingesting West Nile virus-normal rabbit serum mixtures. West Nile virus--17D yellow fever or dengue--2 antiserum mixtures did not produce significantly reduced infection rates in Ae. aegypti when compared to controls.", "contents": "Infection of the mosquito Aedes aegypti with infectious West Nile virus-antibody complexes. Aedes aegypti fed through chick skin membranes on West Nile virus-homologous antiserum mixtures shown by an anti-globulin neutralization test to be highly infectious complexes (in terms of plaque formation in tissue culture) failed to become infected. Control mosquitoes fed on West Nile virus--normal rabbit serum mixtures containing similar or smaller amounts of infectious virus were shown to become infected. Mosquitoes ingesting suspensions of West Nile virus previously incubated with Murray Valley encephalitis or Ntaya antiserum became infected at significantly lower rates (P = less than 0-05) than controls ingesting West Nile virus-normal rabbit serum mixtures. West Nile virus--17D yellow fever or dengue--2 antiserum mixtures did not produce significantly reduced infection rates in Ae. aegypti when compared to controls."}
{"id": "PMID:1080917", "title": "[Nuclear structures in the late oogenesis of the common frog. I. Light microscopic observations].", "content": "The dynamics of the Rana temporaria karyosphere formation throughout different seasons was studied in addition to morphological changes in the karyosphere components with and without hormonal stimulation of oocyte maturation in vitro. Towards the end of summer, the nuclear structure (lampbrush chromosomes and multiple nucleoli) were shown to assemble in the centre of the nucleus forming a karyosphere. The completely formed karyosphere (up to 150--200 mu in diameter) is observed during autumn and winter to consist of the central chromosome part and a surrounding capsule. The capsule consists of numerous nucleoli and a distinct fibrous component. Filaments penetrating the whole nucleolar zone of the capsule are tightly packed around the chromosomes, thus forming the inner fibrous zone of the capsule, separating the chromosomes from the nucleoli. On the periphery of the karyosphere, threads attached to the nucleoli are clearly seen. This pattern of the karyosphere construction is conserved through winter till spring. In winter, spherical nucleoli change into rings and necklaces. In spring, nucleoli become spherical again and lose their threads. In the period of oocyte maturation and disappearance of nuclear envelopes, numerous nucleoli fuse into a single conglomeration which later disappears. Only the inner fibrous zone is conserved which separates the chromosomes from the cytoplasm and yolk platelets. In this state, the karyosphere persists up to the first meiotic metaphase. The nature of the fibrous component of the karyosphere capsule, its origin and function are discussed.", "contents": "[Nuclear structures in the late oogenesis of the common frog. I. Light microscopic observations]. The dynamics of the Rana temporaria karyosphere formation throughout different seasons was studied in addition to morphological changes in the karyosphere components with and without hormonal stimulation of oocyte maturation in vitro. Towards the end of summer, the nuclear structure (lampbrush chromosomes and multiple nucleoli) were shown to assemble in the centre of the nucleus forming a karyosphere. The completely formed karyosphere (up to 150--200 mu in diameter) is observed during autumn and winter to consist of the central chromosome part and a surrounding capsule. The capsule consists of numerous nucleoli and a distinct fibrous component. Filaments penetrating the whole nucleolar zone of the capsule are tightly packed around the chromosomes, thus forming the inner fibrous zone of the capsule, separating the chromosomes from the nucleoli. On the periphery of the karyosphere, threads attached to the nucleoli are clearly seen. This pattern of the karyosphere construction is conserved through winter till spring. In winter, spherical nucleoli change into rings and necklaces. In spring, nucleoli become spherical again and lose their threads. In the period of oocyte maturation and disappearance of nuclear envelopes, numerous nucleoli fuse into a single conglomeration which later disappears. Only the inner fibrous zone is conserved which separates the chromosomes from the cytoplasm and yolk platelets. In this state, the karyosphere persists up to the first meiotic metaphase. The nature of the fibrous component of the karyosphere capsule, its origin and function are discussed."}
{"id": "PMID:1080914", "title": "A technique for separation of canine lymphocytes and their use in the lymphocytotoxic, blastogenic, and rosette assys.", "content": "When the techniques established for preparing lymphocytes from human blood were applied to canine blood, the resulting preparations were contaminated with significant numbers of other blood cells. A three-step technique is described here for the separation of canine lymphocytes from blood which eliminates most granulocytes, platelets, and erythrocytes. Defibrinated blood is incubated with iron particles to allow phagocytosis by granulocytes. When mixed with Plasmagel, erythrocytes and iron-containing granulocytes sediment rapidly, leaving a lymphocyte-rich supernate plasma which is recovered and banded by centrifugation on a Ficoll-Hypaque gradient. Any erythrocytes remaining in the lymphocyte suspension aspirated from the gradient are eliminated by hypotonic lysis and centrifugation. The resulting preparation, representing 30 per cent recovery of the circulating lymphocytes, contained 85 to 94 per cent lymphocytes with a cell viability of 99 per cent. The functional capacity of the lymphocyte preparation was tested in three assays: 1) lymphoblastic transformation using phytohemagglutinin and pokeweed mitogen, 2) E rosette formation with human erythrocytes showed a mean 5 per cent rosette forming lymphocytes, 3) antibody-mediated lymphocytotoxicity using a canine anti-lymphocyte serum showed titers reproducible within one tube dilution.", "contents": "A technique for separation of canine lymphocytes and their use in the lymphocytotoxic, blastogenic, and rosette assys. When the techniques established for preparing lymphocytes from human blood were applied to canine blood, the resulting preparations were contaminated with significant numbers of other blood cells. A three-step technique is described here for the separation of canine lymphocytes from blood which eliminates most granulocytes, platelets, and erythrocytes. Defibrinated blood is incubated with iron particles to allow phagocytosis by granulocytes. When mixed with Plasmagel, erythrocytes and iron-containing granulocytes sediment rapidly, leaving a lymphocyte-rich supernate plasma which is recovered and banded by centrifugation on a Ficoll-Hypaque gradient. Any erythrocytes remaining in the lymphocyte suspension aspirated from the gradient are eliminated by hypotonic lysis and centrifugation. The resulting preparation, representing 30 per cent recovery of the circulating lymphocytes, contained 85 to 94 per cent lymphocytes with a cell viability of 99 per cent. The functional capacity of the lymphocyte preparation was tested in three assays: 1) lymphoblastic transformation using phytohemagglutinin and pokeweed mitogen, 2) E rosette formation with human erythrocytes showed a mean 5 per cent rosette forming lymphocytes, 3) antibody-mediated lymphocytotoxicity using a canine anti-lymphocyte serum showed titers reproducible within one tube dilution."}
{"id": "PMID:1080918", "title": "[Lymphocyte blast transformation in the mouse spleen under the influence of a low-molecular humoral thymus factor in vitro].", "content": "The mitogen activity of a low molecular lymphocytosis stimulating substance isolated from the thymus (LSS) has been investigated in cultured mouse CBA spleen cells. LSS caused transformation of lymphocytes to the blast cells capable of mitotic division. The initial signs of transformation were observed after 24 hours of incubation, reaching the maximum in 48 hour old cultures. The stimulating action of LSS held true for about 7 days. The intensity of blastogenesis depended on the dose of LSS. A high concentration LSS inhibited the blastogenic response. B-lymphocytes are not sensitive to LSS. It is concluded that the low molecular humoral factor of the thymus activated specifically the thymus-depended lymphocytes.", "contents": "[Lymphocyte blast transformation in the mouse spleen under the influence of a low-molecular humoral thymus factor in vitro]. The mitogen activity of a low molecular lymphocytosis stimulating substance isolated from the thymus (LSS) has been investigated in cultured mouse CBA spleen cells. LSS caused transformation of lymphocytes to the blast cells capable of mitotic division. The initial signs of transformation were observed after 24 hours of incubation, reaching the maximum in 48 hour old cultures. The stimulating action of LSS held true for about 7 days. The intensity of blastogenesis depended on the dose of LSS. A high concentration LSS inhibited the blastogenic response. B-lymphocytes are not sensitive to LSS. It is concluded that the low molecular humoral factor of the thymus activated specifically the thymus-depended lymphocytes."}
{"id": "PMID:1080923", "title": "Alpha-1-antitrypsin deficiency, mitochondrial antibodies and possible primary biliary cirrhosis. A case report and family study.", "content": "A case of a 70-year-old woman with a history of gastric ulcer and several pneumonias is presented. She was found to have pulmonary emphysema, severe alpha-1-antitrypsin (alpha1AT) deficiency and raised serum mitochondrial antibodies. Surgical liver biopsy showed portal liver cirrhosis, PAS-positive, diastaseresistant globules in the hepatocytes and changes interpreted as florid duct lesion of primary biliary cirrhosis. A brother has severe alpha1AT deficiency. Two daughters had raised mitochondrial antibodies. One of the latter had a granulomatous hepatitis, a common finding in primary biliary cirrhosis. The association of alpha1AT deficiency and primary biliary cirrhosis does not seem to have been described previously.", "contents": "Alpha-1-antitrypsin deficiency, mitochondrial antibodies and possible primary biliary cirrhosis. A case report and family study. A case of a 70-year-old woman with a history of gastric ulcer and several pneumonias is presented. She was found to have pulmonary emphysema, severe alpha-1-antitrypsin (alpha1AT) deficiency and raised serum mitochondrial antibodies. Surgical liver biopsy showed portal liver cirrhosis, PAS-positive, diastaseresistant globules in the hepatocytes and changes interpreted as florid duct lesion of primary biliary cirrhosis. A brother has severe alpha1AT deficiency. Two daughters had raised mitochondrial antibodies. One of the latter had a granulomatous hepatitis, a common finding in primary biliary cirrhosis. The association of alpha1AT deficiency and primary biliary cirrhosis does not seem to have been described previously."}
{"id": "PMID:1080924", "title": "The cause of hemifacial spasm: definitive microsurgical treatment at the brainstem in 31 patients.", "content": "Hemifacial spasm, like tic douloureux and several other cranial nerve dysfunction syndromes, is a symptom caused by cross compression-distortion, usually arterial, of the appropriate nerve at the brainstem. Symptoms of disordered hyperactivity and mild loss of function can be reversed by microsurgical mobilization of the vessel away from the nerve. This appears to be a safe and definitive procedure, but it should be performed only by a trained microneurosurgeon with experience in the cerebellopontine angle.", "contents": "The cause of hemifacial spasm: definitive microsurgical treatment at the brainstem in 31 patients. Hemifacial spasm, like tic douloureux and several other cranial nerve dysfunction syndromes, is a symptom caused by cross compression-distortion, usually arterial, of the appropriate nerve at the brainstem. Symptoms of disordered hyperactivity and mild loss of function can be reversed by microsurgical mobilization of the vessel away from the nerve. This appears to be a safe and definitive procedure, but it should be performed only by a trained microneurosurgeon with experience in the cerebellopontine angle."}
{"id": "PMID:1080934", "title": "[Immunological cell markers on lymphoblasts of patients with acid phosphatase positive acute leukemia (author's transl)].", "content": "Blast cells of 4 patients with acid phosphatase positive acute leukemia were investigated for T and B lymphocyte markers. Nearly all blast cells showed a typical T cell marker, namely spontaneous rosette formation with sheep red blood cells. No surface immunoglobulin was demonstrable on these cells. 3 of these 4 patients showed an enlargement of the upper mediastinum most probably due to the thymus. The conclusion is drawn that the acid phosphatase positive acute leukemia is a T cell leukemia. Some clinical data about these 4 patients are given.", "contents": "[Immunological cell markers on lymphoblasts of patients with acid phosphatase positive acute leukemia (author's transl)]. Blast cells of 4 patients with acid phosphatase positive acute leukemia were investigated for T and B lymphocyte markers. Nearly all blast cells showed a typical T cell marker, namely spontaneous rosette formation with sheep red blood cells. No surface immunoglobulin was demonstrable on these cells. 3 of these 4 patients showed an enlargement of the upper mediastinum most probably due to the thymus. The conclusion is drawn that the acid phosphatase positive acute leukemia is a T cell leukemia. Some clinical data about these 4 patients are given."}
{"id": "PMID:1080939", "title": "[Treatment of progressive muscular dystrophy].", "content": "The paper summarizes experience in treating 306 patients with progressive muscular dystrophy during different phases of the disease. The treatment was made with due consideration for a disturbed protein metabolism, a decreased permeability of the membrane in muscular filaments, a normalization of the metabolic product supply from the blood, improvement of the blood flow and cardio-vascular and respiratory insufficiency. The treatment was carried out in the form of a continuous use in in- and out-patient conditions of a session of anabolic hormones, vascular dilatative drugs, anticholinesterase preparations, amino acids, vitamins, drugs facilitating the cardio-vascular and respiratory activity. Besides, the patients were treated by oxygenobarotherapy, muscular electrostimulation by currents of sound frequencies, remedial gymnastics, massage, hypdrotherapy, etc. The authors propose typical treatment complexes. A follow-up study of patients who received a full course of therapy during three years demonstrated its effectiveness which was expressed by the absence of clinical signs of progressiveness.", "contents": "[Treatment of progressive muscular dystrophy]. The paper summarizes experience in treating 306 patients with progressive muscular dystrophy during different phases of the disease. The treatment was made with due consideration for a disturbed protein metabolism, a decreased permeability of the membrane in muscular filaments, a normalization of the metabolic product supply from the blood, improvement of the blood flow and cardio-vascular and respiratory insufficiency. The treatment was carried out in the form of a continuous use in in- and out-patient conditions of a session of anabolic hormones, vascular dilatative drugs, anticholinesterase preparations, amino acids, vitamins, drugs facilitating the cardio-vascular and respiratory activity. Besides, the patients were treated by oxygenobarotherapy, muscular electrostimulation by currents of sound frequencies, remedial gymnastics, massage, hypdrotherapy, etc. The authors propose typical treatment complexes. A follow-up study of patients who received a full course of therapy during three years demonstrated its effectiveness which was expressed by the absence of clinical signs of progressiveness."}
{"id": "PMID:1080940", "title": "[Influence of homologous n-alcanols on functional properties of isolated skeletal muscles IV. Action potential].", "content": "The influence of homologous n-alkyl alcohols (C2, C4, C8) on the membrane action potentials of isolated frog skeletal muscle fibres has been studied using the sucrose-gap method. 1. The alcanols tested effect a decrease in the height of the action potential and prolong the recovery phase of the spike. The threshold for excitation is increased. 2. The amplitude of these alcanol induced effects depends on the concentration of the alcanols, on the time of exposure and on the length of the hydrocarbon chain of the alcanols. 3. Membrane resistance, membrane resting potential, and membrane capacity are not affected by concentrations of alcanols which effect changes in the membrane action potential. 4. The findings are discussed in regard to alterations induced by alcanols in the conductance changes associated with a membrane action potential.", "contents": "[Influence of homologous n-alcanols on functional properties of isolated skeletal muscles IV. Action potential]. The influence of homologous n-alkyl alcohols (C2, C4, C8) on the membrane action potentials of isolated frog skeletal muscle fibres has been studied using the sucrose-gap method. 1. The alcanols tested effect a decrease in the height of the action potential and prolong the recovery phase of the spike. The threshold for excitation is increased. 2. The amplitude of these alcanol induced effects depends on the concentration of the alcanols, on the time of exposure and on the length of the hydrocarbon chain of the alcanols. 3. Membrane resistance, membrane resting potential, and membrane capacity are not affected by concentrations of alcanols which effect changes in the membrane action potential. 4. The findings are discussed in regard to alterations induced by alcanols in the conductance changes associated with a membrane action potential."}
{"id": "PMID:1080937", "title": "[DNA repair in murine lupus].", "content": "DNA repair was investigated in New Zealand Mice strains which develop murine lupus, and compared with Swiss control mice. Unscheduled DNA synthesis demonstrated by autoriadiography was used to measure the repair capacity of spleen cells. After gamma-irradiation DNA repair was decreased in the autoimmune strains, while it was significantly increased after UV-irradiation. A possible relationship between repair capacity after gamma-respectively UV-irradiation and the etiologic factor of autoimmunity is discussed.", "contents": "[DNA repair in murine lupus]. DNA repair was investigated in New Zealand Mice strains which develop murine lupus, and compared with Swiss control mice. Unscheduled DNA synthesis demonstrated by autoriadiography was used to measure the repair capacity of spleen cells. After gamma-irradiation DNA repair was decreased in the autoimmune strains, while it was significantly increased after UV-irradiation. A possible relationship between repair capacity after gamma-respectively UV-irradiation and the etiologic factor of autoimmunity is discussed."}
{"id": "PMID:1080941", "title": "Hemobilia: a case with recurrent jaundice cured by removal of a blood clot from the common bile duct.", "content": "A 78-year-old woman was admitted for recurrent jaundice, fever and biliary colic. At operation the common duct was found to be filled with a large blood clot. Removal of this clot resulted in a clinical cure.", "contents": "Hemobilia: a case with recurrent jaundice cured by removal of a blood clot from the common bile duct. A 78-year-old woman was admitted for recurrent jaundice, fever and biliary colic. At operation the common duct was found to be filled with a large blood clot. Removal of this clot resulted in a clinical cure."}
{"id": "PMID:1080942", "title": "Action of aprotinin on the survival of adult cerebellar neurons in organ culture.", "content": "Fragments of cerebellar cortex taken from adult rats were maintained for 7 days in organ culture. Inclusion in the medium of aprotinin, a polypeptide proteinase-inhibitor which also has carbohydrate-binding properties, was beneficial to the survival of neurons in the molecular layer, Purkinje cells and axons. This effect of aprotinin was mediated during the first 3 days in vitro. In a discussion of the various actions of aprotinin, it is concluded that the principal effect of the polypeptide on cultured central nervous tissue is to inhibit neuronal lysosomal proteinases activated by the trauma of explantation.", "contents": "Action of aprotinin on the survival of adult cerebellar neurons in organ culture. Fragments of cerebellar cortex taken from adult rats were maintained for 7 days in organ culture. Inclusion in the medium of aprotinin, a polypeptide proteinase-inhibitor which also has carbohydrate-binding properties, was beneficial to the survival of neurons in the molecular layer, Purkinje cells and axons. This effect of aprotinin was mediated during the first 3 days in vitro. In a discussion of the various actions of aprotinin, it is concluded that the principal effect of the polypeptide on cultured central nervous tissue is to inhibit neuronal lysosomal proteinases activated by the trauma of explantation."}
{"id": "PMID:1080943", "title": "Biasing of the summating potentials.", "content": "Summating potentials (SP) were recorded in the guinea pig cochlea, utilizing the differential-electrode technique. The effects of biasing the cochlear partition of the SP were observed under three different conditions: 1. electrical biasing--direct current polarization: 2. stimulus biasing--mechanical bias induced by low frequency sound stimulation, 3. COCB biasing--electrical stimulation of the crossed olivo-cochlear bundle. All three forms of biasing were found to have similar ellects on the SP which can be described in terms of the \"absolute direction of change\" in the potential. Under negative current (scala vestibuli negative; scala tympani positive), the negative phase of the stimulus bias (deflection of the cochlear partition towards scala vestibuli), or stimulation of the COCB, the SP is made more positive or less negative, as the case may be. In other words, the negative component of the differentially recorded SP is depressed, wheras the positive component is enhanced. The dominant influence os all three forms of biasing appears to be on mechanoelectric distortion arising in the hair-cell transduction process.", "contents": "Biasing of the summating potentials. Summating potentials (SP) were recorded in the guinea pig cochlea, utilizing the differential-electrode technique. The effects of biasing the cochlear partition of the SP were observed under three different conditions: 1. electrical biasing--direct current polarization: 2. stimulus biasing--mechanical bias induced by low frequency sound stimulation, 3. COCB biasing--electrical stimulation of the crossed olivo-cochlear bundle. All three forms of biasing were found to have similar ellects on the SP which can be described in terms of the \"absolute direction of change\" in the potential. Under negative current (scala vestibuli negative; scala tympani positive), the negative phase of the stimulus bias (deflection of the cochlear partition towards scala vestibuli), or stimulation of the COCB, the SP is made more positive or less negative, as the case may be. In other words, the negative component of the differentially recorded SP is depressed, wheras the positive component is enhanced. The dominant influence os all three forms of biasing appears to be on mechanoelectric distortion arising in the hair-cell transduction process."}
{"id": "PMID:1080946", "title": "Fine structure of granulocytes with cytoplasmic inclusions in pleural effusions from patients with rheumatoid pleuritis, tuberculous pleuritis and pleural carcinomatosis.", "content": "The fine structure of neutrophil granulocytes from pleural fluid obtained from patients with rheumatoid or tuberculous pleuritis or epidermoid carcinoma of the lung was identical irrespective of the primary disease of these patients. Homogenously stained lipid-like inclusions were present in the cytoplasm of the neutrophil granulocytes. They seemed to be formed de novo, as they lacked a distinct limiting unit membrane. The fine structure of the lipid-like inclusions gave no evidence to support the presence of immune complexes, these were probably located in the quantitative much fewer phagosomes of the granulocytes.", "contents": "Fine structure of granulocytes with cytoplasmic inclusions in pleural effusions from patients with rheumatoid pleuritis, tuberculous pleuritis and pleural carcinomatosis. The fine structure of neutrophil granulocytes from pleural fluid obtained from patients with rheumatoid or tuberculous pleuritis or epidermoid carcinoma of the lung was identical irrespective of the primary disease of these patients. Homogenously stained lipid-like inclusions were present in the cytoplasm of the neutrophil granulocytes. They seemed to be formed de novo, as they lacked a distinct limiting unit membrane. The fine structure of the lipid-like inclusions gave no evidence to support the presence of immune complexes, these were probably located in the quantitative much fewer phagosomes of the granulocytes."}
{"id": "PMID:1080945", "title": "Biological considerations for the use of homograft tympanic membranes and ossicles.", "content": "This report deals with some biological aspects of transplantation and preservation of tympanic membrane and ossicular grafts. The structure and behaviour of tympanic membrane grafts depends largely on the reaction of the preservatives with the tissue proteins. Due to these properties no observable change in the microscopical structure of the membranes and ossicles was found up to 3 years after alcohol and formaldehyde preservation. Preservation in cialit showed gradual disappearance of nuclei and an increasing disintegration of the fibrous structures. Irrespective of the mode of preservation, the fibrous tissue appeared to persist virtually unaffected for long periods. The monocellular reaction found around the grafts preserved in formaldehyde after heterolopie transplantation suggests that tissue antigens are not completely destroyed. A reaction to transplantation in the middle ear is only found around fresh heterologous grafts and around the fresh homologous grafts after previous sensitisation. As observed in rats, ossicles preserved in alcohol or cialit, obtained from revision operations in humans showed partial remodelling. Moreover in some cases these ossicular and especially the cartilaginous grafts showed areas of frank erosion.", "contents": "Biological considerations for the use of homograft tympanic membranes and ossicles. This report deals with some biological aspects of transplantation and preservation of tympanic membrane and ossicular grafts. The structure and behaviour of tympanic membrane grafts depends largely on the reaction of the preservatives with the tissue proteins. Due to these properties no observable change in the microscopical structure of the membranes and ossicles was found up to 3 years after alcohol and formaldehyde preservation. Preservation in cialit showed gradual disappearance of nuclei and an increasing disintegration of the fibrous structures. Irrespective of the mode of preservation, the fibrous tissue appeared to persist virtually unaffected for long periods. The monocellular reaction found around the grafts preserved in formaldehyde after heterolopie transplantation suggests that tissue antigens are not completely destroyed. A reaction to transplantation in the middle ear is only found around fresh heterologous grafts and around the fresh homologous grafts after previous sensitisation. As observed in rats, ossicles preserved in alcohol or cialit, obtained from revision operations in humans showed partial remodelling. Moreover in some cases these ossicular and especially the cartilaginous grafts showed areas of frank erosion."}
{"id": "PMID:1080949", "title": "Coronary artery bypass in patients over 70 years of age: indications and results.", "content": "With recent advances in all phases of coronary care and the increasing success of coronary arterial surgery, operative treatment of coronary artery disease is more readily recommended, even for patients over 70 years of age. In a series of 3,730 patients who underwent aortocoronary bypass from November 1969 through June 1974, there were 95 patients who were 70 years of age or older. The primary indication for surgery was severe angina, which was present in 88 patients. The mean coronary arterial score was 9.51. Associated valvular lesions were treated surgically in 21 patients, and a left ventricular aneurysm was resected in 7. Improvements in surgical technique and postoperative care are responsible for the hospital mortality rate of only 4.8 percent in the 21 patients operated upon during the first 6 months of 1974 compared with the overall mortality rate of 22.1 percent in all 95 patients. Long-term follow-up among the 95 patients includes data from 33 patients: 9 patients whose condition improved, 21 who were asymptomatic and 1 \"coronary death\".", "contents": "Coronary artery bypass in patients over 70 years of age: indications and results. With recent advances in all phases of coronary care and the increasing success of coronary arterial surgery, operative treatment of coronary artery disease is more readily recommended, even for patients over 70 years of age. In a series of 3,730 patients who underwent aortocoronary bypass from November 1969 through June 1974, there were 95 patients who were 70 years of age or older. The primary indication for surgery was severe angina, which was present in 88 patients. The mean coronary arterial score was 9.51. Associated valvular lesions were treated surgically in 21 patients, and a left ventricular aneurysm was resected in 7. Improvements in surgical technique and postoperative care are responsible for the hospital mortality rate of only 4.8 percent in the 21 patients operated upon during the first 6 months of 1974 compared with the overall mortality rate of 22.1 percent in all 95 patients. Long-term follow-up among the 95 patients includes data from 33 patients: 9 patients whose condition improved, 21 who were asymptomatic and 1 \"coronary death\"."}
{"id": "PMID:1080950", "title": "Myocardial revascularization after acute infarction.", "content": "The purpose of this study was (1) to establish the maximal interval between the onset of ischemia and reperfusion that would permit a decrease in the size of infarction, and (2) to evaluate the relation between changes in infarct size and preservation of cardiac function. Studies were carried out in 19 dogs of which 13 had temporary (1 to 3 hours) occlusion of the left anterior descending coronary artery. The hospital course of 15 patients of whom 13 underwent myocardial revascularization within 8 hours of acute infarction was also reviewed. In dogs, the eventual pathologic infarct size was significantly reduced if reperfusion was performed within 2 hours of ischemia. After 2 hours of ischemia, the revascularized segment remained dyskinetic on angiographic assessment and cardiac function was depressed. After 3 hours of ischemia, in spite of a patent coronary artery, the extent of infarct and dykinesia was greater than during ligation of the left anterior descending coronary artery. In patients, small infarcts developed with revascularization performed more than 4 hours after infarction but with revascularization of the left anterior descending coronary artery the size of the dyskinetic area (as assessed with angiography) was similar to that in patients with a closed graft to the left anterior descending coronary artery but with a patent graft to its diagonal branch. In all patients after revascularization the extent of the left ventricular dyskinetic area was smaller and cardiac function was significantly better than in patient who did not receive revascularization for complete occlusion of the left anterior descending coronary artery. In spite of successful revascularization, electrocardiographic evidence of transmural infarction persisted postoperatively. It is concluded that reperfusion of an area of myocardium that has been ischemic for less than 2 hours in dogs or less than 4 hours in man may lead to a significant reduction in the extent of infarction as well as improvement in cardiac function. However, the revascularized area remains angiographically dyskinetic and electrocardiographically abnormal.", "contents": "Myocardial revascularization after acute infarction. The purpose of this study was (1) to establish the maximal interval between the onset of ischemia and reperfusion that would permit a decrease in the size of infarction, and (2) to evaluate the relation between changes in infarct size and preservation of cardiac function. Studies were carried out in 19 dogs of which 13 had temporary (1 to 3 hours) occlusion of the left anterior descending coronary artery. The hospital course of 15 patients of whom 13 underwent myocardial revascularization within 8 hours of acute infarction was also reviewed. In dogs, the eventual pathologic infarct size was significantly reduced if reperfusion was performed within 2 hours of ischemia. After 2 hours of ischemia, the revascularized segment remained dyskinetic on angiographic assessment and cardiac function was depressed. After 3 hours of ischemia, in spite of a patent coronary artery, the extent of infarct and dykinesia was greater than during ligation of the left anterior descending coronary artery. In patients, small infarcts developed with revascularization performed more than 4 hours after infarction but with revascularization of the left anterior descending coronary artery the size of the dyskinetic area (as assessed with angiography) was similar to that in patients with a closed graft to the left anterior descending coronary artery but with a patent graft to its diagonal branch. In all patients after revascularization the extent of the left ventricular dyskinetic area was smaller and cardiac function was significantly better than in patient who did not receive revascularization for complete occlusion of the left anterior descending coronary artery. In spite of successful revascularization, electrocardiographic evidence of transmural infarction persisted postoperatively. It is concluded that reperfusion of an area of myocardium that has been ischemic for less than 2 hours in dogs or less than 4 hours in man may lead to a significant reduction in the extent of infarction as well as improvement in cardiac function. However, the revascularized area remains angiographically dyskinetic and electrocardiographically abnormal."}
{"id": "PMID:1080944", "title": "[Protein concentration of perilymph in guinea pigs after noise exposure].", "content": "The protein concentration in the perilymph of noise-exposed guinea pigs was investigated using a micromethod described previously. The animals were exposed to wide band noise at 140 dB for one hour unilaterally in a closed acoustic system. Perilymph was obtained after exposure at different intervals: immediately, 6 or 24 hours later. From animals exposed to noise, it is particularly more difficult to obtain perilymph without blood contamination than from normal ones. Even post mortem, it is difficult to obtain samples without blood contamination and without hemolysis, particularly from the scala tympani. Therefore, some animals were perfused intra-arterially with Ringer solution or with Infukoll M 40 before decapitation. After exposure to noise only a small increase in protein concentration of the perilymph can be detected in the noise-exposed ears of animals perfused intra-arterially. The difference in concentration is most distinct in samples extracted 6 hours after noise exposure had ceased. No reliable clue to the source of this protein could be obtained from these investigations.", "contents": "[Protein concentration of perilymph in guinea pigs after noise exposure]. The protein concentration in the perilymph of noise-exposed guinea pigs was investigated using a micromethod described previously. The animals were exposed to wide band noise at 140 dB for one hour unilaterally in a closed acoustic system. Perilymph was obtained after exposure at different intervals: immediately, 6 or 24 hours later. From animals exposed to noise, it is particularly more difficult to obtain perilymph without blood contamination than from normal ones. Even post mortem, it is difficult to obtain samples without blood contamination and without hemolysis, particularly from the scala tympani. Therefore, some animals were perfused intra-arterially with Ringer solution or with Infukoll M 40 before decapitation. After exposure to noise only a small increase in protein concentration of the perilymph can be detected in the noise-exposed ears of animals perfused intra-arterially. The difference in concentration is most distinct in samples extracted 6 hours after noise exposure had ceased. No reliable clue to the source of this protein could be obtained from these investigations."}
{"id": "PMID:1080952", "title": "A simple method to evaluate the massive dose vitamin A prophylaxis program in preschool children.", "content": "A massive dose vitamin A prophylaxis program is currently in operation in several States of India. Evaluation of this program on a large scale has so far been difficult due to lack of baseline data on the prevalence of vitamin A deficiency in children. In this study, a simple evaluation method which makes use of the linear relationship that exists between age on one hand and prevalence of ocular signs of vitamin A deficiency (Bitot's spots) on the other is described. Regression coefficients between age and prevalence of vitamin A deficiency of areas not covered by the program (control areas) were found to be significantly different both from zero and from regression coefficients of regularly covered areas (experimental areas). Also, all regression lines of control areas were found to be above the regression zone of experimental areas. These differences between the experimental and control areas which were significant, indicate the impact of the program in areas where the program is in operation. The regression coefficient calculated for one area where the program was discontinued after some time was found to be significantly different from zero, also it was different from the regression zone of the experimental areas. However, the regression coefficient of the discontinued area was found to be significantly different from the experimental areas only at 10% level, indicating a lesser degree of efficiency of the program in this area as compared to regularly fed areas. It is, therefore, suggested that evaluation of the massive dose prophylaxis program be done against the null hypothesis beta = 0.0065--the regression coefficient achievable under field conditions.", "contents": "A simple method to evaluate the massive dose vitamin A prophylaxis program in preschool children. A massive dose vitamin A prophylaxis program is currently in operation in several States of India. Evaluation of this program on a large scale has so far been difficult due to lack of baseline data on the prevalence of vitamin A deficiency in children. In this study, a simple evaluation method which makes use of the linear relationship that exists between age on one hand and prevalence of ocular signs of vitamin A deficiency (Bitot's spots) on the other is described. Regression coefficients between age and prevalence of vitamin A deficiency of areas not covered by the program (control areas) were found to be significantly different both from zero and from regression coefficients of regularly covered areas (experimental areas). Also, all regression lines of control areas were found to be above the regression zone of experimental areas. These differences between the experimental and control areas which were significant, indicate the impact of the program in areas where the program is in operation. The regression coefficient calculated for one area where the program was discontinued after some time was found to be significantly different from zero, also it was different from the regression zone of the experimental areas. However, the regression coefficient of the discontinued area was found to be significantly different from the experimental areas only at 10% level, indicating a lesser degree of efficiency of the program in this area as compared to regularly fed areas. It is, therefore, suggested that evaluation of the massive dose prophylaxis program be done against the null hypothesis beta = 0.0065--the regression coefficient achievable under field conditions."}
{"id": "PMID:1080953", "title": "Familial hypersensitivity pneumonitis.", "content": "Two families are described in which seven members of a total of 19 were found to have hypersensitivity pneumonitis due to exposure to avian antigens. Diagnosis was made on the basis of characteristic roentgenologic changes together with respiratory function and immunologic studies. The latter included screening for precipitins, macrophage migration inhibition (MMI) to specific antigens in avian serum and droppings, quantitation of immunoglobulin and alpha1 antitrypsin (AAT) levels and assessment of the complement system. Specific precipitins to pigeon and/or budgerigar serum were found in the serum of only four of the seven patients. Six of these seven patients, however, had a positive MMI. Thus, the MMI test, at least in this group of patients appeared to be a more sensitive indicator of active disease. The finding of seven members of two families with disease led to a search for predisposing factors, either genetic or environmental. Evidence for a genetic predisposition came from tissue typing studies. In the first family, both paternal haplotypes were associated with disease; the maternal haplotype HLA3,7 was not inherited by any child with disease. In the second family, the disease developed in three of four members with the haplotype HL-A2,W15, who were significantly exposed to avian antigen. In the light of recent studies showing an association between immune response (Ir) genes, histocompatibility antigens and disease susceptibility, these findings were interpreted as possible evidence for a subtle genetically linked immune defect in hypersensitivity pneumonitis. Evidence for an environmental predisposition was less clear cut, but it is interesting that members of both families used a gamma isomer of hexachlorobenzene (Nickoff) to eradicate mite infestations in their birds which might have damaged the bronchial mucosa or acted as an immunologic adjuvant in a person with underlying susceptibility to disease. The presence of subclinical respiratory disease in two family members is reported, and the importance of performing a range of investigations of respiratory function in order to detect disease and monitor its progress is emphasized.", "contents": "Familial hypersensitivity pneumonitis. Two families are described in which seven members of a total of 19 were found to have hypersensitivity pneumonitis due to exposure to avian antigens. Diagnosis was made on the basis of characteristic roentgenologic changes together with respiratory function and immunologic studies. The latter included screening for precipitins, macrophage migration inhibition (MMI) to specific antigens in avian serum and droppings, quantitation of immunoglobulin and alpha1 antitrypsin (AAT) levels and assessment of the complement system. Specific precipitins to pigeon and/or budgerigar serum were found in the serum of only four of the seven patients. Six of these seven patients, however, had a positive MMI. Thus, the MMI test, at least in this group of patients appeared to be a more sensitive indicator of active disease. The finding of seven members of two families with disease led to a search for predisposing factors, either genetic or environmental. Evidence for a genetic predisposition came from tissue typing studies. In the first family, both paternal haplotypes were associated with disease; the maternal haplotype HLA3,7 was not inherited by any child with disease. In the second family, the disease developed in three of four members with the haplotype HL-A2,W15, who were significantly exposed to avian antigen. In the light of recent studies showing an association between immune response (Ir) genes, histocompatibility antigens and disease susceptibility, these findings were interpreted as possible evidence for a subtle genetically linked immune defect in hypersensitivity pneumonitis. Evidence for an environmental predisposition was less clear cut, but it is interesting that members of both families used a gamma isomer of hexachlorobenzene (Nickoff) to eradicate mite infestations in their birds which might have damaged the bronchial mucosa or acted as an immunologic adjuvant in a person with underlying susceptibility to disease. The presence of subclinical respiratory disease in two family members is reported, and the importance of performing a range of investigations of respiratory function in order to detect disease and monitor its progress is emphasized."}
{"id": "PMID:1080954", "title": "The decreasing value of external cephalic version in modern obstetric practice.", "content": "The consequences of 1,308 attempts at external cephalic version in 866 patients over a 5 year period are reviewed. A fetal mortality rate of 0.9 per cent and an over-all complication rate of 4.4 per cent was found, and the value of attempting external cephalic version in modern obstetric practice is seriously questioned.", "contents": "The decreasing value of external cephalic version in modern obstetric practice. The consequences of 1,308 attempts at external cephalic version in 866 patients over a 5 year period are reviewed. A fetal mortality rate of 0.9 per cent and an over-all complication rate of 4.4 per cent was found, and the value of attempting external cephalic version in modern obstetric practice is seriously questioned."}
{"id": "PMID:1080955", "title": "Endothelial alterations in congenital corneal dystrophies.", "content": "We studied the clinical and ultrastructural findings in three different types of congenital endothelial dystrophies: hereditary posterior polymorphous dystrophy, congenital hereditary corneal dystrophy, and a nonhereditary congenital endothelial dystrophy. In the first patient, with hereditary posterior polymorphous dystrophy, a layer of epithelial-like cells was observed adjacent to endothelial cells on the posterior corneal surface. Descemet's membrane displayed a multilaminar pattern and consisted of an anterior, thin (3 mu), PAS-positive layer and a posterior, thicker (25 to 30 mu) zone of abnormal collagen. The second patient, with congenital hereditary endothelial dystrophy, showed a thickened multilaminar Descemet's membrane and scant endothelial cells. In the third patient with nonhereditary congenital endothelial dystrophy, the thickened Descemet's membrane was lined posteriorly by a retrocorneal fibrous membrane. A few degenerated endothelial cells were present. All three cases showed 100- to 110-nm banding posteriorly. In these three clinically distinct entities, electron microscopy was useful in demonstrating the unusual form of endothelial transformation to epithelial-like cells in one patient, in contrast to the more common fibroblast-like metaplasia of endothelial cells seen in the other two patients.", "contents": "Endothelial alterations in congenital corneal dystrophies. We studied the clinical and ultrastructural findings in three different types of congenital endothelial dystrophies: hereditary posterior polymorphous dystrophy, congenital hereditary corneal dystrophy, and a nonhereditary congenital endothelial dystrophy. In the first patient, with hereditary posterior polymorphous dystrophy, a layer of epithelial-like cells was observed adjacent to endothelial cells on the posterior corneal surface. Descemet's membrane displayed a multilaminar pattern and consisted of an anterior, thin (3 mu), PAS-positive layer and a posterior, thicker (25 to 30 mu) zone of abnormal collagen. The second patient, with congenital hereditary endothelial dystrophy, showed a thickened multilaminar Descemet's membrane and scant endothelial cells. In the third patient with nonhereditary congenital endothelial dystrophy, the thickened Descemet's membrane was lined posteriorly by a retrocorneal fibrous membrane. A few degenerated endothelial cells were present. All three cases showed 100- to 110-nm banding posteriorly. In these three clinically distinct entities, electron microscopy was useful in demonstrating the unusual form of endothelial transformation to epithelial-like cells in one patient, in contrast to the more common fibroblast-like metaplasia of endothelial cells seen in the other two patients."}
{"id": "PMID:1080956", "title": "Vitrectomy and intraocular gentamicin management of Herellea endophthalmitis after incomplete phacoemulsification.", "content": "A patient with Herellea endophthalmitis after incomplete phacoemulsification was treated with vitrectomy and intravitreal infusion of 8 mug/ml of gentamicin and 400 mug of dexamethasone. The vitrectomies were done through a pars plana incision with the vitrophage. Additionally, appropriate systemic antibiotics and prednisone were given.", "contents": "Vitrectomy and intraocular gentamicin management of Herellea endophthalmitis after incomplete phacoemulsification. A patient with Herellea endophthalmitis after incomplete phacoemulsification was treated with vitrectomy and intravitreal infusion of 8 mug/ml of gentamicin and 400 mug of dexamethasone. The vitrectomies were done through a pars plana incision with the vitrophage. Additionally, appropriate systemic antibiotics and prednisone were given."}
{"id": "PMID:1080957", "title": "The fine structure of thymoma, with emphasis on its differential diagnosis. A study of ten cases.", "content": "In this study, thymoma is defined as a neoplasm of the epithelial-reticular framework cells of the thymus. As in the normal thymus, these cells regularly displayed branching tonofilaments, macuale adherens, elongated processes, and basal lamina. These characteristics proved useful in the differential diagnosis of thymoma from a variety of anterior mediastinal tumors including thymic carcinoid, lymphoma, germinoma (seminoma type), and fibrous mesothelioma. Lymphocytes in the thymomas often showed mitotic activity and a moderate degree of transformation. The significance of this and the gland-like spaces, vacuolated epithelial cells, starry-sky appearance, emperipolesis, and perivascular spaces is discussed.", "contents": "The fine structure of thymoma, with emphasis on its differential diagnosis. A study of ten cases. In this study, thymoma is defined as a neoplasm of the epithelial-reticular framework cells of the thymus. As in the normal thymus, these cells regularly displayed branching tonofilaments, macuale adherens, elongated processes, and basal lamina. These characteristics proved useful in the differential diagnosis of thymoma from a variety of anterior mediastinal tumors including thymic carcinoid, lymphoma, germinoma (seminoma type), and fibrous mesothelioma. Lymphocytes in the thymomas often showed mitotic activity and a moderate degree of transformation. The significance of this and the gland-like spaces, vacuolated epithelial cells, starry-sky appearance, emperipolesis, and perivascular spaces is discussed."}
{"id": "PMID:1080959", "title": "Incidence of stress ulcer formation associated with steroid therapy in various shock states.", "content": "The use of pharmacologic doses of steroids early in the shock state, within the first hour when possible, for periods of less than forty-eight hours seems to reduce the incidence of gastric ulceration and hemorrhage in low flow states associated with septic, cardiogenic, and hemorrhagic shock.", "contents": "Incidence of stress ulcer formation associated with steroid therapy in various shock states. The use of pharmacologic doses of steroids early in the shock state, within the first hour when possible, for periods of less than forty-eight hours seems to reduce the incidence of gastric ulceration and hemorrhage in low flow states associated with septic, cardiogenic, and hemorrhagic shock."}
{"id": "PMID:1080960", "title": "T-lymphocyte levels in the peripheral blood of patients with cancer of the head and neck.", "content": "Percentage and levels of circulating T-lymphocytes were studied in forty-six patients with squamous cell carcinoma of the head and neck before and/or after treatment and compared with control values obtained in fifty-one healthy volunteers. Mean percentages of circulating T-cells and mean T-cell levels in cancer patients prior to treatment were significantly lower than control values. After surgery or radiation therapy the mean percentage of T-cells remained lower in comparison with that of the controls whereas T-cell levels in patients evaluated after surgical treatment increased in comparison with pretreatment values and were not significantly different from those of the controls.", "contents": "T-lymphocyte levels in the peripheral blood of patients with cancer of the head and neck. Percentage and levels of circulating T-lymphocytes were studied in forty-six patients with squamous cell carcinoma of the head and neck before and/or after treatment and compared with control values obtained in fifty-one healthy volunteers. Mean percentages of circulating T-cells and mean T-cell levels in cancer patients prior to treatment were significantly lower than control values. After surgery or radiation therapy the mean percentage of T-cells remained lower in comparison with that of the controls whereas T-cell levels in patients evaluated after surgical treatment increased in comparison with pretreatment values and were not significantly different from those of the controls."}
{"id": "PMID:1080961", "title": "High dose methotrexate as a preoperative adjuvant in the treatment of epidermoid carcinoma of the head and neck. A feasibility study and clinical trial.", "content": "Thirty patients with operable epidermoid carcinoma of the head and neck were treated with intravenous high dose methotrexate and leucovorin rescue prior to resection. Their clinical courses were compared with those of thirty randomly selected patients matched for tumors site and clinical stage who were treated by surgery alone. No medical or surgical complications associated with methotrexate were encountered. An objective decrease in tumor size (primary lesion or nodal metastases) was noted prior to resection in twenty-three patients (77 per cent). The number of recurrences in the two groups was similar. However, these was a significantly greater disease-free interval in the methotrexate-treated patients (p less than 0.05). No significant differences in survival have been noted to date between the two groups. In view of the absence of complications, the regressions in tumor size, and the increase in postoperative disease-free interval in this trial, evaluation as preoperative adjuvants of higher doses of methotrexate and of other chemotherapeutic agents in combination with methotrexate appears warranted.", "contents": "High dose methotrexate as a preoperative adjuvant in the treatment of epidermoid carcinoma of the head and neck. A feasibility study and clinical trial. Thirty patients with operable epidermoid carcinoma of the head and neck were treated with intravenous high dose methotrexate and leucovorin rescue prior to resection. Their clinical courses were compared with those of thirty randomly selected patients matched for tumors site and clinical stage who were treated by surgery alone. No medical or surgical complications associated with methotrexate were encountered. An objective decrease in tumor size (primary lesion or nodal metastases) was noted prior to resection in twenty-three patients (77 per cent). The number of recurrences in the two groups was similar. However, these was a significantly greater disease-free interval in the methotrexate-treated patients (p less than 0.05). No significant differences in survival have been noted to date between the two groups. In view of the absence of complications, the regressions in tumor size, and the increase in postoperative disease-free interval in this trial, evaluation as preoperative adjuvants of higher doses of methotrexate and of other chemotherapeutic agents in combination with methotrexate appears warranted."}
{"id": "PMID:1080962", "title": "[An equidensometric method for objective evaluation of ultrasonic b-scan pictures (author's transl)].", "content": "An equidensometric procedure is presented for achieving objective evaluation of ultrasonic B-scan pictures. Using special film (Agfacontour film) it is possible to select and show individually areas of equal brightness appearing on the B-scan, so called \"equidensites\". This can be done by colouring each area differently or by using a system of symbols. There is a description of the way in which the Agfacontour film works and is processed. Clinical evidence can be provided to prove the equidensometric evaluation of ultrasonic B-pictures of the eye can offer a considerable amount of additional information.", "contents": "[An equidensometric method for objective evaluation of ultrasonic b-scan pictures (author's transl)]. An equidensometric procedure is presented for achieving objective evaluation of ultrasonic B-scan pictures. Using special film (Agfacontour film) it is possible to select and show individually areas of equal brightness appearing on the B-scan, so called \"equidensites\". This can be done by colouring each area differently or by using a system of symbols. There is a description of the way in which the Agfacontour film works and is processed. Clinical evidence can be provided to prove the equidensometric evaluation of ultrasonic B-pictures of the eye can offer a considerable amount of additional information."}
{"id": "PMID:1080963", "title": "Ocular pulse wave in workers exposed to carbon disulfide.", "content": "Oculosphygmography (OSG), combined with electrocardiography, was performed on 38 male workers exposed occupationally to carbon disulfide (CS2) and 40 unexposed male paper mill workers. A statistical analysis of the results showed that the ocular pulse wave of the exposed group showed a significantly lower pattern than that of the unexposed group. A comparison between workers currently exposed to CS2 and those no longer exposed indicated that the hemodynamic alterations caused by CS2 exposure lead to a permanent condition. It is concluded that CS2 exposure increases the rigidity of the vascular bed of the eye. The data indicate that primarily chronic, subclinical CS2 poisoning causes capillary damage. Oculosphygmography seems to be one objective examination useful in diagnosing the subtle syndrome of chronic CS2 poisoning.", "contents": "Ocular pulse wave in workers exposed to carbon disulfide. Oculosphygmography (OSG), combined with electrocardiography, was performed on 38 male workers exposed occupationally to carbon disulfide (CS2) and 40 unexposed male paper mill workers. A statistical analysis of the results showed that the ocular pulse wave of the exposed group showed a significantly lower pattern than that of the unexposed group. A comparison between workers currently exposed to CS2 and those no longer exposed indicated that the hemodynamic alterations caused by CS2 exposure lead to a permanent condition. It is concluded that CS2 exposure increases the rigidity of the vascular bed of the eye. The data indicate that primarily chronic, subclinical CS2 poisoning causes capillary damage. Oculosphygmography seems to be one objective examination useful in diagnosing the subtle syndrome of chronic CS2 poisoning."}
{"id": "PMID:1080964", "title": "Management of dense secondary membranes with vitrophage.", "content": "Seven patients underwent a pars plana approach membranectomy and vitrectomy with the vitrophage for the removal of dense secondary membranes after planned or unplanned extracapsular lens extractions. In all cases the membranes and synechias were removed with restoration of clarity to the ocular media. In three cases the final vision was limited by unrelated ocular anomalies. There were no postoperative corneal or retinal complications. The advantages to the pars plana approach are the small incision, the prevention of vitreocorneal adhesion, and the ease with which the surgery is performed.", "contents": "Management of dense secondary membranes with vitrophage. Seven patients underwent a pars plana approach membranectomy and vitrectomy with the vitrophage for the removal of dense secondary membranes after planned or unplanned extracapsular lens extractions. In all cases the membranes and synechias were removed with restoration of clarity to the ocular media. In three cases the final vision was limited by unrelated ocular anomalies. There were no postoperative corneal or retinal complications. The advantages to the pars plana approach are the small incision, the prevention of vitreocorneal adhesion, and the ease with which the surgery is performed."}
{"id": "PMID:1080965", "title": "Methods of mathematical differentiation in tonography.", "content": "Our bulbar-compressure isotonographical method was applied to 28 normal and 40 glaucomatous eyes. This method was carried out twice on all patients using two pressure gradients: Pt=Po+10 and Pt=Po+30. Also, the outflow facility (C) was determined by Grant's tonography. Thus, 3 C-values were obtained with 3 varying pressure differences (Pt-Po=deltaP). With this differential tonography the mathematical determination and the graphic representation of a real C-value (Cdiff) by deltaP=O are possible. With normal eyes the average C-values were indirectly proportional to the pressure differences. This relationship was found to be directly proportional on glaucomatous eyes. The tonographical coefficients Po/Co-4, P3/CL3-7, Po-10/Co-4, and Po/Cintegral were calculated on 104 normal and 312 glaucomatous eyes. The results show small differences between the average values of the various coefficients for normal and glaucomatous eyes. The percentage of the pathological values on glaucomatous eyes varies from 57.4% to 67.3%. The diagnostic efficiency of the tonography is increased by the determination of Cdiff (percentage of positive values=70%) and the calculation of Po/Cdiff value (95%).", "contents": "Methods of mathematical differentiation in tonography. Our bulbar-compressure isotonographical method was applied to 28 normal and 40 glaucomatous eyes. This method was carried out twice on all patients using two pressure gradients: Pt=Po+10 and Pt=Po+30. Also, the outflow facility (C) was determined by Grant's tonography. Thus, 3 C-values were obtained with 3 varying pressure differences (Pt-Po=deltaP). With this differential tonography the mathematical determination and the graphic representation of a real C-value (Cdiff) by deltaP=O are possible. With normal eyes the average C-values were indirectly proportional to the pressure differences. This relationship was found to be directly proportional on glaucomatous eyes. The tonographical coefficients Po/Co-4, P3/CL3-7, Po-10/Co-4, and Po/Cintegral were calculated on 104 normal and 312 glaucomatous eyes. The results show small differences between the average values of the various coefficients for normal and glaucomatous eyes. The percentage of the pathological values on glaucomatous eyes varies from 57.4% to 67.3%. The diagnostic efficiency of the tonography is increased by the determination of Cdiff (percentage of positive values=70%) and the calculation of Po/Cdiff value (95%)."}
{"id": "PMID:1080966", "title": "[Typical retinitis pigmentosa with chronic progressive external ophthalmoplegia (author's transl)].", "content": "Report of a case of chronic progressive external ophthalmoplegia combined with retinitis pigmentosa, optic atrophy, marked attenuation of the retinal vessels, maculopathy, and complicated cataract. Visual fields and acuity were severely impaired. The ophthalmoplegia was histologically identified as ocular myopathy. In a review of 181 cases with CPEO  the disease was found to be associated with retinitis pigmentosa in 7 per cent and with atypical pigmentary retinopathy in 36 per cent of the cases. A distinction of these two types of retinal dystrophy is justified by the clinical features and may indicate different hereditary patterns.", "contents": "[Typical retinitis pigmentosa with chronic progressive external ophthalmoplegia (author's transl)]. Report of a case of chronic progressive external ophthalmoplegia combined with retinitis pigmentosa, optic atrophy, marked attenuation of the retinal vessels, maculopathy, and complicated cataract. Visual fields and acuity were severely impaired. The ophthalmoplegia was histologically identified as ocular myopathy. In a review of 181 cases with CPEO  the disease was found to be associated with retinitis pigmentosa in 7 per cent and with atypical pigmentary retinopathy in 36 per cent of the cases. A distinction of these two types of retinal dystrophy is justified by the clinical features and may indicate different hereditary patterns."}
{"id": "PMID:1080967", "title": "[The electrophysiological behavior of normal and glaucomatous human eyes with short term intraocular pressure elevation (author's transl)].", "content": "An electrophysiological study has been done on glaucomatous and normal eyes with briefly elevated intraocular pressure. There was no significant difference between the ERGs, but the changes in the VERs of glaucomatous eyes differed widely from those of normal eyes. This seems to be due to the high sensitivity to pressure of the prelaminary part of the optic nerve in glaucomatous eyes. Obviously this could be a method for the objective measurement of the sensitivity of the papillary vessels to raised intraocular pressure.", "contents": "[The electrophysiological behavior of normal and glaucomatous human eyes with short term intraocular pressure elevation (author's transl)]. An electrophysiological study has been done on glaucomatous and normal eyes with briefly elevated intraocular pressure. There was no significant difference between the ERGs, but the changes in the VERs of glaucomatous eyes differed widely from those of normal eyes. This seems to be due to the high sensitivity to pressure of the prelaminary part of the optic nerve in glaucomatous eyes. Obviously this could be a method for the objective measurement of the sensitivity of the papillary vessels to raised intraocular pressure."}
{"id": "PMID:1080968", "title": "[Peripheral retinal circulation times in connection with retinal detachment (author's transl)].", "content": "Concerning the pathogenesis of retinal detachment 4 groups of each 10 eyes have been examined by fluorescein angiography: the periphery of normal eyes (Table 2), ophthalmoscopically inconspicuous sectors of patients with detachment (Table 3), the area of lattice degeneration, radial perivascular degeneration and round holes without detachment (Table 4) and the area of tears and holes, when detachment has taken place (Table 5). The time passing between the injection of dye into the cubital vein and the first arterial filling in the retinal periphery is practically equal in all groups. The prolongation of retinal circulation time II from the first arterial to the complete venous filling in areas of lattice degeneration etc. without detachment seems to be in connection with small vessel closures. The striking short retinal circulation time I from the first arterial to the first venous filling in the cases of detachment is apparently caused by capillary dilatations central of the tears and holes; the dilated capillaries seem to have the function of a arteriovenous anastomosis. Own measurements of circulation times in the area of optic disc (Table 1), performed for comparisments, are accordant to literature.", "contents": "[Peripheral retinal circulation times in connection with retinal detachment (author's transl)]. Concerning the pathogenesis of retinal detachment 4 groups of each 10 eyes have been examined by fluorescein angiography: the periphery of normal eyes (Table 2), ophthalmoscopically inconspicuous sectors of patients with detachment (Table 3), the area of lattice degeneration, radial perivascular degeneration and round holes without detachment (Table 4) and the area of tears and holes, when detachment has taken place (Table 5). The time passing between the injection of dye into the cubital vein and the first arterial filling in the retinal periphery is practically equal in all groups. The prolongation of retinal circulation time II from the first arterial to the complete venous filling in areas of lattice degeneration etc. without detachment seems to be in connection with small vessel closures. The striking short retinal circulation time I from the first arterial to the first venous filling in the cases of detachment is apparently caused by capillary dilatations central of the tears and holes; the dilated capillaries seem to have the function of a arteriovenous anastomosis. Own measurements of circulation times in the area of optic disc (Table 1), performed for comparisments, are accordant to literature."}
{"id": "PMID:1080971", "title": "Life span of small lymphocytes in the thymolymphatic tissues of normal and thymus-deprived BALB/C mice.", "content": "Autoradiography and scintilation counting have been used after various schedules of 3H-thymidine injections to evaluate lymphocyte kinetics in normal and thymus-deprived BALB/C mice. The thymus was found to be an active production site of small lymphocytes, the majority of these cells having thymic residence times of three to five days. Peripheral lymphoid tissues--including spleen, lymph nodes and Peyer's patches--were dominated by long-lived lymphocytes and produced very few small lymphocytes. Nearly identical percentages of long-lived lymphocytes with comparable grain counts were found in the peripheral tissues. In adult thymectomized animals, and in mice thymectomized and reconstituted with bone marrow cells following total body irradiation, percentages and lifespan of long-lived lymphocytes were found to be of the same order as in normal or sham-operated controls. It is concluded that T cells in the peripheral long-lived pool can be formed outside the thymus and that the bone marrow probably produces long-lived B lymphocytes.", "contents": "Life span of small lymphocytes in the thymolymphatic tissues of normal and thymus-deprived BALB/C mice. Autoradiography and scintilation counting have been used after various schedules of 3H-thymidine injections to evaluate lymphocyte kinetics in normal and thymus-deprived BALB/C mice. The thymus was found to be an active production site of small lymphocytes, the majority of these cells having thymic residence times of three to five days. Peripheral lymphoid tissues--including spleen, lymph nodes and Peyer's patches--were dominated by long-lived lymphocytes and produced very few small lymphocytes. Nearly identical percentages of long-lived lymphocytes with comparable grain counts were found in the peripheral tissues. In adult thymectomized animals, and in mice thymectomized and reconstituted with bone marrow cells following total body irradiation, percentages and lifespan of long-lived lymphocytes were found to be of the same order as in normal or sham-operated controls. It is concluded that T cells in the peripheral long-lived pool can be formed outside the thymus and that the bone marrow probably produces long-lived B lymphocytes."}
{"id": "PMID:1080969", "title": "The use of protease inhibitor (trasylol) and heparin in cardiorespiratory resuscitation. I. Studies of the blood clotting system.", "content": "The purpose of the study was the assessment of the effect of protease inhibitor (trasylol) and heparin on the blood clotting system during cardiorespiratory resuscitation. The studies were carried out in two experimental groups of rabbits. In group I oxygen was given, cardiac massage was applied, alkalinizing agents and drugs improving the action of the heart and the peripheral circulation were administered. In group II trasylol and heparin were given additionally. The studies proved that cardiorespiratory arrest causes development of the intravascular clotting syndrome. Resuscitation management in group I failed to correct the disturbances in the blood clotting system. On the other hand, in group II they were partly corrected. The animals in this group survived the experiment. The administration of protease inhibitor and heparin increased the efficiency of resuscitation management.", "contents": "The use of protease inhibitor (trasylol) and heparin in cardiorespiratory resuscitation. I. Studies of the blood clotting system. The purpose of the study was the assessment of the effect of protease inhibitor (trasylol) and heparin on the blood clotting system during cardiorespiratory resuscitation. The studies were carried out in two experimental groups of rabbits. In group I oxygen was given, cardiac massage was applied, alkalinizing agents and drugs improving the action of the heart and the peripheral circulation were administered. In group II trasylol and heparin were given additionally. The studies proved that cardiorespiratory arrest causes development of the intravascular clotting syndrome. Resuscitation management in group I failed to correct the disturbances in the blood clotting system. On the other hand, in group II they were partly corrected. The animals in this group survived the experiment. The administration of protease inhibitor and heparin increased the efficiency of resuscitation management."}
{"id": "PMID:1080972", "title": "[Lymphocytes. Roles in cellular immunity and humoral immunity].", "content": "The lymphocytes are now considered as ubiquitous cells which play a role in delayed hypersensitivity, the rejection of grafts and antibody synthesis. The lymphocytes are essential in the immune process, for they may both recognise the antibody and initiate the response. They act either by themselves or after differentiation into specialised cells and, usually, in coordination with other cells with phagocytic functions. After briefly recalling the morphology, localisation, circulation, sites of production and heterogeneity of the lymphocytes, the authors discuss their function, describe lymphocytic transformation, and the role of T lymphocytes in delayed hypersensitivity, in the rejection of grafts and the direct cytotoxic effect. Antibody synthesis with B lymphocytes and the intervention of mononucleated cells with phagocytic activity, the cooperation of T lymphocytes are developed. The exploration of lymphocytic functions includes the recognition of an antigen by the lymphocyte, fixation of marked antigens, rosette formation, the demonstration of delayed hypersensitivity (inhibition of macrophage migration, cytotoxicity, skin tests), the estimation of antibodies and measurement of lymphocyte transformation. Certain factors, due to antigen-host reaction, regulate the lymphocytic immune reaction. One may experimentally intervene by immunostimulation and immunosuppression.", "contents": "[Lymphocytes. Roles in cellular immunity and humoral immunity]. The lymphocytes are now considered as ubiquitous cells which play a role in delayed hypersensitivity, the rejection of grafts and antibody synthesis. The lymphocytes are essential in the immune process, for they may both recognise the antibody and initiate the response. They act either by themselves or after differentiation into specialised cells and, usually, in coordination with other cells with phagocytic functions. After briefly recalling the morphology, localisation, circulation, sites of production and heterogeneity of the lymphocytes, the authors discuss their function, describe lymphocytic transformation, and the role of T lymphocytes in delayed hypersensitivity, in the rejection of grafts and the direct cytotoxic effect. Antibody synthesis with B lymphocytes and the intervention of mononucleated cells with phagocytic activity, the cooperation of T lymphocytes are developed. The exploration of lymphocytic functions includes the recognition of an antigen by the lymphocyte, fixation of marked antigens, rosette formation, the demonstration of delayed hypersensitivity (inhibition of macrophage migration, cytotoxicity, skin tests), the estimation of antibodies and measurement of lymphocyte transformation. Certain factors, due to antigen-host reaction, regulate the lymphocytic immune reaction. One may experimentally intervene by immunostimulation and immunosuppression."}
{"id": "PMID:1080970", "title": "Fibrinolysis activation after operation of spermatic cord.", "content": "The authors report a case of fibrinolytic haemorrhagic diathesis after operation of vas deferens carried out under epidural analgesia. Attention is called to the diagnostic difficulties in the rare complication of these operations. Treatment of fibrinolytic haemorrhagic diathesis is discussed stressing the advantages of trasylol, which is a drug of choice in emergency states associated with bleeding from the surface of the wound when difficulties arise in carrying out full blood clotting tests.", "contents": "Fibrinolysis activation after operation of spermatic cord. The authors report a case of fibrinolytic haemorrhagic diathesis after operation of vas deferens carried out under epidural analgesia. Attention is called to the diagnostic difficulties in the rare complication of these operations. Treatment of fibrinolytic haemorrhagic diathesis is discussed stressing the advantages of trasylol, which is a drug of choice in emergency states associated with bleeding from the surface of the wound when difficulties arise in carrying out full blood clotting tests."}
{"id": "PMID:1080973", "title": "Outcome analysis of surgical therapy for patients with angina pectoris.", "content": "Surgical therapy for coronary artery disease results in improved survival rates for selected groups of patients, particularly those with left ventricular dysfunction and/or severe coronary artery disease as evidenced by involvement of two or more vessels. Successful augmentation of coronary blood flow as shown by patient saphenous vein graft is associated with improved exercise tolerance when compared with patients in whom the grafts are occluded. Patients with impaired left centricular function as a result of their coronary artery disease appear to experience an improvement in this function with the augmentation of coronary blood flow by patent saphenous vein bypass grafts. In patients with occluded grafts and impaired left ventricular function, the left ventricular function is unchanged. Ptients with normal left ventricular function who  sustain occlusion of their grafts will probably experience a reduction in their systolic ejection fraction.", "contents": "Outcome analysis of surgical therapy for patients with angina pectoris. Surgical therapy for coronary artery disease results in improved survival rates for selected groups of patients, particularly those with left ventricular dysfunction and/or severe coronary artery disease as evidenced by involvement of two or more vessels. Successful augmentation of coronary blood flow as shown by patient saphenous vein graft is associated with improved exercise tolerance when compared with patients in whom the grafts are occluded. Patients with impaired left centricular function as a result of their coronary artery disease appear to experience an improvement in this function with the augmentation of coronary blood flow by patent saphenous vein bypass grafts. In patients with occluded grafts and impaired left ventricular function, the left ventricular function is unchanged. Ptients with normal left ventricular function who  sustain occlusion of their grafts will probably experience a reduction in their systolic ejection fraction."}
{"id": "PMID:1080974", "title": "Scopulariopsosis and hypersensitivity pneumonitis in an addict.", "content": "Granulomatosis caused by fungal spores of a soil saprophyte is a newly recognized pulmonary complication of intravenous drug addiction. Brown, non-budding spores were histologically identified in necrotic tissue, inside giant cells of sarcoidlike granulomata, and in the vicinity of focal angiitic lesions. The fungus was identified by culture as the dematiaceous Scopulariopsis brumptii. Cultural and histopathologic studies of lung biopsy specimens established the diagnosis. We showed precipitating antibodies to fungal antigen in the serum, prepared from the patient's isolate. Similar granulomatous pulmonary lesions were experimentally produced in mice by a single intravenous injection of spores of S. brumptii. The spores remained viable but did not show evidence of growth in the animal's tissue. Precipitating antibodies to fungal antigen and immediate wheal and late necrotizing type of skin reactions were shown in the challenged mice. The studies support the notion that pulmonary hypersensitivity to fungal spores was mediated by an Arthus'-type phenomenon.", "contents": "Scopulariopsosis and hypersensitivity pneumonitis in an addict. Granulomatosis caused by fungal spores of a soil saprophyte is a newly recognized pulmonary complication of intravenous drug addiction. Brown, non-budding spores were histologically identified in necrotic tissue, inside giant cells of sarcoidlike granulomata, and in the vicinity of focal angiitic lesions. The fungus was identified by culture as the dematiaceous Scopulariopsis brumptii. Cultural and histopathologic studies of lung biopsy specimens established the diagnosis. We showed precipitating antibodies to fungal antigen in the serum, prepared from the patient's isolate. Similar granulomatous pulmonary lesions were experimentally produced in mice by a single intravenous injection of spores of S. brumptii. The spores remained viable but did not show evidence of growth in the animal's tissue. Precipitating antibodies to fungal antigen and immediate wheal and late necrotizing type of skin reactions were shown in the challenged mice. The studies support the notion that pulmonary hypersensitivity to fungal spores was mediated by an Arthus'-type phenomenon."}
{"id": "PMID:1080976", "title": "Profiles of antinuclear antibodies in systemic rheumatic diseases.", "content": "The prevalence of different antinuclear antibodies was examined in systemic lupus erythematosus, rheumatoid arthritis, Sj\u00f6gren's syndrome, progressive systemic sclerosis, dermatomyositis, discoid lupus erythematosus, and mixed connective tissue disease. Antibody to Sm antigen (a nuclear acidic protein) was found almost exclusively in serums of patients with systemic lupus erythematosus, suggesting that this antibody might be a \"marker\" antibody for this disease. However, antibodies to native DNA and to nuclear ribonucleoprotein were found in a variety of systemic rheumatic diseases. In systemic lupus erythematosus, antibody to native DNA was present in high titer but in other rheumatic diseases in low titer. Antibody to nuclear ribonucleoprotein occurred in certain other systemic rheumatic diseases besides the mixed connective tissue disease syndrome. These studies showed that there were distinct profiles of antinuclear antibodies in certain systemic rheumatic diseases, characterized by the presence or absence of certain antibodies and by differences in their mean titers.", "contents": "Profiles of antinuclear antibodies in systemic rheumatic diseases. The prevalence of different antinuclear antibodies was examined in systemic lupus erythematosus, rheumatoid arthritis, Sj\u00f6gren's syndrome, progressive systemic sclerosis, dermatomyositis, discoid lupus erythematosus, and mixed connective tissue disease. Antibody to Sm antigen (a nuclear acidic protein) was found almost exclusively in serums of patients with systemic lupus erythematosus, suggesting that this antibody might be a \"marker\" antibody for this disease. However, antibodies to native DNA and to nuclear ribonucleoprotein were found in a variety of systemic rheumatic diseases. In systemic lupus erythematosus, antibody to native DNA was present in high titer but in other rheumatic diseases in low titer. Antibody to nuclear ribonucleoprotein occurred in certain other systemic rheumatic diseases besides the mixed connective tissue disease syndrome. These studies showed that there were distinct profiles of antinuclear antibodies in certain systemic rheumatic diseases, characterized by the presence or absence of certain antibodies and by differences in their mean titers."}
{"id": "PMID:1080977", "title": "[Variability of cultures and genetic variability].", "content": "Cultural selection is defined as a modification in gene frequency distributions within a population under the effects of socio-cultural factors. The conditions are examined which determine the efficiency of such a selection mechanism as a driving force for the evolution of the genetic pool of a social group in a state of relative isolation. The possible effect of the cultural variation on the genetic variation of the human species is discussed.", "contents": "[Variability of cultures and genetic variability]. Cultural selection is defined as a modification in gene frequency distributions within a population under the effects of socio-cultural factors. The conditions are examined which determine the efficiency of such a selection mechanism as a driving force for the evolution of the genetic pool of a social group in a state of relative isolation. The possible effect of the cultural variation on the genetic variation of the human species is discussed."}
{"id": "PMID:1080978", "title": "[Comparison of the karyotype of the orangutan (Pongo pygmaeus) to those of man, chimpazee, and gorilla].", "content": "Various banding thechniques were employed to compare the karyotype of the Orang-utan (Pongo pygmaeus) with those of Man, the Chimpanzee, and the Gorilla. The following was concluded: 1) Almost all the bands are common, their analogy being close to 99%. The four species derive, therefore, from a common ancestor. The rearrangements which occurred during evolution were generally balanced; 2) The analysis of rearrangements shows that the Orang-utan, then the Gorilla, then the Chimpanzee, and lastly Man were derived in that order from the common stemline.", "contents": "[Comparison of the karyotype of the orangutan (Pongo pygmaeus) to those of man, chimpazee, and gorilla]. Various banding thechniques were employed to compare the karyotype of the Orang-utan (Pongo pygmaeus) with those of Man, the Chimpanzee, and the Gorilla. The following was concluded: 1) Almost all the bands are common, their analogy being close to 99%. The four species derive, therefore, from a common ancestor. The rearrangements which occurred during evolution were generally balanced; 2) The analysis of rearrangements shows that the Orang-utan, then the Gorilla, then the Chimpanzee, and lastly Man were derived in that order from the common stemline."}
{"id": "PMID:1080979", "title": "[Gene localization in the chimpanzee (Pan troglodytes). Comparison with the factor mapping of man (Homo sapiens)].", "content": "Ten independant cellular hybrids were obtained from Chimpanzee (Pan troglodytes) fibroblasts and the murine cell line C11D. The comparison of electrophoretic and cytogenetic studies showed that 9 markers with known localizations in Man could also be localized on the homologous chromosomes of the Chimpanzee: pyrophosphate hydratase (PPH), phosphoglucomutase-1 (PGM1), and peptidase-C (Pep-C) on the No. 1; malate dehydrogenase MDH(NAD) on the No. 2; lactico dehydrogenase-A (LDH-A) on the No. 11; lactico dehydrogenase-B (LDH-B) on the No. 12; thymidine kinase (TK) on the No. 17; superoxide dismutase-1 (SOD-1) on the No. 21; glucose-6-phosphate dehydrogenase (G6PD) on the X. The localization of mannose phosphate isomerase (MPI) on the No. 7 could be excluded. One discrepancy between Chimpanzee and Man was noted: the localization of superoxide dismutase-2 (SOD-2) on the 6 is excluded.", "contents": "[Gene localization in the chimpanzee (Pan troglodytes). Comparison with the factor mapping of man (Homo sapiens)]. Ten independant cellular hybrids were obtained from Chimpanzee (Pan troglodytes) fibroblasts and the murine cell line C11D. The comparison of electrophoretic and cytogenetic studies showed that 9 markers with known localizations in Man could also be localized on the homologous chromosomes of the Chimpanzee: pyrophosphate hydratase (PPH), phosphoglucomutase-1 (PGM1), and peptidase-C (Pep-C) on the No. 1; malate dehydrogenase MDH(NAD) on the No. 2; lactico dehydrogenase-A (LDH-A) on the No. 11; lactico dehydrogenase-B (LDH-B) on the No. 12; thymidine kinase (TK) on the No. 17; superoxide dismutase-1 (SOD-1) on the No. 21; glucose-6-phosphate dehydrogenase (G6PD) on the X. The localization of mannose phosphate isomerase (MPI) on the No. 7 could be excluded. One discrepancy between Chimpanzee and Man was noted: the localization of superoxide dismutase-2 (SOD-2) on the 6 is excluded."}
{"id": "PMID:1080980", "title": "[2 cases of trisomy 11q(q23.2-- qter) with the same abnormality of external genitalia].", "content": "Two unrelated individuals are reported with the same clinical picture and trisomic for the same segment of chromosome 11 (q23 leads to qter). That both were seen because of micropenis raises the possibility that genes interfering with the reproductive function are located on chromosome 11 and that the q23 band might be a preferential breakage point of chromosome 11. Certain earlier reports support such a possibility.", "contents": "[2 cases of trisomy 11q(q23.2-- qter) with the same abnormality of external genitalia]. Two unrelated individuals are reported with the same clinical picture and trisomic for the same segment of chromosome 11 (q23 leads to qter). That both were seen because of micropenis raises the possibility that genes interfering with the reproductive function are located on chromosome 11 and that the q23 band might be a preferential breakage point of chromosome 11. Certain earlier reports support such a possibility."}
{"id": "PMID:1080981", "title": "[2 cases of trisomy 11q(q231--qter) by translocation t(11;22) (q231;q111) in 2 different families].", "content": "Two cases of partial trisomy 11q are reported, both apparently due to segregation in both patients' families of the same translocation between a n degrees 11 and a n degrees 22. R-, T-, and G- banding showed the breakage points to be 11q231 and 22q111. The clinical syndrome results from trisomy for the q231 leads to qter segment of chromosome 11.", "contents": "[2 cases of trisomy 11q(q231--qter) by translocation t(11;22) (q231;q111) in 2 different families]. Two cases of partial trisomy 11q are reported, both apparently due to segregation in both patients' families of the same translocation between a n degrees 11 and a n degrees 22. R-, T-, and G- banding showed the breakage points to be 11q231 and 22q111. The clinical syndrome results from trisomy for the q231 leads to qter segment of chromosome 11."}
{"id": "PMID:1080982", "title": "[Trisomy 11q. Individualization of a new syndrome].", "content": "The comparison of nine examples of trisomy for the distal segment of 11q permits definition of a clinical syndrome which includes the following: axial hypotonia with hypertonia of the limbs; an old-looking wrinkled face; a large beaked nose; microretrognathia with malformation of the palate; low-set ears; a prominent anterior helix; a prominent anthelix; a short neck; a narrow chest with nipples set widely apart; micropenis in the boys; congenital heart disease; renal agenesis or malformations of the urinary tract; agenesis of the thoracic girdle; dysplasia of the acetabulum; clubfeet.", "contents": "[Trisomy 11q. Individualization of a new syndrome]. The comparison of nine examples of trisomy for the distal segment of 11q permits definition of a clinical syndrome which includes the following: axial hypotonia with hypertonia of the limbs; an old-looking wrinkled face; a large beaked nose; microretrognathia with malformation of the palate; low-set ears; a prominent anterior helix; a prominent anthelix; a short neck; a narrow chest with nipples set widely apart; micropenis in the boys; congenital heart disease; renal agenesis or malformations of the urinary tract; agenesis of the thoracic girdle; dysplasia of the acetabulum; clubfeet."}
{"id": "PMID:1080983", "title": "Human acrocentric ring chromosomes and satellite association.", "content": "In four cases of human acrocentric ring chromosomes r(21)(p13q22); r(21)(p11q22); r(13)(p11q?); and R(21)(P11Q22), association of the annular with normal D and G chromosomes was found. This finding indicates that: 1) the \"satellite\" association is not terminal 2) the heterochromatic region (band p11) adjacent to the acrocentric short arm constriction contains nucleolar organizers (NOs), although bands p12 and 13 could not be excluded; and 3) band p11 probably has two different functional sites, a distal NO+ and a proximal NO-. Related studies on three C/G translocation carriers suggest a non-specific interphase interchromosomal relationship.", "contents": "Human acrocentric ring chromosomes and satellite association. In four cases of human acrocentric ring chromosomes r(21)(p13q22); r(21)(p11q22); r(13)(p11q?); and R(21)(P11Q22), association of the annular with normal D and G chromosomes was found. This finding indicates that: 1) the \"satellite\" association is not terminal 2) the heterochromatic region (band p11) adjacent to the acrocentric short arm constriction contains nucleolar organizers (NOs), although bands p12 and 13 could not be excluded; and 3) band p11 probably has two different functional sites, a distal NO+ and a proximal NO-. Related studies on three C/G translocation carriers suggest a non-specific interphase interchromosomal relationship."}
{"id": "PMID:1080984", "title": "[Trial of detection of female carriers for the ornithine-carbamyltransferase deficiency by the urine assay of crotic acid. Apropos of a family study].", "content": "Studies on the detection of female carriers of the gene responsible for ornithine carbamoyl transferase deficiency are reported. The method used was that described by Goldstein and is based on provoked acidemia after an oral protein overload. Transmission of the disease as a sex linked dominant was confirmed. The heterogeneity of the metabolic disorder is discussed.", "contents": "[Trial of detection of female carriers for the ornithine-carbamyltransferase deficiency by the urine assay of crotic acid. Apropos of a family study]. Studies on the detection of female carriers of the gene responsible for ornithine carbamoyl transferase deficiency are reported. The method used was that described by Goldstein and is based on provoked acidemia after an oral protein overload. Transmission of the disease as a sex linked dominant was confirmed. The heterogeneity of the metabolic disorder is discussed."}
{"id": "PMID:1080985", "title": "Milk casein polymorphism in the Kikuyu population.", "content": "Urea-starch-gel electrophoresis of casein samples from women of the Kikuyu tribe (central Africa) shows that this Negroid population had the same alpha- and beta-casein polymorphic pattern as Caucasoids, though their gene frequencies for the beta-locus were inverted. A new beta-casein variant (named beta-E) was identified.", "contents": "Milk casein polymorphism in the Kikuyu population. Urea-starch-gel electrophoresis of casein samples from women of the Kikuyu tribe (central Africa) shows that this Negroid population had the same alpha- and beta-casein polymorphic pattern as Caucasoids, though their gene frequencies for the beta-locus were inverted. A new beta-casein variant (named beta-E) was identified."}
{"id": "PMID:1080986", "title": "Report of a case of 46,xx/45,xx,-20 mosaicism.", "content": "A 46,XX/45,XX,-20 mosaicism was observed in a phenotypically normal female who had three grossly malformed children (two stillbirths and a perinatal death).", "contents": "Report of a case of 46,xx/45,xx,-20 mosaicism. A 46,XX/45,XX,-20 mosaicism was observed in a phenotypically normal female who had three grossly malformed children (two stillbirths and a perinatal death)."}
{"id": "PMID:1080992", "title": "Study of two acetylcholine analogs with fixed conformations.", "content": "Two acetylcholine analogs, compound M (4,4-dimethyl-2-morpholonium) and compound P (1,1-dimethyl-3-acetoxypiperidinium) which contain the N-C-C-O group of acetylcholine fixed in the gauche and trans conformations, respectively, were tested for activity on the terminal ileum of the rat, the rectus abdominis muscle of the frog, and the superior cervical ganglion of the cat. It was found that compound M stimulates the ganglion but has no affinity for receptors in either the ileum or the rectus muscle, while compound P stimulates cholinergic receptors in the ileum and in the fast fibers of the rectus muscle but acts neither on the slow fibers of the rectus muscle nor on the ganglion.", "contents": "Study of two acetylcholine analogs with fixed conformations. Two acetylcholine analogs, compound M (4,4-dimethyl-2-morpholonium) and compound P (1,1-dimethyl-3-acetoxypiperidinium) which contain the N-C-C-O group of acetylcholine fixed in the gauche and trans conformations, respectively, were tested for activity on the terminal ileum of the rat, the rectus abdominis muscle of the frog, and the superior cervical ganglion of the cat. It was found that compound M stimulates the ganglion but has no affinity for receptors in either the ileum or the rectus muscle, while compound P stimulates cholinergic receptors in the ileum and in the fast fibers of the rectus muscle but acts neither on the slow fibers of the rectus muscle nor on the ganglion."}
{"id": "PMID:1080994", "title": "Histological observations of inhibitory effect of testosterone on lymphoid regeneration in the thymus-independent areas of B mice.", "content": "High doses of testosterone were subcutaneously given to B mice, neonatally thymectomized, lethally irradiated and reconstituted with syngeneic bone marrow cells. Histological observations of the peripheral lymphoid tissues revealed that testosterone inhibited the regeneration of the thymus-independent areas which were considered to be composed mainly of B lymphocytes. This result suggests that testosterone would inhibit the differentiation of B lymphocytes.", "contents": "Histological observations of inhibitory effect of testosterone on lymphoid regeneration in the thymus-independent areas of B mice. High doses of testosterone were subcutaneously given to B mice, neonatally thymectomized, lethally irradiated and reconstituted with syngeneic bone marrow cells. Histological observations of the peripheral lymphoid tissues revealed that testosterone inhibited the regeneration of the thymus-independent areas which were considered to be composed mainly of B lymphocytes. This result suggests that testosterone would inhibit the differentiation of B lymphocytes."}
{"id": "PMID:1080993", "title": "[Bone dysplasia with dwarfism and diffuse skeletal alterations].", "content": "Six cases of a new hereditary chondrodyplasia are reported. The features are severe dwarfism, generalized hypotonia, frequent and considerable desaxations of fingers and toes. Slight facial dysmorphism with evolutive scoliosis is often associated. Osteopetrosis is diffuse and is associated with important metaphyseal widening as well as epiphyseal irregularities and often carpal and tarsal supernumerary bones. No metabolic or chromosomal abnormality was found. The relations of the disease with related types described in Larsen's syndrome are considered.", "contents": "[Bone dysplasia with dwarfism and diffuse skeletal alterations]. Six cases of a new hereditary chondrodyplasia are reported. The features are severe dwarfism, generalized hypotonia, frequent and considerable desaxations of fingers and toes. Slight facial dysmorphism with evolutive scoliosis is often associated. Osteopetrosis is diffuse and is associated with important metaphyseal widening as well as epiphyseal irregularities and often carpal and tarsal supernumerary bones. No metabolic or chromosomal abnormality was found. The relations of the disease with related types described in Larsen's syndrome are considered."}
{"id": "PMID:1080996", "title": "Ocular manifestations of hypercupremia associated with multiple myeloma.", "content": "Extensive copper infiltration of Descemet membrane of the central cornea and of the anterior and posterior surfaces of the lens is associated with marked hypercupremia related to an abnormal immunoglobulin. Observation of this spectrum of ophthalmic changes in an apparently well middle-aged woman heralded the diagnosis of a unique variety of multiple myeloma.", "contents": "Ocular manifestations of hypercupremia associated with multiple myeloma. Extensive copper infiltration of Descemet membrane of the central cornea and of the anterior and posterior surfaces of the lens is associated with marked hypercupremia related to an abnormal immunoglobulin. Observation of this spectrum of ophthalmic changes in an apparently well middle-aged woman heralded the diagnosis of a unique variety of multiple myeloma."}
{"id": "PMID:1080997", "title": "Haemobilia from pancreatic cystadenoma.", "content": "Hamobilia from a pancreatic source is a rare cause of gastrointestinal haemorrhage. Most of the reported cases have arisen from haemorrhage into a pancreatic pseudocyst, which is frequently fatal. This report describes a patient with gastrointestinal haemorrhage arising from a pancreatic cystadenoma.", "contents": "Haemobilia from pancreatic cystadenoma. Hamobilia from a pancreatic source is a rare cause of gastrointestinal haemorrhage. Most of the reported cases have arisen from haemorrhage into a pancreatic pseudocyst, which is frequently fatal. This report describes a patient with gastrointestinal haemorrhage arising from a pancreatic cystadenoma."}
{"id": "PMID:1080999", "title": "Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides a role for trisulphides.", "content": "1. The aminolaevulinate synthetase activator of fresh extracts of semi-anaerobically grown Rhodopseudomonas spheroids was resolved into two fractions by ion-exchange chromatography. One fraction was identified as cystine trisulphide (CySSSCy). Analysis of the other fraction indicated the presence of about equal amounts of glutathione trisulphide (GSSSG) and the mixed trisulphide of glutathione and cystine (GSSSCy). 2. Four further fractions with activator activity were observed on ion-exchange chromatography of extracts prepared by methods similar to those described earlier [Neuberger et al. (1973)Biochem. J. 136,491-499]. These activators were generated by the extraction procedure. Two of them have been identified as trisulphanedisulphonate (S5O62-) and additional cystine trisulphide. 3. For the series of polysulphanedisulphonates (-O3S-Sn-SO3-, n greater than or equal to 1), activator activity at muM concentrations was exhibited only by compounds with n greater than 3. This, together with the results described above, indicates that for a compound R-Sn-R' (where R and R' are organic or inorganic groups) the only structural requirement for activity is n greater than or equal to 3. 4. Oxygenation of a semipanaerobic culture of R. spheroids for 1.5h before harvesting the cells produced a decrease of more than 90% in the cellular content of cystine trisulphide and glutathione trisulphides. 5. Chromatography on DEAE-Sephadex confirmed the presence of multiple forms of aminolaevulinate synthetase in extracts of R. spheroides [Tuboi et al. (1970) Arch.Biochem. Biophys. 138,147-154]. Oxygenation of a semi-anaerobic culture resulted in the disappearance of high-activity enzyme (a-form) and the accumulation of low-activity enzyme (b-form) in the cell. Spontaneous activation [Marriott et al. (1969) Biochem. J. 111,385-394] And activation by cystine trisulphide both resulted in the almost complete conversion of the b-form into the a-form.", "contents": "Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides a role for trisulphides. 1. The aminolaevulinate synthetase activator of fresh extracts of semi-anaerobically grown Rhodopseudomonas spheroids was resolved into two fractions by ion-exchange chromatography. One fraction was identified as cystine trisulphide (CySSSCy). Analysis of the other fraction indicated the presence of about equal amounts of glutathione trisulphide (GSSSG) and the mixed trisulphide of glutathione and cystine (GSSSCy). 2. Four further fractions with activator activity were observed on ion-exchange chromatography of extracts prepared by methods similar to those described earlier [Neuberger et al. (1973)Biochem. J. 136,491-499]. These activators were generated by the extraction procedure. Two of them have been identified as trisulphanedisulphonate (S5O62-) and additional cystine trisulphide. 3. For the series of polysulphanedisulphonates (-O3S-Sn-SO3-, n greater than or equal to 1), activator activity at muM concentrations was exhibited only by compounds with n greater than 3. This, together with the results described above, indicates that for a compound R-Sn-R' (where R and R' are organic or inorganic groups) the only structural requirement for activity is n greater than or equal to 3. 4. Oxygenation of a semipanaerobic culture of R. spheroids for 1.5h before harvesting the cells produced a decrease of more than 90% in the cellular content of cystine trisulphide and glutathione trisulphides. 5. Chromatography on DEAE-Sephadex confirmed the presence of multiple forms of aminolaevulinate synthetase in extracts of R. spheroides [Tuboi et al. (1970) Arch.Biochem. Biophys. 138,147-154]. Oxygenation of a semi-anaerobic culture resulted in the disappearance of high-activity enzyme (a-form) and the accumulation of low-activity enzyme (b-form) in the cell. Spontaneous activation [Marriott et al. (1969) Biochem. J. 111,385-394] And activation by cystine trisulphide both resulted in the almost complete conversion of the b-form into the a-form."}
{"id": "PMID:1081000", "title": "Membrane receptor sites for the identification of lymphoreticular cells in benign and malignant conditions.", "content": "The cells of the lymphoreticular system are heterogeneous both morphologically and functionally. The bone marrow derived (B) lymphocyte can be identified by the presence of easily detectable surface immunoglobulin and a receptor for antigen-antibody-complement (EAC) complexes. Monocytes and histiocytes also bear a receptor for EAC and in addition possess a receptor for cytophilic antibody detected with red cell--IgG complexes (IgGEA). In man, thymus derived (T) lymphocytes form non-immune rosettes with sheep red blood cells (E). We have examined a number of malignant lymphoreticular populations for the presence of the EAC, EA, and E receptors on suspensions of cells and have adapted the technique to demonstrate the EAC and EA receptors on  frozen tissue sections. Rosetted malignant cells can also be cytologically examined on Millipore filters. The malignant cells both in section and suspension from the spleens and lymph nodes of 6 patients with nodular lymphoma bound EAC but not IgGEA or E; by these criteria these malignant cells are of B lymphocytic origin. The malignant cells from the spleens of 2 patients with leukaemic reticuloendotheliosis and 1 patient with malignant histiocytosis could be classified as being of histiocytic origin by the selective binding of IgGEA. In 3 cases of diffuse lymphocytic lymphoma the malignant cells bound only E and are therefore of T lymphocytic origin. The application of these techniques to the classification of malignant lymphoma may lead to important theoretical and therapeutic advances.", "contents": "Membrane receptor sites for the identification of lymphoreticular cells in benign and malignant conditions. The cells of the lymphoreticular system are heterogeneous both morphologically and functionally. The bone marrow derived (B) lymphocyte can be identified by the presence of easily detectable surface immunoglobulin and a receptor for antigen-antibody-complement (EAC) complexes. Monocytes and histiocytes also bear a receptor for EAC and in addition possess a receptor for cytophilic antibody detected with red cell--IgG complexes (IgGEA). In man, thymus derived (T) lymphocytes form non-immune rosettes with sheep red blood cells (E). We have examined a number of malignant lymphoreticular populations for the presence of the EAC, EA, and E receptors on suspensions of cells and have adapted the technique to demonstrate the EAC and EA receptors on  frozen tissue sections. Rosetted malignant cells can also be cytologically examined on Millipore filters. The malignant cells both in section and suspension from the spleens and lymph nodes of 6 patients with nodular lymphoma bound EAC but not IgGEA or E; by these criteria these malignant cells are of B lymphocytic origin. The malignant cells from the spleens of 2 patients with leukaemic reticuloendotheliosis and 1 patient with malignant histiocytosis could be classified as being of histiocytic origin by the selective binding of IgGEA. In 3 cases of diffuse lymphocytic lymphoma the malignant cells bound only E and are therefore of T lymphocytic origin. The application of these techniques to the classification of malignant lymphoma may lead to important theoretical and therapeutic advances."}
{"id": "PMID:1081001", "title": "Evaluation of T and B lymphocyte membrane markers in human non-Hodgkin malignant lymphomata.", "content": "Lymphoma cells from 25 patients were studied for the presence of B lymphocytes (membrane bound Ig and Fc receptor) and T lymphocytes (rosette formation with sheep erythrocytes) membrane markers. All cases of well differentiated lymphocytic lymphoma and of acute lymphosarcoma cell leukaemia and most cases of poorly differentiated lymphocytic lymphoma behaved as B cell monoclonal malignancies. However, the malignant cells of some patients were not definitely classified according to their B or T cell origin or lacked these membrane markers. The latter situation was encountered in 4 reticulum cell sarcomata. Polyclonal Ig were found on the surface of B cells in a case of hyperbasophilic undifferentiated lymphoma. The need for using several membrane markers to study the abnormal lymphoma cells is outlined. Such studies improve our understanding of these malignancies and may lead in the future to a satisfactory classification of non-Hodgkin lymphomata.", "contents": "Evaluation of T and B lymphocyte membrane markers in human non-Hodgkin malignant lymphomata. Lymphoma cells from 25 patients were studied for the presence of B lymphocytes (membrane bound Ig and Fc receptor) and T lymphocytes (rosette formation with sheep erythrocytes) membrane markers. All cases of well differentiated lymphocytic lymphoma and of acute lymphosarcoma cell leukaemia and most cases of poorly differentiated lymphocytic lymphoma behaved as B cell monoclonal malignancies. However, the malignant cells of some patients were not definitely classified according to their B or T cell origin or lacked these membrane markers. The latter situation was encountered in 4 reticulum cell sarcomata. Polyclonal Ig were found on the surface of B cells in a case of hyperbasophilic undifferentiated lymphoma. The need for using several membrane markers to study the abnormal lymphoma cells is outlined. Such studies improve our understanding of these malignancies and may lead in the future to a satisfactory classification of non-Hodgkin lymphomata."}
{"id": "PMID:1081002", "title": "Action of aldosterone on frog skin in the presence and absence of in vitro molting effects.", "content": "The molting which occurs in frog skin following exposure to high concentrations of aldosterone interferes with the interpretation of physiological measurements. Exposure of skins from frogs maintained in standard smooth tanks to 5 - 10(-7) M aldosterone caused within a few hours erratic responses in short-circuit current Io and conductance K followed by sustained stimulation of Io and K; 10(-8) M aldosterone caused only stimulation of Io and K. Storage of frogs in \"rojgh tanks\" eliminated in vitro molting on exposure to 5 - 10(-7) M aldosterone. IO and K were then superimposable for 3 h, after which Io increased far more rapidly than K. These results are consistent with an early effect on permeability of the active pathway and later effects on metabolism, either a direct effect on the pump or enhanced interaction between transport and metabolism.", "contents": "Action of aldosterone on frog skin in the presence and absence of in vitro molting effects. The molting which occurs in frog skin following exposure to high concentrations of aldosterone interferes with the interpretation of physiological measurements. Exposure of skins from frogs maintained in standard smooth tanks to 5 - 10(-7) M aldosterone caused within a few hours erratic responses in short-circuit current Io and conductance K followed by sustained stimulation of Io and K; 10(-8) M aldosterone caused only stimulation of Io and K. Storage of frogs in \"rojgh tanks\" eliminated in vitro molting on exposure to 5 - 10(-7) M aldosterone. IO and K were then superimposable for 3 h, after which Io increased far more rapidly than K. These results are consistent with an early effect on permeability of the active pathway and later effects on metabolism, either a direct effect on the pump or enhanced interaction between transport and metabolism."}
{"id": "PMID:1081003", "title": "A rapid method for purification of human granulocyte cationic neutral proteases: purification and characterization of human granulocyte chymotrypsin-like enzyme.", "content": "A chymotrypsin-like enzyme (EC 3.4.21.-) was purified from granules of human neutrophiles (polymorphonuclear leucocytes). The isolation procedure included differential salt extractions of the granules followed by affinity chromatography on 4-phenylbutylamine-Affi-Gel. This rapid purification method resulted in obtaining pure enzyme in relatively high yield in short time. The purified granulocyte chymotrypsin-like enzyme has a minimum Mr of 22 378, calculated from its amino acid composition. The Mr value obtained by sodium dodecyl sulphate gel electrophoresis was 20 000-23 000. The enzyme did not react with antibodies which are monospecific to granulocyte elastase. The granulocyte chymotrypsin-like enzyme was inactivated by Dip-F and by the chloromethyl ketone derivatives Z-PheCH2Cl and Z-(Gly)2-PheCH2Cl but not by Tos-PheCH2Cl. It therefore appears that the enzyme has serine and histidine side chains in its active site, like pancreatic chymotrypsin. The granulocyte enzyme substrate specificity is similar to that of pac-Tyr-Nan and Ac-Phe-1-ONap. It also has an intrinsic weak hydrolytic activity towards some classical elastase substrates such as Boc-Ala-ONp and Ac-DL-Ala-1-ONap. The granulocyte enzyme is inhibited by human serum and by human alpha1-antitrypsin. Its affinity for alpha1-antitrypsin is weaker than that of granulocyte elastase for the same inhibitor. The enzyme is stable at neutral pH at 37 degrees C, but unstable at pH 3.5 and at elevated temperature.", "contents": "A rapid method for purification of human granulocyte cationic neutral proteases: purification and characterization of human granulocyte chymotrypsin-like enzyme. A chymotrypsin-like enzyme (EC 3.4.21.-) was purified from granules of human neutrophiles (polymorphonuclear leucocytes). The isolation procedure included differential salt extractions of the granules followed by affinity chromatography on 4-phenylbutylamine-Affi-Gel. This rapid purification method resulted in obtaining pure enzyme in relatively high yield in short time. The purified granulocyte chymotrypsin-like enzyme has a minimum Mr of 22 378, calculated from its amino acid composition. The Mr value obtained by sodium dodecyl sulphate gel electrophoresis was 20 000-23 000. The enzyme did not react with antibodies which are monospecific to granulocyte elastase. The granulocyte chymotrypsin-like enzyme was inactivated by Dip-F and by the chloromethyl ketone derivatives Z-PheCH2Cl and Z-(Gly)2-PheCH2Cl but not by Tos-PheCH2Cl. It therefore appears that the enzyme has serine and histidine side chains in its active site, like pancreatic chymotrypsin. The granulocyte enzyme substrate specificity is similar to that of pac-Tyr-Nan and Ac-Phe-1-ONap. It also has an intrinsic weak hydrolytic activity towards some classical elastase substrates such as Boc-Ala-ONp and Ac-DL-Ala-1-ONap. The granulocyte enzyme is inhibited by human serum and by human alpha1-antitrypsin. Its affinity for alpha1-antitrypsin is weaker than that of granulocyte elastase for the same inhibitor. The enzyme is stable at neutral pH at 37 degrees C, but unstable at pH 3.5 and at elevated temperature."}
{"id": "PMID:1081004", "title": "Structure and active transport in the plasma membrane of the tubules of frog kidney.", "content": "The active transport of organic anions through the plasma membrane of the proximal tubules of frog kidney was studied. For this purpose a marker anion, fluorescein, was used, its flow into the tubules registered by the increase of fluorescense. The kinetics of transport was measured as function of time, concentration of substrate, concentration of a competing acid (p-aminohippuric acid) and temperature. The process is inhibited by strophantin, a specific poison for (Na++K+)-dependent ATPase. These data show that fluorescein transport is effected with the participation of a charged carrier, probably by the downfield mechanism postulated by Mitchell. To confirm this mechanism, a passive flow of K+ was created inwards across the membrane of the proximal tubules by means of valinomycin. It led to the discharge of the membrane and to the inhibition of fluorescein transport. Anions are transported downfield across the membrane, probably in a state of complexes with two Na+ ions. A magnetic field of 10000-28000 oersted inhibits the fluorescein transport strongly. This can be regarded as a proof of the liquid-crystalline structure of biological membranes and demonstrates the importance of this structure for active transport.", "contents": "Structure and active transport in the plasma membrane of the tubules of frog kidney. The active transport of organic anions through the plasma membrane of the proximal tubules of frog kidney was studied. For this purpose a marker anion, fluorescein, was used, its flow into the tubules registered by the increase of fluorescense. The kinetics of transport was measured as function of time, concentration of substrate, concentration of a competing acid (p-aminohippuric acid) and temperature. The process is inhibited by strophantin, a specific poison for (Na++K+)-dependent ATPase. These data show that fluorescein transport is effected with the participation of a charged carrier, probably by the downfield mechanism postulated by Mitchell. To confirm this mechanism, a passive flow of K+ was created inwards across the membrane of the proximal tubules by means of valinomycin. It led to the discharge of the membrane and to the inhibition of fluorescein transport. Anions are transported downfield across the membrane, probably in a state of complexes with two Na+ ions. A magnetic field of 10000-28000 oersted inhibits the fluorescein transport strongly. This can be regarded as a proof of the liquid-crystalline structure of biological membranes and demonstrates the importance of this structure for active transport."}
{"id": "PMID:1081005", "title": "Alpha1-antitrypsin concentration in amniotic fluid.", "content": "Concentrations of alpha1-antitrypsin were measured in 80 specimens of amniotic fluid collected at various stages of pregnancy. Levels remained relatively unchanged throughout gestation, with a median concentration of 10.0 mg/100 ml at 12-24 weeks, 18.7 mg/100 ml at 34-37 weeks, and 16.2 mg/100 ml, after the 37th week. In three instances where the infant developed RDS, the median concentration was 12.4 mg/100 ml.", "contents": "Alpha1-antitrypsin concentration in amniotic fluid. Concentrations of alpha1-antitrypsin were measured in 80 specimens of amniotic fluid collected at various stages of pregnancy. Levels remained relatively unchanged throughout gestation, with a median concentration of 10.0 mg/100 ml at 12-24 weeks, 18.7 mg/100 ml at 34-37 weeks, and 16.2 mg/100 ml, after the 37th week. In three instances where the infant developed RDS, the median concentration was 12.4 mg/100 ml."}
{"id": "PMID:1081006", "title": "Radiology in the diagnosis of colloid cysts of the third ventricle.", "content": "A series of 38 cases of colloid cyst of the 3rd ventricle is described in most of whom the diagnosis, important for curative surgery, was made radiologically. The most useful investigative technique was pneumography, by which the tumour was outlined in almost all cases. The lateral \"hanging head\" projection generally demonstrated the lesion most satisfactorily. Care in manipulating air introduced at ventriculography through the foramina of Monro into the 3rd ventricle was frequently rewarded by clear definition of the tumour. In more than one-third of the cases, the lateral plain skull radiograph showed truncation of the dorsum sellae indicative of chronic obstructive hydrocephalus, and, in the large majority of these, pneumography confirmed that the 3rd ventricle, rather than the interventricular foramen, was the site of obstruction to the drainage of cerebrospinal fluid. Carotid angiography, showing hydrocephalus, and in the same cases elevation and lateral displacement of the anterior portion of the internal cerebral veins yielded strong supportive evidence. Vertebral angiography was less specific, and isotope scanning was quite unhelpful.", "contents": "Radiology in the diagnosis of colloid cysts of the third ventricle. A series of 38 cases of colloid cyst of the 3rd ventricle is described in most of whom the diagnosis, important for curative surgery, was made radiologically. The most useful investigative technique was pneumography, by which the tumour was outlined in almost all cases. The lateral \"hanging head\" projection generally demonstrated the lesion most satisfactorily. Care in manipulating air introduced at ventriculography through the foramina of Monro into the 3rd ventricle was frequently rewarded by clear definition of the tumour. In more than one-third of the cases, the lateral plain skull radiograph showed truncation of the dorsum sellae indicative of chronic obstructive hydrocephalus, and, in the large majority of these, pneumography confirmed that the 3rd ventricle, rather than the interventricular foramen, was the site of obstruction to the drainage of cerebrospinal fluid. Carotid angiography, showing hydrocephalus, and in the same cases elevation and lateral displacement of the anterior portion of the internal cerebral veins yielded strong supportive evidence. Vertebral angiography was less specific, and isotope scanning was quite unhelpful."}
{"id": "PMID:1081007", "title": "Trigger factors and HL-A antigens in chronic active hepatitis.", "content": "Forty-six patients with histologically verified chronic active hepatitis (CAH) were divided into three groups according to whether the CAH was virus-induced, drug-induced, or cryptogenic. The frequency of the HL-A antigens 1 and 8 was increased in the cryptogenic group while the other groups did not differ significantly from healthy controls. Autoantibodies were often found in high titres in the drug-induced and cryptogenic groups but were infrequent in the virus-induced group.", "contents": "Trigger factors and HL-A antigens in chronic active hepatitis. Forty-six patients with histologically verified chronic active hepatitis (CAH) were divided into three groups according to whether the CAH was virus-induced, drug-induced, or cryptogenic. The frequency of the HL-A antigens 1 and 8 was increased in the cryptogenic group while the other groups did not differ significantly from healthy controls. Autoantibodies were often found in high titres in the drug-induced and cryptogenic groups but were infrequent in the virus-induced group."}
{"id": "PMID:1081008", "title": "Spatial and temporal organization of the binocular input to frog optic tectum.", "content": "Single-unit recordings in the frog's optic tectum have demonstrated the existence of a systematic spatial separation between the direct contralateral and indirect ipsilateral excitatory receptive fields. Marked differences in this spatial organization were found between paralyzed and anesthesized animals. Significant latency differences were found between sustained (class I/II) and transient (class III) contralateral fibers. Corresponding latency differences were also seen in ipsilaterally driven responses. It is suggested that there may be at least two different classes of ipsilateral fibers. The existence of binocular interaction at the level of the afferent terminal arborizations was investigated, utilizing temporally asynchronous dichoptic stimulation. No such phenomena were seen in curarized animals. These findings are discussed in terms of possible velocity and direction sensitivity mechanisms.", "contents": "Spatial and temporal organization of the binocular input to frog optic tectum. Single-unit recordings in the frog's optic tectum have demonstrated the existence of a systematic spatial separation between the direct contralateral and indirect ipsilateral excitatory receptive fields. Marked differences in this spatial organization were found between paralyzed and anesthesized animals. Significant latency differences were found between sustained (class I/II) and transient (class III) contralateral fibers. Corresponding latency differences were also seen in ipsilaterally driven responses. It is suggested that there may be at least two different classes of ipsilateral fibers. The existence of binocular interaction at the level of the afferent terminal arborizations was investigated, utilizing temporally asynchronous dichoptic stimulation. No such phenomena were seen in curarized animals. These findings are discussed in terms of possible velocity and direction sensitivity mechanisms."}
{"id": "PMID:1081017", "title": "Significance of new Q waves after aortocoronary bypass surgery. Correlation with changes in ventricular wall motion.", "content": "From June 1969 to December 1974, 142 patients underwent aortocoronary bypass surgery at the Veterans Administration Hospital, San Francisco. Seventeen patients developed new Q waves; two of four deaths in the hospital occurred among these 17 patients. Preoperative and postoperative catheterization data were available for analysis in 12 of the 15 surviving patients. Longitudinal, transverse, and hemiaxial segmental analysis of preoperative and postoperative single plane, 30 degrees right anterior oblique left ventriculograms revealed deterioration in wall motion in all 12 patients. The deterioration corresponded to the area on the electrocardiogram where the new Q waves appeared. In ten of 12 patients, ejection fraction also deteriorated. Twenty-six of 29 grafts were patent. New Q waves after aortocoronary bypass surgery correlate well with the appearance of localized abnormalities in wall motion. We found no evidence that perioperative appearance of Q waves was not due to new perioperative myocardial infarction.", "contents": "Significance of new Q waves after aortocoronary bypass surgery. Correlation with changes in ventricular wall motion. From June 1969 to December 1974, 142 patients underwent aortocoronary bypass surgery at the Veterans Administration Hospital, San Francisco. Seventeen patients developed new Q waves; two of four deaths in the hospital occurred among these 17 patients. Preoperative and postoperative catheterization data were available for analysis in 12 of the 15 surviving patients. Longitudinal, transverse, and hemiaxial segmental analysis of preoperative and postoperative single plane, 30 degrees right anterior oblique left ventriculograms revealed deterioration in wall motion in all 12 patients. The deterioration corresponded to the area on the electrocardiogram where the new Q waves appeared. In ten of 12 patients, ejection fraction also deteriorated. Twenty-six of 29 grafts were patent. New Q waves after aortocoronary bypass surgery correlate well with the appearance of localized abnormalities in wall motion. We found no evidence that perioperative appearance of Q waves was not due to new perioperative myocardial infarction."}
{"id": "PMID:1081018", "title": "Plasma and atrial propranolol after preoperative withdrawal.", "content": "Of 34 patients scheduled for aortocoronary bypass operations who were withdrawn from chronic propranolol therapy between 18 and 48 hours before anesthesia and operation, propranolol was detected in the plasma of nine and in atrial tissue of five patients. Higher plasma levels were associated with higher propranolol intake and with shorter time of withdrawal. In 15 patients withdrawn preoperatively from propranolol the heart rate and blood pressure response to 3 mug isoproterenol was not significantly different from the response of similar patients who had received no propranolol. In contrast to the response of healthy subjects, most patients with coronary artery disease responded to isoproterenol with a decrease in systolic blood pressure. Plasma propranolol levels after a 0.5 mg bolus given intravenously during operation produced a peak level of 40 ng/ml in one minute and disappeared in five minutes. These studies suggest that no beta blockade persists 18 hours after acute preoperative withdrawal of propranolol in patients taking up to 300 mg per day for control of angina. In view of the reported hazards of abrupt withdrawal of propranolol before operation and the reported usefulness of propranolol in treating tachyarrhythmias during operation, we suggest that early preoperative withdrawal of propranolol may be more hazardous than its continuation before coronary bypass operations.", "contents": "Plasma and atrial propranolol after preoperative withdrawal. Of 34 patients scheduled for aortocoronary bypass operations who were withdrawn from chronic propranolol therapy between 18 and 48 hours before anesthesia and operation, propranolol was detected in the plasma of nine and in atrial tissue of five patients. Higher plasma levels were associated with higher propranolol intake and with shorter time of withdrawal. In 15 patients withdrawn preoperatively from propranolol the heart rate and blood pressure response to 3 mug isoproterenol was not significantly different from the response of similar patients who had received no propranolol. In contrast to the response of healthy subjects, most patients with coronary artery disease responded to isoproterenol with a decrease in systolic blood pressure. Plasma propranolol levels after a 0.5 mg bolus given intravenously during operation produced a peak level of 40 ng/ml in one minute and disappeared in five minutes. These studies suggest that no beta blockade persists 18 hours after acute preoperative withdrawal of propranolol in patients taking up to 300 mg per day for control of angina. In view of the reported hazards of abrupt withdrawal of propranolol before operation and the reported usefulness of propranolol in treating tachyarrhythmias during operation, we suggest that early preoperative withdrawal of propranolol may be more hazardous than its continuation before coronary bypass operations."}
{"id": "PMID:1081019", "title": "Screening for cystic fibrosis by analysis of meconium for albumin and protease inhibitors.", "content": "A qualitative method of detecting elevated meconium protein concentration was compared with a method of determining meconium albumin concentration by electroimmunoassay since elevated meconium protein levels can indicate pancreatic insufficiency caused by cystic fibrosis. Between 5 and 10 per 1000 healthy infants passed meconium specimens that gave a false positive reaction with the Boehringer Mannheim test strip and contained a greater than expected concentration of albumin.  It was possible to exclude pancreatic insufficiency in all of these children by determining the ratio, albumin : alpha1-antitrypsin in meconium and subsequent faecal specimens, since it was found that values of this ratio in excess of 2.0 suggested pancreatic insufficiency of the type associated with cystic fibrosis. Three of 14 neonates with subsequently proven cystic fibrosis yielded meconium specimens giving negative test strip results and low albumin concentrations. In two of these patients, the ratio, albumin : alpha1-antitrypsin in the meconium was within normal limits but, within two months of birth, the albumin : alpha1-antitrypsin ratio in the faeces of both children was greater than 3.0 suggesting that pancreatic insufficiency had developed.", "contents": "Screening for cystic fibrosis by analysis of meconium for albumin and protease inhibitors. A qualitative method of detecting elevated meconium protein concentration was compared with a method of determining meconium albumin concentration by electroimmunoassay since elevated meconium protein levels can indicate pancreatic insufficiency caused by cystic fibrosis. Between 5 and 10 per 1000 healthy infants passed meconium specimens that gave a false positive reaction with the Boehringer Mannheim test strip and contained a greater than expected concentration of albumin.  It was possible to exclude pancreatic insufficiency in all of these children by determining the ratio, albumin : alpha1-antitrypsin in meconium and subsequent faecal specimens, since it was found that values of this ratio in excess of 2.0 suggested pancreatic insufficiency of the type associated with cystic fibrosis. Three of 14 neonates with subsequently proven cystic fibrosis yielded meconium specimens giving negative test strip results and low albumin concentrations. In two of these patients, the ratio, albumin : alpha1-antitrypsin in the meconium was within normal limits but, within two months of birth, the albumin : alpha1-antitrypsin ratio in the faeces of both children was greater than 3.0 suggesting that pancreatic insufficiency had developed."}
{"id": "PMID:1081020", "title": "The potentiating, mitogenic and inhibitory effects on lymphocytes in vitro, of macrophages in the lymph nodes of mice 'overloaded' with mycobacterial products.", "content": "The lymph nodes of mice overloaded with mycobacterial products, either by the injection of whole or ultrasonicated organisms, or as a consequence of severe infection with Mycobacterium ulcerans, contain phagocytic cells which cause spontaneous transformation of the lymph node cells in a low volume, high cell density culture system. This spontaneous mitosis is unaffected by trypsinization but is inhibited by specific antigen and by PHA, and eliminated by treatment with carbonyl iron. Replacement of the macrophages removed with carbonyl iron by a critical number of peritoneal cells, restores the spontaneous transformation. Normal lymph node, thymus or peritoneal lymphocytes will also undergo mitosis if small numbers of peritoneal cells are added to them. This phenomenon therefore appears not to be antigen-dependent, but is probably due to a mediator released from macrophages. The possible role of this phenomenon in the pathogenesis of mycobacterial disease and the 'overloading' of T lymphocytes in vivo is discussed, with reference to similar macrophage-dependent mechanisms reported in other systems.", "contents": "The potentiating, mitogenic and inhibitory effects on lymphocytes in vitro, of macrophages in the lymph nodes of mice 'overloaded' with mycobacterial products. The lymph nodes of mice overloaded with mycobacterial products, either by the injection of whole or ultrasonicated organisms, or as a consequence of severe infection with Mycobacterium ulcerans, contain phagocytic cells which cause spontaneous transformation of the lymph node cells in a low volume, high cell density culture system. This spontaneous mitosis is unaffected by trypsinization but is inhibited by specific antigen and by PHA, and eliminated by treatment with carbonyl iron. Replacement of the macrophages removed with carbonyl iron by a critical number of peritoneal cells, restores the spontaneous transformation. Normal lymph node, thymus or peritoneal lymphocytes will also undergo mitosis if small numbers of peritoneal cells are added to them. This phenomenon therefore appears not to be antigen-dependent, but is probably due to a mediator released from macrophages. The possible role of this phenomenon in the pathogenesis of mycobacterial disease and the 'overloading' of T lymphocytes in vivo is discussed, with reference to similar macrophage-dependent mechanisms reported in other systems."}
{"id": "PMID:1081021", "title": "Immunodeficiency diseases. I. T-lymphocyte precursors and T-lymphocyte differentiation in partial Di George syndrome.", "content": "Immunological and pathological studies in a case of partial Di George syndrome revealed an absence of parathyroids, a major hypoplasia of thymus but a relatively moderate decrease in peripheral T-lymphocyte numbers and functions. After in vitro incubation with normal thymus extracts, a normal proportion of bone marrow cells was induced to differentiate into cells with characteristics of T lymphocytes, thus establishing the presence of T-cell precursors in the patient's bone marrow.", "contents": "Immunodeficiency diseases. I. T-lymphocyte precursors and T-lymphocyte differentiation in partial Di George syndrome. Immunological and pathological studies in a case of partial Di George syndrome revealed an absence of parathyroids, a major hypoplasia of thymus but a relatively moderate decrease in peripheral T-lymphocyte numbers and functions. After in vitro incubation with normal thymus extracts, a normal proportion of bone marrow cells was induced to differentiate into cells with characteristics of T lymphocytes, thus establishing the presence of T-cell precursors in the patient's bone marrow."}
{"id": "PMID:1081022", "title": "Increased proliferation of T lymphocytes in the blood of patients with Hodgkin's disease.", "content": "We investigated the number of DNA-synthesizing T lymphocytes in the blood of patients with Hodgkin's disease, with infectious mononucleosis and in normal controls. T cells were characterized by their ability to form rosettes with unsensitized neuramidase-treated sheep red blood cells. Cells in DNA synthesis were evaluated autoradiographically after in vitro incubation with [3H]thymidine. Our results indicated a preferential proliferation of T lymphocytes in the blood of patients with Hodgkin's disease and infectious mononucleosis and suggested an increased turnover of these cells.", "contents": "Increased proliferation of T lymphocytes in the blood of patients with Hodgkin's disease. We investigated the number of DNA-synthesizing T lymphocytes in the blood of patients with Hodgkin's disease, with infectious mononucleosis and in normal controls. T cells were characterized by their ability to form rosettes with unsensitized neuramidase-treated sheep red blood cells. Cells in DNA synthesis were evaluated autoradiographically after in vitro incubation with [3H]thymidine. Our results indicated a preferential proliferation of T lymphocytes in the blood of patients with Hodgkin's disease and infectious mononucleosis and suggested an increased turnover of these cells."}
{"id": "PMID:1081023", "title": "Myasthenia gravis: studies on HL-A antigens and lymphocyte subpopulations in patients with myasthenia gravis.", "content": "Thirth-three patient with a clinical diagnosis of myasthenia gravis were tissue-typed for HL-A antigens. In agreement with earlier reports a significant increase in antigens HL-A1 and HL-A8 were found in this material. Two of the patients were treated with chronic thoracic duct drainage. Proportions of T and B lymphocytes in lymph and peripheral blood were estimated in these patients. In the lymph an initial decrease in the proportion of T cells occurred, which was accompanied by a subsequent increase in the proportion of B cells. Towards the end of the chronic drainage period this effect was reversed. A slightly different picture occurred in blood lymphocytes. Initially, there was an increase in both T and B cells, followed by a decrease in T-cells numbers in one patient, whereas in the second patient the proportion of T cells decreased from the onset of drainage while the proportion of B cells steadily increased. These studies showed that available markers for determination of T ANd B cells were useful for studies of lymphocyte subpopulations in blood and lymph. Lmyphocytes from the thoracic duct were also tested for their reactivity to various mitogens specific for either T or B cells. The B-cell mitogens which were used were dextran sulphate, lipopolysaccharide, purified protein derivative, as well as rabbit anti-human beta2-microglobulin serum. The T-cell mitogens investigated were concanavalin A and phytohaemagglutinin. No significant differences in the responsiveness of thoracic duct lymphocytes compared to normal peripheral blood lymphocytes were found.", "contents": "Myasthenia gravis: studies on HL-A antigens and lymphocyte subpopulations in patients with myasthenia gravis. Thirth-three patient with a clinical diagnosis of myasthenia gravis were tissue-typed for HL-A antigens. In agreement with earlier reports a significant increase in antigens HL-A1 and HL-A8 were found in this material. Two of the patients were treated with chronic thoracic duct drainage. Proportions of T and B lymphocytes in lymph and peripheral blood were estimated in these patients. In the lymph an initial decrease in the proportion of T cells occurred, which was accompanied by a subsequent increase in the proportion of B cells. Towards the end of the chronic drainage period this effect was reversed. A slightly different picture occurred in blood lymphocytes. Initially, there was an increase in both T and B cells, followed by a decrease in T-cells numbers in one patient, whereas in the second patient the proportion of T cells decreased from the onset of drainage while the proportion of B cells steadily increased. These studies showed that available markers for determination of T ANd B cells were useful for studies of lymphocyte subpopulations in blood and lymph. Lmyphocytes from the thoracic duct were also tested for their reactivity to various mitogens specific for either T or B cells. The B-cell mitogens which were used were dextran sulphate, lipopolysaccharide, purified protein derivative, as well as rabbit anti-human beta2-microglobulin serum. The T-cell mitogens investigated were concanavalin A and phytohaemagglutinin. No significant differences in the responsiveness of thoracic duct lymphocytes compared to normal peripheral blood lymphocytes were found."}
{"id": "PMID:1081024", "title": "Studies on the cytotoxic effect of in vivo and in vitro immunized lymphocytes on liver target cells.", "content": "Lymph node and spleen cells from mice immunized in vivo to allogeneic of syngeneic liver antigen are cytotoxic for syngeneic liver cells, but not for syngeneic fibroblasts or established liver cell cultures of allogeneic origin. The cytotoxic activity is mainly dependent on T-cell activity, but a non-T-cell-mediated cytotoxicity may also play a role. Lymphocytotoxicity is inhibited by preincubation of the lymphocytes with syngeneic liver antigen, but not with syngeneic kidney homogenate. The liver-specific lymphocytotoxicity corresponds to the in vivo function of lymphocytes in the development of experimental hepatitis. In vitro immunization of lymphocytes in a Mishell-Dutton culture system also induces liver-specific cytotoxicity. The results indicate that the natural tolerance to self antigens can be lost after invivo as well as in vitro immunization. The induction of self-reactivity of lymphocytes in these experiments may be attributed to regulatory mechanisms of the immune reaction at a cellular level.", "contents": "Studies on the cytotoxic effect of in vivo and in vitro immunized lymphocytes on liver target cells. Lymph node and spleen cells from mice immunized in vivo to allogeneic of syngeneic liver antigen are cytotoxic for syngeneic liver cells, but not for syngeneic fibroblasts or established liver cell cultures of allogeneic origin. The cytotoxic activity is mainly dependent on T-cell activity, but a non-T-cell-mediated cytotoxicity may also play a role. Lymphocytotoxicity is inhibited by preincubation of the lymphocytes with syngeneic liver antigen, but not with syngeneic kidney homogenate. The liver-specific lymphocytotoxicity corresponds to the in vivo function of lymphocytes in the development of experimental hepatitis. In vitro immunization of lymphocytes in a Mishell-Dutton culture system also induces liver-specific cytotoxicity. The results indicate that the natural tolerance to self antigens can be lost after invivo as well as in vitro immunization. The induction of self-reactivity of lymphocytes in these experiments may be attributed to regulatory mechanisms of the immune reaction at a cellular level."}
{"id": "PMID:1081030", "title": "T and B origin of human lymphocytic leukaemia.", "content": "A new immunological classification of leukaemia diseases in man was assessed by using electrophoretic mobility as a cell-marker. It was found that all acute lymphocytic leukaemia analysed in childhood were T-cell leukaemia, and the majority of chronic lymphocytic leukaemia in adults were also T-cell leukaemia. The remainder, chronic lymphocytic leukaemia in adults, were B-cell leukaemia. The above statements are only partially compatible with the classification given by the most commonly used T- and B-cell markers such as rosette formation or cell-surface immunoglobulins.", "contents": "T and B origin of human lymphocytic leukaemia. A new immunological classification of leukaemia diseases in man was assessed by using electrophoretic mobility as a cell-marker. It was found that all acute lymphocytic leukaemia analysed in childhood were T-cell leukaemia, and the majority of chronic lymphocytic leukaemia in adults were also T-cell leukaemia. The remainder, chronic lymphocytic leukaemia in adults, were B-cell leukaemia. The above statements are only partially compatible with the classification given by the most commonly used T- and B-cell markers such as rosette formation or cell-surface immunoglobulins."}
{"id": "PMID:1081031", "title": "The so-called Dandy-Walker syndrome: analysis of 12 operated cases.", "content": "Twelve patients between the years of 1964 and 1971 underwent surgical treatment for the so-called Dandy-Walker syndrome at the Johns Hopkins Hospital. Five patients belonged to an early group (1-8 weeks); four patients to an intermediary group (3-20 months); and a late group consisted of three patients (3 years, 14 years and 34 years). The value of the various diagnostic studies is discussed on the basis of an analysis of the plain skull X-rays, cerebral angiograms, and combined air studies. The importance of a careful delineation of the underlying pathological condition is stressed to enable the surgeon to choose the appropriate technique. The diagnostic studies confirmed the diagnosis of a true Dandy-Walker syndrome in six of the twelve cases. The remaining six proved to have either an independent posterior fossa cyst with communication of the subarachnoid space and occasionally with the ventricles; or an isolated posterior fossa cyst with no communication but with impingement on the aqueduct and on the midline structures. In six patients, a primary simple shunt procedure was performed which had to be followed by a posterior fossa craniectomy in one and by a double-shunt (ventricle and posterior fossa cyst) in another. Four patients underwent a posterior fossa craniectomy; as the primary and only procedure (2), craniectomy followed by a ventriculoperitoneal shunt (1), or an unsuccessful shunt followed by craniectomy (1). In two patients, a so-called 'double shunt' was performed as a second operation, draining simultaneously the right lateral ventricle and the posterior fossa cyst. There was no death in this series. Three children had evidence of infected shunts; they responded to adequate therapy. Four had excellent results, with normal neurological examination and normal development. Four had good results, performing in school (working) was markedly improved; two had fair results with residual neurological deficit and some mental retardation. Two patients had to be considered as poor results. Both of them, however, have shown evidence of concomitant severe congenital abnormalities of the cerebellum on the diagnostic studies. The follow-up period ranged from 1 1/2 to 7 1/2 years.", "contents": "The so-called Dandy-Walker syndrome: analysis of 12 operated cases. Twelve patients between the years of 1964 and 1971 underwent surgical treatment for the so-called Dandy-Walker syndrome at the Johns Hopkins Hospital. Five patients belonged to an early group (1-8 weeks); four patients to an intermediary group (3-20 months); and a late group consisted of three patients (3 years, 14 years and 34 years). The value of the various diagnostic studies is discussed on the basis of an analysis of the plain skull X-rays, cerebral angiograms, and combined air studies. The importance of a careful delineation of the underlying pathological condition is stressed to enable the surgeon to choose the appropriate technique. The diagnostic studies confirmed the diagnosis of a true Dandy-Walker syndrome in six of the twelve cases. The remaining six proved to have either an independent posterior fossa cyst with communication of the subarachnoid space and occasionally with the ventricles; or an isolated posterior fossa cyst with no communication but with impingement on the aqueduct and on the midline structures. In six patients, a primary simple shunt procedure was performed which had to be followed by a posterior fossa craniectomy in one and by a double-shunt (ventricle and posterior fossa cyst) in another. Four patients underwent a posterior fossa craniectomy; as the primary and only procedure (2), craniectomy followed by a ventriculoperitoneal shunt (1), or an unsuccessful shunt followed by craniectomy (1). In two patients, a so-called 'double shunt' was performed as a second operation, draining simultaneously the right lateral ventricle and the posterior fossa cyst. There was no death in this series. Three children had evidence of infected shunts; they responded to adequate therapy. Four had excellent results, with normal neurological examination and normal development. Four had good results, performing in school (working) was markedly improved; two had fair results with residual neurological deficit and some mental retardation. Two patients had to be considered as poor results. Both of them, however, have shown evidence of concomitant severe congenital abnormalities of the cerebellum on the diagnostic studies. The follow-up period ranged from 1 1/2 to 7 1/2 years."}
{"id": "PMID:1081032", "title": "Diagnosis and surgical treatment of choroid plexus papillomas.", "content": "This is a report of 22 cases of papillomas of the choroid plexus diagnosed and managed personally by the author. The angiographic diagnosis and extension of choroid plexus papillomas are described, as is the surgical technique for removing these tumors from the lateral, third, and fourth ventricles. Specific attention is given to using the angiogram as the study of choice upon which surgical technique is planned. The diagnosis of choroid plexus papilloma of the lateral ventricle is made by observing the presence of a hypertrophied anterior choroidal artery, a 'double tumor' sign with one tumor at the trigone and the other within the temporal or frontal horn, tumor stain at the trigone, and asymmetrical hydrocephalus, generally, with a shift away from the side of the larger ventricle. Posterior fossa papillomas cause symmetrical hydrocephalus and generally have tumor stain located within the midline, with the major feeding coming from either the superior cerebellar or the posteroinferior cerebellar arteries. Surgical removal of the tumor should entail an 'en bloc' resection following occlusion of the feeding and draining vessels. The tumor should be removed directly when it is in the lateral ventricle and following ventriculocerebral spinal fluid shunting when it is in the posterior fossa.", "contents": "Diagnosis and surgical treatment of choroid plexus papillomas. This is a report of 22 cases of papillomas of the choroid plexus diagnosed and managed personally by the author. The angiographic diagnosis and extension of choroid plexus papillomas are described, as is the surgical technique for removing these tumors from the lateral, third, and fourth ventricles. Specific attention is given to using the angiogram as the study of choice upon which surgical technique is planned. The diagnosis of choroid plexus papilloma of the lateral ventricle is made by observing the presence of a hypertrophied anterior choroidal artery, a 'double tumor' sign with one tumor at the trigone and the other within the temporal or frontal horn, tumor stain at the trigone, and asymmetrical hydrocephalus, generally, with a shift away from the side of the larger ventricle. Posterior fossa papillomas cause symmetrical hydrocephalus and generally have tumor stain located within the midline, with the major feeding coming from either the superior cerebellar or the posteroinferior cerebellar arteries. Surgical removal of the tumor should entail an 'en bloc' resection following occlusion of the feeding and draining vessels. The tumor should be removed directly when it is in the lateral ventricle and following ventriculocerebral spinal fluid shunting when it is in the posterior fossa."}
{"id": "PMID:1081033", "title": "[Clinical experiences with dopamine after heart surgery].", "content": "15 patients have been treated with dopamine (2.0--6.0 gamma/kg/min) in the initial phase of cardiogenic shock after cardiac surgery. Indication was a systolic blood pressure of less than 85 mmHg associated with oligurie and peripheral vasoconstriction. 12 patients survived the cardiocirculatory crisis and the early postoperative period. Dopamine alone increased the arterial blood pressure in 6 patients from 52.4 to 80.1 mmHg and the urine flow from 25.2 ml/hr to 181.2 ml/hr. To obtain an optimal perfusion pressure additional application of Noradrenalin was used in 8 patients. In these patients the urine flow rose from 9.1 ml/hr to 131 ml/hr. In one patient no reaction, neither to dopamine, nor in combination with Noradrenalin was seen. The effect of pulse rate, central venous pressure and arterial oxygen tension has been discussed. Dopamine seems to be a useful substance in surgical patients with temporary cardiac insufficiency.", "contents": "[Clinical experiences with dopamine after heart surgery]. 15 patients have been treated with dopamine (2.0--6.0 gamma/kg/min) in the initial phase of cardiogenic shock after cardiac surgery. Indication was a systolic blood pressure of less than 85 mmHg associated with oligurie and peripheral vasoconstriction. 12 patients survived the cardiocirculatory crisis and the early postoperative period. Dopamine alone increased the arterial blood pressure in 6 patients from 52.4 to 80.1 mmHg and the urine flow from 25.2 ml/hr to 181.2 ml/hr. To obtain an optimal perfusion pressure additional application of Noradrenalin was used in 8 patients. In these patients the urine flow rose from 9.1 ml/hr to 131 ml/hr. In one patient no reaction, neither to dopamine, nor in combination with Noradrenalin was seen. The effect of pulse rate, central venous pressure and arterial oxygen tension has been discussed. Dopamine seems to be a useful substance in surgical patients with temporary cardiac insufficiency."}
{"id": "PMID:1081034", "title": "Diagnostic and therapeutic considerations for fecal impaction.", "content": "During an 18-month period, 18 patients were admitted to the Beth Israel Hospital because of fecal impaction or its complications. The records of the 18 patients were reviewed to determine the presenting signs and symptoms, radiologic findings, course and etiology of fecal impaction. Prior use of drugs that slow gastrointestinal motility was found in seven cases, and seven of the 18 patients had severe neuropsychiatric illness. The presenting signs and symptoms in almost all instances were consistent with a diagnosis of intestinal obstruction. The difficulty in differentiating intestinal obstruction caused by fecal impaction from obstruction resulting from other lesions is discussed. The diagnosis of fecal impaction should be entertained only after other causes of intestinal obstruction have been excluded.", "contents": "Diagnostic and therapeutic considerations for fecal impaction. During an 18-month period, 18 patients were admitted to the Beth Israel Hospital because of fecal impaction or its complications. The records of the 18 patients were reviewed to determine the presenting signs and symptoms, radiologic findings, course and etiology of fecal impaction. Prior use of drugs that slow gastrointestinal motility was found in seven cases, and seven of the 18 patients had severe neuropsychiatric illness. The presenting signs and symptoms in almost all instances were consistent with a diagnosis of intestinal obstruction. The difficulty in differentiating intestinal obstruction caused by fecal impaction from obstruction resulting from other lesions is discussed. The diagnosis of fecal impaction should be entertained only after other causes of intestinal obstruction have been excluded."}
{"id": "PMID:1081040", "title": "[Endoscopic polypectomy of the upper gastro-intestinal tract: results and clinical features (author's transl)].", "content": "247 polyps were removed by electroresection through an endoscope (and 240 recovered) in 160 patients, aged 24-81 years. The histological substrate varied widely. Comparison of histological findings in the biopsy specimen with those of the entire polyp after polypectomy gave differing results in 41 of 87 gastric polyps. Complication rate of endoscopic polypectomy was 1.6%: postoperative bleeding occurred in three, which was treated conservatively, while in one patient ligation of a blood vessel by laparotomy became necessary. There were no perforation or lethal complications. Among three patients with hyperplasiogenic gastric polyps a \"borderline lesion\" was found in two (early carcinoma type I; adenocarcinoma). In 13 other patients with hyperplasiogenic polyps follow-up examination after one year revealed recurrence in three, absence of polyps in eight. In one patient a small gastric carcinoma (about 1 cm in diameter) was discovered four months later. It is possible that patients with hyperplasiogenic polyps are at a higher risk of cancer. Six-monthly endoscopic observation is, therefore, indicated.", "contents": "[Endoscopic polypectomy of the upper gastro-intestinal tract: results and clinical features (author's transl)]. 247 polyps were removed by electroresection through an endoscope (and 240 recovered) in 160 patients, aged 24-81 years. The histological substrate varied widely. Comparison of histological findings in the biopsy specimen with those of the entire polyp after polypectomy gave differing results in 41 of 87 gastric polyps. Complication rate of endoscopic polypectomy was 1.6%: postoperative bleeding occurred in three, which was treated conservatively, while in one patient ligation of a blood vessel by laparotomy became necessary. There were no perforation or lethal complications. Among three patients with hyperplasiogenic gastric polyps a \"borderline lesion\" was found in two (early carcinoma type I; adenocarcinoma). In 13 other patients with hyperplasiogenic polyps follow-up examination after one year revealed recurrence in three, absence of polyps in eight. In one patient a small gastric carcinoma (about 1 cm in diameter) was discovered four months later. It is possible that patients with hyperplasiogenic polyps are at a higher risk of cancer. Six-monthly endoscopic observation is, therefore, indicated."}
{"id": "PMID:1081041", "title": "[Multiple cartilaginous exostoses with polyposis of stomach and colon: a new, hereditary combination different from Gardner's syndrome (author's transl)].", "content": "Multiple cartilaginous exostoses were found in a 41-year-old man and his two sons, aged 11 and 15 years. The older boy had extensive polyposis of the sigmoid colon and gastric antrum, his brother had radiological changes suspicious of sigmoid polyposis, while there was no radiological evidence of polyposis in the father. None of them had any symptoms of polyposis. The described combination of findings suggests a separate entity from Gardner's syndrome, perhaps on the basis of a combined dominant gene inheritance. In case of multiple exostoses radiological and endoscopic examination of the gastro-intestinal tract for polyposis are indicated.", "contents": "[Multiple cartilaginous exostoses with polyposis of stomach and colon: a new, hereditary combination different from Gardner's syndrome (author's transl)]. Multiple cartilaginous exostoses were found in a 41-year-old man and his two sons, aged 11 and 15 years. The older boy had extensive polyposis of the sigmoid colon and gastric antrum, his brother had radiological changes suspicious of sigmoid polyposis, while there was no radiological evidence of polyposis in the father. None of them had any symptoms of polyposis. The described combination of findings suggests a separate entity from Gardner's syndrome, perhaps on the basis of a combined dominant gene inheritance. In case of multiple exostoses radiological and endoscopic examination of the gastro-intestinal tract for polyposis are indicated."}
{"id": "PMID:1081042", "title": "Blood glucose and plasma insulin levels during prolonged pentobarbitone anaesthesia in the rat.", "content": "Experiments were conducted on 18 hr fasted male Wistar rats at 15 minutes (short-term anaesthetised) and 5 hr (long-term anaesthetised) after the induction of anaesthesia with sodium pentobarbitone, 45 mg/kg body weight intraperitoneally. Long-term anaesthetised animals received supplementary injections of anaesthetic, 10 mg/kg body weight at hourly intervals, or as required to maintain anaesthesia. Normal basal arterial blood glucose and plasma insulin levels were well maintained during 5 hours of anaesthesia. Following an intravenous glucose challenge, plasma insulin levels and insulin-glucose ratios were slightly reduced in the long-term anaesthetised animals, but glucose tolerance was not significantly impaired. The insulin secretory capacity of pancreas pieces isolated from short-term and long-term anaesthetised animals and incubated in vitro in the absence and presence of glucose was not significantly altered. The hypoglycaemic effect of an intravenous injection of insulin was markedly diminished and the rate of elimination of insulin from the circulation was significantly increased in the long-term anaesthetised animals. These observations indicate adjustments of the glucose homeostatic mechanism during prolonged pentobarbitone anaesthesia in fasting rats.", "contents": "Blood glucose and plasma insulin levels during prolonged pentobarbitone anaesthesia in the rat. Experiments were conducted on 18 hr fasted male Wistar rats at 15 minutes (short-term anaesthetised) and 5 hr (long-term anaesthetised) after the induction of anaesthesia with sodium pentobarbitone, 45 mg/kg body weight intraperitoneally. Long-term anaesthetised animals received supplementary injections of anaesthetic, 10 mg/kg body weight at hourly intervals, or as required to maintain anaesthesia. Normal basal arterial blood glucose and plasma insulin levels were well maintained during 5 hours of anaesthesia. Following an intravenous glucose challenge, plasma insulin levels and insulin-glucose ratios were slightly reduced in the long-term anaesthetised animals, but glucose tolerance was not significantly impaired. The insulin secretory capacity of pancreas pieces isolated from short-term and long-term anaesthetised animals and incubated in vitro in the absence and presence of glucose was not significantly altered. The hypoglycaemic effect of an intravenous injection of insulin was markedly diminished and the rate of elimination of insulin from the circulation was significantly increased in the long-term anaesthetised animals. These observations indicate adjustments of the glucose homeostatic mechanism during prolonged pentobarbitone anaesthesia in fasting rats."}
{"id": "PMID:1081043", "title": "Effect of hypophysectomy on the volume and ultrastructure of zona glomerulosa in rat adrenal.", "content": "The zona glomerulosa of the rat has been examined at various times after hypophysectomy. There is no detectable change in the zona glomerulosa at 2 days after hypophysectomy. However by 7 days, the width of the glomerulosa became significantly larger in spite of an absolute decrease in the volume of the zone. No alternations in ultrastructure were discernible at this time in the glomerulosa, although giant mitochondria were seen in adjacent zona fasciculata cells. By 30 days there was a much wider zona glomerulosa and a restoration of the volume of the zone to that found in controls. Many zona glomerulosa cells were hypertrophic. The Golgi apparatus was enlarged and many vesicles were associated with peripheral cisternae. A few intramitochondrial tubules were observed in some mitochondria of the zona glomerulosa at 30 days.", "contents": "Effect of hypophysectomy on the volume and ultrastructure of zona glomerulosa in rat adrenal. The zona glomerulosa of the rat has been examined at various times after hypophysectomy. There is no detectable change in the zona glomerulosa at 2 days after hypophysectomy. However by 7 days, the width of the glomerulosa became significantly larger in spite of an absolute decrease in the volume of the zone. No alternations in ultrastructure were discernible at this time in the glomerulosa, although giant mitochondria were seen in adjacent zona fasciculata cells. By 30 days there was a much wider zona glomerulosa and a restoration of the volume of the zone to that found in controls. Many zona glomerulosa cells were hypertrophic. The Golgi apparatus was enlarged and many vesicles were associated with peripheral cisternae. A few intramitochondrial tubules were observed in some mitochondria of the zona glomerulosa at 30 days."}
{"id": "PMID:1081044", "title": "Modified technique of perfusion of isolated rat pancreas tested by insulin release after glucose administration.", "content": "A method of in vitro perfusion of isolated rat pancreas in a closed circuit is presented. The surgical procedure was slightly modified as compared to the methods published by others the superior mesenteric and coeliac arteries being fixed with tubings for the arterial inflow and the portal vein for the venous outflow. When the attached duodenum is perfused too, it is possible to study both the pancreatic and duodenal excretion. The perfusion of the pancreatic tissue was made with the aid of original equipment. The secretion of insulin into the perfusion fluid after the administration of glucose was measured by radioimmunoassay and a rapid increase of insulin level was found. This observation together with several histological aspects were considered to show the unimpaired viability of tissue.", "contents": "Modified technique of perfusion of isolated rat pancreas tested by insulin release after glucose administration. A method of in vitro perfusion of isolated rat pancreas in a closed circuit is presented. The surgical procedure was slightly modified as compared to the methods published by others the superior mesenteric and coeliac arteries being fixed with tubings for the arterial inflow and the portal vein for the venous outflow. When the attached duodenum is perfused too, it is possible to study both the pancreatic and duodenal excretion. The perfusion of the pancreatic tissue was made with the aid of original equipment. The secretion of insulin into the perfusion fluid after the administration of glucose was measured by radioimmunoassay and a rapid increase of insulin level was found. This observation together with several histological aspects were considered to show the unimpaired viability of tissue."}
{"id": "PMID:1081045", "title": "Influence of plasma hemostasis on insulin action in vitro.", "content": "The influence of various components of plasma hemostatic system on the assimilation of glucose by isolated rat hemidiaphragm in the presence of insulin was investigated. The addition of streptokinase and human plasminogen to the incubation medium decreased the rate of glucose assimilation. This effect did not occur when trasylol, an inhibitor of plasmin, was added to the assay system. The glucose assimilation by hemidiaphragms incubated in buffer with glucose and insulin was not influenced by thrombin, fibrinopeptides, fibrin degradation products, plasminogen, streptokinase or trasylol added separately to the assay system. The mechanism of the influence of plasmin on insulin action was partly elucidated in further experiments. Thus, less 125I-insulin was bound by isolated fat cells preincubated with plasmin than by control cells.", "contents": "Influence of plasma hemostasis on insulin action in vitro. The influence of various components of plasma hemostatic system on the assimilation of glucose by isolated rat hemidiaphragm in the presence of insulin was investigated. The addition of streptokinase and human plasminogen to the incubation medium decreased the rate of glucose assimilation. This effect did not occur when trasylol, an inhibitor of plasmin, was added to the assay system. The glucose assimilation by hemidiaphragms incubated in buffer with glucose and insulin was not influenced by thrombin, fibrinopeptides, fibrin degradation products, plasminogen, streptokinase or trasylol added separately to the assay system. The mechanism of the influence of plasmin on insulin action was partly elucidated in further experiments. Thus, less 125I-insulin was bound by isolated fat cells preincubated with plasmin than by control cells."}
{"id": "PMID:1081046", "title": "Effect of epinephrine on adipose tissue metabolism in goats.", "content": "In adipose tissue of large omentum the release of non-esterified fatty acids (NEFA) was stimulated by epinephrine at concentration as low as 0.25 mug/ml. The stimulation increased sharply within the range of 1-10 mug epinephrine per ml and reached a maximum at 10 mug/ml. The incorporation of glucose and acetate into fatty acids and that of glucose into glycerol decreased with increasing concentration of epinephrine in the incubation medium. In the absence  of glucose and acetate in the incubation medium, hydroxybutyrate at concentrations within 0.96-96 mM decreased significantly either the spontaneous lipolysis or that stimulated by epinephrine. In the presence of 2.5 mM glucose and 5 mM acetate in the incubation medium, beta-hydroxybutyrate did not significantly inhibit the mobilization of NEFA stimulated by epinephrine even at a concentration of 96 mM. In contrast, it accentuated the effect of epinephrine on the decrease of synthesis of fatty acids de novo and on the increase of reesterification of fatty acids. These results indicate that glucose and acetate may counteract the inhibitory effect of beta-hydroxybutyrate on lipolysis.", "contents": "Effect of epinephrine on adipose tissue metabolism in goats. In adipose tissue of large omentum the release of non-esterified fatty acids (NEFA) was stimulated by epinephrine at concentration as low as 0.25 mug/ml. The stimulation increased sharply within the range of 1-10 mug epinephrine per ml and reached a maximum at 10 mug/ml. The incorporation of glucose and acetate into fatty acids and that of glucose into glycerol decreased with increasing concentration of epinephrine in the incubation medium. In the absence  of glucose and acetate in the incubation medium, hydroxybutyrate at concentrations within 0.96-96 mM decreased significantly either the spontaneous lipolysis or that stimulated by epinephrine. In the presence of 2.5 mM glucose and 5 mM acetate in the incubation medium, beta-hydroxybutyrate did not significantly inhibit the mobilization of NEFA stimulated by epinephrine even at a concentration of 96 mM. In contrast, it accentuated the effect of epinephrine on the decrease of synthesis of fatty acids de novo and on the increase of reesterification of fatty acids. These results indicate that glucose and acetate may counteract the inhibitory effect of beta-hydroxybutyrate on lipolysis."}
{"id": "PMID:1081047", "title": "Gomori-positive cells in rat thymus: Lack of evidence on endocrine function.", "content": "Large Gomori-positive cells were observed in rat thymus after hormonal induction (e. g. after treatment with oxytocin or hydrocortisone). In contrast to some findings by others, no evidence on endocrine function of these cells was found and they were identified as macrophages containing, in their cytoplasm, great amount of phagocytized material.", "contents": "Gomori-positive cells in rat thymus: Lack of evidence on endocrine function. Large Gomori-positive cells were observed in rat thymus after hormonal induction (e. g. after treatment with oxytocin or hydrocortisone). In contrast to some findings by others, no evidence on endocrine function of these cells was found and they were identified as macrophages containing, in their cytoplasm, great amount of phagocytized material."}
{"id": "PMID:1081048", "title": "Thyroid state and brain monoamine metabolism.", "content": "The rates at which rat brain synthesizes catecholamines and serotonin were estimated by measuring the accumulation of DOPA and 5-hydroxytryptophan (5-HTP) 45 min after ip administration of the decarboxylase inhibitor RO4-4602 (800 mg/kg BW). Following thyroparathy-roidectomy, hypothyroid rats showed a decreased accumulation of both precursor amino acids. On the other hand, hyperthyroidism (caused by administering 15 mug T4/100 g BW for 25 days) accelerated the accumulation of catecholamines and serotonin. The accumulation of 5-HTP correlated closely with brain tryptophan concentration in all treatment groups; DOPA accumulation, however, did not similarly correspond to brain tyrosine levels.", "contents": "Thyroid state and brain monoamine metabolism. The rates at which rat brain synthesizes catecholamines and serotonin were estimated by measuring the accumulation of DOPA and 5-hydroxytryptophan (5-HTP) 45 min after ip administration of the decarboxylase inhibitor RO4-4602 (800 mg/kg BW). Following thyroparathy-roidectomy, hypothyroid rats showed a decreased accumulation of both precursor amino acids. On the other hand, hyperthyroidism (caused by administering 15 mug T4/100 g BW for 25 days) accelerated the accumulation of catecholamines and serotonin. The accumulation of 5-HTP correlated closely with brain tryptophan concentration in all treatment groups; DOPA accumulation, however, did not similarly correspond to brain tyrosine levels."}
{"id": "PMID:1081049", "title": "The ACTH-releasing hormone of the hypothalamus requires a co-factor.", "content": "Gel filtration of an extract of rat median eminence tissue in 0.1 N HCl on Sephadex G-25 separated two peaks, both with feeble ACTH-releasing activity. Full activity of the extract was regained when both peaks were recombined. This observation suggests that the ACTH-releasing hormone of the hypothalamus requires a co-factor for activity.", "contents": "The ACTH-releasing hormone of the hypothalamus requires a co-factor. Gel filtration of an extract of rat median eminence tissue in 0.1 N HCl on Sephadex G-25 separated two peaks, both with feeble ACTH-releasing activity. Full activity of the extract was regained when both peaks were recombined. This observation suggests that the ACTH-releasing hormone of the hypothalamus requires a co-factor for activity."}
{"id": "PMID:1081050", "title": "Trypsin-kallikrein isoinhibitor K (type Kunitz) from snails (Helix pomatia). Purification and characterization.", "content": "A basic proteinase inhibitor, isoinhibitor K, was purified by SE-Sephadex C-25 column chromatography from the mixture of acid-stable and heat-stable isoinhibitors of the snail (Helix pomatia). Isoinhibitor K is homogeneous in polyacrylamide gel, cellulose acetate and polyacrylamide-dodecylsulfate electrophoresis. From the electrophoretic mobility in dodecylsulfate-polyacrylamide gel and apparent molecular weight of 6500 +/- 200 was estimated. From the amino acid composition the inhibitor consists of 58 amino acid residues. It contains three disulfide bridges, a C-terminal valine and a lysine residue at the reactive site. Isoinhibitor K inhibits the enzymes: bovine trypsin and chymotrypsin, porcine plasmin and pancreatic kallikrein, the trypsin-like component of Streptomyces griseus proteinase-pronase E, and fungi proteinase K from Tritirachium album Limber, which is only inhibited very slightly in contrast to the effect of the mixture of isoinhibitors. The inhibitory effect of isoinhibitor K against these enzymes is compared to that of the mixture or of other isoinhibitors. The following enzymes are not inhibited by isoinhibitor K: Aspergillus proteinase P and alkaline bacillus proteinase 2231 (R\u00f6hm), which both are inhibited by the mixture of isoinhibitors. Porcine elastase, bacterial proteinase N (M) (R\u00f6hm), and a trypsin-like proteinase from wheat are not inhibited, porcine acrosin and porcine serum kallikrein only to a very minor extent by the mixture of isoinhibitors. Reactive-site peptide-bond cleavage during inhibition could not be detected. Thus, the inhibitory behaviour is just as broad in specificity and as unusual as that of the trypsin-kallikrein inhibitor (Kunitz) from bovine organs. The N-terminus is blocked by pyroglutamic acid. Isoinhibitor K is the main component of the isoinhibitors secreted into the mucus and amounts to 35-40% of the mixture.", "contents": "Trypsin-kallikrein isoinhibitor K (type Kunitz) from snails (Helix pomatia). Purification and characterization. A basic proteinase inhibitor, isoinhibitor K, was purified by SE-Sephadex C-25 column chromatography from the mixture of acid-stable and heat-stable isoinhibitors of the snail (Helix pomatia). Isoinhibitor K is homogeneous in polyacrylamide gel, cellulose acetate and polyacrylamide-dodecylsulfate electrophoresis. From the electrophoretic mobility in dodecylsulfate-polyacrylamide gel and apparent molecular weight of 6500 +/- 200 was estimated. From the amino acid composition the inhibitor consists of 58 amino acid residues. It contains three disulfide bridges, a C-terminal valine and a lysine residue at the reactive site. Isoinhibitor K inhibits the enzymes: bovine trypsin and chymotrypsin, porcine plasmin and pancreatic kallikrein, the trypsin-like component of Streptomyces griseus proteinase-pronase E, and fungi proteinase K from Tritirachium album Limber, which is only inhibited very slightly in contrast to the effect of the mixture of isoinhibitors. The inhibitory effect of isoinhibitor K against these enzymes is compared to that of the mixture or of other isoinhibitors. The following enzymes are not inhibited by isoinhibitor K: Aspergillus proteinase P and alkaline bacillus proteinase 2231 (R\u00f6hm), which both are inhibited by the mixture of isoinhibitors. Porcine elastase, bacterial proteinase N (M) (R\u00f6hm), and a trypsin-like proteinase from wheat are not inhibited, porcine acrosin and porcine serum kallikrein only to a very minor extent by the mixture of isoinhibitors. Reactive-site peptide-bond cleavage during inhibition could not be detected. Thus, the inhibitory behaviour is just as broad in specificity and as unusual as that of the trypsin-kallikrein inhibitor (Kunitz) from bovine organs. The N-terminus is blocked by pyroglutamic acid. Isoinhibitor K is the main component of the isoinhibitors secreted into the mucus and amounts to 35-40% of the mixture."}
{"id": "PMID:1081052", "title": "Trigeminal representations of the masticatory and extraocular proprioceptors as revealed by horseradish peroxidase retrograde transport.", "content": "Horseradish peroxidase has been injected in the masticatory and extraocular muscles in newborn and adult cats to identify the cells of origin for the muscle endings. Labeled motoneurons in the nuclei of the III, IV, V and VI nerves have been observed. They are the parent cells of the motor terminals taking up the enzyme in the muscle injected. Labeled ganglionic cells have been found scattered all along the ipsilateral mesencephalic nucleus of the V nerve after injection of both the jaw closing and the jaw opening muscles. Labeled cells have also been found in the ipsilateral caudal part of the same nucleus after injection of the extraocular muscles. These results are interpreted as due to enzyme uptake by the sensory endings of the muscle studied. Moreover cell bodies in the semilunar ganglion were found marked for both groups of muscles injected showing a second ganglionic representation for the sensory endings.", "contents": "Trigeminal representations of the masticatory and extraocular proprioceptors as revealed by horseradish peroxidase retrograde transport. Horseradish peroxidase has been injected in the masticatory and extraocular muscles in newborn and adult cats to identify the cells of origin for the muscle endings. Labeled motoneurons in the nuclei of the III, IV, V and VI nerves have been observed. They are the parent cells of the motor terminals taking up the enzyme in the muscle injected. Labeled ganglionic cells have been found scattered all along the ipsilateral mesencephalic nucleus of the V nerve after injection of both the jaw closing and the jaw opening muscles. Labeled cells have also been found in the ipsilateral caudal part of the same nucleus after injection of the extraocular muscles. These results are interpreted as due to enzyme uptake by the sensory endings of the muscle studied. Moreover cell bodies in the semilunar ganglion were found marked for both groups of muscles injected showing a second ganglionic representation for the sensory endings."}
{"id": "PMID:1081054", "title": "Age-dependence of spontaneous delayed hypersensitivity to DNA, antinuclear antibody production and development of glomerulonephritis in NZB mice.", "content": "In NZB mice of different ages (2, 4, 8, 12, and 16 months) the positivity of the migration inhibition test performed with the ds-DNA in spleen explants was compared with the level of immunofluorescent antinuclear antibodies and the incidence of glomerulonephritis. The positivity of the MIT was apparent at 4 months of age, rose with ageing and decreased after 12 months of age. Production of ANA began later, mostly at 8 months of age, increased progressively with ageing, especially in female mice, and was followed by the development of glomerulonephritis which reached its peak at 16 months of age.", "contents": "Age-dependence of spontaneous delayed hypersensitivity to DNA, antinuclear antibody production and development of glomerulonephritis in NZB mice. In NZB mice of different ages (2, 4, 8, 12, and 16 months) the positivity of the migration inhibition test performed with the ds-DNA in spleen explants was compared with the level of immunofluorescent antinuclear antibodies and the incidence of glomerulonephritis. The positivity of the MIT was apparent at 4 months of age, rose with ageing and decreased after 12 months of age. Production of ANA began later, mostly at 8 months of age, increased progressively with ageing, especially in female mice, and was followed by the development of glomerulonephritis which reached its peak at 16 months of age."}
{"id": "PMID:1081061", "title": "On the role of the reticular-epithelial complex in transplantation of the thymus.", "content": "Transplantation of the neonatal thymus, into young, adult hosts, resulted in massive cell death of graft cortical lymphoid tissue with apparent selective survival of the reticular-epithelial cells. The central area of the graft was progressively cleared of cell debris and the characteristic thymic architecture restored within fourteen days of grafting. Evidence obtained from the regeneration of different-sized transplants suggested that the size and shape attained by the regenerated graft was closely related to the size and shape of the donor tissue. When donor rat thymuses were transplanted in Millipore chambers, the lymphocyte population did not reappear and after seven days only reticular-epithelial cells remained, retaining their normal appearance. However, when these thymic remnants were removed from the chambers and transplanted into secondary hosts, the thymus regenerated normally, suggesting that the lymphocytes in the regenerated gland were derived from the host. Thymic remnants after cortisol treatment of donors also formed distinct organs after grafting despite the fact that they contained few donor lymphocytes. From the differential effects of cortisol on host and transplanted thymus and the different growth characteristics of transplants it appears that transplants differ in their growth/involution control system from the host thymus.", "contents": "On the role of the reticular-epithelial complex in transplantation of the thymus. Transplantation of the neonatal thymus, into young, adult hosts, resulted in massive cell death of graft cortical lymphoid tissue with apparent selective survival of the reticular-epithelial cells. The central area of the graft was progressively cleared of cell debris and the characteristic thymic architecture restored within fourteen days of grafting. Evidence obtained from the regeneration of different-sized transplants suggested that the size and shape attained by the regenerated graft was closely related to the size and shape of the donor tissue. When donor rat thymuses were transplanted in Millipore chambers, the lymphocyte population did not reappear and after seven days only reticular-epithelial cells remained, retaining their normal appearance. However, when these thymic remnants were removed from the chambers and transplanted into secondary hosts, the thymus regenerated normally, suggesting that the lymphocytes in the regenerated gland were derived from the host. Thymic remnants after cortisol treatment of donors also formed distinct organs after grafting despite the fact that they contained few donor lymphocytes. From the differential effects of cortisol on host and transplanted thymus and the different growth characteristics of transplants it appears that transplants differ in their growth/involution control system from the host thymus."}
{"id": "PMID:1081062", "title": "Resection of left ventricular aneurysm. Report of 277 patients.", "content": "The prognosis of patients with LVA (left ventricular aneurysm) treated medically has been uniformly poor. Surgical resection of the ventricular aneurysm has been the treatment of choice at the Texas Heart Institute since 1958. A more accurate evaluation of patients with LVA by selective coronary arteriography has demonstrated significant associated CAD in over 75 percent of these patients. Since 1969, 125 patients with at lease one vessel suitable for bypass (group II) have undergone single, double or triple ACB in association with LVA resection; and 51 patients (group III) without significant conc-omitant CAD underwent LVA resection alone. To evaluate the efficacy of combined ACB and LVA resection, patients in group II and group III were compared to a third group of 101 patients who underwent LVA resection alone from 1958 to 1969 (group I). Operative mortality was higher in group I (19.8%) as compared to group II (12.8%) and group III (9.5%). Higher mortality was found to be related to a coronary artery score above 8.9, presence of left main coronary lesion, posterior or inferior location of the LVA, severe concomitant mitral valve insufficiency and incomplete revascularization of the remaining ventricular myocardium after LVA resection. Lower mortality and improved long-term results found in groups II and III as compared to group I appear to justify our choice of a \"complete operation\" in patients with LVA.", "contents": "Resection of left ventricular aneurysm. Report of 277 patients. The prognosis of patients with LVA (left ventricular aneurysm) treated medically has been uniformly poor. Surgical resection of the ventricular aneurysm has been the treatment of choice at the Texas Heart Institute since 1958. A more accurate evaluation of patients with LVA by selective coronary arteriography has demonstrated significant associated CAD in over 75 percent of these patients. Since 1969, 125 patients with at lease one vessel suitable for bypass (group II) have undergone single, double or triple ACB in association with LVA resection; and 51 patients (group III) without significant conc-omitant CAD underwent LVA resection alone. To evaluate the efficacy of combined ACB and LVA resection, patients in group II and group III were compared to a third group of 101 patients who underwent LVA resection alone from 1958 to 1969 (group I). Operative mortality was higher in group I (19.8%) as compared to group II (12.8%) and group III (9.5%). Higher mortality was found to be related to a coronary artery score above 8.9, presence of left main coronary lesion, posterior or inferior location of the LVA, severe concomitant mitral valve insufficiency and incomplete revascularization of the remaining ventricular myocardium after LVA resection. Lower mortality and improved long-term results found in groups II and III as compared to group I appear to justify our choice of a \"complete operation\" in patients with LVA."}
{"id": "PMID:1081063", "title": "[Aorto-coronary bypass. On 200 cases surgically treated (author's transl].", "content": "200 patients underwent bypass graft for treatment of severe coronary vessel disease. The bypass vein grafts were 316; single grafts in 100 cases, double in 85, triple in 14 and in 1 case a patient received 4 grafts. In 57 cases a previous coronary endarterectomy was performed. The flow in the graft at the end of the operation was more than 40 cc/min. in 80% of cases. 22 patients died. Postoperative myocardial infarction occurred in 14% of the patients. The mortality was higher in the preinfarction angina group, and less in the chronic angina group without concomitant surgery (7,2%) and is statistically in direct relation to the functional condition of the left ventricle (2,8% in patients with end diastolic left ventricular pressure less than 20 mmHg and without akinetic areas). Relief of angina was observed in 87% of the surviving patients. Postoperative coronarography, catheterism and left ventriculography was performed in 46 patients. Only 2 of the 15 cases with postoperative angina had the graft patent. Significant improvement of the left ventricular function with disappearence or regression of akinetic areas was observed in 9 patients. Our experience indicates that the value of this operation cannot be entirely known at the present time.", "contents": "[Aorto-coronary bypass. On 200 cases surgically treated (author's transl]. 200 patients underwent bypass graft for treatment of severe coronary vessel disease. The bypass vein grafts were 316; single grafts in 100 cases, double in 85, triple in 14 and in 1 case a patient received 4 grafts. In 57 cases a previous coronary endarterectomy was performed. The flow in the graft at the end of the operation was more than 40 cc/min. in 80% of cases. 22 patients died. Postoperative myocardial infarction occurred in 14% of the patients. The mortality was higher in the preinfarction angina group, and less in the chronic angina group without concomitant surgery (7,2%) and is statistically in direct relation to the functional condition of the left ventricle (2,8% in patients with end diastolic left ventricular pressure less than 20 mmHg and without akinetic areas). Relief of angina was observed in 87% of the surviving patients. Postoperative coronarography, catheterism and left ventriculography was performed in 46 patients. Only 2 of the 15 cases with postoperative angina had the graft patent. Significant improvement of the left ventricular function with disappearence or regression of akinetic areas was observed in 9 patients. Our experience indicates that the value of this operation cannot be entirely known at the present time."}
{"id": "PMID:1081068", "title": "[Phytomitogen-induced lymphocyte transformation - standardization and diagnostic value (author's transl)].", "content": "Lymphocytes can transform into blasts. This basic biological property is the prerequisite for many reactions of the cellular immune system. Therefore, lymphocyte transformation tests appear to be a valuable diagnostic approach to define primary and secondary immundeficiency associated with different diseases. The validity of the test system is based on a standardization wich can be achieved sufficiently with regard to culture conditions and measurement of DNA-synthesis. But for phytomitogen-induced lymphocyte transformation a cooperative system of lymphocytes and macrophages is necessary. The results are influenced to a high degree by different cell separation procedures. Therefore, the interpretation of abnormal lymphocyte transformation data, induced unspecifically mitogens, has to take into account alteration of lymphocyte function, different T-/B-cell ratios and changing counts and helper effects of macrophages in order to define a defective lymphocyte transformation more precisely.", "contents": "[Phytomitogen-induced lymphocyte transformation - standardization and diagnostic value (author's transl)]. Lymphocytes can transform into blasts. This basic biological property is the prerequisite for many reactions of the cellular immune system. Therefore, lymphocyte transformation tests appear to be a valuable diagnostic approach to define primary and secondary immundeficiency associated with different diseases. The validity of the test system is based on a standardization wich can be achieved sufficiently with regard to culture conditions and measurement of DNA-synthesis. But for phytomitogen-induced lymphocyte transformation a cooperative system of lymphocytes and macrophages is necessary. The results are influenced to a high degree by different cell separation procedures. Therefore, the interpretation of abnormal lymphocyte transformation data, induced unspecifically mitogens, has to take into account alteration of lymphocyte function, different T-/B-cell ratios and changing counts and helper effects of macrophages in order to define a defective lymphocyte transformation more precisely."}
{"id": "PMID:1081069", "title": "[Analysis and differentiation of cytergic immune reactions: tuberculine type and cutaneous basophilic hypersensitivity (author's transl)].", "content": "The classic delayed tuberculine reaction represents a T-cell mediated immune reaction, detectable already before the formation of antibodies in the course of proteine sensitization. Myocobacteria stimulate and enhance this reaction. The skin test reaction needs the whole antigen for induction. The reaction starts 6 to 8 hours later, shows maximal development 24 to 28 (-72) hours later and is characterized by inflammation, induration, sometimes with hemorrhagies, necroses and ulceration, a macrophage infiltration is typical in the histological picture. During the reaction between antigen and sensitized T-cells mediators are set free which act by the way of unspecific stimulation on T-, B-lymphocytes and macrophages, otherwise they may inhibit macrophage migration. The delayed reaction is active in many infectious diseases and in the rejection of transplants and tumours. The cutaneous basophilic hypersensitivity (Jones-Mote) is a T-cell mediated immune reaction, detectable even before the classic delayed reaction after sensitization with tiny up to large doses of proteines. The skin reaction needs the whole antigen for induction, is visible 2 to 4 hours later in form of an inflammation with slight swelling and reaches maximal development 12 to 24 hours later without hemorrhagies or necroses. The infiltration consists mainly of basophilic leucocytes. There exists a weaker avidity of the T-cells of the cutaneous basophilic hypersensitivity compared to the T-cells of the delayed reaction or to the amount of antibodies. Therefore this reaction is easily suppressed by other immune reactions. In contactdermatitis and vaccine virus infections of the guinea pig the cutaneous basophilic hypersensitivity consists for weeks and months because under these circumstances other immune reactions do not compete. The differentiation of the two reactions shall develop to a routine laboratory procedure due to their therapeutic consequences in the immune diagnostic field.", "contents": "[Analysis and differentiation of cytergic immune reactions: tuberculine type and cutaneous basophilic hypersensitivity (author's transl)]. The classic delayed tuberculine reaction represents a T-cell mediated immune reaction, detectable already before the formation of antibodies in the course of proteine sensitization. Myocobacteria stimulate and enhance this reaction. The skin test reaction needs the whole antigen for induction. The reaction starts 6 to 8 hours later, shows maximal development 24 to 28 (-72) hours later and is characterized by inflammation, induration, sometimes with hemorrhagies, necroses and ulceration, a macrophage infiltration is typical in the histological picture. During the reaction between antigen and sensitized T-cells mediators are set free which act by the way of unspecific stimulation on T-, B-lymphocytes and macrophages, otherwise they may inhibit macrophage migration. The delayed reaction is active in many infectious diseases and in the rejection of transplants and tumours. The cutaneous basophilic hypersensitivity (Jones-Mote) is a T-cell mediated immune reaction, detectable even before the classic delayed reaction after sensitization with tiny up to large doses of proteines. The skin reaction needs the whole antigen for induction, is visible 2 to 4 hours later in form of an inflammation with slight swelling and reaches maximal development 12 to 24 hours later without hemorrhagies or necroses. The infiltration consists mainly of basophilic leucocytes. There exists a weaker avidity of the T-cells of the cutaneous basophilic hypersensitivity compared to the T-cells of the delayed reaction or to the amount of antibodies. Therefore this reaction is easily suppressed by other immune reactions. In contactdermatitis and vaccine virus infections of the guinea pig the cutaneous basophilic hypersensitivity consists for weeks and months because under these circumstances other immune reactions do not compete. The differentiation of the two reactions shall develop to a routine laboratory procedure due to their therapeutic consequences in the immune diagnostic field."}
{"id": "PMID:1081070", "title": "[Humoral immune phenomena in acute and chronic viral hepatitis (author's transl)].", "content": "During HBAg positive acute viral hepatitis transitory antibodies of a low titer against smooth muscle, vascular endothelium, nuclei, and liver cell membrane antigens (polygonal pattern of immunofluorescence) occur. In HBAg positive chronic aggressive hepatitis, sometimes antibodies against nuclei or smooth muscle are associated with HBAg. Their occurrence heralds an unfavorable prognosis. High titer antibodies against nuclei and smooth muscle - a typical serology finding in lupoid chronic aggressive hepatitis - and mitochondrial antibodies have not been observed in a HBAg positive hepatitis. Subdividing chronic hepatitis in six different groups is possible with HBAg and antibodies. The predominance of histocompatability antigen HLA 8 in lupoid hepatitis and a lack of this leukocyte antigen in HGAg positive chronic aggressive hepatitis suggests that in the various forms of hepatitis, a different susceptability and capability for immune response towards microorganism is reflected. It is postulated that for course and outcome of hepatitis, the type of immune reaction plays a decisive role.", "contents": "[Humoral immune phenomena in acute and chronic viral hepatitis (author's transl)]. During HBAg positive acute viral hepatitis transitory antibodies of a low titer against smooth muscle, vascular endothelium, nuclei, and liver cell membrane antigens (polygonal pattern of immunofluorescence) occur. In HBAg positive chronic aggressive hepatitis, sometimes antibodies against nuclei or smooth muscle are associated with HBAg. Their occurrence heralds an unfavorable prognosis. High titer antibodies against nuclei and smooth muscle - a typical serology finding in lupoid chronic aggressive hepatitis - and mitochondrial antibodies have not been observed in a HBAg positive hepatitis. Subdividing chronic hepatitis in six different groups is possible with HBAg and antibodies. The predominance of histocompatability antigen HLA 8 in lupoid hepatitis and a lack of this leukocyte antigen in HGAg positive chronic aggressive hepatitis suggests that in the various forms of hepatitis, a different susceptability and capability for immune response towards microorganism is reflected. It is postulated that for course and outcome of hepatitis, the type of immune reaction plays a decisive role."}
{"id": "PMID:1081075", "title": "Rosette formation with mouse erythrocytes: probable marker for human B lymphocytes.", "content": "Studies of peripheral blood lymphocytes were performed in 10 healthy control subjects and 15 patients with hematological disorders. Spontaneous formation of mouse red blood cell-forming rosettes (MRFC) were observed with 8.1% of lymphocytes in control subjects (range 4.2--12.0%), while rosette formation with sheep red blood cells (SRFC) and complement-coated sheep erythrocyte (EAC rosettes) was present in 69.2 and 10.7%, respectively. Mouse rosette-forming cells were increased in parallel fashion with EAC rosettes in five patients with chronic lymphocytic leukemia suggesting that mouse red blood cells (MRBC) may serve as a B cell marker. A single patient with a lymphoma associated with increased peripheral SRFC did not have a concomitant rise of MRFC. It appears likely that MRBC will provide another detection marker for B lymphocytes by spontaneous rosette formation.", "contents": "Rosette formation with mouse erythrocytes: probable marker for human B lymphocytes. Studies of peripheral blood lymphocytes were performed in 10 healthy control subjects and 15 patients with hematological disorders. Spontaneous formation of mouse red blood cell-forming rosettes (MRFC) were observed with 8.1% of lymphocytes in control subjects (range 4.2--12.0%), while rosette formation with sheep red blood cells (SRFC) and complement-coated sheep erythrocyte (EAC rosettes) was present in 69.2 and 10.7%, respectively. Mouse rosette-forming cells were increased in parallel fashion with EAC rosettes in five patients with chronic lymphocytic leukemia suggesting that mouse red blood cells (MRBC) may serve as a B cell marker. A single patient with a lymphoma associated with increased peripheral SRFC did not have a concomitant rise of MRFC. It appears likely that MRBC will provide another detection marker for B lymphocytes by spontaneous rosette formation."}
{"id": "PMID:1081076", "title": "Bacteriophage MS-2 in the immune response.", "content": "Several aspects of the immune response to bacteriophage MS-2 were studied. In thymectomized, irradiated and bone marrow-reconstituted (TXBM) mice, the response was normal when a high dose of antigen was used. With a 50-fold lower dose of MS-2, the response was impaired, indicating a T-cell involvement in antibody formation. More evidence for the (partial) T-cell dependence of MS-2 was obtained from experiments with anti-thymocyte serum or cyclophosphamide-treated mice. (3H)-thymidine incorporation experiments demonstrated that both B and T cells were active upon in vitro stimulation with MS-2. The dose of MS-2 which was able to induce a normal response in TXBM mice, proved to be optimal for both sensitization and elicitation of a DH. It is concluded that MS-2 is a thymus-dependent antigen which is only thymus independent in high doses.", "contents": "Bacteriophage MS-2 in the immune response. Several aspects of the immune response to bacteriophage MS-2 were studied. In thymectomized, irradiated and bone marrow-reconstituted (TXBM) mice, the response was normal when a high dose of antigen was used. With a 50-fold lower dose of MS-2, the response was impaired, indicating a T-cell involvement in antibody formation. More evidence for the (partial) T-cell dependence of MS-2 was obtained from experiments with anti-thymocyte serum or cyclophosphamide-treated mice. (3H)-thymidine incorporation experiments demonstrated that both B and T cells were active upon in vitro stimulation with MS-2. The dose of MS-2 which was able to induce a normal response in TXBM mice, proved to be optimal for both sensitization and elicitation of a DH. It is concluded that MS-2 is a thymus-dependent antigen which is only thymus independent in high doses."}
{"id": "PMID:1081077", "title": "Carrier effect by Nippostrongylus brasiliensis infection on rat antihapten IgE antibody response.", "content": "Nippostrongylus brasiliensis infection was found to be highly effective in inducing a carrier-specific enhancing effect on primary and secondary antihapten IgE antibody response in the rat, when animals were immunized i.p. with 10 mug of dinitrophenylated N. brasiliensis protein (DNP-Nb) plus 10 mg Al(OH)3 2 weeks after the infection. The carrier effect by the infection was much greater than that obtained by any other supplementary immunization with the carrier (Nb) plus adjuvant, so far examined, in terms of the IgE antibody response. The results, together with our previous observation in the mouse, provide an explanation for the reason why antiworm IgE antibodies are so easily detectable in helminth infections.", "contents": "Carrier effect by Nippostrongylus brasiliensis infection on rat antihapten IgE antibody response. Nippostrongylus brasiliensis infection was found to be highly effective in inducing a carrier-specific enhancing effect on primary and secondary antihapten IgE antibody response in the rat, when animals were immunized i.p. with 10 mug of dinitrophenylated N. brasiliensis protein (DNP-Nb) plus 10 mg Al(OH)3 2 weeks after the infection. The carrier effect by the infection was much greater than that obtained by any other supplementary immunization with the carrier (Nb) plus adjuvant, so far examined, in terms of the IgE antibody response. The results, together with our previous observation in the mouse, provide an explanation for the reason why antiworm IgE antibodies are so easily detectable in helminth infections."}
{"id": "PMID:1081078", "title": "Host responses within solid tumors: non-thymus-derived specific cytotoxic cells within a murine mammary adenocarcinoma.", "content": "The nature of host-derived, specifically cytotoxic cells infiltrating solid murine mammary adenocarcinomas (line T1699) was investigated. Cell suspensions obtained from enzymatically dispersed tumors were separated by sedimentation velocity. The host cell fraction was heterogeneous and contained T-lymphocytes, non-phagocytic cells bearing Fc receptors, eosinophils and monocytes. Host cells equivalent in size to small lymphocytes and bearing Fc receptors were found to be the predominant cell type responsible for colony inhibition. These colony-inhibiting cells were insensitive to lysis with either anti-theta or anti-IgG serum and complement; and they were specific both in their induction and in their mediation and in their mediation of cytotoxicity. Host cells made up the predominant population in spontaneously regressive tumors but only a minor component in progressive tumors, and they were totally absent from progressive tumors in X-irradiated animals.", "contents": "Host responses within solid tumors: non-thymus-derived specific cytotoxic cells within a murine mammary adenocarcinoma. The nature of host-derived, specifically cytotoxic cells infiltrating solid murine mammary adenocarcinomas (line T1699) was investigated. Cell suspensions obtained from enzymatically dispersed tumors were separated by sedimentation velocity. The host cell fraction was heterogeneous and contained T-lymphocytes, non-phagocytic cells bearing Fc receptors, eosinophils and monocytes. Host cells equivalent in size to small lymphocytes and bearing Fc receptors were found to be the predominant cell type responsible for colony inhibition. These colony-inhibiting cells were insensitive to lysis with either anti-theta or anti-IgG serum and complement; and they were specific both in their induction and in their mediation and in their mediation of cytotoxicity. Host cells made up the predominant population in spontaneously regressive tumors but only a minor component in progressive tumors, and they were totally absent from progressive tumors in X-irradiated animals."}
{"id": "PMID:1081079", "title": "The effect of radiation on processing of nuclear RNA and chromatin ribonuclease activity in rat liver and thymus.", "content": "The effect of radiation on the kinetics of nuclear RNA degradation was studied during actinomycin chase. The intranuclear breakdown of RNA in thymus was inhibited for the first 30-120 min after 800 R irradiation of rats. In liver the degradation of nuclear RNA was unchanged for 60 min after irradiation. By the second hour, the breakdown of the rRNA precursor accelerated and the processing of D-RNA slowed down. Rat thymus and liver chromatin showed RNAase activity with two optimal pHvalues- in the acidic (pH 5-0-5-5) and weakly alkaline (pH 7-5) regions. The activity of the acidic RNAase of thymus (but not the liver) chromatin fell after 5-20 kR irradiation in vitro. The activity of the alkaline RNAase did not change under these conditions. The data indicate that a fall in activity of the acidic RNAase in irradiated thymus chromatin may be related to disturbance in the enzyme-inhibitor interaction. A possible contribution of the chromatin acidic RNAase to the processing of nuclear RNA in control and after irradiation is discussed.", "contents": "The effect of radiation on processing of nuclear RNA and chromatin ribonuclease activity in rat liver and thymus. The effect of radiation on the kinetics of nuclear RNA degradation was studied during actinomycin chase. The intranuclear breakdown of RNA in thymus was inhibited for the first 30-120 min after 800 R irradiation of rats. In liver the degradation of nuclear RNA was unchanged for 60 min after irradiation. By the second hour, the breakdown of the rRNA precursor accelerated and the processing of D-RNA slowed down. Rat thymus and liver chromatin showed RNAase activity with two optimal pHvalues- in the acidic (pH 5-0-5-5) and weakly alkaline (pH 7-5) regions. The activity of the acidic RNAase of thymus (but not the liver) chromatin fell after 5-20 kR irradiation in vitro. The activity of the alkaline RNAase did not change under these conditions. The data indicate that a fall in activity of the acidic RNAase in irradiated thymus chromatin may be related to disturbance in the enzyme-inhibitor interaction. A possible contribution of the chromatin acidic RNAase to the processing of nuclear RNA in control and after irradiation is discussed."}
{"id": "PMID:1081080", "title": "Energy requirements for damaging DNA molecules. III. The mechanisms of inactivation of bacteriophage chiX 174 DNA by vacuum ultra-violet radiation.", "content": "Strand break formation and biological inactivation of infectious DNA of bacteriophage phi chi 174 exposed to vacuum-ultra-violet radiation of 4-9 to 21-2 eV quantum energy is investigated. At 21-2 eV as many as 84 per cent of the DNA molecules are inactivated by breaks whereas breaks do not contribute to inactivation at 4-9 eV. The quantum yield of break formation increases from 1-7 X 10(-5) (4-9 EV) to 0-55 (21-2 eV) and shows a dependence on energy similar to that of electron emission (due to ionization) above 8 eV. The mechanisms leading to break formation and inactivation are discussed taking the absorption spectrum of DNA in the vacuum-ultra-violet region into consideration.", "contents": "Energy requirements for damaging DNA molecules. III. The mechanisms of inactivation of bacteriophage chiX 174 DNA by vacuum ultra-violet radiation. Strand break formation and biological inactivation of infectious DNA of bacteriophage phi chi 174 exposed to vacuum-ultra-violet radiation of 4-9 to 21-2 eV quantum energy is investigated. At 21-2 eV as many as 84 per cent of the DNA molecules are inactivated by breaks whereas breaks do not contribute to inactivation at 4-9 eV. The quantum yield of break formation increases from 1-7 X 10(-5) (4-9 EV) to 0-55 (21-2 eV) and shows a dependence on energy similar to that of electron emission (due to ionization) above 8 eV. The mechanisms leading to break formation and inactivation are discussed taking the absorption spectrum of DNA in the vacuum-ultra-violet region into consideration."}
{"id": "PMID:1081081", "title": "Further studies of DNA damage and lethality from the decay of iodine-125 in bacteriophages.", "content": "The DNA of coliphages T4 and T1 was labelled with 125I-iododeoxyuridine. 125I decay is known to cause severe molecular damage via vacancy cascades (the Auger effect). We have compared the induction of both single- and double-strand breaks (SSBs and DSBs) in 125I-labelled T4 DNA stored at - 196 degrees C during decay, either as intact phage or as free DNA. These comparative experiments indicate that, in addition to one DSB which apparently results directly from the Auger effect, each decay in an intact phage also give rise to an additional 0-05 DSBs, as well as 1-6 SSBs, as a result of ionizing radiation absorbed in the same phage particle where the decay occurs. An examination of T4-killing by 125I decay reveals a two-phase survival curve, whose initial slope corresponds to a lethal efficency per 125I decay of 0-95 +/- 0-05, which is considerably higher than values previously determined. The results for phage T4, and of a more limited comparison of 125I suicide and DNA damage in phage T1, support the hypothesis that the vacancy cascades which accompany each 125I decay in DNA result in a double-strand break at the decay site and that each such break is a lethal event.", "contents": "Further studies of DNA damage and lethality from the decay of iodine-125 in bacteriophages. The DNA of coliphages T4 and T1 was labelled with 125I-iododeoxyuridine. 125I decay is known to cause severe molecular damage via vacancy cascades (the Auger effect). We have compared the induction of both single- and double-strand breaks (SSBs and DSBs) in 125I-labelled T4 DNA stored at - 196 degrees C during decay, either as intact phage or as free DNA. These comparative experiments indicate that, in addition to one DSB which apparently results directly from the Auger effect, each decay in an intact phage also give rise to an additional 0-05 DSBs, as well as 1-6 SSBs, as a result of ionizing radiation absorbed in the same phage particle where the decay occurs. An examination of T4-killing by 125I decay reveals a two-phase survival curve, whose initial slope corresponds to a lethal efficency per 125I decay of 0-95 +/- 0-05, which is considerably higher than values previously determined. The results for phage T4, and of a more limited comparison of 125I suicide and DNA damage in phage T1, support the hypothesis that the vacancy cascades which accompany each 125I decay in DNA result in a double-strand break at the decay site and that each such break is a lethal event."}
{"id": "PMID:1081082", "title": "Radiation dose from plutonium deposited in marrow and bone of normal and chimaeric mice.", "content": "239Pu citrate was injected intraperitoneally into CBA mice and into CBA mice which had been made chimaeric by replacing their haematopoietic bone-marrow with that from another genetically-identical but cytologically-distinct strain. The mice were killed at three intervals up to 90 days after injection, and the deposited 239Pu was determined radiochemically in bone-marrow and in bone. The average radiation dose (integrated over 90 days) was found to be greater in the bones but lower in the marrow of chimaeric mice than in the corresponding tissues of the normal CBA mice. These results are discussed from the points of view of induction of malignant change in marrow and bone and of plutonium metabolism in the two types of mouse.", "contents": "Radiation dose from plutonium deposited in marrow and bone of normal and chimaeric mice. 239Pu citrate was injected intraperitoneally into CBA mice and into CBA mice which had been made chimaeric by replacing their haematopoietic bone-marrow with that from another genetically-identical but cytologically-distinct strain. The mice were killed at three intervals up to 90 days after injection, and the deposited 239Pu was determined radiochemically in bone-marrow and in bone. The average radiation dose (integrated over 90 days) was found to be greater in the bones but lower in the marrow of chimaeric mice than in the corresponding tissues of the normal CBA mice. These results are discussed from the points of view of induction of malignant change in marrow and bone and of plutonium metabolism in the two types of mouse."}
{"id": "PMID:1081083", "title": "Modification of the radiosensitivity of barley seed by post-treatment with caffeine. Kinetics of decay of caffeine-reactive oxygen-sensitive sites.", "content": "In dry barley seeds (3-1 per cent moisture content), the maximal level of oxic damage (class III damage) is reached within the first 40 min of post-hydration in oxygenated water at 5 +/- 1 degrees C. The decay of the gamma-ray-induced oxygen-sensitive sites requires, however, about 120 min. The mechanisms leading to partial protection against the class III damage are initiated when caffeine is present during the first 30 min of oxygenated post-hydration. If added after 30 min of oxygenated hydration, caffeine has no protective action. For the potentiation of an oxygen-independent component of damage, caffeine has to be present during the first 240 min of oxygen-free hydration. These observations involving a physiologically inert system raise questions regarding physico-chemical vis-a-vis biochemical mechanisms of caffeine effect on irradiated systems and these have been briefly discussed.", "contents": "Modification of the radiosensitivity of barley seed by post-treatment with caffeine. Kinetics of decay of caffeine-reactive oxygen-sensitive sites. In dry barley seeds (3-1 per cent moisture content), the maximal level of oxic damage (class III damage) is reached within the first 40 min of post-hydration in oxygenated water at 5 +/- 1 degrees C. The decay of the gamma-ray-induced oxygen-sensitive sites requires, however, about 120 min. The mechanisms leading to partial protection against the class III damage are initiated when caffeine is present during the first 30 min of oxygenated post-hydration. If added after 30 min of oxygenated hydration, caffeine has no protective action. For the potentiation of an oxygen-independent component of damage, caffeine has to be present during the first 240 min of oxygen-free hydration. These observations involving a physiologically inert system raise questions regarding physico-chemical vis-a-vis biochemical mechanisms of caffeine effect on irradiated systems and these have been briefly discussed."}
{"id": "PMID:1081084", "title": "Oxygen--effect on strand breaks and specific end-groups in DNA of irradiated thymocytes.", "content": "Thymocytes were irradiated with fast electrons up to 6 Mrad in the presence and absence of oxygen. The cells were treated before irradiation with a cold shock to prevent any repair rejection during irradiation. The DNA isolated subsequently was analysed for double-strand breaks (dsb), actual single-strand breaks (ssb) and alkali-induced strand breaks (alisb). We observed a linear increase of all types of lesions with dose and an o.e.r. for dsb of 3-6, for ssb of 4-9 and for alisb of 2-1. The data do not deviate significantly from those, measured on thymocytes irradiated without cold shock. In DNA of irradiated thymocytes, the frequency of 3' and 5' hydroxyl and 5' phosphate end-groups was analysed enzymatically. In both the ssb and alisb, about 11 per cent of the terminals carry 5'OH end-groups and 20-40 per cent 5' phosphate groups. On the 3' terminals, 60-80 per cent of the ssb are identified as 3'OH end-groups, whereas on the alisb only a small amount of 3'OH end-groups if found. The frequency of characterized end-groups shows the same oxygen effect as the corresponding strand breaks. Therefore, in the presence and absence of oxygen, the same mechanism may be responsible for formation of DNA strand breaks in vivo.", "contents": "Oxygen--effect on strand breaks and specific end-groups in DNA of irradiated thymocytes. Thymocytes were irradiated with fast electrons up to 6 Mrad in the presence and absence of oxygen. The cells were treated before irradiation with a cold shock to prevent any repair rejection during irradiation. The DNA isolated subsequently was analysed for double-strand breaks (dsb), actual single-strand breaks (ssb) and alkali-induced strand breaks (alisb). We observed a linear increase of all types of lesions with dose and an o.e.r. for dsb of 3-6, for ssb of 4-9 and for alisb of 2-1. The data do not deviate significantly from those, measured on thymocytes irradiated without cold shock. In DNA of irradiated thymocytes, the frequency of 3' and 5' hydroxyl and 5' phosphate end-groups was analysed enzymatically. In both the ssb and alisb, about 11 per cent of the terminals carry 5'OH end-groups and 20-40 per cent 5' phosphate groups. On the 3' terminals, 60-80 per cent of the ssb are identified as 3'OH end-groups, whereas on the alisb only a small amount of 3'OH end-groups if found. The frequency of characterized end-groups shows the same oxygen effect as the corresponding strand breaks. Therefore, in the presence and absence of oxygen, the same mechanism may be responsible for formation of DNA strand breaks in vivo."}
{"id": "PMID:1081087", "title": "Radiosensitivity and hydroxyl radical reactivity of phosphate esters as measured by radiation-induced dephosphorylation.", "content": "The yields of inorganic phosphate from irradiated phosphate esters of biological interest have been measured in the presence of various free-radical scavengers. These studies indicate that hydroxyl radical attack on the phosphate esters accounts for most of this damage. Competition kinetics using different hydroxyl radical scavengers whose reactivities are known have been used to estimate the rate-constants for the overall hydroxyl radical reactivities of the phosphate esters. The extent of hydroxyl-radical-induced dephosphorylation is very low for nucleotide derivatives, but high for some sugar and glycerol phosphates, reflecting the probability of hydroxyl radical abstraction at the alpha or beta carbon atoms adjacent to the phosphate ester linkage. The hydroxyl radical reactivities of nucleotides, coenzymes, sugar phosphates and phospholipid components are all high (1-10 X 10(9) M-1 sec-1), indicating the importance of hydroxyl radical attack in the inactivation of these components in living cells, although not necessarily by dephosphorylation.", "contents": "Radiosensitivity and hydroxyl radical reactivity of phosphate esters as measured by radiation-induced dephosphorylation. The yields of inorganic phosphate from irradiated phosphate esters of biological interest have been measured in the presence of various free-radical scavengers. These studies indicate that hydroxyl radical attack on the phosphate esters accounts for most of this damage. Competition kinetics using different hydroxyl radical scavengers whose reactivities are known have been used to estimate the rate-constants for the overall hydroxyl radical reactivities of the phosphate esters. The extent of hydroxyl-radical-induced dephosphorylation is very low for nucleotide derivatives, but high for some sugar and glycerol phosphates, reflecting the probability of hydroxyl radical abstraction at the alpha or beta carbon atoms adjacent to the phosphate ester linkage. The hydroxyl radical reactivities of nucleotides, coenzymes, sugar phosphates and phospholipid components are all high (1-10 X 10(9) M-1 sec-1), indicating the importance of hydroxyl radical attack in the inactivation of these components in living cells, although not necessarily by dephosphorylation."}
{"id": "PMID:1081088", "title": "Viruses in osteosarcomas induced by 226Ra. A study of the induction of bone tumours in mice.", "content": "Three groups (15 in each) of 3-month-old mice were injected intraperitoneally with 0-5 muCi 226Ra. They were male and female C3H/H and female CBA/H. The groups were matched by similar controls that were not injected. Osteosarcomas were found in 14/15 of the female C3H/H mice--animals known to carry mammary-tumour viruses in milk--compared with 6/15 in their male counterparts. In CBA/H female mice, which have a very low incidence of both mammary tumours and leukaemia, osteo-sarcomas developed in 7/15 of the animals. The CBA/H mice lived about 5-8 months longer and revealed their osteosarcomas at a correspondingly later time than C3H/H mice. Virus particles were observed in each of the three mammary tumours and in 15 out of the 17 osteosarcomas examined by electron microscopy. No definitive statement can be made from this experiment whether or not osteosarcoma was caused by a virus; but the results suggest that viruses may be a contributory factor in the development of osteosarcoma in these animals.", "contents": "Viruses in osteosarcomas induced by 226Ra. A study of the induction of bone tumours in mice. Three groups (15 in each) of 3-month-old mice were injected intraperitoneally with 0-5 muCi 226Ra. They were male and female C3H/H and female CBA/H. The groups were matched by similar controls that were not injected. Osteosarcomas were found in 14/15 of the female C3H/H mice--animals known to carry mammary-tumour viruses in milk--compared with 6/15 in their male counterparts. In CBA/H female mice, which have a very low incidence of both mammary tumours and leukaemia, osteo-sarcomas developed in 7/15 of the animals. The CBA/H mice lived about 5-8 months longer and revealed their osteosarcomas at a correspondingly later time than C3H/H mice. Virus particles were observed in each of the three mammary tumours and in 15 out of the 17 osteosarcomas examined by electron microscopy. No definitive statement can be made from this experiment whether or not osteosarcoma was caused by a virus; but the results suggest that viruses may be a contributory factor in the development of osteosarcoma in these animals."}
{"id": "PMID:1081089", "title": "Radioprotection of lipogenesis from glucose (U-14C) and activities of pyridine nucleotide dehydrogenases in liver of gamma-irradiated female rats by cystamine.", "content": "Whole-body gamma-irradiation (1200 R) of female rats significantly increased liver glycogen and incorporation of glucose (U-14C) into liver fatty acids. Administration of cystamine before irradiation protected the enhanced lipogenesis from glucose (U-14C), but afforded only a partial protection of liver glycogen levels. Irradiation significantly decreased the activities of liver pyruvate kinase and isocitrate dehydrogenase, and this decrease was not prevented by the administration of cystamine before irradiation. A significant increase in the activity of liver malate dehydrogenase (dehydrogenating) was noted in irradiated rats, which was not prevented by the administration of cystamine before irradiation. Irradiation did not affect the activities of combined hexose monophosphate pathway dehydrogenases and of malate dehydrogenase (decarboxylating).", "contents": "Radioprotection of lipogenesis from glucose (U-14C) and activities of pyridine nucleotide dehydrogenases in liver of gamma-irradiated female rats by cystamine. Whole-body gamma-irradiation (1200 R) of female rats significantly increased liver glycogen and incorporation of glucose (U-14C) into liver fatty acids. Administration of cystamine before irradiation protected the enhanced lipogenesis from glucose (U-14C), but afforded only a partial protection of liver glycogen levels. Irradiation significantly decreased the activities of liver pyruvate kinase and isocitrate dehydrogenase, and this decrease was not prevented by the administration of cystamine before irradiation. A significant increase in the activity of liver malate dehydrogenase (dehydrogenating) was noted in irradiated rats, which was not prevented by the administration of cystamine before irradiation. Irradiation did not affect the activities of combined hexose monophosphate pathway dehydrogenases and of malate dehydrogenase (decarboxylating)."}
{"id": "PMID:1081090", "title": "Influence of rat blood on radiation protection of mammalian cells by cysteamine and cystamine in vitro.", "content": "The protective effect of cystamine and cystamine on T-cells in normal growth-medium was studied in the presence of whole rate blood (WRB). The protective effectiveness of both agents was increased by the addition of WRB, but much less so on the activity of cystamine. In the presence of WRB a dose-reduction factor of 1-5 was obtained at the low concentration of 0-05 mM cysteamine. The increase in protection was not due to induction of anoxia or to a release of glutathione from the rat red cell. The increase in protection may be due to protector RS radicals reacting with rat red cells.", "contents": "Influence of rat blood on radiation protection of mammalian cells by cysteamine and cystamine in vitro. The protective effect of cystamine and cystamine on T-cells in normal growth-medium was studied in the presence of whole rate blood (WRB). The protective effectiveness of both agents was increased by the addition of WRB, but much less so on the activity of cystamine. In the presence of WRB a dose-reduction factor of 1-5 was obtained at the low concentration of 0-05 mM cysteamine. The increase in protection was not due to induction of anoxia or to a release of glutathione from the rat red cell. The increase in protection may be due to protector RS radicals reacting with rat red cells."}
{"id": "PMID:1081091", "title": "Quantitative dose-response of growth and development in Arabidopsis thaliana exposed to chronic gamma-radiation.", "content": "The response of Arabidopsis thaliana (L.) Heynh. (Cruciferae) to a gradient of chronic gamma-radiation was examined under field conditions. Plants that were initially introduced to the gamma field as dry seeds received exposures of 1-34 to 18 800 R/20 hour day from the time of seed germination. Regression analysis demonstrated a significant, but non-linear, response for three variables, number of seedlings emerging, number of plants flowering, and plant volume; the response of a fourth variable, number of leaves per plant, was not related to daily exposure. LD50 values ranged from 66 R/20 hour day for plant volume to 1231 R/20 hour day for seedling emergence. Flowering and plant volume were the most sensitive indicators of radiation exposure. The demonstration of a variable threshold at low levels of exposure indicates that in nature A. thaliana may be exposed to environmental radiation throughout its life-cycle without significant modification of growth or development.", "contents": "Quantitative dose-response of growth and development in Arabidopsis thaliana exposed to chronic gamma-radiation. The response of Arabidopsis thaliana (L.) Heynh. (Cruciferae) to a gradient of chronic gamma-radiation was examined under field conditions. Plants that were initially introduced to the gamma field as dry seeds received exposures of 1-34 to 18 800 R/20 hour day from the time of seed germination. Regression analysis demonstrated a significant, but non-linear, response for three variables, number of seedlings emerging, number of plants flowering, and plant volume; the response of a fourth variable, number of leaves per plant, was not related to daily exposure. LD50 values ranged from 66 R/20 hour day for plant volume to 1231 R/20 hour day for seedling emergence. Flowering and plant volume were the most sensitive indicators of radiation exposure. The demonstration of a variable threshold at low levels of exposure indicates that in nature A. thaliana may be exposed to environmental radiation throughout its life-cycle without significant modification of growth or development."}
{"id": "PMID:1081092", "title": "The relationship between chromosome aberrations and low LET radiation dose to human lymphocytes.", "content": "In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60gamma-radiation. The aberration yields have been fitted to the quadratic function Y = alphaD +betaD2, which is consistent with the single-track and two-track model for aberration formation. The values of the coefficients alpha and beta support the hypothesis that the dose-rate effect is limited to the D2 term. The main difference between the coefficients for X- and gamma-radiation is in the alpha values, indicating that X-rays are slightly more efficient, at lower doses, in producing two lesions with a single ionizing track. The lower limits of dose estimate, with 500 cells analysed, are 4 rad for X-rays and 10 rad gamma-radiation. Further evidence is presented confirming that, for cytogenetic dosimetry, in vitro dose-response curves should be prepared by irradiating whole blood maintained at 37 degrees C and prior to PHA stimulation. Curves were plotted showing the variation of the number of cells without aberrations with radiation dose and the shape of these curves were compared with those from human cell survival experiments.", "contents": "The relationship between chromosome aberrations and low LET radiation dose to human lymphocytes. In vitro dose-response curves of unstable chromosome aberrations in human lymphocytes have been obtained for 250 kV X-rays and cobalt-60gamma-radiation. The aberration yields have been fitted to the quadratic function Y = alphaD +betaD2, which is consistent with the single-track and two-track model for aberration formation. The values of the coefficients alpha and beta support the hypothesis that the dose-rate effect is limited to the D2 term. The main difference between the coefficients for X- and gamma-radiation is in the alpha values, indicating that X-rays are slightly more efficient, at lower doses, in producing two lesions with a single ionizing track. The lower limits of dose estimate, with 500 cells analysed, are 4 rad for X-rays and 10 rad gamma-radiation. Further evidence is presented confirming that, for cytogenetic dosimetry, in vitro dose-response curves should be prepared by irradiating whole blood maintained at 37 degrees C and prior to PHA stimulation. Curves were plotted showing the variation of the number of cells without aberrations with radiation dose and the shape of these curves were compared with those from human cell survival experiments."}
{"id": "PMID:1081095", "title": "The electroretinogram in dogs with inherited cone degeneration.", "content": "The electroretinogram (ERG) of hemeralopic Alaskan malamute dogs contains only rod components. There is absence of the photopic b-wave which is normally elicited with red light stimuli during dark adaptation and, using flicker stimulation, only the first or rod branch of the flicker fusion response curve is present. At high stimulus intensity levels, the flicker response of hemeralopes is absent. A normal ERG is recorded from affected dogs using blue light stimuli and low intensity white light. In the adult hemeralope, the retina contains no cones.", "contents": "The electroretinogram in dogs with inherited cone degeneration. The electroretinogram (ERG) of hemeralopic Alaskan malamute dogs contains only rod components. There is absence of the photopic b-wave which is normally elicited with red light stimuli during dark adaptation and, using flicker stimulation, only the first or rod branch of the flicker fusion response curve is present. At high stimulus intensity levels, the flicker response of hemeralopes is absent. A normal ERG is recorded from affected dogs using blue light stimuli and low intensity white light. In the adult hemeralope, the retina contains no cones."}
{"id": "PMID:1081099", "title": "Anti-nucleic acid antibodies in systemic lupus erythematosus patients and their families. Incidence and correlation with lymphocytotoxic antibodies.", "content": "Anti-RNA antibodies were found in 82% of 28 systemic lupus erythematosus (SLE) probands and in 16% of 124 of their family members. The incidence in 76 control family members was only 5%. In the SLE family members, the antibodies were found exclusively in 21% of the 94 close household contacts of the probands. The incidence of anti-native DNA (nDNA) antibodies was 68% for the SLE probands. The incidence of anti-nDNA antibodies in close household contacts of the probands was 6%, which was not significantly different from the 1% incidence found in control families. Lymphocytotoxic antibodies occurred in 57% of the SLE family members as a whole and in 68% of the close household contacts. In the SLE probands, lymphocytotoxic antibodies correlated with anti-single-stranded RNA (poly A) and anti-nDNA but not with anti-double-stranded RNA (poly A-poly U). On the other hand, lymphocytotoxic antibodies in the household contacts correlated with anti-double-stranded RNA (poly A-poly U) but not with anti-poly A or anti-nDNA. The anti-RNA antibodies were present in consanguineous household contacts but not in nonconsanguineous household contacts. These findings strengthen the hypothesis that both an environmental agent, possibly a virus, as well as the genetic response are important in the pathogenesis of SLE. Family members may therefore be a logical population in whom to search for specific antibodies to a viral agent.", "contents": "Anti-nucleic acid antibodies in systemic lupus erythematosus patients and their families. Incidence and correlation with lymphocytotoxic antibodies. Anti-RNA antibodies were found in 82% of 28 systemic lupus erythematosus (SLE) probands and in 16% of 124 of their family members. The incidence in 76 control family members was only 5%. In the SLE family members, the antibodies were found exclusively in 21% of the 94 close household contacts of the probands. The incidence of anti-native DNA (nDNA) antibodies was 68% for the SLE probands. The incidence of anti-nDNA antibodies in close household contacts of the probands was 6%, which was not significantly different from the 1% incidence found in control families. Lymphocytotoxic antibodies occurred in 57% of the SLE family members as a whole and in 68% of the close household contacts. In the SLE probands, lymphocytotoxic antibodies correlated with anti-single-stranded RNA (poly A) and anti-nDNA but not with anti-double-stranded RNA (poly A-poly U). On the other hand, lymphocytotoxic antibodies in the household contacts correlated with anti-double-stranded RNA (poly A-poly U) but not with anti-poly A or anti-nDNA. The anti-RNA antibodies were present in consanguineous household contacts but not in nonconsanguineous household contacts. These findings strengthen the hypothesis that both an environmental agent, possibly a virus, as well as the genetic response are important in the pathogenesis of SLE. Family members may therefore be a logical population in whom to search for specific antibodies to a viral agent."}
{"id": "PMID:1081100", "title": "Adult alpha1-antitrypsin deficiency.", "content": "Three adults with alpha 1-antitrypsin deficiency are described. In two of the cases the deficiency was genetically determined (cases 1 and 2), and each demonstrated unusual features of the disease. The liver in case 1 (homozygous) showed cholangiolar hyperplasia which has been recorded only once before. Case 2 (heterozygous) had emphysema and cirrhosis, a combination not previously documented in a heterozygote, in addition to malabsorption. Case 3 represents a case of spurious alpha 1-antitrypsin deficiency with cirrhosis included to emphasize the diagnostic improtance of phenotyping in such cases.", "contents": "Adult alpha1-antitrypsin deficiency. Three adults with alpha 1-antitrypsin deficiency are described. In two of the cases the deficiency was genetically determined (cases 1 and 2), and each demonstrated unusual features of the disease. The liver in case 1 (homozygous) showed cholangiolar hyperplasia which has been recorded only once before. Case 2 (heterozygous) had emphysema and cirrhosis, a combination not previously documented in a heterozygote, in addition to malabsorption. Case 3 represents a case of spurious alpha 1-antitrypsin deficiency with cirrhosis included to emphasize the diagnostic improtance of phenotyping in such cases."}
{"id": "PMID:1081102", "title": "Heterogeneity of human T lymphocytes to bind sheep erythrocytes and mitogenic responses of their subpopulations.", "content": "Heterogeneity of human T lymphocytes was examined by the rosette formation of sheep red blood cells (E-rosette) in two different reaction media: fetal calf serum (FCS) and gelatin Veronal buffer (GVB). Nearly all the T lymphocytes could form rosettes in FCS at 1 degrees C (FCS-R) independently of the incubation time. In contrast, rosette formation in GVB (GVB-R) was apparently dependent upon the incubation time and could not be used in the enumeration of the total T cells even after long incubation. Among the possible subpopulations, a fraction (T1) that could form GVB-R as well as FCS-R, and the other fraction (T2) that could form only FCS-R were partially purified by rosette formation and by density gradient on a Ficoll-Isopaque layer. In contrast to the T cell-depleted fraction (B) which formed EAC rosettes in a majority of cells and E rosettes in a few cells, only a small portion of cells from both the T1 and T2 fractions could form EAC-rosettes. T1 cells possessed high responses to PHA as estimated by the counts of 3H-TdR incorporation as well as the stimulation indices. In contrast, T2 cells possessed a lower responsiveness to the mitogen, and B fraction cells could not respond at all. These results suggested that a positive correlation exists between the ability of E rosette formation and responsiveness to PHA in the T cell subpopulations. Preliminary experiments also suggested that percentages of T1 cells highly responsive to PHA may decrease and, therefore, those of T2 cells less responsive to the mitogen may increase in the T cell population of the patients with some immunodeficiencies.", "contents": "Heterogeneity of human T lymphocytes to bind sheep erythrocytes and mitogenic responses of their subpopulations. Heterogeneity of human T lymphocytes was examined by the rosette formation of sheep red blood cells (E-rosette) in two different reaction media: fetal calf serum (FCS) and gelatin Veronal buffer (GVB). Nearly all the T lymphocytes could form rosettes in FCS at 1 degrees C (FCS-R) independently of the incubation time. In contrast, rosette formation in GVB (GVB-R) was apparently dependent upon the incubation time and could not be used in the enumeration of the total T cells even after long incubation. Among the possible subpopulations, a fraction (T1) that could form GVB-R as well as FCS-R, and the other fraction (T2) that could form only FCS-R were partially purified by rosette formation and by density gradient on a Ficoll-Isopaque layer. In contrast to the T cell-depleted fraction (B) which formed EAC rosettes in a majority of cells and E rosettes in a few cells, only a small portion of cells from both the T1 and T2 fractions could form EAC-rosettes. T1 cells possessed high responses to PHA as estimated by the counts of 3H-TdR incorporation as well as the stimulation indices. In contrast, T2 cells possessed a lower responsiveness to the mitogen, and B fraction cells could not respond at all. These results suggested that a positive correlation exists between the ability of E rosette formation and responsiveness to PHA in the T cell subpopulations. Preliminary experiments also suggested that percentages of T1 cells highly responsive to PHA may decrease and, therefore, those of T2 cells less responsive to the mitogen may increase in the T cell population of the patients with some immunodeficiencies."}
{"id": "PMID:1081103", "title": "Genetic control of the murine cell-mediated immune response in vivo. II. H-2 linked responsiveness to the synthetic polypeptide poly(Tyr,Glu)-poly(DL-Ala)--poly(Lys).", "content": "Cell-mediated immunity in response to (T,G)-A-L was studied with a radioisotopic footpad assay. It appears that in vivo cell-mediated immune responsiveness to (T,G)-A--L is linked to the H-2 complex, as was demonstrated previously for specific antibody snythesis. Evidence is presented in this report which indicates that the cell-mediated immunity measured is a function of a population of T cells. The apparent controversy between results obtained by analyzing antibody formation and cell-mediated immunity can be explained by assuming the need for cell cooperation between helper T cell and effector T cell, because efficient purely cellular immunity is analogous to T-B cell cooperation needed for antibody production.", "contents": "Genetic control of the murine cell-mediated immune response in vivo. II. H-2 linked responsiveness to the synthetic polypeptide poly(Tyr,Glu)-poly(DL-Ala)--poly(Lys). Cell-mediated immunity in response to (T,G)-A-L was studied with a radioisotopic footpad assay. It appears that in vivo cell-mediated immune responsiveness to (T,G)-A--L is linked to the H-2 complex, as was demonstrated previously for specific antibody snythesis. Evidence is presented in this report which indicates that the cell-mediated immunity measured is a function of a population of T cells. The apparent controversy between results obtained by analyzing antibody formation and cell-mediated immunity can be explained by assuming the need for cell cooperation between helper T cell and effector T cell, because efficient purely cellular immunity is analogous to T-B cell cooperation needed for antibody production."}
{"id": "PMID:1081101", "title": "Inhibitory effect of Apernyl on production of bradykininlike substance in pulp.", "content": "In the Formalin-induced pulpitis of rats and the electrically stimulated pulps of dogs, a bradykininlike substance developed by the pulps and released into a saline pool on the exposed pulps disappeared after a local application of Apernyl solution. This suggests that the possible pain-reducing or anti-in-flammatory effect of Apernyl is a result of its inhibiting bradykinin production.", "contents": "Inhibitory effect of Apernyl on production of bradykininlike substance in pulp. In the Formalin-induced pulpitis of rats and the electrically stimulated pulps of dogs, a bradykininlike substance developed by the pulps and released into a saline pool on the exposed pulps disappeared after a local application of Apernyl solution. This suggests that the possible pain-reducing or anti-in-flammatory effect of Apernyl is a result of its inhibiting bradykinin production."}
{"id": "PMID:1081104", "title": "Lack of inverse relationship between the net charge of T-independent immunogens and the responding spleen cells.", "content": "Previous studies have demonstrated an inverse relationship between the net electrical charge of immunogens and the antibodies they elicit. This correlation was found to be expressed at the cellular level. It has been shown that thymus-derived cells may recognize immunogens on the basis of their overall electrical charge. In this study, charged T-independent copolymers composed of tyrosine, glutamic acid, and lysine of the D configuration were prepared in order to find out whether this net charge phenomenon holds also for immunogens which do not require helper T cells for generation of immune response. Spleen cells were fractionated over negatively charged glass bead columns, and their immunocompetence was tested by transferring them into irradiated recipient mice which were immunized with the dinitrophenylated acidic or basic T-independent carrier. No differences in the responsiveness to the dinitrophenyl group on either carrier could be detected in recipients of unfractionated or fractionated cells on the charged columns. Similar results were obtained with the negatively charged T-independent branched polypeptide poly-(DTyr,DGlu)-poly(DPro)--poly(DLys). Filtration of spleen cells through glass bead columns did not affect the immune response potential of the recipient mice. In contrast, a significant reduction was observed in the frequency of positive responses in recipients of filtered cells, which were immunized with the negatively charged T-dependent poly(DTyr,DGlu)-poly(DPro)--poly(DLys). Thus, the inverse net charge phenomenon holds only for T-dependent antigens.", "contents": "Lack of inverse relationship between the net charge of T-independent immunogens and the responding spleen cells. Previous studies have demonstrated an inverse relationship between the net electrical charge of immunogens and the antibodies they elicit. This correlation was found to be expressed at the cellular level. It has been shown that thymus-derived cells may recognize immunogens on the basis of their overall electrical charge. In this study, charged T-independent copolymers composed of tyrosine, glutamic acid, and lysine of the D configuration were prepared in order to find out whether this net charge phenomenon holds also for immunogens which do not require helper T cells for generation of immune response. Spleen cells were fractionated over negatively charged glass bead columns, and their immunocompetence was tested by transferring them into irradiated recipient mice which were immunized with the dinitrophenylated acidic or basic T-independent carrier. No differences in the responsiveness to the dinitrophenyl group on either carrier could be detected in recipients of unfractionated or fractionated cells on the charged columns. Similar results were obtained with the negatively charged T-independent branched polypeptide poly-(DTyr,DGlu)-poly(DPro)--poly(DLys). Filtration of spleen cells through glass bead columns did not affect the immune response potential of the recipient mice. In contrast, a significant reduction was observed in the frequency of positive responses in recipients of filtered cells, which were immunized with the negatively charged T-dependent poly(DTyr,DGlu)-poly(DPro)--poly(DLys). Thus, the inverse net charge phenomenon holds only for T-dependent antigens."}
{"id": "PMID:1081105", "title": "Effects of anti-Ia serum on mitogenic responses. I. Inhibition of the proliferative response to B cell mitogen, LPS, by specific anti-Ia sera.", "content": "Specific anti-Ia serum in combination with rabbit complement removed the B lymphocyte population normally responsive to LPS mitogenic stimulation. Significant inhibition of this proliferative response was also obtained by brief periods of cell pretreatment with anti-Ia antibodies, but not with specific anti-H-2K antibodies. The population of splenic B cells resistant to anti-Thy-1.2 serum and complement was blocked in a very similar manner by anti-Ia serum, but not by anti-H-2 serum.", "contents": "Effects of anti-Ia serum on mitogenic responses. I. Inhibition of the proliferative response to B cell mitogen, LPS, by specific anti-Ia sera. Specific anti-Ia serum in combination with rabbit complement removed the B lymphocyte population normally responsive to LPS mitogenic stimulation. Significant inhibition of this proliferative response was also obtained by brief periods of cell pretreatment with anti-Ia antibodies, but not with specific anti-H-2K antibodies. The population of splenic B cells resistant to anti-Thy-1.2 serum and complement was blocked in a very similar manner by anti-Ia serum, but not by anti-H-2 serum."}
{"id": "PMID:1081106", "title": "Permanent slide preparations of T lymphocyte-sheep red blood cell rosettes.", "content": "Rosette formation between sheep erythrocytes (SRBC) and human thymus-derived lymphocytes (T cells) is used to monitor T cells in various human diseases. Rosettes are usually counted in a hemacytometer immediately after preparation. This paper reports a technique for permanently fixing and staining rosettes for serial and comparative studies, a procedure which has probably been less well standardized, less reproducibly performed, and less widely used than many investigators appreciate. The technique describedhas the advantages of providing distinct morphological identification of the rosette-forming cell and it produces a permanent mount for further reference.", "contents": "Permanent slide preparations of T lymphocyte-sheep red blood cell rosettes. Rosette formation between sheep erythrocytes (SRBC) and human thymus-derived lymphocytes (T cells) is used to monitor T cells in various human diseases. Rosettes are usually counted in a hemacytometer immediately after preparation. This paper reports a technique for permanently fixing and staining rosettes for serial and comparative studies, a procedure which has probably been less well standardized, less reproducibly performed, and less widely used than many investigators appreciate. The technique describedhas the advantages of providing distinct morphological identification of the rosette-forming cell and it produces a permanent mount for further reference."}
{"id": "PMID:1081119", "title": "Aspects of tail muscle ultrastructure and its degeneration in Rana temporaria.", "content": "1. Tail muscles of larval Rana temporaria and to a lesser extent Xenopus laevis, at prometamorphosis, were investigated by light and electron microscopy. In the case of Rana their degeneration is described at metamorphic climax. 2. The non-degenerate tail muscles of both genera are generally similar in their ultrastructure and likewise similar to those striated muscles of other adult vertebrates including amphibians. 3. At climax tail muscles of Rana degenerate by autolysis. It seems likely that lysosomal enzymes are directly involved in sarcoplasmic degeneration. The relationship between lysosomes and myofibrillar degradation is difficult to establish. The subject is discussed in the light of relevant information (mainly mammalian) on muscle dystrophies. 4. Anuran tail muscle is highly degraded before its probable phagocytosis by mesenchymal macrophages.", "contents": "Aspects of tail muscle ultrastructure and its degeneration in Rana temporaria. 1. Tail muscles of larval Rana temporaria and to a lesser extent Xenopus laevis, at prometamorphosis, were investigated by light and electron microscopy. In the case of Rana their degeneration is described at metamorphic climax. 2. The non-degenerate tail muscles of both genera are generally similar in their ultrastructure and likewise similar to those striated muscles of other adult vertebrates including amphibians. 3. At climax tail muscles of Rana degenerate by autolysis. It seems likely that lysosomal enzymes are directly involved in sarcoplasmic degeneration. The relationship between lysosomes and myofibrillar degradation is difficult to establish. The subject is discussed in the light of relevant information (mainly mammalian) on muscle dystrophies. 4. Anuran tail muscle is highly degraded before its probable phagocytosis by mesenchymal macrophages."}
{"id": "PMID:1081120", "title": "Visual reaction times during prolonged angular acceleration parallel the subjective perception of rotation.", "content": "The effect of prolonged angular acceleration on choice reaction time to an accelerating visual stimulus was investigated, with 10 commercial airline pilots serving as subjects. The pattern of reaction times during and following acceleration was compared with the pattern of velocity estimates reported during identical trials. Both reaction times and velocity estimates increased at the onset of acceleration, declined prior to the termination of acceleration, and showed an aftereffect. These results are inconsistent with the torsion-pendulum theory of semicircular canal function and suggest that the vestibular adaptation is of central origin.", "contents": "Visual reaction times during prolonged angular acceleration parallel the subjective perception of rotation. The effect of prolonged angular acceleration on choice reaction time to an accelerating visual stimulus was investigated, with 10 commercial airline pilots serving as subjects. The pattern of reaction times during and following acceleration was compared with the pattern of velocity estimates reported during identical trials. Both reaction times and velocity estimates increased at the onset of acceleration, declined prior to the termination of acceleration, and showed an aftereffect. These results are inconsistent with the torsion-pendulum theory of semicircular canal function and suggest that the vestibular adaptation is of central origin."}
{"id": "PMID:1081121", "title": "Problem-specific coding systems.", "content": "The data found in medical records, particularly in problem-oriented records, could yield very useful information for many aspects of the health care delivery system. Unless the tools and methods used in the analysis of health care data are very carefully designed, the potential value of this information cannot be fully realized. Coding is a critical step in the process of converting medical record data into useful information. This paper presents six criteria as design goals for a standard, comprehensive, coherent set of nomenclatures, classifications, taxonomies, and corresponding coding schemes for the analysis of medical and administrative record data. These goals are: compatibility with established routine operating procedure, health care problem specificity, hierarchical structure, economy through articulation of the components with one another and through the similarities in various aspects of health care delivery, and adaptability to change.", "contents": "Problem-specific coding systems. The data found in medical records, particularly in problem-oriented records, could yield very useful information for many aspects of the health care delivery system. Unless the tools and methods used in the analysis of health care data are very carefully designed, the potential value of this information cannot be fully realized. Coding is a critical step in the process of converting medical record data into useful information. This paper presents six criteria as design goals for a standard, comprehensive, coherent set of nomenclatures, classifications, taxonomies, and corresponding coding schemes for the analysis of medical and administrative record data. These goals are: compatibility with established routine operating procedure, health care problem specificity, hierarchical structure, economy through articulation of the components with one another and through the similarities in various aspects of health care delivery, and adaptability to change."}
{"id": "PMID:1081122", "title": "Characterization of the effects of Mg2+ on Ca2+- and Sr2+-activated tension generation of skinned skeletal muscle fibers.", "content": "Changes in [Mg2+] in a millimolar range have a significant inverse effect on the Ca2+- (or Sr2+)activated tension generation of skeletal muscle fibers. Single frog (Rana pipiens) semitendinosus muscle fibers were \"skinned\" (sarcolemma removed) and contracted isometrically in bathing solutions of varying [Ca2+] or [Sr2+] and [Mg2+] but a constant pH, [MgATP2-], [K+], [CP2-], [CPK], and ionic strength. Ca2+- (or Sr2+-)activated steady-state tensions were recorded for three [Mg2+]'s: 5 X 10(-5)M, 1 X 10(-3) M, and 2 X 10(-3) M; and these tensions were expressed as the percentages of maximum tension generation of the fibers for the same [Mg2+]. Maximum tension was not affected by [Mg2+] within Ca2+-activating or Sr2+-activating sets of solutions; however, the submaximum Ca2+-(or Sr2+)activated tension is strongly affected in an inverse fashion by increasing [Mg2+]. Mg2+ behaves as a competitive inhibitor of Ca2+ and also affects the degree of cooperativity in the system. At [Mg2+] = 5 X 10(-5)M the shape of tension versus [Ca2+] (or [Sr2+]) curve showed evidence of cooperativity of Ca2+ (or Sr2+) binding or activation of the contractile system. As [Mg2+] increased, the apparent affinity for Ca2+ or Sr2+ and cooperativity of the contractile system declined. The effect on cooperativity suggests that as [Mg2+] decreases a threshold for Ca2+ activation appears.", "contents": "Characterization of the effects of Mg2+ on Ca2+- and Sr2+-activated tension generation of skinned skeletal muscle fibers. Changes in [Mg2+] in a millimolar range have a significant inverse effect on the Ca2+- (or Sr2+)activated tension generation of skeletal muscle fibers. Single frog (Rana pipiens) semitendinosus muscle fibers were \"skinned\" (sarcolemma removed) and contracted isometrically in bathing solutions of varying [Ca2+] or [Sr2+] and [Mg2+] but a constant pH, [MgATP2-], [K+], [CP2-], [CPK], and ionic strength. Ca2+- (or Sr2+-)activated steady-state tensions were recorded for three [Mg2+]'s: 5 X 10(-5)M, 1 X 10(-3) M, and 2 X 10(-3) M; and these tensions were expressed as the percentages of maximum tension generation of the fibers for the same [Mg2+]. Maximum tension was not affected by [Mg2+] within Ca2+-activating or Sr2+-activating sets of solutions; however, the submaximum Ca2+-(or Sr2+)activated tension is strongly affected in an inverse fashion by increasing [Mg2+]. Mg2+ behaves as a competitive inhibitor of Ca2+ and also affects the degree of cooperativity in the system. At [Mg2+] = 5 X 10(-5)M the shape of tension versus [Ca2+] (or [Sr2+]) curve showed evidence of cooperativity of Ca2+ (or Sr2+) binding or activation of the contractile system. As [Mg2+] increased, the apparent affinity for Ca2+ or Sr2+ and cooperativity of the contractile system declined. The effect on cooperativity suggests that as [Mg2+] decreases a threshold for Ca2+ activation appears."}
{"id": "PMID:1081126", "title": "Meningiomas of Meckel's cave.", "content": "A retrospective review of 12 cases of meningioma of Meckel's cave involving the Gasserian ganglion or the trigeminal posterior root (or both) seen at the Mayo Clinic during a 20-year period suggested three clearly defined clinical groups. One group (the largest) had typical trigeminal neuralgia and an excellent prognosis after the removal of the easily detachable mass that was impinging on the ganglion.  A second group, with meningiomas en plaque embedded in the ganglion, had a history of atypical trigeminal face pain without neurological deficit, but the prognosis for pain relief was not as good as in the previous group. A third group had a history of face dysesthesias and pain, objective trigeminal sensory loss, and multiple cranial nerve deficit; these patients had meningiomas with histological signs of mitotic activity and a poor prognosis, with return of intractable pain and recurrence of the tumor.", "contents": "Meningiomas of Meckel's cave. A retrospective review of 12 cases of meningioma of Meckel's cave involving the Gasserian ganglion or the trigeminal posterior root (or both) seen at the Mayo Clinic during a 20-year period suggested three clearly defined clinical groups. One group (the largest) had typical trigeminal neuralgia and an excellent prognosis after the removal of the easily detachable mass that was impinging on the ganglion.  A second group, with meningiomas en plaque embedded in the ganglion, had a history of atypical trigeminal face pain without neurological deficit, but the prognosis for pain relief was not as good as in the previous group. A third group had a history of face dysesthesias and pain, objective trigeminal sensory loss, and multiple cranial nerve deficit; these patients had meningiomas with histological signs of mitotic activity and a poor prognosis, with return of intractable pain and recurrence of the tumor."}
{"id": "PMID:1081127", "title": "Rhabdomyosarcoma of the brain.", "content": "The authors report a case of rhabdomyosarcoma originating in the fourth ventricle and review the eight comparable previous reports on true rhabdomyosarcoma, composed solely of mesenchymal elements. Tumors in most adults arose in the cerebral hemisphere, while those in children arose exclusively in the midline structures of the posterior fossa. The tumor in the author's case was initially benign and well circumscribed, but within 2 years had changed into a malignant rhabdomyosarcoma. The histological documentation during the interval is presented and discussed.", "contents": "Rhabdomyosarcoma of the brain. The authors report a case of rhabdomyosarcoma originating in the fourth ventricle and review the eight comparable previous reports on true rhabdomyosarcoma, composed solely of mesenchymal elements. Tumors in most adults arose in the cerebral hemisphere, while those in children arose exclusively in the midline structures of the posterior fossa. The tumor in the author's case was initially benign and well circumscribed, but within 2 years had changed into a malignant rhabdomyosarcoma. The histological documentation during the interval is presented and discussed."}
{"id": "PMID:1081128", "title": "Ascites from a ventriculoperitoneal shunt.", "content": "An unusual case of ascites secondary to a ventriculoperitoneal shunt is presented. The ascites did not redevelop following diversion of the distal catheter into the right atrium, and no adequate explantation was found as to why the cerebrospinal fluid was not absorbed by the peritoneum.", "contents": "Ascites from a ventriculoperitoneal shunt. An unusual case of ascites secondary to a ventriculoperitoneal shunt is presented. The ascites did not redevelop following diversion of the distal catheter into the right atrium, and no adequate explantation was found as to why the cerebrospinal fluid was not absorbed by the peritoneum."}
{"id": "PMID:1081129", "title": "Recognition of traffic signals viewed through colored filters.", "content": "Experiments were carried out to provide an empirical basis for setting color standards for sunglasses. Nineteen individuals, ten with anomalous color vision, viewed traffic signals through a series of color filters. Limits on the apparent color shift for the amber and green signals as well as for D65 are presented on a 1931 CIE chromaticity diagram. Limits on the reduction in the apparent luminosity for each of the three signals are given in terms of the minimum transmittance of the lens for each signal.", "contents": "Recognition of traffic signals viewed through colored filters. Experiments were carried out to provide an empirical basis for setting color standards for sunglasses. Nineteen individuals, ten with anomalous color vision, viewed traffic signals through a series of color filters. Limits on the apparent color shift for the amber and green signals as well as for D65 are presented on a 1931 CIE chromaticity diagram. Limits on the reduction in the apparent luminosity for each of the three signals are given in terms of the minimum transmittance of the lens for each signal."}
{"id": "PMID:1081131", "title": "Central cavernous hemangioma of the mandible treated by an intraoral approach.", "content": "We have presented a case of surgical treatment of a central cavernous hemangioma of the mandible in which a radical deforming operation on a 12-year-old girl was avoided. A rather unique surgical approach was devised after all diagnostic criteria were available and after very careful consideration of an operative plan.", "contents": "Central cavernous hemangioma of the mandible treated by an intraoral approach. We have presented a case of surgical treatment of a central cavernous hemangioma of the mandible in which a radical deforming operation on a 12-year-old girl was avoided. A rather unique surgical approach was devised after all diagnostic criteria were available and after very careful consideration of an operative plan."}
{"id": "PMID:1081133", "title": "Chronic neutropenia: Response to plasma with high colony-stimulating activity.", "content": "A child with repeated infections was immunologically normal but was found to have neutropenia with periodic elevations of the absolute mature polymorphonuclear count at 21-day intervals. Immediately following the PMN rise, bone marrow morphology and in vitro cultures demonstrated a maturation arrest at the myelocyte stage with an increase in proliferative capacity. His cycle was not altered by infusions of normal plasma or by injections of epinephrine or typhoid vaccine. Infusion of 10 ml/kg of \"stimulated\" plasma from donors reactive to TV, obtained 60 minutes following immunization, resulted in an out-of-phase rise in PMN cells and clinical improvement. In vitro assays, using normal or patient marrow, detected high levels of colony-stimulating activity only in those plasma samples that were effective in the patient. These observations support a role of CSA as a physiologic regulator of granulopoiesis in man.", "contents": "Chronic neutropenia: Response to plasma with high colony-stimulating activity. A child with repeated infections was immunologically normal but was found to have neutropenia with periodic elevations of the absolute mature polymorphonuclear count at 21-day intervals. Immediately following the PMN rise, bone marrow morphology and in vitro cultures demonstrated a maturation arrest at the myelocyte stage with an increase in proliferative capacity. His cycle was not altered by infusions of normal plasma or by injections of epinephrine or typhoid vaccine. Infusion of 10 ml/kg of \"stimulated\" plasma from donors reactive to TV, obtained 60 minutes following immunization, resulted in an out-of-phase rise in PMN cells and clinical improvement. In vitro assays, using normal or patient marrow, detected high levels of colony-stimulating activity only in those plasma samples that were effective in the patient. These observations support a role of CSA as a physiologic regulator of granulopoiesis in man."}
{"id": "PMID:1081136", "title": "Blood pressure and puberty.", "content": "The purpose of this investigation was to study the relation of blood pressure to sexual maturation in 229 boys and 189 girls, 10 to 14 years of age. The results showed that there was no significant correlation between the systolic and diastolic pressure with the level of serum follicle-stimulating hormone and luteinizing hormone in either sex. Nor was there a significant correlation with the stage of pubic hair development in the boys nor with the stage of breast development, menarche, and duration since menarche in the girls. Therefore the presence of hypertension during 10 to 14 years of age should not be regarded as a physiologic response to pubertal development, but rather as a suspicious finding which deserves careful follow-up examinations.", "contents": "Blood pressure and puberty. The purpose of this investigation was to study the relation of blood pressure to sexual maturation in 229 boys and 189 girls, 10 to 14 years of age. The results showed that there was no significant correlation between the systolic and diastolic pressure with the level of serum follicle-stimulating hormone and luteinizing hormone in either sex. Nor was there a significant correlation with the stage of pubic hair development in the boys nor with the stage of breast development, menarche, and duration since menarche in the girls. Therefore the presence of hypertension during 10 to 14 years of age should not be regarded as a physiologic response to pubertal development, but rather as a suspicious finding which deserves careful follow-up examinations."}
{"id": "PMID:1081137", "title": "Portal hypertension in children following neonatal umbilical disorders.", "content": "10 children presented pre-hepatic portal vein obstruction during their first 6 years of life. 8 of them had massive esophageal varices, 1 died from acute esophageal hemorrhage. The perinatal history of these childre was studied: All of them had an abnormal birth history and all had been hospitalized during their neonatal period. In 5 of them an umbilical infection had been diagnosed, one had an injection of THAM and one other an exchange transfusion through an umbilical vein catheter. Pre-hepatic portal vein occlusion in children is presumed to be mainly an acquired disease resulting from neonatal umbilical disorders.", "contents": "Portal hypertension in children following neonatal umbilical disorders. 10 children presented pre-hepatic portal vein obstruction during their first 6 years of life. 8 of them had massive esophageal varices, 1 died from acute esophageal hemorrhage. The perinatal history of these childre was studied: All of them had an abnormal birth history and all had been hospitalized during their neonatal period. In 5 of them an umbilical infection had been diagnosed, one had an injection of THAM and one other an exchange transfusion through an umbilical vein catheter. Pre-hepatic portal vein occlusion in children is presumed to be mainly an acquired disease resulting from neonatal umbilical disorders."}
{"id": "PMID:1081138", "title": "Mechanism of frequency-dependent inhibition of sodium currents in frog myelinated nerve by the lidocaine derivative GEA.", "content": "A new lidocaine derivative (Astra, GEA 968) depresses excitability of myelinated frog nerve in a manner which depends upon the rate of use of the nerve. This phenomenon has been shown, under voltage clamp conditions, to involve \"frequency-\" or \"use-dependent\" inhibition of the transient inward sodium currents at the node of Ranvier. With 0.6 mM GEA 968 in the solution bathing the node, the inward sodium currents produced by 5-msec depolarizing pulses to -20 mV are reduced to 40% of control values if the node is rested for a few hundred seconds prior to the test pulse. Repetitive opening of the sodium channels by depolarizing pulses enhances this inhibition, for example, currents are eventually reduced to 10 to 20% of control with repetitive depolarization at 2 sec-1. If the preparation is then allowed to rest, this use-dependent increment in inhibition gradually declines with a time constant of about 10 seconds. Repetitive opening of the sodium channels by depolarizing pulses preceded by large hyperpolarizing prepulses reverses the inhibition caused by application of depolarizing pulses alone. It is hypothesized that the GEA 968 molecule binds to open sodium channels and, in doing so, simultaneously blocks the channel and shifts the curve relating sodium inactivation to membrane potential by 20 to 40 mV in the hyperpolarizing direction. Several kinds of evidence supporting this molecular hypothesis are presented. Lidocaine, procaine, procaine amide and a quaternary lidocaine derivative QX-314 also cause use-dependent depression of sodium currents in this preparation. This common mode of action of tertiary and quaternary anesthetics implies that the cationic form of tertiary anesthetics is active.", "contents": "Mechanism of frequency-dependent inhibition of sodium currents in frog myelinated nerve by the lidocaine derivative GEA. A new lidocaine derivative (Astra, GEA 968) depresses excitability of myelinated frog nerve in a manner which depends upon the rate of use of the nerve. This phenomenon has been shown, under voltage clamp conditions, to involve \"frequency-\" or \"use-dependent\" inhibition of the transient inward sodium currents at the node of Ranvier. With 0.6 mM GEA 968 in the solution bathing the node, the inward sodium currents produced by 5-msec depolarizing pulses to -20 mV are reduced to 40% of control values if the node is rested for a few hundred seconds prior to the test pulse. Repetitive opening of the sodium channels by depolarizing pulses enhances this inhibition, for example, currents are eventually reduced to 10 to 20% of control with repetitive depolarization at 2 sec-1. If the preparation is then allowed to rest, this use-dependent increment in inhibition gradually declines with a time constant of about 10 seconds. Repetitive opening of the sodium channels by depolarizing pulses preceded by large hyperpolarizing prepulses reverses the inhibition caused by application of depolarizing pulses alone. It is hypothesized that the GEA 968 molecule binds to open sodium channels and, in doing so, simultaneously blocks the channel and shifts the curve relating sodium inactivation to membrane potential by 20 to 40 mV in the hyperpolarizing direction. Several kinds of evidence supporting this molecular hypothesis are presented. Lidocaine, procaine, procaine amide and a quaternary lidocaine derivative QX-314 also cause use-dependent depression of sodium currents in this preparation. This common mode of action of tertiary and quaternary anesthetics implies that the cationic form of tertiary anesthetics is active."}
{"id": "PMID:1081139", "title": "Voltage dependence of agonist effectiveness at the frog neuromuscular junction: resolution of a paradox.", "content": "1. End-plate currents produced by nerve-released acetylcholine and iontophoretically applied acetylcholine and carbachol have been recorded from voltage-clamped frog cutaneous pectoris neuromuscular junctions made visible with Nomarski differential interference contrast optics. 2. The effectiveness of agonists - that is, the end-plate conductance change produced by a given dose-has been determined as a function of post-junctional membrane potential. 3. As the post-junctional membrane potential is made more negative, nerve-released acetylcholine becomes less effective whereas iontophoretically-applied agonists become more effective. 4. This voltage dependence of agonist effectiveness is mediated neither by end-plate current iontophoresis of agonist into the cleft nor through electric field effects on the esterase. 5. Influences of membrane potential on the opening and closing of end-plate channel gates can account quantitatively for the voltage-dependent effectiveness of both nerve-released and iontophoretically applied agonist.", "contents": "Voltage dependence of agonist effectiveness at the frog neuromuscular junction: resolution of a paradox. 1. End-plate currents produced by nerve-released acetylcholine and iontophoretically applied acetylcholine and carbachol have been recorded from voltage-clamped frog cutaneous pectoris neuromuscular junctions made visible with Nomarski differential interference contrast optics. 2. The effectiveness of agonists - that is, the end-plate conductance change produced by a given dose-has been determined as a function of post-junctional membrane potential. 3. As the post-junctional membrane potential is made more negative, nerve-released acetylcholine becomes less effective whereas iontophoretically-applied agonists become more effective. 4. This voltage dependence of agonist effectiveness is mediated neither by end-plate current iontophoresis of agonist into the cleft nor through electric field effects on the esterase. 5. Influences of membrane potential on the opening and closing of end-plate channel gates can account quantitatively for the voltage-dependent effectiveness of both nerve-released and iontophoretically applied agonist."}
{"id": "PMID:1081140", "title": "Intracellular chloride activity in frog heart.", "content": "1. Chloride liquid ion exchanger micro-electrodes have been used to measure intracellular chloride activity (aiCl) in isolated pieces of frog sinus venosus, atrium and ventricle. 2. aiCl was found to be 35-0 +/- 3-2, 17-3 +/- 0-8 and 17-6 +/- 0-8 mM in sinus venosus, atrium and ventricle, respectively. 3. In atrium and ventricle aiCl is independent of whether the tissue is beating or non-beating. 4. In non-beating ventricle aiCl changes rapidly and reversibly when aOCl is reduced by replacing chloride with glucuronate. 5. ECl calculated from the measured aiCl has been compared with ECl calculated from the constant field equation assuming either constant chloride conductance or constant chloride permeability. 6. It is suggested that chloride is actively transported into the cells in all three kinds of frog heart tissue.", "contents": "Intracellular chloride activity in frog heart. 1. Chloride liquid ion exchanger micro-electrodes have been used to measure intracellular chloride activity (aiCl) in isolated pieces of frog sinus venosus, atrium and ventricle. 2. aiCl was found to be 35-0 +/- 3-2, 17-3 +/- 0-8 and 17-6 +/- 0-8 mM in sinus venosus, atrium and ventricle, respectively. 3. In atrium and ventricle aiCl is independent of whether the tissue is beating or non-beating. 4. In non-beating ventricle aiCl changes rapidly and reversibly when aOCl is reduced by replacing chloride with glucuronate. 5. ECl calculated from the measured aiCl has been compared with ECl calculated from the constant field equation assuming either constant chloride conductance or constant chloride permeability. 6. It is suggested that chloride is actively transported into the cells in all three kinds of frog heart tissue."}
{"id": "PMID:1081141", "title": "The effect of zinc ions on the gating of the delayed potassium conductance of frog sartorius muscle.", "content": "1. A voltage-clamp method was used to examine the effects of zinc ions on the delayed potassium currents and also the slowly activating potassium currents that are turned on by depolarizing the membrane of frog sartorius muscle fibres. 2. In a control solution, the delayed potassium conductance had a maximum value of 17-8 +/- 2-5 mmho.cm-2. The reversal potential for the currents was -76-9 +/- 2-5 mV. The membrane potential where ninfinity had the value 0-5 was -49 mV. 3. The major effect of zinc ions was to slow the delayed potassium currents. The value of taun was increased approximately tenfold in 0-1 mM zinc. Zinc does not alter the effective valency of the gating particles of the potassium channel, but the conductance was shifted to more positive membrane potentials: in 0-1 mM zinc, the membrane potential where ninfinity had the value 0-5 was -32 mV. 4. Zinc ions, at a concentration of 0-1 mM, also reduced the maximum potassium conductance by about 60% to 7-3 +/- 0-8 mmho, cm-2; they did not alter the reversal potential of the currents, which had a value in 0-1 mM zinc of -74-6 +/- 1-5 mV. 5. Zinc ions had little or no effect on the rate of inactivation of the potassium currents. 6. Zinc ions had little effect on the conductance attributable to the slowly activating potassium system. In 0-01 mM zinc this conductance had a value at 0 to +10 mV of 1-25 +/- 0-29 mmho.cm-2. Zinc did not alter the reversal potential of the slow potassium currents from the value of -85 +/- 1-6 mV in the absence of zinc and had no effect on the time course of the turn-off of these currents at -60 mV. 7. The delayed potassium currents obtained in 0-002 and 0-01 mM zinc could not be fitted exactly with a simple fourth order equation, but were well fitted by a model proposing that zinc ions slow the opening and closing of the gating mechanism to one tenth the normal rate when they bind to the gating molecule. If the binding sites are not saturated, those gating molecules that do not bind zinc are assumed to be quite unaltered in their properties, though the potential dependence of their rate constants alphan and betan was assumed to be shifted to more positive levels. In one fibre in 0-01 mM zinc, the model fitted the currents best if 50% of the gating molecules bound zinc.", "contents": "The effect of zinc ions on the gating of the delayed potassium conductance of frog sartorius muscle. 1. A voltage-clamp method was used to examine the effects of zinc ions on the delayed potassium currents and also the slowly activating potassium currents that are turned on by depolarizing the membrane of frog sartorius muscle fibres. 2. In a control solution, the delayed potassium conductance had a maximum value of 17-8 +/- 2-5 mmho.cm-2. The reversal potential for the currents was -76-9 +/- 2-5 mV. The membrane potential where ninfinity had the value 0-5 was -49 mV. 3. The major effect of zinc ions was to slow the delayed potassium currents. The value of taun was increased approximately tenfold in 0-1 mM zinc. Zinc does not alter the effective valency of the gating particles of the potassium channel, but the conductance was shifted to more positive membrane potentials: in 0-1 mM zinc, the membrane potential where ninfinity had the value 0-5 was -32 mV. 4. Zinc ions, at a concentration of 0-1 mM, also reduced the maximum potassium conductance by about 60% to 7-3 +/- 0-8 mmho, cm-2; they did not alter the reversal potential of the currents, which had a value in 0-1 mM zinc of -74-6 +/- 1-5 mV. 5. Zinc ions had little or no effect on the rate of inactivation of the potassium currents. 6. Zinc ions had little effect on the conductance attributable to the slowly activating potassium system. In 0-01 mM zinc this conductance had a value at 0 to +10 mV of 1-25 +/- 0-29 mmho.cm-2. Zinc did not alter the reversal potential of the slow potassium currents from the value of -85 +/- 1-6 mV in the absence of zinc and had no effect on the time course of the turn-off of these currents at -60 mV. 7. The delayed potassium currents obtained in 0-002 and 0-01 mM zinc could not be fitted exactly with a simple fourth order equation, but were well fitted by a model proposing that zinc ions slow the opening and closing of the gating mechanism to one tenth the normal rate when they bind to the gating molecule. If the binding sites are not saturated, those gating molecules that do not bind zinc are assumed to be quite unaltered in their properties, though the potential dependence of their rate constants alphan and betan was assumed to be shifted to more positive levels. In one fibre in 0-01 mM zinc, the model fitted the currents best if 50% of the gating molecules bound zinc."}
{"id": "PMID:1081143", "title": "Circulating T and B lymphocytes in progressive systemic sclerosis.", "content": "Thymus derived (T) and bone-marrow derived (B) lymphocytes were quantitated in 11 untreated patients with progressive systemic sclerosis (PSS) and in 11 age and sex matched controls. T and B lymphocytes were identified according to their receptor sites for sheep red blood cells and activated complement, respectively. All patients had a normal lymphocyte count. The mean per cent value of circulating T lymphocytes was less in PSS patients (38 per cent) as compared with controls (56 per cent) (P less than 0.005), while no significant difference existed for the circulating B lymphocytes (PSS 14 per cent; controls 11 per cent). The mitogenic response to phytohemagglutinin (PHA) was assessed by comparing dose response curves in PSS with those obtained from matched controls. No significant difference could be detected between these groups.", "contents": "Circulating T and B lymphocytes in progressive systemic sclerosis. Thymus derived (T) and bone-marrow derived (B) lymphocytes were quantitated in 11 untreated patients with progressive systemic sclerosis (PSS) and in 11 age and sex matched controls. T and B lymphocytes were identified according to their receptor sites for sheep red blood cells and activated complement, respectively. All patients had a normal lymphocyte count. The mean per cent value of circulating T lymphocytes was less in PSS patients (38 per cent) as compared with controls (56 per cent) (P less than 0.005), while no significant difference existed for the circulating B lymphocytes (PSS 14 per cent; controls 11 per cent). The mitogenic response to phytohemagglutinin (PHA) was assessed by comparing dose response curves in PSS with those obtained from matched controls. No significant difference could be detected between these groups."}
{"id": "PMID:1081163", "title": "A genetic-epidemiologic study of chronic obstructive pulmonary disease. I. Study design and preliminary observations.", "content": "Although certain environmental agents (e.g., cigarette smoking) are known to be causally related to chronic obstructive pulmonary disease (COPD), differential response to their deleterious effects suggests the importance of constitutional (host) factors. The voluminous literature on the familial occurrence of COPD as well as the association between genetically determined serum alpha 1-antitrypsin (alpha 1-at) deficiency and COPD, however, reveals many aspects yet to be clarified. Studies of alpha 1-at indicate that neither its nature nor its relationship to COPD is simple. Moreover, other familial factors are likely involved. To obtain a better understanding of the etiology and pathogenesis of COPD, both genetic and environmental factors are being examined in a multifaceted investigation. The preliminary observations are summarized. COPD patients have a higher frequency of Pi variant phenotypes than those without lung disease. Among other subjects, both cigarette smoking and Pi variant phenotypes are associated with increased pulmonary function abnormality. Finally, there is familial aggregation of pulmonary impairment that cannot be explained entirely by Pi type or smoking.", "contents": "A genetic-epidemiologic study of chronic obstructive pulmonary disease. I. Study design and preliminary observations. Although certain environmental agents (e.g., cigarette smoking) are known to be causally related to chronic obstructive pulmonary disease (COPD), differential response to their deleterious effects suggests the importance of constitutional (host) factors. The voluminous literature on the familial occurrence of COPD as well as the association between genetically determined serum alpha 1-antitrypsin (alpha 1-at) deficiency and COPD, however, reveals many aspects yet to be clarified. Studies of alpha 1-at indicate that neither its nature nor its relationship to COPD is simple. Moreover, other familial factors are likely involved. To obtain a better understanding of the etiology and pathogenesis of COPD, both genetic and environmental factors are being examined in a multifaceted investigation. The preliminary observations are summarized. COPD patients have a higher frequency of Pi variant phenotypes than those without lung disease. Among other subjects, both cigarette smoking and Pi variant phenotypes are associated with increased pulmonary function abnormality. Finally, there is familial aggregation of pulmonary impairment that cannot be explained entirely by Pi type or smoking."}
{"id": "PMID:1081164", "title": "Surface markers of human lymphocytes.", "content": "The large accumulation of data on serum immunoglobulins (Igs) has made it possible to delineate readily the special membrane Ig receptors that respond to antigenic stimulation. B type lymphocytes are characterized by the selective presence of IgM and IgD. IgG, the main serum Ig, is rarely found and its previous reported presence was due to secondary adsorption. IgM and IgD occur together on the same lymphocytes and show similar or identical variable regions and antigen combining sites. The membrane Igs are positioned on the lymphocyte surface with a cosiderable part of the C-terminal portion of the heavy chains buried in the membrane. In addition to numerous other lymphocyte membrane markers, recent interest has centered on the new histocompatibility surface antigen, HL-B, which appears related to immune response genes and is selectively represented on B cells. Through the use of this group of lymphocyte markers considerable information on the character of the malignant cells in various leukemias and lymphomas has been obtained. Chronic lymphatic leukemia is essentially a proliferation of B cells. Acute lymphoblastic leukemia falls into two groups, a minor type that represents a proliferation of T cells and a major type that appears to show an immature cell type of probable B cells lineage. Various immunodeficiency syndromes have been clarified considerably through lymphocyte analyses and certain defects have been determined.", "contents": "Surface markers of human lymphocytes. The large accumulation of data on serum immunoglobulins (Igs) has made it possible to delineate readily the special membrane Ig receptors that respond to antigenic stimulation. B type lymphocytes are characterized by the selective presence of IgM and IgD. IgG, the main serum Ig, is rarely found and its previous reported presence was due to secondary adsorption. IgM and IgD occur together on the same lymphocytes and show similar or identical variable regions and antigen combining sites. The membrane Igs are positioned on the lymphocyte surface with a cosiderable part of the C-terminal portion of the heavy chains buried in the membrane. In addition to numerous other lymphocyte membrane markers, recent interest has centered on the new histocompatibility surface antigen, HL-B, which appears related to immune response genes and is selectively represented on B cells. Through the use of this group of lymphocyte markers considerable information on the character of the malignant cells in various leukemias and lymphomas has been obtained. Chronic lymphatic leukemia is essentially a proliferation of B cells. Acute lymphoblastic leukemia falls into two groups, a minor type that represents a proliferation of T cells and a major type that appears to show an immature cell type of probable B cells lineage. Various immunodeficiency syndromes have been clarified considerably through lymphocyte analyses and certain defects have been determined."}
{"id": "PMID:1081167", "title": "Pathogenesis of one variant of sea-blue histiocytosis.", "content": "Hematopoietic cells from three siblings of a family with sea-blue histiocytosis were studied using in vitro cell culture techniques, histochemistry, and electron microscopy. Sphingomyelinase activities in cultured fibroblasts derived from these patients averaged 18 per cent of control levels. Ultrastructural examination of the sea-blue histiocytes showed numerous acid phosphatase-containing inclusions with closely packed, concentrically arranged, membrane-like lamellae with a periodicity of approximately 45 A. The inclusions are thought to result from the spatial orientation of complex lipid molecules and water. These structures are consistent with secondary lysosomes and are similar to those seen in some cases of adult Niemann-Pick disease and other cases of sea-blue histiocytosis. Extraction of lipid did not alter the blue-staining characteristics of the histiocytes. Sea-blue histiocytes were seen in liquid bone marrow cultures but did not proliferate, suggesting that the mature sea-blue macrophage is a terminal cell. We propose that partial sphingomyelinase deficiency may be one cause of sea-blud histiocytosis.", "contents": "Pathogenesis of one variant of sea-blue histiocytosis. Hematopoietic cells from three siblings of a family with sea-blue histiocytosis were studied using in vitro cell culture techniques, histochemistry, and electron microscopy. Sphingomyelinase activities in cultured fibroblasts derived from these patients averaged 18 per cent of control levels. Ultrastructural examination of the sea-blue histiocytes showed numerous acid phosphatase-containing inclusions with closely packed, concentrically arranged, membrane-like lamellae with a periodicity of approximately 45 A. The inclusions are thought to result from the spatial orientation of complex lipid molecules and water. These structures are consistent with secondary lysosomes and are similar to those seen in some cases of adult Niemann-Pick disease and other cases of sea-blue histiocytosis. Extraction of lipid did not alter the blue-staining characteristics of the histiocytes. Sea-blue histiocytes were seen in liquid bone marrow cultures but did not proliferate, suggesting that the mature sea-blue macrophage is a terminal cell. We propose that partial sphingomyelinase deficiency may be one cause of sea-blud histiocytosis."}
{"id": "PMID:1081169", "title": "Long-term functional results of surgery for coronary artery disease in patients with poor ventricular function.", "content": "Thirty-two patients with coronary artery disease and an ejection fraction (EF) below 0.40 had coronary bypass and/or left ventricular aneurysm (LVA) resection. Twenty-four patients had a previous infarction, 12 had congestive heart failure (CHF), and left ventricular end-diastolic pressure averaged 15 mm. Hg. Nineteen patients had three-vessel involvement and there was an  average of 2.4 vessels involved per patient. Bypass was accomplished on 87 per cent of vessels; 11 patients had resection of LVA; 11 had coronary endarterectomy. There were no hospital deaths, 63 per cent of the patients were improved, and 56 per cent were able to return to work or full activity. There were five late deaths (16 per cent). There was good functional improvement in 83 per cent of the survivors, followed 1 to 4 years. We believe that patients with poor ventricular function due to severe coronary artery disease should not categorically be denied surgery, and that operation can be done with a low hospital mortality rate and good functional results.", "contents": "Long-term functional results of surgery for coronary artery disease in patients with poor ventricular function. Thirty-two patients with coronary artery disease and an ejection fraction (EF) below 0.40 had coronary bypass and/or left ventricular aneurysm (LVA) resection. Twenty-four patients had a previous infarction, 12 had congestive heart failure (CHF), and left ventricular end-diastolic pressure averaged 15 mm. Hg. Nineteen patients had three-vessel involvement and there was an  average of 2.4 vessels involved per patient. Bypass was accomplished on 87 per cent of vessels; 11 patients had resection of LVA; 11 had coronary endarterectomy. There were no hospital deaths, 63 per cent of the patients were improved, and 56 per cent were able to return to work or full activity. There were five late deaths (16 per cent). There was good functional improvement in 83 per cent of the survivors, followed 1 to 4 years. We believe that patients with poor ventricular function due to severe coronary artery disease should not categorically be denied surgery, and that operation can be done with a low hospital mortality rate and good functional results."}
{"id": "PMID:1081170", "title": "The superiority of aortic cross-clamping with profound local hypothermia for myocardial protection during aorta-coronary bypass grafting.", "content": "Two different operative techniques for aorta-coronary bypass grafting were utilized in two comparable groups of patients. In one group (155 patients) distal anastomoses were carried out with the aorta cross-clamped and myocardial protection provided by profound local hypothermia (clamped group). In a second group (149 patients) distal anastomoses were carried out with the aorta unclamped and the left ventricle fibrillating and vented (unclamped group). Mortality rates were similar in the two groups (0.6 per cent in clamped group versus 1.3 per cent in unclamped group). The incidence of perioperative infarction was 15 per cent in the unclamped group and 8 per cent in the clamped group (p less than 0.05). Postoperative serum glutamic oxaloacetic transaminase (SGOT) and lactic dehydrogenase (LDH) levels were significantly higher for the first 4 postoperative days in the unclamped group than in the clamped group. Hemodynamic studies in a subset of each group revealed no important differences in left ventricular function in the immediate postoperative period. The data demonstrate that in patients undergoing aorta coronary bypass grafting, performance of distal anastomoses with aortic cross-clamping and profound local hypothermia results in less intraoperative myocardial injury than performance of distal anastomoses in the perfused, fibrillating, and vented left ventricle.", "contents": "The superiority of aortic cross-clamping with profound local hypothermia for myocardial protection during aorta-coronary bypass grafting. Two different operative techniques for aorta-coronary bypass grafting were utilized in two comparable groups of patients. In one group (155 patients) distal anastomoses were carried out with the aorta cross-clamped and myocardial protection provided by profound local hypothermia (clamped group). In a second group (149 patients) distal anastomoses were carried out with the aorta unclamped and the left ventricle fibrillating and vented (unclamped group). Mortality rates were similar in the two groups (0.6 per cent in clamped group versus 1.3 per cent in unclamped group). The incidence of perioperative infarction was 15 per cent in the unclamped group and 8 per cent in the clamped group (p less than 0.05). Postoperative serum glutamic oxaloacetic transaminase (SGOT) and lactic dehydrogenase (LDH) levels were significantly higher for the first 4 postoperative days in the unclamped group than in the clamped group. Hemodynamic studies in a subset of each group revealed no important differences in left ventricular function in the immediate postoperative period. The data demonstrate that in patients undergoing aorta coronary bypass grafting, performance of distal anastomoses with aortic cross-clamping and profound local hypothermia results in less intraoperative myocardial injury than performance of distal anastomoses in the perfused, fibrillating, and vented left ventricle."}
{"id": "PMID:1081171", "title": "Recent progress in mycoplasma research.", "content": "Today, 12 years after the identification of Eaton agent as a mycoplasma, much new information on this infection has accumulated. Better methods have become available, which will be helpful in determining whether other mycoplasmas might be human pathogens, e.g., some ureaplasmas in nongonococcal urethritis. Experimental mycoplasma infections in birds and animals have revealed common features with immune complex diseases in man. They may serve, therefore, as useful models for human mycoplasma disease. However, the most important finding in recent mycoplasma research has been the isolation of mycoplasma viruses. Viruses belonging to three different groups have been discovered. It will be interesting to study whether the virus determines the pathogenicity of mycoplasmas or not.", "contents": "Recent progress in mycoplasma research. Today, 12 years after the identification of Eaton agent as a mycoplasma, much new information on this infection has accumulated. Better methods have become available, which will be helpful in determining whether other mycoplasmas might be human pathogens, e.g., some ureaplasmas in nongonococcal urethritis. Experimental mycoplasma infections in birds and animals have revealed common features with immune complex diseases in man. They may serve, therefore, as useful models for human mycoplasma disease. However, the most important finding in recent mycoplasma research has been the isolation of mycoplasma viruses. Viruses belonging to three different groups have been discovered. It will be interesting to study whether the virus determines the pathogenicity of mycoplasmas or not."}
{"id": "PMID:1081181", "title": "A quantitative approach to evaluating the effect of stimulating devices on the perception of noxious stimuli.", "content": "A new type of dolorimeter has been used in determining the nature of the mechanism responsible for the analgesic effect produced by electrostimulating devices. A study in normal subjects, yielding quantitative data on this analgesic effect, is reported.", "contents": "A quantitative approach to evaluating the effect of stimulating devices on the perception of noxious stimuli. A new type of dolorimeter has been used in determining the nature of the mechanism responsible for the analgesic effect produced by electrostimulating devices. A study in normal subjects, yielding quantitative data on this analgesic effect, is reported."}
{"id": "PMID:1081182", "title": "Neurostimulation of the celiac plexus.", "content": "Effective relief of abdominal pain lasting from 2 hours to 25 days was achieved in eight patients by neurostimulation of the celiac plexus. The procedure is judged to be superior to the nerve block.", "contents": "Neurostimulation of the celiac plexus. Effective relief of abdominal pain lasting from 2 hours to 25 days was achieved in eight patients by neurostimulation of the celiac plexus. The procedure is judged to be superior to the nerve block."}
{"id": "PMID:1081183", "title": "The viability of external electrical stimulation as a therapeutic modality.", "content": "This study indicates that external electrical stimulation is a valuable tool for control of acute pain in about 80 percent of patients but, for significant relief of pain, in only about 25 percent of patients with chronic pain. The author recognizes that in order to increase its use, a system for delivery of this therapy under a doctor's prescription, rather than having to teach each individual practitioner the technique for using the device, must be developed. In chronic-pain patients when external electrical stimulation is inadequate, a more aggressive and comprehensive program of operant conditioning, progressive physical exercise, drug withdrawal, autogenic training, and biofeedback is advised.", "contents": "The viability of external electrical stimulation as a therapeutic modality. This study indicates that external electrical stimulation is a valuable tool for control of acute pain in about 80 percent of patients but, for significant relief of pain, in only about 25 percent of patients with chronic pain. The author recognizes that in order to increase its use, a system for delivery of this therapy under a doctor's prescription, rather than having to teach each individual practitioner the technique for using the device, must be developed. In chronic-pain patients when external electrical stimulation is inadequate, a more aggressive and comprehensive program of operant conditioning, progressive physical exercise, drug withdrawal, autogenic training, and biofeedback is advised."}
{"id": "PMID:1081184", "title": "Innovations in neurologic implant systems.", "content": "Physiologic findings and clinical observations with several new implant systems are discussed. One system studied is designed with electrodes implanted over the anterior region of the spinal cord. Another system is designed with an anterior-posterior spinal cord electrode passing current across the spinal cord. Also given are observation and description of cortical and cerebellar implant systems. Current-density plots of the electrode arrays are included.", "contents": "Innovations in neurologic implant systems. Physiologic findings and clinical observations with several new implant systems are discussed. One system studied is designed with electrodes implanted over the anterior region of the spinal cord. Another system is designed with an anterior-posterior spinal cord electrode passing current across the spinal cord. Also given are observation and description of cortical and cerebellar implant systems. Current-density plots of the electrode arrays are included."}
{"id": "PMID:1081185", "title": "Experiences with electrical stimulation devices for the control of chronic pain.", "content": "The results of a study involving the treatment of pain syndromes by electrical stimulation in 98 patients are reported. Advantages and disadvantages of three electrostimulation techniques--transcutaneous stimulation, peripheral nerve stimulation, and dorsal cord stimulation--are discussed. Also included is a brief discussion of the possible mechanism of suppression of pain stimuli.", "contents": "Experiences with electrical stimulation devices for the control of chronic pain. The results of a study involving the treatment of pain syndromes by electrical stimulation in 98 patients are reported. Advantages and disadvantages of three electrostimulation techniques--transcutaneous stimulation, peripheral nerve stimulation, and dorsal cord stimulation--are discussed. Also included is a brief discussion of the possible mechanism of suppression of pain stimuli."}
{"id": "PMID:1081186", "title": "Implanted devices for electronic augmentation of nervous system function.", "content": "Electrical stimulation of the dorsal cord is discussed as a mode of therapy for intractable pain, and placed into its proper perspective as a part of a comprehensive rehabilitation program. The author reviews his experience with electrical stimulation and emphasizes features of the patient-selection process.", "contents": "Implanted devices for electronic augmentation of nervous system function. Electrical stimulation of the dorsal cord is discussed as a mode of therapy for intractable pain, and placed into its proper perspective as a part of a comprehensive rehabilitation program. The author reviews his experience with electrical stimulation and emphasizes features of the patient-selection process."}
{"id": "PMID:1081187", "title": "Electrical stimulation of peripheral nerves for relief of intractable chronic pain.", "content": "Electrostimulation of peripheral nerves was used in patients to relieve chronic pain due to nerve injury. After follow-up for periods of 6 to 48 months, most of the patients exhibited some degree of pain alleviation. This paper details the circumstances of some of the cases and cites precautions to be taken in the use of this therapeutic mode.", "contents": "Electrical stimulation of peripheral nerves for relief of intractable chronic pain. Electrostimulation of peripheral nerves was used in patients to relieve chronic pain due to nerve injury. After follow-up for periods of 6 to 48 months, most of the patients exhibited some degree of pain alleviation. This paper details the circumstances of some of the cases and cites precautions to be taken in the use of this therapeutic mode."}
{"id": "PMID:1081203", "title": "Effects of furosemide on sodium content and transport pool in frog skin (Rana esculenta): comparison with vasopressin and ouabain.", "content": "The effects of ouabain, vasopressin, and furosemide on intracellular concentrations of total sodium([Na]) and potassium [K]), on exchangeable sodium ([Na]) and the sodium transport pool ([Nap]) were investigated in isolated short circuited skins of rana esculenta. Furosemide was added to the epithelial bathing solution, vasopressin and ouabain to the corial bathing solution. Results were compared with the amount of net sodium transport measured by short circuit current (scc). Ouabain reduces scc and increases [Na] and [Na]; [K] is decreased. The administration of vasopressin leads to a sharp increase of scc, combined with an enhancement of of [Na] and [Na]; [K] shows no significant change. [Nap] is significantly increased, too, and approximately to the same amount as [Na]. Furosemide causes an increase of scc, whereas a significant change of [Na], [Na] and [K] could not be detected. On the other hand, [Nap] was enhanced significantly. The results support the hypothesis that furosemide like vasopressin is acting by increasing the entry of sodium into the transport compartment of the active cell layer. The result is an increased transfer of sodium across the skin.", "contents": "Effects of furosemide on sodium content and transport pool in frog skin (Rana esculenta): comparison with vasopressin and ouabain. The effects of ouabain, vasopressin, and furosemide on intracellular concentrations of total sodium([Na]) and potassium [K]), on exchangeable sodium ([Na]) and the sodium transport pool ([Nap]) were investigated in isolated short circuited skins of rana esculenta. Furosemide was added to the epithelial bathing solution, vasopressin and ouabain to the corial bathing solution. Results were compared with the amount of net sodium transport measured by short circuit current (scc). Ouabain reduces scc and increases [Na] and [Na]; [K] is decreased. The administration of vasopressin leads to a sharp increase of scc, combined with an enhancement of of [Na] and [Na]; [K] shows no significant change. [Nap] is significantly increased, too, and approximately to the same amount as [Na]. Furosemide causes an increase of scc, whereas a significant change of [Na], [Na] and [K] could not be detected. On the other hand, [Nap] was enhanced significantly. The results support the hypothesis that furosemide like vasopressin is acting by increasing the entry of sodium into the transport compartment of the active cell layer. The result is an increased transfer of sodium across the skin."}
{"id": "PMID:1081204", "title": "Air filling of the subarachnoid space over the cerebral convexities at repeat encephalography.", "content": "In order to check the constancy of convexity block, the airencephalograms (AEG) of seventeen patients who had undergone two separate examinations were reexamined. A satisfactory filling by air of the basal cisterns or Sylvian fissures was a prerequisite for including the AEG in this series. Consistent results related to air filling of the parietal region were obtained at the two examinations except in one patient with clinical signs of a pontine neoplasm. When the AEG was repeated in a very short time and with unchanged clinical conditions, the degree of air filling over the convexity was practically identical. But, with progressive neurological signs, the parietal air blocks showed a slight increase and also extended to the frontal region. It was concluded, firstly, that upper convexity block is a reproducible finding; secondly, that it is related to organic brain disease, the extent of the convexity block being correlated to the degree of clinical deterioration. As judged from other studies using isotope cisternography, the subarachnoid space is usually not complelely obstructed. It is suggested that the organically changed brain and leptomeninges create special physical conditions for the appearance of convexity air block in the AEG.", "contents": "Air filling of the subarachnoid space over the cerebral convexities at repeat encephalography. In order to check the constancy of convexity block, the airencephalograms (AEG) of seventeen patients who had undergone two separate examinations were reexamined. A satisfactory filling by air of the basal cisterns or Sylvian fissures was a prerequisite for including the AEG in this series. Consistent results related to air filling of the parietal region were obtained at the two examinations except in one patient with clinical signs of a pontine neoplasm. When the AEG was repeated in a very short time and with unchanged clinical conditions, the degree of air filling over the convexity was practically identical. But, with progressive neurological signs, the parietal air blocks showed a slight increase and also extended to the frontal region. It was concluded, firstly, that upper convexity block is a reproducible finding; secondly, that it is related to organic brain disease, the extent of the convexity block being correlated to the degree of clinical deterioration. As judged from other studies using isotope cisternography, the subarachnoid space is usually not complelely obstructed. It is suggested that the organically changed brain and leptomeninges create special physical conditions for the appearance of convexity air block in the AEG."}
{"id": "PMID:1081205", "title": "Experience with Dimer-X 1-ventriculography.", "content": "Positive contrast ventriculography was carried out in 72 cases, using the water-soluble contrast medium Dimer-X. The examinations were performed 1--3 days after ventricular drainage had been carried out to relieve raised intracranila pressure. After injection of 2--7.5 ml. of the medium into the non-anaesthetised patients -- including 37 children and juveniles -- the ventriculographs were taken under visual control using Mimer III. This method is simple and can be rapidly carried out. Radiographs taken in two planes, supplement by tomography if required, are sufficient for an accurate diagnosis. During the examination only two children suffered from vomiting, while another patient sustained a generalised convulsion because the contrast agent came into contact with the surface of the brain.", "contents": "Experience with Dimer-X 1-ventriculography. Positive contrast ventriculography was carried out in 72 cases, using the water-soluble contrast medium Dimer-X. The examinations were performed 1--3 days after ventricular drainage had been carried out to relieve raised intracranila pressure. After injection of 2--7.5 ml. of the medium into the non-anaesthetised patients -- including 37 children and juveniles -- the ventriculographs were taken under visual control using Mimer III. This method is simple and can be rapidly carried out. Radiographs taken in two planes, supplement by tomography if required, are sufficient for an accurate diagnosis. During the examination only two children suffered from vomiting, while another patient sustained a generalised convulsion because the contrast agent came into contact with the surface of the brain."}
{"id": "PMID:1081206", "title": "[Hiatal hernia during a course of indirect anticoagulant treatment].", "content": "A personal case of haematemesis in a patient subjected to indirect management with anticoagulants is reported. The prothrombin values were not within the danger limits. Some concise notes on the points raised by this finding are presented and essential features of the diagnosis and treatment are discussed.", "contents": "[Hiatal hernia during a course of indirect anticoagulant treatment]. A personal case of haematemesis in a patient subjected to indirect management with anticoagulants is reported. The prothrombin values were not within the danger limits. Some concise notes on the points raised by this finding are presented and essential features of the diagnosis and treatment are discussed."}
{"id": "PMID:1081207", "title": "Prevalence of coronary heart disease in samples of New Zealand Maoris and Pakehas.", "content": "This paper reports the prevalence of coronary heart disease (CHD) and its relationship with several standard risk factors in samples of New Zealand Maoris and Pakehas. The age standardised prevalence rates of CHD are: 16.1 percent, and 7.3 percent in Maori females and males respectively, and 11.5 percent and 6.5 percent in Pakeha females and males respectively. In Maori females only serum uric acid is associated with an increasing prevalence of CHD. The high prevalence of CHD in females as compared with the higher national mortality rates for males suggests that the case fatality rates for CHD are higher in males. The lack of association between CHD and the risk factors in Maori females suggests the possibility of two syndromes of CHD in Maori females.", "contents": "Prevalence of coronary heart disease in samples of New Zealand Maoris and Pakehas. This paper reports the prevalence of coronary heart disease (CHD) and its relationship with several standard risk factors in samples of New Zealand Maoris and Pakehas. The age standardised prevalence rates of CHD are: 16.1 percent, and 7.3 percent in Maori females and males respectively, and 11.5 percent and 6.5 percent in Pakeha females and males respectively. In Maori females only serum uric acid is associated with an increasing prevalence of CHD. The high prevalence of CHD in females as compared with the higher national mortality rates for males suggests that the case fatality rates for CHD are higher in males. The lack of association between CHD and the risk factors in Maori females suggests the possibility of two syndromes of CHD in Maori females."}
{"id": "PMID:1081208", "title": "Acute pancreatitis ten years experience in the Waikato district.", "content": "Seventy patients admitted to Waikato Hospital between 1964-74 with acute pancreatitis have been reviewed. Biliary tract disease and alcohol are the most common aetiological agents. The disease is most common in middle age. Europeans and Polynesians have similar incidence rates. The diagnosis is frequently not made at admission and most admissions are in the afternoon or early evening. Radiology is helpful in the diagnosis although nonspecific. Abnormal biochemistry is discussed and related to mortality. Additional tests, serum catalase/methaemalbumin are promoted to assist in the diagnosis and indicating the severity of the disease. Glucagon and Trasylol are discussed as being beneficial in this disease and combination therapy is suggested. The role of surgery is discussed.", "contents": "Acute pancreatitis ten years experience in the Waikato district. Seventy patients admitted to Waikato Hospital between 1964-74 with acute pancreatitis have been reviewed. Biliary tract disease and alcohol are the most common aetiological agents. The disease is most common in middle age. Europeans and Polynesians have similar incidence rates. The diagnosis is frequently not made at admission and most admissions are in the afternoon or early evening. Radiology is helpful in the diagnosis although nonspecific. Abnormal biochemistry is discussed and related to mortality. Additional tests, serum catalase/methaemalbumin are promoted to assist in the diagnosis and indicating the severity of the disease. Glucagon and Trasylol are discussed as being beneficial in this disease and combination therapy is suggested. The role of surgery is discussed."}
{"id": "PMID:1081209", "title": "Four channel electronystagmographic (ENG) studies of eye movements induced by linear accelerations.", "content": "A parallel-swing ENG study in rabbits led to two investigative conclusions: (a) for experimental purposes the parallel swing test must be performed with monocular, both horizontal and vertical leads, and (b) such studies must be performed in a darkened room. Following surgical bilateral \"immobilization\" of the cupulae of the horizontal semicircular canals, horizontal nystagmus induced in the parallel-swing test is probably generated by the otolithic system.", "contents": "Four channel electronystagmographic (ENG) studies of eye movements induced by linear accelerations. A parallel-swing ENG study in rabbits led to two investigative conclusions: (a) for experimental purposes the parallel swing test must be performed with monocular, both horizontal and vertical leads, and (b) such studies must be performed in a darkened room. Following surgical bilateral \"immobilization\" of the cupulae of the horizontal semicircular canals, horizontal nystagmus induced in the parallel-swing test is probably generated by the otolithic system."}
{"id": "PMID:1081210", "title": "The circular eye-tracking test. I. Simultaneous recording of the horizontal and vertical component of eye movement in the eye-tracking test.", "content": "Almost all of the conventional clinical reports on the eye-tracking test are made on the horizontal component of the smooth pursuit function of oculomotor system; few are made on the vertical component. One reason is that during the conventional vertical eye-tracking test the artifacts caused by movement of the upper eyelids often make it difficult to analyze the data. To overcome this problem the authors have developed a method to record simultaneously the horizontal and vertical components of eye movement in the eye-tracking test, and moreover, to reduce artifacts as much as possible. Our method, the circular eye-tracking test, is performed by means of an apparatus that provides a visual stimulus drawing a circle clockwise, at a rate of 120 degrees/sec, with a radius of 25.5 cm, and viewed at a distance of 70 cm.", "contents": "The circular eye-tracking test. I. Simultaneous recording of the horizontal and vertical component of eye movement in the eye-tracking test. Almost all of the conventional clinical reports on the eye-tracking test are made on the horizontal component of the smooth pursuit function of oculomotor system; few are made on the vertical component. One reason is that during the conventional vertical eye-tracking test the artifacts caused by movement of the upper eyelids often make it difficult to analyze the data. To overcome this problem the authors have developed a method to record simultaneously the horizontal and vertical components of eye movement in the eye-tracking test, and moreover, to reduce artifacts as much as possible. Our method, the circular eye-tracking test, is performed by means of an apparatus that provides a visual stimulus drawing a circle clockwise, at a rate of 120 degrees/sec, with a radius of 25.5 cm, and viewed at a distance of 70 cm."}
{"id": "PMID:1081216", "title": "Conductance fluctuations in Ranvier nodes.", "content": "Voltage fluctuations associated with the sodium system were measured upon elimination of the potassium current in the nodal membrane by internal application of cesium-ions. The intensity of this noise reaches a maximal value at a membrane potential in the vicinity of --40 mV. Here the power spectrum consists of two additive components: a 1/f component and a Lorentzian. The Lorentzian is associated with h-gate kinetics and is consistent with the binary state conduction model. On the basis of this model the sodium-channel conductance is calculated to be 2 to 5-10(-12) S. The analysis is complicated by the existence of an incomplete slow sodium inactivation process.", "contents": "Conductance fluctuations in Ranvier nodes. Voltage fluctuations associated with the sodium system were measured upon elimination of the potassium current in the nodal membrane by internal application of cesium-ions. The intensity of this noise reaches a maximal value at a membrane potential in the vicinity of --40 mV. Here the power spectrum consists of two additive components: a 1/f component and a Lorentzian. The Lorentzian is associated with h-gate kinetics and is consistent with the binary state conduction model. On the basis of this model the sodium-channel conductance is calculated to be 2 to 5-10(-12) S. The analysis is complicated by the existence of an incomplete slow sodium inactivation process."}
{"id": "PMID:1081217", "title": "Deviating flux ratios for Na+ in ouabain-treated frog skin.", "content": "Unidirectional Na+ fluxes across ouabain-treated frog skins were measured at different applied voltages. The calculated influx/efflux ratios appear to deviate markedly from Ussing's flux-ratio equation. This means that interactions of Na+ ions with some component in the system occur. Possible mechanisms, responsible for this phenomenon, are indicated.", "contents": "Deviating flux ratios for Na+ in ouabain-treated frog skin. Unidirectional Na+ fluxes across ouabain-treated frog skins were measured at different applied voltages. The calculated influx/efflux ratios appear to deviate markedly from Ussing's flux-ratio equation. This means that interactions of Na+ ions with some component in the system occur. Possible mechanisms, responsible for this phenomenon, are indicated."}
{"id": "PMID:1081218", "title": "A study of desensitization using voltage clamp.", "content": "The onset and offset of desensitization produced by carbachol and hexyl and heptyl TMA at voltage-clamped frog endplates were studied. Desensitization onsets exponentially with a rate constant which is proportional to the agonist concentration. The proportionality constant varies with the agonist used. The plateau current finally attained falls steeply to low values with increasing agonist concentration, and with further increase in concentration varies relatively little. The offset of desensitization is sometimes accompanied by the appearance of a second transient increase in current. It is proposed that desensitization is due to channel blockade by the agonist molecule itself.", "contents": "A study of desensitization using voltage clamp. The onset and offset of desensitization produced by carbachol and hexyl and heptyl TMA at voltage-clamped frog endplates were studied. Desensitization onsets exponentially with a rate constant which is proportional to the agonist concentration. The proportionality constant varies with the agonist used. The plateau current finally attained falls steeply to low values with increasing agonist concentration, and with further increase in concentration varies relatively little. The offset of desensitization is sometimes accompanied by the appearance of a second transient increase in current. It is proposed that desensitization is due to channel blockade by the agonist molecule itself."}
{"id": "PMID:1081227", "title": "1-Pyrene-butyrylcholine: a fluorescent probe for the cholinergic system.", "content": "The action of 1-pyrene-butyrylcholine, a new cholinergic fluorescent probe, has been studied at the cellular level using electrophysiological and fluorescence techniques. The spectroscopic properties of the probe were found to be similar to those pf pyrene-butyric acid, the excited-state lifetime in air-saturated aqueous solutions being 92 nsec. At micromolar concentrations the probe was found to exert a nondepolarizing, reversible blocking action at the neuromuscular junction of the frog. The same cholinolytic effect was observed in hypersensitive denervated muscles. The synaptic localization of the probe could be observed with fluorescence microscopy using sub- and micromolar concentrations. Treatment of the nerve-muscle preparations with proteolytic enzymes, resulting in the separation of the nerve ending from the muscle end-plate, enabled a distinction to be made between the fluorescence arising from these two parts of the synapse. Intense presynaptic fluorescence was observed, and was not altered by micromolar concentrations of alpha-bungarotoxin, d-tubocurarine, hemicholinium, or cholinesterase inhibitors. Faint reversible staining of the end-plate region was observed in enzymically treated muscles and was inhibited by prior treatment with alpha-bungarotoxin. Fluorescent alpha-toxin revealed similar patterns of fluorescence in the end-plate of enzyme-treated muscles. The postsynaptic localization of the fluorescent probe is therefore tentatively identified as the one producing the cholinolytic effect upon binding to acetylcholine receptor sites.", "contents": "1-Pyrene-butyrylcholine: a fluorescent probe for the cholinergic system. The action of 1-pyrene-butyrylcholine, a new cholinergic fluorescent probe, has been studied at the cellular level using electrophysiological and fluorescence techniques. The spectroscopic properties of the probe were found to be similar to those pf pyrene-butyric acid, the excited-state lifetime in air-saturated aqueous solutions being 92 nsec. At micromolar concentrations the probe was found to exert a nondepolarizing, reversible blocking action at the neuromuscular junction of the frog. The same cholinolytic effect was observed in hypersensitive denervated muscles. The synaptic localization of the probe could be observed with fluorescence microscopy using sub- and micromolar concentrations. Treatment of the nerve-muscle preparations with proteolytic enzymes, resulting in the separation of the nerve ending from the muscle end-plate, enabled a distinction to be made between the fluorescence arising from these two parts of the synapse. Intense presynaptic fluorescence was observed, and was not altered by micromolar concentrations of alpha-bungarotoxin, d-tubocurarine, hemicholinium, or cholinesterase inhibitors. Faint reversible staining of the end-plate region was observed in enzymically treated muscles and was inhibited by prior treatment with alpha-bungarotoxin. Fluorescent alpha-toxin revealed similar patterns of fluorescence in the end-plate of enzyme-treated muscles. The postsynaptic localization of the fluorescent probe is therefore tentatively identified as the one producing the cholinolytic effect upon binding to acetylcholine receptor sites."}
{"id": "PMID:1081228", "title": "Binding of autologous erythrocytes to immature T-cells.", "content": "A small percentage of normal mouse thymus and spleen lymphocytes form rosettes with autologous erythrocytes. The number of these autologous rosettes increases 15- to 20-fold after adult thymectomy and to a lesser degree with aging. Autologous rosette level is also abnormally high in nude (congenitally athymic) mice. The high level of autologous rosette-forming cells found after adult thymectomy is normalized by injecting ng amounts of purified circulating thymic factor. Autologous rosette-forming cells adhere to nylon, belong to the less dense spleen cells, are in majority steroid-resistant in the thymus. All these properties suggest that autologous rosette-forming cells might belong to immature T-cell (thymic-dependent cell) precursors.", "contents": "Binding of autologous erythrocytes to immature T-cells. A small percentage of normal mouse thymus and spleen lymphocytes form rosettes with autologous erythrocytes. The number of these autologous rosettes increases 15- to 20-fold after adult thymectomy and to a lesser degree with aging. Autologous rosette level is also abnormally high in nude (congenitally athymic) mice. The high level of autologous rosette-forming cells found after adult thymectomy is normalized by injecting ng amounts of purified circulating thymic factor. Autologous rosette-forming cells adhere to nylon, belong to the less dense spleen cells, are in majority steroid-resistant in the thymus. All these properties suggest that autologous rosette-forming cells might belong to immature T-cell (thymic-dependent cell) precursors."}
{"id": "PMID:1081229", "title": "Idiotype positive T cells visualized by autoradiography and electron microscopy.", "content": "Anti-idiotypic antibodies were prepared in (CBA X C57BL/6)F1 hybrid mice by immunization either with CBA anti-C57BL/6 alloantiserum or with purified CBA thymus-processed lymphoid cells (T cells) Iodinated anti-mouse Ig or triple sandwich ferritin-labeling techniques served to visualize the reaction between idiotype and anti-idiotype. From 5 to 10% of purified CBA T cells appeared to carry receptors for C57BL/6 antigens. Heavily labeled cells had the morphology of small lymphocytes.", "contents": "Idiotype positive T cells visualized by autoradiography and electron microscopy. Anti-idiotypic antibodies were prepared in (CBA X C57BL/6)F1 hybrid mice by immunization either with CBA anti-C57BL/6 alloantiserum or with purified CBA thymus-processed lymphoid cells (T cells) Iodinated anti-mouse Ig or triple sandwich ferritin-labeling techniques served to visualize the reaction between idiotype and anti-idiotype. From 5 to 10% of purified CBA T cells appeared to carry receptors for C57BL/6 antigens. Heavily labeled cells had the morphology of small lymphocytes."}
{"id": "PMID:1081230", "title": "Acetylcholine receptors at neuromuscular synapses: phylogenetic differences detected by snake alpha-neurotoxins.", "content": "Phylogenetic differences in acetylcholine receptors from skeletal neuromuscular synapses of various species of snakes and lizards have been investigated, using the snake venom alpha-neurotoxins alpha-atratoxin (cobrotoxin) and alpha-bungarotoxin. The acetylcholine receptors of the phylogenetically primitive lizards, like those from all other vertebrates previously tested, are blocked by these alpha-neurotoxins. In contrast, receptors from snakes and advanced lizards are insensitive to one or both of the toxins. It is suggested that toxin-resistant acetylcholine receptors appeared early in the evolution of Squamata and preceded the appearance of alpha-neurotoxins.", "contents": "Acetylcholine receptors at neuromuscular synapses: phylogenetic differences detected by snake alpha-neurotoxins. Phylogenetic differences in acetylcholine receptors from skeletal neuromuscular synapses of various species of snakes and lizards have been investigated, using the snake venom alpha-neurotoxins alpha-atratoxin (cobrotoxin) and alpha-bungarotoxin. The acetylcholine receptors of the phylogenetically primitive lizards, like those from all other vertebrates previously tested, are blocked by these alpha-neurotoxins. In contrast, receptors from snakes and advanced lizards are insensitive to one or both of the toxins. It is suggested that toxin-resistant acetylcholine receptors appeared early in the evolution of Squamata and preceded the appearance of alpha-neurotoxins."}
{"id": "PMID:1081231", "title": "Primary acceptor in bacterial photosynthesis: obligatory role of ubiquinone in photoactive reaction centers of Rhodopseudomonas spheroides.", "content": "Reaction centers were found to bind two ubiquinones, both of which could be removed by o-phenanthroline and the detergent lauryldimethylamine oxide. One ubiquinone was more easily removed  than the other. The low-temperature light-induced optical and electron paramagnetic resonance (EPR) changes were eliminated and restored upon removal and readdition of ubiquinone and were quantitatively correlated with the amount of tightly bound ubiquinone. We, therefore, conclude that this ubiquinone plays an obligatory role in the primary photochemistry. The easily removed ubiquinone is thought to be the secondary electron acceptor. The low-temperature charge recombination kinetics, as well as the optical and EPR spectra, were the same for untreated reaction centers and for those reconstituted with ubiquinone. This indicates that extraction and reconstitution were accomplished without altering the conformation of the active site. Reaction centers reconstituted with other quinones also showed restored photochemical activity, although they exhibited changes in their low-temperature recombination kinetics and light-induced (g = 1.8) EPR signal is interpreted in terms of a magnetically coupled ubiquinone--Fe2+ acceptor complex. A possible role of iron is to facilitate electron transfer between the primary and secondary ubiquinones.", "contents": "Primary acceptor in bacterial photosynthesis: obligatory role of ubiquinone in photoactive reaction centers of Rhodopseudomonas spheroides. Reaction centers were found to bind two ubiquinones, both of which could be removed by o-phenanthroline and the detergent lauryldimethylamine oxide. One ubiquinone was more easily removed  than the other. The low-temperature light-induced optical and electron paramagnetic resonance (EPR) changes were eliminated and restored upon removal and readdition of ubiquinone and were quantitatively correlated with the amount of tightly bound ubiquinone. We, therefore, conclude that this ubiquinone plays an obligatory role in the primary photochemistry. The easily removed ubiquinone is thought to be the secondary electron acceptor. The low-temperature charge recombination kinetics, as well as the optical and EPR spectra, were the same for untreated reaction centers and for those reconstituted with ubiquinone. This indicates that extraction and reconstitution were accomplished without altering the conformation of the active site. Reaction centers reconstituted with other quinones also showed restored photochemical activity, although they exhibited changes in their low-temperature recombination kinetics and light-induced (g = 1.8) EPR signal is interpreted in terms of a magnetically coupled ubiquinone--Fe2+ acceptor complex. A possible role of iron is to facilitate electron transfer between the primary and secondary ubiquinones."}
{"id": "PMID:1081233", "title": "Isolation and identification of alpha1-antitrypsin as a component of normal and malignant human breast and other tissues.", "content": "Alpha1-Antitrypsin has been detected using radial immunodiffusion in the 90,000g supernatant fraction of malignant and adjacent normal human breast, colon, and anal tissue, as well as malignant lung, stomach, and ileum. Immobilized chymotrypsin, bound to Sepharose or Affi-Gel 10 has been utilized to separate 11 peaks of antiproteolytic activity by affinity chromatography of normal and malignant human breast tissue extracts. Glycoproteins are associated with eight of the peaks. Peak 1 contains predominantly alpha1-antitrypsin in addition to a minor component. The purification of the inhibitors, as judged by disc gel electrophoresis, is extensive. In some peaks, only one or two protein bands are observed, suggesting that affinity chromatography on Sepharose- or Affi-Gel 10-chymotrypsin might be used for the isolation of alpha1-antitrypsin and other inhibitors in preparative amounts.", "contents": "Isolation and identification of alpha1-antitrypsin as a component of normal and malignant human breast and other tissues. Alpha1-Antitrypsin has been detected using radial immunodiffusion in the 90,000g supernatant fraction of malignant and adjacent normal human breast, colon, and anal tissue, as well as malignant lung, stomach, and ileum. Immobilized chymotrypsin, bound to Sepharose or Affi-Gel 10 has been utilized to separate 11 peaks of antiproteolytic activity by affinity chromatography of normal and malignant human breast tissue extracts. Glycoproteins are associated with eight of the peaks. Peak 1 contains predominantly alpha1-antitrypsin in addition to a minor component. The purification of the inhibitors, as judged by disc gel electrophoresis, is extensive. In some peaks, only one or two protein bands are observed, suggesting that affinity chromatography on Sepharose- or Affi-Gel 10-chymotrypsin might be used for the isolation of alpha1-antitrypsin and other inhibitors in preparative amounts."}
{"id": "PMID:1081235", "title": "Length-dependent optical diffraction pattern changes in frog sartorius muscle.", "content": "The length dependence of the laser light diffraction pattern produced by R. pipiens whole sartorius muscle has been examined at rest and during tetanic contraction. The muscle diffraction pattern was scanned by a vidicon camera; camera output was digitized and processed by an on-line digital computer to allow a real line display of a section through the diffraction pattern. Analysis of the first order diffraction line profiles yielded values for line amplitude, intensity, center of gravity, line width and percent dispersion. The calculated dispersion provided a measure of the muscle's sarcomere length distribution. First order line amplitudes and intensities were observed to increase, then decrease, with progressive stretch from 1.0 to 1.3 reference length. The percent dispersion among sarcomeres in resting muscle averaged 11% and was approximately proportional (coefficient = .0341) to length. The amplitude and intensity of zero and first order diffraction lines decreased during tetanus, while the line widths and dispersions increased. First order line intesity during tetanus was maximum at about the same length as during rest, i.e., at approximately 2.5 mu sarcomere length. Sarcomere dispersion increased by about 6% during tetanus.", "contents": "Length-dependent optical diffraction pattern changes in frog sartorius muscle. The length dependence of the laser light diffraction pattern produced by R. pipiens whole sartorius muscle has been examined at rest and during tetanic contraction. The muscle diffraction pattern was scanned by a vidicon camera; camera output was digitized and processed by an on-line digital computer to allow a real line display of a section through the diffraction pattern. Analysis of the first order diffraction line profiles yielded values for line amplitude, intensity, center of gravity, line width and percent dispersion. The calculated dispersion provided a measure of the muscle's sarcomere length distribution. First order line amplitudes and intensities were observed to increase, then decrease, with progressive stretch from 1.0 to 1.3 reference length. The percent dispersion among sarcomeres in resting muscle averaged 11% and was approximately proportional (coefficient = .0341) to length. The amplitude and intensity of zero and first order diffraction lines decreased during tetanus, while the line widths and dispersions increased. First order line intesity during tetanus was maximum at about the same length as during rest, i.e., at approximately 2.5 mu sarcomere length. Sarcomere dispersion increased by about 6% during tetanus."}
{"id": "PMID:1081237", "title": "Long-term prognosis of idiopathic gastric erosions.", "content": "The long-term prognosis of multiple gastric erosions (erosive gastritis) is analyzed in 58 cases observed over 10-17 years, showing the disease to recur through decades. Idiopathic erosions occur mainly in the antrum and can be visualized roentgenologically, whereas \"stress\" erosions are in the body of the stomach and may only be diagnosed endoscopically.", "contents": "Long-term prognosis of idiopathic gastric erosions. The long-term prognosis of multiple gastric erosions (erosive gastritis) is analyzed in 58 cases observed over 10-17 years, showing the disease to recur through decades. Idiopathic erosions occur mainly in the antrum and can be visualized roentgenologically, whereas \"stress\" erosions are in the body of the stomach and may only be diagnosed endoscopically."}
{"id": "PMID:1081238", "title": "The effect of barium on blood in the gastrointestinal tract.", "content": "The effect of various commercial barium sulfate preparations on blood coagulation, flocculation, and hemolysis was studied. There was considerable variation not only among different barium products but also among different concentrations of the same product. There is no good experimental evidence that barium enemas do stop bleeding. It is probably best to avoid the use of any enema in attempting to control major colonic bleeding; but if an enema is given for this purpose, a tap-water enema is better, as it contains no anticoagulants and is more effective in producing clot formation than most barium suspensions.", "contents": "The effect of barium on blood in the gastrointestinal tract. The effect of various commercial barium sulfate preparations on blood coagulation, flocculation, and hemolysis was studied. There was considerable variation not only among different barium products but also among different concentrations of the same product. There is no good experimental evidence that barium enemas do stop bleeding. It is probably best to avoid the use of any enema in attempting to control major colonic bleeding; but if an enema is given for this purpose, a tap-water enema is better, as it contains no anticoagulants and is more effective in producing clot formation than most barium suspensions."}
{"id": "PMID:1081239", "title": "Hemorrhage from the gastroesophageal junction. A cryptic angiographic diagnosis.", "content": "The angiographic localization of gastroesophageal-junction hemorrage emanating from the left inferior phrenic artery (which originated from the abdominal aorta) is described. The diagnosis was established after conventional selective celiac and left gastric arteriography failed to demonstrate extravasation. The arteriographic evaluation of upper gastrointestinal hemorrhage requires consideration of the variable arterial distribution to the gastroesophageal junction and an awareness of the variable causes of dense opacification (including superimposed adrenal glands) that may mimic extravasation.", "contents": "Hemorrhage from the gastroesophageal junction. A cryptic angiographic diagnosis. The angiographic localization of gastroesophageal-junction hemorrage emanating from the left inferior phrenic artery (which originated from the abdominal aorta) is described. The diagnosis was established after conventional selective celiac and left gastric arteriography failed to demonstrate extravasation. The arteriographic evaluation of upper gastrointestinal hemorrhage requires consideration of the variable arterial distribution to the gastroesophageal junction and an awareness of the variable causes of dense opacification (including superimposed adrenal glands) that may mimic extravasation."}
{"id": "PMID:1081240", "title": "Radiological visualization of the vestibular aqueduct. Technique and morphological features.", "content": "Thin-section tomography in the lateral and off-lateral projections permits radiographic visualization of the vestibular aqueduct, which is seen as an inverted J. The proximal curved portion has a constant configuration, while the distal straight portion varies according to the width of the external aperture and may be tubular, funnel-shaped, or filiform. Nonvisualization of the curved portion does not necessarily mean that it is not present.", "contents": "Radiological visualization of the vestibular aqueduct. Technique and morphological features. Thin-section tomography in the lateral and off-lateral projections permits radiographic visualization of the vestibular aqueduct, which is seen as an inverted J. The proximal curved portion has a constant configuration, while the distal straight portion varies according to the width of the external aperture and may be tubular, funnel-shaped, or filiform. Nonvisualization of the curved portion does not necessarily mean that it is not present."}
{"id": "PMID:1081241", "title": "Electrohydrolytic fragmentation of retained common duct stones.", "content": "The fragmentation of biliary tract stones by the electrohydrolytic principle in vivo is presented. Included is a description of the apparatus.", "contents": "Electrohydrolytic fragmentation of retained common duct stones. The fragmentation of biliary tract stones by the electrohydrolytic principle in vivo is presented. Included is a description of the apparatus."}
{"id": "PMID:1081242", "title": "Surgical treatment of coronary artery disease: pure graft operations, with a study of 741 patients followed 3--7 yr.", "content": "This report reviews the experience with bypass graft surgery in the pure form, without associated cardiac procedures, in 6828 patients operated upon from 1967 through 1974. The hospital mortality rate in this group was 1.4%. The incidence of definite perioperative myocardial infarction was 6.9% prior to 1971, and 4.1% in the past 3 yr. Graft patency in postoperative studies performed an average of over 12 mo after surgery was 83.6%, and 89% of patients had one or more functioning grafts. In a subgroup of 741 consecutive patients operated upon with pure graft techniques from 1967 through 1970, survival seemed to be improved when compared to another group of similar, but nonoperated patients. The average annual mortality rate was 3.3% per yr in the surgical group (including surgical mortality) compared to 8.8% per year in the medical group. Differences in survival were most striking in patients with isolated anterior descending, double and triple vessel involvement. In the 741-patient subgroup the incidence of new occlusions of grafted arteries was related to the severity of the lesion(s) for which the operation was performed, and unrelated to graft patency. Arteriographically demonstrated new occlusions of ungrafted arteries were infrequent, and few patients developed significant new lesions during the period of observation. Symptomatic improvement is related to completeness of revascularization as determined by postoperative arteriography.", "contents": "Surgical treatment of coronary artery disease: pure graft operations, with a study of 741 patients followed 3--7 yr. This report reviews the experience with bypass graft surgery in the pure form, without associated cardiac procedures, in 6828 patients operated upon from 1967 through 1974. The hospital mortality rate in this group was 1.4%. The incidence of definite perioperative myocardial infarction was 6.9% prior to 1971, and 4.1% in the past 3 yr. Graft patency in postoperative studies performed an average of over 12 mo after surgery was 83.6%, and 89% of patients had one or more functioning grafts. In a subgroup of 741 consecutive patients operated upon with pure graft techniques from 1967 through 1970, survival seemed to be improved when compared to another group of similar, but nonoperated patients. The average annual mortality rate was 3.3% per yr in the surgical group (including surgical mortality) compared to 8.8% per year in the medical group. Differences in survival were most striking in patients with isolated anterior descending, double and triple vessel involvement. In the 741-patient subgroup the incidence of new occlusions of grafted arteries was related to the severity of the lesion(s) for which the operation was performed, and unrelated to graft patency. Arteriographically demonstrated new occlusions of ungrafted arteries were infrequent, and few patients developed significant new lesions during the period of observation. Symptomatic improvement is related to completeness of revascularization as determined by postoperative arteriography."}
{"id": "PMID:1081253", "title": "Effects of antiarrhythmic drugs on cardiac membrane conductances; a study using the Hodgkin and Huxley mathematical model.", "content": "A study in voltage clamp conditions of the modifications of the cardiac membrane conductances by quinidine sulfate has been carried out on frog atrial fibers by means of the double sucrose gap technique. The computation of the parameters related to the conductances has been done according to the Hodgkin and Huxley mathematical model proposed in 1952. The computed conductances concern the sodium conductance, the calcium conductance, and the total delayed conductance. A decrease of all of the studied conductances is observed in the presence of quinidine sulfate. This drug also mainly induced a slowing down of several activation, inactivation, and reactivation kinetics. The results obtained allow a more detailed explanation of the mechanism of action of quinidine sulfate in the membrane. Although quinidine is known to possess antiarrhythmic properties, the exact mechanisms of its action are not clear. The present study was therefore undertaken to provide some information on this point.", "contents": "Effects of antiarrhythmic drugs on cardiac membrane conductances; a study using the Hodgkin and Huxley mathematical model. A study in voltage clamp conditions of the modifications of the cardiac membrane conductances by quinidine sulfate has been carried out on frog atrial fibers by means of the double sucrose gap technique. The computation of the parameters related to the conductances has been done according to the Hodgkin and Huxley mathematical model proposed in 1952. The computed conductances concern the sodium conductance, the calcium conductance, and the total delayed conductance. A decrease of all of the studied conductances is observed in the presence of quinidine sulfate. This drug also mainly induced a slowing down of several activation, inactivation, and reactivation kinetics. The results obtained allow a more detailed explanation of the mechanism of action of quinidine sulfate in the membrane. Although quinidine is known to possess antiarrhythmic properties, the exact mechanisms of its action are not clear. The present study was therefore undertaken to provide some information on this point."}
{"id": "PMID:1081254", "title": "Effects of ouabain on sodium uptake by frog heart and skeletal muscle.", "content": "Effects of ouabain (10(-3)-10(-12) M) on frog heart and skeletal muscle net uptake of Na from a K-free Conway-Ringer solution at 0-2 degrees C were investigated. Under these circumstances Na pumps are inactive and fibers are gaining Na even without ouabain. Changes in K, H2O, and solids were also measured. Na gains and K losses were increased by ouabain at greater than 10(-6) and greater than 10(-7) M, respectively, and decreased in heart muscle at less than or equal to 10(-8) M. It is suggested that these effects are due to actions on membrane permeability. Implications for mechanisms of positive inotropic effect of glycosides and for treatment are discussed.", "contents": "Effects of ouabain on sodium uptake by frog heart and skeletal muscle. Effects of ouabain (10(-3)-10(-12) M) on frog heart and skeletal muscle net uptake of Na from a K-free Conway-Ringer solution at 0-2 degrees C were investigated. Under these circumstances Na pumps are inactive and fibers are gaining Na even without ouabain. Changes in K, H2O, and solids were also measured. Na gains and K losses were increased by ouabain at greater than 10(-6) and greater than 10(-7) M, respectively, and decreased in heart muscle at less than or equal to 10(-8) M. It is suggested that these effects are due to actions on membrane permeability. Implications for mechanisms of positive inotropic effect of glycosides and for treatment are discussed."}
{"id": "PMID:1081255", "title": "The action of ethanol upon the action potential and contraction of ventricular muscle.", "content": "Isolated isometric ventricular muscle of frogs and cats was studied. Perfusing solutions were played directly on the muscle to permit rapid exchange of the extracellular space. Developed force and maximal rate of rise of force were measured in all studies and action potentials (AP) were recorded in some. For both species low concentrations of ethanol (75 degrees  mg/l) potentiate contraction. Higher concentrations ( greater than or equal to 750 mg/l) depress contraction progressively with increasing concentration. Concentrations which depress contraction, e.g., 3-4.5 gm/l, usually shorten AP duration. The shortening of AP duration can occur even though contractile force does not fall and, conversely, force may fall while AP duration is unaffected. When 10 mM caffeine is added to the perfusate of either species, AP duration is prolonged and contraction is potentiated. If both ethanol (4.5 gm/l) and 10 mM caffeine are added simultaneously to the perfusate, there is a rapid (within 4 beats) increase in AP duration and an initial depression of contraction, followed by a further increase in AP duration and a significant potentiation of contraction. The steady state contraction is less than with caffeine alone. These preliminary studies suggest that ethanol may depress contraction both by shortening AP duration and by a direct effect upon the contractile apparatus.", "contents": "The action of ethanol upon the action potential and contraction of ventricular muscle. Isolated isometric ventricular muscle of frogs and cats was studied. Perfusing solutions were played directly on the muscle to permit rapid exchange of the extracellular space. Developed force and maximal rate of rise of force were measured in all studies and action potentials (AP) were recorded in some. For both species low concentrations of ethanol (75 degrees  mg/l) potentiate contraction. Higher concentrations ( greater than or equal to 750 mg/l) depress contraction progressively with increasing concentration. Concentrations which depress contraction, e.g., 3-4.5 gm/l, usually shorten AP duration. The shortening of AP duration can occur even though contractile force does not fall and, conversely, force may fall while AP duration is unaffected. When 10 mM caffeine is added to the perfusate of either species, AP duration is prolonged and contraction is potentiated. If both ethanol (4.5 gm/l) and 10 mM caffeine are added simultaneously to the perfusate, there is a rapid (within 4 beats) increase in AP duration and an initial depression of contraction, followed by a further increase in AP duration and a significant potentiation of contraction. The steady state contraction is less than with caffeine alone. These preliminary studies suggest that ethanol may depress contraction both by shortening AP duration and by a direct effect upon the contractile apparatus."}
{"id": "PMID:1081256", "title": "Optical studies on contractile structures during activity.", "content": "The optical properties of myocardial fibers and skeletal fibers were compared at rest and during activity. Scattering by myocardial trabeculae is more important than by skeletal fibers but birefringence of myocardial trabeculae is less important than that of skeletal fibers. The absorbance curves of both isolated myocardial trabeculae and skeletal fibers in vivo reflect their main components; during contraction the absorbance increases mainly at 285 nm and this increase starts earlier than contraction recorded with a mechanotransducer. An absorbance change in the spectral range of 420-440 nm is also reported which could represent very early biochemical change in the contractile fibers. The technique used, combined with electrophysiological data, allows one to study different problems related to the biophysics and biochemistry of contraction.", "contents": "Optical studies on contractile structures during activity. The optical properties of myocardial fibers and skeletal fibers were compared at rest and during activity. Scattering by myocardial trabeculae is more important than by skeletal fibers but birefringence of myocardial trabeculae is less important than that of skeletal fibers. The absorbance curves of both isolated myocardial trabeculae and skeletal fibers in vivo reflect their main components; during contraction the absorbance increases mainly at 285 nm and this increase starts earlier than contraction recorded with a mechanotransducer. An absorbance change in the spectral range of 420-440 nm is also reported which could represent very early biochemical change in the contractile fibers. The technique used, combined with electrophysiological data, allows one to study different problems related to the biophysics and biochemistry of contraction."}
{"id": "PMID:1081271", "title": "The influence of calcium and calcium + vitamin D2 treatment on bone mineral after partial gastrectomy.", "content": "Sixty-three men and 23 women who had undergone partial gastrectomy for ulcer disease were randomized into three groups to study the effect of calcium (2 g per day) and calcium + vitamin D2 (1000 IU calciferol per day) treatment for eight months on bone mineral. The Americium-241 gamma ray attenuation method was used to measure the changes in bone mineral density. Calcium and calcium + vitamin D2 increased the bone mineral density statistically significantly in the males, but not in females. Partial gastrectomy causes bone rarefaction, and consequently these patients reach the risk level of bone mineral density for osteoporotic fractures earlier than healthy subjects. The conclusion drawn was that prophylactic treatment is needed after partial gastrectomy. Further study is required, especially of women, to find the most suitable form of treatment for disturbances in bone mineral metabolism after gastric resection.", "contents": "The influence of calcium and calcium + vitamin D2 treatment on bone mineral after partial gastrectomy. Sixty-three men and 23 women who had undergone partial gastrectomy for ulcer disease were randomized into three groups to study the effect of calcium (2 g per day) and calcium + vitamin D2 (1000 IU calciferol per day) treatment for eight months on bone mineral. The Americium-241 gamma ray attenuation method was used to measure the changes in bone mineral density. Calcium and calcium + vitamin D2 increased the bone mineral density statistically significantly in the males, but not in females. Partial gastrectomy causes bone rarefaction, and consequently these patients reach the risk level of bone mineral density for osteoporotic fractures earlier than healthy subjects. The conclusion drawn was that prophylactic treatment is needed after partial gastrectomy. Further study is required, especially of women, to find the most suitable form of treatment for disturbances in bone mineral metabolism after gastric resection."}
{"id": "PMID:1081272", "title": "Influence of prednisone and cytostatics on human blood B-, T- and O-lymphocytes in diseases.", "content": "B-, T- and  O-lymphocytes detected as EAC-, E- and non-rosette forming lymphocytes were investigated in venous blood in 49 patients with connective tissue diseases, psoriasis and chronic lymphogenous leukaemia (CLL) during treatment with either prednisone alone, prednisone and cytostatic agents or cytostatic agents alone. Prednisone alone did not change the B-, T- and O-lymphocyte counts. Cytostatics alone or in combination with prednisone reduced the B- and T-lymphocyte counts concomitant with a significant increase in the O-lymphocyte count. The findings could be explained by assuming that cytostatics disturb the immunological functions of the lymphocytes and finally deprive the cells of their B- and T-markers. The optimal immunosuppressive treatment with cytostatic agents may be associated with a certain reduction of B- and T-lymphocytes which may be used as a guideline for dosage.", "contents": "Influence of prednisone and cytostatics on human blood B-, T- and O-lymphocytes in diseases. B-, T- and  O-lymphocytes detected as EAC-, E- and non-rosette forming lymphocytes were investigated in venous blood in 49 patients with connective tissue diseases, psoriasis and chronic lymphogenous leukaemia (CLL) during treatment with either prednisone alone, prednisone and cytostatic agents or cytostatic agents alone. Prednisone alone did not change the B-, T- and O-lymphocyte counts. Cytostatics alone or in combination with prednisone reduced the B- and T-lymphocyte counts concomitant with a significant increase in the O-lymphocyte count. The findings could be explained by assuming that cytostatics disturb the immunological functions of the lymphocytes and finally deprive the cells of their B- and T-markers. The optimal immunosuppressive treatment with cytostatic agents may be associated with a certain reduction of B- and T-lymphocytes which may be used as a guideline for dosage."}
{"id": "PMID:1081273", "title": "A study of proteolysis as a possible mechanism for T-cell-mediated target cell lysis.", "content": "The hypothesis that proteolysis is implicated in T-cell-mediated target cell lysis was tested in two ways: first, various inhibitors of protease activity were used either during or before the cytotoxicity test. Ovomucoid, TPCK, and PMSF proved to be not or only marginally inhibitory, whereas the considerable inhibitory action of TLCK was shown to most likely be a toxic effect of the inhibitor. Second, radioactive substrates for cathepsin activity were used, either free in the medium or fixed on the surface of the target cells, to detect a possible breakdown of these substrates by a release of intracellular cathepsins during the cytotoxicity test. Very little dialysable breakdown products were formed, and there was no difference between their amount in either a homologus or a heterologous system. It is concluded that proteolysis probably is not the mechanism by which target cells are killed. Alternative mechanisms are discussed.", "contents": "A study of proteolysis as a possible mechanism for T-cell-mediated target cell lysis. The hypothesis that proteolysis is implicated in T-cell-mediated target cell lysis was tested in two ways: first, various inhibitors of protease activity were used either during or before the cytotoxicity test. Ovomucoid, TPCK, and PMSF proved to be not or only marginally inhibitory, whereas the considerable inhibitory action of TLCK was shown to most likely be a toxic effect of the inhibitor. Second, radioactive substrates for cathepsin activity were used, either free in the medium or fixed on the surface of the target cells, to detect a possible breakdown of these substrates by a release of intracellular cathepsins during the cytotoxicity test. Very little dialysable breakdown products were formed, and there was no difference between their amount in either a homologus or a heterologous system. It is concluded that proteolysis probably is not the mechanism by which target cells are killed. Alternative mechanisms are discussed."}
{"id": "PMID:1081275", "title": "Thymus: central role in the immune system of the frog.", "content": "In the leopard frog (Rana pipiens), the thymus profoundly influences immunologic development by providing virtually all the progenitor lymphocytic cells for the bone marrow and other peripheral lymphoid organs. The thymus houses a self-perpetuating population of lymphopoietic cells that originally self-differentiated in that gland.", "contents": "Thymus: central role in the immune system of the frog. In the leopard frog (Rana pipiens), the thymus profoundly influences immunologic development by providing virtually all the progenitor lymphocytic cells for the bone marrow and other peripheral lymphoid organs. The thymus houses a self-perpetuating population of lymphopoietic cells that originally self-differentiated in that gland."}
{"id": "PMID:1081278", "title": "Role of coronary artery surgery in patients surviving unexpected cardiac arrest.", "content": "During the past 3-1/2 years, 101 of 199 patients who were successfully resuscitated by fire rescue squads in the community after unexpected cardiac arrest (documented ventricular fibrillation--VF) were admitted to the hospital. Forty-two of these patients ultimately were discharged from the hospital. However, the intermediate and long-term results were disappointing, for their mean survival after discharge was only 12.7 months. Sudden deaths (recurrent unexpected VF) occurred during the first 12 months after discharge in 28 percent of the patients surviving the initial hospitalization. Among the survivors of sudden and unexpected VF (i.e., survivors of the initial hospitalization), 16 patients who had had pre-existing symptoms of coronary heart disease had hemodynamic and coronary angiographic studies. Of these, 11 were considered surgical candidates. This report concerns the follow-up results in these patients with particular emphasis on the eight patients who accepted surgery and had myocardial revascularization. Five patients had moderate hemodynamic abnormalities, but none had had an acute myocardial infarction at the time of the initial arrest. The group of eight patients had a total of 18 vein graft bypasses performed. The significant findings is that all eight survived operation, and there was one late death at 10 months. The remaining patients are alive at 14 to 34 months, and six are free of symptoms. Despite patent vein grafts, one patient has had a second serious arrhythmia. It is concluded that surgical intervention can be done safely and may decrease the high posthospitalization, recurrent arrest, and mortality rates in selected survivors of unexpected cardiac arrest. It is concluded further that all patients sustaining an unexpected arrest should have postarrest coronary catheterization and angiography, and all patients should be on antiarrhythmic agents whether or not they receive myocardial revascularization.", "contents": "Role of coronary artery surgery in patients surviving unexpected cardiac arrest. During the past 3-1/2 years, 101 of 199 patients who were successfully resuscitated by fire rescue squads in the community after unexpected cardiac arrest (documented ventricular fibrillation--VF) were admitted to the hospital. Forty-two of these patients ultimately were discharged from the hospital. However, the intermediate and long-term results were disappointing, for their mean survival after discharge was only 12.7 months. Sudden deaths (recurrent unexpected VF) occurred during the first 12 months after discharge in 28 percent of the patients surviving the initial hospitalization. Among the survivors of sudden and unexpected VF (i.e., survivors of the initial hospitalization), 16 patients who had had pre-existing symptoms of coronary heart disease had hemodynamic and coronary angiographic studies. Of these, 11 were considered surgical candidates. This report concerns the follow-up results in these patients with particular emphasis on the eight patients who accepted surgery and had myocardial revascularization. Five patients had moderate hemodynamic abnormalities, but none had had an acute myocardial infarction at the time of the initial arrest. The group of eight patients had a total of 18 vein graft bypasses performed. The significant findings is that all eight survived operation, and there was one late death at 10 months. The remaining patients are alive at 14 to 34 months, and six are free of symptoms. Despite patent vein grafts, one patient has had a second serious arrhythmia. It is concluded that surgical intervention can be done safely and may decrease the high posthospitalization, recurrent arrest, and mortality rates in selected survivors of unexpected cardiac arrest. It is concluded further that all patients sustaining an unexpected arrest should have postarrest coronary catheterization and angiography, and all patients should be on antiarrhythmic agents whether or not they receive myocardial revascularization."}
{"id": "PMID:1081284", "title": "Antibody-dependent and direct lymphocyte-mediated cytotoxicity in patients on hemodialysis.", "content": "Twenty-two patients on chronic hemodialysis were tested for direct lymphocyte-mediated cytotoxicity (DCML) and antibody-dependent lymphocyte-mediated cytotoxicity (LALI) against selected panels of normal donor lymphocytes. The DCML reaction was only positive in patients who had received blood transfusions within the two or three months prior to testing. Thus this reaction has limited value in practice as a parameter for presensitization. The use of third-party effector cells and of target cells precoated with antibody in the LALI reaction was evaluated, and it is advocated that effector cells from the patient investigated and direct application of patient serum to reaction mixtures should be preferred in order to avoid false negative reactions. In patients who had once manifested lymphocytotoxins, LALI disclosed a much broader reactivity than seen with a conventional complement-dependent cytotoxicity technique (CDC), whereas in this study patients who had never developed cytotoxins were also negative with the LALI technique. It is concluded that a positive LALI cross-match, in spite of a negative CDC cross-match, will be a common finding in donor-recipient combinations where the recipient has preformed cytotoxins. The effect of this on subsequent graft prognosis needs further clarification.", "contents": "Antibody-dependent and direct lymphocyte-mediated cytotoxicity in patients on hemodialysis. Twenty-two patients on chronic hemodialysis were tested for direct lymphocyte-mediated cytotoxicity (DCML) and antibody-dependent lymphocyte-mediated cytotoxicity (LALI) against selected panels of normal donor lymphocytes. The DCML reaction was only positive in patients who had received blood transfusions within the two or three months prior to testing. Thus this reaction has limited value in practice as a parameter for presensitization. The use of third-party effector cells and of target cells precoated with antibody in the LALI reaction was evaluated, and it is advocated that effector cells from the patient investigated and direct application of patient serum to reaction mixtures should be preferred in order to avoid false negative reactions. In patients who had once manifested lymphocytotoxins, LALI disclosed a much broader reactivity than seen with a conventional complement-dependent cytotoxicity technique (CDC), whereas in this study patients who had never developed cytotoxins were also negative with the LALI technique. It is concluded that a positive LALI cross-match, in spite of a negative CDC cross-match, will be a common finding in donor-recipient combinations where the recipient has preformed cytotoxins. The effect of this on subsequent graft prognosis needs further clarification."}
{"id": "PMID:1081285", "title": "Low serum alpha-1-antitrypsin level as a contributory factor of combined emphysema in silicosis.", "content": "In order to evaluate low serum alpha-1-antitrypsin level as a contributory factor of combined emphysema in silicotic patients, serum alpha-1-antitrypsin analysis was carried out in 80 patients with silicosis. Low serum alpha-1-antitrypsin level was found in 5 patients. Large opacities were observed roentgenologically in only 1 out of these 5 cases in contrast to 31 of the other 75 cases. Also the suggestive findings for the emphysema were showed in 4 of these 5 cases while such findings were found on their chest X-ray films in only 43 of the other 75 cases. FEV 1.0% below 50 were calculated in 3 of the 5 cases, on the contrary in 22 of the 75 cases. RV above 50% predicted value was showed by all the 5 low antitrypsin patients, in contrast to only 25 of other 74 cases. The silicotic patients with low serum alpha-1-antitrypsin concentration are most likely to have an association with a high incidence of complicated emphysema.", "contents": "Low serum alpha-1-antitrypsin level as a contributory factor of combined emphysema in silicosis. In order to evaluate low serum alpha-1-antitrypsin level as a contributory factor of combined emphysema in silicotic patients, serum alpha-1-antitrypsin analysis was carried out in 80 patients with silicosis. Low serum alpha-1-antitrypsin level was found in 5 patients. Large opacities were observed roentgenologically in only 1 out of these 5 cases in contrast to 31 of the other 75 cases. Also the suggestive findings for the emphysema were showed in 4 of these 5 cases while such findings were found on their chest X-ray films in only 43 of the other 75 cases. FEV 1.0% below 50 were calculated in 3 of the 5 cases, on the contrary in 22 of the 75 cases. RV above 50% predicted value was showed by all the 5 low antitrypsin patients, in contrast to only 25 of other 74 cases. The silicotic patients with low serum alpha-1-antitrypsin concentration are most likely to have an association with a high incidence of complicated emphysema."}
{"id": "PMID:1081286", "title": "The monoaminergic innervation of the telencephalon of the frog, Rana pipiens.", "content": "A large number of serotonin (5-hydroxytryptamine, 5-HT)-type axon terminals has been visualized in the so-called 'striatum' (pars ventralis and dorsalis) of Rana pipiens by means of the Falck-Hillarp histochemical method. The frog striatum contains, however, a much smaller number of catecholamine (CA) type axon terminals and differs strikingly in that aspect from the neostriatum of 'higher' vertebrates which is known to receive a massive CA innervation. In the telencephalon of Rana pipiens the highest density of CA axon terminals occurs at the level of the medial wall, which appears highly hypertrophied in comparison to the thin lateral wall. The CA terminals are mainly concentrated within nucleus accumbens septi and in the ventrolateral portion of nucleus lateralis septi where they surround intimately the non-fluorescent neuronal somata of both septal nuclei. The three pallial fields (medial, dorsal and lateral) of Rana pipiens are nearly devoid of CA axon terminals. A moderate amount of 5-HT terminals is present, however, within the caudal half of both the medial and dorsal pallial fields. Finally, a few CA-type neuronal somata are found lying amongst the mitral cells of the olfactory bulb. As a whole, the telecenphalon of Rana pipiens appears more profusely innervated by axons of the 5-HT-type than by those of the CA-type. This finding has been related to the fact that in the frog brain stem the CA neuronal somata are scarce in comparison to the highly developed 5-HT neuronal systems.", "contents": "The monoaminergic innervation of the telencephalon of the frog, Rana pipiens. A large number of serotonin (5-hydroxytryptamine, 5-HT)-type axon terminals has been visualized in the so-called 'striatum' (pars ventralis and dorsalis) of Rana pipiens by means of the Falck-Hillarp histochemical method. The frog striatum contains, however, a much smaller number of catecholamine (CA) type axon terminals and differs strikingly in that aspect from the neostriatum of 'higher' vertebrates which is known to receive a massive CA innervation. In the telencephalon of Rana pipiens the highest density of CA axon terminals occurs at the level of the medial wall, which appears highly hypertrophied in comparison to the thin lateral wall. The CA terminals are mainly concentrated within nucleus accumbens septi and in the ventrolateral portion of nucleus lateralis septi where they surround intimately the non-fluorescent neuronal somata of both septal nuclei. The three pallial fields (medial, dorsal and lateral) of Rana pipiens are nearly devoid of CA axon terminals. A moderate amount of 5-HT terminals is present, however, within the caudal half of both the medial and dorsal pallial fields. Finally, a few CA-type neuronal somata are found lying amongst the mitral cells of the olfactory bulb. As a whole, the telecenphalon of Rana pipiens appears more profusely innervated by axons of the 5-HT-type than by those of the CA-type. This finding has been related to the fact that in the frog brain stem the CA neuronal somata are scarce in comparison to the highly developed 5-HT neuronal systems."}
{"id": "PMID:1081287", "title": "Evidence that monoamines influence human evoked potentials.", "content": "We have measured latency and flash/pattern differentials of the visual evoked potential (VEP) from phenylketonuric humans, while systematically manipulating rates of amine synthesis in the central nervous system using two techniques. We have observed that stimulation of monoaminergic activity in the visual processing system, either by lowering inhibitory levels of phenylalanine through dietary restriction or by a properly balanced administration of indole and catecholamine precursors, shortened VEP latencies and permitted the development of a discriminative brain response to patterned stimuli. The close temporal relationship between these electrophysiological changes and the neurochemical manipulations following treatment initiation of discontinuation argue that monoamines play a significant role in the mediation of human sensory evoked potentials.", "contents": "Evidence that monoamines influence human evoked potentials. We have measured latency and flash/pattern differentials of the visual evoked potential (VEP) from phenylketonuric humans, while systematically manipulating rates of amine synthesis in the central nervous system using two techniques. We have observed that stimulation of monoaminergic activity in the visual processing system, either by lowering inhibitory levels of phenylalanine through dietary restriction or by a properly balanced administration of indole and catecholamine precursors, shortened VEP latencies and permitted the development of a discriminative brain response to patterned stimuli. The close temporal relationship between these electrophysiological changes and the neurochemical manipulations following treatment initiation of discontinuation argue that monoamines play a significant role in the mediation of human sensory evoked potentials."}
{"id": "PMID:1081288", "title": "[Demonstration of gastrointestinal hemorrhages in the acute radiation syndrome].", "content": "Increased radioiron excretion with feces following irradiation is a sure indicator of thrombopenia-induced gastrointestinal hemorrhages which are to be regarded as one of the main causes of the acute radiation syndrome or radiation death respectively. We analyzed the daily 59Fe- excretion of whole-body irradiated mice from the 7th to the 16th day after radiation insult, this period being the critical phase in the course of acute radiopathy with a mortality amounting here to about 50% of the irradiated animals (bone-marrow syndrome). On the eleventh day after radiation exposure, 59Fe-excretion of irradiated animals significantly exceeded by more than fivefold that of the controls. If, before radiation exposure, the mice have been treated with the protective agent AET, they survived without exception and, moreover, a significantly reduced 59Fe-excretion was noted too, the control values almost being reached again. By means of the radionuclide 59Fe therefore, ont only statements are possible concerning the functional state of the erythropoietic system, but also troubles of the hemostatic system can be detected, e.g. the pathological transition of blood into the gastrointestinal tract.", "contents": "[Demonstration of gastrointestinal hemorrhages in the acute radiation syndrome]. Increased radioiron excretion with feces following irradiation is a sure indicator of thrombopenia-induced gastrointestinal hemorrhages which are to be regarded as one of the main causes of the acute radiation syndrome or radiation death respectively. We analyzed the daily 59Fe- excretion of whole-body irradiated mice from the 7th to the 16th day after radiation insult, this period being the critical phase in the course of acute radiopathy with a mortality amounting here to about 50% of the irradiated animals (bone-marrow syndrome). On the eleventh day after radiation exposure, 59Fe-excretion of irradiated animals significantly exceeded by more than fivefold that of the controls. If, before radiation exposure, the mice have been treated with the protective agent AET, they survived without exception and, moreover, a significantly reduced 59Fe-excretion was noted too, the control values almost being reached again. By means of the radionuclide 59Fe therefore, ont only statements are possible concerning the functional state of the erythropoietic system, but also troubles of the hemostatic system can be detected, e.g. the pathological transition of blood into the gastrointestinal tract."}
{"id": "PMID:1081289", "title": "[Liquor-scintigraphic findings with Yb 169-DTPA in cerebro-atrophic processes].", "content": "Liquor-scintigraphic examinations were carried out with Yb 169-DTPA in two patients, who were offering - clinically as well as by pneumo-encephalographic findings - the picture of a cerebro-atrophic process. Especially the first case showed the typical course and symptoms of Alzheimer's disease. A \"stasis\" of the radionuclide, lasting for 48 hours, was manifesting liquor-scintigraphically herein, in the course of which there was - initially - a weak representation of the ventricles. However, the complete clinical picture of a communicating hydrocephalus did not show up. In the second case, an undisturbed liquor circulation and absorption, to a great extent topically and temporally undisturbed, were observed. The question arises whether Alzheimer's disease is manifesting together with such a disturbance of the liquor circulation, already described also by other workers. The second case makes clear that the liquor space scintigraphy cannot be used as only diagnostic method in all cerebro-atrophic processes.", "contents": "[Liquor-scintigraphic findings with Yb 169-DTPA in cerebro-atrophic processes]. Liquor-scintigraphic examinations were carried out with Yb 169-DTPA in two patients, who were offering - clinically as well as by pneumo-encephalographic findings - the picture of a cerebro-atrophic process. Especially the first case showed the typical course and symptoms of Alzheimer's disease. A \"stasis\" of the radionuclide, lasting for 48 hours, was manifesting liquor-scintigraphically herein, in the course of which there was - initially - a weak representation of the ventricles. However, the complete clinical picture of a communicating hydrocephalus did not show up. In the second case, an undisturbed liquor circulation and absorption, to a great extent topically and temporally undisturbed, were observed. The question arises whether Alzheimer's disease is manifesting together with such a disturbance of the liquor circulation, already described also by other workers. The second case makes clear that the liquor space scintigraphy cannot be used as only diagnostic method in all cerebro-atrophic processes."}
{"id": "PMID:1081290", "title": "Maternal mortality in Western Nigeria.", "content": "Data on maternal deaths were collected from a randomly selected sample of medical institutions in the Western State of Nigeria for the years 1972 and 1973. An overall maternal mortality rate of 3.8/1000 total births were recorded for 1972 and 1973 respectively. The major causes of maternal mortality were haemorrhage, obstructed labour, eclampsia, anaemia of pregnancy and infection.", "contents": "Maternal mortality in Western Nigeria. Data on maternal deaths were collected from a randomly selected sample of medical institutions in the Western State of Nigeria for the years 1972 and 1973. An overall maternal mortality rate of 3.8/1000 total births were recorded for 1972 and 1973 respectively. The major causes of maternal mortality were haemorrhage, obstructed labour, eclampsia, anaemia of pregnancy and infection."}
{"id": "PMID:1081291", "title": "[Substitution of T-cell function by poly-4-vinypyridine in the formation of the primary and secondary immune response].", "content": "The influence of poly-4-vinylpiridine (P-4-VP) on the production of antibody forming cells (AFC) has been studied. P-4-VP induces helper activity in lymphoid organs of B-mice. P-4-VP injected into mice substitutes T cell function in the development of immunological memory. The possibility of substitution by P-4-VP of various functions or of different subpopulations of T cells in T cell-deprived mice is discussed.", "contents": "[Substitution of T-cell function by poly-4-vinypyridine in the formation of the primary and secondary immune response]. The influence of poly-4-vinylpiridine (P-4-VP) on the production of antibody forming cells (AFC) has been studied. P-4-VP induces helper activity in lymphoid organs of B-mice. P-4-VP injected into mice substitutes T cell function in the development of immunological memory. The possibility of substitution by P-4-VP of various functions or of different subpopulations of T cells in T cell-deprived mice is discussed."}
{"id": "PMID:1081293", "title": "[Bone marrow reaction to surgical intervention in gastroduodenal hemorrhage].", "content": "Experimentally and clinically, the authors have studied the effect of massive blood loss and various operative procedures (Billroth I and Hofmeister-Finsterer gastrectomies, pyloroplasty with vagotomy) on the hemopietic system. It has been found that the bone marrow response was mainly dependent on blood loss. A surgical intervention renders but insignificant effect on hemopoiesis, similar to that of a moderate hemorrhage, the character of an operative procedure being of no improtance.", "contents": "[Bone marrow reaction to surgical intervention in gastroduodenal hemorrhage]. Experimentally and clinically, the authors have studied the effect of massive blood loss and various operative procedures (Billroth I and Hofmeister-Finsterer gastrectomies, pyloroplasty with vagotomy) on the hemopietic system. It has been found that the bone marrow response was mainly dependent on blood loss. A surgical intervention renders but insignificant effect on hemopoiesis, similar to that of a moderate hemorrhage, the character of an operative procedure being of no improtance."}
{"id": "PMID:1081294", "title": "[Characteristics of blood coagulation and rational hemostatic therapy of acute gastroduodenal hemorrhage].", "content": "The results of studies on the state of the coagulative and fibrinolytic system of blood in 78 patients with acute gastroduodenal hemorrhages are summarized. The causes of bleeding were as follows: ulcerous gastroduodenal disease -- in 38 cases, cancer of the stomach -- in 20, esophageal venous varices -- in 11 and erosive gastritis -- in 9. Some features of hemorrhage due to erosive gastritis, esophageal varices, ulcerous disease and gastric cancer are described.", "contents": "[Characteristics of blood coagulation and rational hemostatic therapy of acute gastroduodenal hemorrhage]. The results of studies on the state of the coagulative and fibrinolytic system of blood in 78 patients with acute gastroduodenal hemorrhages are summarized. The causes of bleeding were as follows: ulcerous gastroduodenal disease -- in 38 cases, cancer of the stomach -- in 20, esophageal venous varices -- in 11 and erosive gastritis -- in 9. Some features of hemorrhage due to erosive gastritis, esophageal varices, ulcerous disease and gastric cancer are described."}
{"id": "PMID:1081310", "title": "[Gastroscopy -- a routine procedure in the investigation of diseases of the stomach (author's transl)].", "content": "After a short review of the development of endoscopic investigations, the technique of gastroscopy is described and the indications for this procedure are pointed out. This procedure is of equal importance as radio-diagnostic measures in the routine investigation of stomach diseases and is of particular value when used as an emergency procedure and in the follow-up of patients after gastric operations. It also offers great advantages in the diagnosis of stomach tumours since biopsy material can be obtained from the lesion. Stomach haemorrhage due to gastric erosion, oesophagitis and the Mallory-Weiss syndrome are best diagnosed by gastroscopy. There are practically no contraindications to gastroscopy. The only precluding factor being an uncooperative patient. It is not contraindicated in patients with oesophageal varices or deformity of the spinal column. Complications are very rare. In the case of emergency gastrocscopy, shock must be counteracted and the blood pressure normalized before the investigation can be performed.", "contents": "[Gastroscopy -- a routine procedure in the investigation of diseases of the stomach (author's transl)]. After a short review of the development of endoscopic investigations, the technique of gastroscopy is described and the indications for this procedure are pointed out. This procedure is of equal importance as radio-diagnostic measures in the routine investigation of stomach diseases and is of particular value when used as an emergency procedure and in the follow-up of patients after gastric operations. It also offers great advantages in the diagnosis of stomach tumours since biopsy material can be obtained from the lesion. Stomach haemorrhage due to gastric erosion, oesophagitis and the Mallory-Weiss syndrome are best diagnosed by gastroscopy. There are practically no contraindications to gastroscopy. The only precluding factor being an uncooperative patient. It is not contraindicated in patients with oesophageal varices or deformity of the spinal column. Complications are very rare. In the case of emergency gastrocscopy, shock must be counteracted and the blood pressure normalized before the investigation can be performed."}
{"id": "PMID:1081311", "title": "[Algesimetric measurement of the effect of cerebral electrotherapy on the pain threshold (author's transl)].", "content": "The effect of cerebral electrotherapy (CET) on the pain threshold was investigated in 3 test persons, on 5 points of the neck of these persons. Altogether 1200 algesimetric measurements were performed. The statistical evaluation showed a significant tedency (p less than 0.01) towards an elevation of the pain threshold by CET.", "contents": "[Algesimetric measurement of the effect of cerebral electrotherapy on the pain threshold (author's transl)]. The effect of cerebral electrotherapy (CET) on the pain threshold was investigated in 3 test persons, on 5 points of the neck of these persons. Altogether 1200 algesimetric measurements were performed. The statistical evaluation showed a significant tedency (p less than 0.01) towards an elevation of the pain threshold by CET."}
{"id": "PMID:1081314", "title": "[Unusual generalized granulomatosis. Two case reports (author's transl)].", "content": "Report about two cases of unusual generalized granulomatosis in males, with multiple lymphomas and involvement of internal organs and brain, leading soon to death. The diagnosis was malignant histiocytosis and disseminated eosinophilic granulomatosis. The malignant histiocytosis is differentiated from malignant fibroxantoma (malignant histiocytoma). The unusual cerebral features in the second case - diagnosed as disseminated eosinophilic granulomatosis - seem to show some relations to histiocytosis X. The problems in diagnosis of generalized granulomatosis are pointed out.", "contents": "[Unusual generalized granulomatosis. Two case reports (author's transl)]. Report about two cases of unusual generalized granulomatosis in males, with multiple lymphomas and involvement of internal organs and brain, leading soon to death. The diagnosis was malignant histiocytosis and disseminated eosinophilic granulomatosis. The malignant histiocytosis is differentiated from malignant fibroxantoma (malignant histiocytoma). The unusual cerebral features in the second case - diagnosed as disseminated eosinophilic granulomatosis - seem to show some relations to histiocytosis X. The problems in diagnosis of generalized granulomatosis are pointed out."}
{"id": "PMID:1081315", "title": "[Histiocytosis X of the hypothalamus (author's transl)].", "content": "A case report on histiocytosis X in the hypothalamic region in a 47 years old woman is presented. There was a 10 years history of diabetes insipidus, but only immediately before the death vegetative and psychical disturbances occurred. Difficulties of the diagnosis and of the estimation of histological findings are discussed.", "contents": "[Histiocytosis X of the hypothalamus (author's transl)]. A case report on histiocytosis X in the hypothalamic region in a 47 years old woman is presented. There was a 10 years history of diabetes insipidus, but only immediately before the death vegetative and psychical disturbances occurred. Difficulties of the diagnosis and of the estimation of histological findings are discussed."}
{"id": "PMID:1081321", "title": "[Orgainc tricuspid pathology in rheumatic valvulopathies].", "content": "Surgical observations show that organic tricuspid lesions are not uncommon: 3.2% among cases of valvular surgery, 9.5% among patients with combined mitral and aortic replacements. Pure stenosis is relatively rare (7 cases) but easy to diagnose: this lesion plays a protective role on pulmonary parenchyma. There is no symptom due to congestive lungs and few pulmonary post-operative complications among these patients. Organic tricuspid insufficiency, isolated or associated to stenosis is more frequent (18 cases) but more difficult to diagnose before surgery. There seems to be no protection of the pulmonary circulation. Pulmonary arterial hypertension, clinical and radiological features of congestive lungs and post-operative pulmonary complications are usual.", "contents": "[Orgainc tricuspid pathology in rheumatic valvulopathies]. Surgical observations show that organic tricuspid lesions are not uncommon: 3.2% among cases of valvular surgery, 9.5% among patients with combined mitral and aortic replacements. Pure stenosis is relatively rare (7 cases) but easy to diagnose: this lesion plays a protective role on pulmonary parenchyma. There is no symptom due to congestive lungs and few pulmonary post-operative complications among these patients. Organic tricuspid insufficiency, isolated or associated to stenosis is more frequent (18 cases) but more difficult to diagnose before surgery. There seems to be no protection of the pulmonary circulation. Pulmonary arterial hypertension, clinical and radiological features of congestive lungs and post-operative pulmonary complications are usual."}
{"id": "PMID:1081322", "title": "A direct comparison between internally and externally measured left ventricular systolic time intervals.", "content": "In 38 cardiac patients a direct comparison was made of systolic time intervals derived from the external carotid pulse tracing and from the central aortic pressure curve, simultaneously recorded by means of a catheter-tip micromanometer. In addition the transmission time of the upstroke and the incisura of the central pulse wave to the carotid artery was studied. In spite of close linear correlations, significant differences in absolute values were observed between internally and externally measured systolic time intervals. The transmission times of upstroke and incisura of the pulse wave also differed significantly. Both findings were directly related and dependent upon the heart rate.", "contents": "A direct comparison between internally and externally measured left ventricular systolic time intervals. In 38 cardiac patients a direct comparison was made of systolic time intervals derived from the external carotid pulse tracing and from the central aortic pressure curve, simultaneously recorded by means of a catheter-tip micromanometer. In addition the transmission time of the upstroke and the incisura of the central pulse wave to the carotid artery was studied. In spite of close linear correlations, significant differences in absolute values were observed between internally and externally measured systolic time intervals. The transmission times of upstroke and incisura of the pulse wave also differed significantly. Both findings were directly related and dependent upon the heart rate."}
{"id": "PMID:1081323", "title": "A modified technique for transseptal angiocardiography: review of 186 cases.", "content": "Perforations of the left atrial or ventricular wall and extravasations of contrast medium during transseptal left heart catheterisation or angiocardiography can be eliminated by replacing the normally used transseptal catheters by Pigtail-catheters. With only 2.8% minor complications without sequelae in 181 successful studies, transseptal angiocardiography of the left heart through Pigtail-catheters is not only less hazardous than injections through the transseptal catheters used up to now, but possibly bears even less risk than direct retrograde injection into the left ventricle. The best method of showing the left atrial cavity and the mitral valve is transseptal left atrial injection. According to our findings in the evaluation of the ventricular function by means of quantitative angiocardiography, transseptal angiocardiography with injection into the left atrium is preferable to retrograde direct ventriculography, as ventricular ectopic beats were absent and supraventricular ectopic beats were as rare as 5% of cases. With this method, local disturbances of wall motion during injection could also be avoided and one or two more cycles could be evaluated before the depressant effect of contrast medium started.", "contents": "A modified technique for transseptal angiocardiography: review of 186 cases. Perforations of the left atrial or ventricular wall and extravasations of contrast medium during transseptal left heart catheterisation or angiocardiography can be eliminated by replacing the normally used transseptal catheters by Pigtail-catheters. With only 2.8% minor complications without sequelae in 181 successful studies, transseptal angiocardiography of the left heart through Pigtail-catheters is not only less hazardous than injections through the transseptal catheters used up to now, but possibly bears even less risk than direct retrograde injection into the left ventricle. The best method of showing the left atrial cavity and the mitral valve is transseptal left atrial injection. According to our findings in the evaluation of the ventricular function by means of quantitative angiocardiography, transseptal angiocardiography with injection into the left atrium is preferable to retrograde direct ventriculography, as ventricular ectopic beats were absent and supraventricular ectopic beats were as rare as 5% of cases. With this method, local disturbances of wall motion during injection could also be avoided and one or two more cycles could be evaluated before the depressant effect of contrast medium started."}
{"id": "PMID:1081324", "title": "Aortic septal defect and coronary-systemic micro-fistulae.", "content": "This report concerns a 32-year-old man, who at the age of 11, had an aortic septal defect with severe pulmonary hypertension. The defect was partially closed and the patient was left with a continuous murmur, an a-v shunt and marked diminution of pulmonary hypertension. Five years later he was asymptomatic, auscultation was normal and no shunt was found at cardiac catheterization. At 32 years of age, although asymptomatic he had abnormal \"T\" waves, and a selective coronary angiography demonstrated micro-fistulae involving the anterior descending coronary artery. It is suggested that these fistulae may be responsible for the abnormality of ventricular repolarization.", "contents": "Aortic septal defect and coronary-systemic micro-fistulae. This report concerns a 32-year-old man, who at the age of 11, had an aortic septal defect with severe pulmonary hypertension. The defect was partially closed and the patient was left with a continuous murmur, an a-v shunt and marked diminution of pulmonary hypertension. Five years later he was asymptomatic, auscultation was normal and no shunt was found at cardiac catheterization. At 32 years of age, although asymptomatic he had abnormal \"T\" waves, and a selective coronary angiography demonstrated micro-fistulae involving the anterior descending coronary artery. It is suggested that these fistulae may be responsible for the abnormality of ventricular repolarization."}
{"id": "PMID:1081327", "title": "Varicella followed by glomerulonephritis. Treatment with corticosteroids and azathioprine resulting in recurrence of varicella.", "content": "The present report outlines the clinical features of a 2-year-old boy who following varicella developed purpura of the lower extremities, transient gastrointestinal bleeding and glomerulonephritis. The triad of symptoms suggests Schonlein-Henoch Syndrome, but coagulation studies and renal biopsy did not confirm this, and varicella is thought to be the cause of the complications. Therapy with corticosteroids and azathioprine had only a minor effect on the nephritis but caused depression of serum IgG and specific antibody resulting in reinfection or reactivation of varicella.", "contents": "Varicella followed by glomerulonephritis. Treatment with corticosteroids and azathioprine resulting in recurrence of varicella. The present report outlines the clinical features of a 2-year-old boy who following varicella developed purpura of the lower extremities, transient gastrointestinal bleeding and glomerulonephritis. The triad of symptoms suggests Schonlein-Henoch Syndrome, but coagulation studies and renal biopsy did not confirm this, and varicella is thought to be the cause of the complications. Therapy with corticosteroids and azathioprine had only a minor effect on the nephritis but caused depression of serum IgG and specific antibody resulting in reinfection or reactivation of varicella."}
{"id": "PMID:1081328", "title": "Immunodeficiency and prognosis in Hodgkin's disease.", "content": "Blood lymphocytes from the majority of 33 unselected and untreated patients with Hodgkin's disease were deficient in T-lymphocytes and their DNA synthesis induced by concanvallin A mitogen and PPD antigen was impaired. The spontaneous DNA synthesis during the first 24 hours of culture was often raised. The prognostic role of the lymphocyte deficiency was evaluated in a follow-up 10-22 months after institution of therapy. The lymphocyte functions were more commonly abnormal in patients responding poorly to treatment (incomplete remission, relapse after treatment, or death) than in patients entering complete remission. The lymphocyte deficiency seems to give information about prognosis in Hodgkin's disease in addition to histopathology, clinical stage, B-symptoms and age.", "contents": "Immunodeficiency and prognosis in Hodgkin's disease. Blood lymphocytes from the majority of 33 unselected and untreated patients with Hodgkin's disease were deficient in T-lymphocytes and their DNA synthesis induced by concanvallin A mitogen and PPD antigen was impaired. The spontaneous DNA synthesis during the first 24 hours of culture was often raised. The prognostic role of the lymphocyte deficiency was evaluated in a follow-up 10-22 months after institution of therapy. The lymphocyte functions were more commonly abnormal in patients responding poorly to treatment (incomplete remission, relapse after treatment, or death) than in patients entering complete remission. The lymphocyte deficiency seems to give information about prognosis in Hodgkin's disease in addition to histopathology, clinical stage, B-symptoms and age."}
{"id": "PMID:1081329", "title": "Host specificity of DNA in haemophilus influenzae: The physiological and genetical bases of instability of restriction and modification of DNA in strain Rd.", "content": "Further investigations of the instability of restriction and modification properties of H. influenzae Rd strain were carried out. It has been shown that the instable properties of hsd Hind1 system are maintained even after transfer of this system to another H. influenzae strain. The expression of hsd Hind1 system is very sensitive to various physiological changes which do not influence the other hsd systems present in the same Rd strain. The instability of hsd Hind1 system is postulated to be connected with some regulator gene(s).", "contents": "Host specificity of DNA in haemophilus influenzae: The physiological and genetical bases of instability of restriction and modification of DNA in strain Rd. Further investigations of the instability of restriction and modification properties of H. influenzae Rd strain were carried out. It has been shown that the instable properties of hsd Hind1 system are maintained even after transfer of this system to another H. influenzae strain. The expression of hsd Hind1 system is very sensitive to various physiological changes which do not influence the other hsd systems present in the same Rd strain. The instability of hsd Hind1 system is postulated to be connected with some regulator gene(s)."}
{"id": "PMID:1081330", "title": "Intracranial neurinomas of the nerves of the jugular foramen. Report of 12 personal cases.", "content": "12 patients, 10 women and 2 men, were operated on for removal of a jugular foramen neurinoma. Removal was total in 11 cases and operative mortality was 16.6%. As there are no clinical or instrumental signs that permit sure distinction between these tumours and those of the cerebellopontine angle, only a thorough radiological study of the jugular foramen allows correct diagnosis. Often, however, the diagnosis is made during the operation.", "contents": "Intracranial neurinomas of the nerves of the jugular foramen. Report of 12 personal cases. 12 patients, 10 women and 2 men, were operated on for removal of a jugular foramen neurinoma. Removal was total in 11 cases and operative mortality was 16.6%. As there are no clinical or instrumental signs that permit sure distinction between these tumours and those of the cerebellopontine angle, only a thorough radiological study of the jugular foramen allows correct diagnosis. Often, however, the diagnosis is made during the operation."}
{"id": "PMID:1081331", "title": "[Diagnostic possibilities of positive contrast ventriculography (author's transl)].", "content": "A comparison has been made of the relative merits of air, Conray 60, and Dimer X ventriculography in 100 cases. The great virtue of positive contrast ventriculography is its superior ability to demonstrate abnormalities in the midline and in the posterior cranial fossa. Tomoventriculography using a multiple layer cassette permits exact localization of intraventricular lesions without causing additional discomfort to the patient. The subtraction technique confers additional benefits. Positive contrast ventriculography has its limitations when there are large extraventricular masses.", "contents": "[Diagnostic possibilities of positive contrast ventriculography (author's transl)]. A comparison has been made of the relative merits of air, Conray 60, and Dimer X ventriculography in 100 cases. The great virtue of positive contrast ventriculography is its superior ability to demonstrate abnormalities in the midline and in the posterior cranial fossa. Tomoventriculography using a multiple layer cassette permits exact localization of intraventricular lesions without causing additional discomfort to the patient. The subtraction technique confers additional benefits. Positive contrast ventriculography has its limitations when there are large extraventricular masses."}
{"id": "PMID:1081335", "title": "Coronary artery disease in an 18 year old with pseudoxanthoma elasticum: successful surgical therapy.", "content": "An 18 year old girl with pseudoxanthoma elasticum, a 7 year history of angina pectoris and evidence of an old anteroseptal myocardial infarction was found on coronary angiography to have three vessel coronary artery disease. A triple coronary artery-saphenous vein bypass graft was performed, and she has been asymptomatic for 1 year. Histologic examination of a segment of the right coronary artery revealed changes consistent with the vascular lesion of pseudoxanthoma elasticum.", "contents": "Coronary artery disease in an 18 year old with pseudoxanthoma elasticum: successful surgical therapy. An 18 year old girl with pseudoxanthoma elasticum, a 7 year history of angina pectoris and evidence of an old anteroseptal myocardial infarction was found on coronary angiography to have three vessel coronary artery disease. A triple coronary artery-saphenous vein bypass graft was performed, and she has been asymptomatic for 1 year. Histologic examination of a segment of the right coronary artery revealed changes consistent with the vascular lesion of pseudoxanthoma elasticum."}
{"id": "PMID:1081336", "title": "Zeta sedimentation ratio in rheumatic disease. Comparison of the zeta sedimentation ratio with the Wintrobe and Westergren sedimentation rates.", "content": "The zeta sedimentation ratios (ZSR) of 104 normal subjects and 106 rheumatology clinic patients were compared with their Westergren and Wintrobe erythrocyte sedimentation rates (ESR). The mean ZSR for normal subjects was 49.42 +/- 3.75%. The rheumatology clinic patients were separated into noninflammatory and inflammatory disease groups. The mean ZSR values for these groups were: noninflammatory, 51.60 +/- 5.33%; inflammatory, 59.43 +/- 7.32% (t = 5.5, p less than 0.001). The correlation between ZSR and ESR values was excellent. The ZSR determination has several advantages over the standard ESR in that it can be performed on blood anticoagulated with EDTA and need not be corrected for age, sex or packed cell volume. The ZSR is a rapid, reproducible test correlating well with rheumatic disease activity.", "contents": "Zeta sedimentation ratio in rheumatic disease. Comparison of the zeta sedimentation ratio with the Wintrobe and Westergren sedimentation rates. The zeta sedimentation ratios (ZSR) of 104 normal subjects and 106 rheumatology clinic patients were compared with their Westergren and Wintrobe erythrocyte sedimentation rates (ESR). The mean ZSR for normal subjects was 49.42 +/- 3.75%. The rheumatology clinic patients were separated into noninflammatory and inflammatory disease groups. The mean ZSR values for these groups were: noninflammatory, 51.60 +/- 5.33%; inflammatory, 59.43 +/- 7.32% (t = 5.5, p less than 0.001). The correlation between ZSR and ESR values was excellent. The ZSR determination has several advantages over the standard ESR in that it can be performed on blood anticoagulated with EDTA and need not be corrected for age, sex or packed cell volume. The ZSR is a rapid, reproducible test correlating well with rheumatic disease activity."}
{"id": "PMID:1081339", "title": "Relationships between neurological findings and classroom behavior.", "content": "Five hundred seventy-five children from low-income urban neighborhoods who were between 10 and 12 years of age were examined by pediatricians for certain neurological signs. Classroom teachers ranked each child according to types of behavior. Data on neurological signs found in more than 15 children and on types of classroom behavior clinically expected to be related to central nervous system defects were studied statistically. Significant positive associations were found between nystagmus and hyperactivity, mixed dominance and hyperactivity, and mixed dominance and variable day-to-day performance. Errors in moving parts of the body on verbal command were associated with distractibility and underachievement. Head circumference greater than the 90th percentile for age was associated with unvarying behavior and clumsiness; tactile agnosia with unvarying behavior; asymmetry of the eyes with hyperactivity; and asymmetrical position of the child's head with underachievement. A negative association was found between nystagmus and musical ability.", "contents": "Relationships between neurological findings and classroom behavior. Five hundred seventy-five children from low-income urban neighborhoods who were between 10 and 12 years of age were examined by pediatricians for certain neurological signs. Classroom teachers ranked each child according to types of behavior. Data on neurological signs found in more than 15 children and on types of classroom behavior clinically expected to be related to central nervous system defects were studied statistically. Significant positive associations were found between nystagmus and hyperactivity, mixed dominance and hyperactivity, and mixed dominance and variable day-to-day performance. Errors in moving parts of the body on verbal command were associated with distractibility and underachievement. Head circumference greater than the 90th percentile for age was associated with unvarying behavior and clumsiness; tactile agnosia with unvarying behavior; asymmetry of the eyes with hyperactivity; and asymmetrical position of the child's head with underachievement. A negative association was found between nystagmus and musical ability."}
{"id": "PMID:1081341", "title": "Crohn's disease of the colon. IV. Clinical features of Crohn's (ileo) colitis.", "content": "Using accepted diagnostic criteria we have selected, for study, 160 patients with Crohn's disease involving the colon. There is a remarkable discrepancy between the clinical diagnosis prior to or at the time of initial admission to this hospital and the diagnosis following definitive investigation and observation of the progression of disease. The peak age incidence occurred in the second decade. The colitis group showed a greater percentage of patients over 30 years of age. Although histopathology was not obtained in all patients, there appeared to be sparing of the ascending colon in a small percentage (9 percent) of patients with ileocolitis. Comparison of the clinical features of granulomatous disease limited to the colon and granulomatous ileocolitis shows a significantly greater incidence of extraintestinal symptoms and overt bleeding in the former. Nausea, vomiting, subacute obstruction, abdominal mass and internal fistulas were substantially more common in ileocolitis but the difference was not statistically significant. In this series retroperitoneal abscess did not occur in patients with disease localized to the colon. In the 10 patients with ileocolitis who developed an abscess, however, the site of fistula was the colon in four patients. In one of these, the abscess was left-sided.", "contents": "Crohn's disease of the colon. IV. Clinical features of Crohn's (ileo) colitis. Using accepted diagnostic criteria we have selected, for study, 160 patients with Crohn's disease involving the colon. There is a remarkable discrepancy between the clinical diagnosis prior to or at the time of initial admission to this hospital and the diagnosis following definitive investigation and observation of the progression of disease. The peak age incidence occurred in the second decade. The colitis group showed a greater percentage of patients over 30 years of age. Although histopathology was not obtained in all patients, there appeared to be sparing of the ascending colon in a small percentage (9 percent) of patients with ileocolitis. Comparison of the clinical features of granulomatous disease limited to the colon and granulomatous ileocolitis shows a significantly greater incidence of extraintestinal symptoms and overt bleeding in the former. Nausea, vomiting, subacute obstruction, abdominal mass and internal fistulas were substantially more common in ileocolitis but the difference was not statistically significant. In this series retroperitoneal abscess did not occur in patients with disease localized to the colon. In the 10 patients with ileocolitis who developed an abscess, however, the site of fistula was the colon in four patients. In one of these, the abscess was left-sided."}
{"id": "PMID:1081343", "title": "Endogenous Candida endophthalmitis leading to bilateral corneal perforation.", "content": "A premature neonate developed advanced bilateral endophthalmitis before the significance of underlying Candida sepsis was appreciated. Severe endophthalmitis resulted in corneal thinning, descemetocele formation, and perforation. The infection occurred in the clinical setting of broad-spectrum antibiotic therapy and indwelling intravenous catheters. Cultures of blood and catheter tips had been positive for Candida but were not considered significant until advanced ocular infection was noted. The septic process resulted in the infant's death after systemic amphotericin B therapy was discontinued because of renal toxicity.", "contents": "Endogenous Candida endophthalmitis leading to bilateral corneal perforation. A premature neonate developed advanced bilateral endophthalmitis before the significance of underlying Candida sepsis was appreciated. Severe endophthalmitis resulted in corneal thinning, descemetocele formation, and perforation. The infection occurred in the clinical setting of broad-spectrum antibiotic therapy and indwelling intravenous catheters. Cultures of blood and catheter tips had been positive for Candida but were not considered significant until advanced ocular infection was noted. The septic process resulted in the infant's death after systemic amphotericin B therapy was discontinued because of renal toxicity."}
{"id": "PMID:1081345", "title": "Localization of contractile-dependent Ca: comparison of Mn and verapamil in cardiac and skeletal muslce.", "content": "The effects of two excitation-contraction uncoupling agents, manganese (Mn) and verapamil, are compared in heart and fast-twitch skeletal muscle. Particular attention is given to the effect of the agents on the first contraction following a period of quiescence when the agents are administered during the quiescent period. Mn significantly diminishes dP/dt of the first beat in heart muscle, whereas verapamil has no significant effect. Neither Mn nor verapamil has a significant effect on the first postquiescent contraction in skeletal muscle, though verapamil produces a diminution of dP/dt in subsequent contractions in both tissues. A comparison of the effect of the agents on 45Ca exchange in heart cells in tissue culture indicates that Mn induces a rapid displacement of a rapidly exchangeable component of heart-cell calcium with subsequent inhibiton of influx. Verapamil, by contrast, produces no rapid displacement but only an inhibiton of influx. The functional and Ca-exchange effect of the two agents are compatible with a model which places most of the contractile-dependent Ca at the cellular surface in heart muscle and at deeper, intracellular sites in fast-twitch skeletal muscle.", "contents": "Localization of contractile-dependent Ca: comparison of Mn and verapamil in cardiac and skeletal muslce. The effects of two excitation-contraction uncoupling agents, manganese (Mn) and verapamil, are compared in heart and fast-twitch skeletal muscle. Particular attention is given to the effect of the agents on the first contraction following a period of quiescence when the agents are administered during the quiescent period. Mn significantly diminishes dP/dt of the first beat in heart muscle, whereas verapamil has no significant effect. Neither Mn nor verapamil has a significant effect on the first postquiescent contraction in skeletal muscle, though verapamil produces a diminution of dP/dt in subsequent contractions in both tissues. A comparison of the effect of the agents on 45Ca exchange in heart cells in tissue culture indicates that Mn induces a rapid displacement of a rapidly exchangeable component of heart-cell calcium with subsequent inhibiton of influx. Verapamil, by contrast, produces no rapid displacement but only an inhibiton of influx. The functional and Ca-exchange effect of the two agents are compatible with a model which places most of the contractile-dependent Ca at the cellular surface in heart muscle and at deeper, intracellular sites in fast-twitch skeletal muscle."}
{"id": "PMID:1081346", "title": "Hemodynamic consequences of delayed ventriculoconal conduction in the frog Rana catesbeiana.", "content": "We investigated the function of the conus arteriosus in the bullfrog Rana catesbeiana using a combination of anatomical and physiological techniques. Although there is a normal delay in ventriculoconal conduction and we could induce a spectrum of ventriculoconal conduction disturbances by manipulating the region of the ventriculoconal junction, we found no histological evidence of specialized conducting myocardial tissue in this region. The performance of the conus arteriosus was explored during various disturbances of ventriculoconal conduction and also during hemodynamic disturbances produced by hemorrhage and afterloading. The conus was found to contribute little to forward flow under ordinary circumstances, but its contribution increased greatly during bleeding or partial occlusion of the truncus. In contrast to the conclusion of others, no evidence could be adduced to support the idea that the conus serves as a depulsating chamber. Disparities in previous reports concerning the operation of the conus as a booster pump are attributed to special experimental circumstances.", "contents": "Hemodynamic consequences of delayed ventriculoconal conduction in the frog Rana catesbeiana. We investigated the function of the conus arteriosus in the bullfrog Rana catesbeiana using a combination of anatomical and physiological techniques. Although there is a normal delay in ventriculoconal conduction and we could induce a spectrum of ventriculoconal conduction disturbances by manipulating the region of the ventriculoconal junction, we found no histological evidence of specialized conducting myocardial tissue in this region. The performance of the conus arteriosus was explored during various disturbances of ventriculoconal conduction and also during hemodynamic disturbances produced by hemorrhage and afterloading. The conus was found to contribute little to forward flow under ordinary circumstances, but its contribution increased greatly during bleeding or partial occlusion of the truncus. In contrast to the conclusion of others, no evidence could be adduced to support the idea that the conus serves as a depulsating chamber. Disparities in previous reports concerning the operation of the conus as a booster pump are attributed to special experimental circumstances."}
{"id": "PMID:1081347", "title": "Determination of the ENa of from skin from studies of its current-voltage relationship.", "content": "Studies were done to test the idea that the emf of the sodium pump (ENa) of isolated frog skin could be estimated directly from studies of its current-voltage relationship. ENa was estimated in two ways: a) from the bidirectional sodium flux ratio according to Ussing and Zerahn, and b) from the values of open-circuit voltage, short-circuit current, and the shunt resistance estimated when the [Na] of the outer solution was reduced to zero. When the values of ENa were compared with the values of E1 obtained from the current-voltage relationships, they were found to be the same despite considerable variability in the values of ENa between skins (77-168 mV). In general, the values of ENa were considerably higher when the skins were bathed with sulfate-Ringer as compared with chloride-Ringer solution. These data are compatible with the idea that in nonedge-damaged frog skin, the unidirectional sodium fluxes were confined primarily to active transport pathways alone, and, accordingly, they may be excluded from parallel-shunt pathways.", "contents": "Determination of the ENa of from skin from studies of its current-voltage relationship. Studies were done to test the idea that the emf of the sodium pump (ENa) of isolated frog skin could be estimated directly from studies of its current-voltage relationship. ENa was estimated in two ways: a) from the bidirectional sodium flux ratio according to Ussing and Zerahn, and b) from the values of open-circuit voltage, short-circuit current, and the shunt resistance estimated when the [Na] of the outer solution was reduced to zero. When the values of ENa were compared with the values of E1 obtained from the current-voltage relationships, they were found to be the same despite considerable variability in the values of ENa between skins (77-168 mV). In general, the values of ENa were considerably higher when the skins were bathed with sulfate-Ringer as compared with chloride-Ringer solution. These data are compatible with the idea that in nonedge-damaged frog skin, the unidirectional sodium fluxes were confined primarily to active transport pathways alone, and, accordingly, they may be excluded from parallel-shunt pathways."}
{"id": "PMID:1081348", "title": "Trichomonas vaginalis in a perinephric abscess. A case report.", "content": "A patient with chronic vulvo-vaginitis due to Trichomonas vaginalis, and obstructive uropathy associated with renal calculi, developed a perinephric abscess following trauma incurred in a motorcycle accident. T. vaginalis was seen on smear and cultured from the purulent drainage from the perinephric abscess. Although T. vaginalis is commonly pathogenic only to the lower genito-urinary system, the upper urinary tract may very rarely be involved by ascending infection. If this protozoan spreads to extraluminal sites the inflammatory potential is marked, as has been found in animals with experimental infection. Examination of a fresh smear of pus may be critically important in the diagnosis of closed-space infections of unknown etiology.", "contents": "Trichomonas vaginalis in a perinephric abscess. A case report. A patient with chronic vulvo-vaginitis due to Trichomonas vaginalis, and obstructive uropathy associated with renal calculi, developed a perinephric abscess following trauma incurred in a motorcycle accident. T. vaginalis was seen on smear and cultured from the purulent drainage from the perinephric abscess. Although T. vaginalis is commonly pathogenic only to the lower genito-urinary system, the upper urinary tract may very rarely be involved by ascending infection. If this protozoan spreads to extraluminal sites the inflammatory potential is marked, as has been found in animals with experimental infection. Examination of a fresh smear of pus may be critically important in the diagnosis of closed-space infections of unknown etiology."}
{"id": "PMID:1081350", "title": "Gastro-intestinal haemorrhage from peptic ulcer. An evaluation of bloodless transfusion and early surgery.", "content": "Over a 3-year-period 72 patients with upper digestive tract haemorrhage due to peptic ulcer were transfused exclusively with isotonic sodium chloride and glucose solutions in equal quantities. The results are compared with 69 patients treated previously in whom conventional blood transfusion regimes had been used. Transfusion therapy was combined with emergency surgery involving vagotomy and draininage, with surgical haemostasis in situ. There were no significant differences between the two groups with regard to anaesthesia, surgery and post-operative managemement. Apart from anemia, there were less early and late complications in the group not receiving blood. The amount of crystalloid solutions administered varied between 7000 ml and 19,000 ml causing a diuresis of 1000 ml to 5300 ml within the first 24 hours. The use of this transfusion regime for haemodynamic re-equilibration in upper gastro-intestinal haemorrhage due to ulcer, in cases in which haemostasis can be obtained with certainty by emergency surgery, allows transfusion of stored blood and colloid solutions to be avoided and emergency surgery to be safely undertaken at the earliest moment. The severe acute anaemia which is caused appears to create no special problems.", "contents": "Gastro-intestinal haemorrhage from peptic ulcer. An evaluation of bloodless transfusion and early surgery. Over a 3-year-period 72 patients with upper digestive tract haemorrhage due to peptic ulcer were transfused exclusively with isotonic sodium chloride and glucose solutions in equal quantities. The results are compared with 69 patients treated previously in whom conventional blood transfusion regimes had been used. Transfusion therapy was combined with emergency surgery involving vagotomy and draininage, with surgical haemostasis in situ. There were no significant differences between the two groups with regard to anaesthesia, surgery and post-operative managemement. Apart from anemia, there were less early and late complications in the group not receiving blood. The amount of crystalloid solutions administered varied between 7000 ml and 19,000 ml causing a diuresis of 1000 ml to 5300 ml within the first 24 hours. The use of this transfusion regime for haemodynamic re-equilibration in upper gastro-intestinal haemorrhage due to ulcer, in cases in which haemostasis can be obtained with certainty by emergency surgery, allows transfusion of stored blood and colloid solutions to be avoided and emergency surgery to be safely undertaken at the earliest moment. The severe acute anaemia which is caused appears to create no special problems."}
{"id": "PMID:1081351", "title": "Electrostimulation in the management of chronic pain.", "content": "A new form of electrostimulation was investigated in a series of 768 patients with chronic pain. The method is based on the insertion of needle-electrodes in symmetrically selected points of the body and the passage of an electrical current of specified parameters. Treatment sessions last about 15-20 min and are repeated at intervals of 1 to 7 days. The procedure is simple and free from undesirable side effects. The investigation was based on 12000 sessions. The results of the treatment are discussed. No hypothesis of the mechanism of the beneficial effects obtained by electrostimulation is given, though the gate control theory of pain is cited.", "contents": "Electrostimulation in the management of chronic pain. A new form of electrostimulation was investigated in a series of 768 patients with chronic pain. The method is based on the insertion of needle-electrodes in symmetrically selected points of the body and the passage of an electrical current of specified parameters. Treatment sessions last about 15-20 min and are repeated at intervals of 1 to 7 days. The procedure is simple and free from undesirable side effects. The investigation was based on 12000 sessions. The results of the treatment are discussed. No hypothesis of the mechanism of the beneficial effects obtained by electrostimulation is given, though the gate control theory of pain is cited."}
{"id": "PMID:1081354", "title": "A micro-rosette test for newborns.", "content": "It is known that human T-cells can be identified by rosette formation with sheep red blood cells. The authors have developed a micro adaptation of the rosette-forming cell test with which they compared the lymphocyte distribution of 38 newborns to that of 42 older children. The percentage of lymphocytes spontaneously forming rosettes was similar in the two groups. Absolute lymphocyte counts were also similar. They suggest that this micro-rosette test could offer the clinician a rapid and inexpensive laboratory method of assessing cell-mediate immunity in the neonate.", "contents": "A micro-rosette test for newborns. It is known that human T-cells can be identified by rosette formation with sheep red blood cells. The authors have developed a micro adaptation of the rosette-forming cell test with which they compared the lymphocyte distribution of 38 newborns to that of 42 older children. The percentage of lymphocytes spontaneously forming rosettes was similar in the two groups. Absolute lymphocyte counts were also similar. They suggest that this micro-rosette test could offer the clinician a rapid and inexpensive laboratory method of assessing cell-mediate immunity in the neonate."}
{"id": "PMID:1081355", "title": "A preliminary report on regenerative healing in the equine tendon.", "content": "The concept of regenerative healing has been used to manipulate the healing process in experimental animals and clinically to augment bone healing in people after orthopedic operation. An implanted electrical device was used in an attempt to produce regenerative healing in experimentally created equine tendon injury. The bimetallic electrical implant did not produce regenerative healing under the conditions of this experiment. The mechanism of implantation and discussion of the results are included.", "contents": "A preliminary report on regenerative healing in the equine tendon. The concept of regenerative healing has been used to manipulate the healing process in experimental animals and clinically to augment bone healing in people after orthopedic operation. An implanted electrical device was used in an attempt to produce regenerative healing in experimentally created equine tendon injury. The bimetallic electrical implant did not produce regenerative healing under the conditions of this experiment. The mechanism of implantation and discussion of the results are included."}
{"id": "PMID:1081360", "title": "Emergency and elective surgical treatment of portal hypertension. A review of 23 years' experience.", "content": "A retrospective review of surgical treatment for portal hypertension during a 23-year period in a regional unit is reported and the immediate and subsequent management of patients with bleeding oesophageal varices is discussed. Fifty-four patients with recurrent varix haemorrhage uncontrolled by conservative methods have been treated by oesophageal transection with a mortality of 22.2% (26.6% for cirrhotic patients). Thirty-two per cent of the cirrhotics were alive after 2 years. Only a minority (12%) of the survivors were considered suitable for a subsequent shunt procedure. Therapeutic portacaval anastomosis has been performed on 65 patients with a 51.2% 5-year survival (43-5% for cirrhotic patients). Further haemorrhage due to shunt thrombosis occurred in 5-3% of cases. The frequent occurrence of portal-systemic encephalopathy, increasing with duration of time following a shunt, is emphasized. The high morbidity and mortality in the poor-risk cirrhotic indicated that this type of patient is unsuitable for a portal-systemic shunt and is better treated by medical means.", "contents": "Emergency and elective surgical treatment of portal hypertension. A review of 23 years' experience. A retrospective review of surgical treatment for portal hypertension during a 23-year period in a regional unit is reported and the immediate and subsequent management of patients with bleeding oesophageal varices is discussed. Fifty-four patients with recurrent varix haemorrhage uncontrolled by conservative methods have been treated by oesophageal transection with a mortality of 22.2% (26.6% for cirrhotic patients). Thirty-two per cent of the cirrhotics were alive after 2 years. Only a minority (12%) of the survivors were considered suitable for a subsequent shunt procedure. Therapeutic portacaval anastomosis has been performed on 65 patients with a 51.2% 5-year survival (43-5% for cirrhotic patients). Further haemorrhage due to shunt thrombosis occurred in 5-3% of cases. The frequent occurrence of portal-systemic encephalopathy, increasing with duration of time following a shunt, is emphasized. The high morbidity and mortality in the poor-risk cirrhotic indicated that this type of patient is unsuitable for a portal-systemic shunt and is better treated by medical means."}
{"id": "PMID:1081361", "title": "Different lymphocyte populations in rheumatoid arthritis and their relationship to anti-Ig activities.", "content": "The anti-immunoglobulin activities of rheumatoid arthritis appear to be related not only to plasma cells and their precursors, the B-lymphocytes. Evidence for interaction of IgG with T-lymphocytes and with Fc-bearing lymphocyte-like cells has now appeared. Both these cell types can mediate cytotoxicity which may also induce inflammation in addition to that provoked by rheumatoid factors and immune complexes. Rheumatoid factors and immune complexes may act primarily through the complement system. However, active inflammation in rheumatoid patients can occur without abundant immune complexes, e.g. in B-cell-deficient patients (31). In some cases this may be due to activation of the alternate complement pathway. However, the T-cell system with its series of lymphokines may also play an active part-in the inflammatory process. And both T-lymphocytes and Fc-bearing lymphocyte-like cells, the K-cells, can mediate cytotoxicity. Also in such patients further studies are needed to work out the complex interactions between these cell types and to discover the underlying cause or agent in the chronic destructive rheumatoid inflammation.", "contents": "Different lymphocyte populations in rheumatoid arthritis and their relationship to anti-Ig activities. The anti-immunoglobulin activities of rheumatoid arthritis appear to be related not only to plasma cells and their precursors, the B-lymphocytes. Evidence for interaction of IgG with T-lymphocytes and with Fc-bearing lymphocyte-like cells has now appeared. Both these cell types can mediate cytotoxicity which may also induce inflammation in addition to that provoked by rheumatoid factors and immune complexes. Rheumatoid factors and immune complexes may act primarily through the complement system. However, active inflammation in rheumatoid patients can occur without abundant immune complexes, e.g. in B-cell-deficient patients (31). In some cases this may be due to activation of the alternate complement pathway. However, the T-cell system with its series of lymphokines may also play an active part-in the inflammatory process. And both T-lymphocytes and Fc-bearing lymphocyte-like cells, the K-cells, can mediate cytotoxicity. Also in such patients further studies are needed to work out the complex interactions between these cell types and to discover the underlying cause or agent in the chronic destructive rheumatoid inflammation."}
{"id": "PMID:1081363", "title": "[Localization of the genes of 28S and 18S RNA in human somatic chromosomes by in situ hybridization].", "content": "In situ hybridization of human somatic cell chromosomes suggests that the genes coding for the 28S and 18S RNA's are located in the satellite of acrocentric chromosomes rather than in the secondary constriction, and that two minor sites exist in the centromeric region of chromosomes 1 and 2.", "contents": "[Localization of the genes of 28S and 18S RNA in human somatic chromosomes by in situ hybridization]. In situ hybridization of human somatic cell chromosomes suggests that the genes coding for the 28S and 18S RNA's are located in the satellite of acrocentric chromosomes rather than in the secondary constriction, and that two minor sites exist in the centromeric region of chromosomes 1 and 2."}
{"id": "PMID:1081359", "title": "New approach to caloric stimulation of the vestibular receptor.", "content": "A new caloric testing method is described. During continuous aural irrigation, fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. As a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. The magnitude of the caloric stimulus may be selected by choosing appropriate irrigation durations from a graph or table. Application of the test to clinical subjects demonstrated that the heat conduction model and analysis used in timing the sequence of thermal pulses was accurate. The new procedure causes less patient discomfort and requires less time to complete than does the conventional Fitzgerald-Hallpike test.", "contents": "New approach to caloric stimulation of the vestibular receptor. A new caloric testing method is described. During continuous aural irrigation, fluid is switched between hot and cold values at times computed according to a mathematical model of heat conduction in the labyrinth area. As a result, the induced temperature difference across the lateral semicircular canal describes an approximately sinusoidal time course, reaching peak values of equal magnitude but opposite sign. The magnitude of the caloric stimulus may be selected by choosing appropriate irrigation durations from a graph or table. Application of the test to clinical subjects demonstrated that the heat conduction model and analysis used in timing the sequence of thermal pulses was accurate. The new procedure causes less patient discomfort and requires less time to complete than does the conventional Fitzgerald-Hallpike test."}
{"id": "PMID:1081364", "title": "De novo occurrence of 46,XX,t(4;13) (q31;q14) in a mentally retarded girl.", "content": "A 12 year-old mentally retarded girl with multiple congenital malformations presented with an apparently new chromosome abnormality involving a de novo balanced reciprocal translocation between part of the long arms of chromosomes 4 and 13. This was observed in 291 metaphase spreads derived from short-term leucocyte and skin fibroblast cultures.", "contents": "De novo occurrence of 46,XX,t(4;13) (q31;q14) in a mentally retarded girl. A 12 year-old mentally retarded girl with multiple congenital malformations presented with an apparently new chromosome abnormality involving a de novo balanced reciprocal translocation between part of the long arms of chromosomes 4 and 13. This was observed in 291 metaphase spreads derived from short-term leucocyte and skin fibroblast cultures."}
{"id": "PMID:1081365", "title": "[Pure trisomy 9p 47,XX,+ del(9) (q11). Discovery of one cell 46,XX, del(9) (q11) in the father].", "content": "A case is reported of \"pure\" trisomy 9p: 47,XX,+del(9) (q11). The affected 6-year-old girl has moderate psychomotor retardation (IQ near 70), with speech retardation. She is mildly dysmorphic, with the characteristic features of trisomy 9p: a \"worried look\", a unilateral grin, slant of the palpebral fissures, a globulous nose, brachymesophalangia and the characteristic dermatoglyphic features. The parents karyotypes are normal, except for one cell from the father which had the karyotype 46,XY,del(9)(q11), the implications of which are discussed.", "contents": "[Pure trisomy 9p 47,XX,+ del(9) (q11). Discovery of one cell 46,XX, del(9) (q11) in the father]. A case is reported of \"pure\" trisomy 9p: 47,XX,+del(9) (q11). The affected 6-year-old girl has moderate psychomotor retardation (IQ near 70), with speech retardation. She is mildly dysmorphic, with the characteristic features of trisomy 9p: a \"worried look\", a unilateral grin, slant of the palpebral fissures, a globulous nose, brachymesophalangia and the characteristic dermatoglyphic features. The parents karyotypes are normal, except for one cell from the father which had the karyotype 46,XY,del(9)(q11), the implications of which are discussed."}
{"id": "PMID:1081362", "title": "[Proceedings: Measurement of the secretory activity of the interrenal glands in Rana esculenta L (author's transl)].", "content": "In the green frog, the total corticosterone concentration measured by radiocompetitive binding assay, is 4,00 +/- 0,5 mug/ml. In captivity, this level is stable; in the natural environment, it changes with the hour and the date of the sampling. The percentage of bound corticosterone is about 30%.", "contents": "[Proceedings: Measurement of the secretory activity of the interrenal glands in Rana esculenta L (author's transl)]. In the green frog, the total corticosterone concentration measured by radiocompetitive binding assay, is 4,00 +/- 0,5 mug/ml. In captivity, this level is stable; in the natural environment, it changes with the hour and the date of the sampling. The percentage of bound corticosterone is about 30%."}
{"id": "PMID:1081366", "title": "Brachydactyly type B and symphalangism in different members of a Mexican family.", "content": "A patient with typical brachydactyly type B is described. By history, 4 generations had some affected members and it was possible to examine a sister and 2 children of the proposita. These individuals in addition to the brachydactyly had symphalangism, an abnormality not previously described in association with brachydactyly type B.", "contents": "Brachydactyly type B and symphalangism in different members of a Mexican family. A patient with typical brachydactyly type B is described. By history, 4 generations had some affected members and it was possible to examine a sister and 2 children of the proposita. These individuals in addition to the brachydactyly had symphalangism, an abnormality not previously described in association with brachydactyly type B."}
{"id": "PMID:1081367", "title": "A case of ring 18 chromosome in a sibship with multiple spontaneous abortions.", "content": "We report the study of a female infant with physical stigmata suggestive of 18 chromosomes deletion, in whom cytogenetic studies revealed a 446,XX,r(18) complement. She was the last born of a sibship of seven composed otherwise by three spontaneous abortions, two perinatal deaths and one living female. The chromosome studies of the parents were normal. The cytogenetic finding and the phenotype are discussed in relation to the 18 chromosome deletion syndromes. The phenotype of the propositus would indicate that her ring 18 is significantly deficient of long arm segment. The apparent sporadic occurrence of this chromosomal anomaly in this family is discussed.", "contents": "A case of ring 18 chromosome in a sibship with multiple spontaneous abortions. We report the study of a female infant with physical stigmata suggestive of 18 chromosomes deletion, in whom cytogenetic studies revealed a 446,XX,r(18) complement. She was the last born of a sibship of seven composed otherwise by three spontaneous abortions, two perinatal deaths and one living female. The chromosome studies of the parents were normal. The cytogenetic finding and the phenotype are discussed in relation to the 18 chromosome deletion syndromes. The phenotype of the propositus would indicate that her ring 18 is significantly deficient of long arm segment. The apparent sporadic occurrence of this chromosomal anomaly in this family is discussed."}
{"id": "PMID:1081369", "title": "[Increase of the LDH-B activity in a boy with 12p trisomy by malsegregation of a maternal translocation t(12;14) (q12;p11)].", "content": "A newborn male trisomic for 12p is compared with three other 12p trisomics already reported in the literature, as well as with three patients monosomic for 12p. A \"type and countertype\" opposition is observed for five characters: in the trisomy, turricephaly, shortness of the nose, protruding anthelix, wide palms, and increased LDH-B activity; in the monosomy, protruding occiput, large nose, hypoplasia of the anthelix, narrow palms, and decreased LDH-B activity.", "contents": "[Increase of the LDH-B activity in a boy with 12p trisomy by malsegregation of a maternal translocation t(12;14) (q12;p11)]. A newborn male trisomic for 12p is compared with three other 12p trisomics already reported in the literature, as well as with three patients monosomic for 12p. A \"type and countertype\" opposition is observed for five characters: in the trisomy, turricephaly, shortness of the nose, protruding anthelix, wide palms, and increased LDH-B activity; in the monosomy, protruding occiput, large nose, hypoplasia of the anthelix, narrow palms, and decreased LDH-B activity."}
{"id": "PMID:1081370", "title": "The 12p trisomy syndrome.", "content": "Trisomy for the short arm of chromosome number 12 was diagnosed (by a G-banding method) in a girl with multiple congenital defects. Her mother and two sisters showed a balanced translocation 46,XX,rcp(12;21)(p11;p11), so, the affected girl was the result of a maternal adjacen t-1 meiotic segregation with a karyotype 46,XX,der21,rcp(12;12)(p11;q11)mat. Another sister decreased at 3 yr of age showed similar phenotypical features and was considered also affected although no karyotype studies were performed. Both affected cases were compared with a previous one and the concordant characteristics allowed the individualization of the following syndrome: severe mental retardation, peculiar flat facies with prominent checks, epicanthic folds, broad and irregular implantation of the eyebrows, broad and flat nasal bridge with short and narrow nose, anteverted nostrils and large philtrum, broad and prominent lower lip, low set ears with folded helix, prominent anthelix and deep concha, \"spade\" shape fingers (sharp-pointed distal phalanges) with shortness of the fifth, bilateral genu valgum, slightly increased space between first and second toes, secral dimple, generalized hypotonia and hyporeflexia of knees and ankles, nistagmus, retarded and dysrythmic bone age, simian creases or equivalent and distal axial triradii.", "contents": "The 12p trisomy syndrome. Trisomy for the short arm of chromosome number 12 was diagnosed (by a G-banding method) in a girl with multiple congenital defects. Her mother and two sisters showed a balanced translocation 46,XX,rcp(12;21)(p11;p11), so, the affected girl was the result of a maternal adjacen t-1 meiotic segregation with a karyotype 46,XX,der21,rcp(12;12)(p11;q11)mat. Another sister decreased at 3 yr of age showed similar phenotypical features and was considered also affected although no karyotype studies were performed. Both affected cases were compared with a previous one and the concordant characteristics allowed the individualization of the following syndrome: severe mental retardation, peculiar flat facies with prominent checks, epicanthic folds, broad and irregular implantation of the eyebrows, broad and flat nasal bridge with short and narrow nose, anteverted nostrils and large philtrum, broad and prominent lower lip, low set ears with folded helix, prominent anthelix and deep concha, \"spade\" shape fingers (sharp-pointed distal phalanges) with shortness of the fifth, bilateral genu valgum, slightly increased space between first and second toes, secral dimple, generalized hypotonia and hyporeflexia of knees and ankles, nistagmus, retarded and dysrythmic bone age, simian creases or equivalent and distal axial triradii."}
{"id": "PMID:1081371", "title": "Partial deletion of the short arm of chromosome 12(p11; p13). Report of a case.", "content": "Chromosome analysis of a 15-day old boy with multiple malformations revealed a partial deletion 12(p11;p13).", "contents": "Partial deletion of the short arm of chromosome 12(p11; p13). Report of a case. Chromosome analysis of a 15-day old boy with multiple malformations revealed a partial deletion 12(p11;p13)."}
{"id": "PMID:1081372", "title": "An inherited translocation t(4;15) (p16;q22) leading to two cases of partial trisomy 15.", "content": "A four year old girl with severe mental retardation and multiple congenital abnormalities manifested \"partial trisomy 15\". Her mother, pregnant at the time of examination, possessed a balanced translocation which, after banding techniques, was identified as t(4;15)(p16;q22). Amnio-centesis revealed the karyotype of the fetus to be identical to that of the proposita and a therapeutic abortion was performed. Prenatal investigation of a subsequent pregnancy revealed a normal male karyotype. Comparison of the proposita and aborted fetus of this family with the 5 reported other cases of \"partial trisomy 15\" does not allow for a precise recognizable clinical syndrome.", "contents": "An inherited translocation t(4;15) (p16;q22) leading to two cases of partial trisomy 15. A four year old girl with severe mental retardation and multiple congenital abnormalities manifested \"partial trisomy 15\". Her mother, pregnant at the time of examination, possessed a balanced translocation which, after banding techniques, was identified as t(4;15)(p16;q22). Amnio-centesis revealed the karyotype of the fetus to be identical to that of the proposita and a therapeutic abortion was performed. Prenatal investigation of a subsequent pregnancy revealed a normal male karyotype. Comparison of the proposita and aborted fetus of this family with the 5 reported other cases of \"partial trisomy 15\" does not allow for a precise recognizable clinical syndrome."}
{"id": "PMID:1081373", "title": "Vestibular reactions associated with minocycline.", "content": "Several recent reports have indicated that minocycline, a drug effective for meningococcal disease prophylaxis, is associated with a high incidence of vestibular side effects. In January 1975, a questionnaire on adverse reactions was completed by all 29 staff members of a North Georgia hospital who had taken minocycline at the recommended dosage after exposure to a suspected meningococcal meningitis case and by 25 age-, sex-, and occupation-matched non-treated controls. In the treated group 86% experienced moderate to severe vestibular symptoms; 84% of the symptomatic persons experienced their onset after one or two doses of 100 mg. Fifty-two percent of the treated staff members stopped taking the medication because of these effects. The symptoms resolved within 48 h after affected persons discontinued the medication. Until the safety of minocycline can be reestablished, rifampin is recommended for meningococcal disease prophylaxis, and alternative drugs are encouraged for other indications.", "contents": "Vestibular reactions associated with minocycline. Several recent reports have indicated that minocycline, a drug effective for meningococcal disease prophylaxis, is associated with a high incidence of vestibular side effects. In January 1975, a questionnaire on adverse reactions was completed by all 29 staff members of a North Georgia hospital who had taken minocycline at the recommended dosage after exposure to a suspected meningococcal meningitis case and by 25 age-, sex-, and occupation-matched non-treated controls. In the treated group 86% experienced moderate to severe vestibular symptoms; 84% of the symptomatic persons experienced their onset after one or two doses of 100 mg. Fifty-two percent of the treated staff members stopped taking the medication because of these effects. The symptoms resolved within 48 h after affected persons discontinued the medication. Until the safety of minocycline can be reestablished, rifampin is recommended for meningococcal disease prophylaxis, and alternative drugs are encouraged for other indications."}
{"id": "PMID:1081374", "title": "Double-antibody radioimmunoassay for staphylococcal enterotoxin C2.", "content": "A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxin C2 is described. The assay procedure employs anti-rabbit gamma globulin, prepared in goats, to precipitate the antigen-antibody complex of enterotoxin C2 and anti-enterotoxin C2. The test is sensitive to 100 pg of enterotoxin.", "contents": "Double-antibody radioimmunoassay for staphylococcal enterotoxin C2. A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxin C2 is described. The assay procedure employs anti-rabbit gamma globulin, prepared in goats, to precipitate the antigen-antibody complex of enterotoxin C2 and anti-enterotoxin C2. The test is sensitive to 100 pg of enterotoxin."}
{"id": "PMID:1081376", "title": "Eosinophil-specific and other granulocyte-specific antinuclear antibodies in juvenile chronic polyarthritis and adult rheumatoid arthritis.", "content": "Sera from 151 children of whom 112 had juvenile chronic polyarthritis (JCP), and from adults with rheumatoid arthritis (RA), and from healthy pregnant females were tested for the presence of granulocyte-specific antinuclear antibodies (GS-ANA). These were detected in 20% of sera from cases of JCP, in 68% of adult RA, but in none of the controls. Eosinophil-specific ANA were the only ANA present in 18% of positive children and 54% of the positive adults. GS-ANA in children were predominantly IgG and of low titre. Heat-stable GS-ANA were detected in sera from eight children but none bound complement. The presence of GS-ANA was not significantly associated with sex, age of onset, duration of disease, mean active joint count, mean ESR, nor with the presence of fever, rash, splenomegaly, amyloidosis, pericarditis, or rheumatoid factor.", "contents": "Eosinophil-specific and other granulocyte-specific antinuclear antibodies in juvenile chronic polyarthritis and adult rheumatoid arthritis. Sera from 151 children of whom 112 had juvenile chronic polyarthritis (JCP), and from adults with rheumatoid arthritis (RA), and from healthy pregnant females were tested for the presence of granulocyte-specific antinuclear antibodies (GS-ANA). These were detected in 20% of sera from cases of JCP, in 68% of adult RA, but in none of the controls. Eosinophil-specific ANA were the only ANA present in 18% of positive children and 54% of the positive adults. GS-ANA in children were predominantly IgG and of low titre. Heat-stable GS-ANA were detected in sera from eight children but none bound complement. The presence of GS-ANA was not significantly associated with sex, age of onset, duration of disease, mean active joint count, mean ESR, nor with the presence of fever, rash, splenomegaly, amyloidosis, pericarditis, or rheumatoid factor."}
{"id": "PMID:1081377", "title": "Rubella antibody levels in juvenile rheumatoid arthritis.", "content": "Increased rubella antibody titres have been reported in patients with juvenile rheumatoid arthritis (JRA) and it has been suggested that rubella virus may be of importance in the aetiology or pathogenesis of the disease. In the present study, rubella and rubeola antibody titres in 85 patients with JRA were compared to age- and sex-matched controls. 41% of the patients did not have rubella antibody, but the geometric mean titre of those with JRA who had antibody was slightly higher than that of the controls with antibody (58-9 against 42-7; P less than 0-05). The level of rubella antibody titre correlated with serum IgG levels. There was no difference in rubeola antibody titres between patients and controls, and rubeola antibody did not correlate with serum IgG. Fifteen JRA patients developed rubella antibody after rubella vaccine or natural disease. This did not result in unusually high antibody titres and was associated with a mild exacerbation of symptoms in only two patients. This study suggests that the slight increase in rubella antibody in JRA is a nonspecific manifestation of increased immunoglobulins.", "contents": "Rubella antibody levels in juvenile rheumatoid arthritis. Increased rubella antibody titres have been reported in patients with juvenile rheumatoid arthritis (JRA) and it has been suggested that rubella virus may be of importance in the aetiology or pathogenesis of the disease. In the present study, rubella and rubeola antibody titres in 85 patients with JRA were compared to age- and sex-matched controls. 41% of the patients did not have rubella antibody, but the geometric mean titre of those with JRA who had antibody was slightly higher than that of the controls with antibody (58-9 against 42-7; P less than 0-05). The level of rubella antibody titre correlated with serum IgG levels. There was no difference in rubeola antibody titres between patients and controls, and rubeola antibody did not correlate with serum IgG. Fifteen JRA patients developed rubella antibody after rubella vaccine or natural disease. This did not result in unusually high antibody titres and was associated with a mild exacerbation of symptoms in only two patients. This study suggests that the slight increase in rubella antibody in JRA is a nonspecific manifestation of increased immunoglobulins."}
{"id": "PMID:1081378", "title": "Antinative DNA antibodies as a reaction to pyrazole drugs.", "content": "A case history is presented of the occurrence of a high binding capacity for native DNA in the serum of a patient on phenylbutazone. This reverted to normal on stopping the drug. The patient also had a reversible neutropenia and leucopenia, and it is suggested that the high anti-DNA binding capacity was a feature of a drug-induced lupus-like phenomenon.", "contents": "Antinative DNA antibodies as a reaction to pyrazole drugs. A case history is presented of the occurrence of a high binding capacity for native DNA in the serum of a patient on phenylbutazone. This reverted to normal on stopping the drug. The patient also had a reversible neutropenia and leucopenia, and it is suggested that the high anti-DNA binding capacity was a feature of a drug-induced lupus-like phenomenon."}
{"id": "PMID:1081379", "title": "Erosive hemorrhagic gastroduodenitis with fibrinolysis and low factor XIII.", "content": "Four patients with erosive hemorrhagic gastroduodenitis were found to have high fibrinolytic activity of the gastric juice. No increase in the fibrinolytic activity could be demonstrated in the circulating blood, but the values found for fibrinogen, plasminogen and alpha2-macroglobulin were low. A high content of FDP was found in the serum. All patients had a markedly decreased content of factor XIII. Platelet count and other coagulation components were normal. These findings were interpreted as signs of local fibrinolysis in the diseased parts of the gastrointestinal canal. The bleeding stopped after oral and intravenous administration of a fibrinolytic inhibitor (AMCA Cyclokapron) and of factor XIII-containing concentrate. In bleeding from gastroduodenal ulcer and esophageal varices, no increase in gastric fibrinolytic activity was found. It is suggested that the high local fibrinolytic activity in the stomach in erosive gastritis together with the low content of factor XIII contributes substantially to the hemorrhage in this condition. These observations may lead to a revision of the treatment of such cases.", "contents": "Erosive hemorrhagic gastroduodenitis with fibrinolysis and low factor XIII. Four patients with erosive hemorrhagic gastroduodenitis were found to have high fibrinolytic activity of the gastric juice. No increase in the fibrinolytic activity could be demonstrated in the circulating blood, but the values found for fibrinogen, plasminogen and alpha2-macroglobulin were low. A high content of FDP was found in the serum. All patients had a markedly decreased content of factor XIII. Platelet count and other coagulation components were normal. These findings were interpreted as signs of local fibrinolysis in the diseased parts of the gastrointestinal canal. The bleeding stopped after oral and intravenous administration of a fibrinolytic inhibitor (AMCA Cyclokapron) and of factor XIII-containing concentrate. In bleeding from gastroduodenal ulcer and esophageal varices, no increase in gastric fibrinolytic activity was found. It is suggested that the high local fibrinolytic activity in the stomach in erosive gastritis together with the low content of factor XIII contributes substantially to the hemorrhage in this condition. These observations may lead to a revision of the treatment of such cases."}
{"id": "PMID:1081380", "title": "Results of direct coronary artery graft reconstruction. A five-year clinical and arteriographic appraisal.", "content": "Three hundred thirty-six patients with severe coronary artery disease were operated upon between June, 1969, and December, 1974. All were followed from 1 to 66 months (average, 37 months) with respect to survival and late myocardial infarction. The patients operated upon were compared statistically with a group of unoperated patients, and this evaluation demonstrated significantly better late survival in the surgically treated patients who had double- and triple-vessel disease. Two hundred thirty-six consecutive patients had clinical and late arteriographic reevaluation more than 6 months postoperatively; 78% were asymptomatic. Factors affecting graft patency are reviewed in detail. Late graft patency is determined by preoperative selection of vessels for grafting. Patency is not an index of success for this procedure. Surgical therapy appears to be the treatment of choice for patients with two- three-vessel coronary occlusive disease.", "contents": "Results of direct coronary artery graft reconstruction. A five-year clinical and arteriographic appraisal. Three hundred thirty-six patients with severe coronary artery disease were operated upon between June, 1969, and December, 1974. All were followed from 1 to 66 months (average, 37 months) with respect to survival and late myocardial infarction. The patients operated upon were compared statistically with a group of unoperated patients, and this evaluation demonstrated significantly better late survival in the surgically treated patients who had double- and triple-vessel disease. Two hundred thirty-six consecutive patients had clinical and late arteriographic reevaluation more than 6 months postoperatively; 78% were asymptomatic. Factors affecting graft patency are reviewed in detail. Late graft patency is determined by preoperative selection of vessels for grafting. Patency is not an index of success for this procedure. Surgical therapy appears to be the treatment of choice for patients with two- three-vessel coronary occlusive disease."}
{"id": "PMID:1081381", "title": "Cardiac vein myocardial revascularization. An experimental study and report of 3 clinical cases.", "content": "The feasibility of utilizing the coronary venous system for myocardial revascularization was explored in 18 dog experiments and 3 clinical patients at St. Mary Medical Center. Experimental models were developed using mammary artery-to-coronary vein anastomoses, free vein grafts from the aorta to the coronary veins, and saphenous vein bypass grafts from the aorta to the cardiac veins in the patients. Evaluation of myocardial revascularization was done by one or more of the following methods: (1) electromagnetic flowmeter measurements of graft blood flow; (2) myocardial scanning after injection of radioactive materials; (3) hydrogen electrode evaluation of arteriovenous shunting; (4) coronary cineangiograms; (5) methylene blue injections with visual observation of myocardial staining and collateral venous pathways; (6) pulse-flow tracings; (7) electrocardiographic changes; and (8) myocardial venous capillary response to papaverine and isoproterenol. The experimental studies consistently demonstrated evidence of myocardial revascularization through the coronary venous system. Three patients with intractable angina pectoris and previous unsuccessful revascularization procedures underwent saphenous vein bypass grafting from the aorta to the coronary vein. Postoperative coronary cineangiograms showed patency in 2 of 4 grafts. Myocardial scanning demonstrated radioactivity in the regions served by the patent grafts. All patients survived and were partially or completely relieved of their symptoms.", "contents": "Cardiac vein myocardial revascularization. An experimental study and report of 3 clinical cases. The feasibility of utilizing the coronary venous system for myocardial revascularization was explored in 18 dog experiments and 3 clinical patients at St. Mary Medical Center. Experimental models were developed using mammary artery-to-coronary vein anastomoses, free vein grafts from the aorta to the coronary veins, and saphenous vein bypass grafts from the aorta to the cardiac veins in the patients. Evaluation of myocardial revascularization was done by one or more of the following methods: (1) electromagnetic flowmeter measurements of graft blood flow; (2) myocardial scanning after injection of radioactive materials; (3) hydrogen electrode evaluation of arteriovenous shunting; (4) coronary cineangiograms; (5) methylene blue injections with visual observation of myocardial staining and collateral venous pathways; (6) pulse-flow tracings; (7) electrocardiographic changes; and (8) myocardial venous capillary response to papaverine and isoproterenol. The experimental studies consistently demonstrated evidence of myocardial revascularization through the coronary venous system. Three patients with intractable angina pectoris and previous unsuccessful revascularization procedures underwent saphenous vein bypass grafting from the aorta to the coronary vein. Postoperative coronary cineangiograms showed patency in 2 of 4 grafts. Myocardial scanning demonstrated radioactivity in the regions served by the patent grafts. All patients survived and were partially or completely relieved of their symptoms."}
{"id": "PMID:1081382", "title": "Opiate drug receptors on excitable cell membranes.", "content": "In drug interaction studies on intact mice it was found that naloxone (3mumoles/kg) antagonized the general central nervous system depressant effects of morphine or meperidine. Higher naloxone doses (0.3 and 0.4 mmoles/kg), like the fullagonists, potentiated the loss of the righting reflex produced by phenobarbital. Naloxone (0.3 to 14 muM) antagonized the depressant effects of morphine or meperidine on the compound action potential of frog's sartorius muscles in vitro. Higher naloxone concentration (ED20=0.3 mM, ED100=3.0mM), like the full agonists, depressed action potential production. These results show that high doses of naloxone can duplicate some effects of morphine or meperidine but at low doses it antagonizes the effects of the full agonists. This would suggest that naloxone is a partial agonist with a very low intrinsic activity. These results also indicate that there are opiate drug receptors on excitable cell membranes and that drug activation of these receptors inhibits action potential production.", "contents": "Opiate drug receptors on excitable cell membranes. In drug interaction studies on intact mice it was found that naloxone (3mumoles/kg) antagonized the general central nervous system depressant effects of morphine or meperidine. Higher naloxone doses (0.3 and 0.4 mmoles/kg), like the fullagonists, potentiated the loss of the righting reflex produced by phenobarbital. Naloxone (0.3 to 14 muM) antagonized the depressant effects of morphine or meperidine on the compound action potential of frog's sartorius muscles in vitro. Higher naloxone concentration (ED20=0.3 mM, ED100=3.0mM), like the full agonists, depressed action potential production. These results show that high doses of naloxone can duplicate some effects of morphine or meperidine but at low doses it antagonizes the effects of the full agonists. This would suggest that naloxone is a partial agonist with a very low intrinsic activity. These results also indicate that there are opiate drug receptors on excitable cell membranes and that drug activation of these receptors inhibits action potential production."}
{"id": "PMID:1081383", "title": "Effect of helium on membrane resistance.", "content": "Resistance measurements were made of bimolecular lipid membranes (BLM) according to Mueller and Rudin (10). With the addition of excitability inducing material (EIM), a bacterial protein, the electrical resistance of the BLM was reduced to within biological limits. Frog pericardium was used in the same system as a natural membrane comparison. Exposure of these membranes to air, oxygen and nitrogen resulted in no significant change in the resistance of the bilayers or the pericardium. Exposure to helium and helium 79%-oxygen 21% mixutre resulted in a significant increase in the membrane resistance.", "contents": "Effect of helium on membrane resistance. Resistance measurements were made of bimolecular lipid membranes (BLM) according to Mueller and Rudin (10). With the addition of excitability inducing material (EIM), a bacterial protein, the electrical resistance of the BLM was reduced to within biological limits. Frog pericardium was used in the same system as a natural membrane comparison. Exposure of these membranes to air, oxygen and nitrogen resulted in no significant change in the resistance of the bilayers or the pericardium. Exposure to helium and helium 79%-oxygen 21% mixutre resulted in a significant increase in the membrane resistance."}
{"id": "PMID:1081384", "title": "Cytoplasmic and outer membranes separation in Rhodopseudomonas sphaeroides.", "content": "A cell envelope fraction had been prepared after mechanical disruption of lysozyme-EDTA spheroplasts from depigmented Rhodopseudomonas sphaeroides (aerobically grown in the light). On linear sucrose gradients this fraction can be separated in a cytoplasmic membrane fraction and an outer membrane fraction. The cytoplasmic fraction (buoyant density: 1.18 g/cm3) has been characterized by its succinic dehydrogenase activity and by its composition. The outer membrane fraction (buoyant density: 1.21 g/cm3) does not contain any respiratory activity nor hemoproteins. The same fractionation has been done on cells repigmented in the dark by lowering the O2 pressure. In that case the same two fractions have been detected in addition to the chromatophore fraction (buoyant density: 1.14 g/cm3). However both, and specially the outer membrane fraction, were contaminated by chromatophore material.", "contents": "Cytoplasmic and outer membranes separation in Rhodopseudomonas sphaeroides. A cell envelope fraction had been prepared after mechanical disruption of lysozyme-EDTA spheroplasts from depigmented Rhodopseudomonas sphaeroides (aerobically grown in the light). On linear sucrose gradients this fraction can be separated in a cytoplasmic membrane fraction and an outer membrane fraction. The cytoplasmic fraction (buoyant density: 1.18 g/cm3) has been characterized by its succinic dehydrogenase activity and by its composition. The outer membrane fraction (buoyant density: 1.21 g/cm3) does not contain any respiratory activity nor hemoproteins. The same fractionation has been done on cells repigmented in the dark by lowering the O2 pressure. In that case the same two fractions have been detected in addition to the chromatophore fraction (buoyant density: 1.14 g/cm3). However both, and specially the outer membrane fraction, were contaminated by chromatophore material."}
{"id": "PMID:1081386", "title": "Postinfarction ventricular aneurysm. Successful surgical treatment.", "content": "Over a two-year period, 18 patients with postinfarction ventricular aneurysms were treated surgically. There were no operative or early deaths, and only one (5.5%) late (20 months) death. The indications for surgery included angina, congestive heart failure, and serious arrhythmias. Electrocardiograms and plain chest roentgenograms were unreliable in making the diagnosis. All patients had cardiac catheterization and, at operation, a definite localized aneurysm was demonstrable. In 15 patients (83%), coronary artery bypass grafts were performed at the time the aneurysm was excised. Good results should be expected from surgical treatment of symptomatic ventricular aneurysms. Simultaneous bypass grafting should be performed when indicated.", "contents": "Postinfarction ventricular aneurysm. Successful surgical treatment. Over a two-year period, 18 patients with postinfarction ventricular aneurysms were treated surgically. There were no operative or early deaths, and only one (5.5%) late (20 months) death. The indications for surgery included angina, congestive heart failure, and serious arrhythmias. Electrocardiograms and plain chest roentgenograms were unreliable in making the diagnosis. All patients had cardiac catheterization and, at operation, a definite localized aneurysm was demonstrable. In 15 patients (83%), coronary artery bypass grafts were performed at the time the aneurysm was excised. Good results should be expected from surgical treatment of symptomatic ventricular aneurysms. Simultaneous bypass grafting should be performed when indicated."}
{"id": "PMID:1081387", "title": "Retroperitoneal approach for portasystemic decompression.", "content": "A left retroperitoneal approach has been developed and used to perform a renal-splenic shunt for portal decompression in seven patients with massive variceal hemorrhage. In each patient, at least one intra-abdominal finding contraindicated a conventional transperitoneal portasystemic shunt. Retroperitoneal approach avoided possible complications and permitted successful portal decompression with cessation of bleeding in each instance. These results support the use of this technique when it is necessary to avoid the peritoneal cavity in a patient requiring portasystemic decompression.", "contents": "Retroperitoneal approach for portasystemic decompression. A left retroperitoneal approach has been developed and used to perform a renal-splenic shunt for portal decompression in seven patients with massive variceal hemorrhage. In each patient, at least one intra-abdominal finding contraindicated a conventional transperitoneal portasystemic shunt. Retroperitoneal approach avoided possible complications and permitted successful portal decompression with cessation of bleeding in each instance. These results support the use of this technique when it is necessary to avoid the peritoneal cavity in a patient requiring portasystemic decompression."}
{"id": "PMID:1081388", "title": "Long-term survival following coronary artery bypass. Analysis of 4,522 consecutive patients.", "content": "A consecutive series of 4,522 patients who received aortocoronary bypass (ACB) from October 1969 through December 1974 has been analyzed with respect to cumulative (actuarial) survival, cause of late death, and late postoperative complications. Through December 1973, 2,676 patients received ACB alone. Cumulative survival was 85.9% for five years. Late cumulative mortality averaged 2.7% per year. Causes of death were myocardial in origin in only 60.4% of the patients. Late complications of nonfatal myocardial infarction occurred in 4.1% of the patients, and congestive heart failure occurred in 10.2%. However, preoperative congestive heart failure was present in 25% of these subjects. Mortality in 1974 was 3.4% in patients who underwent ACB only, and 4.2% for all patients who received ACB procedures (1,478 patients). Comparison to previous medical data makes it appear that this surgery increased the actuarial survival over long-term follow-up.", "contents": "Long-term survival following coronary artery bypass. Analysis of 4,522 consecutive patients. A consecutive series of 4,522 patients who received aortocoronary bypass (ACB) from October 1969 through December 1974 has been analyzed with respect to cumulative (actuarial) survival, cause of late death, and late postoperative complications. Through December 1973, 2,676 patients received ACB alone. Cumulative survival was 85.9% for five years. Late cumulative mortality averaged 2.7% per year. Causes of death were myocardial in origin in only 60.4% of the patients. Late complications of nonfatal myocardial infarction occurred in 4.1% of the patients, and congestive heart failure occurred in 10.2%. However, preoperative congestive heart failure was present in 25% of these subjects. Mortality in 1974 was 3.4% in patients who underwent ACB only, and 4.2% for all patients who received ACB procedures (1,478 patients). Comparison to previous medical data makes it appear that this surgery increased the actuarial survival over long-term follow-up."}
{"id": "PMID:1081389", "title": "[The acute vestibular paralysis (author's transl)].", "content": "Acute vestibular paralysis may not be considered as a nosologic entity but as a syndrome. Symptomatology (vertigo, spontaneous and provoked vestibular nystagmus, absence of cochlear signs) shows an uniform picture. The results of the caloric test as well as the nystagmic responses induced by galvanic stimulation and the development of central vestibular compensation however indicate that the site of the lesion is not only confined to the labyrinth but may also occur at the level of the peripheral neuron or even the vestibular nuclei. Etiology and pathology are still vague. Our own clinical observations as well as the scarce data in literature about morphological and experimental studies suggest in a way that vascular and infectious disorders are of importance as primary releasing factors. Hypothetically, vestibular loss of function may either be caused by a disturbance of labyrinthine microcirculation, initiated in a great majority of cases by infection, or by a direct lesion of the peripheral neuron as well as the vestibular nuclei. Retrolabyrinthine lesions may be due to menigoencephalitis, caused by a neurotropic virus or other infectious agents such as Toxoplasma gondii. Acute vestibular paralysis should be strictly distinguished from vestibular neuronitis. While vestibular paralysis is a syndrome, vestibular neuronitis must be considered as a nosologic entity, including a lesion of the peripheral neuron as well as evidence of an infectious event.", "contents": "[The acute vestibular paralysis (author's transl)]. Acute vestibular paralysis may not be considered as a nosologic entity but as a syndrome. Symptomatology (vertigo, spontaneous and provoked vestibular nystagmus, absence of cochlear signs) shows an uniform picture. The results of the caloric test as well as the nystagmic responses induced by galvanic stimulation and the development of central vestibular compensation however indicate that the site of the lesion is not only confined to the labyrinth but may also occur at the level of the peripheral neuron or even the vestibular nuclei. Etiology and pathology are still vague. Our own clinical observations as well as the scarce data in literature about morphological and experimental studies suggest in a way that vascular and infectious disorders are of importance as primary releasing factors. Hypothetically, vestibular loss of function may either be caused by a disturbance of labyrinthine microcirculation, initiated in a great majority of cases by infection, or by a direct lesion of the peripheral neuron as well as the vestibular nuclei. Retrolabyrinthine lesions may be due to menigoencephalitis, caused by a neurotropic virus or other infectious agents such as Toxoplasma gondii. Acute vestibular paralysis should be strictly distinguished from vestibular neuronitis. While vestibular paralysis is a syndrome, vestibular neuronitis must be considered as a nosologic entity, including a lesion of the peripheral neuron as well as evidence of an infectious event."}
{"id": "PMID:1081390", "title": "The management of septic arthritis in childhood.", "content": "A prospective study of one hundred children with septic arthritis showed that the knee and hip were the joints most affected and that Staphylococcus aureus and Haemophilus influenzae Type B were the commonest bacteria grown. Our treatment, consisting of early arthrotomy and intravenous methicillin and ampicillin, followed by six weeks of joint immobilization and oral administration of cloxacillin and ampicillin, effectively reversed the inflammatory process and compared very favourably with other methods of treatment. Significant residual joint abnormalities, present in 7% of the children, resulted from severe joint damage occurring before treatment. To achieve the best results, this regime must be instituted before permanent joint damage has occurred. This will only be achieved if an early clinical diagnosis of septic arthritis is made.", "contents": "The management of septic arthritis in childhood. A prospective study of one hundred children with septic arthritis showed that the knee and hip were the joints most affected and that Staphylococcus aureus and Haemophilus influenzae Type B were the commonest bacteria grown. Our treatment, consisting of early arthrotomy and intravenous methicillin and ampicillin, followed by six weeks of joint immobilization and oral administration of cloxacillin and ampicillin, effectively reversed the inflammatory process and compared very favourably with other methods of treatment. Significant residual joint abnormalities, present in 7% of the children, resulted from severe joint damage occurring before treatment. To achieve the best results, this regime must be instituted before permanent joint damage has occurred. This will only be achieved if an early clinical diagnosis of septic arthritis is made."}
{"id": "PMID:1081391", "title": "Percutaneous radiofrequency gangliotomy in the treatment of trigeminal neuralgia and other facial pain.", "content": "Thirty-eight patients with facial pain in the trigeminal distribution have been treated by percutaneous coagulation of the trigeminal ganglion. Although use has been made of radiological criteria, it has been found from the physiological data obtained from stimulation that motor and sensory phenomena provide more information of electrode placement and that good pain relief results when these criteria are met. The advantages of the method are: (i) minimal operative risk; (ii) control of the extent of the lesion created; (iii) small sensibility deficit; (iv) minimal complication rate; (v) short hospitalization; (vi) satisfactory result in more than 90% of patients; (vii) no recurrence of pain to date.", "contents": "Percutaneous radiofrequency gangliotomy in the treatment of trigeminal neuralgia and other facial pain. Thirty-eight patients with facial pain in the trigeminal distribution have been treated by percutaneous coagulation of the trigeminal ganglion. Although use has been made of radiological criteria, it has been found from the physiological data obtained from stimulation that motor and sensory phenomena provide more information of electrode placement and that good pain relief results when these criteria are met. The advantages of the method are: (i) minimal operative risk; (ii) control of the extent of the lesion created; (iii) small sensibility deficit; (iv) minimal complication rate; (v) short hospitalization; (vi) satisfactory result in more than 90% of patients; (vii) no recurrence of pain to date."}
{"id": "PMID:1081399", "title": "Acute coronary insufficiency. An urgent surgical condition.", "content": "In 41 of 220 consecutive patients who had a coronary artery bypass operation between July 1973 and March 1974 the operation was for acute coronary insufficiency (recurrent chest pain with transient electrocardiographic changes persisting after admission to hospital). Their mean age was 54 (range 33-70 years). Eleven patients had had angina before, 14 had had at least one myocardial infarction, and 16 presented de novo. Eight of the latter 16 patients required only a single graft, usually to the left anterior descending artery, a significantly greater number than the two of the other 25 patients (P less than 0.01). Fourteen of these 16 patients had normal ventricular contraction, a significantly higher proportion than the 13 of the remaining 25 (p less than 0.05). No collaterals were seen in any of the 10 with single-vessel disease, which was significantly fewer than five out of 18 with double- and nine out of 13 with triple-vessel disease (P less than 0.005). Patients with rapidly developing obstruction, especially in the proximal left anterior descending artery, may not have time to develop collaterals,  present acutely with good ventricular function, and may be particularly at risk. There was no operative mortality. The patients had a perioperative myocardial infarction, and there was one late death. At follow-up averaging 9-7 months (range 5-14 months) 32 (80%) patients were angina-free, no myocardial infarctions had occurred, and 85% were fully employed. Urgent coronary artery bypass grafting is a safe and effective treatment for acute coronary insufficiency.", "contents": "Acute coronary insufficiency. An urgent surgical condition. In 41 of 220 consecutive patients who had a coronary artery bypass operation between July 1973 and March 1974 the operation was for acute coronary insufficiency (recurrent chest pain with transient electrocardiographic changes persisting after admission to hospital). Their mean age was 54 (range 33-70 years). Eleven patients had had angina before, 14 had had at least one myocardial infarction, and 16 presented de novo. Eight of the latter 16 patients required only a single graft, usually to the left anterior descending artery, a significantly greater number than the two of the other 25 patients (P less than 0.01). Fourteen of these 16 patients had normal ventricular contraction, a significantly higher proportion than the 13 of the remaining 25 (p less than 0.05). No collaterals were seen in any of the 10 with single-vessel disease, which was significantly fewer than five out of 18 with double- and nine out of 13 with triple-vessel disease (P less than 0.005). Patients with rapidly developing obstruction, especially in the proximal left anterior descending artery, may not have time to develop collaterals,  present acutely with good ventricular function, and may be particularly at risk. There was no operative mortality. The patients had a perioperative myocardial infarction, and there was one late death. At follow-up averaging 9-7 months (range 5-14 months) 32 (80%) patients were angina-free, no myocardial infarctions had occurred, and 85% were fully employed. Urgent coronary artery bypass grafting is a safe and effective treatment for acute coronary insufficiency."}
{"id": "PMID:1081401", "title": "Pseudoxanthomatous mastocytosis.", "content": "A case of xanthelasmoidea (pseudoxanthomatous mastocytosis) occurring in a 50-year-old Iranian man is described. The patient had a large upper gastrointestinal haemorrhage.", "contents": "Pseudoxanthomatous mastocytosis. A case of xanthelasmoidea (pseudoxanthomatous mastocytosis) occurring in a 50-year-old Iranian man is described. The patient had a large upper gastrointestinal haemorrhage."}
{"id": "PMID:1081402", "title": "Immunohistological studies, with anti-connective tissue and anti-immunoglobulin antisera, of the skin in lupus erythematosus and scleroderma.", "content": "Skin lesions from six patients with systemic lupus erythematosus, five patients with discoid lupus erythematosus, twelve patients with systemic sclerosis, five patients with localized morphoea and twenty controls were examined by immunohistological techniques using fluorescein-labelled anti-human IgG, anti-human C3 and anti-human renal glomerulus antisera. The major immunohistological changes in systemic sclerosis and in localized morphoea consisted of foci of intercollagenous staining for connective tissue antigens in the reticular layer of the dermis. It is suggested that these findings indicate collagen neogenesis. In lupus erythematosus the major changes occur in the dermo-epidermal junction and consist of deposits of IgG and C3 and thickening and disruption of the membrane as demonstrated by the use of heterologous sera containing antibasement membrane antibodies. Immunohistological techniques are useful in the diagnostic differentiation between scleroderma and lupus erythematosus.", "contents": "Immunohistological studies, with anti-connective tissue and anti-immunoglobulin antisera, of the skin in lupus erythematosus and scleroderma. Skin lesions from six patients with systemic lupus erythematosus, five patients with discoid lupus erythematosus, twelve patients with systemic sclerosis, five patients with localized morphoea and twenty controls were examined by immunohistological techniques using fluorescein-labelled anti-human IgG, anti-human C3 and anti-human renal glomerulus antisera. The major immunohistological changes in systemic sclerosis and in localized morphoea consisted of foci of intercollagenous staining for connective tissue antigens in the reticular layer of the dermis. It is suggested that these findings indicate collagen neogenesis. In lupus erythematosus the major changes occur in the dermo-epidermal junction and consist of deposits of IgG and C3 and thickening and disruption of the membrane as demonstrated by the use of heterologous sera containing antibasement membrane antibodies. Immunohistological techniques are useful in the diagnostic differentiation between scleroderma and lupus erythematosus."}
{"id": "PMID:1081403", "title": "Heterogeneity in biological activity of human factor VIII antibodies.", "content": "Antibodies against factor VIII collected from six patients were studied for their effect on factor-VIII coagulant activity, Willebrand factor activity (WF) and factor-VIII-related antigen. The results demonstrate differences between individual antibodies; some acting primarily as anti factor VIII, others inhibiting both after VIII and WF. Differences in effect between plasma and serum indicate that the process of coagulation alters the interaction of these antibodies.", "contents": "Heterogeneity in biological activity of human factor VIII antibodies. Antibodies against factor VIII collected from six patients were studied for their effect on factor-VIII coagulant activity, Willebrand factor activity (WF) and factor-VIII-related antigen. The results demonstrate differences between individual antibodies; some acting primarily as anti factor VIII, others inhibiting both after VIII and WF. Differences in effect between plasma and serum indicate that the process of coagulation alters the interaction of these antibodies."}
{"id": "PMID:1081404", "title": "The von Willebrand syndrome.", "content": "Five patients with an original diagnosis of von Willebrand's disease are described because of their levels of factor VIII related protein, Ristocetin-induced platelet aggregation and/or family studies differed from the main group of patients with classical von Willebrand's disease. Two had normal levels of factor VIII related protein with reduced Ristocetin aggregation when this was tested in platelet rich plasma. In one, however, this was due to a plasma defect and in the other to a platelet abnormality. After cryoprecipitate infusion all abnormal tests were corrected in both these patients. The first patient, however, failed to show a secondary rise of factor VIII whereas the second showed a secondary rise of both factor VIII and of factor VIII related protein. The other three cases, who were all very severely affected, have been separated from the main group as none of their families was segregating for classical von Willebrand's disease. It is suggested that the term von Willebrand's disease should be confined to those patients who have reduced factor VIII related protein and Ristocetin aggregation, and that von Willebrand's syndrome should be used for the various sub-groups that are emerging.", "contents": "The von Willebrand syndrome. Five patients with an original diagnosis of von Willebrand's disease are described because of their levels of factor VIII related protein, Ristocetin-induced platelet aggregation and/or family studies differed from the main group of patients with classical von Willebrand's disease. Two had normal levels of factor VIII related protein with reduced Ristocetin aggregation when this was tested in platelet rich plasma. In one, however, this was due to a plasma defect and in the other to a platelet abnormality. After cryoprecipitate infusion all abnormal tests were corrected in both these patients. The first patient, however, failed to show a secondary rise of factor VIII whereas the second showed a secondary rise of both factor VIII and of factor VIII related protein. The other three cases, who were all very severely affected, have been separated from the main group as none of their families was segregating for classical von Willebrand's disease. It is suggested that the term von Willebrand's disease should be confined to those patients who have reduced factor VIII related protein and Ristocetin aggregation, and that von Willebrand's syndrome should be used for the various sub-groups that are emerging."}
{"id": "PMID:1081405", "title": "Senile pseudoexfoliation in aphakic eyes.", "content": "In three eyes which underwent cataract extraction no evidence of senile pseudoexfoliation was found either before, or immediately after, surgery in spite of repeated biomicroscopical examination under full mydriasis. Years later pseudoexfoliative material was found on the anterior hyaloid and on the pupillary border. In another case, wherein the lens was traumatically dislocated and had lain in the inferior vitreous chamber for 53 years, pseudoexfoliative material was present on the anterior hyaloid, but not in the vitreous. Collating these clinical observations with data from electron and light microscopy studies, the authors refute the assumption that lens epithelium is the source of pseudoexfoliative material.", "contents": "Senile pseudoexfoliation in aphakic eyes. In three eyes which underwent cataract extraction no evidence of senile pseudoexfoliation was found either before, or immediately after, surgery in spite of repeated biomicroscopical examination under full mydriasis. Years later pseudoexfoliative material was found on the anterior hyaloid and on the pupillary border. In another case, wherein the lens was traumatically dislocated and had lain in the inferior vitreous chamber for 53 years, pseudoexfoliative material was present on the anterior hyaloid, but not in the vitreous. Collating these clinical observations with data from electron and light microscopy studies, the authors refute the assumption that lens epithelium is the source of pseudoexfoliative material."}
{"id": "PMID:1081406", "title": "Dematiaceous fungal keratitis. Clinical isolates and management.", "content": "Clinical and laboratory features of 16 cases of keratitis that were caused by dematiaceous pigmented fungi are reported. Management, including the treatment of nine cases with Natamycin (Pimaricin), resulted in corneal healing in 14 cases, and therapeutic surgery in two cases.", "contents": "Dematiaceous fungal keratitis. Clinical isolates and management. Clinical and laboratory features of 16 cases of keratitis that were caused by dematiaceous pigmented fungi are reported. Management, including the treatment of nine cases with Natamycin (Pimaricin), resulted in corneal healing in 14 cases, and therapeutic surgery in two cases."}
{"id": "PMID:1081407", "title": "The management of patients with the Kasabach-Merritt syndrome.", "content": "The Kasabach-Merritt syndrome comprises consumption coagulation associated with giant haemangioma. A case is reported of disseminated intravascular coagulation complicating dental extraction in a 36-year-old male with this syndrome. The pre-operative management with anticoagulant therapy is discussed and the relevant literature reviewed.", "contents": "The management of patients with the Kasabach-Merritt syndrome. The Kasabach-Merritt syndrome comprises consumption coagulation associated with giant haemangioma. A case is reported of disseminated intravascular coagulation complicating dental extraction in a 36-year-old male with this syndrome. The pre-operative management with anticoagulant therapy is discussed and the relevant literature reviewed."}
{"id": "PMID:1081410", "title": "Classification of lymphocytes in nasopharyngeal carcinoma (NPC) biopsies.", "content": "Seven biopsies taken from nasopharyngeal carcinoma tissues were analyzed with regard to infiltrating B or T lymphocytes. The vast majority of these cells had the capacity to form SRBC rosettes (mean 87%) and were thus classified as T cells. Varying proportions of these were blast-transformed suggesting that they represented a host response against the tumor.", "contents": "Classification of lymphocytes in nasopharyngeal carcinoma (NPC) biopsies. Seven biopsies taken from nasopharyngeal carcinoma tissues were analyzed with regard to infiltrating B or T lymphocytes. The vast majority of these cells had the capacity to form SRBC rosettes (mean 87%) and were thus classified as T cells. Varying proportions of these were blast-transformed suggesting that they represented a host response against the tumor."}
{"id": "PMID:1081408", "title": "[Study of nucleoside phosphorescence with a phosphorescent microscope].", "content": "Phosphorescence of ribonucleosides and desoxyribonucleosides is studied. The phosphorescence spectra of polycrystalline powders of adenosine, guanosine, thymidine, uridine, cytidine, desoxyadenosine, desoxyguanosine and desoxycytidine are recorded at -175 degrees C as well as the decay curves at -175 degrees C. The phosphorescence spectrum of guanosine is also recorded at room temperature. The decay times of phosphorescence of adenosine and cytidine are determined at room temperature too. Obtained values of pi for nucleosides are shorter than those for nucleic acid bases.", "contents": "[Study of nucleoside phosphorescence with a phosphorescent microscope]. Phosphorescence of ribonucleosides and desoxyribonucleosides is studied. The phosphorescence spectra of polycrystalline powders of adenosine, guanosine, thymidine, uridine, cytidine, desoxyadenosine, desoxyguanosine and desoxycytidine are recorded at -175 degrees C as well as the decay curves at -175 degrees C. The phosphorescence spectrum of guanosine is also recorded at room temperature. The decay times of phosphorescence of adenosine and cytidine are determined at room temperature too. Obtained values of pi for nucleosides are shorter than those for nucleic acid bases."}
{"id": "PMID:1081411", "title": "Measurement of the number of bone marrow multipotential stem cells in AKR leukaemic mice.", "content": "Using the anti-theta serum to eliminate leukaemic cells, it was possible to assess the number of colony-forming units in the bone marrow of AKR leukaemic mice. It was found that the number of CFUs decreased in leukaemic mice.", "contents": "Measurement of the number of bone marrow multipotential stem cells in AKR leukaemic mice. Using the anti-theta serum to eliminate leukaemic cells, it was possible to assess the number of colony-forming units in the bone marrow of AKR leukaemic mice. It was found that the number of CFUs decreased in leukaemic mice."}
{"id": "PMID:1081409", "title": "[Absorption spectra of nucleic acids and related compounds in the spectral region 120--280 nm].", "content": "The absorption spectra of thin films of nucleic acids, nucleosides, nucleotides, D-ribose, Na3PO4 in vacuum ultraviolet region are measured. In the spectral region 280--160 nm the absorption spectra consist of the bands of nucleic acid bases. In the range shorter than 160 nm the absorption is determined by phosphate and D-ribose groups. The methods of thin films preparation are discussed.", "contents": "[Absorption spectra of nucleic acids and related compounds in the spectral region 120--280 nm]. The absorption spectra of thin films of nucleic acids, nucleosides, nucleotides, D-ribose, Na3PO4 in vacuum ultraviolet region are measured. In the spectral region 280--160 nm the absorption spectra consist of the bands of nucleic acid bases. In the range shorter than 160 nm the absorption is determined by phosphate and D-ribose groups. The methods of thin films preparation are discussed."}
{"id": "PMID:1081412", "title": "[Separation of antirubella IGM antibodies by affinity chromatography].", "content": "The separation of IgM globulins by affinity chromatography has been used for serodiagnosis of primary infections with rubella or measles virus. IgM antibody activity levels are similar to those obtained when the separation has been performed by ultracentrifugation. The results of 50 cases examined by the two separation methods are in agreement. However, affinity chromatography is more specific than gel filtration methods and requires a smaller amount of serum. The anti-human IgM globulins, covalently bound to activated Sepharose, maintain their binding capacity to IgM for a long period; and the same gel could be used more than 40 times. Due to its specificity, sensitivity and reliability, affinity chromatography is an efficient tool for routine laboratory diagnosis of a recent primary infection.", "contents": "[Separation of antirubella IGM antibodies by affinity chromatography]. The separation of IgM globulins by affinity chromatography has been used for serodiagnosis of primary infections with rubella or measles virus. IgM antibody activity levels are similar to those obtained when the separation has been performed by ultracentrifugation. The results of 50 cases examined by the two separation methods are in agreement. However, affinity chromatography is more specific than gel filtration methods and requires a smaller amount of serum. The anti-human IgM globulins, covalently bound to activated Sepharose, maintain their binding capacity to IgM for a long period; and the same gel could be used more than 40 times. Due to its specificity, sensitivity and reliability, affinity chromatography is an efficient tool for routine laboratory diagnosis of a recent primary infection."}
{"id": "PMID:1081413", "title": "[Effect of anti-inflammatory preparations on the process of thermogenesis in blood erythrocytes].", "content": "The authors studied the influence of the main antiphlogistic agents (prednisolone, acetylsalicylic acid, rheopyrine, chloroquine and indomethacine) on the thermal effect of the peripheral blood erythrocytes in man. It was found that only chloroquine possessed a significant inhibitory effect. This fact pointed to the inhibition by chloroquine of energy production in the process of glycolysis, which could be regarded as one of the factors of the therapeutic action of this preparation.", "contents": "[Effect of anti-inflammatory preparations on the process of thermogenesis in blood erythrocytes]. The authors studied the influence of the main antiphlogistic agents (prednisolone, acetylsalicylic acid, rheopyrine, chloroquine and indomethacine) on the thermal effect of the peripheral blood erythrocytes in man. It was found that only chloroquine possessed a significant inhibitory effect. This fact pointed to the inhibition by chloroquine of energy production in the process of glycolysis, which could be regarded as one of the factors of the therapeutic action of this preparation."}
{"id": "PMID:1081414", "title": "Use of logic-based flow patterns in the investigation and management of surgical disorders.", "content": "The principles used in the development of logic-based flow charts for use by computer have been applied to the decision-making processes encountered in surgical disorders. Such charts do not attempt to alter one's decisions, but rather present a more logical approach to a given problem, based on past experience. It has been shown that the use of flow charts may be of material benefit to the patient, as well as having other advantages.", "contents": "Use of logic-based flow patterns in the investigation and management of surgical disorders. The principles used in the development of logic-based flow charts for use by computer have been applied to the decision-making processes encountered in surgical disorders. Such charts do not attempt to alter one's decisions, but rather present a more logical approach to a given problem, based on past experience. It has been shown that the use of flow charts may be of material benefit to the patient, as well as having other advantages."}
{"id": "PMID:1081415", "title": "Meckel's bleeding diverticulum diagnosed by mesenteric arteriography.", "content": "The details of a young adult patient who presented with chronic gastro-intestinal blood loss as a complication of Meckel's diverticulum are reported. A preoperative diagnosis was made using superior mesenteric arteriography. The merits of superior mesenteric arteriography and other investigations currently available in the diagnosis of Meckel's diverticulum are discussed.", "contents": "Meckel's bleeding diverticulum diagnosed by mesenteric arteriography. The details of a young adult patient who presented with chronic gastro-intestinal blood loss as a complication of Meckel's diverticulum are reported. A preoperative diagnosis was made using superior mesenteric arteriography. The merits of superior mesenteric arteriography and other investigations currently available in the diagnosis of Meckel's diverticulum are discussed."}
{"id": "PMID:1081417", "title": "Comparison of fibreoptic endoscopy in acute upper gastrointestinal haemorrhage in Africans and Europeans.", "content": "The results of endoscopy in acute upper gastrointestinal haemorrhage were compared in a group of 138 Africans and one of 84 Europeans. Contrary to widely held clinical opinion, the incidence of gastric and duodenal ulceration was similar in the two races. Peptic ulcers were the main source of bleeding in both groups and were surprisingly more common than varices in the Africans. Bleeding from varices, however, was far more common in the Africans than in the Europeans. Stomal ulcers were confined to Europeans. Gastric erosions, often attributed to herbal medicines, were more common in the Africans but the difference was not significant. The study was not designed to determine reduced mortality since the introduction of endoscopy, but management, especially in the Africans, was aided by early recognition of haemorrhage from oesophageal varices and acute gastric erosions.", "contents": "Comparison of fibreoptic endoscopy in acute upper gastrointestinal haemorrhage in Africans and Europeans. The results of endoscopy in acute upper gastrointestinal haemorrhage were compared in a group of 138 Africans and one of 84 Europeans. Contrary to widely held clinical opinion, the incidence of gastric and duodenal ulceration was similar in the two races. Peptic ulcers were the main source of bleeding in both groups and were surprisingly more common than varices in the Africans. Bleeding from varices, however, was far more common in the Africans than in the Europeans. Stomal ulcers were confined to Europeans. Gastric erosions, often attributed to herbal medicines, were more common in the Africans but the difference was not significant. The study was not designed to determine reduced mortality since the introduction of endoscopy, but management, especially in the Africans, was aided by early recognition of haemorrhage from oesophageal varices and acute gastric erosions."}
{"id": "PMID:1081420", "title": "Isoimmune haemolysis in pathogenesis of anaemia after cardiac surgery.", "content": "A patient who had received multiple transfusions developed antiglobulin-positive haemolytic anaemia due to a delayed haemolytic transfusion reaction. Many cases of haemolytic anaemia after cardiac surgery could be explained on this basis.", "contents": "Isoimmune haemolysis in pathogenesis of anaemia after cardiac surgery. A patient who had received multiple transfusions developed antiglobulin-positive haemolytic anaemia due to a delayed haemolytic transfusion reaction. Many cases of haemolytic anaemia after cardiac surgery could be explained on this basis."}
{"id": "PMID:1081423", "title": "Antitrichomonal antibody in the vaginal secretions of women infected with T. vaginalis.", "content": "Vaginal secretions from 508 women were examined for evidence of infection by Trichomonas vaginalis, and for antibodies directed against this organism; 42 women (8-3 per cent.) were found to be infected. Secretions from 29 of these women were assayed and antibody apparently directed against T. vaginalis was found in 22 (76 per cent.) of them. Eight out of nineteen secretions (42 per cent.) from apparently uninfected women also contained antibody. Amongst the infected women, no correlation could be found between the presence or absence of antibody and the degree of inflammation, duration of symptoms, use of oral contraceptives, or additional sexually-transmitted disease; there was, however, a slight suggestion that low parasite counts in the vaginal secretions were associated with the presence of antitrichomonal antibody.", "contents": "Antitrichomonal antibody in the vaginal secretions of women infected with T. vaginalis. Vaginal secretions from 508 women were examined for evidence of infection by Trichomonas vaginalis, and for antibodies directed against this organism; 42 women (8-3 per cent.) were found to be infected. Secretions from 29 of these women were assayed and antibody apparently directed against T. vaginalis was found in 22 (76 per cent.) of them. Eight out of nineteen secretions (42 per cent.) from apparently uninfected women also contained antibody. Amongst the infected women, no correlation could be found between the presence or absence of antibody and the degree of inflammation, duration of symptoms, use of oral contraceptives, or additional sexually-transmitted disease; there was, however, a slight suggestion that low parasite counts in the vaginal secretions were associated with the presence of antitrichomonal antibody."}
{"id": "PMID:1081424", "title": "Effect of light stimulation on the synthesis and release of GABA in rat and frog retinae.", "content": "The effect of light stimulation on the incorporation of label from [14C]glucose and [3H]acetate into aspartate, glutamate, glutamine and GABA, both in vivo and in vitro, was studied in dark-adapted frog and rat retinae. With [14C]glucose as the precursor, but not with [3H]acetate, flashes of light appeared to increase the in vivo syntheses of both GABA and glutamine at the expense of glutamate in frog retina, while attenuating the labelling of aspartate and glutamate in isolated frog tissues. Amino acid synthesis from these radioactive substrates in rat retina was not altered under these conditions. Also, the turnover rates of amino acids derived from [14C]-glucose in rat retina in vivo were closely similar in light and darkness. Isolated retinae from both species accumulated [14C]GABA from the incubation medium. In each case the subsequent release of this compound was augmented by electric field stimulation, high K+ and by a high concentration of cold GABA in the medium, but not by a flashing light. Similarly, application of a light stimulus was unable to evoke the liberation of [14C]GABA derived from [14C]glucose in either species.", "contents": "Effect of light stimulation on the synthesis and release of GABA in rat and frog retinae. The effect of light stimulation on the incorporation of label from [14C]glucose and [3H]acetate into aspartate, glutamate, glutamine and GABA, both in vivo and in vitro, was studied in dark-adapted frog and rat retinae. With [14C]glucose as the precursor, but not with [3H]acetate, flashes of light appeared to increase the in vivo syntheses of both GABA and glutamine at the expense of glutamate in frog retina, while attenuating the labelling of aspartate and glutamate in isolated frog tissues. Amino acid synthesis from these radioactive substrates in rat retina was not altered under these conditions. Also, the turnover rates of amino acids derived from [14C]-glucose in rat retina in vivo were closely similar in light and darkness. Isolated retinae from both species accumulated [14C]GABA from the incubation medium. In each case the subsequent release of this compound was augmented by electric field stimulation, high K+ and by a high concentration of cold GABA in the medium, but not by a flashing light. Similarly, application of a light stimulus was unable to evoke the liberation of [14C]GABA derived from [14C]glucose in either species."}
{"id": "PMID:1081426", "title": "The effect of fluoride alone or fluoride followed by calcium and vitamin D on disuse osteoporosis of the rat tail vertebrae.", "content": "The therapeutic effect of fluoride alone or fluoride followed by calcium and vitamin D administration on the osteoporosis induced by immobilization of the rat tail vertebrae was investigated. Tail immobilization was carried out by musclectomy and tendectomy. After 60 days of immobilization, the vertebrae were examined for bone mass, breaking strength, ash percentage and Ca, P and F content. NaF or Ca and vitamin D as separate treatments improved the osteoporosis induced by immobilization. The effects of NaF treatment were more pronounced than those of Ca and vitamin D. NaF and NaF followed by Ca and vitamin D produced a similar degree of improvement in breaking strength. The fact that fluoride and calcium and vitamin D were administered separately and sequentially may explain the absence of a synergistic effect.", "contents": "The effect of fluoride alone or fluoride followed by calcium and vitamin D on disuse osteoporosis of the rat tail vertebrae. The therapeutic effect of fluoride alone or fluoride followed by calcium and vitamin D administration on the osteoporosis induced by immobilization of the rat tail vertebrae was investigated. Tail immobilization was carried out by musclectomy and tendectomy. After 60 days of immobilization, the vertebrae were examined for bone mass, breaking strength, ash percentage and Ca, P and F content. NaF or Ca and vitamin D as separate treatments improved the osteoporosis induced by immobilization. The effects of NaF treatment were more pronounced than those of Ca and vitamin D. NaF and NaF followed by Ca and vitamin D produced a similar degree of improvement in breaking strength. The fact that fluoride and calcium and vitamin D were administered separately and sequentially may explain the absence of a synergistic effect."}
{"id": "PMID:1081427", "title": "An efferent effect on a rhythmic response in the frog retina.", "content": "Extracellular recordings made from class III ganglion cells demonstrated a rhythmic bursting firing pattern in response to the offset of a mesopic level stimulus of 15, 30, and 90-s duration. The average bursting frequency was found to be 12.8 +/- 0.3 bursts per second. The bursts of action potentials had a duration of 0.047 +/- 0.002 s and separated by silent periods of 0.028 +/- 0.006 s. When a reversible cold block was applied to the optic chiasma, the rhythmic bursting pattern was replaced with a firing pattern that appeared random, indicating that efferent fibers to the retina might regulate the rhythmic response.", "contents": "An efferent effect on a rhythmic response in the frog retina. Extracellular recordings made from class III ganglion cells demonstrated a rhythmic bursting firing pattern in response to the offset of a mesopic level stimulus of 15, 30, and 90-s duration. The average bursting frequency was found to be 12.8 +/- 0.3 bursts per second. The bursts of action potentials had a duration of 0.047 +/- 0.002 s and separated by silent periods of 0.028 +/- 0.006 s. When a reversible cold block was applied to the optic chiasma, the rhythmic bursting pattern was replaced with a firing pattern that appeared random, indicating that efferent fibers to the retina might regulate the rhythmic response."}
{"id": "PMID:1081428", "title": "Bactericidal substance produced by Haemophilus influenzae b.", "content": "During bacteriophage studies on Haemophilus influenzer, it was observed that encapsulated type b and unencapsulated Rb strains released a bactericidal substance acitve against types a, c, d, e, and f H. influenzae, non-typable H. influenzae strains, other Haemophilus species, and certain members of the Enterobacteriaceae. The bactericidal activity was assayed by a plaque test utilizing an Rd strain as an indicator lawn and was also demonstrated in mixed broth cultures of a producer strain and an indicator strain. Immediately lysis of sensitive bacteria by the factor was not evident. The factor is sensitive to trypsin but resistant to deoxyribonuclease, treatment with 2-mercaptoethanol, lipase, alpha-amylase, and heating in a 100 degrees C water bath for 20 min. The activity is not dependent upon increased Ca2+ or Mg2+ concentration as is necessary for HP1C1 and S2 phage propagation. The bactericidal factor is not pelleted by high-speed centrifugation at 150,000  X g for 6 h. Treatment with ultraviolet light or mitomycin C does not result in observable phage, phage-like particles, or increased bactericidal activity. T-HE BACTERICIDAL FACTOR IS NOT A TYPICAL SMALL MOLECULAR WEIGHT \"COLICIN-LIKE\" BACTERiocin in that it is not inducible, has a wider range of activity, and does not kill by \"single-hit\" kinetics. On preliminary characterization, it is a thermostable protein toxic to certain bacterial strains.", "contents": "Bactericidal substance produced by Haemophilus influenzae b. During bacteriophage studies on Haemophilus influenzer, it was observed that encapsulated type b and unencapsulated Rb strains released a bactericidal substance acitve against types a, c, d, e, and f H. influenzae, non-typable H. influenzae strains, other Haemophilus species, and certain members of the Enterobacteriaceae. The bactericidal activity was assayed by a plaque test utilizing an Rd strain as an indicator lawn and was also demonstrated in mixed broth cultures of a producer strain and an indicator strain. Immediately lysis of sensitive bacteria by the factor was not evident. The factor is sensitive to trypsin but resistant to deoxyribonuclease, treatment with 2-mercaptoethanol, lipase, alpha-amylase, and heating in a 100 degrees C water bath for 20 min. The activity is not dependent upon increased Ca2+ or Mg2+ concentration as is necessary for HP1C1 and S2 phage propagation. The bactericidal factor is not pelleted by high-speed centrifugation at 150,000  X g for 6 h. Treatment with ultraviolet light or mitomycin C does not result in observable phage, phage-like particles, or increased bactericidal activity. T-HE BACTERICIDAL FACTOR IS NOT A TYPICAL SMALL MOLECULAR WEIGHT \"COLICIN-LIKE\" BACTERiocin in that it is not inducible, has a wider range of activity, and does not kill by \"single-hit\" kinetics. On preliminary characterization, it is a thermostable protein toxic to certain bacterial strains."}
{"id": "PMID:1081429", "title": "Changes in the mitogen response of lymphoid cells with progressive tumor growth.", "content": "The response of spleen and lymph node cells from tumor-bearing animals to a variety of mitogens was measured. It was found that progressive tumor growth diminished the reactivity of spleen cells to the mitogen phytohemagglutinin. Lymph node cells from the same animal were not consistently so affected. The reactivity of spleen cells from tumor-bearing donors to concanavalin A was somewhat depressed, while stimulation by bone marrow-derived lymphocyte mitogens such as endotoxin and pokeweed mitogen was in some cases enhanced. Theta antigen expression in tumor bearer spleens was found to decline steadily after 7 days of tumor growth. Cytological analysis revealed that the normal structure of the spleens of tumor animals was infiltrated by myeloid elements. The changes described occurred regardless of whether the tumors were chemically or virally induced. Excision of tumors after long periods of growth resulted in prompt return of splenic phytohemagglutinin sensitivity. The data suggests that loss or incapacitation of parts of the normal thymus-derived lymphocyte components may occur in the spleens of animals with progressive neoplastic growth.", "contents": "Changes in the mitogen response of lymphoid cells with progressive tumor growth. The response of spleen and lymph node cells from tumor-bearing animals to a variety of mitogens was measured. It was found that progressive tumor growth diminished the reactivity of spleen cells to the mitogen phytohemagglutinin. Lymph node cells from the same animal were not consistently so affected. The reactivity of spleen cells from tumor-bearing donors to concanavalin A was somewhat depressed, while stimulation by bone marrow-derived lymphocyte mitogens such as endotoxin and pokeweed mitogen was in some cases enhanced. Theta antigen expression in tumor bearer spleens was found to decline steadily after 7 days of tumor growth. Cytological analysis revealed that the normal structure of the spleens of tumor animals was infiltrated by myeloid elements. The changes described occurred regardless of whether the tumors were chemically or virally induced. Excision of tumors after long periods of growth resulted in prompt return of splenic phytohemagglutinin sensitivity. The data suggests that loss or incapacitation of parts of the normal thymus-derived lymphocyte components may occur in the spleens of animals with progressive neoplastic growth."}
{"id": "PMID:1081431", "title": "Serum levels of colony stimulating factor(s) and the growth of bone marrow cells in diffusion chambers.", "content": "Fluctuations in the body fluids of long-ranged humoral substance(s) capable of stimulating the growth of bone marrow granulocytic and macrophage-like cells in diffusion chamber cultures in vivo, was observed after whole body irradiation of mice. The fluctuation pattern was similar to that of the in vitro colony stimulating factor(s) of the sera of irradiated mice which indicates a relation between in vivo and in vitro active factor(s).", "contents": "Serum levels of colony stimulating factor(s) and the growth of bone marrow cells in diffusion chambers. Fluctuations in the body fluids of long-ranged humoral substance(s) capable of stimulating the growth of bone marrow granulocytic and macrophage-like cells in diffusion chamber cultures in vivo, was observed after whole body irradiation of mice. The fluctuation pattern was similar to that of the in vitro colony stimulating factor(s) of the sera of irradiated mice which indicates a relation between in vivo and in vitro active factor(s)."}
{"id": "PMID:1081432", "title": "The presence of a CSF enhancing activity in the serum of endotoxin-treated mice.", "content": "Serum taken from mice a few hours after injection of endotoxin is a potent source of a stimulator of in vitro myelopoiesis. By means of dose-response studies, the biological activity of this material was compared to that of a colony stimulating factor (CSF) from pregnant mouse uteri. Postendotoxin serum appears to contain two different activities: a stimulating activity which may be identical to CSF and an activity which augments the action of CSF. The separate nature of the two activities is demonstrated by differences in the rate at which they are diluted out and by differences in the time at which they are maximally present after indotoxin administration. It is therefore concluded that the colony-stimulating properties of postendotoxin serum are not due solely to CSF present in the serum.", "contents": "The presence of a CSF enhancing activity in the serum of endotoxin-treated mice. Serum taken from mice a few hours after injection of endotoxin is a potent source of a stimulator of in vitro myelopoiesis. By means of dose-response studies, the biological activity of this material was compared to that of a colony stimulating factor (CSF) from pregnant mouse uteri. Postendotoxin serum appears to contain two different activities: a stimulating activity which may be identical to CSF and an activity which augments the action of CSF. The separate nature of the two activities is demonstrated by differences in the rate at which they are diluted out and by differences in the time at which they are maximally present after indotoxin administration. It is therefore concluded that the colony-stimulating properties of postendotoxin serum are not due solely to CSF present in the serum."}
{"id": "PMID:1081433", "title": "Ultrastructure of the stellate cell in the pars intermedia of the frog, Rana pipiens.", "content": "Stellate cells in the pars intermedia of adult Rana pipiens black-and-white background adapted for 14 days were studied by electron microscopy. This cell type forms a capsule around the secretory cells at the surface of the gland; it also lines the perivascular spaces between the pars intermedia and pars nervosa. The cells are characterized by the lack of well-developed secretory organelles and by the presence of long procesees which extend into the intercellular spaces of the secretory cells and reach the surface of the gland and the perivascular spaces. Occasional desmosomes link the stellate cells to adjacent cells; bundles of microfilaments occur frequently. Microtubules run parallel to the long axis of the cell processes. In light of the possibility that the stellate cells might be involved in transfer of substances to and from the melanophore-stimulating hormone (MSH) secretory cells, they were studied for indications of membrane activity. The stellate cell plasma membranes abutting the pericapillary spaces showed intense micropinocytotic vesicle activity. Also at the gland surface the electron-lucent vesicles were common at the plasma membranes of the stellate cells. Along the plasma membranes of the stellate cell processes extending between the secretory cells were vesicles which were somewhat electron-dense. This membrane activity would suggest an active role for these cells in addition to a sustentacular role.", "contents": "Ultrastructure of the stellate cell in the pars intermedia of the frog, Rana pipiens. Stellate cells in the pars intermedia of adult Rana pipiens black-and-white background adapted for 14 days were studied by electron microscopy. This cell type forms a capsule around the secretory cells at the surface of the gland; it also lines the perivascular spaces between the pars intermedia and pars nervosa. The cells are characterized by the lack of well-developed secretory organelles and by the presence of long procesees which extend into the intercellular spaces of the secretory cells and reach the surface of the gland and the perivascular spaces. Occasional desmosomes link the stellate cells to adjacent cells; bundles of microfilaments occur frequently. Microtubules run parallel to the long axis of the cell processes. In light of the possibility that the stellate cells might be involved in transfer of substances to and from the melanophore-stimulating hormone (MSH) secretory cells, they were studied for indications of membrane activity. The stellate cell plasma membranes abutting the pericapillary spaces showed intense micropinocytotic vesicle activity. Also at the gland surface the electron-lucent vesicles were common at the plasma membranes of the stellate cells. Along the plasma membranes of the stellate cell processes extending between the secretory cells were vesicles which were somewhat electron-dense. This membrane activity would suggest an active role for these cells in addition to a sustentacular role."}
{"id": "PMID:1081436", "title": "Potassium influx in the frog atrium during the cardiac cycle.", "content": "A method for measuring inwardly directed transmembrane tracer flow during the cardiac cycle was developed and applied to a study of 42K influx in frog atrial trabeculae. A fine frog atrial fiber was suspended in a stream of nonisotopic perfusate into which a smaller tracer-containing bolus could be injected, subjecting the fiber to a brief, controlled exposure to the tracer at any desired point in the cardiac cycle. In an experiment, the tissue was exposed to a fixed number of radioactive pulses at a selected point in the cardiac cycle; a brief flush with nonradioactive perfusate removed ambient and extracellular label and an extended wash and removed the remaining intracellular tracer for radioassay. The same procedure was repeated at different points in the cycle, and the resulting tracer uptake at each point measured the relative influx of the particular ion. In this way, a characteristic and reproducible 42K influx profile was demonstrated which exhibited a marked drop below diastolic values during the first 500 msec or so of the action potential followed by a rise and an overshoot above resting values. The time of return to the resting level was somewhat uncertain but was tentatively placed in the vicinity of rapid repolarization. We suggest that the rise and the overshoot reflect the activity of the membrane Na+, K+-adenosinetriphosphatase.", "contents": "Potassium influx in the frog atrium during the cardiac cycle. A method for measuring inwardly directed transmembrane tracer flow during the cardiac cycle was developed and applied to a study of 42K influx in frog atrial trabeculae. A fine frog atrial fiber was suspended in a stream of nonisotopic perfusate into which a smaller tracer-containing bolus could be injected, subjecting the fiber to a brief, controlled exposure to the tracer at any desired point in the cardiac cycle. In an experiment, the tissue was exposed to a fixed number of radioactive pulses at a selected point in the cardiac cycle; a brief flush with nonradioactive perfusate removed ambient and extracellular label and an extended wash and removed the remaining intracellular tracer for radioassay. The same procedure was repeated at different points in the cycle, and the resulting tracer uptake at each point measured the relative influx of the particular ion. In this way, a characteristic and reproducible 42K influx profile was demonstrated which exhibited a marked drop below diastolic values during the first 500 msec or so of the action potential followed by a rise and an overshoot above resting values. The time of return to the resting level was somewhat uncertain but was tentatively placed in the vicinity of rapid repolarization. We suggest that the rise and the overshoot reflect the activity of the membrane Na+, K+-adenosinetriphosphatase."}
{"id": "PMID:1081440", "title": "The lymphocyte response in Hodgkin's disease.", "content": "Fifty cases of Hodgkin's disease have been investigated using the lymphocyte transformation response to phytohaemagglutinin (PHA) as a mesure of their immunological reaction. The average response of all the cases was lower than that of the controls and lower than that of cases of carcinoma from all sites. Cases of Hodgkin's disease which had been treated previously with radiotherapy and/or chemotherapy had a markedly lower response than that of the untreated cases. There was no definite correlation between the stage of the disease and the lymphocyte response with PHA. Seven cases were also tested with pokeweek mitogen (PWM) and the response of the lymphocytes to PWM was found to parallel that of the PHA.", "contents": "The lymphocyte response in Hodgkin's disease. Fifty cases of Hodgkin's disease have been investigated using the lymphocyte transformation response to phytohaemagglutinin (PHA) as a mesure of their immunological reaction. The average response of all the cases was lower than that of the controls and lower than that of cases of carcinoma from all sites. Cases of Hodgkin's disease which had been treated previously with radiotherapy and/or chemotherapy had a markedly lower response than that of the untreated cases. There was no definite correlation between the stage of the disease and the lymphocyte response with PHA. Seven cases were also tested with pokeweek mitogen (PWM) and the response of the lymphocytes to PWM was found to parallel that of the PHA."}
{"id": "PMID:1081441", "title": "The twenty-four hour pneumoencephalogram: with particular reference to ventricular size a series of 150 patients and a review of the literature.", "content": "A series of 150 pneumoencephalograms which included 24 h films was reviewed to evaluate previous contradictory reports concerning changes in ventricular size at 24 h and to establish whether it is useful to obtain these follow-up films. In normal patients the changes in the ventricular index one day following the pneumoencephalogram ranged between -4-1 to +6-4% of the initial index. Statistically significant dilatation of the frontal horns of the lateral ventricles was observed in both patients who had dementia and central atrophy, and in those with normal pressure hydrocephalus. This ventricular enlargement was associated with deterioration in neurological status in some cases. Since ventricular enlargement at 24 h occurs in patients both with normal pressure hydrocephalus and with cerebral atrophy with hydrocephalus, it cannot be used to differentiate between these conditions although the most marked changes have been seen in the former. Significant dilatation did not occur in patients without intracranial disease, with cortical atrophy without hydrocephalus, or in a miscellaneous group. In patients with a history of trauma, enlargement at 24 h occurred only when there was some other encephalographic abnormality. The changes in size of the ventricles were not related to the age of the patient, the volume of the injected air, or to the presnece of subdural air. There were other findings on the follow=up radiographs. Cortical atrophy was more clearly demonstrated than on the filling films. Dilatation of the temporal horns, of questionable diagnostic significance, was not infrequently observed 24 h after air injection. The mechanisms of these changes are discussed; no single theory provides a complete explanation.", "contents": "The twenty-four hour pneumoencephalogram: with particular reference to ventricular size a series of 150 patients and a review of the literature. A series of 150 pneumoencephalograms which included 24 h films was reviewed to evaluate previous contradictory reports concerning changes in ventricular size at 24 h and to establish whether it is useful to obtain these follow-up films. In normal patients the changes in the ventricular index one day following the pneumoencephalogram ranged between -4-1 to +6-4% of the initial index. Statistically significant dilatation of the frontal horns of the lateral ventricles was observed in both patients who had dementia and central atrophy, and in those with normal pressure hydrocephalus. This ventricular enlargement was associated with deterioration in neurological status in some cases. Since ventricular enlargement at 24 h occurs in patients both with normal pressure hydrocephalus and with cerebral atrophy with hydrocephalus, it cannot be used to differentiate between these conditions although the most marked changes have been seen in the former. Significant dilatation did not occur in patients without intracranial disease, with cortical atrophy without hydrocephalus, or in a miscellaneous group. In patients with a history of trauma, enlargement at 24 h occurred only when there was some other encephalographic abnormality. The changes in size of the ventricles were not related to the age of the patient, the volume of the injected air, or to the presnece of subdural air. There were other findings on the follow=up radiographs. Cortical atrophy was more clearly demonstrated than on the filling films. Dilatation of the temporal horns, of questionable diagnostic significance, was not infrequently observed 24 h after air injection. The mechanisms of these changes are discussed; no single theory provides a complete explanation."}
{"id": "PMID:1081442", "title": "Prevalance of malocclusion in young Finnish Skolt-Lapps.", "content": "Two hundred Skolt Lapps aged 8 to 16 years were examined for malocclusion with the aid of intraoral radiography and dental casts. The frequency of loss of permanent teeth was high, particularly of the first molars. Also, many teeth were severely defective because of caries. Hypodontia was very common. The frequency of tipped and rotated teeth was high, probably as a consequence of the frequent loss of neighboring teeth. Space and occlusal anomalies were roughly as common as in urbanized Swedish and Danish materials. The need for orthodontic treatment, judged on a 5-grade scale by one examiner, was found to be greater than in Swedish materials.", "contents": "Prevalance of malocclusion in young Finnish Skolt-Lapps. Two hundred Skolt Lapps aged 8 to 16 years were examined for malocclusion with the aid of intraoral radiography and dental casts. The frequency of loss of permanent teeth was high, particularly of the first molars. Also, many teeth were severely defective because of caries. Hypodontia was very common. The frequency of tipped and rotated teeth was high, probably as a consequence of the frequent loss of neighboring teeth. Space and occlusal anomalies were roughly as common as in urbanized Swedish and Danish materials. The need for orthodontic treatment, judged on a 5-grade scale by one examiner, was found to be greater than in Swedish materials."}
{"id": "PMID:1081438", "title": "The potential for an improved prognosis with chemotherapy in osteogenic sarcoma.", "content": "Effective chemotherapeutic agents administered shortly after definitive primary treatment to patients with osteogenic sarcoma have altered the natural history of the disease. In contrast to past experience, pulmonary metastases fail to develop in the overwhelming majority of patients receiving treatment with chemotherapy. Patients with established pulmonary metastases have also been salvaged. The therapeutic strategy advocated for different categories of patients is outlined. Although chemotherapy involves additional hospitalization and inconvenience, this additional mode of treatment appears to be the only mechanism by which a substantial improvement in survival of osteogenic sarcoma can presently be achieved.", "contents": "The potential for an improved prognosis with chemotherapy in osteogenic sarcoma. Effective chemotherapeutic agents administered shortly after definitive primary treatment to patients with osteogenic sarcoma have altered the natural history of the disease. In contrast to past experience, pulmonary metastases fail to develop in the overwhelming majority of patients receiving treatment with chemotherapy. Patients with established pulmonary metastases have also been salvaged. The therapeutic strategy advocated for different categories of patients is outlined. Although chemotherapy involves additional hospitalization and inconvenience, this additional mode of treatment appears to be the only mechanism by which a substantial improvement in survival of osteogenic sarcoma can presently be achieved."}
{"id": "PMID:1081443", "title": "The comparative evaluation of methoxyflurane and diethyl ether action upon an active sodium transport across isolated frog skin preparation.", "content": "The experiments were performed in vitro using the frog skin preparations and Ussing method. The effect of methoxyflurane was investigated in 18 sections and that of diethyl ether in 16 sections. Both the anaesthetics were found to affect the frog skin preparation in qualitatively similar way. They both demonstrated a two-phase character of action. Decrease in active sodium transport is preceeded by a transient increase in bioelectric indices of the membrane. The effect was reversible after removing of the drugs from the medium. In order to determine the mechanism of action of the two anaesthetics they were given in combination with ADH and insulin. It was found that the mechanism of action of both the drugs is not analogical to any of the hormones examined, at least, as far as the first phase of action is concerned.", "contents": "The comparative evaluation of methoxyflurane and diethyl ether action upon an active sodium transport across isolated frog skin preparation. The experiments were performed in vitro using the frog skin preparations and Ussing method. The effect of methoxyflurane was investigated in 18 sections and that of diethyl ether in 16 sections. Both the anaesthetics were found to affect the frog skin preparation in qualitatively similar way. They both demonstrated a two-phase character of action. Decrease in active sodium transport is preceeded by a transient increase in bioelectric indices of the membrane. The effect was reversible after removing of the drugs from the medium. In order to determine the mechanism of action of the two anaesthetics they were given in combination with ADH and insulin. It was found that the mechanism of action of both the drugs is not analogical to any of the hormones examined, at least, as far as the first phase of action is concerned."}
{"id": "PMID:1081444", "title": "Seasonal differences in drug- and temperature-dependent changes of the sodium transport across the isolated frog skin.", "content": "In two series of experiments, one conducted in the spring (May-June) and the other in the fall (September-November), the influence of raising temperature of the medium and of vasopressin, insulin and ethacrynic acid on polarized sodium transport in the epithelium of frog skin in vitro was studied. Net sodium transport was determined electrometrically, and active and passive components of transport by the isotope method. Raising temperature markedly increased both components, as well as net flux and electric potential of the membrane in the spring months, but not in the fall. Regardless of the character and extent of the effect on transport, raising temperature of the medium had no qualitative or quantitative influence on the action of vasopressin, insulin or ethacrynic acid.", "contents": "Seasonal differences in drug- and temperature-dependent changes of the sodium transport across the isolated frog skin. In two series of experiments, one conducted in the spring (May-June) and the other in the fall (September-November), the influence of raising temperature of the medium and of vasopressin, insulin and ethacrynic acid on polarized sodium transport in the epithelium of frog skin in vitro was studied. Net sodium transport was determined electrometrically, and active and passive components of transport by the isotope method. Raising temperature markedly increased both components, as well as net flux and electric potential of the membrane in the spring months, but not in the fall. Regardless of the character and extent of the effect on transport, raising temperature of the medium had no qualitative or quantitative influence on the action of vasopressin, insulin or ethacrynic acid."}
{"id": "PMID:1081446", "title": "Treatment of severe coronary artery disease with quadruple and quintuple saphenous vein grafts. Review of 100 consecutive patients.", "content": "In order to establish the anatomic criteria, the functional results, and the safety of complete myocardial revascularization for severe coronary artery disease, 100 consecutive patients who received four or five saphenous-vein grafts were analyzed. Ages ranged from 37 to 75 years (mean, 56 years). Men predominated by a ratio of 12:1. As an indication of the severity of multiple-vessel disease, 28 percent were in functional class 4, and left ventricular function was classified as good in 47 percent, as fair in 44 percent, and as poor in 8 percent. Coronary arterial scores ranged from 9 to 15 (average, 12.2). Fourteen patients had significant left main coronary arterial obstruction. All 100 patients had grafts to the left anterior descending coronary artery; 96 to the right coronary artery; 94 to the obtuse marginal branch of the circumflex; 78 to a diagonal branch of the left anterior descending; and 27 to the distal circumflex. Operative mortality was 5 percent. Nonfatal perioperative myocardial infarction occurred in 10 percent, and only one of these had low cardiac output. Follow-up from 5 to 23 months showed 95 percent of the patients to be improved, with 70 percent free of angina. Two late deaths occurred, for an overall mortality of 7 percent.", "contents": "Treatment of severe coronary artery disease with quadruple and quintuple saphenous vein grafts. Review of 100 consecutive patients. In order to establish the anatomic criteria, the functional results, and the safety of complete myocardial revascularization for severe coronary artery disease, 100 consecutive patients who received four or five saphenous-vein grafts were analyzed. Ages ranged from 37 to 75 years (mean, 56 years). Men predominated by a ratio of 12:1. As an indication of the severity of multiple-vessel disease, 28 percent were in functional class 4, and left ventricular function was classified as good in 47 percent, as fair in 44 percent, and as poor in 8 percent. Coronary arterial scores ranged from 9 to 15 (average, 12.2). Fourteen patients had significant left main coronary arterial obstruction. All 100 patients had grafts to the left anterior descending coronary artery; 96 to the right coronary artery; 94 to the obtuse marginal branch of the circumflex; 78 to a diagonal branch of the left anterior descending; and 27 to the distal circumflex. Operative mortality was 5 percent. Nonfatal perioperative myocardial infarction occurred in 10 percent, and only one of these had low cardiac output. Follow-up from 5 to 23 months showed 95 percent of the patients to be improved, with 70 percent free of angina. Two late deaths occurred, for an overall mortality of 7 percent."}
{"id": "PMID:1081447", "title": "The experimental and clinical use of intravitreal antibiotics to treat bacterial and fungal endophthalmitis.", "content": "The experimental data involving the use of 14 antibacterial and two antifungal agents have been used to establish a rationale for the management of human endophthalmitis. Twelve cases of endophthalmitis treated under our supervision have been described in detail. The eventual visual outcome is related to two factors: (1) time interval between diagnosis and institution of adequate therapy, and (2) the severity of the organism and its ability to produce exotoxins and proteolytic enzymes. Eleven of 12 cases were cured with the recommended dosages of gentamicin and dexamethasone (400 mcg and 360 mcg, respectively). Eight of the 12 patients retained useful vision of 20/200 or better.", "contents": "The experimental and clinical use of intravitreal antibiotics to treat bacterial and fungal endophthalmitis. The experimental data involving the use of 14 antibacterial and two antifungal agents have been used to establish a rationale for the management of human endophthalmitis. Twelve cases of endophthalmitis treated under our supervision have been described in detail. The eventual visual outcome is related to two factors: (1) time interval between diagnosis and institution of adequate therapy, and (2) the severity of the organism and its ability to produce exotoxins and proteolytic enzymes. Eleven of 12 cases were cured with the recommended dosages of gentamicin and dexamethasone (400 mcg and 360 mcg, respectively). Eight of the 12 patients retained useful vision of 20/200 or better."}
{"id": "PMID:1081449", "title": "[Fatal course of generalized BCG histiocytosis in congenital immune deficiency (author's transl)].", "content": "Severe combined X-linked immune deficiency ended fatally in a six-month-old male infant after generalized BCG infection. Damage in the cell-bound thymus-dependent immune system is decisive in the development of \"BCG histiocytosis\". As a result of absent immunocompetent sensitized T-lymphocytes there is insufficient information and stimulation of macrophages to kill the micro-organsims. The disorder of bactericidal properties of macrophages results in an overwhelming infection with BCG bacteria with a characteristic morphological picture.", "contents": "[Fatal course of generalized BCG histiocytosis in congenital immune deficiency (author's transl)]. Severe combined X-linked immune deficiency ended fatally in a six-month-old male infant after generalized BCG infection. Damage in the cell-bound thymus-dependent immune system is decisive in the development of \"BCG histiocytosis\". As a result of absent immunocompetent sensitized T-lymphocytes there is insufficient information and stimulation of macrophages to kill the micro-organsims. The disorder of bactericidal properties of macrophages results in an overwhelming infection with BCG bacteria with a characteristic morphological picture."}
{"id": "PMID:1081450", "title": "Binding by thyroid hormones by nuclei of cells from bullfrog tadpole tail fins.", "content": "The regression of the tadpole tail is under the direct control of the thyroid hormones and offers a unique system for the study of the action of these hormones. We have examined the binding of L-triiodothyronine (T3) and L-thyroxine (T4) in vitro using tail fin bricks which included epidermal and connective cells. The binding of 125I-labeled hormones was followed in both nuclear and extranuclear fractions. High affinity and limited capacity sites for T3 and T4 were observed only for the nuclear fraction. Scatchard plots gave similar apparent dissociation constants for both hormones, about 10(-10)M. The maximum number of binding sites per nucleus for T3 was about 1500 and for T4 about 800. There was no significant change in the chemical identity of [125I]T3 and [125I]T4 which was associated with binding in the nuclei. Nuclear binding of [125I]T4 was inhibited competitively for both unlabeled T3 and T4, but unlabeled T3 displaced [125I]T3 significantly more than unlabeled T4. Thus, both binding and competition data support the conclusion that tadpole tail nuclei had more T3 than T4 binding sites.", "contents": "Binding by thyroid hormones by nuclei of cells from bullfrog tadpole tail fins. The regression of the tadpole tail is under the direct control of the thyroid hormones and offers a unique system for the study of the action of these hormones. We have examined the binding of L-triiodothyronine (T3) and L-thyroxine (T4) in vitro using tail fin bricks which included epidermal and connective cells. The binding of 125I-labeled hormones was followed in both nuclear and extranuclear fractions. High affinity and limited capacity sites for T3 and T4 were observed only for the nuclear fraction. Scatchard plots gave similar apparent dissociation constants for both hormones, about 10(-10)M. The maximum number of binding sites per nucleus for T3 was about 1500 and for T4 about 800. There was no significant change in the chemical identity of [125I]T3 and [125I]T4 which was associated with binding in the nuclei. Nuclear binding of [125I]T4 was inhibited competitively for both unlabeled T3 and T4, but unlabeled T3 displaced [125I]T3 significantly more than unlabeled T4. Thus, both binding and competition data support the conclusion that tadpole tail nuclei had more T3 than T4 binding sites."}
{"id": "PMID:1081451", "title": "Binding of thyroxine and triiodothyronine by nuclei of isolated tadpole liver cells.", "content": "The binding of L-triiodothyronine (T3) and L-thyroxine (T4) to cytoplasm and nuclei has been studied in isolated Rana catesbeiana tadpole liver cells. Nuclear binding for both thyroid hormones occurred more slowly at 4 C than at 25 C, but reached the same level as at 25 S. Scatchard analyses suggest high affinity, saturable binding sites for both hormones in the nuclear but not in the cytoplasmic fraction. Apparent equilibrium dissociation constants were 6.8 X 10(-10)M and 4.6 X 10(-10)M for T3 and T4, respectively. The maximum number of binding sites per nucleus for T3 was about 12,300 and for T4 about 2,300. Unlabeled T3 competed for the binding of [125I]T3 to nuclei more effectively than unlabeled T4. No difference in the competition of [125I]T4 binding with non-radioactive T3 or T4 was found. Chromatographic analysis of the bound nuclear radioactivity demonstrated no chemical modification for either hormone.", "contents": "Binding of thyroxine and triiodothyronine by nuclei of isolated tadpole liver cells. The binding of L-triiodothyronine (T3) and L-thyroxine (T4) to cytoplasm and nuclei has been studied in isolated Rana catesbeiana tadpole liver cells. Nuclear binding for both thyroid hormones occurred more slowly at 4 C than at 25 C, but reached the same level as at 25 S. Scatchard analyses suggest high affinity, saturable binding sites for both hormones in the nuclear but not in the cytoplasmic fraction. Apparent equilibrium dissociation constants were 6.8 X 10(-10)M and 4.6 X 10(-10)M for T3 and T4, respectively. The maximum number of binding sites per nucleus for T3 was about 12,300 and for T4 about 2,300. Unlabeled T3 competed for the binding of [125I]T3 to nuclei more effectively than unlabeled T4. No difference in the competition of [125I]T4 binding with non-radioactive T3 or T4 was found. Chromatographic analysis of the bound nuclear radioactivity demonstrated no chemical modification for either hormone."}
{"id": "PMID:1081452", "title": "Transport of amino acids in membrane vesicles of Rhodopseudomonas spheroides energized by respiratory and cyclic electron flow.", "content": "Active transport of amino acids in whole cells and membrane vesicles from the facultative photo-synthetic bacterium Rhodopseduomonas spheroides is coupled to electron flow in the respiratory chain and in the cyclic electron transfer system. In vesicles made from cells grown aerobically in the dark transport of amino acids is most effectively energized by the oxidation of NADH and to a lesser extent by ascorbate or succinate in the presence of N,N,N',N'-tetramethyl-1, 4-phenyldiamine dihydrochloride or by ascorbate + phenazine methosulphate via the respiratory chain with oxygen as terminal electron acceptor. In membrane vesicles from cells grown anaerobically in the light the energy for active transport of amino acids is supplied upon illumination by photo-oxidation of bacteriochlorophyll and subsequent electron flow through the cyclic electron transfer system. The initial rate of transport increases with the light intensity upto saturation levels. In both vesicle preparations, inhibitors of electron transfer flow inhibit amino acid uptake. In order to obtain light-driven amino acid transport the isolation of membrane vesicles needs to be performed in a medium with a redox potential between 0 and 100 mV. Illumination of vesicles made from cells grown anaerobically in the light results in the generation of a membrane potential as is indicated by the uptake of the lipophylic cation triphenyl-methylphosphonium.", "contents": "Transport of amino acids in membrane vesicles of Rhodopseudomonas spheroides energized by respiratory and cyclic electron flow. Active transport of amino acids in whole cells and membrane vesicles from the facultative photo-synthetic bacterium Rhodopseduomonas spheroides is coupled to electron flow in the respiratory chain and in the cyclic electron transfer system. In vesicles made from cells grown aerobically in the dark transport of amino acids is most effectively energized by the oxidation of NADH and to a lesser extent by ascorbate or succinate in the presence of N,N,N',N'-tetramethyl-1, 4-phenyldiamine dihydrochloride or by ascorbate + phenazine methosulphate via the respiratory chain with oxygen as terminal electron acceptor. In membrane vesicles from cells grown anaerobically in the light the energy for active transport of amino acids is supplied upon illumination by photo-oxidation of bacteriochlorophyll and subsequent electron flow through the cyclic electron transfer system. The initial rate of transport increases with the light intensity upto saturation levels. In both vesicle preparations, inhibitors of electron transfer flow inhibit amino acid uptake. In order to obtain light-driven amino acid transport the isolation of membrane vesicles needs to be performed in a medium with a redox potential between 0 and 100 mV. Illumination of vesicles made from cells grown anaerobically in the light results in the generation of a membrane potential as is indicated by the uptake of the lipophylic cation triphenyl-methylphosphonium."}
{"id": "PMID:1081455", "title": "Amplification of colony stimulating activity in human serum by interaction with CSA from other sources.", "content": "An enhancing interaction of a human serum factor with other sources of colony stimulating activity (CSA) is described. Using a mouse colony forming cell assay, the enhancing effect of human serum was observed with suboptimal doses of colony stimulating material from monocytic cell feeder layers, supernatant from peripheral monocytic cells, and L cell supernatant. The enhancing factor is heat stable, is not dialyzable and is not extractable by ether. The enhancing phenomenon does not require the presence of viable cells in the feeder layers. The observation of enhancing interaction makes interpretation of all data from CSA stimulation experiments more complex than previously appreciated.", "contents": "Amplification of colony stimulating activity in human serum by interaction with CSA from other sources. An enhancing interaction of a human serum factor with other sources of colony stimulating activity (CSA) is described. Using a mouse colony forming cell assay, the enhancing effect of human serum was observed with suboptimal doses of colony stimulating material from monocytic cell feeder layers, supernatant from peripheral monocytic cells, and L cell supernatant. The enhancing factor is heat stable, is not dialyzable and is not extractable by ether. The enhancing phenomenon does not require the presence of viable cells in the feeder layers. The observation of enhancing interaction makes interpretation of all data from CSA stimulation experiments more complex than previously appreciated."}
{"id": "PMID:1081456", "title": "Colony stimulating factor and the regulation of granulopoiesis and macrophage production.", "content": "The growth and differentiation of  colonies of granulocytes and/or macrophages in semisolid cultures of hemopoietic cells requires the presence of \"colony stimulating\" factors (CS factors). Colony stimulating factors are found in serum, urine and certain tissues, and in media \"conditioned\" by certain cells in culture. A procedure has been developed for purification of CS factor from human urine. The 100,000-fold purified product is active on mouse bone marrow cells at less than or equal to 10(-11) M but is only partially purified. Polydispersity of activity has made further purification difficult and may necessitate use of biologically selective techniques such as immunosorbent chromatography. Knowledge of the properties of human urinary CS factor has been derived from the in vitro assay of biological activity following various treatments. It appears to be a sialic acid-containing glycoprotein of molecular weight 45,000-60,000 daltons that migrates electrophoretically between alpha1 globulin and albumin. Highly purified CS factor preparations can be iodinated with five atoms of iodine per molecule of protein without loss of biological activity. Neutralizing antibody to human urinary CS factor neutralizes CS factor from other human and monkey sources. Higher concentrations of antiserum are required for neutralization of murine CS factor. Human urinary CS factor has similar properties to human and murine CS factors that stimulate the early appearance of macrophages in murine colonies. It is physically distinct from CS factors that stimulate the formation of pure granulocytic colonies by murine or human hemopoietic cells. Purified CS factor of the human urinary type is being used to investigate the nature of the interaction of CS factor with target cells and its physiological role in vivo.", "contents": "Colony stimulating factor and the regulation of granulopoiesis and macrophage production. The growth and differentiation of  colonies of granulocytes and/or macrophages in semisolid cultures of hemopoietic cells requires the presence of \"colony stimulating\" factors (CS factors). Colony stimulating factors are found in serum, urine and certain tissues, and in media \"conditioned\" by certain cells in culture. A procedure has been developed for purification of CS factor from human urine. The 100,000-fold purified product is active on mouse bone marrow cells at less than or equal to 10(-11) M but is only partially purified. Polydispersity of activity has made further purification difficult and may necessitate use of biologically selective techniques such as immunosorbent chromatography. Knowledge of the properties of human urinary CS factor has been derived from the in vitro assay of biological activity following various treatments. It appears to be a sialic acid-containing glycoprotein of molecular weight 45,000-60,000 daltons that migrates electrophoretically between alpha1 globulin and albumin. Highly purified CS factor preparations can be iodinated with five atoms of iodine per molecule of protein without loss of biological activity. Neutralizing antibody to human urinary CS factor neutralizes CS factor from other human and monkey sources. Higher concentrations of antiserum are required for neutralization of murine CS factor. Human urinary CS factor has similar properties to human and murine CS factors that stimulate the early appearance of macrophages in murine colonies. It is physically distinct from CS factors that stimulate the formation of pure granulocytic colonies by murine or human hemopoietic cells. Purified CS factor of the human urinary type is being used to investigate the nature of the interaction of CS factor with target cells and its physiological role in vivo."}
{"id": "PMID:1081457", "title": "[Responses of the units of the nerve of the frog semicircular canal to caloric stimuli of changing amplitude].", "content": "In curarized frogs, responses of isolated neural units of the lateral semicircular canal (LSC) to short local caloric stimuli of different amplitudes (0.5--12.0 degrees C over the canal temperature) inducing excitatory current of endolymph in the LSC, were studied. Estimation of maximal (peak) frequences (PF) in the responses showed the PFs in responses of 52% units (group A) to be either linearly or semilogarithmically related to peak temperature (PT). In 22% units (group B), PFs revealed saturation at PT8--12 degrees C. In 25% units (group C) PFs were related to PT first in direct and then--along with increase of PT--in reverse proportion. Duration of the group A responses is linearly for semilogarithmically related to PT. This was not observed in groups B and C. Two cases of correlation between spontaneous activity and dynamic characteristics of units from groups A and C were found. The results are in agreement with the literature references concerning data obtained in frogs using angular acceleration and stop-stimuli. The controlled caloric stimulus was concluded to be convenient for studying the dynamic characteristics of the semicircular canals afferents.", "contents": "[Responses of the units of the nerve of the frog semicircular canal to caloric stimuli of changing amplitude]. In curarized frogs, responses of isolated neural units of the lateral semicircular canal (LSC) to short local caloric stimuli of different amplitudes (0.5--12.0 degrees C over the canal temperature) inducing excitatory current of endolymph in the LSC, were studied. Estimation of maximal (peak) frequences (PF) in the responses showed the PFs in responses of 52% units (group A) to be either linearly or semilogarithmically related to peak temperature (PT). In 22% units (group B), PFs revealed saturation at PT8--12 degrees C. In 25% units (group C) PFs were related to PT first in direct and then--along with increase of PT--in reverse proportion. Duration of the group A responses is linearly for semilogarithmically related to PT. This was not observed in groups B and C. Two cases of correlation between spontaneous activity and dynamic characteristics of units from groups A and C were found. The results are in agreement with the literature references concerning data obtained in frogs using angular acceleration and stop-stimuli. The controlled caloric stimulus was concluded to be convenient for studying the dynamic characteristics of the semicircular canals afferents."}
{"id": "PMID:1081458", "title": "[The relationship between changes in the temporal and frequency characteristics of parasympathetic nerve activity and effects on the heart].", "content": "Changes of chronotropic effect on the frog heart on stimulation of part of extracardiac pathways, were studied. On the 10/sec stimulation during 1.5 sec, cardioinhibitory effect occurred. On 0.2 sec duration of the stimulation the positive chronotropic effect was observed. The heart rate increased on the 3/sec stimulation of the undegenerating extracardiac fibers by the 21st day after bilateral vagotomy; of the frequency of the stimulation was increased to 10/sec, the negative chronotropic effect occurred, and sympathectomy and the reserpine injection had no influence on the positive chrontropic effects. Both the negative and positive chronotropic effects could be prevented by atropine. The different chrontropic effects on the heart are, probably, due to activity of but a single functional type of fibers: the parasympathetic nervous fibers.", "contents": "[The relationship between changes in the temporal and frequency characteristics of parasympathetic nerve activity and effects on the heart]. Changes of chronotropic effect on the frog heart on stimulation of part of extracardiac pathways, were studied. On the 10/sec stimulation during 1.5 sec, cardioinhibitory effect occurred. On 0.2 sec duration of the stimulation the positive chronotropic effect was observed. The heart rate increased on the 3/sec stimulation of the undegenerating extracardiac fibers by the 21st day after bilateral vagotomy; of the frequency of the stimulation was increased to 10/sec, the negative chronotropic effect occurred, and sympathectomy and the reserpine injection had no influence on the positive chrontropic effects. Both the negative and positive chronotropic effects could be prevented by atropine. The different chrontropic effects on the heart are, probably, due to activity of but a single functional type of fibers: the parasympathetic nervous fibers."}
{"id": "PMID:1081459", "title": "Hemodynamic evaluation of patients with intrahepatic portal hypertension. Relationship between bleeding varices and the portohepatic gradient.", "content": "Between January 1970 and December 1974, 389 patients presenting an upper gastrointestinal hemorrhage had an emergency fiberoptic esophagogastroscopy at the time of bleeding. In 103 cases, esophageal and/or gastric varices were visualized and in 46 of these cases, bleeding originiated from varices. Hemodynamic data was available in 19 cases of intrahepatic portal hypertension who had bled from varices. Combined hepatic and umbilicoportal catherterization was perform in 17 cases whereas only hepatic vein catherterization was possible in 2. The portohepatic gradient (FPVP-FHVP or WHVP-FHVP) was utilized as an index of portal hypertension. In the 19 patients studied, the portohepatic gradient ranged from 12 to 24.5 mm Hg with a mean of 18.4 mm Hg; only one patient had a portohepatic gradient of less than 14.5 mm Hg. The present data strongly suggest that in intrahepatic portal hypertension, bleeding varices are observed only if significant portal hypertension exists. Hemodynamic studies may thus be helpful in evaluating bleeding patients with varices particularly when the origin of the bleeding cannot be determined at endoscopy. The finding of a portohepatic gradient of less than 12 mm Hg would strongly suggest that varices were not the source of bleeding and in these cases, shunt surgery should not be considered.", "contents": "Hemodynamic evaluation of patients with intrahepatic portal hypertension. Relationship between bleeding varices and the portohepatic gradient. Between January 1970 and December 1974, 389 patients presenting an upper gastrointestinal hemorrhage had an emergency fiberoptic esophagogastroscopy at the time of bleeding. In 103 cases, esophageal and/or gastric varices were visualized and in 46 of these cases, bleeding originiated from varices. Hemodynamic data was available in 19 cases of intrahepatic portal hypertension who had bled from varices. Combined hepatic and umbilicoportal catherterization was perform in 17 cases whereas only hepatic vein catherterization was possible in 2. The portohepatic gradient (FPVP-FHVP or WHVP-FHVP) was utilized as an index of portal hypertension. In the 19 patients studied, the portohepatic gradient ranged from 12 to 24.5 mm Hg with a mean of 18.4 mm Hg; only one patient had a portohepatic gradient of less than 14.5 mm Hg. The present data strongly suggest that in intrahepatic portal hypertension, bleeding varices are observed only if significant portal hypertension exists. Hemodynamic studies may thus be helpful in evaluating bleeding patients with varices particularly when the origin of the bleeding cannot be determined at endoscopy. The finding of a portohepatic gradient of less than 12 mm Hg would strongly suggest that varices were not the source of bleeding and in these cases, shunt surgery should not be considered."}
{"id": "PMID:1081463", "title": "Peripheral blood and intestinal lymphocyte sub-populations in Crohn's disease.", "content": "Immunofluorescent technques were utilized to study the nature and distribution of cells forming the inflammatory infiltrate in Crohn's disease. Tissue from resected ileum (one patient) or colon (one patient) when compared with corresponding control tissues contained increased numbers of B and T lymphocytes. B-cells of IgG class predominated in the mucosal lamina propria, whereas T-cells were prominent in the deeper layers of inflamed bowel. Inflammatory cells bearing Fc receptors were also present in these tissues. Decreased peripheral blood T-cell numbers returned to normal after resection of the involved bowel in the two patients studied. Impaired cellular immunity in Crohn's disease may thus result from T-cell sequestration in or loss from involved segments of intestine.", "contents": "Peripheral blood and intestinal lymphocyte sub-populations in Crohn's disease. Immunofluorescent technques were utilized to study the nature and distribution of cells forming the inflammatory infiltrate in Crohn's disease. Tissue from resected ileum (one patient) or colon (one patient) when compared with corresponding control tissues contained increased numbers of B and T lymphocytes. B-cells of IgG class predominated in the mucosal lamina propria, whereas T-cells were prominent in the deeper layers of inflamed bowel. Inflammatory cells bearing Fc receptors were also present in these tissues. Decreased peripheral blood T-cell numbers returned to normal after resection of the involved bowel in the two patients studied. Impaired cellular immunity in Crohn's disease may thus result from T-cell sequestration in or loss from involved segments of intestine."}
{"id": "PMID:1081464", "title": "A prospective comparative trial between early endoscopy and radiology in acute upper gastrointestinal haemorrhage.", "content": "A prospective comparative trial is reported between the results of early endoscopy and barium meal examinations in 150 patients admitted with acute upper gastrointestinal haemorrhage. One hundred and thirty-eight patients underwent both investigations within 24 hours of admission. Emergency endoscopy accurately identified the source of haemorrhage in 86% of patients and emergency radiology identified the source in 51%. Misleading positive reports were given endoscopically in 3% and by barium meal in 8%. The identification of the source of haemorrhage was possible in 126 patients (91%) by the use of both methods. Emergency endoscopy is shown to be superior to emergency barium meal examination, but the examinations are complementary and both should be used for these patients.", "contents": "A prospective comparative trial between early endoscopy and radiology in acute upper gastrointestinal haemorrhage. A prospective comparative trial is reported between the results of early endoscopy and barium meal examinations in 150 patients admitted with acute upper gastrointestinal haemorrhage. One hundred and thirty-eight patients underwent both investigations within 24 hours of admission. Emergency endoscopy accurately identified the source of haemorrhage in 86% of patients and emergency radiology identified the source in 51%. Misleading positive reports were given endoscopically in 3% and by barium meal in 8%. The identification of the source of haemorrhage was possible in 126 patients (91%) by the use of both methods. Emergency endoscopy is shown to be superior to emergency barium meal examination, but the examinations are complementary and both should be used for these patients."}
{"id": "PMID:1081483", "title": "Does alpha-1-antitrypsin P1 null phenotype exist?", "content": "A second case of Pi null alpha-1-antitrypsin (AA) deficiency is described. In fact, the serum's subject contains 5 mug of AA per millilitre. With radiolabelled specific antibodies, it is possible to describe the Pi phenotype associated to this deficiency. The pattern which is obtained is like the ordinary Pi M, but 500 times lower than normal values. In contrast to a common deficient variant (ZZ or MZ), the subject tissues do not contain periodic acid-schiff positive inclusion bodies. The \"normal\" pattern obtained after antigen-antibody crossed electrophoresis, would be in favour of a deficient anomaly hereditarily transmitted.", "contents": "Does alpha-1-antitrypsin P1 null phenotype exist? A second case of Pi null alpha-1-antitrypsin (AA) deficiency is described. In fact, the serum's subject contains 5 mug of AA per millilitre. With radiolabelled specific antibodies, it is possible to describe the Pi phenotype associated to this deficiency. The pattern which is obtained is like the ordinary Pi M, but 500 times lower than normal values. In contrast to a common deficient variant (ZZ or MZ), the subject tissues do not contain periodic acid-schiff positive inclusion bodies. The \"normal\" pattern obtained after antigen-antibody crossed electrophoresis, would be in favour of a deficient anomaly hereditarily transmitted."}
{"id": "PMID:1081484", "title": "Chromosomal banding of cultured T and B lymphocytes.", "content": "Chromosomes of thymus-derived lymphocyte (T-cell) cell lines (CCRF-CEM, CCRF-H-SB-2, MOLT-3, MOLT-4, and RPMI 8402) and the corresponding lines (CCRF-SB and RPMI 8392) with thymus-independent lymphocyte (B-cell) characteristics, were examined with banding techniques. The pairs of cell lines (CCRF-H-SB-2 and CCRF-SB, and RPMI 8402 and RPMI 8392, were confirmed to be of the same origin, respectively, inasmuch as their individual specific variant bands were identical. The MOLT-3 and MOLT-4 cells had common marker chromosomes, indicating that the chromosomal changes in these cells probably occurred in vivo. All of the T-cells had structurally abnormal marker chromosomes, whereas the B-cells were chromsomally normal. The structural changes of the chromosomes suggest that the malignant nature of the present T-cells may be essential for such T-cells to be established in vitro in a standard culture medium.", "contents": "Chromosomal banding of cultured T and B lymphocytes. Chromosomes of thymus-derived lymphocyte (T-cell) cell lines (CCRF-CEM, CCRF-H-SB-2, MOLT-3, MOLT-4, and RPMI 8402) and the corresponding lines (CCRF-SB and RPMI 8392) with thymus-independent lymphocyte (B-cell) characteristics, were examined with banding techniques. The pairs of cell lines (CCRF-H-SB-2 and CCRF-SB, and RPMI 8402 and RPMI 8392, were confirmed to be of the same origin, respectively, inasmuch as their individual specific variant bands were identical. The MOLT-3 and MOLT-4 cells had common marker chromosomes, indicating that the chromosomal changes in these cells probably occurred in vivo. All of the T-cells had structurally abnormal marker chromosomes, whereas the B-cells were chromsomally normal. The structural changes of the chromosomes suggest that the malignant nature of the present T-cells may be essential for such T-cells to be established in vitro in a standard culture medium."}
{"id": "PMID:1081487", "title": "Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix.", "content": "The specificity and cross-species activity of thymic extracts and some non-specific agents on some alleged properties of T cells and their precursors in the bone marrow cells of normal, nude and thymectomized mice was studied in vitro. The phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) was used to assess the maturation and responses of cells treated with the extracts to mitogens, i.e. phytohaemagglutinin and concanavalin A. We found that thymic extracts of calf and mouse origin induce functional 'maturation' which can be abrogated by cycloheximide, an inhibitor of protein synthesis. The nonspecific agents used (spleen extracts, PPD, dBcAMP and endotoxin) had no effect on bone marrow cells, as measured by changes in the SCM. The method of measuring SCM using the technique of fluorescence polarization is described.", "contents": "Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix. The specificity and cross-species activity of thymic extracts and some non-specific agents on some alleged properties of T cells and their precursors in the bone marrow cells of normal, nude and thymectomized mice was studied in vitro. The phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) was used to assess the maturation and responses of cells treated with the extracts to mitogens, i.e. phytohaemagglutinin and concanavalin A. We found that thymic extracts of calf and mouse origin induce functional 'maturation' which can be abrogated by cycloheximide, an inhibitor of protein synthesis. The nonspecific agents used (spleen extracts, PPD, dBcAMP and endotoxin) had no effect on bone marrow cells, as measured by changes in the SCM. The method of measuring SCM using the technique of fluorescence polarization is described."}
{"id": "PMID:1081488", "title": "Distribution of electrophoretic mobilities of mouse thymocyte subpopulations in the presence of tumour cells.", "content": "Analysis of the electrophoretic mobility of mouse thymus cells has showed two main populations, with mean mobility values of 0-77 +/- 0-023 mum s-1 V-1cm and 0-99 +/- 0-015 mum s-1P1cm; these absolute values varied slightly from one strain to another. Implantation of tumour cells caused the relative proportions of these two populations to change dramatically within 48 hours, when an increase in the fast-moving 'immunocompetent' thymocytes was observed. The ratio of slow to fast cells changed from 9:1 in the normal BALB/c animal to 2:1 in the presence of the tumour cells and this 2:1 ratio persisted throughout the remainder of the animal's life. However, inoculation of histocompatible spleen cells from a normal individual evoked only a brief response in the host's thymus. This change in ratio of slow to fast cells in thethymus was interpreted as an increased production of immunocompetent cells in response to the presence of the tumour cells.", "contents": "Distribution of electrophoretic mobilities of mouse thymocyte subpopulations in the presence of tumour cells. Analysis of the electrophoretic mobility of mouse thymus cells has showed two main populations, with mean mobility values of 0-77 +/- 0-023 mum s-1 V-1cm and 0-99 +/- 0-015 mum s-1P1cm; these absolute values varied slightly from one strain to another. Implantation of tumour cells caused the relative proportions of these two populations to change dramatically within 48 hours, when an increase in the fast-moving 'immunocompetent' thymocytes was observed. The ratio of slow to fast cells changed from 9:1 in the normal BALB/c animal to 2:1 in the presence of the tumour cells and this 2:1 ratio persisted throughout the remainder of the animal's life. However, inoculation of histocompatible spleen cells from a normal individual evoked only a brief response in the host's thymus. This change in ratio of slow to fast cells in thethymus was interpreted as an increased production of immunocompetent cells in response to the presence of the tumour cells."}
{"id": "PMID:1081489", "title": "RRBC and EAC rosette formation after DNCB contact sensitization and cyclophosphamide treatment of guinea-pigs.", "content": "Rabbit erythrocyte (RRBC) and EAC rosette formation, for detection of T and B lymphocytes respectively, was studied in thymus, lymph nodes and peripheral blood of guinea-pigs. Using two different methods for preparation of RRBC rosette-forming cells (RFC) it was found that, without an incubation period at 37 degrees, a smaller part of the pool of T cells is detected which is particularly sensitive to cyclophosphamide (cy). RRBC rosette formation was studied for 2 weeks after treatment with one high dose of cy and appeared to be minimal in the thymus after 1 week and maximal in the lymph nodes at about day 3. EAC rosette formation was completely blocked by cy treatment. Four days after contact sensitization with 2,4-dinitrochlorobenzene (DNCB) no percentual increase was found in RRBC-RFC, suggesting a parallel increase of B and T cells in stimulated lymph nodes. Cytological screening of the rosettes at day 4 after DNCB showed that of the RRBC-RFC approximately 17 per cent were blast cells, while no difference was detected in the number of blast cells participating in EAC rosette formation as compared to the unstimulated lymph nodes. In contrast to RRBC-RFC the percentage of EAC-RFC in draining lymph nodes is increased after contact sensitization. The mechanism of this increase is discussed.", "contents": "RRBC and EAC rosette formation after DNCB contact sensitization and cyclophosphamide treatment of guinea-pigs. Rabbit erythrocyte (RRBC) and EAC rosette formation, for detection of T and B lymphocytes respectively, was studied in thymus, lymph nodes and peripheral blood of guinea-pigs. Using two different methods for preparation of RRBC rosette-forming cells (RFC) it was found that, without an incubation period at 37 degrees, a smaller part of the pool of T cells is detected which is particularly sensitive to cyclophosphamide (cy). RRBC rosette formation was studied for 2 weeks after treatment with one high dose of cy and appeared to be minimal in the thymus after 1 week and maximal in the lymph nodes at about day 3. EAC rosette formation was completely blocked by cy treatment. Four days after contact sensitization with 2,4-dinitrochlorobenzene (DNCB) no percentual increase was found in RRBC-RFC, suggesting a parallel increase of B and T cells in stimulated lymph nodes. Cytological screening of the rosettes at day 4 after DNCB showed that of the RRBC-RFC approximately 17 per cent were blast cells, while no difference was detected in the number of blast cells participating in EAC rosette formation as compared to the unstimulated lymph nodes. In contrast to RRBC-RFC the percentage of EAC-RFC in draining lymph nodes is increased after contact sensitization. The mechanism of this increase is discussed."}
{"id": "PMID:1081490", "title": "Antigen-initiated B-lymphocyte differentiation. VII. Quantification of AFC progenitor levels in adoptive and culture responses to NIP-POL antigen.", "content": "Quantitative studies on B cells require a direct assay for antibody-forming cell (AFC) progenitor function, in which the number of AFC produced bears a simple, linear arithmetic relationship to the number of progenitors present. This might be expected under conditions where helper T-cell and accessory cell requirements are by-passed, or provided in excess. This possibility has been tested using as antigen the hapten NIP (4-hydroxy-3-iodo-5-nitrophenylacetic acid) on the carrier POL (polymerized bacterial flagellin), in adoptive transfer of normal and nude mouse spleen cells to irradiated recipients, and in cell culture. Primary and secondary IgM responses to this antigen are \"T cell-independent'. The secondard IgG response is T cell-dependent but this function can be provided by 'carrier-primed' irradiated recipients. However in no case did the cell dose response curve show a linear, arithmetic relationship between cells transferred or cultured, and AFC produced. If less than 10 X 10(6) cells were adoptively transferred or cultured, a sigmoid curve was obtained, approximately linear with a slope of around 1-6 on a log-log scale. In adoptive transfer, a plateau was then seen above 10 X 10(6) cells, followed by a second sharp rise beginning around 15 X 10(6) cells. Addition of irradiated spleen cells as 'fillers' to maintain cell numbers constant produced a linear (arithmetic scale) dose response curve for the primary IgM responses, both adoptive and in culture. Lipopolysaccharide injection of recipients also produced linear regions in the adoptive transfer system. These techniques provide more direct, quantitative assay systems for the primary IgM responses to this antigen. However, arithmetic linear cell dose response curves were still not obtained for the secondary IgG responses, using irradiated filler cells.", "contents": "Antigen-initiated B-lymphocyte differentiation. VII. Quantification of AFC progenitor levels in adoptive and culture responses to NIP-POL antigen. Quantitative studies on B cells require a direct assay for antibody-forming cell (AFC) progenitor function, in which the number of AFC produced bears a simple, linear arithmetic relationship to the number of progenitors present. This might be expected under conditions where helper T-cell and accessory cell requirements are by-passed, or provided in excess. This possibility has been tested using as antigen the hapten NIP (4-hydroxy-3-iodo-5-nitrophenylacetic acid) on the carrier POL (polymerized bacterial flagellin), in adoptive transfer of normal and nude mouse spleen cells to irradiated recipients, and in cell culture. Primary and secondary IgM responses to this antigen are \"T cell-independent'. The secondard IgG response is T cell-dependent but this function can be provided by 'carrier-primed' irradiated recipients. However in no case did the cell dose response curve show a linear, arithmetic relationship between cells transferred or cultured, and AFC produced. If less than 10 X 10(6) cells were adoptively transferred or cultured, a sigmoid curve was obtained, approximately linear with a slope of around 1-6 on a log-log scale. In adoptive transfer, a plateau was then seen above 10 X 10(6) cells, followed by a second sharp rise beginning around 15 X 10(6) cells. Addition of irradiated spleen cells as 'fillers' to maintain cell numbers constant produced a linear (arithmetic scale) dose response curve for the primary IgM responses, both adoptive and in culture. Lipopolysaccharide injection of recipients also produced linear regions in the adoptive transfer system. These techniques provide more direct, quantitative assay systems for the primary IgM responses to this antigen. However, arithmetic linear cell dose response curves were still not obtained for the secondary IgG responses, using irradiated filler cells."}
{"id": "PMID:1081491", "title": "Clonal proliferation of PHA-stimulated human lymphocytes in soft agar culture.", "content": "The purpose of this investigation was the induction of clonal proliferation of PHA-stimulated normal human lymphocytes using a two-layer soft agar technique. Essential conditions for colony formation include preceding sensitization of lymphocytes with PHA, and continuous presence of PHA in the soft agar culture. Two types of colonies developed: large colonies which appeared 3-4 days after seeding and comprised, after 5-6 days, 200-500 cells, and small colonies which were seen after 6-7 days of culture, resulting in production of 50-150 cells. Morphological study showed that all cells were blast-like and the mitotic index exceeded that in liquid medium by a factor of 50. Comparison between the number of colonies developing from cultured bone marrow and spleen cells with those from peripheral blood showed that, in proportion to the number of lymphocytes seeded, a larger number of colonies developed from bone marrow cells and a lower number of colonies developed from spleen  cells. The time required for sensitization of lymphocytes in liquid medium with PHA was found to be no less than 12 hours. The greatest number of colonies appeared when the optimal concentration of PHA was placed in the lower agar layer. A linear relation between the number of cells seeded and the number of resulting colonies was found. One out of 2 X 10(3) or 3 X 10(3) lymphocytes in peripheral blood has the potential to develop as colony. The rosette-forming ability and morphological identification of the cells suggest that the colonies are composed of T lymphocytes.", "contents": "Clonal proliferation of PHA-stimulated human lymphocytes in soft agar culture. The purpose of this investigation was the induction of clonal proliferation of PHA-stimulated normal human lymphocytes using a two-layer soft agar technique. Essential conditions for colony formation include preceding sensitization of lymphocytes with PHA, and continuous presence of PHA in the soft agar culture. Two types of colonies developed: large colonies which appeared 3-4 days after seeding and comprised, after 5-6 days, 200-500 cells, and small colonies which were seen after 6-7 days of culture, resulting in production of 50-150 cells. Morphological study showed that all cells were blast-like and the mitotic index exceeded that in liquid medium by a factor of 50. Comparison between the number of colonies developing from cultured bone marrow and spleen cells with those from peripheral blood showed that, in proportion to the number of lymphocytes seeded, a larger number of colonies developed from bone marrow cells and a lower number of colonies developed from spleen  cells. The time required for sensitization of lymphocytes in liquid medium with PHA was found to be no less than 12 hours. The greatest number of colonies appeared when the optimal concentration of PHA was placed in the lower agar layer. A linear relation between the number of cells seeded and the number of resulting colonies was found. One out of 2 X 10(3) or 3 X 10(3) lymphocytes in peripheral blood has the potential to develop as colony. The rosette-forming ability and morphological identification of the cells suggest that the colonies are composed of T lymphocytes."}
{"id": "PMID:1081492", "title": "Local immune response in experimental pyelonephritis in the rabbit. I. Morphological and functional features of the lymphocytic infiltrate.", "content": "The cellular activity of circulating lymphocytes and lymphocytes isolated from the infected kidney of animals with experimental haematogenous pyelonephritis was evaluated. The incorporation of [3H-methyl]thymidine into DNA by lymphocytes was studied with mitogens such as phytohaemagglutinin (PHA), pokeweek mitogen (PWM) and goat anti-rabbit IgG (GARIG). Lymphocytes from infected kidney had a high baseline DNA synthesis compared to circulating lymphocytes from days 5 to 27 of infection. Infected kidney lymphocytes failed to respond to PHA, PWM, or GARIG, whereas circulating lymphocytes did respond to these mitogens. Uropod-bearing lymphocytes, which were shown to be T lymphocytes, were present from days 5 to 77 of infection. B lymphocytes, as determined by surface immunofluorescent technique, were present by day 12, coincident with the onset of local synthesis of antibody. These studies reveal that in pyelonephritis, the cellular response goes through sequential changes and indicate a dynamic interrelationship between T and B lymphocytes at an infected site.", "contents": "Local immune response in experimental pyelonephritis in the rabbit. I. Morphological and functional features of the lymphocytic infiltrate. The cellular activity of circulating lymphocytes and lymphocytes isolated from the infected kidney of animals with experimental haematogenous pyelonephritis was evaluated. The incorporation of [3H-methyl]thymidine into DNA by lymphocytes was studied with mitogens such as phytohaemagglutinin (PHA), pokeweek mitogen (PWM) and goat anti-rabbit IgG (GARIG). Lymphocytes from infected kidney had a high baseline DNA synthesis compared to circulating lymphocytes from days 5 to 27 of infection. Infected kidney lymphocytes failed to respond to PHA, PWM, or GARIG, whereas circulating lymphocytes did respond to these mitogens. Uropod-bearing lymphocytes, which were shown to be T lymphocytes, were present from days 5 to 77 of infection. B lymphocytes, as determined by surface immunofluorescent technique, were present by day 12, coincident with the onset of local synthesis of antibody. These studies reveal that in pyelonephritis, the cellular response goes through sequential changes and indicate a dynamic interrelationship between T and B lymphocytes at an infected site."}
{"id": "PMID:1081493", "title": "Relationship between biological activities of polymers. I. Immunogenicity, C3 activation, mitogenicity for B cells and adjuvant properties.", "content": "The effect of thirteen compounds on C3 activation, mitogenicity for B lymphocytes and on the immune response was tested. Only two of the compounds tested, polyinosinic acid and a high molecular weight polyethylenesulphate induced C3 conversion, while eleven out of the thirteen compounds (negative charged immunogenic or non-immunogenic polymers including the three C3 active compounds) stimulated thymidine incorporation in B lymphocytes. The non-charged (T-independent) immunogens dextran and polyvinylpyrrolidone neither activated C3 nor BP lymphocytes. With the exception of single-stranded polyribonucleic acids, the compounds stimulating DNA synthetis in B cells enhanced the response of the mice to a suboptimal dose of sheep red blood cells. In conclusion C3 activation, intrinsic (T-independent) immunogenicity and B-cell stimulation are not correlated properties. However, B-cell mitogenicity and adjuvanticity seem to be related.", "contents": "Relationship between biological activities of polymers. I. Immunogenicity, C3 activation, mitogenicity for B cells and adjuvant properties. The effect of thirteen compounds on C3 activation, mitogenicity for B lymphocytes and on the immune response was tested. Only two of the compounds tested, polyinosinic acid and a high molecular weight polyethylenesulphate induced C3 conversion, while eleven out of the thirteen compounds (negative charged immunogenic or non-immunogenic polymers including the three C3 active compounds) stimulated thymidine incorporation in B lymphocytes. The non-charged (T-independent) immunogens dextran and polyvinylpyrrolidone neither activated C3 nor BP lymphocytes. With the exception of single-stranded polyribonucleic acids, the compounds stimulating DNA synthetis in B cells enhanced the response of the mice to a suboptimal dose of sheep red blood cells. In conclusion C3 activation, intrinsic (T-independent) immunogenicity and B-cell stimulation are not correlated properties. However, B-cell mitogenicity and adjuvanticity seem to be related."}
{"id": "PMID:1081494", "title": "Cell-mediated allograft responses in vitro. VI. Studies on macrophage-mediated cytotoxicity.", "content": "Normal murine peritoneal macrophages were rendered cytotoxic against 51Cr-labelled allogeneic and syngeneic target cells by incubation with supernatant of selected cell cultures.'Active' culture supernatant was produced both by specifically sensitized cytotoxic T lymphocytes as well as by mitogen-stimulated T cells, but not by mitogen-stimulated B cells. The in vitro induced macrophage-mediated cytotoxicity was found to be non-specific in the sense that 51Cr-labelled target cells of different H-2 haplotype were lysed equally well.", "contents": "Cell-mediated allograft responses in vitro. VI. Studies on macrophage-mediated cytotoxicity. Normal murine peritoneal macrophages were rendered cytotoxic against 51Cr-labelled allogeneic and syngeneic target cells by incubation with supernatant of selected cell cultures.'Active' culture supernatant was produced both by specifically sensitized cytotoxic T lymphocytes as well as by mitogen-stimulated T cells, but not by mitogen-stimulated B cells. The in vitro induced macrophage-mediated cytotoxicity was found to be non-specific in the sense that 51Cr-labelled target cells of different H-2 haplotype were lysed equally well."}
{"id": "PMID:1081495", "title": "A proposition on the distribution of antibody affinities, with implications for the mechanism of B-cell activation.", "content": "For most antisera a linear relationship can be shown between log antigen concentration and the log concentration of antibody required to bind half the available antigen. This paper shows that the slope of this line, s, is related to the distribution of individual antibody clonotypes of different affinity. It is argued that the general form of the distribution approximates to exponential (rather than for example Gaussian) and that this indicates a requirement for some force to be exerted through the antigen-receptor bond in order to activate a B cell. An alternative parameter, A, which gives more weight to high affinity clonotypes, is offered in place of K0 as a measure of the avidity and biological effectiveness of an antiserum.", "contents": "A proposition on the distribution of antibody affinities, with implications for the mechanism of B-cell activation. For most antisera a linear relationship can be shown between log antigen concentration and the log concentration of antibody required to bind half the available antigen. This paper shows that the slope of this line, s, is related to the distribution of individual antibody clonotypes of different affinity. It is argued that the general form of the distribution approximates to exponential (rather than for example Gaussian) and that this indicates a requirement for some force to be exerted through the antigen-receptor bond in order to activate a B cell. An alternative parameter, A, which gives more weight to high affinity clonotypes, is offered in place of K0 as a measure of the avidity and biological effectiveness of an antiserum."}
{"id": "PMID:1081496", "title": "Quantitation of potential T-lymphocyte function in rats.", "content": "A simple but dependable method for determining the functional capacity of thymus-derived lymphocytes is described. The in vitro culture conditions for determining the incorporation of tritiated thymidine after stimulation with phytohemagglutinin and other mitogens have been given in detail and the methodology confirmed by measuring the ablation of T-cell function in T cell depleted animals.", "contents": "Quantitation of potential T-lymphocyte function in rats. A simple but dependable method for determining the functional capacity of thymus-derived lymphocytes is described. The in vitro culture conditions for determining the incorporation of tritiated thymidine after stimulation with phytohemagglutinin and other mitogens have been given in detail and the methodology confirmed by measuring the ablation of T-cell function in T cell depleted animals."}
{"id": "PMID:1081497", "title": "Increase in the proportion of cells with the C'3 receptor in BALB/c mice bearing mammary tumors.", "content": "Transplantation of mammary tumors originally induced by dimethylbenzanthracene caused splenomegaly and an increase in the total population of spleen cells paralleling the increase in tumor size. At least part of the hypercellularity was due to in situ proliferation as evidenced by the increased number of blast forms and mitotic figures. The cells were characterized with respect to parameters generally associated with B-cells; surface immunoglobulins as detected by immunofluorescence and C'3 receptors as determined by rosette formation with sheep red blood cells (SRBC) coated with rabbit anti-SRBC and mouse complement. There was no change in the percentage of B-cells with surface immunoglobulin in tumor-bearing mice compared with the control animals. However, there was a profound change in the representation of cells bearing C'3 receptors. The percentage of the cells increased dramatically with tumor growth. Various possibilities regarding the nature and function of these cells are considered.", "contents": "Increase in the proportion of cells with the C'3 receptor in BALB/c mice bearing mammary tumors. Transplantation of mammary tumors originally induced by dimethylbenzanthracene caused splenomegaly and an increase in the total population of spleen cells paralleling the increase in tumor size. At least part of the hypercellularity was due to in situ proliferation as evidenced by the increased number of blast forms and mitotic figures. The cells were characterized with respect to parameters generally associated with B-cells; surface immunoglobulins as detected by immunofluorescence and C'3 receptors as determined by rosette formation with sheep red blood cells (SRBC) coated with rabbit anti-SRBC and mouse complement. There was no change in the percentage of B-cells with surface immunoglobulin in tumor-bearing mice compared with the control animals. However, there was a profound change in the representation of cells bearing C'3 receptors. The percentage of the cells increased dramatically with tumor growth. Various possibilities regarding the nature and function of these cells are considered."}
{"id": "PMID:1081500", "title": "Evaluation of radiochemical protectors using urinary deoxycytidine levels.", "content": "It is customary to use the LD50 response as a criterion for the evaluation of chemical radioprotection. The increased urinary excretion of deoxycytidine (CdR) in irradiated rats has been shown to be a linear dose--response relationship up to 200 R, thus making it possible to consider such a response as a criterion for chemical radioprotection evaluation at sub-lethal irradiation levels. Rats were subjected to 200 R X-irradiation with various chemical protectors and without chemical protectors, urine was collected 24 hours after irradiation, and the CdR levels in the urine analysed. A dose-reduction factor (DRF) was determined for 24-hour urinary excretions of CdR for chemically protected and non-protected rats. The DRF values are within 6 per cent of those reported by other investigators using LD50 as the response criterion for serotonin, cysteine, MEA and PAPP but differ for WR-2721 and WR-2823. Agreement using WR-2721 and WR-2823 compounds is only obtained when the toxicity effect of the compound is taken into consideration.", "contents": "Evaluation of radiochemical protectors using urinary deoxycytidine levels. It is customary to use the LD50 response as a criterion for the evaluation of chemical radioprotection. The increased urinary excretion of deoxycytidine (CdR) in irradiated rats has been shown to be a linear dose--response relationship up to 200 R, thus making it possible to consider such a response as a criterion for chemical radioprotection evaluation at sub-lethal irradiation levels. Rats were subjected to 200 R X-irradiation with various chemical protectors and without chemical protectors, urine was collected 24 hours after irradiation, and the CdR levels in the urine analysed. A dose-reduction factor (DRF) was determined for 24-hour urinary excretions of CdR for chemically protected and non-protected rats. The DRF values are within 6 per cent of those reported by other investigators using LD50 as the response criterion for serotonin, cysteine, MEA and PAPP but differ for WR-2721 and WR-2823. Agreement using WR-2721 and WR-2823 compounds is only obtained when the toxicity effect of the compound is taken into consideration."}
{"id": "PMID:1081501", "title": "Effects of DTPA on the deposition of 65Zn, 60Co and 144Ce in pregnant rat and in foetoplacental unit.", "content": "65Zn, 60Co or 144Ce was injected intravenously into rats at various stages of pregnancy. Six hours later, DTPA chelate of non-radioactive Ca, Zn or Co was given intraperitoneally. Another group of pregnant rats received intravenous injection of 65ZnDTPA or 60CoDTPA. In both experiments, 24 hours after administration, the concentration of the radioactive material was determined in the dam's whole body as well as in foetuses, chorioallantoic placentae and yolk sacs. 65Zn and 60Co, which had been administered to the rats as DTPA complexes, were found not to pass from the dam's blood to the foetuses or to pass only in very minute amounts. This finding could be considered as an indirect indication that DTPA chelates do not penetrate the placental barrier. Nevertheless, the chelates injected intravenously into the dam early after an internal contamination, decrease the radionuclide content in the foetuses. No substantial effects of pregnancy on the effectiveness of the chelate in removing radionuclides from the body of rats were observed.", "contents": "Effects of DTPA on the deposition of 65Zn, 60Co and 144Ce in pregnant rat and in foetoplacental unit. 65Zn, 60Co or 144Ce was injected intravenously into rats at various stages of pregnancy. Six hours later, DTPA chelate of non-radioactive Ca, Zn or Co was given intraperitoneally. Another group of pregnant rats received intravenous injection of 65ZnDTPA or 60CoDTPA. In both experiments, 24 hours after administration, the concentration of the radioactive material was determined in the dam's whole body as well as in foetuses, chorioallantoic placentae and yolk sacs. 65Zn and 60Co, which had been administered to the rats as DTPA complexes, were found not to pass from the dam's blood to the foetuses or to pass only in very minute amounts. This finding could be considered as an indirect indication that DTPA chelates do not penetrate the placental barrier. Nevertheless, the chelates injected intravenously into the dam early after an internal contamination, decrease the radionuclide content in the foetuses. No substantial effects of pregnancy on the effectiveness of the chelate in removing radionuclides from the body of rats were observed."}
{"id": "PMID:1081498", "title": "Lumphocytotoxins in leprosy.", "content": "Occurrence of cold lymphocytotoxins has been observed in 59 leprosy sera. In 46% of the patients, cold lymphocytotoxins were present whereas in helathy controls only 13% showed such antibodies. The highest incidence of alloantibodies was detected in lepromatous leprosy. Levels of autoantibodies, immunoglobulins and C3 were tested in parallel without finding any significnat correlations.", "contents": "Lumphocytotoxins in leprosy. Occurrence of cold lymphocytotoxins has been observed in 59 leprosy sera. In 46% of the patients, cold lymphocytotoxins were present whereas in helathy controls only 13% showed such antibodies. The highest incidence of alloantibodies was detected in lepromatous leprosy. Levels of autoantibodies, immunoglobulins and C3 were tested in parallel without finding any significnat correlations."}
{"id": "PMID:1081502", "title": "Radiation-induced decrease in influx rates of potassium ions into thymocytes in vitro in relation to decreased intracellular adenosine triphosphate concentrations.", "content": "The relationship between decreases in the adenosine triphosphate content of irradiated thymocytes and observed changes in the influx constant values for potassium ions in the same cells have been investigated. The results suggest that radiation-reduced ATP concentrations may be a rate-limiting factor controlling the active transport of potassium, 3--4 hours after X-irradiation in the range 2--20 krad. However, over the first hour of incubation, post-exposure ATP levels have not decreased sufficiently to be a major factor in the observed decrease in active uptake of potassium ions. This evidence is supported by comparisons of the influence of 1 mM adenine on radiation-reduced ATP levels and influx constants over the first hour after irradiation.", "contents": "Radiation-induced decrease in influx rates of potassium ions into thymocytes in vitro in relation to decreased intracellular adenosine triphosphate concentrations. The relationship between decreases in the adenosine triphosphate content of irradiated thymocytes and observed changes in the influx constant values for potassium ions in the same cells have been investigated. The results suggest that radiation-reduced ATP concentrations may be a rate-limiting factor controlling the active transport of potassium, 3--4 hours after X-irradiation in the range 2--20 krad. However, over the first hour of incubation, post-exposure ATP levels have not decreased sufficiently to be a major factor in the observed decrease in active uptake of potassium ions. This evidence is supported by comparisons of the influence of 1 mM adenine on radiation-reduced ATP levels and influx constants over the first hour after irradiation."}
{"id": "PMID:1081499", "title": "Leprosy XII. Quantitative analysis of thymus-derived lymphocyte response to phytohemagglutinin in leprosy.", "content": "The immune status of various leprosy patients was evaluated by using a micromethod to evaluate lymphocyte responses to phytohemagglutinin (PHA). In our study, whole blood was used and the degree of response to PHA stimulation was expressed in terms of unit volume of blood. A markedly decreased response to PHA stimulation was noted in patients with active lepromatous leprosy. Patients with active lepromatous  leprosy who have been proved drug (DDS) resistant showed less response than did those of drug sensitive patients with active lepromatous disease, while the patients with active lepromatous leprosy complicated by erythema nodosum leprosum (ENL) showed higher response than did those of patients with no complicated ENL. Comparing the results obtained to those obtained using other methods for T cell analysis indicates that these results reflect the number of T lymphocytes in the leprosy patient. Thus, this simple method is of value in assaying the presence and responses of T lymphocytes in the leprosy patient.", "contents": "Leprosy XII. Quantitative analysis of thymus-derived lymphocyte response to phytohemagglutinin in leprosy. The immune status of various leprosy patients was evaluated by using a micromethod to evaluate lymphocyte responses to phytohemagglutinin (PHA). In our study, whole blood was used and the degree of response to PHA stimulation was expressed in terms of unit volume of blood. A markedly decreased response to PHA stimulation was noted in patients with active lepromatous leprosy. Patients with active lepromatous  leprosy who have been proved drug (DDS) resistant showed less response than did those of drug sensitive patients with active lepromatous disease, while the patients with active lepromatous leprosy complicated by erythema nodosum leprosum (ENL) showed higher response than did those of patients with no complicated ENL. Comparing the results obtained to those obtained using other methods for T cell analysis indicates that these results reflect the number of T lymphocytes in the leprosy patient. Thus, this simple method is of value in assaying the presence and responses of T lymphocytes in the leprosy patient."}
{"id": "PMID:1081509", "title": "Alterations of liver DNA after x-irradiation. Reassociation and hybridization with nuclear RNA.", "content": "When RNA is extracted either from irradiated or unirradiated rat livers, no difference in its ability to hybridize with DNA is detectable. In contrast, the ability of DNA extracted from irradiated animals to hybirdize with RNA is decreased, probably as a result of DNA fragmentation. Sedimentation of X-irradiated DNA on sucrose gradients separates a small (low molecular weight) from a large peak (high molecular weight). The hybridization capacity for RNA of the large molecular weight DNA is similar to that of unirradiated DNA, but that of the small molecular weight is DNA reduced. After irradiation of the denatured DNA in vitro, the reassociation of DNA is inhibited, which suggests that DNA strands have lost part of their complementariness.", "contents": "Alterations of liver DNA after x-irradiation. Reassociation and hybridization with nuclear RNA. When RNA is extracted either from irradiated or unirradiated rat livers, no difference in its ability to hybridize with DNA is detectable. In contrast, the ability of DNA extracted from irradiated animals to hybirdize with RNA is decreased, probably as a result of DNA fragmentation. Sedimentation of X-irradiated DNA on sucrose gradients separates a small (low molecular weight) from a large peak (high molecular weight). The hybridization capacity for RNA of the large molecular weight DNA is similar to that of unirradiated DNA, but that of the small molecular weight is DNA reduced. After irradiation of the denatured DNA in vitro, the reassociation of DNA is inhibited, which suggests that DNA strands have lost part of their complementariness."}
{"id": "PMID:1081511", "title": "Rdiotoxicity of intracellular 67Ga, 125I and 3H. Nuclear versus cytoplasmic radiation effects in murine L1210 leukaemia.", "content": "L1210 leukaemia cells were labelled with various doses of 67Ga-citrate, 3H-thymidine, or 125I-iododeoxyuridine to evaluate the cytocidal effects of the intracellular decay of the three radionuclides. Based on radioisotope incorporation data, cellular dimensions, and intracellular radioisotope distributions (3H and 125I intranuclear, 67Ga cytoplasmic) the rates of deposition of cellular, nuclear, and cytoplasmic energy were calculated. In terms of energy absorption/cell, 67Ga (LD50: 2250 keV/hr; 69 rad/hr) was much less toxic than either 3H (LD50: 325 keV/hr; 10 rad/hr) of 125I (LD50:50 keV/hr; 1-5 rad/hr). In terms of energy absorption/nuclesu, 67Ga and 3H produced almost identical effects (LD50: 230 versus 255 keV/hr; 22-2 versus 24-6 rad/hr), but 125I remained much more toxic (LD50: 40 keV/hr; 3-9 rad/hr). These findings indicate that, although decay by electron capture in the cell nucleus (125I) is highly destructive, the same type of decay occurring in the cytoplasm (67Ga) is ineffective in killing L1210 cells. An analysis of the data suggests that the cytotoxic effects of the three radioisotopes result exclusively from nuclear damage. Cytoplasmic absorption of radiation energy appears to contribute little, if anything, to the lethal effects of ionizing radiations.", "contents": "Rdiotoxicity of intracellular 67Ga, 125I and 3H. Nuclear versus cytoplasmic radiation effects in murine L1210 leukaemia. L1210 leukaemia cells were labelled with various doses of 67Ga-citrate, 3H-thymidine, or 125I-iododeoxyuridine to evaluate the cytocidal effects of the intracellular decay of the three radionuclides. Based on radioisotope incorporation data, cellular dimensions, and intracellular radioisotope distributions (3H and 125I intranuclear, 67Ga cytoplasmic) the rates of deposition of cellular, nuclear, and cytoplasmic energy were calculated. In terms of energy absorption/cell, 67Ga (LD50: 2250 keV/hr; 69 rad/hr) was much less toxic than either 3H (LD50: 325 keV/hr; 10 rad/hr) of 125I (LD50:50 keV/hr; 1-5 rad/hr). In terms of energy absorption/nuclesu, 67Ga and 3H produced almost identical effects (LD50: 230 versus 255 keV/hr; 22-2 versus 24-6 rad/hr), but 125I remained much more toxic (LD50: 40 keV/hr; 3-9 rad/hr). These findings indicate that, although decay by electron capture in the cell nucleus (125I) is highly destructive, the same type of decay occurring in the cytoplasm (67Ga) is ineffective in killing L1210 cells. An analysis of the data suggests that the cytotoxic effects of the three radioisotopes result exclusively from nuclear damage. Cytoplasmic absorption of radiation energy appears to contribute little, if anything, to the lethal effects of ionizing radiations."}
{"id": "PMID:1081512", "title": "A comparative study of post-irradiation growth kinetics of spheroids and monolayers.", "content": "Post-irradiation growth kinetics of gamma-irradiated spheroid and monolayer cells in exponential growth phase was investigated by means of dose-response curves based on cell counts after specified time intervals following irradiation. A mathematical model of cell-growth after irradiation was fitted to these curves. The model parameters (related to division delay and growth of non-surviving cellsy obtained from this analysis consistently indicate increasing resistance to sub-lethal damage of cells cultured as multicellular spheroids under conditions of increasing three-dimensional contact. In contrast, no indication of an increased radiation-resistance was found with cells cultured on a substratum under a variety of conditions.", "contents": "A comparative study of post-irradiation growth kinetics of spheroids and monolayers. Post-irradiation growth kinetics of gamma-irradiated spheroid and monolayer cells in exponential growth phase was investigated by means of dose-response curves based on cell counts after specified time intervals following irradiation. A mathematical model of cell-growth after irradiation was fitted to these curves. The model parameters (related to division delay and growth of non-surviving cellsy obtained from this analysis consistently indicate increasing resistance to sub-lethal damage of cells cultured as multicellular spheroids under conditions of increasing three-dimensional contact. In contrast, no indication of an increased radiation-resistance was found with cells cultured on a substratum under a variety of conditions."}
{"id": "PMID:1081519", "title": "Suppression and recovery of the immune response and CFU potential of mice during and after chronic low-dose-rate gamma-irradiation.", "content": "Chronic exposure of mice to 60Co gamma-rays at rates of 1-6 R/hr and 6 R/hr leads to profound immunosuppression, as demonstrated with the Jerne haemolysis-in-agar technique. The immunosuppressive effect is dose-rate dependent. Thus, significant reduction of the number of splenic antibody plaque-forming cells (PFC) to sheep red blood cells is seen after exposure to 1005 R at 1-6 R/hr; at the higher exposure rate of 6 R/hr, PFC suppression is seen after a total dose on only 468 R. Although recovery of the PFC response is possible after accumulated doses of 1005, 1579, and 2138 R at 6 R/hr and 5256 R at 1-6 R/hr, normal immunological capacity had not returned 120 days after irradiation. In contrast the recovery of splenic colony-forming units (CFU), as an expression of the haematological potential of chronically irradiated mice, is found to be much more rapid than the repair of the immune system. Thus, 10 days after irradiation, at a time when little or no recovery in immune competence is apparent, the spleen of the chronically irradiatee animal has a CFU capacity about 50 times greater than has that of a normal, unirradiated mouse. The CFU's in the chronically irradiated spleens were also shown to be functional by virtue of their ability to protect lethally irradiated animals from haemopoietic death.", "contents": "Suppression and recovery of the immune response and CFU potential of mice during and after chronic low-dose-rate gamma-irradiation. Chronic exposure of mice to 60Co gamma-rays at rates of 1-6 R/hr and 6 R/hr leads to profound immunosuppression, as demonstrated with the Jerne haemolysis-in-agar technique. The immunosuppressive effect is dose-rate dependent. Thus, significant reduction of the number of splenic antibody plaque-forming cells (PFC) to sheep red blood cells is seen after exposure to 1005 R at 1-6 R/hr; at the higher exposure rate of 6 R/hr, PFC suppression is seen after a total dose on only 468 R. Although recovery of the PFC response is possible after accumulated doses of 1005, 1579, and 2138 R at 6 R/hr and 5256 R at 1-6 R/hr, normal immunological capacity had not returned 120 days after irradiation. In contrast the recovery of splenic colony-forming units (CFU), as an expression of the haematological potential of chronically irradiated mice, is found to be much more rapid than the repair of the immune system. Thus, 10 days after irradiation, at a time when little or no recovery in immune competence is apparent, the spleen of the chronically irradiatee animal has a CFU capacity about 50 times greater than has that of a normal, unirradiated mouse. The CFU's in the chronically irradiated spleens were also shown to be functional by virtue of their ability to protect lethally irradiated animals from haemopoietic death."}
{"id": "PMID:1081520", "title": "The effects of dose and field size on late radiation damage to the rat spinal cord.", "content": "In groups of young adult rats, 1, 2, 4, 8 or 16 mm lengths of the cervical spinal cord were irradiated with 2000 or 4000 R of 60Co gamma-rays. All groups showed significant life-shortening, all but five animals (which died from intercurrent disease) showing signs attributable to radiation. The clinical signs, latent period, and histological changes observed were dependent on the dose and field size. The animals irradiated with the higher dose to 8 or 16 mm of the cord were the first to die, all had developed paralysis and were found to have extensive areas of necrosis in the white matter. When the field size was decreased using this dose (4000 R), or the dose reduced (all field sizes), no extensive necrosis was found and only a proportion of the animals developed paralysis; the others died suddenly. The interpretation of these results is discussed.", "contents": "The effects of dose and field size on late radiation damage to the rat spinal cord. In groups of young adult rats, 1, 2, 4, 8 or 16 mm lengths of the cervical spinal cord were irradiated with 2000 or 4000 R of 60Co gamma-rays. All groups showed significant life-shortening, all but five animals (which died from intercurrent disease) showing signs attributable to radiation. The clinical signs, latent period, and histological changes observed were dependent on the dose and field size. The animals irradiated with the higher dose to 8 or 16 mm of the cord were the first to die, all had developed paralysis and were found to have extensive areas of necrosis in the white matter. When the field size was decreased using this dose (4000 R), or the dose reduced (all field sizes), no extensive necrosis was found and only a proportion of the animals developed paralysis; the others died suddenly. The interpretation of these results is discussed."}
{"id": "PMID:1081521", "title": "The modification of sodium excretion after abdominal irradiation. A dose-effect relationship study in the rat.", "content": "Whole-body counting of radiosodium disappearance in the rat enabled us to evaluate the disturbance induced in sodium excretion after gamma abdominal irradiation at different doses (500, 750, 1000, 1250 and 1500 R). The observed modification is consistent with a sodium excretion loss depending on the dose. Two indices, the relative excretion rate (theta) and the recovery time (deltat), completely define the radio-induced modification and make feasible the quantitative assessment of its dependence on the dose. The development of the dose-response relationship established for each of these indices leads us to conclude that the reduction in the sodium-excretion rate, which can be related to a defect in sodium intake, is a linear function of the dose, while the time needed to recover the pre-irradiation sodium excretion rate increases exponentially with the dose.", "contents": "The modification of sodium excretion after abdominal irradiation. A dose-effect relationship study in the rat. Whole-body counting of radiosodium disappearance in the rat enabled us to evaluate the disturbance induced in sodium excretion after gamma abdominal irradiation at different doses (500, 750, 1000, 1250 and 1500 R). The observed modification is consistent with a sodium excretion loss depending on the dose. Two indices, the relative excretion rate (theta) and the recovery time (deltat), completely define the radio-induced modification and make feasible the quantitative assessment of its dependence on the dose. The development of the dose-response relationship established for each of these indices leads us to conclude that the reduction in the sodium-excretion rate, which can be related to a defect in sodium intake, is a linear function of the dose, while the time needed to recover the pre-irradiation sodium excretion rate increases exponentially with the dose."}
{"id": "PMID:1081522", "title": "Interaction of ethidium bromide with heparin.", "content": "The reactions of hydrated electrons produced during pulse radiolysis have been utilized to investigate the binding of ethidium bromide to heparin. Complexes of ethidium bromide and heparin can be dissociated with salt. Divalent cations are more effective than monovalent cations in this respect. Pulse-radiolysis investigations at different temperatures indicate that the thermodynamic parameters governing the interaction of ethidium bromide with heparin are deltaH' = 11-6 kcal mole-1 and deltaS' = 42-6 cal deg-1 mole-1.", "contents": "Interaction of ethidium bromide with heparin. The reactions of hydrated electrons produced during pulse radiolysis have been utilized to investigate the binding of ethidium bromide to heparin. Complexes of ethidium bromide and heparin can be dissociated with salt. Divalent cations are more effective than monovalent cations in this respect. Pulse-radiolysis investigations at different temperatures indicate that the thermodynamic parameters governing the interaction of ethidium bromide with heparin are deltaH' = 11-6 kcal mole-1 and deltaS' = 42-6 cal deg-1 mole-1."}
{"id": "PMID:1081523", "title": "Pulse-radiolytic investigations of catechols and catecholamines. I. Adrenaline and adrenochrome.", "content": "Adrenaline (epinephrine), adrenochrome and C4-substituted catechol model compounds were pulse-irradiated in aqueous neutral and alkaline solutions. Transient spectra are reported after oxidizing adrenaline and reducing adrenochrome. All species appearing during 20 msec interval after the pulse have been identified: the OH adduct with an absorption maximum at 300-310 nm, the semiquinone (at 245 nm), and adrenaline quinone (at 340 nm). The reaction of superoxide anions (O2-) with adrenaline was less efficient, compared with OH radicals. A novel oxidation product, derived from the semiquinone and O2-, has been identified as the 4-hydroxy-3,6-dioxo derivate. The pulse-radiolytic reduction of adrenochrome by hydrated electrons (eaq-) yielded the semiquinone of adrenochrome (absorbing at 470 nm), which subsequently decays by a second-order process. The dismutation products leuco-adrenochrome (absorbing at 300 nm, pH 9-8) and the adrenochrome tautomer (absorbing at 375 nm) are unstable, forming 5,6-dihydro-N-methyl indole and regenerating adrenochrome.", "contents": "Pulse-radiolytic investigations of catechols and catecholamines. I. Adrenaline and adrenochrome. Adrenaline (epinephrine), adrenochrome and C4-substituted catechol model compounds were pulse-irradiated in aqueous neutral and alkaline solutions. Transient spectra are reported after oxidizing adrenaline and reducing adrenochrome. All species appearing during 20 msec interval after the pulse have been identified: the OH adduct with an absorption maximum at 300-310 nm, the semiquinone (at 245 nm), and adrenaline quinone (at 340 nm). The reaction of superoxide anions (O2-) with adrenaline was less efficient, compared with OH radicals. A novel oxidation product, derived from the semiquinone and O2-, has been identified as the 4-hydroxy-3,6-dioxo derivate. The pulse-radiolytic reduction of adrenochrome by hydrated electrons (eaq-) yielded the semiquinone of adrenochrome (absorbing at 470 nm), which subsequently decays by a second-order process. The dismutation products leuco-adrenochrome (absorbing at 300 nm, pH 9-8) and the adrenochrome tautomer (absorbing at 375 nm) are unstable, forming 5,6-dihydro-N-methyl indole and regenerating adrenochrome."}
{"id": "PMID:1081524", "title": "An investigation of chromosome damage in Vicia faba root tips after exposure to 1-5 MHz ultrasonic radiation.", "content": "Roots of seedlings of Vicia faba were exposed to 1-5 MHz continuous-wave ultrasound at approximately 5 W cm-2 intensity, averaged over the dividing region of the meristem, thus producing considerable non-thermal cell damage and severe inhibition of root growth. No significant increase in the number of coventional chromosome aberrations was observed during the 26 hours after exposure, although a significant number of cells did show bridged, agglomerated or clumped chromosomes. It is concluded that after exposure to ultrasound, genetic damage in surviving cells is unlikely.", "contents": "An investigation of chromosome damage in Vicia faba root tips after exposure to 1-5 MHz ultrasonic radiation. Roots of seedlings of Vicia faba were exposed to 1-5 MHz continuous-wave ultrasound at approximately 5 W cm-2 intensity, averaged over the dividing region of the meristem, thus producing considerable non-thermal cell damage and severe inhibition of root growth. No significant increase in the number of coventional chromosome aberrations was observed during the 26 hours after exposure, although a significant number of cells did show bridged, agglomerated or clumped chromosomes. It is concluded that after exposure to ultrasound, genetic damage in surviving cells is unlikely."}
{"id": "PMID:1081529", "title": "Storage of polyvinylpyrrolidone mimicking a congenital mucolipid storage disease in a patient with Munchausen's syndrome.", "content": "A 24-year-old woman from the Sinai peninsula had, since the age of 15 years, repeatedly undergone surgery because of gastrointestinal bleeding. The bleeding was found to have been caused by self-inflicted injuries. Histiocytes in the patient's liver and lymph nodes (the spleen had been removed nine years previously) contained a peculiar material which was, in part, basophilic and had puzzling histochemical characteristics. The material is believed to be polyvinylpyrrolidone (PVP), which had been administered to the patient during one of her operations. The importance of recognizing this change, especially in countries where PVP is administered as a plasma expander, is stressed.", "contents": "Storage of polyvinylpyrrolidone mimicking a congenital mucolipid storage disease in a patient with Munchausen's syndrome. A 24-year-old woman from the Sinai peninsula had, since the age of 15 years, repeatedly undergone surgery because of gastrointestinal bleeding. The bleeding was found to have been caused by self-inflicted injuries. Histiocytes in the patient's liver and lymph nodes (the spleen had been removed nine years previously) contained a peculiar material which was, in part, basophilic and had puzzling histochemical characteristics. The material is believed to be polyvinylpyrrolidone (PVP), which had been administered to the patient during one of her operations. The importance of recognizing this change, especially in countries where PVP is administered as a plasma expander, is stressed."}
{"id": "PMID:1081530", "title": "Specific effect of 5,6-dihydroxytryptamine on the monoamine fluorophore of the frog's gustatory cells.", "content": "A specific formaldehyde-induced yellow fluorescence, suggesting the presence of serotonin-like monoamine has been demonstrated in the gustatory cells of the frog. The fungiform papillae of frogs were examined fluorescence-histochemically after intraperitoneal injection of 5,6-dihydroxytryptamine. The results indicated that the fluorophore of gustatory cells was affected selectively by the drug injection: the yellow fluorescence was transiently enhanced 3 hours after the drug injection, thereafter being reduced rapidly. The effect of 5,6-dihydroxytryptamine was long-lasting with the reduction of the yellow fluorophore persisting at least for the experimental duration of 14 days. A single injection of 6-hydroxydopamine induced a complete depletion of noradrenaline fluorescence from adrenergic nerve terminals, while the fluorescence of gustatory cells was not affected by a high dose of the drug. The present results with pharmacologic treatments further support the view that the gustatory cell of the frog contains a serotonin-like monoamine.", "contents": "Specific effect of 5,6-dihydroxytryptamine on the monoamine fluorophore of the frog's gustatory cells. A specific formaldehyde-induced yellow fluorescence, suggesting the presence of serotonin-like monoamine has been demonstrated in the gustatory cells of the frog. The fungiform papillae of frogs were examined fluorescence-histochemically after intraperitoneal injection of 5,6-dihydroxytryptamine. The results indicated that the fluorophore of gustatory cells was affected selectively by the drug injection: the yellow fluorescence was transiently enhanced 3 hours after the drug injection, thereafter being reduced rapidly. The effect of 5,6-dihydroxytryptamine was long-lasting with the reduction of the yellow fluorophore persisting at least for the experimental duration of 14 days. A single injection of 6-hydroxydopamine induced a complete depletion of noradrenaline fluorescence from adrenergic nerve terminals, while the fluorescence of gustatory cells was not affected by a high dose of the drug. The present results with pharmacologic treatments further support the view that the gustatory cell of the frog contains a serotonin-like monoamine."}
{"id": "PMID:1081531", "title": "[Bleeding disorders and childhood ENT diseases (author's transl)].", "content": "The diagnosis and management of ENT patients with coincidental bleeding dyscrasias presents special difficulties, which include diagnosis of the disorder, arrest of the bleeding when unexpected, and the conservative and surgical care of affected children with ENT diseases. The dyscrasias most often found in childhood are haemophilia, von Willibrand's disease and ideopathic thrombocytopenic purpura. The clinical presentation and course of these disorders are described. Present day treatment is effective in arrest of the haemorrhage such that a fatality can almost always be avoided. The close cooperation between specialist paediatricians and haematology departments in the handling of these patients is stressed.", "contents": "[Bleeding disorders and childhood ENT diseases (author's transl)]. The diagnosis and management of ENT patients with coincidental bleeding dyscrasias presents special difficulties, which include diagnosis of the disorder, arrest of the bleeding when unexpected, and the conservative and surgical care of affected children with ENT diseases. The dyscrasias most often found in childhood are haemophilia, von Willibrand's disease and ideopathic thrombocytopenic purpura. The clinical presentation and course of these disorders are described. Present day treatment is effective in arrest of the haemorrhage such that a fatality can almost always be avoided. The close cooperation between specialist paediatricians and haematology departments in the handling of these patients is stressed."}
{"id": "PMID:1081528", "title": "Vasocilators in the canine mesenteric circulation. Evaluation of a potential aid in the diagnosis of gastrointestinal bleeding.", "content": "Radiodiagnostic potential of intra-arterially injected vasodialting agents was investigated by their effect on total and segmenal resistances (VR) of mesenteric vasculature, blood flow in superior mesenteric artery and its bleeding branch; heart rate and ventricular and systemic blood pressure. Dipyridamole, isoxsuprine, protricular and systemic blood pressure. Dipyridamole, isoxsuprine, prochlorperazine, lidocaine, meglumine diatrizoate and carbon dioxide were poor dilators. Phentolamine produced hypotension; glucagon and serpasil an extremely long dilation. A large and short vasodilation was produced with tolazoline and nylidrin, but both agents increased VR of the postcapillary segment and caused transient hypotension and arrhythmias, nylidrin's side effects were smaller. Oxygen produced large and long vasodilation and minimal systemic effects. It is concluded that oxygen or possibly nylidrin are suitable agents should an intermittently bleeding mesenteric artery be dilated for diagnostic purposes prior to angiography.", "contents": "Vasocilators in the canine mesenteric circulation. Evaluation of a potential aid in the diagnosis of gastrointestinal bleeding. Radiodiagnostic potential of intra-arterially injected vasodialting agents was investigated by their effect on total and segmenal resistances (VR) of mesenteric vasculature, blood flow in superior mesenteric artery and its bleeding branch; heart rate and ventricular and systemic blood pressure. Dipyridamole, isoxsuprine, protricular and systemic blood pressure. Dipyridamole, isoxsuprine, prochlorperazine, lidocaine, meglumine diatrizoate and carbon dioxide were poor dilators. Phentolamine produced hypotension; glucagon and serpasil an extremely long dilation. A large and short vasodilation was produced with tolazoline and nylidrin, but both agents increased VR of the postcapillary segment and caused transient hypotension and arrhythmias, nylidrin's side effects were smaller. Oxygen produced large and long vasodilation and minimal systemic effects. It is concluded that oxygen or possibly nylidrin are suitable agents should an intermittently bleeding mesenteric artery be dilated for diagnostic purposes prior to angiography."}
{"id": "PMID:1081532", "title": "Immunoglobulin-containing cells in pig mammary gland.", "content": "Peroxidase-labelled antisera to pig immunoglobulins A, G and M were used to study immunoglobulin-containing cell populations in pig mammary gland at different stages of gestation and lactation. Immunoglobulin-containing cells of all three classes were present several weeks before farrowing; IgA-containing cells were most numerous at all stages of lactation.", "contents": "Immunoglobulin-containing cells in pig mammary gland. Peroxidase-labelled antisera to pig immunoglobulins A, G and M were used to study immunoglobulin-containing cell populations in pig mammary gland at different stages of gestation and lactation. Immunoglobulin-containing cells of all three classes were present several weeks before farrowing; IgA-containing cells were most numerous at all stages of lactation."}
{"id": "PMID:1081535", "title": "Glycerol dissimilation in Rhodopseudomonas sphaeroides.", "content": "Rhodopseudomonas sphaeroides followed a diauxic growth curve when grown on a malate-glycerol medium, the first phase of growth being supported by malate and the second by glycerol. A soluble glycerokinase and a particulate, pyridine nucleotide-independent glycerophosphate dehydrogenase, were induced by the presence of glycerol in the medium, but neither was fully expressed nor functional until all malate had been consumed.", "contents": "Glycerol dissimilation in Rhodopseudomonas sphaeroides. Rhodopseudomonas sphaeroides followed a diauxic growth curve when grown on a malate-glycerol medium, the first phase of growth being supported by malate and the second by glycerol. A soluble glycerokinase and a particulate, pyridine nucleotide-independent glycerophosphate dehydrogenase, were induced by the presence of glycerol in the medium, but neither was fully expressed nor functional until all malate had been consumed."}
{"id": "PMID:1081536", "title": "Interaction between ethanolamine ammonia-lyase and methylcobalamin. Half-site reactivity with an adenosylcobalamin-dependent enzyme.", "content": "The adenosylcobalamin-dependent enzyme ethanolamine ammonia-lyase contains two active sites per molecule. The effects of methylcobalamin on the properties of this enzyme differ qualitatively depending on whether one or both of these sites is occupied by the cobamide. At 0.5 mol of methylcobalamin/mol of active sites, catalytic activity fell rapidly to approximately 30% of control levels, thereafter remaining constant for an hour. With the partially inhibited enzyme, Km values for ethanolamine and adenosylcobalamin were 5.5 muM and 1.6 muM, respectively, values that do not differ significantly from those of uninhibited enzyme. When the methylcobalamin per active site ratio was increased to 1, the decline in activity became progressive with time, eventually falling to levels much lower than seen at a cobamide per active site ratio of 0.5. Methylcobalamin also promotes the formation of a complex stable to gel filtration between ethanolamine and enzyme. Complex formation increased with increasing methylcobalamin per active site ratios up to a ratio of 0.7/1, at which point 0.5 mol of ethanol/mol of active sites was taken up. Ethanolamine uptake did not increase at higher methylcobalamin to active sites ratios. Methylcobalamin itself was taken up by enzyme, forming a complex containing 0.5 mol of methylcobalamin/mol of active sites that was stable to gel filtration. Measurement by the technique of Hummel and Dreyer ((1962) Biochim. Biophys. Acta 63, 530-532), however, showed one methylcobalamin binding site per active site. The formation of enzyme-ligand complexes stable to gel filtration was not affected by 5'-deoxyadenosine nor did 5'-deoxyadenosine by itself promote the formation of a stable complex between enzyme and ethanolamine. These observations were interpreted as evidence indicating half-site reactivity of ethanolamine ammonia-lyase with methylcobalamin. Comparison with previous results suggested that this half-site reactivity was an epiphenomenon not related to catalysis.", "contents": "Interaction between ethanolamine ammonia-lyase and methylcobalamin. Half-site reactivity with an adenosylcobalamin-dependent enzyme. The adenosylcobalamin-dependent enzyme ethanolamine ammonia-lyase contains two active sites per molecule. The effects of methylcobalamin on the properties of this enzyme differ qualitatively depending on whether one or both of these sites is occupied by the cobamide. At 0.5 mol of methylcobalamin/mol of active sites, catalytic activity fell rapidly to approximately 30% of control levels, thereafter remaining constant for an hour. With the partially inhibited enzyme, Km values for ethanolamine and adenosylcobalamin were 5.5 muM and 1.6 muM, respectively, values that do not differ significantly from those of uninhibited enzyme. When the methylcobalamin per active site ratio was increased to 1, the decline in activity became progressive with time, eventually falling to levels much lower than seen at a cobamide per active site ratio of 0.5. Methylcobalamin also promotes the formation of a complex stable to gel filtration between ethanolamine and enzyme. Complex formation increased with increasing methylcobalamin per active site ratios up to a ratio of 0.7/1, at which point 0.5 mol of ethanol/mol of active sites was taken up. Ethanolamine uptake did not increase at higher methylcobalamin to active sites ratios. Methylcobalamin itself was taken up by enzyme, forming a complex containing 0.5 mol of methylcobalamin/mol of active sites that was stable to gel filtration. Measurement by the technique of Hummel and Dreyer ((1962) Biochim. Biophys. Acta 63, 530-532), however, showed one methylcobalamin binding site per active site. The formation of enzyme-ligand complexes stable to gel filtration was not affected by 5'-deoxyadenosine nor did 5'-deoxyadenosine by itself promote the formation of a stable complex between enzyme and ethanolamine. These observations were interpreted as evidence indicating half-site reactivity of ethanolamine ammonia-lyase with methylcobalamin. Comparison with previous results suggested that this half-site reactivity was an epiphenomenon not related to catalysis."}
{"id": "PMID:1081537", "title": "An in vitro study of vitamin D2 hydroxylases in the chick.", "content": "An in vitro study of the liver 25-hydroxylation of vitamin D2 and the kidney 1- and 24-hydroxylations of 25-hydroxyvitamin D2 was undertaken in order to determine whether the discrimination against vitamin D2 seen in chicks in vivo is the result of a block of one or more of the steps in the activation of the vitamin D2 molecule. Vitamin D2 hydroxylation reactions in the chick are virtually identical with those observed with the vitamin D3 series. It is, therefore, concluded that the chick possesses the required enzymatic machinery to synthesize 1,25-dihydroxyvitamin D2 and that the discrimination must be because of some unknown metabolic reaction of the vitamin D2 compounds, to a defect in the transport of vitamin D2 metabolites, or to target organ discrimination.", "contents": "An in vitro study of vitamin D2 hydroxylases in the chick. An in vitro study of the liver 25-hydroxylation of vitamin D2 and the kidney 1- and 24-hydroxylations of 25-hydroxyvitamin D2 was undertaken in order to determine whether the discrimination against vitamin D2 seen in chicks in vivo is the result of a block of one or more of the steps in the activation of the vitamin D2 molecule. Vitamin D2 hydroxylation reactions in the chick are virtually identical with those observed with the vitamin D3 series. It is, therefore, concluded that the chick possesses the required enzymatic machinery to synthesize 1,25-dihydroxyvitamin D2 and that the discrimination must be because of some unknown metabolic reaction of the vitamin D2 compounds, to a defect in the transport of vitamin D2 metabolites, or to target organ discrimination."}
{"id": "PMID:1081538", "title": "Experimental correction of footdrop by electrical stimulation of the peroneal nerve.", "content": "Footdrop was corrected in thirteen of sixteen patients with centrally paralyzed ankle dorsiflexor muscles who were treated by electrical stimulation of the peroneal nerve by means of surgically implanted electrodes. Four patients required surgical revision before a good result was obtained. After follow-ups of more than three years there was no evidence of any nerve damage caused by chronic electrical stimulation of the peroneal nerve.", "contents": "Experimental correction of footdrop by electrical stimulation of the peroneal nerve. Footdrop was corrected in thirteen of sixteen patients with centrally paralyzed ankle dorsiflexor muscles who were treated by electrical stimulation of the peroneal nerve by means of surgically implanted electrodes. Four patients required surgical revision before a good result was obtained. After follow-ups of more than three years there was no evidence of any nerve damage caused by chronic electrical stimulation of the peroneal nerve."}
{"id": "PMID:1081541", "title": "Hemopoietic colony forming cells in regenerating mouse liver.", "content": "Cells from regenerating mouse liver removed 2-25 days post 68% hepatic resection have been assayed for in vivo colony forming capacity in soft agar (CFU-C), proliferative capacity in liquid culture, and in vivo spleen colony forming capacity (CFU-S). These studies demonstrated low concentrations of CFU-C and CFU-S in normal and sham operated liver, with an appreciable increase of both in regenerating liver, reaching maximum values in tissue removed 5--7 days post hepatic resection. Colony formation in agar by regenerating liver cells occurred in the absence of exogenous colony stimulating factor. Separation of liver cells on the basis of adherent properties prior to culture indicated concentration of CFU-C in the non-adherent fraction, while cells producing colony stimulating factor were concentrated in the adherent fraction.  Foci of actively dividing cells of themacrophage and granulocyte series arose in liquid culture from preparations of sham operated and regenerating liver, although total cell formation was greater with regenerating liver. A small proportion of the colonies formed in agar from regenerating liver consisted of cords of epithelioid cells, which resembled hepatocytes and differed from the macrophages or granulocytes found in the majority of colonies, raising the possibility that regenerating hepatocytes form colonies in agar culture.", "contents": "Hemopoietic colony forming cells in regenerating mouse liver. Cells from regenerating mouse liver removed 2-25 days post 68% hepatic resection have been assayed for in vivo colony forming capacity in soft agar (CFU-C), proliferative capacity in liquid culture, and in vivo spleen colony forming capacity (CFU-S). These studies demonstrated low concentrations of CFU-C and CFU-S in normal and sham operated liver, with an appreciable increase of both in regenerating liver, reaching maximum values in tissue removed 5--7 days post hepatic resection. Colony formation in agar by regenerating liver cells occurred in the absence of exogenous colony stimulating factor. Separation of liver cells on the basis of adherent properties prior to culture indicated concentration of CFU-C in the non-adherent fraction, while cells producing colony stimulating factor were concentrated in the adherent fraction.  Foci of actively dividing cells of themacrophage and granulocyte series arose in liquid culture from preparations of sham operated and regenerating liver, although total cell formation was greater with regenerating liver. A small proportion of the colonies formed in agar from regenerating liver consisted of cords of epithelioid cells, which resembled hepatocytes and differed from the macrophages or granulocytes found in the majority of colonies, raising the possibility that regenerating hepatocytes form colonies in agar culture."}
{"id": "PMID:1081542", "title": "Separation of subpopulations of in vitro colony forming cells from mouse marrow by equilibrium density centrifugation.", "content": "Equilibrium density centrifugation was used to characterise and separate subpopulations of mouse haemopoietic progenitor cells capable of producing colonies of granulocytes and macrophages in vitro. The material used to induce colony formation (CSF) was prepared from an extract of pregnant mouse uteri. This CSF preparation was found to be free of factors modifying the response. Under these culture conditions, in vitro colony forming cells (CFU-c) were found to be relatively homogeneous in their buoyant density. This homogeneity was independent of CSF concentration. A heterogeneous density profile of CFU-c was obtained when various cell fractions were cultured in the presence of CSF and rat blood lysate. The majority of the additional cells which responded to erythrocyte lysate were dense (modal density 1.080 g/cm3) compared to CFU-c which responded to CSF alone (modal density 1.074 g/cm3). It is concluded that in vitro colonies induced by CSF and in vitro colonies grown in the presence of CSF and erythrocyte lysate reflect two different populations of CFU-c.", "contents": "Separation of subpopulations of in vitro colony forming cells from mouse marrow by equilibrium density centrifugation. Equilibrium density centrifugation was used to characterise and separate subpopulations of mouse haemopoietic progenitor cells capable of producing colonies of granulocytes and macrophages in vitro. The material used to induce colony formation (CSF) was prepared from an extract of pregnant mouse uteri. This CSF preparation was found to be free of factors modifying the response. Under these culture conditions, in vitro colony forming cells (CFU-c) were found to be relatively homogeneous in their buoyant density. This homogeneity was independent of CSF concentration. A heterogeneous density profile of CFU-c was obtained when various cell fractions were cultured in the presence of CSF and rat blood lysate. The majority of the additional cells which responded to erythrocyte lysate were dense (modal density 1.080 g/cm3) compared to CFU-c which responded to CSF alone (modal density 1.074 g/cm3). It is concluded that in vitro colonies induced by CSF and in vitro colonies grown in the presence of CSF and erythrocyte lysate reflect two different populations of CFU-c."}
{"id": "PMID:1081543", "title": "Preparation and neutralization characteristics of an anti-CSF antibody.", "content": "Nine of ten rabbits immunized with a partially purified L-cell CSF had demonstrable titers of anti-CSF activity. In vitro the antibody was markedly inhibitory to both post-endotoxin mouse sera and several mouse tissue extracts. CSF containing conditioned media prepared from a number of sources showed variable inhibition suggesting that murine CSF's may be characterized by marked antigenic differences. Human sources of CSF were also inhibited thus indicating a degree of cross-reactivity between murine and human factors. These studies may provide the initial steps toward definition of the role of CSF in vivo.", "contents": "Preparation and neutralization characteristics of an anti-CSF antibody. Nine of ten rabbits immunized with a partially purified L-cell CSF had demonstrable titers of anti-CSF activity. In vitro the antibody was markedly inhibitory to both post-endotoxin mouse sera and several mouse tissue extracts. CSF containing conditioned media prepared from a number of sources showed variable inhibition suggesting that murine CSF's may be characterized by marked antigenic differences. Human sources of CSF were also inhibited thus indicating a degree of cross-reactivity between murine and human factors. These studies may provide the initial steps toward definition of the role of CSF in vivo."}
{"id": "PMID:1081539", "title": "The value of moderate hypothermia during anoxic cardiac arrest for coronary artery surgery.", "content": "Anoxic cardiac arrest, as opposed to induced ventricular fibrillation, greatly facilitates accurate distal anastomosis in aortocoronary bypass surgery. In order to diminish the anoxic insult, general and topical hypothermia may be used. In an attempt to establish the value of moderate hypothermia during anoxic cardiac arrest two groups of patients were compared. In group I coronary artery bypass procedures were performed under normothermic conditions with anoxic cardiac arrest. Patients in group II underwent similar procedures but under hypothermic conditions. General body hypothermia to an esophageal temperature of 30 degrees C and topical hypothermia with iced saline lavage were used. Using these techniques, the average intramyocardial temperature was 26 degrees C. Nonfatal cardiac complications did not occur more frequently in the hypothermic group. Operative mortality was decreased from 6.3% in the normothermic group to 1.5% in the hypothermic group. However, in group II, in addition to hypothermia, a second factor in the reduction of mortality was the completeness of the revascularization procedure: 58.5% of the patients had three or more bypass grafts in the hypothermic group. The mean anoxic arrest time was over 50 min for all patients--those who survived as well as those who died with postoperative low cardiac output or myocardial infarction. Therefore, anoxic arrest time should be kept as short as possible and certainly less than 50 min. Intermittent aortic occlusion and performance of the proximal anastomoses using a partial occluding clamp on the aorta are currently being used and, together with moderate hypothermia, provide a further reduction in postoperative myocardial complications.", "contents": "The value of moderate hypothermia during anoxic cardiac arrest for coronary artery surgery. Anoxic cardiac arrest, as opposed to induced ventricular fibrillation, greatly facilitates accurate distal anastomosis in aortocoronary bypass surgery. In order to diminish the anoxic insult, general and topical hypothermia may be used. In an attempt to establish the value of moderate hypothermia during anoxic cardiac arrest two groups of patients were compared. In group I coronary artery bypass procedures were performed under normothermic conditions with anoxic cardiac arrest. Patients in group II underwent similar procedures but under hypothermic conditions. General body hypothermia to an esophageal temperature of 30 degrees C and topical hypothermia with iced saline lavage were used. Using these techniques, the average intramyocardial temperature was 26 degrees C. Nonfatal cardiac complications did not occur more frequently in the hypothermic group. Operative mortality was decreased from 6.3% in the normothermic group to 1.5% in the hypothermic group. However, in group II, in addition to hypothermia, a second factor in the reduction of mortality was the completeness of the revascularization procedure: 58.5% of the patients had three or more bypass grafts in the hypothermic group. The mean anoxic arrest time was over 50 min for all patients--those who survived as well as those who died with postoperative low cardiac output or myocardial infarction. Therefore, anoxic arrest time should be kept as short as possible and certainly less than 50 min. Intermittent aortic occlusion and performance of the proximal anastomoses using a partial occluding clamp on the aorta are currently being used and, together with moderate hypothermia, provide a further reduction in postoperative myocardial complications."}
{"id": "PMID:1081544", "title": "Control of granulopoiesis in man.III. inhibition of colony formation by dense leukocytes.", "content": "Cellular feeder layers, prepared from normal blood leukocytes, usually stimulate human marrow to form colonies. A significant increase in the stimulating activity of unseparated leukocyte feeder layers is brought about following the removal of dense leukocytes in a manner which avoids enrichment of any remaining cell type. Restoration of dense leukocytes to a dense leukocyte depleted leukocyte feeder layer results in the reduction of stimulating activity to that of an unseparated leukocyte feeder; however, addition of dense leukocytes to unseparated leukocyte feeder layers has no effect on the stimulatory activity, over the range of concentrations used in this study.", "contents": "Control of granulopoiesis in man.III. inhibition of colony formation by dense leukocytes. Cellular feeder layers, prepared from normal blood leukocytes, usually stimulate human marrow to form colonies. A significant increase in the stimulating activity of unseparated leukocyte feeder layers is brought about following the removal of dense leukocytes in a manner which avoids enrichment of any remaining cell type. Restoration of dense leukocytes to a dense leukocyte depleted leukocyte feeder layer results in the reduction of stimulating activity to that of an unseparated leukocyte feeder; however, addition of dense leukocytes to unseparated leukocyte feeder layers has no effect on the stimulatory activity, over the range of concentrations used in this study."}
{"id": "PMID:1081540", "title": "Emergency operations for left ventricular asynergy.", "content": "Thirty-two patients were submitted to an emergency surgical procedure for left ventricular asynergy, following myocardial infarction. Indications and results are analyzed. All patients were operated upon with the aid of cardiopulmonary by-pass; an aneurysmectomy was performed in twenty-nine patients, a plication technique in three. These procedures were combined with mitral and/or aortic replacement in five patients, closure of ventricular septal defect in five, aorto-coronary saphenous vein by-pass in five. Early over all post-operative mortality was 75%: 80% in patients operated within three months from myocardial infarction, 67% in patients operated after three months from myocardial infarction.", "contents": "Emergency operations for left ventricular asynergy. Thirty-two patients were submitted to an emergency surgical procedure for left ventricular asynergy, following myocardial infarction. Indications and results are analyzed. All patients were operated upon with the aid of cardiopulmonary by-pass; an aneurysmectomy was performed in twenty-nine patients, a plication technique in three. These procedures were combined with mitral and/or aortic replacement in five patients, closure of ventricular septal defect in five, aorto-coronary saphenous vein by-pass in five. Early over all post-operative mortality was 75%: 80% in patients operated within three months from myocardial infarction, 67% in patients operated after three months from myocardial infarction."}
{"id": "PMID:1081545", "title": "Failure of tritiated thymidine incorporation into DNA to reflect the autoradiographically demonstrable calcium-induced increase in thymic lymphoblast DNA synthesis.", "content": "Increasing the extracellular calcium concentration in thymic lymphocyte suspension from 0.6 to 1.8 mM stimulated the proliferation of the lymphoblast subpopulation as measured by increases in the proportion of cells autoradiographically labeled with 3H-TdR and in mitotic activity. However it was not possible to show this increased DNA synthesis by scintillometric measurement of the amount of 3H-TdR incorporated into extracted DNA. On the other hand, calcium did raise the incorporation of 14C-formate into the thymine residues of DNA, and increased the activity of isolated thymocyte thymidylate synthetase. In contrast to the mitogenic calcium ion, a thymidylate synthetase inhibitor, methotrexate, actually increased the incorporation of 3H-TdR into DNA. It is concluded that calcium increases the endogenous synthesis of thymidylate which in turn prevents the amount of incorporation of exogenous 3H-TdR from accurately reflecting the true level of DNA synthesis.", "contents": "Failure of tritiated thymidine incorporation into DNA to reflect the autoradiographically demonstrable calcium-induced increase in thymic lymphoblast DNA synthesis. Increasing the extracellular calcium concentration in thymic lymphocyte suspension from 0.6 to 1.8 mM stimulated the proliferation of the lymphoblast subpopulation as measured by increases in the proportion of cells autoradiographically labeled with 3H-TdR and in mitotic activity. However it was not possible to show this increased DNA synthesis by scintillometric measurement of the amount of 3H-TdR incorporated into extracted DNA. On the other hand, calcium did raise the incorporation of 14C-formate into the thymine residues of DNA, and increased the activity of isolated thymocyte thymidylate synthetase. In contrast to the mitogenic calcium ion, a thymidylate synthetase inhibitor, methotrexate, actually increased the incorporation of 3H-TdR into DNA. It is concluded that calcium increases the endogenous synthesis of thymidylate which in turn prevents the amount of incorporation of exogenous 3H-TdR from accurately reflecting the true level of DNA synthesis."}
{"id": "PMID:1081546", "title": "Colony-stimulating factor (CSF) in the uterus of the pregnant mouse.", "content": "During the growth of the pregnant uterus of inbred Balb/c mice uterine colony-stimulating factor (CSF) content increased 800-fold. This corresponded to a 75-fold increase in CSF concentration. The size of the increase was the same in the uteri of inbred Balb/c mice and the uteri of F1 hybrid C57Bl/Balb/c mice mated with F1 males. It was about four times greater in these uteri than it was in the uteri of randomly mated TO mice. After unilateral Fallopian tubal ligation of TO mice the concentration of CSF was as great in the non-gravid uterine horns as it was in the gravid uterine horns. Physicochemical characterization of CSF from extracts of pregnant uterus showed that it differed from that found in foetal extracts and that found in the circulation. Its function in the pregnant uterus is discussed.", "contents": "Colony-stimulating factor (CSF) in the uterus of the pregnant mouse. During the growth of the pregnant uterus of inbred Balb/c mice uterine colony-stimulating factor (CSF) content increased 800-fold. This corresponded to a 75-fold increase in CSF concentration. The size of the increase was the same in the uteri of inbred Balb/c mice and the uteri of F1 hybrid C57Bl/Balb/c mice mated with F1 males. It was about four times greater in these uteri than it was in the uteri of randomly mated TO mice. After unilateral Fallopian tubal ligation of TO mice the concentration of CSF was as great in the non-gravid uterine horns as it was in the gravid uterine horns. Physicochemical characterization of CSF from extracts of pregnant uterus showed that it differed from that found in foetal extracts and that found in the circulation. Its function in the pregnant uterus is discussed."}
{"id": "PMID:1081549", "title": "Identification of DNA-binding lymphocytes in patients with systemic lupus erythematosus.", "content": "Antigen-ginding lymphocytes capable of binding native DNA (DNA-ABC) were identified in the peripheral blood of normal controls and patients with systemic lupus erythematosus (SLE) by autoradiography with 125I-nDNA. 12 patients with active SLE had 404 +/- 273 (mean +/- SD) DNA-ABC/105 lymphocytes, while 7 inactive SLE patients and 13 normals had 120 +/- 48 and 48 +/- 36, respectively. All three groups were significantly different from one another (p less than 0.01). No significant correlation was detected between the quantity of anti-native DNA (nDNA) antibody and number of DNA-ABC; however, most patients with large amounts of anti-nDNA antibody had both active disease and large numbers of DNA-ABC. Numbers of DNA-ABC and lymphocytes with surface immunoglobulin (Ig) did not change significantly after an 18-h incubation at 37degreeC. After depletion of B-lymphocytes by passage over bead columns coated with a complex of IgG and anti-IgG, the great majority of DNA-ABC were removed in both normal subjects and SLE patients. Labeling lymphocytes sequentially with 125I-nDNA, followed by an indirect fluorescence technique for identification of surface Ig, indicated that the great mahority of radiolabeled cells had surface Ig by fluorescence microscopy in four normals (average 93%) and five patients with active SLF (average 82%). The predominance of nDNA-sensitive B-lymphocytes in the peripheral blood of both normals and SLE patients is consistent with the concept that the induction of the anti-nDNA antibody response is due to the stimulation of preexisting nDNA-specific B lymphocytes by mechanisms other than those necessarily involving participation of nDNA-specific T lymphocytes.", "contents": "Identification of DNA-binding lymphocytes in patients with systemic lupus erythematosus. Antigen-ginding lymphocytes capable of binding native DNA (DNA-ABC) were identified in the peripheral blood of normal controls and patients with systemic lupus erythematosus (SLE) by autoradiography with 125I-nDNA. 12 patients with active SLE had 404 +/- 273 (mean +/- SD) DNA-ABC/105 lymphocytes, while 7 inactive SLE patients and 13 normals had 120 +/- 48 and 48 +/- 36, respectively. All three groups were significantly different from one another (p less than 0.01). No significant correlation was detected between the quantity of anti-native DNA (nDNA) antibody and number of DNA-ABC; however, most patients with large amounts of anti-nDNA antibody had both active disease and large numbers of DNA-ABC. Numbers of DNA-ABC and lymphocytes with surface immunoglobulin (Ig) did not change significantly after an 18-h incubation at 37degreeC. After depletion of B-lymphocytes by passage over bead columns coated with a complex of IgG and anti-IgG, the great majority of DNA-ABC were removed in both normal subjects and SLE patients. Labeling lymphocytes sequentially with 125I-nDNA, followed by an indirect fluorescence technique for identification of surface Ig, indicated that the great mahority of radiolabeled cells had surface Ig by fluorescence microscopy in four normals (average 93%) and five patients with active SLF (average 82%). The predominance of nDNA-sensitive B-lymphocytes in the peripheral blood of both normals and SLE patients is consistent with the concept that the induction of the anti-nDNA antibody response is due to the stimulation of preexisting nDNA-specific B lymphocytes by mechanisms other than those necessarily involving participation of nDNA-specific T lymphocytes."}
{"id": "PMID:1081550", "title": "Thymus-dependent lymphocytes in human bone marrow.", "content": "Human bone marrow is known to contain significant numbers of bursa-dependent lymphocytes. The presence of thymus-dependent (T) cells is controversial. Bone marrow cells obtained from healthy volunteers was fractionated by density centrifugation. A lymphocte-enriched subpopulation was shown to be reactive to alloantigens in mixed lymphocyte culture and to contain substantial numbers of T lymphocytes. The T lymphocytes were detected by cell surface markers (rosette formation with sheep RBC) and by response to the mitogens phytohemagglutinin and concanavalin A. Bone marrow T cells exhibited functional characteristics quantitatively different from peripheral blood T cells, suggesting that they may represent a particular subpopulation of T cells. The lymphocyte-enriched fraction additionally contained committed granulopoietic stem cells capable of colony formation in semisolid gel. The presence of T cells in human bone marrow is consistent with findings in other mammals and may explain the high incidence of graft versus host disease in bone marrow transplant recipients.", "contents": "Thymus-dependent lymphocytes in human bone marrow. Human bone marrow is known to contain significant numbers of bursa-dependent lymphocytes. The presence of thymus-dependent (T) cells is controversial. Bone marrow cells obtained from healthy volunteers was fractionated by density centrifugation. A lymphocte-enriched subpopulation was shown to be reactive to alloantigens in mixed lymphocyte culture and to contain substantial numbers of T lymphocytes. The T lymphocytes were detected by cell surface markers (rosette formation with sheep RBC) and by response to the mitogens phytohemagglutinin and concanavalin A. Bone marrow T cells exhibited functional characteristics quantitatively different from peripheral blood T cells, suggesting that they may represent a particular subpopulation of T cells. The lymphocyte-enriched fraction additionally contained committed granulopoietic stem cells capable of colony formation in semisolid gel. The presence of T cells in human bone marrow is consistent with findings in other mammals and may explain the high incidence of graft versus host disease in bone marrow transplant recipients."}
{"id": "PMID:1081552", "title": "Autoradiographic studies of cerebellar histogenesis in the bullfrog tadpole during metamorphosis: the external granular layer.", "content": "Spontaneously metamorphosing bullfrog tadpoles and those induced to metamorphose by injections of thyroid stimulating hormone (TSH) were given a single intraperitoneal injection of thymidine-3H (10 muCi/g body weight). The brains were dissected out at various periods 3 hours to 14 days later, and processed for autoradiography. At the 3-hour interval after thymidine-3H injection, ependymal cells were labelled, but not the external granular layer (EGL) cells. Furthermore, in all the metamorphosing tadpoles intense labelling was restricted to the ependyma of the marginal region of the cerebellar plate. At 48 hours, labelled cells were seen in the EGL of the marginal region. At the 4-day interval, most of the EGL was labelled, and labelled cells were also seen migrating from the EGL into the internal granular layer (IGL). By 14 days, several labelled cells were seen in the IGL. Although the sequence of cerebellar histogenesis in the frog is similar to the general pattern described in other vertebrate groups, the results indicate that the EGL of the frog cerebellum does not serve the function of a secondary proliferating zone.", "contents": "Autoradiographic studies of cerebellar histogenesis in the bullfrog tadpole during metamorphosis: the external granular layer. Spontaneously metamorphosing bullfrog tadpoles and those induced to metamorphose by injections of thyroid stimulating hormone (TSH) were given a single intraperitoneal injection of thymidine-3H (10 muCi/g body weight). The brains were dissected out at various periods 3 hours to 14 days later, and processed for autoradiography. At the 3-hour interval after thymidine-3H injection, ependymal cells were labelled, but not the external granular layer (EGL) cells. Furthermore, in all the metamorphosing tadpoles intense labelling was restricted to the ependyma of the marginal region of the cerebellar plate. At 48 hours, labelled cells were seen in the EGL of the marginal region. At the 4-day interval, most of the EGL was labelled, and labelled cells were also seen migrating from the EGL into the internal granular layer (IGL). By 14 days, several labelled cells were seen in the IGL. Although the sequence of cerebellar histogenesis in the frog is similar to the general pattern described in other vertebrate groups, the results indicate that the EGL of the frog cerebellum does not serve the function of a secondary proliferating zone."}
{"id": "PMID:1081551", "title": "Effects of cytotoxic immunosuppressants on tuberculin-sensitive lymphocytes in guinea pigs.", "content": "The immunosuppressive activities of two phase-specific drugs, 6-mercaptopurine (6-MP) and methotrexate, and a cycle-specific agent, cyclophosphamide, were evaluated on the lymphocytic component of established tuberculin hypersensitivity in guinea pigs. In these animals, purified protein derivative (PPD)-sensitive lymphocytes are in an intermitotic phase of their proliferative cycle. Neither phase-specific drug significantly altered either the number or functional activities of these lymphocytes. By two in vitro criteria, PPD-induced lymphoproliferation and elaboration of migration inhibition factor (MIF), the responses of lymph node cells were equivalent to sensitized controls. In addition, these agents did not deplete pools of T lymphocytes, impair responses to phytohemagglutinin (PHA), nor inhibit cutaneous reactivity if employed before sensitization. In contrast, cyclophosphamide showed broader immunosuppressive effects including significant toxicities for intermitotic lymphocytes. This drug depleted pools of T cells and markedly impaired the in vitro proliferative responses of residual lymphocytes. The latter occurred with both PHA and PPD. Suppression of PHA reactivity was a dose-dependent phenomenon but was evident even with small quantities of this alkylating agent. The suppression of antigen-induced responses was independent of the proliferative status of target lymphocytes in vivo, after a single large dose, it persisted for more than 3 wk. In total, these results indicate that the effective use of cytotoxic drugs as immunosuppressants must include consideration of both the cycle specificities of the agent and the proliferative activities of the target lymphoid population.", "contents": "Effects of cytotoxic immunosuppressants on tuberculin-sensitive lymphocytes in guinea pigs. The immunosuppressive activities of two phase-specific drugs, 6-mercaptopurine (6-MP) and methotrexate, and a cycle-specific agent, cyclophosphamide, were evaluated on the lymphocytic component of established tuberculin hypersensitivity in guinea pigs. In these animals, purified protein derivative (PPD)-sensitive lymphocytes are in an intermitotic phase of their proliferative cycle. Neither phase-specific drug significantly altered either the number or functional activities of these lymphocytes. By two in vitro criteria, PPD-induced lymphoproliferation and elaboration of migration inhibition factor (MIF), the responses of lymph node cells were equivalent to sensitized controls. In addition, these agents did not deplete pools of T lymphocytes, impair responses to phytohemagglutinin (PHA), nor inhibit cutaneous reactivity if employed before sensitization. In contrast, cyclophosphamide showed broader immunosuppressive effects including significant toxicities for intermitotic lymphocytes. This drug depleted pools of T cells and markedly impaired the in vitro proliferative responses of residual lymphocytes. The latter occurred with both PHA and PPD. Suppression of PHA reactivity was a dose-dependent phenomenon but was evident even with small quantities of this alkylating agent. The suppression of antigen-induced responses was independent of the proliferative status of target lymphocytes in vivo, after a single large dose, it persisted for more than 3 wk. In total, these results indicate that the effective use of cytotoxic drugs as immunosuppressants must include consideration of both the cycle specificities of the agent and the proliferative activities of the target lymphoid population."}
{"id": "PMID:1081553", "title": "Visual stimulation: effects on vestibular habituation.", "content": "The influence of various forms of visual stimulation presented during the course of vestibular habituation to a caloric stimulus was studied. Eye movements which were either complementary or in opposition to the induced vestibular nystagmus were produced with an optokinetic drum. In addition, the effect of visual fixation during vestibular-response periods was studied. In all cases, the cats that received visual stimulation during the majority of the caloric trials habituated more slowly than did animals that received all the habituation trials in total darkness. These data conflict with previous reports of vestibular-visual interactions. Possible explanations for the discrepancy include species differences, distraction provided by the visual stimuli, and the transfer of learning from the dark to light conditions.", "contents": "Visual stimulation: effects on vestibular habituation. The influence of various forms of visual stimulation presented during the course of vestibular habituation to a caloric stimulus was studied. Eye movements which were either complementary or in opposition to the induced vestibular nystagmus were produced with an optokinetic drum. In addition, the effect of visual fixation during vestibular-response periods was studied. In all cases, the cats that received visual stimulation during the majority of the caloric trials habituated more slowly than did animals that received all the habituation trials in total darkness. These data conflict with previous reports of vestibular-visual interactions. Possible explanations for the discrepancy include species differences, distraction provided by the visual stimuli, and the transfer of learning from the dark to light conditions."}
{"id": "PMID:1081555", "title": "Aging, terminal decline, and terminal drop.", "content": "Data from a 20-year longitudinal study of persons over 60 were analyzed by step-wise multiple regression to test for declines in function with age, for terminal decline (linear relationship to time before death), and for terminal drop (curvilinear relationship to time before death). There were no substanial terminal drop effects. All health measures had substantial declines with age, and the physician's physical function rating, as well as the individual's self-rating, showed additional terminal decline. Intelligence had a substantial decline with age and a small terminal decline. Most activities declined with age but had no significant terminal decline. Despite these declines in health, intelligence, and activities, there was little or no decline in satisfaction or adjustment.", "contents": "Aging, terminal decline, and terminal drop. Data from a 20-year longitudinal study of persons over 60 were analyzed by step-wise multiple regression to test for declines in function with age, for terminal decline (linear relationship to time before death), and for terminal drop (curvilinear relationship to time before death). There were no substanial terminal drop effects. All health measures had substantial declines with age, and the physician's physical function rating, as well as the individual's self-rating, showed additional terminal decline. Intelligence had a substantial decline with age and a small terminal decline. Most activities declined with age but had no significant terminal decline. Despite these declines in health, intelligence, and activities, there was little or no decline in satisfaction or adjustment."}
{"id": "PMID:1081556", "title": "Characteristics of the 'active rosette test'. I. Technical considerations of the test and comments.", "content": "Using normal subjects, it was found that the 'Active Rosette Test' is both reproducible and consistent if performed exactly as described by Wybran et al. (1972). Variations in incubation time, centrifugal force while sedimenting rosettes, resuspension time after centrifugation, SRBC lymphocyte ratio, and increasing concentrations of fetal calf serum all produced marked deviations in test results. Rosette inhibition studies using a specific anti-T cell antisera showed inhibition of 'active' and total rosettes at the same level, suggesting that there are no marked variations in surface T antigens among respective T cells. Unless differences in functional activities can be demonstrated between the 'active' rosette-forming cells and the other T cells, the test will be of an empiric nature.", "contents": "Characteristics of the 'active rosette test'. I. Technical considerations of the test and comments. Using normal subjects, it was found that the 'Active Rosette Test' is both reproducible and consistent if performed exactly as described by Wybran et al. (1972). Variations in incubation time, centrifugal force while sedimenting rosettes, resuspension time after centrifugation, SRBC lymphocyte ratio, and increasing concentrations of fetal calf serum all produced marked deviations in test results. Rosette inhibition studies using a specific anti-T cell antisera showed inhibition of 'active' and total rosettes at the same level, suggesting that there are no marked variations in surface T antigens among respective T cells. Unless differences in functional activities can be demonstrated between the 'active' rosette-forming cells and the other T cells, the test will be of an empiric nature."}
{"id": "PMID:1081558", "title": "Action of staphylococcal epidermolytic toxin on mouse skin: an electron microscopic study.", "content": "The ultrastructure of the skin of 3-day-old mice challenged with small doses of highly purified staphylococcal epidermolytic toxin was examined at various time intervals. Up to 130 min few changes were evident, but at this time wide gaps developed between cells in the horizontal planes of the stratum granulosum, and \"bubbles\" normally present in the intracellular spece were no longer apparent. Splitting of the desmosomes occurred after the development of distended intercellular spaces. After 20 hr, that is, in the \"healing\" phase, the appearance suggested that normal maturation of keratinocytes was altered. Also at this time a degree of cell separation was still apparent in the outermost actively maturing layer of the stratum granulosum. The proteinase inhibitor Trasylol was tested for its effect on the toxin when administered up to 45 min after challenge.", "contents": "Action of staphylococcal epidermolytic toxin on mouse skin: an electron microscopic study. The ultrastructure of the skin of 3-day-old mice challenged with small doses of highly purified staphylococcal epidermolytic toxin was examined at various time intervals. Up to 130 min few changes were evident, but at this time wide gaps developed between cells in the horizontal planes of the stratum granulosum, and \"bubbles\" normally present in the intracellular spece were no longer apparent. Splitting of the desmosomes occurred after the development of distended intercellular spaces. After 20 hr, that is, in the \"healing\" phase, the appearance suggested that normal maturation of keratinocytes was altered. Also at this time a degree of cell separation was still apparent in the outermost actively maturing layer of the stratum granulosum. The proteinase inhibitor Trasylol was tested for its effect on the toxin when administered up to 45 min after challenge."}
{"id": "PMID:1081562", "title": "Second ear stapedectomy--a continued controversy.", "content": "This paper presents the results of an investigation into the effects of stapedectomy on vestibular function. We detected a disturbingly high incidence of vestibular abnormality in the results of caloric tests at three months postoperatively. Surprisingly, this was not usually accompanied by concurrent vestibular symptoms and there was no evidence of cochlear dysfunction in most cases. Although the caloric response at 12 months postoperatively showed an improvement in most instances, the responses rarely became symmetrical. We believe that a long-term alteration in vestibular response is not uncommon following stapedectomy. Although most patients will compensate quite quickly after unilateral stapedectomy we suspect that this occurs much less readily after bilateral stapedectomy and not at all should bilateral impairment of vestibular function occur. On the basis of this evidence, we submit that bilateral stapedectomy is justified only when vestibular function can be shown to be normal prior to the second ear operation.", "contents": "Second ear stapedectomy--a continued controversy. This paper presents the results of an investigation into the effects of stapedectomy on vestibular function. We detected a disturbingly high incidence of vestibular abnormality in the results of caloric tests at three months postoperatively. Surprisingly, this was not usually accompanied by concurrent vestibular symptoms and there was no evidence of cochlear dysfunction in most cases. Although the caloric response at 12 months postoperatively showed an improvement in most instances, the responses rarely became symmetrical. We believe that a long-term alteration in vestibular response is not uncommon following stapedectomy. Although most patients will compensate quite quickly after unilateral stapedectomy we suspect that this occurs much less readily after bilateral stapedectomy and not at all should bilateral impairment of vestibular function occur. On the basis of this evidence, we submit that bilateral stapedectomy is justified only when vestibular function can be shown to be normal prior to the second ear operation."}
{"id": "PMID:1081563", "title": "Reversible hearing loss in tumours of the cerebello-pontine angle.", "content": "A survey of the literature concerning reversible hearing loss in tumours of the cerebello-pontine angle is presented together with two additional cases. After removal of one meningioma and one epidermoid tumour, respectively, the abolished acoustico-vestibular function returned in both cases. The pre-requisites for the restoration of hearing are discussed.", "contents": "Reversible hearing loss in tumours of the cerebello-pontine angle. A survey of the literature concerning reversible hearing loss in tumours of the cerebello-pontine angle is presented together with two additional cases. After removal of one meningioma and one epidermoid tumour, respectively, the abolished acoustico-vestibular function returned in both cases. The pre-requisites for the restoration of hearing are discussed."}
{"id": "PMID:1081574", "title": "The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor.", "content": "The responsiveness of purified Fc- and Fc+ T lymphocytes, isolated from normal spleen cell populations by cell sorting on the fluorescence activated cell sorter, has been examined. Although both Fc- and Fc+ T cells responded to phytohemagglutinin, the response to concanavalin A (Con A) was found to be a characteristic of the Fc+ T lymphocyte. The poor responsiveness of the Fc- T cells to Con A was shown not to be due to a requirement of either different concentrations of Con A or for adherent cells. The addition of Fc+ T cells to the Fc- T cells in a ratio of 1:3 resulted in a mitotic response not significantly different from that observed with the purified Fc+ T cells alone and up to 15-fold greater than that of Fc- T cells alone. It is suggested that the Fc T cells can be recruited into mitosis as a result of Con A stimulation of the Fc+ T cells.", "contents": "The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor. The responsiveness of purified Fc- and Fc+ T lymphocytes, isolated from normal spleen cell populations by cell sorting on the fluorescence activated cell sorter, has been examined. Although both Fc- and Fc+ T cells responded to phytohemagglutinin, the response to concanavalin A (Con A) was found to be a characteristic of the Fc+ T lymphocyte. The poor responsiveness of the Fc- T cells to Con A was shown not to be due to a requirement of either different concentrations of Con A or for adherent cells. The addition of Fc+ T cells to the Fc- T cells in a ratio of 1:3 resulted in a mitotic response not significantly different from that observed with the purified Fc+ T cells alone and up to 15-fold greater than that of Fc- T cells alone. It is suggested that the Fc T cells can be recruited into mitosis as a result of Con A stimulation of the Fc+ T cells."}
{"id": "PMID:1081575", "title": "In vitro cell-mediated immune responses to the male specific(H-Y) antigen in mice.", "content": "C57BL/10 female mice were primed to the male specific antigen H-Y, either by grafting with syngeneic male tail skin or by i.p. injection of syngeneic male spleen cells. Primed female spleen cells, either unseparated or filtered through nylon wool to remove most of the B lymphocytes, were then cultured for 5 days in vitro with irradiated syngeneic male spleen cells and assayed against 51Cr-labeled target cells. Both unseparated and nylon wool filtered female cells displayed significant cytotoxic activity restricted to male target cells. Pretreatment of sensitized female cells with antitheta serum and complement just before assay abolished cytotoxic responses. We were unable to demonstrate cell-mediated cytotoxic responses into two nonresponding strains, CBA and B10.A, which fail to reject male isografts. The cytotoxic activity of C57BL/10 female cells was restricted to male target cells histocompatible with C57BL/10 over at least a portion of the major (H-2) histocompatibility complex. We conclude that secondary in vitro cytotoxic responses against the H-Y antigen are mediated by cytotoxic T lymphocytes, and that the H-Y target cell antigen may be specified by the H-2 complex.", "contents": "In vitro cell-mediated immune responses to the male specific(H-Y) antigen in mice. C57BL/10 female mice were primed to the male specific antigen H-Y, either by grafting with syngeneic male tail skin or by i.p. injection of syngeneic male spleen cells. Primed female spleen cells, either unseparated or filtered through nylon wool to remove most of the B lymphocytes, were then cultured for 5 days in vitro with irradiated syngeneic male spleen cells and assayed against 51Cr-labeled target cells. Both unseparated and nylon wool filtered female cells displayed significant cytotoxic activity restricted to male target cells. Pretreatment of sensitized female cells with antitheta serum and complement just before assay abolished cytotoxic responses. We were unable to demonstrate cell-mediated cytotoxic responses into two nonresponding strains, CBA and B10.A, which fail to reject male isografts. The cytotoxic activity of C57BL/10 female cells was restricted to male target cells histocompatible with C57BL/10 over at least a portion of the major (H-2) histocompatibility complex. We conclude that secondary in vitro cytotoxic responses against the H-Y antigen are mediated by cytotoxic T lymphocytes, and that the H-Y target cell antigen may be specified by the H-2 complex."}
{"id": "PMID:1081576", "title": "Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.", "content": "An antigen-specific suppressive T-cell factor was extracted from physically disrupted thymocytes and spleen cells of mice that had been immunized with soluble protein antigens. The factor, when inoculated into syngeneic normal mice, could induce a significant suppression of IgG antibody response against a hapten coupled to the carrier protein by which the donor of the suppressor factor was immunized. The suppressor factor was found only effective in suppressing the antibody response of syngeneic or H-2 histocompatible recipients. The suppressive T-cell factor was removed by absorption with immunoadsorbent composed of the relevant antigen, but not with any of those of anti-immunoglobulin antibodies. The factor was successfully removed by alloantibodies with specificity for the K end (H-2K, I-A and I-B) of the H-2 complex of the donor strain, but not by those for the D end (I-C, SsSlp, and H-2D). The activity was removed by absorption with a heterologous antithymocyte serum. The mol wt of the suppression T-cell factor was between 35,000 and 60,000 as determined by Sephadex G-200 gel filtration. The suppressive T-cell factor was found to be a heat-liable protein.", "contents": "Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization. An antigen-specific suppressive T-cell factor was extracted from physically disrupted thymocytes and spleen cells of mice that had been immunized with soluble protein antigens. The factor, when inoculated into syngeneic normal mice, could induce a significant suppression of IgG antibody response against a hapten coupled to the carrier protein by which the donor of the suppressor factor was immunized. The suppressor factor was found only effective in suppressing the antibody response of syngeneic or H-2 histocompatible recipients. The suppressive T-cell factor was removed by absorption with immunoadsorbent composed of the relevant antigen, but not with any of those of anti-immunoglobulin antibodies. The factor was successfully removed by alloantibodies with specificity for the K end (H-2K, I-A and I-B) of the H-2 complex of the donor strain, but not by those for the D end (I-C, SsSlp, and H-2D). The activity was removed by absorption with a heterologous antithymocyte serum. The mol wt of the suppression T-cell factor was between 35,000 and 60,000 as determined by Sephadex G-200 gel filtration. The suppressive T-cell factor was found to be a heat-liable protein."}
{"id": "PMID:1081577", "title": "Abnormal ratio of membrane immunoglobulin classes in mice with an X-linked B-lymphocyte defect.", "content": "CBA/N mice have an X-linked genetic defect in B-lymphocyte function manifested by inability to make antibody responses to T-independent antigens. Plasma membrane immunoglobulin (Ig) on spleen, lymph node, and Peyer's patch cells was analyzed by lactoperoxidase-catalyzed iodination, NP-40 extraction, specific immunoprecipitation, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These studies indicated that the X-linked immune defect was associated, in all three cell types, with a decrease in the ratio of cell membrane IgD analog to cell membrane IgM. This suggests either that IgD analog may be important in initiation of T-independent antibody responses or that CBA/N mice lack a subpopulation of B cells specialized to respond to T-independent antigens, and that these cells are relatively rich in plasma membrane IgD analog.", "contents": "Abnormal ratio of membrane immunoglobulin classes in mice with an X-linked B-lymphocyte defect. CBA/N mice have an X-linked genetic defect in B-lymphocyte function manifested by inability to make antibody responses to T-independent antigens. Plasma membrane immunoglobulin (Ig) on spleen, lymph node, and Peyer's patch cells was analyzed by lactoperoxidase-catalyzed iodination, NP-40 extraction, specific immunoprecipitation, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These studies indicated that the X-linked immune defect was associated, in all three cell types, with a decrease in the ratio of cell membrane IgD analog to cell membrane IgM. This suggests either that IgD analog may be important in initiation of T-independent antibody responses or that CBA/N mice lack a subpopulation of B cells specialized to respond to T-independent antigens, and that these cells are relatively rich in plasma membrane IgD analog."}
{"id": "PMID:1081578", "title": "Fc-receptors, Ia-antigens, and immunoglobulin on normal and activated mouse T lymphocytes.", "content": "Using antibody coated bovine erythrocytes we were unable to demonstrate Fc-receptors on either thymus cells or T cells prepared from lymph node cell suspensions by anti-Ig column filtration. However, if parental thymus or lymph node T cells were transferred to X-irradiated F1 hybrids, activated donor T cells recovered from the recipient's spleen (ATC-spleen) were shown to express Fc-receptors. Fc-receptors were also demonstrable on ATC-spleen prepared between strain combinations differing at the M-locus. In marked contrast, Fc-receptors were not detected on ATC recovered from thoracic duct lymph (T.TDL). This applied to (a) H-2-activated T.TDL derived from normal thymus cells, (b) H-2-activated T.TDL derived from thymus cells depleted of B cells, and (c) M-locus-activated T.TDL. Of these three populations, surface Ig (of B cell origin) was detected on a large proportion of the first but not on the second and third populations. Thus, the failure to detect Fc-receptors on any of these populations could not be attributed to blocking by adsorbed surface Ig. In addition, various T-cell populations were examined by a microcytotoxicity assay for the presence of cell surface Ia-antigens. 5--10% of the thymus cells, 20--30% of cortisone-resistant thymus cells, 60--70% of lymph node cells, and 60--80% of ATC-spleen and T.TDL showed Ia.", "contents": "Fc-receptors, Ia-antigens, and immunoglobulin on normal and activated mouse T lymphocytes. Using antibody coated bovine erythrocytes we were unable to demonstrate Fc-receptors on either thymus cells or T cells prepared from lymph node cell suspensions by anti-Ig column filtration. However, if parental thymus or lymph node T cells were transferred to X-irradiated F1 hybrids, activated donor T cells recovered from the recipient's spleen (ATC-spleen) were shown to express Fc-receptors. Fc-receptors were also demonstrable on ATC-spleen prepared between strain combinations differing at the M-locus. In marked contrast, Fc-receptors were not detected on ATC recovered from thoracic duct lymph (T.TDL). This applied to (a) H-2-activated T.TDL derived from normal thymus cells, (b) H-2-activated T.TDL derived from thymus cells depleted of B cells, and (c) M-locus-activated T.TDL. Of these three populations, surface Ig (of B cell origin) was detected on a large proportion of the first but not on the second and third populations. Thus, the failure to detect Fc-receptors on any of these populations could not be attributed to blocking by adsorbed surface Ig. In addition, various T-cell populations were examined by a microcytotoxicity assay for the presence of cell surface Ia-antigens. 5--10% of the thymus cells, 20--30% of cortisone-resistant thymus cells, 60--70% of lymph node cells, and 60--80% of ATC-spleen and T.TDL showed Ia."}
{"id": "PMID:1081579", "title": "A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.", "content": "A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate.", "contents": "A glycoprotein associated with the membrane fraction of human B but not T lymphocytes. A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate."}
{"id": "PMID:1081580", "title": "Functional characteristics of Peyer's patch cells. III. Carrier priming of T cells by antigen feeding.", "content": "Peyer's patch T cells may serve an important role in the interaction of the host with intraluminal gut antigens. Studies presented in this paper demonstrate that T cells in murine Peyer's patches can be carrier primed for helper function in the induction of an antihapten response by feeding antigen. Carrier priming was assessed by measuring the ability of Peyer's patch cells from mice fed heterologous erythrocytes to enhance an antitrinitrophenyl (TNP) response in vitro when added to normal Peyer's patch cells cultured with TNP coupled to the erythrocyte used for feeding. Priming of T helper cells in Peyer's patches was antigen specific and occurred when erythrocytes were administered orally but not when erythrocytes were injected intravenously or intraperitoneally. Murine Peyer's patches are naturally deficient in a cooperating accessory adherent cell type(s) required for B-cell induction to humoral antibody synthesis in vitro and antigen feeding does not result in significant induction of Peyer's patch B cells to humoral antibody synthesis in vivo. Since Peyer's patch T cells can be carrier-antigen primed for helper function in the absence of B-cell induction to humoral antibody synthesis, these studies may indicate that T-cell priming is less dependent than B-cell induction on cooperating accessory adherent cells.", "contents": "Functional characteristics of Peyer's patch cells. III. Carrier priming of T cells by antigen feeding. Peyer's patch T cells may serve an important role in the interaction of the host with intraluminal gut antigens. Studies presented in this paper demonstrate that T cells in murine Peyer's patches can be carrier primed for helper function in the induction of an antihapten response by feeding antigen. Carrier priming was assessed by measuring the ability of Peyer's patch cells from mice fed heterologous erythrocytes to enhance an antitrinitrophenyl (TNP) response in vitro when added to normal Peyer's patch cells cultured with TNP coupled to the erythrocyte used for feeding. Priming of T helper cells in Peyer's patches was antigen specific and occurred when erythrocytes were administered orally but not when erythrocytes were injected intravenously or intraperitoneally. Murine Peyer's patches are naturally deficient in a cooperating accessory adherent cell type(s) required for B-cell induction to humoral antibody synthesis in vitro and antigen feeding does not result in significant induction of Peyer's patch B cells to humoral antibody synthesis in vivo. Since Peyer's patch T cells can be carrier-antigen primed for helper function in the absence of B-cell induction to humoral antibody synthesis, these studies may indicate that T-cell priming is less dependent than B-cell induction on cooperating accessory adherent cells."}
{"id": "PMID:1081581", "title": "Synergistic and suppressive interactions among mouse T lymphocytes in the response to phytohemagglutinin.", "content": "A synergistic effect in the proliferative response to phytohemagglutinin (PHA) can be observed in cultures containing a mixture of mouse CBA/Ca lymph node cells (LNC) and syngeneic CBA/T6T6 thymocytes (ThC) when compared to cultures containing only one cell type. This effect was analyzed, at various days of culture and in LNC-ThC mixtures of different ratios, by comparing the origin of the cells in mitosis (detected by caryotypic analysis), the stimulation of DNA synthesis, the number of blasts, and the percentage of blasts labeled after pulses of [3H]thymidine (detected by autoradiography). The following conclusions were reached: (a) ThC are induced to proliferate by the presence of LNC, while they are almost unresponsive to PHA when cultured alone; and (b) the strongest \"synergistic\" effect is exerted on LNC, whose proliferation is markedly enhanced. Evidence is presented that this last effect is not specific to the presence of ThC, but results from a dilution of LNC which retards the time when the culture reaches a critical concentration of blasts, above which proliferation progressively stops. Thus, conditions of culture allowing the response to PHA of a low concentration of LNC leads to the most prolonged T-cell proliferation. These observations may be relevant to the types of T-cell interactions, \"synergistic\" or \"suppressive,\" occurring during in vitro or in vivo immune responses.", "contents": "Synergistic and suppressive interactions among mouse T lymphocytes in the response to phytohemagglutinin. A synergistic effect in the proliferative response to phytohemagglutinin (PHA) can be observed in cultures containing a mixture of mouse CBA/Ca lymph node cells (LNC) and syngeneic CBA/T6T6 thymocytes (ThC) when compared to cultures containing only one cell type. This effect was analyzed, at various days of culture and in LNC-ThC mixtures of different ratios, by comparing the origin of the cells in mitosis (detected by caryotypic analysis), the stimulation of DNA synthesis, the number of blasts, and the percentage of blasts labeled after pulses of [3H]thymidine (detected by autoradiography). The following conclusions were reached: (a) ThC are induced to proliferate by the presence of LNC, while they are almost unresponsive to PHA when cultured alone; and (b) the strongest \"synergistic\" effect is exerted on LNC, whose proliferation is markedly enhanced. Evidence is presented that this last effect is not specific to the presence of ThC, but results from a dilution of LNC which retards the time when the culture reaches a critical concentration of blasts, above which proliferation progressively stops. Thus, conditions of culture allowing the response to PHA of a low concentration of LNC leads to the most prolonged T-cell proliferation. These observations may be relevant to the types of T-cell interactions, \"synergistic\" or \"suppressive,\" occurring during in vitro or in vivo immune responses."}
{"id": "PMID:1081582", "title": "Genetic control of cutaneous basophil hypersensitivity.", "content": "CBH to DNP-GL could be elicited only in responder guinea pigs which possess the genetic ability to develop classic delayed type hypersensitivity to DNP-PLL, the response to which is governed by the same gene. Since the defect in nonresponder animals seems to reside at the level of their T cells and not B cells, these results lend support to the contention that CBH, as well as DH, is dependent on and probably mediated by T cells.", "contents": "Genetic control of cutaneous basophil hypersensitivity. CBH to DNP-GL could be elicited only in responder guinea pigs which possess the genetic ability to develop classic delayed type hypersensitivity to DNP-PLL, the response to which is governed by the same gene. Since the defect in nonresponder animals seems to reside at the level of their T cells and not B cells, these results lend support to the contention that CBH, as well as DH, is dependent on and probably mediated by T cells."}
{"id": "PMID:1081583", "title": "Studies on the role of the host immune response in recovery from Friend virus leukemia. II. Cell-mediated immunity.", "content": "Congenic mouse strains differing only at genes within the H-2 complex were found to have virus-specific cytotoxic effector cells in their spleens during or after recovery from Friend leukemia virus-induced splenomegaly. These effector cells were theta-positive T lymphocytes which functioned in vitro without help or inhibition by B lymphocytes or glass-adherent cells. The antigenic specificities recognized by the effector cells were viral-induced cellular antigens apparently different from those identified by serological techniques.", "contents": "Studies on the role of the host immune response in recovery from Friend virus leukemia. II. Cell-mediated immunity. Congenic mouse strains differing only at genes within the H-2 complex were found to have virus-specific cytotoxic effector cells in their spleens during or after recovery from Friend leukemia virus-induced splenomegaly. These effector cells were theta-positive T lymphocytes which functioned in vitro without help or inhibition by B lymphocytes or glass-adherent cells. The antigenic specificities recognized by the effector cells were viral-induced cellular antigens apparently different from those identified by serological techniques."}
{"id": "PMID:1081584", "title": "Behavioral adaptation in neonatal chicks following embryonic vestibular system rearrangement.", "content": "Micro-surgery was performed on chick embryos during the second day of incubation (45-50 hours). Either one otocyst was removed or was rotated 180 degrees and reimplanted. The chicks' behavior was studied for 1-13 weeks after hatching. The unilateral loss or rearrangement of vestibular (and auditory) input led initially to abnormal behavior patterns, usually consisting of poor righting reflexes, deficits in locomotion, balance and pecking accuracy, and a pronounced head nystagmus. However, in every case, these abnormalities were corrected within a few days after hatching, so that the operated animals became almost indistinguishable from normal controls. Histological evidence confirmed the extent of the loss of the vestibular and auditory structures and nuclear amalgams. The observed capacity of the neonatal chick to adapt to this sensory loss or disorientation is a rather rare example of infraprimate behavioral adjustment or modifiability, contrasting with the frequent demonstrations of behavioral rigidity in lower forms.", "contents": "Behavioral adaptation in neonatal chicks following embryonic vestibular system rearrangement. Micro-surgery was performed on chick embryos during the second day of incubation (45-50 hours). Either one otocyst was removed or was rotated 180 degrees and reimplanted. The chicks' behavior was studied for 1-13 weeks after hatching. The unilateral loss or rearrangement of vestibular (and auditory) input led initially to abnormal behavior patterns, usually consisting of poor righting reflexes, deficits in locomotion, balance and pecking accuracy, and a pronounced head nystagmus. However, in every case, these abnormalities were corrected within a few days after hatching, so that the operated animals became almost indistinguishable from normal controls. Histological evidence confirmed the extent of the loss of the vestibular and auditory structures and nuclear amalgams. The observed capacity of the neonatal chick to adapt to this sensory loss or disorientation is a rather rare example of infraprimate behavioral adjustment or modifiability, contrasting with the frequent demonstrations of behavioral rigidity in lower forms."}
{"id": "PMID:1081585", "title": "Activation of phosphorylase in frog muscle as determined by contractile activity.", "content": "The state of activation of phosphorylation in muscle has been reinvestigated by combining the extraction procedures of Danforth, Helmreich, and Cori with the low-temperature techniques of this laboratory. In resting frog muscle, the phosphorylase-alpha content is usually below detectability. Upon contractile activity in series of twitches, activation of phosphorylase beta to alpha took place, without activation of phosphorylase beta kinase as defined by the assay procedure. Two different experimental designs were used to examine the relation between phosphorylase activation and the myothermally determined energy turnover per twitch, and these showed, identically, that the enzyme activation is proportional to the energy per twitch.", "contents": "Activation of phosphorylase in frog muscle as determined by contractile activity. The state of activation of phosphorylation in muscle has been reinvestigated by combining the extraction procedures of Danforth, Helmreich, and Cori with the low-temperature techniques of this laboratory. In resting frog muscle, the phosphorylase-alpha content is usually below detectability. Upon contractile activity in series of twitches, activation of phosphorylase beta to alpha took place, without activation of phosphorylase beta kinase as defined by the assay procedure. Two different experimental designs were used to examine the relation between phosphorylase activation and the myothermally determined energy turnover per twitch, and these showed, identically, that the enzyme activation is proportional to the energy per twitch."}
{"id": "PMID:1081586", "title": "Effect of intracranial pressure of meglumine iothalamate ventriculography.", "content": "Intraventricular pressure was studied in 12 patients undergoing ventriculography with a water soluble positive contrast medium. Isovolumetric instillation of meglumine iothalamate into the lateral ventricles and the anterior part of the third ventricle caused only a small increase in ventricular fluid pressure (1.3 +/- 0.3 mmHg), but the pressure increased markedly (46.3 +/- 3.7 mmHg; P less than 0.001) when the contrast medium entered the posterior end of the third ventricle, aqueduct of Sylvius,, and fourth ventricle. This sharp increase in intracranial pressure could not be attributed solely to the postural changes or to alterations in arterial blood pressure. Possible mechanisms are discussed.", "contents": "Effect of intracranial pressure of meglumine iothalamate ventriculography. Intraventricular pressure was studied in 12 patients undergoing ventriculography with a water soluble positive contrast medium. Isovolumetric instillation of meglumine iothalamate into the lateral ventricles and the anterior part of the third ventricle caused only a small increase in ventricular fluid pressure (1.3 +/- 0.3 mmHg), but the pressure increased markedly (46.3 +/- 3.7 mmHg; P less than 0.001) when the contrast medium entered the posterior end of the third ventricle, aqueduct of Sylvius,, and fourth ventricle. This sharp increase in intracranial pressure could not be attributed solely to the postural changes or to alterations in arterial blood pressure. Possible mechanisms are discussed."}
{"id": "PMID:1081587", "title": "Computerized axial tomography: the normal EMI scan.", "content": "Computerized axial tomography using the EMI scanner as a new method of using x-rays in diagnosis. The technique displays intracranial and orbital structures in the transverse plane. The appearances of normal EMI Scans are described and correlated with cerebral and orbital anatomy seen in transverse section.", "contents": "Computerized axial tomography: the normal EMI scan. Computerized axial tomography using the EMI scanner as a new method of using x-rays in diagnosis. The technique displays intracranial and orbital structures in the transverse plane. The appearances of normal EMI Scans are described and correlated with cerebral and orbital anatomy seen in transverse section."}
{"id": "PMID:1081588", "title": "Surgical removal at arteriovenous malformations from the brain stem and cerebellopontine angle.", "content": "The author reports his surgical experience with five cases of arteriovenous malformation of the brain stem and cerebelloppontine angle causing multiple hemorrhages and severe neurological deficits. Surgical removal of the lesions had good results in four cases; there was one death.", "contents": "Surgical removal at arteriovenous malformations from the brain stem and cerebellopontine angle. The author reports his surgical experience with five cases of arteriovenous malformation of the brain stem and cerebelloppontine angle causing multiple hemorrhages and severe neurological deficits. Surgical removal of the lesions had good results in four cases; there was one death."}
{"id": "PMID:1081589", "title": "Chronic petrous pyramid abscess presenting as a cerebellopontine angle mass. Case report.", "content": "The authors present the case of a 5-year-old boy with a chronic petrous pyramid abscess and without a history of otitis media. Treatment by posterior fossa exploration and drainage with antibiotics was successful.", "contents": "Chronic petrous pyramid abscess presenting as a cerebellopontine angle mass. Case report. The authors present the case of a 5-year-old boy with a chronic petrous pyramid abscess and without a history of otitis media. Treatment by posterior fossa exploration and drainage with antibiotics was successful."}
{"id": "PMID:1081594", "title": "[Duodenal cirsoid aneurysm treated by embolization(author's transl)].", "content": "A cirsoid aneurysm of the artery to the second part of the duodenum was demonstrated by arteriography in a 31 year old man who had had two severe unexplained bouts of gastrointestinal hemorrhage. The diagnosis was confirmed by surgical intervention, and in order to avoid duodenopancreatectomy, the malformation was treated by embolization of the gastroduodenal artery during the operation.", "contents": "[Duodenal cirsoid aneurysm treated by embolization(author's transl)]. A cirsoid aneurysm of the artery to the second part of the duodenum was demonstrated by arteriography in a 31 year old man who had had two severe unexplained bouts of gastrointestinal hemorrhage. The diagnosis was confirmed by surgical intervention, and in order to avoid duodenopancreatectomy, the malformation was treated by embolization of the gastroduodenal artery during the operation."}
{"id": "PMID:1081601", "title": "Effect of cordycepin (3'-deoxyadenosine) on virus-specific RNA species synthesized in Newcastle disease virus-infected cells.", "content": "Cordycepin (3'-deoxyadenosine) has no effect on the size or relative proportions of Newcastle disease virus-specific 18-22S mRNA species nor on the amount or size of the polyadenylic acid associated with them. Cordycepin does, however, cause an inhibition of incorporation of [3H]uridine into 50S virus-specific RNA relative to 18-22S RNA. This inhibition is probably not a direct effect of the drug on the synthesis of 50S viral RNA. Like cycloheximide, another drug which inhibits 50S RNA accumulation in paramyxovirus-infected cells, cordycepin inhibits protein synthesis as measured by amino acid incorporation. It is likely that the inhibition of 50S RNA accumulation is a secondary effect of protein synthesis inhibition. This is supported by the finding that concentrations of cordycepin and cycloheximide, which inhibit protein synthesis to the same extent, have the same effect on the ratio of 50 to 18-22S virus-specific RNA.", "contents": "Effect of cordycepin (3'-deoxyadenosine) on virus-specific RNA species synthesized in Newcastle disease virus-infected cells. Cordycepin (3'-deoxyadenosine) has no effect on the size or relative proportions of Newcastle disease virus-specific 18-22S mRNA species nor on the amount or size of the polyadenylic acid associated with them. Cordycepin does, however, cause an inhibition of incorporation of [3H]uridine into 50S virus-specific RNA relative to 18-22S RNA. This inhibition is probably not a direct effect of the drug on the synthesis of 50S viral RNA. Like cycloheximide, another drug which inhibits 50S RNA accumulation in paramyxovirus-infected cells, cordycepin inhibits protein synthesis as measured by amino acid incorporation. It is likely that the inhibition of 50S RNA accumulation is a secondary effect of protein synthesis inhibition. This is supported by the finding that concentrations of cordycepin and cycloheximide, which inhibit protein synthesis to the same extent, have the same effect on the ratio of 50 to 18-22S virus-specific RNA."}
{"id": "PMID:1081602", "title": "Replication and persistence of measles virus in defined subpopulations of human leukocytes.", "content": "Replication of Edmonston strain measles virus was studied in several human lymphoblast lines, as well as in defined subpopulations of circulating human leukocytes. It was found that measles virus can productively infect T cells, B cells, and monocytes from human blood. These conclusions were derived from infectious center studies on segregated cell populations, as well as from ultrastructural analyses on cells labeled with specific markers. In contrast, mature polymorphonuclear cells failed to synthesize measles virus nucleocapsids even after infection at a relatively high multiplicity of infection. Measles virus replicated more efficiently in lymphocytes stimulated with mitogens than in unstimulated cells. However, both phytohemagglutinin and pokeweed mitogen had a negligible stimulatory effect on viral synthesis in purified populations of monocytes. In all instances the efficiency of measles virus replication by monocytes was appreciably less than that of mitogenically stimulated lymphocytes or of continuously culture lymphoblasts. Under standard conditions of infection, all of the surveyed lymphoblast lines produced equivalent amounts of measles virus regardless of the major histocompatibility (HL-A) haplotype. Hence, no evidence was found that the HL-A3,7 haplotype conferred either an advantage or disadvantage with respect to measles virus synthesis in an immunologically neutral environment. A persistent infection with measles virus could be established in both T and B lymphoblasts. The release of infectious virus from such persistently infected cells was stable over a period of several weeks and was approximately 100-fold less than peak viral titers obtained in each respective line after acute infection.", "contents": "Replication and persistence of measles virus in defined subpopulations of human leukocytes. Replication of Edmonston strain measles virus was studied in several human lymphoblast lines, as well as in defined subpopulations of circulating human leukocytes. It was found that measles virus can productively infect T cells, B cells, and monocytes from human blood. These conclusions were derived from infectious center studies on segregated cell populations, as well as from ultrastructural analyses on cells labeled with specific markers. In contrast, mature polymorphonuclear cells failed to synthesize measles virus nucleocapsids even after infection at a relatively high multiplicity of infection. Measles virus replicated more efficiently in lymphocytes stimulated with mitogens than in unstimulated cells. However, both phytohemagglutinin and pokeweed mitogen had a negligible stimulatory effect on viral synthesis in purified populations of monocytes. In all instances the efficiency of measles virus replication by monocytes was appreciably less than that of mitogenically stimulated lymphocytes or of continuously culture lymphoblasts. Under standard conditions of infection, all of the surveyed lymphoblast lines produced equivalent amounts of measles virus regardless of the major histocompatibility (HL-A) haplotype. Hence, no evidence was found that the HL-A3,7 haplotype conferred either an advantage or disadvantage with respect to measles virus synthesis in an immunologically neutral environment. A persistent infection with measles virus could be established in both T and B lymphoblasts. The release of infectious virus from such persistently infected cells was stable over a period of several weeks and was approximately 100-fold less than peak viral titers obtained in each respective line after acute infection."}
{"id": "PMID:1081603", "title": "Identification of complement-receptor lymphocytes (B cells) in lymph nodes and tumor infiltrates.", "content": "To monitor the distribution of lymphocyte sub-populations infiltrating tumors, a simplified modification of standard techniques was developed to identify the complement-receptor B lymphocytes in frozen tissue sections. This technique is based on binding of antibody-coated bovine erythrocytes by B lymphocytes in the presence of sub-hemolytic concentrations of mouse complement. B lymphocytes can be identified in frozen tissue sections by adherent sensitized bovine erythrocytes. The specificity of the technique for B lymphocytes was established by 1) absence of spontaneous rosette formation with bovine erythrocytes by human T lymphocytes, 2) specific binding of bovine erythrocyte-antibody-complement complexes to B cell regions of normal lymph nodes and spleen and 3) binding of erythrocyte-antibody-complement to a high proportion of chronic lymphocytic leukemia (a B cell leukemia) lymphocytes. Five transitional carcinomas studied had regions of mononuclear infiltration that were virtually devoid of complement-receptor lymphocytes. The results suggest that lymphocytes infiltrating bladder carcinomas may be predominantly T lymphocytes.", "contents": "Identification of complement-receptor lymphocytes (B cells) in lymph nodes and tumor infiltrates. To monitor the distribution of lymphocyte sub-populations infiltrating tumors, a simplified modification of standard techniques was developed to identify the complement-receptor B lymphocytes in frozen tissue sections. This technique is based on binding of antibody-coated bovine erythrocytes by B lymphocytes in the presence of sub-hemolytic concentrations of mouse complement. B lymphocytes can be identified in frozen tissue sections by adherent sensitized bovine erythrocytes. The specificity of the technique for B lymphocytes was established by 1) absence of spontaneous rosette formation with bovine erythrocytes by human T lymphocytes, 2) specific binding of bovine erythrocyte-antibody-complement complexes to B cell regions of normal lymph nodes and spleen and 3) binding of erythrocyte-antibody-complement to a high proportion of chronic lymphocytic leukemia (a B cell leukemia) lymphocytes. Five transitional carcinomas studied had regions of mononuclear infiltration that were virtually devoid of complement-receptor lymphocytes. The results suggest that lymphocytes infiltrating bladder carcinomas may be predominantly T lymphocytes."}
{"id": "PMID:1081630", "title": "[Melatonin biosynthesis in the mammalian retina in dependence of light adaptation (author's transl)].", "content": "1. The biosynthesis of melatonin was investigated in retinaextracts of bovine eyes in dependence of light adaptation of the animals. S-adenososyl (Methyl-14C) methionin and acetylserotonin were used as substrates. A higher melatonin-biosynthesis was found in the retina from animals which were adapted to lower illumination. 2. The effect of triamterene on the biosynthesis of melatonin in the retina was examined. An increase of melatonin was found in the presence of the diureticum, which is a pteridine compound, when retinas of animals which were kept under low illumination were investigated. The role of melatoninsynthesis in dark adaptation is discussed and it is pointed to a possible danger of blinding in patients which used triamterene. Furthermore it is suggested that triamterene also increases the melatoninsynthesis in the pineal gland and that this may possibly contribute to an understanding of the effect of the diurecticum.", "contents": "[Melatonin biosynthesis in the mammalian retina in dependence of light adaptation (author's transl)]. 1. The biosynthesis of melatonin was investigated in retinaextracts of bovine eyes in dependence of light adaptation of the animals. S-adenososyl (Methyl-14C) methionin and acetylserotonin were used as substrates. A higher melatonin-biosynthesis was found in the retina from animals which were adapted to lower illumination. 2. The effect of triamterene on the biosynthesis of melatonin in the retina was examined. An increase of melatonin was found in the presence of the diureticum, which is a pteridine compound, when retinas of animals which were kept under low illumination were investigated. The role of melatoninsynthesis in dark adaptation is discussed and it is pointed to a possible danger of blinding in patients which used triamterene. Furthermore it is suggested that triamterene also increases the melatoninsynthesis in the pineal gland and that this may possibly contribute to an understanding of the effect of the diurecticum."}
{"id": "PMID:1081631", "title": "Rabbit gut-associated lymphoid tissue. Major pathway for thoracic duct lymphocyte circulation.", "content": "The demonstration of a preponderance of T cells in the thoracic duct lymph of rabbits prompted us to initiate cytokinetic studies using both uridine- and thymidine-labeled thoracic duct lymphocytes (TDL). Rabbits received intravenous injections of 8 to 11 X 10(8) autologous or allogeneic TDL, 95 per cent of which had incorporated 3H-uridine during a 1-hour in vitro incubation. Autoradiographs of tissues collected 24 hours after injection of TDL failed to demonstrate any trapping of label in liver or in vivo reutilization of 3H-uridine. No differences in the distribution of labeled cells were noted between recipients of autologous and allogeneic TDL. The paracortical areas of lymph nodes and periarterial areas of splenic follicles contained many heavily labeled cells; these areas therefore appear to correspond to thymus-dependent areas as in other rodents. The tonsils contained densely packed sheets of labeled cells. In the tonsillar but not in other germinal centers, evenly distributed, lightly labeled cells were seen. Small clusters of heavily labeled cells were seen in the bronchus-associated lymphoid tissue. In the appendix, sacculus rotundus, and Peyer's patches (GALT) densely packed, heavily labeled cells were seen in the interfollicular areas; fewer, heavily labeled cells were scattered throughout the dome and corona of GALT. In the dome and corona, however, there were many lightly labeled cells. The germinal centers of GALT were lacking totally in uridine-labeled cells. Some 20 per cent of TDL collected 24 hours after injection of uridine-labeled TDL were labeled, thereby reflecting considerable recirculation of TDL. Injection of 24 to 48 X 10(6) thymidine-labeled immunoblasts, obtained from TDL incubated with 3H-thymidine in vitro, into autologous or allogeneic recipients killed 24 hours later, revealed heavily labeled cells in the intestinal lamina propria, the dome, corona, and interfollicular areas of GALT, as well as throughout the spleen, lymph nodes, and tonsils. There was a 2- to 3-fold higher concentration of labeled cells in the appendiceal and mesenteric lymph nodes than in respective popliteal nodes. The potential significance of this differential distribution together with the homing-circulation patterns of these cells in GALT are discussed with regard to differentiation of IgA-producing cells. It is concluded that rabbit GALT contains a substantial number of cells belonging to the pool of recirculating lymphocytes and that B and T cells in the thoracic duct may have different rates of uridine incorporation as has been shown in other rodents.", "contents": "Rabbit gut-associated lymphoid tissue. Major pathway for thoracic duct lymphocyte circulation. The demonstration of a preponderance of T cells in the thoracic duct lymph of rabbits prompted us to initiate cytokinetic studies using both uridine- and thymidine-labeled thoracic duct lymphocytes (TDL). Rabbits received intravenous injections of 8 to 11 X 10(8) autologous or allogeneic TDL, 95 per cent of which had incorporated 3H-uridine during a 1-hour in vitro incubation. Autoradiographs of tissues collected 24 hours after injection of TDL failed to demonstrate any trapping of label in liver or in vivo reutilization of 3H-uridine. No differences in the distribution of labeled cells were noted between recipients of autologous and allogeneic TDL. The paracortical areas of lymph nodes and periarterial areas of splenic follicles contained many heavily labeled cells; these areas therefore appear to correspond to thymus-dependent areas as in other rodents. The tonsils contained densely packed sheets of labeled cells. In the tonsillar but not in other germinal centers, evenly distributed, lightly labeled cells were seen. Small clusters of heavily labeled cells were seen in the bronchus-associated lymphoid tissue. In the appendix, sacculus rotundus, and Peyer's patches (GALT) densely packed, heavily labeled cells were seen in the interfollicular areas; fewer, heavily labeled cells were scattered throughout the dome and corona of GALT. In the dome and corona, however, there were many lightly labeled cells. The germinal centers of GALT were lacking totally in uridine-labeled cells. Some 20 per cent of TDL collected 24 hours after injection of uridine-labeled TDL were labeled, thereby reflecting considerable recirculation of TDL. Injection of 24 to 48 X 10(6) thymidine-labeled immunoblasts, obtained from TDL incubated with 3H-thymidine in vitro, into autologous or allogeneic recipients killed 24 hours later, revealed heavily labeled cells in the intestinal lamina propria, the dome, corona, and interfollicular areas of GALT, as well as throughout the spleen, lymph nodes, and tonsils. There was a 2- to 3-fold higher concentration of labeled cells in the appendiceal and mesenteric lymph nodes than in respective popliteal nodes. The potential significance of this differential distribution together with the homing-circulation patterns of these cells in GALT are discussed with regard to differentiation of IgA-producing cells. It is concluded that rabbit GALT contains a substantial number of cells belonging to the pool of recirculating lymphocytes and that B and T cells in the thoracic duct may have different rates of uridine incorporation as has been shown in other rodents."}
{"id": "PMID:1081633", "title": "The late effects of selected immunosuppressants on immunocompetence, disease incidence, and mean life-span. I. Humoral immune activity.", "content": "The effect of different immunosuppressive treatments during young adulthood or humoral immune competence late in life was determined. It was found that the marked reduction in humoral immune competence in aged mice is further compromised when severe insults are administered early in life. Thus, thymectomy, splenectomy, and sublethal X-irradiation produced lasting immunodepression as measured (1) in situ and (2) by the hemolysin, direct and indirect plaque forming cell responses of adoptively transferred spleen cells. In contrast, treatment with cyclophosphamide and cortisone acetate were without effect, indicating that drug-damaged cells of the immune system were replaced by competent cells during the course of time. Decrease in immune competence of aged thymectomized animals could not be correlated with a decrease in numbers of theta-bearing T or immunoglobulin receptor-bearing B lymphocytes. The significance of the observed unequal effects of these immunosuppressants on immune competence, as they relate to disease incidence and life expectancy, are dealt with in the third paper in this series.", "contents": "The late effects of selected immunosuppressants on immunocompetence, disease incidence, and mean life-span. I. Humoral immune activity. The effect of different immunosuppressive treatments during young adulthood or humoral immune competence late in life was determined. It was found that the marked reduction in humoral immune competence in aged mice is further compromised when severe insults are administered early in life. Thus, thymectomy, splenectomy, and sublethal X-irradiation produced lasting immunodepression as measured (1) in situ and (2) by the hemolysin, direct and indirect plaque forming cell responses of adoptively transferred spleen cells. In contrast, treatment with cyclophosphamide and cortisone acetate were without effect, indicating that drug-damaged cells of the immune system were replaced by competent cells during the course of time. Decrease in immune competence of aged thymectomized animals could not be correlated with a decrease in numbers of theta-bearing T or immunoglobulin receptor-bearing B lymphocytes. The significance of the observed unequal effects of these immunosuppressants on immune competence, as they relate to disease incidence and life expectancy, are dealt with in the third paper in this series."}
{"id": "PMID:1081656", "title": "Influence of L-3-methoxytyrosine on monoamine synthesis in rat brain.", "content": "Rats were injected i.p. with L-3-methoxytyrosine, 100 or 300 mg/kg. One h later brain, liver, heart and blood plasma were analyzed for catecholamines and their precursors. In brain Dopa as well as dopamine and noradrenaline levels were unchanged, while demethylation of L-3-methoxytyrosine might have occurred in peripheral organs since Dopa levels in liver and dopamine in heart were elevated. 3-Methoxytyramine could not be detected in brain and liver after treatment with L-3-methoxytyrosine. Monoamine synthesis in vivo was measured in whole brain by determining the accumulation of Dopa and 5-hydroxytryptophan 30 min after the i.p. injection of NSD 1015(3-hydroxybenzylhydrazine HC1, 100 mg/kg), an inhibitor of the aromatic amino acid decarboxylase. L-3-Methoxytyrosine attenuated the formation of Dopa and 5-hydroxytryptophan by about 25% in brain tissue. The effect was paralleled by a decrease in the brain concentration of tryptophan.", "contents": "Influence of L-3-methoxytyrosine on monoamine synthesis in rat brain. Rats were injected i.p. with L-3-methoxytyrosine, 100 or 300 mg/kg. One h later brain, liver, heart and blood plasma were analyzed for catecholamines and their precursors. In brain Dopa as well as dopamine and noradrenaline levels were unchanged, while demethylation of L-3-methoxytyrosine might have occurred in peripheral organs since Dopa levels in liver and dopamine in heart were elevated. 3-Methoxytyramine could not be detected in brain and liver after treatment with L-3-methoxytyrosine. Monoamine synthesis in vivo was measured in whole brain by determining the accumulation of Dopa and 5-hydroxytryptophan 30 min after the i.p. injection of NSD 1015(3-hydroxybenzylhydrazine HC1, 100 mg/kg), an inhibitor of the aromatic amino acid decarboxylase. L-3-Methoxytyrosine attenuated the formation of Dopa and 5-hydroxytryptophan by about 25% in brain tissue. The effect was paralleled by a decrease in the brain concentration of tryptophan."}
{"id": "PMID:1081657", "title": "Change in the proportion of T and B lymphocytes in human malignant neoplasia in relation to the clinical stage.", "content": "Formation of spontaneous (E--erythrocyte) and immune (EAC--erythrocyte, antibody, complement) rosettes of sheep red blood cells (SRBC) with peripheral blood T and B lymphocytes, respectively, was used for quantitative assessment of these lymphocytes populations in tumor patients and control subjects. Relative counts of T and B lymphocytes have been correlated with lymphocyte survival in short-term cultures using standard subtoxic doses of phytohemagglutinin (PHA). The mean values and SE in normal control subjects for E rosettes (T lymphocytes) were 72.8 +/- 1.2%, for EAC rosettes (B lymphocytes) 23.8 +/- 2.5% of lymphocyte population. The survival of lymphocytes in short-term cultures with PHA in control subjects was 55.3 +/- 1.1%. In tumor patients the counts of E, EAC rosettes and percentage of lymphocyte survival in short-term PHA cultures were dependent upon the clinical stage. In patients with localized tumors the values were similar to controls. In patients where dissemination of the tumorous process could be ascertained a decrease of T lymphocyte counts, an increase of B lymphocyte counts and a higher survival of peripheral blood lymphocytes in short-term cultures with PHA was found.", "contents": "Change in the proportion of T and B lymphocytes in human malignant neoplasia in relation to the clinical stage. Formation of spontaneous (E--erythrocyte) and immune (EAC--erythrocyte, antibody, complement) rosettes of sheep red blood cells (SRBC) with peripheral blood T and B lymphocytes, respectively, was used for quantitative assessment of these lymphocytes populations in tumor patients and control subjects. Relative counts of T and B lymphocytes have been correlated with lymphocyte survival in short-term cultures using standard subtoxic doses of phytohemagglutinin (PHA). The mean values and SE in normal control subjects for E rosettes (T lymphocytes) were 72.8 +/- 1.2%, for EAC rosettes (B lymphocytes) 23.8 +/- 2.5% of lymphocyte population. The survival of lymphocytes in short-term cultures with PHA in control subjects was 55.3 +/- 1.1%. In tumor patients the counts of E, EAC rosettes and percentage of lymphocyte survival in short-term PHA cultures were dependent upon the clinical stage. In patients with localized tumors the values were similar to controls. In patients where dissemination of the tumorous process could be ascertained a decrease of T lymphocyte counts, an increase of B lymphocyte counts and a higher survival of peripheral blood lymphocytes in short-term cultures with PHA was found."}
{"id": "PMID:1081658", "title": "Migration inhibition of peritoneal cells and PHA lymphocyte toxicity in rats during methylcholanthrene carcinogenesis.", "content": "Peritoneal exudate cells obtained from rats bearing primary methylcholanthrene-induced tumors were shown to be inhibited in their capillary tube migration capacity as compared to cells from nontreated control litter-mates. The survival of rat peripheral blood lymphocytes harvested from the same individuals were tested simultaneously in short-term cultures with a standard concentration of Phytohemagglutinin. Lymphocytes from tumor-bearing rats survived better than control lymphocytes in 24-hour cultures with Phytohemagglutinin. A correlation was found from a comparison of the results of these two methods. These findings are discussed with regard to the possibility of T cells depletion from peripheral lymphocyte population, together with the enrichment of this population with B cells in tumor-bearing individuals.", "contents": "Migration inhibition of peritoneal cells and PHA lymphocyte toxicity in rats during methylcholanthrene carcinogenesis. Peritoneal exudate cells obtained from rats bearing primary methylcholanthrene-induced tumors were shown to be inhibited in their capillary tube migration capacity as compared to cells from nontreated control litter-mates. The survival of rat peripheral blood lymphocytes harvested from the same individuals were tested simultaneously in short-term cultures with a standard concentration of Phytohemagglutinin. Lymphocytes from tumor-bearing rats survived better than control lymphocytes in 24-hour cultures with Phytohemagglutinin. A correlation was found from a comparison of the results of these two methods. These findings are discussed with regard to the possibility of T cells depletion from peripheral lymphocyte population, together with the enrichment of this population with B cells in tumor-bearing individuals."}
{"id": "PMID:1081659", "title": "[Glomerulonephritis and sarcoidosis: study of a case].", "content": "A case of rapidly progressing glomerulonephritis accompanied by sarcoidosis is presented. The immunological pathogenesis of the underlying disease was shown by the positivity of the reactions for anti-IgA and anti-betal C/beta 1 A globulins and the presence of thick deposits in the mesexential matrix and some parts of the thickened basal membrane. Since generalised sarcoidosis is regarded as the expression of an immunological reaction to an unknown, diffuse antigen, this type of glomerulonephritis may be associated with the particular immunopathological condition observed in sarcoidosis.", "contents": "[Glomerulonephritis and sarcoidosis: study of a case]. A case of rapidly progressing glomerulonephritis accompanied by sarcoidosis is presented. The immunological pathogenesis of the underlying disease was shown by the positivity of the reactions for anti-IgA and anti-betal C/beta 1 A globulins and the presence of thick deposits in the mesexential matrix and some parts of the thickened basal membrane. Since generalised sarcoidosis is regarded as the expression of an immunological reaction to an unknown, diffuse antigen, this type of glomerulonephritis may be associated with the particular immunopathological condition observed in sarcoidosis."}
{"id": "PMID:1081664", "title": "Thymus-dependent lymphocyte function in dimethylnitrosamine-induced renal carcinogenesis is not depressed.", "content": "As one measure of cell-mediated immunity, the blastogenic response of enriched spleen lymphocytes to phytohaemagglutinin (PHA) was assayed at sequential daily and weekly intervals following the administration of dimethylnitrosamine (DMN) as a single dose sufficient to induce renal tumours in 100% of surviving rats. PHA responsiveness of DMN-treated rats was not suppressed at any stage of tumour induction in contrast to rats which had been intentionally immunodepressed by neonatal thymectomy. It was concluded that DMN is unlikely to facilitate the cancer process by impairing thymus-dependent lymphocyte function.", "contents": "Thymus-dependent lymphocyte function in dimethylnitrosamine-induced renal carcinogenesis is not depressed. As one measure of cell-mediated immunity, the blastogenic response of enriched spleen lymphocytes to phytohaemagglutinin (PHA) was assayed at sequential daily and weekly intervals following the administration of dimethylnitrosamine (DMN) as a single dose sufficient to induce renal tumours in 100% of surviving rats. PHA responsiveness of DMN-treated rats was not suppressed at any stage of tumour induction in contrast to rats which had been intentionally immunodepressed by neonatal thymectomy. It was concluded that DMN is unlikely to facilitate the cancer process by impairing thymus-dependent lymphocyte function."}
{"id": "PMID:1081665", "title": "[Immunologic types of lymphocytes in acute lymphatic leukemia during remission (author's transl)].", "content": "The rates of B and T-cell-subpopulations of lymphocytes were examined on 15 children with acute lymphatic leukemia during the first complete remission under maintenance in the Pinkel VII scheme and in 6 further cases with modification of this scheme of treatment compared to normal children in the same age groups. Further on 4 children during a relapse, on 1 during the second remission, and on 3 during  long-term remissions. The most important result was a significant reduction of B cells during intensive maintenance therapy of the Pinkel scheme. In the few cases of long-term remissions a reduction of B cells was no longer observed.", "contents": "[Immunologic types of lymphocytes in acute lymphatic leukemia during remission (author's transl)]. The rates of B and T-cell-subpopulations of lymphocytes were examined on 15 children with acute lymphatic leukemia during the first complete remission under maintenance in the Pinkel VII scheme and in 6 further cases with modification of this scheme of treatment compared to normal children in the same age groups. Further on 4 children during a relapse, on 1 during the second remission, and on 3 during  long-term remissions. The most important result was a significant reduction of B cells during intensive maintenance therapy of the Pinkel scheme. In the few cases of long-term remissions a reduction of B cells was no longer observed."}
{"id": "PMID:1081666", "title": "T cell leukaemia in the rat: the pathophysiology.", "content": "A new transplantable lymphocytic leukaemia of the inbred Hooded Oxford strain of rat is described. Fewer than 10 cells will transfer to syngeneic recipients. Leukaemic cells bear the surface markers of thymus-derived (T) cells and recirculate from blood to lymph. In contrast to the usual thymic lymphomas of rodents the pathophysiology of the disease bears a close resemblance to human acute lymphoblastic leukaemia.", "contents": "T cell leukaemia in the rat: the pathophysiology. A new transplantable lymphocytic leukaemia of the inbred Hooded Oxford strain of rat is described. Fewer than 10 cells will transfer to syngeneic recipients. Leukaemic cells bear the surface markers of thymus-derived (T) cells and recirculate from blood to lymph. In contrast to the usual thymic lymphomas of rodents the pathophysiology of the disease bears a close resemblance to human acute lymphoblastic leukaemia."}
{"id": "PMID:1081671", "title": "Lymphocyte surface receptors in childhood acute lymphocytic leukemia.", "content": "B and T lymphocyte surface receptors were studied in the bone marrow and peripheral blood of 16 patients with childhood acute lymphocytic leukemia. T cell surface receptors were identified on the blasts of one patient. Increased percentages of T or B cell surface receptors were not found in the bone marrow of the other 15 patients. There was a negative correlation between the percentage of pathologic cells in the peripheral blood and the percentage of cells with T and B surface receptors. The results of this study indicate that childhood acute lymphocytic leukemia is a heterogeneous disease in terms of lymphocyte surface receptors and that the pathologic cells of the majority of patients with this disease cannot be identified as being of thymic or non-thymic origin.", "contents": "Lymphocyte surface receptors in childhood acute lymphocytic leukemia. B and T lymphocyte surface receptors were studied in the bone marrow and peripheral blood of 16 patients with childhood acute lymphocytic leukemia. T cell surface receptors were identified on the blasts of one patient. Increased percentages of T or B cell surface receptors were not found in the bone marrow of the other 15 patients. There was a negative correlation between the percentage of pathologic cells in the peripheral blood and the percentage of cells with T and B surface receptors. The results of this study indicate that childhood acute lymphocytic leukemia is a heterogeneous disease in terms of lymphocyte surface receptors and that the pathologic cells of the majority of patients with this disease cannot be identified as being of thymic or non-thymic origin."}
{"id": "PMID:1081672", "title": "Hemophilus influenzae b pericarditis in children.", "content": "Recent experience in the diagnosis and management of Hemophilus influenzae b pericarditis is described in five children. Anterior pericardectomy appears to be the preferred method of surgical drainage because it was associated with a shorter hospitalization than pericardiocentesis or closed or open pericardotomy and removed the risk of recurrent cardiac tamponade and constrictive pericarditis. Countercurrent immunoelectrophoresis of sera and pericardial fluid was used to rapidly identify the etiology of pericarditis in four of four patients tested. The observation that three children appeared to develop pericarditis in the absence of a contiguous infectious focus suggests that bacteremic seeding of the pericardium may be important in the pathogenesis of this disease.", "contents": "Hemophilus influenzae b pericarditis in children. Recent experience in the diagnosis and management of Hemophilus influenzae b pericarditis is described in five children. Anterior pericardectomy appears to be the preferred method of surgical drainage because it was associated with a shorter hospitalization than pericardiocentesis or closed or open pericardotomy and removed the risk of recurrent cardiac tamponade and constrictive pericarditis. Countercurrent immunoelectrophoresis of sera and pericardial fluid was used to rapidly identify the etiology of pericarditis in four of four patients tested. The observation that three children appeared to develop pericarditis in the absence of a contiguous infectious focus suggests that bacteremic seeding of the pericardium may be important in the pathogenesis of this disease."}
{"id": "PMID:1081673", "title": "Neonatal intracranial hemorrhage in premature infants.", "content": "Since the advent of modern methods of neonatal care, intracranial hemorrhage in premature infants, which is usually intraventricular, is probably not as uniformly fatal as generally admitted and the survivors are likely to develop post-hemorrhagic hydrocephalus. This paper is a retrospective study of 11 premature babies born between 1968 and 1972 and diagnosed as having hydrocephalus secondary to neonatal intracranial hemorrhage. Nine of these are still alive and two have died, one at 3 and one at 17 months of age. Eight underwent permanant surgical drainage of hydrocephalus. The perinatal history, laboratory data, clinical evolution, and neurological outcome were studied. Two groups of infants were identified; those with signs of acute neurological deterioration in the neonatal period who had the most severe neurological sequellae (in four cases, periventricular lesions in addition to hydrocephalus were seen on the air study and in one case confirmed at autopsy) and those showing no obvious neurological signs in the neonatal period. Of this latter group, two appear to be developing normally. The therapeutic implications of the findings are discussed.", "contents": "Neonatal intracranial hemorrhage in premature infants. Since the advent of modern methods of neonatal care, intracranial hemorrhage in premature infants, which is usually intraventricular, is probably not as uniformly fatal as generally admitted and the survivors are likely to develop post-hemorrhagic hydrocephalus. This paper is a retrospective study of 11 premature babies born between 1968 and 1972 and diagnosed as having hydrocephalus secondary to neonatal intracranial hemorrhage. Nine of these are still alive and two have died, one at 3 and one at 17 months of age. Eight underwent permanant surgical drainage of hydrocephalus. The perinatal history, laboratory data, clinical evolution, and neurological outcome were studied. Two groups of infants were identified; those with signs of acute neurological deterioration in the neonatal period who had the most severe neurological sequellae (in four cases, periventricular lesions in addition to hydrocephalus were seen on the air study and in one case confirmed at autopsy) and those showing no obvious neurological signs in the neonatal period. Of this latter group, two appear to be developing normally. The therapeutic implications of the findings are discussed."}
{"id": "PMID:1081677", "title": "Potassium accumulation in the perinodal space of frog myelinated axons.", "content": "1. Voltage clamp experiments were carried out on frog myelinated fibres to study the origin of the transient inward current occuring when the membrane is repolarized after long lasting depolarizing pulses (tail current denominated \"Ip\" by Frankenhaeuser). 2. The \"tail\" of inward current measured during repolarization after break of the depolarizing pulse is insensitive to external application of TTX, is abolished by external treatment with TEA or Cs and decreases when the outward K-current during the pulse is diminished. 3. The time course of the \"tail\" current is exponential. Its direction depends on the duration of the depolarizing pulse and on the membrane potential level at repolarization. 4. It is concluded that the tail of inward current during repolarization is carried by K-ions accumulated in the perinodal space during a depolarizing pulse. The data suggest that the tail reflects the time course of the restoration of the K-concentration to its initial level. The tail current itself contributes to this restoration depending on the Em value at repolarization. 5. It is shown that one of the two phenomenological models proposed by Frankenhaeuser and Hodgkin to account for the external potassium accumulation observed in the squid giant axon may be also applied to the Ranvier node. Assuming that the thickness of the space is 2900 A and that the K-permeability of the barrier is 0.019 cm/sec, it is possible to account for the observed changes in [K]0 during a long lasting depolarizing pulse. 6. The existence of such a barrier would introduce an electrical resistance in series with the nodal membrane of roughly 150 000 omega.", "contents": "Potassium accumulation in the perinodal space of frog myelinated axons. 1. Voltage clamp experiments were carried out on frog myelinated fibres to study the origin of the transient inward current occuring when the membrane is repolarized after long lasting depolarizing pulses (tail current denominated \"Ip\" by Frankenhaeuser). 2. The \"tail\" of inward current measured during repolarization after break of the depolarizing pulse is insensitive to external application of TTX, is abolished by external treatment with TEA or Cs and decreases when the outward K-current during the pulse is diminished. 3. The time course of the \"tail\" current is exponential. Its direction depends on the duration of the depolarizing pulse and on the membrane potential level at repolarization. 4. It is concluded that the tail of inward current during repolarization is carried by K-ions accumulated in the perinodal space during a depolarizing pulse. The data suggest that the tail reflects the time course of the restoration of the K-concentration to its initial level. The tail current itself contributes to this restoration depending on the Em value at repolarization. 5. It is shown that one of the two phenomenological models proposed by Frankenhaeuser and Hodgkin to account for the external potassium accumulation observed in the squid giant axon may be also applied to the Ranvier node. Assuming that the thickness of the space is 2900 A and that the K-permeability of the barrier is 0.019 cm/sec, it is possible to account for the observed changes in [K]0 during a long lasting depolarizing pulse. 6. The existence of such a barrier would introduce an electrical resistance in series with the nodal membrane of roughly 150 000 omega."}
{"id": "PMID:1081678", "title": "Transient current changes and Na compartimentalization in frog skin epithelium.", "content": "Experimental conditions are described in which transient and positive current responses across isolated frog skin epithelia can be elicited by sudden addition of Na and Li ions (2--40 mM) in the outer bathing solutions. Subsequent return to outer Na (or Li) free conditions produce similar transient current changes but in the opposite direction. Analysis of the curve responses shows that the transient component of each curve is best described by a single, fast exponential term equation in case of Na addition to preparation unpoisonned with ouabain. In contrast, an equation including two exponential terms (a fast and a slow one) is required to fit the curve responses observed across ouabain treated epithelia or if Li is added outside. The transient responses were not significantly altered by substituying Cl for SO4(2)-anions. They were completely prevented by Amiloride (5-10(-5) M), increased by oxytocin (20 mU/ml) and markedly dependent upon the outer Na concentration. Interpreted in term of compartmental analysis, these observations suggest that a) the frog skin epithelium contains 2 separated but communicating compartments having different degrees of accessibility from outside; b) only that compartment filling at a fast rate (0.5 min) is involved in the transepithelial Na transport; c) the other one, filling at a rate of 4 to 7 min, is resplenished only under conditions where the basal pump system has a reduced activity. Tentative localization of these compartment is proposed.", "contents": "Transient current changes and Na compartimentalization in frog skin epithelium. Experimental conditions are described in which transient and positive current responses across isolated frog skin epithelia can be elicited by sudden addition of Na and Li ions (2--40 mM) in the outer bathing solutions. Subsequent return to outer Na (or Li) free conditions produce similar transient current changes but in the opposite direction. Analysis of the curve responses shows that the transient component of each curve is best described by a single, fast exponential term equation in case of Na addition to preparation unpoisonned with ouabain. In contrast, an equation including two exponential terms (a fast and a slow one) is required to fit the curve responses observed across ouabain treated epithelia or if Li is added outside. The transient responses were not significantly altered by substituying Cl for SO4(2)-anions. They were completely prevented by Amiloride (5-10(-5) M), increased by oxytocin (20 mU/ml) and markedly dependent upon the outer Na concentration. Interpreted in term of compartmental analysis, these observations suggest that a) the frog skin epithelium contains 2 separated but communicating compartments having different degrees of accessibility from outside; b) only that compartment filling at a fast rate (0.5 min) is involved in the transepithelial Na transport; c) the other one, filling at a rate of 4 to 7 min, is resplenished only under conditions where the basal pump system has a reduced activity. Tentative localization of these compartment is proposed."}
{"id": "PMID:1081679", "title": "Na and K movements across the membranes of frog skin epithelia associated with transient current changes.", "content": "The ionic composition of the current crossing each membrane of the frog skin epithelia during a) the positive and transient current responses elicited by sudden addition of Na ions to the outer Na free medium b) the negative and transient current responses observed when Na loaded preparations are suddenly exposed to outer Na free solution was determined using isotopic techniques. It is shown that Na ions carry the current across the outer membrane while K ions are mainly involved in the transfert of charges across the inner membrane. The amount of Na accumulated by the epithelial cells during the responses is correlated to the area under the transient part of the current responses. Determinations of 24Na uptake at different time of these transient positive responses show that the unidirectional Na influx across the outer membrane decreases as a function of time. It is suggested that the intracellular Na concentration might control the Na uptake mechanism across the outer membrane. During the negative responses, the Na efflux into the outer medium is highly correlated, either in time course or magnitude, to the current response. Both Na efflux and negative current are sensitive to amiloride, suggesting that the mechanism of Na uptake by the frog skin also is able to promote Na movement out of the epithelial cells.", "contents": "Na and K movements across the membranes of frog skin epithelia associated with transient current changes. The ionic composition of the current crossing each membrane of the frog skin epithelia during a) the positive and transient current responses elicited by sudden addition of Na ions to the outer Na free medium b) the negative and transient current responses observed when Na loaded preparations are suddenly exposed to outer Na free solution was determined using isotopic techniques. It is shown that Na ions carry the current across the outer membrane while K ions are mainly involved in the transfert of charges across the inner membrane. The amount of Na accumulated by the epithelial cells during the responses is correlated to the area under the transient part of the current responses. Determinations of 24Na uptake at different time of these transient positive responses show that the unidirectional Na influx across the outer membrane decreases as a function of time. It is suggested that the intracellular Na concentration might control the Na uptake mechanism across the outer membrane. During the negative responses, the Na efflux into the outer medium is highly correlated, either in time course or magnitude, to the current response. Both Na efflux and negative current are sensitive to amiloride, suggesting that the mechanism of Na uptake by the frog skin also is able to promote Na movement out of the epithelial cells."}
{"id": "PMID:1081680", "title": "The comparative effects of (Ca2+) and (Mg2+) on on tension generation in the fibers of skinned frog skeletal muscle and mechanically disrupted rat ventricular cardiac muscle.", "content": "The effects of intracellular Mg2+ on Ca2+-activated isometric tension generation in rat cardiac muscle fibers and frog skeletal muscle were compared. The membranous sarcolemmal barrier was removed from rat cardiac muscle fibers by mechanical disruption and from frog skeletal muscle by skinning. Tension was recorded in the fibers in bathing solutions of different Ca2+ concentrations and either 5 X 10(-5) M or 1 X 10(-3) M Mg2+ concentration (the same concentrations used in a previous study on single skinned frog skeletal muscle fibers [3]). The amount of Ca2+ required to activate the muscle increased with Mg2+ concentration for both rat ventricular muscle and frog skeletal muscle. These data indicate that intracellular Mg2+ concentration could strongly modulate Ca2+-activated tension in cardiac muscle and that very similar molecular mechanisms are responsible for Ca2+-activated tension in rat ventricular muscle and frog skeletal muscle. The possible sites of action of Mg2+ on Ca2+-activated tension are discussed.", "contents": "The comparative effects of (Ca2+) and (Mg2+) on on tension generation in the fibers of skinned frog skeletal muscle and mechanically disrupted rat ventricular cardiac muscle. The effects of intracellular Mg2+ on Ca2+-activated isometric tension generation in rat cardiac muscle fibers and frog skeletal muscle were compared. The membranous sarcolemmal barrier was removed from rat cardiac muscle fibers by mechanical disruption and from frog skeletal muscle by skinning. Tension was recorded in the fibers in bathing solutions of different Ca2+ concentrations and either 5 X 10(-5) M or 1 X 10(-3) M Mg2+ concentration (the same concentrations used in a previous study on single skinned frog skeletal muscle fibers [3]). The amount of Ca2+ required to activate the muscle increased with Mg2+ concentration for both rat ventricular muscle and frog skeletal muscle. These data indicate that intracellular Mg2+ concentration could strongly modulate Ca2+-activated tension in cardiac muscle and that very similar molecular mechanisms are responsible for Ca2+-activated tension in rat ventricular muscle and frog skeletal muscle. The possible sites of action of Mg2+ on Ca2+-activated tension are discussed."}
{"id": "PMID:1081681", "title": "Influence of the membrane stabilizer diphenylhydantoin on potassium and sodium movements in skeletal muscle.", "content": "The membrane stabilizer diphenylhydantoin (DPH) did not alter the net content of Na, K, Ca or Mg in frog sartorius muscle freshly incubated or actively transporting Na and K following Na-loading and K-depletion. Resting influx of K from normal Ringer was significantly reduced by DPH, and this inhibition occurred in the ouabain-insensitive K-uptake. Inhibition of K-influx by DPH was overcome when [K]0 was raised to 10 mM, and Rb-influx was not sensitive to the inhibitor in 2.5 mM Rb-Ringer. Efflux of tracer K was reduced by DPH in the presence and absence of ouabain. Exchange of muscle Na was not affected under conditions in which K-exchange was significantly reduced, but DPH appeared to cause increased net loss of Na from muscles washed in Na-free medium. The inhibition of DPH of resting K-exchange was not sensitive to wide variations in [Na]0 or in [Ca]0. The results suggest that the effect of DPH on frog skeletal muscle in normal ionic environment is to reduce the resting, passive component of K-exchange across the fibre membrane. This effect is discussed in relation to the membrane stabilizing actions of diphenylhydantoin.", "contents": "Influence of the membrane stabilizer diphenylhydantoin on potassium and sodium movements in skeletal muscle. The membrane stabilizer diphenylhydantoin (DPH) did not alter the net content of Na, K, Ca or Mg in frog sartorius muscle freshly incubated or actively transporting Na and K following Na-loading and K-depletion. Resting influx of K from normal Ringer was significantly reduced by DPH, and this inhibition occurred in the ouabain-insensitive K-uptake. Inhibition of K-influx by DPH was overcome when [K]0 was raised to 10 mM, and Rb-influx was not sensitive to the inhibitor in 2.5 mM Rb-Ringer. Efflux of tracer K was reduced by DPH in the presence and absence of ouabain. Exchange of muscle Na was not affected under conditions in which K-exchange was significantly reduced, but DPH appeared to cause increased net loss of Na from muscles washed in Na-free medium. The inhibition of DPH of resting K-exchange was not sensitive to wide variations in [Na]0 or in [Ca]0. The results suggest that the effect of DPH on frog skeletal muscle in normal ionic environment is to reduce the resting, passive component of K-exchange across the fibre membrane. This effect is discussed in relation to the membrane stabilizing actions of diphenylhydantoin."}
{"id": "PMID:1081682", "title": "[Treatment of hemophilia with immunologic inhibitor of factor VIII by using \"activated\" coagulant fractions].", "content": "Bleeding episodes in hemophiliacs with inhibitor to factor VIII are often dramatic situations. High level inhibitors do not respond to massive amount of factor VIII and are often refractory to immunosuppressive drugs such as cyclophosphamide. In this situation it has been proposed to use \"activated prothrombin complex\" to control bleeding in hemophilic patients with inhibitors. A plasmatic fraction \"Auto IX\" has been used successfully for the treatment of an intestinal hemorrhage in a patient with an inhibitor to F. VIII. Possible mechanisms of action as well as potential thrombotic danger of such concentrate are discussed.", "contents": "[Treatment of hemophilia with immunologic inhibitor of factor VIII by using \"activated\" coagulant fractions]. Bleeding episodes in hemophiliacs with inhibitor to factor VIII are often dramatic situations. High level inhibitors do not respond to massive amount of factor VIII and are often refractory to immunosuppressive drugs such as cyclophosphamide. In this situation it has been proposed to use \"activated prothrombin complex\" to control bleeding in hemophilic patients with inhibitors. A plasmatic fraction \"Auto IX\" has been used successfully for the treatment of an intestinal hemorrhage in a patient with an inhibitor to F. VIII. Possible mechanisms of action as well as potential thrombotic danger of such concentrate are discussed."}
{"id": "PMID:1081686", "title": "[Is triacetyl oleandomycin-ergotamine tartrate combination dangerous?].", "content": "The authors report a case of ischaemia of all four limbs in an adolescent following the simultaneous ingestion of triacetyl oleandomycin and ergotamine tartrate in low dosage. Two cases involving this same association of medications have appeared in the literature and attention is therefore drawn to the possible danger of use of these two drugs together.", "contents": "[Is triacetyl oleandomycin-ergotamine tartrate combination dangerous?]. The authors report a case of ischaemia of all four limbs in an adolescent following the simultaneous ingestion of triacetyl oleandomycin and ergotamine tartrate in low dosage. Two cases involving this same association of medications have appeared in the literature and attention is therefore drawn to the possible danger of use of these two drugs together."}
{"id": "PMID:1081692", "title": "Components of O2-uptake by excised frog nerve dependent upon externally supplied sodium ions.", "content": "The steady rate of oxygen uptake of excised frog nerves equilibrated in a solution having a very low concentration of sodium ions increases to a new high steady rate when equilibrated with a solution containing a high concentration of this ion. The increase is suppressed by ouabain, indicating participation of the sodium pump. Part of this sodium-activated increase in oxygen uptake is inhibited by tetrodotoxin, indicating that passive influx of sodium ions into axons is part of the total process. Thus, two pathways for passive sodium influx into axons are suggested by these experiments. Procedures known to increase the passive permeability of axons for sodium ions also increase this sodium-activated oxygen uptake. A mechanism is proposed to explain that part of the sodium-activated oxygen uptake that is inhibited by tetrodotoxin.", "contents": "Components of O2-uptake by excised frog nerve dependent upon externally supplied sodium ions. The steady rate of oxygen uptake of excised frog nerves equilibrated in a solution having a very low concentration of sodium ions increases to a new high steady rate when equilibrated with a solution containing a high concentration of this ion. The increase is suppressed by ouabain, indicating participation of the sodium pump. Part of this sodium-activated increase in oxygen uptake is inhibited by tetrodotoxin, indicating that passive influx of sodium ions into axons is part of the total process. Thus, two pathways for passive sodium influx into axons are suggested by these experiments. Procedures known to increase the passive permeability of axons for sodium ions also increase this sodium-activated oxygen uptake. A mechanism is proposed to explain that part of the sodium-activated oxygen uptake that is inhibited by tetrodotoxin."}
{"id": "PMID:1081693", "title": "New approach to antifolate treatment of certain cancers as demonstrated in tissue culture.", "content": "The selective toxicity of antifolates for a variety of cancers can be improved, as illustrated by the combined administration of N5-methyltetrahydrofolate and methotrexate in tissue culture. When a variety of neoplastic cell types characterized by a deficiency of vitamin B12-dependent N5-methyltetrahydrofolate methyltransferase (5-methyltetrahydropteroyl-L-glutamate:L-homocysteine S-methyltransferase, EC 2.1.1.13) and normal adult cells are grown in media containing methotrexate and either N5-methyltetrahydrofolate or N5-formyltetrahydrofolate, not only is the selective toxicity of methotrexate demonstrated, but the advantage of using N5-methyltetrahydrofolate in place of N5-formyltetrahydrofolate is also revealed. The implications and applications of this particular combination in the treatment of human cancer are discussed.", "contents": "New approach to antifolate treatment of certain cancers as demonstrated in tissue culture. The selective toxicity of antifolates for a variety of cancers can be improved, as illustrated by the combined administration of N5-methyltetrahydrofolate and methotrexate in tissue culture. When a variety of neoplastic cell types characterized by a deficiency of vitamin B12-dependent N5-methyltetrahydrofolate methyltransferase (5-methyltetrahydropteroyl-L-glutamate:L-homocysteine S-methyltransferase, EC 2.1.1.13) and normal adult cells are grown in media containing methotrexate and either N5-methyltetrahydrofolate or N5-formyltetrahydrofolate, not only is the selective toxicity of methotrexate demonstrated, but the advantage of using N5-methyltetrahydrofolate in place of N5-formyltetrahydrofolate is also revealed. The implications and applications of this particular combination in the treatment of human cancer are discussed."}
{"id": "PMID:1081694", "title": "Membrane particles on fracture faces of frozen myelin.", "content": "Freeze-fracture electron microscopy reveals constant and widespread presence of membrane particles on the fracture faces of frozen myelin. In unfixed myelin frozen shortly after dissection the distribution of the particles is uniform. In glutaraldehyde-fixed and/or glycerol-impregnated myelin the particles frequently occupy a network interspersed with circumscribed particle-free areas of variable dimension. In these membranes the network of particles is propagated radially across many lamellae. Particle-rich areas are closely apposed and contrast with frequent delamination of adjacent particle-free regions. We propose that, as in other plasma membranes, the particles of myelin represent protein-containing structures intercalated across the hydrophobic matrix of a membrane with bilayer organization. Our results indicate that these structures contain sites which mutually interact at the surface of apposed membranes and may be important in maintaining the organizational integrity of myelin.", "contents": "Membrane particles on fracture faces of frozen myelin. Freeze-fracture electron microscopy reveals constant and widespread presence of membrane particles on the fracture faces of frozen myelin. In unfixed myelin frozen shortly after dissection the distribution of the particles is uniform. In glutaraldehyde-fixed and/or glycerol-impregnated myelin the particles frequently occupy a network interspersed with circumscribed particle-free areas of variable dimension. In these membranes the network of particles is propagated radially across many lamellae. Particle-rich areas are closely apposed and contrast with frequent delamination of adjacent particle-free regions. We propose that, as in other plasma membranes, the particles of myelin represent protein-containing structures intercalated across the hydrophobic matrix of a membrane with bilayer organization. Our results indicate that these structures contain sites which mutually interact at the surface of apposed membranes and may be important in maintaining the organizational integrity of myelin."}
{"id": "PMID:1081699", "title": "Near-fatal hemorrhage following gastrografin studies.", "content": "The use of upper gastrointestinal studies followed by gastric drainage and observation is recommended for the evaluation and treatment of stomal dysfunction due to edema or gastric atony. Gastrografin though, may be precipitated out and can cause gastrointestinal bleeding if left in the gastric remenant. This agent should be used with proper precautions in the evaluation of postoperative gastric retention.", "contents": "Near-fatal hemorrhage following gastrografin studies. The use of upper gastrointestinal studies followed by gastric drainage and observation is recommended for the evaluation and treatment of stomal dysfunction due to edema or gastric atony. Gastrografin though, may be precipitated out and can cause gastrointestinal bleeding if left in the gastric remenant. This agent should be used with proper precautions in the evaluation of postoperative gastric retention."}
{"id": "PMID:1081700", "title": "Participation of the external and internal carotid arteries in the blood supply of acoustic neurinomas.", "content": "Six cases of acoustic neurinoma are presented in which the tumor is supplied by branches of the external carotid artery, with or without supply from the internal carotid artery. A new angiographic protocol for the investigation of masses in the cerebellopontine angle is suggested. According to early results of the CT scan, acoustic neurinomas always appear to be hypervascular following the injection of contrast media.", "contents": "Participation of the external and internal carotid arteries in the blood supply of acoustic neurinomas. Six cases of acoustic neurinoma are presented in which the tumor is supplied by branches of the external carotid artery, with or without supply from the internal carotid artery. A new angiographic protocol for the investigation of masses in the cerebellopontine angle is suggested. According to early results of the CT scan, acoustic neurinomas always appear to be hypervascular following the injection of contrast media."}
{"id": "PMID:1081725", "title": "Mitogens of B and T cells from peripheral blood in rheumatoid arthritis.", "content": "Patients with rheumatoid arthritis (RA), 36 cases, and normal subjects, 49 cases were studied by lymphocyte cultures stimulated by phytohemagglutinin (PHA), Concanavalin A (Con A), pokeweed mitogen (PWM) and Con A convalently bound to Sepharose 4 B (Con A-S). The comparisons between the two groups showed a significant difference between the RA lymphocytes and the control lymphocytes stimulated by PHA and Con A. However, no statistical difference was found between the two lymphocyte populations stimulated by PWM and Con A-S. In order to determine the lymphocyte population stimulated by each mitogen, separation of B and T cells from peripheral blood was performed according to the ability for the T cell population to bind the sheep red blood cells (rosette-forming cells). The T cell-rich population was only stimulated by PHA, Con A and PWM. Although the T cell-depleted population showed no response to these mitogens, a response to Con A-S was elicited.", "contents": "Mitogens of B and T cells from peripheral blood in rheumatoid arthritis. Patients with rheumatoid arthritis (RA), 36 cases, and normal subjects, 49 cases were studied by lymphocyte cultures stimulated by phytohemagglutinin (PHA), Concanavalin A (Con A), pokeweed mitogen (PWM) and Con A convalently bound to Sepharose 4 B (Con A-S). The comparisons between the two groups showed a significant difference between the RA lymphocytes and the control lymphocytes stimulated by PHA and Con A. However, no statistical difference was found between the two lymphocyte populations stimulated by PWM and Con A-S. In order to determine the lymphocyte population stimulated by each mitogen, separation of B and T cells from peripheral blood was performed according to the ability for the T cell population to bind the sheep red blood cells (rosette-forming cells). The T cell-rich population was only stimulated by PHA, Con A and PWM. Although the T cell-depleted population showed no response to these mitogens, a response to Con A-S was elicited."}
{"id": "PMID:1081726", "title": "Studies on antilymphocyte antibodies in patients with rheumatoid arthritis and systemic lupus erythematosus.", "content": "Anti-lymphocyte antibodies have been demonstrated in patients with SLE and RA by immunofluorescence. They differ from those arising through iso-immunization by being cold reactive and chiefly of the IgM class. Separate anti specificities were shown for determinants on either T or B cells.", "contents": "Studies on antilymphocyte antibodies in patients with rheumatoid arthritis and systemic lupus erythematosus. Anti-lymphocyte antibodies have been demonstrated in patients with SLE and RA by immunofluorescence. They differ from those arising through iso-immunization by being cold reactive and chiefly of the IgM class. Separate anti specificities were shown for determinants on either T or B cells."}
{"id": "PMID:1081729", "title": "Evaluation of serum thymic hormone and of circulating T cells in rheumatoid arthritis and in systemic lupus erythematosus.", "content": "The presence of a serum factor secreted by the thymus has been demonstrated in the serum of mice and man using a rosette inhibition assay. This factor is a peptide with a molecular weight close to 1,000. Its level is age dependent, being stable until the age of 6 months in mice and until 20 years in man. New Zealand Black mice show a premature cessation of thymic hormone secretion preceeding the onset of other T cell abnormalities and auto-immunity. Before any treatment the serum thymic hormone level is abnormally low in most cases of SLE even in patients under 25, an age where control subjects still show high hormone levels. Conversely, normal or high levels are found in RA, PARTICularly in patients over 40 who showed in 60% of cases a hormone level significantly higher than normal controls of the same age. In keeping with this data T cells evaluated by SRBC spontaneous rosette formation show low figures in some patients with active SLE and normal or high values in most cases of RA.", "contents": "Evaluation of serum thymic hormone and of circulating T cells in rheumatoid arthritis and in systemic lupus erythematosus. The presence of a serum factor secreted by the thymus has been demonstrated in the serum of mice and man using a rosette inhibition assay. This factor is a peptide with a molecular weight close to 1,000. Its level is age dependent, being stable until the age of 6 months in mice and until 20 years in man. New Zealand Black mice show a premature cessation of thymic hormone secretion preceeding the onset of other T cell abnormalities and auto-immunity. Before any treatment the serum thymic hormone level is abnormally low in most cases of SLE even in patients under 25, an age where control subjects still show high hormone levels. Conversely, normal or high levels are found in RA, PARTICularly in patients over 40 who showed in 60% of cases a hormone level significantly higher than normal controls of the same age. In keeping with this data T cells evaluated by SRBC spontaneous rosette formation show low figures in some patients with active SLE and normal or high values in most cases of RA."}
{"id": "PMID:1081730", "title": "Evidence for complement activation by the alternate pathway in the arthritis of hypogammaglobulinemic patients.", "content": "The chronic, rheumatoid synovitis in four patients (two children and two adults) with immunodeficiency was studied by means of immunopathological examination of synovial tissues and fluids. Their immunodeficiencies were extensively studied, with various tests for humoral and cell-mediated immunity. The two children were boys with Bruton's disease. One adult, female, had common variable immunodeficiency. One adult male had severe hypogammaglobulinemia. All patients had isolated deposits of complement component C3 in their synovial membranes, usually without traces of immunoglobulins. Depressed levels of complement component C3 were found in the joint fluid, in contrast to high levels in the corresponding serum in one patient. Components of the alternate pathway, properdin and C3A, were found in the tissues, but not components of the classic pathway, C1q and C4. The findings suggest that chronic, rheumatoid arthritis in hypogammaglobulinemic patients may be related to activation of C3 by the alternate pathway.", "contents": "Evidence for complement activation by the alternate pathway in the arthritis of hypogammaglobulinemic patients. The chronic, rheumatoid synovitis in four patients (two children and two adults) with immunodeficiency was studied by means of immunopathological examination of synovial tissues and fluids. Their immunodeficiencies were extensively studied, with various tests for humoral and cell-mediated immunity. The two children were boys with Bruton's disease. One adult, female, had common variable immunodeficiency. One adult male had severe hypogammaglobulinemia. All patients had isolated deposits of complement component C3 in their synovial membranes, usually without traces of immunoglobulins. Depressed levels of complement component C3 were found in the joint fluid, in contrast to high levels in the corresponding serum in one patient. Components of the alternate pathway, properdin and C3A, were found in the tissues, but not components of the classic pathway, C1q and C4. The findings suggest that chronic, rheumatoid arthritis in hypogammaglobulinemic patients may be related to activation of C3 by the alternate pathway."}
{"id": "PMID:1081732", "title": "Study of the effects of antigammaglobulin factors on lymphocytes from patients with rheumatoid arthritis.", "content": "Previous work suggests that peripheral blood lymphocytes from patients with rheumatoid arthritis have altered reactivity. The purpose of this study has been to observe the behavior of cultures of lymphocytes from patients with rheumatoid arthritis when stimulated with autologous 19S rheumatoid factor and with rabbit serum against autologous IgG fraction. The peripheral blood lymphocytes (PBL) from 15 patients with classical rheumatoid arthritis were obtained by passage of the leukocyte-rich plasma through a polyamide fiber column. The purified lymphocytes were cultured with rheumatoid factor obtained from the same donor through Sephadex G-200 chromatography. In another set of experiments rheumatoid lymphocytes were stimulated with rabbit serum containing antibodies against the IgG fraction of the same patient obtained through DEAE chromatography. After five days the lymphocytes were examined for large cells plus cells in mitosis. A positive response was defined as an increase of 5% or more in the experimental tubes when compared with the cells cultured alone. Ten healthy normal subjects were used also as lymphocyte and serum donors. These lymphocytes were cultured alone and with rabbit serum, anti-autologous IgG. 80% of rheumatoid lymphocytes responded with an average of 37% of enlarging and dividing cells when stimulated by autologous RF. The same pannel of lymphocytes responded only with an average of 14% stimulation when cultured with rabbit serum anti-autologous IgG. The response to the 7S antigammaglobulin factor from rabbit in 7 normal PBL was similar to that of rheumatoid lymphocytes. There was some relation between activity of the RA and lymphocyte response to RF and rabbit antiserum. These results could explain the lymphoid infiltration and hyperplasia of patients with RA and, on the other hand, would support the idea that entrapment of RF by leukocytes ('RA cell') would be a protective mechanism.", "contents": "Study of the effects of antigammaglobulin factors on lymphocytes from patients with rheumatoid arthritis. Previous work suggests that peripheral blood lymphocytes from patients with rheumatoid arthritis have altered reactivity. The purpose of this study has been to observe the behavior of cultures of lymphocytes from patients with rheumatoid arthritis when stimulated with autologous 19S rheumatoid factor and with rabbit serum against autologous IgG fraction. The peripheral blood lymphocytes (PBL) from 15 patients with classical rheumatoid arthritis were obtained by passage of the leukocyte-rich plasma through a polyamide fiber column. The purified lymphocytes were cultured with rheumatoid factor obtained from the same donor through Sephadex G-200 chromatography. In another set of experiments rheumatoid lymphocytes were stimulated with rabbit serum containing antibodies against the IgG fraction of the same patient obtained through DEAE chromatography. After five days the lymphocytes were examined for large cells plus cells in mitosis. A positive response was defined as an increase of 5% or more in the experimental tubes when compared with the cells cultured alone. Ten healthy normal subjects were used also as lymphocyte and serum donors. These lymphocytes were cultured alone and with rabbit serum, anti-autologous IgG. 80% of rheumatoid lymphocytes responded with an average of 37% of enlarging and dividing cells when stimulated by autologous RF. The same pannel of lymphocytes responded only with an average of 14% stimulation when cultured with rabbit serum anti-autologous IgG. The response to the 7S antigammaglobulin factor from rabbit in 7 normal PBL was similar to that of rheumatoid lymphocytes. There was some relation between activity of the RA and lymphocyte response to RF and rabbit antiserum. These results could explain the lymphoid infiltration and hyperplasia of patients with RA and, on the other hand, would support the idea that entrapment of RF by leukocytes ('RA cell') would be a protective mechanism."}
{"id": "PMID:1081734", "title": "The failure of allogeneic cells to maintain an immune response in nude mice.", "content": "Nude mice that have been injected with allogeneic thymus cells and sheep erythrocytes respond for a short time and are then unable to respond to subsequent injections of sheep erythrocytes, even if given with allogeneic thymus cells from the same or a different strain. However, they can respond to a different antigen or to the original antigen if given with congenic thymus cells. B cells from such animals are able to respond if transferred together with congenic thymocytes to irradiated hosts. It is suggested that allogeneic T cells are able to cooperate with B cells to initiate an immune response but that a further step is necessary to maintain the response, and for this congenic T cells are necessary.", "contents": "The failure of allogeneic cells to maintain an immune response in nude mice. Nude mice that have been injected with allogeneic thymus cells and sheep erythrocytes respond for a short time and are then unable to respond to subsequent injections of sheep erythrocytes, even if given with allogeneic thymus cells from the same or a different strain. However, they can respond to a different antigen or to the original antigen if given with congenic thymus cells. B cells from such animals are able to respond if transferred together with congenic thymocytes to irradiated hosts. It is suggested that allogeneic T cells are able to cooperate with B cells to initiate an immune response but that a further step is necessary to maintain the response, and for this congenic T cells are necessary."}
{"id": "PMID:1081735", "title": "Macrophages suppress direct B-cell activation by lipopolysaccharide.", "content": "The influence of macrophages on polyclonal B-cell responses to lipopolysaccharide (LPS) and on a primary, specific immune response to a hapten-LPS conjugate was studied in mouse spleen and lymph node cells in serum-free and serum-supplemented cultures. Macrophages were found not to be necessary, and B cells were directly activated by LPS. In serum-free cultures of macrophage-depleted spleen cells, (a) proliferation and antibody secretion occurred at normal levels or were enhanced when compared with normal spleen cells, (b) the responsiveness of limiting cell numbers to LPS was better than in normal spleen cell cultures, (c) LPS did not increase the number or activate residual macrophages, (d) the primary specific response to a hapten-LPS conjugate developed normally, (e) peripheral lymph node cells, which are known to contain a very low concentration of macrophages, from normal or congenitally athymic (nude) mice mounted excellent proliferative responses to LPS. Furthermore, in cultures supplemented with fetal bovine serum, depletion of adherent cells resulted in enhancement of LPS-induced B-cell responses. Addition of peritoneal macrophages to adherent cell-depleted spleen cells produced suppression at all concentrations of both mitogen and adherent cells. Suppressive activity could also be demonstrated in supernatants from adherent cell cultures stimulated by LPS.", "contents": "Macrophages suppress direct B-cell activation by lipopolysaccharide. The influence of macrophages on polyclonal B-cell responses to lipopolysaccharide (LPS) and on a primary, specific immune response to a hapten-LPS conjugate was studied in mouse spleen and lymph node cells in serum-free and serum-supplemented cultures. Macrophages were found not to be necessary, and B cells were directly activated by LPS. In serum-free cultures of macrophage-depleted spleen cells, (a) proliferation and antibody secretion occurred at normal levels or were enhanced when compared with normal spleen cells, (b) the responsiveness of limiting cell numbers to LPS was better than in normal spleen cell cultures, (c) LPS did not increase the number or activate residual macrophages, (d) the primary specific response to a hapten-LPS conjugate developed normally, (e) peripheral lymph node cells, which are known to contain a very low concentration of macrophages, from normal or congenitally athymic (nude) mice mounted excellent proliferative responses to LPS. Furthermore, in cultures supplemented with fetal bovine serum, depletion of adherent cells resulted in enhancement of LPS-induced B-cell responses. Addition of peritoneal macrophages to adherent cell-depleted spleen cells produced suppression at all concentrations of both mitogen and adherent cells. Suppressive activity could also be demonstrated in supernatants from adherent cell cultures stimulated by LPS."}
{"id": "PMID:1081736", "title": "Differentiation of T cells in nude mice.", "content": "Cells bearing the T-cell differentiation alloantigens TL and Thy-1 were enumerated in preparations of spleen and lymph node cells of nu/nu mice. Healthy nu/nu mice had few or no demonstrable TL+ or Thy-1+ cells whereas mice with viral hepatitis, including some born of nu/nu X nu/nu matings, had many. Healthy nu/nu mice were treated daily with the thymic hormone thymopoietin or with ubiquitin, polypeptides that induce the differentiation of TL+Thy-1\" cells from TL-Thy-1- precursors in vitro. After 14 days, 20 to 40 percent of their spleen cells were of the TL+Thy-1+ phenotype typical of thymocytes and 10 to 25 percent of their lymph node cells were TL-Thy-1+, typical of later T-cell differentiation. Similar frequencies of such cells were found in untreated nu/nu mice suffering from severe viral hepatitis. These data conform to the current view that prothymocytes are preprogrammed cells whose maturation to thymocytes, normally induced in the thymus by thymopoietin, can be triggered by other agents under abnormal circumstances. Tests of T-cell function were not included in this study.", "contents": "Differentiation of T cells in nude mice. Cells bearing the T-cell differentiation alloantigens TL and Thy-1 were enumerated in preparations of spleen and lymph node cells of nu/nu mice. Healthy nu/nu mice had few or no demonstrable TL+ or Thy-1+ cells whereas mice with viral hepatitis, including some born of nu/nu X nu/nu matings, had many. Healthy nu/nu mice were treated daily with the thymic hormone thymopoietin or with ubiquitin, polypeptides that induce the differentiation of TL+Thy-1\" cells from TL-Thy-1- precursors in vitro. After 14 days, 20 to 40 percent of their spleen cells were of the TL+Thy-1+ phenotype typical of thymocytes and 10 to 25 percent of their lymph node cells were TL-Thy-1+, typical of later T-cell differentiation. Similar frequencies of such cells were found in untreated nu/nu mice suffering from severe viral hepatitis. These data conform to the current view that prothymocytes are preprogrammed cells whose maturation to thymocytes, normally induced in the thymus by thymopoietin, can be triggered by other agents under abnormal circumstances. Tests of T-cell function were not included in this study."}
{"id": "PMID:1081737", "title": "Coronary artery bypass for acute myocardial infarction.", "content": "Coronary artery bypass has been done on 20 patients for acute mmyocardial infarction associated with shock, arrhythmia, and/or cardiac arrest. Twelve had had catheterization before the time of infarction. There were 3 hospital deaths, 2 late deaths, and 15 survivors. Survivors are angina-free and active 4 to 40 months postoperatively. Five patients have been recatheterized since operation, and in 4 all grafts are patent and functioning. Ventricular function was not improved in one patient, has remained the same in one, and improved in 3 patients. Although the time interval from infarction to revascularization is important, success is just as dependent on the patient's existing coronary collateral circulation. Lesions of the left main coronary artery were associated with the highest mortality. The rationale for operation in patients with acute myocardial infarction is to revascularize the marginal myocardium adjacent to the infarct and other underperfused areas when indicated. Chance for survival may thereby be increased by improvement of cardiac pumping or reversal of cardiac arrest or arrhythmia.", "contents": "Coronary artery bypass for acute myocardial infarction. Coronary artery bypass has been done on 20 patients for acute mmyocardial infarction associated with shock, arrhythmia, and/or cardiac arrest. Twelve had had catheterization before the time of infarction. There were 3 hospital deaths, 2 late deaths, and 15 survivors. Survivors are angina-free and active 4 to 40 months postoperatively. Five patients have been recatheterized since operation, and in 4 all grafts are patent and functioning. Ventricular function was not improved in one patient, has remained the same in one, and improved in 3 patients. Although the time interval from infarction to revascularization is important, success is just as dependent on the patient's existing coronary collateral circulation. Lesions of the left main coronary artery were associated with the highest mortality. The rationale for operation in patients with acute myocardial infarction is to revascularize the marginal myocardium adjacent to the infarct and other underperfused areas when indicated. Chance for survival may thereby be increased by improvement of cardiac pumping or reversal of cardiac arrest or arrhythmia."}
{"id": "PMID:1081738", "title": "Infections after retinal detachment surgery.", "content": "The signs and symptoms of infection after retinal detachment surgery may be subtle and frequently are overlooked during early stages. A retrospective analysis of 3,334 consecutive procedures for retinal detachment revealed 37 cases (1.1%) with postoperative infection. In 13 of these a scleral abscess and a form of endophthalmitis developed, whereas 24 had no evidence of vitreous involvement and were classified as the granuloma type. Only 43% of infected patients had positive cultures. Analysis of changes in infection rate involved comparison with changes over the years in suture material, implant material, performance of scleral undermining, drainage of subretinal fluid, number of previous surgical procedures, age of patient, use of diathermy or cryotherapy, and type of prophylactic antibiotic therapy. During the 13 years studied the rate of infection decreased from 2.2% to 0.7%. Treatment requires surgical removal of all foreign material before the inflammatory signs can be cleared. Concurrent appropriate antibiotic therapy also is recommended. With this form of treatment, all but one patient showed a satisfactory response, although 4 patients still have mild conjunctival congestion.", "contents": "Infections after retinal detachment surgery. The signs and symptoms of infection after retinal detachment surgery may be subtle and frequently are overlooked during early stages. A retrospective analysis of 3,334 consecutive procedures for retinal detachment revealed 37 cases (1.1%) with postoperative infection. In 13 of these a scleral abscess and a form of endophthalmitis developed, whereas 24 had no evidence of vitreous involvement and were classified as the granuloma type. Only 43% of infected patients had positive cultures. Analysis of changes in infection rate involved comparison with changes over the years in suture material, implant material, performance of scleral undermining, drainage of subretinal fluid, number of previous surgical procedures, age of patient, use of diathermy or cryotherapy, and type of prophylactic antibiotic therapy. During the 13 years studied the rate of infection decreased from 2.2% to 0.7%. Treatment requires surgical removal of all foreign material before the inflammatory signs can be cleared. Concurrent appropriate antibiotic therapy also is recommended. With this form of treatment, all but one patient showed a satisfactory response, although 4 patients still have mild conjunctival congestion."}
{"id": "PMID:1081742", "title": "Splenic artery ligation in selected patients with hepatic cirrhosis and in Sprague-Dawley rats.", "content": "The effects of splenic artery ligation were studied in Sprague-Dawley rats and in eight selected symptomatic patients with hepatic cirrhosis. In rats, this maneuver induced splenic infarction, reduced functional splenic mass, transiently raised platelet and reticulocyte counts and was without local complications. In seven selected patients with cirrhosis and prominent splenomegaly, the splenic artery was markedly enlarged, splenic arterial flow was greatly increased and splenic artery ligation partially lowered portal pressure. In three patients with varying cytopenias secondary to hypersplenism splenic artery ligation uniformly improved peripheral blood elements, although varying degrees of hypersplenism later recurred necessitating splenectomy in one. In five other patients, splenic artery ligation in conjunction with coronary vein ligation in four was performed for bleeding esophageal varices. Two patients later required portacaval shunting, and one other in whom operation was undertaken in desperation died of hepatic failure. Celiac-mesenteric arterioportography, operative portography, hemodynamic measurements and examination of peripheral blood elements in these eight patients suggests that splenic artery ligation in conjunction, where appropriate, with coronary vein ligation has several potentially beneficial effects. Hypersplenism may be sufficiently controlled to alleviate clinical symptoms. Arterial inflow into the portal system is reduced tending to lower portal pressure. Transheptic portal flow from the mesenteric bed is preserved. Venous anastomotic channels still functioning around the splenic pedicle and no longer draining a hyperdynamic splenic circuit may be converted into an escape route for mesenteric venous blood entering the portal system under high pressure. Nonetheless, each of these effects and their interrelationships require further study before this operation assumes a larger role in the treatment of complications of portal hypertension.", "contents": "Splenic artery ligation in selected patients with hepatic cirrhosis and in Sprague-Dawley rats. The effects of splenic artery ligation were studied in Sprague-Dawley rats and in eight selected symptomatic patients with hepatic cirrhosis. In rats, this maneuver induced splenic infarction, reduced functional splenic mass, transiently raised platelet and reticulocyte counts and was without local complications. In seven selected patients with cirrhosis and prominent splenomegaly, the splenic artery was markedly enlarged, splenic arterial flow was greatly increased and splenic artery ligation partially lowered portal pressure. In three patients with varying cytopenias secondary to hypersplenism splenic artery ligation uniformly improved peripheral blood elements, although varying degrees of hypersplenism later recurred necessitating splenectomy in one. In five other patients, splenic artery ligation in conjunction with coronary vein ligation in four was performed for bleeding esophageal varices. Two patients later required portacaval shunting, and one other in whom operation was undertaken in desperation died of hepatic failure. Celiac-mesenteric arterioportography, operative portography, hemodynamic measurements and examination of peripheral blood elements in these eight patients suggests that splenic artery ligation in conjunction, where appropriate, with coronary vein ligation has several potentially beneficial effects. Hypersplenism may be sufficiently controlled to alleviate clinical symptoms. Arterial inflow into the portal system is reduced tending to lower portal pressure. Transheptic portal flow from the mesenteric bed is preserved. Venous anastomotic channels still functioning around the splenic pedicle and no longer draining a hyperdynamic splenic circuit may be converted into an escape route for mesenteric venous blood entering the portal system under high pressure. Nonetheless, each of these effects and their interrelationships require further study before this operation assumes a larger role in the treatment of complications of portal hypertension."}
{"id": "PMID:1081746", "title": "Ristocetin in the diagnosis of von willebrand's disease: a comparison of rate and percent of aggregation with levels of the plasma factor(s) necessary for ristocetin aggregation.", "content": "Percent aggregation and the aggregation rate of platelet rich plasma (PRP) in response to ristocetin (1.75 mg/ml) were measured in 20 normals and 16 patients with von Willebrand's disease (v Wd), with and without the addition of acetylsalicylic acid (ASA). Percent aggregation did not clearly distinguish between normals and patients with vWd. Aggregation rate was normal in only 2 of 16 patients, and after incubation of PRP with ASA 1 of these 2 remained normal. The corrective effect of dilutions of platelet poor plasma (PPP) on the ristocetin response of washed platelets (von Willebrand's factor, vWf) was measured in 21 normals and 12 patients with vWd. All patients with vWd had abnormal levels. There was a significant correlation between aggregation rate and vWf in patients with vWd but not in normals. Both tests appear to measure closely related defects, and the aggregation rate is as specific as the vWf level for the diagnosis of clinically affected patients.", "contents": "Ristocetin in the diagnosis of von willebrand's disease: a comparison of rate and percent of aggregation with levels of the plasma factor(s) necessary for ristocetin aggregation. Percent aggregation and the aggregation rate of platelet rich plasma (PRP) in response to ristocetin (1.75 mg/ml) were measured in 20 normals and 16 patients with von Willebrand's disease (v Wd), with and without the addition of acetylsalicylic acid (ASA). Percent aggregation did not clearly distinguish between normals and patients with vWd. Aggregation rate was normal in only 2 of 16 patients, and after incubation of PRP with ASA 1 of these 2 remained normal. The corrective effect of dilutions of platelet poor plasma (PPP) on the ristocetin response of washed platelets (von Willebrand's factor, vWf) was measured in 21 normals and 12 patients with vWd. All patients with vWd had abnormal levels. There was a significant correlation between aggregation rate and vWf in patients with vWd but not in normals. Both tests appear to measure closely related defects, and the aggregation rate is as specific as the vWf level for the diagnosis of clinically affected patients."}
{"id": "PMID:1081744", "title": "Keratomalacia.", "content": "Xerophthalmia and keratomalacia are public health problems of great magnitude which are usually associated with multiple vitamin and protein deficiencies. The authors report the case of a 27-year-old commune member who subjected herself to a bizarre protein and vitamin deficient diet for many months. This ultimately produced nyctalopia, xerophthalmia and keratomalacia with bilateral corneal perforation. Despite therapy, she remained comatose and expired shortly after admission. Ocular pathological changes included bilateral corneal melting with prolapse of intraocular contents, conjunctival epidermidalization, goblet cell atrophy and thinning of the outer nuclear layer of the retina. It is noted that ocular findings in pure avitaminosis A produced experimentalyy include epithelial atrophy followed by keratinization.", "contents": "Keratomalacia. Xerophthalmia and keratomalacia are public health problems of great magnitude which are usually associated with multiple vitamin and protein deficiencies. The authors report the case of a 27-year-old commune member who subjected herself to a bizarre protein and vitamin deficient diet for many months. This ultimately produced nyctalopia, xerophthalmia and keratomalacia with bilateral corneal perforation. Despite therapy, she remained comatose and expired shortly after admission. Ocular pathological changes included bilateral corneal melting with prolapse of intraocular contents, conjunctival epidermidalization, goblet cell atrophy and thinning of the outer nuclear layer of the retina. It is noted that ocular findings in pure avitaminosis A produced experimentalyy include epithelial atrophy followed by keratinization."}
{"id": "PMID:1081762", "title": "[Banding for pulmonary artery and subsequent repair in large ventricular septal defect (author's transl)].", "content": "Banding of the pulmonary artery was performed in 233 children with large, non restrictive ventricular septal defects. The group of isolated large VSDs consisted of 95 patients, of whom 8 died (8,4 per cent). 32 patients were subjected to open-heart operation for closure of the VSD and debanding. In this \"two-stage group\" the mortality rate was 6,3 per cent. At the second operation the pulmonary artery always should be enlarged with a pericardial patch. Our recordings up to 6 1/2 years after correction show no gradients left in cases surgically treated in this manner. The time-interval between effective banding and debanding procedure may not be limited strictly; the subsequent operation however should be done within two or three years.", "contents": "[Banding for pulmonary artery and subsequent repair in large ventricular septal defect (author's transl)]. Banding of the pulmonary artery was performed in 233 children with large, non restrictive ventricular septal defects. The group of isolated large VSDs consisted of 95 patients, of whom 8 died (8,4 per cent). 32 patients were subjected to open-heart operation for closure of the VSD and debanding. In this \"two-stage group\" the mortality rate was 6,3 per cent. At the second operation the pulmonary artery always should be enlarged with a pericardial patch. Our recordings up to 6 1/2 years after correction show no gradients left in cases surgically treated in this manner. The time-interval between effective banding and debanding procedure may not be limited strictly; the subsequent operation however should be done within two or three years."}
{"id": "PMID:1081763", "title": "[Ventricular septal defect associated with fibrous subvalvular aortic stenosis: problems in the diagnosis and in the surgical management (author's transl)].", "content": "Only 9 of more than 900 operated patients with ventricular septal defects suffered from this rare combination. The problems of the diagnosis are discussed. Only in one case this diagnosis was made praeoperatively, in 6 cases intraoperatively and in two cases only postoperatively. In the last two cases a reoperation was necessary. The surgical methods are described and the results of surgery are communicated.", "contents": "[Ventricular septal defect associated with fibrous subvalvular aortic stenosis: problems in the diagnosis and in the surgical management (author's transl)]. Only 9 of more than 900 operated patients with ventricular septal defects suffered from this rare combination. The problems of the diagnosis are discussed. Only in one case this diagnosis was made praeoperatively, in 6 cases intraoperatively and in two cases only postoperatively. In the last two cases a reoperation was necessary. The surgical methods are described and the results of surgery are communicated."}
{"id": "PMID:1081764", "title": "[The fate of the pulmonary hypertension after ASD-closure in adults (author's transl)].", "content": "10 female and 7 male patients, who were operated for secundum type Atrial Septal Defect with Pulmonary Hypertension, got postoperative check-ups including right heart catheterisations. The investigations were performed on the average 6.6 years postoperatively. There was a fall of the systolic pulmonary pressure and of the flow; pulmonary resistance fell in 8 cases and rose in 9. The low left ventricular cardiac index together with an elevated left atrial pressure may be the pathogenetic cause of postoperatively elevated pulmonary resistance.", "contents": "[The fate of the pulmonary hypertension after ASD-closure in adults (author's transl)]. 10 female and 7 male patients, who were operated for secundum type Atrial Septal Defect with Pulmonary Hypertension, got postoperative check-ups including right heart catheterisations. The investigations were performed on the average 6.6 years postoperatively. There was a fall of the systolic pulmonary pressure and of the flow; pulmonary resistance fell in 8 cases and rose in 9. The low left ventricular cardiac index together with an elevated left atrial pressure may be the pathogenetic cause of postoperatively elevated pulmonary resistance."}
{"id": "PMID:1081765", "title": "Late results of aortic homograft reconstruction of the right ventricular outflow tract in infants and children.", "content": "33 infants and children between 4 months and 14 years have undergone successful reconstruction of the right ventricular outflow tract with adult sized fresh aortic homografts. The late results have been analysed and it is concluded that satisfactory results can be obtained even in infants and that the operation should be performed early to prevent secondary changes in the heart and lungs.", "contents": "Late results of aortic homograft reconstruction of the right ventricular outflow tract in infants and children. 33 infants and children between 4 months and 14 years have undergone successful reconstruction of the right ventricular outflow tract with adult sized fresh aortic homografts. The late results have been analysed and it is concluded that satisfactory results can be obtained even in infants and that the operation should be performed early to prevent secondary changes in the heart and lungs."}
{"id": "PMID:1081766", "title": "[Critical outflow valve obstruction under the age of one year (author's transl)].", "content": "This report represents the Brompton Hospital experience of infants and neonates with critical pulmonary valve stenosis and aortic valve stenosis. There were 25 patients with critical pulmonary valve stenosis and 8 with critical aortic valve stenosis. Critical pulmonary stenosis: Among the 25 patients up to one year of age, 11 were girls and the commonest presenting symptom was cyanosis seen in 15, the commonest physical sign being a systolic ejection murmur with a single 2nd sound. A more detailed analysis of the symptoms, signs and findings at special investigation will be presented. The evolution of a satisfactory technique of operation will be indicated in these critically ill patients, and the late follow-up will be presented. Critical aortic stenosis: There were 8 patients in this group all of whom were male. Dyspnoea was the commonest presenting symptom with a systolic ejection murmur and hepatomegaly as the commonest signs. All patients in this group underwent open aortic valvotomy and/or valvuloplasty with 3 deaths. A late follow-up of the survivors will be presented. In summary, a series of 33 patients up to the age of one year with critical obstruction of either the pulmonary or aortic valves will be presented. (his is one of the larger series to be reported in this age group.", "contents": "[Critical outflow valve obstruction under the age of one year (author's transl)]. This report represents the Brompton Hospital experience of infants and neonates with critical pulmonary valve stenosis and aortic valve stenosis. There were 25 patients with critical pulmonary valve stenosis and 8 with critical aortic valve stenosis. Critical pulmonary stenosis: Among the 25 patients up to one year of age, 11 were girls and the commonest presenting symptom was cyanosis seen in 15, the commonest physical sign being a systolic ejection murmur with a single 2nd sound. A more detailed analysis of the symptoms, signs and findings at special investigation will be presented. The evolution of a satisfactory technique of operation will be indicated in these critically ill patients, and the late follow-up will be presented. Critical aortic stenosis: There were 8 patients in this group all of whom were male. Dyspnoea was the commonest presenting symptom with a systolic ejection murmur and hepatomegaly as the commonest signs. All patients in this group underwent open aortic valvotomy and/or valvuloplasty with 3 deaths. A late follow-up of the survivors will be presented. In summary, a series of 33 patients up to the age of one year with critical obstruction of either the pulmonary or aortic valves will be presented. (his is one of the larger series to be reported in this age group."}
{"id": "PMID:1081767", "title": "[Surgical treatment of kinking of the internal carotid artery (author's transl)].", "content": "Report on 20 cases of non arteriosclerotic kinking of the internal carotid artery. Besides the more common resection-technique a myoplasty using the digastric muscle is performed. Regional cerebral blood flow by 133 Xenon and angiography pre- and postoperatively are compared.", "contents": "[Surgical treatment of kinking of the internal carotid artery (author's transl)]. Report on 20 cases of non arteriosclerotic kinking of the internal carotid artery. Besides the more common resection-technique a myoplasty using the digastric muscle is performed. Regional cerebral blood flow by 133 Xenon and angiography pre- and postoperatively are compared."}
{"id": "PMID:1081768", "title": "[Nutritional effects following lumbar sympathectomy in arterial occlusive disease (author's transl)].", "content": "The effect of lumbar sympathectomy on regional blood-flow and on oxygen uptake of tissue has been investigated in animal experiments and in man. Lumbar sympathectomy did always result in increase of limb blood-flow, but did not significantly improve oxygen consumption of the observed extremity. This finding was manifested by marked narrowing of the arterial-venous oxyhemoglobin saturation difference suggesting that lumbar sympathectomy caused opening of arterial-venous shunts.", "contents": "[Nutritional effects following lumbar sympathectomy in arterial occlusive disease (author's transl)]. The effect of lumbar sympathectomy on regional blood-flow and on oxygen uptake of tissue has been investigated in animal experiments and in man. Lumbar sympathectomy did always result in increase of limb blood-flow, but did not significantly improve oxygen consumption of the observed extremity. This finding was manifested by marked narrowing of the arterial-venous oxyhemoglobin saturation difference suggesting that lumbar sympathectomy caused opening of arterial-venous shunts."}
{"id": "PMID:1081769", "title": "[Classification of mitral valve disease by clinical features and hemodynamic parameters (author's transl)].", "content": "Using the central venous oxygen saturation (SO2v) at rest and during exercise (50 Watt) a new classification of mitral valve disease is proposed, which corresponds with the recommendation of the NHA (1953). Contrary to the mostly subjective classification by clinical features, the new one results from objective hemodynamic parameters. The validity of the new proposed classification is shown using different clinical parameters.", "contents": "[Classification of mitral valve disease by clinical features and hemodynamic parameters (author's transl)]. Using the central venous oxygen saturation (SO2v) at rest and during exercise (50 Watt) a new classification of mitral valve disease is proposed, which corresponds with the recommendation of the NHA (1953). Contrary to the mostly subjective classification by clinical features, the new one results from objective hemodynamic parameters. The validity of the new proposed classification is shown using different clinical parameters."}
{"id": "PMID:1081770", "title": "[Operative results after mitral valve replacement with Starr-Edwards disc valves and Bj\u00f6rk-Shiley prostheses (author's transl)].", "content": "The postoperative course of 425 patients with isolated mitral valve replacement using either the Starr-Edwards disc prosthesis or the Bj\u00f6rk-Shiley tilting disc valve is evaluated. Both protheses showed comparable clinical results during a follow-up period between 5 months and 4 years.", "contents": "[Operative results after mitral valve replacement with Starr-Edwards disc valves and Bj\u00f6rk-Shiley prostheses (author's transl)]. The postoperative course of 425 patients with isolated mitral valve replacement using either the Starr-Edwards disc prosthesis or the Bj\u00f6rk-Shiley tilting disc valve is evaluated. Both protheses showed comparable clinical results during a follow-up period between 5 months and 4 years."}
{"id": "PMID:1081771", "title": "[1-Year-results of anuloplastisy (Carpentier-technic) in 7 patients (author's transl)].", "content": "Of 7 patients who had Carpentier anuloplasty, two had very good, two had good and two had unsatisfactory results in postoperative cardiac catheterization one year after operation. One patient died of acute hepatitis. Reasons for the poor results are false indications and inadequate operative technic. These results show that the Carpentier-technic can well be recommended for critical use in carefully selected patients with mitral insufficiency.", "contents": "[1-Year-results of anuloplastisy (Carpentier-technic) in 7 patients (author's transl)]. Of 7 patients who had Carpentier anuloplasty, two had very good, two had good and two had unsatisfactory results in postoperative cardiac catheterization one year after operation. One patient died of acute hepatitis. Reasons for the poor results are false indications and inadequate operative technic. These results show that the Carpentier-technic can well be recommended for critical use in carefully selected patients with mitral insufficiency."}
{"id": "PMID:1081772", "title": "[Aortic valve replacement for acute heart failure due to infective endocarditis (author's transl)].", "content": "5 patients with primary infective endocarditis developed acute cardiac failure because of aortic valve insufficiency. 2 patients were in the active and 3 patients in the inactive stage of infective endocarditis. In 4 patients an aortic and in 1 patient an aortic and mitral valve replacement was performed. The early mortality rate (death within 30 days of operation) was 20 per cent. Once congestive heart failure starts during the course of active endocarditis surgery should be undertaken at an early date.", "contents": "[Aortic valve replacement for acute heart failure due to infective endocarditis (author's transl)]. 5 patients with primary infective endocarditis developed acute cardiac failure because of aortic valve insufficiency. 2 patients were in the active and 3 patients in the inactive stage of infective endocarditis. In 4 patients an aortic and in 1 patient an aortic and mitral valve replacement was performed. The early mortality rate (death within 30 days of operation) was 20 per cent. Once congestive heart failure starts during the course of active endocarditis surgery should be undertaken at an early date."}
{"id": "PMID:1081773", "title": "[Surgical treatment of coronary heart disease (author's transl)].", "content": "Until 1974 146 patients underwent coronary surgery in Hamburg. The total mortality rate for the first 100 postoperative days was 16,3 per cent. In the last years the mortality was reduced to 14 per cent. This group included all different selective and emergency surgical procedures for coronary disease. Due to the different degree of the coronary disease and/or of the impairment of the left ventricular function the mortality ranged between 0 per cent and 35 per cent. Some clinical parameters influencing the mortality rate are discussed.", "contents": "[Surgical treatment of coronary heart disease (author's transl)]. Until 1974 146 patients underwent coronary surgery in Hamburg. The total mortality rate for the first 100 postoperative days was 16,3 per cent. In the last years the mortality was reduced to 14 per cent. This group included all different selective and emergency surgical procedures for coronary disease. Due to the different degree of the coronary disease and/or of the impairment of the left ventricular function the mortality ranged between 0 per cent and 35 per cent. Some clinical parameters influencing the mortality rate are discussed."}
{"id": "PMID:1081774", "title": "[The use of laser in thoracic-, cardiac- and vascular surgery (author's transl)].", "content": "We carried out thoracic and cardiovascular operations with a 50 watt CO2-Laser. The advantage of this procedure: A minimal trauma of tissue in consequence of no use of selfcutting surgical instruments, bloodless incision at smallest area of coagulation and no possibility of displacement of infections and tumor cells. We believe that this new laser in all surgical disciplines introduced soon.", "contents": "[The use of laser in thoracic-, cardiac- and vascular surgery (author's transl)]. We carried out thoracic and cardiovascular operations with a 50 watt CO2-Laser. The advantage of this procedure: A minimal trauma of tissue in consequence of no use of selfcutting surgical instruments, bloodless incision at smallest area of coagulation and no possibility of displacement of infections and tumor cells. We believe that this new laser in all surgical disciplines introduced soon."}
{"id": "PMID:1081775", "title": "[Is transvenous cardiac pacemaker implantation with X-ray control associated with a dangerous radiation exposure? (author's transl)].", "content": "From July to December 1974 we measured the radiation exposure of the hands of the operator and assistant with 69 implantations of permanent cardiac pacemakers and recorded a dose of 0,5 mrem/min, this corresponds to 4 mrem/patient.", "contents": "[Is transvenous cardiac pacemaker implantation with X-ray control associated with a dangerous radiation exposure? (author's transl)]. From July to December 1974 we measured the radiation exposure of the hands of the operator and assistant with 69 implantations of permanent cardiac pacemakers and recorded a dose of 0,5 mrem/min, this corresponds to 4 mrem/patient."}
{"id": "PMID:1081776", "title": "A prospective study of HL-A antigen phenotypes and lymphocyte abnormalities in Hodgkin's disease.", "content": "In a prospective study, sixty-five histologically verified Swedish cases of Hodgkin's disease were HL-A typed. The antigen frequencies in the patient group did not deviate from a group of normal controls. The frequency of HL-A8 was slightly increased in patients over 40 years (P less than 0.05). HL-A12 was markedly increased in patients below 40 years with a favourable histopathology (P less than 0.001). No association between sex or clinical stage and distribution of HL-A phenotypes was observed. The number of T-lymphocytes in blood and the spontaneous DNA synthesis and DNA synthesis induced by concanavalin A, pokeweed mitogen, phytohemagglutinin and PPD antigen in blood lymphocytes in vitro were determined in 60 patients. A significant increase of HL-A28 was noted in patients with advanced disease (P less than 0.01) and in patients responding poorly to PPD (P less than 0.001) and PWM (P less than 0.01). No correlation between HL-A antigen distribution and T-lymphocyte counts was observed.", "contents": "A prospective study of HL-A antigen phenotypes and lymphocyte abnormalities in Hodgkin's disease. In a prospective study, sixty-five histologically verified Swedish cases of Hodgkin's disease were HL-A typed. The antigen frequencies in the patient group did not deviate from a group of normal controls. The frequency of HL-A8 was slightly increased in patients over 40 years (P less than 0.05). HL-A12 was markedly increased in patients below 40 years with a favourable histopathology (P less than 0.001). No association between sex or clinical stage and distribution of HL-A phenotypes was observed. The number of T-lymphocytes in blood and the spontaneous DNA synthesis and DNA synthesis induced by concanavalin A, pokeweed mitogen, phytohemagglutinin and PPD antigen in blood lymphocytes in vitro were determined in 60 patients. A significant increase of HL-A28 was noted in patients with advanced disease (P less than 0.01) and in patients responding poorly to PPD (P less than 0.001) and PWM (P less than 0.01). No correlation between HL-A antigen distribution and T-lymphocyte counts was observed."}
{"id": "PMID:1081778", "title": "[Emergency direct revascularization of the myocardium in acute infarct].", "content": "A new surgical conception of urgent direct revascularization of the myocardium in acute infarction is discussed and substantiated. Clinical observations and results of 13 urgent operations for aorto-coronary shunting in 10 patients with acute myocardial infarction are analysed (double aorto-coronary shunting was performed in 3 cases). Indications to urgent revascularization of the myocardium in infarction were as follows: no effect of drug therapy during 2--3 hours since its onset, a failure to eliminate pulmonary edema and cardiogenic shock in a localized proximal occlusion of the coronary artery, revealed during a planned or urgent coronarography. Three patients were operated upon in the state of cardiogenic shock (one after reanimation), two with pulmonary edema. All these patients (excluding one with pulmonary edema) have made a good recovery. Three patients died. Good immediate and late results obtained evidence the advantage of this policy. The problems of most optimum terms for urgent revascularization of the myocardium are discussed.", "contents": "[Emergency direct revascularization of the myocardium in acute infarct]. A new surgical conception of urgent direct revascularization of the myocardium in acute infarction is discussed and substantiated. Clinical observations and results of 13 urgent operations for aorto-coronary shunting in 10 patients with acute myocardial infarction are analysed (double aorto-coronary shunting was performed in 3 cases). Indications to urgent revascularization of the myocardium in infarction were as follows: no effect of drug therapy during 2--3 hours since its onset, a failure to eliminate pulmonary edema and cardiogenic shock in a localized proximal occlusion of the coronary artery, revealed during a planned or urgent coronarography. Three patients were operated upon in the state of cardiogenic shock (one after reanimation), two with pulmonary edema. All these patients (excluding one with pulmonary edema) have made a good recovery. Three patients died. Good immediate and late results obtained evidence the advantage of this policy. The problems of most optimum terms for urgent revascularization of the myocardium are discussed."}
{"id": "PMID:1081779", "title": "[Importance of the type of blood supply to the heart in reconstructive surgery of the coronary arteries].", "content": "Anatomical data on the type of the heart blood supply are reported. Based on anatomical studies on 35 hearts, the author found the right type of blood supply in 24 cases, the left type in 4, and balanced in 7 cases. The study of coronarograms of 100 operated patients and data of the intraoperative diagnosis indicated that anatomical variants of coronary arteries extension fully agree with coronarography findings. At the same time there was no definite dependence between the type of cardiac blood supply and the degree and character of coronary arteries affection.", "contents": "[Importance of the type of blood supply to the heart in reconstructive surgery of the coronary arteries]. Anatomical data on the type of the heart blood supply are reported. Based on anatomical studies on 35 hearts, the author found the right type of blood supply in 24 cases, the left type in 4, and balanced in 7 cases. The study of coronarograms of 100 operated patients and data of the intraoperative diagnosis indicated that anatomical variants of coronary arteries extension fully agree with coronarography findings. At the same time there was no definite dependence between the type of cardiac blood supply and the degree and character of coronary arteries affection."}
{"id": "PMID:1081777", "title": "A case report of a hemolytic transfusion reaction caused by anti-Holley.", "content": "Clinical and laboratory investigation of a black male patient, who received emergency transfusion of blood incompatible for a high incidence antigen, provided evidence for the pathogenetic importance of high titer anti-Holley antibodies with poor avidity. The red blood cells of the patient and two siblings were remarkable in being negative for two high incidence antigens, Hy and hrS, and weak for a third, Gy.", "contents": "A case report of a hemolytic transfusion reaction caused by anti-Holley. Clinical and laboratory investigation of a black male patient, who received emergency transfusion of blood incompatible for a high incidence antigen, provided evidence for the pathogenetic importance of high titer anti-Holley antibodies with poor avidity. The red blood cells of the patient and two siblings were remarkable in being negative for two high incidence antigens, Hy and hrS, and weak for a third, Gy."}
{"id": "PMID:1081784", "title": "[X-ray findings in meningiomas of the tentorium cerebelli].", "content": "A roentgenological investigation of 38 patients with meningiomas of the tentorium cerebelli of the supra-subtentorial growth revealed signs of hypertension in 31 of them. The destruction of the squama occipitalis and of the superior edge of the apex of the petrous pyramid was found in 17 of these patients. Opacification of the ventricular system discerned a combination that included compression of the inferior contour of the posterior segments of the lateral ventricles or a filling defect of the trigonum of the left ventricle with deformation of the posterior segment of the III ventricle and a displacement forward of the aqueductus Sylvii. An angiographic investigation of the carotid and basilar systems determine a typical picture of simultaneous shifts, viz, upwards--of the median cerebral artery, medially--of the internal occipital artery; outwards--of the temporal-occipital artery, medially and downwards--of the superior cerebral artery. Obliteration of the sinuses rectus and transversalis comes into evidence. Opacification of the meningioma vasculature above and underneath the tentorium cerebelli makes the topical diagnosis quite convincing.", "contents": "[X-ray findings in meningiomas of the tentorium cerebelli]. A roentgenological investigation of 38 patients with meningiomas of the tentorium cerebelli of the supra-subtentorial growth revealed signs of hypertension in 31 of them. The destruction of the squama occipitalis and of the superior edge of the apex of the petrous pyramid was found in 17 of these patients. Opacification of the ventricular system discerned a combination that included compression of the inferior contour of the posterior segments of the lateral ventricles or a filling defect of the trigonum of the left ventricle with deformation of the posterior segment of the III ventricle and a displacement forward of the aqueductus Sylvii. An angiographic investigation of the carotid and basilar systems determine a typical picture of simultaneous shifts, viz, upwards--of the median cerebral artery, medially--of the internal occipital artery; outwards--of the temporal-occipital artery, medially and downwards--of the superior cerebral artery. Obliteration of the sinuses rectus and transversalis comes into evidence. Opacification of the meningioma vasculature above and underneath the tentorium cerebelli makes the topical diagnosis quite convincing."}
{"id": "PMID:1081785", "title": "[Eye movements in animals with extirpated cerebrocortical oculomotor centers].", "content": "An investigation of dogs with removed temporal and sincipito-occipital cortical centres of the eye movements demonstrated that a number of properties of the vestibular nystagmus evoked by the rotation of the animal remain the same as they had been in this very same animal prior to the operation. These centres do not control the nature of the nystagmic movement that consists of a slow and a fast components, the combined movements of the right and left eyes, the direction of the nystagmus, the range and the nature marking the distribution of the maximal movement and of the most frequent movements during the action of the stimulus and the symmetry of the labyrinthine function. At the same time, the dissociation of the cortical centres of the eye movements brings on shortening of the vestibular post-rotational nystagmus, activation of the mechanism that monitors the rhythmic tonic deflections of the eyes, this causing diminution in the number of nystagmic eye movements and disruption in the rhythm of their movement. It also activates arbitrary movements of the eyes that occasionally turn into a spontaneous nystagmus. The abundance of arbitrary eye movements following vestibular stimulation is of frequent occurrence in children, victims of cerebral paralysis of childhood combined wit- depressed cortical function.", "contents": "[Eye movements in animals with extirpated cerebrocortical oculomotor centers]. An investigation of dogs with removed temporal and sincipito-occipital cortical centres of the eye movements demonstrated that a number of properties of the vestibular nystagmus evoked by the rotation of the animal remain the same as they had been in this very same animal prior to the operation. These centres do not control the nature of the nystagmic movement that consists of a slow and a fast components, the combined movements of the right and left eyes, the direction of the nystagmus, the range and the nature marking the distribution of the maximal movement and of the most frequent movements during the action of the stimulus and the symmetry of the labyrinthine function. At the same time, the dissociation of the cortical centres of the eye movements brings on shortening of the vestibular post-rotational nystagmus, activation of the mechanism that monitors the rhythmic tonic deflections of the eyes, this causing diminution in the number of nystagmic eye movements and disruption in the rhythm of their movement. It also activates arbitrary movements of the eyes that occasionally turn into a spontaneous nystagmus. The abundance of arbitrary eye movements following vestibular stimulation is of frequent occurrence in children, victims of cerebral paralysis of childhood combined wit- depressed cortical function."}
{"id": "PMID:1081791", "title": "[Complete gastric erosions--a relatively frequent gastroscopy finding].", "content": "While by means of the semiflexible Wolf-Schnidler-gastroscope no complete erosions had been seen, they were found in 12.7% in 615 gastroscopies, carried out with glass fibre endoscopes. In nearly 80% of the patients the erosions, appearing in most cases in a larger number, were localised in the antrum ventriculi. As the most frequent concomitant disease the gastroduodenal ulcer was found. Roentgenologically in no case the diagnosis erosion was made. As to the soft tissues defects of the epithelium could rarely be ascertained, however, more frequently a foveolar pseudohyperplasia as the morphological substrate of the marginal wall. Chronic-atrophic changes of the mucous membrane in the environment of the erosions were by far more frequently to be proved than superficial gastritides or normal findings on the mucous membrane of the stomach.", "contents": "[Complete gastric erosions--a relatively frequent gastroscopy finding]. While by means of the semiflexible Wolf-Schnidler-gastroscope no complete erosions had been seen, they were found in 12.7% in 615 gastroscopies, carried out with glass fibre endoscopes. In nearly 80% of the patients the erosions, appearing in most cases in a larger number, were localised in the antrum ventriculi. As the most frequent concomitant disease the gastroduodenal ulcer was found. Roentgenologically in no case the diagnosis erosion was made. As to the soft tissues defects of the epithelium could rarely be ascertained, however, more frequently a foveolar pseudohyperplasia as the morphological substrate of the marginal wall. Chronic-atrophic changes of the mucous membrane in the environment of the erosions were by far more frequently to be proved than superficial gastritides or normal findings on the mucous membrane of the stomach."}
{"id": "PMID:1081792", "title": "[Glycoproteins in diabetes mellitus].", "content": "Analyses of mucoproteins, orcin-positive protein sugar, sialic acid of the alpha2-macroglobulin, alpha1-glycoprotein, alpha1-antitrypsin, alpha2-HS-glycoprotein and beta2-glycoprotein I were carried out in 302 diabetics. In creases of concentration could be proved for alpha2-macroglobulin, alpha2-HS-glycoprotein, orcin-positive protein sugar and sialic acid. In diabetics with retinopathies the alpha2-macroglobulin concentration is significantly increased compared with diabetics without retinopathies.", "contents": "[Glycoproteins in diabetes mellitus]. Analyses of mucoproteins, orcin-positive protein sugar, sialic acid of the alpha2-macroglobulin, alpha1-glycoprotein, alpha1-antitrypsin, alpha2-HS-glycoprotein and beta2-glycoprotein I were carried out in 302 diabetics. In creases of concentration could be proved for alpha2-macroglobulin, alpha2-HS-glycoprotein, orcin-positive protein sugar and sialic acid. In diabetics with retinopathies the alpha2-macroglobulin concentration is significantly increased compared with diabetics without retinopathies."}
{"id": "PMID:1081793", "title": "[Stomach ulcer in the emergency endoscopy].", "content": "After explanation of the diagnostic gain in acute gastrointestinal haemorrhages by use of the emergency endoscopy the results and experiences are reported got in 200 emergency examinations. As cause of haemorrhage most frequency were found peptic ulcers followed by gastric erosions. In one third the erosions were associated with other sources of haemorrhage and should be accepted as cause of haemorrhage only with cautiousness. Flat acute ulcerations cannot be diagnosed endoscopically already by relatively thin-layered linings and chronic ulcerations by plications.", "contents": "[Stomach ulcer in the emergency endoscopy]. After explanation of the diagnostic gain in acute gastrointestinal haemorrhages by use of the emergency endoscopy the results and experiences are reported got in 200 emergency examinations. As cause of haemorrhage most frequency were found peptic ulcers followed by gastric erosions. In one third the erosions were associated with other sources of haemorrhage and should be accepted as cause of haemorrhage only with cautiousness. Flat acute ulcerations cannot be diagnosed endoscopically already by relatively thin-layered linings and chronic ulcerations by plications."}
{"id": "PMID:1081797", "title": "The aortogram and gastrointestinal bleeding.", "content": "Three cases of gastrointestinal hemorrhage are reported which illustrate that the bleeding site may be missed angiographically if the study is limited to celiac and mesenteric angiograms. An abdominal angiogram must be performed as part of the radiological investigation of gastrointestinal bleeding.", "contents": "The aortogram and gastrointestinal bleeding. Three cases of gastrointestinal hemorrhage are reported which illustrate that the bleeding site may be missed angiographically if the study is limited to celiac and mesenteric angiograms. An abdominal angiogram must be performed as part of the radiological investigation of gastrointestinal bleeding."}
{"id": "PMID:1081798", "title": "Coronary graft flow and glucose tolerance: evidence against the existence of myocardial microvascular disease.", "content": "Patients having coronary bypass for stable angina pectoris were grouped on the basis of the two hour plasma sugar of the glucose tolerance test: Group I, 120 mgs% (159 grafts); Group II, 120-150 (93 grafts); Group III, 150-200 (131 grafts) and Group IV, 200 (57 grafts) or patients receiving therapy for diabetes mellitus (10 patients, 21 grafts). Five of 10 diabetic patients had genetic evidence of diabetes and an average duration of therapy of 6.5 years. Blood flow was measured in 461 grafts with an electromagnetic flow probe after discontinuation of cardiopulmonary bypass in a stable state, after a 30 second graft occlusion and after injection of 15 mg of papaverine into the graft. Mean arterial pressure, graft flow and coronary resistance for each succeeding group did not vary significantly when compared with Group I. Analysis of phasic flow in 10 grafts to the left anterior descending indicates that the same proportion of flow occurs during systole and diastole in the basal state and after pappaverine. Coronary flow and resistance in patients with abnormal glucose metabolism and maturity onset diabetes do not provide evidence for the existence of myocardial microangiopathy.", "contents": "Coronary graft flow and glucose tolerance: evidence against the existence of myocardial microvascular disease. Patients having coronary bypass for stable angina pectoris were grouped on the basis of the two hour plasma sugar of the glucose tolerance test: Group I, 120 mgs% (159 grafts); Group II, 120-150 (93 grafts); Group III, 150-200 (131 grafts) and Group IV, 200 (57 grafts) or patients receiving therapy for diabetes mellitus (10 patients, 21 grafts). Five of 10 diabetic patients had genetic evidence of diabetes and an average duration of therapy of 6.5 years. Blood flow was measured in 461 grafts with an electromagnetic flow probe after discontinuation of cardiopulmonary bypass in a stable state, after a 30 second graft occlusion and after injection of 15 mg of papaverine into the graft. Mean arterial pressure, graft flow and coronary resistance for each succeeding group did not vary significantly when compared with Group I. Analysis of phasic flow in 10 grafts to the left anterior descending indicates that the same proportion of flow occurs during systole and diastole in the basal state and after pappaverine. Coronary flow and resistance in patients with abnormal glucose metabolism and maturity onset diabetes do not provide evidence for the existence of myocardial microangiopathy."}
{"id": "PMID:1081799", "title": "Aneurysms of an aorto-coronary artery saphenous vein bypass graft--a case report.", "content": "A 62 year old man with severe angina pectoris underwent aoroto-coronary saphenous vein graft implantation. After a four month asymptomatic period the patient underwent aorto-coronary graft angiography. Left anterior descending graft opacification demonstrated multiple venous aneurysmal dilations. The clinical significance of this finding is currently unknown but such saphenous vein disease warrants further investigation. Saphenous vein grafts have been utilized to replace or bypass obstructed and diseased peripheral arteries such as those of the carotid and femoral-popliteal vascular systesm. In 1967 Favaloro introduced the saphenous vein as a means for bypassing local obstructions in diseased coronary arteries of human subjects. Since the advent of the aorto-coronary saphenous vein bypass graft procedure several studies have reported pathologic alterations of these grafts at post-mortem examination or at the time of re-operation. Other investigators have also noted changes in these venous grafts when they have been implanted in peripheral vessels. We report here a patient with aneruysmal dilations of an aorto-coronary artery saphenous vein bypass graft. Furthermore, possible mechanisms which might produce such venous aneurysms and the clinical significance of such an entity are discussed.", "contents": "Aneurysms of an aorto-coronary artery saphenous vein bypass graft--a case report. A 62 year old man with severe angina pectoris underwent aoroto-coronary saphenous vein graft implantation. After a four month asymptomatic period the patient underwent aorto-coronary graft angiography. Left anterior descending graft opacification demonstrated multiple venous aneurysmal dilations. The clinical significance of this finding is currently unknown but such saphenous vein disease warrants further investigation. Saphenous vein grafts have been utilized to replace or bypass obstructed and diseased peripheral arteries such as those of the carotid and femoral-popliteal vascular systesm. In 1967 Favaloro introduced the saphenous vein as a means for bypassing local obstructions in diseased coronary arteries of human subjects. Since the advent of the aorto-coronary saphenous vein bypass graft procedure several studies have reported pathologic alterations of these grafts at post-mortem examination or at the time of re-operation. Other investigators have also noted changes in these venous grafts when they have been implanted in peripheral vessels. We report here a patient with aneruysmal dilations of an aorto-coronary artery saphenous vein bypass graft. Furthermore, possible mechanisms which might produce such venous aneurysms and the clinical significance of such an entity are discussed."}
{"id": "PMID:1081804", "title": "[Characteristics of some species of microorganisms related to the genus of hemoglobinophils].", "content": "The saccharolytic capacity in respect to 14 carbohydrates, the lipolytic activity, the presence of urease, catalase, galactosidase, the formation of hydrogen sulphide and indol and also serological properties with the species agglutinating sera were studied in the representatives of the genus of hemoglobinophilic microbes: H. influenzae, H. parainfluenzae, H. aegiptius, H. haemolyticus, H. aphrophilus. There were revealed differences in the individual representatives of the genus by the enzymatic activity and serological properties. Thus, representatives of H. influenzae possessed urease activity, but all of them lacked galactosidase. H. aegiptius possessed urease and galactosidase, and H. parainfluenzae, H. haemolyticus and H. aphrophilus--galactosidase of high activity, but no urease. Representatives of each of the species were agglutinated by homologous sera only.", "contents": "[Characteristics of some species of microorganisms related to the genus of hemoglobinophils]. The saccharolytic capacity in respect to 14 carbohydrates, the lipolytic activity, the presence of urease, catalase, galactosidase, the formation of hydrogen sulphide and indol and also serological properties with the species agglutinating sera were studied in the representatives of the genus of hemoglobinophilic microbes: H. influenzae, H. parainfluenzae, H. aegiptius, H. haemolyticus, H. aphrophilus. There were revealed differences in the individual representatives of the genus by the enzymatic activity and serological properties. Thus, representatives of H. influenzae possessed urease activity, but all of them lacked galactosidase. H. aegiptius possessed urease and galactosidase, and H. parainfluenzae, H. haemolyticus and H. aphrophilus--galactosidase of high activity, but no urease. Representatives of each of the species were agglutinated by homologous sera only."}
{"id": "PMID:1081805", "title": "[Interaction of various types of cells in the production of anti-influenzal antibodies. III. Cooperation of B- and T-lymphocytes in the production of antibodies to the influenza virus].", "content": "Bone marrow and thymus cells of syngenous donors were transplanted to sublethally irradiated mice to study the interaction between the B- and T-lymphocytes in the production of antibodies to the influenza virus. Influenza virus proved to be referred to the thymus-dependent antigens; both types of the lymphocytes participated in the antibody production. The T-lymphocyte function could not be replaced by an increase in the dose of the antigen and of the amount of B-lymphocytes. The ratio of the B- and T-lymphocytes optimal for the antibody production was 1:1 or 1:2.", "contents": "[Interaction of various types of cells in the production of anti-influenzal antibodies. III. Cooperation of B- and T-lymphocytes in the production of antibodies to the influenza virus]. Bone marrow and thymus cells of syngenous donors were transplanted to sublethally irradiated mice to study the interaction between the B- and T-lymphocytes in the production of antibodies to the influenza virus. Influenza virus proved to be referred to the thymus-dependent antigens; both types of the lymphocytes participated in the antibody production. The T-lymphocyte function could not be replaced by an increase in the dose of the antigen and of the amount of B-lymphocytes. The ratio of the B- and T-lymphocytes optimal for the antibody production was 1:1 or 1:2."}
{"id": "PMID:1081806", "title": "[Purification of an anionic trypsin from the gastric juice of the crayfish Cambarus affinis say by means of affinity chromatography].", "content": "Beside other digestion enzymes the crayfish Cambarus affinis SAY possess an anionic trypsin. This enzyme could be separated from all the other proteins of the crude gastric juice by a single affinity chromatographic step on immobilized natural inhibitor. The dissociation of the thus produced enzyme-inhibitor complex was achieved by specific elution with ben-amidine. The soluble benzamidine-trypsin-complex was dialyzed and separated by gelfiltration on Sephadex G-50. Of the ligands used Trasylol proved to be the most effective one. Trypsin isolated by this ligand was in a discelectrophoretic homogeneous state. On the other hand, trypsin purified by Soybean trypsin inhibitor and Contrykal contains impurities.", "contents": "[Purification of an anionic trypsin from the gastric juice of the crayfish Cambarus affinis say by means of affinity chromatography]. Beside other digestion enzymes the crayfish Cambarus affinis SAY possess an anionic trypsin. This enzyme could be separated from all the other proteins of the crude gastric juice by a single affinity chromatographic step on immobilized natural inhibitor. The dissociation of the thus produced enzyme-inhibitor complex was achieved by specific elution with ben-amidine. The soluble benzamidine-trypsin-complex was dialyzed and separated by gelfiltration on Sephadex G-50. Of the ligands used Trasylol proved to be the most effective one. Trypsin isolated by this ligand was in a discelectrophoretic homogeneous state. On the other hand, trypsin purified by Soybean trypsin inhibitor and Contrykal contains impurities."}
{"id": "PMID:1081807", "title": "[Oeso-portal disconnection by ligature, segmental resection and anastomosis of abdominal oesophagus by means of the PKS 25 apparatus in the cirrhotic patient (author's transl)].", "content": "After a brief report of the different techniques of ligating abdominal oesophagus recommended by Vosschulte, Boerema Prioton..., the author describes his technique for resection-anastomosis with the PKS 25 apparatus. He makes a critical analysis of the method: this seems to avoid the drawbacks of the rigid, metallic ring with its narrow bore and uncertain elimination through the natural channel. By the same occasion it respects the principles, advantages and indications of the preceding methods. The author ends by the main therapeutic choices that appear to emerge for this treatment of oesophageal varices by opposition to the portal decompression operations, be it during hemorrhage or delayed emergency.", "contents": "[Oeso-portal disconnection by ligature, segmental resection and anastomosis of abdominal oesophagus by means of the PKS 25 apparatus in the cirrhotic patient (author's transl)]. After a brief report of the different techniques of ligating abdominal oesophagus recommended by Vosschulte, Boerema Prioton..., the author describes his technique for resection-anastomosis with the PKS 25 apparatus. He makes a critical analysis of the method: this seems to avoid the drawbacks of the rigid, metallic ring with its narrow bore and uncertain elimination through the natural channel. By the same occasion it respects the principles, advantages and indications of the preceding methods. The author ends by the main therapeutic choices that appear to emerge for this treatment of oesophageal varices by opposition to the portal decompression operations, be it during hemorrhage or delayed emergency."}
{"id": "PMID:1081808", "title": "Malignant fibrous mesothelioma. Metastatic to brain and liver.", "content": "A malignant fibrous mesothelioma in a 52 year old white man arising from the left parietal pleura associated with lupus erythematosus with metastases to brain and liver is reported. Asbestos bodies were found in digested pulmonary tissue but none in the primary or metastatic lesions. Light microscopic and ultrastructural studies suggest that this tumor contains mesothelial or endothelial cells, some of which revealed fibroblastic features while others disclosed epitheloid characteristics.", "contents": "Malignant fibrous mesothelioma. Metastatic to brain and liver. A malignant fibrous mesothelioma in a 52 year old white man arising from the left parietal pleura associated with lupus erythematosus with metastases to brain and liver is reported. Asbestos bodies were found in digested pulmonary tissue but none in the primary or metastatic lesions. Light microscopic and ultrastructural studies suggest that this tumor contains mesothelial or endothelial cells, some of which revealed fibroblastic features while others disclosed epitheloid characteristics."}
{"id": "PMID:1081809", "title": "Studies on coagulation and fibrinolysis in pregnancy, with special reference to cold activation of factor VII.", "content": "Studies on coagulation and fibrinolysis were performed on 40 healthy women who underwent an uncomplicated pregnancy. From each subject, samples were taken 6-10 times during pregnancy, and all the changes were related to the subjects' own 6-week postpartum values. Increased concentration of coagulation factors II-VII-X was revealed by Normotest. The incidence of cold activation of factor VII increased from 14% in non-pregnant women in these series to 94% in pregnant women near term. A marked increase in the proteolytic capacity was probably an effect of increased plasminogen concentration and decreased antifibrinolytic activity. The latter did not, however, parallel the immunological determination of the antiplasmins. The concentrations of antithrombin III and CI inactivator of complement both decreased during pregnancy. It is concluded that the overall effect of these changes is probably in favour of coagulation.", "contents": "Studies on coagulation and fibrinolysis in pregnancy, with special reference to cold activation of factor VII. Studies on coagulation and fibrinolysis were performed on 40 healthy women who underwent an uncomplicated pregnancy. From each subject, samples were taken 6-10 times during pregnancy, and all the changes were related to the subjects' own 6-week postpartum values. Increased concentration of coagulation factors II-VII-X was revealed by Normotest. The incidence of cold activation of factor VII increased from 14% in non-pregnant women in these series to 94% in pregnant women near term. A marked increase in the proteolytic capacity was probably an effect of increased plasminogen concentration and decreased antifibrinolytic activity. The latter did not, however, parallel the immunological determination of the antiplasmins. The concentrations of antithrombin III and CI inactivator of complement both decreased during pregnancy. It is concluded that the overall effect of these changes is probably in favour of coagulation."}
{"id": "PMID:1081812", "title": "The ultrastructure of Trichomonas vaginalis donn\u00e9 before and after transfer from vaginal secretion to Diamonds medium.", "content": "Cell of Trichomonas vaginalis (T. vag.) obtained from five patients with severe trichomoniasis were fixed for electron microscopy directly in the vaginal fluid. Samples of the same material from three of the patients was inoculated into Diamonds medium and propagated for 3-6 weeks. During the first 3-4 weeks in vitro the fine structure of all cells was changed. The shape of the nucleus, the size of the Golgi apparatus and adjacent vesicles, the fine structure of the chromatic granules, and the mastigont were all changed when compared to the organelles of the originally isolated cells. In addition the \"ectoplasm\" had disappeared, the glycogen granules had become sparse, and the number of free cytoplasmic ribosomes had increased. On the other hand, the large food vacuoles and the cell membranes generally were unchanged. Mycoplasms were observed in the original isolate and were also seen after more than 6 weeks of propagation, both as free organisms in the culture medium and within the large food vacuoles of \"in vitro\" cultured cells.", "contents": "The ultrastructure of Trichomonas vaginalis donn\u00e9 before and after transfer from vaginal secretion to Diamonds medium. Cell of Trichomonas vaginalis (T. vag.) obtained from five patients with severe trichomoniasis were fixed for electron microscopy directly in the vaginal fluid. Samples of the same material from three of the patients was inoculated into Diamonds medium and propagated for 3-6 weeks. During the first 3-4 weeks in vitro the fine structure of all cells was changed. The shape of the nucleus, the size of the Golgi apparatus and adjacent vesicles, the fine structure of the chromatic granules, and the mastigont were all changed when compared to the organelles of the originally isolated cells. In addition the \"ectoplasm\" had disappeared, the glycogen granules had become sparse, and the number of free cytoplasmic ribosomes had increased. On the other hand, the large food vacuoles and the cell membranes generally were unchanged. Mycoplasms were observed in the original isolate and were also seen after more than 6 weeks of propagation, both as free organisms in the culture medium and within the large food vacuoles of \"in vitro\" cultured cells."}
{"id": "PMID:1081810", "title": "An extended approach through the middle cranial fossa to the internal auditory meatus and the cerebello-pontine angle.", "content": "The submeningeal approach through the middle cranial fossa to the internal auditory meatus and cerebello-pontine angle after cutting the tentorium cerebelli is described. It is in some way similar to that used by Morrison & King. The approach is achieved by drilling the petrous bone to the level of the compact plate of the sigmoid sinus sulcus and to the lumen of the lateral semicircular canal. In this way it is possible to visualize safely the entire auditory-facial fasciculus, the region of the jugular foramen, the IX, X, XI, V, and VI nerves as well as the lateral surface of the brain stem. In our opinion this way should be and is preferable to the translabyrinthine approach through the mastoid process.", "contents": "An extended approach through the middle cranial fossa to the internal auditory meatus and the cerebello-pontine angle. The submeningeal approach through the middle cranial fossa to the internal auditory meatus and cerebello-pontine angle after cutting the tentorium cerebelli is described. It is in some way similar to that used by Morrison & King. The approach is achieved by drilling the petrous bone to the level of the compact plate of the sigmoid sinus sulcus and to the lumen of the lateral semicircular canal. In this way it is possible to visualize safely the entire auditory-facial fasciculus, the region of the jugular foramen, the IX, X, XI, V, and VI nerves as well as the lateral surface of the brain stem. In our opinion this way should be and is preferable to the translabyrinthine approach through the mastoid process."}
{"id": "PMID:1081811", "title": "Immunological function of human tonsil. Surface topology of human tonsil lymphocytes using the scanning electron microscope (SEM).", "content": "The surface topology of the lymphocytes in human tonsils was performed by means of the scanning electron microscope (SEM). It was revealed that human tonsils consist of both 40.7% villous and 26.6% smooth surface lymphocytes. In general, the percentage results agree with those of E and EAC binding lymphocytes of human tonsils, as previously reported. Our serial experiments using immunological techniques proved the existence of two lymphocytes (T and B cell) having immunological roles.", "contents": "Immunological function of human tonsil. Surface topology of human tonsil lymphocytes using the scanning electron microscope (SEM). The surface topology of the lymphocytes in human tonsils was performed by means of the scanning electron microscope (SEM). It was revealed that human tonsils consist of both 40.7% villous and 26.6% smooth surface lymphocytes. In general, the percentage results agree with those of E and EAC binding lymphocytes of human tonsils, as previously reported. Our serial experiments using immunological techniques proved the existence of two lymphocytes (T and B cell) having immunological roles."}
{"id": "PMID:1081814", "title": "Seventy-year-old people in Gothenburg. A population study in an industrialized Swedish city.", "content": "The objectives of this cross-sectional population study of 70-year-old people in Gothenburg, Sweden, were to make a survey of the social and medical conditions of the population, to obtain basic data for planning the care of the elderly, to contribute to the knowledge of normal ageing processes and of normal criteria within the age group, and to offer the subjects a thorough medical examination. A representative systematic sample comprised 1 148 propositi (521 males and 627 females). The study comprised a homecall part regarding basic personal data, dwelling conditions, economy, social and physical communications, previous migration, educational level, previous and any present professions, need for care, consumption of health care and drugs. Observations and measurements were done of illumination at the habitual site of reading, and a simple test of visual acuity was performed. Later on, examinations at the Out-patient Department of the Geriatric Hospital were performed, comprising in all probands general medical and ophthalmological examination, blood and urine sampling, ECG and X-ray of heart, lungs, and breasts and on subsamples, psychological, psychiatric, dental, somatometric, and audiometric examination. Furthermore, a dietary interview and a body composition examination were performed on subsamples. This first presentation deals with the design and procedure of the study, analysis of observer variation and a comparison between responders (85%) and non-responders (15%). The general design of the study, the sampling procedure, and general methods of data collection were found to be adequate. The responders were found to be representative for the population as a whole in most respects studied. The results of this study to be reported in subsequent papers will in general allow conclusions regarding the population of 70-year-olds in Gothenburg.", "contents": "Seventy-year-old people in Gothenburg. A population study in an industrialized Swedish city. The objectives of this cross-sectional population study of 70-year-old people in Gothenburg, Sweden, were to make a survey of the social and medical conditions of the population, to obtain basic data for planning the care of the elderly, to contribute to the knowledge of normal ageing processes and of normal criteria within the age group, and to offer the subjects a thorough medical examination. A representative systematic sample comprised 1 148 propositi (521 males and 627 females). The study comprised a homecall part regarding basic personal data, dwelling conditions, economy, social and physical communications, previous migration, educational level, previous and any present professions, need for care, consumption of health care and drugs. Observations and measurements were done of illumination at the habitual site of reading, and a simple test of visual acuity was performed. Later on, examinations at the Out-patient Department of the Geriatric Hospital were performed, comprising in all probands general medical and ophthalmological examination, blood and urine sampling, ECG and X-ray of heart, lungs, and breasts and on subsamples, psychological, psychiatric, dental, somatometric, and audiometric examination. Furthermore, a dietary interview and a body composition examination were performed on subsamples. This first presentation deals with the design and procedure of the study, analysis of observer variation and a comparison between responders (85%) and non-responders (15%). The general design of the study, the sampling procedure, and general methods of data collection were found to be adequate. The responders were found to be representative for the population as a whole in most respects studied. The results of this study to be reported in subsequent papers will in general allow conclusions regarding the population of 70-year-olds in Gothenburg."}
{"id": "PMID:1081815", "title": "Preformed mRNA and the programming of early embryo development.", "content": "A significant feature of the early development of fertilized echinoderm and amphibian eggs and germinating seed embryos is the utilization of genetic information that has been previously transcribed during oogenesis and seed ripening. When RNA synthesis is suppressed in the early developing embryos by actinomycin D, cordycepin, or alpha-amanitin, there is no effect on the translation of the \"preformed mRNA\", only a limited number have been thus far identified; microtubule and histone proteins in the fertilized sea urchin egg and carboxypeptidase and isocritric lyase in germinating cottonseed. Data obtained on the protein synthetic pattern at different times after the onset of development suggest that preformed mRNAs are made available to the translational system in a gradual process, thereby providing a molecular basis for the regulation of development. The possibility is considered that polyadenylation of mRNA, a reaction known to occur early after sea urchin fertilization, is responsible for regulating the release of preformed mRNA. It is shown that this reaction (polyadenylation) can be completely suppressed with little effect on the function of preformed mRNA. Finally, it is suggested, at least for the seed embryo system, that the formation of ATP may be a prerequisite for the activation of protein synthesis.", "contents": "Preformed mRNA and the programming of early embryo development. A significant feature of the early development of fertilized echinoderm and amphibian eggs and germinating seed embryos is the utilization of genetic information that has been previously transcribed during oogenesis and seed ripening. When RNA synthesis is suppressed in the early developing embryos by actinomycin D, cordycepin, or alpha-amanitin, there is no effect on the translation of the \"preformed mRNA\", only a limited number have been thus far identified; microtubule and histone proteins in the fertilized sea urchin egg and carboxypeptidase and isocritric lyase in germinating cottonseed. Data obtained on the protein synthetic pattern at different times after the onset of development suggest that preformed mRNAs are made available to the translational system in a gradual process, thereby providing a molecular basis for the regulation of development. The possibility is considered that polyadenylation of mRNA, a reaction known to occur early after sea urchin fertilization, is responsible for regulating the release of preformed mRNA. It is shown that this reaction (polyadenylation) can be completely suppressed with little effect on the function of preformed mRNA. Finally, it is suggested, at least for the seed embryo system, that the formation of ATP may be a prerequisite for the activation of protein synthesis."}
{"id": "PMID:1081827", "title": "The \"angina-producing\" myocardial segment: An approach to the interpretation of results of coronary bypass surgery.", "content": "The first 153 cases of saphenous vein aortocoronary bypass surgery performed at The Johns Hopkins Hospital were reviewed. Eighty-eight percent of the 140 late survivors reported significant symptomatic improvement. Seventy-one unselected patients consented to complete reevaluation at a mean interval of 6.1 months postoperatively. Vein bypass patency in this group was 66 percent. Eighty-two percent of these 71 patients had improved performance on electrocardiographic stress testing. There were no significant differences between hemodynamic status on pre- and postoperative studies regardless of the status of vein bypass patency. Repeat coronary angiography revealed a 30 percent incidence of new total occlusions of the intrinsic coronary circulation. Segmental wall motion on ventriculography was improved in 12 percent, unchanged in 50 percent and decreased in 38 percent of the segments analyzed. Localized electrocardiographic changes compatible with myocardial damage developed in the immediate postoperative period in 45 of these patients (63 percent); in 38 of the 45, these changes corresponded to new angiographic abnormalities. Physiologic mechanisms underlying symptomatic improvement were sought by identification of an \"angina-producing\" myocardial segment. In most instances, alleviation of angina could be related to either: (1) a patent bypass graft into an unchanged intrinsic coronary artery with presumed increased blood flow to the distal coronary segment, or (2) occlusion of the bypass graft and the corresponding coronary artery with probable infarction of previously ischemic myocardium. Current criteria for the selection of patients undergoing coronary bypass surgery are reviewed in the light of these findings. It is concluded that relief of disabling angina is the major indication for surgery. Prevention of myocardial infarction and improvement in left ventricular function are at present less reliable objectives.", "contents": "The \"angina-producing\" myocardial segment: An approach to the interpretation of results of coronary bypass surgery. The first 153 cases of saphenous vein aortocoronary bypass surgery performed at The Johns Hopkins Hospital were reviewed. Eighty-eight percent of the 140 late survivors reported significant symptomatic improvement. Seventy-one unselected patients consented to complete reevaluation at a mean interval of 6.1 months postoperatively. Vein bypass patency in this group was 66 percent. Eighty-two percent of these 71 patients had improved performance on electrocardiographic stress testing. There were no significant differences between hemodynamic status on pre- and postoperative studies regardless of the status of vein bypass patency. Repeat coronary angiography revealed a 30 percent incidence of new total occlusions of the intrinsic coronary circulation. Segmental wall motion on ventriculography was improved in 12 percent, unchanged in 50 percent and decreased in 38 percent of the segments analyzed. Localized electrocardiographic changes compatible with myocardial damage developed in the immediate postoperative period in 45 of these patients (63 percent); in 38 of the 45, these changes corresponded to new angiographic abnormalities. Physiologic mechanisms underlying symptomatic improvement were sought by identification of an \"angina-producing\" myocardial segment. In most instances, alleviation of angina could be related to either: (1) a patent bypass graft into an unchanged intrinsic coronary artery with presumed increased blood flow to the distal coronary segment, or (2) occlusion of the bypass graft and the corresponding coronary artery with probable infarction of previously ischemic myocardium. Current criteria for the selection of patients undergoing coronary bypass surgery are reviewed in the light of these findings. It is concluded that relief of disabling angina is the major indication for surgery. Prevention of myocardial infarction and improvement in left ventricular function are at present less reliable objectives."}
{"id": "PMID:1081828", "title": "Saphenous vein bypass grafts: Long-term patency and effect on the native coronary circulation.", "content": "The long-term durability of saphenous vein bypass grafts and their effect on existing intrinsic coronary artery disease remain ill defined. Therefore, sequential catheterization studies were performed in patients selected for study solely on the basis of documentation of a patent graft at an earlier study performed three to nine months postoperatively; at that time 29 patent grafts were demonstrated in 20 patients. Fifteen to 36 months postoperatively (average 22 months), 27 grafts were unchanged, 1 manifested minimal luminal irregularities and 1 was occluded. In one additional patient, studied 4 months and 4 1/2 years postoperatively, the graft was widely patent and had good distal runoff at the second study. Sequential coronary arteriograms revealed that progression of disease to complete occlusion occurred in 24 percent of vessels with severe lesions proximal to a patent graft, whereas progression of disease distal to a graft anastomosis was uncommon. Of 25 vessels not receiving grafts, disease progressed in 5 (20 percent). Grafts that are patent 3 months after operation appear to remain patent for at least 2 to 3 years, and their presence does not unduly accelerate the disease process involving the native coronary arteries.", "contents": "Saphenous vein bypass grafts: Long-term patency and effect on the native coronary circulation. The long-term durability of saphenous vein bypass grafts and their effect on existing intrinsic coronary artery disease remain ill defined. Therefore, sequential catheterization studies were performed in patients selected for study solely on the basis of documentation of a patent graft at an earlier study performed three to nine months postoperatively; at that time 29 patent grafts were demonstrated in 20 patients. Fifteen to 36 months postoperatively (average 22 months), 27 grafts were unchanged, 1 manifested minimal luminal irregularities and 1 was occluded. In one additional patient, studied 4 months and 4 1/2 years postoperatively, the graft was widely patent and had good distal runoff at the second study. Sequential coronary arteriograms revealed that progression of disease to complete occlusion occurred in 24 percent of vessels with severe lesions proximal to a patent graft, whereas progression of disease distal to a graft anastomosis was uncommon. Of 25 vessels not receiving grafts, disease progressed in 5 (20 percent). Grafts that are patent 3 months after operation appear to remain patent for at least 2 to 3 years, and their presence does not unduly accelerate the disease process involving the native coronary arteries."}
{"id": "PMID:1081829", "title": "Changes in native coronary arteries after coronary bypass surgery. Role of graft patency, serum lipids and hypertension.", "content": "Sixty-seven patients were studied by coronary angiography early (mean 3 weeks) and late (mean 13 months) after coronary bypass surgery to assess changes in the native coronary vessels. Among the 208 nongrafted arteries progression of disease was found in 2.9 percent. In arteries that were normal before operation, the rate was 0.7 percent; in those with luminal obstructions the rate was 7.6 percent (P less than 0.05). Progression of disease occurred in 6 of the 67 patients (8.9 percent). In five bypassed arteries (5 percent), progression of disease occurred at or near the anastomotic site; in this subset the procedure was classified a technical failure. Progression of disease distal to graft insertion occurred in 2.4 percent of cases. The greatest incidence of progression took place proximal to graft insertion, in 24.2 percent of the grafted arteries. This rate differed significantly from the rate in non-grafted arteries (P less than 0.001) and in distal segments of grafted arteries (P less than 0.001). If the grafts were patent in the late control study, the progression of disease proximally occurred at a rate of 24 percent; if they were occluded, the rate was 25 percent. The data on timing of graft occlusion suggested that graft patency was related to the proximal progression. The prevalence of hyperlipidemia or hypertension did not correlate with progression of disease in any group.", "contents": "Changes in native coronary arteries after coronary bypass surgery. Role of graft patency, serum lipids and hypertension. Sixty-seven patients were studied by coronary angiography early (mean 3 weeks) and late (mean 13 months) after coronary bypass surgery to assess changes in the native coronary vessels. Among the 208 nongrafted arteries progression of disease was found in 2.9 percent. In arteries that were normal before operation, the rate was 0.7 percent; in those with luminal obstructions the rate was 7.6 percent (P less than 0.05). Progression of disease occurred in 6 of the 67 patients (8.9 percent). In five bypassed arteries (5 percent), progression of disease occurred at or near the anastomotic site; in this subset the procedure was classified a technical failure. Progression of disease distal to graft insertion occurred in 2.4 percent of cases. The greatest incidence of progression took place proximal to graft insertion, in 24.2 percent of the grafted arteries. This rate differed significantly from the rate in non-grafted arteries (P less than 0.001) and in distal segments of grafted arteries (P less than 0.001). If the grafts were patent in the late control study, the progression of disease proximally occurred at a rate of 24 percent; if they were occluded, the rate was 25 percent. The data on timing of graft occlusion suggested that graft patency was related to the proximal progression. The prevalence of hyperlipidemia or hypertension did not correlate with progression of disease in any group."}
{"id": "PMID:1081830", "title": "Heterografts as aortocoronary bypass conduits in human beings.", "content": "Clinical and necropsy observations are described in a man in whom two sheep carotid arterial heterografts were inserted as aortocoronary bypass conduits 30 months before death, and in whom a canine saphenous vein heterograft was inserted several hours before death. All three grafts failed. The lumens of the sheep carotid arterial heterografts had closed by the time of catheterization 6 months after operation; at necropsy both grafts were totally occluded at their coronary and aortic ends and their media and adventitia were infiltrated by granulomatous inflammatory cells. Little information is available regarding the use of heterografts as coronary bypass conduits. Examination of previous reports describing heterografts for vascular reconstruction or bypass in both man and experimental animals and observations in our patient suggest that heterografts are unsatisfactory as aortocoronary bypass conduits.", "contents": "Heterografts as aortocoronary bypass conduits in human beings. Clinical and necropsy observations are described in a man in whom two sheep carotid arterial heterografts were inserted as aortocoronary bypass conduits 30 months before death, and in whom a canine saphenous vein heterograft was inserted several hours before death. All three grafts failed. The lumens of the sheep carotid arterial heterografts had closed by the time of catheterization 6 months after operation; at necropsy both grafts were totally occluded at their coronary and aortic ends and their media and adventitia were infiltrated by granulomatous inflammatory cells. Little information is available regarding the use of heterografts as coronary bypass conduits. Examination of previous reports describing heterografts for vascular reconstruction or bypass in both man and experimental animals and observations in our patient suggest that heterografts are unsatisfactory as aortocoronary bypass conduits."}
{"id": "PMID:1081832", "title": "Disappearance of abnormal Q waves after aortocoronary bypass surgery.", "content": "Sixty-one patients were selected from 100 consecutive patients under-going aortocoronary artery bypass. The number of vessels diseased as defined by coronary arteriography and the number of bypass grafts were recorded. Review of the preoperative electrocardiograms showed an infarct pattern in 26 of the 61 patients and analysis of the postoperative electrocardiograms revealed loss of abnormal Q waves in 3 of the 26. The pre- and postoperative clinical course of these three patients is analyzed and the extent of their coronary artery disease and number of bypass grafts compared with those of the 23 patients who had persistence of the infarction pattern and the 17 patients who manifested new Q waves. Possible explanations for the disappearance of abnormal Q waves are discussed.", "contents": "Disappearance of abnormal Q waves after aortocoronary bypass surgery. Sixty-one patients were selected from 100 consecutive patients under-going aortocoronary artery bypass. The number of vessels diseased as defined by coronary arteriography and the number of bypass grafts were recorded. Review of the preoperative electrocardiograms showed an infarct pattern in 26 of the 61 patients and analysis of the postoperative electrocardiograms revealed loss of abnormal Q waves in 3 of the 26. The pre- and postoperative clinical course of these three patients is analyzed and the extent of their coronary artery disease and number of bypass grafts compared with those of the 23 patients who had persistence of the infarction pattern and the 17 patients who manifested new Q waves. Possible explanations for the disappearance of abnormal Q waves are discussed."}
{"id": "PMID:1081834", "title": "Massive gastric hemorrhage from submucosal arterial malformation.", "content": "In a patient with massive gastric bleeding, the cause was found to be rupture of a submucosal arterial malformation. The bleeding site was identified endoscopically and the lesion was removed by local resection. This is the 28th case of this entity to be reported.", "contents": "Massive gastric hemorrhage from submucosal arterial malformation. In a patient with massive gastric bleeding, the cause was found to be rupture of a submucosal arterial malformation. The bleeding site was identified endoscopically and the lesion was removed by local resection. This is the 28th case of this entity to be reported."}
{"id": "PMID:1081836", "title": "The use of soft bandage lenses in corneal disease.", "content": "Forty-one patients were treated with soft bandage lenses for various types of corneal pathology. These patients have been monitored over a two-year period. Comfort was consistently achieved with cases of bullous and exposure keratopathies. It was found that the Warner-Lambert Softcon lens gave better protection in patients with VII nerve palsies than the Bausch & Lomb SOFLENS contact lens. However, little difference was noted between the two types of lenses when treating other corneal diseases. It was also found that topical medications, with the exception of steroids, caused no complications.", "contents": "The use of soft bandage lenses in corneal disease. Forty-one patients were treated with soft bandage lenses for various types of corneal pathology. These patients have been monitored over a two-year period. Comfort was consistently achieved with cases of bullous and exposure keratopathies. It was found that the Warner-Lambert Softcon lens gave better protection in patients with VII nerve palsies than the Bausch & Lomb SOFLENS contact lens. However, little difference was noted between the two types of lenses when treating other corneal diseases. It was also found that topical medications, with the exception of steroids, caused no complications."}
{"id": "PMID:1081837", "title": "Influence of ammonium ions on mechanical and electrophysiological responses of skeletal muscle.", "content": "Substitution of ammonium ions for sodium ion initially potentiated and then depressed twitch tension of the frog sartorius muscle. The extent of potentiation and the rate of depression of the twitch were dependent upon the concentration of ammonium ions. Ammonium ion depolarized individual muscle fibers in proportion to concentration. Maximum depolarization (33 mV) occurred in muscles equilibrated in 120 mM ammonium-Ringer for 30 min. At the higher concentration of ammonium (72--120 mM), the potentiation was quickly reversed and the tension response was eliminated completely. The gradual loss of twitch tension was accompanied by progressive decrease in the electrical excitability of individual muscle fibers. Raising the extracellular calcium concentration fivefold reduced the twitch-depressant effect of ammonium ions by maintaining excitability of the muscle fibers. Caffeine contractures (3 and 10 mM) in muscles preequilibrated in 72 mM ammonium-Ringer developed similar tensions to paired controls muscles, but the onset of tension was more rapid in the ammonium-treated muscle. It was concluded that the depression of tension could be accounted for by the loss of membrane excitability and that the evidence did not support the hypothesis that ammonium ions acted at other sites in the excitation contraction coupling.", "contents": "Influence of ammonium ions on mechanical and electrophysiological responses of skeletal muscle. Substitution of ammonium ions for sodium ion initially potentiated and then depressed twitch tension of the frog sartorius muscle. The extent of potentiation and the rate of depression of the twitch were dependent upon the concentration of ammonium ions. Ammonium ion depolarized individual muscle fibers in proportion to concentration. Maximum depolarization (33 mV) occurred in muscles equilibrated in 120 mM ammonium-Ringer for 30 min. At the higher concentration of ammonium (72--120 mM), the potentiation was quickly reversed and the tension response was eliminated completely. The gradual loss of twitch tension was accompanied by progressive decrease in the electrical excitability of individual muscle fibers. Raising the extracellular calcium concentration fivefold reduced the twitch-depressant effect of ammonium ions by maintaining excitability of the muscle fibers. Caffeine contractures (3 and 10 mM) in muscles preequilibrated in 72 mM ammonium-Ringer developed similar tensions to paired controls muscles, but the onset of tension was more rapid in the ammonium-treated muscle. It was concluded that the depression of tension could be accounted for by the loss of membrane excitability and that the evidence did not support the hypothesis that ammonium ions acted at other sites in the excitation contraction coupling."}
{"id": "PMID:1081838", "title": "Non-Poisson distribution in time of small- and large-mode miniature end-plate potentials.", "content": "Minature end-plate potentials (MEPP's) were recorded from frog (Rana pipiens) sartorius muscle cells of 60--80 mum diam; MEPP frequency remained constant up to 3 h and was 100 times greater in 80-mum cells than in 6-mum cells. Rapid cooling or heating of preparations initiated or accentuated bursts of MEPP's. Records from cells under control conditions and from cells where the MEPP population had been reduced to the small mode showed many instances of small clusters of MEPP's occurring within 100-ms intervals, indicating quantal release interdependencies. Some small cells initially generated many large MEPP's 2 or more times the average amplitude and fewer small clusters. Analysis of the occurrence of both large- and small-mode MEPP's for both 1.0-s and 100-ms nonoverlapping bins demonstrated a highly significant deviation from a Poisson distribution with significance increasing with decreasing cell diameter and/or MEPP frequency. It is suggested that doublet MEPP's are generated from one release site and that large bursts results from the activity of several release sites, since the size of bursts decreases with cell diameter (junctional area). It is concluded that release characteristics are the same for large-mode (classical) and small-mode MEPP's and that the occurrence of MEPP's is not Poisson.", "contents": "Non-Poisson distribution in time of small- and large-mode miniature end-plate potentials. Minature end-plate potentials (MEPP's) were recorded from frog (Rana pipiens) sartorius muscle cells of 60--80 mum diam; MEPP frequency remained constant up to 3 h and was 100 times greater in 80-mum cells than in 6-mum cells. Rapid cooling or heating of preparations initiated or accentuated bursts of MEPP's. Records from cells under control conditions and from cells where the MEPP population had been reduced to the small mode showed many instances of small clusters of MEPP's occurring within 100-ms intervals, indicating quantal release interdependencies. Some small cells initially generated many large MEPP's 2 or more times the average amplitude and fewer small clusters. Analysis of the occurrence of both large- and small-mode MEPP's for both 1.0-s and 100-ms nonoverlapping bins demonstrated a highly significant deviation from a Poisson distribution with significance increasing with decreasing cell diameter and/or MEPP frequency. It is suggested that doublet MEPP's are generated from one release site and that large bursts results from the activity of several release sites, since the size of bursts decreases with cell diameter (junctional area). It is concluded that release characteristics are the same for large-mode (classical) and small-mode MEPP's and that the occurrence of MEPP's is not Poisson."}
{"id": "PMID:1081839", "title": "Selective arterial embolization for control of massive upper gastrointestinal bleeding.", "content": "Massive upper gastrointestinal bleeding was controlled in 11 of 15 patients by the use of selective injected arterial emboli. Embolization is most successful in the treatment of patients with demonstrated arterial bleeding sites at angiography. This group of patients generally has ulcers and it is this group in whom vasopressin infusion has the lowest success rate. At the same time we were successful in controlling only 1 of 4 patients who were bleeding from diffuse hemorrhagic gastritis, those patients in whom vasopressin infusion is very successfu, We, therefore, now embolize only patients in whom arterial bleeding sites are demonstrated at angiography or in whom vasopressin infusion has failed to control the bleeding from hemorrhagic gastritis. Our experience also indicates that short acting occlusive agents, such as autogenous blood clot mixed with aminocaproic acid, are as successful in controlling bleeding as the more permanent types of embolic material.", "contents": "Selective arterial embolization for control of massive upper gastrointestinal bleeding. Massive upper gastrointestinal bleeding was controlled in 11 of 15 patients by the use of selective injected arterial emboli. Embolization is most successful in the treatment of patients with demonstrated arterial bleeding sites at angiography. This group of patients generally has ulcers and it is this group in whom vasopressin infusion has the lowest success rate. At the same time we were successful in controlling only 1 of 4 patients who were bleeding from diffuse hemorrhagic gastritis, those patients in whom vasopressin infusion is very successfu, We, therefore, now embolize only patients in whom arterial bleeding sites are demonstrated at angiography or in whom vasopressin infusion has failed to control the bleeding from hemorrhagic gastritis. Our experience also indicates that short acting occlusive agents, such as autogenous blood clot mixed with aminocaproic acid, are as successful in controlling bleeding as the more permanent types of embolic material."}
{"id": "PMID:1081840", "title": "Transjugular approach to the liver, biliary system, and portal circulation.", "content": "More than 5 years of clinical and animal experience with the use of a transjugular approach to the liver, biliary system, and portal circulation is reviewed. It has been of clinical value in connection with hepatic manometry and venography, liver biopsy, and cholangiography. Satisfactory hepatic manometry and venography were achieved by the transjugular approach in all 47 cases where attempted; diagnostic liver specimens were obtained in 71 of 83 patients (86%); and cholangiography was successful in 48 of 52 patients with enlarged intrahepatic ducts (92%). No complications occurred with these studies. In animals, transjugular catheterization was used as a means for portal, mesenteric, and pancreatic venography. These procedures are ready for diagnostic clinical use. Therapeutic techniques explored in animals include intravascular tamponade of gastric coronary vein and the nonsurgical creation of intrahepatic portacaval shunts, both of promise in the future management of massive gastrointestinal bleeding from varices.", "contents": "Transjugular approach to the liver, biliary system, and portal circulation. More than 5 years of clinical and animal experience with the use of a transjugular approach to the liver, biliary system, and portal circulation is reviewed. It has been of clinical value in connection with hepatic manometry and venography, liver biopsy, and cholangiography. Satisfactory hepatic manometry and venography were achieved by the transjugular approach in all 47 cases where attempted; diagnostic liver specimens were obtained in 71 of 83 patients (86%); and cholangiography was successful in 48 of 52 patients with enlarged intrahepatic ducts (92%). No complications occurred with these studies. In animals, transjugular catheterization was used as a means for portal, mesenteric, and pancreatic venography. These procedures are ready for diagnostic clinical use. Therapeutic techniques explored in animals include intravascular tamponade of gastric coronary vein and the nonsurgical creation of intrahepatic portacaval shunts, both of promise in the future management of massive gastrointestinal bleeding from varices."}
{"id": "PMID:1081841", "title": "Polyvinyl alcohol (Ivalon)--a new embolic material.", "content": "Successful nonsurgical treatment of gastrointestinal bleeding and arteriovenous malformations by embolization techniques has been previously documented. 1) Compressed Ivalon sponge was found to be a suitable embolic material in animals and in four patients. 2) The material has been extensively used in surgery, and its biocompatibility has been proved. 3) Expansion of the compressed sponge to its original size after embolization makes this material extremely effective. Recanalization did not occur in animals and humans. 4) For the occlusion of larger arteries, Ivalon can be wrapped around the guidewire. Ivalon sponge absorbs blood and serum, unwraps itself allowing withdrawal of the guidewire. 5) Embolization procedures are not without risk since reflux of embolic material may occur. Therefore, these procedures have to be carried out under fluoroscopic control.", "contents": "Polyvinyl alcohol (Ivalon)--a new embolic material. Successful nonsurgical treatment of gastrointestinal bleeding and arteriovenous malformations by embolization techniques has been previously documented. 1) Compressed Ivalon sponge was found to be a suitable embolic material in animals and in four patients. 2) The material has been extensively used in surgery, and its biocompatibility has been proved. 3) Expansion of the compressed sponge to its original size after embolization makes this material extremely effective. Recanalization did not occur in animals and humans. 4) For the occlusion of larger arteries, Ivalon can be wrapped around the guidewire. Ivalon sponge absorbs blood and serum, unwraps itself allowing withdrawal of the guidewire. 5) Embolization procedures are not without risk since reflux of embolic material may occur. Therefore, these procedures have to be carried out under fluoroscopic control."}
{"id": "PMID:1081842", "title": "Complications of small dose prophylactic heparinization.", "content": "The observations reported herein on the use of prophylactic small dose heparinization are in no way intended to diminish its value but are reported and reviewed so that certain precautions may be exercised in its use. In fact, the apparent difference in the incidence of pulmonary emboli in the controls and heparinized patients may lend credence to its value. The large controlled series now being studied at various medical centers will help to confirm or deny the value of this factor in the prevention of emboli. Careful preoperative scrutiny of the patient and meticulous intraoperative hemostasis remain important factors when a patient is receiving small dose heparin therapy. Other factors such as concomitant medications, weight of the patient, frequency and technic of administration and dosage must be considered. Additionally, monitoring the hemostatic mechanism with serial hematocrit determinations and laboratory coagulation tests appears to be an important adjunct in small dose prophylactic heparinization.", "contents": "Complications of small dose prophylactic heparinization. The observations reported herein on the use of prophylactic small dose heparinization are in no way intended to diminish its value but are reported and reviewed so that certain precautions may be exercised in its use. In fact, the apparent difference in the incidence of pulmonary emboli in the controls and heparinized patients may lend credence to its value. The large controlled series now being studied at various medical centers will help to confirm or deny the value of this factor in the prevention of emboli. Careful preoperative scrutiny of the patient and meticulous intraoperative hemostasis remain important factors when a patient is receiving small dose heparin therapy. Other factors such as concomitant medications, weight of the patient, frequency and technic of administration and dosage must be considered. Additionally, monitoring the hemostatic mechanism with serial hematocrit determinations and laboratory coagulation tests appears to be an important adjunct in small dose prophylactic heparinization."}
{"id": "PMID:1081843", "title": "Combined chemotherapy and cryosurgery for oral cancer.", "content": "The inadequacies of traditional methods for control of advanced oral carcinomas at their sites of origin prompted evaluation of combined chemotherapy and cryosurgery in seventy-three patients treated since 1969. Our experience with thirty-nine unlikely candidates for salvage by other therapy is the subject of this report. The majority had recurrent disease after other therapy. The observed morbidity potential of combined chemotherapy and cryosurgery with earlier experience led to abbreviations and refinements of method that are described and consist mainly of the following. (1) A two day postcryosurgical infusion (intra-arterial) of 5-fluorouracil (1 gm per twenty-four hours, or less) in lieu of methotrexate, the systemic toxicity and therapeutic efficacy of which seem less predictable with cryosurgery. (2) Electrosurgical subtotal tumor resection at the time of initial cryosurgery to reduce swelling and magnitude of in situ tissue slough. (3) Use of a flexible copper mesh cryoprobe that enhances feasibility of in-depth wide field cryosurgery. (4) Systematic use of multiple marginal wound biopsies as a principal guide to repetitive cryosurgery or other therapeutic adjunct selection. A special warning that available toxicologic data for independent drug therapy may not be applicable in patients after cryosurgery is given. Current experience indicates that negative biopsy after such combined therapy may be 85 per cent reliable in foretelling lesion outcome. Among the thirty-nine patients reported, twenty remain alive from six months to six years, only two of whom have clinically evident recurrent disease. If such could be reasonably accomplished, comparative evaluation of single methods should precede attempts to combine two or more modes of therapy. Since neither chemotherapy nor cryosurgery, as known today, can eliminate nodal metastases, each must be regarded as potentially adjunctive to other methods for achieving the ultimate goal of a cancer-free patient. It is within this context that combined chemotherapy and cryosurgery have been applied to unfavorable candidates for cure with seemingly worthwhile gains. Potential applicability for patients with less formidable stages of disease cannot be extrapolated from this experience. Large scale controlled clinical trials must provide the ultimately conclusive test of efficacy for such combined forms of therapy before decisive revision of traditional standards of practice might result.", "contents": "Combined chemotherapy and cryosurgery for oral cancer. The inadequacies of traditional methods for control of advanced oral carcinomas at their sites of origin prompted evaluation of combined chemotherapy and cryosurgery in seventy-three patients treated since 1969. Our experience with thirty-nine unlikely candidates for salvage by other therapy is the subject of this report. The majority had recurrent disease after other therapy. The observed morbidity potential of combined chemotherapy and cryosurgery with earlier experience led to abbreviations and refinements of method that are described and consist mainly of the following. (1) A two day postcryosurgical infusion (intra-arterial) of 5-fluorouracil (1 gm per twenty-four hours, or less) in lieu of methotrexate, the systemic toxicity and therapeutic efficacy of which seem less predictable with cryosurgery. (2) Electrosurgical subtotal tumor resection at the time of initial cryosurgery to reduce swelling and magnitude of in situ tissue slough. (3) Use of a flexible copper mesh cryoprobe that enhances feasibility of in-depth wide field cryosurgery. (4) Systematic use of multiple marginal wound biopsies as a principal guide to repetitive cryosurgery or other therapeutic adjunct selection. A special warning that available toxicologic data for independent drug therapy may not be applicable in patients after cryosurgery is given. Current experience indicates that negative biopsy after such combined therapy may be 85 per cent reliable in foretelling lesion outcome. Among the thirty-nine patients reported, twenty remain alive from six months to six years, only two of whom have clinically evident recurrent disease. If such could be reasonably accomplished, comparative evaluation of single methods should precede attempts to combine two or more modes of therapy. Since neither chemotherapy nor cryosurgery, as known today, can eliminate nodal metastases, each must be regarded as potentially adjunctive to other methods for achieving the ultimate goal of a cancer-free patient. It is within this context that combined chemotherapy and cryosurgery have been applied to unfavorable candidates for cure with seemingly worthwhile gains. Potential applicability for patients with less formidable stages of disease cannot be extrapolated from this experience. Large scale controlled clinical trials must provide the ultimately conclusive test of efficacy for such combined forms of therapy before decisive revision of traditional standards of practice might result."}
{"id": "PMID:1081845", "title": "A new method for determining the blood quantity in the eye in a unit of time.", "content": "The principles of rheography with \"infinite time constant\" are utilized. After applying a suction cup electrode mechanism, by gradual increases of the vacuum, ocular pressure rises above venous pressure, and without modification of arterial inflow, the venous outflow is blocked; in consequence an increase of blood accumulation in the interior of the eye occurs. The electrical impedance variation, as a result of the latter, gives the recorded tracing a \"slow\" rising curve, that maintains the same pulse morphology. After being analyzed and elaborated on with certain mathematical formulae, this curve makes it possible to calculate the blood flow in the eye in a unit of time. Statistics on normal patients of various ages are reported.", "contents": "A new method for determining the blood quantity in the eye in a unit of time. The principles of rheography with \"infinite time constant\" are utilized. After applying a suction cup electrode mechanism, by gradual increases of the vacuum, ocular pressure rises above venous pressure, and without modification of arterial inflow, the venous outflow is blocked; in consequence an increase of blood accumulation in the interior of the eye occurs. The electrical impedance variation, as a result of the latter, gives the recorded tracing a \"slow\" rising curve, that maintains the same pulse morphology. After being analyzed and elaborated on with certain mathematical formulae, this curve makes it possible to calculate the blood flow in the eye in a unit of time. Statistics on normal patients of various ages are reported."}
{"id": "PMID:1081846", "title": "The pattern evoked responses (VER) in optic neuritis.", "content": "Five patients presenting clinically accepted signs of optic neuritis served as subjects for a study on EP's (evoked potentials); the patients were tested during the acute phase, the chronic phase and at a stage when no impairment was clinically observed. At the acute phase of optic neuritis the VER was massively altered; in the chronic phase delayed latencies depending upon the impairment of visual acuity and visual fields could be recorded. At the recovery stage, while visual acuity and visual field showed no deficit, not only delayed latencies but also reduction in the amplitude at all stimulation frequencies were recorded.", "contents": "The pattern evoked responses (VER) in optic neuritis. Five patients presenting clinically accepted signs of optic neuritis served as subjects for a study on EP's (evoked potentials); the patients were tested during the acute phase, the chronic phase and at a stage when no impairment was clinically observed. At the acute phase of optic neuritis the VER was massively altered; in the chronic phase delayed latencies depending upon the impairment of visual acuity and visual fields could be recorded. At the recovery stage, while visual acuity and visual field showed no deficit, not only delayed latencies but also reduction in the amplitude at all stimulation frequencies were recorded."}
{"id": "PMID:1081847", "title": "An automatic perimeter for glaucoma visual field screening and control. Construction and clinical cases.", "content": "An apparatus for static perimetry of the central visual field at 64 points is described. The testing is fully automatic: the test logic is programmed into a mini-computer and the results are plotted as a diagram in polar coordinates. A few cases with small or medium sized visual field defects have been analyzed by conventional methods and by the automatic instrument with satisfying results.", "contents": "An automatic perimeter for glaucoma visual field screening and control. Construction and clinical cases. An apparatus for static perimetry of the central visual field at 64 points is described. The testing is fully automatic: the test logic is programmed into a mini-computer and the results are plotted as a diagram in polar coordinates. A few cases with small or medium sized visual field defects have been analyzed by conventional methods and by the automatic instrument with satisfying results."}
{"id": "PMID:1081848", "title": "Provoked iris ischaemia in the rabbit. II. Histochemical localization of sodium fluorescein.", "content": "The distribution of injected sodium fluorescein in the iris and ciliary body of albino rabbits was studied in normal animals and in animals subjected to different surgical procedures. The study was performed with two techniques: whole-mount preparations and the paraffin-section method on freeze-dried eyes. Fluorescein distribution showed marked changes after surgical procedures as a result of reduced blood perfusion. In normal iris vessels no penetration of fluorescein was observed. The permeability barrier was impaired if there were ischaemic conditions in the anterior segment. The results were compared with clinical and iris angiographic studies.", "contents": "Provoked iris ischaemia in the rabbit. II. Histochemical localization of sodium fluorescein. The distribution of injected sodium fluorescein in the iris and ciliary body of albino rabbits was studied in normal animals and in animals subjected to different surgical procedures. The study was performed with two techniques: whole-mount preparations and the paraffin-section method on freeze-dried eyes. Fluorescein distribution showed marked changes after surgical procedures as a result of reduced blood perfusion. In normal iris vessels no penetration of fluorescein was observed. The permeability barrier was impaired if there were ischaemic conditions in the anterior segment. The results were compared with clinical and iris angiographic studies."}
{"id": "PMID:1081849", "title": "Fundus drawings versus photography.", "content": "Under adverse optical conditions (turbidity of the media) a well-trained observer/artist, using slit-lamp stereomicroscopy, is able to display in a drawing considerably more correct information about a fundus than what a fundus camera records. To a lesser degree this holds under favorable conditions too. General advocation of photography for being more objective is questioned; even if true, meme objectivity, i.e. no false information, would not make up for loss of important details. This paper intends to prove these points experimentally on an object (a nummulite shell) unfamiliar to the artist charged to do the drawing. The latter and photographs taken under varying conditions were compared inter se and with a high resolution scanning electron micrograph.", "contents": "Fundus drawings versus photography. Under adverse optical conditions (turbidity of the media) a well-trained observer/artist, using slit-lamp stereomicroscopy, is able to display in a drawing considerably more correct information about a fundus than what a fundus camera records. To a lesser degree this holds under favorable conditions too. General advocation of photography for being more objective is questioned; even if true, meme objectivity, i.e. no false information, would not make up for loss of important details. This paper intends to prove these points experimentally on an object (a nummulite shell) unfamiliar to the artist charged to do the drawing. The latter and photographs taken under varying conditions were compared inter se and with a high resolution scanning electron micrograph."}
{"id": "PMID:1081850", "title": "[The alteration of the human ERG in light- and darkadapted state under normal and increased intraocular pressure (author's transl)].", "content": "Electroretinograms of 19 human eyes in light- and darkadapted state under normal and increased intraocular pressure (increased by a spring pressure dynamometer) were recorded (Pm = (Ps + Pd)/2). On physiological intraocular pressure the a-wave showed a low increase in amplitude after 25 min of dark adaptation, the b-wave a great one. After increasing the intraocular pressure no significant influence of the state of adaptation to the magnitude of the a- and b-wave could be found.", "contents": "[The alteration of the human ERG in light- and darkadapted state under normal and increased intraocular pressure (author's transl)]. Electroretinograms of 19 human eyes in light- and darkadapted state under normal and increased intraocular pressure (increased by a spring pressure dynamometer) were recorded (Pm = (Ps + Pd)/2). On physiological intraocular pressure the a-wave showed a low increase in amplitude after 25 min of dark adaptation, the b-wave a great one. After increasing the intraocular pressure no significant influence of the state of adaptation to the magnitude of the a- and b-wave could be found."}
{"id": "PMID:1081851", "title": "Vitrectomy in exogenous Candida endophthalmitis.", "content": "A case of Candida endophthalmitis was clinically diagnosed three weeks after perforating injury. It was successfully treated with vitrectomy and intravitreal injection of 5 mcg of amphotericin B. A Candida speices was cultured from the vitreous aspirations.", "contents": "Vitrectomy in exogenous Candida endophthalmitis. A case of Candida endophthalmitis was clinically diagnosed three weeks after perforating injury. It was successfully treated with vitrectomy and intravitreal injection of 5 mcg of amphotericin B. A Candida speices was cultured from the vitreous aspirations."}
{"id": "PMID:1081852", "title": "Hypersensitivity towards alpha-crystalline in the heterochromia syndrome.", "content": "In 4 cases of heterochromic cyclitis with cataract, 5 cases of herpetic iridocyclitis, and 5 cases of senile cataract, hypersensitivity to both alpha-crystalline and uveal pigment was investigated by lymphocyte-transformation and leukocyte-migration tests. In 3 cases of heterochromic cyclitis with cataracts, cellular hypersensitivity to alpha-crystalline was proven. Basing on these findings, the role of hypersensitivity to lens proteins in the etiology of heterochromic cyclitis is suggested.", "contents": "Hypersensitivity towards alpha-crystalline in the heterochromia syndrome. In 4 cases of heterochromic cyclitis with cataract, 5 cases of herpetic iridocyclitis, and 5 cases of senile cataract, hypersensitivity to both alpha-crystalline and uveal pigment was investigated by lymphocyte-transformation and leukocyte-migration tests. In 3 cases of heterochromic cyclitis with cataracts, cellular hypersensitivity to alpha-crystalline was proven. Basing on these findings, the role of hypersensitivity to lens proteins in the etiology of heterochromic cyclitis is suggested."}
{"id": "PMID:1081853", "title": "Effects of some phlogistics on the capillary resistance of the rabbit conjunctiva.", "content": "Applying certain phlogistics (10% ol. sinapis, tinct. capsici, Dionin) and 1.5% histamine solution to the conjunctival sac of rabbits led to a mathematically significant decrease in conjunctival capillary resistance in every experimental animal. The effect occurred 20 to 30 min after application of the various pharmacons.", "contents": "Effects of some phlogistics on the capillary resistance of the rabbit conjunctiva. Applying certain phlogistics (10% ol. sinapis, tinct. capsici, Dionin) and 1.5% histamine solution to the conjunctival sac of rabbits led to a mathematically significant decrease in conjunctival capillary resistance in every experimental animal. The effect occurred 20 to 30 min after application of the various pharmacons."}
{"id": "PMID:1081854", "title": "Side-effects of local anesthetics on the corneal epithelium of the rabbit eye.", "content": "The toxic side-effects of 0.4% Novesine and Chibro-Kerakain were investigated by scanning- and transmission electron microscopy in 15 rabbit corneae each in intervals of 3, 6, 10, 15 and 60 mins. The lesions following Kerakain were larger and occurred earlier. After 60 mins, the specimens after Novesine application showed an almost complete repair process, whereas after Kerakain the epithelial surface appeared to be functionally impaired.", "contents": "Side-effects of local anesthetics on the corneal epithelium of the rabbit eye. The toxic side-effects of 0.4% Novesine and Chibro-Kerakain were investigated by scanning- and transmission electron microscopy in 15 rabbit corneae each in intervals of 3, 6, 10, 15 and 60 mins. The lesions following Kerakain were larger and occurred earlier. After 60 mins, the specimens after Novesine application showed an almost complete repair process, whereas after Kerakain the epithelial surface appeared to be functionally impaired."}
{"id": "PMID:1081855", "title": "Ultrasonic findings in the vitreous body at different ages and in patients with detachment of the retina.", "content": "The effect of age on degeneration of the vitreous body was studied in 77 patients and in 123 eyes. The patients were divided into three groups by age, with a mean age of 16, 35 and 72 years, respectively. In the two younger groups the vitreous body generally appeared to be acoustically homogeneous, while the oldest group showed heterogeneity of the vitreous body in a majority of cases. Syneresis of the vitreous body yielded a highly characteristic echogram. A total of 22 eyes with unilateral idiopathic detachment of the retina were examined, and in all of them degeneration of the vitreous body was clearly detected. Examination of the other, \"normal\" eye revealed only four eyes with an acoustically homogeneous vitreous body. Six patients had severe syneresis in bthe eyes. The evident conclusion is that detachment of the retina even in a single eye is often accompanied by degeneration of the vitreous body in the other eye as well.", "contents": "Ultrasonic findings in the vitreous body at different ages and in patients with detachment of the retina. The effect of age on degeneration of the vitreous body was studied in 77 patients and in 123 eyes. The patients were divided into three groups by age, with a mean age of 16, 35 and 72 years, respectively. In the two younger groups the vitreous body generally appeared to be acoustically homogeneous, while the oldest group showed heterogeneity of the vitreous body in a majority of cases. Syneresis of the vitreous body yielded a highly characteristic echogram. A total of 22 eyes with unilateral idiopathic detachment of the retina were examined, and in all of them degeneration of the vitreous body was clearly detected. Examination of the other, \"normal\" eye revealed only four eyes with an acoustically homogeneous vitreous body. Six patients had severe syneresis in bthe eyes. The evident conclusion is that detachment of the retina even in a single eye is often accompanied by degeneration of the vitreous body in the other eye as well."}
{"id": "PMID:1081856", "title": "[Tension under controlled conditions in the encircling operation (author's transl)].", "content": "The complications, which occur in encircling-operations, such as String-Syndrome, scleral Nekrosis, glaucoma-development (due to narrowing the anterior chamber) as well as closure of the central artery most frequently happen, when the globe has been tightened too much. From the calculations we have make using the cerclage element in reducing the circumference and volume (Fig. 2) we have shown that an identation of the sclera more than 3 mm deep is not advisable. The Silicon-Cerclage-Materials, which we use, are tested by the tension-testing machine, which determines the modulus of elasticity. The stress, that is force per sectional area, is determined in relation to the amount of elongation in the material (Fig. 3). A round silicon rod with a diameter of 2 mm and a modulus of elasticity between 150 and 200 p/mm2 proved most effective. In addition, we have conducted experiments with the Cerclage-rods on enucleated human eyes (Fig. 4). In one experiment the intraocular pressure was determined in relation to the pull of the weight upon the globe (Fig. 5). In a second experiment the reduction of the circumference was related to the height of the intraocular pressure (Fig. 6). The elastic Cerlage-rod has the advantage of creeping, when tensed, it tries to regain its original length. However, the globe, owing to the pressure within it, works against the creeping. With the help of the stress-strain diagram (Fig. 7) and the experiments on the human eye we can determine more accurately the degree of the creeping. In index 1 we can see how much the circumference has been finally reduced including the creeping in relation to the height of the intraocular pressure. The intraocular pressure should not be increased above 35 mm Hg. Otherwise: 1. the circumference of the globe will be considerably more reduced; 2. the intraocular pressure will rise much more slowly when compared to results at the lower eye-tension and 3. the volume reducing-effect will diminish markedly. It is important to measure the intraocular pressure before the end of the operation in order to avoid the above mentioned complications.", "contents": "[Tension under controlled conditions in the encircling operation (author's transl)]. The complications, which occur in encircling-operations, such as String-Syndrome, scleral Nekrosis, glaucoma-development (due to narrowing the anterior chamber) as well as closure of the central artery most frequently happen, when the globe has been tightened too much. From the calculations we have make using the cerclage element in reducing the circumference and volume (Fig. 2) we have shown that an identation of the sclera more than 3 mm deep is not advisable. The Silicon-Cerclage-Materials, which we use, are tested by the tension-testing machine, which determines the modulus of elasticity. The stress, that is force per sectional area, is determined in relation to the amount of elongation in the material (Fig. 3). A round silicon rod with a diameter of 2 mm and a modulus of elasticity between 150 and 200 p/mm2 proved most effective. In addition, we have conducted experiments with the Cerclage-rods on enucleated human eyes (Fig. 4). In one experiment the intraocular pressure was determined in relation to the pull of the weight upon the globe (Fig. 5). In a second experiment the reduction of the circumference was related to the height of the intraocular pressure (Fig. 6). The elastic Cerlage-rod has the advantage of creeping, when tensed, it tries to regain its original length. However, the globe, owing to the pressure within it, works against the creeping. With the help of the stress-strain diagram (Fig. 7) and the experiments on the human eye we can determine more accurately the degree of the creeping. In index 1 we can see how much the circumference has been finally reduced including the creeping in relation to the height of the intraocular pressure. The intraocular pressure should not be increased above 35 mm Hg. Otherwise: 1. the circumference of the globe will be considerably more reduced; 2. the intraocular pressure will rise much more slowly when compared to results at the lower eye-tension and 3. the volume reducing-effect will diminish markedly. It is important to measure the intraocular pressure before the end of the operation in order to avoid the above mentioned complications."}
{"id": "PMID:1081857", "title": "The treatment of systemic lupus erythematosus (SLE) with the T-cell immunostimulant drug levamisole: A case report.", "content": "A 23-year-old woman with a nine-month history of SLE, uncontrolled with high daily doses of steroids, was treated with the T-cell immunostimulant drug, levamisole. Following a single oral three-day course of therapy with this agent her immunologic parameters and clinical manifestations underwent marked regression. Continuous two-weekly course of this drug at three days per course have allowed rapid reduction of her steroid and resulted in complete remission of her symptoms. The theoretical considerations for the use of levamisole dovetailed perfectly with the observed response to this agent.", "contents": "The treatment of systemic lupus erythematosus (SLE) with the T-cell immunostimulant drug levamisole: A case report. A 23-year-old woman with a nine-month history of SLE, uncontrolled with high daily doses of steroids, was treated with the T-cell immunostimulant drug, levamisole. Following a single oral three-day course of therapy with this agent her immunologic parameters and clinical manifestations underwent marked regression. Continuous two-weekly course of this drug at three days per course have allowed rapid reduction of her steroid and resulted in complete remission of her symptoms. The theoretical considerations for the use of levamisole dovetailed perfectly with the observed response to this agent."}
{"id": "PMID:1081859", "title": "Local, passive transfer of purified protein derivative-delayed tuberculin hypersensitivity by guinea pig thymus-dependent lymphocytes.", "content": "Delayed skin reactions to purified protein derivative could be produced in guinea pigs by passive transfer of the population of sensitized lymphocytes, which have the ability to form rosettes with rabbit erythrocytes. Because it has been shown earlier that guinea pig thymus-dependent lymphocytes rosette with rabbit erythrocytes, it is concluded that thymus-dependent lymphocytes mediate delayed type hypersensitivity.", "contents": "Local, passive transfer of purified protein derivative-delayed tuberculin hypersensitivity by guinea pig thymus-dependent lymphocytes. Delayed skin reactions to purified protein derivative could be produced in guinea pigs by passive transfer of the population of sensitized lymphocytes, which have the ability to form rosettes with rabbit erythrocytes. Because it has been shown earlier that guinea pig thymus-dependent lymphocytes rosette with rabbit erythrocytes, it is concluded that thymus-dependent lymphocytes mediate delayed type hypersensitivity."}
{"id": "PMID:1081860", "title": "Evidence of congenitally nonfunctioning cilia in the tracheobronchial tract in two subjects.", "content": "Mucociliary transport in the tracheobronchial tract was studies in 2 subjects by having them inhale a radioactively tagged test aerosol and by taking external measurements of the radioacitivity in the lungs for 2 hours. Both subjects had living, yet immotile seprmatozoa; the sperm tails lacked normal dynein arms, which are essential for the bending movements of sperm tails as well as of other cilia. The subjects were classified as suffering from Kartagener's syndrome (situs inversus, chronic sinusitis, and bronchiectasis) and were shown to have an extremely slow, probably absent transport. The results of the study were in agreement with a recent hypothesis explaining Kartagener's syndrome as due to a genetic lack of dynein arms. An estimate of the importance of mucociliary transport as a protective mechanism of the lung may be possible by examining patients suffering from Kartagener's syndrome.", "contents": "Evidence of congenitally nonfunctioning cilia in the tracheobronchial tract in two subjects. Mucociliary transport in the tracheobronchial tract was studies in 2 subjects by having them inhale a radioactively tagged test aerosol and by taking external measurements of the radioacitivity in the lungs for 2 hours. Both subjects had living, yet immotile seprmatozoa; the sperm tails lacked normal dynein arms, which are essential for the bending movements of sperm tails as well as of other cilia. The subjects were classified as suffering from Kartagener's syndrome (situs inversus, chronic sinusitis, and bronchiectasis) and were shown to have an extremely slow, probably absent transport. The results of the study were in agreement with a recent hypothesis explaining Kartagener's syndrome as due to a genetic lack of dynein arms. An estimate of the importance of mucociliary transport as a protective mechanism of the lung may be possible by examining patients suffering from Kartagener's syndrome."}
{"id": "PMID:1081861", "title": "Intestinal bypass in morbid obesity: long-term metabolic sequelae.", "content": "A review of 51 patients undergoing jejunoileal bypass for morbid obesity is presented. There were two postoperative deaths; the 49 remaining patients have been followed for up to five years. Weight loss has been acceptable, the complications for the most part being transient and easily controlled. We believe that jejunoileal bypass for morbid obesity is a useful treatment modality in carefully selected patients.", "contents": "Intestinal bypass in morbid obesity: long-term metabolic sequelae. A review of 51 patients undergoing jejunoileal bypass for morbid obesity is presented. There were two postoperative deaths; the 49 remaining patients have been followed for up to five years. Weight loss has been acceptable, the complications for the most part being transient and easily controlled. We believe that jejunoileal bypass for morbid obesity is a useful treatment modality in carefully selected patients."}
{"id": "PMID:1081862", "title": "Smooth muscle antibody in heroin addicts.", "content": "The occurrence of autoantibodies and the concentrations of serum immunoglobulins were studied in 102 heroin addicts, 20 former addicts who had abstained from heroin for 1 year or more, and 40 normal control subjects. Antibodies to smooth muscle (46%) and lymphocytotoxic antibodies (30%) were detected in the active heroin users, and there was a significantly positive correlation between these autoantibodies. Absorption experiments strongly suggested antigenic cross-reactivity between smooth muscle and lymphocyte membrane antigens. The presence of smooth muscle antibody in addicts was not clearly correlated with signs of active liver disease. The occurrence of smooth muscle antibody (10%) and lymphocytotoxic antibodies (15%) was significantly lower in the former heroin addicts than among active drug users. A significant elevation of IgM was found in the active heroin addicts. Gamma-M globulin was lower among the former heroin addicts but still elevated when compared with normal controls.", "contents": "Smooth muscle antibody in heroin addicts. The occurrence of autoantibodies and the concentrations of serum immunoglobulins were studied in 102 heroin addicts, 20 former addicts who had abstained from heroin for 1 year or more, and 40 normal control subjects. Antibodies to smooth muscle (46%) and lymphocytotoxic antibodies (30%) were detected in the active heroin users, and there was a significantly positive correlation between these autoantibodies. Absorption experiments strongly suggested antigenic cross-reactivity between smooth muscle and lymphocyte membrane antigens. The presence of smooth muscle antibody in addicts was not clearly correlated with signs of active liver disease. The occurrence of smooth muscle antibody (10%) and lymphocytotoxic antibodies (15%) was significantly lower in the former heroin addicts than among active drug users. A significant elevation of IgM was found in the active heroin addicts. Gamma-M globulin was lower among the former heroin addicts but still elevated when compared with normal controls."}
{"id": "PMID:1081858", "title": "[Neonatal hepatitis with alpha-1-antitrypsin deficit. Apropos of a personal case].", "content": "The authors report a case of neonatal hepatitis with alpha-1-antitrypsin occuring in a child of ZZ phenotype. The anatomopathological study carried out on two liver biopsies showed changes of common cholestatic hepatitis developing into cirrhosis, as well as intrahepatocytary globulins. Moreover, these globulins, P.A.S. positive after treatment by alphaamylase, fix an antialpha-1-antitrypsine antiserum. Ultrastructural analysis shows them to be masses of amorphous material, feebly osmiophilic, outlined by a unitary membrane the moniliform aspect of which recalls the ergastoplasmic membrane. These findings are identical to those already made in cases of cirrhogenous neonatal hepatitis by alpha-1-antitrypsine deficit reported in the literature. They point out the irreversibility of the affection which, after a stage of cholestatic hepatitis with or without inflammatory portal fibrosis, develops into cirrhosis. At this stage cholestasis has regressed or disappeared whereas portal sclerosis, often infiltrated with free elements, surrounds hepatic lobules and biliary neocanaliculi. But the globulins are still present and appear to be the specific feature of this deficit. By their ultrastructural and immuno-histochemical features, these globulins would represent a form of accumulation of alpha-1-antitrypsin in the hepatocytes which normally carry out the synthesis of this antienzyme. Accumulation in the hepatocytes proves excretory disturbance of hypothetical mechanism: structural anomaly, changes  in the permeability of the membrane. Its role in the occurrence of hepatitis or cirrhosis lesions is still to be demonstrated but one may think that it consists in absence of inhibition of the enzymatic factors discharged during agressions.", "contents": "[Neonatal hepatitis with alpha-1-antitrypsin deficit. Apropos of a personal case]. The authors report a case of neonatal hepatitis with alpha-1-antitrypsin occuring in a child of ZZ phenotype. The anatomopathological study carried out on two liver biopsies showed changes of common cholestatic hepatitis developing into cirrhosis, as well as intrahepatocytary globulins. Moreover, these globulins, P.A.S. positive after treatment by alphaamylase, fix an antialpha-1-antitrypsine antiserum. Ultrastructural analysis shows them to be masses of amorphous material, feebly osmiophilic, outlined by a unitary membrane the moniliform aspect of which recalls the ergastoplasmic membrane. These findings are identical to those already made in cases of cirrhogenous neonatal hepatitis by alpha-1-antitrypsine deficit reported in the literature. They point out the irreversibility of the affection which, after a stage of cholestatic hepatitis with or without inflammatory portal fibrosis, develops into cirrhosis. At this stage cholestasis has regressed or disappeared whereas portal sclerosis, often infiltrated with free elements, surrounds hepatic lobules and biliary neocanaliculi. But the globulins are still present and appear to be the specific feature of this deficit. By their ultrastructural and immuno-histochemical features, these globulins would represent a form of accumulation of alpha-1-antitrypsin in the hepatocytes which normally carry out the synthesis of this antienzyme. Accumulation in the hepatocytes proves excretory disturbance of hypothetical mechanism: structural anomaly, changes  in the permeability of the membrane. Its role in the occurrence of hepatitis or cirrhosis lesions is still to be demonstrated but one may think that it consists in absence of inhibition of the enzymatic factors discharged during agressions."}
{"id": "PMID:1081865", "title": "Discoid lupus erythematosus as part of a larger disease spectrum. Correlation of clinical features with laboratory findings in lupus erythematosus.", "content": "This study compares the immunologic features of a homogeneous group of patients with discoid lupus erythematosus (DLE) strictly limited to the skin (group 1) with those of patients with active discoid skin lesions plus visceral involvement (group 2) and with those of lupus erythematosus (LE) patients with proliferative glomerulonephritis (group 3). Positive antinuclear antibody (ANA) was found in 4% of group 1, 93% of group 2, and 100% of group 3. Low total hemolytic complement (CH50) was found in 4% of group 1, 47% of group 2, and 100% of group 3. Antibodies to native DNA (nDNA) were not found in group 1, were rarely found in group 2, and were present in nearly all patients in group 3. No group 1 patient had subepidermal immunoglobulin deposits in normal skin, 20% of group 2 had this finding, and 100% of group 3 had this finding. The ability to develop chronic discoid skin lesions appears to be associated with a reduced incidence of immunologic parameters of disease activity. The data suggest that patients with active discoid skin lesions rarely have severe renal disease.", "contents": "Discoid lupus erythematosus as part of a larger disease spectrum. Correlation of clinical features with laboratory findings in lupus erythematosus. This study compares the immunologic features of a homogeneous group of patients with discoid lupus erythematosus (DLE) strictly limited to the skin (group 1) with those of patients with active discoid skin lesions plus visceral involvement (group 2) and with those of lupus erythematosus (LE) patients with proliferative glomerulonephritis (group 3). Positive antinuclear antibody (ANA) was found in 4% of group 1, 93% of group 2, and 100% of group 3. Low total hemolytic complement (CH50) was found in 4% of group 1, 47% of group 2, and 100% of group 3. Antibodies to native DNA (nDNA) were not found in group 1, were rarely found in group 2, and were present in nearly all patients in group 3. No group 1 patient had subepidermal immunoglobulin deposits in normal skin, 20% of group 2 had this finding, and 100% of group 3 had this finding. The ability to develop chronic discoid skin lesions appears to be associated with a reduced incidence of immunologic parameters of disease activity. The data suggest that patients with active discoid skin lesions rarely have severe renal disease."}
{"id": "PMID:1081869", "title": "Electrophysiological orthosis for the upper extremity in hemiplegia: feasibility study.", "content": "A versatile two channel stimulator with cutaneous electrodes was designed and built to test the feasibility of multi-channel upper extremity functional electrical stimulation in hemiplegic patients. Preliminary experiments led to the choice of stimulation for hand opening (extensor carpi radialis and ulnaris, extensor digitorum communis) and elbow extension (triceps). Three channel stimulation including the deltoideus muscle was attempted successfully in one patient. Five of eight patients showed substantial improvement with the orthosis and three of these five showed some therapeutic effect over a period of two months. The stimulation was proportionally controlled by the patient by means of movement of the nonaffected shoulder. Functional tasks such as the shifting of an object between two specified areas on a desk were used for evaluation of performance. Tasks that were impossible without stimulation were easily performed with stimulation and after minimal training. The results obtained were significant for the functional rehabilitation of hemiplegic subjects. It is concluded that a more extensive clinical evaluation program including regular long term training of the subjects is justified.", "contents": "Electrophysiological orthosis for the upper extremity in hemiplegia: feasibility study. A versatile two channel stimulator with cutaneous electrodes was designed and built to test the feasibility of multi-channel upper extremity functional electrical stimulation in hemiplegic patients. Preliminary experiments led to the choice of stimulation for hand opening (extensor carpi radialis and ulnaris, extensor digitorum communis) and elbow extension (triceps). Three channel stimulation including the deltoideus muscle was attempted successfully in one patient. Five of eight patients showed substantial improvement with the orthosis and three of these five showed some therapeutic effect over a period of two months. The stimulation was proportionally controlled by the patient by means of movement of the nonaffected shoulder. Functional tasks such as the shifting of an object between two specified areas on a desk were used for evaluation of performance. Tasks that were impossible without stimulation were easily performed with stimulation and after minimal training. The results obtained were significant for the functional rehabilitation of hemiplegic subjects. It is concluded that a more extensive clinical evaluation program including regular long term training of the subjects is justified."}
{"id": "PMID:1081870", "title": "Complications of ornamental Christmas bulb ingestion. Case report and review of the literature.", "content": "Unusual complications ensued when a 14-month-old boy ingested an ornamental Christmas bulb. Profuse rectal bleeding, a large ischiorectal abscess, and an acute condition of the abdomen necessiated a sigmoid colostomy with drainage of the ischiorectal abscess. Following this, repeated episodes of hemorrhage via the colostomy and rectum required multiple operative procedures. The last of these was a total colectomy with an ileostomy and rectal mucous fistula. The patient subsequently developed intestinal obstruction that required lysis of adhesions and drainage of a chronic subhepatic abscess, due to perforation of the terminal part of the ileum. The patient required five months of hospitalization and further surgery to reconstruct gastrointestinal continuity. Hemorrhage is an infrequent complication of foreign body ingestion, as reported in the literature. We theorize that the problems in this case were the result of the extreme thinness (0.033 cm) and the brittleness of the glass fragments.", "contents": "Complications of ornamental Christmas bulb ingestion. Case report and review of the literature. Unusual complications ensued when a 14-month-old boy ingested an ornamental Christmas bulb. Profuse rectal bleeding, a large ischiorectal abscess, and an acute condition of the abdomen necessiated a sigmoid colostomy with drainage of the ischiorectal abscess. Following this, repeated episodes of hemorrhage via the colostomy and rectum required multiple operative procedures. The last of these was a total colectomy with an ileostomy and rectal mucous fistula. The patient subsequently developed intestinal obstruction that required lysis of adhesions and drainage of a chronic subhepatic abscess, due to perforation of the terminal part of the ileum. The patient required five months of hospitalization and further surgery to reconstruct gastrointestinal continuity. Hemorrhage is an infrequent complication of foreign body ingestion, as reported in the literature. We theorize that the problems in this case were the result of the extreme thinness (0.033 cm) and the brittleness of the glass fragments."}
{"id": "PMID:1081871", "title": "Dorsal column stimulation. Its effect on the somatosensory evoked response.", "content": "Several authors have shown that dorsal column stimulation (DCS) modifies appreciation of pain. We studied the effects of DCS on the somatosensory evoked response (SER) in six subjects. Somatosensory evoked responses to stimulation of the tibial nerve and effects of varying frequencies and intensities of DCS on SER components were recorded. During DCS there was attenuation of late SER components with little effect on early components. Stimulus intensities greater than necessary to relieve pain suppressed all SER components. Our findings suggest that the minimal effective DCS variables to relieve pain are those that produce selective suppression of late SER components.", "contents": "Dorsal column stimulation. Its effect on the somatosensory evoked response. Several authors have shown that dorsal column stimulation (DCS) modifies appreciation of pain. We studied the effects of DCS on the somatosensory evoked response (SER) in six subjects. Somatosensory evoked responses to stimulation of the tibial nerve and effects of varying frequencies and intensities of DCS on SER components were recorded. During DCS there was attenuation of late SER components with little effect on early components. Stimulus intensities greater than necessary to relieve pain suppressed all SER components. Our findings suggest that the minimal effective DCS variables to relieve pain are those that produce selective suppression of late SER components."}
{"id": "PMID:1081867", "title": "Lung proteolytic activity and serum protease inhibition after NO2 exposure.", "content": "Proteolytic activity (PA) of lung extracts was measured in control hamsters and in those exposed to 20 ppm NO2 for 24 hours (acute) and 30 days (chronic). Total PA of these lungs as measured by pH 7.4 caseinolysis was slightly increased after the 24-hour, but normal following the 30-day exposure. Lung extract fractions from NO2-exposed animals display an increased PA at pH 3.0 and 9.0, but are near control levels at pH 7.0. Serum protease inhibitor (PI) activity is significantly increased after short NO2 exposure as compared with control conditions. No significant increase is observed in PI after chronic exposure. Increase in 24-hr NO2 lung extract PA is associated with increase in PI, which may neutralize the augmented proteolysis. These studies are consistent with previous observations that indicate prolonged NO2 exposure does not produce significant emphysema in the hamster lung.", "contents": "Lung proteolytic activity and serum protease inhibition after NO2 exposure. Proteolytic activity (PA) of lung extracts was measured in control hamsters and in those exposed to 20 ppm NO2 for 24 hours (acute) and 30 days (chronic). Total PA of these lungs as measured by pH 7.4 caseinolysis was slightly increased after the 24-hour, but normal following the 30-day exposure. Lung extract fractions from NO2-exposed animals display an increased PA at pH 3.0 and 9.0, but are near control levels at pH 7.0. Serum protease inhibitor (PI) activity is significantly increased after short NO2 exposure as compared with control conditions. No significant increase is observed in PI after chronic exposure. Increase in 24-hr NO2 lung extract PA is associated with increase in PI, which may neutralize the augmented proteolysis. These studies are consistent with previous observations that indicate prolonged NO2 exposure does not produce significant emphysema in the hamster lung."}
{"id": "PMID:1081872", "title": "Exteriorization of thoracic duct lymph. Theoretical considerations and an experimental model.", "content": "Successful manipulation of the immune response will ultimately provide the answers for controlling homograft rejection, reversing autoimmune states, and quite possibly for curing carcinoma and other malignant neoplasms. During the past decade, many new drugs and techniques have been developed that alter the immune state. The theoretical basis for approaching the immune system via the thoracic duct is based on the fact that 90% to 95% of the cells in thoracic duct lymph are thymus-derived lymphocytes. Therefore, by tapping the thoracic duct, we could conceivably manipulate the T lymphocytes and control the cell-mediated arm of the immune system. This has in fact been done in renal transplantation and autoimmune diseases. An experimental canine model was developed that exteriorized lymph flow in a closed system. We describe our experiences with four dogs and we discuss proposed immunologic manipulation.", "contents": "Exteriorization of thoracic duct lymph. Theoretical considerations and an experimental model. Successful manipulation of the immune response will ultimately provide the answers for controlling homograft rejection, reversing autoimmune states, and quite possibly for curing carcinoma and other malignant neoplasms. During the past decade, many new drugs and techniques have been developed that alter the immune state. The theoretical basis for approaching the immune system via the thoracic duct is based on the fact that 90% to 95% of the cells in thoracic duct lymph are thymus-derived lymphocytes. Therefore, by tapping the thoracic duct, we could conceivably manipulate the T lymphocytes and control the cell-mediated arm of the immune system. This has in fact been done in renal transplantation and autoimmune diseases. An experimental canine model was developed that exteriorized lymph flow in a closed system. We describe our experiences with four dogs and we discuss proposed immunologic manipulation."}
{"id": "PMID:1081877", "title": "[A novel model of experimental toxic hepatitis/acute degenerative hepatitis induced by frog virus 3 (FV3) in the mouse (author's transl)].", "content": "The i.p. or i.v. injection of frog virus 3 (FV3) in mice produces a hepatitis which leads to the death of the animals within 24 h. This hepatitis is of a purely toxic nature since the virus does not develop at 37 degrees C. The toxic effect of the virus, which can be differentiated from the infectious effect, involves one or more structural proteins. The first pathological changes occur during the first few hours after the injection in the vicinity of the nuclei of the liver parenchyma cells in the form of changes in the chromatin and nucleoplasm. The inhibition of the synthesis of cellular macromolecules and of the function of nuclear enzymes points to the fact that it is the nucleus that is first and foremost attacked. Necrosis and biochemical disturbances in the vicinity of the cytoplasm appear later on. Premedication of the mice with a water-soluble silymarin salt leads to a distinct rise in the survival rate of the animals. The protective function of silymarin is dependent on the dose and on the duration of the premedication. The LD50 of FV3 in those mice which had previously been given silymarin, is approximately three times that of the animals which received no premedication.", "contents": "[A novel model of experimental toxic hepatitis/acute degenerative hepatitis induced by frog virus 3 (FV3) in the mouse (author's transl)]. The i.p. or i.v. injection of frog virus 3 (FV3) in mice produces a hepatitis which leads to the death of the animals within 24 h. This hepatitis is of a purely toxic nature since the virus does not develop at 37 degrees C. The toxic effect of the virus, which can be differentiated from the infectious effect, involves one or more structural proteins. The first pathological changes occur during the first few hours after the injection in the vicinity of the nuclei of the liver parenchyma cells in the form of changes in the chromatin and nucleoplasm. The inhibition of the synthesis of cellular macromolecules and of the function of nuclear enzymes points to the fact that it is the nucleus that is first and foremost attacked. Necrosis and biochemical disturbances in the vicinity of the cytoplasm appear later on. Premedication of the mice with a water-soluble silymarin salt leads to a distinct rise in the survival rate of the animals. The protective function of silymarin is dependent on the dose and on the duration of the premedication. The LD50 of FV3 in those mice which had previously been given silymarin, is approximately three times that of the animals which received no premedication."}
{"id": "PMID:1081878", "title": "Some biological properties of flurbiprofen, an anti-inflammatory, analgesic and antipyretic agent.", "content": "2-(2-Fluoro-4-biphenyl)propionic acid (flurbiprofen) possesses peripheral analgesic, anti-inflammatory and antipyretic properties. It does not possess glucocorticoid or adrenocortical-stimulating properties. It is a highly potent agent which in acute pharmacological test systems produced a significant pharmacological effect in single oral doses varying from 0.04 to 0.47 mg/kg. The peak plasma concentrations attained after these doses were generally of the order of 1 to 3 mug/ml. Doses of 0.33 mg/kg/day, which gave peak plasma concentrations of 0.6 mug/ml, produced a significant inhibition of rat adjuvant arthritis, both developing and established. The very shallow dose-response curves for flurbiprofen compared with acetylsalicylic acid, especially in the mouse and the rat test systems, are not due to an unreliable or abnormal absorption, which suggests that in these species the mode of action of flurbiprofen is not identical with that of acetylsalicylic acid.", "contents": "Some biological properties of flurbiprofen, an anti-inflammatory, analgesic and antipyretic agent. 2-(2-Fluoro-4-biphenyl)propionic acid (flurbiprofen) possesses peripheral analgesic, anti-inflammatory and antipyretic properties. It does not possess glucocorticoid or adrenocortical-stimulating properties. It is a highly potent agent which in acute pharmacological test systems produced a significant pharmacological effect in single oral doses varying from 0.04 to 0.47 mg/kg. The peak plasma concentrations attained after these doses were generally of the order of 1 to 3 mug/ml. Doses of 0.33 mg/kg/day, which gave peak plasma concentrations of 0.6 mug/ml, produced a significant inhibition of rat adjuvant arthritis, both developing and established. The very shallow dose-response curves for flurbiprofen compared with acetylsalicylic acid, especially in the mouse and the rat test systems, are not due to an unreliable or abnormal absorption, which suggests that in these species the mode of action of flurbiprofen is not identical with that of acetylsalicylic acid."}
{"id": "PMID:1081879", "title": "Two-dimensional immunoelectrophoresis: a new simplified method.", "content": "A new method of two-dimensional immunoelectrophoresis is described, in which fixed amounts of both antigen (total proteins) and antibody are employed. When applied to normal human sera, it results in the appearance of 15 peaks accounting for the commonest serum proteins with the only exception of alpha 1-acid glycoprotein. Transferrin, measured by Laurell's or Mancini's technique, is used as an internal standard. The height of the transferrin peak at 300 mg% is determined by a calibration curve. The heights of the various peaks are thus expressed as percentages of that of transferrin at 300 mg%. The method appears to be simple, rapid and antiserum-saving.", "contents": "Two-dimensional immunoelectrophoresis: a new simplified method. A new method of two-dimensional immunoelectrophoresis is described, in which fixed amounts of both antigen (total proteins) and antibody are employed. When applied to normal human sera, it results in the appearance of 15 peaks accounting for the commonest serum proteins with the only exception of alpha 1-acid glycoprotein. Transferrin, measured by Laurell's or Mancini's technique, is used as an internal standard. The height of the transferrin peak at 300 mg% is determined by a calibration curve. The heights of the various peaks are thus expressed as percentages of that of transferrin at 300 mg%. The method appears to be simple, rapid and antiserum-saving."}
{"id": "PMID:1081876", "title": "Association of cold-reactive antilymphocyte antibodies with lymphopenia in systemic lupus erythematosus.", "content": "In a prospective study 26 of 29 patients with systemic lupus erythematosus had cold-reactive antilymphocyte antibodies cytotoxic for autologous lymphocytes and lymphocytes from normal subjects. The level of antilymphocyte antibodies was highly correlated, by linear regression analysis, with lymphopenia in these patients. The data suggested that both the avidity and the concentration of these antibodies were important determinants in this relationship. A clear association between increased antilymphocyte antibody activity and exacerbation of SLE was demonstrated. Apart from lymphopenia, however, neither type of clinical manifestation nor any particular serologic abnormality appeared to be related to the presence of antilymphocyte antibodies.", "contents": "Association of cold-reactive antilymphocyte antibodies with lymphopenia in systemic lupus erythematosus. In a prospective study 26 of 29 patients with systemic lupus erythematosus had cold-reactive antilymphocyte antibodies cytotoxic for autologous lymphocytes and lymphocytes from normal subjects. The level of antilymphocyte antibodies was highly correlated, by linear regression analysis, with lymphopenia in these patients. The data suggested that both the avidity and the concentration of these antibodies were important determinants in this relationship. A clear association between increased antilymphocyte antibody activity and exacerbation of SLE was demonstrated. Apart from lymphopenia, however, neither type of clinical manifestation nor any particular serologic abnormality appeared to be related to the presence of antilymphocyte antibodies."}
{"id": "PMID:1081875", "title": "Hemorrhagic enteritis of turkeys. Antiserum -- efficacy, preparation, and use.", "content": "Intestinal and splenic lesions of experimentally produced hemorrhagic enteritis of turkeys were prevented by administering convalescent antiserum. Protection varied with antiserum quality and time of administration after infection. In general, 1.0 to 0.5 ml per poult prevented all lesions and as little as 0.25 to 0.1 ml prevented intestinal lesions when administered within 48 hours of injection of the virus inoculum. Adding phenol to antiserum did not reduce its effectiveness. Convalescent antiserum was useful in controlling field hemorrhagic enteritis.", "contents": "Hemorrhagic enteritis of turkeys. Antiserum -- efficacy, preparation, and use. Intestinal and splenic lesions of experimentally produced hemorrhagic enteritis of turkeys were prevented by administering convalescent antiserum. Protection varied with antiserum quality and time of administration after infection. In general, 1.0 to 0.5 ml per poult prevented all lesions and as little as 0.25 to 0.1 ml prevented intestinal lesions when administered within 48 hours of injection of the virus inoculum. Adding phenol to antiserum did not reduce its effectiveness. Convalescent antiserum was useful in controlling field hemorrhagic enteritis."}
{"id": "PMID:1081882", "title": "The nature of a pgiment-protein complex excreted from mutants of Rhodopseudomonas sphaeroides.", "content": "Pigment-protein complexes excreted from three bacteriochlorophyll-less mutants (mutants 8, 8-29, and 8-47) of Rhodopseudomonas sphaeroides have been isolated and purified. In the absence of detergents the complexes remained in an aggregated state, but were disaggregated by 0.2% Triron X-100. Sepharose 6B gel filtration indicated that the disaggregated complex from each of the mutants had a particle weight of about 165000, and contained 30 +/- 3% protein. This complex was further dissociated by 1% sodium dodecyl sulfate. Sephadex G-100 gel filtration now indicated that the majority of the protein was present as a small polypeptide with a molecular weight of about 9000. The pigment-protein complex from one of the mutants was treated with a bacteriochlorophyll extract. The bacteriochlorophyll was converted to bacteriopheophytin and became bound to the protein, replacing the endogenous tetrapyrrole (a bacteriocholorophyll precursor). The red absorption maximum of the bacteriopheophytin was shifted during this process to 840-865 nm. These properties are consistent with the possibility that the pigment-protein complexes contain a protein normally associated with light-harvesting bacteriochlorophyll in the wide-type strain.", "contents": "The nature of a pgiment-protein complex excreted from mutants of Rhodopseudomonas sphaeroides. Pigment-protein complexes excreted from three bacteriochlorophyll-less mutants (mutants 8, 8-29, and 8-47) of Rhodopseudomonas sphaeroides have been isolated and purified. In the absence of detergents the complexes remained in an aggregated state, but were disaggregated by 0.2% Triron X-100. Sepharose 6B gel filtration indicated that the disaggregated complex from each of the mutants had a particle weight of about 165000, and contained 30 +/- 3% protein. This complex was further dissociated by 1% sodium dodecyl sulfate. Sephadex G-100 gel filtration now indicated that the majority of the protein was present as a small polypeptide with a molecular weight of about 9000. The pigment-protein complex from one of the mutants was treated with a bacteriochlorophyll extract. The bacteriochlorophyll was converted to bacteriopheophytin and became bound to the protein, replacing the endogenous tetrapyrrole (a bacteriocholorophyll precursor). The red absorption maximum of the bacteriopheophytin was shifted during this process to 840-865 nm. These properties are consistent with the possibility that the pigment-protein complexes contain a protein normally associated with light-harvesting bacteriochlorophyll in the wide-type strain."}
{"id": "PMID:1081884", "title": "[Spontaneous and induced autoxidation of phospholipids during conformational rearrangements in outer segment membranes of frog retinal rods].", "content": "Kinetics of spontaneous and induced by the system Fe+++ascorbate peroxide oxidation of lipids in the membranes of outer segments of frog retina rods has been studied. Free radical oxidation of phospholipids passes the same stages as liquid phase oxidation of alkenes. Docosahexaenoi fatty acid residue of phosphatidyletanolamine is the main substrate of autooxidation. The structural organization of photoreceptor membranes is the factor which controls the reaction rate of peroxide oxidation; and reconstructions in membrane organization (at rhodopsin photolysis) result in the changes of autooxidation rate of phospholipids in the membrane.", "contents": "[Spontaneous and induced autoxidation of phospholipids during conformational rearrangements in outer segment membranes of frog retinal rods]. Kinetics of spontaneous and induced by the system Fe+++ascorbate peroxide oxidation of lipids in the membranes of outer segments of frog retina rods has been studied. Free radical oxidation of phospholipids passes the same stages as liquid phase oxidation of alkenes. Docosahexaenoi fatty acid residue of phosphatidyletanolamine is the main substrate of autooxidation. The structural organization of photoreceptor membranes is the factor which controls the reaction rate of peroxide oxidation; and reconstructions in membrane organization (at rhodopsin photolysis) result in the changes of autooxidation rate of phospholipids in the membrane."}
{"id": "PMID:1081883", "title": "[Protein-lipid interactions during lipid peroxidation in photoreceptor membranes].", "content": "It has been shown that chemical modification of hydrophobic lipid phase (lipoperoxidation) of outer segments membrane discs of frog retina rods brings about weakening protein-lipid interactions in the photoreceptor membrane. As a result of this extraction of rhodopsin with anion detergent (sodium cholate) in the concentrations not exceeding the critical concentration of micelloformation increases, and spontaneous release of rhodopsin into water phase is observed. At the same time the number of phospholipid molecules extracted in lipoprotein rhodopsin complex from the membranes of outer segments decreases 3-4-fold.", "contents": "[Protein-lipid interactions during lipid peroxidation in photoreceptor membranes]. It has been shown that chemical modification of hydrophobic lipid phase (lipoperoxidation) of outer segments membrane discs of frog retina rods brings about weakening protein-lipid interactions in the photoreceptor membrane. As a result of this extraction of rhodopsin with anion detergent (sodium cholate) in the concentrations not exceeding the critical concentration of micelloformation increases, and spontaneous release of rhodopsin into water phase is observed. At the same time the number of phospholipid molecules extracted in lipoprotein rhodopsin complex from the membranes of outer segments decreases 3-4-fold."}
{"id": "PMID:1081889", "title": "Electrical characteristics of frog atrial trabeculae in the double sucrose gap.", "content": "The electrical behavior of small single frog atrial trabeculae in the double sucrose gap has been investigated. The currents injected during voltage clamp experiments did not behave as predicted from the assumption of spatial uniformity of the voltage across a Hodgkin-Huxley membrane. Much of the difference is due to the geometrical complexities of this tissue. Nonetheless, two transient inward currents have been identified, the faster of which is blocked by tetrodotoxin (TTX). The magnitude of the slower transient varies markedly between preparations but always increases in a given preparation with increase of external calcium. The fast transient current traces, at small to intermediate depolarizations, are often marred by the presence of notches and secondary peaks due most probably to the loss of space clamp conditions. In many preparations these could be removed by reducing the current magnitude through application of a partially-blocking dose of TTX. Conversely, in the preparations whose fast transient was fully blocked by TTX, notches and secondary peaks in the slow transient could by induced through increasing calcium concentration and thereby the slow current magnitude. Previously used techniques for the measurement of the reversal potential of the fast inward transient have been shown to be invalid. In so far as they can be measured, the reversal potentials of the fast and slow inward transient are in the same neighborhood, i.e. around 120 mV from rest. The true values may be quite a bit apart. The total charge flow in the capacitive transient was measured for different sized nodes and preparations. From these data and estimates of plasma membrane area per unit trabecular volume, specific membrane capacitances of around 3 muF/cm2 were calculated for small bundles. The apparent ion current densities on this basis are approximately 1/10 of those measured in axons. The capacitive current occurring in small bundles decayed as the sum of at least three exponential functions of time. On the basis of these data and the anomalously large stable node widths, we suggest a coaxial core model of the preparation with the inner elements in series with an additional large extracellular resistance.", "contents": "Electrical characteristics of frog atrial trabeculae in the double sucrose gap. The electrical behavior of small single frog atrial trabeculae in the double sucrose gap has been investigated. The currents injected during voltage clamp experiments did not behave as predicted from the assumption of spatial uniformity of the voltage across a Hodgkin-Huxley membrane. Much of the difference is due to the geometrical complexities of this tissue. Nonetheless, two transient inward currents have been identified, the faster of which is blocked by tetrodotoxin (TTX). The magnitude of the slower transient varies markedly between preparations but always increases in a given preparation with increase of external calcium. The fast transient current traces, at small to intermediate depolarizations, are often marred by the presence of notches and secondary peaks due most probably to the loss of space clamp conditions. In many preparations these could be removed by reducing the current magnitude through application of a partially-blocking dose of TTX. Conversely, in the preparations whose fast transient was fully blocked by TTX, notches and secondary peaks in the slow transient could by induced through increasing calcium concentration and thereby the slow current magnitude. Previously used techniques for the measurement of the reversal potential of the fast inward transient have been shown to be invalid. In so far as they can be measured, the reversal potentials of the fast and slow inward transient are in the same neighborhood, i.e. around 120 mV from rest. The true values may be quite a bit apart. The total charge flow in the capacitive transient was measured for different sized nodes and preparations. From these data and estimates of plasma membrane area per unit trabecular volume, specific membrane capacitances of around 3 muF/cm2 were calculated for small bundles. The apparent ion current densities on this basis are approximately 1/10 of those measured in axons. The capacitive current occurring in small bundles decayed as the sum of at least three exponential functions of time. On the basis of these data and the anomalously large stable node widths, we suggest a coaxial core model of the preparation with the inner elements in series with an additional large extracellular resistance."}
{"id": "PMID:1081887", "title": "[Effect of trypsin inhibitor of a peptide-protein nature on kallikreins from human and rabbit blood stream].", "content": "The effect of three natural trypsin inhibitors--polyvalent Kunitz inhibitor (BPTI), the inhibitor from cow colostrum (CTI) and the inhibitor from soybean (SBTI)--on esterase and kininogenase action of partially purified kallikrein preparations from human and rabbit blood serum is studied. The effect of each inhibitor was estimated from Ki values. The latters show that BPTI, SBTI and CTI are strong inhibitors of both kallikreins. Ki values, as estimated from the hydrolysis rate of N-bensoyl-L-arginine ethyl ester, were found to be for human blood serum kallikrein and BPTI, SBTI and CTI 1,1-10(-9), 4,7-10(-9) and 3,6-10(-8) M respectively and for rabbit kallikrein--1,7-10(-9), 2,3-10(-8) and 2,3-10(-8) M. In the case of kallikrein catalysing more specific kininogenase reaction Ki value for complex of human serum kallikrein with BPTI was 4,8-10(-10) M, for SBTI--1,1-10(-10) M and for CTI--3,6-10(-8) M; for rabbit kallikrein--1,7-10(-9) M, 1,1-10(-9) and 2,3-10(-8) M respectively. The data obtained suggest the high sensitivity of human and rabbit serum kallikreins to the trypsin inhibitor of peptide-protein nature and a close similarity in composition of the active site for both serum kallikreins and trypsin, two spices different kininogenases--from human and rabbit serum had also similarity in molecule conformation and composition active site.", "contents": "[Effect of trypsin inhibitor of a peptide-protein nature on kallikreins from human and rabbit blood stream]. The effect of three natural trypsin inhibitors--polyvalent Kunitz inhibitor (BPTI), the inhibitor from cow colostrum (CTI) and the inhibitor from soybean (SBTI)--on esterase and kininogenase action of partially purified kallikrein preparations from human and rabbit blood serum is studied. The effect of each inhibitor was estimated from Ki values. The latters show that BPTI, SBTI and CTI are strong inhibitors of both kallikreins. Ki values, as estimated from the hydrolysis rate of N-bensoyl-L-arginine ethyl ester, were found to be for human blood serum kallikrein and BPTI, SBTI and CTI 1,1-10(-9), 4,7-10(-9) and 3,6-10(-8) M respectively and for rabbit kallikrein--1,7-10(-9), 2,3-10(-8) and 2,3-10(-8) M. In the case of kallikrein catalysing more specific kininogenase reaction Ki value for complex of human serum kallikrein with BPTI was 4,8-10(-10) M, for SBTI--1,1-10(-10) M and for CTI--3,6-10(-8) M; for rabbit kallikrein--1,7-10(-9) M, 1,1-10(-9) and 2,3-10(-8) M respectively. The data obtained suggest the high sensitivity of human and rabbit serum kallikreins to the trypsin inhibitor of peptide-protein nature and a close similarity in composition of the active site for both serum kallikreins and trypsin, two spices different kininogenases--from human and rabbit serum had also similarity in molecule conformation and composition active site."}
{"id": "PMID:1081891", "title": "Impaired T-cell transformation in chronic lymphocytic leukemia (CLL): Demonstration of a blastogenesis inhibitory factor.", "content": "A noncytotoxic serum factor was found in CLL serum which interfered with the response of normal lymphocytes to phytohemagglutinin (PHA) and pokeweed mitogen (PWM). This factor was not produced by B-lymphocytes during short-term culture. This factor may contribute to the impaired lymphocyte responsiveness in CLL. In five of six patients, T-lymphocytes which were purified by passage over nylon wool columns exhibited an impaired response to both PHA and PWM, suggesting that a dilutional effect alone does not fully explain the impaired lymphocyte transformation in CLL.", "contents": "Impaired T-cell transformation in chronic lymphocytic leukemia (CLL): Demonstration of a blastogenesis inhibitory factor. A noncytotoxic serum factor was found in CLL serum which interfered with the response of normal lymphocytes to phytohemagglutinin (PHA) and pokeweed mitogen (PWM). This factor was not produced by B-lymphocytes during short-term culture. This factor may contribute to the impaired lymphocyte responsiveness in CLL. In five of six patients, T-lymphocytes which were purified by passage over nylon wool columns exhibited an impaired response to both PHA and PWM, suggesting that a dilutional effect alone does not fully explain the impaired lymphocyte transformation in CLL."}
{"id": "PMID:1081892", "title": "Growth stimulation of human bone marrow cells in agar culture by vascular cells.", "content": "Human vascular cells are capable of stimulating granulopoiesis in agar culture of human bone marrow cells. This effect was obtained by including vein fragments in the culture or by using endothelial cells separated from the vein of human umbilical cords as feeder cells. Furthermore, the stimulatory capacity of conditioned medium obtained from cord veins was found to be highly active in comparison to that obtained from peripheral leukocytes. Endothelial cells within the bond marrow cavity are suggested as a local source of factors regulating granulopoiesis in humans in addition to the monocyte.", "contents": "Growth stimulation of human bone marrow cells in agar culture by vascular cells. Human vascular cells are capable of stimulating granulopoiesis in agar culture of human bone marrow cells. This effect was obtained by including vein fragments in the culture or by using endothelial cells separated from the vein of human umbilical cords as feeder cells. Furthermore, the stimulatory capacity of conditioned medium obtained from cord veins was found to be highly active in comparison to that obtained from peripheral leukocytes. Endothelial cells within the bond marrow cavity are suggested as a local source of factors regulating granulopoiesis in humans in addition to the monocyte."}
{"id": "PMID:1081893", "title": "Terminal deoxynucleotidyl transferase activities in human blood leukocytes and lymphoblast cell lines: high levels in lymphoblast cell lines and in blast cells of some patients with chronic myelogenous leukemia in acute phase.", "content": "Terminal deoxynucleotidyl transferase, an enzyme which catalyzes the polymerization of deoxyribonucleoside triphosphates, elongating oligo- or polydeoxynucleotide chains, but without direction from a nucleic acid template, is thought to be specific for thymus gland and thymus-derived cells. We have confirmed the observations that high levels are characteristic of thymus gland with both human and calf tissue and that elevated levels may be found in some cases of acute lymphocytic leukemia. High levels were also found in human lymphoblast cell lines with T-cell characteristics, and insignificant activity was observed in leukocytes of patients with chronic myelogenous leukemia not in acute blast phase of the disease, chronic lymphocytic leukemia, human B-cells, and normal human blood lymphocytes even after stimulation with phytohemagglutinin. However, high levels (approximately 200 nmoles/hr/10(9) cells) equivalent to those in thymus tissue and lymphoblast cell lines with T-cell characteristics were found in the peripheral blood blast cells of four patients with chronic myelogenous leukemia in an acute blast phase of their disease. One hypothesis that may explain the present results is that in chronic myelogenous leukemia in acute blast phase of the disease the proliferative blast response may not always be myeloblasts but in some cases it may be lymphoblasts.", "contents": "Terminal deoxynucleotidyl transferase activities in human blood leukocytes and lymphoblast cell lines: high levels in lymphoblast cell lines and in blast cells of some patients with chronic myelogenous leukemia in acute phase. Terminal deoxynucleotidyl transferase, an enzyme which catalyzes the polymerization of deoxyribonucleoside triphosphates, elongating oligo- or polydeoxynucleotide chains, but without direction from a nucleic acid template, is thought to be specific for thymus gland and thymus-derived cells. We have confirmed the observations that high levels are characteristic of thymus gland with both human and calf tissue and that elevated levels may be found in some cases of acute lymphocytic leukemia. High levels were also found in human lymphoblast cell lines with T-cell characteristics, and insignificant activity was observed in leukocytes of patients with chronic myelogenous leukemia not in acute blast phase of the disease, chronic lymphocytic leukemia, human B-cells, and normal human blood lymphocytes even after stimulation with phytohemagglutinin. However, high levels (approximately 200 nmoles/hr/10(9) cells) equivalent to those in thymus tissue and lymphoblast cell lines with T-cell characteristics were found in the peripheral blood blast cells of four patients with chronic myelogenous leukemia in an acute blast phase of their disease. One hypothesis that may explain the present results is that in chronic myelogenous leukemia in acute blast phase of the disease the proliferative blast response may not always be myeloblasts but in some cases it may be lymphoblasts."}
{"id": "PMID:1081895", "title": "B lymphocyte subpopulations in chronic lymphocytic leukemia.", "content": "We have defined two subpopulations of B lymphocytes in chronic lymphocytic leukemia (CLL). The major variant (termed typical) was characterized by the presence of a relatively homogeneous population of small-to-medium-sized lymphocytes with low-density SmIgM and no evidence of intracellular Ig synthesis. A minor group (termed atypical) was identified by the presence of a pleomorphic cell population with few small lymphocytes. The predominant SmIg was IgG, which was detected intracellularly as well as in the serum. The atypical group appeared to be an arrest at a later stage of differentiation where a switch from IgM to IgG synthesis and secretion had occurred. Clinical correlation suggested several clear-cut differences in clinicopathologic characteristics, but the median survivals for both groups at 2 yr was nearly identical.", "contents": "B lymphocyte subpopulations in chronic lymphocytic leukemia. We have defined two subpopulations of B lymphocytes in chronic lymphocytic leukemia (CLL). The major variant (termed typical) was characterized by the presence of a relatively homogeneous population of small-to-medium-sized lymphocytes with low-density SmIgM and no evidence of intracellular Ig synthesis. A minor group (termed atypical) was identified by the presence of a pleomorphic cell population with few small lymphocytes. The predominant SmIg was IgG, which was detected intracellularly as well as in the serum. The atypical group appeared to be an arrest at a later stage of differentiation where a switch from IgM to IgG synthesis and secretion had occurred. Clinical correlation suggested several clear-cut differences in clinicopathologic characteristics, but the median survivals for both groups at 2 yr was nearly identical."}
{"id": "PMID:1081896", "title": "Immunologic studies on human factor VIII (anti-hemophilic factor A, AHF) components produced by low-ionic-strength dialysis.", "content": "Since dialysis of human factor VIII against buffers of low ionic strength yielded two distinct components, and since the factor VIII fraction isolated from normal plasma showed von Willebrand factor activity as defined by the corrective effect on abnormal platelet retention and ristocetin aggregation in von Willebrand's disease, the present studies were performed to determine if the correcting activities could be attributed to one or both of the components. Dialysis of factor VIII against buffers of low ionic strength led, however, to a decrease in factor VIII procoagulant activity and the reduction of the correcting activities, which suggested that the intact aggregate was required for procoagulant activity and for von Willebrand factor activity. In this respect dialysis of factor VIII at low ionic strength differed from dissociation at high salt concentrations. The two low ionic strength components were identified by the use of a rabbit antiserum against factor VIII, and could be distinguished on the basis of specific antigenic structures. Dialysis of factor VIII at low ionic strength led to a decrease in antigenic determinants closely related to factor VIII function. Specific antibodies to the low ionic strength components inhibited factor VIII activity in normal plasma, but the residual factor VIII was higher than that after inhibition with antibodies against intact factor VIII. Both antibodies interfered with von Willebrand factor activity.", "contents": "Immunologic studies on human factor VIII (anti-hemophilic factor A, AHF) components produced by low-ionic-strength dialysis. Since dialysis of human factor VIII against buffers of low ionic strength yielded two distinct components, and since the factor VIII fraction isolated from normal plasma showed von Willebrand factor activity as defined by the corrective effect on abnormal platelet retention and ristocetin aggregation in von Willebrand's disease, the present studies were performed to determine if the correcting activities could be attributed to one or both of the components. Dialysis of factor VIII against buffers of low ionic strength led, however, to a decrease in factor VIII procoagulant activity and the reduction of the correcting activities, which suggested that the intact aggregate was required for procoagulant activity and for von Willebrand factor activity. In this respect dialysis of factor VIII at low ionic strength differed from dissociation at high salt concentrations. The two low ionic strength components were identified by the use of a rabbit antiserum against factor VIII, and could be distinguished on the basis of specific antigenic structures. Dialysis of factor VIII at low ionic strength led to a decrease in antigenic determinants closely related to factor VIII function. Specific antibodies to the low ionic strength components inhibited factor VIII activity in normal plasma, but the residual factor VIII was higher than that after inhibition with antibodies against intact factor VIII. Both antibodies interfered with von Willebrand factor activity."}
{"id": "PMID:1081897", "title": "Platelet release abnormality associated with a variant of von Willebrand's disease.", "content": "A family with a platelet release abnormality (PRA) is described. The only son also showed a reduced rate of platelet aggregation in response to ristocetin, markedly reduced levels of von Willebrand's factor (vWf, ristocetin cofactor), and increased mobility of factor VIII-like antigen, features which were suggestive of von Willebrand's disease (vWd). No inhibition of vWf was found in his plasma. Family studies showed no evidence of vWd in the mother. The father's investigations showed a low rate of ristocetin aggregation on one of the two occasions when it was tested and low vWf on two of four occasions. Despite repeated testing, the findings in the father did not conclusively rule out the possibility of mild vWd, and it was impossible to determine whether the vWd in the son was inherited or arose as a mutation. The findings in this family suggest a possible relationship between abnormalities of the factor VIII complex and defective platelet function.", "contents": "Platelet release abnormality associated with a variant of von Willebrand's disease. A family with a platelet release abnormality (PRA) is described. The only son also showed a reduced rate of platelet aggregation in response to ristocetin, markedly reduced levels of von Willebrand's factor (vWf, ristocetin cofactor), and increased mobility of factor VIII-like antigen, features which were suggestive of von Willebrand's disease (vWd). No inhibition of vWf was found in his plasma. Family studies showed no evidence of vWd in the mother. The father's investigations showed a low rate of ristocetin aggregation on one of the two occasions when it was tested and low vWf on two of four occasions. Despite repeated testing, the findings in the father did not conclusively rule out the possibility of mild vWd, and it was impossible to determine whether the vWd in the son was inherited or arose as a mutation. The findings in this family suggest a possible relationship between abnormalities of the factor VIII complex and defective platelet function."}
{"id": "PMID:1081900", "title": "The immunological hazard of Cushing's syndrome.", "content": "A 24-year-old woman was found to have cryptococcal meningitis and Cushing's syndrome due to an adrenal adenoma. Her meningitis was successfully arrested with fluorouracil. Treatment with metyrapone decreased her cortisol production and produced clinical remission of Cushing's syndrome. On admission her peripheral T lymphocytes were few and hyporeactive. When the overproduction of cortisol ceased the numbers of T lymphocytes and their reactivity returned to normal and she developed in-vitro lymphocyte responsiveness to the cryptococci.", "contents": "The immunological hazard of Cushing's syndrome. A 24-year-old woman was found to have cryptococcal meningitis and Cushing's syndrome due to an adrenal adenoma. Her meningitis was successfully arrested with fluorouracil. Treatment with metyrapone decreased her cortisol production and produced clinical remission of Cushing's syndrome. On admission her peripheral T lymphocytes were few and hyporeactive. When the overproduction of cortisol ceased the numbers of T lymphocytes and their reactivity returned to normal and she developed in-vitro lymphocyte responsiveness to the cryptococci."}
{"id": "PMID:1081901", "title": "In vivo release of 5-HT in the lateral ventricle of the rat: effects of 5-hydroxytryptophan and tryptophan.", "content": "The in vivo release of 5-HT was examined in the rat brain. For this purpose, the left lateral ventricle was perfused at a constant rate with an artificial CSF for several hours in animals anaesthetized with halothane. 5-HT was estimated in serial 1-h collected fractions. The amine was first isolated by adsorption on a Sephadex G-10 column and then assayed using the radioenzymatic method of Saavedra et al.37, slightly modified to improve its sensitivity. The quantity of 5-HT released spontaneously during the first hour fraction was 296 pg, it was lower (99 pg/h) in the following fractions. 5-HT released into the CSF may in great part originate from serotoninergic terminals localized in structures surrounding the ventricle. This was suggested by experiments in which exogenous [3H]5-HT or [3H]tryptophan were perfused through the lateral ventricle during a few hours. [3H]5-HT taken up or synthetized was mainly localized in structures surrounding the ventricular space. The acute injection of 5-hydroxytryptophan (100 mg/kg) induced an immediate important and long lasting increase of 5-HT release. In contrast the acute injection of tryptophan (100 mg/kg) led to a transient and moderate elevation of 5-HT release which was only detected during the second hour of perfusion. Curiously a similar pattern of transmitter release was observed following the constant intravenous infusion of the amino acid (70 mg/kg/h) except that the increase in 5-HT release was much more pronounced during the second hour than after the acute injection. Parallel experiments were made to determine the time course of the changes of free and total tryptophan levels in plasma and of those of tryptophan, 5-HT, and 5-hydroxyindoleacetic-acid (5-HIAA) in brain tissues, induced by the acute and long term administrations of tryptophan. Moreover the rate of 5-HT synthesis was estimated using the monoamine oxidase inhibition method 2 and 5 h after both tryptophan treatments in halothane anaesthetized rats. 5-HT levels and the synthesis rate of the transmitter were increased at 2 h (when both tryptophan treatments stimulated 5-HT release). Despite the presence of high tryptophan levels in plasma and tissues and of high 5-HT and 5-HIAA levels in tissues, the synthesis rate of 5-HT (as the 5-HT release) was similar to that of controls 5 h after the onset of tryptophan infusion. These results suggest that some relationships occurred between the changes in 5-HT SYNTHESIs and release after the first hour of perfusion. The absence of effects of tryptophan treatments on 5-HT release during the first hour of perfusion are also discussed.", "contents": "In vivo release of 5-HT in the lateral ventricle of the rat: effects of 5-hydroxytryptophan and tryptophan. The in vivo release of 5-HT was examined in the rat brain. For this purpose, the left lateral ventricle was perfused at a constant rate with an artificial CSF for several hours in animals anaesthetized with halothane. 5-HT was estimated in serial 1-h collected fractions. The amine was first isolated by adsorption on a Sephadex G-10 column and then assayed using the radioenzymatic method of Saavedra et al.37, slightly modified to improve its sensitivity. The quantity of 5-HT released spontaneously during the first hour fraction was 296 pg, it was lower (99 pg/h) in the following fractions. 5-HT released into the CSF may in great part originate from serotoninergic terminals localized in structures surrounding the ventricle. This was suggested by experiments in which exogenous [3H]5-HT or [3H]tryptophan were perfused through the lateral ventricle during a few hours. [3H]5-HT taken up or synthetized was mainly localized in structures surrounding the ventricular space. The acute injection of 5-hydroxytryptophan (100 mg/kg) induced an immediate important and long lasting increase of 5-HT release. In contrast the acute injection of tryptophan (100 mg/kg) led to a transient and moderate elevation of 5-HT release which was only detected during the second hour of perfusion. Curiously a similar pattern of transmitter release was observed following the constant intravenous infusion of the amino acid (70 mg/kg/h) except that the increase in 5-HT release was much more pronounced during the second hour than after the acute injection. Parallel experiments were made to determine the time course of the changes of free and total tryptophan levels in plasma and of those of tryptophan, 5-HT, and 5-hydroxyindoleacetic-acid (5-HIAA) in brain tissues, induced by the acute and long term administrations of tryptophan. Moreover the rate of 5-HT synthesis was estimated using the monoamine oxidase inhibition method 2 and 5 h after both tryptophan treatments in halothane anaesthetized rats. 5-HT levels and the synthesis rate of the transmitter were increased at 2 h (when both tryptophan treatments stimulated 5-HT release). Despite the presence of high tryptophan levels in plasma and tissues and of high 5-HT and 5-HIAA levels in tissues, the synthesis rate of 5-HT (as the 5-HT release) was similar to that of controls 5 h after the onset of tryptophan infusion. These results suggest that some relationships occurred between the changes in 5-HT SYNTHESIs and release after the first hour of perfusion. The absence of effects of tryptophan treatments on 5-HT release during the first hour of perfusion are also discussed."}
{"id": "PMID:1081903", "title": "Improvement in delayed hypersensitivity in Hodgkin's disease with transfer factor: lymphapheresis and cellular immune reactions or normal donors.", "content": "Passive transfer of delayed hypersensitivity was achieved, with normal transfer factor, in patients with Hodgkin's disease in remission. The cellular immune responses of the recipients improved. It is suggested that, in addition to specific effect the transfer factor (or factors) has a nonspecific effect causing improvement in the state of delayed hypersensitivity of the recipient in general. The average number of E-rosette T lymphocytes was 46.3% after the transfer factor treatment in Hodgkin's disease. The control patients with Hodgkin's disease, not receiving transfer factor, had a value of 37.8%. Removal of 4.9 X 10(9) to 1.08 X 10(10) lymphocytes did not diminish the delayed hypersensitivity of the donor. Side effects attributable to transfer factor were not seen.", "contents": "Improvement in delayed hypersensitivity in Hodgkin's disease with transfer factor: lymphapheresis and cellular immune reactions or normal donors. Passive transfer of delayed hypersensitivity was achieved, with normal transfer factor, in patients with Hodgkin's disease in remission. The cellular immune responses of the recipients improved. It is suggested that, in addition to specific effect the transfer factor (or factors) has a nonspecific effect causing improvement in the state of delayed hypersensitivity of the recipient in general. The average number of E-rosette T lymphocytes was 46.3% after the transfer factor treatment in Hodgkin's disease. The control patients with Hodgkin's disease, not receiving transfer factor, had a value of 37.8%. Removal of 4.9 X 10(9) to 1.08 X 10(10) lymphocytes did not diminish the delayed hypersensitivity of the donor. Side effects attributable to transfer factor were not seen."}
{"id": "PMID:1081904", "title": "Complications of indwelling chemotherapy catheters.", "content": "Fifty consecutive chemotherapeutic infusions for cancer via precutaneously introduced arterial catheters were reviewed to determine the frequency of angiographic and clinical complications related to the indwelling catheter. Fibrin cloaking along the catheter was found in 20 patients studied by pull-out arteriography and was unassociated with clinical symptoms. Major thrombus formation occurred around the catheter tip in 28% of the infused vessels, all within the hepatic artery. Clinical symptoms developed in only three patients where thrombosis of the hepatic artery had propagated into the celiac axis. Systemic heparinization did not reduce the incidence of thrombus formation but did increase the incidence of mild gastrointestinal bleeding. Several factors may reduce the incidence of complications, such as puncture of a large artery, use of soft, pliable, and small diameter catheters, proper free position of the catheter in the infused vessel, regular angiographic checkups for catheters position, and early evidence of thrombus formation.", "contents": "Complications of indwelling chemotherapy catheters. Fifty consecutive chemotherapeutic infusions for cancer via precutaneously introduced arterial catheters were reviewed to determine the frequency of angiographic and clinical complications related to the indwelling catheter. Fibrin cloaking along the catheter was found in 20 patients studied by pull-out arteriography and was unassociated with clinical symptoms. Major thrombus formation occurred around the catheter tip in 28% of the infused vessels, all within the hepatic artery. Clinical symptoms developed in only three patients where thrombosis of the hepatic artery had propagated into the celiac axis. Systemic heparinization did not reduce the incidence of thrombus formation but did increase the incidence of mild gastrointestinal bleeding. Several factors may reduce the incidence of complications, such as puncture of a large artery, use of soft, pliable, and small diameter catheters, proper free position of the catheter in the infused vessel, regular angiographic checkups for catheters position, and early evidence of thrombus formation."}
{"id": "PMID:1081905", "title": "Intensity of immunosuppressive therapy and the incidence of Pneumocystis carinii pneumonitis.", "content": "One hundred and forty-nine children with acute lymphocytic leukemia treated according to a prospective protocol were randomized after induction of remission and central nervous system (CNS) irradiation to receive maintenance chemotherapy with 1, 2, 3, or 4 chemotherapy agents. The incidence of P. carinii pneumonitis (PCP) was 5.0, 2.3, 2.2, and 22.4%, respectively, during the period of maintenance therapy. An additional 31 patients enrolled in the same study were placed in special categories to receive three drugs for maintenance plus supplemental chemotherapy or irradiation because of CNS leukemia on admission, remission failure, ediastinal mass, or generalized lymphosarcoma without bone marrow involvement. The incidences of PCP in these groups were 16.7, 30.0, 35.7, and 0%, respectively, during the period of maintenance therapy.", "contents": "Intensity of immunosuppressive therapy and the incidence of Pneumocystis carinii pneumonitis. One hundred and forty-nine children with acute lymphocytic leukemia treated according to a prospective protocol were randomized after induction of remission and central nervous system (CNS) irradiation to receive maintenance chemotherapy with 1, 2, 3, or 4 chemotherapy agents. The incidence of P. carinii pneumonitis (PCP) was 5.0, 2.3, 2.2, and 22.4%, respectively, during the period of maintenance therapy. An additional 31 patients enrolled in the same study were placed in special categories to receive three drugs for maintenance plus supplemental chemotherapy or irradiation because of CNS leukemia on admission, remission failure, ediastinal mass, or generalized lymphosarcoma without bone marrow involvement. The incidences of PCP in these groups were 16.7, 30.0, 35.7, and 0%, respectively, during the period of maintenance therapy."}
{"id": "PMID:1081906", "title": "A study of childhood non-Hodgkin's lymphoma.", "content": "The pathologic and clinical features of 31 cases of childhood non-Hodgkin's lymphoma (NHL) were reviewed retrospectively using Rappaport's classification and a modification of the Ann Arbor staging system. Twenty-nine (93.5%) of the patients had diffuse and 2 (6.5%) had nodular lymphoma. Diffuse histiocytic lymphoma accounted for 10 cases (32.3%), diffuse undifferentiated for 9 (29%), and diffuse lymphocytic, poorly differentiated for 5 (16.1%). Five cases (16.1%) were unclassifiable. No cases of well-differenitated lymphocytic or mixed cell lymphoma were found. A modified classification was attempted, which included also large basophilic cell (LBC), convoluted T-lymphocytic (CTL), and Burkitt's lymphomas. These pathologic subgroups accounted for 35.4%, 16.1%, and 6.5% of the cases, respectively. The patients were almost equally divided between clinically localized and generalized stages, and their survival was stage-dependent. The overall survival was 32.3%; the 3-year survival was 50% for Stages I and II, compared to 7.7% for Stages III and IV. The gastrointestinal tract was the most common site of origin. In 22% of the cases, the disease originated in extra-lymphatic tissues. Central nervous syste, involvement occurred in 10 of 31 children (32%), and a leukemic picture developed in 6 of 31 (19%). The Ctl lymphomas were confined to the mediastinum, whereas the LBC lymphomas arose mostly in Waldeyer's ring and Peyer's patches. We conclude that the extent of the disease as determined by clinical staging had prognostic significance in childhood NHL. The prognostic value of the histological classification could not be clearly established from our data.", "contents": "A study of childhood non-Hodgkin's lymphoma. The pathologic and clinical features of 31 cases of childhood non-Hodgkin's lymphoma (NHL) were reviewed retrospectively using Rappaport's classification and a modification of the Ann Arbor staging system. Twenty-nine (93.5%) of the patients had diffuse and 2 (6.5%) had nodular lymphoma. Diffuse histiocytic lymphoma accounted for 10 cases (32.3%), diffuse undifferentiated for 9 (29%), and diffuse lymphocytic, poorly differentiated for 5 (16.1%). Five cases (16.1%) were unclassifiable. No cases of well-differenitated lymphocytic or mixed cell lymphoma were found. A modified classification was attempted, which included also large basophilic cell (LBC), convoluted T-lymphocytic (CTL), and Burkitt's lymphomas. These pathologic subgroups accounted for 35.4%, 16.1%, and 6.5% of the cases, respectively. The patients were almost equally divided between clinically localized and generalized stages, and their survival was stage-dependent. The overall survival was 32.3%; the 3-year survival was 50% for Stages I and II, compared to 7.7% for Stages III and IV. The gastrointestinal tract was the most common site of origin. In 22% of the cases, the disease originated in extra-lymphatic tissues. Central nervous syste, involvement occurred in 10 of 31 children (32%), and a leukemic picture developed in 6 of 31 (19%). The Ctl lymphomas were confined to the mediastinum, whereas the LBC lymphomas arose mostly in Waldeyer's ring and Peyer's patches. We conclude that the extent of the disease as determined by clinical staging had prognostic significance in childhood NHL. The prognostic value of the histological classification could not be clearly established from our data."}
{"id": "PMID:1081908", "title": "Assay for citrovorum factor (NSC-3590) in the presence of methotrexate (NSC-740).", "content": "Treatment of certain malignancies with high-dose methotrexate/citrovorum factor rescue has recently been adopted as an effective regimen. A microbiologic assay capable of detecting citrovorum factor in the presence of massive amounts of methotrexate has been developed using a strain of Pediococcus cerevisiae resistant to methotrexate. The assay described in this paper is an inexpensive and rapid method of studying the distribution kinetics of citrovorum factor.", "contents": "Assay for citrovorum factor (NSC-3590) in the presence of methotrexate (NSC-740). Treatment of certain malignancies with high-dose methotrexate/citrovorum factor rescue has recently been adopted as an effective regimen. A microbiologic assay capable of detecting citrovorum factor in the presence of massive amounts of methotrexate has been developed using a strain of Pediococcus cerevisiae resistant to methotrexate. The assay described in this paper is an inexpensive and rapid method of studying the distribution kinetics of citrovorum factor."}
{"id": "PMID:1081921", "title": "Antibody-dependent lymphocyte killer function in human immunodeficiency diseases.", "content": "Antibody-dependent cell immunity to the lymphocyte system (ABCIL) has been shown to be a function of a non-thymus-processed cell in the experimental animal. To evaluate its role in the human and to assess its clinical usefulness, we assessed ABCIL in twenty-five patients with various immunodeficiency (ID) syndromes. Our technique measures the lysis of 51Cr-labelled normal human lymphocytes coated with HL-A-specific antibody. Cytotoxicity is expressed as a percentage of 51Cr released after subtracting the spontaneous target cell release. Mean values in normals are 20+/-2 (s.e.). The ten patients with AB deficiency had a mean ABCIL of 7-9+/-2 (Pless than0-01). All eight patients with cellular ID had normal ABCIL (18+/-2), while the ten patients with combined ID had variable results. Effector cell function in the ABCIL test correlated (r=0-74; Pless than0-05) with the percentage of B cells in the peripheral blood. No correlation was found between ABCIL function and serum immunoglobulin levels or rosette-forming cells in the peripheral blood. There is a function for B lymphocytes other than as a precursor of antibody-synthesizing cells.", "contents": "Antibody-dependent lymphocyte killer function in human immunodeficiency diseases. Antibody-dependent cell immunity to the lymphocyte system (ABCIL) has been shown to be a function of a non-thymus-processed cell in the experimental animal. To evaluate its role in the human and to assess its clinical usefulness, we assessed ABCIL in twenty-five patients with various immunodeficiency (ID) syndromes. Our technique measures the lysis of 51Cr-labelled normal human lymphocytes coated with HL-A-specific antibody. Cytotoxicity is expressed as a percentage of 51Cr released after subtracting the spontaneous target cell release. Mean values in normals are 20+/-2 (s.e.). The ten patients with AB deficiency had a mean ABCIL of 7-9+/-2 (Pless than0-01). All eight patients with cellular ID had normal ABCIL (18+/-2), while the ten patients with combined ID had variable results. Effector cell function in the ABCIL test correlated (r=0-74; Pless than0-05) with the percentage of B cells in the peripheral blood. No correlation was found between ABCIL function and serum immunoglobulin levels or rosette-forming cells in the peripheral blood. There is a function for B lymphocytes other than as a precursor of antibody-synthesizing cells."}
{"id": "PMID:1081922", "title": "The effect of Freund's complete adjuvant on the cellular response in mice to sheep erythrocytes.", "content": "The effect of Freund's complete adjuvant on the cellular response in BALB/c mice to SRBC was studied using techniques based on immunocytoadherence (ICA), inhibition of ICA using an antiserum to the theta alloantigen, and immune adherence (IA). Particular attention was paid to the cellular morphology of the responding lymph nodes, details of which are described.", "contents": "The effect of Freund's complete adjuvant on the cellular response in mice to sheep erythrocytes. The effect of Freund's complete adjuvant on the cellular response in BALB/c mice to SRBC was studied using techniques based on immunocytoadherence (ICA), inhibition of ICA using an antiserum to the theta alloantigen, and immune adherence (IA). Particular attention was paid to the cellular morphology of the responding lymph nodes, details of which are described."}
{"id": "PMID:1081923", "title": "Electrophoresis of lymphoid cells. A study of Bruton type of agammaglobulinaemia, thymic dysplasia, chronic lymphatic leukaemia and of normal human thymocytes.", "content": "The mean electrophoretic mobility (EPM) of blood lymphocytes from a patient with Bruton type of agammaglobulinaemia was high with a unimodal distribution pattern. That of blood lymphocytes from a patient with severe thymic dysplasia was low, with a small faster group. The difference between the major groups was about 30%. Thymocytes had a mean EPM 25% lower than that of normal T cells. Blood and spleen cells in a chronic lymphatic leukaemic of B-cell origin had a mean EPM 35% higher than that of the majority of normal B cells.", "contents": "Electrophoresis of lymphoid cells. A study of Bruton type of agammaglobulinaemia, thymic dysplasia, chronic lymphatic leukaemia and of normal human thymocytes. The mean electrophoretic mobility (EPM) of blood lymphocytes from a patient with Bruton type of agammaglobulinaemia was high with a unimodal distribution pattern. That of blood lymphocytes from a patient with severe thymic dysplasia was low, with a small faster group. The difference between the major groups was about 30%. Thymocytes had a mean EPM 25% lower than that of normal T cells. Blood and spleen cells in a chronic lymphatic leukaemic of B-cell origin had a mean EPM 35% higher than that of the majority of normal B cells."}
{"id": "PMID:1081924", "title": "Susceptibility of inbred rat strains to experimental thyroiditis: quantitation of thyroglobulin-binding cells and assessment of T-cell function in susceptible and non-susceptible strains.", "content": "Ten inbred strains of rats were immunized with crude homologous thyroglobulin emulsified in Freund's complete adjuvant in order to investigate strain susceptibility to the induction of both thyroiditis and antibody to thyroglobulin. Two strains (LH and AUG) were found to be extremely susceptible and had 100% incidence of thyroid lesions which in general varied from moderate to very severe (mean index of pathology+/-SE, 2-5+/-0-2 and 2-1+/-0-4 respectively). One other strain (HL) also had 100% incidence of lesions but there were consistently mild in character (1-1+/-0-1). Two strains (DA and SD) were variable, with thyroid change varying from negative to severe. Three strains (LEW, WAG and PVG/c) had occasional lesions and the remaining two strains (AS and CAM) showed no thyroid change. Four strains (LH, AUG, HL and DA) consistently produced good antibody responses to thyroglobulin (mean titres+/-SE 7-3+/-0-3, 9-5+/-0-4, 6-9+/-0-3 and 6-6+/-0-5 respectively). In contrast WAG and CAM rats failed to develop autoantibody and the responses of AS, PVG/c and SD strain rats were quite variable. Although the autoantibody response generally correlated well with the presence of thyroiditis in a particular strain, LEW, AS and PVG/c rats often had good antibody levels with minimal thyroid lesions. Females of the most susceptible strains (LH and AUG) were found to have significantly more severe thyroid lesions and higher antibody titres to thyroglobulin than males. The most susceptible strains were all found to be of the Ag-B5 major histocompatibility genotype whilst the least susceptible were of the Ag-B2 genotype. However, wide interstrain variability was noted within the Ag-B5 genotype particularly with respect to the induction and extent of thyroid lesions. It was not found possible to relate the divergence in susceptibility between rat strains of Ag-B5 and Ag-B2 genotypes to differences in respective numbers of thyroglobulin-binding cells within the circulation of the non-immunized animal. Similarly, there were no differences in response between a susceptible (LH) and non-susceptible (CAM) strain to the phytomitogens PHA and Con A.", "contents": "Susceptibility of inbred rat strains to experimental thyroiditis: quantitation of thyroglobulin-binding cells and assessment of T-cell function in susceptible and non-susceptible strains. Ten inbred strains of rats were immunized with crude homologous thyroglobulin emulsified in Freund's complete adjuvant in order to investigate strain susceptibility to the induction of both thyroiditis and antibody to thyroglobulin. Two strains (LH and AUG) were found to be extremely susceptible and had 100% incidence of thyroid lesions which in general varied from moderate to very severe (mean index of pathology+/-SE, 2-5+/-0-2 and 2-1+/-0-4 respectively). One other strain (HL) also had 100% incidence of lesions but there were consistently mild in character (1-1+/-0-1). Two strains (DA and SD) were variable, with thyroid change varying from negative to severe. Three strains (LEW, WAG and PVG/c) had occasional lesions and the remaining two strains (AS and CAM) showed no thyroid change. Four strains (LH, AUG, HL and DA) consistently produced good antibody responses to thyroglobulin (mean titres+/-SE 7-3+/-0-3, 9-5+/-0-4, 6-9+/-0-3 and 6-6+/-0-5 respectively). In contrast WAG and CAM rats failed to develop autoantibody and the responses of AS, PVG/c and SD strain rats were quite variable. Although the autoantibody response generally correlated well with the presence of thyroiditis in a particular strain, LEW, AS and PVG/c rats often had good antibody levels with minimal thyroid lesions. Females of the most susceptible strains (LH and AUG) were found to have significantly more severe thyroid lesions and higher antibody titres to thyroglobulin than males. The most susceptible strains were all found to be of the Ag-B5 major histocompatibility genotype whilst the least susceptible were of the Ag-B2 genotype. However, wide interstrain variability was noted within the Ag-B5 genotype particularly with respect to the induction and extent of thyroid lesions. It was not found possible to relate the divergence in susceptibility between rat strains of Ag-B5 and Ag-B2 genotypes to differences in respective numbers of thyroglobulin-binding cells within the circulation of the non-immunized animal. Similarly, there were no differences in response between a susceptible (LH) and non-susceptible (CAM) strain to the phytomitogens PHA and Con A."}
{"id": "PMID:1081925", "title": "Cell-mediated immunity in rubella assayed by cytotoxicity of supernatants from rubella virus-stimulated human lymphocyte cultures.", "content": "Rubella virus-stimulated lymphocytes from rubella-seropositive donors produced in the culture medium cytotoxic activity with preferential action against rubella-infected over uninfected target cells. The ability of lymphocytes to produce the cytotoxic activity upon stimulation by rubella virus correlated with the humoral rubella-immunity status, i.e. no such cytotoxic activity developed in the supernatants of lymphocyte cultures of rubella-seronegative donors. Stimulation of lymphocytes from seropositive donors by rubella virus was also detected by thymidine incorporation, but the correlation of lymphocyte responsiveness to the humoral rubella antibody status was not so clear as in the cytotoxicity assay. Conversion of lymphocytes from unresponsive to responsive to rubella virus following natural rubella infection and after rubella vaccination was demonstrated using both methods. Following vaccination rubella-specific cell-mediated immunity first became demonstrable at 14 days. The responsiveness of lymphocytes to phytohaemagglutinin (PHA) after rubella vaccination was followed by studying thymidine uptake and the ability of lymphocytes to produce lymphootoxin. By both tests marked suppression of PHA response occurred at days 3 and 7 after vaccination.", "contents": "Cell-mediated immunity in rubella assayed by cytotoxicity of supernatants from rubella virus-stimulated human lymphocyte cultures. Rubella virus-stimulated lymphocytes from rubella-seropositive donors produced in the culture medium cytotoxic activity with preferential action against rubella-infected over uninfected target cells. The ability of lymphocytes to produce the cytotoxic activity upon stimulation by rubella virus correlated with the humoral rubella-immunity status, i.e. no such cytotoxic activity developed in the supernatants of lymphocyte cultures of rubella-seronegative donors. Stimulation of lymphocytes from seropositive donors by rubella virus was also detected by thymidine incorporation, but the correlation of lymphocyte responsiveness to the humoral rubella antibody status was not so clear as in the cytotoxicity assay. Conversion of lymphocytes from unresponsive to responsive to rubella virus following natural rubella infection and after rubella vaccination was demonstrated using both methods. Following vaccination rubella-specific cell-mediated immunity first became demonstrable at 14 days. The responsiveness of lymphocytes to phytohaemagglutinin (PHA) after rubella vaccination was followed by studying thymidine uptake and the ability of lymphocytes to produce lymphootoxin. By both tests marked suppression of PHA response occurred at days 3 and 7 after vaccination."}
{"id": "PMID:1081926", "title": "In vitro activation of human T and B lymphocytes by pokeweed mitogen.", "content": "In previous in vitro studies the DNA synthesis in human blood lymphocytes induced by low concentration of PWM correlated with the percentage of bone marrow-derived (B) lymphocytes in cell suspensions. No correlation with lymphocyte subpopulations was noted when lymphocytes were activated by high concentrations of PWM. In this paper the hypothesis that low concentration of PWM mainly activates B lymphocytes was tested by measuring the [14C]thymidine incorporation into purified T or B lymphocytes from healthy donors. B lymphocytes were purified to 90--95% by buoyant density centrifugation of T lymphocytes rosetted with sheep red blood cells. T lymphocytes were enriched by passage of lymphocytes through an IgG-anti-IgG-coated column. Low concentration of PWM-stimulated B lymphocytes but not T lymphocytes, while high concentrations of the stimulant activated both cell types. It was also noted that the B- but not the T-lymphocyte fraction contained cells which synthesized DNA in the absence of PWM.", "contents": "In vitro activation of human T and B lymphocytes by pokeweed mitogen. In previous in vitro studies the DNA synthesis in human blood lymphocytes induced by low concentration of PWM correlated with the percentage of bone marrow-derived (B) lymphocytes in cell suspensions. No correlation with lymphocyte subpopulations was noted when lymphocytes were activated by high concentrations of PWM. In this paper the hypothesis that low concentration of PWM mainly activates B lymphocytes was tested by measuring the [14C]thymidine incorporation into purified T or B lymphocytes from healthy donors. B lymphocytes were purified to 90--95% by buoyant density centrifugation of T lymphocytes rosetted with sheep red blood cells. T lymphocytes were enriched by passage of lymphocytes through an IgG-anti-IgG-coated column. Low concentration of PWM-stimulated B lymphocytes but not T lymphocytes, while high concentrations of the stimulant activated both cell types. It was also noted that the B- but not the T-lymphocyte fraction contained cells which synthesized DNA in the absence of PWM."}
{"id": "PMID:1081927", "title": "Specific concentration of antilymphocyte antibodies in the serum cryoprecipitates of patients with systemic lupus erythematosus.", "content": "Antibodies to surface determinants of human lymphocytes, recognized both by cytotoxicity of fluorescent antibody analysis, were shown to be specifically enriched over the serum levels in cryoprecipitates from patients with systemic lupus erythematosus (SLE). The antilymphocyte antibody was shown to be cold reactive and was exclusively IgM. It was distinct from IgM anti-IgG, which was also variably concentrated in the cryoprecipitates. The question whether the antilymphocyte antibodies appear in the cryoprecipitates as complexes because of interaction with surface membrane antigens, or simply because of cold reactive properties, remains to be determined. The possible clinical relevance of the cryoprecipitation of these antibodies in systemic lupus erythematosus is discussed.", "contents": "Specific concentration of antilymphocyte antibodies in the serum cryoprecipitates of patients with systemic lupus erythematosus. Antibodies to surface determinants of human lymphocytes, recognized both by cytotoxicity of fluorescent antibody analysis, were shown to be specifically enriched over the serum levels in cryoprecipitates from patients with systemic lupus erythematosus (SLE). The antilymphocyte antibody was shown to be cold reactive and was exclusively IgM. It was distinct from IgM anti-IgG, which was also variably concentrated in the cryoprecipitates. The question whether the antilymphocyte antibodies appear in the cryoprecipitates as complexes because of interaction with surface membrane antigens, or simply because of cold reactive properties, remains to be determined. The possible clinical relevance of the cryoprecipitation of these antibodies in systemic lupus erythematosus is discussed."}
{"id": "PMID:1081928", "title": "Autoantibodies in patients with carcinoma of the breast. Correlation with prognosis.", "content": "Sera from a hundred patients with operable carcinoma of the breast and seventy-five age-matched controls were examined for antinuclear, smooth muscle, glomerular and mitochondrial antibodies by indirect immunofluorescence technique. Antinuclear and smooth muscle antibodies were found to be more frequent in cancer patients than in controls. The incidence of autoantibodies at the time the diagnosis was established was higher in patients who developed local recurrences or distant metastases within 2 years than in patients free from recurrence.", "contents": "Autoantibodies in patients with carcinoma of the breast. Correlation with prognosis. Sera from a hundred patients with operable carcinoma of the breast and seventy-five age-matched controls were examined for antinuclear, smooth muscle, glomerular and mitochondrial antibodies by indirect immunofluorescence technique. Antinuclear and smooth muscle antibodies were found to be more frequent in cancer patients than in controls. The incidence of autoantibodies at the time the diagnosis was established was higher in patients who developed local recurrences or distant metastases within 2 years than in patients free from recurrence."}
{"id": "PMID:1081929", "title": "In vitro differentiation of human marrow cells into T lymphocytes by thymic extracts using the rosette technique.", "content": "The manner by which human and calf thymic extracts induce precursor cells of human marrow to differentiate in vitro into T lymphocytes has been studied using as a T-cell marker the spontaneous rosette formation technique of human T lymphocytes with sheep erythrocytes (E rosette). These findings confirm previous observations made in the study of the same process using a different T-cell marker, specific antigenicity recognizable by a heterologous anti-human T-cell serum in a microcytotoxicity test. The number of cells revealing evidence of differentiation demonstrated by the E rosette formation technique is smaller than that obtained with the anti-human T-cell serum, indicating perhaps that a different stage of maturation of T lymphocytes is recognized by the antiserum from the one detected by spontaneous rosette formation. Based on the effects of specific inhibitors of nucleic acids and protein synthesis, it can be concluded that these thymic extracts ultimately act by influences exerted in the cell nucleus and that RNA and protein synthesis are required for the differentiation of precursor cells into T lymphocytes induced by thymic extracts. In addition, continued protein synthesis appears to be required for maintenance of receptors for sheep erythrocytes on the cell surface.", "contents": "In vitro differentiation of human marrow cells into T lymphocytes by thymic extracts using the rosette technique. The manner by which human and calf thymic extracts induce precursor cells of human marrow to differentiate in vitro into T lymphocytes has been studied using as a T-cell marker the spontaneous rosette formation technique of human T lymphocytes with sheep erythrocytes (E rosette). These findings confirm previous observations made in the study of the same process using a different T-cell marker, specific antigenicity recognizable by a heterologous anti-human T-cell serum in a microcytotoxicity test. The number of cells revealing evidence of differentiation demonstrated by the E rosette formation technique is smaller than that obtained with the anti-human T-cell serum, indicating perhaps that a different stage of maturation of T lymphocytes is recognized by the antiserum from the one detected by spontaneous rosette formation. Based on the effects of specific inhibitors of nucleic acids and protein synthesis, it can be concluded that these thymic extracts ultimately act by influences exerted in the cell nucleus and that RNA and protein synthesis are required for the differentiation of precursor cells into T lymphocytes induced by thymic extracts. In addition, continued protein synthesis appears to be required for maintenance of receptors for sheep erythrocytes on the cell surface."}
{"id": "PMID:1081930", "title": "The relationship of autoantibodies to depression of cell-mediated immunity in infectious mononucleosis.", "content": "It has been postulated that autoantibody formation occurs as a consequence of a depression of function of certain thymus-derived lymphocytes (T cells). We have examined cell-mediated immunity, a T-cell function, in infectious mononucleosis, a condition in which autoantibodies are known to develop. We have shown some evidence of depressed cell-mediated immunity in patients with infectious mononucleosis but have been unable to correlate this with autoantibody production. These results do not support the hypothesis that depression of T-cell function leads to autoantibody formation.", "contents": "The relationship of autoantibodies to depression of cell-mediated immunity in infectious mononucleosis. It has been postulated that autoantibody formation occurs as a consequence of a depression of function of certain thymus-derived lymphocytes (T cells). We have examined cell-mediated immunity, a T-cell function, in infectious mononucleosis, a condition in which autoantibodies are known to develop. We have shown some evidence of depressed cell-mediated immunity in patients with infectious mononucleosis but have been unable to correlate this with autoantibody production. These results do not support the hypothesis that depression of T-cell function leads to autoantibody formation."}
{"id": "PMID:1081931", "title": "Disparity in the production of lymphoblastogenesis inhibition factor by cultured human B and T lymphoid cell lines.", "content": "A comparative study of inhibitory effects of cell-free supernatants from cultured human B and T lymphoid cell lines on lymphocyte blastogenesis indicated that the inhibitory effect of supernatant from B lymphoid cells on lymphocyte blastogenesis was significantly higher than that of supernatant from T lymphoid cells or from non-lymphoid neoplastic cells. The inhibitory effect of supernatant was reversible and dose-related. The inhibitory effect gradually diminished with time when the supernatant from B lymphoid cells was added to the culture, 1-3 days after the beginning of cultures. The supernatant of human B lymphoid cells was also found to be highly active in affecting the mouse thymus cell response. The biological nature of this inhibitory factor has not been defined. Both B lymphoid cell lines used in the present study contained Epstein-Barr virus (EBV) genomes while the T-cell line and the non-lymphoid neoplastic cell lines were free of EBV genomes. Sensitivity of the supernatant of B lymphoid cells to u.v. irradiation and heat suggests the possibility that the EBV genomes released into the culture medium may be responsible for inhibition of lymphocyte blastogenesis; resistance of this supernatant to DNase suggests that the EBV genomes may be double-stranded DNA.", "contents": "Disparity in the production of lymphoblastogenesis inhibition factor by cultured human B and T lymphoid cell lines. A comparative study of inhibitory effects of cell-free supernatants from cultured human B and T lymphoid cell lines on lymphocyte blastogenesis indicated that the inhibitory effect of supernatant from B lymphoid cells on lymphocyte blastogenesis was significantly higher than that of supernatant from T lymphoid cells or from non-lymphoid neoplastic cells. The inhibitory effect of supernatant was reversible and dose-related. The inhibitory effect gradually diminished with time when the supernatant from B lymphoid cells was added to the culture, 1-3 days after the beginning of cultures. The supernatant of human B lymphoid cells was also found to be highly active in affecting the mouse thymus cell response. The biological nature of this inhibitory factor has not been defined. Both B lymphoid cell lines used in the present study contained Epstein-Barr virus (EBV) genomes while the T-cell line and the non-lymphoid neoplastic cell lines were free of EBV genomes. Sensitivity of the supernatant of B lymphoid cells to u.v. irradiation and heat suggests the possibility that the EBV genomes released into the culture medium may be responsible for inhibition of lymphocyte blastogenesis; resistance of this supernatant to DNase suggests that the EBV genomes may be double-stranded DNA."}
{"id": "PMID:1081932", "title": "Thyroiditis in T cell-depleted rats. Influence of strain, radiation dose, adjuvants and antilymphocyte serum.", "content": "Strain differences in susceptibility to the development of thyroiditis following adult thymectomy and repeated sublethal X-irradiation (4 X 200 rad) were investigated in the rat. Marked strain variation was noted, with AUG and PVG/c rats having the highest incidence and severity of lesion (100 and 80% respectively) whilst CAM rats had a low incidence (9%). WAG and LIS rats occupied an intermediate position. The incidence of autoantibodies to thyroglobulin correlated closely with the strain incidence of thyroiditis (r = 0-87). Fractionation of the sera from thymectomized and irradiated rats by gel filtration chromatography indicated that the bulk of the antibodies to thyroglobulin were of the IgG class. Freund's complete adjuvant, but not lipopolysaccharide, enhanced the rate of development of thyroiditis in thymectomized rats given a shortened series of irradiations. It was not found possible to induce thyroiditis in thymectomized rats by substituting antilymphocyte serum for irradiation as a T cell-depleting agent, despite the fact that the treated rats had markedly reduced responses to phytohaemagglutinin. A combination of thymectomy, three doses of 200 rad, and a development period of 8 weeks were found to be the optimum conditions for induction of severe thyroiditis together with high antibody titres to thyroglobulin. These findings add support for the role of thymus-derived cells in the regulation of autoimmune responses and further suggest that thymectomy, combined with a series of sublethal irradiations, causes a selective depletion of those T cells specifically involved in the suppression of autoimmune reactivity to thyroid components, whilst leaving autoreactive helper T cells unimpaired.", "contents": "Thyroiditis in T cell-depleted rats. Influence of strain, radiation dose, adjuvants and antilymphocyte serum. Strain differences in susceptibility to the development of thyroiditis following adult thymectomy and repeated sublethal X-irradiation (4 X 200 rad) were investigated in the rat. Marked strain variation was noted, with AUG and PVG/c rats having the highest incidence and severity of lesion (100 and 80% respectively) whilst CAM rats had a low incidence (9%). WAG and LIS rats occupied an intermediate position. The incidence of autoantibodies to thyroglobulin correlated closely with the strain incidence of thyroiditis (r = 0-87). Fractionation of the sera from thymectomized and irradiated rats by gel filtration chromatography indicated that the bulk of the antibodies to thyroglobulin were of the IgG class. Freund's complete adjuvant, but not lipopolysaccharide, enhanced the rate of development of thyroiditis in thymectomized rats given a shortened series of irradiations. It was not found possible to induce thyroiditis in thymectomized rats by substituting antilymphocyte serum for irradiation as a T cell-depleting agent, despite the fact that the treated rats had markedly reduced responses to phytohaemagglutinin. A combination of thymectomy, three doses of 200 rad, and a development period of 8 weeks were found to be the optimum conditions for induction of severe thyroiditis together with high antibody titres to thyroglobulin. These findings add support for the role of thymus-derived cells in the regulation of autoimmune responses and further suggest that thymectomy, combined with a series of sublethal irradiations, causes a selective depletion of those T cells specifically involved in the suppression of autoimmune reactivity to thyroid components, whilst leaving autoreactive helper T cells unimpaired."}
{"id": "PMID:1081933", "title": "Cytotoxic activity of lymphocytes from patients with Hodgkin's disease.", "content": "Blood lymphocytes from twenty-three untreated patients with Hodgkin's disease and twelve healthy controls were studied for their ability to lyse tissue culture cells (Chang cells) labelled with [51Cr]chromate. Lysis induced by patients' lymphocytes in the presence of PHA or rabbit IgG antibodies to Chang cells (ACS) was impaired in some, and higher than normal in others. ACS-induced lysis showed some corlation with the content of lymphocytes carrying receptors for human complement (CRL) in the effectory population. No correlation with immunoglobulin-bearing cells was noted. PHA-induced cytoxicity did not  correlate with lymphocyte subpopulations. The observations are consistent with the assumption that effector cells of antibody-induced lymphocyte-mediated cytotoxicity (K cells) may be present among CRL. The K-cell activity and PHA cytotoxicity by lymphocytes tended to decrease at high age, but impaired cytotoxity was also noted in young patients. A preliminary follow-up of patients 1-2 years after the beginning of treatment revealed almost abolished K-cell activity in four patients who died 6--13 months after testing. Patients in incomplete remission or with relapse after treatment had lower mean K-cell activity than those in complete remission. A similar, but less pronounced tendency was found for PHA-induced cytotoxicity. A prognostic role of impaired K-cell activity in Hodgkin's disease is suggested from these data, but requires confirmation in a larger clinical follow-up.", "contents": "Cytotoxic activity of lymphocytes from patients with Hodgkin's disease. Blood lymphocytes from twenty-three untreated patients with Hodgkin's disease and twelve healthy controls were studied for their ability to lyse tissue culture cells (Chang cells) labelled with [51Cr]chromate. Lysis induced by patients' lymphocytes in the presence of PHA or rabbit IgG antibodies to Chang cells (ACS) was impaired in some, and higher than normal in others. ACS-induced lysis showed some corlation with the content of lymphocytes carrying receptors for human complement (CRL) in the effectory population. No correlation with immunoglobulin-bearing cells was noted. PHA-induced cytoxicity did not  correlate with lymphocyte subpopulations. The observations are consistent with the assumption that effector cells of antibody-induced lymphocyte-mediated cytotoxicity (K cells) may be present among CRL. The K-cell activity and PHA cytotoxicity by lymphocytes tended to decrease at high age, but impaired cytotoxity was also noted in young patients. A preliminary follow-up of patients 1-2 years after the beginning of treatment revealed almost abolished K-cell activity in four patients who died 6--13 months after testing. Patients in incomplete remission or with relapse after treatment had lower mean K-cell activity than those in complete remission. A similar, but less pronounced tendency was found for PHA-induced cytotoxicity. A prognostic role of impaired K-cell activity in Hodgkin's disease is suggested from these data, but requires confirmation in a larger clinical follow-up."}
{"id": "PMID:1081934", "title": "Serum lymphocytotoxins in inflammatory bowel disease. Studies of frequency and specificity for lymphocyte subpopulations.", "content": "Serum cold-reactive lymphocytotoxin (LCT) was detected in twenty-two of fifty-six (40%) patients with inflammatory bowel disease (IBD). The frequency of LCT detection was similar in Crohn's disease and ulcerative colitis. Cytotoxicity testing against T or B cell-enriched peripheral blood lymphocytes from normal donors, together with absorption experiments, indicated that LCT in IBD was reactive against determinants on both cell subpopulations. Reactivity against T cells from patients with common variable immunodeficiency was significantly less than with normal donor T cells. LCT in IBD could not be related to prior allogeneic sensitization and its presence appeared to be unrelated to disease activity or drug therapy. No correlation was found between LCT and peripheral blood T- or B-cell numbers. The present findings suggest the need for further investigation of the role of infectious agents in the pathogenesis of IBD.", "contents": "Serum lymphocytotoxins in inflammatory bowel disease. Studies of frequency and specificity for lymphocyte subpopulations. Serum cold-reactive lymphocytotoxin (LCT) was detected in twenty-two of fifty-six (40%) patients with inflammatory bowel disease (IBD). The frequency of LCT detection was similar in Crohn's disease and ulcerative colitis. Cytotoxicity testing against T or B cell-enriched peripheral blood lymphocytes from normal donors, together with absorption experiments, indicated that LCT in IBD was reactive against determinants on both cell subpopulations. Reactivity against T cells from patients with common variable immunodeficiency was significantly less than with normal donor T cells. LCT in IBD could not be related to prior allogeneic sensitization and its presence appeared to be unrelated to disease activity or drug therapy. No correlation was found between LCT and peripheral blood T- or B-cell numbers. The present findings suggest the need for further investigation of the role of infectious agents in the pathogenesis of IBD."}
{"id": "PMID:1081935", "title": "Quantitation of immunoglobulin-bearing lymphocytes and the lymphocyte response to PHA in experimental pyelonephritis.", "content": "In these experiments the effect of experimental pyelonephritis on the distri-ution of B lymphocytes in the peripheral blood and lymphoid sites in the rat has been determined and the functional capacity of T cells during the course of infection has been investigated. The studies have shown that renal infection affects the distribution of lymphocytes and has a marked effect on the functional capacity of splenic T lymphocytes early in infection. Most of the lymphocytes forming the round cell infiltrate in the kidney have been identified as thymus-derived lymphocytes on their surface labelling characteristics. Evidence is presented to demonstrate the inability of T lymphocytes to function normally in the environment of the kdiney. It is suggested that ablation of cell-mediated immunity may be a factor contributing to the persistence of renal infection.", "contents": "Quantitation of immunoglobulin-bearing lymphocytes and the lymphocyte response to PHA in experimental pyelonephritis. In these experiments the effect of experimental pyelonephritis on the distri-ution of B lymphocytes in the peripheral blood and lymphoid sites in the rat has been determined and the functional capacity of T cells during the course of infection has been investigated. The studies have shown that renal infection affects the distribution of lymphocytes and has a marked effect on the functional capacity of splenic T lymphocytes early in infection. Most of the lymphocytes forming the round cell infiltrate in the kidney have been identified as thymus-derived lymphocytes on their surface labelling characteristics. Evidence is presented to demonstrate the inability of T lymphocytes to function normally in the environment of the kdiney. It is suggested that ablation of cell-mediated immunity may be a factor contributing to the persistence of renal infection."}
{"id": "PMID:1081939", "title": "[Emergency surgery in district hospital: massive hemorrhage after blunt abdominal injury].", "content": "In the management and prognosis of severe accident cases there are a number of conditions that occur before hospitalization which cannot be influenced, these are: no immediate medical care by a physician, delay in sending the patient to hospital, long journey time and alcoholic damage to the liver and CNS. Often, therefore, laparotomy for massive haemorrhage after abdominal injuries has to be done on a severely shocked patient with a \"zero\" BP. Diagnostic criteria in the evaluation of such cases are: the clinical picture, shock index, paracentesis (trial abdominal drainage) with additional laparoscopic examination and IVP. In a series of 45 laparotomies there were 25 single organ injuries; 19 multiple organ injuries and 1 negative laparotomy due to misinterpretation of the trial drainage. 22 splenectomies demonstrate the type of injury occurring in a country area. The good results of treatment despite the difficulties mentioned can, to some degree, be attributed to the technical improvement in vascular clips for large vessels. The Tantalum vascular clip offers the advantage of simple application, sure positioning and immediate arrest of haemorrhage thus reducing the shock phase; additional effects are the permanent indication of the bleeding site, the marking of anastomoses and resections and there being no foreign body reaction (pure Tantalum).", "contents": "[Emergency surgery in district hospital: massive hemorrhage after blunt abdominal injury]. In the management and prognosis of severe accident cases there are a number of conditions that occur before hospitalization which cannot be influenced, these are: no immediate medical care by a physician, delay in sending the patient to hospital, long journey time and alcoholic damage to the liver and CNS. Often, therefore, laparotomy for massive haemorrhage after abdominal injuries has to be done on a severely shocked patient with a \"zero\" BP. Diagnostic criteria in the evaluation of such cases are: the clinical picture, shock index, paracentesis (trial abdominal drainage) with additional laparoscopic examination and IVP. In a series of 45 laparotomies there were 25 single organ injuries; 19 multiple organ injuries and 1 negative laparotomy due to misinterpretation of the trial drainage. 22 splenectomies demonstrate the type of injury occurring in a country area. The good results of treatment despite the difficulties mentioned can, to some degree, be attributed to the technical improvement in vascular clips for large vessels. The Tantalum vascular clip offers the advantage of simple application, sure positioning and immediate arrest of haemorrhage thus reducing the shock phase; additional effects are the permanent indication of the bleeding site, the marking of anastomoses and resections and there being no foreign body reaction (pure Tantalum)."}
{"id": "PMID:1081945", "title": "Three distinct forms of nuclear poly(A) polymerase.", "content": "Poly(A) polymerase activities have been solubilized from rat liver nuclei and purified by chromatography on Bio-Gel A-1.5M, DEAE-Sephadex and CM-cellulose. Three distinct forms of nuclear poly(A) polymerase have been resolved by chromatography on CM-cellulose. According to their sequence of elution from CM-cellulose these enzyme activities have been termed A, B and C. Enzymes A and B are Mn2+ -dependent, enzyme C requires Mg2+. With the same chromatographic step on CM-cellulose the Mn+ -dependent poly(A) polymerase activities were separated from a Mn2+ -dependent enzyme system capable of synthesizing RNA-primed poly(U), poly(G) and poly(C). The effect of different nuclear and cytoplasmic RNA primers on the rate of poly(A) formation suggests enzyme A to be responsible for the elongation of preexisting poly (A) chains.  The phosphorylated derivated derivative of cordycepin, 3'-deoxyadenosine 5'-triphosphosphate (3'-dATP), which is known to inhibit nuclear poly(A) synthesis in vivo, also impairs poly(A) formation in vitro. It is shown that 3'-dATP very probably is not incorporated into poly(A)invitro, suggesting that 3'-dATP primarily affects the catalytic activities of the poly(A) polymerase species rather than directly blocking chain elongation.", "contents": "Three distinct forms of nuclear poly(A) polymerase. Poly(A) polymerase activities have been solubilized from rat liver nuclei and purified by chromatography on Bio-Gel A-1.5M, DEAE-Sephadex and CM-cellulose. Three distinct forms of nuclear poly(A) polymerase have been resolved by chromatography on CM-cellulose. According to their sequence of elution from CM-cellulose these enzyme activities have been termed A, B and C. Enzymes A and B are Mn2+ -dependent, enzyme C requires Mg2+. With the same chromatographic step on CM-cellulose the Mn+ -dependent poly(A) polymerase activities were separated from a Mn2+ -dependent enzyme system capable of synthesizing RNA-primed poly(U), poly(G) and poly(C). The effect of different nuclear and cytoplasmic RNA primers on the rate of poly(A) formation suggests enzyme A to be responsible for the elongation of preexisting poly (A) chains.  The phosphorylated derivated derivative of cordycepin, 3'-deoxyadenosine 5'-triphosphosphate (3'-dATP), which is known to inhibit nuclear poly(A) synthesis in vivo, also impairs poly(A) formation in vitro. It is shown that 3'-dATP very probably is not incorporated into poly(A)invitro, suggesting that 3'-dATP primarily affects the catalytic activities of the poly(A) polymerase species rather than directly blocking chain elongation."}
{"id": "PMID:1081946", "title": "5-Aminolevulinic-acid synthetases from Rhodopseudomonas spheroides Y. Comparison of the purification and properties of enzymes extracted from bacteria grown in different iron concentrations.", "content": "The two 5-aminolevulinic acid synthetases of Rhodopseudomonas spheroides Y. were extracted from cells grown in a 'low-iron' medium and purified. They have a specific activity 10-fold higher than the 'high-iron' enzymes described by us previously and have the same properties except that they do not contain any iron and have one free-SH group more per mole of enzyme (2 for E1; , for E2) Their inhibition by adenosine triphosphate and iron and their oxidation-reduction sensitivity are discussed in terms of light, oxygen and heme feed-back regulation of bacteriochlorophyll sunthesis.", "contents": "5-Aminolevulinic-acid synthetases from Rhodopseudomonas spheroides Y. Comparison of the purification and properties of enzymes extracted from bacteria grown in different iron concentrations. The two 5-aminolevulinic acid synthetases of Rhodopseudomonas spheroides Y. were extracted from cells grown in a 'low-iron' medium and purified. They have a specific activity 10-fold higher than the 'high-iron' enzymes described by us previously and have the same properties except that they do not contain any iron and have one free-SH group more per mole of enzyme (2 for E1; , for E2) Their inhibition by adenosine triphosphate and iron and their oxidation-reduction sensitivity are discussed in terms of light, oxygen and heme feed-back regulation of bacteriochlorophyll sunthesis."}
{"id": "PMID:1081949", "title": "Perception of linear horizontal self-motion induced by peripheral vision (linearvection) basic characteristics and visual-vestibular interactions.", "content": "The basic characteristics of the sensation of linear horizontal motion have been studied. Objective linear motion was induced by means of a moving cart. Visually induced linear motion perception (linearvection) was obtained by projection of moving images at the periphery of the visual field. Image velocity and luminance thresholds for the appearance of linearvection have been measured and are in the range of those for image motion detection (without sensation of self motion) by the visual system. Latencies of onset are around 1 sec and short term adaptation has been shown. The dynamic range of the visual analyser as judged by frequency analysis is lower than for the vestibular analyser. Conflicting situations in which visual cues contradict vestibular and other proprioceptive cues show, in the case of linearvection a dominance of vision which supports the idea of an essential although not independent role of vision in self motion perception.", "contents": "Perception of linear horizontal self-motion induced by peripheral vision (linearvection) basic characteristics and visual-vestibular interactions. The basic characteristics of the sensation of linear horizontal motion have been studied. Objective linear motion was induced by means of a moving cart. Visually induced linear motion perception (linearvection) was obtained by projection of moving images at the periphery of the visual field. Image velocity and luminance thresholds for the appearance of linearvection have been measured and are in the range of those for image motion detection (without sensation of self motion) by the visual system. Latencies of onset are around 1 sec and short term adaptation has been shown. The dynamic range of the visual analyser as judged by frequency analysis is lower than for the vestibular analyser. Conflicting situations in which visual cues contradict vestibular and other proprioceptive cues show, in the case of linearvection a dominance of vision which supports the idea of an essential although not independent role of vision in self motion perception."}
{"id": "PMID:1081951", "title": "Inhibition of respiration and of respiratory development by different doses of chloramphenicol in frog embryos (Rana pipiens).", "content": "High doses of chloramphenicol (4.5 and 9 mM) directly inhibited respiration in conjunction with an unusual cleavage anomaly (blastomere refusion) in embryos of the frog, Rana pipiens. Lower doses (2.1-3 mM) did not affect respiration or cleavage and, when added later, did not directly affect respiration or development at any tested stage. Continuous incubation in the latter doses, however, did eventually, by day 6 or 7, produce a parallel retardation of embryonic development and of respiratory development relative to controls, suggestive of impaired mitochondrial biogenesis. A lower continuous dose (0.9 mM) affected neither respiration nor development at any stage.", "contents": "Inhibition of respiration and of respiratory development by different doses of chloramphenicol in frog embryos (Rana pipiens). High doses of chloramphenicol (4.5 and 9 mM) directly inhibited respiration in conjunction with an unusual cleavage anomaly (blastomere refusion) in embryos of the frog, Rana pipiens. Lower doses (2.1-3 mM) did not affect respiration or cleavage and, when added later, did not directly affect respiration or development at any tested stage. Continuous incubation in the latter doses, however, did eventually, by day 6 or 7, produce a parallel retardation of embryonic development and of respiratory development relative to controls, suggestive of impaired mitochondrial biogenesis. A lower continuous dose (0.9 mM) affected neither respiration nor development at any stage."}
{"id": "PMID:1081952", "title": "Optical guiding by photoreceptor cells.", "content": "The potential for any morphological part of a photoreceptor cell to function as an optical waveguide is determined 1) by the dimensionless parameter V as shown by Snyder (IEEE Trans., Microwave Theory Tech. 17: 1133, 1969) and by Snyder, Pask and Mitchell (J. Opt. Soc. Am. 63: 59, 1973), and 2) by the nature of the optical excitation for that part of the cell. The effects of these two factors are considered for the myoids and outer segments of vertebrate photoreceptor cells and for the crystalline tracts and rhabdomers of invertebrate photoreceptor cells. Examples of both guiding and nonguiding myoids, outer segments, rhabdomers and tracts are cited and some consequences for vision are analyzed.", "contents": "Optical guiding by photoreceptor cells. The potential for any morphological part of a photoreceptor cell to function as an optical waveguide is determined 1) by the dimensionless parameter V as shown by Snyder (IEEE Trans., Microwave Theory Tech. 17: 1133, 1969) and by Snyder, Pask and Mitchell (J. Opt. Soc. Am. 63: 59, 1973), and 2) by the nature of the optical excitation for that part of the cell. The effects of these two factors are considered for the myoids and outer segments of vertebrate photoreceptor cells and for the crystalline tracts and rhabdomers of invertebrate photoreceptor cells. Examples of both guiding and nonguiding myoids, outer segments, rhabdomers and tracts are cited and some consequences for vision are analyzed."}
{"id": "PMID:1081955", "title": "The polycystic organ in senescent athymic nude mice and in euthymic mice.", "content": "The polycystic organ thought to be the thymic rudiment in \"athymic\" nude mice increased with age and continues its secretory activity even in senescent nude mice. Only the epithelial lining of the cysts undergoes pressure atrophy. Analogous polycystic structures can be found also in euthymic mice with or without the nu gene. A lymphoepithelial rudiment is found in the thymic area of nude mice; it may represent the true endodermal thymic rudiment and generate the T cells. In addition, a peculiar, sometimes enlarged and thymus-like node is present laterocaudally from the polycystic organ.", "contents": "The polycystic organ in senescent athymic nude mice and in euthymic mice. The polycystic organ thought to be the thymic rudiment in \"athymic\" nude mice increased with age and continues its secretory activity even in senescent nude mice. Only the epithelial lining of the cysts undergoes pressure atrophy. Analogous polycystic structures can be found also in euthymic mice with or without the nu gene. A lymphoepithelial rudiment is found in the thymic area of nude mice; it may represent the true endodermal thymic rudiment and generate the T cells. In addition, a peculiar, sometimes enlarged and thymus-like node is present laterocaudally from the polycystic organ."}
{"id": "PMID:1081961", "title": "Immunopathology of hepatitis B antigen positive and negative active chronic hepatitis.", "content": "Evidence is presented to suggest that an auto-immune response to a liver-specific lipoprotein antigen on the surface of hepatocytes is responsible for the progressive liver damage in active chronic hepatitis, and that in many cases infection with the hepatitis B virus is important in the initiation of the disease process. B cells appear to play a major role in the auto-immune response and may be activated by T cells reacting with viral antigens on the surface of infected hepatocytes. A postulated defect in humoral antibody production in HBAg-positive cases allows continued reinfection of the liver by the hepatitis B virus, persistence of a virus-directed T cell response and constant activation of B cells reacting with the liver-specific lipoprotein. In the HBAg -negative group the disease is also, in many cases initiated by an acute hepatitis, but here the auto-immune response continues after elimination of the virus because of a defect in the suppressor function of T cells which fail to turn off the autoreactive B cells. The histocompatibility antigen, HL-A8, may be a marker for such a defect in suppressor T cells - its increased frequency in active chronic hepatitis is restricted to the HBAg-negative cases. Finally, the hypothesis suggests how specific immunotherapy, either antibody to HBAg or Transfer Factor, could be given to correct the basic defects which lead to persistence of the auto-immune process.", "contents": "Immunopathology of hepatitis B antigen positive and negative active chronic hepatitis. Evidence is presented to suggest that an auto-immune response to a liver-specific lipoprotein antigen on the surface of hepatocytes is responsible for the progressive liver damage in active chronic hepatitis, and that in many cases infection with the hepatitis B virus is important in the initiation of the disease process. B cells appear to play a major role in the auto-immune response and may be activated by T cells reacting with viral antigens on the surface of infected hepatocytes. A postulated defect in humoral antibody production in HBAg-positive cases allows continued reinfection of the liver by the hepatitis B virus, persistence of a virus-directed T cell response and constant activation of B cells reacting with the liver-specific lipoprotein. In the HBAg -negative group the disease is also, in many cases initiated by an acute hepatitis, but here the auto-immune response continues after elimination of the virus because of a defect in the suppressor function of T cells which fail to turn off the autoreactive B cells. The histocompatibility antigen, HL-A8, may be a marker for such a defect in suppressor T cells - its increased frequency in active chronic hepatitis is restricted to the HBAg-negative cases. Finally, the hypothesis suggests how specific immunotherapy, either antibody to HBAg or Transfer Factor, could be given to correct the basic defects which lead to persistence of the auto-immune process."}
{"id": "PMID:1081963", "title": "Platelet hyperaggregation and increased plasma level of Von Willebrand factor in diabetics with retinopathy.", "content": "In 18 insulin-dependent diabetics (6 without retinopathy, 6 with proliferative retinopathy and 6 with proliferative retinopathy treated by hypophysectomy) matched for age and duration of diabetics, in vitro haemostasis was studied using ADP induced platelet aggregation, ristocetin induced platelet aggregation which allows von Willebrand factor (VIII VWF) assay, and determination of antihemophilic factor procoagulant activity (VII AHF). Using gel filtration-isolated platelets, the ADP induced hyperaggregation previously reported in diabetics with severe retinopathy untreated by hypophysectomy appeared to be related to a platelet and not a plasma factor; the normal results of thrombin induced aggregation suggests that the presumed abnormal platelet factor is related to the platelet plasma membrane. High level of plasma VII VWF was observed in diabetics with proliferative retinopathy while the VII AHF level was within normal limits.", "contents": "Platelet hyperaggregation and increased plasma level of Von Willebrand factor in diabetics with retinopathy. In 18 insulin-dependent diabetics (6 without retinopathy, 6 with proliferative retinopathy and 6 with proliferative retinopathy treated by hypophysectomy) matched for age and duration of diabetics, in vitro haemostasis was studied using ADP induced platelet aggregation, ristocetin induced platelet aggregation which allows von Willebrand factor (VIII VWF) assay, and determination of antihemophilic factor procoagulant activity (VII AHF). Using gel filtration-isolated platelets, the ADP induced hyperaggregation previously reported in diabetics with severe retinopathy untreated by hypophysectomy appeared to be related to a platelet and not a plasma factor; the normal results of thrombin induced aggregation suggests that the presumed abnormal platelet factor is related to the platelet plasma membrane. High level of plasma VII VWF was observed in diabetics with proliferative retinopathy while the VII AHF level was within normal limits."}
{"id": "PMID:1081966", "title": "The ultrastructure of hepatocytes in alpha-1-antitrypsin deficiency with the genotype Pi--.", "content": "The ultrastructural appearance of the endoplasmic reticulum of the hepatocytes was found to be normal in a 5-year-old girl with alpha-1-antitrypsin deficiency with the genotype Pi--. The liver ultrastructure of this variant is therefore different from that of alpha-1-antitrypsin deficiency with the genotype PiZZ in which aggregates of an abnormal, unsecreted alpha-1-antitrypsin accumulate in the endoplasmic reticulum of the hepatocytes. The normal appearance of the endoplasmic reticulum in alpha-1-antitrypsin deficiency with the genotype Pi-- is compatible with the hypothesis, in this variant, synthesis of alpha-1-antitrypsin is completely, or nearly completely, absent; an alternative hypothesis would be that an abnormal alpha-1-antitrypsin is produced by the liver and secreted into the plasma, but disappears rapidly from the plasma.", "contents": "The ultrastructure of hepatocytes in alpha-1-antitrypsin deficiency with the genotype Pi--. The ultrastructural appearance of the endoplasmic reticulum of the hepatocytes was found to be normal in a 5-year-old girl with alpha-1-antitrypsin deficiency with the genotype Pi--. The liver ultrastructure of this variant is therefore different from that of alpha-1-antitrypsin deficiency with the genotype PiZZ in which aggregates of an abnormal, unsecreted alpha-1-antitrypsin accumulate in the endoplasmic reticulum of the hepatocytes. The normal appearance of the endoplasmic reticulum in alpha-1-antitrypsin deficiency with the genotype Pi-- is compatible with the hypothesis, in this variant, synthesis of alpha-1-antitrypsin is completely, or nearly completely, absent; an alternative hypothesis would be that an abnormal alpha-1-antitrypsin is produced by the liver and secreted into the plasma, but disappears rapidly from the plasma."}
{"id": "PMID:1081964", "title": "[Wiscott-Aldrich syndrome. Description of a case].", "content": "The Wiscott-Aldrich syndrome with the classical symptomatology (eczema, thrombocytopenia and susceptibility to infections) has been described in a 3-month-old child. The family history, together with the evidence of an immunological deficiency involving both thymus-dependent lymphocyte and immunoglobulin-antibody systems have given important clues to diagnosis. Impairment of immunoglobulin homoeostasis and its relevance to the assessment of heterozygotes is discussed.", "contents": "[Wiscott-Aldrich syndrome. Description of a case]. The Wiscott-Aldrich syndrome with the classical symptomatology (eczema, thrombocytopenia and susceptibility to infections) has been described in a 3-month-old child. The family history, together with the evidence of an immunological deficiency involving both thymus-dependent lymphocyte and immunoglobulin-antibody systems have given important clues to diagnosis. Impairment of immunoglobulin homoeostasis and its relevance to the assessment of heterozygotes is discussed."}
{"id": "PMID:1081969", "title": "[Retrospective studies on stress ulcer].", "content": "The files of 100 stress-ulcer-patients (1960-1973) were carefully analyzed. These patients had usually complicated diseases before they developed stress ulcers. Melena was in 84 patients the initial symptom. 56 patients were treated by medical regimen, 34 patients underwent surgery. A partial gastrectomy was the procedure of choice except for poor-risk patients, who underwent vagotomy.", "contents": "[Retrospective studies on stress ulcer]. The files of 100 stress-ulcer-patients (1960-1973) were carefully analyzed. These patients had usually complicated diseases before they developed stress ulcers. Melena was in 84 patients the initial symptom. 56 patients were treated by medical regimen, 34 patients underwent surgery. A partial gastrectomy was the procedure of choice except for poor-risk patients, who underwent vagotomy."}
{"id": "PMID:1081972", "title": "Antileptospiral activity in lower-vertebrate sera.", "content": "Normal serum from the painted turtle (Chrysemys picta), the snapping turtle (Chelydra serpentina), and the frog (Rana pipiens) were found to possess bactericidal activity towards Leptospira. Leptospires from both the parasitic and biflexa complexes were killed by these sera at high dilutions. This pattern differs from that of mammalian serum, as generally only the biflexa complex leptospires are killed by normal mammalian serum. The activity in C. picta serum was characterized as being complement dependent and not mediated by basic proteins. Because comple-inactivated C. picta serum regained leptospiricidal activity after the addition of fresh rabbit serum, antibody is also likely to participate in the killing activity. Further support that C. picta serum contained leptospiral antibodies was found by the detection of serotype-specific agglutinins.", "contents": "Antileptospiral activity in lower-vertebrate sera. Normal serum from the painted turtle (Chrysemys picta), the snapping turtle (Chelydra serpentina), and the frog (Rana pipiens) were found to possess bactericidal activity towards Leptospira. Leptospires from both the parasitic and biflexa complexes were killed by these sera at high dilutions. This pattern differs from that of mammalian serum, as generally only the biflexa complex leptospires are killed by normal mammalian serum. The activity in C. picta serum was characterized as being complement dependent and not mediated by basic proteins. Because comple-inactivated C. picta serum regained leptospiricidal activity after the addition of fresh rabbit serum, antibody is also likely to participate in the killing activity. Further support that C. picta serum contained leptospiral antibodies was found by the detection of serotype-specific agglutinins."}
{"id": "PMID:1081973", "title": "In vitro bacteriostatic and bactericidal activities of 7 cephalosporin antibiotics on Haemophilus influenzae.", "content": "A total of 30 recently isolated strains of Haemophilus influenzae were tested for in vitro susceptibility to seven cephalosporins. In order of bacteriostatic effectiveness, the in vitro activity of the antibiotics studied was: cephapirin (median MIC = 0.78 mcg/ml), cephalothin (median MIC = 1.56 mcg/ml), cefazolin (median MIC: 1.56 mcg/ml), cephaloridine (median MIC = 3.12 mcg/ml), cefoxitin (median MIC = 3.12 mcg/ml), cephradine (median MIC = 12.5 mcg/ml) and cephalexin (median MIC = 25 mcg/ml). The bactericidal effectiveness of the seven compounds was studied against one of these strains. No drug was able to kill the selected strain in less than 6 hrs, even at 100 mcg per ml. Cefoxitin exhibited the best bactericidal effects, closely followed by cefazolin and cephapirin.", "contents": "In vitro bacteriostatic and bactericidal activities of 7 cephalosporin antibiotics on Haemophilus influenzae. A total of 30 recently isolated strains of Haemophilus influenzae were tested for in vitro susceptibility to seven cephalosporins. In order of bacteriostatic effectiveness, the in vitro activity of the antibiotics studied was: cephapirin (median MIC = 0.78 mcg/ml), cephalothin (median MIC = 1.56 mcg/ml), cefazolin (median MIC: 1.56 mcg/ml), cephaloridine (median MIC = 3.12 mcg/ml), cefoxitin (median MIC = 3.12 mcg/ml), cephradine (median MIC = 12.5 mcg/ml) and cephalexin (median MIC = 25 mcg/ml). The bactericidal effectiveness of the seven compounds was studied against one of these strains. No drug was able to kill the selected strain in less than 6 hrs, even at 100 mcg per ml. Cefoxitin exhibited the best bactericidal effects, closely followed by cefazolin and cephapirin."}
{"id": "PMID:1081974", "title": "On the radiation-induced aggregates of lactate dehydrogenase.", "content": "Radiolysis of lactate dehydrogenase under N2 leads to the formation of aggregates which are enzymatically inactive. These aggregates were isolated by gel filtration. Incubation with sodium dodecylsulphate followed by gel filtration made it obvious that these aggreates consist of protein fragments held togehter by hydrophobic and electrostatic interactions. Disulphide bridges were found to be unimportant for stabilizing the aggregates. All isolated protein fragments were smaller than the sub-units of lactate hydrogenase, indicating peptide-chain breaking as a major reaction in the radiolysis of proteins and in the inactivation process of enzymes.", "contents": "On the radiation-induced aggregates of lactate dehydrogenase. Radiolysis of lactate dehydrogenase under N2 leads to the formation of aggregates which are enzymatically inactive. These aggregates were isolated by gel filtration. Incubation with sodium dodecylsulphate followed by gel filtration made it obvious that these aggreates consist of protein fragments held togehter by hydrophobic and electrostatic interactions. Disulphide bridges were found to be unimportant for stabilizing the aggregates. All isolated protein fragments were smaller than the sub-units of lactate hydrogenase, indicating peptide-chain breaking as a major reaction in the radiolysis of proteins and in the inactivation process of enzymes."}
{"id": "PMID:1081975", "title": "Evidence for reproductive death of dividing cells in thymus and spleen following whole-body gamma-irradiation of mice.", "content": "Mice were given whole-body gamma-irradiation with 300 and 800 rad 1 hour or 1 day after the administration of 3H-thymidine. The time-course of the specific activity of DNA in thymus and spleen and the release of radioactivity into the fraction of DNA soluble in 0-14 N NaCl were determined for 28 hours after exposure. The results indicate that on average the loss of mitotically-active cells was delayed compared with the loss of a representative fraction of non-dividing cells. This suggested that different mechanisms might be implicated in the dying process of the dividing and non-dividing lymphoid cells. Using a dose-fractionation method, the total activity of DNA was determined in thymus and spleen at 24 hours after the first exposure and 25 hours after the in vivo labelling of DNA. A parallelism has been found between the results of this experiment and the recovery effect of the Elkind-Sutton type. It may be concluded that the dying process responsible for the loss of the dividing cells from thymus and spleen after irradiation probably involves reproductive death.", "contents": "Evidence for reproductive death of dividing cells in thymus and spleen following whole-body gamma-irradiation of mice. Mice were given whole-body gamma-irradiation with 300 and 800 rad 1 hour or 1 day after the administration of 3H-thymidine. The time-course of the specific activity of DNA in thymus and spleen and the release of radioactivity into the fraction of DNA soluble in 0-14 N NaCl were determined for 28 hours after exposure. The results indicate that on average the loss of mitotically-active cells was delayed compared with the loss of a representative fraction of non-dividing cells. This suggested that different mechanisms might be implicated in the dying process of the dividing and non-dividing lymphoid cells. Using a dose-fractionation method, the total activity of DNA was determined in thymus and spleen at 24 hours after the first exposure and 25 hours after the in vivo labelling of DNA. A parallelism has been found between the results of this experiment and the recovery effect of the Elkind-Sutton type. It may be concluded that the dying process responsible for the loss of the dividing cells from thymus and spleen after irradiation probably involves reproductive death."}
{"id": "PMID:1081976", "title": "The oxygen-enhancement ratio for reproductive death induced by 3H or 125I damage in mammalian cells.", "content": "The oxygen-enhancement ratio (o.e.r.) for 3H- and 125I-induced cell death at 4 degrees C was determined in cultured Chinese hamster cells. The o.e.r. for cell death induced by 3H-thymidine was 3-2, essentially the same value as that previously reported for X-ray induced cell death. For cell death induced by 125I-iododeoxyuridine (125IdUrd), the o.e.r. was less than 1-4. The lower o.e.r. for 125I-induced death was not due to the presence of the base analogue itself, since cells that had incorporated unlabelled IdUrd and were X-irradiated had an o.e.r. of 2-8 and cells that were inactivated by 3H-IdUrd damage at 4 degrees C had an o.e.r. even greater than 3. These results suggest that 125I-decay damage, like high-linear-energy-transfer radiation damage, is only slightly increased by the presence of oxygen.", "contents": "The oxygen-enhancement ratio for reproductive death induced by 3H or 125I damage in mammalian cells. The oxygen-enhancement ratio (o.e.r.) for 3H- and 125I-induced cell death at 4 degrees C was determined in cultured Chinese hamster cells. The o.e.r. for cell death induced by 3H-thymidine was 3-2, essentially the same value as that previously reported for X-ray induced cell death. For cell death induced by 125I-iododeoxyuridine (125IdUrd), the o.e.r. was less than 1-4. The lower o.e.r. for 125I-induced death was not due to the presence of the base analogue itself, since cells that had incorporated unlabelled IdUrd and were X-irradiated had an o.e.r. of 2-8 and cells that were inactivated by 3H-IdUrd damage at 4 degrees C had an o.e.r. even greater than 3. These results suggest that 125I-decay damage, like high-linear-energy-transfer radiation damage, is only slightly increased by the presence of oxygen."}
{"id": "PMID:1081977", "title": "DIP and DIP+2 as glutathione oxidants and radiation sensitizers in cultured Chinese hamster cells.", "content": "Two diamide analogues, diazene dicarboxylic acid bis (n'-methylpiperazide) or DIP, and its bis-N'-methyl iodide salt, or DIP + 2, were tested for their ability to penetrate cultured Chinese hamster cells and oxidize intracellular glutathione. DIP penetrated the cells at a reasonable rate at 18 degrees C, 160 nmoles being required to oxidize the endogenous glutathione of 2 X 10(6) cells, but it penetrated very slowly at 0 degrees C. DIP +2 did not effectively oxidize glutathione in Chinese hamster cells, possibly because it did not enter the cells. DIP became toxic after about 10 min of exposure, but its toxicity could be moderated by using anoxic conditions. DIP, but not DIP +2, sensitized anoxic Chinese hamster cells to X-radiation by a factor of 1-5, an effect that was due entirely to removal of the shoulder from the survival curve.", "contents": "DIP and DIP+2 as glutathione oxidants and radiation sensitizers in cultured Chinese hamster cells. Two diamide analogues, diazene dicarboxylic acid bis (n'-methylpiperazide) or DIP, and its bis-N'-methyl iodide salt, or DIP + 2, were tested for their ability to penetrate cultured Chinese hamster cells and oxidize intracellular glutathione. DIP penetrated the cells at a reasonable rate at 18 degrees C, 160 nmoles being required to oxidize the endogenous glutathione of 2 X 10(6) cells, but it penetrated very slowly at 0 degrees C. DIP +2 did not effectively oxidize glutathione in Chinese hamster cells, possibly because it did not enter the cells. DIP became toxic after about 10 min of exposure, but its toxicity could be moderated by using anoxic conditions. DIP, but not DIP +2, sensitized anoxic Chinese hamster cells to X-radiation by a factor of 1-5, an effect that was due entirely to removal of the shoulder from the survival curve."}
{"id": "PMID:1081978", "title": "Incorporation of tritiated thymidine of high specific activity into DNA of the mouse oocyte.", "content": "Radicals produced by X-irradiation at 77 K and at 300 K of cytosine. HCl crystals have been analysed by electron spin resonance spectroscopy. Four radicals have been identified: the anion radical of the cytosine molecule, the radical resulting from H-addition at position C6, the radical resulting from H-addition at position O2, and finally a radical resulting from addition of a Cl- to nitrogen N3. H\u00fcckel molecular orbital calculations are presented, which support the hypothesis according to which in unsaturated pyrimidines the site of hydrogenation or protonation depends on the state of the molecule.", "contents": "Incorporation of tritiated thymidine of high specific activity into DNA of the mouse oocyte. Radicals produced by X-irradiation at 77 K and at 300 K of cytosine. HCl crystals have been analysed by electron spin resonance spectroscopy. Four radicals have been identified: the anion radical of the cytosine molecule, the radical resulting from H-addition at position C6, the radical resulting from H-addition at position O2, and finally a radical resulting from addition of a Cl- to nitrogen N3. H\u00fcckel molecular orbital calculations are presented, which support the hypothesis according to which in unsaturated pyrimidines the site of hydrogenation or protonation depends on the state of the molecule."}
{"id": "PMID:1081979", "title": "Sensitization of thymine and uracil to ionizing radiation by p-nitroacetophenone.", "content": "p-Nitroacetophenone (PNAP) alters the radiation chemistry of thymine and uracil in de-aerated aqueous solution. In the case of thymine, it increases the yield of radiolysis products by a factor of almost two, and cis glycol of thymine is the major product. With uracil, the yield of the dimeric product, which is the major radiolysis product in de-aerated aqueous solution, is considerably reduced and the yield of cis glycol is increased. When the irradiation is carried out in N2O-saturated solution, PNAP enhances the yeild of glycol by a factor of two. The effects of cupric sulphate on the radiation chemistry of uracil are analogous to those of PNAP. A mechanism, involving a reaction between PNAP and the hydroxyl radical adduct of the pyrimidine proceeding through a one-step process, is postulated for the enhanced formation of glycol.", "contents": "Sensitization of thymine and uracil to ionizing radiation by p-nitroacetophenone. p-Nitroacetophenone (PNAP) alters the radiation chemistry of thymine and uracil in de-aerated aqueous solution. In the case of thymine, it increases the yield of radiolysis products by a factor of almost two, and cis glycol of thymine is the major product. With uracil, the yield of the dimeric product, which is the major radiolysis product in de-aerated aqueous solution, is considerably reduced and the yield of cis glycol is increased. When the irradiation is carried out in N2O-saturated solution, PNAP enhances the yeild of glycol by a factor of two. The effects of cupric sulphate on the radiation chemistry of uracil are analogous to those of PNAP. A mechanism, involving a reaction between PNAP and the hydroxyl radical adduct of the pyrimidine proceeding through a one-step process, is postulated for the enhanced formation of glycol."}
{"id": "PMID:1081982", "title": "The role of T-lymphocytes in the reactivation of presumed ocular histoplasmosis scars.", "content": "The surprising finding of viable lymphocytes in clinically inactive scars gives more credence to the immunological theory regarding reactivation of histoplasmosis scars. We feel that the T-lymphocyte theory can explain the mechanism by which an inactive scar becomes active upon reintroduction of the antigen. The clinical course of the disease as well as the response to corticosteroid therapy and photocoagulation are also consistent with this hypothesis.", "contents": "The role of T-lymphocytes in the reactivation of presumed ocular histoplasmosis scars. The surprising finding of viable lymphocytes in clinically inactive scars gives more credence to the immunological theory regarding reactivation of histoplasmosis scars. We feel that the T-lymphocyte theory can explain the mechanism by which an inactive scar becomes active upon reintroduction of the antigen. The clinical course of the disease as well as the response to corticosteroid therapy and photocoagulation are also consistent with this hypothesis."}
{"id": "PMID:1081983", "title": "Hereditary corneal dystrophy in the Manx cat: a preliminary report.", "content": "A progressive, apparently inherited corneal dystrophy is described in an inbred line of Manx cats. Initial changes in the cornea are seen at four months of age and characterized by anterior stromal edema. Progressive worsening of the condition produces severe bullous keratopathy with eventual breakdown of both epithelium and stroma. Light microscopic and ultrastructural studies in the advanced disease state revealed marked edema of the corneal stroma, disintegration of collagen material, and the formation of epithelial bullae. Ultrastructural evidence shows a normal endothelium to be present. The pathogenesis of this corneal dystrophy is not clear and further studies are underway.", "contents": "Hereditary corneal dystrophy in the Manx cat: a preliminary report. A progressive, apparently inherited corneal dystrophy is described in an inbred line of Manx cats. Initial changes in the cornea are seen at four months of age and characterized by anterior stromal edema. Progressive worsening of the condition produces severe bullous keratopathy with eventual breakdown of both epithelium and stroma. Light microscopic and ultrastructural studies in the advanced disease state revealed marked edema of the corneal stroma, disintegration of collagen material, and the formation of epithelial bullae. Ultrastructural evidence shows a normal endothelium to be present. The pathogenesis of this corneal dystrophy is not clear and further studies are underway."}
{"id": "PMID:1081984", "title": "Ampicillin-resistant Haemophilus influenzae pneumonia and empyema in an infant.", "content": "Ampicillin-resistant Haemophilus influenzae type b was isolated from the pleural fluid of a 15-month-old infant with pneumonia and empyema. The patient was cured when chloramphenicol treatment was substituted for ampicillin.", "contents": "Ampicillin-resistant Haemophilus influenzae pneumonia and empyema in an infant. Ampicillin-resistant Haemophilus influenzae type b was isolated from the pleural fluid of a 15-month-old infant with pneumonia and empyema. The patient was cured when chloramphenicol treatment was substituted for ampicillin."}
{"id": "PMID:1081985", "title": "[Indication and timing of conservative surgery of peripheral neurogenic vocal cord pareses (author's transl)].", "content": "1. In every case of a vocal cord paresis, its cause should be carefully sought. 2. \"Pseudopareses\" of the vocal cord can be excluded by electromygraphy. 3. In all cases of neurogneic vocal cord praeses and regardless of aetiology, immediate phoniatric training is indicated for acceleration of nerve regeneration as well as electrostimulation for the prevention of muscular atrophy and ankylosis of the cricoarytenoid joint. 4. If such therapy is neglected, the functional results of spontaneous regeneration, neurolysis and nerve plasty are doubtful. 5. If in cases of mechanical lesions of the recurrent nerve one decides to operate, electromyography of the larynx and a mobility test of the cricoarytenoid joints to be done first. 6. Compression or overstretching of the nerve should be followed by neurolysis after 5 months, unless the nerve has regenerated spontaneously. 7. When the recurrent nerve has had to be served it should be repaired by anastomosis as soon as possible. 8. Judging by our experience in regeneration of the laryngeal nerves, we feel that operations for opening or closure of the glottis are indicated only after 2 years.", "contents": "[Indication and timing of conservative surgery of peripheral neurogenic vocal cord pareses (author's transl)]. 1. In every case of a vocal cord paresis, its cause should be carefully sought. 2. \"Pseudopareses\" of the vocal cord can be excluded by electromygraphy. 3. In all cases of neurogneic vocal cord praeses and regardless of aetiology, immediate phoniatric training is indicated for acceleration of nerve regeneration as well as electrostimulation for the prevention of muscular atrophy and ankylosis of the cricoarytenoid joint. 4. If such therapy is neglected, the functional results of spontaneous regeneration, neurolysis and nerve plasty are doubtful. 5. If in cases of mechanical lesions of the recurrent nerve one decides to operate, electromyography of the larynx and a mobility test of the cricoarytenoid joints to be done first. 6. Compression or overstretching of the nerve should be followed by neurolysis after 5 months, unless the nerve has regenerated spontaneously. 7. When the recurrent nerve has had to be served it should be repaired by anastomosis as soon as possible. 8. Judging by our experience in regeneration of the laryngeal nerves, we feel that operations for opening or closure of the glottis are indicated only after 2 years."}
{"id": "PMID:1081986", "title": "Physical size of the donor locus and transmission of Haemophilus influenzae ampicillin resistance genes by deoxyribonucleic acid-mediated transformation.", "content": "The properties of donor deoxyribonucleic acid (DNA) from three clinical isolates and its ability to mediate the transformation of competent Rd strains to ampicillin resistance were examined. A quantitative technique for determining the resistance of individual Haemophilus influenzae cells to ampicillin was developed. When this technique was used, sensitive cells failed to tolerate levels of ampicillin greater than 0.1 to 0.2 mug/ml, whereas three resistant type b beta-lactamase-producing strains could form from the colonies in 1- to 3-mug/ml levels of the antibiotic. DNA extracted from the resistant strains elicited transformation of the auxotrophic genes in a multiply auxotrophic Rd strain. For two of the donors, transformation to ampicillin resistance occurred after the uptake of a single DNA molecule approximately 104-fold less frequently than transformation of auxotrophic loci and was not observed to occur at all with the third. The frequency of transformation to ampicillin resistance was two- to fivefold higher in strain BC200 (Okinaka and Barnhart, 1974), which was cured of a defective prophage. All three clinical ampicillin-resistant strains were poor recipients, but the presence of the ampicillin resistant genes in strain BC200 did not reduce its competence. Sucrose gradients of DNA from ampicillin-resistant transformants of BC200 and from the original ampicillin-resistant strains showed that: (i) all the DNA preparations had high molecular weights; (ii) donor activity for ampicillin resistance sedimented heterogeneously and in parallel with genome DNA up to the highest molecular weights observed (100 x 106 to 200 x 106); and (iii) genetic transformation of ampicillin resistance from strain BC200-amp90383 required the physical integrity of a linearly integrated segment of DNA having a molecular weight of about 25 x 106 to 30 x 106.", "contents": "Physical size of the donor locus and transmission of Haemophilus influenzae ampicillin resistance genes by deoxyribonucleic acid-mediated transformation. The properties of donor deoxyribonucleic acid (DNA) from three clinical isolates and its ability to mediate the transformation of competent Rd strains to ampicillin resistance were examined. A quantitative technique for determining the resistance of individual Haemophilus influenzae cells to ampicillin was developed. When this technique was used, sensitive cells failed to tolerate levels of ampicillin greater than 0.1 to 0.2 mug/ml, whereas three resistant type b beta-lactamase-producing strains could form from the colonies in 1- to 3-mug/ml levels of the antibiotic. DNA extracted from the resistant strains elicited transformation of the auxotrophic genes in a multiply auxotrophic Rd strain. For two of the donors, transformation to ampicillin resistance occurred after the uptake of a single DNA molecule approximately 104-fold less frequently than transformation of auxotrophic loci and was not observed to occur at all with the third. The frequency of transformation to ampicillin resistance was two- to fivefold higher in strain BC200 (Okinaka and Barnhart, 1974), which was cured of a defective prophage. All three clinical ampicillin-resistant strains were poor recipients, but the presence of the ampicillin resistant genes in strain BC200 did not reduce its competence. Sucrose gradients of DNA from ampicillin-resistant transformants of BC200 and from the original ampicillin-resistant strains showed that: (i) all the DNA preparations had high molecular weights; (ii) donor activity for ampicillin resistance sedimented heterogeneously and in parallel with genome DNA up to the highest molecular weights observed (100 x 106 to 200 x 106); and (iii) genetic transformation of ampicillin resistance from strain BC200-amp90383 required the physical integrity of a linearly integrated segment of DNA having a molecular weight of about 25 x 106 to 30 x 106."}
{"id": "PMID:1081987", "title": "Single-stranded regions in transforming deoxyribonucleic acid after uptake by competent Haemophilus influenzae.", "content": "About 15% of donor deoxyribonucleic acid (DNA) is single stranded immediately after uptake into competent Haemophilus influenzae wild-type cells, as judged by its sensitivity to S1 endonuclease. This amount decreases to 4 to 5% by 30 min after uptake. Mutants which are defective in the covalent association of recipient and donor DNA form little or no S1 endonuclease-sensitive donor. At 17 C donor DNA taken up by the wild type contains single-stranded regions although there is no observable association, either covalent or noncovalent. The single-stranded regions are at the ends of donor DNA molecules, as judged by the unchanged sedimentation velocity after S1 endonuclease digestion. The amount of single-stranded donor remains constant at 17 C for more than 60 min after uptake, suggesting that the decrease observed at 37 C is the result of association of single-stranded ends with single-stranded regions of recipient cell DNA. Three sequential steps necessary for the integration of donor DNA into recipient DNA are proposed: the synthesis of single-stranded regions in recipient DNA, the interaction of donor DNA with recipient DNA resulting in the production of single-stranded ends on donor DNA, and the stable pairing of homologous single-stranded regions.", "contents": "Single-stranded regions in transforming deoxyribonucleic acid after uptake by competent Haemophilus influenzae. About 15% of donor deoxyribonucleic acid (DNA) is single stranded immediately after uptake into competent Haemophilus influenzae wild-type cells, as judged by its sensitivity to S1 endonuclease. This amount decreases to 4 to 5% by 30 min after uptake. Mutants which are defective in the covalent association of recipient and donor DNA form little or no S1 endonuclease-sensitive donor. At 17 C donor DNA taken up by the wild type contains single-stranded regions although there is no observable association, either covalent or noncovalent. The single-stranded regions are at the ends of donor DNA molecules, as judged by the unchanged sedimentation velocity after S1 endonuclease digestion. The amount of single-stranded donor remains constant at 17 C for more than 60 min after uptake, suggesting that the decrease observed at 37 C is the result of association of single-stranded ends with single-stranded regions of recipient cell DNA. Three sequential steps necessary for the integration of donor DNA into recipient DNA are proposed: the synthesis of single-stranded regions in recipient DNA, the interaction of donor DNA with recipient DNA resulting in the production of single-stranded ends on donor DNA, and the stable pairing of homologous single-stranded regions."}
{"id": "PMID:1081990", "title": "Lipid A, the active part of bacterial endotoxins in inducing serum colony stimulating activity and proliferation of splenic granulocyte/macrophage progenitor cells.", "content": "An analysis of which component of lipopolysaccharides (LPS), the lipid or the polysaccharide (PS), is active in stimulating the murine granulopoietic system has been performed. LPS with different structures, isolated from different mutant strains of Salmonella and chemical degradation products of lipopolysaccharides have been used. Lipid A obtained by acid hydrolysys of the LPS and complexed to bovine serum albumin (BSA) (lipid A-BSA) was shown to be active in generating serum colony stimulating factor (CSF) and in increasing the splenic colony forming cells (CFC) levels, although it was less active than the parent LPS. The polysaccharide (PS) showed no significant activity at the concentrations used. LPS (glycolipids) from R mutants of Salmonella minnesota were active to the same extent as the LPS. The fact that even the most defective LPS from the R mutant R595 which contains lipid A and KDO only is a potent endotoxin, points unequivocally, to lipid A, as the active principle in stimulating the granulopoietic system.", "contents": "Lipid A, the active part of bacterial endotoxins in inducing serum colony stimulating activity and proliferation of splenic granulocyte/macrophage progenitor cells. An analysis of which component of lipopolysaccharides (LPS), the lipid or the polysaccharide (PS), is active in stimulating the murine granulopoietic system has been performed. LPS with different structures, isolated from different mutant strains of Salmonella and chemical degradation products of lipopolysaccharides have been used. Lipid A obtained by acid hydrolysys of the LPS and complexed to bovine serum albumin (BSA) (lipid A-BSA) was shown to be active in generating serum colony stimulating factor (CSF) and in increasing the splenic colony forming cells (CFC) levels, although it was less active than the parent LPS. The polysaccharide (PS) showed no significant activity at the concentrations used. LPS (glycolipids) from R mutants of Salmonella minnesota were active to the same extent as the LPS. The fact that even the most defective LPS from the R mutant R595 which contains lipid A and KDO only is a potent endotoxin, points unequivocally, to lipid A, as the active principle in stimulating the granulopoietic system."}
{"id": "PMID:1081988", "title": "Distribution of coronary artery disease in patients with isolate aortic valve disease.", "content": "Forty-four adult patients with isolated aortic valve disease underwent coronary arteriography. Seventy precent of patients required aortic valve replacement (AVR). Of 28 men who underwent AVR, 59 percent of those with severe aortic stenosis, 33 percent of those with severe aortic regurgitation and 50 percent of those with mixed aortic stenosis/aortic regurgitation had associated coronary artery disease. Thirty-six percent of men with aortic valve disease not requiring AVR had significant coronary artery disease (CAD). CAD was not found in the 5 female patients studied. In the patients with CAD, the left anterior descending artery was involved 84 percent of the time. Next in frequency were the right coronary artery, the proximal left circumflex artery, and the obtuse marginal artery. Multivessel disease was the rule. All but one patient with significant CAD had angina pectoris, but many patients with angina had normal coronary arteries. The frequent occurence of significant CAD in adult male patients with isolated aortic valve disease argues for the performance of selective coronary arteriography in all such patients in whom AVR is a consideration.", "contents": "Distribution of coronary artery disease in patients with isolate aortic valve disease. Forty-four adult patients with isolated aortic valve disease underwent coronary arteriography. Seventy precent of patients required aortic valve replacement (AVR). Of 28 men who underwent AVR, 59 percent of those with severe aortic stenosis, 33 percent of those with severe aortic regurgitation and 50 percent of those with mixed aortic stenosis/aortic regurgitation had associated coronary artery disease. Thirty-six percent of men with aortic valve disease not requiring AVR had significant coronary artery disease (CAD). CAD was not found in the 5 female patients studied. In the patients with CAD, the left anterior descending artery was involved 84 percent of the time. Next in frequency were the right coronary artery, the proximal left circumflex artery, and the obtuse marginal artery. Multivessel disease was the rule. All but one patient with significant CAD had angina pectoris, but many patients with angina had normal coronary arteries. The frequent occurence of significant CAD in adult male patients with isolated aortic valve disease argues for the performance of selective coronary arteriography in all such patients in whom AVR is a consideration."}
{"id": "PMID:1081991", "title": "Platelet membrane glycoproteins implicated in ristocetin-induced aggregation. Studies of the proteins on platelets from patients with Bernard-Soulier syndrome and von Willebrand's disease.", "content": "The antibiotic ristocetin only aggregates platelets in the presence of plasma von Willebrand factor. Platelets from patients with Bernard-Soulier syndrome do not aggregate upon addition of ristocetin although, in contrast to von Willebrand's disease, plasma levels of factor VIII complex (factor VIII clotting activity, von Willebrand factor activity, and von Willebrand antigen) are normal. The membrane surface of normal platelets was modified and compared to the surface of platelets from a patient with Bernard-Soulier syndrome in an attempt to identify the receptor involved in von Willebrand factor-ristocetin-induced aggregation. After the incubation of washed normal platelets with a preparation of ristocetin previously shown to contain a proteolytic contaminant, the aggregation response is significantly decreased on addition or normal plasma. Analaysis by gel electrophoresis of such platelets when stained for carbohydrate revealed a decrease in the relative amounts of membrane glycopro-eins. Chymotrypsin-treated normal platelets had less membrane glycoproteins in addition to giving a reduced aggregation response in ristocetin-induced aggregation. Staining of gels for protein and carbohydrate indicated that there was an extensive change in the surface of Bernard-Soulier platelets, whereas those from patients with von Willebrand's disease appeared the same as normal. Platelets from patients were labeled by the lactoperoxidase iodination technique. Not only was the relative intensity of staining of platelet-specific proteins and glycoproteins changed in Bernard-Soulier platelets, but the iodination of the glycoproteins on the membrane surface relative to other membrane constituents was lower. In contrast, platelets from patients with von Willebrand's disease showed a normal exposure of membrane components. These data suggest therefore that membrane glycoproteins may play a functional role in ristocetin-induced aggregation.", "contents": "Platelet membrane glycoproteins implicated in ristocetin-induced aggregation. Studies of the proteins on platelets from patients with Bernard-Soulier syndrome and von Willebrand's disease. The antibiotic ristocetin only aggregates platelets in the presence of plasma von Willebrand factor. Platelets from patients with Bernard-Soulier syndrome do not aggregate upon addition of ristocetin although, in contrast to von Willebrand's disease, plasma levels of factor VIII complex (factor VIII clotting activity, von Willebrand factor activity, and von Willebrand antigen) are normal. The membrane surface of normal platelets was modified and compared to the surface of platelets from a patient with Bernard-Soulier syndrome in an attempt to identify the receptor involved in von Willebrand factor-ristocetin-induced aggregation. After the incubation of washed normal platelets with a preparation of ristocetin previously shown to contain a proteolytic contaminant, the aggregation response is significantly decreased on addition or normal plasma. Analaysis by gel electrophoresis of such platelets when stained for carbohydrate revealed a decrease in the relative amounts of membrane glycopro-eins. Chymotrypsin-treated normal platelets had less membrane glycoproteins in addition to giving a reduced aggregation response in ristocetin-induced aggregation. Staining of gels for protein and carbohydrate indicated that there was an extensive change in the surface of Bernard-Soulier platelets, whereas those from patients with von Willebrand's disease appeared the same as normal. Platelets from patients were labeled by the lactoperoxidase iodination technique. Not only was the relative intensity of staining of platelet-specific proteins and glycoproteins changed in Bernard-Soulier platelets, but the iodination of the glycoproteins on the membrane surface relative to other membrane constituents was lower. In contrast, platelets from patients with von Willebrand's disease showed a normal exposure of membrane components. These data suggest therefore that membrane glycoproteins may play a functional role in ristocetin-induced aggregation."}
{"id": "PMID:1081992", "title": "Immunoglobulin-bearing and complement-receptor lymphocytes constitute the same population in human peripheral blood.", "content": "Complement-receptor lymphocytes have generally been considered to be a subpopulation of bone-marrow derived (B) lymphocytes. However, the present studies show that essentially all cells with integral surface immunoglobulin from normal human peripheral blood bear receptors for the third component of complement. Moreover, after removal of phagocytes, all cells with complement receptors bear surface Ig. Thus, circulating B cells and complement-receptor lymphocytes are the same population.", "contents": "Immunoglobulin-bearing and complement-receptor lymphocytes constitute the same population in human peripheral blood. Complement-receptor lymphocytes have generally been considered to be a subpopulation of bone-marrow derived (B) lymphocytes. However, the present studies show that essentially all cells with integral surface immunoglobulin from normal human peripheral blood bear receptors for the third component of complement. Moreover, after removal of phagocytes, all cells with complement receptors bear surface Ig. Thus, circulating B cells and complement-receptor lymphocytes are the same population."}
{"id": "PMID:1081996", "title": "Absolute macrophage dependency of T lymphocyte activation by mitogens.", "content": "A T lymphocyte subpopulation that contains only 0.3% macrophages and less than 2% B lymphocytes has been prepared from guinea pig lymph node cells by the use of two different types of adherence columns. This subpopulation does not porliferate in response to the mitogens Con A or PHA unless additional macrophages are added. The means by which macrophages restore T cell responsiveness to PHA has been investigated. Marcophages appear to function via two different distinct mechanisms in this experimental situation. The first mechanism involves the binding of PHA to the macrophage followed by the \"presentation\" of the mitogen to the T lymphocyte in a manner that induces cell activation. This presentation function requires that the macrophage be viable and metabolically active. The second mechanism by which macrophages function is by the elaboration of a soluble factor or factors. The presence of these factors has been reliably and reproducibly demonstrated by using a double-chambered, Marbrook-type tissue culture vessel. This soluble factor can induce activation of T lympohcytes with surface bound PHA in the apparent absence of any form of macrophage presentation. In contrast, the function of this factor is clearly distinct from that of the reducing agent, 2-mercaptoethanol, (2-ME) since 2-ME does not enable this T cell subpopulation to be activated by mitogens. On the basis of these observations, we propose that two distinct signals are required to activate this T lymphocyte subpopulation. One signal is delivered by the interaction of the mitogen with the T cell surface, and the second signal is delivered by a soluble factor(s) produced by macrophages. Whether all types of T lymphocytes require two signals to be activated, remains to be established.", "contents": "Absolute macrophage dependency of T lymphocyte activation by mitogens. A T lymphocyte subpopulation that contains only 0.3% macrophages and less than 2% B lymphocytes has been prepared from guinea pig lymph node cells by the use of two different types of adherence columns. This subpopulation does not porliferate in response to the mitogens Con A or PHA unless additional macrophages are added. The means by which macrophages restore T cell responsiveness to PHA has been investigated. Marcophages appear to function via two different distinct mechanisms in this experimental situation. The first mechanism involves the binding of PHA to the macrophage followed by the \"presentation\" of the mitogen to the T lymphocyte in a manner that induces cell activation. This presentation function requires that the macrophage be viable and metabolically active. The second mechanism by which macrophages function is by the elaboration of a soluble factor or factors. The presence of these factors has been reliably and reproducibly demonstrated by using a double-chambered, Marbrook-type tissue culture vessel. This soluble factor can induce activation of T lympohcytes with surface bound PHA in the apparent absence of any form of macrophage presentation. In contrast, the function of this factor is clearly distinct from that of the reducing agent, 2-mercaptoethanol, (2-ME) since 2-ME does not enable this T cell subpopulation to be activated by mitogens. On the basis of these observations, we propose that two distinct signals are required to activate this T lymphocyte subpopulation. One signal is delivered by the interaction of the mitogen with the T cell surface, and the second signal is delivered by a soluble factor(s) produced by macrophages. Whether all types of T lymphocytes require two signals to be activated, remains to be established."}
{"id": "PMID:1081997", "title": "Studies on the mechanism of lymphocyte-mediated cytolysis. VI. A reappraisal of the requirement for protein synthesis during T cell-mediated lysis.", "content": "The role of protein synthesis in the mechanism of T cell-mediated cytolysis has been re-investigated. Cytolytically active (C57BL/L anti-DBA/2) spleen cells treated with pactamycin (10-7 M to 10-6 M) exhibited suppressed protein synthesis (100 +/- 5%), but unimpeded lytic activity. Drug-treated effector cells, incubated for prolonged (up to 24 hr) periods of time in the presence and absence of antigen, showed no siginificant diminution of lytic activity although the incorporation of 3H-leucine into protein was totally ablated. Studies with emetine, another irreversible inhibitor of protein synthesis, gave identical results. These findings are difficult to reconcile with the hypothesis that effector T cells lysis via a soluble protein mediator.", "contents": "Studies on the mechanism of lymphocyte-mediated cytolysis. VI. A reappraisal of the requirement for protein synthesis during T cell-mediated lysis. The role of protein synthesis in the mechanism of T cell-mediated cytolysis has been re-investigated. Cytolytically active (C57BL/L anti-DBA/2) spleen cells treated with pactamycin (10-7 M to 10-6 M) exhibited suppressed protein synthesis (100 +/- 5%), but unimpeded lytic activity. Drug-treated effector cells, incubated for prolonged (up to 24 hr) periods of time in the presence and absence of antigen, showed no siginificant diminution of lytic activity although the incorporation of 3H-leucine into protein was totally ablated. Studies with emetine, another irreversible inhibitor of protein synthesis, gave identical results. These findings are difficult to reconcile with the hypothesis that effector T cells lysis via a soluble protein mediator."}
{"id": "PMID:1081998", "title": "Thymosin restores T cell function and reduces the incidence of amyloid disease in casein-treated mice.", "content": "Evidence is presented that T cell impairment appears to be specifically related to the pathogenesis of experimental amyloidosis. This conclusion is based on the finding that thymosin administration improves T cell function as measured by mitogen stimulation of spleen cell suspension and at the same time reduces the incidence and severity of amyloid disease in casein-treated mice.", "contents": "Thymosin restores T cell function and reduces the incidence of amyloid disease in casein-treated mice. Evidence is presented that T cell impairment appears to be specifically related to the pathogenesis of experimental amyloidosis. This conclusion is based on the finding that thymosin administration improves T cell function as measured by mitogen stimulation of spleen cell suspension and at the same time reduces the incidence and severity of amyloid disease in casein-treated mice."}
{"id": "PMID:1081999", "title": "Experimental autoimmune orchitis: in vitro induction of an autoimmune disease.", "content": "Experimental autoimmune orchitis (EAO) can be induced in vitro. Normal lymph node lymphocytes cultured with autologous dissociated testis cells form rosette-like aggregates and later undergo blast transformation and proliferation. These stimulated lymphocytes cause in vivo EAO lesions, when injectd into syngeneic recipients. Moreover, their autoimmune reactivity can be monitored by an in vitro cytostasis assay. Density gradient analysis of the early lymphocyte-testis cultures reveals that the autoimmune reactive lymphocytes are enriched in the rosette populations. It therefore appears that testicular self-antigens are recognized by clonally preformed autologous lymphocytes.", "contents": "Experimental autoimmune orchitis: in vitro induction of an autoimmune disease. Experimental autoimmune orchitis (EAO) can be induced in vitro. Normal lymph node lymphocytes cultured with autologous dissociated testis cells form rosette-like aggregates and later undergo blast transformation and proliferation. These stimulated lymphocytes cause in vivo EAO lesions, when injectd into syngeneic recipients. Moreover, their autoimmune reactivity can be monitored by an in vitro cytostasis assay. Density gradient analysis of the early lymphocyte-testis cultures reveals that the autoimmune reactive lymphocytes are enriched in the rosette populations. It therefore appears that testicular self-antigens are recognized by clonally preformed autologous lymphocytes."}
{"id": "PMID:1082000", "title": "Cellular receptors for lymphotoxin: correlation of binding and cytotoxicity in sensitive and resistant target cells.", "content": "The binding of radioactively labeled lymphotoxin (LT) to both lymphotoxin-sensitive and -resistant cell clones was examined. The sensitive clone had a low- capacity, high-affinity (\"specific\") binding component, the curve of which closely followed the cytotoxicity curve of the lymphocyte mediator. The capacity of this binding component was calculated to be about 600 molecules of LT/cell. In addition, there was a low-affinity, high-capacity (\"nonspecific\") binding component. In striking contrast, the high-affinity, low-capacity (\"specific\") component was absent or greatly diminished from the resistant clone, whereas the low-affinity, high-capacity (\"nonspecific\") component was present at a similar level as in the sensitive cells. These binding characteristics closely resemble those observed by us and other investigators working with a variety of steroid hormones in steroid-sensitive and- resistant cell lines.", "contents": "Cellular receptors for lymphotoxin: correlation of binding and cytotoxicity in sensitive and resistant target cells. The binding of radioactively labeled lymphotoxin (LT) to both lymphotoxin-sensitive and -resistant cell clones was examined. The sensitive clone had a low- capacity, high-affinity (\"specific\") binding component, the curve of which closely followed the cytotoxicity curve of the lymphocyte mediator. The capacity of this binding component was calculated to be about 600 molecules of LT/cell. In addition, there was a low-affinity, high-capacity (\"nonspecific\") binding component. In striking contrast, the high-affinity, low-capacity (\"specific\") component was absent or greatly diminished from the resistant clone, whereas the low-affinity, high-capacity (\"nonspecific\") component was present at a similar level as in the sensitive cells. These binding characteristics closely resemble those observed by us and other investigators working with a variety of steroid hormones in steroid-sensitive and- resistant cell lines."}
{"id": "PMID:1082001", "title": "A microculture method for the generation of primary immune responses in vitro.", "content": "A microculture method for the generation and study of the primary immune response of murine spleen cells to defined antigens in vitro is described. Many of the variable parameters which occur in culture systems have been studied in an attempt to define the optimal culture conditions for this system. Cultures of 10(6) CBA spleen cells consistently produced an immune response of 300-600 hapten-specific plaque-forming cells after 3 days of incubation with the T cell-independent antigens DNP-POL and NIP-POL. Cultures were set up in Microtest II tissue culture plates in a volume of 0.2 ml of medium containing 10(-4) M 2-mercaptoethanol. The system described has the advantages of being highly efficient and reproducible and utilises small amounts of cells, medium and antigen. It provides a simple, economic and reliable approach for the systematic study of the immune response in vitro.", "contents": "A microculture method for the generation of primary immune responses in vitro. A microculture method for the generation and study of the primary immune response of murine spleen cells to defined antigens in vitro is described. Many of the variable parameters which occur in culture systems have been studied in an attempt to define the optimal culture conditions for this system. Cultures of 10(6) CBA spleen cells consistently produced an immune response of 300-600 hapten-specific plaque-forming cells after 3 days of incubation with the T cell-independent antigens DNP-POL and NIP-POL. Cultures were set up in Microtest II tissue culture plates in a volume of 0.2 ml of medium containing 10(-4) M 2-mercaptoethanol. The system described has the advantages of being highly efficient and reproducible and utilises small amounts of cells, medium and antigen. It provides a simple, economic and reliable approach for the systematic study of the immune response in vitro."}
{"id": "PMID:1082002", "title": "Influenza virus infection in nude mice.", "content": "The role of T-cell function in influenza virus infection was studied by aerosol infection of nude mice with an influenza A virus (PR-8 strain). Nude mice died somewhat later than normal mice, and the antibody response of nude mice to the virus was minimal. Furthermore, nude mice did not eliminate the virus, which persisted for relatively long periods (two to three weeks).", "contents": "Influenza virus infection in nude mice. The role of T-cell function in influenza virus infection was studied by aerosol infection of nude mice with an influenza A virus (PR-8 strain). Nude mice died somewhat later than normal mice, and the antibody response of nude mice to the virus was minimal. Furthermore, nude mice did not eliminate the virus, which persisted for relatively long periods (two to three weeks)."}
{"id": "PMID:1082003", "title": "Electrophoretic heterogeneity of normal factor VIII/Von Willebrand protein, and abnormal electrophoretic mobility in patients with Von Willebrand's disease.", "content": "Crossed antigen-antibody electrophoresis was used to determine the electrophoretic properties of factor VIII/Von Willebrand (F. VIII/VW) protein in normal plasma using a specific rabbit antiserum against purified human factor VIII. The electrohoretic patterns in seven patients with severe Von Willebrand's disease in two different families were studied. In these patients the prolonged bleeding time was related to a functionally abnormal F. VIII/VW protein which was unable to induce platelet aggregation in presence of ristocetin. F. VIII/VW protein had an increased electrophoretic mobility in all the patients. The electrophoretic pattern was similar in different members of the same family but differed from one family to the other. Plasma samples collected after transfusion of normal cryoprecipitate to one member of the first family showed an increasing amount of F. VIII/VW protein, but even one hour after transfusion only one peak migration in an abnormal position was found. However, when mixed in vitro with normal plasma, the plasmas of the same patient showed two peaks, one in position of normal F. VIII/VW protein and one with increased electrophoretic mobility. These results suggest that normal F. VIII/VW protein transfused to recipients with Von Willebrand's disease synthesizing a functionally deficient factor undergoes a rapid alteration.", "contents": "Electrophoretic heterogeneity of normal factor VIII/Von Willebrand protein, and abnormal electrophoretic mobility in patients with Von Willebrand's disease. Crossed antigen-antibody electrophoresis was used to determine the electrophoretic properties of factor VIII/Von Willebrand (F. VIII/VW) protein in normal plasma using a specific rabbit antiserum against purified human factor VIII. The electrohoretic patterns in seven patients with severe Von Willebrand's disease in two different families were studied. In these patients the prolonged bleeding time was related to a functionally abnormal F. VIII/VW protein which was unable to induce platelet aggregation in presence of ristocetin. F. VIII/VW protein had an increased electrophoretic mobility in all the patients. The electrophoretic pattern was similar in different members of the same family but differed from one family to the other. Plasma samples collected after transfusion of normal cryoprecipitate to one member of the first family showed an increasing amount of F. VIII/VW protein, but even one hour after transfusion only one peak migration in an abnormal position was found. However, when mixed in vitro with normal plasma, the plasmas of the same patient showed two peaks, one in position of normal F. VIII/VW protein and one with increased electrophoretic mobility. These results suggest that normal F. VIII/VW protein transfused to recipients with Von Willebrand's disease synthesizing a functionally deficient factor undergoes a rapid alteration."}
{"id": "PMID:1082006", "title": "Stimulus-secretion coupling in an insect salivary gland: cell activation by elevated potassium concentrations.", "content": "1. Fluid secretion by isolated salivary glands was stimulated by elevating the external potassium concentration. 2. The stimulatory effect of potassium was dependent on external calcium and was potentiated by a subthreshold dose of 5-hydroxytryptamine (5-HT). 3. During the action of 120 mM potassium there was a large calciumdependent decrease in transepithelial resistance similar to that produced with 5-HT at normal potassium concentrations. 4. These results on Calliphora salivary glands are compared with other cases where cells are activated by high potassium. In most cases, the effect of high potassium is dependent upon calcium, suggesting that the latter plays a primary role in cell activation.", "contents": "Stimulus-secretion coupling in an insect salivary gland: cell activation by elevated potassium concentrations. 1. Fluid secretion by isolated salivary glands was stimulated by elevating the external potassium concentration. 2. The stimulatory effect of potassium was dependent on external calcium and was potentiated by a subthreshold dose of 5-hydroxytryptamine (5-HT). 3. During the action of 120 mM potassium there was a large calciumdependent decrease in transepithelial resistance similar to that produced with 5-HT at normal potassium concentrations. 4. These results on Calliphora salivary glands are compared with other cases where cells are activated by high potassium. In most cases, the effect of high potassium is dependent upon calcium, suggesting that the latter plays a primary role in cell activation."}
{"id": "PMID:1082007", "title": "Combining medical information with a business data system.", "content": "The School of Primary Medical Care at the University of Alabama in Huntsville is presently developing and implementing a computerized clinical data system that incorporates data from the Family Practice Center and the community hospital. The system can be used for the problem-oriented medical record, business and billing, the teaching program, community medicine research, and the evaluation of medical students and family practice residents. This paper discusses plans for the total system and the present degree of implementation.", "contents": "Combining medical information with a business data system. The School of Primary Medical Care at the University of Alabama in Huntsville is presently developing and implementing a computerized clinical data system that incorporates data from the Family Practice Center and the community hospital. The system can be used for the problem-oriented medical record, business and billing, the teaching program, community medicine research, and the evaluation of medical students and family practice residents. This paper discusses plans for the total system and the present degree of implementation."}
{"id": "PMID:1082009", "title": "The nutritional requirements of methicillin-dependent and -resistant strains of Pediococcus cerevisiae.", "content": "A new methicillin-dependent and -resistant substrain (called MRD) of Pediococcus cerevisiae was developed by serial passages followed by replica-plating. Other methicillin-resistant, but not -dependent, substrains were isolated after treatment of the same parent strain with a mutagen. A methicillin-independent partial revertant, still resistant to the drug, was isolated from the original methicillin-dependent and -resistant substrain (CRD) developed several years ago. The requirements of some of these strains for acetate, vitamins and amino acids were compared. All except the parent methicillin-sensitive strain required pantothenate for growth, but no other consistent differences were found. The parent, but not strain CRD, grew without lysine added to the medium, though 19 other amino acids were needed by each strain. Both of these strains fermented glucose to lactate (mainly the L-isomer) in the absence or presence of methicillin.", "contents": "The nutritional requirements of methicillin-dependent and -resistant strains of Pediococcus cerevisiae. A new methicillin-dependent and -resistant substrain (called MRD) of Pediococcus cerevisiae was developed by serial passages followed by replica-plating. Other methicillin-resistant, but not -dependent, substrains were isolated after treatment of the same parent strain with a mutagen. A methicillin-independent partial revertant, still resistant to the drug, was isolated from the original methicillin-dependent and -resistant substrain (CRD) developed several years ago. The requirements of some of these strains for acetate, vitamins and amino acids were compared. All except the parent methicillin-sensitive strain required pantothenate for growth, but no other consistent differences were found. The parent, but not strain CRD, grew without lysine added to the medium, though 19 other amino acids were needed by each strain. Both of these strains fermented glucose to lactate (mainly the L-isomer) in the absence or presence of methicillin."}
{"id": "PMID:1082011", "title": "Diagnosis and treatment of abscess of the central ganglia.", "content": "The authors present four cases of abscess of the thalamus and basal ganglia and review an additional 10 cases from the literature. These abscesses appear to be metastatic and are usually associated with a cardiac septal defect or a preceding intrathoracic infection. Fever, elevated white blood count, and/or meningismus, in combination with angiography demonstrating an avascular mass, help distinguish abscess from tumor in this location. The authors advocate aspiration rather than excision.", "contents": "Diagnosis and treatment of abscess of the central ganglia. The authors present four cases of abscess of the thalamus and basal ganglia and review an additional 10 cases from the literature. These abscesses appear to be metastatic and are usually associated with a cardiac septal defect or a preceding intrathoracic infection. Fever, elevated white blood count, and/or meningismus, in combination with angiography demonstrating an avascular mass, help distinguish abscess from tumor in this location. The authors advocate aspiration rather than excision."}
{"id": "PMID:1082012", "title": "Fluoroscopy of foramen ovale as an aid to thermocoagulation of the Gasserian ganglion; technical note.", "content": "The authors described a fluoroscopic method of guiding percutaneous needle penetration of the foramen ovale. The advantages are simplicity, speed, accuracy, and comfortable patient positioning. Radiation exposure is minimized.", "contents": "Fluoroscopy of foramen ovale as an aid to thermocoagulation of the Gasserian ganglion; technical note. The authors described a fluoroscopic method of guiding percutaneous needle penetration of the foramen ovale. The advantages are simplicity, speed, accuracy, and comfortable patient positioning. Radiation exposure is minimized."}
{"id": "PMID:1082014", "title": "Effects of luminance and stimulus distance on accommodation and visual resolution.", "content": "Measurements of accommodation and visual resolution were obtained at a number of luminance levels and stimulus distances. With reductions in luminance the eye approached a \"fixed-focus\" condition of accommodation for intermediate distances, resulting in successively larger errors in accommodation for both near and far stimuli. The visual resolution values were initially affected by both the luminance and distance of the stimulus. Subsequent measurements of resolution, following the correction of accommodative errors, were found to be independent of the stimulus distance. The findings are discussed with regard to the problems of \"night myopia\" and variations in visual resolution with stimulus distance.", "contents": "Effects of luminance and stimulus distance on accommodation and visual resolution. Measurements of accommodation and visual resolution were obtained at a number of luminance levels and stimulus distances. With reductions in luminance the eye approached a \"fixed-focus\" condition of accommodation for intermediate distances, resulting in successively larger errors in accommodation for both near and far stimuli. The visual resolution values were initially affected by both the luminance and distance of the stimulus. Subsequent measurements of resolution, following the correction of accommodative errors, were found to be independent of the stimulus distance. The findings are discussed with regard to the problems of \"night myopia\" and variations in visual resolution with stimulus distance."}
{"id": "PMID:1082015", "title": "Blood loss and hypotensive anesthesia in oral-facial corrective surgery.", "content": "To evaluate accurately changes in blood volume during oral-facial corrective surgery, double-tagged radioisotope blood volume studies were performed preoperatively and postoperatively. Unexpectedly large RCV deficits that were not consistent with EBL were observed in studies conducted at two separate facilities. Hypotensive general anesthesia was used for the intended purpose of reducing blood loss in oral-facial corrective surgery. Various physiological parameters were measured preoperatively, intraoperatively, and postoperatively to evaluate patient response to this anesthetic method. The MAP was reduced from 89.94 +/- 2.13 to 72.79 +/- 1.63 mm Hg under the influence of NaN infusion supplemented with halothane. No dose-response relationship existed between NaN and the intraoperative MAP. A 44% reduction in the measured red cell volume was observed in comparison to a previously studied group of patients who underwent similar surgical procedures under normotensive anesthesia. It is concluded from an extensive review of the literature that the most vulnerable period for serious complications is in the immediate postoperative period. No significant complications or adverse effects were observed in this study. It cannot be emphasized too strongly that the safety of this hypotensive technique lies in constant and careful monitoring of the patient and meticulous attention to all details of anesthetic management.", "contents": "Blood loss and hypotensive anesthesia in oral-facial corrective surgery. To evaluate accurately changes in blood volume during oral-facial corrective surgery, double-tagged radioisotope blood volume studies were performed preoperatively and postoperatively. Unexpectedly large RCV deficits that were not consistent with EBL were observed in studies conducted at two separate facilities. Hypotensive general anesthesia was used for the intended purpose of reducing blood loss in oral-facial corrective surgery. Various physiological parameters were measured preoperatively, intraoperatively, and postoperatively to evaluate patient response to this anesthetic method. The MAP was reduced from 89.94 +/- 2.13 to 72.79 +/- 1.63 mm Hg under the influence of NaN infusion supplemented with halothane. No dose-response relationship existed between NaN and the intraoperative MAP. A 44% reduction in the measured red cell volume was observed in comparison to a previously studied group of patients who underwent similar surgical procedures under normotensive anesthesia. It is concluded from an extensive review of the literature that the most vulnerable period for serious complications is in the immediate postoperative period. No significant complications or adverse effects were observed in this study. It cannot be emphasized too strongly that the safety of this hypotensive technique lies in constant and careful monitoring of the patient and meticulous attention to all details of anesthetic management."}
{"id": "PMID:1082017", "title": "Liver disease associated with alpha1-antitrypsin deficiency in childhood.", "content": "Liver disease in children with alpha1-antitrypsin deficiency and protease inhibitor type ZZ does not necessarily carry a bad prognosis. Fourteen of our 18 patients presented with the neonatal hepatitis syndrome and four had hepatomegaly without jaundice. Although four patients have died of cirrhosis and its complications, and three have severe liver disease, most of the 11 others, of whom four are over 13 years of age, have relatively little clinical, biochemical, or histologic evidence of liver disease. Persistent elevation of SGOT during the third year of life and renal or pulmonary problems were associated with a poor prognosis. Liver biopsy early in the course of the disease was not helpful prognostically but was useful in assessment of the severity of liver disease and demonstration of alpha1AT storage, alpha1AT deficiency was found in 29% of our patients who presented with the neonatal hepatitis syndrome. One of seven apparently healthy Pi type ZZ sibs of our patients had significant liver disease which had not been suspected previously.", "contents": "Liver disease associated with alpha1-antitrypsin deficiency in childhood. Liver disease in children with alpha1-antitrypsin deficiency and protease inhibitor type ZZ does not necessarily carry a bad prognosis. Fourteen of our 18 patients presented with the neonatal hepatitis syndrome and four had hepatomegaly without jaundice. Although four patients have died of cirrhosis and its complications, and three have severe liver disease, most of the 11 others, of whom four are over 13 years of age, have relatively little clinical, biochemical, or histologic evidence of liver disease. Persistent elevation of SGOT during the third year of life and renal or pulmonary problems were associated with a poor prognosis. Liver biopsy early in the course of the disease was not helpful prognostically but was useful in assessment of the severity of liver disease and demonstration of alpha1AT storage, alpha1AT deficiency was found in 29% of our patients who presented with the neonatal hepatitis syndrome. One of seven apparently healthy Pi type ZZ sibs of our patients had significant liver disease which had not been suspected previously."}
{"id": "PMID:1082018", "title": "An outbreak of Hemophilus influenzae type b meningitis in an enclosed hospital population.", "content": "Five cases of HITB maningitis occurred within six months in an enclosed population of 28 to 32 chronically ill children. Studies of nasopharyngeal carriage and serum HITB anticapsular antibodies were started after the third case occurred. Two patients had low (less than 0.04 and 0.05mug/ml) antibodies and were not carriers when studied prior to onset of their disease. The carriage rate was approximately 20% among the children. Carriage was usually prolonged, and acquisition was not prevented by high antibody levels. Attempts to arrest this outbreak with type b polysaccharide immunization and ampicillin therapy are discussed in the context of HITB meningitis as a contagious disease.", "contents": "An outbreak of Hemophilus influenzae type b meningitis in an enclosed hospital population. Five cases of HITB maningitis occurred within six months in an enclosed population of 28 to 32 chronically ill children. Studies of nasopharyngeal carriage and serum HITB anticapsular antibodies were started after the third case occurred. Two patients had low (less than 0.04 and 0.05mug/ml) antibodies and were not carriers when studied prior to onset of their disease. The carriage rate was approximately 20% among the children. Carriage was usually prolonged, and acquisition was not prevented by high antibody levels. Attempts to arrest this outbreak with type b polysaccharide immunization and ampicillin therapy are discussed in the context of HITB meningitis as a contagious disease."}
{"id": "PMID:1082023", "title": "Mechanical properties of frog skeletal muscles in iodoacetic acid rigor.", "content": "1. Methods have been developed for describing the length: tension characteristics of frog skeletal muscles which go into rigor at 4 degrees C following iodoacetic acid poisoning either in the presence of Ca2+ (Ca-rigor) or its absence (Ca-free-rigor). 2. Such rigor muscles showed less resistance to slow stretch (slow rigor resistance) that to fast stretch (fast rigor resistance). The slow and fast rigor resistances of Ca-free-rigor muscles were much lower than those of Ca-rigor muscles. 3. The slow rigor resistance of Ca-rigor muscles was proportional to the amount of overlap between the contractile filaments present when the muscles were put into rigor. 4. Withdrawing Ca2+ from Ca-rigor muscles (induced-Ca-free rigor) reduced their slow and fast rigor resistances. Readdition of Ca2+ (but not Mg2+, Mn2+ or Sr2+) reversed the effect. 5. The slow and fast rigor resistances of Ca-rigor muscles (but not of Ca-free-rigor muscles) decreased with time. 6. The sarcomere structure of Ca-rigor and induced-Ca-free rigor muscles stretched by 0.2lo was destroyed in proportion to the amount of stretch, but the lengths of the remaining intact sarcomeres were essentially unchanged. This suggests that there had been a successive yielding of the weakeast sarcomeres. 7. The difference between the slow and fast rigor resistance and the effect of calcium on these resistances are discussed in relation to possible variations in the strength of crossbridges between the thick and thin filaments.", "contents": "Mechanical properties of frog skeletal muscles in iodoacetic acid rigor. 1. Methods have been developed for describing the length: tension characteristics of frog skeletal muscles which go into rigor at 4 degrees C following iodoacetic acid poisoning either in the presence of Ca2+ (Ca-rigor) or its absence (Ca-free-rigor). 2. Such rigor muscles showed less resistance to slow stretch (slow rigor resistance) that to fast stretch (fast rigor resistance). The slow and fast rigor resistances of Ca-free-rigor muscles were much lower than those of Ca-rigor muscles. 3. The slow rigor resistance of Ca-rigor muscles was proportional to the amount of overlap between the contractile filaments present when the muscles were put into rigor. 4. Withdrawing Ca2+ from Ca-rigor muscles (induced-Ca-free rigor) reduced their slow and fast rigor resistances. Readdition of Ca2+ (but not Mg2+, Mn2+ or Sr2+) reversed the effect. 5. The slow and fast rigor resistances of Ca-rigor muscles (but not of Ca-free-rigor muscles) decreased with time. 6. The sarcomere structure of Ca-rigor and induced-Ca-free rigor muscles stretched by 0.2lo was destroyed in proportion to the amount of stretch, but the lengths of the remaining intact sarcomeres were essentially unchanged. This suggests that there had been a successive yielding of the weakeast sarcomeres. 7. The difference between the slow and fast rigor resistance and the effect of calcium on these resistances are discussed in relation to possible variations in the strength of crossbridges between the thick and thin filaments."}
{"id": "PMID:1082024", "title": "Discrete and discontinuous action of brown widow spider venom on the presynaptic nerve terminals of frog muscle.", "content": "1. A study was made of the effects of the venom of the brown widow spider (Latrodectus geometricus) on end-plates of the frog sartorius muscle. 2. The increase in the frequency of the minature end-plate potentials  (m.e.p.p.s), elicited by the venom in normal-\"Ca2+\" Ringer solution, occurs in discrete volleys having a sharp onset and end. The frequency of the m.e.p.p.s is high (up to 300 sec-1) and relatively constant during the volley. 3. The volleys recur at intervals during a period from 5 to 10 min after addition of the venom until the onset of electrical silence, up to 4 hr later. The activity occurs in groups containing volleys of loing and short duration. 4. Simultaneous intracellular and extracellular recording from single end-plates indicates that the volleys originate at highly localized areas of the nerve terminals. The high-frequency release of m.e.p.p.s in hypertonic sol solutions, which was studied for comparison purposes, occurs randomly over the entire end-plate. Volleys originating simultaneously at different sites are often superimposed in the intracellular recordings. 5. In high-\"Ca2+\" Ringer solution, the initial frequency of the m.e.p.p.s in a volley is comparatively higher. However, the frequency drops to one half its value in a few seconds. The volley then terminates or else the frequency of m.e.p.p.s remains high for some time and the volley has no sharp end. Activity occurs in groups containing both long and short volleys. Many more short (less than 5 sec) and long (greater than 30 sec) volleys occur in high-\"Ca2+\" solution than in normal-\"Ca2+\" solutions. 6. In low-\"Ca2+\", high-\"Mg2+\" Ringer solution, the volleys of m.e.p.p.s are fewer in number and much longer in duration. Intra- and extra-cellular recording of uninterruped activity during long periods suggests that in this solution the m.e.p.p.s originate diffusely rather than at discrete areas of the nerve terminals. 7. Implications of the above data on possible modes of action of the venom are discussed.", "contents": "Discrete and discontinuous action of brown widow spider venom on the presynaptic nerve terminals of frog muscle. 1. A study was made of the effects of the venom of the brown widow spider (Latrodectus geometricus) on end-plates of the frog sartorius muscle. 2. The increase in the frequency of the minature end-plate potentials  (m.e.p.p.s), elicited by the venom in normal-\"Ca2+\" Ringer solution, occurs in discrete volleys having a sharp onset and end. The frequency of the m.e.p.p.s is high (up to 300 sec-1) and relatively constant during the volley. 3. The volleys recur at intervals during a period from 5 to 10 min after addition of the venom until the onset of electrical silence, up to 4 hr later. The activity occurs in groups containing volleys of loing and short duration. 4. Simultaneous intracellular and extracellular recording from single end-plates indicates that the volleys originate at highly localized areas of the nerve terminals. The high-frequency release of m.e.p.p.s in hypertonic sol solutions, which was studied for comparison purposes, occurs randomly over the entire end-plate. Volleys originating simultaneously at different sites are often superimposed in the intracellular recordings. 5. In high-\"Ca2+\" Ringer solution, the initial frequency of the m.e.p.p.s in a volley is comparatively higher. However, the frequency drops to one half its value in a few seconds. The volley then terminates or else the frequency of m.e.p.p.s remains high for some time and the volley has no sharp end. Activity occurs in groups containing both long and short volleys. Many more short (less than 5 sec) and long (greater than 30 sec) volleys occur in high-\"Ca2+\" solution than in normal-\"Ca2+\" solutions. 6. In low-\"Ca2+\", high-\"Mg2+\" Ringer solution, the volleys of m.e.p.p.s are fewer in number and much longer in duration. Intra- and extra-cellular recording of uninterruped activity during long periods suggests that in this solution the m.e.p.p.s originate diffusely rather than at discrete areas of the nerve terminals. 7. Implications of the above data on possible modes of action of the venom are discussed."}
{"id": "PMID:1082025", "title": "Two mechanisms for the meperidine block of action potential production in frog's skeletal muscle; non-specific and opiate drug receptor mediated blockade.", "content": "The effects of meperidine and naloxone, and their interaction effects on action potential production in frog's sartorius muscle fibres, were studied with intracellular micro-electrode techniques. 1. Meperidine, a narcotic analgesic drug, depressed the rate of rise, the rate of fall and the amplitude of the action potentials. 2. At a meperidine concentration of 0-35 mM, the depression in the action potential maximum rate of rise followed a diphasic time course. At first there was a rapid reduction in the maximum rate of rise which was levelling off at about 60% of control 60-90 min after drug application. This was followed by the second phase during which there was an initial rapid decrease in the maximum rate of rise and all surface fibres were inexcitable by 180 min. 3. The addition of naloxone, a narcotic antagonist, in low concentrations (3 X 10(-5) to 3 X 10(-4) mM) at 70-90 min blocked the second phase of the meperidine-induced depression. 4. With lower concentrations of meperidine (0-18 and 0-07 mM) the depression usually developed more slowly (up to 6 hr with the latter dose) and the addition of low naloxone concentrations partially antagonized the effects of meperidine. However, under no conditions was it possible to completely antagonize the effects of meperidine by the addition of naloxone. 5. A linear relation was found between action potential amplitude and the action potential maximum rate of fall. 6. Meperidine caused a shift in the relation of rate of fall against amplitude to higher action potential amplitudes, indicating that the drug inhibited the increase in potassium conductivity (gK) associated with the falling phase of the action potential. 7. When low naloxone concentrations antagonized the effects of meperidine on the rate of rise and restored action potential amplitudes to control levels, the effect of meperidine on the maximum rate of fall was not antagonized. 8. Larger naloxone concentrations (1-5 X 10(-2) mM or more) depressed the action potential rate of rise but did not alter the relation between action potential amplitude and the maximum rate of fall. 9. It is proposed that meperidine blocks action potential production by two mechanisms: (i) a non-specific mechanism in which the increases in both gNa and gK ar depressed and (ii) an opiate drug receptor mediated mechanism causing a specific depression of gNa. 10. The impression gained from the results is that there are opiate drug receptors located on the inner surface of the muscle membrane associated with the 'sodium channels' and that drug activation of these receptors by either meperidine or high naloxone concentrations interferes with the opening of the 'sodium channels' normally produced by membrane depolarization.", "contents": "Two mechanisms for the meperidine block of action potential production in frog's skeletal muscle; non-specific and opiate drug receptor mediated blockade. The effects of meperidine and naloxone, and their interaction effects on action potential production in frog's sartorius muscle fibres, were studied with intracellular micro-electrode techniques. 1. Meperidine, a narcotic analgesic drug, depressed the rate of rise, the rate of fall and the amplitude of the action potentials. 2. At a meperidine concentration of 0-35 mM, the depression in the action potential maximum rate of rise followed a diphasic time course. At first there was a rapid reduction in the maximum rate of rise which was levelling off at about 60% of control 60-90 min after drug application. This was followed by the second phase during which there was an initial rapid decrease in the maximum rate of rise and all surface fibres were inexcitable by 180 min. 3. The addition of naloxone, a narcotic antagonist, in low concentrations (3 X 10(-5) to 3 X 10(-4) mM) at 70-90 min blocked the second phase of the meperidine-induced depression. 4. With lower concentrations of meperidine (0-18 and 0-07 mM) the depression usually developed more slowly (up to 6 hr with the latter dose) and the addition of low naloxone concentrations partially antagonized the effects of meperidine. However, under no conditions was it possible to completely antagonize the effects of meperidine by the addition of naloxone. 5. A linear relation was found between action potential amplitude and the action potential maximum rate of fall. 6. Meperidine caused a shift in the relation of rate of fall against amplitude to higher action potential amplitudes, indicating that the drug inhibited the increase in potassium conductivity (gK) associated with the falling phase of the action potential. 7. When low naloxone concentrations antagonized the effects of meperidine on the rate of rise and restored action potential amplitudes to control levels, the effect of meperidine on the maximum rate of fall was not antagonized. 8. Larger naloxone concentrations (1-5 X 10(-2) mM or more) depressed the action potential rate of rise but did not alter the relation between action potential amplitude and the maximum rate of fall. 9. It is proposed that meperidine blocks action potential production by two mechanisms: (i) a non-specific mechanism in which the increases in both gNa and gK ar depressed and (ii) an opiate drug receptor mediated mechanism causing a specific depression of gNa. 10. The impression gained from the results is that there are opiate drug receptors located on the inner surface of the muscle membrane associated with the 'sodium channels' and that drug activation of these receptors by either meperidine or high naloxone concentrations interferes with the opening of the 'sodium channels' normally produced by membrane depolarization."}
{"id": "PMID:1082026", "title": "A study of hypothalmic neurosecretory cells of bullfrogs in vitro.", "content": "Neurosecretory cells of preoptic nuclei of bullfrogs were studied in isolated hypothalamo-hypophysial preparations under constant perfusion with oxygenated Ringer solution at 15-17 degress C. Antidromic potentials were recorded following stimuli applied to the posterior lobe of the pituitary or the stalk. 2. Intracellularly and extracellularly recorded potentials resembled those obtained in vivo from neurosecretory cells of the mammalian hypothalamus. They were unique in that the antidromic potential had a long duration (10-20 msec) and a distinct notch on the rising phase (between A and B spikes). The conduction velocity of the stalk fibres in vitro at this temperature was 0-1--0-2 m/sec. 3. When two successive stimuli were given to the posterior lobe or to the stalk separated by intervals of between 30 and 65 msec, the test (second) response showed a longer delay of the B spike. This delay between the A and B components was as long as 10 msec. Further shortening of stimulus intervals produced block of B spikes in the test response. A complete separation of A and B spikes occur spontaneously in a few instances. 4. Evidence indicated that inhibitory recurrent axon collaterals play a role in the control of bullfrog neurosecretory cells. Antidromic potentials were inhibited by a 'conditioning' stimulus for as long as 300-400 msec, even when the stimulus did not evoke an antidromic potential. 5. It was found that in addition to the inhibitory interaction there is a facilitatory recurrent axon collateral system which operates within the nuclei. The evidence for this is: (1) stimulation of the posterior lobe, with single subthreshold pulses evoked an action potential if preceded by another stimulus of subthreshold or just threshold intensity. The durations of such facilitatory effects were found to be 20--400 msec; (2) a single pulse given to the posterior lobe did occasionally evoke two spikes from neurosecretory cells; the second spike which occurred 15-30 msec after the first had the characteristics of a trans-synaptically produced potential; (3) gradual changes in the intensity of stimuli applied to the neural lobe produced a sudden shift in latencies ranging between 15 and 30 msec. The potentials having long latency also showed characteristics of those transsynaptically excited. In addition, an increase in excitability of neurosecretory cells by antidromic stimulation was confirmed by using orthodromically induced action potentials in in vivo studies. Possible functional significance of inhibitory and excitatory recurrent collateral system in neurosecretory cells was discussed. 6. Two to threefold increase in NaCl concentration of a perfusate slightly increased the latency and refractory period of antidromic potential but not the shape of the potential. Norepinephrine added to a perfusate (1 mug/ml). augmented the separation of A and B spikes of the antidromic potential. Acetylcholine at a concentration of 1 mug/ml. did not have an appreciable effect on the antidromic potential.", "contents": "A study of hypothalmic neurosecretory cells of bullfrogs in vitro. Neurosecretory cells of preoptic nuclei of bullfrogs were studied in isolated hypothalamo-hypophysial preparations under constant perfusion with oxygenated Ringer solution at 15-17 degress C. Antidromic potentials were recorded following stimuli applied to the posterior lobe of the pituitary or the stalk. 2. Intracellularly and extracellularly recorded potentials resembled those obtained in vivo from neurosecretory cells of the mammalian hypothalamus. They were unique in that the antidromic potential had a long duration (10-20 msec) and a distinct notch on the rising phase (between A and B spikes). The conduction velocity of the stalk fibres in vitro at this temperature was 0-1--0-2 m/sec. 3. When two successive stimuli were given to the posterior lobe or to the stalk separated by intervals of between 30 and 65 msec, the test (second) response showed a longer delay of the B spike. This delay between the A and B components was as long as 10 msec. Further shortening of stimulus intervals produced block of B spikes in the test response. A complete separation of A and B spikes occur spontaneously in a few instances. 4. Evidence indicated that inhibitory recurrent axon collaterals play a role in the control of bullfrog neurosecretory cells. Antidromic potentials were inhibited by a 'conditioning' stimulus for as long as 300-400 msec, even when the stimulus did not evoke an antidromic potential. 5. It was found that in addition to the inhibitory interaction there is a facilitatory recurrent axon collateral system which operates within the nuclei. The evidence for this is: (1) stimulation of the posterior lobe, with single subthreshold pulses evoked an action potential if preceded by another stimulus of subthreshold or just threshold intensity. The durations of such facilitatory effects were found to be 20--400 msec; (2) a single pulse given to the posterior lobe did occasionally evoke two spikes from neurosecretory cells; the second spike which occurred 15-30 msec after the first had the characteristics of a trans-synaptically produced potential; (3) gradual changes in the intensity of stimuli applied to the neural lobe produced a sudden shift in latencies ranging between 15 and 30 msec. The potentials having long latency also showed characteristics of those transsynaptically excited. In addition, an increase in excitability of neurosecretory cells by antidromic stimulation was confirmed by using orthodromically induced action potentials in in vivo studies. Possible functional significance of inhibitory and excitatory recurrent collateral system in neurosecretory cells was discussed. 6. Two to threefold increase in NaCl concentration of a perfusate slightly increased the latency and refractory period of antidromic potential but not the shape of the potential. Norepinephrine added to a perfusate (1 mug/ml). augmented the separation of A and B spikes of the antidromic potential. Acetylcholine at a concentration of 1 mug/ml. did not have an appreciable effect on the antidromic potential."}
{"id": "PMID:1082027", "title": "Calcium content and exchange in frog skeletal muscle.", "content": "Calcium content and exchange in frog ELD IV muscle were examined employing the efflux technique. 2. Muscle calcium was found to exchange with four time constants, 21-5 sec 2-7, 32 and 1244 min. 3. All calcium was found to be exchangeable with more than half the total amount residing in an extracellular compartment. 4. Results obtained from ELD IV muscles and single fibres were identical. 5. Muscle calcium content was found to remain constant up to 20 hr in vitro. 6. Extra exchange of calcium occurs upon contraction. This extra exchange appears to occur in the most slowly exchanging component. 7. The data are discussed in relation to morphological and autoradiographic findings and a model of calcium exchange in skeletal muscle is presented.", "contents": "Calcium content and exchange in frog skeletal muscle. Calcium content and exchange in frog ELD IV muscle were examined employing the efflux technique. 2. Muscle calcium was found to exchange with four time constants, 21-5 sec 2-7, 32 and 1244 min. 3. All calcium was found to be exchangeable with more than half the total amount residing in an extracellular compartment. 4. Results obtained from ELD IV muscles and single fibres were identical. 5. Muscle calcium content was found to remain constant up to 20 hr in vitro. 6. Extra exchange of calcium occurs upon contraction. This extra exchange appears to occur in the most slowly exchanging component. 7. The data are discussed in relation to morphological and autoradiographic findings and a model of calcium exchange in skeletal muscle is presented."}
{"id": "PMID:1082029", "title": "Lymphocyte subpopulations in normals and patients with rheumatoid arthritis and ankylosing spondylitis.", "content": "Seventy-four normal persons, 48 patients with rheumatoid arthritis, and 25 patients with ankylosing spondylitis, were examined in respect to lymphocyte subpopulations in the peripheral blood. The simultaneous enumeration of T lymphocytes (E rosettes), B lymphocytes (surface immunoglobulin), as well as null cells and double marker cells was performed. No significant differences could be observed in the three examined groups in respect to the absolute or the relative lymphocyte counts, suggesting that possible immunological impairment assumed in rheumatoid arthritis and ankylosing spondylitis, is due not to reduced cell numbers, but to a possible functional disorder.", "contents": "Lymphocyte subpopulations in normals and patients with rheumatoid arthritis and ankylosing spondylitis. Seventy-four normal persons, 48 patients with rheumatoid arthritis, and 25 patients with ankylosing spondylitis, were examined in respect to lymphocyte subpopulations in the peripheral blood. The simultaneous enumeration of T lymphocytes (E rosettes), B lymphocytes (surface immunoglobulin), as well as null cells and double marker cells was performed. No significant differences could be observed in the three examined groups in respect to the absolute or the relative lymphocyte counts, suggesting that possible immunological impairment assumed in rheumatoid arthritis and ankylosing spondylitis, is due not to reduced cell numbers, but to a possible functional disorder."}
{"id": "PMID:1082030", "title": "Cellular and humoral immune parameters of synovial fluid and blood in rheumatoid arthritis.", "content": "Thymus derived (T) and bone marrow derived (B) cells from joint fluid and peripheral blood of 15 patients with rheumatoid arthritis and 10 controls with other inflammatory joint diseases were studied. High absolute numbers and high percentages of T cells in the synovial fluid, T plus B cell sums exceeding 100 per cent in synovial fluid or blood, and fewer complement receptors compared to surface Ig bearing cells in joint fluid or blood was found only in rheumatoid arthritis. T and B cell numbers did not correlate with disease activity. No differences in T and B cells were found between patients positive or negative for rheumatoid factor. (J Rheumatol 2: 363-372, 1975).", "contents": "Cellular and humoral immune parameters of synovial fluid and blood in rheumatoid arthritis. Thymus derived (T) and bone marrow derived (B) cells from joint fluid and peripheral blood of 15 patients with rheumatoid arthritis and 10 controls with other inflammatory joint diseases were studied. High absolute numbers and high percentages of T cells in the synovial fluid, T plus B cell sums exceeding 100 per cent in synovial fluid or blood, and fewer complement receptors compared to surface Ig bearing cells in joint fluid or blood was found only in rheumatoid arthritis. T and B cell numbers did not correlate with disease activity. No differences in T and B cells were found between patients positive or negative for rheumatoid factor. (J Rheumatol 2: 363-372, 1975)."}
{"id": "PMID:1082031", "title": "Acute mitral regurgitation from ruptured chordae tendineae in systemic lupus erythematosus.", "content": "A patient with systemic lupus erythematosus developed acute mitral regurgitation due to ruptured chordae tendineae, requiring mitral valve replacement. Typical changes of Libman-Sacks endocarditis were observed in the excised mitral valve. Immunofluorescent studies revealed antinuclear antibody and deposits of immunoglobulins and complement within small vessels.", "contents": "Acute mitral regurgitation from ruptured chordae tendineae in systemic lupus erythematosus. A patient with systemic lupus erythematosus developed acute mitral regurgitation due to ruptured chordae tendineae, requiring mitral valve replacement. Typical changes of Libman-Sacks endocarditis were observed in the excised mitral valve. Immunofluorescent studies revealed antinuclear antibody and deposits of immunoglobulins and complement within small vessels."}
{"id": "PMID:1082034", "title": "Development of two manifestations of T-lymphocyte reactivity during tumor growth: altered kinetics associated with elevated growth rates.", "content": "Tumor-specific, cell-mediated immune responses (CMI) in mice were measured during the growth of transplantable tumors with the use of lymphocyte-mediated cytotoxicity and the recently developed leukocyte adherence inhibition (LAI) microtest. When both tests were used in parallel to assess CMI, no consistent correlation was found, which suggested that each test was a measure of the activity of a functionally different T-lymphocyte subpopulation. The results of blocking factor (BF) assays with both tests agreed in 78% of the instances; the LAI microtest was much more sensitive for BF determination than lymphocyte cytotoxicity, since blocking serum concentrations as low as 0.25% could demonstrate BF activity in the microstest. In other experiments, the kinetics of development of tumor-specific CMI was followed in normal mice and those under toxic stress; the stressed animals showed depressed humoral and cell-mediated immune responses and enhanced tumor growth rates after the inoculation of tumor cells. In normal mice, a cytotoxic response always appeared in the spleens and regional lymph nodes 3-5 days before an LAI response. Enhanced tumor growth rates in the animals under toxic stress were associated with a reversal in the order in which these two manifestations of T-lymphocyte responsiveness developed; LAI responses always developed before, and often in the complete absence of, cytotoxicity.", "contents": "Development of two manifestations of T-lymphocyte reactivity during tumor growth: altered kinetics associated with elevated growth rates. Tumor-specific, cell-mediated immune responses (CMI) in mice were measured during the growth of transplantable tumors with the use of lymphocyte-mediated cytotoxicity and the recently developed leukocyte adherence inhibition (LAI) microtest. When both tests were used in parallel to assess CMI, no consistent correlation was found, which suggested that each test was a measure of the activity of a functionally different T-lymphocyte subpopulation. The results of blocking factor (BF) assays with both tests agreed in 78% of the instances; the LAI microtest was much more sensitive for BF determination than lymphocyte cytotoxicity, since blocking serum concentrations as low as 0.25% could demonstrate BF activity in the microstest. In other experiments, the kinetics of development of tumor-specific CMI was followed in normal mice and those under toxic stress; the stressed animals showed depressed humoral and cell-mediated immune responses and enhanced tumor growth rates after the inoculation of tumor cells. In normal mice, a cytotoxic response always appeared in the spleens and regional lymph nodes 3-5 days before an LAI response. Enhanced tumor growth rates in the animals under toxic stress were associated with a reversal in the order in which these two manifestations of T-lymphocyte responsiveness developed; LAI responses always developed before, and often in the complete absence of, cytotoxicity."}
{"id": "PMID:1082037", "title": "Neonatal meningitis and mastoiditis caused by Hemophilus influenzae.", "content": "A newborn infant developed Hemophilus influenzae meningitis associated with acute coalescent mastoiditis and a cutaneous abscess in the mastoid region. Mastoidectomy was followed by prompt recovery from the meningitis, which had failed to clear previously despite antibiotic therapy. Mastoiditis may exist as an infective focus in neonatal meningitis more frequently than has been appreciated. Mastoid roentgenograms are usually the only clue to diagnosis of this infection and should be obtained in patients with neonatal meningitis responding poorly to antibiotic therapy.", "contents": "Neonatal meningitis and mastoiditis caused by Hemophilus influenzae. A newborn infant developed Hemophilus influenzae meningitis associated with acute coalescent mastoiditis and a cutaneous abscess in the mastoid region. Mastoidectomy was followed by prompt recovery from the meningitis, which had failed to clear previously despite antibiotic therapy. Mastoiditis may exist as an infective focus in neonatal meningitis more frequently than has been appreciated. Mastoid roentgenograms are usually the only clue to diagnosis of this infection and should be obtained in patients with neonatal meningitis responding poorly to antibiotic therapy."}
{"id": "PMID:1082038", "title": "\"Normal-pressure\" hydrocephalus. Relationship of clinical and radiographic findings to improvement following shunt surgery.", "content": "Analysis of 25 patients who fulfilled clinical and radiographic criteria for the diagnosis of \"normal pressure\" hydrocephalus (NPH) demonstrated (1) a significant relationship between presence of motor signs with good outcome and absence of motor signs with poor outcome following ventricular shunting, (2) symptoms and signs of parkinsonism in 40% of patients in whom the diagnosis of NPH was made, and (3) no reliable relationship between radiographic measurements or cisternogram appearance and outcome following shunting. The clinical picture is the most important factor in selection of NPH patients for surgery.", "contents": "\"Normal-pressure\" hydrocephalus. Relationship of clinical and radiographic findings to improvement following shunt surgery. Analysis of 25 patients who fulfilled clinical and radiographic criteria for the diagnosis of \"normal pressure\" hydrocephalus (NPH) demonstrated (1) a significant relationship between presence of motor signs with good outcome and absence of motor signs with poor outcome following ventricular shunting, (2) symptoms and signs of parkinsonism in 40% of patients in whom the diagnosis of NPH was made, and (3) no reliable relationship between radiographic measurements or cisternogram appearance and outcome following shunting. The clinical picture is the most important factor in selection of NPH patients for surgery."}
{"id": "PMID:1082041", "title": "Finding recent tuberculous infection in New Orleans. Results of tuberculin skin tests on New Orleans children from the inner city and Contact Investigation Program.", "content": "We tested infants and preschool-age children from the New Orleans Health Department programs for tuberculosis contact investigation and for tuberculin skin testing. About 7.8% (34 of 438) of the contacts and about 0.78% (107 of 13,797) of those given skin tests were tuberculosis-infected. Both programs, we concluded, detected enough recent tuberculous infection to be worthwhile as screening procedures.", "contents": "Finding recent tuberculous infection in New Orleans. Results of tuberculin skin tests on New Orleans children from the inner city and Contact Investigation Program. We tested infants and preschool-age children from the New Orleans Health Department programs for tuberculosis contact investigation and for tuberculin skin testing. About 7.8% (34 of 438) of the contacts and about 0.78% (107 of 13,797) of those given skin tests were tuberculosis-infected. Both programs, we concluded, detected enough recent tuberculous infection to be worthwhile as screening procedures."}
{"id": "PMID:1082044", "title": "Difference in calcium content of atrial and ventricular muscle.", "content": "The calcium content and inulin space of the atrial and ventricular muscle were determined in isolated perfused heart of the toad, bullfrog, guinea pig, rat, and cat. In all species studied, the total and \"cellular\" calcium content of the atrium were higher than those of the ventricle. A high calcium content of the atrial muscle was also observed in fresh unperfused hearts. The total calcium content of atrial and ventricular muscles increased when extracellular phosphate concentration was increased. When severe ventricular arrhythmia such as fibrillation occurred during perfusion, the total calcium content of the ventricle increased. The decay of tissue calcium content with calcium washout was examined in the toad, guinea pig, and rat heart. It was revealed that about 80 percent of total calcium existed as \"exchangeable\" calcium in both atrial and ventricular muscles. When the extracellular calcium concentration ([Ca]o) was altered in the toad, guinea pig, and rat heart preparation, the cellular calcium content of atrial muscle varied in proportion to [Ca]o, whereas that of ventricular muscle remained fairly constant at a higher [Ca]o value. The development of contractile tension in the atrial and ventricular muscles at various [Ca]o values corresponded well to these changes in the cellular calcium content except for the rat ventricle in which the contractile tension was almost proportional to [Ca]o. The relationship between the development of contractile tension and the level of cellular calcium content or [Ca]o was discussed.", "contents": "Difference in calcium content of atrial and ventricular muscle. The calcium content and inulin space of the atrial and ventricular muscle were determined in isolated perfused heart of the toad, bullfrog, guinea pig, rat, and cat. In all species studied, the total and \"cellular\" calcium content of the atrium were higher than those of the ventricle. A high calcium content of the atrial muscle was also observed in fresh unperfused hearts. The total calcium content of atrial and ventricular muscles increased when extracellular phosphate concentration was increased. When severe ventricular arrhythmia such as fibrillation occurred during perfusion, the total calcium content of the ventricle increased. The decay of tissue calcium content with calcium washout was examined in the toad, guinea pig, and rat heart. It was revealed that about 80 percent of total calcium existed as \"exchangeable\" calcium in both atrial and ventricular muscles. When the extracellular calcium concentration ([Ca]o) was altered in the toad, guinea pig, and rat heart preparation, the cellular calcium content of atrial muscle varied in proportion to [Ca]o, whereas that of ventricular muscle remained fairly constant at a higher [Ca]o value. The development of contractile tension in the atrial and ventricular muscles at various [Ca]o values corresponded well to these changes in the cellular calcium content except for the rat ventricle in which the contractile tension was almost proportional to [Ca]o. The relationship between the development of contractile tension and the level of cellular calcium content or [Ca]o was discussed."}
{"id": "PMID:1082045", "title": "[Surgical treatment of severe forms of ischemic heart disease].", "content": "Over 400 operations of an indirect revascularization of the myocardium have been performed at the A.I. Bakulev institute of Cardiovascular Surgery of the USSR Academy of Medical Sciences. Since 1970, the direct method have been developed, predominantly those of aorto-coronary bypass. Careful examination of 709 patients was carried out, 445 of them were subjected to selective coronary angiography, 108 were operated on. In 1974, 56 operations were undertaken, among them 36 aorto-coronary bypasses, in 6 of them with a preliminary endarterectomy from the coronary artery, 8 double bypasses, 6 procedures for acute myocardial infarction, 5 combined operations of aorto-coronary bypass and resections of cardiac aneurysms, and 15 resections of postinfarction aneurysms of the heart. Twelve patients died in the postoperative period, mainly due to acute cardiac insufficiency. The high figures of mortality are caused not only by the faults of the surgical procedures but also by the grave initial state of the patients (multiple coronary lesions large focal cardiosclerosis due to preceeding infarctions, high degree of initial cardiac insufficiency). The improvement of the results of surgery for the ischaemic heart disease will depend on the perfection of the surgical technique, on proper selection of the patients, and on the precise determination of the indications for such treatment.", "contents": "[Surgical treatment of severe forms of ischemic heart disease]. Over 400 operations of an indirect revascularization of the myocardium have been performed at the A.I. Bakulev institute of Cardiovascular Surgery of the USSR Academy of Medical Sciences. Since 1970, the direct method have been developed, predominantly those of aorto-coronary bypass. Careful examination of 709 patients was carried out, 445 of them were subjected to selective coronary angiography, 108 were operated on. In 1974, 56 operations were undertaken, among them 36 aorto-coronary bypasses, in 6 of them with a preliminary endarterectomy from the coronary artery, 8 double bypasses, 6 procedures for acute myocardial infarction, 5 combined operations of aorto-coronary bypass and resections of cardiac aneurysms, and 15 resections of postinfarction aneurysms of the heart. Twelve patients died in the postoperative period, mainly due to acute cardiac insufficiency. The high figures of mortality are caused not only by the faults of the surgical procedures but also by the grave initial state of the patients (multiple coronary lesions large focal cardiosclerosis due to preceeding infarctions, high degree of initial cardiac insufficiency). The improvement of the results of surgery for the ischaemic heart disease will depend on the perfection of the surgical technique, on proper selection of the patients, and on the precise determination of the indications for such treatment."}
{"id": "PMID:1082048", "title": "[Tritanopia and dominantly inherited optic atrophy (author's transl)].", "content": "The clinical picture of hereditary dominant optic atrophy is described and the particular importance of the concomitant tritan dyschromatopsia is stressed. Most cases of tritanopia are acquired, congenital ones are rare. Wright has studied 17 tritanopes - the greatest series ever reported. A full analysis of colour vision is given, but no detailed information about other visual functions and the condition of the fundi. As single members of  families affected by cominantly inherited optic atrophy show typical tritanopia, a visual acuity of 6/8 and hardly any pallor of their disks, it is possible that such isolated cases are regarded wrongly as congenital tritanopes.", "contents": "[Tritanopia and dominantly inherited optic atrophy (author's transl)]. The clinical picture of hereditary dominant optic atrophy is described and the particular importance of the concomitant tritan dyschromatopsia is stressed. Most cases of tritanopia are acquired, congenital ones are rare. Wright has studied 17 tritanopes - the greatest series ever reported. A full analysis of colour vision is given, but no detailed information about other visual functions and the condition of the fundi. As single members of  families affected by cominantly inherited optic atrophy show typical tritanopia, a visual acuity of 6/8 and hardly any pallor of their disks, it is possible that such isolated cases are regarded wrongly as congenital tritanopes."}
{"id": "PMID:1082051", "title": "Effect of tryptophan on polyriboadenylic acid and polyadenylic acid-messenger ribonucleic acid in rat liver.", "content": "The administration of tryptophan to fasted rats 1 hour before killing resulted in marked increases in the amounts of hepatic polyriboadenylic acid (poly(A)) and poly(a)-containing-mRNA in the cytoplasm and a shift in hepatic polyribosomes toward heavier aggregates. When animals were treated with cordycepin, or actinomycin D, to both, there was disaggregation of hepatic polyribosomes and inhibition of poly(A) synthesis. Administration of tryptophan to fasted animals pretreated with cordycepin, or actinomycin D, or both induced a shift in hepatic polyribosomes toward heavier aggregates and an increase in in vitro protein synthesis. Fasted rats that received [U-14C] adenosine to prelabel hepatic poly (A) and then were treated with cordycepin, or actinomycin D, or both before tubefeeding tryptophan revealed increased hepatic levels of labeled polyribosomal poly (A) in comparison with controls. Fasted rats that received 14C-orotic acid to prelabel poly (A)-mRNA and then were treated with actinomycin D after 1 hour and with tryptophan along with cycloheximide after 1 1/2 hours revealed marked increases in labeling of hepatic poly (A)-mRNA associated with polyribosomes in comparison with controls. These findings suggest that tryptophan may act to stimulate the transport of poly (A)-containing-mRNA into the cytoplasm of the liver.", "contents": "Effect of tryptophan on polyriboadenylic acid and polyadenylic acid-messenger ribonucleic acid in rat liver. The administration of tryptophan to fasted rats 1 hour before killing resulted in marked increases in the amounts of hepatic polyriboadenylic acid (poly(A)) and poly(a)-containing-mRNA in the cytoplasm and a shift in hepatic polyribosomes toward heavier aggregates. When animals were treated with cordycepin, or actinomycin D, to both, there was disaggregation of hepatic polyribosomes and inhibition of poly(A) synthesis. Administration of tryptophan to fasted animals pretreated with cordycepin, or actinomycin D, or both induced a shift in hepatic polyribosomes toward heavier aggregates and an increase in in vitro protein synthesis. Fasted rats that received [U-14C] adenosine to prelabel hepatic poly (A) and then were treated with cordycepin, or actinomycin D, or both before tubefeeding tryptophan revealed increased hepatic levels of labeled polyribosomal poly (A) in comparison with controls. Fasted rats that received 14C-orotic acid to prelabel poly (A)-mRNA and then were treated with actinomycin D after 1 hour and with tryptophan along with cycloheximide after 1 1/2 hours revealed marked increases in labeling of hepatic poly (A)-mRNA associated with polyribosomes in comparison with controls. These findings suggest that tryptophan may act to stimulate the transport of poly (A)-containing-mRNA into the cytoplasm of the liver."}
{"id": "PMID:1082049", "title": "[Effect of hypokinesia and varying microclimate on vestibular function].", "content": "The functional state of the vestibular system of the bed rested man is characterized by a decrease of the nystagmic parameters, increase of autonomic reactions and elongation of illusory sensations. This may reduce the static and kinetic stability of man to acceleration. The bed rest induced vestibular changes can be attributed to the functional changes in higher compartments of the central nervous system. The influence of an altered atmosphere on the vestibular function is insignificant.", "contents": "[Effect of hypokinesia and varying microclimate on vestibular function]. The functional state of the vestibular system of the bed rested man is characterized by a decrease of the nystagmic parameters, increase of autonomic reactions and elongation of illusory sensations. This may reduce the static and kinetic stability of man to acceleration. The bed rest induced vestibular changes can be attributed to the functional changes in higher compartments of the central nervous system. The influence of an altered atmosphere on the vestibular function is insignificant."}
{"id": "PMID:1082050", "title": "[Eye movements occurring during head rotation on artificial gravity stands].", "content": "On the basis of previously advanced theories the direction and pattern of eye movements induced by stimulation of semicircular canals onboard artificial gravity stations are predicted. The resulting data are important to describe expected disorientation illusions of vestibular origin as well as vestibular effects on the visual functions of tracking and fixation onboard artificial gravity stations.", "contents": "[Eye movements occurring during head rotation on artificial gravity stands]. On the basis of previously advanced theories the direction and pattern of eye movements induced by stimulation of semicircular canals onboard artificial gravity stations are predicted. The resulting data are important to describe expected disorientation illusions of vestibular origin as well as vestibular effects on the visual functions of tracking and fixation onboard artificial gravity stations."}
{"id": "PMID:1082054", "title": "Bedside hemodynamic monitoring. Its value in the diagnosis of tamponade complicating cardiac surgery.", "content": "Cardiac tamponade may be a difficult clinical diagnosis in the early postoperative period in patients undergoing open-hear surgery, particularly when the anterior or lateral pericardium is left open. Bedside monitoring of intracardiac pressures and determination of a \"pressure plateau\" between right atrial, right ventricular diastolic, pulmonary arterial diastolic, and pulmonary capillary wedge pressures are useful in the early diagnosis of cardiac tamponade. The value of such hemodynamic monitoring in the  diagnosis and treatment of cardiac tamponade in three patients with aorta-coronary artery bypass surgery in the early postoperative period is reported. Appropriate therapy, carried out on the basis of these studies, minimized the occurrence of further morbidity or possible death.", "contents": "Bedside hemodynamic monitoring. Its value in the diagnosis of tamponade complicating cardiac surgery. Cardiac tamponade may be a difficult clinical diagnosis in the early postoperative period in patients undergoing open-hear surgery, particularly when the anterior or lateral pericardium is left open. Bedside monitoring of intracardiac pressures and determination of a \"pressure plateau\" between right atrial, right ventricular diastolic, pulmonary arterial diastolic, and pulmonary capillary wedge pressures are useful in the early diagnosis of cardiac tamponade. The value of such hemodynamic monitoring in the  diagnosis and treatment of cardiac tamponade in three patients with aorta-coronary artery bypass surgery in the early postoperative period is reported. Appropriate therapy, carried out on the basis of these studies, minimized the occurrence of further morbidity or possible death."}
{"id": "PMID:1082055", "title": "[Diffuse immunological lung damage due to heterologous anti-thymocyte serum].", "content": "Heterologous anti-rat-thymocyte-serum produces an acute hemorrhagic pulmonary lesion. Pneumotoxicity is dependant upon complement since preparatory C' - depletion protects the experimental animal from its letal properties. Absorption of serum with finely dispersed homologous tissue suspensions of lung and thymus is eliminative of toxicity while prophylactic administration of histamin antagonists seems to be non-influential in the genesis of the pulmonary lesions.", "contents": "[Diffuse immunological lung damage due to heterologous anti-thymocyte serum]. Heterologous anti-rat-thymocyte-serum produces an acute hemorrhagic pulmonary lesion. Pneumotoxicity is dependant upon complement since preparatory C' - depletion protects the experimental animal from its letal properties. Absorption of serum with finely dispersed homologous tissue suspensions of lung and thymus is eliminative of toxicity while prophylactic administration of histamin antagonists seems to be non-influential in the genesis of the pulmonary lesions."}
{"id": "PMID:1082056", "title": "[Effect of active interferonization and anti-thymocyte globulin on the growth of virus-induced hamster melanoma A-Mel-S].", "content": "The present data show that biological and synthetic inducers of interferon are able to reduce the growth of a transplantable malignant melanoma in the Syrian hamster. The advantage of the biological inducer H-MP are that it is not toxic for humans and that a high titer of interferon can be maintained over a long period of time.", "contents": "[Effect of active interferonization and anti-thymocyte globulin on the growth of virus-induced hamster melanoma A-Mel-S]. The present data show that biological and synthetic inducers of interferon are able to reduce the growth of a transplantable malignant melanoma in the Syrian hamster. The advantage of the biological inducer H-MP are that it is not toxic for humans and that a high titer of interferon can be maintained over a long period of time."}
{"id": "PMID:1082057", "title": "[Effect of strophanthin on intestinal blood flow. A contribution to the physiopathology of hemorrhagic enteropathy].", "content": "The purpose of the present study was to compare in canine experiments hemodynamic and angiographic effects on mesenteric circulation. I. v. administration of strophanthin is leading to an increase of arterial pressure and a decrease of mesenteric blood flow. Most consistent angiographic changes are attenuation and local vasoconstriction of peripheral vessels. The relationship between digitalis administration and mesenteric underperfusion leading to non-occlusive mesenteric ischemia is discussed.", "contents": "[Effect of strophanthin on intestinal blood flow. A contribution to the physiopathology of hemorrhagic enteropathy]. The purpose of the present study was to compare in canine experiments hemodynamic and angiographic effects on mesenteric circulation. I. v. administration of strophanthin is leading to an increase of arterial pressure and a decrease of mesenteric blood flow. Most consistent angiographic changes are attenuation and local vasoconstriction of peripheral vessels. The relationship between digitalis administration and mesenteric underperfusion leading to non-occlusive mesenteric ischemia is discussed."}
{"id": "PMID:1082058", "title": "[Local gastric fibrinolysis activation].", "content": "Hemorrhagic gastric juice contains a higher fibrinolytic activity than normal gastric juice. It is liberated from damaged muco and possibly from hemolytic erythrocytes.", "contents": "[Local gastric fibrinolysis activation]. Hemorrhagic gastric juice contains a higher fibrinolytic activity than normal gastric juice. It is liberated from damaged muco and possibly from hemolytic erythrocytes."}
{"id": "PMID:1082059", "title": "[Effect of coronary bypass surgery on myocardial pressure and ventricular function in the dog].", "content": "In 7 dogs a severe stenosis of the left circumflex coronary artery (CCA) is produced in the course of 18 days. The influence of myocardial revascularization on left ventricular function and on intramyocardial pressures in the areas supplied by the CCA and by the left anterior descending coronary artery is studied. Neither under control conditions nor during acute left ventricular pressure load does opening a bypass graft between the carotid artery and CCA induce a significant change in total ventricular function (dp/dtmax, Vmax, left ventricular enddiastolic pressure). However, immediately after opening the graft intramyocardial pressure rises in the CCA area indicating a regional improvement of myocardial function.", "contents": "[Effect of coronary bypass surgery on myocardial pressure and ventricular function in the dog]. In 7 dogs a severe stenosis of the left circumflex coronary artery (CCA) is produced in the course of 18 days. The influence of myocardial revascularization on left ventricular function and on intramyocardial pressures in the areas supplied by the CCA and by the left anterior descending coronary artery is studied. Neither under control conditions nor during acute left ventricular pressure load does opening a bypass graft between the carotid artery and CCA induce a significant change in total ventricular function (dp/dtmax, Vmax, left ventricular enddiastolic pressure). However, immediately after opening the graft intramyocardial pressure rises in the CCA area indicating a regional improvement of myocardial function."}
{"id": "PMID:1082060", "title": "[Animal experiments on influencing ischemic myocardial damage following temporary coronary artery ligation].", "content": "Ischemic myocardial damage produced by temporary coronary artery occlusion of 60 min duration is diminished by beta-sympathicolytic acting TrasicorR (3 mg per kg) and osmotically cell-dehydrating Manitol (1 g per kg). Trasylol, a proteinases inhibiting substance, caused a favourable effect at very high doses (100 000 DIE per kg) only. The extent and irreversibility of myocardial ischemic damage was determined by experiments of 180 min duration by intravital fluorochromatisation with acrine orange, determination of hydrogen ion concentration at frozen section of heart muscle with pH-indicatorpaper and enzymehistochemical and histochemical methods.", "contents": "[Animal experiments on influencing ischemic myocardial damage following temporary coronary artery ligation]. Ischemic myocardial damage produced by temporary coronary artery occlusion of 60 min duration is diminished by beta-sympathicolytic acting TrasicorR (3 mg per kg) and osmotically cell-dehydrating Manitol (1 g per kg). Trasylol, a proteinases inhibiting substance, caused a favourable effect at very high doses (100 000 DIE per kg) only. The extent and irreversibility of myocardial ischemic damage was determined by experiments of 180 min duration by intravital fluorochromatisation with acrine orange, determination of hydrogen ion concentration at frozen section of heart muscle with pH-indicatorpaper and enzymehistochemical and histochemical methods."}
{"id": "PMID:1082061", "title": "[Immunotherapy of cancer: reality or hope? (AUTHOR'S TRANSL)].", "content": "The reality of immunotherapy of cancer is still in the trial stages. Supportive immunotherapy combined with conventional cancer therapy can prolong the duration of remission and perhaps raise the cure rate for many neoplastic diseases such as acute leukemias, sarcomas and malignant melanomas. Our knowledge of the immunologic reactions is still incomplete. We expect further progress  but we have no evidence that the immune response will be able to prevent or reverse all malignant growth.", "contents": "[Immunotherapy of cancer: reality or hope? (AUTHOR'S TRANSL)]. The reality of immunotherapy of cancer is still in the trial stages. Supportive immunotherapy combined with conventional cancer therapy can prolong the duration of remission and perhaps raise the cure rate for many neoplastic diseases such as acute leukemias, sarcomas and malignant melanomas. Our knowledge of the immunologic reactions is still incomplete. We expect further progress  but we have no evidence that the immune response will be able to prevent or reverse all malignant growth."}
{"id": "PMID:1082062", "title": "[Coronary heart disease: pathlogy (author's transl)].", "content": "The review deals first with the importance of the risk factors for coronary heart disease, the progression of coronary arteriosclerosis and the location of atherosclerotic lesions. The areas of blood supply to the coronary arteries and the effects of coronary insufficiency in the myocardium are discussed. Finally the aneurysm and the morphologic changes in aortocoronary venous bypass are described.", "contents": "[Coronary heart disease: pathlogy (author's transl)]. The review deals first with the importance of the risk factors for coronary heart disease, the progression of coronary arteriosclerosis and the location of atherosclerotic lesions. The areas of blood supply to the coronary arteries and the effects of coronary insufficiency in the myocardium are discussed. Finally the aneurysm and the morphologic changes in aortocoronary venous bypass are described."}
{"id": "PMID:1082063", "title": "[Coronary heart disease and bypass surgery: a clinical cardiologist's view (author's transl)].", "content": "The main goal of bypass surgery (BPS) is improvement of angina pectoris (AP), of performance of LV function, and of the prognosis of AP. Recently reported statistics indicate a significant improvement of the prognosis after BPS, especially in double or triple vessel disease. The main indication remains severe AP with significantly impaired performance, but prognostic aspects can also affect the decision of BPS. The area of regional ischemia should be determined by means of angiography during stress, and regional flow should be measured (scintigraphy etc.) to allow a more accurate definition of critical narrowings.", "contents": "[Coronary heart disease and bypass surgery: a clinical cardiologist's view (author's transl)]. The main goal of bypass surgery (BPS) is improvement of angina pectoris (AP), of performance of LV function, and of the prognosis of AP. Recently reported statistics indicate a significant improvement of the prognosis after BPS, especially in double or triple vessel disease. The main indication remains severe AP with significantly impaired performance, but prognostic aspects can also affect the decision of BPS. The area of regional ischemia should be determined by means of angiography during stress, and regional flow should be measured (scintigraphy etc.) to allow a more accurate definition of critical narrowings."}
{"id": "PMID:1082066", "title": "Core cooling after hypoxia and ischaemia.", "content": "Two patients sustained severe circulatory inadequacy before and during thoracotomy for coronary surgery. Both patients were promptly cote cooled to between 20 degrees and 25 degrees C. After coronary surgery, each patient was rewarmed and the operation was terminated uneventfully in each case, No important cerebral deficit was apparent despite periods of circulatory insufficiency and arrest which were well beyond the usually accepted limits. It is suggested that prompt core cooling may exert a protective effect in this context.", "contents": "Core cooling after hypoxia and ischaemia. Two patients sustained severe circulatory inadequacy before and during thoracotomy for coronary surgery. Both patients were promptly cote cooled to between 20 degrees and 25 degrees C. After coronary surgery, each patient was rewarmed and the operation was terminated uneventfully in each case, No important cerebral deficit was apparent despite periods of circulatory insufficiency and arrest which were well beyond the usually accepted limits. It is suggested that prompt core cooling may exert a protective effect in this context."}
{"id": "PMID:1082104", "title": "Pre- and postjunctional neuromuscular blockade by carbachol.", "content": "Carbachol, when applied to the bathing Ringer solution of frog sartorius muscles, caused depolarization of the endplate and a blockade of endplate potentials (EPP's), miniature EPP's (mepp's) and the iontophoretic acetylcholine potential. In muscles treated with an analog of hemicholinium-3, alpha, alpha'bis(dimethylammonium acetaldehyde diethylacetal)-p-p'-diacetylbiphenyl dibromide (DMAE), depolarization of the endplate by carbachol was blocked and the blockade by carbachol of the iontophoretic acetylcholine potential was prevented. These responses to carbachol were attributed to a postjunctional action that was antagonized by DMAE. In contrast, the blockade by carbachol of EPP's and mepp's was enhanced in DMAE-treated muscles at a time when carbachol-induced depolarization was blocked. This response to carbachol was attributed to a pre-junctional action. Carbachol either blocked transmitter release by a mechanism that was insensitive to DMAE or enhanced the prejunctional blocking actions of DMAE. Succinylcholine had actions similar to carbachol. DMAE prevented depolarization by succinylcholine but enhanced neuromuscular blockade by succinylcholine. SKF 525-A (beta-diethylaminoethyl diphenylpropylacetate hydrochloride), like DMAE, prevented depolarization but not transmission blockade caused by carbachol.", "contents": "Pre- and postjunctional neuromuscular blockade by carbachol. Carbachol, when applied to the bathing Ringer solution of frog sartorius muscles, caused depolarization of the endplate and a blockade of endplate potentials (EPP's), miniature EPP's (mepp's) and the iontophoretic acetylcholine potential. In muscles treated with an analog of hemicholinium-3, alpha, alpha'bis(dimethylammonium acetaldehyde diethylacetal)-p-p'-diacetylbiphenyl dibromide (DMAE), depolarization of the endplate by carbachol was blocked and the blockade by carbachol of the iontophoretic acetylcholine potential was prevented. These responses to carbachol were attributed to a postjunctional action that was antagonized by DMAE. In contrast, the blockade by carbachol of EPP's and mepp's was enhanced in DMAE-treated muscles at a time when carbachol-induced depolarization was blocked. This response to carbachol was attributed to a pre-junctional action. Carbachol either blocked transmitter release by a mechanism that was insensitive to DMAE or enhanced the prejunctional blocking actions of DMAE. Succinylcholine had actions similar to carbachol. DMAE prevented depolarization by succinylcholine but enhanced neuromuscular blockade by succinylcholine. SKF 525-A (beta-diethylaminoethyl diphenylpropylacetate hydrochloride), like DMAE, prevented depolarization but not transmission blockade caused by carbachol."}
{"id": "PMID:1082105", "title": "[Corticalization of 2 regions of the visual system in the evolution of vertebrates].", "content": "Data on the evolution of the visual system in the phylogenetic scale of vertebrates are reported. Visual projections in the telencephalon of cyclostomes (lampreys) and retinothalamo-telencephalic channel in elasmobranches (skates) are shown. In amphibians (frogs) and reptiles (turtles) the existence of two visual subsystems (retino-thalamo-telencephalic and retino-tecto-thalamo-telencephalic) is determined, both being overlapped partially at the level of n. geniculatus lateralis and totally in the telencephalon. In turtles earlier visual and tectal impulses towards the telencephalon proved to be relayed in n. geniculatus lateralis and later ones--in n. rotundus. In mammals (rats) visual tecto-cortical connections are shown to have one synaptic relay in thalamic structures since cortical potentials evoked by stimulation of the colliculus superior in rats posses a rather short latency. Significant latency shortening of tectal visual responses in monkeys, as compared with the latency of the respective potentials in turtles (chronic identical experiments), supports the idea of the progressive development of the tectal division of the visual system in the vertebrate phylogony. It is concluded that both divisions of the visual system--retino-thalamic and retino-tectal underwent corticalization at earlier evolutionary stages.", "contents": "[Corticalization of 2 regions of the visual system in the evolution of vertebrates]. Data on the evolution of the visual system in the phylogenetic scale of vertebrates are reported. Visual projections in the telencephalon of cyclostomes (lampreys) and retinothalamo-telencephalic channel in elasmobranches (skates) are shown. In amphibians (frogs) and reptiles (turtles) the existence of two visual subsystems (retino-thalamo-telencephalic and retino-tecto-thalamo-telencephalic) is determined, both being overlapped partially at the level of n. geniculatus lateralis and totally in the telencephalon. In turtles earlier visual and tectal impulses towards the telencephalon proved to be relayed in n. geniculatus lateralis and later ones--in n. rotundus. In mammals (rats) visual tecto-cortical connections are shown to have one synaptic relay in thalamic structures since cortical potentials evoked by stimulation of the colliculus superior in rats posses a rather short latency. Significant latency shortening of tectal visual responses in monkeys, as compared with the latency of the respective potentials in turtles (chronic identical experiments), supports the idea of the progressive development of the tectal division of the visual system in the vertebrate phylogony. It is concluded that both divisions of the visual system--retino-thalamic and retino-tectal underwent corticalization at earlier evolutionary stages."}
{"id": "PMID:1082106", "title": "[Changes in the receptive field of pigeon tectum neurons subjected to vestibular and acoustic stimulation].", "content": "The changes in the receptive fields of neurons in the deep layers of the tectum opticum were studied as induced by labyrinthine and sound stimulation. Variations in the size of the receptive field, the particular direction of sensitive cells movement, duration of the reaction and amount of impulses in it were found. The results obtained and data from the literature allow one to suggest an active role of tectum opticum in the transformation of signals from the retina depending on the kind of information from other sensory systems.", "contents": "[Changes in the receptive field of pigeon tectum neurons subjected to vestibular and acoustic stimulation]. The changes in the receptive fields of neurons in the deep layers of the tectum opticum were studied as induced by labyrinthine and sound stimulation. Variations in the size of the receptive field, the particular direction of sensitive cells movement, duration of the reaction and amount of impulses in it were found. The results obtained and data from the literature allow one to suggest an active role of tectum opticum in the transformation of signals from the retina depending on the kind of information from other sensory systems."}
{"id": "PMID:1082107", "title": "[A pharmacologic analysis of the slow potential arising in response to adequate stimulation of the frog olfactory bulb].", "content": "The nature of slow potential recorded in the frog olfactory bulb in response to natural stimulation was studied using application of different pharmacological substances. Three suppositions were checked up: 1) if the slow potential is of neuro-glial nature; 2) whether it reflects the primary afferent depolarization in the terminations of olfactory fibres which is responsible for the presynaptic inhibition in the olfactory bulb (as the dorsal root potential in the spinal cord); 3) if it reflects postsynaptic potentials. The slow potential was found to have many pharmacological properties similar to those of the dorsal root potential in the spinal cord. However, the slow potential appears to be more a complicated response and includes at least one more component--a depolarization of dendrites of unknown nature.", "contents": "[A pharmacologic analysis of the slow potential arising in response to adequate stimulation of the frog olfactory bulb]. The nature of slow potential recorded in the frog olfactory bulb in response to natural stimulation was studied using application of different pharmacological substances. Three suppositions were checked up: 1) if the slow potential is of neuro-glial nature; 2) whether it reflects the primary afferent depolarization in the terminations of olfactory fibres which is responsible for the presynaptic inhibition in the olfactory bulb (as the dorsal root potential in the spinal cord); 3) if it reflects postsynaptic potentials. The slow potential was found to have many pharmacological properties similar to those of the dorsal root potential in the spinal cord. However, the slow potential appears to be more a complicated response and includes at least one more component--a depolarization of dendrites of unknown nature."}
{"id": "PMID:1082109", "title": "Persisting intrapituitary recessus infundibuli.", "content": "X-ray examination of the intracranial ventricles with Dimer X made it possible to make the diagnosis of obstructive hydrocephalus due to a mass lesion of the midbrain and abnormal intrasellar recess of the third ventricle in a 34 year old female patient. Considering the location and ontogenetic and phylogenetic facts one can diagnose the second pathological finding as a persisting intrapituitary recessus infundibuli. A malformation due to inhibition during development is to be assumed.", "contents": "Persisting intrapituitary recessus infundibuli. X-ray examination of the intracranial ventricles with Dimer X made it possible to make the diagnosis of obstructive hydrocephalus due to a mass lesion of the midbrain and abnormal intrasellar recess of the third ventricle in a 34 year old female patient. Considering the location and ontogenetic and phylogenetic facts one can diagnose the second pathological finding as a persisting intrapituitary recessus infundibuli. A malformation due to inhibition during development is to be assumed."}
{"id": "PMID:1082111", "title": "[Diverticulosis of Meckel's diverticulum in childhodd. 1 year's experience].", "content": "12 cases of Meckel's diverticulosis, submitted to operation, are examined. In the 41% of the patients, the ascertained seat of the disease was the diverticulum. The following complications were observed: phlogosis, enterorrhagia due to ulcer and occlusion for invagination. Mortality was of 8.3%.", "contents": "[Diverticulosis of Meckel's diverticulum in childhodd. 1 year's experience]. 12 cases of Meckel's diverticulosis, submitted to operation, are examined. In the 41% of the patients, the ascertained seat of the disease was the diverticulum. The following complications were observed: phlogosis, enterorrhagia due to ulcer and occlusion for invagination. Mortality was of 8.3%."}
{"id": "PMID:1082113", "title": "Alpha-1-antitrypsin variants in New Zealand.", "content": "Twelve percent of a sample of New Zealand Europeans were found to have variant forms of alpha-1-antitrypsin. The distribution of different variants was similar to that found in other Northern European populations. Four percent were heterozygotes for the deficiency state (Z allele) which predisposes to both emphysema and cirrhosis. An initial survey of New Zealand Maoris suggests that although they have a lower overall incidence of variants, there is an increased frequency of the deficiency Z allele. This may be a contributory factor to the susceptibility of the Maori to respiratory and liver disease.", "contents": "Alpha-1-antitrypsin variants in New Zealand. Twelve percent of a sample of New Zealand Europeans were found to have variant forms of alpha-1-antitrypsin. The distribution of different variants was similar to that found in other Northern European populations. Four percent were heterozygotes for the deficiency state (Z allele) which predisposes to both emphysema and cirrhosis. An initial survey of New Zealand Maoris suggests that although they have a lower overall incidence of variants, there is an increased frequency of the deficiency Z allele. This may be a contributory factor to the susceptibility of the Maori to respiratory and liver disease."}
{"id": "PMID:1082114", "title": "Alpha-1-antitrypsin deficiency in a Southland family.", "content": "After diagnosis of an alpha-1-antitrypsin deficiency in a patient with emphysema, three complete generations of his family were screened for the defect. Half of the 42 subjects had a defective phenotype (18 were MZ, two were ZZ, and one SZ). Of this group, five had other avoidable risk factors predisposing to emphysema, and a further 10 were children. The four oldest subjects had radiological evidence of emphysema. Patients at risk were advised to stop smoking and avoid occupational exposure to lung irritants.", "contents": "Alpha-1-antitrypsin deficiency in a Southland family. After diagnosis of an alpha-1-antitrypsin deficiency in a patient with emphysema, three complete generations of his family were screened for the defect. Half of the 42 subjects had a defective phenotype (18 were MZ, two were ZZ, and one SZ). Of this group, five had other avoidable risk factors predisposing to emphysema, and a further 10 were children. The four oldest subjects had radiological evidence of emphysema. Patients at risk were advised to stop smoking and avoid occupational exposure to lung irritants."}
{"id": "PMID:1082117", "title": "Mitogenic stimulation of lymphocytes in cancer patients.", "content": "Peripheral blood lymphocytes obtained from 16 controls, 16 untreated cancer patients and 15 cancer patients treated with irradiation were cultured with phytohemagglutinin (PHA) and pokeweed mitogen (PWM). The cultures were terminated on 3 and 7 days of incubation, and mitotic rates were obtained. The mitotic rates of untreated cancer patients for both mitogens did not differ significantly from those of controls. However, the mitotic rate obtained for 3-day PHA culture of the radiation-treated cancer patients was significantly different from controls, whereas the 7-day mitotic rate stimulated by PWM was significantly lower than the untreated cancer patients and controls.", "contents": "Mitogenic stimulation of lymphocytes in cancer patients. Peripheral blood lymphocytes obtained from 16 controls, 16 untreated cancer patients and 15 cancer patients treated with irradiation were cultured with phytohemagglutinin (PHA) and pokeweed mitogen (PWM). The cultures were terminated on 3 and 7 days of incubation, and mitotic rates were obtained. The mitotic rates of untreated cancer patients for both mitogens did not differ significantly from those of controls. However, the mitotic rate obtained for 3-day PHA culture of the radiation-treated cancer patients was significantly different from controls, whereas the 7-day mitotic rate stimulated by PWM was significantly lower than the untreated cancer patients and controls."}
{"id": "PMID:1082120", "title": "Liver in alpha1-antitrypsin deficiency: morphologic observations and in vitro synthesis of alpha1-antitrypsin.", "content": "In an effort to characterize the hepatic abnormality in patients with alpha1-antitrypsin deficiency, three unrelated children with the disorder (Pi types ZZ and SZ), two heterozygous parents (Pi type MZ), and three normal subjects (Pi type MM) were studied. As expected, the livers of the ZZ- and SZ-deficient subjects showed abnormal accumulation of alpha1-antitrypsin in the cisternae of the rough endoplasmic reticulum as judged by immunofluorescent and electron microscopic studies. Their parents (MZ phenotype) demonstrated identical although less extensive hepatic abnormalities. Short term cultures of liver tissue in the presence of radiolabeled amino acids showed both synthesis and release of alpha1-antitrypsin in normal control subjects and in the patients with the Z protein. Radiolabeled intracellular alpha1-antitrypsin could not be found. These studies demonstrate synthesis of alpha1-antitrypsin by the livers of normal and genetically deficient subjects in vitro, and suggest several possible mechanisms for alpha1-antitrypsin deficiency.", "contents": "Liver in alpha1-antitrypsin deficiency: morphologic observations and in vitro synthesis of alpha1-antitrypsin. In an effort to characterize the hepatic abnormality in patients with alpha1-antitrypsin deficiency, three unrelated children with the disorder (Pi types ZZ and SZ), two heterozygous parents (Pi type MZ), and three normal subjects (Pi type MM) were studied. As expected, the livers of the ZZ- and SZ-deficient subjects showed abnormal accumulation of alpha1-antitrypsin in the cisternae of the rough endoplasmic reticulum as judged by immunofluorescent and electron microscopic studies. Their parents (MZ phenotype) demonstrated identical although less extensive hepatic abnormalities. Short term cultures of liver tissue in the presence of radiolabeled amino acids showed both synthesis and release of alpha1-antitrypsin in normal control subjects and in the patients with the Z protein. Radiolabeled intracellular alpha1-antitrypsin could not be found. These studies demonstrate synthesis of alpha1-antitrypsin by the livers of normal and genetically deficient subjects in vitro, and suggest several possible mechanisms for alpha1-antitrypsin deficiency."}
{"id": "PMID:1082121", "title": "Morula forms of E rosettes: distribution, specific inhibition, and enhancement.", "content": "Rosetts-forming cells (RFC) with more than 10 sheep red blood cells (SRBC's) per cell defined as morula-forming cells (MFC) were studied in human lymphoid organs and in the peripheral blood (PBL). MFC are most common in the thymus (more than 50%) and the frequency is much less in peripheral lymphoid tissues and in the blood (29.8% in those less than 5 years old; 14.9% in adults). There is very slight decrease in total RFC in PBL over age 5, but the changes in MFC are quite striking. Treatment with antihuman thymus antiserum (ATS) and with anti-human lymphoblastic antiserum (ATL) resulted in significant inhibition of total rosette and morula rosette formation. No inhibition was seen using normal brain antiserum, anti-IgM antiserum, or anti-L chain antiserum. In two patients with chronic lymphocytic leukemia and in some cases of thymic deficiency the ratio of MFC to total RFC was unusually high. After culture with phytohemagglutinin (PHA) and conversion to blast forms or after surface peturbation by papain or freeze-thaw treatment, MFC numbers were increased.", "contents": "Morula forms of E rosettes: distribution, specific inhibition, and enhancement. Rosetts-forming cells (RFC) with more than 10 sheep red blood cells (SRBC's) per cell defined as morula-forming cells (MFC) were studied in human lymphoid organs and in the peripheral blood (PBL). MFC are most common in the thymus (more than 50%) and the frequency is much less in peripheral lymphoid tissues and in the blood (29.8% in those less than 5 years old; 14.9% in adults). There is very slight decrease in total RFC in PBL over age 5, but the changes in MFC are quite striking. Treatment with antihuman thymus antiserum (ATS) and with anti-human lymphoblastic antiserum (ATL) resulted in significant inhibition of total rosette and morula rosette formation. No inhibition was seen using normal brain antiserum, anti-IgM antiserum, or anti-L chain antiserum. In two patients with chronic lymphocytic leukemia and in some cases of thymic deficiency the ratio of MFC to total RFC was unusually high. After culture with phytohemagglutinin (PHA) and conversion to blast forms or after surface peturbation by papain or freeze-thaw treatment, MFC numbers were increased."}
{"id": "PMID:1082122", "title": "The significance of the Babinski sign in the newborn-a reappraisal.", "content": "A study of the clinical significance and the stimulus methods used to elicit the Babinski sign in normal newborns has been carried out. Although the most frequent response to cutaneous nociceptive stimulation of the lateral sole in neonates was extension of the great toe, a small but significant number of flexor responses were obtained. Consequently, the Babinski sign has limited clinical usefulness as a reflex indicating the presence or absence of neurological disease in the newborn. In reviewing previous studies in the literature, it was apparent that the widely differing percentages of extensor plantars reported in newborns was related directly to differences in the technique of examination.", "contents": "The significance of the Babinski sign in the newborn-a reappraisal. A study of the clinical significance and the stimulus methods used to elicit the Babinski sign in normal newborns has been carried out. Although the most frequent response to cutaneous nociceptive stimulation of the lateral sole in neonates was extension of the great toe, a small but significant number of flexor responses were obtained. Consequently, the Babinski sign has limited clinical usefulness as a reflex indicating the presence or absence of neurological disease in the newborn. In reviewing previous studies in the literature, it was apparent that the widely differing percentages of extensor plantars reported in newborns was related directly to differences in the technique of examination."}
{"id": "PMID:1082124", "title": "Feedback of isotonic muscle contraction on neuromuscular impulse transmission in the frog.", "content": "The effect of isotonic contraction on neuromuscular impulse transmission was studied in the in vivo M. gastrocnemius preparation of the frog. The N. ischiadicus was stimulated regularly with single pulses at different frequencies in the range of 1/8 to 8 Hz. When steady state conditions were reached, each half-minute a second stimulus was added to one of these pulses. The interval between the pulses of the pair was varied within the contraction cycle of the first stimulus. Compound extracellular or single intracellular action potentials were recorded from the muscle. At frequencies of 1/2-2 Hz a depression of the amplitude of the second compound muscle action potential of up to 50% of the first response was found when the muscle contracted isotonically. However, the second response was facilitated during an isometric contraction. The time course of the depression was equal to that of muscle shortening during the twitch, while the time course of facilitation corresponded roughly to that of facilitation of transmitter release. At lower frequencies the \"isotonic depression\" or \"isometric facilitation\" was not or only slightly present. However, at 1/8 Hz the depression could be evoked or increased by curarization. At frequencies higher than 2 Hz facilitation dominated over depression under isotonic conditions. With flexible intracellular micro-electrodes it was shown that the depression of the amplitude of the compound muscle action potential observed during the isotonic twitch was due to a reduction in neuromuscular impulse transmission. It is concluded that the isotonic depression is a negative feedback effect of the change of length of the contracting muscle on synaptic impulse transmission, probably due to an effect of length on transmitter release.", "contents": "Feedback of isotonic muscle contraction on neuromuscular impulse transmission in the frog. The effect of isotonic contraction on neuromuscular impulse transmission was studied in the in vivo M. gastrocnemius preparation of the frog. The N. ischiadicus was stimulated regularly with single pulses at different frequencies in the range of 1/8 to 8 Hz. When steady state conditions were reached, each half-minute a second stimulus was added to one of these pulses. The interval between the pulses of the pair was varied within the contraction cycle of the first stimulus. Compound extracellular or single intracellular action potentials were recorded from the muscle. At frequencies of 1/2-2 Hz a depression of the amplitude of the second compound muscle action potential of up to 50% of the first response was found when the muscle contracted isotonically. However, the second response was facilitated during an isometric contraction. The time course of the depression was equal to that of muscle shortening during the twitch, while the time course of facilitation corresponded roughly to that of facilitation of transmitter release. At lower frequencies the \"isotonic depression\" or \"isometric facilitation\" was not or only slightly present. However, at 1/8 Hz the depression could be evoked or increased by curarization. At frequencies higher than 2 Hz facilitation dominated over depression under isotonic conditions. With flexible intracellular micro-electrodes it was shown that the depression of the amplitude of the compound muscle action potential observed during the isotonic twitch was due to a reduction in neuromuscular impulse transmission. It is concluded that the isotonic depression is a negative feedback effect of the change of length of the contracting muscle on synaptic impulse transmission, probably due to an effect of length on transmitter release."}
{"id": "PMID:1082125", "title": "Recovery of sensitivity to acetylcholine following desensitization in muscles of different vertebrate species.", "content": "The recovery after desensitization (DS) of the frog sartorius muscle postsynaptic membrane has a constant rate with a half-time of about 6 sec at 22 degrees C. Several other avian and mammalian species were also examined to answer the question, whether this parameter differs in muscles of different function and acetylcholine receptor properties. The following muscles were studied using iontophoretic microapplication of ACh: frog m. cutaneus pectoris, chick anterior and posterior latissimus dorsi, innervated and denervated rat diaphragm, and diaphragm of awake and hibernating golden hamsters. In all cases, desensitization developed when series of 6-20 short ACh doses were applied to the end-plate region of the muscle fibre membrane without any DS potentiating drugs. Rate of DS onset was dependent on the dose and/or  frequency of ACh application. The rate of recovery, however, differs very little in all the muscles studied, the half-times being in the range of 5-6 sec at 22 degrees C. A similar rate of DS recovery was thus found in muscles with different pharmacological properties of cholinoreceptors (e.g. different sensitivity of frog and rat innervated and denervated muscles to d-tubocurarine), or different ionic pathways during activation (hibernating golden hamster). These results support the assumption that desensitization is not of receptor origin and indicate that the system operating during DS does not undergo substantial changes during the functional differentiation of vertebrate muscle membrane.", "contents": "Recovery of sensitivity to acetylcholine following desensitization in muscles of different vertebrate species. The recovery after desensitization (DS) of the frog sartorius muscle postsynaptic membrane has a constant rate with a half-time of about 6 sec at 22 degrees C. Several other avian and mammalian species were also examined to answer the question, whether this parameter differs in muscles of different function and acetylcholine receptor properties. The following muscles were studied using iontophoretic microapplication of ACh: frog m. cutaneus pectoris, chick anterior and posterior latissimus dorsi, innervated and denervated rat diaphragm, and diaphragm of awake and hibernating golden hamsters. In all cases, desensitization developed when series of 6-20 short ACh doses were applied to the end-plate region of the muscle fibre membrane without any DS potentiating drugs. Rate of DS onset was dependent on the dose and/or  frequency of ACh application. The rate of recovery, however, differs very little in all the muscles studied, the half-times being in the range of 5-6 sec at 22 degrees C. A similar rate of DS recovery was thus found in muscles with different pharmacological properties of cholinoreceptors (e.g. different sensitivity of frog and rat innervated and denervated muscles to d-tubocurarine), or different ionic pathways during activation (hibernating golden hamster). These results support the assumption that desensitization is not of receptor origin and indicate that the system operating during DS does not undergo substantial changes during the functional differentiation of vertebrate muscle membrane."}
{"id": "PMID:1082129", "title": "Effect of kinins on the central action of serotonin.", "content": "Kinins, the biologically active peptides, potentiate the central stimulatory effects of NA and depressant action of acetylcholine as have been shown by the authors previously. The aim of the work has been to study the effects of these peptides on the central action of 5-HT. For experiments female Wistar rats were used. 5-HT given ivc to the animals and 5-HTP applied ip resulted in an increase of the pain threshold to the electrical stimuli and in a worse motor coordination. Bradykinin injected ivc or kallikrein ip to some extent abolished these effects. The biochemical tests revealed that bradykinin ivc lead to a significant decrease in 5-HT content in the midbrain. In experiments in vitro this peptide increased markedly the release of the platelets 5-HT and inhibited the uptake of this mediator to platelets. The results have shown that kinins can attenuate the central action of 5-HT. This effect can be ascribed, at least in part to a kinin-induced changes of 5-HT concentration in some structures of the central nervous system.", "contents": "Effect of kinins on the central action of serotonin. Kinins, the biologically active peptides, potentiate the central stimulatory effects of NA and depressant action of acetylcholine as have been shown by the authors previously. The aim of the work has been to study the effects of these peptides on the central action of 5-HT. For experiments female Wistar rats were used. 5-HT given ivc to the animals and 5-HTP applied ip resulted in an increase of the pain threshold to the electrical stimuli and in a worse motor coordination. Bradykinin injected ivc or kallikrein ip to some extent abolished these effects. The biochemical tests revealed that bradykinin ivc lead to a significant decrease in 5-HT content in the midbrain. In experiments in vitro this peptide increased markedly the release of the platelets 5-HT and inhibited the uptake of this mediator to platelets. The results have shown that kinins can attenuate the central action of 5-HT. This effect can be ascribed, at least in part to a kinin-induced changes of 5-HT concentration in some structures of the central nervous system."}
{"id": "PMID:1082130", "title": "[Immunological status of patients with amebic hepatic abscess].", "content": "The authors studied 10 cases of amoebic hepatic abscess documented by clinical evidence and confirmed by laboratory tests, liver scan and a good response to treatment. The immunological state of the patients was determined by protein electrophoresis, immunoelectrophoresis, counter-immunoelectrophoresis, radial immunodiffusion and roset formation for T and B lymphocytes. It is concluded that the alterations of cellular and humoral immunity is evident in cases of amoebic hepatic abscess; this alterations are more clear in the acute form of the illness and the immunological deficiency is more significant in the celular immunity.", "contents": "[Immunological status of patients with amebic hepatic abscess]. The authors studied 10 cases of amoebic hepatic abscess documented by clinical evidence and confirmed by laboratory tests, liver scan and a good response to treatment. The immunological state of the patients was determined by protein electrophoresis, immunoelectrophoresis, counter-immunoelectrophoresis, radial immunodiffusion and roset formation for T and B lymphocytes. It is concluded that the alterations of cellular and humoral immunity is evident in cases of amoebic hepatic abscess; this alterations are more clear in the acute form of the illness and the immunological deficiency is more significant in the celular immunity."}
{"id": "PMID:1082135", "title": "Different responses of lymphoid cells from tumor-bearing as compared to tumor-immunized mice when sensitized to tumor specific antigens in vitro.", "content": "Lymphoid cells from spleen and lymph nodes of BALB/c mice were sensitized in vitro by cocultivation with cells from syngeneic methylcholanthrene-induced sarcomas. The donor mice were either immunized in vivo by tumor transplantation followed by excision (\"tumor-excised mice\") or were carrying a progressively growing tumor (\"tumor-bearing mice\"). The cell-mediated immune response of the sensitized cells was compared by a 36-hr microcytotoxicity assay. Lymphoid cells from tumor-excised mice were cytotoxic when tested after 3 days and also after 6 days of sensitization in vitro. Lymphoid cells from tumor-bearing mice were cytotoxic to cells of the tumor borne when tested after 3 days in culture. However, the cytotoxic effect was lost after 6 days of contact with the sensitizing tumor in vitro. Preliminary data suggest that the cultured lymphoid cells of tumor-bearing mice which have lost cytotoxicity, can suppress the reactivity of in vitro sensitized lymphoid cells from tumor-excised mice.", "contents": "Different responses of lymphoid cells from tumor-bearing as compared to tumor-immunized mice when sensitized to tumor specific antigens in vitro. Lymphoid cells from spleen and lymph nodes of BALB/c mice were sensitized in vitro by cocultivation with cells from syngeneic methylcholanthrene-induced sarcomas. The donor mice were either immunized in vivo by tumor transplantation followed by excision (\"tumor-excised mice\") or were carrying a progressively growing tumor (\"tumor-bearing mice\"). The cell-mediated immune response of the sensitized cells was compared by a 36-hr microcytotoxicity assay. Lymphoid cells from tumor-excised mice were cytotoxic when tested after 3 days and also after 6 days of sensitization in vitro. Lymphoid cells from tumor-bearing mice were cytotoxic to cells of the tumor borne when tested after 3 days in culture. However, the cytotoxic effect was lost after 6 days of contact with the sensitizing tumor in vitro. Preliminary data suggest that the cultured lymphoid cells of tumor-bearing mice which have lost cytotoxicity, can suppress the reactivity of in vitro sensitized lymphoid cells from tumor-excised mice."}
{"id": "PMID:1082136", "title": "Separation of T cell subpopulations capable of DNA synthesis, lymphotoxin release, and regulation of antigen and phytohemagglutinin responses on the basis of density and adherence properties.", "content": "T memory cells specifically responsive to ovalbumin and performing the diverse functions of DNA synthesis, lymphotoxin release, and regulation can be isolated in enriched numbers in the most buoyant fractions (A+B) of bovine serum albumin gradients on day 9 after sensitization. At least 20-30% of these cells are capable of mounting a blastogenic response to ovalbumin. A+B cells responding to ovalbumin with DNA synthesis have adherent properties and are further enriched on passage through glass wool. The subpopulations capable of entering into blastogenesis and DNA synthesis and of lymphotoxin release are unresponsive to T mitogens. A+B cells are capable of either potentiating or suppressing DNA synthetic responses to both phytohemagglutinin and antigen when added to 5 X 10(5) D cells in different proportions. Potentiation or suppression of phytohemagglutinin responses were observed with 1 X 10(5) A+B cells, and total suppression was observed with A+B in the range of 4 X 10(3) to 2 X 10(4). The response to antigen was sometimes inhibited in the same cell combinations that gave a potentiated response to phytohemagglutinin and vice versa. Regulatory cells in this system were not macrophages since their effect was not mimicked by addition of peritoneal macrophages, and ablation of macrophages by carrageenan affected neither the potentiation nor suppression.", "contents": "Separation of T cell subpopulations capable of DNA synthesis, lymphotoxin release, and regulation of antigen and phytohemagglutinin responses on the basis of density and adherence properties. T memory cells specifically responsive to ovalbumin and performing the diverse functions of DNA synthesis, lymphotoxin release, and regulation can be isolated in enriched numbers in the most buoyant fractions (A+B) of bovine serum albumin gradients on day 9 after sensitization. At least 20-30% of these cells are capable of mounting a blastogenic response to ovalbumin. A+B cells responding to ovalbumin with DNA synthesis have adherent properties and are further enriched on passage through glass wool. The subpopulations capable of entering into blastogenesis and DNA synthesis and of lymphotoxin release are unresponsive to T mitogens. A+B cells are capable of either potentiating or suppressing DNA synthetic responses to both phytohemagglutinin and antigen when added to 5 X 10(5) D cells in different proportions. Potentiation or suppression of phytohemagglutinin responses were observed with 1 X 10(5) A+B cells, and total suppression was observed with A+B in the range of 4 X 10(3) to 2 X 10(4). The response to antigen was sometimes inhibited in the same cell combinations that gave a potentiated response to phytohemagglutinin and vice versa. Regulatory cells in this system were not macrophages since their effect was not mimicked by addition of peritoneal macrophages, and ablation of macrophages by carrageenan affected neither the potentiation nor suppression."}
{"id": "PMID:1082137", "title": "H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice.", "content": "Sensitized lymphocytes can transfer a state of delayed-type hypersensitivity to soluble protein antigens to naive mice only if donor and recipient share the I-A region of the H-2 gene complex. Identity at the K or D region is not essential. The restriction is unlikely to result from ineffective homing of the injected cells or from their early destruction. It is thought to reflect a requirement for an Ir-gene controlled mechanism which governs effective interaction between sensitized T lymphocytes and antigen presented on the surface of macrophages.", "contents": "H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice. Sensitized lymphocytes can transfer a state of delayed-type hypersensitivity to soluble protein antigens to naive mice only if donor and recipient share the I-A region of the H-2 gene complex. Identity at the K or D region is not essential. The restriction is unlikely to result from ineffective homing of the injected cells or from their early destruction. It is thought to reflect a requirement for an Ir-gene controlled mechanism which governs effective interaction between sensitized T lymphocytes and antigen presented on the surface of macrophages."}
{"id": "PMID:1082138", "title": "Specific human B lymphocyte alloantigens linked to HL-A.", "content": "Sera, previously found to react specifically with B lymphoid cultured cells, were tested on isolated T and B peripheral blood lymphocytes in a microcytotoxicity assay. Studies were performed on lymphocytes obtained from several large Amish families. The sera used in these studies were cytotoxic to peripheral blood, B lymphocytes, but not cytotoxic to T lymphocytes. The antigens detected followed the inheritance pattern of HL-A haplotypes. The strong linkage disequilibrium with HL-A antigens suggests that genes controlling the expression of B lymphocyte antigens are linked to genes controlling HL-A alloantigens.", "contents": "Specific human B lymphocyte alloantigens linked to HL-A. Sera, previously found to react specifically with B lymphoid cultured cells, were tested on isolated T and B peripheral blood lymphocytes in a microcytotoxicity assay. Studies were performed on lymphocytes obtained from several large Amish families. The sera used in these studies were cytotoxic to peripheral blood, B lymphocytes, but not cytotoxic to T lymphocytes. The antigens detected followed the inheritance pattern of HL-A haplotypes. The strong linkage disequilibrium with HL-A antigens suggests that genes controlling the expression of B lymphocyte antigens are linked to genes controlling HL-A alloantigens."}
{"id": "PMID:1082139", "title": "Factor-VIII-related antigen: multiple molecular forms in human plasma.", "content": "Analysis of Factor-VIII-related antigen (vWAg) in normal plasma by crossed immunoelectrophoresis reveals an asymmetric pattern indicative of molecular heterogeneity. This asymmetric pattern apparently results from the presence of multiple molecular forms of VWAg with distinct, though partially overlapping, mobilities on crossed immunoelectrophoresis. Evidence for this conclusion has been obtained by separating these forms, one from another, utilizing cryoprecipitation, agarose gel chromatography, and ion-exchange chromatography. Variation in the relative destribution of VWAg forms in liver disease and disseminated intravascular coagulation suggests that the processes which govern generation and/or breakdown of these forms can be altered by disease.", "contents": "Factor-VIII-related antigen: multiple molecular forms in human plasma. Analysis of Factor-VIII-related antigen (vWAg) in normal plasma by crossed immunoelectrophoresis reveals an asymmetric pattern indicative of molecular heterogeneity. This asymmetric pattern apparently results from the presence of multiple molecular forms of VWAg with distinct, though partially overlapping, mobilities on crossed immunoelectrophoresis. Evidence for this conclusion has been obtained by separating these forms, one from another, utilizing cryoprecipitation, agarose gel chromatography, and ion-exchange chromatography. Variation in the relative destribution of VWAg forms in liver disease and disseminated intravascular coagulation suggests that the processes which govern generation and/or breakdown of these forms can be altered by disease."}
{"id": "PMID:1082140", "title": "Inhibition of cell-mediated cytolysis of trinitrophenyl-derivatized target cells by alloantisera directed to the products of the K and D loci of the H-2 complex.", "content": "The role of the products of the K and D loci of the H-2 complex as target antigens for cytotoxic T cells generated to modified syngeneic cells has been investigated. Spleen cells, when cocultured with trinitrophenyl-derivatized syngeneic cells, generate cytolytic T cells that lyse most effectively tumor or spleen targets that are trinitrophenyl-derivatized and H-2 identical to the sensitizing cell. Cytolysis is inhibited by alloantisera to the K and/or D specificities of the target cells and by anti-dinitrophenyl antisera. Cytolic cells generated in this manner are also able to lyse, though less efficiently, trinitrophenyl-derivatized tumor and spleen targets that do not share products of the K and D loci with the sensitizing cell, and this cytolysis is also blocked by alloantisera directed to the H-2 serological specificities of such targets.", "contents": "Inhibition of cell-mediated cytolysis of trinitrophenyl-derivatized target cells by alloantisera directed to the products of the K and D loci of the H-2 complex. The role of the products of the K and D loci of the H-2 complex as target antigens for cytotoxic T cells generated to modified syngeneic cells has been investigated. Spleen cells, when cocultured with trinitrophenyl-derivatized syngeneic cells, generate cytolytic T cells that lyse most effectively tumor or spleen targets that are trinitrophenyl-derivatized and H-2 identical to the sensitizing cell. Cytolysis is inhibited by alloantisera to the K and/or D specificities of the target cells and by anti-dinitrophenyl antisera. Cytolic cells generated in this manner are also able to lyse, though less efficiently, trinitrophenyl-derivatized tumor and spleen targets that do not share products of the K and D loci with the sensitizing cell, and this cytolysis is also blocked by alloantisera directed to the H-2 serological specificities of such targets."}
{"id": "PMID:1082141", "title": "Inhibition of in vitro immune responses by antisera to H-2 or Ir gene products.", "content": "Heat aggregated IgG and antisera reacting with Ir-1 products (Ia antigens) inhibited the in vitro primary responses of mouse spleen cells to SRBC by 50-90% and depressed EA but not EAC rosette formation by 70-90%. These experiments suggest that alloantisera to the H-2 complex express at least part of their inhibitory properties by blocking the Fc receptor on B cells, and the data support the concept that certain Ia antigens and the Fc receptor are identical or closely associated. In addition, these studies showed that the immune response may be inhibited by antisera that react with H-2K and H-2D gene products.", "contents": "Inhibition of in vitro immune responses by antisera to H-2 or Ir gene products. Heat aggregated IgG and antisera reacting with Ir-1 products (Ia antigens) inhibited the in vitro primary responses of mouse spleen cells to SRBC by 50-90% and depressed EA but not EAC rosette formation by 70-90%. These experiments suggest that alloantisera to the H-2 complex express at least part of their inhibitory properties by blocking the Fc receptor on B cells, and the data support the concept that certain Ia antigens and the Fc receptor are identical or closely associated. In addition, these studies showed that the immune response may be inhibited by antisera that react with H-2K and H-2D gene products."}
{"id": "PMID:1082142", "title": "Colony stimulating factor (CSF) from human leukemic urine: affinity chromatography and isoelectric focusing.", "content": "Biological activities associated with colony-stimulating factor (CSF) from human leukemic urine were found to be selectively retained on an affinity adsorbent of Con A-Sepharose. Elution of activity was achieved using a linear gradient of eith alpha-methyl-D-mannopyranoside (alphaMM) or alpha-methyl-D-glucopyranoside (alphaMG), and resulted in significant increases in specific biological activity. Rechromatography of appropriate fractions indicated that retention of CSF activities was not artifactual. Pretreatment of the affinity matrix with alphaMM completely inhibited binding of CSF. Affinity chromatography of CSF on a Blue Dextran-Sepharose adsorbent was found to be an effective method for removing albumin, a major protein contaminant in urinary preparations. Treatment of CSF with neuraminidase had no effect on its in vitro activity; however, such treatment resulted in an increase in the isoelectric point of CSF from pH 3.5 to pH 4.7, as determined using both sucrose and polyacrylamide gel stabilized pH gradients. Relatively broad regions of biological activity were observed following isoelectric focusing of both neuraminidase-treated and untreated CSF, suggesting that activity was associated with a heterogeneous/polydisperse population of molecular species.", "contents": "Colony stimulating factor (CSF) from human leukemic urine: affinity chromatography and isoelectric focusing. Biological activities associated with colony-stimulating factor (CSF) from human leukemic urine were found to be selectively retained on an affinity adsorbent of Con A-Sepharose. Elution of activity was achieved using a linear gradient of eith alpha-methyl-D-mannopyranoside (alphaMM) or alpha-methyl-D-glucopyranoside (alphaMG), and resulted in significant increases in specific biological activity. Rechromatography of appropriate fractions indicated that retention of CSF activities was not artifactual. Pretreatment of the affinity matrix with alphaMM completely inhibited binding of CSF. Affinity chromatography of CSF on a Blue Dextran-Sepharose adsorbent was found to be an effective method for removing albumin, a major protein contaminant in urinary preparations. Treatment of CSF with neuraminidase had no effect on its in vitro activity; however, such treatment resulted in an increase in the isoelectric point of CSF from pH 3.5 to pH 4.7, as determined using both sucrose and polyacrylamide gel stabilized pH gradients. Relatively broad regions of biological activity were observed following isoelectric focusing of both neuraminidase-treated and untreated CSF, suggesting that activity was associated with a heterogeneous/polydisperse population of molecular species."}
{"id": "PMID:1082143", "title": "Activation of T-lymphocyte helper function by brief exposure to antigen-pulsed macrophages.", "content": "In this report we investigated the ability of macrophage-associated OVA to stimulate purified OVA-immune T-lymphocytes to produce nonspecific helper activity for antibody production. T-cell activation required only a brief contact between the antigen-pulsed macrophages and T-lymphocytes and was maximal after a 4-hr contact. T-cell activation occurred at 37 but not at 4 degrees C, indicating that the macrophages and/or lymphocytes must be metabolically active for efficient activation. In addition, we compared the ability of antigen-pulsed syngeneic or allogeneic macrophages to activate T-cells for helper function.", "contents": "Activation of T-lymphocyte helper function by brief exposure to antigen-pulsed macrophages. In this report we investigated the ability of macrophage-associated OVA to stimulate purified OVA-immune T-lymphocytes to produce nonspecific helper activity for antibody production. T-cell activation required only a brief contact between the antigen-pulsed macrophages and T-lymphocytes and was maximal after a 4-hr contact. T-cell activation occurred at 37 but not at 4 degrees C, indicating that the macrophages and/or lymphocytes must be metabolically active for efficient activation. In addition, we compared the ability of antigen-pulsed syngeneic or allogeneic macrophages to activate T-cells for helper function."}
{"id": "PMID:1082145", "title": "Control of the rat's sniffing behavior by response-independent and dependent schedules of reinforcing brain stimulation.", "content": "The rat's sniffing response occurs in continuous bursts, at approximately 5-11 Hz. In the present experiments, the analog signal from a thermo-couple probe in the nasal cavity was digitized to provide a discrete logic pulse, defining a sniff, and permitting on-line presentations of reinforcing brain stimulation contingent on momentary sniffing patterns. Schedules of reinforcer presentation included response-independent fixed interval (temporal conditioning), continuous reinforcement (CRF), and differential reinforcement of low rates (DRL 10 sec). Under temporal conditioning, bursts of sniffing were observed immediately after stimulation, and an acceleration in sniffing developed preceding stimulation. Under CFR, operant rate-intensity functions were found to be similar to traditional bar-press data. Under DRL, sniffs were effectively paced by the criterion interval, and interresponse time analyses revealed evidence of temporal discrimination. The behavioral patterns were interpreted in terms of the interplay of operant and respondent functions.", "contents": "Control of the rat's sniffing behavior by response-independent and dependent schedules of reinforcing brain stimulation. The rat's sniffing response occurs in continuous bursts, at approximately 5-11 Hz. In the present experiments, the analog signal from a thermo-couple probe in the nasal cavity was digitized to provide a discrete logic pulse, defining a sniff, and permitting on-line presentations of reinforcing brain stimulation contingent on momentary sniffing patterns. Schedules of reinforcer presentation included response-independent fixed interval (temporal conditioning), continuous reinforcement (CRF), and differential reinforcement of low rates (DRL 10 sec). Under temporal conditioning, bursts of sniffing were observed immediately after stimulation, and an acceleration in sniffing developed preceding stimulation. Under CFR, operant rate-intensity functions were found to be similar to traditional bar-press data. Under DRL, sniffs were effectively paced by the criterion interval, and interresponse time analyses revealed evidence of temporal discrimination. The behavioral patterns were interpreted in terms of the interplay of operant and respondent functions."}
{"id": "PMID:1082146", "title": "Behavioral supersensitivity to 5-hydroxytryptophan induced by chronic methysergide pretreatment.", "content": "The administration of 5-hydroxytryptophan to intact guinea pigs results in rhythmic myoclonic behavior. This behavior is blocked acutely by methysergide, but is intensified in animals chronically pretreated with methysergide. Brain serotonin concentrations of guinea pigs pretreated with methysergide did not differ from saline-treated animals. These results are compatible with the hypothesis that prolonged methysergide administration can result in pharmacologically-induced denervation hypersensitivity at central serotonin receptors.", "contents": "Behavioral supersensitivity to 5-hydroxytryptophan induced by chronic methysergide pretreatment. The administration of 5-hydroxytryptophan to intact guinea pigs results in rhythmic myoclonic behavior. This behavior is blocked acutely by methysergide, but is intensified in animals chronically pretreated with methysergide. Brain serotonin concentrations of guinea pigs pretreated with methysergide did not differ from saline-treated animals. These results are compatible with the hypothesis that prolonged methysergide administration can result in pharmacologically-induced denervation hypersensitivity at central serotonin receptors."}
{"id": "PMID:1082144", "title": "Effects of phenylalanine and 5-hydroxytryptophan on seizure severity in mice.", "content": "The degree of audiogenic seizure was measured in DBA/2J (phenylalanine hydroxylase deficient) mice as a function of dietary phenylalanine (Phe) and injected 5-hydroxytryptophan (5-HTP), the precursor of serotonin (5-HT). Phe was shown to exacerbate seizures significantly, and seizure severity was found to be directly related to dietary concentration when animals were not treated with exogenous 5-HTP. 5-htp was observed to significantly ameliorate seizures. The seizure-intensifying effect of Phe was reversible by 5-HTP injection and protection against seizures was directly related to 5-HTP concentration for animals on a high Phe diet. The results of this study indicate that Phe and 5-HTP are mutually antagonistic in modulating audiogenic seizure suceptibility.", "contents": "Effects of phenylalanine and 5-hydroxytryptophan on seizure severity in mice. The degree of audiogenic seizure was measured in DBA/2J (phenylalanine hydroxylase deficient) mice as a function of dietary phenylalanine (Phe) and injected 5-hydroxytryptophan (5-HTP), the precursor of serotonin (5-HT). Phe was shown to exacerbate seizures significantly, and seizure severity was found to be directly related to dietary concentration when animals were not treated with exogenous 5-HTP. 5-htp was observed to significantly ameliorate seizures. The seizure-intensifying effect of Phe was reversible by 5-HTP injection and protection against seizures was directly related to 5-HTP concentration for animals on a high Phe diet. The results of this study indicate that Phe and 5-HTP are mutually antagonistic in modulating audiogenic seizure suceptibility."}
{"id": "PMID:1082148", "title": "Demonstration of bleeding from Meckel's diverticulum by means of selective arteriography of the superior mesenteric artery.", "content": "The diagnosis of a bleeding Meckel's diverticulum was made in a young man who presented several episodes of rectal bleeding over a period of 3 years. Attempted diagnosis by barium studies did not reveal the diverticulum. Angiography of the superior mesenteric artery revealed a wide and tortuous ileal branch -and at its distal end extravasation of contrast medium, within the walls of the diverticulum. It is suggested that in case of major bleeding from the lower GI tract, arteriography should be performed before other contrast medium studies.", "contents": "Demonstration of bleeding from Meckel's diverticulum by means of selective arteriography of the superior mesenteric artery. The diagnosis of a bleeding Meckel's diverticulum was made in a young man who presented several episodes of rectal bleeding over a period of 3 years. Attempted diagnosis by barium studies did not reveal the diverticulum. Angiography of the superior mesenteric artery revealed a wide and tortuous ileal branch -and at its distal end extravasation of contrast medium, within the walls of the diverticulum. It is suggested that in case of major bleeding from the lower GI tract, arteriography should be performed before other contrast medium studies."}
{"id": "PMID:1082149", "title": "Radiological diagnosis of intracranial processes in children.", "content": "In children with suspected brain tumours, clinical features and simple radiological procedures such as cranial X-rays give little information on the localization of the process. Even at the slightest suspicion of a tumour, extensive neuroradiological studies are indicated. The diagnostic importance of ventriculography, pneumoencephalography and angiography is discussed with reference to 28 patients.", "contents": "Radiological diagnosis of intracranial processes in children. In children with suspected brain tumours, clinical features and simple radiological procedures such as cranial X-rays give little information on the localization of the process. Even at the slightest suspicion of a tumour, extensive neuroradiological studies are indicated. The diagnostic importance of ventriculography, pneumoencephalography and angiography is discussed with reference to 28 patients."}
{"id": "PMID:1082150", "title": "Atypical radiographic features in Pneumocystis carinii pneumonia.", "content": "The chest films in 30 proved cases of Pneumocystis carinii pneumonia were reviewed to determine the incidence of atypical radiographic findings. Seventeen of 30 patients or 56% presented at least one atypical radiographic finding during the course of the disease. Unilateral distribution, lobar involvement, abscess formation, fulminant progression, and atelectatic changes were observed in more than half the patients. A classic radiographic presentation, justifying treatment without biopsy, was seen in only 13 of the 30 patients.", "contents": "Atypical radiographic features in Pneumocystis carinii pneumonia. The chest films in 30 proved cases of Pneumocystis carinii pneumonia were reviewed to determine the incidence of atypical radiographic findings. Seventeen of 30 patients or 56% presented at least one atypical radiographic finding during the course of the disease. Unilateral distribution, lobar involvement, abscess formation, fulminant progression, and atelectatic changes were observed in more than half the patients. A classic radiographic presentation, justifying treatment without biopsy, was seen in only 13 of the 30 patients."}
{"id": "PMID:1082163", "title": "[Deficiency of alpha-1-antitrypsin and the allele Pi nul].", "content": "Alpha-1-antitrypsin is a glycoprotein in human serum that inhibits several proteases. It is a polymorphic protein. A single autosomal locus (Pi), with multiple codominant alleles is responsible of the synthesis of alpha-1-antitrypsin. Of particular interest are alleles that lead to lower than normal concentrations of alpha-1-antitrypsin in serum, namely, PiS, PiP and PiZ. Some of these subjects carry a high risk of developing chronic obstructive pulmonary disease, especially when they are homozygotes for PiZ. In children, cirrhosis of the liver are also found in association with homozygosity for PiZ. recently, TALAMO discovered a subject whose serum contained no alpha-1-antitrypsin; this was the first case of total deficiency, and the patient carried a double dose of the so-called Pi--allele (Pi nul). We were able to demonstrate that a single dose of this allele exists in three families which we have studied in this paper. In a fourth family, the propositus carries Pi-- in duplicate. We report here the second case of the strange homozygous phenotype, Pi--. Surprisingly, we have found that alpha-1-antitrypsin is not completely absent in this serum; its concentration is 200 times lower than normal (less than 10 microgrammes per ml). At the moment, the existence of the Pi-- allele is obvious, but the significance of this small quantity of alpha-1-antitrypsin in the serum of such a patient remains unknown.", "contents": "[Deficiency of alpha-1-antitrypsin and the allele Pi nul]. Alpha-1-antitrypsin is a glycoprotein in human serum that inhibits several proteases. It is a polymorphic protein. A single autosomal locus (Pi), with multiple codominant alleles is responsible of the synthesis of alpha-1-antitrypsin. Of particular interest are alleles that lead to lower than normal concentrations of alpha-1-antitrypsin in serum, namely, PiS, PiP and PiZ. Some of these subjects carry a high risk of developing chronic obstructive pulmonary disease, especially when they are homozygotes for PiZ. In children, cirrhosis of the liver are also found in association with homozygosity for PiZ. recently, TALAMO discovered a subject whose serum contained no alpha-1-antitrypsin; this was the first case of total deficiency, and the patient carried a double dose of the so-called Pi--allele (Pi nul). We were able to demonstrate that a single dose of this allele exists in three families which we have studied in this paper. In a fourth family, the propositus carries Pi-- in duplicate. We report here the second case of the strange homozygous phenotype, Pi--. Surprisingly, we have found that alpha-1-antitrypsin is not completely absent in this serum; its concentration is 200 times lower than normal (less than 10 microgrammes per ml). At the moment, the existence of the Pi-- allele is obvious, but the significance of this small quantity of alpha-1-antitrypsin in the serum of such a patient remains unknown."}
{"id": "PMID:1082165", "title": "[Adhesion and aggregation in the subendothelium: hereditary platelet function disorder].", "content": "Platelet adhesion to a thrombogenic surface and adhesion-induced aggregation were investigated using a perfusion system at a blood flow rate similar to that observed in arteries. Morphometric measurements revealed diminished adhesion of platelets but normal surface-induced aggregation with blood of patients with von Willebrand's disease and with Bernard-Soulier syndrome. In contrast, surface-induced aggregation was defective with blood of patients with storage pool disease, thrombasthenia and with blood of healthy volunteers after Aspirin ingestion. These findings may explain the defective hemostasis in these patients. They suggest that platelet adhesion and aggregation are governed by different mechanisms.", "contents": "[Adhesion and aggregation in the subendothelium: hereditary platelet function disorder]. Platelet adhesion to a thrombogenic surface and adhesion-induced aggregation were investigated using a perfusion system at a blood flow rate similar to that observed in arteries. Morphometric measurements revealed diminished adhesion of platelets but normal surface-induced aggregation with blood of patients with von Willebrand's disease and with Bernard-Soulier syndrome. In contrast, surface-induced aggregation was defective with blood of patients with storage pool disease, thrombasthenia and with blood of healthy volunteers after Aspirin ingestion. These findings may explain the defective hemostasis in these patients. They suggest that platelet adhesion and aggregation are governed by different mechanisms."}
{"id": "PMID:1082166", "title": "What retains water in living cells?", "content": "Three types of evidence are presented showing that the retention of cell water does not necessarily depend on the possession of an intact cell membrane. The data agree with the concept that water retention in cells is due to multilayer adsorption on proteins and that the maintenance of the normal state of water relies on the presence of adenosine triphosphate as a cardinal adsorbent, controlling the protein conformations.", "contents": "What retains water in living cells? Three types of evidence are presented showing that the retention of cell water does not necessarily depend on the possession of an intact cell membrane. The data agree with the concept that water retention in cells is due to multilayer adsorption on proteins and that the maintenance of the normal state of water relies on the presence of adenosine triphosphate as a cardinal adsorbent, controlling the protein conformations."}
{"id": "PMID:1082167", "title": "Activation of transplant immunity: effect of donor leukocytes on thyroid allograft rejection.", "content": "The survival of thyroid allografts in mice was prolonged by either holding the grafts in vitro culture for 20 to 27 days or by cobalt-60 irradiation of the donor 2 days before transplantation  with or without the intravenous injection of colloidal carbon just before removing the thyroid from the donor. In both cases the rejection process was restored by an intravenous injection of recipients with living peritoneal exudate cells (50 to 80 percent macrophages) syngeneic to the thyroid donor.", "contents": "Activation of transplant immunity: effect of donor leukocytes on thyroid allograft rejection. The survival of thyroid allografts in mice was prolonged by either holding the grafts in vitro culture for 20 to 27 days or by cobalt-60 irradiation of the donor 2 days before transplantation  with or without the intravenous injection of colloidal carbon just before removing the thyroid from the donor. In both cases the rejection process was restored by an intravenous injection of recipients with living peritoneal exudate cells (50 to 80 percent macrophages) syngeneic to the thyroid donor."}
{"id": "PMID:1082168", "title": "Influence of cadmium and other trace metals on human alpha1-antitrypsin: an in vitro study.", "content": "The effect of trace metals on plasma alpha1-antitrypsin was studied in vitro by adding known concentrations of trace metals, either alone or in combination, to plasma. Cadmium was the only trace metal that reduced the concentration of alpha1-antitrypsin and depressed the trypsin inhibitory capacity. No such effects were found with divalent lead, mercury, nickel, iron, and zinc ions. The present study appears to offer a plausible explanation for the emphysema that occurs in industrial workers exposed to cadmium.", "contents": "Influence of cadmium and other trace metals on human alpha1-antitrypsin: an in vitro study. The effect of trace metals on plasma alpha1-antitrypsin was studied in vitro by adding known concentrations of trace metals, either alone or in combination, to plasma. Cadmium was the only trace metal that reduced the concentration of alpha1-antitrypsin and depressed the trypsin inhibitory capacity. No such effects were found with divalent lead, mercury, nickel, iron, and zinc ions. The present study appears to offer a plausible explanation for the emphysema that occurs in industrial workers exposed to cadmium."}
{"id": "PMID:1082169", "title": "Merocyanine 540 as an optical probe of transmembrane electrical activity in the heart.", "content": "Frog hearts stained with merocyanine 540 shows a 1.5 to 2.0 percent increase in fluorescence intensity at 585 nanometers during the cardiac action potential when excited with a 540-nonometer light beam. Fluorometric action potentials similar to those recorded with intracellular microelectrodes in pacemaker, atrial, and ventricular tissues were recorded by focusing a 1-millimeter excitation beam on various regions of the heart. The signal-to-noise ratio for a single action potential ranged between 10/1 and 40/1. In spontaneously pacing hearts the slower rate of rise of the fluorescence action potential is due to the slow propagation of the electrical signal. In solutions containing normal calcium concentrations the fluorometric signal is altered by contractions. Merocyanine 540 is biologically inert as it stains the cardiac cell membrane and acts as a sensitive optical probe of the change in transmembrane potential.", "contents": "Merocyanine 540 as an optical probe of transmembrane electrical activity in the heart. Frog hearts stained with merocyanine 540 shows a 1.5 to 2.0 percent increase in fluorescence intensity at 585 nanometers during the cardiac action potential when excited with a 540-nonometer light beam. Fluorometric action potentials similar to those recorded with intracellular microelectrodes in pacemaker, atrial, and ventricular tissues were recorded by focusing a 1-millimeter excitation beam on various regions of the heart. The signal-to-noise ratio for a single action potential ranged between 10/1 and 40/1. In spontaneously pacing hearts the slower rate of rise of the fluorescence action potential is due to the slow propagation of the electrical signal. In solutions containing normal calcium concentrations the fluorometric signal is altered by contractions. Merocyanine 540 is biologically inert as it stains the cardiac cell membrane and acts as a sensitive optical probe of the change in transmembrane potential."}
{"id": "PMID:1082170", "title": "Benign bone tumors.", "content": "There is little information in the literature concerning the role of bone scanning in benign bone neoplasms except for sporadic reports. Since the advent of 99mTc-polyphosphate, bone imaging has proven feasible and useful in locating the cause of bone pain, such as in osteoid osteomas, which are not always radiologically apparent, and in evaluating whether or not a radiologic lesion is indeed benign and solitary. Blood-pool images are particularly important in neoplastic disease, since the absence of hyperemia in the immediate postinjection period favors the diagnosis of a benign neoplasm, as does low-grade uptake on the delayed study. The scan, including pinhole magnification images, is especially valuable in diagnosing lesions in the spine and pelvis, which are poorly seen radiologically. We have studied various types of benign bone tumors, including simple and aneurysmal bone cysts, fibrous cortical defects, and nonossifying fibromas, all of which had minimal or no increased uptake of the radiopharmaceutical, unless traumatized. Although osteochondromas and enchondromas showed varied accumulation of activity, the scan was useful in differentiating these from sarcomatous lesions. All osteoid osteomas demonstrated marked activity, and could be accurately located preoperatively, as could the extent of fibrous dysplasia. The bone scan in the reticuloses also showed abnormal accumulation of activity, and aided in arriving at the prognosis and treatment of histiocytic bone lesions.", "contents": "Benign bone tumors. There is little information in the literature concerning the role of bone scanning in benign bone neoplasms except for sporadic reports. Since the advent of 99mTc-polyphosphate, bone imaging has proven feasible and useful in locating the cause of bone pain, such as in osteoid osteomas, which are not always radiologically apparent, and in evaluating whether or not a radiologic lesion is indeed benign and solitary. Blood-pool images are particularly important in neoplastic disease, since the absence of hyperemia in the immediate postinjection period favors the diagnosis of a benign neoplasm, as does low-grade uptake on the delayed study. The scan, including pinhole magnification images, is especially valuable in diagnosing lesions in the spine and pelvis, which are poorly seen radiologically. We have studied various types of benign bone tumors, including simple and aneurysmal bone cysts, fibrous cortical defects, and nonossifying fibromas, all of which had minimal or no increased uptake of the radiopharmaceutical, unless traumatized. Although osteochondromas and enchondromas showed varied accumulation of activity, the scan was useful in differentiating these from sarcomatous lesions. All osteoid osteomas demonstrated marked activity, and could be accurately located preoperatively, as could the extent of fibrous dysplasia. The bone scan in the reticuloses also showed abnormal accumulation of activity, and aided in arriving at the prognosis and treatment of histiocytic bone lesions."}
{"id": "PMID:1082173", "title": "Creation of a reliable gastric bleed in dogs for angiographic studies.", "content": "A method of creating a brisk and reliable bleed from the left gastric artery of the dog has been developed. This has been quantitated and emonstrated by celiac and left gastric arteriography. Such a model lends itself to studies on the pharmacologic or embolic control of bleeding of the stomach.", "contents": "Creation of a reliable gastric bleed in dogs for angiographic studies. A method of creating a brisk and reliable bleed from the left gastric artery of the dog has been developed. This has been quantitated and emonstrated by celiac and left gastric arteriography. Such a model lends itself to studies on the pharmacologic or embolic control of bleeding of the stomach."}
{"id": "PMID:1082174", "title": "Intestinal fistula complicating pancreatic abscess.", "content": "Intestinal fistulization following acute pancreatitis is a complication of abscess formation and may occur after initial surgical drainage. It should be suspected in anyone with protracted pancreatitis in whom an abdominal mass suddenly disappears or in whom gastrointestinal bleeding develops. Although transient improvement may occur, decompression will often be incomplete and will usually be followed by recurrent sepsis or severe life threatening hemorrhage. For this reason, spontaneous fistulization into the intestine does not eliminate the need for adequate surgical drainage. With fistulas into the colon, drainage should be combined with proximal diverting colostomy. Some duodenal fistulas may respond to abscess drainage and intravenously administered hyperalimentation, while others may require drainage plus conversion from a side to an end fistula.", "contents": "Intestinal fistula complicating pancreatic abscess. Intestinal fistulization following acute pancreatitis is a complication of abscess formation and may occur after initial surgical drainage. It should be suspected in anyone with protracted pancreatitis in whom an abdominal mass suddenly disappears or in whom gastrointestinal bleeding develops. Although transient improvement may occur, decompression will often be incomplete and will usually be followed by recurrent sepsis or severe life threatening hemorrhage. For this reason, spontaneous fistulization into the intestine does not eliminate the need for adequate surgical drainage. With fistulas into the colon, drainage should be combined with proximal diverting colostomy. Some duodenal fistulas may respond to abscess drainage and intravenously administered hyperalimentation, while others may require drainage plus conversion from a side to an end fistula."}
{"id": "PMID:1082176", "title": "A simple test to determine the site of bleeding in the upper part of the gastrointestinal tract.", "content": "A noninvasive test has been developed to distinguish variceal bleeding from bleeding due to gastritis or ulcer disease. The test is based on the ability of shed blood to lower the pO2 of the gastric saline solution irrigant. It correctly predicted the site of bleeding in four of five patients with variceal hemorrhage and nine of nine patients with bleeding from gastritis or ulcers.", "contents": "A simple test to determine the site of bleeding in the upper part of the gastrointestinal tract. A noninvasive test has been developed to distinguish variceal bleeding from bleeding due to gastritis or ulcer disease. The test is based on the ability of shed blood to lower the pO2 of the gastric saline solution irrigant. It correctly predicted the site of bleeding in four of five patients with variceal hemorrhage and nine of nine patients with bleeding from gastritis or ulcers."}
{"id": "PMID:1082177", "title": "The liver as a privileged site for parathyroid alloimplanation in the rat.", "content": "It has been suggested that the liver suppresses the immune response to alloantigen. This hypothesis was tested utilizing parathyroid implantation into the liver and muscles of inbred rats. Recipients were parathyroidectomized and, after significant hypocalcemia, donor parathyroids from minor histoincompatible rats were implanted either in liver or in thigh hamstring. In one experimental group second-set grafting also was tested. Graft survivals were evaluated by (1) duration of functional response (DFR), the period of time during which the serum calcium level remained above the postparathyroidectomy baseline, (2) maximal response (MR), the proximity of the postimplantation calcium peak response to the normal preparathyroidectomy calcium level. Parathyroid implants survived significantly longer in the liver compared to the muscle, only when implanted across minor histocompatible barriers. It is suggested that the liver may contain an inhibitor, capable of suppressing the lymphocytes and thus suppressing immune response, which can be demonstrated when the histocompatibility barrier is weak.", "contents": "The liver as a privileged site for parathyroid alloimplanation in the rat. It has been suggested that the liver suppresses the immune response to alloantigen. This hypothesis was tested utilizing parathyroid implantation into the liver and muscles of inbred rats. Recipients were parathyroidectomized and, after significant hypocalcemia, donor parathyroids from minor histoincompatible rats were implanted either in liver or in thigh hamstring. In one experimental group second-set grafting also was tested. Graft survivals were evaluated by (1) duration of functional response (DFR), the period of time during which the serum calcium level remained above the postparathyroidectomy baseline, (2) maximal response (MR), the proximity of the postimplantation calcium peak response to the normal preparathyroidectomy calcium level. Parathyroid implants survived significantly longer in the liver compared to the muscle, only when implanted across minor histocompatible barriers. It is suggested that the liver may contain an inhibitor, capable of suppressing the lymphocytes and thus suppressing immune response, which can be demonstrated when the histocompatibility barrier is weak."}
{"id": "PMID:1082180", "title": "Clinical observations and their relationship to laboratory findings in the haemophilias.", "content": "Continuous observations in shcool terms have been made on the frequency of spontaneous bleeding episodes in a group of adolescent boys suffereing from haemophilia (39 cases), Christmas disease (9 cases), and von Willebrand's disease (2 cases). The observations suggest that in haemophilia other factors, besides the deficient factor VIII, affect the bleeding frequency. In the two boys with von Willebrand's disease, the lower level of factor VIII was associated with the milder symptoms; whereas in boys with Christmas disease clinical severity and laboratory results seemed to be correlated.", "contents": "Clinical observations and their relationship to laboratory findings in the haemophilias. Continuous observations in shcool terms have been made on the frequency of spontaneous bleeding episodes in a group of adolescent boys suffereing from haemophilia (39 cases), Christmas disease (9 cases), and von Willebrand's disease (2 cases). The observations suggest that in haemophilia other factors, besides the deficient factor VIII, affect the bleeding frequency. In the two boys with von Willebrand's disease, the lower level of factor VIII was associated with the milder symptoms; whereas in boys with Christmas disease clinical severity and laboratory results seemed to be correlated."}
{"id": "PMID:1082183", "title": "Analysis of 140 consecutive autopsy cases of cerebrovascular strokes in northern Japan.", "content": "On the autopsy findings of the 140 consecutive stroke cases, some characteristics of cerebrovascular diseases in this district were discussed. Predominance of intracranial hemorrhage (cerebral hemorrhage and subarachnoid hemorrhage) over cerebral infarction was still evident. Ruptured intracranial aneurysms were demonstrated in 98% of fatal subarachnoid hemorrhage. Compared with the autopsy data of other institutions, the age of death was generally low, the median age for cerebral hemorrhage, subarachnoid hemorrhage and cerebral infarction falling in the fourth, fifth, and sixth decade, respectively. Cerebellar hemorrhage was relatively frequent, while blood dyscrasias and other symptomatic hemorrhage constituted only a small part in contrast with the report of American authors. A dominant role of hypertension in causing strokes was concluded from both the heart weight and the clinical records.", "contents": "Analysis of 140 consecutive autopsy cases of cerebrovascular strokes in northern Japan. On the autopsy findings of the 140 consecutive stroke cases, some characteristics of cerebrovascular diseases in this district were discussed. Predominance of intracranial hemorrhage (cerebral hemorrhage and subarachnoid hemorrhage) over cerebral infarction was still evident. Ruptured intracranial aneurysms were demonstrated in 98% of fatal subarachnoid hemorrhage. Compared with the autopsy data of other institutions, the age of death was generally low, the median age for cerebral hemorrhage, subarachnoid hemorrhage and cerebral infarction falling in the fourth, fifth, and sixth decade, respectively. Cerebellar hemorrhage was relatively frequent, while blood dyscrasias and other symptomatic hemorrhage constituted only a small part in contrast with the report of American authors. A dominant role of hypertension in causing strokes was concluded from both the heart weight and the clinical records."}
{"id": "PMID:1082184", "title": "Enhancement of gustatory neural response to salts following adaptation of frog tongue to quinine-HCI.", "content": "After a frog tongue was adapted to 0.001 M quinine-HCl(Q-HCl), a change in the gustatory neural responses to salts was investigated. The initial phasic response to a variety of salt solutions such as 0.1 M NaCl, KCl, LiCl, MgCl2 and CaCl2 was greatly potentiated as a result of the Q-HCl adaptation. A weaker enhancement of the response to salts was observed after the tongue was adapted to deionized water, compared with the control response to salts during Ringer adaptation. Therefore, the Q-HCl-induced enhancement of salt responses is due to the summated effect of Q-HCl solute and water solvent. Concerning the enhancing mechanism of Q-HCl, it is postulated that the membrane potential of some salt-sensitive taste cells will be displaced in the hyperpolarizing direction during the Q-HCl adaptation, and that large depolarization, which may be related to the enhanced nerve response, will be produced by applying salts after Q-HCl.", "contents": "Enhancement of gustatory neural response to salts following adaptation of frog tongue to quinine-HCI. After a frog tongue was adapted to 0.001 M quinine-HCl(Q-HCl), a change in the gustatory neural responses to salts was investigated. The initial phasic response to a variety of salt solutions such as 0.1 M NaCl, KCl, LiCl, MgCl2 and CaCl2 was greatly potentiated as a result of the Q-HCl adaptation. A weaker enhancement of the response to salts was observed after the tongue was adapted to deionized water, compared with the control response to salts during Ringer adaptation. Therefore, the Q-HCl-induced enhancement of salt responses is due to the summated effect of Q-HCl solute and water solvent. Concerning the enhancing mechanism of Q-HCl, it is postulated that the membrane potential of some salt-sensitive taste cells will be displaced in the hyperpolarizing direction during the Q-HCl adaptation, and that large depolarization, which may be related to the enhanced nerve response, will be produced by applying salts after Q-HCl."}
{"id": "PMID:1082187", "title": "[Hemorrhages after stomatological-surgical interventions. Experiences with patients of the stomatological emergency service at the university stomatological clinic in Halle].", "content": "The author reports of experiences with 627 patients presenting with haermorrhage at the night emergency service of a university clinic of stomatology. Postoperative bleedings occurred mainly in the molar region. In 6 patients, an underlying disease until then unknown was recognized as a cause; a further 3 patients were under anticoagulant therapy which had not received the attention it deserved. Local surgical and medicamentous therapy is of special importance in the treatment of postoperative bleeding.", "contents": "[Hemorrhages after stomatological-surgical interventions. Experiences with patients of the stomatological emergency service at the university stomatological clinic in Halle]. The author reports of experiences with 627 patients presenting with haermorrhage at the night emergency service of a university clinic of stomatology. Postoperative bleedings occurred mainly in the molar region. In 6 patients, an underlying disease until then unknown was recognized as a cause; a further 3 patients were under anticoagulant therapy which had not received the attention it deserved. Local surgical and medicamentous therapy is of special importance in the treatment of postoperative bleeding."}
{"id": "PMID:1082188", "title": "Multiple target cell killing by the cytolytic T lymphocyte and the mechanism of cytotoxicity.", "content": "Previous reports have shown that one cytolytic T lymphocyte (CTL) can kill more than one allogeneic target cell, but have not established whether after its action on one or more target cells, each CTL can attack a second set of target cells at a later time. The experiments described here show that one CTL can kill more than six target cells, and they suggest that CTL can do this sequentially in time. Hence, the small number of specifically sensitized lymphocytes found in rejecting grafts could play an important role. Moreover, sequential killing would imply that CTL are not harmed during their interaction with specific target cells. If killing were effected by secretion of a nonspecific toxin, the killer cell would then have evolved some device for resisting its own toxin in order to survive. Whether or not this is the case has not yet been fully resolved.", "contents": "Multiple target cell killing by the cytolytic T lymphocyte and the mechanism of cytotoxicity. Previous reports have shown that one cytolytic T lymphocyte (CTL) can kill more than one allogeneic target cell, but have not established whether after its action on one or more target cells, each CTL can attack a second set of target cells at a later time. The experiments described here show that one CTL can kill more than six target cells, and they suggest that CTL can do this sequentially in time. Hence, the small number of specifically sensitized lymphocytes found in rejecting grafts could play an important role. Moreover, sequential killing would imply that CTL are not harmed during their interaction with specific target cells. If killing were effected by secretion of a nonspecific toxin, the killer cell would then have evolved some device for resisting its own toxin in order to survive. Whether or not this is the case has not yet been fully resolved."}
{"id": "PMID:1082192", "title": "Recurrent corneal granular dystrophy: a clinicopathologic study.", "content": "Recurrence of granular corneal dystrophy occurred in two patients following two successive penetrating corneal grafts. In the first patient, the deposits involved the central stroma of the donor cornea, almost to the level of Descemet's membrane. The second patient had predominantly subepithelial deposits in bilateral recurrences. Electron microscopy revealed rod-shaped, electron dense material typical of granular dystrophy in both patients.", "contents": "Recurrent corneal granular dystrophy: a clinicopathologic study. Recurrence of granular corneal dystrophy occurred in two patients following two successive penetrating corneal grafts. In the first patient, the deposits involved the central stroma of the donor cornea, almost to the level of Descemet's membrane. The second patient had predominantly subepithelial deposits in bilateral recurrences. Electron microscopy revealed rod-shaped, electron dense material typical of granular dystrophy in both patients."}
{"id": "PMID:1082190", "title": "[Chemotherapy of gestational chorionepithelioma].", "content": "The department of Gynecology and Obstetrics of Florence University reports the following findings for the chemotherapy of gestational choriocarcinoma during the period 1962-74. Sixteen cases, 11 of which were rated high risk cases, were given chemotherapy with MTX alone or in association with other cytotoxic agents. In the 12 surviving cases that may be considered the incidence of complete remission (from 4 to 13 years) is 67%. Of the 8 cases rated as cured 3 were high risk cases at the beginning of treatment. Intermittent chemotherapy with MTX only was given by slow intravenous injection at doses of 3 to 4 mg/kg for 24-48 h followed from the 48th h by folinic acid treatment for 1-2 days. This schedule is recommended because of its negligible toxicity as a replacemen for intramuscular MTX in 5-day courses, which carries a fairly high risk of toxicity.", "contents": "[Chemotherapy of gestational chorionepithelioma]. The department of Gynecology and Obstetrics of Florence University reports the following findings for the chemotherapy of gestational choriocarcinoma during the period 1962-74. Sixteen cases, 11 of which were rated high risk cases, were given chemotherapy with MTX alone or in association with other cytotoxic agents. In the 12 surviving cases that may be considered the incidence of complete remission (from 4 to 13 years) is 67%. Of the 8 cases rated as cured 3 were high risk cases at the beginning of treatment. Intermittent chemotherapy with MTX only was given by slow intravenous injection at doses of 3 to 4 mg/kg for 24-48 h followed from the 48th h by folinic acid treatment for 1-2 days. This schedule is recommended because of its negligible toxicity as a replacemen for intramuscular MTX in 5-day courses, which carries a fairly high risk of toxicity."}
{"id": "PMID:1082195", "title": "[Gastrointestinal hemorrhage as a complication of different diseases and conditions].", "content": "The author presents an analysis of the cause of bleeding in 37 patients and the genesis of development of erosions, acute ulcers of the mucous membrane of the stomach and duodenum (57 observations). Some recommendations on the diagnosis and prophylaxis of gastrointestinal hemorrhages are given.", "contents": "[Gastrointestinal hemorrhage as a complication of different diseases and conditions]. The author presents an analysis of the cause of bleeding in 37 patients and the genesis of development of erosions, acute ulcers of the mucous membrane of the stomach and duodenum (57 observations). Some recommendations on the diagnosis and prophylaxis of gastrointestinal hemorrhages are given."}
{"id": "PMID:1082196", "title": "[Profuse hemorrhage into the digestive tract in aortic diseases].", "content": "Four observations of the aortic aneurysm rupture with hemorrhage into the jejunum (2 cases), duodenum (1 case), esophagus (1 case) are reported. The causes of development of pathological changes in the aortic wall were as follows; atherosclerosis - in 3 patients, tuberculosis - in 1.", "contents": "[Profuse hemorrhage into the digestive tract in aortic diseases]. Four observations of the aortic aneurysm rupture with hemorrhage into the jejunum (2 cases), duodenum (1 case), esophagus (1 case) are reported. The causes of development of pathological changes in the aortic wall were as follows; atherosclerosis - in 3 patients, tuberculosis - in 1."}
{"id": "PMID:1082212", "title": "[Allergic gastric hemorrhage in a case of milk hypersensitivity].", "content": "In a 50-year-old patient who was repeatedly hospitalised on account of melaena and twice operated without finding an ulcer the cause of the haemorrhage was first unknown. In the sixth massive haemorrhage endoscopically was established a haemorrhagic erosive gastritis which underwent a spontaneous retrogression (gastroscopic control). Since the skin test on milk was positive, it was not thought of an allergic gastritis by milk and a provocation test was carried out twice which had a positive result (haemorrhagic erosive gastritis after instillation of milk into the stomach). With the publication of this case the attention shall be turned to the gastric haemorrhage caused by a nutritive allergy as well as to the diagnostic value of the simply producible provocation test.", "contents": "[Allergic gastric hemorrhage in a case of milk hypersensitivity]. In a 50-year-old patient who was repeatedly hospitalised on account of melaena and twice operated without finding an ulcer the cause of the haemorrhage was first unknown. In the sixth massive haemorrhage endoscopically was established a haemorrhagic erosive gastritis which underwent a spontaneous retrogression (gastroscopic control). Since the skin test on milk was positive, it was not thought of an allergic gastritis by milk and a provocation test was carried out twice which had a positive result (haemorrhagic erosive gastritis after instillation of milk into the stomach). With the publication of this case the attention shall be turned to the gastric haemorrhage caused by a nutritive allergy as well as to the diagnostic value of the simply producible provocation test."}
{"id": "PMID:1082214", "title": "[T- and B-lymphocytes in the so-called collagenoses].", "content": "Lymphocytes of peripheral blood of patients with systemic lupus erythematosus, scleroderma and dermatomyositis were investigated, and relatively, with regard to T- or B-cell characteristics. T-lymphocytes of most patients with SLE were decreased both absolutely. B-lymphocytes mostly showed only a relative increase. Successful immunosuppressive treatment usually accompanied by normalization of T-cell number and with decrease of B-cells. This differentiation of lymphocytes is an additional aid in the determination of activity of SLE and a useful index of immunosuppressive-cytostatic treatment.", "contents": "[T- and B-lymphocytes in the so-called collagenoses]. Lymphocytes of peripheral blood of patients with systemic lupus erythematosus, scleroderma and dermatomyositis were investigated, and relatively, with regard to T- or B-cell characteristics. T-lymphocytes of most patients with SLE were decreased both absolutely. B-lymphocytes mostly showed only a relative increase. Successful immunosuppressive treatment usually accompanied by normalization of T-cell number and with decrease of B-cells. This differentiation of lymphocytes is an additional aid in the determination of activity of SLE and a useful index of immunosuppressive-cytostatic treatment."}
{"id": "PMID:1082220", "title": "[Dynamics of the antismallpox antibodies detectable in the passive hemagglutination reaction with various animal immunization schemes].", "content": "Dynamics of accumulation and preservation of antibodies detectable in the PHAT (PHAT-AT) was studied on rabbits and guinea pigs with the use of various doses of the living inactivated virus and their combination in comparison with the virus-neutralizing antibodies, antihemagglutinins and precipitins. Accumulation of the virus-neutralizing antibodies did not coincide in time with the curve of the PHAT-AT accumulation; the titres of the virus-neutralizing antibodies were higher than the PHAT-AT titres. At the same time the percentage of seroconversions determined by PHAT was equal to 100 and the PHAT-AT level directly depended on the immunizing dose, the time of administration and the type of the antigen. On the basis of the data obtained PHAT could be recommended as a test for the assessment of the immunological efficacy of the smallpox vaccinations.", "contents": "[Dynamics of the antismallpox antibodies detectable in the passive hemagglutination reaction with various animal immunization schemes]. Dynamics of accumulation and preservation of antibodies detectable in the PHAT (PHAT-AT) was studied on rabbits and guinea pigs with the use of various doses of the living inactivated virus and their combination in comparison with the virus-neutralizing antibodies, antihemagglutinins and precipitins. Accumulation of the virus-neutralizing antibodies did not coincide in time with the curve of the PHAT-AT accumulation; the titres of the virus-neutralizing antibodies were higher than the PHAT-AT titres. At the same time the percentage of seroconversions determined by PHAT was equal to 100 and the PHAT-AT level directly depended on the immunizing dose, the time of administration and the type of the antigen. On the basis of the data obtained PHAT could be recommended as a test for the assessment of the immunological efficacy of the smallpox vaccinations."}
{"id": "PMID:1082221", "title": "Effects of thyroxine on T-cell counts and tumour cell rejection in mice.", "content": "In an attempt to study the effect of thyroxine on peripheral T-cell (thymus derived lymphocyte) counts or immunological functions, inbred C3H/He mice (8-10 weeks old) were injected subcutaneously with thyroxine for more than 3 months. After treatment for 3 months the mice were examined for peripheral T-cell counts, thymic incorporation of tritiated thymidine and rejection of tumour transplants. The number of T-cells was counted by the indirect immunoflourescence method using anti-thetaC3H serum after separation of lymphocytes on \"Ficoll-Conray\". It was revealed that the peripheral counts of both lymphocytes and T-cells were increased in the thyroxine treated group as compared with the control group, as was reported in the patients with Graves' disease. Thymic incorporation of tritiated thymidine was also found to be significantly increased in the thyroxine treated group. In addition, in order to study T-cell activity of the host, thyroxine treated and control mice were challenged with Ehrlich carcinoma cells at several concentrations (10(2), 10(4) and 2 x 10(6) per mouse). It was found that rejection of tumour transplants was significantly enhanced in the T-cell rich mice. Thus, it is possible that throxine affects peripheral T-cell counts and enhances immunological functions of the host.", "contents": "Effects of thyroxine on T-cell counts and tumour cell rejection in mice. In an attempt to study the effect of thyroxine on peripheral T-cell (thymus derived lymphocyte) counts or immunological functions, inbred C3H/He mice (8-10 weeks old) were injected subcutaneously with thyroxine for more than 3 months. After treatment for 3 months the mice were examined for peripheral T-cell counts, thymic incorporation of tritiated thymidine and rejection of tumour transplants. The number of T-cells was counted by the indirect immunoflourescence method using anti-thetaC3H serum after separation of lymphocytes on \"Ficoll-Conray\". It was revealed that the peripheral counts of both lymphocytes and T-cells were increased in the thyroxine treated group as compared with the control group, as was reported in the patients with Graves' disease. Thymic incorporation of tritiated thymidine was also found to be significantly increased in the thyroxine treated group. In addition, in order to study T-cell activity of the host, thyroxine treated and control mice were challenged with Ehrlich carcinoma cells at several concentrations (10(2), 10(4) and 2 x 10(6) per mouse). It was found that rejection of tumour transplants was significantly enhanced in the T-cell rich mice. Thus, it is possible that throxine affects peripheral T-cell counts and enhances immunological functions of the host."}
{"id": "PMID:1082224", "title": "Experimental studies on the nerve--sensory cell relationship during degeneration and regeneration in ampullar nerves of the frog labyrinth.", "content": "The degeneration and regeneration of afferent and efferent nerves to the crista ampullaris in the frog were studied after transsection of the ampullar nerves. In some experiments one or both rostral ampullar nerves were simply divided and left in place. In other experiments the ampullar nerves to the horizontal and to the anterior vertical crista were divided and the branch to the former was brought into contact with the distal stump of the anterior vertical ampullar nerve. Sensory cells in the labyrinth survived total denervation for 1-2 weeks without regular signs of ultrastructural damage. The afferent synaptic structures in these cells were often preserved in the denervated stage. The afferent nerve fibres degenerated earlier than the efferent ones. Both type of fibres reinnervated the vestibular epithelium after division of the ampullar nerve. The fibres from a horizontal ampullar nerve could reinnervate a denervated anterior vertical crista, resulting in misdirected reflexes. About 40 days after division both function and morphological organization had been restored to normal.", "contents": "Experimental studies on the nerve--sensory cell relationship during degeneration and regeneration in ampullar nerves of the frog labyrinth. The degeneration and regeneration of afferent and efferent nerves to the crista ampullaris in the frog were studied after transsection of the ampullar nerves. In some experiments one or both rostral ampullar nerves were simply divided and left in place. In other experiments the ampullar nerves to the horizontal and to the anterior vertical crista were divided and the branch to the former was brought into contact with the distal stump of the anterior vertical ampullar nerve. Sensory cells in the labyrinth survived total denervation for 1-2 weeks without regular signs of ultrastructural damage. The afferent synaptic structures in these cells were often preserved in the denervated stage. The afferent nerve fibres degenerated earlier than the efferent ones. Both type of fibres reinnervated the vestibular epithelium after division of the ampullar nerve. The fibres from a horizontal ampullar nerve could reinnervate a denervated anterior vertical crista, resulting in misdirected reflexes. About 40 days after division both function and morphological organization had been restored to normal."}
{"id": "PMID:1082227", "title": "[The influence of age on vestibular function].", "content": "Regular changes in vestibular responsitivity as a function of age are demonstrated with the maximum speed of the slow phase of caloric nystagmus. The clinical implication of this is that, when doing caloric tests, one should not switch for no reason from a once accepted temperature sequence, otherwise comparison with other tests becomes impossible. It rotation tests the best reactions are found in young people. The amplitude is a good parameter to detect age-factors in rotatory nystagmus.", "contents": "[The influence of age on vestibular function]. Regular changes in vestibular responsitivity as a function of age are demonstrated with the maximum speed of the slow phase of caloric nystagmus. The clinical implication of this is that, when doing caloric tests, one should not switch for no reason from a once accepted temperature sequence, otherwise comparison with other tests becomes impossible. It rotation tests the best reactions are found in young people. The amplitude is a good parameter to detect age-factors in rotatory nystagmus."}
{"id": "PMID:1082233", "title": "[Models and experimentation. Vestibular adaptation].", "content": "In 1961, we have given the experimental proof that, in the frog, the excited or inhited electrical activity of the ampullar nerve produced by a copular deflection of long duration, showed a decline which was attributed to an \"adaptation\" of peripheral origin. We suggested that the \"inverse phase\" was due to this adaptation. Since then, many workers have showed that such an adaptation was to be found in the induced nystagmus by prolonged angular acceleration. The mathematical models constructed to explain the vestibular function have to take in account this response decline to coincide with the evolution of the slow phase velocity of the nystagmus, during the prolonged deflexions of the cupula produced by the clinical vestibular rotatory and caloric tests.", "contents": "[Models and experimentation. Vestibular adaptation]. In 1961, we have given the experimental proof that, in the frog, the excited or inhited electrical activity of the ampullar nerve produced by a copular deflection of long duration, showed a decline which was attributed to an \"adaptation\" of peripheral origin. We suggested that the \"inverse phase\" was due to this adaptation. Since then, many workers have showed that such an adaptation was to be found in the induced nystagmus by prolonged angular acceleration. The mathematical models constructed to explain the vestibular function have to take in account this response decline to coincide with the evolution of the slow phase velocity of the nystagmus, during the prolonged deflexions of the cupula produced by the clinical vestibular rotatory and caloric tests."}
{"id": "PMID:1082235", "title": "Reversibility of effects of very hypotonic fluids on in vitro frog gastric mucosa: a functional and morphological study.", "content": "Functional and morphological properties of the in vitro frog gastric mucosa were studied during and after exposure to very hypotonic (approximately 25 mOs M) solutions. Within 20 min the acid secretory rate decreased to zero, but it returned to normal levels after isotonic fluids had been restored. The potential difference (PD) dropped within the first minutes after the exposure to hypotonic solutions, and became inverted. Following the return of isotonic conditions the PD increased to levels higher than in the controls. The electrical resistance increased about 10-fold during the hypotonic period, but decreased to near normal values when isotonic conditions were restored. By light and electron microscopy the cells of the hypotonic mucosae appeared greatly swollen, and the alterations were assessed by morphometric methods. The gland lumina were almost obliterated, and the lamina propria was reduced to about 63% of its former volume. After the return to isotonic conditions normal morphology was restores. It is conceivable that the great increase in resistance duroing the hypotonic period was caused by the occlusion of the gland lumina. Quantitative analyses of the Na, K, and Cl tissue concentrations indicated a large loss of these ions during the hypotonic state. Presumably the epithelial cells in the hypotonic mucosae avoid bursting by rapidly letting large numbers of ions exit, which results in a celulular osmolarity close to that of the bathing fluids.", "contents": "Reversibility of effects of very hypotonic fluids on in vitro frog gastric mucosa: a functional and morphological study. Functional and morphological properties of the in vitro frog gastric mucosa were studied during and after exposure to very hypotonic (approximately 25 mOs M) solutions. Within 20 min the acid secretory rate decreased to zero, but it returned to normal levels after isotonic fluids had been restored. The potential difference (PD) dropped within the first minutes after the exposure to hypotonic solutions, and became inverted. Following the return of isotonic conditions the PD increased to levels higher than in the controls. The electrical resistance increased about 10-fold during the hypotonic period, but decreased to near normal values when isotonic conditions were restored. By light and electron microscopy the cells of the hypotonic mucosae appeared greatly swollen, and the alterations were assessed by morphometric methods. The gland lumina were almost obliterated, and the lamina propria was reduced to about 63% of its former volume. After the return to isotonic conditions normal morphology was restores. It is conceivable that the great increase in resistance duroing the hypotonic period was caused by the occlusion of the gland lumina. Quantitative analyses of the Na, K, and Cl tissue concentrations indicated a large loss of these ions during the hypotonic state. Presumably the epithelial cells in the hypotonic mucosae avoid bursting by rapidly letting large numbers of ions exit, which results in a celulular osmolarity close to that of the bathing fluids."}
{"id": "PMID:1082237", "title": "Follow-up catheterization of patients with myocardial infarction during coronary artery bypass surgery.", "content": "Of 197 consecutive patients having aortocoronary bypass grafts over a 30 month period, 38 (19 per cent) had ECG evidence of myocardial infarction. The infarctions occurred more commonly in patients receiving multiple grafts. The infarctions were usually in areas supplied by grafted vessels. The infarctions occurred most often in the inferior wall, even when multiple vessels were grafted. Eleven patients with intraoperative infarction have had repeat postoperative coronary arteriograms. Seven had all grafts patent; three of these patients had hypokinesis of the infarcted wall. Four of the 11 patients had one or more occluded grafts; three of these patients had an area of hypokinesis. We conclude that intraoperative myocardial infarction is a common problem in aortocoronary bypass surgery and is not necessarily caused by graft occlusion.", "contents": "Follow-up catheterization of patients with myocardial infarction during coronary artery bypass surgery. Of 197 consecutive patients having aortocoronary bypass grafts over a 30 month period, 38 (19 per cent) had ECG evidence of myocardial infarction. The infarctions occurred more commonly in patients receiving multiple grafts. The infarctions were usually in areas supplied by grafted vessels. The infarctions occurred most often in the inferior wall, even when multiple vessels were grafted. Eleven patients with intraoperative infarction have had repeat postoperative coronary arteriograms. Seven had all grafts patent; three of these patients had hypokinesis of the infarcted wall. Four of the 11 patients had one or more occluded grafts; three of these patients had an area of hypokinesis. We conclude that intraoperative myocardial infarction is a common problem in aortocoronary bypass surgery and is not necessarily caused by graft occlusion."}
{"id": "PMID:1082238", "title": "The effect of massive doses of vitamin A on the signs of vitamin A deficiency in preschool children.", "content": "Marked seasonal variation in the prevalence of signs of vitamin A deficiency was found in the 2nd year of a continuing study of children age 0 to 4-1/2 years in a village in West Bengal, confirming results of a previous 18-month study. Administration of 200,000 IU of vitamin A every 4 months completely eliminated night blindness and prevented the development of new cases of Bitot's spot in a statistically significant number of children. The effectiveness of massive doses of vitamin A, administered at intervals of 4 months, as a short-term measure to fight the problem, was confirmed in this village. The study yielded additional evidence of the complex etiology of Bitot's spot, since alternate day dose of vitamin A in addition to massive therapy failed to eliminate these spots.", "contents": "The effect of massive doses of vitamin A on the signs of vitamin A deficiency in preschool children. Marked seasonal variation in the prevalence of signs of vitamin A deficiency was found in the 2nd year of a continuing study of children age 0 to 4-1/2 years in a village in West Bengal, confirming results of a previous 18-month study. Administration of 200,000 IU of vitamin A every 4 months completely eliminated night blindness and prevented the development of new cases of Bitot's spot in a statistically significant number of children. The effectiveness of massive doses of vitamin A, administered at intervals of 4 months, as a short-term measure to fight the problem, was confirmed in this village. The study yielded additional evidence of the complex etiology of Bitot's spot, since alternate day dose of vitamin A in addition to massive therapy failed to eliminate these spots."}
{"id": "PMID:1082239", "title": "Xerophthalmia and blindness in Northeast Brazil.", "content": "Dietary and biochemical data have indicated that hypovitaminosis A is a public health problem in Northeast Brazil. However, there are few reports regarding clinical signs of hypovitaminosis A. Therefore, an epidemiological study was designed to study this problem. The study as done was primarily a review of hospital records of xerophthalmia in each state with attention paid to other nutrition factors. It was found that xerophthalmia is a problem in Northeast Brazil and a cause of blindness in certain areas. However, the number varied greatly from some states to others. Around 1,000 preschool-age children were recorded as blind from vitamin A deficiency in a 1-year period. It was also noted that the peak incidence of xerophthalmia and blindness was around 1 year of age. The government of Brazil is taking urgent measures to combat this deficiency with such measures as the supplementation of sugar with vitamin A and the distribution of massive doses of vitamin A.", "contents": "Xerophthalmia and blindness in Northeast Brazil. Dietary and biochemical data have indicated that hypovitaminosis A is a public health problem in Northeast Brazil. However, there are few reports regarding clinical signs of hypovitaminosis A. Therefore, an epidemiological study was designed to study this problem. The study as done was primarily a review of hospital records of xerophthalmia in each state with attention paid to other nutrition factors. It was found that xerophthalmia is a problem in Northeast Brazil and a cause of blindness in certain areas. However, the number varied greatly from some states to others. Around 1,000 preschool-age children were recorded as blind from vitamin A deficiency in a 1-year period. It was also noted that the peak incidence of xerophthalmia and blindness was around 1 year of age. The government of Brazil is taking urgent measures to combat this deficiency with such measures as the supplementation of sugar with vitamin A and the distribution of massive doses of vitamin A."}
{"id": "PMID:1082240", "title": "SPLEEN/PATHOL.", "content": "Lymphocyte subpopulations were studied in the peripheral blood, lymph nodes, and spleens in a group of 17 patients with untreated Hodgkin's disease. In 12 of 15 cases, diminished absolute levels of T-lymphocytes in the peripheral blood were found; however, this was correlated with total lymphopenia. No direct relationship between \"T-lymphopenia\" and diminished cellular immunity, as measured by phytohemagglutinin and pokeweed mitogen blast transformation, and delayed cutaneous hypersensitivity was demonstrated. In eight lymph nodes involved histologically by Hodgkin's disease, a preponderance of T-lymphocytes was found when compared with a group of seven hyperplastic nodes (78.2 +/- 8.9% versus 54.5 +/- 11.0%, P is less than 0.01). These latter data appear to be consistent with the hypothesis that the pathogenesis of Hodgkin's disease involves a cell-mediated immune response to a neoplastic (antigenic) element.", "contents": "SPLEEN/PATHOL. Lymphocyte subpopulations were studied in the peripheral blood, lymph nodes, and spleens in a group of 17 patients with untreated Hodgkin's disease. In 12 of 15 cases, diminished absolute levels of T-lymphocytes in the peripheral blood were found; however, this was correlated with total lymphopenia. No direct relationship between \"T-lymphopenia\" and diminished cellular immunity, as measured by phytohemagglutinin and pokeweed mitogen blast transformation, and delayed cutaneous hypersensitivity was demonstrated. In eight lymph nodes involved histologically by Hodgkin's disease, a preponderance of T-lymphocytes was found when compared with a group of seven hyperplastic nodes (78.2 +/- 8.9% versus 54.5 +/- 11.0%, P is less than 0.01). These latter data appear to be consistent with the hypothesis that the pathogenesis of Hodgkin's disease involves a cell-mediated immune response to a neoplastic (antigenic) element."}
{"id": "PMID:1082242", "title": "Ultrastructural aspects of interactions of platelets with microcrystalline collagen.", "content": "Whole blood anticoagulated with EDTA was stirred with high concentrations of a microcrystalline bovine dermal collagen preparation in order to study the interactions of blood cells with collagen at the ultrastructural level. Blood from normal subjects and from patients congenitally deficient in Factors VIII or XII or with thrombasthenia or von Willebrands disease was used. In scanning and transmission electron microscopic studies with blood from normal subjects and patients, platelets were seen to adhere to collagen, develop cell surface undulations, form pseudopods, and undergo morphologic changes suggestive of the release reaction. Although thrombasthenic platelets adhered to collagen, pseudopods formed by these cells were remarkably angulated and nodular. Relatively few von Willebrands platelets adhered to collagen, but those platelets that did adhere underwent the usual sequence of morphologic changes.", "contents": "Ultrastructural aspects of interactions of platelets with microcrystalline collagen. Whole blood anticoagulated with EDTA was stirred with high concentrations of a microcrystalline bovine dermal collagen preparation in order to study the interactions of blood cells with collagen at the ultrastructural level. Blood from normal subjects and from patients congenitally deficient in Factors VIII or XII or with thrombasthenia or von Willebrands disease was used. In scanning and transmission electron microscopic studies with blood from normal subjects and patients, platelets were seen to adhere to collagen, develop cell surface undulations, form pseudopods, and undergo morphologic changes suggestive of the release reaction. Although thrombasthenic platelets adhered to collagen, pseudopods formed by these cells were remarkably angulated and nodular. Relatively few von Willebrands platelets adhered to collagen, but those platelets that did adhere underwent the usual sequence of morphologic changes."}
{"id": "PMID:1082243", "title": "The incidence of red-green colourblindness in the populations of Tripolitania, Cyrenaica and Fezzan in Libya, and of the Kikuyu, Kamba, Taita, Taveta and Luo tribes of Kenya.", "content": "The results of surveys of colourblindness carried out using the Ishihara test cards in Libya and Kenya, involving 384 and 504 individuals respectively, are reported. The Libyan samples, drawn from three geographically distinct regions of the country, are relatively homogeneous. The Kenyan samples, on the other hand, are heterogeneous, but in toto they display a markedly lower colourblindness percentage than do the Libyans. The Kenyan data are in broad accord with the other data available for sub-Saharan African populations. There are very few sets of comparative data available for North African populations, but the Libyan material displays a lower incidence of colourblindness than the values reported in European populations.", "contents": "The incidence of red-green colourblindness in the populations of Tripolitania, Cyrenaica and Fezzan in Libya, and of the Kikuyu, Kamba, Taita, Taveta and Luo tribes of Kenya. The results of surveys of colourblindness carried out using the Ishihara test cards in Libya and Kenya, involving 384 and 504 individuals respectively, are reported. The Libyan samples, drawn from three geographically distinct regions of the country, are relatively homogeneous. The Kenyan samples, on the other hand, are heterogeneous, but in toto they display a markedly lower colourblindness percentage than do the Libyans. The Kenyan data are in broad accord with the other data available for sub-Saharan African populations. There are very few sets of comparative data available for North African populations, but the Libyan material displays a lower incidence of colourblindness than the values reported in European populations."}
{"id": "PMID:1082244", "title": "Chloride balance in Rana pipiens.", "content": "Frogs kept in dilute solutions of Cl- maintain a steady state with respect to this ion. Chloride is exchanged at a rate of about 15 mumol 100 g-1 h-1 (47 nmol cm-2 h-1). Over 90% of the efflux is integumentary of which about 50% is diffusion of the total influx. The rest is carrier mediated, half of which is exchange diffusion and half active transport. The chloride transport system displays saturation kinetics and is inhibited by acetazolamide. Uptake of Cl- is not dependent on concomitant uptake of cations. Salt-depleted frogs accumulate Cl- from dilute KCl or choline chloride in exchange for an endogenous base, probably HCO-3. High bath concentrations of NaCl (greater than 5 mM) abolish the active uptake of Cl- and increase the passive permeability of the skin to Cl-.", "contents": "Chloride balance in Rana pipiens. Frogs kept in dilute solutions of Cl- maintain a steady state with respect to this ion. Chloride is exchanged at a rate of about 15 mumol 100 g-1 h-1 (47 nmol cm-2 h-1). Over 90% of the efflux is integumentary of which about 50% is diffusion of the total influx. The rest is carrier mediated, half of which is exchange diffusion and half active transport. The chloride transport system displays saturation kinetics and is inhibited by acetazolamide. Uptake of Cl- is not dependent on concomitant uptake of cations. Salt-depleted frogs accumulate Cl- from dilute KCl or choline chloride in exchange for an endogenous base, probably HCO-3. High bath concentrations of NaCl (greater than 5 mM) abolish the active uptake of Cl- and increase the passive permeability of the skin to Cl-."}
{"id": "PMID:1082245", "title": "Chloride transport across isolated skin of Rana pipiens.", "content": "The influx of Cl- across isolated frog skin bathed on the outside by 0.8 mM NaCl is about 100 nmol cm-2 h-1, which is approximately twice the Cl- influx in intact animals. The influx consists of diffusion (1%), exchange diffusion (38%), and active transport (60%). About 80% of the influx is independent of Na+ in the outer bath and is also independent of concomitant inward movement of cations. Chloride is exchanged for anions, probably HCO-3. The Cl- transport system is saturable; Vmax is about 200 nmol cm-2 h-1, and Ks is about 0.7 mM Cl-. High external concentrations of NaCl increase unidirectional fluxes of Cl- and urea, indicating a change in paracellular pathways. Active transport of Cl- is temperature sensitive (Q10 equals 2.68) and is inhibited by cyanide, dinitrophenol, iodoacetic acid, iodide, thiocyanate, and acetazolamide. The Na-independent component of JClin was unaffected by amiloride, ouabain, or eserine, all of which inhibit Na+ transport.", "contents": "Chloride transport across isolated skin of Rana pipiens. The influx of Cl- across isolated frog skin bathed on the outside by 0.8 mM NaCl is about 100 nmol cm-2 h-1, which is approximately twice the Cl- influx in intact animals. The influx consists of diffusion (1%), exchange diffusion (38%), and active transport (60%). About 80% of the influx is independent of Na+ in the outer bath and is also independent of concomitant inward movement of cations. Chloride is exchanged for anions, probably HCO-3. The Cl- transport system is saturable; Vmax is about 200 nmol cm-2 h-1, and Ks is about 0.7 mM Cl-. High external concentrations of NaCl increase unidirectional fluxes of Cl- and urea, indicating a change in paracellular pathways. Active transport of Cl- is temperature sensitive (Q10 equals 2.68) and is inhibited by cyanide, dinitrophenol, iodoacetic acid, iodide, thiocyanate, and acetazolamide. The Na-independent component of JClin was unaffected by amiloride, ouabain, or eserine, all of which inhibit Na+ transport."}
{"id": "PMID:1082246", "title": "Contractile repriming of single muscle fibers: inactivation and tubular diffusion.", "content": "Following  maximal potassium contractures of isolated single fibers from Rana pipiens, a brief recovery period is required to restore the ability to produce maximal tension by subsequent depolarization. At normal potassium concentration (2.5 mM), there is a minimal lag time of 5 s before any tension is produced by a second exposure to elevated K+; half-maximal tension can be produced in 13 s. The rate of repriming is directly related to the square of the fiber radius and is slowed by lowering external calcium concentration (1.5-0.4 mM). There is no significant difference in any of these respects between normal fibers and fibers dissected from semitendinosus muscles which had been chronically denervated for periods of up to 8 wk. Increased potassium concentration (5-20 mM) in the recovery solution decreased the steady-state repriming level, affecting denervated fibers more than controls. No repriming occurred at concentrations above 20 mM (-48 mV membrane potential.) At these intermediate concentrations, repriming was less complete in denervated muscle, reflecting the increased speed of delayed contractile inactivation. From the times to 50% repriming, which were proportional to the square of fiber radius, we estimated an upper limit for the diffusion coefficient for K+ in the transverse tubular system of 0.3 X 10(-6) cm2/s for both control and chronically denervated fibers.", "contents": "Contractile repriming of single muscle fibers: inactivation and tubular diffusion. Following  maximal potassium contractures of isolated single fibers from Rana pipiens, a brief recovery period is required to restore the ability to produce maximal tension by subsequent depolarization. At normal potassium concentration (2.5 mM), there is a minimal lag time of 5 s before any tension is produced by a second exposure to elevated K+; half-maximal tension can be produced in 13 s. The rate of repriming is directly related to the square of the fiber radius and is slowed by lowering external calcium concentration (1.5-0.4 mM). There is no significant difference in any of these respects between normal fibers and fibers dissected from semitendinosus muscles which had been chronically denervated for periods of up to 8 wk. Increased potassium concentration (5-20 mM) in the recovery solution decreased the steady-state repriming level, affecting denervated fibers more than controls. No repriming occurred at concentrations above 20 mM (-48 mV membrane potential.) At these intermediate concentrations, repriming was less complete in denervated muscle, reflecting the increased speed of delayed contractile inactivation. From the times to 50% repriming, which were proportional to the square of fiber radius, we estimated an upper limit for the diffusion coefficient for K+ in the transverse tubular system of 0.3 X 10(-6) cm2/s for both control and chronically denervated fibers."}
{"id": "PMID:1082247", "title": "Difference between endocardial and epicardial utilization of glycogen in the ischemic heart.", "content": "In the nonischemic canine left ventricle, levels of glycogen, glucose-6-phosphate (G6P), and lactate, and phosphorylase activity in the endocardial layers were higher than those in the epicardial layers, but the phosphocreatine (PCr) level in the endocardial layers was lower than that in the epicardial layers, and there were no differences in the adenosine triphosphate (ATP) level between the endo- and epicardial layers. Upon ligation of a small branch of the left descending coronary artery, levels of glycogen and PCr decreased, while those of G6P and lactate increased, and the activity of phosphorylase increased. The level of ATP was not affected by the coronary ligation. Thus the coronary ligation accelerated the glycogenolysis and glycolysis in the myocardium without affecting ATP level, and the acceleration in metabolism in the endocardial layers was more rapid and marked than that in the epicardial layers.", "contents": "Difference between endocardial and epicardial utilization of glycogen in the ischemic heart. In the nonischemic canine left ventricle, levels of glycogen, glucose-6-phosphate (G6P), and lactate, and phosphorylase activity in the endocardial layers were higher than those in the epicardial layers, but the phosphocreatine (PCr) level in the endocardial layers was lower than that in the epicardial layers, and there were no differences in the adenosine triphosphate (ATP) level between the endo- and epicardial layers. Upon ligation of a small branch of the left descending coronary artery, levels of glycogen and PCr decreased, while those of G6P and lactate increased, and the activity of phosphorylase increased. The level of ATP was not affected by the coronary ligation. Thus the coronary ligation accelerated the glycogenolysis and glycolysis in the myocardium without affecting ATP level, and the acceleration in metabolism in the endocardial layers was more rapid and marked than that in the epicardial layers."}
{"id": "PMID:1082248", "title": "The significance of the dural supply from the carotid siphon.", "content": "The dural branches from the cavernous portion of the internal carotid consists of the meningophypopseal trunk, the inferior cavernous, and the capsular arteries. These vessels have extensive anastomoses in and around the sellar area. These arteries are not usually demonstrated angiographically; therefore, when they are enlarged, a pathological lesion should be suspected. There are numerous lesions other than meningiomas associated with hypertrophy of these dural branches and the corresponding vessels involved depends on the anatomic location of the associated lesion.", "contents": "The significance of the dural supply from the carotid siphon. The dural branches from the cavernous portion of the internal carotid consists of the meningophypopseal trunk, the inferior cavernous, and the capsular arteries. These vessels have extensive anastomoses in and around the sellar area. These arteries are not usually demonstrated angiographically; therefore, when they are enlarged, a pathological lesion should be suspected. There are numerous lesions other than meningiomas associated with hypertrophy of these dural branches and the corresponding vessels involved depends on the anatomic location of the associated lesion."}
{"id": "PMID:1082249", "title": "Postoperative reflux gastritis.", "content": "The chief clinical features of forty-nine patients with the syndrome of reflux \"alkaline\" gastritis were epigastric pain, bilious vomiting, anemia, and the dumping syndrome. Separation of the symptoms of this syndrome from the symptoms of a multitude of other postgastrectomy syndromes is difficult, being complicated by a high incidence of emotional instability in these patients. Endoscopy remains the mainstay in diagnosis; among the characteristic endoscopic features are adherent mucus, edema, mucosal friability, and erosions, most severe on the gastric aspect of the stoma. The surgical treatment of choice is Roux-en-Y gastrojejunostomy accompanied by vagectomy.", "contents": "Postoperative reflux gastritis. The chief clinical features of forty-nine patients with the syndrome of reflux \"alkaline\" gastritis were epigastric pain, bilious vomiting, anemia, and the dumping syndrome. Separation of the symptoms of this syndrome from the symptoms of a multitude of other postgastrectomy syndromes is difficult, being complicated by a high incidence of emotional instability in these patients. Endoscopy remains the mainstay in diagnosis; among the characteristic endoscopic features are adherent mucus, edema, mucosal friability, and erosions, most severe on the gastric aspect of the stoma. The surgical treatment of choice is Roux-en-Y gastrojejunostomy accompanied by vagectomy."}
{"id": "PMID:1082250", "title": "Portal hypertension in infants and children with histiocytosis X.", "content": "Histiocytosis X describes a disease characterized by histiocytic infiltration of the reticuloendothelial system, skin, bones, and pituitary gland. The disseminated form frequently occurs in infants and children. Chemotherapy has significantly improved the prognosis in this disorder. Sixty-three per cent of survivors, however, have some residual disability related to fibrosis of tissues previously infiltrated by histiocytes. In instances of liver involvement, healing by fibrosis may result in cirrhosis with portal hypertension and bleeding esophageal varices. Clinical findings include hepatosplenomegaly, jaundice, ascites, hypoalbuminemia, prolonged prothrombin time, and Bromsulphalein retention. Histologic examination of the liver shows a characteristic dense \"macronodular\" periportal  cirrhotic pattern. Three children with portal hypertension and bleeding varices due to healed histiocytosis X were sucessfully managed by portosystemic shunt procedures. Portacaval, mesocaval, and central splenorenal shunts were equally effective in relieving poral hypertension. These children had neither recurrence of bleeding nor evidence of encephalopathy. Two children remain well whereas in one patient a primary hepatoma developed fourteen years posthung and he died of pulmonary metastases. Portosystemic shunt procedures effectively relieve the threat of potentially fatal variceal hemorrhage and improve the opportunity for long-term survival in children with cirrhosis and portal hypertension due to healed histiocytosis X.", "contents": "Portal hypertension in infants and children with histiocytosis X. Histiocytosis X describes a disease characterized by histiocytic infiltration of the reticuloendothelial system, skin, bones, and pituitary gland. The disseminated form frequently occurs in infants and children. Chemotherapy has significantly improved the prognosis in this disorder. Sixty-three per cent of survivors, however, have some residual disability related to fibrosis of tissues previously infiltrated by histiocytes. In instances of liver involvement, healing by fibrosis may result in cirrhosis with portal hypertension and bleeding esophageal varices. Clinical findings include hepatosplenomegaly, jaundice, ascites, hypoalbuminemia, prolonged prothrombin time, and Bromsulphalein retention. Histologic examination of the liver shows a characteristic dense \"macronodular\" periportal  cirrhotic pattern. Three children with portal hypertension and bleeding varices due to healed histiocytosis X were sucessfully managed by portosystemic shunt procedures. Portacaval, mesocaval, and central splenorenal shunts were equally effective in relieving poral hypertension. These children had neither recurrence of bleeding nor evidence of encephalopathy. Two children remain well whereas in one patient a primary hepatoma developed fourteen years posthung and he died of pulmonary metastases. Portosystemic shunt procedures effectively relieve the threat of potentially fatal variceal hemorrhage and improve the opportunity for long-term survival in children with cirrhosis and portal hypertension due to healed histiocytosis X."}
{"id": "PMID:1082253", "title": "Vitrectomy-instrumentation-system MS 7.", "content": "An instrumentation-system (MS 7) for open-sky- as well as for pars-plana-vitrectomy is described. The intraocular canula is equipped with a special rotating cutting device. Suction is generated by a suction-pump in the power supply unit. Intraocular illumination is obtained by a detachable light pipe on the vitrectomy-canula or-for the bimanual technique-by a special hand-piece with infusion and fiber optic illumination. The infusion-canula in the hand-piece can be replaced by a micro-scissor with infusion-channel. Despite the motor the whole system can be easy cleaned and sterilized.", "contents": "Vitrectomy-instrumentation-system MS 7. An instrumentation-system (MS 7) for open-sky- as well as for pars-plana-vitrectomy is described. The intraocular canula is equipped with a special rotating cutting device. Suction is generated by a suction-pump in the power supply unit. Intraocular illumination is obtained by a detachable light pipe on the vitrectomy-canula or-for the bimanual technique-by a special hand-piece with infusion and fiber optic illumination. The infusion-canula in the hand-piece can be replaced by a micro-scissor with infusion-channel. Despite the motor the whole system can be easy cleaned and sterilized."}
{"id": "PMID:1082254", "title": "Vitreoretinal juncture; healing of experimental wounds.", "content": "Healing of mechanically-induced, minimal wounds of vitreoretinal juncture in non-vascularized retina of rabbits was studied with electron microscopy at 1 and 3 days, and 1, 2, 6 and 10 weeks. Neuroectodermal scar was formed by two processes, each having a specific anatomical relationship to wound. Accessory gliocytosis, in which accessory glia adjacent to wound become phagocytic and proliferate; following this their progeny migrate to wound and progressively differentiate into fibrous astrocytes to fill in the wound proper. Plexiform gliosis, in which the M\u00fcller cell side branches proliferate to form a layer about the perimeter of wound. The retinal inner limiting lamina did not regenerate. The significance of these findings in relation to epiretinal membrane formation in man is discussed.", "contents": "Vitreoretinal juncture; healing of experimental wounds. Healing of mechanically-induced, minimal wounds of vitreoretinal juncture in non-vascularized retina of rabbits was studied with electron microscopy at 1 and 3 days, and 1, 2, 6 and 10 weeks. Neuroectodermal scar was formed by two processes, each having a specific anatomical relationship to wound. Accessory gliocytosis, in which accessory glia adjacent to wound become phagocytic and proliferate; following this their progeny migrate to wound and progressively differentiate into fibrous astrocytes to fill in the wound proper. Plexiform gliosis, in which the M\u00fcller cell side branches proliferate to form a layer about the perimeter of wound. The retinal inner limiting lamina did not regenerate. The significance of these findings in relation to epiretinal membrane formation in man is discussed."}
{"id": "PMID:1082255", "title": "Provoked iris ischaemia in the rabbit. I. Clinical and histopathological examinations.", "content": "Various surgical procedures were performed on albino rabbits, which impaired anterior segment blood circulation. Our early and late clinical observations showed a consequent development of anterior-segment ischaemia into anterior-segment necrosis. The significance of different surgical procedures for resulting complications is discussed. Histopathological observations showed ischaemic changes in the anterior segment: iris and ciliary body atrophy, cataract formation, rubeosis iridis. Even though changes in the anterior segment were not observed on biomicroscopic examination in the cases with ligation of one LPCA, histological studies revealed different degrees of sectoral iris atrophy with minor rubeosis.", "contents": "Provoked iris ischaemia in the rabbit. I. Clinical and histopathological examinations. Various surgical procedures were performed on albino rabbits, which impaired anterior segment blood circulation. Our early and late clinical observations showed a consequent development of anterior-segment ischaemia into anterior-segment necrosis. The significance of different surgical procedures for resulting complications is discussed. Histopathological observations showed ischaemic changes in the anterior segment: iris and ciliary body atrophy, cataract formation, rubeosis iridis. Even though changes in the anterior segment were not observed on biomicroscopic examination in the cases with ligation of one LPCA, histological studies revealed different degrees of sectoral iris atrophy with minor rubeosis."}
{"id": "PMID:1082256", "title": "An electron microscopic study on the blood-optic nerve and fluid-optic nerve barrier.", "content": "Utilizing horseradish peroxidase as a tracer, electron microscopic studies were done on the blood-optic nerve and fluid-optic nerve barrier to the peroxidase diffusion. Following intravenous injection the peroxidase was observed to fill the lumen of the capillaries of the laminar, prelaminar and orbital portions of the optic nerve but there was no penetratation of the capillary walls. The obstruction of the tracer diffusion out of capillary walls was attributed to the tight junctions between the endothelial cells. Peroxidase penetration was also absent in the capillaries of the pia and dura mater, however, was observed in pinocytotic vesicles of the endothelial cells. Lateral diffusion from the surrounding choroid into the optic nerve was detected but diffusion from the prelaminar optic nerve into the juxta-optic nerve retina was prevented by the Kuhnt intermediary tissue. Tight junctions which prevented peroxidase diffusion were found between the glial cells of the Kuhnt tissue, and this tissue was the barrier between the prelaminar optic nerve and the juxta-optic nerve retina. Peroxidase which was given into the lateral ventricle of the brain appeared in the subarachnoidal space around the optic nerve and penetrated freely into the optic nerve. The pial surface of the optic nerve possess no barrier activity. Peroxidase could be traced along the intercellular space between glial cells and optic nerve fibers. The basal lamina of the optic nerve capillaries was filled with peroxidase but diffusion into the capillary lumen was obstructured by the tight junctions between the endothelial cells.", "contents": "An electron microscopic study on the blood-optic nerve and fluid-optic nerve barrier. Utilizing horseradish peroxidase as a tracer, electron microscopic studies were done on the blood-optic nerve and fluid-optic nerve barrier to the peroxidase diffusion. Following intravenous injection the peroxidase was observed to fill the lumen of the capillaries of the laminar, prelaminar and orbital portions of the optic nerve but there was no penetratation of the capillary walls. The obstruction of the tracer diffusion out of capillary walls was attributed to the tight junctions between the endothelial cells. Peroxidase penetration was also absent in the capillaries of the pia and dura mater, however, was observed in pinocytotic vesicles of the endothelial cells. Lateral diffusion from the surrounding choroid into the optic nerve was detected but diffusion from the prelaminar optic nerve into the juxta-optic nerve retina was prevented by the Kuhnt intermediary tissue. Tight junctions which prevented peroxidase diffusion were found between the glial cells of the Kuhnt tissue, and this tissue was the barrier between the prelaminar optic nerve and the juxta-optic nerve retina. Peroxidase which was given into the lateral ventricle of the brain appeared in the subarachnoidal space around the optic nerve and penetrated freely into the optic nerve. The pial surface of the optic nerve possess no barrier activity. Peroxidase could be traced along the intercellular space between glial cells and optic nerve fibers. The basal lamina of the optic nerve capillaries was filled with peroxidase but diffusion into the capillary lumen was obstructured by the tight junctions between the endothelial cells."}
{"id": "PMID:1082257", "title": "[The effect of repeated adrenaline application on intraocular pressure and pupil in the rabbit (author's transl)].", "content": "The effect of single and repeated topical application of adrenaline on the intraocular pressure and pupil in the rabbit eye was studied using a 2% adrenaline borate and a commercial preparation of 2% adrenaline hydrochloride. On the first day, the hydrochloride gave a more pronounced pressure reduction and pupil diladation than the borate. This was found to be due to the benzalkonium chloride in the chloride preparation causing a better permeability of the adrenaline into the eye. From the second to the fifth day of adrenaline treatment a marked pressure decrease was found from the previous application of the day before. The following adrenaline application gave a hypertensive phase of intraocular pressure lasting two or three hours and a subsequent hypotensive phase lasting more than 24 hrs. The pupil diladation on the second and following days of treatment was not essentially different from the first day considering the observation period of two hours. The hypertensive phase could not be influenced by intravenous or intramuscular propranolol, however, the topical application of propranolol inhibited it completely. Some aspects of adrenergic regulation of the intraocular pressure are discussed.", "contents": "[The effect of repeated adrenaline application on intraocular pressure and pupil in the rabbit (author's transl)]. The effect of single and repeated topical application of adrenaline on the intraocular pressure and pupil in the rabbit eye was studied using a 2% adrenaline borate and a commercial preparation of 2% adrenaline hydrochloride. On the first day, the hydrochloride gave a more pronounced pressure reduction and pupil diladation than the borate. This was found to be due to the benzalkonium chloride in the chloride preparation causing a better permeability of the adrenaline into the eye. From the second to the fifth day of adrenaline treatment a marked pressure decrease was found from the previous application of the day before. The following adrenaline application gave a hypertensive phase of intraocular pressure lasting two or three hours and a subsequent hypotensive phase lasting more than 24 hrs. The pupil diladation on the second and following days of treatment was not essentially different from the first day considering the observation period of two hours. The hypertensive phase could not be influenced by intravenous or intramuscular propranolol, however, the topical application of propranolol inhibited it completely. Some aspects of adrenergic regulation of the intraocular pressure are discussed."}
{"id": "PMID:1082258", "title": "Intravitreal oxacillin in experimental staphylococcal endophthalmitis.", "content": "We compared combined intravitreal and systemic oxacillin to combined subconjunctival and systemic oxacillin in the treatment of experimental staphylococcal endophthalmitis. Intravitreal injection of 500 mcg was nontoxic to all intraocular structures and produced concentrations in the vitreous bactericidal to all susceptible strains of Staphylococcus aureus for almost 24 hours. In eyes treated 8, 10, and 12 hours after intravitreal inoculation of 10000 to 20000 penicillinase-producing S. aureus organisms, intravitreal injection of 500 mcg of oxacillin was superior to subconjunctival injection of 100 mg when each was combined with seven days of intensive intramuscular oxacillin.", "contents": "Intravitreal oxacillin in experimental staphylococcal endophthalmitis. We compared combined intravitreal and systemic oxacillin to combined subconjunctival and systemic oxacillin in the treatment of experimental staphylococcal endophthalmitis. Intravitreal injection of 500 mcg was nontoxic to all intraocular structures and produced concentrations in the vitreous bactericidal to all susceptible strains of Staphylococcus aureus for almost 24 hours. In eyes treated 8, 10, and 12 hours after intravitreal inoculation of 10000 to 20000 penicillinase-producing S. aureus organisms, intravitreal injection of 500 mcg of oxacillin was superior to subconjunctival injection of 100 mg when each was combined with seven days of intensive intramuscular oxacillin."}
{"id": "PMID:1082259", "title": "[Spectrophotometric studies for the demonstration of chromo-proteins and bilirubin in the eye (author's transl)].", "content": "Following intravenous injection we could demonstrate spectrophotometrically that in the rabbit chromo-proteins like hemoglobin and myoglobin as well as bilirubin are present in anterior chamber fluid, lens and vitreous body. We did not try yet to study the dynamics of these substances in relation to time and their quantitative distribution. Our findings, however, suggest a relationship between the concentration of these substances in the different parts of the eye and the serum. In lens, vitreous body and the anterior chamber fluid of patients dying with jaundice we also were able to demonstrate bilirubin spectrophotometrically.", "contents": "[Spectrophotometric studies for the demonstration of chromo-proteins and bilirubin in the eye (author's transl)]. Following intravenous injection we could demonstrate spectrophotometrically that in the rabbit chromo-proteins like hemoglobin and myoglobin as well as bilirubin are present in anterior chamber fluid, lens and vitreous body. We did not try yet to study the dynamics of these substances in relation to time and their quantitative distribution. Our findings, however, suggest a relationship between the concentration of these substances in the different parts of the eye and the serum. In lens, vitreous body and the anterior chamber fluid of patients dying with jaundice we also were able to demonstrate bilirubin spectrophotometrically."}
{"id": "PMID:1082260", "title": "[Dark adaptation in achromats (mathematical analysis) (author's transl)].", "content": "The integral dark adaptation curves of 10 complete acromats, 4 incomplete achromats and 11 normal subjects were compared using regression analysis. Neither in achromats nor in normal subjects a kink could be proven by this method. The final threshold of the mean dark adaptation curve of the complete achromats is slightly elevated. This may be explained by the fact that the group comprised 6 children.", "contents": "[Dark adaptation in achromats (mathematical analysis) (author's transl)]. The integral dark adaptation curves of 10 complete acromats, 4 incomplete achromats and 11 normal subjects were compared using regression analysis. Neither in achromats nor in normal subjects a kink could be proven by this method. The final threshold of the mean dark adaptation curve of the complete achromats is slightly elevated. This may be explained by the fact that the group comprised 6 children."}
{"id": "PMID:1082261", "title": "[Transport of 131J-iodo-o-hippurate and 22Na out of the retroretinal space in experimental non rhegmatogenous detachment of the retina (author's transl)].", "content": "A model of an experimental arhegmatogenous retinal detachment is presented, to allow the measurement of the transport of radioactive labeled substances out of the subretinal space. Experiments were performed on 19 male cats. 131I-iodo-o-hippurate and 22Na were used as test substances. There was no significant difference between the rate of disappearance of 131I-hippurate  if injected retroretinally or intravitreally. The rate of disappearance of 131I-hippurate could be slowed down by intravenous infusion of penicillin-G. The rate of disappearance  of 22Na following retroretinal injection is not only slower than the rate of disappearance of 22Na injected intravitreally, but significantly slower than the rate of disappearance of 131I-hippurate. For graphic analysis (\"curve peeling\") of the terms embodied in the curves, more densly situated points of measurements will be necessary. Then the experimental model will serve well to analyze the components influencing the transport of labeled sodium out of the  subretinal space.", "contents": "[Transport of 131J-iodo-o-hippurate and 22Na out of the retroretinal space in experimental non rhegmatogenous detachment of the retina (author's transl)]. A model of an experimental arhegmatogenous retinal detachment is presented, to allow the measurement of the transport of radioactive labeled substances out of the subretinal space. Experiments were performed on 19 male cats. 131I-iodo-o-hippurate and 22Na were used as test substances. There was no significant difference between the rate of disappearance of 131I-hippurate  if injected retroretinally or intravitreally. The rate of disappearance of 131I-hippurate could be slowed down by intravenous infusion of penicillin-G. The rate of disappearance  of 22Na following retroretinal injection is not only slower than the rate of disappearance of 22Na injected intravitreally, but significantly slower than the rate of disappearance of 131I-hippurate. For graphic analysis (\"curve peeling\") of the terms embodied in the curves, more densly situated points of measurements will be necessary. Then the experimental model will serve well to analyze the components influencing the transport of labeled sodium out of the  subretinal space."}
{"id": "PMID:1082262", "title": "[Influence of occlusion of retinal vessels on the transport of 22Na and 131J-iodo-o-hippurate out of the subretinal space (experiments performed on cats) (author's transl)].", "content": "By means of microsurgical technique and under the control of the ophthalmoscope 300 mul of isotone solution of NaCl with 22Na or o-131J-iodo-hippuric acid were injected into the space between the retina and pigment epithelium of 28 narcotized tomcats after having closed the inferior retina vessels by photocoagulation about 1 hr before in an umber of the animals. With 22Na we could demonstrate that the retinal vessels influence the speed of the transport of substances out of the space between retina and pigment epithelium: When the retina vessels are closed, the transport is decelerated. The same experiments with o-131J-iodo-hippuric acid did not show any difference between the eyes with coagulated vessels and those with open ones. We cannot answer the question, whether sodium is transported in a mono-directional flux out of the space between retina and pigment epithelium (probably followed by water), or if there is simply an exchange between 22Na out of this space with 22Na of the blood.", "contents": "[Influence of occlusion of retinal vessels on the transport of 22Na and 131J-iodo-o-hippurate out of the subretinal space (experiments performed on cats) (author's transl)]. By means of microsurgical technique and under the control of the ophthalmoscope 300 mul of isotone solution of NaCl with 22Na or o-131J-iodo-hippuric acid were injected into the space between the retina and pigment epithelium of 28 narcotized tomcats after having closed the inferior retina vessels by photocoagulation about 1 hr before in an umber of the animals. With 22Na we could demonstrate that the retinal vessels influence the speed of the transport of substances out of the space between retina and pigment epithelium: When the retina vessels are closed, the transport is decelerated. The same experiments with o-131J-iodo-hippuric acid did not show any difference between the eyes with coagulated vessels and those with open ones. We cannot answer the question, whether sodium is transported in a mono-directional flux out of the space between retina and pigment epithelium (probably followed by water), or if there is simply an exchange between 22Na out of this space with 22Na of the blood."}
{"id": "PMID:1082263", "title": "The influence of bretylium tosylate on the intraocular pressure of the rabbit.", "content": "Bretylium tosylate was applied topically and intravitreously to rabbit eyes. The topical application of 1, 2, 5 and 10% bretylium tosylate gave a small but significant decrease of the intraocular pressure 6 hrs after treatment. The effects had disappeared, when the eyes were re-examined after 24 hrs. The intravitreous injection of 200 mug of bretylium tosylate caused a more pronounced and prolonged pressure decrease. The results are interpreted in the light of chemical sympathectomy.", "contents": "The influence of bretylium tosylate on the intraocular pressure of the rabbit. Bretylium tosylate was applied topically and intravitreously to rabbit eyes. The topical application of 1, 2, 5 and 10% bretylium tosylate gave a small but significant decrease of the intraocular pressure 6 hrs after treatment. The effects had disappeared, when the eyes were re-examined after 24 hrs. The intravitreous injection of 200 mug of bretylium tosylate caused a more pronounced and prolonged pressure decrease. The results are interpreted in the light of chemical sympathectomy."}
{"id": "PMID:1082264", "title": "[Questions concerning the effect by diazepam (Faustan) in the EOG (author's transl)].", "content": "After oral medication of Faustan the EOG curve demonstrates: 1. a potential reduction throughout all 25 min of investigation; 2. an abnormal curve during dark time in several cases; 3. an inverse reaction in 7 persons.", "contents": "[Questions concerning the effect by diazepam (Faustan) in the EOG (author's transl)]. After oral medication of Faustan the EOG curve demonstrates: 1. a potential reduction throughout all 25 min of investigation; 2. an abnormal curve during dark time in several cases; 3. an inverse reaction in 7 persons."}
{"id": "PMID:1082265", "title": "[Acquired and congenital monocular pendular nystagmus. A comparative electronystagmographic study of two cases (author's transl)].", "content": "Comparative analysis of two cases of monocular pendular nystagmus (MPN). The nystagmus was acquired in one case of multiple sclerosis, and congenital in the other patient who had achromatopsia. A postsaccadic inhibition of 0.6-1 sec duration was seen only in the case of acquired MPN. All the other characteristics of the acquired and congenital monocular nystagmus were identical. The waveform of the nystagmus was sinusoidal, triangular or mixed. There was no modification of the MPN by eye position or by smooth pursuit. The nustagmus was inhibited by lid closure. Oscillopsia was reported by both patients. A peculiar disturbance of the sensorimotor feedback loop of the visual system is discussed as the possible common pathogenesis of acquired and congenital pendular nystagmus.", "contents": "[Acquired and congenital monocular pendular nystagmus. A comparative electronystagmographic study of two cases (author's transl)]. Comparative analysis of two cases of monocular pendular nystagmus (MPN). The nystagmus was acquired in one case of multiple sclerosis, and congenital in the other patient who had achromatopsia. A postsaccadic inhibition of 0.6-1 sec duration was seen only in the case of acquired MPN. All the other characteristics of the acquired and congenital monocular nystagmus were identical. The waveform of the nystagmus was sinusoidal, triangular or mixed. There was no modification of the MPN by eye position or by smooth pursuit. The nustagmus was inhibited by lid closure. Oscillopsia was reported by both patients. A peculiar disturbance of the sensorimotor feedback loop of the visual system is discussed as the possible common pathogenesis of acquired and congenital pendular nystagmus."}
{"id": "PMID:1082266", "title": "[Posttraumatic unilateral aphakia and contact lens -binocular functions of grown-ups].", "content": "The binocular results are only subnormal and comparable to former squinting patients. The results are better if one succeeds in reaching a visual acuity of at least 1.0. One can try to prevent a divergence by prescribing a contact lens as early as possible. Presbyopic patients have better binocular functions because the lost accommodation is the most interfering component (factor). It has disadvantageous effects especially on those patients who are yet presbyopic. The aniseikonia is in contrary to this not an as important interfering factor.", "contents": "[Posttraumatic unilateral aphakia and contact lens -binocular functions of grown-ups]. The binocular results are only subnormal and comparable to former squinting patients. The results are better if one succeeds in reaching a visual acuity of at least 1.0. One can try to prevent a divergence by prescribing a contact lens as early as possible. Presbyopic patients have better binocular functions because the lost accommodation is the most interfering component (factor). It has disadvantageous effects especially on those patients who are yet presbyopic. The aniseikonia is in contrary to this not an as important interfering factor."}
{"id": "PMID:1082267", "title": "[Fixation disparity under various test-conditions (author's transl)].", "content": "Measurements of fixation disparity and Panum areas were performed with a special device of the phase-difference haploscope in normal persons. There was a reproduceable influence on fixation disparity and size of Panum areas, when the conditions of examination were changed (position of the binocular fusion-pattern, brightness and contrast of the targets for sensory fusion and time of prism wearing). There found we found only under comparable conditions a quantitative relationship between the two phenomenons.", "contents": "[Fixation disparity under various test-conditions (author's transl)]. Measurements of fixation disparity and Panum areas were performed with a special device of the phase-difference haploscope in normal persons. There was a reproduceable influence on fixation disparity and size of Panum areas, when the conditions of examination were changed (position of the binocular fusion-pattern, brightness and contrast of the targets for sensory fusion and time of prism wearing). There found we found only under comparable conditions a quantitative relationship between the two phenomenons."}
{"id": "PMID:1082268", "title": "[Beginning atrophy of the eye bulb. An echographic-histologic comparative study (author's transl)].", "content": "In 21 cases of beginning atrophy of the eye bulb, ultrasongraphy revealed abnormal echoes reflected by equatorial and preequatorial parts of the bulbar shell. In addition, ultrasonography revealed a shortening of the bulbar axis by 0.88 mm and a thickening of the retroequatorial sclera up to 2.1 mm on an average. By correlation of the echogram and microscopic sections the presumption of an edema of the peripheral choroid and the ciliary body could be verified in 6 cases. Thus echography makes possible an earlier diagnosis of beginning bulbar atrophy than does the clinical symtomatology. In such cases surgical procedures for improvement of hypotony are useless or even detrimental.", "contents": "[Beginning atrophy of the eye bulb. An echographic-histologic comparative study (author's transl)]. In 21 cases of beginning atrophy of the eye bulb, ultrasongraphy revealed abnormal echoes reflected by equatorial and preequatorial parts of the bulbar shell. In addition, ultrasonography revealed a shortening of the bulbar axis by 0.88 mm and a thickening of the retroequatorial sclera up to 2.1 mm on an average. By correlation of the echogram and microscopic sections the presumption of an edema of the peripheral choroid and the ciliary body could be verified in 6 cases. Thus echography makes possible an earlier diagnosis of beginning bulbar atrophy than does the clinical symtomatology. In such cases surgical procedures for improvement of hypotony are useless or even detrimental."}
{"id": "PMID:1082269", "title": "[Clinical, light- and electron-microscopic investigations on the effect of 5-ethyl-2-deoxyuridine (EDU) in herpes simplex keratitis in rabbits (author's transl)].", "content": "Morphologic changes in rabbit cornea accompanying herpes simplex keratitis especially under the treatment of ethyldeoxyuridine (EDU) are summarized in regard to clinical aspects, light microscopy and electron microscopy. Untreated eyes show virus-dependent characteristic changes of the cell structure: peripheral migration of the cell chromatin, swelling of the nucleus, and disappearance of the nucleolus are persistent. In the nucleus as well as in the cytoplasm mature and immature virus particles are visible which demonstrate the normal virus-replication course. In the EDU treated cornea these particular changes are observed only in the primary stage. After prolonged treatment in the nucleus of the infected cells there are only immature virus particles with optically empty center. This can be evaluated as a sign of inhibition of the normal replication. No virus formation was detected in the cytoplasm. After 7 day treatment of EDU, the corneal epithelium is almost of normal structural appearance. Accordingly, the present results on the rabbit seem to correlate well with the reported therapeutic antiherpetic studies in the human cornea.", "contents": "[Clinical, light- and electron-microscopic investigations on the effect of 5-ethyl-2-deoxyuridine (EDU) in herpes simplex keratitis in rabbits (author's transl)]. Morphologic changes in rabbit cornea accompanying herpes simplex keratitis especially under the treatment of ethyldeoxyuridine (EDU) are summarized in regard to clinical aspects, light microscopy and electron microscopy. Untreated eyes show virus-dependent characteristic changes of the cell structure: peripheral migration of the cell chromatin, swelling of the nucleus, and disappearance of the nucleolus are persistent. In the nucleus as well as in the cytoplasm mature and immature virus particles are visible which demonstrate the normal virus-replication course. In the EDU treated cornea these particular changes are observed only in the primary stage. After prolonged treatment in the nucleus of the infected cells there are only immature virus particles with optically empty center. This can be evaluated as a sign of inhibition of the normal replication. No virus formation was detected in the cytoplasm. After 7 day treatment of EDU, the corneal epithelium is almost of normal structural appearance. Accordingly, the present results on the rabbit seem to correlate well with the reported therapeutic antiherpetic studies in the human cornea."}
{"id": "PMID:1082271", "title": "Ring-shaped alkali burns of the rabbit cornea. Epithelial and endothelial changes.", "content": "Rabbit corneas were burned by touching their surfaces with rings of Whatman No. 1 chromatographic paper soaked in 1N NaOH. The corneal epithelium and endothelium were studied by impregnating flat sections with McGovern's and Jabonero's silver stains. Immediate destruction as well as regeneration of both cellular layers is described.", "contents": "Ring-shaped alkali burns of the rabbit cornea. Epithelial and endothelial changes. Rabbit corneas were burned by touching their surfaces with rings of Whatman No. 1 chromatographic paper soaked in 1N NaOH. The corneal epithelium and endothelium were studied by impregnating flat sections with McGovern's and Jabonero's silver stains. Immediate destruction as well as regeneration of both cellular layers is described."}
{"id": "PMID:1082272", "title": "[Experimental study of the problem of retinal ischemia (author's transl)].", "content": "Survival and revival time of the retina in animal experiments are essentially dependent upon whether total ischemia occurs or not. Using the so-called barbiturate effect in the electroretinogram as a functional criterion, complete ischemia can be shown to take place in rabbit and cat at intraocular pressures of 100 mm Hg. At somewhat lower pressures, which already cause alterations of the ERG a remarked barbiturate effect can be observed, indicating the persistance of a certain amount of remaining blood flow. Possible mechanisms of the barbiturate effect in the retina are discussed.", "contents": "[Experimental study of the problem of retinal ischemia (author's transl)]. Survival and revival time of the retina in animal experiments are essentially dependent upon whether total ischemia occurs or not. Using the so-called barbiturate effect in the electroretinogram as a functional criterion, complete ischemia can be shown to take place in rabbit and cat at intraocular pressures of 100 mm Hg. At somewhat lower pressures, which already cause alterations of the ERG a remarked barbiturate effect can be observed, indicating the persistance of a certain amount of remaining blood flow. Possible mechanisms of the barbiturate effect in the retina are discussed."}
{"id": "PMID:1082273", "title": "[Experimental chorioretinitis in rabbits induced by homologous and heterologous photoreceptor outer segments (author's transl)].", "content": "Experiments with 73 GrCh rabbits showed, that both homologous and heterologous (bovine) photoreceptor outer segments (ROS) induce chorioretinitic diseases of the same kind. The findings refer to questions of localization and efficiency of homologous and heterologous (bovine) pathogenic ROS antigens.", "contents": "[Experimental chorioretinitis in rabbits induced by homologous and heterologous photoreceptor outer segments (author's transl)]. Experiments with 73 GrCh rabbits showed, that both homologous and heterologous (bovine) photoreceptor outer segments (ROS) induce chorioretinitic diseases of the same kind. The findings refer to questions of localization and efficiency of homologous and heterologous (bovine) pathogenic ROS antigens."}
{"id": "PMID:1082274", "title": "[Structure of the vitreous body over areas with lattice degeneration of the retina (author's transl)].", "content": "The clinical examination of the vitreous in the extreme fundus periphery is difficult; therefore only very little was known up to now about the relations of the posterior limiting membrane of the vitreous and the preretinal tract to retinal lattice degenerations arranged parallel to the ora serrata in several rows. There will be reports of new findings gained by depression biomicroscopy.", "contents": "[Structure of the vitreous body over areas with lattice degeneration of the retina (author's transl)]. The clinical examination of the vitreous in the extreme fundus periphery is difficult; therefore only very little was known up to now about the relations of the posterior limiting membrane of the vitreous and the preretinal tract to retinal lattice degenerations arranged parallel to the ora serrata in several rows. There will be reports of new findings gained by depression biomicroscopy."}
{"id": "PMID:1082275", "title": "[Problem of scattered light in the localized stimulation of the retina (author's transl)].", "content": "In attempting local retinal stimulation of an intact eye the generation of scattered light cannot be prevented, but it can be sufficiently reduced by a suitable selection of stimulus parameters, especially of stimulus luminance and facial field luminance, of stimulus size and exposition time. By investigating the most important sources of scattered light, e.g. inhomogenities in the dioptric media, reflections at refracting surfaces, diffraction at the pupil borders, and the distribution of scattered light in the retina it becomes possible to develop indications for the construction of a focal optical stimulator.", "contents": "[Problem of scattered light in the localized stimulation of the retina (author's transl)]. In attempting local retinal stimulation of an intact eye the generation of scattered light cannot be prevented, but it can be sufficiently reduced by a suitable selection of stimulus parameters, especially of stimulus luminance and facial field luminance, of stimulus size and exposition time. By investigating the most important sources of scattered light, e.g. inhomogenities in the dioptric media, reflections at refracting surfaces, diffraction at the pupil borders, and the distribution of scattered light in the retina it becomes possible to develop indications for the construction of a focal optical stimulator."}
{"id": "PMID:1082276", "title": "[Lesion of the retinal fatty acids following intravitreal injection of iron. An experimental study on the retinal lesions in cases of siderosis bulbi (author's transl)].", "content": "In this study the alterations of the pattern of the fatty acids of the retinal phosphatides after intravitreal injection of ferrous ammonium sulphate are treated. In vitro and in vivo a significant decrease of the highly unsaturated fatty acids of the C22 type occurs. This lesion spreads from the region of direct contact between iron and retina towards the periphery within 24 hs. The reliability of the experiment is ensured by the proof of analogous changes in human globes affected with siderosis. Finally, the production of lipid peroxides as a cause of the breakdown of the retinal function is discussed.", "contents": "[Lesion of the retinal fatty acids following intravitreal injection of iron. An experimental study on the retinal lesions in cases of siderosis bulbi (author's transl)]. In this study the alterations of the pattern of the fatty acids of the retinal phosphatides after intravitreal injection of ferrous ammonium sulphate are treated. In vitro and in vivo a significant decrease of the highly unsaturated fatty acids of the C22 type occurs. This lesion spreads from the region of direct contact between iron and retina towards the periphery within 24 hs. The reliability of the experiment is ensured by the proof of analogous changes in human globes affected with siderosis. Finally, the production of lipid peroxides as a cause of the breakdown of the retinal function is discussed."}
{"id": "PMID:1082278", "title": "An analysis of muscle weight variations in the hindlimbs of Rana esculenta.", "content": "Dry weights of 24 muscles of the left and of the right hindlimbs in 63 frogs (Rana esculenta) are determined. By means of factor analysis is calculated which part of the variance in a muscle's weight can be accounted for by common factors and which part by the characteristics of the muscle itself. The main results are: 1. Body size (factor 1) accounts for more than 90% of the variance in all the heavier muscles and more than 64% in the eight lighter muscles. 2. Since only one single common factor is found, no separate groups of highly correlated muscle weights are present in the frog's hindlimb. 3. A decrease in the number of fibers is coupled with an increase in the residual variance of a muscle. 4. Left and right identic muscles follow the same variation-pattern. Two models for muscle development are discussed: 1. A model in which the innervation of the muscle determines the number of fibers. 2. A model in which clumping play an important role.", "contents": "An analysis of muscle weight variations in the hindlimbs of Rana esculenta. Dry weights of 24 muscles of the left and of the right hindlimbs in 63 frogs (Rana esculenta) are determined. By means of factor analysis is calculated which part of the variance in a muscle's weight can be accounted for by common factors and which part by the characteristics of the muscle itself. The main results are: 1. Body size (factor 1) accounts for more than 90% of the variance in all the heavier muscles and more than 64% in the eight lighter muscles. 2. Since only one single common factor is found, no separate groups of highly correlated muscle weights are present in the frog's hindlimb. 3. A decrease in the number of fibers is coupled with an increase in the residual variance of a muscle. 4. Left and right identic muscles follow the same variation-pattern. Two models for muscle development are discussed: 1. A model in which the innervation of the muscle determines the number of fibers. 2. A model in which clumping play an important role."}
{"id": "PMID:1082280", "title": "Agammaglobulinemia in a horse with evidence of functional T lymphocytes.", "content": "Agammaglobulinemia was diagnosed in a 1-year-old Thoroughbred horse on the basis of the following observations: (1) absence of serum immunoglobulins M, A, and G(T); (2) small amounts of serum immunoglobulin G (16 mg/100 ml); (3) absence of specific antibody in the serum of the horse following immunization and challenge exposure to 2 antigens; (4) absence of plasma cells, primary follicles, and germinal centers in a lymph node removed after antigenic stimulation; (5) absence of \"natural\" serum antibodies to rabbit-erythrocytes which were easily detectable in age-matched control horse serums; and (6) increased susceptibility to infections. There was evidence of functional cell-mediated immunity which included a skin response to injected phytolectins, skin response to antigen challenge following sensitization, and in vitro proliferative response of lymph node cells to phytohemagglutinin. An intact cell-mediated immune response was also supported by the observation that the horse lived to 17 months of age without antibody production, whereas horses with an absence of both antibody production and cell-mediated immunity (combined immunodeficiency) die by 4 months of age without immunologic intervention. The known features of agammaglobulinemia in this horse are similar to those in sex-linked agammaglobulinemia in persons and are unique among the immunodeficiences described in other animals.", "contents": "Agammaglobulinemia in a horse with evidence of functional T lymphocytes. Agammaglobulinemia was diagnosed in a 1-year-old Thoroughbred horse on the basis of the following observations: (1) absence of serum immunoglobulins M, A, and G(T); (2) small amounts of serum immunoglobulin G (16 mg/100 ml); (3) absence of specific antibody in the serum of the horse following immunization and challenge exposure to 2 antigens; (4) absence of plasma cells, primary follicles, and germinal centers in a lymph node removed after antigenic stimulation; (5) absence of \"natural\" serum antibodies to rabbit-erythrocytes which were easily detectable in age-matched control horse serums; and (6) increased susceptibility to infections. There was evidence of functional cell-mediated immunity which included a skin response to injected phytolectins, skin response to antigen challenge following sensitization, and in vitro proliferative response of lymph node cells to phytohemagglutinin. An intact cell-mediated immune response was also supported by the observation that the horse lived to 17 months of age without antibody production, whereas horses with an absence of both antibody production and cell-mediated immunity (combined immunodeficiency) die by 4 months of age without immunologic intervention. The known features of agammaglobulinemia in this horse are similar to those in sex-linked agammaglobulinemia in persons and are unique among the immunodeficiences described in other animals."}
{"id": "PMID:1082281", "title": "Distributions of immunoglobulin-containing cells in alimentary tract, spleen, and mesenteric lymph node of the pig demonstrated by peroxidase-conjugated antiserums to porcine immunoglobulins G, A, and M.", "content": "Peroxidase-conjugated antibody to porcine immunoglobulins A, G, and M were used in a light microscope study of the distribution of immunoglobulins and immunoglobulin-containing cells in alimentary tract, spleen, and mesenteric lymph node of the pig. Largest populations of plasma cells staining for each of the 3 immunoglobulin classes were demonstrated in the lamina propria of the intestinal tract. Immunoglobulin A cells were most numerous, and immunoglobulin A and M cells each outnumbered immunoglobulin G cells which were, however, present in considerable numbers. Both immunoglobulins A and M were demonstrated in intestinal crypt epithelial cells, indicating they have similar intracellular secretion pathways. Porcine spleen and mesenteric lymph node seem to have a minor role in immunoglobulin synthesis.", "contents": "Distributions of immunoglobulin-containing cells in alimentary tract, spleen, and mesenteric lymph node of the pig demonstrated by peroxidase-conjugated antiserums to porcine immunoglobulins G, A, and M. Peroxidase-conjugated antibody to porcine immunoglobulins A, G, and M were used in a light microscope study of the distribution of immunoglobulins and immunoglobulin-containing cells in alimentary tract, spleen, and mesenteric lymph node of the pig. Largest populations of plasma cells staining for each of the 3 immunoglobulin classes were demonstrated in the lamina propria of the intestinal tract. Immunoglobulin A cells were most numerous, and immunoglobulin A and M cells each outnumbered immunoglobulin G cells which were, however, present in considerable numbers. Both immunoglobulins A and M were demonstrated in intestinal crypt epithelial cells, indicating they have similar intracellular secretion pathways. Porcine spleen and mesenteric lymph node seem to have a minor role in immunoglobulin synthesis."}
{"id": "PMID:1082282", "title": "A new deficient variant of alpha1-antitrypsin (MDUARTE). Inability to detect the heterozygous state by antitrypsin phenotyping.", "content": "A new molecular variant of alpha1-antitrypsin was discovered in the family of a woman with severe antitrypsin deficiency and bullous emphysema. The variant resembles the Z variant in most respects in that it results in severe antitrypsin deficiency with the homozygous state and intermediate deficiency with the heterozygous state, and is associated with diastase-resistant, periodic acid-Schiff-positive globules in the liver cells. It differs from the usual Z variant, however, by having normal mobility on acid-starch electrophoresis so that the heterozygous state with the normal M form cannot be distinguished by phenotyping procedures on either acid-starch or alkaline-agarose electrophoresis. The variant has been labeled MDUARTE. A review of phenotype patterns in all patients previously classified as having a homozygous ZZ phenotype reveals extra, fast-moving bands on acid-starch suggestive of an MDUARTEZ heterozygous state in 7.9 per cent of such cases. When intermediate antitrypsin deficiency occurs in the presence of a normal phenotype pattern, one must consider that the patient has inherited either a null gene for antitrypsin synthesis or an MDUARTE variant.", "contents": "A new deficient variant of alpha1-antitrypsin (MDUARTE). Inability to detect the heterozygous state by antitrypsin phenotyping. A new molecular variant of alpha1-antitrypsin was discovered in the family of a woman with severe antitrypsin deficiency and bullous emphysema. The variant resembles the Z variant in most respects in that it results in severe antitrypsin deficiency with the homozygous state and intermediate deficiency with the heterozygous state, and is associated with diastase-resistant, periodic acid-Schiff-positive globules in the liver cells. It differs from the usual Z variant, however, by having normal mobility on acid-starch electrophoresis so that the heterozygous state with the normal M form cannot be distinguished by phenotyping procedures on either acid-starch or alkaline-agarose electrophoresis. The variant has been labeled MDUARTE. A review of phenotype patterns in all patients previously classified as having a homozygous ZZ phenotype reveals extra, fast-moving bands on acid-starch suggestive of an MDUARTEZ heterozygous state in 7.9 per cent of such cases. When intermediate antitrypsin deficiency occurs in the presence of a normal phenotype pattern, one must consider that the patient has inherited either a null gene for antitrypsin synthesis or an MDUARTE variant."}
{"id": "PMID:1082283", "title": "Classification of bronchial lymphocytes from nonsmokers and smokers.", "content": "The population of lymphocytes obtained by endobronchial lavage is significantly different from the distribution of lymphocytes in the peripheral blood. Both B and T lymphocytes are found in fluids lavaged from the normal human lung. Of the lymphocytes that can be classified, the T cell population is larger than the B cell population in both nonsmokers and cigarette smokers, whereas a further increment in T cells is noted in smokers. A third population of unclassified lymphocytes, the \"null\" cells, also comprise a large portion of the lymphocyte population in both smokers and nonsmokers. Their significance has yet to be determined.", "contents": "Classification of bronchial lymphocytes from nonsmokers and smokers. The population of lymphocytes obtained by endobronchial lavage is significantly different from the distribution of lymphocytes in the peripheral blood. Both B and T lymphocytes are found in fluids lavaged from the normal human lung. Of the lymphocytes that can be classified, the T cell population is larger than the B cell population in both nonsmokers and cigarette smokers, whereas a further increment in T cells is noted in smokers. A third population of unclassified lymphocytes, the \"null\" cells, also comprise a large portion of the lymphocyte population in both smokers and nonsmokers. Their significance has yet to be determined."}
{"id": "PMID:1082284", "title": "Aortocoronary bypass in Jehovah's Witnesses: review of 46 patients.", "content": "The hemodilution technique for cardiopulmonary bypass using blood substitutes for priming has permitted open heart operations in Jehovah's Witnesses who refuse to accept blood, and has reduced the need for massive blood transfusion in certain procedures including aortocoronary bypass. A series of 46 Jehovah's Witness patients underwent aortocoronary bypass procedures. Of these, two patients died, representing a mortality of 4.3 per cent. Neither patient's death was related to lack of blood transfusions. The hospital stay and recovery time of all the other patients was not affected by failure to transfuse blood. The excellent short- and long-term results of this particular group paralleled those observed in our larger series of over 2700 other patients who have undergone coronary bypass surgery since 1969. Among these patients not of the Jehovah's Witness religion, blood transfusion was not necessary in about 30 per cent, while the remainder averaged less than two units per patient. Our results with Jehovah's Witness patients encourage our policy of avoiding blood transfusions whenever possible in all operations. Further justification for our conservative attitude is provided by the current shortage of blood in relation to a projected continuous increase of aortocoronary bypass procedures in the future.", "contents": "Aortocoronary bypass in Jehovah's Witnesses: review of 46 patients. The hemodilution technique for cardiopulmonary bypass using blood substitutes for priming has permitted open heart operations in Jehovah's Witnesses who refuse to accept blood, and has reduced the need for massive blood transfusion in certain procedures including aortocoronary bypass. A series of 46 Jehovah's Witness patients underwent aortocoronary bypass procedures. Of these, two patients died, representing a mortality of 4.3 per cent. Neither patient's death was related to lack of blood transfusions. The hospital stay and recovery time of all the other patients was not affected by failure to transfuse blood. The excellent short- and long-term results of this particular group paralleled those observed in our larger series of over 2700 other patients who have undergone coronary bypass surgery since 1969. Among these patients not of the Jehovah's Witness religion, blood transfusion was not necessary in about 30 per cent, while the remainder averaged less than two units per patient. Our results with Jehovah's Witness patients encourage our policy of avoiding blood transfusions whenever possible in all operations. Further justification for our conservative attitude is provided by the current shortage of blood in relation to a projected continuous increase of aortocoronary bypass procedures in the future."}
{"id": "PMID:1082285", "title": "Diagnosis and management of occult gastrointestinal bleeding: visualization of the small bowel lumen by fiberoptic colonoscope.", "content": "In 95 per cent of patients, the source of gastrointestinal bleeding can be determined by the usual roentgenographic and endoscopic methods. In the remaining five per cent of patients, the source of bleeding is obscure and special diagnostic procedures will be necessary. Among the special procedures that have been reported are the fluorescein string test, stepwise aspiration of intestinal contents, detection of radioactive red blood cells by stepwise aspiration, Geiger counter or scanning, and selective visceral angiography. An additional procedure, reported here, combines exploratory celiotomy with total endoscopic examination of the bowel.", "contents": "Diagnosis and management of occult gastrointestinal bleeding: visualization of the small bowel lumen by fiberoptic colonoscope. In 95 per cent of patients, the source of gastrointestinal bleeding can be determined by the usual roentgenographic and endoscopic methods. In the remaining five per cent of patients, the source of bleeding is obscure and special diagnostic procedures will be necessary. Among the special procedures that have been reported are the fluorescein string test, stepwise aspiration of intestinal contents, detection of radioactive red blood cells by stepwise aspiration, Geiger counter or scanning, and selective visceral angiography. An additional procedure, reported here, combines exploratory celiotomy with total endoscopic examination of the bowel."}
{"id": "PMID:1082286", "title": "Fleck (Mouchet\u00e9e) dystrophy of the cornea.", "content": "Five families, four Caucasian and one Negro (14 patients) with fleck (speckled or Mouchet\u00e9e) dystrophy of the cornea are presented. In each, the typical presentation of fine scattered fleck-like dystrophic lesions was found throughout all layers of the central and peripheral corneal stroma but not affecting the epithelium, Bowman's membrane, Descemet's membrane or the endothelium. Clear stroma was noted between each lesion. Visual acuity in all patients was normal or only slightly affected. Except for an occasional patient with minor photophobia, almost all patients found affected with this dystrophy were asymptomatic in regard to their corneal condition. There was no decrease in corneal sensitivity noted in any patient. All families displayed evidence of an autosomal dominant hereditary pattern and progression of the dystrophy is slow and benign in character.  With the exception of one family with atopic disease, no systemic organic illness was noted by histroy in all of the families. Laboratory screening of 2 of the 5 families showed no abnormalities of any systemic metabolic disorders. The characteristic clinical picture and favorable prognosis of this condition enables one to easily differentiate this condition from other known parenchymatous corneal dystrophies. The incidence of this condition is probably much more common than the reported cases in the literature might indicate.", "contents": "Fleck (Mouchet\u00e9e) dystrophy of the cornea. Five families, four Caucasian and one Negro (14 patients) with fleck (speckled or Mouchet\u00e9e) dystrophy of the cornea are presented. In each, the typical presentation of fine scattered fleck-like dystrophic lesions was found throughout all layers of the central and peripheral corneal stroma but not affecting the epithelium, Bowman's membrane, Descemet's membrane or the endothelium. Clear stroma was noted between each lesion. Visual acuity in all patients was normal or only slightly affected. Except for an occasional patient with minor photophobia, almost all patients found affected with this dystrophy were asymptomatic in regard to their corneal condition. There was no decrease in corneal sensitivity noted in any patient. All families displayed evidence of an autosomal dominant hereditary pattern and progression of the dystrophy is slow and benign in character.  With the exception of one family with atopic disease, no systemic organic illness was noted by histroy in all of the families. Laboratory screening of 2 of the 5 families showed no abnormalities of any systemic metabolic disorders. The characteristic clinical picture and favorable prognosis of this condition enables one to easily differentiate this condition from other known parenchymatous corneal dystrophies. The incidence of this condition is probably much more common than the reported cases in the literature might indicate."}
{"id": "PMID:1082287", "title": "Diagnostic and therapeutic vitrectomy for endophthalmitis.", "content": "The introduction of a compact portable vitreous suction cutter enables the aspiration and resection of infected ocular tissues for diagnostic and therapeutic purposes. Four cases of postoperative bacterial endophthalmitis are presented in which mechanical anterior vitrectomy was performed in addition to conventional antimicrobial therapy. Three of these eyes were saved and 2 retained the possibility of useful vision. A rationale for the aggressive managements of endophthalmitis is presented.", "contents": "Diagnostic and therapeutic vitrectomy for endophthalmitis. The introduction of a compact portable vitreous suction cutter enables the aspiration and resection of infected ocular tissues for diagnostic and therapeutic purposes. Four cases of postoperative bacterial endophthalmitis are presented in which mechanical anterior vitrectomy was performed in addition to conventional antimicrobial therapy. Three of these eyes were saved and 2 retained the possibility of useful vision. A rationale for the aggressive managements of endophthalmitis is presented."}
{"id": "PMID:1082288", "title": "Haemophilus influenzae as a cause of salpingitis.", "content": "A case is reported of acute unilateral salpingitis, the cause of which was Haemophilus influenzae cultured from a pus sample taken from the infected Fallopian tube during laparotomy. The significance of the findings is discussed with reference to the literature.", "contents": "Haemophilus influenzae as a cause of salpingitis. A case is reported of acute unilateral salpingitis, the cause of which was Haemophilus influenzae cultured from a pus sample taken from the infected Fallopian tube during laparotomy. The significance of the findings is discussed with reference to the literature."}
{"id": "PMID:1082292", "title": "Microdilution technique for antimicrobial susceptibility testing of Haemophilus influenzae.", "content": "A microdilution method incorporating the use of color-defined growth end points was compared with a conventional broth tube dilution procedure for susceptibility testing of Haemophilus influenzae with ampicillin and chloramphenicol. The microdilution method allowed rapid performance of dilution susceptibility tests with easily defined end points.", "contents": "Microdilution technique for antimicrobial susceptibility testing of Haemophilus influenzae. A microdilution method incorporating the use of color-defined growth end points was compared with a conventional broth tube dilution procedure for susceptibility testing of Haemophilus influenzae with ampicillin and chloramphenicol. The microdilution method allowed rapid performance of dilution susceptibility tests with easily defined end points."}
{"id": "PMID:1082289", "title": "The relationship between the T-helper cell and the T-cell involved in the delayed hypersensitivity.", "content": "The common cell type involved in the humoral and cellular responses is the T-cell. Functionally the T-cell, active in the humoral response, is known as Thelper (Th), the T-cell active in the cellular response as Tcellular (Tc). The present experiments are used to study the relationship between Tc and Th. IP immunization, with suboptimal doses of antigen, did not result in antibody formation but induced both a DH (equals Tc) and a Th population. Th and Tc activity changed in a similar way with increasing doses of antigen. Spleen cells taken from mice, which were immunized under conditions which only invoke cellular activity, were able to function even in small numbers as Th in transfer experiments. Educated thymus cells and cells obtained from cortisone or CY treated donor mice had an increased Th and Tc activity when transferred into irradiated recipients. Education in the presence of a high antigen dose reduced both activities below control levels. Cross reactivity between HRBC and SRBC in the hormoral response is known to be at the Th level. It was demonstrated that this cross reactivity also exists at the Tc level. Induction of DH with one antigen acted as pre-immunization, for the humoral response, on the cross reactive antigen too. These results suggest a correlation between the T-cells involved in DH and the Th for antibody formation.", "contents": "The relationship between the T-helper cell and the T-cell involved in the delayed hypersensitivity. The common cell type involved in the humoral and cellular responses is the T-cell. Functionally the T-cell, active in the humoral response, is known as Thelper (Th), the T-cell active in the cellular response as Tcellular (Tc). The present experiments are used to study the relationship between Tc and Th. IP immunization, with suboptimal doses of antigen, did not result in antibody formation but induced both a DH (equals Tc) and a Th population. Th and Tc activity changed in a similar way with increasing doses of antigen. Spleen cells taken from mice, which were immunized under conditions which only invoke cellular activity, were able to function even in small numbers as Th in transfer experiments. Educated thymus cells and cells obtained from cortisone or CY treated donor mice had an increased Th and Tc activity when transferred into irradiated recipients. Education in the presence of a high antigen dose reduced both activities below control levels. Cross reactivity between HRBC and SRBC in the hormoral response is known to be at the Th level. It was demonstrated that this cross reactivity also exists at the Tc level. Induction of DH with one antigen acted as pre-immunization, for the humoral response, on the cross reactive antigen too. These results suggest a correlation between the T-cells involved in DH and the Th for antibody formation."}
{"id": "PMID:1082293", "title": "Influences of media and inocula on the in vitro susceptibility of Haemophilus influenzae to co-trimoxazole, ampicillin, penicillin, and chloramphenicol.", "content": "The effects of inocula and media on the activities of ampicillin, penicillin, chloramphenicol and co-trimoxazole against Haemophilus influenzae were examined in vitro. Two inocula and four media were tested by the disk diffusion, broth dilution, and agar dilution methods. Chloramphenicol activity versus H. influenzae was least affected by changes in inocula and media, whereas co-trimoxazole was most susceptible to these effects. Filde's and Levinthal's agar dilution tests were most satisfactory for ampicillin. Penicillin was less active on Levinthal's than on Filde's agar. Both ampicillin and penicillin were less active when tested against the higher inoculum. Co-trimoxazole was most active (<1% H. influenzae was resistant) when tested at an inoculum of 10(6) colony-forming units/ml on diagnostic susceptibility test agar with 5% lysed horse blood added. The majority of H. influenzae appeared resistant to co-trimoxazole with increases in the test inocula and/or when tested on brain heart infusion with Filde's, Levinthal's or \"low-thymidine\" Mueller-Hinton medium.", "contents": "Influences of media and inocula on the in vitro susceptibility of Haemophilus influenzae to co-trimoxazole, ampicillin, penicillin, and chloramphenicol. The effects of inocula and media on the activities of ampicillin, penicillin, chloramphenicol and co-trimoxazole against Haemophilus influenzae were examined in vitro. Two inocula and four media were tested by the disk diffusion, broth dilution, and agar dilution methods. Chloramphenicol activity versus H. influenzae was least affected by changes in inocula and media, whereas co-trimoxazole was most susceptible to these effects. Filde's and Levinthal's agar dilution tests were most satisfactory for ampicillin. Penicillin was less active on Levinthal's than on Filde's agar. Both ampicillin and penicillin were less active when tested against the higher inoculum. Co-trimoxazole was most active (<1% H. influenzae was resistant) when tested at an inoculum of 10(6) colony-forming units/ml on diagnostic susceptibility test agar with 5% lysed horse blood added. The majority of H. influenzae appeared resistant to co-trimoxazole with increases in the test inocula and/or when tested on brain heart infusion with Filde's, Levinthal's or \"low-thymidine\" Mueller-Hinton medium."}
{"id": "PMID:1082290", "title": "Effect of stimulation with bacterial lipopolysaccharide on the surface-charge of mouse B-lymphocytes.", "content": "Nude spleen (B) cells were cultivated in vitro in a serum-free medium, without or with lipopolysaccharide (LPS) and their surface-charge was measured by cell electrophoresis. In the absence of LPS, surface-charge did not vary significantly with time, whereas, in the presence of LPS a biphasic modification was observed. Thus, after 4 hours of culture with LPS, surface-charge was diminished by 14%. Subsequently, when DNA synthesis and blast formation were activated, it increased by up to 19-23% over control value, whether LPS was present continuously in the medium or had been eliminated by washing after 4 hours of incubation.", "contents": "Effect of stimulation with bacterial lipopolysaccharide on the surface-charge of mouse B-lymphocytes. Nude spleen (B) cells were cultivated in vitro in a serum-free medium, without or with lipopolysaccharide (LPS) and their surface-charge was measured by cell electrophoresis. In the absence of LPS, surface-charge did not vary significantly with time, whereas, in the presence of LPS a biphasic modification was observed. Thus, after 4 hours of culture with LPS, surface-charge was diminished by 14%. Subsequently, when DNA synthesis and blast formation were activated, it increased by up to 19-23% over control value, whether LPS was present continuously in the medium or had been eliminated by washing after 4 hours of incubation."}
{"id": "PMID:1082291", "title": "Migration of intramediastinally injected thymocytes in draining nodes.", "content": "The present study investigated the migration, in the lymph node, of cells entering the organ via the subcapsular sinus. Thymocytes of rats were labelled with 3H-thymidine and injected into the mediastinal cavity of syngeneic recipients. These animals were sacrificed at various intervals after injection. Five minutes after injection, labelled cells were found in the subcapsular sinus, and even in the peripheral cortex, of the draining mediastinal lymph nodes. The overall of the radioautographic observations reveals a migration of cells from the peripheral cortex towards the deep cortex, and into the medullary cords. The observations indicate that such migration can occur in less than 12 hours. Nevertheless, six hours after the injection, some labelled cells had escaped already the draining nodes and migrated into the other lymphoid organs. It is a fact that some of our results suggest that cells may pass directly from the subcapsular into the medullary sinuses without previously migrating into the nodal parenchyma. As to folliculo-nodules and pseudo-follicles, they exhibited relatively few labelled cells. Moreover, some of our findings indicated that cell migration was much slower in the pseudo-follicles than in the extrafollicular zone.", "contents": "Migration of intramediastinally injected thymocytes in draining nodes. The present study investigated the migration, in the lymph node, of cells entering the organ via the subcapsular sinus. Thymocytes of rats were labelled with 3H-thymidine and injected into the mediastinal cavity of syngeneic recipients. These animals were sacrificed at various intervals after injection. Five minutes after injection, labelled cells were found in the subcapsular sinus, and even in the peripheral cortex, of the draining mediastinal lymph nodes. The overall of the radioautographic observations reveals a migration of cells from the peripheral cortex towards the deep cortex, and into the medullary cords. The observations indicate that such migration can occur in less than 12 hours. Nevertheless, six hours after the injection, some labelled cells had escaped already the draining nodes and migrated into the other lymphoid organs. It is a fact that some of our results suggest that cells may pass directly from the subcapsular into the medullary sinuses without previously migrating into the nodal parenchyma. As to folliculo-nodules and pseudo-follicles, they exhibited relatively few labelled cells. Moreover, some of our findings indicated that cell migration was much slower in the pseudo-follicles than in the extrafollicular zone."}
{"id": "PMID:1082294", "title": "The degradation of paracetamol (4-hydroxyacetanilide) and other substituted acetanilides by a Penicillium species.", "content": "A mould which was isolated from a solution of paracetamol was identified as a Penicillium species and was found to possess the ability to utilise a series of substituted acetanilides, including paracetamol (4-hydroxyacetanilide), phenacetin (4-ethoxyacetanilide) and metacetamol (3-hydroxyacetanilide) as sole carbon sources for growth. Studies with washed-cell suspensions indicated that growth of the Penicillium isolate in the presence of paracetamol induced the respective enzyme systems for the degradation of this compound. Manometric studies measuring oxygen uptake rates, indicated that the mould was capable of degrading paracetamol to acetate and 4-aminophenol. Acetate was further metabolised whilst 4-aminophenol accumulated in the growth medium and was subsequently i-entified by UV spectroscopy and thin-layer chromatography. Similar experiments with phenacetin indicated metabolism by the mould to acetate and 4-ethoxyaniline which was isolated and identified by subsequent analysis of the growth medium. However, unlike 4-aminophenol and 4-ethoxyaniline, the degradation product (3-aminophenol) from metacetamol metabolism was further degraded by the mould.", "contents": "The degradation of paracetamol (4-hydroxyacetanilide) and other substituted acetanilides by a Penicillium species. A mould which was isolated from a solution of paracetamol was identified as a Penicillium species and was found to possess the ability to utilise a series of substituted acetanilides, including paracetamol (4-hydroxyacetanilide), phenacetin (4-ethoxyacetanilide) and metacetamol (3-hydroxyacetanilide) as sole carbon sources for growth. Studies with washed-cell suspensions indicated that growth of the Penicillium isolate in the presence of paracetamol induced the respective enzyme systems for the degradation of this compound. Manometric studies measuring oxygen uptake rates, indicated that the mould was capable of degrading paracetamol to acetate and 4-aminophenol. Acetate was further metabolised whilst 4-aminophenol accumulated in the growth medium and was subsequently i-entified by UV spectroscopy and thin-layer chromatography. Similar experiments with phenacetin indicated metabolism by the mould to acetate and 4-ethoxyaniline which was isolated and identified by subsequent analysis of the growth medium. However, unlike 4-aminophenol and 4-ethoxyaniline, the degradation product (3-aminophenol) from metacetamol metabolism was further degraded by the mould."}
{"id": "PMID:1082295", "title": "RNA synthesis during zoospore germination in the water mold Allomyces arbuscula.", "content": "The major classes of RNA (rRNA, mRNA, tRNA) start to be synthesized simultaneously in the zoospore of Allomyces soon after the onset of germination. Especially during cyst formation and germ tube emergence, the synthesis of mRNA and tRNA is prominent whereas that of rRNA proceeds slowly. The transcription products of these early stages are needed for the subsequent initiation of hypha formation.", "contents": "RNA synthesis during zoospore germination in the water mold Allomyces arbuscula. The major classes of RNA (rRNA, mRNA, tRNA) start to be synthesized simultaneously in the zoospore of Allomyces soon after the onset of germination. Especially during cyst formation and germ tube emergence, the synthesis of mRNA and tRNA is prominent whereas that of rRNA proceeds slowly. The transcription products of these early stages are needed for the subsequent initiation of hypha formation."}
{"id": "PMID:1082296", "title": "Improvements of the membrane filter method for DNA:rRNA hybridization.", "content": "We describe and recommend the following improvements of DNA:rRNA membrane filter hybridization methods. One of our aims was to avoid DNA release from filter discs during hybridization. 1. Our hybridization conditions are 2 SSC in aq. dest., with 20% formamide, 50 C, overnight for 16 hr. 2. Duplexing is over in 8-10 hr. 3. Formamide has to be very pure (O.D. less than or equal to 0.2/cm light path at 270 nm). 4. RNAase treatment: 250 mug/5 ml 2 SSC/filter at 37 C for 1 hr. 5. Our conditions for stepwise thermal denaturation are: 5 degrees C steps from 50 C to 90 C in 1.5 SSC in 20% formamide. 6. Single-stranded DNA, fixed on membrane filters, and stored in vacuo at 4C can be used reliably for hybridization for up to 20 months. 7. Concentrated DNA in 0.1 SSC, quick-frozen at -50 C and stored at -90 C for up to 2 years can be used for hybridization without much change. 8. A CsCl gradient purification step yields much purer DNA, but increases the release of DNA from filters by about 20%. Filters with 20% more DAN is a compensation. 9. rRNA can be stored for 20 months in SSC or 2 SSC at -12 C without changing the hybridization results.", "contents": "Improvements of the membrane filter method for DNA:rRNA hybridization. We describe and recommend the following improvements of DNA:rRNA membrane filter hybridization methods. One of our aims was to avoid DNA release from filter discs during hybridization. 1. Our hybridization conditions are 2 SSC in aq. dest., with 20% formamide, 50 C, overnight for 16 hr. 2. Duplexing is over in 8-10 hr. 3. Formamide has to be very pure (O.D. less than or equal to 0.2/cm light path at 270 nm). 4. RNAase treatment: 250 mug/5 ml 2 SSC/filter at 37 C for 1 hr. 5. Our conditions for stepwise thermal denaturation are: 5 degrees C steps from 50 C to 90 C in 1.5 SSC in 20% formamide. 6. Single-stranded DNA, fixed on membrane filters, and stored in vacuo at 4C can be used reliably for hybridization for up to 20 months. 7. Concentrated DNA in 0.1 SSC, quick-frozen at -50 C and stored at -90 C for up to 2 years can be used for hybridization without much change. 8. A CsCl gradient purification step yields much purer DNA, but increases the release of DNA from filters by about 20%. Filters with 20% more DAN is a compensation. 9. rRNA can be stored for 20 months in SSC or 2 SSC at -12 C without changing the hybridization results."}
{"id": "PMID:1082297", "title": "A genetic study of tolerance and resistance to colicin A in Citrobacter freundii.", "content": "Colicin A-insensitive mutants of Citrobacter freundii were isolated and grouped into six phenotypic classes characterized by sensitivity, insensitivity or partial insensitivity to the bacteriocins S6, DF 13 and colicin A, and sensitivity or insensitivity to deoxycholate (DOC) and ampicillin. Mapping by the gradient-of-transmission method revealed the chromosomal regions in which the responsible genes are situated. Res-3 mapped near pur between pur and thr; Tol-5 mapped between aro and ilv and Tol-4 between gal and pur; Tol-1, Tol-2 and Tol-3 are situated close to gal. All the mutations that mapped near gal rendered the bacteria more sensitive to DOC and ampicillin. Complementation analysis with E. coli plasmids showed that the three phenotypic groups that map near gal were complemented by E. coli plasmids and fall into three complementation groups. Two of these are quivalent with the tol A and tol B genes in E. coli.", "contents": "A genetic study of tolerance and resistance to colicin A in Citrobacter freundii. Colicin A-insensitive mutants of Citrobacter freundii were isolated and grouped into six phenotypic classes characterized by sensitivity, insensitivity or partial insensitivity to the bacteriocins S6, DF 13 and colicin A, and sensitivity or insensitivity to deoxycholate (DOC) and ampicillin. Mapping by the gradient-of-transmission method revealed the chromosomal regions in which the responsible genes are situated. Res-3 mapped near pur between pur and thr; Tol-5 mapped between aro and ilv and Tol-4 between gal and pur; Tol-1, Tol-2 and Tol-3 are situated close to gal. All the mutations that mapped near gal rendered the bacteria more sensitive to DOC and ampicillin. Complementation analysis with E. coli plasmids showed that the three phenotypic groups that map near gal were complemented by E. coli plasmids and fall into three complementation groups. Two of these are quivalent with the tol A and tol B genes in E. coli."}
{"id": "PMID:1082298", "title": "Halteromyces, a new genus in the Mucorales.", "content": "Halteromyces radiatus is described as a new genus and species in the order Mucorales. The genus is referred to the family Mucoracease and has close affinities to the genus Absidia. The fungus grows readily in pure culture. The asexual structures are described; no sexual structures have been found despite extensive contrasts with members of the genus Absidia and Gongronella.", "contents": "Halteromyces, a new genus in the Mucorales. Halteromyces radiatus is described as a new genus and species in the order Mucorales. The genus is referred to the family Mucoracease and has close affinities to the genus Absidia. The fungus grows readily in pure culture. The asexual structures are described; no sexual structures have been found despite extensive contrasts with members of the genus Absidia and Gongronella."}
{"id": "PMID:1082299", "title": "Additional notes on species of Aspergillus, Eurotium and Emericella from Egyptian desert soil.", "content": "Aspergillus floriformis, A. pseudodeflectus, Eurotium xerophilum (st. con. A. xerophilus) and Emericella purpurea (st. con. A. purpureus) are described and illustrated as new species. In addition the morphology of strains identified as Aspergillus melleus, A. caespitosus and A. versicolor is discussed.", "contents": "Additional notes on species of Aspergillus, Eurotium and Emericella from Egyptian desert soil. Aspergillus floriformis, A. pseudodeflectus, Eurotium xerophilum (st. con. A. xerophilus) and Emericella purpurea (st. con. A. purpureus) are described and illustrated as new species. In addition the morphology of strains identified as Aspergillus melleus, A. caespitosus and A. versicolor is discussed."}
{"id": "PMID:1082300", "title": "On the subdivision of the genus Ceratocystis.", "content": "The desirability is discussed of a subdivison of the genus Ceratocystis into a group characterized by the presence of a phialidic conidial state, and a group in which the conidia are usually produced exogenously. Corroborative evidence for this arrangement is found in the carbohydrate constitution of the cells: in all examined species of the former group (Ceratocystis s. str.) rhamnose and cellulose are absent, whereas in the latter both components are present (Ophiostoma H. et P. Sydow).", "contents": "On the subdivision of the genus Ceratocystis. The desirability is discussed of a subdivison of the genus Ceratocystis into a group characterized by the presence of a phialidic conidial state, and a group in which the conidia are usually produced exogenously. Corroborative evidence for this arrangement is found in the carbohydrate constitution of the cells: in all examined species of the former group (Ceratocystis s. str.) rhamnose and cellulose are absent, whereas in the latter both components are present (Ophiostoma H. et P. Sydow)."}
{"id": "PMID:1082301", "title": "Trichosporon terrestre sp. nov.", "content": "A single strain representative of an undescribed Trichosporon species, was recovered from soil. The cell wall of the strain corresponds in structure to cell walls of the ascomycetous yeasts. On the ground of X-ray inactivation data the strain is assumed to be diploid. A description of the new species is given.", "contents": "Trichosporon terrestre sp. nov. A single strain representative of an undescribed Trichosporon species, was recovered from soil. The cell wall of the strain corresponds in structure to cell walls of the ascomycetous yeasts. On the ground of X-ray inactivation data the strain is assumed to be diploid. A description of the new species is given."}
{"id": "PMID:1082310", "title": "Gastrointestinal hemorrhage from adhesion-related mesenteric varices.", "content": "As a result of this retrospective analysis of hemorrhage from a porta-systemic venous shunt occurring within the small intestine, we believe that the early diagnosis of the syndrome is strongly suggested by the presence of varices in unusual locations demonstrated by the venous phase of mesenteric arteriography. In all patients portal hypertension was present, and in all the affected bowel was adherent to postoperative adhesions on old suture lines. The syndrome was treated variously with lysis of adhesions, bowel resection, or portal-systemic shunt. Those patients with excellent hepatic reserve survived and had no further gastrointestinal bleeding.", "contents": "Gastrointestinal hemorrhage from adhesion-related mesenteric varices. As a result of this retrospective analysis of hemorrhage from a porta-systemic venous shunt occurring within the small intestine, we believe that the early diagnosis of the syndrome is strongly suggested by the presence of varices in unusual locations demonstrated by the venous phase of mesenteric arteriography. In all patients portal hypertension was present, and in all the affected bowel was adherent to postoperative adhesions on old suture lines. The syndrome was treated variously with lysis of adhesions, bowel resection, or portal-systemic shunt. Those patients with excellent hepatic reserve survived and had no further gastrointestinal bleeding."}
{"id": "PMID:1082311", "title": "Mesocaval \"H\" graft using antogenous vein graft.", "content": "A group of 22 patients with bleeding esophageal varices were treated by creating mesocaval \"H\" shunts using autogenous veins as the \"H\" graft. The right external iliac vein was selected as the graft because of its appropriate size in diameter and length, its strength and elasticity. Details in operative technique are outlined. The age of our patients ranged from 24 to 75 years (average age 49), 19 patients had preoperative endoscopy, 10 patients received intraarterial pitressin drip to control hemorrhage, and surgery was done within 10 days of admission in 18 patients. There were two postoperative deaths (both patients Childs Class C), and very few postoperative problems. Right leg edema was a temporary and minimal problem. Encephalopathy was a major problem in only three patients, all of whom had poor hepatic reserve preoperatively. There has been no recurrent variceal bleeding in any of these patients. Our previous experimental data in dogs indicated that the long term patency of these shunts could be assured.", "contents": "Mesocaval \"H\" graft using antogenous vein graft. A group of 22 patients with bleeding esophageal varices were treated by creating mesocaval \"H\" shunts using autogenous veins as the \"H\" graft. The right external iliac vein was selected as the graft because of its appropriate size in diameter and length, its strength and elasticity. Details in operative technique are outlined. The age of our patients ranged from 24 to 75 years (average age 49), 19 patients had preoperative endoscopy, 10 patients received intraarterial pitressin drip to control hemorrhage, and surgery was done within 10 days of admission in 18 patients. There were two postoperative deaths (both patients Childs Class C), and very few postoperative problems. Right leg edema was a temporary and minimal problem. Encephalopathy was a major problem in only three patients, all of whom had poor hepatic reserve preoperatively. There has been no recurrent variceal bleeding in any of these patients. Our previous experimental data in dogs indicated that the long term patency of these shunts could be assured."}
{"id": "PMID:1082312", "title": "Peripheral vascular anastomotic aneurysms: a fifteen-year experience.", "content": "A 15-year experience with anastomotic aneurysms resulting from peripheral vascular reconstruction is presented. The analysis is divided into three 5-year periods. It is obvious from this presentation that the etiology is probably caused by suture material, end-to-side anastomosis, proximity of the anastomosis to a joint and intimectomy of the recipient artery at the original operative procedure. None of the lesions reported herein were associated with graft or wound sepsis. Anastomotic aneurysms at the proximal aortic suture line resulted in aortoenteric fistulae in 6 of 8 cases. There were no survivors  in these 6 cases despite successful graft replacement. It is to be emphasized that the complication of anastomotic aneurysm still remains a significant complication in peripheral vascular surgery with a 1.9% incidence noted in 320 operations performed from 1970-1974.", "contents": "Peripheral vascular anastomotic aneurysms: a fifteen-year experience. A 15-year experience with anastomotic aneurysms resulting from peripheral vascular reconstruction is presented. The analysis is divided into three 5-year periods. It is obvious from this presentation that the etiology is probably caused by suture material, end-to-side anastomosis, proximity of the anastomosis to a joint and intimectomy of the recipient artery at the original operative procedure. None of the lesions reported herein were associated with graft or wound sepsis. Anastomotic aneurysms at the proximal aortic suture line resulted in aortoenteric fistulae in 6 of 8 cases. There were no survivors  in these 6 cases despite successful graft replacement. It is to be emphasized that the complication of anastomotic aneurysm still remains a significant complication in peripheral vascular surgery with a 1.9% incidence noted in 320 operations performed from 1970-1974."}
{"id": "PMID:1082313", "title": "A controlled surgical approach to annulo-aortic ectasia.", "content": "Annulo-aortic ectasia has attracted much surgical attention in the last 20 years. Replacement of the aortic valve and ascending aorta from the valve ring to just proximal to the innominate artery eliminates most, if not all, the pathologically involved tissue. Composite valve-Dacron tube grafting, plus elective saphenous vein grafts from the coronary orifices to the Dacron tube or distal aortic wall, provide a safe systematic approach to this entity. A review of surgical techniques and a description of a successful case employing this method are presented.", "contents": "A controlled surgical approach to annulo-aortic ectasia. Annulo-aortic ectasia has attracted much surgical attention in the last 20 years. Replacement of the aortic valve and ascending aorta from the valve ring to just proximal to the innominate artery eliminates most, if not all, the pathologically involved tissue. Composite valve-Dacron tube grafting, plus elective saphenous vein grafts from the coronary orifices to the Dacron tube or distal aortic wall, provide a safe systematic approach to this entity. A review of surgical techniques and a description of a successful case employing this method are presented."}
{"id": "PMID:1082314", "title": "Coronary artery grafting with the saphenous vein or internal mammary artery. Comparison of late results in two consecutive series of patients.", "content": "In two series of consecutive patients who underwent coronary artery grafting with the saphenous vein or the internal mammary artery (IMA), angiographic studies were conducted two weeks and one year after operation. The early patency rate was 91.8% in 184 vein grafts and 97.4% in 38 IMA grafts. Late patency was 93.1% in 143 vein grafts and 90.9% in 33 IMA grafts. Thus, the cumulative patency rate at one year was 84.9% for vein grafts and 88.3% for IMA grafts. The incidence of severe narrowing at the site of anastomosis was 2.2% in vein grafts and 7.9% in IMA grafts; the rate of occlusion of the distal limb of the recipient coronary artery was, respectively, 4.3 and 7.9%. Diffuse reduction in the caliber of grafts at one year was less frequent with IMA grafts (12.7 versus 6.6%). On the other hand, blood flow was significantly higher in vein grafts: 67.4 versus 45.2 ml per minute (p less than 0.001). Blood flow in IMA grafts whose caliber was larger than that of the recipient coronary artery was significantly higher than flow in IMA grafts with a caliber equal or inferior to that of the coronary artery: 63.1 versus 32.7 ml per minute (p less than 0.001). Although cumulative patency rates at one year were comparable in both series, because only one-third of the IMAs were larger than their recipient coronary arteries, flow was somewhat restricted in the other two-thirds. This suggests that the IMA does not constitute the better bypass conduit and, unless it is of large caliber, preferably should be used in the grafting of small coronary arteries or those with limited runoff.", "contents": "Coronary artery grafting with the saphenous vein or internal mammary artery. Comparison of late results in two consecutive series of patients. In two series of consecutive patients who underwent coronary artery grafting with the saphenous vein or the internal mammary artery (IMA), angiographic studies were conducted two weeks and one year after operation. The early patency rate was 91.8% in 184 vein grafts and 97.4% in 38 IMA grafts. Late patency was 93.1% in 143 vein grafts and 90.9% in 33 IMA grafts. Thus, the cumulative patency rate at one year was 84.9% for vein grafts and 88.3% for IMA grafts. The incidence of severe narrowing at the site of anastomosis was 2.2% in vein grafts and 7.9% in IMA grafts; the rate of occlusion of the distal limb of the recipient coronary artery was, respectively, 4.3 and 7.9%. Diffuse reduction in the caliber of grafts at one year was less frequent with IMA grafts (12.7 versus 6.6%). On the other hand, blood flow was significantly higher in vein grafts: 67.4 versus 45.2 ml per minute (p less than 0.001). Blood flow in IMA grafts whose caliber was larger than that of the recipient coronary artery was significantly higher than flow in IMA grafts with a caliber equal or inferior to that of the coronary artery: 63.1 versus 32.7 ml per minute (p less than 0.001). Although cumulative patency rates at one year were comparable in both series, because only one-third of the IMAs were larger than their recipient coronary arteries, flow was somewhat restricted in the other two-thirds. This suggests that the IMA does not constitute the better bypass conduit and, unless it is of large caliber, preferably should be used in the grafting of small coronary arteries or those with limited runoff."}
{"id": "PMID:1082315", "title": "Comparative hemodynamic properties of vein and mammary artery in coronary bypass operations.", "content": "To understand better the observed differences in bypass flows between vein and internal mammary artery (IMA) grafts, a technique was devised for anastomosing both vein and IMA to the same anterior descending coronary artery in 14 patients. In the stable postperfusion state, flows in the two bypass conduits were simultaneously recorded as well as pressure relationships in both grafts and the left ventricle. The supply/demand ratio for left ventricular performance was calculated with respect to the diastolic pressure-time index/tension-time index (DPTI/TTI) for each bypass independently and simultaneously and then compared. The DPTI/TTI ratio was nearly two times greater with the vein bypass than with the IMA. This difference was further confirmed by the flow studies, in which blood flow through the vein ranged 2 to 3 times higher than IMA flow to the same coronary bed. By present criteria the DPTI/TTI ratio for IMA grafts to the left ventricle was inadequate in the majority of patients studied, and atrial pacing markedly lowered the DPTI/TTI ratio of the IMA. The choice of vein or IMA as a bypass is a critical determinant of the resultant bypass-left ventricular DPTI/TTI ratio. Vein bypasses exhibited far superior hemodynamic capability in the resting state, and the effect of atrial pacing on the DPTI/TTI ratio in IMA-vein-left ventricle bypasses confirms this point.", "contents": "Comparative hemodynamic properties of vein and mammary artery in coronary bypass operations. To understand better the observed differences in bypass flows between vein and internal mammary artery (IMA) grafts, a technique was devised for anastomosing both vein and IMA to the same anterior descending coronary artery in 14 patients. In the stable postperfusion state, flows in the two bypass conduits were simultaneously recorded as well as pressure relationships in both grafts and the left ventricle. The supply/demand ratio for left ventricular performance was calculated with respect to the diastolic pressure-time index/tension-time index (DPTI/TTI) for each bypass independently and simultaneously and then compared. The DPTI/TTI ratio was nearly two times greater with the vein bypass than with the IMA. This difference was further confirmed by the flow studies, in which blood flow through the vein ranged 2 to 3 times higher than IMA flow to the same coronary bed. By present criteria the DPTI/TTI ratio for IMA grafts to the left ventricle was inadequate in the majority of patients studied, and atrial pacing markedly lowered the DPTI/TTI ratio of the IMA. The choice of vein or IMA as a bypass is a critical determinant of the resultant bypass-left ventricular DPTI/TTI ratio. Vein bypasses exhibited far superior hemodynamic capability in the resting state, and the effect of atrial pacing on the DPTI/TTI ratio in IMA-vein-left ventricle bypasses confirms this point."}
{"id": "PMID:1082316", "title": "Intimal hyperplasia. A cause of radial artery aortocoronary bypass graft failure.", "content": "Autogenous radial artery grafts have been advocated for those situations in which adequate saphenous vein is not available for aortocoronary bypass procedures. It was anticipated that autogenous artery would demonstrate less predilection to develop the intimal proliferative changes seen with vein grafts in the arterial system. Early clinical experience with 79 patients receiving one or more radial artery grafts has shown that the radial artery is not spared occlusive intimal proliferative changes. Although early restudy of 6 patients was encouraging, late restudy in 29 patients showed 22 of 34 radial artery grafts (64.7%) to be unsatisfactory. Recovered grafts from 3 patients who required a second operation revealed severe generalized intimal hyperplasia. On the basis of this experience we no longer consider the radial artery an alternative conduit for aortocoronary bypass.", "contents": "Intimal hyperplasia. A cause of radial artery aortocoronary bypass graft failure. Autogenous radial artery grafts have been advocated for those situations in which adequate saphenous vein is not available for aortocoronary bypass procedures. It was anticipated that autogenous artery would demonstrate less predilection to develop the intimal proliferative changes seen with vein grafts in the arterial system. Early clinical experience with 79 patients receiving one or more radial artery grafts has shown that the radial artery is not spared occlusive intimal proliferative changes. Although early restudy of 6 patients was encouraging, late restudy in 29 patients showed 22 of 34 radial artery grafts (64.7%) to be unsatisfactory. Recovered grafts from 3 patients who required a second operation revealed severe generalized intimal hyperplasia. On the basis of this experience we no longer consider the radial artery an alternative conduit for aortocoronary bypass."}
{"id": "PMID:1082317", "title": "The structural study of the saphenous vein.", "content": "From November, 1971, to September, 1974, 1,179 patients received aortocoronary saphenous vein bypass grafts at the Cleveland Clinic Hospital. Segments of saphenous vein from each patient were sent for microscopical analysis. These vein segments were classified as normal or abnormal (phlebosclerotic). Four hundred ninety-six normal vein grafts in 295 patients were restudied and had a patency of 87.9%. One hundred forty-four abnormal vein grafts in 86 patients were restudied and showed 89.5% patency. This study suggests that histopathological identification of an abnormal (phlebosclerotic) vein segment does not constitute a determining factor as far as late patency is concerned in a vein segment that is not grossly sclerotic.", "contents": "The structural study of the saphenous vein. From November, 1971, to September, 1974, 1,179 patients received aortocoronary saphenous vein bypass grafts at the Cleveland Clinic Hospital. Segments of saphenous vein from each patient were sent for microscopical analysis. These vein segments were classified as normal or abnormal (phlebosclerotic). Four hundred ninety-six normal vein grafts in 295 patients were restudied and had a patency of 87.9%. One hundred forty-four abnormal vein grafts in 86 patients were restudied and showed 89.5% patency. This study suggests that histopathological identification of an abnormal (phlebosclerotic) vein segment does not constitute a determining factor as far as late patency is concerned in a vein segment that is not grossly sclerotic."}
{"id": "PMID:1082318", "title": "Ascending aorta insertion of the dual-chambered intraaortic balloon for counterpulsation during cardiac operations.", "content": "A patient with left ventricular power failure after aortocoronary saphenous vein bypass was successfully managed by intraaortic dual-chambered balloon counterpulsation through the ascending aorta. This insertion route reverses the orientation of the spherical distal chamber, which inflates early in diastole and partially occludes the aorta. This position achieved an effective augmentation pressure during left ventricular diastole and reduced arterial end-diastolic pressure considerably. The technique of insertion and events at operation and postoperatively are discussed.", "contents": "Ascending aorta insertion of the dual-chambered intraaortic balloon for counterpulsation during cardiac operations. A patient with left ventricular power failure after aortocoronary saphenous vein bypass was successfully managed by intraaortic dual-chambered balloon counterpulsation through the ascending aorta. This insertion route reverses the orientation of the spherical distal chamber, which inflates early in diastole and partially occludes the aorta. This position achieved an effective augmentation pressure during left ventricular diastole and reduced arterial end-diastolic pressure considerably. The technique of insertion and events at operation and postoperatively are discussed."}
{"id": "PMID:1082319", "title": "The lesser saphenous vein. An alternative graft for coronary revascularization.", "content": "In patients with surgically removed or varicose greater saphenous veins and atherosclerotic or small internal mammary arteries, or in patients requiring three or more coronary bypasses, the lesser saphenous vein can be considered for aortocoronary revascularization procedures. Its suitability can be demonstrated preoperatively by careful clinical examination and by venography. This report describes the successful use of the lesser saphenous vein in 3 patients, with long-term follow-up.", "contents": "The lesser saphenous vein. An alternative graft for coronary revascularization. In patients with surgically removed or varicose greater saphenous veins and atherosclerotic or small internal mammary arteries, or in patients requiring three or more coronary bypasses, the lesser saphenous vein can be considered for aortocoronary revascularization procedures. Its suitability can be demonstrated preoperatively by careful clinical examination and by venography. This report describes the successful use of the lesser saphenous vein in 3 patients, with long-term follow-up."}
{"id": "PMID:1082322", "title": "Improvement in forward coronary blood flow by using a reversed saphenous vein with a competent valve.", "content": "Reversed segments of saphenous vein have been the grafts of choice for aortocoronary bypass (ACB). Internal mammary arteries and free radial autografts have recently been shown to have a higher ppatency rate, but flow is usually lower. Normally forward coronary blood flow ceases and retrograde flow occurs during cardiac systole. Prevention of retrograde flow with a rapidly acting valve proximal to the coronary artery anastomosis should improve forward coronary blood flow. Thirty-nine reversed saphenous veins containing a competent valve were implanted in 32 patients undergoing ACB. After accurate zero flow was determined and a resting state achieved, mean (22 veins) and pulsatile (17 veins) flows were measured distal to the valve with the valve competent and temporarily incompetent. A competent valve in 10 right coronary artery vein grafts increased mean flow by 29.7% (+41 ml/min; p less than 0.005) and pulsatile flow in 6 veins by 17.7% (p less than 0.001). A competent valve in 12 left coronary artery vein grafts increased mean flow by 31.3% (+34 ml/min; p less than 0.01) and pulsatile flow in 11 veins by 13.7% (p less than 0.001). This study suggests that a portion of reversed saphenous vein containing a competent valve provides greater coronary artery blood flow than veins without valves and may be the conduit of choice for coronary artery revascularization.", "contents": "Improvement in forward coronary blood flow by using a reversed saphenous vein with a competent valve. Reversed segments of saphenous vein have been the grafts of choice for aortocoronary bypass (ACB). Internal mammary arteries and free radial autografts have recently been shown to have a higher ppatency rate, but flow is usually lower. Normally forward coronary blood flow ceases and retrograde flow occurs during cardiac systole. Prevention of retrograde flow with a rapidly acting valve proximal to the coronary artery anastomosis should improve forward coronary blood flow. Thirty-nine reversed saphenous veins containing a competent valve were implanted in 32 patients undergoing ACB. After accurate zero flow was determined and a resting state achieved, mean (22 veins) and pulsatile (17 veins) flows were measured distal to the valve with the valve competent and temporarily incompetent. A competent valve in 10 right coronary artery vein grafts increased mean flow by 29.7% (+41 ml/min; p less than 0.005) and pulsatile flow in 6 veins by 17.7% (p less than 0.001). A competent valve in 12 left coronary artery vein grafts increased mean flow by 31.3% (+34 ml/min; p less than 0.01) and pulsatile flow in 11 veins by 13.7% (p less than 0.001). This study suggests that a portion of reversed saphenous vein containing a competent valve provides greater coronary artery blood flow than veins without valves and may be the conduit of choice for coronary artery revascularization."}
{"id": "PMID:1082323", "title": "A useful instrument for aortocoronary bypass operations.", "content": "A punch biopsy forceps is described that is used to make openings in the aorta for anastomosing one end of a saphenous vein graft to the ascending aorta. It can be very successful in myocardial revascularization when there is severe calcification of the aortic wall.", "contents": "A useful instrument for aortocoronary bypass operations. A punch biopsy forceps is described that is used to make openings in the aorta for anastomosing one end of a saphenous vein graft to the ascending aorta. It can be very successful in myocardial revascularization when there is severe calcification of the aortic wall."}
{"id": "PMID:1082325", "title": "Coexistent lymphoid interstitial pneumonia, pernicious anemia, and agammaglobulinemia.", "content": "Immunologic factors have been incriminated in the pathogenesis of lymphoid interstitial pneumonia. The discovery of a patient with coexistent lymphoid interestitial pneumonia, pernicious anemia, and common variable hypogammaglobulinemia focused attention on the possible autoimmune nature of this pulmonary disease. Extensive immunologic studies demonstrated a noticeably impaired bonemarrow-dependent (B cell) system and intact thymus-dependent (T cell) system. No evidence of humoral or cellular hypersensitivity to homologous lung determinants was found.", "contents": "Coexistent lymphoid interstitial pneumonia, pernicious anemia, and agammaglobulinemia. Immunologic factors have been incriminated in the pathogenesis of lymphoid interstitial pneumonia. The discovery of a patient with coexistent lymphoid interestitial pneumonia, pernicious anemia, and common variable hypogammaglobulinemia focused attention on the possible autoimmune nature of this pulmonary disease. Extensive immunologic studies demonstrated a noticeably impaired bonemarrow-dependent (B cell) system and intact thymus-dependent (T cell) system. No evidence of humoral or cellular hypersensitivity to homologous lung determinants was found."}
{"id": "PMID:1082328", "title": "[Plasminogen activator and other trypsin-like proteases in the uterus wall and their participation on the tissue bleeding (author's transl)].", "content": "In the myometrium and endometrium a content of plasminogen activator and non-specific trypsin-like proteases was determined. It was ascertained that there was a higher level of plasminogen activator in the endometrium during the menstruation in the contrary to the first and second phase of the menstrual cycle and that both plasminogen activator and non-specific trypsin-like proteases were relative higher in Corpusmyometrium than those Cervixmyometrium. The proteases present in the fractions of myometriumeluate were able to split casein and partially fibrin, too. These activities were not inhibited by epsilon aminocaproic acid and aprotinin. The importance of these findings for gynaecological bleeding was suggested.", "contents": "[Plasminogen activator and other trypsin-like proteases in the uterus wall and their participation on the tissue bleeding (author's transl)]. In the myometrium and endometrium a content of plasminogen activator and non-specific trypsin-like proteases was determined. It was ascertained that there was a higher level of plasminogen activator in the endometrium during the menstruation in the contrary to the first and second phase of the menstrual cycle and that both plasminogen activator and non-specific trypsin-like proteases were relative higher in Corpusmyometrium than those Cervixmyometrium. The proteases present in the fractions of myometriumeluate were able to split casein and partially fibrin, too. These activities were not inhibited by epsilon aminocaproic acid and aprotinin. The importance of these findings for gynaecological bleeding was suggested."}
{"id": "PMID:1082329", "title": "Infection of human and rhesus lymphoblastoid cells with Herpesvirus macaca.", "content": "The interaction between Herpesvirus macaca and lymphoblastoid cells grown in continuous culture, or purified from rhesus blood, was studied as a model for latent or chronic herpesvirus infection of leukocytes. Continuous lymphoblastoid cell lines infected in vitro were PA3, a human cell line with bone-marrow derived (B)-lymphocyte characteristics; LM/DM, a rhesus cell line with B-lymphocyte characteristics; and MOLT-4, a human cell line with thymus-derived (T)-lymphocyte characteristics. A distinct pattern of interaction was found for each lymphoblastoid cell line. A small but stable fraction of cells continued to express virus-specific antigens for more than three weeks following infection of the B-type lymphoblastoid cell lines of rhesus and human origin. Herpesvirus macaca replicated only in PA3 cells. Viral replication did not occur and viral antigens were not detected in either peripheral lymphocytes or MOLT-4 cells. The observations suggest that Herpesvirus macaca interacts in distinct patterns with different lymphoid cell lines in vitro, and that within a given culture, subpopulations of lymphoid cells are present at a given time which respond to the virus differently.", "contents": "Infection of human and rhesus lymphoblastoid cells with Herpesvirus macaca. The interaction between Herpesvirus macaca and lymphoblastoid cells grown in continuous culture, or purified from rhesus blood, was studied as a model for latent or chronic herpesvirus infection of leukocytes. Continuous lymphoblastoid cell lines infected in vitro were PA3, a human cell line with bone-marrow derived (B)-lymphocyte characteristics; LM/DM, a rhesus cell line with B-lymphocyte characteristics; and MOLT-4, a human cell line with thymus-derived (T)-lymphocyte characteristics. A distinct pattern of interaction was found for each lymphoblastoid cell line. A small but stable fraction of cells continued to express virus-specific antigens for more than three weeks following infection of the B-type lymphoblastoid cell lines of rhesus and human origin. Herpesvirus macaca replicated only in PA3 cells. Viral replication did not occur and viral antigens were not detected in either peripheral lymphocytes or MOLT-4 cells. The observations suggest that Herpesvirus macaca interacts in distinct patterns with different lymphoid cell lines in vitro, and that within a given culture, subpopulations of lymphoid cells are present at a given time which respond to the virus differently."}
{"id": "PMID:1082330", "title": "Malignant fibrous xanthoma with metastasis to cerebellopontine angle.", "content": "Malignant fibrous xanthoma is an uncommon tumor of histiocytic origin. It is a difficult tumor to diagnose, and may have an unpredictable and confusing clinical course. The malignant fibrous xanthoma described herein probably represents the first reported case of metastasis to the cerebellopontine angle. When properly diagnosed, the preferred treatment is wide excision. Metastases are usually to the lungs and regional lymph nodes, and portend a poor prognosis. Radiotherapy appears to be of little value.", "contents": "Malignant fibrous xanthoma with metastasis to cerebellopontine angle. Malignant fibrous xanthoma is an uncommon tumor of histiocytic origin. It is a difficult tumor to diagnose, and may have an unpredictable and confusing clinical course. The malignant fibrous xanthoma described herein probably represents the first reported case of metastasis to the cerebellopontine angle. When properly diagnosed, the preferred treatment is wide excision. Metastases are usually to the lungs and regional lymph nodes, and portend a poor prognosis. Radiotherapy appears to be of little value."}
{"id": "PMID:1082331", "title": "The results of diagnostic colonoscopy in the management of unexplained bleeding from the rectum.", "content": "A consecutive series of 71 colonoscopies performed for unexplained bleeding per rectum is reported. All patients had had previous barium enema examinations reported as normal. In 26 colonoscopies lesions were detected; there were 12 cancers and 11 polyps. Twenty-five of these lesions were in the left colon. These findings illustrate the value of colonoscopy in supplementing the available radiological findings. The sighting of blood at sigmoidoscopy was associated with a lesion found on colonoscopy in most instances, whereas the presence of occult blood was associated with a lesion in very few. Colonoscopy should be considered prior to laparotomy in every case of unexplained blood in the stools.", "contents": "The results of diagnostic colonoscopy in the management of unexplained bleeding from the rectum. A consecutive series of 71 colonoscopies performed for unexplained bleeding per rectum is reported. All patients had had previous barium enema examinations reported as normal. In 26 colonoscopies lesions were detected; there were 12 cancers and 11 polyps. Twenty-five of these lesions were in the left colon. These findings illustrate the value of colonoscopy in supplementing the available radiological findings. The sighting of blood at sigmoidoscopy was associated with a lesion found on colonoscopy in most instances, whereas the presence of occult blood was associated with a lesion in very few. Colonoscopy should be considered prior to laparotomy in every case of unexplained blood in the stools."}
{"id": "PMID:1082332", "title": "Vestibular-ocular accommodation reflex in man.", "content": "Stimulation of the vestibular system by angular acceleration produces widespread sensory and motor effects. The present study was designed to study a motor effect which has not been reported in the literature, i.e., the influence of rotary acceleration of the body on ocular accommodation. The accommodation of 10 young men was recorded before and after a high-level deceleration to zero velocity following 30 s of rotation. Accommodation was recorded continuously on an infrared optometer for 110 s under two conditions; while the subjects observed a target set at the far point, and while they viewed the same target through a 0.3-mm pinhole. Stimulation by high-level rotary deceleration produced positive accommodation or a pseudomyopia under both conditions, but the positive accommodation was substantially greater and lasted much longer during fixation through the pinhole. It is hypothesized that this increase in accommodation is a result of a vestibular-ocular accommodation reflex.", "contents": "Vestibular-ocular accommodation reflex in man. Stimulation of the vestibular system by angular acceleration produces widespread sensory and motor effects. The present study was designed to study a motor effect which has not been reported in the literature, i.e., the influence of rotary acceleration of the body on ocular accommodation. The accommodation of 10 young men was recorded before and after a high-level deceleration to zero velocity following 30 s of rotation. Accommodation was recorded continuously on an infrared optometer for 110 s under two conditions; while the subjects observed a target set at the far point, and while they viewed the same target through a 0.3-mm pinhole. Stimulation by high-level rotary deceleration produced positive accommodation or a pseudomyopia under both conditions, but the positive accommodation was substantially greater and lasted much longer during fixation through the pinhole. It is hypothesized that this increase in accommodation is a result of a vestibular-ocular accommodation reflex."}
{"id": "PMID:1082334", "title": "Animal experimental studies on chronic granulomatous inflammation and T-lymphocyte-system.", "content": "The presence of lymphocytes in chronic granulomatous inflammations is frequently thought to indicate that thymus-dependent cellular immune mechanisms are involved in the pathogenesis of such processes. Therefore, in the present studies, a model of a granulomatous heaptitis (induced by heat-inactivated group A streptococci) was used to determine whether liver granulomata, consisting of macrophages and lymphocytes, could also be evoked in neonatally thymectomized or in congenitally thymus-deficient nude mice. The morphological (light and electron microscopical, immunohistological) investigations were supplemented with selective determination of T- and B-lymphocyte function. The thymus-deficient mice, after injection of streptococci, developed liver granulomata that did differ neither quanlitatively nor quantitatively from those of control animals with thymus. Lymphocytes were found within the granulomata in both animal groups. There was no evidence for functional disorder of the RES in thymus-deficient mice; on the contrary, RES-activity seemed to be increased. Phagocytosis of streptococci, their intracellular breakdown and streptococcal antigen-degradation occurred as fast or faster in such animals. PHA- and LPS-stimulation of spleen lymphocytes indicated a considerable depletion of T-cells in neonatally thymectomized mice and a complete absence of T-cells in congenitally thymus-deficient nude mice. However, radioimmunological determination of antibodies to group A streptococcal carbohydrate revealed that both groups of experimental animals possessed functionally active B-cells. Therefore, the granulomatous hepatitis described here can be defined as a focal reaction of the liver RES with \"B-lymphocyte trapping\". Cellular immune mechanisms are not involved in the pathogenesis of these lesions.", "contents": "Animal experimental studies on chronic granulomatous inflammation and T-lymphocyte-system. The presence of lymphocytes in chronic granulomatous inflammations is frequently thought to indicate that thymus-dependent cellular immune mechanisms are involved in the pathogenesis of such processes. Therefore, in the present studies, a model of a granulomatous heaptitis (induced by heat-inactivated group A streptococci) was used to determine whether liver granulomata, consisting of macrophages and lymphocytes, could also be evoked in neonatally thymectomized or in congenitally thymus-deficient nude mice. The morphological (light and electron microscopical, immunohistological) investigations were supplemented with selective determination of T- and B-lymphocyte function. The thymus-deficient mice, after injection of streptococci, developed liver granulomata that did differ neither quanlitatively nor quantitatively from those of control animals with thymus. Lymphocytes were found within the granulomata in both animal groups. There was no evidence for functional disorder of the RES in thymus-deficient mice; on the contrary, RES-activity seemed to be increased. Phagocytosis of streptococci, their intracellular breakdown and streptococcal antigen-degradation occurred as fast or faster in such animals. PHA- and LPS-stimulation of spleen lymphocytes indicated a considerable depletion of T-cells in neonatally thymectomized mice and a complete absence of T-cells in congenitally thymus-deficient nude mice. However, radioimmunological determination of antibodies to group A streptococcal carbohydrate revealed that both groups of experimental animals possessed functionally active B-cells. Therefore, the granulomatous hepatitis described here can be defined as a focal reaction of the liver RES with \"B-lymphocyte trapping\". Cellular immune mechanisms are not involved in the pathogenesis of these lesions."}
{"id": "PMID:1082341", "title": "Studies on the humoral regulation of granulopoiesis in leukaemic RFM mice.", "content": "Intraperitoneal diffusion chambers have been used to investigate changes in humoral factors during the development of myeloid leukaemia in mice. Normal mouse bone marrow cells form colonies of granulocytes and macrophages when cultured in semi-solid agar medium within intraperitoneal diffusion chambers. The use of mice bearing transplanted myeloid leukaemia as Agar Diffusion Chamber (ADC) hosts enhances colony formation from normal marrow. The humoral basis for this stimulation has been shown by the colony stimulating activity of the fluid entering the diffusion chambers when assayed against normal mouse bone marrow cells in agar culture in vitro. The stimulus to colony growth in ADCs and the in vitro colony stimulating activity depend on the phase in the development of the leukaemia investigated, and the stimulation was abolished by splenectomy. There was no apparent relationship between the growth of the leukaemic cell population in vivo and the level of the stimulating factor detected in leukaemic mice.", "contents": "Studies on the humoral regulation of granulopoiesis in leukaemic RFM mice. Intraperitoneal diffusion chambers have been used to investigate changes in humoral factors during the development of myeloid leukaemia in mice. Normal mouse bone marrow cells form colonies of granulocytes and macrophages when cultured in semi-solid agar medium within intraperitoneal diffusion chambers. The use of mice bearing transplanted myeloid leukaemia as Agar Diffusion Chamber (ADC) hosts enhances colony formation from normal marrow. The humoral basis for this stimulation has been shown by the colony stimulating activity of the fluid entering the diffusion chambers when assayed against normal mouse bone marrow cells in agar culture in vitro. The stimulus to colony growth in ADCs and the in vitro colony stimulating activity depend on the phase in the development of the leukaemia investigated, and the stimulation was abolished by splenectomy. There was no apparent relationship between the growth of the leukaemic cell population in vivo and the level of the stimulating factor detected in leukaemic mice."}
{"id": "PMID:1082338", "title": "[Prospective study of patients with prolonged fever].", "content": "A prospective study was made in 283 patients who attended IMAN's Children's Hospital, with fever the main symptom. A clinical and paraclinical procedure was designed for the study of each patient. 112 patients were eliminated because they did not follow the established criteria. All patients had acute infectious diseases considered trivial; 85% were 3 weeks to 2 years of age. They all had an antibacterial treatment without precise diagnosis. It was considered that on admission the patients showed a normal course in the natural history of the basic disease. The study group included 171 patients 2 months to 13 years of age; 62.5% had fever due to infection, 12.2% to collagenopathies, 7% to neoplasias 5.2% to miscellaneous causes and 12.8% were not diagnosed. The most common infectious causes for prolonged fever were tuberculosis, upper respiratory infections, amoebic liver abscess, typhoid fever and malaria. Careful questioning and clinical examination were enough to enlighten diagnosis in more than 80% of the patients.", "contents": "[Prospective study of patients with prolonged fever]. A prospective study was made in 283 patients who attended IMAN's Children's Hospital, with fever the main symptom. A clinical and paraclinical procedure was designed for the study of each patient. 112 patients were eliminated because they did not follow the established criteria. All patients had acute infectious diseases considered trivial; 85% were 3 weeks to 2 years of age. They all had an antibacterial treatment without precise diagnosis. It was considered that on admission the patients showed a normal course in the natural history of the basic disease. The study group included 171 patients 2 months to 13 years of age; 62.5% had fever due to infection, 12.2% to collagenopathies, 7% to neoplasias 5.2% to miscellaneous causes and 12.8% were not diagnosed. The most common infectious causes for prolonged fever were tuberculosis, upper respiratory infections, amoebic liver abscess, typhoid fever and malaria. Careful questioning and clinical examination were enough to enlighten diagnosis in more than 80% of the patients."}
{"id": "PMID:1082339", "title": "[Histiocytosis. Clinical aspects in childhood].", "content": "The clinical and pathologic features in 11 cases of histiocytosis were reviewed. The were 6 cases of Hand-Sch\u00fcller-Christian, disease 4 cases of Letterer-Siwe and 1 case of eosinophilic granuloma. The clinical differences among those diseases are outlined. We agree with other authors that the symptoms are related in great measure to the moment of embryonic differentation in which the proliferation occurred. When proliferation is present at an early stage of development of macrophage (promonocyte), a more malignant disease appears than when it occurs at a later stage (macrophage). The treatment of histiocytosis is briefly outlined.", "contents": "[Histiocytosis. Clinical aspects in childhood]. The clinical and pathologic features in 11 cases of histiocytosis were reviewed. The were 6 cases of Hand-Sch\u00fcller-Christian, disease 4 cases of Letterer-Siwe and 1 case of eosinophilic granuloma. The clinical differences among those diseases are outlined. We agree with other authors that the symptoms are related in great measure to the moment of embryonic differentation in which the proliferation occurred. When proliferation is present at an early stage of development of macrophage (promonocyte), a more malignant disease appears than when it occurs at a later stage (macrophage). The treatment of histiocytosis is briefly outlined."}
{"id": "PMID:1082343", "title": "Factors influencing prognosis in adults with acute myelogenous leukaemia.", "content": "A study of the thymidine labelling index (TLI) of bone marrow blast cells in 58 untreated patients with acute myelogenous leukemia showed no correlation with remission rate but there was a strong correlation between labelling index and remission length in the 21 patients who achieved remission. The median remission length of the patients was 33 weeks. Of the 12 patients with initial labelling indices greater than 10%, only 2 had remissions longer than 33 weeks whereas 8 of the 9 patients with labelling indices less than 10% had remissions longer than 33 weeks. No correlation could be found between the degree of cytological differentiation and remission induction, remission length or survival. No correlation was found between the TLI and the degree of cytological differentiation. Age and initial platelet count were confirmed to be important factors influencing complete remission rate, but these factors did not correlate with remission length. Sixteen patients had their pretreatment sera assayed for mouse marrow colony stimulating activity and inhibitor levels but there was no correlation with subsequent response to treatment, although the number of patients examined was clearly too small for any definite conclusions to be drawn.", "contents": "Factors influencing prognosis in adults with acute myelogenous leukaemia. A study of the thymidine labelling index (TLI) of bone marrow blast cells in 58 untreated patients with acute myelogenous leukemia showed no correlation with remission rate but there was a strong correlation between labelling index and remission length in the 21 patients who achieved remission. The median remission length of the patients was 33 weeks. Of the 12 patients with initial labelling indices greater than 10%, only 2 had remissions longer than 33 weeks whereas 8 of the 9 patients with labelling indices less than 10% had remissions longer than 33 weeks. No correlation could be found between the degree of cytological differentiation and remission induction, remission length or survival. No correlation was found between the TLI and the degree of cytological differentiation. Age and initial platelet count were confirmed to be important factors influencing complete remission rate, but these factors did not correlate with remission length. Sixteen patients had their pretreatment sera assayed for mouse marrow colony stimulating activity and inhibitor levels but there was no correlation with subsequent response to treatment, although the number of patients examined was clearly too small for any definite conclusions to be drawn."}
{"id": "PMID:1082344", "title": "Active specific immunotherapy of mouse methylcholanthrene induced tumours with Corynebacterium parvum and irradiated tumour cells.", "content": "The relative efficiency of active nonspecific or specific immunotherapy of developing methylcholanthrene induced fibrosarcomata with C. parvum was compared. For nonspecific immunotherapy, mice were challenged with tumour cells s.c. or i.v., and 2 days later injected i.v. with dilutions of C. parvum. The only significant effect was a retardation of s.c. tumour growth by the highest concentration of C. parvum (350 mug). However, active specific immunotherapy, using mixtures of C. parvum and irradiated or living tumour cells in the footpads, suppressed tumour growth when given at 2 or 6, but not 10, days after tumour challenge. Successful therapy required: sufficient tumour cells (greater than or equal to 5 X 10(4)); an optimal dose of C. parvum (5-120 mug, increasing with the number of tumour cells); an intact T cell system; the same tumour cells for challenge and treatment. The specificity was confirmed in a protection system in which treatment was given 7 days before tumour challenge. No protective immunity could be achieved with mixtures of C. parvum and foetal cells. Thus in this system C. parvum potentiates protective immunity only to the tumour unique TSTA.", "contents": "Active specific immunotherapy of mouse methylcholanthrene induced tumours with Corynebacterium parvum and irradiated tumour cells. The relative efficiency of active nonspecific or specific immunotherapy of developing methylcholanthrene induced fibrosarcomata with C. parvum was compared. For nonspecific immunotherapy, mice were challenged with tumour cells s.c. or i.v., and 2 days later injected i.v. with dilutions of C. parvum. The only significant effect was a retardation of s.c. tumour growth by the highest concentration of C. parvum (350 mug). However, active specific immunotherapy, using mixtures of C. parvum and irradiated or living tumour cells in the footpads, suppressed tumour growth when given at 2 or 6, but not 10, days after tumour challenge. Successful therapy required: sufficient tumour cells (greater than or equal to 5 X 10(4)); an optimal dose of C. parvum (5-120 mug, increasing with the number of tumour cells); an intact T cell system; the same tumour cells for challenge and treatment. The specificity was confirmed in a protection system in which treatment was given 7 days before tumour challenge. No protective immunity could be achieved with mixtures of C. parvum and foetal cells. Thus in this system C. parvum potentiates protective immunity only to the tumour unique TSTA."}
{"id": "PMID:1082346", "title": "Human leukocyte granule elastase: rapid isolation and characterization.", "content": "Human granulocytic elastases have been purified by a two-step procedure involving affinity chromatography of crude extracts of leukocytic granules on Sepharose-Trasylol, followed by ion-exchange chromatography on CM-cellulose to resolve the isoelastases. All of these enzymes were found to be glycoproteins with the carbohydrate content of the major form being composed essentially of only neutral sugars. The molecular weight of this form was found to be near 30 000 daltons with the other forms being slightly higher. Preliminary structural analyses indicate that all of the elastase isozymes have identical NH2-terminal sequences suggesting that the differences in mobility of the four proteins are not due to different degrees of activation from a common zymogen but, more likely, from minor changes in carbohydrate content. Human granulocytic elastases are less active on ligament elastin than porcine pancreatic elastase, but both are inhibited by synthetic elastase active-site directed low molecular weight compounds (Tuhy, P. M., and Powers, J. C. (1975), FEBS Lett. 50, 359) as well as by plasma alpha-1-proteinase inhibitor (formerly called alpha-1-antitrypsin). In the latter case a stable complex with mol wt of 78 000 daltons is formed indicating the formation of a 1:1 complex.", "contents": "Human leukocyte granule elastase: rapid isolation and characterization. Human granulocytic elastases have been purified by a two-step procedure involving affinity chromatography of crude extracts of leukocytic granules on Sepharose-Trasylol, followed by ion-exchange chromatography on CM-cellulose to resolve the isoelastases. All of these enzymes were found to be glycoproteins with the carbohydrate content of the major form being composed essentially of only neutral sugars. The molecular weight of this form was found to be near 30 000 daltons with the other forms being slightly higher. Preliminary structural analyses indicate that all of the elastase isozymes have identical NH2-terminal sequences suggesting that the differences in mobility of the four proteins are not due to different degrees of activation from a common zymogen but, more likely, from minor changes in carbohydrate content. Human granulocytic elastases are less active on ligament elastin than porcine pancreatic elastase, but both are inhibited by synthetic elastase active-site directed low molecular weight compounds (Tuhy, P. M., and Powers, J. C. (1975), FEBS Lett. 50, 359) as well as by plasma alpha-1-proteinase inhibitor (formerly called alpha-1-antitrypsin). In the latter case a stable complex with mol wt of 78 000 daltons is formed indicating the formation of a 1:1 complex."}
{"id": "PMID:1082347", "title": "The action of cordycepin on nascent nuclear RNA and poly(A) synthesis in regenerating liver.", "content": "Following a 5 min pulse of [5- 3H]orotic acid via the protal vein, the specific radioactivity of non-poly(A)heterogeneous nuclear RNA (HnRNA) reaches a peak at 12 h after partial hepatectomy. In contrast, poly(A)-HnRNA was maximally elevated only at 2 h after operation. After intraportal injection of cordycepin (3'-deoxyadenosine) 1 min before [5-3H]orotic acid, a dose-dependent inhibition of nuclear HnRNA and rRNA occurred. Fractionation of HnRNA on poly(U)-Sepharose following 20 mg/kg of cordycepin revealed that a 65% reduction occurred in the labeling of poly(A)-HnRNA while non-polyactivity of UTP in control and cordycepin-treated animals indicated no significant alterations in these parameters. Assessment of poly(A) size using poly(A)-HnRNA annealed with oligo(dT)10 as template primer for Escherichia coli DNA polymerase I, showed that 20 mg/kg of cordycepin inhibited nuclear polyadenylylation by 43%; no alteration in the binding of poly(A)-HnRNA to Millipore filters occurred at this dose of cordycepin. These results indicate that cordycepin is a non-selective inhibitor of nuclear RNA and poly(A)synthesis in regenerating rat liver.", "contents": "The action of cordycepin on nascent nuclear RNA and poly(A) synthesis in regenerating liver. Following a 5 min pulse of [5- 3H]orotic acid via the protal vein, the specific radioactivity of non-poly(A)heterogeneous nuclear RNA (HnRNA) reaches a peak at 12 h after partial hepatectomy. In contrast, poly(A)-HnRNA was maximally elevated only at 2 h after operation. After intraportal injection of cordycepin (3'-deoxyadenosine) 1 min before [5-3H]orotic acid, a dose-dependent inhibition of nuclear HnRNA and rRNA occurred. Fractionation of HnRNA on poly(U)-Sepharose following 20 mg/kg of cordycepin revealed that a 65% reduction occurred in the labeling of poly(A)-HnRNA while non-polyactivity of UTP in control and cordycepin-treated animals indicated no significant alterations in these parameters. Assessment of poly(A) size using poly(A)-HnRNA annealed with oligo(dT)10 as template primer for Escherichia coli DNA polymerase I, showed that 20 mg/kg of cordycepin inhibited nuclear polyadenylylation by 43%; no alteration in the binding of poly(A)-HnRNA to Millipore filters occurred at this dose of cordycepin. These results indicate that cordycepin is a non-selective inhibitor of nuclear RNA and poly(A)synthesis in regenerating rat liver."}
{"id": "PMID:1082348", "title": "Deviations from the flux ratio equation for chloride ions in ouabain- and acetazolamide-treated frog skin.", "content": "In order to establish whether or not chloride ions behave as freely moving particles in \"passive\", i.e. ouabain- and acetazolamide-treated, frog skin, tracer fluxes of 36Cl-have been measured while a voltage (generally +40 mV, serosal side positive) across the skin was applied. Ussing's flux ratio equation has been used as a criterion for this type of transport. One group of skin samples exhibited significant exchange diffusion phenomena. Most samples in a second group either behaved according to the flux ratio equation of showed significant and extreme exchange diffusion. From flux ratios obtained at two different voltages across various skin samples, showing extreme exchange diffusion, it appeared that the simple form of Kedem and Essig's law derived from irreversible thermodynamics, which is valid for homogeneous systems, does not apply to the type of exchange diffusion found. The system can, however, be described by a 1:1 exchange mechanism working in parallel with a diffusional pathway. The ratio exchange flux/observed efflux must then have a constant value (0.83) at the voltages appled, which implies that the exchange flux is voltage dependent. By comparison with iodide flux experiments as carried out by Ussing, it is shown that iodide exhibits the same type of exchange diffusion. A carrier, possibly responsible for the observed behaviour, is described.", "contents": "Deviations from the flux ratio equation for chloride ions in ouabain- and acetazolamide-treated frog skin. In order to establish whether or not chloride ions behave as freely moving particles in \"passive\", i.e. ouabain- and acetazolamide-treated, frog skin, tracer fluxes of 36Cl-have been measured while a voltage (generally +40 mV, serosal side positive) across the skin was applied. Ussing's flux ratio equation has been used as a criterion for this type of transport. One group of skin samples exhibited significant exchange diffusion phenomena. Most samples in a second group either behaved according to the flux ratio equation of showed significant and extreme exchange diffusion. From flux ratios obtained at two different voltages across various skin samples, showing extreme exchange diffusion, it appeared that the simple form of Kedem and Essig's law derived from irreversible thermodynamics, which is valid for homogeneous systems, does not apply to the type of exchange diffusion found. The system can, however, be described by a 1:1 exchange mechanism working in parallel with a diffusional pathway. The ratio exchange flux/observed efflux must then have a constant value (0.83) at the voltages appled, which implies that the exchange flux is voltage dependent. By comparison with iodide flux experiments as carried out by Ussing, it is shown that iodide exhibits the same type of exchange diffusion. A carrier, possibly responsible for the observed behaviour, is described."}
{"id": "PMID:1082349", "title": "The influence of glutamine, its decomposition products, and glutaminase on the transformation of human and mouse lymphocytes.", "content": "The extent of blast transformation for human and BALB/c mouse lymphocytes has been examined over a wide range of glutamine concentrations with several agents which initiate blastogenesis. Maximum [3H] thymidine incorporation was seen at 0.5 mM glutamine for lymphoid tissues stimulated in the following manner: human and BALB/c splenic and peripheral blood lymphocytes with phytohemagglutinin, BALB/c splenic lymphocytes with lipopolysaccharide, and BALB/c vs C3H/HeJ two-way mixed lymphocyte cultures. The inhibition of blastogenesis exerted by glutamine concentrations greater than 0.5 mM could not be reversed by washing and reculturing the cells at 0.5 mM glutamine. To elucidate the reason for inhibition by higher glutamine concentrations, the products of spontaneous glutamine decomposition, L-2-pyrrolidone-5-carboxylic acid and ammonia were tested for their in vitro influence on BALB/c splenocyte blastogenesis. Pyrrolidone-carboxylic acid, in concentrations up to 5 mM, was without effect. In contrast, ammonia concentrations exceeding 1 mM became increasingly more inhibitory. The genesis of inhibitory levels of ammonia in culture medium was confirmed and has been considered as primarily responsible for inhibiton by high glutamine. Addition of Escherichia coli glutaminase (pH optimum 4.9) to cultures of BALB/c splenocytes or human peripheral blood lymphocytes had no effect on either the extent of blastogenesis of these tissues or the glutamine levels in their culture medium.", "contents": "The influence of glutamine, its decomposition products, and glutaminase on the transformation of human and mouse lymphocytes. The extent of blast transformation for human and BALB/c mouse lymphocytes has been examined over a wide range of glutamine concentrations with several agents which initiate blastogenesis. Maximum [3H] thymidine incorporation was seen at 0.5 mM glutamine for lymphoid tissues stimulated in the following manner: human and BALB/c splenic and peripheral blood lymphocytes with phytohemagglutinin, BALB/c splenic lymphocytes with lipopolysaccharide, and BALB/c vs C3H/HeJ two-way mixed lymphocyte cultures. The inhibition of blastogenesis exerted by glutamine concentrations greater than 0.5 mM could not be reversed by washing and reculturing the cells at 0.5 mM glutamine. To elucidate the reason for inhibition by higher glutamine concentrations, the products of spontaneous glutamine decomposition, L-2-pyrrolidone-5-carboxylic acid and ammonia were tested for their in vitro influence on BALB/c splenocyte blastogenesis. Pyrrolidone-carboxylic acid, in concentrations up to 5 mM, was without effect. In contrast, ammonia concentrations exceeding 1 mM became increasingly more inhibitory. The genesis of inhibitory levels of ammonia in culture medium was confirmed and has been considered as primarily responsible for inhibiton by high glutamine. Addition of Escherichia coli glutaminase (pH optimum 4.9) to cultures of BALB/c splenocytes or human peripheral blood lymphocytes had no effect on either the extent of blastogenesis of these tissues or the glutamine levels in their culture medium."}
{"id": "PMID:1082350", "title": "Effects of cycloheximide on protein degradation and gluconeogenesis in the perfused rat liver.", "content": "Cycloheximide at concentrations above 18 muM produced a 93% inhibition of total protein synthesis measured by valine incorporation in the perfused rat liver. Rates of protein degradation were estimated by perfusing livers prelabeled in vivo with L-[1-14C]valine with medium containing 15 mM L-valine. Thus labeled valine released from liver protein during perfusion was greatly diluted and reincorporation of label was minimized. Cycloheximide at 18 muM inhibited protein degradation by over 60%, after a delay of 15-20 min. Associated with these effects were dose-dependent increases in the rates of glucose and urea production. Glucose production increased 3 fold, from 0.54 +/- 0.07  in control to 1.85 +/- 0.24 mumol/min/100 g rat in cycloheximide-treated livers. Urea production increased from 0.24 +/- 0.02 to 0.62 +/- 0.06 mumol/min/100 g rat. No changes in liver glycogen or cyclic AMP content were seen. The data suggest that inhibition of protein synthesis provides an increased availability of intra-cellular amino acids and that many of these are rapidly degraded, yielding urea and glucose. This is supported by the fact that intracellular alanine levels were significantly increased following cycloheximide treatment. It is possible that the inhibition of protein degradation by cycloheximide is due to altered intra-cellular pools of amino acids or their metabolites.", "contents": "Effects of cycloheximide on protein degradation and gluconeogenesis in the perfused rat liver. Cycloheximide at concentrations above 18 muM produced a 93% inhibition of total protein synthesis measured by valine incorporation in the perfused rat liver. Rates of protein degradation were estimated by perfusing livers prelabeled in vivo with L-[1-14C]valine with medium containing 15 mM L-valine. Thus labeled valine released from liver protein during perfusion was greatly diluted and reincorporation of label was minimized. Cycloheximide at 18 muM inhibited protein degradation by over 60%, after a delay of 15-20 min. Associated with these effects were dose-dependent increases in the rates of glucose and urea production. Glucose production increased 3 fold, from 0.54 +/- 0.07  in control to 1.85 +/- 0.24 mumol/min/100 g rat in cycloheximide-treated livers. Urea production increased from 0.24 +/- 0.02 to 0.62 +/- 0.06 mumol/min/100 g rat. No changes in liver glycogen or cyclic AMP content were seen. The data suggest that inhibition of protein synthesis provides an increased availability of intra-cellular amino acids and that many of these are rapidly degraded, yielding urea and glucose. This is supported by the fact that intracellular alanine levels were significantly increased following cycloheximide treatment. It is possible that the inhibition of protein degradation by cycloheximide is due to altered intra-cellular pools of amino acids or their metabolites."}
{"id": "PMID:1082352", "title": "Restoration of cellular immune response by levamisole in patients with Hodgkin's disease.", "content": "Cellular responses were studied in patients with Hodgkin's disease before and after levamisole treatment \"in vivo\" by measuring delayed skin reactivity to various antigens (PPD, Mumps, Candida and SK-SD), and \"in vitro\" by evaluating lymphocyte capacity to form spontaneous rosettes and to react to the T-cell mitogen PHA. Levamisole was found to significantly increase both the delayed skin reactivity and the number of T-rosette forming lymphocytes. Patients within two years from irradiation had reduced reactivity to PHA and in them levamisole significantly increased this reactivity. On the other hand, patients who had been irradiated more than two years prior to the study had normal reactivity to PHA which tended to decrease under levamisole treatment. It is concluded that levamisole restores the depressed cellular immunity in patients with Hodgkin's disease and its administration might be indicated in patients, especially in the immediate post-irradiation period.", "contents": "Restoration of cellular immune response by levamisole in patients with Hodgkin's disease. Cellular responses were studied in patients with Hodgkin's disease before and after levamisole treatment \"in vivo\" by measuring delayed skin reactivity to various antigens (PPD, Mumps, Candida and SK-SD), and \"in vitro\" by evaluating lymphocyte capacity to form spontaneous rosettes and to react to the T-cell mitogen PHA. Levamisole was found to significantly increase both the delayed skin reactivity and the number of T-rosette forming lymphocytes. Patients within two years from irradiation had reduced reactivity to PHA and in them levamisole significantly increased this reactivity. On the other hand, patients who had been irradiated more than two years prior to the study had normal reactivity to PHA which tended to decrease under levamisole treatment. It is concluded that levamisole restores the depressed cellular immunity in patients with Hodgkin's disease and its administration might be indicated in patients, especially in the immediate post-irradiation period."}
{"id": "PMID:1082355", "title": "The action of acetylcholine antagonists on amino acid responses in the frog spinal cord in vitro.", "content": "1 The isolated hemisected frog spinal cord has been used to study the action of acetylcholine antagonists on amino acid responses by means of sucrose gap recording. 2 Primary afferents and motoneurones were shown to contain few, if any, cholinoceptors, since acetylcholine and carbachol responses were essentially abolished when synaptic transmission was blocked with magnesium ions or when action potentials were blocked by tetrodotoxin. 3 Curare antagonized the gamma-aminobutyric acid (GABA) and beta-alanine depolarizations of primary afferents and hyperpolarizing action of these amino acids on motoneurones. Nicotine also antagonized beta-alanine depolarizations and to a small extent GABA depolarizations of primary afferents. These actions are similar to but weaker than those obtained previously with picrotoxin. 4 Atropine selectively antagonized beta-alanine depolarizations of primary afferents and blocked beta-alanine and glycine hyperpolarizations of motoneurones. GABA responses were entirely resistant to the action of atropine. These actions are similar to but 50 times weaker than those obtained previously with strychnine. 5 Dihydro-beta-erythroidine, tetraethylammonium, and gallamine were entirely ineffective in antagonizing amino acid responses. Since these agents are known to block the dorsal root potential elicited by ventral root stimulation but have no effect on the amino acid responses of primary afferents, it is evident that a cholinergic step is involved in this pathway.", "contents": "The action of acetylcholine antagonists on amino acid responses in the frog spinal cord in vitro. 1 The isolated hemisected frog spinal cord has been used to study the action of acetylcholine antagonists on amino acid responses by means of sucrose gap recording. 2 Primary afferents and motoneurones were shown to contain few, if any, cholinoceptors, since acetylcholine and carbachol responses were essentially abolished when synaptic transmission was blocked with magnesium ions or when action potentials were blocked by tetrodotoxin. 3 Curare antagonized the gamma-aminobutyric acid (GABA) and beta-alanine depolarizations of primary afferents and hyperpolarizing action of these amino acids on motoneurones. Nicotine also antagonized beta-alanine depolarizations and to a small extent GABA depolarizations of primary afferents. These actions are similar to but weaker than those obtained previously with picrotoxin. 4 Atropine selectively antagonized beta-alanine depolarizations of primary afferents and blocked beta-alanine and glycine hyperpolarizations of motoneurones. GABA responses were entirely resistant to the action of atropine. These actions are similar to but 50 times weaker than those obtained previously with strychnine. 5 Dihydro-beta-erythroidine, tetraethylammonium, and gallamine were entirely ineffective in antagonizing amino acid responses. Since these agents are known to block the dorsal root potential elicited by ventral root stimulation but have no effect on the amino acid responses of primary afferents, it is evident that a cholinergic step is involved in this pathway."}
{"id": "PMID:1082357", "title": "Thyroid and other autoantibodies in British and Japanese women: an epidemiological study of breast cancer.", "content": "To define the role of asymptomatic autoimmune thyroiditis in the cause of breast cancer, the presence of circulating thyroid autoantibodies was studied in two populations, one with a high risk of breast cancer (British women) and one with a low risk (Japanese women). Ostensibly healthy women and patients with breast cancer from both countries were studied. There was no difference in the incidence of thyroid autoantibodies between women with breast cancer and healthy women in either race. The incidence of thyroid autoantibodies in healthy British women, however, was two to three times that in healthy Japanese women. The incidence of reticulin antibodies, was considerably higher in both groups of Japanese women. No remarkable differences in the incidence of antinuclear, smooth-muscle, antimitochondrial, gastric parietal cell, or liver-kidney microsomal antibodies were found between women with breast cancer and healthy women or between the two races. Only the incidence of antinuclear antibodies was marginally higher in Japanese patients with advanced cancer. These results indicate that asymptomatic autoimmune thyroid disease is more prevalent among British than among Japanese women, but they fail to provide direct evidence that autoimmune thyroid disease is associated with breast cancer. Prospective studies of women with autoimmune thyroiditis and studies of young women from low-risk and high-risk populations are needed.", "contents": "Thyroid and other autoantibodies in British and Japanese women: an epidemiological study of breast cancer. To define the role of asymptomatic autoimmune thyroiditis in the cause of breast cancer, the presence of circulating thyroid autoantibodies was studied in two populations, one with a high risk of breast cancer (British women) and one with a low risk (Japanese women). Ostensibly healthy women and patients with breast cancer from both countries were studied. There was no difference in the incidence of thyroid autoantibodies between women with breast cancer and healthy women in either race. The incidence of thyroid autoantibodies in healthy British women, however, was two to three times that in healthy Japanese women. The incidence of reticulin antibodies, was considerably higher in both groups of Japanese women. No remarkable differences in the incidence of antinuclear, smooth-muscle, antimitochondrial, gastric parietal cell, or liver-kidney microsomal antibodies were found between women with breast cancer and healthy women or between the two races. Only the incidence of antinuclear antibodies was marginally higher in Japanese patients with advanced cancer. These results indicate that asymptomatic autoimmune thyroid disease is more prevalent among British than among Japanese women, but they fail to provide direct evidence that autoimmune thyroid disease is associated with breast cancer. Prospective studies of women with autoimmune thyroiditis and studies of young women from low-risk and high-risk populations are needed."}
{"id": "PMID:1082358", "title": "Radiology and endoscopy in acute upper gastrointestinal bleeding.", "content": "Of 112 patients admitted with acute upper gastrointestinal bleeding, the presumed bleeding site was detected in 61-5% of cases by radiology and in 57% of cases on endoscopy. Thirty-one patients who had barium-meal examination were operated on and the surgical and radiological findings agreed in 26 (84%). Twenty-three patients who had endoscopy were operated on and the surgical and endoscopic findings agreed in 15 (65%). In 10 cases radiology detected a lesion not identified on endoscopy and in nine endoscopy detected a lesion not seen at radiology. We suggest that when there are two potential sources of bleeding radiology as well as endoscopy can detect the actively bleeding lesion. The supplementary nature of radiology and endoscopy is emphasised and we conclude that both methods should be used if there is any doubt at the initial radiological or endoscopic examination about the source of the bleeding.", "contents": "Radiology and endoscopy in acute upper gastrointestinal bleeding. Of 112 patients admitted with acute upper gastrointestinal bleeding, the presumed bleeding site was detected in 61-5% of cases by radiology and in 57% of cases on endoscopy. Thirty-one patients who had barium-meal examination were operated on and the surgical and radiological findings agreed in 26 (84%). Twenty-three patients who had endoscopy were operated on and the surgical and endoscopic findings agreed in 15 (65%). In 10 cases radiology detected a lesion not identified on endoscopy and in nine endoscopy detected a lesion not seen at radiology. We suggest that when there are two potential sources of bleeding radiology as well as endoscopy can detect the actively bleeding lesion. The supplementary nature of radiology and endoscopy is emphasised and we conclude that both methods should be used if there is any doubt at the initial radiological or endoscopic examination about the source of the bleeding."}
{"id": "PMID:1082361", "title": "Efferent projections of the superior olivary nucleus in the frog, Rana catesbeiana.", "content": "Following electrolytic lesions of the superior olivary nucleus (SO) of the bullfrog, Rana catesbeiana, the efferent pathways of this nucleus were studied with silver impregnation methods. The major projection was to the ipsilateral principal nucleus of the torus semicircularis. Less conspicuous degeneration was traced to the nuclei magnocellularis and commissuralis of the torus. No contribution was made to the tectum or nucleus isthmi. Other projections of the SO include a sma-ler contralateral projection to these same nuclei and to the ipsilateral nucleus lateralis profundus mesencephali. A system of connections between the acoustic nuclei was invariably interrupted with these lesions and the degeneration seen in the more dorsal acoustic areas was not entirely due to the destruction of SO neurons.", "contents": "Efferent projections of the superior olivary nucleus in the frog, Rana catesbeiana. Following electrolytic lesions of the superior olivary nucleus (SO) of the bullfrog, Rana catesbeiana, the efferent pathways of this nucleus were studied with silver impregnation methods. The major projection was to the ipsilateral principal nucleus of the torus semicircularis. Less conspicuous degeneration was traced to the nuclei magnocellularis and commissuralis of the torus. No contribution was made to the tectum or nucleus isthmi. Other projections of the SO include a sma-ler contralateral projection to these same nuclei and to the ipsilateral nucleus lateralis profundus mesencephali. A system of connections between the acoustic nuclei was invariably interrupted with these lesions and the degeneration seen in the more dorsal acoustic areas was not entirely due to the destruction of SO neurons."}
{"id": "PMID:1082363", "title": "The risk of iridocyclitis in juvenile rheumatoid arthritis.", "content": "A retrospective study comparing 14 patients with juvenile rheumatoid arthritis (JRA) complicated by chronic iridocyclitis (Group I) with an equal number of JRA patients without this complication (Group II) was conducted to determine which factors might aid in the earlier diagnosis of chronic iridocyclitis. Eleven patients in Group I had oligoarticular disease compared to 5 in Group II. Antinuclear antibody was found in 13 patients in Group I compared to only 2 in Group II. Only one of nine patients tested in Group I for the histocompatibility antigen W-27 was positive. The patient with oligoarticular disease and a positive ANA test should be followed closely by the ophthalmologist for the development of chronic iridocyclitis.", "contents": "The risk of iridocyclitis in juvenile rheumatoid arthritis. A retrospective study comparing 14 patients with juvenile rheumatoid arthritis (JRA) complicated by chronic iridocyclitis (Group I) with an equal number of JRA patients without this complication (Group II) was conducted to determine which factors might aid in the earlier diagnosis of chronic iridocyclitis. Eleven patients in Group I had oligoarticular disease compared to 5 in Group II. Antinuclear antibody was found in 13 patients in Group I compared to only 2 in Group II. Only one of nine patients tested in Group I for the histocompatibility antigen W-27 was positive. The patient with oligoarticular disease and a positive ANA test should be followed closely by the ophthalmologist for the development of chronic iridocyclitis."}
{"id": "PMID:1082364", "title": "Chemotherapy, en bloc resection, and prosthetic bone replacement in the treatment of osteogenic sarcoma.", "content": "In an attempt to shrink primary osteogenic sarcoma and allow complete surgical removal of the primary tumor, without amputating the involved limb, intensive preoperative chemotherapy with high dose methotrexate (HDMTX) with citrovorum factor rescue (CFR) and adriamycin (ADR) was initiated in 20 patients with biopsy-proven primary osteogenic sarcoma of the distal femur (15 patients) and proximal tibia (five patients). Following intensive chemotherapy, en bloc resection of the primary tumor with prosthetic replacement of the involved bone was planned. After surgery, adjuvant chemotherapy, consisting of HDMTX with CFR, ADR, and high dose cyclophosphamide was given sequentially for 1 year. Of 20 patients with primary osteogenic sarcoma (two with evidence of pulmonary metastases), 18 had primary tumors that could be clinically measured. Of these 18, 17 demonstrated a decrease in the size of primary tumor prior to surgery, while on chemotherapy. To date, 12 of these patients with osteogenic sarcoma of the distal femur have had total femur and knee joint replacement, and three patients with osteogenic sarcoma of the proximal tibia have had total knee replacement. In all 15 patients, surgical margins were grossly and microscopically free of tumor. There has been no evidence of soft tissue recurrence in any of the 15 patients who have undergone surgery for from 2 to 15 months postoperatively. These preliminary results indicate that with the use of aggressive chemotherapy, it is possible to demonstrate objective tumor regression in primary osteogenic sarcoma, allowing the surgeon to perform en bloc resection of tumor and prosthetic replacement of the involved bone. Although the limb is preserved, it is important to stress that extensive surgery yielding tumor-free margins is performed. The ultimate evaluation of this approach to the treatment of primary osteogenic sarcoma awaits longer observation, to determine limb function and the continued disease-free status, once adjuvant chemotherapy is discontinued.", "contents": "Chemotherapy, en bloc resection, and prosthetic bone replacement in the treatment of osteogenic sarcoma. In an attempt to shrink primary osteogenic sarcoma and allow complete surgical removal of the primary tumor, without amputating the involved limb, intensive preoperative chemotherapy with high dose methotrexate (HDMTX) with citrovorum factor rescue (CFR) and adriamycin (ADR) was initiated in 20 patients with biopsy-proven primary osteogenic sarcoma of the distal femur (15 patients) and proximal tibia (five patients). Following intensive chemotherapy, en bloc resection of the primary tumor with prosthetic replacement of the involved bone was planned. After surgery, adjuvant chemotherapy, consisting of HDMTX with CFR, ADR, and high dose cyclophosphamide was given sequentially for 1 year. Of 20 patients with primary osteogenic sarcoma (two with evidence of pulmonary metastases), 18 had primary tumors that could be clinically measured. Of these 18, 17 demonstrated a decrease in the size of primary tumor prior to surgery, while on chemotherapy. To date, 12 of these patients with osteogenic sarcoma of the distal femur have had total femur and knee joint replacement, and three patients with osteogenic sarcoma of the proximal tibia have had total knee replacement. In all 15 patients, surgical margins were grossly and microscopically free of tumor. There has been no evidence of soft tissue recurrence in any of the 15 patients who have undergone surgery for from 2 to 15 months postoperatively. These preliminary results indicate that with the use of aggressive chemotherapy, it is possible to demonstrate objective tumor regression in primary osteogenic sarcoma, allowing the surgeon to perform en bloc resection of tumor and prosthetic replacement of the involved bone. Although the limb is preserved, it is important to stress that extensive surgery yielding tumor-free margins is performed. The ultimate evaluation of this approach to the treatment of primary osteogenic sarcoma awaits longer observation, to determine limb function and the continued disease-free status, once adjuvant chemotherapy is discontinued."}
{"id": "PMID:1082365", "title": "An attempt at synchronization of marrow cells in acute leukemia: relationship to therapeutic response.", "content": "The relationship between changes in the bone marrow labeling index and the patient's subsequent response to cycle-specific agents was studied by the South-eastern Cancer Study Group in adults with acute leukemia. Ninety-eight patients were randomized to one of two treatment regimens. Schedule 1 consisted of a single intravenous (i.v.) push of cytosine arabinoside followed in 48 hours by a large dose of oral methotrexate distributed over 24 hours and i.v. vincristine. Leucovorin rescue was employed to control the toxic effects of the high dose methotrexate and the cycle was repeated every 7 days. Schedule 2 differed only in that there were three daily injections of cytosine arabinoside preceding vincristine and methotrexate injections and each cycle was given every 10 days. Cell kinetic studies were performed in 30 patients and revealed that the majority of patients who had a response to therapy had some increase in the marrow labeling index 48 hours after cytosine arabinoside injection. In general, those patients who had no response to therapy had little change. There was no significant difference between schedules in the ability to induce an increase in labeling index 48 hours after cytosine arabinoside or in the increment achieved by the responders. However, there was a significant difference in the response rate seen with these schedules. Schedule 1 achieved only a 24% remission rate in acute nonlymphocytic leukemia (ANLL) while schedule 2 was associated with a 52% remission rate. In acute lymphoblastic leukemia (ALL) both schedules induced a 60% remission rate while none of the four patients with blast crisis of chronic granulocytic leukemia (CGL) responded. Analysis of the characteristics associated with remission revealed that more females achieved a remission than males and that the presence of pretreatment infection was the greatest contributing cause of early death and thus severely limited the ability to achieve a remission. As opposed to the current regimens used in ANLL, schedule 2 did not require significant bone marrow hypoplasia (as judged by the degree of hematological toxicity) to achieve a remission and there was no decrease in response seen with increasing age. The data suggest that increased efficiency of cycle-specific, antitumor agents may occur by increasing the proportion of human leukemic cells in DNA synthesis.", "contents": "An attempt at synchronization of marrow cells in acute leukemia: relationship to therapeutic response. The relationship between changes in the bone marrow labeling index and the patient's subsequent response to cycle-specific agents was studied by the South-eastern Cancer Study Group in adults with acute leukemia. Ninety-eight patients were randomized to one of two treatment regimens. Schedule 1 consisted of a single intravenous (i.v.) push of cytosine arabinoside followed in 48 hours by a large dose of oral methotrexate distributed over 24 hours and i.v. vincristine. Leucovorin rescue was employed to control the toxic effects of the high dose methotrexate and the cycle was repeated every 7 days. Schedule 2 differed only in that there were three daily injections of cytosine arabinoside preceding vincristine and methotrexate injections and each cycle was given every 10 days. Cell kinetic studies were performed in 30 patients and revealed that the majority of patients who had a response to therapy had some increase in the marrow labeling index 48 hours after cytosine arabinoside injection. In general, those patients who had no response to therapy had little change. There was no significant difference between schedules in the ability to induce an increase in labeling index 48 hours after cytosine arabinoside or in the increment achieved by the responders. However, there was a significant difference in the response rate seen with these schedules. Schedule 1 achieved only a 24% remission rate in acute nonlymphocytic leukemia (ANLL) while schedule 2 was associated with a 52% remission rate. In acute lymphoblastic leukemia (ALL) both schedules induced a 60% remission rate while none of the four patients with blast crisis of chronic granulocytic leukemia (CGL) responded. Analysis of the characteristics associated with remission revealed that more females achieved a remission than males and that the presence of pretreatment infection was the greatest contributing cause of early death and thus severely limited the ability to achieve a remission. As opposed to the current regimens used in ANLL, schedule 2 did not require significant bone marrow hypoplasia (as judged by the degree of hematological toxicity) to achieve a remission and there was no decrease in response seen with increasing age. The data suggest that increased efficiency of cycle-specific, antitumor agents may occur by increasing the proportion of human leukemic cells in DNA synthesis."}
{"id": "PMID:1082366", "title": "The effect of thymosin on human T-cells from cancer patients.", "content": "The effect of thymosin on in vitro reactivity of peripheral lymphocytes to phytohemagglutinin, concanavalin A, and the formation of spontaneous E-rosettes in 54 patients with metastatic carcinomas has been studied. Thymosin increased lymphocyte responses to PHA and Con A in only 10 patients, with predominant effect seen with Con A. Twenty patients showed depressed baseline levels of E-rosettes which were increased to normal or subnormal levels after incubation with thymosin. No distinct correlation was noted between the clinical stage of the disease and the ability of lymphocytes to form E-rosettes. Although the exact mechanism by which the thymus exerts its influence on host resistance is not clearly defined, present evidence supports the concept that the thymic hormone, thymosin, may be an important addition in treatment of cancer patients by increasing cell-mediated immunity.", "contents": "The effect of thymosin on human T-cells from cancer patients. The effect of thymosin on in vitro reactivity of peripheral lymphocytes to phytohemagglutinin, concanavalin A, and the formation of spontaneous E-rosettes in 54 patients with metastatic carcinomas has been studied. Thymosin increased lymphocyte responses to PHA and Con A in only 10 patients, with predominant effect seen with Con A. Twenty patients showed depressed baseline levels of E-rosettes which were increased to normal or subnormal levels after incubation with thymosin. No distinct correlation was noted between the clinical stage of the disease and the ability of lymphocytes to form E-rosettes. Although the exact mechanism by which the thymus exerts its influence on host resistance is not clearly defined, present evidence supports the concept that the thymic hormone, thymosin, may be an important addition in treatment of cancer patients by increasing cell-mediated immunity."}
{"id": "PMID:1082367", "title": "The role of upper gastrointestinal endoscopy in patients with cancer.", "content": "The last decade has seen considerable advance in the application of fiberoptic endoscopy to the diagnosis and management of patients with cancer. Forward and side-viewing instruments have been developed that have complete tip control, potential for obtaining directed biopsies, brush and lavage cytology, and for aspiration of material for assay of enzymes and tumor-associated antigens. These features have provided a high degree of diagnostic capability in the esophagus, stomach, and duodenum. Accurate histologic diagnoses require multiple biopsies and brush cytology from each lesion. Lavage cytology has been useful in selected patients, especially those with diffusely infiltrating lesions. Brush cytology has been especially helpful in patients with stenotic esophageal cancer. These techniques have also provided a diagnosis in a high percentage of patients with lymphoma involving the stomach. Endoscopy has also been very useful in patients with nongastrointestinal cancer who have major gastrointestinal problems such as bleeding stress ulcers or monilial esophagitis.", "contents": "The role of upper gastrointestinal endoscopy in patients with cancer. The last decade has seen considerable advance in the application of fiberoptic endoscopy to the diagnosis and management of patients with cancer. Forward and side-viewing instruments have been developed that have complete tip control, potential for obtaining directed biopsies, brush and lavage cytology, and for aspiration of material for assay of enzymes and tumor-associated antigens. These features have provided a high degree of diagnostic capability in the esophagus, stomach, and duodenum. Accurate histologic diagnoses require multiple biopsies and brush cytology from each lesion. Lavage cytology has been useful in selected patients, especially those with diffusely infiltrating lesions. Brush cytology has been especially helpful in patients with stenotic esophageal cancer. These techniques have also provided a diagnosis in a high percentage of patients with lymphoma involving the stomach. Endoscopy has also been very useful in patients with nongastrointestinal cancer who have major gastrointestinal problems such as bleeding stress ulcers or monilial esophagitis."}
{"id": "PMID:1082368", "title": "Granulocytic stem cells in Friend leukemia.", "content": "Friend virus induces a leukemia characterized by the proliferation of neoplastic hematopoietic cells believed to be erythroid precursors. In vitro studies were conducted with spleen cells from mice with terminal Firend leukemia in order to determine their capacity for leukocytic differentiation. Spleen cells were obtained from leukemic DBA/2 mice 1 to 2 days before anticipated death and cultured in the presence or absence of colony-stimulating activity (CSA). Growth in liquid culture in dissusion chambers was dependent on CSA and resulted in the generation of normally differentiated granulocytes and macrophages. Colony formation in agar was also dependent on CSA, and the cloning efficiency of leukemic spleen cells was found to be approximately 10 times normal. The colonies formed were composed of leukocytes, which appeared morphologically normal. Total in vitro colony-forming units per leukemic spleen exceeded normal by more than 300-fold, but cells elaborating CSA were decreased. Although it is uncertain whether the stem cells stimulated by CSA are \"normal\" or leukemic,\" it is clear that Friend leukemia has profound effects on the proliferation and differentiation of nonerythroid stem cells.", "contents": "Granulocytic stem cells in Friend leukemia. Friend virus induces a leukemia characterized by the proliferation of neoplastic hematopoietic cells believed to be erythroid precursors. In vitro studies were conducted with spleen cells from mice with terminal Firend leukemia in order to determine their capacity for leukocytic differentiation. Spleen cells were obtained from leukemic DBA/2 mice 1 to 2 days before anticipated death and cultured in the presence or absence of colony-stimulating activity (CSA). Growth in liquid culture in dissusion chambers was dependent on CSA and resulted in the generation of normally differentiated granulocytes and macrophages. Colony formation in agar was also dependent on CSA, and the cloning efficiency of leukemic spleen cells was found to be approximately 10 times normal. The colonies formed were composed of leukocytes, which appeared morphologically normal. Total in vitro colony-forming units per leukemic spleen exceeded normal by more than 300-fold, but cells elaborating CSA were decreased. Although it is uncertain whether the stem cells stimulated by CSA are \"normal\" or leukemic,\" it is clear that Friend leukemia has profound effects on the proliferation and differentiation of nonerythroid stem cells."}
{"id": "PMID:1082369", "title": "Critical evaluation of lymphocyte functions in urological cancer patients.", "content": "One hundred three patients with varying stages of urological cancer (bladder, prostate, kidney) were investigated with regard to the following lymphocyte functions. T-cells were assessed numerically (E rosettes), their blastogenic response to phytohemagglutinin (pHA) was determined, and their cytotoxic potential against heterologous target cells in short-term presence of PHA (e.i., PHA-dependent cellular cytotoxocity) was evaluated. Similarly, B cells were numerically assessed (EA rosettes), and their function was evaluated by antibody-dependent cellular cytotoxicity against antibody-coated deterologous target cells. The data on cancer patients, divided on the basis of extent of disease and prior radiation therapy, were compared to those of normal young and age-matched controls. Our investigations emphasize the importance of the following factors: (a) comparison of data with age-matched controls, since several lymphocyte functions appear to change with age; (b) use of multiple controls to compensate for the inherent variability found in certain tests; (c) minimized contamination by nonlymphoid cells in the purified cell preparation; and (d) the influence of certain treatment regimens (radiation, chemotherapy, etc.) on the results. Radiotherapy significantly depressed T-cell number with a depression of PHA blastogenic responses as well as PHA-dependent cellular cytotoxicity. When all of these conditions were taken into account, the urological cancer patients as a group were found to have a lower proportional value of E rosettes (T-cells) and a reduced PHA blastogenic responsiveness. Certain cancer patients displayed an elevated PHA-dependent cellular cytotoxicity as compared to age-matched controls, which may indicate the presence of activated cells in the presence of tumors. With this identification of a group of cancer patients with markedly depressed E rosette values and PHA responsiveness, it will now be possible to follow them clinically in comparison with a group of cancer patients with normal T-cell functions.", "contents": "Critical evaluation of lymphocyte functions in urological cancer patients. One hundred three patients with varying stages of urological cancer (bladder, prostate, kidney) were investigated with regard to the following lymphocyte functions. T-cells were assessed numerically (E rosettes), their blastogenic response to phytohemagglutinin (pHA) was determined, and their cytotoxic potential against heterologous target cells in short-term presence of PHA (e.i., PHA-dependent cellular cytotoxocity) was evaluated. Similarly, B cells were numerically assessed (EA rosettes), and their function was evaluated by antibody-dependent cellular cytotoxicity against antibody-coated deterologous target cells. The data on cancer patients, divided on the basis of extent of disease and prior radiation therapy, were compared to those of normal young and age-matched controls. Our investigations emphasize the importance of the following factors: (a) comparison of data with age-matched controls, since several lymphocyte functions appear to change with age; (b) use of multiple controls to compensate for the inherent variability found in certain tests; (c) minimized contamination by nonlymphoid cells in the purified cell preparation; and (d) the influence of certain treatment regimens (radiation, chemotherapy, etc.) on the results. Radiotherapy significantly depressed T-cell number with a depression of PHA blastogenic responses as well as PHA-dependent cellular cytotoxicity. When all of these conditions were taken into account, the urological cancer patients as a group were found to have a lower proportional value of E rosettes (T-cells) and a reduced PHA blastogenic responsiveness. Certain cancer patients displayed an elevated PHA-dependent cellular cytotoxicity as compared to age-matched controls, which may indicate the presence of activated cells in the presence of tumors. With this identification of a group of cancer patients with markedly depressed E rosette values and PHA responsiveness, it will now be possible to follow them clinically in comparison with a group of cancer patients with normal T-cell functions."}
{"id": "PMID:1082370", "title": "Stimulatory effects of 5-lodo-2'-deoxyuridine on number and function of splenic B- and T-cells and of macrophages in C3HeB/FeJ mice.", "content": "5-lodo-2'-deoxyuridine (lUdR) significantly increases the total number of spleen cells on the fourth day after administration of 100 mg/kg in normal C3HeB/FeJ male mice. The number of splenic B-cells [cells sensitive to the cytotoxic activity of a rabbit antiserum to mouse F(ab)2, plus complement] increases on the same day. The number of T-cells (cells sensitive to the cytotoxic activity of a rabbit antiserum to mouse brain-associated theta antigen, plus complement) and of macrophages (adherent-phagocytic cells) does not increase. Spleen cells from lUdR-treated mice show heightened responsiveness in vitro to Escherichia coli lipopolysaccharide or concanavalin A on Day 4 and Days 5 to 6, respectively. The rate of clearance of i.v.-injected colloidal carbon is also increased for 3 days after lUdR. Thus, lUdR is able to functionally activate either T- or B-cells or macrophages, but only to increase the number of splenic B-cells.", "contents": "Stimulatory effects of 5-lodo-2'-deoxyuridine on number and function of splenic B- and T-cells and of macrophages in C3HeB/FeJ mice. 5-lodo-2'-deoxyuridine (lUdR) significantly increases the total number of spleen cells on the fourth day after administration of 100 mg/kg in normal C3HeB/FeJ male mice. The number of splenic B-cells [cells sensitive to the cytotoxic activity of a rabbit antiserum to mouse F(ab)2, plus complement] increases on the same day. The number of T-cells (cells sensitive to the cytotoxic activity of a rabbit antiserum to mouse brain-associated theta antigen, plus complement) and of macrophages (adherent-phagocytic cells) does not increase. Spleen cells from lUdR-treated mice show heightened responsiveness in vitro to Escherichia coli lipopolysaccharide or concanavalin A on Day 4 and Days 5 to 6, respectively. The rate of clearance of i.v.-injected colloidal carbon is also increased for 3 days after lUdR. Thus, lUdR is able to functionally activate either T- or B-cells or macrophages, but only to increase the number of splenic B-cells."}
{"id": "PMID:1082371", "title": "The ultrastructure of taste and touch receptors of the frog's taste organ.", "content": "The taste buds from fungiform papillae and the hard palate of frogs were investigated with the scanning and transmission electron microscopes. An immature pre-taste cell and a mature taste cell can be differentiated. Only the mature taste cell exhibits synaptic contact with the afferent taste fibre. Glandular and satellite supporting cells envelop the thin apical processes of the sensory cells. At the base of the taste disc up to 10 Merkel cells form a complex with nerve endings. There are two types of myelinated fibres, large and small. The small fibre innervates the taste cells, the thicker nerve fibre the Merkel cells. The occurrence of two types of receptors explains physiological results.", "contents": "The ultrastructure of taste and touch receptors of the frog's taste organ. The taste buds from fungiform papillae and the hard palate of frogs were investigated with the scanning and transmission electron microscopes. An immature pre-taste cell and a mature taste cell can be differentiated. Only the mature taste cell exhibits synaptic contact with the afferent taste fibre. Glandular and satellite supporting cells envelop the thin apical processes of the sensory cells. At the base of the taste disc up to 10 Merkel cells form a complex with nerve endings. There are two types of myelinated fibres, large and small. The small fibre innervates the taste cells, the thicker nerve fibre the Merkel cells. The occurrence of two types of receptors explains physiological results."}
{"id": "PMID:1082372", "title": "The fine structure of the perineural endothelium.", "content": "Fine strands of motor nerves were examined with the electron microscope using thin section as well as freeze-etching techniques. The specimens were taken from frog cutaneous pectoris nerve, rat sciatic nerve, mouse and shrew phrenic nerves and from human skin nerves. The perineural sheath (Henle, Ranvier, Key and Retzius) consists of one to several concentric laminae of endothelial cells; it encases nerve fascicles and eventually individual nerve fibers and terminals. The endothelial cells are extremely thin and fitted togeether smoothly by overlap and dove-tailing of their border zones. The cell contacts are formed by continuous zonulae occludentes, often reinforced by maculae adhaerentes, and in depth they comprise 3-15 strands with an average of 5-6 strands per junction. The membranes of endothelial cells are studded with attachment sites and stomata of plasmalemmal vesicles suggesting a high level of pinocytotic activity. This phenomenon is by no means restricted to the external laminae of the endothelial sheath. Each endothelial lamina is vested with basement membranes on both (epineural and endoneural) sides, and the spaces between laminae contain a few collagen fibers and fibroblasts. Occasionally, punctate tight junctions are seen between laminae. Cytological evidence supports the hypothesis that the perineural endothelium provides a relatively tight and highly selective barrier separating the peripheral nerves from surrounding tissue and its extracellular fluid spaces. This effect is achieved on the one hand by the sealing of pericellular spaces and on the other hand by a membrane controlled transcellular transport mechanism (pinocytosis), both of which are enhanced by their serial arrangement.", "contents": "The fine structure of the perineural endothelium. Fine strands of motor nerves were examined with the electron microscope using thin section as well as freeze-etching techniques. The specimens were taken from frog cutaneous pectoris nerve, rat sciatic nerve, mouse and shrew phrenic nerves and from human skin nerves. The perineural sheath (Henle, Ranvier, Key and Retzius) consists of one to several concentric laminae of endothelial cells; it encases nerve fascicles and eventually individual nerve fibers and terminals. The endothelial cells are extremely thin and fitted togeether smoothly by overlap and dove-tailing of their border zones. The cell contacts are formed by continuous zonulae occludentes, often reinforced by maculae adhaerentes, and in depth they comprise 3-15 strands with an average of 5-6 strands per junction. The membranes of endothelial cells are studded with attachment sites and stomata of plasmalemmal vesicles suggesting a high level of pinocytotic activity. This phenomenon is by no means restricted to the external laminae of the endothelial sheath. Each endothelial lamina is vested with basement membranes on both (epineural and endoneural) sides, and the spaces between laminae contain a few collagen fibers and fibroblasts. Occasionally, punctate tight junctions are seen between laminae. Cytological evidence supports the hypothesis that the perineural endothelium provides a relatively tight and highly selective barrier separating the peripheral nerves from surrounding tissue and its extracellular fluid spaces. This effect is achieved on the one hand by the sealing of pericellular spaces and on the other hand by a membrane controlled transcellular transport mechanism (pinocytosis), both of which are enhanced by their serial arrangement."}
{"id": "PMID:1082375", "title": "[The so-called immunologic classification of non-Hodgkin's malignant lymphoma].", "content": "The so-called immunologic classification of malignant lymphomas was used in 100 of suitable cases. Without the simultaneous use of immunocytological methods the classification was  applicable with certain restrictions only, but, in general the entire classification scheme could be used in routine bioptic practice. The so-called immunologic classification permits a more accurate determination of the nature of the neoplasms and a larger flexibility in classifying them considering the possible trend of alterations that may occur during the course of the disease. Some difficulties and points of confusion have been discussed.", "contents": "[The so-called immunologic classification of non-Hodgkin's malignant lymphoma]. The so-called immunologic classification of malignant lymphomas was used in 100 of suitable cases. Without the simultaneous use of immunocytological methods the classification was  applicable with certain restrictions only, but, in general the entire classification scheme could be used in routine bioptic practice. The so-called immunologic classification permits a more accurate determination of the nature of the neoplasms and a larger flexibility in classifying them considering the possible trend of alterations that may occur during the course of the disease. Some difficulties and points of confusion have been discussed."}
{"id": "PMID:1082376", "title": "[Primary pulmonary histiocytosis X].", "content": "The diagnosis of a pulmonary form of histiocytosis X was made on the basis of a lung tissue biopsy in a male aged 60 suffering from progressive dyspnoe of respiratory type and a rentgenological finding of tiny disseminated foci in both lung fields. The bad prognosis of the process related to the fibrous transformation of neoplastic foci rather than to the biological nature of the process as such.", "contents": "[Primary pulmonary histiocytosis X]. The diagnosis of a pulmonary form of histiocytosis X was made on the basis of a lung tissue biopsy in a male aged 60 suffering from progressive dyspnoe of respiratory type and a rentgenological finding of tiny disseminated foci in both lung fields. The bad prognosis of the process related to the fibrous transformation of neoplastic foci rather than to the biological nature of the process as such."}
{"id": "PMID:1082378", "title": "Individual household water supplies as a control measure against Schistosoma mansoni. A study in rural St Lucia.", "content": "As part of a programme to evaluate single control measures for reducing the transmission of Schistosoma mansoni, household water supplies were installed in 5 rural settlements in the Riche Fond Valley of St Lucia. About 2 000 persons who previously were dependent on rivers and streams are now receiving safe water at their homes. The systems provide useful design data on individual water requirements in rural areas. This experience suggests that future rural water systems can be designed more economically and efficiently by using consumption rates that are closer to the actual requirements and by eliminating water wastage at the taps.", "contents": "Individual household water supplies as a control measure against Schistosoma mansoni. A study in rural St Lucia. As part of a programme to evaluate single control measures for reducing the transmission of Schistosoma mansoni, household water supplies were installed in 5 rural settlements in the Riche Fond Valley of St Lucia. About 2 000 persons who previously were dependent on rivers and streams are now receiving safe water at their homes. The systems provide useful design data on individual water requirements in rural areas. This experience suggests that future rural water systems can be designed more economically and efficiently by using consumption rates that are closer to the actual requirements and by eliminating water wastage at the taps."}
{"id": "PMID:1082379", "title": "Abnormalities in the microsomal oxidases of the WHO standard reference strain of Musca domestica.", "content": "Observations made during biochemical and toxicological studies of the housefly, in which the WHO standard reference (SR) strain was used as a standard, indicated that this strain differs from other strains in certain physiological and toxicological aspects. Experiments are described in which microsomal oxidases prepared from the standard reference strain and several other housefly strains, both susceptible and resistant to insecticides, were compared for heat stability, sensitivity to ionic strength, pH optima, and the spectral characteristics of their cytochrome P-450. In a comparison with 8 strains the SR strain microsomes were more stable at high incubation temperatures (40-47.5 degrees C). Compared to two other strains (NAIDM, susceptible, and Isolan-B, resistant) SR was more stable at low pH and more sensitive to high ionic strength. In spectral comparisons with NAIDM and Isolan-B, SR was found to contain microsomal cytochrome P-450 that differed slightly in its carbon monoxide or octylamine binding spectra. The results suggest that this strain may not provide a suitable reference in studies involving the microsomal oxidases. However, these apparent differences should not reduce the value of the SR stain as a reference in insecticide resistance studies.", "contents": "Abnormalities in the microsomal oxidases of the WHO standard reference strain of Musca domestica. Observations made during biochemical and toxicological studies of the housefly, in which the WHO standard reference (SR) strain was used as a standard, indicated that this strain differs from other strains in certain physiological and toxicological aspects. Experiments are described in which microsomal oxidases prepared from the standard reference strain and several other housefly strains, both susceptible and resistant to insecticides, were compared for heat stability, sensitivity to ionic strength, pH optima, and the spectral characteristics of their cytochrome P-450. In a comparison with 8 strains the SR strain microsomes were more stable at high incubation temperatures (40-47.5 degrees C). Compared to two other strains (NAIDM, susceptible, and Isolan-B, resistant) SR was more stable at low pH and more sensitive to high ionic strength. In spectral comparisons with NAIDM and Isolan-B, SR was found to contain microsomal cytochrome P-450 that differed slightly in its carbon monoxide or octylamine binding spectra. The results suggest that this strain may not provide a suitable reference in studies involving the microsomal oxidases. However, these apparent differences should not reduce the value of the SR stain as a reference in insecticide resistance studies."}
{"id": "PMID:1082380", "title": "Field application to malaria studies of the passive haemagglutination (PHA) test with lyophilized cells.", "content": "Lyophilized cells for the passive (formerly indirect) haemagglutination test for malaria were tested in a small field trial. A comparison was made with results obtained with samples from the same persons by another method in another laboratory (Center for Disease Control, Atlanta, GA, USA). The use of lyophilized cells of standardized reactivity clearly adds to the simplicity and rapidity of the test, and justifies its recommendation for use in field studies.", "contents": "Field application to malaria studies of the passive haemagglutination (PHA) test with lyophilized cells. Lyophilized cells for the passive (formerly indirect) haemagglutination test for malaria were tested in a small field trial. A comparison was made with results obtained with samples from the same persons by another method in another laboratory (Center for Disease Control, Atlanta, GA, USA). The use of lyophilized cells of standardized reactivity clearly adds to the simplicity and rapidity of the test, and justifies its recommendation for use in field studies."}
{"id": "PMID:1082381", "title": "Single-radial-hemolysis: a new method for the assay of antibody to influenza haemagglutinin. Applications for diagnosis and seroepidemiologic surveillance of influenza.", "content": "A simple single-radial-haemolysis technique is described that permits the detection and assay of antibody to influenza virus haemagglutinin. The method depends on the passive haemolysis of virus-treated erythrocytes by antihaemagglutinin antibody and complement. Under the \"standard\" test conditions described antibody to the other surface antigen of the influenza virus (neuraminidase) or to the internal antigens of the virus (nucleoprotein and matrix protein) do not produce haemolysis. Because it requires only small amounts of crude virus antigen and is rapid and simple, the method appears to be of considerable value for large-scale seroepidemiologic studies of new influenza virus variants. Antihaemagglutinin antibody detected by single-radial-haemolysis appears to be relatively strain-specific; the technique may therefore be useful in the antigenic characterization of virus isolates.", "contents": "Single-radial-hemolysis: a new method for the assay of antibody to influenza haemagglutinin. Applications for diagnosis and seroepidemiologic surveillance of influenza. A simple single-radial-haemolysis technique is described that permits the detection and assay of antibody to influenza virus haemagglutinin. The method depends on the passive haemolysis of virus-treated erythrocytes by antihaemagglutinin antibody and complement. Under the \"standard\" test conditions described antibody to the other surface antigen of the influenza virus (neuraminidase) or to the internal antigens of the virus (nucleoprotein and matrix protein) do not produce haemolysis. Because it requires only small amounts of crude virus antigen and is rapid and simple, the method appears to be of considerable value for large-scale seroepidemiologic studies of new influenza virus variants. Antihaemagglutinin antibody detected by single-radial-haemolysis appears to be relatively strain-specific; the technique may therefore be useful in the antigenic characterization of virus isolates."}
{"id": "PMID:1082382", "title": "Experimental study of the role of inactivated vaccine in two-step vaccination against smallpox.", "content": "In experiments on rabbits it was found that although administration of inactivated smallpox vaccine did not induce a demonstrable antibody response in the serum it enhanced the immune response to subsequent inoculation with live vaccine. The dose of inactivated vaccine corresponded to 8 x 10(7) PFU before inactivation (by (60)Co gamma-radiation); the dose of live vaccine was 1.2 x 10(5) PFU. When the interval between the two inoculations was 7-days, virus-neutralizing antibody appeared after 5 days and reached levels 2-4 times those obtained with live vaccine alone. With longer intervals (up to 60 days) the enhancement of the immune response was even greater. It seems likely that use of the two-step method may reduce the incidence of post-vaccination encephalitis and clinical studies to determine the optimum conditions for safety and efficacy are at present being undertaken.", "contents": "Experimental study of the role of inactivated vaccine in two-step vaccination against smallpox. In experiments on rabbits it was found that although administration of inactivated smallpox vaccine did not induce a demonstrable antibody response in the serum it enhanced the immune response to subsequent inoculation with live vaccine. The dose of inactivated vaccine corresponded to 8 x 10(7) PFU before inactivation (by (60)Co gamma-radiation); the dose of live vaccine was 1.2 x 10(5) PFU. When the interval between the two inoculations was 7-days, virus-neutralizing antibody appeared after 5 days and reached levels 2-4 times those obtained with live vaccine alone. With longer intervals (up to 60 days) the enhancement of the immune response was even greater. It seems likely that use of the two-step method may reduce the incidence of post-vaccination encephalitis and clinical studies to determine the optimum conditions for safety and efficacy are at present being undertaken."}
{"id": "PMID:1082383", "title": "Controlled field trial of a typhoid vaccine prepared with a nonmotile mutant of Salmonella typhi Ty2.", "content": "A controlled field trial was performed in Egypt to evaluate a whole cell typhoid vaccine prepared with a nonmotile mutant of S. typhi Ty2 (TNM1) devoid of flagellar antigen. This vaccine did not elicit an H antibody response, but significant Vi and O agglutinin responses were observed. There were 34 typhoid cases among 21 063 six- to seven-year-old children who received the TNM1 vaccine, and 44 cases among 21 017 children in the control group who received tetanus toxoid. These results suggest that TNM1 vaccine does not provide protection against typhoid fever, and that H antigen may be an important component of an effective vaccine.", "contents": "Controlled field trial of a typhoid vaccine prepared with a nonmotile mutant of Salmonella typhi Ty2. A controlled field trial was performed in Egypt to evaluate a whole cell typhoid vaccine prepared with a nonmotile mutant of S. typhi Ty2 (TNM1) devoid of flagellar antigen. This vaccine did not elicit an H antibody response, but significant Vi and O agglutinin responses were observed. There were 34 typhoid cases among 21 063 six- to seven-year-old children who received the TNM1 vaccine, and 44 cases among 21 017 children in the control group who received tetanus toxoid. These results suggest that TNM1 vaccine does not provide protection against typhoid fever, and that H antigen may be an important component of an effective vaccine."}
{"id": "PMID:1082384", "title": "Control of Schistosoma mansoni transmission by provision of domestic water supplies. A preliminary report of a study in St Lucia.", "content": "As an experimental control measure to reduce the transmission of S. mansoni, an individual household water supply was provided in 400 houses in 5 rural settlements of the Riche Fond Valley, St Lucia. This population of about 2 000 had previously been dependent for water on infective streams and rivers. Six other settlements in the valley, all provided with limited piped water from public standpipes, served as the comparison area. After 2 years the incidence, prevalence, and intensity of infection with S. mansoni were significantly lower in the household water supply area, whereas all these indices of infection had increased in the comparison area. An adequate, reliable, and convenient supply of water can reduce the transmission of S. mansoni and should be considered as a control measure in other endemic areas.", "contents": "Control of Schistosoma mansoni transmission by provision of domestic water supplies. A preliminary report of a study in St Lucia. As an experimental control measure to reduce the transmission of S. mansoni, an individual household water supply was provided in 400 houses in 5 rural settlements of the Riche Fond Valley, St Lucia. This population of about 2 000 had previously been dependent for water on infective streams and rivers. Six other settlements in the valley, all provided with limited piped water from public standpipes, served as the comparison area. After 2 years the incidence, prevalence, and intensity of infection with S. mansoni were significantly lower in the household water supply area, whereas all these indices of infection had increased in the comparison area. An adequate, reliable, and convenient supply of water can reduce the transmission of S. mansoni and should be considered as a control measure in other endemic areas."}
{"id": "PMID:1082385", "title": "Inheritance of resistance to organophosphorus insecticides in Culex tarsalis Coquillet.", "content": "The mode of inheritance of resistance to parathion, fenitrothion, fenthion, chlorpyr fosmethyl, and parathion-methyl was studied in a strain of C. tarsalis. Resistance in the F(1) hybrid was partially dominant. Backcross experiments indicated that resistance is derived from more than one gene. This conclusion was confirmed by repeated backcrosses of the F(1) to the susceptible strain and selection of the backcross progeny with a discriminating dosage of parathion-methyl.", "contents": "Inheritance of resistance to organophosphorus insecticides in Culex tarsalis Coquillet. The mode of inheritance of resistance to parathion, fenitrothion, fenthion, chlorpyr fosmethyl, and parathion-methyl was studied in a strain of C. tarsalis. Resistance in the F(1) hybrid was partially dominant. Backcross experiments indicated that resistance is derived from more than one gene. This conclusion was confirmed by repeated backcrosses of the F(1) to the susceptible strain and selection of the backcross progeny with a discriminating dosage of parathion-methyl."}
{"id": "PMID:1082386", "title": "Left ventricular end-diastolic pressure volume relationships with experimental acute global ischemia.", "content": "The mechanism of elevation of left ventricular end-diastolic pressure during acute global ischemia was evaluated by examiniation of the relative contributions of a decrease in contractility and an alteration of the pressure-volume relationship. The external circumference (mercury-in-silastic gauge) pressure relationship, as an index of the pressure-volume relationship, was studied in beta adrenergic and ganglionic blocked, open chest dogs on right heart bypass at constant heart rate ane aortic pressure. Ischemia of one and two hours' duration was produced by reducing total coronary blood flow in cannulated left and right coronary arteries until left ventricular end-diastolic pressure rose significantly. At a constant stroke work, left ventricular end-diastolic pressure rose from 5.0 +/- 0.5 to 15.0 +/- 0.5 cm H2O in the experiments of one hour of ischemia, and from 7.0 +/- 1.0 to 17.0 +/- 1.0 cm H2O in experiments of two hours of ischemia. Ischemia was followed by one hour of restoration of coronary blood flow. Ischemia produced a marked depression of ventricular function: stroke work, considered at a left ventricular end-diastolic pressure of 15 cm H2O, decreased from 21.0 +/- 3.0 to 3.5 +/- 0.5 gm-m, and from 15.0 +/- 2.0 to 2.5 +/- 0.5 gm-m, in the experiments of one and two hours, respectively. Neither ischemia nor reflow changed the pressure-volume relationship. Thus, the elevation of left ventricular end-diastolic pressure during ischemia in an otherwise normal canine myocardium is due to a decrease in systolic performance of the heart rather than to an alteration of the pressure-volume relationship.", "contents": "Left ventricular end-diastolic pressure volume relationships with experimental acute global ischemia. The mechanism of elevation of left ventricular end-diastolic pressure during acute global ischemia was evaluated by examiniation of the relative contributions of a decrease in contractility and an alteration of the pressure-volume relationship. The external circumference (mercury-in-silastic gauge) pressure relationship, as an index of the pressure-volume relationship, was studied in beta adrenergic and ganglionic blocked, open chest dogs on right heart bypass at constant heart rate ane aortic pressure. Ischemia of one and two hours' duration was produced by reducing total coronary blood flow in cannulated left and right coronary arteries until left ventricular end-diastolic pressure rose significantly. At a constant stroke work, left ventricular end-diastolic pressure rose from 5.0 +/- 0.5 to 15.0 +/- 0.5 cm H2O in the experiments of one hour of ischemia, and from 7.0 +/- 1.0 to 17.0 +/- 1.0 cm H2O in experiments of two hours of ischemia. Ischemia was followed by one hour of restoration of coronary blood flow. Ischemia produced a marked depression of ventricular function: stroke work, considered at a left ventricular end-diastolic pressure of 15 cm H2O, decreased from 21.0 +/- 3.0 to 3.5 +/- 0.5 gm-m, and from 15.0 +/- 2.0 to 2.5 +/- 0.5 gm-m, in the experiments of one and two hours, respectively. Neither ischemia nor reflow changed the pressure-volume relationship. Thus, the elevation of left ventricular end-diastolic pressure during ischemia in an otherwise normal canine myocardium is due to a decrease in systolic performance of the heart rather than to an alteration of the pressure-volume relationship."}
{"id": "PMID:1082388", "title": "Enhanced uptake by guinea-pig macrophages of radio-iodinated human aggregated immunoglobulin G in the presence of sera from rheumatoid patients with cutaneous vasculitis.", "content": "A competitive radiobioassay method for soluble immune complexes in sera has been used to study sera from patients with rheumatoid arthritis. Twenty-eight out of sixty-two sera from selected seropositive rheumatoid patients were found to produce enhanced uptake of radio-iodinated human aggregated IgG by guinea-pig macrophages in contrast with the inhibition of uptake seen with SLE sera. Twenty-two out of the twenty-eight enhancing sera belonged to patients with cutaneous vasculitis. None of the twenty-two sera from seronegative rheumatoid patients possessed enhancing properties and none of these patients had clinical evidence of vasculitis. Three patients with seropositive SLE had sera with enhancing properties and had cutaneous vasculitis. We have shown that the enhancement is probably due to the presence in sera of immune complexes or altered IgG bound to rheumatoid factor. The possible mechanism has been investigated and it appears that there is increased binding of radio-iodinated aggregated IgG to free valencies on rheumatoid factor already bound to immune complexes.", "contents": "Enhanced uptake by guinea-pig macrophages of radio-iodinated human aggregated immunoglobulin G in the presence of sera from rheumatoid patients with cutaneous vasculitis. A competitive radiobioassay method for soluble immune complexes in sera has been used to study sera from patients with rheumatoid arthritis. Twenty-eight out of sixty-two sera from selected seropositive rheumatoid patients were found to produce enhanced uptake of radio-iodinated human aggregated IgG by guinea-pig macrophages in contrast with the inhibition of uptake seen with SLE sera. Twenty-two out of the twenty-eight enhancing sera belonged to patients with cutaneous vasculitis. None of the twenty-two sera from seronegative rheumatoid patients possessed enhancing properties and none of these patients had clinical evidence of vasculitis. Three patients with seropositive SLE had sera with enhancing properties and had cutaneous vasculitis. We have shown that the enhancement is probably due to the presence in sera of immune complexes or altered IgG bound to rheumatoid factor. The possible mechanism has been investigated and it appears that there is increased binding of radio-iodinated aggregated IgG to free valencies on rheumatoid factor already bound to immune complexes."}
{"id": "PMID:1082389", "title": "Thyrogastric autoimmune disease. Studies on the cell-mediated immune system and histocompatibility antigens.", "content": "Cell-mediated immune responses were studied in autoimmune diseases of thyrogastric type, Hashimoto's thyroiditis and autoimmune pernicious anaemia-type gastritis. Specific cell-mediated immunity was investigated by the leucocyte migration inhibition procedure, and general cell-mediated immunity (T-cell performance) was studied by standard in vivo and in vitro tests. In thyrogastric autoimmune diseases inhibition of migration of leucocytes was induced by thyroglobulin and gastric parietal cell microsomes; under conditions of presumably low cellular sensitization, stimulation of migration was observed. There was no depression of general cell-mediated immunity, in contrast to what occurs in systemic lupus erythematosus and related autoimmune diseases. A weak association of autoimmune gastritis with HL-A3 and HL-A7 (P LESS THAN 0.05) lost significance when an appropriate correction was applied; this weakness with HL-A clearly does not explain the strong genetic component in thyroid and gastric autoimmunity.", "contents": "Thyrogastric autoimmune disease. Studies on the cell-mediated immune system and histocompatibility antigens. Cell-mediated immune responses were studied in autoimmune diseases of thyrogastric type, Hashimoto's thyroiditis and autoimmune pernicious anaemia-type gastritis. Specific cell-mediated immunity was investigated by the leucocyte migration inhibition procedure, and general cell-mediated immunity (T-cell performance) was studied by standard in vivo and in vitro tests. In thyrogastric autoimmune diseases inhibition of migration of leucocytes was induced by thyroglobulin and gastric parietal cell microsomes; under conditions of presumably low cellular sensitization, stimulation of migration was observed. There was no depression of general cell-mediated immunity, in contrast to what occurs in systemic lupus erythematosus and related autoimmune diseases. A weak association of autoimmune gastritis with HL-A3 and HL-A7 (P LESS THAN 0.05) lost significance when an appropriate correction was applied; this weakness with HL-A clearly does not explain the strong genetic component in thyroid and gastric autoimmunity."}
{"id": "PMID:1082390", "title": "Correlations among cutaneous reactivity to DNCB, PHA-induced lymphocyte blastogenesis and peripheral blood E rosettes.", "content": "Comparisons of the results obtained in a study of fifty-two patients with genitourinary malignancies using three assays to monitor the thymus-dependent immune system (delayed cutaneous hypersensitivity to DNCB, PHA-induced lymphocyte blastogenesis, and peripheral blood E rosette-forming lymphocyte counts) yielded statistically significant positive correlations between the DNCB and PHA assays in twenty-one of twenty-eight instances, the DNCB and E rosette assays in thirteen of sixteen instances, the PHA and E rosette assays in twenty-eight of thirty-six instances, and among the DNCB, PHA and E rosette assays in ten of fourteen instances. The results suggest that both PHA-stimulated lymphocyte blastogenesis and peripheral blood E rosette-forming lymphocyte levels provide meaningful in vitro correlates of cell-mediated immunity.", "contents": "Correlations among cutaneous reactivity to DNCB, PHA-induced lymphocyte blastogenesis and peripheral blood E rosettes. Comparisons of the results obtained in a study of fifty-two patients with genitourinary malignancies using three assays to monitor the thymus-dependent immune system (delayed cutaneous hypersensitivity to DNCB, PHA-induced lymphocyte blastogenesis, and peripheral blood E rosette-forming lymphocyte counts) yielded statistically significant positive correlations between the DNCB and PHA assays in twenty-one of twenty-eight instances, the DNCB and E rosette assays in thirteen of sixteen instances, the PHA and E rosette assays in twenty-eight of thirty-six instances, and among the DNCB, PHA and E rosette assays in ten of fourteen instances. The results suggest that both PHA-stimulated lymphocyte blastogenesis and peripheral blood E rosette-forming lymphocyte levels provide meaningful in vitro correlates of cell-mediated immunity."}
{"id": "PMID:1082391", "title": "C3 receptor. A marker of a thymus-dependent B-cell subpopulation.", "content": "Depletion of C3-binding cells from spleen virtually abolishes the T cell-dependent response to sheep erythrocytes as assessed by transfer experiments. It is suggested that a functionally thymus-dependent B-cell subpopulation (B2) can be distinguished by surface receptors for C3.", "contents": "C3 receptor. A marker of a thymus-dependent B-cell subpopulation. Depletion of C3-binding cells from spleen virtually abolishes the T cell-dependent response to sheep erythrocytes as assessed by transfer experiments. It is suggested that a functionally thymus-dependent B-cell subpopulation (B2) can be distinguished by surface receptors for C3."}
{"id": "PMID:1082392", "title": "Effect of azathioprine on in vitro antibody response. Differential effect on B cells involved in thymus-dependent and independent responses.", "content": "The effect of azathioprine (Az) on the primary in vitro antibody response of mouse spleen cell cultures has been studied. The response towards T cell-dependent antigens is suppressed by low Az concentrations (50% inhibition by 10(-2) mug/ml and 100% suppression by 10(-1) mug/ml). The same pattern is observed when Az addition is delayed until day 2, but the suppression is absent or partial when Az is added on day 3. In the early period (day 0 to day 1) the effect of Az is reversible upon addition of an excess of purine nucleosides. In contrast, the response to a T cell-independent antigen (TNP-T4) is relatively insensitive to Az, since 100-fold higher drug concentrations are required to obtain an inhibition. With the assumption that T helper cells are likely to be highly sensitive to Az, the effect of low Az concentrations on the other two cell populations involved in T cell-dependent responses has been evaluated. Adherent cells appear unaffected. In contrast, the B-cell response is markedly sensitive to Az, as shown by the effect of Az on the response of nude mouse cells to a T cell-dependent antigen in the presence of T-cell products, either specific or non-specific. On the other hand, the B-cell response to mitogens is resistant to az. Thus, Az has a differential effect on B-cell response according to the thymus dependency of the antigen. This may suggest the existence of two pathways for B-cell activation or two different B-cell subpopulations.", "contents": "Effect of azathioprine on in vitro antibody response. Differential effect on B cells involved in thymus-dependent and independent responses. The effect of azathioprine (Az) on the primary in vitro antibody response of mouse spleen cell cultures has been studied. The response towards T cell-dependent antigens is suppressed by low Az concentrations (50% inhibition by 10(-2) mug/ml and 100% suppression by 10(-1) mug/ml). The same pattern is observed when Az addition is delayed until day 2, but the suppression is absent or partial when Az is added on day 3. In the early period (day 0 to day 1) the effect of Az is reversible upon addition of an excess of purine nucleosides. In contrast, the response to a T cell-independent antigen (TNP-T4) is relatively insensitive to Az, since 100-fold higher drug concentrations are required to obtain an inhibition. With the assumption that T helper cells are likely to be highly sensitive to Az, the effect of low Az concentrations on the other two cell populations involved in T cell-dependent responses has been evaluated. Adherent cells appear unaffected. In contrast, the B-cell response is markedly sensitive to Az, as shown by the effect of Az on the response of nude mouse cells to a T cell-dependent antigen in the presence of T-cell products, either specific or non-specific. On the other hand, the B-cell response to mitogens is resistant to az. Thus, Az has a differential effect on B-cell response according to the thymus dependency of the antigen. This may suggest the existence of two pathways for B-cell activation or two different B-cell subpopulations."}
{"id": "PMID:1082393", "title": "Blastogenic response of Toxoplasma-infected mouse spleen cells to T- and B-cell mitogens.", "content": "In order to differentially test the function of lymphocytes in Toxoplasma gondii-infected mice, the in vitro blastogenic response of spleen cell cultures to non-specific mitogens was studied. Phytohaemagglutinin (PHA) and concanavalin A (Con A) stimulation were used as tests of thymus-dependent lymphocyte (T cell) function and bacterial endotoxin lipopolysaccharide (LPS) was used as a probe of bursal equivalent lymphocyte (B cell) function. For the first 3 weeks following T. gondii infection, the uptake of tritiated thymidine ([3H]TdR) by spleen cells cultured with all three mitogens was markedly reduced in comparison to the uptake in spleen cells from uninfected control mice. Thereafter, the response to LPS returned to normal while stimulation by the T-cell mitogens (PHA and Con A) remained depressed. It is postulated that T. gondii infection either: (1) diluted out T cells in the spleen with unreactive cells; (2) modified T cells in such a way that they were less responsive to mitogens; (3) depleted the peripheral lymphoid tissues of T cells; (4) induced non-specific suppressor cells, which inhibited the T-cell function assays; or (5) activated macrophages which depressed T-cell function non-specifically.", "contents": "Blastogenic response of Toxoplasma-infected mouse spleen cells to T- and B-cell mitogens. In order to differentially test the function of lymphocytes in Toxoplasma gondii-infected mice, the in vitro blastogenic response of spleen cell cultures to non-specific mitogens was studied. Phytohaemagglutinin (PHA) and concanavalin A (Con A) stimulation were used as tests of thymus-dependent lymphocyte (T cell) function and bacterial endotoxin lipopolysaccharide (LPS) was used as a probe of bursal equivalent lymphocyte (B cell) function. For the first 3 weeks following T. gondii infection, the uptake of tritiated thymidine ([3H]TdR) by spleen cells cultured with all three mitogens was markedly reduced in comparison to the uptake in spleen cells from uninfected control mice. Thereafter, the response to LPS returned to normal while stimulation by the T-cell mitogens (PHA and Con A) remained depressed. It is postulated that T. gondii infection either: (1) diluted out T cells in the spleen with unreactive cells; (2) modified T cells in such a way that they were less responsive to mitogens; (3) depleted the peripheral lymphoid tissues of T cells; (4) induced non-specific suppressor cells, which inhibited the T-cell function assays; or (5) activated macrophages which depressed T-cell function non-specifically."}
{"id": "PMID:1082394", "title": "Increased sensitivity of the rosette-forming reaction of human T lymphocytes with sheep erythrocytes afforded by papain treatment of the sheep cells.", "content": "Human T lymphocytes are known to adhere to sheep erythrocytes. This affinity reaction was found to be consistently increased following treatment of the sheep cells with papain. Reactions with treated erythrocytes were more stable and involved higher percentages of lymphocytes. Using a double marker system of papain-treated sheep erythrocytes together with Fc rosettes as a presumptive B-cell marker, there was an overlap population of only 1% lymphocytes. Sheep erythrocytes treated with papain therefore appear to remain specifically adherent to human T lymphocytes.", "contents": "Increased sensitivity of the rosette-forming reaction of human T lymphocytes with sheep erythrocytes afforded by papain treatment of the sheep cells. Human T lymphocytes are known to adhere to sheep erythrocytes. This affinity reaction was found to be consistently increased following treatment of the sheep cells with papain. Reactions with treated erythrocytes were more stable and involved higher percentages of lymphocytes. Using a double marker system of papain-treated sheep erythrocytes together with Fc rosettes as a presumptive B-cell marker, there was an overlap population of only 1% lymphocytes. Sheep erythrocytes treated with papain therefore appear to remain specifically adherent to human T lymphocytes."}
{"id": "PMID:1082395", "title": "T and B lymphocytes in multiple sclerosis.", "content": "The percentage and total number of E and EAC rosettes, as indicators of T and B lymphocytes respectively, were studied in the blood of subjects with multiple sclerosis (MS) and normals. MS patients in acute exacerbation were found to have a decrease in E rosettes and an increase in EAC rosettes. The relationship of these findings to the pathogenesis of MS is unclear; several possible pathogenetic implications are considered.", "contents": "T and B lymphocytes in multiple sclerosis. The percentage and total number of E and EAC rosettes, as indicators of T and B lymphocytes respectively, were studied in the blood of subjects with multiple sclerosis (MS) and normals. MS patients in acute exacerbation were found to have a decrease in E rosettes and an increase in EAC rosettes. The relationship of these findings to the pathogenesis of MS is unclear; several possible pathogenetic implications are considered."}
{"id": "PMID:1082396", "title": "Cell-mediated immunity in patients on long-term haemodialysis.", "content": "Several parameters of cell-mediated immunity were assessed in thirty patients with chronic renal failure treated with long-term haemodialysis. Lymphopenia was uncommon, and only two patients showed diminished numbers of thymus-derived peripheral blood lymphocytes. Skin test energy to three antigens was documented in only one patient. No serious infections with intracellular organisms were noted in any of the patients. Phytohaemagglutinin (PHA) induced DNA synthesis in eleven patients was lower than that shown by normal subjects. This poor responsiveness to PHA was not corrected by haemodialysis, and in only two patients was the response improved by substituting normal human plasma for uraemic plasma in the lymphocyte cultures. Thus with the exception of PHA response, other parameters of cell-mediated immunity were intact in this group of chronic stable haemodialysis patients.", "contents": "Cell-mediated immunity in patients on long-term haemodialysis. Several parameters of cell-mediated immunity were assessed in thirty patients with chronic renal failure treated with long-term haemodialysis. Lymphopenia was uncommon, and only two patients showed diminished numbers of thymus-derived peripheral blood lymphocytes. Skin test energy to three antigens was documented in only one patient. No serious infections with intracellular organisms were noted in any of the patients. Phytohaemagglutinin (PHA) induced DNA synthesis in eleven patients was lower than that shown by normal subjects. This poor responsiveness to PHA was not corrected by haemodialysis, and in only two patients was the response improved by substituting normal human plasma for uraemic plasma in the lymphocyte cultures. Thus with the exception of PHA response, other parameters of cell-mediated immunity were intact in this group of chronic stable haemodialysis patients."}
{"id": "PMID:1082397", "title": "The value of tests for antibodies to DNA in monitoring the clinical course of SLE. A long-term study using the Farr test and the DNA counterimmunoelectrophoretic method.", "content": "Serial serum samples from fifteen patients with SLE, taken over periods varying from 6 months to 6 years, were tested for DNA binding capacity, DNA electroprecipitins (DNA-EP) and C'3 level to assess the value of these investigations in reflecting clinical disease activity. Patients with renal involvement showed a good correlation between high levels of DNA binding, low serum C'3 and disease activity and typically, their DNA-EP was negative. By contrast, patients without renal involvement in whom vasculitis was prominent, showed a poor correlation of DNA binding capacity to changes in the state of their disease although the DNA-EP test was persistently positive. It was also apparent that both the DNA-BC and C'3 can show marked variation in response to alterations in treatment without accompanying clinical change. Although these serological tests, particularly the DNA binding capacity are of recognized value in the diagnosis of SLE, they serve most usefully as guides to long-term management when they can be related to the clinical pattern of the disease.", "contents": "The value of tests for antibodies to DNA in monitoring the clinical course of SLE. A long-term study using the Farr test and the DNA counterimmunoelectrophoretic method. Serial serum samples from fifteen patients with SLE, taken over periods varying from 6 months to 6 years, were tested for DNA binding capacity, DNA electroprecipitins (DNA-EP) and C'3 level to assess the value of these investigations in reflecting clinical disease activity. Patients with renal involvement showed a good correlation between high levels of DNA binding, low serum C'3 and disease activity and typically, their DNA-EP was negative. By contrast, patients without renal involvement in whom vasculitis was prominent, showed a poor correlation of DNA binding capacity to changes in the state of their disease although the DNA-EP test was persistently positive. It was also apparent that both the DNA-BC and C'3 can show marked variation in response to alterations in treatment without accompanying clinical change. Although these serological tests, particularly the DNA binding capacity are of recognized value in the diagnosis of SLE, they serve most usefully as guides to long-term management when they can be related to the clinical pattern of the disease."}
{"id": "PMID:1082398", "title": "Comparative mapping of the local distribution of immunoglobulin-containing cells in ulcerative colitis and Crohn's disease of the colon.", "content": "The local response pattern of immunoglobulin-containing cells was compared in Crohn's disease and ulcerative colitis by paired immunohistochemistry on specimens of the large bowel wall. In the \"Crohn mucosa\" with persisting glands the total cell count was on the average raised more than three times compared with controls. The numbers of IgA, IgM and IgG immunocytes were increased 2.0, 4.8 and 28.6 times, respectively. Only 0-2 IgD- and IgE-containing cells were generally found per section. No consistent differences in the mucosal response pattern were revealed when Crohn's disease was compared with ulcerative colitis. The deeper layers of the bowel wall were in both diseases more or less densely infiltrated by immunocytes-IgG cells compromising about 80%. Immunoglobulin-containing cells in the muscularis propria and subserosa were characteristically found in Crohn's disease. There was no indication of a primary defect in the secretory immunoglobulin system which appeared to be normal in areas with intact glands. The pronounced local humoral immune response, particularly that involving IgG, might be of pathogenetic importance by aggravating and perpetuating in the inflammatory bowel disease.", "contents": "Comparative mapping of the local distribution of immunoglobulin-containing cells in ulcerative colitis and Crohn's disease of the colon. The local response pattern of immunoglobulin-containing cells was compared in Crohn's disease and ulcerative colitis by paired immunohistochemistry on specimens of the large bowel wall. In the \"Crohn mucosa\" with persisting glands the total cell count was on the average raised more than three times compared with controls. The numbers of IgA, IgM and IgG immunocytes were increased 2.0, 4.8 and 28.6 times, respectively. Only 0-2 IgD- and IgE-containing cells were generally found per section. No consistent differences in the mucosal response pattern were revealed when Crohn's disease was compared with ulcerative colitis. The deeper layers of the bowel wall were in both diseases more or less densely infiltrated by immunocytes-IgG cells compromising about 80%. Immunoglobulin-containing cells in the muscularis propria and subserosa were characteristically found in Crohn's disease. There was no indication of a primary defect in the secretory immunoglobulin system which appeared to be normal in areas with intact glands. The pronounced local humoral immune response, particularly that involving IgG, might be of pathogenetic importance by aggravating and perpetuating in the inflammatory bowel disease."}
{"id": "PMID:1082399", "title": "Activation of human peripheral blood lymphocytes by concanavalin A dependence of monocytes.", "content": "The activation of human peripheral blood lymphocytes or isolated T lymphocytes by concanavalin A (Con A) is hightly potentiated by the presence of autologous, mitomycin C-treated monocytes. The optimal lymphocyte: monocyte ratio within a broad dose range is 1:1 when the incorporation of [14C]thymidine is expressed as total incorporation per culture tube and 1:10 when expressed per lymphocyte. A five-to-ten-fold increase of total DNA synthesis is noted in the presence of 10-90% monocytes. The data may help to explain the wide variations in Con A responsiveness of human peripheral lymphocytes which may be partly related to differences in purification which give rise to cell preparations containing varying amounts of monocytes.", "contents": "Activation of human peripheral blood lymphocytes by concanavalin A dependence of monocytes. The activation of human peripheral blood lymphocytes or isolated T lymphocytes by concanavalin A (Con A) is hightly potentiated by the presence of autologous, mitomycin C-treated monocytes. The optimal lymphocyte: monocyte ratio within a broad dose range is 1:1 when the incorporation of [14C]thymidine is expressed as total incorporation per culture tube and 1:10 when expressed per lymphocyte. A five-to-ten-fold increase of total DNA synthesis is noted in the presence of 10-90% monocytes. The data may help to explain the wide variations in Con A responsiveness of human peripheral lymphocytes which may be partly related to differences in purification which give rise to cell preparations containing varying amounts of monocytes."}
{"id": "PMID:1082400", "title": "Effect of radiotherapy on lymphocyte cytotoxicity in vitro.", "content": "The cytotoxic functions of highly purified blood lymphocytes from patients with breast cancer were studied before and after radiotherapy. Addition of PHA or of rabbit antibodies to target cells (chicken erythrocytes) were chosen as two means of inducing lymphocyte cytotoxicity in vitro. The proportion of T and non-T-lymphocytes was determined by means of E and EAC rosette tests. The antibody-induced cytotoxocity of lymphocytes decreased following radiotherapy while that mediated by PHA remained unchanged. There was some reduction in the percentage of EAC rosette-forming cells. These results, as well as our earlier observations, suggest that the decrease in the peripheral blood of the proportion of lymphocytes with receptors for activated complement is responsible for changes in the antibody-mediated lymphocyte cytotoxicity.", "contents": "Effect of radiotherapy on lymphocyte cytotoxicity in vitro. The cytotoxic functions of highly purified blood lymphocytes from patients with breast cancer were studied before and after radiotherapy. Addition of PHA or of rabbit antibodies to target cells (chicken erythrocytes) were chosen as two means of inducing lymphocyte cytotoxicity in vitro. The proportion of T and non-T-lymphocytes was determined by means of E and EAC rosette tests. The antibody-induced cytotoxocity of lymphocytes decreased following radiotherapy while that mediated by PHA remained unchanged. There was some reduction in the percentage of EAC rosette-forming cells. These results, as well as our earlier observations, suggest that the decrease in the peripheral blood of the proportion of lymphocytes with receptors for activated complement is responsible for changes in the antibody-mediated lymphocyte cytotoxicity."}
{"id": "PMID:1082401", "title": "In vitro lymphocyte reactivity and T-cell levels in chronic cigarette smokers.", "content": "Peripheral blood total leucocyte, lymphocyte and thymus-dependent lymphocyte (T cell) levels and in vitro lymphocyte reactivity (LR) to phytohaemagglutinin (PHA) were determined in 153 chronic cigarette smokers and 115 non-smokers ranging in age from 20 to 78 years. Total leucocyte, lymphocyte and T-cell levels were significantly elevated in smokers. There was no correlation with age. LR to PHA was significantly higher in smokers less than 40 years of age or in those with a 20 pack-year or less history of cigarette consumption. Older smokers or those with a history of heavier cigarette consumption did not differ from normals. The results contrast with previous demonstrations of suppression of immune reactivity after exposure to tobacco products. The possible effects of the apparent stimulation of the lymphoid system by chromic cigarette consumption is considered.", "contents": "In vitro lymphocyte reactivity and T-cell levels in chronic cigarette smokers. Peripheral blood total leucocyte, lymphocyte and thymus-dependent lymphocyte (T cell) levels and in vitro lymphocyte reactivity (LR) to phytohaemagglutinin (PHA) were determined in 153 chronic cigarette smokers and 115 non-smokers ranging in age from 20 to 78 years. Total leucocyte, lymphocyte and T-cell levels were significantly elevated in smokers. There was no correlation with age. LR to PHA was significantly higher in smokers less than 40 years of age or in those with a 20 pack-year or less history of cigarette consumption. Older smokers or those with a history of heavier cigarette consumption did not differ from normals. The results contrast with previous demonstrations of suppression of immune reactivity after exposure to tobacco products. The possible effects of the apparent stimulation of the lymphoid system by chromic cigarette consumption is considered."}
{"id": "PMID:1082402", "title": "Spontaneous autorosette-forming cells in man. A marker for a subset population of T lymphocytes?", "content": "A subpopulation of human peripheral blood lymphocytes are capable of binding with human (autologous or allogeneic) erythrocytes, forming rosettes. The conditions which lead to autorosette formation are similar to those required for sheep red-cell rosetting. Ageing human erythrocytes are shown to bear less of the determinants involved in the phenomenon than younger ones. Evidence is presented that autorosetting is a T-cell marker. As autorosette-forming cells are very sensitive to the inhibiting effects of ATG they could therefore belong to a T-cell subpopulation.", "contents": "Spontaneous autorosette-forming cells in man. A marker for a subset population of T lymphocytes? A subpopulation of human peripheral blood lymphocytes are capable of binding with human (autologous or allogeneic) erythrocytes, forming rosettes. The conditions which lead to autorosette formation are similar to those required for sheep red-cell rosetting. Ageing human erythrocytes are shown to bear less of the determinants involved in the phenomenon than younger ones. Evidence is presented that autorosetting is a T-cell marker. As autorosette-forming cells are very sensitive to the inhibiting effects of ATG they could therefore belong to a T-cell subpopulation."}
{"id": "PMID:1082403", "title": "Hyperimmunoglobulinaemia, T-cell deficiency and plasmacytosis in RFM mice with host versus graft disease induced by the perinatal inoculations (T6XRFM)F1 spleen cells.", "content": "Host versus graft (HVG) syndrome may be induced in parental strain mice by perinatal inoculations of F1 hybrid spleen cells. The principal manifestations of the disease include thrombocytopaenia, intravascular fibrin deposits, intestinal haemorrhage, hepatic infarcts, lymphosplenomegaly and renal disease. Immune complexes have been shown to be the cause of the renal lesions, and have been implicated as the triggers for disseminated intravascular coagulation. In the present studies of RFM mice perinatally inoculated with (T6 x RFM)F1 spleen cells (RFM/(T6 x RFM)F1 mice), quantitative determinations of serum immunoglobulins (Ig) revealed marked elevations of IgG1, IgG2, IgA and IgM. Electrophoretic analyses revealed the polyclonal pattern which typically follows chronic antigenic stimulation. However, IgG1 levels which reached 29 to 72 times control values suggested disruption of homeostatic mechanisms which control circulating Ig levels. Because antibody responses to histocompatibility antigens were present only occasionally, and then in low titre, it seemed unlikely these antigens were the principal causes of hypergammaglobulinaemia and plasmacytosis. Morphological studies indicated that the elevated levels of Ig seen in end-stage HVG syndrome correlated well with marked plasmacytosis, the third morphological finding in a sequence that included the precocious development of germinal centres and subsequent depletion of thymic-dependent (T) lymphocytes. The fact that spleen cells from RFM/(T6 x RFM)F1 mice were severely impaired in their capacity to cause graft versus host disease in related (T6 x RFM)F1 and unrelated C3H mice provided strong evidence that the HVG reaction resulted in T-cell depletion, rather than specific immunoincompetence.", "contents": "Hyperimmunoglobulinaemia, T-cell deficiency and plasmacytosis in RFM mice with host versus graft disease induced by the perinatal inoculations (T6XRFM)F1 spleen cells. Host versus graft (HVG) syndrome may be induced in parental strain mice by perinatal inoculations of F1 hybrid spleen cells. The principal manifestations of the disease include thrombocytopaenia, intravascular fibrin deposits, intestinal haemorrhage, hepatic infarcts, lymphosplenomegaly and renal disease. Immune complexes have been shown to be the cause of the renal lesions, and have been implicated as the triggers for disseminated intravascular coagulation. In the present studies of RFM mice perinatally inoculated with (T6 x RFM)F1 spleen cells (RFM/(T6 x RFM)F1 mice), quantitative determinations of serum immunoglobulins (Ig) revealed marked elevations of IgG1, IgG2, IgA and IgM. Electrophoretic analyses revealed the polyclonal pattern which typically follows chronic antigenic stimulation. However, IgG1 levels which reached 29 to 72 times control values suggested disruption of homeostatic mechanisms which control circulating Ig levels. Because antibody responses to histocompatibility antigens were present only occasionally, and then in low titre, it seemed unlikely these antigens were the principal causes of hypergammaglobulinaemia and plasmacytosis. Morphological studies indicated that the elevated levels of Ig seen in end-stage HVG syndrome correlated well with marked plasmacytosis, the third morphological finding in a sequence that included the precocious development of germinal centres and subsequent depletion of thymic-dependent (T) lymphocytes. The fact that spleen cells from RFM/(T6 x RFM)F1 mice were severely impaired in their capacity to cause graft versus host disease in related (T6 x RFM)F1 and unrelated C3H mice provided strong evidence that the HVG reaction resulted in T-cell depletion, rather than specific immunoincompetence."}
{"id": "PMID:1082404", "title": "Thymus dependence of the antibody response to tetanus toxoid in mice.", "content": "Both the IgM and IgG antibody responses to tetanus toxoid were greatly reduced in adult thymectomized, irradiated, foetal liver reconstituted mice, in contrast with results published for neonatally thymectomized mice. This evidence lends further support to the notion that all IgG responses to protein antigens are thymus dependent.", "contents": "Thymus dependence of the antibody response to tetanus toxoid in mice. Both the IgM and IgG antibody responses to tetanus toxoid were greatly reduced in adult thymectomized, irradiated, foetal liver reconstituted mice, in contrast with results published for neonatally thymectomized mice. This evidence lends further support to the notion that all IgG responses to protein antigens are thymus dependent."}
{"id": "PMID:1082410", "title": "Complete obstruction of the left main coronary artery associated with congenital pulmonary valvular stenosis.", "content": "The surgical treatment of the completely obstructed left main coronary artery is aortocoronary saphenous-vein bypass to the left coronary artery system. Without surgical intervention, this lesion has an exceedingly poor prognosis. This report describes a patient with atherosclerotic obstruction of the left main coronary artery and congenital pulmonary valvular stenosis, an unusual combination of lesions not previously documented together. The patient remains asymptomatic 22 months after aortocoronary saphenous-vein bypass and pulmonary valvotomy.", "contents": "Complete obstruction of the left main coronary artery associated with congenital pulmonary valvular stenosis. The surgical treatment of the completely obstructed left main coronary artery is aortocoronary saphenous-vein bypass to the left coronary artery system. Without surgical intervention, this lesion has an exceedingly poor prognosis. This report describes a patient with atherosclerotic obstruction of the left main coronary artery and congenital pulmonary valvular stenosis, an unusual combination of lesions not previously documented together. The patient remains asymptomatic 22 months after aortocoronary saphenous-vein bypass and pulmonary valvotomy."}
{"id": "PMID:1082409", "title": "A system-structured medical record for intensive care patient documentation.", "content": "The problem-oriented approach to the medical record has aroused a long overdue interest in the structuring of the medical case file. Clinical information in the traditional record is source-structured and time-sequenced, whereas the problem-oriented system differs by being a problem-structured record retaining still a chronologic sequence. We have found that in acute illness the multiplicity of interacting pathophysiologic processes makes premature application of the problem-oriented approach cumbersome and unwieldy. The formulation of the problem list at an early stage often led to the reduplication of problems, creating disorder in the clinical picture rather than serving to clarify it. Some used the simple cataloguing of events and data as a substitute for clinical judgment and decision making, focusing more upon style rather than content of the medical record. By using a rigid physiologic system-structured \"problem\" list and a modification of the SOAP (Subjective Objective, Assessment, Plan) subdivision, we have improved the documentation of our intensive care patients. The summary of the patient's stay in the intensive care unit is structured with active and inactive problems, this summary to be further used as the permanent problem list.", "contents": "A system-structured medical record for intensive care patient documentation. The problem-oriented approach to the medical record has aroused a long overdue interest in the structuring of the medical case file. Clinical information in the traditional record is source-structured and time-sequenced, whereas the problem-oriented system differs by being a problem-structured record retaining still a chronologic sequence. We have found that in acute illness the multiplicity of interacting pathophysiologic processes makes premature application of the problem-oriented approach cumbersome and unwieldy. The formulation of the problem list at an early stage often led to the reduplication of problems, creating disorder in the clinical picture rather than serving to clarify it. Some used the simple cataloguing of events and data as a substitute for clinical judgment and decision making, focusing more upon style rather than content of the medical record. By using a rigid physiologic system-structured \"problem\" list and a modification of the SOAP (Subjective Objective, Assessment, Plan) subdivision, we have improved the documentation of our intensive care patients. The summary of the patient's stay in the intensive care unit is structured with active and inactive problems, this summary to be further used as the permanent problem list."}
{"id": "PMID:1082415", "title": "[The quantitative determination of the individual CSF proteins in the diagnosis of inflammatory diseases of the CNS in children (author's transl)].", "content": "Albumin, IgG, IgA, IgM, transferrin, and alpha 1-antitrypsin were determined quantitatively in the cerebrospinal fluid (CSF) of 44 healthy children and 37 pediatric patients with central nervous system diseases. Neither IgA nor IgM were found in the CSF of normal children, but they were present in cases of purulent and non-bacterial meningitis. In cases of encephalitis all proteins studied were increased except IgA and IgM, which could not be demonstrated. On the basis of the results it may be concluded that the increase of IgG in the CSF in bacterial and a bacterial meningitis is due to an increased permeability of the blood-CSF barrier. Except in cases with ependymitis, IgG is produced only in small amounts in or near the CSF spaces even in the presence of inflammation of the meninges. The quantitative determination of IgG, IgA, and IgM in case of increased CSF protein content may facilitate the differential diagnosis between purulent and acute viral meningitis and encephalitis.", "contents": "[The quantitative determination of the individual CSF proteins in the diagnosis of inflammatory diseases of the CNS in children (author's transl)]. Albumin, IgG, IgA, IgM, transferrin, and alpha 1-antitrypsin were determined quantitatively in the cerebrospinal fluid (CSF) of 44 healthy children and 37 pediatric patients with central nervous system diseases. Neither IgA nor IgM were found in the CSF of normal children, but they were present in cases of purulent and non-bacterial meningitis. In cases of encephalitis all proteins studied were increased except IgA and IgM, which could not be demonstrated. On the basis of the results it may be concluded that the increase of IgG in the CSF in bacterial and a bacterial meningitis is due to an increased permeability of the blood-CSF barrier. Except in cases with ependymitis, IgG is produced only in small amounts in or near the CSF spaces even in the presence of inflammation of the meninges. The quantitative determination of IgG, IgA, and IgM in case of increased CSF protein content may facilitate the differential diagnosis between purulent and acute viral meningitis and encephalitis."}
{"id": "PMID:1082419", "title": "Adrenaline and the electrogenic sodium pump in Rana catesbeiana sympathetic ganglion cells.", "content": "The effect of adrenaline on the Na+-pump in bullfrog (Rana catesbeiana) sympathetic ganglion cells was studied by use of electrophysiological methods. The rate of removal of excess Na+ injected into a ganglion cell was increased by adrenaline. The K+-activated hyperpolarization of cell membrane, which might be produced by an electrogenic Na+-pump, was also increased by adrenaline. These results suggested that adrenaline was able to accelerate the Na+-pump, possibly the electrogenic Na+-pump.", "contents": "Adrenaline and the electrogenic sodium pump in Rana catesbeiana sympathetic ganglion cells. The effect of adrenaline on the Na+-pump in bullfrog (Rana catesbeiana) sympathetic ganglion cells was studied by use of electrophysiological methods. The rate of removal of excess Na+ injected into a ganglion cell was increased by adrenaline. The K+-activated hyperpolarization of cell membrane, which might be produced by an electrogenic Na+-pump, was also increased by adrenaline. These results suggested that adrenaline was able to accelerate the Na+-pump, possibly the electrogenic Na+-pump."}
{"id": "PMID:1082421", "title": "[The role of the hypothalamus in the mechanism of positional nystagmus].", "content": "The typical positional nystagmus was induced in 30 intact rabbits with injection of 0.1-0.4 ml of 0.03-0.05% dicaine solution into the cerebral cisterna magna. The positional nystagmus resulted, supposedly, from the effect of the anesthetic upon the basal structures of the 3rd ventricle of the brain, upon the hypothalamus in particular. The trigger mechanism of the phenomenon is the displacement of the membraneous ampullae of semicircular canals at the tilting of the head. To prove this, 0.01-0.03 ml of 4% dicaine solution were injected directly into the anterior and posterior hypothalamus through a metallic cannula. When introduced into the anterior hypothalamus the dose of the anesthetic necessary for occurrence of the positional nystagmus is two or three times lesser than when it is injected into the posterior nypothalamus. In two delabyrinthed rabbits we failed to induce the nystagmus in this way.", "contents": "[The role of the hypothalamus in the mechanism of positional nystagmus]. The typical positional nystagmus was induced in 30 intact rabbits with injection of 0.1-0.4 ml of 0.03-0.05% dicaine solution into the cerebral cisterna magna. The positional nystagmus resulted, supposedly, from the effect of the anesthetic upon the basal structures of the 3rd ventricle of the brain, upon the hypothalamus in particular. The trigger mechanism of the phenomenon is the displacement of the membraneous ampullae of semicircular canals at the tilting of the head. To prove this, 0.01-0.03 ml of 4% dicaine solution were injected directly into the anterior and posterior hypothalamus through a metallic cannula. When introduced into the anterior hypothalamus the dose of the anesthetic necessary for occurrence of the positional nystagmus is two or three times lesser than when it is injected into the posterior nypothalamus. In two delabyrinthed rabbits we failed to induce the nystagmus in this way."}
{"id": "PMID:1082422", "title": "Differential effects of vasopressin on the water, calcium and lysosomal enzyme contents of mitochondria-rich and lysosome-rich (granular) epithelial cells isolated from bullfrog urinary bladder.", "content": "Treatment of bullfrog urinary bladder with arginine vasopressin (AVP) elicited a dose-dependent increase in the basal movement of water and sodium across isolated tissues. Epithelial cells from the mucosal surface and incubated with 10 mU AVP/ml for 30 min retained a greater amount of intracellular water and calcium than cells not treated with hormone. The epithelial cells were further separated into two major fractions by density gradient centrifugation; cells damaged during these manipulations were separated from viable cells and discarded. Morphologcal examination of the two respective fractions indicated that they largely consisted of mitochondria-rich (MR) and granular (G) cell types which line the lumen of bullfrog bladder. The calcium content of MR cells averaged 25% greater than that of G-type cells. G cells had a markedly higher content of the characteristic lysosomal hydrolases, acid phsophatase and cathepsin B1, than that found in MR cells. Incubation of G cells with AVP elicited significant increments in water and calcium contents and extracellular release of lysosomal enzymes as compared to untreated cells. Among MR cells treated with AVP, cell calcium declines slightly but no significant increase in water content or extracellular hydrolase activity was detected in comparison  with paired control cells. The physiological significance of acid proteinase release from G cells treated with AVP was evaluated in experiments with intact bladder. Proteinase inhibitors which suppress the activity of cathepsin B1 selectively antagonized the action of hormone on water permeation. The data suggest that alterations in the calcium and lysosomal hydrolase activity associated with G cells exposed to AVP may contribute to the hormone-induced water flow observed across the intact epithelium.", "contents": "Differential effects of vasopressin on the water, calcium and lysosomal enzyme contents of mitochondria-rich and lysosome-rich (granular) epithelial cells isolated from bullfrog urinary bladder. Treatment of bullfrog urinary bladder with arginine vasopressin (AVP) elicited a dose-dependent increase in the basal movement of water and sodium across isolated tissues. Epithelial cells from the mucosal surface and incubated with 10 mU AVP/ml for 30 min retained a greater amount of intracellular water and calcium than cells not treated with hormone. The epithelial cells were further separated into two major fractions by density gradient centrifugation; cells damaged during these manipulations were separated from viable cells and discarded. Morphologcal examination of the two respective fractions indicated that they largely consisted of mitochondria-rich (MR) and granular (G) cell types which line the lumen of bullfrog bladder. The calcium content of MR cells averaged 25% greater than that of G-type cells. G cells had a markedly higher content of the characteristic lysosomal hydrolases, acid phsophatase and cathepsin B1, than that found in MR cells. Incubation of G cells with AVP elicited significant increments in water and calcium contents and extracellular release of lysosomal enzymes as compared to untreated cells. Among MR cells treated with AVP, cell calcium declines slightly but no significant increase in water content or extracellular hydrolase activity was detected in comparison  with paired control cells. The physiological significance of acid proteinase release from G cells treated with AVP was evaluated in experiments with intact bladder. Proteinase inhibitors which suppress the activity of cathepsin B1 selectively antagonized the action of hormone on water permeation. The data suggest that alterations in the calcium and lysosomal hydrolase activity associated with G cells exposed to AVP may contribute to the hormone-induced water flow observed across the intact epithelium."}
{"id": "PMID:1082420", "title": "[Influence of imipramine on arousal from winter hibernation].", "content": "Imipramine (20 and 50 mg/kg intraperitoneally) is shown to delay the arousal of gophers from hibernation. This effect is similar to the action of serotonin (5 mg/kg) and 5-oxytryptophan (50 mg/kg). In the latter, however, the action on the thermogenesis is most pronounced and, because of this the warming up of the gophers is inhibited, while imipramine acts on the arousal. Tranylcypromine (20 mg/kg) failed to exert any substantial effect on the worming up and arousal of gophers from hibernation and attenuated the action of imipramine.", "contents": "[Influence of imipramine on arousal from winter hibernation]. Imipramine (20 and 50 mg/kg intraperitoneally) is shown to delay the arousal of gophers from hibernation. This effect is similar to the action of serotonin (5 mg/kg) and 5-oxytryptophan (50 mg/kg). In the latter, however, the action on the thermogenesis is most pronounced and, because of this the warming up of the gophers is inhibited, while imipramine acts on the arousal. Tranylcypromine (20 mg/kg) failed to exert any substantial effect on the worming up and arousal of gophers from hibernation and attenuated the action of imipramine."}
{"id": "PMID:1082423", "title": "In vitro studies of inflammatory bowel disease. Surface receptors of the mononuclear cell required to lyse allogeneic colonic epithelial cells.", "content": "Peripheral blood mononuclear cells from patients with either Crohn's disease or ulcerative colitis (collectively referred to as inflammatory bowel disease) are cytotoxic in vitro for isologous or allogeneic colonic epithelial cells. Utilizing the ability of thymus-derived (T) lymphocytes to bind sheep red blood cells to their surface and the property of bone marrow-derived (B) lymphocytes to display easily detectable surface immunoglobulin determinants, the cytoxicity of these lymphocyte subpopulations was tested. The results indicate that the mononuclear cell required for the lysis of colonic epithelium was not included within the bulk of T or B lymphocytes. Indeed, enrichment for cytotoxicity correlated best with enrichment for a mononuclear cell population lacking classical T or B markers. Additionally, mononuclear cells specifically adhering to plastic petri dishes coated with heat-aggregated immunoglobulin, and thus presumably bearing a surface Fc receptor, were enriched in their cytotoxicity. Alternatively, cells not adhering to aggregated Ig-coated petri dishes were relatively depleted of cytotoxicity. The implications of these findings as they relate to possible interactions between cellular and humoral immune mechanisms as a pathogenic mechanism for the colonic inflammatory process noted in patients with inflammatory bowel disease are discussed.", "contents": "In vitro studies of inflammatory bowel disease. Surface receptors of the mononuclear cell required to lyse allogeneic colonic epithelial cells. Peripheral blood mononuclear cells from patients with either Crohn's disease or ulcerative colitis (collectively referred to as inflammatory bowel disease) are cytotoxic in vitro for isologous or allogeneic colonic epithelial cells. Utilizing the ability of thymus-derived (T) lymphocytes to bind sheep red blood cells to their surface and the property of bone marrow-derived (B) lymphocytes to display easily detectable surface immunoglobulin determinants, the cytoxicity of these lymphocyte subpopulations was tested. The results indicate that the mononuclear cell required for the lysis of colonic epithelium was not included within the bulk of T or B lymphocytes. Indeed, enrichment for cytotoxicity correlated best with enrichment for a mononuclear cell population lacking classical T or B markers. Additionally, mononuclear cells specifically adhering to plastic petri dishes coated with heat-aggregated immunoglobulin, and thus presumably bearing a surface Fc receptor, were enriched in their cytotoxicity. Alternatively, cells not adhering to aggregated Ig-coated petri dishes were relatively depleted of cytotoxicity. The implications of these findings as they relate to possible interactions between cellular and humoral immune mechanisms as a pathogenic mechanism for the colonic inflammatory process noted in patients with inflammatory bowel disease are discussed."}
{"id": "PMID:1082424", "title": "Isolation and kinetic characteristics of mucosal lymphocytes in Crohn's Disease.", "content": "Single cell suspensions of lymphocytes have been prepared from human gut mucosa, and short-term culture used to characterize the kinetics of DNA synthesis of mucosal lymphocytes. High rates of DNA synthesis were found in 72-hr cultures of Crohn's disease mucosal lymphocytes, compared with normal subjects. These high rates correlated with the appearance of numerous transformed blast cells in terminated cultures. Lymphocytes from normal and abnormal human gut mucosa were stimulated by phytohemagglutinin-P, except in some patients with Crohn's disease with high rates of \"spontaneous\" DNA synthesis. The isolation and culture of human gut mucosal lymphocytes by a simple preparative technique will enable the direct study of lymphocyte function in both normal and diseased gut mucosa.", "contents": "Isolation and kinetic characteristics of mucosal lymphocytes in Crohn's Disease. Single cell suspensions of lymphocytes have been prepared from human gut mucosa, and short-term culture used to characterize the kinetics of DNA synthesis of mucosal lymphocytes. High rates of DNA synthesis were found in 72-hr cultures of Crohn's disease mucosal lymphocytes, compared with normal subjects. These high rates correlated with the appearance of numerous transformed blast cells in terminated cultures. Lymphocytes from normal and abnormal human gut mucosa were stimulated by phytohemagglutinin-P, except in some patients with Crohn's disease with high rates of \"spontaneous\" DNA synthesis. The isolation and culture of human gut mucosal lymphocytes by a simple preparative technique will enable the direct study of lymphocyte function in both normal and diseased gut mucosa."}
{"id": "PMID:1082425", "title": "Antibody-dependent cell-mediated cytotoxicity. Comparative ability of murine Peyer's patch and spleen cells to lyse lipopolysaccharide-coated and uncoated erythrocytes.", "content": "Lymphoid cells from nonsensitized donors can be cytotoxic in vitro for target cells coated with antibody. The antibody can be directed either against antigens which are intrinsic to or experimentally attached to the target cell surface. Killing of target cells by this method has been termed antibody-dependent cell-mediated cytotoxicity (ADCC). In these investigations we compared the ability of Peyer's patch cells and spleen cells from nonsensitized mice to lyse lipoplysaccharide-coated and uncoated target cells by an ADCC mechanism. The results demonstrate that spleen cells, but not Peyer's patch cells, from identical mice can be cytotoxic for lipopolysaccharide-coated chicken erythrocytes (CRBC) in the presence of anti-lipopolysaccharide antibody and uncoated CRBC in the presence of anti-CRBC antibody. Thus, Peyer's patches in mice appear to be deficient in the effector cell type(s) required to kill antibody-coated erythrocyte target cells by an ADCC mechanism as well as the macrophage-like accessory adherent cell type(s) required for the induction of humoral antibody synthesis and cell-mediated cytotoxic allograft reactions in vitro.", "contents": "Antibody-dependent cell-mediated cytotoxicity. Comparative ability of murine Peyer's patch and spleen cells to lyse lipopolysaccharide-coated and uncoated erythrocytes. Lymphoid cells from nonsensitized donors can be cytotoxic in vitro for target cells coated with antibody. The antibody can be directed either against antigens which are intrinsic to or experimentally attached to the target cell surface. Killing of target cells by this method has been termed antibody-dependent cell-mediated cytotoxicity (ADCC). In these investigations we compared the ability of Peyer's patch cells and spleen cells from nonsensitized mice to lyse lipoplysaccharide-coated and uncoated target cells by an ADCC mechanism. The results demonstrate that spleen cells, but not Peyer's patch cells, from identical mice can be cytotoxic for lipopolysaccharide-coated chicken erythrocytes (CRBC) in the presence of anti-lipopolysaccharide antibody and uncoated CRBC in the presence of anti-CRBC antibody. Thus, Peyer's patches in mice appear to be deficient in the effector cell type(s) required to kill antibody-coated erythrocyte target cells by an ADCC mechanism as well as the macrophage-like accessory adherent cell type(s) required for the induction of humoral antibody synthesis and cell-mediated cytotoxic allograft reactions in vitro."}
{"id": "PMID:1082426", "title": "Spontaneous bacterial peritonitis induced by intraarterial vasopressin therapy.", "content": "Two patients developed spontaneous bacterial peritonitis after infusions of vasopressin into the superior mesenteric or gastroduodenal arteries for upper gastrointestinal hemorrhage. The peritonitis in these patients differed from the typical picture in which a single aerobic organism is responsible, by the presence of multiple organisms, some of which were anaerobic. These findings suggest that the arterial vasoconstriction decreased the integrity of the intestinal mucosal barrier and permitted the transmural migration of enteric organisms from the lumen of the bowel into the ascites-filled peritoneal cavity.", "contents": "Spontaneous bacterial peritonitis induced by intraarterial vasopressin therapy. Two patients developed spontaneous bacterial peritonitis after infusions of vasopressin into the superior mesenteric or gastroduodenal arteries for upper gastrointestinal hemorrhage. The peritonitis in these patients differed from the typical picture in which a single aerobic organism is responsible, by the presence of multiple organisms, some of which were anaerobic. These findings suggest that the arterial vasoconstriction decreased the integrity of the intestinal mucosal barrier and permitted the transmural migration of enteric organisms from the lumen of the bowel into the ascites-filled peritoneal cavity."}
{"id": "PMID:1082434", "title": "[Treatment of Spastic Gait Disorders Using an \"Electro-Gait-Stimulator (\"Schrittstimulator\") (author's transl)].", "content": "A short historical outline of electric treatment of spasticity is given. A specially developed management with a \"gait-stimulator\" is described. Four muscles of each lower extremity being mainly engaged in walk were electrically stimulated in the physiological sequences according to the normal gait. The used electric impulses were of a duration of 0,25 msec and an intensity up to 700 V. Using such a \"gain-stimulator\" in spastic-paraparetic patients a reduced spasticity has been achieved. Positive effect of this treatment has been mostly pronounced, when the programming of impulses was adjusted to the end of the expected physiological contraction of the corresponding muscles. Physiological and pathological data of the \"Silent period\" is proposed to be mostly involved. The application of impulses in physiological sequences seems to reactivate normal reflex - mechanisms which are disturbed by supraspinal laesion. The results indicate that the electric impulses activates muscle-sensory - organs and that impulses on these organs produce a pace-making function on the spinal cord, which lessens spasticity.", "contents": "[Treatment of Spastic Gait Disorders Using an \"Electro-Gait-Stimulator (\"Schrittstimulator\") (author's transl)]. A short historical outline of electric treatment of spasticity is given. A specially developed management with a \"gait-stimulator\" is described. Four muscles of each lower extremity being mainly engaged in walk were electrically stimulated in the physiological sequences according to the normal gait. The used electric impulses were of a duration of 0,25 msec and an intensity up to 700 V. Using such a \"gain-stimulator\" in spastic-paraparetic patients a reduced spasticity has been achieved. Positive effect of this treatment has been mostly pronounced, when the programming of impulses was adjusted to the end of the expected physiological contraction of the corresponding muscles. Physiological and pathological data of the \"Silent period\" is proposed to be mostly involved. The application of impulses in physiological sequences seems to reactivate normal reflex - mechanisms which are disturbed by supraspinal laesion. The results indicate that the electric impulses activates muscle-sensory - organs and that impulses on these organs produce a pace-making function on the spinal cord, which lessens spasticity."}
{"id": "PMID:1082435", "title": "[Synthesis and biological activity of some cystine peptides from the active center of the basic trypsin inhibitor from bovine organs (Kunitz inhibitor) (author's transl)].", "content": "The synthesis of the following 3 model peptides from the active center of the inhibitor molecule is described: (see article). The 3 peptides are split by trypsin with velocities increasing from (I) to (III). The following Ki values were determined: (I) Ki = 2.3 x 10(-2)M; (II) Ki = 4.9 x 10(-3)M; (III) Ki = 2.1 x 10(-3)M. These Ki values differ from the Ki values of the Kunitz inhibitor by a factor 10(11) to 10(12).", "contents": "[Synthesis and biological activity of some cystine peptides from the active center of the basic trypsin inhibitor from bovine organs (Kunitz inhibitor) (author's transl)]. The synthesis of the following 3 model peptides from the active center of the inhibitor molecule is described: (see article). The 3 peptides are split by trypsin with velocities increasing from (I) to (III). The following Ki values were determined: (I) Ki = 2.3 x 10(-2)M; (II) Ki = 4.9 x 10(-3)M; (III) Ki = 2.1 x 10(-3)M. These Ki values differ from the Ki values of the Kunitz inhibitor by a factor 10(11) to 10(12)."}
{"id": "PMID:1082436", "title": "Cytogenetic and cell-surface marker studies in two non-Hodgkin's lymphomata of T-cell origin.", "content": "The chromosomes from 2 cases of lymphoma involving proliferation of abnormal T-lymphocytes are described. The tumours differed histologically and chromosomally, although certain of the structural chromosome changes found are of common occurrence in other, mostly B-cell, lymphomata.", "contents": "Cytogenetic and cell-surface marker studies in two non-Hodgkin's lymphomata of T-cell origin. The chromosomes from 2 cases of lymphoma involving proliferation of abnormal T-lymphocytes are described. The tumours differed histologically and chromosomally, although certain of the structural chromosome changes found are of common occurrence in other, mostly B-cell, lymphomata."}
{"id": "PMID:1082438", "title": "Evidence against T-cell immunoglobulin from radioimmunoassay on serum and cells from bursectomized chickens.", "content": "A radioimmunoassay with a sensitivity of about 50 pg of chicken immunoglobulin (Ig) was developed and employed for the estimation of Ig in the serum and in extracts of cells from hormonally bursectomized chickens. Chickens, which were judged agammaglobulinaemic by rocket immunoelectrophoresis, had at 12 weeks of age 5-100 ng of Ig/ml of serum. The Ig concentration was decreasing with a half life of 5 days. Thymus and spleen cells from such hypogammaglobulinaemic chickens were extracted with non-ionic detergents, acid urea, or combinations of urea and detergent, and the extracts were analysed for Ig by the inhibition assay. The amount of Ig found in extracts from thymocytes corresponded to less than a hundred molecules of 7S Ig/cell with a lower value of less than five molecules/cell, while extracts of spleen cells contained Ig corresponding to 60-300 molecules of Ig/cell. The inclusion in the media of a number of protease inhibitors did not increase the recovery. Extracts of thymus and spleen cells from untreated chickens contained Ig corresponding to 10-20 X 10(3) and 50-150 X 10(3) molecules of Ig/cell respectively. The results demonstrate the profound hypogammaglobulinaemia of bursectomized chickens and question the concept of an active production of receptor Ig by bursa-independent T cells.", "contents": "Evidence against T-cell immunoglobulin from radioimmunoassay on serum and cells from bursectomized chickens. A radioimmunoassay with a sensitivity of about 50 pg of chicken immunoglobulin (Ig) was developed and employed for the estimation of Ig in the serum and in extracts of cells from hormonally bursectomized chickens. Chickens, which were judged agammaglobulinaemic by rocket immunoelectrophoresis, had at 12 weeks of age 5-100 ng of Ig/ml of serum. The Ig concentration was decreasing with a half life of 5 days. Thymus and spleen cells from such hypogammaglobulinaemic chickens were extracted with non-ionic detergents, acid urea, or combinations of urea and detergent, and the extracts were analysed for Ig by the inhibition assay. The amount of Ig found in extracts from thymocytes corresponded to less than a hundred molecules of 7S Ig/cell with a lower value of less than five molecules/cell, while extracts of spleen cells contained Ig corresponding to 60-300 molecules of Ig/cell. The inclusion in the media of a number of protease inhibitors did not increase the recovery. Extracts of thymus and spleen cells from untreated chickens contained Ig corresponding to 10-20 X 10(3) and 50-150 X 10(3) molecules of Ig/cell respectively. The results demonstrate the profound hypogammaglobulinaemia of bursectomized chickens and question the concept of an active production of receptor Ig by bursa-independent T cells."}
{"id": "PMID:1082439", "title": "Requirement for T cells in the antibody response of mice to calf skin collagen.", "content": "The antibody response to triple-helical calf skin collagen is apparently regulated by T cells since nude mice (BALB/c strain) responded to this antigen only after transfer of T cells. Syngeneic transfer of B and T cells was required to prepare thymectomized and irradiated normal C57B1/10 recipients for an anti-collagen response. Reconstitution by B cells alone was unsuccessful. The results are compatible with previous evidence on the H-2 linked genetic control of the antibody response to and the non-repetitious nature of helical antigenic determinants in calf collagen.", "contents": "Requirement for T cells in the antibody response of mice to calf skin collagen. The antibody response to triple-helical calf skin collagen is apparently regulated by T cells since nude mice (BALB/c strain) responded to this antigen only after transfer of T cells. Syngeneic transfer of B and T cells was required to prepare thymectomized and irradiated normal C57B1/10 recipients for an anti-collagen response. Reconstitution by B cells alone was unsuccessful. The results are compatible with previous evidence on the H-2 linked genetic control of the antibody response to and the non-repetitious nature of helical antigenic determinants in calf collagen."}
{"id": "PMID:1082442", "title": "Lymphocyte response to T-cell mitogen during experimental gingivitis in humans.", "content": "This study was conducted to evaluate the dose response relationships of peripheral blood lymphocytes (PBL) by stimulation of phytohemagglutinin (PHA) during the onset of oral inflammation. Eleven dental students underwent a 3-week experimental gingivitis program (L\u00f6e et al., 1965). At time zero, weeks 1, 2, and 3, and after 1 week of reinstituted oral hygiene (week 4), the plaque accumulations were evaluated, the degree of gingival inflammation was assessed, and a blood sample was taken. Quadruplicate microcultures each containing 2 x 10(5) PBL in 0.2 ml of tissue culture medium 199 and 10% fetal calf serum were stimulated with five concentrations of PHA (10 to 0.5 mug/ml) and incubated for 78 h at 37 C in 5% CO2. [3H]thymidine was added to each culture for the final 8 h. The cultures were then harvested and counted by liquid scintillation, and stimulation indexes (SI) were determined. At time zero the maximum PBL response occurred at a PHA concentration of 5 mug/ml (SI = 100). During weeks 1, 2, and 3 the location of the maximum PBL response shifted to a lower PHA concentration (1.0 mug/ml) and increased to over SI =400. The phenomenon of shifting peak PHA responses to lower PHA concentrations could be observed after only 1 week of developing gingival inflammation. The PBL response returned to pre-experimental values after 1 week of reinstituted oral hygiene, which resolved the oral inflammation. The findings show that a dose response relationship exists between PHA concentrations and the PBL response. If these dose response changes seen during developing gingival inflammation are ignored, either a decrease, increase, or no change in PBL response can be shown depending upon the PHA concentration evaluated. Owing to the dose-dependent nature of this PBL response, it is advisable to routinely use dose response curves in order to properly evaluate the full responsiveness of PBL to mitogenic substances.", "contents": "Lymphocyte response to T-cell mitogen during experimental gingivitis in humans. This study was conducted to evaluate the dose response relationships of peripheral blood lymphocytes (PBL) by stimulation of phytohemagglutinin (PHA) during the onset of oral inflammation. Eleven dental students underwent a 3-week experimental gingivitis program (L\u00f6e et al., 1965). At time zero, weeks 1, 2, and 3, and after 1 week of reinstituted oral hygiene (week 4), the plaque accumulations were evaluated, the degree of gingival inflammation was assessed, and a blood sample was taken. Quadruplicate microcultures each containing 2 x 10(5) PBL in 0.2 ml of tissue culture medium 199 and 10% fetal calf serum were stimulated with five concentrations of PHA (10 to 0.5 mug/ml) and incubated for 78 h at 37 C in 5% CO2. [3H]thymidine was added to each culture for the final 8 h. The cultures were then harvested and counted by liquid scintillation, and stimulation indexes (SI) were determined. At time zero the maximum PBL response occurred at a PHA concentration of 5 mug/ml (SI = 100). During weeks 1, 2, and 3 the location of the maximum PBL response shifted to a lower PHA concentration (1.0 mug/ml) and increased to over SI =400. The phenomenon of shifting peak PHA responses to lower PHA concentrations could be observed after only 1 week of developing gingival inflammation. The PBL response returned to pre-experimental values after 1 week of reinstituted oral hygiene, which resolved the oral inflammation. The findings show that a dose response relationship exists between PHA concentrations and the PBL response. If these dose response changes seen during developing gingival inflammation are ignored, either a decrease, increase, or no change in PBL response can be shown depending upon the PHA concentration evaluated. Owing to the dose-dependent nature of this PBL response, it is advisable to routinely use dose response curves in order to properly evaluate the full responsiveness of PBL to mitogenic substances."}
{"id": "PMID:1082443", "title": "Cellular immunity in Peyer's patches of rats infected with Trichinella spiralis.", "content": "A rat model of Trichinella spiralis gut infection was used to observe the sequence of developing cellular immunity in Peyer's patches and other lymphoid tissues. Whereas cellular reactivity (lymphocyte blastogenesis) for worm antigens was evident in mesenteric lymph nodes draining the gastrointestinal tract within 3 days after infection, Peyer's patch lymphocytes developed maximal reactivity 2 to 3 weeks later at the same time as the spleen and other lymphoid tissues. Furthermore, the immune reactivity found in Peyer's patches was only transient. Thus, in this parasitic gut infection, the Peyer's patch lymphoid tissue does not appear to be the first site of cellular responsiveness but rather to acquire cellular reactivity only when other lymphoid elements in the infected host have also acquired similar antigen-induced reactivity.", "contents": "Cellular immunity in Peyer's patches of rats infected with Trichinella spiralis. A rat model of Trichinella spiralis gut infection was used to observe the sequence of developing cellular immunity in Peyer's patches and other lymphoid tissues. Whereas cellular reactivity (lymphocyte blastogenesis) for worm antigens was evident in mesenteric lymph nodes draining the gastrointestinal tract within 3 days after infection, Peyer's patch lymphocytes developed maximal reactivity 2 to 3 weeks later at the same time as the spleen and other lymphoid tissues. Furthermore, the immune reactivity found in Peyer's patches was only transient. Thus, in this parasitic gut infection, the Peyer's patch lymphoid tissue does not appear to be the first site of cellular responsiveness but rather to acquire cellular reactivity only when other lymphoid elements in the infected host have also acquired similar antigen-induced reactivity."}
{"id": "PMID:1082444", "title": "T and CR+ lymphocyte profile in leprosy and the effect of treatment.", "content": "Thymus-derived lymphocytes (T lymphocytes) and complement receptor-bearing lymphocytes (CR+ lymphocytes) were estimated by using erythrocyte rosettes and erythrocyte-antibody-complement rosettes as markers in untreated lepromatous and untreated tuberculoid patients and in healthy controls. Treated lepromatous cases were also investigated. Ten cases of untreated lepromatous patients were reassessed 6 months or more after therapy commenced. A significant decrease in both percentages and absolute numbers of CR+ cells in the untreated lepromatous leprosy subjects was observed. This decrease showed a return to normal levels after treatment. The percentage of T cells in the untreated lepromatous cases was normal; however, the absolute numbers of T cells and the total lymphocyte count showed a significant decrease. After therapy, the T cell population was unchanged but the total number of lymphocytes increased significantly with treatment. The absolute number of T and CR+ cells was significantly less in the untreated than in the treated lepromatous patients.", "contents": "T and CR+ lymphocyte profile in leprosy and the effect of treatment. Thymus-derived lymphocytes (T lymphocytes) and complement receptor-bearing lymphocytes (CR+ lymphocytes) were estimated by using erythrocyte rosettes and erythrocyte-antibody-complement rosettes as markers in untreated lepromatous and untreated tuberculoid patients and in healthy controls. Treated lepromatous cases were also investigated. Ten cases of untreated lepromatous patients were reassessed 6 months or more after therapy commenced. A significant decrease in both percentages and absolute numbers of CR+ cells in the untreated lepromatous leprosy subjects was observed. This decrease showed a return to normal levels after treatment. The percentage of T cells in the untreated lepromatous cases was normal; however, the absolute numbers of T cells and the total lymphocyte count showed a significant decrease. After therapy, the T cell population was unchanged but the total number of lymphocytes increased significantly with treatment. The absolute number of T and CR+ cells was significantly less in the untreated than in the treated lepromatous patients."}
{"id": "PMID:1082445", "title": "Effect of viral and bacterial pneumonias on cell-mediated immunity in humans.", "content": "Cell-mediated immunity (CMI) was assessed during infection and after convalescence in 12 patients with influenza pneumonia and 10 patients with bacterial pneumonia. The patients with influenza pneumonia had a marked impairment of skin test reactivity, and their lymphocytes showed a diminished response to phytohemagglutinin and streptokinase-streptodornase stimulation in vitro. Suppression of CMI was related to the severity of the pneumonia. Patients with bacterial pneumonia showed as great a suppression of the response to phytohemagglutinin and streptokinase-streptodornase as the patients with viral pneumonia. All parameters of CMI returned to normal in both groups after convalescence. The depression of CMI could not be related to a decrease in the number of thymus-derived lymphocytes or to serum-suppressive factors in these patients.", "contents": "Effect of viral and bacterial pneumonias on cell-mediated immunity in humans. Cell-mediated immunity (CMI) was assessed during infection and after convalescence in 12 patients with influenza pneumonia and 10 patients with bacterial pneumonia. The patients with influenza pneumonia had a marked impairment of skin test reactivity, and their lymphocytes showed a diminished response to phytohemagglutinin and streptokinase-streptodornase stimulation in vitro. Suppression of CMI was related to the severity of the pneumonia. Patients with bacterial pneumonia showed as great a suppression of the response to phytohemagglutinin and streptokinase-streptodornase as the patients with viral pneumonia. All parameters of CMI returned to normal in both groups after convalescence. The depression of CMI could not be related to a decrease in the number of thymus-derived lymphocytes or to serum-suppressive factors in these patients."}
{"id": "PMID:1082446", "title": "Quantitation of B and T lymphocytes in guinea pigs with evidence for a release of both cell types from the spleen into the blood.", "content": "B and T lymphocytes were quantitated in lymphoid organs and in blood of young normal guinea pigs by the use of EAC rosettes and rabbit erythrocyte (RE) rosettes as markers. Special attention was focused on the release of B and T cells from the spleen, estimated from the difference between the content of B and T cells in splenic efferent and afferent blood. The following frequencies of resette-forming cells (RFC) were found. Thymus: 0.3% EAC- and 88% RE-RFC; spleen: 44% EAC- and 46% RE-RFC; lymph nodes: 13% EAC- and 39% RE-RFC; arterial blood: 13% EAC- and 42% RE-RFC, and bone marrow: 2% EAC- and 7% RE-RFC. A fairly large number of cells in the lymph nodes and blood could not be identified by any of the two markers. Some possible explanations for this are discussed. The content of both EAC- and RE-RFC in splenic efferent blood significantly exceeded that in the afferent blood, indicating a release of both B and T cells from the spleen into the blood. The possibility of a release of a third type of mononuclear cell cannot be excluded from the present results.", "contents": "Quantitation of B and T lymphocytes in guinea pigs with evidence for a release of both cell types from the spleen into the blood. B and T lymphocytes were quantitated in lymphoid organs and in blood of young normal guinea pigs by the use of EAC rosettes and rabbit erythrocyte (RE) rosettes as markers. Special attention was focused on the release of B and T cells from the spleen, estimated from the difference between the content of B and T cells in splenic efferent and afferent blood. The following frequencies of resette-forming cells (RFC) were found. Thymus: 0.3% EAC- and 88% RE-RFC; spleen: 44% EAC- and 46% RE-RFC; lymph nodes: 13% EAC- and 39% RE-RFC; arterial blood: 13% EAC- and 42% RE-RFC, and bone marrow: 2% EAC- and 7% RE-RFC. A fairly large number of cells in the lymph nodes and blood could not be identified by any of the two markers. Some possible explanations for this are discussed. The content of both EAC- and RE-RFC in splenic efferent blood significantly exceeded that in the afferent blood, indicating a release of both B and T cells from the spleen into the blood. The possibility of a release of a third type of mononuclear cell cannot be excluded from the present results."}
{"id": "PMID:1082447", "title": "A case of unusual SLE related syndrome characterized by erythema multiforme, angioneurotic edema, marked hypocomplementemia, and Clq precipitins of the low molecular weight type.", "content": "Our patient and those of Agnello et al. had identical clinical symptoms such as erythema multiforme, arthralgias and angioneurotic edema and both differed from systemic lupus erthematosus in several important points, i.e., in spite of marked hypocomplementemia the nephropathy is not prominent and it needs high-dose steroids to eliminate the clinical and serological abnormalities. In addition to the features reported by Agnello et al. we found increased viral antibody titers in our patient's sera.", "contents": "A case of unusual SLE related syndrome characterized by erythema multiforme, angioneurotic edema, marked hypocomplementemia, and Clq precipitins of the low molecular weight type. Our patient and those of Agnello et al. had identical clinical symptoms such as erythema multiforme, arthralgias and angioneurotic edema and both differed from systemic lupus erthematosus in several important points, i.e., in spite of marked hypocomplementemia the nephropathy is not prominent and it needs high-dose steroids to eliminate the clinical and serological abnormalities. In addition to the features reported by Agnello et al. we found increased viral antibody titers in our patient's sera."}
{"id": "PMID:1082448", "title": "Different surface glycoprotein patterns on human T-, B- and leukemic-lymphocytes.", "content": "We have labelled the exposed surface glycoproteins of human blood T- and B-lymphocytes and cells from patients with chronic lymphocytic leukemia by the galactose oxidase-tritiated sodium borohydride method. The labelled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography. The T- and B-lymphocytes show different and characteristic surface glycoprotein patterns. The surface glycoprotein patterns of the leukemic cells differ from those of normal, non-malignant lymphocytes. A relationship between the altered surface glycoprotein pattern of leukemic cells and the expression of leukemia-associated antigens is discussed.", "contents": "Different surface glycoprotein patterns on human T-, B- and leukemic-lymphocytes. We have labelled the exposed surface glycoproteins of human blood T- and B-lymphocytes and cells from patients with chronic lymphocytic leukemia by the galactose oxidase-tritiated sodium borohydride method. The labelled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography. The T- and B-lymphocytes show different and characteristic surface glycoprotein patterns. The surface glycoprotein patterns of the leukemic cells differ from those of normal, non-malignant lymphocytes. A relationship between the altered surface glycoprotein pattern of leukemic cells and the expression of leukemia-associated antigens is discussed."}
{"id": "PMID:1082449", "title": "Non-T-cell resistance against a mouse Moloney lymphoma.", "content": "We have previously shown that spleen cells from normal mice contain lymphocytes that kill certain in vitro-grown Moloney lymphoma lines in a 51Cr release test. The killing activity shows a marked dependence on the genotype of the donor mouse. In vivo rejection studies with a Moloney lymphoma line in various normal semisyngeneic F1 hybrids showed a clear positive correlation between in vitro natural cytotoxicity and in vivo rejection. Thus mice can be grouped as high- or low-reactive according to their in vitro cytolytic behavior as well as their in vivo rejection potential. A lymphoid cell without detectable T- or B-cell markers is responsible for the in vitro killing effect. The present study also shows that the in vivo growth inhibitory function is T-cell-independent. Lymphoid cells depleted of T- and B-cells and transferred together  with Moloney lymphoma cells into an irradiated syngeneic recipent were highly efficient in delaying tumor growth. Furthermore, syngeneic or semisyngeneic thymecto-mized, irradiated, fetal-liver-reconstituted mice showed if anything an increased in vivo resistance to a Moloney lymphoma compared to control mice. In contrast, tumor cells histoincompatible with regard to the H-2 locus showed the expected preferential growth in the thymectomized animals. We thus conclude that the major in vivo resistance against transplantation of the syngeneic or semisyngeneic Moloney lymphoma used in this study is T-independent.", "contents": "Non-T-cell resistance against a mouse Moloney lymphoma. We have previously shown that spleen cells from normal mice contain lymphocytes that kill certain in vitro-grown Moloney lymphoma lines in a 51Cr release test. The killing activity shows a marked dependence on the genotype of the donor mouse. In vivo rejection studies with a Moloney lymphoma line in various normal semisyngeneic F1 hybrids showed a clear positive correlation between in vitro natural cytotoxicity and in vivo rejection. Thus mice can be grouped as high- or low-reactive according to their in vitro cytolytic behavior as well as their in vivo rejection potential. A lymphoid cell without detectable T- or B-cell markers is responsible for the in vitro killing effect. The present study also shows that the in vivo growth inhibitory function is T-cell-independent. Lymphoid cells depleted of T- and B-cells and transferred together  with Moloney lymphoma cells into an irradiated syngeneic recipent were highly efficient in delaying tumor growth. Furthermore, syngeneic or semisyngeneic thymecto-mized, irradiated, fetal-liver-reconstituted mice showed if anything an increased in vivo resistance to a Moloney lymphoma compared to control mice. In contrast, tumor cells histoincompatible with regard to the H-2 locus showed the expected preferential growth in the thymectomized animals. We thus conclude that the major in vivo resistance against transplantation of the syngeneic or semisyngeneic Moloney lymphoma used in this study is T-independent."}
{"id": "PMID:1082451", "title": "Association of human alpha1-antitrypsin with anhydrotrypsin.", "content": "Unlike in the reaction of anhydrotrypsin and soybean trypsin inhibitor, human alpha1-antitrypsin was found not to form a complex with bovine anhydrotrypsin. This was shown using three different methods: electrophoresis, affinity chromatography on an anhydrotrypsin-coupled Sepharose column and equilibrium competitive binding assay. These results indicate the importance of the active site serine of trypsin in formation of a complex with alpha1-antitrypsin.", "contents": "Association of human alpha1-antitrypsin with anhydrotrypsin. Unlike in the reaction of anhydrotrypsin and soybean trypsin inhibitor, human alpha1-antitrypsin was found not to form a complex with bovine anhydrotrypsin. This was shown using three different methods: electrophoresis, affinity chromatography on an anhydrotrypsin-coupled Sepharose column and equilibrium competitive binding assay. These results indicate the importance of the active site serine of trypsin in formation of a complex with alpha1-antitrypsin."}
{"id": "PMID:1082452", "title": "Adenosine deaminase activity in chronic lymphocytic leukemia. Relationship to B- and T-cell subpopulations.", "content": "The level, phenotypes, and isozyme distribution of adenosine deaminase (ADA) were determined in lymphocytes from patients with chronic lymphocytic leukemia (CLL). The ADA level in lymphocytes from patients with untreated CLL was consistently lower than in lymphocytes from normal subjects. No significant differences were found in the phenotype or isozyme distribution. In untreated patients, the ADA level was inversely correlated with the lymphocyte count and the percentage of bursa-equivalent (B) cells. After therapy, a diminution in the lymphocyte count was associated with an increase of ADA activity towards normal levels. The ADA levels were slightly higher in the thymus-derived (T) than in the B lymphocytes from normal subjects. The B cells had lower activity than T cells in patients with CLL. They also had a lower activity than the B cells from normal subjects. The ADA level was 2.3-fold higher in T cells from patients with CLL than in normal T cells. The decrease in ADA levels is an anomaly that is reversible and appears to be a reflection of the proliferation of abnormal B cells in this disorder.", "contents": "Adenosine deaminase activity in chronic lymphocytic leukemia. Relationship to B- and T-cell subpopulations. The level, phenotypes, and isozyme distribution of adenosine deaminase (ADA) were determined in lymphocytes from patients with chronic lymphocytic leukemia (CLL). The ADA level in lymphocytes from patients with untreated CLL was consistently lower than in lymphocytes from normal subjects. No significant differences were found in the phenotype or isozyme distribution. In untreated patients, the ADA level was inversely correlated with the lymphocyte count and the percentage of bursa-equivalent (B) cells. After therapy, a diminution in the lymphocyte count was associated with an increase of ADA activity towards normal levels. The ADA levels were slightly higher in the thymus-derived (T) than in the B lymphocytes from normal subjects. The B cells had lower activity than T cells in patients with CLL. They also had a lower activity than the B cells from normal subjects. The ADA level was 2.3-fold higher in T cells from patients with CLL than in normal T cells. The decrease in ADA levels is an anomaly that is reversible and appears to be a reflection of the proliferation of abnormal B cells in this disorder."}
{"id": "PMID:1082453", "title": "Spontaneous cytotoxicity (SCMC) of normal human lymphocytes against a human melanoma cell line: a phenomenon due to a lymphotoxin-like mediator.", "content": "Spontaneous cell-mediated cytotoxicity (SCMC) of normal human lymphocytes against various allogenic tumor cell lines has recently been identified as a non-T lymphocyte function. In this study evidence is presented that SCMC against a human melanoma cell line (IGR3) involves a nonspecific lymphotoxin-like mediator(s) (LT), which is rapidly produced by lymphocytes that are retained on IgG-anti-IgG columns. LT was shown to inhibit in 48-hr cultures the DNA synthesis of growing IGR3 and HeLa cell monolayers. Furthermore, in short term 51Cr-release assays using IGR3 target cells, LT increased strongly the SCMC of normal allogeneic lymphocytes, although it exhibited little cytotoxicity by itself in this assay. LT was detectable in cell-free supernatants harvested after 6 hr from co-cultures of IGR3 melanoma cells and normal effector lymphocytes, but not in supernatants from melanoma cells alone or lymphocytes alone. Lymphocyte preparations that had been passed through IgG-anti-IgG columns had lost the capacity to generate LT and were poor effectors in SCMC. However, in the presence of LT, a small proportion of null cells, which pass through IgG-anti-IgG columns, was capable of inducing strong SCMC. Absorption of the supernatants, containing LT activity, with an insolubilized rabbit-anti-human IgG antiserum did not remove the mediator, suggesting that it is not an immunoglobulin.", "contents": "Spontaneous cytotoxicity (SCMC) of normal human lymphocytes against a human melanoma cell line: a phenomenon due to a lymphotoxin-like mediator. Spontaneous cell-mediated cytotoxicity (SCMC) of normal human lymphocytes against various allogenic tumor cell lines has recently been identified as a non-T lymphocyte function. In this study evidence is presented that SCMC against a human melanoma cell line (IGR3) involves a nonspecific lymphotoxin-like mediator(s) (LT), which is rapidly produced by lymphocytes that are retained on IgG-anti-IgG columns. LT was shown to inhibit in 48-hr cultures the DNA synthesis of growing IGR3 and HeLa cell monolayers. Furthermore, in short term 51Cr-release assays using IGR3 target cells, LT increased strongly the SCMC of normal allogeneic lymphocytes, although it exhibited little cytotoxicity by itself in this assay. LT was detectable in cell-free supernatants harvested after 6 hr from co-cultures of IGR3 melanoma cells and normal effector lymphocytes, but not in supernatants from melanoma cells alone or lymphocytes alone. Lymphocyte preparations that had been passed through IgG-anti-IgG columns had lost the capacity to generate LT and were poor effectors in SCMC. However, in the presence of LT, a small proportion of null cells, which pass through IgG-anti-IgG columns, was capable of inducing strong SCMC. Absorption of the supernatants, containing LT activity, with an insolubilized rabbit-anti-human IgG antiserum did not remove the mediator, suggesting that it is not an immunoglobulin."}
{"id": "PMID:1082454", "title": "Activation of purified human thymus-derived (T) cells by mitogens. II. Monocyte- macrophage potentiation of mitogen-induced DNA synthesis.", "content": "Thymus-derived (T) cells from peripheral blood were purified by rosette formation with neuraminidase-treated sheep red blood cells (SRBC) and centifugation on Ficoll-Hypaque. T cells recovered from the pellet were freed of SRBC by treatment with Tris-NH4Cl. T cells purified by this method showed a diminished ability to take up 3H-thymidine (3H-TdR) after mitogen stimulation when compared to the mitogenic response of an equal number of autologous peripheral blood mononuclear lymphocytes (PBL). Autologous monocytes restored the capacity of purified T cells to take up 3H-TdR in the presence of phytohemagglutinin (PHA) or Concanavalin A (Con A). The effect was proportional to the number of monocytes added. Similar restorative effects could be obtained with allogeneic or xenogeneic monocytes. These data suggest that the mitogenic stimulation of human PBL and Con A may reflect the participation of more than one cell type: the T cells and monocyte and that the genetic origin of the monocyte is not critical for augmentation of the mitogenic activation of human T cells.", "contents": "Activation of purified human thymus-derived (T) cells by mitogens. II. Monocyte- macrophage potentiation of mitogen-induced DNA synthesis. Thymus-derived (T) cells from peripheral blood were purified by rosette formation with neuraminidase-treated sheep red blood cells (SRBC) and centifugation on Ficoll-Hypaque. T cells recovered from the pellet were freed of SRBC by treatment with Tris-NH4Cl. T cells purified by this method showed a diminished ability to take up 3H-thymidine (3H-TdR) after mitogen stimulation when compared to the mitogenic response of an equal number of autologous peripheral blood mononuclear lymphocytes (PBL). Autologous monocytes restored the capacity of purified T cells to take up 3H-TdR in the presence of phytohemagglutinin (PHA) or Concanavalin A (Con A). The effect was proportional to the number of monocytes added. Similar restorative effects could be obtained with allogeneic or xenogeneic monocytes. These data suggest that the mitogenic stimulation of human PBL and Con A may reflect the participation of more than one cell type: the T cells and monocyte and that the genetic origin of the monocyte is not critical for augmentation of the mitogenic activation of human T cells."}
{"id": "PMID:1082455", "title": "The distribution of Ia antigens on the surfaces of lymphocytes.", "content": "The distribution of Ia antigens was studied on murine spleen lymphocytes by an ultrastructural technique employing deep freeze-etched replicas. Ia antigens were labeled on cells from appropriate congenic and recombinant strains of mice by incubating the cells with FITC-conjugated anti-Iak antibody, followed by ferritin-coupled Fab anti-FITC. Ia antigens were detected predominantly on immunoglobulin (Ig)-bearing B lymphocytes. Antigens coded for by the entire Ik region were present on the surfaces of 95% of the positive cells (from B10.BR mice) in densely packed microclusters. Ia specificities coded for by the I-A and I-C subregions (on 4R and B10.HTT mice) exhibited a more variable pattern, with 30 to 35% of the labeled cells having sparsely distributed Ia antigens in relatively discrete microclusters. Binding of anti-Iak antibody at 37 degrees C led to patch formation but not to capping. Modulation of surface Ig left Ia antigens diffusely distributed on the cell surface, indicating that these two membrane proteins are independent molecules.", "contents": "The distribution of Ia antigens on the surfaces of lymphocytes. The distribution of Ia antigens was studied on murine spleen lymphocytes by an ultrastructural technique employing deep freeze-etched replicas. Ia antigens were labeled on cells from appropriate congenic and recombinant strains of mice by incubating the cells with FITC-conjugated anti-Iak antibody, followed by ferritin-coupled Fab anti-FITC. Ia antigens were detected predominantly on immunoglobulin (Ig)-bearing B lymphocytes. Antigens coded for by the entire Ik region were present on the surfaces of 95% of the positive cells (from B10.BR mice) in densely packed microclusters. Ia specificities coded for by the I-A and I-C subregions (on 4R and B10.HTT mice) exhibited a more variable pattern, with 30 to 35% of the labeled cells having sparsely distributed Ia antigens in relatively discrete microclusters. Binding of anti-Iak antibody at 37 degrees C led to patch formation but not to capping. Modulation of surface Ig left Ia antigens diffusely distributed on the cell surface, indicating that these two membrane proteins are independent molecules."}
{"id": "PMID:1082456", "title": "In vitro generation of secondary cell-mediated cytotoxic response against a syngeneic Gross virus-induced lymphoma in rats.", "content": "Cell-mediated cytotoxic responses against a syngeneic Gross virus-induced lymphoma, (C58NT)D, in W/Fu rats were generated in vitro by using mixed lymphocyte-tumor cell cultures. The source of responding cells was either spleens from normal rats or spleens from rats carrying or having rejected (C58NT)D tumors. Mitomycin C-treated (C58NT)D tumor cells were used as stimulating cells. The secondary anti-tumor cytotoxic response occurred more rapidly and reached higher levels than the primary response, and it was antigen specific. T cells, but nor B cells or macrophages, were essential for both the induction and the effector phases of the secondary anti-tumor responses. These data suggest that specific memory T cells persist for long periods of time in the lymphoid organs of (C58NT)D immune rats, which can rapidly become cytotoxic upon re-exposure to antigen.", "contents": "In vitro generation of secondary cell-mediated cytotoxic response against a syngeneic Gross virus-induced lymphoma in rats. Cell-mediated cytotoxic responses against a syngeneic Gross virus-induced lymphoma, (C58NT)D, in W/Fu rats were generated in vitro by using mixed lymphocyte-tumor cell cultures. The source of responding cells was either spleens from normal rats or spleens from rats carrying or having rejected (C58NT)D tumors. Mitomycin C-treated (C58NT)D tumor cells were used as stimulating cells. The secondary anti-tumor cytotoxic response occurred more rapidly and reached higher levels than the primary response, and it was antigen specific. T cells, but nor B cells or macrophages, were essential for both the induction and the effector phases of the secondary anti-tumor responses. These data suggest that specific memory T cells persist for long periods of time in the lymphoid organs of (C58NT)D immune rats, which can rapidly become cytotoxic upon re-exposure to antigen."}
{"id": "PMID:1082457", "title": "In vitro response of subpopulations of human tonsil lymphocytes. I. Cellular collaboration in the proliferative response to PHA and Con A.", "content": "Human tonsil lymphocytes have been separated into three subpopulations of cells: purified B cells and two subsets of purified T cells (F1 and F2). B cells were obtained by rosetting with neuraminidase treated SRBC. F1 and F2 were separated by filtration on a nylon wool column using different speeds of elution. Purified B cells contained less than 5% T cells, the T cells preparations contained less than 5% B cells for F1 and 10 to 15% for F2, respectively. A significant contamination in cells not identified by any B or T marker was observed in purified B cells and in F1. Adherent cells enhanced the response of each lymphochte population to PHA and Con A. This explained the paradoxically low responsiveness of the purified T cells. Purified B cells did not respond to these mitogens in different culture conditions. However, a small B cell response was observed when they were cultured in the presence of mitomycin-treated T cells. Striking was the enhancing effect of B cells on the T cell response to PHA and Con A. This enhancing effect was observed even when B cells were treated with mitomycin or depleted in adherent cells. The comparison of the F1 and F2 response suggested that they contained distinct types of T cells.", "contents": "In vitro response of subpopulations of human tonsil lymphocytes. I. Cellular collaboration in the proliferative response to PHA and Con A. Human tonsil lymphocytes have been separated into three subpopulations of cells: purified B cells and two subsets of purified T cells (F1 and F2). B cells were obtained by rosetting with neuraminidase treated SRBC. F1 and F2 were separated by filtration on a nylon wool column using different speeds of elution. Purified B cells contained less than 5% T cells, the T cells preparations contained less than 5% B cells for F1 and 10 to 15% for F2, respectively. A significant contamination in cells not identified by any B or T marker was observed in purified B cells and in F1. Adherent cells enhanced the response of each lymphochte population to PHA and Con A. This explained the paradoxically low responsiveness of the purified T cells. Purified B cells did not respond to these mitogens in different culture conditions. However, a small B cell response was observed when they were cultured in the presence of mitomycin-treated T cells. Striking was the enhancing effect of B cells on the T cell response to PHA and Con A. This enhancing effect was observed even when B cells were treated with mitomycin or depleted in adherent cells. The comparison of the F1 and F2 response suggested that they contained distinct types of T cells."}
{"id": "PMID:1082458", "title": "In vitro response of subpopulations of human lymphocytes. II. DNA synthesis induced by anti-immunoglobulin antibodies.", "content": "A significant and constant increase in DNA synthesis was observed in human lymphocytes cultured in the presence of purified anti-immunoglobulin antibodies specific for human IgG, IgA, and IgM. This has been found in cultures of lymphocytes isolated from blood, tonsils, spleen, and lymph nodes. The optimal culture conditions for blood and tonsil lymphocytes were determined. As a rule 6-day cultures containing 2 x 10(6) cells/ml and 100 mug/ml of antibody yielded the highest 3H-thymidine uptake. Purified T cell cultures could not be stimulated, whereas a low response could be observed in most of the purified B cell cultures. Optimal culture conditions were the same for the B and total tonsil lymphocytes. However, when the purified B cells were totally depleted of T cells, no response was observed. A T and  B cell synergy has been demonstrated by supplementing B cell cultures with purified T cells, whether treated or not with mitomycin. These experiments indicated a permissive and potentiating effect of T cells on the B cell response. Cultures containing mitomycin-treated B cells and purified T cells (mB + T) could be stimulated by a-Ig, thus indicating a T cell proliferation. In keeping with this finding was the observation of an increased response of total lymphocytes supplemented with T cells but not with B cells. Adherent cells are necessary for an optimal response to a-Ig; they enhanced the B cell proliferation observed in (Tm + B) cultures and suppressed the response of T cells in (T + Bm) cultures.", "contents": "In vitro response of subpopulations of human lymphocytes. II. DNA synthesis induced by anti-immunoglobulin antibodies. A significant and constant increase in DNA synthesis was observed in human lymphocytes cultured in the presence of purified anti-immunoglobulin antibodies specific for human IgG, IgA, and IgM. This has been found in cultures of lymphocytes isolated from blood, tonsils, spleen, and lymph nodes. The optimal culture conditions for blood and tonsil lymphocytes were determined. As a rule 6-day cultures containing 2 x 10(6) cells/ml and 100 mug/ml of antibody yielded the highest 3H-thymidine uptake. Purified T cell cultures could not be stimulated, whereas a low response could be observed in most of the purified B cell cultures. Optimal culture conditions were the same for the B and total tonsil lymphocytes. However, when the purified B cells were totally depleted of T cells, no response was observed. A T and  B cell synergy has been demonstrated by supplementing B cell cultures with purified T cells, whether treated or not with mitomycin. These experiments indicated a permissive and potentiating effect of T cells on the B cell response. Cultures containing mitomycin-treated B cells and purified T cells (mB + T) could be stimulated by a-Ig, thus indicating a T cell proliferation. In keeping with this finding was the observation of an increased response of total lymphocytes supplemented with T cells but not with B cells. Adherent cells are necessary for an optimal response to a-Ig; they enhanced the B cell proliferation observed in (Tm + B) cultures and suppressed the response of T cells in (T + Bm) cultures."}
{"id": "PMID:1082459", "title": "The role of lymphotoxin in target cell destruction induced by mitogen-activated human lymphocytes in vitro. II. The correlation of temperature and trypsin-sensitive phases of lymphotoxin-induced and lymphocyte-mediated cytotoxicity.", "content": "The in vitro destruction of phytohemagglutinin (PHA) coated Beta L cells by non-immune human lymphocytes was resolved into two distinct phases--lymphocyte dependent and lymphocyte independent. The initial or lymphocyte-dependent phase occurred within the first 2 hr and proceeded equally well at 34 and 37 degrees C. The amount of lymphotoxin (LT) secreted by PHA-activated human lymphocytes in vitro to PHA stimulation was the same at 34 and 37 degrees C. Antiserum and complement inactivation of the aggressor lymphocytes at various intervals revealed that target cell lysis was lymphocyte independent. However, the latter phase was temperature dependent, i.e., proceeding at the permissive temperature of 37 degrees C, but inhibited at the restrictive temperature of 34 degrees C. Further experiments revealed that LT-induced destruction had the same temperature sensitivity as target cell cytolysis occurring during the lymphocyte-independent step. Trypsin treatment of target cells during an early period of the lymphocyte-independent phase protected the target cell from subsequent death, indicating the aggressor lymphocyte has deposited a cytotoxic effector material on its surface. These results suggest the lymphocyte-dependent stage involves the processes required for the induction of LT synthesis and secretion. The actual cytolysis occurring during the lymphocyte-independent stage may be caused by LT or LT-like material(s) deposited on the target cell surface by the mitogen-activated human lymphocyte.", "contents": "The role of lymphotoxin in target cell destruction induced by mitogen-activated human lymphocytes in vitro. II. The correlation of temperature and trypsin-sensitive phases of lymphotoxin-induced and lymphocyte-mediated cytotoxicity. The in vitro destruction of phytohemagglutinin (PHA) coated Beta L cells by non-immune human lymphocytes was resolved into two distinct phases--lymphocyte dependent and lymphocyte independent. The initial or lymphocyte-dependent phase occurred within the first 2 hr and proceeded equally well at 34 and 37 degrees C. The amount of lymphotoxin (LT) secreted by PHA-activated human lymphocytes in vitro to PHA stimulation was the same at 34 and 37 degrees C. Antiserum and complement inactivation of the aggressor lymphocytes at various intervals revealed that target cell lysis was lymphocyte independent. However, the latter phase was temperature dependent, i.e., proceeding at the permissive temperature of 37 degrees C, but inhibited at the restrictive temperature of 34 degrees C. Further experiments revealed that LT-induced destruction had the same temperature sensitivity as target cell cytolysis occurring during the lymphocyte-independent step. Trypsin treatment of target cells during an early period of the lymphocyte-independent phase protected the target cell from subsequent death, indicating the aggressor lymphocyte has deposited a cytotoxic effector material on its surface. These results suggest the lymphocyte-dependent stage involves the processes required for the induction of LT synthesis and secretion. The actual cytolysis occurring during the lymphocyte-independent stage may be caused by LT or LT-like material(s) deposited on the target cell surface by the mitogen-activated human lymphocyte."}
{"id": "PMID:1082460", "title": "Ros\u00e9tte formation between human b lymphocytes and zymosan-C3 complexes using different complement sources.", "content": "Sera from five animal species were studied as complement source in the preparation of zymosan-C3 complexes used to detect human B lymphocytes. There was no significant difference in the determination of the percentage of ZC rosette forming cells due to differences in complement source. Human B lymphocytes could not discriminate C3 from different species studied. Every one of these sources of complement may be used interchangeably without altering the percentage of B cells detected by this method.", "contents": "Ros\u00e9tte formation between human b lymphocytes and zymosan-C3 complexes using different complement sources. Sera from five animal species were studied as complement source in the preparation of zymosan-C3 complexes used to detect human B lymphocytes. There was no significant difference in the determination of the percentage of ZC rosette forming cells due to differences in complement source. Human B lymphocytes could not discriminate C3 from different species studied. Every one of these sources of complement may be used interchangeably without altering the percentage of B cells detected by this method."}
{"id": "PMID:1082461", "title": "Induction of specific anti-guinea pig T cell sera in rabbits.", "content": "In an attempt to increase the specificity of antisera raised in rabbits against strain 2 guinea pig thymocytes and brain, the rabbits were screened for titres of natural antibodies to thymocytes and other lymphocytes. Although unimmunized rabbits commonly had moderate titres of cytotoxic antibodies to guinea pig thymocytes, occasional animals had low titres to thymocytes and moderate titres to bone marrow cells. Intravenous immunization of this latter group of rabbits with thymocytes led to the production of high titred anti-thymocyte sera which were easily made specific for thymus-derived lymphocytes (T cells) by absorption with L2 C lymphoma, a bone marrow-derived lymphoma of strain 2 guinea pigs. Sera raised against guinea pig brain in complete Freund's adjuvant which had high titres of antibodies to both thymocytes and bone marrow cells could be made specific for T cells only with great difficulty. The cytotoxic activity of the anti-T cell serum could be absorbed by strain 2 thymocytes and brain homogenates, while high dilutions of this serum inhibited the formation of spontaneous rosettes between guinea pig lymphoid cells and normal rabbit erythrocytes.", "contents": "Induction of specific anti-guinea pig T cell sera in rabbits. In an attempt to increase the specificity of antisera raised in rabbits against strain 2 guinea pig thymocytes and brain, the rabbits were screened for titres of natural antibodies to thymocytes and other lymphocytes. Although unimmunized rabbits commonly had moderate titres of cytotoxic antibodies to guinea pig thymocytes, occasional animals had low titres to thymocytes and moderate titres to bone marrow cells. Intravenous immunization of this latter group of rabbits with thymocytes led to the production of high titred anti-thymocyte sera which were easily made specific for thymus-derived lymphocytes (T cells) by absorption with L2 C lymphoma, a bone marrow-derived lymphoma of strain 2 guinea pigs. Sera raised against guinea pig brain in complete Freund's adjuvant which had high titres of antibodies to both thymocytes and bone marrow cells could be made specific for T cells only with great difficulty. The cytotoxic activity of the anti-T cell serum could be absorbed by strain 2 thymocytes and brain homogenates, while high dilutions of this serum inhibited the formation of spontaneous rosettes between guinea pig lymphoid cells and normal rabbit erythrocytes."}
{"id": "PMID:1082462", "title": "75SeL-selenomethionine: a new isotopic marker for lymphocyte localisation studies.", "content": "L-selenomethionine (SeM), an analogue of methionine containing Se, is incorporated by mouse and rat lymphocytes in vitro: labelled with 75Se (75SeM) it emits gamma-rays distinguishable in energy from those of 125I and 51Cr. The usefulness of 75SeM for lymphocyte localisation studies was investigated. Both B and T cells incorporated 75SeM. Reutilization of the isotope in the mouse after injection of killed cells or allogeneic cells into immunized recipients was 1-2% of the injected dose. The immunocompetence of labelled mouse T and B cells was assessed in irradiated recipients, no effect of 75SeM incorporation being found. The distribution of labelled lymphocytes from various tissues in syngeneic recipients was found to be comparable to that previously obtained with 51Cr.", "contents": "75SeL-selenomethionine: a new isotopic marker for lymphocyte localisation studies. L-selenomethionine (SeM), an analogue of methionine containing Se, is incorporated by mouse and rat lymphocytes in vitro: labelled with 75Se (75SeM) it emits gamma-rays distinguishable in energy from those of 125I and 51Cr. The usefulness of 75SeM for lymphocyte localisation studies was investigated. Both B and T cells incorporated 75SeM. Reutilization of the isotope in the mouse after injection of killed cells or allogeneic cells into immunized recipients was 1-2% of the injected dose. The immunocompetence of labelled mouse T and B cells was assessed in irradiated recipients, no effect of 75SeM incorporation being found. The distribution of labelled lymphocytes from various tissues in syngeneic recipients was found to be comparable to that previously obtained with 51Cr."}
{"id": "PMID:1082465", "title": "Mechanism of inhibition of adoptive transfer of tuberculin sensitivity in acute uremia.", "content": "Cell-mediated immunity is reduced in uremia, but the mechanism of this inhibition is poorly understood. To determine the site of inhibition a study of adoptive transfer of cell-mediated immunity was undertaken. Strain 13 guinea pigs were made uremic by ureteral ligation. Lymph node cells were isolated and transferred in the following three ways: Group 1: tuberculin sensitive nonuremic donors to nonimmune nonuremic recipients; Group 2: tuberculin sensitive nonuremic donors to nonimmune uremic recipients; Group 3: tuberculin sensitive uremic donors to nonimmune nonuremic recipients. There was no difference in the skin test responses of the transfer recipients in Groups 1 and 3, even though the uremic donors in Group 3 had lost their skin test reactivity. Transfer of tuberculin sensitivity was not successful in Group 2. These results indicate that the uremic state inhibits previously established skin test reactivity but does not prevent sensitized T lymphocytes from transferring cellular immunity.", "contents": "Mechanism of inhibition of adoptive transfer of tuberculin sensitivity in acute uremia. Cell-mediated immunity is reduced in uremia, but the mechanism of this inhibition is poorly understood. To determine the site of inhibition a study of adoptive transfer of cell-mediated immunity was undertaken. Strain 13 guinea pigs were made uremic by ureteral ligation. Lymph node cells were isolated and transferred in the following three ways: Group 1: tuberculin sensitive nonuremic donors to nonimmune nonuremic recipients; Group 2: tuberculin sensitive nonuremic donors to nonimmune uremic recipients; Group 3: tuberculin sensitive uremic donors to nonimmune nonuremic recipients. There was no difference in the skin test responses of the transfer recipients in Groups 1 and 3, even though the uremic donors in Group 3 had lost their skin test reactivity. Transfer of tuberculin sensitivity was not successful in Group 2. These results indicate that the uremic state inhibits previously established skin test reactivity but does not prevent sensitized T lymphocytes from transferring cellular immunity."}
{"id": "PMID:1082467", "title": "Otosclerotic obliteration of oval window niche: an analysis of the results of surgery.", "content": "1. The massive otosclerotic focus, obliterating the oval window niche, has a relatively high case incidence of 11-2 per cent in South Australia. The three classes of obliterated footplate are defined. 2. Basic data of 109 consecutive surgical cases of obliterative otosclerosis are given. A brief description of operative techniques--vein graft and polyethylene tube in nine, and piston technique in 100--is given and the difficulties and complications arising at or after surgery are discussed. 3. Post-operative follow-up of patients with yearly audiometric assessment has been achieved in 105/109 (= 96-33%) at four years, and in 94/109 (= 86-26%) at five years. 4. The results of surgery are presented by many and varied methods including bar diagrams, post-operative hearing gains, post-operative bone-air gaps, speech discrimination studies and standard statistical analysis techniques. 5. The vein graft technique (nine cases) is very much inferior to the piston technique. Vein graft cases had a 33% incidence of profound sensori-neural loss due to bony reclosure of the window. 6. The piston technique (100 cases) gave hearing gains of 20 decibels or more in 91%, 30 decibels or more in 71%, and 40 decibels or more in 41% of cases at five years post-operatively. The bone-air gap at five years post-operatively was diminished to 20 decibels or less in 88%, to 15 decibels or less in 85%, to 10 decibels or less in 77%, and complete closure or over-closure occurred in 52% of the patients. 7. Data analysis has established there is no change with time of the post-operative mean bone-air gap 500--2,000 Hz. over a period of at least five years, and thus that the piston operation gives a persisting and stable hearing result. 8. In predicting the effect of the piston operation only a rough guide can be obtained from other variables. In particular bone hardness, mucosal characteristics, sex and piston diameter appear to be irrelevant. Patients with a large bone conduction reading or a large air-bone gap appear to do slightly worse than patients with smaller values for these variables. There are no grounds for excluding a patient from operation on account of age, or the finding of active otosclerosis (soft bone and thickened vascular muco-periosteum). 9. From the data of piston operations, the estimated mean bone-air gap for the five-year examination period was 4-14 db. with a standard deviation of 12-5 db. 10. The piston operation is highly recommended as a safe and suitable method of alleviating, in the long-term, the conductive hearing loss of patients with obliterative otosclerosis of the oval window.", "contents": "Otosclerotic obliteration of oval window niche: an analysis of the results of surgery. 1. The massive otosclerotic focus, obliterating the oval window niche, has a relatively high case incidence of 11-2 per cent in South Australia. The three classes of obliterated footplate are defined. 2. Basic data of 109 consecutive surgical cases of obliterative otosclerosis are given. A brief description of operative techniques--vein graft and polyethylene tube in nine, and piston technique in 100--is given and the difficulties and complications arising at or after surgery are discussed. 3. Post-operative follow-up of patients with yearly audiometric assessment has been achieved in 105/109 (= 96-33%) at four years, and in 94/109 (= 86-26%) at five years. 4. The results of surgery are presented by many and varied methods including bar diagrams, post-operative hearing gains, post-operative bone-air gaps, speech discrimination studies and standard statistical analysis techniques. 5. The vein graft technique (nine cases) is very much inferior to the piston technique. Vein graft cases had a 33% incidence of profound sensori-neural loss due to bony reclosure of the window. 6. The piston technique (100 cases) gave hearing gains of 20 decibels or more in 91%, 30 decibels or more in 71%, and 40 decibels or more in 41% of cases at five years post-operatively. The bone-air gap at five years post-operatively was diminished to 20 decibels or less in 88%, to 15 decibels or less in 85%, to 10 decibels or less in 77%, and complete closure or over-closure occurred in 52% of the patients. 7. Data analysis has established there is no change with time of the post-operative mean bone-air gap 500--2,000 Hz. over a period of at least five years, and thus that the piston operation gives a persisting and stable hearing result. 8. In predicting the effect of the piston operation only a rough guide can be obtained from other variables. In particular bone hardness, mucosal characteristics, sex and piston diameter appear to be irrelevant. Patients with a large bone conduction reading or a large air-bone gap appear to do slightly worse than patients with smaller values for these variables. There are no grounds for excluding a patient from operation on account of age, or the finding of active otosclerosis (soft bone and thickened vascular muco-periosteum). 9. From the data of piston operations, the estimated mean bone-air gap for the five-year examination period was 4-14 db. with a standard deviation of 12-5 db. 10. The piston operation is highly recommended as a safe and suitable method of alleviating, in the long-term, the conductive hearing loss of patients with obliterative otosclerosis of the oval window."}
{"id": "PMID:1082468", "title": "Histopathological findings in the inner ear caused by measles.", "content": "An otopathological analysis of three cases of viral labyrinthitis was performed. Six temporal bones cut in serial sections were available for this study. According to the degree of degenerative changes in various parts of the inner ear two types of morphologically distinct labyrinthitis after measles are presented: the first one with the port of entrance through the internal auditory meatus and characterized by, first and most significantly, changes in the spiral ganglion cells; and the second, as described previously by Lindsay, with the port of entrance of the virus in the inner ear through the stria vascularis, with degenerative changes in various structures within the endolymphatic duct.", "contents": "Histopathological findings in the inner ear caused by measles. An otopathological analysis of three cases of viral labyrinthitis was performed. Six temporal bones cut in serial sections were available for this study. According to the degree of degenerative changes in various parts of the inner ear two types of morphologically distinct labyrinthitis after measles are presented: the first one with the port of entrance through the internal auditory meatus and characterized by, first and most significantly, changes in the spiral ganglion cells; and the second, as described previously by Lindsay, with the port of entrance of the virus in the inner ear through the stria vascularis, with degenerative changes in various structures within the endolymphatic duct."}
{"id": "PMID:1082485", "title": "Very high tension with very little ATP breakdown by active skeletal muscle.", "content": "The tension developed and the chemical change occurring during stretching at velocities up to 2l0 sec-1 and during isometric contraction of dinitrofluorobenzene-treated and untreated frog sartorius muscle have been measured. At all velocities of stretching the tension was greater than during shortening at the same velocity and the mean rate of chemical change was less than or equal to the rates found in similar studies of shortening. For velocities 0.18, 0.33 and 0.66l0 sec-1, the mean rate of chemical change was significantly less (P less than 0.05) than during shortening at the same velocity. At the highest velocity of stretching the tension at l0 was about 0.8 P0 and the mean rate of chemical change was about the same as that for isometric contraction. At low velocity (0.1 l0 sec-1) the tension was as high as 1.4 P0 and the mean rate of chemical change during the stretches at the three slowest velocities was significantly less (P less than 0.05) than during isometric contraction; the lowest mean rate of chemical change amounted to only about 25% of that for isometric contraction. The fact that the chemical change was so low suggests that during stretching crossbridges can form links and develop tension without breaking down ATP.", "contents": "Very high tension with very little ATP breakdown by active skeletal muscle. The tension developed and the chemical change occurring during stretching at velocities up to 2l0 sec-1 and during isometric contraction of dinitrofluorobenzene-treated and untreated frog sartorius muscle have been measured. At all velocities of stretching the tension was greater than during shortening at the same velocity and the mean rate of chemical change was less than or equal to the rates found in similar studies of shortening. For velocities 0.18, 0.33 and 0.66l0 sec-1, the mean rate of chemical change was significantly less (P less than 0.05) than during shortening at the same velocity. At the highest velocity of stretching the tension at l0 was about 0.8 P0 and the mean rate of chemical change was about the same as that for isometric contraction. At low velocity (0.1 l0 sec-1) the tension was as high as 1.4 P0 and the mean rate of chemical change during the stretches at the three slowest velocities was significantly less (P less than 0.05) than during isometric contraction; the lowest mean rate of chemical change amounted to only about 25% of that for isometric contraction. The fact that the chemical change was so low suggests that during stretching crossbridges can form links and develop tension without breaking down ATP."}
{"id": "PMID:1082486", "title": "Mechanical stresses and morphological patterns in amphibian embryos.", "content": "1. Embryos of Rana temporaria have been dissected and shape alterations of different parts of the embryo, taking place within 1 h of separation, have been studied. Two categories of deformation have been revealed. 2. The first category comprises those deformations which take place immediately after separation. They are insensitive to cooling, cyanide and Cytochalasin B treatment. These deformations, which consist of a shortening of initially elongated cells, are considered to be the passive relaxations of previously established elastic tensil stresses. 3. Deformations of the second category proceed more slowly. They are inhibited by cooling, cyanide and Cytochalasin B treatment, are accompanied by elongation and migration of cells and occasionally lead to rather complex morphodifferentiations of isolated fragments. These processes are considered to be the result of the active work of intracellular contractile systems, either pre-existing or induced de novo. 4. By analysing the arrangement of the passive deformations we have constructed maps of mechanical stresses in embryos from late blastula up to the early tail-bud stage. At several embryonic stages drastic transformations of the stress pattern occur, these transformations being separated by periods during which the pattern of stress distribution remains topologically constant. 5. A correlation between the arrangement of stress lines and the presumptive morphological pattern of the embryo is pointed out. 6. Some possible relations between tensile tissue stresses and active mechanochemical processes are discussed.", "contents": "Mechanical stresses and morphological patterns in amphibian embryos. 1. Embryos of Rana temporaria have been dissected and shape alterations of different parts of the embryo, taking place within 1 h of separation, have been studied. Two categories of deformation have been revealed. 2. The first category comprises those deformations which take place immediately after separation. They are insensitive to cooling, cyanide and Cytochalasin B treatment. These deformations, which consist of a shortening of initially elongated cells, are considered to be the passive relaxations of previously established elastic tensil stresses. 3. Deformations of the second category proceed more slowly. They are inhibited by cooling, cyanide and Cytochalasin B treatment, are accompanied by elongation and migration of cells and occasionally lead to rather complex morphodifferentiations of isolated fragments. These processes are considered to be the result of the active work of intracellular contractile systems, either pre-existing or induced de novo. 4. By analysing the arrangement of the passive deformations we have constructed maps of mechanical stresses in embryos from late blastula up to the early tail-bud stage. At several embryonic stages drastic transformations of the stress pattern occur, these transformations being separated by periods during which the pattern of stress distribution remains topologically constant. 5. A correlation between the arrangement of stress lines and the presumptive morphological pattern of the embryo is pointed out. 6. Some possible relations between tensile tissue stresses and active mechanochemical processes are discussed."}
{"id": "PMID:1082487", "title": "The effect of ablation of the olfactory pits on the development of the habenular nuclei in Rana pipiens.", "content": "The habenular nuclei in the diencephalon of the frog, Rana pipiens, are asymmetrical structures: two discrete cell groups develop on the left side (as medial and lateral nuclei), while a single nucleus is formed on the right side. Experimental animals were subjected to bilateral removal of the olfactory pits at an early embryonic stage, and were maintained with normal control animals until metamorphosis was complete. The length, relative volume and cell number for each of the three nuclei were determined in the control and experimental animals at regular intervals during larval development. In the control animals, the left medial nucleus developed similarly to the right nucleus spatially and temporally; however, the left lateral nucleus was significantly different in its development in the three parameters measured. In the experimental animals the left medial and the right habenular nuclei were alone affected by the removal of the olfactory pits. The results provide experimental evidence that the right and left medial, but not the left lateral, habenular nuclei are centers receiving afferent olfactory fibers.", "contents": "The effect of ablation of the olfactory pits on the development of the habenular nuclei in Rana pipiens. The habenular nuclei in the diencephalon of the frog, Rana pipiens, are asymmetrical structures: two discrete cell groups develop on the left side (as medial and lateral nuclei), while a single nucleus is formed on the right side. Experimental animals were subjected to bilateral removal of the olfactory pits at an early embryonic stage, and were maintained with normal control animals until metamorphosis was complete. The length, relative volume and cell number for each of the three nuclei were determined in the control and experimental animals at regular intervals during larval development. In the control animals, the left medial nucleus developed similarly to the right nucleus spatially and temporally; however, the left lateral nucleus was significantly different in its development in the three parameters measured. In the experimental animals the left medial and the right habenular nuclei were alone affected by the removal of the olfactory pits. The results provide experimental evidence that the right and left medial, but not the left lateral, habenular nuclei are centers receiving afferent olfactory fibers."}
{"id": "PMID:1082488", "title": "Binding of components of the properdin system to cultured human lymphoblastoid cells and B lymphocytes.", "content": "Immunofluorescence studies showed that properdin (P) and factor B bind to C3-C3b receptor bearing human lymphoblastoid cells (Raji, Daudi) and B type human peripheral lymphocytes (HPL). P bound to Raji cells first incubated with normal human serum (NHS). EDTA, but not EGTA, halted the binding of P to cells incubated with NHS. However, fixation of P to Raji cells, after incubation with NHS first reacted with inulin, was independent of Ca++ and -g++ ions. Fixation of P to Raji cells depended on the presence of C3 or C3b and occurred in the absence of factor D and factor B. Binding of P to B type HPL was detectable only after incubation of these cells with NHS first reacted with inulin; under these conditions binding of P to Raji cells was also greatly enhanced. With both Raji cells and HPL, factor B was detectable on cell surfaces only after incubation of these cells with NHS first reacted with activators of the P system. Binding of factor B to cells required the presence of C3b and binding or stabilization of cell bound factor B necessitated the presence of activated P. P and factor B were detectable only on cultured cells having C3-C3b receptors. However, incubation of NHS with all lymphoblastoid cell lines studied resulted in activation of P and cleavage of factor B. Binding of P and factor B to cells may follow one of three sequences; (a) activated P in fluid phase combines with C3, factor D, and factor B, and the whole complex fixes to cellular C3-C3b receptors via its C3 moiety; (b) C3b generated in fluid phase combines with P, C3, factor D, and factor B and binds to C3-C3b receptors; or (c) C3 or C3b first binds onto the C3-C3b receptors and thereafter interacts with P, factor D, And factor B. Binding of components of the P system to cells or other particles may relate to such biological phenomena as lysis, phagocytosis, proliferation, attraction of other cell types, and alteration of responsiveness to external stimuli.", "contents": "Binding of components of the properdin system to cultured human lymphoblastoid cells and B lymphocytes. Immunofluorescence studies showed that properdin (P) and factor B bind to C3-C3b receptor bearing human lymphoblastoid cells (Raji, Daudi) and B type human peripheral lymphocytes (HPL). P bound to Raji cells first incubated with normal human serum (NHS). EDTA, but not EGTA, halted the binding of P to cells incubated with NHS. However, fixation of P to Raji cells, after incubation with NHS first reacted with inulin, was independent of Ca++ and -g++ ions. Fixation of P to Raji cells depended on the presence of C3 or C3b and occurred in the absence of factor D and factor B. Binding of P to B type HPL was detectable only after incubation of these cells with NHS first reacted with inulin; under these conditions binding of P to Raji cells was also greatly enhanced. With both Raji cells and HPL, factor B was detectable on cell surfaces only after incubation of these cells with NHS first reacted with activators of the P system. Binding of factor B to cells required the presence of C3b and binding or stabilization of cell bound factor B necessitated the presence of activated P. P and factor B were detectable only on cultured cells having C3-C3b receptors. However, incubation of NHS with all lymphoblastoid cell lines studied resulted in activation of P and cleavage of factor B. Binding of P and factor B to cells may follow one of three sequences; (a) activated P in fluid phase combines with C3, factor D, and factor B, and the whole complex fixes to cellular C3-C3b receptors via its C3 moiety; (b) C3b generated in fluid phase combines with P, C3, factor D, and factor B and binds to C3-C3b receptors; or (c) C3 or C3b first binds onto the C3-C3b receptors and thereafter interacts with P, factor D, And factor B. Binding of components of the P system to cells or other particles may relate to such biological phenomena as lysis, phagocytosis, proliferation, attraction of other cell types, and alteration of responsiveness to external stimuli."}
{"id": "PMID:1082489", "title": "Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells.", "content": "Genes mapping in the I region of the H-2 complex control a system of lymphocyte alloantigens (Ia) which are expressed on a subpopulation of T cells and on most B cells. Specific anti-Ia serum in the presence of rabbit complement removed the splenic T-cell subpopulation responsive to Con-A, but did not affect the response to phytohemagglutinin (PHA) or Leucoagglutinin. Antibodies specific for Ia, H-2K, or H-2D membrane antigens were used without complement to pretreat spleen cells. These antibody pretreated cells responded normally to Con-A and PHA.", "contents": "Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells. Genes mapping in the I region of the H-2 complex control a system of lymphocyte alloantigens (Ia) which are expressed on a subpopulation of T cells and on most B cells. Specific anti-Ia serum in the presence of rabbit complement removed the splenic T-cell subpopulation responsive to Con-A, but did not affect the response to phytohemagglutinin (PHA) or Leucoagglutinin. Antibodies specific for Ia, H-2K, or H-2D membrane antigens were used without complement to pretreat spleen cells. These antibody pretreated cells responded normally to Con-A and PHA."}
{"id": "PMID:1082490", "title": "H-2 compatibility requirement for virus-specific T-cell-mediated cytolysis. The H-2K structure involved is coded by a single cistron defined by H-2Kb mutant mice.", "content": "Lymphocytic choriomeningitis or vaccinia virus-immune spleen cells of H-2 mutant mice carrying a point mutation in the K region (B6 H-2ba, B6 H-2bf) cannot lyse infected wild-type H-2Kb targets and vice versa. Yet, cytotoxic T cells specific for infected H-2Kba or H-2Kbf targets are generated during virus infections as shown by cold target competition experiments. The critical structure for the apparent restriction by the K or D regions of the H-2 gene complex of cytolytic interactions between T cells and virus-infected target cells are therefore each coded, at least as shown for the K region, by a single cistron. This finding is most readily accommodated within the altered self concept (postulating that T cells are specific for virus-modified self structures) but cannot exclude the possibility of a physiological interaction mechanism being responsible for the apparent H-2 restriction of virus-specific cytotoxic T cells.", "contents": "H-2 compatibility requirement for virus-specific T-cell-mediated cytolysis. The H-2K structure involved is coded by a single cistron defined by H-2Kb mutant mice. Lymphocytic choriomeningitis or vaccinia virus-immune spleen cells of H-2 mutant mice carrying a point mutation in the K region (B6 H-2ba, B6 H-2bf) cannot lyse infected wild-type H-2Kb targets and vice versa. Yet, cytotoxic T cells specific for infected H-2Kba or H-2Kbf targets are generated during virus infections as shown by cold target competition experiments. The critical structure for the apparent restriction by the K or D regions of the H-2 gene complex of cytolytic interactions between T cells and virus-infected target cells are therefore each coded, at least as shown for the K region, by a single cistron. This finding is most readily accommodated within the altered self concept (postulating that T cells are specific for virus-modified self structures) but cannot exclude the possibility of a physiological interaction mechanism being responsible for the apparent H-2 restriction of virus-specific cytotoxic T cells."}
{"id": "PMID:1082491", "title": "T-lymphocyte-enriched murine peritoneal exudate cells. II. Genetic control of antigen-induced T-lymphocyte proliferation.", "content": "The recent introduction of a reliable, T-lymphocyte proliferation assay, which utilizes thioglycollate-induced, nylon wool column-passed, peritoneal exudate lymphocytes from immune mice (PETLES), allowed us to investigate the genetic control of murine immune responses at the T-lymphocyte level. Examination of the blast cells generated in this population 5 days after stimulation with antigen, revealed that 85% of the cells bore the Thy 1 antigen on their surface, whereas only 5% bore immunoglobulin. Thus, the assay can be considered to measure almost exclusively T-lymphocyte function. This assay was used to examine the T-lymphocyte proliferative responses to seven different antigens: poly(Glu60Ala30Tyr10), poly(Glu58Lys38Tyr4), poly-(Tyr,Glu)-poly-D,L-Ala--poly-Lys, poly-(Phe,Glu)-poly-D,L-Ala--poly-Lys, staphylococcal nuclease, lactate dehydrogenase H4, and the BALB/c IgA myeloma protein, TEPC-15. PETLES from a large number of different inbred mouse strains, including H-2 congenic resistant lines and H-2 recombinants, were studied. The strains could be classified as high responders, low responders, or nonresponders to a particular antigen as judged by the magnitude of the T-lymphocyte proliferative response. In every case but one this classification corresponded to the responder status given the strain based on its ability to mount an in vivo antibody response to the same antigen. For two of the antigens, poly-(Tyr,Glu)-poly-D,L-Ala--poly-Lys and TEPC-15, the immune response genes controlling the T-lymphocyte proliferative response were mapped to the K region or I-A subregion of the major histocompatibility complex, as had previously been shown for the control of the antibody responses to these antigens. This tight linkage of the two phenotypic responses very strongly suggests that the same immune response gene controls the expression of both the proliferative and antibody responses. Since there is essentially no contribution from B lymphocytes in the T-lymphocyte proliferation assay, it seems reasonable to conclude that none of the seven immune response genes studied are expressed solely in B lymphocytes.", "contents": "T-lymphocyte-enriched murine peritoneal exudate cells. II. Genetic control of antigen-induced T-lymphocyte proliferation. The recent introduction of a reliable, T-lymphocyte proliferation assay, which utilizes thioglycollate-induced, nylon wool column-passed, peritoneal exudate lymphocytes from immune mice (PETLES), allowed us to investigate the genetic control of murine immune responses at the T-lymphocyte level. Examination of the blast cells generated in this population 5 days after stimulation with antigen, revealed that 85% of the cells bore the Thy 1 antigen on their surface, whereas only 5% bore immunoglobulin. Thus, the assay can be considered to measure almost exclusively T-lymphocyte function. This assay was used to examine the T-lymphocyte proliferative responses to seven different antigens: poly(Glu60Ala30Tyr10), poly(Glu58Lys38Tyr4), poly-(Tyr,Glu)-poly-D,L-Ala--poly-Lys, poly-(Phe,Glu)-poly-D,L-Ala--poly-Lys, staphylococcal nuclease, lactate dehydrogenase H4, and the BALB/c IgA myeloma protein, TEPC-15. PETLES from a large number of different inbred mouse strains, including H-2 congenic resistant lines and H-2 recombinants, were studied. The strains could be classified as high responders, low responders, or nonresponders to a particular antigen as judged by the magnitude of the T-lymphocyte proliferative response. In every case but one this classification corresponded to the responder status given the strain based on its ability to mount an in vivo antibody response to the same antigen. For two of the antigens, poly-(Tyr,Glu)-poly-D,L-Ala--poly-Lys and TEPC-15, the immune response genes controlling the T-lymphocyte proliferative response were mapped to the K region or I-A subregion of the major histocompatibility complex, as had previously been shown for the control of the antibody responses to these antigens. This tight linkage of the two phenotypic responses very strongly suggests that the same immune response gene controls the expression of both the proliferative and antibody responses. Since there is essentially no contribution from B lymphocytes in the T-lymphocyte proliferation assay, it seems reasonable to conclude that none of the seven immune response genes studied are expressed solely in B lymphocytes."}
{"id": "PMID:1082492", "title": "Participation of the H-2 antigens of tumor cells in their lysis by syngeneic T cells.", "content": "Cytotoxic T lymphocytes were generated in vitro against H-2 compatible or syngeneic tumor cells. In vitro cytotoxic activity was inhibited by specific anti-H2 sera, suggesting that H-2 antigens are involved in cell lysis. Two observations directly demonstrated the participation of the H-2 antigens on the tumor cells in their lysis by H-2-compatible T cells. First, coating of the H-2 antigens on the target tumor cell reduced the number of cells lysed on subsequent exposure to cytotoxic T cells. Second, when cytotoxic T cells were activated against an H-2 compatible tumor and assayed against an H-2-incompatible tumor, anti-H-2 serum that could bind to the target cell, but not to the cytotoxic lymphocyte, inhibited lysis. H-2 antigens were also shown to be present on the cytotoxic lymphocytes. Specific antisera reacting with these H-2 antigens, but not those of the target cell, failed to inhibit lysis when small numbers of effector cells were assayed against H-2-incompatible target cells or when effector cells of F1-hybrid origin and bearing two H-2 haplotypes were assayed against a tumor cell of one of the parental strains. These findings suggest that it is the H-2 antigens on the tumor cell and not those on the cytotoxic lymphocytes that are important in cell-mediated lysis of H-2-compatible tumor cells.", "contents": "Participation of the H-2 antigens of tumor cells in their lysis by syngeneic T cells. Cytotoxic T lymphocytes were generated in vitro against H-2 compatible or syngeneic tumor cells. In vitro cytotoxic activity was inhibited by specific anti-H2 sera, suggesting that H-2 antigens are involved in cell lysis. Two observations directly demonstrated the participation of the H-2 antigens on the tumor cells in their lysis by H-2-compatible T cells. First, coating of the H-2 antigens on the target tumor cell reduced the number of cells lysed on subsequent exposure to cytotoxic T cells. Second, when cytotoxic T cells were activated against an H-2 compatible tumor and assayed against an H-2-incompatible tumor, anti-H-2 serum that could bind to the target cell, but not to the cytotoxic lymphocyte, inhibited lysis. H-2 antigens were also shown to be present on the cytotoxic lymphocytes. Specific antisera reacting with these H-2 antigens, but not those of the target cell, failed to inhibit lysis when small numbers of effector cells were assayed against H-2-incompatible target cells or when effector cells of F1-hybrid origin and bearing two H-2 haplotypes were assayed against a tumor cell of one of the parental strains. These findings suggest that it is the H-2 antigens on the tumor cell and not those on the cytotoxic lymphocytes that are important in cell-mediated lysis of H-2-compatible tumor cells."}
{"id": "PMID:1082493", "title": "Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor.", "content": "The activities of a colony-stimulating factor (CSF), which stimulates granulocyte-macrophage colony formation by mouse hemopoietic cells, and macrophage growth factor (MGF), which stimulates proliferation of activated peritoneal macrophages, have been demonstrated by various criteria to reside in the same molecular species. These criteria include occurrence in various sources and copurification of the activities in mouse L-cell-conditioned medium as well as the biological, physicochemical, and antigenic properties of the activities of L-cell-conditioned medium. CSF and MGF activities of L-cell-conditioned medium are ascribable to a glycoprotein of mol wt approximately 60,000 which migrates electrophoretically with alpha-globulin. Human urinary CSF, which also possesses MGF activity, has similar properties and can be neutralized by antiserum to highly purified L-cell medium CSF. A procedure is described for the partial purification of material from L-cell medium that has activity at 1 ng/ml in both MGF and CSF assays.", "contents": "Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor. The activities of a colony-stimulating factor (CSF), which stimulates granulocyte-macrophage colony formation by mouse hemopoietic cells, and macrophage growth factor (MGF), which stimulates proliferation of activated peritoneal macrophages, have been demonstrated by various criteria to reside in the same molecular species. These criteria include occurrence in various sources and copurification of the activities in mouse L-cell-conditioned medium as well as the biological, physicochemical, and antigenic properties of the activities of L-cell-conditioned medium. CSF and MGF activities of L-cell-conditioned medium are ascribable to a glycoprotein of mol wt approximately 60,000 which migrates electrophoretically with alpha-globulin. Human urinary CSF, which also possesses MGF activity, has similar properties and can be neutralized by antiserum to highly purified L-cell medium CSF. A procedure is described for the partial purification of material from L-cell medium that has activity at 1 ng/ml in both MGF and CSF assays."}
{"id": "PMID:1082494", "title": "The capacity of mitogen-responsive T cells to \"home\" to their tissue of origin, analyzed by means of chromosome markers.", "content": "Lance and Taub (1) showed that when radioactively labeled lymphocytes were injected into a syngeneic mouse and the lymph node cells of this animal transferred to a second syngeneic recipient, the proportion of radioactivity found in the lymph node relative to the amount present in the spleen of the secondary recipient had increased markedly. The interpretation of this result was that some lymphocytes have the capacity to \"home\" to their organ of origin. The purpose of the experiments described here was to test the \"homing\" copacity of T cells by a method that did not involve radioactive labeling. It has been shown elsewhere that some or all mouse T cells are stimulated to divide in culture by the mitogens phytohemagglutinin (PHA) and concanavalin A (Con A) (2). We therefore elected to inject karyotypically distinct lymphocytes into syngeneic recipients and to follow their subsequent distribution by culture of lymph node and spleen cells of the recipient with PHA or Con A. In this manner the homing capacities of spleen and lymph node T cells could be determined, and furthermore, the effects of labeling with chromium-51 ((51)Cr) could be assayed with respect to the persistence of mitogen responsiveness in the injected cells.", "contents": "The capacity of mitogen-responsive T cells to \"home\" to their tissue of origin, analyzed by means of chromosome markers. Lance and Taub (1) showed that when radioactively labeled lymphocytes were injected into a syngeneic mouse and the lymph node cells of this animal transferred to a second syngeneic recipient, the proportion of radioactivity found in the lymph node relative to the amount present in the spleen of the secondary recipient had increased markedly. The interpretation of this result was that some lymphocytes have the capacity to \"home\" to their organ of origin. The purpose of the experiments described here was to test the \"homing\" copacity of T cells by a method that did not involve radioactive labeling. It has been shown elsewhere that some or all mouse T cells are stimulated to divide in culture by the mitogens phytohemagglutinin (PHA) and concanavalin A (Con A) (2). We therefore elected to inject karyotypically distinct lymphocytes into syngeneic recipients and to follow their subsequent distribution by culture of lymph node and spleen cells of the recipient with PHA or Con A. In this manner the homing capacities of spleen and lymph node T cells could be determined, and furthermore, the effects of labeling with chromium-51 ((51)Cr) could be assayed with respect to the persistence of mitogen responsiveness in the injected cells."}
{"id": "PMID:1082495", "title": "Evidence for direct linkage between antigen recognition and lytic expression in effector T cells.", "content": "The relationship between antigen recognition and lytic expression by effector T cells was examined by coculturing two cytolytically active lymphoid cell populations. When antigen recognition between the populations could occur only in one direction, then cytotoxicity was expressed only in that direction and the population whose antigens were recognized lost its lytic activity. In contrast, the cocultured effector cell population fully maintained its lytic potential. This lack of reciprocal inactivation was taken as evidence that T-cell receptor accomodation by surface antigen is linked to the expression of cytolytic activity by effector T lymphocytes.", "contents": "Evidence for direct linkage between antigen recognition and lytic expression in effector T cells. The relationship between antigen recognition and lytic expression by effector T cells was examined by coculturing two cytolytically active lymphoid cell populations. When antigen recognition between the populations could occur only in one direction, then cytotoxicity was expressed only in that direction and the population whose antigens were recognized lost its lytic activity. In contrast, the cocultured effector cell population fully maintained its lytic potential. This lack of reciprocal inactivation was taken as evidence that T-cell receptor accomodation by surface antigen is linked to the expression of cytolytic activity by effector T lymphocytes."}
{"id": "PMID:1082498", "title": "Anatomical observations of the subarachnoid cisterns of the brain during surgery.", "content": "Subarachnoid cisterns and their contents are described briefly in the light of observations made during microsurgical explorations. The concept of intracranial surgery in terms of moving from one cistern to another is presented here with particular emphasis on the cisterns in surgical approaches to intracranial vessels and nerves for the treatment of aneurysms, arteriovenous malformations, and for surgery of basal tumors.", "contents": "Anatomical observations of the subarachnoid cisterns of the brain during surgery. Subarachnoid cisterns and their contents are described briefly in the light of observations made during microsurgical explorations. The concept of intracranial surgery in terms of moving from one cistern to another is presented here with particular emphasis on the cisterns in surgical approaches to intracranial vessels and nerves for the treatment of aneurysms, arteriovenous malformations, and for surgery of basal tumors."}
{"id": "PMID:1082500", "title": "Graded activation of myofibrils and the effect of diameter on tension development during contractures in isolated skeletal muscle fibres.", "content": "If the space constant of the T-system (lambdaT) its not large in comparison with the radius (a) of a muscle fibre, different levels of depolarization should activate different proportions of the cross-section. This possibility was tested in isolated muscle fibres with isotonic and isometric K  contractures. 2. During isonic contractures with more than 40 mM-K, wavy myofibrils appeared in the centre of the fibre. The sarcomere spacings (s) of the wavy myofibrils, measured parallel to the long axis of the myofibrils, were 1-9-1-95 mum. However, the superficial myofibrils could shorten to or below s=1-5 mum without becoming wavy. 3. In the same muscle fibre where myofibrils became wavy during K contractures, no waviness appeared during repetitive electric stimulation in normal Ringer (50 shocks/sec, 12 degrees C), although s decreased below 1-5 mum. Wavy myofibrils were interpreted as not activated. 4. With isometric contractures it was found that the amount of depolarization needed to obtain maximal tension was smaller for fibres of shorter radius. The degree of depolarization for producing maximal tension is related to a by 6 mV/10mum. 5. These results strongly suggest that in K contractures lambdaT is not large in comparison with a.", "contents": "Graded activation of myofibrils and the effect of diameter on tension development during contractures in isolated skeletal muscle fibres. If the space constant of the T-system (lambdaT) its not large in comparison with the radius (a) of a muscle fibre, different levels of depolarization should activate different proportions of the cross-section. This possibility was tested in isolated muscle fibres with isotonic and isometric K  contractures. 2. During isonic contractures with more than 40 mM-K, wavy myofibrils appeared in the centre of the fibre. The sarcomere spacings (s) of the wavy myofibrils, measured parallel to the long axis of the myofibrils, were 1-9-1-95 mum. However, the superficial myofibrils could shorten to or below s=1-5 mum without becoming wavy. 3. In the same muscle fibre where myofibrils became wavy during K contractures, no waviness appeared during repetitive electric stimulation in normal Ringer (50 shocks/sec, 12 degrees C), although s decreased below 1-5 mum. Wavy myofibrils were interpreted as not activated. 4. With isometric contractures it was found that the amount of depolarization needed to obtain maximal tension was smaller for fibres of shorter radius. The degree of depolarization for producing maximal tension is related to a by 6 mV/10mum. 5. These results strongly suggest that in K contractures lambdaT is not large in comparison with a."}
{"id": "PMID:1082501", "title": "Electromechanical studies on the inotropic effects of acetylstrophanthidin in ventricular muscle.", "content": "Three phases in the inotropic response of acetyl strophanthidin (AcS) on the electromechnical activity of the frog ventricular myocardium were identified and studied using a single sucrose voltage-clamp technique and other conventional electrophysiological methods. 2. the positive inotropic response of the drug was accompanied by a shift in tension-voltage relation, so that more tension developed with every depolarization step above the mechanical threshold (-50mV). Only at higher drug concentrations or with long exposure times did the mechanical threshold shift to more negative membrane potentials (-60 to-70 mV). 3. In tetrodotoxin-treated muscles AcS produced marked potentiation of twitch tension and an appropriate shift in the tension-voltage relation. 4. the positive inotropic response of the drug was not related to the magnitude of the direction of the fast or slow Na current. 5. in tetrodotoxin-treated ventricular strips the direction or the magnitude of the secondary inward current (ICa or INa) were not related to the inotropic effect of AcS. 6. AcS shortens the action potential markedly during the later stages of its positive inotropic response. When Ca2+ is omitted from the bathing solution AcS not only fails to shorten the action potential, but often prolongs it. 7. The shortening of the action potential in the presence of AcS is accompanied by an increase in the \"instantaneous\" membrane conductance both at rest and during the time course of the plateau. 8. The decline in the positive inotropic response of the drug was accompanied by the shortening of the action potential. Electrical or chemical prolongation of the action potential restored the full positive inotropic response if the membrane had not depolarized. 9. Membrane depolarization and the development of diastolic tension always occurred at later stages of drug action. Elevation of [Mg+2]degrees to 5 or 10 mM prevented or suppressed the membrane depolarization and the diastolic tension. 10. KCl-induced contractures were potentiated throughout the duration of drug exposure. The tonic component of the contracture tension was markedly elevated especially at later stages of drug action. 11. The experimental evidence suggests that no unitary mechanism could account for multiple actions of acetyl strophanthidin. However, the contributions of the Na pump, the Ca+2 sequestering system, and the K-efflux system to the various stages of drug action are discussed.", "contents": "Electromechanical studies on the inotropic effects of acetylstrophanthidin in ventricular muscle. Three phases in the inotropic response of acetyl strophanthidin (AcS) on the electromechnical activity of the frog ventricular myocardium were identified and studied using a single sucrose voltage-clamp technique and other conventional electrophysiological methods. 2. the positive inotropic response of the drug was accompanied by a shift in tension-voltage relation, so that more tension developed with every depolarization step above the mechanical threshold (-50mV). Only at higher drug concentrations or with long exposure times did the mechanical threshold shift to more negative membrane potentials (-60 to-70 mV). 3. In tetrodotoxin-treated muscles AcS produced marked potentiation of twitch tension and an appropriate shift in the tension-voltage relation. 4. the positive inotropic response of the drug was not related to the magnitude of the direction of the fast or slow Na current. 5. in tetrodotoxin-treated ventricular strips the direction or the magnitude of the secondary inward current (ICa or INa) were not related to the inotropic effect of AcS. 6. AcS shortens the action potential markedly during the later stages of its positive inotropic response. When Ca2+ is omitted from the bathing solution AcS not only fails to shorten the action potential, but often prolongs it. 7. The shortening of the action potential in the presence of AcS is accompanied by an increase in the \"instantaneous\" membrane conductance both at rest and during the time course of the plateau. 8. The decline in the positive inotropic response of the drug was accompanied by the shortening of the action potential. Electrical or chemical prolongation of the action potential restored the full positive inotropic response if the membrane had not depolarized. 9. Membrane depolarization and the development of diastolic tension always occurred at later stages of drug action. Elevation of [Mg+2]degrees to 5 or 10 mM prevented or suppressed the membrane depolarization and the diastolic tension. 10. KCl-induced contractures were potentiated throughout the duration of drug exposure. The tonic component of the contracture tension was markedly elevated especially at later stages of drug action. 11. The experimental evidence suggests that no unitary mechanism could account for multiple actions of acetyl strophanthidin. However, the contributions of the Na pump, the Ca+2 sequestering system, and the K-efflux system to the various stages of drug action are discussed."}
{"id": "PMID:1082505", "title": "Electrical responses to frog taste cells to chemical stimuli.", "content": "1. Cells inside a fungiform papilla of the frog tongue were impaled with a glass capillary micro-electrode filled with 3 M-KCl. Cells considered to be taste cells showed a resting potential of about -35 mV and an input resistance of 17 Momega on the average. 2. Application of chemical stimuli such as salts, acids and quinine produced a sustained depolarization in a taste cell, the magnitude of depolarization being dependent on the stimulus concentration. Water and weak NaCl solution yielded a hyperpolarization. The thresholds for depolarization as well as the concentration-response relationships for various chemical stimuli in taste cells are in approximate agreement with those determined from the glossopharyngeal nerve responses. 3. The magnitude of depolarization produced by 0-1 M-NaCl and 0-03 M-CaCl2 was dependent on the membrane potential level and reduced linearly with a rise in the latter. However, depolarizations generated by 0-001 M-HDl and 0-02 M quinine changed little in magnitude by a membrane potential change over a wide range. 4. During depolarizations induced by NaCl and KCl a marked reduction in the input resistance of a cell was observed, the amount of the reduction depending on the stimulus concentration. The reduction was also produced by CaCl2 and HCl, but it is small compared with those by NaCl and KCl. Quinine produced an increase in the resistance associated with a depolarization. Water and weak NaCl solution produced an increase in the resistance associated with hyperpolarization. 5. The receptive mechanisms for various kinds of chemical stimuli are discussed in relation to changes in the membrane potential and the membrane conductance of taste cells.", "contents": "Electrical responses to frog taste cells to chemical stimuli. 1. Cells inside a fungiform papilla of the frog tongue were impaled with a glass capillary micro-electrode filled with 3 M-KCl. Cells considered to be taste cells showed a resting potential of about -35 mV and an input resistance of 17 Momega on the average. 2. Application of chemical stimuli such as salts, acids and quinine produced a sustained depolarization in a taste cell, the magnitude of depolarization being dependent on the stimulus concentration. Water and weak NaCl solution yielded a hyperpolarization. The thresholds for depolarization as well as the concentration-response relationships for various chemical stimuli in taste cells are in approximate agreement with those determined from the glossopharyngeal nerve responses. 3. The magnitude of depolarization produced by 0-1 M-NaCl and 0-03 M-CaCl2 was dependent on the membrane potential level and reduced linearly with a rise in the latter. However, depolarizations generated by 0-001 M-HDl and 0-02 M quinine changed little in magnitude by a membrane potential change over a wide range. 4. During depolarizations induced by NaCl and KCl a marked reduction in the input resistance of a cell was observed, the amount of the reduction depending on the stimulus concentration. The reduction was also produced by CaCl2 and HCl, but it is small compared with those by NaCl and KCl. Quinine produced an increase in the resistance associated with a depolarization. Water and weak NaCl solution produced an increase in the resistance associated with hyperpolarization. 5. The receptive mechanisms for various kinds of chemical stimuli are discussed in relation to changes in the membrane potential and the membrane conductance of taste cells."}
{"id": "PMID:1082506", "title": "A non-linear voltage dependent charge movement in frog skeletal muscle.", "content": "1. Voltage-clamp experiments were carried out using the three microelectrode technique. Using this method membrane current density at V1 is proportional to deltaV( = V2 - V1) where V1 and V2 are voltages at distances 1 and 21 from the end of a fibre. Voltage dependent sodium currents were blocked by tetrodotoxin, potassium by tetraethylammonium ions and rubidium. Contraction was blocked by adding sucrose, 467 mM. 2. The current deltaV (control) associated with a positive voltage step from a hyperpolarized conditioning voltage to the holding potential, -80 mV, showed two components, a capacitative transient which decayed rapidly and a maintained steady level...", "contents": "A non-linear voltage dependent charge movement in frog skeletal muscle. 1. Voltage-clamp experiments were carried out using the three microelectrode technique. Using this method membrane current density at V1 is proportional to deltaV( = V2 - V1) where V1 and V2 are voltages at distances 1 and 21 from the end of a fibre. Voltage dependent sodium currents were blocked by tetrodotoxin, potassium by tetraethylammonium ions and rubidium. Contraction was blocked by adding sucrose, 467 mM. 2. The current deltaV (control) associated with a positive voltage step from a hyperpolarized conditioning voltage to the holding potential, -80 mV, showed two components, a capacitative transient which decayed rapidly and a maintained steady level..."}
{"id": "PMID:1082507", "title": "Effects of glycerol treatment and maintained depolarization on charge movement in skeletal muscle.", "content": "1. Voltage-clamp experiments were carried out using the techniques described in the preceding paper. 2. In one series of experiments an attempt was made to disrupt the T-system with glycerol treatment. Muscles were soaked in Ringer + 400 mM glycerol for 1 hr at room temperature, transferred to Ringer + 5 mM calcium + 5 mM magnesium for 20-30 min, and then cooled to around 2 degrees C and placed in an isosmotic test solution containing tetrodotoxin for electrical measurements. 3. The density of charge seen in isosmotic tetraethylammonium (TEA) solution with strong depolarization, normalized according to fibre capacitance, was decreased by glycerol treatment to about one third the amount seen in untreated hypertonic fibres. 4. An analysis of fibre capacitance revealed that only 0-4 of the tubular capacitance was removed by this particular glycerol procedure. If the density of charge with respect to capacitance is corrected for this decrease in capacitance, the results indicate that glycerol treatment removed or immobilized 0-77 of the charge initially present. Thus the effect of glycerol treatment to reduce charge does not depend entirely on disrupting the electrical continuity of the T-system. 5. The effects of maintained depolarization were studied using a TEA Ringer made hypertonic with sucrose. When the voltage was changed from -80 to -21 mV the measurable charge movement declined exponentially to zero with a time constant of 13-24 sec. On repolarization the process recovered exponentially to the initial level with a time constant of 21-53 sec. 6. Experiments were also carried out using a sodium Ringer made hypertonic with sucrose. For small depolarizations only charge movement currents were seen, whereas for large depolarizations large delayed ionic currents, presumably carried by potassium, were observed. With moderate depolarizations in the range V = -40 to -30 mV, both components were of comparable magnitude. 7. A plot of the fractional charge movement (Q/Qmax) vs. V fitted at moderate depolarizations is similar to that of n infinity vs. V fitted at larger depolarizations. Values of n infinity were obtained by fitting the delayed ionic current to n4(V - VK). For voltages between -40 and -30 mV the time constant for charge tauQ was always less than taun; values of taun/tauQ varied from 1-6 to 4-3. 8. Glycerol treatment had little if any effect on the kinetics of delayed rectifier currents. Values of gK measured in isosmotic solution following glycerol were about one third the values obtained in untreated fibres in a hypertonic solution (osmolality three times normal). The threefold difference in gK is probably due to a similar difference in internal potassium concentration. 9. These results and others are difficult to reconcile with the idea that the charge movement process acts as a gate for potassium channels. It seems more likely that charge movement is a step in the activation of contraction.", "contents": "Effects of glycerol treatment and maintained depolarization on charge movement in skeletal muscle. 1. Voltage-clamp experiments were carried out using the techniques described in the preceding paper. 2. In one series of experiments an attempt was made to disrupt the T-system with glycerol treatment. Muscles were soaked in Ringer + 400 mM glycerol for 1 hr at room temperature, transferred to Ringer + 5 mM calcium + 5 mM magnesium for 20-30 min, and then cooled to around 2 degrees C and placed in an isosmotic test solution containing tetrodotoxin for electrical measurements. 3. The density of charge seen in isosmotic tetraethylammonium (TEA) solution with strong depolarization, normalized according to fibre capacitance, was decreased by glycerol treatment to about one third the amount seen in untreated hypertonic fibres. 4. An analysis of fibre capacitance revealed that only 0-4 of the tubular capacitance was removed by this particular glycerol procedure. If the density of charge with respect to capacitance is corrected for this decrease in capacitance, the results indicate that glycerol treatment removed or immobilized 0-77 of the charge initially present. Thus the effect of glycerol treatment to reduce charge does not depend entirely on disrupting the electrical continuity of the T-system. 5. The effects of maintained depolarization were studied using a TEA Ringer made hypertonic with sucrose. When the voltage was changed from -80 to -21 mV the measurable charge movement declined exponentially to zero with a time constant of 13-24 sec. On repolarization the process recovered exponentially to the initial level with a time constant of 21-53 sec. 6. Experiments were also carried out using a sodium Ringer made hypertonic with sucrose. For small depolarizations only charge movement currents were seen, whereas for large depolarizations large delayed ionic currents, presumably carried by potassium, were observed. With moderate depolarizations in the range V = -40 to -30 mV, both components were of comparable magnitude. 7. A plot of the fractional charge movement (Q/Qmax) vs. V fitted at moderate depolarizations is similar to that of n infinity vs. V fitted at larger depolarizations. Values of n infinity were obtained by fitting the delayed ionic current to n4(V - VK). For voltages between -40 and -30 mV the time constant for charge tauQ was always less than taun; values of taun/tauQ varied from 1-6 to 4-3. 8. Glycerol treatment had little if any effect on the kinetics of delayed rectifier currents. Values of gK measured in isosmotic solution following glycerol were about one third the values obtained in untreated fibres in a hypertonic solution (osmolality three times normal). The threefold difference in gK is probably due to a similar difference in internal potassium concentration. 9. These results and others are difficult to reconcile with the idea that the charge movement process acts as a gate for potassium channels. It seems more likely that charge movement is a step in the activation of contraction."}
{"id": "PMID:1082508", "title": "The voltage dependence of membrane capacity.", "content": "1. Membrane capacity of sartorius muscle fibres has been measured at membrane potentials between -200 and +50 mV. Within this potential range the capacity is not independent of potential. Dielectric saturation is present at large negative and at positive internal potentials, indicating the presence in the membrane of permanent dipoles or movable charges. 2. In normally polarized fibres there is a sharp peak in the capacity-potential relation of about -50 mV; the capacity at this peak is 50% larger than the capacity at -90 mV. 3. In depolarized fibres this sharp peak of capacity is not present. Over the range -200 to +50 mV the capacity variation is about 10% with a broad maximum at about -80 mV. 4. The dielectric behaviour of muscle membrane is most simply explained by postulating two species of permanent dipoles or mobile charges: Charge 1 present in normally polarized fibres, but neutralized or immobilized in depolarized fibres; Charge 2 present in both polarized and depolarized fibres. The distribution of Charge 1 is more steeply voltage-dependent than is the distribution of Charge 2. 5. Movement of Charge 1 from one fully saturated configuration to the other involves a charge transfer across the membrane of between 20 and 30 nC/muF. Movement of Charge 2 in depolarized fibres requires a similar transfer of charge.", "contents": "The voltage dependence of membrane capacity. 1. Membrane capacity of sartorius muscle fibres has been measured at membrane potentials between -200 and +50 mV. Within this potential range the capacity is not independent of potential. Dielectric saturation is present at large negative and at positive internal potentials, indicating the presence in the membrane of permanent dipoles or movable charges. 2. In normally polarized fibres there is a sharp peak in the capacity-potential relation of about -50 mV; the capacity at this peak is 50% larger than the capacity at -90 mV. 3. In depolarized fibres this sharp peak of capacity is not present. Over the range -200 to +50 mV the capacity variation is about 10% with a broad maximum at about -80 mV. 4. The dielectric behaviour of muscle membrane is most simply explained by postulating two species of permanent dipoles or mobile charges: Charge 1 present in normally polarized fibres, but neutralized or immobilized in depolarized fibres; Charge 2 present in both polarized and depolarized fibres. The distribution of Charge 1 is more steeply voltage-dependent than is the distribution of Charge 2. 5. Movement of Charge 1 from one fully saturated configuration to the other involves a charge transfer across the membrane of between 20 and 30 nC/muF. Movement of Charge 2 in depolarized fibres requires a similar transfer of charge."}
{"id": "PMID:1082509", "title": "Charge movement in the membrane of striated muscle.", "content": "1. Non-linear polarization currents apparently due to permanent dipoles or mobile charges in the membrane can be measured by appropriate comparison of the transient currents required to produce small and large steps of membrane potential. Integration of these transient polarization currents estimates the charge transfer associated with the movement of membrane dipoles or charges. 2. Depolarization from -100 to 0 mV requires a charge transfer of 35 nC/muF in addition to the charge transfer predicted by linear extrapolation of the charge required for a small depolarization from -100 mV. Depolarizations of varying size give a charge-voltage relation which is sigmoid saturating beyond o mV and with a midpoint at about -50 mV. The ratnged depolarization reduces or removes charge movement detected by comparing currents for small and large voltage steps from -100 mV (Charge 1). However in depolarized fibres comparison of currents from a small potential step at +40 mV and a large hyperpolarizing potential step from -20 mV reveals large movements of a second charge (Charge 2). Movement of Charge 2 is less steeply dependent on voltage than movement of Charge 2 both in magnitude and in rate. 4. In size and voltage dependence these two kinds of charge movement correspond to measured voltage dependence of capacity in normally polarized and depolarized fibres (Adrian & Almers, 1976).", "contents": "Charge movement in the membrane of striated muscle. 1. Non-linear polarization currents apparently due to permanent dipoles or mobile charges in the membrane can be measured by appropriate comparison of the transient currents required to produce small and large steps of membrane potential. Integration of these transient polarization currents estimates the charge transfer associated with the movement of membrane dipoles or charges. 2. Depolarization from -100 to 0 mV requires a charge transfer of 35 nC/muF in addition to the charge transfer predicted by linear extrapolation of the charge required for a small depolarization from -100 mV. Depolarizations of varying size give a charge-voltage relation which is sigmoid saturating beyond o mV and with a midpoint at about -50 mV. The ratnged depolarization reduces or removes charge movement detected by comparing currents for small and large voltage steps from -100 mV (Charge 1). However in depolarized fibres comparison of currents from a small potential step at +40 mV and a large hyperpolarizing potential step from -20 mV reveals large movements of a second charge (Charge 2). Movement of Charge 2 is less steeply dependent on voltage than movement of Charge 2 both in magnitude and in rate. 4. In size and voltage dependence these two kinds of charge movement correspond to measured voltage dependence of capacity in normally polarized and depolarized fibres (Adrian & Almers, 1976)."}
{"id": "PMID:1082510", "title": "Charge movement and mechanical repriming in skeletal muscle.", "content": "1. Muscles were placed in a solution which depolarized the membrane to -30 to -20 mV so that mechanical activation was made refractory. Mechanical repriming and the recovery of voltage dependent charge movement were studied using a voltage clamp technique. 2. Mechanical repriming was investigated by determining the duration of a hyperpolarizing pulse required to elicit a just-visible contraction for various post-pulse potentials. As the post-pulse potential was made more positive shorter repriming times were required to produce a threshold contraction. The relationship approached a minimum repriming time for very positive post-pulse potentials. 3. These results suggest that hyperpolarization gradually removes some component of the activation mechanism from a refractory state and that the effectiveness of the amount which has recovered depends on the post-pulse potential. A quantitative explanation is given using a simple model in which the essential component is assumed to be the charge movement process. 4. The rate of repriming contraction is voltage dependent; at -160 mV the rate is about twice that at -120 mV. Between 4 and 10 degrees C the rate has a Q10 of about 9. 5. Recovery of charge movement was studied using a repriming duration less than that required to produce a threshold contraction. The observed charge movement increased linearly with repriming time, consistent with the approximately linear initial segment of a slow exponential recovery process. Extrapolation of the recovery curve indicated that 2-5 n/CmuF of charge is reprimed in the time necessary to reprime a threshold contraction. 6. The charge which recovers during a subthreshold repriming pulse is distributed according to membrane potential in the same way as a fully reprimed charge. 7. These results are consistent with the hypothesis that voltage dependent charge movement is an intermediate step in excitation-contraction coupling. 8. The characteristics of a second type of charge movement are also described.", "contents": "Charge movement and mechanical repriming in skeletal muscle. 1. Muscles were placed in a solution which depolarized the membrane to -30 to -20 mV so that mechanical activation was made refractory. Mechanical repriming and the recovery of voltage dependent charge movement were studied using a voltage clamp technique. 2. Mechanical repriming was investigated by determining the duration of a hyperpolarizing pulse required to elicit a just-visible contraction for various post-pulse potentials. As the post-pulse potential was made more positive shorter repriming times were required to produce a threshold contraction. The relationship approached a minimum repriming time for very positive post-pulse potentials. 3. These results suggest that hyperpolarization gradually removes some component of the activation mechanism from a refractory state and that the effectiveness of the amount which has recovered depends on the post-pulse potential. A quantitative explanation is given using a simple model in which the essential component is assumed to be the charge movement process. 4. The rate of repriming contraction is voltage dependent; at -160 mV the rate is about twice that at -120 mV. Between 4 and 10 degrees C the rate has a Q10 of about 9. 5. Recovery of charge movement was studied using a repriming duration less than that required to produce a threshold contraction. The observed charge movement increased linearly with repriming time, consistent with the approximately linear initial segment of a slow exponential recovery process. Extrapolation of the recovery curve indicated that 2-5 n/CmuF of charge is reprimed in the time necessary to reprime a threshold contraction. 6. The charge which recovers during a subthreshold repriming pulse is distributed according to membrane potential in the same way as a fully reprimed charge. 7. These results are consistent with the hypothesis that voltage dependent charge movement is an intermediate step in excitation-contraction coupling. 8. The characteristics of a second type of charge movement are also described."}
{"id": "PMID:1082511", "title": "Chemistry and biological activities of N,N-dimethylaminoethyl acrylate, a choline acetyltransferase inhibitor.", "content": "N,N-Dimethylaminoethyl acrylate (acryl-DMA) was synthesized as a tertiary nitrogen choline acetyltransferase (ChAc) inhibitor which would be able to penetrate biological membranes to inhibit ChAc in the nerve terminal. The synthesis from dimethylaminoethanol and acrylyl chloride was described and the hydration with times in an aqueous medium measured by NMR spectroscopy was presented. The autohydrolysis in water was found to be 1.75 x 10(-8) mol/min at pH 7.4 and 5.0 mM concentration. The enzymatic hydrolysis was unaffected by cholinesterases. Acryl-DMA was capable of inhibiting ChAc extracted from rat brain with I50 of 5.02 x 10(-4) M. The inhibition was reversible and displayed uncompetitive kinetics with respect to both substrates, choline and acetyl-CoA. Neither the hydrolysis nor the hydration products of acryl-DMA could inhibit ChAc. Although acryl-DMA was hydrated rapidly and completely within 1 hr at high pH (9.0), the time course of inhibition ability of acryl-DMA in aqueous medium at physiological pH was found to decrease rather slowly and by 36% in 1 hr, indicating that acryl-DMA can survive from hydration at physiological pH. Acryl-DMA was also tested for its ability to block electrically induced muscle contractions in both isolated skeletal and smooth nerve-muscle preparations. The ED50's obtained were less than 5 x 10(-4) M in both cases.", "contents": "Chemistry and biological activities of N,N-dimethylaminoethyl acrylate, a choline acetyltransferase inhibitor. N,N-Dimethylaminoethyl acrylate (acryl-DMA) was synthesized as a tertiary nitrogen choline acetyltransferase (ChAc) inhibitor which would be able to penetrate biological membranes to inhibit ChAc in the nerve terminal. The synthesis from dimethylaminoethanol and acrylyl chloride was described and the hydration with times in an aqueous medium measured by NMR spectroscopy was presented. The autohydrolysis in water was found to be 1.75 x 10(-8) mol/min at pH 7.4 and 5.0 mM concentration. The enzymatic hydrolysis was unaffected by cholinesterases. Acryl-DMA was capable of inhibiting ChAc extracted from rat brain with I50 of 5.02 x 10(-4) M. The inhibition was reversible and displayed uncompetitive kinetics with respect to both substrates, choline and acetyl-CoA. Neither the hydrolysis nor the hydration products of acryl-DMA could inhibit ChAc. Although acryl-DMA was hydrated rapidly and completely within 1 hr at high pH (9.0), the time course of inhibition ability of acryl-DMA in aqueous medium at physiological pH was found to decrease rather slowly and by 36% in 1 hr, indicating that acryl-DMA can survive from hydration at physiological pH. Acryl-DMA was also tested for its ability to block electrically induced muscle contractions in both isolated skeletal and smooth nerve-muscle preparations. The ED50's obtained were less than 5 x 10(-4) M in both cases."}
{"id": "PMID:1082513", "title": "Absence of heart-rate effects in isolated frog heart irradiated with pulse modulated microwave energy.", "content": "Isolated frog hearts were irradiated with pulse modulated microwave energy synchronized with the ECG. No statistically significant or otherwise observable differences were found between the heart rate of irradiated groups and the nonirradiated control group. Experiments were performed to explore the possible effects of currents induced between the recording electrodes. Increases in heart rate occurred when applied current pulses between 20 and 30 mA were synchronized with the ECG during an interval from 200 msec to 300 msec after the peak of the P wave.", "contents": "Absence of heart-rate effects in isolated frog heart irradiated with pulse modulated microwave energy. Isolated frog hearts were irradiated with pulse modulated microwave energy synchronized with the ECG. No statistically significant or otherwise observable differences were found between the heart rate of irradiated groups and the nonirradiated control group. Experiments were performed to explore the possible effects of currents induced between the recording electrodes. Increases in heart rate occurred when applied current pulses between 20 and 30 mA were synchronized with the ECG during an interval from 200 msec to 300 msec after the peak of the P wave."}
{"id": "PMID:1082512", "title": "Lithium transport across isolated frog skin epithelium.", "content": "Transepithelial Li+ influx was studied in the isolated epithelium from abdominal skin of Rana catesbeiana. With Na+-Ringer's as inside medium and Li+-Ringer's as outside medium, the Li+ influx across the epithelium was 15.6 muA/cm2. This influx was considerably reduced by removal of either Na+ or K+ from the inside bath or by the addition of ouabain or amiloride. Epithelial K+ or Na+ concentration was respectively lower in epithelia bathed in K+-free Ringer's or Na+-free Ringer's. In conditions of negligible Na+ transport, a 20 mM Li+ gradient (outleads toin) produced across the short-circuited epithelium a Li+ influx of 11.8 muA/cm2 and a mean short-circuit current of 10.2 muA/cm2. The same Li+ gradient in the opposite direction produced a Li+ outflux of only 1.9 muA/cm2. With equal Li+ concentration (10.3 and 20.6 mM) on both sides of the epihelium, plus Na+ in the inside solution only, a stable Li+-dependent short-circuit current was observed. Net Li+ movement (outleads toin) was also indirectly determined in the presence of an opposing Li+ gradient. Although Li+ does not substitute for Na+ as an activator to the (Na+ +K+)-ATPase from frog skin epithelium, Li+ influx appears to be related to Na+-K+ pump activity. It is proposed that the permeability of the \"outer barrier\" to Na+ and Li+ is regulated by the electrical gradient produced by electrogenic Na+-K+ pumps located in the membrane of the deeper epithelial cells.", "contents": "Lithium transport across isolated frog skin epithelium. Transepithelial Li+ influx was studied in the isolated epithelium from abdominal skin of Rana catesbeiana. With Na+-Ringer's as inside medium and Li+-Ringer's as outside medium, the Li+ influx across the epithelium was 15.6 muA/cm2. This influx was considerably reduced by removal of either Na+ or K+ from the inside bath or by the addition of ouabain or amiloride. Epithelial K+ or Na+ concentration was respectively lower in epithelia bathed in K+-free Ringer's or Na+-free Ringer's. In conditions of negligible Na+ transport, a 20 mM Li+ gradient (outleads toin) produced across the short-circuited epithelium a Li+ influx of 11.8 muA/cm2 and a mean short-circuit current of 10.2 muA/cm2. The same Li+ gradient in the opposite direction produced a Li+ outflux of only 1.9 muA/cm2. With equal Li+ concentration (10.3 and 20.6 mM) on both sides of the epihelium, plus Na+ in the inside solution only, a stable Li+-dependent short-circuit current was observed. Net Li+ movement (outleads toin) was also indirectly determined in the presence of an opposing Li+ gradient. Although Li+ does not substitute for Na+ as an activator to the (Na+ +K+)-ATPase from frog skin epithelium, Li+ influx appears to be related to Na+-K+ pump activity. It is proposed that the permeability of the \"outer barrier\" to Na+ and Li+ is regulated by the electrical gradient produced by electrogenic Na+-K+ pumps located in the membrane of the deeper epithelial cells."}
{"id": "PMID:1082525", "title": "[Aphakia and mesopic vision (author's transl)].", "content": "The results of investigations on the twilight vision of aphakic eyes are given. In the daylight seeing range all the subjects had satisfactory visual acuity, whereas in the mesopic range, on corresponding tests, definite difficulties were recognisable for a large proportion of the subjects.", "contents": "[Aphakia and mesopic vision (author's transl)]. The results of investigations on the twilight vision of aphakic eyes are given. In the daylight seeing range all the subjects had satisfactory visual acuity, whereas in the mesopic range, on corresponding tests, definite difficulties were recognisable for a large proportion of the subjects."}
{"id": "PMID:1082527", "title": "[Separation and immunological characterization of lymphocytes isolated from human appendix (author's transl)].", "content": "The isolation of viable lymphocytes from fresh human appendices is described. Under standardized experimental conditions of cell preparation no selective enrichment of either T- or B-lymphocytes was observed. The mean percentage of T-lymphocytes as judged by spontaneous rosette formation found in 34 appendices examined was 50.0%. After overnight incubation surface-Ig was detected by immunoradioautography on 46.8% of the recovered lymphocytes from 10 appendices. For the detection of surface-Ig specifically purified antibodies to heavy chains mu, gamma and alpha were used. Of the 46.8% surface-Ig positive lymphocytes the class distribution was as follows: IgM 17.7%, IgG 24.4% and IgA 4.7%. The isolated lymphocytes showed a variable mitotic response to PHA (stimulation index ranging from 3.3 to 200.9) as well as to allogeneic lymphocytes in a one-way mixed lymphocyte culture. So far, a distinct correlation could not be found between the histological degree of inflammation and the class or mitotic response of appendix lymphocytes.", "contents": "[Separation and immunological characterization of lymphocytes isolated from human appendix (author's transl)]. The isolation of viable lymphocytes from fresh human appendices is described. Under standardized experimental conditions of cell preparation no selective enrichment of either T- or B-lymphocytes was observed. The mean percentage of T-lymphocytes as judged by spontaneous rosette formation found in 34 appendices examined was 50.0%. After overnight incubation surface-Ig was detected by immunoradioautography on 46.8% of the recovered lymphocytes from 10 appendices. For the detection of surface-Ig specifically purified antibodies to heavy chains mu, gamma and alpha were used. Of the 46.8% surface-Ig positive lymphocytes the class distribution was as follows: IgM 17.7%, IgG 24.4% and IgA 4.7%. The isolated lymphocytes showed a variable mitotic response to PHA (stimulation index ranging from 3.3 to 200.9) as well as to allogeneic lymphocytes in a one-way mixed lymphocyte culture. So far, a distinct correlation could not be found between the histological degree of inflammation and the class or mitotic response of appendix lymphocytes."}
{"id": "PMID:1082531", "title": "Unilateral renal disease in the rat. II. Glomerular mesangial uptake of colloidal carbon in unilateral aminonucleoside nephrosis and nephrotoxic serum nephritis.", "content": "Unilateral renal disease was produced in rats by left renal perfusion in situ with the aminonucleoside of puromycin or with nephrotoxic serum. The uptake of colloidal carbon by the glomerular mesangium was increased in kidneys perfused with aminonucleoside or nephrotoxic serum compared to contralateral kidneys. The quantities of carbon within the glomerular mesangium remained increased in aminonucleoside perfused kidneys 14 days later. These studies demonstrate that alterations in glomerular mesangial function in aminonucleoside nephrosis and nephrotoxic serum nephritis are related to renal factors rather than differences in host milieu and may be involved in the pathogenesis of the morphologic lesions seen in chronic proteinuria.", "contents": "Unilateral renal disease in the rat. II. Glomerular mesangial uptake of colloidal carbon in unilateral aminonucleoside nephrosis and nephrotoxic serum nephritis. Unilateral renal disease was produced in rats by left renal perfusion in situ with the aminonucleoside of puromycin or with nephrotoxic serum. The uptake of colloidal carbon by the glomerular mesangium was increased in kidneys perfused with aminonucleoside or nephrotoxic serum compared to contralateral kidneys. The quantities of carbon within the glomerular mesangium remained increased in aminonucleoside perfused kidneys 14 days later. These studies demonstrate that alterations in glomerular mesangial function in aminonucleoside nephrosis and nephrotoxic serum nephritis are related to renal factors rather than differences in host milieu and may be involved in the pathogenesis of the morphologic lesions seen in chronic proteinuria."}
{"id": "PMID:1082532", "title": "Medical-surgical aspects of left main coronary artery disease.", "content": "Disease of the left main coronary artery compromises circulation to the major part of the left ventricle and thus threatens massive myocardial infarction and sudden death. Cardiac catheterization and coronary bypass surgery, in previous reports, have been associated with high mortality and morbidity rates. We report 50 patients with over 50 per cent narrowing of the left main coronary artery. The clinical pattern in these patients was variable and a left main coronary artery lesion could not be predicted before coronary angiography. There was only one death during cardiac catheterization. One patient died while waiting for elective surgery. Coronary bypass surgery was performed in 42 patients; one died during surgery. Forty-one patients are alive at 2 to 39 months follow-up (mean, 19 months). Thirty-six patients are asymptomatic or have minimal symptoms. Compared to the prognosis in patients with left main coronary artery stenosis treated medically, coronary bypass surgery performed on urgent basis offers a much better prognosis. Both coronary angiography and bypass surgery can be performed in these patients with a very low risk.", "contents": "Medical-surgical aspects of left main coronary artery disease. Disease of the left main coronary artery compromises circulation to the major part of the left ventricle and thus threatens massive myocardial infarction and sudden death. Cardiac catheterization and coronary bypass surgery, in previous reports, have been associated with high mortality and morbidity rates. We report 50 patients with over 50 per cent narrowing of the left main coronary artery. The clinical pattern in these patients was variable and a left main coronary artery lesion could not be predicted before coronary angiography. There was only one death during cardiac catheterization. One patient died while waiting for elective surgery. Coronary bypass surgery was performed in 42 patients; one died during surgery. Forty-one patients are alive at 2 to 39 months follow-up (mean, 19 months). Thirty-six patients are asymptomatic or have minimal symptoms. Compared to the prognosis in patients with left main coronary artery stenosis treated medically, coronary bypass surgery performed on urgent basis offers a much better prognosis. Both coronary angiography and bypass surgery can be performed in these patients with a very low risk."}
{"id": "PMID:1082528", "title": "[Effect of centrifugal force on neck nystagmus in pigeons].", "content": "The neck nystagmus and its changes under the influence of an otolith stimulus--centrifugal force were studied in pigeons. The results show that centrifugal forces exert a dynamic effect on the nystagmus.", "contents": "[Effect of centrifugal force on neck nystagmus in pigeons]. The neck nystagmus and its changes under the influence of an otolith stimulus--centrifugal force were studied in pigeons. The results show that centrifugal forces exert a dynamic effect on the nystagmus."}
{"id": "PMID:1082529", "title": "[Dynamics of motor components of optokinetic nystagmus during exposure of the vestibular apparatus to angular accelerations].", "content": "The effect of angular accelerations on the optokinetic nystagmus was studied on 25 test subjects using a special vestibulo-optokinetic trainer rotated according to five programs and electrooculography. Angular accelerations were found to induce indirectly changes in the amplitude and time of the optokinetic nystagmus via the vestibular apparatus. Optimization of parameters of the optokinetic nystagmus was reflected in the criterion of positive difference + delta between angular velocities of the slow component of the nystagmus and optokinetic stimuli. It is postulated that the criterion + delta is a necessary factor in the organization of optimal time relations between optokinetic stimuli and central processes that are responsible for the final result--sensory image of movement.", "contents": "[Dynamics of motor components of optokinetic nystagmus during exposure of the vestibular apparatus to angular accelerations]. The effect of angular accelerations on the optokinetic nystagmus was studied on 25 test subjects using a special vestibulo-optokinetic trainer rotated according to five programs and electrooculography. Angular accelerations were found to induce indirectly changes in the amplitude and time of the optokinetic nystagmus via the vestibular apparatus. Optimization of parameters of the optokinetic nystagmus was reflected in the criterion of positive difference + delta between angular velocities of the slow component of the nystagmus and optokinetic stimuli. It is postulated that the criterion + delta is a necessary factor in the organization of optimal time relations between optokinetic stimuli and central processes that are responsible for the final result--sensory image of movement."}
{"id": "PMID:1082533", "title": "Coronary artery bypass with freeze-preserved saphenous vein allografts.", "content": "Over the past 5 years, 13 patients had coronary artery bypass performed with freeze-preserved saphenous vein allografts. There were no operative deaths or significant morbidity. Six patients were studied postoperatively at 42, 37, 10, 7, 5, and 1 months. Six of 8 grafts were patent with good flow. There were four late deaths; two of these occurred in patients who had concomitant resection of a ventricular aneurysm. Of the 9 surviving patients, 6 (6/9) are asymptomatic and 2 (2/9) have occasional chest pains; the condition of 1 patient (1/9) is unchanged. This experience suggests that free-preserved saphenous vein allografts may be used successfully for coronary bypass when autologous veins and internal mammary arteries are unavailable or insufficient for multiple bypass.", "contents": "Coronary artery bypass with freeze-preserved saphenous vein allografts. Over the past 5 years, 13 patients had coronary artery bypass performed with freeze-preserved saphenous vein allografts. There were no operative deaths or significant morbidity. Six patients were studied postoperatively at 42, 37, 10, 7, 5, and 1 months. Six of 8 grafts were patent with good flow. There were four late deaths; two of these occurred in patients who had concomitant resection of a ventricular aneurysm. Of the 9 surviving patients, 6 (6/9) are asymptomatic and 2 (2/9) have occasional chest pains; the condition of 1 patient (1/9) is unchanged. This experience suggests that free-preserved saphenous vein allografts may be used successfully for coronary bypass when autologous veins and internal mammary arteries are unavailable or insufficient for multiple bypass."}
{"id": "PMID:1082530", "title": "[Effect of angular accelerations on the sensory and motor components of fixation eye movements].", "content": "The effect of angular accelerations on the eye fixation function and concomitant sensory reactions was investigated under special conditions. An illusion of target distortion towards the rapid component of the vestibular nystagmus developed in these situations. Changes in the eye fixation movements and \"sensation of target\" were coordinated and depended on the direction of the vestibular nystagmus. The findings indicate that during an exposure to angular accelerations the stability of target perceptions is disturbed and objectively recorded errors of its fixation by the optic apparatus are made.", "contents": "[Effect of angular accelerations on the sensory and motor components of fixation eye movements]. The effect of angular accelerations on the eye fixation function and concomitant sensory reactions was investigated under special conditions. An illusion of target distortion towards the rapid component of the vestibular nystagmus developed in these situations. Changes in the eye fixation movements and \"sensation of target\" were coordinated and depended on the direction of the vestibular nystagmus. The findings indicate that during an exposure to angular accelerations the stability of target perceptions is disturbed and objectively recorded errors of its fixation by the optic apparatus are made."}
{"id": "PMID:1082534", "title": "[Disseminated plane xanthoma with photosensitization].", "content": "A case of diffuse normolipemic plane xanthome showing no association with systemic disease (multiple myeloma, leukemia, reticulosis, or dysglobulinaemia), in a 50 year old female, is reported. The patient presented hypersensitivity to the erythrogenic spectrum with pathological response and lowered MED. The B lymphocytes in the peripherical blood was raised. The authors postulated that B lymphocytes probably play an important role in the pathogenesis of the skin light-induced reactions.", "contents": "[Disseminated plane xanthoma with photosensitization]. A case of diffuse normolipemic plane xanthome showing no association with systemic disease (multiple myeloma, leukemia, reticulosis, or dysglobulinaemia), in a 50 year old female, is reported. The patient presented hypersensitivity to the erythrogenic spectrum with pathological response and lowered MED. The B lymphocytes in the peripherical blood was raised. The authors postulated that B lymphocytes probably play an important role in the pathogenesis of the skin light-induced reactions."}
{"id": "PMID:1082537", "title": "[Collagen disease with pure red cell anemia and perimembraneous glomerulonephritis (author's transl)].", "content": "Record on a case of collagen disease with pure red cell anemia and perimembraneous glomerulonephritis. The direct antiglobulin tests with anti-IgG and anti-kappaserum were positive as were the tests on antinuclear serum-antibodies. During therapy with fluocortolon and azathioprin the anemia remitted completely even when therapy was stopped. The antiglobulin tests became negative, the glomerulitis remained active, there was no deterioration of renal function.", "contents": "[Collagen disease with pure red cell anemia and perimembraneous glomerulonephritis (author's transl)]. Record on a case of collagen disease with pure red cell anemia and perimembraneous glomerulonephritis. The direct antiglobulin tests with anti-IgG and anti-kappaserum were positive as were the tests on antinuclear serum-antibodies. During therapy with fluocortolon and azathioprin the anemia remitted completely even when therapy was stopped. The antiglobulin tests became negative, the glomerulitis remained active, there was no deterioration of renal function."}
{"id": "PMID:1082538", "title": "[Endoscopic follow-up studies of erosions of the gastric mucosa (author's transl].", "content": "From our endoscopic follow-up studies in 65 patients we are inclined to think that the various forms of erosive changes of the gastric mucosa represent different developmental stages of the same pathological process, in which the final stage may possibly be a hyperplasia of mucosal folds with gastro-intestinal loss of protein. A change into a genuine polyposis or even the development of a polypous carcinoma, as suggested by the information of Nakamura, was not observed by us, but the observation time so far has probably been too short for this anyway.", "contents": "[Endoscopic follow-up studies of erosions of the gastric mucosa (author's transl]. From our endoscopic follow-up studies in 65 patients we are inclined to think that the various forms of erosive changes of the gastric mucosa represent different developmental stages of the same pathological process, in which the final stage may possibly be a hyperplasia of mucosal folds with gastro-intestinal loss of protein. A change into a genuine polyposis or even the development of a polypous carcinoma, as suggested by the information of Nakamura, was not observed by us, but the observation time so far has probably been too short for this anyway."}
{"id": "PMID:1082539", "title": "Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA.", "content": "The DNA of bacteriophage T5+ (molecular weight 76 X 10(6) dalton) has been dissected by various specific endonucleases. The restriction enzymes HindIII and EcoRI produce 16 and 7 fragments respectively, whereas Sal and Sma produce 4 fragments each. Complete cleavage maps were established for the enzymes EcoRI, Sal and Sma and an almost complete map for HindIII. Furthermore the location and size of the deletions St 20, St 14, b3, St 0 and b1 were determined. The correlation of the genetic and functional map of the phage with the arrangement of fragments produced by the different enzymes has been established.", "contents": "Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA. The DNA of bacteriophage T5+ (molecular weight 76 X 10(6) dalton) has been dissected by various specific endonucleases. The restriction enzymes HindIII and EcoRI produce 16 and 7 fragments respectively, whereas Sal and Sma produce 4 fragments each. Complete cleavage maps were established for the enzymes EcoRI, Sal and Sma and an almost complete map for HindIII. Furthermore the location and size of the deletions St 20, St 14, b3, St 0 and b1 were determined. The correlation of the genetic and functional map of the phage with the arrangement of fragments produced by the different enzymes has been established."}
{"id": "PMID:1082540", "title": "Comparison of cell-mediated immune reactions in sympathetic and phacogenic ophthalmia with reactions in uveitis of other origin.", "content": "Cell-mediated immunity was studied in three cases of genuine sympathetic ophthalmia, i.e. in cases without lens involvement. In control experiments, lymphocyte transformation tests (uptake of 3H-thymidine) and leukocyte migration inhibition tests (Soborg-Bendixen) gave similar results for six normal persons, six patients with uveitis and two cases of phacogenic ophthalmia. In contrast, two cases of genuine sympathetic ophthamlia, namely those with recent enucleation, showed a significantly increased lymphocyte stimulation index when tested with homologous retina, not with choroid.", "contents": "Comparison of cell-mediated immune reactions in sympathetic and phacogenic ophthalmia with reactions in uveitis of other origin. Cell-mediated immunity was studied in three cases of genuine sympathetic ophthalmia, i.e. in cases without lens involvement. In control experiments, lymphocyte transformation tests (uptake of 3H-thymidine) and leukocyte migration inhibition tests (Soborg-Bendixen) gave similar results for six normal persons, six patients with uveitis and two cases of phacogenic ophthalmia. In contrast, two cases of genuine sympathetic ophthamlia, namely those with recent enucleation, showed a significantly increased lymphocyte stimulation index when tested with homologous retina, not with choroid."}
{"id": "PMID:1082541", "title": "Lymphocytic choriomeningitis virus-induced ocular immunopathology.", "content": "Lymphocytic choriomeningitis (LCM) virus of the mice provides perhaps the most extensively studied model of viral-induced immunopathology. Intraocular inoculation of this virus in the adult mouse produces a severe and progressive uveitis with involvement also of the cornea and retina. Intracerebral infection of the newborn rat with LCM virus produces a severe retinopathy, with only minimal involvement of the uveal tract. We review in this paper recent studies on these two animal models of LCM virus disease which demonstrate: (1) the histopathology of the ocular lesion; (2) that immunosuppression after infection prevents development of the disease; (3) that passive transfer of sensitized lymphoid cells to the immunosuppressed infected animal can then trigger ocular disease, and (4) that T lymphocytes, and not circulating antibody, play the major role in the pathogenesis of this disease process.", "contents": "Lymphocytic choriomeningitis virus-induced ocular immunopathology. Lymphocytic choriomeningitis (LCM) virus of the mice provides perhaps the most extensively studied model of viral-induced immunopathology. Intraocular inoculation of this virus in the adult mouse produces a severe and progressive uveitis with involvement also of the cornea and retina. Intracerebral infection of the newborn rat with LCM virus produces a severe retinopathy, with only minimal involvement of the uveal tract. We review in this paper recent studies on these two animal models of LCM virus disease which demonstrate: (1) the histopathology of the ocular lesion; (2) that immunosuppression after infection prevents development of the disease; (3) that passive transfer of sensitized lymphoid cells to the immunosuppressed infected animal can then trigger ocular disease, and (4) that T lymphocytes, and not circulating antibody, play the major role in the pathogenesis of this disease process."}
{"id": "PMID:1082542", "title": "Nonspecific immune parameters and hepatitis B antigens in patients with uveitis.", "content": "In the sera of 217 patients with uveitis the immunoglobulins IgG, IgA, IgM as well as beta1A (a measurable metabolic product of the third component of complement) were measured by radial immunodiffusion. IgG was below normal levels in 12% of the patients, IgM was increased in 12%, while reduced beta1A concentrations could be found in 28%. 95 sera were also tested for the presence of antibodies against cell nuclei, smooth muscle and thyroglobulin. There was no significantly increased incidence of any of these antibodies. The data were interpreted as not being in favor of the hypothesis that autoimmune phenomena play a key role in the pathogenesis of uveitis. An unexpected finding was the high frequency of hepatitis B antigen (13%) and corresponding antibodies (23%) in sera of uveitis patients compared to other hospitalized patients and blood donors. At present, there is no explanation for this phenomenon.", "contents": "Nonspecific immune parameters and hepatitis B antigens in patients with uveitis. In the sera of 217 patients with uveitis the immunoglobulins IgG, IgA, IgM as well as beta1A (a measurable metabolic product of the third component of complement) were measured by radial immunodiffusion. IgG was below normal levels in 12% of the patients, IgM was increased in 12%, while reduced beta1A concentrations could be found in 28%. 95 sera were also tested for the presence of antibodies against cell nuclei, smooth muscle and thyroglobulin. There was no significantly increased incidence of any of these antibodies. The data were interpreted as not being in favor of the hypothesis that autoimmune phenomena play a key role in the pathogenesis of uveitis. An unexpected finding was the high frequency of hepatitis B antigen (13%) and corresponding antibodies (23%) in sera of uveitis patients compared to other hospitalized patients and blood donors. At present, there is no explanation for this phenomenon."}
{"id": "PMID:1082545", "title": "Effect of levamisole on E-rosette-forming cells in vivo and in vitro in Hodgkin's disease.", "content": "Incubation in vitro of lymphocytes from patients with Hodgkin's disease with 40 mug per milliliter of levamisole resulted in a rise in the number of E-rosette-forming cells from 33.6 +/- 12.5 per cent (mean +/- S.D.) to 56.7 +/- 14.6 per cent. The drug had no effect on normal lymphocytes. Ten patients with Hodgkin's disease treated six months previously with levamisole were restudied. The positive skin tests to PPD, candida and mumps persisted. However, the E-rosette-forming cells decreased to the pretreatment levels (34.7 +/- 6.4 per cent). Readministration of 150 mg of levamisole for three days raised the number of E-rosette-forming cells to 54.1 +/- 5.6 per cent. This effect was observed for at least two months. However, the drug had no effect in vitro as long as the in vivo effect persisted. These results demonstrate a clear immunologic effect of levamisole in Hodgkin's disease and indicate that the low number of E-rosette-forming cells is not due to a real T-cell depletion.", "contents": "Effect of levamisole on E-rosette-forming cells in vivo and in vitro in Hodgkin's disease. Incubation in vitro of lymphocytes from patients with Hodgkin's disease with 40 mug per milliliter of levamisole resulted in a rise in the number of E-rosette-forming cells from 33.6 +/- 12.5 per cent (mean +/- S.D.) to 56.7 +/- 14.6 per cent. The drug had no effect on normal lymphocytes. Ten patients with Hodgkin's disease treated six months previously with levamisole were restudied. The positive skin tests to PPD, candida and mumps persisted. However, the E-rosette-forming cells decreased to the pretreatment levels (34.7 +/- 6.4 per cent). Readministration of 150 mg of levamisole for three days raised the number of E-rosette-forming cells to 54.1 +/- 5.6 per cent. This effect was observed for at least two months. However, the drug had no effect in vitro as long as the in vivo effect persisted. These results demonstrate a clear immunologic effect of levamisole in Hodgkin's disease and indicate that the low number of E-rosette-forming cells is not due to a real T-cell depletion."}
{"id": "PMID:1082556", "title": "Characterization of thymus- and bone marrow-derived lymphocytes in rats by means of 3H-uridine incorporation.", "content": "Lymphocytes from various lymphoid organs and of the thoracic duct of normal and thymectomized rats, irradiated and reconstituted with syngeneic bone marrow were tested in vitro in a minimal non-enriched cultivation medium with 3H-uridine, and the percentage of uridine-labeled lymphocytes was determined. The highest number of heavily labeled small lymphocytes was found in the thymus and the thoracic duct, less in peripheral blood, the lymph nodes and the spleen, and the smallest numbers in the bone marrow. A reduced ability of uridine uptake was noted in the thymectomized animals. The method of immune rosette formation was used to determine the presence of B lymphocytes in the lymphoid rat population. The highest quantity of B lymphocytes was noted in bone marrow and the least in the thymus and the thoracic duct. Thymectomized animals had a significantly higher percentage of EAC rosettes than normal and sham-operated animals. The methods employed and existing literary data enabled us to identify the heavily uridine-labeled lymphocytes as T cells, while unlabeled lymphocytes are considered to be B cells. The difference in uriding uptake by rat lymphocytes may serve as one of the T lymphocyte markers in a heterologous lymphoid population.", "contents": "Characterization of thymus- and bone marrow-derived lymphocytes in rats by means of 3H-uridine incorporation. Lymphocytes from various lymphoid organs and of the thoracic duct of normal and thymectomized rats, irradiated and reconstituted with syngeneic bone marrow were tested in vitro in a minimal non-enriched cultivation medium with 3H-uridine, and the percentage of uridine-labeled lymphocytes was determined. The highest number of heavily labeled small lymphocytes was found in the thymus and the thoracic duct, less in peripheral blood, the lymph nodes and the spleen, and the smallest numbers in the bone marrow. A reduced ability of uridine uptake was noted in the thymectomized animals. The method of immune rosette formation was used to determine the presence of B lymphocytes in the lymphoid rat population. The highest quantity of B lymphocytes was noted in bone marrow and the least in the thymus and the thoracic duct. Thymectomized animals had a significantly higher percentage of EAC rosettes than normal and sham-operated animals. The methods employed and existing literary data enabled us to identify the heavily uridine-labeled lymphocytes as T cells, while unlabeled lymphocytes are considered to be B cells. The difference in uriding uptake by rat lymphocytes may serve as one of the T lymphocyte markers in a heterologous lymphoid population."}
{"id": "PMID:1082558", "title": "[Ventriculography with methylglucamine iocarmate (Dimer-X). Experimental and Clinical study (author's transl)].", "content": "Evaluation of 60% solution of Methylglucamine iocarmate (Dimer-X) for ventriculography in animal experiment and clinical cases was reported. The experimental result was compared with that of Conray 60 and Angiografin, which was reported in our previous papers. In seven intraventricular injections of 2.0 ml, administered to 6 dogs, convulsion was observed on three occasions, facial myoclonus on 1 and no symptoms on the remaining three. However, in 7 intraventricular injections of 1.5 ml for 6 dogs, 6 were symptom-free and only residual one showed convulsion. And no dog died in either experiment. No changes were seen in a subsequent ventriculogram one month after the 1st ventriculography with Dimer-X and also no histological changes of the ventricular wall were revealed one month after the first experiment. These experimental results suggested less toxicity of Dimer-X than Conray 60 or Angiografin. Clinically, in 17 neurosurgical patients, ventriculography was performed with 1-5 ml of Dimer-X. No patients showed convulsive complication, but there was one episode of vomiting in each of 2 cases and slight headache in one case. From our experimental and clinical studies, it is concluded that the Dimer-X is to be considered the best watersoluble contrast medium for ventriculography at present.", "contents": "[Ventriculography with methylglucamine iocarmate (Dimer-X). Experimental and Clinical study (author's transl)]. Evaluation of 60% solution of Methylglucamine iocarmate (Dimer-X) for ventriculography in animal experiment and clinical cases was reported. The experimental result was compared with that of Conray 60 and Angiografin, which was reported in our previous papers. In seven intraventricular injections of 2.0 ml, administered to 6 dogs, convulsion was observed on three occasions, facial myoclonus on 1 and no symptoms on the remaining three. However, in 7 intraventricular injections of 1.5 ml for 6 dogs, 6 were symptom-free and only residual one showed convulsion. And no dog died in either experiment. No changes were seen in a subsequent ventriculogram one month after the 1st ventriculography with Dimer-X and also no histological changes of the ventricular wall were revealed one month after the first experiment. These experimental results suggested less toxicity of Dimer-X than Conray 60 or Angiografin. Clinically, in 17 neurosurgical patients, ventriculography was performed with 1-5 ml of Dimer-X. No patients showed convulsive complication, but there was one episode of vomiting in each of 2 cases and slight headache in one case. From our experimental and clinical studies, it is concluded that the Dimer-X is to be considered the best watersoluble contrast medium for ventriculography at present."}
{"id": "PMID:1082559", "title": "Comparing Evans' index and computerized axial tomography in assessing relationship of ventricular size to brain size.", "content": "Using pneumoencephalography and computerized axial tomography (EMI scanning) Polaroid pictures, the relationship between ventricular size and cerebral size was investigated in 35 patients. Evans' index was used for pneumoencephalograms, and planimetric measurement of the ventricular and cerebral cross-sectional areas was used for EMI scanning. The percentage ratio for the latter technique is termed VBR. The correlation coefficient between the two methods was 0.9510 (p less than 0.001).", "contents": "Comparing Evans' index and computerized axial tomography in assessing relationship of ventricular size to brain size. Using pneumoencephalography and computerized axial tomography (EMI scanning) Polaroid pictures, the relationship between ventricular size and cerebral size was investigated in 35 patients. Evans' index was used for pneumoencephalograms, and planimetric measurement of the ventricular and cerebral cross-sectional areas was used for EMI scanning. The percentage ratio for the latter technique is termed VBR. The correlation coefficient between the two methods was 0.9510 (p less than 0.001)."}
{"id": "PMID:1082560", "title": "Dynamic cisternography in normal dogs and in human beings.", "content": "Cisternography and ventriculography were performed in five normal anesthetized dogs to study the dynamics of tracer dispersal. Indium 111-diethylenetriaminepentaacetic acid was injected into lateral ventricles, cisterna magna, thoracic subarachnoid space, and lumbar sac. The patterns of dispersal from each injection site support a nonflow dispersal mechanism with a tendency toward ultimate equilibrium distribution throughout the cerebrospinal fluid. Quantitative data in humans complement observations made in dogs. A theory of abnormal tracer movements, based on factors observed to affect tracer dispersal in dog and man, is proposed and applied to the interpretation of abnormal cisternographic patterns seen in clinical situations.", "contents": "Dynamic cisternography in normal dogs and in human beings. Cisternography and ventriculography were performed in five normal anesthetized dogs to study the dynamics of tracer dispersal. Indium 111-diethylenetriaminepentaacetic acid was injected into lateral ventricles, cisterna magna, thoracic subarachnoid space, and lumbar sac. The patterns of dispersal from each injection site support a nonflow dispersal mechanism with a tendency toward ultimate equilibrium distribution throughout the cerebrospinal fluid. Quantitative data in humans complement observations made in dogs. A theory of abnormal tracer movements, based on factors observed to affect tracer dispersal in dog and man, is proposed and applied to the interpretation of abnormal cisternographic patterns seen in clinical situations."}
{"id": "PMID:1082562", "title": "[A radioautographic study of the dynamics of 3H-thymidine incorporation into the limb tissues of anuran amphibians during the process of loss of regenerating capacity].", "content": "DNA synthesis was studied by 3H-thymidine incorporation in limb tissues of mesodermal origin in metamorphosing common frog (Rana temporaria) tadpoles in the process of loss of regenerative ability. During metamorphosis of tadpoles, the process of histodifferentiation is accompanied by the decrease in the number of nuclei incorporating 3H-thymidine. In tadpoles capable of regeneration, during the first days after limb amputation the index of labelled nuclei decreases, as compared with that in intact tissues, and then, as the regenerate forms, increases. In tadpoles uncapable of regeneration, the index of labelled nuclei after limb amputation decreases in the stump tissues during the whole experiment but is high enough in the scar connective tissue cells. Thus, the loss of regenerative ability of anuran limbs appears to be related with the decrease in the number of DNA-synthesizing cells in tissues of mesodermal origin, due apparently to the progress of their differentiation", "contents": "[A radioautographic study of the dynamics of 3H-thymidine incorporation into the limb tissues of anuran amphibians during the process of loss of regenerating capacity]. DNA synthesis was studied by 3H-thymidine incorporation in limb tissues of mesodermal origin in metamorphosing common frog (Rana temporaria) tadpoles in the process of loss of regenerative ability. During metamorphosis of tadpoles, the process of histodifferentiation is accompanied by the decrease in the number of nuclei incorporating 3H-thymidine. In tadpoles capable of regeneration, during the first days after limb amputation the index of labelled nuclei decreases, as compared with that in intact tissues, and then, as the regenerate forms, increases. In tadpoles uncapable of regeneration, the index of labelled nuclei after limb amputation decreases in the stump tissues during the whole experiment but is high enough in the scar connective tissue cells. Thus, the loss of regenerative ability of anuran limbs appears to be related with the decrease in the number of DNA-synthesizing cells in tissues of mesodermal origin, due apparently to the progress of their differentiation"}
{"id": "PMID:1082563", "title": "[A radioautographic study of changes in the intensity of total protein synthesis in the limb tissues of anuran amphibia during loss of regenerating capacity].", "content": "The intensity of total protein synthesis was estimated by 3H-lysine incorporation in the hind limb tissues of mesodermal origin in the common frog tadpoles. In the course of limb development and loss of regenerative ability, the intensity of 3H-lysine incorporation in the differentiating muscle tissue and cartilage epiphysis increased; the cartilage diaphysis is characterized by the low level of 3H-lysine incorporation. At the stage Ia of regeneration after the limb amputation the intensity of labeling in the tissues of organ residue descreases until the stage of regeneration blastema formation and then, during the formation of limb rudiment, rapidly increases. The blastema cells are characterized by the high intensity of 3H-lysine incorporation. At the stage IIIa of wound healing after the limb amputation, the intensity of 3H-lysine incorporation in the differentiated muscles and cartilage epiphysis gradually decreases. The intensity of labeling in the scar cells is high enough.", "contents": "[A radioautographic study of changes in the intensity of total protein synthesis in the limb tissues of anuran amphibia during loss of regenerating capacity]. The intensity of total protein synthesis was estimated by 3H-lysine incorporation in the hind limb tissues of mesodermal origin in the common frog tadpoles. In the course of limb development and loss of regenerative ability, the intensity of 3H-lysine incorporation in the differentiating muscle tissue and cartilage epiphysis increased; the cartilage diaphysis is characterized by the low level of 3H-lysine incorporation. At the stage Ia of regeneration after the limb amputation the intensity of labeling in the tissues of organ residue descreases until the stage of regeneration blastema formation and then, during the formation of limb rudiment, rapidly increases. The blastema cells are characterized by the high intensity of 3H-lysine incorporation. At the stage IIIa of wound healing after the limb amputation, the intensity of 3H-lysine incorporation in the differentiated muscles and cartilage epiphysis gradually decreases. The intensity of labeling in the scar cells is high enough."}
{"id": "PMID:1082564", "title": "[Cell population kinetics during retinal histogenesis in Rana temporaria frogs].", "content": "The histogenesis of retina was studied by means of 3H-thymidine autoradiography in the Rana temporaria embryos at the 17-25th developmental stages. At all these stages the central zone of retina is more differentiated than the peripheral one. The index of labeled nuclei in the central zone changes from 74-80 to 1.5-2.0% and in the peripheral zone from 74-80 to 60%. The outcome of the first cells from the reproduction cycle is realized beginning from the moment of the optic vesicle invagination. During the histogenesis of retina in anurans both the reproduction and the onset of differentiation of different cell types take place. The heterogeneity of the population of proliferating cells at all the stages studied proved to be similar. The data obtained are compared with those characterizing the kinetics of cell populations during the histogenesis of retina in avian and mammal embryos.", "contents": "[Cell population kinetics during retinal histogenesis in Rana temporaria frogs]. The histogenesis of retina was studied by means of 3H-thymidine autoradiography in the Rana temporaria embryos at the 17-25th developmental stages. At all these stages the central zone of retina is more differentiated than the peripheral one. The index of labeled nuclei in the central zone changes from 74-80 to 1.5-2.0% and in the peripheral zone from 74-80 to 60%. The outcome of the first cells from the reproduction cycle is realized beginning from the moment of the optic vesicle invagination. During the histogenesis of retina in anurans both the reproduction and the onset of differentiation of different cell types take place. The heterogeneity of the population of proliferating cells at all the stages studied proved to be similar. The data obtained are compared with those characterizing the kinetics of cell populations during the histogenesis of retina in avian and mammal embryos."}
{"id": "PMID:1082568", "title": "[So-called \"pseudorheumatoid\" nodules in childhood (author's transl)].", "content": "This report concerns 4 patients, 2 to 2,5 years of age, with subcutaneous \"pseudorheumatoid\" nodules in the occipital region. None of the children suffered from any real rheumatic or rheumatoid disease, nor were there any biochemical signs of those diseases detectable. The course of the nodules was benign, they disappeared within several years, and no later rheumatic disease occurred. Extirpation and histology was performed in all 4 cases, the microscopic diagnosis being uniformly \"rheumatic granuloma\". The own observations are confronted with the literature. It is concluded that, inspite of the histological similarity of true rheumatic granuloma with the \"pseudorheumatic\" nodules, no etiologic relations between the two conditions seem to exist. The benignity of the pseudorheumatic nodules is underlined, and the diagnostic and prognostic importance of the condition is stressed.", "contents": "[So-called \"pseudorheumatoid\" nodules in childhood (author's transl)]. This report concerns 4 patients, 2 to 2,5 years of age, with subcutaneous \"pseudorheumatoid\" nodules in the occipital region. None of the children suffered from any real rheumatic or rheumatoid disease, nor were there any biochemical signs of those diseases detectable. The course of the nodules was benign, they disappeared within several years, and no later rheumatic disease occurred. Extirpation and histology was performed in all 4 cases, the microscopic diagnosis being uniformly \"rheumatic granuloma\". The own observations are confronted with the literature. It is concluded that, inspite of the histological similarity of true rheumatic granuloma with the \"pseudorheumatic\" nodules, no etiologic relations between the two conditions seem to exist. The benignity of the pseudorheumatic nodules is underlined, and the diagnostic and prognostic importance of the condition is stressed."}
{"id": "PMID:1082569", "title": "[The presence of cold agglutinins in hemolytic uremic syndrome (author's transl)].", "content": "A boy, 2 years old, developed a HUS after a pneumonitis. He was treated with Heparin, salicylates and recurrent peritoneal dialysis and recovered slowly. The course of the disease was complicated by myocarditis, gastric hemorrhage and severe neurologic disturbances. 7 days after unset of hemolysis a cold agglutinin titer of 1:256 was detected. This fact arises the question whether infection with Mycoplasma pneumoniae and the presence of cold agglutinins in serum could be involved in the development of HUS. The possibility of a viral etiology for this disease is discussed.", "contents": "[The presence of cold agglutinins in hemolytic uremic syndrome (author's transl)]. A boy, 2 years old, developed a HUS after a pneumonitis. He was treated with Heparin, salicylates and recurrent peritoneal dialysis and recovered slowly. The course of the disease was complicated by myocarditis, gastric hemorrhage and severe neurologic disturbances. 7 days after unset of hemolysis a cold agglutinin titer of 1:256 was detected. This fact arises the question whether infection with Mycoplasma pneumoniae and the presence of cold agglutinins in serum could be involved in the development of HUS. The possibility of a viral etiology for this disease is discussed."}
{"id": "PMID:1082570", "title": "[Old and new aspects of childhood tuberculosis (author's transl)].", "content": "During the preceding 8 years 50 children were treated for active tuberculosis at the University of Innsbruck Department of Pediatrics. None of them were vaccinated against tuberculosis. Three children died; one of them from a fulminant miliary tuberculosis, two others from tuberculous meningitis; both of them were neurosurgically explored due to massive increase of intracranial pressure before a definite diagnosis was established. Two other patients, out of eleven with Tb-meningitis, are suffering permanent damages. Each one of the fatal cases and those with permanent sequelae could have been avoided by early diagnosis, which would have been possible in every case. It's our impression, that, with the decrease in the number of infected patients, there is also a decrease in the awareness and knowledge of this disease. We found the same to be true for the performance and interpretation of the Tb testing. Newborns immunized with BCG have to be tested for a positive tuberculine conversion to a dilution of Mantoux 1:100, preferably 3 months after the vaccination. Children with negative delayed sensitivity to tuberculin have to be reimmunized.", "contents": "[Old and new aspects of childhood tuberculosis (author's transl)]. During the preceding 8 years 50 children were treated for active tuberculosis at the University of Innsbruck Department of Pediatrics. None of them were vaccinated against tuberculosis. Three children died; one of them from a fulminant miliary tuberculosis, two others from tuberculous meningitis; both of them were neurosurgically explored due to massive increase of intracranial pressure before a definite diagnosis was established. Two other patients, out of eleven with Tb-meningitis, are suffering permanent damages. Each one of the fatal cases and those with permanent sequelae could have been avoided by early diagnosis, which would have been possible in every case. It's our impression, that, with the decrease in the number of infected patients, there is also a decrease in the awareness and knowledge of this disease. We found the same to be true for the performance and interpretation of the Tb testing. Newborns immunized with BCG have to be tested for a positive tuberculine conversion to a dilution of Mantoux 1:100, preferably 3 months after the vaccination. Children with negative delayed sensitivity to tuberculin have to be reimmunized."}
{"id": "PMID:1082571", "title": "Alpha1-antitrypsin deficiency and liver disease in children: phenotypes, manifestations, and prognosis.", "content": "Among 424 children with liver disease, 20 had alpha1-antitrypsin deficiency associated with protease inhibitor ZZ phenotype. This disorder manifested itself as cholestasis in early infancy in 19 children. Jaundice and pruritus cleared in 16 of these by 7 months of age, but hepatomegaly and laboratory evidence of mild hepatic dysfunction persisted in all. Biliary cirrhosis and portal hypertension eventually developed or was suspected in eight, and hypoplasia of intraheptic bile ducts was demonstrated in another four. Routine screening revealed intermediate alpha1-antitrypsin deficiency in 16 other children with various types of liver disease. The phenotype in these patients was MZ, MS, or SZ. PAS-positive granules were present in liver of all patients with the ZZ phenotype and in none with other phenotypes. The findings indicate that manifestations and prognosis of this inherited liver disease are extremely variable.", "contents": "Alpha1-antitrypsin deficiency and liver disease in children: phenotypes, manifestations, and prognosis. Among 424 children with liver disease, 20 had alpha1-antitrypsin deficiency associated with protease inhibitor ZZ phenotype. This disorder manifested itself as cholestasis in early infancy in 19 children. Jaundice and pruritus cleared in 16 of these by 7 months of age, but hepatomegaly and laboratory evidence of mild hepatic dysfunction persisted in all. Biliary cirrhosis and portal hypertension eventually developed or was suspected in eight, and hypoplasia of intraheptic bile ducts was demonstrated in another four. Routine screening revealed intermediate alpha1-antitrypsin deficiency in 16 other children with various types of liver disease. The phenotype in these patients was MZ, MS, or SZ. PAS-positive granules were present in liver of all patients with the ZZ phenotype and in none with other phenotypes. The findings indicate that manifestations and prognosis of this inherited liver disease are extremely variable."}
{"id": "PMID:1082572", "title": "Membranoproliferative glomerulonephritis in childhood cirrhosis associated with alpha1-antitrypsin deficiency.", "content": "Three alpha1-antitrypsin (alpha1AT) deficient, protease inhibitor type ZZ children who died from cirrhosis and its complications had membranoproliferative glomerulonephritis at postmortem examination. During life, all three had clinical and laboratory evidence of renal disease which became apparent when hepatic decompensation developed. Immunofluorescence studies and electron microscopy performed in one patient revealed subendothelial deposits of alpha1AT, complement, and immune globulins along the glomerular basement membrane. The pathogenesis of these renal lesions is speculative. Glomerular lesions were not observed in kidney sections of 16 children who died from cirrhosis but who were not alpha1AT-deficient. The present study suggests that renal involvement may be yet another manifestation of disease associated with alpha1AT deficiency.", "contents": "Membranoproliferative glomerulonephritis in childhood cirrhosis associated with alpha1-antitrypsin deficiency. Three alpha1-antitrypsin (alpha1AT) deficient, protease inhibitor type ZZ children who died from cirrhosis and its complications had membranoproliferative glomerulonephritis at postmortem examination. During life, all three had clinical and laboratory evidence of renal disease which became apparent when hepatic decompensation developed. Immunofluorescence studies and electron microscopy performed in one patient revealed subendothelial deposits of alpha1AT, complement, and immune globulins along the glomerular basement membrane. The pathogenesis of these renal lesions is speculative. Glomerular lesions were not observed in kidney sections of 16 children who died from cirrhosis but who were not alpha1AT-deficient. The present study suggests that renal involvement may be yet another manifestation of disease associated with alpha1AT deficiency."}
{"id": "PMID:1082573", "title": "Frequency of detection of Hemophilus influenzae type b capsular polysaccharide in infants and children with pneumonia.", "content": "Countercurrent immunoelectrophoresis was used to detect H. influenzae type b antigen in sera of 65 consecutive infants and children with clinical and radiographic evidence of pneumonia. Antigenemia was observed in only one patient who also had H. influenzae type b bacteremia. Blood cultures from four other children were positive for H. aphrophilus (one), Streptococcus pneumoniae (two), and an alpha-hemolytic Streptococcus (one). The frequency of pharyngeal colonization with H. influenzae type b was similar in children with or without pneumonia. If H. influenzae type b was the cause of pneumonitis in the children studied, it occurred without bacteremia or antigenemia.", "contents": "Frequency of detection of Hemophilus influenzae type b capsular polysaccharide in infants and children with pneumonia. Countercurrent immunoelectrophoresis was used to detect H. influenzae type b antigen in sera of 65 consecutive infants and children with clinical and radiographic evidence of pneumonia. Antigenemia was observed in only one patient who also had H. influenzae type b bacteremia. Blood cultures from four other children were positive for H. aphrophilus (one), Streptococcus pneumoniae (two), and an alpha-hemolytic Streptococcus (one). The frequency of pharyngeal colonization with H. influenzae type b was similar in children with or without pneumonia. If H. influenzae type b was the cause of pneumonitis in the children studied, it occurred without bacteremia or antigenemia."}
{"id": "PMID:1082574", "title": "Time course of regeneration of minced frog muscles estimated by the level of energetic substrates.", "content": "1. Gastrocnemius muscles of frogs have been excised, chopped and put back into the leg. 2. The weight of the implanted muscles increases until the 8th day after the operation and then decreases to 1/3 of that of the control by the 30th day. 3. The inulin space is twice as high in regenerating muscle as in the unoperated controls. 4. Adenosine nucleotides are in an enzymatic equilibrium set by the ratio creatine/phosphorylcreatine during regeneration of the muscle. 5. Glycolytic intermediates (hexose monophosphate, fructose-1,6-diphosphate, alpha-glycerophosphate and lactate) have been measured during the course of muscle regeneration up to the 30th day. The results suggest that the glycolysis of regenerating muscle is very active. 6. The total creatine (sum of creatine and phosphorylcreatine) is very low after the operation: 5% of that of the controls; it rises sharply after the 8th day, and reaches 30% of that of the control on the 30th day. 7. The amount of total creatine seems proportional to the progress of the muscle regeneration. It is suggested to use this substance as a biochemical reference for biochemical works on muscle regeneration.", "contents": "Time course of regeneration of minced frog muscles estimated by the level of energetic substrates. 1. Gastrocnemius muscles of frogs have been excised, chopped and put back into the leg. 2. The weight of the implanted muscles increases until the 8th day after the operation and then decreases to 1/3 of that of the control by the 30th day. 3. The inulin space is twice as high in regenerating muscle as in the unoperated controls. 4. Adenosine nucleotides are in an enzymatic equilibrium set by the ratio creatine/phosphorylcreatine during regeneration of the muscle. 5. Glycolytic intermediates (hexose monophosphate, fructose-1,6-diphosphate, alpha-glycerophosphate and lactate) have been measured during the course of muscle regeneration up to the 30th day. The results suggest that the glycolysis of regenerating muscle is very active. 6. The total creatine (sum of creatine and phosphorylcreatine) is very low after the operation: 5% of that of the controls; it rises sharply after the 8th day, and reaches 30% of that of the control on the 30th day. 7. The amount of total creatine seems proportional to the progress of the muscle regeneration. It is suggested to use this substance as a biochemical reference for biochemical works on muscle regeneration."}
{"id": "PMID:1082575", "title": "[Acute pericarditis. Study of 50 consecutive cases].", "content": "50 consecutive cases of pericarditis were studied. Idiopathic and viral pericarditis made up 36 p. cent of all cases. The occurrence of tuberculous or rheumatic conditions was even lower than that reported in similar series in the literature. Amongst the clinical signs of these diseases, emphasis should be placed upon the frequency of chest pain which was increased by deep inspiration in only three out of four cases. Circulatory problems associated with the pericarditis were, on the whole, minimal. However, a fall in blood pressure was seen in 6 cases and true collapse in 3 more. The electrocardiogram showed, in addition to the classical signs, sagging of the PR interval in 36 p. cent and transient atrial fibrillation in 4 cases. As far as biological tests are concerned, it should be noted that elevation of creatine phospho-kinase to levels 4 times greater than normal may be seen, creating problems with the differential diagnosis from myocardial infarction. The course was in general favourable though two deaths occurred in this series (4 p. cent), one with tuberculous pericarditis and the other with idiopathic pericarditis.", "contents": "[Acute pericarditis. Study of 50 consecutive cases]. 50 consecutive cases of pericarditis were studied. Idiopathic and viral pericarditis made up 36 p. cent of all cases. The occurrence of tuberculous or rheumatic conditions was even lower than that reported in similar series in the literature. Amongst the clinical signs of these diseases, emphasis should be placed upon the frequency of chest pain which was increased by deep inspiration in only three out of four cases. Circulatory problems associated with the pericarditis were, on the whole, minimal. However, a fall in blood pressure was seen in 6 cases and true collapse in 3 more. The electrocardiogram showed, in addition to the classical signs, sagging of the PR interval in 36 p. cent and transient atrial fibrillation in 4 cases. As far as biological tests are concerned, it should be noted that elevation of creatine phospho-kinase to levels 4 times greater than normal may be seen, creating problems with the differential diagnosis from myocardial infarction. The course was in general favourable though two deaths occurred in this series (4 p. cent), one with tuberculous pericarditis and the other with idiopathic pericarditis."}
{"id": "PMID:1082577", "title": "Intravascular occlusive therapy: use of interventional radiology in cancer patients.", "content": "Selective transcatheter intravascular occlusion in the treatment of cancer patients is a valuable extension of interventional diagnostic radiology. Intra-arterial embolization may be performed with various substances, including autologous clot, autologous subcutaneous tissue, Gelfoam, and stainless steel coil. Clinical applications in cancer patients include control of gastrointestinal and genitourinary hemorrhage, preoperative reduction of tumor vascularity, control of local symptoms, and therapeutic reduction of tumor bulk. The technique has been used for preoperative and palliative treatment of neoplasms of the head and neck, kidney, liver, spleen, and soft tissue and bone. Transcatheter intravascular occlusion should be performed only by radiologists experienced in angiographic techniques. Inadvertent occlusion of a normal vessel and thromboembolism are possible complications.", "contents": "Intravascular occlusive therapy: use of interventional radiology in cancer patients. Selective transcatheter intravascular occlusion in the treatment of cancer patients is a valuable extension of interventional diagnostic radiology. Intra-arterial embolization may be performed with various substances, including autologous clot, autologous subcutaneous tissue, Gelfoam, and stainless steel coil. Clinical applications in cancer patients include control of gastrointestinal and genitourinary hemorrhage, preoperative reduction of tumor vascularity, control of local symptoms, and therapeutic reduction of tumor bulk. The technique has been used for preoperative and palliative treatment of neoplasms of the head and neck, kidney, liver, spleen, and soft tissue and bone. Transcatheter intravascular occlusion should be performed only by radiologists experienced in angiographic techniques. Inadvertent occlusion of a normal vessel and thromboembolism are possible complications."}
{"id": "PMID:1082581", "title": "Isolation of alpha1-antitrypsin in a new preparative electrophoresis apparatus.", "content": "The construction and operation of an apparatus is described which enables alpha1-antitrypsin to be isolated from human serum by preparative electrophoresis in polyacrylamide gel. The initial pass through the chamber yields a fraction that is predominantly albumin and several alpha1-proteins. After removal of albumin by affinity chromatography, a second pass through the chamber separates the individual alpha1-proteins. A volume of 50 ml of serum may be accommodated by the chamber, and the recovery of activity in each step is greater than 60%. The entire procedure may be completed in 36 hrs.", "contents": "Isolation of alpha1-antitrypsin in a new preparative electrophoresis apparatus. The construction and operation of an apparatus is described which enables alpha1-antitrypsin to be isolated from human serum by preparative electrophoresis in polyacrylamide gel. The initial pass through the chamber yields a fraction that is predominantly albumin and several alpha1-proteins. After removal of albumin by affinity chromatography, a second pass through the chamber separates the individual alpha1-proteins. A volume of 50 ml of serum may be accommodated by the chamber, and the recovery of activity in each step is greater than 60%. The entire procedure may be completed in 36 hrs."}
{"id": "PMID:1082596", "title": "Nonsterilizing immunity in avian malaria: an antibody-independent phenomenon.", "content": "Agammaglobulinemic chickens died with fulminating parasitemias following infection with P. gallinaceum. When rescued from otherwise fatal infection by means of chloroquine therapy, B-cell deficient chickens resisted challenge infection with the same parasite. The data suggest that nonsterilizing immunity to malaria in chickens is B-cell independent.", "contents": "Nonsterilizing immunity in avian malaria: an antibody-independent phenomenon. Agammaglobulinemic chickens died with fulminating parasitemias following infection with P. gallinaceum. When rescued from otherwise fatal infection by means of chloroquine therapy, B-cell deficient chickens resisted challenge infection with the same parasite. The data suggest that nonsterilizing immunity to malaria in chickens is B-cell independent."}
{"id": "PMID:1082597", "title": "Effect of tilorone hydrochloride on the lymphoid and interferon responses of athymic mice.", "content": "Nude athymic mice, which lack T-lymphocytes and are unable to mount a cellular immune response, failed to develop the lymphopenia so characteristically produced by a 100 mg/kg oral dose of tilorone hydrochloride in normal mice. The antiviral activity detected in athymic mice 18 hr after a 150 mg/kg oral dose of tilorone was significant, although somewhat less than that found in treated normal mice. These findings suggest that tilorone hydrochloride has a specific effect on T-lymphocytes that accounts for its effect on cellular immunity, but that its antiviral activity is not primarily mediated by T-lymphocytes.", "contents": "Effect of tilorone hydrochloride on the lymphoid and interferon responses of athymic mice. Nude athymic mice, which lack T-lymphocytes and are unable to mount a cellular immune response, failed to develop the lymphopenia so characteristically produced by a 100 mg/kg oral dose of tilorone hydrochloride in normal mice. The antiviral activity detected in athymic mice 18 hr after a 150 mg/kg oral dose of tilorone was significant, although somewhat less than that found in treated normal mice. These findings suggest that tilorone hydrochloride has a specific effect on T-lymphocytes that accounts for its effect on cellular immunity, but that its antiviral activity is not primarily mediated by T-lymphocytes."}
{"id": "PMID:1082598", "title": "Inhibition of T-lymphocyte rosetting by HL-A alloantisera and complement.", "content": "The relationship between HL-A antigens and rosetting of sheep red blood cells (SRBC) with peripheral human lymphocytes has been investigated by incubating them with HL-A antibodies. Although sensitizing the lymphocytes with HL-A alloantisera had no effect on their ability to form rosettes with SRBC, further sensitization with C6 deficient rabbit serum as a source of early complement components inhibited the formation of rosettes with SRBC. The involvement of HL-A alloantibodies in the inhibition of rosette formation was shown first by correlating the HL-A phenotype of the lymphocytes and the HL-A specificity of the alloantisera and, second, by specifically absorbing the HL-A alloantibodies from the alloantisera. Complement was needed to inhibit rosette formation since this effect was lost when rabbit serum was treated to inactivate complement. The participation of complement's classical pathway in rosette inhibition was shown by chelating the Ca2+ ions by EGTA treatment of the C6 deficient rabbit serum. Perhaps, binding of HL-A antibodies and early complement components to the lymphocyte surface disturbs the distribution of the receptors or affects the charge of the cell membrane, thus inhibiting the rosette formation with SRBC.", "contents": "Inhibition of T-lymphocyte rosetting by HL-A alloantisera and complement. The relationship between HL-A antigens and rosetting of sheep red blood cells (SRBC) with peripheral human lymphocytes has been investigated by incubating them with HL-A antibodies. Although sensitizing the lymphocytes with HL-A alloantisera had no effect on their ability to form rosettes with SRBC, further sensitization with C6 deficient rabbit serum as a source of early complement components inhibited the formation of rosettes with SRBC. The involvement of HL-A alloantibodies in the inhibition of rosette formation was shown first by correlating the HL-A phenotype of the lymphocytes and the HL-A specificity of the alloantisera and, second, by specifically absorbing the HL-A alloantibodies from the alloantisera. Complement was needed to inhibit rosette formation since this effect was lost when rabbit serum was treated to inactivate complement. The participation of complement's classical pathway in rosette inhibition was shown by chelating the Ca2+ ions by EGTA treatment of the C6 deficient rabbit serum. Perhaps, binding of HL-A antibodies and early complement components to the lymphocyte surface disturbs the distribution of the receptors or affects the charge of the cell membrane, thus inhibiting the rosette formation with SRBC."}
{"id": "PMID:1082599", "title": "Induction of secondary cytotoxic T-lymphocytes in vitro does not require cell proliferation.", "content": "Using a mouse in vitro allograft model, evidence has been obtained that, in contrast to the accepted view, the generation of cytotoxic effector function in T-lymphocytes does not necessarily require cell division.", "contents": "Induction of secondary cytotoxic T-lymphocytes in vitro does not require cell proliferation. Using a mouse in vitro allograft model, evidence has been obtained that, in contrast to the accepted view, the generation of cytotoxic effector function in T-lymphocytes does not necessarily require cell division."}
{"id": "PMID:1082600", "title": "The effect of tryptophan and a tryptophan/5-hydroxytryptophan combination on indoles in the brains of rats fed a tryptophan deficient diet.", "content": "Rats maintained on a tryptophan deficient diet had reduced brain and serum tryptophan and brain 5-hydroxyindolacetic acid levels compared to controls. 5-Hydroxytryptophan and L-tryptophan administered to these deficient rats in a combination (5:95) produced a greater elevation of indolamines and tryptophan in the brain than either amino acid alone. In rats maintained on a normal diet the urinary output of 3-hydroxykynurenine was considerably reduced by treatment with the combination of amino acids as compared to tryptophan treatment. 5-Hydroxytryptophan reduced the induction of kynurenine synthesis in the liver produced by tryptophan, implying that it is capable of inhibiting the enzyme tryptophan pyrrolase in vivo. It is suggested that the possession by 5-hydroxytryptophan of tryptophan pyrrolase inhibitory properties may make the administration of the combination a better treatment of depressed patients exhibiting an indolamine deficit than either amino acid alone.", "contents": "The effect of tryptophan and a tryptophan/5-hydroxytryptophan combination on indoles in the brains of rats fed a tryptophan deficient diet. Rats maintained on a tryptophan deficient diet had reduced brain and serum tryptophan and brain 5-hydroxyindolacetic acid levels compared to controls. 5-Hydroxytryptophan and L-tryptophan administered to these deficient rats in a combination (5:95) produced a greater elevation of indolamines and tryptophan in the brain than either amino acid alone. In rats maintained on a normal diet the urinary output of 3-hydroxykynurenine was considerably reduced by treatment with the combination of amino acids as compared to tryptophan treatment. 5-Hydroxytryptophan reduced the induction of kynurenine synthesis in the liver produced by tryptophan, implying that it is capable of inhibiting the enzyme tryptophan pyrrolase in vivo. It is suggested that the possession by 5-hydroxytryptophan of tryptophan pyrrolase inhibitory properties may make the administration of the combination a better treatment of depressed patients exhibiting an indolamine deficit than either amino acid alone."}
{"id": "PMID:1082602", "title": "Angiography in the diagnosis and therapy of hemorrhage from the large bowel.", "content": "Angiography has added a new dimension to the management of hemorrhage from the large bowel. In patients with diverticular hemorrhage, mesenteric angiography not only localizes the bleeding site but, in addition, the bleeding can be acutely controlled with intraarterial infusion of vasopressin, making an emergency colectomy unnecessary. Similarly in patients bleeding from inflammatory bowel disease or in patients with post-operative hemorrhage, angiography provides information about the nature of the lesion and selective arterial infusions of vasopressin can control the bleeding. At times intestinal varices have angiographically been demonstrated as a potential source of rectal hemorrhage while in patients with unexplained lower gastrointestinal bleeding and repeatedly negative barium and endoscopic examinations, angiography has been valuable for the diagnosis of angiodysplasia of the colon.", "contents": "Angiography in the diagnosis and therapy of hemorrhage from the large bowel. Angiography has added a new dimension to the management of hemorrhage from the large bowel. In patients with diverticular hemorrhage, mesenteric angiography not only localizes the bleeding site but, in addition, the bleeding can be acutely controlled with intraarterial infusion of vasopressin, making an emergency colectomy unnecessary. Similarly in patients bleeding from inflammatory bowel disease or in patients with post-operative hemorrhage, angiography provides information about the nature of the lesion and selective arterial infusions of vasopressin can control the bleeding. At times intestinal varices have angiographically been demonstrated as a potential source of rectal hemorrhage while in patients with unexplained lower gastrointestinal bleeding and repeatedly negative barium and endoscopic examinations, angiography has been valuable for the diagnosis of angiodysplasia of the colon."}
{"id": "PMID:1082603", "title": "Simplified superselective catheterization of arterial bleeders.", "content": "Superselective catheterization is critical when treating acute gastrointestinal arterial bleeders. The author has been using a coaxial catheter system with excellent results. The technical aspects of two representative cases are discussed. This is a particularly valuable technique when dealing with the anomalous left gastric artery which supplies the left lobe of the liver as well as the stomach.", "contents": "Simplified superselective catheterization of arterial bleeders. Superselective catheterization is critical when treating acute gastrointestinal arterial bleeders. The author has been using a coaxial catheter system with excellent results. The technical aspects of two representative cases are discussed. This is a particularly valuable technique when dealing with the anomalous left gastric artery which supplies the left lobe of the liver as well as the stomach."}
{"id": "PMID:1082604", "title": "[Delusions of skin infestations].", "content": "Delusion of skin-parasitosis is a very characteristic disorder, and it can appear as an independent syndrome or as a concomitant phenomenon of other psychiatric illnesses. Like most delusional formations it can also be induced and appear as a symbiontic psychosis; the involutional age seems to be an essential precondition for this psychopathological phenomenon.", "contents": "[Delusions of skin infestations]. Delusion of skin-parasitosis is a very characteristic disorder, and it can appear as an independent syndrome or as a concomitant phenomenon of other psychiatric illnesses. Like most delusional formations it can also be induced and appear as a symbiontic psychosis; the involutional age seems to be an essential precondition for this psychopathological phenomenon."}
{"id": "PMID:1082615", "title": "Ultraviolet polarized light study on isolated myocardial trabeculae and skeletal fibers.", "content": "The main components of the excitable membranes are protein and lipoproteins absorbing in the ultraviolet. Contractile proteins in muscle also absorb ultraviolet light. If polarized light is used, it becomes possible to distinguish the orientation of the absorbing groups either at rest or during activity. A system of microspectrophotometry in vivo using polarized ultraviolet light has been established to study the portion of myocardial trabeculae or of skeletal fibers in the central compartment of a double sucrose gap apparatus. Thus the system also allows electrophysiological studies. On both preparations, a relation between the absorbance change at 280 nm and contractile activity has been measured. These preparations also contain a fraction of oriented compounds absorbing at 280 nm and another at 260 nm. During mechanical activity, the amount of oriented compounds increases at 280 nm and decreases at 260 nm. These changes were assigned to an increase in the ratio of oriented amino acids, tyrosine and tryptophan, and to a decrease of oriented adenosine triphosphate.", "contents": "Ultraviolet polarized light study on isolated myocardial trabeculae and skeletal fibers. The main components of the excitable membranes are protein and lipoproteins absorbing in the ultraviolet. Contractile proteins in muscle also absorb ultraviolet light. If polarized light is used, it becomes possible to distinguish the orientation of the absorbing groups either at rest or during activity. A system of microspectrophotometry in vivo using polarized ultraviolet light has been established to study the portion of myocardial trabeculae or of skeletal fibers in the central compartment of a double sucrose gap apparatus. Thus the system also allows electrophysiological studies. On both preparations, a relation between the absorbance change at 280 nm and contractile activity has been measured. These preparations also contain a fraction of oriented compounds absorbing at 280 nm and another at 260 nm. During mechanical activity, the amount of oriented compounds increases at 280 nm and decreases at 260 nm. These changes were assigned to an increase in the ratio of oriented amino acids, tyrosine and tryptophan, and to a decrease of oriented adenosine triphosphate."}
{"id": "PMID:1082620", "title": "Occult gastrointestinal bleeding due to aspirin: comparison of two compounds.", "content": "Occult gastrointestinal bleeding as measured by the faecal 51Cr-labelled red-cell loss was compared in eight healthy volunteers while on therapeutic doses of soluble aspirin and a new slow-release formulation of aspirin (Deskoval). While both preparations led to increased faecal blood loss, this was significantly less during administration of Deskoval than when soluble aspirin was taken.", "contents": "Occult gastrointestinal bleeding due to aspirin: comparison of two compounds. Occult gastrointestinal bleeding as measured by the faecal 51Cr-labelled red-cell loss was compared in eight healthy volunteers while on therapeutic doses of soluble aspirin and a new slow-release formulation of aspirin (Deskoval). While both preparations led to increased faecal blood loss, this was significantly less during administration of Deskoval than when soluble aspirin was taken."}
{"id": "PMID:1082626", "title": "[Interstitial pneumonia in children on cystostatic treatment for malignant systematic disease (author's transl)].", "content": "The radiologic appearances of interstitial pneumonia are described as they appeared in 39 children treated with immunosuppressive and cytostatic substances given for malignant systemic disease. At the time most of the children were in a stage of remission of their basic illness. In most of them there may have been a pneumocystis carinii infection. 15 children died of this pneumonia. In the majority of post-mortem examinations pneumocysts could be detected.", "contents": "[Interstitial pneumonia in children on cystostatic treatment for malignant systematic disease (author's transl)]. The radiologic appearances of interstitial pneumonia are described as they appeared in 39 children treated with immunosuppressive and cytostatic substances given for malignant systemic disease. At the time most of the children were in a stage of remission of their basic illness. In most of them there may have been a pneumocystis carinii infection. 15 children died of this pneumonia. In the majority of post-mortem examinations pneumocysts could be detected."}
{"id": "PMID:1082627", "title": "[Indications for surgery of the coronaries and its results (author's transl)].", "content": "In chronic angina pectoris surgery is indicated for all patients with bridgeable central stenoses, suitable distal vessels and patent peripheral coronary circulation, if function of the ventricle is not critically reduced. when more than one vessel is diseased and with isolated stenoses of the anterior interventricular branch of the left coronary a., it does hardly latter whether the angina pectoris can be treated conservatively or not. An absolute indication for revasculariation exists when stenoses of the main branches of the left coronary artery can be shown. Pre-infarct angina (unstable or crescendo-angina, threatened infarction) should, in younger patients, always lead to immediate diagnostic ascertainment. Where severe anatomic coronary changes are present, immediate surgery is indicated, although the real danger of infarction in such patients cannot be reliably predicated as yet. Post-infarct ventricular aneurysmus should be operated on when leftsided insufficiency increases or arrythmias cannot be controlled satisfactorily any more. Surgery can be undertaken with an acceptable risk, if patients are carefully selected. As far as we know from published statistics, the quality of life of such patients can be improved for some time. But their expectation of life after surgery cannot as yet be reliably predicted, because prospective observations of comparable groups of patients treated conservatively or surgically do not yet exist. From a prospective study of McNeer (22) it appears that in 2 randomized groups there was no significant difference in time of survival after 2 years. Neither the size of the material nor the period of follow-up are sufficient for definite evaluation.", "contents": "[Indications for surgery of the coronaries and its results (author's transl)]. In chronic angina pectoris surgery is indicated for all patients with bridgeable central stenoses, suitable distal vessels and patent peripheral coronary circulation, if function of the ventricle is not critically reduced. when more than one vessel is diseased and with isolated stenoses of the anterior interventricular branch of the left coronary a., it does hardly latter whether the angina pectoris can be treated conservatively or not. An absolute indication for revasculariation exists when stenoses of the main branches of the left coronary artery can be shown. Pre-infarct angina (unstable or crescendo-angina, threatened infarction) should, in younger patients, always lead to immediate diagnostic ascertainment. Where severe anatomic coronary changes are present, immediate surgery is indicated, although the real danger of infarction in such patients cannot be reliably predicated as yet. Post-infarct ventricular aneurysmus should be operated on when leftsided insufficiency increases or arrythmias cannot be controlled satisfactorily any more. Surgery can be undertaken with an acceptable risk, if patients are carefully selected. As far as we know from published statistics, the quality of life of such patients can be improved for some time. But their expectation of life after surgery cannot as yet be reliably predicted, because prospective observations of comparable groups of patients treated conservatively or surgically do not yet exist. From a prospective study of McNeer (22) it appears that in 2 randomized groups there was no significant difference in time of survival after 2 years. Neither the size of the material nor the period of follow-up are sufficient for definite evaluation."}
{"id": "PMID:1082628", "title": "Immunological function in patients operated on with small intestinal shunts for morbid obesity.", "content": "Obese individuals, operated on with intestinal shunts, have been investigated with regard to immunological functions. After operation strong increases in skin reactions of immediate and delayed type were observed. Analysis of total WBC and differential counts, percentage T lymphocytes, immunoglobulin concentrations, and lymphocyte reactivity yielded no explanation of the findings.", "contents": "Immunological function in patients operated on with small intestinal shunts for morbid obesity. Obese individuals, operated on with intestinal shunts, have been investigated with regard to immunological functions. After operation strong increases in skin reactions of immediate and delayed type were observed. Analysis of total WBC and differential counts, percentage T lymphocytes, immunoglobulin concentrations, and lymphocyte reactivity yielded no explanation of the findings."}
{"id": "PMID:1082629", "title": "Granulopoiesis in infantile genetic agranulocytosis. In vitro cloning of marrow cells in agar culture.", "content": "A 3-year-old boy and a 2-year-old girl with infantile genetic agranulocytosis have been studied by in vitro cloning of bone marrow cells in agar culture. The patients display a normal concentration of colony forming cells and the morphological maturation is identical with that of control marrow cultured in vitro. The marrow cells of the patients show some degree of auto-stimulation indicating that endogenous production of colony stimulating factor is operating. As an inverse relationship is expected between the peripheral neutrophil count and the percentage of marrow colony forming cells in S-phase a high percentage was expected. On the contrary, we find that the percentage of colony forming cells in S-phase is extremely low indicating a genetic unresponsiveness of granulopoietic precursor cells to feed back regulation in infantile genetic agranulocytosis.", "contents": "Granulopoiesis in infantile genetic agranulocytosis. In vitro cloning of marrow cells in agar culture. A 3-year-old boy and a 2-year-old girl with infantile genetic agranulocytosis have been studied by in vitro cloning of bone marrow cells in agar culture. The patients display a normal concentration of colony forming cells and the morphological maturation is identical with that of control marrow cultured in vitro. The marrow cells of the patients show some degree of auto-stimulation indicating that endogenous production of colony stimulating factor is operating. As an inverse relationship is expected between the peripheral neutrophil count and the percentage of marrow colony forming cells in S-phase a high percentage was expected. On the contrary, we find that the percentage of colony forming cells in S-phase is extremely low indicating a genetic unresponsiveness of granulopoietic precursor cells to feed back regulation in infantile genetic agranulocytosis."}
{"id": "PMID:1082630", "title": "The content of erythrocyte 2,3 diphosphoglycerate and adenosine-5'-triphosphate in myelomatosis and leukaemia.", "content": "Ery-DPG and thus the oxygen-releasing capacity of haemoglobin increases in meylomatosis and in chronic myelocytic and lymphocytic leukaemia at decreased concentrations of haemoglobin, to the same extent as in other types of anaemia. Consequently the need for blood transfusions is less than assumed in these disorders. Ery-ATP is considerably increased in chronic myelocytic leukaemia, the values correlating positively with the 'activity' of the disease. This finding can probably be used for diagnostic and prognostic purposes.", "contents": "The content of erythrocyte 2,3 diphosphoglycerate and adenosine-5'-triphosphate in myelomatosis and leukaemia. Ery-DPG and thus the oxygen-releasing capacity of haemoglobin increases in meylomatosis and in chronic myelocytic and lymphocytic leukaemia at decreased concentrations of haemoglobin, to the same extent as in other types of anaemia. Consequently the need for blood transfusions is less than assumed in these disorders. Ery-ATP is considerably increased in chronic myelocytic leukaemia, the values correlating positively with the 'activity' of the disease. This finding can probably be used for diagnostic and prognostic purposes."}
{"id": "PMID:1082631", "title": "Evidence of different cell populations in the mouse thymus releasing and responding to mitogenic factor.", "content": "The thymocyte population of mice treated with cortisone was examined at various times with respect to its relative capacity to be triggered to DNA synthesis by soluble phytomitogens or mitogenic factors (MF) released by lymph node cells exposed to phytomitogens. The capacity of the thymocyte population to produce MF in response to phytomitogen exposure was also examined. We found that the relative blastogenic activity of both phytomitogens and MF on thymocytes increased as the cell number of the thymus was reduced by the cortisone treatment. However, the reactivity to phytomitogens increased to a higher extent. During the subsequent regeneration of the organ the stimulability of the cells by phytomitogen decreased far below that of MF. The relative capacity of thymocytes to produce MF seemed to parallel their phytomitogen reactivities. One possible explanation of the results is that there exists one subpopulation of cells in the thymus more responsive to phytomitogens than to MF and another more responsive to MF than to phytomitogen. It is possible that the cells that are phytomitogen-responsive are those that can produce MF.", "contents": "Evidence of different cell populations in the mouse thymus releasing and responding to mitogenic factor. The thymocyte population of mice treated with cortisone was examined at various times with respect to its relative capacity to be triggered to DNA synthesis by soluble phytomitogens or mitogenic factors (MF) released by lymph node cells exposed to phytomitogens. The capacity of the thymocyte population to produce MF in response to phytomitogen exposure was also examined. We found that the relative blastogenic activity of both phytomitogens and MF on thymocytes increased as the cell number of the thymus was reduced by the cortisone treatment. However, the reactivity to phytomitogens increased to a higher extent. During the subsequent regeneration of the organ the stimulability of the cells by phytomitogen decreased far below that of MF. The relative capacity of thymocytes to produce MF seemed to parallel their phytomitogen reactivities. One possible explanation of the results is that there exists one subpopulation of cells in the thymus more responsive to phytomitogens than to MF and another more responsive to MF than to phytomitogen. It is possible that the cells that are phytomitogen-responsive are those that can produce MF."}
{"id": "PMID:1082632", "title": "Lymphocyte populations and cellular immune reactions in juvenile rheumatoid arthritis.", "content": "Ten patients with juvenile rheumatoid arthritis and age- and sex-matched healthy controls were investigated in pairs. The patients were found to have both normal proportions and normal absolute numbers of T lymphocytes, B lymphocytes, and the Fc-receptor-bearing lymphoid cells in peripheral blood. No abnormality of mitogen-induced lymphocyte transformation was observed. Lymphocyte-mediated cytotoxicity induced by phytohemagglutinin (PHA) or anti-target cell antibodies was also found to be normal. As in an earlier study, impaired delayed hypersensitivity by skin testing was observed in the patient group, thus indicating a dissociation between in vivo and in vitro parameters of lymphoid cell function.", "contents": "Lymphocyte populations and cellular immune reactions in juvenile rheumatoid arthritis. Ten patients with juvenile rheumatoid arthritis and age- and sex-matched healthy controls were investigated in pairs. The patients were found to have both normal proportions and normal absolute numbers of T lymphocytes, B lymphocytes, and the Fc-receptor-bearing lymphoid cells in peripheral blood. No abnormality of mitogen-induced lymphocyte transformation was observed. Lymphocyte-mediated cytotoxicity induced by phytohemagglutinin (PHA) or anti-target cell antibodies was also found to be normal. As in an earlier study, impaired delayed hypersensitivity by skin testing was observed in the patient group, thus indicating a dissociation between in vivo and in vitro parameters of lymphoid cell function."}
{"id": "PMID:1082633", "title": "Spontaneous rosette formation with heavily haptenated sheep erythrocytes: a marker for mouse non-T lymphocytes.", "content": "Most non-T lymphocytes in nonimmunized mice spontaneously bind heavily haptenated (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP)-sheep erythrocytes. Few if any T lymphocytes are rosette formers with such erythrocytes. The NNP rosette formation is suggested to be a marker for mouse non-T cells.", "contents": "Spontaneous rosette formation with heavily haptenated sheep erythrocytes: a marker for mouse non-T lymphocytes. Most non-T lymphocytes in nonimmunized mice spontaneously bind heavily haptenated (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP)-sheep erythrocytes. Few if any T lymphocytes are rosette formers with such erythrocytes. The NNP rosette formation is suggested to be a marker for mouse non-T cells."}
{"id": "PMID:1082634", "title": "Elution and characterization of lymphocytes from rheumatoid inflammatory tissue.", "content": "Lymphocytes were eluted from the synovial tissue of 19 patients with classical rheumatoid arthritis. The tissue was minced and dissociated by treatment with crude collagenase and DNase. The cell suspension obtained was filtered and incubated in plastic culture flasks overnight at 37 degrees C. The cells that did not adhere to the plastic surface were harvested and the lymphocytes further purified by the Ficoll-Isopaque gradient centrifugation technique. The lymphocyte yield varied from 0.64 to 32 times 10(6) cells. Differential counts showed on the average 85% lymphocytes, 12% mocrophage-like cells, and variable proportions of polymorphonuclear granulocytes, unclassified cells, and dead cells. An average of 77% of the cells were viable as assessed by the trypan blue exclusion test. This cell suspension was investigated for lymphocyte populations. T lymphocytes were predominant in all experiments (mean, 73.6%). The mean percentage of B lymphocytes was 9.7%, whereas the proportion of Fc-receptor-bearing lymphocytes was on the average 6.0%.", "contents": "Elution and characterization of lymphocytes from rheumatoid inflammatory tissue. Lymphocytes were eluted from the synovial tissue of 19 patients with classical rheumatoid arthritis. The tissue was minced and dissociated by treatment with crude collagenase and DNase. The cell suspension obtained was filtered and incubated in plastic culture flasks overnight at 37 degrees C. The cells that did not adhere to the plastic surface were harvested and the lymphocytes further purified by the Ficoll-Isopaque gradient centrifugation technique. The lymphocyte yield varied from 0.64 to 32 times 10(6) cells. Differential counts showed on the average 85% lymphocytes, 12% mocrophage-like cells, and variable proportions of polymorphonuclear granulocytes, unclassified cells, and dead cells. An average of 77% of the cells were viable as assessed by the trypan blue exclusion test. This cell suspension was investigated for lymphocyte populations. T lymphocytes were predominant in all experiments (mean, 73.6%). The mean percentage of B lymphocytes was 9.7%, whereas the proportion of Fc-receptor-bearing lymphocytes was on the average 6.0%."}
{"id": "PMID:1082635", "title": "Lymphocyte subpopulations in the cerebrospinal fluid and peripheral blood in patients with multiple sclerosis.", "content": "The cerebrospinal fluid (CSF) and blood of patients with multiple sclerosis (MS) were studied with respect to the frequency of lymphocytes with intracellular immunoblobulins of different Ig classes as well as the relative frequency of B and T lymphocytes. An increased number of Ig-postive cells were found in CSF (mean, 0.52%) when compared with blood (mean 0.18%). In CSF there was a striking dominance of IgG-postive cells, very few IgA-postive cells, and almost no IgM-postive cells. The distribution in blood was approximately normal. The ratios between chi- and lambda-postive cells in CSF were all outside the range in blood. In CSF there were fewer B cells (mean, 4.7%) and more T cells (mean 74.2%) when compared with blood (mean, 11.5%) and 61.8%, respectively). The values for MS blood were approximately the same as for normal controls. The increased number of IgG-containing cells in the CSF are in agreement with earlier studies, which showed a local immunoglobulin synthesis. The increased proportion of T lymphocytes in CSF of MS patients may indicate that these cells play a role in the pathogenesis of MS.", "contents": "Lymphocyte subpopulations in the cerebrospinal fluid and peripheral blood in patients with multiple sclerosis. The cerebrospinal fluid (CSF) and blood of patients with multiple sclerosis (MS) were studied with respect to the frequency of lymphocytes with intracellular immunoblobulins of different Ig classes as well as the relative frequency of B and T lymphocytes. An increased number of Ig-postive cells were found in CSF (mean, 0.52%) when compared with blood (mean 0.18%). In CSF there was a striking dominance of IgG-postive cells, very few IgA-postive cells, and almost no IgM-postive cells. The distribution in blood was approximately normal. The ratios between chi- and lambda-postive cells in CSF were all outside the range in blood. In CSF there were fewer B cells (mean, 4.7%) and more T cells (mean 74.2%) when compared with blood (mean, 11.5%) and 61.8%, respectively). The values for MS blood were approximately the same as for normal controls. The increased number of IgG-containing cells in the CSF are in agreement with earlier studies, which showed a local immunoglobulin synthesis. The increased proportion of T lymphocytes in CSF of MS patients may indicate that these cells play a role in the pathogenesis of MS."}
{"id": "PMID:1082636", "title": "Continuous growth of mitogen-reactive B lymphocytes.", "content": "B-cell mitogens induce continous growth of murine lymphocytes in suspension cultures. Continuous growth is observed only at low cell densitiess, below 10(6) cells/ml, in regularly renewed medium containing beta-mercaptoethanol and a growth-supporting fetal calf serum. Continuous growth of  B lymphocytes in culture appears to be favored by conditions under which maturation of Ig-secreting plaque-forming cells does not occur. On the other hand, maturation is observed whenever stimulated B cells cease to divide. This maturation leads to IgM secretion followed by secretion of IgG- or IgD-like molecules. Neither continuous growth of B cells nor the 'switch' to secretion of IgG- or IgD-like molecules requires the presence of T cells.", "contents": "Continuous growth of mitogen-reactive B lymphocytes. B-cell mitogens induce continous growth of murine lymphocytes in suspension cultures. Continuous growth is observed only at low cell densitiess, below 10(6) cells/ml, in regularly renewed medium containing beta-mercaptoethanol and a growth-supporting fetal calf serum. Continuous growth of  B lymphocytes in culture appears to be favored by conditions under which maturation of Ig-secreting plaque-forming cells does not occur. On the other hand, maturation is observed whenever stimulated B cells cease to divide. This maturation leads to IgM secretion followed by secretion of IgG- or IgD-like molecules. Neither continuous growth of B cells nor the 'switch' to secretion of IgG- or IgD-like molecules requires the presence of T cells."}
{"id": "PMID:1082637", "title": "[Angiography in acute gactrointestinal hemorrhage].", "content": "The indications for and diagnostic value of visceral angiography in 30 cases of acute gastrointestinal bleeding are discussed. In one third of these emergency angiograms the etiology of the bleeding could not be demonstrated. In two thirds of the cases the source of bleeding was successfully localized. In half the positive angiograms the bleeding site was shown directly in the form of extravasation of contrast, while in the remaining half pathological findings were demonstrated without evidence of contrast media extravasation.", "contents": "[Angiography in acute gactrointestinal hemorrhage]. The indications for and diagnostic value of visceral angiography in 30 cases of acute gastrointestinal bleeding are discussed. In one third of these emergency angiograms the etiology of the bleeding could not be demonstrated. In two thirds of the cases the source of bleeding was successfully localized. In half the positive angiograms the bleeding site was shown directly in the form of extravasation of contrast, while in the remaining half pathological findings were demonstrated without evidence of contrast media extravasation."}
{"id": "PMID:1082638", "title": "[Indications and contraindications for protease inhibitors].", "content": "Clinical interest in protease inhibitors was maximal at a time when it was believed that acute bleeding tendency in some situations was due to \"primary hyperfibrinolysis\". Progress in diagnostic procedures resulted in better knowledg of the true nature of such dramatic hemorrhagic diatheses, which are due in fact to disseminated intravascular clotting with \"consumption coagulopathy\", a situation in which proteolytic inhibitors are contraindicated. Other indications have been thoroughly investigated by controlled trials, e.g. essential hypermenorrhea and postoperative hematuria after prostatectomy. Likewise, a significant protective effect of aprotinin in acute pancreatitis has been recently demonstrated. Finally, congenital deficiencies of natural inhibitors such as alpha1-antitrypsin and C1-esterase-inhibitor may benefit from substitutive therapy with exogenous or synthetic inhibitors.", "contents": "[Indications and contraindications for protease inhibitors]. Clinical interest in protease inhibitors was maximal at a time when it was believed that acute bleeding tendency in some situations was due to \"primary hyperfibrinolysis\". Progress in diagnostic procedures resulted in better knowledg of the true nature of such dramatic hemorrhagic diatheses, which are due in fact to disseminated intravascular clotting with \"consumption coagulopathy\", a situation in which proteolytic inhibitors are contraindicated. Other indications have been thoroughly investigated by controlled trials, e.g. essential hypermenorrhea and postoperative hematuria after prostatectomy. Likewise, a significant protective effect of aprotinin in acute pancreatitis has been recently demonstrated. Finally, congenital deficiencies of natural inhibitors such as alpha1-antitrypsin and C1-esterase-inhibitor may benefit from substitutive therapy with exogenous or synthetic inhibitors."}
{"id": "PMID:1082639", "title": "[Kinetics of neutrophil granulocytes].", "content": "The kinetics of neutrophilic granulocytes are briefly reviewed. The cell traverses 3 compartments during its life cycle. After a phase of proliferation and maturation in the bone marrow for a period of about 10 days, it enters the blood for an average period of 10-12 h and then migrates into tissues where it survives for an unknown length of time. Although a quantitative understanding of normal neutrophil kinetics has emerged, our knowledge of disorders of neutropoiesis is limited. Progress in the diagnosis and treatment of disorders of neutropoiesis is anticipated with improved methods of assessing quantitatively abnormal production and its regulation.", "contents": "[Kinetics of neutrophil granulocytes]. The kinetics of neutrophilic granulocytes are briefly reviewed. The cell traverses 3 compartments during its life cycle. After a phase of proliferation and maturation in the bone marrow for a period of about 10 days, it enters the blood for an average period of 10-12 h and then migrates into tissues where it survives for an unknown length of time. Although a quantitative understanding of normal neutrophil kinetics has emerged, our knowledge of disorders of neutropoiesis is limited. Progress in the diagnosis and treatment of disorders of neutropoiesis is anticipated with improved methods of assessing quantitatively abnormal production and its regulation."}
{"id": "PMID:1082640", "title": "[Proceedings: Clinical findings and coronary morphology before and after aorto-coronary venous bypass].", "content": "37 patients (mean age 51+/-8 years) were clinically and angiographically investigated before and 11+/-5 months after implantation of an aortocoronary vein graft. The rate of bypass patency was 78%. Postoperatively 57% of the patients were free of symptoms, 35% were improved and 8% unimproved or worse. The reduced incidence of angina pectoris during exercise testing appears to be correlated with graft patency, an improved vascularization index and probably the absence of preoperative myocardial infarction. The systolic time intervals and the lung-ear time at rest are of no value for differentiation of improved and unimproved patients.", "contents": "[Proceedings: Clinical findings and coronary morphology before and after aorto-coronary venous bypass]. 37 patients (mean age 51+/-8 years) were clinically and angiographically investigated before and 11+/-5 months after implantation of an aortocoronary vein graft. The rate of bypass patency was 78%. Postoperatively 57% of the patients were free of symptoms, 35% were improved and 8% unimproved or worse. The reduced incidence of angina pectoris during exercise testing appears to be correlated with graft patency, an improved vascularization index and probably the absence of preoperative myocardial infarction. The systolic time intervals and the lung-ear time at rest are of no value for differentiation of improved and unimproved patients."}
{"id": "PMID:1082641", "title": "[Intestinal hemangiomatosis].", "content": "The hemangiomas of the intestinal tract are rare benign tumors. The main clinical symptom of which is bleeding. The diagnosis of hemangiomatosis may present difficulties. In all cases of unexplained intestinal bleeding the possibility of hemangiomatosis should be considered. Should conventional radiographic studies and endoscopy, including colonoscopy, fail to locate precisely the source of bleeding, further angiographic studies are definitely indicated. Angiography is the key to the diagnosis of intestinal hemangiomatosis.", "contents": "[Intestinal hemangiomatosis]. The hemangiomas of the intestinal tract are rare benign tumors. The main clinical symptom of which is bleeding. The diagnosis of hemangiomatosis may present difficulties. In all cases of unexplained intestinal bleeding the possibility of hemangiomatosis should be considered. Should conventional radiographic studies and endoscopy, including colonoscopy, fail to locate precisely the source of bleeding, further angiographic studies are definitely indicated. Angiography is the key to the diagnosis of intestinal hemangiomatosis."}
{"id": "PMID:1082642", "title": "[Antibodies against various nuclear antigens (especially DNA and RNA proteins) in disseminated lupus erythematosus with and without kidney involvement and in other collagen diseases. Preliminary report].", "content": "The incidence of autoantibodies to various nuclear antigens was studied in 64 patients with SLE, 137 patients with various connective tissue diseases, 63 other patients and 90 controls. A positive correlation was found between SLE, nephritis, and the presence of antibody against native DNA. Antibody to denatured DNA showed no specificity for SLE and was correlated with the absence of nephritis. Antibody to RNA-protein occurred mainly in SLE, regardless of the presence or absence of nephritis.", "contents": "[Antibodies against various nuclear antigens (especially DNA and RNA proteins) in disseminated lupus erythematosus with and without kidney involvement and in other collagen diseases. Preliminary report]. The incidence of autoantibodies to various nuclear antigens was studied in 64 patients with SLE, 137 patients with various connective tissue diseases, 63 other patients and 90 controls. A positive correlation was found between SLE, nephritis, and the presence of antibody against native DNA. Antibody to denatured DNA showed no specificity for SLE and was correlated with the absence of nephritis. Antibody to RNA-protein occurred mainly in SLE, regardless of the presence or absence of nephritis."}
{"id": "PMID:1082643", "title": "[The Sharp syndrome (\"mixed connective tissue disease\")].", "content": "A new and distinct rheumatic disease with features of systemic lupus erythematosus (SLE), progressive systemic sclerosis and polymyositis is described. Typical symptons are Raynaud's syndrome, swollen hands and fingers, polyarthritis or polyarthralgia and myositis. Other symptoms are less common and include skin lesions, decreased pulmonary diffusing capacity, diminished esophageal motility, lymphadenopathy, and polyserositis. The diagnosis of mixed connective tissue disease (MCTD) can be established by demonstration of a high titer of antinuclear antibodies and antibodies against extractable nuclear antigen (anti-ENA). Both antibodies are directed against ribonuclease-sensitive antigen substrate, which permits differentiation of patients with MCTD from those with other rheumatic diseases. A relatively favourable prognosis and a good response to corticoid medication are further characteristics of this disease. Three personally observed patients with MCTD are described.", "contents": "[The Sharp syndrome (\"mixed connective tissue disease\")]. A new and distinct rheumatic disease with features of systemic lupus erythematosus (SLE), progressive systemic sclerosis and polymyositis is described. Typical symptons are Raynaud's syndrome, swollen hands and fingers, polyarthritis or polyarthralgia and myositis. Other symptoms are less common and include skin lesions, decreased pulmonary diffusing capacity, diminished esophageal motility, lymphadenopathy, and polyserositis. The diagnosis of mixed connective tissue disease (MCTD) can be established by demonstration of a high titer of antinuclear antibodies and antibodies against extractable nuclear antigen (anti-ENA). Both antibodies are directed against ribonuclease-sensitive antigen substrate, which permits differentiation of patients with MCTD from those with other rheumatic diseases. A relatively favourable prognosis and a good response to corticoid medication are further characteristics of this disease. Three personally observed patients with MCTD are described."}
{"id": "PMID:1082644", "title": "A line, not a space, represents visual distinctness of borders formed by different colors.", "content": "When observers are asked to rate the visual distinctness of borders formed by the junction of two photic stimuli, normal trichromatic subjects behave in a manner similar to that of tritanopes in a color mixture experiment. All stimuli that look the same to the tritanope produce the same border distinctness with any other stimulus. Sets of such stimuli, whose members do not form borders with each other, map as single points along a curved line, where the Euclidean distance between pairs of points representing the two stimuli is nearly proportional to the rated distinctness of the border formed between them. In the absence of luminance differences, the perception of contour apparently depends on the stimulation of only two cone types.", "contents": "A line, not a space, represents visual distinctness of borders formed by different colors. When observers are asked to rate the visual distinctness of borders formed by the junction of two photic stimuli, normal trichromatic subjects behave in a manner similar to that of tritanopes in a color mixture experiment. All stimuli that look the same to the tritanope produce the same border distinctness with any other stimulus. Sets of such stimuli, whose members do not form borders with each other, map as single points along a curved line, where the Euclidean distance between pairs of points representing the two stimuli is nearly proportional to the rated distinctness of the border formed between them. In the absence of luminance differences, the perception of contour apparently depends on the stimulation of only two cone types."}
{"id": "PMID:1082649", "title": "Urgent fibre-optic panendoscopy in upper gastro-intestinal haemorrhage.", "content": "Urgent fibre-optic panendoscopy was performed in 400 patients within 24 hours of their admission to hospital for upper gastro-intestinal haemorrhage. The cause of bleeding was established by endoscopy in 87%. No cause was found in 10% and endoscopy failed in 3%. Twenty-five per cent of the patients had an additional lesion which was not the cause of bleeding. Complications owing to endoscopy occurred in 2% of the patients. Only 27% of the patients still had blood present in the stomach at the time of endoscopy. Chronic gastric ulcer was causative in 22%, and duodenal ulcer in 20% of the patients. Haemorrhagic gastritis accounted for 13%, Mallory-Weiss tear for 8%, acute ulceration for 5% and varices for 4% of the patients. Only 60% of the patients with dyspepsia were bleeding from peptic ulcers and only 64% of the patients bleeding from ulcers had a history of dyspepsia. Follow-up studies revealed that endoscopy, in experienced hands, is an accurate investigation which allows for an improved approach to management, and which probably also reduces the mortality rate.", "contents": "Urgent fibre-optic panendoscopy in upper gastro-intestinal haemorrhage. Urgent fibre-optic panendoscopy was performed in 400 patients within 24 hours of their admission to hospital for upper gastro-intestinal haemorrhage. The cause of bleeding was established by endoscopy in 87%. No cause was found in 10% and endoscopy failed in 3%. Twenty-five per cent of the patients had an additional lesion which was not the cause of bleeding. Complications owing to endoscopy occurred in 2% of the patients. Only 27% of the patients still had blood present in the stomach at the time of endoscopy. Chronic gastric ulcer was causative in 22%, and duodenal ulcer in 20% of the patients. Haemorrhagic gastritis accounted for 13%, Mallory-Weiss tear for 8%, acute ulceration for 5% and varices for 4% of the patients. Only 60% of the patients with dyspepsia were bleeding from peptic ulcers and only 64% of the patients bleeding from ulcers had a history of dyspepsia. Follow-up studies revealed that endoscopy, in experienced hands, is an accurate investigation which allows for an improved approach to management, and which probably also reduces the mortality rate."}
{"id": "PMID:1082659", "title": "[Cryosurgery of lung metastases (author's transl)].", "content": "Cryosurgery achieves a local tumor destruction and at least in many experimental models it also produces an antitumor immunity. Cryosurgery was therefore applied to 15 patients with multiple lung metastases. In 2 cases a good palliation with a temporary standstill of tumor growth and in 3 cases of positive effect with a decrease and disappearance of metastases was noted. In two of these cases metastases of the contralateral lung also decreased or varnished. This can be explained by an immune mechanism. Big tumors and fibrosarcomas are not suited for cryosurgery, whereas hypernephroid renal carcinomas and malignant teratomas give more promising results.", "contents": "[Cryosurgery of lung metastases (author's transl)]. Cryosurgery achieves a local tumor destruction and at least in many experimental models it also produces an antitumor immunity. Cryosurgery was therefore applied to 15 patients with multiple lung metastases. In 2 cases a good palliation with a temporary standstill of tumor growth and in 3 cases of positive effect with a decrease and disappearance of metastases was noted. In two of these cases metastases of the contralateral lung also decreased or varnished. This can be explained by an immune mechanism. Big tumors and fibrosarcomas are not suited for cryosurgery, whereas hypernephroid renal carcinomas and malignant teratomas give more promising results."}
{"id": "PMID:1082660", "title": "[Clinical observations and angiographic findings before and after aortocoronary benous bypass grafting (author's transl)].", "content": "37 patients (mean age 51 +/- 8 years) were investigated clinically, mechanocardiographically and angiocardiographically before and 336 +/- 166 days after implantation of an aortocoronary vein graft. The bypass patency rate was 78%. Postoperatively 57% of the patients were free of symptoms, 35% were improved and 8% unimproved or worse. The postoperative incidence of angina pectoris during exercise testing was significantly lower in the groups with an improved vascularization-index, open vein grafts and a lack of preoperative myocardial infarction. For separating the patients only the vascularization-index and the bypass patency seem to be of value.", "contents": "[Clinical observations and angiographic findings before and after aortocoronary benous bypass grafting (author's transl)]. 37 patients (mean age 51 +/- 8 years) were investigated clinically, mechanocardiographically and angiocardiographically before and 336 +/- 166 days after implantation of an aortocoronary vein graft. The bypass patency rate was 78%. Postoperatively 57% of the patients were free of symptoms, 35% were improved and 8% unimproved or worse. The postoperative incidence of angina pectoris during exercise testing was significantly lower in the groups with an improved vascularization-index, open vein grafts and a lack of preoperative myocardial infarction. For separating the patients only the vascularization-index and the bypass patency seem to be of value."}
{"id": "PMID:1082661", "title": "[Mitral valve surgery: 10 years follow-up of 501 patients (author's transl)].", "content": "The results of mitral surgery between 1961 and 1971 are presented. Pure or predominant mitral stenosis outnumbered combined mitral valve disease and mitral incompetence by two to one. Surgical treatment comprised 291 closed mitral commissurotomies, 145 open reconstructions and 81 replacements of the mitral valve. One month mortality was 2,4% for commissurotomy, 4,8% for reconstruction, and 25% for replacement. Early mortality of the latter operation decreased to 7% since 1971. For patients with no more than slight preoperative symptoms life expectany remained unaffected for 10 years after closed commissurotomy. For patients with significant preoperative limitation or symptoms at rest 5 and 10 years life expectancy after successful surgery was 93 and 79% resp. for commissurotomy, 94 and 73% for reconstruction and 79 and 53% resp. for mitral valve replacement. In spite of a higher rate of reoperations and late deterioration of valve function the reparative operations deserve preference to the replacement of the mitral valve whenever feasible.", "contents": "[Mitral valve surgery: 10 years follow-up of 501 patients (author's transl)]. The results of mitral surgery between 1961 and 1971 are presented. Pure or predominant mitral stenosis outnumbered combined mitral valve disease and mitral incompetence by two to one. Surgical treatment comprised 291 closed mitral commissurotomies, 145 open reconstructions and 81 replacements of the mitral valve. One month mortality was 2,4% for commissurotomy, 4,8% for reconstruction, and 25% for replacement. Early mortality of the latter operation decreased to 7% since 1971. For patients with no more than slight preoperative symptoms life expectany remained unaffected for 10 years after closed commissurotomy. For patients with significant preoperative limitation or symptoms at rest 5 and 10 years life expectancy after successful surgery was 93 and 79% resp. for commissurotomy, 94 and 73% for reconstruction and 79 and 53% resp. for mitral valve replacement. In spite of a higher rate of reoperations and late deterioration of valve function the reparative operations deserve preference to the replacement of the mitral valve whenever feasible."}
{"id": "PMID:1082662", "title": "[Comparing studies on the influence of dopamine and orciprenaline on cardiac and renal function of patients after cardiac surgery (author's transl)].", "content": "Cardiac and circulatory function (cardiac output, stroke volume, heart rate, mean arterial pressure = MAP, total peripheral resistance = TPR), further renal function (PAH- and inulin clearance, filtration fraction, urinary excretion, renal sodium- and potassium excretion) were measured on 15 patients undergoing cardiac surgery to whom Dopamine and Orciprenaline were administered in increasing doses of 100 mug - up to 500 mug/min (Dopamine) and 10 mug - to 20 mug/min (Orciprenaline). An infusion of Dopamine up to 250 mug/min caused a dosis-related increase of the cardiac output up to 31% (2P less than 0.001) without essential increasing of the MAP and of the heart rate. Dopamine caused a decrease of the TPR up to 24%. Doses of Dopamine over 250 mug/min cause an increase of the MAP and of the heart rate without a real increase of the cardiac output. Renal function improved under increasing doses of Dopamine, effective renal plasma flow (ERPF) up to 74%, urinary excretion up to 130%, sodium and potassium excretion up to 60% respectively. After administering Orciprenaline in a dosis of 20 mug/min cardiac output increases up to 28%, MAP and heart rate up to 12% and 17% respectively. After the administration of Orciprenaline (20 mug/min) and Dopamine (500 mug/min) frequent extra systoles were observed without any increase of the cardiac output; MAP increased by 12%, TPR decreased by 16% after 20 mug/min of Orciprenaline. ERPF decreased slightly after Orciprenaline. Urinary excretion was reduced by a half.", "contents": "[Comparing studies on the influence of dopamine and orciprenaline on cardiac and renal function of patients after cardiac surgery (author's transl)]. Cardiac and circulatory function (cardiac output, stroke volume, heart rate, mean arterial pressure = MAP, total peripheral resistance = TPR), further renal function (PAH- and inulin clearance, filtration fraction, urinary excretion, renal sodium- and potassium excretion) were measured on 15 patients undergoing cardiac surgery to whom Dopamine and Orciprenaline were administered in increasing doses of 100 mug - up to 500 mug/min (Dopamine) and 10 mug - to 20 mug/min (Orciprenaline). An infusion of Dopamine up to 250 mug/min caused a dosis-related increase of the cardiac output up to 31% (2P less than 0.001) without essential increasing of the MAP and of the heart rate. Dopamine caused a decrease of the TPR up to 24%. Doses of Dopamine over 250 mug/min cause an increase of the MAP and of the heart rate without a real increase of the cardiac output. Renal function improved under increasing doses of Dopamine, effective renal plasma flow (ERPF) up to 74%, urinary excretion up to 130%, sodium and potassium excretion up to 60% respectively. After administering Orciprenaline in a dosis of 20 mug/min cardiac output increases up to 28%, MAP and heart rate up to 12% and 17% respectively. After the administration of Orciprenaline (20 mug/min) and Dopamine (500 mug/min) frequent extra systoles were observed without any increase of the cardiac output; MAP increased by 12%, TPR decreased by 16% after 20 mug/min of Orciprenaline. ERPF decreased slightly after Orciprenaline. Urinary excretion was reduced by a half."}
{"id": "PMID:1082663", "title": "[Primary hemangiopericytoma of the lung (author's transl)].", "content": "Primary hemangiopericytoma of the lung is exceedingly rare. The following characteristics were found in a survey of the 34 cases published so far in the world literature and in one case of our own, a 29 year old man with this mesenchymal tumor. It does not show specific clinical signs. In radiographs an isolated round unidentifiable focus is seen. The differential diagnosis has to rely on the histology. About one half of these tumors are discovered by accident. It appears that it occurs at a somewhat younger age in men than in women. Both sexes are equally represented. The treatments of choice are either lobectomy or pneumonectomy. Patients with small tumors without clinical signs appear to have a better prognosis. When recurrence or metastases develop they will be noticed within a few months. Intensive follow-up of patients operated on is essential, since histology cannot provide safe criteria on which one could decide whether the tumor was benign or malignant.", "contents": "[Primary hemangiopericytoma of the lung (author's transl)]. Primary hemangiopericytoma of the lung is exceedingly rare. The following characteristics were found in a survey of the 34 cases published so far in the world literature and in one case of our own, a 29 year old man with this mesenchymal tumor. It does not show specific clinical signs. In radiographs an isolated round unidentifiable focus is seen. The differential diagnosis has to rely on the histology. About one half of these tumors are discovered by accident. It appears that it occurs at a somewhat younger age in men than in women. Both sexes are equally represented. The treatments of choice are either lobectomy or pneumonectomy. Patients with small tumors without clinical signs appear to have a better prognosis. When recurrence or metastases develop they will be noticed within a few months. Intensive follow-up of patients operated on is essential, since histology cannot provide safe criteria on which one could decide whether the tumor was benign or malignant."}
{"id": "PMID:1082664", "title": "[The intracardial ECG during permanent pacemaker-implantation (author's transl)].", "content": "As our researches and prevailing procedures have proved a permanent pervenous pacer implantation necessitates an additional intra-cardial ECG in parallel with the practised approach under fluoroscopy and by measuring the threshold current. The patho-physiological prerequisites are explained in detail. By means of the characteristic shape of the ST-elevation and the determination of the amplitude in mV, deciding factors for an advantageous position of the electrode may be gained. In order to ensure safe function of the demand mechanism monitoring by an intra-cardial ECG to establish a sufficient signal amplitude in mV is indispensable. This is particularly applicable in case of leads with a small surface area resulting in a low threshold current value and a low amplitude of QRS-signals.", "contents": "[The intracardial ECG during permanent pacemaker-implantation (author's transl)]. As our researches and prevailing procedures have proved a permanent pervenous pacer implantation necessitates an additional intra-cardial ECG in parallel with the practised approach under fluoroscopy and by measuring the threshold current. The patho-physiological prerequisites are explained in detail. By means of the characteristic shape of the ST-elevation and the determination of the amplitude in mV, deciding factors for an advantageous position of the electrode may be gained. In order to ensure safe function of the demand mechanism monitoring by an intra-cardial ECG to establish a sufficient signal amplitude in mV is indispensable. This is particularly applicable in case of leads with a small surface area resulting in a low threshold current value and a low amplitude of QRS-signals."}
{"id": "PMID:1082665", "title": "[Isolated rupture of the interventricular septum after blunt chest trauma (author's transl)].", "content": "The mechanism, symptoms and treatment of traumatic ventricular septal defect are discussed on the basis of an own observation. To the best of our knowledge this is the first report of a rupture of the membranous septum, involving the portion between the left ventricle and the right atrium. An attempt is made to explain the mechanism of isolated traumatic ventricular septal rupture on the basis of embryological considerations. The patient died on the 11th postoperative circulatory status and inspite rather prompt diagnosis and operative treatment.", "contents": "[Isolated rupture of the interventricular septum after blunt chest trauma (author's transl)]. The mechanism, symptoms and treatment of traumatic ventricular septal defect are discussed on the basis of an own observation. To the best of our knowledge this is the first report of a rupture of the membranous septum, involving the portion between the left ventricle and the right atrium. An attempt is made to explain the mechanism of isolated traumatic ventricular septal rupture on the basis of embryological considerations. The patient died on the 11th postoperative circulatory status and inspite rather prompt diagnosis and operative treatment."}
{"id": "PMID:1082666", "title": "[Nonocclusive mesenteric vascular disease following atrio-ventricular block and pacemaker treatment. Gangrene of small and large bowel with occlusion of inferieor mesenteric artery (author's transl)].", "content": "A case of nonocclusive mesenteric vascular disease consequential to a low cardiac output syndrome in a patient with a temporary pacemaker is reported. Preexisting occlusion of the inferior mesenteric artery resulted in a total gangrene of small and large bowel. Experimental findings suggest that this occlusion could have resulted in a compensatory increase of blood flow in the superior mesenteric artery. The possibility is discussed that the preexisting necessary increase of superior mesenteric blood flow could be followed by equally increased sensitivity of regional organic systems in non-occlusive mesenteric vascular disease.", "contents": "[Nonocclusive mesenteric vascular disease following atrio-ventricular block and pacemaker treatment. Gangrene of small and large bowel with occlusion of inferieor mesenteric artery (author's transl)]. A case of nonocclusive mesenteric vascular disease consequential to a low cardiac output syndrome in a patient with a temporary pacemaker is reported. Preexisting occlusion of the inferior mesenteric artery resulted in a total gangrene of small and large bowel. Experimental findings suggest that this occlusion could have resulted in a compensatory increase of blood flow in the superior mesenteric artery. The possibility is discussed that the preexisting necessary increase of superior mesenteric blood flow could be followed by equally increased sensitivity of regional organic systems in non-occlusive mesenteric vascular disease."}
{"id": "PMID:1082667", "title": "Cold lymphocytotoxin reactivity with lymphocytes from donors in various physiological conditions.", "content": "Sera containing cold lymphocytotoxins were studied in the presence of lymphocytes from neonates, pregnant women, blood donors and old people and the degree of positivity varied according to the lymphocyte population investigated: a strongly positive response to lymphocytes from neonates and pregnant women, a weaker response to lymphocytes from old people and a very weak response to chronic leukemic lymphocytes. Cold lymphocytotoxins seem to be a supplementary method of demonstrating a difference in lymphocytic behavior.", "contents": "Cold lymphocytotoxin reactivity with lymphocytes from donors in various physiological conditions. Sera containing cold lymphocytotoxins were studied in the presence of lymphocytes from neonates, pregnant women, blood donors and old people and the degree of positivity varied according to the lymphocyte population investigated: a strongly positive response to lymphocytes from neonates and pregnant women, a weaker response to lymphocytes from old people and a very weak response to chronic leukemic lymphocytes. Cold lymphocytotoxins seem to be a supplementary method of demonstrating a difference in lymphocytic behavior."}
{"id": "PMID:1082668", "title": "Adaptation to tissue culture of the murine leukemia ASL1, a high producer of TL (thymus leukemia) antigen.", "content": "ASL1, a murine thymus derived leukemia, was adapted to tissue culture and its suitability as a source of TL antigens was examined. Tissue culture medium was developed in which the cell grew with a mean doubling time of 12.5  h and 95% viability. The quantity of antigen expressed by the cells was determined by inhibition of lysis of 51Cr labelled leukemic cells meadiated by anti-TL 1,2,3 serum. Significant quantities of the antigens were retained by the cells during exponential growth in vitro.", "contents": "Adaptation to tissue culture of the murine leukemia ASL1, a high producer of TL (thymus leukemia) antigen. ASL1, a murine thymus derived leukemia, was adapted to tissue culture and its suitability as a source of TL antigens was examined. Tissue culture medium was developed in which the cell grew with a mean doubling time of 12.5  h and 95% viability. The quantity of antigen expressed by the cells was determined by inhibition of lysis of 51Cr labelled leukemic cells meadiated by anti-TL 1,2,3 serum. Significant quantities of the antigens were retained by the cells during exponential growth in vitro."}
{"id": "PMID:1082670", "title": "Studies on the induction and expression of T cell-mediated immunity. V. Lectin-induced nonspecific cell-mediated cytotoxicity by alloimmune lymphocytes.", "content": "Murine lymphoid cells sensitized against allografts express two types of cytotoxicity measured in vitro in short-term assays of 2 to 3 hr by 51Cr release. One type of cytotoxicity is specific against target cells carrying the sensitizing alloantigens, and the other type is nonspecific against both syngeneic and unrelated target cells and requires the presence of phytohemagglutinin (PHA) or concanavalin A (ConA). Several lines of evidence are presented which demonstrate that both cytotoxic systems are mediated by the same clone of alloimmune effector T lymphocytes and that the lectin-induced cytotoxicity is not attributable to mitogenic polyclonal activation of effector cells. Studies with ConA inhibitor alpha-methyl-D-mannoside suggest a mechanism of interaction that requires the lectin to be present during the reaction. Experiments with other reagents, however, that bring about physical contact of the cells but not lysis, indicate that cytolysis can occur only through specific lectin receptors on the cells. The significance of this lectin-induced cytotoxicity is discussed.", "contents": "Studies on the induction and expression of T cell-mediated immunity. V. Lectin-induced nonspecific cell-mediated cytotoxicity by alloimmune lymphocytes. Murine lymphoid cells sensitized against allografts express two types of cytotoxicity measured in vitro in short-term assays of 2 to 3 hr by 51Cr release. One type of cytotoxicity is specific against target cells carrying the sensitizing alloantigens, and the other type is nonspecific against both syngeneic and unrelated target cells and requires the presence of phytohemagglutinin (PHA) or concanavalin A (ConA). Several lines of evidence are presented which demonstrate that both cytotoxic systems are mediated by the same clone of alloimmune effector T lymphocytes and that the lectin-induced cytotoxicity is not attributable to mitogenic polyclonal activation of effector cells. Studies with ConA inhibitor alpha-methyl-D-mannoside suggest a mechanism of interaction that requires the lectin to be present during the reaction. Experiments with other reagents, however, that bring about physical contact of the cells but not lysis, indicate that cytolysis can occur only through specific lectin receptors on the cells. The significance of this lectin-induced cytotoxicity is discussed."}
{"id": "PMID:1082669", "title": "Unexpected limitations in the use of commercial antiglobulin reagents.", "content": "The majority of antiglobulin sera used in blood banks in the USA are commercially prepared immune rabbit sera, designed to be reactive only with human red blood cells sensitized with immunoglobulins or complement components. Current manufacturing methods result in a product that gives reliable specific reactions provided that the test red blood cells are normal, particularly with respect to sialic acid levels. This report describes our findings of false positive antiglobulin tests caused by incompletely absorbed antiglobulin reagents when cells with low sialic acid levels were tested. An evaluation of nine commercially prepared antiglobulin reagents revealed, in many of them, the presence of anti-species antibody, anti-T, and anti-Tn. Blood bank personnel must be aware of these characteristics especially when testing either enzyme premodified or polyagglutinable cells. The use of incompletely absorbed reagents might account for positive direct antiglobulin tests that are encountered occasionally in apparently normal healthy individuals. Furthermore, recent protocols advocating the use of trypsinized cells for the evaluation of anti-C3d activity of antiglobulin sera are invalid if the reagent is inadequately absorbed of anti-species agglutinins.", "contents": "Unexpected limitations in the use of commercial antiglobulin reagents. The majority of antiglobulin sera used in blood banks in the USA are commercially prepared immune rabbit sera, designed to be reactive only with human red blood cells sensitized with immunoglobulins or complement components. Current manufacturing methods result in a product that gives reliable specific reactions provided that the test red blood cells are normal, particularly with respect to sialic acid levels. This report describes our findings of false positive antiglobulin tests caused by incompletely absorbed antiglobulin reagents when cells with low sialic acid levels were tested. An evaluation of nine commercially prepared antiglobulin reagents revealed, in many of them, the presence of anti-species antibody, anti-T, and anti-Tn. Blood bank personnel must be aware of these characteristics especially when testing either enzyme premodified or polyagglutinable cells. The use of incompletely absorbed reagents might account for positive direct antiglobulin tests that are encountered occasionally in apparently normal healthy individuals. Furthermore, recent protocols advocating the use of trypsinized cells for the evaluation of anti-C3d activity of antiglobulin sera are invalid if the reagent is inadequately absorbed of anti-species agglutinins."}
{"id": "PMID:1082679", "title": "The titre of IgG anti-A/B in the serum of group O mothers of incompatible infants with early neonatal jaundice.", "content": "The titre of IgG anti-A/B was determined by means of a 2-mercaptoethanol test in the serum of 80 blood group O mothers, who gave birth at term to A/B incompatible infants, who developed early neonatal jaundice with a serum bilirubin level of over 10 mg/100 ml. The titres were compared with the results of the direct antiglobulin test performed on the red cells from the cord blood and the necessity for exchange transfusion. Only in mothers of group A infants, a significant correlation was found between the titre of IgG anti-A and the occurrence of a positive antiglobulin test. In no case the need for an exchange transfusion was correlated with the IgG anti-A/B titre.", "contents": "The titre of IgG anti-A/B in the serum of group O mothers of incompatible infants with early neonatal jaundice. The titre of IgG anti-A/B was determined by means of a 2-mercaptoethanol test in the serum of 80 blood group O mothers, who gave birth at term to A/B incompatible infants, who developed early neonatal jaundice with a serum bilirubin level of over 10 mg/100 ml. The titres were compared with the results of the direct antiglobulin test performed on the red cells from the cord blood and the necessity for exchange transfusion. Only in mothers of group A infants, a significant correlation was found between the titre of IgG anti-A and the occurrence of a positive antiglobulin test. In no case the need for an exchange transfusion was correlated with the IgG anti-A/B titre."}
{"id": "PMID:1082678", "title": "[Complex compounds of heparin in obstetric hemorrhages].", "content": "The so-called uncontrollable hemorrhage in obstetric patients correlated mainly with appearance in blood of complex heparin compounds, primarily fibrinogen-heparin, adrenaline-heparin, noradrenaline-heparin and, less frequently, plasminogen-heparin. The heparin complexes were observed in circulation when the anticoagulation system was stimulated due to appearance of thrombin in the blood stream. The complexes possessed and anticoagulation and antipolymerization effects on fibrin-monomer and caused nonenzymatic lysis of unstabilized fibrin clots.", "contents": "[Complex compounds of heparin in obstetric hemorrhages]. The so-called uncontrollable hemorrhage in obstetric patients correlated mainly with appearance in blood of complex heparin compounds, primarily fibrinogen-heparin, adrenaline-heparin, noradrenaline-heparin and, less frequently, plasminogen-heparin. The heparin complexes were observed in circulation when the anticoagulation system was stimulated due to appearance of thrombin in the blood stream. The complexes possessed and anticoagulation and antipolymerization effects on fibrin-monomer and caused nonenzymatic lysis of unstabilized fibrin clots."}
{"id": "PMID:1082683", "title": "[Electrotherapy of life-threatening arrhythmia in acute myocardial infarct].", "content": "As a result of an adjustment of the experiences which were made in four clinics with the electrotherapy of life-threatening disturbances of rhythm in acute myocardial infarction recommendations for the use of this therapy are given. The value of the various methods of electrotherapy compared with medicamentous treatment are elaborated.", "contents": "[Electrotherapy of life-threatening arrhythmia in acute myocardial infarct]. As a result of an adjustment of the experiences which were made in four clinics with the electrotherapy of life-threatening disturbances of rhythm in acute myocardial infarction recommendations for the use of this therapy are given. The value of the various methods of electrotherapy compared with medicamentous treatment are elaborated."}
{"id": "PMID:1082684", "title": "[Effects of blood sampling on enzyme activities in the serum of small laboratory animals (author's transl)].", "content": "Because of the difficulties in drawing blood for clinical chemistry in small laboratory animals there exist many methods for sampling blood and the preparation of serum, none of which is generally accepted or well standardised. It was the aim of this study to investigate the effects of sampling techniques on normal values of enzyme activities in the serum of rat and mouse. The activities of the following enzymes were determined: sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, glutamate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, pyruvate kinase, creatine kinase, myokinase, alkaline phosphatase and leucine aminopeptidase. In addition plasmaproteins, urea and inorganic phosphorus were measured. In rats blood was obtained from the following sites: retroorbital venous plexus, jugular vein, heart and ventral aorta. In mice blood was sampled from the jugular vein and the ventral aorta. Shifts of water from the interstitial to the intravascular space due to hypovolemia occurring during the experimental procedure were followed up by measuring the hematocrit and the distribution of radioiodide labelled albumin. In rats the activities of lactate dehydrogenase, malate dehydrogenase, aspartate aminotransferase, pyruvate kinase, creatine kinase and myokinase found in blood serum obtained from the retroorbital venous plexus and the ventral aorta were too high compared to the other sampling sites. Activities of alkaline phosphatase and alanine aminotransferase were slightly  elevated when blood was sampled from the punctured retroorbital venous plexus. Small differences in plasmaproteins and hematocrit values were found to be due to acute shifts of water within the extracellular space. In mice the activities of lactate dehydrogenase, malate dehydrogenase, aspartate aminotransferase and myokinase were found to be too high in blood serum obtained from the ventral aorta. Efflux of enzymes from damaged cells and the interstitial space ive caused erroneous results too, but only to a minor extent. The most reliable method for blood sampling in rat and mouse is the cannulation of the jugular vein. The heart puncture can be recommended too. Attention should be paid, however, to the possibility of aspirating disrupted muscle cells through the inserted needle.", "contents": "[Effects of blood sampling on enzyme activities in the serum of small laboratory animals (author's transl)]. Because of the difficulties in drawing blood for clinical chemistry in small laboratory animals there exist many methods for sampling blood and the preparation of serum, none of which is generally accepted or well standardised. It was the aim of this study to investigate the effects of sampling techniques on normal values of enzyme activities in the serum of rat and mouse. The activities of the following enzymes were determined: sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, glutamate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, pyruvate kinase, creatine kinase, myokinase, alkaline phosphatase and leucine aminopeptidase. In addition plasmaproteins, urea and inorganic phosphorus were measured. In rats blood was obtained from the following sites: retroorbital venous plexus, jugular vein, heart and ventral aorta. In mice blood was sampled from the jugular vein and the ventral aorta. Shifts of water from the interstitial to the intravascular space due to hypovolemia occurring during the experimental procedure were followed up by measuring the hematocrit and the distribution of radioiodide labelled albumin. In rats the activities of lactate dehydrogenase, malate dehydrogenase, aspartate aminotransferase, pyruvate kinase, creatine kinase and myokinase found in blood serum obtained from the retroorbital venous plexus and the ventral aorta were too high compared to the other sampling sites. Activities of alkaline phosphatase and alanine aminotransferase were slightly  elevated when blood was sampled from the punctured retroorbital venous plexus. Small differences in plasmaproteins and hematocrit values were found to be due to acute shifts of water within the extracellular space. In mice the activities of lactate dehydrogenase, malate dehydrogenase, aspartate aminotransferase and myokinase were found to be too high in blood serum obtained from the ventral aorta. Efflux of enzymes from damaged cells and the interstitial space ive caused erroneous results too, but only to a minor extent. The most reliable method for blood sampling in rat and mouse is the cannulation of the jugular vein. The heart puncture can be recommended too. Attention should be paid, however, to the possibility of aspirating disrupted muscle cells through the inserted needle."}
{"id": "PMID:1082685", "title": "[Cellular immune reactions of the white mouse after superinfection with Toxoplasma gondii (author's transl)].", "content": "After superinfection of immunized mice with a virulent strain of Toxoplasma (BK) these mice don't die. There is formation of a cellrich exudate with only very few toxoplasmas. The number of the different exudate-cells (macrophages, big, middlesized and small lymphocytes and heterophils) turn in direction of the lymphocytes in that way, that after the 3rd day of infection 70% of all exudate-cells belong to the group of lymphocytes. They seem to cause the death of the toxoplasmas. After in vitro incubation of trophozoites and exudate-cells of immune mice for 2 hours at 37 degrees C nearly 80% of the added toxoplasmas are killed. Electronmicroscopical pictures show, that this killing is produced by a process of lysis.", "contents": "[Cellular immune reactions of the white mouse after superinfection with Toxoplasma gondii (author's transl)]. After superinfection of immunized mice with a virulent strain of Toxoplasma (BK) these mice don't die. There is formation of a cellrich exudate with only very few toxoplasmas. The number of the different exudate-cells (macrophages, big, middlesized and small lymphocytes and heterophils) turn in direction of the lymphocytes in that way, that after the 3rd day of infection 70% of all exudate-cells belong to the group of lymphocytes. They seem to cause the death of the toxoplasmas. After in vitro incubation of trophozoites and exudate-cells of immune mice for 2 hours at 37 degrees C nearly 80% of the added toxoplasmas are killed. Electronmicroscopical pictures show, that this killing is produced by a process of lysis."}
{"id": "PMID:1082689", "title": "[Therapeutic results achieved through the advantage of a combination of oral metronidazole and vaginal natamycin tablets in urogenital protozoan, trichobacterial and blastomyces infections].", "content": "Vaginal inflammations caused by Trichomonas, Leptothrix vaginalis or Candida and other less common organisms respectively, were treated very successfully with a combination of metronidazole orally and a natamycin preparation vaginally (Pimafucin vaginal tablets). In women with urogenital Trichomoniasis a cure was obtained in 96,1% of the cases (microbiologically controlled); Candida mycoses of the vagina were cured clinically in 89% of the cases after the first course of treatment and mycologically in 79% of the cases. Of special importance is the rapid and successful elimination of the mixed infection before birth, because this diminishes perinatal morbidity of the fetus. The combination treatment of bilateral, constantly interchanging Candida and Trichomonas infections of the vagina is of importance to both sexes, especially with respect to the elimination of sterility. From the onco-gynecological point of view the combination treatment with metronidazole and natamycin appeared to be of value as it limits the incidence of dyskariosis of the epithelial cells, which occur frequently in cases of inflammation of the vagina or cervix uteri. No teratogenic effect was observed with this combination when applied during pregnancy.", "contents": "[Therapeutic results achieved through the advantage of a combination of oral metronidazole and vaginal natamycin tablets in urogenital protozoan, trichobacterial and blastomyces infections]. Vaginal inflammations caused by Trichomonas, Leptothrix vaginalis or Candida and other less common organisms respectively, were treated very successfully with a combination of metronidazole orally and a natamycin preparation vaginally (Pimafucin vaginal tablets). In women with urogenital Trichomoniasis a cure was obtained in 96,1% of the cases (microbiologically controlled); Candida mycoses of the vagina were cured clinically in 89% of the cases after the first course of treatment and mycologically in 79% of the cases. Of special importance is the rapid and successful elimination of the mixed infection before birth, because this diminishes perinatal morbidity of the fetus. The combination treatment of bilateral, constantly interchanging Candida and Trichomonas infections of the vagina is of importance to both sexes, especially with respect to the elimination of sterility. From the onco-gynecological point of view the combination treatment with metronidazole and natamycin appeared to be of value as it limits the incidence of dyskariosis of the epithelial cells, which occur frequently in cases of inflammation of the vagina or cervix uteri. No teratogenic effect was observed with this combination when applied during pregnancy."}
{"id": "PMID:1082691", "title": "[Quantitative determination of potassium content of spinal motor neurons frogs by x-ray microanalysis].", "content": "X-ray microanalysis has been made of the potassium content of motoneurons in the frogs Rana ridibunda and R. temporaria. The tissue was frozen in isopentane up to-160 degrees, dried in vacuum at-50 degrees, embedded into epoxy resin and sectioned by ultramicrotome. The obtained mean value of intracellular potassium concentration-65 mM/kg of wet weight or 80 mM/l of intracellular water-is in a good agreement with the results of calculation of intracellular potassium from equilibrium potential of post-spike after-hyperpolarization.", "contents": "[Quantitative determination of potassium content of spinal motor neurons frogs by x-ray microanalysis]. X-ray microanalysis has been made of the potassium content of motoneurons in the frogs Rana ridibunda and R. temporaria. The tissue was frozen in isopentane up to-160 degrees, dried in vacuum at-50 degrees, embedded into epoxy resin and sectioned by ultramicrotome. The obtained mean value of intracellular potassium concentration-65 mM/kg of wet weight or 80 mM/l of intracellular water-is in a good agreement with the results of calculation of intracellular potassium from equilibrium potential of post-spike after-hyperpolarization."}
{"id": "PMID:1082690", "title": "[Seasonal changes in cutaneous blood vessel tone in the frog Rana esculenta].", "content": "Experiments have been carried out on frogs immobilized by diplacine. The diameter of arteries and veins in the web was measured before and after sectioning the sciatic nerve. Denervation of the hindlimb resulted in dilatation of web vessels, which was different at various seasons. Changes in the arterial tone were found to be polyphasic. The highest tone was observed in March, April and September, the lowest one-in May, August, October and January. Changes in the venous tone are less pronounced and follow biphasic pattern, the tone being increased in spring and summer and decreased in autumn and winter. Stimulation of the peripheral end of the sectioned nerve leads to the constriction of web vessels, its amplitude being dependent on the level of vascular tone at the given period of year. It is suggested that seasonal changes in the tone of cutaneous arteries are partially based on changes in the reactivity of the blood vessels.", "contents": "[Seasonal changes in cutaneous blood vessel tone in the frog Rana esculenta]. Experiments have been carried out on frogs immobilized by diplacine. The diameter of arteries and veins in the web was measured before and after sectioning the sciatic nerve. Denervation of the hindlimb resulted in dilatation of web vessels, which was different at various seasons. Changes in the arterial tone were found to be polyphasic. The highest tone was observed in March, April and September, the lowest one-in May, August, October and January. Changes in the venous tone are less pronounced and follow biphasic pattern, the tone being increased in spring and summer and decreased in autumn and winter. Stimulation of the peripheral end of the sectioned nerve leads to the constriction of web vessels, its amplitude being dependent on the level of vascular tone at the given period of year. It is suggested that seasonal changes in the tone of cutaneous arteries are partially based on changes in the reactivity of the blood vessels."}
{"id": "PMID:1082695", "title": "[Natural immune response and immunological memory in the effects of a 2d unrelated antigen].", "content": "In combined administration of two nonaffiliated erythrocytic antigens there can be seen both depression and stimulation of the immunological response to the participating antigens. The end result of the interaction depended on the \"power\", the dose and the order of administration of the antigens. Distribution of the antigen in the animals whose immune response was depressed as a result of preliminary administration of the nonaffiliated antigen remained unchanged. The factor determining the effect on the immunological response to the other antigen did not serve as an antibody.", "contents": "[Natural immune response and immunological memory in the effects of a 2d unrelated antigen]. In combined administration of two nonaffiliated erythrocytic antigens there can be seen both depression and stimulation of the immunological response to the participating antigens. The end result of the interaction depended on the \"power\", the dose and the order of administration of the antigens. Distribution of the antigen in the animals whose immune response was depressed as a result of preliminary administration of the nonaffiliated antigen remained unchanged. The factor determining the effect on the immunological response to the other antigen did not serve as an antibody."}
{"id": "PMID:1082696", "title": "[Study of the mechanisms of autoallergic reactions in cirrhosis of the liver].", "content": "Cell and humoral immunological tests were used in clinical examination of 47 patients suffering from cirrhosis of the liver; a mixed character of autoallergy was revealed in this form of pathology. Reproduction of passive anaphylaxis on guinea pigs with the aid of the sera and leukocytes of these patients pointed to the prevalence of cell allergy. Passive transmission of increased sensitivity to human hepatocytes from guinea pigs, preliminarily sensitized with salt extracts from human hepatocytes, to intact animals was realized. Active sensitization was accompanied by the formation of mixed allergy to the antigen obtained from the liver of a person who died accidentally, with the prevalence of the immediate type of reaction.", "contents": "[Study of the mechanisms of autoallergic reactions in cirrhosis of the liver]. Cell and humoral immunological tests were used in clinical examination of 47 patients suffering from cirrhosis of the liver; a mixed character of autoallergy was revealed in this form of pathology. Reproduction of passive anaphylaxis on guinea pigs with the aid of the sera and leukocytes of these patients pointed to the prevalence of cell allergy. Passive transmission of increased sensitivity to human hepatocytes from guinea pigs, preliminarily sensitized with salt extracts from human hepatocytes, to intact animals was realized. Active sensitization was accompanied by the formation of mixed allergy to the antigen obtained from the liver of a person who died accidentally, with the prevalence of the immediate type of reaction."}
{"id": "PMID:1082693", "title": "[Properties of rhythmically active neurons of lymphatic heart center of the frog Rana temporaria].", "content": "During extracellular recordings of the activity of spinal neuronal units in situ, it was found that rhythmically active neurons respond to antidromic stimulation of ventral roots (X, XI), which include motor heart fibers. The amplitude of the responses of these motoneurons to continuous rhythmic antidromic stimulation exhibits slow rhythmic oscillations, reaching the lowest value to the onset of the spontaneous spike potentials. In isolated spinal cord preparation (from VIII segment), rhythmic activity of the center studied was preserved, which indicates the capacity of central structures to automatic activity.", "contents": "[Properties of rhythmically active neurons of lymphatic heart center of the frog Rana temporaria]. During extracellular recordings of the activity of spinal neuronal units in situ, it was found that rhythmically active neurons respond to antidromic stimulation of ventral roots (X, XI), which include motor heart fibers. The amplitude of the responses of these motoneurons to continuous rhythmic antidromic stimulation exhibits slow rhythmic oscillations, reaching the lowest value to the onset of the spontaneous spike potentials. In isolated spinal cord preparation (from VIII segment), rhythmic activity of the center studied was preserved, which indicates the capacity of central structures to automatic activity."}
{"id": "PMID:1082692", "title": "[Synaptic organization of the vestibular nucleus in the medulla oblongata of the frog Rana temporaria].", "content": "Studies have been made on the synaptic organization of ventral vestibular nucleus of the frog R. temporaria. Besides numerous axo-somatic and axo-dendritic synapses, 3-component axo-axonal synaptic contacts were revealed which indicate a possibility of presynaptic inhibition in the nucleus studied. Three types of membrane specializations were found in axo-somatic and axo-dendritic synaptic contacts-active zone of chemical synapse, symmetric thickening of the membranes of desmosomal type and close linking of the membranes of electrotonic synapse. Functional role of the observed contacts is discussed.", "contents": "[Synaptic organization of the vestibular nucleus in the medulla oblongata of the frog Rana temporaria]. Studies have been made on the synaptic organization of ventral vestibular nucleus of the frog R. temporaria. Besides numerous axo-somatic and axo-dendritic synapses, 3-component axo-axonal synaptic contacts were revealed which indicate a possibility of presynaptic inhibition in the nucleus studied. Three types of membrane specializations were found in axo-somatic and axo-dendritic synaptic contacts-active zone of chemical synapse, symmetric thickening of the membranes of desmosomal type and close linking of the membranes of electrotonic synapse. Functional role of the observed contacts is discussed."}
{"id": "PMID:1082697", "title": "[The palatine tonsils and immunity. Report VII. Soluble factors produced by the lymphocytes of the palatine tonsils in vitro].", "content": "A study was made of the production of a blastogenic factor and lymphotoxin in the cultures of lymphocytes of the palatine tonsils removed from patients with chronic tonsillitis; the activity of this blastogenic factor and lymphotoxin was studied in the test-cultures of autologous and allogenic lymphocytes and the transplantable HeLa cells. The antigens of the pathogenic streptococcus and staphylococcus induced production of the blastogenic factor and lymphotoxin; as to the antigens of saprophytic bacteria-they produced no such action. The antigen-specific blastogenic factor intensified the immune response to the homologous antigen, whereas the factor obtained in stimulation of lymphocytes with PHA-to all the microbial antigens under study.", "contents": "[The palatine tonsils and immunity. Report VII. Soluble factors produced by the lymphocytes of the palatine tonsils in vitro]. A study was made of the production of a blastogenic factor and lymphotoxin in the cultures of lymphocytes of the palatine tonsils removed from patients with chronic tonsillitis; the activity of this blastogenic factor and lymphotoxin was studied in the test-cultures of autologous and allogenic lymphocytes and the transplantable HeLa cells. The antigens of the pathogenic streptococcus and staphylococcus induced production of the blastogenic factor and lymphotoxin; as to the antigens of saprophytic bacteria-they produced no such action. The antigen-specific blastogenic factor intensified the immune response to the homologous antigen, whereas the factor obtained in stimulation of lymphocytes with PHA-to all the microbial antigens under study."}
{"id": "PMID:1082694", "title": "[Histochemical studies of oxidative enzymes in the gastric mucosa of sheat-fishes, frogs, tortoises and pigeons].", "content": "Oxyntic cells of gastric glands of the sheat-fish Silurus glanis, frog Rana temporaria, tortoise Agrionemis horsfieldi and pigeon Columba livia exhibit intensive reactions on dehydrogenases of lactate, alpha-ketoglutarate, glutamate, succinate, malate and glucose-6-phosphate which indicate high energy metabolism necessary for the production of HCl by these cells. Cytoplasm of these cells is stained for the presence of nucleic acids, which may be associated with the other function of the oxyntic cells, i.e. the production of the enzymes of gastric juice.", "contents": "[Histochemical studies of oxidative enzymes in the gastric mucosa of sheat-fishes, frogs, tortoises and pigeons]. Oxyntic cells of gastric glands of the sheat-fish Silurus glanis, frog Rana temporaria, tortoise Agrionemis horsfieldi and pigeon Columba livia exhibit intensive reactions on dehydrogenases of lactate, alpha-ketoglutarate, glutamate, succinate, malate and glucose-6-phosphate which indicate high energy metabolism necessary for the production of HCl by these cells. Cytoplasm of these cells is stained for the presence of nucleic acids, which may be associated with the other function of the oxyntic cells, i.e. the production of the enzymes of gastric juice."}
{"id": "PMID:1082699", "title": "Latent myocardial contractile impairment in patients with angina pectoris.", "content": "Intravenous injection of Angiotensin II gives rise to a rapid and transient increase of the afterload of the left ventricle (increased peripheral resistances). In this haemodynamic setting the functional behaviour of the left ventricle, through indirect parameters such as systolic time intervals, was compared in 14 patients with angina and in 15 normal control subjects. As regards blood pressure and heart rate a similar behaviour was registered in the two groups in basal conditions; directly after the intravenous injection of Angiotensin II changes suggesting impairment of left ventricular function failure have been obsered in patients with angina pectoris.", "contents": "Latent myocardial contractile impairment in patients with angina pectoris. Intravenous injection of Angiotensin II gives rise to a rapid and transient increase of the afterload of the left ventricle (increased peripheral resistances). In this haemodynamic setting the functional behaviour of the left ventricle, through indirect parameters such as systolic time intervals, was compared in 14 patients with angina and in 15 normal control subjects. As regards blood pressure and heart rate a similar behaviour was registered in the two groups in basal conditions; directly after the intravenous injection of Angiotensin II changes suggesting impairment of left ventricular function failure have been obsered in patients with angina pectoris."}
{"id": "PMID:1082700", "title": "A two compartment open model for digoxin pharmacokinetics in patients receiving a wide range of digoxin doses.", "content": "The pharmacokinetic parameters for a two compartment open model were defined for six patients receiving a wide range of Digoxin doses. It was demonstrated that the two compartment model is a valid one to use for Digoxin pharmacokinetics. This model is an useful concept because it can explain the necessity to vary Digoxin dosage in patients with different body weights, the time course of the effect of Digoxin and certain causes of increased tolerance to Digoxin. There were no alterations in the parameters of this model or of the percent of an injected Digoxin dose excreted in the urine and stool in our patients in atrial fibrillation who appeared to require larger doses to control their ventricular rates. This also suggests that the kinetics of excretion of Digoxin are not influenced by altering the Digoxin dose.", "contents": "A two compartment open model for digoxin pharmacokinetics in patients receiving a wide range of digoxin doses. The pharmacokinetic parameters for a two compartment open model were defined for six patients receiving a wide range of Digoxin doses. It was demonstrated that the two compartment model is a valid one to use for Digoxin pharmacokinetics. This model is an useful concept because it can explain the necessity to vary Digoxin dosage in patients with different body weights, the time course of the effect of Digoxin and certain causes of increased tolerance to Digoxin. There were no alterations in the parameters of this model or of the percent of an injected Digoxin dose excreted in the urine and stool in our patients in atrial fibrillation who appeared to require larger doses to control their ventricular rates. This also suggests that the kinetics of excretion of Digoxin are not influenced by altering the Digoxin dose."}
{"id": "PMID:1082701", "title": "Massive digoxin intoxication. Report of a case with serum digoxin level correlation.", "content": "The clinical course is described of a patient who took an overdose of 15 mg of digoxin in a suicidal attempt. He developed several cardiac arrhythmias like atrial fibrillation, atrial flutter, sino-atrial block. atrio-ventricular block 3 degree and ventricular extrasystoles. His plasma digoxin level rose to 38.7 ng/ml as determined by radioimmunoassay. He was treated with Aprindine and recovered without sequelae. A survey of the complications of acute massive digoxin intoxication is offered as well as a summary of treatment.", "contents": "Massive digoxin intoxication. Report of a case with serum digoxin level correlation. The clinical course is described of a patient who took an overdose of 15 mg of digoxin in a suicidal attempt. He developed several cardiac arrhythmias like atrial fibrillation, atrial flutter, sino-atrial block. atrio-ventricular block 3 degree and ventricular extrasystoles. His plasma digoxin level rose to 38.7 ng/ml as determined by radioimmunoassay. He was treated with Aprindine and recovered without sequelae. A survey of the complications of acute massive digoxin intoxication is offered as well as a summary of treatment."}
{"id": "PMID:1082702", "title": "Radiation-induced heart disease.", "content": "A 45-year old woman underwent a radical mastectomy in 1965 for carcinoma of the left breast with metastasis in the left axillar lymph nodes. Fifty per cent of the heart received 4,000 rads during post-operative X-ray therapy. Patient developed radiopneumonia and symptoms of acute pericarditis in 1967. Constrictive pericarditis developed gradually from 1972 on. A pericardiectomy was performed in June 1974 and a thickened pericardium could be removed. Light and electron microscopic examination of a surgical biopsy of the left ventricular epi-myocardium revealed epicardial fibrosis, interstitial fibrosis of the myocardium and perivascular fibrosis. The diagnosis of post-radiation pericarditis was made. The myocardial involvement may be responsible for the subsequent clinical course.", "contents": "Radiation-induced heart disease. A 45-year old woman underwent a radical mastectomy in 1965 for carcinoma of the left breast with metastasis in the left axillar lymph nodes. Fifty per cent of the heart received 4,000 rads during post-operative X-ray therapy. Patient developed radiopneumonia and symptoms of acute pericarditis in 1967. Constrictive pericarditis developed gradually from 1972 on. A pericardiectomy was performed in June 1974 and a thickened pericardium could be removed. Light and electron microscopic examination of a surgical biopsy of the left ventricular epi-myocardium revealed epicardial fibrosis, interstitial fibrosis of the myocardium and perivascular fibrosis. The diagnosis of post-radiation pericarditis was made. The myocardial involvement may be responsible for the subsequent clinical course."}
{"id": "PMID:1082703", "title": "The diagnosis or ventricular tachycardia with ventriculo-atrial conduction.", "content": "A 57 year old man developed recurrent tachycardia with QRS complexes of 0.13 second in duration at a rate of 140 beats per minute. Inverted P waves appeared to follow each QRS complex in the electrocardiographic leads II, III and aVF. The differentiation between ventricular tachycardia with1 : 1 V-A conduction and supraventricular tachycardia with aberrant ventricular conduction was difficult to make from the surface electrocardiogram. This differentiation is important for selection of appropriate therapy. The diagnosis of ventricular tachycardia with 1 : 1 V-A conduction was clearly established in this case on simultaneous recording of surface electrocardiogram, His bundle electrogram and high right atrial electrogram.", "contents": "The diagnosis or ventricular tachycardia with ventriculo-atrial conduction. A 57 year old man developed recurrent tachycardia with QRS complexes of 0.13 second in duration at a rate of 140 beats per minute. Inverted P waves appeared to follow each QRS complex in the electrocardiographic leads II, III and aVF. The differentiation between ventricular tachycardia with1 : 1 V-A conduction and supraventricular tachycardia with aberrant ventricular conduction was difficult to make from the surface electrocardiogram. This differentiation is important for selection of appropriate therapy. The diagnosis of ventricular tachycardia with 1 : 1 V-A conduction was clearly established in this case on simultaneous recording of surface electrocardiogram, His bundle electrogram and high right atrial electrogram."}
{"id": "PMID:1082704", "title": "Radical emergency operation for prolapsed and strangulated haemorrhoids.", "content": "Conservative treatement of prolapsed and strangulated haemorrhoids is still widely used. According to several recent publications, however, emergency operation is a reasonable, safe, and effective treatment. Twenty-five patients with prolapsed and strangulated haemorrhoids underwent operation by the Milligan method. The results are compared with those in a similiar group having elective operation of chronic haemorrhoids. Since there were no differences in postoperative complications, length of stay in hospital, period off work, or late results, and since conservative treatment entails lengthy, painful treatment in bed and a long period off work, emergency operation is recommended for all strangulated haemorrhoids.", "contents": "Radical emergency operation for prolapsed and strangulated haemorrhoids. Conservative treatement of prolapsed and strangulated haemorrhoids is still widely used. According to several recent publications, however, emergency operation is a reasonable, safe, and effective treatment. Twenty-five patients with prolapsed and strangulated haemorrhoids underwent operation by the Milligan method. The results are compared with those in a similiar group having elective operation of chronic haemorrhoids. Since there were no differences in postoperative complications, length of stay in hospital, period off work, or late results, and since conservative treatment entails lengthy, painful treatment in bed and a long period off work, emergency operation is recommended for all strangulated haemorrhoids."}
{"id": "PMID:1082705", "title": "[T and B lymphocytes in peripheral blood of splenectomized subjects].", "content": "The report is a part of more extensive studies on the role of the spleen in immune processes. Assessing the ability of lymphocytes T to non-immunological binding of sheep erythrocytes and the ability of B cells to bind immunological complexes through the receptor for C3 component of complement, and the presence of immunoglobulins on the surface of lymphocytes B the number of these cells in peripheral blood was determined in 14 healthy individuals who had been splenectomized. In all these subjects the degree of blastic transformation of lymphocytes in PHA-stimulated 3 day-old white blood cell cultures was determined. It was found that the number of T and B lymphocytes in the peripheral blood of splenectomized and non-splenectomized patients was similiar. Lymphocytes obtained from splenectomized patients had however, an impairment of transformation ability after PHA stimulation. It is suggested that, apart from determination of T and B cell pool in the peripheral blood, an evaluation of their transformation ability after PHA stimulation is necessary for assessment of the immunological state in vitro investigations.", "contents": "[T and B lymphocytes in peripheral blood of splenectomized subjects]. The report is a part of more extensive studies on the role of the spleen in immune processes. Assessing the ability of lymphocytes T to non-immunological binding of sheep erythrocytes and the ability of B cells to bind immunological complexes through the receptor for C3 component of complement, and the presence of immunoglobulins on the surface of lymphocytes B the number of these cells in peripheral blood was determined in 14 healthy individuals who had been splenectomized. In all these subjects the degree of blastic transformation of lymphocytes in PHA-stimulated 3 day-old white blood cell cultures was determined. It was found that the number of T and B lymphocytes in the peripheral blood of splenectomized and non-splenectomized patients was similiar. Lymphocytes obtained from splenectomized patients had however, an impairment of transformation ability after PHA stimulation. It is suggested that, apart from determination of T and B cell pool in the peripheral blood, an evaluation of their transformation ability after PHA stimulation is necessary for assessment of the immunological state in vitro investigations."}
{"id": "PMID:1082706", "title": "The nystagmus threshold in turning test in different age groups and in patients suffering from otosclerosis.", "content": "The threshold of nystagmus during angular acceleration and deceleration was investigated in two series of healthy persons with an average age of 20 and 42 years and in series of otosclerotic patients with an average age of 42 years. It was shown that age as such does not influence the thresholds, but a lesion in the inner ear, such as otosclerosis, causes higher thresholds even in younger subjects.", "contents": "The nystagmus threshold in turning test in different age groups and in patients suffering from otosclerosis. The threshold of nystagmus during angular acceleration and deceleration was investigated in two series of healthy persons with an average age of 20 and 42 years and in series of otosclerotic patients with an average age of 42 years. It was shown that age as such does not influence the thresholds, but a lesion in the inner ear, such as otosclerosis, causes higher thresholds even in younger subjects."}
{"id": "PMID:1082709", "title": "Immunological and histological studies of temporal arteries from patients with temporal arteritis and/or polymyalgia rheumatica.", "content": "Biopsies from the temporal arteries of 62 out of 80 patients presenting the clinical picture of temporal arteritis and/or polymyalgia rheumatica showed morphologically active or healed arteritis. Fifty-five of these biopsies revealed anti-IgG activity as measured by the mixed agglutination test. In 21 of the 27 cases which could be completely studied, the anti-IgG activity was connected with the presence of IgA, either alone, or together with IgG or IgM, or both, and complement. All of these 21 biopsies showed morphologically active granulomatous arteritis with signs of tissue destruction. In 6 biopsies, the active component appeared to be some type of Fc receptor in the tissue. Morphologically these biopsies showed either non-granulomatous mononuclear arteritis without definite necrosis or they represented various stages of healing arteritis with no or minor signs of tissue destruction. Weak anti-IgG activity was often found in a morphological type characterized by minimal inflammatory activity. These lesions are easily overlooked and the mixed agglutination test proved to be a good diagnostic tool in such cases. Arteries without anti-IgG activity showed no signs of active arteritis.", "contents": "Immunological and histological studies of temporal arteries from patients with temporal arteritis and/or polymyalgia rheumatica. Biopsies from the temporal arteries of 62 out of 80 patients presenting the clinical picture of temporal arteritis and/or polymyalgia rheumatica showed morphologically active or healed arteritis. Fifty-five of these biopsies revealed anti-IgG activity as measured by the mixed agglutination test. In 21 of the 27 cases which could be completely studied, the anti-IgG activity was connected with the presence of IgA, either alone, or together with IgG or IgM, or both, and complement. All of these 21 biopsies showed morphologically active granulomatous arteritis with signs of tissue destruction. In 6 biopsies, the active component appeared to be some type of Fc receptor in the tissue. Morphologically these biopsies showed either non-granulomatous mononuclear arteritis without definite necrosis or they represented various stages of healing arteritis with no or minor signs of tissue destruction. Weak anti-IgG activity was often found in a morphological type characterized by minimal inflammatory activity. These lesions are easily overlooked and the mixed agglutination test proved to be a good diagnostic tool in such cases. Arteries without anti-IgG activity showed no signs of active arteritis."}
{"id": "PMID:1082710", "title": "Sequential studies of lymphocytes, neutrophils and serum proteins during prednisone treatment.", "content": "Seven patients (6 with connective tissue diseases, 1 with bronchial asthma) have been studied before, during, and after prednisone therapy. Maximum dose was 15 mg daily, which was tapered off to zero within three months. All patients showed striking subjective improvement during therapy. The ESR reflected this improvement but the acute phase proteins did not. The serum concentration of prealbumin rose significantly during the period of most intensive steroid treatment. IgE decreased in the patient with bronchial asthma, but otherwise the immunoglobulins did not change, and positive serological tests remained unchanged. Contact sensitization to haptens was induced without impairment during therapy. Prednisone induced rises in blood lymphocyte and neutrophil concentrations. Lymphocyte transformation, both mitogen- and antigen-induced, was not influenced by therapy, but PPD-induced inhibition of leucocyte migration decreased. Neutrophil phagocytosis was unimparied, but bactericidal capacity, stimulated nitroblue tetrazolium reduction, and neutrophil and plasma lysozyme concentrations were all depressed during treatment with prednisone.", "contents": "Sequential studies of lymphocytes, neutrophils and serum proteins during prednisone treatment. Seven patients (6 with connective tissue diseases, 1 with bronchial asthma) have been studied before, during, and after prednisone therapy. Maximum dose was 15 mg daily, which was tapered off to zero within three months. All patients showed striking subjective improvement during therapy. The ESR reflected this improvement but the acute phase proteins did not. The serum concentration of prealbumin rose significantly during the period of most intensive steroid treatment. IgE decreased in the patient with bronchial asthma, but otherwise the immunoglobulins did not change, and positive serological tests remained unchanged. Contact sensitization to haptens was induced without impairment during therapy. Prednisone induced rises in blood lymphocyte and neutrophil concentrations. Lymphocyte transformation, both mitogen- and antigen-induced, was not influenced by therapy, but PPD-induced inhibition of leucocyte migration decreased. Neutrophil phagocytosis was unimparied, but bactericidal capacity, stimulated nitroblue tetrazolium reduction, and neutrophil and plasma lysozyme concentrations were all depressed during treatment with prednisone."}
{"id": "PMID:1082711", "title": "Diagnosis and treatment of acute gastrointestinal haemorrhage in a small district hospital.", "content": "Ninety-eight consecutive patients admitted to a small district hospital because of acute gastrointestinal haemorrhage have been studied. Patients with haematemesis and/or melaena were treated with antacids and tranexamic acid from the very beginning and were examined with early panendscopy. All patients were closely observed in an ordinary medical ward by a staff especially trained to handle acute gastrointestinal bleeding. Seven patients required acute surgery. The overall mortality was 4.1%. It is concluded that acute gastrointestinal haemorrhage can be successfully handled with modern diagnosis and treatment in a small hospital.", "contents": "Diagnosis and treatment of acute gastrointestinal haemorrhage in a small district hospital. Ninety-eight consecutive patients admitted to a small district hospital because of acute gastrointestinal haemorrhage have been studied. Patients with haematemesis and/or melaena were treated with antacids and tranexamic acid from the very beginning and were examined with early panendscopy. All patients were closely observed in an ordinary medical ward by a staff especially trained to handle acute gastrointestinal bleeding. Seven patients required acute surgery. The overall mortality was 4.1%. It is concluded that acute gastrointestinal haemorrhage can be successfully handled with modern diagnosis and treatment in a small hospital."}
{"id": "PMID:1082713", "title": "Hemorrhagic diathesis in a carrier of hemophilia B.", "content": "A carrier of hemophilia B was found to have an unusually low factor IX level of 13 per cent. Her history of previous bleeding and the hospital course following elective dental extractions were consistent with a mild hemorrhagic diathesis. The patient is a member of a rare kindred of hemophiliacs. The mean level of factor IX in 12 carriers in this kindred was 42 per cent, with a range of 13 to 100 per cent. This patient represents the sixth reported case in which a female carrier of factor IX deficiency was symptomatic.", "contents": "Hemorrhagic diathesis in a carrier of hemophilia B. A carrier of hemophilia B was found to have an unusually low factor IX level of 13 per cent. Her history of previous bleeding and the hospital course following elective dental extractions were consistent with a mild hemorrhagic diathesis. The patient is a member of a rare kindred of hemophiliacs. The mean level of factor IX in 12 carriers in this kindred was 42 per cent, with a range of 13 to 100 per cent. This patient represents the sixth reported case in which a female carrier of factor IX deficiency was symptomatic."}
{"id": "PMID:1082714", "title": "Association of a chromosomal abnormality with lymphocytes having both T and B markers in a patient with lymphoproliferative disease.", "content": "The lymphocytes of a patient with leukemic lymphosarcoma were found to have an unusual surface phenotype in that they bound both sheep erythrocytes (a T cell marker) and complement-coated erythrocytes (a B cell marker) but lacked other B cell surface characteristics. Marker chromosomes were present in these cells, but not in other, phenotypically normal cells from the same patient. This case may provide a clue to the chromosomal origin of some lymphocyte surface markers in man.", "contents": "Association of a chromosomal abnormality with lymphocytes having both T and B markers in a patient with lymphoproliferative disease. The lymphocytes of a patient with leukemic lymphosarcoma were found to have an unusual surface phenotype in that they bound both sheep erythrocytes (a T cell marker) and complement-coated erythrocytes (a B cell marker) but lacked other B cell surface characteristics. Marker chromosomes were present in these cells, but not in other, phenotypically normal cells from the same patient. This case may provide a clue to the chromosomal origin of some lymphocyte surface markers in man."}
{"id": "PMID:1082716", "title": "Further observations on the diagnosis cause, and treatment of endophthalmitis.", "content": "In our study of 54 suspected cases of endophthalmitis, vitreous aspiration was more sensitive in making a culture-proven diagnosis than anterior chamber paracentesis; Staphylococcus epidermidis was a more common cause of endophthalmitis than previously appreciated; and intraocular antibiotics in the recommended dosage are reasonably safe clinically and add a new dimension to the treatment of endophthalmitis.", "contents": "Further observations on the diagnosis cause, and treatment of endophthalmitis. In our study of 54 suspected cases of endophthalmitis, vitreous aspiration was more sensitive in making a culture-proven diagnosis than anterior chamber paracentesis; Staphylococcus epidermidis was a more common cause of endophthalmitis than previously appreciated; and intraocular antibiotics in the recommended dosage are reasonably safe clinically and add a new dimension to the treatment of endophthalmitis."}
{"id": "PMID:1082717", "title": "Simultaneous bacterial endophthalmitis and sympathetic uveitis after retinal detachment surgery.", "content": "In a 66-year-old white woman suppurative endophthalmitis and sympathetic uveitis developed simultaneously about eight months after conventional repair of a rhegmatogenous retinal detachment. Histopathologic findings included vitreous abscess with probable staphylococcus organisms, total retinal detechment and diffuse chronic choroiditis with granulomatous foci, typical of sympathetic uveitis.", "contents": "Simultaneous bacterial endophthalmitis and sympathetic uveitis after retinal detachment surgery. In a 66-year-old white woman suppurative endophthalmitis and sympathetic uveitis developed simultaneously about eight months after conventional repair of a rhegmatogenous retinal detachment. Histopathologic findings included vitreous abscess with probable staphylococcus organisms, total retinal detechment and diffuse chronic choroiditis with granulomatous foci, typical of sympathetic uveitis."}
{"id": "PMID:1082718", "title": "Binocular enhancement of color discrimination in a deutan.", "content": "A 31-year-old white male deutan produced reliably different profiles when examined binocularly and monocularly with a Farnsworth-Munsell 100-hue test. Discrimination in the long wavelengths improved under the binocular conditions. Intensive testing yielded no information to account for the phenomenon.", "contents": "Binocular enhancement of color discrimination in a deutan. A 31-year-old white male deutan produced reliably different profiles when examined binocularly and monocularly with a Farnsworth-Munsell 100-hue test. Discrimination in the long wavelengths improved under the binocular conditions. Intensive testing yielded no information to account for the phenomenon."}
{"id": "PMID:1082719", "title": "Necrotic myelopathy (myelomalacia) in rats with allergic encephalomyelitis treated with tilorone.", "content": "Necrosis of the spinal cord was produced by administering tilorone to rats before or during the incubation period of experimental allergic encephalomyelitis (EAE). Under slected conditions of dose and timing, the drug delayed onset of clinical signs but did not prevent progression to paralysis. The lymphocytic component of the inflammatory lesions was reduced, but this was accompanied by a dramatic increase of monocytes in the spinal cord, followed by softening (myelomalacia). This new variant of EAE simulates necrotic myelopathy in man. The similarity provides support for an autoimmune etiology of the latter. Furthermore, the inverse relation between lymphocytic cuffs around vessels and massive monocytic infiltration of the cord adds to the growing evidence that lymphocytic cuffs protect the neural parenchyma by \"vascular blockade.\"", "contents": "Necrotic myelopathy (myelomalacia) in rats with allergic encephalomyelitis treated with tilorone. Necrosis of the spinal cord was produced by administering tilorone to rats before or during the incubation period of experimental allergic encephalomyelitis (EAE). Under slected conditions of dose and timing, the drug delayed onset of clinical signs but did not prevent progression to paralysis. The lymphocytic component of the inflammatory lesions was reduced, but this was accompanied by a dramatic increase of monocytes in the spinal cord, followed by softening (myelomalacia). This new variant of EAE simulates necrotic myelopathy in man. The similarity provides support for an autoimmune etiology of the latter. Furthermore, the inverse relation between lymphocytic cuffs around vessels and massive monocytic infiltration of the cord adds to the growing evidence that lymphocytic cuffs protect the neural parenchyma by \"vascular blockade.\""}
{"id": "PMID:1082720", "title": "Elastic modulus and stress relationships in stretched and shortened frog sartorii.", "content": "The longitudinal dynamic elastic modulus (delta stress/delta strain) of 11 sartorius muscles (Rana pipiens; 3 degrees C) was measured at static strains, 0.57 less than L/Lo less than 1.53. Pseudorandom white-noise displacements less than +/-0.031% Lo (mean, peak to peak) were imposed on tetanically stimulated and resting muscles to obtain the isometric moduli. The active elastic modulus at each length was determined as the difference between the low-frequency (15-80 Hz) asymptotes of the tetanic and resting modulus functions. Resting moduli were found to increase with stretch at a declining rate, suggesting that some resting elasticity is attributable to active crossbridges. For isometric, tetanically stimulated muscles above L/Lo = 1.0, the ratio dynamic elastic modulus/active stress was nearly constant (65.4); the data predict zero modulus at a stretched length corresponding to zero active stress. Hence, the modulus per crossbridge exhibits sarcomere length invariance. On the other hand, active muscles below L/Lo = 0.77 manifest a significant (P less than or equal to 0.05) additional modulus beyond that found at the same active stress in the stretched muscle. Sarcomeric structural rearrangements are suggested as a possible source of this additional modulus.", "contents": "Elastic modulus and stress relationships in stretched and shortened frog sartorii. The longitudinal dynamic elastic modulus (delta stress/delta strain) of 11 sartorius muscles (Rana pipiens; 3 degrees C) was measured at static strains, 0.57 less than L/Lo less than 1.53. Pseudorandom white-noise displacements less than +/-0.031% Lo (mean, peak to peak) were imposed on tetanically stimulated and resting muscles to obtain the isometric moduli. The active elastic modulus at each length was determined as the difference between the low-frequency (15-80 Hz) asymptotes of the tetanic and resting modulus functions. Resting moduli were found to increase with stretch at a declining rate, suggesting that some resting elasticity is attributable to active crossbridges. For isometric, tetanically stimulated muscles above L/Lo = 1.0, the ratio dynamic elastic modulus/active stress was nearly constant (65.4); the data predict zero modulus at a stretched length corresponding to zero active stress. Hence, the modulus per crossbridge exhibits sarcomere length invariance. On the other hand, active muscles below L/Lo = 0.77 manifest a significant (P less than or equal to 0.05) additional modulus beyond that found at the same active stress in the stretched muscle. Sarcomeric structural rearrangements are suggested as a possible source of this additional modulus."}
{"id": "PMID:1082721", "title": "The family and schizophrenia.", "content": "The author discusses the role of the family in the etiology of schizophrenia and suggests how this research might be integrated with our growing knowledge of the biology of schizophrenia. Abnormalities of family interaction-particularly communication deviances-are frequently associated with schizophrenia and may play a causal, rather than epiphenomenal, role in its pathogenesis. Communication processes in family life affect the child's development of attentional and perceptual capacities, which also have strong biologic roots. The author suggests that the study of normal and abnormal perceptual development might be a meeting ground for researchers in the areas of the biological and familial theories of schizophrenia.", "contents": "The family and schizophrenia. The author discusses the role of the family in the etiology of schizophrenia and suggests how this research might be integrated with our growing knowledge of the biology of schizophrenia. Abnormalities of family interaction-particularly communication deviances-are frequently associated with schizophrenia and may play a causal, rather than epiphenomenal, role in its pathogenesis. Communication processes in family life affect the child's development of attentional and perceptual capacities, which also have strong biologic roots. The author suggests that the study of normal and abnormal perceptual development might be a meeting ground for researchers in the areas of the biological and familial theories of schizophrenia."}
{"id": "PMID:1082723", "title": "Investigations on the influence of different corticosteroids on the development of galactose cataracts in rats.", "content": "The influence of daily subcutaneous injections of 5 different corticosteroids (15 mg/kg body weight) on the development of galactose opacities in young rats of approximately 60g body weight was investigated. Progress of cataracts was evaluated by planimetry of lens photos. The three glucocorticosteroids used (cortisol, prednisolone, fluocortolone) induced a slight acceleration of cataract development, while a mineralocorticoid (desoxy-corticosterone) caused a marked retardation. A temporary retardation was also observed with fluocortinbutyl, a rapidly metabolized steroid for topical use.", "contents": "Investigations on the influence of different corticosteroids on the development of galactose cataracts in rats. The influence of daily subcutaneous injections of 5 different corticosteroids (15 mg/kg body weight) on the development of galactose opacities in young rats of approximately 60g body weight was investigated. Progress of cataracts was evaluated by planimetry of lens photos. The three glucocorticosteroids used (cortisol, prednisolone, fluocortolone) induced a slight acceleration of cataract development, while a mineralocorticoid (desoxy-corticosterone) caused a marked retardation. A temporary retardation was also observed with fluocortinbutyl, a rapidly metabolized steroid for topical use."}
{"id": "PMID:1082724", "title": "[ERG in Beh\u00e7et's disease (author's transl)].", "content": "Rod- and cone-system electroretinograms from 16 patients suffering of recurrent posterior uveitis and one or more symptoms of Beh\u00e7et's disease were studied. On the basis of an evaluation of the clinical picture and of the progression three stages of the disease were distinguished. These stages were compared to the changes in amplitude of the b-waves of the ERGs (Fig 1). The findings in this group of patients were also compared to those from 11 patients with \"simple\" posterior uveitis (Fig. 2). The electroretinographic results in both these groups were highly similar: in all patients progression of the ocular disease was accompanied by parallel reduction in amplitude of the b-wave of the ERG. All ERG responses were affected in a similar way: the rod-system was in general more severly affected than the cone-system in both groups of patients. On the basis of these data it appears that the ERG indicates the stage of retinal damage in posterior uveitis. However, we could not detect ERG changes characteristic for the Beh\u00e7et type of uveitis.", "contents": "[ERG in Beh\u00e7et's disease (author's transl)]. Rod- and cone-system electroretinograms from 16 patients suffering of recurrent posterior uveitis and one or more symptoms of Beh\u00e7et's disease were studied. On the basis of an evaluation of the clinical picture and of the progression three stages of the disease were distinguished. These stages were compared to the changes in amplitude of the b-waves of the ERGs (Fig 1). The findings in this group of patients were also compared to those from 11 patients with \"simple\" posterior uveitis (Fig. 2). The electroretinographic results in both these groups were highly similar: in all patients progression of the ocular disease was accompanied by parallel reduction in amplitude of the b-wave of the ERG. All ERG responses were affected in a similar way: the rod-system was in general more severly affected than the cone-system in both groups of patients. On the basis of these data it appears that the ERG indicates the stage of retinal damage in posterior uveitis. However, we could not detect ERG changes characteristic for the Beh\u00e7et type of uveitis."}
{"id": "PMID:1082725", "title": "Ring-shaped alkali burns of the rabbit cornea. II. Early changes of the corneal stroma.", "content": "Corneas of 32 rabbits were coagulated in a circular zone of 1 mm width and 10 mm diameter. The results of the experiment showed sharply limited injury throughout the entire depth of the cornea without lateral diffusion. The reactions of the corneal stroma during the first three days are described and discussed.", "contents": "Ring-shaped alkali burns of the rabbit cornea. II. Early changes of the corneal stroma. Corneas of 32 rabbits were coagulated in a circular zone of 1 mm width and 10 mm diameter. The results of the experiment showed sharply limited injury throughout the entire depth of the cornea without lateral diffusion. The reactions of the corneal stroma during the first three days are described and discussed."}
{"id": "PMID:1082726", "title": "Experiments concerning the human C-wave.", "content": "The dependence of the human c-wave from the step amplitude was studied qualitatively. The maximum of the c-wave is achieved with light stimuli longer than 10 sec. After a dark period of 10 sec a fully developed c-wave appears. Also, in response to a brilliant photoflash a c-wave could be recorded. The influence of a mobile pupil on the response in the DC-ERG is demonstrated. Responses of the retinal potential to square wave and sinusoidal stimuli of different period times have been registered.", "contents": "Experiments concerning the human C-wave. The dependence of the human c-wave from the step amplitude was studied qualitatively. The maximum of the c-wave is achieved with light stimuli longer than 10 sec. After a dark period of 10 sec a fully developed c-wave appears. Also, in response to a brilliant photoflash a c-wave could be recorded. The influence of a mobile pupil on the response in the DC-ERG is demonstrated. Responses of the retinal potential to square wave and sinusoidal stimuli of different period times have been registered."}
{"id": "PMID:1082727", "title": "[The Mackay-Marg-tonometer. VII. The continuous registration of the intraocular pressure (author's transl)].", "content": "The Mackay-Marg-Tonometer registers continuously the intraocular pressure (Pt) as exactly as an electromanometer connected with the eye provided that the corneal rigidity is eliminitated by a smooth indentation of the cornea by the tip of the probe, the determined deviation of the registered values from linearity is considered and the temperature of the probe is not influenced from outside.", "contents": "[The Mackay-Marg-tonometer. VII. The continuous registration of the intraocular pressure (author's transl)]. The Mackay-Marg-Tonometer registers continuously the intraocular pressure (Pt) as exactly as an electromanometer connected with the eye provided that the corneal rigidity is eliminitated by a smooth indentation of the cornea by the tip of the probe, the determined deviation of the registered values from linearity is considered and the temperature of the probe is not influenced from outside."}
{"id": "PMID:1082728", "title": "A theoretical model for the eye of new-born infants.", "content": "A Gullstrand model for the eye of new-born infants is proposed, based on intra-ocular distances obtained by ultrasonography and lens radii measured on sections of deep-frozen preparations (v. Pflugk, 1909). Refractive indices of the media are assumed to be equal or nearly so to those of the adult eye, since no experimental data are available. The focal length of the model is 15.74 mm, the power 84.8 dptr, that of the lens alone 43.4 dptr, and the refractive error +2.8 dptr. A simplified model where the inner surface of the cornea is omitted, has a focal length of 15.63 mm, a power of 85.3 dptr and a refractive error of +2.4 dptr. When the data of v. Pflugk are analyzed statistically it is found that the spread of the lens power is considerably smaller than that which would be expected from the spread of the radii.", "contents": "A theoretical model for the eye of new-born infants. A Gullstrand model for the eye of new-born infants is proposed, based on intra-ocular distances obtained by ultrasonography and lens radii measured on sections of deep-frozen preparations (v. Pflugk, 1909). Refractive indices of the media are assumed to be equal or nearly so to those of the adult eye, since no experimental data are available. The focal length of the model is 15.74 mm, the power 84.8 dptr, that of the lens alone 43.4 dptr, and the refractive error +2.8 dptr. A simplified model where the inner surface of the cornea is omitted, has a focal length of 15.63 mm, a power of 85.3 dptr and a refractive error of +2.4 dptr. When the data of v. Pflugk are analyzed statistically it is found that the spread of the lens power is considerably smaller than that which would be expected from the spread of the radii."}
{"id": "PMID:1082729", "title": "[Changes of the phosphatides and their fatty acids in the retina and in the fasciculus opticus after retinal detachment: investigations of human and animal retinae (author's transl)].", "content": "In this study the effect of an experimentally provoked retinal detachment on the pattern of the phosphatides and fatty acids of the retina and the optic nerve of adult rabbits was investigated. The analysis was performed one month, and 4 months after the operation, and the values were related to the findings in control animals of the same age, and of the age of one day and 30 days respectively. In this way changes in the total lipide, in the phosphatides and in their fatty acids could be revealed, with a tendency towards the developmental stage of the 20th up to the 30th day of life. Between retina and optic nerve no difference was found neither temporally nor regarding the quantitative reaction. In the detached human retinae the same reactions can be proved as in the animal experiment. The relationship of these findings to the recovery of the operatively reatached human retina is discussed.", "contents": "[Changes of the phosphatides and their fatty acids in the retina and in the fasciculus opticus after retinal detachment: investigations of human and animal retinae (author's transl)]. In this study the effect of an experimentally provoked retinal detachment on the pattern of the phosphatides and fatty acids of the retina and the optic nerve of adult rabbits was investigated. The analysis was performed one month, and 4 months after the operation, and the values were related to the findings in control animals of the same age, and of the age of one day and 30 days respectively. In this way changes in the total lipide, in the phosphatides and in their fatty acids could be revealed, with a tendency towards the developmental stage of the 20th up to the 30th day of life. Between retina and optic nerve no difference was found neither temporally nor regarding the quantitative reaction. In the detached human retinae the same reactions can be proved as in the animal experiment. The relationship of these findings to the recovery of the operatively reatached human retina is discussed."}
{"id": "PMID:1082730", "title": "[Fructose levels of the aqueous humour after abrasio of the corneal epithelium (author's transl)].", "content": "Fructose and glucose levels of cornea stroma and aqueous humour were determined after abrasio of the corneal epithelium within five days. As the glucose levels remained, fructose levels were diminished significantly, when the epithelium was scraped off. The role of the corneal epithelium as a source of fructose in rabbit aqueous humour is discussed.", "contents": "[Fructose levels of the aqueous humour after abrasio of the corneal epithelium (author's transl)]. Fructose and glucose levels of cornea stroma and aqueous humour were determined after abrasio of the corneal epithelium within five days. As the glucose levels remained, fructose levels were diminished significantly, when the epithelium was scraped off. The role of the corneal epithelium as a source of fructose in rabbit aqueous humour is discussed."}
{"id": "PMID:1082731", "title": "Testicular reaction to prolonged exposure to nitrous oxide.", "content": "Male LEW/f Mai rats were exposed to an atmosphere of 20 per cent N2O, 20 per cent O2, and 60 per cent N2 for a maximum of 35 days. Evidence of injury to the seminiferous tubules was found in some animals by the second day. By 14 days, such damage was found in all animals. The toxic effect was confined to the spermatogenic cells, with consequent reduction in mature spermatozoa and appearance of multinucleated forms. Other cells within the testes were resistant to damage. Recovery of spermatogenesis occurred after return to room air for more than three days. Serum testosterone levels were not significantly affected during the prolonged exposure.", "contents": "Testicular reaction to prolonged exposure to nitrous oxide. Male LEW/f Mai rats were exposed to an atmosphere of 20 per cent N2O, 20 per cent O2, and 60 per cent N2 for a maximum of 35 days. Evidence of injury to the seminiferous tubules was found in some animals by the second day. By 14 days, such damage was found in all animals. The toxic effect was confined to the spermatogenic cells, with consequent reduction in mature spermatozoa and appearance of multinucleated forms. Other cells within the testes were resistant to damage. Recovery of spermatogenesis occurred after return to room air for more than three days. Serum testosterone levels were not significantly affected during the prolonged exposure."}
{"id": "PMID:1082732", "title": "Family distribution of lymphocytotoxins in Hodgkin's disease.", "content": "The prevalence of lymphocytotoxic antibody was studied in 131 relatives of 10 patients with Hodgkin's disease. The study group contained nine families, five of which had two subjects with Hodgkin's disease. One hundred twenty-eight control family members were studied in parallel. Lymphocytotoxic antibody was present in 35.5% of all family members of patients with Hodgkin's disease, and in 8.6% of controls (P less than 0.01). Lymphocytotoxic antibody appeared primarily in consanguineous relatives irrespective of close personal household contact with the probands. The prevalence of the antibody was equal in both first- and second-degree relatives. These findings suggest at least a genetic and possibly an environmental influence in the genesis of lymphocytotoxic antibody among relatives of patients with Hodgkin's disease.", "contents": "Family distribution of lymphocytotoxins in Hodgkin's disease. The prevalence of lymphocytotoxic antibody was studied in 131 relatives of 10 patients with Hodgkin's disease. The study group contained nine families, five of which had two subjects with Hodgkin's disease. One hundred twenty-eight control family members were studied in parallel. Lymphocytotoxic antibody was present in 35.5% of all family members of patients with Hodgkin's disease, and in 8.6% of controls (P less than 0.01). Lymphocytotoxic antibody appeared primarily in consanguineous relatives irrespective of close personal household contact with the probands. The prevalence of the antibody was equal in both first- and second-degree relatives. These findings suggest at least a genetic and possibly an environmental influence in the genesis of lymphocytotoxic antibody among relatives of patients with Hodgkin's disease."}
{"id": "PMID:1082737", "title": "Collateral circulation before and after coronary artery reconstruction.", "content": "The changes in collateral circulation after coronary artery reconstruction were analyzed in 75 patients with 177 bypass grafts and 18 complementary gas endarterectomies. The quantity of collateral circulation was directly proportional to the degree of coronary obstruction. The changes in collateral circulation depended on the success of coronary surgery or on the progression of the disease in coronary vessels. So, when the grafts remained patent, the collaterals disappeared and when the grafts became occluded, the collateral circulation returned to the preoperative level. Progressive obstruction of the coronaries caused increased collateral circulation.", "contents": "Collateral circulation before and after coronary artery reconstruction. The changes in collateral circulation after coronary artery reconstruction were analyzed in 75 patients with 177 bypass grafts and 18 complementary gas endarterectomies. The quantity of collateral circulation was directly proportional to the degree of coronary obstruction. The changes in collateral circulation depended on the success of coronary surgery or on the progression of the disease in coronary vessels. So, when the grafts remained patent, the collaterals disappeared and when the grafts became occluded, the collateral circulation returned to the preoperative level. Progressive obstruction of the coronaries caused increased collateral circulation."}
{"id": "PMID:1082735", "title": "[Stenoses caused by Murphy's button in the treatment of esophageal varices].", "content": "Treatment of oesophageal varices by insertion of Murphy's button arrests haemorrhage but in 2/3 of the cases of secondary stenosis occurs between the 4th and 8th week. The use of Blakemore's probe contributes to this. It is a very severe stenosis in 15 p. 100 of the cases and may require insertion of a wire loop. Dilatation should be started as soon as the first symptoms appear.", "contents": "[Stenoses caused by Murphy's button in the treatment of esophageal varices]. Treatment of oesophageal varices by insertion of Murphy's button arrests haemorrhage but in 2/3 of the cases of secondary stenosis occurs between the 4th and 8th week. The use of Blakemore's probe contributes to this. It is a very severe stenosis in 15 p. 100 of the cases and may require insertion of a wire loop. Dilatation should be started as soon as the first symptoms appear."}
{"id": "PMID:1082740", "title": "Coronary ostial stenosis complicating coronary arteriography.", "content": "Coronary ostial stenosis is a cause of angina pectoris and sudden death. It is due mainly to atherosclerosis, syphilis, and iatrogenic disease. The last is of growing importance because of the frequency of coronary arteriography and surgical procedures on the aortic valve. Since both may cause stenosis of the coronary ostia, these procedures raise the topic from an obscure morphologic entity to an important consideration in the treatment of cardiac disease. We describe a case of ostial stenosis that was complicated by coronary arteriography.", "contents": "Coronary ostial stenosis complicating coronary arteriography. Coronary ostial stenosis is a cause of angina pectoris and sudden death. It is due mainly to atherosclerosis, syphilis, and iatrogenic disease. The last is of growing importance because of the frequency of coronary arteriography and surgical procedures on the aortic valve. Since both may cause stenosis of the coronary ostia, these procedures raise the topic from an obscure morphologic entity to an important consideration in the treatment of cardiac disease. We describe a case of ostial stenosis that was complicated by coronary arteriography."}
{"id": "PMID:1082741", "title": "Systemic and local complications following low-friction arthroplasty of the hip joint. A study of 800 consecutive operations.", "content": "At the New York Orthopaedic Hospital, a study was made of postoperative complications in 800 consecutive total hip replacements of the Charnley low-friction arthroplasty type. Specially designed records made it possible to document and include all systemic and local complications of this procedure. While the rate of postoperative infection was low (less than 1%), thromboembolism and deep-vein thrombosis incidence was high despite the introduction of a prophylactic anticoagulation regimen. There were no deaths during or immediately after surgery, but six occurred within the first six weeks postoperatively. Two were due to a massive pulmonary embolus (proved at autopsy), two to myocardial infarction (clinical diagnosis), and one to a perforated cecum (proved at autopsy). The incidence of mechanical and technical failures in our series was relatively low and quite comparable with results reported by others.", "contents": "Systemic and local complications following low-friction arthroplasty of the hip joint. A study of 800 consecutive operations. At the New York Orthopaedic Hospital, a study was made of postoperative complications in 800 consecutive total hip replacements of the Charnley low-friction arthroplasty type. Specially designed records made it possible to document and include all systemic and local complications of this procedure. While the rate of postoperative infection was low (less than 1%), thromboembolism and deep-vein thrombosis incidence was high despite the introduction of a prophylactic anticoagulation regimen. There were no deaths during or immediately after surgery, but six occurred within the first six weeks postoperatively. Two were due to a massive pulmonary embolus (proved at autopsy), two to myocardial infarction (clinical diagnosis), and one to a perforated cecum (proved at autopsy). The incidence of mechanical and technical failures in our series was relatively low and quite comparable with results reported by others."}
{"id": "PMID:1082742", "title": "[The development OF THE vestibular apparatus under conditions of weightlessness].", "content": "The spawn of the aquarium fish Brachydanio rerio was developing during 5--6 days under conditions of weightlessness (first on board the spaceship \"Sojuz-16\", then in the space station \"Salut-4\") in special aquariums \"EMKON\", in thermostable installations. Electron microscopically the embryos were found to have a well developed labyrinth in early developmental histologically and cytologically differentiated receptory structures of the macula utriculi and macula saccili. In contrast to controls, the experimental animals showed certain alterations in the otolite organization. In similar experiments the embryos of clawed frog Xenopus laevis in the stage of the tail bud were also placed in special containers \"EMKON\" and thermostable apparatus \"Biotherm-4\" and by the spaceship \"Sojuz-17\" were brought to the space station \"Salut-4\", where it stayed for 16 days. The initial embryos had already had a well developed acoustic vesicle with macula communis. Inspite of the preliminary load by start acceleration and staying under conditions of weightlessness, they reached the general development fairly similar to controls. As it was shown electron microscopically their labyrinth had highly histologically and cytologically differentiated structures. However, a disturbance of the development of the otolithic membrane and otoconia should be noted. The alterations observed in the otolithic membrane organization in experimental fishes and frogs may be explained by general disorders in calcium metabolism.", "contents": "[The development OF THE vestibular apparatus under conditions of weightlessness]. The spawn of the aquarium fish Brachydanio rerio was developing during 5--6 days under conditions of weightlessness (first on board the spaceship \"Sojuz-16\", then in the space station \"Salut-4\") in special aquariums \"EMKON\", in thermostable installations. Electron microscopically the embryos were found to have a well developed labyrinth in early developmental histologically and cytologically differentiated receptory structures of the macula utriculi and macula saccili. In contrast to controls, the experimental animals showed certain alterations in the otolite organization. In similar experiments the embryos of clawed frog Xenopus laevis in the stage of the tail bud were also placed in special containers \"EMKON\" and thermostable apparatus \"Biotherm-4\" and by the spaceship \"Sojuz-17\" were brought to the space station \"Salut-4\", where it stayed for 16 days. The initial embryos had already had a well developed acoustic vesicle with macula communis. Inspite of the preliminary load by start acceleration and staying under conditions of weightlessness, they reached the general development fairly similar to controls. As it was shown electron microscopically their labyrinth had highly histologically and cytologically differentiated structures. However, a disturbance of the development of the otolithic membrane and otoconia should be noted. The alterations observed in the otolithic membrane organization in experimental fishes and frogs may be explained by general disorders in calcium metabolism."}
{"id": "PMID:1082738", "title": "Catecholamine-containing cells of the taste buds in the tongue of the frog (Rana temporaria).", "content": "In a study of the epithelial layer and taste buds of the tongue of the frog using fluorescence, peculiar dumb-bell shaped cells were found in the taste buds of fungiform papillae and these showed a highly-positive reaction to catecholamines. Adrenergic nerve fibres were detected in the vessels of the taste bud and in the epithelium of the tongue. Under conditions of catecholamine deficiency produced by preliminary reserpinization of frogs a decrease in specific fluorescence of the cells and the nerves was observed.", "contents": "Catecholamine-containing cells of the taste buds in the tongue of the frog (Rana temporaria). In a study of the epithelial layer and taste buds of the tongue of the frog using fluorescence, peculiar dumb-bell shaped cells were found in the taste buds of fungiform papillae and these showed a highly-positive reaction to catecholamines. Adrenergic nerve fibres were detected in the vessels of the taste bud and in the epithelium of the tongue. Under conditions of catecholamine deficiency produced by preliminary reserpinization of frogs a decrease in specific fluorescence of the cells and the nerves was observed."}
{"id": "PMID:1082743", "title": "[Changes in the dimensions of motor neurons and their components with different forms of nerve center activation].", "content": "Experiments in frogs have shown that a prolonged orthodromic rhythmical activation of motoneurons induce changes in their size and staining. Under these conditions the size of all components of motoneurons (cytoplasm, nucleus, nucleolus) is changed. The cytoplasm size changes are more pronounced than those of the nucleus. Under prolongation of the low frequency stimulation (1 per sec.) of the dorsal roots the changes are of phasic character: an initial enlargement (10 minute-long stimulation) and a subsequent decrease of all the above spinal cord motoneuron components (25 minute stimulation). After the 10 minute high frequency stimulation (50 impulses per second) of nerve centers there occur a sharp decrease of the size of the cytoplasm and the nucleus.", "contents": "[Changes in the dimensions of motor neurons and their components with different forms of nerve center activation]. Experiments in frogs have shown that a prolonged orthodromic rhythmical activation of motoneurons induce changes in their size and staining. Under these conditions the size of all components of motoneurons (cytoplasm, nucleus, nucleolus) is changed. The cytoplasm size changes are more pronounced than those of the nucleus. Under prolongation of the low frequency stimulation (1 per sec.) of the dorsal roots the changes are of phasic character: an initial enlargement (10 minute-long stimulation) and a subsequent decrease of all the above spinal cord motoneuron components (25 minute stimulation). After the 10 minute high frequency stimulation (50 impulses per second) of nerve centers there occur a sharp decrease of the size of the cytoplasm and the nucleus."}
{"id": "PMID:1082744", "title": "[Reactions of human neck muscle-fibers to vestibular stimulation (author's transl)].", "content": "In healthy human subjects activity from single fibers of the splenius-capitis muscle was recorded by means of a microelectrode. Two types of muscle fibers could be distinguished according to their reaction on vestibular stimulation. The first type reacted only to stimuli (angular acceleration) exceeding 10 degrees per sec2. These units showed no resting activity. The second type had a resting activity between 20 to 40 impulses per second. Their reactions under vestibular stimulation resembled that of primary afferent neurones of the vestibular nerve of other vertebrates. The functional meaning of these findings is discussed.", "contents": "[Reactions of human neck muscle-fibers to vestibular stimulation (author's transl)]. In healthy human subjects activity from single fibers of the splenius-capitis muscle was recorded by means of a microelectrode. Two types of muscle fibers could be distinguished according to their reaction on vestibular stimulation. The first type reacted only to stimuli (angular acceleration) exceeding 10 degrees per sec2. These units showed no resting activity. The second type had a resting activity between 20 to 40 impulses per second. Their reactions under vestibular stimulation resembled that of primary afferent neurones of the vestibular nerve of other vertebrates. The functional meaning of these findings is discussed."}
{"id": "PMID:1082736", "title": "[The facial nerve. Points of topographic anatomy].", "content": "The intracranial and basicranial pathways of the facial nerve present certain morphological peculiarities which are too often overlooked. These are considered to be unimportant details or simple abnormalities but are useful for the otologist to know when using a microscope for increasingly complicated surgical techniques.", "contents": "[The facial nerve. Points of topographic anatomy]. The intracranial and basicranial pathways of the facial nerve present certain morphological peculiarities which are too often overlooked. These are considered to be unimportant details or simple abnormalities but are useful for the otologist to know when using a microscope for increasingly complicated surgical techniques."}
{"id": "PMID:1082745", "title": "Mechanical explanation of the on-off effect (diphasic impedance change) in otospongiosis.", "content": "Impedancemetry gives important information in otospongiosis, both for relative compliance and impedance audiometry. The detection of the stapedius reflex is facilitated by recruitment in some audiometric types of otospongiosis and enables us to solve many of the masking problems which are particularly difficult in this disease due to the very important cross-hearing (or cross-over). Diphasic impedance changes (on-off effect) once detected must be systematically investigated since they allow us to detect otospongiosis early on. This sign is absolutely characteristic of the onset of a stapediovestibular involvement and is of primary interest in this disease because of its early appearance - before any classical audiometric change and any air-bone gap, i.e. before deafness. Diphasic impedance changes must thus be systematically investigated in case of so-called unilateral otospongiosis, in the audiometrically normal ear, and for the detection of otospongiosis in otospongiotic families. It is also helpful in confirming the otospongiotic origin of sensorineural losses before an air-bone gap appears. Our experience with routine stapedectomies and impedance audiometry measurements have led us to a purely mechanical explanation of the on-off effect, i.e. the response of an elastic system to a deformation with a different mechanism of the two inverse peaks, with a different mass and thus a different inertia. This mechanical explanation fits perfectly with anatomical findings. Everything occurs as if the evolutive stages of the onset of the stapediovestibular involvement were defined in impedancemetry, starting from the normal stapedius reflex, first by mixed forms, then by a pure on-off effect, finally by the disappearance of the stapedius reflex.", "contents": "Mechanical explanation of the on-off effect (diphasic impedance change) in otospongiosis. Impedancemetry gives important information in otospongiosis, both for relative compliance and impedance audiometry. The detection of the stapedius reflex is facilitated by recruitment in some audiometric types of otospongiosis and enables us to solve many of the masking problems which are particularly difficult in this disease due to the very important cross-hearing (or cross-over). Diphasic impedance changes (on-off effect) once detected must be systematically investigated since they allow us to detect otospongiosis early on. This sign is absolutely characteristic of the onset of a stapediovestibular involvement and is of primary interest in this disease because of its early appearance - before any classical audiometric change and any air-bone gap, i.e. before deafness. Diphasic impedance changes must thus be systematically investigated in case of so-called unilateral otospongiosis, in the audiometrically normal ear, and for the detection of otospongiosis in otospongiotic families. It is also helpful in confirming the otospongiotic origin of sensorineural losses before an air-bone gap appears. Our experience with routine stapedectomies and impedance audiometry measurements have led us to a purely mechanical explanation of the on-off effect, i.e. the response of an elastic system to a deformation with a different mechanism of the two inverse peaks, with a different mass and thus a different inertia. This mechanical explanation fits perfectly with anatomical findings. Everything occurs as if the evolutive stages of the onset of the stapediovestibular involvement were defined in impedancemetry, starting from the normal stapedius reflex, first by mixed forms, then by a pure on-off effect, finally by the disappearance of the stapedius reflex."}
{"id": "PMID:1082746", "title": "Perception of static orientation in a constant gravitoinertial environment.", "content": "The illusions associated with the perception of static tilt in various specific force environments have been reviewed and then classified in such a way that a simple perceptual model could be developed to account for the experimental data. The fundamental conclusion to be drawn from this model is that these illusions can be accounted for by a simple non-linear transformation of the information primarily from the saccule.", "contents": "Perception of static orientation in a constant gravitoinertial environment. The illusions associated with the perception of static tilt in various specific force environments have been reviewed and then classified in such a way that a simple perceptual model could be developed to account for the experimental data. The fundamental conclusion to be drawn from this model is that these illusions can be accounted for by a simple non-linear transformation of the information primarily from the saccule."}
{"id": "PMID:1082748", "title": "Electron microscopic observations of immunoreactive cells in the rheumatoid synovial membrane.", "content": "The perivascular infiltrates of the rheumatoid synovium were examined in the electron microscope. Lymphocyte-rich, plasma cell-rich, and transitional areas were observed. The transitional areas contained lymphocytes, plasma cells, blast cells, macrophages, and fribroblasts. Although lymphoblasts were frequent, the blast cells were predominantly plasmablasts. Marked degeneration of fibroblasts in the vicinity of lymphoblasts suggested the liberation of a lymphotoxin by the lymphoblasts. There was close contact between blast cells and macrophages and between macrophages and lymphocytes. The close association of lymphoblasts, plasmablasts, and macrophages in the transitional areas suggests that these are sites of T- and B-cell interaction in the rheumatoid synovial immune response.", "contents": "Electron microscopic observations of immunoreactive cells in the rheumatoid synovial membrane. The perivascular infiltrates of the rheumatoid synovium were examined in the electron microscope. Lymphocyte-rich, plasma cell-rich, and transitional areas were observed. The transitional areas contained lymphocytes, plasma cells, blast cells, macrophages, and fribroblasts. Although lymphoblasts were frequent, the blast cells were predominantly plasmablasts. Marked degeneration of fibroblasts in the vicinity of lymphoblasts suggested the liberation of a lymphotoxin by the lymphoblasts. There was close contact between blast cells and macrophages and between macrophages and lymphocytes. The close association of lymphoblasts, plasmablasts, and macrophages in the transitional areas suggests that these are sites of T- and B-cell interaction in the rheumatoid synovial immune response."}
{"id": "PMID:1082749", "title": "Cryoimmunoglobulinemia in Felty's syndrome.", "content": "Seven of 9 patients with Felty's syndrome exhibited cryoglobulins in greater amounts than found in uncomplicated RA but similar to amounts found in RA with vasculitis. Antinuclear antibodies were found in high titer in the sera and cryoglobulins of Felty's patients; when compared to SLE patients, whose sera and cryoglobulins also contained antinuclear factors, an antinuclear antibody reacting with nuclei of polymorphonuclear granulocytes appeared to be selectively concentrated in the cryoglobulins of some Felty's patients.", "contents": "Cryoimmunoglobulinemia in Felty's syndrome. Seven of 9 patients with Felty's syndrome exhibited cryoglobulins in greater amounts than found in uncomplicated RA but similar to amounts found in RA with vasculitis. Antinuclear antibodies were found in high titer in the sera and cryoglobulins of Felty's patients; when compared to SLE patients, whose sera and cryoglobulins also contained antinuclear factors, an antinuclear antibody reacting with nuclei of polymorphonuclear granulocytes appeared to be selectively concentrated in the cryoglobulins of some Felty's patients."}
{"id": "PMID:1082751", "title": "Cutaneous vessel immune deposits in rheumatoid arthritis.", "content": "A study to determine the presence of immune deposits in the cutaneous vessels of normal-appearing skin was made in 39 patients with rheumatoid arthritis. Immune deposits in skin vessels were found in 20 of the 32 patients with seropositive rheumatoid arthritis. The frequency of such immune deposits was only slightly higher in patients with clinical vasculitis than in those without such vasculitis. There was an association among the presence of immune deposits in skin vessels, presence of antinuclear antibodies, and elevated serum levels of IgM and IgA. The major immunoreactants detected were IgM and C3. The presence of immune deposits in skin vessels does not differentiate seropositive rheumatoid aptients who have clinical vasculitis from those who do not have clinical vasculitis.", "contents": "Cutaneous vessel immune deposits in rheumatoid arthritis. A study to determine the presence of immune deposits in the cutaneous vessels of normal-appearing skin was made in 39 patients with rheumatoid arthritis. Immune deposits in skin vessels were found in 20 of the 32 patients with seropositive rheumatoid arthritis. The frequency of such immune deposits was only slightly higher in patients with clinical vasculitis than in those without such vasculitis. There was an association among the presence of immune deposits in skin vessels, presence of antinuclear antibodies, and elevated serum levels of IgM and IgA. The major immunoreactants detected were IgM and C3. The presence of immune deposits in skin vessels does not differentiate seropositive rheumatoid aptients who have clinical vasculitis from those who do not have clinical vasculitis."}
{"id": "PMID:1082757", "title": "Stimulating factors in abnormal haematologic conditions.", "content": "Part of the present knowledge, due to the in vitro assays of human normal and leukaemic granulocytopoiesis, is briefly reviewed, with special attention to the meaning of the leukaemic CFU-C, and to the possible pathogenetic role of the relationship of CFU-C with CSF, in normal vs. leukaemic conditions. It is suggested that the role of CSF level during aplasia following cytotoxic therapy could be worthwhile of further investigation. Data are reported on the CSF activity of peripheral blood leukocytes in smouldering leukaemia (low), and in marrow aplasia (high), and of chronic myeloid leukaemia spleen cells (low CSF). Preliminary observations on the CFU-C content of spleen and liver in chronic myeloid leukaemia indicate that the role of these organs should receive adequate consideration.", "contents": "Stimulating factors in abnormal haematologic conditions. Part of the present knowledge, due to the in vitro assays of human normal and leukaemic granulocytopoiesis, is briefly reviewed, with special attention to the meaning of the leukaemic CFU-C, and to the possible pathogenetic role of the relationship of CFU-C with CSF, in normal vs. leukaemic conditions. It is suggested that the role of CSF level during aplasia following cytotoxic therapy could be worthwhile of further investigation. Data are reported on the CSF activity of peripheral blood leukocytes in smouldering leukaemia (low), and in marrow aplasia (high), and of chronic myeloid leukaemia spleen cells (low CSF). Preliminary observations on the CFU-C content of spleen and liver in chronic myeloid leukaemia indicate that the role of these organs should receive adequate consideration."}
{"id": "PMID:1082759", "title": "B-lymphocytosis factor in human plasma.", "content": "Patients with acute thermal burns experience an increase of serum immunoglobulins associated with marked B-lymphocytosis during the recovery phase from the burn injury. This study was performed to delineate humoral factors which may induce the B-lymphocytosis in such patients. Plasma samples were obtained serially from 14 adult patients and 14 adult controls. One-half ml of plasma was injected intraperitoneally into C3H/He male mice, and absolute numbers of surface immunoglobulin-bearing cells in the mouse peripheral blood were counted. Fractionation of plasma samples was performed. The biologically active plasma fraction, tentatively termed B-lymphocytosis factor (BLF), was cultured with normal human peripheral blood lymphocytes to determine in vitro lymphocyte transformation. The solubility of 125I-labelled BLF in various organic solvents was examined. Plasma obtained from burned patients induced a marked increase of IgG, IgA, and IgM-bearing cells in the peripheral blood of mice within 3 hours after injection. The B-lymphocytosis activity of normal plasma was not significant. The molecular weight of BLF was estimated to be close to that of ribonuclease A (M.W. 13,400). Transformation of normal human peripheral blood lymphocytes was observed when cultured with BLF. 125I-labelled BLF was not soluble in organic solvents. These data suggest that BLF is not a lipopolysaccharide, and plays a role in regulation of B-lymphocyte levels in burned patients.", "contents": "B-lymphocytosis factor in human plasma. Patients with acute thermal burns experience an increase of serum immunoglobulins associated with marked B-lymphocytosis during the recovery phase from the burn injury. This study was performed to delineate humoral factors which may induce the B-lymphocytosis in such patients. Plasma samples were obtained serially from 14 adult patients and 14 adult controls. One-half ml of plasma was injected intraperitoneally into C3H/He male mice, and absolute numbers of surface immunoglobulin-bearing cells in the mouse peripheral blood were counted. Fractionation of plasma samples was performed. The biologically active plasma fraction, tentatively termed B-lymphocytosis factor (BLF), was cultured with normal human peripheral blood lymphocytes to determine in vitro lymphocyte transformation. The solubility of 125I-labelled BLF in various organic solvents was examined. Plasma obtained from burned patients induced a marked increase of IgG, IgA, and IgM-bearing cells in the peripheral blood of mice within 3 hours after injection. The B-lymphocytosis activity of normal plasma was not significant. The molecular weight of BLF was estimated to be close to that of ribonuclease A (M.W. 13,400). Transformation of normal human peripheral blood lymphocytes was observed when cultured with BLF. 125I-labelled BLF was not soluble in organic solvents. These data suggest that BLF is not a lipopolysaccharide, and plays a role in regulation of B-lymphocyte levels in burned patients."}
{"id": "PMID:1082754", "title": "Lymphocyte responsiveness in systemic lupus erythematosus.", "content": "Lymphocyte transformation to various mitogens and antogens was studied in selected patients with systemic lupus erythematosus. These patients were selected because they had taken no drug other than low-dose aspirin for 2 months prior to presentation. An impaired transformation response to the mitogens phytohemagglutinin and pokeweed was found. There was no impairment in the response to PPD and Candida. The impaired mitogen response, which could not be corrected using purified, warm-washed, and warm-cultured T lymphocytes, seemed to be a corollary of disease activity.", "contents": "Lymphocyte responsiveness in systemic lupus erythematosus. Lymphocyte transformation to various mitogens and antogens was studied in selected patients with systemic lupus erythematosus. These patients were selected because they had taken no drug other than low-dose aspirin for 2 months prior to presentation. An impaired transformation response to the mitogens phytohemagglutinin and pokeweed was found. There was no impairment in the response to PPD and Candida. The impaired mitogen response, which could not be corrected using purified, warm-washed, and warm-cultured T lymphocytes, seemed to be a corollary of disease activity."}
{"id": "PMID:1082766", "title": "Left ventricular angiography on exercise. A new method of assessing left ventricular function in ischaemic heart disease.", "content": "Left ventricular function was studied in 17 patients with ischaemic heart disease and compared with 4 patients with normal left ventricular function. The patients in the homogeneous group of ischaemic heart disease were further subdivided into those 'without angina' (n=5) and those 'with angina' (n=12), depending upon the presence of angina during supine leg exercise at the time of definitive study. At rest there was no significant difference in the heart rate, cardiac output, stroke volume, and left ventricular end-diastolic pressure (LVEDP) in the three groups. During exercise the cardiac output and stroke volume were significantly depressed and LVEDP was significantly raised in the ischaemic heart disease group as a whole but within this group failed to show any significant difference in patients with and without angina. The left ventricular end-diastolic volume (LVEDV) and end-systolic volume (LVESV) measurements showed clear separation of these three groups only on exercise. On exercise, there was decrease in LVEDV and LVESV (P less than 0.05; P less than 0.02) in the group with normal left ventricular function, no change in the group with ischaemic heart disease without angina, and striking increase in LVEDV and LVESV in the group with ischaemic heart disease and angina (P less than 0.01 and P less than 0.02, respectively). This angiographic method of assessing left ventricular function shows clear separation of the three groups and also highlights the significance of angina. Ejection fraction (EF), a commonly measured parameter of left ventricular function, failed to reflect consistent changes on exercise as compared to values at rest which emphasizes the limitations of the measurement of ejection fraction at rest.", "contents": "Left ventricular angiography on exercise. A new method of assessing left ventricular function in ischaemic heart disease. Left ventricular function was studied in 17 patients with ischaemic heart disease and compared with 4 patients with normal left ventricular function. The patients in the homogeneous group of ischaemic heart disease were further subdivided into those 'without angina' (n=5) and those 'with angina' (n=12), depending upon the presence of angina during supine leg exercise at the time of definitive study. At rest there was no significant difference in the heart rate, cardiac output, stroke volume, and left ventricular end-diastolic pressure (LVEDP) in the three groups. During exercise the cardiac output and stroke volume were significantly depressed and LVEDP was significantly raised in the ischaemic heart disease group as a whole but within this group failed to show any significant difference in patients with and without angina. The left ventricular end-diastolic volume (LVEDV) and end-systolic volume (LVESV) measurements showed clear separation of these three groups only on exercise. On exercise, there was decrease in LVEDV and LVESV (P less than 0.05; P less than 0.02) in the group with normal left ventricular function, no change in the group with ischaemic heart disease without angina, and striking increase in LVEDV and LVESV in the group with ischaemic heart disease and angina (P less than 0.01 and P less than 0.02, respectively). This angiographic method of assessing left ventricular function shows clear separation of the three groups and also highlights the significance of angina. Ejection fraction (EF), a commonly measured parameter of left ventricular function, failed to reflect consistent changes on exercise as compared to values at rest which emphasizes the limitations of the measurement of ejection fraction at rest."}
{"id": "PMID:1082767", "title": "Immunological responses of patients with psoriasis and the effect of treatment with methotrexate.", "content": "A group of thirty-six patients of whom fourteen were being treated with methotrexate, were studied in order to assess T-lymphocyte function by in vitro techniques. Circulating T-lymphocytes in aliqots of blood were assessed by the rosetting technique. No differences were found in psoriatics, whether on methotrexate or not, compared with fifteen control subjects. Lymphocyte counts and lymphocyte transformation to phytohaemagglutinin (PHA) tended to be lower in the psoriatic group as a whole than in the controls, but the differences were not statistically significant, However, a significant inverse relationship was found between the extent of the skin lesions and lymphocyte transformation to PHA, i.e. the smaller the area of skin affected the higher the lymphocyte transformation. Psoriatics treated with methotrexate had fewer skin lesions and higher lymphocyte transformation to PHA than psoriatics not so treated, probably reflecting this inverse relationship. The reason why the presence of extensive psoriasis is associated with depressed lymphocyte transformation is not understood. No evidence was found that methotrexate depressed cell-mediated immunity as judged by these in vitro tests.", "contents": "Immunological responses of patients with psoriasis and the effect of treatment with methotrexate. A group of thirty-six patients of whom fourteen were being treated with methotrexate, were studied in order to assess T-lymphocyte function by in vitro techniques. Circulating T-lymphocytes in aliqots of blood were assessed by the rosetting technique. No differences were found in psoriatics, whether on methotrexate or not, compared with fifteen control subjects. Lymphocyte counts and lymphocyte transformation to phytohaemagglutinin (PHA) tended to be lower in the psoriatic group as a whole than in the controls, but the differences were not statistically significant, However, a significant inverse relationship was found between the extent of the skin lesions and lymphocyte transformation to PHA, i.e. the smaller the area of skin affected the higher the lymphocyte transformation. Psoriatics treated with methotrexate had fewer skin lesions and higher lymphocyte transformation to PHA than psoriatics not so treated, probably reflecting this inverse relationship. The reason why the presence of extensive psoriasis is associated with depressed lymphocyte transformation is not understood. No evidence was found that methotrexate depressed cell-mediated immunity as judged by these in vitro tests."}
{"id": "PMID:1082768", "title": "Langerhans cells in the epidermis of athymic mice.", "content": "Langerhans cells were identified with the electron microscope in the epidermis of six athymic nu/nu mice. Their origin in the thymus is ruled out and their functional dependence on either the thymus or T lymphocytes seems improbable.", "contents": "Langerhans cells in the epidermis of athymic mice. Langerhans cells were identified with the electron microscope in the epidermis of six athymic nu/nu mice. Their origin in the thymus is ruled out and their functional dependence on either the thymus or T lymphocytes seems improbable."}
{"id": "PMID:1082769", "title": "Lymphocyte abnormalities in mycosis fungoides.", "content": "Fourteen patients with clinical and histological evidence of mycosis fungoides have undergone a series of immunological tests. Significant findings include the presence of low numbers of E and EAC rosette-forming cells in the peripheral circulation of the mycosis fungoides patients. Elevated levels of IgE were seen in five of the mycosis fungoides patients, and the mean IgE level was significantly higher than in the control series. Possible explanations of these findings are considered.", "contents": "Lymphocyte abnormalities in mycosis fungoides. Fourteen patients with clinical and histological evidence of mycosis fungoides have undergone a series of immunological tests. Significant findings include the presence of low numbers of E and EAC rosette-forming cells in the peripheral circulation of the mycosis fungoides patients. Elevated levels of IgE were seen in five of the mycosis fungoides patients, and the mean IgE level was significantly higher than in the control series. Possible explanations of these findings are considered."}
{"id": "PMID:1082771", "title": "The scanning electron microscopy of normal human peripheral blood lymphocytes.", "content": "A study of the effects of various sample preparation techniques for scanning electron microscopy has been undertaken in an attempt to resolve conflicting descriptions of the surface topography of human peripheral blood lymphocytes. By fixing cells in suspension--a technique thought most likely to avoid the production of artefacts--no clearly defined morphological classification of lymphocytes could be made, and when T- and B-lymphocyte enriched preparations were studied their surfaces appeared similar. Both T- and B-rosetted cells showed identical morphological changes as a result of their interaction with red blood cells. The smooth cells described in other reports were found only under certain conditions of preparation. It is therefore not possible to distinguish between T- and B-cell populations, using the S.E.M., on the basis of surface morphology alone.", "contents": "The scanning electron microscopy of normal human peripheral blood lymphocytes. A study of the effects of various sample preparation techniques for scanning electron microscopy has been undertaken in an attempt to resolve conflicting descriptions of the surface topography of human peripheral blood lymphocytes. By fixing cells in suspension--a technique thought most likely to avoid the production of artefacts--no clearly defined morphological classification of lymphocytes could be made, and when T- and B-lymphocyte enriched preparations were studied their surfaces appeared similar. Both T- and B-rosetted cells showed identical morphological changes as a result of their interaction with red blood cells. The smooth cells described in other reports were found only under certain conditions of preparation. It is therefore not possible to distinguish between T- and B-cell populations, using the S.E.M., on the basis of surface morphology alone."}
{"id": "PMID:1082772", "title": "Effects of inhibitors of protein and RNA synthesis on aldosterone-stimulated changes in phospholipid fatty acid metabolism in the toad urinary bladder.", "content": "The effects of an inhibitor of RNA synthesis, cordycepin, and an inhibitor of protein synthesis, cycloheximide, on aldosterone-induced changes in lipid metabolism and phospholipid fatty acid composition have been studied in the toad urinary bladder. At the concentrations employed, the inhibitors abolish the hormone-induced increases in total lipid synthesis, phospholipid fatty acid specific activities, and weight percentage of phospholipid long-chain polyunsaturated fatty acids as well as blocking the aldosterone-mediated increase in sodium transport.", "contents": "Effects of inhibitors of protein and RNA synthesis on aldosterone-stimulated changes in phospholipid fatty acid metabolism in the toad urinary bladder. The effects of an inhibitor of RNA synthesis, cordycepin, and an inhibitor of protein synthesis, cycloheximide, on aldosterone-induced changes in lipid metabolism and phospholipid fatty acid composition have been studied in the toad urinary bladder. At the concentrations employed, the inhibitors abolish the hormone-induced increases in total lipid synthesis, phospholipid fatty acid specific activities, and weight percentage of phospholipid long-chain polyunsaturated fatty acids as well as blocking the aldosterone-mediated increase in sodium transport."}
{"id": "PMID:1082773", "title": "Ultra-slow inactivation of the ionic currents through the membrane of myelinated nerve.", "content": "(1) Voltage-clamp experiments were performed with myelinated fibres isolated from the sciatic nerve of the frog to study slow changes of the specific sodium and potassium currents as a function of membrane (holding) potential and time. (2) The level of the peak sodium current depends on holding potential VH. This dependence can be described by a sigmoidal function uinfinity(VH). The underlying process is called \"ultra-slow sodium inactivation\" and is different and separable from the short time steady-state inactivation, hinfinity(V), and from the slow inactivation depending on the extracellular potassium concentration (Adelman, Jr., W. J. and Palti, Y. (1969), J Gen. Physiol. 54, 589-606; Peganov, E. M., Khodorov, B.I. and Shishkova, L. D. (1973), Bull. Exp. Biol. Med. 25, 15-19; Khodorov, B. I. Shishkova, L. D. and Peganov, E. M. (1974), Bull. Exp. Biol. Med. 3, 10-14). (3) After a sudden change of the holding potential the sodium current reaches a new steady-state level (due to the transition of uinfinity(VH) to the corresponding value) within approx. 4 min. The kinetics of the transition cannot be described by a single exponential function. (4) A corresponding voltage- and time-dependent process of ultra-slow inactivation exists for the potassium current in the node of Ranvier. The kinetics are faster than those of the sodium system.", "contents": "Ultra-slow inactivation of the ionic currents through the membrane of myelinated nerve. (1) Voltage-clamp experiments were performed with myelinated fibres isolated from the sciatic nerve of the frog to study slow changes of the specific sodium and potassium currents as a function of membrane (holding) potential and time. (2) The level of the peak sodium current depends on holding potential VH. This dependence can be described by a sigmoidal function uinfinity(VH). The underlying process is called \"ultra-slow sodium inactivation\" and is different and separable from the short time steady-state inactivation, hinfinity(V), and from the slow inactivation depending on the extracellular potassium concentration (Adelman, Jr., W. J. and Palti, Y. (1969), J Gen. Physiol. 54, 589-606; Peganov, E. M., Khodorov, B.I. and Shishkova, L. D. (1973), Bull. Exp. Biol. Med. 25, 15-19; Khodorov, B. I. Shishkova, L. D. and Peganov, E. M. (1974), Bull. Exp. Biol. Med. 3, 10-14). (3) After a sudden change of the holding potential the sodium current reaches a new steady-state level (due to the transition of uinfinity(VH) to the corresponding value) within approx. 4 min. The kinetics of the transition cannot be described by a single exponential function. (4) A corresponding voltage- and time-dependent process of ultra-slow inactivation exists for the potassium current in the node of Ranvier. The kinetics are faster than those of the sodium system."}
{"id": "PMID:1082776", "title": "Effect of conditioning potential on potassium current kinetics in the frog node.", "content": "The kinetics of potassium conductance changes were determined in the voltage clamped frog node (Rana esculenta), as a function of conditioning prepotential. The conditioning potential duration varied from 1 to 50 ms and the amplitude between -60 and +130 mV (relative to rest). The conductance kinetics were determined at a single test potential of +20 mV (depolarization) by means of the slope of log [ninfinity - nt] vs. time relationship which defines the time constant of the process (tau). The values of tau, after conditioning hyperpolarizations, were around 5 ms, up to 10 times greater than values obtained following a strong depolarization. The tau vs. pre-potential curve was sigmoid in shape. These differences were only slightly dependent on [K+]0 or conditioning pulse duration. The steady-state current values were also found to be a function of conditioning potential. After conditioning hyperpolarizations, the log [ninfinity - nt] vs. time curve could not be fitted by a single exponent regardless of the power of n chosen. The prepotential dependency of potassium current kinetics is inconsistent with the Hodgkin-Huxley axon model where the conductance parameters are assumed to be in either one of two possible states, and where the rate of transfer from one state to the other follows first order kinetics. In contrast the described kinetics may be consistent with complex multistate potassium \"channel\" models or membranes consisting of a number of types of channels.", "contents": "Effect of conditioning potential on potassium current kinetics in the frog node. The kinetics of potassium conductance changes were determined in the voltage clamped frog node (Rana esculenta), as a function of conditioning prepotential. The conditioning potential duration varied from 1 to 50 ms and the amplitude between -60 and +130 mV (relative to rest). The conductance kinetics were determined at a single test potential of +20 mV (depolarization) by means of the slope of log [ninfinity - nt] vs. time relationship which defines the time constant of the process (tau). The values of tau, after conditioning hyperpolarizations, were around 5 ms, up to 10 times greater than values obtained following a strong depolarization. The tau vs. pre-potential curve was sigmoid in shape. These differences were only slightly dependent on [K+]0 or conditioning pulse duration. The steady-state current values were also found to be a function of conditioning potential. After conditioning hyperpolarizations, the log [ninfinity - nt] vs. time curve could not be fitted by a single exponent regardless of the power of n chosen. The prepotential dependency of potassium current kinetics is inconsistent with the Hodgkin-Huxley axon model where the conductance parameters are assumed to be in either one of two possible states, and where the rate of transfer from one state to the other follows first order kinetics. In contrast the described kinetics may be consistent with complex multistate potassium \"channel\" models or membranes consisting of a number of types of channels."}
{"id": "PMID:1082777", "title": "[Determination of choleragen activity in tadpoles].", "content": "A method of titration of the V. cholerae toxin obtained from the broth culture filtrates is presented. In difference from the earlier methods, the larvae of Rana temporaria were used as a test object. The tadpoles were placed in the vessels with water containing graded concentrations of the toxin. The titer of the toxin activity was evaluated by the percentage of dead tadpoles. The concentration causing the death of 50 per cent of the tadpoles in the course of the 24 hours was taken as a unit of toxin activity. The method is simple, easily reproducible and economical.", "contents": "[Determination of choleragen activity in tadpoles]. A method of titration of the V. cholerae toxin obtained from the broth culture filtrates is presented. In difference from the earlier methods, the larvae of Rana temporaria were used as a test object. The tadpoles were placed in the vessels with water containing graded concentrations of the toxin. The titer of the toxin activity was evaluated by the percentage of dead tadpoles. The concentration causing the death of 50 per cent of the tadpoles in the course of the 24 hours was taken as a unit of toxin activity. The method is simple, easily reproducible and economical."}
{"id": "PMID:1082778", "title": "Improved techniques for liquid culture of human and mouse bone marrow.", "content": "Previous studies using the in vitro diffusion chamber (Marbrook) have shown that bone marrow grown in this system will undergo limited stem cell replication and differentiation to mature granulocytes and mononuclear phagocytes. A series of studies with modified culture systems was initiated to improve cell production and committed stem cell (CFU-C) proliferation in vitro. Introduction of a continuous-flow system and a migration technique providing means of egress for mature neutrophils resulted in substantially improved performance. CFU-C were found to be capable of migration through a 3-mu pore membrane. These studies indicated that membrane surface area, culture medium circulation, and mature cell egress were among the conditions that could be optimized for maximum hematopoietic cell proliferation in suspension culture. The present observations also suggested that large-scale in vitro growth of mammalian bone marrow may be feasible.", "contents": "Improved techniques for liquid culture of human and mouse bone marrow. Previous studies using the in vitro diffusion chamber (Marbrook) have shown that bone marrow grown in this system will undergo limited stem cell replication and differentiation to mature granulocytes and mononuclear phagocytes. A series of studies with modified culture systems was initiated to improve cell production and committed stem cell (CFU-C) proliferation in vitro. Introduction of a continuous-flow system and a migration technique providing means of egress for mature neutrophils resulted in substantially improved performance. CFU-C were found to be capable of migration through a 3-mu pore membrane. These studies indicated that membrane surface area, culture medium circulation, and mature cell egress were among the conditions that could be optimized for maximum hematopoietic cell proliferation in suspension culture. The present observations also suggested that large-scale in vitro growth of mammalian bone marrow may be feasible."}
{"id": "PMID:1082774", "title": "[Model of the effect of the vestibular system on blood pressure during sleep].", "content": "A structural model of the effect of vestibular system on blood pressure during sleep is proposed. This model reflects joint action of the otolyth system and semicircular canal system, vestibular nuclei, vasomotor centre, vagus nuclei and of the central control mechanism of the state awakeness -- sleep. Anatomic and physiological prerequisites involved in the model are expressed by chains with transmission functions, signal generators, summators etc.", "contents": "[Model of the effect of the vestibular system on blood pressure during sleep]. A structural model of the effect of vestibular system on blood pressure during sleep is proposed. This model reflects joint action of the otolyth system and semicircular canal system, vestibular nuclei, vasomotor centre, vagus nuclei and of the central control mechanism of the state awakeness -- sleep. Anatomic and physiological prerequisites involved in the model are expressed by chains with transmission functions, signal generators, summators etc."}
{"id": "PMID:1082779", "title": "Production of colony-stimulating factor by leukemic leukocytes.", "content": "The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced normal or large quantities of CSF, while (with one exception) those from patients with myeloblastic leukemia produced little or no CSF. There was a general relationship between CSF production and serum lysozyme levels. Attempts to demonstrate a consistent inhibitory effect exerted by leukemic peripheral blood cells on normal leukopoiesis in vitro were negative. Results instead suggested that the addition to the feeder layer of cells from patients with monocytic leukemia could raise CSF levels above those obtained with normal peripheral blood leukocytes alone, possibly by recruiting additional CFUc from normal marrow.", "contents": "Production of colony-stimulating factor by leukemic leukocytes. The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced normal or large quantities of CSF, while (with one exception) those from patients with myeloblastic leukemia produced little or no CSF. There was a general relationship between CSF production and serum lysozyme levels. Attempts to demonstrate a consistent inhibitory effect exerted by leukemic peripheral blood cells on normal leukopoiesis in vitro were negative. Results instead suggested that the addition to the feeder layer of cells from patients with monocytic leukemia could raise CSF levels above those obtained with normal peripheral blood leukocytes alone, possibly by recruiting additional CFUc from normal marrow."}
{"id": "PMID:1082780", "title": "In vitro regulation of granulopoiesis in human leukemia: application of an assay for colony-inhibiting cells.", "content": "We describe two assays to detect the action of colony-inhibiting cells. In the first assay, we used a simple density separation technique to remove dense neutrophils (PMN) from suspensions of blood and of bone marrow cells prior to culture in semisolid agar. Conditions were arranged to ensure that control suspensions of unseparated cells and test suspensions of buoyant mononuclear cells differed only in their content of neutrophils. The control and test suspensions contained equal numbers of mononuclear cells (and granulocyte precursors). Colony (and cluster) formation was invariably enhanced in neutrophil-depleted cultures of normal cells. In the second assay, dense PMN, treated by an adherence separation procedure, were recovered, and the non-adherent dense PMN were added back to PMN-depleted cultures. A reproducible dose-related decrease in colony (and cluster) formation to basal levels resulted. The inhibitory effect was identical when the PMN were added directly to the culture (overlayer) or to the underlayer. In PMN-depleted cultures obtained from patients with leukemia and other hemopoietic disorders, neither colony nor cluster formation was enhanced, and sometimes it was reduced. When we compared the effect of adding patient and normal non-adherent PMN to target cultures of normal and patient PMN-depleted cells, some leukemic PMN were noninhibitory. Our results suggest that abnormalities of cellular interactions in vitro detected in the first assay may have more than one explanation, as shown when they are subjected to the closer scrutiny possible with the second assay.", "contents": "In vitro regulation of granulopoiesis in human leukemia: application of an assay for colony-inhibiting cells. We describe two assays to detect the action of colony-inhibiting cells. In the first assay, we used a simple density separation technique to remove dense neutrophils (PMN) from suspensions of blood and of bone marrow cells prior to culture in semisolid agar. Conditions were arranged to ensure that control suspensions of unseparated cells and test suspensions of buoyant mononuclear cells differed only in their content of neutrophils. The control and test suspensions contained equal numbers of mononuclear cells (and granulocyte precursors). Colony (and cluster) formation was invariably enhanced in neutrophil-depleted cultures of normal cells. In the second assay, dense PMN, treated by an adherence separation procedure, were recovered, and the non-adherent dense PMN were added back to PMN-depleted cultures. A reproducible dose-related decrease in colony (and cluster) formation to basal levels resulted. The inhibitory effect was identical when the PMN were added directly to the culture (overlayer) or to the underlayer. In PMN-depleted cultures obtained from patients with leukemia and other hemopoietic disorders, neither colony nor cluster formation was enhanced, and sometimes it was reduced. When we compared the effect of adding patient and normal non-adherent PMN to target cultures of normal and patient PMN-depleted cells, some leukemic PMN were noninhibitory. Our results suggest that abnormalities of cellular interactions in vitro detected in the first assay may have more than one explanation, as shown when they are subjected to the closer scrutiny possible with the second assay."}
{"id": "PMID:1082781", "title": "Relationship of colony-stimulating activity to apparent kill of human colony-forming cells by irradiation and hydroxyurea.", "content": "Suspensions of human bone marrow cells were subjected to 137Cs irradiation in vitro and then cultured in semisolid agar medium. Cultures of irradiated cells were stimulated with colony-stimulating activity (CSA) of different potencies, and it was found that the amount of stimulation applied to cultures influenced the apparent kill of colony-forming cells (CFC). It was also found that the effects of irradiation on colony formation were not confined to CFC kill since medium conditioned by cells during irradiation exhibited stimulatory and inhibitory properties after treatment by 600 and 1000 rads, respectively. Studies in which irradiated cells were pretreated with hydroxyurea indicated that CFC in the DNA synthetic phase of the cell cycle were particularly sensitive to low doses of irradiation. The proliferative capacity of CFC surviving 1000 rads was undiminished as judged by their ability to form large colonies. Estimates of CFC kill by hydroxyurea were also affected by the level of CSA.", "contents": "Relationship of colony-stimulating activity to apparent kill of human colony-forming cells by irradiation and hydroxyurea. Suspensions of human bone marrow cells were subjected to 137Cs irradiation in vitro and then cultured in semisolid agar medium. Cultures of irradiated cells were stimulated with colony-stimulating activity (CSA) of different potencies, and it was found that the amount of stimulation applied to cultures influenced the apparent kill of colony-forming cells (CFC). It was also found that the effects of irradiation on colony formation were not confined to CFC kill since medium conditioned by cells during irradiation exhibited stimulatory and inhibitory properties after treatment by 600 and 1000 rads, respectively. Studies in which irradiated cells were pretreated with hydroxyurea indicated that CFC in the DNA synthetic phase of the cell cycle were particularly sensitive to low doses of irradiation. The proliferative capacity of CFC surviving 1000 rads was undiminished as judged by their ability to form large colonies. Estimates of CFC kill by hydroxyurea were also affected by the level of CSA."}
{"id": "PMID:1082782", "title": "Human bone marrow lymphocytes: B and T cell precursors and subpopulations.", "content": "Characterization of the different lymphocyte populations in normal human bone marrow (BM) was attempted and compared to that in the peripheral blood (PB). B cells comprised 34% +/- 11% of lymphocytes in BM and 23% +/- 9% in PB. The majority of B cells carried IgM in BM and IgG in the PB. In the BM, cells carrying complement or Fc receptors were fewer than cells carrying Ig, but in the PB they were equal. T cells comprised 6% +/- 4% of lymphocytes in the BM and 62% +/- 7% in the PB. The majority of BM lymphocytes did not have B or T cell markers; these probably included B and T cell precursors. BM lymphocytes carrying surface Ig increased in a 7-day culture, whereas those of the PB decreased. Pokeweed mitogen induced Ig synthesis in B cells of PB but not those of BM. BM-T cells were more efficient than PB-T cells in inhibiting Ig synthesis of PB-B cells. These results indicate that the BM compartment contains immature B cells that are capable of partial differentiation and maturation in vitro. BM-B lymphocytes are probably not involved in the effector phase of the immune response since they are unable to synthesize Ig and because they carry few receptors for complement of Fc, BM-T lymphocytes are very few and have suppressor capability and therefore may play an essential role in regulation of Ig synthesis by B cells.", "contents": "Human bone marrow lymphocytes: B and T cell precursors and subpopulations. Characterization of the different lymphocyte populations in normal human bone marrow (BM) was attempted and compared to that in the peripheral blood (PB). B cells comprised 34% +/- 11% of lymphocytes in BM and 23% +/- 9% in PB. The majority of B cells carried IgM in BM and IgG in the PB. In the BM, cells carrying complement or Fc receptors were fewer than cells carrying Ig, but in the PB they were equal. T cells comprised 6% +/- 4% of lymphocytes in the BM and 62% +/- 7% in the PB. The majority of BM lymphocytes did not have B or T cell markers; these probably included B and T cell precursors. BM lymphocytes carrying surface Ig increased in a 7-day culture, whereas those of the PB decreased. Pokeweed mitogen induced Ig synthesis in B cells of PB but not those of BM. BM-T cells were more efficient than PB-T cells in inhibiting Ig synthesis of PB-B cells. These results indicate that the BM compartment contains immature B cells that are capable of partial differentiation and maturation in vitro. BM-B lymphocytes are probably not involved in the effector phase of the immune response since they are unable to synthesize Ig and because they carry few receptors for complement of Fc, BM-T lymphocytes are very few and have suppressor capability and therefore may play an essential role in regulation of Ig synthesis by B cells."}
{"id": "PMID:1082783", "title": "Effect of atropine on the decay of miniature end-plate currents at the frog neuromuscular junction.", "content": "1 The effect of atropine on the time constant of decay (tau) of miniature end-plate currents was investigated. 2. The atropine-induced shortening of tau was accompanied by a reduction in the voltage-sensitivity of tau. 3. This effect of atropine was reversible and dose-dependent, the voltage sensitivity being abolished with high concentrations of atropine (2.5 X 10(-5)M).", "contents": "Effect of atropine on the decay of miniature end-plate currents at the frog neuromuscular junction. 1 The effect of atropine on the time constant of decay (tau) of miniature end-plate currents was investigated. 2. The atropine-induced shortening of tau was accompanied by a reduction in the voltage-sensitivity of tau. 3. This effect of atropine was reversible and dose-dependent, the voltage sensitivity being abolished with high concentrations of atropine (2.5 X 10(-5)M)."}
{"id": "PMID:1082784", "title": "Early experience with the Boerema button for bleeding oesophageal varices.", "content": "Early experience with the use of the Boerema button for bleeding oesophageal varices is reported. A simplified technique for its insertion is described, and of the 6 patients so treated, only 1 died. No patient has re-bled in the short follow-up period available, and oesophageal appearances suggest that prolonged relief from bleeding can be anticipated. Further exploration of the button ligation transection technique is suggested not only for control of acute bleeding, but also for patients unsuitable for shunt surgery.", "contents": "Early experience with the Boerema button for bleeding oesophageal varices. Early experience with the use of the Boerema button for bleeding oesophageal varices is reported. A simplified technique for its insertion is described, and of the 6 patients so treated, only 1 died. No patient has re-bled in the short follow-up period available, and oesophageal appearances suggest that prolonged relief from bleeding can be anticipated. Further exploration of the button ligation transection technique is suggested not only for control of acute bleeding, but also for patients unsuitable for shunt surgery."}
{"id": "PMID:1082786", "title": "The morphology of motoneurons and dorsal root fibers in the frog's spinal cord.", "content": "Ventral and dorsal roots of the frog's spinal cord were filled with cobaltous chloride, and the resulting cobaltous sulfide precipitate, following treatment with H2S-buffer solutions, was intensified with physical developers. A ventromedial and a dorsolateral motoneuron group could be discerned in the ventral horn. The ventromedial, motoneurons gave origin to a strong dendrite crossing to the contralateral side. In the dendritic arborization pattern of the dorsolateral motoneurons a dorsomedial, a dorsal and a lateral dendritic array were distinguished. They were regarded as representing three different input channels to the motoneurons. Intramedullary branching of motor axons and recurrent axon collaterals were never observed. The dorsal root could be divided into a medial and lateral division carrying small and large caliber fibers, respectively. The end-branches of the small caliber fibers were seen to terminate in the substantia gelatinosa. Fine collaterals of the large caliber fibers also terminated in the substantia gelatinosa; coarser collaterals penetrated deeper and terminated in a triangular-shaped area in the base of the dorsal horn and in the intermediate gray matter. From this area a tail was followed into the ventral horn and several synapses were seen on the proximal dendrites and on the somata of motoneurons. A few dorsal root fibers could be seen crossing to the contralateral side.", "contents": "The morphology of motoneurons and dorsal root fibers in the frog's spinal cord. Ventral and dorsal roots of the frog's spinal cord were filled with cobaltous chloride, and the resulting cobaltous sulfide precipitate, following treatment with H2S-buffer solutions, was intensified with physical developers. A ventromedial and a dorsolateral motoneuron group could be discerned in the ventral horn. The ventromedial, motoneurons gave origin to a strong dendrite crossing to the contralateral side. In the dendritic arborization pattern of the dorsolateral motoneurons a dorsomedial, a dorsal and a lateral dendritic array were distinguished. They were regarded as representing three different input channels to the motoneurons. Intramedullary branching of motor axons and recurrent axon collaterals were never observed. The dorsal root could be divided into a medial and lateral division carrying small and large caliber fibers, respectively. The end-branches of the small caliber fibers were seen to terminate in the substantia gelatinosa. Fine collaterals of the large caliber fibers also terminated in the substantia gelatinosa; coarser collaterals penetrated deeper and terminated in a triangular-shaped area in the base of the dorsal horn and in the intermediate gray matter. From this area a tail was followed into the ventral horn and several synapses were seen on the proximal dendrites and on the somata of motoneurons. A few dorsal root fibers could be seen crossing to the contralateral side."}
{"id": "PMID:1082791", "title": "Five years' experience with aortocoronary bypass grafting.", "content": "During a 5-year period (Apr. 14, 1970 to Apr. 14, 1975) 930 patients underwent aortocoronary bypass grafting; the procedure was done as an emergency in 141. Of the entire group 3.3% died at operation, 1.6% died in hospital and 5.8% died later; of the patients undergoing emergency grafting 12.1% died at operation and 5.7% died later. From a detailed analysis of the first 600 patients it was found that both operative and late mortality were clearly related to two factors: severe left ventricular dysfunction at the time of operation and inadequate surgical treatment because of insertion of insufficient numbers of grafts or because of poor blood flow through the grafts.", "contents": "Five years' experience with aortocoronary bypass grafting. During a 5-year period (Apr. 14, 1970 to Apr. 14, 1975) 930 patients underwent aortocoronary bypass grafting; the procedure was done as an emergency in 141. Of the entire group 3.3% died at operation, 1.6% died in hospital and 5.8% died later; of the patients undergoing emergency grafting 12.1% died at operation and 5.7% died later. From a detailed analysis of the first 600 patients it was found that both operative and late mortality were clearly related to two factors: severe left ventricular dysfunction at the time of operation and inadequate surgical treatment because of insertion of insufficient numbers of grafts or because of poor blood flow through the grafts."}
{"id": "PMID:1082794", "title": "An approach to immunotherapy using antibody to IgE in mast cell leukemia.", "content": "Passive immunotherapy was attempted in a patient with mast cell leukemia using antibody against IgE. The results of both in vitro and in vivo studies indicate that although receptor sites for IgE were retained by the malignant mast cell, a secretory defect was present characterized by the spontaneous release of histamine and an impaired secretory response to anti-IgE antibody. Anti-IgE antibody selectively and reproducibly reduced the number of circulating mast cells probably by facilitating their permanent uptake by the reticuloendothelial system. Tolerance was not achieved with high dose deaggregated sheep IgG, nor were we able to confirm the effectivity of immunochemotherapy based on linking chlorambucil to antibody directed against the tumor-associated \"antigen\" IgE.", "contents": "An approach to immunotherapy using antibody to IgE in mast cell leukemia. Passive immunotherapy was attempted in a patient with mast cell leukemia using antibody against IgE. The results of both in vitro and in vivo studies indicate that although receptor sites for IgE were retained by the malignant mast cell, a secretory defect was present characterized by the spontaneous release of histamine and an impaired secretory response to anti-IgE antibody. Anti-IgE antibody selectively and reproducibly reduced the number of circulating mast cells probably by facilitating their permanent uptake by the reticuloendothelial system. Tolerance was not achieved with high dose deaggregated sheep IgG, nor were we able to confirm the effectivity of immunochemotherapy based on linking chlorambucil to antibody directed against the tumor-associated \"antigen\" IgE."}
{"id": "PMID:1082795", "title": "Lymphocyte abnormalities in untreated patients with Hodgkin's disease.", "content": "The study comprises 38 unselected and untreated patients with Hodgkin's disease (HD) and 23 healthy persons. Highly purified blood lymphocytes were analyzed for cells forming rosettes with sheep red blood cells (T lymphocytes), and lymphocytes bearing surface immunoglobulin (B lymphocytes) and/or carrying receptors for complement. Their DNA synthesis, spontaneously, or after activation with mitogens (phytohemagglutinin, concanavallin A, poke weed mitogen) and purified protein derivative (PPD) was measured. Delayed skin hypersensitivity to PPD and mumps antigen was studied. Most HD patients had low numbers of T lymphocytes (50% of the cases below normal range) while the mean B-lymphocyte level was normal but with a greater variation than in the control group. Lymphocytes from most patients were poorly stimulated by T-cell mitogens. Two-thirds of the patients and one healthy control had negative skin reaction to 2 TU PPD and the DNA synthesis of their lymphocytes after activation with PPD was low. Large lymphoid cells (greater than 9-mm diameter) were commonly present in HD blood and the spontaneous DNA synthesis was high, particularly in lymphocytes from stage B patients. The percentage of T lymphocytes and the stimulation of lymphocytes by T-cell mitogens or by PPD, a T-lymphocyte function, did not correlate and each test only detected defects in about half the cases. Simultaneous application of all tests revealed abnormalities of blood T lymphocytes in 33 out of 38 patients. Although the defects were usually more pronounced in patients with advanced disease, the impairment of T lymphocytes and their functions is present in all stages of Hodgkin's disease.", "contents": "Lymphocyte abnormalities in untreated patients with Hodgkin's disease. The study comprises 38 unselected and untreated patients with Hodgkin's disease (HD) and 23 healthy persons. Highly purified blood lymphocytes were analyzed for cells forming rosettes with sheep red blood cells (T lymphocytes), and lymphocytes bearing surface immunoglobulin (B lymphocytes) and/or carrying receptors for complement. Their DNA synthesis, spontaneously, or after activation with mitogens (phytohemagglutinin, concanavallin A, poke weed mitogen) and purified protein derivative (PPD) was measured. Delayed skin hypersensitivity to PPD and mumps antigen was studied. Most HD patients had low numbers of T lymphocytes (50% of the cases below normal range) while the mean B-lymphocyte level was normal but with a greater variation than in the control group. Lymphocytes from most patients were poorly stimulated by T-cell mitogens. Two-thirds of the patients and one healthy control had negative skin reaction to 2 TU PPD and the DNA synthesis of their lymphocytes after activation with PPD was low. Large lymphoid cells (greater than 9-mm diameter) were commonly present in HD blood and the spontaneous DNA synthesis was high, particularly in lymphocytes from stage B patients. The percentage of T lymphocytes and the stimulation of lymphocytes by T-cell mitogens or by PPD, a T-lymphocyte function, did not correlate and each test only detected defects in about half the cases. Simultaneous application of all tests revealed abnormalities of blood T lymphocytes in 33 out of 38 patients. Although the defects were usually more pronounced in patients with advanced disease, the impairment of T lymphocytes and their functions is present in all stages of Hodgkin's disease."}
{"id": "PMID:1082796", "title": "Treatment of spontaneous leukemia in AKR mice with chemotherapy, immunotherapy, or interferon.", "content": "AKR mice are genetically destined to develop Gross (RNA) virus-induced lymphatic leukemia. Leukemic AKR mice treated with combination vincristine, cyclophosphamide (Cytoxan), and 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea sustained a 180% increase of life-span. Combination chemotherapy plus immunization with neuraminidase-treated allogeneic (Gross virus-induced) G2G leukemic cells intradermally resulted in 35% of animals surviving beyond 150 days without evidence of the disease. It is significant that allogeneic E2G leukemic cells as immunogen were as effective in prolonging the life-span of the immunized leukemic AKR mice as were syngeneic leukemic thymocytes. Virazole (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), an antiviral compound, alone showed no apparent antitumor effect. However, in experiments in which the clinically diagnosed leukemic AKR mice received a combination of cytoreductive therapy [vincristine plus prednisone or, more effectively, vincristine, Cytoxan plus 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea, followed by Virazole], there was a noticeable reduction of the viral titer, a delay in the reappearance of viable clonogenic cells, and an increase in the survival time for the leukemic AKR mice as compared to those receiving cytoreductive therapy alone. The effectiveness of purified mouse interferon in AKR mice was also examined. The decrease in the viral titer of animals that received interferon treatment was markedly greater than of those receiving a combination of cytoreductive therapy with Virazole or immunotherapy. The administration of mouse interferon had a direct effect on the appearance of the spontaneous leukemia in AKR mice. The median life-span of the control animals was 36 weeks, whereas 45% of the AKR mice treated with five doses of 5 X 10(4) units of interferon are still alive at 54 weeks of age. Thus, interferon not only reduces the Gross murine leukemia virus titer in the chronically infected AKR mice but also significantly delays the appearance of the primary lymphoma.", "contents": "Treatment of spontaneous leukemia in AKR mice with chemotherapy, immunotherapy, or interferon. AKR mice are genetically destined to develop Gross (RNA) virus-induced lymphatic leukemia. Leukemic AKR mice treated with combination vincristine, cyclophosphamide (Cytoxan), and 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea sustained a 180% increase of life-span. Combination chemotherapy plus immunization with neuraminidase-treated allogeneic (Gross virus-induced) G2G leukemic cells intradermally resulted in 35% of animals surviving beyond 150 days without evidence of the disease. It is significant that allogeneic E2G leukemic cells as immunogen were as effective in prolonging the life-span of the immunized leukemic AKR mice as were syngeneic leukemic thymocytes. Virazole (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), an antiviral compound, alone showed no apparent antitumor effect. However, in experiments in which the clinically diagnosed leukemic AKR mice received a combination of cytoreductive therapy [vincristine plus prednisone or, more effectively, vincristine, Cytoxan plus 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea, followed by Virazole], there was a noticeable reduction of the viral titer, a delay in the reappearance of viable clonogenic cells, and an increase in the survival time for the leukemic AKR mice as compared to those receiving cytoreductive therapy alone. The effectiveness of purified mouse interferon in AKR mice was also examined. The decrease in the viral titer of animals that received interferon treatment was markedly greater than of those receiving a combination of cytoreductive therapy with Virazole or immunotherapy. The administration of mouse interferon had a direct effect on the appearance of the spontaneous leukemia in AKR mice. The median life-span of the control animals was 36 weeks, whereas 45% of the AKR mice treated with five doses of 5 X 10(4) units of interferon are still alive at 54 weeks of age. Thus, interferon not only reduces the Gross murine leukemia virus titer in the chronically infected AKR mice but also significantly delays the appearance of the primary lymphoma."}
{"id": "PMID:1082798", "title": "The differentiation of cytotoxic T lymphocytes in vitro: an ultrastructural study.", "content": "T killer cells were identified morphologically in two different culture systems. The first system allowed identification by comparing under identical culture conditions two types of stimulation, one leading to cytotoxic activity levels that were very high (H-2 stimulation) and the other to levels which were practically nil (M-locus stimulation). A second system allowed identification by the use of a collaborative system in which relatively pure precursors of T killer cells were mixed with precursors of T amplifier cells from which they were distinguishable by their Thy-l antigen. In this latter case, surface labelling techniques had to be used. As in the in vivo situation, T killer cells were medium-sized pale cells with evidence of a well-developed secretory and motile apparatus. No evidence of highly active protein synthesis was found. Other T cells were dark and mainly derived from the T amplifier population, again consistent with in vivo findings. There was considerable background proliferation, even in the absence of any added antigen, and this included the formation of some well-developed plasma cells.", "contents": "The differentiation of cytotoxic T lymphocytes in vitro: an ultrastructural study. T killer cells were identified morphologically in two different culture systems. The first system allowed identification by comparing under identical culture conditions two types of stimulation, one leading to cytotoxic activity levels that were very high (H-2 stimulation) and the other to levels which were practically nil (M-locus stimulation). A second system allowed identification by the use of a collaborative system in which relatively pure precursors of T killer cells were mixed with precursors of T amplifier cells from which they were distinguishable by their Thy-l antigen. In this latter case, surface labelling techniques had to be used. As in the in vivo situation, T killer cells were medium-sized pale cells with evidence of a well-developed secretory and motile apparatus. No evidence of highly active protein synthesis was found. Other T cells were dark and mainly derived from the T amplifier population, again consistent with in vivo findings. There was considerable background proliferation, even in the absence of any added antigen, and this included the formation of some well-developed plasma cells."}
{"id": "PMID:1082802", "title": "Correlation of platelet survival time with occlusion of saphenous vein aorto-coronary bypass grafts.", "content": "Platelet survival time is frequently shortened in patients with coronary artery disease, and it is one of several factors that might contribute to graft occlusion after saphenous vein coronary artery bypass (CAB). In 35 patients with CAB, average platelet survival (autologous labeling with 51Chromium) was shortened in 20 with one or more saphenous vein grafts occluded and normal in 15 with all grafts open. Of 15 with all grafts open, individual levels of platelet survival were normal in 10 while in 20 with one or more grafts occluded platelet survival was normal in only one. Platelet survival was not altered by coronary surgery and nine of ten with shortened platelet survival pre-operatively had graft occlusion. Platelet survival did not correlate with either parent artery occlusion or serum lipoproteins. These findings suggest a relationship between shortened platelet survival and saphenous vein graft occlusion and suggest that platelet suppressant therapy might be useful in preventing graft occlusion.", "contents": "Correlation of platelet survival time with occlusion of saphenous vein aorto-coronary bypass grafts. Platelet survival time is frequently shortened in patients with coronary artery disease, and it is one of several factors that might contribute to graft occlusion after saphenous vein coronary artery bypass (CAB). In 35 patients with CAB, average platelet survival (autologous labeling with 51Chromium) was shortened in 20 with one or more saphenous vein grafts occluded and normal in 15 with all grafts open. Of 15 with all grafts open, individual levels of platelet survival were normal in 10 while in 20 with one or more grafts occluded platelet survival was normal in only one. Platelet survival was not altered by coronary surgery and nine of ten with shortened platelet survival pre-operatively had graft occlusion. Platelet survival did not correlate with either parent artery occlusion or serum lipoproteins. These findings suggest a relationship between shortened platelet survival and saphenous vein graft occlusion and suggest that platelet suppressant therapy might be useful in preventing graft occlusion."}
{"id": "PMID:1082804", "title": "Synthesis of folate polyglutamates in human cells.", "content": "1. Synthesis of polyglutamate derivatives from radioactively labelled folic acid, folinic acid and methotrexate has been studied in human phytohaemagglutinin-transformed lymphocytes. 2. With labelled folic acid as the precursor, approximately 20% of the labelled cell folate was found in each of the mono-, tetra-, penta- and hexaglutamate peaks after 72 h of incubation. At earlier times, the amounts of labelled folate polyglutamates were proportionately greater and the amount of labelled higher folate polyglutamates was lower. After only 4 h incubation over 80% of the intracellular labelled folate was still in the monoglutamate form. 3. With labelled folinic acid used as precursor, approximately 30% of labelled cell folate after 72 h incubation was in the form of folate pentaglutamate, with tri- and tetra-glutamate forming the other major peaks. The speed of formation of polyglutamate derivatives was substantially more rapid than from folic acid. 4. Methotrexate was found to decrease considerably the amount of folate polyglutamate formation from labelled folic acid in lymphocytes byt nevertheless some di-, tri- and traces of tetra-glutamate were formed. On the other hand, methotrexate had no effect on folate polyglutamate formation with labelled folinic acid used as the precusor. 5. Polyglutamate derivatives of labelled methotrexate were formed by human lymphocytes with mean amounts of 4-5% of di-, 1-5% of tri-, 1-0% of tetra- and 0-7% of penta-glutamate derivatives being formed over the period 48-72h of culture. 6. Pentaglutamate derivatives probably constitute the largest group of intracellular folates in human cells but a complex mixture exists. 7. The enzyme synthesizing polyglutamate derivatives of folate in human cells prefers a reduced folate as substrate but the requirement for a reduced form is not absolute. The nature of the reduced folate is uncertain but it is suggested on the basis of previous work that tetrahydrofolate rather than methylhydropfolate or any other reduced folate monoglutamate is the preferred substrate.", "contents": "Synthesis of folate polyglutamates in human cells. 1. Synthesis of polyglutamate derivatives from radioactively labelled folic acid, folinic acid and methotrexate has been studied in human phytohaemagglutinin-transformed lymphocytes. 2. With labelled folic acid as the precursor, approximately 20% of the labelled cell folate was found in each of the mono-, tetra-, penta- and hexaglutamate peaks after 72 h of incubation. At earlier times, the amounts of labelled folate polyglutamates were proportionately greater and the amount of labelled higher folate polyglutamates was lower. After only 4 h incubation over 80% of the intracellular labelled folate was still in the monoglutamate form. 3. With labelled folinic acid used as precursor, approximately 30% of labelled cell folate after 72 h incubation was in the form of folate pentaglutamate, with tri- and tetra-glutamate forming the other major peaks. The speed of formation of polyglutamate derivatives was substantially more rapid than from folic acid. 4. Methotrexate was found to decrease considerably the amount of folate polyglutamate formation from labelled folic acid in lymphocytes byt nevertheless some di-, tri- and traces of tetra-glutamate were formed. On the other hand, methotrexate had no effect on folate polyglutamate formation with labelled folinic acid used as the precusor. 5. Polyglutamate derivatives of labelled methotrexate were formed by human lymphocytes with mean amounts of 4-5% of di-, 1-5% of tri-, 1-0% of tetra- and 0-7% of penta-glutamate derivatives being formed over the period 48-72h of culture. 6. Pentaglutamate derivatives probably constitute the largest group of intracellular folates in human cells but a complex mixture exists. 7. The enzyme synthesizing polyglutamate derivatives of folate in human cells prefers a reduced folate as substrate but the requirement for a reduced form is not absolute. The nature of the reduced folate is uncertain but it is suggested on the basis of previous work that tetrahydrofolate rather than methylhydropfolate or any other reduced folate monoglutamate is the preferred substrate."}
{"id": "PMID:1082810", "title": "[Early diagnosis of leukaemic transformation of malignant non-hodgkin lymphoma in childhood (author's transl)].", "content": "Fresh tumour cells from four children, aged 3-11 years, with malignant non-Hodgkin lymphoma were examined with four different immunological cell markers (E-rosettes, membrane-immunoglobulins, receptors for complement, and receptors for Fc fragment). In three patients a T-cell marker (E-rosettes) was found in most of their tumour cells, the marker differing from that of T-lymphcoytes by different temperature kinetics. This difference may be used to recognise earlier than previously possible the frequently observed diffuse infiltration of bone marrow (leukaemic transformation) and of the nervous system in the course of the disease.", "contents": "[Early diagnosis of leukaemic transformation of malignant non-hodgkin lymphoma in childhood (author's transl)]. Fresh tumour cells from four children, aged 3-11 years, with malignant non-Hodgkin lymphoma were examined with four different immunological cell markers (E-rosettes, membrane-immunoglobulins, receptors for complement, and receptors for Fc fragment). In three patients a T-cell marker (E-rosettes) was found in most of their tumour cells, the marker differing from that of T-lymphcoytes by different temperature kinetics. This difference may be used to recognise earlier than previously possible the frequently observed diffuse infiltration of bone marrow (leukaemic transformation) and of the nervous system in the course of the disease."}
{"id": "PMID:1082811", "title": "[Pain suppression in the trigeminal region by means of transcutaneous nerve stimulation].", "content": "On the basis of the gate control theory, an attempt was made to influence acute pain by transcutaneous nerve stimulation (TNS) in the region of the trigeminal nerve. After favorable first results in clinical application, we determined sensation and pain treshold values of teeth in two groups of patients by exactly defined electric stimuli of different degrees. After transcutaneous electro-stimulation of the pertinent ramus of the trigeminal nerve with rectangular stimulating currents, the threshold measurements were repeated. The thresholds were raised by an average 79% as far as sensation was concerned, and by an average 69% as far as pain was concerned.", "contents": "[Pain suppression in the trigeminal region by means of transcutaneous nerve stimulation]. On the basis of the gate control theory, an attempt was made to influence acute pain by transcutaneous nerve stimulation (TNS) in the region of the trigeminal nerve. After favorable first results in clinical application, we determined sensation and pain treshold values of teeth in two groups of patients by exactly defined electric stimuli of different degrees. After transcutaneous electro-stimulation of the pertinent ramus of the trigeminal nerve with rectangular stimulating currents, the threshold measurements were repeated. The thresholds were raised by an average 79% as far as sensation was concerned, and by an average 69% as far as pain was concerned."}
{"id": "PMID:1082812", "title": "[Hypothetical concept: the physiopathological entity of monoaminergic psychoses].", "content": "The importance of amino acid transport across the blood-brain barrier as the limiting factor in the metabolism of monoamines has been emphasized by many recent publications. Particularly critical is the transport of tryptophan, since tryptophan hydroxylase is not saturated. This transport is regulated by complex mechanisms, both at the periphery (total and free plasmatic levels and levels of the other essential amino acids) and centraly (by feedback mechanism initiated at the pre- and post-synaptic levels). The hydroxylated derivatives of tryptophan and tyrosine, i.e. 5-HTP and L-DOPA, most probably share the same transport mechanism as these amino acids themselves. In manic-depressive patients, the uptake of L-5-HTP is increased during the depressive phase, while the uptake of L-DOPA, is increased during the manic phase. We suggest that an increase in the uptake of tryptophan may set off oscillations in all the monoaminergic systems, thus providing a biochemical model of manic-depressive psychosis. In terms of this model, melancholy would be due to a hyperserotoninergic syndrome together with a relative hypocatecholaminergic syndrome. Mania would be due to a homogeneous hypercatecholaminergic syndrome together with a relative hyposerotoninergic syndrome. Such a model is compatible with present knowledge of the physiology of monoamines, of the semeiology and biological disturbances of manic-depressive psychosis, and of the treatment of this disease. It reconciles the monoaminergic and ionic theories of the disease better than other existing hypotheses. A reduced transport of tryptophan with a secondary increase in the transport ot tyrosine provides a conceivable model for schizophrenia. Indeed, a serotoninergic hypoactivity coupled with a dopaminergic hyperactivity, with or without a noradrenergic deficiency, would account for the semeiology quite adequately. This model too would be compatible with present knowledge of monoamine physiology, of the biochemical disturbances underlying schizophrenia and of the mode of action of anti-psychotic drugs. This unitarian heuristic concept of the monoaminergic psychoses would be in better agreement with the classic clinical data concerning this disease (typology intermediate syndromes and crossed heredity).", "contents": "[Hypothetical concept: the physiopathological entity of monoaminergic psychoses]. The importance of amino acid transport across the blood-brain barrier as the limiting factor in the metabolism of monoamines has been emphasized by many recent publications. Particularly critical is the transport of tryptophan, since tryptophan hydroxylase is not saturated. This transport is regulated by complex mechanisms, both at the periphery (total and free plasmatic levels and levels of the other essential amino acids) and centraly (by feedback mechanism initiated at the pre- and post-synaptic levels). The hydroxylated derivatives of tryptophan and tyrosine, i.e. 5-HTP and L-DOPA, most probably share the same transport mechanism as these amino acids themselves. In manic-depressive patients, the uptake of L-5-HTP is increased during the depressive phase, while the uptake of L-DOPA, is increased during the manic phase. We suggest that an increase in the uptake of tryptophan may set off oscillations in all the monoaminergic systems, thus providing a biochemical model of manic-depressive psychosis. In terms of this model, melancholy would be due to a hyperserotoninergic syndrome together with a relative hypocatecholaminergic syndrome. Mania would be due to a homogeneous hypercatecholaminergic syndrome together with a relative hyposerotoninergic syndrome. Such a model is compatible with present knowledge of the physiology of monoamines, of the semeiology and biological disturbances of manic-depressive psychosis, and of the treatment of this disease. It reconciles the monoaminergic and ionic theories of the disease better than other existing hypotheses. A reduced transport of tryptophan with a secondary increase in the transport ot tyrosine provides a conceivable model for schizophrenia. Indeed, a serotoninergic hypoactivity coupled with a dopaminergic hyperactivity, with or without a noradrenergic deficiency, would account for the semeiology quite adequately. This model too would be compatible with present knowledge of monoamine physiology, of the biochemical disturbances underlying schizophrenia and of the mode of action of anti-psychotic drugs. This unitarian heuristic concept of the monoaminergic psychoses would be in better agreement with the classic clinical data concerning this disease (typology intermediate syndromes and crossed heredity)."}
{"id": "PMID:1082813", "title": "Deoxyribosyl transfer catalysis with trans-N-deoxyribosylase. Kinetic study of purine(pyrimidine) to pyrimidine(purine) trans-N-deoxyribosylase.", "content": "Kinetic studies were carried out in order to investigate the enzymic mechanism of a 215-fold-purified purine(pyrimidine) nucleoside: purine(pyrimidine) deoxyribosyl transferase fraction from Lactobacillus helveticus. A variety of natural deoxyribonucleosides and bases were used as substrates. Initial velocity, product inhibition and isotopic exchange studies are consistent with a ping-pong bi-bi mechanism. The kinetic parameters are used to show that this fraction is free from any contamination by a specific purine nucleoside: purine deoxyribosyl transferase also found in the same strain of L. helveticus.", "contents": "Deoxyribosyl transfer catalysis with trans-N-deoxyribosylase. Kinetic study of purine(pyrimidine) to pyrimidine(purine) trans-N-deoxyribosylase. Kinetic studies were carried out in order to investigate the enzymic mechanism of a 215-fold-purified purine(pyrimidine) nucleoside: purine(pyrimidine) deoxyribosyl transferase fraction from Lactobacillus helveticus. A variety of natural deoxyribonucleosides and bases were used as substrates. Initial velocity, product inhibition and isotopic exchange studies are consistent with a ping-pong bi-bi mechanism. The kinetic parameters are used to show that this fraction is free from any contamination by a specific purine nucleoside: purine deoxyribosyl transferase also found in the same strain of L. helveticus."}
{"id": "PMID:1082815", "title": "Control mechanisms of endotoxin and particulate material stimulation of hemopoietic colony forming cell differentiation.", "content": "Through injection of particulate materials and endotoxins (ET), possible control mechanisms of murine hemopoietic colony forming cells (CFC) were investigated. The experimental system employed two inbred strains of mice that responded with a similar increase in splenic CFC after injection of particulate materials, sheep red blood cells (SRBC) and colloidal carbon (cc), but differed in their response to ET. The strains similarly phagocytosed radiolabelled SRBC and ET, suggesting that the CFC changes were not dependent solely on phagocytosis of ET. The CFC from low ET responder strain responded in vitro to colony stimulating factor (CSF) elicited by ET in serum of the high responder strain, demonstrating that lack of response to ET is due, at least in part, to failure to produce CSF. Subjection of serum from either strain to chloroform extraction did not significantly increase its stimulating capacity, suggesting that the increased stimulatory ability in response to ET was due to increase in the CSF level rather than to a decrease in the level of inhibition.", "contents": "Control mechanisms of endotoxin and particulate material stimulation of hemopoietic colony forming cell differentiation. Through injection of particulate materials and endotoxins (ET), possible control mechanisms of murine hemopoietic colony forming cells (CFC) were investigated. The experimental system employed two inbred strains of mice that responded with a similar increase in splenic CFC after injection of particulate materials, sheep red blood cells (SRBC) and colloidal carbon (cc), but differed in their response to ET. The strains similarly phagocytosed radiolabelled SRBC and ET, suggesting that the CFC changes were not dependent solely on phagocytosis of ET. The CFC from low ET responder strain responded in vitro to colony stimulating factor (CSF) elicited by ET in serum of the high responder strain, demonstrating that lack of response to ET is due, at least in part, to failure to produce CSF. Subjection of serum from either strain to chloroform extraction did not significantly increase its stimulating capacity, suggesting that the increased stimulatory ability in response to ET was due to increase in the CSF level rather than to a decrease in the level of inhibition."}
{"id": "PMID:1082816", "title": "Assessment of bone marrow function: microscopy versus the agar colony assay.", "content": "Changes in bone marrow status followig cytotoxic chemotherapy were measured by means of peripheral blood counts, bone marrow cytology and colony-formation in agar culture. A correlation is described between colony-forming ability and marrow differential count.", "contents": "Assessment of bone marrow function: microscopy versus the agar colony assay. Changes in bone marrow status followig cytotoxic chemotherapy were measured by means of peripheral blood counts, bone marrow cytology and colony-formation in agar culture. A correlation is described between colony-forming ability and marrow differential count."}
{"id": "PMID:1082817", "title": "Anaemia and the induction of myeloid leukaemia in RFM mice.", "content": "The results from short term experiments provide little support for the idea that the anaemic stress produced by phlebotomy acts as a trigger for myeloid leukaemia in mice genetically prone to the development of the disease. Longer term investigation has also failed to reveal any difference between bled and control mice and at this time (16 months after bleeding) no animals have died from myeloid leukaemia. These observations are in contrast to the recently reported effect of phlebotomy as a trigger for myeloid leukaemia in unirradiated RFM mice and in irradiated rats. The results given in this paper are discussed in relation to the haemopoietic stem cell compartment in leukaemogenesis and the relationship between these cells and the mature cells in the peripheral blood.", "contents": "Anaemia and the induction of myeloid leukaemia in RFM mice. The results from short term experiments provide little support for the idea that the anaemic stress produced by phlebotomy acts as a trigger for myeloid leukaemia in mice genetically prone to the development of the disease. Longer term investigation has also failed to reveal any difference between bled and control mice and at this time (16 months after bleeding) no animals have died from myeloid leukaemia. These observations are in contrast to the recently reported effect of phlebotomy as a trigger for myeloid leukaemia in unirradiated RFM mice and in irradiated rats. The results given in this paper are discussed in relation to the haemopoietic stem cell compartment in leukaemogenesis and the relationship between these cells and the mature cells in the peripheral blood."}
{"id": "PMID:1082819", "title": "Behavior of horizontal semicircular canal afferents in alert monkey during vestibular and optokinetic stimulation.", "content": "The behavior of single vestibular nerve fibers from the lateral semicircular canal was recorded during sinusoidal oscillations of the head, during optokinetic stimulation with the head stationary, and during spontaneous oculomotor behavior in the alert monkey. The response of similar fibers to adequate vestibular stimulation was also studied in some of the animals under deeply anesthetized conditions. In the alert animals all units were spontaneously active and their discharge was modulated only by adequate vestibular stimulation. Ipsilateral horizontal rotations of the head were excitatory for all units. No modification of this basic vestibular response by visual stimulation including full-field striped drum rotation was observed. Furthermore no correlation of unit activity with oculomotor function including voluntary saccadic and pursuit eye movements was found in any of the units. The regularity of spontaneous discharge was the most consistent characteristic that differentiated the unit response into types. Most units were very regular in discharge, but a few were very irregular. The averaging of unit discharge over several cycles of oscillatory head rotation showed that the irregular type units were also consistently modulated by adequate vestibular stimulation. Both regular and irregular type units were found in the anesthetized animals. Unimodal distributions of the quantitative values for unit resting discharge rate, sensitivity, and phase relationship were found. The distributions for these three parameters were similar in the units recorded in the anesthetized animals. Thus at least these characteristics of semicircular canal response seem not to be affected by the vestibular efferent system which should be altered or eliminated in the case of the anesthetized animals.", "contents": "Behavior of horizontal semicircular canal afferents in alert monkey during vestibular and optokinetic stimulation. The behavior of single vestibular nerve fibers from the lateral semicircular canal was recorded during sinusoidal oscillations of the head, during optokinetic stimulation with the head stationary, and during spontaneous oculomotor behavior in the alert monkey. The response of similar fibers to adequate vestibular stimulation was also studied in some of the animals under deeply anesthetized conditions. In the alert animals all units were spontaneously active and their discharge was modulated only by adequate vestibular stimulation. Ipsilateral horizontal rotations of the head were excitatory for all units. No modification of this basic vestibular response by visual stimulation including full-field striped drum rotation was observed. Furthermore no correlation of unit activity with oculomotor function including voluntary saccadic and pursuit eye movements was found in any of the units. The regularity of spontaneous discharge was the most consistent characteristic that differentiated the unit response into types. Most units were very regular in discharge, but a few were very irregular. The averaging of unit discharge over several cycles of oscillatory head rotation showed that the irregular type units were also consistently modulated by adequate vestibular stimulation. Both regular and irregular type units were found in the anesthetized animals. Unimodal distributions of the quantitative values for unit resting discharge rate, sensitivity, and phase relationship were found. The distributions for these three parameters were similar in the units recorded in the anesthetized animals. Thus at least these characteristics of semicircular canal response seem not to be affected by the vestibular efferent system which should be altered or eliminated in the case of the anesthetized animals."}
{"id": "PMID:1082820", "title": "Genetic control of LDH isozymes in the Rana esculenta complex.", "content": "Studies of LDH isozymes in the European green frogs showed that the synthesis of the B subunits is controlled by 3 alleles at a single genetic locus. The genetic evidence supports the hypothesis that Rana esculenta is the hybrid of R. lessonae X R. ridibunda.", "contents": "Genetic control of LDH isozymes in the Rana esculenta complex. Studies of LDH isozymes in the European green frogs showed that the synthesis of the B subunits is controlled by 3 alleles at a single genetic locus. The genetic evidence supports the hypothesis that Rana esculenta is the hybrid of R. lessonae X R. ridibunda."}
{"id": "PMID:1082821", "title": "Effect of orotic acid on liver glycogen of different animal species.", "content": "The effect of orotic acid on the liver glycogen content in the mice, frogs and catfish was studied. It was observed that the orotic acid significantly increases the glycogen content in the liver of mice and catfish as it does in rats. On the other hand it causes a fall of the glycogen level in frogs in experiments made both in autumn and spring. This effect was modified by amino acids administered together with orotic acid.", "contents": "Effect of orotic acid on liver glycogen of different animal species. The effect of orotic acid on the liver glycogen content in the mice, frogs and catfish was studied. It was observed that the orotic acid significantly increases the glycogen content in the liver of mice and catfish as it does in rats. On the other hand it causes a fall of the glycogen level in frogs in experiments made both in autumn and spring. This effect was modified by amino acids administered together with orotic acid."}
{"id": "PMID:1082822", "title": "A population of human lymphocytes staining for esterases.", "content": "A population of lymphocytes is found to stain positively for esterases. The positively staining lymphocytes are more predominant among T than B lymphocytes and are significantly increased on stimulation with PHA. Treatment with cholinestrase inhibitors reduces their number significantly.", "contents": "A population of human lymphocytes staining for esterases. A population of lymphocytes is found to stain positively for esterases. The positively staining lymphocytes are more predominant among T than B lymphocytes and are significantly increased on stimulation with PHA. Treatment with cholinestrase inhibitors reduces their number significantly."}
{"id": "PMID:1082825", "title": "Peripheral T-lymphocytes in juvenile-onset diabetics (JOD) and in maternity-onset diabetics (MOD).", "content": "The percentage and absolute number per mm.3 of peripheral T-lymphocytes were determined in 11 juvenile-onset diabetics (JOD), in 21 maturity-onset diabetics (MOD), and in 18 normal subjects (NS). The percentage was significantly lower in JOD (38.1) than in MOD (57.2) and NS (56.5). The absolute T-lymphocytes number per mm.3 was significantly lower in JOD (833) than in NS (1,260); this was also true for JOD as against MOD (1,026), even if the difference was not statistically significant. No difference was found between MOD and NS, or between MOD on oral therapy and on insulin treatment. The decrease of peripheral T-lymphocytes in JOD was not related to associated illness or drugs. The data presented suggest the possibility of an altered cell-mediated immunity in juvenile-onset diabetics.", "contents": "Peripheral T-lymphocytes in juvenile-onset diabetics (JOD) and in maternity-onset diabetics (MOD). The percentage and absolute number per mm.3 of peripheral T-lymphocytes were determined in 11 juvenile-onset diabetics (JOD), in 21 maturity-onset diabetics (MOD), and in 18 normal subjects (NS). The percentage was significantly lower in JOD (38.1) than in MOD (57.2) and NS (56.5). The absolute T-lymphocytes number per mm.3 was significantly lower in JOD (833) than in NS (1,260); this was also true for JOD as against MOD (1,026), even if the difference was not statistically significant. No difference was found between MOD and NS, or between MOD on oral therapy and on insulin treatment. The decrease of peripheral T-lymphocytes in JOD was not related to associated illness or drugs. The data presented suggest the possibility of an altered cell-mediated immunity in juvenile-onset diabetics."}
{"id": "PMID:1082826", "title": "[Clinical coronarographic characteristics and pathogenetic mechanism of angina pectoris with s-t elevation (author's transl)].", "content": "The study of 46 patients with frequent anginal episodes characterized by S-T elevation (so called \"variant angina pectoris\") demonstrated that this type of electrocardiographic pattern does not characterize a homogeneous group of patients. In fact, while in some patients angina occurred only at rest, in others it occurred also on exercise. Sometimes ecgraphic alterations characterized by S-T depression were observed on the same leads which on other occasions had shown S-T elevation. The angiographic picture revealed: absence of significant coronary alterations in 10% of cases, stenosis greater than 75% in one main branch in 29%, in two branches in 39% and in three branches in 22% of cases. The hemodynamic monitoring carried out on 14 of these patients demonstrated that the ecgraphic modifications occur before the onset of the hemodynamic parameters which control myocardial O2 consumption. This suggests a primitive reduction of regional myocardial blood supply as a cause of the ischaemic episodes. The study of the regional myocardial perfusion with 201Tl technique in 6 patients confirmed this hypothesis. Coronary angiography carried out during an ischemic episode showed that the reduction of myocardial blood supply was caused by a spasm of a large coronary artery involving a long segment of the vessel, reversible by nitroglycerin administration. Aorto-coronary by-pass operation performed on 6 patients was followed by the disappearance of pain in two patients, even though the \"by-pass\" patency was angiographically proved in two patients.", "contents": "[Clinical coronarographic characteristics and pathogenetic mechanism of angina pectoris with s-t elevation (author's transl)]. The study of 46 patients with frequent anginal episodes characterized by S-T elevation (so called \"variant angina pectoris\") demonstrated that this type of electrocardiographic pattern does not characterize a homogeneous group of patients. In fact, while in some patients angina occurred only at rest, in others it occurred also on exercise. Sometimes ecgraphic alterations characterized by S-T depression were observed on the same leads which on other occasions had shown S-T elevation. The angiographic picture revealed: absence of significant coronary alterations in 10% of cases, stenosis greater than 75% in one main branch in 29%, in two branches in 39% and in three branches in 22% of cases. The hemodynamic monitoring carried out on 14 of these patients demonstrated that the ecgraphic modifications occur before the onset of the hemodynamic parameters which control myocardial O2 consumption. This suggests a primitive reduction of regional myocardial blood supply as a cause of the ischaemic episodes. The study of the regional myocardial perfusion with 201Tl technique in 6 patients confirmed this hypothesis. Coronary angiography carried out during an ischemic episode showed that the reduction of myocardial blood supply was caused by a spasm of a large coronary artery involving a long segment of the vessel, reversible by nitroglycerin administration. Aorto-coronary by-pass operation performed on 6 patients was followed by the disappearance of pain in two patients, even though the \"by-pass\" patency was angiographically proved in two patients."}
{"id": "PMID:1082827", "title": "Co-operation between macrophages and a factor from lymphocytes in tumor lysis in vitro.", "content": "Peritoneal cells from C3H/He mice immunized against syngeneic MM46 ascites tumor cells specifically lysed these tumor cells in vitro, while spleen cells or lymph node cells from immunized hosts were completely ineffective. The effector cells differed from lymphocytes in their morphological characteristics, property of adherence, phagocytic activity, and sensitivity to anti-theta serum. The cytolytic action of the peritoneal cells was not due to cytotoxic lymphocytes, but to macrophages. These macrophages lysed tumor cells in co-operation with a factor derived from immunoglobulin-bearing lymphocytes. Neither macrophages from immunized hosts nor the factor from lymphocytes alone had a cytolytic action on the target cells. Normal macrophages showed some cytolytic activity in the presence of the factor from lymphocytes.", "contents": "Co-operation between macrophages and a factor from lymphocytes in tumor lysis in vitro. Peritoneal cells from C3H/He mice immunized against syngeneic MM46 ascites tumor cells specifically lysed these tumor cells in vitro, while spleen cells or lymph node cells from immunized hosts were completely ineffective. The effector cells differed from lymphocytes in their morphological characteristics, property of adherence, phagocytic activity, and sensitivity to anti-theta serum. The cytolytic action of the peritoneal cells was not due to cytotoxic lymphocytes, but to macrophages. These macrophages lysed tumor cells in co-operation with a factor derived from immunoglobulin-bearing lymphocytes. Neither macrophages from immunized hosts nor the factor from lymphocytes alone had a cytolytic action on the target cells. Normal macrophages showed some cytolytic activity in the presence of the factor from lymphocytes."}
{"id": "PMID:1082823", "title": "[Influence of substances that change the serotonin level of the brain on the analgesic effect of promedol in rats].", "content": "In tests with determination of pain reaction in rats registered by their squeak and arising in reponse to a single electric irritation of the tail evidence was obtained to the effect that substances both lowering the serotonin content in the brain (p-chlorphenylalanine, reserpine) and raising it (5-oxytryptophan, pargilin) cause a fall of the threshold, viz. 5-oxytryptophan and pargilin display antagonism to the analgetic effect of trimeperidine hydrochloride, whereas p-chlorphenylalanine, and inhibitor of trypophan-hydroxylase and tipindol, an antagonist of serotonin, do not exert any noticeable influence on the extent of the analgesic effect of trimeperidine hydrochloride.", "contents": "[Influence of substances that change the serotonin level of the brain on the analgesic effect of promedol in rats]. In tests with determination of pain reaction in rats registered by their squeak and arising in reponse to a single electric irritation of the tail evidence was obtained to the effect that substances both lowering the serotonin content in the brain (p-chlorphenylalanine, reserpine) and raising it (5-oxytryptophan, pargilin) cause a fall of the threshold, viz. 5-oxytryptophan and pargilin display antagonism to the analgetic effect of trimeperidine hydrochloride, whereas p-chlorphenylalanine, and inhibitor of trypophan-hydroxylase and tipindol, an antagonist of serotonin, do not exert any noticeable influence on the extent of the analgesic effect of trimeperidine hydrochloride."}
{"id": "PMID:1082828", "title": "Upper gastrointestinal endoscopy in the pediatric patient.", "content": "The value of upper gastrointestinal endoscopy in adults is well established. This study of 50 children demonstrates that upper gastrointestinal endoscopy can be performed safely and effectively in this age group without general anesthesia. Endoscopy appears more sensitive than radiology in the detection not only of superficial mucosal lesions, but of gastric and duodenal ulcers as well.", "contents": "Upper gastrointestinal endoscopy in the pediatric patient. The value of upper gastrointestinal endoscopy in adults is well established. This study of 50 children demonstrates that upper gastrointestinal endoscopy can be performed safely and effectively in this age group without general anesthesia. Endoscopy appears more sensitive than radiology in the detection not only of superficial mucosal lesions, but of gastric and duodenal ulcers as well."}
{"id": "PMID:1082830", "title": "Acute hemorrhagic incarceration of prolapsed gastric mucosa.", "content": "In some patients with hiatus hernia, vomiting may propel a plug of redundant gastric mucosa into the distal esophagus, where it becomes incarcerated and engorged. Significant bleeding may result. Reduction of the hernia controls the hemorrhage. Endoscopy after the acute episode may demonstrate a gastric mucosal lesion considered to be characteristic of the syndrome.", "contents": "Acute hemorrhagic incarceration of prolapsed gastric mucosa. In some patients with hiatus hernia, vomiting may propel a plug of redundant gastric mucosa into the distal esophagus, where it becomes incarcerated and engorged. Significant bleeding may result. Reduction of the hernia controls the hemorrhage. Endoscopy after the acute episode may demonstrate a gastric mucosal lesion considered to be characteristic of the syndrome."}
{"id": "PMID:1082832", "title": "[Anti-inflammatory, analgesic and antipyretic actions of 1-(m-chlorophenyl)-3-N, N-dimethylcarbamoyl-5-methoxypyrazole (PZ-177)].", "content": "We have reported that PZ-177 was found to have potent inhibitory activity on acute inflammatory edema. In this paper, the anti-inflammatory, analgesic and antipyretic properties of PZ-177 were assessed by a battery of standard tests. PZ-177 inhibited the increased vascular permeability induced by histamine, xylene and acetic acid. The activity was the same as the anti-edematous one and was more potent than that of mepirizole. PZ-177 did not inhibit ultraviolet erythema in guinea pigs, proliferation of granulation tissue in cotton pellet and granuloma pouch tests of rats and adjuvant arthritis in rats. Wound healing was not prolonged and the agent was weak in ulcerogenic action. PZ-177 did not affect heat denaturation of bovine serum albumin at pH 5.3, but inhibited hyperthermic hemolysis at pH 7.4 and exerted a stabilizing effect on biological membranes. This is considered to be one of the mechanisms of action. When analgesic action was tested by the writhing and Haffner's methods in mice, the compound revealed a more potent activity than did mepirizole and aminopyrine. Utilizing the Randall-Selitto's analgesic method in rats, a significant rise in pain threshold was obtained only at the inflamed foot. The antipyretic action was less than aminopyrine in febrile rabbits. From the above results, PZ-177 may be classified as a potent analgesic and anti-inflammatory agent but has no effect on proliferation of granulation tissue and chronic inflammatory disease.", "contents": "[Anti-inflammatory, analgesic and antipyretic actions of 1-(m-chlorophenyl)-3-N, N-dimethylcarbamoyl-5-methoxypyrazole (PZ-177)]. We have reported that PZ-177 was found to have potent inhibitory activity on acute inflammatory edema. In this paper, the anti-inflammatory, analgesic and antipyretic properties of PZ-177 were assessed by a battery of standard tests. PZ-177 inhibited the increased vascular permeability induced by histamine, xylene and acetic acid. The activity was the same as the anti-edematous one and was more potent than that of mepirizole. PZ-177 did not inhibit ultraviolet erythema in guinea pigs, proliferation of granulation tissue in cotton pellet and granuloma pouch tests of rats and adjuvant arthritis in rats. Wound healing was not prolonged and the agent was weak in ulcerogenic action. PZ-177 did not affect heat denaturation of bovine serum albumin at pH 5.3, but inhibited hyperthermic hemolysis at pH 7.4 and exerted a stabilizing effect on biological membranes. This is considered to be one of the mechanisms of action. When analgesic action was tested by the writhing and Haffner's methods in mice, the compound revealed a more potent activity than did mepirizole and aminopyrine. Utilizing the Randall-Selitto's analgesic method in rats, a significant rise in pain threshold was obtained only at the inflamed foot. The antipyretic action was less than aminopyrine in febrile rabbits. From the above results, PZ-177 may be classified as a potent analgesic and anti-inflammatory agent but has no effect on proliferation of granulation tissue and chronic inflammatory disease."}
{"id": "PMID:1082833", "title": "[Pharmacological studies of 2-(5H-(1)benzopyrano(2,3-b)pyridin-7-yl)propionic acid (Y-8004). (I) Anti-inflammatory, analgesic and antipyretic actions].", "content": "A newly synthesized anti-inflammatory agent, Y-8004 demonstrated a greater inhibition than did indomethacin (IM). on inflammatory response such as ultraviolet erythema in guinea pigs, carrageenin edema, evans blue and carrageenin-induced pleuritis and acetic acid-induced peritonitis in rats. On the other hand, the inhibition of Y-8004 was to the same extent as IM regarding granuloma formation around cotton pellet and the development of adjuvant arthritis in rats, however, Y-8004 was only half as effective as IM regarding exudation in granuloma pouch. In addition, the agent was effective in alleviating established adjuvant arthritis, the anti-inflammatory activity was not mediated by stimulation of the adrenals, and glucocorticoid-like activity could not be demonstrated. Furthermore, analgesic and antipyretic activities were to the degree as seen with IM, and the analgesic property of Y-8004 was considered to be peripheral as is the case with IM. The ulcerogenic activity of Y-8004 was observed to be mainly in the jejunum and ileum of the rat, but compared to IM, there were considerable differences between the ulcerogenic and the effective doses. As Y-8004 has a wider therapeutic margin than IM, this non-steroidal anti-inflammatory agent should be quite applicable for clinical purposes.", "contents": "[Pharmacological studies of 2-(5H-(1)benzopyrano(2,3-b)pyridin-7-yl)propionic acid (Y-8004). (I) Anti-inflammatory, analgesic and antipyretic actions]. A newly synthesized anti-inflammatory agent, Y-8004 demonstrated a greater inhibition than did indomethacin (IM). on inflammatory response such as ultraviolet erythema in guinea pigs, carrageenin edema, evans blue and carrageenin-induced pleuritis and acetic acid-induced peritonitis in rats. On the other hand, the inhibition of Y-8004 was to the same extent as IM regarding granuloma formation around cotton pellet and the development of adjuvant arthritis in rats, however, Y-8004 was only half as effective as IM regarding exudation in granuloma pouch. In addition, the agent was effective in alleviating established adjuvant arthritis, the anti-inflammatory activity was not mediated by stimulation of the adrenals, and glucocorticoid-like activity could not be demonstrated. Furthermore, analgesic and antipyretic activities were to the degree as seen with IM, and the analgesic property of Y-8004 was considered to be peripheral as is the case with IM. The ulcerogenic activity of Y-8004 was observed to be mainly in the jejunum and ileum of the rat, but compared to IM, there were considerable differences between the ulcerogenic and the effective doses. As Y-8004 has a wider therapeutic margin than IM, this non-steroidal anti-inflammatory agent should be quite applicable for clinical purposes."}
{"id": "PMID:1082850", "title": "[Possible role of immune-phenomena in the pathogenesis of rheumatoid arthritis (author's transl)].", "content": "The R.A. is accompanied by a variety of different immune-phenomena. These are not restricted to the well known and diagnostically exploited IgM-rheumatoid factors in serum, but involve also rheumatoid factors of the Ig-classes G and A, which, at least partially are produced by lymphoid cells, which infiltrate the synovial membranes. These cellular infiltrations consist of macrophages, B and T lymphocytes, the latter being in excess. There is now good evidence that locally deposited, complement activating immune-complexes participate in the inflammatory lesions of the R.A.-joints by inducing the release of hydrolases from phagocytic cells. This event is also mediated by lymphokines and complement factors. Is is suggested that the pathogenicity of the immunoreactions occuring within the synovial tissue and fluid is a quantitative rather than a qualitative problem. In contrast to other tissues, the anatomy of the joint may favour the local accumulation of the immune-complexes. Moreover abundant immuno-reactions may be provided by a defective T-cell mediated control. Thus there is strong indication that immune-phenomena play an important role in the pathogenesis of R.A. This does not exclude the significance of metaboltic disturbances of the connective tissues for this disease.", "contents": "[Possible role of immune-phenomena in the pathogenesis of rheumatoid arthritis (author's transl)]. The R.A. is accompanied by a variety of different immune-phenomena. These are not restricted to the well known and diagnostically exploited IgM-rheumatoid factors in serum, but involve also rheumatoid factors of the Ig-classes G and A, which, at least partially are produced by lymphoid cells, which infiltrate the synovial membranes. These cellular infiltrations consist of macrophages, B and T lymphocytes, the latter being in excess. There is now good evidence that locally deposited, complement activating immune-complexes participate in the inflammatory lesions of the R.A.-joints by inducing the release of hydrolases from phagocytic cells. This event is also mediated by lymphokines and complement factors. Is is suggested that the pathogenicity of the immunoreactions occuring within the synovial tissue and fluid is a quantitative rather than a qualitative problem. In contrast to other tissues, the anatomy of the joint may favour the local accumulation of the immune-complexes. Moreover abundant immuno-reactions may be provided by a defective T-cell mediated control. Thus there is strong indication that immune-phenomena play an important role in the pathogenesis of R.A. This does not exclude the significance of metaboltic disturbances of the connective tissues for this disease."}
{"id": "PMID:1082851", "title": "Enumeration of polyclonal mitogen-responsive cells in different lymphoid tissues of the mouse.", "content": "The relative number of cells capable of responding to Con A, PHA and LPS in the spleen, blood, lymph node and Peyer's patches of CBA mice has been quantified by means of a cytological analysis technique. No difference has been found between Con A- and PHA-responsive cells in spleen and lymph node. The lymphoid tissues of T cell-deprived mice have a reduced content of PHA responsive cells, but LPS responsiveness is within normal limits. Pretreatment of peripheral lymphocyte populations with high concentrations of anti-O antiserum and complement abolishes the response of the treated cells to PHA, but not to LPS, whereas similar treatment with a cytotoxic anti-immunoglobulin serum, which has no effect on PHA-responsive cells, only partially reduces the response to LPS. The results for mitogen responsiveness are discussed with reference to other methods of quantifying T and B cells using cell-surface markers.", "contents": "Enumeration of polyclonal mitogen-responsive cells in different lymphoid tissues of the mouse. The relative number of cells capable of responding to Con A, PHA and LPS in the spleen, blood, lymph node and Peyer's patches of CBA mice has been quantified by means of a cytological analysis technique. No difference has been found between Con A- and PHA-responsive cells in spleen and lymph node. The lymphoid tissues of T cell-deprived mice have a reduced content of PHA responsive cells, but LPS responsiveness is within normal limits. Pretreatment of peripheral lymphocyte populations with high concentrations of anti-O antiserum and complement abolishes the response of the treated cells to PHA, but not to LPS, whereas similar treatment with a cytotoxic anti-immunoglobulin serum, which has no effect on PHA-responsive cells, only partially reduces the response to LPS. The results for mitogen responsiveness are discussed with reference to other methods of quantifying T and B cells using cell-surface markers."}
{"id": "PMID:1082852", "title": "Dextran-triggered T cells heighten T- and B-cell reactions to mitogens.", "content": "Dextran administered to mice of six strains has been found to cause enhanced responses of spleen cells to the mitogens Con A and LPS. When added to cultures of spleen cells in vitro in a wide range of concentrations dextran, alone, was neither mitogenic nor toxic. The enhancement is mediated through T cells since it was absent in spleen cells from dextrantreated athymic mice. Furthermore, for the enhanced response to LPS, T cells from dextran-treated mice apparently must be in close association with B cells for longer than 2 h. The enhancement is accomplished through a humoral factor(s) produced by splenic T cells. The T cell-derived enhancing factor(s) has been shown to affect allogeneic as well as isogeneic T and B cells.", "contents": "Dextran-triggered T cells heighten T- and B-cell reactions to mitogens. Dextran administered to mice of six strains has been found to cause enhanced responses of spleen cells to the mitogens Con A and LPS. When added to cultures of spleen cells in vitro in a wide range of concentrations dextran, alone, was neither mitogenic nor toxic. The enhancement is mediated through T cells since it was absent in spleen cells from dextrantreated athymic mice. Furthermore, for the enhanced response to LPS, T cells from dextran-treated mice apparently must be in close association with B cells for longer than 2 h. The enhancement is accomplished through a humoral factor(s) produced by splenic T cells. The T cell-derived enhancing factor(s) has been shown to affect allogeneic as well as isogeneic T and B cells."}
{"id": "PMID:1082853", "title": "Characterization of dextran-activated T-cell factors.", "content": "Dextran given to mice caused splenic T cells to elaborate factors in vitro which heightened normal splenic B- and T-cell responses to the mitogens LPS and Con A, respectively. Chromatographic separation of dextran-triggered spleen cell supernatants revealed two T cell-derived enhancing factors which affect T cells (TDEF-TI and TDEF-TII) and two that alter B cells (TDEF-BI and TDEF-BII). All appear to be proteinaceous because exposure to trypsin destroyed their activities. Furthermore, their presence was found to be dependent upon protein synthesis since cycloheximide treatment of the cells inhibited synthesis whereas mitomycin C treatment did not. Based on absorption studies, receptors for TDEF-TI and TII were detected on thymic cells as well as on O-deficient bone marrow cells, whereas receptors for TDEF-BI and BII were on bone marrow cells but not thymic cells.", "contents": "Characterization of dextran-activated T-cell factors. Dextran given to mice caused splenic T cells to elaborate factors in vitro which heightened normal splenic B- and T-cell responses to the mitogens LPS and Con A, respectively. Chromatographic separation of dextran-triggered spleen cell supernatants revealed two T cell-derived enhancing factors which affect T cells (TDEF-TI and TDEF-TII) and two that alter B cells (TDEF-BI and TDEF-BII). All appear to be proteinaceous because exposure to trypsin destroyed their activities. Furthermore, their presence was found to be dependent upon protein synthesis since cycloheximide treatment of the cells inhibited synthesis whereas mitomycin C treatment did not. Based on absorption studies, receptors for TDEF-TI and TII were detected on thymic cells as well as on O-deficient bone marrow cells, whereas receptors for TDEF-BI and BII were on bone marrow cells but not thymic cells."}
{"id": "PMID:1082854", "title": "Immunochemical characterization of the anti-RNA antibodies found in scleroderma and systemic lupus erythematosus. II. Reactivity with hsa-coupled, uridine-containing, monophosphoric ribodinucleotides.", "content": "Sera from patients with scleroderma have been found to have anti-RNA antibodies which react with human serum albumin (HSA)-coupled uridine and uridine monophosphate (UMP) and are inhibited by uracil, uridine and UMP. Scleroderma sera react uniformly with 5'-polyuridylic acid (poly(U)) and fail to react with polyadenylic, polyuridylic acid poly(A) - poly(U)) which is also indicative of their uracil specificity. Anti-RNA antibodies found in systemic lupus erythematosus (SLE) are immunochemically different from those found in scleroderma in that, instead of being uniformly specific to uracil, they are markedly heterogeneous and may react with uracil, uridine and/or UMP. SLE sera frequently react with poly(A) - poly(U), indicating also their ability to recognize the double helical structure of double-stranded RNA. Thirty-seven scleroderma and thirty-four SLE sera from as many patients with either of these conditions were tested against HSA-coupled, uridine-containing monophosphoric dinucleotides in an attempt to characterize further their anti-RNA antibodies. Scleroderma sera were found to react primarily with dinucleotides in which uridine was the base proximal to the carrier protein and, except for sera that also contained antibodies to adenosine which reacted with UpA, they failed to react with dinucleotides in which uridine was in a terminal position only. Reaction with dinucleotides in which uridine was proximal to the carrier protein could be inhibited by uracil but not by the corresponding terminal base. Some lupus sera were found to react with both dinucleotides that contain the same bases in opposite sequence, e.g. ApU and UpA, while others were found to react with only one of the sequences. They were also found to react more frequently with dinucleotides in which HSA was coupled to a base other than uridine, suggesting that the reaction is primarily due to anti-DNA antibodies. Because immunization with dinucleotides coupled to protein prepared by the same method we have used, yields higher specificity to the base attached to the carrier protein, our findings suggest that, in scleroderma, a single event, akin to that of immunization with a purified antigen, gives rise to the anti-RNA antibodies, whereas in systemic lupus erythematosus there is a considerably wider immunological aberration.", "contents": "Immunochemical characterization of the anti-RNA antibodies found in scleroderma and systemic lupus erythematosus. II. Reactivity with hsa-coupled, uridine-containing, monophosphoric ribodinucleotides. Sera from patients with scleroderma have been found to have anti-RNA antibodies which react with human serum albumin (HSA)-coupled uridine and uridine monophosphate (UMP) and are inhibited by uracil, uridine and UMP. Scleroderma sera react uniformly with 5'-polyuridylic acid (poly(U)) and fail to react with polyadenylic, polyuridylic acid poly(A) - poly(U)) which is also indicative of their uracil specificity. Anti-RNA antibodies found in systemic lupus erythematosus (SLE) are immunochemically different from those found in scleroderma in that, instead of being uniformly specific to uracil, they are markedly heterogeneous and may react with uracil, uridine and/or UMP. SLE sera frequently react with poly(A) - poly(U), indicating also their ability to recognize the double helical structure of double-stranded RNA. Thirty-seven scleroderma and thirty-four SLE sera from as many patients with either of these conditions were tested against HSA-coupled, uridine-containing monophosphoric dinucleotides in an attempt to characterize further their anti-RNA antibodies. Scleroderma sera were found to react primarily with dinucleotides in which uridine was the base proximal to the carrier protein and, except for sera that also contained antibodies to adenosine which reacted with UpA, they failed to react with dinucleotides in which uridine was in a terminal position only. Reaction with dinucleotides in which uridine was proximal to the carrier protein could be inhibited by uracil but not by the corresponding terminal base. Some lupus sera were found to react with both dinucleotides that contain the same bases in opposite sequence, e.g. ApU and UpA, while others were found to react with only one of the sequences. They were also found to react more frequently with dinucleotides in which HSA was coupled to a base other than uridine, suggesting that the reaction is primarily due to anti-DNA antibodies. Because immunization with dinucleotides coupled to protein prepared by the same method we have used, yields higher specificity to the base attached to the carrier protein, our findings suggest that, in scleroderma, a single event, akin to that of immunization with a purified antigen, gives rise to the anti-RNA antibodies, whereas in systemic lupus erythematosus there is a considerably wider immunological aberration."}
{"id": "PMID:1082857", "title": "Lymphoblastic lymphoma of convoluted or acid phosphatase type-a tumor of T precursor cells.", "content": "Five lymphatic neoplasms with strong focal acid phosphatase reactivity were selected from a group of acute lymphocytic leukemias and lymphoblastic lymphomas. All five cases showed an anterior mediastinal mass and exhibited identical morphology. This type of lymphoma has been described by Lukes under the term \"malignant lymphoma of convoluted lymphocytes\". Analysis of surface membrane receptors revealed that the tumor cells lacked surface immunoglobulin and receptors for Fc-fragment, but possessed receptors for complement (C3), untreated SRBC (ES) and SRBC treated with neuraminidase (ESN). By applying a mixed rosette assay using nucleated chicken erythrocytes coated with antibodies and C3, and denucleated ESN, it was found that a considerable number of tumor cells in all five cases formed mixed rosettes, i.e. that they bore the C3 receptor characteristic of B cells and simultaneously the E receptor characteristic of T cells. Thus the tumor cells resembled immature thymocytes of 10-15 weeks' gestation, which also show focal acid phosphatase reactivity and simultaneous expression of C3 and E receptors.", "contents": "Lymphoblastic lymphoma of convoluted or acid phosphatase type-a tumor of T precursor cells. Five lymphatic neoplasms with strong focal acid phosphatase reactivity were selected from a group of acute lymphocytic leukemias and lymphoblastic lymphomas. All five cases showed an anterior mediastinal mass and exhibited identical morphology. This type of lymphoma has been described by Lukes under the term \"malignant lymphoma of convoluted lymphocytes\". Analysis of surface membrane receptors revealed that the tumor cells lacked surface immunoglobulin and receptors for Fc-fragment, but possessed receptors for complement (C3), untreated SRBC (ES) and SRBC treated with neuraminidase (ESN). By applying a mixed rosette assay using nucleated chicken erythrocytes coated with antibodies and C3, and denucleated ESN, it was found that a considerable number of tumor cells in all five cases formed mixed rosettes, i.e. that they bore the C3 receptor characteristic of B cells and simultaneously the E receptor characteristic of T cells. Thus the tumor cells resembled immature thymocytes of 10-15 weeks' gestation, which also show focal acid phosphatase reactivity and simultaneous expression of C3 and E receptors."}
{"id": "PMID:1082861", "title": "Electron transfer oxidation of DNA radicals by paranitroacetophenone.", "content": "The reaction of a typical electron-affinic sensitizer, paranitroacetophenone (PNAP) with the model compounds thymine, thymidine, thymidylic acid, deoxyribose and single and double-stranded DNA has been investigated by pulse radiolysis. Radicals formed by one-electron reduction of the bases and of DNA react rapidly and efficiently with PNAP by electron transfer. A small yield of transfer (less than 10 per cent) is also observed arising from oxidation of the radicals formed by the small proportion of OH which react at the sugar moieties in DNA. In contrast, electron transfer oxidation by PNAP of radicals formed by the addition of OH to the base moieties, e.g. thymine, is not an efficient process. Further, addition of the sensitizer to the thymine OH-adduct proceeds at a rate that is too low to measure by pulse radiolysis. We conclude that, since the major sites of OH reaction with DNA are the heterocyclic bases (greater than 80 per cent), oxidation of the resultant radicals is unlikely to be a major step in the mechanism of sensitization by this typical hypoxic-cell sensitizer.", "contents": "Electron transfer oxidation of DNA radicals by paranitroacetophenone. The reaction of a typical electron-affinic sensitizer, paranitroacetophenone (PNAP) with the model compounds thymine, thymidine, thymidylic acid, deoxyribose and single and double-stranded DNA has been investigated by pulse radiolysis. Radicals formed by one-electron reduction of the bases and of DNA react rapidly and efficiently with PNAP by electron transfer. A small yield of transfer (less than 10 per cent) is also observed arising from oxidation of the radicals formed by the small proportion of OH which react at the sugar moieties in DNA. In contrast, electron transfer oxidation by PNAP of radicals formed by the addition of OH to the base moieties, e.g. thymine, is not an efficient process. Further, addition of the sensitizer to the thymine OH-adduct proceeds at a rate that is too low to measure by pulse radiolysis. We conclude that, since the major sites of OH reaction with DNA are the heterocyclic bases (greater than 80 per cent), oxidation of the resultant radicals is unlikely to be a major step in the mechanism of sensitization by this typical hypoxic-cell sensitizer."}
{"id": "PMID:1082862", "title": "Determination of the number of photoreactivating enzyme molecules per haploid Saccharomyces cells.", "content": "Two haploid radiation-sensitive mutants of Saccharomyces were studied to investigate the formation of complex between photoreactivating-enzyme and substrate after ultra-violet irradiation. Using photo-flashes, the time necessary for maximum complex formation has been determined. Within 1 min, 70 per cent of the complexes have been formed. To determine the number of photoreactivating enzyme molecules per cell, the maximum dose decrement obtained after one photo-flash was determined and corrected for the effects of non-photoreactivable lesions. The corrected maximum dose decrement was found to be identical for both strains (8-5 erg mm-2). The number of photoreactivating-enzyme molecules involved in the photorepair of nuclear DNA damage was calculated as 272 +/- 27.", "contents": "Determination of the number of photoreactivating enzyme molecules per haploid Saccharomyces cells. Two haploid radiation-sensitive mutants of Saccharomyces were studied to investigate the formation of complex between photoreactivating-enzyme and substrate after ultra-violet irradiation. Using photo-flashes, the time necessary for maximum complex formation has been determined. Within 1 min, 70 per cent of the complexes have been formed. To determine the number of photoreactivating enzyme molecules per cell, the maximum dose decrement obtained after one photo-flash was determined and corrected for the effects of non-photoreactivable lesions. The corrected maximum dose decrement was found to be identical for both strains (8-5 erg mm-2). The number of photoreactivating-enzyme molecules involved in the photorepair of nuclear DNA damage was calculated as 272 +/- 27."}
{"id": "PMID:1082863", "title": "An elusive factor affecting mutation frequency in Tradescantia stamen hairs: its influence on r.b.e.", "content": "A comparison of data from several experiments designed to determine either relative biological effectiveness (r.b.e.) or oxygen-enhancement ratios (o.e.r.) as a function of neutron energy revealed a puzzling variability in mutational response for pink mutations in Tradescantia stamen hairs. For some reason that we cannot determine, when the exposure fixtures and accompanying experimental procedures were changed as necessitated by the two studies, the expected mutation frequencies changed for X-rays and for four neutron energies. The other four neutron energies gave the expected results. As a consequence of these changes in mutation frequencies, the magnitude of r.b.e. values changed as well as the relationship of r.b.e. to neutron energy. Consequently, when r.b.e. values were determined using a closed but aerated exposure fixture instead of exposures made in free air, the resulting r.b.e. versus neutron-energy curve more closely paralleled that found by others for the inhibiton of root growth in Vicia faba.", "contents": "An elusive factor affecting mutation frequency in Tradescantia stamen hairs: its influence on r.b.e. A comparison of data from several experiments designed to determine either relative biological effectiveness (r.b.e.) or oxygen-enhancement ratios (o.e.r.) as a function of neutron energy revealed a puzzling variability in mutational response for pink mutations in Tradescantia stamen hairs. For some reason that we cannot determine, when the exposure fixtures and accompanying experimental procedures were changed as necessitated by the two studies, the expected mutation frequencies changed for X-rays and for four neutron energies. The other four neutron energies gave the expected results. As a consequence of these changes in mutation frequencies, the magnitude of r.b.e. values changed as well as the relationship of r.b.e. to neutron energy. Consequently, when r.b.e. values were determined using a closed but aerated exposure fixture instead of exposures made in free air, the resulting r.b.e. versus neutron-energy curve more closely paralleled that found by others for the inhibiton of root growth in Vicia faba."}
{"id": "PMID:1082859", "title": "Immunologic aspects of leprosy as related to leucocytic isoantibodies and platelet aggregating factors.", "content": "The incidences of various iso- and autoantibodies in a random population of 112 unselected leprosy patients is presented. Low titers of leucocytic isoantibodies and platelet aggregating factor were detected in the sera of a variable number of such patients. The leucoisoagglutinins were found in 8% of the sera of tuberculoid as well as lepromatous leprosy patients, whereas the leucoisocytotoxins were detected in a larger percentage of the lepromatous (40%) as well as tuberculoid (28%) cases. The platelet aggregating factors (PAF) were positive in 51.2% and 45% of lepromatous and tuberculoid cases respectively. Of the 21 positive sera for PAF, the antiplatelet factor by antihuman globulin consumption test could be demonstrated only in 66.6% and 50% of lepromatous and tuberculoid sera respectively. To study the frequencies of these newly detected antibodies or antibody-like factor and to compare their occurrences with other well-documented autoantibodies present in the sera of leprosy patients: cryoglobulins, antinucleoprotein antibody and thyroglobulin autoprecipitin were also studied in the sera of the same population of leprosy patients. It has been observed that the simultaneous occurrence of all these auto- and isoantibodies in the serum of one patient is a rare phenomenon. Leucocytic and platelet counts of these patients having antibodies against leucocytes and platelets were found to be within normal limits. Accordingly, it is suggested that the low levels of antileucocyte antibody and antiplatelet factor are probably harmless to the hosts. On the other hand, it is postulated that these antibodies may act as enhancing factors by being specifically adsorbed on the lymphoid cells, thus rendering them unresponsive to mitogenic stimulus in vitro. From these studies it seems that leprosy, especially the lepromatous type, is associated with some of the serological features suggestive of an autoimmune aberration.", "contents": "Immunologic aspects of leprosy as related to leucocytic isoantibodies and platelet aggregating factors. The incidences of various iso- and autoantibodies in a random population of 112 unselected leprosy patients is presented. Low titers of leucocytic isoantibodies and platelet aggregating factor were detected in the sera of a variable number of such patients. The leucoisoagglutinins were found in 8% of the sera of tuberculoid as well as lepromatous leprosy patients, whereas the leucoisocytotoxins were detected in a larger percentage of the lepromatous (40%) as well as tuberculoid (28%) cases. The platelet aggregating factors (PAF) were positive in 51.2% and 45% of lepromatous and tuberculoid cases respectively. Of the 21 positive sera for PAF, the antiplatelet factor by antihuman globulin consumption test could be demonstrated only in 66.6% and 50% of lepromatous and tuberculoid sera respectively. To study the frequencies of these newly detected antibodies or antibody-like factor and to compare their occurrences with other well-documented autoantibodies present in the sera of leprosy patients: cryoglobulins, antinucleoprotein antibody and thyroglobulin autoprecipitin were also studied in the sera of the same population of leprosy patients. It has been observed that the simultaneous occurrence of all these auto- and isoantibodies in the serum of one patient is a rare phenomenon. Leucocytic and platelet counts of these patients having antibodies against leucocytes and platelets were found to be within normal limits. Accordingly, it is suggested that the low levels of antileucocyte antibody and antiplatelet factor are probably harmless to the hosts. On the other hand, it is postulated that these antibodies may act as enhancing factors by being specifically adsorbed on the lymphoid cells, thus rendering them unresponsive to mitogenic stimulus in vitro. From these studies it seems that leprosy, especially the lepromatous type, is associated with some of the serological features suggestive of an autoimmune aberration."}
{"id": "PMID:1082864", "title": "The repair, protection and sensitization of papain with respect to inactivation by H2O2 and OH: effects of dithiothreitol, penicillamine, cystine and penicillamine disulphide.", "content": "While dithiothreitol repairs the peroxide-produced sulphenic acid derivative of papain in a fast reaction involving only one dithiothreitol molecule, penicillamine reacts with it to form papainCys25SSPen. Disulphide is also formed in the absence of peroxide (see article) by reactions of papainCys25S- AND PenS- radicals derived from -OH reactions in penicillamine-papain mixtures. A similar formation of papainCys25SSCys occurs in mixtures of cysteine and papain. However, unlike papainCys25SSCys, papainCys25SSPen cannot easily be restored to the active form of papain by the exchange reaction with CysSH, and this may have significance for an understanding of the sensitizing action of penicillamine observed in some in vivo systems. Under the action of OH radicals dithiothreitol has less of a tendency to form mixed disulphides and is more effective in repairing papain-OH intermediates than either cysteine or penicillamine. Due to secondary reactions of RSOH and other oxidized species the disulphides of cysteine and penicillamine are less effective than the sulphydryls in protecting papain against inactivation by -OH.", "contents": "The repair, protection and sensitization of papain with respect to inactivation by H2O2 and OH: effects of dithiothreitol, penicillamine, cystine and penicillamine disulphide. While dithiothreitol repairs the peroxide-produced sulphenic acid derivative of papain in a fast reaction involving only one dithiothreitol molecule, penicillamine reacts with it to form papainCys25SSPen. Disulphide is also formed in the absence of peroxide (see article) by reactions of papainCys25S- AND PenS- radicals derived from -OH reactions in penicillamine-papain mixtures. A similar formation of papainCys25SSCys occurs in mixtures of cysteine and papain. However, unlike papainCys25SSCys, papainCys25SSPen cannot easily be restored to the active form of papain by the exchange reaction with CysSH, and this may have significance for an understanding of the sensitizing action of penicillamine observed in some in vivo systems. Under the action of OH radicals dithiothreitol has less of a tendency to form mixed disulphides and is more effective in repairing papain-OH intermediates than either cysteine or penicillamine. Due to secondary reactions of RSOH and other oxidized species the disulphides of cysteine and penicillamine are less effective than the sulphydryls in protecting papain against inactivation by -OH."}
{"id": "PMID:1082865", "title": "Structural damage of gamma-irradiated ribonuclease revealed by thin-layer isoelectric focusing.", "content": "Ribonuclease, irradiated with 60Co gamma-rays in dilute aqueous solution or in the dry state, has been investigated with respect to its charge and size properties. Thin-layer isoelectric focusing revealed extensive change in irradiated RNase; new enzymatically-active components, mainly with isoelectric points lower than in unirradiated RNase were observed. Thin-layer gel chromatography indicated the formation of aggregates which are partially active enzymatically. Aggreation depended on enzyme concentration and was less in more dilute solutions-- at equal degrees of inactivation. Structural damage in the so-called 'native' monomers was revealed by thin-layer isoelectric focusing, their charge properties being distinctly modified.", "contents": "Structural damage of gamma-irradiated ribonuclease revealed by thin-layer isoelectric focusing. Ribonuclease, irradiated with 60Co gamma-rays in dilute aqueous solution or in the dry state, has been investigated with respect to its charge and size properties. Thin-layer isoelectric focusing revealed extensive change in irradiated RNase; new enzymatically-active components, mainly with isoelectric points lower than in unirradiated RNase were observed. Thin-layer gel chromatography indicated the formation of aggregates which are partially active enzymatically. Aggreation depended on enzyme concentration and was less in more dilute solutions-- at equal degrees of inactivation. Structural damage in the so-called 'native' monomers was revealed by thin-layer isoelectric focusing, their charge properties being distinctly modified."}
{"id": "PMID:1082886", "title": "Extrachromosomal deoxyribonucleic acid in wild-type and photosynthetically incompetent strains of Rhodopseudomonas spheroides.", "content": "Three covalently closed circular species of extrachromosomal deoxyribonucleic acid have been identified by electron microscopic analysis in strains of Rhodopseudomonas spheroides. The weights of these plasmids, as determined from contour length, are about 75 X 10(6), 66 X 10(6), and 28 X 10(6) daltons for both aerobically grown and photosynthetically grown R. spheroides strain 2.4.1 (NRS) and for the photosynthetically incompetent strain V-2 (obtained by N-methyl-N-nitro-N'nitrosoguanidine mutagenesis) and 74 X 10(6), 66 X 10(6) and 34 X 10(6) daltons for a second photosynthetically incompetent strain, SLS I (obtained by incubating strain 2.4.1 [NRS] in medium containing sodium lauryl sulfate). Buoyant densities uere found to be 1.717 g/cm3 (58% guanine plus cytosine) for the plasmids of 66 X 10(6), 28 X 10(6), and 34 X 10(6) daltons in weight and 1.724 g/cm3 (65% guanine plus cytosine) for those weighing about 75 X 10(6) daltons. Possible functions of these plasmids are discussed.", "contents": "Extrachromosomal deoxyribonucleic acid in wild-type and photosynthetically incompetent strains of Rhodopseudomonas spheroides. Three covalently closed circular species of extrachromosomal deoxyribonucleic acid have been identified by electron microscopic analysis in strains of Rhodopseudomonas spheroides. The weights of these plasmids, as determined from contour length, are about 75 X 10(6), 66 X 10(6), and 28 X 10(6) daltons for both aerobically grown and photosynthetically grown R. spheroides strain 2.4.1 (NRS) and for the photosynthetically incompetent strain V-2 (obtained by N-methyl-N-nitro-N'nitrosoguanidine mutagenesis) and 74 X 10(6), 66 X 10(6) and 34 X 10(6) daltons for a second photosynthetically incompetent strain, SLS I (obtained by incubating strain 2.4.1 [NRS] in medium containing sodium lauryl sulfate). Buoyant densities uere found to be 1.717 g/cm3 (58% guanine plus cytosine) for the plasmids of 66 X 10(6), 28 X 10(6), and 34 X 10(6) daltons in weight and 1.724 g/cm3 (65% guanine plus cytosine) for those weighing about 75 X 10(6) daltons. Possible functions of these plasmids are discussed."}
{"id": "PMID:1082887", "title": "Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin.", "content": "We have found that PHA produces an alteration in the lymphocyte membrane which allows 86Rb+ or 42K+ in prelabeled lymphocytes to exchange for cations present in washing solutions. These observations suggested that PHA might induce an increase in the exodus of intracellular potassium during incubation in physiologic media. We, therefore, examined 86Rb+ and 42K+ efflux from rat and human lymphocytes during incubation in tissue culture medium. The rate constant for efflux, Ke, was significantly increased by PHA. 86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment.", "contents": "Exodus of 42K+ and 86Rb+ from rat thymic and human blood lymphocytes exposed to phytohemagglutinin. We have found that PHA produces an alteration in the lymphocyte membrane which allows 86Rb+ or 42K+ in prelabeled lymphocytes to exchange for cations present in washing solutions. These observations suggested that PHA might induce an increase in the exodus of intracellular potassium during incubation in physiologic media. We, therefore, examined 86Rb+ and 42K+ efflux from rat and human lymphocytes during incubation in tissue culture medium. The rate constant for efflux, Ke, was significantly increased by PHA. 86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment."}
{"id": "PMID:1082888", "title": "Suppressor thymus-derived lymphocytes in fungal infection.", "content": "Thymus-derived lymphocyte (T-cell) function, as determined in vivo by cutaneous reactivity to several antigens and in vitro by responsiveness to mitogens and antigens, was assessed in 14 patients infected with a variety of fungal organisms. While all patients manifested a normal frequency of peripheral blood T cells, only seven patients reacted to at least one of the antigens used for cutaneous testing and demonstrated normal in vitro T proliferative responses. Three patients exhibited cutaneous anergy but normal in vitro T-cell reactivity while four patients demonstrated persistent anergy and marked in vitro T-cell hyporeactivity which was independent of activity of infection, concurrent medication, or any associated disorders. The marked diminution of in vitro T-cell reactivity noted for these later four patients was not due to a deletion of antigen- or mitogen-reactive cells. Thus, patients' cells which had been initially cultured for 7 days without any mitogenic or antigenic stimulus and which were subsequently washed and recultured with phytohemagglutinin, concanavalin A, or histoplasmin demonstrated a marked increase in their responsiveness. Moreover, this reactivity noted for recultured cells could be suppressed by a nonphagocytic, nonadherent, nonimmunoglobulin-bearing, sheep red blood cell rosette-forming population of cells isolated from the fresh peripheral blood mononuclear cells of the same patient. While these \"regulator\" T cells were capable of suppressing T-proliferative responses to antigens and mitogens, they did not diminish pokeweed mitogen-induced immunoglobulin synthesis by normal bone marrow-derived lymphocytes. Patients in whom suppressor \"T\" cells were found were at risk for relapsing, disseminated fungal infection.", "contents": "Suppressor thymus-derived lymphocytes in fungal infection. Thymus-derived lymphocyte (T-cell) function, as determined in vivo by cutaneous reactivity to several antigens and in vitro by responsiveness to mitogens and antigens, was assessed in 14 patients infected with a variety of fungal organisms. While all patients manifested a normal frequency of peripheral blood T cells, only seven patients reacted to at least one of the antigens used for cutaneous testing and demonstrated normal in vitro T proliferative responses. Three patients exhibited cutaneous anergy but normal in vitro T-cell reactivity while four patients demonstrated persistent anergy and marked in vitro T-cell hyporeactivity which was independent of activity of infection, concurrent medication, or any associated disorders. The marked diminution of in vitro T-cell reactivity noted for these later four patients was not due to a deletion of antigen- or mitogen-reactive cells. Thus, patients' cells which had been initially cultured for 7 days without any mitogenic or antigenic stimulus and which were subsequently washed and recultured with phytohemagglutinin, concanavalin A, or histoplasmin demonstrated a marked increase in their responsiveness. Moreover, this reactivity noted for recultured cells could be suppressed by a nonphagocytic, nonadherent, nonimmunoglobulin-bearing, sheep red blood cell rosette-forming population of cells isolated from the fresh peripheral blood mononuclear cells of the same patient. While these \"regulator\" T cells were capable of suppressing T-proliferative responses to antigens and mitogens, they did not diminish pokeweed mitogen-induced immunoglobulin synthesis by normal bone marrow-derived lymphocytes. Patients in whom suppressor \"T\" cells were found were at risk for relapsing, disseminated fungal infection."}
{"id": "PMID:1082889", "title": "Role of mercaptoethanol and endotoxin in stimulating B lymphocyte colony formation in vitro.", "content": "Mercaptoethanol is necessary to permit B lymphocyte colony formation in semi-solid agar cultures of cells from normal mouse lymphoid organs. Transfer studies on developing colonies showed that, in part, this was a direct action on B lymphocyte colony cells but evidence was produced that in the presence of mercaptoethanol lymphoid organ cells release a factor promoting colony growth. Endotoxin strongly potentiated B lymphocyte colony formation in vitro by a direct action on colony cells but in the absence of mercaptoethanol did not allow cell survival or proliferation.", "contents": "Role of mercaptoethanol and endotoxin in stimulating B lymphocyte colony formation in vitro. Mercaptoethanol is necessary to permit B lymphocyte colony formation in semi-solid agar cultures of cells from normal mouse lymphoid organs. Transfer studies on developing colonies showed that, in part, this was a direct action on B lymphocyte colony cells but evidence was produced that in the presence of mercaptoethanol lymphoid organ cells release a factor promoting colony growth. Endotoxin strongly potentiated B lymphocyte colony formation in vitro by a direct action on colony cells but in the absence of mercaptoethanol did not allow cell survival or proliferation."}
{"id": "PMID:1082890", "title": "Separation of lymphocyte mitogen from lymphotoxin and experiments on the production of lymphotoxin by lymphoid cells stimulated with the partially purified mitogen: a possible amplification mechanism of cellular immunity and allergy.", "content": "A mitogenic factor (MF) from guinea pig lymph node cells (LNC), which was produced by stimulating immune LNC with the specific antigen, ovalbumin, was partially purified by the sequential use of Sephadex G-200 gel filtration. CM-cellulose ion exchange chromatography, DEAE-cellulose ion exchange chromatography, and polyacrylamide disc gel electrophoresis. The product was purified at least 100-fold with regard to protein content. In addition, the purified MF was functionally pure with respect to the absence fo lymphotoxin (LT), another guinea pig lymphokine.", "contents": "Separation of lymphocyte mitogen from lymphotoxin and experiments on the production of lymphotoxin by lymphoid cells stimulated with the partially purified mitogen: a possible amplification mechanism of cellular immunity and allergy. A mitogenic factor (MF) from guinea pig lymph node cells (LNC), which was produced by stimulating immune LNC with the specific antigen, ovalbumin, was partially purified by the sequential use of Sephadex G-200 gel filtration. CM-cellulose ion exchange chromatography, DEAE-cellulose ion exchange chromatography, and polyacrylamide disc gel electrophoresis. The product was purified at least 100-fold with regard to protein content. In addition, the purified MF was functionally pure with respect to the absence fo lymphotoxin (LT), another guinea pig lymphokine."}
{"id": "PMID:1082891", "title": "Thymus-derived peripheral lymphocytes from human newborns inhibit division of their mothers' lymphocytes.", "content": "Lymphocytes from human newborns inhibit division of their mothers' lymphocytes. Three days after we cultured equal numbers of cells from a mother and her baby in the presence of PHA, mitosis of the mother's lymphocytes was suppressed 13-fold compared to that of the baby's lymphocytes. At the end of 3 days the number of baby's lymphocytes were doubled those of the mother's. The survival rates and mean mitotic indexes of both pairs of cell were roughly equivalent (mean +/- S.E: baby 2.4 +/- 0.8; mother 2.6 +/- 0.7), indicating that the lack of dividing lymphocytes from the mother was caused by inhibited division of the mother's lymphocytes, no enhanced growth of the newborn's cells. The cell population in newborns that is responsible for the inhibition effect resides in the T cell-enriched population. Lymphocytes from one newborn were not able to inhibit division of lymphocytes from another newborn, suggesting that lymphocytes from newborns could continue to divide despite their inhibitory effect. Other experiments showed that actively dividing fetal fibroblasts, amnion cells from the newborn, and continuous T lymphoblastoid cell lines were unable to inhibit mitosis of lymphocytes of the mother.", "contents": "Thymus-derived peripheral lymphocytes from human newborns inhibit division of their mothers' lymphocytes. Lymphocytes from human newborns inhibit division of their mothers' lymphocytes. Three days after we cultured equal numbers of cells from a mother and her baby in the presence of PHA, mitosis of the mother's lymphocytes was suppressed 13-fold compared to that of the baby's lymphocytes. At the end of 3 days the number of baby's lymphocytes were doubled those of the mother's. The survival rates and mean mitotic indexes of both pairs of cell were roughly equivalent (mean +/- S.E: baby 2.4 +/- 0.8; mother 2.6 +/- 0.7), indicating that the lack of dividing lymphocytes from the mother was caused by inhibited division of the mother's lymphocytes, no enhanced growth of the newborn's cells. The cell population in newborns that is responsible for the inhibition effect resides in the T cell-enriched population. Lymphocytes from one newborn were not able to inhibit division of lymphocytes from another newborn, suggesting that lymphocytes from newborns could continue to divide despite their inhibitory effect. Other experiments showed that actively dividing fetal fibroblasts, amnion cells from the newborn, and continuous T lymphoblastoid cell lines were unable to inhibit mitosis of lymphocytes of the mother."}
{"id": "PMID:1082892", "title": "Adjuvant regulation of T cell function.", "content": "The effect of complete Freund's adjuvant (CFA) on distinct T cell functions was investigated. Adjuvant was found to suppress the generation of cytolytic T cells in vivo when mixed with allogeneic P815 cells before immunization of C57BL/6 mice. Inoculation of the mice with either adjuvant or adjuvant emulsified with allogeneic cells resulted in whole splenic populations or immunoabsorbent-purified T cells that did not generate cytolytic activity in vitro against allogeneic cells. Mixing T cells from normal and adjuvant-treated mice before in vitro sensitization resulted in suppression of lytic activity. However, memory T cells were not subject to the same suppressive regulation as were precytotoxic T cells since adjuvant had no effect on subsequent boosting of memory.", "contents": "Adjuvant regulation of T cell function. The effect of complete Freund's adjuvant (CFA) on distinct T cell functions was investigated. Adjuvant was found to suppress the generation of cytolytic T cells in vivo when mixed with allogeneic P815 cells before immunization of C57BL/6 mice. Inoculation of the mice with either adjuvant or adjuvant emulsified with allogeneic cells resulted in whole splenic populations or immunoabsorbent-purified T cells that did not generate cytolytic activity in vitro against allogeneic cells. Mixing T cells from normal and adjuvant-treated mice before in vitro sensitization resulted in suppression of lytic activity. However, memory T cells were not subject to the same suppressive regulation as were precytotoxic T cells since adjuvant had no effect on subsequent boosting of memory."}
{"id": "PMID:1082893", "title": "Cellular immunity in pyelonephritis: identification of suppressor cell activity of spleen cells in response to concanavalin A and inhibition of lymphocyte-mediated L cell cytotoxicity.", "content": "A population of fast sedimenting spleen cells was identified as the cause of impaired in vitro response of spleen cells to concanavalin A (Con A) in acute pyelonephritis in rats. These fast-sedimenting cells responded to Con A by suppressing the DNA synthetic response of normal spleen cells to Con A. In addition, spleen cells from acute pyelonephritis rats were significantly less able to mediate in vitro cell destruction in xenogeneic aggressor lymphocyte: target L cell mixtures. On the basis of these findings, the hypothesis is proposed that a suppressor cell can protect the pyelonephritic kidney against immunologically mediated tissue damage.", "contents": "Cellular immunity in pyelonephritis: identification of suppressor cell activity of spleen cells in response to concanavalin A and inhibition of lymphocyte-mediated L cell cytotoxicity. A population of fast sedimenting spleen cells was identified as the cause of impaired in vitro response of spleen cells to concanavalin A (Con A) in acute pyelonephritis in rats. These fast-sedimenting cells responded to Con A by suppressing the DNA synthetic response of normal spleen cells to Con A. In addition, spleen cells from acute pyelonephritis rats were significantly less able to mediate in vitro cell destruction in xenogeneic aggressor lymphocyte: target L cell mixtures. On the basis of these findings, the hypothesis is proposed that a suppressor cell can protect the pyelonephritic kidney against immunologically mediated tissue damage."}
{"id": "PMID:1082894", "title": "Regualtion of the immune response to tumor antigens. I. Immunosuppressor cells in tumor-bearing hosts.", "content": "A/Jax mice were rendered immune to the syngeneic and transplantable methylcholanthrene-induced Sarcoma 1509a by the surgical removal of the tumor 7 days after implantation; subsequent injection i.v. transfer of 10(7) to 10(8) washed thymus or spleen cells of tumor-bearing animals (TBA) to immune animals significantly inhibited the rejection of the tumor; this suppressive effect was entirely abolished by the treatment of these lymphocytes with anti-theta serum or anti-thymocyte serum (ATS) and complement before adoptive transfer. On the other hand, an equal number of thymus or spleen cells of normal animals or of animals bearing an unrelated tumor had no suppressive effect. Treatment of normal syngeneic animals with ATS after tumor cell inoculation or splenectomy of TBA resulted in the suppression of the tumor growth. The serum of TBA had no effect on tumor growth in immune syngeneic mice. Together these results suggest that TBA possess immunosuppressor T cells regulating negatively their immune response to the tumor.", "contents": "Regualtion of the immune response to tumor antigens. I. Immunosuppressor cells in tumor-bearing hosts. A/Jax mice were rendered immune to the syngeneic and transplantable methylcholanthrene-induced Sarcoma 1509a by the surgical removal of the tumor 7 days after implantation; subsequent injection i.v. transfer of 10(7) to 10(8) washed thymus or spleen cells of tumor-bearing animals (TBA) to immune animals significantly inhibited the rejection of the tumor; this suppressive effect was entirely abolished by the treatment of these lymphocytes with anti-theta serum or anti-thymocyte serum (ATS) and complement before adoptive transfer. On the other hand, an equal number of thymus or spleen cells of normal animals or of animals bearing an unrelated tumor had no suppressive effect. Treatment of normal syngeneic animals with ATS after tumor cell inoculation or splenectomy of TBA resulted in the suppression of the tumor growth. The serum of TBA had no effect on tumor growth in immune syngeneic mice. Together these results suggest that TBA possess immunosuppressor T cells regulating negatively their immune response to the tumor."}
{"id": "PMID:1082895", "title": "Cytotoxic activity of lymphocytes. VI. Heterogeneity of cytotoxins in supernatants of mitogen-activated lymphocytes.", "content": "Two different lymphotoxins synthesized by human blood lymphocytes stimulated with phytohemagglutin (PHA) are described. The two toxins are called alpha-LT and beta-LT relative to their elution order on gel chromatography. Their m.w. are 75,000 and 45,000 daltons, respectively. Both toxins appear as early as 7 hr after the addition of PHA, with the amount of beta-toxin exceeding that of alpha-LT initially. Both toxins are differentiated from a third toxin (adherent cell toxin, ACT) made by plastic-adherent cells without requiring mitogen-stimulation. Depletion of macrophages or B cells does not affect the synthesis of either lymphotoxin. Monospecific antisera were elicited to alpha-LT. Antisera elicited to beta-LT also neutralized alpha-LT but to a significantly lesser degree. Alpha-LT appears to be the lymphotoxin described by most other workers. Beta-LT is unstable at 37 degrees C which may explain why the low m.w. lymphotoxin has not been described previously.", "contents": "Cytotoxic activity of lymphocytes. VI. Heterogeneity of cytotoxins in supernatants of mitogen-activated lymphocytes. Two different lymphotoxins synthesized by human blood lymphocytes stimulated with phytohemagglutin (PHA) are described. The two toxins are called alpha-LT and beta-LT relative to their elution order on gel chromatography. Their m.w. are 75,000 and 45,000 daltons, respectively. Both toxins appear as early as 7 hr after the addition of PHA, with the amount of beta-toxin exceeding that of alpha-LT initially. Both toxins are differentiated from a third toxin (adherent cell toxin, ACT) made by plastic-adherent cells without requiring mitogen-stimulation. Depletion of macrophages or B cells does not affect the synthesis of either lymphotoxin. Monospecific antisera were elicited to alpha-LT. Antisera elicited to beta-LT also neutralized alpha-LT but to a significantly lesser degree. Alpha-LT appears to be the lymphotoxin described by most other workers. Beta-LT is unstable at 37 degrees C which may explain why the low m.w. lymphotoxin has not been described previously."}
{"id": "PMID:1082896", "title": "Factors influencing the isolation of membrane immunoglobulins from T and B lymphocytes. 1. Detergent effects and iodination conditions.", "content": "The optimal conditions for the extraction and isolation of immunoglobulins from the cell membrane (M-Ig) of T and B lymphocytes radioiodinated with lactoperoxidase were studied. A combination of 10M-urea, 1.5 M acetic acid and the non-ionic detergents Triton X-100 or Nonidet P-40 (NP-40) was found to overcome the tendency for membrane proteins to precipitate after extraction in urea-acetic acid alone. However, detection of T cell Ig was more dependent on detergent concentration than B cell M-Ig. Less T cell Ig was detected when the detergent concentration was increased from 0.1% to 1.0%, whereas the amount of B cell M-Ig was not affected by higher detergent concentrations.", "contents": "Factors influencing the isolation of membrane immunoglobulins from T and B lymphocytes. 1. Detergent effects and iodination conditions. The optimal conditions for the extraction and isolation of immunoglobulins from the cell membrane (M-Ig) of T and B lymphocytes radioiodinated with lactoperoxidase were studied. A combination of 10M-urea, 1.5 M acetic acid and the non-ionic detergents Triton X-100 or Nonidet P-40 (NP-40) was found to overcome the tendency for membrane proteins to precipitate after extraction in urea-acetic acid alone. However, detection of T cell Ig was more dependent on detergent concentration than B cell M-Ig. Less T cell Ig was detected when the detergent concentration was increased from 0.1% to 1.0%, whereas the amount of B cell M-Ig was not affected by higher detergent concentrations."}
{"id": "PMID:1082897", "title": "Phytohemagglutinin-induced proliferation of guinea pig thymus-derived lymphocytes. I. Accessory cell dependence.", "content": "The accessory cell requirement for mitogen-induced T lymphocyte proliferation has been investigated by using a population of guinea pig lymph node lymphocytes enriched in T cells and markedly depleted of macrophages and B lymphocytes. We have found that effective phytohemagglutinin-induced proliferation of T cells is dependent on the participation of accessory cells. Augmentation of PHA responsiveness was noted when cultural conditions were manipulated to increase cell density, suggesting that physical proximity between T cell and accessory cell is required for efficient triggering. Both syngeneic and allogeneic macrophages, as well as syngeneic fibroblasts, serve as accessory cells in this response whereas polymorphonuclear leukocytes or thymocytes do not. Thus, although PHA-induced T lymphocyte proliferation requires accessory cells, the specificity of these cells is strikingly less stringent than for antigen-mediated triggering of immune guinea pig T cells, a response which is dependent upon participation of syngeneic macrophages.", "contents": "Phytohemagglutinin-induced proliferation of guinea pig thymus-derived lymphocytes. I. Accessory cell dependence. The accessory cell requirement for mitogen-induced T lymphocyte proliferation has been investigated by using a population of guinea pig lymph node lymphocytes enriched in T cells and markedly depleted of macrophages and B lymphocytes. We have found that effective phytohemagglutinin-induced proliferation of T cells is dependent on the participation of accessory cells. Augmentation of PHA responsiveness was noted when cultural conditions were manipulated to increase cell density, suggesting that physical proximity between T cell and accessory cell is required for efficient triggering. Both syngeneic and allogeneic macrophages, as well as syngeneic fibroblasts, serve as accessory cells in this response whereas polymorphonuclear leukocytes or thymocytes do not. Thus, although PHA-induced T lymphocyte proliferation requires accessory cells, the specificity of these cells is strikingly less stringent than for antigen-mediated triggering of immune guinea pig T cells, a response which is dependent upon participation of syngeneic macrophages."}
{"id": "PMID:1082898", "title": "Phytohemagglutinin-induced proliferation of guinea pig thymus-derived lymphocytes. II. Accessory cell function.", "content": "Accessory cell participation in PHA-induced thymus-derived lymphocyte DNA synthesis encompasses two distinct functions. The first consists of maintenance of the functional integrity of resting lymphocytes, and the second involves the direct induction and/or support of T cell proliferation in response to this mitogen. Whereas the reducing agent 2-mercaptoethanol can support an Mphi-depleted population of resting lymphocytes so that the latent biologic activity is maintained, it is not itself sufficient to allow the induction of lymphocyte proliferation in response to PHA. This latter function requires intact accessory cells.", "contents": "Phytohemagglutinin-induced proliferation of guinea pig thymus-derived lymphocytes. II. Accessory cell function. Accessory cell participation in PHA-induced thymus-derived lymphocyte DNA synthesis encompasses two distinct functions. The first consists of maintenance of the functional integrity of resting lymphocytes, and the second involves the direct induction and/or support of T cell proliferation in response to this mitogen. Whereas the reducing agent 2-mercaptoethanol can support an Mphi-depleted population of resting lymphocytes so that the latent biologic activity is maintained, it is not itself sufficient to allow the induction of lymphocyte proliferation in response to PHA. This latter function requires intact accessory cells."}
{"id": "PMID:1082900", "title": "Characterization of a subpopulation of human T lymphocytes reactive with an heteroantiserum to human brain.", "content": "A rabbit antiserum to human fetal brain reacted after suitable absorptions with a subpopulation of human normal T cells. The distribution of reactive T cells varied according to the organ tested: 23% of peripheral blood lymphocytes, 5% of tonsil lymphocytes, and less than 1% of thymocytes were positive. Reactive cells did not transform after phytohemagglutinin or pokeweed stimulation but were at least weakly stimulated by allogeneic cells. T-derived neoplastic cells from one case of T acute lymphoblastic leukemia, two patients with Sezary syndrome, and from three out of five cases of T chronic lymphocytic leukemias (CLL) yielded negative results. In contrast, all the leukemic cells from two other patients with T-derived CLL were positive suggesting a proliferation of homogeneous cells arising from only a subpopulation of T lymphocytes.", "contents": "Characterization of a subpopulation of human T lymphocytes reactive with an heteroantiserum to human brain. A rabbit antiserum to human fetal brain reacted after suitable absorptions with a subpopulation of human normal T cells. The distribution of reactive T cells varied according to the organ tested: 23% of peripheral blood lymphocytes, 5% of tonsil lymphocytes, and less than 1% of thymocytes were positive. Reactive cells did not transform after phytohemagglutinin or pokeweed stimulation but were at least weakly stimulated by allogeneic cells. T-derived neoplastic cells from one case of T acute lymphoblastic leukemia, two patients with Sezary syndrome, and from three out of five cases of T chronic lymphocytic leukemias (CLL) yielded negative results. In contrast, all the leukemic cells from two other patients with T-derived CLL were positive suggesting a proliferation of homogeneous cells arising from only a subpopulation of T lymphocytes."}
{"id": "PMID:1082901", "title": "Specific antigen-binding and antibody-secreting lymphocytes associated with the erythrocyte autoantibody responses of NZB and genetically unrelated mice.", "content": "The receptor characteristics as well as incidence of antigen-binding lymphocytes (ABL) or B and T cell classes with membrane receptors specific for the exposed (X) and cryptic (HB) murine erythrocyte autoantigens were examined in NZB and nine control strains of mice. Whereas only NZB and NZB hybrid mice synthesize anti-X autoantibody pathogenetically implicated in the genetically determined autoimmune hemolytic anemia, the NZB as well as control strains synthesize the ubiquitous anti-HB anti-erythrocyte autoantibody. By utilizing immunocytoadherence assays, maximum numbers of specific ABL of both B and T lymphocyte classes were optimally demonstrated at erythrocyte:lymphocyte ratios of 20:1 and after lymphocyte fixation at 56 degrees C for 20 min. Surface membrane receptor specificity was established by inhibition with semi-purified soluble X or HB autoantigen. Inhibition of immunocyto-adherence with class specific antisera to mouse immuno-globulins demonstrated that the receptors on both B and T cells were of IgM class. Specific receptors regenerated in vitro after trypsinization which excluded the role of cytophilic antibody in the immunocytoadherence reactions. B lymphocyte ABL reactive with the X autoantigen were demonstrable in NZB, NZB hybrid, and control mice. Only in NZB and NZB hybrid mice, strains that uniformly synthesize anti-X autoantibody, were X ABL of T lymphocyte class demonstrated. The presence and incidence of T lymphocyte X ABL is compatible with the expression of a single dominant gene carried by the NAB strain. The incidence of B lymphocyte X ABL increased with age, suggesting proliferation of this cell population. HB ABL of both B and T lymphocyte classes were observed in all strains, concordant with the ubiquitous presence of humoral anti-HB autoantibodies. Differentiation of precursor B cells are evaluated by PFC assay of cells secreting specific autoantibodies. Anti-X PFC were observed only in NZB and NZB hybrid mice; and the observed frequency suggested that less than 3.5% of the specific ABL were differentiated for the secretion of anti-X autoantibody. Anti-HB PFC were observed in all strains and represented as high as 11.8% of specific ABL. Genetic determination of the anti-X anti-erythrocyte autoantibody response does not prescribe the presence of precursors of the antibody-forming cell, but rather appears to influence regulation of the differentiation of these cells. These data suggest that circumvention of immunologic tolerance to this specific erythrocyte autoantigen may occur at the level of the T lymphocyte; or alternatively, that T lymphocytes as well as B lymphocytes, are induced to proliferate and differentiate in the NZB strain.", "contents": "Specific antigen-binding and antibody-secreting lymphocytes associated with the erythrocyte autoantibody responses of NZB and genetically unrelated mice. The receptor characteristics as well as incidence of antigen-binding lymphocytes (ABL) or B and T cell classes with membrane receptors specific for the exposed (X) and cryptic (HB) murine erythrocyte autoantigens were examined in NZB and nine control strains of mice. Whereas only NZB and NZB hybrid mice synthesize anti-X autoantibody pathogenetically implicated in the genetically determined autoimmune hemolytic anemia, the NZB as well as control strains synthesize the ubiquitous anti-HB anti-erythrocyte autoantibody. By utilizing immunocytoadherence assays, maximum numbers of specific ABL of both B and T lymphocyte classes were optimally demonstrated at erythrocyte:lymphocyte ratios of 20:1 and after lymphocyte fixation at 56 degrees C for 20 min. Surface membrane receptor specificity was established by inhibition with semi-purified soluble X or HB autoantigen. Inhibition of immunocyto-adherence with class specific antisera to mouse immuno-globulins demonstrated that the receptors on both B and T cells were of IgM class. Specific receptors regenerated in vitro after trypsinization which excluded the role of cytophilic antibody in the immunocytoadherence reactions. B lymphocyte ABL reactive with the X autoantigen were demonstrable in NZB, NZB hybrid, and control mice. Only in NZB and NZB hybrid mice, strains that uniformly synthesize anti-X autoantibody, were X ABL of T lymphocyte class demonstrated. The presence and incidence of T lymphocyte X ABL is compatible with the expression of a single dominant gene carried by the NAB strain. The incidence of B lymphocyte X ABL increased with age, suggesting proliferation of this cell population. HB ABL of both B and T lymphocyte classes were observed in all strains, concordant with the ubiquitous presence of humoral anti-HB autoantibodies. Differentiation of precursor B cells are evaluated by PFC assay of cells secreting specific autoantibodies. Anti-X PFC were observed only in NZB and NZB hybrid mice; and the observed frequency suggested that less than 3.5% of the specific ABL were differentiated for the secretion of anti-X autoantibody. Anti-HB PFC were observed in all strains and represented as high as 11.8% of specific ABL. Genetic determination of the anti-X anti-erythrocyte autoantibody response does not prescribe the presence of precursors of the antibody-forming cell, but rather appears to influence regulation of the differentiation of these cells. These data suggest that circumvention of immunologic tolerance to this specific erythrocyte autoantigen may occur at the level of the T lymphocyte; or alternatively, that T lymphocytes as well as B lymphocytes, are induced to proliferate and differentiate in the NZB strain."}
{"id": "PMID:1082902", "title": "Sensitization of T lymphocytes in vitro by syngeneic macrophages fed with tumor antigens.", "content": "We investigated the interaction between T lymphocytes and macrophages in the vitro sensitization of lymphocytes against tumor cells. Spleen cells were sensitized in vitro by syngeneic peritoneal macrophages that had been fed with cell-free antigen preparation of syngeneic tumor cells. The sensitized T lymphocytes acquired specific cytotoxic cells. The sensitized T lymphocytes acquired specific cytotoxic activity in vitro and the capacity to inhingeneic fibroblasts, or the antigen preparation by itself were not able to sensitize the lymphocytes against the tumor.", "contents": "Sensitization of T lymphocytes in vitro by syngeneic macrophages fed with tumor antigens. We investigated the interaction between T lymphocytes and macrophages in the vitro sensitization of lymphocytes against tumor cells. Spleen cells were sensitized in vitro by syngeneic peritoneal macrophages that had been fed with cell-free antigen preparation of syngeneic tumor cells. The sensitized T lymphocytes acquired specific cytotoxic cells. The sensitized T lymphocytes acquired specific cytotoxic activity in vitro and the capacity to inhingeneic fibroblasts, or the antigen preparation by itself were not able to sensitize the lymphocytes against the tumor."}
{"id": "PMID:1082903", "title": "Hereditary C2 deficiency: diagnosis and HLA gene complex associations.", "content": "Nine families with genetically controlled C2 deficiency have been described where the propositii and family members are heterozygous C2 deficient. The diagnosis of hereditary C2 heterozygous deficiency was suspected on the basis of analysis for CH50, C2 protein, and C2 function, and then confirmed by family studies. Analysis of HLA antigens in these families supported the close association of C2 defiency and HLA-A10 and/or B18, particularly the latter. Analysis by MLC studies revealed recombination in one family between the HLA and B and D loci and in another family probable recombination between the D and C2 complement loci. Therefore, the order of the loci on the sixth chromosome is likely to be C2 complement, HLA-D, HLA-B, HLA-A.", "contents": "Hereditary C2 deficiency: diagnosis and HLA gene complex associations. Nine families with genetically controlled C2 deficiency have been described where the propositii and family members are heterozygous C2 deficient. The diagnosis of hereditary C2 heterozygous deficiency was suspected on the basis of analysis for CH50, C2 protein, and C2 function, and then confirmed by family studies. Analysis of HLA antigens in these families supported the close association of C2 defiency and HLA-A10 and/or B18, particularly the latter. Analysis by MLC studies revealed recombination in one family between the HLA and B and D loci and in another family probable recombination between the D and C2 complement loci. Therefore, the order of the loci on the sixth chromosome is likely to be C2 complement, HLA-D, HLA-B, HLA-A."}
{"id": "PMID:1082904", "title": "Eosinophils and immune mechanisms. III. Production of the lymphokine eosinophil stimulation promoter by mouse T lymphocytes.", "content": "The lymphoid cell population responsible for production of eosinophil stimulation promoter (ESP), a lymphokine which increases migration of eosinophils, was investigated in murine Schistosoma mansoni infection. Con A challenge induced ESP production, whereas LPS did not. Prior treatment with anti-thetaC3H alloantiserum plus complement in vitro eliminated ESP production; in vivo treatment with rabbit anti-mouse thymocyte serum consistently reduced ESP production by splenic lymphoid cells, but affected lymph node cell ESP production only after exceptionally large doses. Thymocytes did not produce significant amounts of ESP; nor did lymphoid cells from congenitally athymic mice. Depletion of B lymphocytes and macrophages by nylon fiber adherence eliminated antigen-induced ESP production; this was partially restored by addition of non-immune, 72-hr peritoneal exudate cells. Con A-induced ESP production was not affected by nylon fiber treatment. These results demonstrate that ESP is  produced by an ATS-sensitive, peripheralized T lymphocyte population, and suggest a macrophage requirement for antigen-induced production of this lymphokine.", "contents": "Eosinophils and immune mechanisms. III. Production of the lymphokine eosinophil stimulation promoter by mouse T lymphocytes. The lymphoid cell population responsible for production of eosinophil stimulation promoter (ESP), a lymphokine which increases migration of eosinophils, was investigated in murine Schistosoma mansoni infection. Con A challenge induced ESP production, whereas LPS did not. Prior treatment with anti-thetaC3H alloantiserum plus complement in vitro eliminated ESP production; in vivo treatment with rabbit anti-mouse thymocyte serum consistently reduced ESP production by splenic lymphoid cells, but affected lymph node cell ESP production only after exceptionally large doses. Thymocytes did not produce significant amounts of ESP; nor did lymphoid cells from congenitally athymic mice. Depletion of B lymphocytes and macrophages by nylon fiber adherence eliminated antigen-induced ESP production; this was partially restored by addition of non-immune, 72-hr peritoneal exudate cells. Con A-induced ESP production was not affected by nylon fiber treatment. These results demonstrate that ESP is  produced by an ATS-sensitive, peripheralized T lymphocyte population, and suggest a macrophage requirement for antigen-induced production of this lymphokine."}
{"id": "PMID:1082905", "title": "Replication of measles virus in continuous lymphoid cell lines.", "content": "The replication of measles virus was studied in a T cell-like line, MOLT-4, and a B cell-like line, Raji. Both cell lines supported measles virus replication. The infected lymphoid cells were assayed for infectious virus and expression of measles antigens at timed intervals up to 84 hr after inoculation. At time 0, after a 2-hr adsorption period at 4 degrees C, both cell lines produced a maximum of 3% infectious centers and up to 22% antigen-positive cells. Eventually all cells in both populations became infected during the experimental period. When Raji and MOLT-4 cells were infected under similar conditions 100% infection of cells was achieved 12 hr earlier in Raji than in MOLT-4. Increasing multiplicity of infection (MOI) from 1 to 10 made no significant change in the number of infected cells during the early part of the cycle. Spread of virus was largely due to released virus and not cell to cell transmission. Since only a small proportion of the population absorbed the virus at any one time, and eventually all cells were infected, it was concluded that the receptors for measles virus were expressed at some phase of the cell cycle.", "contents": "Replication of measles virus in continuous lymphoid cell lines. The replication of measles virus was studied in a T cell-like line, MOLT-4, and a B cell-like line, Raji. Both cell lines supported measles virus replication. The infected lymphoid cells were assayed for infectious virus and expression of measles antigens at timed intervals up to 84 hr after inoculation. At time 0, after a 2-hr adsorption period at 4 degrees C, both cell lines produced a maximum of 3% infectious centers and up to 22% antigen-positive cells. Eventually all cells in both populations became infected during the experimental period. When Raji and MOLT-4 cells were infected under similar conditions 100% infection of cells was achieved 12 hr earlier in Raji than in MOLT-4. Increasing multiplicity of infection (MOI) from 1 to 10 made no significant change in the number of infected cells during the early part of the cycle. Spread of virus was largely due to released virus and not cell to cell transmission. Since only a small proportion of the population absorbed the virus at any one time, and eventually all cells were infected, it was concluded that the receptors for measles virus were expressed at some phase of the cell cycle."}
{"id": "PMID:1082906", "title": "The active E rosette test: correlation with delayed cutaneous hypersensitivity.", "content": "An active subpopulation of peripheral blood T lymphocytes, characterized by rapid (5-min) rosette formation with sheep erythrocytes (A-RFC), was measured in normal individuals after they were skin tested with microbial antigens. A significant rise in A-RFC occurred in all individuals who developed positive delayed cutaneous hypersensitivity (DCH) reactions, whereas skin test nonresponders showed no significant rise in A-RFC. No similar consistent changes occurred in populations of total T cells, characterized by longer (60-min) rosette formation with sheep erythrocytes, or in B cells, measured by immunofluorescence of surface immunoglobulin. The A-RFC response paralleled the DCH response in timing, but not in intensity. These results provide in vivo evidence for a biologically distinct T cell subpopulation, and focus attention on the A-RFC as immunologically active cells.", "contents": "The active E rosette test: correlation with delayed cutaneous hypersensitivity. An active subpopulation of peripheral blood T lymphocytes, characterized by rapid (5-min) rosette formation with sheep erythrocytes (A-RFC), was measured in normal individuals after they were skin tested with microbial antigens. A significant rise in A-RFC occurred in all individuals who developed positive delayed cutaneous hypersensitivity (DCH) reactions, whereas skin test nonresponders showed no significant rise in A-RFC. No similar consistent changes occurred in populations of total T cells, characterized by longer (60-min) rosette formation with sheep erythrocytes, or in B cells, measured by immunofluorescence of surface immunoglobulin. The A-RFC response paralleled the DCH response in timing, but not in intensity. These results provide in vivo evidence for a biologically distinct T cell subpopulation, and focus attention on the A-RFC as immunologically active cells."}
{"id": "PMID:1082907", "title": "Regulatory substances produced by lymphocytes. III. Evidence that lymphotoxin and proliferation inhibitory factor are identical and different from the inhibitor of DNA synthesis.", "content": "L cell mutant lines which were considerably more resistant to rat lymphotoxin (LT) than the original cell line were obtained by periodic additions of LT, partially purified from sensitized lymph node cell culture supernatants by DEAE-cellulose chromatography and Sephadex gel filtration. Addition of actinomycin D to cultures of these mutant cells abrogated resistance to LT, suqgesting that resistance was not due to a loss of LT receptors but probably to increased activity of a repair mechanisms. These mutant lines were also more resistant to the proliferation inhibitory effect of LT in low concentration and to that of diluted culture supernatants of lymph node cells stimulated with antigen (ovalbumin) than the original cell line, but they remained as sensitive to inhibitor of DNA synthesis (IDS) as the original line. The mutant lines also remained fully sensitive to both complement-dependent lysis by antibody and antibody-dependent cellular cytotoxicity, but showed increased resistance to the killing effect of rat lymph node cells sensitized with orginal L cells in vivo. These findings suggest that the lymphotoxic substance partially purified and characterized in this and in the previous paper may be an important mediator of T cell-mediated cytotoxicity.", "contents": "Regulatory substances produced by lymphocytes. III. Evidence that lymphotoxin and proliferation inhibitory factor are identical and different from the inhibitor of DNA synthesis. L cell mutant lines which were considerably more resistant to rat lymphotoxin (LT) than the original cell line were obtained by periodic additions of LT, partially purified from sensitized lymph node cell culture supernatants by DEAE-cellulose chromatography and Sephadex gel filtration. Addition of actinomycin D to cultures of these mutant cells abrogated resistance to LT, suqgesting that resistance was not due to a loss of LT receptors but probably to increased activity of a repair mechanisms. These mutant lines were also more resistant to the proliferation inhibitory effect of LT in low concentration and to that of diluted culture supernatants of lymph node cells stimulated with antigen (ovalbumin) than the original cell line, but they remained as sensitive to inhibitor of DNA synthesis (IDS) as the original line. The mutant lines also remained fully sensitive to both complement-dependent lysis by antibody and antibody-dependent cellular cytotoxicity, but showed increased resistance to the killing effect of rat lymph node cells sensitized with orginal L cells in vivo. These findings suggest that the lymphotoxic substance partially purified and characterized in this and in the previous paper may be an important mediator of T cell-mediated cytotoxicity."}
{"id": "PMID:1082908", "title": "Characterization of T and B antigen-binding cells for beta-galactosidase. II. T antigen-binding cells.", "content": "The results reported in this paper suggest that the specific Z-binding cells of the normal mouse include a large portion of T lymphocytes. Depletion of T cells with anti-theta serum and cortisone indicates that the majority of the ZBC of the thymus, which occur at frequencies of about 150/10(6), are indeed T cells. Similar treatment of spleen cells suggests that approximately half the binding cells in that organ are contributed by the T lymphocyte population. T-and B-enriched populations obtained from the spleen by using differential adherence to nylon wool contained equal numbers of ZBC and bound equivalent amounts of the antigen. Hence, there appears to be a high frequency of T lymphocytes that can be shown to bind beta-galactosidase specifically in both the thymus and spleen of normal mice.", "contents": "Characterization of T and B antigen-binding cells for beta-galactosidase. II. T antigen-binding cells. The results reported in this paper suggest that the specific Z-binding cells of the normal mouse include a large portion of T lymphocytes. Depletion of T cells with anti-theta serum and cortisone indicates that the majority of the ZBC of the thymus, which occur at frequencies of about 150/10(6), are indeed T cells. Similar treatment of spleen cells suggests that approximately half the binding cells in that organ are contributed by the T lymphocyte population. T-and B-enriched populations obtained from the spleen by using differential adherence to nylon wool contained equal numbers of ZBC and bound equivalent amounts of the antigen. Hence, there appears to be a high frequency of T lymphocytes that can be shown to bind beta-galactosidase specifically in both the thymus and spleen of normal mice."}
{"id": "PMID:1082909", "title": "Immune mechanisms in leukemia: protective capacity of the major lymphoid cell compartments.", "content": "The capacity of immune spleen, lymph node, peritoneal, bone marrow, and thymic cells to protect C58/wm mice from syngeneic transplanted line Ib leukemia was quantified. Cells harvested 14 to 15 days after primary immunization were used for adoptive protection tests. Regression curves were computer analyzed and log10, PD50 values compared. For immune spleen, lymph node, peritoneal, bone marrow, and thymic cells the PD50 values were 4.53, 5.92, 4.88, 5.51, and 5.59, respectively. When immune spleen cells were treated with anti-Thy 1.2 serum the PD50 value was increased from 4.73 to 6.09, i.e., protection was reduced greater than or equal to 95%. Similar treatment of immune thymic cells reduced protection below measureable values. Anti-B cell sera (anti-IgM and anti-Ly 4.2) did not reduce the protective effect of immune spleen or marrow cells. These results indicate that a major protective cell population in each of these compartments was theta-positive. Experiments were carried out to characterize the cortisone (CS) and x-ray sensitivity of immune spleen, thymic, and marrow cells . When donor mice were treated with 12.5 mg of cortisone acetate/day for 2 days before lymphoid cells were harvested, the orotective effects of immune spleen cells, but not immune thymic or marrow cells, was reduced. When immune spleen cells were x-irrated in vitro, their protective effect was reduced by 350 R and abolished by 1000 R. When mice received whole boyd x-irradiation 24 hr before immune spleen cells were transferred their protective effect was reduced by 1000 R but only slightly lowered by 350 R. The possible significance of the multicompartmental nature of immunity to leukemia was discussed.", "contents": "Immune mechanisms in leukemia: protective capacity of the major lymphoid cell compartments. The capacity of immune spleen, lymph node, peritoneal, bone marrow, and thymic cells to protect C58/wm mice from syngeneic transplanted line Ib leukemia was quantified. Cells harvested 14 to 15 days after primary immunization were used for adoptive protection tests. Regression curves were computer analyzed and log10, PD50 values compared. For immune spleen, lymph node, peritoneal, bone marrow, and thymic cells the PD50 values were 4.53, 5.92, 4.88, 5.51, and 5.59, respectively. When immune spleen cells were treated with anti-Thy 1.2 serum the PD50 value was increased from 4.73 to 6.09, i.e., protection was reduced greater than or equal to 95%. Similar treatment of immune thymic cells reduced protection below measureable values. Anti-B cell sera (anti-IgM and anti-Ly 4.2) did not reduce the protective effect of immune spleen or marrow cells. These results indicate that a major protective cell population in each of these compartments was theta-positive. Experiments were carried out to characterize the cortisone (CS) and x-ray sensitivity of immune spleen, thymic, and marrow cells . When donor mice were treated with 12.5 mg of cortisone acetate/day for 2 days before lymphoid cells were harvested, the orotective effects of immune spleen cells, but not immune thymic or marrow cells, was reduced. When immune spleen cells were x-irrated in vitro, their protective effect was reduced by 350 R and abolished by 1000 R. When mice received whole boyd x-irradiation 24 hr before immune spleen cells were transferred their protective effect was reduced by 1000 R but only slightly lowered by 350 R. The possible significance of the multicompartmental nature of immunity to leukemia was discussed."}
{"id": "PMID:1082910", "title": "Partial purification of lymphokine activities by iso-electric focusing.", "content": "A continuous cell-line has been shown to be adaptable to preparation of supernatants containing lymphokine activities. Separation and partial purification of migration inhibiting, colony stimulating and interferon activities from such preparations has been achieved by the use of density gradient iso-electric focusing.", "contents": "Partial purification of lymphokine activities by iso-electric focusing. A continuous cell-line has been shown to be adaptable to preparation of supernatants containing lymphokine activities. Separation and partial purification of migration inhibiting, colony stimulating and interferon activities from such preparations has been achieved by the use of density gradient iso-electric focusing."}
{"id": "PMID:1082911", "title": "Immunology of DNA. II. The effect of size and structure of the antigen on the Farr assay.", "content": "The Farr assay for the detecton of antibodies to double stranded (ds) DNA is influenced by the DNA preparations used as antigen. To elucidate this the molecular weight of the antigen preparation, contamination with proteins and presence or absence of single stranded (ss) regions were studied with the following conclusions: 1) The degree of DNA binding by antibodies is linearly dependent on the molecular weight of the DNA, provided that this does not exceed 10 X 10(6). 2) Deproteinization of E. coli DNA by chromatography on methylated albumin-kieselguhr(MAK) columns results in lower binding by most sera. 3) ds DNA preparations sometimes contain ss regions which bind antibodies to ss DNA. The difference in behaviour of different ds DNA preparations may be ascribed entirely to these factors and not to differences in antigenic determinants. We have standardized the Farr assay and enhanced its specificity by the use of circular DNA isolated from bacteriophage PM2.", "contents": "Immunology of DNA. II. The effect of size and structure of the antigen on the Farr assay. The Farr assay for the detecton of antibodies to double stranded (ds) DNA is influenced by the DNA preparations used as antigen. To elucidate this the molecular weight of the antigen preparation, contamination with proteins and presence or absence of single stranded (ss) regions were studied with the following conclusions: 1) The degree of DNA binding by antibodies is linearly dependent on the molecular weight of the DNA, provided that this does not exceed 10 X 10(6). 2) Deproteinization of E. coli DNA by chromatography on methylated albumin-kieselguhr(MAK) columns results in lower binding by most sera. 3) ds DNA preparations sometimes contain ss regions which bind antibodies to ss DNA. The difference in behaviour of different ds DNA preparations may be ascribed entirely to these factors and not to differences in antigenic determinants. We have standardized the Farr assay and enhanced its specificity by the use of circular DNA isolated from bacteriophage PM2."}
{"id": "PMID:1082914", "title": "Mechanisms of genetic resistance to Friend virus leukemia. III. Susceptibility of mitogen-responsive lymphocytes mediated by T cells.", "content": "Friend leukemia virus (FV) suppressed the proliferative responses of spleen, lymph node, marrow, and thymus cell populations to various T- and B-cell mitogens. Cells taken from mice, e.g. BALB/c genetically susceptible to leukemogenesis in vivo were much more susceptible to suppression of mitogenesis in vitro than similar cells from genetically resistant mice, e.g., C57BL/6. Nylon wool-purified splenic T cells from BALB/c and C3H mice lost susceptibility to FV-induced suppression of mitogenesis but became suppressible by addition of 10% unfiltered spleen cell. Thus, FV mediates in vitro suppression of lymphocyte proliferation indirectly by \"activating\" a suppressor cell. The suppressor cell adhered to nylon wool but not to glass wool or rayon wool columns. Pretreatment of spleen cells with carbonyl iron and a magnet did not abrogate the suppressor cell function. Suppressor cells were not eliminated by treatment with rabbit antimouse immunoglobulin (7S) and complement (C). However, high concentrations of anti-Thy-1 plus C destroyed suppressor cells of the spleen; thymic suppressor cells were much more susceptible to anti-Thy-1 serum. Nude athymic mice were devoid of suppressor cells and their B-cell proliferation was relatively resistant to FV-induced suppression in vitro. The suppressor cells in the thymus (but not in the spleen) were eliminated by treatment of mice with cortisol. Thus, FV appears to mediate its suppressive effect on mitogen-responsive lymphocytes by affecting \"T-suppressor cells.\" Spleen cells from C57BL/6 mice treated with 89Sr to destroy marrow-dependent (M) cells were much more suppressible by FV in virto than normal C57BL/6 spleen cells. However, nylon-filtered spleen cells of 89Sr-treated C57BL/6 mice were resistant to FV-induced suppression in vitro, indicating that the susceptibility of spleen cells from 89Sr-treated B6 mice is also mediated by suppressor cells. Normal B6 splenic T cells were rendered susceptible to FV-induced suppression of mitogenesis by addition of 10% spleen cells from 89Sr-treated B6 mice. Thus, M cells appear to regulate the numbers and/or functions of T-suppressor cells which in turn mediate the immunosuppressive effects of FV in vitro. Neither mitogen-responsive lymphocytes nor T-suppressor cells are genetically resistant or susceptible to FV. The genetic resistance to FV is apparently a function of M cells, both in vitro as well as in vivo.", "contents": "Mechanisms of genetic resistance to Friend virus leukemia. III. Susceptibility of mitogen-responsive lymphocytes mediated by T cells. Friend leukemia virus (FV) suppressed the proliferative responses of spleen, lymph node, marrow, and thymus cell populations to various T- and B-cell mitogens. Cells taken from mice, e.g. BALB/c genetically susceptible to leukemogenesis in vivo were much more susceptible to suppression of mitogenesis in vitro than similar cells from genetically resistant mice, e.g., C57BL/6. Nylon wool-purified splenic T cells from BALB/c and C3H mice lost susceptibility to FV-induced suppression of mitogenesis but became suppressible by addition of 10% unfiltered spleen cell. Thus, FV mediates in vitro suppression of lymphocyte proliferation indirectly by \"activating\" a suppressor cell. The suppressor cell adhered to nylon wool but not to glass wool or rayon wool columns. Pretreatment of spleen cells with carbonyl iron and a magnet did not abrogate the suppressor cell function. Suppressor cells were not eliminated by treatment with rabbit antimouse immunoglobulin (7S) and complement (C). However, high concentrations of anti-Thy-1 plus C destroyed suppressor cells of the spleen; thymic suppressor cells were much more susceptible to anti-Thy-1 serum. Nude athymic mice were devoid of suppressor cells and their B-cell proliferation was relatively resistant to FV-induced suppression in vitro. The suppressor cells in the thymus (but not in the spleen) were eliminated by treatment of mice with cortisol. Thus, FV appears to mediate its suppressive effect on mitogen-responsive lymphocytes by affecting \"T-suppressor cells.\" Spleen cells from C57BL/6 mice treated with 89Sr to destroy marrow-dependent (M) cells were much more suppressible by FV in virto than normal C57BL/6 spleen cells. However, nylon-filtered spleen cells of 89Sr-treated C57BL/6 mice were resistant to FV-induced suppression in vitro, indicating that the susceptibility of spleen cells from 89Sr-treated B6 mice is also mediated by suppressor cells. Normal B6 splenic T cells were rendered susceptible to FV-induced suppression of mitogenesis by addition of 10% spleen cells from 89Sr-treated B6 mice. Thus, M cells appear to regulate the numbers and/or functions of T-suppressor cells which in turn mediate the immunosuppressive effects of FV in vitro. Neither mitogen-responsive lymphocytes nor T-suppressor cells are genetically resistant or susceptible to FV. The genetic resistance to FV is apparently a function of M cells, both in vitro as well as in vivo."}
{"id": "PMID:1082915", "title": "Killer cells: a functional comparison between natural, immune T-cell and antibody-dependent in vitro systems.", "content": "Previous reports have shown that spleen cells from nonimmune adult mice of certain strains do regularly kill Moloney leukemia virus-induced lymphomas in short-term 51Cr release assays. This naturally occuring killer (NK) cell had low adherent properties and had the morphological appearance of a lymphocyte. Still it lacked surface characteristics of mature T or B lymphocytes. In the present report a functional study was carried out, comparing in parallel the NK system, the T-cell killing across an H-2 barrier (anti-P815), and the antibody-dependent cell-mediated chicken red blood cell (CRBC) system. In contrast to the effector cells in the CRBC system, the NK cells were insensitive to erythrocyte antibody complement (EAC) rosette depletion and would pass through nylon wool columns. NK activity was not inhibited by the presence of heat-aggregated human or mouse gamma globulin, in contrast to the strong inhibition noted in the CRBC system. Sensitivity to trypsin pretreatment was noted in the NK system as well as in the immune P815 system, whereas the CRBC system was relatively trypsin resistant. Antitheta plus complement eliminated the anti-P815 activity, but did not touch the NK activity. The present results thus further distinguish the NK cell from cytotoxic T lymphocytes or from antibody-dependent killer cells.", "contents": "Killer cells: a functional comparison between natural, immune T-cell and antibody-dependent in vitro systems. Previous reports have shown that spleen cells from nonimmune adult mice of certain strains do regularly kill Moloney leukemia virus-induced lymphomas in short-term 51Cr release assays. This naturally occuring killer (NK) cell had low adherent properties and had the morphological appearance of a lymphocyte. Still it lacked surface characteristics of mature T or B lymphocytes. In the present report a functional study was carried out, comparing in parallel the NK system, the T-cell killing across an H-2 barrier (anti-P815), and the antibody-dependent cell-mediated chicken red blood cell (CRBC) system. In contrast to the effector cells in the CRBC system, the NK cells were insensitive to erythrocyte antibody complement (EAC) rosette depletion and would pass through nylon wool columns. NK activity was not inhibited by the presence of heat-aggregated human or mouse gamma globulin, in contrast to the strong inhibition noted in the CRBC system. Sensitivity to trypsin pretreatment was noted in the NK system as well as in the immune P815 system, whereas the CRBC system was relatively trypsin resistant. Antitheta plus complement eliminated the anti-P815 activity, but did not touch the NK activity. The present results thus further distinguish the NK cell from cytotoxic T lymphocytes or from antibody-dependent killer cells."}
{"id": "PMID:1082916", "title": "The effect of anti-mu suppression of gammaM and gammaG on the production of gammaE.", "content": "Newbonr mice were treated from the day of birth with either bovine gamma globulin or anti-mu chain sera. The latter was administered using a protocol known to produce suppression of gammaM, gammaG, and gammaA production. Subsequent immunization with ovalbumin (OA) in alum was attempted to see if suppression of gammaM, gammaG, and gammaA classes of antibody would also be accompanied by suppression of gammaE-producing capacity. gammaG and gammaM antibody to OA and mercaptoethanol-resistant (gammaG) antibody to OA were measured by passive hemagglutination; gammaG and gammaE anti-OA antibodies were measured by passive cutaneous anaphylaxis. Anti-mu suppression was achieved with significant reduction in gammaM and gammaG antibodies. gammaE antibodies were not affected, suggesting an ontogenetic development for gammaE-bearing lymphocytes independent of the previously described gammaM to gammaG to gammaA ontogenetic sequence.", "contents": "The effect of anti-mu suppression of gammaM and gammaG on the production of gammaE. Newbonr mice were treated from the day of birth with either bovine gamma globulin or anti-mu chain sera. The latter was administered using a protocol known to produce suppression of gammaM, gammaG, and gammaA production. Subsequent immunization with ovalbumin (OA) in alum was attempted to see if suppression of gammaM, gammaG, and gammaA classes of antibody would also be accompanied by suppression of gammaE-producing capacity. gammaG and gammaM antibody to OA and mercaptoethanol-resistant (gammaG) antibody to OA were measured by passive hemagglutination; gammaG and gammaE anti-OA antibodies were measured by passive cutaneous anaphylaxis. Anti-mu suppression was achieved with significant reduction in gammaM and gammaG antibodies. gammaE antibodies were not affected, suggesting an ontogenetic development for gammaE-bearing lymphocytes independent of the previously described gammaM to gammaG to gammaA ontogenetic sequence."}
{"id": "PMID:1082917", "title": "Antigen recognition. IV. Discrimination by antigen-binding immunocompetent B cells between immunity and tolerance is determined by adherent cells.", "content": "Mouse spleen cells capable of specifically binding intrinsically tritium-labeled polymerized flagellin (POL) (labeling by biosynthesis of flagellar protein) via IgM receptors were found to comprise a distinct population of about 20-50 cells per 10(6) lymphocytes. Evidence is presented that the majority of mouse spleen cells binding tritium-labeled POL undergoes blastogenesis after antigen capping, antigen shedding, and receptor reformation. Under conditions of tolerance induction in vitro, however, loss of antigen from the cell surface was inhibited. Such inhibition of antigen redistribution and shedding was reversed by a short pulse of colchicine and new antigen receptors were formed. In spite of this, colchicine had no effect on the tolerant state. However, tolerance could be broken, regardless of presence or absence of the alkaloid, with radioresistant theta-negative accessory (A) cells (adherent cells) from normal but not from tolerant spleen cell populations. \"Tolerant\" A cells, although they were incapable of cooperating in a response to POL, were capable of participating in a response to a second unrelated antigen. It is concluded that tolerance to POL in vitro is induced by mechanisms other than the physical blocking of bone marrow-derived (B) cell receptors by antigen. Most likely, the discrimination by the B cell between a tolerogenic and immunogenic signal is mediated by A cells.", "contents": "Antigen recognition. IV. Discrimination by antigen-binding immunocompetent B cells between immunity and tolerance is determined by adherent cells. Mouse spleen cells capable of specifically binding intrinsically tritium-labeled polymerized flagellin (POL) (labeling by biosynthesis of flagellar protein) via IgM receptors were found to comprise a distinct population of about 20-50 cells per 10(6) lymphocytes. Evidence is presented that the majority of mouse spleen cells binding tritium-labeled POL undergoes blastogenesis after antigen capping, antigen shedding, and receptor reformation. Under conditions of tolerance induction in vitro, however, loss of antigen from the cell surface was inhibited. Such inhibition of antigen redistribution and shedding was reversed by a short pulse of colchicine and new antigen receptors were formed. In spite of this, colchicine had no effect on the tolerant state. However, tolerance could be broken, regardless of presence or absence of the alkaloid, with radioresistant theta-negative accessory (A) cells (adherent cells) from normal but not from tolerant spleen cell populations. \"Tolerant\" A cells, although they were incapable of cooperating in a response to POL, were capable of participating in a response to a second unrelated antigen. It is concluded that tolerance to POL in vitro is induced by mechanisms other than the physical blocking of bone marrow-derived (B) cell receptors by antigen. Most likely, the discrimination by the B cell between a tolerogenic and immunogenic signal is mediated by A cells."}
{"id": "PMID:1082918", "title": "Suppression of IgE antibody production in SJL mice. I. Nonspecific suppressor T cells.", "content": "High titer and persistent antihapten IgE production in SJL mice can be obtained using appropriate immunization and radiation. Nonirradiated mice rapidly terminate this antihapten IgE production. Radiation was not necessary to prolong antihapten IgE production in other strains of mice. Termination can be obtained even in irradiated SJL mice by transferring normal SJL spleen cells. That the suppressor cells are T cells is shown by using thymocytes or cells treated with anti-Thy 1.2 and complement. No appreciable suppressive effect by normal spleen cells could be demonstrated on IgG1 production in SJL mice. The characteristic of low and transient IgE antibody response in SJL mice is inherited as a recessive trait controlled by a single Mendelian autosomal gene and is not linked to the H-2-gene complex. This characteristic does not depend on the infectivity of Nippostrongylus brasiliensis, the effect of anticarrier antibody, or the recognition of antigen.", "contents": "Suppression of IgE antibody production in SJL mice. I. Nonspecific suppressor T cells. High titer and persistent antihapten IgE production in SJL mice can be obtained using appropriate immunization and radiation. Nonirradiated mice rapidly terminate this antihapten IgE production. Radiation was not necessary to prolong antihapten IgE production in other strains of mice. Termination can be obtained even in irradiated SJL mice by transferring normal SJL spleen cells. That the suppressor cells are T cells is shown by using thymocytes or cells treated with anti-Thy 1.2 and complement. No appreciable suppressive effect by normal spleen cells could be demonstrated on IgG1 production in SJL mice. The characteristic of low and transient IgE antibody response in SJL mice is inherited as a recessive trait controlled by a single Mendelian autosomal gene and is not linked to the H-2-gene complex. This characteristic does not depend on the infectivity of Nippostrongylus brasiliensis, the effect of anticarrier antibody, or the recognition of antigen."}
{"id": "PMID:1082919", "title": "The requirement for two complementing Ir-GLphi immune response genes in the T-lymphocyte proliferative response to poly-(Glu53Lys36Phe11).", "content": "The antibody response to poly-(Glu53Lys36Phe11) (GLphi) has been shown to be under the control of two independent, major histocompatibility-linked immune response genes, designated alpha and beta. In the present work we demonstrate that the T-lymphocyte proliferative response is also under the control of these two immune response genes. Thus, mice of the H-2a, H-2b, H-2k, and H-2s haplotypes were all nonresponders to GLphi. In contrast F1 hybrids between these strains, such as (B10 X B10.A)F1 and (C3H X SJL)F1, as well as several recombinant mice derived from the nonresponder haplotypes, such as B10.1(5R), B10.HTT, and B10.S(9R), were all responders to GLphi. The complementation between nonresponder genomes appeared to be stronger in the cis position than in the trans position for some strain combinations. The failure of strains bearing only one of the two responder alleles to show a T-lymphocyte proliferative response to GLphi, argues strongly that neither gene can be expressed exclusively in B lymphocytes. This conclusion is discussed in relation to another two gene model which has recently been proposed.", "contents": "The requirement for two complementing Ir-GLphi immune response genes in the T-lymphocyte proliferative response to poly-(Glu53Lys36Phe11). The antibody response to poly-(Glu53Lys36Phe11) (GLphi) has been shown to be under the control of two independent, major histocompatibility-linked immune response genes, designated alpha and beta. In the present work we demonstrate that the T-lymphocyte proliferative response is also under the control of these two immune response genes. Thus, mice of the H-2a, H-2b, H-2k, and H-2s haplotypes were all nonresponders to GLphi. In contrast F1 hybrids between these strains, such as (B10 X B10.A)F1 and (C3H X SJL)F1, as well as several recombinant mice derived from the nonresponder haplotypes, such as B10.1(5R), B10.HTT, and B10.S(9R), were all responders to GLphi. The complementation between nonresponder genomes appeared to be stronger in the cis position than in the trans position for some strain combinations. The failure of strains bearing only one of the two responder alleles to show a T-lymphocyte proliferative response to GLphi, argues strongly that neither gene can be expressed exclusively in B lymphocytes. This conclusion is discussed in relation to another two gene model which has recently been proposed."}
{"id": "PMID:1082920", "title": "Control of t-lymphocyte and B-lymphocyte activation by two complementing Ir-GLphi immune response genes.", "content": "The possibility that the two complementing alpha- and beta-Ir-GLphi genes are independently responsible for controlling events in T lymphocytes and B lymphocytes, respectively, has been tested in double adoptive transfer experiments utilizing cells from appropriate inbred strains of mice. The results of these studies show that the functions of T lymphocytes and B lymphocytes and the cooperative interactions between T and B cells require the presence of both alpha- and beta-genes in each respective cell type. Moreover, evidence has been obtained in these studies that indicates a preference for the alpha- and beta-Ir-GLphi genes in the cis position to obtain the most effective T-B-cell interactions. The possible implications of these findings are discussed.", "contents": "Control of t-lymphocyte and B-lymphocyte activation by two complementing Ir-GLphi immune response genes. The possibility that the two complementing alpha- and beta-Ir-GLphi genes are independently responsible for controlling events in T lymphocytes and B lymphocytes, respectively, has been tested in double adoptive transfer experiments utilizing cells from appropriate inbred strains of mice. The results of these studies show that the functions of T lymphocytes and B lymphocytes and the cooperative interactions between T and B cells require the presence of both alpha- and beta-genes in each respective cell type. Moreover, evidence has been obtained in these studies that indicates a preference for the alpha- and beta-Ir-GLphi genes in the cis position to obtain the most effective T-B-cell interactions. The possible implications of these findings are discussed."}
{"id": "PMID:1082921", "title": "In vitro studies of the rabbit immune system. V. Suppressor T cells activated by concanavalin A block the proliferation, not the induction of antierythrocyte plaque-forming cells.", "content": "The late B-cell proliferative phase of the in vitro antibody response by rabbit spleen cells is highly susceptible to suppression by activated T cells. The in vitro antisheep erythrocyte plaque-forming cell (PFC) response by spleen cells from normal or primed rabbits can be suppressed by adding concanavalin A (Con A), Con A-prestimulated peripheral blood or spleen lymphocytes, or supernates from Con A-prestimulated peripheral blood lymphocytes. The suppression is not mediated by a direct interaction of Con A with responding cells as shown by the effectiveness of prestimulated cells. Primed spleen cultures remain sensitive to Con A suppression as late as 72 h after initiation, and the addition of Con A after 24-72 h rapidly stops the increase in the number of PFC. T cells are required for Con A addition to be effective but the suppression can be induced at a time when T-helper cells are no longer necessary. Further, the suppressive effect of Con A addition is abrogated by specific antisera to rabbit T cells. We propose that Con A activates suppressor T cells which then exert their effects on proliferating PFC or their immediate precursor B cells. The early inductive or recruitment phase of the response is probably not blocked by suppressor cells. Also, there is an apparent relationship between the number of proliferating B cells and the number of suppressor cells required. Finally, the difficulties in inducing a stimulatory effect by Con A and the prolonged period that Con A addition is suppressive suggests that the rabbit has relatively more and/or longer-lived suppressor cells than the mouse and may be a particularly useful species for studying suppressive phenomena and their mechanisms.", "contents": "In vitro studies of the rabbit immune system. V. Suppressor T cells activated by concanavalin A block the proliferation, not the induction of antierythrocyte plaque-forming cells. The late B-cell proliferative phase of the in vitro antibody response by rabbit spleen cells is highly susceptible to suppression by activated T cells. The in vitro antisheep erythrocyte plaque-forming cell (PFC) response by spleen cells from normal or primed rabbits can be suppressed by adding concanavalin A (Con A), Con A-prestimulated peripheral blood or spleen lymphocytes, or supernates from Con A-prestimulated peripheral blood lymphocytes. The suppression is not mediated by a direct interaction of Con A with responding cells as shown by the effectiveness of prestimulated cells. Primed spleen cultures remain sensitive to Con A suppression as late as 72 h after initiation, and the addition of Con A after 24-72 h rapidly stops the increase in the number of PFC. T cells are required for Con A addition to be effective but the suppression can be induced at a time when T-helper cells are no longer necessary. Further, the suppressive effect of Con A addition is abrogated by specific antisera to rabbit T cells. We propose that Con A activates suppressor T cells which then exert their effects on proliferating PFC or their immediate precursor B cells. The early inductive or recruitment phase of the response is probably not blocked by suppressor cells. Also, there is an apparent relationship between the number of proliferating B cells and the number of suppressor cells required. Finally, the difficulties in inducing a stimulatory effect by Con A and the prolonged period that Con A addition is suppressive suggests that the rabbit has relatively more and/or longer-lived suppressor cells than the mouse and may be a particularly useful species for studying suppressive phenomena and their mechanisms."}
{"id": "PMID:1082922", "title": "B-lymphocyte alloantigens in Caucasians.", "content": "Human B lymphocytes have been shown to have at least five polymorphic specificities defined by 32 antisera. The antisera were produced by absorption with pooled platelets to remove HLA activity and were selected out of over 400 tested sera. The sera that defined the five specificities had high correlation coefficients within a group (generally in the range of 0.6-0.9). As shown by the fit in the Hardy-Weinberg equilibrium, the five specificities appear to be determined by alleles at one genetic locus. No association between these specificities and HLA was noted.", "contents": "B-lymphocyte alloantigens in Caucasians. Human B lymphocytes have been shown to have at least five polymorphic specificities defined by 32 antisera. The antisera were produced by absorption with pooled platelets to remove HLA activity and were selected out of over 400 tested sera. The sera that defined the five specificities had high correlation coefficients within a group (generally in the range of 0.6-0.9). As shown by the fit in the Hardy-Weinberg equilibrium, the five specificities appear to be determined by alleles at one genetic locus. No association between these specificities and HLA was noted."}
{"id": "PMID:1082923", "title": "Virus and trinitrophenol hapten-specific T-cell-mediated cytotoxicity against H-2 incompatible target cells.", "content": "Immune spleen cells from LCM virus-infected (CBA X C57BL/6)F1 radiation chimeras entirely repopulated with CBA-T6 lymphocytes were cytotoxic for allogeneic, LCM virus infected C57BL/6 mouse-derived target cells. Normal C57BL/6 targets were not lysed. CBA-T6 lymphocytes derived from (CBA X C57BL/6) radiation chimeras sensitized in vitro against TNP-conjugated C57BL/6 spleen cells lysed TNP-conjugated C57BL/6 targets. However normal C57BL/6 mouse-derived targets were not destroyed. The magnitude of virus-specific (or TNP-specific) cytotoxic responses against H-2 incompatible targets was lower compared to that against H-2 compatible targets. These data are considered to support and to extend the altered self concept, but are not consistent with the dual recognition concept.", "contents": "Virus and trinitrophenol hapten-specific T-cell-mediated cytotoxicity against H-2 incompatible target cells. Immune spleen cells from LCM virus-infected (CBA X C57BL/6)F1 radiation chimeras entirely repopulated with CBA-T6 lymphocytes were cytotoxic for allogeneic, LCM virus infected C57BL/6 mouse-derived target cells. Normal C57BL/6 targets were not lysed. CBA-T6 lymphocytes derived from (CBA X C57BL/6) radiation chimeras sensitized in vitro against TNP-conjugated C57BL/6 spleen cells lysed TNP-conjugated C57BL/6 targets. However normal C57BL/6 mouse-derived targets were not destroyed. The magnitude of virus-specific (or TNP-specific) cytotoxic responses against H-2 incompatible targets was lower compared to that against H-2 compatible targets. These data are considered to support and to extend the altered self concept, but are not consistent with the dual recognition concept."}
{"id": "PMID:1082924", "title": "Effects of membrane potential on the capacitance of skeletal muscle fibers.", "content": "A method for measuring muscle fiber capacitance using small test pulses applied with the three-microelectrode voltage clamp is presented. Using this method, three membrane potential-dependent changes in capacitance were observed: (a) Capacitance of polarized fibers increased by 5--15% with depolarization from V less then -100 mV to voltages slightly below the contraction threshold. (b) Capacitance of fibers depolarized to -30 mV by 100 mM Rb solution decreased by roughly 8% with further depolarization to about +50 mV and increased with repolarization, exhibiting a maximum increase of about 10% at -80 to -90 mV. (c) Capacitance of fibers depolarized to -15 mV by 100 mM K solution increased by about 19% with further depolarization to +43 mV and decreased by about 23% with repolarization to -62 mV. Effects a and b are attributed to changes in specific membrane capacitance due to voltage-dependent redistribution of mobile charged groups within surface of T-tubule membranes. Effect c is caused by changes in the T-system space constant lambdaT due to the voltage dependence of K conductance (inward rectification). Analysis of c showed that in 100 mM K solution lambdaT congruent to 30 mum when inward rectification was fully activated by hyperpolarization and that the density of inward rectifier channels is about the same in surface and tubular membranes. Fiber internal resistance was found to be independent of voltage, a necessary condition for the interpretation of the capacitance measurements.", "contents": "Effects of membrane potential on the capacitance of skeletal muscle fibers. A method for measuring muscle fiber capacitance using small test pulses applied with the three-microelectrode voltage clamp is presented. Using this method, three membrane potential-dependent changes in capacitance were observed: (a) Capacitance of polarized fibers increased by 5--15% with depolarization from V less then -100 mV to voltages slightly below the contraction threshold. (b) Capacitance of fibers depolarized to -30 mV by 100 mM Rb solution decreased by roughly 8% with further depolarization to about +50 mV and increased with repolarization, exhibiting a maximum increase of about 10% at -80 to -90 mV. (c) Capacitance of fibers depolarized to -15 mV by 100 mM K solution increased by about 19% with further depolarization to +43 mV and decreased by about 23% with repolarization to -62 mV. Effects a and b are attributed to changes in specific membrane capacitance due to voltage-dependent redistribution of mobile charged groups within surface of T-tubule membranes. Effect c is caused by changes in the T-system space constant lambdaT due to the voltage dependence of K conductance (inward rectification). Analysis of c showed that in 100 mM K solution lambdaT congruent to 30 mum when inward rectification was fully activated by hyperpolarization and that the density of inward rectifier channels is about the same in surface and tubular membranes. Fiber internal resistance was found to be independent of voltage, a necessary condition for the interpretation of the capacitance measurements."}
{"id": "PMID:1082928", "title": "Computer mapping of brain-stem sensory centers in man.", "content": "A computer program is described for use on-line with a two--stage stereotaxic technique in man. It has four capabilities. It transposes coordinates between a brain atlas and a stereotaxic frame, simultaneously adjusting for variations in individual brain dimensions. Corrections for electrode trajectories, and for any irregularities of placement of the stereotaxic frame in the head are also made. It plots an operative template that shows electrode trajectories and the sites from which physiological data are collected appropriately oriented upon a sagittal diagram of the brain. It graphically displays physiological data in the form of a Woolsey-type figurine chart that indicates threshold in milliamperes and, through the use of coded symbols, the quality of response and certain other data. It stores all this data in a tape library together with the locations of any lesions made. This makes it possible to replot any individual patient's operative data at any time. It also permits scanning of the stored protocols from all patients for any particular type of data either by itself or in combination with other data. This retrieved information is plotted on appropriate sagittal brain diagrams corrected for individual variations in brain size. Illustrations of the capabilities of the program are presented particularly with reference to the primary afferent pathways of the upper brain stem.", "contents": "Computer mapping of brain-stem sensory centers in man. A computer program is described for use on-line with a two--stage stereotaxic technique in man. It has four capabilities. It transposes coordinates between a brain atlas and a stereotaxic frame, simultaneously adjusting for variations in individual brain dimensions. Corrections for electrode trajectories, and for any irregularities of placement of the stereotaxic frame in the head are also made. It plots an operative template that shows electrode trajectories and the sites from which physiological data are collected appropriately oriented upon a sagittal diagram of the brain. It graphically displays physiological data in the form of a Woolsey-type figurine chart that indicates threshold in milliamperes and, through the use of coded symbols, the quality of response and certain other data. It stores all this data in a tape library together with the locations of any lesions made. This makes it possible to replot any individual patient's operative data at any time. It also permits scanning of the stored protocols from all patients for any particular type of data either by itself or in combination with other data. This retrieved information is plotted on appropriate sagittal brain diagrams corrected for individual variations in brain size. Illustrations of the capabilities of the program are presented particularly with reference to the primary afferent pathways of the upper brain stem."}
{"id": "PMID:1082931", "title": "Otitis media caused by non-typable, ampicillin-resistant strains of Haemophilus influenzae.", "content": "Strains of Haemophilus influenzae, which did not belong to any of the known capsular serotypes and which were resistant in vitro to ampicillin, have been isolated from middle ear exudates of two children with otitis media. These children failed to improve with ampicillin therapy, and the resistant organisms persisted at the site of infection. Patients infected with such ampicillin-resistant organisms require therapy with antimicrobial agents which are not susceptible to degradation by penicillinase.", "contents": "Otitis media caused by non-typable, ampicillin-resistant strains of Haemophilus influenzae. Strains of Haemophilus influenzae, which did not belong to any of the known capsular serotypes and which were resistant in vitro to ampicillin, have been isolated from middle ear exudates of two children with otitis media. These children failed to improve with ampicillin therapy, and the resistant organisms persisted at the site of infection. Patients infected with such ampicillin-resistant organisms require therapy with antimicrobial agents which are not susceptible to degradation by penicillinase."}
{"id": "PMID:1082932", "title": "An analysis of the action of atropine and scopolamine on the end-plate current of frog sartorius muscle.", "content": "The effects of atropine sulfate and scopolamine hydrobromide were investigated on the end-plate current (EPC) of frog sartorius muscle by standard voltage-clamp techniques. Both atropine and scopolamine reduced the peak EPC amplitude although scopolamine was only one-third as potent as atropine. The reduction of amplitude became more pronounced with increasing membrane hyperpolarization resulting in nonlinear current-voltage characteristics. Atropine shortened the EPC duration and decreased the voltage-sensitivity of the falling phase; the latter, however, continued to remain a single exponential function of time as in the control. Scopolamine reduced the time to peak, and in addition, converted the falling phase to a double exponential function, consisting of a rapid initial phase followed by a slow terminal phase. Both phases of fall were altered by changes in drug concentration but only the terminal phase responded appreciably to changes in membrane potential. Atropine and scopolamine were without effect on the EPC reversal potential, indicating that the drugs do not exhibit a preference for the ionic species carrying the synaptic current.", "contents": "An analysis of the action of atropine and scopolamine on the end-plate current of frog sartorius muscle. The effects of atropine sulfate and scopolamine hydrobromide were investigated on the end-plate current (EPC) of frog sartorius muscle by standard voltage-clamp techniques. Both atropine and scopolamine reduced the peak EPC amplitude although scopolamine was only one-third as potent as atropine. The reduction of amplitude became more pronounced with increasing membrane hyperpolarization resulting in nonlinear current-voltage characteristics. Atropine shortened the EPC duration and decreased the voltage-sensitivity of the falling phase; the latter, however, continued to remain a single exponential function of time as in the control. Scopolamine reduced the time to peak, and in addition, converted the falling phase to a double exponential function, consisting of a rapid initial phase followed by a slow terminal phase. Both phases of fall were altered by changes in drug concentration but only the terminal phase responded appreciably to changes in membrane potential. Atropine and scopolamine were without effect on the EPC reversal potential, indicating that the drugs do not exhibit a preference for the ionic species carrying the synaptic current."}
{"id": "PMID:1082933", "title": "Aerobic recovery metabolism following a single isometric tetanus in frog sartorius muscle at 0 degrees C.", "content": "1. Basal and recovery O2 consumption, delatO2, in frog sartorius muscles at 0 degrees C were measured with a polarographic electrode. Reproducible observations were made with the same muscle over many hours. 2. The experimental records had an exponential form except for the early phases of recovery following a single isometric tetanus. Diffusion of O2 within the muscle was adequate to account for this deviation from an exponential time course of recovery. The time constant of the recovery O2 consumption increased with the duration of tetanic stimulation from 5 to 20 sec. 3. Lactate synthesis was measureable in unstimulated aerobic muscles and increased in proportion to total O2 consumption as long as the muscle did not lack O2. The contribution of glycolysis to the total chemical energy production during recovery was 6-9%; for hypoxic muscles it was greater. 4. The resynthesis of phosphorylcreatine and the decrease in inorganic phosphate and free creatine following a tetanus showed an exponential time course similar to recovery O2. Initial concentrations were re-attained within 60 min following a 20 sec tetanus. 5. We conclude that recovery O2 consumpation is a useful and accurate measure of the net chemical energy utilization for a single contraction.", "contents": "Aerobic recovery metabolism following a single isometric tetanus in frog sartorius muscle at 0 degrees C. 1. Basal and recovery O2 consumption, delatO2, in frog sartorius muscles at 0 degrees C were measured with a polarographic electrode. Reproducible observations were made with the same muscle over many hours. 2. The experimental records had an exponential form except for the early phases of recovery following a single isometric tetanus. Diffusion of O2 within the muscle was adequate to account for this deviation from an exponential time course of recovery. The time constant of the recovery O2 consumption increased with the duration of tetanic stimulation from 5 to 20 sec. 3. Lactate synthesis was measureable in unstimulated aerobic muscles and increased in proportion to total O2 consumption as long as the muscle did not lack O2. The contribution of glycolysis to the total chemical energy production during recovery was 6-9%; for hypoxic muscles it was greater. 4. The resynthesis of phosphorylcreatine and the decrease in inorganic phosphate and free creatine following a tetanus showed an exponential time course similar to recovery O2. Initial concentrations were re-attained within 60 min following a 20 sec tetanus. 5. We conclude that recovery O2 consumpation is a useful and accurate measure of the net chemical energy utilization for a single contraction."}
{"id": "PMID:1082934", "title": "Relationship between initial chemical reactions and oxidative recovery metabolism for single isometric contractions of frog sartorius at 0 degrees C.", "content": "1. Measurements of intitial chemical changes and recovery oxygen consumption (deltaO2) were made in unpoisoned aerobic frog sartorius muscles at 0 degrees C to provide independent measures of the chemical energy used for isometric tetani of various durations. 2. DeltaO2 was measured polarographically and increased in a curvilinear fashion with stimulus duration. For stimulations longer than 4 sec deltaO2 was a linear function of the tension-time integral. 3. Measurements of the changes in the content of phosphorylcreatine, 'free' creatine and inorganic phosphate were made in muscles rapidly frozen during a tetanus. The average of these quantities, delta approximately P, was used to measure the initial 'high energy' phosphate utilization. No break-down of 'high-energy' phosphate compounds was detected up to 200 sec after relaxation of tension. Changes in the content of ATP were not observed except for a small decrease (-0-25+/-0-1 mumole/g) in muscles tetanized for 1 sec. 4. Delta approximately P also increase curvilinearly with tetanus duration and, for tetanic durations greater than 4 sec, was a linear function of the tension-time integral. 5. Both deltaO2 and delta approximately P were quantitatively related by a constant scaling factor of about 4-3 (delta approximately P/deltaO2) throughout the range of tetanic durations studied. The constancy of this ratio.provides evidence against the hypothesis that a significant 'missing reaction' provides energy during any one portion of the tetanus. Several hypothesis may account for the numerical value of the ration delta approximately P/deltaO2.", "contents": "Relationship between initial chemical reactions and oxidative recovery metabolism for single isometric contractions of frog sartorius at 0 degrees C. 1. Measurements of intitial chemical changes and recovery oxygen consumption (deltaO2) were made in unpoisoned aerobic frog sartorius muscles at 0 degrees C to provide independent measures of the chemical energy used for isometric tetani of various durations. 2. DeltaO2 was measured polarographically and increased in a curvilinear fashion with stimulus duration. For stimulations longer than 4 sec deltaO2 was a linear function of the tension-time integral. 3. Measurements of the changes in the content of phosphorylcreatine, 'free' creatine and inorganic phosphate were made in muscles rapidly frozen during a tetanus. The average of these quantities, delta approximately P, was used to measure the initial 'high energy' phosphate utilization. No break-down of 'high-energy' phosphate compounds was detected up to 200 sec after relaxation of tension. Changes in the content of ATP were not observed except for a small decrease (-0-25+/-0-1 mumole/g) in muscles tetanized for 1 sec. 4. Delta approximately P also increase curvilinearly with tetanus duration and, for tetanic durations greater than 4 sec, was a linear function of the tension-time integral. 5. Both deltaO2 and delta approximately P were quantitatively related by a constant scaling factor of about 4-3 (delta approximately P/deltaO2) throughout the range of tetanic durations studied. The constancy of this ratio.provides evidence against the hypothesis that a significant 'missing reaction' provides energy during any one portion of the tetanus. Several hypothesis may account for the numerical value of the ration delta approximately P/deltaO2."}
{"id": "PMID:1082936", "title": "[Vertebral angiography and tumours of the posterior fossa in children. 29 cases (author's transl)].", "content": "With reference to 29 cases of tumours of the posterior fossa, the authors attempt to place the value of vertebral angiography amongst other diagnostic techniques and in particular in relation to ventriculography using iodised hydrosoluble contrast media. Whilst recognising the diagnostic possibilities offered by vertebral angiography, they feel that, in many cases, opacification of the IVth ventricle is necessary and that angiography does not therefore form part of the routine pre-operative assessment of such tumours.", "contents": "[Vertebral angiography and tumours of the posterior fossa in children. 29 cases (author's transl)]. With reference to 29 cases of tumours of the posterior fossa, the authors attempt to place the value of vertebral angiography amongst other diagnostic techniques and in particular in relation to ventriculography using iodised hydrosoluble contrast media. Whilst recognising the diagnostic possibilities offered by vertebral angiography, they feel that, in many cases, opacification of the IVth ventricle is necessary and that angiography does not therefore form part of the routine pre-operative assessment of such tumours."}
{"id": "PMID:1082935", "title": "The effect of caffeine and tetracaine on the time course of potassium contractures of single muscle fibres.", "content": "1. The time course of potassium contractures can be significantly prolonged by low concentrations of caffeine. 2. This effect of caffeine is not due to impairment of the fibre relaxing system. 3. Under conditions were contractile repriming is delayed (low temperature) an extra amount of activator can be released by caffeine, in addition to that released by potassium. 4. The source of this extra amount of activator is intracellular since its release can be shown in a O calcium EGTA medium. 5. Local anaesthetics, tetracaine, and to a lesser extent procaine, affect the release of contractile activator, without impairing the contractile machinery itself. 6. The results of the present paper support the view that the time course of potassium contracture is controlled by a membrane mechanism which is activated upon depolarization and later inactivates with time. 7. The effect of caffeine and local anaesthetics can be explained by assuming that the former prolongs the inactivation time course while the latter shortens it.", "contents": "The effect of caffeine and tetracaine on the time course of potassium contractures of single muscle fibres. 1. The time course of potassium contractures can be significantly prolonged by low concentrations of caffeine. 2. This effect of caffeine is not due to impairment of the fibre relaxing system. 3. Under conditions were contractile repriming is delayed (low temperature) an extra amount of activator can be released by caffeine, in addition to that released by potassium. 4. The source of this extra amount of activator is intracellular since its release can be shown in a O calcium EGTA medium. 5. Local anaesthetics, tetracaine, and to a lesser extent procaine, affect the release of contractile activator, without impairing the contractile machinery itself. 6. The results of the present paper support the view that the time course of potassium contracture is controlled by a membrane mechanism which is activated upon depolarization and later inactivates with time. 7. The effect of caffeine and local anaesthetics can be explained by assuming that the former prolongs the inactivation time course while the latter shortens it."}
{"id": "PMID:1082937", "title": "The accuracy of recording patient problems in family practice.", "content": "In 1974 several studies were conducted on the validity of medical data recorded and computerized at family medical centers affiliated with the University of Western Ontario. Fifty-nine encounters were observed. An average of 2.54 somatic, emotional, or social problems were dealt with per encounter. The residents recorded an average of 1.51 problems and the observers 2.45. This difference is highly statistically significant (p less than .001). There was no significant statistical differences among the observers. The many questions this study raises may have a bearing on medical education, medical audit, research, medical computer systems, and perhaps even on quality of care since problem-solving is based on problem identification. Further studies and evaluation are needed.", "contents": "The accuracy of recording patient problems in family practice. In 1974 several studies were conducted on the validity of medical data recorded and computerized at family medical centers affiliated with the University of Western Ontario. Fifty-nine encounters were observed. An average of 2.54 somatic, emotional, or social problems were dealt with per encounter. The residents recorded an average of 1.51 problems and the observers 2.45. This difference is highly statistically significant (p less than .001). There was no significant statistical differences among the observers. The many questions this study raises may have a bearing on medical education, medical audit, research, medical computer systems, and perhaps even on quality of care since problem-solving is based on problem identification. Further studies and evaluation are needed."}
{"id": "PMID:1082939", "title": "Natural cytotoxic reactivity of rat lymphocytes against syngeneic Gross virus-induced lymphoma.", "content": "Lymphoid cells from many normal W/Fu rats reacted in a 51Cr release cytotoxicity assay against (C58NT)D, a syngeneic Gross leukemia virus-induced tumor. The reactivity was maximal in young rats at 5-8 weeks of age and rapidly declined thereafter. Within reactive rats, the cytotoxicity was widely distributed among the various lymphoid organs. Since immunization of W/Fu rats with (C58NT)D was shown to elicit specific cell-mediated cytotoxic reactivity, studies were done to compare the characteristics of the natural reactivity with those of the immune reactivity. The specificity of both types of reactivity was analyzed in detail by an inhibition assay. The natural and the immune reactivities appeared directed against antigens associated with rat endogenous type-C viruses. The major differences between the natural reactivity and immune reactivity were the nature of the effector cells. Whereas immune reactivity was T-cell dependent, normal reactivity was not affected by treatment with antisera against T cells plus complement. Natural effector cells were not adherent and did not have macrophage properties. The active cells also did not have receptors for Ig or complement. The absence of detectable cell-surface markers on the natural effector cells was seen in studies of natural cytotoxic reactivity of mice, and it is proposed that the natural cytotoxicity in both systems is mediated by a unique subpopulation of lymphoid cells, tentatively designated \"N\" cells.", "contents": "Natural cytotoxic reactivity of rat lymphocytes against syngeneic Gross virus-induced lymphoma. Lymphoid cells from many normal W/Fu rats reacted in a 51Cr release cytotoxicity assay against (C58NT)D, a syngeneic Gross leukemia virus-induced tumor. The reactivity was maximal in young rats at 5-8 weeks of age and rapidly declined thereafter. Within reactive rats, the cytotoxicity was widely distributed among the various lymphoid organs. Since immunization of W/Fu rats with (C58NT)D was shown to elicit specific cell-mediated cytotoxic reactivity, studies were done to compare the characteristics of the natural reactivity with those of the immune reactivity. The specificity of both types of reactivity was analyzed in detail by an inhibition assay. The natural and the immune reactivities appeared directed against antigens associated with rat endogenous type-C viruses. The major differences between the natural reactivity and immune reactivity were the nature of the effector cells. Whereas immune reactivity was T-cell dependent, normal reactivity was not affected by treatment with antisera against T cells plus complement. Natural effector cells were not adherent and did not have macrophage properties. The active cells also did not have receptors for Ig or complement. The absence of detectable cell-surface markers on the natural effector cells was seen in studies of natural cytotoxic reactivity of mice, and it is proposed that the natural cytotoxicity in both systems is mediated by a unique subpopulation of lymphoid cells, tentatively designated \"N\" cells."}
{"id": "PMID:1082940", "title": "Bacterial lipopolysaccharides as helper factors for Friend spleen focus-forming virus in mice.", "content": "Lipopolysaccharide (LPS) from several gram-negative bacteria significantly increased the spleen focus-forming efficiency of N-topic Friend virus complex in mice by different  mechanisms. One effect was associated with an increase in the number of availability of potential target cells for spleen focus-forming virus (SFFV) in fully susceptible mouse strains. This enhancing effect was optimal when LPS was injected 5 days before SFFV but was nil when LPS and SFFV were given at the same time. In contrast, in mice genetically resistant to the native helper virus of SFFV, the helper effect of LPS was optical when it was injected with SFFV and oil when given 5 days before or after the virus. LPS did not affect helper virus expression in a standard cell culture (XC) assay, but it did increase helper virus replication in mice. Mice lacking T cells or complete endogenous murine leukemia virus genomes were just as sensitive to the helper effects of LPS on SFFV expression as were control animals. Most of the helper activity of LPS is associated with the lipid A component. The mechanism of the helper effect of lipid A is still unknown, but hypotheses must take into account that this effect did not occur in fully susceptible hosts, but only in hosts carrying resistance alleles at either the FV-1 or the FV-2 locus.", "contents": "Bacterial lipopolysaccharides as helper factors for Friend spleen focus-forming virus in mice. Lipopolysaccharide (LPS) from several gram-negative bacteria significantly increased the spleen focus-forming efficiency of N-topic Friend virus complex in mice by different  mechanisms. One effect was associated with an increase in the number of availability of potential target cells for spleen focus-forming virus (SFFV) in fully susceptible mouse strains. This enhancing effect was optimal when LPS was injected 5 days before SFFV but was nil when LPS and SFFV were given at the same time. In contrast, in mice genetically resistant to the native helper virus of SFFV, the helper effect of LPS was optical when it was injected with SFFV and oil when given 5 days before or after the virus. LPS did not affect helper virus expression in a standard cell culture (XC) assay, but it did increase helper virus replication in mice. Mice lacking T cells or complete endogenous murine leukemia virus genomes were just as sensitive to the helper effects of LPS on SFFV expression as were control animals. Most of the helper activity of LPS is associated with the lipid A component. The mechanism of the helper effect of lipid A is still unknown, but hypotheses must take into account that this effect did not occur in fully susceptible hosts, but only in hosts carrying resistance alleles at either the FV-1 or the FV-2 locus."}
{"id": "PMID:1082941", "title": "The nonselective cytotoxic cell (N cell).", "content": "When effector lymphocytes were reacted with cultured human tumors, the total cytotoxic reaction could be divided into selective and nonselective components. The nonselective part of the reaction was due to a cell type called N cells. Fractionation of effector suspension indicated that N cells were neither T nor B cells. Like B cells, N cells did not form rosettes with sheep erythrocytes; they were retained by columns coated with lg or antiserum to lg and died preferentially when stored at an ambient temperature. However, N cells were differentiated from B cells by their inability to form complement-receptor rosettes and by their survival when incubated at 30 degrees C. The effect of the nonselective cytotoxic cell must be differentiated from selective activity in studies of specificity for cell-mediated cytotoxicity.", "contents": "The nonselective cytotoxic cell (N cell). When effector lymphocytes were reacted with cultured human tumors, the total cytotoxic reaction could be divided into selective and nonselective components. The nonselective part of the reaction was due to a cell type called N cells. Fractionation of effector suspension indicated that N cells were neither T nor B cells. Like B cells, N cells did not form rosettes with sheep erythrocytes; they were retained by columns coated with lg or antiserum to lg and died preferentially when stored at an ambient temperature. However, N cells were differentiated from B cells by their inability to form complement-receptor rosettes and by their survival when incubated at 30 degrees C. The effect of the nonselective cytotoxic cell must be differentiated from selective activity in studies of specificity for cell-mediated cytotoxicity."}
{"id": "PMID:1082942", "title": "Posterior fossa tumors with pathological laughter.", "content": "Of two patients with posterior fossa tumor and pathological laughter, one had extrinsic compression of the brain stem; the other had an intrinsic brain-stem lesion. Surgical removal of the epidermoid from the cerebellopontine angle cured the laughter. The euphoria of multiple sclerosis with paroxysmal laughter and a feeling of joy is to be differentiated from cases in which pathological laughter is a presenting complaint.", "contents": "Posterior fossa tumors with pathological laughter. Of two patients with posterior fossa tumor and pathological laughter, one had extrinsic compression of the brain stem; the other had an intrinsic brain-stem lesion. Surgical removal of the epidermoid from the cerebellopontine angle cured the laughter. The euphoria of multiple sclerosis with paroxysmal laughter and a feeling of joy is to be differentiated from cases in which pathological laughter is a presenting complaint."}
{"id": "PMID:1082943", "title": "Barium impaction as a complication of gastrointestinal scleroderma.", "content": "Two patients with scleroderma of the bowel experienced life-threatening barium impaction after upper intestinal x-ray studies. Although the frequency of this complication is unknown, the difficulty of managing it when it occurs makes prevention imperative. X-ray studies should be performed only after careful consideration of the risks and benefits. When x-ray studies are performed, the patient should be vigorously purged soon thereafter, and a follow-up roentgenogram should be obtained to confirm adequate removal of barium.", "contents": "Barium impaction as a complication of gastrointestinal scleroderma. Two patients with scleroderma of the bowel experienced life-threatening barium impaction after upper intestinal x-ray studies. Although the frequency of this complication is unknown, the difficulty of managing it when it occurs makes prevention imperative. X-ray studies should be performed only after careful consideration of the risks and benefits. When x-ray studies are performed, the patient should be vigorously purged soon thereafter, and a follow-up roentgenogram should be obtained to confirm adequate removal of barium."}
{"id": "PMID:1082945", "title": "[Effect of temperature on excitation-contraction coupling of the bullfrog atrium (author's transl)].", "content": "Numerous studies have been reported on the effects of temperature on the electrical and mechanical properties of myocardium, and the augmentation of tension in low temperature has been generally considered to be simply due to a marked prolongation of the membrane action potential. In recent years, however, several kinds of the membrane currents such as INa1, INa2, ICa, IK1, IK2, IX1, IX2 and IC1 were discovered on the myocardium and the complicated processes of membrane excitation-contraction coupling (E-C coupling) were greatly analized in relation with the roles of these membrane currents and sarcoplasmic reticulum. Accordingly, the mechanisms of the inotropic effect of low temperature must also be reexamined on the basis of these lately developed knowledges. The present study, therefore, was undertaken to elucidate fundamental mechanisms of the action of temperature upon the membrane currents and E-C coupling processes, by examining the frog heart muscle with voltage clamp technique. The preparation used in the present experiments was the atrial muscle bundle isolated from the bullfrog (Rana cathesbiana). The thin muscle bundle was about 7 mm in length and 0.6-0.8 mm in diameter. The membrane potential, current and contractile tension were measured by means of the double glycerol-gap technique in voltage clamped conditions. The muscle chamber consisted of five compartments (1, 2, 3, 4 and 5). The large terminal compartments (1 and 5) were flowed with 200 mM KCl solution, the intermediate compartments (2 and 4) with isotonic glycerol solution, and the central compartment with Ringer or test solutions. The width of glycerol gap was 1 mm, and that of the central compartment ranged from 0.3 to 0.6 mm. The membrane potential was obtained as the potential difference between the central and terminal compartments (3 and 5), and the current, from the central compartment (3) which was kept at nearly earth potential by the aid of an operational amplifier (Philbrick, P65AU). The muscle tension was measured by the aid of a strain gauge (Nihon Kohden, SB-1T), the sensitive arm of which was connected to the end of muscle bundle in the terminal compartment (5). These potential, current and tension were recorded simultaneously utilizing by a triad-beam oscilloscope (Nihon Kohden, VC-7) and a inkwriting oscilloscope (Nihon Kohden, WI-180).", "contents": "[Effect of temperature on excitation-contraction coupling of the bullfrog atrium (author's transl)]. Numerous studies have been reported on the effects of temperature on the electrical and mechanical properties of myocardium, and the augmentation of tension in low temperature has been generally considered to be simply due to a marked prolongation of the membrane action potential. In recent years, however, several kinds of the membrane currents such as INa1, INa2, ICa, IK1, IK2, IX1, IX2 and IC1 were discovered on the myocardium and the complicated processes of membrane excitation-contraction coupling (E-C coupling) were greatly analized in relation with the roles of these membrane currents and sarcoplasmic reticulum. Accordingly, the mechanisms of the inotropic effect of low temperature must also be reexamined on the basis of these lately developed knowledges. The present study, therefore, was undertaken to elucidate fundamental mechanisms of the action of temperature upon the membrane currents and E-C coupling processes, by examining the frog heart muscle with voltage clamp technique. The preparation used in the present experiments was the atrial muscle bundle isolated from the bullfrog (Rana cathesbiana). The thin muscle bundle was about 7 mm in length and 0.6-0.8 mm in diameter. The membrane potential, current and contractile tension were measured by means of the double glycerol-gap technique in voltage clamped conditions. The muscle chamber consisted of five compartments (1, 2, 3, 4 and 5). The large terminal compartments (1 and 5) were flowed with 200 mM KCl solution, the intermediate compartments (2 and 4) with isotonic glycerol solution, and the central compartment with Ringer or test solutions. The width of glycerol gap was 1 mm, and that of the central compartment ranged from 0.3 to 0.6 mm. The membrane potential was obtained as the potential difference between the central and terminal compartments (3 and 5), and the current, from the central compartment (3) which was kept at nearly earth potential by the aid of an operational amplifier (Philbrick, P65AU). The muscle tension was measured by the aid of a strain gauge (Nihon Kohden, SB-1T), the sensitive arm of which was connected to the end of muscle bundle in the terminal compartment (5). These potential, current and tension were recorded simultaneously utilizing by a triad-beam oscilloscope (Nihon Kohden, VC-7) and a inkwriting oscilloscope (Nihon Kohden, WI-180)."}
{"id": "PMID:1082962", "title": "[Autolymphocytotoxins (CoCoCy) in different forms of hepatitis and cirrhosis (author's transl)].", "content": "Sera of altogether 282 patients with different forms of hepatitis and cirrhosis were screened for cold reacting complement dependant auto-lympho-cytotoxins (CoCoCy). These antibodies are 19S-IgM-immunoglobins and have no HLA-antigen-specificity. CoCoCy occurred in 48% of the patients with chronic aggressive hepatitis (CAH), in 14% of the patients with chronic persistent hepatitis (CPH) and in intermediate rates in the sera of patients with acute hepatitis. No correlations was found between CoCoCy and hepatitis B-antigen (HB-Ag). CoCoCy could be demonstrated also in 20% of the sera of a HB-Ag-positive and in 6% of a HB-Ag-negative control group. The serum concentration of CoCoCy is low. CoCoCy seems to be of T-cell-specificity and to reflect the overall-immunoreactivity without relation to the specificity of the antigenic stimulus. Thus demonstration of CoCoCy may be of pathogeneic and pathodynamic rather than of diagnostic interest.", "contents": "[Autolymphocytotoxins (CoCoCy) in different forms of hepatitis and cirrhosis (author's transl)]. Sera of altogether 282 patients with different forms of hepatitis and cirrhosis were screened for cold reacting complement dependant auto-lympho-cytotoxins (CoCoCy). These antibodies are 19S-IgM-immunoglobins and have no HLA-antigen-specificity. CoCoCy occurred in 48% of the patients with chronic aggressive hepatitis (CAH), in 14% of the patients with chronic persistent hepatitis (CPH) and in intermediate rates in the sera of patients with acute hepatitis. No correlations was found between CoCoCy and hepatitis B-antigen (HB-Ag). CoCoCy could be demonstrated also in 20% of the sera of a HB-Ag-positive and in 6% of a HB-Ag-negative control group. The serum concentration of CoCoCy is low. CoCoCy seems to be of T-cell-specificity and to reflect the overall-immunoreactivity without relation to the specificity of the antigenic stimulus. Thus demonstration of CoCoCy may be of pathogeneic and pathodynamic rather than of diagnostic interest."}
{"id": "PMID:1082965", "title": "[Allogenic and xenogenic aortic segmental transplants in inbred rats angiographically demonstrable vascular changes (author's transl)].", "content": "In order to make a systematic study of the significance of immunological reactions in vascular grafts, segments of abdominal aorta (4-5 mm long) were grafted into inbred rats. Four experimental combinations were used: I. syngeneic (CDF leads to CDF), II. weakly allogeneic (LEW leads to CDF), III. strongly allogeneic (BD 5 leads to CDF), IV. xenogeneic (guinea pig leads to CDF). After 100 days the gaft was macroscopically evaluated, angiographed and measured. Whereas no alterations of the vascular wall were found in group I, the degree of aneurysm formation increased significantly corresponding to the degree of histoincompatibility in the other groups: group II showed little aneurysm formation (medicum size 1.27 fold), group III more pronounced (1.71 fold) and group IV very pronounced (3.9 fold) with partial thrombosis in 3/5 of the cases. These findings are interpreted, as functional and morphological consequences of the immunogenicity of the aorta tissue. The conclusion was reached that the degree of histoincompatibility should be more carefully considered, also in vascular grafting.", "contents": "[Allogenic and xenogenic aortic segmental transplants in inbred rats angiographically demonstrable vascular changes (author's transl)]. In order to make a systematic study of the significance of immunological reactions in vascular grafts, segments of abdominal aorta (4-5 mm long) were grafted into inbred rats. Four experimental combinations were used: I. syngeneic (CDF leads to CDF), II. weakly allogeneic (LEW leads to CDF), III. strongly allogeneic (BD 5 leads to CDF), IV. xenogeneic (guinea pig leads to CDF). After 100 days the gaft was macroscopically evaluated, angiographed and measured. Whereas no alterations of the vascular wall were found in group I, the degree of aneurysm formation increased significantly corresponding to the degree of histoincompatibility in the other groups: group II showed little aneurysm formation (medicum size 1.27 fold), group III more pronounced (1.71 fold) and group IV very pronounced (3.9 fold) with partial thrombosis in 3/5 of the cases. These findings are interpreted, as functional and morphological consequences of the immunogenicity of the aorta tissue. The conclusion was reached that the degree of histoincompatibility should be more carefully considered, also in vascular grafting."}
{"id": "PMID:1082966", "title": "Temporal bone histopathology in experimental hypovitaminosis A.", "content": "Twenty-five years ago hearing loss was observed in some subjects during a comprehensive study of the effects of hypovitaminosis A on human volunteers. Experimental studies documenting histopathological changes in the temporal bone due to hypovitaminosis A are conflicting. Even the recent textbooks of otolaryngology and physiology make no mention of a role of vitamin A in the ear. To explore the role of vitamin A in the ear adult and weanling rats maintained on a diet totally lacking vitamin A were sacrificed at intervals. Their temporal bones were examined with the light microscope. After six weeks on a vitamin A free diet weanling rats showed hypertrophy of the periostial portions of the otic capsule. At 16 weeks a narrowing of the internal auditory canal due to bony exostoses was present. The neuroepithelia of the cochlea and the vestibular apparatus were histologically normal even in the longest surfiving animals. Adult rats maintained on a vitamin A free diet showed minimal thickening of the bone adjacent to the internal auditory meatus. The cochlea and the vestibular appartus in these animals remained normal throughout the 28-week experiment. Although we have demonstrated marked abnormalities of the otic capsule in hypovitaminosis A, our results do not support those of some earlier investigators who reported that atrophy of the cochlear and vestibular neuroepithelium occurred in the absence of dietary vitamin A.", "contents": "Temporal bone histopathology in experimental hypovitaminosis A. Twenty-five years ago hearing loss was observed in some subjects during a comprehensive study of the effects of hypovitaminosis A on human volunteers. Experimental studies documenting histopathological changes in the temporal bone due to hypovitaminosis A are conflicting. Even the recent textbooks of otolaryngology and physiology make no mention of a role of vitamin A in the ear. To explore the role of vitamin A in the ear adult and weanling rats maintained on a diet totally lacking vitamin A were sacrificed at intervals. Their temporal bones were examined with the light microscope. After six weeks on a vitamin A free diet weanling rats showed hypertrophy of the periostial portions of the otic capsule. At 16 weeks a narrowing of the internal auditory canal due to bony exostoses was present. The neuroepithelia of the cochlea and the vestibular apparatus were histologically normal even in the longest surfiving animals. Adult rats maintained on a vitamin A free diet showed minimal thickening of the bone adjacent to the internal auditory meatus. The cochlea and the vestibular appartus in these animals remained normal throughout the 28-week experiment. Although we have demonstrated marked abnormalities of the otic capsule in hypovitaminosis A, our results do not support those of some earlier investigators who reported that atrophy of the cochlear and vestibular neuroepithelium occurred in the absence of dietary vitamin A."}
{"id": "PMID:1082967", "title": "[Hemobilia (author's transl)].", "content": "Hemobilia is a syndrome caused by hemorrhage into the biliary ducts. It is characterized by colicky pains, jaundice, and intestinal bleeding; a correct diagnosis of this syndrome is made rather rarely. The most common cause is trauma; inflammatory or tumorous disease of the liver and bile duct system, bile stones and aneurysms of the hepatic artery are rarer causes. The diagnosis can be established and the arterio-biliary fistula localized by selective arteriography. A case is described, which shows, that, in addition, duodenoscopy with retrograde cholangiography is of diagnostic value. Other helpful diagnostic procedures are: splenoportography, liver scanning, and intraoperative cholangiography. Therapy must be surgical exclusively; palliative operations lead to high rates of relapses and letality. Best results are achieved by ligature of the hepatic artery in cases of central, or by partial resection of the liver in cases of peripheral localization of the fistula.", "contents": "[Hemobilia (author's transl)]. Hemobilia is a syndrome caused by hemorrhage into the biliary ducts. It is characterized by colicky pains, jaundice, and intestinal bleeding; a correct diagnosis of this syndrome is made rather rarely. The most common cause is trauma; inflammatory or tumorous disease of the liver and bile duct system, bile stones and aneurysms of the hepatic artery are rarer causes. The diagnosis can be established and the arterio-biliary fistula localized by selective arteriography. A case is described, which shows, that, in addition, duodenoscopy with retrograde cholangiography is of diagnostic value. Other helpful diagnostic procedures are: splenoportography, liver scanning, and intraoperative cholangiography. Therapy must be surgical exclusively; palliative operations lead to high rates of relapses and letality. Best results are achieved by ligature of the hepatic artery in cases of central, or by partial resection of the liver in cases of peripheral localization of the fistula."}
{"id": "PMID:1082971", "title": "Chronic cutaneous lupus erythematosus (DLE)--a clinical and laboratory investigation of 80 patients.", "content": "Clinical and laboratory data are presented from a study of a group of 80 patients with chronic cutaneous discoid lupus erythematosus. These data support the contention that if one selects patients with chronic scarring DLE who have no evidence by history or physical examination of extracutaneous involvement, then only a small percentage of patients will have detectable immunologic derangements. It is apparent that the clinical expression of lupus erythematosus depends, in part, upon the nature of the host's immune response.", "contents": "Chronic cutaneous lupus erythematosus (DLE)--a clinical and laboratory investigation of 80 patients. Clinical and laboratory data are presented from a study of a group of 80 patients with chronic cutaneous discoid lupus erythematosus. These data support the contention that if one selects patients with chronic scarring DLE who have no evidence by history or physical examination of extracutaneous involvement, then only a small percentage of patients will have detectable immunologic derangements. It is apparent that the clinical expression of lupus erythematosus depends, in part, upon the nature of the host's immune response."}
{"id": "PMID:1082994", "title": "[Angiographic diagnosis of non-neoplastic aqueductal stenosis in children (author's transl)].", "content": "Angiograpic diagnosis of non-neoplastic aqueductal stenosis in 4 children were described. The materials were selected from 160 vertebral angiograms of children below 15 years, performed at the Kyushu University Hospital from July 1967 to March 1974. There were 4 cases of angiographically demonstrated non-neoplastic aqueductal stenosis. These cases were confirmed by pneumoence-phalogram and/or pneumoventriculograms, which were performed before and after the Tolkildens procedure. Ages of the patients varied from 6 to 15 years with average of 11.8 years. All the angiographic findings of these 4 patients with non-neoplastic aqueductal stenosis were explained on the basis of 5 major morphological changes such as 1) marked symmetrical obstructive hydrocephalus, 2) dilatation of the third ventricle and aqueduct above the obstruction with resultant downward displacement and compression up the upper brain stem and posterior displacement of the precentral cerebellar fissure, 3) maintenance of normal shape and position of the lower half of the posterior fossa structure, 4) absence of midline shaft of the median and paramedian vessels, 5) morphologic changes of the subarachonoid cisterns. These angiographic findings coincided with the angiographic features as described by Huang et al. (1968), under the experience with 10 cases of non-neoplastic aqueductal stenosis of adults, averaging of 43 years. Special emphasis was placed on the diagnostic value of the small veins and arteries in the posterior fossa vascular system. We also emphasized that the vertebral angiography should be performed as the first investigative procedure using the contrast media in the posterior fossa lesions.", "contents": "[Angiographic diagnosis of non-neoplastic aqueductal stenosis in children (author's transl)]. Angiograpic diagnosis of non-neoplastic aqueductal stenosis in 4 children were described. The materials were selected from 160 vertebral angiograms of children below 15 years, performed at the Kyushu University Hospital from July 1967 to March 1974. There were 4 cases of angiographically demonstrated non-neoplastic aqueductal stenosis. These cases were confirmed by pneumoence-phalogram and/or pneumoventriculograms, which were performed before and after the Tolkildens procedure. Ages of the patients varied from 6 to 15 years with average of 11.8 years. All the angiographic findings of these 4 patients with non-neoplastic aqueductal stenosis were explained on the basis of 5 major morphological changes such as 1) marked symmetrical obstructive hydrocephalus, 2) dilatation of the third ventricle and aqueduct above the obstruction with resultant downward displacement and compression up the upper brain stem and posterior displacement of the precentral cerebellar fissure, 3) maintenance of normal shape and position of the lower half of the posterior fossa structure, 4) absence of midline shaft of the median and paramedian vessels, 5) morphologic changes of the subarachonoid cisterns. These angiographic findings coincided with the angiographic features as described by Huang et al. (1968), under the experience with 10 cases of non-neoplastic aqueductal stenosis of adults, averaging of 43 years. Special emphasis was placed on the diagnostic value of the small veins and arteries in the posterior fossa vascular system. We also emphasized that the vertebral angiography should be performed as the first investigative procedure using the contrast media in the posterior fossa lesions."}
{"id": "PMID:1082995", "title": "Measurements of the normal ventricular system with computer tomography of the brain. A preliminary study on 44 adults.", "content": "The brains of 44 normal adults were examined by computer tomography (CT) with the fine matrix EMI scanner. Measurements of various parts of the ventricular system showed a sexual difference and an increase with age. Absolute measurements are preferred to indices or ratios. Comparison with the same parameters in pneumoncephalography and ventricular casts showed good correlation. The need for larger investigations of the normal population  is stressed.", "contents": "Measurements of the normal ventricular system with computer tomography of the brain. A preliminary study on 44 adults. The brains of 44 normal adults were examined by computer tomography (CT) with the fine matrix EMI scanner. Measurements of various parts of the ventricular system showed a sexual difference and an increase with age. Absolute measurements are preferred to indices or ratios. Comparison with the same parameters in pneumoncephalography and ventricular casts showed good correlation. The need for larger investigations of the normal population  is stressed."}
{"id": "PMID:1082997", "title": "[Ankylosing hemorrhagic arthropathy in a patient with Von Willebrand's disease (angiohemophilia A)].", "content": "A case of von Willebrand's disease with unusual clinical manifestations is described. The disease was characterized by the presence of insistently recurring haemarthroses, starting from infancy, involving a number of joints, ending in ankylosis. Bleeding time was lengthened and the patient presented antihaemophilic globulin deficiency and decreased platelet adhesiveness.", "contents": "[Ankylosing hemorrhagic arthropathy in a patient with Von Willebrand's disease (angiohemophilia A)]. A case of von Willebrand's disease with unusual clinical manifestations is described. The disease was characterized by the presence of insistently recurring haemarthroses, starting from infancy, involving a number of joints, ending in ankylosis. Bleeding time was lengthened and the patient presented antihaemophilic globulin deficiency and decreased platelet adhesiveness."}
{"id": "PMID:1083007", "title": "Relapse of Hemophilus influenzae type b meningitis after combined antibiotic therapy: report of a case.", "content": "Antibiotic therapy of bacterial meningitis is being reevaluated due to reports of ampicillin-resistant strains of Hemophilus influenzae type b. The infant reported had a relapse of H. influenzae type b meningitis after an excellent clinical and bacteriologic response to an initial course of combined antibiotic therapy including chloramphenicol. This relapse is postulated to be due to localized cerebral vasculitis which was not treated for a sufficient period of time during the initial course of therapy. The patient responded well to a second course of penicillin and chloramphenicol. Since the use of pencillin and chloramphenicol will be increasing, the clinician should be aware that bacteriologic relapse of H. influenzae type b meningitis may occur with chloramphenicol therapy.", "contents": "Relapse of Hemophilus influenzae type b meningitis after combined antibiotic therapy: report of a case. Antibiotic therapy of bacterial meningitis is being reevaluated due to reports of ampicillin-resistant strains of Hemophilus influenzae type b. The infant reported had a relapse of H. influenzae type b meningitis after an excellent clinical and bacteriologic response to an initial course of combined antibiotic therapy including chloramphenicol. This relapse is postulated to be due to localized cerebral vasculitis which was not treated for a sufficient period of time during the initial course of therapy. The patient responded well to a second course of penicillin and chloramphenicol. Since the use of pencillin and chloramphenicol will be increasing, the clinician should be aware that bacteriologic relapse of H. influenzae type b meningitis may occur with chloramphenicol therapy."}
{"id": "PMID:1083014", "title": "[Digestive tract hemorrhages of cirrhotic patients. Relation between hepatic insufficiency and the hemorrhagic lesion].", "content": "The cause of upper gastrointestinal bleeding was studied in 85 cirrhotic patients by emergency endoscopy. In every patient, one or several lesions were observed and the site of bleeding was ascertained in 59. The two main causes were oesophago-gastric varices (46 p.cent) and acute mucosal lesions(42 p.cent). The source of bleeding appeared to be related to the degree of liver function impairment. In patients with no or moderate liver function impariment, bleeding usually originated from varices or from drug-associated mucosal erosions. Patients with severe impairment of liver function most often bled from spontaneous acute oeso-gastro-duodenal ulcerations. These ulcerations resembled what has been described in \"stress\" hemorrhage. Because of the relationship between liver function and the cause of hemorrhage, mortality was lower in variceal bleeders (29 p.cent) than in patients with spontaneous ulcerations (83 p.cent). In patients with severely impaired liver function, portacaval shunt was rarely indicated since hemorrhage was generally due to acute mucosal ulcerations.", "contents": "[Digestive tract hemorrhages of cirrhotic patients. Relation between hepatic insufficiency and the hemorrhagic lesion]. The cause of upper gastrointestinal bleeding was studied in 85 cirrhotic patients by emergency endoscopy. In every patient, one or several lesions were observed and the site of bleeding was ascertained in 59. The two main causes were oesophago-gastric varices (46 p.cent) and acute mucosal lesions(42 p.cent). The source of bleeding appeared to be related to the degree of liver function impairment. In patients with no or moderate liver function impariment, bleeding usually originated from varices or from drug-associated mucosal erosions. Patients with severe impairment of liver function most often bled from spontaneous acute oeso-gastro-duodenal ulcerations. These ulcerations resembled what has been described in \"stress\" hemorrhage. Because of the relationship between liver function and the cause of hemorrhage, mortality was lower in variceal bleeders (29 p.cent) than in patients with spontaneous ulcerations (83 p.cent). In patients with severely impaired liver function, portacaval shunt was rarely indicated since hemorrhage was generally due to acute mucosal ulcerations."}
{"id": "PMID:1083016", "title": "Immunotherapy: concepts and nursing implications.", "content": "Concepts of immunlogy and the human immune system are essential for an understanding of the rationale of immunotherapy. Immunotherapy is presently being investigated as adjunct therapy for patients with cancer. It is therefore essential for the professional nurse to become cognizant of the various modalities and possible complications of immunotherapy in order that the nursing implications may be incorporated into clinical practice.", "contents": "Immunotherapy: concepts and nursing implications. Concepts of immunlogy and the human immune system are essential for an understanding of the rationale of immunotherapy. Immunotherapy is presently being investigated as adjunct therapy for patients with cancer. It is therefore essential for the professional nurse to become cognizant of the various modalities and possible complications of immunotherapy in order that the nursing implications may be incorporated into clinical practice."}
{"id": "PMID:1083027", "title": "Effect of concanavalin A on expression of cell surface sialyltransferase activity of mouse thymocytes.", "content": "Incubation of mouse thymocytes with mitogenic concentrations of concanavalin A causes a 2-fold increase in cell-surface-associated (but not total cell) sialyltransferase activity (ectosialyltransferase, CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) as judged by incorporation of [14C]sialic acid into endogenous cell acceptors and into added desialylated fetuin acceptor. The concanavalin-A-induced enhancement of enzymic activity is essentially complete within 1 hr after addition of mitogen and remains at elevated levels for 12 hr, declining rapidly thereafter. Intact cells labeled previously with [14C]sialic acid and then incubated briefly with hydrolytic enzymes, including neuraminidase and insoluble trypsin, released 43-66% of total cell-associated radioactivity without appreciably changing cell viability. Alterations in sialyltransferase activity due to concanavalin A treatment could not be explained by a mitogen-mediated (a) uptake of radioactive precursors, (b) cell death, (c) increased product catabolism, or (d) activation of sialyltransferase by mitogen binding to the enzyme. Furthermore, the process does not require active protein synthesis. The results are consistent with a rapid concanavalin-A-induced exposure of potential enzymic activity that was previously inaccessible to substrate.", "contents": "Effect of concanavalin A on expression of cell surface sialyltransferase activity of mouse thymocytes. Incubation of mouse thymocytes with mitogenic concentrations of concanavalin A causes a 2-fold increase in cell-surface-associated (but not total cell) sialyltransferase activity (ectosialyltransferase, CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) as judged by incorporation of [14C]sialic acid into endogenous cell acceptors and into added desialylated fetuin acceptor. The concanavalin-A-induced enhancement of enzymic activity is essentially complete within 1 hr after addition of mitogen and remains at elevated levels for 12 hr, declining rapidly thereafter. Intact cells labeled previously with [14C]sialic acid and then incubated briefly with hydrolytic enzymes, including neuraminidase and insoluble trypsin, released 43-66% of total cell-associated radioactivity without appreciably changing cell viability. Alterations in sialyltransferase activity due to concanavalin A treatment could not be explained by a mitogen-mediated (a) uptake of radioactive precursors, (b) cell death, (c) increased product catabolism, or (d) activation of sialyltransferase by mitogen binding to the enzyme. Furthermore, the process does not require active protein synthesis. The results are consistent with a rapid concanavalin-A-induced exposure of potential enzymic activity that was previously inaccessible to substrate."}
{"id": "PMID:1083028", "title": "Novel mechanism for translational control in regulation of ferritin synthesis by iron.", "content": "Poly(A)-containing RNA was isolated from the polyribosomal and post-ribosomal fractions of the livers of normal and iron-treated rats. These RNA fractions were then translated in a wheat germ system to provide a measure of the amount of ferritin mRNA present in each fraction. Following iron administration, there was a 2-fold increase in the amount of ferritin mRNA in the polyribosomal fraction. This increase was not inhibited by prior treatment of the rats with actinomycin D or cordycepin, suggesting a cytoplasmic control mechanism. In normal rats, the post-ribosomal fraction contained an amount of ferritin mRNA equal to that in the polyribosomes. When iron was administered, this untranslated ferritin mRNA became reduced to negligible quantities, thus accounting for the doubling of the ferritin mRNA content of the polyribosomal fraction. A scheme is proposed in which translation of the ferritin mRNA in the post-ribosomal fraction is prevented by adhering ferritin subunits. Iron administration removes this inhibition of the translation of ferritin mRNA by promoting aggregation of these subunits into ferritin.", "contents": "Novel mechanism for translational control in regulation of ferritin synthesis by iron. Poly(A)-containing RNA was isolated from the polyribosomal and post-ribosomal fractions of the livers of normal and iron-treated rats. These RNA fractions were then translated in a wheat germ system to provide a measure of the amount of ferritin mRNA present in each fraction. Following iron administration, there was a 2-fold increase in the amount of ferritin mRNA in the polyribosomal fraction. This increase was not inhibited by prior treatment of the rats with actinomycin D or cordycepin, suggesting a cytoplasmic control mechanism. In normal rats, the post-ribosomal fraction contained an amount of ferritin mRNA equal to that in the polyribosomes. When iron was administered, this untranslated ferritin mRNA became reduced to negligible quantities, thus accounting for the doubling of the ferritin mRNA content of the polyribosomal fraction. A scheme is proposed in which translation of the ferritin mRNA in the post-ribosomal fraction is prevented by adhering ferritin subunits. Iron administration removes this inhibition of the translation of ferritin mRNA by promoting aggregation of these subunits into ferritin."}
{"id": "PMID:1083029", "title": "Immediate short-time effects of ethacrynic acid on frog gastric mucosa in chloride-free solutions.", "content": "The presence of ethacrynic acid in the nutrient sulphate solution produces, generally in less than 3 min, a decrease in resistance accompanied by an increase in transmucosal PD. These results strongly suggest that the dominant effect of ethacrynic acid is not on the Na+ pathway of the nutrient membrane, but without further knowledge of the resistance of other ionic pathways, it cannot be inferred whether the effect predominates on the K+ pathway as in Cl- media.", "contents": "Immediate short-time effects of ethacrynic acid on frog gastric mucosa in chloride-free solutions. The presence of ethacrynic acid in the nutrient sulphate solution produces, generally in less than 3 min, a decrease in resistance accompanied by an increase in transmucosal PD. These results strongly suggest that the dominant effect of ethacrynic acid is not on the Na+ pathway of the nutrient membrane, but without further knowledge of the resistance of other ionic pathways, it cannot be inferred whether the effect predominates on the K+ pathway as in Cl- media."}
{"id": "PMID:1083030", "title": "Evidence for clustering of H-2K, H-2D, and the Fc receptor on the membranes of B cells.", "content": "Alloantisera to H-2K, H-2D, and Ia antigens markedly inhibited the binding of EA but not FITC-IgG by the B cell Fc receptor. EA rosette formation approached normal levels when masked H-2 but not Ia specificities were allowed to cap on the membranes of B cells. beta2-mu coated SRBC were bound by the Fc receptor, and high concentrations of soluble beta2-mu were found to moderately inhibit EA rosette formation while lower concentrations enhanced binding. The data support the concept of Fc/Ia identity, and they suggest that H-2K, H-2D, and the Fc receptor may be closely grouped on the membranes of B cells. Further, these observations suggest that the beta2-microglobulin associated with H-2 could serve to link T cells with the Fc receptor of B cells during the inductive phase of antibody synthesis.", "contents": "Evidence for clustering of H-2K, H-2D, and the Fc receptor on the membranes of B cells. Alloantisera to H-2K, H-2D, and Ia antigens markedly inhibited the binding of EA but not FITC-IgG by the B cell Fc receptor. EA rosette formation approached normal levels when masked H-2 but not Ia specificities were allowed to cap on the membranes of B cells. beta2-mu coated SRBC were bound by the Fc receptor, and high concentrations of soluble beta2-mu were found to moderately inhibit EA rosette formation while lower concentrations enhanced binding. The data support the concept of Fc/Ia identity, and they suggest that H-2K, H-2D, and the Fc receptor may be closely grouped on the membranes of B cells. Further, these observations suggest that the beta2-microglobulin associated with H-2 could serve to link T cells with the Fc receptor of B cells during the inductive phase of antibody synthesis."}
{"id": "PMID:1083031", "title": "Use of low intensity direct current in management of ischemic skin ulcers.", "content": "A clinical investigation of the use of low intensity direct current (LIDC) in treating ischemic skin ulcers was conducted at the University of Missouri. The purpose of the study was to evaluate the effect of LIDC on altering the healing rate of ischemic skin ulcers. One hundred ulcers were treated with LIDC. Six of the patients had bilateral symmetrical ulcers which provided the control group. The six control ulcers were managed exactly the same as their counterpart  except that LIDC was omitted. The mean healing ratio of the control ulcers was 14.7 percent per week compared to 30 percent per week of the treated counterpart. The mean healing rate of the 100 ischemic skin ulcers treated with LIDC was 28.4 percent per week. The data indicated that ischemic skin ulcers treated with the current responded with a healing rate approximately twice as fast as those ulcers that did not receive LIDC treatment. The authors concluded that the use of LIDC added an effective modality to the armamentarium of the physical therapist for the treatment of ischemic skin ulcers.", "contents": "Use of low intensity direct current in management of ischemic skin ulcers. A clinical investigation of the use of low intensity direct current (LIDC) in treating ischemic skin ulcers was conducted at the University of Missouri. The purpose of the study was to evaluate the effect of LIDC on altering the healing rate of ischemic skin ulcers. One hundred ulcers were treated with LIDC. Six of the patients had bilateral symmetrical ulcers which provided the control group. The six control ulcers were managed exactly the same as their counterpart  except that LIDC was omitted. The mean healing ratio of the control ulcers was 14.7 percent per week compared to 30 percent per week of the treated counterpart. The mean healing rate of the 100 ischemic skin ulcers treated with LIDC was 28.4 percent per week. The data indicated that ischemic skin ulcers treated with the current responded with a healing rate approximately twice as fast as those ulcers that did not receive LIDC treatment. The authors concluded that the use of LIDC added an effective modality to the armamentarium of the physical therapist for the treatment of ischemic skin ulcers."}
{"id": "PMID:1083035", "title": "Effect of concomitantly administered drugs on the control of long term anticoagulant therapy.", "content": "The stability of control of long term anticoagulant therapy was studied in a group of 277 patients with respect to drug interactions. Increased instability (changes of dose/month of therapy) was related to the number of concomitant drugs taken irrespective of, and in addition to, known specific interactions. Fluctuating cardiac status in patients requiring digoxin and diuretic therapy was a major factor in stabilizing anticoagulant control.", "contents": "Effect of concomitantly administered drugs on the control of long term anticoagulant therapy. The stability of control of long term anticoagulant therapy was studied in a group of 277 patients with respect to drug interactions. Increased instability (changes of dose/month of therapy) was related to the number of concomitant drugs taken irrespective of, and in addition to, known specific interactions. Fluctuating cardiac status in patients requiring digoxin and diuretic therapy was a major factor in stabilizing anticoagulant control."}
{"id": "PMID:1083036", "title": "[Radiological evaluation of the course of pneumocystosis (author's transl)].", "content": "Serial chest X rays were performed on 124 infants in an orphanage with endemic Pneumocystic carinii infection. The infection was serologically and autoptically proven in 98 cases. The interstitial infiltrate in the lungs is not specific for Pneumocystis carinii infection, but is the result of the host reaction, which persists after the antigen has been destroyed. The interstitial infiltrate is therefore neither time-related nor diagnostic and cannot be used as a guideline for therapy. The treatment of the patient is completed, while the unspecific infiltrations persist.", "contents": "[Radiological evaluation of the course of pneumocystosis (author's transl)]. Serial chest X rays were performed on 124 infants in an orphanage with endemic Pneumocystic carinii infection. The infection was serologically and autoptically proven in 98 cases. The interstitial infiltrate in the lungs is not specific for Pneumocystis carinii infection, but is the result of the host reaction, which persists after the antigen has been destroyed. The interstitial infiltrate is therefore neither time-related nor diagnostic and cannot be used as a guideline for therapy. The treatment of the patient is completed, while the unspecific infiltrations persist."}
{"id": "PMID:1083032", "title": "[Rheumatological calcinoses].", "content": "The different types of calcinosis observed in rheumatic patients are described : those most frequently found are periatricular and tendinous ; others that are less frequent are subcutaneous and result from tissue attack (filariasis, repeated microtraumatisms) ; diffuse dystrophic calcinoses of the Thibierge and Weissenbach syndrome ; and lasty, in contrast to the preceding examples, diffuse, dystrophic, and metabolic articular chondrocalcinosis. The etiopathogenesis of these calcinoses remains obscure, but the phenomenon of calciphylaxis seems to be involved.", "contents": "[Rheumatological calcinoses]. The different types of calcinosis observed in rheumatic patients are described : those most frequently found are periatricular and tendinous ; others that are less frequent are subcutaneous and result from tissue attack (filariasis, repeated microtraumatisms) ; diffuse dystrophic calcinoses of the Thibierge and Weissenbach syndrome ; and lasty, in contrast to the preceding examples, diffuse, dystrophic, and metabolic articular chondrocalcinosis. The etiopathogenesis of these calcinoses remains obscure, but the phenomenon of calciphylaxis seems to be involved."}
{"id": "PMID:1083037", "title": "Massive extra-enteric gastrointestinal bleeding: angiographic diagnosis.", "content": "Two patients with massive gastrointestinal bleeding are reported. One bled from an aneurysm of a branch of the left hepatic artery, the blood reaching the bowel through communication with the biliary tree. The second had an aneurysm of a branch of the splenic artery which communicated with the pancreatic duct. This type of bleeding is intermittent and, consequently, actual extravasation of contrast media is not always seen. Therefore, if one sees an aneurysm of a visceral artery, even if it does not directly supply the enteric tract, one should consider the possibility that it is the origin of the hemorrhage. Pathogenesis, diagnostic modalities, and therapeutic implications are discussed.", "contents": "Massive extra-enteric gastrointestinal bleeding: angiographic diagnosis. Two patients with massive gastrointestinal bleeding are reported. One bled from an aneurysm of a branch of the left hepatic artery, the blood reaching the bowel through communication with the biliary tree. The second had an aneurysm of a branch of the splenic artery which communicated with the pancreatic duct. This type of bleeding is intermittent and, consequently, actual extravasation of contrast media is not always seen. Therefore, if one sees an aneurysm of a visceral artery, even if it does not directly supply the enteric tract, one should consider the possibility that it is the origin of the hemorrhage. Pathogenesis, diagnostic modalities, and therapeutic implications are discussed."}
{"id": "PMID:1083056", "title": "Environmental Radioactivity in Denmark in 1974.", "content": "The present report deals with the measurement of fall-out radioactivity in Denmark in 1974. Strontium-90 was determined in samples from all over the country of precipitation, soil, ground water, sea water, grass, dried milk, fresh milk, grain, bread, potatoes, vegetables, fruit, total diet, drinking water, and human bone. Furthermore, 90Sr was determined in local samples of air, rain water, grass, sea plants, fish, and meat. Caesium-137 was determined in soil, sea water, milk, grain products, potatoes, vegetables, fruit, total diet, fish, and meat. It was also measured by wholebody-counting of a control group at Tis\u00f8. Estimates of the mean contents of radiostrontium and radiocaesium in the human diet in Denmark during 1974 are given. The gamma-background was measured regularly at locations around Ris\u00f8, at ten of the State experimental farms, in one area in Zealand, one in Jutland, and along the shores of the Great Belt. Finally the report includes routine surveys of environmental samples from the Ris\u00f8 area.", "contents": "Environmental Radioactivity in Denmark in 1974. The present report deals with the measurement of fall-out radioactivity in Denmark in 1974. Strontium-90 was determined in samples from all over the country of precipitation, soil, ground water, sea water, grass, dried milk, fresh milk, grain, bread, potatoes, vegetables, fruit, total diet, drinking water, and human bone. Furthermore, 90Sr was determined in local samples of air, rain water, grass, sea plants, fish, and meat. Caesium-137 was determined in soil, sea water, milk, grain products, potatoes, vegetables, fruit, total diet, fish, and meat. It was also measured by wholebody-counting of a control group at Tis\u00f8. Estimates of the mean contents of radiostrontium and radiocaesium in the human diet in Denmark during 1974 are given. The gamma-background was measured regularly at locations around Ris\u00f8, at ten of the State experimental farms, in one area in Zealand, one in Jutland, and along the shores of the Great Belt. Finally the report includes routine surveys of environmental samples from the Ris\u00f8 area."}
{"id": "PMID:1083057", "title": "Environmental Radioactivity in the Faroes in 1974.", "content": "Measurements of fall-out radioactivity in the Faroes in 1974 are presented. Strontium-90 (and 137Cs in most cases) was determined in regularly collected samples of precipitation, grass, milk, fish, sea water, bread, and drinking water. In addition, analyses were made of spotsamples of lamb, potatoes, sea plants, vegetables, eggs, and human bone. Estimates are given of the mean contents of 90Sr and 137Cs in the human diet in the Faroes in 1974. Whole body measurements were made on six individuals from the Faroes.", "contents": "Environmental Radioactivity in the Faroes in 1974. Measurements of fall-out radioactivity in the Faroes in 1974 are presented. Strontium-90 (and 137Cs in most cases) was determined in regularly collected samples of precipitation, grass, milk, fish, sea water, bread, and drinking water. In addition, analyses were made of spotsamples of lamb, potatoes, sea plants, vegetables, eggs, and human bone. Estimates are given of the mean contents of 90Sr and 137Cs in the human diet in the Faroes in 1974. Whole body measurements were made on six individuals from the Faroes."}
{"id": "PMID:1083058", "title": "Environmental radioactivity in Greenland in 1974.", "content": "Measurements of fall-out radioactivity in Greenland in 1974 are reported. Strontium-90 (and Caesium-137 in most cases) was determined in samples of precipitation, sea water, vegetation, animals, and drinking water. Estimates are given of the mean contents of 90Sr and 137Cs in the human diet in Greenland in 1974. Three Greenlanders were measured by wholebody counting.", "contents": "Environmental radioactivity in Greenland in 1974. Measurements of fall-out radioactivity in Greenland in 1974 are reported. Strontium-90 (and Caesium-137 in most cases) was determined in samples of precipitation, sea water, vegetation, animals, and drinking water. Estimates are given of the mean contents of 90Sr and 137Cs in the human diet in Greenland in 1974. Three Greenlanders were measured by wholebody counting."}
{"id": "PMID:1083059", "title": "[Demonstration of Australia antigen in a rheumatologic population].", "content": "The authors looked for Australia antigen in a population of 578 rheumatic patients, 300 of whom were polyarthritic, by electrosyneresis and complement fixation methods. Australia antigen was found in 26 patients, and anti-Australia antibodies in 33 patients. Most of these patients were suffering from rheumatoid polyarthritis--22 presented hepatic disturbance, and 28 had undergone surgical operations, blood transfusions, and repeated hospitalization. The presence of Australia antigen did not in any case appear to play a role in the development of the lesions, apart from the occurrence of signs of hepatic lesions, but appeared to be an indicator of a form of hospitalization.", "contents": "[Demonstration of Australia antigen in a rheumatologic population]. The authors looked for Australia antigen in a population of 578 rheumatic patients, 300 of whom were polyarthritic, by electrosyneresis and complement fixation methods. Australia antigen was found in 26 patients, and anti-Australia antibodies in 33 patients. Most of these patients were suffering from rheumatoid polyarthritis--22 presented hepatic disturbance, and 28 had undergone surgical operations, blood transfusions, and repeated hospitalization. The presence of Australia antigen did not in any case appear to play a role in the development of the lesions, apart from the occurrence of signs of hepatic lesions, but appeared to be an indicator of a form of hospitalization."}
{"id": "PMID:1083061", "title": "Electroimmunoassay of alpha1-antitrypsin with high precision.", "content": "A modification of the electroimmunoassay of alpha1-antitrypsin is described. A fixed amount of the antigen is removed by addition of antibody to standards and samples before elctrophoresis. By changing the dilution procedure, the whole area of the gel plate is used for measurements within a small concentration interval. The measurement range can be changed by the addition of different amounts of antibody. The precision is increased about three fold, with a coefficient of variation within series of 0.6% to 1.2% and between series of 0.6% to 0.7%. This improvement in precision can be used to increase the significance of measured differences in alpha1-antitrypsin concentrations. The possibility of obtaining the same precision by an increased number of replicate analyses by the normal electroimmunoassay is discussed.", "contents": "Electroimmunoassay of alpha1-antitrypsin with high precision. A modification of the electroimmunoassay of alpha1-antitrypsin is described. A fixed amount of the antigen is removed by addition of antibody to standards and samples before elctrophoresis. By changing the dilution procedure, the whole area of the gel plate is used for measurements within a small concentration interval. The measurement range can be changed by the addition of different amounts of antibody. The precision is increased about three fold, with a coefficient of variation within series of 0.6% to 1.2% and between series of 0.6% to 0.7%. This improvement in precision can be used to increase the significance of measured differences in alpha1-antitrypsin concentrations. The possibility of obtaining the same precision by an increased number of replicate analyses by the normal electroimmunoassay is discussed."}
{"id": "PMID:1083062", "title": "Acquired von Willebrand's syndrome and thrombopathy in a patient with chronic lymphocytic leukaemia.", "content": "This paper reports the biological data found during an acquired bleeding disorder occurring in a 65-year-old woman affected with chronic lymphocytic leukaemia. They consists of haemostatic abnormalities which resemble an presently undescribed association of an acquired von Willebrand's syndrome with a thrombopathy. von Willebrand abnormalities were temporarily corrected by infusion of normal cryoprecipitate and reproduced in vitro by the incubation of normal platelets with the patient's IgA. The platelet defect was partially corrected after corticotherapy. The possible relationship of the associated defects is discussed.", "contents": "Acquired von Willebrand's syndrome and thrombopathy in a patient with chronic lymphocytic leukaemia. This paper reports the biological data found during an acquired bleeding disorder occurring in a 65-year-old woman affected with chronic lymphocytic leukaemia. They consists of haemostatic abnormalities which resemble an presently undescribed association of an acquired von Willebrand's syndrome with a thrombopathy. von Willebrand abnormalities were temporarily corrected by infusion of normal cryoprecipitate and reproduced in vitro by the incubation of normal platelets with the patient's IgA. The platelet defect was partially corrected after corticotherapy. The possible relationship of the associated defects is discussed."}
{"id": "PMID:1083067", "title": "Pulmonary circulation in alpha1-antitrypsin deficiency investigated by cinedensigraphy.", "content": "The series consisted of eight cases of severe serum alpha1-antitrypsin deficiency (Piz homozygosity) and eight lung patients with a heterozygous level of the compound. The regional blood pulsation pattern in the lungs in the recumbent position was compared with the pattern in a group of normal controls using a cinedensigraphic device specially constructed for lung circulation studies. In the homozygous group, pulsation in the lower part of the lungs was significantly weakened to the benefit of the apical part. Individual variations occurred. The disturbance observed was not altered with oxygen breathing. This phenomenon is analogous to the redistribution of the blood flow demonstrated by scintigraphic methods. The heterozygous group did not differ significantly from the normal controls with regard to regional pulsation pattern.", "contents": "Pulmonary circulation in alpha1-antitrypsin deficiency investigated by cinedensigraphy. The series consisted of eight cases of severe serum alpha1-antitrypsin deficiency (Piz homozygosity) and eight lung patients with a heterozygous level of the compound. The regional blood pulsation pattern in the lungs in the recumbent position was compared with the pattern in a group of normal controls using a cinedensigraphic device specially constructed for lung circulation studies. In the homozygous group, pulsation in the lower part of the lungs was significantly weakened to the benefit of the apical part. Individual variations occurred. The disturbance observed was not altered with oxygen breathing. This phenomenon is analogous to the redistribution of the blood flow demonstrated by scintigraphic methods. The heterozygous group did not differ significantly from the normal controls with regard to regional pulsation pattern."}
{"id": "PMID:1083068", "title": "Visual tracking and the primate flocculus.", "content": "Purkinje cells in the primate flocculus discharge specifically in relation to visual tracking, effectively generating a velocity profile of the target during pursuit. It is suggested that these neurons supply oculomotor centers with the velocity command signals needed to support pursuit eye movements.", "contents": "Visual tracking and the primate flocculus. Purkinje cells in the primate flocculus discharge specifically in relation to visual tracking, effectively generating a velocity profile of the target during pursuit. It is suggested that these neurons supply oculomotor centers with the velocity command signals needed to support pursuit eye movements."}
{"id": "PMID:1083070", "title": "A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.", "content": "Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes.", "contents": "A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization. Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes."}
{"id": "PMID:1083071", "title": "Carbohydrate deficiency of the factor VIII/von Willebrand factor Protein in von Willebrand's disease variants.", "content": "Study of the normal human factor VIII/von Willebrand factor reveals a macromolecular glycoprotein composed of apparently identical subunits. This purified glycoprotein has procoagulant, antigen, and von Willebrand factor activities. In three patients with a variant of the von Willebrand's disease syndrome, their factor VIII/von Willebrand factor protein was present in normal amounts and had normal procoagulant and antigen activities; however, this protein was deficient in both carbohydrate and von Willebrand factor activity. The carbohydrate portion of the factor VIII/von Willebrand factor glycoprotein is of major importance in its interactions with platelets or the blood vessel wall, or both.", "contents": "Carbohydrate deficiency of the factor VIII/von Willebrand factor Protein in von Willebrand's disease variants. Study of the normal human factor VIII/von Willebrand factor reveals a macromolecular glycoprotein composed of apparently identical subunits. This purified glycoprotein has procoagulant, antigen, and von Willebrand factor activities. In three patients with a variant of the von Willebrand's disease syndrome, their factor VIII/von Willebrand factor protein was present in normal amounts and had normal procoagulant and antigen activities; however, this protein was deficient in both carbohydrate and von Willebrand factor activity. The carbohydrate portion of the factor VIII/von Willebrand factor glycoprotein is of major importance in its interactions with platelets or the blood vessel wall, or both."}
{"id": "PMID:1083075", "title": "In vitro lymphocyte reactivity and T cell levels in patients with melanoma: correlations with clinical and pathological stage.", "content": "In vitro lymphocyte reactivity (LR) to phytohemagglutinin (PHA) and peripheral blood thymus-dependent lymphocyte (T cell) levels were determined in 42 tumor-bearing patients with clinically operable melanoma and were compared to 41 age-matched normal controls. Patients with tumors clinically confined to the primary site (Stage I) as a group had normal immune reactivity and T cell levels, and those with regional metastases by clinical assessment (Stage II) had relatively impaired LR and T cell levels. In six of 24 patients with clinical Stage II tumors, widespread metastases (Stage III) subsequently were found. The severe immune defects in this group with occult disseminated melanoma accounted for the impaired LR and low T cell levels in the group with clinical Stage II tumors. Although overlapping levels of LR and T cells in the patients with pathological Stage II and III tumors prevent use of the data as a determinant of tumor extent in individual patients, the results show that these in vitro assays define a relation between cellular immunocompetence and tumor burden in patients with melanoma.", "contents": "In vitro lymphocyte reactivity and T cell levels in patients with melanoma: correlations with clinical and pathological stage. In vitro lymphocyte reactivity (LR) to phytohemagglutinin (PHA) and peripheral blood thymus-dependent lymphocyte (T cell) levels were determined in 42 tumor-bearing patients with clinically operable melanoma and were compared to 41 age-matched normal controls. Patients with tumors clinically confined to the primary site (Stage I) as a group had normal immune reactivity and T cell levels, and those with regional metastases by clinical assessment (Stage II) had relatively impaired LR and T cell levels. In six of 24 patients with clinical Stage II tumors, widespread metastases (Stage III) subsequently were found. The severe immune defects in this group with occult disseminated melanoma accounted for the impaired LR and low T cell levels in the group with clinical Stage II tumors. Although overlapping levels of LR and T cells in the patients with pathological Stage II and III tumors prevent use of the data as a determinant of tumor extent in individual patients, the results show that these in vitro assays define a relation between cellular immunocompetence and tumor burden in patients with melanoma."}
{"id": "PMID:1083077", "title": "Suppressor activity of the human spleen and thymus.", "content": "Both the spleen and thymus of man contain a population of cells which can suppress the mixed lymphocyte reaction. Splenic cells suppress the MLC 58 +/- 4.8 percent, and the thymus is able to suppress the MLC 90 +/- 2.6 percent. However, splenic cells require stimulation by vegetable mitogens before suppressor activity can be observed, and the thymus displays spontaneous suppressor activity without prior stimulation. The suppressor effect is linearly related to the log of the cell dose (r = 0.8, p less than 0.01), and at low doses the suppressor cells stimulate rather than inhibit the MLC. The cells are exquisitely sensitive to immunosuppressive drugs, and blood levels encountered in clinical organ transplantation abrogate their suppressive activity. The suppressive activity of the cells is nonspecific, and they are able to inhibit the MLC between individuals totally unrelated to the spleen or thymus donor. Since these cells likely play a role in the development of tolerance, further characterization of their properties is required.", "contents": "Suppressor activity of the human spleen and thymus. Both the spleen and thymus of man contain a population of cells which can suppress the mixed lymphocyte reaction. Splenic cells suppress the MLC 58 +/- 4.8 percent, and the thymus is able to suppress the MLC 90 +/- 2.6 percent. However, splenic cells require stimulation by vegetable mitogens before suppressor activity can be observed, and the thymus displays spontaneous suppressor activity without prior stimulation. The suppressor effect is linearly related to the log of the cell dose (r = 0.8, p less than 0.01), and at low doses the suppressor cells stimulate rather than inhibit the MLC. The cells are exquisitely sensitive to immunosuppressive drugs, and blood levels encountered in clinical organ transplantation abrogate their suppressive activity. The suppressive activity of the cells is nonspecific, and they are able to inhibit the MLC between individuals totally unrelated to the spleen or thymus donor. Since these cells likely play a role in the development of tolerance, further characterization of their properties is required."}
{"id": "PMID:1083078", "title": "Gastric infarction after therapeutic embolization.", "content": "A case of extensive gastric necrosis after therapeutic transcatheter embolization of the left gastric artery with fragments of gelatin sponge for recurrent massive upper gastrointestinal hemorrhage is reported. Although future modifications in technique and in choice of embolic agents undoubtedly will be forthcoming, postembolic ischemic necrosis may prove to be a major limitation of this technique. Until more widespread experience is accumulated and the frequency of postembolic ischemic necrosis is known, it would seem prudent to reserve this procedure for those patients presenting prohibitive surgical risks.", "contents": "Gastric infarction after therapeutic embolization. A case of extensive gastric necrosis after therapeutic transcatheter embolization of the left gastric artery with fragments of gelatin sponge for recurrent massive upper gastrointestinal hemorrhage is reported. Although future modifications in technique and in choice of embolic agents undoubtedly will be forthcoming, postembolic ischemic necrosis may prove to be a major limitation of this technique. Until more widespread experience is accumulated and the frequency of postembolic ischemic necrosis is known, it would seem prudent to reserve this procedure for those patients presenting prohibitive surgical risks."}
{"id": "PMID:1083083", "title": "[Reconstruction of the diaphragm with a pericardial flap after radical pleuropneumonectomy for malignant pleural mesothelioma (author's transl)].", "content": "Pleuropneunomectomy with resection of the diaphragm only offers acceptable therapeutic results in patients with multifocal malignant mesothelioma of the pleura. Diaphragmatic replacement on the right side can be satisfactorily achieved by fixation of the liver to the thoracic wall. On the left side many homologous and heterologous tissues and prosthetic adjungts have been suggested with varying success. This report describes technique and results in two patients where the broad pedicled left hemipericard was successfully used to replace the resected left diaphragm.", "contents": "[Reconstruction of the diaphragm with a pericardial flap after radical pleuropneumonectomy for malignant pleural mesothelioma (author's transl)]. Pleuropneunomectomy with resection of the diaphragm only offers acceptable therapeutic results in patients with multifocal malignant mesothelioma of the pleura. Diaphragmatic replacement on the right side can be satisfactorily achieved by fixation of the liver to the thoracic wall. On the left side many homologous and heterologous tissues and prosthetic adjungts have been suggested with varying success. This report describes technique and results in two patients where the broad pedicled left hemipericard was successfully used to replace the resected left diaphragm."}
{"id": "PMID:1083084", "title": "[Complications after thoraco-retroperitoneal nephrectomy (author's transl)].", "content": "We are of the opinion that for large kidney tumors in the upper region as the afflicted venous portions, the most advantageous incision is a thoraco-retro- peritoneal one. It allows the surgeon total freedom of movement and ensures reliable radical tumor-nephrectomy, as well as reconstruction possibilities in the large vessels. Of course, use of this incision is not without complications. They appear, however, not as often as opponents of this method would suppose. There are other statistics to support our convictions (4). From the check-ups of our thoraco-retroperitoneal nephrectomy patients, we are able to surmise the following: - The post operative mortality rate is not increased with this method. The cause for the mortalities are, as far as one can tell, old age and/or a generally poor condition piror to nephrectomy. - Post operative complications due to access though the thorax are very few. Not one patient had to be re-operated, punctured, or treated using suction drainage. No respiratory difficulties were observed. - Post operative complications from the wound were naturally relative to the extent of the incision. It was also here unnecessary to perform a second operation except in one case of a retroperitoneal hematoma from heparinization. This complication was not the result of this particular incision.", "contents": "[Complications after thoraco-retroperitoneal nephrectomy (author's transl)]. We are of the opinion that for large kidney tumors in the upper region as the afflicted venous portions, the most advantageous incision is a thoraco-retro- peritoneal one. It allows the surgeon total freedom of movement and ensures reliable radical tumor-nephrectomy, as well as reconstruction possibilities in the large vessels. Of course, use of this incision is not without complications. They appear, however, not as often as opponents of this method would suppose. There are other statistics to support our convictions (4). From the check-ups of our thoraco-retroperitoneal nephrectomy patients, we are able to surmise the following: - The post operative mortality rate is not increased with this method. The cause for the mortalities are, as far as one can tell, old age and/or a generally poor condition piror to nephrectomy. - Post operative complications due to access though the thorax are very few. Not one patient had to be re-operated, punctured, or treated using suction drainage. No respiratory difficulties were observed. - Post operative complications from the wound were naturally relative to the extent of the incision. It was also here unnecessary to perform a second operation except in one case of a retroperitoneal hematoma from heparinization. This complication was not the result of this particular incision."}
{"id": "PMID:1083085", "title": "[Pulmonary glomangiosis (author's transl)].", "content": "The clinical aspects, differential diagnosis and aetiology of the rare pulmonary glomangiosis are described by means of four cases observed in our clinic. It is a hamartogenous stenosing disease of the lungvessels, appearing predominately in children who suffer from congenital heart disease. It leads to pulmonary hypertension. The pulmonary glomangiosis is of practical importance: By palliative operation as the banding of the pulmonary artery the demand hypertension in the pulmonary circulation is suppressed artificially, so that in heavy cases a sufficient oxygenation is not provided, and the children die in the postoperative period. From the surgical aspect such a disease of the pulmonary vessels has to be supposed when the blood pressure distally of the experimentally banded pulmonary artery drops insufficiently. The authors recommend to exclude the disease of the vessels by excicion of lungtissue and immediate histologic investigation before the final banding is performed.", "contents": "[Pulmonary glomangiosis (author's transl)]. The clinical aspects, differential diagnosis and aetiology of the rare pulmonary glomangiosis are described by means of four cases observed in our clinic. It is a hamartogenous stenosing disease of the lungvessels, appearing predominately in children who suffer from congenital heart disease. It leads to pulmonary hypertension. The pulmonary glomangiosis is of practical importance: By palliative operation as the banding of the pulmonary artery the demand hypertension in the pulmonary circulation is suppressed artificially, so that in heavy cases a sufficient oxygenation is not provided, and the children die in the postoperative period. From the surgical aspect such a disease of the pulmonary vessels has to be supposed when the blood pressure distally of the experimentally banded pulmonary artery drops insufficiently. The authors recommend to exclude the disease of the vessels by excicion of lungtissue and immediate histologic investigation before the final banding is performed."}
{"id": "PMID:1083086", "title": "[Meningial hemangiopericytoma with two-term late metastasis in lung and thoracic wall (author's transl)].", "content": "A case of two-term thoraco-pulmonary late metastasis after a meningial hemangiopericytoma which had been treated surgically 16 years before is described. Histological features, biological behavior, and problems of late metastasis and of surgical treatment of metastasis are discussed.", "contents": "[Meningial hemangiopericytoma with two-term late metastasis in lung and thoracic wall (author's transl)]. A case of two-term thoraco-pulmonary late metastasis after a meningial hemangiopericytoma which had been treated surgically 16 years before is described. Histological features, biological behavior, and problems of late metastasis and of surgical treatment of metastasis are discussed."}
{"id": "PMID:1083087", "title": "[Secondary isophageal reconstruction after resection of a cardia carcinoma (author's transl)].", "content": "Following abdomino-thoracical total gastrectomy and partial resection of the esophagus performed because of the presence of a stomac cancer located in the upper part of the organ an attempt was made to execute from a left sided thoracotomy an esophago-jejunostomy. During which it was found that the previously prepared loop of the jejunum was too short. The execution of a colon interposition in this particular operational state would have been too demanding on the patient; therefore the operation was concluded with a blind closure of the distal end of the esophagus and a jejunostomy (discussion of the resultant problems included in paper). The reconstruction of the digestive tract by retrosternal colon interposition a few weeks after the resection of the cancer proceeded without complication and with consequently excellent functional effect.", "contents": "[Secondary isophageal reconstruction after resection of a cardia carcinoma (author's transl)]. Following abdomino-thoracical total gastrectomy and partial resection of the esophagus performed because of the presence of a stomac cancer located in the upper part of the organ an attempt was made to execute from a left sided thoracotomy an esophago-jejunostomy. During which it was found that the previously prepared loop of the jejunum was too short. The execution of a colon interposition in this particular operational state would have been too demanding on the patient; therefore the operation was concluded with a blind closure of the distal end of the esophagus and a jejunostomy (discussion of the resultant problems included in paper). The reconstruction of the digestive tract by retrosternal colon interposition a few weeks after the resection of the cancer proceeded without complication and with consequently excellent functional effect."}
{"id": "PMID:1083088", "title": "[Esophagopleural fistula associated with postprandial syncopes. A late complication after pneumonectomy (author's transl)].", "content": "A case of esophagopleural fistula in a 54-year-old man who underwent pneumonectomy for bronchogenic carcinoma is described. As uncommon and hitherto unknown complications postprandial syncopes and a letal arrosion of the right cardiac atrium occurred. The pathogenesis of the esophagopleural fistula, the clinical features, and the pathophysiological mechanism of the swallow syncope are discussed.", "contents": "[Esophagopleural fistula associated with postprandial syncopes. A late complication after pneumonectomy (author's transl)]. A case of esophagopleural fistula in a 54-year-old man who underwent pneumonectomy for bronchogenic carcinoma is described. As uncommon and hitherto unknown complications postprandial syncopes and a letal arrosion of the right cardiac atrium occurred. The pathogenesis of the esophagopleural fistula, the clinical features, and the pathophysiological mechanism of the swallow syncope are discussed."}
{"id": "PMID:1083089", "title": "[Diagnostical and therapeutical aspects of post-traumatical endobronchial bleeding (author's transl)].", "content": "Post-traumatic endobronchial bleeding is a serious concomitant injury in thoracic traumas. Untreated cases are threatened early with pulmonary insufficiency caused by the displacement of a blood clot with in the respiratory tract. Pathophysiology, diagnosis and treatment of the post-traumatic endobronchial bleeding are discussed. Conservative and surgical measures are discussed on the basis of 15 observations. The prognosis of the endobronchial bleeding depends decisevly on the length of its existence and intensity. Conservative or operative procedure should be decided on the basis of repeated endoscopical controls. A liberal attitude towards the operative therapy is recomended.", "contents": "[Diagnostical and therapeutical aspects of post-traumatical endobronchial bleeding (author's transl)]. Post-traumatic endobronchial bleeding is a serious concomitant injury in thoracic traumas. Untreated cases are threatened early with pulmonary insufficiency caused by the displacement of a blood clot with in the respiratory tract. Pathophysiology, diagnosis and treatment of the post-traumatic endobronchial bleeding are discussed. Conservative and surgical measures are discussed on the basis of 15 observations. The prognosis of the endobronchial bleeding depends decisevly on the length of its existence and intensity. Conservative or operative procedure should be decided on the basis of repeated endoscopical controls. A liberal attitude towards the operative therapy is recomended."}
{"id": "PMID:1083090", "title": "[A different approach in the treatment of aneurysms of the ascending aorta (author's transl)].", "content": "A safe method in therapy of the ascending aortic aneurysm by partial resection and reconstruction combined with reinforcement by a nylon net is described. Seven patients were operated upon without hospital mortality. Early operation is recommended, when there is only slight aortic insufficiency. In such a situation it is possible to reinforce the ascending aorta without ECC, to stabilize the progress of widening the ascending aorta and the aortic root.", "contents": "[A different approach in the treatment of aneurysms of the ascending aorta (author's transl)]. A safe method in therapy of the ascending aortic aneurysm by partial resection and reconstruction combined with reinforcement by a nylon net is described. Seven patients were operated upon without hospital mortality. Early operation is recommended, when there is only slight aortic insufficiency. In such a situation it is possible to reinforce the ascending aorta without ECC, to stabilize the progress of widening the ascending aorta and the aortic root."}
{"id": "PMID:1083091", "title": "[Surgery for atrial septal defect in patients over 40 years of age (author's transl)].", "content": "Between 1961 and 1972, 354 patients with atrial septal defect ware treated surgically. Of these 80 patients were aged over 40 years at the time of operation. The hospital mortality was 5%. 2 of these patients had a increased preoperative pulmonary artery systolic pressure (50-75 mmHg), the other 2 had a mitral or tricuspid incompetence. At late follow-up 82% of patients were symptomfree, 18% had slight clinical signs of breathlesness on exertion. The heart size decreased in 37 of 40 patients, the electrocardiographis signs of right ventricular hypertrophy in 23 patients. There was no influence on preexistant dysrhythmias by the operation. It is concluded that patients with atrial septal defect in this age group benefit from surgical closure of the defect.", "contents": "[Surgery for atrial septal defect in patients over 40 years of age (author's transl)]. Between 1961 and 1972, 354 patients with atrial septal defect ware treated surgically. Of these 80 patients were aged over 40 years at the time of operation. The hospital mortality was 5%. 2 of these patients had a increased preoperative pulmonary artery systolic pressure (50-75 mmHg), the other 2 had a mitral or tricuspid incompetence. At late follow-up 82% of patients were symptomfree, 18% had slight clinical signs of breathlesness on exertion. The heart size decreased in 37 of 40 patients, the electrocardiographis signs of right ventricular hypertrophy in 23 patients. There was no influence on preexistant dysrhythmias by the operation. It is concluded that patients with atrial septal defect in this age group benefit from surgical closure of the defect."}
{"id": "PMID:1083092", "title": "[Ethiology and treatment of pleural empyema after thoracic surgery (author's transl)].", "content": "Between 1961 and 1974 a total of 29 patients have been treated for empyema after lung resection. Pneumonectomy for bronchus carcinoma had been previously performed in 19 patients, partial resection of the lung in ten. In the majority of cases a technical insufficiency was at last partially responsible for appearance of empyema. Differentiation between empyema after pneumonectomy and empyema in patients with residual lung parenchyma has proved to be of advantage. As to predisposing factors, treatment and prognosis of empyema in cases with residual parenchyma is comparable to regular pulmonary empyema. Empyema after pneumectomy however is different in treatment and prognosis. Irrigation of the infected thoracic cavity either by repeated punction and/or by continous through-drainage is helpful and improves the otherwise poor prognosis.", "contents": "[Ethiology and treatment of pleural empyema after thoracic surgery (author's transl)]. Between 1961 and 1974 a total of 29 patients have been treated for empyema after lung resection. Pneumonectomy for bronchus carcinoma had been previously performed in 19 patients, partial resection of the lung in ten. In the majority of cases a technical insufficiency was at last partially responsible for appearance of empyema. Differentiation between empyema after pneumonectomy and empyema in patients with residual lung parenchyma has proved to be of advantage. As to predisposing factors, treatment and prognosis of empyema in cases with residual parenchyma is comparable to regular pulmonary empyema. Empyema after pneumectomy however is different in treatment and prognosis. Irrigation of the infected thoracic cavity either by repeated punction and/or by continous through-drainage is helpful and improves the otherwise poor prognosis."}
{"id": "PMID:1083093", "title": "[Ultrastructure of an atrial myxoma (author's transl)].", "content": "Electron microscopic examination of a surgically removed atrial myxoma showed, besides fibrocytes and fibroblasts, mainly isolated smooth muscle cells arranged in solid cellstrands. They are responsible for collagen-and elastin-synthesis. They are presumed to arise from subendocardial smooth muscle cells and considered to be neoplastic.", "contents": "[Ultrastructure of an atrial myxoma (author's transl)]. Electron microscopic examination of a surgically removed atrial myxoma showed, besides fibrocytes and fibroblasts, mainly isolated smooth muscle cells arranged in solid cellstrands. They are responsible for collagen-and elastin-synthesis. They are presumed to arise from subendocardial smooth muscle cells and considered to be neoplastic."}
{"id": "PMID:1083094", "title": "[Microparticles in heart operations with extracorporal circulation (author's transl)].", "content": "Disturbances of the microcirculation during extracorporal circulation are not yet clearly understood. Many authors think that microembolism is reposible. In two groups of patients with different perfusion techniques we were looking for microparticles with a new constant pressure filtration method using the Agglometer r apparatus. During the by-pass no particles were found. Yet, we found particles in the priming when blood was used, in the suction blood and in special blood samples after contact with the pericardium. In these cases it was always possible to eliminate the particles with special filters. The particles in the suction blood seemed to originate from the extracardial blood portion coming from the pericardium. This portion seemed to be responsible for postoperative complications such as hemolysis and petechia. These complications could not be seen when extracardiac suction blood was eliminated or filtrated.", "contents": "[Microparticles in heart operations with extracorporal circulation (author's transl)]. Disturbances of the microcirculation during extracorporal circulation are not yet clearly understood. Many authors think that microembolism is reposible. In two groups of patients with different perfusion techniques we were looking for microparticles with a new constant pressure filtration method using the Agglometer r apparatus. During the by-pass no particles were found. Yet, we found particles in the priming when blood was used, in the suction blood and in special blood samples after contact with the pericardium. In these cases it was always possible to eliminate the particles with special filters. The particles in the suction blood seemed to originate from the extracardial blood portion coming from the pericardium. This portion seemed to be responsible for postoperative complications such as hemolysis and petechia. These complications could not be seen when extracardiac suction blood was eliminated or filtrated."}
{"id": "PMID:1083095", "title": "[Traumatic h\u00e4molysis of verious heart-lung-machines on oxygenators (author's transl)].", "content": "1250 protocols of heart-lung-machines, taken between 1962-1974, underwent a variance analysis. The results show that rollerpumps with high pumpvolumes (80 ccm) are not significantly lower hemolytic than rollerpumps with a pumpvolume of 40 ccm. The hemolysis stands in direct proportion to the blood flow. Membranoxygenators effect a less traumatisation than screen- and bubbleoxygenators. Furthermore the use of fresh ACD-blood shows a lower hemolysis than hemodilution and older ACD stored blood. Type and age of the vitium have no influence on the traumatic hemolysis. The use of the orthograde perfusion is significantly better than the retrograde perfusion; the use of blood-filters is no cause for traumatic hemolysis. The above mentioned results are in accordance to literature, but the facts cannot be objectivated that easily than in invitro experiments.", "contents": "[Traumatic h\u00e4molysis of verious heart-lung-machines on oxygenators (author's transl)]. 1250 protocols of heart-lung-machines, taken between 1962-1974, underwent a variance analysis. The results show that rollerpumps with high pumpvolumes (80 ccm) are not significantly lower hemolytic than rollerpumps with a pumpvolume of 40 ccm. The hemolysis stands in direct proportion to the blood flow. Membranoxygenators effect a less traumatisation than screen- and bubbleoxygenators. Furthermore the use of fresh ACD-blood shows a lower hemolysis than hemodilution and older ACD stored blood. Type and age of the vitium have no influence on the traumatic hemolysis. The use of the orthograde perfusion is significantly better than the retrograde perfusion; the use of blood-filters is no cause for traumatic hemolysis. The above mentioned results are in accordance to literature, but the facts cannot be objectivated that easily than in invitro experiments."}
{"id": "PMID:1083096", "title": "[Effectiveness of local and parenteral antibiotics in infection of aortic dacron grafts (author's transl)].", "content": "The effectiveness of local and parenteral antibiotic prophylaxis in infections of aortic dacron grafts was tested in animal experiments. In 20 foxhounds Dacron-interpositions, 4 cms in length, were inoculated with 50 million Staph aureus bacteria and then treated in 4 different ways: Nr. 1-5 no antibiotic treatment. Nr. 6-10 local antibiotic (Nebacetin, R) treatment. Nr 11-15 parenteral treatment with Gramaxin (R) i.m. Nr. 16-20 combined Nebacetin (R) and Gramaxin (R) treatment. The untreated dogs developed infection of laparotomy wounds, peritoneum udn prosthesis. Nebacetin gave improved results, whereas Gramaxin led to complete eradication of the infection. It is concluded, that antibiotic prophylaxis should be applied in vascular surgery, whenever alloplastic, homologous or autologous implants are used.", "contents": "[Effectiveness of local and parenteral antibiotics in infection of aortic dacron grafts (author's transl)]. The effectiveness of local and parenteral antibiotic prophylaxis in infections of aortic dacron grafts was tested in animal experiments. In 20 foxhounds Dacron-interpositions, 4 cms in length, were inoculated with 50 million Staph aureus bacteria and then treated in 4 different ways: Nr. 1-5 no antibiotic treatment. Nr. 6-10 local antibiotic (Nebacetin, R) treatment. Nr 11-15 parenteral treatment with Gramaxin (R) i.m. Nr. 16-20 combined Nebacetin (R) and Gramaxin (R) treatment. The untreated dogs developed infection of laparotomy wounds, peritoneum udn prosthesis. Nebacetin gave improved results, whereas Gramaxin led to complete eradication of the infection. It is concluded, that antibiotic prophylaxis should be applied in vascular surgery, whenever alloplastic, homologous or autologous implants are used."}
{"id": "PMID:1083097", "title": "[Acupuncture analgesia for open heart surgery (author's transl)].", "content": "The first clinical results of 100 patients, operated since November 1973 using ECC and acupuncture anesthesia are reported. The method of combined chinese and european procedures of analgesia is described. Advantages and problems of the new method are discussed.", "contents": "[Acupuncture analgesia for open heart surgery (author's transl)]. The first clinical results of 100 patients, operated since November 1973 using ECC and acupuncture anesthesia are reported. The method of combined chinese and european procedures of analgesia is described. Advantages and problems of the new method are discussed."}
{"id": "PMID:1083098", "title": "[Compromised lumen of ectopic left circumflex coronary artery by prosthetic valve (author's transl)].", "content": "Implantation of an aortic valve prosthesis in patient with aberrant left circumflex coronary artery arising from the initial portion of the right coronary artery resulted in compression of this vessel by the prosthetic valve and in pump failure after interruption of cardiopulmonary bypass. Incidence and significance of variations in coronary artery origin and course are mentioned.", "contents": "[Compromised lumen of ectopic left circumflex coronary artery by prosthetic valve (author's transl)]. Implantation of an aortic valve prosthesis in patient with aberrant left circumflex coronary artery arising from the initial portion of the right coronary artery resulted in compression of this vessel by the prosthetic valve and in pump failure after interruption of cardiopulmonary bypass. Incidence and significance of variations in coronary artery origin and course are mentioned."}
{"id": "PMID:1083100", "title": "Release and retention of labelled DNA in the thymus and spleen of irradiated mice.", "content": "The process of release and retention of labelled DNA in thymus and spleen of normal and irradiated (60Co) mice has been studied after administration of 3H-thymidine. The results indicate that the dividing fraction of lymphoid cells is more resistant to radiation than the fraction of nondividing lymphocytes. The time courses of the specific activities of DNA in thymus and spleen were different especially after irradiation with lethal doses. It is suggested that the process of depletion in the lymphoid series is probably similar for both thymus and spleen but, the different cellular composition of these organs led to apparently unrelated results.", "contents": "Release and retention of labelled DNA in the thymus and spleen of irradiated mice. The process of release and retention of labelled DNA in thymus and spleen of normal and irradiated (60Co) mice has been studied after administration of 3H-thymidine. The results indicate that the dividing fraction of lymphoid cells is more resistant to radiation than the fraction of nondividing lymphocytes. The time courses of the specific activities of DNA in thymus and spleen were different especially after irradiation with lethal doses. It is suggested that the process of depletion in the lymphoid series is probably similar for both thymus and spleen but, the different cellular composition of these organs led to apparently unrelated results."}
{"id": "PMID:1083121", "title": "[Membrane model in the presentation of surface immunoglobulin on human lymphocytes with peroxidase cabeled antibodies].", "content": "For the demonstration of B-lymphocytes the direct immune peroxydase technique with anti-human IgM was used. In contrast to the biochemical and immunological controls in 11.1% of 30 normal persons positive membrane reactions could be proved without doubt. A certain distinction from other white blood cells and falsely positive contrast reactions is possible by means of counterstain with genuine nuclear red and after contrastation with osmium tetroxyde. The method described has a large number of advantages compared with other methods of the determination of B-cells, which appear usable for the clinician above all on account of the relevance to practice.", "contents": "[Membrane model in the presentation of surface immunoglobulin on human lymphocytes with peroxidase cabeled antibodies]. For the demonstration of B-lymphocytes the direct immune peroxydase technique with anti-human IgM was used. In contrast to the biochemical and immunological controls in 11.1% of 30 normal persons positive membrane reactions could be proved without doubt. A certain distinction from other white blood cells and falsely positive contrast reactions is possible by means of counterstain with genuine nuclear red and after contrastation with osmium tetroxyde. The method described has a large number of advantages compared with other methods of the determination of B-cells, which appear usable for the clinician above all on account of the relevance to practice."}
{"id": "PMID:1083126", "title": "[Rheumatic diseases: a public health problem (author's transl)].", "content": "This study surveys the main quantitative data of the literature concerning the morbidity and the functional and socio-economic influence of rheumatic diseases. The cost of medical care and the functional and socio-economic repercussions entailed by these diseases represent a heavy burden on the community. In France, epidemiological surveys must be carried out, which will include medical attendance rates, sickness incapacity and statistics on disability. Once the amplitude of this problem is determined, it must be communicated to the general public, using sanitary education techniques, and an influence must be exerted on the authorities to undertake a general campaign against rheumatic diseases.", "contents": "[Rheumatic diseases: a public health problem (author's transl)]. This study surveys the main quantitative data of the literature concerning the morbidity and the functional and socio-economic influence of rheumatic diseases. The cost of medical care and the functional and socio-economic repercussions entailed by these diseases represent a heavy burden on the community. In France, epidemiological surveys must be carried out, which will include medical attendance rates, sickness incapacity and statistics on disability. Once the amplitude of this problem is determined, it must be communicated to the general public, using sanitary education techniques, and an influence must be exerted on the authorities to undertake a general campaign against rheumatic diseases."}
{"id": "PMID:1083128", "title": "[Changes in the cytokinetics of Mott's cells following sensitization to BCG under conditions of preliminary treatment of rats with soluble antigens of Mycobacterium tuberculosis].", "content": "The effect of pretreatment with tuberculins on BCG sensitization of rats was expressed in the transformation of precursors into Mott's cells. The tuberculins, particularly the old tuberculin, inhibited the MC-reaction; this seems to be connected with the tolerogenic action of the mycobacterial polysaccharides. An attempt was made to interpret the results from the point of view of the three-component system of the lymphoid cell cooperation during the formation of humoral antibodies, taking into account the competitive antagonistic relations between the delayed hypersensitivity (DH) and the humoral immunity. It postulated that this competition is effectuated through the repression of the B cell function by the T1 lymphocyte killer effectors of the DH committed to the same antigen against which the \"blasts\" or the plasmoblasts (subsequently transformed into MC) were produced. The author believes the latter to be the cells in which specific antiboty synthesis was blocked in the statu nascendi due to ectopic (not in the Golgi zone, but in the cisterns of the rough reticulum) and an excessive complex assembly of polysaccharides with the peptide chains of immunoglobulins.", "contents": "[Changes in the cytokinetics of Mott's cells following sensitization to BCG under conditions of preliminary treatment of rats with soluble antigens of Mycobacterium tuberculosis]. The effect of pretreatment with tuberculins on BCG sensitization of rats was expressed in the transformation of precursors into Mott's cells. The tuberculins, particularly the old tuberculin, inhibited the MC-reaction; this seems to be connected with the tolerogenic action of the mycobacterial polysaccharides. An attempt was made to interpret the results from the point of view of the three-component system of the lymphoid cell cooperation during the formation of humoral antibodies, taking into account the competitive antagonistic relations between the delayed hypersensitivity (DH) and the humoral immunity. It postulated that this competition is effectuated through the repression of the B cell function by the T1 lymphocyte killer effectors of the DH committed to the same antigen against which the \"blasts\" or the plasmoblasts (subsequently transformed into MC) were produced. The author believes the latter to be the cells in which specific antiboty synthesis was blocked in the statu nascendi due to ectopic (not in the Golgi zone, but in the cisterns of the rough reticulum) and an excessive complex assembly of polysaccharides with the peptide chains of immunoglobulins."}
{"id": "PMID:1083130", "title": "[Study of the immunodepressive effect of passive immunization on a model of adaptive immunity].", "content": "The effect of various doses of specific serum administered in passive immunization on the formation of primary and secondary adaptive immunity in mice of the CBA strain was studied. Primary and secondary response was more sensitive to passive immunization on a model of adaptive immunity than in vivo. Most sensitive to the immunodepressive action of specific antibodies IgM and IgG were plaque-forming cells, and most resistant -- rosette-forming cells and cells. This led to a supposition that antibodies administered in passive immunization depressed the development of the immunological response acting at the level of T- and B cells.", "contents": "[Study of the immunodepressive effect of passive immunization on a model of adaptive immunity]. The effect of various doses of specific serum administered in passive immunization on the formation of primary and secondary adaptive immunity in mice of the CBA strain was studied. Primary and secondary response was more sensitive to passive immunization on a model of adaptive immunity than in vivo. Most sensitive to the immunodepressive action of specific antibodies IgM and IgG were plaque-forming cells, and most resistant -- rosette-forming cells and cells. This led to a supposition that antibodies administered in passive immunization depressed the development of the immunological response acting at the level of T- and B cells."}
{"id": "PMID:1083127", "title": "[Stimulation of immunoglobin synthesis and rheumatoid-factor production by rheumatoid synovial effusions and by supernatants of synovial tissue cultures].", "content": "Factors capable of stimulating the synthesis of immunoglobulin by circulating human lymphocytes, as measured by a radioactive immune coprecipitation method, have been demonstrated in rheumatoid synovial eeffusions but not in effusions from patients with other arthritides. The activity of the ffusions was localized in two fractions eluted from an agarose A 5m column: fraction II eluting with IgM and fraction E eluting with albumin. Fraction II enhanced immunoglobulin synthesis only in circulating rheumatoid lymphocytes, while fraction V enhanced immunoglobulin synthesis in both rheumatoid and normal lymphocytes. Similar active fractions were found in culture supernatants of rheumatoid synovial tissue. Enhanced immunoglobulin synthesis was also observed following addition of aggregated IgG to rheumatoid lymphocytes. Finally, increased synthesis of anti-IgG (rheumatoid factor) was observed following addition of fractions II and V to rheumatoid but not to normal lymphocytes. The possible relationship of the active material in fraction II to IgG-containing immune complexes and the active material in fraction V to a T cell produced lymphokine-like mediator of T cell function are discussed. The findings presented provide evidence that in addition to antigenic stimulation, the action of one or more enhancing factors liberated by antigen stimulated mononuclear cells, possibly T lymphocytes, plays a role in the immunoglobulin synthesis observed in rheumatoid synovial tissue.", "contents": "[Stimulation of immunoglobin synthesis and rheumatoid-factor production by rheumatoid synovial effusions and by supernatants of synovial tissue cultures]. Factors capable of stimulating the synthesis of immunoglobulin by circulating human lymphocytes, as measured by a radioactive immune coprecipitation method, have been demonstrated in rheumatoid synovial eeffusions but not in effusions from patients with other arthritides. The activity of the ffusions was localized in two fractions eluted from an agarose A 5m column: fraction II eluting with IgM and fraction E eluting with albumin. Fraction II enhanced immunoglobulin synthesis only in circulating rheumatoid lymphocytes, while fraction V enhanced immunoglobulin synthesis in both rheumatoid and normal lymphocytes. Similar active fractions were found in culture supernatants of rheumatoid synovial tissue. Enhanced immunoglobulin synthesis was also observed following addition of aggregated IgG to rheumatoid lymphocytes. Finally, increased synthesis of anti-IgG (rheumatoid factor) was observed following addition of fractions II and V to rheumatoid but not to normal lymphocytes. The possible relationship of the active material in fraction II to IgG-containing immune complexes and the active material in fraction V to a T cell produced lymphokine-like mediator of T cell function are discussed. The findings presented provide evidence that in addition to antigenic stimulation, the action of one or more enhancing factors liberated by antigen stimulated mononuclear cells, possibly T lymphocytes, plays a role in the immunoglobulin synthesis observed in rheumatoid synovial tissue."}
{"id": "PMID:1083139", "title": "Aortocoronary bypass surgery: Correlation of angiographic symptomatic and functional improvement at 1 year.", "content": "Angiographic changes in the coronary circulation were evaluated in 60 patients 1 year after aortocoronary bypass surgery, and their relation to the postoperative clinical status was examined. Of 124 grafts implanted, 26 were closed, 7 stenotic and 91 (74 percent) patent at 1 year. Progression of occlusive disease occurred in 21 of 57 (37 percent) nongrafted and 78 of 123 (63 percent) grafted vessels. On the basis of location and severity of progression, significant lesions bypassed and patency of grafts, postoperative coronary perfusion was considered optimal in 16 patients (Group I), better in 24 (Group III). Complete freedom from chest pain or lessening of pain (improvement by two New York Heart Association functional classes) occurred in 88 and 79 percent of patients in Group III. Positive preoperative treadmill stress tests became negative after surgery in five of six patients in Group I, five of eight in Grojp II and three of eight in Group III. This study demonstrates that when progression of disease, graft patency and extent of revasculariztion are considered in combination, the postoperative angiographic status of the coronary circulation correlates well with clinical improvement at 1 year. These findings support the hypothesis that improved blood supply to ischemic myocardium is a major factor contributing to relief of angina pectoris after saphenous vein bypass surgery.", "contents": "Aortocoronary bypass surgery: Correlation of angiographic symptomatic and functional improvement at 1 year. Angiographic changes in the coronary circulation were evaluated in 60 patients 1 year after aortocoronary bypass surgery, and their relation to the postoperative clinical status was examined. Of 124 grafts implanted, 26 were closed, 7 stenotic and 91 (74 percent) patent at 1 year. Progression of occlusive disease occurred in 21 of 57 (37 percent) nongrafted and 78 of 123 (63 percent) grafted vessels. On the basis of location and severity of progression, significant lesions bypassed and patency of grafts, postoperative coronary perfusion was considered optimal in 16 patients (Group I), better in 24 (Group III). Complete freedom from chest pain or lessening of pain (improvement by two New York Heart Association functional classes) occurred in 88 and 79 percent of patients in Group III. Positive preoperative treadmill stress tests became negative after surgery in five of six patients in Group I, five of eight in Grojp II and three of eight in Group III. This study demonstrates that when progression of disease, graft patency and extent of revasculariztion are considered in combination, the postoperative angiographic status of the coronary circulation correlates well with clinical improvement at 1 year. These findings support the hypothesis that improved blood supply to ischemic myocardium is a major factor contributing to relief of angina pectoris after saphenous vein bypass surgery."}
{"id": "PMID:1083140", "title": "Chronic carriers of hepatitis B antigen (HBsAg). Histological, biochemical, and immunological findings in 31 voluntary blood donors.", "content": "Among 289 HBsAg carriers detected by the Montreal Red Cross Blood Transfusion Service and seen by our group, 31 submitted voluntarily to liver biopsy. These 31 carriers have now been followed for 10-33 months (mean: 23) and all remained positive for HBsAg. 15 of these 31 subjects had lived in institutions during infancy or childhood and none were drug users. Histological examinations revealed 24 cases of chronic persistent hepatitis (CPH), 2 cases of chronic aggressive hepatitis, 2 with steatosis, and 3 with normal liver. On repeated determination, 16 of the 31 subjects had at least one elevated transaminase level. Transaminases levels could not be correlated with the histological diagnosis. 4 cases had positive antinuclear antibodies, all in the CPH group, a finding that could not be correlated with any clinical, biological, or histological findings. The search for other autoantibodies and the immunoglobulin determinations were totally unrewarding. Thus, it appears that chronic HBsAg carriers in Montreal voluntary blood donors often have chronic hepatitis, usually persistent, occasionally aggressive; liver biopsy still remains the most useful approach in the evaluation of these HBsAg carriers. The HBsAg-carrier state seems to be well tolerated, but further long-term studies are needed to understand the natural history of this condition.", "contents": "Chronic carriers of hepatitis B antigen (HBsAg). Histological, biochemical, and immunological findings in 31 voluntary blood donors. Among 289 HBsAg carriers detected by the Montreal Red Cross Blood Transfusion Service and seen by our group, 31 submitted voluntarily to liver biopsy. These 31 carriers have now been followed for 10-33 months (mean: 23) and all remained positive for HBsAg. 15 of these 31 subjects had lived in institutions during infancy or childhood and none were drug users. Histological examinations revealed 24 cases of chronic persistent hepatitis (CPH), 2 cases of chronic aggressive hepatitis, 2 with steatosis, and 3 with normal liver. On repeated determination, 16 of the 31 subjects had at least one elevated transaminase level. Transaminases levels could not be correlated with the histological diagnosis. 4 cases had positive antinuclear antibodies, all in the CPH group, a finding that could not be correlated with any clinical, biological, or histological findings. The search for other autoantibodies and the immunoglobulin determinations were totally unrewarding. Thus, it appears that chronic HBsAg carriers in Montreal voluntary blood donors often have chronic hepatitis, usually persistent, occasionally aggressive; liver biopsy still remains the most useful approach in the evaluation of these HBsAg carriers. The HBsAg-carrier state seems to be well tolerated, but further long-term studies are needed to understand the natural history of this condition."}
{"id": "PMID:1083141", "title": "Autoimmune factors in African cirrhosis. Correlation with hepatitis B surface antigen and antibody.", "content": "Sera were tested for autoantibodies in 98 patients with cryptogenic cirrhosis in Uganda and results correlated with serological tests for hepatitis B surface antigen (HBs Ag) and antibody to HBs Ag (HBs Ab). Smooth muscle antibodies (SMA) were detected in 23 (24%) of the patients but there was no difference in the incidence of SMA between HBs Ag-positive and negative cases. Antinuclear antibodies (ANA) were detected in five cases; mitochondrial, gastric and thyroid antibodies were not found in any patient. Unlike other geographical locations autoimmune mechanisms appear to play little part in the progression of chronic liver disease in Uganda Africans. Hepatitis B surface antigen was present in 36 (37%) and HBs Ab in 47 (48%) of the patients. Although evidence for past exposure to hepatitis B virus (as shown by detection of HBs Ab) was present in at least 30 out of the 62 HBs Ag-negative cases, there was no greater incidence of autoantibodies in HBs Ag-negative patients with or without HBs Ab. Persistent infection with hepatitis B virus and continuing liver damage may be an important factor but these results do not favor a role for the virus in causing chronic liver disease by triggering off an autoimmune reaction.", "contents": "Autoimmune factors in African cirrhosis. Correlation with hepatitis B surface antigen and antibody. Sera were tested for autoantibodies in 98 patients with cryptogenic cirrhosis in Uganda and results correlated with serological tests for hepatitis B surface antigen (HBs Ag) and antibody to HBs Ag (HBs Ab). Smooth muscle antibodies (SMA) were detected in 23 (24%) of the patients but there was no difference in the incidence of SMA between HBs Ag-positive and negative cases. Antinuclear antibodies (ANA) were detected in five cases; mitochondrial, gastric and thyroid antibodies were not found in any patient. Unlike other geographical locations autoimmune mechanisms appear to play little part in the progression of chronic liver disease in Uganda Africans. Hepatitis B surface antigen was present in 36 (37%) and HBs Ab in 47 (48%) of the patients. Although evidence for past exposure to hepatitis B virus (as shown by detection of HBs Ab) was present in at least 30 out of the 62 HBs Ag-negative cases, there was no greater incidence of autoantibodies in HBs Ag-negative patients with or without HBs Ab. Persistent infection with hepatitis B virus and continuing liver damage may be an important factor but these results do not favor a role for the virus in causing chronic liver disease by triggering off an autoimmune reaction."}
{"id": "PMID:1083142", "title": "Relief of angina pectoris in patients with occluded coronary bypass grafts.", "content": "Thirty-two patients from a larger series of subjects undergoing routine postoperative evaluation of the aortocoronary saphenous bypass graft procedure were found to have one (20 patients) or all (12 patients) of the implanted grafts totally occluded. Such occlusion occurred in association with persistent postoperative subjective improvement manifested by a significant diminution or complete disappearance of angina pectoris. Postoperative myocardial infarction was documented in only seven cases, and the possibility of successful partial revascularization could be logically applied to only 20 of these 32 patients. Other suggested mechanisms for relief of angina pectoris in this setting are mentioned, although none is scientifically proved. It is concluded that (1) the marked subjective improvement documented in this population does not universally correlate with anatomic success and that (2) a diminution in the degree of angina pectoris cannot be clinically applied as a reliable indicator of postoperative graft patency.", "contents": "Relief of angina pectoris in patients with occluded coronary bypass grafts. Thirty-two patients from a larger series of subjects undergoing routine postoperative evaluation of the aortocoronary saphenous bypass graft procedure were found to have one (20 patients) or all (12 patients) of the implanted grafts totally occluded. Such occlusion occurred in association with persistent postoperative subjective improvement manifested by a significant diminution or complete disappearance of angina pectoris. Postoperative myocardial infarction was documented in only seven cases, and the possibility of successful partial revascularization could be logically applied to only 20 of these 32 patients. Other suggested mechanisms for relief of angina pectoris in this setting are mentioned, although none is scientifically proved. It is concluded that (1) the marked subjective improvement documented in this population does not universally correlate with anatomic success and that (2) a diminution in the degree of angina pectoris cannot be clinically applied as a reliable indicator of postoperative graft patency."}
{"id": "PMID:1083143", "title": "Diagnosis of von Willebrand's disease. A comparative study of diagnostic tests on nine families with von Willebrand's disease and its differential diagnosis from hemophilia and thrombocytopathy.", "content": "Nine probands with von Willebrand's disease, and their family members, totalling 43 people, were examined. Twenty-seven had a history of bleeding; 29 had an increased factor VIII activity:factor VIII related antigen ratio; 24 had a decreased factor VIII related antigen; 23 had a prolonged bleeding time; 19 had a reduced platelet adhesiveness; 16 had a decreased factor VIII activity; and 14 had an abnormal ristocetin-induced platelet aggregation. Eight members with both normal beleeding time and normal factor VIII activity were found to have other abnormal tests: elevated ratio of factor VIII activity to factor VIII related antigen in seven; decreased factor VIII related antigen in four; and reduced platelet adhesiveness in one. Therefore, ratio of factor VIII activity to factor VIII related antigen and factor VIII related antigen are more sensitive and may be used for the detection of heterozygous carriers of von Willebrand's disease. Although patients with thrombocytopathy may have a prolonged bleeding time, decreased platelet adhesiveness and reduced platelet aggregation by ristocetin, their factor VIII activity, factor VIII related antigen and ratio of factor VIII activity to factor VIII related antigen are normal and their abnormal ristocetin test cannot be corrected by the addition of factor VIII concentrate. Hemophilic subjects and hemophilic carriers, who are deficient in factor VIII activity, usually have a normal bleeding time, normal platelet adhesiveness, and normal ristocetin test. In contrast to patients with von Willebrand's disease, their factor VIII related antigen is normal or slightly increased and their ratio of factor VIII activity to factor VIII related antigen is significantly reduced. We conclude that ratio of factor VIII activity to factor VIII related antigen and factor VIII related antigen are not only more sensitive but also more specific for the diagnosis of von Willebrand's disease.", "contents": "Diagnosis of von Willebrand's disease. A comparative study of diagnostic tests on nine families with von Willebrand's disease and its differential diagnosis from hemophilia and thrombocytopathy. Nine probands with von Willebrand's disease, and their family members, totalling 43 people, were examined. Twenty-seven had a history of bleeding; 29 had an increased factor VIII activity:factor VIII related antigen ratio; 24 had a decreased factor VIII related antigen; 23 had a prolonged bleeding time; 19 had a reduced platelet adhesiveness; 16 had a decreased factor VIII activity; and 14 had an abnormal ristocetin-induced platelet aggregation. Eight members with both normal beleeding time and normal factor VIII activity were found to have other abnormal tests: elevated ratio of factor VIII activity to factor VIII related antigen in seven; decreased factor VIII related antigen in four; and reduced platelet adhesiveness in one. Therefore, ratio of factor VIII activity to factor VIII related antigen and factor VIII related antigen are more sensitive and may be used for the detection of heterozygous carriers of von Willebrand's disease. Although patients with thrombocytopathy may have a prolonged bleeding time, decreased platelet adhesiveness and reduced platelet aggregation by ristocetin, their factor VIII activity, factor VIII related antigen and ratio of factor VIII activity to factor VIII related antigen are normal and their abnormal ristocetin test cannot be corrected by the addition of factor VIII concentrate. Hemophilic subjects and hemophilic carriers, who are deficient in factor VIII activity, usually have a normal bleeding time, normal platelet adhesiveness, and normal ristocetin test. In contrast to patients with von Willebrand's disease, their factor VIII related antigen is normal or slightly increased and their ratio of factor VIII activity to factor VIII related antigen is significantly reduced. We conclude that ratio of factor VIII activity to factor VIII related antigen and factor VIII related antigen are not only more sensitive but also more specific for the diagnosis of von Willebrand's disease."}
{"id": "PMID:1083144", "title": "Failure of AHF concentration to control bleeding in von Willebrand's disease.", "content": "A newly available dried concentrate of antihemophilic factor (Profilate, Abbott Laboratories) was compared with standard, blood-bank prepared cryoprecipitate in the control of bleeding in a patient with von Willebrand's disease. Profilate effectively raised plasma levels of factor VIII but produced only half the expected increase in plasma ristocetin aggregation factor (RAF), and this RAF did not bind readily to the platelets in the presence of ristocetin. Furthermore, the Profilate had little effect upon the bleeding time or the clinical hemorrhage. In contrast, the cryoprecipitate did increase plasma RAF to the expected level, and this RAF bound readily to the patient's platelets in the presence of ristocetin. Cryoprecipitate promptly controlled bleeding. We conclude that the RAF present in Profilate retains in vitro activity but is incapable of augmenting platelet function in vivo.", "contents": "Failure of AHF concentration to control bleeding in von Willebrand's disease. A newly available dried concentrate of antihemophilic factor (Profilate, Abbott Laboratories) was compared with standard, blood-bank prepared cryoprecipitate in the control of bleeding in a patient with von Willebrand's disease. Profilate effectively raised plasma levels of factor VIII but produced only half the expected increase in plasma ristocetin aggregation factor (RAF), and this RAF did not bind readily to the platelets in the presence of ristocetin. Furthermore, the Profilate had little effect upon the bleeding time or the clinical hemorrhage. In contrast, the cryoprecipitate did increase plasma RAF to the expected level, and this RAF bound readily to the patient's platelets in the presence of ristocetin. Cryoprecipitate promptly controlled bleeding. We conclude that the RAF present in Profilate retains in vitro activity but is incapable of augmenting platelet function in vivo."}
{"id": "PMID:1083146", "title": "Effects of chlorobutanol and bradykinin on myocardial excitation.", "content": "The negative inotropic effect of a commonly used formulation of bradykinin (Sandoz BRS-640) was found to be due to chlorobutanol, a constituent of the preparation. Solutions containing up to 100 mug of crystalline bradykinin/ml had no effect on tension or action-potential shape. Chlorobutanol (500 mug/ml) caused a 30% decrease in contraction amplitude and a 20% increase in action-potential duration. Chlorobutanol lowered conduction velocity and induced conduction failure and automaticity within isolated ventricular muscle strips. Chlorobutanol affected neither positive nor negative treppe. We conclude that bradykinin has no direct action on toad, frog, or rat myocardium. However, chlorobutanol does have direct effects on myocardial cells, acting on the cell membrane and decreasing isometric tension produced by the heart.", "contents": "Effects of chlorobutanol and bradykinin on myocardial excitation. The negative inotropic effect of a commonly used formulation of bradykinin (Sandoz BRS-640) was found to be due to chlorobutanol, a constituent of the preparation. Solutions containing up to 100 mug of crystalline bradykinin/ml had no effect on tension or action-potential shape. Chlorobutanol (500 mug/ml) caused a 30% decrease in contraction amplitude and a 20% increase in action-potential duration. Chlorobutanol lowered conduction velocity and induced conduction failure and automaticity within isolated ventricular muscle strips. Chlorobutanol affected neither positive nor negative treppe. We conclude that bradykinin has no direct action on toad, frog, or rat myocardium. However, chlorobutanol does have direct effects on myocardial cells, acting on the cell membrane and decreasing isometric tension produced by the heart."}
{"id": "PMID:1083149", "title": "Inheritance of acute anterior uveitis and HL-A antigen 27.", "content": "A family study on the inheritance of HL-A antigens and susceptibility to acute anterior uveitis is reported. In the 4 families examined, the antigen 27 (haplotype 2.27) occurred in all of the 7 patients with acute anterior uveitis and in 22 of the 33 healthy family members examined. It is suggested that in Finns the haplotype 2,27 is linked with genes predisposing the carrier to acute anterior uveitis.", "contents": "Inheritance of acute anterior uveitis and HL-A antigen 27. A family study on the inheritance of HL-A antigens and susceptibility to acute anterior uveitis is reported. In the 4 families examined, the antigen 27 (haplotype 2.27) occurred in all of the 7 patients with acute anterior uveitis and in 22 of the 33 healthy family members examined. It is suggested that in Finns the haplotype 2,27 is linked with genes predisposing the carrier to acute anterior uveitis."}
{"id": "PMID:1083150", "title": "[The influence of cortisone upon the metabolism of surviving lens epithelia and iris tissue (author's transl)].", "content": "Warburg experiments showed the oxygen consumption by lens epithelium and iris tissue without and after addition of 4 mg methylprednisolone to 100 cc medium. In the last case the O2-uptake of the epithelium seemed diminished, that of the iris tissue showed significantly reduced to 50%. The determination of the consumption of glucose was done by measuring its level in the medium before and after a 5 day's incubation, without and after addition of 4 mg-% of methylprednisolone, by means of the \"Merckotest\". The consumption of the epithelia lowered to 1/6; contrarily rose that of the iris tissue significantly to the double amount. The metabolism of the iris tissue showed a good adaptation in compensating a lower respiratory activity by a augmented glycolysis, which is impossible to the lens epithelium. This may be one of the factors inducing a cortisonic cataract.", "contents": "[The influence of cortisone upon the metabolism of surviving lens epithelia and iris tissue (author's transl)]. Warburg experiments showed the oxygen consumption by lens epithelium and iris tissue without and after addition of 4 mg methylprednisolone to 100 cc medium. In the last case the O2-uptake of the epithelium seemed diminished, that of the iris tissue showed significantly reduced to 50%. The determination of the consumption of glucose was done by measuring its level in the medium before and after a 5 day's incubation, without and after addition of 4 mg-% of methylprednisolone, by means of the \"Merckotest\". The consumption of the epithelia lowered to 1/6; contrarily rose that of the iris tissue significantly to the double amount. The metabolism of the iris tissue showed a good adaptation in compensating a lower respiratory activity by a augmented glycolysis, which is impossible to the lens epithelium. This may be one of the factors inducing a cortisonic cataract."}
{"id": "PMID:1083151", "title": "[End position nystagmus caused by prolonged lateral gaze (author's transl)].", "content": "Electronystagmograms were recorded from six normal subjects who had to maintain an extreme sideward gaze either in the dark or using a fixation target. End position nystagmus occured in three subjects immediately, in two subjects with a latency of several seconds, and in one subject with a latency of 90 seconds. The nystagmus was typically dissociated and was similar whether the subject viewed a fixation point or was recorded from in darkness.", "contents": "[End position nystagmus caused by prolonged lateral gaze (author's transl)]. Electronystagmograms were recorded from six normal subjects who had to maintain an extreme sideward gaze either in the dark or using a fixation target. End position nystagmus occured in three subjects immediately, in two subjects with a latency of several seconds, and in one subject with a latency of 90 seconds. The nystagmus was typically dissociated and was similar whether the subject viewed a fixation point or was recorded from in darkness."}
{"id": "PMID:1083152", "title": "Ocular involvement in I-cell disease (mucolipidosis II). Light and electron microscopic findings.", "content": "A 5 1/2 year old boy with I-cell disease (mucolipidosis II) had bilateral corneal haziness, early cortical cataracts and bilateral prominence of his eyes associated with shallow bony orbits. He died of pneumonia at age 5 1/2 years. Light and electron microscopic examination of the ocular and orbital tissues revealed an accumulation of acid mucopolysaccharide positive, hyaluronidase resistant material in fibroblasts and histiocytes which had partially replaced Bowman's membrane and the anterior stromal cells of the cornea. Similar material, as well as glycolipid-like substance, was found in the conjunctiva and in the retrobulbar soft tissues.", "contents": "Ocular involvement in I-cell disease (mucolipidosis II). Light and electron microscopic findings. A 5 1/2 year old boy with I-cell disease (mucolipidosis II) had bilateral corneal haziness, early cortical cataracts and bilateral prominence of his eyes associated with shallow bony orbits. He died of pneumonia at age 5 1/2 years. Light and electron microscopic examination of the ocular and orbital tissues revealed an accumulation of acid mucopolysaccharide positive, hyaluronidase resistant material in fibroblasts and histiocytes which had partially replaced Bowman's membrane and the anterior stromal cells of the cornea. Similar material, as well as glycolipid-like substance, was found in the conjunctiva and in the retrobulbar soft tissues."}
{"id": "PMID:1083153", "title": "[Enzymatic studies in lenses of different breeds of mice (author's transl)].", "content": "Leucinaminopeptidase (LAP), lactatedehydrogenase (LDH) and glucose-6-phosphatdehydrogenase (GPDH) were analysed in the lenses of two different breeds of mice. Compared to the lenses of a control stock (Agnes Blum, Jena), in the healthy seeming, heterozygous mice the same activities of LAP, LDH, and GPDH were found. But in the breed with evident microphthalmos and cataract LDH was increased. Possible connections are discused.", "contents": "[Enzymatic studies in lenses of different breeds of mice (author's transl)]. Leucinaminopeptidase (LAP), lactatedehydrogenase (LDH) and glucose-6-phosphatdehydrogenase (GPDH) were analysed in the lenses of two different breeds of mice. Compared to the lenses of a control stock (Agnes Blum, Jena), in the healthy seeming, heterozygous mice the same activities of LAP, LDH, and GPDH were found. But in the breed with evident microphthalmos and cataract LDH was increased. Possible connections are discused."}
{"id": "PMID:1083154", "title": "Measurement of intraocular pressure: a study of its reproducibility.", "content": "Intraocular pressure was measured with Goldmann applanation tonometers by two independent examiners in 420 eyes. A difference between measurements of at least 3 mm Hg occurred in 30% of the eyes. Some evidence suggests that the differences between pressure measuremens were caused by actual changes of intraocular pressure rather than by instrument inaccuracy of technician error.", "contents": "Measurement of intraocular pressure: a study of its reproducibility. Intraocular pressure was measured with Goldmann applanation tonometers by two independent examiners in 420 eyes. A difference between measurements of at least 3 mm Hg occurred in 30% of the eyes. Some evidence suggests that the differences between pressure measuremens were caused by actual changes of intraocular pressure rather than by instrument inaccuracy of technician error."}
{"id": "PMID:1083155", "title": "A method for DC-ERG recording of alert humans.", "content": "We developed a technique for DC-coupled ERG recording of the alert human. The equipment consists of three units: a new cornea suction glass, a separate electrode, and a vacuum control device. We get stable recordings of more than one-half hour. By comparison with the EOG the identity of the EOG \"on\"-peak and ERG c-wave is assured. A fine structure of DC-ERG recording of 100 muV amplitude is closely correlated to the variation of the blood pressure in the ophthalmic artery. It may either be caused by the variable electric conductivity of the eyeball due to the blood pressure or it may reflect a variation of the retinal potential itself.", "contents": "A method for DC-ERG recording of alert humans. We developed a technique for DC-coupled ERG recording of the alert human. The equipment consists of three units: a new cornea suction glass, a separate electrode, and a vacuum control device. We get stable recordings of more than one-half hour. By comparison with the EOG the identity of the EOG \"on\"-peak and ERG c-wave is assured. A fine structure of DC-ERG recording of 100 muV amplitude is closely correlated to the variation of the blood pressure in the ophthalmic artery. It may either be caused by the variable electric conductivity of the eyeball due to the blood pressure or it may reflect a variation of the retinal potential itself."}
{"id": "PMID:1083156", "title": "[Standardized fluorescein injection in serial angiography (author's transl)].", "content": "Maximal initial dye concentration in the retinal arteries is desirable for high quality fluorescein Angiograms and is essential for circulation studies. Such high initial dye concentrations can only be achieved by a very rapid fluorescein injection technique necessitating an automatic injector. A reliable easy to use \"spring-injector\" has been designed for clinical use (Figs. 1 and 2). A disposable syringe is driven by a spring contained in a metal cylinder. Microswitches are activated by the piston of the injector triggering one exposure each at the beginning and at the end of the injection. Time and duration of the injection are therby permanently documented on the film. The \"Terumo Disposable Syringe 10 cc\" is used routinely. By interposing a plastic connector between syringe and injector-piston the injection-volume can be reduced to 5 ml. With diffferent connectors one can inject an volume desired up to 10 ml. The duration of the injection can be varied by using different intravenous catheters. Routinely the \"VYGON Trocaflex catheter Nr. 125.16\" with a needle diameter of 1.1 mm and a length of 30 cm is used resulting in an injection time of 10 ml/1.0 sec or 5 ml/0.5 sec. The largest Trocaflex catheter (No. 125.20) will shorten the injection time to 10 ml/0.5 sec and 5 ml/0.25 sec respectively. Even faster injections can be achieved by shortening the catheter or by using a stronger spring. With the described injector, high-speed, well-standardized fluorescein injections are possible. The intravenous catheter makes parvenous injections impossible and will allow for repeated angiograms even on different days without the need for a new venous puncture.", "contents": "[Standardized fluorescein injection in serial angiography (author's transl)]. Maximal initial dye concentration in the retinal arteries is desirable for high quality fluorescein Angiograms and is essential for circulation studies. Such high initial dye concentrations can only be achieved by a very rapid fluorescein injection technique necessitating an automatic injector. A reliable easy to use \"spring-injector\" has been designed for clinical use (Figs. 1 and 2). A disposable syringe is driven by a spring contained in a metal cylinder. Microswitches are activated by the piston of the injector triggering one exposure each at the beginning and at the end of the injection. Time and duration of the injection are therby permanently documented on the film. The \"Terumo Disposable Syringe 10 cc\" is used routinely. By interposing a plastic connector between syringe and injector-piston the injection-volume can be reduced to 5 ml. With diffferent connectors one can inject an volume desired up to 10 ml. The duration of the injection can be varied by using different intravenous catheters. Routinely the \"VYGON Trocaflex catheter Nr. 125.16\" with a needle diameter of 1.1 mm and a length of 30 cm is used resulting in an injection time of 10 ml/1.0 sec or 5 ml/0.5 sec. The largest Trocaflex catheter (No. 125.20) will shorten the injection time to 10 ml/0.5 sec and 5 ml/0.25 sec respectively. Even faster injections can be achieved by shortening the catheter or by using a stronger spring. With the described injector, high-speed, well-standardized fluorescein injections are possible. The intravenous catheter makes parvenous injections impossible and will allow for repeated angiograms even on different days without the need for a new venous puncture."}
{"id": "PMID:1083157", "title": "[Dendritic lesions in herpes simplex infection of the cornea. Scanning and transmission-electron microscope findings (author's transl)].", "content": "Thirty rabbits were divided into 10 groups of 3 animals. In the case of each animal 1 drop of Herpes virus hominis suspension (HVH) was applied to the intact cornea of the right eye. Within the period between the 2nd and 20th day after infection occurred, three animals were killed each time and the corneae were examined by scanning- and transmission-electron microscope. Circular foci and dendritic lesions were observed starting with the 4th day. After the 12th day, dendritic lesions could no longer be observed. Circular foci and dendritic lesions possess a width of about 200 mu which remains unchanged during the entire observation period. The areas of ulceration are obscured by lateral epithelium migrations. The observation that epithelial lesions do not change their demensions over an extended period of time, speaks for an approximate time balance between cellular damage caused by the viruses and the migratory speed of the epithelium. This mechanism is disrupted in the neighboring circular foci. The cytotoxic effect is predominant. Circular foci are confluent. The dendritic lesion so characteristic for herpes simplex infection of the cornea is explained by the confluence of several circular foci which are accidentally positioned next to each other.", "contents": "[Dendritic lesions in herpes simplex infection of the cornea. Scanning and transmission-electron microscope findings (author's transl)]. Thirty rabbits were divided into 10 groups of 3 animals. In the case of each animal 1 drop of Herpes virus hominis suspension (HVH) was applied to the intact cornea of the right eye. Within the period between the 2nd and 20th day after infection occurred, three animals were killed each time and the corneae were examined by scanning- and transmission-electron microscope. Circular foci and dendritic lesions were observed starting with the 4th day. After the 12th day, dendritic lesions could no longer be observed. Circular foci and dendritic lesions possess a width of about 200 mu which remains unchanged during the entire observation period. The areas of ulceration are obscured by lateral epithelium migrations. The observation that epithelial lesions do not change their demensions over an extended period of time, speaks for an approximate time balance between cellular damage caused by the viruses and the migratory speed of the epithelium. This mechanism is disrupted in the neighboring circular foci. The cytotoxic effect is predominant. Circular foci are confluent. The dendritic lesion so characteristic for herpes simplex infection of the cornea is explained by the confluence of several circular foci which are accidentally positioned next to each other."}
{"id": "PMID:1083158", "title": "Observation of injected fluorescein diffusion after laser treatment of cat fundi. An experimental study with angiography and microscopy.", "content": "Argon laser coagulations with a power of 100 mW and 300 mW, exposure time 0.02 and 0.02 sec, and constant spot size of 100 microns were applied on normal cat fundi. From 1 and 2 hrs to 7 days after the coagulation, the treated eyes were observed angiographically and by fluorescence microscopy after dye injection; freeze dried eyes were examined under a fluorescence microscope. Abnormal dye leakages originated from the chorid and from retinal vessels were detected in the specimens examined within 72 hrs after the coagulation.", "contents": "Observation of injected fluorescein diffusion after laser treatment of cat fundi. An experimental study with angiography and microscopy. Argon laser coagulations with a power of 100 mW and 300 mW, exposure time 0.02 and 0.02 sec, and constant spot size of 100 microns were applied on normal cat fundi. From 1 and 2 hrs to 7 days after the coagulation, the treated eyes were observed angiographically and by fluorescence microscopy after dye injection; freeze dried eyes were examined under a fluorescence microscope. Abnormal dye leakages originated from the chorid and from retinal vessels were detected in the specimens examined within 72 hrs after the coagulation."}
{"id": "PMID:1083159", "title": "[Keratoprosthes made with soft plastic material--first experiments on animals (author's transl)].", "content": "To improve the nutrition of corneal tissue and decrease the extrusion rate in keratoprosthesis we developed implants made with HEMA (hydroxy-ethyl-methacrylate) and inserted these models in rabbit eyes. This soft hydrophilic material seemed to be suitable for intraocular implantation because of its permeability to small molecules, necessary for the nutrition of cornea, and because of its flexibility. Keratoprosthesis with these implants was performed on 30 rabbits. During an observation period of 21 months we did not observe any typical extrusion of the implant. Furthermore it was favorably noted that after insertion of the dehydrated prosthesis the HEMA, enlarged by water absorbtion, developing a close contact to the surrounding corneal tissue. The risk of infection with HEMA keratoprostheses is probably not increased. Further investigations are being performed.", "contents": "[Keratoprosthes made with soft plastic material--first experiments on animals (author's transl)]. To improve the nutrition of corneal tissue and decrease the extrusion rate in keratoprosthesis we developed implants made with HEMA (hydroxy-ethyl-methacrylate) and inserted these models in rabbit eyes. This soft hydrophilic material seemed to be suitable for intraocular implantation because of its permeability to small molecules, necessary for the nutrition of cornea, and because of its flexibility. Keratoprosthesis with these implants was performed on 30 rabbits. During an observation period of 21 months we did not observe any typical extrusion of the implant. Furthermore it was favorably noted that after insertion of the dehydrated prosthesis the HEMA, enlarged by water absorbtion, developing a close contact to the surrounding corneal tissue. The risk of infection with HEMA keratoprostheses is probably not increased. Further investigations are being performed."}
{"id": "PMID:1083161", "title": "[The alteration of visual evoked potentials in light- and dark-adapted states as a measure for the visual field sensibility at elevated intraocular pressure (author's transl)].", "content": "On 12 voluntary subjects with normal visual function the intraocular pressure was increased dependent on the ophthalmic blood pressure Pm = (Ps + Pd)/2. The visual field was tested under this pressure load by means of a subjective method and the visual evoked potential was derived under light and dark adaptation. Comparison of subjective and objective investigations demonstrated that the different visual evoked potentials which are dependent on the state of adaptation are a measure for the pressure sensibility of prelaminar optic nerve fibers.", "contents": "[The alteration of visual evoked potentials in light- and dark-adapted states as a measure for the visual field sensibility at elevated intraocular pressure (author's transl)]. On 12 voluntary subjects with normal visual function the intraocular pressure was increased dependent on the ophthalmic blood pressure Pm = (Ps + Pd)/2. The visual field was tested under this pressure load by means of a subjective method and the visual evoked potential was derived under light and dark adaptation. Comparison of subjective and objective investigations demonstrated that the different visual evoked potentials which are dependent on the state of adaptation are a measure for the pressure sensibility of prelaminar optic nerve fibers."}
{"id": "PMID:1083160", "title": "[Microbiologic Investigations of experimental keratoprostheses made with hydrophilic plastics (author's transl)].", "content": "The subject of our investigations is the question: is there an increased risk of infection with eyes operated on keratoprostheses made with hydrophilic soft plastic material (HEMA)? Our first step was an analysis of the normal conjunctival spectrum of microorganism of our rabbits, operated and nonoperated. Then we made exploratory punctures of the anterior chamber of 7 eyes submitted to implantations of HEMA keratoprostheses. During a period of 10 days this puncture exudate was incubated in a culture medium. In 6 out of 7 cases, even after 10 days of incubation, no microorganisms were found. In one case after 72 hours of incubation, a poor growth of a staphylococcus was observed. It can be assumed that in this case the microorganism entered the anterior chamber during the puncture procedure. The results of these investigations confirm the calculation that the \"pores\" of hydrophilic plastic material are too small for penetration of microorganism.", "contents": "[Microbiologic Investigations of experimental keratoprostheses made with hydrophilic plastics (author's transl)]. The subject of our investigations is the question: is there an increased risk of infection with eyes operated on keratoprostheses made with hydrophilic soft plastic material (HEMA)? Our first step was an analysis of the normal conjunctival spectrum of microorganism of our rabbits, operated and nonoperated. Then we made exploratory punctures of the anterior chamber of 7 eyes submitted to implantations of HEMA keratoprostheses. During a period of 10 days this puncture exudate was incubated in a culture medium. In 6 out of 7 cases, even after 10 days of incubation, no microorganisms were found. In one case after 72 hours of incubation, a poor growth of a staphylococcus was observed. It can be assumed that in this case the microorganism entered the anterior chamber during the puncture procedure. The results of these investigations confirm the calculation that the \"pores\" of hydrophilic plastic material are too small for penetration of microorganism."}
{"id": "PMID:1083162", "title": "[Quantitative determination of antiproteinases in human tears (author's transl)].", "content": "By quantitative determination of antiproteinases, i.e. alpha1-antitrypsin, alpha1-antichymotrypsin, and inter-alpha-trypsin inhibitor normal values could be obtained. The importance of antiproteinases is demonstrated in this paper. Especially alpha1-antitrypsin was very easy to quantitate and might be of diagnostic interest.", "contents": "[Quantitative determination of antiproteinases in human tears (author's transl)]. By quantitative determination of antiproteinases, i.e. alpha1-antitrypsin, alpha1-antichymotrypsin, and inter-alpha-trypsin inhibitor normal values could be obtained. The importance of antiproteinases is demonstrated in this paper. Especially alpha1-antitrypsin was very easy to quantitate and might be of diagnostic interest."}
{"id": "PMID:1083163", "title": "[A contribution for recording data in strabismus (author's transl)].", "content": "The recording of the data of all investigations in strabismus concomitans convergens is done by a formular, which is presented in this paper (Fig. 1). The data code is printed on  the backside (Fig. 2). It is possible to evaluate the results with this formular in a computer by punch cards. A quick information without computer help is possible as well. The methods of recording data used by different authors are explained and the advantages and disadvantages are discussed.", "contents": "[A contribution for recording data in strabismus (author's transl)]. The recording of the data of all investigations in strabismus concomitans convergens is done by a formular, which is presented in this paper (Fig. 1). The data code is printed on  the backside (Fig. 2). It is possible to evaluate the results with this formular in a computer by punch cards. A quick information without computer help is possible as well. The methods of recording data used by different authors are explained and the advantages and disadvantages are discussed."}
{"id": "PMID:1083164", "title": "[Results of surgical treatment in strabismus convergens (author's transl)].", "content": "In 252 patients with strabismus concomitans convergens the surgical method of the combined medial rectus recession and lateral rectus resection (Fig. 1) was studied retrospectively. The reduction of the deviation was compared with the added displacement of both muscles (Figs. 2 and 3) or the displacement of one single muscles (Table 1 and 2). Fig. 4 shows the relation between the recession-resection and the reduction of the deviation. A formula for this reduction (Z) is given: Z=6.7 chi recession (in mm) + 2.2 chi resection (in mm) - 8.1. In long the recession is 3 times more effective than the resection. In 61.5% of the patients the eyes stood parallel after surgery or a residual deviation up to 5 degrees was achieved. There was a little difference in the total results of the first (1 week) and the second (6 months) postoperative control. Major differences occurred when comparing single data.", "contents": "[Results of surgical treatment in strabismus convergens (author's transl)]. In 252 patients with strabismus concomitans convergens the surgical method of the combined medial rectus recession and lateral rectus resection (Fig. 1) was studied retrospectively. The reduction of the deviation was compared with the added displacement of both muscles (Figs. 2 and 3) or the displacement of one single muscles (Table 1 and 2). Fig. 4 shows the relation between the recession-resection and the reduction of the deviation. A formula for this reduction (Z) is given: Z=6.7 chi recession (in mm) + 2.2 chi resection (in mm) - 8.1. In long the recession is 3 times more effective than the resection. In 61.5% of the patients the eyes stood parallel after surgery or a residual deviation up to 5 degrees was achieved. There was a little difference in the total results of the first (1 week) and the second (6 months) postoperative control. Major differences occurred when comparing single data."}
{"id": "PMID:1083165", "title": "HLA-compatible donor cornea for prevention of allograft reaction.", "content": "Our series comprises 80 penetrating grafts. Of the recipient corneas 36 were vascularized. Of the donor corneas 48 were fresh and 32 were cryopreserved. As prophylaxis, we gave topical and systemic prednisolone, combined, in heavily vascularized cases, with azathioprine. Corneal donor-recipient pairs were divided into three groups according to HLA histocompatibility. In Group I  (0-1 mismatch) the rate of allograft reaction was 1 in 27, in Group II (2-4 mismatches) it was 4 in 19, and in the untyped Group III 9 in 34. Even when the prognosis was poor, a well-matched donor cornea (Group I) greatly improved the chances of success; when the corneal bed was vascularized, the well-matched donor corneas had a failure rate of 1 in 13, whereas in untyped pairs it was 7 in 18. The importance of possible presensitization is discussed.", "contents": "HLA-compatible donor cornea for prevention of allograft reaction. Our series comprises 80 penetrating grafts. Of the recipient corneas 36 were vascularized. Of the donor corneas 48 were fresh and 32 were cryopreserved. As prophylaxis, we gave topical and systemic prednisolone, combined, in heavily vascularized cases, with azathioprine. Corneal donor-recipient pairs were divided into three groups according to HLA histocompatibility. In Group I  (0-1 mismatch) the rate of allograft reaction was 1 in 27, in Group II (2-4 mismatches) it was 4 in 19, and in the untyped Group III 9 in 34. Even when the prognosis was poor, a well-matched donor cornea (Group I) greatly improved the chances of success; when the corneal bed was vascularized, the well-matched donor corneas had a failure rate of 1 in 13, whereas in untyped pairs it was 7 in 18. The importance of possible presensitization is discussed."}
{"id": "PMID:1083166", "title": "Glaucomatous cupping of the human optic disk: a neuro-histologic study.", "content": "The optic nerve and optic disk in 20 cases of glaucoma were examined neurohistologically. The interruption of retinal axons was found localized in a narrow transversal strip at the level of the posterior part of the lamina cribrosa and immediately behind it. Retinal axons anterior to this zone were mostly intact as long as their ganglion cells of origin were preserved. This picture was essentially identical in cases of primary and secondary glaucoma. The significance of this finding is discussed together with some hypotheses on the origin and causes of the glaucomatous cupping of the optic disk.", "contents": "Glaucomatous cupping of the human optic disk: a neuro-histologic study. The optic nerve and optic disk in 20 cases of glaucoma were examined neurohistologically. The interruption of retinal axons was found localized in a narrow transversal strip at the level of the posterior part of the lamina cribrosa and immediately behind it. Retinal axons anterior to this zone were mostly intact as long as their ganglion cells of origin were preserved. This picture was essentially identical in cases of primary and secondary glaucoma. The significance of this finding is discussed together with some hypotheses on the origin and causes of the glaucomatous cupping of the optic disk."}
{"id": "PMID:1083167", "title": "The influence of age on the chronatic aberration of the eye.", "content": "The axial chromatic aberration of the eye was measured in 58 persons varying from 10 to 80 years of age. It was found that the chromatic aberration diminished significantly after the onset of presbyopia. It is suggested that this decrease in axial chromatic aberration may be due to increase in the index of refraction of the vitreous thereby producing a negative optical surface in the eye which more or less cancels the chromatic aberration of the cornea and anterior surface of the lens. This supposition is supported by measurements of chromatic aberration made on 10 aphakic patients. It is also observed that at near, older eyes who exhibit very little chromatic aberration, cannot make use of this aberration to spare some of the required accommodation (simulated by ophthalmic lenses in older eyes) as is the case in younger eyes.", "contents": "The influence of age on the chronatic aberration of the eye. The axial chromatic aberration of the eye was measured in 58 persons varying from 10 to 80 years of age. It was found that the chromatic aberration diminished significantly after the onset of presbyopia. It is suggested that this decrease in axial chromatic aberration may be due to increase in the index of refraction of the vitreous thereby producing a negative optical surface in the eye which more or less cancels the chromatic aberration of the cornea and anterior surface of the lens. This supposition is supported by measurements of chromatic aberration made on 10 aphakic patients. It is also observed that at near, older eyes who exhibit very little chromatic aberration, cannot make use of this aberration to spare some of the required accommodation (simulated by ophthalmic lenses in older eyes) as is the case in younger eyes."}
{"id": "PMID:1083168", "title": "[The accuracy of ophthalmodynamometry in the diagnosis of occlusions of the carotid artery (author's transl)].", "content": "The aim of this study was to evaluate the diagnostic accuracy of ophthalmodynamometry in cases of occlusion of the internal carotid artery. Among the 29 patients examined with ODM there were 12 cases of such an occlusion, which were unknown to the ophthalmological examiner. All of them could be diagnosed exactly. When by means of ODM an occlusion in the region of the common or internal carotid was excluded the result was equally accurate. A decrease in ophthalmic blood pressure by 15% or a side difference of the same degree indicated in 80% of the cases an occlusion of the internal carotid. The ODM seems, therefore, to be a suitable test for finding or excluding such an occlusion.", "contents": "[The accuracy of ophthalmodynamometry in the diagnosis of occlusions of the carotid artery (author's transl)]. The aim of this study was to evaluate the diagnostic accuracy of ophthalmodynamometry in cases of occlusion of the internal carotid artery. Among the 29 patients examined with ODM there were 12 cases of such an occlusion, which were unknown to the ophthalmological examiner. All of them could be diagnosed exactly. When by means of ODM an occlusion in the region of the common or internal carotid was excluded the result was equally accurate. A decrease in ophthalmic blood pressure by 15% or a side difference of the same degree indicated in 80% of the cases an occlusion of the internal carotid. The ODM seems, therefore, to be a suitable test for finding or excluding such an occlusion."}
{"id": "PMID:1083169", "title": "[ERG- and EOG results in the vitreo-retinal syndrom of Jaffe (author's transl)].", "content": "An ERG and EOG were performed in 2 cases of Jaffe's vitreo-retinal traction syndrom with differing stages of the disease. In the first stage of the disease there was a crease in the b-waves in the presence of a normal EOG. In stage II there was a beginning leveling of the ERG with gradual disappearance of the x-waves and b1-waves in the presence of the subnormal EOG. In stage III, there was a clear discrepancy between the monophasic ERG with deep a-wave and smooth b-waves, and the subnormal EOG, thus indicating damage to the retinal vascular system which is important for the nutrition of the III neurons.", "contents": "[ERG- and EOG results in the vitreo-retinal syndrom of Jaffe (author's transl)]. An ERG and EOG were performed in 2 cases of Jaffe's vitreo-retinal traction syndrom with differing stages of the disease. In the first stage of the disease there was a crease in the b-waves in the presence of a normal EOG. In stage II there was a beginning leveling of the ERG with gradual disappearance of the x-waves and b1-waves in the presence of the subnormal EOG. In stage III, there was a clear discrepancy between the monophasic ERG with deep a-wave and smooth b-waves, and the subnormal EOG, thus indicating damage to the retinal vascular system which is important for the nutrition of the III neurons."}
{"id": "PMID:1083170", "title": "ERG of humans without C-wave.", "content": "Three out of 18 healthy persons investigated with the DC-ERG do not show a c-wave. By varying the adaptation levels and intensities of the stimuli four components in the human ERG can be isolated, the cone and rod late receptor potentials, the positive DC response, and a c-wave in most subjects. It is suggested that investigation of these components reveals a more detailed understanding of the pathophysiologic mechanisms in some retinal diseases.", "contents": "ERG of humans without C-wave. Three out of 18 healthy persons investigated with the DC-ERG do not show a c-wave. By varying the adaptation levels and intensities of the stimuli four components in the human ERG can be isolated, the cone and rod late receptor potentials, the positive DC response, and a c-wave in most subjects. It is suggested that investigation of these components reveals a more detailed understanding of the pathophysiologic mechanisms in some retinal diseases."}
{"id": "PMID:1083171", "title": "[Measurements of the maximal excursion-ability of the globe (author's transl)].", "content": "Method and results of measurements of the maximal version movements by using a millimeter graduated scale after Kestenbaum are descripted. We prefer a millimeter net scale formed as a spectacle. In this way measurements can be done in an accuracy of +/- 0.5 mm about.", "contents": "[Measurements of the maximal excursion-ability of the globe (author's transl)]. Method and results of measurements of the maximal version movements by using a millimeter graduated scale after Kestenbaum are descripted. We prefer a millimeter net scale formed as a spectacle. In this way measurements can be done in an accuracy of +/- 0.5 mm about."}
{"id": "PMID:1083172", "title": "[Myocardial contractility in dogs in hemorrhagic shock and after volumn replacement (author's transl)].", "content": "Myocardial contractility was evaluated in 8 out of 14 anaesthetized mongrel dogs during haemorrhagic shock and after volume replacement with Dextran 60 using the force-velocity relation of the contractile elements at zero load (Vmax). 7 animals received 50,000 or 20,000 KIE respectively of a proteinase inhibitor after bleeding and immediately before and one hour after the infusion of Dextran 60. The release of the lysosomal enzymes acid phosphatase and beta-glucuronidase was inhibited significantly (p less than 0.05) in the animals treated with Trasylol. However, the inhibition of the lysosomal enzymes seems not to have a decisive influence on the dynamic of the macro- and microcirculation and the myocardial contractility.", "contents": "[Myocardial contractility in dogs in hemorrhagic shock and after volumn replacement (author's transl)]. Myocardial contractility was evaluated in 8 out of 14 anaesthetized mongrel dogs during haemorrhagic shock and after volume replacement with Dextran 60 using the force-velocity relation of the contractile elements at zero load (Vmax). 7 animals received 50,000 or 20,000 KIE respectively of a proteinase inhibitor after bleeding and immediately before and one hour after the infusion of Dextran 60. The release of the lysosomal enzymes acid phosphatase and beta-glucuronidase was inhibited significantly (p less than 0.05) in the animals treated with Trasylol. However, the inhibition of the lysosomal enzymes seems not to have a decisive influence on the dynamic of the macro- and microcirculation and the myocardial contractility."}
{"id": "PMID:1083173", "title": "Influence of adrenalectomy, total body x-irradiation and dexamethasone on the amount of CRF-granules and \"classical\" neurosecretory material in the rat neurohypophysis.", "content": "In 195 female Wistar rats the influence of adrenalectomy, total body X-irradiation and dexamethasone treatment on the amount of CRF-granules in the external zone of the median eminence and \"classical\" neurosecretory material (NSM) in the internal zone of the median and in the posterior lobe of the hypophysis has been studied. In the normal rat only very few CRF-granules occur. 15 days after adrenalectomy increased amounts of CRF-granules but normal amounts of \"classical\" NSM are found. X-irradiation leads to a slight diminishment of \"classical\" NSM in normal rats. In adrenalectomized animals X-irradiation causes no changes in the amount of \"classical\" NSM but a marked decrease in the amount of CRF-granules. Application of dexamethasone from the 15th to 18th day after adrenalectomy enhances the augmentation of CRF-granules in irradiated and non-irradiated rats but does not influence the amount of \"classical\" NSM. The findings show that no correlation exists between the quantitative changes of CRF-granules and \"classical\" NSM under the experimental conditions used. Therefore it is to be assumed that the release of the two substances is regulated by different control mechanisms and that the substances have a different functional significance. The observations do not exclude the possibility that CRF-granules and \"classical\" NSM are biochemically identical.", "contents": "Influence of adrenalectomy, total body x-irradiation and dexamethasone on the amount of CRF-granules and \"classical\" neurosecretory material in the rat neurohypophysis. In 195 female Wistar rats the influence of adrenalectomy, total body X-irradiation and dexamethasone treatment on the amount of CRF-granules in the external zone of the median eminence and \"classical\" neurosecretory material (NSM) in the internal zone of the median and in the posterior lobe of the hypophysis has been studied. In the normal rat only very few CRF-granules occur. 15 days after adrenalectomy increased amounts of CRF-granules but normal amounts of \"classical\" NSM are found. X-irradiation leads to a slight diminishment of \"classical\" NSM in normal rats. In adrenalectomized animals X-irradiation causes no changes in the amount of \"classical\" NSM but a marked decrease in the amount of CRF-granules. Application of dexamethasone from the 15th to 18th day after adrenalectomy enhances the augmentation of CRF-granules in irradiated and non-irradiated rats but does not influence the amount of \"classical\" NSM. The findings show that no correlation exists between the quantitative changes of CRF-granules and \"classical\" NSM under the experimental conditions used. Therefore it is to be assumed that the release of the two substances is regulated by different control mechanisms and that the substances have a different functional significance. The observations do not exclude the possibility that CRF-granules and \"classical\" NSM are biochemically identical."}
{"id": "PMID:1083176", "title": "High prevalence of antinuclear antibodies in sandblasters' silicosis.", "content": "Of 39 silicotic sandblasters evaluated in 1972 and 1973, 17 (44 per cent) had positive serum antinuclear antibody reactions. This prevalence is higher than those reported for other pneumonconioses. Comparison of the groups positive and negative for antinuclear antibodies showed that patients positive for antinuclear antibodies were unlikely to have normal ventilatory function and were more likely to have roentgenograms showing large opacities. Both groups had similar proportions showing lobar cavitation or contraction.", "contents": "High prevalence of antinuclear antibodies in sandblasters' silicosis. Of 39 silicotic sandblasters evaluated in 1972 and 1973, 17 (44 per cent) had positive serum antinuclear antibody reactions. This prevalence is higher than those reported for other pneumonconioses. Comparison of the groups positive and negative for antinuclear antibodies showed that patients positive for antinuclear antibodies were unlikely to have normal ventilatory function and were more likely to have roentgenograms showing large opacities. Both groups had similar proportions showing lobar cavitation or contraction."}
{"id": "PMID:1083174", "title": "Incidence of prolonged pain relief following acupuncture.", "content": "From March 1973 to Decenber 1974, 2090 electroacupuncture treatments were applied to 533 patients with chronic pain. Of 533 patients, 276 (52%) reported excellent (greater than 75%) pain relief immediately after the last treatment, and 103 (19.3%) still reported excellent pain relief on a 4-week followup questionnaire. In March 1975, the authors contacted 87 of these 103 patients. Sixty-three reported that they still had excellent pain relief 3 to 18 months after therapy, 3 patients reported that their pain still was relieved 50%, and 21 patients said their pain had returned to the same intensity as before therapy. Thus, 12% of 533 patients who received acupuncture treatment for chronic pain had a significant degree of pain relief at least 3 months after therapy.", "contents": "Incidence of prolonged pain relief following acupuncture. From March 1973 to Decenber 1974, 2090 electroacupuncture treatments were applied to 533 patients with chronic pain. Of 533 patients, 276 (52%) reported excellent (greater than 75%) pain relief immediately after the last treatment, and 103 (19.3%) still reported excellent pain relief on a 4-week followup questionnaire. In March 1975, the authors contacted 87 of these 103 patients. Sixty-three reported that they still had excellent pain relief 3 to 18 months after therapy, 3 patients reported that their pain still was relieved 50%, and 21 patients said their pain had returned to the same intensity as before therapy. Thus, 12% of 533 patients who received acupuncture treatment for chronic pain had a significant degree of pain relief at least 3 months after therapy."}
{"id": "PMID:1083179", "title": "Some dielectric properties of muscle membrane and their possible importance for excitation-contraction coupling.", "content": "Voltage-clamp studies of polarization currents have shown the presence of polar molecules in the membrane of skeletal muscle. Their high dipole moment and favorable transition potential suggest that they may be \"gating molecules\" which serve to regulate some membrane potential-dependent physiological function. Experiments with the drugs tetrodotoxin and tetracaine show that these polar molecules are unlikely to play a role in regulating the sodium and potassium channels responsible for electrical excitation. Instead, they may regulate calcium release from the sarcoplasmic reticulum, which also depends steeply on the cell membrane potential. Schneider and Chandler's hypothesis whereby calcium release from the sarcoplasmic reticulum is regulated by voltage-sensitive \"gating molecules\" in the tubule membrane is still unproven, but remains attractive and plausible.", "contents": "Some dielectric properties of muscle membrane and their possible importance for excitation-contraction coupling. Voltage-clamp studies of polarization currents have shown the presence of polar molecules in the membrane of skeletal muscle. Their high dipole moment and favorable transition potential suggest that they may be \"gating molecules\" which serve to regulate some membrane potential-dependent physiological function. Experiments with the drugs tetrodotoxin and tetracaine show that these polar molecules are unlikely to play a role in regulating the sodium and potassium channels responsible for electrical excitation. Instead, they may regulate calcium release from the sarcoplasmic reticulum, which also depends steeply on the cell membrane potential. Schneider and Chandler's hypothesis whereby calcium release from the sarcoplasmic reticulum is regulated by voltage-sensitive \"gating molecules\" in the tubule membrane is still unproven, but remains attractive and plausible."}
{"id": "PMID:1083180", "title": "Some electronystagmographic manifestations of central nervous system disease.", "content": "A battery of tests with electronystagmography were performed on two groups of patients, one in which posterior fossa abnormalities had been confirmed independently by neurologic findings, contrast studies, or surgery; the other group consisted of what seemed clinically clear cut peripheral VIII nerve or labyrinthine disturbances. The two groups were compared statistically for the incidence of factors thought to indicate the presence of brain stem lesions. No single factor is absolutely reliable by itself as an indicator of brain stem disease, but the use of a test battery permits a much more accurate indication of probability of the presence of brain stem lesions.", "contents": "Some electronystagmographic manifestations of central nervous system disease. A battery of tests with electronystagmography were performed on two groups of patients, one in which posterior fossa abnormalities had been confirmed independently by neurologic findings, contrast studies, or surgery; the other group consisted of what seemed clinically clear cut peripheral VIII nerve or labyrinthine disturbances. The two groups were compared statistically for the incidence of factors thought to indicate the presence of brain stem lesions. No single factor is absolutely reliable by itself as an indicator of brain stem disease, but the use of a test battery permits a much more accurate indication of probability of the presence of brain stem lesions."}
{"id": "PMID:1083181", "title": "Vestibular decruitment in central nervous system disease.", "content": "The differential caloric test consisting of a weak and of a strong stimulation may provide evidence of certain vestibulo-oculomotor abnormality. The phenomenon of vestibular decruitment serves as an indication of central nervous system or intracranial pathology. A classification of the degree of decruitment was attempted in 139 patients exhibiting this phenomenon. Ample evidence is provided that vestibular decruitment is indicative of a variety of intracranial lesions predominantly in the posterior fossa. Decruitment was encountered with hypo- and hyperactive vestibular responses alike and also in patients in whom there was no deficit in end organ sensitivity. The armamentarium of neurotologic testing is enriched by the differential caloric stimulations providing an indication  of the \"site of lesion.\"", "contents": "Vestibular decruitment in central nervous system disease. The differential caloric test consisting of a weak and of a strong stimulation may provide evidence of certain vestibulo-oculomotor abnormality. The phenomenon of vestibular decruitment serves as an indication of central nervous system or intracranial pathology. A classification of the degree of decruitment was attempted in 139 patients exhibiting this phenomenon. Ample evidence is provided that vestibular decruitment is indicative of a variety of intracranial lesions predominantly in the posterior fossa. Decruitment was encountered with hypo- and hyperactive vestibular responses alike and also in patients in whom there was no deficit in end organ sensitivity. The armamentarium of neurotologic testing is enriched by the differential caloric stimulations providing an indication  of the \"site of lesion.\""}
{"id": "PMID:1083183", "title": "Temporal bone histopathology of osteopetrosis.", "content": "The histopathology of the temporal bone of an eight and one-half-year-old girl with osteopetrosis (Albers-Sch\u00f6nberg disease) was studied to evaluate the pathogenesis of its frequent complications in hearing and facial nerve function. The patient was blind and had acute otitis media, but facial paralysis was not noted. Although the size of the temporal bone pyramid was markedly increased due to enormously thickened periosteal layer, changes in the endosteal and endochondral layers  were less marked. Exostotic growth of periosteal bone was seen in the middle ear wall, and the tympanic cavity appeared to be narrowed. At the oval window region, the facial nerve was pushed down towards the stapes due to extreme overgrowth of periosteal bone of the epitympanum, and the superstructure of the stapes was imbedded deeply into the dislocated facial nerve and had strongly compressed it. Although the footplate was free from ankylosis, the crus of the stapes appeared to be immobilized because it was lodged in the facial nerve. Such changes appeared to be the pathogenesis of one form of facial nerve paralysis and conductive hearing loss associated with osteopetrosis.", "contents": "Temporal bone histopathology of osteopetrosis. The histopathology of the temporal bone of an eight and one-half-year-old girl with osteopetrosis (Albers-Sch\u00f6nberg disease) was studied to evaluate the pathogenesis of its frequent complications in hearing and facial nerve function. The patient was blind and had acute otitis media, but facial paralysis was not noted. Although the size of the temporal bone pyramid was markedly increased due to enormously thickened periosteal layer, changes in the endosteal and endochondral layers  were less marked. Exostotic growth of periosteal bone was seen in the middle ear wall, and the tympanic cavity appeared to be narrowed. At the oval window region, the facial nerve was pushed down towards the stapes due to extreme overgrowth of periosteal bone of the epitympanum, and the superstructure of the stapes was imbedded deeply into the dislocated facial nerve and had strongly compressed it. Although the footplate was free from ankylosis, the crus of the stapes appeared to be immobilized because it was lodged in the facial nerve. Such changes appeared to be the pathogenesis of one form of facial nerve paralysis and conductive hearing loss associated with osteopetrosis."}
{"id": "PMID:1083188", "title": "[Trisomy 10q24--10qter].", "content": "The syndrome of trisomy 10q24leads to 10qter is described from three patients studied by the authors and five cases reported from the literature. The characteristic dysmorphy of the syndrome, although different from that of trisomy 21, nevertheless is reminiscent because of euroasiatic facies. The facies is broad, round, because of euroasiatic facies. The facies is broad, round, and flat, with a prominent malar region and a high and convex forehead. Palpebral fissures are narrow with bilateral epicanthal folds, and the nasal bridge is hypoplasitc. Marked joint laxity allows spontaneous and reductible subluxations. Growth retardation is the rule, and mental retardation is severe. No specific dermatoglyphic abnormalities are noted; there is a gap between the first and second toes, with a marked plantar crease separating them. Although trisomy 10q24leads to10qter results from various terminal translocations or pericentric inversion in one case, the location of the breakage point in band 10q24 suggests a particular lability of this zone.", "contents": "[Trisomy 10q24--10qter]. The syndrome of trisomy 10q24leads to 10qter is described from three patients studied by the authors and five cases reported from the literature. The characteristic dysmorphy of the syndrome, although different from that of trisomy 21, nevertheless is reminiscent because of euroasiatic facies. The facies is broad, round, because of euroasiatic facies. The facies is broad, round, and flat, with a prominent malar region and a high and convex forehead. Palpebral fissures are narrow with bilateral epicanthal folds, and the nasal bridge is hypoplasitc. Marked joint laxity allows spontaneous and reductible subluxations. Growth retardation is the rule, and mental retardation is severe. No specific dermatoglyphic abnormalities are noted; there is a gap between the first and second toes, with a marked plantar crease separating them. Although trisomy 10q24leads to10qter results from various terminal translocations or pericentric inversion in one case, the location of the breakage point in band 10q24 suggests a particular lability of this zone."}
{"id": "PMID:1083189", "title": "[Prevalence of etinoblastoma in the Midi-Pyren\u00e9es area].", "content": "The frequency of retinoblastoma was estimated in southwest France (Midi-Pyr\u00e9n\u00e9es) during the 1950-1969 period. Twenty-three cases were detected among 507 157 liveborns. After correcting for the probability of ascertainment of patient, the estimated frequency is 1 per 15 849 births. The proportion of unilateral cases is estimated 91 percent after correcting for the higher probability of ascertaining bilateral cases.", "contents": "[Prevalence of etinoblastoma in the Midi-Pyren\u00e9es area]. The frequency of retinoblastoma was estimated in southwest France (Midi-Pyr\u00e9n\u00e9es) during the 1950-1969 period. Twenty-three cases were detected among 507 157 liveborns. After correcting for the probability of ascertainment of patient, the estimated frequency is 1 per 15 849 births. The proportion of unilateral cases is estimated 91 percent after correcting for the higher probability of ascertaining bilateral cases."}
{"id": "PMID:1083190", "title": "[Pericentric inversion of no. 3, homozygous and heterozygous, and centromeric transposition of no. 12 in a family of orangutans. Implications for evolution].", "content": "An orang-utan family is reported in which the father (Piku) is homozygous for a pericentric inversion of chromosome 3 and heterozygous for a structural rearrangement of chromosome 12 (author's nomenclature for this species). The latter is interpreted as transposition of the centromere by insertion into band q213 [ins cen(12)(q213)]. Piku's mate has a normal female chromosome complement. His daughters by this mate (Agn\u00e8s and Ursula) are heterozygous for both his rearrangements. A fifth orang-utan (Arnold) captured on a different Malesian island was found to have one chromosome 12 similar to that of Piku, and to have a female complement, even though his keapers considered him male. Several hypotheses are proposed to explain the observations, including consanguinity, the existence of a common polymorphism in the species, and the existence of an established subspecies among orang-utans.", "contents": "[Pericentric inversion of no. 3, homozygous and heterozygous, and centromeric transposition of no. 12 in a family of orangutans. Implications for evolution]. An orang-utan family is reported in which the father (Piku) is homozygous for a pericentric inversion of chromosome 3 and heterozygous for a structural rearrangement of chromosome 12 (author's nomenclature for this species). The latter is interpreted as transposition of the centromere by insertion into band q213 [ins cen(12)(q213)]. Piku's mate has a normal female chromosome complement. His daughters by this mate (Agn\u00e8s and Ursula) are heterozygous for both his rearrangements. A fifth orang-utan (Arnold) captured on a different Malesian island was found to have one chromosome 12 similar to that of Piku, and to have a female complement, even though his keapers considered him male. Several hypotheses are proposed to explain the observations, including consanguinity, the existence of a common polymorphism in the species, and the existence of an established subspecies among orang-utans."}
{"id": "PMID:1083191", "title": "Identification of isochromosome 17 in a girl with mental retardation and congenital malformations.", "content": "An isochromosome 17 was found in all analyzed cells of a mentally retarded and malformed girl. As the abnormal chromosome displayed a polymorphic distal band also observed in chromosome 17 of the father, it is suggested that the abnormal chromosome is the result of a non sister chromatid interchange at the level of the centromeric region of chromosomes 17 in paternal meiosis.", "contents": "Identification of isochromosome 17 in a girl with mental retardation and congenital malformations. An isochromosome 17 was found in all analyzed cells of a mentally retarded and malformed girl. As the abnormal chromosome displayed a polymorphic distal band also observed in chromosome 17 of the father, it is suggested that the abnormal chromosome is the result of a non sister chromatid interchange at the level of the centromeric region of chromosomes 17 in paternal meiosis."}
{"id": "PMID:1083192", "title": "Chromosome band analysis in 19 cases of chronic myeloid leukemia: 9 chronic, 10 blastic, two with Ph1 (22q-) translocation on 17 short arm.", "content": "The results of Giemsa band analyses of the marrow cells of 19 cases of CML in the chronic or blast phase are reported. All but one of the 14 Ph1 positive cases showed the 9q+pattern, the exceptional one being 16p+. All 10 blast cases showed additional rearrangements, which are described. In two of the cases, one blastic, one chronic, which were -22, Ph1 negative, the 22q- member was identified as a translocation on the short arm of one 17 which was dicentric.", "contents": "Chromosome band analysis in 19 cases of chronic myeloid leukemia: 9 chronic, 10 blastic, two with Ph1 (22q-) translocation on 17 short arm. The results of Giemsa band analyses of the marrow cells of 19 cases of CML in the chronic or blast phase are reported. All but one of the 14 Ph1 positive cases showed the 9q+pattern, the exceptional one being 16p+. All 10 blast cases showed additional rearrangements, which are described. In two of the cases, one blastic, one chronic, which were -22, Ph1 negative, the 22q- member was identified as a translocation on the short arm of one 17 which was dicentric."}
{"id": "PMID:1083193", "title": "Coagulation studies in patients with trisomy 8 syndrome.", "content": "Coagulation studies were performed in three patients with trisomy for chromosome No. 8. In contrast to the findings reported by others, all patients were found to have a normal coagulation status without sign of factor VII deficiency.", "contents": "Coagulation studies in patients with trisomy 8 syndrome. Coagulation studies were performed in three patients with trisomy for chromosome No. 8. In contrast to the findings reported by others, all patients were found to have a normal coagulation status without sign of factor VII deficiency."}
{"id": "PMID:1083194", "title": "[A second example of telomeric fusion 2 X chromosomes].", "content": "A translocation which originated by telomeric \"fusion\" of X short arms, t(X;X), was found in a woman with primary amenorrhea and a phenotype corresponding partially to Turner's syndrome. Staining with acridine orange after BrdU incorporation showed the distal segments of both Xp's to be much more modified by the treatment than equivalent segments of normal late replicating X's. This could mean important inactivation of segments usually active even in late replicating X's. This functional monosomy for X would have a clinical effect similar to a true monosomy.", "contents": "[A second example of telomeric fusion 2 X chromosomes]. A translocation which originated by telomeric \"fusion\" of X short arms, t(X;X), was found in a woman with primary amenorrhea and a phenotype corresponding partially to Turner's syndrome. Staining with acridine orange after BrdU incorporation showed the distal segments of both Xp's to be much more modified by the treatment than equivalent segments of normal late replicating X's. This could mean important inactivation of segments usually active even in late replicating X's. This functional monosomy for X would have a clinical effect similar to a true monosomy."}
{"id": "PMID:1083197", "title": "[Partial 11q monosomy and trigonocephaly. A new syndrome].", "content": "Partial monosomy 11q occurring de novo and concerning the 11q231 leads to qter region, is reported in a 2-month-old boy. This observation together with three others from the literature allows the individualization of a syndrome characterized by: severe growth retardation: more or less pronounced mental retardation; trigonocephaly; facial dysmorphia.", "contents": "[Partial 11q monosomy and trigonocephaly. A new syndrome]. Partial monosomy 11q occurring de novo and concerning the 11q231 leads to qter region, is reported in a 2-month-old boy. This observation together with three others from the literature allows the individualization of a syndrome characterized by: severe growth retardation: more or less pronounced mental retardation; trigonocephaly; facial dysmorphia."}
{"id": "PMID:1083198", "title": "Susceptibility of Haemophilus influenzae isolates from blood and cerebrospinal fluid to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole.", "content": "Susceptibility to ampicillin and chloramphenicol in vitro has been determined for Haemophilus influenzae strains isolated from blood and/or cerebrospinal fluid cultures of patients admitted to two Atlanta hospitals from 1 January 1974 to 31 March 1975. Since the appearance of ampicillin-resistant strains of this organism in early 1974, chloramphenicol has been used in these hospitals as initial therapy for severe infection due to H. influenzae. Strains from five of 94 patients were resistant to ampicillin (minimum inhibitory concentration [MIC] >/= 12.5 mug/ml), but all strains were susceptible to chloramphenicol (MIC < 2 mug/ml). The first 35 strains studied, including three resistant to ampicillin, were also tested for in vitro susceptibility to trimethoprim-sulfamethoxazole; all were highly susceptible (MIC </= 0.0312 mug of trimethoprim and 0.625 mug of sulfamethoxazole per ml).", "contents": "Susceptibility of Haemophilus influenzae isolates from blood and cerebrospinal fluid to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole. Susceptibility to ampicillin and chloramphenicol in vitro has been determined for Haemophilus influenzae strains isolated from blood and/or cerebrospinal fluid cultures of patients admitted to two Atlanta hospitals from 1 January 1974 to 31 March 1975. Since the appearance of ampicillin-resistant strains of this organism in early 1974, chloramphenicol has been used in these hospitals as initial therapy for severe infection due to H. influenzae. Strains from five of 94 patients were resistant to ampicillin (minimum inhibitory concentration [MIC] >/= 12.5 mug/ml), but all strains were susceptible to chloramphenicol (MIC < 2 mug/ml). The first 35 strains studied, including three resistant to ampicillin, were also tested for in vitro susceptibility to trimethoprim-sulfamethoxazole; all were highly susceptible (MIC </= 0.0312 mug of trimethoprim and 0.625 mug of sulfamethoxazole per ml)."}
{"id": "PMID:1083199", "title": "Mechanisms of ampicillin resistance in Haemophilus influenzae type B.", "content": "The genetic mechanisms associated with ampicillin resistance in strains of Haemophilus influenzae type b were investigated. In experiments concerned with transfer of total deoxyribonucleic acid in vitro, expression of resistance by wild-type strains occurred at a frequency of about 10%. The minimum inhibitory concentration of ampicillin for the transformed strains was similar to that of the resistant donor strains, and resistance in transformants was associated with acquisition of the ability to produce beta-lactamase. Exposure to 39 mug of acridine per ml for 18 h cured resistance at a frequency of 80%, and there was spontaneous loss of resistance after repeated subculture of some strains. Analysis by cesium chloride-ethidium bromide density gradient centrifugation demonstrated the presence of extrachromosomal deoxyribonucleic acid in the resistant strains, providing further evidence that the resistance factor is plasmid mediated.", "contents": "Mechanisms of ampicillin resistance in Haemophilus influenzae type B. The genetic mechanisms associated with ampicillin resistance in strains of Haemophilus influenzae type b were investigated. In experiments concerned with transfer of total deoxyribonucleic acid in vitro, expression of resistance by wild-type strains occurred at a frequency of about 10%. The minimum inhibitory concentration of ampicillin for the transformed strains was similar to that of the resistant donor strains, and resistance in transformants was associated with acquisition of the ability to produce beta-lactamase. Exposure to 39 mug of acridine per ml for 18 h cured resistance at a frequency of 80%, and there was spontaneous loss of resistance after repeated subculture of some strains. Analysis by cesium chloride-ethidium bromide density gradient centrifugation demonstrated the presence of extrachromosomal deoxyribonucleic acid in the resistant strains, providing further evidence that the resistance factor is plasmid mediated."}
{"id": "PMID:1083200", "title": "Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test.", "content": "Fifty-seven cultures of Haemophilus influenzae were tested for production of beta-lactamase by use of a modified, 1-min test. Only the 24 cultures that were ampicillin resistant, whether type b or not, yielded positive results for the enzyme; all ampicillin-susceptible isolates were uniformly negative. The beta-lactamase procedure is simple to perform and requires only commonly available reagents.", "contents": "Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test. Fifty-seven cultures of Haemophilus influenzae were tested for production of beta-lactamase by use of a modified, 1-min test. Only the 24 cultures that were ampicillin resistant, whether type b or not, yielded positive results for the enzyme; all ampicillin-susceptible isolates were uniformly negative. The beta-lactamase procedure is simple to perform and requires only commonly available reagents."}
{"id": "PMID:1083201", "title": "Spurious ampicillin resistance by testing Haemophilus influenzae with agar containing supplement C.", "content": "Ampicillin resistance (minimal inhibitory concentration >/=10 mug/ml) in the absence of beta-lactamase activity by Haemophilus influenzae was noted in tests performed with Mueller-Hinton agar containing one lot of supplement C. All strains, except five with known resistance due to beta-lactamase activity, were inhibited by 0.6 mug or less of ampicillin per ml of chocolatized blood agar.", "contents": "Spurious ampicillin resistance by testing Haemophilus influenzae with agar containing supplement C. Ampicillin resistance (minimal inhibitory concentration >/=10 mug/ml) in the absence of beta-lactamase activity by Haemophilus influenzae was noted in tests performed with Mueller-Hinton agar containing one lot of supplement C. All strains, except five with known resistance due to beta-lactamase activity, were inhibited by 0.6 mug or less of ampicillin per ml of chocolatized blood agar."}
{"id": "PMID:1083202", "title": "Susceptibility of ampicillin-resistant Haemophilus influenzae to seven penicillins.", "content": "Sixty-seven clinical isolates of Haemophilus influenzae from various sections of the United States, England, and Germany were tested for susceptibility to penicillin, ampicillin, amoxicillin, epicillin, carbenicillin, ticarcillin, and methicillin. Fifty-three of the strains had previously been judged to be ampicillin resistant and 14 had been determined to be ampicillin susceptible. Fifty-two of the 53 resistant strains produced beta-lactamase, but none of the susceptible strains produced it. On the basis of minimal inhibitory concentrations, the most active compounds were ticarcillin and carbenicillin. Whether this greater activity is useful clinically has not been established.", "contents": "Susceptibility of ampicillin-resistant Haemophilus influenzae to seven penicillins. Sixty-seven clinical isolates of Haemophilus influenzae from various sections of the United States, England, and Germany were tested for susceptibility to penicillin, ampicillin, amoxicillin, epicillin, carbenicillin, ticarcillin, and methicillin. Fifty-three of the strains had previously been judged to be ampicillin resistant and 14 had been determined to be ampicillin susceptible. Fifty-two of the 53 resistant strains produced beta-lactamase, but none of the susceptible strains produced it. On the basis of minimal inhibitory concentrations, the most active compounds were ticarcillin and carbenicillin. Whether this greater activity is useful clinically has not been established."}
{"id": "PMID:1083203", "title": "A new species of the genus Bullera derx.", "content": "A new yeast species, Bullera piricola, is described. The three strains studied were all isolated from the pear phylloplane. This species differs from all Bullera species known at present by forming symmetrical as well as asymmetrical ballistospores. The problem of its integration into the genus Bullera Derx is discussed. An amended diagnosis of the genus Bullera is given.", "contents": "A new species of the genus Bullera derx. A new yeast species, Bullera piricola, is described. The three strains studied were all isolated from the pear phylloplane. This species differs from all Bullera species known at present by forming symmetrical as well as asymmetrical ballistospores. The problem of its integration into the genus Bullera Derx is discussed. An amended diagnosis of the genus Bullera is given."}
{"id": "PMID:1083204", "title": "The amino acid pool of Hansenula holstil: characterisation, and changes mediated by environment.", "content": "Amino acid pools extracted from Hansenula holstii grown in continuous culture with either ammonia or nitrate as sole source of nitrogen, under a variety of substrate limitations, were characterised and quantified. Pools from corresponding cultures were shown to be similar in size and composition, regardless of whether ammonia or nitrate was the nitrogen source. Large changes in pools (both quantitative and qualitative) occurred when cultures were grown under different substrate limitations. Such changes were particularly large in glutamate, glutamine, alanine, lysine and arginine; the possible significance of such environment-mediated changes is discussed.", "contents": "The amino acid pool of Hansenula holstil: characterisation, and changes mediated by environment. Amino acid pools extracted from Hansenula holstii grown in continuous culture with either ammonia or nitrate as sole source of nitrogen, under a variety of substrate limitations, were characterised and quantified. Pools from corresponding cultures were shown to be similar in size and composition, regardless of whether ammonia or nitrate was the nitrogen source. Large changes in pools (both quantitative and qualitative) occurred when cultures were grown under different substrate limitations. Such changes were particularly large in glutamate, glutamine, alanine, lysine and arginine; the possible significance of such environment-mediated changes is discussed."}
{"id": "PMID:1083205", "title": "Oxidation of organic C1 compounds by Hyphomicrobium spp.", "content": "Washed cell suspensions of Hyphomicrobium spp. were able to oxidize methanol, formaldehyde and formate. This suggested that enzymes for the oxidation of these compounds were present. The pathway of the oxidation of methanol to carbon dioxide and water has been investigated using cell-free extracts. An ammonium-ion-activated, phenazine methosulphate-linked methanol dehydrogenase was detected. This enzyme has a dual substrate specificity for normal primary alcohols and formaldehyde. It has a high pH optimum for activity of 9.5. The pathway is completed by an NAD-linked formate dehydrogenase. This enzyme is inhibited by low concentrations of potassium cyanide, copper sulphate and hypophosphite.", "contents": "Oxidation of organic C1 compounds by Hyphomicrobium spp. Washed cell suspensions of Hyphomicrobium spp. were able to oxidize methanol, formaldehyde and formate. This suggested that enzymes for the oxidation of these compounds were present. The pathway of the oxidation of methanol to carbon dioxide and water has been investigated using cell-free extracts. An ammonium-ion-activated, phenazine methosulphate-linked methanol dehydrogenase was detected. This enzyme has a dual substrate specificity for normal primary alcohols and formaldehyde. It has a high pH optimum for activity of 9.5. The pathway is completed by an NAD-linked formate dehydrogenase. This enzyme is inhibited by low concentrations of potassium cyanide, copper sulphate and hypophosphite."}
{"id": "PMID:1083206", "title": "Production and characterization of the agarase of Cytoplaga flevensis.", "content": "Cytophaga flevensis produced an inducible agarase which was extracellular under most conditions tested. The effect of cultural conditions on the production of enzyme was studied in batch and continuous culture. In batch culture, production was optimal when Cytophaga flevensis was incubated at 20 C in a mineral medium with agar as the sole carbon source and ammonium nitrate as the nitrogen source at an initial pH of 6.6-7.0. The enzyme appeared to be subject to catabolite repression, since its synthesis was repressed when glucose was added to the medium in batch culture. Furthermore, in continuous culture, enzyme production decreased with increasing growth rate. Extracellular agarase was partially purified and the enzyme preparation obtained was very stable. The enzyme has a molecular weight of 26 000 daltons. It is a beta-agarase which is highly specific for polysaccharides containing neoagarobiose units. The final products of hydrolysis of agarose by the endo-acting enzyme were neoagarotetraose and neoagarobiose. Optimal conditions for its activity were pH 6.3 and 30C. When agarose was used as a substrate, an apparent temperature optimum of 35C was found, due to gelling of the substrate during the assay procedure.", "contents": "Production and characterization of the agarase of Cytoplaga flevensis. Cytophaga flevensis produced an inducible agarase which was extracellular under most conditions tested. The effect of cultural conditions on the production of enzyme was studied in batch and continuous culture. In batch culture, production was optimal when Cytophaga flevensis was incubated at 20 C in a mineral medium with agar as the sole carbon source and ammonium nitrate as the nitrogen source at an initial pH of 6.6-7.0. The enzyme appeared to be subject to catabolite repression, since its synthesis was repressed when glucose was added to the medium in batch culture. Furthermore, in continuous culture, enzyme production decreased with increasing growth rate. Extracellular agarase was partially purified and the enzyme preparation obtained was very stable. The enzyme has a molecular weight of 26 000 daltons. It is a beta-agarase which is highly specific for polysaccharides containing neoagarobiose units. The final products of hydrolysis of agarose by the endo-acting enzyme were neoagarotetraose and neoagarobiose. Optimal conditions for its activity were pH 6.3 and 30C. When agarose was used as a substrate, an apparent temperature optimum of 35C was found, due to gelling of the substrate during the assay procedure."}
{"id": "PMID:1083207", "title": "Oxalate and formate in Alcaligenes and Pseudomonas species.", "content": "Oxalate is metabolized by the glycerate pathway involving glyoxylate carboligase in Alcaligenes LOx and Pseudomonas KOx, and by the serine pathway involving hydroxypyruvate reductase in Ps.MOx and Ps.AM1 (var. 470). Although A.LOx does not grow on formate, stimulation of growth was observed in the presence of amino acids and a few Kreb's cycle intermediates. A.LOx possesses two different mechanisms for the oxidation of formate: (1) the constitutive formate oxidase which is present in the particulate fraction of oxalate-grown and succinate-plus-formate-grown cells; (2) the inducible NAD-linked formate dehydrogenase present in the 100 000 x g supernatant fraction of the cell-free extracts of oxalate-grown cells alone. The two systems occur simultaneously in oxalate-grown cells. The effect of inhibitors on formate oxidase activity and the other enzyme activities of the particulate formate-oxidizing fraction indicate that the oxidation of formate is linked to the respiratory chain.", "contents": "Oxalate and formate in Alcaligenes and Pseudomonas species. Oxalate is metabolized by the glycerate pathway involving glyoxylate carboligase in Alcaligenes LOx and Pseudomonas KOx, and by the serine pathway involving hydroxypyruvate reductase in Ps.MOx and Ps.AM1 (var. 470). Although A.LOx does not grow on formate, stimulation of growth was observed in the presence of amino acids and a few Kreb's cycle intermediates. A.LOx possesses two different mechanisms for the oxidation of formate: (1) the constitutive formate oxidase which is present in the particulate fraction of oxalate-grown and succinate-plus-formate-grown cells; (2) the inducible NAD-linked formate dehydrogenase present in the 100 000 x g supernatant fraction of the cell-free extracts of oxalate-grown cells alone. The two systems occur simultaneously in oxalate-grown cells. The effect of inhibitors on formate oxidase activity and the other enzyme activities of the particulate formate-oxidizing fraction indicate that the oxidation of formate is linked to the respiratory chain."}
{"id": "PMID:1083208", "title": "Microbial degradation of piperonylic acid.", "content": "Several organisms were isolated for their ability to utilize piperonylate as a sole carbon source for growth and a Pseudomonas species (Ps. PP-2) was selected for a study of the degradation of this substrate. Only vanillate, isovanillate, p-hydroxybenzoate and protocatechuate, of several possible catabolities, served as growth and oxidation substrates for the organism. Detailed analysis of the culture fluid from piperonylate-grown cells revealed the presence of vanillate and protocatechuate but isovanillate, p-hydroxybenzoate and m-hydroxybenzoate were not detected. The evidence presented suggests that piperonylate is metabolized first to vanillate by methylenedioxy ring cleavage and next to protocatechuate by direct dimethylation of vanillate.", "contents": "Microbial degradation of piperonylic acid. Several organisms were isolated for their ability to utilize piperonylate as a sole carbon source for growth and a Pseudomonas species (Ps. PP-2) was selected for a study of the degradation of this substrate. Only vanillate, isovanillate, p-hydroxybenzoate and protocatechuate, of several possible catabolities, served as growth and oxidation substrates for the organism. Detailed analysis of the culture fluid from piperonylate-grown cells revealed the presence of vanillate and protocatechuate but isovanillate, p-hydroxybenzoate and m-hydroxybenzoate were not detected. The evidence presented suggests that piperonylate is metabolized first to vanillate by methylenedioxy ring cleavage and next to protocatechuate by direct dimethylation of vanillate."}
{"id": "PMID:1083209", "title": "The role of plant particles, bacteria and cell-free supernatant fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of linoleic acid.", "content": "The role of different fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of the linoleic acid has been investigated by a series of in vitro incubations. Hydrolysis of the trilinolein to free linoleic acid occurs almost wholly in the cell-free supernatant; the liberated linoleic acid in the supernatant can be rapidly adsorbed onto food particles where it is hydrogenated to stearic acid via the C18 trans-11 monoene. Some 25% of the trilinolein added as substrate was taken up by the bacteria and of this a small percentage appeared to be hydrolysed and the free linoleic acid hydrogenated to stearic acid intracellularly. No conclusive evidence was obtained to suggest that this intracellular hydrogenation proceeded by a route other than that which took place on the food particles.", "contents": "The role of plant particles, bacteria and cell-free supernatant fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of linoleic acid. The role of different fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of the linoleic acid has been investigated by a series of in vitro incubations. Hydrolysis of the trilinolein to free linoleic acid occurs almost wholly in the cell-free supernatant; the liberated linoleic acid in the supernatant can be rapidly adsorbed onto food particles where it is hydrogenated to stearic acid via the C18 trans-11 monoene. Some 25% of the trilinolein added as substrate was taken up by the bacteria and of this a small percentage appeared to be hydrolysed and the free linoleic acid hydrogenated to stearic acid intracellularly. No conclusive evidence was obtained to suggest that this intracellular hydrogenation proceeded by a route other than that which took place on the food particles."}
{"id": "PMID:1083210", "title": "Methanobacterium arbophilicum sp.nov. An obligate anaerobe isolated from wetwood of living trees.", "content": "The isolation and characterization of a new methanogenic bacterium, Methanobacterium arbophilicum, is described. Isolation from wetwood enrichment cultures, that were obtained from methane-positive trees, required a medium containing inorganic salts, vitamins, and an atmosphere consisting of an 80:20 mixture of hydrogen-carbon dioxide. Isolates of M. arbophilicum were gram-positive, non-motile short rods that occurred singly, in pairs, or chains. The organism was found to be an autotroph and a strick anaerobe, and to have a pH optimum of 7.5-8.0. The optimal temperature for growth was 30 to 37C, the maximum being 45C and the minimum about 10C. The organism had obligate growth requirements for H2 and CO2, and organic compounds greatly stimulated growth. The generation time in shake flask culture was about 17 hr in mineral salts medium and about 13 hr in complex medium. The DNA base composition was 27.5 mol % GC.", "contents": "Methanobacterium arbophilicum sp.nov. An obligate anaerobe isolated from wetwood of living trees. The isolation and characterization of a new methanogenic bacterium, Methanobacterium arbophilicum, is described. Isolation from wetwood enrichment cultures, that were obtained from methane-positive trees, required a medium containing inorganic salts, vitamins, and an atmosphere consisting of an 80:20 mixture of hydrogen-carbon dioxide. Isolates of M. arbophilicum were gram-positive, non-motile short rods that occurred singly, in pairs, or chains. The organism was found to be an autotroph and a strick anaerobe, and to have a pH optimum of 7.5-8.0. The optimal temperature for growth was 30 to 37C, the maximum being 45C and the minimum about 10C. The organism had obligate growth requirements for H2 and CO2, and organic compounds greatly stimulated growth. The generation time in shake flask culture was about 17 hr in mineral salts medium and about 13 hr in complex medium. The DNA base composition was 27.5 mol % GC."}
{"id": "PMID:1083211", "title": "Characteristics of weak H2S-producing isolates of Pseudomonas putrefaciens from human infections.", "content": "Isolates from human clinical specimens which produced salmon-colored colonies were found to grow at 2C and produce ornthine decarboxylase, DNase, trimethylamine and only slight amounts of H2S. The notably weak ability of such isolates to produce H2S was originally regarded as a negative reaction. The DNA base composition of such isolates was found to range from 48.3 to 49.2 moles % GC. These observations, along with bacteriocin typing, resulted in identification of the isolates as Pseudomonas putrefaciens.", "contents": "Characteristics of weak H2S-producing isolates of Pseudomonas putrefaciens from human infections. Isolates from human clinical specimens which produced salmon-colored colonies were found to grow at 2C and produce ornthine decarboxylase, DNase, trimethylamine and only slight amounts of H2S. The notably weak ability of such isolates to produce H2S was originally regarded as a negative reaction. The DNA base composition of such isolates was found to range from 48.3 to 49.2 moles % GC. These observations, along with bacteriocin typing, resulted in identification of the isolates as Pseudomonas putrefaciens."}
{"id": "PMID:1083212", "title": "Galvanic vestibular stimulation.", "content": "In order to gain insight into the origin of the eye movements provoked by galvanic vestibular stimulation, experiments were conducted in rabbits. The animals were subjected to a small galvanic stimulation and to a vestibular stimulation (rotation and sinusoidally changing linear accelerations). Application of several stimuli at the same time shows that the effects on the eye movements of each stimulus given separately are summated. Electric stimulation provoked vestibular reflexes; a stronger effect was observed in unilaterally and in bilaterally labyrinthectomized rabbits. This suggests that there is not only an impact of the oculomotor system but of the peripheral vestibular organs too. The conclusion is drawn that electric stimulation of the vestibular system gives information on the functioning of the labyrinths and also about the side where a labyrinthine lesion is located.", "contents": "Galvanic vestibular stimulation. In order to gain insight into the origin of the eye movements provoked by galvanic vestibular stimulation, experiments were conducted in rabbits. The animals were subjected to a small galvanic stimulation and to a vestibular stimulation (rotation and sinusoidally changing linear accelerations). Application of several stimuli at the same time shows that the effects on the eye movements of each stimulus given separately are summated. Electric stimulation provoked vestibular reflexes; a stronger effect was observed in unilaterally and in bilaterally labyrinthectomized rabbits. This suggests that there is not only an impact of the oculomotor system but of the peripheral vestibular organs too. The conclusion is drawn that electric stimulation of the vestibular system gives information on the functioning of the labyrinths and also about the side where a labyrinthine lesion is located."}
{"id": "PMID:1083213", "title": "Familial dysequilibrium-diplegia with T-lymphocyte deficiency.", "content": "A second family is described with a combination of defective thymus-dependent immunity and cerebral palsy. The cerebral palsy comprised nonprogressive dysequilibrium and mild spastic diplegia without limb ataxia. This genetic entity of presumed autosomal recessive inheritance is clearly distinguished from ataxia-telangiectasia. Immunological abnormalities should be sought in other familial or unexplained cerebral palsy syndromes.", "contents": "Familial dysequilibrium-diplegia with T-lymphocyte deficiency. A second family is described with a combination of defective thymus-dependent immunity and cerebral palsy. The cerebral palsy comprised nonprogressive dysequilibrium and mild spastic diplegia without limb ataxia. This genetic entity of presumed autosomal recessive inheritance is clearly distinguished from ataxia-telangiectasia. Immunological abnormalities should be sought in other familial or unexplained cerebral palsy syndromes."}
{"id": "PMID:1083214", "title": "Benign rectal ulceration of unknown origin. An unusual cause of rectal bleeding.", "content": "An unusual progressive form of benign rectal ulceration was found in an infant who presented with repeated episodes of rectal bleeding. The ulceration failed to respond to medical treatment but was cured by anterior resection of the rectum. The aetiology is not known.", "contents": "Benign rectal ulceration of unknown origin. An unusual cause of rectal bleeding. An unusual progressive form of benign rectal ulceration was found in an infant who presented with repeated episodes of rectal bleeding. The ulceration failed to respond to medical treatment but was cured by anterior resection of the rectum. The aetiology is not known."}
{"id": "PMID:1083217", "title": "Study of lymphoid cells from inflamed synovial membranes.", "content": "Synovectomy specimens from patients with rheumatoid arthritis were cultured and after 24 hours the nonadherent cells were removed. These were found to include cells with the morphological characteristics of lymphocytes. Using the sheep-cell rosetting test, up to 94% of these cells were found to be T cells, and while T cells could be found in all the supernatant cell populations studied, not all made a mitogen response. None or only a very small number of B cells were found by staining for surface immunoglobulin. The possible roles which T lymphocytes might play in chronic inflammation in the rheumatoid synovium are discussed in relation to experimental work, and factors which attract T cells into areas of nonspecific inflammation are similarly considered in the light of animal experiments.", "contents": "Study of lymphoid cells from inflamed synovial membranes. Synovectomy specimens from patients with rheumatoid arthritis were cultured and after 24 hours the nonadherent cells were removed. These were found to include cells with the morphological characteristics of lymphocytes. Using the sheep-cell rosetting test, up to 94% of these cells were found to be T cells, and while T cells could be found in all the supernatant cell populations studied, not all made a mitogen response. None or only a very small number of B cells were found by staining for surface immunoglobulin. The possible roles which T lymphocytes might play in chronic inflammation in the rheumatoid synovium are discussed in relation to experimental work, and factors which attract T cells into areas of nonspecific inflammation are similarly considered in the light of animal experiments."}
{"id": "PMID:1083218", "title": "Pneumocystis pneumonia: the importance of early open lung biopsy.", "content": "Pulmonary infection due to Pneumocystis carinii is now recognized as the leading cause of death from infection in patients with a hematologic malignancy who are in remission. Effective treatment requires suspicion of the infection in susceptible patients and rapid identification of the organism. In most patients, open lung biopsy performed through a small anterior thoracotomy provides immediate identification or exclusion of the organism, thus allowing treatment of infected patients and avoidance of inappropriate therapy in patients without the disease. We feel that the use of early thoracotomy, in spite of the fact that it exposes these very ill patients to a major surgical procedure and general anesthesia, is ultimately the safest therapeutic course.", "contents": "Pneumocystis pneumonia: the importance of early open lung biopsy. Pulmonary infection due to Pneumocystis carinii is now recognized as the leading cause of death from infection in patients with a hematologic malignancy who are in remission. Effective treatment requires suspicion of the infection in susceptible patients and rapid identification of the organism. In most patients, open lung biopsy performed through a small anterior thoracotomy provides immediate identification or exclusion of the organism, thus allowing treatment of infected patients and avoidance of inappropriate therapy in patients without the disease. We feel that the use of early thoracotomy, in spite of the fact that it exposes these very ill patients to a major surgical procedure and general anesthesia, is ultimately the safest therapeutic course."}
{"id": "PMID:1083219", "title": "Portosystemic shunting in patients with primary biliary cirrhosis: a good risk disease.", "content": "Five patients with primary biliary cirrhosis underwent portosystemic shunting for the control of variceal bleeding. Three procedures were emergencies and two were elective. There was no operative mortality; all patients were followed until the present or until death. One patient is alive 4 years and another, 2 years postoperatively. One patient died 4 years after operation and another died 16 months postoperatively. Another patient survived for 8 years following her shunt and eventually died as a result of a cerebrovascular accident. This group of patients is compared to a larger group undergoing portosystemic shunting because of portal hypertension secondary to other forms of liver disease. The absence of operative mortality and the fact that several of these patients had moderately long postoperative survival despite apparently poor liver function suggest that the usual criteria for the assessment of operative risk are not valid in primary biliary cirrhosis.", "contents": "Portosystemic shunting in patients with primary biliary cirrhosis: a good risk disease. Five patients with primary biliary cirrhosis underwent portosystemic shunting for the control of variceal bleeding. Three procedures were emergencies and two were elective. There was no operative mortality; all patients were followed until the present or until death. One patient is alive 4 years and another, 2 years postoperatively. One patient died 4 years after operation and another died 16 months postoperatively. Another patient survived for 8 years following her shunt and eventually died as a result of a cerebrovascular accident. This group of patients is compared to a larger group undergoing portosystemic shunting because of portal hypertension secondary to other forms of liver disease. The absence of operative mortality and the fact that several of these patients had moderately long postoperative survival despite apparently poor liver function suggest that the usual criteria for the assessment of operative risk are not valid in primary biliary cirrhosis."}
{"id": "PMID:1083220", "title": "Idiopathic late-onset immunoglobulin deficiency with functional T-cell deficiency.", "content": "The common features in our two patients were late onset of infections that are known to complicate both T-cell and B-cell deficiencies, decreased numbers of circulating B-cells with low serum immunoglobulin levels, and normal numbers of circulating T-cells, which were, however, defective in the response to delayed hypersensitivity skin test antigens and to mitogens in vitro. The composite of clinical and immunologic aberrations is consistent with the presence of an immune deficiency involving the B-cell system quantitatively and the T-cell system qualitatively.", "contents": "Idiopathic late-onset immunoglobulin deficiency with functional T-cell deficiency. The common features in our two patients were late onset of infections that are known to complicate both T-cell and B-cell deficiencies, decreased numbers of circulating B-cells with low serum immunoglobulin levels, and normal numbers of circulating T-cells, which were, however, defective in the response to delayed hypersensitivity skin test antigens and to mitogens in vitro. The composite of clinical and immunologic aberrations is consistent with the presence of an immune deficiency involving the B-cell system quantitatively and the T-cell system qualitatively."}
{"id": "PMID:1083221", "title": "Interaction of 6,6'-dithiodinicotinic acid with rat thymocytes pretreated with thiol compounds.", "content": "The number of sulfhydryl groups in the membranes of rat thymocytes were determined, expressed in terms of concentration of 6-mercaptonicotinic acid produced by reduction of 6,6'-dithiodinicotinic acid. The influence of incubation of thymocytes in solutions of L-cysteine, reduced glutathione and dithiothreitol on the number of -SH groups was studied. About twofold higher degree of reduction of 6-6'dithiodinicotinic acid was observed in the reaction with thymocytes incubated previously in 2 mM solution of L-cysteine in comparison with controls. Incubation of thymocytes with dithiothreitol and gluthathione had no effect on the number of -SH groups.", "contents": "Interaction of 6,6'-dithiodinicotinic acid with rat thymocytes pretreated with thiol compounds. The number of sulfhydryl groups in the membranes of rat thymocytes were determined, expressed in terms of concentration of 6-mercaptonicotinic acid produced by reduction of 6,6'-dithiodinicotinic acid. The influence of incubation of thymocytes in solutions of L-cysteine, reduced glutathione and dithiothreitol on the number of -SH groups was studied. About twofold higher degree of reduction of 6-6'dithiodinicotinic acid was observed in the reaction with thymocytes incubated previously in 2 mM solution of L-cysteine in comparison with controls. Incubation of thymocytes with dithiothreitol and gluthathione had no effect on the number of -SH groups."}
{"id": "PMID:1083222", "title": "Activity of nonsteroid anti-inflammatory drugs in immunologically specific and nonspecific cellular responses.", "content": "The influence of nonsteroid anti-inflammatory drugs on immunologic reactions of the humoral type (agglutinins, hemolysins) and of the cellular types (blast test, transplantation test), and on the course of immunologically nonspecific inflammatory reactions(xylene inflammation, pleurisy, granulation test) has been studied. Virtually regardless of chemical structure (indomethacin, mefenamic acid, acetylsalicylic acid, chloroquine), the drugs of this group had no influence on humoral responses, but a distinct influence on the delayed type of cellular responses and on immunologically nonspecific reactions. The investigated drugs had quantitatively varying modes of action, and only chloroquine showed a qualitatively different mode of action. It was concluded that nonsteroid anti-inflammatory drugs ace on a common link in inflammatory processes involving the immunologic system, as well as without such an involvement. The mechanism of their effect was considered to consist in stabilization of cell membranes, inhibition of proliferation and cellular transformation, and inhibition of the lymph node permeability factor (LNPF).", "contents": "Activity of nonsteroid anti-inflammatory drugs in immunologically specific and nonspecific cellular responses. The influence of nonsteroid anti-inflammatory drugs on immunologic reactions of the humoral type (agglutinins, hemolysins) and of the cellular types (blast test, transplantation test), and on the course of immunologically nonspecific inflammatory reactions(xylene inflammation, pleurisy, granulation test) has been studied. Virtually regardless of chemical structure (indomethacin, mefenamic acid, acetylsalicylic acid, chloroquine), the drugs of this group had no influence on humoral responses, but a distinct influence on the delayed type of cellular responses and on immunologically nonspecific reactions. The investigated drugs had quantitatively varying modes of action, and only chloroquine showed a qualitatively different mode of action. It was concluded that nonsteroid anti-inflammatory drugs ace on a common link in inflammatory processes involving the immunologic system, as well as without such an involvement. The mechanism of their effect was considered to consist in stabilization of cell membranes, inhibition of proliferation and cellular transformation, and inhibition of the lymph node permeability factor (LNPF)."}
{"id": "PMID:1083223", "title": "The right to know: giving the patient his medical record.", "content": "Each patient  admitted to the 16-bed Rehabilitation Medicine Service at Medical Center Hospital of Vermont since October 1972 has received a carbon copy of his full Admission and Discharge notes, containing the complete problem list, and for each problem the relevant data, the Assessment and the Plans. The objectives were to improve patient education; to improve the patient's chances to contribute to the planning of his care; and to increase the staff's accountability to the patient. Over a period of seven months, we evaluated the effect of this maneuver for 125 consecutive patients by means of (1) a report on the patient's reactions, completed by a nurse after she reviewed the record with the patient; (2) a report by the physician stating whether he had expurgated the record for patient use, and recording his observations of patient and family reaction; (3) a questionnaire mailed to patients after discharge. Results indicated that patients were generally comfortable about reading the record, found it educational and appreciated the trust implied. No substantial difficulties arose. Few records were expurgated. The staff has accepted this style as crucial to an appropriate sharing of responsibility between themselves and the patients. We conclude that giving the patient his record is a safe and inexpensive aid to the rehabilitation process, and is probably mandated by the changing relationships between professionals and their clients, and by the patient's need to negotiate his own health care in an increasingly complex and mobile society.", "contents": "The right to know: giving the patient his medical record. Each patient  admitted to the 16-bed Rehabilitation Medicine Service at Medical Center Hospital of Vermont since October 1972 has received a carbon copy of his full Admission and Discharge notes, containing the complete problem list, and for each problem the relevant data, the Assessment and the Plans. The objectives were to improve patient education; to improve the patient's chances to contribute to the planning of his care; and to increase the staff's accountability to the patient. Over a period of seven months, we evaluated the effect of this maneuver for 125 consecutive patients by means of (1) a report on the patient's reactions, completed by a nurse after she reviewed the record with the patient; (2) a report by the physician stating whether he had expurgated the record for patient use, and recording his observations of patient and family reaction; (3) a questionnaire mailed to patients after discharge. Results indicated that patients were generally comfortable about reading the record, found it educational and appreciated the trust implied. No substantial difficulties arose. Few records were expurgated. The staff has accepted this style as crucial to an appropriate sharing of responsibility between themselves and the patients. We conclude that giving the patient his record is a safe and inexpensive aid to the rehabilitation process, and is probably mandated by the changing relationships between professionals and their clients, and by the patient's need to negotiate his own health care in an increasingly complex and mobile society."}
{"id": "PMID:1083224", "title": "Thermal injury in von willebrand disease. Management of burn wound bleeding.", "content": "A 51-year-old woman sustained a 40% third-degree burn. After five grafting procedures that were unsuccessful due to uncontrolled bleeding, the diagnosis of von Willebrand disease was established. Skin grafting was subsequently accomplished without difficulty using cryoprecipitate as a source of antihemophilic factor (factor VIII). Serial clinical and laboratory investigations demonstrated that using the level of factor VIII procoagulant activity alone as an in vitro index of hemostasis was insufficient as a guide to the control of pathologic burn wound bleeding in this patient.", "contents": "Thermal injury in von willebrand disease. Management of burn wound bleeding. A 51-year-old woman sustained a 40% third-degree burn. After five grafting procedures that were unsuccessful due to uncontrolled bleeding, the diagnosis of von Willebrand disease was established. Skin grafting was subsequently accomplished without difficulty using cryoprecipitate as a source of antihemophilic factor (factor VIII). Serial clinical and laboratory investigations demonstrated that using the level of factor VIII procoagulant activity alone as an in vitro index of hemostasis was insufficient as a guide to the control of pathologic burn wound bleeding in this patient."}
{"id": "PMID:1083225", "title": "The endoscopic diagnosis of an aortoduodenal fistula.", "content": "An aortoduodenal fistula is a serious complication of aortic reconstructive surgery. This artile describes a case in which the diagnosis of an aortoduodenal fistula was made by upper gastrointestinal endoscopy. The endoscopic finding of a vascular prosthesis within the duodenum allowed immediate surgical intervention and control of the gastrointestinal hemorrhage.", "contents": "The endoscopic diagnosis of an aortoduodenal fistula. An aortoduodenal fistula is a serious complication of aortic reconstructive surgery. This artile describes a case in which the diagnosis of an aortoduodenal fistula was made by upper gastrointestinal endoscopy. The endoscopic finding of a vascular prosthesis within the duodenum allowed immediate surgical intervention and control of the gastrointestinal hemorrhage."}
{"id": "PMID:1083226", "title": "Treatment of reflux esophagitis resulting in massive esophageal bleeding.", "content": "The complications that occur secondary to sliding hiatal hernia are reflux esophagitis with ulceration, stricture formation, and hemorrhage. We have treated seven patients for massive esophageal bleeding secondary to reflux esophagitis. All had endoscopic evidence of reflux esophagitis and a negative work-up for other sources of bleeding. All underwent Nissen fundoplication as the only mode of therapy for the bleeding esophagitis. No patient has bled again; healing of the esophagitis was evident six weeks after operation, as observed by gastrointestinal endoscopy. This operation prevents reflux, has minimal side-effects, and gives consistent results in the hands of the average trained gastrointestinal surgeon.", "contents": "Treatment of reflux esophagitis resulting in massive esophageal bleeding. The complications that occur secondary to sliding hiatal hernia are reflux esophagitis with ulceration, stricture formation, and hemorrhage. We have treated seven patients for massive esophageal bleeding secondary to reflux esophagitis. All had endoscopic evidence of reflux esophagitis and a negative work-up for other sources of bleeding. All underwent Nissen fundoplication as the only mode of therapy for the bleeding esophagitis. No patient has bled again; healing of the esophagitis was evident six weeks after operation, as observed by gastrointestinal endoscopy. This operation prevents reflux, has minimal side-effects, and gives consistent results in the hands of the average trained gastrointestinal surgeon."}
{"id": "PMID:1083227", "title": "Primary duodenal tumors: a diagnostic and therapeutic dilemma.", "content": "The case records of ten patients with benign, three with endocrine, and 19 with malignant duodenal tumors were reviewed. Patients with benign tumors had episodes of bleeding that were recurrent in a number of cases. The endocrine tumors were all gastrinomas; patients had ulcer diathesis associated with the Zollinger-Ellison syndrome. All malignant tumors were symptomatic, with chronic gastrointestinal blood loss or duodenal obstruction. All benign tumors were demonstrated preoperatively, but only ten of 19 malignant tumors were confirmed preoperatively. The data suggest that duodenal tumors masquerade as more common diseases and as a result, their diagnosis and treatment are delayed inordinately. Awareness of this problem is encouraged, and a more aggressive diagnostic effort in selected patients, including fiberoptic endoscopy, is suggested.", "contents": "Primary duodenal tumors: a diagnostic and therapeutic dilemma. The case records of ten patients with benign, three with endocrine, and 19 with malignant duodenal tumors were reviewed. Patients with benign tumors had episodes of bleeding that were recurrent in a number of cases. The endocrine tumors were all gastrinomas; patients had ulcer diathesis associated with the Zollinger-Ellison syndrome. All malignant tumors were symptomatic, with chronic gastrointestinal blood loss or duodenal obstruction. All benign tumors were demonstrated preoperatively, but only ten of 19 malignant tumors were confirmed preoperatively. The data suggest that duodenal tumors masquerade as more common diseases and as a result, their diagnosis and treatment are delayed inordinately. Awareness of this problem is encouraged, and a more aggressive diagnostic effort in selected patients, including fiberoptic endoscopy, is suggested."}
{"id": "PMID:1083228", "title": "Arteriovenous malformations of the gastrointestinal tract.", "content": "During an eight-year period, 17 patients ranging in age from 7 months to 81 years were found to have arteriovenous malformations (AVMs) of the gastrointestinal tract complicated by major hemorrhage. After review of these cases, a clinical classification of AVMs was developed, based on angiographic characteristics, localization, age of the patient, and family history. Type 1 AVMs were solitary, localized lesions within the right side of the colon. They occurred in seven patients 55 years of age or older. None were palpable or visible at operation. Type 2 AVMs occurred in seven patients. They were larger and occasionally visible, most commonly in the small intestine, and probably of congenital origin. Symptoms all began before 50 years of age. Type 3 AVMs were punctate angiomas causing gastrointestinal hemorrhage. They occurred in three patients with the classical findings of hereditary hemorrhagic telangiectasia. The diagnosis of most AVMs can be determined preoperatively only by selective angiographic studies.", "contents": "Arteriovenous malformations of the gastrointestinal tract. During an eight-year period, 17 patients ranging in age from 7 months to 81 years were found to have arteriovenous malformations (AVMs) of the gastrointestinal tract complicated by major hemorrhage. After review of these cases, a clinical classification of AVMs was developed, based on angiographic characteristics, localization, age of the patient, and family history. Type 1 AVMs were solitary, localized lesions within the right side of the colon. They occurred in seven patients 55 years of age or older. None were palpable or visible at operation. Type 2 AVMs occurred in seven patients. They were larger and occasionally visible, most commonly in the small intestine, and probably of congenital origin. Symptoms all began before 50 years of age. Type 3 AVMs were punctate angiomas causing gastrointestinal hemorrhage. They occurred in three patients with the classical findings of hereditary hemorrhagic telangiectasia. The diagnosis of most AVMs can be determined preoperatively only by selective angiographic studies."}
{"id": "PMID:1083229", "title": "Aortocoronary artery bypass: present indications and risk factors.", "content": "From 1969 through 1974, a total of 4,522 patients were operated on for coronary artery occlusive disease. This article is an in-depth analysis of a consecutive series of 275 of these patients, operated on during 1974. The mortality was 1.8% and perioperative myocardial infarction occurred in 3.6%. On analysis of risk factors, it was found that 24% of the patients were over the age of 60; 57% had some degree of left ventricular dysfunction; 9% had generalized hypokinesis; 24% were New York Heart Association functional class IV; 13% had left main coronary artery stenosis; and 11% had preinfarction angina. On analysis of the early mortality, the limiting factor was diffuse coronary arteriosclerosis combined with poor ventricular function. This resulted in inadequate or incomplete myocardial revascularization. Since these two risk factors are usually predictable, coronary artery bypass can be recommended not only for patients with intractable angina, but also for patients with impaired left ventricular function associated with angina, and in patients without angina who have a positive stress electrocardiogram.", "contents": "Aortocoronary artery bypass: present indications and risk factors. From 1969 through 1974, a total of 4,522 patients were operated on for coronary artery occlusive disease. This article is an in-depth analysis of a consecutive series of 275 of these patients, operated on during 1974. The mortality was 1.8% and perioperative myocardial infarction occurred in 3.6%. On analysis of risk factors, it was found that 24% of the patients were over the age of 60; 57% had some degree of left ventricular dysfunction; 9% had generalized hypokinesis; 24% were New York Heart Association functional class IV; 13% had left main coronary artery stenosis; and 11% had preinfarction angina. On analysis of the early mortality, the limiting factor was diffuse coronary arteriosclerosis combined with poor ventricular function. This resulted in inadequate or incomplete myocardial revascularization. Since these two risk factors are usually predictable, coronary artery bypass can be recommended not only for patients with intractable angina, but also for patients with impaired left ventricular function associated with angina, and in patients without angina who have a positive stress electrocardiogram."}
{"id": "PMID:1083230", "title": "Left ventricular aneurysmectomy.", "content": "Comparison of preoperative and postoperative studies in 81 patients undergoing left ventricular aneurysmectomy failed to show consistent hemodynamic trends. The most reliable prognostic indicator for survival (84% early, 71% late) was the function of the basilar ventricular segments. In 62 of the 81 patients, there was concomitant aortocoronary bypass grafting. Eighty-eight percent of the surviving patients are essentially free of symptoms. These findings support the continued surgical treatment of ventricular aneurysm in symptomatic patients, and suggest nonoperative treatment for patients who are asymptomatic.", "contents": "Left ventricular aneurysmectomy. Comparison of preoperative and postoperative studies in 81 patients undergoing left ventricular aneurysmectomy failed to show consistent hemodynamic trends. The most reliable prognostic indicator for survival (84% early, 71% late) was the function of the basilar ventricular segments. In 62 of the 81 patients, there was concomitant aortocoronary bypass grafting. Eighty-eight percent of the surviving patients are essentially free of symptoms. These findings support the continued surgical treatment of ventricular aneurysm in symptomatic patients, and suggest nonoperative treatment for patients who are asymptomatic."}
{"id": "PMID:1083231", "title": "Major arterial hemorrhage: a complication of pancreatic pseudocysts and chronic pancreatitis.", "content": "Twelve patients (11 men, one woman), aged 18 to 68 years, had major arterial hemorrhage as a direct complication of pancreatic pseudocysts and chronic pancreatitis. Chronic alcoholism (11 patients) and blunt abdominal trauma (one patient) were the basis for pancreatic disease. Spontaneous hemorrhage occurred in eight patients. Bleeding occurred into the gastrointestinal tract (eight patients), into the peritoneal cavity (four patients), and was intracystic (one patient). Splenic, pancreaticoduodenal, gastroduodenal, and gastroepiploic arteries were sources of hemorrhage. Operative procedures included local control of bleeding (six patients), distal pancreatectomy (three patients), and pancreaticoduodenectomy (one patient). Four patients died of hemorrhagic complications of pancreatic disease, including one not subjected to operation. Extirpation of diseased pancreatic tissue may lessen the morbidity and mortality attributed to this complication of pancreatitis.", "contents": "Major arterial hemorrhage: a complication of pancreatic pseudocysts and chronic pancreatitis. Twelve patients (11 men, one woman), aged 18 to 68 years, had major arterial hemorrhage as a direct complication of pancreatic pseudocysts and chronic pancreatitis. Chronic alcoholism (11 patients) and blunt abdominal trauma (one patient) were the basis for pancreatic disease. Spontaneous hemorrhage occurred in eight patients. Bleeding occurred into the gastrointestinal tract (eight patients), into the peritoneal cavity (four patients), and was intracystic (one patient). Splenic, pancreaticoduodenal, gastroduodenal, and gastroepiploic arteries were sources of hemorrhage. Operative procedures included local control of bleeding (six patients), distal pancreatectomy (three patients), and pancreaticoduodenectomy (one patient). Four patients died of hemorrhagic complications of pancreatic disease, including one not subjected to operation. Extirpation of diseased pancreatic tissue may lessen the morbidity and mortality attributed to this complication of pancreatitis."}
{"id": "PMID:1083232", "title": "[Regeneration of the gastric mucosa under conditions of massive blood loss].", "content": "The autoradiographic method was used for a comparative study of regeneration of the gastric mucosa after wounding the rat with a preliminary loss of blood and without it. The H3-thymidin label index was determined in the portions of the mucosa of the wound edge and far from it. A histological analysis of healing the wounds failed to reveal any difference in terms of this process in rats with a loss of blood and without it. A momentary massive loss of blood did not influence the strain and intracellular regeneration processes in the healing wound of the stomach: the amount of cells synthesizing DNA at the edges of the gastrotonic wound proved to be the same in experiments both with a loss of blood and without it. The authors believe that it may serve as an indirect evidence of a somewhat exaggerated danger of surgery at the height of gastroduodenal bleeding.", "contents": "[Regeneration of the gastric mucosa under conditions of massive blood loss]. The autoradiographic method was used for a comparative study of regeneration of the gastric mucosa after wounding the rat with a preliminary loss of blood and without it. The H3-thymidin label index was determined in the portions of the mucosa of the wound edge and far from it. A histological analysis of healing the wounds failed to reveal any difference in terms of this process in rats with a loss of blood and without it. A momentary massive loss of blood did not influence the strain and intracellular regeneration processes in the healing wound of the stomach: the amount of cells synthesizing DNA at the edges of the gastrotonic wound proved to be the same in experiments both with a loss of blood and without it. The authors believe that it may serve as an indirect evidence of a somewhat exaggerated danger of surgery at the height of gastroduodenal bleeding."}
{"id": "PMID:1083233", "title": "Slow saccades in spinocerebellar degeneration.", "content": "Two patients with spinocerebellar degeneration made abnormally slow horizontal refixations. One patient produced quick phases of nystagmus with identical maximum velocities, suggesting her refixations were abnormal saccades and not voluntary pursuit movements. In response to double target jumps, neither patient showed an obligatory refractory period after each saccade; they responded to every target movement after one reaction time. Their slow refixations were not preprogrammed since they could be modified in flight. To reconcile these observations with normal saccadic behavior, we hypothesized a neural network that made saccades by driving the eyes to an orbital position rather than preprogramming a distance for movement. Computer simulation of this model produced both realistically appearing normal saccades and, when appropriately \"lesioned\" to simulate a loss of saccadic \"burst\" neurons in the pontine reticular formation, slow saccades that could be modified in flight.", "contents": "Slow saccades in spinocerebellar degeneration. Two patients with spinocerebellar degeneration made abnormally slow horizontal refixations. One patient produced quick phases of nystagmus with identical maximum velocities, suggesting her refixations were abnormal saccades and not voluntary pursuit movements. In response to double target jumps, neither patient showed an obligatory refractory period after each saccade; they responded to every target movement after one reaction time. Their slow refixations were not preprogrammed since they could be modified in flight. To reconcile these observations with normal saccadic behavior, we hypothesized a neural network that made saccades by driving the eyes to an orbital position rather than preprogramming a distance for movement. Computer simulation of this model produced both realistically appearing normal saccades and, when appropriately \"lesioned\" to simulate a loss of saccadic \"burst\" neurons in the pontine reticular formation, slow saccades that could be modified in flight."}
{"id": "PMID:1083234", "title": "[Histogenesis of the immune system of the \"nude\" mouse. IV. The ultrastructure of the thymus anlage in 12 and 13 days old embryos (author's transl)].", "content": "The development of the thymus of the heterozygous (nu/+) mouse is indistinguishable from that of \"normal\" mice (Groscurth and Kistler, 1975). However, previous studies have shown that the thymus anlage of the homozygous (nu/nu) mouse differs significantly from that of its nu/+ counterpart. This morphological deviation becomes detectable by light microscopy during the 13th day post conceptionem (p.c.). It was assumed that, at the ultrastructural level, this maldifferentiation could become manifest at an earlier developmental stage. Therefore, the thymus anlage of 12 and 13 day-old nu/nu and ICR embryos was investigated electron microscopically. The \"nude\" mice with the genetic background BALB/c were kept under the same conditions as described previously (Groscurth and Kistler, 1975). Mice of the strain ICR were used as controls. Virgin nu/nu females were mated with nu/nu males and the pregnant animals were sacrificed on the 12th of 13th day p. c. The embryos were fixed in 2.5% glutaraldehyde, postfixed in I% OsO4 and embedded in Epon.", "contents": "[Histogenesis of the immune system of the \"nude\" mouse. IV. The ultrastructure of the thymus anlage in 12 and 13 days old embryos (author's transl)]. The development of the thymus of the heterozygous (nu/+) mouse is indistinguishable from that of \"normal\" mice (Groscurth and Kistler, 1975). However, previous studies have shown that the thymus anlage of the homozygous (nu/nu) mouse differs significantly from that of its nu/+ counterpart. This morphological deviation becomes detectable by light microscopy during the 13th day post conceptionem (p.c.). It was assumed that, at the ultrastructural level, this maldifferentiation could become manifest at an earlier developmental stage. Therefore, the thymus anlage of 12 and 13 day-old nu/nu and ICR embryos was investigated electron microscopically. The \"nude\" mice with the genetic background BALB/c were kept under the same conditions as described previously (Groscurth and Kistler, 1975). Mice of the strain ICR were used as controls. Virgin nu/nu females were mated with nu/nu males and the pregnant animals were sacrificed on the 12th of 13th day p. c. The embryos were fixed in 2.5% glutaraldehyde, postfixed in I% OsO4 and embedded in Epon."}
{"id": "PMID:1083235", "title": "A fine structure study of human thymus.", "content": "A study of the fine structure of human thymus, including normal hyperplastic and with thymoma, is reported in this paper. There is increasing experimental evidence that thymic epithelial cells are a source of hormone. It is, therefore, of interest that we found epithelial cells containing intravesicular cytoplasmic material that reacted with silver methenamine as do mucoproteins. Similar material, possibly extracellular, was seen in close contact with lymphocytes and macrophages in the thymus. The results suggest a possible mechanism for epithelial cell/lymphocyte interaction. Some epithelial cells also showed phagocytic features and all appear to play a structural role. The presence of nuclear pockets was noted within lymphocyte nuclei.", "contents": "A fine structure study of human thymus. A study of the fine structure of human thymus, including normal hyperplastic and with thymoma, is reported in this paper. There is increasing experimental evidence that thymic epithelial cells are a source of hormone. It is, therefore, of interest that we found epithelial cells containing intravesicular cytoplasmic material that reacted with silver methenamine as do mucoproteins. Similar material, possibly extracellular, was seen in close contact with lymphocytes and macrophages in the thymus. The results suggest a possible mechanism for epithelial cell/lymphocyte interaction. Some epithelial cells also showed phagocytic features and all appear to play a structural role. The presence of nuclear pockets was noted within lymphocyte nuclei."}
{"id": "PMID:1083241", "title": "The watershed: a factor in coronary vein graft occlusion.", "content": "In 50 patients with one or more aortocoronary saphenous vein grafts investigated by angiography the patency rate of grafts inserted into arteries with a total proximal occlusion was significantly higher than of those inserted into arteries with a proximal stenosis. The interval between operation and investigation in the two groups was similar. In 10 patients with double grafts, one to an occluded and one to a non-occluded artery, there were 7 with one blocked graft, in each case to the non-occluded artery. In the other 3 both grafts were patent. A watershed was shown at angiography in one patient with a graft inserted into a stenosed artery; the distal run-off appeared good, but reflux of the contrast up the coronary artery into the aorta occurred when injecting into the graft, and vice versa. This watershed may operate to a minor degree in all grafts inserted into non-occluded arteries, and by causing stasis at the anastomosis, could explain the higher incidence of graft occlusion in this group. Ligation of the coronary artery proximal to the anastomosis may therefore be necessary to achieve the highest patency rate.", "contents": "The watershed: a factor in coronary vein graft occlusion. In 50 patients with one or more aortocoronary saphenous vein grafts investigated by angiography the patency rate of grafts inserted into arteries with a total proximal occlusion was significantly higher than of those inserted into arteries with a proximal stenosis. The interval between operation and investigation in the two groups was similar. In 10 patients with double grafts, one to an occluded and one to a non-occluded artery, there were 7 with one blocked graft, in each case to the non-occluded artery. In the other 3 both grafts were patent. A watershed was shown at angiography in one patient with a graft inserted into a stenosed artery; the distal run-off appeared good, but reflux of the contrast up the coronary artery into the aorta occurred when injecting into the graft, and vice versa. This watershed may operate to a minor degree in all grafts inserted into non-occluded arteries, and by causing stasis at the anastomosis, could explain the higher incidence of graft occlusion in this group. Ligation of the coronary artery proximal to the anastomosis may therefore be necessary to achieve the highest patency rate."}
{"id": "PMID:1083242", "title": "Long-term results of arterial counterpulsation in acute severe cardiac failure complicating myocardial infarction.", "content": "Thirty patients were discharged from hospital after treatment with arterial counterpulsation for acute severe heart failure complicating myocardial infarction. Seventeen patients had been in cardiogenic shock--13 with power failure alone, and 4 with a mechanical complication which required corrective surgery. Thirteen patients were in impending or 'preshock'. The follow-up period after infarction averaged 13-1 months. Results were good in 'preshock' patients, and in shocked patients with a surgically correctable mechanical complication. Results were poor in shocked patients with power failure alone. Counterpulsation has an important supportive role in the treatment of complicated myocardial infarction, provided that intervention is prompt and mechanical complications, if present, are corrected early.", "contents": "Long-term results of arterial counterpulsation in acute severe cardiac failure complicating myocardial infarction. Thirty patients were discharged from hospital after treatment with arterial counterpulsation for acute severe heart failure complicating myocardial infarction. Seventeen patients had been in cardiogenic shock--13 with power failure alone, and 4 with a mechanical complication which required corrective surgery. Thirteen patients were in impending or 'preshock'. The follow-up period after infarction averaged 13-1 months. Results were good in 'preshock' patients, and in shocked patients with a surgically correctable mechanical complication. Results were poor in shocked patients with power failure alone. Counterpulsation has an important supportive role in the treatment of complicated myocardial infarction, provided that intervention is prompt and mechanical complications, if present, are corrected early."}
{"id": "PMID:1083237", "title": "Esophageal varices in Felty's syndrome: A case report and review of the literature.", "content": "A case of upper gastrointestinal tract hemorrhage secondary to esophageal varices in a patient with Felty's syndrome prompted a review of the pathogenesis and treatment of this condition. Six previously reported cases of this association were found. The clinical picture is that of long-standing rheumatoid arthritis with severe articular and extraarticular manifestations including splenomegaly, depression of the blood elements, mild liver function abnormalities, portal hypertension without cirrhosis or portal vein obstruction, an elevated splenic blood flow, and a reduction in portal hypertension by simple splenectomy. The presence of portal hypertension with varices may be another indication of splenectomy in patients with Felty's syndrome.", "contents": "Esophageal varices in Felty's syndrome: A case report and review of the literature. A case of upper gastrointestinal tract hemorrhage secondary to esophageal varices in a patient with Felty's syndrome prompted a review of the pathogenesis and treatment of this condition. Six previously reported cases of this association were found. The clinical picture is that of long-standing rheumatoid arthritis with severe articular and extraarticular manifestations including splenomegaly, depression of the blood elements, mild liver function abnormalities, portal hypertension without cirrhosis or portal vein obstruction, an elevated splenic blood flow, and a reduction in portal hypertension by simple splenectomy. The presence of portal hypertension with varices may be another indication of splenectomy in patients with Felty's syndrome."}
{"id": "PMID:1083238", "title": "Comprehensive study of humoral and cellular immune abnormalities in 26 patients with systemic amyloidosis.", "content": "Serum immunoglobulin abnromalities in nonfamilial and systemic primary amyloidosis are distinct from those seen in multiple myeloma and more closely resemble those seen in benign monoclonal gammopathy. Surface lymphocyte characteristics also differentiate amyloid patients with or without monoclonal immunoglobulins from those with malignant B-cell dyscrasias. In vitro lymphocyte transformation with phytohemagglutinin and pokeweed mitogen is normal in amyloidosis, but T-cell function is significantly depressed in both primary and secondary amyloid disease as measured by concanavalin A responses.", "contents": "Comprehensive study of humoral and cellular immune abnormalities in 26 patients with systemic amyloidosis. Serum immunoglobulin abnromalities in nonfamilial and systemic primary amyloidosis are distinct from those seen in multiple myeloma and more closely resemble those seen in benign monoclonal gammopathy. Surface lymphocyte characteristics also differentiate amyloid patients with or without monoclonal immunoglobulins from those with malignant B-cell dyscrasias. In vitro lymphocyte transformation with phytohemagglutinin and pokeweed mitogen is normal in amyloidosis, but T-cell function is significantly depressed in both primary and secondary amyloid disease as measured by concanavalin A responses."}
{"id": "PMID:1083239", "title": "Antibody-mediated lymphocytotoxicity in rheumatoid arthritis and systemic lupus erythematosus.", "content": "Lymphocyte-dependent antibody cytotoxicity (LDAC) was studied using peripheral blood and in some instances synovial fluid cells from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). No difference from normal controls was observed with peripheral blood lymphocytes from either RA or SLE. Marked decrement in LDAC effector cell activity was present particularly with RA synovial fluid cells. Sera from patients with RA or SLE as well as RA synovial fluids markedly inhibited LDAC.", "contents": "Antibody-mediated lymphocytotoxicity in rheumatoid arthritis and systemic lupus erythematosus. Lymphocyte-dependent antibody cytotoxicity (LDAC) was studied using peripheral blood and in some instances synovial fluid cells from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). No difference from normal controls was observed with peripheral blood lymphocytes from either RA or SLE. Marked decrement in LDAC effector cell activity was present particularly with RA synovial fluid cells. Sera from patients with RA or SLE as well as RA synovial fluids markedly inhibited LDAC."}
{"id": "PMID:1083245", "title": "The effect of methotrexate on spontaneous mammary adenocarcinomata in female C3H mice.", "content": "The effect of methotrexate on solid rodent tumours has been investigated using the spontaneous mammary adenocarcinoma of the female C3H/Bts mouse. As these tumours exhibit a wide range of volume doubling times, calculations of tumour response to methotrexate must be related to doubling time. When methotrexate is injected into the tumour a dose-dependent tumour response is obtained. Systemic citrovorum \"rescue\" prevents methotrexate lethalities but reduces tumour response by a factor of 2. Methotrexate-treated tumours have an increased volume doubling time post treatment.", "contents": "The effect of methotrexate on spontaneous mammary adenocarcinomata in female C3H mice. The effect of methotrexate on solid rodent tumours has been investigated using the spontaneous mammary adenocarcinoma of the female C3H/Bts mouse. As these tumours exhibit a wide range of volume doubling times, calculations of tumour response to methotrexate must be related to doubling time. When methotrexate is injected into the tumour a dose-dependent tumour response is obtained. Systemic citrovorum \"rescue\" prevents methotrexate lethalities but reduces tumour response by a factor of 2. Methotrexate-treated tumours have an increased volume doubling time post treatment."}
{"id": "PMID:1083246", "title": "Lysosomal acid hydrolases in human lymphocyte subpopulations.", "content": "Acid phosphatase and beta-glucuronidase activities have been determined cytochemically in T, B and null lymphocytes as part of an effort to charaterize the human haemopoietic stem cell. A combination of weak acid phosphatase activity and strong beta glucuronidase staining, in the form of a single large granule, has been shown to be specific for T cells. On the basis of this approach alone, non-T cells could not be further subclassified. Further cytochemical evaluation is being explored.", "contents": "Lysosomal acid hydrolases in human lymphocyte subpopulations. Acid phosphatase and beta-glucuronidase activities have been determined cytochemically in T, B and null lymphocytes as part of an effort to charaterize the human haemopoietic stem cell. A combination of weak acid phosphatase activity and strong beta glucuronidase staining, in the form of a single large granule, has been shown to be specific for T cells. On the basis of this approach alone, non-T cells could not be further subclassified. Further cytochemical evaluation is being explored."}
{"id": "PMID:1083247", "title": "Biosynthesis of 3'-deoxyadenosine by Cordyceps militaris. Mechanism of reduction.", "content": "The biosynthesis of 3'-deoxyadenosine (cordycepin) by Cordyceps militaris has been investigated using [U-14C]adenosine and [3-3H]ribose. Crystallization of the resulting radioactive 3'-deoxyadenosine to a constant specific activity showed incorporation of both labeled compounds. A control showed that the 3H:14C ratio of the AMP isolated from the RNA was the same as the 3H:14C ratio in the 3'-deoxyadenosine. The 14C ratio in the adenine: ribose of the [U-14C]adenosine added to the 3'-deoxyadenosine producing cultures of C. militaris and of the isolated 3'-deoxyadenosine was the same, e.g. 50:50. These data provide strong evidence that adenosine in converted to 3'-deoxyadenosine without hydrolysis of the N-riboside bond. Degradation of the 3-deoxyribose from 3'-deoxyadenosine showed that the 3H was retained on carbon-3. These results suggest that the formation of 3'-deoxyadenosine may proceed by a reductive mechanism similar to that for the formation of 2'-deoxynucleotides.", "contents": "Biosynthesis of 3'-deoxyadenosine by Cordyceps militaris. Mechanism of reduction. The biosynthesis of 3'-deoxyadenosine (cordycepin) by Cordyceps militaris has been investigated using [U-14C]adenosine and [3-3H]ribose. Crystallization of the resulting radioactive 3'-deoxyadenosine to a constant specific activity showed incorporation of both labeled compounds. A control showed that the 3H:14C ratio of the AMP isolated from the RNA was the same as the 3H:14C ratio in the 3'-deoxyadenosine. The 14C ratio in the adenine: ribose of the [U-14C]adenosine added to the 3'-deoxyadenosine producing cultures of C. militaris and of the isolated 3'-deoxyadenosine was the same, e.g. 50:50. These data provide strong evidence that adenosine in converted to 3'-deoxyadenosine without hydrolysis of the N-riboside bond. Degradation of the 3-deoxyribose from 3'-deoxyadenosine showed that the 3H was retained on carbon-3. These results suggest that the formation of 3'-deoxyadenosine may proceed by a reductive mechanism similar to that for the formation of 2'-deoxynucleotides."}
{"id": "PMID:1083248", "title": "Circular dichroism of rhodopsins and porphyropsins.", "content": "Circular dichroism and absorption spectra were determined for digitonin extracts of three rhodopsins: cattle, grass frog, and pigeon; and three porphyropsins: channel catfish, bluegill sunfish, and redear sunfish. A comparison of these spectra shows the following: (1) Porphyropsins, like rhodopsins, exhibit two positive CD peaks in the spectral region 320--700 nm: an alpha peak at about 520 nm and a small beta peak at about 355 nm. These peaks substantially diminish upon bleaching. (2) In the CD spectra the alpha peaks of the porphyropsins are larger than the alpha peaks of the rhodopsins, while the beta peaks are smaller than those of the rhodopsins. This is just the opposite of the corresponding relationship between the peaks in the absorption spectra. (3) The maxima of these peaks in the CD spectra of rhodopsins and porphyropsins are consistently blueshifted from corresponding maxima in absorption spectra. (4) Some of the visual pigments show additional positive CD peaks in the spectral region 250--320 nm. In all the visual pigments studied, the CD spectra in this region decrease on bleaching. No reciprocal relationship is observed between any of the CD bands in the visible and near ultraviolet region of the spectrum.", "contents": "Circular dichroism of rhodopsins and porphyropsins. Circular dichroism and absorption spectra were determined for digitonin extracts of three rhodopsins: cattle, grass frog, and pigeon; and three porphyropsins: channel catfish, bluegill sunfish, and redear sunfish. A comparison of these spectra shows the following: (1) Porphyropsins, like rhodopsins, exhibit two positive CD peaks in the spectral region 320--700 nm: an alpha peak at about 520 nm and a small beta peak at about 355 nm. These peaks substantially diminish upon bleaching. (2) In the CD spectra the alpha peaks of the porphyropsins are larger than the alpha peaks of the rhodopsins, while the beta peaks are smaller than those of the rhodopsins. This is just the opposite of the corresponding relationship between the peaks in the absorption spectra. (3) The maxima of these peaks in the CD spectra of rhodopsins and porphyropsins are consistently blueshifted from corresponding maxima in absorption spectra. (4) Some of the visual pigments show additional positive CD peaks in the spectral region 250--320 nm. In all the visual pigments studied, the CD spectra in this region decrease on bleaching. No reciprocal relationship is observed between any of the CD bands in the visible and near ultraviolet region of the spectrum."}
{"id": "PMID:1083249", "title": "The rate of rhodopsin phosphorylation in isolated rentinas of frog and cattle.", "content": "Phosphorylation of rhodopsin has been measured in isolated retinas incubated with 32P-phosphate under physiological conditions. The half-time of the light-induced phosphorylation was found to be approximately 2 min with frog retinas at 21 degrees C, and in the order of 1--2 min with cattle retinas at 36 degrees C. It is suggested by this slow rate that the phosphorylation reaction is not directly involved in the chain of events which lead from absorption of a photon to excitation of the photoreceptor cells but may perhaps have a regulatory function in controlling light/dark adaptation.", "contents": "The rate of rhodopsin phosphorylation in isolated rentinas of frog and cattle. Phosphorylation of rhodopsin has been measured in isolated retinas incubated with 32P-phosphate under physiological conditions. The half-time of the light-induced phosphorylation was found to be approximately 2 min with frog retinas at 21 degrees C, and in the order of 1--2 min with cattle retinas at 36 degrees C. It is suggested by this slow rate that the phosphorylation reaction is not directly involved in the chain of events which lead from absorption of a photon to excitation of the photoreceptor cells but may perhaps have a regulatory function in controlling light/dark adaptation."}
{"id": "PMID:1083250", "title": "Cytidine-5'-monophosphate-N-acetylneuraminic acid. Asialoglycoprotein sialic acid transferase activity in liver and serum of patients with juvenile hepatic cirrhosis and alpha-1-antitrypsin deficiency.", "content": "The molecular basis for the accumulation of a substance which displays the immunological reactivity of alpha-1-antitrypsin within vesicles of liver parenchymal cells of individuals with hepatic cirrhosis and serum alpha-1-antitrypsin deficiency remains unclear. We recently reported that serum from a patient with alpha-1-antitrypsin deficiency and hepatic cirrhosis was substantially deficient in sialyltransferease (EC 2.4.99.1) an enzyme which transfers sialic acid from cytidine 5'-monophosphate-N-acetylneuraminic acid to a variety of asialoglycoprotein acceptors. In the present report we have extended these studies to include serum from five additional patients with alpha-1-antitrypsin deficiency and juvenile hepatic cirrhosis as well as a liver specimen obtained at autopsy of one of these patients. We find the sialytransferase activity in serum from six patients with alpha-1-antitrypsin deficiency and hepatic cirrhosis to be 50% of healthy pediatric control values and 30% of pediatric patients with liver disease. However, serum from family members homozygous for alpha-1-antitrypsin deficiency but without hepatic cirrhosis, and serum from patients with a variety of other kinds of liver disease, failed to exhibit the marked sialytransferase deficiency. Similar assays carried out on a homogenate of a liver sample from one patient with alpha-1-antitrypsin deficiency and hepatic cirrhosis indicated that the deficiency of sialyltransferase activity was not demonstrable in liver. Furthermore, a comparative kinetic analysis of serum and liver sialytransferase in normal and afflicted individuals failed to detect differences in substrate affinities which might account for a decrease in functional sialyltransferase capacity in individuals with alpha-1-antitrypsin deficiency and hepatic cirrhosis. These observations suggest that the serum sialyltransferase deficiency in such patients probably arises after chronic and extensive liver disease involving hepatic accumulation of alpha-1-antitrypsin rather than the enzyme deficiency being the primary cause of the hepatic cirrhosis and alpha-1-antitrypsin deficiency.", "contents": "Cytidine-5'-monophosphate-N-acetylneuraminic acid. Asialoglycoprotein sialic acid transferase activity in liver and serum of patients with juvenile hepatic cirrhosis and alpha-1-antitrypsin deficiency. The molecular basis for the accumulation of a substance which displays the immunological reactivity of alpha-1-antitrypsin within vesicles of liver parenchymal cells of individuals with hepatic cirrhosis and serum alpha-1-antitrypsin deficiency remains unclear. We recently reported that serum from a patient with alpha-1-antitrypsin deficiency and hepatic cirrhosis was substantially deficient in sialyltransferease (EC 2.4.99.1) an enzyme which transfers sialic acid from cytidine 5'-monophosphate-N-acetylneuraminic acid to a variety of asialoglycoprotein acceptors. In the present report we have extended these studies to include serum from five additional patients with alpha-1-antitrypsin deficiency and juvenile hepatic cirrhosis as well as a liver specimen obtained at autopsy of one of these patients. We find the sialytransferase activity in serum from six patients with alpha-1-antitrypsin deficiency and hepatic cirrhosis to be 50% of healthy pediatric control values and 30% of pediatric patients with liver disease. However, serum from family members homozygous for alpha-1-antitrypsin deficiency but without hepatic cirrhosis, and serum from patients with a variety of other kinds of liver disease, failed to exhibit the marked sialytransferase deficiency. Similar assays carried out on a homogenate of a liver sample from one patient with alpha-1-antitrypsin deficiency and hepatic cirrhosis indicated that the deficiency of sialyltransferase activity was not demonstrable in liver. Furthermore, a comparative kinetic analysis of serum and liver sialytransferase in normal and afflicted individuals failed to detect differences in substrate affinities which might account for a decrease in functional sialyltransferase capacity in individuals with alpha-1-antitrypsin deficiency and hepatic cirrhosis. These observations suggest that the serum sialyltransferase deficiency in such patients probably arises after chronic and extensive liver disease involving hepatic accumulation of alpha-1-antitrypsin rather than the enzyme deficiency being the primary cause of the hepatic cirrhosis and alpha-1-antitrypsin deficiency."}
{"id": "PMID:1083251", "title": "Inhibition of urokinase by complex formation with human alpha1-antitrypsin.", "content": "Human alpha1-antitrypsin was prepared from fresh human plasma by (NH4)-SO4-precipitation, gel filtration, affinity chromatography on concanavalin A, ion exchange chromatography and isotachophoresis. Human urokinase (EC 3.4.99.26) (plasminogen activator from urine) with M, 46 000 and 36 000 was further purified from Urokinase Leo reagent preparation by gel filtration on Sephadex G-100 Superfine. The hydrolytic activity of urokinase on acetyl-glycyl-L-lysine methyl ester acetate (Ac-Gly-Lys-OMeAc) was inhibited in a strong time-dependent manner by alpha1-antitrypsin. Complex formation between enzyme and inhibitor could be demonstrated in crossed immunoelectrophoresis against anti-alpha1-antitrypsin and anti-urokinase serum as well as by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The latter method revealed the formation of 1:1 and 2:1 molar enzyme-inhibitor complexes.", "contents": "Inhibition of urokinase by complex formation with human alpha1-antitrypsin. Human alpha1-antitrypsin was prepared from fresh human plasma by (NH4)-SO4-precipitation, gel filtration, affinity chromatography on concanavalin A, ion exchange chromatography and isotachophoresis. Human urokinase (EC 3.4.99.26) (plasminogen activator from urine) with M, 46 000 and 36 000 was further purified from Urokinase Leo reagent preparation by gel filtration on Sephadex G-100 Superfine. The hydrolytic activity of urokinase on acetyl-glycyl-L-lysine methyl ester acetate (Ac-Gly-Lys-OMeAc) was inhibited in a strong time-dependent manner by alpha1-antitrypsin. Complex formation between enzyme and inhibitor could be demonstrated in crossed immunoelectrophoresis against anti-alpha1-antitrypsin and anti-urokinase serum as well as by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The latter method revealed the formation of 1:1 and 2:1 molar enzyme-inhibitor complexes."}
{"id": "PMID:1083252", "title": "The type, amount, location, and energy transfer properties of the carotenoid in reaction centers from Rhodopseudomonas sphaeroides.", "content": "Analysis of photosynthetic reaction centers from Rhodopseudomonas sphaeroides strains 2.4.1 and Ga shows that each contains approx. 1 mol of a specific carotenoid per mol of reaction center. In strain 2.4.1. the carotenoid is spheroidene (1-methoxy-3,4-didehydro-1,2,7',8',-tetrahydro-psi,psi-carotene); in strain Ga, it is chloroxanthin (1-hydroxy-1, 2, 7', 8'-tetrahydro-psi,psi-carotene). The carotenoid is bound to the same pair of proteins as are the bacteriochlorophylls and bacteriopheophytins of the reaction center. This binding induces strong circular dichroism in the absorption bands of the carotenoid. The carotenoid is close enough to the other pigments of the reaction center so that light energy transfers efficiently from the carotenoid to the bacteriochlorophyll, sensitizing bacteriochlorophyll fluorescence. The fluorescence polarization spectrum of the reaction centers shows that the transition vectors for the visible absorption bands of the carotenoid lie approximately parallel to the 600 nm (Qx) transition of the bacteriochlorophyll complex.", "contents": "The type, amount, location, and energy transfer properties of the carotenoid in reaction centers from Rhodopseudomonas sphaeroides. Analysis of photosynthetic reaction centers from Rhodopseudomonas sphaeroides strains 2.4.1 and Ga shows that each contains approx. 1 mol of a specific carotenoid per mol of reaction center. In strain 2.4.1. the carotenoid is spheroidene (1-methoxy-3,4-didehydro-1,2,7',8',-tetrahydro-psi,psi-carotene); in strain Ga, it is chloroxanthin (1-hydroxy-1, 2, 7', 8'-tetrahydro-psi,psi-carotene). The carotenoid is bound to the same pair of proteins as are the bacteriochlorophylls and bacteriopheophytins of the reaction center. This binding induces strong circular dichroism in the absorption bands of the carotenoid. The carotenoid is close enough to the other pigments of the reaction center so that light energy transfers efficiently from the carotenoid to the bacteriochlorophyll, sensitizing bacteriochlorophyll fluorescence. The fluorescence polarization spectrum of the reaction centers shows that the transition vectors for the visible absorption bands of the carotenoid lie approximately parallel to the 600 nm (Qx) transition of the bacteriochlorophyll complex."}
{"id": "PMID:1083253", "title": "Investigation of peptide-nucleotide interactions using template chromatography. The specific recognition of peptide sequences by oligodeoxy adenylic acids.", "content": "Polyvinylalcohol has been substituted with oligodeoxyadenylic acid and the resulting polyanion polyvinyl (pA)n irreversibly attached to DEAE-cellulose. Peptide-oligonucleotide interactions have been studied using a column chromatographic technique with the polyvinyl (pA)n-DEAE-cellulose as a stationary phase.", "contents": "Investigation of peptide-nucleotide interactions using template chromatography. The specific recognition of peptide sequences by oligodeoxy adenylic acids. Polyvinylalcohol has been substituted with oligodeoxyadenylic acid and the resulting polyanion polyvinyl (pA)n irreversibly attached to DEAE-cellulose. Peptide-oligonucleotide interactions have been studied using a column chromatographic technique with the polyvinyl (pA)n-DEAE-cellulose as a stationary phase."}
{"id": "PMID:1083254", "title": "Antinuclear factor-positive mental depression: a single disease entity?", "content": "Antinuclear factor (ANF) was present in the serum of about 30% of 53 patients newly admitted to the psychiatric hospital because of mental depression. Clinically, ANF-positive depression closely resembles manic-depressive psychosis but tends to be more resistant to treatment. It is suggested that ANF-positive depression may be a quite distinctive disease.", "contents": "Antinuclear factor-positive mental depression: a single disease entity? Antinuclear factor (ANF) was present in the serum of about 30% of 53 patients newly admitted to the psychiatric hospital because of mental depression. Clinically, ANF-positive depression closely resembles manic-depressive psychosis but tends to be more resistant to treatment. It is suggested that ANF-positive depression may be a quite distinctive disease."}
{"id": "PMID:1083256", "title": "Electrophoretic mobility of blood lymphocytes in cancer patients.", "content": "Determination of electrophoretic mobility of lymphocytes in 70 cancer patients shows a significant decrease of the fast moving cells. It seems to be a relation between the percentage or absolute number of the rapid lymphocytes and dissemination and prognosis of the disease. This decrease is in agreement with the variations of the T lymphocyte population described in cancer patients with other methods. On the contrary, although there is reduction of total blood lymphocytes, the number of slow moving cells is not reduced. The changes observed in mean migration speed of fast and slow migrating lymphocytes could indicate more selective alterations in some cell subpopulations.", "contents": "Electrophoretic mobility of blood lymphocytes in cancer patients. Determination of electrophoretic mobility of lymphocytes in 70 cancer patients shows a significant decrease of the fast moving cells. It seems to be a relation between the percentage or absolute number of the rapid lymphocytes and dissemination and prognosis of the disease. This decrease is in agreement with the variations of the T lymphocyte population described in cancer patients with other methods. On the contrary, although there is reduction of total blood lymphocytes, the number of slow moving cells is not reduced. The changes observed in mean migration speed of fast and slow migrating lymphocytes could indicate more selective alterations in some cell subpopulations."}
{"id": "PMID:1083257", "title": "Formation of mouse red cell rosettes by \"hairy\" cells.", "content": "Rosette formation with mouse red cells was observed in the \"hairy\" cells of four cases of leukaemic reticuloendotheliosis. A similar observation was made on B-lymphocytes from chronic lymphoid leukaemia but the monocytes in monocytoid leukaemia did not form rosettes. This finding provides new evidence for the B-cell nature of the \"hairy\" cell.", "contents": "Formation of mouse red cell rosettes by \"hairy\" cells. Rosette formation with mouse red cells was observed in the \"hairy\" cells of four cases of leukaemic reticuloendotheliosis. A similar observation was made on B-lymphocytes from chronic lymphoid leukaemia but the monocytes in monocytoid leukaemia did not form rosettes. This finding provides new evidence for the B-cell nature of the \"hairy\" cell."}
{"id": "PMID:1083258", "title": "Ultrastructural abnormalities of the chondrocytes of the growth cartilage of a type of poly-epiphyseal dysplasia with a probable autosomal recessive transmission.", "content": "In two cases of polyepiphyseal dysplasia with a probable autosomal recessive transmission, ultrastructural abnormalities were found in the chondrocytes of the tibial epiphyseal growth plate. The largest part of the chondrocytes is occupied by many large vacuoles. They are bounded by a single membrane and have an electron-lucent background in which electron-opaque granules and delicate spiral filaments are visible. The ultrastructural abnormalities suggest a storage disorder probably limited to the chondrocytes.", "contents": "Ultrastructural abnormalities of the chondrocytes of the growth cartilage of a type of poly-epiphyseal dysplasia with a probable autosomal recessive transmission. In two cases of polyepiphyseal dysplasia with a probable autosomal recessive transmission, ultrastructural abnormalities were found in the chondrocytes of the tibial epiphyseal growth plate. The largest part of the chondrocytes is occupied by many large vacuoles. They are bounded by a single membrane and have an electron-lucent background in which electron-opaque granules and delicate spiral filaments are visible. The ultrastructural abnormalities suggest a storage disorder probably limited to the chondrocytes."}
{"id": "PMID:1083259", "title": "[Effect of thymocyte autotransplantation on postradiation regeneration of hematopoiesis].", "content": "Experiments were conducted on Wistar rats irradiated in a dose of 400 R; a study was made of the effect of thymocyte autotransplantation (4-10(8)--8-10(8)) on the restoration of cell number of the bone marrow and the spleen. There was revealed a significant stimulating effect of autotransplantation of thymocytes on the erythroid and granulocytic series in the bone marrow and on the lymphoid one in the spleen during the restoration (the 5 th-- 7 th days) after the irradiation. Irradiation of the thymocyte suspension with gamma-rays in a dose of 10 000 R failed to prevent the effect of autotransplantation on the bone marrow, but decreased its stimulating effect. A conclusion was drawn on the interaction of the administered thymocytes with the stem hemopoietic cell.", "contents": "[Effect of thymocyte autotransplantation on postradiation regeneration of hematopoiesis]. Experiments were conducted on Wistar rats irradiated in a dose of 400 R; a study was made of the effect of thymocyte autotransplantation (4-10(8)--8-10(8)) on the restoration of cell number of the bone marrow and the spleen. There was revealed a significant stimulating effect of autotransplantation of thymocytes on the erythroid and granulocytic series in the bone marrow and on the lymphoid one in the spleen during the restoration (the 5 th-- 7 th days) after the irradiation. Irradiation of the thymocyte suspension with gamma-rays in a dose of 10 000 R failed to prevent the effect of autotransplantation on the bone marrow, but decreased its stimulating effect. A conclusion was drawn on the interaction of the administered thymocytes with the stem hemopoietic cell."}
{"id": "PMID:1083260", "title": "[Hyperpolarization of frog skin exposed to furosemide].", "content": "When applied only to the outer surface of the isolated frog skin furosemide caused a prompt, dose-related (0.1--1.0 mg/ml) and fully reversible increase in potential difference; it did not change the short-circuit current, however. Potential differences were not altered by furosemide in the Ringer-sulphate solution. Furosemide-induced increase of potential differences could be reversed by nystatine which increased the ionic permeability. These results suggest that furosemide could decrease the passive entrance of chlorides through the barrier of the apical cell membrane of the frog skin.", "contents": "[Hyperpolarization of frog skin exposed to furosemide]. When applied only to the outer surface of the isolated frog skin furosemide caused a prompt, dose-related (0.1--1.0 mg/ml) and fully reversible increase in potential difference; it did not change the short-circuit current, however. Potential differences were not altered by furosemide in the Ringer-sulphate solution. Furosemide-induced increase of potential differences could be reversed by nystatine which increased the ionic permeability. These results suggest that furosemide could decrease the passive entrance of chlorides through the barrier of the apical cell membrane of the frog skin."}
{"id": "PMID:1083261", "title": "[Aggregate-hemagglutination test for anti-erythrocyte antibodies].", "content": "A new test for detection of antierythrocyte antibodies is described; one of its variants is three orders of magnitude more sensitive than the classic Coombs test. The proposed procedures are based on the agglutination of the red blood cells following addition of test-erythrocytes coated with the aggregated proteins of an antiglobulin serum. Results of application of the technique for diagnosis of the autoimmune hemolytic anemia showed its high specificity and the absence of prozone phenomenon.", "contents": "[Aggregate-hemagglutination test for anti-erythrocyte antibodies]. A new test for detection of antierythrocyte antibodies is described; one of its variants is three orders of magnitude more sensitive than the classic Coombs test. The proposed procedures are based on the agglutination of the red blood cells following addition of test-erythrocytes coated with the aggregated proteins of an antiglobulin serum. Results of application of the technique for diagnosis of the autoimmune hemolytic anemia showed its high specificity and the absence of prozone phenomenon."}
{"id": "PMID:1083262", "title": "[Cytotoxic effect of lymphotoxin on a culture of macrophages].", "content": "Results of study of the cytotoxic effect of the lymphotoxin on the monolayer culture of the peritoneal macrophages used as target cells are presented. The lymphotoxin was obtained following an 18-hour incubation of the lymph node cells of guinea pigs with delayed hypersensitivity to tuberculoproteins, in the presence of the specific antigen (tuberculin). It was shown that only on the 5th--6th day of cultivation did the supernatants destroy more than half of the macrophages in 70% of the experiments. The death of the macrophages during the later periods of cultivation, in comparing with the L-cells, suggests a different resistance of these target-cells to the cytotoxic effect of the lymphotoxin.", "contents": "[Cytotoxic effect of lymphotoxin on a culture of macrophages]. Results of study of the cytotoxic effect of the lymphotoxin on the monolayer culture of the peritoneal macrophages used as target cells are presented. The lymphotoxin was obtained following an 18-hour incubation of the lymph node cells of guinea pigs with delayed hypersensitivity to tuberculoproteins, in the presence of the specific antigen (tuberculin). It was shown that only on the 5th--6th day of cultivation did the supernatants destroy more than half of the macrophages in 70% of the experiments. The death of the macrophages during the later periods of cultivation, in comparing with the L-cells, suggests a different resistance of these target-cells to the cytotoxic effect of the lymphotoxin."}
{"id": "PMID:1083263", "title": "Granulopoiesis in severe congenital neutropenia.", "content": "The pathogenesis of the granulopoietic failure in three children with severe congenital neutropenia was studied. Mature neutrophils were absent from both peripheral blood and bone marrow. Assay of bone marrow granulocyte colony-forming cells (CFU-C) in a methylcellulose tissue culture system using colony-stimulating activity (CSA) from peripheral blood leukocytes demonstrated normal or increased concentrations of CFU-C compared to those from marrows of 60 age-matched controls. Colonies were of normal size and by light microscopy appeared to contain granulocytes in all stages of maturation including the mature polymorphonuclear neutrophil. CFU-C from peripheral blood of two patients were normal. Production and activity of CSA from the patients' peripheral blood leukocytes and urinary CSA excretion were normal. No serum inhibitors against CFU-C or CSA could be demonstrated using both control and autologous marrow. The defect did not appear to be due to a lack of granulocytic stem cells, a reduction of humoral stimulators of granulopoiesis, nor the presence of an inhibitor as measured by these techniques.", "contents": "Granulopoiesis in severe congenital neutropenia. The pathogenesis of the granulopoietic failure in three children with severe congenital neutropenia was studied. Mature neutrophils were absent from both peripheral blood and bone marrow. Assay of bone marrow granulocyte colony-forming cells (CFU-C) in a methylcellulose tissue culture system using colony-stimulating activity (CSA) from peripheral blood leukocytes demonstrated normal or increased concentrations of CFU-C compared to those from marrows of 60 age-matched controls. Colonies were of normal size and by light microscopy appeared to contain granulocytes in all stages of maturation including the mature polymorphonuclear neutrophil. CFU-C from peripheral blood of two patients were normal. Production and activity of CSA from the patients' peripheral blood leukocytes and urinary CSA excretion were normal. No serum inhibitors against CFU-C or CSA could be demonstrated using both control and autologous marrow. The defect did not appear to be due to a lack of granulocytic stem cells, a reduction of humoral stimulators of granulopoiesis, nor the presence of an inhibitor as measured by these techniques."}
{"id": "PMID:1083264", "title": "Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin.", "content": "B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.", "contents": "Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin. B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin."}
{"id": "PMID:1083265", "title": "Colony-stimulating factor in patients with chronic neutropenia.", "content": "Urinary and serum colony-stimulating factor (CSF) levels were measured in 11 patients with chronic idiopathic neutropenia without infections and in 10 normal individuals. Urinary CSF output was determined using mouse marrow target cells, and serum CSF activity was assayed with human marrow target cells by the double agar layer technique. Using these methods, there was no significant difference between CSF levels of neutropenic and normal subjects. These data indicate that CSF levels are not inversely related to the blood neutrophil count in chronic idiopathic neutropenia and suggest that CSF is not a hormone regulating the blood neutrophil count in a manner analogous to the erythropoietin regulation of circulating erythrocyte levels.", "contents": "Colony-stimulating factor in patients with chronic neutropenia. Urinary and serum colony-stimulating factor (CSF) levels were measured in 11 patients with chronic idiopathic neutropenia without infections and in 10 normal individuals. Urinary CSF output was determined using mouse marrow target cells, and serum CSF activity was assayed with human marrow target cells by the double agar layer technique. Using these methods, there was no significant difference between CSF levels of neutropenic and normal subjects. These data indicate that CSF levels are not inversely related to the blood neutrophil count in chronic idiopathic neutropenia and suggest that CSF is not a hormone regulating the blood neutrophil count in a manner analogous to the erythropoietin regulation of circulating erythrocyte levels."}
{"id": "PMID:1083266", "title": "[Fc receptors and surface immunoglobulins in cells of hairy cell leukemia].", "content": "Using 125I-labelled aggregated IgG in a quantitative assay a strong expression of Fc-receptors was found on the leukemic cells of a patient with hairy cell leukemia. The Fc-receptor activity on these cells was much higher than that on monocytes and B-lymphocytes from normal blood. Surface immunoglobulins were detected by radioautography using radioactively labelled (Fab')2-fragments of monospecific antibodies directed against immunoglobulin heavy chains. Prior to radioautography the cells were stained for the tartrate resistant acid phosphatase. It was found that all cells containing this enzyme bore sigma-chains on their surface. On more than 90% of these cells a simultaneous expression of mu-chains was detected. gamma-Chains could only be demonstrated on cells which were negative for the tartrate resistant acid phosphatase; part of these cells, however, were hairy cells by morphological criteria.", "contents": "[Fc receptors and surface immunoglobulins in cells of hairy cell leukemia]. Using 125I-labelled aggregated IgG in a quantitative assay a strong expression of Fc-receptors was found on the leukemic cells of a patient with hairy cell leukemia. The Fc-receptor activity on these cells was much higher than that on monocytes and B-lymphocytes from normal blood. Surface immunoglobulins were detected by radioautography using radioactively labelled (Fab')2-fragments of monospecific antibodies directed against immunoglobulin heavy chains. Prior to radioautography the cells were stained for the tartrate resistant acid phosphatase. It was found that all cells containing this enzyme bore sigma-chains on their surface. On more than 90% of these cells a simultaneous expression of mu-chains was detected. gamma-Chains could only be demonstrated on cells which were negative for the tartrate resistant acid phosphatase; part of these cells, however, were hairy cells by morphological criteria."}
{"id": "PMID:1083267", "title": "[Anti-immunoglobulins in multiple myeloma and benign monoclonal hyperglobulinemia].", "content": "Sera from 50 healthy adults, from 21 patients with multiple myeloma (MM) and from 11 patients with benign monoclonal hyperglobulinaemia (BMH) and from 28 patients with sarcoidosis were examined for the presence of anti-IgG-activity by passive haemagglutination technique. 62% of healthy adults (titre less than 2) and all of the sera from patients with MM and BMH (titres 32-512) as well as 42% of the sera from sarcoidosis patients were found to be anti-IgG positive. The anti-IgG positive sera showed also anti-(Fab)2-activity (with the exeption of 2 sera from sarcoidosis patients). No significant differences could be found between anti-Ig activity in sera from MM patients with BMH. There was also seen no correlation of anti-Ig with the clinical course of the disease. After column chromatography we could detect the partially simultaneous presence of anti-Ig with anti-Fc-specificity (MW ca. 900,000) and with (Fab)2-specificity (MW 150,000 or less than 90,000). Antibody dependent cytotoxicity (using melanoma cell lines as targets) was significantly inhibited by the isolated anti-Ig-fractions with low molecular sizes (MW less than 90,000). From these results it seems possible that anti-immunoglobulins may play a role in the clinical course of the disease.", "contents": "[Anti-immunoglobulins in multiple myeloma and benign monoclonal hyperglobulinemia]. Sera from 50 healthy adults, from 21 patients with multiple myeloma (MM) and from 11 patients with benign monoclonal hyperglobulinaemia (BMH) and from 28 patients with sarcoidosis were examined for the presence of anti-IgG-activity by passive haemagglutination technique. 62% of healthy adults (titre less than 2) and all of the sera from patients with MM and BMH (titres 32-512) as well as 42% of the sera from sarcoidosis patients were found to be anti-IgG positive. The anti-IgG positive sera showed also anti-(Fab)2-activity (with the exeption of 2 sera from sarcoidosis patients). No significant differences could be found between anti-Ig activity in sera from MM patients with BMH. There was also seen no correlation of anti-Ig with the clinical course of the disease. After column chromatography we could detect the partially simultaneous presence of anti-Ig with anti-Fc-specificity (MW ca. 900,000) and with (Fab)2-specificity (MW 150,000 or less than 90,000). Antibody dependent cytotoxicity (using melanoma cell lines as targets) was significantly inhibited by the isolated anti-Ig-fractions with low molecular sizes (MW less than 90,000). From these results it seems possible that anti-immunoglobulins may play a role in the clinical course of the disease."}
{"id": "PMID:1083268", "title": "[Colony-stimulating activity in urine and serum in a child with cyclic neutropenia].", "content": "Colony stimulating activity (CSA) was estimated in daily urine samples and weekly plasma specimens of a 4-year-old girl with familial cyclic neutropenia. Nadirs of neutrophils and peaks of monocytes were found in regular cycles of 28 days. A close correlation was observed between nadirs of neutrophils and peaks of blood monocytes and colony stimulating activity in the urine. The role of colony stimulating factor as regulatory factor in granulopoiesis in cyclic neutropenia is discussed.", "contents": "[Colony-stimulating activity in urine and serum in a child with cyclic neutropenia]. Colony stimulating activity (CSA) was estimated in daily urine samples and weekly plasma specimens of a 4-year-old girl with familial cyclic neutropenia. Nadirs of neutrophils and peaks of monocytes were found in regular cycles of 28 days. A close correlation was observed between nadirs of neutrophils and peaks of blood monocytes and colony stimulating activity in the urine. The role of colony stimulating factor as regulatory factor in granulopoiesis in cyclic neutropenia is discussed."}
{"id": "PMID:1083269", "title": "[Effect of cordycepine on cultured chicken fibroblasts].", "content": "Cordycepin strongly inhibits cell multiplication in chick fibroblasts cultivated in vitro. Many cells cannot enter into mitosis and cell separation at telophase is disturbed. However, DNA synthesis takes place. Nucleolar alterations observed in the treated cells are probably responsible for some of these effects.", "contents": "[Effect of cordycepine on cultured chicken fibroblasts]. Cordycepin strongly inhibits cell multiplication in chick fibroblasts cultivated in vitro. Many cells cannot enter into mitosis and cell separation at telophase is disturbed. However, DNA synthesis takes place. Nucleolar alterations observed in the treated cells are probably responsible for some of these effects."}
{"id": "PMID:1083271", "title": "Isotonic and isometric responses of different tonic muscles to agonists and antagonists.", "content": "1 With isotonic recording the percentage of muscle shortening as compared with the maximal possible shortening, and with isometric recording the percentage of developed tension were determined. In relatively 'thick' muscles, such as dorsal leech muscle, frog rectus abdominis or protractor pharynx of holothuria (0.3-0.8 mm thick), the concentrations of a full agonist (carbachol) producing a given percentage of tension, (e.g. 50%) are about 5 times greater than the concentrations, producing the same percentage of shortening. In 'thin' muscles the difference between the percentage of shortening and tension is either small (retractor dentis of the sea urchin, 0.1 mm thick, response to carbachol) or absent (guinea-pig ileum, 0.06 mm thick, responses to methylfurmethide). The possible mechanism underlying this difference is discussed. 2 With partial agonists (dodecamethonium and heptamethonium) the fractional tension of the frog rectus abdominis is always less than the fractional shortening and the correlation between shortening and tension is the same as in the case of full agonists. 3 The blocking activity of (+)-tubocurarine on the frog rectus abdominis is the same in isotonic and in isometric conditions. 4 On the frog rectus abdominis the alkylating agent, decamethonium mustard, does not produce any 'parallel shift' of the dose-response curve for carbachol, the only result of alkylation being a decrease in maximal response, which is more pronounced in isometric than in isotonic conditions. The degree of decrease is in accordance with the correlation between percentage of shortening and percentage of tension in the absence of alkylating agent. Probably this muscle does not possess any 'spare receptors'. 5 On the frog muscle the dose-isometric response curve for acetylcholine (ACh) is shifted toward greater concentration about 33-fold as compared with the dose-isotonic response curve but after the inhibition of cholinesterases the shift is only about 6-fold. The same shift (5-fold) is observed for carbachol, which is not hydrolysed by cholinesterases. The results with ACh are due to the fact, that after cholinesterase inhibition the sensitivity to ACh increases in isotonic conditions only 13-fold, but in isometric conditions it increases 71-fold. Probably under isometric conditions, when the muscle remains in the extended state, the rate of hydrolysis of ACh is much greater than under isotonic conditions when the muscle is shortened during contraction.", "contents": "Isotonic and isometric responses of different tonic muscles to agonists and antagonists. 1 With isotonic recording the percentage of muscle shortening as compared with the maximal possible shortening, and with isometric recording the percentage of developed tension were determined. In relatively 'thick' muscles, such as dorsal leech muscle, frog rectus abdominis or protractor pharynx of holothuria (0.3-0.8 mm thick), the concentrations of a full agonist (carbachol) producing a given percentage of tension, (e.g. 50%) are about 5 times greater than the concentrations, producing the same percentage of shortening. In 'thin' muscles the difference between the percentage of shortening and tension is either small (retractor dentis of the sea urchin, 0.1 mm thick, response to carbachol) or absent (guinea-pig ileum, 0.06 mm thick, responses to methylfurmethide). The possible mechanism underlying this difference is discussed. 2 With partial agonists (dodecamethonium and heptamethonium) the fractional tension of the frog rectus abdominis is always less than the fractional shortening and the correlation between shortening and tension is the same as in the case of full agonists. 3 The blocking activity of (+)-tubocurarine on the frog rectus abdominis is the same in isotonic and in isometric conditions. 4 On the frog rectus abdominis the alkylating agent, decamethonium mustard, does not produce any 'parallel shift' of the dose-response curve for carbachol, the only result of alkylation being a decrease in maximal response, which is more pronounced in isometric than in isotonic conditions. The degree of decrease is in accordance with the correlation between percentage of shortening and percentage of tension in the absence of alkylating agent. Probably this muscle does not possess any 'spare receptors'. 5 On the frog muscle the dose-isometric response curve for acetylcholine (ACh) is shifted toward greater concentration about 33-fold as compared with the dose-isotonic response curve but after the inhibition of cholinesterases the shift is only about 6-fold. The same shift (5-fold) is observed for carbachol, which is not hydrolysed by cholinesterases. The results with ACh are due to the fact, that after cholinesterase inhibition the sensitivity to ACh increases in isotonic conditions only 13-fold, but in isometric conditions it increases 71-fold. Probably under isometric conditions, when the muscle remains in the extended state, the rate of hydrolysis of ACh is much greater than under isotonic conditions when the muscle is shortened during contraction."}
{"id": "PMID:1083274", "title": "Fetal complications of obstetric cholestasis.", "content": "Among 56 pregnancies complicated by obstetric cholestasis five intrauterine deaths and one neonatal death occurred between 33 and 39 weeks, and a further six infants required urgent delivery for intrapartum asphyxia. Eighteen spontaneous premature deliveries occurred. Five mothers required specific treatment for unexplained postpartum haemorrhage. Cholestasis of pregnancy is therefore not a condition benign to the fetus, and it may contribute to increased maternal morbidity.", "contents": "Fetal complications of obstetric cholestasis. Among 56 pregnancies complicated by obstetric cholestasis five intrauterine deaths and one neonatal death occurred between 33 and 39 weeks, and a further six infants required urgent delivery for intrapartum asphyxia. Eighteen spontaneous premature deliveries occurred. Five mothers required specific treatment for unexplained postpartum haemorrhage. Cholestasis of pregnancy is therefore not a condition benign to the fetus, and it may contribute to increased maternal morbidity."}
{"id": "PMID:1083275", "title": "Study of female babies of women entering confinement with vaginal trichomoniasis.", "content": "A series of 868 women in the last 4 weeks of pregnancy were examined for the presence of T. vaginalis by direct microscopy of freshly prepared wet films and by three culture methods; 32 (3-7 per cent.) were found to give positive results. Fourteen of the mothers with trichomoniasis at confinement had female babies, and these, together with a matching fourteen controls, were examined on three occasions by identical methods and within 21 days of birth. No baby gave positive results. On statistical examination it is very unlikely that with a much larger sample the number of positive findings could have been greater than 23 per cent. This is at variance with recent Polish work. These results suggest that neonatal infection in female babies, although a recognized clinical entity, is rare and unlikely to make any substantial contribution to the high incidence of trichomoniasis in women.", "contents": "Study of female babies of women entering confinement with vaginal trichomoniasis. A series of 868 women in the last 4 weeks of pregnancy were examined for the presence of T. vaginalis by direct microscopy of freshly prepared wet films and by three culture methods; 32 (3-7 per cent.) were found to give positive results. Fourteen of the mothers with trichomoniasis at confinement had female babies, and these, together with a matching fourteen controls, were examined on three occasions by identical methods and within 21 days of birth. No baby gave positive results. On statistical examination it is very unlikely that with a much larger sample the number of positive findings could have been greater than 23 per cent. This is at variance with recent Polish work. These results suggest that neonatal infection in female babies, although a recognized clinical entity, is rare and unlikely to make any substantial contribution to the high incidence of trichomoniasis in women."}
{"id": "PMID:1083276", "title": "Rapidly transported proteins in sensory, motor and sympathetic nerves of the isolated frog nervous system.", "content": "The synthesis and rapid axonal transport of [35S]methionine-labelled proteins has been studied using the isolated frog spinal cord and peripheral nervous system. Polyaerylamide gel electrophoresis in sodium dodecylsulfate of synthesized and transported proteins revealed similar labelling patterns of proteins transported in the sensory, motor and sympathetic systems. The relative labelling pattern of transported proteins which accumulated at ligatures in peripheral nerves was different from those obtained from ganglia or nerves and roots when they were incubated in labelled methionine. When compared with methionine-labelled protein profiles of rapid axonal transport in other species and systems, a common set of rapidly transported proteins emerges. The approximate molecular weights of these common proteins include (in 1000 daltons): 18,24-29, 34-36, 57, 65-68, 100 and 130. These proteins may represent fundamental macromolecules involved in the general maintenance of the function of nerve processes.", "contents": "Rapidly transported proteins in sensory, motor and sympathetic nerves of the isolated frog nervous system. The synthesis and rapid axonal transport of [35S]methionine-labelled proteins has been studied using the isolated frog spinal cord and peripheral nervous system. Polyaerylamide gel electrophoresis in sodium dodecylsulfate of synthesized and transported proteins revealed similar labelling patterns of proteins transported in the sensory, motor and sympathetic systems. The relative labelling pattern of transported proteins which accumulated at ligatures in peripheral nerves was different from those obtained from ganglia or nerves and roots when they were incubated in labelled methionine. When compared with methionine-labelled protein profiles of rapid axonal transport in other species and systems, a common set of rapidly transported proteins emerges. The approximate molecular weights of these common proteins include (in 1000 daltons): 18,24-29, 34-36, 57, 65-68, 100 and 130. These proteins may represent fundamental macromolecules involved in the general maintenance of the function of nerve processes."}
{"id": "PMID:1083278", "title": "Myocardial infarction after aortocoronary bypass: etiologic factors.", "content": "To ascertain the frequency and probable determinants of myocardial infarction after aortocoronary bypass, two groups of patients were studied: a group of 20 patients who underwent valve replacement of repair of congenital anomalies and a group of 24 patients who underwent aortocoronary bypass. Postoperative myocardial infarction was diagnosed by an increase in serum creatine phosphokinase concentration to a minimum of 1200 IU in two consecutive samples. The frequency of myocardial infarction differed between the groups, being only 5% in group 1 but 20.4% in group 2. In the patients undergoing coronary artery surgery, the duration of bypass, the aortic cross-clamp time and the vent site did not influence the rate of infarction. However, the incidence of myocardial necrosis did correlate with the number of vessels bypassed. In no case was the clinical course influenced by the infarction. Our results suggest that the frequency of perioperative infarction is higher in coronary artery surgery than in other adult cardiac surgery, and that the most important determining factor is the number of diseased vessels.", "contents": "Myocardial infarction after aortocoronary bypass: etiologic factors. To ascertain the frequency and probable determinants of myocardial infarction after aortocoronary bypass, two groups of patients were studied: a group of 20 patients who underwent valve replacement of repair of congenital anomalies and a group of 24 patients who underwent aortocoronary bypass. Postoperative myocardial infarction was diagnosed by an increase in serum creatine phosphokinase concentration to a minimum of 1200 IU in two consecutive samples. The frequency of myocardial infarction differed between the groups, being only 5% in group 1 but 20.4% in group 2. In the patients undergoing coronary artery surgery, the duration of bypass, the aortic cross-clamp time and the vent site did not influence the rate of infarction. However, the incidence of myocardial necrosis did correlate with the number of vessels bypassed. In no case was the clinical course influenced by the infarction. Our results suggest that the frequency of perioperative infarction is higher in coronary artery surgery than in other adult cardiac surgery, and that the most important determining factor is the number of diseased vessels."}
{"id": "PMID:1083279", "title": "Prognostic significance of endocardial viability ratio in aortocoronary bypass surgery.", "content": "Although aortocoronary bypass (ACB) for patients with stable angina carries a low mortality, some unexpected deaths do occur. Since in patients with normal coronary arteries the endocardial viability ratio (EVR) can be correlated with subendocardial perfusion, with a ratio of 0.7 or less indicating ischemia of the left ventricular subendocardium, and since the EVR postoperatively is useful in determining the need for intra-aortic balloon pump assist (IABPA), it was decided to ascertain whether the EVR might have prognostic value in patients with stable angina scheduled for standard ACB. Three groups of patients were studied: 50 with stable angina, 24 who had died after ACB, and 18 who required IABPA for cardiogenic shock after surgery for stable angina. No significant differences were found for cardiac index, left ventricular end-diastolic pressure, left ventricular ejection fraction and pre- and postoperative artery scores, but there were significant differences in the EVR between the first and second groups and between the first and third groups (P less than 0.01 in each instance). These findings suggest that the left ventricular EVR may indeed be of prognostic value in patients scheduled to undergo ACB and that use of IABPA, which produces an increase in EVR, may be useful in patients with EVRs of less than 0.7, even if other parameters of cardiac function are normal.", "contents": "Prognostic significance of endocardial viability ratio in aortocoronary bypass surgery. Although aortocoronary bypass (ACB) for patients with stable angina carries a low mortality, some unexpected deaths do occur. Since in patients with normal coronary arteries the endocardial viability ratio (EVR) can be correlated with subendocardial perfusion, with a ratio of 0.7 or less indicating ischemia of the left ventricular subendocardium, and since the EVR postoperatively is useful in determining the need for intra-aortic balloon pump assist (IABPA), it was decided to ascertain whether the EVR might have prognostic value in patients with stable angina scheduled for standard ACB. Three groups of patients were studied: 50 with stable angina, 24 who had died after ACB, and 18 who required IABPA for cardiogenic shock after surgery for stable angina. No significant differences were found for cardiac index, left ventricular end-diastolic pressure, left ventricular ejection fraction and pre- and postoperative artery scores, but there were significant differences in the EVR between the first and second groups and between the first and third groups (P less than 0.01 in each instance). These findings suggest that the left ventricular EVR may indeed be of prognostic value in patients scheduled to undergo ACB and that use of IABPA, which produces an increase in EVR, may be useful in patients with EVRs of less than 0.7, even if other parameters of cardiac function are normal."}
{"id": "PMID:1083280", "title": "Triple-vessel coronary artery disease: Effect of revascularization on late survival.", "content": "Of the first 600 patients to receive aortocoronary bypass grafts 383 had three diseased vessels, and of them 36 (9%) have died: 20 (5%) in hospital and 16 (4%) late. In 10 of those who died, ventricular function was normal, class I or class II and in the other 26 function was class III or class IV. For all ventricular classes except class IV, improvement in myocardial blood flow with operation was considerably lower than average in those who died. The leading cause of late death was congestive heart failure followed by myocardial infarction, and then by other cardiac complications. Of patients with good (normal, classes I or II) ventricular function, 3.5% died during a mean follow-up period of 27 months; and since, according to published reports, 1% of patients with triple-vessel disease without surgical intervention die each month, the data from this study suggest that survival in this group is improved by 23.5%. This study also shows that  in patients with triple-vessel disease and good ventricular function, adequate coronary artery bypass operations (i.e., placement of three grafts or more) improves survival.", "contents": "Triple-vessel coronary artery disease: Effect of revascularization on late survival. Of the first 600 patients to receive aortocoronary bypass grafts 383 had three diseased vessels, and of them 36 (9%) have died: 20 (5%) in hospital and 16 (4%) late. In 10 of those who died, ventricular function was normal, class I or class II and in the other 26 function was class III or class IV. For all ventricular classes except class IV, improvement in myocardial blood flow with operation was considerably lower than average in those who died. The leading cause of late death was congestive heart failure followed by myocardial infarction, and then by other cardiac complications. Of patients with good (normal, classes I or II) ventricular function, 3.5% died during a mean follow-up period of 27 months; and since, according to published reports, 1% of patients with triple-vessel disease without surgical intervention die each month, the data from this study suggest that survival in this group is improved by 23.5%. This study also shows that  in patients with triple-vessel disease and good ventricular function, adequate coronary artery bypass operations (i.e., placement of three grafts or more) improves survival."}
{"id": "PMID:1083283", "title": "Life tables as \"predictors\" of average longevity.", "content": "Selected figures from Canadian life tables have been analysed to illustrate one potential use of routinely collected and published data. A plea is made for the inclusion of the fundamentals of demography in undergraduate medical education.", "contents": "Life tables as \"predictors\" of average longevity. Selected figures from Canadian life tables have been analysed to illustrate one potential use of routinely collected and published data. A plea is made for the inclusion of the fundamentals of demography in undergraduate medical education."}
{"id": "PMID:1083285", "title": "The effect of radiation therapy on lymphocyte subpopulations in cancer patients.", "content": "The proportion of T and B lymphocytes in the peripheral blood was determined in patients with either mammary cancer or with various pelvic malignancies. In cancer patients studied prior to irradiation the level of cells forming either E-rosettes or EAC'-rosettes was similar to that found among healthy controls. Radiation therapy resulted in a striking lymphopenia. The level of cells with T-cell markers was diminished to a greater extent than the level of cells with B-cell markers. The relative proportion of T-cells forming high affinity E-rosettes was not reduced following radiation, so that it can be concluded that radiation affects predominantly the subpopulation of T-cells which do not form high affinity E-rosettes. Irradiation of the pelvic area resulted in a more rapid reduction of the level of T lymphocytes than irradiation of the mediastinum, although the final relative proportions of the cells were similar in both groups of patients. The results of the present study suggest that the reduction of the level of T lymphocytes following irradiation results from its effect on the lymphocytes in the major blood vessels, and that radiation of the thymus is not a prerequisite for this phenomenon.", "contents": "The effect of radiation therapy on lymphocyte subpopulations in cancer patients. The proportion of T and B lymphocytes in the peripheral blood was determined in patients with either mammary cancer or with various pelvic malignancies. In cancer patients studied prior to irradiation the level of cells forming either E-rosettes or EAC'-rosettes was similar to that found among healthy controls. Radiation therapy resulted in a striking lymphopenia. The level of cells with T-cell markers was diminished to a greater extent than the level of cells with B-cell markers. The relative proportion of T-cells forming high affinity E-rosettes was not reduced following radiation, so that it can be concluded that radiation affects predominantly the subpopulation of T-cells which do not form high affinity E-rosettes. Irradiation of the pelvic area resulted in a more rapid reduction of the level of T lymphocytes than irradiation of the mediastinum, although the final relative proportions of the cells were similar in both groups of patients. The results of the present study suggest that the reduction of the level of T lymphocytes following irradiation results from its effect on the lymphocytes in the major blood vessels, and that radiation of the thymus is not a prerequisite for this phenomenon."}
{"id": "PMID:1083286", "title": "Failure of leucovorin rescue to prevent reactivation of a solar burn after high dose methotrexate.", "content": "A 21-year-old patient with metastatic osteosarcoma was receiving methotrexate with leucovorin rescue every 2 weeks. After the second (of four) infusions of methotrexate, a prior solar burn on an area of skin was reactivated in spite of leucovorin rescue. An area of skin treated 5 months previously by radiation was spared the effects of the reactivation phenomenon. No other toxicities appeared. The reactivation of the solar burn is an example of \"false photosensitization\" and this cutaneous toxicity is not ameliorated by leucovorin. Methotrexate therapy should be delayed until the effects of generalized solar burns have resolved (approximately 1 week).", "contents": "Failure of leucovorin rescue to prevent reactivation of a solar burn after high dose methotrexate. A 21-year-old patient with metastatic osteosarcoma was receiving methotrexate with leucovorin rescue every 2 weeks. After the second (of four) infusions of methotrexate, a prior solar burn on an area of skin was reactivated in spite of leucovorin rescue. An area of skin treated 5 months previously by radiation was spared the effects of the reactivation phenomenon. No other toxicities appeared. The reactivation of the solar burn is an example of \"false photosensitization\" and this cutaneous toxicity is not ameliorated by leucovorin. Methotrexate therapy should be delayed until the effects of generalized solar burns have resolved (approximately 1 week)."}
{"id": "PMID:1083287", "title": "Tumor cell characterization in mycosis fungoides.", "content": "Five patients with tumor stage mycosis fungoides had tumor lesions excised and examined by light and electron microscopy. The tumor cells were also isolated and the percentages of E rosettes (T cells) and EAC rosettes (B cells) were determined and the rosettes examined by electron microscopy. The predominant cell type isolated from four of the five tumors was a T cell. Peripheral lymphocyte function measured by LTT to phytohemagglutinin and pokeweed mitogen was normal. Serum immunoglobulins gave variable results. The results support the concept that mycosis fungoides is primarily a T cell tumor involving the skin and morphologically similar to the S\u00e9zary cell.", "contents": "Tumor cell characterization in mycosis fungoides. Five patients with tumor stage mycosis fungoides had tumor lesions excised and examined by light and electron microscopy. The tumor cells were also isolated and the percentages of E rosettes (T cells) and EAC rosettes (B cells) were determined and the rosettes examined by electron microscopy. The predominant cell type isolated from four of the five tumors was a T cell. Peripheral lymphocyte function measured by LTT to phytohemagglutinin and pokeweed mitogen was normal. Serum immunoglobulins gave variable results. The results support the concept that mycosis fungoides is primarily a T cell tumor involving the skin and morphologically similar to the S\u00e9zary cell."}
{"id": "PMID:1083288", "title": "T and B lymphocytes in non-Hodgkin's lymphoma: a comparison of tumor-derived cells and peripheral blood lymphocytes.", "content": "T- and B-cell markers of lymphocytes in peripheral blood, involved node and spleen, PHA response of peripheral blood lymphocytes, serum immunoglobulin levels, and skin test reactivity to six common antigens were studied in 16 cases of untreated non-Hodgkin's lymphoma. Impaired response of peripheral lymphocytes to PHA was observed in 13 of 16 cases, regardless of the proportion of T lymphocytes. Of 12 cases in which skin tests were done, two were positive and had a normal PHA response, seven cases were positive in spite of low PHA response, and three were negative with low PHA response. In the lymph nodes from involved areas two cases showed monoclonal increase of B-cells, five showed \"null\" cell increase, and the remaining nine showed no increase or decrease of subpopulation of lymphocytes. No correlation with surface marker of lymphocytes to histologic classification was seen. From the above observations it was concluded: 1) a low PHA response in non-Hodgkin's lymphoma was not due to the decreased population of T-cells; 2) a low PHA response may not necessarily indicate impaired delayed hypersensitivity; and 3) non-Hodgkin's lymphoma can be classified in the following ways--B-cell proliferative type, \"null\" cell increase type, and normal T/B proportion type.", "contents": "T and B lymphocytes in non-Hodgkin's lymphoma: a comparison of tumor-derived cells and peripheral blood lymphocytes. T- and B-cell markers of lymphocytes in peripheral blood, involved node and spleen, PHA response of peripheral blood lymphocytes, serum immunoglobulin levels, and skin test reactivity to six common antigens were studied in 16 cases of untreated non-Hodgkin's lymphoma. Impaired response of peripheral lymphocytes to PHA was observed in 13 of 16 cases, regardless of the proportion of T lymphocytes. Of 12 cases in which skin tests were done, two were positive and had a normal PHA response, seven cases were positive in spite of low PHA response, and three were negative with low PHA response. In the lymph nodes from involved areas two cases showed monoclonal increase of B-cells, five showed \"null\" cell increase, and the remaining nine showed no increase or decrease of subpopulation of lymphocytes. No correlation with surface marker of lymphocytes to histologic classification was seen. From the above observations it was concluded: 1) a low PHA response in non-Hodgkin's lymphoma was not due to the decreased population of T-cells; 2) a low PHA response may not necessarily indicate impaired delayed hypersensitivity; and 3) non-Hodgkin's lymphoma can be classified in the following ways--B-cell proliferative type, \"null\" cell increase type, and normal T/B proportion type."}
{"id": "PMID:1083289", "title": "The accumulated effects of repeated systemic or local injections of low doses of Corynebacterium parvum in mice.", "content": "The effects of 14 weekly injections, s.c. or i.v., of \"human equivalent\" doses (5.25 mg/sq m) of Corynebacterium parvum (CP) in mice have been compared. Both s.c. and i.v. CP caused significant splenomegaly and antibody to CP, but stimulation was considerably greater after i.v. CP. Delayed hypersensitivity levels to CP were similar after s.c. and i.v. injection. T-cell competence, as judged by phytohemagglutinin reactivity and delayed hypersensitivity to sheep cells, was unimpaired after s.c. CP and augmented by i.v. CP. Activated peritoneal macrophages capable of nonspecifically inhibiting tumor growth in vitro were detected only after i.v. CP, and in vivo resistance to tumor cell challenge was greater after CP administered i.v. than s.c.", "contents": "The accumulated effects of repeated systemic or local injections of low doses of Corynebacterium parvum in mice. The effects of 14 weekly injections, s.c. or i.v., of \"human equivalent\" doses (5.25 mg/sq m) of Corynebacterium parvum (CP) in mice have been compared. Both s.c. and i.v. CP caused significant splenomegaly and antibody to CP, but stimulation was considerably greater after i.v. CP. Delayed hypersensitivity levels to CP were similar after s.c. and i.v. injection. T-cell competence, as judged by phytohemagglutinin reactivity and delayed hypersensitivity to sheep cells, was unimpaired after s.c. CP and augmented by i.v. CP. Activated peritoneal macrophages capable of nonspecifically inhibiting tumor growth in vitro were detected only after i.v. CP, and in vivo resistance to tumor cell challenge was greater after CP administered i.v. than s.c."}
{"id": "PMID:1083290", "title": "Secondary in vitro lymphocyte-proliferative responses to syngeneic plasma cell tumors.", "content": "Two characteristics of immune responses to weakly immunogenic plasma cell tumors were demonstrated in this study. (a) Elevated lymphoproliferative responses following in vivo inoculation of sublethal doses of plasma cell tumors were detected by a mixed lymphocyte-tumor interaction (MLTI) assay. Specificity of elevated MLTI responses correlated with resistance to in vivo tumor challenge. These responses were dependent upon the presence of thymus-derived cells. (b) Spleen and lymph node cells showed markedly different patterns of MLTI activity following plasma cell tumor immunization. Spleen cell responses were depressed for the first 30 days postimmunization, whereas lymph node cells showed augmented MLTI reactivity immediately after immunization. These observations indicated that lymphocyte populations from different lymphoid organs may show widely dissimilar responses in vitro at the same point in the in vivo development of tumor resistance.", "contents": "Secondary in vitro lymphocyte-proliferative responses to syngeneic plasma cell tumors. Two characteristics of immune responses to weakly immunogenic plasma cell tumors were demonstrated in this study. (a) Elevated lymphoproliferative responses following in vivo inoculation of sublethal doses of plasma cell tumors were detected by a mixed lymphocyte-tumor interaction (MLTI) assay. Specificity of elevated MLTI responses correlated with resistance to in vivo tumor challenge. These responses were dependent upon the presence of thymus-derived cells. (b) Spleen and lymph node cells showed markedly different patterns of MLTI activity following plasma cell tumor immunization. Spleen cell responses were depressed for the first 30 days postimmunization, whereas lymph node cells showed augmented MLTI reactivity immediately after immunization. These observations indicated that lymphocyte populations from different lymphoid organs may show widely dissimilar responses in vitro at the same point in the in vivo development of tumor resistance."}
{"id": "PMID:1083292", "title": "Ultrastructural studies on neurophysine-containing vesicles of the neurosecretory system of vertebrates.", "content": "A new method described in the present paper allows identification of neurophysine-containing (NP) vesicles of the magnocellular neurosecretory system of vertebrates. After oxidation of ultrathin sections, the content of NP vesicles can be dissolved specifically in an alkaline medium. This procedure marks NP vesicles selectively in all portions of the classical neurosecretory system.", "contents": "Ultrastructural studies on neurophysine-containing vesicles of the neurosecretory system of vertebrates. A new method described in the present paper allows identification of neurophysine-containing (NP) vesicles of the magnocellular neurosecretory system of vertebrates. After oxidation of ultrathin sections, the content of NP vesicles can be dissolved specifically in an alkaline medium. This procedure marks NP vesicles selectively in all portions of the classical neurosecretory system."}
{"id": "PMID:1083293", "title": "Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta). II. An in vitro study of the transport process and the influence of temperature.", "content": "Frog pancreatic tissue was pulse-labelled in vitro with 3H-leucine and protein transport was studied in exocrine cells by electron microscope autoradiography. The proteins appeared to be synthesized in the RER and transported to the secretory granules along a similar route and with the same velocity as previously described under in vitro conditions. Evidence was obtained for the involvement of the vesicular and tubular elements at the periphery of the Golgi system in transferring protein from the RER to the Golgi cisternae. Kinetics of the release of newly synthesized proteins from the RER and their appearance in the condensing vacuoles are discussed and related to results reported from other tissues. The transport velocity in this poikilothermic system was studied in relation to the incubation temperature and compared with results reported from its mammalian counterpart. At temperatures between 20 and 30 degrees C intracellular protein transport occurs faster in the frog than in the Guinea pig pancreas. At higher temperature the transport process was severely disturbed in the frog.", "contents": "Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta). II. An in vitro study of the transport process and the influence of temperature. Frog pancreatic tissue was pulse-labelled in vitro with 3H-leucine and protein transport was studied in exocrine cells by electron microscope autoradiography. The proteins appeared to be synthesized in the RER and transported to the secretory granules along a similar route and with the same velocity as previously described under in vitro conditions. Evidence was obtained for the involvement of the vesicular and tubular elements at the periphery of the Golgi system in transferring protein from the RER to the Golgi cisternae. Kinetics of the release of newly synthesized proteins from the RER and their appearance in the condensing vacuoles are discussed and related to results reported from other tissues. The transport velocity in this poikilothermic system was studied in relation to the incubation temperature and compared with results reported from its mammalian counterpart. At temperatures between 20 and 30 degrees C intracellular protein transport occurs faster in the frog than in the Guinea pig pancreas. At higher temperature the transport process was severely disturbed in the frog."}
{"id": "PMID:1083294", "title": "A clinically useful coding system for normal coronary artery anatomy.", "content": "Cine coronary arteriograms of 121 patients with normal coronary arteriograms with and without associated valvular or myocardial disease were reviewed to describe normal coronary artery anatomy and the distribution of potentially graftable vessels. Coronary arterio grams were divided into right, mixed, and left interior emphasis systems, depending upon the blood supply to the inferior surface of the left ventricle. Whether a coronary artery at its origin was less than 50%, between 50-70% of the left anterior descending coronary artery (LAD) at its origin was described in a simple lettering and numbering code to allow easy comparison. The diameter of the LAD at its origin averaged 4.2 mm. All vessels greater than 70% of the LAD were clearly graftable. The frequency (in percent) with which that vessel was greater than 70% of the LAD in right, mixed, and left systems, respectively, was as follows: right coronary artery 100. 100. 0. LAD 100. 100. 100. intermediate artery 8.7 12.5 33.3 obtuse marginal artery 53.7 43.7 44.4 atrioventricular groove artery 0. 56.2 100. diagonal branch of the LAD 15. 6.3 11.1. This simple method of classification is recommended to provide more accurate comparison in published series and to assist the coronary arteriographer in his interpretations.", "contents": "A clinically useful coding system for normal coronary artery anatomy. Cine coronary arteriograms of 121 patients with normal coronary arteriograms with and without associated valvular or myocardial disease were reviewed to describe normal coronary artery anatomy and the distribution of potentially graftable vessels. Coronary arterio grams were divided into right, mixed, and left interior emphasis systems, depending upon the blood supply to the inferior surface of the left ventricle. Whether a coronary artery at its origin was less than 50%, between 50-70% of the left anterior descending coronary artery (LAD) at its origin was described in a simple lettering and numbering code to allow easy comparison. The diameter of the LAD at its origin averaged 4.2 mm. All vessels greater than 70% of the LAD were clearly graftable. The frequency (in percent) with which that vessel was greater than 70% of the LAD in right, mixed, and left systems, respectively, was as follows: right coronary artery 100. 100. 0. LAD 100. 100. 100. intermediate artery 8.7 12.5 33.3 obtuse marginal artery 53.7 43.7 44.4 atrioventricular groove artery 0. 56.2 100. diagonal branch of the LAD 15. 6.3 11.1. This simple method of classification is recommended to provide more accurate comparison in published series and to assist the coronary arteriographer in his interpretations."}
{"id": "PMID:1083295", "title": "An additional angiographic sign for determining coronary artery dominance in obstructive coronary artery disease.", "content": "An additional angiographic sign for determining coronary artery dominance is proposed. This sign is based on the anatomic pattern of the infraventricular branches in the left anterior oblique view. It is especially helpful in patients in whom the right coronary and/or left circumflex coronary arteries are completely occluded and distal vessels are filled via collaterals.", "contents": "An additional angiographic sign for determining coronary artery dominance in obstructive coronary artery disease. An additional angiographic sign for determining coronary artery dominance is proposed. This sign is based on the anatomic pattern of the infraventricular branches in the left anterior oblique view. It is especially helpful in patients in whom the right coronary and/or left circumflex coronary arteries are completely occluded and distal vessels are filled via collaterals."}
{"id": "PMID:1083296", "title": "Preoperative coronary angiographic prediction of bypass flow and short-term patency.", "content": "A quantitative preoperative coronary angiographic index was defined in 148 patients undergoing coronary revascularization. Each diseased vessel was scored 0-3, for both diameter and quality of run-off. The sum of the scores for diameter and run-off constituted the numerical index for the diseased vessel. Correlations between the index and graft flow measured by electromagnetic flow meter at surgery were established in 259 bypassed vessels. The highest scores (5-6) were associated with higher flows, and the lower scores (0-4) with the lower flows (P less than 0.005). Repeat angiography performed 2 wk postoperatively in 110 patients demonstrated 174 graft patencies and 15 graft closures. Mean flow in open grafts was 82 +/- 41 ml/mn vs. 60 +/- 23ml/mn in closed grafts (P less than 0.005). It is concluded that graft flow is predictable from preoperative Coronary Angiographic Index and that higher flow and index scores are more likely to be associated with graft patency than low flows and index scores.", "contents": "Preoperative coronary angiographic prediction of bypass flow and short-term patency. A quantitative preoperative coronary angiographic index was defined in 148 patients undergoing coronary revascularization. Each diseased vessel was scored 0-3, for both diameter and quality of run-off. The sum of the scores for diameter and run-off constituted the numerical index for the diseased vessel. Correlations between the index and graft flow measured by electromagnetic flow meter at surgery were established in 259 bypassed vessels. The highest scores (5-6) were associated with higher flows, and the lower scores (0-4) with the lower flows (P less than 0.005). Repeat angiography performed 2 wk postoperatively in 110 patients demonstrated 174 graft patencies and 15 graft closures. Mean flow in open grafts was 82 +/- 41 ml/mn vs. 60 +/- 23ml/mn in closed grafts (P less than 0.005). It is concluded that graft flow is predictable from preoperative Coronary Angiographic Index and that higher flow and index scores are more likely to be associated with graft patency than low flows and index scores."}
{"id": "PMID:1083297", "title": "105 mm photofluorography: an adjunct to the study of coronary arterial and vascular disease.", "content": "Obtaining high resolution static films through an image intensifier has been made possible by recent developments in radiographic equipment. Serial photofluorography 105 mm spot filming has been used successfully to complement cine coronary arteriography in approximately 700 patients. Many patients with coronary heart disease have diffuse peripheral atheromatous changes which are also suitable to study by serial 105 mm photofluorography. Aortic arch, abdominal aortography, and peripheral studies are easily and quickly performed in conjunction with coronary arteriography. Satisfactory results still depend upon skillful application by the angiographer of this imaging mode and proper processing of the film. Advantages of photofluorographic filming over conventional large film techniques include lower radiation exposure, reduction of costs, and easier film handling and storage. Most important is the facilitation of the performance of angiographic studies since constant television monitoring of the catheter and the contrast injections is possible. Disadvantages include restricted area of coverage and slight decrease in resolution. Our experience indicates that photofluorography may be relied upon in the study of vascular disease eliminating the need for large film changers in a laboratory used primarily for cardiac diagnosis.", "contents": "105 mm photofluorography: an adjunct to the study of coronary arterial and vascular disease. Obtaining high resolution static films through an image intensifier has been made possible by recent developments in radiographic equipment. Serial photofluorography 105 mm spot filming has been used successfully to complement cine coronary arteriography in approximately 700 patients. Many patients with coronary heart disease have diffuse peripheral atheromatous changes which are also suitable to study by serial 105 mm photofluorography. Aortic arch, abdominal aortography, and peripheral studies are easily and quickly performed in conjunction with coronary arteriography. Satisfactory results still depend upon skillful application by the angiographer of this imaging mode and proper processing of the film. Advantages of photofluorographic filming over conventional large film techniques include lower radiation exposure, reduction of costs, and easier film handling and storage. Most important is the facilitation of the performance of angiographic studies since constant television monitoring of the catheter and the contrast injections is possible. Disadvantages include restricted area of coverage and slight decrease in resolution. Our experience indicates that photofluorography may be relied upon in the study of vascular disease eliminating the need for large film changers in a laboratory used primarily for cardiac diagnosis."}
{"id": "PMID:1083303", "title": "Characteristics of pregnant women who report previous induced abortions.", "content": "Associations between previous induced abortion and demographic and health factors in pregnancy were measured in 9 874 women who gave birth and who had been interviewed during pregnancy. Previous abortion was most rare among women having their first baby and increased with increasing birth order up to the fourth, thereafter decreasing. It was positively correlated with maternal age and negatively with age at marriage. There was no effect of years of schooling, when other variables were taken into account, but there were significant differences between ethnic groups, abortion being commonest among Jewish women from North African countries and more prevalent in those from western and Asian countries than in the second-generation Israel-born or in Arab women.Women who reported abortions were less likely to be strict as regards religious observance and less likely to have had a previous stillbirth or child death, other variables being equal. They were more likely to be smokers or former smokers and to be delivered of their babies in certain obstetric units. They more often reported vomiting, bleeding, and medication in early pregnancy. On the other hand, there was no significant association with diabetes, anaemia, blood groups, or season of birth.The findings show that women reporting previous induced abortions differ significantly from other pregnant women in a wide range of demographic and health characteristics. Such women may also be biased for complications of pregnancy and outcome, particularly if selected from a clinic population. Observations that indicate a deleterious effect of induced abortions on subsequent pregnancy outcomes must therefore be interpreted with considerable caution.", "contents": "Characteristics of pregnant women who report previous induced abortions. Associations between previous induced abortion and demographic and health factors in pregnancy were measured in 9 874 women who gave birth and who had been interviewed during pregnancy. Previous abortion was most rare among women having their first baby and increased with increasing birth order up to the fourth, thereafter decreasing. It was positively correlated with maternal age and negatively with age at marriage. There was no effect of years of schooling, when other variables were taken into account, but there were significant differences between ethnic groups, abortion being commonest among Jewish women from North African countries and more prevalent in those from western and Asian countries than in the second-generation Israel-born or in Arab women.Women who reported abortions were less likely to be strict as regards religious observance and less likely to have had a previous stillbirth or child death, other variables being equal. They were more likely to be smokers or former smokers and to be delivered of their babies in certain obstetric units. They more often reported vomiting, bleeding, and medication in early pregnancy. On the other hand, there was no significant association with diabetes, anaemia, blood groups, or season of birth.The findings show that women reporting previous induced abortions differ significantly from other pregnant women in a wide range of demographic and health characteristics. Such women may also be biased for complications of pregnancy and outcome, particularly if selected from a clinic population. Observations that indicate a deleterious effect of induced abortions on subsequent pregnancy outcomes must therefore be interpreted with considerable caution."}
{"id": "PMID:1083305", "title": "The relationship of race, sex, and age to concentrations of serum immunoglobulins expressed in international units in healthy adults in the USA.", "content": "Only a few investigations have been made to obtain human serum immunoglobulin values in units compatible with those used by the WHO International Reference Preparation for the Human Immunoglobulins IgG, IgA, and IgM. We report our summary statistics of serum IgG, IgA, and IgM, in international units (IU), for some 800 healthy American adults grouped by age, sex, and race. Our findings are in general agreement with some, but not with all, published data. We found that the mean IgG concentration is markedly higher and the mean IgA concentration is slightly higher in blacks than in whites. Except for white females, there was a significant increase in mean IgA with age for both races. In the younger adults of both races, mean IgM values were markedly higher in females than in males. Statistically significant interactions between race, age, and sex factors were seen for all three immunoglobulin classes. Although we have attempted to estimate the normal population means and variances for the serum concentration of IgG, IgA, and IgM the process we used to select specimens may have resulted in some bias; much larger, truly randomized, and fully documented studies in different geographic areas and in different socioeconomic and racial groups are needed to provide accurate acceptable limits for human immunoglobulins.", "contents": "The relationship of race, sex, and age to concentrations of serum immunoglobulins expressed in international units in healthy adults in the USA. Only a few investigations have been made to obtain human serum immunoglobulin values in units compatible with those used by the WHO International Reference Preparation for the Human Immunoglobulins IgG, IgA, and IgM. We report our summary statistics of serum IgG, IgA, and IgM, in international units (IU), for some 800 healthy American adults grouped by age, sex, and race. Our findings are in general agreement with some, but not with all, published data. We found that the mean IgG concentration is markedly higher and the mean IgA concentration is slightly higher in blacks than in whites. Except for white females, there was a significant increase in mean IgA with age for both races. In the younger adults of both races, mean IgM values were markedly higher in females than in males. Statistically significant interactions between race, age, and sex factors were seen for all three immunoglobulin classes. Although we have attempted to estimate the normal population means and variances for the serum concentration of IgG, IgA, and IgM the process we used to select specimens may have resulted in some bias; much larger, truly randomized, and fully documented studies in different geographic areas and in different socioeconomic and racial groups are needed to provide accurate acceptable limits for human immunoglobulins."}
{"id": "PMID:1083306", "title": "Specificity of the 48-hour reaction to Mitsuda antigen. Use of a soluble antigen from human and armadillo lepromin.", "content": "Two antigens were tested and compared in relation to the 48-h Fernandez reaction. They were obtained from standard human and from standard armadillo lepromin. All the tests were negative in patients with lepromatous leprosy and highly positive in those with tuberculoid leprosy and in lepromin-positive contacts. There was total agreement in all tests done with the two types of antigen. The antigenic component has the following basic properties: it precipitates with 80% saturated ammonium sulfate; it is not destroyed by autoclaving or by treatment with 0.4% phenol; it is non-dialysable; and it is destroyed by treatment with trypsin.", "contents": "Specificity of the 48-hour reaction to Mitsuda antigen. Use of a soluble antigen from human and armadillo lepromin. Two antigens were tested and compared in relation to the 48-h Fernandez reaction. They were obtained from standard human and from standard armadillo lepromin. All the tests were negative in patients with lepromatous leprosy and highly positive in those with tuberculoid leprosy and in lepromin-positive contacts. There was total agreement in all tests done with the two types of antigen. The antigenic component has the following basic properties: it precipitates with 80% saturated ammonium sulfate; it is not destroyed by autoclaving or by treatment with 0.4% phenol; it is non-dialysable; and it is destroyed by treatment with trypsin."}
{"id": "PMID:1083307", "title": "Tests with three antigens in leprosy-endemic and non-endemic areas.", "content": "A study comparing the 48-h and 30-day reactions produced by three antigens was made in areas of low and high leprosy endemicity in Venezuela and in areas of Chile, a non-endemic country. The antigens used for the intradermal tests were standard Mitsuda antigen, supernatant from standard Mitsuda antigen, and PPD. The results indicate that there is no difference in the Mitsuda reaction of persons living in areas of high or low endemicity, but they show a statistically significant difference between the reactions in persons who live in endemic areas and those of persons living in a country where the disease has not been described. The difference in the Fernandez reaction obtained with the supernatant was not statistically significant between the two population groups in the endemic country, but was highly significant when comparing the endemic and the non-endemic countries.", "contents": "Tests with three antigens in leprosy-endemic and non-endemic areas. A study comparing the 48-h and 30-day reactions produced by three antigens was made in areas of low and high leprosy endemicity in Venezuela and in areas of Chile, a non-endemic country. The antigens used for the intradermal tests were standard Mitsuda antigen, supernatant from standard Mitsuda antigen, and PPD. The results indicate that there is no difference in the Mitsuda reaction of persons living in areas of high or low endemicity, but they show a statistically significant difference between the reactions in persons who live in endemic areas and those of persons living in a country where the disease has not been described. The difference in the Fernandez reaction obtained with the supernatant was not statistically significant between the two population groups in the endemic country, but was highly significant when comparing the endemic and the non-endemic countries."}
{"id": "PMID:1083308", "title": "Immunopathology of nephropathies associated with malaria.", "content": "Immune complexes play an important role in the pathogenesis of malaria-associated nephropathies. Two main types of lesion are demonstrable: (a) acute (transient-reversible) lesions typical of falciparum infections in man, with mild clinical symptoms developing a week or two after infection. Renal biopsies at that time show deposits of immunoglobulins, complement, and sometimes antigen. The lesions respond to antimalarials. (b) Chronic (progressive) lesions characteristic of quartan infections in man, developing slowly into a chronic stage with persistent proteinuria and gradually deteriorating renal function and hypertension. Renal biopsies at the onset of the disease show deposits of immunoglobulins, complement, and P. malariae antigens in glomerular capillary walls. Antimalarial therapy has no effect. Recent immunochemical findings confirm that these lesions are of the immune-complex type and are associated with malaria infection. However, several questions remain to be solved.", "contents": "Immunopathology of nephropathies associated with malaria. Immune complexes play an important role in the pathogenesis of malaria-associated nephropathies. Two main types of lesion are demonstrable: (a) acute (transient-reversible) lesions typical of falciparum infections in man, with mild clinical symptoms developing a week or two after infection. Renal biopsies at that time show deposits of immunoglobulins, complement, and sometimes antigen. The lesions respond to antimalarials. (b) Chronic (progressive) lesions characteristic of quartan infections in man, developing slowly into a chronic stage with persistent proteinuria and gradually deteriorating renal function and hypertension. Renal biopsies at the onset of the disease show deposits of immunoglobulins, complement, and P. malariae antigens in glomerular capillary walls. Antimalarial therapy has no effect. Recent immunochemical findings confirm that these lesions are of the immune-complex type and are associated with malaria infection. However, several questions remain to be solved."}
{"id": "PMID:1083310", "title": "Determination of influenzavirus neuraminidase inhibition titres.", "content": "Representatives of the WHO influenza programme recently proposed a standard method of determining neuraminidase activity and neuraminidase inhibition (NI) antibody titres. Logit transformation of the data obtained with the WHO method for the NI assay permits a more efficient performance of the test and easy calculation of titres with a computer programme.", "contents": "Determination of influenzavirus neuraminidase inhibition titres. Representatives of the WHO influenza programme recently proposed a standard method of determining neuraminidase activity and neuraminidase inhibition (NI) antibody titres. Logit transformation of the data obtained with the WHO method for the NI assay permits a more efficient performance of the test and easy calculation of titres with a computer programme."}
{"id": "PMID:1083311", "title": "The penetration of three simuliid species by the nematode Reesimermis nielseni.", "content": "After 4 h of exposure to the preparasitic stage of R. nielseni first, second, and third instar Simulium venustum, S. decorum and S. vittatum were found to be harbouring the nematodes in the haemocoel and/or the gut. The possible use of R. nielseni in the biological control of simuliid species is discussed.", "contents": "The penetration of three simuliid species by the nematode Reesimermis nielseni. After 4 h of exposure to the preparasitic stage of R. nielseni first, second, and third instar Simulium venustum, S. decorum and S. vittatum were found to be harbouring the nematodes in the haemocoel and/or the gut. The possible use of R. nielseni in the biological control of simuliid species is discussed."}
{"id": "PMID:1083313", "title": "Prophylactic digitalization for coronary artery bypass surgery.", "content": "One hundred and twenty patients undergoing aortocoronary bypass procedures were randomly placed into control and digitalized groups. All were initially in normal sinus rhythm and without evidence of congestive heart failure. Supraventricular arrhythmias occurred in 17 of 66 controls and in only three of 54 digitalized patients (P less than 0.01). There was no evidence of digitals toxicity. Based on this evidence we recommend prophylactic digitalization for patients having aortocoronary bypass operations.", "contents": "Prophylactic digitalization for coronary artery bypass surgery. One hundred and twenty patients undergoing aortocoronary bypass procedures were randomly placed into control and digitalized groups. All were initially in normal sinus rhythm and without evidence of congestive heart failure. Supraventricular arrhythmias occurred in 17 of 66 controls and in only three of 54 digitalized patients (P less than 0.01). There was no evidence of digitals toxicity. Based on this evidence we recommend prophylactic digitalization for patients having aortocoronary bypass operations."}
{"id": "PMID:1083314", "title": "Atherosclerotic narrowing of the left main coronary artery. A necropsy analysis of 152 patients with fatal coronary heart disease and varying degrees of left main narrowing.", "content": "Histologic sections of the left main (LM) and the other three major coronary arteries were studied in 152 patients. The lumen of the LM in 35 patients was greater than 75% narrowed; in thirty, 50-75%; and in 87, less than 50% narrowed. The patients with greater than 75% narrowing were younger. Angina pectoris and hyperlipoproteinemia, specifically type II, were more common (P less than 0.02) and acute transmural and healed subendocardial myocardial infarcts were less frequent (P less than 0.05) in the patients with greater 75% LM narrowing than in those with less than 50% narrowing. Of the three other major coronary arteries, the average number narrowed in the patients with greater than 75% LM narrowing was 2.9; in those with 50-75% LM narrowing 2.7, and in those with less than 50% LM narrowing, 2.4. Of the 35 patients with greater than 75% LM narrowing, 33 had greater than 75% luminal narrowing of each of the other three major coronary arteries. Narrowing of the LM, therefore, indicates severe narrowing of usually all major coronary arteries.", "contents": "Atherosclerotic narrowing of the left main coronary artery. A necropsy analysis of 152 patients with fatal coronary heart disease and varying degrees of left main narrowing. Histologic sections of the left main (LM) and the other three major coronary arteries were studied in 152 patients. The lumen of the LM in 35 patients was greater than 75% narrowed; in thirty, 50-75%; and in 87, less than 50% narrowed. The patients with greater than 75% narrowing were younger. Angina pectoris and hyperlipoproteinemia, specifically type II, were more common (P less than 0.02) and acute transmural and healed subendocardial myocardial infarcts were less frequent (P less than 0.05) in the patients with greater 75% LM narrowing than in those with less than 50% narrowing. Of the three other major coronary arteries, the average number narrowed in the patients with greater than 75% LM narrowing was 2.9; in those with 50-75% LM narrowing 2.7, and in those with less than 50% LM narrowing, 2.4. Of the 35 patients with greater than 75% LM narrowing, 33 had greater than 75% luminal narrowing of each of the other three major coronary arteries. Narrowing of the LM, therefore, indicates severe narrowing of usually all major coronary arteries."}
{"id": "PMID:1083315", "title": "Profile analysis of blood proteins with a centrifugal analyzer.", "content": "We describe a procedure for routine analysis of a profile composed of eight blood proteins with a GEMSAEC centrifugal analyzer. The end-point technique is used and four standards are included in each run in the immunochemical analysis of the individual proteins. Additional computer programs fit the absorbance readings from the samples to the (nonlinear) standard curve and store the results in a patient's file for a final printout, which also presents the patient's data and reference values. The proteins are analyzed in a given order, which allows common blank runs to be used. Antisera are used in suitably high concentrations, so that the \"antigen in excess\" problem only appears for analysis of IgA and IgM when sera contain high amounts of the M-components of these two classes. The capacity, accuracy, and precision of the method are satisfactory.", "contents": "Profile analysis of blood proteins with a centrifugal analyzer. We describe a procedure for routine analysis of a profile composed of eight blood proteins with a GEMSAEC centrifugal analyzer. The end-point technique is used and four standards are included in each run in the immunochemical analysis of the individual proteins. Additional computer programs fit the absorbance readings from the samples to the (nonlinear) standard curve and store the results in a patient's file for a final printout, which also presents the patient's data and reference values. The proteins are analyzed in a given order, which allows common blank runs to be used. Antisera are used in suitably high concentrations, so that the \"antigen in excess\" problem only appears for analysis of IgA and IgM when sera contain high amounts of the M-components of these two classes. The capacity, accuracy, and precision of the method are satisfactory."}
{"id": "PMID:1083312", "title": "Vision screening in a national sample of 11-year-old children.", "content": "This report describes the results of vision screening carried out by local health authorities on a national sample of 11-year-old schoolchildren using a standard Snellen chart. Of the 12 772 children tested, 78% had an unaided distant visual acuity of 6/6 or better in both eyes (optimal vision), 10% had a distant visual acuity of 6/9 in the worse or both eyes (near-optimal vision) and 12% had a visual acuity of 6/12 or worse in one or both eyes eyes (definite visual defect). In addition, near visual acuity was tested for 12 737 children and 5% were found to have defective near vision. Glasses had been prescribed for current use in 12% of children but a quarter of those prescribed glasses did not have them available at the time of the test. Testing revealed that 22% of children whose glasses were available had optimal or near-optimal unaided distant vision, the number increasing to 98% when retested wearing glasses. In contrast, 43% of the children who were without their glasses had optimal or near-optimal vision; 27% had a bilateral defect. Amongst the children for whom glasses had not been prescribed 4-6% had a visual defect. A higher proportion of children from non-manual family background than from manual family background had visual impairment and had been prescribed glasses, but there was no significant social class difference amongst the children with visual defects for whom no glasses had been prescribed. A defect of red/green colour vision was recorded in 6% of boys and 1% of girls. The proportion of children with poor visual acuity was similar in the group of children with defective colour vision and the group with normal colour vision.", "contents": "Vision screening in a national sample of 11-year-old children. This report describes the results of vision screening carried out by local health authorities on a national sample of 11-year-old schoolchildren using a standard Snellen chart. Of the 12 772 children tested, 78% had an unaided distant visual acuity of 6/6 or better in both eyes (optimal vision), 10% had a distant visual acuity of 6/9 in the worse or both eyes (near-optimal vision) and 12% had a visual acuity of 6/12 or worse in one or both eyes eyes (definite visual defect). In addition, near visual acuity was tested for 12 737 children and 5% were found to have defective near vision. Glasses had been prescribed for current use in 12% of children but a quarter of those prescribed glasses did not have them available at the time of the test. Testing revealed that 22% of children whose glasses were available had optimal or near-optimal unaided distant vision, the number increasing to 98% when retested wearing glasses. In contrast, 43% of the children who were without their glasses had optimal or near-optimal vision; 27% had a bilateral defect. Amongst the children for whom glasses had not been prescribed 4-6% had a visual defect. A higher proportion of children from non-manual family background than from manual family background had visual impairment and had been prescribed glasses, but there was no significant social class difference amongst the children with visual defects for whom no glasses had been prescribed. A defect of red/green colour vision was recorded in 6% of boys and 1% of girls. The proportion of children with poor visual acuity was similar in the group of children with defective colour vision and the group with normal colour vision."}
{"id": "PMID:1083316", "title": "Characterization of mononuclear effector cells in human blood.", "content": "The effector cells responsible for cytotoxic activity induced by phytohaemagglutinin, (PHA) pokeweed mitogen (PWM) target cells complexes with IgG antibody has been investigated using cell separation techniques based on rosette formation and separation through Hypaque-Ficoll mixtures. It was shown the PHA-induced cytotoxicity is predominantly a function of T cells and that Fc receptor-bearing cells are not involved to any major extent. Antibody-dependent killing is conversely a function of Fc receptor-bearing cells among which two subtypes can be distinguished. One of these has receptors for activated complement while the other bears Fc receptors only and has no detectable receptors for complement. PWM appears to induce cytotoxicity in both T- and non-T-cell populations but the major cell type involved appears to be Fc receptor-bearing cells similar to those mediating antibody-dependent killing. It is concluded that PHA and antibody-dependent killing are the two most useful assays for discriminating between the cytotoxic activity of T and non-T cell in clinical studies.", "contents": "Characterization of mononuclear effector cells in human blood. The effector cells responsible for cytotoxic activity induced by phytohaemagglutinin, (PHA) pokeweed mitogen (PWM) target cells complexes with IgG antibody has been investigated using cell separation techniques based on rosette formation and separation through Hypaque-Ficoll mixtures. It was shown the PHA-induced cytotoxicity is predominantly a function of T cells and that Fc receptor-bearing cells are not involved to any major extent. Antibody-dependent killing is conversely a function of Fc receptor-bearing cells among which two subtypes can be distinguished. One of these has receptors for activated complement while the other bears Fc receptors only and has no detectable receptors for complement. PWM appears to induce cytotoxicity in both T- and non-T-cell populations but the major cell type involved appears to be Fc receptor-bearing cells similar to those mediating antibody-dependent killing. It is concluded that PHA and antibody-dependent killing are the two most useful assays for discriminating between the cytotoxic activity of T and non-T cell in clinical studies."}
{"id": "PMID:1083317", "title": "Lymphocyte populations in peripheral blood in hyperthyroid and euthyroid subjects.", "content": "Thymus-derived and non-thymus-derived peripheral blood lymphocytes were examined by means of E- and EAC-rosette tests. The frequency of these lymphocyte populations was the same in hyperthyroid and euthyroid individuals. It was also demonstrated that PHA responsiveness of lymphocytes from hyperthyroid patients was equal to that of lymphocytes from euthyroid controls and did not change after treatment with 131I.", "contents": "Lymphocyte populations in peripheral blood in hyperthyroid and euthyroid subjects. Thymus-derived and non-thymus-derived peripheral blood lymphocytes were examined by means of E- and EAC-rosette tests. The frequency of these lymphocyte populations was the same in hyperthyroid and euthyroid individuals. It was also demonstrated that PHA responsiveness of lymphocytes from hyperthyroid patients was equal to that of lymphocytes from euthyroid controls and did not change after treatment with 131I."}
{"id": "PMID:1083318", "title": "B and T lymphocytes in Hodgkin's disease. Analysis at diagnosis and following therapy.", "content": "B and T lymphocytes were studied in the blood and lymph nodes of fifty patients with Hodgkin's disease. At diagnosis, most patients (77%) had normal percentages of circulating B and T lymphocytes. Most patients (60%) also showed normal percentages of B and T lymphocytes in involved lymph nodes. Splenectomy had no effect on circulating B and T lymphocytes in four of five patients studied 2 weeks postoperatively. Seventeen patients were studied before and after treatment to determine the effect of therapy. Very high percentages of B lymphocytes were found in patients studied following radiotherapy. It appears that the known defects in cell-mediated immunity in Hodgkin's disease are not expressed as significant abnormalities in B-or T-lymphocyte ratios in blood or lymphoid tissues at diagnosis. The proliferation of B lymphocytes following radiotherapy could represent a compensatory mechanism to cell-mediated deficiency or a loss of normal suppressor activity of T lymphocytes.", "contents": "B and T lymphocytes in Hodgkin's disease. Analysis at diagnosis and following therapy. B and T lymphocytes were studied in the blood and lymph nodes of fifty patients with Hodgkin's disease. At diagnosis, most patients (77%) had normal percentages of circulating B and T lymphocytes. Most patients (60%) also showed normal percentages of B and T lymphocytes in involved lymph nodes. Splenectomy had no effect on circulating B and T lymphocytes in four of five patients studied 2 weeks postoperatively. Seventeen patients were studied before and after treatment to determine the effect of therapy. Very high percentages of B lymphocytes were found in patients studied following radiotherapy. It appears that the known defects in cell-mediated immunity in Hodgkin's disease are not expressed as significant abnormalities in B-or T-lymphocyte ratios in blood or lymphoid tissues at diagnosis. The proliferation of B lymphocytes following radiotherapy could represent a compensatory mechanism to cell-mediated deficiency or a loss of normal suppressor activity of T lymphocytes."}
{"id": "PMID:1083319", "title": "Morphological, immunological and immunocytochemical identification of lymphocytes extracted from cutaneous infiltrates.", "content": "The identification of mononuclear cells extracted from various skin disorders has been investigated and the relative amount of B and T cells determined according to various assays such as E-rosette test, FITC and peroxidase anti-immunoglobulin conjugates. It has been found that: (a) there were two different patterns of distribution of the cells in the infiltrates; one with a T/B ratio above 3 (closest to a delayed type reaction), the other with a T/B ratio below 2. (b) The morphological appearance of tissue lymphocytes differs from that of circulating lymphocytes by the presence of both membrane and cytoplasmic immunoglobulins.", "contents": "Morphological, immunological and immunocytochemical identification of lymphocytes extracted from cutaneous infiltrates. The identification of mononuclear cells extracted from various skin disorders has been investigated and the relative amount of B and T cells determined according to various assays such as E-rosette test, FITC and peroxidase anti-immunoglobulin conjugates. It has been found that: (a) there were two different patterns of distribution of the cells in the infiltrates; one with a T/B ratio above 3 (closest to a delayed type reaction), the other with a T/B ratio below 2. (b) The morphological appearance of tissue lymphocytes differs from that of circulating lymphocytes by the presence of both membrane and cytoplasmic immunoglobulins."}
{"id": "PMID:1083325", "title": "Report of five children with Hemophilus influenzae pneumonia. Resistance to ampicillin must always be looked for.", "content": "Five cases of Hemophilus influenzae pneumonia were observed in a 13-month period at Fairfax Hospital, Fairfax, Va. More aggressive diagnostic procedures may be required in order to diagnose this disease with greater frequency. This has become especially important in view of the growing recovery of ampicillin-resistant H. influenzae strains.", "contents": "Report of five children with Hemophilus influenzae pneumonia. Resistance to ampicillin must always be looked for. Five cases of Hemophilus influenzae pneumonia were observed in a 13-month period at Fairfax Hospital, Fairfax, Va. More aggressive diagnostic procedures may be required in order to diagnose this disease with greater frequency. This has become especially important in view of the growing recovery of ampicillin-resistant H. influenzae strains."}
{"id": "PMID:1083326", "title": "Marijuana and T lymphocyte rosettes.", "content": "The effect of marijuana smoking on sheep cell rosetting properties of both early (active) and total T lymphocytes was studied in vitro. Significantly fewer active rosettes were formed by T cells from a population of 35 who appeared to be chronic marijuana smokers than the 34 controls. The late, or cold-enhanced rosettes formed by smokers and nonsmokers were similar, suggesting that similar numbers of rosette-forming T cells were present in the peripheral blood of smokers. These data suggest that marijuana smoking may affect the function of a subpopulation of T lymphocytes, identified by their capacity to form early rosettes, possibly by affecting these cell membranes.", "contents": "Marijuana and T lymphocyte rosettes. The effect of marijuana smoking on sheep cell rosetting properties of both early (active) and total T lymphocytes was studied in vitro. Significantly fewer active rosettes were formed by T cells from a population of 35 who appeared to be chronic marijuana smokers than the 34 controls. The late, or cold-enhanced rosettes formed by smokers and nonsmokers were similar, suggesting that similar numbers of rosette-forming T cells were present in the peripheral blood of smokers. These data suggest that marijuana smoking may affect the function of a subpopulation of T lymphocytes, identified by their capacity to form early rosettes, possibly by affecting these cell membranes."}
{"id": "PMID:1083323", "title": "Percutaneous electrical stimulation for clinical tibial fracture repair.", "content": "Observations on stress generated electrical currents in bone have stimulated interest in the possible osteogenetic effect of externally applied electrical energy to establish diaphyseal bone defects in animals and man. This report records the observed effects of pulsed electrical energy directed through a metal (Riordan pin) electrode placed in a large proximal tibial shaft dedect in a young man who sustained loss of bone from a gunshot wound. The fixation pin placed directly into the defect provided the cathode (-) electrode. The anode (+) electrode consisted of an aluminum foil band placed on the skin adjacent to the leg. The tibia had 282 consecutive days of electrical stimulation and provided X-ray and clinical evidence of enhanced osteogenetic activity. The degree of osteogenetic response attributable to the electrical stimulation is undetermined because other factors, including cast immobilization, time and minimal touchdown (25 pounds) weight-bearing in the cast during the period of observation, may also have had some influence on the healing response. Circumstantial clinical evidence indicates that the applied electrical energy was of primary importance in the healing process. This theoretically and technically acceptable source of osteogenetic activity merits continued, intensive investigation.", "contents": "Percutaneous electrical stimulation for clinical tibial fracture repair. Observations on stress generated electrical currents in bone have stimulated interest in the possible osteogenetic effect of externally applied electrical energy to establish diaphyseal bone defects in animals and man. This report records the observed effects of pulsed electrical energy directed through a metal (Riordan pin) electrode placed in a large proximal tibial shaft dedect in a young man who sustained loss of bone from a gunshot wound. The fixation pin placed directly into the defect provided the cathode (-) electrode. The anode (+) electrode consisted of an aluminum foil band placed on the skin adjacent to the leg. The tibia had 282 consecutive days of electrical stimulation and provided X-ray and clinical evidence of enhanced osteogenetic activity. The degree of osteogenetic response attributable to the electrical stimulation is undetermined because other factors, including cast immobilization, time and minimal touchdown (25 pounds) weight-bearing in the cast during the period of observation, may also have had some influence on the healing response. Circumstantial clinical evidence indicates that the applied electrical energy was of primary importance in the healing process. This theoretically and technically acceptable source of osteogenetic activity merits continued, intensive investigation."}
{"id": "PMID:1083324", "title": "Alteration in the force and fatigability of skeletal muscle in quadriplegic humans following exercise induced by chronic electrical stimulation.", "content": "The paralyzed forearm finger flexor muscles of 10 quadriplegic subjects were exercised via electrical stimulation to determine changes in the contractile force and fatigability of the muscle that could be externally induced. The force of contraction initially produced by most muscles was less than that required for functional activity. Following exercise, the strength of all muscles was increased above the level for functional use. In general, no correlation was noted between the amount of exercise and the force change. Two subjects had high contraction forces after prolonged periods before stimulation began, and 4 subjects who were followed after the stimulation program maintained near the contractile force achieved at the end of the program in the absence of stimulation. Fatigue of the muscle during the course of the exercise program was increased to above that recorded initially, although this was more variable and sometimes dropped to less than that recorded initially. Generally, no correlation was demonstrated between usage and fatigue. It is concluded that exercise induced by electrical stimulation alters the cantractile properties of the muscle toward a state usable for functional, tonic activation.", "contents": "Alteration in the force and fatigability of skeletal muscle in quadriplegic humans following exercise induced by chronic electrical stimulation. The paralyzed forearm finger flexor muscles of 10 quadriplegic subjects were exercised via electrical stimulation to determine changes in the contractile force and fatigability of the muscle that could be externally induced. The force of contraction initially produced by most muscles was less than that required for functional activity. Following exercise, the strength of all muscles was increased above the level for functional use. In general, no correlation was noted between the amount of exercise and the force change. Two subjects had high contraction forces after prolonged periods before stimulation began, and 4 subjects who were followed after the stimulation program maintained near the contractile force achieved at the end of the program in the absence of stimulation. Fatigue of the muscle during the course of the exercise program was increased to above that recorded initially, although this was more variable and sometimes dropped to less than that recorded initially. Generally, no correlation was demonstrated between usage and fatigue. It is concluded that exercise induced by electrical stimulation alters the cantractile properties of the muscle toward a state usable for functional, tonic activation."}
{"id": "PMID:1083329", "title": "Massive gastrointestinal hemorrhage.", "content": "Dissatisfaction with the high morbidity and mortality of traditional methods of handling massive gastrointestinal hemorrhage has led to the exploration of means other than surgical to attain hemostasis. Some, such as selective arterial infusion of surgical Pituitrin, have quickly won general acceptance in hospitals where facilities and interested personnel are available. Others, such as alkalinization, have become popular because of their inherent simplicity. Systemic hypothermia, requiring intensive patient care, has not been without considerable risk of significant complications. Iced saline lavage has never been subjected to critical evaluation. It is possible that the emptying of the stomach through mechanical destruction of the intragastric clot by repeated irrigations, reducing the antral stimulation by relief of distension, may be as important as the temperature of the solution in the stomach. Gastric irrigations with norepinephrine solutions have awaited the results of physiologic studies showing that the cardiovascular and renal effects of injected levarterenol are avoided, and that permanent damage to the gastrointestinal mucosa does not result. Trials have been confined largely to very poor-risk patients, and the hemostasis that has resulted has not been explainable, in all cases, on the basis of the physiologic activity of the agent (e.g., control of bleeding from tumor vessels). Evacuation of gastric content prior to introduction of the norepinephrine solution seems important. Lower gastrointestinal bleeding from benign disease has also responded to advances in applied pharmacology, with intra-arterial infusion of surgical Pituitrin again coming into progressively wider use. Intraperitoneal instillation of norepinephrine has also proved useful, even in patients who have adhesions from prior surgery or inflammatory disease, but closer monitoring of blood pressure and urine output are necessary because some of this solution is absorbed by the parietal peritoneum and not deactivated by the liver before entering the systemic circulation. Taken together, selective arterial infusion of vasopressin and topical application of norepinephrine can be considered complementary rather than competitive therapies. Because of the more extensive experience with selective angiographic infusion, it should be the first choice in institutions where it is readily available. For patients in whom arterial puncture is inadvisable, and where angiography is not readily available, topically applied norepinephrine becomes the treatment of preference. We have demonstrated effectiveness of intraperitoneal norepinephrine in a patient in whom selective arterial infusion of surgical Pituitrin had failed. And the reverse would probably also hold true on occasion. Pharmacologic techniques represent a therapeutic advance, reducing the frequency with which surgical intervention becomes mandatory. But they are not a substitute for surgery...", "contents": "Massive gastrointestinal hemorrhage. Dissatisfaction with the high morbidity and mortality of traditional methods of handling massive gastrointestinal hemorrhage has led to the exploration of means other than surgical to attain hemostasis. Some, such as selective arterial infusion of surgical Pituitrin, have quickly won general acceptance in hospitals where facilities and interested personnel are available. Others, such as alkalinization, have become popular because of their inherent simplicity. Systemic hypothermia, requiring intensive patient care, has not been without considerable risk of significant complications. Iced saline lavage has never been subjected to critical evaluation. It is possible that the emptying of the stomach through mechanical destruction of the intragastric clot by repeated irrigations, reducing the antral stimulation by relief of distension, may be as important as the temperature of the solution in the stomach. Gastric irrigations with norepinephrine solutions have awaited the results of physiologic studies showing that the cardiovascular and renal effects of injected levarterenol are avoided, and that permanent damage to the gastrointestinal mucosa does not result. Trials have been confined largely to very poor-risk patients, and the hemostasis that has resulted has not been explainable, in all cases, on the basis of the physiologic activity of the agent (e.g., control of bleeding from tumor vessels). Evacuation of gastric content prior to introduction of the norepinephrine solution seems important. Lower gastrointestinal bleeding from benign disease has also responded to advances in applied pharmacology, with intra-arterial infusion of surgical Pituitrin again coming into progressively wider use. Intraperitoneal instillation of norepinephrine has also proved useful, even in patients who have adhesions from prior surgery or inflammatory disease, but closer monitoring of blood pressure and urine output are necessary because some of this solution is absorbed by the parietal peritoneum and not deactivated by the liver before entering the systemic circulation. Taken together, selective arterial infusion of vasopressin and topical application of norepinephrine can be considered complementary rather than competitive therapies. Because of the more extensive experience with selective angiographic infusion, it should be the first choice in institutions where it is readily available. For patients in whom arterial puncture is inadvisable, and where angiography is not readily available, topically applied norepinephrine becomes the treatment of preference. We have demonstrated effectiveness of intraperitoneal norepinephrine in a patient in whom selective arterial infusion of surgical Pituitrin had failed. And the reverse would probably also hold true on occasion. Pharmacologic techniques represent a therapeutic advance, reducing the frequency with which surgical intervention becomes mandatory. But they are not a substitute for surgery..."}
{"id": "PMID:1083331", "title": "Gastro-intestinal bleeding in patients with rheumatoid arthritis: the effects of azapropazone treatment.", "content": "A comparative study was carried out, using the Cr51 red blood cell labelling method, to assess the amount of gastro-intestinal blood loss in 20 rheumatoid arthritis patients taking either 600 mg. or 1200 mg. azapropazone daily. The results demonstrate that azapropazone does not produce greater than normal gastro-intestinal bleeding, that the volume of faecal blood loss is not dose-related, and there would not appear to be any correlation between blood loss and the occasional mild gastro-intestinal side-effects reported in a few patients. These results compared favourably with those obtained previously by the investigators in a similar group of patients taking 3 g. aspirin per day.", "contents": "Gastro-intestinal bleeding in patients with rheumatoid arthritis: the effects of azapropazone treatment. A comparative study was carried out, using the Cr51 red blood cell labelling method, to assess the amount of gastro-intestinal blood loss in 20 rheumatoid arthritis patients taking either 600 mg. or 1200 mg. azapropazone daily. The results demonstrate that azapropazone does not produce greater than normal gastro-intestinal bleeding, that the volume of faecal blood loss is not dose-related, and there would not appear to be any correlation between blood loss and the occasional mild gastro-intestinal side-effects reported in a few patients. These results compared favourably with those obtained previously by the investigators in a similar group of patients taking 3 g. aspirin per day."}
{"id": "PMID:1083332", "title": "[Cytotoxic effect of antihuman thymocyte serum on T lymphocytes].", "content": "The rabbit human thymocyte serum had a selective cytotoxicity on human T lymphocytes.  In the presence of guinea pig complement, 23.8% of the otal blood leukocytes were killed by the heterologus antiserum and only 4.2 % were killed by normal rabbit serum.  On average 28.2% of the leukocyte preparation obtained by dextran sedmentation were lymphocytes.  According to the above figures, 72.3% of the circulating lymphocytes were identified as T cells.  The data were comparable to those obtained from other conventional methods used in laboratory animals.  The cytotoxicity test was found to be a reliable technique for identifying human T lymphocytes.", "contents": "[Cytotoxic effect of antihuman thymocyte serum on T lymphocytes]. The rabbit human thymocyte serum had a selective cytotoxicity on human T lymphocytes.  In the presence of guinea pig complement, 23.8% of the otal blood leukocytes were killed by the heterologus antiserum and only 4.2 % were killed by normal rabbit serum.  On average 28.2% of the leukocyte preparation obtained by dextran sedmentation were lymphocytes.  According to the above figures, 72.3% of the circulating lymphocytes were identified as T cells.  The data were comparable to those obtained from other conventional methods used in laboratory animals.  The cytotoxicity test was found to be a reliable technique for identifying human T lymphocytes."}
{"id": "PMID:1083335", "title": "[Haemobilia as a special form of gastro-intestinal bleeding (author's transl)].", "content": "Nine patients with haemobilia were treated during a 17-year period (1958-1974), seven of them with postoperative haemobilia after operations on the biliary tract, and one with traumatic haemobilia. Three patients with intrahepatic injury after transhepatic drainage for inflammatory biliary-tract stricture or after extraction of intrahepatic biliary-tract stones with a Fogarty catheter were free of symptoms on conservative treatment alone. Of six operated patients one died from extensive hepatic necrosis after ligation of the main and right hepatic arteries, another two months after operation of septicaemia. The other patients have remained free of symptoms.", "contents": "[Haemobilia as a special form of gastro-intestinal bleeding (author's transl)]. Nine patients with haemobilia were treated during a 17-year period (1958-1974), seven of them with postoperative haemobilia after operations on the biliary tract, and one with traumatic haemobilia. Three patients with intrahepatic injury after transhepatic drainage for inflammatory biliary-tract stricture or after extraction of intrahepatic biliary-tract stones with a Fogarty catheter were free of symptoms on conservative treatment alone. Of six operated patients one died from extensive hepatic necrosis after ligation of the main and right hepatic arteries, another two months after operation of septicaemia. The other patients have remained free of symptoms."}
{"id": "PMID:1083339", "title": "[Study of the effect of the enzyme hyaluronidase on the pathomorphological changes in certain internal organs in white mice infected intraperitoneally with Tr. vaginalis].", "content": "The authors injected daily a dose of 5 l hyaluronidase diluted in 0,5 ml of saline, in 25 out of 50 white mice, contaminated with tr. vaginalis intraperitonealy. The remaining 25 mice formed the control group and were injected only with saline. The administration was made intraperitonealy. An equal number of mice from both groups were killed on the 30th and 40th day of contamination and the macroscopic changes in the abdominal cavity were examined. Histological examinations of liver, spleen and kidneys were made as well. The obtained pathologic changes were characteristic of manifestations of chronice en pusforming inflammatory process, involving mostly liver. Substantially differences in character and gravity of the pathologic changes between mice treated with hyaluronidase and those of the control group were not found.", "contents": "[Study of the effect of the enzyme hyaluronidase on the pathomorphological changes in certain internal organs in white mice infected intraperitoneally with Tr. vaginalis]. The authors injected daily a dose of 5 l hyaluronidase diluted in 0,5 ml of saline, in 25 out of 50 white mice, contaminated with tr. vaginalis intraperitonealy. The remaining 25 mice formed the control group and were injected only with saline. The administration was made intraperitonealy. An equal number of mice from both groups were killed on the 30th and 40th day of contamination and the macroscopic changes in the abdominal cavity were examined. Histological examinations of liver, spleen and kidneys were made as well. The obtained pathologic changes were characteristic of manifestations of chronice en pusforming inflammatory process, involving mostly liver. Substantially differences in character and gravity of the pathologic changes between mice treated with hyaluronidase and those of the control group were not found."}
{"id": "PMID:1083342", "title": "Effects of cytochalasin B on the electrical activity of frog muscle fibers.", "content": "Cytochalasin B (50 mug/ml) markedly prolonged the falling phase of the action potential in frog sartorius muscle fibers without any effect on the rising phase and the amplitude of the action potential, nor on the resting potential. The maximum rate of fall of the action potential was reduced from 173 to 133 V/sec. The early depolarizing after-potential was increased in amplitude and lasted for as long as 1 sec. These changes in the falling phase of the action potential appear to be due to a slowing of sodium inactivation. Potassium conductance increase was not appreciably affected. The muscle treated with cytochalasin B responded to nerve stimulation with repetitive discharges which originated in presynaptic nerve elements.", "contents": "Effects of cytochalasin B on the electrical activity of frog muscle fibers. Cytochalasin B (50 mug/ml) markedly prolonged the falling phase of the action potential in frog sartorius muscle fibers without any effect on the rising phase and the amplitude of the action potential, nor on the resting potential. The maximum rate of fall of the action potential was reduced from 173 to 133 V/sec. The early depolarizing after-potential was increased in amplitude and lasted for as long as 1 sec. These changes in the falling phase of the action potential appear to be due to a slowing of sodium inactivation. Potassium conductance increase was not appreciably affected. The muscle treated with cytochalasin B responded to nerve stimulation with repetitive discharges which originated in presynaptic nerve elements."}
{"id": "PMID:1083349", "title": "[Unstable angina: observtions on 187 patients of whom 84 were operated on for aorto-coronary bypass (author's transl)].", "content": "Clinical data, EKG and coronary angiography of 187 patients with unstable angina are given. The patients are divided into 4 groups: spontancous angina, spontaneous angina Prinzmetal's variant, \"in crescendo\" agina, intermediate syndrome. 103 patients were treated with pharmacological therapy only and 84 underwent aortocoronary bypass; 2 of them were operated on for acute myocardial infarction. The clinical and pathologic peculiarities of two groups are not similar and therefore the results are not comparable. There was a mortality rate of 10.3% of patients with pharmacological treatment and the incidence of non fatal myocardial infarction was 10.4%. The mortality during operation was 14.2% and the incidence of non fatal myocardial infarction was 13.1%. The spontaneous angina and Prinzmetal's variant often had normal rest EKG and a very similar coronary angiographic pattern, with obstructive lesions often localized in the proximal part of a single vessel. In 9% of cases coronary arteries were normal. The incidence of serious arrhythmias was higher in Prinzmetal's variant than in other types of unstable angina. The mortality in patients with Prinzmetal's angina was particularly high during the first period of experience (1970-1973) when the operation was performed after a few days of unsatisfactory results with pharmacological treatment; it decreased after patients underwent operation in an attenuate phase of the disease. The rest EKG of \"in crescendo\" angina was almost always pathologic; these cases presented obstructive lesions of 2 or 3 coronary arteries, functional impairment of left ventricle, collateral circulation. The mortality in patients treated with pharmacological therapy was higher than in other types of unstable angina. The mortality during operation, fairly high in the first period of experience, decreased in this group of patients as well, during the second period of experience.", "contents": "[Unstable angina: observtions on 187 patients of whom 84 were operated on for aorto-coronary bypass (author's transl)]. Clinical data, EKG and coronary angiography of 187 patients with unstable angina are given. The patients are divided into 4 groups: spontancous angina, spontaneous angina Prinzmetal's variant, \"in crescendo\" agina, intermediate syndrome. 103 patients were treated with pharmacological therapy only and 84 underwent aortocoronary bypass; 2 of them were operated on for acute myocardial infarction. The clinical and pathologic peculiarities of two groups are not similar and therefore the results are not comparable. There was a mortality rate of 10.3% of patients with pharmacological treatment and the incidence of non fatal myocardial infarction was 10.4%. The mortality during operation was 14.2% and the incidence of non fatal myocardial infarction was 13.1%. The spontaneous angina and Prinzmetal's variant often had normal rest EKG and a very similar coronary angiographic pattern, with obstructive lesions often localized in the proximal part of a single vessel. In 9% of cases coronary arteries were normal. The incidence of serious arrhythmias was higher in Prinzmetal's variant than in other types of unstable angina. The mortality in patients with Prinzmetal's angina was particularly high during the first period of experience (1970-1973) when the operation was performed after a few days of unsatisfactory results with pharmacological treatment; it decreased after patients underwent operation in an attenuate phase of the disease. The rest EKG of \"in crescendo\" angina was almost always pathologic; these cases presented obstructive lesions of 2 or 3 coronary arteries, functional impairment of left ventricle, collateral circulation. The mortality in patients treated with pharmacological therapy was higher than in other types of unstable angina. The mortality during operation, fairly high in the first period of experience, decreased in this group of patients as well, during the second period of experience."}
{"id": "PMID:1083350", "title": "Symptomatic hepatic disease in cystic fibrosis: incidence, course, and outcome of portal systemic hunting.", "content": "Fifteen (2.2%) of 693 patients with cystic fibrosis seen over an 18-year period developed clinical hepatic disease. In 13 patients all symptoms were secondary to portal hypertension. Ten had hypersplenism and 6 had variceal bleeding, including 3 who developed both conditions. All 5 patients who survived the initial episode of gastrointestinal bleeding underwent portal systemic shunting. A shunting procedure also was performed on 1 patients with hypersplenism but no variceal bleeding. No subsequent deterioration of intellectual function occurred in either the shunted or unshunted patients. Only 1 of the shunted patients showed progression of hepatic disease after surgery. These results suggest that portal systemic shunting is useful in the treatment of bleeding esophageal varices in cystic fibrosis. A sweat test to rule out cystic fibrosis should be included in the evaluation of any teenage or young adult patient with unexplained portal hypertension.", "contents": "Symptomatic hepatic disease in cystic fibrosis: incidence, course, and outcome of portal systemic hunting. Fifteen (2.2%) of 693 patients with cystic fibrosis seen over an 18-year period developed clinical hepatic disease. In 13 patients all symptoms were secondary to portal hypertension. Ten had hypersplenism and 6 had variceal bleeding, including 3 who developed both conditions. All 5 patients who survived the initial episode of gastrointestinal bleeding underwent portal systemic shunting. A shunting procedure also was performed on 1 patients with hypersplenism but no variceal bleeding. No subsequent deterioration of intellectual function occurred in either the shunted or unshunted patients. Only 1 of the shunted patients showed progression of hepatic disease after surgery. These results suggest that portal systemic shunting is useful in the treatment of bleeding esophageal varices in cystic fibrosis. A sweat test to rule out cystic fibrosis should be included in the evaluation of any teenage or young adult patient with unexplained portal hypertension."}
{"id": "PMID:1083352", "title": "Relationship of splanchnic blood flow and portal venous resistance to elevated portal pressure in the dog.", "content": "To determine the relationship of splanchnic blood flow and portal venous resistance to elevated portal pressure, in situ perfusion of the splanchnic circuit was carried out in 12 freshly killed dogs at varying perfusion rates and degrees of acute and chronic portal vein constriction. In six normal dogs before being killed, portal pressure averaged 8-8 +/- 1-2 mmHg and portal flow 658 +/- 81 ml/min. In the absence of portal vein constriction, increasing splanchnic perfusion to 1800 ml/min, minimally raised portal pressure (12-8 +/- 1-5 mmHg). With progressive acute constriction of the portal vein, however, comparable perfusion rates lead to progressively higher portal pressure levels and with greater than 90% constriction, portal pressure was greater than 30 mmHg with minimal elevation in splanchnic flow rate. In six other dogs before being killed but live to nine weeks after placement of an ameroid constrictor on the portal vein, portal pressure averaged 13-6 +/- 1-4 mmHg or slightly higher than in normal dogs (P greater than 0-02). Mesenteric venography and necropsy findings uniformly demonstrated 90% occlusion of the portal vein with extensive portasystemic collateralization. With increased perfusion of the splanchnic bed, portal pressure rose rapidly to approximately 35 mmHg with a flow rate of 1 800 ml/min.. These data suggest that, in disorders where resistance to transhepatic portal flow is marked, a small increment in splanchnic blood flow, which normally exerts little or no influence on portal pressure, promotes profound portal hypertension and may account for spontaneous 'rupture' of oesophageal varices. On the other hand, where resistance to transhepatic portal flow is mild but splanchnic blood flow is markedly hyperdynamic, reducing inflow may be sufficient to ameliorate extreme portal hypertension and its sequelae.", "contents": "Relationship of splanchnic blood flow and portal venous resistance to elevated portal pressure in the dog. To determine the relationship of splanchnic blood flow and portal venous resistance to elevated portal pressure, in situ perfusion of the splanchnic circuit was carried out in 12 freshly killed dogs at varying perfusion rates and degrees of acute and chronic portal vein constriction. In six normal dogs before being killed, portal pressure averaged 8-8 +/- 1-2 mmHg and portal flow 658 +/- 81 ml/min. In the absence of portal vein constriction, increasing splanchnic perfusion to 1800 ml/min, minimally raised portal pressure (12-8 +/- 1-5 mmHg). With progressive acute constriction of the portal vein, however, comparable perfusion rates lead to progressively higher portal pressure levels and with greater than 90% constriction, portal pressure was greater than 30 mmHg with minimal elevation in splanchnic flow rate. In six other dogs before being killed but live to nine weeks after placement of an ameroid constrictor on the portal vein, portal pressure averaged 13-6 +/- 1-4 mmHg or slightly higher than in normal dogs (P greater than 0-02). Mesenteric venography and necropsy findings uniformly demonstrated 90% occlusion of the portal vein with extensive portasystemic collateralization. With increased perfusion of the splanchnic bed, portal pressure rose rapidly to approximately 35 mmHg with a flow rate of 1 800 ml/min.. These data suggest that, in disorders where resistance to transhepatic portal flow is marked, a small increment in splanchnic blood flow, which normally exerts little or no influence on portal pressure, promotes profound portal hypertension and may account for spontaneous 'rupture' of oesophageal varices. On the other hand, where resistance to transhepatic portal flow is mild but splanchnic blood flow is markedly hyperdynamic, reducing inflow may be sufficient to ameliorate extreme portal hypertension and its sequelae."}
{"id": "PMID:1083353", "title": "Hypersensitivity reactions in the small intestine. 2. Effects of allograft rejection on mucosal architecture and lymphoid cell infiltrate.", "content": "Small intestinal mucosa contains both thymus dependent and thymus independent lymphoid cells and thus has the capacity to act via humoral and cellular mechanisms as a site of local immunity and local hypersensitivity. Allograft rejection of mouse small intestine is a model of a local cell mediated reaction. The effects of this clearly defined, immunologically mediated damage villi, crypts, enterocytes, and lymphoid cell infiltrate have been assessed by comparing the morphology of rejecting allografts with that of isografts and normal small intestine of the same age. In rejection there is infiltration of the lamina propria with lymphocytes, hyperplasia of the crypts of Lieberkuhn, and an eventual sloughing off of the mucosa. Usually, but not always, there is villous atrophy and increased numbers of intraepithelial lymphocytes. However, the morphology of individual enterocytes remains normal throughout rejection and neither plasma cells nor polymorphonuclear leucocytes infiltrate the lamina propria before mucosal ulceration. These results show unequivocally that a local T cell mediated immune response causes villous atrophy and crypt hyperplasia in this animal model, and since there is no evidence of local enterocyte cytotoxicity, a lymphokine may be the link between the activated T cell and the effects on mucosal architecture. We suggest that a local CMI reaction may be the cause of villous atrophy, crypt hyperplasia, and malabsorption in many clinical and experimental conditions, including coeliac disease, food allergy, and intestinal infections.", "contents": "Hypersensitivity reactions in the small intestine. 2. Effects of allograft rejection on mucosal architecture and lymphoid cell infiltrate. Small intestinal mucosa contains both thymus dependent and thymus independent lymphoid cells and thus has the capacity to act via humoral and cellular mechanisms as a site of local immunity and local hypersensitivity. Allograft rejection of mouse small intestine is a model of a local cell mediated reaction. The effects of this clearly defined, immunologically mediated damage villi, crypts, enterocytes, and lymphoid cell infiltrate have been assessed by comparing the morphology of rejecting allografts with that of isografts and normal small intestine of the same age. In rejection there is infiltration of the lamina propria with lymphocytes, hyperplasia of the crypts of Lieberkuhn, and an eventual sloughing off of the mucosa. Usually, but not always, there is villous atrophy and increased numbers of intraepithelial lymphocytes. However, the morphology of individual enterocytes remains normal throughout rejection and neither plasma cells nor polymorphonuclear leucocytes infiltrate the lamina propria before mucosal ulceration. These results show unequivocally that a local T cell mediated immune response causes villous atrophy and crypt hyperplasia in this animal model, and since there is no evidence of local enterocyte cytotoxicity, a lymphokine may be the link between the activated T cell and the effects on mucosal architecture. We suggest that a local CMI reaction may be the cause of villous atrophy, crypt hyperplasia, and malabsorption in many clinical and experimental conditions, including coeliac disease, food allergy, and intestinal infections."}
{"id": "PMID:1083368", "title": "Distribution of alpha-1-antitrypsin phenotypes in two Dutch population groups.", "content": "The alpha1-antitrypsin phenotypes of two Dutch population groups (consisting of 672 and 802 individuals) were determined by the isoelectric focusing technique, which due to its recent development, has been used for the first time in large-scale phenotyping. As in other population studies on the alpha1-antitrypsin phenotype distribution, Pi M is the most frequently occurring allele. The two investigated groups exhibit remarkable differences, both to other studied groups as well as to each other. The most interesting results are probably the high frequencies of the alleles Pi- and of the recently discovered Pi MN. Comparison with phenotype studies carried out in other populations is also presented.", "contents": "Distribution of alpha-1-antitrypsin phenotypes in two Dutch population groups. The alpha1-antitrypsin phenotypes of two Dutch population groups (consisting of 672 and 802 individuals) were determined by the isoelectric focusing technique, which due to its recent development, has been used for the first time in large-scale phenotyping. As in other population studies on the alpha1-antitrypsin phenotype distribution, Pi M is the most frequently occurring allele. The two investigated groups exhibit remarkable differences, both to other studied groups as well as to each other. The most interesting results are probably the high frequencies of the alleles Pi- and of the recently discovered Pi MN. Comparison with phenotype studies carried out in other populations is also presented."}
{"id": "PMID:1083369", "title": "The linkage relationships of HL-A with other genetic marker systems.", "content": "Data on the linkage relationships of HL-A with 31 autosomal genetic markers are presented. Among 40 markers for which some information on the linkage relationship with HL-A is available, four (PGM3, GBG, Ch and Pg) have lod scores greater than +3. The most likely order for the polymorphic systems tentatively assigned to chromosome 6 is Kell: Pg:(HL-A,GBG):Ch:PGM3:P.", "contents": "The linkage relationships of HL-A with other genetic marker systems. Data on the linkage relationships of HL-A with 31 autosomal genetic markers are presented. Among 40 markers for which some information on the linkage relationship with HL-A is available, four (PGM3, GBG, Ch and Pg) have lod scores greater than +3. The most likely order for the polymorphic systems tentatively assigned to chromosome 6 is Kell: Pg:(HL-A,GBG):Ch:PGM3:P."}
{"id": "PMID:1083370", "title": "Utilization of uridine for RNA synthesis in the insect cell line CP-1268 derived from the codling moth, Laspeyresia pomonella.", "content": "The utilization of [3H]-5-uridine by CP-1268 cells was studied. Uridine was rapidly transported into these cells by a concentration dependent, saturable process. Exogenous uridine rapidly equilibrated with cellular nucleotide pools and virtually all of the uridine transported into the cells was phosphorylated. Uridine incorporation into RNA was studied by continuous and pulse-labeling techniques in the prescence or absence of actinomycin D and cordycepin. These studies have shown that the pattern of unstable RNA precursor and relatively stable RNA product relationship known to exist in mammalian cells similarly exists in insect cells in vitro. This pattern varied markedly with pulse-labeling time and required the addition of RNA inhibitors to block reincorporation of intracellular labeled metabolites during the chase.", "contents": "Utilization of uridine for RNA synthesis in the insect cell line CP-1268 derived from the codling moth, Laspeyresia pomonella. The utilization of [3H]-5-uridine by CP-1268 cells was studied. Uridine was rapidly transported into these cells by a concentration dependent, saturable process. Exogenous uridine rapidly equilibrated with cellular nucleotide pools and virtually all of the uridine transported into the cells was phosphorylated. Uridine incorporation into RNA was studied by continuous and pulse-labeling techniques in the prescence or absence of actinomycin D and cordycepin. These studies have shown that the pattern of unstable RNA precursor and relatively stable RNA product relationship known to exist in mammalian cells similarly exists in insect cells in vitro. This pattern varied markedly with pulse-labeling time and required the addition of RNA inhibitors to block reincorporation of intracellular labeled metabolites during the chase."}
{"id": "PMID:1083371", "title": "Effects of aprotinin on organ cultures of the rat's kidney.", "content": "Fragments of adult renal tissue were maintained as organ cultures for 7 days in HEPES-buffered medium 199, supplemented with 10% calf serum and antibiotics. Addition of aprotinin (10,000 kallikrein inhibitor units per ml) to the medium resulted in improved survival of the cells of the glomeruli and tubules and preserved the integrity of the glomerular, capsular and tubular basement membranes. Optimal results were obtained when aprotinin was present throughout the period of culturing. Inclusion of aprotinin in the medium for only the first 3 days in vitro slightly increased the numbers of surviving glomerular and tubular cells, but also promoted the growth of connective tissue in the explants and was detrimental to the basement membranes of the tubules. It is suggested that both the antiproteolytic and the carbohydrate-binding properties of aprotinin are involved in the mediation of the observed effects.", "contents": "Effects of aprotinin on organ cultures of the rat's kidney. Fragments of adult renal tissue were maintained as organ cultures for 7 days in HEPES-buffered medium 199, supplemented with 10% calf serum and antibiotics. Addition of aprotinin (10,000 kallikrein inhibitor units per ml) to the medium resulted in improved survival of the cells of the glomeruli and tubules and preserved the integrity of the glomerular, capsular and tubular basement membranes. Optimal results were obtained when aprotinin was present throughout the period of culturing. Inclusion of aprotinin in the medium for only the first 3 days in vitro slightly increased the numbers of surviving glomerular and tubular cells, but also promoted the growth of connective tissue in the explants and was detrimental to the basement membranes of the tubules. It is suggested that both the antiproteolytic and the carbohydrate-binding properties of aprotinin are involved in the mediation of the observed effects."}
{"id": "PMID:1083374", "title": "The detection of F(ab')2-related surface antigens on the thymocytes of children.", "content": "A Fowl antiserum to human polyclonal F(ab')2 with specificities for variable region antigens of both light and heavy chains and for the constant portion of the Fd fragment bound to about 20 per cent of thymocytes from three children. Binding was completely inhibited by purified human polyclonal IgG and only partly by L chains. The fowl anti-F(ab')2 also bound to a subpopulation of peripheral blood lymphocytes which was somewhat larger than the B-cell population identified with other anti-immunoglobulin sera. Binding was detected by autoradiography and the amount of radiolabel seen on thymocytes was nearly as great as that on peripheral blood lymphocytes. As no binding to thymocytes was seen with other anti-immunoglobulin sera we propose that the fowl anti-F(ab')2 recognizes immunoglobulin or material with structural similarities which is synthesized by thymocytes but of which predominantly V region determinants are exposed on the membrane surface.", "contents": "The detection of F(ab')2-related surface antigens on the thymocytes of children. A Fowl antiserum to human polyclonal F(ab')2 with specificities for variable region antigens of both light and heavy chains and for the constant portion of the Fd fragment bound to about 20 per cent of thymocytes from three children. Binding was completely inhibited by purified human polyclonal IgG and only partly by L chains. The fowl anti-F(ab')2 also bound to a subpopulation of peripheral blood lymphocytes which was somewhat larger than the B-cell population identified with other anti-immunoglobulin sera. Binding was detected by autoradiography and the amount of radiolabel seen on thymocytes was nearly as great as that on peripheral blood lymphocytes. As no binding to thymocytes was seen with other anti-immunoglobulin sera we propose that the fowl anti-F(ab')2 recognizes immunoglobulin or material with structural similarities which is synthesized by thymocytes but of which predominantly V region determinants are exposed on the membrane surface."}
{"id": "PMID:1083376", "title": "Restoration of delayed hypersensitivity to sheep erythrocytes by thymosin treatment of T-cell-depleted mice.", "content": "Calf thymosin was injected subcutaneously in daily doses of 0.1 to 3 mg for 12 to 15 days into adult thymectomized, irradiated, bone marrow-reconstituted (THXB) mice. Thymosin partially restored the ability of the T-cell-depleted host to develop delayed-type hypersensitivity to sheep erythrocytes. The degree of restoration varied from 50 to 75% of control values. Thymosin treatment of normal mice potentiated the footpad responsiveness to sheep erythrocytes by as much as 50% over that of untreated controls. The optimum dosage of thymosin seemed to be in the 200- to 500-mug range, and multiple injections were essential for a significant response. Tweleve daily injections of 100 to 500 mug of thymosin restored T-cell reactivity to the THXB mouse, but the responsiveness decayed relatively rapidly once the treatment was stopped. The restoration of immune responsiveness to sheep erythrocytes in T-cell-depleted mice provides a convenient means of demonstrating activity in thymosin preparations in vivo.", "contents": "Restoration of delayed hypersensitivity to sheep erythrocytes by thymosin treatment of T-cell-depleted mice. Calf thymosin was injected subcutaneously in daily doses of 0.1 to 3 mg for 12 to 15 days into adult thymectomized, irradiated, bone marrow-reconstituted (THXB) mice. Thymosin partially restored the ability of the T-cell-depleted host to develop delayed-type hypersensitivity to sheep erythrocytes. The degree of restoration varied from 50 to 75% of control values. Thymosin treatment of normal mice potentiated the footpad responsiveness to sheep erythrocytes by as much as 50% over that of untreated controls. The optimum dosage of thymosin seemed to be in the 200- to 500-mug range, and multiple injections were essential for a significant response. Tweleve daily injections of 100 to 500 mug of thymosin restored T-cell reactivity to the THXB mouse, but the responsiveness decayed relatively rapidly once the treatment was stopped. The restoration of immune responsiveness to sheep erythrocytes in T-cell-depleted mice provides a convenient means of demonstrating activity in thymosin preparations in vivo."}
{"id": "PMID:1083377", "title": "Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro.", "content": "Polyribophosphate, the capsular polysaccharide antigen of Haemophilus influenzae type b, can be assayed in crude bacterial extracts by a combination of immunoprecipitation, hot-acid extraction, and colorimetry. In vitro, the antigen is synthesized during growth and, in well-buffered media, in early stationary phase as well. It is shed from the cell throughout the course of synthesis, and at 37 C almost all the extractable antigen has been released spontaneously within several hours after synthesis. Various strains are similar in extent of synthesis and gross composition of the antigen; however, strains differ in the rate of release during exponential phase. The antigen is released as (or as part of) a very large structure, although under certain culture conditions much smaller antigenic units are found.", "contents": "Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro. Polyribophosphate, the capsular polysaccharide antigen of Haemophilus influenzae type b, can be assayed in crude bacterial extracts by a combination of immunoprecipitation, hot-acid extraction, and colorimetry. In vitro, the antigen is synthesized during growth and, in well-buffered media, in early stationary phase as well. It is shed from the cell throughout the course of synthesis, and at 37 C almost all the extractable antigen has been released spontaneously within several hours after synthesis. Various strains are similar in extent of synthesis and gross composition of the antigen; however, strains differ in the rate of release during exponential phase. The antigen is released as (or as part of) a very large structure, although under certain culture conditions much smaller antigenic units are found."}
{"id": "PMID:1083379", "title": "Defective repair of gamma-ray induced DNA damage in xeroderma pigmentosum cells.", "content": "We used the bromouracil-photolysis technique to estimate the sizes of the repaired regions in normal human and xeroderma pigmentosum (XP) cells irradiated by gamma-rays aerobically or anoxically. After 1 1/2 hours of incubation, single-strand breaks were repaired and the repaired regions were small--one to two BrUra residues--for cells irradiated aerobically or anoxically. After a 20-hour incubation, the repaired region in normal cells showed a component mimicking U.V.-repair. There were large patches (approximately 30 BrUra residues) in the approximate ratios of one per six chain breaks for aerobic irradiation and one per three chain breaks for anoxic irradiation. XP cells, however, only showed large patches at 20 hours if they had been irradiated aerobically. We could not detect such regions in XP cells irradiated anoxically. These results indicate (1) that some part of ionizing damage mimics excision of U.V. damage in that the repair patches are large and the repair takes an appreciable time; (2) the types of such damage depend on whether the irradiation is done aerobically or anoxically; and (3) XP cells are defective in repairing a component of anoxic damage.", "contents": "Defective repair of gamma-ray induced DNA damage in xeroderma pigmentosum cells. We used the bromouracil-photolysis technique to estimate the sizes of the repaired regions in normal human and xeroderma pigmentosum (XP) cells irradiated by gamma-rays aerobically or anoxically. After 1 1/2 hours of incubation, single-strand breaks were repaired and the repaired regions were small--one to two BrUra residues--for cells irradiated aerobically or anoxically. After a 20-hour incubation, the repaired region in normal cells showed a component mimicking U.V.-repair. There were large patches (approximately 30 BrUra residues) in the approximate ratios of one per six chain breaks for aerobic irradiation and one per three chain breaks for anoxic irradiation. XP cells, however, only showed large patches at 20 hours if they had been irradiated aerobically. We could not detect such regions in XP cells irradiated anoxically. These results indicate (1) that some part of ionizing damage mimics excision of U.V. damage in that the repair patches are large and the repair takes an appreciable time; (2) the types of such damage depend on whether the irradiation is done aerobically or anoxically; and (3) XP cells are defective in repairing a component of anoxic damage."}
{"id": "PMID:1083380", "title": "Effect of 60Co gamma-irradiation on the reaction of mixed disulphides of mercaptoethylguanidine with enzymes of rat-liver cytoplasm.", "content": "A theoretical assumption of Eldjarn and Pihl suggests that mixed disulphides formed by radioprotective aminothiols and protein SH-groups can be broken down by enzymes in the organism, and the native structure of the macromolecules restored. Irradiation should enhance this effect. In our experiments, mixed disulphides of mercaptoethylguanidine (MEG) and albumin/haemoglobin were split by the soluble enzyme fraction of rat-liver homogenate (cytosol). The liberation of the radioprotector MEG is brought about by small molecules; dialysed cytosol has no effect, nor has the suspension of particles of mitochondria. On irradiation with doses in the 0-1--5-0 Mrad range, the mixed disulphide bridge is stabilized and made more resistant to splitting. Increased resistance up to 700 per cent with albumin-MEG and 160 per cent with haemoglobin (Hb)-MEG mixed disulphide was observed compared with the unirradiated control.", "contents": "Effect of 60Co gamma-irradiation on the reaction of mixed disulphides of mercaptoethylguanidine with enzymes of rat-liver cytoplasm. A theoretical assumption of Eldjarn and Pihl suggests that mixed disulphides formed by radioprotective aminothiols and protein SH-groups can be broken down by enzymes in the organism, and the native structure of the macromolecules restored. Irradiation should enhance this effect. In our experiments, mixed disulphides of mercaptoethylguanidine (MEG) and albumin/haemoglobin were split by the soluble enzyme fraction of rat-liver homogenate (cytosol). The liberation of the radioprotector MEG is brought about by small molecules; dialysed cytosol has no effect, nor has the suspension of particles of mitochondria. On irradiation with doses in the 0-1--5-0 Mrad range, the mixed disulphide bridge is stabilized and made more resistant to splitting. Increased resistance up to 700 per cent with albumin-MEG and 160 per cent with haemoglobin (Hb)-MEG mixed disulphide was observed compared with the unirradiated control."}
{"id": "PMID:1083381", "title": "Effects of 60Co irradiation of the early developmental stages of an ixodid tick, Hyalomma anatolicum.", "content": "Various developmental stages of Hyalomma anatolicum were subjected to a range of doses of 60Co radiation from 0-10 000 R, and their subsequent development was observed. Some of these ticks were infected with Theileria annulata. Doses of 2000 R and over prevented all but a very limited development of ticks. After 1000 R a proportion of exposed individuals continued development in a near-normal manner. Lower doses had proportionately less effect. 1000 R to ticks infected with T. annulata failed to prevent transmission of typical theileriasis to calves.", "contents": "Effects of 60Co irradiation of the early developmental stages of an ixodid tick, Hyalomma anatolicum. Various developmental stages of Hyalomma anatolicum were subjected to a range of doses of 60Co radiation from 0-10 000 R, and their subsequent development was observed. Some of these ticks were infected with Theileria annulata. Doses of 2000 R and over prevented all but a very limited development of ticks. After 1000 R a proportion of exposed individuals continued development in a near-normal manner. Lower doses had proportionately less effect. 1000 R to ticks infected with T. annulata failed to prevent transmission of typical theileriasis to calves."}
{"id": "PMID:1083382", "title": "Chromosome aberrations induced in human lymphocytes by neutron irradiation.", "content": "In vitro dose--response curves of unstable chromosome aberrations in human lymphocytes have been obtained for neutron spectra of mean energies 0-7, 0-9, 7-6 and 14-7 MeV. The aberration yields have been fitted to the quadratic function Y = alphaD + betaD2, which is consistent with the single-track and two-track model of aberration formation. However with high-LET radiation, the linear component of yield, corresponding to damage caused by single tracks, predominants, and this term becomes more dominant with increasing LET, so that for fission spectrum neutrons the relationship is linear, Y = alphaD. At low doses, such as those recieved by radiation workers, limiting r.b.e. values between 13 and 47 are obtained relative to 60Co gamma-radiation. At higher doses, as used in radiotherapy, the values are much lower; ranging from 2-7 to 8 at 200 rad of equivalent gamma-radiation. Both sets of r.b.e. values correlate well with track-averaged LET but not with dose-averaged LET. When the numbers of cells without aberrations are plotted against radiation dose, curves are obtained which are similar in shape to those for conventional cell-survival experiments with comparable neutron spectra. The Do values obtained in the present study are close to those from other cell system.", "contents": "Chromosome aberrations induced in human lymphocytes by neutron irradiation. In vitro dose--response curves of unstable chromosome aberrations in human lymphocytes have been obtained for neutron spectra of mean energies 0-7, 0-9, 7-6 and 14-7 MeV. The aberration yields have been fitted to the quadratic function Y = alphaD + betaD2, which is consistent with the single-track and two-track model of aberration formation. However with high-LET radiation, the linear component of yield, corresponding to damage caused by single tracks, predominants, and this term becomes more dominant with increasing LET, so that for fission spectrum neutrons the relationship is linear, Y = alphaD. At low doses, such as those recieved by radiation workers, limiting r.b.e. values between 13 and 47 are obtained relative to 60Co gamma-radiation. At higher doses, as used in radiotherapy, the values are much lower; ranging from 2-7 to 8 at 200 rad of equivalent gamma-radiation. Both sets of r.b.e. values correlate well with track-averaged LET but not with dose-averaged LET. When the numbers of cells without aberrations are plotted against radiation dose, curves are obtained which are similar in shape to those for conventional cell-survival experiments with comparable neutron spectra. The Do values obtained in the present study are close to those from other cell system."}
{"id": "PMID:1083386", "title": "Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme.", "content": "A nucleoside phosphotransferase, which catalyzes the phosphorylation of nucleosides to nucleotides by low energy phosphate esters, has been isolated and purified 500-fold from the membrane fraction of Erwinia herbicola. Its most noteworthy difference from other enzymes of this class is that it is membrane bound and can be isolated and handled only in the presence of a detergent. With a ribonucleoside acceptor, adenosine, the reaction product is exclusively 5'-AMP; with deoxyadenosine, 5'- and 3'-nucleotide products appear in the approximate ratio of 2:1, respectively. The enzyme has no detectable phosphatase activity with the best phosphate donors, 5'-dAMP and 5'-dTMP, and very little with less active donors, such as p-nitrophenyl phosphate. This phosphotransferase should be a useful agent for preparing 5'-nucleotides from unusual synthetic bases.", "contents": "Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme. A nucleoside phosphotransferase, which catalyzes the phosphorylation of nucleosides to nucleotides by low energy phosphate esters, has been isolated and purified 500-fold from the membrane fraction of Erwinia herbicola. Its most noteworthy difference from other enzymes of this class is that it is membrane bound and can be isolated and handled only in the presence of a detergent. With a ribonucleoside acceptor, adenosine, the reaction product is exclusively 5'-AMP; with deoxyadenosine, 5'- and 3'-nucleotide products appear in the approximate ratio of 2:1, respectively. The enzyme has no detectable phosphatase activity with the best phosphate donors, 5'-dAMP and 5'-dTMP, and very little with less active donors, such as p-nitrophenyl phosphate. This phosphotransferase should be a useful agent for preparing 5'-nucleotides from unusual synthetic bases."}
{"id": "PMID:1083390", "title": "The current status of angiography in gastrointestinal bleeding.", "content": "Endoscopy, barium studies and angiography are all valuable diagnostic procedures in gastrointestinal bleeding. They are not mutually exclusive procedures but the correct sequence of investigation varies. Correct localization can be achieved in 84% of cases of massive gastrointestinal bleeding by their combined use. Vasoconstrictive therapy is also possible in severely ill patients and other poor surgical risk candidates.", "contents": "The current status of angiography in gastrointestinal bleeding. Endoscopy, barium studies and angiography are all valuable diagnostic procedures in gastrointestinal bleeding. They are not mutually exclusive procedures but the correct sequence of investigation varies. Correct localization can be achieved in 84% of cases of massive gastrointestinal bleeding by their combined use. Vasoconstrictive therapy is also possible in severely ill patients and other poor surgical risk candidates."}
{"id": "PMID:1083394", "title": "The nature of the cells generating B-lymphocyte colonies in vitro.", "content": "B-lymphocyte colonies are grown in semi-solid agar from mouse spleen or lymph node cells in the presence of mercaptoethanol with or without added sheep red cells. High levels of colony-forming cells were present in the spleen or normal mice and nu/nu (athymic) mice but colony-forming cells were rare in the thymus and not detected in activated T-lymphocyte populations. Colony-forming cells were theta-negative and most exhibited Fc receptors. Most colony-forming cells had the sedimentation velocity of small lymphocytes, were non-adherent and had a buoyant density similar to B-lymphocytes. Colony-forming cells were radiosensitive (Do60 rads) and sensitive to cortisone. Colony formation was potentiated by the addition of adherent spleen cells or peritoneal macrophages. It is concluded that most cells forming B-lymphocyte colonies are themselves characterisable as B-lymphocytes.", "contents": "The nature of the cells generating B-lymphocyte colonies in vitro. B-lymphocyte colonies are grown in semi-solid agar from mouse spleen or lymph node cells in the presence of mercaptoethanol with or without added sheep red cells. High levels of colony-forming cells were present in the spleen or normal mice and nu/nu (athymic) mice but colony-forming cells were rare in the thymus and not detected in activated T-lymphocyte populations. Colony-forming cells were theta-negative and most exhibited Fc receptors. Most colony-forming cells had the sedimentation velocity of small lymphocytes, were non-adherent and had a buoyant density similar to B-lymphocytes. Colony-forming cells were radiosensitive (Do60 rads) and sensitive to cortisone. Colony formation was potentiated by the addition of adherent spleen cells or peritoneal macrophages. It is concluded that most cells forming B-lymphocyte colonies are themselves characterisable as B-lymphocytes."}
{"id": "PMID:1083395", "title": "1,25-Dihydroxycholecalciferol deficiency: the probable cause of hypocalcemia and metabolic bone disease in pseudohypoparathyroidism.", "content": "Pseudohypoparathyroidism (PsH) is a genetic disease characterized by hypocalcemia, hyperphosphatemia, and metabolic unresponsiveness to parathyroid hormone (PTH). The administration of PTH elicits neither a significant rise in serum calcium (calcemic response) nor a decrease in the renal tubule reabsorption of phosphorus (phosphaturic response). The diminished phosphaturic response is due to an inability of PTH to generate cyclic AMP in renal tubule cells. We investigated the question of whether hypocalcemia and deficient calcemic response to PTH are due to a similar cyclic AMP defect in bone or to an acquired vitamin D deficiency. Four patients were studied. The active form of vitamin D (1,25-dihydroxycholecalciferol) was measured in 3 and was low. Treatment with vitamin D2 restored the serum calcium and the calcemic response to PTH to normal without changing the impaired renal response. Bone biopsy was performed in 2 patients and showed morphologic evidence of increased osteoclastic activity and osteomalacia. The data indicate that the hypocalcemia and bone disease in PsH are due to active vitamin D deficiency, possibly resulting from the genetic renal lesion.", "contents": "1,25-Dihydroxycholecalciferol deficiency: the probable cause of hypocalcemia and metabolic bone disease in pseudohypoparathyroidism. Pseudohypoparathyroidism (PsH) is a genetic disease characterized by hypocalcemia, hyperphosphatemia, and metabolic unresponsiveness to parathyroid hormone (PTH). The administration of PTH elicits neither a significant rise in serum calcium (calcemic response) nor a decrease in the renal tubule reabsorption of phosphorus (phosphaturic response). The diminished phosphaturic response is due to an inability of PTH to generate cyclic AMP in renal tubule cells. We investigated the question of whether hypocalcemia and deficient calcemic response to PTH are due to a similar cyclic AMP defect in bone or to an acquired vitamin D deficiency. Four patients were studied. The active form of vitamin D (1,25-dihydroxycholecalciferol) was measured in 3 and was low. Treatment with vitamin D2 restored the serum calcium and the calcemic response to PTH to normal without changing the impaired renal response. Bone biopsy was performed in 2 patients and showed morphologic evidence of increased osteoclastic activity and osteomalacia. The data indicate that the hypocalcemia and bone disease in PsH are due to active vitamin D deficiency, possibly resulting from the genetic renal lesion."}
{"id": "PMID:1083392", "title": "Emergency coronary surgery: Evolving indications.", "content": "Between December 1970 and October 1973, 74 patients underwent emergency myocardial revascularization for the relief of acute coronary insufficiency. Through our experience in this area, we have established three categories for which we perform emergency surgery. The first of these is cardiogenic shock complicating acute myocardial infarction for which 14 patients underwent emergency saphenous vein grafting. Seven of these patients expired during operation and one died three months after operation. The second category is acute unstable myocardial infarction for which 25 patients received surgery. There were no operative deaths in this group, but two patients died at six weeks and four months after operation. Thrity-five patients were classified as pre-infarctional angina, the third category for which we perform surgery on an emergency basis. There was one operative death and two late deaths at four and ten months in this group of patients. Our experience suggests that immediate coronary angiography and revascularization will improve survival in the three above-named categories.", "contents": "Emergency coronary surgery: Evolving indications. Between December 1970 and October 1973, 74 patients underwent emergency myocardial revascularization for the relief of acute coronary insufficiency. Through our experience in this area, we have established three categories for which we perform emergency surgery. The first of these is cardiogenic shock complicating acute myocardial infarction for which 14 patients underwent emergency saphenous vein grafting. Seven of these patients expired during operation and one died three months after operation. The second category is acute unstable myocardial infarction for which 25 patients received surgery. There were no operative deaths in this group, but two patients died at six weeks and four months after operation. Thrity-five patients were classified as pre-infarctional angina, the third category for which we perform surgery on an emergency basis. There was one operative death and two late deaths at four and ten months in this group of patients. Our experience suggests that immediate coronary angiography and revascularization will improve survival in the three above-named categories."}
{"id": "PMID:1083391", "title": "Limits of coronary artery surgery. Clinical and postmortem findings.", "content": "In the first series of 90 continuous patients with coronary artery disease 9 patients died after aorto-coronary bypass procedures (10%). There were 7 operative deaths, and 2 postoperative deaths (respiratory failure after bronchopneumonia and bleeding duodenal ulcer; acute necrosis of the liver following hepatitis). The study of the deceased patients made evident that postoperative impairment of left ventricular function is caused by ventricular aneurysms. This fact can be shown by the poor ventricular function with an elevated left ventricular enddiastolic pressure (LVEDP) of more than 18 mm Hg. The results in patients with congestive heart failure could not be improved by multiple bypass-grafts. Probably the prolonged surgical intervention may cause additional stress to the predamaged myocardium. So, in our group the indication for using multiple grafts in cases with ventricular aneurysm is confirmed with great caution.", "contents": "Limits of coronary artery surgery. Clinical and postmortem findings. In the first series of 90 continuous patients with coronary artery disease 9 patients died after aorto-coronary bypass procedures (10%). There were 7 operative deaths, and 2 postoperative deaths (respiratory failure after bronchopneumonia and bleeding duodenal ulcer; acute necrosis of the liver following hepatitis). The study of the deceased patients made evident that postoperative impairment of left ventricular function is caused by ventricular aneurysms. This fact can be shown by the poor ventricular function with an elevated left ventricular enddiastolic pressure (LVEDP) of more than 18 mm Hg. The results in patients with congestive heart failure could not be improved by multiple bypass-grafts. Probably the prolonged surgical intervention may cause additional stress to the predamaged myocardium. So, in our group the indication for using multiple grafts in cases with ventricular aneurysm is confirmed with great caution."}
{"id": "PMID:1083396", "title": "Mechanisms responsible for defective human T-lymphocyte sheep erythrocyte rosette function associated with hepatitis B virus infections.", "content": "The expression of selected lymphocyte surface-membrane markers was evaluated in 37 patients with acute viral hepatitis B, 10 of whom were studied serially through the resolving and convalescent phases of disease. Bone marrow-derived (B) lymphocytes were identified by reference to surface immunoglobulin, whereas normal thymus-derived (T) lymphocytes were assayed by their capacity to form spontaneous nonimmune rosettes with sheep erythrocytes (E rosettes, ER). During the acute and resolving phases of viral hepatitis B, the relative and absolute number of ER-positive lymphocytes was significantly reduced, whereas the number of surface immunoglobulin-positive lymphocytes and the absolute lymphocyte count remained normal. This resulted in the appearance of a third population of cells, deficient in respect to both surface immunoglobulin and ER markers. Such cells accounted for nearly 25% of peripheral blood lymphocytes, approximately 5 x 105ml blood. Depression of the number of ER-positive lymphocytes occurred at least once during the course of disease in every patient studied serially, and was observed in 55 of 67 individual assays of the 37 cases of acute viral hepatitis B. Lymphocytes from some patients reacquired ER function when cultured in fetal calf serum but not in the presence of autologous serum. Such autologous serum was capable of suppressing ER function of lymphocytes from normal donors. The extrinsic suppression of er function by a serum factor (designated as the Rosette Inhibitory Factor), was found to be time dependent, characterized by a 4-h latent period and requiring approximately 18 h for maximum attenuation of ER function. The Serum Rosette Inhibitory Factor was: (a) heat and freeze-thaw stable, (b) nondialyzable, (c) physically separable from hepatitis B surface antigen, (d) not a lymphocytotoxic antibody, and (e) had the buoyant density of a lipoprotein. This extrinsic mechanism was observed in 41.8% of patients with reduced numbers of ER-positive lymphocytes. The Rosette Inhibitory Factor was not detectable in the serum of the remaining 58.2% of the cases of acute and resolving viral hepatitis B despite the presence of reduced numbers of ER-positive lymphocytes. The lymphocytes from these cases did not reacquire ER function when cultured in the absence of autologous serum. The mechanisms responsible for the suppression of normal ER function in these cases appears to be intrinsic to the lymphocytes and not the result of a humoral factor. The T lymphocyte lineage of cells deficient in respect to ER function, whether of intrinsic or extrinsic type, was demonstrated by their capacity to form spontaneous rosettes with neuraminidase-treated sheep erythrocytes. Both intrinsic and extrinsic suppression of T lymphocyte ER function commonly occurred during the first 4 wk of acute viral hepatitis B.9 of the 10 patients followed serially continued to manifest defective ER function at 12 wk...", "contents": "Mechanisms responsible for defective human T-lymphocyte sheep erythrocyte rosette function associated with hepatitis B virus infections. The expression of selected lymphocyte surface-membrane markers was evaluated in 37 patients with acute viral hepatitis B, 10 of whom were studied serially through the resolving and convalescent phases of disease. Bone marrow-derived (B) lymphocytes were identified by reference to surface immunoglobulin, whereas normal thymus-derived (T) lymphocytes were assayed by their capacity to form spontaneous nonimmune rosettes with sheep erythrocytes (E rosettes, ER). During the acute and resolving phases of viral hepatitis B, the relative and absolute number of ER-positive lymphocytes was significantly reduced, whereas the number of surface immunoglobulin-positive lymphocytes and the absolute lymphocyte count remained normal. This resulted in the appearance of a third population of cells, deficient in respect to both surface immunoglobulin and ER markers. Such cells accounted for nearly 25% of peripheral blood lymphocytes, approximately 5 x 105ml blood. Depression of the number of ER-positive lymphocytes occurred at least once during the course of disease in every patient studied serially, and was observed in 55 of 67 individual assays of the 37 cases of acute viral hepatitis B. Lymphocytes from some patients reacquired ER function when cultured in fetal calf serum but not in the presence of autologous serum. Such autologous serum was capable of suppressing ER function of lymphocytes from normal donors. The extrinsic suppression of er function by a serum factor (designated as the Rosette Inhibitory Factor), was found to be time dependent, characterized by a 4-h latent period and requiring approximately 18 h for maximum attenuation of ER function. The Serum Rosette Inhibitory Factor was: (a) heat and freeze-thaw stable, (b) nondialyzable, (c) physically separable from hepatitis B surface antigen, (d) not a lymphocytotoxic antibody, and (e) had the buoyant density of a lipoprotein. This extrinsic mechanism was observed in 41.8% of patients with reduced numbers of ER-positive lymphocytes. The Rosette Inhibitory Factor was not detectable in the serum of the remaining 58.2% of the cases of acute and resolving viral hepatitis B despite the presence of reduced numbers of ER-positive lymphocytes. The lymphocytes from these cases did not reacquire ER function when cultured in the absence of autologous serum. The mechanisms responsible for the suppression of normal ER function in these cases appears to be intrinsic to the lymphocytes and not the result of a humoral factor. The T lymphocyte lineage of cells deficient in respect to ER function, whether of intrinsic or extrinsic type, was demonstrated by their capacity to form spontaneous rosettes with neuraminidase-treated sheep erythrocytes. Both intrinsic and extrinsic suppression of T lymphocyte ER function commonly occurred during the first 4 wk of acute viral hepatitis B.9 of the 10 patients followed serially continued to manifest defective ER function at 12 wk..."}
{"id": "PMID:1083397", "title": "Failure of heavy chain glycosylation of IgG in some patients with common, variable agammaglobulinemia.", "content": "Four patients with common, variable agammaglobulinemia were preveiously reported to have normal numbers of circulating B lymphocytes which synthesized normal amounts of IgG in tissue culture but failed to secrete the newly synthesized IgG. The B lymphocytes of these patients fail to incorporate [3H]mannose and/or [3H]glucosamine into newly synthesized IgG, whereas such incorporation appears to occur just before IgG secretion in cultures of normal B lymphocytes.", "contents": "Failure of heavy chain glycosylation of IgG in some patients with common, variable agammaglobulinemia. Four patients with common, variable agammaglobulinemia were preveiously reported to have normal numbers of circulating B lymphocytes which synthesized normal amounts of IgG in tissue culture but failed to secrete the newly synthesized IgG. The B lymphocytes of these patients fail to incorporate [3H]mannose and/or [3H]glucosamine into newly synthesized IgG, whereas such incorporation appears to occur just before IgG secretion in cultures of normal B lymphocytes."}
{"id": "PMID:1083398", "title": "Perfusion therapy for skin cancer.", "content": "Surgical techniques for the selective administration of anti-cancer drugs is presented. These isolated-perfusion or intra-arterial infusion procedures have achieved significant palliation for many patients with advanced cancer. When used in conjunction with surgical excision of certain early skin cancers, such as aggressive forms of malignant melanoma of the extremities, improved cure rates may be achieved.", "contents": "Perfusion therapy for skin cancer. Surgical techniques for the selective administration of anti-cancer drugs is presented. These isolated-perfusion or intra-arterial infusion procedures have achieved significant palliation for many patients with advanced cancer. When used in conjunction with surgical excision of certain early skin cancers, such as aggressive forms of malignant melanoma of the extremities, improved cure rates may be achieved."}
{"id": "PMID:1083399", "title": "Host factors and antibody response Haemophilus influenza type b meningitis and epiglottitis.", "content": "Levels of antibody in serum after infection with Haemophilus influenza type b or challenge with polysaccharide vaccine are highly variable. Convalescent-phase serum antibody to the capsular polysaccharide of H. influenzae type b was measured in two groups of patients with pathophysiologically distinct diseases, meningitis and acute epiglottitis. Antibody response after H. influenzae meningitis was subnormal. Mean levels of antibody, the distribution of antibody levels by age, and erythrocyte and genetic marker lymphocyte antigens were studied; all results suggested that these two groups of patients were genetically and immunologically different from each other. Evidence suggested that the magnitude of the important host immunologic response was under host genetic control.", "contents": "Host factors and antibody response Haemophilus influenza type b meningitis and epiglottitis. Levels of antibody in serum after infection with Haemophilus influenza type b or challenge with polysaccharide vaccine are highly variable. Convalescent-phase serum antibody to the capsular polysaccharide of H. influenzae type b was measured in two groups of patients with pathophysiologically distinct diseases, meningitis and acute epiglottitis. Antibody response after H. influenzae meningitis was subnormal. Mean levels of antibody, the distribution of antibody levels by age, and erythrocyte and genetic marker lymphocyte antigens were studied; all results suggested that these two groups of patients were genetically and immunologically different from each other. Evidence suggested that the magnitude of the important host immunologic response was under host genetic control."}
{"id": "PMID:1083400", "title": "Stimulation of lymphocytes by purified protein derivative: suppression by cells from human vaccinated with Bacille Calmette-Gu\u00e9rin.", "content": "Specific stimulation of thymus-derived cells by purified protein derivative or tetanus toxoid was inhibited by autologous lymphocytes. The lymphocytes, which were collected from healthy humans who had been vaccinated with bacille Calmette-Gu\u00e9rin were purified on nylon columns after removal of cells that form rosettes with sheep erythrocytes. The degree of purification of the lymphocytes was controlled by appropriate techniques.", "contents": "Stimulation of lymphocytes by purified protein derivative: suppression by cells from human vaccinated with Bacille Calmette-Gu\u00e9rin. Specific stimulation of thymus-derived cells by purified protein derivative or tetanus toxoid was inhibited by autologous lymphocytes. The lymphocytes, which were collected from healthy humans who had been vaccinated with bacille Calmette-Gu\u00e9rin were purified on nylon columns after removal of cells that form rosettes with sheep erythrocytes. The degree of purification of the lymphocytes was controlled by appropriate techniques."}
{"id": "PMID:1083417", "title": "Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes.", "content": "Three populations of murine splenic B lymphocytes have been characterized previously (6, 7, 9) as those bearing only IgM, those bearing only IgD, and a population bearing both isotopes. These studies were designed to test the response of the IgM+ cells (IgM-only or IgM plus IgD) vs. the IgD-only cells to the B-cell mitogen, lipopolysaccharide. Results that after 1-4 days of culture, in the presence of mitogen, the IgM+ cells enlarge and elaborate an IgM polyclonal response. The IgD-only cells, in contrast, do not exhibit an IgM polyclonal response, but instead undergo blastogenesis and proliferation.", "contents": "Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes. Three populations of murine splenic B lymphocytes have been characterized previously (6, 7, 9) as those bearing only IgM, those bearing only IgD, and a population bearing both isotopes. These studies were designed to test the response of the IgM+ cells (IgM-only or IgM plus IgD) vs. the IgD-only cells to the B-cell mitogen, lipopolysaccharide. Results that after 1-4 days of culture, in the presence of mitogen, the IgM+ cells enlarge and elaborate an IgM polyclonal response. The IgD-only cells, in contrast, do not exhibit an IgM polyclonal response, but instead undergo blastogenesis and proliferation."}
{"id": "PMID:1083418", "title": "The class of surface immunoglobulin on cells carrying IgG memory in rat thoracic duct lymph: the size of the subpopulation mediating IgG memory.", "content": "The fluorescence activated cell sorter was used to determine the class of immunoglobulin on the thoracic duct lymphocytes (TDL) which carried IgG memory. Although only about 3% of all TDL carried membrane IgG these cells accounted for most, if not all, of the adoptive IgG anti-DNP response. It is concluded that both CR+ and CR- cells mediating IgG memory in rat TDL bear the same class of membrane immunoglobulin as that secreted by their differentiated progeny. The class of membrane immunoglobulin on CR+ and CR- rat TDL was also examined. It was found that IgM+ cells, which made up over 80% of all Ig+ cells, were virtually all CR+. In contrast, the few percent of IgG+ and IgA+ cells present were to be found in both subpopulations. There was no evidence of a large population of B cells bearing exclusively heavy chains other than IgA, IgG, of IgM. The observation that some IgG+ cells as well as IgM+ cells possess a receptor for C3 appears to rule out the hypothesis that this receptor is involved in blocking a switch from IgM to IgG synthesis.", "contents": "The class of surface immunoglobulin on cells carrying IgG memory in rat thoracic duct lymph: the size of the subpopulation mediating IgG memory. The fluorescence activated cell sorter was used to determine the class of immunoglobulin on the thoracic duct lymphocytes (TDL) which carried IgG memory. Although only about 3% of all TDL carried membrane IgG these cells accounted for most, if not all, of the adoptive IgG anti-DNP response. It is concluded that both CR+ and CR- cells mediating IgG memory in rat TDL bear the same class of membrane immunoglobulin as that secreted by their differentiated progeny. The class of membrane immunoglobulin on CR+ and CR- rat TDL was also examined. It was found that IgM+ cells, which made up over 80% of all Ig+ cells, were virtually all CR+. In contrast, the few percent of IgG+ and IgA+ cells present were to be found in both subpopulations. There was no evidence of a large population of B cells bearing exclusively heavy chains other than IgA, IgG, of IgM. The observation that some IgG+ cells as well as IgM+ cells possess a receptor for C3 appears to rule out the hypothesis that this receptor is involved in blocking a switch from IgM to IgG synthesis."}
{"id": "PMID:1083419", "title": "Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient.", "content": "A 5-20% Ficoll velocity sedimentation gradient has been successfully applied to separate concanavalin A (Con A)-induced helper; and suppressor T cells. When titrated into a constant number of fresh normal spleen cells responding to sheep erythrocytes, cells from the top pool show stimulatory effects while those from the bottom pool show inhibitory activity. Both activities are found to be Con A dependent and anti-theta sensitive. We conclude that Con A-induced helper and suppressor T cells are distinct subpopulations and such separation will allow further characterization of these cell types.", "contents": "Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient. A 5-20% Ficoll velocity sedimentation gradient has been successfully applied to separate concanavalin A (Con A)-induced helper; and suppressor T cells. When titrated into a constant number of fresh normal spleen cells responding to sheep erythrocytes, cells from the top pool show stimulatory effects while those from the bottom pool show inhibitory activity. Both activities are found to be Con A dependent and anti-theta sensitive. We conclude that Con A-induced helper and suppressor T cells are distinct subpopulations and such separation will allow further characterization of these cell types."}
{"id": "PMID:1083420", "title": "Suppressor cells: dependence on assay conditions for functional activity.", "content": "Spleen cells educated in vitro with sheep red blood cells (SRBC) suppressed the plaque-forming cell response of Mishell-Dutton assay cultures challenged with optimal doses of SRBC. Changing conditions in the assay cultures changed the effect educated cells had on the assay culture responses. For example, educated cells helped rather than suppressed assay cultures of suboptimal numbers of spleen cells. Similarly, augmentation resulted upon addition of educated cells to assay cultures challenged with suboptimal doses of SRBC. Such a reversal of regulatory effects was not observed when assay cultures were challenged with supraoptimal antigen doses. Educated cells helped assay cultures of B spleen cells, and the addition of normal T cells reinstated suppression. Furthermore, maintenance of assay cultures under stationary rather than the usual rocking conditions allowed educated cells to help rather than suppress the antibody response of assay cultures. These results show that when the response of the target population (assay cultures) is low, the regulator (educated) cells augment the response, and vice versa, supporting the hypothesis that the effect regulator cells produce depends on the activity of the cells they regulate.", "contents": "Suppressor cells: dependence on assay conditions for functional activity. Spleen cells educated in vitro with sheep red blood cells (SRBC) suppressed the plaque-forming cell response of Mishell-Dutton assay cultures challenged with optimal doses of SRBC. Changing conditions in the assay cultures changed the effect educated cells had on the assay culture responses. For example, educated cells helped rather than suppressed assay cultures of suboptimal numbers of spleen cells. Similarly, augmentation resulted upon addition of educated cells to assay cultures challenged with suboptimal doses of SRBC. Such a reversal of regulatory effects was not observed when assay cultures were challenged with supraoptimal antigen doses. Educated cells helped assay cultures of B spleen cells, and the addition of normal T cells reinstated suppression. Furthermore, maintenance of assay cultures under stationary rather than the usual rocking conditions allowed educated cells to help rather than suppress the antibody response of assay cultures. These results show that when the response of the target population (assay cultures) is low, the regulator (educated) cells augment the response, and vice versa, supporting the hypothesis that the effect regulator cells produce depends on the activity of the cells they regulate."}
{"id": "PMID:1083421", "title": "Growth of B-lymphocytes clones in semisolid culture is mitogen dependent.", "content": "A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B lymphocytes.", "contents": "Growth of B-lymphocytes clones in semisolid culture is mitogen dependent. A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B lymphocytes."}
{"id": "PMID:1083422", "title": "Cross-priming for a secondary cytotoxic response to minor H antigens with H-2 congenic cells which do not cross-react in the cytotoxic assay.", "content": "Cytotoxic effector T cells of F1 (BALB/c X BALB.B) (H-2d/b) mice immunized against the minor histocompatibility differences of C57BL/10 (H-2b) can lyse targets from C57BL/10, but cannot lyse B10.D2 (H-2d) targets. Despite this lack of cross-reaction in the cytotoxic assay, C57BL/10 cells do prime F1 (BALB/c X BALB.B) mice for a secondary cytotoxic response to B10.D2. C57BL/10-primed, B10.D2-boosted cytotoxic cells lyse B10.D2 targets but not C57BL/10 targets. DBA/2 (H-2d) spleen cells or thymocytes prime F1 mice for a secondary response to DBA/2, B10.D2, and C57BL/10 cells, but DBA/2 mastocytes, P815, do not prime for a response to C57BL/10. Whether H-2 congenic lymphoid cells express minor histocompatibility determinants which cross-react at the cytotoxic T-cell level or the helper T-cell level is discussed.", "contents": "Cross-priming for a secondary cytotoxic response to minor H antigens with H-2 congenic cells which do not cross-react in the cytotoxic assay. Cytotoxic effector T cells of F1 (BALB/c X BALB.B) (H-2d/b) mice immunized against the minor histocompatibility differences of C57BL/10 (H-2b) can lyse targets from C57BL/10, but cannot lyse B10.D2 (H-2d) targets. Despite this lack of cross-reaction in the cytotoxic assay, C57BL/10 cells do prime F1 (BALB/c X BALB.B) mice for a secondary cytotoxic response to B10.D2. C57BL/10-primed, B10.D2-boosted cytotoxic cells lyse B10.D2 targets but not C57BL/10 targets. DBA/2 (H-2d) spleen cells or thymocytes prime F1 mice for a secondary response to DBA/2, B10.D2, and C57BL/10 cells, but DBA/2 mastocytes, P815, do not prime for a response to C57BL/10. Whether H-2 congenic lymphoid cells express minor histocompatibility determinants which cross-react at the cytotoxic T-cell level or the helper T-cell level is discussed."}
{"id": "PMID:1083424", "title": "An improved vaseline gap voltage clamp for skeletal muscle fibers.", "content": "A Vaseline gap potentiometric recording and voltage clamp method is developed for frog skeletal muscle fibers. The method is based on the Frankenhaeuser-Dodge voltage clamp for myelinated nerve with modifications to improve the frequency response, to compensate for external series resistance, and to compensate for the complex impedance of the current-passing pathway. Fragments of single muscle fibers are plucked from the semitendinosus muscle and mounted while depolarized by a solution like CsF. After Vaseline seals are formed between fluid pools, the fiber ends are cut once again, the central region is rinsed with Ringer solution, and the feedback amplifiers are turned on. Errors in the potential and current records are assessed by direct measurements with microelectrodes. The passive properties of the preparation are simulated by the \"disk\" equivalent circuit for the transverse tubular system and the derived parameters are similar to previous measurements with microelectrodes. Action potentials at 5 degrees C are long because of the absence of delayed rectification. Their shape is approximately simulated by solving the disk model with sodium permeability in the surface and tubular membranes. Voltage clamp currents consist primarily of capacity currents and sodium currents. The peak inward sodium current density at 5 degrees C is 3.7 mA/cm2. At 5 degrees C the sodium currents are smoothly graded with increasing depolarization and free of notches suggesting good control of the surface membrane. At higher temperatures a small, late extra inward current appears for small depolarizations that has the properties expected for excitation in the transverse tubular system. Comparison of recorded currents with simulations shows that while the transverse tubular system has regenerative sodium currents, they are too small to make important errors in the total current recorded at the surface under voltage clamp at low temperature. The tubules are definitely not under voltage clamp control.", "contents": "An improved vaseline gap voltage clamp for skeletal muscle fibers. A Vaseline gap potentiometric recording and voltage clamp method is developed for frog skeletal muscle fibers. The method is based on the Frankenhaeuser-Dodge voltage clamp for myelinated nerve with modifications to improve the frequency response, to compensate for external series resistance, and to compensate for the complex impedance of the current-passing pathway. Fragments of single muscle fibers are plucked from the semitendinosus muscle and mounted while depolarized by a solution like CsF. After Vaseline seals are formed between fluid pools, the fiber ends are cut once again, the central region is rinsed with Ringer solution, and the feedback amplifiers are turned on. Errors in the potential and current records are assessed by direct measurements with microelectrodes. The passive properties of the preparation are simulated by the \"disk\" equivalent circuit for the transverse tubular system and the derived parameters are similar to previous measurements with microelectrodes. Action potentials at 5 degrees C are long because of the absence of delayed rectification. Their shape is approximately simulated by solving the disk model with sodium permeability in the surface and tubular membranes. Voltage clamp currents consist primarily of capacity currents and sodium currents. The peak inward sodium current density at 5 degrees C is 3.7 mA/cm2. At 5 degrees C the sodium currents are smoothly graded with increasing depolarization and free of notches suggesting good control of the surface membrane. At higher temperatures a small, late extra inward current appears for small depolarizations that has the properties expected for excitation in the transverse tubular system. Comparison of recorded currents with simulations shows that while the transverse tubular system has regenerative sodium currents, they are too small to make important errors in the total current recorded at the surface under voltage clamp at low temperature. The tubules are definitely not under voltage clamp control."}
{"id": "PMID:1083427", "title": "Orbicularis oculi reflex and facial muscle electromyography. Pre- and postoperative evaluation of posterior fossa space-occupying lesions.", "content": "The electrically evoked orbicularis oculi reflex was analyzed in 26 patients with proven posterior fossa space-occupying lesions (11 cerebellopontine angle and 15 cerebellar hemispheric masses). In the former group, although only six had clinical evidence of trigeminal or facial involvement, the reflex analysis showed both nerves to be involved in all. Electromyography (EMG) of the orbicularis oculi showed profound denervation of this muscle while only minimal abnormalities of the masseter were seen. The sensory component of the trigeminal nerve was always more involved than its motor component and this was a reliable guide as to the upward and medial extension of the tumor, especially in the absence of clinical localizing signs. In the 15 patients with cerebellar masses, six with masses located posteriorly and entirely within the confines of the cerebellum had normal studies. In eight, the mass was located anteriorly, and fifth nerve involvement could be detected in all by EMG, although only two patients had clinical evidence of such involvement. One patient with dysfunction of the central reflex arc had EMG evidence of an intraventricular dermoid tumor abutting the pons, later confirmed at surgery. Thus, an invaluable body of diagnostic and prognostic data can be obtained by this nontraumatic and simple electrodiagnostic procedure.", "contents": "Orbicularis oculi reflex and facial muscle electromyography. Pre- and postoperative evaluation of posterior fossa space-occupying lesions. The electrically evoked orbicularis oculi reflex was analyzed in 26 patients with proven posterior fossa space-occupying lesions (11 cerebellopontine angle and 15 cerebellar hemispheric masses). In the former group, although only six had clinical evidence of trigeminal or facial involvement, the reflex analysis showed both nerves to be involved in all. Electromyography (EMG) of the orbicularis oculi showed profound denervation of this muscle while only minimal abnormalities of the masseter were seen. The sensory component of the trigeminal nerve was always more involved than its motor component and this was a reliable guide as to the upward and medial extension of the tumor, especially in the absence of clinical localizing signs. In the 15 patients with cerebellar masses, six with masses located posteriorly and entirely within the confines of the cerebellum had normal studies. In eight, the mass was located anteriorly, and fifth nerve involvement could be detected in all by EMG, although only two patients had clinical evidence of such involvement. One patient with dysfunction of the central reflex arc had EMG evidence of an intraventricular dermoid tumor abutting the pons, later confirmed at surgery. Thus, an invaluable body of diagnostic and prognostic data can be obtained by this nontraumatic and simple electrodiagnostic procedure."}
{"id": "PMID:1083430", "title": "Characteristics of the entry process for sodium in transporting epithelia as revealed with amiloride.", "content": "1. The permeation of sodium ions trhough the mucosal surface of frog skin epithelium at different transepithelial potentials has been investigated using the blocking drug amiloride. 2. An increase in serosal negativity in voltage-clamped skins was associated with an increase in the absolute amount of inhibition caused by a fixed concentration of amiloride. Hyperpolarizing or depolarizing skins with respect to the short-circuited condition did not affect the apparent affinity of amiloride for the entry sites. 3. When skins were voltage clamped at -50 mV (serosa negative) the specific binding of amiloride to sodium entry sites was increased by 77% compared to the short-circuited condition. Skins clamped at +50 mV had only 72% of the specific binding found in short-circuited skins. Experiments with a second blocking drug, triamterene, indicated that the extra binding sites appearing at -50mV were similar to those found under short-circuit conditions. The appearance and disappearance of binding sites may reflect changes in cell volume. 4. The findings suggest that the increased sodium current which flows when skins are clamped at -50 mV results from an increase in the number of entry sites, and perhaps also to a voltage sensitive increase in flux through each entry site.", "contents": "Characteristics of the entry process for sodium in transporting epithelia as revealed with amiloride. 1. The permeation of sodium ions trhough the mucosal surface of frog skin epithelium at different transepithelial potentials has been investigated using the blocking drug amiloride. 2. An increase in serosal negativity in voltage-clamped skins was associated with an increase in the absolute amount of inhibition caused by a fixed concentration of amiloride. Hyperpolarizing or depolarizing skins with respect to the short-circuited condition did not affect the apparent affinity of amiloride for the entry sites. 3. When skins were voltage clamped at -50 mV (serosa negative) the specific binding of amiloride to sodium entry sites was increased by 77% compared to the short-circuited condition. Skins clamped at +50 mV had only 72% of the specific binding found in short-circuited skins. Experiments with a second blocking drug, triamterene, indicated that the extra binding sites appearing at -50mV were similar to those found under short-circuit conditions. The appearance and disappearance of binding sites may reflect changes in cell volume. 4. The findings suggest that the increased sodium current which flows when skins are clamped at -50 mV results from an increase in the number of entry sites, and perhaps also to a voltage sensitive increase in flux through each entry site."}
{"id": "PMID:1083431", "title": "Separation of two voltage-sensitive potassium currents, and demonstration of a tetrodotoxin-resistant calcium current in frog motoneurones.", "content": "1. Depolarization-induced voltage and conductance changes were studied in frog montoneurones in isolated, perfused spinal cord slices. Two types of afterhyperpolarization are observed following action potentials in normal Ringer, a fast afterhyperpolarization lastin 5-10 msec and a slow afterhyperpolarization lasting 60-200 msec. Both afterhyperpolarizations are mediated by an increased K+ conductance. 2. The slow afterhyperpolarization and conductance increase underlying it are selectively and reversibly inhibited by perfusion with solutions containing low [Ca2+] (less than or equal to 0-2 nM) or the Ca2+ antagonists Mn2+ (1mM) or Co2+ (5 mM), and are enhanced by perfusion with high [Ca2+]. 3. Addition of 2-5 mM tetraethylammonium ion (TEA+) to the perfusing solution prolongs the falling phase of the action potential and abolishes the fast afterhyperpolarization, but does not inhibit the slow afterhyperolarization. 4. When the voltage-dependent Na+ current is blocked by perfusion with TTX (10-5 M), intracellularly applied depolarizing current steps evoke fast and slow hyperpolarizations with kinetics and pharmacological sensitivities similar to those of the fast and slow afterhyperpolarizations, respectively. The fast hyperpolarization is maximally activated by brief, intense depolarizations, the slow hyperpolarization by prolonged, less intense depolarizations. 5. These pharmacological and kinetic data demonstrate that in frog motoneurones the repolarization-fast afterhyperpolarization sequence and the slow afterhyperpolarization are produced by different K+ conductance systems. The fast K+ conductance activates rapidly on depolarization, decays rapidly on repolarization, and is TEA+ sensitive, while the slow K+ conducatance activates and decays more slowly and is Ca2+-dependent. 6. Motoneurones perfused with TEA+ and TEA often show a slow, regenerative depolarizing response to applied depolarizing currents. These regenerative depolarizations are probably produced by an influx of Ca2+, because they persist in isotonic CaCl2 and are blocked by Mn2+ or low [Ca2+]. The Ca2+-dependence of the slow afterhyperpolarization and the increase in slow afterhyperpolarization magnitude observed following the slow Ca2+ potentials suggest that a depolarization-evoked Ca2+ influx activates the K+ conductance underlying the slow afterhyperpolarization. 7. Motoneurones in which the slow Ca2+ and K+ conductance systems have been enhanced by high [Ca2+] or blocked by Mn2+ show altered discharge patterns in response to intracellularly applied depolarizing current steps. Perfusion with twice normal [Ca2+] (4 mM) causes montoneurones to discharge more slowly at all current intensities, and reduces the slope of the 'steady-state' frequency-current relationship. Mn2+-perfused motoneurones exhibit fairly normal high-frequency discharge at the onset of the current step, but unlike normal motoneurones, do not discharge at frequencies below 60/sec...", "contents": "Separation of two voltage-sensitive potassium currents, and demonstration of a tetrodotoxin-resistant calcium current in frog motoneurones. 1. Depolarization-induced voltage and conductance changes were studied in frog montoneurones in isolated, perfused spinal cord slices. Two types of afterhyperpolarization are observed following action potentials in normal Ringer, a fast afterhyperpolarization lastin 5-10 msec and a slow afterhyperpolarization lasting 60-200 msec. Both afterhyperpolarizations are mediated by an increased K+ conductance. 2. The slow afterhyperpolarization and conductance increase underlying it are selectively and reversibly inhibited by perfusion with solutions containing low [Ca2+] (less than or equal to 0-2 nM) or the Ca2+ antagonists Mn2+ (1mM) or Co2+ (5 mM), and are enhanced by perfusion with high [Ca2+]. 3. Addition of 2-5 mM tetraethylammonium ion (TEA+) to the perfusing solution prolongs the falling phase of the action potential and abolishes the fast afterhyperpolarization, but does not inhibit the slow afterhyperolarization. 4. When the voltage-dependent Na+ current is blocked by perfusion with TTX (10-5 M), intracellularly applied depolarizing current steps evoke fast and slow hyperpolarizations with kinetics and pharmacological sensitivities similar to those of the fast and slow afterhyperpolarizations, respectively. The fast hyperpolarization is maximally activated by brief, intense depolarizations, the slow hyperpolarization by prolonged, less intense depolarizations. 5. These pharmacological and kinetic data demonstrate that in frog motoneurones the repolarization-fast afterhyperpolarization sequence and the slow afterhyperpolarization are produced by different K+ conductance systems. The fast K+ conductance activates rapidly on depolarization, decays rapidly on repolarization, and is TEA+ sensitive, while the slow K+ conducatance activates and decays more slowly and is Ca2+-dependent. 6. Motoneurones perfused with TEA+ and TEA often show a slow, regenerative depolarizing response to applied depolarizing currents. These regenerative depolarizations are probably produced by an influx of Ca2+, because they persist in isotonic CaCl2 and are blocked by Mn2+ or low [Ca2+]. The Ca2+-dependence of the slow afterhyperpolarization and the increase in slow afterhyperpolarization magnitude observed following the slow Ca2+ potentials suggest that a depolarization-evoked Ca2+ influx activates the K+ conductance underlying the slow afterhyperpolarization. 7. Motoneurones in which the slow Ca2+ and K+ conductance systems have been enhanced by high [Ca2+] or blocked by Mn2+ show altered discharge patterns in response to intracellularly applied depolarizing current steps. Perfusion with twice normal [Ca2+] (4 mM) causes montoneurones to discharge more slowly at all current intensities, and reduces the slope of the 'steady-state' frequency-current relationship. Mn2+-perfused motoneurones exhibit fairly normal high-frequency discharge at the onset of the current step, but unlike normal motoneurones, do not discharge at frequencies below 60/sec..."}
{"id": "PMID:1083429", "title": "Relationship between Monro's foramen and cranial breadth: a method for a more accurate echo-encephalographic examination.", "content": "A pneumoencephalographic study to localize the position of Monro's foramen in the skull cavity is reported. This has to be done by evaluating the place of Monro's foramen in both vertical and horizontal plane. A method to perform more accurate ultrasonic examinations is described.", "contents": "Relationship between Monro's foramen and cranial breadth: a method for a more accurate echo-encephalographic examination. A pneumoencephalographic study to localize the position of Monro's foramen in the skull cavity is reported. This has to be done by evaluating the place of Monro's foramen in both vertical and horizontal plane. A method to perform more accurate ultrasonic examinations is described."}
{"id": "PMID:1083432", "title": "[Tetanic fatigue and proximate post-tetanic recuperation in sartorius and flexor carpi radialis muscles of the male frog. Effects of iodoacetic acid (author's transl)].", "content": "The time-course of the isometric tension output, at 20 degrees C, during a long tetanus and after a short period of rest, was investigated in two isolated frog muscles : the sartorius and flexor carpi radialis muscles. To prevent aerobie and glycolytic recovery processes, some muscles were poisoned with 0,4 mM iodoacetic acid (IAA) and nitrogen, for 20 or 40 min. 1. For the unpoisoned sartorius muscle, tetanic tension declined quickly, but after a 0,8 sec period of rest, the muscle was able to develop high tension. Poisoning with IAA-N2 increased fatigue without suppressing the property of a proximate post-tetanic recuperation. 2. In the flexor carpi radialis muscle resistance to fatigue was very large before poisoning and diminished after poisoning. Proximate recuperation was very weak. 3. The results show that the recovery processes are not a primary factor of the development of the short-term fatigue ; they enhance the hypothesis that a failure of the electromechanical coupling can explain the rate of the tension fall in tetanized sartorius muscles.", "contents": "[Tetanic fatigue and proximate post-tetanic recuperation in sartorius and flexor carpi radialis muscles of the male frog. Effects of iodoacetic acid (author's transl)]. The time-course of the isometric tension output, at 20 degrees C, during a long tetanus and after a short period of rest, was investigated in two isolated frog muscles : the sartorius and flexor carpi radialis muscles. To prevent aerobie and glycolytic recovery processes, some muscles were poisoned with 0,4 mM iodoacetic acid (IAA) and nitrogen, for 20 or 40 min. 1. For the unpoisoned sartorius muscle, tetanic tension declined quickly, but after a 0,8 sec period of rest, the muscle was able to develop high tension. Poisoning with IAA-N2 increased fatigue without suppressing the property of a proximate post-tetanic recuperation. 2. In the flexor carpi radialis muscle resistance to fatigue was very large before poisoning and diminished after poisoning. Proximate recuperation was very weak. 3. The results show that the recovery processes are not a primary factor of the development of the short-term fatigue ; they enhance the hypothesis that a failure of the electromechanical coupling can explain the rate of the tension fall in tetanized sartorius muscles."}
{"id": "PMID:1083434", "title": "Introducing quality assurance and medical audit into the UCSF medical center curriculum.", "content": "The experience gained by a medical school faculty in developing and piloting a course for undergraduate medical students in medical care evaluation led to a similar effort for house staff. It is recognized that if the profession is to fulfill the demand by society for social accountability in the use of resources for health care, medical care assessment and quality assurance mechanisms must become an intimate part of the clinical experience of medical students and house officers. Teaching these subjects requires a theoretical framework; introduction of content and skills appropriate to the level of the student and continuation of progressively more advanced training throughout medical education; use of assessment and quality assurance techniques by clinician-teachers themselves to provide models for the student; and continued evolution of pedagogic approach and course content based on developments in the area.", "contents": "Introducing quality assurance and medical audit into the UCSF medical center curriculum. The experience gained by a medical school faculty in developing and piloting a course for undergraduate medical students in medical care evaluation led to a similar effort for house staff. It is recognized that if the profession is to fulfill the demand by society for social accountability in the use of resources for health care, medical care assessment and quality assurance mechanisms must become an intimate part of the clinical experience of medical students and house officers. Teaching these subjects requires a theoretical framework; introduction of content and skills appropriate to the level of the student and continuation of progressively more advanced training throughout medical education; use of assessment and quality assurance techniques by clinician-teachers themselves to provide models for the student; and continued evolution of pedagogic approach and course content based on developments in the area."}
{"id": "PMID:1083435", "title": "Rickets in Nigerian children--response to vitamin D.", "content": "The findings in 20 cases of nutritional rickets treated on an out-patient basis with oral calciferol and calcium gluconate are reported. Attention was paid to their clinical, biochemical and radiological responses. If was noted that presentation at the hospital out-patient department was always delayed until there were obvious and sometimes severe deformities in  the children. Biochemical changes noted during the early phase of treatment showed an initial fall in the level of serum calcium and plasma phosohorus and a rise in the serum alkaline phosphatase. About three months after initiating treatment, calcium and phosphorus reverted to normal levels but serum alkaline phosphatase still remained high. Twelve of our cases (60 per cent) suffered from protein calorie malnutrition of the marasmic type and showed an apparent resistance to vitamin D therapy in what may be regarded as normal dosages. Response was however, achieved with substantial increase in the amount of vitamin given. There was a striking sex difference in the ratio of 15 males to five females indicating that males are more likely to develop nutritional rickets than females when subjected to predisposing environmental conditions. Vitamin D resistant type of rickets was not encountered in this study.", "contents": "Rickets in Nigerian children--response to vitamin D. The findings in 20 cases of nutritional rickets treated on an out-patient basis with oral calciferol and calcium gluconate are reported. Attention was paid to their clinical, biochemical and radiological responses. If was noted that presentation at the hospital out-patient department was always delayed until there were obvious and sometimes severe deformities in  the children. Biochemical changes noted during the early phase of treatment showed an initial fall in the level of serum calcium and plasma phosohorus and a rise in the serum alkaline phosphatase. About three months after initiating treatment, calcium and phosphorus reverted to normal levels but serum alkaline phosphatase still remained high. Twelve of our cases (60 per cent) suffered from protein calorie malnutrition of the marasmic type and showed an apparent resistance to vitamin D therapy in what may be regarded as normal dosages. Response was however, achieved with substantial increase in the amount of vitamin given. There was a striking sex difference in the ratio of 15 males to five females indicating that males are more likely to develop nutritional rickets than females when subjected to predisposing environmental conditions. Vitamin D resistant type of rickets was not encountered in this study."}
{"id": "PMID:1083437", "title": "Carcinogenesis in tissue culture 25: reduced tumorigenicity of alkaline phosphatase-constitutive variants from Chinese hamster ovary cells.", "content": "Several subclones with high alkaline phosphatase (ALP) activity were isolated from ALP-negative Chinese hamster ovary cells (CHO-K1). When inoculated into cheek pouch membranes of Syrian golden hamsters treated with anti-hamster thymocyte serum (ATS), ALP-negative CHO-K1 cells produced progressively growing tumors, whereas the cells of ALP-positive subclones did not, although small nodules were formed temporarily. The animals injected CHO-K1 cells died of tumor by 35 days after grafting, and metastases in liver and lung were revealed on autopsy. The histological features of the resultant neoplasms were consistent with fibrosarcoma. In animals transplanted with the cells of ALP-positive subclones, neither tumor death, nor metastasis formation was observed.", "contents": "Carcinogenesis in tissue culture 25: reduced tumorigenicity of alkaline phosphatase-constitutive variants from Chinese hamster ovary cells. Several subclones with high alkaline phosphatase (ALP) activity were isolated from ALP-negative Chinese hamster ovary cells (CHO-K1). When inoculated into cheek pouch membranes of Syrian golden hamsters treated with anti-hamster thymocyte serum (ATS), ALP-negative CHO-K1 cells produced progressively growing tumors, whereas the cells of ALP-positive subclones did not, although small nodules were formed temporarily. The animals injected CHO-K1 cells died of tumor by 35 days after grafting, and metastases in liver and lung were revealed on autopsy. The histological features of the resultant neoplasms were consistent with fibrosarcoma. In animals transplanted with the cells of ALP-positive subclones, neither tumor death, nor metastasis formation was observed."}
{"id": "PMID:1083449", "title": "Hepatitis B antigen and systemic lupus erythematosus. False positive complement fixation due to anti-antibodies.", "content": "Sera from 22 patients with systemic lupus erythematosus (SLE) were examined for the presence of hepatitis B antigen (HBsAG) by a complement fixation (CF) test, by an immunoelectrophoretic method (counterelectrophoresis-CEP), and by radioimmunoassay (RIA). The sera from 8 patients gave positive results using CF. However, the same sera and sera from 28 additional SLE patients, when tested with CEP and RIA, were not shown to contain HBsAG.", "contents": "Hepatitis B antigen and systemic lupus erythematosus. False positive complement fixation due to anti-antibodies. Sera from 22 patients with systemic lupus erythematosus (SLE) were examined for the presence of hepatitis B antigen (HBsAG) by a complement fixation (CF) test, by an immunoelectrophoretic method (counterelectrophoresis-CEP), and by radioimmunoassay (RIA). The sera from 8 patients gave positive results using CF. However, the same sera and sera from 28 additional SLE patients, when tested with CEP and RIA, were not shown to contain HBsAG."}
{"id": "PMID:1083454", "title": "Studies of receptors on normal and malignant tissue from man.", "content": "Receptor studies on normal and malignant human tissues were carried out. Erythrocyte receptor studies failed to show differences between malignant and normal tissues; both apparently have a receptor for rat erythrocytes, a hemolysin for monkey erythrocytes, and an agglutinin for rabbit erythrocytes. Fc receptors were found in several malignant tissues and only an occasional normal tissue, except for a patient who died of sepsis.", "contents": "Studies of receptors on normal and malignant tissue from man. Receptor studies on normal and malignant human tissues were carried out. Erythrocyte receptor studies failed to show differences between malignant and normal tissues; both apparently have a receptor for rat erythrocytes, a hemolysin for monkey erythrocytes, and an agglutinin for rabbit erythrocytes. Fc receptors were found in several malignant tissues and only an occasional normal tissue, except for a patient who died of sepsis."}
{"id": "PMID:1083455", "title": "Coronary collateral circulation during stress and the effects of aorta-coronary bypass grafts.", "content": "The ability of coronary collateral vessels to supply an adequate volume of blood to the subendocardium during the resting state and during periods of stress was studied. Regional myocardial blood flow was determined by the radioactive microsphere technique. Ameroid constricting devices were placed around the left circumflex coronary artery in 22 adult mongrel dogs. Three months later, regional myocardial blood flow was measured in a normal area of the left ventricle as well as in the area supplied entirely by coronary collateral vessels. Collateral blood flow was sufficient in the resting state to prevent myocardial ischemia distal to a gradually occluded coronary artery. However, in 10 animals (Group I) without aorta-coronary bypass grafts (ACBG), a selective underperfusion of the collateralized subendocardium occurred during periods of stress. In 12 animals (Group II), this selective underperfusion of the collateralized subendocardium with stress was abolished by placing ACBG's distal to the site of the coronary artery occlusion.", "contents": "Coronary collateral circulation during stress and the effects of aorta-coronary bypass grafts. The ability of coronary collateral vessels to supply an adequate volume of blood to the subendocardium during the resting state and during periods of stress was studied. Regional myocardial blood flow was determined by the radioactive microsphere technique. Ameroid constricting devices were placed around the left circumflex coronary artery in 22 adult mongrel dogs. Three months later, regional myocardial blood flow was measured in a normal area of the left ventricle as well as in the area supplied entirely by coronary collateral vessels. Collateral blood flow was sufficient in the resting state to prevent myocardial ischemia distal to a gradually occluded coronary artery. However, in 10 animals (Group I) without aorta-coronary bypass grafts (ACBG), a selective underperfusion of the collateralized subendocardium occurred during periods of stress. In 12 animals (Group II), this selective underperfusion of the collateralized subendocardium with stress was abolished by placing ACBG's distal to the site of the coronary artery occlusion."}
{"id": "PMID:1083456", "title": "Operative flow measurements and coronary bypass graft patency.", "content": "Early (one week) and late (one year) postoperative angiography was performed in 142 patients having 310 grafts (117 right coronary artery [RCA], 134 left anterior descending [LAD], and 59 circumflex coronary artery [CCA]) to assess the factors responsible for failure of aorta-coronary artery saphenous vein grafts. Early catheterization revealed an 85.5 per cent patency rate with similar rates for each artery (RCA 88 per cent, LAD 85 per cent, and CCA 81 per cent). At one year 238 grafts remained patent, for a cumulative patency rate of 76.8 per cent with a similar distribution for each vessel (RCA 75 per cent, LAD 78 per cent, and CCA 76 per cent). Intraoperative flow measurements were correlated with early and late patency. Grafts with a basal flow less than 20 ml. per minute have a 42 per cent early closure rate and a 21 per cent late closure rate (cumulative 63 per cent). A basal flow of less than 40 ml. per minute was associated with a 25 per cent early failure and an 11 per cent late failure rate (cumulative 36 per cent). Basal flow at levels greater than 40 ml. per minute was not associated with an increased probability of graft closure. Absence of reactive hyperemia (30 second graft occlusion) was associated with a 19 per cent probability of early closure and a 31 per cent probability of cumulative thrombosis. A papaverine-induced flow increase (15 mg. given into the graft) of less than 100 per cent over basal flow gave a 20 per cent probability of early failure and 30 per cent probability of cumulative closure. Thus intraoperative basal flow measurements are of predictive value in determining the fate of aorta-coronary artery vein bypass grafts, and vasodilatory maneuvers provide little additional information.", "contents": "Operative flow measurements and coronary bypass graft patency. Early (one week) and late (one year) postoperative angiography was performed in 142 patients having 310 grafts (117 right coronary artery [RCA], 134 left anterior descending [LAD], and 59 circumflex coronary artery [CCA]) to assess the factors responsible for failure of aorta-coronary artery saphenous vein grafts. Early catheterization revealed an 85.5 per cent patency rate with similar rates for each artery (RCA 88 per cent, LAD 85 per cent, and CCA 81 per cent). At one year 238 grafts remained patent, for a cumulative patency rate of 76.8 per cent with a similar distribution for each vessel (RCA 75 per cent, LAD 78 per cent, and CCA 76 per cent). Intraoperative flow measurements were correlated with early and late patency. Grafts with a basal flow less than 20 ml. per minute have a 42 per cent early closure rate and a 21 per cent late closure rate (cumulative 63 per cent). A basal flow of less than 40 ml. per minute was associated with a 25 per cent early failure and an 11 per cent late failure rate (cumulative 36 per cent). Basal flow at levels greater than 40 ml. per minute was not associated with an increased probability of graft closure. Absence of reactive hyperemia (30 second graft occlusion) was associated with a 19 per cent probability of early closure and a 31 per cent probability of cumulative thrombosis. A papaverine-induced flow increase (15 mg. given into the graft) of less than 100 per cent over basal flow gave a 20 per cent probability of early failure and 30 per cent probability of cumulative closure. Thus intraoperative basal flow measurements are of predictive value in determining the fate of aorta-coronary artery vein bypass grafts, and vasodilatory maneuvers provide little additional information."}
{"id": "PMID:1083457", "title": "Infrarenal mycotic pseudoaneurysm. A late complication of coronary bypass surgery with proximal aortic dissection.", "content": "A 62-year-old man developed a mycotic infrarenal pseudoaneurysm at the re-entry site of a previous aortic dissection. The aortic dissection had occurred one year earlier while he was undergoing coronary bypass surgery. The patient was successfully treated with a Dacron bifurcation graft after the mycotic aneurysm had been sterilized. Causes and relationships of the mycotic pseudoaneurysm to aortic dissection and aortic balloon support are discussed.", "contents": "Infrarenal mycotic pseudoaneurysm. A late complication of coronary bypass surgery with proximal aortic dissection. A 62-year-old man developed a mycotic infrarenal pseudoaneurysm at the re-entry site of a previous aortic dissection. The aortic dissection had occurred one year earlier while he was undergoing coronary bypass surgery. The patient was successfully treated with a Dacron bifurcation graft after the mycotic aneurysm had been sterilized. Causes and relationships of the mycotic pseudoaneurysm to aortic dissection and aortic balloon support are discussed."}
{"id": "PMID:1083458", "title": "Distribution and severity of atherosclerosis in the coronary arteries.", "content": "A postmortem study of 93 human hearts was undertaken. Gross inspection was used to determine the degree of atherosclerosis and postmortem coronary angiography to estimate the degree of luminal narrowing. The findings indicate the following: (1) There is high correlation between the estimates of luminal narrowing in the gross specimen and the presence of significant atherosclerosis. (2) Approximately 30 per cent of vessels with significant proximal disease will have significant distal coronary artery disease. (3) When one coronary artery is involved with severe proximal atherosclerosis, either of the other two vessels are likely to be involved, with a frequency of 75 per cent ormore. (4) When significant distal disease is present the proximal vessel is nearly always involved. (5) Patient selection prior to referral to surgery may be partly responsible for the over 90 per cent operability rate in patients undergoing coronary artery bypass grafting.", "contents": "Distribution and severity of atherosclerosis in the coronary arteries. A postmortem study of 93 human hearts was undertaken. Gross inspection was used to determine the degree of atherosclerosis and postmortem coronary angiography to estimate the degree of luminal narrowing. The findings indicate the following: (1) There is high correlation between the estimates of luminal narrowing in the gross specimen and the presence of significant atherosclerosis. (2) Approximately 30 per cent of vessels with significant proximal disease will have significant distal coronary artery disease. (3) When one coronary artery is involved with severe proximal atherosclerosis, either of the other two vessels are likely to be involved, with a frequency of 75 per cent ormore. (4) When significant distal disease is present the proximal vessel is nearly always involved. (5) Patient selection prior to referral to surgery may be partly responsible for the over 90 per cent operability rate in patients undergoing coronary artery bypass grafting."}
{"id": "PMID:1083459", "title": "Scanning electron microscope study of human veins and aorta-coronary artery vein grafts.", "content": "Scanning electron microscope study of two vein grafts which were removed after 50 months of implantation as aorta-coronary artery vein grafts showed considerable subendothelial thickening, which consisted of relatively cell-poor fibrous layer. In one of the grafts the endothelial surface was intact; the second graft showed focal loss of endothelial cells. Sections of two fresh human veins, studied prior to their insertion as aorta-coronary artery vein grafts, have shown that they retained an intact endothelial layer. A third fresh vein, however, showed focal damage with missing endothelial cells and adherent fibrin.", "contents": "Scanning electron microscope study of human veins and aorta-coronary artery vein grafts. Scanning electron microscope study of two vein grafts which were removed after 50 months of implantation as aorta-coronary artery vein grafts showed considerable subendothelial thickening, which consisted of relatively cell-poor fibrous layer. In one of the grafts the endothelial surface was intact; the second graft showed focal loss of endothelial cells. Sections of two fresh human veins, studied prior to their insertion as aorta-coronary artery vein grafts, have shown that they retained an intact endothelial layer. A third fresh vein, however, showed focal damage with missing endothelial cells and adherent fibrin."}
{"id": "PMID:1083460", "title": "Myocardial revascularization. Early and late results after reoperation.", "content": "In a series of 4,522 consecutive patients who underwent aorta-coronary bypass (ACB) with the saphenous vein at the Texas Heart Institute, 32 had a second revascularization procedure. All patients were reoperated upon because of recurrence of incapacitating angina. Reappearance of angina was related to obstruction of the grafts alone in 6 patients, to the disease of other arteries alone in 16, and to both sources in the remaining 10 patients. In 9 patients progression of the native coronary disease was found, in 16 significant coronary obstructions had been left unbypassed at the time of initial operation, and in the remaining 7 patients inadequate indication and/or performance of revascularization was considered responsible for the failure. Of the 31 survivors, 61 per cent experienced complete relief of angina or were improved, whereas 39 per cent were unimproved. Reoperation was more successful in relieving angina when performed in patients with new lesions or with previously unbypassed lisions than when done in patients with graft occlusion. Incidence of myocardial infarction after the first and second procedure was similar (3 per cent). Reoperation was performed with a mortality rate of 3 per cent, comparable to that of the original procedure, but relief of angina was not achieved so consistently.", "contents": "Myocardial revascularization. Early and late results after reoperation. In a series of 4,522 consecutive patients who underwent aorta-coronary bypass (ACB) with the saphenous vein at the Texas Heart Institute, 32 had a second revascularization procedure. All patients were reoperated upon because of recurrence of incapacitating angina. Reappearance of angina was related to obstruction of the grafts alone in 6 patients, to the disease of other arteries alone in 16, and to both sources in the remaining 10 patients. In 9 patients progression of the native coronary disease was found, in 16 significant coronary obstructions had been left unbypassed at the time of initial operation, and in the remaining 7 patients inadequate indication and/or performance of revascularization was considered responsible for the failure. Of the 31 survivors, 61 per cent experienced complete relief of angina or were improved, whereas 39 per cent were unimproved. Reoperation was more successful in relieving angina when performed in patients with new lesions or with previously unbypassed lisions than when done in patients with graft occlusion. Incidence of myocardial infarction after the first and second procedure was similar (3 per cent). Reoperation was performed with a mortality rate of 3 per cent, comparable to that of the original procedure, but relief of angina was not achieved so consistently."}
{"id": "PMID:1083461", "title": "Hemodynamic effects of venous valves in aorto-coronary bypass grafts.", "content": "Use of the saphenous vein in aorto-coronary bypass surgery for treatment of occlusive coronary artery disease is a well-established technique. Use of the mammary artery for the same purpose has also been favorably reported. This study was undertaken to determine whether a vein graft containing a valve would have an added advantage. A comparison of the effect of competent and incompetent valves in vein grafts on the hemodynamics of coronary circulation was made. In a group of 18 dogs, a 5 cm. portion of each dog's cephalic vein containing a valve was used in a single aorto-coronary bypass graft. Flow measurements were made between the valve and the anastomosis of the graft to the left anterior descending coronary artery (LAD). The valve was made temporarily incompetent by the insertion of a spring wire basket, 3.5 mm. in diameter, through the valve via a side branch. The flow of blood through the venous graft with the competent valve was 11.0 +/- 2.6 per cent higher than with the incompetent valve. An analysis of flow patterns showed that this increased flow could be attributed to a reduction of backflow during systole and an increase in forward diastolic flow. Results suggest that postoperative myocardial perfusion is enhanced by the presence of valves in aorto-coronary bypass vein grafts.", "contents": "Hemodynamic effects of venous valves in aorto-coronary bypass grafts. Use of the saphenous vein in aorto-coronary bypass surgery for treatment of occlusive coronary artery disease is a well-established technique. Use of the mammary artery for the same purpose has also been favorably reported. This study was undertaken to determine whether a vein graft containing a valve would have an added advantage. A comparison of the effect of competent and incompetent valves in vein grafts on the hemodynamics of coronary circulation was made. In a group of 18 dogs, a 5 cm. portion of each dog's cephalic vein containing a valve was used in a single aorto-coronary bypass graft. Flow measurements were made between the valve and the anastomosis of the graft to the left anterior descending coronary artery (LAD). The valve was made temporarily incompetent by the insertion of a spring wire basket, 3.5 mm. in diameter, through the valve via a side branch. The flow of blood through the venous graft with the competent valve was 11.0 +/- 2.6 per cent higher than with the incompetent valve. An analysis of flow patterns showed that this increased flow could be attributed to a reduction of backflow during systole and an increase in forward diastolic flow. Results suggest that postoperative myocardial perfusion is enhanced by the presence of valves in aorto-coronary bypass vein grafts."}
{"id": "PMID:1083462", "title": "Alpha1-antitrypsin phenotypes in chronic active liver disease and primary biliary cirrhosis.", "content": "Alpha1-antitrypsin concentrations and phenotypes were determined in groups of patients with chronic active liver disease and primary biliary cirrhosis. The concentrations of alpha1-antitrypsin were above normal values in both groups; the patients with primary biliary cirrhosis had higher concentrations than those with chronic active liver disease. The prevalence of common phenotypes in these two groups did not differ from that in a sample of healthy blood donors from this institution of from a large Norwegian sample. We interpret our data as disputing the view that alpha1-antitrypsin phenotypes, other than Z. significantly predispose adults to hepatic cirrhosis.", "contents": "Alpha1-antitrypsin phenotypes in chronic active liver disease and primary biliary cirrhosis. Alpha1-antitrypsin concentrations and phenotypes were determined in groups of patients with chronic active liver disease and primary biliary cirrhosis. The concentrations of alpha1-antitrypsin were above normal values in both groups; the patients with primary biliary cirrhosis had higher concentrations than those with chronic active liver disease. The prevalence of common phenotypes in these two groups did not differ from that in a sample of healthy blood donors from this institution of from a large Norwegian sample. We interpret our data as disputing the view that alpha1-antitrypsin phenotypes, other than Z. significantly predispose adults to hepatic cirrhosis."}
{"id": "PMID:1083470", "title": "[Humoral and cell-bound immunological reactions in chronic hepatitis (author's transl)].", "content": "Various forms of chronic hepatitis can be differentiated on the basis of histological changes as well as of immunological findings. Humoral and cell-bound immune phenomena in chronic hepatitis are described; their significance for the pathogenesis and for establishing a diagnosis is discussed.", "contents": "[Humoral and cell-bound immunological reactions in chronic hepatitis (author's transl)]. Various forms of chronic hepatitis can be differentiated on the basis of histological changes as well as of immunological findings. Humoral and cell-bound immune phenomena in chronic hepatitis are described; their significance for the pathogenesis and for establishing a diagnosis is discussed."}
{"id": "PMID:1083474", "title": "Arterial vasoconstrictor therapy for bleeding oesophageal varices.", "content": "Upper gastrointestinal bleeding in patients with hepatic cirrhosis carries considerable mortality. Difficulties are encountered both in determining the source of bleeding and in controlling blood loss. The techniques of selective visceral angiography not only supply diagnostic information, but can be used to administer selective intra-arterial vasoconstrictor therapy to control blood loss. We report our experience with 28 patients in whom angiography was performed with particular reference to six patients treated with selective vasoconstrictor therapy. Although the precise role of the technique is not yet established, early experience is most promising. We believe it will play an important role in a difficult group of patients in the future and may well supplant present methods of controlling bleeding.", "contents": "Arterial vasoconstrictor therapy for bleeding oesophageal varices. Upper gastrointestinal bleeding in patients with hepatic cirrhosis carries considerable mortality. Difficulties are encountered both in determining the source of bleeding and in controlling blood loss. The techniques of selective visceral angiography not only supply diagnostic information, but can be used to administer selective intra-arterial vasoconstrictor therapy to control blood loss. We report our experience with 28 patients in whom angiography was performed with particular reference to six patients treated with selective vasoconstrictor therapy. Although the precise role of the technique is not yet established, early experience is most promising. We believe it will play an important role in a difficult group of patients in the future and may well supplant present methods of controlling bleeding."}
{"id": "PMID:1083475", "title": "Comparative effectiveness of tetracycline, minocycline and doxycycline in treatment of acute-on-chronic bronchitis. A study based on sputum levels.", "content": "After the administration of minocycline, doxycyline and tetracycline in recommended therapeutic doses to 11, 10 and 12 patients respectively, sputum levels of these antibiotics were determined. Only 20% of patients given doxcycline had a satisfactory sputum level and in the remainder levels were either low or absent. For patients receiving tetracycline and minocycline, 67% and 64% respectively had satisfactory sputum levels. Patients selected for this study had a history of chronic bronchitis and were producing copious amounts of purulent sputum. Minimum inhibitory concentrations of these three antibiotics were similar for 25 freshly isolated strains of Haemophilus influenzae, with minocycline being the most inhibitory and doxcycline the least inhibitory.", "contents": "Comparative effectiveness of tetracycline, minocycline and doxycycline in treatment of acute-on-chronic bronchitis. A study based on sputum levels. After the administration of minocycline, doxycyline and tetracycline in recommended therapeutic doses to 11, 10 and 12 patients respectively, sputum levels of these antibiotics were determined. Only 20% of patients given doxcycline had a satisfactory sputum level and in the remainder levels were either low or absent. For patients receiving tetracycline and minocycline, 67% and 64% respectively had satisfactory sputum levels. Patients selected for this study had a history of chronic bronchitis and were producing copious amounts of purulent sputum. Minimum inhibitory concentrations of these three antibiotics were similar for 25 freshly isolated strains of Haemophilus influenzae, with minocycline being the most inhibitory and doxcycline the least inhibitory."}
{"id": "PMID:1083476", "title": "[Therapy of tuberculosis with Rifampicin in childhood (author's transl)].", "content": "Compared with the adults the child's bloodlevel after having taken Rifampicin is lower, therefore it is recommanded to take a higher dose of 15 mg/kg in the morning before having breakfasted. A combination of doses of 10 mg/kg INH and 20 mg/kg Ethambutol is possible without any doubt. If you consider the reaction which may arise, SGOT and SGPT values up to 21 mU are without any signification. If they depasse this limit a certain control is necessary and if they are still progressive the therapy must be changed. If you compare the experiences with the adults, secondary effects of Rifampicin in childhood are less, just so indigestibleness of the stomach or intestines tract or allergies.", "contents": "[Therapy of tuberculosis with Rifampicin in childhood (author's transl)]. Compared with the adults the child's bloodlevel after having taken Rifampicin is lower, therefore it is recommanded to take a higher dose of 15 mg/kg in the morning before having breakfasted. A combination of doses of 10 mg/kg INH and 20 mg/kg Ethambutol is possible without any doubt. If you consider the reaction which may arise, SGOT and SGPT values up to 21 mU are without any signification. If they depasse this limit a certain control is necessary and if they are still progressive the therapy must be changed. If you compare the experiences with the adults, secondary effects of Rifampicin in childhood are less, just so indigestibleness of the stomach or intestines tract or allergies."}
{"id": "PMID:1083483", "title": "The variable pattern of circulating lymphocyte subpopulations in chronic lymphocytic leukemia.", "content": "The percentages of T and B lymphocytes in 13 patients with chronic lymphocytic leukemia were determined at two-week intervals. One patient with \"B-cell predominant\" disease showed a decrease from 77 to 49 in the percentage of circulating B cells, which bind heat aggregated immunoglobulin and anti-human immunoglobulin. This patient had a comparable increase in T cells, which form rosettes with sheep erythrocytes. The lymphocytes of three other patients, who originally had equal percentages of cells that bound heat-aggregated immunoglobulin and anti-human immunoglobulin, lost lg determinants from 47 to 63 per cent of cells without changing the proportion of cells with receptors for heat-aggregated immunoglobulin or for sheep erythrocytes. The characterization of lymphocytes in chronic lymphocytic leukemia should include sequential determination of cell-surface markers for both T and B cells, since the disease does not represent a proliferation of B cells alone.", "contents": "The variable pattern of circulating lymphocyte subpopulations in chronic lymphocytic leukemia. The percentages of T and B lymphocytes in 13 patients with chronic lymphocytic leukemia were determined at two-week intervals. One patient with \"B-cell predominant\" disease showed a decrease from 77 to 49 in the percentage of circulating B cells, which bind heat aggregated immunoglobulin and anti-human immunoglobulin. This patient had a comparable increase in T cells, which form rosettes with sheep erythrocytes. The lymphocytes of three other patients, who originally had equal percentages of cells that bound heat-aggregated immunoglobulin and anti-human immunoglobulin, lost lg determinants from 47 to 63 per cent of cells without changing the proportion of cells with receptors for heat-aggregated immunoglobulin or for sheep erythrocytes. The characterization of lymphocytes in chronic lymphocytic leukemia should include sequential determination of cell-surface markers for both T and B cells, since the disease does not represent a proliferation of B cells alone."}
{"id": "PMID:1083484", "title": "Influenza: response of T-cell lymphopenia to thymosin.", "content": "Eighteen volunteers in tow study groups were inoculated with influenza A (H3N2) and their peripheral blood T, B and null cells enumerated at subsequent intervals. Infection with wild-type virus or with a live, attenuated virus vaccine markedly reduced the proportion and absolute number of T-cell rosettes 24 hours after inoculation. T-Cell depression preceded the onset of clinical illness in symptomatic subjects, continued during illness, and returned to normal with recovery. T-cell lymphopenia was most pronounced in volunteers infected with wild-type virus and was accompanied by an increase in null cells. Lymphocytes from six wild-virus recipients with T-cell leukopenia were incubated in vitro with a calfthymus extract (thymosin), significantly increasing the percentage of T rosettes in all six subjects (P less than 0.0001). These data indicate that influenza is accompanied by pronounced quantitative and functional changes in T cells.", "contents": "Influenza: response of T-cell lymphopenia to thymosin. Eighteen volunteers in tow study groups were inoculated with influenza A (H3N2) and their peripheral blood T, B and null cells enumerated at subsequent intervals. Infection with wild-type virus or with a live, attenuated virus vaccine markedly reduced the proportion and absolute number of T-cell rosettes 24 hours after inoculation. T-Cell depression preceded the onset of clinical illness in symptomatic subjects, continued during illness, and returned to normal with recovery. T-cell lymphopenia was most pronounced in volunteers infected with wild-type virus and was accompanied by an increase in null cells. Lymphocytes from six wild-virus recipients with T-cell leukopenia were incubated in vitro with a calfthymus extract (thymosin), significantly increasing the percentage of T rosettes in all six subjects (P less than 0.0001). These data indicate that influenza is accompanied by pronounced quantitative and functional changes in T cells."}
{"id": "PMID:1083485", "title": "Liver disease in alpha1-antitrypsin deficiency detected by screening of 200,000 infants.", "content": "We prosepctively studied 200,000 newborns to determine the frequency and clinical characteristics of alpha1-antitrypsin deficiency. One hundred and twenty Pi Z, 48 Pi SZ, two PI Z-and one Pi S-infants were identified and followed to the age of six months. Fourteen of 120 Pi Z infants had prolonged obstructive jaundice, nine with severe clinical and laboratory evidence of liver disease. Five had only laboratory evidence of liver disease. Eight other Pi Z infants had minimal abnormalities in serum bilirubin and hepatic enzyme activity and variable hepatosplenomegaly. All 22 Pi Z infants with hepatic abnormalities, two thirds of whom were made, appeared healthy at six months of age. Ninety-eight Pi Z infants did not have clinical liver disease, but liver-function tests gave abnormal results in 44 of 84 at three months, and in 36 of 60 at six months of age. The number of small-for-gestational-age infants was greater (P less than 0.001) among those with clinical liver disease. None of the 48 Pi SZ infants had clinical liver disease, but 10 of 42 at three months and one of 22 at six months of age had abnormal liver function. The Pi Z and Pi SZ phenotypes are associated with covert or readily apparent hepatic dysfunction in the first three months of life.", "contents": "Liver disease in alpha1-antitrypsin deficiency detected by screening of 200,000 infants. We prosepctively studied 200,000 newborns to determine the frequency and clinical characteristics of alpha1-antitrypsin deficiency. One hundred and twenty Pi Z, 48 Pi SZ, two PI Z-and one Pi S-infants were identified and followed to the age of six months. Fourteen of 120 Pi Z infants had prolonged obstructive jaundice, nine with severe clinical and laboratory evidence of liver disease. Five had only laboratory evidence of liver disease. Eight other Pi Z infants had minimal abnormalities in serum bilirubin and hepatic enzyme activity and variable hepatosplenomegaly. All 22 Pi Z infants with hepatic abnormalities, two thirds of whom were made, appeared healthy at six months of age. Ninety-eight Pi Z infants did not have clinical liver disease, but liver-function tests gave abnormal results in 44 of 84 at three months, and in 36 of 60 at six months of age. The number of small-for-gestational-age infants was greater (P less than 0.001) among those with clinical liver disease. None of the 48 Pi SZ infants had clinical liver disease, but 10 of 42 at three months and one of 22 at six months of age had abnormal liver function. The Pi Z and Pi SZ phenotypes are associated with covert or readily apparent hepatic dysfunction in the first three months of life."}
{"id": "PMID:1083492", "title": "[Axonal composition of the marginal and basal optic tracts of the grass frog].", "content": "An electron-microscopy study of the marginal optic tracts (the medial and lateral divisions) and basal optic tract was performed for the frog Rana temporaria. The resemblance between the ultrastructure and composition of the marginal tracts is revealed. The basal tract is characterized by a relatively loose structure and considerable content of the myelinated large diameter fibres. The corresponding tract contains 3900 (4.7%), 4700, (4.9%), 700 (28%) of myelinated fibres and 79 800 (95.3%), 91500 (95.1%) and 1800 (72%) of unmyelinated ones. The spectra of the myelinated fibre diameters of the marginal tract are from 0.4 to 2.6 mum with the main peak at 1.0 mum and additional peak at 1.6 mum; the spectrum of the myelinated fibre diameters of the basal tract is from 0.4 to 4.0 mum with the main peak at 1.8 mum and the small additional peak at 3.2 mum. The spectra of the unmyelinated fibre diameters of all tracts are from 0.1 to 0.5 mum; 60% of them have a diameter of about 0.2 mum. 85 days after enucleation undegenerated myelinated fibres were not found in the basal tract of the frog kept at a temperature 18-20 degrees C. The medial and lateral marginal tracts contained a significant number of the undegenerated myelinated fibres, 33% and 13%, the diameter spectra being 0.6-1.4 mum and 0.6-1.0 mum, respectively. It is possible that these fibres participate in the organization of the ipsilateral visual projection on the tectum. Unmyelinated fibres remain unchanged in all the studied tracts.", "contents": "[Axonal composition of the marginal and basal optic tracts of the grass frog]. An electron-microscopy study of the marginal optic tracts (the medial and lateral divisions) and basal optic tract was performed for the frog Rana temporaria. The resemblance between the ultrastructure and composition of the marginal tracts is revealed. The basal tract is characterized by a relatively loose structure and considerable content of the myelinated large diameter fibres. The corresponding tract contains 3900 (4.7%), 4700, (4.9%), 700 (28%) of myelinated fibres and 79 800 (95.3%), 91500 (95.1%) and 1800 (72%) of unmyelinated ones. The spectra of the myelinated fibre diameters of the marginal tract are from 0.4 to 2.6 mum with the main peak at 1.0 mum and additional peak at 1.6 mum; the spectrum of the myelinated fibre diameters of the basal tract is from 0.4 to 4.0 mum with the main peak at 1.8 mum and the small additional peak at 3.2 mum. The spectra of the unmyelinated fibre diameters of all tracts are from 0.1 to 0.5 mum; 60% of them have a diameter of about 0.2 mum. 85 days after enucleation undegenerated myelinated fibres were not found in the basal tract of the frog kept at a temperature 18-20 degrees C. The medial and lateral marginal tracts contained a significant number of the undegenerated myelinated fibres, 33% and 13%, the diameter spectra being 0.6-1.4 mum and 0.6-1.0 mum, respectively. It is possible that these fibres participate in the organization of the ipsilateral visual projection on the tectum. Unmyelinated fibres remain unchanged in all the studied tracts."}
{"id": "PMID:1083494", "title": "Histiocytosis X of the cauda equina.", "content": "Laminectomy, performed on a 46-year-old woman because of weakness and paresthesias of the lower limbs, showed multiple discrete yellow nodules on the nerve roots of the cauda equina. One of these lesions was excised and proved to be a granuloma consistent with the diagnosis of histiocytosis X.", "contents": "Histiocytosis X of the cauda equina. Laminectomy, performed on a 46-year-old woman because of weakness and paresthesias of the lower limbs, showed multiple discrete yellow nodules on the nerve roots of the cauda equina. One of these lesions was excised and proved to be a granuloma consistent with the diagnosis of histiocytosis X."}
{"id": "PMID:1083495", "title": "[Benzogammapirone in the treatment of digestive hemorrhages].", "content": "Given the difficulties of treating digestive haemorhages with normal coagulant methods, and considering that most haemorrhages are due to vascular factors, a vasoprotective substance was associated with common coagulant therapies. Benzogammapirone was used orally and by selective arterial infusion. The series is presented and the results discussed.", "contents": "[Benzogammapirone in the treatment of digestive hemorrhages]. Given the difficulties of treating digestive haemorhages with normal coagulant methods, and considering that most haemorrhages are due to vascular factors, a vasoprotective substance was associated with common coagulant therapies. Benzogammapirone was used orally and by selective arterial infusion. The series is presented and the results discussed."}
{"id": "PMID:1083496", "title": "Chorioepithelioma presenting as a bleeding gingival mass.", "content": "A case is reported in which a very malignant neoplasm appeared clinically to be a pregnancy tumor or a hemangioma. There was, of course, no question as to the cause of death in this particular case. It was of interest because of the rarity of the tumor, the metastasis to the oral cavity, and the fact that the final diagnosis was established from pathologic examination. The case demonstrates, with emphasis, the great importance of biopsy and routine pathologic examination of all tissue for accurate and correct diagnosis, regardless of its clinical appearance.", "contents": "Chorioepithelioma presenting as a bleeding gingival mass. A case is reported in which a very malignant neoplasm appeared clinically to be a pregnancy tumor or a hemangioma. There was, of course, no question as to the cause of death in this particular case. It was of interest because of the rarity of the tumor, the metastasis to the oral cavity, and the fact that the final diagnosis was established from pathologic examination. The case demonstrates, with emphasis, the great importance of biopsy and routine pathologic examination of all tissue for accurate and correct diagnosis, regardless of its clinical appearance."}
{"id": "PMID:1083500", "title": "Aorta-circumflex coronary artery bypass through the transverse sinus. A new technique.", "content": "Aorta-circumflex coronary artery bypass using a saphenous vein graft may present technical inadequacies with angulation, kinking, compression, tension and finally thrombosis and occlusion of the graft. As these problems are mainly secondary to incorrect length and direction of the vein graft, a new technique is described which allows the placement of a shorter graft in a more parallel direction to the physiologic aortocoronary flow. The transverse sinus of the heart is used as a natural protected channel whereby the graft passes behind the great vessels from the right posterior wall of the aorta to the circumflex coronary artery. Thus, redundancy, angulation and kinking are avoided and flow in the graft takes a more natural course.", "contents": "Aorta-circumflex coronary artery bypass through the transverse sinus. A new technique. Aorta-circumflex coronary artery bypass using a saphenous vein graft may present technical inadequacies with angulation, kinking, compression, tension and finally thrombosis and occlusion of the graft. As these problems are mainly secondary to incorrect length and direction of the vein graft, a new technique is described which allows the placement of a shorter graft in a more parallel direction to the physiologic aortocoronary flow. The transverse sinus of the heart is used as a natural protected channel whereby the graft passes behind the great vessels from the right posterior wall of the aorta to the circumflex coronary artery. Thus, redundancy, angulation and kinking are avoided and flow in the graft takes a more natural course."}
{"id": "PMID:1083501", "title": "Antinuclear antibodies and lupus-like syndromes in children receiving anticonvulsants.", "content": "Drug-induced systemic lupus erythematosus (SLE)-like syndromes in children are most commonly associated with the administration of ethosuximide, diphenylhydantoin, and trimethadione. Five children receiving ethosuximide who presented with syndromes suggestive of SLE were studied. Each and fever, malar rash, arthritis, and lymphadenopathy. Two children had pleural effusions and another developed myocarditis and pericarditis. Three patients had anti-DNA antibodies associated with low serum C3. In four of five children symptoms disappeared with the discontinuation of ethosuximide; two of these continue to have antinuclear antibodies (ANA). One child continues to have active SLE with nephritis. A group of 101 children from a seizure clinic were tested for the presence of ANA. ANA were found in 14 of 70 children receiving ethosuximide and/or diphenylhydantoin; 2 of 14 had anti-DNA antibodies. Serum ANA titers in the drug-induced SLE group did not differ significantly from those of the asymptomatic seizure patients. ANA were also present in 5 of 23 children receiving phenobarbital only. The induction of ANA by phenobarbital is a possible hypothesis. Quantitative immunoglobulins and C3 were not significantly altered in the asymptomatic children with ANA. Follow-up studies at ten months showed no asymptomatic child with ANA to have developed clinical with ANA to have developed clinical evidence of SLE. This study suggests that asymptomatic children who develop ANA should have careful observation, but need not have their anticonvulsants discontinued.", "contents": "Antinuclear antibodies and lupus-like syndromes in children receiving anticonvulsants. Drug-induced systemic lupus erythematosus (SLE)-like syndromes in children are most commonly associated with the administration of ethosuximide, diphenylhydantoin, and trimethadione. Five children receiving ethosuximide who presented with syndromes suggestive of SLE were studied. Each and fever, malar rash, arthritis, and lymphadenopathy. Two children had pleural effusions and another developed myocarditis and pericarditis. Three patients had anti-DNA antibodies associated with low serum C3. In four of five children symptoms disappeared with the discontinuation of ethosuximide; two of these continue to have antinuclear antibodies (ANA). One child continues to have active SLE with nephritis. A group of 101 children from a seizure clinic were tested for the presence of ANA. ANA were found in 14 of 70 children receiving ethosuximide and/or diphenylhydantoin; 2 of 14 had anti-DNA antibodies. Serum ANA titers in the drug-induced SLE group did not differ significantly from those of the asymptomatic seizure patients. ANA were also present in 5 of 23 children receiving phenobarbital only. The induction of ANA by phenobarbital is a possible hypothesis. Quantitative immunoglobulins and C3 were not significantly altered in the asymptomatic children with ANA. Follow-up studies at ten months showed no asymptomatic child with ANA to have developed clinical with ANA to have developed clinical evidence of SLE. This study suggests that asymptomatic children who develop ANA should have careful observation, but need not have their anticonvulsants discontinued."}
{"id": "PMID:1083503", "title": "Chloride transport in isolated frog (Rana temporaria) skin: changes in short-circuit currently, potential, resistance, and chloride flux elicited by furosemide.", "content": "In a large number of isolated frog skins, with potential differences of from 20--92 mV (mean, 55.3 +/- 3.6 S.E.M.), the chloride influx was found to be slightly greater than chloride efflux under short-circuit conditions, but the difference was not statistically significant. However, if skins of low potential (less than 50 mV) were selected, chloride influx was significantly higher than chloride efflux (P less than 0.05). In this low potential group furosemide, (10(-3)M, applied to the solution bathing the mucosal surface) was found to produce a) a small increase in short-circuit current, which was generally apparent within 1 min, maximal in 5 min; and thereafter declined towards the control value; b) a marked increase in potential difference, apparent within 1 min and sustained for at least 30 min; c) a large and sustained increase in the calculated d.c. resistance of the skin and d) a decrease in the influx of chloride, such that influx and efflux were equalized.", "contents": "Chloride transport in isolated frog (Rana temporaria) skin: changes in short-circuit currently, potential, resistance, and chloride flux elicited by furosemide. In a large number of isolated frog skins, with potential differences of from 20--92 mV (mean, 55.3 +/- 3.6 S.E.M.), the chloride influx was found to be slightly greater than chloride efflux under short-circuit conditions, but the difference was not statistically significant. However, if skins of low potential (less than 50 mV) were selected, chloride influx was significantly higher than chloride efflux (P less than 0.05). In this low potential group furosemide, (10(-3)M, applied to the solution bathing the mucosal surface) was found to produce a) a small increase in short-circuit current, which was generally apparent within 1 min, maximal in 5 min; and thereafter declined towards the control value; b) a marked increase in potential difference, apparent within 1 min and sustained for at least 30 min; c) a large and sustained increase in the calculated d.c. resistance of the skin and d) a decrease in the influx of chloride, such that influx and efflux were equalized."}
{"id": "PMID:1083504", "title": "[Obstructive pulmonary syndromes in children with alpha 1 antitrypsin deficiency. 3 cases].", "content": "During the last six years, the search for a deficiency in alpha-1-antitrypsin (AAT) was carried out in 106 children aged from 5 months to 14 years, having the obstructive pulmonary syndrome. Nine deficiencies were in this manner detected and phenotyped. These are the observations of three of these children which are reported here. Their age ranged between 4 years and a half for two of them and 8 years for the third one, having asthma. The phenotypes are respectively SS, MZ, and ZZ. Following these observations, the problems brought up by the association in AAT deficiency and the obstructive pulmonary syndrome in children are discussed: clinical symptomatology, interpretation of the basic design, pathogenic signification and possible connections with asthma and therapeutic influence. The functional respiratory disorders noted in these children involved a ventilatory asychronism, an overexpansion, and regional abnormalities of ventilation and perfusion. To answer these still outstanding questions, the need for a systematic prospective study carried out on a wide group of children is emphasized.", "contents": "[Obstructive pulmonary syndromes in children with alpha 1 antitrypsin deficiency. 3 cases]. During the last six years, the search for a deficiency in alpha-1-antitrypsin (AAT) was carried out in 106 children aged from 5 months to 14 years, having the obstructive pulmonary syndrome. Nine deficiencies were in this manner detected and phenotyped. These are the observations of three of these children which are reported here. Their age ranged between 4 years and a half for two of them and 8 years for the third one, having asthma. The phenotypes are respectively SS, MZ, and ZZ. Following these observations, the problems brought up by the association in AAT deficiency and the obstructive pulmonary syndrome in children are discussed: clinical symptomatology, interpretation of the basic design, pathogenic signification and possible connections with asthma and therapeutic influence. The functional respiratory disorders noted in these children involved a ventilatory asychronism, an overexpansion, and regional abnormalities of ventilation and perfusion. To answer these still outstanding questions, the need for a systematic prospective study carried out on a wide group of children is emphasized."}
{"id": "PMID:1083506", "title": "[Platelet anomaly in an eosinophilic myelophroliferative syndrome].", "content": "The authors report a case of chronic eosinophilic leukemia. Ristocetin did not aggregate patient's platelets in the absence of bleeding disorder. A possible relationship between arterial thrombosis observed in patients with eosinophilic leukemia and platelet abnormality is evoked.", "contents": "[Platelet anomaly in an eosinophilic myelophroliferative syndrome]. The authors report a case of chronic eosinophilic leukemia. Ristocetin did not aggregate patient's platelets in the absence of bleeding disorder. A possible relationship between arterial thrombosis observed in patients with eosinophilic leukemia and platelet abnormality is evoked."}
{"id": "PMID:1083514", "title": "Aortocoronary bypass surgery for ischemic heart disease: indications based on controlled trials.", "content": "One- and two-year results of aortocoronary bypass for ischemic heart disease indicate that in stable angina pectoris, at least, the operation can be beneficial, ie, superior to intensive medical therapy, in achieving certain objectives. The operation is clearly a technical success, and prospects for very long-range patency of the grafts appear to be good. An observation of great interest is symptomatic improvement in some patients with occluded grafts. The greatest success of aortocoronary bypass is improvement in quality of life for certain patients.", "contents": "Aortocoronary bypass surgery for ischemic heart disease: indications based on controlled trials. One- and two-year results of aortocoronary bypass for ischemic heart disease indicate that in stable angina pectoris, at least, the operation can be beneficial, ie, superior to intensive medical therapy, in achieving certain objectives. The operation is clearly a technical success, and prospects for very long-range patency of the grafts appear to be good. An observation of great interest is symptomatic improvement in some patients with occluded grafts. The greatest success of aortocoronary bypass is improvement in quality of life for certain patients."}
{"id": "PMID:1083516", "title": "A new look at diarrhoea, diverticulitis and 'colitis' after 25 years of clinical study.", "content": "The clinical features of fat-intolerant bowel disturbance, which have been established by study, over a period of 25 years, of bowel disturbance seen in dyspeptic and other patients, are described. Excess dietary fat intake is an etiological factor in the irritable colon syndrome, spastic colon, diverticulosis, and diverticulitis of the colon and in ulcerative and non-ulcerative colitis. All these conditions are manifestations of intolerance to excess dietary fat intake, and are controlled, and their symptoms are cured, by adherence to the fat-free diet: i.e. a diet from which is excluded any fat derived from cow's milk, from the pig, any 'store' or 'depot' fat, and any vegetable fat concentrate.", "contents": "A new look at diarrhoea, diverticulitis and 'colitis' after 25 years of clinical study. The clinical features of fat-intolerant bowel disturbance, which have been established by study, over a period of 25 years, of bowel disturbance seen in dyspeptic and other patients, are described. Excess dietary fat intake is an etiological factor in the irritable colon syndrome, spastic colon, diverticulosis, and diverticulitis of the colon and in ulcerative and non-ulcerative colitis. All these conditions are manifestations of intolerance to excess dietary fat intake, and are controlled, and their symptoms are cured, by adherence to the fat-free diet: i.e. a diet from which is excluded any fat derived from cow's milk, from the pig, any 'store' or 'depot' fat, and any vegetable fat concentrate."}
{"id": "PMID:1083513", "title": "B and T lymphocytes in viral hepatitis.", "content": "Peripheral blood lymphocytes were investigated in 20 patients with acute viral hepatitis. For assessment of B lymphocytes the rosette test was carried out using sheep erythrocytes coated with antibodies and murine complement. T lymphocytes were recognized by their ability of binding uncoated sheep erythrocytes. In 16 cases the results of EAC test in patients with viral hepatitis resembled those in healthy donors. In 3 cases a significant decrease was observed and in one case a significant rise was found in the number of cells forming EAC rosettes. The T lymphocyte count was within normal range in 19 investigated cases. The results suggest that impaired T lymphocyte reactivity to PHA in acute viral hepatitis is due to a disturbed function of T lymphocytes and not to a decrease in their number. In cases associated with significant hepatocellular damage a reduction in the number of circulating B lymphocytes was observed.", "contents": "B and T lymphocytes in viral hepatitis. Peripheral blood lymphocytes were investigated in 20 patients with acute viral hepatitis. For assessment of B lymphocytes the rosette test was carried out using sheep erythrocytes coated with antibodies and murine complement. T lymphocytes were recognized by their ability of binding uncoated sheep erythrocytes. In 16 cases the results of EAC test in patients with viral hepatitis resembled those in healthy donors. In 3 cases a significant decrease was observed and in one case a significant rise was found in the number of cells forming EAC rosettes. The T lymphocyte count was within normal range in 19 investigated cases. The results suggest that impaired T lymphocyte reactivity to PHA in acute viral hepatitis is due to a disturbed function of T lymphocytes and not to a decrease in their number. In cases associated with significant hepatocellular damage a reduction in the number of circulating B lymphocytes was observed."}
{"id": "PMID:1083520", "title": "[Effect of immunosuppressive and cytostatic factors on the immune responses of the body. Communication II. Effect of hydrocortisone on immunologically competent cells (RFC cells and lymphocytes B) in immunized animals (author's transl)].", "content": "The effect of hydrocortisone on immunologically competent cells in peripheral blood (lymphocytes B and RFC cells) and on the level of humoral antibodies has been studied in rabbits immunized twice with 10per cent sheep erythrocytes. The animals were divided into two groups. The experimental group, numbering 14 rabbits, was administered 30 mg/kg body weight of hydrocortisone intramuscularly for three consecutive days before the first and second dose of antigen; the 10 rabbits of the control group were administered only the antigen. The results obtained showed that, after immunization and in the course of the normal immune response of the body, the numbers of RFC cells and of lymphocytes B increased in peripheral blood before the level of antibodies rose (Fig. 1, 3 and 4). The results in the group of experimental rabbits showed that hydrocortisone markedly suppressed the normal immune response of the body to the antigen administered. When the data obtained in both groups studied were compared, it was found that the amount of RFC cells and of lymphocytes B in peripheral blood was manifestly smaller in the group of experimental animals treated with hydrocortisone, than in the group of control animals, both after the first and the second dose of antigen (Fig. 1, 2 and 3). The difference concerning the humoral antibodies level between the two groups, however, appeared only after the second dose of the antigen (Fig. 4, Table I). That means that the investigation of immunologically competent cells in peripheral blood, such as lymphocytes B and RFC cells, enables to detect disturbances in the immune response of the body earlier than the investigation of humoral antibodies and supplies a more complete image of the changes occurring in the body during immunization.", "contents": "[Effect of immunosuppressive and cytostatic factors on the immune responses of the body. Communication II. Effect of hydrocortisone on immunologically competent cells (RFC cells and lymphocytes B) in immunized animals (author's transl)]. The effect of hydrocortisone on immunologically competent cells in peripheral blood (lymphocytes B and RFC cells) and on the level of humoral antibodies has been studied in rabbits immunized twice with 10per cent sheep erythrocytes. The animals were divided into two groups. The experimental group, numbering 14 rabbits, was administered 30 mg/kg body weight of hydrocortisone intramuscularly for three consecutive days before the first and second dose of antigen; the 10 rabbits of the control group were administered only the antigen. The results obtained showed that, after immunization and in the course of the normal immune response of the body, the numbers of RFC cells and of lymphocytes B increased in peripheral blood before the level of antibodies rose (Fig. 1, 3 and 4). The results in the group of experimental rabbits showed that hydrocortisone markedly suppressed the normal immune response of the body to the antigen administered. When the data obtained in both groups studied were compared, it was found that the amount of RFC cells and of lymphocytes B in peripheral blood was manifestly smaller in the group of experimental animals treated with hydrocortisone, than in the group of control animals, both after the first and the second dose of antigen (Fig. 1, 2 and 3). The difference concerning the humoral antibodies level between the two groups, however, appeared only after the second dose of the antigen (Fig. 4, Table I). That means that the investigation of immunologically competent cells in peripheral blood, such as lymphocytes B and RFC cells, enables to detect disturbances in the immune response of the body earlier than the investigation of humoral antibodies and supplies a more complete image of the changes occurring in the body during immunization."}
{"id": "PMID:1083525", "title": "Distribution of Ia-like molecules on the surface of normal and leukemic human cells.", "content": "Antiserum to a glycoprotein antigen complex of 23,000 and 30,000 dalton subunits (p23,30), isolated and purified from a human lymphoblastoid B cell line, was shown to be highly specific for human bursal-equivalent-processed (B) cells, reactive with 15-20% of human Null cells, but completely unreactive with human thymus-processed (T) cells. The p23,30 antigen is widely distributed on chronic lymphatic leukemic cells, 85% of acute lymphatic leukemic cells, all acute myelogenous leukemic cells, but not on chronic myelogenous leukemic cells. A rabbit antiserum specific for normal human thymocytes has also been prepared; it is reactive only with precisely that subset of acute lymphatic leukemic cells (15%) whose members do not have p23,30 on their surfaces.", "contents": "Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Antiserum to a glycoprotein antigen complex of 23,000 and 30,000 dalton subunits (p23,30), isolated and purified from a human lymphoblastoid B cell line, was shown to be highly specific for human bursal-equivalent-processed (B) cells, reactive with 15-20% of human Null cells, but completely unreactive with human thymus-processed (T) cells. The p23,30 antigen is widely distributed on chronic lymphatic leukemic cells, 85% of acute lymphatic leukemic cells, all acute myelogenous leukemic cells, but not on chronic myelogenous leukemic cells. A rabbit antiserum specific for normal human thymocytes has also been prepared; it is reactive only with precisely that subset of acute lymphatic leukemic cells (15%) whose members do not have p23,30 on their surfaces."}
{"id": "PMID:1083526", "title": "Antigen stimulation of prostaglandin synthesis and control of immune responses.", "content": "Within 2 min following the intravenous injection of sheep erythrocytes (sRBC) there occurs 20 to 80-fold increase in prostaglandin (PG) F2alpha in the spleen. This burst of synthesis is followed by a slow decline to control levels over the next 1-4 hr. No increase in splenic PGF2alpha levels is observed between 24 and 72 hr after injection. Injection of colloidal carbon results in a small increase, approximately 20% of the increase in PGF2alpha observed with sRBC. The early increase in splenic PGF2alpha levels stimulated by sRBC is also dependent upon thymus-derived (T) cells, since the increase is small or nonexistent in athymic mice and NZB mice. Also, the elevation of splenic PGF2alpha levels is blocked by the administration of indomethacin or Ro 20-5720, both of which block the synthesis of prostglandin. A small increase (2-fold) in PGF2alpha levels occurs in the thymus. A soluble antigen, bovine gamma globulin, stimulated a bimodal increase in splenic PGF2alpha levels, the early peak occurring at 2 hr and the later increase occurring at 48 hr. Using inhibitors of prostaglandin synthesis, it is possible to enhance the appearance of cells forming 19S antibody against sRBC, both in vivo and in vitro. Furthermore, inhibition of prostaglandin synthesis enhances DNA synthesis induced in a two-way mixed-lymphocyte reaction only in whole spleen cell cultures and not in cultures of spleen cells purified by passage over glass wool. Based on this evidence, it is proposed that the prostaglandins represent a major soluble mediator in the control of T cell-T cell interactions and also play an important part in T-B (bone-marrow derived) cell interactions.", "contents": "Antigen stimulation of prostaglandin synthesis and control of immune responses. Within 2 min following the intravenous injection of sheep erythrocytes (sRBC) there occurs 20 to 80-fold increase in prostaglandin (PG) F2alpha in the spleen. This burst of synthesis is followed by a slow decline to control levels over the next 1-4 hr. No increase in splenic PGF2alpha levels is observed between 24 and 72 hr after injection. Injection of colloidal carbon results in a small increase, approximately 20% of the increase in PGF2alpha observed with sRBC. The early increase in splenic PGF2alpha levels stimulated by sRBC is also dependent upon thymus-derived (T) cells, since the increase is small or nonexistent in athymic mice and NZB mice. Also, the elevation of splenic PGF2alpha levels is blocked by the administration of indomethacin or Ro 20-5720, both of which block the synthesis of prostglandin. A small increase (2-fold) in PGF2alpha levels occurs in the thymus. A soluble antigen, bovine gamma globulin, stimulated a bimodal increase in splenic PGF2alpha levels, the early peak occurring at 2 hr and the later increase occurring at 48 hr. Using inhibitors of prostaglandin synthesis, it is possible to enhance the appearance of cells forming 19S antibody against sRBC, both in vivo and in vitro. Furthermore, inhibition of prostaglandin synthesis enhances DNA synthesis induced in a two-way mixed-lymphocyte reaction only in whole spleen cell cultures and not in cultures of spleen cells purified by passage over glass wool. Based on this evidence, it is proposed that the prostaglandins represent a major soluble mediator in the control of T cell-T cell interactions and also play an important part in T-B (bone-marrow derived) cell interactions."}
{"id": "PMID:1083527", "title": "Molecular abnormality of human alpha1-antitrypsin variant (Pi-ZZ) associated with plasma activity deficiency.", "content": "A human alpha1-antitrypsin variant protein was purified to homogeneity from homozygous variant subjects (Pi-ZZ) who had a deficiency of plasma trypsin inhibitory capacity. Molecular weight, specific trypsin inhibitory capacity, and immunologic activity of the variant protein were identical to those of normal. Amino acids, N-acetylglucosamine, and hexose contents were closely similar in the normal and variant proteins, but the sialic acid content in the variant protein was significantly lower than normal. The structural difference between the normal and the variant alpha1-antitrypsin was elucidated by fingerprinting of their tryptic peptides. Two amino acid substitutions, i.e., glutamic acid in the normal protein to lysine in the variant protein, and glutamic acid in the normal protein to glutamine in the variant protein, were found.", "contents": "Molecular abnormality of human alpha1-antitrypsin variant (Pi-ZZ) associated with plasma activity deficiency. A human alpha1-antitrypsin variant protein was purified to homogeneity from homozygous variant subjects (Pi-ZZ) who had a deficiency of plasma trypsin inhibitory capacity. Molecular weight, specific trypsin inhibitory capacity, and immunologic activity of the variant protein were identical to those of normal. Amino acids, N-acetylglucosamine, and hexose contents were closely similar in the normal and variant proteins, but the sialic acid content in the variant protein was significantly lower than normal. The structural difference between the normal and the variant alpha1-antitrypsin was elucidated by fingerprinting of their tryptic peptides. Two amino acid substitutions, i.e., glutamic acid in the normal protein to lysine in the variant protein, and glutamic acid in the normal protein to glutamine in the variant protein, were found."}
{"id": "PMID:1083528", "title": "M\u00fcller cell function during spreading depression in frog retina.", "content": "K+ potentials were investigated using K+ microelectrodes in frog (Rana pipiens) retinas conditioned for spreading depression (SD) by Cl--free Ringer's solution. A marked increase in outside K+ concentration, [K+]O, maximal in the inner plexiform layer, was observed during SD. This [K+]o change resembled the simultaneously recorded membrane potential change in M\u00fcller cells, suggesting that these cells act as K+ electrodes. Intracellular recording with K+ electrodes in M\u00fcller cells showed that upon SD the [K+]i (inside) of M\u00fcller cells increases and therefore immediately starts to cleanse the extracellular space of the excess of K+ which, evidence suggests, is mainly caused by pathologically enhanced synaptic activity in the inner plexiform layer.", "contents": "M\u00fcller cell function during spreading depression in frog retina. K+ potentials were investigated using K+ microelectrodes in frog (Rana pipiens) retinas conditioned for spreading depression (SD) by Cl--free Ringer's solution. A marked increase in outside K+ concentration, [K+]O, maximal in the inner plexiform layer, was observed during SD. This [K+]o change resembled the simultaneously recorded membrane potential change in M\u00fcller cells, suggesting that these cells act as K+ electrodes. Intracellular recording with K+ electrodes in M\u00fcller cells showed that upon SD the [K+]i (inside) of M\u00fcller cells increases and therefore immediately starts to cleanse the extracellular space of the excess of K+ which, evidence suggests, is mainly caused by pathologically enhanced synaptic activity in the inner plexiform layer."}
{"id": "PMID:1083533", "title": "Stimulation of antibody synthesizing lymphocytes in the rat by antigen reactive cells sensitized in vitro.", "content": "Lymphocytes obtained from rat spleens were sensitized in vitro to sheep erythrocytes. The sensitized lymphocytes did not synthesize antibody but when injected into unimmunized rats did stimulate antibody production to sheep erythrocytes. Analysis of the location of 7 and 19 S plaque-forming cells in the recipient rats indicated that these two antibody synthesizing cell populations arise from different lymphocytes. It was also found that the sensitized lymphocytes could react only with a limited number of antibody synthesizing cells.", "contents": "Stimulation of antibody synthesizing lymphocytes in the rat by antigen reactive cells sensitized in vitro. Lymphocytes obtained from rat spleens were sensitized in vitro to sheep erythrocytes. The sensitized lymphocytes did not synthesize antibody but when injected into unimmunized rats did stimulate antibody production to sheep erythrocytes. Analysis of the location of 7 and 19 S plaque-forming cells in the recipient rats indicated that these two antibody synthesizing cell populations arise from different lymphocytes. It was also found that the sensitized lymphocytes could react only with a limited number of antibody synthesizing cells."}
{"id": "PMID:1083534", "title": "Sugar-induced potential difference and short circuit current in bullfrog small intestine: effect of UO22+.", "content": "UO22+ 1.3 mM added as UO2(NO3)2 to the mucosal solution consistently inhibited the P.D. and ISC evoked by 11 mM glucose and 35 mM 3-O-methyl glucose across isolated strips of bullfrog small intestine bathed by symmetrical Ringer solutions in which SO42- was the major anion. The average degree of inhibition in the presence of glucose was 42 +/- 7(SEM) percent. P.D. and ISC in the absence of transported solutes were not significatnly altered by mucosal UO22+ at this concentration. Increasing the mucosal UO22+ concentration to 2.6 mM did not significantly increase its inhibitory action on glucose-evoked P.D. and ISC. Further increasing the UO22+ concentration to 13 mM completely inhibited glucose-induced P.D. and ISC but also markedly reduced these parameters in the absence of glucose. Serosal UO22+ (1.3mM) had no effect on the P.D. and ISC evoked by glucose and 3-O-methyl glucose. It is suggested that the inhibitory action of UO22+ involves binding of this ion to anionic sites located in the apical membrane of the absorptive cells. Mucosal or serosal UO22+ (1.3 mM) had no effect on the P.D. and ISC elicited by 20 mM valine, indicating that under the conditions of these experiments UO22+ selectively inhibits sugar-induced P.D. and ISC and, by implication mucosal sugar uptake.", "contents": "Sugar-induced potential difference and short circuit current in bullfrog small intestine: effect of UO22+. UO22+ 1.3 mM added as UO2(NO3)2 to the mucosal solution consistently inhibited the P.D. and ISC evoked by 11 mM glucose and 35 mM 3-O-methyl glucose across isolated strips of bullfrog small intestine bathed by symmetrical Ringer solutions in which SO42- was the major anion. The average degree of inhibition in the presence of glucose was 42 +/- 7(SEM) percent. P.D. and ISC in the absence of transported solutes were not significatnly altered by mucosal UO22+ at this concentration. Increasing the mucosal UO22+ concentration to 2.6 mM did not significantly increase its inhibitory action on glucose-evoked P.D. and ISC. Further increasing the UO22+ concentration to 13 mM completely inhibited glucose-induced P.D. and ISC but also markedly reduced these parameters in the absence of glucose. Serosal UO22+ (1.3mM) had no effect on the P.D. and ISC evoked by glucose and 3-O-methyl glucose. It is suggested that the inhibitory action of UO22+ involves binding of this ion to anionic sites located in the apical membrane of the absorptive cells. Mucosal or serosal UO22+ (1.3 mM) had no effect on the P.D. and ISC elicited by 20 mM valine, indicating that under the conditions of these experiments UO22+ selectively inhibits sugar-induced P.D. and ISC and, by implication mucosal sugar uptake."}
{"id": "PMID:1083535", "title": "Testosterone manipulations: effects on ranacide aggression and brain monoamines in the adult female rat.", "content": "The effect of testosterone propionate on ranacide (frog-killing) behavior and brain norepinephrine (NE), dopamine (DA) and serotonin (5-HT) levels was determined in 40 female Wistar rats. Adult rats were screened for frog killing behavior on the basis of a single 30 min testing session. Aggressors were defined as animals which attacked or killed during this session while nonaggressors failed to do so. Using either aggressors or nonaggressors, testosterone and sesame oil equally increased aggresive behavior as measured in a second 30 min testing session. Biochemical analyses indicated that testosterone treated animals had significantly higher brain NE and NE/5-HT levels. Aggressors, testosterone or sesame treated had higher NE/5-HT ratios. Whole-brain levels of DA and 5-HT and the DA/5-HT ratios were unaffected. It is concluded that the elicitation of ranacide in the adult female rat is not androgen dependent nor is this behavior functionally related to the observed differences in brain noradrenergic/serotonergic levels. This study provides additional evidence that ranacide is a type of predatory aggression and yet presents data which may be at variance with the classic monoaminergic theory of aggressive behaviors.", "contents": "Testosterone manipulations: effects on ranacide aggression and brain monoamines in the adult female rat. The effect of testosterone propionate on ranacide (frog-killing) behavior and brain norepinephrine (NE), dopamine (DA) and serotonin (5-HT) levels was determined in 40 female Wistar rats. Adult rats were screened for frog killing behavior on the basis of a single 30 min testing session. Aggressors were defined as animals which attacked or killed during this session while nonaggressors failed to do so. Using either aggressors or nonaggressors, testosterone and sesame oil equally increased aggresive behavior as measured in a second 30 min testing session. Biochemical analyses indicated that testosterone treated animals had significantly higher brain NE and NE/5-HT levels. Aggressors, testosterone or sesame treated had higher NE/5-HT ratios. Whole-brain levels of DA and 5-HT and the DA/5-HT ratios were unaffected. It is concluded that the elicitation of ranacide in the adult female rat is not androgen dependent nor is this behavior functionally related to the observed differences in brain noradrenergic/serotonergic levels. This study provides additional evidence that ranacide is a type of predatory aggression and yet presents data which may be at variance with the classic monoaminergic theory of aggressive behaviors."}
{"id": "PMID:1083536", "title": "Time-dependent results of amino acid uptake studies of learning in frogs.", "content": "Myelencephalic grass frogs were trained to elevate the right forelimb to avoid a shock plate at several times in relation to administration of 3H-leucine and sacrifice. Total radioactivity (excluding 3H2O) and radioactivity in the soluble pool were significantly greater in the trained animals. The differences between trained and yoked animals decreased with increasing time, up to 30 min after training. The opposite trend was apparent in TCA insoluble material; differences between trained and yoked animals increased with increasing time after training. Similar experiments with 3H-inulin provide evidence that the effects were not due to generalized permeability increases or circulatory alterations.", "contents": "Time-dependent results of amino acid uptake studies of learning in frogs. Myelencephalic grass frogs were trained to elevate the right forelimb to avoid a shock plate at several times in relation to administration of 3H-leucine and sacrifice. Total radioactivity (excluding 3H2O) and radioactivity in the soluble pool were significantly greater in the trained animals. The differences between trained and yoked animals decreased with increasing time, up to 30 min after training. The opposite trend was apparent in TCA insoluble material; differences between trained and yoked animals increased with increasing time after training. Similar experiments with 3H-inulin provide evidence that the effects were not due to generalized permeability increases or circulatory alterations."}
{"id": "PMID:1083537", "title": "Simultaneous efflux of K+ and Na+ from frog sartorius muscle freed of extracellular fluids: evidence for rapidly exchanging Na+ from the cells.", "content": "After removal of radioactivity trapped in the extracellular space and correcting for the contribution of connective tissue elements, the K+ -efflux curve of frog sartorius muscles becomes a perfect straight line in a semilogarithmic plot. The simultaneously recorded Na+-efflux curve from the same muscles remains strongly curved, and can be resolved into a slow fraction (which conventionally has been regarded as representing the entire cell Na+) and at least one fast fraction. The fast fraction of Na+ could not have originated from a sarcoplasmic reticulum or any other extracellular space extensions; otherwise a similar fast fraction should exist for K+. The data agree with the interpretation that it is the fast fraction that is rate limiting by cell permeability and the slow fraction by desorption from intracellular adsorption sites.", "contents": "Simultaneous efflux of K+ and Na+ from frog sartorius muscle freed of extracellular fluids: evidence for rapidly exchanging Na+ from the cells. After removal of radioactivity trapped in the extracellular space and correcting for the contribution of connective tissue elements, the K+ -efflux curve of frog sartorius muscles becomes a perfect straight line in a semilogarithmic plot. The simultaneously recorded Na+-efflux curve from the same muscles remains strongly curved, and can be resolved into a slow fraction (which conventionally has been regarded as representing the entire cell Na+) and at least one fast fraction. The fast fraction of Na+ could not have originated from a sarcoplasmic reticulum or any other extracellular space extensions; otherwise a similar fast fraction should exist for K+. The data agree with the interpretation that it is the fast fraction that is rate limiting by cell permeability and the slow fraction by desorption from intracellular adsorption sites."}
{"id": "PMID:1083538", "title": "Intracellular resistance and conduction in bullfrog atrium.", "content": "The present study was undertaken to study the mechanism of transmission across the junctions between cells of cardiac muscle. Two cases, one with high resistance junction and the second with low resistance junctions, were modeled mathematically and each model was analyzed to predict the ratio of the transverse to longitudinal threshold field strengths. This ratio was measured experimentally for atrial trabeculae from Rana catesbiana and agreed well with the value predicted by the model in which the resistance of the membranes at the junctions is low. It can be inferred, therefore, that the resistance at the junctions between cells of bullfrog atrial muscle is low enough to permit the flow of sufficient current from the pre- to postjunctional cell to cause excitation of the latter. One parameter required in the analysis was the space constant, lambda. This was measured with the trabecula in a volume conductor using a large planar external electrode to produce a change in the transmembrane potential and a microelectrode to measure the spatial decrement of the electrotonic potential. The measurements of electrotonic decrement in 16 trabeculae are pooled to provide an estimate of the value of the space constant in frog atrial trabeculae: lambda= 0.863 +/- 0.041 mm, s.d., n=96.", "contents": "Intracellular resistance and conduction in bullfrog atrium. The present study was undertaken to study the mechanism of transmission across the junctions between cells of cardiac muscle. Two cases, one with high resistance junction and the second with low resistance junctions, were modeled mathematically and each model was analyzed to predict the ratio of the transverse to longitudinal threshold field strengths. This ratio was measured experimentally for atrial trabeculae from Rana catesbiana and agreed well with the value predicted by the model in which the resistance of the membranes at the junctions is low. It can be inferred, therefore, that the resistance at the junctions between cells of bullfrog atrial muscle is low enough to permit the flow of sufficient current from the pre- to postjunctional cell to cause excitation of the latter. One parameter required in the analysis was the space constant, lambda. This was measured with the trabecula in a volume conductor using a large planar external electrode to produce a change in the transmembrane potential and a microelectrode to measure the spatial decrement of the electrotonic potential. The measurements of electrotonic decrement in 16 trabeculae are pooled to provide an estimate of the value of the space constant in frog atrial trabeculae: lambda= 0.863 +/- 0.041 mm, s.d., n=96."}
{"id": "PMID:1083539", "title": "The anti-inflammatory agents used in rheumatic disorders.", "content": "The objectives of nonspecific anti-inflammatory therapy are, for the most part, to relieve symptoms. In selected patients such therapy may also slow, prevent, or reverse the destructive effects of the inflammatory process. It is well to remember that none of the wide variety of anti-inflammatory agents is without some inherent toxicity, although some agents are obviously much more toxic than others. The physician prescribing these agents must keep in mind both the potential benefits and the potential risks attendant to their use. In this way he can balance potential benefit against potential risk and arrive at the most rational drug therapy for any given rheumatic disorder.", "contents": "The anti-inflammatory agents used in rheumatic disorders. The objectives of nonspecific anti-inflammatory therapy are, for the most part, to relieve symptoms. In selected patients such therapy may also slow, prevent, or reverse the destructive effects of the inflammatory process. It is well to remember that none of the wide variety of anti-inflammatory agents is without some inherent toxicity, although some agents are obviously much more toxic than others. The physician prescribing these agents must keep in mind both the potential benefits and the potential risks attendant to their use. In this way he can balance potential benefit against potential risk and arrive at the most rational drug therapy for any given rheumatic disorder."}
{"id": "PMID:1083541", "title": "[Indications for surgical and medical treatment of coronary artery disease (author's transl)].", "content": "The following effects in treatment of coronary artery disease are desired: 1. Elimination or improvement of angina. 2. Improvement of physical capacity. 3. Prevention of imminent complications (myocardial infarct, cardiac arrhythmias, heart failure, embolism). 4. Elimination or diminuation of risk factors. 5. Prolongation of life. - In a critical survey concerning long-term studies of patients with aorto-coronary bypass or medical treatment in the literature subtile lists of indications for surgical and conservative treatment are put up (Table II and III), illustrated by case reports. - Useful criteria for diagnosis, follow-up, and prognosis are selective coronary angiography, ventriculography as well as determination of the coronary reserve (Argon Method). Indication for aorto-coronary bypass and resection of myocardial aneurysms are presented. Principles of medical treatment are: 1. Diminuation of myocardial oxygen requirement (release of pressure, economisation of work load, recompensation, regulation of arrhythmias) and 2. improvement of myocardial oxygen supply (Diminuation of coronary perfusion resistance including prevention and treatment of atherosclerosis). Indication for various medications are discussed (nitrites, beta-adrenergic blocking agents and antihypertensive drugs, glycosides, medication for arrhythmias, coronary dilatators, anticoagulants, and lipotropic substances). Their mode of action is debated and documented by own results. Present possibilities and limits in treatment of coronary artery disease are presented.", "contents": "[Indications for surgical and medical treatment of coronary artery disease (author's transl)]. The following effects in treatment of coronary artery disease are desired: 1. Elimination or improvement of angina. 2. Improvement of physical capacity. 3. Prevention of imminent complications (myocardial infarct, cardiac arrhythmias, heart failure, embolism). 4. Elimination or diminuation of risk factors. 5. Prolongation of life. - In a critical survey concerning long-term studies of patients with aorto-coronary bypass or medical treatment in the literature subtile lists of indications for surgical and conservative treatment are put up (Table II and III), illustrated by case reports. - Useful criteria for diagnosis, follow-up, and prognosis are selective coronary angiography, ventriculography as well as determination of the coronary reserve (Argon Method). Indication for aorto-coronary bypass and resection of myocardial aneurysms are presented. Principles of medical treatment are: 1. Diminuation of myocardial oxygen requirement (release of pressure, economisation of work load, recompensation, regulation of arrhythmias) and 2. improvement of myocardial oxygen supply (Diminuation of coronary perfusion resistance including prevention and treatment of atherosclerosis). Indication for various medications are discussed (nitrites, beta-adrenergic blocking agents and antihypertensive drugs, glycosides, medication for arrhythmias, coronary dilatators, anticoagulants, and lipotropic substances). Their mode of action is debated and documented by own results. Present possibilities and limits in treatment of coronary artery disease are presented."}
{"id": "PMID:1083542", "title": "[Surgical treatment of occlusive coronary artery disease. Methods and results (author's transl)].", "content": "A brief history of the development of coronary heart surgery is presented. Modern methods include the aorto-coronary bypass using saphenous vein graft or the thoracic internal artery, and the resection of ventricular aneurysm. The own experience with a group of 262 patients revealed a total mortality of 11,4%. Selected indication, improved surgical technique and postoperative care changed coronary surgery during the recent years into a routine method. The operative risk is characterized by the anatomical location of the occlusive artery disease as well as by the left ventricular function. But even in the group with high surgical risk the operative treatment is justified since life expectancy under conservative therapy is limited. In a group with 17 late death the main cause was due to cardiac insufficiency. In the group of survivors approximately 84% of the bypass were patent after an average period of 18 months.", "contents": "[Surgical treatment of occlusive coronary artery disease. Methods and results (author's transl)]. A brief history of the development of coronary heart surgery is presented. Modern methods include the aorto-coronary bypass using saphenous vein graft or the thoracic internal artery, and the resection of ventricular aneurysm. The own experience with a group of 262 patients revealed a total mortality of 11,4%. Selected indication, improved surgical technique and postoperative care changed coronary surgery during the recent years into a routine method. The operative risk is characterized by the anatomical location of the occlusive artery disease as well as by the left ventricular function. But even in the group with high surgical risk the operative treatment is justified since life expectancy under conservative therapy is limited. In a group with 17 late death the main cause was due to cardiac insufficiency. In the group of survivors approximately 84% of the bypass were patent after an average period of 18 months."}
{"id": "PMID:1083543", "title": "Evaluation of pediatric hydrocephalus by computed tomography.", "content": "Computed tomography (CT) is a safe and reliable technique for the study of children with increased head circumference. Hydrocephalic children requiring drainage of cerebrospinal fluid may be shunted on the basis of the CT scan alone and their postsurgical course followed by serial CT scans thereafter. Any additional pneumographic studies required may be performed via the existing shunt tube, eliminating transcerebral catheterization and its attendant complications.", "contents": "Evaluation of pediatric hydrocephalus by computed tomography. Computed tomography (CT) is a safe and reliable technique for the study of children with increased head circumference. Hydrocephalic children requiring drainage of cerebrospinal fluid may be shunted on the basis of the CT scan alone and their postsurgical course followed by serial CT scans thereafter. Any additional pneumographic studies required may be performed via the existing shunt tube, eliminating transcerebral catheterization and its attendant complications."}
{"id": "PMID:1083544", "title": "Diagnosis of epidermoid tumor by computed tomography. Analysis and evaluation of findings.", "content": "Primary intracranial intradural extraventricular epidermoid tumor may be suggested by the findings on computed tomography (CT), which is also useful during the follow-up period. Epidermoid tumors may exhibit minimal symptoms and have usually spread far beyond their original site by the time they are found. Early detection, delineation, and characterization are possible with CT.", "contents": "Diagnosis of epidermoid tumor by computed tomography. Analysis and evaluation of findings. Primary intracranial intradural extraventricular epidermoid tumor may be suggested by the findings on computed tomography (CT), which is also useful during the follow-up period. Epidermoid tumors may exhibit minimal symptoms and have usually spread far beyond their original site by the time they are found. Early detection, delineation, and characterization are possible with CT."}
{"id": "PMID:1083545", "title": "The anterior inferior cerebellar artery in mass lesions. Preliminary findings with emphasis on the lateral projection.", "content": "Analysis of abnormalities of the configuration of the anterior inferior cerebellar artery in lateral projection facilitates diagnosis of posterior fossa masses. Cerebellopontomedullary angle lesions frequently cause arcuate posterior-inferior displacements of the rostrolateral artery or reversal of curvature of the ascending segment of the meatal loop. Vermian, hemispheric, intra-fourth ventricular, and suitably situated extra-axial masses alter the plane of the lateral loop of the caudomedial artery. Tonsillar herniation may be detected in patients with hypoplastic or absent posterior inferior cerebellar arteries by analyzing the relationship of the biventral segment of the descending or caudomedial artery to the foramen magnum.", "contents": "The anterior inferior cerebellar artery in mass lesions. Preliminary findings with emphasis on the lateral projection. Analysis of abnormalities of the configuration of the anterior inferior cerebellar artery in lateral projection facilitates diagnosis of posterior fossa masses. Cerebellopontomedullary angle lesions frequently cause arcuate posterior-inferior displacements of the rostrolateral artery or reversal of curvature of the ascending segment of the meatal loop. Vermian, hemispheric, intra-fourth ventricular, and suitably situated extra-axial masses alter the plane of the lateral loop of the caudomedial artery. Tonsillar herniation may be detected in patients with hypoplastic or absent posterior inferior cerebellar arteries by analyzing the relationship of the biventral segment of the descending or caudomedial artery to the foramen magnum."}
{"id": "PMID:1083546", "title": "Abnormal pulmonary gallium accumulation in P. carinii pneumonia.", "content": "The potentially fatal interstitial pneumonia of Pneumocystis carinii is a frequent opportunistic invader of patients treated for malignancy with immunosuppressive and cytotoxic agents. Generalized Ga-67 pulmonary localization which is markedly disproportionate to the clinical and radiographic findings has led to earlier diagnoses by open lung biopsy in this setting. The potential usefulness of gallium scintigraphy in patients with suspected P. Carini pneumonia is presented.", "contents": "Abnormal pulmonary gallium accumulation in P. carinii pneumonia. The potentially fatal interstitial pneumonia of Pneumocystis carinii is a frequent opportunistic invader of patients treated for malignancy with immunosuppressive and cytotoxic agents. Generalized Ga-67 pulmonary localization which is markedly disproportionate to the clinical and radiographic findings has led to earlier diagnoses by open lung biopsy in this setting. The potential usefulness of gallium scintigraphy in patients with suspected P. Carini pneumonia is presented."}
{"id": "PMID:1083548", "title": "Alterations in the cellular-mediated immune responsiveness of chronic marihuana smokers.", "content": "Chronic marihuana smokers and matched nonsmokers were compared with respect to several aspects of both their humoral and cellular immune system. Immunoglobulin and complement levels, SMA 12 and hematologic values from marihuana smokers did not differ significantly from those obtained from nonsmokers. However, the number of T-lymphocytes with respect to the ratio of T and B-lymphocytes in the peripheral blood was lowered in marihuana smokers. In addition, phytohemagglutinin stimulation of lymphocytes was apparently less effective in smokers. The polymorphonuclear leukocytes (PNM) from the blood of smokers contained fewer cells capable of phagocytizing yeast cells than did PMN from nonsmokers. While marihuana smoking does appear to affect immune mechanisms at no time in these studies were any deleterious physiological effects that could be directly associated with the alterations in the immune system observed in the marihuana smokers.", "contents": "Alterations in the cellular-mediated immune responsiveness of chronic marihuana smokers. Chronic marihuana smokers and matched nonsmokers were compared with respect to several aspects of both their humoral and cellular immune system. Immunoglobulin and complement levels, SMA 12 and hematologic values from marihuana smokers did not differ significantly from those obtained from nonsmokers. However, the number of T-lymphocytes with respect to the ratio of T and B-lymphocytes in the peripheral blood was lowered in marihuana smokers. In addition, phytohemagglutinin stimulation of lymphocytes was apparently less effective in smokers. The polymorphonuclear leukocytes (PNM) from the blood of smokers contained fewer cells capable of phagocytizing yeast cells than did PMN from nonsmokers. While marihuana smoking does appear to affect immune mechanisms at no time in these studies were any deleterious physiological effects that could be directly associated with the alterations in the immune system observed in the marihuana smokers."}
{"id": "PMID:1083553", "title": "[The salivary glands during rheumatoid arthritis].", "content": "Salivary gland lesions were studied in 65 non-selected, confirmed rheumatoid polyarthritis patients. The investigations included a careful clinical study, the Schirmer test, sialography, sequential scintigraphy with Technetium 99, and a histological study of the sublingual glands. Tests were also made for salivary gland anti-epithelium antibodies. The frequency of sialography and scintigraphy anomalies is emphasized. In different degrees, two of of three patients presented anatomical or functional anomalies of the salivary glands. These disturbances were not related to the duration, the functional severity, or the treatment of the disease or to the presence of anti-salivary gland antibodies. On the other hand, there was a clear relation with both the presence of anti-nuclear antibodies and an increase in the serum gammaglobulin levels. The limits of the Gougerot syndrome are discussed and it is proposed that there is a need for a second nosological class grouping the minor scintigraphic and sialographic anomalies related to rheumatoid arthritis that has been suggested by several authors.", "contents": "[The salivary glands during rheumatoid arthritis]. Salivary gland lesions were studied in 65 non-selected, confirmed rheumatoid polyarthritis patients. The investigations included a careful clinical study, the Schirmer test, sialography, sequential scintigraphy with Technetium 99, and a histological study of the sublingual glands. Tests were also made for salivary gland anti-epithelium antibodies. The frequency of sialography and scintigraphy anomalies is emphasized. In different degrees, two of of three patients presented anatomical or functional anomalies of the salivary glands. These disturbances were not related to the duration, the functional severity, or the treatment of the disease or to the presence of anti-salivary gland antibodies. On the other hand, there was a clear relation with both the presence of anti-nuclear antibodies and an increase in the serum gammaglobulin levels. The limits of the Gougerot syndrome are discussed and it is proposed that there is a need for a second nosological class grouping the minor scintigraphic and sialographic anomalies related to rheumatoid arthritis that has been suggested by several authors."}
{"id": "PMID:1083554", "title": "[Intermittent rheumatism revealing a familial syndrome. Arthritis--urticarian eruptions--deafness: Muckle-Wells syndrome without kidney amylosis].", "content": "At a consultation with a 31-year-old man, motivated by painful episodes of joint pain that had started considerably earlier, a familial disease entity was discovered that included the three clinical signs of the Muckle and Wells syndrome : urticarial eruption, intermittent pain in the limbs originating in the joints, and bilateral deafness of perception. In spite of the absnece of renal amylosis, the similarity of the characteristics of these three elements, as well as the nature of the biochemical disturbances, and the mode of transmission, led the authors to consider these observations in the contest of this syndrome. The joint manifestations being indicative, the signs of this intermittent rheumatism are described, as well as the characteristics of the other disorders.", "contents": "[Intermittent rheumatism revealing a familial syndrome. Arthritis--urticarian eruptions--deafness: Muckle-Wells syndrome without kidney amylosis]. At a consultation with a 31-year-old man, motivated by painful episodes of joint pain that had started considerably earlier, a familial disease entity was discovered that included the three clinical signs of the Muckle and Wells syndrome : urticarial eruption, intermittent pain in the limbs originating in the joints, and bilateral deafness of perception. In spite of the absnece of renal amylosis, the similarity of the characteristics of these three elements, as well as the nature of the biochemical disturbances, and the mode of transmission, led the authors to consider these observations in the contest of this syndrome. The joint manifestations being indicative, the signs of this intermittent rheumatism are described, as well as the characteristics of the other disorders."}
{"id": "PMID:1083555", "title": "Genetic control of B-cell responses. II. Identification of the spleen B-cell defect in C3H/HeJ mice.", "content": "The responsiveness of spleen cells from C3H/HeJ and C3H/Tif mice to lipopolysaccharide (LPS) was compared. Around 1,000-fold higher concentration of LPS were required to minimally activate HeJ cells, as compared with Tif high-responder cells, to both proliferation and polyclonal antibody secretion. However, HeJ cells did respond to higher LPS concentrations (100 and 1000 mug/ml). This selective pattern of responsiveness to LPS was also observed in the polyclonal responses of strains to LPS in vivo. Furthermore, the unresponsiveness of HeJ spleen cells was found to depend on a pure B-cell defect in the capacity to interact and/or generate triggering signals on interaction with LPS. Thus, adherent cells, thymus-derived lymphocytes, serum factors, and other non-specific conditions inherent in spleen cell suspensions of low-responder mice were not responsible for suppressing a putative B-cell response to LPS. The present findings are compatible with the possibility that the defect in C3H/HeJ B cells reflects the absence of a structure on the cell surface membrane that is functionally responsible for mediating LPS triggering.", "contents": "Genetic control of B-cell responses. II. Identification of the spleen B-cell defect in C3H/HeJ mice. The responsiveness of spleen cells from C3H/HeJ and C3H/Tif mice to lipopolysaccharide (LPS) was compared. Around 1,000-fold higher concentration of LPS were required to minimally activate HeJ cells, as compared with Tif high-responder cells, to both proliferation and polyclonal antibody secretion. However, HeJ cells did respond to higher LPS concentrations (100 and 1000 mug/ml). This selective pattern of responsiveness to LPS was also observed in the polyclonal responses of strains to LPS in vivo. Furthermore, the unresponsiveness of HeJ spleen cells was found to depend on a pure B-cell defect in the capacity to interact and/or generate triggering signals on interaction with LPS. Thus, adherent cells, thymus-derived lymphocytes, serum factors, and other non-specific conditions inherent in spleen cell suspensions of low-responder mice were not responsible for suppressing a putative B-cell response to LPS. The present findings are compatible with the possibility that the defect in C3H/HeJ B cells reflects the absence of a structure on the cell surface membrane that is functionally responsible for mediating LPS triggering."}
{"id": "PMID:1083556", "title": "Demonstration of T lymphocytes in cerebrospinal fluid.", "content": "Cerebrospinal fluid (CSF) was allowed to drop straight into Hanks's balanced salt solution. After centrifugation the pellet was resuspended and mixed with sheep erythrocytes. The mixture was further handled as in the E-rosette test with peripheral blood lymphocytes. CSF from 20 individuals were investigated, and rosette-forming cells (RFC) were found in all. Six patients with normal fluid had between 46% and 83% RFC. Four patients with multiple sclerosis had increased numbers of RFC (94%-96%). Low numbers of RFC were found in one patient with cerebellar ataxia and in one of two patients with acute viral meningitis. With this technique RFC can be counted even in normal CSF with a 3-ml sample.", "contents": "Demonstration of T lymphocytes in cerebrospinal fluid. Cerebrospinal fluid (CSF) was allowed to drop straight into Hanks's balanced salt solution. After centrifugation the pellet was resuspended and mixed with sheep erythrocytes. The mixture was further handled as in the E-rosette test with peripheral blood lymphocytes. CSF from 20 individuals were investigated, and rosette-forming cells (RFC) were found in all. Six patients with normal fluid had between 46% and 83% RFC. Four patients with multiple sclerosis had increased numbers of RFC (94%-96%). Low numbers of RFC were found in one patient with cerebellar ataxia and in one of two patients with acute viral meningitis. With this technique RFC can be counted even in normal CSF with a 3-ml sample."}
{"id": "PMID:1083557", "title": "Amyloid-related serum protein (SAA) in patients with inherited amyloidosis and certain viral conditions.", "content": "Amyloid-related serum protein (SAA) was studied with a double immunodiffusion method. Only 2 of 21 patients with inherited systemic amyloidosis and lattice corneal dystrophy had elevated SAA levels in their sera. Several pseudoexfoliation patients had elevated levels of SAA, but it was not possible to differentiate an age-related elevation of SAA from that associated with this disease. In addition, SAA was found in the sera of many groups of patients with acute viral infections, including 10 of 11 patients with measles and 7 of 11 persons vaccinated against rubella. Elevation of SAA protein appeared in the serum before the 6th day after the vaccination, had its peak value between days 6 and 12, but was absent already on the 20th day. In this relation the SAA protein appears to behave as an acute-phase reactant.", "contents": "Amyloid-related serum protein (SAA) in patients with inherited amyloidosis and certain viral conditions. Amyloid-related serum protein (SAA) was studied with a double immunodiffusion method. Only 2 of 21 patients with inherited systemic amyloidosis and lattice corneal dystrophy had elevated SAA levels in their sera. Several pseudoexfoliation patients had elevated levels of SAA, but it was not possible to differentiate an age-related elevation of SAA from that associated with this disease. In addition, SAA was found in the sera of many groups of patients with acute viral infections, including 10 of 11 patients with measles and 7 of 11 persons vaccinated against rubella. Elevation of SAA protein appeared in the serum before the 6th day after the vaccination, had its peak value between days 6 and 12, but was absent already on the 20th day. In this relation the SAA protein appears to behave as an acute-phase reactant."}
{"id": "PMID:1083558", "title": "Mitogenic properties of polyene antibiotics for murine B cells.", "content": "Two polyene antibiotics, nystatin and amphotericin B, were found to be mitogenic for mouse spleen cells as measured by induction of DNA synthesis and polyclonal antibody production. This effect was demonstrated on spleen cells from nude mice and anti-theta-treated spleen cells from normal mice. No effect was found on cortisone-resistant thymocytes or on spleen cells treated with anti-mouse bone marrow-derived lymphocyte antigen antiserum. Nor was there any effect on spleen cells passed through a nylon fiber column. Thus we conclude that nystatin and amphotericin B are murine B-cell mitogens.", "contents": "Mitogenic properties of polyene antibiotics for murine B cells. Two polyene antibiotics, nystatin and amphotericin B, were found to be mitogenic for mouse spleen cells as measured by induction of DNA synthesis and polyclonal antibody production. This effect was demonstrated on spleen cells from nude mice and anti-theta-treated spleen cells from normal mice. No effect was found on cortisone-resistant thymocytes or on spleen cells treated with anti-mouse bone marrow-derived lymphocyte antigen antiserum. Nor was there any effect on spleen cells passed through a nylon fiber column. Thus we conclude that nystatin and amphotericin B are murine B-cell mitogens."}
{"id": "PMID:1083559", "title": "Heterogeneity of B cells: direct evidence of selective triggering of distinct subpopulations by polyclonal activators.", "content": "This investigation was undertaken to assess directly the previous postulate that distinct subsets of B cells in the adult mouse spleen are selectivity triggered by different polyclonal B-cell activators (PBA). Several strains of mice and lipopolysaccharide (LPS), purified protein derivative of tuberculin (PPD), and dextran sulphate (DS) were used in this study. Two experimental approaches were used: stimulation after addition of two PBAs simultaneously to cell cultures and eliminating the responding population to one PBA, by a hot pulse of radioactive thymidine, on a later response to another PBA. The results of these experiments indicated that DS stimulated a cell population completely different from that stimulated by the LPS- and PPD-sensitive cells. When LPS and PPD stimulations were compared, it was found that the cells responding to these PBAs were largely distinct, although some cells were sensitive to both these PBAs. The extension of the overlaps in these subsets (the number of cells that could be activated by either PBA) was found to vary from one strain to another. These experiments gave direct evidence of the existence of subsets that can be activated by different PBAs. The present results also provided indications of the functional performance of distinct subsets of B cells on activation.", "contents": "Heterogeneity of B cells: direct evidence of selective triggering of distinct subpopulations by polyclonal activators. This investigation was undertaken to assess directly the previous postulate that distinct subsets of B cells in the adult mouse spleen are selectivity triggered by different polyclonal B-cell activators (PBA). Several strains of mice and lipopolysaccharide (LPS), purified protein derivative of tuberculin (PPD), and dextran sulphate (DS) were used in this study. Two experimental approaches were used: stimulation after addition of two PBAs simultaneously to cell cultures and eliminating the responding population to one PBA, by a hot pulse of radioactive thymidine, on a later response to another PBA. The results of these experiments indicated that DS stimulated a cell population completely different from that stimulated by the LPS- and PPD-sensitive cells. When LPS and PPD stimulations were compared, it was found that the cells responding to these PBAs were largely distinct, although some cells were sensitive to both these PBAs. The extension of the overlaps in these subsets (the number of cells that could be activated by either PBA) was found to vary from one strain to another. These experiments gave direct evidence of the existence of subsets that can be activated by different PBAs. The present results also provided indications of the functional performance of distinct subsets of B cells on activation."}
{"id": "PMID:1083560", "title": "Assessment of Peroheme-40 for the presence of occult blood in the faeces.", "content": "Peroheme 40, a chemical test for occult blood in faeces, has been assessed by comparing it with results obtained using 51Cr labelled red cells. The study was carried out in two parts. Initially testing was carried out on 75 stool samples from 20 patients on a restricted diet, and in the second part, testing was performed on 40 samples from 10 patients on a normal ward diet. All patients were suspected of losing blood from the gastrointestinal tract. On the restricted diet there were 29 per cent false positive and 13 per cent false negative results, while on the normal diet, there were 86 per cent false positive and 8 per cent false negative results. Peroheme-40 has therefore been shown, on a restricted diet, to compare favourably with previously available chemical tests.", "contents": "Assessment of Peroheme-40 for the presence of occult blood in the faeces. Peroheme 40, a chemical test for occult blood in faeces, has been assessed by comparing it with results obtained using 51Cr labelled red cells. The study was carried out in two parts. Initially testing was carried out on 75 stool samples from 20 patients on a restricted diet, and in the second part, testing was performed on 40 samples from 10 patients on a normal ward diet. All patients were suspected of losing blood from the gastrointestinal tract. On the restricted diet there were 29 per cent false positive and 13 per cent false negative results, while on the normal diet, there were 86 per cent false positive and 8 per cent false negative results. Peroheme-40 has therefore been shown, on a restricted diet, to compare favourably with previously available chemical tests."}
{"id": "PMID:1083563", "title": "Quantitative evaluation of left ventricular bypass in reducing myocardial ischemia.", "content": "A controlled study was undertaken to evaluate the relationship between myocardial oxygen consumption (MVO2) and epicardial S-T segment improvement in an ischemic heart preparation during left ventricular (LV) bypass. Twelve mongrel dogs were prepared with left ventricular-aortic bypass, coronary sinus cannulation, and a multiple point epicardial electrocardiographic technique. The left anterior descending coronary artery was ligated low (Group I) or high (Group II) after baseline studies. After ischemic baseline studies, increasing degrees of LV bypass were performed at a constant mean aortic pressure and heart rate. The final infarct size was determined by the nitroblue tetrazolium staining technique. Group I--a significant reduction in the average (S-T) segment elevation occurred during 50 to 59 percent of LV bypass during which MVO2 was reduced 10.9 percent from controls. A maximum S-T reduction of 75 percent occurred during LV decompression during which MVO2 was reduced 52.8 percent from controls. The final infarct size for Group I was 9.8 +/- 0.9 Gm. per 100 Gm. of LV. Group II--a significant reduction in S-T segment elevation occurred only after 90 to 99 percent LV bypass was achieved during which MVO2 was reduced 28.1 percent from controls. LV decompression produced a maximum S-T reduction of 63 percent during which MVO2 was reduced 50.2 percent from controls. The final infarct size for Group II was 21.1 +/- 0.4 Gm. per 100 Gm of LV. This study indicates that larger degrees of bypass are required to decrease the ischemic areas encountered in larger infarctions. LV decompression is most beneficial in reducing ischemic myocardium surrounding large infarctions.", "contents": "Quantitative evaluation of left ventricular bypass in reducing myocardial ischemia. A controlled study was undertaken to evaluate the relationship between myocardial oxygen consumption (MVO2) and epicardial S-T segment improvement in an ischemic heart preparation during left ventricular (LV) bypass. Twelve mongrel dogs were prepared with left ventricular-aortic bypass, coronary sinus cannulation, and a multiple point epicardial electrocardiographic technique. The left anterior descending coronary artery was ligated low (Group I) or high (Group II) after baseline studies. After ischemic baseline studies, increasing degrees of LV bypass were performed at a constant mean aortic pressure and heart rate. The final infarct size was determined by the nitroblue tetrazolium staining technique. Group I--a significant reduction in the average (S-T) segment elevation occurred during 50 to 59 percent of LV bypass during which MVO2 was reduced 10.9 percent from controls. A maximum S-T reduction of 75 percent occurred during LV decompression during which MVO2 was reduced 52.8 percent from controls. The final infarct size for Group I was 9.8 +/- 0.9 Gm. per 100 Gm. of LV. Group II--a significant reduction in S-T segment elevation occurred only after 90 to 99 percent LV bypass was achieved during which MVO2 was reduced 28.1 percent from controls. LV decompression produced a maximum S-T reduction of 63 percent during which MVO2 was reduced 50.2 percent from controls. The final infarct size for Group II was 21.1 +/- 0.4 Gm. per 100 Gm of LV. This study indicates that larger degrees of bypass are required to decrease the ischemic areas encountered in larger infarctions. LV decompression is most beneficial in reducing ischemic myocardium surrounding large infarctions."}
{"id": "PMID:1083571", "title": "Aluminium localization in amphibian skin with the electron microprobe and ion microanalyzer.", "content": "EMP and IMA studies show the presence of A1 in the epidermis of larvae and young adults of Discoglossus pictus and Rana esculenta, which disappears during later stages of development. A1 is practically absent in the epidermis of Xenopus laevis and Pleurodeles waltlii. The localization of A1 with EMP and IMA supports the biochemical results. The function of A1 in the epidermis is not known at present.", "contents": "Aluminium localization in amphibian skin with the electron microprobe and ion microanalyzer. EMP and IMA studies show the presence of A1 in the epidermis of larvae and young adults of Discoglossus pictus and Rana esculenta, which disappears during later stages of development. A1 is practically absent in the epidermis of Xenopus laevis and Pleurodeles waltlii. The localization of A1 with EMP and IMA supports the biochemical results. The function of A1 in the epidermis is not known at present."}
{"id": "PMID:1083572", "title": "Diagnosis and management of onyalai.", "content": "Onyalai is diagnosed by finding the combination of haemorrhagic blisters in the mouth and severe thrombocytopenia, and by excluding other possible causes of thrombocytopenia. Patients improve rapidly with appropriate therapy. Haemorrhagic shock should be corrected with intravenous fluid and blood transfusion. Corticosteroids should be given in large doses initially, then tapered off and stopped over a period of several weeks. Occasional patients may require more prolonged steroid therapy.", "contents": "Diagnosis and management of onyalai. Onyalai is diagnosed by finding the combination of haemorrhagic blisters in the mouth and severe thrombocytopenia, and by excluding other possible causes of thrombocytopenia. Patients improve rapidly with appropriate therapy. Haemorrhagic shock should be corrected with intravenous fluid and blood transfusion. Corticosteroids should be given in large doses initially, then tapered off and stopped over a period of several weeks. Occasional patients may require more prolonged steroid therapy."}
{"id": "PMID:1083575", "title": "Selection of patients with urinary incontinence for application of functional electrical stimulation.", "content": "Contradictory data from the literature along with our own experience using functional electrical stimulation (FES) have facilitated the determination of objective criteria for the application of FES in correcting urinary incontinence. Simultaneous urodynamic, neurophysiological, and radiological examinations employed during our studies enabled us to determine changes in these parameters due to FEX. Among a large number of patients with urinary incontinence (for example: paraplegics, patients with spina bifida, stress incontinence following prostate operations, or resistant enuresis) we have determined that FES is appropriate if applied to properly selected patients. FES is indicated on the basis of the following criteria: the degree of morphological lesion of the urinary tract, the peripheral denervation of the muscles of the pelvic floor, the condition of the spinal center for miction, and, above all the quality of response to FES. FES not only activates the closing muscles of the bladder, but also inhibits the reflex contractions of the detrusor. Here, it should be noted that FES is indicated for cases of reflex incontinence. During FES of the pelvic floor muscles intravesical pressure normally diminishes and the reflex contractions of the detrusor subside. Sometimes, however, the reflex of miction occurs. In such a case, two reflex mechanisms are engaged. Their equilibrium, i.e. controlled miction, can be achieved with an afferent in flow which inhibits the reflex of miction. The use of FES for the correction of urinary incontinence can be a very good method if the FES parameters are individually chosen and patients properly selected.", "contents": "Selection of patients with urinary incontinence for application of functional electrical stimulation. Contradictory data from the literature along with our own experience using functional electrical stimulation (FES) have facilitated the determination of objective criteria for the application of FES in correcting urinary incontinence. Simultaneous urodynamic, neurophysiological, and radiological examinations employed during our studies enabled us to determine changes in these parameters due to FEX. Among a large number of patients with urinary incontinence (for example: paraplegics, patients with spina bifida, stress incontinence following prostate operations, or resistant enuresis) we have determined that FES is appropriate if applied to properly selected patients. FES is indicated on the basis of the following criteria: the degree of morphological lesion of the urinary tract, the peripheral denervation of the muscles of the pelvic floor, the condition of the spinal center for miction, and, above all the quality of response to FES. FES not only activates the closing muscles of the bladder, but also inhibits the reflex contractions of the detrusor. Here, it should be noted that FES is indicated for cases of reflex incontinence. During FES of the pelvic floor muscles intravesical pressure normally diminishes and the reflex contractions of the detrusor subside. Sometimes, however, the reflex of miction occurs. In such a case, two reflex mechanisms are engaged. Their equilibrium, i.e. controlled miction, can be achieved with an afferent in flow which inhibits the reflex of miction. The use of FES for the correction of urinary incontinence can be a very good method if the FES parameters are individually chosen and patients properly selected."}
{"id": "PMID:1083576", "title": "A critical look at incontinence radio-implants.", "content": "Indwelling electrical stimulation of the pelvic floor by a radio-implant benefits some cases of problem or recurrent urinary incontinence. It is not possible to predict success or failure with a given patient. The only worthwhile criterion for selection is the presence of contraction of pelvic floor musculature in response to voluntary effort or trial electrical stimulation. The results are not simply explained by postulating electrically induced closure of the urethra. There may be conscious enhancement of the urinary sphincter mechanism. Re-education of voluntary sphincter muscles by electrophysiotherapy may occur. Reflex inhibition of the detrusor may occur. The surgery involved in inserting an implant restores continence in some patients.", "contents": "A critical look at incontinence radio-implants. Indwelling electrical stimulation of the pelvic floor by a radio-implant benefits some cases of problem or recurrent urinary incontinence. It is not possible to predict success or failure with a given patient. The only worthwhile criterion for selection is the presence of contraction of pelvic floor musculature in response to voluntary effort or trial electrical stimulation. The results are not simply explained by postulating electrically induced closure of the urethra. There may be conscious enhancement of the urinary sphincter mechanism. Re-education of voluntary sphincter muscles by electrophysiotherapy may occur. Reflex inhibition of the detrusor may occur. The surgery involved in inserting an implant restores continence in some patients."}
{"id": "PMID:1083577", "title": "Maximum perineal stimulation for urinary incontinence.", "content": "Maximum stimulation of the pelvic floor has been advocated for the treatment of urinary incontinence associated with defective urethral closure mechanisms. Information in the literature was imprecise as to what constitutes a maximum stimulation. With one exception, published results are not encouraging when analysed. None of our 19 patients showed improvement. One had enhanced contraction when using an anal plug electrode following maximum stimulation, and this effect may warrant further study.", "contents": "Maximum perineal stimulation for urinary incontinence. Maximum stimulation of the pelvic floor has been advocated for the treatment of urinary incontinence associated with defective urethral closure mechanisms. Information in the literature was imprecise as to what constitutes a maximum stimulation. With one exception, published results are not encouraging when analysed. None of our 19 patients showed improvement. One had enhanced contraction when using an anal plug electrode following maximum stimulation, and this effect may warrant further study."}
{"id": "PMID:1083578", "title": "Electrical stimulation for incontinence. Technique, selection, and results.", "content": "Incontinence due to hyperreflexic bladder and/or pelvic floor weakness can be corrected by chronic functional electrical stimulation (FES). Cystometry, electromyography of pelvic floor muscles, and anal sphincter pressure measurements with and without electrical stimulation determines if chronic FES will be successful. Post-acute stimulation improvement occurred in patients with incontinence due to hyperreflexic bladder and/or pelvic floor weakness. A success rate of 92 per cent was achieved with chronic FES in incontinent patients with this method of selection.", "contents": "Electrical stimulation for incontinence. Technique, selection, and results. Incontinence due to hyperreflexic bladder and/or pelvic floor weakness can be corrected by chronic functional electrical stimulation (FES). Cystometry, electromyography of pelvic floor muscles, and anal sphincter pressure measurements with and without electrical stimulation determines if chronic FES will be successful. Post-acute stimulation improvement occurred in patients with incontinence due to hyperreflexic bladder and/or pelvic floor weakness. A success rate of 92 per cent was achieved with chronic FES in incontinent patients with this method of selection."}
{"id": "PMID:1083573", "title": "Lymphocyte functions in the rat spleen at various times after urethan administration.", "content": "The effect of urethan on various spleen-lymphocyte functions was studied in the rat for 45 days after administration of the carcinogen. For 3 days following the last injection of urethan, the total cell number and LPS reactivity were greatly reduced. Certain reactivities, probably T-cell dependent functions, such as responsiveness to the in vitro mitogenic effect of PHA, Con A, and primary stimulation by histocompatibility alloantigens in the one-way reaction, were selectively enriched during the first 2 days. Thereafter several dissociations of these lymphocyte functions can be observed: i.e., various intervals succeed during which one function may be enriched and other(s) diminished. It seems that the kinetics of enrichment, decay and recovery of the T-cell subsets involved in the reactions investigated follow a distinctive profile for each one of them. It is suggested, as a working hypothesis, that the unbalanced situations derived from the time-course discordance of the quantitative changes in the various lymphocyte functions may allow, or even enhance, tumor development.", "contents": "Lymphocyte functions in the rat spleen at various times after urethan administration. The effect of urethan on various spleen-lymphocyte functions was studied in the rat for 45 days after administration of the carcinogen. For 3 days following the last injection of urethan, the total cell number and LPS reactivity were greatly reduced. Certain reactivities, probably T-cell dependent functions, such as responsiveness to the in vitro mitogenic effect of PHA, Con A, and primary stimulation by histocompatibility alloantigens in the one-way reaction, were selectively enriched during the first 2 days. Thereafter several dissociations of these lymphocyte functions can be observed: i.e., various intervals succeed during which one function may be enriched and other(s) diminished. It seems that the kinetics of enrichment, decay and recovery of the T-cell subsets involved in the reactions investigated follow a distinctive profile for each one of them. It is suggested, as a working hypothesis, that the unbalanced situations derived from the time-course discordance of the quantitative changes in the various lymphocyte functions may allow, or even enhance, tumor development."}
{"id": "PMID:1083579", "title": "Suppressor T cells in the pathogenesis of hypogammaglobulinemia associated with a thymoma.", "content": "The nature of the immunological defect in patients with hypogammaglobulinemia associated with a thymoma was investigated using a technique established to study the differentiation of lymphocytes into immunoglobulin synthesizing and secreting cells. Exhaustively washed peripheral blood lymphocytes were cultured for 7 days in RPMI-1640 medium supplemented with fetal calf serum in the presence of the lectin, pokeweed mitogen. The IgG, IgA, and IgM synthesized and secreted into the medium were measured by competitive double antibody radio-immunoassays. Twenty-two normal individuals synthesized 1625 ng of IgG, 1270 ng of IgA, and 4910 ng of IgM per 2 million lymphocytes in culture. In contrast, the three patients with hypogammaglobulinemia and a thymoma synthesized less than 100 ng of each class of immunoglobulin. When lymphocytes from 2 of the 3 patients studied were cocultured with normal lymphocytes and pokeweed mitogen, the synthesis of immunoglobulin by normal lymphocytes was depressed by a factor of 66 to 97%. Co-cultue of purified T cells from the hypogammaglobulinemic patients with normal lymphocytes resulted in an 87% suppression of immunoglobulin synthesis by the normal cells. However, no suppression of immunoglobulin synthesis was observed when preparations of B cells and macrophages depleted of T cells from the hypogammaglobulinemic patients were co-cultured with normal lymphocytes. In addition, in control studies no such suppression of immunoglobulin synthesis was seen when normal cells were co-cultured with lymphocytes from unrelated normals, patients with isolated IgA deficiency, patients with chronic lymphocytic leukemia or patients with the Sezary syndrome, a T cell leukemia nor were they inhibited when incubated with T cells from unrelated normals. These observations suggest that in some patients the hypogammaglobulinemia associated with a thymoma may be caused or perpetuated by an abnormality of regulatory T cells which suppress the maturation of lymphocytes into antibody producing cells.", "contents": "Suppressor T cells in the pathogenesis of hypogammaglobulinemia associated with a thymoma. The nature of the immunological defect in patients with hypogammaglobulinemia associated with a thymoma was investigated using a technique established to study the differentiation of lymphocytes into immunoglobulin synthesizing and secreting cells. Exhaustively washed peripheral blood lymphocytes were cultured for 7 days in RPMI-1640 medium supplemented with fetal calf serum in the presence of the lectin, pokeweed mitogen. The IgG, IgA, and IgM synthesized and secreted into the medium were measured by competitive double antibody radio-immunoassays. Twenty-two normal individuals synthesized 1625 ng of IgG, 1270 ng of IgA, and 4910 ng of IgM per 2 million lymphocytes in culture. In contrast, the three patients with hypogammaglobulinemia and a thymoma synthesized less than 100 ng of each class of immunoglobulin. When lymphocytes from 2 of the 3 patients studied were cocultured with normal lymphocytes and pokeweed mitogen, the synthesis of immunoglobulin by normal lymphocytes was depressed by a factor of 66 to 97%. Co-cultue of purified T cells from the hypogammaglobulinemic patients with normal lymphocytes resulted in an 87% suppression of immunoglobulin synthesis by the normal cells. However, no suppression of immunoglobulin synthesis was observed when preparations of B cells and macrophages depleted of T cells from the hypogammaglobulinemic patients were co-cultured with normal lymphocytes. In addition, in control studies no such suppression of immunoglobulin synthesis was seen when normal cells were co-cultured with lymphocytes from unrelated normals, patients with isolated IgA deficiency, patients with chronic lymphocytic leukemia or patients with the Sezary syndrome, a T cell leukemia nor were they inhibited when incubated with T cells from unrelated normals. These observations suggest that in some patients the hypogammaglobulinemia associated with a thymoma may be caused or perpetuated by an abnormality of regulatory T cells which suppress the maturation of lymphocytes into antibody producing cells."}
{"id": "PMID:1083574", "title": "[Histological and immunological study of patients with lung and digestive tract tumors. Analysis of T and B lymphocytes from the peripheral blood and from the lymph nodes draining the tumor (author's transl)].", "content": "The nature of the immune response to the tumor cell is complex and has yet to be clearly defined. Although past research has foscused on the cytotoxic effect of T lymphocytes versus tumor cells, it has been shown in animal studies that B lymphocytes may also be implicated. Lymphocytes from patients with respiratory and digestive tract tumors were studied. B and T lymphocytes of peripheral blood and of draining lymph node tumors were studied using the following techniques: E rosettes (marker for T cells); membrane Ig, EAC rosettes, aggregated-Ig (marker for B lymphocytes); PHA and PKW in vitro response of lymphocytes using tritiated thymidine incorporation. It was observed that both groups of patients had normal or depressed B and T populations. PHA response was depressed in the majority of the cases with lung tumor. No difference was observed between lymphocytes from peripheral blood and from lymph node suspensions. As in normal lymph nodes the EAC rosettes were constantly observed in the cortical area of lymph node draining tumors. The immune defect observed in part of these cases is discussed in relation to the local and general immunological factors probably responsible for this defect.", "contents": "[Histological and immunological study of patients with lung and digestive tract tumors. Analysis of T and B lymphocytes from the peripheral blood and from the lymph nodes draining the tumor (author's transl)]. The nature of the immune response to the tumor cell is complex and has yet to be clearly defined. Although past research has foscused on the cytotoxic effect of T lymphocytes versus tumor cells, it has been shown in animal studies that B lymphocytes may also be implicated. Lymphocytes from patients with respiratory and digestive tract tumors were studied. B and T lymphocytes of peripheral blood and of draining lymph node tumors were studied using the following techniques: E rosettes (marker for T cells); membrane Ig, EAC rosettes, aggregated-Ig (marker for B lymphocytes); PHA and PKW in vitro response of lymphocytes using tritiated thymidine incorporation. It was observed that both groups of patients had normal or depressed B and T populations. PHA response was depressed in the majority of the cases with lung tumor. No difference was observed between lymphocytes from peripheral blood and from lymph node suspensions. As in normal lymph nodes the EAC rosettes were constantly observed in the cortical area of lymph node draining tumors. The immune defect observed in part of these cases is discussed in relation to the local and general immunological factors probably responsible for this defect."}
{"id": "PMID:1083581", "title": "[Esophageal-gastric hemorrhage in patients with portal hypertension treated by splenectomy].", "content": "The authors report their observations over 336 patients previously subjected to splenectomy for the portal hypertension syndrome. Prior to surgery 66.4% of patients showed esophageal varices, 43.7% - bleeding. After the operation venous varices were noted in 48.2% of patients, bleeding - in 34% of all patients. The causes of hemorrhage occurred in later terms after splenectomy are analysed. A conclusion is made concerning the way how to minimize a probability of hemorrhage recurrence following splenectomy.", "contents": "[Esophageal-gastric hemorrhage in patients with portal hypertension treated by splenectomy]. The authors report their observations over 336 patients previously subjected to splenectomy for the portal hypertension syndrome. Prior to surgery 66.4% of patients showed esophageal varices, 43.7% - bleeding. After the operation venous varices were noted in 48.2% of patients, bleeding - in 34% of all patients. The causes of hemorrhage occurred in later terms after splenectomy are analysed. A conclusion is made concerning the way how to minimize a probability of hemorrhage recurrence following splenectomy."}
{"id": "PMID:1083582", "title": "[Complications in the use of proteinase inhibitors].", "content": "It is well known that the use of proteolysis inhibitors of animal origin can result in complications of an allergic character up to anaphylactic shock. More frequently such complications according to the authors' data occurred in intraarticular or paraarticular injection of drugs in patients suffering non-specific infectious arthritis. Some useful recommendations are given.", "contents": "[Complications in the use of proteinase inhibitors]. It is well known that the use of proteolysis inhibitors of animal origin can result in complications of an allergic character up to anaphylactic shock. More frequently such complications according to the authors' data occurred in intraarticular or paraarticular injection of drugs in patients suffering non-specific infectious arthritis. Some useful recommendations are given."}
{"id": "PMID:1083589", "title": "[Indices of cellular and humoral immunity following surgery intervention for malignant bone tumors].", "content": "The immunity indices were studied in 35 patients, operated upon for various types of osteosarcomas, as well as the nonspecific reactivity of T-and B-lymphocytes and the specific immune response of lymphocytes. The system of peripheral T-lymphocytes in these patients is being inhibited with the primary tumor progression and an unfavourable postoperative course. In a continuous (from 1 to 15 years) favourable course of the disease postoperatively a persistent restoration of T-lymphocytes reactivity was noted. The specific antitumor immunity was found at every stage of the disease, but its suppression with serum (a blocking effect) seemed to be an unfavourable factor from the prognostic point of view, it was more frequently observed in patients with a progressive tumor growth. The operation proper after a provisional nonspecific suppression resulted in enhancement of the immune reactivity.", "contents": "[Indices of cellular and humoral immunity following surgery intervention for malignant bone tumors]. The immunity indices were studied in 35 patients, operated upon for various types of osteosarcomas, as well as the nonspecific reactivity of T-and B-lymphocytes and the specific immune response of lymphocytes. The system of peripheral T-lymphocytes in these patients is being inhibited with the primary tumor progression and an unfavourable postoperative course. In a continuous (from 1 to 15 years) favourable course of the disease postoperatively a persistent restoration of T-lymphocytes reactivity was noted. The specific antitumor immunity was found at every stage of the disease, but its suppression with serum (a blocking effect) seemed to be an unfavourable factor from the prognostic point of view, it was more frequently observed in patients with a progressive tumor growth. The operation proper after a provisional nonspecific suppression resulted in enhancement of the immune reactivity."}
{"id": "PMID:1083597", "title": "[Revascularization of the ischemic heart muscle in disseminated coronary sclerosis].", "content": "The author presents his own experience in bypass operations performed on 123 patients suffering from ischaemic heart disease. The paper covers the period from October 1969 to April 1974. The total mortality in this group of 123 patients was 7%. Post-operative myocardial infarction took place in 11% of the cases. The control examination made 6 months after operation showed the patency of bypass in 75% of patients. Proliferative changes were found in 10% of the cases with venous bypass. Arterialisation of the cardiac veins was performed on 21 patients because of disseminated occlusion disease of the coronary arteries. Early results are very encouraging. However, a longer observation period is needed.", "contents": "[Revascularization of the ischemic heart muscle in disseminated coronary sclerosis]. The author presents his own experience in bypass operations performed on 123 patients suffering from ischaemic heart disease. The paper covers the period from October 1969 to April 1974. The total mortality in this group of 123 patients was 7%. Post-operative myocardial infarction took place in 11% of the cases. The control examination made 6 months after operation showed the patency of bypass in 75% of patients. Proliferative changes were found in 10% of the cases with venous bypass. Arterialisation of the cardiac veins was performed on 21 patients because of disseminated occlusion disease of the coronary arteries. Early results are very encouraging. However, a longer observation period is needed."}
{"id": "PMID:1083598", "title": "[Permeation-increasing activity of puncture-fluids: 2. Chromatographic and immunoelectrophoretic studies].", "content": "A human pleural effusion was separated by gel chromatography, DEAE-chromatography, disk-electrophoresis and immunoelectrophoresis. The fractions obtained were tested for the permeation-increasing activity. IgA, IgM, IgG, transferrin and haptoglobin are not responsible for a permeation-increasing effect. Albumin, alpha1-antitrypsin, acid alpha1-glycoprotein and alpha2-macroglobulin appear only in permeation-active fractions. Of these proteins, however, no direct effect on the permeability of animal membranes is known. It is discussed, whether proteolytic enzymes, which may be bound to these proteins are responsible for the increase of permeation.", "contents": "[Permeation-increasing activity of puncture-fluids: 2. Chromatographic and immunoelectrophoretic studies]. A human pleural effusion was separated by gel chromatography, DEAE-chromatography, disk-electrophoresis and immunoelectrophoresis. The fractions obtained were tested for the permeation-increasing activity. IgA, IgM, IgG, transferrin and haptoglobin are not responsible for a permeation-increasing effect. Albumin, alpha1-antitrypsin, acid alpha1-glycoprotein and alpha2-macroglobulin appear only in permeation-active fractions. Of these proteins, however, no direct effect on the permeability of animal membranes is known. It is discussed, whether proteolytic enzymes, which may be bound to these proteins are responsible for the increase of permeation."}
{"id": "PMID:1083599", "title": "[Experiences and results in the surgical management of bleeding esophageal varices using fundectomy].", "content": "1966, 1967, and 1971 we published the good results with the \"Transsectionsligature\" of bleeding esophageal varices. The bleeding segment is nearly in all cases the angiomusculare strech closure segment in the terminal portion. In the last 27 patients we used the fundektomie of the stomach together with a splenektomie to stop the bleeding. Fundektomie abolished ulcusdisposition and splenektomie diminished the development of anaemia. Encephalopathy does not occur after these operations. All patients with a healthy liver remained alive. Cirrhotic patients come to death through their poor liver function. The selection of patients is the remaining problem.", "contents": "[Experiences and results in the surgical management of bleeding esophageal varices using fundectomy]. 1966, 1967, and 1971 we published the good results with the \"Transsectionsligature\" of bleeding esophageal varices. The bleeding segment is nearly in all cases the angiomusculare strech closure segment in the terminal portion. In the last 27 patients we used the fundektomie of the stomach together with a splenektomie to stop the bleeding. Fundektomie abolished ulcusdisposition and splenektomie diminished the development of anaemia. Encephalopathy does not occur after these operations. All patients with a healthy liver remained alive. Cirrhotic patients come to death through their poor liver function. The selection of patients is the remaining problem."}
{"id": "PMID:1083600", "title": "[Ectopic pregnancy near term (case-report) (author's transl)].", "content": "Description of a case of ectopic pregnancy during the last 3 months with a living, malformed and underdeveloped neonate. Possible treatment is discussed: where as in our case there is a secondary ectopic pregnancy (i.e.a pregnancy started primarily in the tube and still in vascular contact with it but secondarily ectopic) removal of placenta following ligation of the main vessels arising from the tube is possible and recommended. In the much rarer primary ectopic pregnancy with primary nidus in the abdomen, removal of the placenta is dangerous and not advised because of the danger of diffuse hemorrhage.", "contents": "[Ectopic pregnancy near term (case-report) (author's transl)]. Description of a case of ectopic pregnancy during the last 3 months with a living, malformed and underdeveloped neonate. Possible treatment is discussed: where as in our case there is a secondary ectopic pregnancy (i.e.a pregnancy started primarily in the tube and still in vascular contact with it but secondarily ectopic) removal of placenta following ligation of the main vessels arising from the tube is possible and recommended. In the much rarer primary ectopic pregnancy with primary nidus in the abdomen, removal of the placenta is dangerous and not advised because of the danger of diffuse hemorrhage."}
{"id": "PMID:1083611", "title": "Acute radiological investigation of oesophagus and stomach in the event of massive upper gastro-intestinal tract bleeding.", "content": "Acute radiological investigation of the oesophagus, stomach and duodenum is a routine in patients admitted to the first surgical department of Kommunehospitalet, Copenhagen, with upper gastro-intestinal tract hemorrhage. In the period 1969-1972, 294 patients were admitted with hematemesis and/or melena. The acute radiological study was undertaken in 190 of these patients (65%). Whenever possible, confirmation of the acute primary radiological diagnosis was sought at acute operation, subsequent elective operation, by repeated radiological study, by gastroscopy, or at post mortem. 19 acute investigations were inadequate, and in a further 20 patients no studies subsequent to the acute study were made. There remain 151 patients, and the primary diagnosis could be confirmed in 111 (74%). 45 patients underwent acute operation, and primary diagnosis was confirmed in 66%. The most frequent primary diagnosis was duodenal ulcer (78 of 151 patients), which was verified in 63 instances (81%). In 8% of these patients subsequent definitive diagnosis of gastric ulcer was made, and in 11% no cause of bleeding could be demonstrated. Primary diagnosis of gastric ulcer in 28 patients could be confirmed in 85%. Primary diagnosis of oesophageal varices was made in 9 patients and confirmed in 7 (78%). One patient had a carcinoma of the stomach, and this was also the acute radiological diagnosis. In one patient primary diagnosis of oesophageal ulcer was made, but this could not be confirmed. In 34 patients the acute study failed to point the cause of bleeding, and in 50% of these patients subsequent examination was also non-productive. 26% subsequently evidenced duodenal ulcer; 11% gastric ulcer; 6% carcinoma of the stomach; 3% oesophageal varices; and 3%--one patient--a bleeding vessel in the fundus of the stomach. The acute radiological study was complication free. The place of the study in acute diagnosis of the patient with upper gastro-intestinal tract bleeding is discussed.", "contents": "Acute radiological investigation of oesophagus and stomach in the event of massive upper gastro-intestinal tract bleeding. Acute radiological investigation of the oesophagus, stomach and duodenum is a routine in patients admitted to the first surgical department of Kommunehospitalet, Copenhagen, with upper gastro-intestinal tract hemorrhage. In the period 1969-1972, 294 patients were admitted with hematemesis and/or melena. The acute radiological study was undertaken in 190 of these patients (65%). Whenever possible, confirmation of the acute primary radiological diagnosis was sought at acute operation, subsequent elective operation, by repeated radiological study, by gastroscopy, or at post mortem. 19 acute investigations were inadequate, and in a further 20 patients no studies subsequent to the acute study were made. There remain 151 patients, and the primary diagnosis could be confirmed in 111 (74%). 45 patients underwent acute operation, and primary diagnosis was confirmed in 66%. The most frequent primary diagnosis was duodenal ulcer (78 of 151 patients), which was verified in 63 instances (81%). In 8% of these patients subsequent definitive diagnosis of gastric ulcer was made, and in 11% no cause of bleeding could be demonstrated. Primary diagnosis of gastric ulcer in 28 patients could be confirmed in 85%. Primary diagnosis of oesophageal varices was made in 9 patients and confirmed in 7 (78%). One patient had a carcinoma of the stomach, and this was also the acute radiological diagnosis. In one patient primary diagnosis of oesophageal ulcer was made, but this could not be confirmed. In 34 patients the acute study failed to point the cause of bleeding, and in 50% of these patients subsequent examination was also non-productive. 26% subsequently evidenced duodenal ulcer; 11% gastric ulcer; 6% carcinoma of the stomach; 3% oesophageal varices; and 3%--one patient--a bleeding vessel in the fundus of the stomach. The acute radiological study was complication free. The place of the study in acute diagnosis of the patient with upper gastro-intestinal tract bleeding is discussed."}
{"id": "PMID:1083608", "title": "[EA rosettes in patients with progressive chronic polyarthritis].", "content": "One subpopulation of human lymphocytes contains Fc-receptors that form rosettes with antibody (IgG) coated human erythrocytes (EA-rosettes). On the average 8.9% of the lymphocytes from patients with rheumatoid arthritis (RA) form EA-rosettes compared with 5.3% in controls. When the lymphocytes were washed three times the EA-rosettes increased to 21.2% for RA patients and 9.5% for controls. This procedure thus reveals more Fc-receptors on the lymphocytes from RA patients than on those from healthy controls. The number of T-lymphocytes was normal in RA patients and controls, no matter how many times the lymphocytes were washed.", "contents": "[EA rosettes in patients with progressive chronic polyarthritis]. One subpopulation of human lymphocytes contains Fc-receptors that form rosettes with antibody (IgG) coated human erythrocytes (EA-rosettes). On the average 8.9% of the lymphocytes from patients with rheumatoid arthritis (RA) form EA-rosettes compared with 5.3% in controls. When the lymphocytes were washed three times the EA-rosettes increased to 21.2% for RA patients and 9.5% for controls. This procedure thus reveals more Fc-receptors on the lymphocytes from RA patients than on those from healthy controls. The number of T-lymphocytes was normal in RA patients and controls, no matter how many times the lymphocytes were washed."}
{"id": "PMID:1083614", "title": "[\"Side effects\" in cortisone therapy].", "content": "Glucocorticosteroids are comprehensively acting agents and their therapeutically used derivatives - the cortisonoids - accordingly also have unwanted effects. In addition every pharmacodynamically active medication of these drugs in due time, induces adrenal atrophy and leads to the patient's dependence upon an adequate exogenous supply. This condition is then called \"cortisonism\". Such a development represents one of the most important risks of cortisone therapy in rheumatic disorders.", "contents": "[\"Side effects\" in cortisone therapy]. Glucocorticosteroids are comprehensively acting agents and their therapeutically used derivatives - the cortisonoids - accordingly also have unwanted effects. In addition every pharmacodynamically active medication of these drugs in due time, induces adrenal atrophy and leads to the patient's dependence upon an adequate exogenous supply. This condition is then called \"cortisonism\". Such a development represents one of the most important risks of cortisone therapy in rheumatic disorders."}
{"id": "PMID:1083615", "title": "[Autoimmune phenomena in D-penicillamine therapy].", "content": "In two patients with rheumatoid arthritis, immunoglobuline deposits in the skin were detected during D-penicillamine treatment. One patient presented with drug induced exanthema, the other with pemphigus erythematosus which was confirmed by clinical, histological, and immunofluorescent findings. During the development of the specific side-effect in one patient, an increase of ANA-titre and antibody-binding-capacity versus denaturated DNA was observed. Therefore in a prospective study of another ten patients during four months after onset of treatment with D-penicillamine, antinuclear antibodies and antibody-binding-capacity versus native and denaturated DNA were examined. The latter group of patients - without skin lesions - did not show any changes in the above mentioned parameters.", "contents": "[Autoimmune phenomena in D-penicillamine therapy]. In two patients with rheumatoid arthritis, immunoglobuline deposits in the skin were detected during D-penicillamine treatment. One patient presented with drug induced exanthema, the other with pemphigus erythematosus which was confirmed by clinical, histological, and immunofluorescent findings. During the development of the specific side-effect in one patient, an increase of ANA-titre and antibody-binding-capacity versus denaturated DNA was observed. Therefore in a prospective study of another ten patients during four months after onset of treatment with D-penicillamine, antinuclear antibodies and antibody-binding-capacity versus native and denaturated DNA were examined. The latter group of patients - without skin lesions - did not show any changes in the above mentioned parameters."}
{"id": "PMID:1083616", "title": "Nerve excitation generated by the action potential of muscle.", "content": "The effect of the electrical activity of muscle on the nerve in contact with it was investigated. The results obtained on isolated nerve-muscle preparations and on whole leg preparations of frog revealed that, under certain conditions, the action potential of the muscle is capable of producing excitation of the nerve adjacent to the muscle. These results suggest the hypothesis that the muscle activity could have an additional function in the excitatory processes by means of the electrical effect exerted on the nerve.", "contents": "Nerve excitation generated by the action potential of muscle. The effect of the electrical activity of muscle on the nerve in contact with it was investigated. The results obtained on isolated nerve-muscle preparations and on whole leg preparations of frog revealed that, under certain conditions, the action potential of the muscle is capable of producing excitation of the nerve adjacent to the muscle. These results suggest the hypothesis that the muscle activity could have an additional function in the excitatory processes by means of the electrical effect exerted on the nerve."}
{"id": "PMID:1083617", "title": "The significance of a crossed extensor hallucis response in neurologic disorders: a comparison with the Babinski sign.", "content": "The significance of a crossed extensor hallucis response on active flexion of the hip was analysed in various neurological disorders. It is concluded that this sign, not formerly described, is a pathological reflex or synkinesia. In the cooperative patient the crossed extensor response is a more sensitive indicator of a minor disturbance within the cortico-spinal motor pathways than the Babinski sign. With lesions above the foramen magnum a crossed extensor hallucis response is observed more frequently than the Babinski sign.", "contents": "The significance of a crossed extensor hallucis response in neurologic disorders: a comparison with the Babinski sign. The significance of a crossed extensor hallucis response on active flexion of the hip was analysed in various neurological disorders. It is concluded that this sign, not formerly described, is a pathological reflex or synkinesia. In the cooperative patient the crossed extensor response is a more sensitive indicator of a minor disturbance within the cortico-spinal motor pathways than the Babinski sign. With lesions above the foramen magnum a crossed extensor hallucis response is observed more frequently than the Babinski sign."}
{"id": "PMID:1083618", "title": "Plantar response during infancy.", "content": "Cross-sectional as well as longitudinal studies on full-term urban and rural infants demonstrated that the initial extensor plantar response present in the first half of the infancy changed to a flexor plantar response in the later half. The urban infants receiving nutritional supplements showed an advance of 4 weeks over the rural infants. This process of change-over of plantar response was delayed in pre-term infants. However, two pairs of twins showed plantar response almost identical to full-term urban infants.", "contents": "Plantar response during infancy. Cross-sectional as well as longitudinal studies on full-term urban and rural infants demonstrated that the initial extensor plantar response present in the first half of the infancy changed to a flexor plantar response in the later half. The urban infants receiving nutritional supplements showed an advance of 4 weeks over the rural infants. This process of change-over of plantar response was delayed in pre-term infants. However, two pairs of twins showed plantar response almost identical to full-term urban infants."}
{"id": "PMID:1083619", "title": "Central cloudy corneal dystrophy of Fran\u00e7ois.", "content": "A family is reported with central cloudy dystrophy of the cornea, as described by Fran\u00e7ois in 1956. The lesion consists of a grey zone deep in the stroma, made up of polygonal or rounded areas with indistinct margins, separated by apparently clear, normal stroma. No deposits are visible in the slit lamp. The anterior and posterior boundary layers appear normal. The corneal thickness is normal. Open angle glaucoma was present in the family. The pathogenesis of the dystrophy is unknown, and histopathological examination has not been described. The differential diagnosis is briefly discussed. The family tree is suggestive of a dominant mode of transmission.", "contents": "Central cloudy corneal dystrophy of Fran\u00e7ois. A family is reported with central cloudy dystrophy of the cornea, as described by Fran\u00e7ois in 1956. The lesion consists of a grey zone deep in the stroma, made up of polygonal or rounded areas with indistinct margins, separated by apparently clear, normal stroma. No deposits are visible in the slit lamp. The anterior and posterior boundary layers appear normal. The corneal thickness is normal. Open angle glaucoma was present in the family. The pathogenesis of the dystrophy is unknown, and histopathological examination has not been described. The differential diagnosis is briefly discussed. The family tree is suggestive of a dominant mode of transmission."}
{"id": "PMID:1083620", "title": "An unusual case of histiocytosis X in the spine.", "content": "A case of spontaneously healing histiocytosis X followed over 9 years is presented. The lesion was located in the cervical and dorsal spine and had unusual radiological features such as soft tissue swelling, involvement of the pedicles and recovery with fusion between adjacent vertebra.", "contents": "An unusual case of histiocytosis X in the spine. A case of spontaneously healing histiocytosis X followed over 9 years is presented. The lesion was located in the cervical and dorsal spine and had unusual radiological features such as soft tissue swelling, involvement of the pedicles and recovery with fusion between adjacent vertebra."}
{"id": "PMID:1083622", "title": "Angular velocity of the slow phase in experimental and clinical lesions of the vestibular apparatus.", "content": "The authors present the results of their experimental and clinical observations on the occurrence of regularity in the course of angular velocity of the slow phase of nystagmus. They have found that the regularity is rather distinctly pronounced in peripheral lesions of the vestibular apparatus. This observation may be complementary to the interpretation of electronystagmographic recordings.", "contents": "Angular velocity of the slow phase in experimental and clinical lesions of the vestibular apparatus. The authors present the results of their experimental and clinical observations on the occurrence of regularity in the course of angular velocity of the slow phase of nystagmus. They have found that the regularity is rather distinctly pronounced in peripheral lesions of the vestibular apparatus. This observation may be complementary to the interpretation of electronystagmographic recordings."}
{"id": "PMID:1083623", "title": "Surface structures influencing the migration in vivo ecotaxis of murine thymocytes, T- and B-cells.", "content": "The effect of protease and neuraminidase treatment on the ecotaxis of radioactively labelled thymocytes, T- and B-cells was studied by scintillation counting and autoradiography. Each enzyme had a similar effect on all cell types. Both protease and neuraminidase treatment strongly reduced the migration to the lymph nodes while the distribution to the spleen was more profoundly reduced by neuraminidase than by protease. In contrast, a similar and less pronounced effect of the enzymatic treatments on the circulation to the Peyers patches was found. The possible structure of the surface \"receptors\" involved is discussed in view of the known effects of these enzymes on the cell membranes.", "contents": "Surface structures influencing the migration in vivo ecotaxis of murine thymocytes, T- and B-cells. The effect of protease and neuraminidase treatment on the ecotaxis of radioactively labelled thymocytes, T- and B-cells was studied by scintillation counting and autoradiography. Each enzyme had a similar effect on all cell types. Both protease and neuraminidase treatment strongly reduced the migration to the lymph nodes while the distribution to the spleen was more profoundly reduced by neuraminidase than by protease. In contrast, a similar and less pronounced effect of the enzymatic treatments on the circulation to the Peyers patches was found. The possible structure of the surface \"receptors\" involved is discussed in view of the known effects of these enzymes on the cell membranes."}
{"id": "PMID:1083624", "title": "Transfer factor, sarcoidosis and cellular immunity.", "content": "A 27-year-old man with disseminated sarcoidosis was treated with repeated injections of Transfer Factor. Grossly abnormal immunological in vitro parameters were normalized, but in spite of these changes the clinical condition deteriorated.", "contents": "Transfer factor, sarcoidosis and cellular immunity. A 27-year-old man with disseminated sarcoidosis was treated with repeated injections of Transfer Factor. Grossly abnormal immunological in vitro parameters were normalized, but in spite of these changes the clinical condition deteriorated."}
{"id": "PMID:1083621", "title": "[A biochemical and cytological explanation of cochlear otospongiosis].", "content": "In a study of 250 otosclerotic perilymphatic fluids, it was found statistically that in the presence of developing inner ear deafness, 90% of cases will demonstrate increased trypsin activity. Conversely, elevated antitrypsin levels are found in the absence of developing inner ear deafness.", "contents": "[A biochemical and cytological explanation of cochlear otospongiosis]. In a study of 250 otosclerotic perilymphatic fluids, it was found statistically that in the presence of developing inner ear deafness, 90% of cases will demonstrate increased trypsin activity. Conversely, elevated antitrypsin levels are found in the absence of developing inner ear deafness."}
{"id": "PMID:1083625", "title": "Posterior fossa meningiomas. A report of 30 cases.", "content": "The clinical symptoms of thirty patients with surgically treated posterior fossa meningiomas were reviewed. These included 16 cerebellopontine (CPA), 7 cerebellar, 4 carrefour-falco-tentorial (CFT), 2 foramen magnum, and 1 clivus meningiomas. Careful attention to clinical findings is necessary in diagnosing these tumors. With rare exception they have an insidious onset and even after having reached considerable size, the ensuing neurological abnormalities might not be revealing. On occasion there are false localizing neurological findings. Neuroradiological localization is essential to obtain accurate diagnosis and to plan the best operative approach. Radioactive brain scanning offers the best screening method. Although air studies remain useful, angiography of vessels of the posterior foss is assuming a dominant role in diagnosis and surgical planning.", "contents": "Posterior fossa meningiomas. A report of 30 cases. The clinical symptoms of thirty patients with surgically treated posterior fossa meningiomas were reviewed. These included 16 cerebellopontine (CPA), 7 cerebellar, 4 carrefour-falco-tentorial (CFT), 2 foramen magnum, and 1 clivus meningiomas. Careful attention to clinical findings is necessary in diagnosing these tumors. With rare exception they have an insidious onset and even after having reached considerable size, the ensuing neurological abnormalities might not be revealing. On occasion there are false localizing neurological findings. Neuroradiological localization is essential to obtain accurate diagnosis and to plan the best operative approach. Radioactive brain scanning offers the best screening method. Although air studies remain useful, angiography of vessels of the posterior foss is assuming a dominant role in diagnosis and surgical planning."}
{"id": "PMID:1083627", "title": "Nonspecific esterase activity in T cells.", "content": "Acid alpha-naphthyl acetate esterase activity characterizes the majority of peripheral T cells in mice. In addition to the simplicity of the cyto- or histochemical demonstration of esterase activity and the fact that this method may be applied to tissue sections and smears, the present study indicates that combination with lymphocyte surface markers may offer an important approach to the examination of differentiation pathways of peripheral T cells.", "contents": "Nonspecific esterase activity in T cells. Acid alpha-naphthyl acetate esterase activity characterizes the majority of peripheral T cells in mice. In addition to the simplicity of the cyto- or histochemical demonstration of esterase activity and the fact that this method may be applied to tissue sections and smears, the present study indicates that combination with lymphocyte surface markers may offer an important approach to the examination of differentiation pathways of peripheral T cells."}
{"id": "PMID:1083637", "title": "In vitro proliferative reactions by lymphocytes from both responder and \"low\" responder mice to (T,G)-A--L.", "content": "In summary (T,G)-A--L can induce specific in vitro lymphoproliferative reactions in LNC from immunized mice. From 3-8 weeks after immunization lymphocytes from responder mice react to a much greater degree than from low responders. These proliferative reactions are not specifically enhanced by supernatants of \"educated\" T cells. However, 2 to 3 months after immunization the lymphoproliferative response of the low responders rises to the same level as that of responder mice.", "contents": "In vitro proliferative reactions by lymphocytes from both responder and \"low\" responder mice to (T,G)-A--L. In summary (T,G)-A--L can induce specific in vitro lymphoproliferative reactions in LNC from immunized mice. From 3-8 weeks after immunization lymphocytes from responder mice react to a much greater degree than from low responders. These proliferative reactions are not specifically enhanced by supernatants of \"educated\" T cells. However, 2 to 3 months after immunization the lymphoproliferative response of the low responders rises to the same level as that of responder mice."}
{"id": "PMID:1083642", "title": "Bursa of Fabricus: uptake of radioactive particles and radiotoxic \"sealing\" of bursal follicles.", "content": "Making use of the fact that the avian bursa is uniquely equiped for uptake, degradation and storage of particulate matter from the cloacal lumen, we administered 239Pu(IV) perorally or via the cloaca to cause radiotoxic damage to the bursal tissue.  This resulted in a involution of the follicles, reduction of the lymphocyte mass, predominance of smaller lymphoid cells in the medulla as compared to controls, disappearance of tuft epithelia, formation of a sub-epithelial fibrous layer in many follicles and impairment or an almost complete loss of the capacity of the bursa to take up particulate matter. Concomitant with these changes was a reduction in the concentration of serum IgG. These findings are discussed in relation to the immunologic contact role of the bursa.", "contents": "Bursa of Fabricus: uptake of radioactive particles and radiotoxic \"sealing\" of bursal follicles. Making use of the fact that the avian bursa is uniquely equiped for uptake, degradation and storage of particulate matter from the cloacal lumen, we administered 239Pu(IV) perorally or via the cloaca to cause radiotoxic damage to the bursal tissue.  This resulted in a involution of the follicles, reduction of the lymphocyte mass, predominance of smaller lymphoid cells in the medulla as compared to controls, disappearance of tuft epithelia, formation of a sub-epithelial fibrous layer in many follicles and impairment or an almost complete loss of the capacity of the bursa to take up particulate matter. Concomitant with these changes was a reduction in the concentration of serum IgG. These findings are discussed in relation to the immunologic contact role of the bursa."}
{"id": "PMID:1083647", "title": "The need for transcription and translation for differentiation of bone marrow cells by thymic factors in man.", "content": "1. Two small populations of human marrow cells are differentiated to cells bearing T-cell markers by Thymosin F3 or F5. 2. Analysis with metabolic inhibitors indicates that transcription and translation of RNA are needed for induction of T cell markers (layer III). 3. a) At 4 hours incubation RNA synthesis is enhanced in layers III, IV and V and inhibited in layers I + II. b) At 20 hours incubation RNA synthesis is markedly enhanced in all cell layers. 4. a) At 5 hours incubation protein synthesis is suppressed in layers I + II and III, but not in IV and V. b) At 20 hours incubation protein synthesis is markedly increased only in layer III.", "contents": "The need for transcription and translation for differentiation of bone marrow cells by thymic factors in man. 1. Two small populations of human marrow cells are differentiated to cells bearing T-cell markers by Thymosin F3 or F5. 2. Analysis with metabolic inhibitors indicates that transcription and translation of RNA are needed for induction of T cell markers (layer III). 3. a) At 4 hours incubation RNA synthesis is enhanced in layers III, IV and V and inhibited in layers I + II. b) At 20 hours incubation RNA synthesis is markedly enhanced in all cell layers. 4. a) At 5 hours incubation protein synthesis is suppressed in layers I + II and III, but not in IV and V. b) At 20 hours incubation protein synthesis is markedly increased only in layer III."}
{"id": "PMID:1083654", "title": "Studies on suppressor T cells in tolerance.", "content": "(1) Evidence is provided that tolerance induced in mice to HSA depends on a suppressive mechanism which inhibits the responsiveness of potentially competent cells. (2) the suppression is apparently the function of suppressor T cells which are comparatively radiosensitive and are resistant to cortisone. (3) The cortisone resistant suppressor cells are apparently the progeny of cortisone-sensitive precursors, the differentiation of which into mature suppressors seems to be signalled by antigen. (4) The precursor lymphocytes are comparatively short functioning cells (about 14 days), while the mature suppressor T cells function for at least two months.", "contents": "Studies on suppressor T cells in tolerance. (1) Evidence is provided that tolerance induced in mice to HSA depends on a suppressive mechanism which inhibits the responsiveness of potentially competent cells. (2) the suppression is apparently the function of suppressor T cells which are comparatively radiosensitive and are resistant to cortisone. (3) The cortisone resistant suppressor cells are apparently the progeny of cortisone-sensitive precursors, the differentiation of which into mature suppressors seems to be signalled by antigen. (4) The precursor lymphocytes are comparatively short functioning cells (about 14 days), while the mature suppressor T cells function for at least two months."}
{"id": "PMID:1083656", "title": "Depletion of suppressor T cells in syngeneic chimeric mice.", "content": "The present results suggest that some T-cell activities of syngeneic chimeric mice such as T-cells involved in the antibody response to SRBC and MLC reaction are intact. On the other hand, suppressor T-cells involved in the regulation of the immune response to PVP and enhancement of 3LL tumor growth, and cells mediating CML reaction are damaged.", "contents": "Depletion of suppressor T cells in syngeneic chimeric mice. The present results suggest that some T-cell activities of syngeneic chimeric mice such as T-cells involved in the antibody response to SRBC and MLC reaction are intact. On the other hand, suppressor T-cells involved in the regulation of the immune response to PVP and enhancement of 3LL tumor growth, and cells mediating CML reaction are damaged."}
{"id": "PMID:1083659", "title": "Interference with antibody-feedback by irradiation, thymus cells, the allogeneic effect, and serum factors.", "content": "Low doses of irradiation, increased number of T cells, increased T cell activity as in the allogeneic effect, or early activation of an in vitro cell-mediated immune response all confer resistance to antibody feedback. In some of these systems, the resistance is directed against immunosuppression by intact IgG antibody whereas suppression by F(ab')2 antibody is either not changed or increased. These forms of interference with immunosuppression by antibody, are explained in terms of an Fc-dependent model for antibody-feedback regulation.", "contents": "Interference with antibody-feedback by irradiation, thymus cells, the allogeneic effect, and serum factors. Low doses of irradiation, increased number of T cells, increased T cell activity as in the allogeneic effect, or early activation of an in vitro cell-mediated immune response all confer resistance to antibody feedback. In some of these systems, the resistance is directed against immunosuppression by intact IgG antibody whereas suppression by F(ab')2 antibody is either not changed or increased. These forms of interference with immunosuppression by antibody, are explained in terms of an Fc-dependent model for antibody-feedback regulation."}
{"id": "PMID:1083661", "title": "Augmentation of cell mediated lysis (CML) by THF.", "content": "The in-vitro model of cell mediated lysis (CML) was used to study whether a thymic hormone (THF) participates in the processes which lead to generation of effector cells. It was found that THF increases the capacity of effector cells from spleens of intact mice in the CML assay. This effect of THF on generation of effector cells was manifested when THF was present during the mixed lymphocyte culture. On the other hand, addition of THF to the effector (CML) phase did not elevate the lytic capacity of killer cells. Moreover, THF compensated the impaired lytic capacity of effector cells from adult thymectomized mice and raised it to the level of intact mice. The results are compatible with the hypothesis that THF acts on the generation of CML effector cells of both intact and adult thymectomized mice.", "contents": "Augmentation of cell mediated lysis (CML) by THF. The in-vitro model of cell mediated lysis (CML) was used to study whether a thymic hormone (THF) participates in the processes which lead to generation of effector cells. It was found that THF increases the capacity of effector cells from spleens of intact mice in the CML assay. This effect of THF on generation of effector cells was manifested when THF was present during the mixed lymphocyte culture. On the other hand, addition of THF to the effector (CML) phase did not elevate the lytic capacity of killer cells. Moreover, THF compensated the impaired lytic capacity of effector cells from adult thymectomized mice and raised it to the level of intact mice. The results are compatible with the hypothesis that THF acts on the generation of CML effector cells of both intact and adult thymectomized mice."}
{"id": "PMID:1083663", "title": "Lymphocyte surface-attached immunoglobulins in some clinical conditions.", "content": "The TEAG rosette test was not devised as an immediate diagnostic indicator, but in order to detect gross differences over a period of time between the lymphocytes of patients with conditions where immune complexes may be formed, and those of normal people. In summary these results indicate that:- 1. Percentage TEAG rosettes were highly significantly increased in patients with SLE, active chronic hepatitis and carcinoma of lung compared with normal controls, when the tests were performed on suspensions, containing over 90% lymphocytes, separated from peripheral blood. 2. Estimates of mean B lymphocytes plus blood monocytes in the separated suspensions, as measured by EAC rosettes (and peroxidase and differential counts for monocytes) are exceeded by TEAG-rosetting cells in the patients tested. 3. Tests on patients with chronic autoimmune conditions (e.g. ACH and SLE) do not show a highly significant difference from normal controls with respect to mean total cells forming E-rosettes. 4. It may be speculated that some TEAG rosettes are formed by T-cells which could have immune complexes or autologous anti-lymphocyte globulin on their surface and that such a condition may account for the depressed T-cell function found in these conditions.", "contents": "Lymphocyte surface-attached immunoglobulins in some clinical conditions. The TEAG rosette test was not devised as an immediate diagnostic indicator, but in order to detect gross differences over a period of time between the lymphocytes of patients with conditions where immune complexes may be formed, and those of normal people. In summary these results indicate that:- 1. Percentage TEAG rosettes were highly significantly increased in patients with SLE, active chronic hepatitis and carcinoma of lung compared with normal controls, when the tests were performed on suspensions, containing over 90% lymphocytes, separated from peripheral blood. 2. Estimates of mean B lymphocytes plus blood monocytes in the separated suspensions, as measured by EAC rosettes (and peroxidase and differential counts for monocytes) are exceeded by TEAG-rosetting cells in the patients tested. 3. Tests on patients with chronic autoimmune conditions (e.g. ACH and SLE) do not show a highly significant difference from normal controls with respect to mean total cells forming E-rosettes. 4. It may be speculated that some TEAG rosettes are formed by T-cells which could have immune complexes or autologous anti-lymphocyte globulin on their surface and that such a condition may account for the depressed T-cell function found in these conditions."}
{"id": "PMID:1083665", "title": "Involvement of cells with 'B' properties in development of T helpers.", "content": "Cells bearing Ig on the membrane, fractionated from either 17 day embryonic livers or from normal adult bone marrow, when transferred to splenectomized-irradiated mice, lead to development of helper cells in the thymus of the recipients. The helper function was expressed when the recipients were stimulated with flagellin-MON and the thymus cells were cultured together with anti theta treated spleen cells and stimulated with DNP-MON. The response to DNP was not enhanced when the irradiated mice were inoculated with non Ig bearing cells. Helper activity was related to cells which were eliminated with anti theta antibodies and did not have detectable Ig. Hence, cells with 'B' properties may be involved in development of T helpers.", "contents": "Involvement of cells with 'B' properties in development of T helpers. Cells bearing Ig on the membrane, fractionated from either 17 day embryonic livers or from normal adult bone marrow, when transferred to splenectomized-irradiated mice, lead to development of helper cells in the thymus of the recipients. The helper function was expressed when the recipients were stimulated with flagellin-MON and the thymus cells were cultured together with anti theta treated spleen cells and stimulated with DNP-MON. The response to DNP was not enhanced when the irradiated mice were inoculated with non Ig bearing cells. Helper activity was related to cells which were eliminated with anti theta antibodies and did not have detectable Ig. Hence, cells with 'B' properties may be involved in development of T helpers."}
{"id": "PMID:1083667", "title": "Myocardial imaging in the noninvasive evaluation of patients with suspected ischemic heart disease.", "content": "Three noninvasive radioactive tracer techniques for evaluating patients with ischemic heart disease are described: (1) myocaridal perfusion imaging, (2) acute infarct imaging, and (3) the gated blood pool scan. Myocardial perfusion imaging with tracers that distribute in the myocardium in relation to regional blood flow allows detection of patients with transmural and nontransmural infarction by the finding of decreased tracer concentration in the affected region of the myocardium. If these tracers are injected at the time of maximal stress to patients with significant coronary arterial stenosis but without infarction, areas of transient ischemia can be identified as zones of decreased tracer concentration not found when an examination is performed at rest. Acute infarct imaging with tracers that localize in acutely damaged tissue permits separation of patients with acute myocardial necrosis from those without infarction and those with more chronic damage. The gated blood pool scan permits assessment of left ventricular function and regional wall motion. The measurement of ventricular volumes, ejection fraction and regional wall motion adds significantly to the determination of hemodynamic variables in assessing patients with acute infarction. The technique also permits detection of right ventricular dysfunction. Performance of a combination of these radioactive tracer techniques is often advantageous, particularly in patients with suspected infarction. The techniques can establish whether infarction is present, whether it is acute, where the damage is located and how extensive it is; they can also provide a measure of the effect of this damage on left ventricular function.", "contents": "Myocardial imaging in the noninvasive evaluation of patients with suspected ischemic heart disease. Three noninvasive radioactive tracer techniques for evaluating patients with ischemic heart disease are described: (1) myocaridal perfusion imaging, (2) acute infarct imaging, and (3) the gated blood pool scan. Myocardial perfusion imaging with tracers that distribute in the myocardium in relation to regional blood flow allows detection of patients with transmural and nontransmural infarction by the finding of decreased tracer concentration in the affected region of the myocardium. If these tracers are injected at the time of maximal stress to patients with significant coronary arterial stenosis but without infarction, areas of transient ischemia can be identified as zones of decreased tracer concentration not found when an examination is performed at rest. Acute infarct imaging with tracers that localize in acutely damaged tissue permits separation of patients with acute myocardial necrosis from those without infarction and those with more chronic damage. The gated blood pool scan permits assessment of left ventricular function and regional wall motion. The measurement of ventricular volumes, ejection fraction and regional wall motion adds significantly to the determination of hemodynamic variables in assessing patients with acute infarction. The technique also permits detection of right ventricular dysfunction. Performance of a combination of these radioactive tracer techniques is often advantageous, particularly in patients with suspected infarction. The techniques can establish whether infarction is present, whether it is acute, where the damage is located and how extensive it is; they can also provide a measure of the effect of this damage on left ventricular function."}
{"id": "PMID:1083668", "title": "Effects of coronary artery bypass grafting on resting and exercise hemodynamics in patients with stable angina pectoris: a prospective, randomized study.", "content": "In this prospective randomized study, resting and exercise hemodynamics were determined in the nonmedicated state before (\"entry\") and 1 year after coronary bypass surgery in 38 patients, and at entry and 1 year in 40 patients treated medically. The surgical group showed a significant decrease in mean pulmonary arterial wedge pressure during exercise (entry 23.5 +/- 6.1 [standard error of the mean] mm Hg, 1 year 18.9 +/- 1.0, P less than 0.02); an increase in cardiac index during exercise (entry 4.3 +/- 0.1 liter/min per m2, 1 year 4.6 +/- 0.1, P less than 0.05); an increase in resting mean arterial pressure (entry 94.5 +/- 2.2 mm Hg, 1 year 100.2 +/- 2.2, P less than 0.02); and an increase in resting heart rate (entry 68.5 +/- 1.9 beats/min, 1 year: 76.0 +/- 2.0, P less than 0.01). Maximal treadmill exercise performance also improved significantly in the surgical group of patients (entry 285 +/- 24 seconds, 1 year 382 +/- 24, P less than 0.002). There were no significant changes in these variables in the medically treated \"control\" group. The improvement in pulmonary arterial wedge pressure during exercise and in maximal treadmill exercise time in the surgical group as a whole was due to striking improvement in these variables in a subgroup of 16 surgical patients who had a more than 10 mm Hg increase in pulmonary arterial wedge pressure during exercise in their entry study. In this subgoup, considered to contain those patients with marked \"ischemicdysfunction,\" pulmonary arterial wedge pressure during exercise fell from 31.4 +/- 1.5 mm Hg (entry) to 19.l +/- 1.8 (1 year) (P less than 0.0001) and treadmill time increased from 217 +/- 24 seconds (entry) to 357 +/- 37 (1 year) (P less than 0.001). Thus, hemodynamic evidence of ischemic left ventricular dysfunction during stress may identify those patients who will show objective improvement in ventricular performance after bypass graft surgery.", "contents": "Effects of coronary artery bypass grafting on resting and exercise hemodynamics in patients with stable angina pectoris: a prospective, randomized study. In this prospective randomized study, resting and exercise hemodynamics were determined in the nonmedicated state before (\"entry\") and 1 year after coronary bypass surgery in 38 patients, and at entry and 1 year in 40 patients treated medically. The surgical group showed a significant decrease in mean pulmonary arterial wedge pressure during exercise (entry 23.5 +/- 6.1 [standard error of the mean] mm Hg, 1 year 18.9 +/- 1.0, P less than 0.02); an increase in cardiac index during exercise (entry 4.3 +/- 0.1 liter/min per m2, 1 year 4.6 +/- 0.1, P less than 0.05); an increase in resting mean arterial pressure (entry 94.5 +/- 2.2 mm Hg, 1 year 100.2 +/- 2.2, P less than 0.02); and an increase in resting heart rate (entry 68.5 +/- 1.9 beats/min, 1 year: 76.0 +/- 2.0, P less than 0.01). Maximal treadmill exercise performance also improved significantly in the surgical group of patients (entry 285 +/- 24 seconds, 1 year 382 +/- 24, P less than 0.002). There were no significant changes in these variables in the medically treated \"control\" group. The improvement in pulmonary arterial wedge pressure during exercise and in maximal treadmill exercise time in the surgical group as a whole was due to striking improvement in these variables in a subgroup of 16 surgical patients who had a more than 10 mm Hg increase in pulmonary arterial wedge pressure during exercise in their entry study. In this subgoup, considered to contain those patients with marked \"ischemicdysfunction,\" pulmonary arterial wedge pressure during exercise fell from 31.4 +/- 1.5 mm Hg (entry) to 19.l +/- 1.8 (1 year) (P less than 0.0001) and treadmill time increased from 217 +/- 24 seconds (entry) to 357 +/- 37 (1 year) (P less than 0.001). Thus, hemodynamic evidence of ischemic left ventricular dysfunction during stress may identify those patients who will show objective improvement in ventricular performance after bypass graft surgery."}
{"id": "PMID:1083669", "title": "Coronary artery bypass surgery for left main coronary artery disease.", "content": "The course of 146 consecutive patients with significant occlusive disease of the left main coronary artery who underwent coronary artery bypass surgery during a 4 year period is reviewed. Preoperatively, 11 patients were in New York Heart Association functional class II, 57 in class III and 78 in class IV. Seventy patients had progressive angina and 12 unstable angina. There were two operative deaths (surgical mortality rate 1.4 percent). Seven patients (4.8 percent) had a perioperative acute myocardial infarction. Complete follow-up has been achieved in the surgical survivors over an average period of 18.1 months; 77 percent of the surviving patients are completely asymptomatic and 19 percent are in functional class II. Four patients (2.8 percent) had a nonfatal late postoperative myocardial infarction and five (3.5 percent) died during the late postoperative period (3.3 percent annual mortality rate during a 2 to 47 month follow-up period). Postoperative cardiac catheterization studies performed in 35 patients an average of 12.1 months postoperatively revealed 78 percent of 80 grafts and patency of at least 1 graft in 93 percent of patients. Results of 42 (89 percent) of 47 near maximal treadmill stress tests were abnormal preoperatively compared with results of 14 (26 percent) of 54 postoperatively; in 74 percent of patients having both a preoperative and postoperative stress test, abnormal preoperative test results converted to normal after surgery. This study suggests that direct myocardial revascularization may offer an effective means of improving both the quality and duration of life in a patient with significant occlusive disease of the left main coronary artery.", "contents": "Coronary artery bypass surgery for left main coronary artery disease. The course of 146 consecutive patients with significant occlusive disease of the left main coronary artery who underwent coronary artery bypass surgery during a 4 year period is reviewed. Preoperatively, 11 patients were in New York Heart Association functional class II, 57 in class III and 78 in class IV. Seventy patients had progressive angina and 12 unstable angina. There were two operative deaths (surgical mortality rate 1.4 percent). Seven patients (4.8 percent) had a perioperative acute myocardial infarction. Complete follow-up has been achieved in the surgical survivors over an average period of 18.1 months; 77 percent of the surviving patients are completely asymptomatic and 19 percent are in functional class II. Four patients (2.8 percent) had a nonfatal late postoperative myocardial infarction and five (3.5 percent) died during the late postoperative period (3.3 percent annual mortality rate during a 2 to 47 month follow-up period). Postoperative cardiac catheterization studies performed in 35 patients an average of 12.1 months postoperatively revealed 78 percent of 80 grafts and patency of at least 1 graft in 93 percent of patients. Results of 42 (89 percent) of 47 near maximal treadmill stress tests were abnormal preoperatively compared with results of 14 (26 percent) of 54 postoperatively; in 74 percent of patients having both a preoperative and postoperative stress test, abnormal preoperative test results converted to normal after surgery. This study suggests that direct myocardial revascularization may offer an effective means of improving both the quality and duration of life in a patient with significant occlusive disease of the left main coronary artery."}
{"id": "PMID:1083670", "title": "Architecture of the marrow vasculature in three amphibian species and its significance in hematopoietic development.", "content": "Architecture of the bone marrow vasculature, particularly that of the femur, was analyzed in three amphibian species in relation to the early phylogeny of marrow hematopoiesis. A dye-injection method and histological techniques, including both serial sectioning and reconstruction methods, were used for this purpose. From these observations the following conclusions may be drawn. (1) Marrow hematopoiesis is absent from the femur of the urodelan (Triturus pyrrhogaster) and appears first in the femur of the primitive anuran (Xenopus laevis) (2) The site of primitive hematopoiesis (granulopoiesis) is the subendosteal region where the venous vascular net develops. (3) The primitive vascular architecture observed in the femur of Xenopus is characterized by the absence of a central vein. Subendosteal veins drain the blood from the bone marrow. A vein collateral to the primary artery appears in the femur of Rana catesbeiana, an advanced anuran, in which further development of both the subendosteal venous plexus and hematopoietic activity are noted. In both anura examined, the primitive blood sinuses form near the mid-shaft of the femur. The proliferation of mesenchymal elements containing dark pigment, presumably melanin, was also noted in this area. (4) The architecture of marrow vessels in Rana approaches the structure noted in mammalian bone marrow. (5) Fat tissue is observed in the urodelan bone marrow prior to the appearance of hematopoietic activity. This indicates that the formation of marrow fat is phylogenetically unrelated to the development of hematopoiesis. The present investigation on primitive hematopoiesis suggests that the development of hematopoietic activity is intimately related to the development of the marrow vasculature, particularly that of the subendosteal venous plexus. A favorable vascular arrangement may be necessary to allow active hematopoiesis.", "contents": "Architecture of the marrow vasculature in three amphibian species and its significance in hematopoietic development. Architecture of the bone marrow vasculature, particularly that of the femur, was analyzed in three amphibian species in relation to the early phylogeny of marrow hematopoiesis. A dye-injection method and histological techniques, including both serial sectioning and reconstruction methods, were used for this purpose. From these observations the following conclusions may be drawn. (1) Marrow hematopoiesis is absent from the femur of the urodelan (Triturus pyrrhogaster) and appears first in the femur of the primitive anuran (Xenopus laevis) (2) The site of primitive hematopoiesis (granulopoiesis) is the subendosteal region where the venous vascular net develops. (3) The primitive vascular architecture observed in the femur of Xenopus is characterized by the absence of a central vein. Subendosteal veins drain the blood from the bone marrow. A vein collateral to the primary artery appears in the femur of Rana catesbeiana, an advanced anuran, in which further development of both the subendosteal venous plexus and hematopoietic activity are noted. In both anura examined, the primitive blood sinuses form near the mid-shaft of the femur. The proliferation of mesenchymal elements containing dark pigment, presumably melanin, was also noted in this area. (4) The architecture of marrow vessels in Rana approaches the structure noted in mammalian bone marrow. (5) Fat tissue is observed in the urodelan bone marrow prior to the appearance of hematopoietic activity. This indicates that the formation of marrow fat is phylogenetically unrelated to the development of hematopoiesis. The present investigation on primitive hematopoiesis suggests that the development of hematopoietic activity is intimately related to the development of the marrow vasculature, particularly that of the subendosteal venous plexus. A favorable vascular arrangement may be necessary to allow active hematopoiesis."}
{"id": "PMID:1083672", "title": "A comparative study of intra-amniotic saline and two prostaglandin F2alpha dose schedules for midtrimester abortion.", "content": "The efficacy, side effects, and complications of two intra-amniotic PGF2alpha dose schedules and the unaugmented intra-amniotic instillation of saline are compared. All three methods resulted in satisfactory rates of abortion within a relatively short period of time and within clinically acceptable rates of complications. Each method has its advantages and disadvantages. Further large comparative studies were needed.", "contents": "A comparative study of intra-amniotic saline and two prostaglandin F2alpha dose schedules for midtrimester abortion. The efficacy, side effects, and complications of two intra-amniotic PGF2alpha dose schedules and the unaugmented intra-amniotic instillation of saline are compared. All three methods resulted in satisfactory rates of abortion within a relatively short period of time and within clinically acceptable rates of complications. Each method has its advantages and disadvantages. Further large comparative studies were needed."}
{"id": "PMID:1083673", "title": "Retinitis pigmentosa without pigment.", "content": "Sixty-eight consecutive patients with retinitis pigmentosa were studied to determine the frequency of the nonpigmented form of the disease. There was an overall incidence of 22%. Fifty percent of all cases had no characteristic pigmentation, if the duration of night vision difficulty was three years or less. The nonpigmented form of retinitis pigmentosa was frequently an early stage of the disease and not an unusual or atypical variant. Patients without the pigmentary changes characteristic of the disorder also showed less functional impairment: the electroretinographic b wave was more apt to be recordable, although impaired, and the rod threshold, as determined by dark adaptation measurement, was less elevated. The clinician should suspect retinitis pigmentosa, even in the absence of pigmentary changes, if there is a family history of the disorder, night blindness, peripheral field loss, and an impaired or nonrecordable electroretinographic response.", "contents": "Retinitis pigmentosa without pigment. Sixty-eight consecutive patients with retinitis pigmentosa were studied to determine the frequency of the nonpigmented form of the disease. There was an overall incidence of 22%. Fifty percent of all cases had no characteristic pigmentation, if the duration of night vision difficulty was three years or less. The nonpigmented form of retinitis pigmentosa was frequently an early stage of the disease and not an unusual or atypical variant. Patients without the pigmentary changes characteristic of the disorder also showed less functional impairment: the electroretinographic b wave was more apt to be recordable, although impaired, and the rod threshold, as determined by dark adaptation measurement, was less elevated. The clinician should suspect retinitis pigmentosa, even in the absence of pigmentary changes, if there is a family history of the disorder, night blindness, peripheral field loss, and an impaired or nonrecordable electroretinographic response."}
{"id": "PMID:1083674", "title": "Endophthalmitis after vitrectomy.", "content": "A 29-year-old white man with growth-onset diabetes developed fulminant endophthalmitis after vitreous lavage. The only significant symptom was severe pain 36 hours after surgery. The endophthalmitis rapidly became more severe. Despite systemic antibiotics, therapeutic vitrectomy and lensectomy, followed by the intraocular injection of antibiotics 48 hours postoperatively, the eye was lost.", "contents": "Endophthalmitis after vitrectomy. A 29-year-old white man with growth-onset diabetes developed fulminant endophthalmitis after vitreous lavage. The only significant symptom was severe pain 36 hours after surgery. The endophthalmitis rapidly became more severe. Despite systemic antibiotics, therapeutic vitrectomy and lensectomy, followed by the intraocular injection of antibiotics 48 hours postoperatively, the eye was lost."}
{"id": "PMID:1083671", "title": "Chest wall stimulation for temporary suppression of competitive rhythms due to permanent pacemaker malfunction.", "content": "Chest wall stimulation by electrical stimuli was employed to suppress permanent demand (ventricular-inhibited) pacemakers in two patients with competitive ventricular rhythms. The stimuli were nor perceived by either patient, and were continued until surgical intervention was effected. In selected patients with permanent pacemaker malfunction, chest wall stimulation may be appropriate and necessary initial therapy until definitive correction is performed.", "contents": "Chest wall stimulation for temporary suppression of competitive rhythms due to permanent pacemaker malfunction. Chest wall stimulation by electrical stimuli was employed to suppress permanent demand (ventricular-inhibited) pacemakers in two patients with competitive ventricular rhythms. The stimuli were nor perceived by either patient, and were continued until surgical intervention was effected. In selected patients with permanent pacemaker malfunction, chest wall stimulation may be appropriate and necessary initial therapy until definitive correction is performed."}
{"id": "PMID:1083675", "title": "A characterization of human tonsillar lymphocytes after separation from other tonsillar cells in an isokinetic gradient of ficoll in tissue culture medium.", "content": "Purified human tonsillar lymphocytes responded less to mitogentic stimulation than did unseparated tonsillar cells. Their response to mitogens was restored when they were combined with cells from all other gradient fractions. We interpret our data as evidence that the majority of tonsillar lymphocytes require the presence of more rapidly sedimenting cells for a maximum response to the tested mitogens. The purified tonsillar lymphoyctes were 47.0% lymphocytes that have detectable surface immunoglobulin and 29.9% lumphocytes that form rosettes with sheep red blood cells. The predominant cell surface immunoglobulin was IgM. Digestion of the tonsil with trypsin yielded tenfold more plasma cells, more vialbe cells, and a larger proportion of blasts, histiocytes, and binucleated cells than were obtained by mechanical dissociation of the tissue.", "contents": "A characterization of human tonsillar lymphocytes after separation from other tonsillar cells in an isokinetic gradient of ficoll in tissue culture medium. Purified human tonsillar lymphocytes responded less to mitogentic stimulation than did unseparated tonsillar cells. Their response to mitogens was restored when they were combined with cells from all other gradient fractions. We interpret our data as evidence that the majority of tonsillar lymphocytes require the presence of more rapidly sedimenting cells for a maximum response to the tested mitogens. The purified tonsillar lymphoyctes were 47.0% lymphocytes that have detectable surface immunoglobulin and 29.9% lumphocytes that form rosettes with sheep red blood cells. The predominant cell surface immunoglobulin was IgM. Digestion of the tonsil with trypsin yielded tenfold more plasma cells, more vialbe cells, and a larger proportion of blasts, histiocytes, and binucleated cells than were obtained by mechanical dissociation of the tissue."}
{"id": "PMID:1083676", "title": "Interactions of diphenylhydantoin and cardiac glycosides on atrial potassium.", "content": "Effects of diphenylhydantoin (DPH) on amphibian atrial myocardium K were investigated using a method which permits both total tissue K and tension response to be monitored continuously. In normal (nondigitalized) preparations, DPH caused a decrease in average K efflux, a net gain of tissue K, and negativeinotropy at low perfusate K concentrations. However, the DPH-induced gain of tissue K was abolished at high perfusate K concentrations while marked negative inotropy was still observed. It is concluded that a gain of tissue K is not the cause of DPH-induced negative inotropy. When digitalis-induced inotropy was associated with tissue K loss, DPH reversed tissue K loss and positive inotropy and caused a decrease in average K efflux. In the presence of toxic effects of digitalis, DPH reversed the K loss and the contracture, but the loss of developed tension was not reversed by DPH. Transmembrane resting potentials and action potential duration were reduced by digitalis and were returned to or above control levels in the presence of DPH. The present findings are consistent with the hypothesis that the therapeutic effect of DPH in digitalis toxicity is brought about by an inhibition of K efflux. This would tend to minimize the loss of tissue K which results from sodium pump inhibition.", "contents": "Interactions of diphenylhydantoin and cardiac glycosides on atrial potassium. Effects of diphenylhydantoin (DPH) on amphibian atrial myocardium K were investigated using a method which permits both total tissue K and tension response to be monitored continuously. In normal (nondigitalized) preparations, DPH caused a decrease in average K efflux, a net gain of tissue K, and negativeinotropy at low perfusate K concentrations. However, the DPH-induced gain of tissue K was abolished at high perfusate K concentrations while marked negative inotropy was still observed. It is concluded that a gain of tissue K is not the cause of DPH-induced negative inotropy. When digitalis-induced inotropy was associated with tissue K loss, DPH reversed tissue K loss and positive inotropy and caused a decrease in average K efflux. In the presence of toxic effects of digitalis, DPH reversed the K loss and the contracture, but the loss of developed tension was not reversed by DPH. Transmembrane resting potentials and action potential duration were reduced by digitalis and were returned to or above control levels in the presence of DPH. The present findings are consistent with the hypothesis that the therapeutic effect of DPH in digitalis toxicity is brought about by an inhibition of K efflux. This would tend to minimize the loss of tissue K which results from sodium pump inhibition."}
{"id": "PMID:1083677", "title": "Efficacy of selective splanchnic arteriography and vasopressin perfusion in diagnosis and treatment of gastrointestinal hemorrhage.", "content": "Vasopressin infusion initially controlled 80 per cent of patients bleeding from portal hypertension, and 53 per cent did not rebleed after removal of the catheter. This figure is significantly greater than the 28 per cent of patients totally controlled by esophageal tamponade (p less than 0.075). Similar rates of success were achieved by vasopressin infusion for gastric, duodenal, and colonic bleeding sites. These results suggest that visceral arterial infusion of vasopressin is the method of choice for the short-term therapeutic management of massive gastrointestinal bleeding from portal hypertension. Vasopressin infusion also appears to be a valuable means of treating patients with massive gastrointestinal bleeding secondary to shallow gastric ulcers, gastritis, Mallory-Weiss tears, colonic bleeding and \"poor risk\" patients with deep gastric, marginal, or duodenal ulcers when conventional medical therapy has failed. The presence of a coagulation abnormality in patients with portal hypertension significantly reduced the complete control of bleeding to only 27 per cent ( p less than 0.010) and survival rate to 14 per cent (p less than 0.050). Visceral arterial perfusion proved to be an effective means of arresting hemorrhage, but the overall improvement in hospital mortality in this group of poor risk patients remains unproved.", "contents": "Efficacy of selective splanchnic arteriography and vasopressin perfusion in diagnosis and treatment of gastrointestinal hemorrhage. Vasopressin infusion initially controlled 80 per cent of patients bleeding from portal hypertension, and 53 per cent did not rebleed after removal of the catheter. This figure is significantly greater than the 28 per cent of patients totally controlled by esophageal tamponade (p less than 0.075). Similar rates of success were achieved by vasopressin infusion for gastric, duodenal, and colonic bleeding sites. These results suggest that visceral arterial infusion of vasopressin is the method of choice for the short-term therapeutic management of massive gastrointestinal bleeding from portal hypertension. Vasopressin infusion also appears to be a valuable means of treating patients with massive gastrointestinal bleeding secondary to shallow gastric ulcers, gastritis, Mallory-Weiss tears, colonic bleeding and \"poor risk\" patients with deep gastric, marginal, or duodenal ulcers when conventional medical therapy has failed. The presence of a coagulation abnormality in patients with portal hypertension significantly reduced the complete control of bleeding to only 27 per cent ( p less than 0.010) and survival rate to 14 per cent (p less than 0.050). Visceral arterial perfusion proved to be an effective means of arresting hemorrhage, but the overall improvement in hospital mortality in this group of poor risk patients remains unproved."}
{"id": "PMID:1083679", "title": "The central vascular pattern of the eyeground in children with drusen of the optic disk.", "content": "In a series of 50 children with optic disk drusen the anomaly was found to be associated with unusual properties. A cilioretinal artery was more frequent in the series with drusen than in a control series of the same age. The emergence and course of the central retinal vessels were studied in the right-sided fundi of 46 of the children and in two control series (the fundi of the \"better\" eyes of 46 strabismic children and the fundi of one of the eyes of 10 children or young adults with papilledema). Analysis of the measurements indicated that early branching of the central vessels and vascular tortuosity are so frequently associated with optic disk drusen that they can be considered features of the anomaly. On the other hand, the fundal vascular features in papilledema seem to be distinguishable by the method used from those associated with optic disk drusen. When the peripapillary choroidal vascular pattern was studied by fluorescein angiography, choriocapillaris filling was delayed in about the half of the angiograms. The aberrant vascular features found on and around optic disks with drusen can be explained by an embryonic affection of the developing vascular system at the optic nerve head. In this respect the anomaly seems to resemble congenital disorders of mesodermal origin.", "contents": "The central vascular pattern of the eyeground in children with drusen of the optic disk. In a series of 50 children with optic disk drusen the anomaly was found to be associated with unusual properties. A cilioretinal artery was more frequent in the series with drusen than in a control series of the same age. The emergence and course of the central retinal vessels were studied in the right-sided fundi of 46 of the children and in two control series (the fundi of the \"better\" eyes of 46 strabismic children and the fundi of one of the eyes of 10 children or young adults with papilledema). Analysis of the measurements indicated that early branching of the central vessels and vascular tortuosity are so frequently associated with optic disk drusen that they can be considered features of the anomaly. On the other hand, the fundal vascular features in papilledema seem to be distinguishable by the method used from those associated with optic disk drusen. When the peripapillary choroidal vascular pattern was studied by fluorescein angiography, choriocapillaris filling was delayed in about the half of the angiograms. The aberrant vascular features found on and around optic disks with drusen can be explained by an embryonic affection of the developing vascular system at the optic nerve head. In this respect the anomaly seems to resemble congenital disorders of mesodermal origin."}
{"id": "PMID:1083680", "title": "[The disjunction zone in the slit-image-photograph of the human aging lens (author's transl)].", "content": "Standardised slit-image-photographs of 178 clear lenses were evaluated by microdensitometry. The mean distance of the disjunction zone from the anterior capsule and the density of the disjunction zone remain constant during life. Its thickness will be reduced and its extension into deeper layers increases with age. In contrast to these findings the thickness of the whole lens according to the increasing thickness of the cortex increases with age. Its density increases too in a not linear manner probably on behalf of the increasing number of reflecting zones (Alterskernstreifen). The density of the nucleus of the lens increases with age, whereas its densitometrically defined thickness remains constant during life. The disjunction zone behaves independently from age in contrast to other parts of the lens.", "contents": "[The disjunction zone in the slit-image-photograph of the human aging lens (author's transl)]. Standardised slit-image-photographs of 178 clear lenses were evaluated by microdensitometry. The mean distance of the disjunction zone from the anterior capsule and the density of the disjunction zone remain constant during life. Its thickness will be reduced and its extension into deeper layers increases with age. In contrast to these findings the thickness of the whole lens according to the increasing thickness of the cortex increases with age. Its density increases too in a not linear manner probably on behalf of the increasing number of reflecting zones (Alterskernstreifen). The density of the nucleus of the lens increases with age, whereas its densitometrically defined thickness remains constant during life. The disjunction zone behaves independently from age in contrast to other parts of the lens."}
{"id": "PMID:1083681", "title": "[The disjunction zone in the slit-image-photograph of the human lens in eye diseases (author's transl)].", "content": "Standardised slit-image-photographs of the clear human lens in 78 eyes with wide angle glaucoma, 29 eyes with uveitis and 35 eyes after an operation of a retinal detachment were evaluated by microdensitometry and compared with clear lenses of 178 eyes without eye diseases. Most significant alterations were observed in the anterior disjunction zone. Its thickness was increased in all these diseases with the exception of the glaucoma eyes treated with derivates of phenylephrine and/or cholinesteraseinhibitors. Under these conditions the disjunction zone disappeared. The alterations of the lens cortex and of the nucleus of the lens with respect to the thickness and its density were mostly accelerations of the normal aging process and only glaucoma eyes were partly in contrast to this process. Thickening of the anterior disjunction zone may be combined with a thickening of the whole lens and vice versa.", "contents": "[The disjunction zone in the slit-image-photograph of the human lens in eye diseases (author's transl)]. Standardised slit-image-photographs of the clear human lens in 78 eyes with wide angle glaucoma, 29 eyes with uveitis and 35 eyes after an operation of a retinal detachment were evaluated by microdensitometry and compared with clear lenses of 178 eyes without eye diseases. Most significant alterations were observed in the anterior disjunction zone. Its thickness was increased in all these diseases with the exception of the glaucoma eyes treated with derivates of phenylephrine and/or cholinesteraseinhibitors. Under these conditions the disjunction zone disappeared. The alterations of the lens cortex and of the nucleus of the lens with respect to the thickness and its density were mostly accelerations of the normal aging process and only glaucoma eyes were partly in contrast to this process. Thickening of the anterior disjunction zone may be combined with a thickening of the whole lens and vice versa."}
{"id": "PMID:1083682", "title": "Treatment of Coats' disease with photocoagulation.", "content": "71 eyes with Coats' disease were treated with photocoagulation. By coagulation of the abnormal vessels the disease process could be halted in 64 eyes over a mean observation time of 2 years and 3 months after completion of treatment. In 51 of these eyes the lipoid deposits regressed. In only 7 eyes a progression of the disease could not be prevented. Complications of photocoagulation treatment were not observed.", "contents": "Treatment of Coats' disease with photocoagulation. 71 eyes with Coats' disease were treated with photocoagulation. By coagulation of the abnormal vessels the disease process could be halted in 64 eyes over a mean observation time of 2 years and 3 months after completion of treatment. In 51 of these eyes the lipoid deposits regressed. In only 7 eyes a progression of the disease could not be prevented. Complications of photocoagulation treatment were not observed."}
{"id": "PMID:1083683", "title": "[Experimental fluorescein angiographic and microscopic investigations on laser treated rat eyes (author's transl)].", "content": "On fundi of pigmented rats, with Argon laser constantly 300 mW of power. 2 seconds exposure time and 50 microns spot diameter, coagulations have been performed. The eyes have been observed up till 3 months after the intervention, then angiographies were performed, and the following enucleated eyes have been examined with the fluorescence microscope. Neovascularizations and dye diffusions were observable mainly in the marginal zones of the arised cicatrices. The neovascularizations were starting from the choroid and penetrating into the outer retinal layers.", "contents": "[Experimental fluorescein angiographic and microscopic investigations on laser treated rat eyes (author's transl)]. On fundi of pigmented rats, with Argon laser constantly 300 mW of power. 2 seconds exposure time and 50 microns spot diameter, coagulations have been performed. The eyes have been observed up till 3 months after the intervention, then angiographies were performed, and the following enucleated eyes have been examined with the fluorescence microscope. Neovascularizations and dye diffusions were observable mainly in the marginal zones of the arised cicatrices. The neovascularizations were starting from the choroid and penetrating into the outer retinal layers."}
{"id": "PMID:1083684", "title": "[Demonstration of aqueous outflow patterns of normal and glaucomatous human eyes through the injection of fluorescein solution in the anterior chamber (author's transl)].", "content": "After partially injecting the anterior chamber with a 0.2% fluorescein-Ringer solution we examined using a blue filter slitlamp normal eyes, eyes with open-angle glaucoma, and eyes following iridectomy, iridencleisis, Elliot's trephination, and trabeculectomy. In normal eyes at physiological intraocular pressure, 1--9 aqueous veins were found with a mean of 4.6. The distribution of these veins was usually irregular, being more numerous and of largest caliber in the lower nasal quadrant. With only a few exceptions the aqueous veins originated 1--2 1/2 mm peripheral from the limbus and joined the episcleral veins after a short course. The venae recipientes were characterized by a straight and deep course. In eyes with an open-angle glaucoma the average number of aqueous veins was found to be increased compared to the number found in healthy eyes. However, the caliber of these veins was smaller and distribution mor uniform. Eyes having undergone iridectomy do not differ from normal eyes in the mechanism of aqueous humor outflow. After iridencleisis and after trephination the transport of aqueous humor from the anterior chamber is achieved through a filtration bleb: (1) transconjunctival, (2) by bulk-flow through lymphatic vessels, (3) diffusely through lymphatic vessels or veins. In most cases the different transport mechanisms were combined and pure types were seldom found. There was a very definite relationship found between the size of the filtration bleb and the development of draining lymphatic vessels. After trabeculectomy the following drainage mechanisms of the aqueous humor were observed: 1. Subconjunctival outflow of aqueous humor. The further drainage in this case was achieved as in the old types of fistula building operations i.e., primarily through the lymphatic vessels. 2. Direct transport of aqueous humor into the surgical area through newly incorporated veins and lymphatic vessels. 3. Drainage of aqueous humor through still functioning aqueous veins.", "contents": "[Demonstration of aqueous outflow patterns of normal and glaucomatous human eyes through the injection of fluorescein solution in the anterior chamber (author's transl)]. After partially injecting the anterior chamber with a 0.2% fluorescein-Ringer solution we examined using a blue filter slitlamp normal eyes, eyes with open-angle glaucoma, and eyes following iridectomy, iridencleisis, Elliot's trephination, and trabeculectomy. In normal eyes at physiological intraocular pressure, 1--9 aqueous veins were found with a mean of 4.6. The distribution of these veins was usually irregular, being more numerous and of largest caliber in the lower nasal quadrant. With only a few exceptions the aqueous veins originated 1--2 1/2 mm peripheral from the limbus and joined the episcleral veins after a short course. The venae recipientes were characterized by a straight and deep course. In eyes with an open-angle glaucoma the average number of aqueous veins was found to be increased compared to the number found in healthy eyes. However, the caliber of these veins was smaller and distribution mor uniform. Eyes having undergone iridectomy do not differ from normal eyes in the mechanism of aqueous humor outflow. After iridencleisis and after trephination the transport of aqueous humor from the anterior chamber is achieved through a filtration bleb: (1) transconjunctival, (2) by bulk-flow through lymphatic vessels, (3) diffusely through lymphatic vessels or veins. In most cases the different transport mechanisms were combined and pure types were seldom found. There was a very definite relationship found between the size of the filtration bleb and the development of draining lymphatic vessels. After trabeculectomy the following drainage mechanisms of the aqueous humor were observed: 1. Subconjunctival outflow of aqueous humor. The further drainage in this case was achieved as in the old types of fistula building operations i.e., primarily through the lymphatic vessels. 2. Direct transport of aqueous humor into the surgical area through newly incorporated veins and lymphatic vessels. 3. Drainage of aqueous humor through still functioning aqueous veins."}
{"id": "PMID:1083685", "title": "[The combination of micro-disk-electrophoresis and electroimmunodiffusion for investigation of aqueous humor proteins (author's transl)].", "content": "A combination of micro-disk-electrophoresis and electrophoresis into antibody-containing agarose gel is described which permits both qualitative and quantitative analysis of human aqueous humor proteins. This technique is an enrichment of methods for investigation of aqueous humor.", "contents": "[The combination of micro-disk-electrophoresis and electroimmunodiffusion for investigation of aqueous humor proteins (author's transl)]. A combination of micro-disk-electrophoresis and electrophoresis into antibody-containing agarose gel is described which permits both qualitative and quantitative analysis of human aqueous humor proteins. This technique is an enrichment of methods for investigation of aqueous humor."}
{"id": "PMID:1083686", "title": "[Experimental siderosis bulbi: an echographic-histological study (author's transl)].", "content": "In 20 eyes of 10 rabbits a siderosis was provoked by intravitreal injection of 0,1 ml of a 1% watery solution of Mohr's salt. The course of the following vitreous retraction was observed during 4 weeks by means of ultrasonography, and each echogram was compared with the corresponding histological finding. The results of these investigations show that by means of ultrasonography vitreous destruction or liquefaction can be ascertained and distinguished from an undulation, taught or funnel-shaped detachment of the posterior hyaloid membrane; in addition, this membrane and retinal detachments are well discernible by this method.", "contents": "[Experimental siderosis bulbi: an echographic-histological study (author's transl)]. In 20 eyes of 10 rabbits a siderosis was provoked by intravitreal injection of 0,1 ml of a 1% watery solution of Mohr's salt. The course of the following vitreous retraction was observed during 4 weeks by means of ultrasonography, and each echogram was compared with the corresponding histological finding. The results of these investigations show that by means of ultrasonography vitreous destruction or liquefaction can be ascertained and distinguished from an undulation, taught or funnel-shaped detachment of the posterior hyaloid membrane; in addition, this membrane and retinal detachments are well discernible by this method."}
{"id": "PMID:1083687", "title": "[Experiences in the radiographic contrast medium dimer-X in orbital diagnosis (author's transl)].", "content": "Experiments in animals and the employment of the contrast medium in a number of patients showed, that Dimer-X is well tolerated. No negative side effects were observed during the study. Employing only a little quantity of the medium, diluted with distilled water in the ratio of 1:3 or 1:4, good contrast representations are obtainable in the tissue of the orbits. It is completely absorbed within a relatively short period.", "contents": "[Experiences in the radiographic contrast medium dimer-X in orbital diagnosis (author's transl)]. Experiments in animals and the employment of the contrast medium in a number of patients showed, that Dimer-X is well tolerated. No negative side effects were observed during the study. Employing only a little quantity of the medium, diluted with distilled water in the ratio of 1:3 or 1:4, good contrast representations are obtainable in the tissue of the orbits. It is completely absorbed within a relatively short period."}
{"id": "PMID:1083688", "title": "[The influence of the retina on the maturation of the testicles: an experimental study in rabbits (author's transl)].", "content": "In the present experimental study in rabbits the importance of the non-illuminated retina as a superior regulating centre for the maturation of the testicles is demonstrated. Involvement of the retinal maturation by an experimentally provoked total retinal detachment is followed by a cessation of the development of the testicles. The extent of this influence can be brought in evidence by means of the quotation of the fatty acids C20:4/C22:5 as well as on the hand of the histological findings.", "contents": "[The influence of the retina on the maturation of the testicles: an experimental study in rabbits (author's transl)]. In the present experimental study in rabbits the importance of the non-illuminated retina as a superior regulating centre for the maturation of the testicles is demonstrated. Involvement of the retinal maturation by an experimentally provoked total retinal detachment is followed by a cessation of the development of the testicles. The extent of this influence can be brought in evidence by means of the quotation of the fatty acids C20:4/C22:5 as well as on the hand of the histological findings."}
{"id": "PMID:1083689", "title": "[Comparative intraocular pressure measurements with position independent hand-applanatation tonometers (author's transl)].", "content": "A study of comparative intraocular pressure measurements was made using the hand-held applanation tonometers of Perkins and Draeger in the sitting and lying patient. A series of 100 patients was investigated by two observers taking the pressures with both tonometers under blind conditions. The statistical evaluation of the data revealed an average pressure increment when lying down of 2.5 to 3 mm Hg for both observers and both tonometers. This postural response is fully reversible when getting up and an overshoot-phenomenon statistically significant is noted. The pressure increment when lying down is not related to initial pressure level and there is no clear relationship to the age of the patients tested. Interobserver variation as well as variations of both tonometers are evaluated. The effect of repeated tonometry in this study is considered. Some aspects of position influence on the intraocular pressure are discussed.", "contents": "[Comparative intraocular pressure measurements with position independent hand-applanatation tonometers (author's transl)]. A study of comparative intraocular pressure measurements was made using the hand-held applanation tonometers of Perkins and Draeger in the sitting and lying patient. A series of 100 patients was investigated by two observers taking the pressures with both tonometers under blind conditions. The statistical evaluation of the data revealed an average pressure increment when lying down of 2.5 to 3 mm Hg for both observers and both tonometers. This postural response is fully reversible when getting up and an overshoot-phenomenon statistically significant is noted. The pressure increment when lying down is not related to initial pressure level and there is no clear relationship to the age of the patients tested. Interobserver variation as well as variations of both tonometers are evaluated. The effect of repeated tonometry in this study is considered. Some aspects of position influence on the intraocular pressure are discussed."}
{"id": "PMID:1083690", "title": "Glycogen concentration changes in retina, vitreous body and other eye tissues caused by disturbances of blood circulation.", "content": "The glycogen content in the individual eye tissues is strongly correlated to blood supply. Our investigations on the retina of bovines, which have not been fully evaluated, show that the time interval between interruption of blood supply and preparation of the retina is of special importance. Pressure ischemia affects a decrease in glycogen content in the retina and vitreous of rabbits, which is, however, less distinct in the vitreous. Decrease of glycogen with ischemia also takes place in the cornea and, to a lesser degree, in iris and choroid. In contrast, there is no decrease in the glycogen content of the lens. Changes in glycogen content of the rabbit retina after ligation of the A. carotis communis is less distinct than with pressure ischemia. In the vitreous, changes in glycogen content could not be observed. Values measured in both tissues of the ligated eye decrease with additional pressure ischemia.", "contents": "Glycogen concentration changes in retina, vitreous body and other eye tissues caused by disturbances of blood circulation. The glycogen content in the individual eye tissues is strongly correlated to blood supply. Our investigations on the retina of bovines, which have not been fully evaluated, show that the time interval between interruption of blood supply and preparation of the retina is of special importance. Pressure ischemia affects a decrease in glycogen content in the retina and vitreous of rabbits, which is, however, less distinct in the vitreous. Decrease of glycogen with ischemia also takes place in the cornea and, to a lesser degree, in iris and choroid. In contrast, there is no decrease in the glycogen content of the lens. Changes in glycogen content of the rabbit retina after ligation of the A. carotis communis is less distinct than with pressure ischemia. In the vitreous, changes in glycogen content could not be observed. Values measured in both tissues of the ligated eye decrease with additional pressure ischemia."}
{"id": "PMID:1083691", "title": "[Light and electron microscopic studies on short-term preserved corneae (author's transl)].", "content": "Rabbit corneae were stored at a temperature of +4 degrees C in the following media: 1. 5% Dextran T40, 2. McCoy's Medium 5a (modified), 3. Dextran T 40+McCoy's 5a Medium + gentamicin sulphate (250 mug/ml). The endothelia of the corneae were examined by light and electron microscopy after preservation for a varied number of days. The structure of the endothelia, stored for 1, 6, 12, 18 days, was compared to fresh corneal endothelial cells. The results indicate that corneae preserved in Dextran 5% T40 + McCoy's 5a Medium + gentamicin sulphate might be equivalent to fresh corneal tissue in keratoplasties.", "contents": "[Light and electron microscopic studies on short-term preserved corneae (author's transl)]. Rabbit corneae were stored at a temperature of +4 degrees C in the following media: 1. 5% Dextran T40, 2. McCoy's Medium 5a (modified), 3. Dextran T 40+McCoy's 5a Medium + gentamicin sulphate (250 mug/ml). The endothelia of the corneae were examined by light and electron microscopy after preservation for a varied number of days. The structure of the endothelia, stored for 1, 6, 12, 18 days, was compared to fresh corneal endothelial cells. The results indicate that corneae preserved in Dextran 5% T40 + McCoy's 5a Medium + gentamicin sulphate might be equivalent to fresh corneal tissue in keratoplasties."}
{"id": "PMID:1083692", "title": "Studies on the influence of leucomycine on the carbohydrate metabolism of the lens in vitro.", "content": "The influence of leucomycine (10(-5) M--5 X 10(-3) M) on enzyme activities, free adenine nucleotides, and some intermediates of glycolysis in bovine lenses has been investigated. After a 48 h incubation in TCM 199, 6 out of 9 enzymes activities tested were decreased. Concentrations of energy rich nucleotifes, glucose, fructose, and fructose-6-phosphate as well as those of fructose-1,6-diphosphate and lactate were also decreased, whereas adenine monophosphate was increased.", "contents": "Studies on the influence of leucomycine on the carbohydrate metabolism of the lens in vitro. The influence of leucomycine (10(-5) M--5 X 10(-3) M) on enzyme activities, free adenine nucleotides, and some intermediates of glycolysis in bovine lenses has been investigated. After a 48 h incubation in TCM 199, 6 out of 9 enzymes activities tested were decreased. Concentrations of energy rich nucleotifes, glucose, fructose, and fructose-6-phosphate as well as those of fructose-1,6-diphosphate and lactate were also decreased, whereas adenine monophosphate was increased."}
{"id": "PMID:1083693", "title": "[A histological and electron microscopic study of the rabbit retina after application of vincristin (author's transl)].", "content": "Temporary electrophysiological damage to the rabbit eye proportional to dosis and application time was observed after application of Vincristin (Lilly). Cell destruction in all retinal layers was observed upon histological and electron microscopic examination of the animals. The outer segments of the receptor cells showed chiefly diffuse damage, and the pigment epithelial cells showed a high incidence of reorganization. Follow-up examination one year later showed this damage to be partially reparable, especially when very high doses had not been applied. Regeneration of the outer segments was observed. Destruction through pyknosis of the nucleus and cytoplasmal destruction was observed in a small number of receptor cells, bipolar ganglion cells, and ganglion cells. This also explained the absence of lasting functional abnormalities in the ERG and EVP.", "contents": "[A histological and electron microscopic study of the rabbit retina after application of vincristin (author's transl)]. Temporary electrophysiological damage to the rabbit eye proportional to dosis and application time was observed after application of Vincristin (Lilly). Cell destruction in all retinal layers was observed upon histological and electron microscopic examination of the animals. The outer segments of the receptor cells showed chiefly diffuse damage, and the pigment epithelial cells showed a high incidence of reorganization. Follow-up examination one year later showed this damage to be partially reparable, especially when very high doses had not been applied. Regeneration of the outer segments was observed. Destruction through pyknosis of the nucleus and cytoplasmal destruction was observed in a small number of receptor cells, bipolar ganglion cells, and ganglion cells. This also explained the absence of lasting functional abnormalities in the ERG and EVP."}
{"id": "PMID:1083694", "title": "Uptake of horseradish peroxidase (HRP) by the endothelium of the short-term stored rabbit cornea.", "content": "In vitro study of the uptake of horseradish peroxidase (HRP) by the corneal endothelium was carried out on rabbit corneae which were stored in a preserving medium for various periods. The stored corneae were incubated in HRP solution with or without adenosine triphosphate (ATP). The number of pinocytotic vesicles decreased as the storage time was prolonged. Addition of ATP to the HRP solution led to an increase in the pinocytotic vesicles in all storage periods. The pinocytosis in the 12-day stored corneal endothelium was only observed when the tissue was incubated in an HRP solution containing ATP. From these results, it was concluded that the viability of the stored corneae can be evaluated with uptake of HRP by the endothelial cell, and that the addition of ATP leads to an enhancement of pinocytotic activity in the endothelial cell.", "contents": "Uptake of horseradish peroxidase (HRP) by the endothelium of the short-term stored rabbit cornea. In vitro study of the uptake of horseradish peroxidase (HRP) by the corneal endothelium was carried out on rabbit corneae which were stored in a preserving medium for various periods. The stored corneae were incubated in HRP solution with or without adenosine triphosphate (ATP). The number of pinocytotic vesicles decreased as the storage time was prolonged. Addition of ATP to the HRP solution led to an increase in the pinocytotic vesicles in all storage periods. The pinocytosis in the 12-day stored corneal endothelium was only observed when the tissue was incubated in an HRP solution containing ATP. From these results, it was concluded that the viability of the stored corneae can be evaluated with uptake of HRP by the endothelial cell, and that the addition of ATP leads to an enhancement of pinocytotic activity in the endothelial cell."}
{"id": "PMID:1083695", "title": "[Fuchs's heterochromic cyclitis. Electron-microscopic study of nine iris biopsies (author's transl)].", "content": "The irises of 9 cases of Fuchs's heterochromic cyclitis, obtained by iridectomy during cataract surgery, were examined pathohistologically and electron microscopically. There is a chronic type of iridocyclitis with infiltration of mainly plasma cells and with a marked degenerative and atrophic component. The main feature of the fine structure is the presence of isolated organelles, mainly mitochondria, with intact structure, a result of a process which seems to destroy first the endoplasmatic reticulum and leads to cytolysis. This phenomenon, however, is present too in cases of types chronic iridocyclitis, but to a lesser extent. Thus, the extent of the atrophic process seems to be the only pathognomonic criteria for Fuchs's heterochromic cyclitis. The electron microscope cannot reveal pathognomonic criteria for Fuchs's heterochromic cyclitis, and, furthermore, we could not demonstrate any findings which could establish or confirm the etiology. The theory of a dysfunction of the sympathetic innervation and the theory of a vascular pathogenesis could not be confirmed. The question is discussed whether Fuchs's heterochromic cyclitis is a autoimmune-disease which attacks the endoplasmatic reticulum first.", "contents": "[Fuchs's heterochromic cyclitis. Electron-microscopic study of nine iris biopsies (author's transl)]. The irises of 9 cases of Fuchs's heterochromic cyclitis, obtained by iridectomy during cataract surgery, were examined pathohistologically and electron microscopically. There is a chronic type of iridocyclitis with infiltration of mainly plasma cells and with a marked degenerative and atrophic component. The main feature of the fine structure is the presence of isolated organelles, mainly mitochondria, with intact structure, a result of a process which seems to destroy first the endoplasmatic reticulum and leads to cytolysis. This phenomenon, however, is present too in cases of types chronic iridocyclitis, but to a lesser extent. Thus, the extent of the atrophic process seems to be the only pathognomonic criteria for Fuchs's heterochromic cyclitis. The electron microscope cannot reveal pathognomonic criteria for Fuchs's heterochromic cyclitis, and, furthermore, we could not demonstrate any findings which could establish or confirm the etiology. The theory of a dysfunction of the sympathetic innervation and the theory of a vascular pathogenesis could not be confirmed. The question is discussed whether Fuchs's heterochromic cyclitis is a autoimmune-disease which attacks the endoplasmatic reticulum first."}
{"id": "PMID:1083696", "title": "Influence of various anesthetic drugs on the intraocular pressure of cats.", "content": "Cats with one normal eye and one eye with experimental glaucoma were trained to tolerate tonometry while conscious. They were then given the general anesthetic agents pentobarbital, ketamine, and CI-744 in the lowest and twice the lowest dosages which permitted tonometry in untrained cats. Pentobarbital lowered intraocular pressure (IOP) by 20-50%. Ketamine raised IOP by about 10%. CI-744 had essentially no effect on IOP. The two conpoments of CI-744, tiletamine (CI-634) and zolazepam (CI-716) were given individually in the doses in which they had been given as part of CI-744. Tiletamine had no effect on IOP, while zolazepam lowered IOP by about 10%.", "contents": "Influence of various anesthetic drugs on the intraocular pressure of cats. Cats with one normal eye and one eye with experimental glaucoma were trained to tolerate tonometry while conscious. They were then given the general anesthetic agents pentobarbital, ketamine, and CI-744 in the lowest and twice the lowest dosages which permitted tonometry in untrained cats. Pentobarbital lowered intraocular pressure (IOP) by 20-50%. Ketamine raised IOP by about 10%. CI-744 had essentially no effect on IOP. The two conpoments of CI-744, tiletamine (CI-634) and zolazepam (CI-716) were given individually in the doses in which they had been given as part of CI-744. Tiletamine had no effect on IOP, while zolazepam lowered IOP by about 10%."}
{"id": "PMID:1083697", "title": "[The influence of vitamin A deficiency on the electrooculogram (author's transl)].", "content": "Hypovitaminosis A caused by a malabsorption syndrome had led to nearly selective disturbance of rod function in a 30 years old female. No rod dark-adaptation could be recorded. The b-wave in the ERG was lacking and the EOG was flat. The cone function appeared to be unimpaired. This could be documented by normal cone-ERG, colour vision, visual acuity and central visual fields. Following treatment with vitamin A the subjective dark-adaptation, the b-wave in ERG and the EOG recovered. The results show that cones contribute barely to the oscillations of EOG.", "contents": "[The influence of vitamin A deficiency on the electrooculogram (author's transl)]. Hypovitaminosis A caused by a malabsorption syndrome had led to nearly selective disturbance of rod function in a 30 years old female. No rod dark-adaptation could be recorded. The b-wave in the ERG was lacking and the EOG was flat. The cone function appeared to be unimpaired. This could be documented by normal cone-ERG, colour vision, visual acuity and central visual fields. Following treatment with vitamin A the subjective dark-adaptation, the b-wave in ERG and the EOG recovered. The results show that cones contribute barely to the oscillations of EOG."}
{"id": "PMID:1083698", "title": "Ring-shaped alkali burns of the rabbit cornea. III. Late phase of the stroma reaction.", "content": "The present paper deals with late changes of the corneal stroma after alkali burns from the 5th day after the injury up to the 58th day. As has been pointed out in a previous paper, between the 3rd and 5th day the process of healing within the corneal stroma changes and at the end a dense scar ensues.", "contents": "Ring-shaped alkali burns of the rabbit cornea. III. Late phase of the stroma reaction. The present paper deals with late changes of the corneal stroma after alkali burns from the 5th day after the injury up to the 58th day. As has been pointed out in a previous paper, between the 3rd and 5th day the process of healing within the corneal stroma changes and at the end a dense scar ensues."}
{"id": "PMID:1083699", "title": "[Does methylmethacrylate induce cardiovascular complications during alloarthroplastic surgery of the hip joint? (AUTHOR'S TRANSL)].", "content": "Cardiovascular experiments in dogs and rabbits under various conditions demonstrated that monomethylacrylate has no direct cardiac depressant activity. The transient decrease in blood pressure and heart rate of patients receiving endoprostheses and experimental animals is elicited by a vagotropic effect of the methacrylates and can be antagonized by prophylactic application of atropine. Severe cardiovascular incidents involving an acute cor pulmonale are induced via the steep rise in pressure within the bone-marrow canal during the forcing in of the endoprosthetic shafts, leading to intravasation of bone marrow and its thromboplastine like compounds, thus resulting in a partial coagulation. A schedule of simple prophylactic procedures proved to be effective in preventing fatal cardiovascular complications.", "contents": "[Does methylmethacrylate induce cardiovascular complications during alloarthroplastic surgery of the hip joint? (AUTHOR'S TRANSL)]. Cardiovascular experiments in dogs and rabbits under various conditions demonstrated that monomethylacrylate has no direct cardiac depressant activity. The transient decrease in blood pressure and heart rate of patients receiving endoprostheses and experimental animals is elicited by a vagotropic effect of the methacrylates and can be antagonized by prophylactic application of atropine. Severe cardiovascular incidents involving an acute cor pulmonale are induced via the steep rise in pressure within the bone-marrow canal during the forcing in of the endoprosthetic shafts, leading to intravasation of bone marrow and its thromboplastine like compounds, thus resulting in a partial coagulation. A schedule of simple prophylactic procedures proved to be effective in preventing fatal cardiovascular complications."}
{"id": "PMID:1083700", "title": "Protease inhibitors in patients with chronic obstructive pulmonary disease: the alpha-antitrypsin heterozygote controversy.", "content": "A group of 163 patients with chronic obstructive pulmonary disease, from the pulmonary service of a large urban hospital, were evaluated for their protease inhibitor (Pi) type by starch gel and crossed immunoelectrophoresis, for serum concentrations of alpha1-antitrypsin and alpha1-antichymotrypsin, and for pulmonary function. Of the patients with emphysema, 17.8% were of Pi type Z; 50% of these were less than 45 years of age, compared to 13% of those of Pi type M. Of all patients with chronic obstructive pulmonary disease, 4.9% were of Pi type Z; 4.9% of patients were of Pi type MZ (heterozygotes) compared with 1.9% of the control population. There was an increased incidence of chronic obstructive pulmonary disease in persons of Pi type MZ, but no increase in persons of Pi type MS. Concentrations of both alpha1-antitrypsin and alpha1-antichymotrypsin were increased and were correlated. No patient had a deficiency of alpha1-antichymotrypsin.", "contents": "Protease inhibitors in patients with chronic obstructive pulmonary disease: the alpha-antitrypsin heterozygote controversy. A group of 163 patients with chronic obstructive pulmonary disease, from the pulmonary service of a large urban hospital, were evaluated for their protease inhibitor (Pi) type by starch gel and crossed immunoelectrophoresis, for serum concentrations of alpha1-antitrypsin and alpha1-antichymotrypsin, and for pulmonary function. Of the patients with emphysema, 17.8% were of Pi type Z; 50% of these were less than 45 years of age, compared to 13% of those of Pi type M. Of all patients with chronic obstructive pulmonary disease, 4.9% were of Pi type Z; 4.9% of patients were of Pi type MZ (heterozygotes) compared with 1.9% of the control population. There was an increased incidence of chronic obstructive pulmonary disease in persons of Pi type MZ, but no increase in persons of Pi type MS. Concentrations of both alpha1-antitrypsin and alpha1-antichymotrypsin were increased and were correlated. No patient had a deficiency of alpha1-antichymotrypsin."}
{"id": "PMID:1083701", "title": "Effect of human alpha-antitrypsin in papain-induced emphysema in the hamster.", "content": "The possibility that human alpha1-antitrypsin could effectively prevent development of emphysematous lesions produced in hamsters 7 days after exposure to aerosolized papain (3% for 3 hours) was investigated. Pretreatment with intratracheal human alpha1-antitrypsin prevented the appearance of these lesions in a dose-dependent manner. On the other hand, systemic administration of large doses of human alpha1-antitrypsin failed to prevent papain-induced pulmonary lesions, despite a significant increase in serum trypsin inhibitory capacity. These results suggest that intra-alveolar rather than serum concentrations of human alpha1-antitrypsin are critical for preventing the development of pulmonary emphysema in this animal model. It is interesting that although the mechanism by which human alpha1-antitrypsin prevented the papain-induced lesions is unknown, intratracheally administered human alpha1-antitrypsin similarly prevented the development of pulmonary emphysematous lesions induced by 0.1 mg of porcine pancreatic elastase given intratracheally.", "contents": "Effect of human alpha-antitrypsin in papain-induced emphysema in the hamster. The possibility that human alpha1-antitrypsin could effectively prevent development of emphysematous lesions produced in hamsters 7 days after exposure to aerosolized papain (3% for 3 hours) was investigated. Pretreatment with intratracheal human alpha1-antitrypsin prevented the appearance of these lesions in a dose-dependent manner. On the other hand, systemic administration of large doses of human alpha1-antitrypsin failed to prevent papain-induced pulmonary lesions, despite a significant increase in serum trypsin inhibitory capacity. These results suggest that intra-alveolar rather than serum concentrations of human alpha1-antitrypsin are critical for preventing the development of pulmonary emphysema in this animal model. It is interesting that although the mechanism by which human alpha1-antitrypsin prevented the papain-induced lesions is unknown, intratracheally administered human alpha1-antitrypsin similarly prevented the development of pulmonary emphysematous lesions induced by 0.1 mg of porcine pancreatic elastase given intratracheally."}
{"id": "PMID:1083702", "title": "Etiologic diagnosis of intrapleural empyema by counterimmunoelectrophoresis.", "content": "Cultural methods failed to identify the infecting organism in 4 patients with intrapleural empyema. Antimicrobial drugs had been administered to 3 of the patients before their admssion to the hospital. In each case, soluble polysaccharides believed to be those of pneumococcus or hemophilus were detected in the empyema fluid by counterimmunoelectrophoresis. These findings provided a rational basis for management of antimicrobial therapy for 3 of the patients and useful information on the origins of the empyema in the fourth patient. Counterimmunoelectrophoresis is a useful additional method for presumptively identifying the infecting organism in patients with intrapleural empyema.", "contents": "Etiologic diagnosis of intrapleural empyema by counterimmunoelectrophoresis. Cultural methods failed to identify the infecting organism in 4 patients with intrapleural empyema. Antimicrobial drugs had been administered to 3 of the patients before their admssion to the hospital. In each case, soluble polysaccharides believed to be those of pneumococcus or hemophilus were detected in the empyema fluid by counterimmunoelectrophoresis. These findings provided a rational basis for management of antimicrobial therapy for 3 of the patients and useful information on the origins of the empyema in the fourth patient. Counterimmunoelectrophoresis is a useful additional method for presumptively identifying the infecting organism in patients with intrapleural empyema."}
{"id": "PMID:1083703", "title": "Upper gastrointestinal bleeding associated with biliary diversion.", "content": "Significant upper gastrointestinal bleeding following operation was noted in 14 of 218 patients undergoing external biliary drainage. None of these patients sustained hepatic injury or needed hepatic resection. Three patients died from causes related to the upper gastrointestinal hemorrhage, and in 12 of the 14 patients the episode of hemorrhage occurred within three days of the biliary tract operation. The majority of the patients were febrile or septic at the time of the biliary operation. Biliary tract infection, when combined with external biliary drainage, may predipose to major upper gastrointestinal hemorrhage.", "contents": "Upper gastrointestinal bleeding associated with biliary diversion. Significant upper gastrointestinal bleeding following operation was noted in 14 of 218 patients undergoing external biliary drainage. None of these patients sustained hepatic injury or needed hepatic resection. Three patients died from causes related to the upper gastrointestinal hemorrhage, and in 12 of the 14 patients the episode of hemorrhage occurred within three days of the biliary tract operation. The majority of the patients were febrile or septic at the time of the biliary operation. Biliary tract infection, when combined with external biliary drainage, may predipose to major upper gastrointestinal hemorrhage."}
{"id": "PMID:1083704", "title": "Primary appendectomy.", "content": "A review of 403 patients undergoing appendectomy in a community hospital led to the following conclusions. Appendectomy with the finding of a normal appendix and no other condition requiring operation was accompanied by a higher complication rate and longer hospitalization than appendectomy for nonperforated diseased appendix. A high frequency of removal of normal appendices did not result in a lower incidence of appendiceal perforation. An increased diagnostic accuracy in the surgical treatment of \"acute appendicitis\" was not associated with increased incidence of appendiceal perforation. These findings support the thesis that a more discriminate diagnosis of acute appendicitis will lower the incidence of unnecessary appendectomy without increasing the morbidity from unrecognized appendiceal disease.", "contents": "Primary appendectomy. A review of 403 patients undergoing appendectomy in a community hospital led to the following conclusions. Appendectomy with the finding of a normal appendix and no other condition requiring operation was accompanied by a higher complication rate and longer hospitalization than appendectomy for nonperforated diseased appendix. A high frequency of removal of normal appendices did not result in a lower incidence of appendiceal perforation. An increased diagnostic accuracy in the surgical treatment of \"acute appendicitis\" was not associated with increased incidence of appendiceal perforation. These findings support the thesis that a more discriminate diagnosis of acute appendicitis will lower the incidence of unnecessary appendectomy without increasing the morbidity from unrecognized appendiceal disease."}
{"id": "PMID:1083705", "title": "Intussusception following jejunoileal bypass: case report and review of the literature.", "content": "Jejunoileal bypass carries a number of potential complications, one of which is intussusception of the excluded segment. The incidence of this complication is probably about one to two per cent. The usual symptoms consist of intermittent episodes of colicky abdominal pain. The roentgenographic diagnosis is difficult because the condition occurs at the blind end of the excluded segment and it is not possible to reflux contrast material to this area. If the intussusception is associated with severe gastrointestinal hemorrhage, selective angiography may provide the diagnosis.", "contents": "Intussusception following jejunoileal bypass: case report and review of the literature. Jejunoileal bypass carries a number of potential complications, one of which is intussusception of the excluded segment. The incidence of this complication is probably about one to two per cent. The usual symptoms consist of intermittent episodes of colicky abdominal pain. The roentgenographic diagnosis is difficult because the condition occurs at the blind end of the excluded segment and it is not possible to reflux contrast material to this area. If the intussusception is associated with severe gastrointestinal hemorrhage, selective angiography may provide the diagnosis."}
{"id": "PMID:1083707", "title": "Night blindness and the retinal mechanism of visual adaptation.", "content": "An account is given of investigations into the mechanisms of dark-adaptation in the retina of man and of the skate and other fish. Working hypotheses as to the possible sites of abnormal function in the various disorders of which night blindness is a feature are presented.", "contents": "Night blindness and the retinal mechanism of visual adaptation. An account is given of investigations into the mechanisms of dark-adaptation in the retina of man and of the skate and other fish. Working hypotheses as to the possible sites of abnormal function in the various disorders of which night blindness is a feature are presented."}
{"id": "PMID:1083706", "title": "The temporal bone in the preauricular pit, cervical fistula, hearing loss syndrome.", "content": "Histological study of the temporal bones of an infant with the preauricular pit, cervical fistula, hearing loss syndrome revealed abnormalities in the middle ear, the vestibular system, and the cochlea. There is a gross bilateral abnormality in the form and relationship of the middle ear spaces, the middle cranial fossa and the inner ear. The horizontal canal lacks ampulla and crista; the posterior canal terminates a short distance from the ampulla. The cochlear cavity is approximately four fifths of normal size. The modiolus of the cochlea shows minor abnormalities. Spiral ganglion cells and peripheral nerve fibers are present in all coils, but are below normal in population in the basal and apical coils. The stria vascularis is slightly deformed and partly atrophic in the upper apical coil. An occasional concretion is present in the base of the stria vascularis in the middle and apical coils.", "contents": "The temporal bone in the preauricular pit, cervical fistula, hearing loss syndrome. Histological study of the temporal bones of an infant with the preauricular pit, cervical fistula, hearing loss syndrome revealed abnormalities in the middle ear, the vestibular system, and the cochlea. There is a gross bilateral abnormality in the form and relationship of the middle ear spaces, the middle cranial fossa and the inner ear. The horizontal canal lacks ampulla and crista; the posterior canal terminates a short distance from the ampulla. The cochlear cavity is approximately four fifths of normal size. The modiolus of the cochlea shows minor abnormalities. Spiral ganglion cells and peripheral nerve fibers are present in all coils, but are below normal in population in the basal and apical coils. The stria vascularis is slightly deformed and partly atrophic in the upper apical coil. An occasional concretion is present in the base of the stria vascularis in the middle and apical coils."}
{"id": "PMID:1083710", "title": "Complications in acute pancreatitis.", "content": "In a group of 260 non-selected cases of acute or subacute pancreatitis, severe complications occurred in 60 (23.1%). Long lasting shock and/or massive internal bleeding (5.4%), severe renal problems (anuria, tubular necrosis, nephrosis) (5.4%) and frank hepatic failure due to extensive liver necrosis or other severe destruction (5.0%), invariably lead to death. The clinical group of findings pointing to a fatal course usually manifested themselves during the first three days. Severe renal and hepatic lesions were in many cases secondary to shock in fulminant rapidly deteriorating cases. Prevention and efficient management of shock are thus essential prerequisites for saving the patient. Other important complications included severe intra-abdominal suppuration and abscesses, peritonitis and sepsis (3.9%), pseudocysts of the pancreas (5.4%) and biliary stasis (18.4%). Severe obstruction to bile flow with associated jaundice occurred in only 4.6% of cases; unselected operative biliary decompression does not therefore appear indicated. If an early laparotomy is performed, efficient debridement and drainage are of utmost importance. Fatal pancreatitis was associated with extensive necrosis of the pancreas in about 80% of cases; possibly subtotal pancreatic resection at an early laparotomy would have given better results in these most severe cases, as recently reported in the literature.", "contents": "Complications in acute pancreatitis. In a group of 260 non-selected cases of acute or subacute pancreatitis, severe complications occurred in 60 (23.1%). Long lasting shock and/or massive internal bleeding (5.4%), severe renal problems (anuria, tubular necrosis, nephrosis) (5.4%) and frank hepatic failure due to extensive liver necrosis or other severe destruction (5.0%), invariably lead to death. The clinical group of findings pointing to a fatal course usually manifested themselves during the first three days. Severe renal and hepatic lesions were in many cases secondary to shock in fulminant rapidly deteriorating cases. Prevention and efficient management of shock are thus essential prerequisites for saving the patient. Other important complications included severe intra-abdominal suppuration and abscesses, peritonitis and sepsis (3.9%), pseudocysts of the pancreas (5.4%) and biliary stasis (18.4%). Severe obstruction to bile flow with associated jaundice occurred in only 4.6% of cases; unselected operative biliary decompression does not therefore appear indicated. If an early laparotomy is performed, efficient debridement and drainage are of utmost importance. Fatal pancreatitis was associated with extensive necrosis of the pancreas in about 80% of cases; possibly subtotal pancreatic resection at an early laparotomy would have given better results in these most severe cases, as recently reported in the literature."}
{"id": "PMID:1083711", "title": "Spheroplasts of Haemophilus influenzae induced by cell wall-active antibiotics and their effect upon the interpretation of susceptibility tests.", "content": "The interpretation of in vitro susceptibility tests of Haemophilus influenzae performed by the agar or broth dilution methods with Levinthal enrichment was found to be markedly influenced by the production of spheroplasts by this species. Using an inoculum of 10(7) organisms/ml, this phenomenon was frequently evident macroscopically as a haziness on agar substrates and in broths containing cell wall-acting agents, such as ampicillin, cephalothin, and penicillin, but was not noted with chloramphenicol. Phase-contrast microscopic examination of the haze from these sources revealed numerous spherical bodies in contrast to the typical cocco-bacillary forms observed in growth controls. With this inoculum size, minimal bactericidal concentrations could not be determined since subculture of 0.1 ml of the hazy broths or the surface haze onto chocolate agar resulted in most instances in the development of a small number of colonies which, upon smear and gram stain, revealed typical Haemophilus morphology. An inoculum of 10(4) organisms/ml abolished the haziness on agar surfaces and in broths and resulted in clear-cut end points. Also, although spherical bodies were still present, they were distinctly less in number as contrasted to tests performed with an inoculum of 10(7) organisms/ml. It is recommended that minimal inhibitory concentration end points in antibiotic susceptibility tests be determined by microscopic, rather than macroscopic, observation of the growth milieu to determine the presence or absence of morphologically typical bacilli which, when observed, is indicative of true in vitro resistance.", "contents": "Spheroplasts of Haemophilus influenzae induced by cell wall-active antibiotics and their effect upon the interpretation of susceptibility tests. The interpretation of in vitro susceptibility tests of Haemophilus influenzae performed by the agar or broth dilution methods with Levinthal enrichment was found to be markedly influenced by the production of spheroplasts by this species. Using an inoculum of 10(7) organisms/ml, this phenomenon was frequently evident macroscopically as a haziness on agar substrates and in broths containing cell wall-acting agents, such as ampicillin, cephalothin, and penicillin, but was not noted with chloramphenicol. Phase-contrast microscopic examination of the haze from these sources revealed numerous spherical bodies in contrast to the typical cocco-bacillary forms observed in growth controls. With this inoculum size, minimal bactericidal concentrations could not be determined since subculture of 0.1 ml of the hazy broths or the surface haze onto chocolate agar resulted in most instances in the development of a small number of colonies which, upon smear and gram stain, revealed typical Haemophilus morphology. An inoculum of 10(4) organisms/ml abolished the haziness on agar surfaces and in broths and resulted in clear-cut end points. Also, although spherical bodies were still present, they were distinctly less in number as contrasted to tests performed with an inoculum of 10(7) organisms/ml. It is recommended that minimal inhibitory concentration end points in antibiotic susceptibility tests be determined by microscopic, rather than macroscopic, observation of the growth milieu to determine the presence or absence of morphologically typical bacilli which, when observed, is indicative of true in vitro resistance."}
{"id": "PMID:1083712", "title": "Uptake of metronidazole and its effect on viability in trichomonads and Entamoeba invadens under anaerobic and aerobic conditions.", "content": "[(14)C]metronidazole used at the chemotherapeutic concentration of 10 mug/ml is taken up rapidly by the anaerobic protozoa Tritrichomonas foetus, Trichomonas vaginalis, and Entamoeba invadens kept under anaerobic conditions. It can be calculated that within 30 to 60 min the intracellular concentration of the label is 50 to 100 times higher than in the medium. The presence of air markedly suppresses the uptake in the trichomonads and abolishes it in E. invadens. The suppression disappears after anaerobic conditions are established. The rate of uptake in T. foetus is dependent on the concentration of the drug in the range studied (1 to 200 mug/ml). Analysis of double reciprocal plots suggests that the drug enters the cells predominantly or exclusively by diffusion. The major factor driving the uptake is most likely the intracellular biotransformation of the compound. If less than 3 mug of drug per mg of protein is taken up by T. foetus no decrease in viability is observed. Above this level the cytotoxic activity corresponds roughly to the amount accumulated in the cell, irrespective of whether the conditions are anaerobic or aerobic.", "contents": "Uptake of metronidazole and its effect on viability in trichomonads and Entamoeba invadens under anaerobic and aerobic conditions. [(14)C]metronidazole used at the chemotherapeutic concentration of 10 mug/ml is taken up rapidly by the anaerobic protozoa Tritrichomonas foetus, Trichomonas vaginalis, and Entamoeba invadens kept under anaerobic conditions. It can be calculated that within 30 to 60 min the intracellular concentration of the label is 50 to 100 times higher than in the medium. The presence of air markedly suppresses the uptake in the trichomonads and abolishes it in E. invadens. The suppression disappears after anaerobic conditions are established. The rate of uptake in T. foetus is dependent on the concentration of the drug in the range studied (1 to 200 mug/ml). Analysis of double reciprocal plots suggests that the drug enters the cells predominantly or exclusively by diffusion. The major factor driving the uptake is most likely the intracellular biotransformation of the compound. If less than 3 mug of drug per mg of protein is taken up by T. foetus no decrease in viability is observed. Above this level the cytotoxic activity corresponds roughly to the amount accumulated in the cell, irrespective of whether the conditions are anaerobic or aerobic."}
{"id": "PMID:1083713", "title": "Heterogeneity of Staphylococcus aureus enterotoxin B as a function of growth stage: implications for surveillance of foods.", "content": "Staphylococcus aureus was grown in a fermentor under controlled conditions of pH, oxygenation, and temperature, while the higher-molecular-weight products of its growth were continuously removed across ultrafiltration membranes. These products were examined by single and double gel diffusion and immunoelectrophoresis against a variety of available anti-enterotoxin B antisera. All antisera examined were polyvalent for S. aureus antigens. However, two electrophoretically distinct proteins were the major reactants with the antisera. One of these was present in early- to mid-log-phase cultures. After mid-log growth was achieved, both were present but in continuously changing proportions. This observation was repeated with a variety of growth conditions and media. A significant part of the physicochemical heterogeneity of enterotoxin B observed over the past 20 years is thus correlated with the growth phase of the organism. Taken together, these facts are used to argue for a two-step rationale for the detection of food-borne staphylococcal disease: (i) screening for a presumptive hazard by analysis for any antigen, toxin, or enzyme of S. aureus in a foodstuff and (ii) confirmation of the hazard by identifying the presence of an enterotoxin using a combination of physicochemical and serological techniques.", "contents": "Heterogeneity of Staphylococcus aureus enterotoxin B as a function of growth stage: implications for surveillance of foods. Staphylococcus aureus was grown in a fermentor under controlled conditions of pH, oxygenation, and temperature, while the higher-molecular-weight products of its growth were continuously removed across ultrafiltration membranes. These products were examined by single and double gel diffusion and immunoelectrophoresis against a variety of available anti-enterotoxin B antisera. All antisera examined were polyvalent for S. aureus antigens. However, two electrophoretically distinct proteins were the major reactants with the antisera. One of these was present in early- to mid-log-phase cultures. After mid-log growth was achieved, both were present but in continuously changing proportions. This observation was repeated with a variety of growth conditions and media. A significant part of the physicochemical heterogeneity of enterotoxin B observed over the past 20 years is thus correlated with the growth phase of the organism. Taken together, these facts are used to argue for a two-step rationale for the detection of food-borne staphylococcal disease: (i) screening for a presumptive hazard by analysis for any antigen, toxin, or enzyme of S. aureus in a foodstuff and (ii) confirmation of the hazard by identifying the presence of an enterotoxin using a combination of physicochemical and serological techniques."}
{"id": "PMID:1083716", "title": "The management of infected abdominal aortic grafts and graft-enteric fistulas.", "content": "Seven cases of infected aortic grafts or aorto-enteric fistulas following resection of an abdominal aortic aneurysm are reviewed. All cases were treated with axillo-femoral bypass and graft removal. Patients had recurrent fever, chills, and abdominal pain (5 patients), or massive gastrointestinal hemorrhage (2 patients). The two patients with massive gastrointestinal hemorrhage died. Three of the 5 long-term survivors had a recurrence of the retroperitoneal abscess after graft removal; one of these died. One axillo-femoral bypass graft required early thrombectomy. There have been no subsequent problems with any of the grafts in the 2.5 to 4 year followup period. Axillo-femoral bypass immediately before graft removal is the treatment of choice for infected or fistulous aortic grafts. Any delay in graft removal after the onset of symptoms should be avoided.", "contents": "The management of infected abdominal aortic grafts and graft-enteric fistulas. Seven cases of infected aortic grafts or aorto-enteric fistulas following resection of an abdominal aortic aneurysm are reviewed. All cases were treated with axillo-femoral bypass and graft removal. Patients had recurrent fever, chills, and abdominal pain (5 patients), or massive gastrointestinal hemorrhage (2 patients). The two patients with massive gastrointestinal hemorrhage died. Three of the 5 long-term survivors had a recurrence of the retroperitoneal abscess after graft removal; one of these died. One axillo-femoral bypass graft required early thrombectomy. There have been no subsequent problems with any of the grafts in the 2.5 to 4 year followup period. Axillo-femoral bypass immediately before graft removal is the treatment of choice for infected or fistulous aortic grafts. Any delay in graft removal after the onset of symptoms should be avoided."}
{"id": "PMID:1083717", "title": "The management of Hemophilus influenzae, Type B, pericarditis.", "content": "The incidence of Hemophilus influenzae, type B, infections in children has been increasing recently, so the number of cases of pericarditis is likely to rise also. We describe the clinical manifestations and treatment of H. influenzae, type B, pericarditis based on 4 patients and a review of the literature. The most common complication is cardiac tamponade, which requires drainage. All patients should be treated with antibiotics (chloramphenicol, ampicillin) and a drainage procedure. Because of several recently reported cases of subsequent constrictive pericarditis, we recomment anterior interphrenic pericardiectomy both for drainage and to prevent constrictive pericarditis. With appropriate therapy the survival rate should be very high.", "contents": "The management of Hemophilus influenzae, Type B, pericarditis. The incidence of Hemophilus influenzae, type B, infections in children has been increasing recently, so the number of cases of pericarditis is likely to rise also. We describe the clinical manifestations and treatment of H. influenzae, type B, pericarditis based on 4 patients and a review of the literature. The most common complication is cardiac tamponade, which requires drainage. All patients should be treated with antibiotics (chloramphenicol, ampicillin) and a drainage procedure. Because of several recently reported cases of subsequent constrictive pericarditis, we recomment anterior interphrenic pericardiectomy both for drainage and to prevent constrictive pericarditis. With appropriate therapy the survival rate should be very high."}
{"id": "PMID:1083718", "title": "Vein grafts to left-sided coronary arteries: passage through the transverse sinus.", "content": "Vein grafts to left-sided coronary arteries, unlike those to the right coronary artery, do not course and land naturally on an axis parallel to that of the recipient artery. To run such a course, the graft must take off from the aorta in a direction upward and to the left, then loop back toward the left anterior descending, the diagonal, or the circumflex coronary artery. Because of limited space in this area due to the reflection of the pericardium near the left pulmonary artery and left atrial appendage, the vein loop may bend at that point or, when made shorter, may kink at the site of the coronary anastomosis. To avoid the formation of these sharp angulations, the graft may be made to course posteriorly and superiorly into the transverse sinus, behind the main pulmonary artery and the aorta, and may be sutured on the right anterior aspect of the ascending aorta. Following is the description of this technique and its advantages.", "contents": "Vein grafts to left-sided coronary arteries: passage through the transverse sinus. Vein grafts to left-sided coronary arteries, unlike those to the right coronary artery, do not course and land naturally on an axis parallel to that of the recipient artery. To run such a course, the graft must take off from the aorta in a direction upward and to the left, then loop back toward the left anterior descending, the diagonal, or the circumflex coronary artery. Because of limited space in this area due to the reflection of the pericardium near the left pulmonary artery and left atrial appendage, the vein loop may bend at that point or, when made shorter, may kink at the site of the coronary anastomosis. To avoid the formation of these sharp angulations, the graft may be made to course posteriorly and superiorly into the transverse sinus, behind the main pulmonary artery and the aorta, and may be sutured on the right anterior aspect of the ascending aorta. Following is the description of this technique and its advantages."}
{"id": "PMID:1083719", "title": "Sternal osteomyelitis and mediastinitis after open-heart operation: pathogenesis and prevention.", "content": "Sternal osteomyelitis and mediastinitis caused by Pseudomonas cepacia developed in a patient undergoing coronary artery bypass two weeks after the operation. P. cepacia bacteremia from a contaminated pressure transducer had preceded and probably caused the chest infection. While other authors have suggested that postoperative sternal osteomyelitis and mediastinitis result from local wound contamination, this case suggests the importance of bacteremia as a cause of such gram-negative infections. Since patients undergoing open-heart operation are exposed to many sources of bacteremia, prevention of severe postoperative chest infections may depend in large part on careful preoperative evaluation of each patienc antibiotic regimens, and, as shown in this patient, on very thorough periodic review of equipment sterilization and intravascular monitoring practices.", "contents": "Sternal osteomyelitis and mediastinitis after open-heart operation: pathogenesis and prevention. Sternal osteomyelitis and mediastinitis caused by Pseudomonas cepacia developed in a patient undergoing coronary artery bypass two weeks after the operation. P. cepacia bacteremia from a contaminated pressure transducer had preceded and probably caused the chest infection. While other authors have suggested that postoperative sternal osteomyelitis and mediastinitis result from local wound contamination, this case suggests the importance of bacteremia as a cause of such gram-negative infections. Since patients undergoing open-heart operation are exposed to many sources of bacteremia, prevention of severe postoperative chest infections may depend in large part on careful preoperative evaluation of each patienc antibiotic regimens, and, as shown in this patient, on very thorough periodic review of equipment sterilization and intravascular monitoring practices."}
{"id": "PMID:1083720", "title": "A new approach to left heart decompression.", "content": "A simple, safe, indirect technique for decompression of the left heart is described. Pulmonary artery venting produces immediate and total decompression of the left heart through spontaneous retrograde pulmonary flow across the open mitral valve in the hypothermic (29 degrees C) electrically fibrillated heart. This allows free retrograde flow of left heart blood across the pulmonary valve into the right ventricle, where it is taken up by the usual caval cannulas. In 25 of 66 consecutive patients undergoing elective ventricular fibrillation and anoxic arrest, left heart venting was necessary as indicated by rising left heart pressures. Total cardiac decompression was immediately and completely achieved by this simple indirect technique. Direct left heart venting, with its associated risks and disadvantages, was never necessary, and we now consider it obsolete.", "contents": "A new approach to left heart decompression. A simple, safe, indirect technique for decompression of the left heart is described. Pulmonary artery venting produces immediate and total decompression of the left heart through spontaneous retrograde pulmonary flow across the open mitral valve in the hypothermic (29 degrees C) electrically fibrillated heart. This allows free retrograde flow of left heart blood across the pulmonary valve into the right ventricle, where it is taken up by the usual caval cannulas. In 25 of 66 consecutive patients undergoing elective ventricular fibrillation and anoxic arrest, left heart venting was necessary as indicated by rising left heart pressures. Total cardiac decompression was immediately and completely achieved by this simple indirect technique. Direct left heart venting, with its associated risks and disadvantages, was never necessary, and we now consider it obsolete."}
{"id": "PMID:1083721", "title": "A simple and effective left ventricular vent tube.", "content": "We have devised a simple, cheap, left ventricular vent that is relatively free from malfunction. It has further use in that it serves as a means of cooling the endocardium when hypothermic techniques are utilized.", "contents": "A simple and effective left ventricular vent tube. We have devised a simple, cheap, left ventricular vent that is relatively free from malfunction. It has further use in that it serves as a means of cooling the endocardium when hypothermic techniques are utilized."}
{"id": "PMID:1083722", "title": "Antibodies to components of extractable nuclear antigen. Clinical characteristics of patients.", "content": "Forty-four patients with antibodies to ribonuclease-sensitive extractable nuclear antigen (ENA), ribonuclease-resistant ENA, or both, are described. Most patients with antiribonucleoprotein (anti-RNP) antibodies have overlapping features of systemic lupus erythematosus (SLE), progressive systemic sclerosis (PSS), and polymyositis, and have a low incidence of nephritis. Most patients with antibody solely to ribonuclease-insensitive ENA have SLE; these patients with SLE are typical of the general SLE population, except that they demonstrate an increased incidence of Raynaud phenomenon. Furthermore, it is shown that antibody to ENA may occur in other rheumatic and nonrheumatic diseases, and that not all patients who have a clinical overlap of SLE and PSS that is suggestive of mixed connective tissue disease have anti-RNP antibody.", "contents": "Antibodies to components of extractable nuclear antigen. Clinical characteristics of patients. Forty-four patients with antibodies to ribonuclease-sensitive extractable nuclear antigen (ENA), ribonuclease-resistant ENA, or both, are described. Most patients with antiribonucleoprotein (anti-RNP) antibodies have overlapping features of systemic lupus erythematosus (SLE), progressive systemic sclerosis (PSS), and polymyositis, and have a low incidence of nephritis. Most patients with antibody solely to ribonuclease-insensitive ENA have SLE; these patients with SLE are typical of the general SLE population, except that they demonstrate an increased incidence of Raynaud phenomenon. Furthermore, it is shown that antibody to ENA may occur in other rheumatic and nonrheumatic diseases, and that not all patients who have a clinical overlap of SLE and PSS that is suggestive of mixed connective tissue disease have anti-RNP antibody."}
{"id": "PMID:1083724", "title": "Subcutaneous electrical stimulation (acupuncture) in the clinical practice of physical medicine.", "content": "There has been a rapid growth in both interest and concern over the value of acupuncture in this country during the last three years. Initially it was regarded with doubt, then with overwhelming interest, but only recently have any research data appeared as to its true value and applicability to western medicine. An acupuncture research project was initiated in November 1972 by the author to assess the immediate and long-range benefits of acupuncture in a group of 179 patients with various neuromuscular-skeletal pain and mobility problems. A total of 1,112 treatments have been administered. A follow-up survey of these patients was completed in October 1974 with 126 (70%) of the patients responding to a subjective questionnaire. In 49% of the patients the initial response to acupuncture treatments was some reduction of symptoms and in a significant percentage there was persistence of beneficial response for greater than three months.", "contents": "Subcutaneous electrical stimulation (acupuncture) in the clinical practice of physical medicine. There has been a rapid growth in both interest and concern over the value of acupuncture in this country during the last three years. Initially it was regarded with doubt, then with overwhelming interest, but only recently have any research data appeared as to its true value and applicability to western medicine. An acupuncture research project was initiated in November 1972 by the author to assess the immediate and long-range benefits of acupuncture in a group of 179 patients with various neuromuscular-skeletal pain and mobility problems. A total of 1,112 treatments have been administered. A follow-up survey of these patients was completed in October 1974 with 126 (70%) of the patients responding to a subjective questionnaire. In 49% of the patients the initial response to acupuncture treatments was some reduction of symptoms and in a significant percentage there was persistence of beneficial response for greater than three months."}
{"id": "PMID:1083725", "title": "Acupuncture effect on neurosensory deafness.", "content": "Bilateral neurosensory deafness offers a unique opportunity to study the effects of acupuncture objectively. By treating only one ear, and using audiometric evaluation techniques instead of subjective evaluations of hearing, an analysis of any achieved improvement seemed feasible. Only patients with well documented bilateral nerve deafness were accepted into the program. A baseline audiogram with speech discrimination and puretone air and bone conduction thresholds was obtained prior to treatment. Thirty-eight patients underwent ten consecutive acupuncture treatments on one ear only, with the other ear serving as a control. After the course of acupuncture, the audiometrics were repeated by the same audiologist. There was no significant increase in hearing after acupuncture therapy.", "contents": "Acupuncture effect on neurosensory deafness. Bilateral neurosensory deafness offers a unique opportunity to study the effects of acupuncture objectively. By treating only one ear, and using audiometric evaluation techniques instead of subjective evaluations of hearing, an analysis of any achieved improvement seemed feasible. Only patients with well documented bilateral nerve deafness were accepted into the program. A baseline audiogram with speech discrimination and puretone air and bone conduction thresholds was obtained prior to treatment. Thirty-eight patients underwent ten consecutive acupuncture treatments on one ear only, with the other ear serving as a control. After the course of acupuncture, the audiometrics were repeated by the same audiologist. There was no significant increase in hearing after acupuncture therapy."}
{"id": "PMID:1083726", "title": "Chronic consumption coagulopathy accompanying abdominal aortic aneurysm.", "content": "Each of two patients harboring a stable abdominal aortic aneurysm manifested severe recurrent bleeding consequent to chronic consumption coagulopathy (CCC). Both underwent successful aneurysmectomy, but in only one patient did bleeding cease and depressed clotting factors return to normal activity. In the other patient, subsequent observations suggested that his coagulopathy actually resulted from occult pancreatic carcinoma. We propose here criteria for establishing stable aneurysm as the cause of CCC and demonstrate the efficacy of heparin in reversing the coagulation defect prior to surgical intervention. These cases also illustrate that the discovery of CCC accompanying stable aneurysm may signal the presence of another underlying disorder.", "contents": "Chronic consumption coagulopathy accompanying abdominal aortic aneurysm. Each of two patients harboring a stable abdominal aortic aneurysm manifested severe recurrent bleeding consequent to chronic consumption coagulopathy (CCC). Both underwent successful aneurysmectomy, but in only one patient did bleeding cease and depressed clotting factors return to normal activity. In the other patient, subsequent observations suggested that his coagulopathy actually resulted from occult pancreatic carcinoma. We propose here criteria for establishing stable aneurysm as the cause of CCC and demonstrate the efficacy of heparin in reversing the coagulation defect prior to surgical intervention. These cases also illustrate that the discovery of CCC accompanying stable aneurysm may signal the presence of another underlying disorder."}
{"id": "PMID:1083727", "title": "Recurrent variceal hemorrhage following successful Warren shunt.", "content": "Recurrent variceal hemorrhages are demonstrated following a technically successful Warren shunt. Serial barium esophagrams show the progressive enlargement of distal esophageal varices. Results of celiac arteriograms and direct examination confirmed the patency of the distal splenorenal shunt. Hemorrhage from varices was shown by fiberoptic esophagoscopy; end-to-side portacaval anastomosis reduced portal pressure and stopped the bleeding.", "contents": "Recurrent variceal hemorrhage following successful Warren shunt. Recurrent variceal hemorrhages are demonstrated following a technically successful Warren shunt. Serial barium esophagrams show the progressive enlargement of distal esophageal varices. Results of celiac arteriograms and direct examination confirmed the patency of the distal splenorenal shunt. Hemorrhage from varices was shown by fiberoptic esophagoscopy; end-to-side portacaval anastomosis reduced portal pressure and stopped the bleeding."}
{"id": "PMID:1083728", "title": "Transhepatic variceal sclerotherapy.", "content": "We attempted to control variceal hemorrhage, using transhepatic variceal sclerotherapy of a coronary varix. The procedure transiently controlled hemorrhage, but bleeding resumed from short gastric varices hours later.", "contents": "Transhepatic variceal sclerotherapy. We attempted to control variceal hemorrhage, using transhepatic variceal sclerotherapy of a coronary varix. The procedure transiently controlled hemorrhage, but bleeding resumed from short gastric varices hours later."}
{"id": "PMID:1083729", "title": "Coagulation factor VIII. Localization in the aqueous outflow pathways.", "content": "Antihemophilic factor A-von Willebrand factor (AHF-vWF) is a protein involved in the first phase of blood coagulation and in the mechanism of platelet adhesion. The localization of this protein has been studied in the anterior segment of fetal and adult human eye by a direct immunofluorescence method. It was found that AHF-vWF is present in the wall of aqueous outflow pathways but not in that of Schlemm canal. As it has fibrinolytic activity in the endothelium of Schlemm canal, this structure can be expected to be especially well protected against fibrin deposition and occlusion by platelets and thrombi. The ciliary processes of the adult eye had no AHF-vWF activity. Instead, fluorescein isothiocyanate-fluorescence could be seen in the vessel wall of the ciliary processes of the fetal eye. This difference may be connected with the function of aqueous secretion.", "contents": "Coagulation factor VIII. Localization in the aqueous outflow pathways. Antihemophilic factor A-von Willebrand factor (AHF-vWF) is a protein involved in the first phase of blood coagulation and in the mechanism of platelet adhesion. The localization of this protein has been studied in the anterior segment of fetal and adult human eye by a direct immunofluorescence method. It was found that AHF-vWF is present in the wall of aqueous outflow pathways but not in that of Schlemm canal. As it has fibrinolytic activity in the endothelium of Schlemm canal, this structure can be expected to be especially well protected against fibrin deposition and occlusion by platelets and thrombi. The ciliary processes of the adult eye had no AHF-vWF activity. Instead, fluorescein isothiocyanate-fluorescence could be seen in the vessel wall of the ciliary processes of the fetal eye. This difference may be connected with the function of aqueous secretion."}
{"id": "PMID:1083730", "title": "T-cell rosette test in squamous cell carcinoma of the head and neck.", "content": "The percentage of total T-lymphocytes was measured in 38 patients with histologically diagnosed squamous cell carcinoma of the head and neck. This measure of cellular immunity, which appears to be important in tumor-cell destruction, is demonstrably lowered in advanced stages of this disease. With the use of this test, the effect of a combined chemoimmunotherapeutic regimen consisting of methotrexate sodium, isoniazid, and BCG vaccine was sutdied. In this small series, no decrease of the T-cell count following treatment by methotrexate was demonstrated, except when a high dosage followed by leucovorin calcium was used. The BCG vaccine appeared to enhance T-cell counts, and one patient who was treated with cryosurgery had a dramatic increase in circulating T-lymphocytes. The T-cell test may be of clinical importance in the development of treatment sequences that provide maximum reduction of tumor cell mass while preserving host T cells.", "contents": "T-cell rosette test in squamous cell carcinoma of the head and neck. The percentage of total T-lymphocytes was measured in 38 patients with histologically diagnosed squamous cell carcinoma of the head and neck. This measure of cellular immunity, which appears to be important in tumor-cell destruction, is demonstrably lowered in advanced stages of this disease. With the use of this test, the effect of a combined chemoimmunotherapeutic regimen consisting of methotrexate sodium, isoniazid, and BCG vaccine was sutdied. In this small series, no decrease of the T-cell count following treatment by methotrexate was demonstrated, except when a high dosage followed by leucovorin calcium was used. The BCG vaccine appeared to enhance T-cell counts, and one patient who was treated with cryosurgery had a dramatic increase in circulating T-lymphocytes. The T-cell test may be of clinical importance in the development of treatment sequences that provide maximum reduction of tumor cell mass while preserving host T cells."}
{"id": "PMID:1083731", "title": "[Postpartum hemorrhage syndrome caused by factor VIII inhibitor in the blood].", "content": "The appearance of Factor VIII inhibitor in women after childbirth is a very rare occurence. However the hemorrhagic syndrome which results from such condition can be very serious and difficult to treat. In this paper a case of young women is described who developed after childbirth a severe bleeding disorder. Clinical feature was very similar to those seen in haemophilia. The patient was investigated in the local hospital but the diagnosis was not made. The bleeding disorder lasted with various extension for six months and patient finally developed a severe sublingual and laringeal bleeding. She was transfered to the Clinic \"B\" of Internal Medicine in a very serious condition. On examination there was marked prolongation of the clotting time and no detectable Factor VIII. On the basis of these findings and the clinical expression of bleeding disorders, the tentative diagnosis of Factor VIII inhibitor was made. The treatment was not possible to institute because the patient expired within hours. Autopsy showed that bleeding into deep laringeal tissue was the sole cause of her death. Additional examination of the of the patient's plasma specimen using the antibody assay (Biggs-Bidwell-1959) showed the presence of the antibody to the Factor Viii which were active in 1:100 dilution.", "contents": "[Postpartum hemorrhage syndrome caused by factor VIII inhibitor in the blood]. The appearance of Factor VIII inhibitor in women after childbirth is a very rare occurence. However the hemorrhagic syndrome which results from such condition can be very serious and difficult to treat. In this paper a case of young women is described who developed after childbirth a severe bleeding disorder. Clinical feature was very similar to those seen in haemophilia. The patient was investigated in the local hospital but the diagnosis was not made. The bleeding disorder lasted with various extension for six months and patient finally developed a severe sublingual and laringeal bleeding. She was transfered to the Clinic \"B\" of Internal Medicine in a very serious condition. On examination there was marked prolongation of the clotting time and no detectable Factor VIII. On the basis of these findings and the clinical expression of bleeding disorders, the tentative diagnosis of Factor VIII inhibitor was made. The treatment was not possible to institute because the patient expired within hours. Autopsy showed that bleeding into deep laringeal tissue was the sole cause of her death. Additional examination of the of the patient's plasma specimen using the antibody assay (Biggs-Bidwell-1959) showed the presence of the antibody to the Factor Viii which were active in 1:100 dilution."}
{"id": "PMID:1083737", "title": "Anti-tumour and anti-metastatic activity of 3-(P-Chlorophenyl)-2,3-Dihydro-3-Hydroxythiazolo (3,2-A)-Benzimidazole-2-acetic acid (WY-13,876).", "content": "Extensive investigation of 3-(p-chlorophenyl)-2,3-dihydro-3-hydroxythiazolo(3,2-alpha)-benzimidazole-2-acetic acid (Wy-13,876) in BDF1 mice implanted with Lewis lung tumour has shown that it is an effective anti-tumour and anti-metastatic agent. In vitro examination using HEp-2 human epidermal tumour cells has indicated that Wy-13,876 is not cytotoxic. When mice implanted with Lewis lung tumour and treated with Wy-13,876 are also injected with anti-thymocyte serum, an increase in lung metastases is observed suggesting that thymocyte activity is involved in the drug's mechanism of action. An increase in peripheral T lymphocytes observed in rats 18 h after a single oral dose of Wy-13,876 further supports this possibility. When Wy-13,876 is given to tumour -bearing mice in combination with low, ineffective doses of 5-fluorouracil or cyclophosphamide, further reduction of primary tumour growth is observed.", "contents": "Anti-tumour and anti-metastatic activity of 3-(P-Chlorophenyl)-2,3-Dihydro-3-Hydroxythiazolo (3,2-A)-Benzimidazole-2-acetic acid (WY-13,876). Extensive investigation of 3-(p-chlorophenyl)-2,3-dihydro-3-hydroxythiazolo(3,2-alpha)-benzimidazole-2-acetic acid (Wy-13,876) in BDF1 mice implanted with Lewis lung tumour has shown that it is an effective anti-tumour and anti-metastatic agent. In vitro examination using HEp-2 human epidermal tumour cells has indicated that Wy-13,876 is not cytotoxic. When mice implanted with Lewis lung tumour and treated with Wy-13,876 are also injected with anti-thymocyte serum, an increase in lung metastases is observed suggesting that thymocyte activity is involved in the drug's mechanism of action. An increase in peripheral T lymphocytes observed in rats 18 h after a single oral dose of Wy-13,876 further supports this possibility. When Wy-13,876 is given to tumour -bearing mice in combination with low, ineffective doses of 5-fluorouracil or cyclophosphamide, further reduction of primary tumour growth is observed."}
{"id": "PMID:1083738", "title": "Estimation of plasma esterolytic activity and its in vitro inhibition by proteinase inhibitors during acute pancreatitis in the human.", "content": "The plasma esterolytic activity was measured using benzyol arginine ethyl ester (BAEe) in the peripheral venous blood of patients with acute pancreatitis, normal healthy volunteers and a contrast group of patients with acute intrabdominal inflammations other than acute pancreatitis. The plasma esterolytic activity was significantly elevated in the pancreatitis group. This activity was maximal during the first 48 hours of the illness and remained elevated for a further 8 days thereafter. Aprotinin in a dose of 2000 K.I. u/0-3 ml plasma did not completely inhibit this esterolytic activity, although it resulted in a more substantial inhibition than either ovomucoid or soy bean inhibitor. It is concluded that pancreatic enzymes are released into the circulation during acute pancreatitis and that Aprotinin does not completely inhibit this proteolytic activity. This polyvalent proteinase inhibitor should therefore be administered in much higher dosage than that used hitherto in  acute pancreatitis. The plasma esterolytic activity seems to be of diagnostic value in acute  pancreatitis.", "contents": "Estimation of plasma esterolytic activity and its in vitro inhibition by proteinase inhibitors during acute pancreatitis in the human. The plasma esterolytic activity was measured using benzyol arginine ethyl ester (BAEe) in the peripheral venous blood of patients with acute pancreatitis, normal healthy volunteers and a contrast group of patients with acute intrabdominal inflammations other than acute pancreatitis. The plasma esterolytic activity was significantly elevated in the pancreatitis group. This activity was maximal during the first 48 hours of the illness and remained elevated for a further 8 days thereafter. Aprotinin in a dose of 2000 K.I. u/0-3 ml plasma did not completely inhibit this esterolytic activity, although it resulted in a more substantial inhibition than either ovomucoid or soy bean inhibitor. It is concluded that pancreatic enzymes are released into the circulation during acute pancreatitis and that Aprotinin does not completely inhibit this proteolytic activity. This polyvalent proteinase inhibitor should therefore be administered in much higher dosage than that used hitherto in  acute pancreatitis. The plasma esterolytic activity seems to be of diagnostic value in acute  pancreatitis."}
{"id": "PMID:1083739", "title": "Immunological aspects of psoriasis. I. Immunoglobulins and anti-IgG factors.", "content": "Sixty patients with psoriasis have been studied for quantitative estimation of serum immunoglobulin A, G and M (60 cases), immunoglobulin E (43 cases), salivary immunoglobulin A (28 cases) and anti-IgG factors in the serum (40 cases). All the data have been statistically compared to a large control group (300 subjects). The results show an increase of the serum IgG and IgA means, an increase of salivary IgA which is correlated with the serum IgA, an increase of IgE levels in some patients and the presence of anti-IgG activity in the serum of 45% of patients with psoriasis. All these findings are statistically significant when compared to controls. These data could be in favour of auto-immune processes in psoriasis.", "contents": "Immunological aspects of psoriasis. I. Immunoglobulins and anti-IgG factors. Sixty patients with psoriasis have been studied for quantitative estimation of serum immunoglobulin A, G and M (60 cases), immunoglobulin E (43 cases), salivary immunoglobulin A (28 cases) and anti-IgG factors in the serum (40 cases). All the data have been statistically compared to a large control group (300 subjects). The results show an increase of the serum IgG and IgA means, an increase of salivary IgA which is correlated with the serum IgA, an increase of IgE levels in some patients and the presence of anti-IgG activity in the serum of 45% of patients with psoriasis. All these findings are statistically significant when compared to controls. These data could be in favour of auto-immune processes in psoriasis."}
{"id": "PMID:1083740", "title": "Identification of subpopulations of lymphocytes and macrophages in the infiltrate of lichen planus lesions of skin and oral mucosa.", "content": "The mononuclear cells of the infiltrate in lichen planus lesions of skin and oral mucosa have been identified by detection of their membrane receptors in frozen-tissue sections. By this method, the infiltrate would appear to consist of predominantly T lymphocytes, some macrophages and only a few B cells. The pathogenesis of lichen planus is discussed in the light of these findings.", "contents": "Identification of subpopulations of lymphocytes and macrophages in the infiltrate of lichen planus lesions of skin and oral mucosa. The mononuclear cells of the infiltrate in lichen planus lesions of skin and oral mucosa have been identified by detection of their membrane receptors in frozen-tissue sections. By this method, the infiltrate would appear to consist of predominantly T lymphocytes, some macrophages and only a few B cells. The pathogenesis of lichen planus is discussed in the light of these findings."}
{"id": "PMID:1083741", "title": "The effect of transfer factor on neutrophil function in chronic mucocutaneous candidiasis.", "content": "Chronic mucocutaneous candidiasis with hypoparathyroidism in a 6-year-old-boy is described. In addition to defects of in vivo and in vitro correlates of delayed-type hypersensitivity to Candida albicans the child also had abnormalities of neutrophil function in terms of their capacity to respond by chemotaxis to a known attractant and to kill suspensions of C. albicans. Dialysable transfer factor was given on six occasions at intervals of between 26 and 45 days. Neutrophil chemotaxis (optimal conditions) was restored following each of the six injections, neutrophil chemotaxis (sub-optimal conditions) following five of the six injections and candidicidal capacity following four of the six injections. The effects of transfer factor were transient requiring repeated injections. The Candida delayed-type hypersensitivity skin test was restored to normal but lymphocyte transformation to Candida extract was not consistently positive following treatment. There was a slight clinical improvement following therapy. These abnormalities of neutrophil and lymphocyte function point to the complexity of chronic mucocutaneous candidiasis. The improvement in neutrophil chemotaxis and candidicidal capacity following treatment suggests that transfer factor may be a heterogeneous group of molecules, some of which affect granulocytes and restore defects in their function.", "contents": "The effect of transfer factor on neutrophil function in chronic mucocutaneous candidiasis. Chronic mucocutaneous candidiasis with hypoparathyroidism in a 6-year-old-boy is described. In addition to defects of in vivo and in vitro correlates of delayed-type hypersensitivity to Candida albicans the child also had abnormalities of neutrophil function in terms of their capacity to respond by chemotaxis to a known attractant and to kill suspensions of C. albicans. Dialysable transfer factor was given on six occasions at intervals of between 26 and 45 days. Neutrophil chemotaxis (optimal conditions) was restored following each of the six injections, neutrophil chemotaxis (sub-optimal conditions) following five of the six injections and candidicidal capacity following four of the six injections. The effects of transfer factor were transient requiring repeated injections. The Candida delayed-type hypersensitivity skin test was restored to normal but lymphocyte transformation to Candida extract was not consistently positive following treatment. There was a slight clinical improvement following therapy. These abnormalities of neutrophil and lymphocyte function point to the complexity of chronic mucocutaneous candidiasis. The improvement in neutrophil chemotaxis and candidicidal capacity following treatment suggests that transfer factor may be a heterogeneous group of molecules, some of which affect granulocytes and restore defects in their function."}
{"id": "PMID:1083742", "title": "Mouse red-cell rosettes in B-lymphoproliferative disorders.", "content": "A high proportion of peripheral-blood lymphocytes formed spontaneous rosettes with mouse red cells in 22 out of 23 cases of chronic lymphocytic leukaemia (CLL); the proportion was significantly higher than in 19 cases of other B-lymphoproliferative disorders (non-CLL group) and in 19 normal controls. Intermediate findings were obtained in 10 cases of \"hairy\" cell leukaemia. Blast cells from various types of acute leukaemia did not bind mouse red cells. Pre-treatment of the lymphocytes with neuraminidase led to a significant increase in the proportion of rosettes in CLL only. This test may prove useful in distinguishing CLL from other B-lymphoproliferative disorders, particularly prolymphocytic leukaemia.", "contents": "Mouse red-cell rosettes in B-lymphoproliferative disorders. A high proportion of peripheral-blood lymphocytes formed spontaneous rosettes with mouse red cells in 22 out of 23 cases of chronic lymphocytic leukaemia (CLL); the proportion was significantly higher than in 19 cases of other B-lymphoproliferative disorders (non-CLL group) and in 19 normal controls. Intermediate findings were obtained in 10 cases of \"hairy\" cell leukaemia. Blast cells from various types of acute leukaemia did not bind mouse red cells. Pre-treatment of the lymphocytes with neuraminidase led to a significant increase in the proportion of rosettes in CLL only. This test may prove useful in distinguishing CLL from other B-lymphoproliferative disorders, particularly prolymphocytic leukaemia."}
{"id": "PMID:1083743", "title": "Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease.", "content": "A solid phase non-competitive immunoradiometric assay (IRMA) has been developed which allows measurement of factor VIII related antigen (VIIIR:AG) levels in normal plasma as low as 2.5 x 10(-4) U/ml. The assay is based on the extraction of VIIIR:AG from test plasma by means of polystrene tubes coated with a specific unlabelled anti-VIIIR:AG rabbit antiserum and subsequent labelling of the extracted antigen with 125I-labelled anti-VIIIR:AG rabbit IgG.", "contents": "Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease. A solid phase non-competitive immunoradiometric assay (IRMA) has been developed which allows measurement of factor VIII related antigen (VIIIR:AG) levels in normal plasma as low as 2.5 x 10(-4) U/ml. The assay is based on the extraction of VIIIR:AG from test plasma by means of polystrene tubes coated with a specific unlabelled anti-VIIIR:AG rabbit antiserum and subsequent labelling of the extracted antigen with 125I-labelled anti-VIIIR:AG rabbit IgG."}
{"id": "PMID:1083744", "title": "Recurrent erosion of the cornea.", "content": "Altogether, 80 patients aged between 24 and 73 years with recurrent erosion of the cornea have been studied and compared with a control group of 200. The patients' erosions were divisible into macroform and microform types. The macroform occurred in 10%, the microform in 56%, and both types in the same patients in 31%. The macroform was more commonly related to trauma than the microform. However, many (40%) were spontaneous in origin. The most common cause of the initial trauma was a finger nail. The recurrences occurred at around the time of waking, either just before or just after. Difficulty in opening the eye occurred in 10%. There was little evidence of precipitating factors, but eye rubbing was admitted by 10% and barbiturates were implicated in 3%. The corneae were examined in the healed state, when a high incidence (59%) were found to have superficial corneal dystrophies of the fingerprint lines, bleb, and Bietti's lacunar (map-like) types. These are considered individually, particular attention being paid to the distinction between the various types of line resembling the fingerprint line. Epithelial microcysts were also a common finding (59%) and were sometimes of the Cogan type. In only 11% of patients were there no corneal signs in the healed state. The need for careful examination of the cornea by retroillumination, using both the iris and the fundus, is stressed. The control group, in contrast, showed a very low incidence of dystrophies and cysts. Treatment was given initially with either drops or ointment and no differences in healing were found. Debridement was performed in 12 eyes as an initial treatment and also in four eyes which were not healing on medical treatment. Debridement assisted healing, but did not prevent recurrence. One eye was treated with debridement and scarification and seven with carbolization. These procedures appeared to reduce the recurrence rate. Sodium chloride ointment 5% was found useful as a prophylactic taken at bedtime, and the recurrence rate increased when it was withdrawn.", "contents": "Recurrent erosion of the cornea. Altogether, 80 patients aged between 24 and 73 years with recurrent erosion of the cornea have been studied and compared with a control group of 200. The patients' erosions were divisible into macroform and microform types. The macroform occurred in 10%, the microform in 56%, and both types in the same patients in 31%. The macroform was more commonly related to trauma than the microform. However, many (40%) were spontaneous in origin. The most common cause of the initial trauma was a finger nail. The recurrences occurred at around the time of waking, either just before or just after. Difficulty in opening the eye occurred in 10%. There was little evidence of precipitating factors, but eye rubbing was admitted by 10% and barbiturates were implicated in 3%. The corneae were examined in the healed state, when a high incidence (59%) were found to have superficial corneal dystrophies of the fingerprint lines, bleb, and Bietti's lacunar (map-like) types. These are considered individually, particular attention being paid to the distinction between the various types of line resembling the fingerprint line. Epithelial microcysts were also a common finding (59%) and were sometimes of the Cogan type. In only 11% of patients were there no corneal signs in the healed state. The need for careful examination of the cornea by retroillumination, using both the iris and the fundus, is stressed. The control group, in contrast, showed a very low incidence of dystrophies and cysts. Treatment was given initially with either drops or ointment and no differences in healing were found. Debridement was performed in 12 eyes as an initial treatment and also in four eyes which were not healing on medical treatment. Debridement assisted healing, but did not prevent recurrence. One eye was treated with debridement and scarification and seven with carbolization. These procedures appeared to reduce the recurrence rate. Sodium chloride ointment 5% was found useful as a prophylactic taken at bedtime, and the recurrence rate increased when it was withdrawn."}
{"id": "PMID:1083745", "title": "The inhibition of human leucocyte elastase and chymotrypsin-like protease by elastatinal and chymostatin.", "content": "The ability of elastatinal and chymostatin, protease inhibitors of microbial origin, to inhibit human leucocyte proteases (EC 3.4.-) was studied. Elastatinal and chymostatin are capable of inhibiting the pancreatic enzymes elastase and chymotrypsin, respectively. It was found in these studies, with synthetic substrates, that elastatinal is a much weaker inhibitor of human leucocyte elastase than it is of porcine pancreatic elastase. Elastatinal caused no inhibition of the activity of human leucocyte chymotrypsin-like protease. Chymostatin was found to be a powerful inhibitor of human leucocyte chymotrypsin-like protease. Its affinity to the leucocyte protease was higher than its affinity to bovine pancreatic alpha-chymotrypsin. Chymsotatin had a weak inhibitory effect on the activity of human leucocyte elastase. Studies were also carried out on the ability of chymostatin to inhibit the release of 35SO2-4 from rabbit articular cartilage by human leucocyte chymotrypsin-like protease. Preincubation of the chymostatin with the protease before the latter was added to the 35SO2-4 -labeled cartilage caused inhibition of proteolysis as measured by 35SO2-4 release. Preincubation of chymostatin with 35SO2-4 -labeled cartilage prior to addition of the human chymotrypsin-like protease to the tissue also inhibited 35SO2-4 release. However, in the case of preincubation of cartilage with alpha1 -antitrypsin there was no such inhibition. It therefore appeared that chymostatin, unlike alpha1 -antitrypsin, was capable of penetrating the cartilage matrix and exerting its inhibitory effect upon the human leucocyte chymotrypsin-like protease that was subsequently added to the tissue.", "contents": "The inhibition of human leucocyte elastase and chymotrypsin-like protease by elastatinal and chymostatin. The ability of elastatinal and chymostatin, protease inhibitors of microbial origin, to inhibit human leucocyte proteases (EC 3.4.-) was studied. Elastatinal and chymostatin are capable of inhibiting the pancreatic enzymes elastase and chymotrypsin, respectively. It was found in these studies, with synthetic substrates, that elastatinal is a much weaker inhibitor of human leucocyte elastase than it is of porcine pancreatic elastase. Elastatinal caused no inhibition of the activity of human leucocyte chymotrypsin-like protease. Chymostatin was found to be a powerful inhibitor of human leucocyte chymotrypsin-like protease. Its affinity to the leucocyte protease was higher than its affinity to bovine pancreatic alpha-chymotrypsin. Chymsotatin had a weak inhibitory effect on the activity of human leucocyte elastase. Studies were also carried out on the ability of chymostatin to inhibit the release of 35SO2-4 from rabbit articular cartilage by human leucocyte chymotrypsin-like protease. Preincubation of the chymostatin with the protease before the latter was added to the 35SO2-4 -labeled cartilage caused inhibition of proteolysis as measured by 35SO2-4 release. Preincubation of chymostatin with 35SO2-4 -labeled cartilage prior to addition of the human chymotrypsin-like protease to the tissue also inhibited 35SO2-4 release. However, in the case of preincubation of cartilage with alpha1 -antitrypsin there was no such inhibition. It therefore appeared that chymostatin, unlike alpha1 -antitrypsin, was capable of penetrating the cartilage matrix and exerting its inhibitory effect upon the human leucocyte chymotrypsin-like protease that was subsequently added to the tissue."}
{"id": "PMID:1083749", "title": "T-cell qualities of lymphocytes and/or leukemic cells from peripheral blood in acute myeloid and lymphatic leukemia before treatment and at relapse.", "content": "PHA stimulation of peripheral blood lymphocytes and/or leukemic cells has been studied in 65 patients with acute leukemia before any prior treatment. There were 38 patients with A.L.L. and 27 with A.M.L. In relapsing A.L.L. 15 observations were made before reinduction therapy. In about 40% of all the cultures PHA stimulation was done simultaneously with PWM-stimulation. Before any treatment normal lymphocytes showed a normal response, being however somewhat diminished at relapse. Most of the cases of A.M.L. had so many leukemic cells in peripheral blood that no reliable measure of stimulation of the few lymphocytes left was possible. In A.L.L. this was however possible in about 30% of all the cases studied. In cultures of the predominantly leukemic cells, lymphoblasts showed a positive response in a minority of cases studied. If the peripheral WBC-count was high, a response was usually absent. In some of the cases of A.M.L., the leukemic cells reacted to PHA and PWM. Unexpected but very remarkable is our finding that in the control cultures, without added mitogen, the incorporation of labelled 3H-thymidine was usually very high in A.M.L. contrasting with A.L.L. leukemic cells who incorporated very little of the label. These striking differences were observed both in 72 and 144 hours cultures and were not caused by differences in viability between these two leukemic cell-types. The capacity of A.L.L.-leukemic cells to form E (\"T\") and EAC (\"B\") rosettes was studied in 20 cases. The percentages of rosette forming lymphoblasts differed widely from patient to patient. It is concluded that in A.L.L. leukemic cells are in a minority immuno-competent cells and it is suggested that the lymphoblast is an immature cell, arrested in the maturation towards a T-lymphocyte, the degree of maturity differing from patient to patient. Furthermore the striking difference in spontaneous labelling of myeloid versus lymphoblastic leukemic cells seems to be helpful in the differential diagnosis and may in part explain the well known differences in prognosis and behaviour towards cytostatics between these main leukemia types.", "contents": "T-cell qualities of lymphocytes and/or leukemic cells from peripheral blood in acute myeloid and lymphatic leukemia before treatment and at relapse. PHA stimulation of peripheral blood lymphocytes and/or leukemic cells has been studied in 65 patients with acute leukemia before any prior treatment. There were 38 patients with A.L.L. and 27 with A.M.L. In relapsing A.L.L. 15 observations were made before reinduction therapy. In about 40% of all the cultures PHA stimulation was done simultaneously with PWM-stimulation. Before any treatment normal lymphocytes showed a normal response, being however somewhat diminished at relapse. Most of the cases of A.M.L. had so many leukemic cells in peripheral blood that no reliable measure of stimulation of the few lymphocytes left was possible. In A.L.L. this was however possible in about 30% of all the cases studied. In cultures of the predominantly leukemic cells, lymphoblasts showed a positive response in a minority of cases studied. If the peripheral WBC-count was high, a response was usually absent. In some of the cases of A.M.L., the leukemic cells reacted to PHA and PWM. Unexpected but very remarkable is our finding that in the control cultures, without added mitogen, the incorporation of labelled 3H-thymidine was usually very high in A.M.L. contrasting with A.L.L. leukemic cells who incorporated very little of the label. These striking differences were observed both in 72 and 144 hours cultures and were not caused by differences in viability between these two leukemic cell-types. The capacity of A.L.L.-leukemic cells to form E (\"T\") and EAC (\"B\") rosettes was studied in 20 cases. The percentages of rosette forming lymphoblasts differed widely from patient to patient. It is concluded that in A.L.L. leukemic cells are in a minority immuno-competent cells and it is suggested that the lymphoblast is an immature cell, arrested in the maturation towards a T-lymphocyte, the degree of maturity differing from patient to patient. Furthermore the striking difference in spontaneous labelling of myeloid versus lymphoblastic leukemic cells seems to be helpful in the differential diagnosis and may in part explain the well known differences in prognosis and behaviour towards cytostatics between these main leukemia types."}
{"id": "PMID:1083750", "title": "[Choleragenic intoxication in hydrobionts].", "content": "Experiments were conducted on Rana temporaria in which cholerigenic intoxication was induced. Administration of high doses of choleragen caused in the epithelial cells of the small intestine of frogs structural-functional changes analogous to those observed in suckling rabbits used as a classic model for studying the mechanisms of cholerigenic intoxication.", "contents": "[Choleragenic intoxication in hydrobionts]. Experiments were conducted on Rana temporaria in which cholerigenic intoxication was induced. Administration of high doses of choleragen caused in the epithelial cells of the small intestine of frogs structural-functional changes analogous to those observed in suckling rabbits used as a classic model for studying the mechanisms of cholerigenic intoxication."}
{"id": "PMID:1083751", "title": "[T-lymphocyte migration into the bone marrow in the initial period of the stress reaction].", "content": "Six to nine hours after the beginning of stress-reaction caused by a 6-hour immobilization there appeared, in the bone marrow of CBA mice, cells with the antigenic (theta-antigen) and functional (cells-helpers) characteristics of T-lymphocytes. Migration of T-lymphocytes into the bone marrow was regarded as the mechanism of increase in nonspecific resistance of the organism.", "contents": "[T-lymphocyte migration into the bone marrow in the initial period of the stress reaction]. Six to nine hours after the beginning of stress-reaction caused by a 6-hour immobilization there appeared, in the bone marrow of CBA mice, cells with the antigenic (theta-antigen) and functional (cells-helpers) characteristics of T-lymphocytes. Migration of T-lymphocytes into the bone marrow was regarded as the mechanism of increase in nonspecific resistance of the organism."}
{"id": "PMID:1083747", "title": "[Temperature dependence of the kinetics of dark reduction of bacteriochlorophyll P870, oxidized by impulse laser and continuous light in photosynthetic reaction center preparations from Rhodosuedomonas spheroides strain 1760-1].", "content": "A comparative study was carried out of temperature dependence of kinetics of dark reduction of bacteriochlorophyll P870 oxidized both by pulsed laser and continuous actinic light in preparations of photosynthetic reaction centres-bacteriochlorophyll-protein complexes from Rhodopseudomonas spheroides, strain 1760-1. Half-time of the recombination of primary products--P870+ and reduced primary electron acceptor A1, which decreases with temperature lowering from 180-240 ms at 120K, is determined. Values of the rate constant of electron transfer from A1 to secondary acceptors at 29,K (2.7.10-1s-1) and the activation energy of this reaction in the range of 298-255K which is 11.8 kcal/mole are calculated.", "contents": "[Temperature dependence of the kinetics of dark reduction of bacteriochlorophyll P870, oxidized by impulse laser and continuous light in photosynthetic reaction center preparations from Rhodosuedomonas spheroides strain 1760-1]. A comparative study was carried out of temperature dependence of kinetics of dark reduction of bacteriochlorophyll P870 oxidized both by pulsed laser and continuous actinic light in preparations of photosynthetic reaction centres-bacteriochlorophyll-protein complexes from Rhodopseudomonas spheroides, strain 1760-1. Half-time of the recombination of primary products--P870+ and reduced primary electron acceptor A1, which decreases with temperature lowering from 180-240 ms at 120K, is determined. Values of the rate constant of electron transfer from A1 to secondary acceptors at 29,K (2.7.10-1s-1) and the activation energy of this reaction in the range of 298-255K which is 11.8 kcal/mole are calculated."}
{"id": "PMID:1083756", "title": "Emergency arteriography in acute gastrointestinal bleeding.", "content": "Emergency arteriography was carried out on 35 patients with acute gastrointestinal bleeding, in 31 of them within two hours of active bleeding (a haematemisis; a diagnostic change in central venous pressure, pulse rate, or blood pressure; or gastric aspiration of fresh blood). A definite site of bleeding was identified in 27 patients (77%)-this being a small-intestinal vascular abnormality in three--and a probable site in three. Confirmation of the bleeding site was obtained in 20 out of 23 patients treated surgically. An intra-arterial vasoconstrictor infusion was given as a temporary measure before surgery in seven patients, only one of whom showed active bleeding at operation. An intra-arterial vasoconstrictor infusion was tried as definitive treatment in an additional 10 patients, but in four out of seven with a chronic ulcer bleeding recurred after 5-68 hours and was therefore treated surgically. We recommend the diagnostic use of arteriography in patients with reliable evidence of active bleeding if its site cannot be determined by endoscopy. We do not recommend its therapeutic use in those with a chronic ulcer, except to facilitate resuscitation before surgery; further studies are needed to define its role in those with an acute lesion.", "contents": "Emergency arteriography in acute gastrointestinal bleeding. Emergency arteriography was carried out on 35 patients with acute gastrointestinal bleeding, in 31 of them within two hours of active bleeding (a haematemisis; a diagnostic change in central venous pressure, pulse rate, or blood pressure; or gastric aspiration of fresh blood). A definite site of bleeding was identified in 27 patients (77%)-this being a small-intestinal vascular abnormality in three--and a probable site in three. Confirmation of the bleeding site was obtained in 20 out of 23 patients treated surgically. An intra-arterial vasoconstrictor infusion was given as a temporary measure before surgery in seven patients, only one of whom showed active bleeding at operation. An intra-arterial vasoconstrictor infusion was tried as definitive treatment in an additional 10 patients, but in four out of seven with a chronic ulcer bleeding recurred after 5-68 hours and was therefore treated surgically. We recommend the diagnostic use of arteriography in patients with reliable evidence of active bleeding if its site cannot be determined by endoscopy. We do not recommend its therapeutic use in those with a chronic ulcer, except to facilitate resuscitation before surgery; further studies are needed to define its role in those with an acute lesion."}
{"id": "PMID:1083757", "title": "Factor-VIII-related antigen: measurement by enzyme immunoassay.", "content": "Factor-VIII-related antigen was measured, both by an enzyme immunoassay using a microplate method and by the Laurell technique, in normal people, patients with von Willebrand's disease, haemophiliacs, and obligatory haemophilia carriers. The enzyme immunoassay was simpler to perform and gave equally reliable and reproducible results. Many more assays could be carried out at any one time.", "contents": "Factor-VIII-related antigen: measurement by enzyme immunoassay. Factor-VIII-related antigen was measured, both by an enzyme immunoassay using a microplate method and by the Laurell technique, in normal people, patients with von Willebrand's disease, haemophiliacs, and obligatory haemophilia carriers. The enzyme immunoassay was simpler to perform and gave equally reliable and reproducible results. Many more assays could be carried out at any one time."}
{"id": "PMID:1083759", "title": "Extra-articular features in early rheumatoid disease.", "content": "One hundred and two patients who presented with rheumatoid disease within the first year of onset were studied prospectively every four months for a mean 4.5 years to assess the incidence of extra-articular features. The features that seemed to be common in the early stages included hand-muscle wasting, carpal tunnel syndrome, lymphadenopathy, non-specific ankle swelling, and rheumatoid nodules, and to a lesser extent hepatomegaly, being underweight, conjunctivitis, skin transparency, and a palpable thyroid gland. Those features which seldom occurred early included scleromalacia, temporal artery inolvement, salivary gland enlargement, distal-motor neuropathy, splenomegaly, digital vasculitis, and pulmonary and cardiac complications. Being underweight indicated a significantly more severe outcome.", "contents": "Extra-articular features in early rheumatoid disease. One hundred and two patients who presented with rheumatoid disease within the first year of onset were studied prospectively every four months for a mean 4.5 years to assess the incidence of extra-articular features. The features that seemed to be common in the early stages included hand-muscle wasting, carpal tunnel syndrome, lymphadenopathy, non-specific ankle swelling, and rheumatoid nodules, and to a lesser extent hepatomegaly, being underweight, conjunctivitis, skin transparency, and a palpable thyroid gland. Those features which seldom occurred early included scleromalacia, temporal artery inolvement, salivary gland enlargement, distal-motor neuropathy, splenomegaly, digital vasculitis, and pulmonary and cardiac complications. Being underweight indicated a significantly more severe outcome."}
{"id": "PMID:1083760", "title": "Prognostic value of early features in rheumatoid disease.", "content": "Extensive data on 102 patients who presented with rheumatoid disease within a year of onset were gathered by a prospective study to assess the prognostic value of early features. Outcome was evaluated at a mean 4-5 years from onset on the basis of functional grade, extent of joint disease, early morning stiffness, and grip strength. Twenty-six patients improved, 14 pursued a mild steady course, and 62 had a persistently severe or deteriorating condition. The features recorded at the first visit were correlated with outcome. Those indicating a poor prognosis were: older age at onset, being underweight, poor grip strength, many affected joints, involvement of wrist or metatarsophalangeal joints, poor functional status, fulfilment of many of the American Rheumatism Association criteria for rheumatoid disease, raised erythrocyte sedimentation rate, seropositivity on sheep cell agglutination or latex tests, low haemoglobin level, raised blood urea level, and early erosions on x-ray films.", "contents": "Prognostic value of early features in rheumatoid disease. Extensive data on 102 patients who presented with rheumatoid disease within a year of onset were gathered by a prospective study to assess the prognostic value of early features. Outcome was evaluated at a mean 4-5 years from onset on the basis of functional grade, extent of joint disease, early morning stiffness, and grip strength. Twenty-six patients improved, 14 pursued a mild steady course, and 62 had a persistently severe or deteriorating condition. The features recorded at the first visit were correlated with outcome. Those indicating a poor prognosis were: older age at onset, being underweight, poor grip strength, many affected joints, involvement of wrist or metatarsophalangeal joints, poor functional status, fulfilment of many of the American Rheumatism Association criteria for rheumatoid disease, raised erythrocyte sedimentation rate, seropositivity on sheep cell agglutination or latex tests, low haemoglobin level, raised blood urea level, and early erosions on x-ray films."}
{"id": "PMID:1083761", "title": "The effects of 5-hydroxytryptophan and L-tryptophan on wakefulness and sleep patterns in the cat.", "content": "Sleep in cats was studied for 24 h after i.p. injections of D,L-5-hydroxytryptophan (5-HTP, 40 mg/kg) or L-tryptophan (200 mg/kg and 300 mg/kg) in two separate experiments. Total sleep, total slow wave sleep or deep slow wave sleep was not changed in any experiment. There was an increase of the awake, drowsy pattern over the whole recording period after both 5-HTP and tryptophan injections. An accentuated drowsy pattern, with high voltage 4 c/sec synchronous waves, but with no sleep spindles, was seen during the first hours after 5-HTP injection. REM sleep was completely absent during the first 6 h after 5-HTP and significantly reduced during the first 3 h after tryptophan. The similarity of the effects of 5-HTP and tryptophan suggests that they both act as serotonin precursors. It is also suggested that the serotonin precursors have a general deactivating effect on the waking state, and that they do not necessarily subserve a specific sleep-inducing or sleep-maintaining function.", "contents": "The effects of 5-hydroxytryptophan and L-tryptophan on wakefulness and sleep patterns in the cat. Sleep in cats was studied for 24 h after i.p. injections of D,L-5-hydroxytryptophan (5-HTP, 40 mg/kg) or L-tryptophan (200 mg/kg and 300 mg/kg) in two separate experiments. Total sleep, total slow wave sleep or deep slow wave sleep was not changed in any experiment. There was an increase of the awake, drowsy pattern over the whole recording period after both 5-HTP and tryptophan injections. An accentuated drowsy pattern, with high voltage 4 c/sec synchronous waves, but with no sleep spindles, was seen during the first hours after 5-HTP injection. REM sleep was completely absent during the first 6 h after 5-HTP and significantly reduced during the first 3 h after tryptophan. The similarity of the effects of 5-HTP and tryptophan suggests that they both act as serotonin precursors. It is also suggested that the serotonin precursors have a general deactivating effect on the waking state, and that they do not necessarily subserve a specific sleep-inducing or sleep-maintaining function."}
{"id": "PMID:1083762", "title": "Audiogenic seizures: relation to age and mechanisms of monoamine neurotransmission.", "content": "Mice with a genetically determined susceptibility to audiogenic seizures were utilized to analyze the ontogeny of central monoamine neurotransmission in relation to a behavior with age-specific properties. Levels of noradrenaline (NA), dopamine (DA), and 5-hydroxytryptamine (5-HT) were measured in forebrain and hindbrain regions at 14, 21, 28, and 42 days postnatal age in genetically sensitive or resistant strains of mice. An in vivo estimate of tyrosine and tryptophan hydroxylase activity was obtained at the same ages by following the accumulation of 3,4-dihydroxyphenylalanine (DOPA) and 5-hydroxytryptophan (5-HTP) respectively, after the administration of a centrally effective L-amino acid decarboxylase inhibitor (R04-4602, 800 mg/kg). At 14 days, there was a faster rate of accumulation of DOPA in both the forebrain and hindbrain of the sensitive mice compared to mice of the nonsensitive strain. At 21 days, the age of maximal sensitivity in the sensitive mice, the levels of NA were significantly lower in both regions of the sensitive mice, but the accumulation of DOPA was similar between strains at this age. There was also a slightly lower level of 5-HT in the forebrain of sensitive mice at 21 days accompanied by a slower rate of accumulation of 5-HTP in this region. In the hindbrain of the sensitive animals however, the rate of accumulation of 5-HTP was faster than in the sensitive strain. At 28 days, some impairment in mechanisms within NA-containing neurons in the sensitive mice was still apparent (including lower NA levels). At 42 days, there were no differences in amine levels, however, the levels of accumulated DOPA and 5-HTP were significantly lower in the sensitive strain. The results suggest that in the sensitive mice, developmental differences in mechanisms of monoamine storage and/or synthesis may exist which could contribute to deficient amounts of physiologically releaseable transmitter.", "contents": "Audiogenic seizures: relation to age and mechanisms of monoamine neurotransmission. Mice with a genetically determined susceptibility to audiogenic seizures were utilized to analyze the ontogeny of central monoamine neurotransmission in relation to a behavior with age-specific properties. Levels of noradrenaline (NA), dopamine (DA), and 5-hydroxytryptamine (5-HT) were measured in forebrain and hindbrain regions at 14, 21, 28, and 42 days postnatal age in genetically sensitive or resistant strains of mice. An in vivo estimate of tyrosine and tryptophan hydroxylase activity was obtained at the same ages by following the accumulation of 3,4-dihydroxyphenylalanine (DOPA) and 5-hydroxytryptophan (5-HTP) respectively, after the administration of a centrally effective L-amino acid decarboxylase inhibitor (R04-4602, 800 mg/kg). At 14 days, there was a faster rate of accumulation of DOPA in both the forebrain and hindbrain of the sensitive mice compared to mice of the nonsensitive strain. At 21 days, the age of maximal sensitivity in the sensitive mice, the levels of NA were significantly lower in both regions of the sensitive mice, but the accumulation of DOPA was similar between strains at this age. There was also a slightly lower level of 5-HT in the forebrain of sensitive mice at 21 days accompanied by a slower rate of accumulation of 5-HTP in this region. In the hindbrain of the sensitive animals however, the rate of accumulation of 5-HTP was faster than in the sensitive strain. At 28 days, some impairment in mechanisms within NA-containing neurons in the sensitive mice was still apparent (including lower NA levels). At 42 days, there were no differences in amine levels, however, the levels of accumulated DOPA and 5-HTP were significantly lower in the sensitive strain. The results suggest that in the sensitive mice, developmental differences in mechanisms of monoamine storage and/or synthesis may exist which could contribute to deficient amounts of physiologically releaseable transmitter."}
{"id": "PMID:1083765", "title": "Deoxynucleotide-polymerizing enzyme activities in T- and B-cells of acute lymphoblastic leukemia origin.", "content": "All 5 thymus-dependent cell (T-cell) lines (Molt-3; Molt-4; RPMI-8402; CCRF-CEM; CCRF-HSB-2) and 7 thymus-independent cell (B-cell) lines (RPMI-8382, RPMI-8392, RPMI-8412, RPMI-8422, RPMI-8432, RPMI-8442, CCRF-SB) established so far from acute lymphoblastic leukemia patients were examined for deoxynucleotide polymerizing enzymes. All T- and B-cells had DNA polymerase gamma, DNA polymerase beta, and terminal deoxynucleotidyl transferase both in the soluble (the latter 2 enzymes only in small amounts) and chromatin fraction, whereas DNA polymerase alpha was found only in the soluble fraction. With respect to their sedimentation and chromatographic behavior, template-primer requirements, Km for deoxythymidine triphosphate or deoxyguanosine triphosphate divalent cation preference, effect of NaCI and inhibitors, the enzymes from T- and B-cells resembled each other and those from other mammalian cells. DNA polymerase alpha, beta, and gamma from T-cells like those from \"fresh\" acute lymphoblastic leukemia cells, were more thermolabile than those from B-cells or phytohemagglutinin-stimulated normal lymphocytes. In addition, the terminal deoxynucleotidyl transferase from the above cells was completely inactivated in 5 to 6 min at 50 degrees, whereas the DNA polymerase alpha, beta, and gamma retained considerable activity even after heating for 25 min at 50 degrees. DNA polymerase activity of the soluble fraction from T-cells was of the same magnitude as in B-cells when expressed on a DNA basis but twice that of B-cells when expressed on a protein basis. High terminal deoxynucleotidyl transferase activity, equivalent to that observed in acute lymphoblastic leukemia cells, was found in all T-cell lines that, when expressed on a DNA basis, was 30 to 100 times higher than the B-cell lines tested. These results support the suggestion of earlier investigators that T-cell lines examined here may have originated from leukemic cells.", "contents": "Deoxynucleotide-polymerizing enzyme activities in T- and B-cells of acute lymphoblastic leukemia origin. All 5 thymus-dependent cell (T-cell) lines (Molt-3; Molt-4; RPMI-8402; CCRF-CEM; CCRF-HSB-2) and 7 thymus-independent cell (B-cell) lines (RPMI-8382, RPMI-8392, RPMI-8412, RPMI-8422, RPMI-8432, RPMI-8442, CCRF-SB) established so far from acute lymphoblastic leukemia patients were examined for deoxynucleotide polymerizing enzymes. All T- and B-cells had DNA polymerase gamma, DNA polymerase beta, and terminal deoxynucleotidyl transferase both in the soluble (the latter 2 enzymes only in small amounts) and chromatin fraction, whereas DNA polymerase alpha was found only in the soluble fraction. With respect to their sedimentation and chromatographic behavior, template-primer requirements, Km for deoxythymidine triphosphate or deoxyguanosine triphosphate divalent cation preference, effect of NaCI and inhibitors, the enzymes from T- and B-cells resembled each other and those from other mammalian cells. DNA polymerase alpha, beta, and gamma from T-cells like those from \"fresh\" acute lymphoblastic leukemia cells, were more thermolabile than those from B-cells or phytohemagglutinin-stimulated normal lymphocytes. In addition, the terminal deoxynucleotidyl transferase from the above cells was completely inactivated in 5 to 6 min at 50 degrees, whereas the DNA polymerase alpha, beta, and gamma retained considerable activity even after heating for 25 min at 50 degrees. DNA polymerase activity of the soluble fraction from T-cells was of the same magnitude as in B-cells when expressed on a DNA basis but twice that of B-cells when expressed on a protein basis. High terminal deoxynucleotidyl transferase activity, equivalent to that observed in acute lymphoblastic leukemia cells, was found in all T-cell lines that, when expressed on a DNA basis, was 30 to 100 times higher than the B-cell lines tested. These results support the suggestion of earlier investigators that T-cell lines examined here may have originated from leukemic cells."}
{"id": "PMID:1083766", "title": "Cytodynamics in the thymus of young adult mice: a quantitative study on the loss of thymic blast cells and non-proliferative small thymocytes.", "content": "Cell proliferation and cell loss in the thymic blast cell population were studied in young adult mice by (1) stathmokinetic methods combined with an analysis of the PLMe-curve after a pulse 3H-TdR, and (2) nigrosin-dye exclusion combined with 3H-TdR-autoradiography. It was calculated that about 17 percent of the blast cells do not progress into mitosis within the period of an average cell cycle. The dye exclusion studies indicated a rate of blast cell death of about 2-5 percent/hr. The two methods of assessing blast cell loss (death) support each other very well. In spite of these findings scintillation countings on thymuses removed from 1 to 17 hr after 3H-TdR injection showed fairly constant levels of thymic radioactivity. This suggests a very extensive reutilization of 3H-labelled break-down products from dying blast cells. The very sparse labelling of pyknotic thymocytes strongly suggests that thymic blast cells do not become pyknotic. The rate of small thymocyte production and disappearance was studied by pulse and repeated 3H-TdR labelling techniques combined with dye exclusion studies and pyknotic counts. The data from the repeated labelling experiment were analysed by use of a model based on the assumption of first order kinetics of small viable, dead, and pyknotic thymocytes. The rate of cell production was estimated to 1-6 percent/hr whereas the rates of cell loss due to disintegration, i.e. supravital stainability and nuclear pyknosis, were calculated to 0-02 percent/hr and 0-0006 percent/hr respectively. Cell loss due to disintegration was less than 2 percent of the total loss of small thymocytes. It was concluded that pyknotic counts are a useless method of assessing the cell death in the population of thymic blast cells and small thymocytes. On the basis of a model for thymocyte proliferation, production and loss it is suggested that about 45 percent of the small viable thymocytes re-enter the generative cell pool, whereas about 55 percent disappear by emigration.", "contents": "Cytodynamics in the thymus of young adult mice: a quantitative study on the loss of thymic blast cells and non-proliferative small thymocytes. Cell proliferation and cell loss in the thymic blast cell population were studied in young adult mice by (1) stathmokinetic methods combined with an analysis of the PLMe-curve after a pulse 3H-TdR, and (2) nigrosin-dye exclusion combined with 3H-TdR-autoradiography. It was calculated that about 17 percent of the blast cells do not progress into mitosis within the period of an average cell cycle. The dye exclusion studies indicated a rate of blast cell death of about 2-5 percent/hr. The two methods of assessing blast cell loss (death) support each other very well. In spite of these findings scintillation countings on thymuses removed from 1 to 17 hr after 3H-TdR injection showed fairly constant levels of thymic radioactivity. This suggests a very extensive reutilization of 3H-labelled break-down products from dying blast cells. The very sparse labelling of pyknotic thymocytes strongly suggests that thymic blast cells do not become pyknotic. The rate of small thymocyte production and disappearance was studied by pulse and repeated 3H-TdR labelling techniques combined with dye exclusion studies and pyknotic counts. The data from the repeated labelling experiment were analysed by use of a model based on the assumption of first order kinetics of small viable, dead, and pyknotic thymocytes. The rate of cell production was estimated to 1-6 percent/hr whereas the rates of cell loss due to disintegration, i.e. supravital stainability and nuclear pyknosis, were calculated to 0-02 percent/hr and 0-0006 percent/hr respectively. Cell loss due to disintegration was less than 2 percent of the total loss of small thymocytes. It was concluded that pyknotic counts are a useless method of assessing the cell death in the population of thymic blast cells and small thymocytes. On the basis of a model for thymocyte proliferation, production and loss it is suggested that about 45 percent of the small viable thymocytes re-enter the generative cell pool, whereas about 55 percent disappear by emigration."}
{"id": "PMID:1083767", "title": "The size, density, and relative area of chromatic granules (\"hydrogenosomes\") in Trichomonas vaginalis Donne from cultures in logarithmic and stationary growth.", "content": "Electron micrographs of Trichomonas vaginalis cells were examined morphometrically by means of an electronic image analyser. Cells from cultures in logarithmic growth contained smaller chromatic granules (\"hydrogenosomes\") and a higher proportion of sausage- or dumb-bell-shaped granules than did cells in stationary growth. These findings support the view that chromatic granules replicate by division of pre-existing granules and that this involves elongation and attenuation of their mid-region. The total area of cross sectioned granules occupied the same percentage of the cytoplasm of cells from cultures in logarithmic and stationary growth. The density of chromatic granules in the cytoplasm was identical for the two groups of cells.", "contents": "The size, density, and relative area of chromatic granules (\"hydrogenosomes\") in Trichomonas vaginalis Donne from cultures in logarithmic and stationary growth. Electron micrographs of Trichomonas vaginalis cells were examined morphometrically by means of an electronic image analyser. Cells from cultures in logarithmic growth contained smaller chromatic granules (\"hydrogenosomes\") and a higher proportion of sausage- or dumb-bell-shaped granules than did cells in stationary growth. These findings support the view that chromatic granules replicate by division of pre-existing granules and that this involves elongation and attenuation of their mid-region. The total area of cross sectioned granules occupied the same percentage of the cytoplasm of cells from cultures in logarithmic and stationary growth. The density of chromatic granules in the cytoplasm was identical for the two groups of cells."}
{"id": "PMID:1083768", "title": "Fenestrated blood capillaries in rat cranio-spinal sensory ganglia.", "content": "Several fenestrated capillaries were seen in the endoneurium of trigeminal and dorsal root ganglia from two young adult albino rats treated with tetraethylthiuram disulfide. The finding is regarded as normal, although the possibility exists that intoxication with tetraethylthiuram disulfide may have enhanced the intensity and/or rate of this cytologic specialization of some isolated endothelial cells.", "contents": "Fenestrated blood capillaries in rat cranio-spinal sensory ganglia. Several fenestrated capillaries were seen in the endoneurium of trigeminal and dorsal root ganglia from two young adult albino rats treated with tetraethylthiuram disulfide. The finding is regarded as normal, although the possibility exists that intoxication with tetraethylthiuram disulfide may have enhanced the intensity and/or rate of this cytologic specialization of some isolated endothelial cells."}
{"id": "PMID:1083781", "title": "Aortocoronary bypass grafting in a child with coronary artery obstruction due to mucocutaneous lymphnode syndrome: report of a case.", "content": "A four-year-old boy with a myocardial infarct and total occlusion of the right coronary and the left anterior descending coronary arteries due to mucocoutaneous lymphnode syndrome (MCLS). confirmed by selective coronary arteriography, underwent successful double aortocoronary bypass grafting. Patency of the grafts was demonstrated by graft angiography and the improvement of the contractile pattern of the left ventricle was reflected by the increase in ejection fraction from 0.45 to 0.61. This is the first patient successfully treated by aortocoronary bypass grafting for coronary artery obstructive lesions due to MCLS. This experience has demonstrated the feasibility of surgical management in a child with coronary artery obstruction due to MCLS. However, there are unanswered questions regarding the fate of the saphenous vein graft in relation to the growth of a child. Long-term clinical and angiographic follow-ups are mandatory to determine the significance of this mode of surgical treatment for the sequela of MCLS.", "contents": "Aortocoronary bypass grafting in a child with coronary artery obstruction due to mucocutaneous lymphnode syndrome: report of a case. A four-year-old boy with a myocardial infarct and total occlusion of the right coronary and the left anterior descending coronary arteries due to mucocoutaneous lymphnode syndrome (MCLS). confirmed by selective coronary arteriography, underwent successful double aortocoronary bypass grafting. Patency of the grafts was demonstrated by graft angiography and the improvement of the contractile pattern of the left ventricle was reflected by the increase in ejection fraction from 0.45 to 0.61. This is the first patient successfully treated by aortocoronary bypass grafting for coronary artery obstructive lesions due to MCLS. This experience has demonstrated the feasibility of surgical management in a child with coronary artery obstruction due to MCLS. However, there are unanswered questions regarding the fate of the saphenous vein graft in relation to the growth of a child. Long-term clinical and angiographic follow-ups are mandatory to determine the significance of this mode of surgical treatment for the sequela of MCLS."}
{"id": "PMID:1083782", "title": "Secondary immunodeficiency in miliary tuberculosis.", "content": "Cell-mediated immune response was investigated in fifteen patients with miliary tuberculosis. Delayed hypersensitivity skin test with \"recall\" antigens PPD and SKSD was positive in two and one patients respectively. An irritant dose of DNCB failed to induce non-specific inflammatory response in the skin of thirteen patients and the same patients also did not develop contact sensitivity to DNCB. Leucocyte migration test in the presence of Mycobacterium tuberculosis was also negative in eight of eleven patients studied. The proportion of E rosette-forming cells was found to be significantly depressed, though the proportion of EAC rosette-forming cells did not show any abnormality. On repeat skin tests in five patients after 3 months of chemotherapy and clinical improvement four showed a positive PPD and DNCB response. It was concluded that there is a marked degree of secondary immunodeficiency in miliary tuberculosis.", "contents": "Secondary immunodeficiency in miliary tuberculosis. Cell-mediated immune response was investigated in fifteen patients with miliary tuberculosis. Delayed hypersensitivity skin test with \"recall\" antigens PPD and SKSD was positive in two and one patients respectively. An irritant dose of DNCB failed to induce non-specific inflammatory response in the skin of thirteen patients and the same patients also did not develop contact sensitivity to DNCB. Leucocyte migration test in the presence of Mycobacterium tuberculosis was also negative in eight of eleven patients studied. The proportion of E rosette-forming cells was found to be significantly depressed, though the proportion of EAC rosette-forming cells did not show any abnormality. On repeat skin tests in five patients after 3 months of chemotherapy and clinical improvement four showed a positive PPD and DNCB response. It was concluded that there is a marked degree of secondary immunodeficiency in miliary tuberculosis."}
{"id": "PMID:1083783", "title": "Antibodies binding polyriboadenylic acid in systemic lupus erythematosus. Immunochemical characterization and isolation by affinity chromatography.", "content": "Antibodies specific for polyriboadenylic acid (poly rA) are present in sera from patients with systemic lupus erythematosus and from  NZB/NZW F1 mice. The specificity of these antibodies was established by inhibition of [3H]poly rA binding and by affinity chromatography. Poly rA binding was associated with the 19S and 7S regions when serum was fractionated by sucrose density gradient untracentrifugation. Young NZB/NZW F1 mice (1-5 months) had only 19S anti-poly rA, whereas old NZB/NZW F1 mice (2 years) had activity in both 19S and 7S regions, suggesting a possible age-dependent switching mechanism in the spontaneous development of antibodies to nucleic acids. The gamma-globulin fraction from an SLE patient was subjected to affinity chromatography on a column of poly rA covalently linked to Sepharose. An enriched population of IgG antibodies binding only poly rA, but not native or denatured DNA, was isolated in this manner. This procedure may have broad biological applicability for the preparation of isolated immunospecific anti-nucleic acid antibodies.", "contents": "Antibodies binding polyriboadenylic acid in systemic lupus erythematosus. Immunochemical characterization and isolation by affinity chromatography. Antibodies specific for polyriboadenylic acid (poly rA) are present in sera from patients with systemic lupus erythematosus and from  NZB/NZW F1 mice. The specificity of these antibodies was established by inhibition of [3H]poly rA binding and by affinity chromatography. Poly rA binding was associated with the 19S and 7S regions when serum was fractionated by sucrose density gradient untracentrifugation. Young NZB/NZW F1 mice (1-5 months) had only 19S anti-poly rA, whereas old NZB/NZW F1 mice (2 years) had activity in both 19S and 7S regions, suggesting a possible age-dependent switching mechanism in the spontaneous development of antibodies to nucleic acids. The gamma-globulin fraction from an SLE patient was subjected to affinity chromatography on a column of poly rA covalently linked to Sepharose. An enriched population of IgG antibodies binding only poly rA, but not native or denatured DNA, was isolated in this manner. This procedure may have broad biological applicability for the preparation of isolated immunospecific anti-nucleic acid antibodies."}
{"id": "PMID:1083784", "title": "Lymphocyte subpopulations, serum IgE and total eosinophil counts in patients with bronchial asthma.", "content": "Absolute T and B lymphocytes, total eosinophil counts (TEC), and serum IgE levels were studied in twenty-three asthmatic subjects. Compared to a mean peripheral T-lymphocyte count of 1620+/-486/mm3 in twenty control subjects, the mean value in the asthmatic patients was depressed significantly at 1192+/-4 12 (P less than 0-005). T lymphopenia was observed in ten of twenty-three patients (43%). The serum IgE was elevated above the upper limits of 507 mug/ml in six of seventeen patients. TEC above 250/mm3 was observed in twelve of seventeen subjects. T lymphopenia and hyper-immunoglobulinaemia E were each associated with hypereosinophilia. No correlation was observed between T lymphopenia and hyperimmunoglobulinaemia E.", "contents": "Lymphocyte subpopulations, serum IgE and total eosinophil counts in patients with bronchial asthma. Absolute T and B lymphocytes, total eosinophil counts (TEC), and serum IgE levels were studied in twenty-three asthmatic subjects. Compared to a mean peripheral T-lymphocyte count of 1620+/-486/mm3 in twenty control subjects, the mean value in the asthmatic patients was depressed significantly at 1192+/-4 12 (P less than 0-005). T lymphopenia was observed in ten of twenty-three patients (43%). The serum IgE was elevated above the upper limits of 507 mug/ml in six of seventeen patients. TEC above 250/mm3 was observed in twelve of seventeen subjects. T lymphopenia and hyper-immunoglobulinaemia E were each associated with hypereosinophilia. No correlation was observed between T lymphopenia and hyperimmunoglobulinaemia E."}
{"id": "PMID:1083785", "title": "Cell-mediated immunity in the rheumatoid diseases. I. Skin testing and mitogenic responses in sero-negative arthritides.", "content": "Cellular immunity has been investigated in patients with various kinds of sero-negative arthritis. The incidence of cutaneous response to recall antigen streptokinase-streptodornase (SK-SD), and the ability to mount a primary cutaneous response to dinitrochlorobenzene (DNCB) have been examined in patients with ankylosing spondylitis and psoriatic arthritis. The results were not significantly different from normal. In vitro lymphocyte transformation in the presence of phytohaemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM) has been measured using peripheral blood lymphocytes from patients with ankylosing spondylitis, psoriatic arthritis and Reiter's disease. In comparison with a control group, significantly reduced responses were found to a low dose of PHA in the ankylosing spondylitis and Reiter disease patients. Significant increase in response occurred to a high dose of PHA, in patients with psoriatic arthritis and Reiter's disease, and to PWM in Reiter's disease patients. The in vitro results in the ankylosing spondylitis, psoriatic arthritis and Reiter's disease patients suggest some abnormality in the T-cell population in sero-negative arthritis.", "contents": "Cell-mediated immunity in the rheumatoid diseases. I. Skin testing and mitogenic responses in sero-negative arthritides. Cellular immunity has been investigated in patients with various kinds of sero-negative arthritis. The incidence of cutaneous response to recall antigen streptokinase-streptodornase (SK-SD), and the ability to mount a primary cutaneous response to dinitrochlorobenzene (DNCB) have been examined in patients with ankylosing spondylitis and psoriatic arthritis. The results were not significantly different from normal. In vitro lymphocyte transformation in the presence of phytohaemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM) has been measured using peripheral blood lymphocytes from patients with ankylosing spondylitis, psoriatic arthritis and Reiter's disease. In comparison with a control group, significantly reduced responses were found to a low dose of PHA in the ankylosing spondylitis and Reiter disease patients. Significant increase in response occurred to a high dose of PHA, in patients with psoriatic arthritis and Reiter's disease, and to PWM in Reiter's disease patients. The in vitro results in the ankylosing spondylitis, psoriatic arthritis and Reiter's disease patients suggest some abnormality in the T-cell population in sero-negative arthritis."}
{"id": "PMID:1083786", "title": "In vitro stimulation of murine lymphoid cell cultures by levamisole.", "content": "Levamisole has been reported to act as an immunological adjuvant. Experiments reported here on the effect of this agent on a variety of murine lymphoid culture systems were designed to gain an insight into its mechanism of action. We have found levamisole to be a weak mitogen for mouse spleen cells producing a dose related response which peaks at 48 hr in culture. The drug acted to augment the response of spleen cells to sub-optimal concentrations of concanavalin A, but had no unusual effect on the lipopolysaccharide stimulation of B-cell DNA synthesis in vitro. Levamisole was directly stimulatory on enriched T-cell populations and was found to have two actions: (1) to stimulate a subpopulation of T cells and (2) to augment the response of suboptimal mitogen concentrations of concanavalin A. In addition, we have found that murine thymocytes stimulated by concanavalin A were greatly potentiated in the presence of levamisole, but this population of cells could not be stimulated directly by the drug.", "contents": "In vitro stimulation of murine lymphoid cell cultures by levamisole. Levamisole has been reported to act as an immunological adjuvant. Experiments reported here on the effect of this agent on a variety of murine lymphoid culture systems were designed to gain an insight into its mechanism of action. We have found levamisole to be a weak mitogen for mouse spleen cells producing a dose related response which peaks at 48 hr in culture. The drug acted to augment the response of spleen cells to sub-optimal concentrations of concanavalin A, but had no unusual effect on the lipopolysaccharide stimulation of B-cell DNA synthesis in vitro. Levamisole was directly stimulatory on enriched T-cell populations and was found to have two actions: (1) to stimulate a subpopulation of T cells and (2) to augment the response of suboptimal mitogen concentrations of concanavalin A. In addition, we have found that murine thymocytes stimulated by concanavalin A were greatly potentiated in the presence of levamisole, but this population of cells could not be stimulated directly by the drug."}
{"id": "PMID:1083787", "title": "Formation of auto-rosettes by peripheral blood lymphocytes.", "content": "A mean of 3-4% (0-5-19-5%) of the peripheral blood lymphocytes of normal adults were shown to bind three or more autologous erythrocytes in vitro to form auto-rosettes. Marked individual fluctuations were observed. Increased percentages were observed in patients with cancer, but not in other selected groups, including a group who had undergone thymectomy for myasthenia gravis. Auto-rosette formation was shown to be a property of T cells by the demonstration of (a) simultaneous binding of autologous and sheep erythrocytes, (b) non-inhibition of auto-rosette formation by anti-immunoglobulin, and (c) formation of auto-rosettes by mitogen-stimulated T blasts. Auto rosette formation is a property of high percentages of human thymocytes, and of lymphocytes treated with neuraminidase or stimulated to blast-cell trans-formation by phytomitogens. It is suggested that auto-rosette formation by these cells is related to their relatively low content of cell-coat sialic acid, as compared with untreated T lymphocytes. The possible influence of the cell coat on lymphocyte function is discussed briefly.", "contents": "Formation of auto-rosettes by peripheral blood lymphocytes. A mean of 3-4% (0-5-19-5%) of the peripheral blood lymphocytes of normal adults were shown to bind three or more autologous erythrocytes in vitro to form auto-rosettes. Marked individual fluctuations were observed. Increased percentages were observed in patients with cancer, but not in other selected groups, including a group who had undergone thymectomy for myasthenia gravis. Auto-rosette formation was shown to be a property of T cells by the demonstration of (a) simultaneous binding of autologous and sheep erythrocytes, (b) non-inhibition of auto-rosette formation by anti-immunoglobulin, and (c) formation of auto-rosettes by mitogen-stimulated T blasts. Auto rosette formation is a property of high percentages of human thymocytes, and of lymphocytes treated with neuraminidase or stimulated to blast-cell trans-formation by phytomitogens. It is suggested that auto-rosette formation by these cells is related to their relatively low content of cell-coat sialic acid, as compared with untreated T lymphocytes. The possible influence of the cell coat on lymphocyte function is discussed briefly."}
{"id": "PMID:1083790", "title": "Coronary artery aneurysm. A review of the literature with a report of 11 new cases.", "content": "In a prospective study, 11 (1.5 percent) of 742 patients had angiographically proven coronary artery aneurysms. The clinical picture was similar to that of patients with severe coronary artery disease. The coronary artery aneurysms were multiple and were associated with extensive coronary atherosclerosis in ten of the 11 patients. Left ventricular function was impaired when measured by end-diastolic pressure, end-diastolic volume, and ejection fraction. Segmental left ventricular contraction was severely abnormal. The abnormality of segmental contraction, distribution of coronary artery obstructions, an presence of collateral circulation were not different from other patients with severe occlusive coronary atherosclerosis. These 11 cases plus the 23 previously reported ante mortem form the total reported in world literature. The etiology of cornonary artery aneurysms is most commonly atherosclerosis (17/34, or 50 percent). The natural history of this condition is not known. Because of the severe atherosclerosis and poor distal-vessel run-off, most patients are not considered good surgical condidates; however, 15 patients have had coronary arterial surgery, and 13 have survived the immediate postoperative period with some improvement of symptoms.", "contents": "Coronary artery aneurysm. A review of the literature with a report of 11 new cases. In a prospective study, 11 (1.5 percent) of 742 patients had angiographically proven coronary artery aneurysms. The clinical picture was similar to that of patients with severe coronary artery disease. The coronary artery aneurysms were multiple and were associated with extensive coronary atherosclerosis in ten of the 11 patients. Left ventricular function was impaired when measured by end-diastolic pressure, end-diastolic volume, and ejection fraction. Segmental left ventricular contraction was severely abnormal. The abnormality of segmental contraction, distribution of coronary artery obstructions, an presence of collateral circulation were not different from other patients with severe occlusive coronary atherosclerosis. These 11 cases plus the 23 previously reported ante mortem form the total reported in world literature. The etiology of cornonary artery aneurysms is most commonly atherosclerosis (17/34, or 50 percent). The natural history of this condition is not known. Because of the severe atherosclerosis and poor distal-vessel run-off, most patients are not considered good surgical condidates; however, 15 patients have had coronary arterial surgery, and 13 have survived the immediate postoperative period with some improvement of symptoms."}
{"id": "PMID:1083791", "title": "Kartagener's syndrome with corrected transposition. Conducting system studies and coronary arterial occlusion complicating valvular replacement.", "content": "An 18-year-old man whose sister has classic Kartagener's syndrome was found to have sinusitis, bronchiectasis, and corrected transposition with normal visceral situs. Congenital complete heart block was secondary to absence of conducting-system pathways between a small posterior atrioventricular node and the transposed His bundle and bundle branches. No anterior atrioventricular node was present. Prosthetic valvular replacement of the left-sided (morphologic right) atrioventricular valve was complicated by coronary arterial occlusion by suture, with subsequent myocardial infarction. The case appears to represent an unusual variant of Kartagener's syndrome with the abnormality of laterality being expressed as corrected transposition.", "contents": "Kartagener's syndrome with corrected transposition. Conducting system studies and coronary arterial occlusion complicating valvular replacement. An 18-year-old man whose sister has classic Kartagener's syndrome was found to have sinusitis, bronchiectasis, and corrected transposition with normal visceral situs. Congenital complete heart block was secondary to absence of conducting-system pathways between a small posterior atrioventricular node and the transposed His bundle and bundle branches. No anterior atrioventricular node was present. Prosthetic valvular replacement of the left-sided (morphologic right) atrioventricular valve was complicated by coronary arterial occlusion by suture, with subsequent myocardial infarction. The case appears to represent an unusual variant of Kartagener's syndrome with the abnormality of laterality being expressed as corrected transposition."}
{"id": "PMID:1083792", "title": "The relative efficacy of vestibular-proprioceptive stimulation and the upright position in enhancing visual pursuit in neonates.", "content": "48 healthy neonates born to multiparae were randomly assigned to view a moving stimulus either in the horizontal or the upright position, with or without added vestibular stimulation and with or without pacifier sucking. The infant was shown a moving black line inside a strictly controlled visual environment provided by an apparatus which permitted horizontal and upright positioning and displacement of the infant. Visual tracking was recorded by a concealed TV camera positioned at a constant distance and angle from the infants' eyes in both positions. Quality of tracking during each of 4 trials was scored on a 7-point scale. Results indicate that vestibularproprioceptive stimulation provided to the infant either horizontally or semivertically significantly enhanced his visual tracking, whereas the upright position did not. Pacifier sucking also improved his performace.", "contents": "The relative efficacy of vestibular-proprioceptive stimulation and the upright position in enhancing visual pursuit in neonates. 48 healthy neonates born to multiparae were randomly assigned to view a moving stimulus either in the horizontal or the upright position, with or without added vestibular stimulation and with or without pacifier sucking. The infant was shown a moving black line inside a strictly controlled visual environment provided by an apparatus which permitted horizontal and upright positioning and displacement of the infant. Visual tracking was recorded by a concealed TV camera positioned at a constant distance and angle from the infants' eyes in both positions. Quality of tracking during each of 4 trials was scored on a 7-point scale. Results indicate that vestibularproprioceptive stimulation provided to the infant either horizontally or semivertically significantly enhanced his visual tracking, whereas the upright position did not. Pacifier sucking also improved his performace."}
{"id": "PMID:1083795", "title": "[Collagenous fibrillogenesis in the damaged arterial intima].", "content": "Development of fibromuscular hyperplasia of the intima (intimal thickening) as a characteristic reparative reaction of the intimal tissue was found in the carotid artery and abdominal aorta after an increased mechanical loading during experimental stenosis by means of optical and electronic microscopic examinations of the arterial wall. In children, whose death was caused by rheumatism at various phases of the disease in the coronary artery there was also focal diffuse intimal hyperplasia. Both in the experimental and in the rheumatic injuries of the arteries in the thickened intima there was more scarce infiltration with plasmatic proteins and lipids were discovered. An inference was made for interferention of serum insudation with collagenous synthesis in the damaged and reparative thickening of the arterial intima.", "contents": "[Collagenous fibrillogenesis in the damaged arterial intima]. Development of fibromuscular hyperplasia of the intima (intimal thickening) as a characteristic reparative reaction of the intimal tissue was found in the carotid artery and abdominal aorta after an increased mechanical loading during experimental stenosis by means of optical and electronic microscopic examinations of the arterial wall. In children, whose death was caused by rheumatism at various phases of the disease in the coronary artery there was also focal diffuse intimal hyperplasia. Both in the experimental and in the rheumatic injuries of the arteries in the thickened intima there was more scarce infiltration with plasmatic proteins and lipids were discovered. An inference was made for interferention of serum insudation with collagenous synthesis in the damaged and reparative thickening of the arterial intima."}
{"id": "PMID:1083797", "title": "Increase of serum free thyroxine after propylthiouracil in vitro or its peroral administration to rats.", "content": "After the addition of 6-propyl-2-thiouracil (PTU) (2.4 mg/ml) in vitro to the normal human serum a two fold increase of dialyzable free thyroxine fraction (%FT4) was found. The addition of the same PTU dose to the serum of intact Wistar rats caused up to 4 fold increase of this fraction. After the addition of increasing doses of PTU (40--400 ng/ml) a linear increase of FT4 was found in human serum, while in rat serum a nonlinear dose-response relationship was observed. After a single peroral administration of PTU to rats (42 mg PTU per animal) the serum PTU level reached the maximum (55 ng PTU/ml) after one hour and then decreased very slowly. The free thyroxine fraction also increased up to 150% of control value, but this maximum increase was observed one hour to later then the maximum PTU level. Then the values of %FT4 rapidly decreased to the initial value. Such effect of PTU in vivo was about twofold greater than that obtained in the rat serum in vitro under the same actual PTU level. The results were compared with similar effect of thiocyanate reported previously and it was shown that the effect of PTU both in vitro and in vivo is greater than that of equimolar levels of thiocyanate.", "contents": "Increase of serum free thyroxine after propylthiouracil in vitro or its peroral administration to rats. After the addition of 6-propyl-2-thiouracil (PTU) (2.4 mg/ml) in vitro to the normal human serum a two fold increase of dialyzable free thyroxine fraction (%FT4) was found. The addition of the same PTU dose to the serum of intact Wistar rats caused up to 4 fold increase of this fraction. After the addition of increasing doses of PTU (40--400 ng/ml) a linear increase of FT4 was found in human serum, while in rat serum a nonlinear dose-response relationship was observed. After a single peroral administration of PTU to rats (42 mg PTU per animal) the serum PTU level reached the maximum (55 ng PTU/ml) after one hour and then decreased very slowly. The free thyroxine fraction also increased up to 150% of control value, but this maximum increase was observed one hour to later then the maximum PTU level. Then the values of %FT4 rapidly decreased to the initial value. Such effect of PTU in vivo was about twofold greater than that obtained in the rat serum in vitro under the same actual PTU level. The results were compared with similar effect of thiocyanate reported previously and it was shown that the effect of PTU both in vitro and in vivo is greater than that of equimolar levels of thiocyanate."}
{"id": "PMID:1083798", "title": "Further studies on response of blood thyroid hormone level to its acute depletion by isovolemic exchange transfusion.", "content": "Groups of rats weighing about 400 g and fed low iodine diet for 16 days were injected with 125I- daily from day 8 to 14. Two days later their blood thyroid hormone was acutely depleted with an isovolemic exchange transfusion of hormone-free blood cell suspension. Relative changes of total plasma radioactivity and iodinated compounds were measured at frequent intervals, maintaining isovolemia of the recipient animal. In normal animals a post-transfusion decrease of total plasma radioactivity was related to the degree of exchange of total blood volume as was the post-transfusion increase to the initial level. No post-transfusion increase of total plasma radioactivity or labeled thyroxine was found in thyreidectomized animals. On the other hand, in normal animals a post-transfusion increase of plasma labelled thyroxine was observed. A linear increase of labeled iodide in plasma was found in both normal and thyroidectomized rats, the mechanism of which in thyroidectomized animals may be explained by a reflux of iodide from peripheral tissues to the blood. In normal animals a TSH-induced release of iodide from thyroid due to TSH action may participate in this phenomenon.", "contents": "Further studies on response of blood thyroid hormone level to its acute depletion by isovolemic exchange transfusion. Groups of rats weighing about 400 g and fed low iodine diet for 16 days were injected with 125I- daily from day 8 to 14. Two days later their blood thyroid hormone was acutely depleted with an isovolemic exchange transfusion of hormone-free blood cell suspension. Relative changes of total plasma radioactivity and iodinated compounds were measured at frequent intervals, maintaining isovolemia of the recipient animal. In normal animals a post-transfusion decrease of total plasma radioactivity was related to the degree of exchange of total blood volume as was the post-transfusion increase to the initial level. No post-transfusion increase of total plasma radioactivity or labeled thyroxine was found in thyreidectomized animals. On the other hand, in normal animals a post-transfusion increase of plasma labelled thyroxine was observed. A linear increase of labeled iodide in plasma was found in both normal and thyroidectomized rats, the mechanism of which in thyroidectomized animals may be explained by a reflux of iodide from peripheral tissues to the blood. In normal animals a TSH-induced release of iodide from thyroid due to TSH action may participate in this phenomenon."}
{"id": "PMID:1083799", "title": "Effect of gamma irradiation on biological activity of thyrotropin.", "content": "The biological activity of thyrotropin (TSH) was tested after sterilization by 0.5 and 12.5 Mrad of gamma irradiation. It was found that the biological activity (McKenzie's assay) of TSH irradiated in dry state was not affected during the first month after sterilization by doses of 0.5 and 2.5 Mrad. However, substantial decrease of TSH biological activity was observed 3--5 months after the irradiation, the lower activity being after the former dose. The irradiation of TSH by 12.5 Mrad in dry state and by 0.5 and 2.5 Mrad in solution resulted in a decrease of biological activity already during first month. The structural changes in the molecule of TSH were apparently not very extensive, since a decrease of disulfide bonds from 0.96 to 0.77 M per 1M of TSH was found immediately after the irradiation, while UV absorbancy and electrophoretic mobility on polyacrylamide gel electrophoresis were unaffected. These changes were followed by the decrease of TSH stability during storage in dry state. It is hypothesized that TSH molecule may be affected in beta subunit or in its connection with alpha.", "contents": "Effect of gamma irradiation on biological activity of thyrotropin. The biological activity of thyrotropin (TSH) was tested after sterilization by 0.5 and 12.5 Mrad of gamma irradiation. It was found that the biological activity (McKenzie's assay) of TSH irradiated in dry state was not affected during the first month after sterilization by doses of 0.5 and 2.5 Mrad. However, substantial decrease of TSH biological activity was observed 3--5 months after the irradiation, the lower activity being after the former dose. The irradiation of TSH by 12.5 Mrad in dry state and by 0.5 and 2.5 Mrad in solution resulted in a decrease of biological activity already during first month. The structural changes in the molecule of TSH were apparently not very extensive, since a decrease of disulfide bonds from 0.96 to 0.77 M per 1M of TSH was found immediately after the irradiation, while UV absorbancy and electrophoretic mobility on polyacrylamide gel electrophoresis were unaffected. These changes were followed by the decrease of TSH stability during storage in dry state. It is hypothesized that TSH molecule may be affected in beta subunit or in its connection with alpha."}
{"id": "PMID:1083800", "title": "Effect of vasotocin on plasma GH, free fatty acids and glucose in coho salmon (Oncorhynchus kisutch).", "content": "Coho salmon fry were injected intraperitoneally with 50 mul of saline containing either 15 or 150 mU arginine vasotocin. Blood was collected 30 min and 120 min post-injection for estimations of hematocrit, plasma glucose, free fatty acids and growth hormone. Vasotocin injections of 150 mU, unlike those of 15 mU, caused a lowering of the blood hematocrit. Plasma levels of glucose, free fatty acids and growth hormone increased following injections of 15 mU vasotocin. The higher concentration of vasotocin (150 mU) decreased plasma levels of glucose (at 120 min post-injection), free fatty acids (at 30 min post-injection) and growth hormone (at 120 min post-injection). It thus appears that vasotocin is an antidiuretic hormone in coho salmon and it is suggested that glucogenic and lipolytic effect of vasotocin are mediated via growth hormone.", "contents": "Effect of vasotocin on plasma GH, free fatty acids and glucose in coho salmon (Oncorhynchus kisutch). Coho salmon fry were injected intraperitoneally with 50 mul of saline containing either 15 or 150 mU arginine vasotocin. Blood was collected 30 min and 120 min post-injection for estimations of hematocrit, plasma glucose, free fatty acids and growth hormone. Vasotocin injections of 150 mU, unlike those of 15 mU, caused a lowering of the blood hematocrit. Plasma levels of glucose, free fatty acids and growth hormone increased following injections of 15 mU vasotocin. The higher concentration of vasotocin (150 mU) decreased plasma levels of glucose (at 120 min post-injection), free fatty acids (at 30 min post-injection) and growth hormone (at 120 min post-injection). It thus appears that vasotocin is an antidiuretic hormone in coho salmon and it is suggested that glucogenic and lipolytic effect of vasotocin are mediated via growth hormone."}
{"id": "PMID:1083801", "title": "18-hydroxylated steroids in human hypertension - clinical study.", "content": "The detection of two unknown urinary steroids called x and y in various patients was previously reported. Compound x was tentatively characterized as a derivative of dihydro-18-hydroxy-DOC with two additional polar groups. In this communication clinical observations of a set of 25 hypertensive patients are presented. Compound x alone was found in 4 cases, compound y alone 4 cases and both compound x and y in 5 cases. In several other cases, hypokalemia and/or a decreased urinary Na+/K+ ratio were found.", "contents": "18-hydroxylated steroids in human hypertension - clinical study. The detection of two unknown urinary steroids called x and y in various patients was previously reported. Compound x was tentatively characterized as a derivative of dihydro-18-hydroxy-DOC with two additional polar groups. In this communication clinical observations of a set of 25 hypertensive patients are presented. Compound x alone was found in 4 cases, compound y alone 4 cases and both compound x and y in 5 cases. In several other cases, hypokalemia and/or a decreased urinary Na+/K+ ratio were found."}
{"id": "PMID:1083802", "title": "Mineralocorticoid like effect of new 18-hydroxy-steroid isolated from urine of hypertensive patients.", "content": "The affinity of new urinary 18-hydroxy-steroid (Cp x) for mineralocorticoid receptors was estimated. When rat kidney slices were incubated with 2 x 10(-9) M 3H-aldosterone, Cp x (2 x 10(-5) M) was able to compete with aldosterone for the mineralocorticoid cytosol receptors. Cp x had low but significant affinity for mineralocorticoid receptor sites. This affinity was less than that obtained with 18-OH-progesterone. Moreover, in the adrenalectomized rats, the injection of Cp x induced a decrease of urinary Na+/K+ ratio which seems comparable to that obtained with 18-OH-progesterone.", "contents": "Mineralocorticoid like effect of new 18-hydroxy-steroid isolated from urine of hypertensive patients. The affinity of new urinary 18-hydroxy-steroid (Cp x) for mineralocorticoid receptors was estimated. When rat kidney slices were incubated with 2 x 10(-9) M 3H-aldosterone, Cp x (2 x 10(-5) M) was able to compete with aldosterone for the mineralocorticoid cytosol receptors. Cp x had low but significant affinity for mineralocorticoid receptor sites. This affinity was less than that obtained with 18-OH-progesterone. Moreover, in the adrenalectomized rats, the injection of Cp x induced a decrease of urinary Na+/K+ ratio which seems comparable to that obtained with 18-OH-progesterone."}
{"id": "PMID:1083803", "title": "Protracted milk-ejecting effect of some oxytocin analogues in rats.", "content": "Protracted effect of [2-0-methyltyrosine]-deamino-1-carba-oxytocin, [2-0-methyltyrosine]-oxytocin and its dimeric form was studied on the mammary gland of lactating rat in vivo. Biphasic course of biological response found for monomers and slow onset of response observed for dimer led to the assumption that the substances behaved like hormonogens.", "contents": "Protracted milk-ejecting effect of some oxytocin analogues in rats. Protracted effect of [2-0-methyltyrosine]-deamino-1-carba-oxytocin, [2-0-methyltyrosine]-oxytocin and its dimeric form was studied on the mammary gland of lactating rat in vivo. Biphasic course of biological response found for monomers and slow onset of response observed for dimer led to the assumption that the substances behaved like hormonogens."}
{"id": "PMID:1083804", "title": "Effect of calcitonin on fracture healing in rats.", "content": "The influence of calcitonin on the healing of a fracture of tibial diaphysis in young rats has been studied with the aid of 85Sr investigations. The animals were subdivided into four groups (with facture + calcitonin, with fracture and no calcitonin, without fracture but with calcitonin treatment and intact controls). During the experimental period up to the day 38, repeated applications of calcitonin and 85Sr along with measurements of different parameters followed. These parameters consisted of: total body weights, 85Sr contents in the fractured and opposite tibiae, total body radioactivity of 85Sr, the weights of dried bones dissected after the day 38, the weights of bone ashes and the specific activity of 85Sr in the ashes. Increased 85Sr-uptake was seen as a sign of increased mineralisation of the callus. Calcitonin suppressed the resorption of bone during the callus formation and enhanced the mineralisation of callus to some extent. The main influence of calcitonin was seen to reduce the whole-body metabolic response to a localized skeletal injury--the fracture. The possible importance of this finding for the clinical practice is discussed briefly.", "contents": "Effect of calcitonin on fracture healing in rats. The influence of calcitonin on the healing of a fracture of tibial diaphysis in young rats has been studied with the aid of 85Sr investigations. The animals were subdivided into four groups (with facture + calcitonin, with fracture and no calcitonin, without fracture but with calcitonin treatment and intact controls). During the experimental period up to the day 38, repeated applications of calcitonin and 85Sr along with measurements of different parameters followed. These parameters consisted of: total body weights, 85Sr contents in the fractured and opposite tibiae, total body radioactivity of 85Sr, the weights of dried bones dissected after the day 38, the weights of bone ashes and the specific activity of 85Sr in the ashes. Increased 85Sr-uptake was seen as a sign of increased mineralisation of the callus. Calcitonin suppressed the resorption of bone during the callus formation and enhanced the mineralisation of callus to some extent. The main influence of calcitonin was seen to reduce the whole-body metabolic response to a localized skeletal injury--the fracture. The possible importance of this finding for the clinical practice is discussed briefly."}
{"id": "PMID:1083805", "title": "Immunological investigations in two brothers with ataxia telangiectasia Louis-Bar.", "content": "Two of three brothers with the classical signs of ataxia telangiectasia were investigated for their immunological disorders at the ages of 13 and 16 years, respectively. The elder brother also suffers from autoimmune hemolytic anemia, a complication which has not yet been described in the course of ataxia telangiectasia. Immunological investigations made in both brothers showed a reduction in the number and function of T lymphocytes. The number of B lymphocytes was normal, among which there were cells staining for IgA, although serum IgA was absent. It seems possible that this phenomenon is caused by a disturbance in the process of maturation of lymphoid cells with a lack of differentiation into IgA-synthesizing plasma cells.", "contents": "Immunological investigations in two brothers with ataxia telangiectasia Louis-Bar. Two of three brothers with the classical signs of ataxia telangiectasia were investigated for their immunological disorders at the ages of 13 and 16 years, respectively. The elder brother also suffers from autoimmune hemolytic anemia, a complication which has not yet been described in the course of ataxia telangiectasia. Immunological investigations made in both brothers showed a reduction in the number and function of T lymphocytes. The number of B lymphocytes was normal, among which there were cells staining for IgA, although serum IgA was absent. It seems possible that this phenomenon is caused by a disturbance in the process of maturation of lymphoid cells with a lack of differentiation into IgA-synthesizing plasma cells."}
{"id": "PMID:1083808", "title": "Normal colony stimulating factor (CSF) production by bone marrow stromal cells and abnormal granulopoiesis with decreased CFUc in S1/S1d mice.", "content": "The concentration of CFUC and the production of stromal-derived CSF in the femora of S1/S1d mice were determined. There was a lower concentration CFUC and a smaller total number of nucleated cells in the femoral marrow of WCB6. S1/S1d (S1/S1d) mice than in WCB6. +/+/(+/+) mice or in C57B1/6. +/+ (C57Bl) mice. On the other hand stromal-derived CSF production by femora from S1/S1d mice did not differ significantly from that of +/+'s. These observations indicate that the microenvironmental defect of S1/S1d mice results in decreased growth of granulocytic precursors as well as those of erythroid and megakaryocytic cells. This is consistent with the reported decrease in multipotential stem cell proliferation. Stromal cell derived CSF production was normal and could not be implicated in the decreased production of granulocytic precursors.", "contents": "Normal colony stimulating factor (CSF) production by bone marrow stromal cells and abnormal granulopoiesis with decreased CFUc in S1/S1d mice. The concentration of CFUC and the production of stromal-derived CSF in the femora of S1/S1d mice were determined. There was a lower concentration CFUC and a smaller total number of nucleated cells in the femoral marrow of WCB6. S1/S1d (S1/S1d) mice than in WCB6. +/+/(+/+) mice or in C57B1/6. +/+ (C57Bl) mice. On the other hand stromal-derived CSF production by femora from S1/S1d mice did not differ significantly from that of +/+'s. These observations indicate that the microenvironmental defect of S1/S1d mice results in decreased growth of granulocytic precursors as well as those of erythroid and megakaryocytic cells. This is consistent with the reported decrease in multipotential stem cell proliferation. Stromal cell derived CSF production was normal and could not be implicated in the decreased production of granulocytic precursors."}
{"id": "PMID:1083809", "title": "The avian pineal organ. Distribution of exogenous indoleamines: a qualitative study of the rudimentary photoreceptor cells by electron microscopic radioautography.", "content": "The uptake of 5-hydroxytryptophan (3H-5-HTP) and serotonin (3H-5-HT) and their distribution have been demonstrated, at the ultrastructural level, in the secretory rudimentary photoreceptor cells (SRP cells), in the pineal organ of the parakeet and the duck. In vivo experiments. When the parakeets were injected with 3H-5-HTP alone or pretreated with drugs interfering with monoamine metabolism (e.g. R04-4602, nialamide) prior to injection of the label, the SRP cells always exhibited specific radioautographic reactions, in the regions of the dense secretory granules of the cell body and asynaptic pedicles. The labeling concentration avried quantitatively with time (interval between injection of the label and sacrifice) and drugs used. In vitro experiments. The uptake of 3H-5-HTP and 3H-5-HT in the pineal organ of the duck has also been studied, using an incubation procedure. The results with the two precursors confirmed those obtained \"in vivo\" with the parakeet. From these results it is suggested that the uptake of precursors in some compartments of SRP cells is qualitatively independent of: a) the experimental period (experiments performed in winter, spring, and summer; uptake of precursors between 11.00 a.m. and 12.00) b) the precursor (3H-5-HTP or 3H-5-HT) c) the observation times. It is concluded that the SRP cell, an important step in the cytological and functional evolution of the pineal organ (Collin, 1969, 1971), is responsible for the synthesis of 5-HT. The metabolism of 5-HT seems also possible in such cells.", "contents": "The avian pineal organ. Distribution of exogenous indoleamines: a qualitative study of the rudimentary photoreceptor cells by electron microscopic radioautography. The uptake of 5-hydroxytryptophan (3H-5-HTP) and serotonin (3H-5-HT) and their distribution have been demonstrated, at the ultrastructural level, in the secretory rudimentary photoreceptor cells (SRP cells), in the pineal organ of the parakeet and the duck. In vivo experiments. When the parakeets were injected with 3H-5-HTP alone or pretreated with drugs interfering with monoamine metabolism (e.g. R04-4602, nialamide) prior to injection of the label, the SRP cells always exhibited specific radioautographic reactions, in the regions of the dense secretory granules of the cell body and asynaptic pedicles. The labeling concentration avried quantitatively with time (interval between injection of the label and sacrifice) and drugs used. In vitro experiments. The uptake of 3H-5-HTP and 3H-5-HT in the pineal organ of the duck has also been studied, using an incubation procedure. The results with the two precursors confirmed those obtained \"in vivo\" with the parakeet. From these results it is suggested that the uptake of precursors in some compartments of SRP cells is qualitatively independent of: a) the experimental period (experiments performed in winter, spring, and summer; uptake of precursors between 11.00 a.m. and 12.00) b) the precursor (3H-5-HTP or 3H-5-HT) c) the observation times. It is concluded that the SRP cell, an important step in the cytological and functional evolution of the pineal organ (Collin, 1969, 1971), is responsible for the synthesis of 5-HT. The metabolism of 5-HT seems also possible in such cells."}
{"id": "PMID:1083820", "title": "Splanchnic hemodynamics in cirrhotic patients with esophageal varices and gastrointestinal bleeding.", "content": "Twenty cirrhotic patients with esophageal varices and an episode of gastrointestinal bleeding within the previous week underwent hemodynamic studies which used an indicator dilution technique based on selective catheterization of the splanchnic arteries and hepatic vein. Portasystemic shunting of splenic arterial flow averaged 95% (range 80 to 100%) in 17 patients with proved variceal bleeding, but only 8% in 3 who bled from another site. Superior mesenteric shunting averaged 70% (range 27 to 100%) in those who bled from varices, and no shunting was detectable in the nonvariceal bleeders. In at least 4 patients shunting was essentially complete from both beds. In 11 others, however, more than one-third of mesenteric flow still perfused the liver. The pressure gradient from the wedge to free position in the hepatic vein and the hepatic blood flow bore no relationship to the degree of shunting. These data indicated the variability of the splanchnic hemodynamic pattern that may exist in alcoholic patients with esophageal varices. If hemodynamic factors are important in determining the response to surgery, indicator dilution studies to quantitate portasystemic shunting may be a vital part of preoperative evaluation.", "contents": "Splanchnic hemodynamics in cirrhotic patients with esophageal varices and gastrointestinal bleeding. Twenty cirrhotic patients with esophageal varices and an episode of gastrointestinal bleeding within the previous week underwent hemodynamic studies which used an indicator dilution technique based on selective catheterization of the splanchnic arteries and hepatic vein. Portasystemic shunting of splenic arterial flow averaged 95% (range 80 to 100%) in 17 patients with proved variceal bleeding, but only 8% in 3 who bled from another site. Superior mesenteric shunting averaged 70% (range 27 to 100%) in those who bled from varices, and no shunting was detectable in the nonvariceal bleeders. In at least 4 patients shunting was essentially complete from both beds. In 11 others, however, more than one-third of mesenteric flow still perfused the liver. The pressure gradient from the wedge to free position in the hepatic vein and the hepatic blood flow bore no relationship to the degree of shunting. These data indicated the variability of the splanchnic hemodynamic pattern that may exist in alcoholic patients with esophageal varices. If hemodynamic factors are important in determining the response to surgery, indicator dilution studies to quantitate portasystemic shunting may be a vital part of preoperative evaluation."}
{"id": "PMID:1083823", "title": "Effect of prostaglandin 15(R) 15 methyl-E2 methyl ester on aspirin and taurocholic acid-induced gastric mucosal haemorrhage in rats.", "content": "The effect of orally administered prostaglandin 15(R)15 methyl-E2 methyl ester on aspirin and taurocholic acid-induced gastric mucosal haemorrhage has been studied in rats. The incidence of haemorrhage induced by aspirin (26-7 mM), 64 mg/kg, together with taurocholic acid (2-5 mM), was significantly reduced from 53-6% to 19-5% by the addition of the prostaglandin (9-9 muM), P less than 0.01. The incidence of haemorrhage induced by aspirin alone (53-3 mM), 128 mg/kg, was significantly reduced from 80% to 20% by the addition of prostaglandin (9-9 muM), P less than 0.002. These results indicate the possible use of synthetic prostaglandins in the prevention of aspirin-induced gastric pathology.", "contents": "Effect of prostaglandin 15(R) 15 methyl-E2 methyl ester on aspirin and taurocholic acid-induced gastric mucosal haemorrhage in rats. The effect of orally administered prostaglandin 15(R)15 methyl-E2 methyl ester on aspirin and taurocholic acid-induced gastric mucosal haemorrhage has been studied in rats. The incidence of haemorrhage induced by aspirin (26-7 mM), 64 mg/kg, together with taurocholic acid (2-5 mM), was significantly reduced from 53-6% to 19-5% by the addition of the prostaglandin (9-9 muM), P less than 0.01. The incidence of haemorrhage induced by aspirin alone (53-3 mM), 128 mg/kg, was significantly reduced from 80% to 20% by the addition of prostaglandin (9-9 muM), P less than 0.002. These results indicate the possible use of synthetic prostaglandins in the prevention of aspirin-induced gastric pathology."}
{"id": "PMID:1083824", "title": "Upper gastrointestinal bleeding in cirrhosis: clinical and endoscopic correlations.", "content": "The clinical data of 180 episodes of upper gastrointestinal bleeding in 168 patients with cirrhosis of the liver are examined. The source of bleeding had been determined by early endoscopy in all cases. In men under the age of 50 years, and without symptoms of liver failure, bleeding was due to ruptured gastro-oesophageal varices in 84% of cases. Severe liver failure was associated with acute lesions of gastric mucosa in many cases. No presumptive diagnosis of the source of haemorrhage could be based on the examination of other clinical data (presence of ascites, mode of presentation and pattern of bleeding, history of ulcer disease, alcoholism, and previous medication.", "contents": "Upper gastrointestinal bleeding in cirrhosis: clinical and endoscopic correlations. The clinical data of 180 episodes of upper gastrointestinal bleeding in 168 patients with cirrhosis of the liver are examined. The source of bleeding had been determined by early endoscopy in all cases. In men under the age of 50 years, and without symptoms of liver failure, bleeding was due to ruptured gastro-oesophageal varices in 84% of cases. Severe liver failure was associated with acute lesions of gastric mucosa in many cases. No presumptive diagnosis of the source of haemorrhage could be based on the examination of other clinical data (presence of ascites, mode of presentation and pattern of bleeding, history of ulcer disease, alcoholism, and previous medication."}
{"id": "PMID:1083825", "title": "Comparison of emergent endoscopy and upper gastrointestinal series radiography in acute upper gastrointestinal haemorrhage.", "content": "A prospective study of early diagnostic procedures in acute upper gastrointestinal haemorrhage was conducted in a series of 76 patients. The diagnostic procedures included upper gastrointestinal series radiography (UGIS) and endoscopy (ENDO). The clinicians' diagnosis and management improved in a statistically significant way as a result of the findings of endoscopy. The findings of UGIS did not significantly improve diagnostic accuracy and resulted in a statistically significant adverse effect on patient management. The results suggest that endoscopy is more effective in promoting early accurate diagnosis and management in patients with acute upper gastrointestinal haemorrhage.", "contents": "Comparison of emergent endoscopy and upper gastrointestinal series radiography in acute upper gastrointestinal haemorrhage. A prospective study of early diagnostic procedures in acute upper gastrointestinal haemorrhage was conducted in a series of 76 patients. The diagnostic procedures included upper gastrointestinal series radiography (UGIS) and endoscopy (ENDO). The clinicians' diagnosis and management improved in a statistically significant way as a result of the findings of endoscopy. The findings of UGIS did not significantly improve diagnostic accuracy and resulted in a statistically significant adverse effect on patient management. The results suggest that endoscopy is more effective in promoting early accurate diagnosis and management in patients with acute upper gastrointestinal haemorrhage."}
{"id": "PMID:1083826", "title": "Polymorphism of alpha1-antitrypsin in a Portuguese population.", "content": "Serum Pi phenotypes were studied in 219 samples. The MM phenotype was the most common as in all other populations. The frequencies of PiS and PiZ were high considering other populations. PiF was not detected.", "contents": "Polymorphism of alpha1-antitrypsin in a Portuguese population. Serum Pi phenotypes were studied in 219 samples. The MM phenotype was the most common as in all other populations. The frequencies of PiS and PiZ were high considering other populations. PiF was not detected."}
{"id": "PMID:1083827", "title": "[Evidence for reduced colour vision in carriers of congenital colour vision deficiencies (author's transl)].", "content": "The ability to recognize small spots of coloured light in parafoveal regions of the retina was investigated in women heterozygous for protanopia (2 subjects), deuteranopia (2 subjects), or deuteranomaly (1 subject) and in 3 normal subjects. The homozygous colour normals had excellent discrimination up to 8 degrees excentricity, whereas in heterozygous carriers of congenital colour vision deficiencies the ability to differentiate colours varied from point to point within the retina. The results may be explained by assuming the existence of alternating patches of trichromatic and dichromatic cell populations with the retina of the heterozygous carriers.", "contents": "[Evidence for reduced colour vision in carriers of congenital colour vision deficiencies (author's transl)]. The ability to recognize small spots of coloured light in parafoveal regions of the retina was investigated in women heterozygous for protanopia (2 subjects), deuteranopia (2 subjects), or deuteranomaly (1 subject) and in 3 normal subjects. The homozygous colour normals had excellent discrimination up to 8 degrees excentricity, whereas in heterozygous carriers of congenital colour vision deficiencies the ability to differentiate colours varied from point to point within the retina. The results may be explained by assuming the existence of alternating patches of trichromatic and dichromatic cell populations with the retina of the heterozygous carriers."}
{"id": "PMID:1083828", "title": "Changes in thymocyte reactivity to lectins induced by B-cell mitogens of the type of sulphated polyanions.", "content": "Dextran sulphate, polyvinyl sulphate and carrageenan (but not the other polyanions tested, such as poly I:poly C, poly-L-glutamic acid or E. coli lipopolysaccharide) act synergisticaly with PHA on mouse thymocyte stimulation, as measured by thymidine uptake and blast cell transformation. Furthermore the active compounds shift the dose response of thymocytes to Con A. The effect could be observed in four different strains of mice tested by using normal or cortisone-resistant thymocytes. Two possibilities are envisaged for explanation of these effects: (a) attachment of polyanions by their anionic groups to cell surface constituents and interaction of the sulphate groups of the polymers with plant lectins; and (b) modification of the cell membrane induced by sulphated polymers changing either the binding capacity or the effectiveness of the membrane-associated events leading to thymocyte stimulation by the lectins.", "contents": "Changes in thymocyte reactivity to lectins induced by B-cell mitogens of the type of sulphated polyanions. Dextran sulphate, polyvinyl sulphate and carrageenan (but not the other polyanions tested, such as poly I:poly C, poly-L-glutamic acid or E. coli lipopolysaccharide) act synergisticaly with PHA on mouse thymocyte stimulation, as measured by thymidine uptake and blast cell transformation. Furthermore the active compounds shift the dose response of thymocytes to Con A. The effect could be observed in four different strains of mice tested by using normal or cortisone-resistant thymocytes. Two possibilities are envisaged for explanation of these effects: (a) attachment of polyanions by their anionic groups to cell surface constituents and interaction of the sulphate groups of the polymers with plant lectins; and (b) modification of the cell membrane induced by sulphated polymers changing either the binding capacity or the effectiveness of the membrane-associated events leading to thymocyte stimulation by the lectins."}
{"id": "PMID:1083829", "title": "Rabbit lymphoid cells. I. T-cell mitogens, cell volume and adherence properties as probes for cellular heterogeneity.", "content": "Thymus-derived cell populations were characterized by sedimentation velocity in the Earth's gravitational field, by adherence properties, and in terms of the thymus-specific antigen RTLA. T cells, responding to mitogens, could be shown to be a subpopulation of RTLA-bearing cells and to have a relatively large volume. The size distribution of mitogen-responsive cells from different individuals showed some variation, but in general large spleen cells took up less thymidine per 10(6) cells than did large cells from other lymphoid organs. Upon stimulation with concanavalin A (con A) small popliteal lymph node cells took up more thymidine per 10(6) cells than did small thymus cells. The corresponding small cells of mesenteric lymph node and spleen took up intermediate quantities of thymidine. Thymus-derived cells that were resistant to complement-mediated cell kill with RTLA antiserum showed greater responsiveness to con A than did the original cell preparations. Non-adherent cells incorporated more thymidine when stimulated with phytohaemagglutinin (PHA) than when they were stimulated with con A. Lightly adherent cells were relatively more responsive to con A stimulation and firmly adherent cells were less responsive to both. The findings were discussed in terms of the density of RTLA on the membranes of T cells. In adherence and in sedimentation velocity fractionation, the relative yield of nucleated cells always exceeded the yield of PHA-  and con A-responsive cells. The differential separation of mitogen responsive and of co-operating cells were considered as a possible cause of this deficit.", "contents": "Rabbit lymphoid cells. I. T-cell mitogens, cell volume and adherence properties as probes for cellular heterogeneity. Thymus-derived cell populations were characterized by sedimentation velocity in the Earth's gravitational field, by adherence properties, and in terms of the thymus-specific antigen RTLA. T cells, responding to mitogens, could be shown to be a subpopulation of RTLA-bearing cells and to have a relatively large volume. The size distribution of mitogen-responsive cells from different individuals showed some variation, but in general large spleen cells took up less thymidine per 10(6) cells than did large cells from other lymphoid organs. Upon stimulation with concanavalin A (con A) small popliteal lymph node cells took up more thymidine per 10(6) cells than did small thymus cells. The corresponding small cells of mesenteric lymph node and spleen took up intermediate quantities of thymidine. Thymus-derived cells that were resistant to complement-mediated cell kill with RTLA antiserum showed greater responsiveness to con A than did the original cell preparations. Non-adherent cells incorporated more thymidine when stimulated with phytohaemagglutinin (PHA) than when they were stimulated with con A. Lightly adherent cells were relatively more responsive to con A stimulation and firmly adherent cells were less responsive to both. The findings were discussed in terms of the density of RTLA on the membranes of T cells. In adherence and in sedimentation velocity fractionation, the relative yield of nucleated cells always exceeded the yield of PHA-  and con A-responsive cells. The differential separation of mitogen responsive and of co-operating cells were considered as a possible cause of this deficit."}
{"id": "PMID:1083830", "title": "Role of membrane structures in B-lymphocyte activation. The effect of binding anti-immunoglobulin, aggregated IgG and immune complexes.", "content": "It is shown that the binding of bivalent anti-immunoglobulin preparations to the surface immunoglobulin of murine B lymphocytes as well as the binding of aggregates of IgG or immune complexes does not lead to detectable increase in cell division in microcultures. Treating lymphocytes with immune complexes and aggregated IgG does not abolish the subsequent mitogenic response induced by LPS. The background mitosis observed in culture is inhibited with anti-immunoglobulin antibodies.", "contents": "Role of membrane structures in B-lymphocyte activation. The effect of binding anti-immunoglobulin, aggregated IgG and immune complexes. It is shown that the binding of bivalent anti-immunoglobulin preparations to the surface immunoglobulin of murine B lymphocytes as well as the binding of aggregates of IgG or immune complexes does not lead to detectable increase in cell division in microcultures. Treating lymphocytes with immune complexes and aggregated IgG does not abolish the subsequent mitogenic response induced by LPS. The background mitosis observed in culture is inhibited with anti-immunoglobulin antibodies."}
{"id": "PMID:1083831", "title": "Central and peripheral action of suppressor cells in contact sensitivity in the guinea-pig.", "content": "Suppressor cells were demonstrated in the spleen of guinea-pigs made specifically unresponsive to dinitrofluorobenzene (DNFB) with dinitrobenzene sulphonic acid (DNBSO3). Transfusion of these cells at the same time as sensitization with DNFB, produced a significant reduction in the immunoblasts proliferating in the draining lymph node 4 days later. Transfusion on the day of skin testing produced no greater suppression of skin reactivity than cells taken from animals made hypo-reactive to DNFB by contact with dinitrothiocyanate benzene (DNTB). It is concluded that there are at least two sites that suppressor cells can act. In the case of total unresponsiveness induced by DNBSO3, action is both central and in the periphery. In the case of hyporeactivity induced by DNTB, in which there is no defect in proliferation of T cells in response to antigen, the action of these cells is confined to the periphery. results of spleen weight studies suggest that suppressor cells homing in the spleen respond by proliferation to epicutaneously applied DNFB.", "contents": "Central and peripheral action of suppressor cells in contact sensitivity in the guinea-pig. Suppressor cells were demonstrated in the spleen of guinea-pigs made specifically unresponsive to dinitrofluorobenzene (DNFB) with dinitrobenzene sulphonic acid (DNBSO3). Transfusion of these cells at the same time as sensitization with DNFB, produced a significant reduction in the immunoblasts proliferating in the draining lymph node 4 days later. Transfusion on the day of skin testing produced no greater suppression of skin reactivity than cells taken from animals made hypo-reactive to DNFB by contact with dinitrothiocyanate benzene (DNTB). It is concluded that there are at least two sites that suppressor cells can act. In the case of total unresponsiveness induced by DNBSO3, action is both central and in the periphery. In the case of hyporeactivity induced by DNTB, in which there is no defect in proliferation of T cells in response to antigen, the action of these cells is confined to the periphery. results of spleen weight studies suggest that suppressor cells homing in the spleen respond by proliferation to epicutaneously applied DNFB."}
{"id": "PMID:1083833", "title": "Selective effect on T and B cell subpopulations in rat lymphoid organs after urethan treatment.", "content": "In vitro phytohemagglutinin (PHA) and concanavalin A (Con A) responsiveness of thymus and bone marrow cells, and E, EA, and EAC rosette-forming cells (E, EA, EAC) of these organs and spleen were studied in Fisher rats at various time intervals following a carcinogenic treatment with urethan (UR). Immediately after treatment, organ cellularities were drastically reduced with a progressive recovery as time passed. 1 day after treatment, the response of thymocytes to PHA showed a fourfold increase while organ cellularity dropped to 1%. This response fell below normal values after 7 days with an overshoot after days 14 and 21. UR-treated bone marrow cells showed a response to PHA below normal values after 1 and 7 days, a twofold increase after 3 days and an overshoot after 14 and 21 days. The responsiveness to Con A of both organs was affected by UR treatment to a much lesser extent, although following a pattern similar to that of PHA. E were never found, whether in normal nor in UR-treated animals. As far as the B-cell compartment is concerned, UR causes a progressive diminution of EAC, while the number of EA remains unaffected. The data are discussed in terms of selective effect on T- and B-cell subpopulations.", "contents": "Selective effect on T and B cell subpopulations in rat lymphoid organs after urethan treatment. In vitro phytohemagglutinin (PHA) and concanavalin A (Con A) responsiveness of thymus and bone marrow cells, and E, EA, and EAC rosette-forming cells (E, EA, EAC) of these organs and spleen were studied in Fisher rats at various time intervals following a carcinogenic treatment with urethan (UR). Immediately after treatment, organ cellularities were drastically reduced with a progressive recovery as time passed. 1 day after treatment, the response of thymocytes to PHA showed a fourfold increase while organ cellularity dropped to 1%. This response fell below normal values after 7 days with an overshoot after days 14 and 21. UR-treated bone marrow cells showed a response to PHA below normal values after 1 and 7 days, a twofold increase after 3 days and an overshoot after 14 and 21 days. The responsiveness to Con A of both organs was affected by UR treatment to a much lesser extent, although following a pattern similar to that of PHA. E were never found, whether in normal nor in UR-treated animals. As far as the B-cell compartment is concerned, UR causes a progressive diminution of EAC, while the number of EA remains unaffected. The data are discussed in terms of selective effect on T- and B-cell subpopulations."}
{"id": "PMID:1083834", "title": "Effects of cyclophosphamide treatment on the in vitro activity of mouse lymphoid cells after nonspecific and specific stimulation.", "content": "A single injection of 300 mg cyclophosphamide (CY)/kg resulted in the mouse in a reduction of the numbers of lymphocytes. The minimal values for spleen and peripheral lymph nodes (PLN) were about 10% and were reached on day 3. The restoration of the spleen started on day 5, while the number of PLN cells was still low on day 21. CY treatment followed by immunization resulted in accelerated recovery and considerably enhanced cell numbers in both organs. Staining with fluorescent antisera revealed that, although the B cell is the main target cell of CY action, in the spleen the total percentage of B and T cells decreases during the period in which the total cell number increases. Immunization diminished the in vitro responsiveness of spleen and PLN cells on phytohemagglutinin (PHA) and concanavalin A (Con A), 7 and 11 days later. The response of PLN cells on lipopolysaccharide (LPS) was, however, strongly enhanced on day 7. CY pretreatment of normal and immunized mice yielded cell populations which behaved identically on nonspecific stimulation in vitro. Starting 5 days after CY, the PHA and Con A responses for spleen cells were impaired, while the initial low R values for PLN cells showed a steady increase. Stimulation of CY spleen cells with LPS resulted in R values of below 1. Specific in vitro stimulation of lymphocytes of immunized mice was performed with sheep red blood cells and a hapten-carrier complex. CY treatment before immunization resulted in an elimination of the in vitro activity on specific stimulation.", "contents": "Effects of cyclophosphamide treatment on the in vitro activity of mouse lymphoid cells after nonspecific and specific stimulation. A single injection of 300 mg cyclophosphamide (CY)/kg resulted in the mouse in a reduction of the numbers of lymphocytes. The minimal values for spleen and peripheral lymph nodes (PLN) were about 10% and were reached on day 3. The restoration of the spleen started on day 5, while the number of PLN cells was still low on day 21. CY treatment followed by immunization resulted in accelerated recovery and considerably enhanced cell numbers in both organs. Staining with fluorescent antisera revealed that, although the B cell is the main target cell of CY action, in the spleen the total percentage of B and T cells decreases during the period in which the total cell number increases. Immunization diminished the in vitro responsiveness of spleen and PLN cells on phytohemagglutinin (PHA) and concanavalin A (Con A), 7 and 11 days later. The response of PLN cells on lipopolysaccharide (LPS) was, however, strongly enhanced on day 7. CY pretreatment of normal and immunized mice yielded cell populations which behaved identically on nonspecific stimulation in vitro. Starting 5 days after CY, the PHA and Con A responses for spleen cells were impaired, while the initial low R values for PLN cells showed a steady increase. Stimulation of CY spleen cells with LPS resulted in R values of below 1. Specific in vitro stimulation of lymphocytes of immunized mice was performed with sheep red blood cells and a hapten-carrier complex. CY treatment before immunization resulted in an elimination of the in vitro activity on specific stimulation."}
{"id": "PMID:1083835", "title": "T lymphocytes in atopic children.", "content": "The number of circulating T and B cells and the sensitivity of lymphocytes to stimulation with phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweek mitogen (PWM) was studied in 233 atopic children. The number of T lymphocytes was found to be decreased in cases of rhinoconjunctivitis, asthma as well as atopic eczema. Levels of B lymphocytes were normal. Sensitivity to stimulation with PHA and to a lesser degree, Con A, was significantly decreased whereas stimulation with PWM was unaffected. The severity of the atopic eczema was inversely correlated to T cell numbers. Several lines of evidence indicated that the abnormalities observed were intrinsically associated with the atopic conditions and not evoked by corticosteroid treatment. The results are compatible with the hypothesis that atopy is associated with a defect of a subpopulation of T cells. The possibility that this subpopulation has a suppressor function on reagin formation is discussed.", "contents": "T lymphocytes in atopic children. The number of circulating T and B cells and the sensitivity of lymphocytes to stimulation with phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweek mitogen (PWM) was studied in 233 atopic children. The number of T lymphocytes was found to be decreased in cases of rhinoconjunctivitis, asthma as well as atopic eczema. Levels of B lymphocytes were normal. Sensitivity to stimulation with PHA and to a lesser degree, Con A, was significantly decreased whereas stimulation with PWM was unaffected. The severity of the atopic eczema was inversely correlated to T cell numbers. Several lines of evidence indicated that the abnormalities observed were intrinsically associated with the atopic conditions and not evoked by corticosteroid treatment. The results are compatible with the hypothesis that atopy is associated with a defect of a subpopulation of T cells. The possibility that this subpopulation has a suppressor function on reagin formation is discussed."}
{"id": "PMID:1083836", "title": "Allergic contact dermatitis in the B-cell deficient chicken.", "content": "Allergic contact dermatitis to oxazolone was induced in three chickens rendered B-cell deficient by combined chemical bursectomy with testosterone and cyclophosphamide.", "contents": "Allergic contact dermatitis in the B-cell deficient chicken. Allergic contact dermatitis to oxazolone was induced in three chickens rendered B-cell deficient by combined chemical bursectomy with testosterone and cyclophosphamide."}
{"id": "PMID:1083837", "title": "Flash photolysis of enzymes.", "content": "The photoionization of aromatic residues constitutes a major initial photochemical reaction in the flash photolysis of proteins at gamma greater than 250 nm. The ejected electrons have been observed as eaq- and the disulphide bridge electron adduct, and also must be trapped at unidentified sites. The number of tryptophyl (or tyrosyl) residues photo-ionized at 5 musec delay is approximately equal to the number of exposed residues. The flash photolysis data have been related to inactivation by considering how photolysis of these \"photolabile\" residues can affect enzymic activity, based on the microstructure and available information about permanent alterations and residue specificities. This analysis indicates that hen lysozyme and papain are inactivated by photolysis of an essential Trp residue, that bovine trypsin is inactivated by photolysis of a Trp residue adjacent to the key catalytic Ser and other pathways initiated by excitation of Tyr and Cys, that the efficient photoionization of Tyr and RNase A is not an important inactivating reaction, and that aromatic residues in subtilisn Carlsberg are photosensitive.", "contents": "Flash photolysis of enzymes. The photoionization of aromatic residues constitutes a major initial photochemical reaction in the flash photolysis of proteins at gamma greater than 250 nm. The ejected electrons have been observed as eaq- and the disulphide bridge electron adduct, and also must be trapped at unidentified sites. The number of tryptophyl (or tyrosyl) residues photo-ionized at 5 musec delay is approximately equal to the number of exposed residues. The flash photolysis data have been related to inactivation by considering how photolysis of these \"photolabile\" residues can affect enzymic activity, based on the microstructure and available information about permanent alterations and residue specificities. This analysis indicates that hen lysozyme and papain are inactivated by photolysis of an essential Trp residue, that bovine trypsin is inactivated by photolysis of a Trp residue adjacent to the key catalytic Ser and other pathways initiated by excitation of Tyr and Cys, that the efficient photoionization of Tyr and RNase A is not an important inactivating reaction, and that aromatic residues in subtilisn Carlsberg are photosensitive."}
{"id": "PMID:1083838", "title": "Epithelial regeneration of transposed intestine after high doses of X-irradiation.", "content": "The regeneration capacities of normal and transposed small bowel epithelium were compared in rats after applying high doses of X-irradiation. It has been shown that the potency of the mucosa to regenerate is much higher than assumed and that the mucosa can regenerate after single doses varying from 2000-5000 R. Even in the villus epithelium and in flat epithelium covering infiltrates of the lamina propria cells survive, which are still able to resume proliferative activity several days after irradiation.", "contents": "Epithelial regeneration of transposed intestine after high doses of X-irradiation. The regeneration capacities of normal and transposed small bowel epithelium were compared in rats after applying high doses of X-irradiation. It has been shown that the potency of the mucosa to regenerate is much higher than assumed and that the mucosa can regenerate after single doses varying from 2000-5000 R. Even in the villus epithelium and in flat epithelium covering infiltrates of the lamina propria cells survive, which are still able to resume proliferative activity several days after irradiation."}
{"id": "PMID:1083839", "title": "[Effect of gamma rays from 60Co on the in vitro incorporation of palmitic acid in lipids of rat liver microsomes].", "content": "The gamma-rays of 60Co cause a diminution in the incorporation of palmitic acid in the phosphatidic acids and the neutral fats of rat-liver microsomes. This diminution occurs without morphological change in the microsomal membranes, where the enzymes responsible for this incorporation are situated. Some evidence is offered that lipid peroxides may be involved in this inhibition.", "contents": "[Effect of gamma rays from 60Co on the in vitro incorporation of palmitic acid in lipids of rat liver microsomes]. The gamma-rays of 60Co cause a diminution in the incorporation of palmitic acid in the phosphatidic acids and the neutral fats of rat-liver microsomes. This diminution occurs without morphological change in the microsomal membranes, where the enzymes responsible for this incorporation are situated. Some evidence is offered that lipid peroxides may be involved in this inhibition."}
{"id": "PMID:1083840", "title": "Binding of radiation-induced phenylalanine radicals to DNA: influence on the biological activity of the DNA and on its sensitivity to the induction of breaks by gamma-rays.", "content": "When an aqueous solution of double-stranded DNA of bacteriophage PM2 containing phenylalanine and saturated with N2O is irradiated with gamma-rays, radiation-induced phenylalanine radicals are bound covalently. Under the conditions used, about 25 phenylalanine molecules may be bound per lethal hit. For single-stranded PM2 DNA, most of the phenylalanine radicals bound are non-lethal. Evidence is presented that, in double-stranded DNA, an appreciable fraction of the single-strand breaks is induced by phenylalanine radicals. Radiation products of phenylalanine and the phenylalanine bound to the DNA decrease the sensitivity of the DNA to the induction of single-strand breaks. There are indications that the high efficiency of protection by radiation products of phenylalanine is due to their positive charge, which will result in a relatively high concentrations fo these compounds in the vicinity of the negatively-charged DNA molecules.", "contents": "Binding of radiation-induced phenylalanine radicals to DNA: influence on the biological activity of the DNA and on its sensitivity to the induction of breaks by gamma-rays. When an aqueous solution of double-stranded DNA of bacteriophage PM2 containing phenylalanine and saturated with N2O is irradiated with gamma-rays, radiation-induced phenylalanine radicals are bound covalently. Under the conditions used, about 25 phenylalanine molecules may be bound per lethal hit. For single-stranded PM2 DNA, most of the phenylalanine radicals bound are non-lethal. Evidence is presented that, in double-stranded DNA, an appreciable fraction of the single-strand breaks is induced by phenylalanine radicals. Radiation products of phenylalanine and the phenylalanine bound to the DNA decrease the sensitivity of the DNA to the induction of single-strand breaks. There are indications that the high efficiency of protection by radiation products of phenylalanine is due to their positive charge, which will result in a relatively high concentrations fo these compounds in the vicinity of the negatively-charged DNA molecules."}
{"id": "PMID:1083841", "title": "The r.b.e. of different energy neutrons as measured by the heamatopoietic spleen-colony technique.", "content": "The spleen-colony technique has been used for determining the relative biological effectiveness (r.b.e.) for several energies of neutron radiation. Donor mice were exposed for fission and accelerator-generated neutrons at a variety of doses and energies. Immediately after exposure, donor bone-marrow was removed from the hind legs, and standard amounts were injected intravenously into lethally X-irradiated recipients. After 7 days the recipients spleens were evaluated for surface colonies. Dose-response curves were obtained for each type of radiation and the Do was determined. The neutron r.b.e. values from the Do compared with 250kVp X-rays were: reactor 1.58, 252Cf 1:59, and accelerator varied from 2.85 at 1.0 Mev to 0.85 at 13.4 MeV.", "contents": "The r.b.e. of different energy neutrons as measured by the heamatopoietic spleen-colony technique. The spleen-colony technique has been used for determining the relative biological effectiveness (r.b.e.) for several energies of neutron radiation. Donor mice were exposed for fission and accelerator-generated neutrons at a variety of doses and energies. Immediately after exposure, donor bone-marrow was removed from the hind legs, and standard amounts were injected intravenously into lethally X-irradiated recipients. After 7 days the recipients spleens were evaluated for surface colonies. Dose-response curves were obtained for each type of radiation and the Do was determined. The neutron r.b.e. values from the Do compared with 250kVp X-rays were: reactor 1.58, 252Cf 1:59, and accelerator varied from 2.85 at 1.0 Mev to 0.85 at 13.4 MeV."}
{"id": "PMID:1083843", "title": "Enzymatic detection of x-ray-induced strand breaks in nuclear DNA on sections of mouse tissue.", "content": "An enzyme-histochemical method was used to detect X-ray-induced strand breaks in the DNA of mammalian tissue cells. Paraffin sections of ethanol-fixed mouse brain and liver were incubated with three kinds of exogenous DNA polymerizing enzymes, and the amount of in situ incorporation of 3H-deoxyribonucleotides into nuclear DNA was examined by autoradiography. No increase in labelling intensity was observed over nuclei of neurons and astrocytes in cerebral cortex, or over hepatocytes in liver immediately after X-irradiation when compared with unirradiated specimens. In liver Kupffer cells, heavily-labelled nuclei appeared from 30 min to 6 hours after, but were not observed immediately after X-irradiation. This method cannot, therefore, be applied to detect the strand breaks directly induced by X-rays, but it is useful in detecting secondary DNA degradation occurring as a result of nuclear degradation.", "contents": "Enzymatic detection of x-ray-induced strand breaks in nuclear DNA on sections of mouse tissue. An enzyme-histochemical method was used to detect X-ray-induced strand breaks in the DNA of mammalian tissue cells. Paraffin sections of ethanol-fixed mouse brain and liver were incubated with three kinds of exogenous DNA polymerizing enzymes, and the amount of in situ incorporation of 3H-deoxyribonucleotides into nuclear DNA was examined by autoradiography. No increase in labelling intensity was observed over nuclei of neurons and astrocytes in cerebral cortex, or over hepatocytes in liver immediately after X-irradiation when compared with unirradiated specimens. In liver Kupffer cells, heavily-labelled nuclei appeared from 30 min to 6 hours after, but were not observed immediately after X-irradiation. This method cannot, therefore, be applied to detect the strand breaks directly induced by X-rays, but it is useful in detecting secondary DNA degradation occurring as a result of nuclear degradation."}
{"id": "PMID:1083844", "title": "Kinetics of nitroxyl radical reactions. A pulse-radiolysis conductivity study.", "content": "Absolute rate-constants for the reaction of the nitroxyl free radicals TAN and TMPN with radiation-chemically-formed radicals and ions have been determined. k(TAN + X) (in M(-1) sec(-1)=4-0 X 10(9) (for X = OH-), 2-9 X 10(10) (eaq-), 8-0 X 10(9) (H-), 7-2 X 10(8) (-CH2OH), 4-0 X 10(8) (CH3CHOH), 4-3 X 10(8) ((CH3)2COH) 2-8 X 10(8) (-CH2(CH3)2COH), 5-9 X 10(7) (glucose radical), 4-0 X 10(8) (c-C5H9-), and k(TMPN + X)=3-4 X 10(9) (OH-), 7-8 X 10(9) (eq-), 4-9 X 10(9) (H-), 4-4 X 10(8) (-CH2OH), 4-9 X 10(8) (CH3CHOH), 3-6 X 10(8) ((CH3)2COH), 1-5 X 10(8) (-CH2(CH3)2COH), 4-9 X 10(7) (glucose radical), 4-3 X 10(8) (c-C5H9-). Direct measurements by means of a pulse-radiolysis conductivity technique were based on the formation and destruction of charged species in these reactions within certain pH ranges. It is indicated that the radiosensitizing nitroxyls undergo both redox and addition reactions.", "contents": "Kinetics of nitroxyl radical reactions. A pulse-radiolysis conductivity study. Absolute rate-constants for the reaction of the nitroxyl free radicals TAN and TMPN with radiation-chemically-formed radicals and ions have been determined. k(TAN + X) (in M(-1) sec(-1)=4-0 X 10(9) (for X = OH-), 2-9 X 10(10) (eaq-), 8-0 X 10(9) (H-), 7-2 X 10(8) (-CH2OH), 4-0 X 10(8) (CH3CHOH), 4-3 X 10(8) ((CH3)2COH) 2-8 X 10(8) (-CH2(CH3)2COH), 5-9 X 10(7) (glucose radical), 4-0 X 10(8) (c-C5H9-), and k(TMPN + X)=3-4 X 10(9) (OH-), 7-8 X 10(9) (eq-), 4-9 X 10(9) (H-), 4-4 X 10(8) (-CH2OH), 4-9 X 10(8) (CH3CHOH), 3-6 X 10(8) ((CH3)2COH), 1-5 X 10(8) (-CH2(CH3)2COH), 4-9 X 10(7) (glucose radical), 4-3 X 10(8) (c-C5H9-). Direct measurements by means of a pulse-radiolysis conductivity technique were based on the formation and destruction of charged species in these reactions within certain pH ranges. It is indicated that the radiosensitizing nitroxyls undergo both redox and addition reactions."}
{"id": "PMID:1083845", "title": "The effect of repeated small doses of radiation on recovery from sub-lethal damage by Chinese hamster cells irradiated in the plateau phase of growth.", "content": "Unfed plateau-phase cells have been irradiated with either single doses or up to ten fractions of X-rays 6 hours apart. The single-dose survival curve had an extrapolation number of 11-4, and the oxygen-enhancement ratio (o.e.r.) was 3-1. Cells were exposed to multiple fractions of 200 rad or 150 rad in air and 600 rad or 450 rad in hypoxia. The resulting survival curves did not fit a multi-target, single-hit model of cell survival, being much steeper than that would predict. The curves were exponential up to five fractions of X-rays, but tended to bend downwards with increasing number of fractions. Cells that had survived five fractions of 200 rad (or 600 rad in hypoxia) 6 hours apart, were less able to absorb damage as sub-lethal than those which had not previously been exposed to radiation. The ratio of the initial slopes of the fractionated survival curves for irradiation in air and hypoxia was 2-1, implying that the o.e.r. \"on the shoulder\" may be less than that in the exponential region of survival.", "contents": "The effect of repeated small doses of radiation on recovery from sub-lethal damage by Chinese hamster cells irradiated in the plateau phase of growth. Unfed plateau-phase cells have been irradiated with either single doses or up to ten fractions of X-rays 6 hours apart. The single-dose survival curve had an extrapolation number of 11-4, and the oxygen-enhancement ratio (o.e.r.) was 3-1. Cells were exposed to multiple fractions of 200 rad or 150 rad in air and 600 rad or 450 rad in hypoxia. The resulting survival curves did not fit a multi-target, single-hit model of cell survival, being much steeper than that would predict. The curves were exponential up to five fractions of X-rays, but tended to bend downwards with increasing number of fractions. Cells that had survived five fractions of 200 rad (or 600 rad in hypoxia) 6 hours apart, were less able to absorb damage as sub-lethal than those which had not previously been exposed to radiation. The ratio of the initial slopes of the fractionated survival curves for irradiation in air and hypoxia was 2-1, implying that the o.e.r. \"on the shoulder\" may be less than that in the exponential region of survival."}
{"id": "PMID:1083846", "title": "Effects of 60Co irradiation on unfed adults and engorged females of the tick Hyalomma anatolicum.", "content": "Unfed and engorged adults of the cattle tick Hyalomma anatolicum, known to be biological vector of Theileria anmulata in India, were exposed to various levels of gamma-radiation from 0-60 000 R, and the effect on engorgement and redproduction was observed. The radiation tolerance limit for unfed adults is 1000 R for engorgement and reproduction. The sterilizing dose for most of the males is 2000 R but the females can tolerate a slightly higher dose. Engorged females have a tolerance limit of 10 000 R for oviposition and 1000 R for larval development. Higher dosages (20 000-60 000 R) have adverse effects on development, completely inhibiting egg-laying at 60 000 R, but these females (60 000 R) live longer (10 weeks) than those irradiated at lower levels.", "contents": "Effects of 60Co irradiation on unfed adults and engorged females of the tick Hyalomma anatolicum. Unfed and engorged adults of the cattle tick Hyalomma anatolicum, known to be biological vector of Theileria anmulata in India, were exposed to various levels of gamma-radiation from 0-60 000 R, and the effect on engorgement and redproduction was observed. The radiation tolerance limit for unfed adults is 1000 R for engorgement and reproduction. The sterilizing dose for most of the males is 2000 R but the females can tolerate a slightly higher dose. Engorged females have a tolerance limit of 10 000 R for oviposition and 1000 R for larval development. Higher dosages (20 000-60 000 R) have adverse effects on development, completely inhibiting egg-laying at 60 000 R, but these females (60 000 R) live longer (10 weeks) than those irradiated at lower levels."}
{"id": "PMID:1083847", "title": "Phosphate ester cleavage in ribose-5-phosphate induced by OH radicals in deoxygenated aqueous solution. The effect of Fe(II) and Fe(III) ions.", "content": "The reaction of OH radicals and H atoms with ribose-5-phosphate (10(-2) M) in deoxygenated aqueous solution at room temperature (dose-rate 2-1 X 10(17) eV/ml-min, dose 5 X 10(18)-15 X 10(18) eV/ml) leads to the following dephosphorylation products (G-values): ribo-pentodialdose 1 (0-2), 2-hydroxy-4-oxoglutaraldehyde 2 (0-06), 5-deoxy-erythro-pentos-4-ulose 3 (0-1) and 3-oxoglutaraldehyde 4 (0-06). In addition, some minor phosphate free products (total G=0-09) are formed. G(inorganic phosphate) =1-3 and G(H2O2)=0-3. On the addition of 10(-3) M (Fe(III) ions, G (1) and G (3) increase to 0-6 and 0-4 respectively. In the presence of 10(-3) M Fe(II), G(1) and G(3) change to 0-4 and 0-8, respectively. The other dephosphorylation products are suppressed by the iron ions. G(1) also increases on the addition of increasing amounts of H2O2. Each product can be assigned a precursor radical formed by hydrogen abstraction from C-5, C-4 or C-3 of the ribose-5-phosphate molecule. Products 1 and 2 are formed by oxydative dephosphorylation of an alpha-phospho radical with preceeding H2O elimination for product 2. Elimination of H3PO4 from a beta-phospho radical leads to product 3; product 4 is formed by elimination of two molecules of H2O from its precursor radical and hydrolytic cleavage of an enol phosphate bond. Deuterium-labelling experiments and the effects of the iron ions and of H2O2 support the mechanisms proposed. The importance of the dephosphorylation mechanisms for the formation of strand breaks in DNA is discussed with special reference to the effects of the radiosensitizers.", "contents": "Phosphate ester cleavage in ribose-5-phosphate induced by OH radicals in deoxygenated aqueous solution. The effect of Fe(II) and Fe(III) ions. The reaction of OH radicals and H atoms with ribose-5-phosphate (10(-2) M) in deoxygenated aqueous solution at room temperature (dose-rate 2-1 X 10(17) eV/ml-min, dose 5 X 10(18)-15 X 10(18) eV/ml) leads to the following dephosphorylation products (G-values): ribo-pentodialdose 1 (0-2), 2-hydroxy-4-oxoglutaraldehyde 2 (0-06), 5-deoxy-erythro-pentos-4-ulose 3 (0-1) and 3-oxoglutaraldehyde 4 (0-06). In addition, some minor phosphate free products (total G=0-09) are formed. G(inorganic phosphate) =1-3 and G(H2O2)=0-3. On the addition of 10(-3) M (Fe(III) ions, G (1) and G (3) increase to 0-6 and 0-4 respectively. In the presence of 10(-3) M Fe(II), G(1) and G(3) change to 0-4 and 0-8, respectively. The other dephosphorylation products are suppressed by the iron ions. G(1) also increases on the addition of increasing amounts of H2O2. Each product can be assigned a precursor radical formed by hydrogen abstraction from C-5, C-4 or C-3 of the ribose-5-phosphate molecule. Products 1 and 2 are formed by oxydative dephosphorylation of an alpha-phospho radical with preceeding H2O elimination for product 2. Elimination of H3PO4 from a beta-phospho radical leads to product 3; product 4 is formed by elimination of two molecules of H2O from its precursor radical and hydrolytic cleavage of an enol phosphate bond. Deuterium-labelling experiments and the effects of the iron ions and of H2O2 support the mechanisms proposed. The importance of the dephosphorylation mechanisms for the formation of strand breaks in DNA is discussed with special reference to the effects of the radiosensitizers."}
{"id": "PMID:1083848", "title": "Negative pion irradiation of mammalian cells. 1. Survival characteristics of monolayers and spheroids of Chinese hamster lung cells.", "content": "Monolayers and spheroids of Chinese hamster cells (V79) were subjected to negative pion irradiation under aerobic conditions. R.b.e. values in the pion peak of 1-8 and 1-5 were obtained for monolayers and spheroids, respectively, whereas the r.b.e. for the plateau was found to be slightly higher than 1. In addition, it was observed that the higher resistance of the V79 spheroid cells than the monolayers to gamma-irradiation is not diminished in the pion peak, suggesting that the underlying phenomenon of intercellular communication influences cell survival even after high-LET irradiation.", "contents": "Negative pion irradiation of mammalian cells. 1. Survival characteristics of monolayers and spheroids of Chinese hamster lung cells. Monolayers and spheroids of Chinese hamster cells (V79) were subjected to negative pion irradiation under aerobic conditions. R.b.e. values in the pion peak of 1-8 and 1-5 were obtained for monolayers and spheroids, respectively, whereas the r.b.e. for the plateau was found to be slightly higher than 1. In addition, it was observed that the higher resistance of the V79 spheroid cells than the monolayers to gamma-irradiation is not diminished in the pion peak, suggesting that the underlying phenomenon of intercellular communication influences cell survival even after high-LET irradiation."}
{"id": "PMID:1083853", "title": "Cirrhosis and heterozygous alpha1-antitrypsin deficiency in a 4-year-old girl.", "content": "A 4-year-old girl with hepatic cirrhosis was found to have alpha1-antitrypsin deficiency. The method of FAGERHOL detected that she is a heterozygous carrier of the Pi SZ type. Investigations with the light and the electron microscope and immunofluorescence tests confirmed the diagnosis. The values of alpha1-antitrypsin and trypsin inhibitory capacity gave intermediate values. The fact that in the patient, the Z allele was combined with an S allele may have contributed to the severity of the illness.", "contents": "Cirrhosis and heterozygous alpha1-antitrypsin deficiency in a 4-year-old girl. A 4-year-old girl with hepatic cirrhosis was found to have alpha1-antitrypsin deficiency. The method of FAGERHOL detected that she is a heterozygous carrier of the Pi SZ type. Investigations with the light and the electron microscope and immunofluorescence tests confirmed the diagnosis. The values of alpha1-antitrypsin and trypsin inhibitory capacity gave intermediate values. The fact that in the patient, the Z allele was combined with an S allele may have contributed to the severity of the illness."}
{"id": "PMID:1083854", "title": "[Facial paresis following stapedectomy (author's transl)].", "content": "An ususual complication after stapedectomy for otosclerosis is reported. After two vain attempts elsewhere, the tympanic cavity was opened for the third time. Since the incus was missing, a malleovestibuloplasty was undertaken, using the Schuknecht wire prosthesis. Hearing was very good. One day after the operation, a facial paralysis developed, and increased over the following days. After 18 days, the nerve  was found to be oedematous and swollen above the fenestra ovale upto the level of the promontory and had forced the prosthesis out of the fenestra ovale. Six weeks later, nerve function was normal.", "contents": "[Facial paresis following stapedectomy (author's transl)]. An ususual complication after stapedectomy for otosclerosis is reported. After two vain attempts elsewhere, the tympanic cavity was opened for the third time. Since the incus was missing, a malleovestibuloplasty was undertaken, using the Schuknecht wire prosthesis. Hearing was very good. One day after the operation, a facial paralysis developed, and increased over the following days. After 18 days, the nerve  was found to be oedematous and swollen above the fenestra ovale upto the level of the promontory and had forced the prosthesis out of the fenestra ovale. Six weeks later, nerve function was normal."}
{"id": "PMID:1083855", "title": "Hormonal regulation of gap junction differentiation.", "content": "Thin-section, tracer, and freeze-cleave experiments on hypophysectomized Rana pipiens larvae reveal that gap junctions form between differentiating ependymoglial cells in response to thyroid hormone. These junctions assemble in large particle-free areas of the plasma membrane known as formation plaques. Between 20 and 40 h after hormone application, formation plaque area increases approximately 26-fold while gap junction area rises about 20-fold. The differentiation of these junctions requires the synthesis of new protein and probably RNA as well. On the basis of inhibitor experiments, it can be reported that formation plaques develop at about 16-20 h after hormone treatment and stages in the construction of gap junctions appear 4-8 h later. These studies suggest that gap junction subunits are synthesized and inserted into formation plaque membrane during the differentiation of the anuran ependymoglial cells.", "contents": "Hormonal regulation of gap junction differentiation. Thin-section, tracer, and freeze-cleave experiments on hypophysectomized Rana pipiens larvae reveal that gap junctions form between differentiating ependymoglial cells in response to thyroid hormone. These junctions assemble in large particle-free areas of the plasma membrane known as formation plaques. Between 20 and 40 h after hormone application, formation plaque area increases approximately 26-fold while gap junction area rises about 20-fold. The differentiation of these junctions requires the synthesis of new protein and probably RNA as well. On the basis of inhibitor experiments, it can be reported that formation plaques develop at about 16-20 h after hormone treatment and stages in the construction of gap junctions appear 4-8 h later. These studies suggest that gap junction subunits are synthesized and inserted into formation plaque membrane during the differentiation of the anuran ependymoglial cells."}
{"id": "PMID:1083857", "title": "Topological analysis of the brain stem of the frogs Rana esculenta and Rana catesbeiana.", "content": "The ventricular sulcal pattern and the cytoarchitectonic organization of the brain stem of the frogs Rana esculenta and Rana catesbeiana have been studied in transversely cut, Nissl stained serial sections. Four longitudinal sulci, the sulcus medianus inferior, the sulcus intermedius ventralis, the sulcus limitans and the sulcus medianus superior could be distinguished in both species. A fifth longitudinal groove, the sulcus intermedius dorsalis, was found only in Rana esculenta. With the aid of the usual cytoarchitectonic criteria 25 cell masses have been delineated in Rana esculenta and 27 in Rana catesbeiana. These cell masses can be distributed over the following categories (numbers added in brackets for Rana catesbeiana, if different from those in Rana esculenta): primary efferent or motor, 8; primary afferent or sensory, 4(6); \"relay\" centers, 7. Contrary to statements in the literature the reticular formation can be divided into six separate cell groups. The majority of the nuclei form part of the central gray, which constitutes a rather wide zone in anurans; three reticular nuclei lie partly within the stratum griseum and partly within the stratum album; six nuclei are entirely embedded in the stratum album. The morphological pattern of the cell masses and their relationship to the ventricular sulci were studied with the aid of a graphical reconstruction procedure termed topological analysis (cf. Nieuwenhuys, '74 and figs. 15, 16). This analysis yielded the following results: The sulcus limitans extends throughout the rhombencephalon, dividing this brain part into a basal plate and an alar plate. The cell masses in the basal plate fit into two longitudinal zones, a medial area ventralis and a lateral area intermedioventralis. The area ventralis contains three somatic motor nuclei (IV, VI and XII) and the rhombencephalic medial reticular zone. The latter may be primarily considered as a somatic motor coordinating center. The area intermedioventralis contains the visceral motor nuclei of V, VII, IX and X. However, the basal plate also contains a number of non-motor centers, for example the superior olive. The alar plate contains visceral sensory, general somatic sensory and special somatic sensory centers. Two cell masses, the nucleusfasciculi solitarii and the nucleus visceralis secundarius, represent together a discontinuous visceral sensory zone. Both of these nuclei are situated immediately dorsal to the sulcus limitans. The special somatic sensory area, i.e., the area of termination of the eighth nerve, occupies a considerable part of the alar plate. This area comprises, apart from a large zone of diffuse gray, three distinct cell masses...", "contents": "Topological analysis of the brain stem of the frogs Rana esculenta and Rana catesbeiana. The ventricular sulcal pattern and the cytoarchitectonic organization of the brain stem of the frogs Rana esculenta and Rana catesbeiana have been studied in transversely cut, Nissl stained serial sections. Four longitudinal sulci, the sulcus medianus inferior, the sulcus intermedius ventralis, the sulcus limitans and the sulcus medianus superior could be distinguished in both species. A fifth longitudinal groove, the sulcus intermedius dorsalis, was found only in Rana esculenta. With the aid of the usual cytoarchitectonic criteria 25 cell masses have been delineated in Rana esculenta and 27 in Rana catesbeiana. These cell masses can be distributed over the following categories (numbers added in brackets for Rana catesbeiana, if different from those in Rana esculenta): primary efferent or motor, 8; primary afferent or sensory, 4(6); \"relay\" centers, 7. Contrary to statements in the literature the reticular formation can be divided into six separate cell groups. The majority of the nuclei form part of the central gray, which constitutes a rather wide zone in anurans; three reticular nuclei lie partly within the stratum griseum and partly within the stratum album; six nuclei are entirely embedded in the stratum album. The morphological pattern of the cell masses and their relationship to the ventricular sulci were studied with the aid of a graphical reconstruction procedure termed topological analysis (cf. Nieuwenhuys, '74 and figs. 15, 16). This analysis yielded the following results: The sulcus limitans extends throughout the rhombencephalon, dividing this brain part into a basal plate and an alar plate. The cell masses in the basal plate fit into two longitudinal zones, a medial area ventralis and a lateral area intermedioventralis. The area ventralis contains three somatic motor nuclei (IV, VI and XII) and the rhombencephalic medial reticular zone. The latter may be primarily considered as a somatic motor coordinating center. The area intermedioventralis contains the visceral motor nuclei of V, VII, IX and X. However, the basal plate also contains a number of non-motor centers, for example the superior olive. The alar plate contains visceral sensory, general somatic sensory and special somatic sensory centers. Two cell masses, the nucleusfasciculi solitarii and the nucleus visceralis secundarius, represent together a discontinuous visceral sensory zone. Both of these nuclei are situated immediately dorsal to the sulcus limitans. The special somatic sensory area, i.e., the area of termination of the eighth nerve, occupies a considerable part of the alar plate. This area comprises, apart from a large zone of diffuse gray, three distinct cell masses..."}
{"id": "PMID:1083858", "title": "alpha1-Antitrypsin deficiency and skin abnormalities.", "content": "A 19-year-old Moroccan male was found to have total absence of serum alpha1-antitrypsin, a major inhibitor of elastase. This patient had chronic obstructive lung disease, hyperextensibility of the skin over the cheeks and wrists, and hyperlaxity of the hand joints. Microscopic sections of the skin revealed a thickened dermis with shortened and rarefied elastic fibers. Ultrastructural study showed collagen fibers with variable and irregular diameters. Elastic fibers were scarce and their relatively poor matrix was surrounded by numberous microfibrils. The outline of the fibers was irregular with deep recesses filled with microfibrils. The ergastoplasm of the fibroblasts was well developed. The differential diagnosis with other connective dystrophies showed the original characteristic of this case. Clinically and histopathologically, the skin abnormalities are probably related to the deficiency in elastase inhibitor.", "contents": "alpha1-Antitrypsin deficiency and skin abnormalities. A 19-year-old Moroccan male was found to have total absence of serum alpha1-antitrypsin, a major inhibitor of elastase. This patient had chronic obstructive lung disease, hyperextensibility of the skin over the cheeks and wrists, and hyperlaxity of the hand joints. Microscopic sections of the skin revealed a thickened dermis with shortened and rarefied elastic fibers. Ultrastructural study showed collagen fibers with variable and irregular diameters. Elastic fibers were scarce and their relatively poor matrix was surrounded by numberous microfibrils. The outline of the fibers was irregular with deep recesses filled with microfibrils. The ergastoplasm of the fibroblasts was well developed. The differential diagnosis with other connective dystrophies showed the original characteristic of this case. Clinically and histopathologically, the skin abnormalities are probably related to the deficiency in elastase inhibitor."}
{"id": "PMID:1083859", "title": "Preliminary cutaneous pathology observations with the freeze-fracture technique.", "content": "The freeze-fracture technique was used to study acantholytic cells of untreated pemphigus patients and the cells of histocytosis X. On the membranes of acanthocytosis cells, the particles are randomly distributed and lack the typical desmosome organization, while tight and gap junctions are still found. The study of the cells of histocytosis X provided a better understanding of the structure of the Langerhans granule. The paracrystalline organization of the inner face of the disc-shaped portion does not seem to involve the membranes limiting the granule, but rather to be confined to a periodically arranged, possibly proteic substance contained between them.", "contents": "Preliminary cutaneous pathology observations with the freeze-fracture technique. The freeze-fracture technique was used to study acantholytic cells of untreated pemphigus patients and the cells of histocytosis X. On the membranes of acanthocytosis cells, the particles are randomly distributed and lack the typical desmosome organization, while tight and gap junctions are still found. The study of the cells of histocytosis X provided a better understanding of the structure of the Langerhans granule. The paracrystalline organization of the inner face of the disc-shaped portion does not seem to involve the membranes limiting the granule, but rather to be confined to a periodically arranged, possibly proteic substance contained between them."}
{"id": "PMID:1083861", "title": "Immunobiology of aging.", "content": "Normal immune functions can begin to decline shortly after an individual reaches sexual maturity. Although changes in cellular environment are partially responsible, the decline is due primarily to changes within the cells, especially the T cells and to some extent the stem cells. This is reflected in their inability to proliferate and differentiate efficiently. What needs to be resolved is whether the altered properties of T cells and stem cells are permanent or reversible and, if permanent, whether they are due to a stochastic or genetically programmed event. The decline with age in certain normal immune functions is associated with an increase in the frequency of autoimmune and immune complex diseases, certain types of cancer, and viral and fungal infections. These diseases, compromise normal immune functions in short-lived strains of mice. In long-lived mice and in humans, however, the decline in immune functions to threshold levels seems to predispose in individuals to illness.", "contents": "Immunobiology of aging. Normal immune functions can begin to decline shortly after an individual reaches sexual maturity. Although changes in cellular environment are partially responsible, the decline is due primarily to changes within the cells, especially the T cells and to some extent the stem cells. This is reflected in their inability to proliferate and differentiate efficiently. What needs to be resolved is whether the altered properties of T cells and stem cells are permanent or reversible and, if permanent, whether they are due to a stochastic or genetically programmed event. The decline with age in certain normal immune functions is associated with an increase in the frequency of autoimmune and immune complex diseases, certain types of cancer, and viral and fungal infections. These diseases, compromise normal immune functions in short-lived strains of mice. In long-lived mice and in humans, however, the decline in immune functions to threshold levels seems to predispose in individuals to illness."}
{"id": "PMID:1083862", "title": "Autoimmune disease: the consequence of deficient T-cell function?", "content": "The age-related decrease in T-lymphocyte function is discussed in relation to the onset of autoimmune disease. It is postulated that the decrease in T-cell \"immune surveillance\" permits: a) the ingress of viruses whose enzymes modify host glycoproteins and render them immunogenic, and b) the replication of viruses incorporated into the genome of cells during infections in early life. The existence of immunodeficiencies too subtle to be detected by our present laboratory methods is postulated and discussed in relation to diseases observed in geriatric patients. It would seem that the prevention of some geriatric diseases should begin in the pediatrician's office.", "contents": "Autoimmune disease: the consequence of deficient T-cell function? The age-related decrease in T-lymphocyte function is discussed in relation to the onset of autoimmune disease. It is postulated that the decrease in T-cell \"immune surveillance\" permits: a) the ingress of viruses whose enzymes modify host glycoproteins and render them immunogenic, and b) the replication of viruses incorporated into the genome of cells during infections in early life. The existence of immunodeficiencies too subtle to be detected by our present laboratory methods is postulated and discussed in relation to diseases observed in geriatric patients. It would seem that the prevention of some geriatric diseases should begin in the pediatrician's office."}
{"id": "PMID:1083863", "title": "Tumor immunology, autoimmunity and aging.", "content": "Cell-mediated immune function declines with aging, and may be associated with autoimmunity and malignancy. Humoral immune responses also decline with aging. The chief age-related effect on the immune system is a decrease in T-cell function. The \"thymus clock\" and immunogenesis are discussed in relation to aging. In animals, attempts at immunologic rejuvenation by cellular or hormonal means have not been successful as the results attained by genetic manipulation.", "contents": "Tumor immunology, autoimmunity and aging. Cell-mediated immune function declines with aging, and may be associated with autoimmunity and malignancy. Humoral immune responses also decline with aging. The chief age-related effect on the immune system is a decrease in T-cell function. The \"thymus clock\" and immunogenesis are discussed in relation to aging. In animals, attempts at immunologic rejuvenation by cellular or hormonal means have not been successful as the results attained by genetic manipulation."}
{"id": "PMID:1083864", "title": "Results of open heart surgery in the septuagenarian.", "content": "Open heart surgery has been extended to the septuagenarian. At the Long Island Jewish-Hillside Medical Center, 42 patients 70 to 79 years old underwent 43 open heart procedures in the past 7 years. Their average age was 73 years and 62% were male. Eighteen underwent aortic valve replacement; 7 had mitral valve replacement; 18 had coronary bypasses (3 with valve replacement); exploration for pulmonary embolism was done in 1; 1 patient had tricuspid replacement with mitral commissurotomy; 17% (7/42) died during hospitalization or within 30 days of surgery. Mean follow-up is 25 mo., and an additional 19% (8/42) have died at an average of 27 mo. after surgery. Open heart surgery is feasible for the elderly, but at an increased risk when compared to the younger patient submitted to the same procedure.", "contents": "Results of open heart surgery in the septuagenarian. Open heart surgery has been extended to the septuagenarian. At the Long Island Jewish-Hillside Medical Center, 42 patients 70 to 79 years old underwent 43 open heart procedures in the past 7 years. Their average age was 73 years and 62% were male. Eighteen underwent aortic valve replacement; 7 had mitral valve replacement; 18 had coronary bypasses (3 with valve replacement); exploration for pulmonary embolism was done in 1; 1 patient had tricuspid replacement with mitral commissurotomy; 17% (7/42) died during hospitalization or within 30 days of surgery. Mean follow-up is 25 mo., and an additional 19% (8/42) have died at an average of 27 mo. after surgery. Open heart surgery is feasible for the elderly, but at an increased risk when compared to the younger patient submitted to the same procedure."}
{"id": "PMID:1083866", "title": "IgM complex receptors on subpopulations of murine lymphocytes.", "content": "Murine lymphocytes from spleen, lymph node, and thymus were examined for IgM complex receptors. Lymphocytes from all three organs were found to bind SRBC sensitized with IgM from various sources including: primary anti-SRBC serum, murine and rabbit anti-Escherichia coli LPS sera, and a murine IgM myeloma (MOPC 104E). Rosette formation by lymphocytes with IgM-sensitized SRBC was inhibited by soluble antigen-IgM complexes but not by IgM or antigen alone. Rosette formation was also inhibited by human IgM (Fc)5mu but not by Fab mu. Antiserum and complement treatment of the cells and subsequent recovery of the viable cells by trypsinization, filtration, and washing revealed the IgM rosette-forming cell (RFC) in the thymus to be a T cell. Spleen on the other hand was found to contain both B and T cells capable of binding IgM sensitized SRBC. Removal of both B and T cells from spleen cell suspensions eliminated all IgM RFC. The IgM complex receptor was found to be trypsin insensitive. Anti-Ig column fractionation enriched IgM RFC in spleen and lymph node suspensions passed through the columns, whereas cells bearing surface Ig, IgG complex receptors, and C3 receptors were retained in the columns.", "contents": "IgM complex receptors on subpopulations of murine lymphocytes. Murine lymphocytes from spleen, lymph node, and thymus were examined for IgM complex receptors. Lymphocytes from all three organs were found to bind SRBC sensitized with IgM from various sources including: primary anti-SRBC serum, murine and rabbit anti-Escherichia coli LPS sera, and a murine IgM myeloma (MOPC 104E). Rosette formation by lymphocytes with IgM-sensitized SRBC was inhibited by soluble antigen-IgM complexes but not by IgM or antigen alone. Rosette formation was also inhibited by human IgM (Fc)5mu but not by Fab mu. Antiserum and complement treatment of the cells and subsequent recovery of the viable cells by trypsinization, filtration, and washing revealed the IgM rosette-forming cell (RFC) in the thymus to be a T cell. Spleen on the other hand was found to contain both B and T cells capable of binding IgM sensitized SRBC. Removal of both B and T cells from spleen cell suspensions eliminated all IgM RFC. The IgM complex receptor was found to be trypsin insensitive. Anti-Ig column fractionation enriched IgM RFC in spleen and lymph node suspensions passed through the columns, whereas cells bearing surface Ig, IgG complex receptors, and C3 receptors were retained in the columns."}
{"id": "PMID:1083867", "title": "Splenic suppressing factor: purification and characterization of a factor suppressing thymidine incorporation into activated lymphocytes.", "content": "Suppressing activity upon the mitogen-activated lymphocytes was found in the supernatant (SUP) from the culture of mouse spleen, high-density subpopulation of thymocytes, and peritoneal exudate cells. Suppressing factor was obtained from the non-stimulated lymphocytes cultured for 24 to 36 hr with or without serum. Suppressing activity in the SUP was observed in the incorporation of 3H-thymidine, 3H-uridine, and 3H-leucine into Con A-activated lymphocytes or in the proliferation of L cells. Suppressing factor partially purified by Sephadex G-25 column chromatography was a heat-stable and dialyzable substance(s). Further purification and isolation of this factor by two-dimensional thin layer chromatography revealed that this was thymidine and thymidine monophosphate. The suppression in 3H-thymidine incorporation was attributed to the dilution effect of cold thymidine released from cultured lymphocytes.", "contents": "Splenic suppressing factor: purification and characterization of a factor suppressing thymidine incorporation into activated lymphocytes. Suppressing activity upon the mitogen-activated lymphocytes was found in the supernatant (SUP) from the culture of mouse spleen, high-density subpopulation of thymocytes, and peritoneal exudate cells. Suppressing factor was obtained from the non-stimulated lymphocytes cultured for 24 to 36 hr with or without serum. Suppressing activity in the SUP was observed in the incorporation of 3H-thymidine, 3H-uridine, and 3H-leucine into Con A-activated lymphocytes or in the proliferation of L cells. Suppressing factor partially purified by Sephadex G-25 column chromatography was a heat-stable and dialyzable substance(s). Further purification and isolation of this factor by two-dimensional thin layer chromatography revealed that this was thymidine and thymidine monophosphate. The suppression in 3H-thymidine incorporation was attributed to the dilution effect of cold thymidine released from cultured lymphocytes."}
{"id": "PMID:1083868", "title": "Reaginic antibody formation in the mouse. VII. Induction of suppressor T cells for IgE and IgG antibody responses.", "content": "Intravenous injections of urea-denatured ovalbumin (UD-OA) into OA-primed high responder mice suppressed the antibody response not only to the priming antigen but also to subsequent immunization with dinitrophenyl derivatives of OA (DNP-OA). The transfer of normal spleen cells or OA-primed spleen cells into UD-OA-treated animals did not restore the capacity of responding to DNP-OA to form anti-DNP IgE and IgG antibodies. The transfer of splenic T cell fraction from the UD-OA-treated animals into normal syngeneic mice diminished both IgE and IgG antibody responses of the recipients to DNP-OA. The B cell-rich fraction from the same donors failed to affect the anti-hapten antibody response and enhanced anti-cancer (OA) IgG antibody response of the recipients. It was also found that the transfer of T cell-rich fraction of OA-primed spleen cells failed to suppress antibody response of the recipients to DNP-OA. The results indicated that spleen cells of UD-OA-treated mice contained suppressor T cells which are distinct from helper cells. Suppressive activity of T cells in the UD-OA treated animals was specific for OA. The transfer of the T cell-rich fraction failed to suppress anti-DNP antibody response of the recipients to DNP-KLH.", "contents": "Reaginic antibody formation in the mouse. VII. Induction of suppressor T cells for IgE and IgG antibody responses. Intravenous injections of urea-denatured ovalbumin (UD-OA) into OA-primed high responder mice suppressed the antibody response not only to the priming antigen but also to subsequent immunization with dinitrophenyl derivatives of OA (DNP-OA). The transfer of normal spleen cells or OA-primed spleen cells into UD-OA-treated animals did not restore the capacity of responding to DNP-OA to form anti-DNP IgE and IgG antibodies. The transfer of splenic T cell fraction from the UD-OA-treated animals into normal syngeneic mice diminished both IgE and IgG antibody responses of the recipients to DNP-OA. The B cell-rich fraction from the same donors failed to affect the anti-hapten antibody response and enhanced anti-cancer (OA) IgG antibody response of the recipients. It was also found that the transfer of T cell-rich fraction of OA-primed spleen cells failed to suppress antibody response of the recipients to DNP-OA. The results indicated that spleen cells of UD-OA-treated mice contained suppressor T cells which are distinct from helper cells. Suppressive activity of T cells in the UD-OA treated animals was specific for OA. The transfer of the T cell-rich fraction failed to suppress anti-DNP antibody response of the recipients to DNP-KLH."}
{"id": "PMID:1083869", "title": "Enumeration and isolation of rabbit T and B lymphocytes by using antibody-coated erythrocytes.", "content": "Rosette formation with antibody-coated erythrocytes (Ab-E) was employed for the enumeration and isolation of rabbit B cells (Ig+T-) and T cells (Ig-T+). The cells bearing surface Ig (Ig+ cells) were enumerated by a direct immunocytoadhesion technique utilizing anti-rabbit IgG antibody-coated erythrocytes (Ab-E). To enumerate cells bearing thymus cell antigen (T+ cells), an indirect rosette technique was used in which lymphocytes were first sensitized with guinea pig anti-rabbit thymus cell antiserum and then rosetted with anti-guinea pig IgG Ab-E. To demonstrate the specificity of the anti-thymus cell antiserum, a 51Cr radioimmunoassay for counting rosettes was employed along with visual counting to enumerate Ig+ and T+ cells in lymph node cell populations. When Ig+ and T+ lymph node cells were rosetted simultaneously with sheep and human erythrocytes, no mixed rosettes (less than 1%) were observed. Ficoll-Hypaque gradient centrifugation was used to obtain purified Ig+T- and Ig-T+ cells by removing rosetted T+ and Ig+ cells, respectively. The purity of isolated Ig-T+ cells was indicated by 94 to 95% indirect rosetting with anti-thymus cell antiserum and by 0 to 3% direct rosetting with anti-rabbit IgG Ab-E. The purity of isolated Ig+T- cells was indicated by 90 t0 94% direct rosetting with anti-rabbit IgG Ab-E and by 2 to 3% indirect rosetting with anti-thymus cell antiserum. The percentage of Ig+T- and Ig-T+ cells were determined in peripheral blood and in various lymphoid organs. The isolated Ig+T- and Ig-T+ cells were also characterized by their responses to mitogens. Thus, nearly pure Ig+T- and Ig-T+ cells were isolated by \"negative selection,\"  which should minimize functional changes of the cells, and thereby facilitate the study of their biologic properties, e.g., their response to mitogens.", "contents": "Enumeration and isolation of rabbit T and B lymphocytes by using antibody-coated erythrocytes. Rosette formation with antibody-coated erythrocytes (Ab-E) was employed for the enumeration and isolation of rabbit B cells (Ig+T-) and T cells (Ig-T+). The cells bearing surface Ig (Ig+ cells) were enumerated by a direct immunocytoadhesion technique utilizing anti-rabbit IgG antibody-coated erythrocytes (Ab-E). To enumerate cells bearing thymus cell antigen (T+ cells), an indirect rosette technique was used in which lymphocytes were first sensitized with guinea pig anti-rabbit thymus cell antiserum and then rosetted with anti-guinea pig IgG Ab-E. To demonstrate the specificity of the anti-thymus cell antiserum, a 51Cr radioimmunoassay for counting rosettes was employed along with visual counting to enumerate Ig+ and T+ cells in lymph node cell populations. When Ig+ and T+ lymph node cells were rosetted simultaneously with sheep and human erythrocytes, no mixed rosettes (less than 1%) were observed. Ficoll-Hypaque gradient centrifugation was used to obtain purified Ig+T- and Ig-T+ cells by removing rosetted T+ and Ig+ cells, respectively. The purity of isolated Ig-T+ cells was indicated by 94 to 95% indirect rosetting with anti-thymus cell antiserum and by 0 to 3% direct rosetting with anti-rabbit IgG Ab-E. The purity of isolated Ig+T- cells was indicated by 90 t0 94% direct rosetting with anti-rabbit IgG Ab-E and by 2 to 3% indirect rosetting with anti-thymus cell antiserum. The percentage of Ig+T- and Ig-T+ cells were determined in peripheral blood and in various lymphoid organs. The isolated Ig+T- and Ig-T+ cells were also characterized by their responses to mitogens. Thus, nearly pure Ig+T- and Ig-T+ cells were isolated by \"negative selection,\"  which should minimize functional changes of the cells, and thereby facilitate the study of their biologic properties, e.g., their response to mitogens."}
{"id": "PMID:1083870", "title": "The suppressive effect of immunization on the proliferative responses of rat T cells in vitro. II. Abrogation of antigen-induced suppression by selective cytotoxic agents.", "content": "Rats given large i.v. doses of ovalbumin or sheep erythrocytes manifest suppressed spleen cell responses (3H-thymidine incorporation) to PHA within hours. Removal of glass wool-adherent cells totally restores responsiveness to that of normal nonadherent spleen cell cultures. Carrageenan, selectively toxic for macrophages, partially restores responses of antigen-suppressed spleen cells in culture, suggesting a supportive role for macrophages in the suppression phenomenon. Treatment of donors with low doses of cyclophosphamide (20 to 50 mg/kg) at the time of antigen injection abrogates the ability of their spleen cells to suppress the responses of normal cells to PHA. The low dose of cyclophosphamide required indicates a target other than the B cell or macrophage and suggests the possibility that cyclophosphamide eliminates the suppressor T cell component of the macrophage-T cell complex.", "contents": "The suppressive effect of immunization on the proliferative responses of rat T cells in vitro. II. Abrogation of antigen-induced suppression by selective cytotoxic agents. Rats given large i.v. doses of ovalbumin or sheep erythrocytes manifest suppressed spleen cell responses (3H-thymidine incorporation) to PHA within hours. Removal of glass wool-adherent cells totally restores responsiveness to that of normal nonadherent spleen cell cultures. Carrageenan, selectively toxic for macrophages, partially restores responses of antigen-suppressed spleen cells in culture, suggesting a supportive role for macrophages in the suppression phenomenon. Treatment of donors with low doses of cyclophosphamide (20 to 50 mg/kg) at the time of antigen injection abrogates the ability of their spleen cells to suppress the responses of normal cells to PHA. The low dose of cyclophosphamide required indicates a target other than the B cell or macrophage and suggests the possibility that cyclophosphamide eliminates the suppressor T cell component of the macrophage-T cell complex."}
{"id": "PMID:1083871", "title": "Differentiation of thymocytes: evidence that induction of the surface phenotype requires transcription and translation.", "content": "Induction of thymocyte differentiation requires transcription of DNA and translation of RNA but not replication of DNA. This was determined in vitro by studying the effects of cytosine arabinoside, hydroxyurea, actinomycin D, camptothecin, cordycepin, cycloheximide, and puromycin on the induction by thymopoietin of thymocyte differentiation, measured by the acquisition of the differentiation allo-antigens TL and Thy-1 by and inducible fraction of mouse spleen cells. This requirement for transcription does not establish whether the new mRNA represents transcripts of structural genes for the thymocyte allo-antigen markers; an answer to this question awaits the results of further experiments.", "contents": "Differentiation of thymocytes: evidence that induction of the surface phenotype requires transcription and translation. Induction of thymocyte differentiation requires transcription of DNA and translation of RNA but not replication of DNA. This was determined in vitro by studying the effects of cytosine arabinoside, hydroxyurea, actinomycin D, camptothecin, cordycepin, cycloheximide, and puromycin on the induction by thymopoietin of thymocyte differentiation, measured by the acquisition of the differentiation allo-antigens TL and Thy-1 by and inducible fraction of mouse spleen cells. This requirement for transcription does not establish whether the new mRNA represents transcripts of structural genes for the thymocyte allo-antigen markers; an answer to this question awaits the results of further experiments."}
{"id": "PMID:1083872", "title": "The significance of varying SRBC/lymphocyte ratio in T cell rosette formation.", "content": "The incubation ratio of sheep red blood cells (SRBC) to lymphocytes is a critical factor in rosette formation, whereas the length of time SRBC and lymphocytes are incubated together does not significantly affect the percentage of lymphocytes forming rosettes. The graph obtained by plotting percentage of rosette formation against the ratio of SRBC to lymphocytes is similar to that resulting from the formation of bimolecular complexes. If rosette formation is analogous to formation of bimolecular complexes, maximal rosette formation occurs when the system is saturated, i.e., with excess SRBC, and is a measure of the total capacity of a lymphocyte population to form rosettes. In addition, the percentage of rosette formation observed at a limiting SRBC/lymphocyte ratio gives an indication of the avidity of the lymphocytes for SRBC. This interpretation may provide an explanation for the difference between the \"active\" and \"total\" rosettes. When the log of the SRBC/lymphocyte ratio is plotted against percentage of rosette formation, a straight line is obtained, suggesting that within a given normal lymphocyte sample, T cell subsets with different avidities are not detected by rosette formation at different SRBC/lymphocyte ratios.", "contents": "The significance of varying SRBC/lymphocyte ratio in T cell rosette formation. The incubation ratio of sheep red blood cells (SRBC) to lymphocytes is a critical factor in rosette formation, whereas the length of time SRBC and lymphocytes are incubated together does not significantly affect the percentage of lymphocytes forming rosettes. The graph obtained by plotting percentage of rosette formation against the ratio of SRBC to lymphocytes is similar to that resulting from the formation of bimolecular complexes. If rosette formation is analogous to formation of bimolecular complexes, maximal rosette formation occurs when the system is saturated, i.e., with excess SRBC, and is a measure of the total capacity of a lymphocyte population to form rosettes. In addition, the percentage of rosette formation observed at a limiting SRBC/lymphocyte ratio gives an indication of the avidity of the lymphocytes for SRBC. This interpretation may provide an explanation for the difference between the \"active\" and \"total\" rosettes. When the log of the SRBC/lymphocyte ratio is plotted against percentage of rosette formation, a straight line is obtained, suggesting that within a given normal lymphocyte sample, T cell subsets with different avidities are not detected by rosette formation at different SRBC/lymphocyte ratios."}
{"id": "PMID:1083873", "title": "Immunoglobulin-positive mononuclear cells in human peripheral blood: detection by mixed anti-globulin and direct anti-globulin-rosetting reactions.", "content": "Ig-bearing mononuclear cells were identified in Ficoll-Hypaque preparations of human peripheral blood by using mixed anti-globulin (MAG) and direct anti-globulin rosettes; indicator cells consisted of sheep erythrocytes coated with human F(ab')2 or anti-F(ab')2 antibody, respectively. Of the cell population isolated from 10 normal subjects, a mean of 68% was lymphocytes. However, fewer than 50% of the cells with detectable surface Ig were lymphocytes. On viable cell preparations using chromic chloride-treated sheep erythrocytes (CrCl3SRBC) coated with anti-F(ab')2 antibody, a mean of 20.1% of the lymphocytes formed rosettes, i.e., were B. Up to 6% of peripheral blood lymphocytes formed mixed Ig-rosettes and E-rosettes. On viable lymphocytes using F(ab')2-coated CrCl3SRBC, MAG rosettes were insensitive in detection of B lymphocytes. Formaldehyde treatment of lymphocytes increased the number of B cells detectable to 25.5% of the lymphocyte population. Study of T-enriched and B-enriched populations showed that the observed increase in B cell reactivity was real and not due to MAG-rosetting T cells. A one-stage procedure for T and B lymphocyte separation is described.", "contents": "Immunoglobulin-positive mononuclear cells in human peripheral blood: detection by mixed anti-globulin and direct anti-globulin-rosetting reactions. Ig-bearing mononuclear cells were identified in Ficoll-Hypaque preparations of human peripheral blood by using mixed anti-globulin (MAG) and direct anti-globulin rosettes; indicator cells consisted of sheep erythrocytes coated with human F(ab')2 or anti-F(ab')2 antibody, respectively. Of the cell population isolated from 10 normal subjects, a mean of 68% was lymphocytes. However, fewer than 50% of the cells with detectable surface Ig were lymphocytes. On viable cell preparations using chromic chloride-treated sheep erythrocytes (CrCl3SRBC) coated with anti-F(ab')2 antibody, a mean of 20.1% of the lymphocytes formed rosettes, i.e., were B. Up to 6% of peripheral blood lymphocytes formed mixed Ig-rosettes and E-rosettes. On viable lymphocytes using F(ab')2-coated CrCl3SRBC, MAG rosettes were insensitive in detection of B lymphocytes. Formaldehyde treatment of lymphocytes increased the number of B cells detectable to 25.5% of the lymphocyte population. Study of T-enriched and B-enriched populations showed that the observed increase in B cell reactivity was real and not due to MAG-rosetting T cells. A one-stage procedure for T and B lymphocyte separation is described."}
{"id": "PMID:1083874", "title": "Macrophage requirements of CR- and CR+ B lymphocytes for antibody production in vitro.", "content": "A Sephadex G-10 column coated with antigen-antibody complexes and complement retains complement receptor-bearing (CR+) mouse spleen cells. The effluent is rich in thymus-derived cells (T cells), and contains bone marrow-derived cells (B cells) which carry surface immunoglobulin (Ig), Ir-associated antigen (Ia), and Fc receptors, but no complement receptors (CR-). Although both unfractionated and CR- B cell populations are capable of producing antibody to red cell antigens, they differ in their requirements for the initiation of the response. Unfractionated B cells cooperate with primed as well as unprimed helper T cells; macrophages are required for this cooperation but can be replaced by 2-mercaptoethanol. CR- B cells cooperate with primed but not with unprimed T cells provided macrophages are added to cultures. After addition of culture supernatant from BCG-activated macrophages CR- B cells cooperate with both unprimed and primed T helper cells.", "contents": "Macrophage requirements of CR- and CR+ B lymphocytes for antibody production in vitro. A Sephadex G-10 column coated with antigen-antibody complexes and complement retains complement receptor-bearing (CR+) mouse spleen cells. The effluent is rich in thymus-derived cells (T cells), and contains bone marrow-derived cells (B cells) which carry surface immunoglobulin (Ig), Ir-associated antigen (Ia), and Fc receptors, but no complement receptors (CR-). Although both unfractionated and CR- B cell populations are capable of producing antibody to red cell antigens, they differ in their requirements for the initiation of the response. Unfractionated B cells cooperate with primed as well as unprimed helper T cells; macrophages are required for this cooperation but can be replaced by 2-mercaptoethanol. CR- B cells cooperate with primed but not with unprimed T cells provided macrophages are added to cultures. After addition of culture supernatant from BCG-activated macrophages CR- B cells cooperate with both unprimed and primed T helper cells."}
{"id": "PMID:1083875", "title": "The function of macrophages in antigen recognition by guinea pig T lymphocytes. III. Genetic analysis of the antigens mediating macrophage-T lymphocyte interaction.", "content": "Activation of immune T lymphocytes by antigen-pulsed macrophages is mediated by the Ia antigens of the guinea pig MHC or the products of closely linked genes. Studies using combinations of macrophages and T cells derived from outbred animals with different Ia antigens and/or Ir gene products have demonstrated that sharing of Ir gene products between macrophage and T cells is not sufficient for effective macrophage-T cell interaction. The role of the Ia antigens in the absence of the linked Ir gene products could not be directly examined because we were unable to identify an animal which bore the full complement of Ia antigens in the absence of the Ir gene that is normally associated with them. The results of these studies support the concept of the functional expression of the Ir gene product in the macrophage.", "contents": "The function of macrophages in antigen recognition by guinea pig T lymphocytes. III. Genetic analysis of the antigens mediating macrophage-T lymphocyte interaction. Activation of immune T lymphocytes by antigen-pulsed macrophages is mediated by the Ia antigens of the guinea pig MHC or the products of closely linked genes. Studies using combinations of macrophages and T cells derived from outbred animals with different Ia antigens and/or Ir gene products have demonstrated that sharing of Ir gene products between macrophage and T cells is not sufficient for effective macrophage-T cell interaction. The role of the Ia antigens in the absence of the linked Ir gene products could not be directly examined because we were unable to identify an animal which bore the full complement of Ia antigens in the absence of the Ir gene that is normally associated with them. The results of these studies support the concept of the functional expression of the Ir gene product in the macrophage."}
{"id": "PMID:1083876", "title": "Evolution of the lymphoid system. I. Evidence for lymphocyte heterogeneity in rainbow trout revealed by the organ distribution of mitogenic responses.", "content": "Leukocytes from the various lymphoid tissues of rainbow trout (RBT) were tested for their capacity to respond to the lymphocyte mitogens concanavalin A (Con A), lipopolysaccharide (LPS), and purified protein derivative of tuberculin (PPD). Thymocytes responded to Con A but not to LPS or PPD. In contrast, leukocytes from anterior kidney were stimulated with LPS but not with Con A or PPD. Cells from spleen and peripheral blood were stimulated by each mitogen. However, the degree of stimulation at optimally stimulatory concentrations of each mitogen was distinctive. The finding that the patterns of mitogenic responses of cells from each tissue were significantly different suggested that there is lymphoid heterogeneity in the RBT with a unique tissue distribution. The species source of serum utilized as a medium supplement appeared to be capable of markedly affecting mitogenesis. Thus, LPS and PPD stimulation occurred in medium supplemented with rainbow trout serum (RBTS). On the other hand, LPS and PPD stimulation was not observed in medium supplemented with fetal bovine serum (FBS), with the exception of peripheral blood leukocytes which were stimulated by LPS in culture medium supplemented with FBS. Con A stimulated leukocytes from each lymphoid tissue in medium supplemented with RBTS and, with the exception of cells from anterior kidney, also stimulated cells from each tissue in medium supplemented with FBS. The kinetic profiles of the responses of peripheral blood leukocytes to Con A, LPS, and PPD suggested that the extent as well as the time required for maximal stimulation was dependent on the dose of mitogen.", "contents": "Evolution of the lymphoid system. I. Evidence for lymphocyte heterogeneity in rainbow trout revealed by the organ distribution of mitogenic responses. Leukocytes from the various lymphoid tissues of rainbow trout (RBT) were tested for their capacity to respond to the lymphocyte mitogens concanavalin A (Con A), lipopolysaccharide (LPS), and purified protein derivative of tuberculin (PPD). Thymocytes responded to Con A but not to LPS or PPD. In contrast, leukocytes from anterior kidney were stimulated with LPS but not with Con A or PPD. Cells from spleen and peripheral blood were stimulated by each mitogen. However, the degree of stimulation at optimally stimulatory concentrations of each mitogen was distinctive. The finding that the patterns of mitogenic responses of cells from each tissue were significantly different suggested that there is lymphoid heterogeneity in the RBT with a unique tissue distribution. The species source of serum utilized as a medium supplement appeared to be capable of markedly affecting mitogenesis. Thus, LPS and PPD stimulation occurred in medium supplemented with rainbow trout serum (RBTS). On the other hand, LPS and PPD stimulation was not observed in medium supplemented with fetal bovine serum (FBS), with the exception of peripheral blood leukocytes which were stimulated by LPS in culture medium supplemented with FBS. Con A stimulated leukocytes from each lymphoid tissue in medium supplemented with RBTS and, with the exception of cells from anterior kidney, also stimulated cells from each tissue in medium supplemented with FBS. The kinetic profiles of the responses of peripheral blood leukocytes to Con A, LPS, and PPD suggested that the extent as well as the time required for maximal stimulation was dependent on the dose of mitogen."}
{"id": "PMID:1083877", "title": "Differentiation of lymphoid cells: B cell as a direct target and T cell as a regulator in lipopolysaccharide-enhanced induction of immunoglobulin production.", "content": "The cells involved in the stimulatory effect of bacterial lipopolysaccharide (LPS) on the induction of immunoglobulin (Ig) production by rabbit spleen cells cultured in the absence of antigen has been analyzed. Addition of LPS caused a several-fold enhancement of both DNA synthesis and Ig production. These enhanced activities were not significantly affected by depletion of adherent cells in the spleen cell population. Although inactivation of splenic T cells by anti-thymocyte serum (ATS) treatment did not affect the enhancement of DNA synthesis due to LPS, such treatment did adversely affect the enhancement of Ig production by LPS. Furthermore, the enhancement of Ig production of ATS-treated spleen cells by LPS was found to be dependent on the number of thymocytes added. In addition, the prior incubation of ATS-treated spleen cells with LPS resulted in effective enhancement of Ig production when such ATS-treated spleen cells and thymocytes were combined after removal of LPS. An identical experiment, except that thymocytes instead of ATS-treated spleen cells received the prior incubation with LPS, did not result in enhancement of Ig production. Finally, the enhanced Ig production due to LPS was inhibited by hydroxyurea, a known inhibitor of cellular DNA synthesis. The relationship between the mitogenic activity of LPS on B cells, the regulatory function of T cells, and the enhancement of Ig production by LPS is discussed in relation to the contrasting reports concerning the cellular target of LPS.", "contents": "Differentiation of lymphoid cells: B cell as a direct target and T cell as a regulator in lipopolysaccharide-enhanced induction of immunoglobulin production. The cells involved in the stimulatory effect of bacterial lipopolysaccharide (LPS) on the induction of immunoglobulin (Ig) production by rabbit spleen cells cultured in the absence of antigen has been analyzed. Addition of LPS caused a several-fold enhancement of both DNA synthesis and Ig production. These enhanced activities were not significantly affected by depletion of adherent cells in the spleen cell population. Although inactivation of splenic T cells by anti-thymocyte serum (ATS) treatment did not affect the enhancement of DNA synthesis due to LPS, such treatment did adversely affect the enhancement of Ig production by LPS. Furthermore, the enhancement of Ig production of ATS-treated spleen cells by LPS was found to be dependent on the number of thymocytes added. In addition, the prior incubation of ATS-treated spleen cells with LPS resulted in effective enhancement of Ig production when such ATS-treated spleen cells and thymocytes were combined after removal of LPS. An identical experiment, except that thymocytes instead of ATS-treated spleen cells received the prior incubation with LPS, did not result in enhancement of Ig production. Finally, the enhanced Ig production due to LPS was inhibited by hydroxyurea, a known inhibitor of cellular DNA synthesis. The relationship between the mitogenic activity of LPS on B cells, the regulatory function of T cells, and the enhancement of Ig production by LPS is discussed in relation to the contrasting reports concerning the cellular target of LPS."}
{"id": "PMID:1083878", "title": "Structural requirements for arthritogenicity of peptidoglycans from Staphylococcus aureus and Lactobacillus plant arum and analogous synthetic compounds.", "content": "The comparative studies on the arthritogenicity of chemically well defined peptidoglycans (PG)2 from the cell walls of Staphylococcus aureus (FDA 209P) and Lactobacillus plantarum (ATCC 8014) showed that 1) a polymer of disaccharide-peptide with or without the presence of N-acetylglucosaminyl-ribitol-teichoic acid produced severe arthritis; 2) oligosaccharide-peptides with or without the special structure (N-acetylglucosaminyl-ribitol-teichoic acid in S. aureus, a polymer of rhamnose and glucose in L. plantarum) produced severe disease; 3) disaccharide-heptapeptide-disaccharide with or without the presence of either glucose-ribitol-teichoic acid or a polymer of rhamnose and glucose appeared to be arthritogenic but much less effective for disease production; 4) N-acetylmuramyl-heptapeptide-N-acetylmuramic acid and disaccharide-hexapeptide were non-arthritogenic; 5) none of the synthetic N-acetylmuramyl-peptides, including tetrapeptide, produced the disease. Thus it is concluded that arthritis-inducing activity is related to the peptidoglycan moiety but not to the special structure, and the most important moiety responsible for disease production may be located in a chain length of two or more disaccharide units on PG subunits. However, it is discussed that non-arthritogenicity of peptidoglycans, including synthetic compounds, may be due to a lack of adjuvanticity in rats rather than a lack of antigenic determinant(s) responsible for production of arthritis.", "contents": "Structural requirements for arthritogenicity of peptidoglycans from Staphylococcus aureus and Lactobacillus plant arum and analogous synthetic compounds. The comparative studies on the arthritogenicity of chemically well defined peptidoglycans (PG)2 from the cell walls of Staphylococcus aureus (FDA 209P) and Lactobacillus plantarum (ATCC 8014) showed that 1) a polymer of disaccharide-peptide with or without the presence of N-acetylglucosaminyl-ribitol-teichoic acid produced severe arthritis; 2) oligosaccharide-peptides with or without the special structure (N-acetylglucosaminyl-ribitol-teichoic acid in S. aureus, a polymer of rhamnose and glucose in L. plantarum) produced severe disease; 3) disaccharide-heptapeptide-disaccharide with or without the presence of either glucose-ribitol-teichoic acid or a polymer of rhamnose and glucose appeared to be arthritogenic but much less effective for disease production; 4) N-acetylmuramyl-heptapeptide-N-acetylmuramic acid and disaccharide-hexapeptide were non-arthritogenic; 5) none of the synthetic N-acetylmuramyl-peptides, including tetrapeptide, produced the disease. Thus it is concluded that arthritis-inducing activity is related to the peptidoglycan moiety but not to the special structure, and the most important moiety responsible for disease production may be located in a chain length of two or more disaccharide units on PG subunits. However, it is discussed that non-arthritogenicity of peptidoglycans, including synthetic compounds, may be due to a lack of adjuvanticity in rats rather than a lack of antigenic determinant(s) responsible for production of arthritis."}
{"id": "PMID:1083879", "title": "B lymphocyte differentiation induced by lipopolysaccharide. III. Suppression of B cell maturation by anti-mouse immunoglobulin antibodies.", "content": "Lipopolysaccharide-(LPS) induced differentiation of mouse B lymphocytes to cells synthesizing large amounts of cytoplasmic IgM and IgG2 could be suppressed by antibodies to mu-chains. Maximal inhibition of LPS-induced differentiation was associated with increased cellular proliferation as measured by incorporation of 3H-thymidine, whereas treatment with anti-mu alone over a wide dosage range did not stimulate cellular proliferation. Spleen cells from newborn mice were suppressed by concentrations of anti-mu several hundred-fold lower than required for adult spleen cells; the adult pattern of susceptibility to suppression was acquired by 1 week of age. No significant differences in susceptibility to anti-mu were found in comparisons of adult spleen, lymph node, bone marrow, and Peyer's patch lymphocytes.", "contents": "B lymphocyte differentiation induced by lipopolysaccharide. III. Suppression of B cell maturation by anti-mouse immunoglobulin antibodies. Lipopolysaccharide-(LPS) induced differentiation of mouse B lymphocytes to cells synthesizing large amounts of cytoplasmic IgM and IgG2 could be suppressed by antibodies to mu-chains. Maximal inhibition of LPS-induced differentiation was associated with increased cellular proliferation as measured by incorporation of 3H-thymidine, whereas treatment with anti-mu alone over a wide dosage range did not stimulate cellular proliferation. Spleen cells from newborn mice were suppressed by concentrations of anti-mu several hundred-fold lower than required for adult spleen cells; the adult pattern of susceptibility to suppression was acquired by 1 week of age. No significant differences in susceptibility to anti-mu were found in comparisons of adult spleen, lymph node, bone marrow, and Peyer's patch lymphocytes."}
{"id": "PMID:1083880", "title": "The merrit alloantigenic system of human B lymphocytes: evidence for thirteen possible factors including one six-member segregant series.", "content": "An analysis of the typing results of a 70-member chronic lymphatic leukemia B cell panel revealed evidence for 13 possible groups of the Merrit alloantigenic system. Six of these appeared possibly allelic and may represent a segregant series. The CLL panel was also fully typed for HLA and some degree of linkage dysequilibrium between Merrit and HLA seemed apparent from the data. Merrit antibodies can be absorbed out with selected surface membrane immunoglobulin (SMIG)-positive normal lymphocytes and less so or not at all with E rosette-forming T cells or Fc-positive SMIG-negative lymphocytes.", "contents": "The merrit alloantigenic system of human B lymphocytes: evidence for thirteen possible factors including one six-member segregant series. An analysis of the typing results of a 70-member chronic lymphatic leukemia B cell panel revealed evidence for 13 possible groups of the Merrit alloantigenic system. Six of these appeared possibly allelic and may represent a segregant series. The CLL panel was also fully typed for HLA and some degree of linkage dysequilibrium between Merrit and HLA seemed apparent from the data. Merrit antibodies can be absorbed out with selected surface membrane immunoglobulin (SMIG)-positive normal lymphocytes and less so or not at all with E rosette-forming T cells or Fc-positive SMIG-negative lymphocytes."}
{"id": "PMID:1083883", "title": "Bernard-Soulier syndrome: a new platelet glycoprotein abnormality. Its relationship with platelet adhesion to subendothelium and with the factor VIII von Willebrand protein.", "content": "A decreased platelet adhesion to rabbit aorta subendothelium (Baumgartner technique) is confirmed in the Bernard Soulier (giant platelet) syndrome. Electron microscope techniques using a purified antibody against Factor VIII/von Willebrand protein, revealed an apparently normal presence of the Factor VIII/von Willebrand protein on the Bernard Soulier platelets. Electrophoretic characterization of the major protein and glycoprotein components of the Bernard Soulier platelets following sodium dodecyl sulfate solubilization indicated a relatively normal protein content but suggested a reduced content of the 155,000 molecular weight major platelet glycoprotein. This was confirmed by a reduced release of high molecular weight acidic glycopeptides following incubation of washed Bernard Soulier platelets with trypsin. It is proposed that this abnormality may be related to the previously reported reduced sialic acid content and the reduced electrophoretic mobility of the Bernard Soulier platelets and that a glycoprotein reduced or abnormal in the Bernard Soulier platelets is necessary for the normal adhesion of platelets to subendothelium.", "contents": "Bernard-Soulier syndrome: a new platelet glycoprotein abnormality. Its relationship with platelet adhesion to subendothelium and with the factor VIII von Willebrand protein. A decreased platelet adhesion to rabbit aorta subendothelium (Baumgartner technique) is confirmed in the Bernard Soulier (giant platelet) syndrome. Electron microscope techniques using a purified antibody against Factor VIII/von Willebrand protein, revealed an apparently normal presence of the Factor VIII/von Willebrand protein on the Bernard Soulier platelets. Electrophoretic characterization of the major protein and glycoprotein components of the Bernard Soulier platelets following sodium dodecyl sulfate solubilization indicated a relatively normal protein content but suggested a reduced content of the 155,000 molecular weight major platelet glycoprotein. This was confirmed by a reduced release of high molecular weight acidic glycopeptides following incubation of washed Bernard Soulier platelets with trypsin. It is proposed that this abnormality may be related to the previously reported reduced sialic acid content and the reduced electrophoretic mobility of the Bernard Soulier platelets and that a glycoprotein reduced or abnormal in the Bernard Soulier platelets is necessary for the normal adhesion of platelets to subendothelium."}
{"id": "PMID:1083884", "title": "Simplified alpha1-antitrypsin phenotyping by immunofixation of acid-starch gels.", "content": "Immunofixation of acid-starch gels represents a simplified method for alpha1-antitrypsin phenotyping. This technique reduces the amount of work and time involved in phenotyping by eliminating the need for crossed-immunoelectrophoresis. Its major purpose is to directly enhance the staining quality of the minor bands, especially the Z bands on the acid-starch gel. It is hoped that this modified method will allow more wide-spread use of antitrypsin phenotyping, especially in the routine clinical chemistry laboratory.", "contents": "Simplified alpha1-antitrypsin phenotyping by immunofixation of acid-starch gels. Immunofixation of acid-starch gels represents a simplified method for alpha1-antitrypsin phenotyping. This technique reduces the amount of work and time involved in phenotyping by eliminating the need for crossed-immunoelectrophoresis. Its major purpose is to directly enhance the staining quality of the minor bands, especially the Z bands on the acid-starch gel. It is hoped that this modified method will allow more wide-spread use of antitrypsin phenotyping, especially in the routine clinical chemistry laboratory."}
{"id": "PMID:1083885", "title": "Embryological evidence for a possible polyphyletic origin of the recent amphibians.", "content": "The markedly different mode of mesoderm formation in anuran and urodelan amphibians (which is related to the early double-layered nature of the anuran blastula wall in contrast to its single-layered nature in the urodeles), but particularly the fundamentally different place and mode of origin of the primordial germ cells in the two groups of amphibians, strongly pleads in favour of a very ancient bifurcation in the phylogenetic history of the two groups, even suggesting a polyphyletic origin from different ancestral fishes.", "contents": "Embryological evidence for a possible polyphyletic origin of the recent amphibians. The markedly different mode of mesoderm formation in anuran and urodelan amphibians (which is related to the early double-layered nature of the anuran blastula wall in contrast to its single-layered nature in the urodeles), but particularly the fundamentally different place and mode of origin of the primordial germ cells in the two groups of amphibians, strongly pleads in favour of a very ancient bifurcation in the phylogenetic history of the two groups, even suggesting a polyphyletic origin from different ancestral fishes."}
{"id": "PMID:1083886", "title": "The initiation of diving apnoea in the frog, Rana pipiens.", "content": "1. Diving apnoea in Rana pipiens was initiated by submerging the external nares. As the water level was raised above the frog, both buccal and lung pressure increased by an amount corresponding to the water head. During submergence the external nares remained closed, although the apnoeic period was punctuated by ventilation movements which moved gas between the lungs and buccal cavity. 2. Bilateral section of the ophthalmic nerves did not alter the normal pattern of ventilation in air, although it often resulted in the intake of water into the buccal cavity on submergence. Introduction of water into the buccal cavity, either naturally as in denervates or by injection through a catheter in intact frogs, triggered sustained electromyographical activity in some respiratory muscles. 3. Electroneurograms recorded from the cut peripheral end of an ophthalmic nerve showed that receptors in the external narial region were stimulated by movement of a water meniscus across them. Activity could also be recorded in the ophthalmic nerve in response to water flow past the submerged nares. Punctate stimulation of the narial region confirmed that these receptors were mechanosensitive. 4. Bilateral electrical stimulation of the central ends of cut ophthalmic nerves in lightly anaesthetized frogs caused apnoea with a latency of less than 200 ms. The external nares remained closed throughout the period of stimulation although buccal pressure events, resembling underwater ventilation movements, occurred when stimulation was prolonged.", "contents": "The initiation of diving apnoea in the frog, Rana pipiens. 1. Diving apnoea in Rana pipiens was initiated by submerging the external nares. As the water level was raised above the frog, both buccal and lung pressure increased by an amount corresponding to the water head. During submergence the external nares remained closed, although the apnoeic period was punctuated by ventilation movements which moved gas between the lungs and buccal cavity. 2. Bilateral section of the ophthalmic nerves did not alter the normal pattern of ventilation in air, although it often resulted in the intake of water into the buccal cavity on submergence. Introduction of water into the buccal cavity, either naturally as in denervates or by injection through a catheter in intact frogs, triggered sustained electromyographical activity in some respiratory muscles. 3. Electroneurograms recorded from the cut peripheral end of an ophthalmic nerve showed that receptors in the external narial region were stimulated by movement of a water meniscus across them. Activity could also be recorded in the ophthalmic nerve in response to water flow past the submerged nares. Punctate stimulation of the narial region confirmed that these receptors were mechanosensitive. 4. Bilateral electrical stimulation of the central ends of cut ophthalmic nerves in lightly anaesthetized frogs caused apnoea with a latency of less than 200 ms. The external nares remained closed throughout the period of stimulation although buccal pressure events, resembling underwater ventilation movements, occurred when stimulation was prolonged."}
{"id": "PMID:1083887", "title": "Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses.", "content": "Concanavalin A, a nonspecific polyclonal activator of T lymphocytes, activates Lyl and Ly23 subclasses to the same degree. After activation, the Ly23 subclass, but not the Lyl subclass, has the following properties: (a) Suppression of the antibody response to sheep erythrocytes (SRBC) in vitro. (b) Production of a soluble factor that suppresses the anti-SRBC response in vitro. (c) Suppression of the generation of cell-mediated cytotoxicity to H-2 target cells in vitro. Con A-activated cells of the Lyl subclass, but not the Ly23 subclass, express helper function in the anti-SRBC response in vitro. Because the intact Con A-stimulated T-cell population contains both cell types, these cells do not exert detectable helper effects in an anti-SRBC system in vitro, because the helper effect of Lyl cells is masked by the suppressor effect of the Ly23 cells. Each function is revealed by eliminating one or the other population with the relevant Ly antiserum. The resting T-cell population, before activation by Con A, also contains already programmed Lyl and Ly23 cells with similar helper and suppressor potentials, respectively. This is revealed by experiments with Ly subclasses which have been separated from the resting T-cell population and then stimulated by Con A. Thus helper and suppressor functions, as expressed in these systems, are manifestations of separate T-cell-differentiative pathways and do not depend upon stimulation of the cells by antigen.", "contents": "Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses. Concanavalin A, a nonspecific polyclonal activator of T lymphocytes, activates Lyl and Ly23 subclasses to the same degree. After activation, the Ly23 subclass, but not the Lyl subclass, has the following properties: (a) Suppression of the antibody response to sheep erythrocytes (SRBC) in vitro. (b) Production of a soluble factor that suppresses the anti-SRBC response in vitro. (c) Suppression of the generation of cell-mediated cytotoxicity to H-2 target cells in vitro. Con A-activated cells of the Lyl subclass, but not the Ly23 subclass, express helper function in the anti-SRBC response in vitro. Because the intact Con A-stimulated T-cell population contains both cell types, these cells do not exert detectable helper effects in an anti-SRBC system in vitro, because the helper effect of Lyl cells is masked by the suppressor effect of the Ly23 cells. Each function is revealed by eliminating one or the other population with the relevant Ly antiserum. The resting T-cell population, before activation by Con A, also contains already programmed Lyl and Ly23 cells with similar helper and suppressor potentials, respectively. This is revealed by experiments with Ly subclasses which have been separated from the resting T-cell population and then stimulated by Con A. Thus helper and suppressor functions, as expressed in these systems, are manifestations of separate T-cell-differentiative pathways and do not depend upon stimulation of the cells by antigen."}
{"id": "PMID:1083888", "title": "Separation of helper T cells from suppressor T cells expressing different Ly components. II. Activation by antigen: after immunization, antigen-specific suppressor and helper activities are mediated by distinct T-cell subclasses.", "content": "Cells of the Lyl subclass generate helper activity in both primary and secondary responses to sheep erythrocytes (SRBC). In contrast, after priming with SRBC, cells of the Ly-2+ subclasses, in particular Ly23 cells, have suppressive activity. The degree of Ly23-mediated suppression is directly proportional to the amount of antigen (SRBC) used for priming. Suppression by Ly23 cells is specific, because Ly23 cells from SRBC-primed animals do not suppress the response to horse erythrocytes, and vice versa. Thus, both cytotoxic and specific suppressor functions are mediated by T cells of a subclass, provisionally designated TCS, which can be distinguished from helper T cells (TH), by their Ly phenotypes. It remains to be determined whether killing and suppression are functionally interrelated properties of a single Ly23 subclass, or whether the Ly23 population comprises two subclasses whose surface phenotypes are not yet distinguishable by immunogenetic criteria.", "contents": "Separation of helper T cells from suppressor T cells expressing different Ly components. II. Activation by antigen: after immunization, antigen-specific suppressor and helper activities are mediated by distinct T-cell subclasses. Cells of the Lyl subclass generate helper activity in both primary and secondary responses to sheep erythrocytes (SRBC). In contrast, after priming with SRBC, cells of the Ly-2+ subclasses, in particular Ly23 cells, have suppressive activity. The degree of Ly23-mediated suppression is directly proportional to the amount of antigen (SRBC) used for priming. Suppression by Ly23 cells is specific, because Ly23 cells from SRBC-primed animals do not suppress the response to horse erythrocytes, and vice versa. Thus, both cytotoxic and specific suppressor functions are mediated by T cells of a subclass, provisionally designated TCS, which can be distinguished from helper T cells (TH), by their Ly phenotypes. It remains to be determined whether killing and suppression are functionally interrelated properties of a single Ly23 subclass, or whether the Ly23 population comprises two subclasses whose surface phenotypes are not yet distinguishable by immunogenetic criteria."}
{"id": "PMID:1083889", "title": "Ontogeny of B-lymphocyte function. II. Ability of endotoxin to increase the heterogeneity of affinity of the immune response of B lymphocytes from fetal mice.", "content": "The ontogeny of the functional capacity of B lymphocytes to generate a heterogeneous response to a haptenic determinant was studied by cell transfer techniques in LAF1 mice. Fetal liver, as a source of B lymphocytes, was transferred into adult, syngeneic, irradiated animals. All recipients received excess adult thymus cells so that T-cell activity did not limit the response and were immunized with DNP-BGG. The heterogeneity of avidity of their anti-DNP PFC response was assayed by hapten inhibition of plaque formation. Animals reconstituted with B lymphocytes from fetal donors produced a response that is highly restricted with respect to heterogeneity of affinity. Transfer studies using multiple fetal donors or mixtures of adult and neonatal cells for reconstitution suggest that the restriction in heterogeneity is not the consequence of suppressor T-lymphocyte activity. With animals reconstituted with B cells from day 16 or older fetal donors, injection of LPS together with antigen converted the response to a heterogeneous \"adult-type\" response. With animals reconstituted with B lymphocytes from day 14 fetal liver DxSO4, but not LPS, could convert the response to a highly heterogeneous one. Animals reconstituted with day 14 or 16 fetal liver as source of B lymphocytes were capable of producing a heterogeneous secondary response despite the fact that their primary response was of restricted heterogeneity. This implies the selection of high affinity B-memory cells, in the absence of high affinity PFC during the primary response with fetal B lymphocytes. Animals reconstituted with day 14 or 16 fetal liver produce only direct PFC, while animals reconstituted with day 18 fetal liver produce both direct and indirect PFC. Three differentiation events have therefore been defined in the functional development of B lymphocytes: (a) between day 14 and day 16 of fetal life they acquire responsiveness to LPS; (B) BETWEEN DAY 16 AND 18 OF FETAL DEVELOPMENT THEY ACQUire the capacity to produce indirect PFC; (C) between day 7 and 10 after birth they acquire the capacity to give a heterogeneous response after normal immunization. In addition, it was shown that LAF1 mice already have all of the information required to produce an \"adult-type\" heterogeneous anti-DNP response at day 14 of fetal life.", "contents": "Ontogeny of B-lymphocyte function. II. Ability of endotoxin to increase the heterogeneity of affinity of the immune response of B lymphocytes from fetal mice. The ontogeny of the functional capacity of B lymphocytes to generate a heterogeneous response to a haptenic determinant was studied by cell transfer techniques in LAF1 mice. Fetal liver, as a source of B lymphocytes, was transferred into adult, syngeneic, irradiated animals. All recipients received excess adult thymus cells so that T-cell activity did not limit the response and were immunized with DNP-BGG. The heterogeneity of avidity of their anti-DNP PFC response was assayed by hapten inhibition of plaque formation. Animals reconstituted with B lymphocytes from fetal donors produced a response that is highly restricted with respect to heterogeneity of affinity. Transfer studies using multiple fetal donors or mixtures of adult and neonatal cells for reconstitution suggest that the restriction in heterogeneity is not the consequence of suppressor T-lymphocyte activity. With animals reconstituted with B cells from day 16 or older fetal donors, injection of LPS together with antigen converted the response to a heterogeneous \"adult-type\" response. With animals reconstituted with B lymphocytes from day 14 fetal liver DxSO4, but not LPS, could convert the response to a highly heterogeneous one. Animals reconstituted with day 14 or 16 fetal liver as source of B lymphocytes were capable of producing a heterogeneous secondary response despite the fact that their primary response was of restricted heterogeneity. This implies the selection of high affinity B-memory cells, in the absence of high affinity PFC during the primary response with fetal B lymphocytes. Animals reconstituted with day 14 or 16 fetal liver produce only direct PFC, while animals reconstituted with day 18 fetal liver produce both direct and indirect PFC. Three differentiation events have therefore been defined in the functional development of B lymphocytes: (a) between day 14 and day 16 of fetal life they acquire responsiveness to LPS; (B) BETWEEN DAY 16 AND 18 OF FETAL DEVELOPMENT THEY ACQUire the capacity to produce indirect PFC; (C) between day 7 and 10 after birth they acquire the capacity to give a heterogeneous response after normal immunization. In addition, it was shown that LAF1 mice already have all of the information required to produce an \"adult-type\" heterogeneous anti-DNP response at day 14 of fetal life."}
{"id": "PMID:1083890", "title": "Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.", "content": "A human cell line established in culture from a histiocytic lymphoma patient synthesizes and secretes the monocyte-granulocyte specific enzyme lysozyme. 18 other human cell lines with characteristics of T-lymphocyte, B-lymphocyte, Burkitt's lymphoma, non-Burkitt's lymphoma, myeloma, and bone marrow epithelial cells were not associated with lysozyme. Among murine cell lines, lysozyme was produced by (a) three histiocytic lymphoma or macrophage lines, which mediate antibody-dependent phagocytosis and cytolysis; (b) myelomonocytic leukemia line which also secretes myeloid colony-stimulating factor; and (c) a spontaneous lymphoma and an Abelson leukemia virus-induced lymphoma. Lysozyme-negative lines include another Abelson lymphoma, myelomas, T lymphomas, and mastocytoma.", "contents": "Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. A human cell line established in culture from a histiocytic lymphoma patient synthesizes and secretes the monocyte-granulocyte specific enzyme lysozyme. 18 other human cell lines with characteristics of T-lymphocyte, B-lymphocyte, Burkitt's lymphoma, non-Burkitt's lymphoma, myeloma, and bone marrow epithelial cells were not associated with lysozyme. Among murine cell lines, lysozyme was produced by (a) three histiocytic lymphoma or macrophage lines, which mediate antibody-dependent phagocytosis and cytolysis; (b) myelomonocytic leukemia line which also secretes myeloid colony-stimulating factor; and (c) a spontaneous lymphoma and an Abelson leukemia virus-induced lymphoma. Lysozyme-negative lines include another Abelson lymphoma, myelomas, T lymphomas, and mastocytoma."}
{"id": "PMID:1083891", "title": "Cell-mediated immunity: delayed-type hypersensitivity and cytotoxic responses are mediated by different T-cell subclasses.", "content": "Cell-mediated immunity includes both the generation of cytotoxic cells and initiation of delayed-type hypersensitivity (DTH). The resting T-cell population, before stimulation by antigen, already contains cells of the Lyl subclass that are programmed to initiate DTH (and helper function) but not cytotoxic responses, as well as Ly23 cells which can generate killer activity (and suppressive function) but not DTH. The central implication of these findings is that the broad division between humoral and cell-mediated immune responses does not precisely correspond to the division of labor among T-cell subclasses. The relative contribution of DTH-competent Lyl cells and cytotoxic Ly23 cells to the classical homograft response remains to be determined.", "contents": "Cell-mediated immunity: delayed-type hypersensitivity and cytotoxic responses are mediated by different T-cell subclasses. Cell-mediated immunity includes both the generation of cytotoxic cells and initiation of delayed-type hypersensitivity (DTH). The resting T-cell population, before stimulation by antigen, already contains cells of the Lyl subclass that are programmed to initiate DTH (and helper function) but not cytotoxic responses, as well as Ly23 cells which can generate killer activity (and suppressive function) but not DTH. The central implication of these findings is that the broad division between humoral and cell-mediated immune responses does not precisely correspond to the division of labor among T-cell subclasses. The relative contribution of DTH-competent Lyl cells and cytotoxic Ly23 cells to the classical homograft response remains to be determined."}
{"id": "PMID:1083892", "title": "Cellular basis of neonatal induction of in vitro tolerance.", "content": "The LD and SD antigens of the major histocompatibility complex subserve differential roles in the induction of the proliferative phase in mixed lymphocyte culture and in the cytotoxic reaction seen in cell-mediated lympholysis. The present study suggests that they also behave differently in the neonatal induction of tolerance. SD antigens appear to induce tolerance in the cytotoxic T lymphocytes very effectively, whereas LD antigens (or the cytotoxic targets coded by genes in the I and/or S regions) are relatively ineffective in this regard. LD antigens presented neonatally are effective at inducing tolerance in the proliferating helper cells.", "contents": "Cellular basis of neonatal induction of in vitro tolerance. The LD and SD antigens of the major histocompatibility complex subserve differential roles in the induction of the proliferative phase in mixed lymphocyte culture and in the cytotoxic reaction seen in cell-mediated lympholysis. The present study suggests that they also behave differently in the neonatal induction of tolerance. SD antigens appear to induce tolerance in the cytotoxic T lymphocytes very effectively, whereas LD antigens (or the cytotoxic targets coded by genes in the I and/or S regions) are relatively ineffective in this regard. LD antigens presented neonatally are effective at inducing tolerance in the proliferating helper cells."}
{"id": "PMID:1083893", "title": "Fetal antigens on the surface of human lymphoid cells.", "content": "Human B-lymphoblastoid cells established in long-term culture from healthy adults carry surface components that are normally found in human fetal tissues at about 10 wk of age. These antigens are strongly expressed on neoplastic B lymphocytes but not on thymocytes or a cultured T-cell line. They are carried by a small subpopulation of normal adult peripheral blood lymphocytes as well.", "contents": "Fetal antigens on the surface of human lymphoid cells. Human B-lymphoblastoid cells established in long-term culture from healthy adults carry surface components that are normally found in human fetal tissues at about 10 wk of age. These antigens are strongly expressed on neoplastic B lymphocytes but not on thymocytes or a cultured T-cell line. They are carried by a small subpopulation of normal adult peripheral blood lymphocytes as well."}
{"id": "PMID:1083894", "title": "An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes.", "content": "The cell-mediated immune response has been generated in vitro with a polyacrylamide culture system which allows the segregation of foci (clones?) of cytotoxic lymphocytes. Using the method of limiting dilutions, the frequency of precursor cells in CBA spleen cells able to generate a cytotoxic response against DBA mastocytoma is estimated at 1 per 1,700 cells.", "contents": "An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes. The cell-mediated immune response has been generated in vitro with a polyacrylamide culture system which allows the segregation of foci (clones?) of cytotoxic lymphocytes. Using the method of limiting dilutions, the frequency of precursor cells in CBA spleen cells able to generate a cytotoxic response against DBA mastocytoma is estimated at 1 per 1,700 cells."}
{"id": "PMID:1083895", "title": "Density and distribution of tetrodotoxin receptors in normal and detubulated frog sartorius muscle.", "content": "Tetrodotoxin (TTX) binding was measured in muscles which were either in normal condition or which had been \"detubulated\" by glycerol-induced osmotic shock. In both cases the binding of TTX was found to saturate at high TTX concentrations. Maximum binding in normal fibers was 35 pmol/g wet weight, and that figure was reduced to 16 pmol/g after glycerol treatment. The dissociation constant for binding to the surface membrane was 3 nM, which is the range of values obtained by electrophysiological measurements of the effect of TTX on the maximum rate of rise of the action potential. The results suggest that the dissociation constant in the transverse tubules may be higher than that in the surface. Control experiments indicate that the effects of glycerol treatment are limited to the accessibility of the receptors to the toxin and result in no alteration of the affinity of the binding site. TTX receptors in the transverse tubules may be recovered after glycerol treatment by homogenization of the fibers. The measurements suggest that the density of sodium channels in surface membrane is about 175/muM2 and that in the transverse tubular membrane is 41-52/mum2.", "contents": "Density and distribution of tetrodotoxin receptors in normal and detubulated frog sartorius muscle. Tetrodotoxin (TTX) binding was measured in muscles which were either in normal condition or which had been \"detubulated\" by glycerol-induced osmotic shock. In both cases the binding of TTX was found to saturate at high TTX concentrations. Maximum binding in normal fibers was 35 pmol/g wet weight, and that figure was reduced to 16 pmol/g after glycerol treatment. The dissociation constant for binding to the surface membrane was 3 nM, which is the range of values obtained by electrophysiological measurements of the effect of TTX on the maximum rate of rise of the action potential. The results suggest that the dissociation constant in the transverse tubules may be higher than that in the surface. Control experiments indicate that the effects of glycerol treatment are limited to the accessibility of the receptors to the toxin and result in no alteration of the affinity of the binding site. TTX receptors in the transverse tubules may be recovered after glycerol treatment by homogenization of the fibers. The measurements suggest that the density of sodium channels in surface membrane is about 175/muM2 and that in the transverse tubular membrane is 41-52/mum2."}
{"id": "PMID:1083897", "title": "Normal rate of cerebrospinal fluid formation five years after bilateral choroid plexectomy. Case report.", "content": "A ventricular perfusion technique was used to determine the rate of cerebrospinal fluid (CSF) formation in a 5-year-old child who had undergone bilateral choroid plexectomy for communicating hydrocephalus during infancy. At the time of the study, the patient had a failed ventriculoperitoneal shunt and was suffering from progressive ventriculomegaly. The calculated rate of CSF formation, 0.35 ml/min +/- 0.02 standard deviation, was within normal limits.", "contents": "Normal rate of cerebrospinal fluid formation five years after bilateral choroid plexectomy. Case report. A ventricular perfusion technique was used to determine the rate of cerebrospinal fluid (CSF) formation in a 5-year-old child who had undergone bilateral choroid plexectomy for communicating hydrocephalus during infancy. At the time of the study, the patient had a failed ventriculoperitoneal shunt and was suffering from progressive ventriculomegaly. The calculated rate of CSF formation, 0.35 ml/min +/- 0.02 standard deviation, was within normal limits."}
{"id": "PMID:1083896", "title": "The effects of serotonergic and antiserotonergic drugs on the flexor reflex of spinal rat: a proposed model to evaluate the action on the central serotonin receptor.", "content": "The effects of serotonergic and antiserotonergic drugs on the hind limb flexor reflex (measured as a contraction of musculus tibialis anterior or as a flexion of the paw) in the spinal rat was studied. All serotonergic drugs used (L-5-hydroxytryptophan, L-tryptophan, LSD, fenfluramine, p-chloroamphetamine) stimulate the flexor reflex. Serotonin receptor blockers (cyproheptadine, WA-335, methergoline), which given alone are inactive, inhibit the stimulation induced by serotonergic drugs but do not influence that one caused by noradrenergic agents (amphetamine, clonidine). Both types of stimulation (serotonergic and noradrenergic) are antagonized by noradrenaline receptor blockers (phenoxybenzamine, clozapine). The antiserotonergic action of serotonin antagonists used was confirmed on the basis of the antagonism to the head twitches induced by L-5-hydroxytryptophan. The flexor reflex in the spinal rat may be a good model to evaluate the effect on the central (spinal cord) serotonin receptor and to differantiat this effect from that on the noradrenaline system.", "contents": "The effects of serotonergic and antiserotonergic drugs on the flexor reflex of spinal rat: a proposed model to evaluate the action on the central serotonin receptor. The effects of serotonergic and antiserotonergic drugs on the hind limb flexor reflex (measured as a contraction of musculus tibialis anterior or as a flexion of the paw) in the spinal rat was studied. All serotonergic drugs used (L-5-hydroxytryptophan, L-tryptophan, LSD, fenfluramine, p-chloroamphetamine) stimulate the flexor reflex. Serotonin receptor blockers (cyproheptadine, WA-335, methergoline), which given alone are inactive, inhibit the stimulation induced by serotonergic drugs but do not influence that one caused by noradrenergic agents (amphetamine, clonidine). Both types of stimulation (serotonergic and noradrenergic) are antagonized by noradrenaline receptor blockers (phenoxybenzamine, clozapine). The antiserotonergic action of serotonin antagonists used was confirmed on the basis of the antagonism to the head twitches induced by L-5-hydroxytryptophan. The flexor reflex in the spinal rat may be a good model to evaluate the effect on the central (spinal cord) serotonin receptor and to differantiat this effect from that on the noradrenaline system."}
{"id": "PMID:1083898", "title": "A new concept in the control of acute gingival hemorrhage.", "content": "The topical application of epsilon-aminocaproic acid may be life-saving therapy for control of acute gingival hemorrhage in patients who are debilitated because of systemic diseases. When low doses were used, there have been no reports of adverse reactions.", "contents": "A new concept in the control of acute gingival hemorrhage. The topical application of epsilon-aminocaproic acid may be life-saving therapy for control of acute gingival hemorrhage in patients who are debilitated because of systemic diseases. When low doses were used, there have been no reports of adverse reactions."}
{"id": "PMID:1083902", "title": "Continuous bilateral electrophrenic pacing in an infant with total diaphragmatic paralysis.", "content": "An infant with bilateral diaphragmatic paralysis, etiology undetermined, is presented in whom bilateral intrathoracic phrenic nerve pacemakers were inserted. He was maintained with continuous bilateral electrophrenic pacing for 142 days. A review of the literature by the authors has not revealed any previously reported cases.", "contents": "Continuous bilateral electrophrenic pacing in an infant with total diaphragmatic paralysis. An infant with bilateral diaphragmatic paralysis, etiology undetermined, is presented in whom bilateral intrathoracic phrenic nerve pacemakers were inserted. He was maintained with continuous bilateral electrophrenic pacing for 142 days. A review of the literature by the authors has not revealed any previously reported cases."}
{"id": "PMID:1083904", "title": "Safety aspects of mandibular lingual surgery.", "content": "Mandibular lingual surgery is commonly used in periodontal practice. There are inherent risks in such surgery which are illustrated by a case report described here. This was a case in which an emergency tracheotomy was needed after a minor procedure which involved lingual tissues. The potential problems of any form of lingual surgery are discussed. Various other surgical procedures in this region are examined in order to establish some surgical principles which may be of use to periodontists.", "contents": "Safety aspects of mandibular lingual surgery. Mandibular lingual surgery is commonly used in periodontal practice. There are inherent risks in such surgery which are illustrated by a case report described here. This was a case in which an emergency tracheotomy was needed after a minor procedure which involved lingual tissues. The potential problems of any form of lingual surgery are discussed. Various other surgical procedures in this region are examined in order to establish some surgical principles which may be of use to periodontists."}
{"id": "PMID:1083905", "title": "Effects of ethanol on the permeability of frog skin.", "content": "Ethanol (3%) decreases the potential difference and short-circuit current across the isolated frog skin in chloride Ringer's solution. Unidirectional fluxes of Na and Cl indicate that the drop in short-circuit current is due to an inhibition of the sodium influx. However, ethanol had no effect on the electrical parameters or sodium fluxes, when the frog skin was bathed in chloride-free solutions on both sides or the outside alone. The ethanol response is anion-dependent. In addition, chloride-free media in the inside bathing solution reduced the short-circuit current, indicating a sodium transport pathway which is dependent on chloride and confirming previous data in the literature. Other anions such as sulfate and nitrate could not substitute for chloride. The vasopressin-induced natriferic response and the ethanol effect were found to work independently of each other and different pathways of action are suggested for these agents. The intracellular sodium content of the isolated frog skin epithelium increased and potassium decreased in the presence of the Na-K adenosine triphosphatase inhibitor, ouabain, whereas ethanol or amiloride had no effect. The oxygen consumption of the isolated frog skin was unaffected by up to 10% ethanol. A general metabolic action is probably thus not mediating the response. Urea, in iso-osmotic concentrations to the ethanol, did not mimic its effect. Tritiated water fluxes (in the absence of an osmotic gradient) were reduced by 30% in the presence of 3% ethanol. It is suggested that ethanol may impede the flow of water across frog skin by a physicochemical interaction with membrane pores and the water molecules. The permeability coefficient (Ktrans) for ethanol was found to be 10 times smaller than the Ktrans for water.", "contents": "Effects of ethanol on the permeability of frog skin. Ethanol (3%) decreases the potential difference and short-circuit current across the isolated frog skin in chloride Ringer's solution. Unidirectional fluxes of Na and Cl indicate that the drop in short-circuit current is due to an inhibition of the sodium influx. However, ethanol had no effect on the electrical parameters or sodium fluxes, when the frog skin was bathed in chloride-free solutions on both sides or the outside alone. The ethanol response is anion-dependent. In addition, chloride-free media in the inside bathing solution reduced the short-circuit current, indicating a sodium transport pathway which is dependent on chloride and confirming previous data in the literature. Other anions such as sulfate and nitrate could not substitute for chloride. The vasopressin-induced natriferic response and the ethanol effect were found to work independently of each other and different pathways of action are suggested for these agents. The intracellular sodium content of the isolated frog skin epithelium increased and potassium decreased in the presence of the Na-K adenosine triphosphatase inhibitor, ouabain, whereas ethanol or amiloride had no effect. The oxygen consumption of the isolated frog skin was unaffected by up to 10% ethanol. A general metabolic action is probably thus not mediating the response. Urea, in iso-osmotic concentrations to the ethanol, did not mimic its effect. Tritiated water fluxes (in the absence of an osmotic gradient) were reduced by 30% in the presence of 3% ethanol. It is suggested that ethanol may impede the flow of water across frog skin by a physicochemical interaction with membrane pores and the water molecules. The permeability coefficient (Ktrans) for ethanol was found to be 10 times smaller than the Ktrans for water."}
{"id": "PMID:1083906", "title": "Components of human split ejaculates. II. Enzymes and proteinase inhibitors.", "content": "Lysozyme, alpha-amylase, neutral proteinase and plasminogen activator were most concentrated in the initial portion of the ejaculate that consists mostly of Cowper's gland and prostate gland fluids as well as spermatozoa. The concentration of the high molecular weight proteinase inhibitors, alpha1-antitrypsin and alpha1X-antichymotrypsin, was essentially unaltered throughout the ejaculate fractions, although their absolute amounts showed an increase towards the final fraction. By contrast, the total inhibitory activity towards pancreatic trypsin was highest both in concentration and amount in the last fraction, thus indicating that the seminal vesicles are its primary source. Plasminogen, prothrombin, Factor XIII, and the proteinase inhibitors antithrombin III, alpha2-macroglobulin, inter-alpha-trypsin inhibitor and C1S-inactivator could not be detected immunochemically in whole ejaculates, and indicates the dissimilarity between the coagulation/liquefaction processes of semen and blood.", "contents": "Components of human split ejaculates. II. Enzymes and proteinase inhibitors. Lysozyme, alpha-amylase, neutral proteinase and plasminogen activator were most concentrated in the initial portion of the ejaculate that consists mostly of Cowper's gland and prostate gland fluids as well as spermatozoa. The concentration of the high molecular weight proteinase inhibitors, alpha1-antitrypsin and alpha1X-antichymotrypsin, was essentially unaltered throughout the ejaculate fractions, although their absolute amounts showed an increase towards the final fraction. By contrast, the total inhibitory activity towards pancreatic trypsin was highest both in concentration and amount in the last fraction, thus indicating that the seminal vesicles are its primary source. Plasminogen, prothrombin, Factor XIII, and the proteinase inhibitors antithrombin III, alpha2-macroglobulin, inter-alpha-trypsin inhibitor and C1S-inactivator could not be detected immunochemically in whole ejaculates, and indicates the dissimilarity between the coagulation/liquefaction processes of semen and blood."}
{"id": "PMID:1083909", "title": "'Durate variant with clinical signs' has alpha1 -antitrypsin genotype ZZ.", "content": "A patient with neonatal jaundice and cirrhosis who was previously reported homozygous for the Durate variant of galactose-1-phosphate uridyl transferase has the ZZ genotype for alpha1-antitrypsin. A sister of the patient, also with ZZ genotype, is less severly affected with liver disease and is a heterozygote for the Durate variant. Since a number of patients with ZZ genotype of alpha1-antitrypsin have been previously reported to have liver disease, the latter genotype is the more probable explanation for the patients' clinical state. A question is raised, however, whether the Duarte variant may be specifically associated with the development of liver disease in ZZ individuals.", "contents": "'Durate variant with clinical signs' has alpha1 -antitrypsin genotype ZZ. A patient with neonatal jaundice and cirrhosis who was previously reported homozygous for the Durate variant of galactose-1-phosphate uridyl transferase has the ZZ genotype for alpha1-antitrypsin. A sister of the patient, also with ZZ genotype, is less severly affected with liver disease and is a heterozygote for the Durate variant. Since a number of patients with ZZ genotype of alpha1-antitrypsin have been previously reported to have liver disease, the latter genotype is the more probable explanation for the patients' clinical state. A question is raised, however, whether the Duarte variant may be specifically associated with the development of liver disease in ZZ individuals."}
{"id": "PMID:1083912", "title": "Effects of small organic molecules on the cholinergic receptors of the motor endplate.", "content": "This paper deals with the effects on the endplate receptors of the electro-osmotic and bath application of compounds which, although lacking pharmacological affinity, mimic the actions caused at much lower concentrations by conventional cholinergic drugs. Small cyclic molecules containing carbonyl, ether, ester, amide, and carboxyl anhydride groups were tested. Lactones, carboxyl anhydrides, cyclic amides, and ketones depolarized the endplate membrane and, in addition, most of them potentiated the depolarizing action of acetylcholine. On the contrary, cyclic ethers behaved as neuromuscular blocking agents. Dioxane, which contains two ether oxygens, caused a marked prolongation of the time course of the potentials elicited by the iontophoretic application of acetylcholine pulses. In some instances the effects of adding the compounds to the bath were different from those observed following local electro-osmotic application.", "contents": "Effects of small organic molecules on the cholinergic receptors of the motor endplate. This paper deals with the effects on the endplate receptors of the electro-osmotic and bath application of compounds which, although lacking pharmacological affinity, mimic the actions caused at much lower concentrations by conventional cholinergic drugs. Small cyclic molecules containing carbonyl, ether, ester, amide, and carboxyl anhydride groups were tested. Lactones, carboxyl anhydrides, cyclic amides, and ketones depolarized the endplate membrane and, in addition, most of them potentiated the depolarizing action of acetylcholine. On the contrary, cyclic ethers behaved as neuromuscular blocking agents. Dioxane, which contains two ether oxygens, caused a marked prolongation of the time course of the potentials elicited by the iontophoretic application of acetylcholine pulses. In some instances the effects of adding the compounds to the bath were different from those observed following local electro-osmotic application."}
{"id": "PMID:1083914", "title": "Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H.", "content": "The sites in Escherichia coli bacteriophage lambda DNA cleaved by the site-specific endonuclease isolated from Bacillus amyloliquefaciens H (BamI) are found to be at 0.114, 0.466, 0.580, 0.713, and 0.861 lambda units. The sites were located by analysis of the products of digestion of lambda DNA and lambda-ara transducing phage DNA, and verified by double digestion with BamI and EcoRI.", "contents": "Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H. The sites in Escherichia coli bacteriophage lambda DNA cleaved by the site-specific endonuclease isolated from Bacillus amyloliquefaciens H (BamI) are found to be at 0.114, 0.466, 0.580, 0.713, and 0.861 lambda units. The sites were located by analysis of the products of digestion of lambda DNA and lambda-ara transducing phage DNA, and verified by double digestion with BamI and EcoRI."}
{"id": "PMID:1083915", "title": "Prophage induction and inactivation by UV light.", "content": "Analysis of the induction curves for UV light-irradiated Haemophilus influenzae lysogens and the distribution of pyrimidine dimers in a repair-deficient lysogen suggests that one dimer per prophage-size segment of the host bacterial chromosome is necessary as a preinduction event. The close correlations obtained prompted a renewed consideration of the possibility that direct prophage induction occurs when one dimer is stabilized within the prophage genome. The host excision-repair system apparently functions to reduce the probability of \"stabilizing\" within the prophage those dimers that are necessary for induction and inactivation. The presence of the inducible defective prophage in strain Rd depresses the inducibility of prophage HP1c1.", "contents": "Prophage induction and inactivation by UV light. Analysis of the induction curves for UV light-irradiated Haemophilus influenzae lysogens and the distribution of pyrimidine dimers in a repair-deficient lysogen suggests that one dimer per prophage-size segment of the host bacterial chromosome is necessary as a preinduction event. The close correlations obtained prompted a renewed consideration of the possibility that direct prophage induction occurs when one dimer is stabilized within the prophage genome. The host excision-repair system apparently functions to reduce the probability of \"stabilizing\" within the prophage those dimers that are necessary for induction and inactivation. The presence of the inducible defective prophage in strain Rd depresses the inducibility of prophage HP1c1."}
{"id": "PMID:1083916", "title": "Immune reactivity in renal cancer: a sequential study.", "content": "We evaluated 18 patients with adenocarcinoma of the kidney immunologically in a linear fashion. Delayed cutaneous hypersensitivity was significantly altered in patients with metastatic disease. In vitro studies have shown a progressive decline in the absolute number of circulating lymphocytes and the T cell subpopulations that parallels the advance of the maligant process. Evaluation of lymphocyte function by the response to mitogens appears to be of little significance in a sequential study.", "contents": "Immune reactivity in renal cancer: a sequential study. We evaluated 18 patients with adenocarcinoma of the kidney immunologically in a linear fashion. Delayed cutaneous hypersensitivity was significantly altered in patients with metastatic disease. In vitro studies have shown a progressive decline in the absolute number of circulating lymphocytes and the T cell subpopulations that parallels the advance of the maligant process. Evaluation of lymphocyte function by the response to mitogens appears to be of little significance in a sequential study."}
{"id": "PMID:1083920", "title": "[Analysis of some parameters for determining myocardial contractility before and after aortocoronary shunting].", "content": "The condition of hemodynamics and the contractile function of the myocardium are considered before and after aorto-coronary shunting performed in patients with critically severe affection of the coronary arteries and the heart muscle. Of great prognostic importance is the final diastolic volume of the ventricles, its relation to the stroke ejection, as well as the ejection fraction. The greater the final diastolic volume of the ventricle and the smaller the stroke ejection, the higher is the risk of applying operative treatment to the patient.", "contents": "[Analysis of some parameters for determining myocardial contractility before and after aortocoronary shunting]. The condition of hemodynamics and the contractile function of the myocardium are considered before and after aorto-coronary shunting performed in patients with critically severe affection of the coronary arteries and the heart muscle. Of great prognostic importance is the final diastolic volume of the ventricles, its relation to the stroke ejection, as well as the ejection fraction. The greater the final diastolic volume of the ventricle and the smaller the stroke ejection, the higher is the risk of applying operative treatment to the patient."}
{"id": "PMID:1083922", "title": "Organ-specificity and diagnostic value of cell-mediated immunity against a liver-specific membrane protein: studies in hepatic and non-hepatic diseases.", "content": "In chronic active hepatitis (CAH, n=58) 70% of the HBsAg negative and 48% of the HBsAg positive cases showed a CMI against human liver specific proteins (HLPI). Using HBsAg as antigen only 12% of the HBsAg negative and 24% of the HBsAg positive cases gave a CMI response. On the basis of HBsAg and autoantibodies in the serum CAH patients could be divided into 4 subgroups. A close correlation between CMI against HLPI, sex, ANA and HL-A-8 could be detected. In a follow-up study of patients with acute virus B hepatitis (n=62) CMI against HBsAg was detected in 60% of the cases in the acute phase of the disease but in 15% only 3-6 months after the onset of the illness (n=40). In patients who developed a chronic HBsAg carrier status 3 of 5 cases remained persistently positive with HLPI as antigen in the migration inhibition test. - In non-hepatic diseases in which immunological abnormalities may be present (malignant diseases n=46, diabetes mellitus n=27, active tuberculosis, n=18 and untreated systemic lupus erythematodes, n=5) only 26% of patients with malignant diseases showed a migration inhibition with HLPI. - Using different antigens such as human liver specific proteins (HLP), rabbit liver specific proteins (RLP), brucella suis antigen and tuberculin it was possible to demonstrate the validity of the two-step migration inhibition test to detect CMI. The results with different antigens in hepatic and non-hepatic diseases demonstrated that cell-mediated immunity of HLPI is an organ specific immune reaction which is associated with acute and chronic active liver diseases as a time limited or long-lasting phenomenon. Positive reactions in some tumor patients suggest that different mechanisms may elicit an autoimmune reaction against liver antigens.", "contents": "Organ-specificity and diagnostic value of cell-mediated immunity against a liver-specific membrane protein: studies in hepatic and non-hepatic diseases. In chronic active hepatitis (CAH, n=58) 70% of the HBsAg negative and 48% of the HBsAg positive cases showed a CMI against human liver specific proteins (HLPI). Using HBsAg as antigen only 12% of the HBsAg negative and 24% of the HBsAg positive cases gave a CMI response. On the basis of HBsAg and autoantibodies in the serum CAH patients could be divided into 4 subgroups. A close correlation between CMI against HLPI, sex, ANA and HL-A-8 could be detected. In a follow-up study of patients with acute virus B hepatitis (n=62) CMI against HBsAg was detected in 60% of the cases in the acute phase of the disease but in 15% only 3-6 months after the onset of the illness (n=40). In patients who developed a chronic HBsAg carrier status 3 of 5 cases remained persistently positive with HLPI as antigen in the migration inhibition test. - In non-hepatic diseases in which immunological abnormalities may be present (malignant diseases n=46, diabetes mellitus n=27, active tuberculosis, n=18 and untreated systemic lupus erythematodes, n=5) only 26% of patients with malignant diseases showed a migration inhibition with HLPI. - Using different antigens such as human liver specific proteins (HLP), rabbit liver specific proteins (RLP), brucella suis antigen and tuberculin it was possible to demonstrate the validity of the two-step migration inhibition test to detect CMI. The results with different antigens in hepatic and non-hepatic diseases demonstrated that cell-mediated immunity of HLPI is an organ specific immune reaction which is associated with acute and chronic active liver diseases as a time limited or long-lasting phenomenon. Positive reactions in some tumor patients suggest that different mechanisms may elicit an autoimmune reaction against liver antigens."}
{"id": "PMID:1083923", "title": "[Use of specific anti-T-lymphocyte globulin (sATG) for the diagnosis of lymphoproliferative diseases (author's transl)].", "content": "Difficulties in the production of specific antisera against T-lymphocytes could be overcome by a stepwise absorption and purification procedure of anti-human thymocyte serum. Specific anti-T lymphocyte globulin (sATG) reacted with thymocytes, thymus-derived lymphocytes and a lymphoblastoid cell line of T-cell type whereas no activity was found against lymphoblastoid cell lines of B-cell type. Five chronic and three acute lymphatic leukemias were characterized using sATG in the cytotoxic test, electron microscopy, complement fixation test and quantitative immunoautoradiography, and compared with lymphocyte populations of normal individuals. Three chronic lymphatic leukemias with low numbers of spontaneous rosettes and high percentages of membrane-Ig-positive lymphocytes showed only few T-cell-antigen-positive lymphocytes and were therefore classified as B-cell leukemias. The cells of two chronic lymphatic leukemias with high numbers of spontaneous rosettes carried T-cell-antigen. The T-cell-antigen concentration, however, was lower than that of normal peripheral blood T-lymphocytes. The T-cell nature of two acute lymphatic leukemias with high numbers of spontaneous rosettes was confirmed by a positive reaction of the cells with sATG. In one case of acute lymphatic leukemia most leukemic cells carried T-cell-antigen although these cells did not form spontaneous rosettes. In the first two cases the T-cell-antigen concentration on the cell surface exceeded that of normal blood-T-lymphocytes, in the latter case it was slightly below that. The advantages of the characterization of leukemias with sATG in comparison with the spontaneous rosette formation and the relevance for prognosis are discussed.", "contents": "[Use of specific anti-T-lymphocyte globulin (sATG) for the diagnosis of lymphoproliferative diseases (author's transl)]. Difficulties in the production of specific antisera against T-lymphocytes could be overcome by a stepwise absorption and purification procedure of anti-human thymocyte serum. Specific anti-T lymphocyte globulin (sATG) reacted with thymocytes, thymus-derived lymphocytes and a lymphoblastoid cell line of T-cell type whereas no activity was found against lymphoblastoid cell lines of B-cell type. Five chronic and three acute lymphatic leukemias were characterized using sATG in the cytotoxic test, electron microscopy, complement fixation test and quantitative immunoautoradiography, and compared with lymphocyte populations of normal individuals. Three chronic lymphatic leukemias with low numbers of spontaneous rosettes and high percentages of membrane-Ig-positive lymphocytes showed only few T-cell-antigen-positive lymphocytes and were therefore classified as B-cell leukemias. The cells of two chronic lymphatic leukemias with high numbers of spontaneous rosettes carried T-cell-antigen. The T-cell-antigen concentration, however, was lower than that of normal peripheral blood T-lymphocytes. The T-cell nature of two acute lymphatic leukemias with high numbers of spontaneous rosettes was confirmed by a positive reaction of the cells with sATG. In one case of acute lymphatic leukemia most leukemic cells carried T-cell-antigen although these cells did not form spontaneous rosettes. In the first two cases the T-cell-antigen concentration on the cell surface exceeded that of normal blood-T-lymphocytes, in the latter case it was slightly below that. The advantages of the characterization of leukemias with sATG in comparison with the spontaneous rosette formation and the relevance for prognosis are discussed."}
{"id": "PMID:1083926", "title": "Valvulotomy of valves in the saphenous vein graft before coronary artery bypass.", "content": "Occasionally, repeat angiograms disclose turbulence, with or without clot formation, at the valve sites in saphenous veins used as coronary artery bypass grafts. A simple valvulotomy may be used to split these valves to allow a more even flow and to avoid the valve recess where air may be trapped or thrombus formed. A potential cause for perioperative infarction or graft failure may thus be avoided.", "contents": "Valvulotomy of valves in the saphenous vein graft before coronary artery bypass. Occasionally, repeat angiograms disclose turbulence, with or without clot formation, at the valve sites in saphenous veins used as coronary artery bypass grafts. A simple valvulotomy may be used to split these valves to allow a more even flow and to avoid the valve recess where air may be trapped or thrombus formed. A potential cause for perioperative infarction or graft failure may thus be avoided."}
{"id": "PMID:1083933", "title": "Upper gastrointestinal haemorrhage-a review of 100 cases.", "content": "The case records of the last 100 consecutive patients with upper gastrointestinal tract bleeding admitted to the adult section of the Mater Misericordiae Hospital have been analysed with attention to presentation, investigations, aetiology, management and mortality. The period of these admissions was almost three years-from May, 1971, to June, 1974.", "contents": "Upper gastrointestinal haemorrhage-a review of 100 cases. The case records of the last 100 consecutive patients with upper gastrointestinal tract bleeding admitted to the adult section of the Mater Misericordiae Hospital have been analysed with attention to presentation, investigations, aetiology, management and mortality. The period of these admissions was almost three years-from May, 1971, to June, 1974."}
{"id": "PMID:1083934", "title": "Arbovirus infection in a Murray Valley community. I. Prevalence of antibodies, December, 1974.", "content": "A survey of antibodies to Group A and Group B arboviruses (also designated as alphaviruses and flaviviruses respectively) was performed in residents of a Murray Valley town eight months after an outbreak of arbovirus illnesses in other parts of the region. The sampling pattern was based upon a random selection of households. The prevalence of Group A antibodies rose steadily with relation to age. The prevalence of Group B antibodies was much lower in all age groups, and did not show such a consistent rise with age. No Group B antibodies were found in younger residents. This suggests either an unexpectedly rapid decay of the particular type of antibody in this age group, or regional variations in the prevalence of arbovirus which are not currently understood. Some individuals appear to be unusually exposed to arbovirus infection.", "contents": "Arbovirus infection in a Murray Valley community. I. Prevalence of antibodies, December, 1974. A survey of antibodies to Group A and Group B arboviruses (also designated as alphaviruses and flaviviruses respectively) was performed in residents of a Murray Valley town eight months after an outbreak of arbovirus illnesses in other parts of the region. The sampling pattern was based upon a random selection of households. The prevalence of Group A antibodies rose steadily with relation to age. The prevalence of Group B antibodies was much lower in all age groups, and did not show such a consistent rise with age. No Group B antibodies were found in younger residents. This suggests either an unexpectedly rapid decay of the particular type of antibody in this age group, or regional variations in the prevalence of arbovirus which are not currently understood. Some individuals appear to be unusually exposed to arbovirus infection."}
{"id": "PMID:1083938", "title": "Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases.", "content": "Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcription units. They are superimposed on a rather broad smear of background DNA, representing the heterogenous 'spacer' sequences. From the restriction maps, together with data obtained by partial digestion, a physical map for the ribosomal transcription unit in yeast could be constructed.", "contents": "Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcription units. They are superimposed on a rather broad smear of background DNA, representing the heterogenous 'spacer' sequences. From the restriction maps, together with data obtained by partial digestion, a physical map for the ribosomal transcription unit in yeast could be constructed."}
{"id": "PMID:1083961", "title": "E rosettes in lymphoproliferative diseases.", "content": "Results are given of investigations of E rosettes in patients with chronic lymphadenosis and malignant lymphoma. The quantity of E rosettes was expressed both as per cents and in absolute numbers calculated from the total amount of lymphocytes in peripheral blood. In the normal controls the percentage of E rosettes was 58% on average while in patients with chronic lymphadenosis it was found to have decreased significantly down to a mean of 6%. However, in absolute values the number of rosette forming lymphocytes was normal and even higher. In the group with malignant lymphoma the percentage decrease was less striking than in those with chronic lymphadenosis, however, the absolute number of rosette forming lymphocytes was always found to be lowered because of evident to considerable lymphopenia. A dynamic study of the observed changes might contribute to a knowledge of the pathophysiology of lymphoproliferative diseases.", "contents": "E rosettes in lymphoproliferative diseases. Results are given of investigations of E rosettes in patients with chronic lymphadenosis and malignant lymphoma. The quantity of E rosettes was expressed both as per cents and in absolute numbers calculated from the total amount of lymphocytes in peripheral blood. In the normal controls the percentage of E rosettes was 58% on average while in patients with chronic lymphadenosis it was found to have decreased significantly down to a mean of 6%. However, in absolute values the number of rosette forming lymphocytes was normal and even higher. In the group with malignant lymphoma the percentage decrease was less striking than in those with chronic lymphadenosis, however, the absolute number of rosette forming lymphocytes was always found to be lowered because of evident to considerable lymphopenia. A dynamic study of the observed changes might contribute to a knowledge of the pathophysiology of lymphoproliferative diseases."}
{"id": "PMID:1083962", "title": "The effect of apomorphine and ergocriptine on the release of MSH by the pars intermedia of Rana pipiens.", "content": "In an attempt to further characterize the nature of the innervation controlling melanophore-stimulating hormone (MSH) release from the pars intermedia (PI), pellets containing either apomorphine or ergocriptine were implanted in contact with the PI of the frog, and the effect on the animal's coloration was observed. Frogs with apomorphine implants exhibited skin pallor for several hours and those with ergocriptine implants were pallid for a few days. Hypophysectomized (HX) frogs which had been darkened with MSH regained their pallor no sooner when given an injection of apomorphine than with the vehicle. However, intact dark frogs lightened when injected with apomorphine. Frogs darkened by autotransplantation of the PI to the dorsal lymph sac were lightened by an injection of apomorphine. Frogs lightened by an ergocriptine implant on the PI and then darkened by MSH did not lighten sooner than HX frogs given an injection of MSH. Ergocriptine-containing pellets implanted in the thigh took longer to produce the same effect than those implanted in contact with the PI. Since apomorphine is known to stimulate dopamine receptors in the central nervous system, and there is some evidence that ergocriptine does also, the above evidence supports the hypothesis that dopamine receptors are present in the PI.", "contents": "The effect of apomorphine and ergocriptine on the release of MSH by the pars intermedia of Rana pipiens. In an attempt to further characterize the nature of the innervation controlling melanophore-stimulating hormone (MSH) release from the pars intermedia (PI), pellets containing either apomorphine or ergocriptine were implanted in contact with the PI of the frog, and the effect on the animal's coloration was observed. Frogs with apomorphine implants exhibited skin pallor for several hours and those with ergocriptine implants were pallid for a few days. Hypophysectomized (HX) frogs which had been darkened with MSH regained their pallor no sooner when given an injection of apomorphine than with the vehicle. However, intact dark frogs lightened when injected with apomorphine. Frogs darkened by autotransplantation of the PI to the dorsal lymph sac were lightened by an injection of apomorphine. Frogs lightened by an ergocriptine implant on the PI and then darkened by MSH did not lighten sooner than HX frogs given an injection of MSH. Ergocriptine-containing pellets implanted in the thigh took longer to produce the same effect than those implanted in contact with the PI. Since apomorphine is known to stimulate dopamine receptors in the central nervous system, and there is some evidence that ergocriptine does also, the above evidence supports the hypothesis that dopamine receptors are present in the PI."}
{"id": "PMID:1083966", "title": "Disseminated intravascular coagulation and fibrinolysis as a cause of postextraction hemorrhage. Report of a case.", "content": "Presented is a case in which severe, prolonged hemorrhage, subsequent to simple dental extractions in an 85-year-old Negro man, was due to disseminated intravascular coagulation, with secondary fibrinolysis resulting from metastatic adenocarcinoma of the prostate gland. The difficulty and delay in diagnosis, selection of proper therapeutic regimen, and complications encountered during treatment are discussed.", "contents": "Disseminated intravascular coagulation and fibrinolysis as a cause of postextraction hemorrhage. Report of a case. Presented is a case in which severe, prolonged hemorrhage, subsequent to simple dental extractions in an 85-year-old Negro man, was due to disseminated intravascular coagulation, with secondary fibrinolysis resulting from metastatic adenocarcinoma of the prostate gland. The difficulty and delay in diagnosis, selection of proper therapeutic regimen, and complications encountered during treatment are discussed."}
{"id": "PMID:1083972", "title": "Voltage clamp analysis in isolated cardiac fibres as performed with two different perfusion chambres for double sucrose gap.", "content": "Voltage clamp experiments were performed on isolated frog atrial trabeculae disposed in 2 different perfusion chambres for double sucrose gap. In one of the perfusion chambres, a \"liquid partition system\" (L.P.S.) was used; in the other, a vaseline sealing (V.P.S.) method was used to separate the various fluid compartments. From the linear electrical properties, the elements of an apparent equivalent electrical circuit were calculated. The apparent nodal capacity was significantly larger in fibres disposed in the \"liquid partition system\" than those disposed in the \"vaseline partition system\". The apparent \"error factor\" resulting from the presence of the series resistance was larger in the L.P.S. than in the V.P.S. The apparent \"error factor\" becomes relatively large when the membrane conductance increases, such as during the flow of the inward current. A rapid desactivation of the peak inward current was found on bringing the command potential back to the resting potential in the V.P.S. This was not found in the L.P.S., indicating better voltage control with the V.P.S. than with the L.P.S. Transmembrane microelectrode recordings during voltage clamp experiment in the V.P.S. indicated satisfactory voltage control during the flow of the peak inward current. Adequate voltage control is lost when notches or irregularities appear on the current traces.", "contents": "Voltage clamp analysis in isolated cardiac fibres as performed with two different perfusion chambres for double sucrose gap. Voltage clamp experiments were performed on isolated frog atrial trabeculae disposed in 2 different perfusion chambres for double sucrose gap. In one of the perfusion chambres, a \"liquid partition system\" (L.P.S.) was used; in the other, a vaseline sealing (V.P.S.) method was used to separate the various fluid compartments. From the linear electrical properties, the elements of an apparent equivalent electrical circuit were calculated. The apparent nodal capacity was significantly larger in fibres disposed in the \"liquid partition system\" than those disposed in the \"vaseline partition system\". The apparent \"error factor\" resulting from the presence of the series resistance was larger in the L.P.S. than in the V.P.S. The apparent \"error factor\" becomes relatively large when the membrane conductance increases, such as during the flow of the inward current. A rapid desactivation of the peak inward current was found on bringing the command potential back to the resting potential in the V.P.S. This was not found in the L.P.S., indicating better voltage control with the V.P.S. than with the L.P.S. Transmembrane microelectrode recordings during voltage clamp experiment in the V.P.S. indicated satisfactory voltage control during the flow of the peak inward current. Adequate voltage control is lost when notches or irregularities appear on the current traces."}
{"id": "PMID:1083978", "title": "[Early functional disorders in 3 children exhibiting an obstructive syndrome and an alpha 1 antitrypsin deficiency].", "content": "The respiratory function is studied in 3 children, two of which 4 years in age, exhibiting a respiratory obstructive syndrome and an alpha1-antitrypsin deficiency. The study of the respiratory function is global and regional. The disorders noted include a ventilatory asynchronism, a distension, regional anomalies of ventilation and perfusion. The ventilatory asynchronism appears to be an early disorder in the course of alpha1-antitrypsin deficiency. These results have to be related with the development of the elastic fibers during the child's growth.", "contents": "[Early functional disorders in 3 children exhibiting an obstructive syndrome and an alpha 1 antitrypsin deficiency]. The respiratory function is studied in 3 children, two of which 4 years in age, exhibiting a respiratory obstructive syndrome and an alpha1-antitrypsin deficiency. The study of the respiratory function is global and regional. The disorders noted include a ventilatory asynchronism, a distension, regional anomalies of ventilation and perfusion. The ventilatory asynchronism appears to be an early disorder in the course of alpha1-antitrypsin deficiency. These results have to be related with the development of the elastic fibers during the child's growth."}
{"id": "PMID:1083979", "title": "Separation of inner and outer membranes of Rhodopseudomonas spheroides.", "content": "The separation of inner and outer membrane of Rhodopseudomonas spheroides has been achieved by means of sucrose density gradient (20%, 40%, 60%, w/w) centrifugation. The upper fraction of the gradient, with a specific density 1.181 (g/cm3), is high in cytochrome and succinate dehydrogenase activities, low in lipopolysaccharides and it is designated the inner membrane fraction. The bottom fraction of the gradient, with a specific density 1.240, is high in lipopolysaccharide and contains neither cytochrome nor succinate dehydrogenase activities. This fraction is the cell wall or outer membrane fraction. The intermediate band on the gradient is an unseparated fraction of inner and outer membrane fragments. This fraction has a specific denisty of 1.211 and represents less than 3% of total crude envelope. Thin sections of the vesicles of the inner membrane fraction and those of outer membrane provide morphological evidence for the identity of the individual membrane fractions. At least 22 protein bands are resolved by employing sodium dodecyl sulfate slab gel electrophoresis. Six bands are present only in the inner membrane and two bands are found exclusively in the outer membrane. Most of the remaining polypeptides are present in greater amounts in the inner membrane relative to the outer membrane fractions.", "contents": "Separation of inner and outer membranes of Rhodopseudomonas spheroides. The separation of inner and outer membrane of Rhodopseudomonas spheroides has been achieved by means of sucrose density gradient (20%, 40%, 60%, w/w) centrifugation. The upper fraction of the gradient, with a specific density 1.181 (g/cm3), is high in cytochrome and succinate dehydrogenase activities, low in lipopolysaccharides and it is designated the inner membrane fraction. The bottom fraction of the gradient, with a specific density 1.240, is high in lipopolysaccharide and contains neither cytochrome nor succinate dehydrogenase activities. This fraction is the cell wall or outer membrane fraction. The intermediate band on the gradient is an unseparated fraction of inner and outer membrane fragments. This fraction has a specific denisty of 1.211 and represents less than 3% of total crude envelope. Thin sections of the vesicles of the inner membrane fraction and those of outer membrane provide morphological evidence for the identity of the individual membrane fractions. At least 22 protein bands are resolved by employing sodium dodecyl sulfate slab gel electrophoresis. Six bands are present only in the inner membrane and two bands are found exclusively in the outer membrane. Most of the remaining polypeptides are present in greater amounts in the inner membrane relative to the outer membrane fractions."}
{"id": "PMID:1083980", "title": "[Biosynthesis of 5- methoxy-N-acetyltryptamine (melatonin) in enterochromaffin cells].", "content": "Chromatographic analysis of the alkaline extract of the gastrointestinal mucosa of rabbits rich in enterochromaffin cells displayed the presence of melatonin (5-methoxy-N-acetyltryptamine) and its precursors (5-oxytryptophane, tryptamine, serotonine, mexamine) in it. Detection of precursors indicated that not a passive accumulation, but an active synthesis of melatonin occurred in the enterochromaffin cells. Epiphysis was not the only place of melatonin synthesis. Functional condition of enterochromaffin cells was largely determined by the extent of physiological and pathological reactions of the organism.", "contents": "[Biosynthesis of 5- methoxy-N-acetyltryptamine (melatonin) in enterochromaffin cells]. Chromatographic analysis of the alkaline extract of the gastrointestinal mucosa of rabbits rich in enterochromaffin cells displayed the presence of melatonin (5-methoxy-N-acetyltryptamine) and its precursors (5-oxytryptophane, tryptamine, serotonine, mexamine) in it. Detection of precursors indicated that not a passive accumulation, but an active synthesis of melatonin occurred in the enterochromaffin cells. Epiphysis was not the only place of melatonin synthesis. Functional condition of enterochromaffin cells was largely determined by the extent of physiological and pathological reactions of the organism."}
{"id": "PMID:1083981", "title": "Antibody-producing cells: virus-induced alteration of response to antigen.", "content": "Spleen antibody-forming cells of mice yield a 3- to 10-fold increase in their response to sheep erythrocyte antigen if they are acutely infected by lactate dehydrogenase-elevating virus. This early stimulation is replaced by a long-term inhibition of the antibody-forming cells as the viremia goes into its persisting chronic stage. These contrasting immunological phenomena are examined as contributing factors responsible for the enhancement by this virus of asparaginase (EC 3.5.1.1; L-asparagine amidolydrolase) therapy against leukemia in mice, and for the alteration of the susceptibility of mice to various neoplastic processes.", "contents": "Antibody-producing cells: virus-induced alteration of response to antigen. Spleen antibody-forming cells of mice yield a 3- to 10-fold increase in their response to sheep erythrocyte antigen if they are acutely infected by lactate dehydrogenase-elevating virus. This early stimulation is replaced by a long-term inhibition of the antibody-forming cells as the viremia goes into its persisting chronic stage. These contrasting immunological phenomena are examined as contributing factors responsible for the enhancement by this virus of asparaginase (EC 3.5.1.1; L-asparagine amidolydrolase) therapy against leukemia in mice, and for the alteration of the susceptibility of mice to various neoplastic processes."}
{"id": "PMID:1084002", "title": "[Electrophoretic mobility of human lymphocytes purified by nylon columns and spontaneous rosettes].", "content": "Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes.", "contents": "[Electrophoretic mobility of human lymphocytes purified by nylon columns and spontaneous rosettes]. Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes."}
{"id": "PMID:1084006", "title": "[Xeroradiography in rheumatology].", "content": "Xeroradiography is a new and simple radiodiagnostic technique that makes it possible to analyse on one positive film not only the bone tissue but also the neighbouring structures (ligaments, muscles, fatty tissue, the vessels, and the skin). On the basis of about 100 examinations, the authors indicate the value and the limitations of the technique in rheumatological practice. Because of the technical aspects of the technique, the dorsolumbar spine and the facial massif are poorly visualized. The swamping of the contrast also limits its use for cases of demineralization (osteoporosis). On the other hand, the information provided by the technique in cases of Paget's disease, articular or para-articular calcification, pathological conditions of the tendons (rupture, calcification), and bone tumours appears to be of great value. The early diagnosis of cases of inflammatory rheumatism and the surveillance of Silastic prostheses used in surgery on rheumatic hands are also facilitated.", "contents": "[Xeroradiography in rheumatology]. Xeroradiography is a new and simple radiodiagnostic technique that makes it possible to analyse on one positive film not only the bone tissue but also the neighbouring structures (ligaments, muscles, fatty tissue, the vessels, and the skin). On the basis of about 100 examinations, the authors indicate the value and the limitations of the technique in rheumatological practice. Because of the technical aspects of the technique, the dorsolumbar spine and the facial massif are poorly visualized. The swamping of the contrast also limits its use for cases of demineralization (osteoporosis). On the other hand, the information provided by the technique in cases of Paget's disease, articular or para-articular calcification, pathological conditions of the tendons (rupture, calcification), and bone tumours appears to be of great value. The early diagnosis of cases of inflammatory rheumatism and the surveillance of Silastic prostheses used in surgery on rheumatic hands are also facilitated."}
{"id": "PMID:1084007", "title": "[Osteoid osteoma. 5 cases].", "content": "With reference to five cases of osteoid osteoma, including two para-articular cases with articular reaction, two cases with neurological manifestations, and one case with a rare costal lesion, the authors demonstrate the difficulty of diagnosis in this disease, which is, in principle, well known. In some cases the observations extended over seven years. The authors also review the literature on this subject.", "contents": "[Osteoid osteoma. 5 cases]. With reference to five cases of osteoid osteoma, including two para-articular cases with articular reaction, two cases with neurological manifestations, and one case with a rare costal lesion, the authors demonstrate the difficulty of diagnosis in this disease, which is, in principle, well known. In some cases the observations extended over seven years. The authors also review the literature on this subject."}
{"id": "PMID:1084008", "title": "[New research on synovial enzymology].", "content": "After reviewing previous work on the subjects, the authors show that the synovial fluid in subjects with inflammatory rheumatism contained higher levels of lysozyme and of beta-glucuronidase comparable with those of the acid phosphatases and of lacto-dehydrogenase that they were interested in previously.", "contents": "[New research on synovial enzymology]. After reviewing previous work on the subjects, the authors show that the synovial fluid in subjects with inflammatory rheumatism contained higher levels of lysozyme and of beta-glucuronidase comparable with those of the acid phosphatases and of lacto-dehydrogenase that they were interested in previously."}
{"id": "PMID:1084009", "title": "[Rheumatic manifestations in 80 cases of Crohn's disease].", "content": "Out of a series of 80 patients suffering from Crohn's disease, 31 presented rheumatic manifestations. In 16 subjects this took the form of synovitis closely dependent on the enteritic evolution, which developed after the alimentary symptoms, and which worsened as they did and sometimes regressed as they did following medical or surgical treatment. In combination with erythema nodosum, aphtosis, and conjunctivitis, synovitis appears to be the expression of an immune response to the enteritic lesion. Three cases of chronic polyarthitis and 6 cases of asymptomatic sacro-ileitis were also observed, and 6 cases of spondylarthritis of a minor radiological type were observed that evolved independently of the Crohn's disease. Typing according to the HLA system using 26 antigens was carried out in 44 subjects; no difference in phenotype frequency was found between a control group (blood donors) and the group of subjects with Crohn's disease alone; however, the antigen W 17 was found significantly more frequently in those subjects with peripheral arthritis and the antigen W 27 was found more frequently in those with spondylarthritis. These findings suggest, although it is not certain, the existence of genetic susceptibility to rheumatic manifestations in certain sites in patients with Crohn's disease.", "contents": "[Rheumatic manifestations in 80 cases of Crohn's disease]. Out of a series of 80 patients suffering from Crohn's disease, 31 presented rheumatic manifestations. In 16 subjects this took the form of synovitis closely dependent on the enteritic evolution, which developed after the alimentary symptoms, and which worsened as they did and sometimes regressed as they did following medical or surgical treatment. In combination with erythema nodosum, aphtosis, and conjunctivitis, synovitis appears to be the expression of an immune response to the enteritic lesion. Three cases of chronic polyarthitis and 6 cases of asymptomatic sacro-ileitis were also observed, and 6 cases of spondylarthritis of a minor radiological type were observed that evolved independently of the Crohn's disease. Typing according to the HLA system using 26 antigens was carried out in 44 subjects; no difference in phenotype frequency was found between a control group (blood donors) and the group of subjects with Crohn's disease alone; however, the antigen W 17 was found significantly more frequently in those subjects with peripheral arthritis and the antigen W 27 was found more frequently in those with spondylarthritis. These findings suggest, although it is not certain, the existence of genetic susceptibility to rheumatic manifestations in certain sites in patients with Crohn's disease."}
{"id": "PMID:1084010", "title": "[Pigmented villonodular synovitits of the hip: ultrastructure and aspects on scanning electron microscopy].", "content": "The authors studied one case of pigmented, villonodular synovitis (PVNS) of the hip by means of optical microscopy and by transmission and scanning electron microscopy. Scanning electron microscopy showed that the surface of the PVNS is completely different from that of rheumatic synovitis, in particular that of rheumatoid synovitis. The composition and the cellular morphology of the outer layer of the PVNS appear, however, to be similar to those of normal synovial membrane of arthrosic synovial membrane. Clumps of red corpuscles, enclosed in a fibrin network, were visible on the surface of the PVNS in a way that the authors has never seen previously in the 19 other human synovial membranes, normal and pathological, they had studied in this way. The totality of the microscopic findings confirms the importance of the role played by the intra-articular and intra-synovial haemorrhages, and by the macrophage reactions that follow, in the development of the lesions that characterize PVNS.", "contents": "[Pigmented villonodular synovitits of the hip: ultrastructure and aspects on scanning electron microscopy]. The authors studied one case of pigmented, villonodular synovitis (PVNS) of the hip by means of optical microscopy and by transmission and scanning electron microscopy. Scanning electron microscopy showed that the surface of the PVNS is completely different from that of rheumatic synovitis, in particular that of rheumatoid synovitis. The composition and the cellular morphology of the outer layer of the PVNS appear, however, to be similar to those of normal synovial membrane of arthrosic synovial membrane. Clumps of red corpuscles, enclosed in a fibrin network, were visible on the surface of the PVNS in a way that the authors has never seen previously in the 19 other human synovial membranes, normal and pathological, they had studied in this way. The totality of the microscopic findings confirms the importance of the role played by the intra-articular and intra-synovial haemorrhages, and by the macrophage reactions that follow, in the development of the lesions that characterize PVNS."}
{"id": "PMID:1084013", "title": "Evidence that carrier-specific B cells play a role in cellular collaboration.", "content": "Evidence is presented showing that carrier-specific B cells play a significant role in inducing an anti-hapten antibody response to the thymus-dependent antigen, dinitrophenylated monomeric flagellin (DNP-MON), both in vivo and in vitro. Inactivation of carrier-specific B cells by (125I) polymeric flagellin (POL) suicide decreased the anti-DNP IgM response by 40%-50% and reduced the anti-DNP IgG response by 85%-90%. Several lines of evidence established that the suicide procedure eliminated carrier-specific B cells rather than carrier-specific T (helper) cells.", "contents": "Evidence that carrier-specific B cells play a role in cellular collaboration. Evidence is presented showing that carrier-specific B cells play a significant role in inducing an anti-hapten antibody response to the thymus-dependent antigen, dinitrophenylated monomeric flagellin (DNP-MON), both in vivo and in vitro. Inactivation of carrier-specific B cells by (125I) polymeric flagellin (POL) suicide decreased the anti-DNP IgM response by 40%-50% and reduced the anti-DNP IgG response by 85%-90%. Several lines of evidence established that the suicide procedure eliminated carrier-specific B cells rather than carrier-specific T (helper) cells."}
{"id": "PMID:1084014", "title": "The effect of anti-immunoglobulin antibodies of Fc rosettes.", "content": "This study demonstrated that Fc rosettes detected by antibody-labeled bovine erythrocytes can be inhibited by immune complexes of rabbit IgG (b4 allotype) and anti-b4 IgG, thus showing that these complexes effectively compete for the receptor for erythrocyte-antibody rosettes. Incubation of mouse lymphocytes with goat F(ab')2 anti-mouse IgG did not inhibit Fc rosettes. When immunoglobulin on murine and human B lymphocytes was redistributed in a polar cap on the membrane by interaction with anti-immunoglobulin antibodies, the number of Fc rosettes after 'capping' was unchanged; most of the indicator erythrocytes, however, were distributed in a cap as well. This erythrocyte cap was superimposed on the immunoglobulin cap.", "contents": "The effect of anti-immunoglobulin antibodies of Fc rosettes. This study demonstrated that Fc rosettes detected by antibody-labeled bovine erythrocytes can be inhibited by immune complexes of rabbit IgG (b4 allotype) and anti-b4 IgG, thus showing that these complexes effectively compete for the receptor for erythrocyte-antibody rosettes. Incubation of mouse lymphocytes with goat F(ab')2 anti-mouse IgG did not inhibit Fc rosettes. When immunoglobulin on murine and human B lymphocytes was redistributed in a polar cap on the membrane by interaction with anti-immunoglobulin antibodies, the number of Fc rosettes after 'capping' was unchanged; most of the indicator erythrocytes, however, were distributed in a cap as well. This erythrocyte cap was superimposed on the immunoglobulin cap."}
{"id": "PMID:1084015", "title": "The role of B-cell memory in secondary IgG and IgM responses.", "content": "Mice were primed with the hapten 3-nitro-4-hydroxy-5 iodophenacetic acid (NIP) conjugated to chicken globulin (cg) and were boosted 2, 6, or 12 months later with CG conjugates of the related haptens 3,5-diiodo-4-hydroxyphenacetic acid (DIP) or 3-nitro-4-hydroxphenacetic acid (NP). Accelerated secondary responses were demonstrated both in the 7S and 19S class. Fine-specificities of secondary-response antibodies were studied by the hapten inhibition method of haptenated bacteriophage inactivation. 7S antibodies were found to have the fine-specificity of anti-NIP antibodies regardless of whether DIP or NP was the booster hapten ('original antigenic sin'). 19S antibodies had the fine-specificity of anti-DIP when DIP was the booster hapten. NP as the booster hapten resulted in 19S antibodies whose fine-specificity was intermediate between anti-NIP and anti-NP. A strong B-cell memory could thus be demonstrated in the 7S antibody response and a weak B-cell memory in the 19S antibody response.", "contents": "The role of B-cell memory in secondary IgG and IgM responses. Mice were primed with the hapten 3-nitro-4-hydroxy-5 iodophenacetic acid (NIP) conjugated to chicken globulin (cg) and were boosted 2, 6, or 12 months later with CG conjugates of the related haptens 3,5-diiodo-4-hydroxyphenacetic acid (DIP) or 3-nitro-4-hydroxphenacetic acid (NP). Accelerated secondary responses were demonstrated both in the 7S and 19S class. Fine-specificities of secondary-response antibodies were studied by the hapten inhibition method of haptenated bacteriophage inactivation. 7S antibodies were found to have the fine-specificity of anti-NIP antibodies regardless of whether DIP or NP was the booster hapten ('original antigenic sin'). 19S antibodies had the fine-specificity of anti-DIP when DIP was the booster hapten. NP as the booster hapten resulted in 19S antibodies whose fine-specificity was intermediate between anti-NIP and anti-NP. A strong B-cell memory could thus be demonstrated in the 7S antibody response and a weak B-cell memory in the 19S antibody response."}
{"id": "PMID:1084016", "title": "Distribution of cells binding erythrocyte-antibody (EA) complexes in human lymphoid populations.", "content": "Cells bearing Fc receptors were studied using a rosette technique with chicken erythrocytes coated with rabbit antibodies (EA). By comparison with other markers and by selective depletion of T and B cells it was demonstrated that EA rosettes were formed by about 10% of the monocytes, 7% of the T lymphocytes, and 45% of the B cells. In addition, 26% of EA-rosette-forming cells (EA-RFC) in the peripheral blood carried on other markers than C3 receptors. Marked differences were found in the percentages of EA-RFC in cell suspensions from various lymphoid organs: peripheral blood, 15.2%; spleen, 26.6%; bone marrow, 30.9%; lymph nodes, 2.8%; thymus, 0.3%; and tonsils, 0.5%. It was concluded that Fc receptors were present on subsets of different lymphocyte subpopulations; their presence could not be regarded as a specific marker of one of these subpopulations.", "contents": "Distribution of cells binding erythrocyte-antibody (EA) complexes in human lymphoid populations. Cells bearing Fc receptors were studied using a rosette technique with chicken erythrocytes coated with rabbit antibodies (EA). By comparison with other markers and by selective depletion of T and B cells it was demonstrated that EA rosettes were formed by about 10% of the monocytes, 7% of the T lymphocytes, and 45% of the B cells. In addition, 26% of EA-rosette-forming cells (EA-RFC) in the peripheral blood carried on other markers than C3 receptors. Marked differences were found in the percentages of EA-RFC in cell suspensions from various lymphoid organs: peripheral blood, 15.2%; spleen, 26.6%; bone marrow, 30.9%; lymph nodes, 2.8%; thymus, 0.3%; and tonsils, 0.5%. It was concluded that Fc receptors were present on subsets of different lymphocyte subpopulations; their presence could not be regarded as a specific marker of one of these subpopulations."}
{"id": "PMID:1084017", "title": "Antibody-dependent cell cytoxicity (ADCC). Characterization of 'killer' cells in human lymphoid organs.", "content": "The effector cell(s) in human antibody-dependent cell cytotoxicity (ADCC), with antibody-coated chickens erythrocytes as targets, was studied by comparison of cell suspensions from various lymphoid organs and by means of various cell fractionation methods. Effector cells (K) were found mostly in peripheral blood, spleen, and bone marrow but not in tonsils, lymph nodes, and thymus. Effector cells bear Fc receptors and can form EA rosettes with the antibody-coated target cells. About 1,5% peripheral blood lymphocytes can form 'high-avidity' EA rosettes with targets coated at low antiserum concentration. Most of the effector cells belong to this small subset, as shown by experiments of selective depletion. Removal of most monocytes, T cells, or B cells from, or addition of T-cell-specific antiserum to, the effector cell suspensions did not affect ADCC. Effector cells in this model of ADCC therefore lack the conventional B- or T-cell markers but at least some of them are likely to bear C3 receptors.", "contents": "Antibody-dependent cell cytoxicity (ADCC). Characterization of 'killer' cells in human lymphoid organs. The effector cell(s) in human antibody-dependent cell cytotoxicity (ADCC), with antibody-coated chickens erythrocytes as targets, was studied by comparison of cell suspensions from various lymphoid organs and by means of various cell fractionation methods. Effector cells (K) were found mostly in peripheral blood, spleen, and bone marrow but not in tonsils, lymph nodes, and thymus. Effector cells bear Fc receptors and can form EA rosettes with the antibody-coated target cells. About 1,5% peripheral blood lymphocytes can form 'high-avidity' EA rosettes with targets coated at low antiserum concentration. Most of the effector cells belong to this small subset, as shown by experiments of selective depletion. Removal of most monocytes, T cells, or B cells from, or addition of T-cell-specific antiserum to, the effector cell suspensions did not affect ADCC. Effector cells in this model of ADCC therefore lack the conventional B- or T-cell markers but at least some of them are likely to bear C3 receptors."}
{"id": "PMID:1084018", "title": "Does nonspecific T-lymphocyte stimulation of B lymphocytes occur during reversal reaction in borderline leprosy?", "content": "Serum immunoglobulins G, A, and M were estimated in 14 patients with border-line cases of leprosy at commencement of treatment and subsequently when they developed 'reversal reaction'. There was a significant increase in all immunoglobulin levels during the reaction, with a subsequent fall; the postreaction values for IgG and IgA were below the base-line figures. Additonal investigations in six patients indicated that the rise was a nonspecific one, not brought about by an increase in antimycobacterial antibodies. It seems likely that the rise in immunoglobulins during reaction is due to nonspecific T-lymphocyte stimulation of B lymphocytes.", "contents": "Does nonspecific T-lymphocyte stimulation of B lymphocytes occur during reversal reaction in borderline leprosy? Serum immunoglobulins G, A, and M were estimated in 14 patients with border-line cases of leprosy at commencement of treatment and subsequently when they developed 'reversal reaction'. There was a significant increase in all immunoglobulin levels during the reaction, with a subsequent fall; the postreaction values for IgG and IgA were below the base-line figures. Additonal investigations in six patients indicated that the rise was a nonspecific one, not brought about by an increase in antimycobacterial antibodies. It seems likely that the rise in immunoglobulins during reaction is due to nonspecific T-lymphocyte stimulation of B lymphocytes."}
{"id": "PMID:1084020", "title": "Endoscopic removal of gastric and duodenal polyps.", "content": "As endoscopic surgery comes into its own, the applications of this modality are increasing. This is well illustrated by the use of endoscopic polypectomy in the colon, and more recently by endoscopic polypectomy in the stomach and duodenum. We can anticipate increased applicability of these technics as the experience widens and the margins of safety increase. The present series of six polypectomies from the stomach and duodenum confirms the applicability of endoscopic polypectomy for this portion of the gastrointestinal tract, attests to its safety, and indicates that these procedures can contribute materially to the care of patients with gastric or duodenal polyps. The real and potential problems are discussed.", "contents": "Endoscopic removal of gastric and duodenal polyps. As endoscopic surgery comes into its own, the applications of this modality are increasing. This is well illustrated by the use of endoscopic polypectomy in the colon, and more recently by endoscopic polypectomy in the stomach and duodenum. We can anticipate increased applicability of these technics as the experience widens and the margins of safety increase. The present series of six polypectomies from the stomach and duodenum confirms the applicability of endoscopic polypectomy for this portion of the gastrointestinal tract, attests to its safety, and indicates that these procedures can contribute materially to the care of patients with gastric or duodenal polyps. The real and potential problems are discussed."}
{"id": "PMID:1084021", "title": "Coagulation studies in leptospirosis.", "content": "Coagulation and serum F.D.P. studies in 10 leptospirosis patients (5, L. bataviae, 2, L. autumnalis, 2, L. australis, and 1, L. akiyami A.)showed prolongation of prothrombin time, partial thromboplastin time and thrombin clotting time, slight low platelet count, depletion of factor V and slightly high serum F.D.P. in only four cases. Four cases had bleeding and one case died because of severe haemorrhage but with only slight changes in coagulation factors and slightly high serum F.D.P. These findings suggested that the damage of capillary endothelium was more pronounced than the coagulopathy which may be due to liver cell damage.", "contents": "Coagulation studies in leptospirosis. Coagulation and serum F.D.P. studies in 10 leptospirosis patients (5, L. bataviae, 2, L. autumnalis, 2, L. australis, and 1, L. akiyami A.)showed prolongation of prothrombin time, partial thromboplastin time and thrombin clotting time, slight low platelet count, depletion of factor V and slightly high serum F.D.P. in only four cases. Four cases had bleeding and one case died because of severe haemorrhage but with only slight changes in coagulation factors and slightly high serum F.D.P. These findings suggested that the damage of capillary endothelium was more pronounced than the coagulopathy which may be due to liver cell damage."}
{"id": "PMID:1084022", "title": "A bleeding syndrome in infants: acquired prothrombin complex deficiency of unknown aetiology.", "content": "A total of 240 cases of a bleeding syndrome in infants due to prothrombin complex deficiency of unknown aetiology were reviewed. The majority of patients were breast fed, aged 1-2 months and the syndrome was more prevalent in males. Clinical manifestations consisted of bleeding, pallor and mild hepatomegaly in the majority of cases. Mild fever, diarrhoea, jaundice, and upper respiratory tract infection were associated in a few patients. Acute onset, short course and a high rate of intracranial bleeding (65%), particularly subdural and subarachnoid, were observed. The haemostatic defects appeared to be a marked reduction in factor II, VII, IX, and X. Complete blood counts remained relatively normal, with the exception of some changes (anaemia, leukocytosis), in response to the acute bleeding. Liver chemistry was normal or slightly impaired. No specific pathological changes were noted at autopsy, there were mild changes of liver cells, such as rare focal necrosis of liver cells, the proliferation of Kupffer cells, extramedullary haemopoeisis and mild cholestasis. Clinical improvement and correction of hemostatic defects were noted after vitamin K therapy alone or with fresh blood transfusion. Mortality rates were high in infants with intracranial bleeding (40-55%), while the overall mortality rate was 25%. The pathogenesis and the possibility of prevention of the syndrome were discussed.", "contents": "A bleeding syndrome in infants: acquired prothrombin complex deficiency of unknown aetiology. A total of 240 cases of a bleeding syndrome in infants due to prothrombin complex deficiency of unknown aetiology were reviewed. The majority of patients were breast fed, aged 1-2 months and the syndrome was more prevalent in males. Clinical manifestations consisted of bleeding, pallor and mild hepatomegaly in the majority of cases. Mild fever, diarrhoea, jaundice, and upper respiratory tract infection were associated in a few patients. Acute onset, short course and a high rate of intracranial bleeding (65%), particularly subdural and subarachnoid, were observed. The haemostatic defects appeared to be a marked reduction in factor II, VII, IX, and X. Complete blood counts remained relatively normal, with the exception of some changes (anaemia, leukocytosis), in response to the acute bleeding. Liver chemistry was normal or slightly impaired. No specific pathological changes were noted at autopsy, there were mild changes of liver cells, such as rare focal necrosis of liver cells, the proliferation of Kupffer cells, extramedullary haemopoeisis and mild cholestasis. Clinical improvement and correction of hemostatic defects were noted after vitamin K therapy alone or with fresh blood transfusion. Mortality rates were high in infants with intracranial bleeding (40-55%), while the overall mortality rate was 25%. The pathogenesis and the possibility of prevention of the syndrome were discussed."}
{"id": "PMID:1084025", "title": "Rupture of the left kidney following renosplenic shunt.", "content": "Kidney rupture following division of the left renal vein during portal decompression is presented. A review of the renal venous anatomy is described and a possible explanation of this ususual occurrence is offered.", "contents": "Rupture of the left kidney following renosplenic shunt. Kidney rupture following division of the left renal vein during portal decompression is presented. A review of the renal venous anatomy is described and a possible explanation of this ususual occurrence is offered."}
{"id": "PMID:1084026", "title": "The late results of terminal esophagoproximal gastrectomy (TEPG) with intensive devascularization and splenectomy for bleeding esophageal varices in cirrhosis.", "content": "The operative procedure of terminal esophagoproximal gastrectomy (TEPG) with extensive esophagogastric devascularization and splenectomy is described in detail and its rationale is discussed. Late results of this operation performed therapeutically on 64 patients with posthepatitic cirrhosis revealed that (1) operative mortality rate (death within a month) was 11%, mostly being contributed by poor-risk patients; (2) over-all survival was 65% at 5 years and 59% at 10 years; (3) gastrointestinal bleeding recurred in nine patients, five of whom had major and four minor hemorrhages; (4) portasystemic encephalopathy was seen frequently after shunts had never occurred, and the hepatic encephalopathy was only a sequel of major gastrointestinal bleeding or a manifestation of terminal-stage liver insufficiency; and (5) ascites was not a major clinical problem after this operation; mild esophagitis was an occasional complication.", "contents": "The late results of terminal esophagoproximal gastrectomy (TEPG) with intensive devascularization and splenectomy for bleeding esophageal varices in cirrhosis. The operative procedure of terminal esophagoproximal gastrectomy (TEPG) with extensive esophagogastric devascularization and splenectomy is described in detail and its rationale is discussed. Late results of this operation performed therapeutically on 64 patients with posthepatitic cirrhosis revealed that (1) operative mortality rate (death within a month) was 11%, mostly being contributed by poor-risk patients; (2) over-all survival was 65% at 5 years and 59% at 10 years; (3) gastrointestinal bleeding recurred in nine patients, five of whom had major and four minor hemorrhages; (4) portasystemic encephalopathy was seen frequently after shunts had never occurred, and the hepatic encephalopathy was only a sequel of major gastrointestinal bleeding or a manifestation of terminal-stage liver insufficiency; and (5) ascites was not a major clinical problem after this operation; mild esophagitis was an occasional complication."}
{"id": "PMID:1084027", "title": "[The tourniquet-syndrome--a severe complication after embolectomy of saddle embolism (author's transl)].", "content": "The symptoms of the tourniquet syndrome (hypotonia, tachycardia, postischemic edema, hypercaliemia, metabolic acidosis, myoglobinuria, renal insufficiency) could be observed after embolectomy of saddle embolism in 37 patients. 19 patients died postoperatively; heart insufficiency was demonstrated by autopsy in 14 patients. The pathophysiology of heart failure in tourniquet syndrome was studied in dog experiments after unilateral and bilateral hind limb ischemia. The development of shock turned out to be more severe after bilateral ischemia--comparable to saddle embolism--than after unilateral ischemia. The course of heart failure after recirculation could be referred to hypercaliemia, hypermagnesiemia, metabolic acidosis and hemoconcentration. The prophylaxis of the tourniquet syndrome can be practised by knowing the pathogenesis.", "contents": "[The tourniquet-syndrome--a severe complication after embolectomy of saddle embolism (author's transl)]. The symptoms of the tourniquet syndrome (hypotonia, tachycardia, postischemic edema, hypercaliemia, metabolic acidosis, myoglobinuria, renal insufficiency) could be observed after embolectomy of saddle embolism in 37 patients. 19 patients died postoperatively; heart insufficiency was demonstrated by autopsy in 14 patients. The pathophysiology of heart failure in tourniquet syndrome was studied in dog experiments after unilateral and bilateral hind limb ischemia. The development of shock turned out to be more severe after bilateral ischemia--comparable to saddle embolism--than after unilateral ischemia. The course of heart failure after recirculation could be referred to hypercaliemia, hypermagnesiemia, metabolic acidosis and hemoconcentration. The prophylaxis of the tourniquet syndrome can be practised by knowing the pathogenesis."}
{"id": "PMID:1084028", "title": "[Early postoperative diagnostic management by arterial-reobstruction after vascular operations using the ultrasonic flowmeter (author's transl)].", "content": "The Doppler ultrasonic method can confirm the success of the operation in the early post-operative phase after reconstructive vascular operations as an essential non-invasive technique or clear a doubtful improvement of the blood flow. Thus an arteriography can be indicated in good time. On the basis of reconstructive cases the usefulness and the advantageous application of the Doppler ultrasonic flowmeter in the operating theater is demonstrated.", "contents": "[Early postoperative diagnostic management by arterial-reobstruction after vascular operations using the ultrasonic flowmeter (author's transl)]. The Doppler ultrasonic method can confirm the success of the operation in the early post-operative phase after reconstructive vascular operations as an essential non-invasive technique or clear a doubtful improvement of the blood flow. Thus an arteriography can be indicated in good time. On the basis of reconstructive cases the usefulness and the advantageous application of the Doppler ultrasonic flowmeter in the operating theater is demonstrated."}
{"id": "PMID:1084029", "title": "[Paraplegia following acute infrarenal aortic occlusion (author's transl)].", "content": "The clinical syndrome of paraplegia following acute occlusion of the infrarenal aorta may be caused by either ischemic spinal cord damage or ischemia of the cauda equina and sacral nerve roots and ganglia. The neurologic manifestations are similar and therefore specific anatomic diagnosis is difficult. From October 1972 to February 1975 a total of 31 patients with infrarenal aortic occlusion were treated at the Medizinische Hochschule in Hannover. In nine cases the occlusion up to the renal arteries was acute. Three of these patients presented beside acute ischemic manifestations on both legs and the lower abdominal wall, neurologic symptoms of paraplegia. The anatomic and hemodynamic aspects of ischemic spinal cord damage and those of ischemic lesions of the cauda equina and peripheral nerves are discussed. There appear to be three main mechanisms responsible for vascular paraplegia following acute infrarenal occlusion of the aorta: 1. it may be caused by thrombotic occlusion of a major radicular artery which arises below the level of occlusion. 2. it may be produced by thrombosis of a lumbar collateral acting as major supply to the cord when arteriosclerotic narrowing of the major radicular artery is present. Especially in states of severe hypotension critical interference of blood supply to the spinal cord will result. 3. Paraplegia by ischemia of the cauda and peripheral nervous tissue may also follow prolonged interruption of circulation to this area supplying spinal vessels from low lumbar and sacral arteries.", "contents": "[Paraplegia following acute infrarenal aortic occlusion (author's transl)]. The clinical syndrome of paraplegia following acute occlusion of the infrarenal aorta may be caused by either ischemic spinal cord damage or ischemia of the cauda equina and sacral nerve roots and ganglia. The neurologic manifestations are similar and therefore specific anatomic diagnosis is difficult. From October 1972 to February 1975 a total of 31 patients with infrarenal aortic occlusion were treated at the Medizinische Hochschule in Hannover. In nine cases the occlusion up to the renal arteries was acute. Three of these patients presented beside acute ischemic manifestations on both legs and the lower abdominal wall, neurologic symptoms of paraplegia. The anatomic and hemodynamic aspects of ischemic spinal cord damage and those of ischemic lesions of the cauda equina and peripheral nerves are discussed. There appear to be three main mechanisms responsible for vascular paraplegia following acute infrarenal occlusion of the aorta: 1. it may be caused by thrombotic occlusion of a major radicular artery which arises below the level of occlusion. 2. it may be produced by thrombosis of a lumbar collateral acting as major supply to the cord when arteriosclerotic narrowing of the major radicular artery is present. Especially in states of severe hypotension critical interference of blood supply to the spinal cord will result. 3. Paraplegia by ischemia of the cauda and peripheral nervous tissue may also follow prolonged interruption of circulation to this area supplying spinal vessels from low lumbar and sacral arteries."}
{"id": "PMID:1084030", "title": "[On-line monitoring of cardiac output with a new pulse contour computer (author's transl)].", "content": "A new pulse contour cardiac output computer, operating from the aortic pressure wave form was tested in cardiac surgical patients. Reference cardiac output was measured by thermal dilution technique. Satisfactory agreements were obtained between the thermal dilution and the COC-pulse-contour cardiac outputs when rapid variations of cardiac output occurred as well as slowly developing changes. The results show that the computation of the stroke volume from the aortic pressure wave form offers a simple, clinically useful method for on-line monitoring of cardiac output in critically ill patients. Possible error sources are discussed.", "contents": "[On-line monitoring of cardiac output with a new pulse contour computer (author's transl)]. A new pulse contour cardiac output computer, operating from the aortic pressure wave form was tested in cardiac surgical patients. Reference cardiac output was measured by thermal dilution technique. Satisfactory agreements were obtained between the thermal dilution and the COC-pulse-contour cardiac outputs when rapid variations of cardiac output occurred as well as slowly developing changes. The results show that the computation of the stroke volume from the aortic pressure wave form offers a simple, clinically useful method for on-line monitoring of cardiac output in critically ill patients. Possible error sources are discussed."}
{"id": "PMID:1084031", "title": "[Diagnosis, frequency and importance of the \"low-output-syndrome\" in the postoperative period after mitral valve replacement (author's transl)].", "content": "From 1970 until 1975 single mitral valve replacement was carried out in 162 patients using Bj\u00f6rk-Shiley disc prostheses. The surgical mortality (within 30 days postoperatively) was 9.3% (n=15). Out of these 15 patients 9 died due to myogenic cardiac insufficiency. 21 patients with \"Low-output-Syndrome\" due to myogenic insufficiency however survived. The improvement of intraoperative myocardial protection since 1972 (using hypothermia and cardioplegic induced cardiac arrest) reduced the frequency of myogenic cardiac insufficiency to 1%. Unrelated to the above mentioned we saw in 18.5% of all patients after ECC-procedures a temporary \"Low-output-Syndrome\" after the 3rd postoperative day due to pericardial effusion.", "contents": "[Diagnosis, frequency and importance of the \"low-output-syndrome\" in the postoperative period after mitral valve replacement (author's transl)]. From 1970 until 1975 single mitral valve replacement was carried out in 162 patients using Bj\u00f6rk-Shiley disc prostheses. The surgical mortality (within 30 days postoperatively) was 9.3% (n=15). Out of these 15 patients 9 died due to myogenic cardiac insufficiency. 21 patients with \"Low-output-Syndrome\" due to myogenic insufficiency however survived. The improvement of intraoperative myocardial protection since 1972 (using hypothermia and cardioplegic induced cardiac arrest) reduced the frequency of myogenic cardiac insufficiency to 1%. Unrelated to the above mentioned we saw in 18.5% of all patients after ECC-procedures a temporary \"Low-output-Syndrome\" after the 3rd postoperative day due to pericardial effusion."}
{"id": "PMID:1084032", "title": "[Acute left heart failure following repair of atrial septal defects. Its treatment by reopening].", "content": "Among 716 patients operated on for an ASD, 15 of them, following closure of the defect, developed acute left heart failure requiring partial re-opening. The most prominent anatomical finding in these cases was hypoplasia of the left ventricle. Also remarkable in some patients was severe pulmonary hypertension, which may have led to right ventricular hypertrophy. Therefore, a marked difference of the stroke work of the left and right ventricle, respectively, appeared to be the essential underlying hemodynamic mechanism. The prognosis has been found to depend mainly on prompt surgical intervention; so in all poor-risk cases continued monitoring of left atrial pressure is indicated for early detection of impending left heart failure.--The high incidence of left heart failure as cause of death after repair of an ASD indicates the importance of this complication.", "contents": "[Acute left heart failure following repair of atrial septal defects. Its treatment by reopening]. Among 716 patients operated on for an ASD, 15 of them, following closure of the defect, developed acute left heart failure requiring partial re-opening. The most prominent anatomical finding in these cases was hypoplasia of the left ventricle. Also remarkable in some patients was severe pulmonary hypertension, which may have led to right ventricular hypertrophy. Therefore, a marked difference of the stroke work of the left and right ventricle, respectively, appeared to be the essential underlying hemodynamic mechanism. The prognosis has been found to depend mainly on prompt surgical intervention; so in all poor-risk cases continued monitoring of left atrial pressure is indicated for early detection of impending left heart failure.--The high incidence of left heart failure as cause of death after repair of an ASD indicates the importance of this complication."}
{"id": "PMID:1084033", "title": "[Determination of serum-digitalisglycoside concentrations by radioimmunoassay before, during and after operations with extracorporal circulation (author's transl)].", "content": "In 13 adult patients serum-glycoside concentrations, renal glycoside elimination and endogenic creatinine clearance were determined before, during and in the first 4 days following the operation. A postoperative digitalis cumulation has not been seen. But there was a diminished digitalis tolerance in the immediate postoperative phase. Two patients got ventricular bigeminus. The low potassium values at the same time may possibly affect the sensitivity of digitalis.", "contents": "[Determination of serum-digitalisglycoside concentrations by radioimmunoassay before, during and after operations with extracorporal circulation (author's transl)]. In 13 adult patients serum-glycoside concentrations, renal glycoside elimination and endogenic creatinine clearance were determined before, during and in the first 4 days following the operation. A postoperative digitalis cumulation has not been seen. But there was a diminished digitalis tolerance in the immediate postoperative phase. Two patients got ventricular bigeminus. The low potassium values at the same time may possibly affect the sensitivity of digitalis."}
{"id": "PMID:1084034", "title": "[Effect of intraaortic-ballon-counterpulsation on the changes of myocardial blood flow following coronary artery stenosis and occlusion (author's transl)].", "content": "To prove the effectiveness of the IABP in case of impending myocardial infarction in one series of experiments myocardial blood flow changes due to IABP in dogs with stenosis of the left circumflex coronary artery (LC) were investigated using tracer microspheres. In a control series the left anterior descending artery (LAD) was occluded. In acute LAD occlusion the IABP caused an increase of blood flow in the epicardial layer. In case of LC stenosis there was a far greater increase in blood flow by the IABP most prominent in the endocardial layer. Our experiments indicate that the IABP seems to be effective in impending myocardial infarction.", "contents": "[Effect of intraaortic-ballon-counterpulsation on the changes of myocardial blood flow following coronary artery stenosis and occlusion (author's transl)]. To prove the effectiveness of the IABP in case of impending myocardial infarction in one series of experiments myocardial blood flow changes due to IABP in dogs with stenosis of the left circumflex coronary artery (LC) were investigated using tracer microspheres. In a control series the left anterior descending artery (LAD) was occluded. In acute LAD occlusion the IABP caused an increase of blood flow in the epicardial layer. In case of LC stenosis there was a far greater increase in blood flow by the IABP most prominent in the endocardial layer. Our experiments indicate that the IABP seems to be effective in impending myocardial infarction."}
{"id": "PMID:1084035", "title": "[Improvement of myocardial blood flow and cardiac hemodynamic by means of intraaortic balloon pumping IABP during experimental heart failure (author's transl)].", "content": "The influence of the intraaortic balloon pumping (IABP) on the hemodynamic and coronary blood flow under control conditions and after experimental heart failure induced by coronary artery ligature was proved on eight dogs. By timely use of IABP (systolic pressure over 60-70 mmHg), the cardiac hemodynamics can be improved by means of reducing myocardial oxygen demands, and on the other hand by raising the coronary perfusion and oxygen supply.", "contents": "[Improvement of myocardial blood flow and cardiac hemodynamic by means of intraaortic balloon pumping IABP during experimental heart failure (author's transl)]. The influence of the intraaortic balloon pumping (IABP) on the hemodynamic and coronary blood flow under control conditions and after experimental heart failure induced by coronary artery ligature was proved on eight dogs. By timely use of IABP (systolic pressure over 60-70 mmHg), the cardiac hemodynamics can be improved by means of reducing myocardial oxygen demands, and on the other hand by raising the coronary perfusion and oxygen supply."}
{"id": "PMID:1084036", "title": "[Intra-aortic balloon pumping in cardiogenic shock after cardiac surgery (author's transl)].", "content": "The results of intra-aortic balloon pumping in 7 patients with cardiogenic shock after cardiac surgery are reported. 2 patients were unresponsive to IABP, 4 patients showed initial stabilization of the circulation, but became IABP-dependent and died. One patient could be weaned from IABP, but died of uncontrollable cardiac arrhythmias. These results are discussed.", "contents": "[Intra-aortic balloon pumping in cardiogenic shock after cardiac surgery (author's transl)]. The results of intra-aortic balloon pumping in 7 patients with cardiogenic shock after cardiac surgery are reported. 2 patients were unresponsive to IABP, 4 patients showed initial stabilization of the circulation, but became IABP-dependent and died. One patient could be weaned from IABP, but died of uncontrollable cardiac arrhythmias. These results are discussed."}
{"id": "PMID:1084037", "title": "[Diagnostic and therapy of postoperative cardiac low-output-syndrome by means of intraaortic balloon pumping (IABP) or external counterpulsation (ECP)--a comparison (author's transl)].", "content": "A beneficial influence on the myocardial energy imbalance of the hypoxaemically damaged heart can be reached with either intraaortic balloon pumping (IABP) or external counterpulsation (ECP). In the realm of our acquired clinical experience with two groups of patients, the technical demands of these cardiac assist devices are discussed and distinguished from one another in relation to different indications.", "contents": "[Diagnostic and therapy of postoperative cardiac low-output-syndrome by means of intraaortic balloon pumping (IABP) or external counterpulsation (ECP)--a comparison (author's transl)]. A beneficial influence on the myocardial energy imbalance of the hypoxaemically damaged heart can be reached with either intraaortic balloon pumping (IABP) or external counterpulsation (ECP). In the realm of our acquired clinical experience with two groups of patients, the technical demands of these cardiac assist devices are discussed and distinguished from one another in relation to different indications."}
{"id": "PMID:1084038", "title": "[Respiratory care in infants following cardiac surgery (author's transl)].", "content": "Report on results in postoperative intensive care of 73 infants. 37 underwent corrective surgery on extracorporeal circulation (mortality 24,3%), 36 were operated on without ECC (mortality 16.7%). All infants had been intubated by the nasotracheal route, 39 were treated with CPPV (mean duration 32.5 h), 23 with IPPV (mean duration 28 h). Almost 50% were treated with +7 cm H2O CPAP (mean duration 32 h). Advantages and potential hazards of CPPV and CPAP are discussed.", "contents": "[Respiratory care in infants following cardiac surgery (author's transl)]. Report on results in postoperative intensive care of 73 infants. 37 underwent corrective surgery on extracorporeal circulation (mortality 24,3%), 36 were operated on without ECC (mortality 16.7%). All infants had been intubated by the nasotracheal route, 39 were treated with CPPV (mean duration 32.5 h), 23 with IPPV (mean duration 28 h). Almost 50% were treated with +7 cm H2O CPAP (mean duration 32 h). Advantages and potential hazards of CPPV and CPAP are discussed."}
{"id": "PMID:1084039", "title": "[Disturbances of ventilatory distribution as a cause of postoperative hypoxemia after cardio-thoracic surgery (author's transl)].", "content": "In a group of 21 patients with thoracotomy the lung function was investigated pre-and postoperatively with particular consideration to N2-clearance. From the N2-clearance an Inspiratory Gas Distribution Index (IDI) is derived. This Index allows in combination with blood gas analysis an interpretation of ventilatory disturbances in the lung.", "contents": "[Disturbances of ventilatory distribution as a cause of postoperative hypoxemia after cardio-thoracic surgery (author's transl)]. In a group of 21 patients with thoracotomy the lung function was investigated pre-and postoperatively with particular consideration to N2-clearance. From the N2-clearance an Inspiratory Gas Distribution Index (IDI) is derived. This Index allows in combination with blood gas analysis an interpretation of ventilatory disturbances in the lung."}
{"id": "PMID:1084040", "title": "[Postoperative analgetics in patients after thorax surgery (author's transl)].", "content": "The amount and sort of analgetics given postoperatively in 1000 patients who had major heart or lung surgery was statistically analysed. A critical review of comparable literature revealed that all about analgetics usually is estimated. New and more objective criteria were found.", "contents": "[Postoperative analgetics in patients after thorax surgery (author's transl)]. The amount and sort of analgetics given postoperatively in 1000 patients who had major heart or lung surgery was statistically analysed. A critical review of comparable literature revealed that all about analgetics usually is estimated. New and more objective criteria were found."}
{"id": "PMID:1084041", "title": "[Postoperatives acute renal failure after operations with extracorporeal circulation (author's transl)].", "content": "An acute renal failure was observed postoperatively in 3,45% of 929 patients operated with the heart lung machine during the years 1970-1972. Because of the bad results of any therapy in the case of acute postoperative renal failure, the diagnostic procedures of the preoperative renal function were intensified of the preoperative renal function were intensified in order to prevent an acute postoperative renal failure. Therefore additional measurements of the creatinine clearance and in few cases radioisotopic clearance tests were done.", "contents": "[Postoperatives acute renal failure after operations with extracorporeal circulation (author's transl)]. An acute renal failure was observed postoperatively in 3,45% of 929 patients operated with the heart lung machine during the years 1970-1972. Because of the bad results of any therapy in the case of acute postoperative renal failure, the diagnostic procedures of the preoperative renal function were intensified of the preoperative renal function were intensified in order to prevent an acute postoperative renal failure. Therefore additional measurements of the creatinine clearance and in few cases radioisotopic clearance tests were done."}
{"id": "PMID:1084042", "title": "[Drug induced acute renal failure after heart surgery (author's transl)].", "content": "The incidence of acute renal failure following cardiac surgery was markedly increased during the last years in our medical center. There was no correlation to known etiologic factors such as preexisting disease, hemolysis, low perfusion rates, post-operative low output syndrome etc. Post-operative renal failure was mainly seen in children between 2 and 10 years and was noted after 3-4 days of normal renal function. Peritoneal dialysis was carried out in 39% of out patients and the overall mortality was 10.7%. Careful analysis of our cases exhibited a correlation of the post operative acute renal failure to the intravenous application of Aescin. After abandoning this drug from our post-operative therapy we did not observe a similar case since almost one year.", "contents": "[Drug induced acute renal failure after heart surgery (author's transl)]. The incidence of acute renal failure following cardiac surgery was markedly increased during the last years in our medical center. There was no correlation to known etiologic factors such as preexisting disease, hemolysis, low perfusion rates, post-operative low output syndrome etc. Post-operative renal failure was mainly seen in children between 2 and 10 years and was noted after 3-4 days of normal renal function. Peritoneal dialysis was carried out in 39% of out patients and the overall mortality was 10.7%. Careful analysis of our cases exhibited a correlation of the post operative acute renal failure to the intravenous application of Aescin. After abandoning this drug from our post-operative therapy we did not observe a similar case since almost one year."}
{"id": "PMID:1084043", "title": "[Treatment of postoperative renal insufficiency after cardiovascular surgical procedures by peritoneal dialysis (author's transl)].", "content": "In 1972 and 1973 there have been 698 (351 with ECC) cardiovascular surgical procedures. 29 cases of renal insufficiency were seen in this group, 15 requiring peritoneal dialysis. There were only two survivors in the latter group. However none died due to renal insufficiency. The indication, technique, complications, results are discussed in detail.", "contents": "[Treatment of postoperative renal insufficiency after cardiovascular surgical procedures by peritoneal dialysis (author's transl)]. In 1972 and 1973 there have been 698 (351 with ECC) cardiovascular surgical procedures. 29 cases of renal insufficiency were seen in this group, 15 requiring peritoneal dialysis. There were only two survivors in the latter group. However none died due to renal insufficiency. The indication, technique, complications, results are discussed in detail."}
{"id": "PMID:1084044", "title": "[Experiences in the treatment of acute renal failure following cardiovascular surgery (author's transl)].", "content": "After 9021 cardiovascular operations 210 patients developed acute renal failure. 140 (67%) out of this group died. The mortality rate of acute renal failure could be reduced from 80% (1958-1966) to 50% (1973-1974). Dialysis treatment should begin early and also in special cases, in which the cause of acute renal failure is not under control. Beyond optimal dialysis therapy common intensive care is important for the prognosis of acute renal failure, especially adaption of drugs to renal function (antibiotics and digitalis), high-caloric alimentation, consequent treatment of infections, strict balance of fluid and electrolytes.", "contents": "[Experiences in the treatment of acute renal failure following cardiovascular surgery (author's transl)]. After 9021 cardiovascular operations 210 patients developed acute renal failure. 140 (67%) out of this group died. The mortality rate of acute renal failure could be reduced from 80% (1958-1966) to 50% (1973-1974). Dialysis treatment should begin early and also in special cases, in which the cause of acute renal failure is not under control. Beyond optimal dialysis therapy common intensive care is important for the prognosis of acute renal failure, especially adaption of drugs to renal function (antibiotics and digitalis), high-caloric alimentation, consequent treatment of infections, strict balance of fluid and electrolytes."}
{"id": "PMID:1084045", "title": "[Mortality and preoperative left ventricular function in aortocoronary bypass surgery (author's transl)].", "content": "Left ventricular function in 8 patients with coronary heart disease, who died during or immediately after surgery, was compared with the corresponding data of 25 survivors. Both groups were similar in age and had about the same number of myocardial infarctions preoperatively. For LVEDP, EDV, EF and VCF critical values could be defined, which were found in none of the survivors. These values were: LVDEP approximately 20 mm Hg, EDV approximately 150 ml/m2, EF less than 30% and VCF less than 0,4 circ/s. The least prognostic significance was found for LVEDP; EDV and VCF correlated best with the surgical risk.", "contents": "[Mortality and preoperative left ventricular function in aortocoronary bypass surgery (author's transl)]. Left ventricular function in 8 patients with coronary heart disease, who died during or immediately after surgery, was compared with the corresponding data of 25 survivors. Both groups were similar in age and had about the same number of myocardial infarctions preoperatively. For LVEDP, EDV, EF and VCF critical values could be defined, which were found in none of the survivors. These values were: LVDEP approximately 20 mm Hg, EDV approximately 150 ml/m2, EF less than 30% and VCF less than 0,4 circ/s. The least prognostic significance was found for LVEDP; EDV and VCF correlated best with the surgical risk."}
{"id": "PMID:1084046", "title": "[Acupuncture analgesia and controlled respiration. A new modified method of anesthesia in open heart surgery (author's transl)].", "content": "From October 1973 to February 1975 cardiac surgery with extracorporal circulation using Acupuncture as analgesia was performed in 88 patients in the surgical clinic of the University of Giessen. Acupuncture is performed by electric-stimulation of 8 points at the lower legs, the neck and ears with 90 V and 5-6 Hz. All patients are intubated; controlled respiration is maintained with 50% O2 and 50% N2O. During the whole operation, 20 minutes after intubation the patients are responsible. The main advantage of the new method we see in a good postoperative cooperation of the patients, which are generally extubabed in the operation room.", "contents": "[Acupuncture analgesia and controlled respiration. A new modified method of anesthesia in open heart surgery (author's transl)]. From October 1973 to February 1975 cardiac surgery with extracorporal circulation using Acupuncture as analgesia was performed in 88 patients in the surgical clinic of the University of Giessen. Acupuncture is performed by electric-stimulation of 8 points at the lower legs, the neck and ears with 90 V and 5-6 Hz. All patients are intubated; controlled respiration is maintained with 50% O2 and 50% N2O. During the whole operation, 20 minutes after intubation the patients are responsible. The main advantage of the new method we see in a good postoperative cooperation of the patients, which are generally extubabed in the operation room."}
{"id": "PMID:1084047", "title": "[Immunglobulin levels and alterations of the lymphocyte function after extracorporal circulation and massive blood transfusion (author's transl)].", "content": "Cardiovascular operations are connected with an increased risk of infection. Postoperatively there happened a temporary fall of all immunglobulin levels. Concerning the cellular immunity, we found an impressive decrease of the transformation of the lymphocytes in the presence of polyclonal mitogens which normalises only partially after the eighth day. These results refer to a complex alteration of the immunological apparatus, imposing as a temporary immundeficiency, and could contribute to an elucidation of the increased risk of infections.", "contents": "[Immunglobulin levels and alterations of the lymphocyte function after extracorporal circulation and massive blood transfusion (author's transl)]. Cardiovascular operations are connected with an increased risk of infection. Postoperatively there happened a temporary fall of all immunglobulin levels. Concerning the cellular immunity, we found an impressive decrease of the transformation of the lymphocytes in the presence of polyclonal mitogens which normalises only partially after the eighth day. These results refer to a complex alteration of the immunological apparatus, imposing as a temporary immundeficiency, and could contribute to an elucidation of the increased risk of infections."}
{"id": "PMID:1084048", "title": "[The influence of increased clotting reactions as shown by thrombosis formation in the immediate postoperative period on aortic valve prothesis (author's transl)].", "content": "Massive thrombosis formation on the valve periphery and on the top surface of the valve occurred in three patients (1,5%) in the immediate postoperative period with the Bj\u00f6rk-Shiley valve in the aortic position. Between the third and sixth day these patients died of acute heart failure as a result of coronary artery displacement. Upon autopsy operative technical complications and postoperative infections were ruled out as the cause of death. What appears to be clinically important is an increase in clotting time in the immediate postoperative period which can be proven statistically. This increased clotting inclination was only found in these three patients and in one patient with frequent immediate postoperative peripheral embolic episodes. We therefore feel that early anticoagulation therapy is necessary. Heparin administration is preferred as it not only lowers the clotting ability of the blood but also the adhesive quality of the platelets.", "contents": "[The influence of increased clotting reactions as shown by thrombosis formation in the immediate postoperative period on aortic valve prothesis (author's transl)]. Massive thrombosis formation on the valve periphery and on the top surface of the valve occurred in three patients (1,5%) in the immediate postoperative period with the Bj\u00f6rk-Shiley valve in the aortic position. Between the third and sixth day these patients died of acute heart failure as a result of coronary artery displacement. Upon autopsy operative technical complications and postoperative infections were ruled out as the cause of death. What appears to be clinically important is an increase in clotting time in the immediate postoperative period which can be proven statistically. This increased clotting inclination was only found in these three patients and in one patient with frequent immediate postoperative peripheral embolic episodes. We therefore feel that early anticoagulation therapy is necessary. Heparin administration is preferred as it not only lowers the clotting ability of the blood but also the adhesive quality of the platelets."}
{"id": "PMID:1084049", "title": "[Heart valve surgery in bacterial endocarditis (author's transl)].", "content": "Bacterial endocarditis was the cause of heart valve destruction in 23 patients. Additional 5 patients were reoperated because of an infected valve prothesis. 13 were operated upon during the inactive stage. Nevertheless infection recurred after operation at least in one patient. 10 patients underwent operation during the active stage, 6 of these with stable cardiac function, 4 in cardiogenic shock. Hospital mortality was 2/6 and 1/4 respectively. One patient died after three months of persisting infection. Three of five patients with infected valve prosthesis survived reoperation. An attempt is made to define the indication for valve surgery in bacterial endocarditis.", "contents": "[Heart valve surgery in bacterial endocarditis (author's transl)]. Bacterial endocarditis was the cause of heart valve destruction in 23 patients. Additional 5 patients were reoperated because of an infected valve prothesis. 13 were operated upon during the inactive stage. Nevertheless infection recurred after operation at least in one patient. 10 patients underwent operation during the active stage, 6 of these with stable cardiac function, 4 in cardiogenic shock. Hospital mortality was 2/6 and 1/4 respectively. One patient died after three months of persisting infection. Three of five patients with infected valve prosthesis survived reoperation. An attempt is made to define the indication for valve surgery in bacterial endocarditis."}
{"id": "PMID:1084050", "title": "[Reversibility or irreversibility of pulmonary function changes after cardiac surgery on insufficient or stenotic cardiac valves (author's transl)].", "content": "In 69 patients with mitral- or aortic valves disease lung function tests were made preoperatively and 2 to 4 years after cardiac surgery. We wanted to demonstrate the extent and the improvement of restriction, obstruction and disturbed diffusing capacity of the separate diseases with different severities. We found correlations between the clinical grade of the disease and the changes in lung function. In particular grade III of the mitral stenosis showed severe restriction, obstruction and disturbed diffusing capacity. Patients with mitral insufficiency III had changes of mild degree, aortic diseases were without disturbed lung function. All detected changes of lung function were irreversible and could not be influenced by postoperative hemodynamic improvements.", "contents": "[Reversibility or irreversibility of pulmonary function changes after cardiac surgery on insufficient or stenotic cardiac valves (author's transl)]. In 69 patients with mitral- or aortic valves disease lung function tests were made preoperatively and 2 to 4 years after cardiac surgery. We wanted to demonstrate the extent and the improvement of restriction, obstruction and disturbed diffusing capacity of the separate diseases with different severities. We found correlations between the clinical grade of the disease and the changes in lung function. In particular grade III of the mitral stenosis showed severe restriction, obstruction and disturbed diffusing capacity. Patients with mitral insufficiency III had changes of mild degree, aortic diseases were without disturbed lung function. All detected changes of lung function were irreversible and could not be influenced by postoperative hemodynamic improvements."}
{"id": "PMID:1084051", "title": "[The correlation between pulmonary hypertension and histological findings of the pulmonary vessels in congenital heart disease (author's transl)].", "content": "In nearly 100 infants the h\u00e4modynamic and histological findings of pulmonary hypertension in congenital heart diseases are correlated when a banding-procedure was done. There were: 45 ventricle septal defects, 10 atrio-ventricular canals, 13 transpositions of the great arteries, 16 patent ductus arteriosus and 7 miscellaneous. A h\u00e4modynamic hypertension was more frequent than the histological hypertension; but there was a difference in the different heart diseases: The patients with patent ductus arteriosus and hypertension show only in a few cases histological alterations; in infants with VSD the correlation was much more better. Whether a banding procedure of the pulmonary artery is able to provide the progress of pulmonary sclerosis may be discussed after a second excision of lung-tissue when the correction of the heart disease is done.", "contents": "[The correlation between pulmonary hypertension and histological findings of the pulmonary vessels in congenital heart disease (author's transl)]. In nearly 100 infants the h\u00e4modynamic and histological findings of pulmonary hypertension in congenital heart diseases are correlated when a banding-procedure was done. There were: 45 ventricle septal defects, 10 atrio-ventricular canals, 13 transpositions of the great arteries, 16 patent ductus arteriosus and 7 miscellaneous. A h\u00e4modynamic hypertension was more frequent than the histological hypertension; but there was a difference in the different heart diseases: The patients with patent ductus arteriosus and hypertension show only in a few cases histological alterations; in infants with VSD the correlation was much more better. Whether a banding procedure of the pulmonary artery is able to provide the progress of pulmonary sclerosis may be discussed after a second excision of lung-tissue when the correction of the heart disease is done."}
{"id": "PMID:1084052", "title": "[Atypical aortic coarctation (author's transl)].", "content": "Special problems in the surgical treatment of aortic coarctation may be caused by unusual morphological findings as a long hypoplastic segment, the combination with pre- or poststenotic aneurysms or by atypical localisation of the stenosis in the aortic arch, the decreasing or abdominal aorta. A bypass procedure is considered as the method of choice for the repair of coarctation in the aortic arch or the \"critical zone\" of the abdominal aorta (renal- and suprarenal segment). The bypass-principle allows many variations of branching and avoids cross-clamping of the aorta. The involvement of important branches as is the left common carotid, the superior mesenteric or the renal artery, necessitates a vascular reconstruction in the same session. Probably the first report about a very rare combined lesions is presented: the combination of an aortic arch coarctation in association with a complete situs inversus (right descending aorta), a common carotid trunc, an aneurysm of the left subclavian artery (first branch of the arch) and congenital cysts of the right upper lobe of the lung (Case No. 1). Usually concomitant aneurysms are found in the pre- or poststenotic aortic segment. Some of the infrarenal fusiform aneurysms (Case No. 4 and 5) are occasionally of poststenotic origin, secondary to a longstanding infrarenal coarctation. In these cases the procedure of choice is the total exstirpation and the interposition of a Dacrongraft. Important branches (as the subclavian or renal artery) can be some times inserted directly in the vascular protheses. Using the bypass technic or the patch graftplastic of Vosschulte the risk of late operation (beyond the age of 30 years) may be remarkably reduced.", "contents": "[Atypical aortic coarctation (author's transl)]. Special problems in the surgical treatment of aortic coarctation may be caused by unusual morphological findings as a long hypoplastic segment, the combination with pre- or poststenotic aneurysms or by atypical localisation of the stenosis in the aortic arch, the decreasing or abdominal aorta. A bypass procedure is considered as the method of choice for the repair of coarctation in the aortic arch or the \"critical zone\" of the abdominal aorta (renal- and suprarenal segment). The bypass-principle allows many variations of branching and avoids cross-clamping of the aorta. The involvement of important branches as is the left common carotid, the superior mesenteric or the renal artery, necessitates a vascular reconstruction in the same session. Probably the first report about a very rare combined lesions is presented: the combination of an aortic arch coarctation in association with a complete situs inversus (right descending aorta), a common carotid trunc, an aneurysm of the left subclavian artery (first branch of the arch) and congenital cysts of the right upper lobe of the lung (Case No. 1). Usually concomitant aneurysms are found in the pre- or poststenotic aortic segment. Some of the infrarenal fusiform aneurysms (Case No. 4 and 5) are occasionally of poststenotic origin, secondary to a longstanding infrarenal coarctation. In these cases the procedure of choice is the total exstirpation and the interposition of a Dacrongraft. Important branches (as the subclavian or renal artery) can be some times inserted directly in the vascular protheses. Using the bypass technic or the patch graftplastic of Vosschulte the risk of late operation (beyond the age of 30 years) may be remarkably reduced."}
{"id": "PMID:1084053", "title": "[Congenital incomplete aortic-arch-syndrome as a rare cause of cerebral ischemia in consequence of a steal-syndrome (author's transl)].", "content": "A subclavian-steal-syndrome of a congenital origin is a rare observation. The authors report on a 27 years old female patient with characteristic symptoms. The vascular deformity consisted of a right-sided aortic arch, a right descending aorta, separate origins of the anonymous and the right carotid arteries, and an aplasia of the proximal parts of the left carotid and the left subclavian arteries. A combined dacron-venous-bypass was placed from the ascending aorta to the left arm-neck arteries and thus cerebral circulation was improved and the patient got free from symptoms. Special problems of morphology and hemodynamics are discussed on the hand of arteriograms.", "contents": "[Congenital incomplete aortic-arch-syndrome as a rare cause of cerebral ischemia in consequence of a steal-syndrome (author's transl)]. A subclavian-steal-syndrome of a congenital origin is a rare observation. The authors report on a 27 years old female patient with characteristic symptoms. The vascular deformity consisted of a right-sided aortic arch, a right descending aorta, separate origins of the anonymous and the right carotid arteries, and an aplasia of the proximal parts of the left carotid and the left subclavian arteries. A combined dacron-venous-bypass was placed from the ascending aorta to the left arm-neck arteries and thus cerebral circulation was improved and the patient got free from symptoms. Special problems of morphology and hemodynamics are discussed on the hand of arteriograms."}
{"id": "PMID:1084054", "title": "[Rupture of the right atrium following blunt trauma of the thorax (author's transl)].", "content": "A rupture of the right atrium following blunt trauma of the thorax was operated with success. The effectiveness of different diagnostic procedures is discussed.", "contents": "[Rupture of the right atrium following blunt trauma of the thorax (author's transl)]. A rupture of the right atrium following blunt trauma of the thorax was operated with success. The effectiveness of different diagnostic procedures is discussed."}
{"id": "PMID:1084055", "title": "[Single staged correction of congenital heart disease with patent ductus arteriosus: ductclosure with metal clip via a median sternotomy incision (author's transl)].", "content": "Using the Hemoclips a patent ductus arteriosus can easily be closed at its pulmonary origin via a median sternotomy incision. This enlarges the surgical indication for single staged correction of congenital heart lesions combined with a patent ductus arteriosus.", "contents": "[Single staged correction of congenital heart disease with patent ductus arteriosus: ductclosure with metal clip via a median sternotomy incision (author's transl)]. Using the Hemoclips a patent ductus arteriosus can easily be closed at its pulmonary origin via a median sternotomy incision. This enlarges the surgical indication for single staged correction of congenital heart lesions combined with a patent ductus arteriosus."}
{"id": "PMID:1084056", "title": "[Different ultrastructure of the right and left myocardium after preservation for 24 hours (author's transl)].", "content": "After a 24 hrs' preservation of rat hearts, in 22% the viability test with allogenic perfusion showed a singular recovery of the right ventricle (57 of 257 experiments). The ultrastructural aspects correlate with the differences of the function. The micrographs of the right myocardium show--except a mild edema--ultrastructural integrity, but the micrographs of the left myocardium show marked alterations of the ultrastructure: edema, swollen mitochondria with destroyed cristal membranes, swelling of the sacrotubular system and alterations of myofibrils.", "contents": "[Different ultrastructure of the right and left myocardium after preservation for 24 hours (author's transl)]. After a 24 hrs' preservation of rat hearts, in 22% the viability test with allogenic perfusion showed a singular recovery of the right ventricle (57 of 257 experiments). The ultrastructural aspects correlate with the differences of the function. The micrographs of the right myocardium show--except a mild edema--ultrastructural integrity, but the micrographs of the left myocardium show marked alterations of the ultrastructure: edema, swollen mitochondria with destroyed cristal membranes, swelling of the sacrotubular system and alterations of myofibrils."}
{"id": "PMID:1084058", "title": "[Mediastinal chondrosarcoma (author's transl)].", "content": "A 41 years old female patient was operated on account of mediastinal tumour. A chondrosarcoma of 10 X 8 X 8 cm was removed. The patient remained symptomfree and had no complaints over 4 years after the operation, but after this relapse and pulmonary metastasis developed. In connection with the demonstrated case the referring literature of mediastinal chondrosarcomas was surveyed and it could be established that this is the twentieth published case about mediastinal chondrosarcoma.", "contents": "[Mediastinal chondrosarcoma (author's transl)]. A 41 years old female patient was operated on account of mediastinal tumour. A chondrosarcoma of 10 X 8 X 8 cm was removed. The patient remained symptomfree and had no complaints over 4 years after the operation, but after this relapse and pulmonary metastasis developed. In connection with the demonstrated case the referring literature of mediastinal chondrosarcomas was surveyed and it could be established that this is the twentieth published case about mediastinal chondrosarcoma."}
{"id": "PMID:1084059", "title": "[Pulmonary sequestration combined with Bochdalek's hernia (author's transl)].", "content": "Representation on a case of lung sequestration in combination with a hernia of Bochdalek in a nine weeks old infant. A high standing right diaphragm, diagnosed through the X-ray pictures, commanded thoracotomy as choice approach to the diaphragm. On the inspection, we found a Bochdalek hernia and as an incidental finding, on the same side, a partly extralobar, partly intralobar sequestration of the lung.", "contents": "[Pulmonary sequestration combined with Bochdalek's hernia (author's transl)]. Representation on a case of lung sequestration in combination with a hernia of Bochdalek in a nine weeks old infant. A high standing right diaphragm, diagnosed through the X-ray pictures, commanded thoracotomy as choice approach to the diaphragm. On the inspection, we found a Bochdalek hernia and as an incidental finding, on the same side, a partly extralobar, partly intralobar sequestration of the lung."}
{"id": "PMID:1084060", "title": "[Pulmonary rhabdomyosarcoma (author's transl)].", "content": "A 55-years old patient is reported who became ill with recurrent attacks of coughing and haemoptysis some 3 weeks before death. An extensive mediastinal tumor was demonstrated radiologically. Pathoanatomical examination showed a pleomorphic rhabdomyosarcoma of the lower left pulmonary lobe with metastases in the right lung and regional lymph nodes.", "contents": "[Pulmonary rhabdomyosarcoma (author's transl)]. A 55-years old patient is reported who became ill with recurrent attacks of coughing and haemoptysis some 3 weeks before death. An extensive mediastinal tumor was demonstrated radiologically. Pathoanatomical examination showed a pleomorphic rhabdomyosarcoma of the lower left pulmonary lobe with metastases in the right lung and regional lymph nodes."}
{"id": "PMID:1084061", "title": "[Analysis of two different surgical corrections of the coarctation of the aorta (follow up examination of 49 children) (author's transl)].", "content": "36 children operated upon with the diagnosis coarctation of the aorta have been controlled in a follow-up examination. We payed special attention to the surgical technique and brought the results in connection with the method of operation. One group had the stenosis corrected by resection and end-to-end-anastomosis, the second group with plastic operation, either by a longitudinal incision, resection of a diaphragm and transverse closure, or by improving the dilatation using a dacron patch. The results in both groups are satisfactory. Actually we prefer to carry out the resection with end-to-end anastomosis. Plastic correction remains reserved for selected cases. We believe that the high mortality rate in infants can be reduced by operation in hypothermy and by removing the stenosis as completely as possible.", "contents": "[Analysis of two different surgical corrections of the coarctation of the aorta (follow up examination of 49 children) (author's transl)]. 36 children operated upon with the diagnosis coarctation of the aorta have been controlled in a follow-up examination. We payed special attention to the surgical technique and brought the results in connection with the method of operation. One group had the stenosis corrected by resection and end-to-end-anastomosis, the second group with plastic operation, either by a longitudinal incision, resection of a diaphragm and transverse closure, or by improving the dilatation using a dacron patch. The results in both groups are satisfactory. Actually we prefer to carry out the resection with end-to-end anastomosis. Plastic correction remains reserved for selected cases. We believe that the high mortality rate in infants can be reduced by operation in hypothermy and by removing the stenosis as completely as possible."}
{"id": "PMID:1084064", "title": "[Nuclear structures at late stages of Rana temporaria oogenesis. II. Autoradiographic data].", "content": "Seasonal dynamics of RNA synthesis in the nuclei of 3rd year vitellogenetic oocytes of Rana temporaria has been studied by autoradiography. The nucleoli and chromosomes exhibit the highest level of RNA synthesis in summer, when lampbrush chromosomes are well developed, and multiple nucleoli start moving away from the nuclear membrane. The weakening of RNA synthesis is observed in autumn, when the nuclear structures assemble in the centre of the nucleus to form the karyosphere. The synthesis level is the lowest in winter, and increases in spring. The karyosphere of Rana temporaria is involved in protein metabolism of oocytes. Possible reasons of the seasonal RNA synthesis dynamics by the oocyte nuclei are discussed.", "contents": "[Nuclear structures at late stages of Rana temporaria oogenesis. II. Autoradiographic data]. Seasonal dynamics of RNA synthesis in the nuclei of 3rd year vitellogenetic oocytes of Rana temporaria has been studied by autoradiography. The nucleoli and chromosomes exhibit the highest level of RNA synthesis in summer, when lampbrush chromosomes are well developed, and multiple nucleoli start moving away from the nuclear membrane. The weakening of RNA synthesis is observed in autumn, when the nuclear structures assemble in the centre of the nucleus to form the karyosphere. The synthesis level is the lowest in winter, and increases in spring. The karyosphere of Rana temporaria is involved in protein metabolism of oocytes. Possible reasons of the seasonal RNA synthesis dynamics by the oocyte nuclei are discussed."}
{"id": "PMID:1084065", "title": "[Effects of Hodgkin cytotoxic serum on electrophoretic mobility of normal and Hodgkin peripheral blood lymphocytes. (author's transl)].", "content": "The effect of Hodgkin patient cytotoxic sera on the electrophoretic mobility of normal and Hodgkin peripheral blood allo-lymphocytes has been studied. Contact with cytotoxic serum determined a significant decrease in the electrophoretic mobility of lymphocytes, due to the presence of cytotoxic antibody on the lymphocyte surface. The antibody seems to be directed against T-lymphocytes. The results are discussed in the light of the preceding data by the authors on the role of anti-T-autoantibodies in Hodgkin's disease.", "contents": "[Effects of Hodgkin cytotoxic serum on electrophoretic mobility of normal and Hodgkin peripheral blood lymphocytes. (author's transl)]. The effect of Hodgkin patient cytotoxic sera on the electrophoretic mobility of normal and Hodgkin peripheral blood allo-lymphocytes has been studied. Contact with cytotoxic serum determined a significant decrease in the electrophoretic mobility of lymphocytes, due to the presence of cytotoxic antibody on the lymphocyte surface. The antibody seems to be directed against T-lymphocytes. The results are discussed in the light of the preceding data by the authors on the role of anti-T-autoantibodies in Hodgkin's disease."}
{"id": "PMID:1084068", "title": "Early acute aseptic iritis after cataract extraction.", "content": "Severe iritis which occurs within the first five days after cataract extraction may be categorized as (1) bacterial endophthalmitis, (2) toxic iritis, or (3) aseptic iritis. These entities can sometimes be distinguished because of their clinical features. If bacterial endophthalmitis is suspected, anterior chamber paracentesis should be considered and appropriate antibiotic treatment should be initiated. Acute iritis may result from the introduction of toxic agents into the eye, and may follow the use of products sterilized with ethylene oxide. Early acute aseptic iritis probably occurs more often than has previously been recognized. Response to intensive anti-inflammatory treatment is usually prompt and dramatic. The judicious use of cryoextraction and the careful manipulation of intraocular tissues may minimize the incidence and the severity of postoperative inflammation.", "contents": "Early acute aseptic iritis after cataract extraction. Severe iritis which occurs within the first five days after cataract extraction may be categorized as (1) bacterial endophthalmitis, (2) toxic iritis, or (3) aseptic iritis. These entities can sometimes be distinguished because of their clinical features. If bacterial endophthalmitis is suspected, anterior chamber paracentesis should be considered and appropriate antibiotic treatment should be initiated. Acute iritis may result from the introduction of toxic agents into the eye, and may follow the use of products sterilized with ethylene oxide. Early acute aseptic iritis probably occurs more often than has previously been recognized. Response to intensive anti-inflammatory treatment is usually prompt and dramatic. The judicious use of cryoextraction and the careful manipulation of intraocular tissues may minimize the incidence and the severity of postoperative inflammation."}
{"id": "PMID:1084076", "title": "[Electric stimulation of intestinal motility in the postoperative period].", "content": "The authors performed 208 electric stimulations of the bowel motor activity with the aim of prophylaxis and treatment of its postoperative paresis. The studies  have shown that electric stimulation accelerated considerably the process of rehabilitation of the bowel motor function, but it would not prevent the development of grave functional intestinal obstruction. But nevertheless  electric stimulation is a highly effective method in the complex of measures for treatment of grave functional intestinal obstruction. Electric stimulation of the bowel should be introduced in every day practice of intensive postoperative care departments.", "contents": "[Electric stimulation of intestinal motility in the postoperative period]. The authors performed 208 electric stimulations of the bowel motor activity with the aim of prophylaxis and treatment of its postoperative paresis. The studies  have shown that electric stimulation accelerated considerably the process of rehabilitation of the bowel motor function, but it would not prevent the development of grave functional intestinal obstruction. But nevertheless  electric stimulation is a highly effective method in the complex of measures for treatment of grave functional intestinal obstruction. Electric stimulation of the bowel should be introduced in every day practice of intensive postoperative care departments."}
{"id": "PMID:1084077", "title": "[Intestinal lesions after chemical burns of the digestive tract].", "content": "Based on the analysis of 235 patients with chemical burns of the esophagus and stomach and their sequelae, as well as the data of 111 pathoanatomical and medicolegal autopsies in persons, died in different periods after poisoning with corrosive substances, a burn of the duodenum and small intestine was observed in 71 cases. It was found that such factors as an intake of large doses of caustic liquors, poisoning in full stomach, especially in a state of alcohol intoxication, largely contribute to retropyloric penetration of a corrosive substance. A burn of the duodenum and small intestine would considerably aggravate the life prognosis during the first days of poisoning, and the former was observed in every second patient who died. In later period insignificant lesions disappeared completely, while at the site of deep burns an isolated or associated narrowing of the duodenum and small intestine developed. Surgical interventions were performed mainly in later periods in the presence of scarring contractions of the duodenum and small intestine, that impede an intestinal passage, and were aimed at its restoration. In the early period operations were performed only in life risk complications (intestinal hemorrhages, peritonitis), and these were of a palliative character.", "contents": "[Intestinal lesions after chemical burns of the digestive tract]. Based on the analysis of 235 patients with chemical burns of the esophagus and stomach and their sequelae, as well as the data of 111 pathoanatomical and medicolegal autopsies in persons, died in different periods after poisoning with corrosive substances, a burn of the duodenum and small intestine was observed in 71 cases. It was found that such factors as an intake of large doses of caustic liquors, poisoning in full stomach, especially in a state of alcohol intoxication, largely contribute to retropyloric penetration of a corrosive substance. A burn of the duodenum and small intestine would considerably aggravate the life prognosis during the first days of poisoning, and the former was observed in every second patient who died. In later period insignificant lesions disappeared completely, while at the site of deep burns an isolated or associated narrowing of the duodenum and small intestine developed. Surgical interventions were performed mainly in later periods in the presence of scarring contractions of the duodenum and small intestine, that impede an intestinal passage, and were aimed at its restoration. In the early period operations were performed only in life risk complications (intestinal hemorrhages, peritonitis), and these were of a palliative character."}
{"id": "PMID:1084083", "title": "[Irradiated cases of cervical and breast cancer II. Comparative investigation of the immune status of irradiated cases with stage III cancer of the cervix and operated, non-irradiated cases (author's transl)].", "content": "Eleven patients with stage-III cancer of the cervix were investigated before, during and after radio-therapy in regard to their state of humoral immunity on the basis of determinations of the serum IgG, IgM and IgA concentration, of hetero- and isoagglutinins, of tetanus antitoxin before and after vaccination with toxoid, of measles antibodies and of the percentage of lymphocyte membrane fluorescence. The cellular immunity of the same patients was investigated by determination of the percentage of spontaneously-rosetting lymphocytes, of skin-test reactivity with DNCB before and after sensitization, of skin-test reactivity with candida, trichophyton, varidase, OT and staphylo antigen. The function of polymorpho-nuclear leucocytes was investigated by means of the NVT test and St. aureus, E. coli and latex particles. All investigations were performed both before, and 3, 6, 9 and 12 weeks after the commencement of radiotherapy and the results were compared with those of an operated, non-irradiated group (stages I b and II a). Two types of noteworthy results were observed: 1. A decrease in immunological reactivity, probably in connection with cancer, since this reaction was observed both in irradiated and in non-irradiated cases, characterized by lowered or absent immune answer to tetanus toxoid, lymphopenia, decrease in sensitization to DNCB and less positive skin tests to old tuberculin and varidase. 2. An additional inhibition (although in one investigation stimulation of the immune answer was also seen), probably in connection with radiotherapy, characterized by an additional decrease in immune answer to tetanus toxoid, in skin sensitivity to DNCB sensitization and in tests with old tuberculin, and an augmented lymphopenia, as well as an increase in positive skin tests with varidase. No significant changes were observed with any other method.", "contents": "[Irradiated cases of cervical and breast cancer II. Comparative investigation of the immune status of irradiated cases with stage III cancer of the cervix and operated, non-irradiated cases (author's transl)]. Eleven patients with stage-III cancer of the cervix were investigated before, during and after radio-therapy in regard to their state of humoral immunity on the basis of determinations of the serum IgG, IgM and IgA concentration, of hetero- and isoagglutinins, of tetanus antitoxin before and after vaccination with toxoid, of measles antibodies and of the percentage of lymphocyte membrane fluorescence. The cellular immunity of the same patients was investigated by determination of the percentage of spontaneously-rosetting lymphocytes, of skin-test reactivity with DNCB before and after sensitization, of skin-test reactivity with candida, trichophyton, varidase, OT and staphylo antigen. The function of polymorpho-nuclear leucocytes was investigated by means of the NVT test and St. aureus, E. coli and latex particles. All investigations were performed both before, and 3, 6, 9 and 12 weeks after the commencement of radiotherapy and the results were compared with those of an operated, non-irradiated group (stages I b and II a). Two types of noteworthy results were observed: 1. A decrease in immunological reactivity, probably in connection with cancer, since this reaction was observed both in irradiated and in non-irradiated cases, characterized by lowered or absent immune answer to tetanus toxoid, lymphopenia, decrease in sensitization to DNCB and less positive skin tests to old tuberculin and varidase. 2. An additional inhibition (although in one investigation stimulation of the immune answer was also seen), probably in connection with radiotherapy, characterized by an additional decrease in immune answer to tetanus toxoid, in skin sensitivity to DNCB sensitization and in tests with old tuberculin, and an augmented lymphopenia, as well as an increase in positive skin tests with varidase. No significant changes were observed with any other method."}
{"id": "PMID:1084087", "title": "[ Histopathological changes in the thymus of the white mouse after infection with Toxoplasma gondii (author's transl)].", "content": "Three days after infection of mice with a virulent strain of T. gondii first histopathological destructions of the thymus are visible. The number of lymphocytes decreases step by step till to the animals' day of death on the 7th day p.i. At this time the cortex has lost all its thymocytes. Electronmicroscopical pictures show destruction of most of the reticulum cells and lymphocytes. In those cells which are still alive multiplicaiton of Toxoplasma trophozoites is to be seen. A lot of parasites are lying extracellular in the detritus of the destroyed thymus-cells. After infection of mice with an avirulent strain of T. gondii there is a loss of lymphocytes in the cortex of the thymus too. Starting at day 5th to 7th p.i. it reaches its peak at the time of the 10th to 15th day after infection. After this period restitution of lymphocytes in the cortex is going on. At about the 30th day p.i. replacement of all the lymphocytes is finished. Sometimes the cortex seems to be enlarged, that means now there are more thymocytes in the cortex than in uninfected controls. Neither reticulum cells nor lymphocytes show destruction of their ultrastructure. Only a process of activation of the lymphocytes can be seen by electronmicroscopy. In the lymphocytes the mitochondria are enlarged and there are more than in the controls. At the same time Golgi-apparatus and endoplasmatic reticulum become prominent.", "contents": "[ Histopathological changes in the thymus of the white mouse after infection with Toxoplasma gondii (author's transl)]. Three days after infection of mice with a virulent strain of T. gondii first histopathological destructions of the thymus are visible. The number of lymphocytes decreases step by step till to the animals' day of death on the 7th day p.i. At this time the cortex has lost all its thymocytes. Electronmicroscopical pictures show destruction of most of the reticulum cells and lymphocytes. In those cells which are still alive multiplicaiton of Toxoplasma trophozoites is to be seen. A lot of parasites are lying extracellular in the detritus of the destroyed thymus-cells. After infection of mice with an avirulent strain of T. gondii there is a loss of lymphocytes in the cortex of the thymus too. Starting at day 5th to 7th p.i. it reaches its peak at the time of the 10th to 15th day after infection. After this period restitution of lymphocytes in the cortex is going on. At about the 30th day p.i. replacement of all the lymphocytes is finished. Sometimes the cortex seems to be enlarged, that means now there are more thymocytes in the cortex than in uninfected controls. Neither reticulum cells nor lymphocytes show destruction of their ultrastructure. Only a process of activation of the lymphocytes can be seen by electronmicroscopy. In the lymphocytes the mitochondria are enlarged and there are more than in the controls. At the same time Golgi-apparatus and endoplasmatic reticulum become prominent."}
{"id": "PMID:1084093", "title": "[Simultaneous vaccination with BCG and measles vaccine: results of a controlled trial].", "content": "The main purpose of the controled assay on a combination of B.C.G. and measles vaccine was to ascertain whether these two vaccines could be administered on young children in simultaneous injections, in two different parts of the body and on the same day, and whether in that case they led to rates of haemagglutination inhibitory antibodies against measles on the one hand, and induration diameters of Mantoux's intradermoreactions against B.C.G. on the other, not inferior to rates and diameters obtained when using the same vaccines separately. This survey, carried out in Cameroon, involved one thousand individuals. It confirms the suggestion that the two vaccinations may be carried out simultaneously when circumstances require an early and total protection of a child population with minimum sanitary stress.", "contents": "[Simultaneous vaccination with BCG and measles vaccine: results of a controlled trial]. The main purpose of the controled assay on a combination of B.C.G. and measles vaccine was to ascertain whether these two vaccines could be administered on young children in simultaneous injections, in two different parts of the body and on the same day, and whether in that case they led to rates of haemagglutination inhibitory antibodies against measles on the one hand, and induration diameters of Mantoux's intradermoreactions against B.C.G. on the other, not inferior to rates and diameters obtained when using the same vaccines separately. This survey, carried out in Cameroon, involved one thousand individuals. It confirms the suggestion that the two vaccinations may be carried out simultaneously when circumstances require an early and total protection of a child population with minimum sanitary stress."}
{"id": "PMID:1084098", "title": "In vitro effect of metronidazole on the ultrastructure of Trichomonas vaginalis donn\u00e9.", "content": "The effect of metronidazole on the growth and fine structure of exponeentially growing cells of Trichomonas vaginalis was examined. Cell division stopped about one hour after the addition of 1-4 mug/ml of metronidazole, and the cells were apparently arrested in the interphase. One to two hours after the addition of metronidazole, the movement of flagella and of the undulating membrane had decreased, but cell death had not occurred until 7-8 hours later. The fine structure of the cytoplasm was changed 30 to 60 minutes after addition of metronidazole. Proportionally, the number of polyribosomes had decreased whereas the number of single ribosomes in the cytoplasm had increased. The electron-density of the cytoplasmic matrix was higher in cells which were treated with metronidazole than in cells from control cultures. The findings of the present study indicate that the initial effect of metronidazole on T. vag. cells in vitro is an inhibition of cell multiplication as well as an impairment of protein synthesis.", "contents": "In vitro effect of metronidazole on the ultrastructure of Trichomonas vaginalis donn\u00e9. The effect of metronidazole on the growth and fine structure of exponeentially growing cells of Trichomonas vaginalis was examined. Cell division stopped about one hour after the addition of 1-4 mug/ml of metronidazole, and the cells were apparently arrested in the interphase. One to two hours after the addition of metronidazole, the movement of flagella and of the undulating membrane had decreased, but cell death had not occurred until 7-8 hours later. The fine structure of the cytoplasm was changed 30 to 60 minutes after addition of metronidazole. Proportionally, the number of polyribosomes had decreased whereas the number of single ribosomes in the cytoplasm had increased. The electron-density of the cytoplasmic matrix was higher in cells which were treated with metronidazole than in cells from control cultures. The findings of the present study indicate that the initial effect of metronidazole on T. vag. cells in vitro is an inhibition of cell multiplication as well as an impairment of protein synthesis."}
{"id": "PMID:1084096", "title": "The origin of slow potentials in semicircular canals of the frog.", "content": "Slow potentials generated in the sensory organ of the ampulla were recorded in isolated semicircular canals of the frog by means of fluid electrodes. These potentials, which may be picked up from the intrampullar fluid and those from the ampullar nerve appear to be generated at different stages of the process taking place in crista ampullaris. Slow intra-ampullar potentials apparently reflect receptor potentials of hair cells. They are preserved after degeneration of the nerve fibre endings and are relatively insensitive to DNP poisoning; their amplitude is maximum at high K+ concentrations. Slow nerve potentials appear to be due to electronic spreading of post-synaptic excitatory potentials generated at cytoneural junctions. They disappear after degeneration of the nerve fibres, in low Ca++ and high Mg++ solutions and are extremely sensitive to DNP poisoning. An analysis of the time-course of the slow ampullar and nerve potentials referred to the discharge of impulses in afferent fibres was performed with a view to interpreting the transduction mechanism of semicircular canals.", "contents": "The origin of slow potentials in semicircular canals of the frog. Slow potentials generated in the sensory organ of the ampulla were recorded in isolated semicircular canals of the frog by means of fluid electrodes. These potentials, which may be picked up from the intrampullar fluid and those from the ampullar nerve appear to be generated at different stages of the process taking place in crista ampullaris. Slow intra-ampullar potentials apparently reflect receptor potentials of hair cells. They are preserved after degeneration of the nerve fibre endings and are relatively insensitive to DNP poisoning; their amplitude is maximum at high K+ concentrations. Slow nerve potentials appear to be due to electronic spreading of post-synaptic excitatory potentials generated at cytoneural junctions. They disappear after degeneration of the nerve fibres, in low Ca++ and high Mg++ solutions and are extremely sensitive to DNP poisoning. An analysis of the time-course of the slow ampullar and nerve potentials referred to the discharge of impulses in afferent fibres was performed with a view to interpreting the transduction mechanism of semicircular canals."}
{"id": "PMID:1084095", "title": "A reexamination of \"neck reflex\" eye movements in the rabbit.", "content": "Horizontal eye movements of rabbits were recorded during sinusoidal oscillation (15-40 degrees, pk-pk; 0.1-8 Hz) and step displacement of the body in yaw about a fixed head. Modulated, slow-phase eye movements followed all frequencies of stimulus with relatively invariant amplitudes (2-4 degrees). Saccadic movements, up to 17 degrees, accompanied all frequencies of oscillation and particularly step displacements. Saccadic amplitude was unrelated to measurable characteristics of the stimuli but was a function of arousal. The latency of any eye movement was a minimum of 80 msec. It is concluded that none of the observed eye movements provide stabilization during head movements but are evidence of the contribution of neck information to general mechanisms of whole body orientation.", "contents": "A reexamination of \"neck reflex\" eye movements in the rabbit. Horizontal eye movements of rabbits were recorded during sinusoidal oscillation (15-40 degrees, pk-pk; 0.1-8 Hz) and step displacement of the body in yaw about a fixed head. Modulated, slow-phase eye movements followed all frequencies of stimulus with relatively invariant amplitudes (2-4 degrees). Saccadic movements, up to 17 degrees, accompanied all frequencies of oscillation and particularly step displacements. Saccadic amplitude was unrelated to measurable characteristics of the stimuli but was a function of arousal. The latency of any eye movement was a minimum of 80 msec. It is concluded that none of the observed eye movements provide stabilization during head movements but are evidence of the contribution of neck information to general mechanisms of whole body orientation."}
{"id": "PMID:1084101", "title": "Comparison of skin test results obtained with different dust, Dermatophagoides; Acarus siro and spider extracts.", "content": "The authors describe the comparative analysis of skin tests performed in 87 asthmatic children with extracts of house dust, grain dust and different arthropodes beloging to the Arachidaegenus. Most of these patients were living in traditional agricultural environment. Reactions to the Acarus siro and grain dust (Sevac) extracts gave significant correlation, whereas house dust did not show this agreement. Extracts from spiders (Aranidae) caused often, in 40% of asthmatic children, positive skin reaction; that was the same frequency as with Dermatophagoides, nevertheless the correlation was only partial. The authours conclude that Acarus siro and antigenically related mites are the main allergizing component of grain dust; excretes of spiders display a comparable allergizing factor in house dust as do Pyroglyphid mites in this area.", "contents": "Comparison of skin test results obtained with different dust, Dermatophagoides; Acarus siro and spider extracts. The authors describe the comparative analysis of skin tests performed in 87 asthmatic children with extracts of house dust, grain dust and different arthropodes beloging to the Arachidaegenus. Most of these patients were living in traditional agricultural environment. Reactions to the Acarus siro and grain dust (Sevac) extracts gave significant correlation, whereas house dust did not show this agreement. Extracts from spiders (Aranidae) caused often, in 40% of asthmatic children, positive skin reaction; that was the same frequency as with Dermatophagoides, nevertheless the correlation was only partial. The authours conclude that Acarus siro and antigenically related mites are the main allergizing component of grain dust; excretes of spiders display a comparable allergizing factor in house dust as do Pyroglyphid mites in this area."}
{"id": "PMID:1084105", "title": "Use of problem-oriented medical record in psychiatry: a survey of university-based residency training programs.", "content": "A survey of university-based psychiatric residency training programs revealed that nearly one-half of them use the problem-oriented medical record (POMR) and that conversion to this system appears to be growing rapidly. Experiences with the method have in general been positive. The author identifies factors associated with successful operation of the POMR and suggests that an integrated, hospital-wide adoption of the system may be crucial.", "contents": "Use of problem-oriented medical record in psychiatry: a survey of university-based residency training programs. A survey of university-based psychiatric residency training programs revealed that nearly one-half of them use the problem-oriented medical record (POMR) and that conversion to this system appears to be growing rapidly. Experiences with the method have in general been positive. The author identifies factors associated with successful operation of the POMR and suggests that an integrated, hospital-wide adoption of the system may be crucial."}
{"id": "PMID:1084108", "title": "Analysis of tasks and human factors in anesthesia for coronary-artery bypass.", "content": "A comprehensive task analysis of the anesthetist's activities during coronary-artery bypass procedures was carried out using time-lapse cinematography. This film was analyzed frame by frame to detect types of activities and the proportion of time spent on each. Link analysis was used to reveal recurring sequences of activities. Of the 9 activity categories, the anesthetist spends the greatest portion of time observing the patient and scanning the entire operative and monitoring field. Ten to 15% of the time is spent logging data on the anesthetic record. The challenge for the anesthetist is to optimize his performance, and those under his direction, in the face of the changing workload resulting from variations in patients' conditions. Use of an automated monitoring system that charts patient vital signs would allow the anesthetist to apportion this time among other tasks.", "contents": "Analysis of tasks and human factors in anesthesia for coronary-artery bypass. A comprehensive task analysis of the anesthetist's activities during coronary-artery bypass procedures was carried out using time-lapse cinematography. This film was analyzed frame by frame to detect types of activities and the proportion of time spent on each. Link analysis was used to reveal recurring sequences of activities. Of the 9 activity categories, the anesthetist spends the greatest portion of time observing the patient and scanning the entire operative and monitoring field. Ten to 15% of the time is spent logging data on the anesthetic record. The challenge for the anesthetist is to optimize his performance, and those under his direction, in the face of the changing workload resulting from variations in patients' conditions. Use of an automated monitoring system that charts patient vital signs would allow the anesthetist to apportion this time among other tasks."}
{"id": "PMID:1084109", "title": "Nitrous oxide encephalography: 5-year experience with 475 pediatric patients.", "content": "475 pediatric patients with a variety of neurologic conditions were subjected to encephalography, using N2O as the contrast medium and N2O-halothane as anesthetic agents. This technic is both safe and effective, as reflected by no mortality and satisfactory roentgenograms. By application of the principles of gas physics, postoperative discomfort has been greatly reduced, resulting in a more comfortable convalescence and earlier discharge.", "contents": "Nitrous oxide encephalography: 5-year experience with 475 pediatric patients. 475 pediatric patients with a variety of neurologic conditions were subjected to encephalography, using N2O as the contrast medium and N2O-halothane as anesthetic agents. This technic is both safe and effective, as reflected by no mortality and satisfactory roentgenograms. By application of the principles of gas physics, postoperative discomfort has been greatly reduced, resulting in a more comfortable convalescence and earlier discharge."}
{"id": "PMID:1084115", "title": "The 9p- syndrome.", "content": "Six patients (4 females and 2 males) with terminal deletion of the short arm of chromosome 9 distal to band p22 are described. The disorder constitutes a clinically identifiable syndrome consisting of mental retardation, sociable personality, trigonocephaly, mongoloid eyes, wide flat nasal bridge, anteverted nostrils, long upper lip, short neck, long digits mostly secondary to long middle phalanges, and predominance of whorls on fingers. The findings suggest that the clinical features are antithetical to the trisomy 9p syndrome. The deleted chromosome segment is relatively small and could be easily overlooked. It is hoped that this delineation of clinical features seen in 9,p- patients may help in focusing attention on the small deletion.", "contents": "The 9p- syndrome. Six patients (4 females and 2 males) with terminal deletion of the short arm of chromosome 9 distal to band p22 are described. The disorder constitutes a clinically identifiable syndrome consisting of mental retardation, sociable personality, trigonocephaly, mongoloid eyes, wide flat nasal bridge, anteverted nostrils, long upper lip, short neck, long digits mostly secondary to long middle phalanges, and predominance of whorls on fingers. The findings suggest that the clinical features are antithetical to the trisomy 9p syndrome. The deleted chromosome segment is relatively small and could be easily overlooked. It is hoped that this delineation of clinical features seen in 9,p- patients may help in focusing attention on the small deletion."}
{"id": "PMID:1084116", "title": "[Partial trisomy 15q due to maternal translocation t(7;15)(q35;14)].", "content": "Partial trisomy for the long arm of chromosome 15 was detected in a 21-year-old girl with severe growth and mental retardation. A balanced reciprocal translocation - t(7;15)(q35;q14) - is present in the mother.", "contents": "[Partial trisomy 15q due to maternal translocation t(7;15)(q35;14)]. Partial trisomy for the long arm of chromosome 15 was detected in a 21-year-old girl with severe growth and mental retardation. A balanced reciprocal translocation - t(7;15)(q35;q14) - is present in the mother."}
{"id": "PMID:1084117", "title": "Trisomy 19 q.", "content": "Two sibs with trisomy for the long arm of chromosome 19 are reported. The common features included flat facial profile with microcephaly, hypertelorism, ptosis, prominence of the glabella, small nose with anteverted nostrils and a characteristic fish-shaped mouth. In addition congenital heart disease, physical retardation and seizures were seen in both sibs. That tristomy 19q can be suspected clinically is emphasized.", "contents": "Trisomy 19 q. Two sibs with trisomy for the long arm of chromosome 19 are reported. The common features included flat facial profile with microcephaly, hypertelorism, ptosis, prominence of the glabella, small nose with anteverted nostrils and a characteristic fish-shaped mouth. In addition congenital heart disease, physical retardation and seizures were seen in both sibs. That tristomy 19q can be suspected clinically is emphasized."}
{"id": "PMID:1084118", "title": "[Attempt at antenatal diagnosis of argininosuccinic aciduria].", "content": "Antenatal diagnosis in a woman previously delivered of a child with arginino-succinic aciduria, due to deficiency of arginino-succinase (EC 4.3.2.1.), showed the fetus to be unaffected. This was confirmed at birth.", "contents": "[Attempt at antenatal diagnosis of argininosuccinic aciduria]. Antenatal diagnosis in a woman previously delivered of a child with arginino-succinic aciduria, due to deficiency of arginino-succinase (EC 4.3.2.1.), showed the fetus to be unaffected. This was confirmed at birth."}
{"id": "PMID:1084119", "title": "[Distribution of chromosome breakage point induced by arabinosylcytosine on in-vitro human lymphocytes].", "content": "The cytogenetic effects of arabinosyl cytosine on human leukocytes in vitro has been studied using continuous and discontinuous treatments. Optimum activity occurs at the end of the S period. Interchromosomal distribution of breaks is non-random. The hypothesis of a very late replication for some chromatid segments is discussed. The relations between banding and the position of breaks are discussed.", "contents": "[Distribution of chromosome breakage point induced by arabinosylcytosine on in-vitro human lymphocytes]. The cytogenetic effects of arabinosyl cytosine on human leukocytes in vitro has been studied using continuous and discontinuous treatments. Optimum activity occurs at the end of the S period. Interchromosomal distribution of breaks is non-random. The hypothesis of a very late replication for some chromatid segments is discussed. The relations between banding and the position of breaks are discussed."}
{"id": "PMID:1084120", "title": "[Partial 7 q trisomy. One or 2 syndromes? Apropos of a new case].", "content": "Partial trisomy 7qter due to malsegregation of a familial balanced translocation t(7;8)(q33;p113) is reported in a 2-year-old boy with the following features: psychomotor retardation (IQ = 46); hypotonia; normal; facial asymetry with palpebral fissures slanted downwards and outwards, deeply set eyes, and divergent strabismus.", "contents": "[Partial 7 q trisomy. One or 2 syndromes? Apropos of a new case]. Partial trisomy 7qter due to malsegregation of a familial balanced translocation t(7;8)(q33;p113) is reported in a 2-year-old boy with the following features: psychomotor retardation (IQ = 46); hypotonia; normal; facial asymetry with palpebral fissures slanted downwards and outwards, deeply set eyes, and divergent strabismus."}
{"id": "PMID:1084121", "title": "De novo appearance of a translocation t(5p; 2Iq), and its transmission in both balanced and unbalanced forms to the next generation.", "content": "A family is described in which a reciprocal translocation involving 5p and 21q appeared de novo in the chromosome complement of a woman who then transmitted it in both balanced and unbalanced form to her progeny. The proposita, a child with the cri du chat syndrome, had a deficiency for most of 5p, all of 21p, 21 centromere, and a small proximal segment of 21q. The reported cases of the cri du chat syndrome associated with translocations are reviewed and discussed in relation to this family.", "contents": "De novo appearance of a translocation t(5p; 2Iq), and its transmission in both balanced and unbalanced forms to the next generation. A family is described in which a reciprocal translocation involving 5p and 21q appeared de novo in the chromosome complement of a woman who then transmitted it in both balanced and unbalanced form to her progeny. The proposita, a child with the cri du chat syndrome, had a deficiency for most of 5p, all of 21p, 21 centromere, and a small proximal segment of 21q. The reported cases of the cri du chat syndrome associated with translocations are reviewed and discussed in relation to this family."}
{"id": "PMID:1084122", "title": "Chromosome breakage in low birth weight newborns.", "content": "The frequency of chromosome aberrations in cells cultured from umbilical cord blood was determined for 50 low birth weight (LBW) and 50 normal birth weight (NBW) euploid newborns matched for sex, race, and maternal age. The metaphase spreads had been prepared in the course of an earlier study of frequency of aneuploidy and results are from 72-h cultures, i.e., presumably, at the second division in vitro. There were no significant differences between the two groups in the frequency of cells with chromosome breakage, chromosome gaps, or hyperdiploid cells. There was, however, a significantly higher frequency of hypodiploid cells in the LBW group. The present findings differ from those of others who have reported an increase in chromosome breakage in premature newborns.", "contents": "Chromosome breakage in low birth weight newborns. The frequency of chromosome aberrations in cells cultured from umbilical cord blood was determined for 50 low birth weight (LBW) and 50 normal birth weight (NBW) euploid newborns matched for sex, race, and maternal age. The metaphase spreads had been prepared in the course of an earlier study of frequency of aneuploidy and results are from 72-h cultures, i.e., presumably, at the second division in vitro. There were no significant differences between the two groups in the frequency of cells with chromosome breakage, chromosome gaps, or hyperdiploid cells. There was, however, a significantly higher frequency of hypodiploid cells in the LBW group. The present findings differ from those of others who have reported an increase in chromosome breakage in premature newborns."}
{"id": "PMID:1084123", "title": "Deletion - translocation del (12) (p11) leads to (t10;12) (p13;pII).", "content": "Renewed examinatinon with improved banding techniques of a boy previously reported to have the karyotype 46, XY,del(12)(p11) revealed a translocation 46, XY,t(10;12)(p13;p11), and reexamination of a boy previously reported to have the karyotype 46,XY/46,XY,del(5)(p13) showed the same mosaicism, but with a significantly lower frequency of cells with del(5)(p13), 8% compared with 23% at the time of birth. The decrease of the frequency of cells with chromosome abnormality in mixoploids during the first years of life as found in the present case as well as in prevously reported cases is discussed.", "contents": "Deletion - translocation del (12) (p11) leads to (t10;12) (p13;pII). Renewed examinatinon with improved banding techniques of a boy previously reported to have the karyotype 46, XY,del(12)(p11) revealed a translocation 46, XY,t(10;12)(p13;p11), and reexamination of a boy previously reported to have the karyotype 46,XY/46,XY,del(5)(p13) showed the same mosaicism, but with a significantly lower frequency of cells with del(5)(p13), 8% compared with 23% at the time of birth. The decrease of the frequency of cells with chromosome abnormality in mixoploids during the first years of life as found in the present case as well as in prevously reported cases is discussed."}
{"id": "PMID:1084124", "title": "Trisomy iop.", "content": "A stillborn male fetus having a trisomy of the short arm of chromosome No 10 is described. The father is a carrier of the reciprocal translocation 46XY,t(10;21) (10pter leads to 10p11::21p11 leads to 21qter). The clinical picture included growth retardation, bilateral cleft lip and palate, micrognathia, short neck, microphalus and bilateral clubbed feet. The long bones were markedly thinned with spontaneous fractures. Autopsy findings included pulmonary hypoplasia and renal dysplasia. Previous reports of trisomy 10 and trisomy of the short arm of chromosome 10 are discussed.", "contents": "Trisomy iop. A stillborn male fetus having a trisomy of the short arm of chromosome No 10 is described. The father is a carrier of the reciprocal translocation 46XY,t(10;21) (10pter leads to 10p11::21p11 leads to 21qter). The clinical picture included growth retardation, bilateral cleft lip and palate, micrognathia, short neck, microphalus and bilateral clubbed feet. The long bones were markedly thinned with spontaneous fractures. Autopsy findings included pulmonary hypoplasia and renal dysplasia. Previous reports of trisomy 10 and trisomy of the short arm of chromosome 10 are discussed."}
{"id": "PMID:1084125", "title": "[Trisomy 10 p. A previously reported case explained by binding].", "content": "A previously reported male infant having died at 4 months, was considered trisomic Cp, his mother being carrier of balanced translocation t(Cp-;Bq +). Reexamination of the chromosome complement after R-banding showed the translocation to be t(4;10)(q35;p11). The propositus was therefore trisomic 10p. The essential clinical features were: a small birth weight and a short birth length; hypotonia; psychomotor retardation; dolichocephalia; a high and bulky forehead; narrow lips; large, lowset ears with a posterior rotation; a small chin; bone and joint anomalies; dextrocardia.", "contents": "[Trisomy 10 p. A previously reported case explained by binding]. A previously reported male infant having died at 4 months, was considered trisomic Cp, his mother being carrier of balanced translocation t(Cp-;Bq +). Reexamination of the chromosome complement after R-banding showed the translocation to be t(4;10)(q35;p11). The propositus was therefore trisomic 10p. The essential clinical features were: a small birth weight and a short birth length; hypotonia; psychomotor retardation; dolichocephalia; a high and bulky forehead; narrow lips; large, lowset ears with a posterior rotation; a small chin; bone and joint anomalies; dextrocardia."}
{"id": "PMID:1084126", "title": "[Trisomy 11 q (q23.1 - qter) through maternal translocation t(11;22) (q23.1;q11.1). A new case].", "content": "A 4-year-old boy with partial trisomy 11q resulting from malsegregation of a maternal translocation, t(11;22)(q23.1;q11.1), exhibits the following malformations: severe mental deficiency; growth retardation and hypotonia; brachycephaly with flattened occiput and forehead; facial dysmorphia; pre-auricular fistula. These features are in good agreement with the syndrome recently described for partial trisomy 11q. The translocation appears to be identical in that in three other families already reported.", "contents": "[Trisomy 11 q (q23.1 - qter) through maternal translocation t(11;22) (q23.1;q11.1). A new case]. A 4-year-old boy with partial trisomy 11q resulting from malsegregation of a maternal translocation, t(11;22)(q23.1;q11.1), exhibits the following malformations: severe mental deficiency; growth retardation and hypotonia; brachycephaly with flattened occiput and forehead; facial dysmorphia; pre-auricular fistula. These features are in good agreement with the syndrome recently described for partial trisomy 11q. The translocation appears to be identical in that in three other families already reported."}
{"id": "PMID:1084129", "title": "[Investigations of the T and B cells and the hemopexin-carrying lymphocytes in cases of porphyria cutanea tarda (author's transl)].", "content": "The immunoglobulins and hemopexin carrying cells have been determined in 14 patients having Porphyria cutanea tarda and in 15 healthy volunteers. At the same time, the stimulating effect of phytohemaglutinine and the leucocytesmigration inhibition by purified tuberculine proteins, were measured. In the 14 cases of Porphyria cutanea tarda, the IgG content and the number of hemopexin carrying lymphocytes was significantly increased, while the amounts of the other immunoglobuline carrying cells, and of T lymphocytes, was unchanged. Phytohemaglutinine stimulation and inhibition of leukocytes migration by purified tuberculine proteins were found to be identical in both patients and control groups.", "contents": "[Investigations of the T and B cells and the hemopexin-carrying lymphocytes in cases of porphyria cutanea tarda (author's transl)]. The immunoglobulins and hemopexin carrying cells have been determined in 14 patients having Porphyria cutanea tarda and in 15 healthy volunteers. At the same time, the stimulating effect of phytohemaglutinine and the leucocytesmigration inhibition by purified tuberculine proteins, were measured. In the 14 cases of Porphyria cutanea tarda, the IgG content and the number of hemopexin carrying lymphocytes was significantly increased, while the amounts of the other immunoglobuline carrying cells, and of T lymphocytes, was unchanged. Phytohemaglutinine stimulation and inhibition of leukocytes migration by purified tuberculine proteins were found to be identical in both patients and control groups."}
{"id": "PMID:1084127", "title": "Immunoglobulin binding factor (IBF) produced by T cells: selective interaction with slow migrating rabbit IgC.", "content": "Antigen-stimulated T lymphocytes release a variety of soluble factors among which an Immunoglobulin Binding Factor (IBF). We report a method of titration of IBF making use of its capacity to inhibit complement-dependent hemolysis of sheep erythrocytes sensitized with rabbit IgG antibodies. Using this technique we have investigated the specificity of IBG for rabbit immunoglobulins fractionated by DEAE-cellulose chromatography. The interaction of IBF with IgG is found to be restricted to the slowest migrating fractions of rabbit IgG.", "contents": "Immunoglobulin binding factor (IBF) produced by T cells: selective interaction with slow migrating rabbit IgC. Antigen-stimulated T lymphocytes release a variety of soluble factors among which an Immunoglobulin Binding Factor (IBF). We report a method of titration of IBF making use of its capacity to inhibit complement-dependent hemolysis of sheep erythrocytes sensitized with rabbit IgG antibodies. Using this technique we have investigated the specificity of IBG for rabbit immunoglobulins fractionated by DEAE-cellulose chromatography. The interaction of IBF with IgG is found to be restricted to the slowest migrating fractions of rabbit IgG."}
{"id": "PMID:1084131", "title": "Transformation of human lymphocytes in vitro by autologous and allogeneic rheumatoid synovial fluids.", "content": "To assess the possible participation of cellular immune mechanisms in the pathogenesis of rheumatoid arthritis (RA) in vitro studies of the blastogenicity of rheumatoid and non-rheumatoid synovial fluids for human peripheral blood lymphocytes were conducted. In autologous cultures it was found that 13 of 19 rheumatoid fluids induced significant lymphocyte blastogenesis, whereas only 1 of 13 nonrheumatoid fluids induced such a response. In allogeneic cultures rheumatoid fluids induce significant blastogenesis of RA lymphocytes in 18 of 23 experiments, and of non-RA lymphocytes in 8 of 18 experiments. By contrast, nonrheumatoid fluids induced significant blastogenesis of RA lymphocytes in 2 of 13 experiments, and of non-RA lymphocytes in 1 of 14 experiments. The blastogenicity of fluids was found to correlate significantly with the presence therein of immunofluorescent intracellular inclusions of immunoglobulin and complement. These studies support the concept that the presence of immune complexes in the majority of rheumatoid synovial fluids might render the latter blastogenic for human lymphocytes in vivo, thereby perpetuating rheumatoid synovitis.", "contents": "Transformation of human lymphocytes in vitro by autologous and allogeneic rheumatoid synovial fluids. To assess the possible participation of cellular immune mechanisms in the pathogenesis of rheumatoid arthritis (RA) in vitro studies of the blastogenicity of rheumatoid and non-rheumatoid synovial fluids for human peripheral blood lymphocytes were conducted. In autologous cultures it was found that 13 of 19 rheumatoid fluids induced significant lymphocyte blastogenesis, whereas only 1 of 13 nonrheumatoid fluids induced such a response. In allogeneic cultures rheumatoid fluids induce significant blastogenesis of RA lymphocytes in 18 of 23 experiments, and of non-RA lymphocytes in 8 of 18 experiments. By contrast, nonrheumatoid fluids induced significant blastogenesis of RA lymphocytes in 2 of 13 experiments, and of non-RA lymphocytes in 1 of 14 experiments. The blastogenicity of fluids was found to correlate significantly with the presence therein of immunofluorescent intracellular inclusions of immunoglobulin and complement. These studies support the concept that the presence of immune complexes in the majority of rheumatoid synovial fluids might render the latter blastogenic for human lymphocytes in vivo, thereby perpetuating rheumatoid synovitis."}
{"id": "PMID:1084128", "title": "[Cold lymphocytotoxins: their relationship with various physiological and pathological conditions].", "content": "The significance of cold lymphocytotoxins, observed at 15 degreesC, is not clearly understood at the present time. The frequency of their appearance has been studied in normal subjects (blood donors, aged people, vaccinated subjects, post-traumatic splenectomy) and in patients with a neurologic disease (multiple sclerosis), a neoplasic disease (breast cancer)and hematologic diseases (thrombocytopenia, acute leukemia, chronic lymphatic leukemia, Hodgkin disease and systemic lupus erythematosus). There are no antibodies found in the geriatric group; they are found only in 3,9 % of blood donors and in 18 % of the subjects after vaccinations. A range of 17 to 30 % is found in subjects with breast cancer or multiple sclerosis. More than 50 % of the individuals with Hodgkin disease or lupus erythematosus produce these antibodies (52 % and 73 % respectively). In acute leukemias and chronic lymphatic leukemias, lymphocytotoxic antibodies sometimes appear at 37 degrees, reacting with autologous cells and having no HL-A specificity.", "contents": "[Cold lymphocytotoxins: their relationship with various physiological and pathological conditions]. The significance of cold lymphocytotoxins, observed at 15 degreesC, is not clearly understood at the present time. The frequency of their appearance has been studied in normal subjects (blood donors, aged people, vaccinated subjects, post-traumatic splenectomy) and in patients with a neurologic disease (multiple sclerosis), a neoplasic disease (breast cancer)and hematologic diseases (thrombocytopenia, acute leukemia, chronic lymphatic leukemia, Hodgkin disease and systemic lupus erythematosus). There are no antibodies found in the geriatric group; they are found only in 3,9 % of blood donors and in 18 % of the subjects after vaccinations. A range of 17 to 30 % is found in subjects with breast cancer or multiple sclerosis. More than 50 % of the individuals with Hodgkin disease or lupus erythematosus produce these antibodies (52 % and 73 % respectively). In acute leukemias and chronic lymphatic leukemias, lymphocytotoxic antibodies sometimes appear at 37 degrees, reacting with autologous cells and having no HL-A specificity."}
{"id": "PMID:1084134", "title": "Early and late results after surgical treatment of preinfarction angina.", "content": "Among 3,707 patients who underwent aortocoronary bypass, 302 had preinfarction angina. Coronary angiography revealed single-vessel disease in 43 patients, double-vessel disease in 81, and triple in 178 patients. Plane ventriculography showed contractility to be normal in 178 patients, fair in 88, and poor in 36 patients. Left ventricular end-diastolic pressure was normal in 203 patients, 13 to 23 mm Hg in 73, and larger than or equal to 24 mm Hg in 26 patients. Using cardiopulmonary bypass and moderate hypothermia, single coronary bypass was performed in 45 patients, double bypass in 120 patients, triple in 118 patients, quadruple in 15, and quintuple in 4 patients. Right coronary artery endarterectomy was necessary in 22 patients. The early mortality was 6.6% (20 patients) and was strongly related to poor contractility and congestive heart failure. One- to four-year follow-up data were obtained in 126 patients. Late myocardial infarction occurred in 11 patients and caused 4 late deaths; 3 unrelated deaths occurred. Ten patients experienced no benefit from their operations, 56 are completely asymptomatic, and 53 are significantly improved. Our results show that surgical intervention can improve the poor prognosis of preinfarction angina and appears to be superior to medical treatment.", "contents": "Early and late results after surgical treatment of preinfarction angina. Among 3,707 patients who underwent aortocoronary bypass, 302 had preinfarction angina. Coronary angiography revealed single-vessel disease in 43 patients, double-vessel disease in 81, and triple in 178 patients. Plane ventriculography showed contractility to be normal in 178 patients, fair in 88, and poor in 36 patients. Left ventricular end-diastolic pressure was normal in 203 patients, 13 to 23 mm Hg in 73, and larger than or equal to 24 mm Hg in 26 patients. Using cardiopulmonary bypass and moderate hypothermia, single coronary bypass was performed in 45 patients, double bypass in 120 patients, triple in 118 patients, quadruple in 15, and quintuple in 4 patients. Right coronary artery endarterectomy was necessary in 22 patients. The early mortality was 6.6% (20 patients) and was strongly related to poor contractility and congestive heart failure. One- to four-year follow-up data were obtained in 126 patients. Late myocardial infarction occurred in 11 patients and caused 4 late deaths; 3 unrelated deaths occurred. Ten patients experienced no benefit from their operations, 56 are completely asymptomatic, and 53 are significantly improved. Our results show that surgical intervention can improve the poor prognosis of preinfarction angina and appears to be superior to medical treatment."}
{"id": "PMID:1084135", "title": "Intraaortic balloon assistance.", "content": "Intraaortic balloon (IAB) assistance in 64 patients over 2 1/2 years has resulted in a survival rate of 11% (1 patient) when used alone but 47% when utilized in patients treated surgically (long-term survival, 38% [21 patients]). Patients undergoing coronary artery bypass grafting or aortic valve replacement have a long-term survival of 50% (8 and 9 patients, respectively). The required duration of IAB support has a bearing on the clinical result. Complications have been minimal. Though it was originally developed to assist in the nonoperative management of complications of ischemic heart disease, IAB assistance offers significant promise as an adjuvant to operative therapy for both ischemic and valvular heart disease.", "contents": "Intraaortic balloon assistance. Intraaortic balloon (IAB) assistance in 64 patients over 2 1/2 years has resulted in a survival rate of 11% (1 patient) when used alone but 47% when utilized in patients treated surgically (long-term survival, 38% [21 patients]). Patients undergoing coronary artery bypass grafting or aortic valve replacement have a long-term survival of 50% (8 and 9 patients, respectively). The required duration of IAB support has a bearing on the clinical result. Complications have been minimal. Though it was originally developed to assist in the nonoperative management of complications of ischemic heart disease, IAB assistance offers significant promise as an adjuvant to operative therapy for both ischemic and valvular heart disease."}
{"id": "PMID:1084136", "title": "Local deep hypothermia for combined valvular and coronary heart disease.", "content": "Local deep hypothermia was utilized as the sole method of myocardial protection in 113 consecutive aortic valve replacements. Thirty-six patients had simultaneous revascularization, mitral valve replacement, tricuspid valve replacement, or a combination of these. In follow-up for as long as 36 months there have been 3 hospital deaths (2.6%), all occurring in the group having isolated aortic valve replacement. No hospital deaths occurred among patients undergoing a combined procedure.", "contents": "Local deep hypothermia for combined valvular and coronary heart disease. Local deep hypothermia was utilized as the sole method of myocardial protection in 113 consecutive aortic valve replacements. Thirty-six patients had simultaneous revascularization, mitral valve replacement, tricuspid valve replacement, or a combination of these. In follow-up for as long as 36 months there have been 3 hospital deaths (2.6%), all occurring in the group having isolated aortic valve replacement. No hospital deaths occurred among patients undergoing a combined procedure."}
{"id": "PMID:1084137", "title": "Experience with the radial artery graft for coronary artery bypass.", "content": "Forty-eight radial artery grafts and 22 saphenous vein grafts in 37 patients undergoing coronary bypass procedures were studied early postoperatively. Most of the saphenous vein grafts were patent, but one-half of the radial artery grafts were occluded. Failure of the radial artery grafts could not be attributed to unfavorable runoff in the recipient vessels. Radial arteries carrying higher flows and those to vessels having more severe degrees of proximal stenosis occluded with a higher frequency. The radial artery should not be used for coronary bypass.", "contents": "Experience with the radial artery graft for coronary artery bypass. Forty-eight radial artery grafts and 22 saphenous vein grafts in 37 patients undergoing coronary bypass procedures were studied early postoperatively. Most of the saphenous vein grafts were patent, but one-half of the radial artery grafts were occluded. Failure of the radial artery grafts could not be attributed to unfavorable runoff in the recipient vessels. Radial arteries carrying higher flows and those to vessels having more severe degrees of proximal stenosis occluded with a higher frequency. The radial artery should not be used for coronary bypass."}
{"id": "PMID:1084138", "title": "In favor of the Y-graft for aortocoronary bypass.", "content": "The technique of multiple coronary artery bypass grafting has included separate conduits, snake grafts, and Y-grafts. Against the Y-graft have been reports of lesser patency and several theoretical objections. The theory is discussed and a laboratory model presented which demonstrates a flow difference of 2 ml/5 sec or less between Y-grafts and single grafts. In conjunction with this, 171 patients having aortocoronary bypass were analyzed and showed a combined one-year graft patency of 77% with no significant difference between single grafts and Y-grafts. It is concluded that the technical simplicity of Y-grafts is advantageous and that, if the operation is performed within the guidelines set forth, revascularization with Y-grafts is an acceptable method.", "contents": "In favor of the Y-graft for aortocoronary bypass. The technique of multiple coronary artery bypass grafting has included separate conduits, snake grafts, and Y-grafts. Against the Y-graft have been reports of lesser patency and several theoretical objections. The theory is discussed and a laboratory model presented which demonstrates a flow difference of 2 ml/5 sec or less between Y-grafts and single grafts. In conjunction with this, 171 patients having aortocoronary bypass were analyzed and showed a combined one-year graft patency of 77% with no significant difference between single grafts and Y-grafts. It is concluded that the technical simplicity of Y-grafts is advantageous and that, if the operation is performed within the guidelines set forth, revascularization with Y-grafts is an acceptable method."}
{"id": "PMID:1084139", "title": "Pathological changes in surgically removed aortocoronary vein grafts.", "content": "Pathological changes in aortocoronary vein grafts were investigated in 17 patients who had a second revascularization procedure 1 to 53 1/2 months after their initial operation. Subendothelial proliferation was present in all grafts and had resulted in total occlusion of 7. With increasing duration of implantation the proliferative lesions tended to show hyalinization and to affect the media. Advanced atherosclerosis had developed in 2 grafts.", "contents": "Pathological changes in surgically removed aortocoronary vein grafts. Pathological changes in aortocoronary vein grafts were investigated in 17 patients who had a second revascularization procedure 1 to 53 1/2 months after their initial operation. Subendothelial proliferation was present in all grafts and had resulted in total occlusion of 7. With increasing duration of implantation the proliferative lesions tended to show hyalinization and to affect the media. Advanced atherosclerosis had developed in 2 grafts."}
{"id": "PMID:1084140", "title": "Unexpected death following aortocoronary bypass.", "content": "Since 1968, 1,800 aortocoronary bypass procedures have been performed with 37 operative or early postoperative deaths (2.1%). Twenty-one deaths (57%) occurred in patients with one or more of the following predetermined risk factors: ventricular aneurysm, decreased left ventricular contractility, left main coronary artery stenosis, valve replacement, recent infarction, or cardiogenic shock. The remaining 16 deaths (43%) were unexpected and occurred in patients with none of these risk factors.", "contents": "Unexpected death following aortocoronary bypass. Since 1968, 1,800 aortocoronary bypass procedures have been performed with 37 operative or early postoperative deaths (2.1%). Twenty-one deaths (57%) occurred in patients with one or more of the following predetermined risk factors: ventricular aneurysm, decreased left ventricular contractility, left main coronary artery stenosis, valve replacement, recent infarction, or cardiogenic shock. The remaining 16 deaths (43%) were unexpected and occurred in patients with none of these risk factors."}
{"id": "PMID:1084141", "title": "Transthoracic intraaortic balloon cannulation to avoid repeat sternotomy for removal.", "content": "Cannulation of the ascending aorta for intraaortic balloon assistance is a practical alternative to the standard transfemoral cannulation in patients with significant aortoiliac occlusive disease. One disadvantage of ascending aortic cannulation has been the requirement for a repeat sternotomy to remove the balloon. This report describes the successful implementation in 2 patients of a technique of ascending aortic balloon insertion and removal that avoids a second sternotomy.", "contents": "Transthoracic intraaortic balloon cannulation to avoid repeat sternotomy for removal. Cannulation of the ascending aorta for intraaortic balloon assistance is a practical alternative to the standard transfemoral cannulation in patients with significant aortoiliac occlusive disease. One disadvantage of ascending aortic cannulation has been the requirement for a repeat sternotomy to remove the balloon. This report describes the successful implementation in 2 patients of a technique of ascending aortic balloon insertion and removal that avoids a second sternotomy."}
{"id": "PMID:1084142", "title": "[Legal abortion and hemostasis. Histological examinations and comparison to post partum hemostasis (author's transl)].", "content": "30 women were examined to determine the hemostasis during the legally induced abortion. The blood coagulated in the intervillous spaces while the fetus is removed. Immediately after the termination of the curettment the nidation area is covered by filaments of fibrin and incorporated erythrocytes. More frequent as post partum platelet thrombi occur especially in the area of decidua parietalis. The intravascular findings are similar to post partum changes after the removal of the placenta. Obstruction clots of the spiral arterioles contain fibrin deposits, pletelet thrombi and erythrocytes (mixed form of thrombi). Residual decidua and trophoblast cells are seldom involved in the obturation of the vessels. The traumatic damages of the uterine wall are described (extravascular fibrin deposits, interstitial bleeding, succulent tissue a.s.o.).", "contents": "[Legal abortion and hemostasis. Histological examinations and comparison to post partum hemostasis (author's transl)]. 30 women were examined to determine the hemostasis during the legally induced abortion. The blood coagulated in the intervillous spaces while the fetus is removed. Immediately after the termination of the curettment the nidation area is covered by filaments of fibrin and incorporated erythrocytes. More frequent as post partum platelet thrombi occur especially in the area of decidua parietalis. The intravascular findings are similar to post partum changes after the removal of the placenta. Obstruction clots of the spiral arterioles contain fibrin deposits, pletelet thrombi and erythrocytes (mixed form of thrombi). Residual decidua and trophoblast cells are seldom involved in the obturation of the vessels. The traumatic damages of the uterine wall are described (extravascular fibrin deposits, interstitial bleeding, succulent tissue a.s.o.)."}
{"id": "PMID:1084143", "title": "The influence of protease inhibitor on hydroproteolytic activity and serotonin content in the intestine of rats after fractionated irradiation.", "content": "Hydroproteolytic activity and content of serotonin in the small intestine and colon were studied in rats X-irradiated on the whole body with fractionated doses of 5 X 150 R. Twenty-four hours after exposure to X-rays, lipase, acid phosphatase activities and cathepsin activity as well as serotonin content were assayed in the intestinal tissues. Irradiation altered the hydroproteolytic activity of the tissues, in a majority of cases by increasing hydrolytic activity and lowering proteolytic (catheptic) activity in all parts of the small intestine and decreased serotonin content in the first and third segments of the small intestine. Trasylol injected s.c. in doses of 10,000 units/kg before and after irradiation modified hydrolytic tissue activity of the studied segments of the intestine and lessened the fall in serotonin content of the intestines after irradiation.", "contents": "The influence of protease inhibitor on hydroproteolytic activity and serotonin content in the intestine of rats after fractionated irradiation. Hydroproteolytic activity and content of serotonin in the small intestine and colon were studied in rats X-irradiated on the whole body with fractionated doses of 5 X 150 R. Twenty-four hours after exposure to X-rays, lipase, acid phosphatase activities and cathepsin activity as well as serotonin content were assayed in the intestinal tissues. Irradiation altered the hydroproteolytic activity of the tissues, in a majority of cases by increasing hydrolytic activity and lowering proteolytic (catheptic) activity in all parts of the small intestine and decreased serotonin content in the first and third segments of the small intestine. Trasylol injected s.c. in doses of 10,000 units/kg before and after irradiation modified hydrolytic tissue activity of the studied segments of the intestine and lessened the fall in serotonin content of the intestines after irradiation."}
{"id": "PMID:1084144", "title": "[Secretory granules in cardiomyocytes].", "content": "The specific granules having the appearance of spherical formations of 2000-4000 A in diameter are described in the cardiocytes of the atria and ventricles of mature frogs (R. temporaria). Each granule consists of electron dense granular matrix and a membrane up to 60 A trick. The amount of these granules in the cardiomyocytes of the atria is greater and they occasionally form accumulations, connected as a rule with the elements of the lamellar complex. The rough reticulum is better developed in the cells containing clusters of granules. The granules described are considered to be secretory ones.", "contents": "[Secretory granules in cardiomyocytes]. The specific granules having the appearance of spherical formations of 2000-4000 A in diameter are described in the cardiocytes of the atria and ventricles of mature frogs (R. temporaria). Each granule consists of electron dense granular matrix and a membrane up to 60 A trick. The amount of these granules in the cardiomyocytes of the atria is greater and they occasionally form accumulations, connected as a rule with the elements of the lamellar complex. The rough reticulum is better developed in the cells containing clusters of granules. The granules described are considered to be secretory ones."}
{"id": "PMID:1084145", "title": "[Morphologic changes in the lymph microcirculation of serous membranes in rheumatism and systemic lupus erythematosus].", "content": "Microcirculation consists of two elements--hemomicrocirculation and lymphomicrocirculation, the both being closely interrelated with each other. Lymphomicrocirculation was studied on the serous membranes taken from dead people who had died of rheumatism and systemic lupus erythematosus (SLE). The microcirculatory bed in the serous membranes was identified by V. V. Kupriyanov's method. The results of the studies showed that in serous membranes in rheumatism there occurred gradual emptying of lymphomicrocirculation and at the same time--compensatory-adaptive and regeneratory processes, the latter being manifested in formation of blind projections of capillaries, in broadening of the lumen. In SLE the lymphatic link actively participated in elimination of metabolism products and underwent a continuous rearrangement with formation of closed and communicant cysterns. Moreover, there was noted a new formation of lymphatic capillaries, which should be considered as a compensatory-adaptive process. Hemo- and lymphomicrocirculation elements are intimately connected due to close contacts between capillaries of the two elements of microcirculation, however, without formation of connecting fistulas. Hemo- and lymphomicrocirculation is an integrate, and under conditions of pathology there developed therein compensatory-adaptive and regenerative processes aimed at the maintenance of tissue metabolic and hemodynamic homeostasis.", "contents": "[Morphologic changes in the lymph microcirculation of serous membranes in rheumatism and systemic lupus erythematosus]. Microcirculation consists of two elements--hemomicrocirculation and lymphomicrocirculation, the both being closely interrelated with each other. Lymphomicrocirculation was studied on the serous membranes taken from dead people who had died of rheumatism and systemic lupus erythematosus (SLE). The microcirculatory bed in the serous membranes was identified by V. V. Kupriyanov's method. The results of the studies showed that in serous membranes in rheumatism there occurred gradual emptying of lymphomicrocirculation and at the same time--compensatory-adaptive and regeneratory processes, the latter being manifested in formation of blind projections of capillaries, in broadening of the lumen. In SLE the lymphatic link actively participated in elimination of metabolism products and underwent a continuous rearrangement with formation of closed and communicant cysterns. Moreover, there was noted a new formation of lymphatic capillaries, which should be considered as a compensatory-adaptive process. Hemo- and lymphomicrocirculation elements are intimately connected due to close contacts between capillaries of the two elements of microcirculation, however, without formation of connecting fistulas. Hemo- and lymphomicrocirculation is an integrate, and under conditions of pathology there developed therein compensatory-adaptive and regenerative processes aimed at the maintenance of tissue metabolic and hemodynamic homeostasis."}
{"id": "PMID:1084146", "title": "[Lymphoid tissue and the body's non-specific resistance].", "content": "In experiments on rats and mice the author and his pupils showed that nonspecific effect of stimulators brought about in the first hours following the exposure migration of cells from the lymphoid organs, increase in the number of lymphoid cells in the bone marrow by 40-60%, appearance in the bone marrow of T-cells, and increase in the number of hemopoietic cells (CFU). This contributed to the activation of hemopoiesis and to development of transient hyperplasia of the bone marrow to the stage of elevated resistance of the organism. It was shown that transplantation of T-cells performed in the first hours after the sublethal irradiation hastened regeneration of hemopoiesis. All the data indicated above testify to the role of the lymphoid tissue in nonspecific resistance of the organism.", "contents": "[Lymphoid tissue and the body's non-specific resistance]. In experiments on rats and mice the author and his pupils showed that nonspecific effect of stimulators brought about in the first hours following the exposure migration of cells from the lymphoid organs, increase in the number of lymphoid cells in the bone marrow by 40-60%, appearance in the bone marrow of T-cells, and increase in the number of hemopoietic cells (CFU). This contributed to the activation of hemopoiesis and to development of transient hyperplasia of the bone marrow to the stage of elevated resistance of the organism. It was shown that transplantation of T-cells performed in the first hours after the sublethal irradiation hastened regeneration of hemopoiesis. All the data indicated above testify to the role of the lymphoid tissue in nonspecific resistance of the organism."}
{"id": "PMID:1084147", "title": "[Meningo-encephalic tuberculosis in childhood. Anatomo pathological study of 10 cases].", "content": "The anatomo-pathological study of ten cases of tuberculosis of the central nervous system (CNS) in children under 10 years old is reported. The emphasis is given to the macroscopic and microscopic changes produced by the tuberculosis in the meninges, in the nervous parenchyma  and in the vessels. The clinical and pathological findings are discussed and correlated with the literature. The authors concluded that: 1) the lesion in children with tuberculosis of the CNS is generally a proliferative meningoencephalitis; 2) the nervous parenchyma may be affected by the contiguous propagation of the tuberculous process or by the circulatory changes secondary to the arterial lesions; 3) because of the localization of the endocranial vessels in the sub-arachnoidal space they are generally injured by the inflammatory process, which can determine an obliterating endarteritis, which can be observed by a carotid angiography.", "contents": "[Meningo-encephalic tuberculosis in childhood. Anatomo pathological study of 10 cases]. The anatomo-pathological study of ten cases of tuberculosis of the central nervous system (CNS) in children under 10 years old is reported. The emphasis is given to the macroscopic and microscopic changes produced by the tuberculosis in the meninges, in the nervous parenchyma  and in the vessels. The clinical and pathological findings are discussed and correlated with the literature. The authors concluded that: 1) the lesion in children with tuberculosis of the CNS is generally a proliferative meningoencephalitis; 2) the nervous parenchyma may be affected by the contiguous propagation of the tuberculous process or by the circulatory changes secondary to the arterial lesions; 3) because of the localization of the endocranial vessels in the sub-arachnoidal space they are generally injured by the inflammatory process, which can determine an obliterating endarteritis, which can be observed by a carotid angiography."}
{"id": "PMID:1084148", "title": "The transcochlear approach to the skull base.", "content": "A new approach to tumors of the skull base is described. This approach is accomplished by forward extension of the translabyrinthine opening into the cerebellopontine angle. The facial nerve is mobilized in the temporal bone from the stylomastoid foramen to its entrance into the internal auditory canal. Having removed the barrier of the facial nerve, additional bone removal can be carried forward to the internal carotid artery, which now becomes the forward limit for temporal bone resection. The access attained through this exposure allows removal of tumors arising from the petrous tip, as well as tumors arising directly from the clivus. We provide the case histories of four patients in whom this approach was successfully used in removal of skull base tumors.", "contents": "The transcochlear approach to the skull base. A new approach to tumors of the skull base is described. This approach is accomplished by forward extension of the translabyrinthine opening into the cerebellopontine angle. The facial nerve is mobilized in the temporal bone from the stylomastoid foramen to its entrance into the internal auditory canal. Having removed the barrier of the facial nerve, additional bone removal can be carried forward to the internal carotid artery, which now becomes the forward limit for temporal bone resection. The access attained through this exposure allows removal of tumors arising from the petrous tip, as well as tumors arising directly from the clivus. We provide the case histories of four patients in whom this approach was successfully used in removal of skull base tumors."}
{"id": "PMID:1084149", "title": "Alpha 1-antitrypsin activity of perilymph. Occurrence during progression of otospongiosis.", "content": "Previous studies by the Adams method demonstrated a strong correlation between hydrolytic enzyme activity of perilymph and progression of bone conduction loss two years preceding stapedectomy. Alpha 1-Antitrypsin was chosen since its activity can be very precisely measured by a radical immunodiffusion technique and since it is one of the enzymes identified in perilymph of patients with active otospongiosis. Samples of 3 mul to 5 mul of perilymph removed during 103 stapedectomies and samples of known alpha 1-trypsin activity were placed on slides coated with alpha 1-antitrypsin serum. The zone of diffusion was stained and measured after 38 hours of incubation. Antitrypsin values were lowest in 24 cases, two with no preoperative bone conduction progression, three with moderate progression of 10 to 15 dB, and 19 with rapid progression of more than 20 dB. They were highest in 36 cases with no progression, and in one case with moderate progression. This study confirms previous reports on the enzymatic activity in otospongiotic disease.", "contents": "Alpha 1-antitrypsin activity of perilymph. Occurrence during progression of otospongiosis. Previous studies by the Adams method demonstrated a strong correlation between hydrolytic enzyme activity of perilymph and progression of bone conduction loss two years preceding stapedectomy. Alpha 1-Antitrypsin was chosen since its activity can be very precisely measured by a radical immunodiffusion technique and since it is one of the enzymes identified in perilymph of patients with active otospongiosis. Samples of 3 mul to 5 mul of perilymph removed during 103 stapedectomies and samples of known alpha 1-trypsin activity were placed on slides coated with alpha 1-antitrypsin serum. The zone of diffusion was stained and measured after 38 hours of incubation. Antitrypsin values were lowest in 24 cases, two with no preoperative bone conduction progression, three with moderate progression of 10 to 15 dB, and 19 with rapid progression of more than 20 dB. They were highest in 36 cases with no progression, and in one case with moderate progression. This study confirms previous reports on the enzymatic activity in otospongiotic disease."}
{"id": "PMID:1084151", "title": "alpha-1-Anti-trypsin, an inhibitor of renin.", "content": "A naturally occurring competitive inhibitor of pig kidney renin has been identified in human plasma. The inhibitor was shown to be alpha-1 anti-trypsin and the effect in vitro on the renin activity was examined. The slope in the Hill plot is compatible with the assumption of one-site competitive inhibition. Other proteinase inhibitors, such as alpha-2-macroglobulin and C1 inactivator, however, have no inhibitory effect on the renin-angiotensinogen reaction.", "contents": "alpha-1-Anti-trypsin, an inhibitor of renin. A naturally occurring competitive inhibitor of pig kidney renin has been identified in human plasma. The inhibitor was shown to be alpha-1 anti-trypsin and the effect in vitro on the renin activity was examined. The slope in the Hill plot is compatible with the assumption of one-site competitive inhibition. Other proteinase inhibitors, such as alpha-2-macroglobulin and C1 inactivator, however, have no inhibitory effect on the renin-angiotensinogen reaction."}
{"id": "PMID:1084150", "title": "Cochleo-vestibular disturbances in diving.", "content": "Insidious development of high-tone sensorineural hearing loss may be associated with diving, but the evidence is not certain and further research is needed. 'Internal ear barotrauma' can cause an acute or relatively acute onset of hearing loss and/or vertigo, and it may be that 'alternobaric vertigo' provides a link between the insidious and acute forms of labyrinthine injury in diving. With deep diving, decompression sickness and other syndromes can also affect the cochleo-vestibular system. These aetiologies and effects will be discussed, together with evidence from an audiometric survey of naval divers and of 5 experimental deep divers.", "contents": "Cochleo-vestibular disturbances in diving. Insidious development of high-tone sensorineural hearing loss may be associated with diving, but the evidence is not certain and further research is needed. 'Internal ear barotrauma' can cause an acute or relatively acute onset of hearing loss and/or vertigo, and it may be that 'alternobaric vertigo' provides a link between the insidious and acute forms of labyrinthine injury in diving. With deep diving, decompression sickness and other syndromes can also affect the cochleo-vestibular system. These aetiologies and effects will be discussed, together with evidence from an audiometric survey of naval divers and of 5 experimental deep divers."}
{"id": "PMID:1084153", "title": "Effect of aprotinin on immunological resistance in tumour-bearing animals.", "content": "Previous studies suggested that aprotinin might enhance the host's immunological resistance to tumours. This possibility has now been further investigated by studying the behaviour of tumours in both hamsters and mice. A second tumour graft in tumour-bearing hamsters appeared more rapidly than the first. Prior administration of aprotinin abolished this effect. Pretreatment of non-cancerous mice with cortisone nullified the effectiveness of aprotinin in inhibiting the growth of a subsequent tumour graft. These results are interpreted as additional evidence that aprotinin enhances the immunological system against tumour cells.", "contents": "Effect of aprotinin on immunological resistance in tumour-bearing animals. Previous studies suggested that aprotinin might enhance the host's immunological resistance to tumours. This possibility has now been further investigated by studying the behaviour of tumours in both hamsters and mice. A second tumour graft in tumour-bearing hamsters appeared more rapidly than the first. Prior administration of aprotinin abolished this effect. Pretreatment of non-cancerous mice with cortisone nullified the effectiveness of aprotinin in inhibiting the growth of a subsequent tumour graft. These results are interpreted as additional evidence that aprotinin enhances the immunological system against tumour cells."}
{"id": "PMID:1084157", "title": "Immunological classification of acute lymphoblastic leukaemias: evaluation of its clinical significance in a hundred patients.", "content": "The use of T and B lymphocyte markers and of different antisera raised against malignant B cells and fetal thymocytes allowed the classification of 100 patients with acute lymphoblastic leukemia (ALL) into three groups. (I) Patients with non-T non-B ALL whose cells were devoid of conventional B and T markers but characterized by a leukaemia associated antigen (69 cases). (2) Patients with T-derived ALL (28 cases). (3) Patients with ALL of B cell origin (three cases). The search for haematological and clinical correlations showed that those patients with T-derived ALL tended to have a higher leucocyte count (P=0.05) and acid phosphatase positivity of blast cells (P= 0.01), a higher incidence of tumour presentation (P=0.05) and a thymic mass. Survival curves for the two main groups of patients are similar at 36 months but meningeal relapses were more frequent in patients with T-derived ALL (P=0.02).", "contents": "Immunological classification of acute lymphoblastic leukaemias: evaluation of its clinical significance in a hundred patients. The use of T and B lymphocyte markers and of different antisera raised against malignant B cells and fetal thymocytes allowed the classification of 100 patients with acute lymphoblastic leukemia (ALL) into three groups. (I) Patients with non-T non-B ALL whose cells were devoid of conventional B and T markers but characterized by a leukaemia associated antigen (69 cases). (2) Patients with T-derived ALL (28 cases). (3) Patients with ALL of B cell origin (three cases). The search for haematological and clinical correlations showed that those patients with T-derived ALL tended to have a higher leucocyte count (P=0.05) and acid phosphatase positivity of blast cells (P= 0.01), a higher incidence of tumour presentation (P=0.05) and a thymic mass. Survival curves for the two main groups of patients are similar at 36 months but meningeal relapses were more frequent in patients with T-derived ALL (P=0.02)."}
{"id": "PMID:1084158", "title": "Quantitative determination of factor-VIII protein by two-stage gel electrophoresis.", "content": "A new method for the quantification of factor-VIII protein in plasma is described which utilizes only commercially available equipment and material. The procedure involves separation of factor VII from other plasma proteins by a two-stage sodium dodecyl sulphate gel electrophoretic technique and quantification of the protein by scanning densitometry. The plasma from normal individuals, haemophiliacs, obligate carriers of haemophilia and patients with von Willebrand's disease have been examined by this technique. In general, the data support the earlier findings obtained by quantitative immunoelectrophoresis. In addition, some abnormalities not detected by immunoelectrophoresis are observed by the gel electrophoresis technique.", "contents": "Quantitative determination of factor-VIII protein by two-stage gel electrophoresis. A new method for the quantification of factor-VIII protein in plasma is described which utilizes only commercially available equipment and material. The procedure involves separation of factor VII from other plasma proteins by a two-stage sodium dodecyl sulphate gel electrophoretic technique and quantification of the protein by scanning densitometry. The plasma from normal individuals, haemophiliacs, obligate carriers of haemophilia and patients with von Willebrand's disease have been examined by this technique. In general, the data support the earlier findings obtained by quantitative immunoelectrophoresis. In addition, some abnormalities not detected by immunoelectrophoresis are observed by the gel electrophoresis technique."}
{"id": "PMID:1084159", "title": "Droplet degeneration of the cornea in North Cameroon. Prevalence and clinical appearances.", "content": "The prevalence of droplet degeneration of the cornea in a defined population of 517 persons in north Cameroon was similar in males and females, but severe changes were commoner in elderly men. Most cases showed only mild changes of granularity and droplet formation in Bowman's zone, but they were sometimes widespread. More severe changes were seen in only nine patients. The clinical appearances are compared with those described from other parts of the world. In support of the aetiological role of direct sunlight, droplet degeneration was much more prevalent in the savanna than in the rain-forest in Cameroon. The condition ranges in severity from very mild to very severe, but difficulties in differential diagnosis, particularly in advanced cases, make it doubtful whether the same basic condition is being described in all cases. These doubts will be resolved only by properly conducted epidemiological studies in different parts of the world.", "contents": "Droplet degeneration of the cornea in North Cameroon. Prevalence and clinical appearances. The prevalence of droplet degeneration of the cornea in a defined population of 517 persons in north Cameroon was similar in males and females, but severe changes were commoner in elderly men. Most cases showed only mild changes of granularity and droplet formation in Bowman's zone, but they were sometimes widespread. More severe changes were seen in only nine patients. The clinical appearances are compared with those described from other parts of the world. In support of the aetiological role of direct sunlight, droplet degeneration was much more prevalent in the savanna than in the rain-forest in Cameroon. The condition ranges in severity from very mild to very severe, but difficulties in differential diagnosis, particularly in advanced cases, make it doubtful whether the same basic condition is being described in all cases. These doubts will be resolved only by properly conducted epidemiological studies in different parts of the world."}
{"id": "PMID:1084160", "title": "A 4-vinylprotochlorophyllide complex accumulated by \"phofil\" mutant of Rhodopseudomonas spheroides. An authentic intermediate in the development of the photosynthetic apparatus.", "content": "A photosynthetically competent mutant strain of Rhodopseudomonas spheroides was isolated. In addition to bacteriochlorophyll, this organism produced particle-bound precursor 4-vinylprotochlorophyllide. The spectral characteristics of the pigment complexes(es) accumulated in the culture medium were very variable. The spectral form occurring within the bacteria was characterized from fluorescence data. Its particle weight, 130 000, was determined by Sephadex G200 filtration. The main components of the complex were protein, lipid and pigment (6.8:61, w/w). As indicated by qualitative analysis, the lipid components were characteristic constituents of the photosynthetic membrane. Kinetics of pigments synthesis showed that the total pigment synthesis was not affected by the mutation; bacteriochlorophyll content was always lower in the mutant than in the parent strain. The repigmentation process was followed by fluorescence emission. The results indicated that the mutation affected membrane component synthesis required for the bacteriochlorophyll(ide) incorporation. The pigment complex was concluded to be an authentic intermediate in photosynthetic apparatus morphogenesis. The reasons for its excretion are discussed.", "contents": "A 4-vinylprotochlorophyllide complex accumulated by \"phofil\" mutant of Rhodopseudomonas spheroides. An authentic intermediate in the development of the photosynthetic apparatus. A photosynthetically competent mutant strain of Rhodopseudomonas spheroides was isolated. In addition to bacteriochlorophyll, this organism produced particle-bound precursor 4-vinylprotochlorophyllide. The spectral characteristics of the pigment complexes(es) accumulated in the culture medium were very variable. The spectral form occurring within the bacteria was characterized from fluorescence data. Its particle weight, 130 000, was determined by Sephadex G200 filtration. The main components of the complex were protein, lipid and pigment (6.8:61, w/w). As indicated by qualitative analysis, the lipid components were characteristic constituents of the photosynthetic membrane. Kinetics of pigments synthesis showed that the total pigment synthesis was not affected by the mutation; bacteriochlorophyll content was always lower in the mutant than in the parent strain. The repigmentation process was followed by fluorescence emission. The results indicated that the mutation affected membrane component synthesis required for the bacteriochlorophyll(ide) incorporation. The pigment complex was concluded to be an authentic intermediate in photosynthetic apparatus morphogenesis. The reasons for its excretion are discussed."}
{"id": "PMID:1084162", "title": "Separation of human lymphoid cells by preparative cell electrophoresis. III. Studies on cells of lymphoid organs in hematological normal persons.", "content": "Blood and bone marrow lymphocytes and cells of lymphoid organs in hematologically normal persons have been studied using free-flow electrophoresis by which a preparative separation of cells according to their electrophoretic mobility is achieved. The results are demonstrated as electrophoretic distributions. Blood lymphocytes have a peak corresponding to relative high electrophoretic mobility, whereas the majority of marrow lymphocytes is found in fractions corresponding to low electrophoretic mobility. The distributions of small lymphocytes from tonsil, spleen and lymphnode either resemble the distribution of blood lymphocytes or have a bimodal fashion with peaks corresponding to blood lymphocytes or marrow lymphocytes. Large lymphoid cells capable of DNA synthesis are concentrated in fractions of intermediate mobility. The results confirm the concept that peripheral T cells have a high electrophoretic mobility whereas most B cells are of low mobility. In contrast to peripheral T cells, cells of the thymus have an intermediate or low electrophoretic mobility. Thus, it appears that in the lymphoid cell system differences in the electrophoretic mobility not only reflect different functional properties but also differences in maturation stages.", "contents": "Separation of human lymphoid cells by preparative cell electrophoresis. III. Studies on cells of lymphoid organs in hematological normal persons. Blood and bone marrow lymphocytes and cells of lymphoid organs in hematologically normal persons have been studied using free-flow electrophoresis by which a preparative separation of cells according to their electrophoretic mobility is achieved. The results are demonstrated as electrophoretic distributions. Blood lymphocytes have a peak corresponding to relative high electrophoretic mobility, whereas the majority of marrow lymphocytes is found in fractions corresponding to low electrophoretic mobility. The distributions of small lymphocytes from tonsil, spleen and lymphnode either resemble the distribution of blood lymphocytes or have a bimodal fashion with peaks corresponding to blood lymphocytes or marrow lymphocytes. Large lymphoid cells capable of DNA synthesis are concentrated in fractions of intermediate mobility. The results confirm the concept that peripheral T cells have a high electrophoretic mobility whereas most B cells are of low mobility. In contrast to peripheral T cells, cells of the thymus have an intermediate or low electrophoretic mobility. Thus, it appears that in the lymphoid cell system differences in the electrophoretic mobility not only reflect different functional properties but also differences in maturation stages."}
{"id": "PMID:1084163", "title": "Local anesthetic alteration of miniature endplate currents and endplate current fluctuations.", "content": "The effect of the local anesthetic QX222 on the kinetics of miniature endplate currents and acetylcholine induced endplate current fluctuations was studied in voltage clamped cutaneous pectoris muscle of Rana pipiens. Both the endplate current fluctuation spectra and the miniature endplate current decay consisted of two or three components depending upon the holding potential and local anesthetic concentration. The cutoff frequency of each spectral component was equal to the decay rate of its corresponding constituent of the miniature endplate current. Comparison of the relative amplitudes of the spectral and miniature endplate components indicated that QX222 did not act by creating two kinetically distinct populations of acetylcholine receptors. QX222 action could be explained by alteration of the acetylcholine receptors such that they sequentially change conformation form one open state to another. A specific case in which QX222 binds to the open state of the acetyl-choline receptor creating a blocked state, was found to account for the observed relationship between the relative amplitudes of the miniature endplate current and spectral components, as well as the previously observed voltage and concentration sensitivity of the decay rates of endplate current components.", "contents": "Local anesthetic alteration of miniature endplate currents and endplate current fluctuations. The effect of the local anesthetic QX222 on the kinetics of miniature endplate currents and acetylcholine induced endplate current fluctuations was studied in voltage clamped cutaneous pectoris muscle of Rana pipiens. Both the endplate current fluctuation spectra and the miniature endplate current decay consisted of two or three components depending upon the holding potential and local anesthetic concentration. The cutoff frequency of each spectral component was equal to the decay rate of its corresponding constituent of the miniature endplate current. Comparison of the relative amplitudes of the spectral and miniature endplate components indicated that QX222 did not act by creating two kinetically distinct populations of acetylcholine receptors. QX222 action could be explained by alteration of the acetylcholine receptors such that they sequentially change conformation form one open state to another. A specific case in which QX222 binds to the open state of the acetyl-choline receptor creating a blocked state, was found to account for the observed relationship between the relative amplitudes of the miniature endplate current and spectral components, as well as the previously observed voltage and concentration sensitivity of the decay rates of endplate current components."}
{"id": "PMID:1084164", "title": "Myelin x-ray patterns reconciled.", "content": "Two different X-ray diffraction patterns have been published for frog sciatic myelin. Here the apparent discrepancy is attributed to different spacings between the myelin membranes in the two experiments. Assuming the single membrane has the same structure in the two cases, some restrictions on the phasing are indicated. Several possible profiles for the single membrane are then considered. A profile derived by assuming a lecithin cholesterol-like bilayer within the membrane accounts for all the published data. Three published profiles also are considered. These are not quite in as good agreement with observation, but they cannot be excluded at present.", "contents": "Myelin x-ray patterns reconciled. Two different X-ray diffraction patterns have been published for frog sciatic myelin. Here the apparent discrepancy is attributed to different spacings between the myelin membranes in the two experiments. Assuming the single membrane has the same structure in the two cases, some restrictions on the phasing are indicated. Several possible profiles for the single membrane are then considered. A profile derived by assuming a lecithin cholesterol-like bilayer within the membrane accounts for all the published data. Three published profiles also are considered. These are not quite in as good agreement with observation, but they cannot be excluded at present."}
{"id": "PMID:1084165", "title": "Pulsed nuclear magnetic resonance study of 17O from H217O in rat lymphocytes.", "content": "Lymphocytes obtained from thymus glands of normal rats and culture lines of malignant rat thymocytes were enriched with H217O. The longitudinal and transverse relaxations of the 17O were determined separately in samples of packed cells and supernatant solutions. The longitudinal relaxation of intracellular 17O of fresh viable lymphocytes was nonexponential, becoming simply exponential with eventual necrosis. The rate of spin-lattice relaxation (1/T1) was fitted by a sum of two exponentials. The average mole fraction of the molecules subject to the slower relaxation rate (1/T1s) was two-thirds of the total water. Lowering the Larmor frequency (omega) from 7.72 to 4.36 MHZ increased the faster component (1/T1f) by 12% without altering (1/T1s). The value of the single exponential decay of the nonviable cells was not appreciably different from the initial rate of relaxation of the fresh cells. Similar results were obtained in studies of the transverse relaxation rates. The simplest interpretation is that two-thirds of the cell water is located within the nucelus and is characterized by a slower rate of relaxation than the one-third of the cell water in the cytoplasm because of the different macromolecular compositions of the two-subcellular compartments. The malignant lymphocytes were characterized by prolonged values for the slow and fast components of both the longitudinal and transverse relaxations of 17O.", "contents": "Pulsed nuclear magnetic resonance study of 17O from H217O in rat lymphocytes. Lymphocytes obtained from thymus glands of normal rats and culture lines of malignant rat thymocytes were enriched with H217O. The longitudinal and transverse relaxations of the 17O were determined separately in samples of packed cells and supernatant solutions. The longitudinal relaxation of intracellular 17O of fresh viable lymphocytes was nonexponential, becoming simply exponential with eventual necrosis. The rate of spin-lattice relaxation (1/T1) was fitted by a sum of two exponentials. The average mole fraction of the molecules subject to the slower relaxation rate (1/T1s) was two-thirds of the total water. Lowering the Larmor frequency (omega) from 7.72 to 4.36 MHZ increased the faster component (1/T1f) by 12% without altering (1/T1s). The value of the single exponential decay of the nonviable cells was not appreciably different from the initial rate of relaxation of the fresh cells. Similar results were obtained in studies of the transverse relaxation rates. The simplest interpretation is that two-thirds of the cell water is located within the nucelus and is characterized by a slower rate of relaxation than the one-third of the cell water in the cytoplasm because of the different macromolecular compositions of the two-subcellular compartments. The malignant lymphocytes were characterized by prolonged values for the slow and fast components of both the longitudinal and transverse relaxations of 17O."}
{"id": "PMID:1084166", "title": "[The role of catecholamines in the centrifugal regulation of taste receptors].", "content": "An electrophysiological method was used to study the centrifugal inhibition of the impulse activity of taste receptors of the frog tongue. A deficiency of catecholamines in the organism (reserpinization) or perfusion of the adrenaline-blocking agents through the lingual vessel (propranololi hydrochloridum, dibenamine) were found to prevent the centrifugal inhibition, while perfusion with an adrenomimetic agent (epinephrine) enhanced this effect. Such effects of the adrenergic agents were also observed in the animals which were subjected to desympathization by bilateral extirpation of the first ganglion of the sympathetic chain. A conclusion was drawn that catecholamines in the papilli were necessary to obtain the central inhibition influences on the function of the taste receptor apparatus.", "contents": "[The role of catecholamines in the centrifugal regulation of taste receptors]. An electrophysiological method was used to study the centrifugal inhibition of the impulse activity of taste receptors of the frog tongue. A deficiency of catecholamines in the organism (reserpinization) or perfusion of the adrenaline-blocking agents through the lingual vessel (propranololi hydrochloridum, dibenamine) were found to prevent the centrifugal inhibition, while perfusion with an adrenomimetic agent (epinephrine) enhanced this effect. Such effects of the adrenergic agents were also observed in the animals which were subjected to desympathization by bilateral extirpation of the first ganglion of the sympathetic chain. A conclusion was drawn that catecholamines in the papilli were necessary to obtain the central inhibition influences on the function of the taste receptor apparatus."}
{"id": "PMID:1084167", "title": "[The effect of the polyvalent proteinase inhibitor, trasylol, on antibody formation under normal conditions and in experimental atherosclerosis].", "content": "Antibodies to erythrocytes of sheep and human serum albumin were formed in rabbits with alimentary atherosclerosis as intensively as in control. Trasilol failed to affect the antibody formation in normal animals, but sharply decreased the production of specific immunoglobulins (especially the 7S) in rabbits with experimental atherosclerosis. Thus, the action of the preparation is realized not by way of direct inhibition of functional activity of the lymphoid tissue cells, but by inhibiting the immunostimulating factor circulating in the blood in experimental atherosclerosis.", "contents": "[The effect of the polyvalent proteinase inhibitor, trasylol, on antibody formation under normal conditions and in experimental atherosclerosis]. Antibodies to erythrocytes of sheep and human serum albumin were formed in rabbits with alimentary atherosclerosis as intensively as in control. Trasilol failed to affect the antibody formation in normal animals, but sharply decreased the production of specific immunoglobulins (especially the 7S) in rabbits with experimental atherosclerosis. Thus, the action of the preparation is realized not by way of direct inhibition of functional activity of the lymphoid tissue cells, but by inhibiting the immunostimulating factor circulating in the blood in experimental atherosclerosis."}
{"id": "PMID:1084168", "title": "[Electrotonic potentials of spinal cord dorsal roots in frogs with experimental tetanus].", "content": "Tetanus toxin administered to frogs in doses which produced general tetanus depressed the reciprocal inhibition of spinal reflexes. This depression was not associated with the block of the processes which led to depolarization fo the central terminals of the primary afferent fibers.", "contents": "[Electrotonic potentials of spinal cord dorsal roots in frogs with experimental tetanus]. Tetanus toxin administered to frogs in doses which produced general tetanus depressed the reciprocal inhibition of spinal reflexes. This depression was not associated with the block of the processes which led to depolarization fo the central terminals of the primary afferent fibers."}
{"id": "PMID:1084169", "title": "[Quantitative characteristics of the phenomenon of acceleration of hematopoiesis regeneration in thymocyte transplantation].", "content": "Experiments were conducted on hybrid mice (CBA X C57BL) F1. The quantitative characteristics of the phenomenon of acceleration of postirradiation bone marrow regeneration in transplantation of syngenous thymocytes was given. It was revealed that in the range of 10(6)--10(8) cells the effect was independent of the number of transplanted thymocytes. The transplantation during the first 12 hours after the irradiation was equally effective from the aspect of stimulation of the bone marrow restoration. This phenomenon was not expressed under the following conditions: a) transplantation of thymocytes 24 hours after irradiation; b) irradiation of recipients in a dose of 600--700 R; c) irradiation of a thymocyte suspension in vitro in a dose of 10 kR with a subsequent incubation at 37 degrees C for 6--7 hours; d) irradiation of thymocytes in vitro in a dose of 200 kR.", "contents": "[Quantitative characteristics of the phenomenon of acceleration of hematopoiesis regeneration in thymocyte transplantation]. Experiments were conducted on hybrid mice (CBA X C57BL) F1. The quantitative characteristics of the phenomenon of acceleration of postirradiation bone marrow regeneration in transplantation of syngenous thymocytes was given. It was revealed that in the range of 10(6)--10(8) cells the effect was independent of the number of transplanted thymocytes. The transplantation during the first 12 hours after the irradiation was equally effective from the aspect of stimulation of the bone marrow restoration. This phenomenon was not expressed under the following conditions: a) transplantation of thymocytes 24 hours after irradiation; b) irradiation of recipients in a dose of 600--700 R; c) irradiation of a thymocyte suspension in vitro in a dose of 10 kR with a subsequent incubation at 37 degrees C for 6--7 hours; d) irradiation of thymocytes in vitro in a dose of 200 kR."}
{"id": "PMID:1084170", "title": "[The influence of 1-DOPA on emotional responses and serotonin metabolism in the rat brain].", "content": "Experiments were conducted on male Wistar rats. Intraperitoneal injection of 1-DOPA (.100 - 200 mg/kg increased the brain concentration of dopamine and homovanilinic acid and lowered the level of brain serotonin, with simultaneous elevation of its metabolite 5-HIAA. A decrease in serotonin level was accompanied by increased emotional reactivity and agressiveness in rats. L-DOPA (100 mg/kg) decreased the binding of serotonin formed from tryptophane (100 mg/kg), accelerating its catabolism in the brain; at the same time 1-DOPA eliminated the depressive action of tryptophane on the emotional reactivity and aggressiveness. It is supposed that increased emotional excitation elicited by 1-DOPA was partially mediated through the block of the serotoninergic system.", "contents": "[The influence of 1-DOPA on emotional responses and serotonin metabolism in the rat brain]. Experiments were conducted on male Wistar rats. Intraperitoneal injection of 1-DOPA (.100 - 200 mg/kg increased the brain concentration of dopamine and homovanilinic acid and lowered the level of brain serotonin, with simultaneous elevation of its metabolite 5-HIAA. A decrease in serotonin level was accompanied by increased emotional reactivity and agressiveness in rats. L-DOPA (100 mg/kg) decreased the binding of serotonin formed from tryptophane (100 mg/kg), accelerating its catabolism in the brain; at the same time 1-DOPA eliminated the depressive action of tryptophane on the emotional reactivity and aggressiveness. It is supposed that increased emotional excitation elicited by 1-DOPA was partially mediated through the block of the serotoninergic system."}
{"id": "PMID:1084171", "title": "[Dilatation of arterioles in the frog submaxillary muscle under the influence of visible light].", "content": "Frog denervated submaxillar muscle arterioles dilates under the influence of visible light. In darkness the vessel returns to tis initial condition. The degree of dilatation is in the main inversely proportional to the initial diameter of the vessel. Besides, the vessel reactivity also depends on the thickness of its wall. Thick-walled arterioles are the most reactive ones. Their diameter becomes 2 -3 times greater under the influence of visible light. This light-evoked vasodilatation should be taken into account during intravital microscopy, as such a considerable dilatation of arterioles caused by light changes their reactivity considerably.", "contents": "[Dilatation of arterioles in the frog submaxillary muscle under the influence of visible light]. Frog denervated submaxillar muscle arterioles dilates under the influence of visible light. In darkness the vessel returns to tis initial condition. The degree of dilatation is in the main inversely proportional to the initial diameter of the vessel. Besides, the vessel reactivity also depends on the thickness of its wall. Thick-walled arterioles are the most reactive ones. Their diameter becomes 2 -3 times greater under the influence of visible light. This light-evoked vasodilatation should be taken into account during intravital microscopy, as such a considerable dilatation of arterioles caused by light changes their reactivity considerably."}
{"id": "PMID:1084172", "title": "[The effect of tryptophan, 5-hydroxytryptophan, serotonin, histidine and histamine on the peroxidation of lipids in hepatic mitochondrial membranes].", "content": "The method of recording the superweak chemifluorescence was applied to the study of the effect of tryptophan, 5-oxytryptophan, sertonin, histidine and histamine on the peroxication of lipids in the membranes of mitochondria of rat liver in the presence of Fe++ ions inducing this process. 5-oxytryptophan and serotonin inhibited this process in a concentration of 10(-5)-10(-4) M (protein content -1.8 mg per 1 ml of mitochondrial suspension). On the basis of studying the kinetics of the initial portion of the ascending branch of the \"slow flare up\" constants of the antioxidatinve activity (constituting 2.2-10(3) and 9.8-10(3) M-1 for 5-oxytryptophan and serotonin, respectively) was calculated. The antioxidative action is associated with the presence of the phenol group in the molecule of the compound under study. It is supposed that the action of 5-oxytryptophan and serotonin on peroxidation of lipids in the membranes was of significance for the regulation of permeability of the biological membranes, along with their effect on the other membrane processes.", "contents": "[The effect of tryptophan, 5-hydroxytryptophan, serotonin, histidine and histamine on the peroxidation of lipids in hepatic mitochondrial membranes]. The method of recording the superweak chemifluorescence was applied to the study of the effect of tryptophan, 5-oxytryptophan, sertonin, histidine and histamine on the peroxication of lipids in the membranes of mitochondria of rat liver in the presence of Fe++ ions inducing this process. 5-oxytryptophan and serotonin inhibited this process in a concentration of 10(-5)-10(-4) M (protein content -1.8 mg per 1 ml of mitochondrial suspension). On the basis of studying the kinetics of the initial portion of the ascending branch of the \"slow flare up\" constants of the antioxidatinve activity (constituting 2.2-10(3) and 9.8-10(3) M-1 for 5-oxytryptophan and serotonin, respectively) was calculated. The antioxidative action is associated with the presence of the phenol group in the molecule of the compound under study. It is supposed that the action of 5-oxytryptophan and serotonin on peroxidation of lipids in the membranes was of significance for the regulation of permeability of the biological membranes, along with their effect on the other membrane processes."}
{"id": "PMID:1084173", "title": "[Assessment of the quantity of T-lymphocytes and other rosette-forming cells in the blood of patients and healthy subjects].", "content": "In using the reaction of spontaneous rosette formation to assess the immunity T-system one should not limit one-self to determination of the percentage content of the rosette-forming cells alone. Immune status can be properly judged by determination of the content of immunocompetent cells per one cubic mm of the blood of the given donor. When the reaction is staged on the leukocyte suspension not only lymphocytes, but also other cell forms with the capacity of binding sheep erythrocytes can be determined.", "contents": "[Assessment of the quantity of T-lymphocytes and other rosette-forming cells in the blood of patients and healthy subjects]. In using the reaction of spontaneous rosette formation to assess the immunity T-system one should not limit one-self to determination of the percentage content of the rosette-forming cells alone. Immune status can be properly judged by determination of the content of immunocompetent cells per one cubic mm of the blood of the given donor. When the reaction is staged on the leukocyte suspension not only lymphocytes, but also other cell forms with the capacity of binding sheep erythrocytes can be determined."}
{"id": "PMID:1084174", "title": "[The effect of 5-hydroxytryptophan on shivering thermogenesis].", "content": "Studies carried out on ground squirrels showed active warming of these animals during arousal from  hibernation to be closely connected with intensification of the shivering thermogenesis. The intraabdominal administration of 5-oxytryptophane, a precursor of serotonin clearly suppressed the shivering thermogenesis in arising ground squirrels and slowed down their warming.", "contents": "[The effect of 5-hydroxytryptophan on shivering thermogenesis]. Studies carried out on ground squirrels showed active warming of these animals during arousal from  hibernation to be closely connected with intensification of the shivering thermogenesis. The intraabdominal administration of 5-oxytryptophane, a precursor of serotonin clearly suppressed the shivering thermogenesis in arising ground squirrels and slowed down their warming."}
{"id": "PMID:1084175", "title": "[Immunologic competence of T- and B-lymphocytes in tolerance induced by cyclophosphamide].", "content": "A study was made of immunological competence of T- and B-lymphocytes of mice subjected to tolerogenic treatment (administration of a massive dose of sheep erythrocytes and cyclophosphamide 7 days before the experiment). The capacity of lymphocytes of tolerant mice to influence the interaction of normal T- and B-lymphocytes was also investigated. This form of tolerance was caused not by T-suppressors, but by a true deficiency of T-cells-helpers (both in the thymus and in the spleen), and partially of B-cells (in the spleen). Some lack of B-cells in the bone marrow was connected with a nonspecific action of cyclophosphamide. Cyclophosphamide is supposed to selectively eliminate cells proliferating in response to the antigen.", "contents": "[Immunologic competence of T- and B-lymphocytes in tolerance induced by cyclophosphamide]. A study was made of immunological competence of T- and B-lymphocytes of mice subjected to tolerogenic treatment (administration of a massive dose of sheep erythrocytes and cyclophosphamide 7 days before the experiment). The capacity of lymphocytes of tolerant mice to influence the interaction of normal T- and B-lymphocytes was also investigated. This form of tolerance was caused not by T-suppressors, but by a true deficiency of T-cells-helpers (both in the thymus and in the spleen), and partially of B-cells (in the spleen). Some lack of B-cells in the bone marrow was connected with a nonspecific action of cyclophosphamide. Cyclophosphamide is supposed to selectively eliminate cells proliferating in response to the antigen."}
{"id": "PMID:1084176", "title": "[Kidney ultrastructure in experimental lithiasis and nephrocalcinosis].", "content": "An experimental electron-microscopic study of the kidneys was carried out in experimental oxamide nephrolithiasis in rabbits and hypervitaminosis D in rats. The most pronounced changes were revealed in the proximal and the distal convoluted tubules. It is suggested that cytosomes and lysosome-like bodies possibly participated in stone formation and nephrocalcinosis. It is supposed that they played an important role in the morphogenesis of nephrolithiasis man.", "contents": "[Kidney ultrastructure in experimental lithiasis and nephrocalcinosis]. An experimental electron-microscopic study of the kidneys was carried out in experimental oxamide nephrolithiasis in rabbits and hypervitaminosis D in rats. The most pronounced changes were revealed in the proximal and the distal convoluted tubules. It is suggested that cytosomes and lysosome-like bodies possibly participated in stone formation and nephrocalcinosis. It is supposed that they played an important role in the morphogenesis of nephrolithiasis man."}
{"id": "PMID:1084177", "title": "Immune function at diagnosis in relation to responses to therapy in acute lymphocytic leukemia of childhood.", "content": "Tests of immune capacity were performed on blood from 49 children with newly diagnosed, untreated acute lymphocytic leukemia, and relation to prognosis was determined. Patients were treated with multiple-drug therapy and prophylactic cranial irradiation. Median follow-up time was 16 mo (range 10--37 mo). Principal unfavorable findings at diagnosis were absolute numbers of T lymphoid cells outside the range 850--2500/mul blood, absence of whole blood responses to phytohemagglutinin in vitro, a low titer of complexed antibody, and the presence in serum of free leukemic blast cell membrane antigen. Fourteen patients showed two or more unfavorable findings at diagnosis. Eleven of these have died. Four of the remaining 35 patients have died. A shorter duration of first remission was found among patients with abnormal numbers of T cells at diagnosis. The findings suggest that the immunologic capacity of the patient at diagnosis is an important determinant in responses to therapy.", "contents": "Immune function at diagnosis in relation to responses to therapy in acute lymphocytic leukemia of childhood. Tests of immune capacity were performed on blood from 49 children with newly diagnosed, untreated acute lymphocytic leukemia, and relation to prognosis was determined. Patients were treated with multiple-drug therapy and prophylactic cranial irradiation. Median follow-up time was 16 mo (range 10--37 mo). Principal unfavorable findings at diagnosis were absolute numbers of T lymphoid cells outside the range 850--2500/mul blood, absence of whole blood responses to phytohemagglutinin in vitro, a low titer of complexed antibody, and the presence in serum of free leukemic blast cell membrane antigen. Fourteen patients showed two or more unfavorable findings at diagnosis. Eleven of these have died. Four of the remaining 35 patients have died. A shorter duration of first remission was found among patients with abnormal numbers of T cells at diagnosis. The findings suggest that the immunologic capacity of the patient at diagnosis is an important determinant in responses to therapy."}
{"id": "PMID:1084178", "title": "Periodic oscillation of blood leukocytes, platelets, and reticulocytes in a patient with chronic myelocytic leukemia.", "content": "A patient with chronic myelocytic leukemia had a cyclic oscillation of blood neutrophils, eosinophils, monocytes, platelets, normoblasts, and reticulocytes but not of lymphocytes. The cycle interval was 53--69 days. Except for reticulocytes all other cells cycled with neutrophils. Plasma colony-stimulating factor (CSF) oscillated out of phase with neutrophils, suggesting that granulocytopoiesis is regulated through CSF by a feed-back mechanism. Plasma erythropoiesis-stimulating factor (ESF) also oscillated. ESF crests preceded or coincided with reticulocyte crests, indicating that the ESF elevation may have been responsible for the reticulocyte peaks. The relationship between neutrophils and reticulocytes and their oscillations with plasma CSF and ESF suggests that there is a common stem cell which differentiates along one cell line or the other depending upon the balance of regulatory stimuli. The fraction of blood neutrophilic precursors (myeloblasts, promyelocytes, and myelocytes) in DNA synthesis fluctuated with neutrophil level. The calculated generation time was shorter at the crests than at the troughs of the neutrophil cycles. This finding suggested that the rate of proliferation of the neutrophils changed periodically. This observation, along with a periodic increase in differentiation of the stem cell toward the neutrophilic cells, is the probable explanation of oscillation of the neutrophil count in the blood.", "contents": "Periodic oscillation of blood leukocytes, platelets, and reticulocytes in a patient with chronic myelocytic leukemia. A patient with chronic myelocytic leukemia had a cyclic oscillation of blood neutrophils, eosinophils, monocytes, platelets, normoblasts, and reticulocytes but not of lymphocytes. The cycle interval was 53--69 days. Except for reticulocytes all other cells cycled with neutrophils. Plasma colony-stimulating factor (CSF) oscillated out of phase with neutrophils, suggesting that granulocytopoiesis is regulated through CSF by a feed-back mechanism. Plasma erythropoiesis-stimulating factor (ESF) also oscillated. ESF crests preceded or coincided with reticulocyte crests, indicating that the ESF elevation may have been responsible for the reticulocyte peaks. The relationship between neutrophils and reticulocytes and their oscillations with plasma CSF and ESF suggests that there is a common stem cell which differentiates along one cell line or the other depending upon the balance of regulatory stimuli. The fraction of blood neutrophilic precursors (myeloblasts, promyelocytes, and myelocytes) in DNA synthesis fluctuated with neutrophil level. The calculated generation time was shorter at the crests than at the troughs of the neutrophil cycles. This finding suggested that the rate of proliferation of the neutrophils changed periodically. This observation, along with a periodic increase in differentiation of the stem cell toward the neutrophilic cells, is the probable explanation of oscillation of the neutrophil count in the blood."}
{"id": "PMID:1084197", "title": "A comparison of the time course of excitation and inhibition by iontophoretic decamethonium in frog endplate.", "content": "1 The depolarization of frog endplate by a brief iontophoretic application of decamethonium was slower in time course than the inhibition of a long carbachol response produced by the same decamethonium pulse, or than the excitation produced by a brief equipotent carbachol pulse. 2 The delay between the peak inhibition and peak excitation produced by decamethonium, about 50 ms, is too great to be explained by slow receptor activation kinetics, since Katz & Miledi (1973) have shown that the lifetime of decamethonium-activated receptors is only 0.25 milliseconds. 3 If doses of carbachol and decamethonium are adjusted to give response amplitudes in the ratio corresponding to the ratio of their presumed maximum responses, then there is little difference in the time courses of the responses. 4 This observation, together with the finding that increasing the dose applied slows the decamethonium response much more than the carbachol response, suggests that a decamethonium response contains contributions from a much wider area of receptive membrane than does a carbachol response of equal amplitude. 5 Simulation shows that these geometrical effects are sufficient to account for the rapidity of inhibition compared to excitation without postulating slow receptor kinetics. 6 It is pointed out that similar effects may account for certain results obtained in iontophoretic studies of desensitization.", "contents": "A comparison of the time course of excitation and inhibition by iontophoretic decamethonium in frog endplate. 1 The depolarization of frog endplate by a brief iontophoretic application of decamethonium was slower in time course than the inhibition of a long carbachol response produced by the same decamethonium pulse, or than the excitation produced by a brief equipotent carbachol pulse. 2 The delay between the peak inhibition and peak excitation produced by decamethonium, about 50 ms, is too great to be explained by slow receptor activation kinetics, since Katz & Miledi (1973) have shown that the lifetime of decamethonium-activated receptors is only 0.25 milliseconds. 3 If doses of carbachol and decamethonium are adjusted to give response amplitudes in the ratio corresponding to the ratio of their presumed maximum responses, then there is little difference in the time courses of the responses. 4 This observation, together with the finding that increasing the dose applied slows the decamethonium response much more than the carbachol response, suggests that a decamethonium response contains contributions from a much wider area of receptive membrane than does a carbachol response of equal amplitude. 5 Simulation shows that these geometrical effects are sufficient to account for the rapidity of inhibition compared to excitation without postulating slow receptor kinetics. 6 It is pointed out that similar effects may account for certain results obtained in iontophoretic studies of desensitization."}
{"id": "PMID:1084198", "title": "The efficacy of Alcoholics Anonymous: the elusiveness of hard data.", "content": "The paper is concerned with methodological problems relating to the scientific study of the effectiveness of Alcoholics Anonymous (AA). Studies of the effectiveness of AA fall into two categories--longitudinal and cross-sectional, and may be criticized on the basis of over-simple criteria of success. The particular problems of the requirement for control groups in studies of effectiveness are pointed out. The problems arising out of the whole process of affiliation and disaffiliation and their implications for scientific study are discussed. The requirement for sound statistical analysis is stressed, and inadequacies in the techniques of follow-up are indicated. The studies which have some bearing on AA as a treatment facility are reviewed. These include multivariate studies involving AA attendance as one factor, studies in which AA was the main variable in a hospital programme, and longitudinal studies of AA from within the organization. Particular difficulties in using cross-sectional surveys for the purpose of estimating efficacy are pointed out. The conclusion reached is that because of the methodological difficulties the totality of these studies dose not add significantly to the knowledge concerning AA which we possess from clinical experience. Moreover, without a change in the unusual characteristics of AA when considered as a treatment facility it seems unlikely to be possible to assess its effectiveness in a scientific manner.", "contents": "The efficacy of Alcoholics Anonymous: the elusiveness of hard data. The paper is concerned with methodological problems relating to the scientific study of the effectiveness of Alcoholics Anonymous (AA). Studies of the effectiveness of AA fall into two categories--longitudinal and cross-sectional, and may be criticized on the basis of over-simple criteria of success. The particular problems of the requirement for control groups in studies of effectiveness are pointed out. The problems arising out of the whole process of affiliation and disaffiliation and their implications for scientific study are discussed. The requirement for sound statistical analysis is stressed, and inadequacies in the techniques of follow-up are indicated. The studies which have some bearing on AA as a treatment facility are reviewed. These include multivariate studies involving AA attendance as one factor, studies in which AA was the main variable in a hospital programme, and longitudinal studies of AA from within the organization. Particular difficulties in using cross-sectional surveys for the purpose of estimating efficacy are pointed out. The conclusion reached is that because of the methodological difficulties the totality of these studies dose not add significantly to the knowledge concerning AA which we possess from clinical experience. Moreover, without a change in the unusual characteristics of AA when considered as a treatment facility it seems unlikely to be possible to assess its effectiveness in a scientific manner."}
{"id": "PMID:1084199", "title": "Osteomalacia and carcinoma of prostate with major redistribution of skeletal calcium.", "content": "A case of hypophosphataemic osteomalacia occurring in association with a carcinoma of prostate is described. Although only palliative treatment to the primary tumour was possible, worthwhile remission of bone symptoms, due to osteomalacia, was achieved with pharmacological doses of vitamin D. The presence of extensive skeletal metastases modified the radiological features of osteomalacia. Major alterations in the distribution of calcium within the skeleton were observed during a period when total body calcium remained unaltered. This observation may be of relevance to other cases in which osteosclerotic metastases develop.", "contents": "Osteomalacia and carcinoma of prostate with major redistribution of skeletal calcium. A case of hypophosphataemic osteomalacia occurring in association with a carcinoma of prostate is described. Although only palliative treatment to the primary tumour was possible, worthwhile remission of bone symptoms, due to osteomalacia, was achieved with pharmacological doses of vitamin D. The presence of extensive skeletal metastases modified the radiological features of osteomalacia. Major alterations in the distribution of calcium within the skeleton were observed during a period when total body calcium remained unaltered. This observation may be of relevance to other cases in which osteosclerotic metastases develop."}
{"id": "PMID:1084200", "title": "The ventricular-brain ratio using planimetric measurement of EMI scans.", "content": "A correlation has been shown between the cross-sectional area of the cerebral ventricular system and the cross-sectional area of the brain in 62 patients, using the EMI Scanner. The percentage ratio has been abbreviated to VBR. The patients were divided into normal, equivocal, cerebral atrophy and hydrocephalus, according to their PEG's. The measurements were obtained by the use of a plaimeter and by outlining the ventricular and cerebral perimeters from the EMI Polaroid pictures. The VBR was found to be approximately five in those patients without detectable pathology, about seven in equivocal cases, and above ten in abnormal cases (atrophy, hydrocephalus). The hydrocephalic readings were generally higher than those for cerebral atrophy.", "contents": "The ventricular-brain ratio using planimetric measurement of EMI scans. A correlation has been shown between the cross-sectional area of the cerebral ventricular system and the cross-sectional area of the brain in 62 patients, using the EMI Scanner. The percentage ratio has been abbreviated to VBR. The patients were divided into normal, equivocal, cerebral atrophy and hydrocephalus, according to their PEG's. The measurements were obtained by the use of a plaimeter and by outlining the ventricular and cerebral perimeters from the EMI Polaroid pictures. The VBR was found to be approximately five in those patients without detectable pathology, about seven in equivocal cases, and above ten in abnormal cases (atrophy, hydrocephalus). The hydrocephalic readings were generally higher than those for cerebral atrophy."}
{"id": "PMID:1084201", "title": "Emergency portasystemic shunt for bleeding varices.", "content": "Sixty-eight patients with bleeding oesophageal varices due to cirrhosis of the liver who have had an emergency portasystemic shunt are reviewed. The reasons for the emergency shunt surgery and the results are described and discussed. The low postoperative mortality is attributed to careful case selection. Apart from clinical and serological factors, the importance of the bromsulphthalein excretion test is stressed. Emergency shunts are now undertaken by the author in patients whose serum bilirubin is less than 2-5/100 ml and bromsulphthalein retention less than 10 per cent in 30 minutes. This is in marked contrast to the author's previous series in which there was a 30 per cent mortality. The poor results in patients treated conservatively and the disparity between the author's results and those of other reported series are reviewed and discussed.", "contents": "Emergency portasystemic shunt for bleeding varices. Sixty-eight patients with bleeding oesophageal varices due to cirrhosis of the liver who have had an emergency portasystemic shunt are reviewed. The reasons for the emergency shunt surgery and the results are described and discussed. The low postoperative mortality is attributed to careful case selection. Apart from clinical and serological factors, the importance of the bromsulphthalein excretion test is stressed. Emergency shunts are now undertaken by the author in patients whose serum bilirubin is less than 2-5/100 ml and bromsulphthalein retention less than 10 per cent in 30 minutes. This is in marked contrast to the author's previous series in which there was a 30 per cent mortality. The poor results in patients treated conservatively and the disparity between the author's results and those of other reported series are reviewed and discussed."}
{"id": "PMID:1084202", "title": "Complications of Meckel's diverticulum.", "content": "This paper reviews the presentation and surgical pathology of 50 Meckel's diverticula encountered in one hospital in a 20-year period. It is concluded that in children with intestinal bleeding or a \"raspberry tumour\" of the umbilicus a Meckel's diverticulum is usually suspected. In adults with an acute surgical emergency Meckel's diverticulum is unlikely to be considered. However, a Meckel's diverticulum can give rise to surgical problems in many ways and at any age. In particular, the possiblity of an attached mesodiverticular band leading to obstruction must be stressed.", "contents": "Complications of Meckel's diverticulum. This paper reviews the presentation and surgical pathology of 50 Meckel's diverticula encountered in one hospital in a 20-year period. It is concluded that in children with intestinal bleeding or a \"raspberry tumour\" of the umbilicus a Meckel's diverticulum is usually suspected. In adults with an acute surgical emergency Meckel's diverticulum is unlikely to be considered. However, a Meckel's diverticulum can give rise to surgical problems in many ways and at any age. In particular, the possiblity of an attached mesodiverticular band leading to obstruction must be stressed."}
{"id": "PMID:1084203", "title": "The bleeding caecal angioma: a diagnostic problem.", "content": "Three cases are reported of bleeding angioma of the caecum. The wider use and improved technique of selective arteriography have led to an increase in frequency of diagnosis of this condition, and facilitate the management.", "contents": "The bleeding caecal angioma: a diagnostic problem. Three cases are reported of bleeding angioma of the caecum. The wider use and improved technique of selective arteriography have led to an increase in frequency of diagnosis of this condition, and facilitate the management."}
{"id": "PMID:1084204", "title": "Surgical treatment of bronchiectasis in Kartagener's syndrome.", "content": "A case of Kartagener's syndrome in which bilateral partial pulmonary resection was performed is reported. The literature concerning the surgical treatment of severe bronchiectasis is reviewed.", "contents": "Surgical treatment of bronchiectasis in Kartagener's syndrome. A case of Kartagener's syndrome in which bilateral partial pulmonary resection was performed is reported. The literature concerning the surgical treatment of severe bronchiectasis is reviewed."}
{"id": "PMID:1084206", "title": "Dual localization of histamine in an ascending neuronal pathway and in non-neuronal cells evidenced by lesions in the lateral hypothalamic area.", "content": "The effects of lesions placed in the lateral hypothalamic area, i.e., interrupting the MFB (as evidenced by a 65% decrease in cortical noradrenaline and serotonin) suggest a pluricompartmentation of brain histamine (HA). The existence of an ascending histaminergic system is indicated by the reductions in l-histidine decarboxylase (H.D.) activity, in [3H]histamine synthesis and in HA content, in the cortex of lesioned rats. Moreover, the decrease in H.D. activity was restricted to the regions rostral to the lesion, without modification caudally. The time-course of the alterations in H.D. activity and in HA content is compatible with a process of anterograde degeneration. In subcellular fractionation studies, the reduction in cortical HA content was found to be mainly confined to the P2 fraction, which contains the synaptosomes. Although the widespread ipsilateral distribution of HA synthesizing terminals resembles that of monoaminergic ones, the absence of reduction in H.D. activity after selective destruction of catecholaminergic and serotoninergic neurons, by 6-hydroxydopamine or 5,6-dihydroxytryptamine respectively, renders likely the existence of specific HA-containing neurons. That the release of the amine from these neurons might be related to the nerve impulse flow is suggested by the transient effects of the lesions which preceded the degenerative process (elevated endogenous HA level and slowed rate of [3H]HA synthesis). In addition, the discrepancy between the reduction in H.D. activity and in HA level after the lesions could be explained by the presence of the amine in another neuronal system and/or in non-neuronal cells, not affected by the lesion. This additional compartment is characterized by a high HA content and a low H.D. activity and could therefore be localized in mast-cells. The respective sizes of these two compartments, estimated by several methods, appear to be approximately the same.", "contents": "Dual localization of histamine in an ascending neuronal pathway and in non-neuronal cells evidenced by lesions in the lateral hypothalamic area. The effects of lesions placed in the lateral hypothalamic area, i.e., interrupting the MFB (as evidenced by a 65% decrease in cortical noradrenaline and serotonin) suggest a pluricompartmentation of brain histamine (HA). The existence of an ascending histaminergic system is indicated by the reductions in l-histidine decarboxylase (H.D.) activity, in [3H]histamine synthesis and in HA content, in the cortex of lesioned rats. Moreover, the decrease in H.D. activity was restricted to the regions rostral to the lesion, without modification caudally. The time-course of the alterations in H.D. activity and in HA content is compatible with a process of anterograde degeneration. In subcellular fractionation studies, the reduction in cortical HA content was found to be mainly confined to the P2 fraction, which contains the synaptosomes. Although the widespread ipsilateral distribution of HA synthesizing terminals resembles that of monoaminergic ones, the absence of reduction in H.D. activity after selective destruction of catecholaminergic and serotoninergic neurons, by 6-hydroxydopamine or 5,6-dihydroxytryptamine respectively, renders likely the existence of specific HA-containing neurons. That the release of the amine from these neurons might be related to the nerve impulse flow is suggested by the transient effects of the lesions which preceded the degenerative process (elevated endogenous HA level and slowed rate of [3H]HA synthesis). In addition, the discrepancy between the reduction in H.D. activity and in HA level after the lesions could be explained by the presence of the amine in another neuronal system and/or in non-neuronal cells, not affected by the lesion. This additional compartment is characterized by a high HA content and a low H.D. activity and could therefore be localized in mast-cells. The respective sizes of these two compartments, estimated by several methods, appear to be approximately the same."}
{"id": "PMID:1084209", "title": "Protein metabolism in transected peripheral nerves of the crayfish.", "content": "The significance of the protein metabolism in crayfish peripheral nerve was studied in relation the ability of crayfish motor axons to survive for over 200 days following axotomy. In contrast to frog peripheral nerves, the crayfish nerves appear to more closely resemble ganglia in their profiles of synthesis expressed on sodium dodecyl sulfate (SDS) gels, and have higher incorporation rates of [3H]leucine into protein than ganglia. Since anisomycin inhibits over 95% of protein synthesis in crayfish peripheral nerve, it was concluded that this local protein synthesis was dependent upon a eukaryotic ribosomal mechanism. Radioautography of isolated nerves reveals newly synthesized proteins in glial sheaths, and also within the axoplasm of large motor fibers. Based upon the data available at present, a hypothesis that the glia surrounding the axons are responsible for the local protein synthesis, and that some of these newly synthesized proteins are transported into the axon, is presented. Transection of crayfish peripheral nerves proximal to the neuron cell bodies produced a more than two-fold increase in [3H]leucine incorporation, but no significant changes in labeling profiles of the proteins on SDS gels. The data suggest that while an active local protein synthesis may be necessary for the maintenance of several crayfish motor axons, it is not a sufficient condition.", "contents": "Protein metabolism in transected peripheral nerves of the crayfish. The significance of the protein metabolism in crayfish peripheral nerve was studied in relation the ability of crayfish motor axons to survive for over 200 days following axotomy. In contrast to frog peripheral nerves, the crayfish nerves appear to more closely resemble ganglia in their profiles of synthesis expressed on sodium dodecyl sulfate (SDS) gels, and have higher incorporation rates of [3H]leucine into protein than ganglia. Since anisomycin inhibits over 95% of protein synthesis in crayfish peripheral nerve, it was concluded that this local protein synthesis was dependent upon a eukaryotic ribosomal mechanism. Radioautography of isolated nerves reveals newly synthesized proteins in glial sheaths, and also within the axoplasm of large motor fibers. Based upon the data available at present, a hypothesis that the glia surrounding the axons are responsible for the local protein synthesis, and that some of these newly synthesized proteins are transported into the axon, is presented. Transection of crayfish peripheral nerves proximal to the neuron cell bodies produced a more than two-fold increase in [3H]leucine incorporation, but no significant changes in labeling profiles of the proteins on SDS gels. The data suggest that while an active local protein synthesis may be necessary for the maintenance of several crayfish motor axons, it is not a sufficient condition."}
{"id": "PMID:1084211", "title": "Muscle fiber capacity in low conductivity solution.", "content": "A method is described for computing the effective capacity of muscle fibers, C = Q/V where Q is the charge stored, and V is the membrane potential, using a standard two-microelectrode, constant current injection technique. The method is used to compare physical (or effective) capacity of frog muscle fibers bathed in a low conductivity, 2.5 mMK+ solution, with circuittheory derived quantities in the same cells and in control fibers. No differences can be discerned and it is concluded that low conductivity of physiological solutions, per se, does not significantly reduce the length constant of frog muscle transverse tubules.", "contents": "Muscle fiber capacity in low conductivity solution. A method is described for computing the effective capacity of muscle fibers, C = Q/V where Q is the charge stored, and V is the membrane potential, using a standard two-microelectrode, constant current injection technique. The method is used to compare physical (or effective) capacity of frog muscle fibers bathed in a low conductivity, 2.5 mMK+ solution, with circuittheory derived quantities in the same cells and in control fibers. No differences can be discerned and it is concluded that low conductivity of physiological solutions, per se, does not significantly reduce the length constant of frog muscle transverse tubules."}
{"id": "PMID:1084212", "title": "Myocardial glycogen in prevention of perioperative ischemic injury of the heart: a preliminary report.", "content": "The incidence of arrhythmias, low output and acute myocardial infarction in the perioperative period was analyzed for 119 patients who underwent aortocoronary bypass surgery. The incidence for three groups of patients was as follows: 44% for 72 patients who did not receive glucose-insulin-potassium (GIK) before operation (no-GIK group), 32% for 25 patients who received preoperative GIK alone before operation (GIK group) and zero in 22 patients who received GIK before operation plus a bolus of 50% glucose (0.5 ml/kg body weight) at the beginning of operation (GIK-G group) (the difference between the GIK-G group and the other two groups is significant; P less than 0.001). Determination of myocardial glycogen content of 63 ventricular biopsies revealed a 2+ to 4+ content in 48% of the no-GIK group, 83% of the GIK group and 100% of the GIK-G group (P less than 0.05). The incidence of complications in patients with myocardial glycogen content of 2+ to 4+ was significantly lower (P less than 0.005) than in those with myocardial glycogen content of 1+. Experimentally, myocardial tolerance to ischemic injury parallels myocardial glycogen content. Our preliminary observation that prior administration of GIK-G increases myocardial glycogen content with a concomitant reduction in complications is consistent with these experimental observations.", "contents": "Myocardial glycogen in prevention of perioperative ischemic injury of the heart: a preliminary report. The incidence of arrhythmias, low output and acute myocardial infarction in the perioperative period was analyzed for 119 patients who underwent aortocoronary bypass surgery. The incidence for three groups of patients was as follows: 44% for 72 patients who did not receive glucose-insulin-potassium (GIK) before operation (no-GIK group), 32% for 25 patients who received preoperative GIK alone before operation (GIK group) and zero in 22 patients who received GIK before operation plus a bolus of 50% glucose (0.5 ml/kg body weight) at the beginning of operation (GIK-G group) (the difference between the GIK-G group and the other two groups is significant; P less than 0.001). Determination of myocardial glycogen content of 63 ventricular biopsies revealed a 2+ to 4+ content in 48% of the no-GIK group, 83% of the GIK group and 100% of the GIK-G group (P less than 0.05). The incidence of complications in patients with myocardial glycogen content of 2+ to 4+ was significantly lower (P less than 0.005) than in those with myocardial glycogen content of 1+. Experimentally, myocardial tolerance to ischemic injury parallels myocardial glycogen content. Our preliminary observation that prior administration of GIK-G increases myocardial glycogen content with a concomitant reduction in complications is consistent with these experimental observations."}
{"id": "PMID:1084215", "title": "Thrombotic thrombocytopenic purpura: a syndrome of intravascular platelet consumption.", "content": "In four of five patients with thrombotic thrombocytopenic purpura (TTP) in whom serial tests of hemostatic function were performed, severe thrombocytopenia, normal plasma fibrinogen concentrations and mildly increased concentrations of fibrinogen/fibrin degradation products were observed. Widespread platelet thrombi were found in arterioles and capillaries. Fibrin could be seen around some of the platelet clumps and was the main component in a small number of the thrombi in two patients. The observations show that TTP is a disorder in which intravascular platelet consumption results in disseminated platelet thrombosis. The coagulation system is apparently activated secondarily to platelet aggregation and variable quantities of fibrin are incorporated into the thrombi. Clinical improvement resulted from combined therapy with corticosteroids, heparin and drugs that suppress platelet function.", "contents": "Thrombotic thrombocytopenic purpura: a syndrome of intravascular platelet consumption. In four of five patients with thrombotic thrombocytopenic purpura (TTP) in whom serial tests of hemostatic function were performed, severe thrombocytopenia, normal plasma fibrinogen concentrations and mildly increased concentrations of fibrinogen/fibrin degradation products were observed. Widespread platelet thrombi were found in arterioles and capillaries. Fibrin could be seen around some of the platelet clumps and was the main component in a small number of the thrombi in two patients. The observations show that TTP is a disorder in which intravascular platelet consumption results in disseminated platelet thrombosis. The coagulation system is apparently activated secondarily to platelet aggregation and variable quantities of fibrin are incorporated into the thrombi. Clinical improvement resulted from combined therapy with corticosteroids, heparin and drugs that suppress platelet function."}
{"id": "PMID:1084217", "title": "Erythrocyte receptors and thymus-associated antigens on human thymocytes, mitogen-induced blasts, and acute leukemia blasts.", "content": "Human thymus cells and blasts from some patients with acute lymphoblastic leukemia (ALL) express similar cell surface properties. This suggests that this type of ALL originates in the thymus or alternatively that these properties of ALL blasts reflect changes occurring during blastogenesis. To test these possibilities, we determined whether mitogen-thymus antigens found on blasts from E+ALL  and on normal human thymocytes. E-rosettes of blood T-lymphocytes dissociated at 37 degrees; in contrast, rosettes formed by E+ALL blasts, human thymocytes, and pokeweed mitogen-stimulated blasts were stable at this temperature. Blasts that formed stable rosettes did not have cytoplasmic Ig, indicating that they were T-lymphoblasts. By immunofluorescence and a radiolabeled antibody assay we demonstrated a thymus antigen(s) that was present on the membrane of E+ALL blasts and on normal thymocytes, but not on normal blood T-lymphocytes [TL-like antigen(s)]. This antigen was not expressed on blasts induced by mitogens. The finding that that mitogen-induced blasts form temperature-stable rosettes, but lack TL-like antigen(s), indicates that this antigen is not required for the expression of E-receptors stable at 37 degrees. The results support the concept that E+ALL results from the malignant transformation of thymus cells.", "contents": "Erythrocyte receptors and thymus-associated antigens on human thymocytes, mitogen-induced blasts, and acute leukemia blasts. Human thymus cells and blasts from some patients with acute lymphoblastic leukemia (ALL) express similar cell surface properties. This suggests that this type of ALL originates in the thymus or alternatively that these properties of ALL blasts reflect changes occurring during blastogenesis. To test these possibilities, we determined whether mitogen-thymus antigens found on blasts from E+ALL  and on normal human thymocytes. E-rosettes of blood T-lymphocytes dissociated at 37 degrees; in contrast, rosettes formed by E+ALL blasts, human thymocytes, and pokeweed mitogen-stimulated blasts were stable at this temperature. Blasts that formed stable rosettes did not have cytoplasmic Ig, indicating that they were T-lymphoblasts. By immunofluorescence and a radiolabeled antibody assay we demonstrated a thymus antigen(s) that was present on the membrane of E+ALL blasts and on normal thymocytes, but not on normal blood T-lymphocytes [TL-like antigen(s)]. This antigen was not expressed on blasts induced by mitogens. The finding that that mitogen-induced blasts form temperature-stable rosettes, but lack TL-like antigen(s), indicates that this antigen is not required for the expression of E-receptors stable at 37 degrees. The results support the concept that E+ALL results from the malignant transformation of thymus cells."}
{"id": "PMID:1084219", "title": "Microtubules associated with nuclear pore complexes and coated pits in the CNS.", "content": "Using a new albumin prefixation technique, microtubules have been observed in close association with the nuclear pores of neurons and glia. Thus, microtubules may be involved in such phenomena as anchoring, migration or rotation of the nucleus or in chemical messenger transport between nucleus and cytoplasm. Microtubules are also seen running close to the coated pits of dendrites. The implications are discussed.", "contents": "Microtubules associated with nuclear pore complexes and coated pits in the CNS. Using a new albumin prefixation technique, microtubules have been observed in close association with the nuclear pores of neurons and glia. Thus, microtubules may be involved in such phenomena as anchoring, migration or rotation of the nucleus or in chemical messenger transport between nucleus and cytoplasm. Microtubules are also seen running close to the coated pits of dendrites. The implications are discussed."}
{"id": "PMID:1084220", "title": "Evidence for corticotropin releasing factor (CRF) synthesis in the preoptic nucleus of Xenopus laevis tadpoles: a preliminary report based on lesion experiments.", "content": "For the study of the hypothalamo-hypophysial system of Xenopus laevis tadpoles, hypothalamic lesions were made by means of the electrocoagulation technique. Lesioning of the ventral region of the preoptic nucleus resulted in a decrease of the number of ACTH cells in the pars distalis of the pituitary gland and in a diminution of the PAS-positive reaction of these cells. In addition, regeneration of the neurosecretory cells of the ventral region of the preoptic nucleus observed 6 weeks after lesioning was accompanied by the reappearance of normal PAS-positive ACTH cells in the pars distalis. It is suggested that the neurosecretory cells of the ventral region of the preoptic nucleus of Xenopus laevis tadpoles are related to the ACTH synthesizing cells, probably by producing CRF.", "contents": "Evidence for corticotropin releasing factor (CRF) synthesis in the preoptic nucleus of Xenopus laevis tadpoles: a preliminary report based on lesion experiments. For the study of the hypothalamo-hypophysial system of Xenopus laevis tadpoles, hypothalamic lesions were made by means of the electrocoagulation technique. Lesioning of the ventral region of the preoptic nucleus resulted in a decrease of the number of ACTH cells in the pars distalis of the pituitary gland and in a diminution of the PAS-positive reaction of these cells. In addition, regeneration of the neurosecretory cells of the ventral region of the preoptic nucleus observed 6 weeks after lesioning was accompanied by the reappearance of normal PAS-positive ACTH cells in the pars distalis. It is suggested that the neurosecretory cells of the ventral region of the preoptic nucleus of Xenopus laevis tadpoles are related to the ACTH synthesizing cells, probably by producing CRF."}
{"id": "PMID:1084234", "title": "Electrophoretic variation of serum alpha1-antitrypsin treated with neuraminidase.", "content": "Purified or crude sera of several different phenotypes of alpha1-antitrypsin were treated with neuraminidase. The mobility of the alpha1-antitrypsin zone was delayed on starch gel electrophoresis following incubation. Treated, M-type alpha1-antitrypsin migrated in a manner similar to the untreated S type. Treated S migrated in a similar way to the untreated Z, and treated Z did so more slowly or cathodally. After exhaustive treatment, alpha1-acidglycoprotein bands appeared near the usual M or F positions. Treated samples still possessed the antigenic activity of alpha1-antitrypsin. The concentration and trypsin-inhibitory capacity were not changed by treatment. Several evaluations and considerations are discussed concerning the metabolism of some types of alpha1-antitrypsin.", "contents": "Electrophoretic variation of serum alpha1-antitrypsin treated with neuraminidase. Purified or crude sera of several different phenotypes of alpha1-antitrypsin were treated with neuraminidase. The mobility of the alpha1-antitrypsin zone was delayed on starch gel electrophoresis following incubation. Treated, M-type alpha1-antitrypsin migrated in a manner similar to the untreated S type. Treated S migrated in a similar way to the untreated Z, and treated Z did so more slowly or cathodally. After exhaustive treatment, alpha1-acidglycoprotein bands appeared near the usual M or F positions. Treated samples still possessed the antigenic activity of alpha1-antitrypsin. The concentration and trypsin-inhibitory capacity were not changed by treatment. Several evaluations and considerations are discussed concerning the metabolism of some types of alpha1-antitrypsin."}
{"id": "PMID:1084238", "title": "Chromosomal breaks in T and B lymphocytes in Fanconi's anemia.", "content": "Three patients with Fanconi's anemia were analyzed for chromosome breaks. T and B cells were separated and grown in tissue culture with PHA and pokeweed antigen to ascertain the rates of breakage in these lymphocytic subpopulations. It has been found that there is no statistically significant difference in breakage rates in T and B lymphocytes. It is postulated that both T and B cells could be involved in the development of leukemia in Fanconi's anemia patients, assuming that chromosome breaks constitute a factor predisposing to the development of malignancy.", "contents": "Chromosomal breaks in T and B lymphocytes in Fanconi's anemia. Three patients with Fanconi's anemia were analyzed for chromosome breaks. T and B cells were separated and grown in tissue culture with PHA and pokeweed antigen to ascertain the rates of breakage in these lymphocytic subpopulations. It has been found that there is no statistically significant difference in breakage rates in T and B lymphocytes. It is postulated that both T and B cells could be involved in the development of leukemia in Fanconi's anemia patients, assuming that chromosome breaks constitute a factor predisposing to the development of malignancy."}
{"id": "PMID:1084239", "title": "C3 metabolism in a patient with deficiency of the second component of complement (C2) and discoid lupus erythematosus.", "content": "A patient with a hereditary deficiency of the second component of complement and discoid lupus erythematosus with features of systemic lupus erythematosus was studied. The propositus had a 9-year history of rash and arthralgia. Transient renal disease had completely resolved; there was a history of seizures. Examination of his serum disclosed antinuclear antibodies but no total haemolytic complement activity. C2 was absent. Serum concentrations of C1s, C3, C5 and C9 were elevated; other complement components were present in normal concentration, including C3 pro-activator. The patient's C3 pro-activator was electrophoretically converted by inulin and four of five lipopolysaccharides, but was poorly converted by aggregated human IgG. Two separate turnover studies with radiolabelled C3 showed fractional catabolic rates of 3-03 and 2-48% of the remaining plasma pool/hr (range of three normals: 1-62-2-18%/hr); and estimated C3 synthetic rates of 2-74 and 2-31 mg/kg/hr (range of three normals: 0-89-1-40 mg/kg/hr). Serum complement profiles of the patient's family demonstrated that the C2 deficiency was inherited as an autosomal codominant. One sibling, homozygous for C2 deficiency, and three other siblings, both parents and one daughter, all heterozygous for C2 deficiency, are in good health. Immunofluorescent studies of the patient's diseased skin exhibited substantial deposits of IgG, IgM, C1q, and C4 but not of later acting complement components, properdin, or C3 proactivator. These studies do not support the notion that inflammation in C3-deficient individuals with lupus erythematosus is mediated by the alternative complement pathway.", "contents": "C3 metabolism in a patient with deficiency of the second component of complement (C2) and discoid lupus erythematosus. A patient with a hereditary deficiency of the second component of complement and discoid lupus erythematosus with features of systemic lupus erythematosus was studied. The propositus had a 9-year history of rash and arthralgia. Transient renal disease had completely resolved; there was a history of seizures. Examination of his serum disclosed antinuclear antibodies but no total haemolytic complement activity. C2 was absent. Serum concentrations of C1s, C3, C5 and C9 were elevated; other complement components were present in normal concentration, including C3 pro-activator. The patient's C3 pro-activator was electrophoretically converted by inulin and four of five lipopolysaccharides, but was poorly converted by aggregated human IgG. Two separate turnover studies with radiolabelled C3 showed fractional catabolic rates of 3-03 and 2-48% of the remaining plasma pool/hr (range of three normals: 1-62-2-18%/hr); and estimated C3 synthetic rates of 2-74 and 2-31 mg/kg/hr (range of three normals: 0-89-1-40 mg/kg/hr). Serum complement profiles of the patient's family demonstrated that the C2 deficiency was inherited as an autosomal codominant. One sibling, homozygous for C2 deficiency, and three other siblings, both parents and one daughter, all heterozygous for C2 deficiency, are in good health. Immunofluorescent studies of the patient's diseased skin exhibited substantial deposits of IgG, IgM, C1q, and C4 but not of later acting complement components, properdin, or C3 proactivator. These studies do not support the notion that inflammation in C3-deficient individuals with lupus erythematosus is mediated by the alternative complement pathway."}
{"id": "PMID:1084240", "title": "Cellular aspects of selective IgA deficiency.", "content": "Five patients with no detectable serum IgA (less than 20 mug/ml) and one patient with low serum IgA were compared to normal subjects. The number of circulating E-RFC was normal as was the lymphocyte DNA synthesis induced by PHA, Con A, and streptokinase-streptodornase. The patients had normal numbers of IgA-bearing lymphocytes and normal or increased numbers of B cells. Purified anti-immunoglobulin antibodies specific for IgG, IgA and IgM induced a normal lymphocyte DNA synthesis as did PWM. The patients' lymphocytes were able in vitro to transform into actively secreting IgA plasmocytes. This transformation was determined by counting the IgA and immunoglobulin-containing cells and then measuring the IgA and IgG secretion in the cultures. In some patients PWM was selectively suppressive in IgA B-cell transformation into IgA secreting cells; in the other patients PWM had no effect on the IgA B-cell differentiation. PWM enhanced the IgG secretion in the patients' cultures as well as IgA and IgG secretion in the normal controls.", "contents": "Cellular aspects of selective IgA deficiency. Five patients with no detectable serum IgA (less than 20 mug/ml) and one patient with low serum IgA were compared to normal subjects. The number of circulating E-RFC was normal as was the lymphocyte DNA synthesis induced by PHA, Con A, and streptokinase-streptodornase. The patients had normal numbers of IgA-bearing lymphocytes and normal or increased numbers of B cells. Purified anti-immunoglobulin antibodies specific for IgG, IgA and IgM induced a normal lymphocyte DNA synthesis as did PWM. The patients' lymphocytes were able in vitro to transform into actively secreting IgA plasmocytes. This transformation was determined by counting the IgA and immunoglobulin-containing cells and then measuring the IgA and IgG secretion in the cultures. In some patients PWM was selectively suppressive in IgA B-cell transformation into IgA secreting cells; in the other patients PWM had no effect on the IgA B-cell differentiation. PWM enhanced the IgG secretion in the patients' cultures as well as IgA and IgG secretion in the normal controls."}
{"id": "PMID:1084241", "title": "T and B lymphocytes and Reed-Sternberg cells in Hodgkin's disease lymph nodes and spleens.", "content": "Lymphoid cells from twenty-four untreated Hodgkin's disease biopsies were examined for spontaneous sheep erythrocyte and sensitized ox erythrocyte rosette formation for the identification of T cell and cells with Fc and C3 receptors and surface immunoglobulin. Compared with normal tissues mean T-lymphocytes values were elevated in both involved lymph nodes and uninvolved spleens from Hodgkin's patients. Lymphocytes bearing C3 receptors were correspondingly reduced in these tissues. Involved spleen T-cell values fell within the normal range. In normal tissues the sum of lymphocytes with surface immunoglobulin and sheep erythrocyte receptors fell in the range 89-108%. In six biopsies of Hodgkin's tissue the sum was outside the normal range (121-142%). This observation is compatible with surface immunoglobulin-coated T cells. Surface marker characteristics and intracellular immunoglobulin studies of small lymphocytes, lymphoblasts and Hodgkin's cells suggested that the neoplastic cells were of B lymphocyte origin.", "contents": "T and B lymphocytes and Reed-Sternberg cells in Hodgkin's disease lymph nodes and spleens. Lymphoid cells from twenty-four untreated Hodgkin's disease biopsies were examined for spontaneous sheep erythrocyte and sensitized ox erythrocyte rosette formation for the identification of T cell and cells with Fc and C3 receptors and surface immunoglobulin. Compared with normal tissues mean T-lymphocytes values were elevated in both involved lymph nodes and uninvolved spleens from Hodgkin's patients. Lymphocytes bearing C3 receptors were correspondingly reduced in these tissues. Involved spleen T-cell values fell within the normal range. In normal tissues the sum of lymphocytes with surface immunoglobulin and sheep erythrocyte receptors fell in the range 89-108%. In six biopsies of Hodgkin's tissue the sum was outside the normal range (121-142%). This observation is compatible with surface immunoglobulin-coated T cells. Surface marker characteristics and intracellular immunoglobulin studies of small lymphocytes, lymphoblasts and Hodgkin's cells suggested that the neoplastic cells were of B lymphocyte origin."}
{"id": "PMID:1084242", "title": "Smooth muscle antibodies in Mycoplasma pneumoniae infection.", "content": "Paired sera from forty-five cases of Mycoplasma pneumoniae (MP) infection associated with acute lower respiratory tract illness were examined by immunofluorescence for antibodies to smooth muscle. Twenty-five (56%) of these cases had smooth muscle antibodies (SMA) of IgM class. A significant (greater than or equal to 4-fold) increase in titre of these antibodies was demonstrated in fifteen of thirty-five patients with a significant rise in titre of MP antibodies. SMA of IgG class occurred in eleven of forty-five cases (24%), but a 4-fold rise in antibody titre was found only in two cases. Three of forty-five sera (7%) from healthy donors contained SMA of IgM class and eight sera (18%) SMA of IgG class. MP antigen did not absorb SMA. Liver tests were performed in twenty-nine patients. In eighteen patients SGPT values were moderately or slightly elevated. There was no correlation between the occurrence of increased levels of transaminases and the presence of SMA in serum. In a patient with active chronic hepatitis, who had had a high titre of SMA exclusively of IgG class for 2 years, SMA of IgM class appeared transiently in association with an acute respiratory illness due to MP.", "contents": "Smooth muscle antibodies in Mycoplasma pneumoniae infection. Paired sera from forty-five cases of Mycoplasma pneumoniae (MP) infection associated with acute lower respiratory tract illness were examined by immunofluorescence for antibodies to smooth muscle. Twenty-five (56%) of these cases had smooth muscle antibodies (SMA) of IgM class. A significant (greater than or equal to 4-fold) increase in titre of these antibodies was demonstrated in fifteen of thirty-five patients with a significant rise in titre of MP antibodies. SMA of IgG class occurred in eleven of forty-five cases (24%), but a 4-fold rise in antibody titre was found only in two cases. Three of forty-five sera (7%) from healthy donors contained SMA of IgM class and eight sera (18%) SMA of IgG class. MP antigen did not absorb SMA. Liver tests were performed in twenty-nine patients. In eighteen patients SGPT values were moderately or slightly elevated. There was no correlation between the occurrence of increased levels of transaminases and the presence of SMA in serum. In a patient with active chronic hepatitis, who had had a high titre of SMA exclusively of IgG class for 2 years, SMA of IgM class appeared transiently in association with an acute respiratory illness due to MP."}
{"id": "PMID:1084243", "title": "Antibody-dependent direct cytotoxicity of human lymphocytes. I. Studies on peripheral blood lymphocytes and sera of patients with systemic lupus erythematosus.", "content": "Antibody-dependent direct cytotoxicity (ADDC) is generally believed to be unrelated to T-cell function in experimental animals. The role of ADDC in humans and its clinical usefulesss was evaluated in patients with systemic lupus erythematosus (SLE) and normal controls. Peripheral blood lymphocytes from patients with active SLE were unable to lyse antibody-coated target cells in vitro to the same degree as lymphocytes from patients with inactive SLE and controls. Sera from patients with active SLE suppressed ADDC by lymphocytes derived from normal controls and this abnormality was not corrected by overnight incubation or by extensive washing of lymphocyte preparations. Although there was poor correlation between ADDC and the proportions of B cells and null cells in effector lymphocyte populations from SLE patients and controls, it is concluded that this assay provides another means of determining immune competence in man.", "contents": "Antibody-dependent direct cytotoxicity of human lymphocytes. I. Studies on peripheral blood lymphocytes and sera of patients with systemic lupus erythematosus. Antibody-dependent direct cytotoxicity (ADDC) is generally believed to be unrelated to T-cell function in experimental animals. The role of ADDC in humans and its clinical usefulesss was evaluated in patients with systemic lupus erythematosus (SLE) and normal controls. Peripheral blood lymphocytes from patients with active SLE were unable to lyse antibody-coated target cells in vitro to the same degree as lymphocytes from patients with inactive SLE and controls. Sera from patients with active SLE suppressed ADDC by lymphocytes derived from normal controls and this abnormality was not corrected by overnight incubation or by extensive washing of lymphocyte preparations. Although there was poor correlation between ADDC and the proportions of B cells and null cells in effector lymphocyte populations from SLE patients and controls, it is concluded that this assay provides another means of determining immune competence in man."}
{"id": "PMID:1084244", "title": "Antibody-dependent direct cytotoxicity of human lymphocytes. II. Studies on peripheral blood lymphocytes, synovial fluid cells and sera from patients with rheumatoid arthritis.", "content": "Antibody-dependent direct cytotoxicity (ADDC) by human lymphocytes was evaluated in patients with rheumatoid arthritis and normal controls. Purified peripheral blood and synovial fluid lymphocytes mediated normal ADDC when compared to control subjects. No correlation could be obtained between the percentages of T, B and null cells in effector cell populations and the degree of 51Cr released from target cells. Sera from 50% of patients with rheumatoid arthritis inhibited ADDC by normal lymphocytes; the degree of inhibition did not correlate with the titre of IgM rheumatoid factor. The pathogenic implications of these findings are discussed.", "contents": "Antibody-dependent direct cytotoxicity of human lymphocytes. II. Studies on peripheral blood lymphocytes, synovial fluid cells and sera from patients with rheumatoid arthritis. Antibody-dependent direct cytotoxicity (ADDC) by human lymphocytes was evaluated in patients with rheumatoid arthritis and normal controls. Purified peripheral blood and synovial fluid lymphocytes mediated normal ADDC when compared to control subjects. No correlation could be obtained between the percentages of T, B and null cells in effector cell populations and the degree of 51Cr released from target cells. Sera from 50% of patients with rheumatoid arthritis inhibited ADDC by normal lymphocytes; the degree of inhibition did not correlate with the titre of IgM rheumatoid factor. The pathogenic implications of these findings are discussed."}
{"id": "PMID:1084245", "title": "Inhibitory effect of sheep erythrocyte fragments on rosette formation of human T lymphocytes with sheep red blood cells.", "content": "The effect of sheep red blood cells (SRBC) fragments on rosette formation of human peripheral T lymphocytes with SRBC was evaluated on the active and total T-rosette tests. The rosetting capacity of active rosette-forming cells was selectively and nearly completely inhibited by the pretreatment of lymphocytes with SRBC fragments. The decrease in total rosettes by blocking with SRBC fragments was almost parallel to that of active rosettes. SRBC fragments had no inhibitory effect on the rosetting capacity of a lymphocyte population in which active rosette-forming cells were removed by gradient centrifugation. These results suggested that active rosette-forming cells in human T lymphocytes have the receptors of high affinity for SRBC and these receptors readily bind SRBC fragments, resulting in block of rosette formation.", "contents": "Inhibitory effect of sheep erythrocyte fragments on rosette formation of human T lymphocytes with sheep red blood cells. The effect of sheep red blood cells (SRBC) fragments on rosette formation of human peripheral T lymphocytes with SRBC was evaluated on the active and total T-rosette tests. The rosetting capacity of active rosette-forming cells was selectively and nearly completely inhibited by the pretreatment of lymphocytes with SRBC fragments. The decrease in total rosettes by blocking with SRBC fragments was almost parallel to that of active rosettes. SRBC fragments had no inhibitory effect on the rosetting capacity of a lymphocyte population in which active rosette-forming cells were removed by gradient centrifugation. These results suggested that active rosette-forming cells in human T lymphocytes have the receptors of high affinity for SRBC and these receptors readily bind SRBC fragments, resulting in block of rosette formation."}
{"id": "PMID:1084249", "title": "Multiple hereditary osteochondromata. Report of an early case.", "content": "Observations on the early development of multiple osteochondromata in a 3-year-old girl suggest that aberrant growth of a peripheral segment of the growth plate, with extension primarily toward the metaphysis, but towards the epiphysis, impairs development of adjacent areas of epiphyseal cartilage.", "contents": "Multiple hereditary osteochondromata. Report of an early case. Observations on the early development of multiple osteochondromata in a 3-year-old girl suggest that aberrant growth of a peripheral segment of the growth plate, with extension primarily toward the metaphysis, but towards the epiphysis, impairs development of adjacent areas of epiphyseal cartilage."}
{"id": "PMID:1084250", "title": "Gastrointestinal complications of aortic bypass surgery.", "content": "Gastrointestinal bleeding following abdominal aortic bypass surgery is not uncommon, as approximately 20% of patients with abdominal aortic aneurysms have peptic ulcer disease. We have recently seen three patients who presented with gastrointestinal bleeding secondary to the complications of their surgery. The cause of the bleeding was aortoduodenal fistula, graft erosion into the sigmoid colon and ischaemic colitis respectively. The correct diagnosis was only considered in one patient, although in retrospect it should have been suggested in all three. Our experiences with these complications and their clinical and radiological presentation form the basis for this paper.", "contents": "Gastrointestinal complications of aortic bypass surgery. Gastrointestinal bleeding following abdominal aortic bypass surgery is not uncommon, as approximately 20% of patients with abdominal aortic aneurysms have peptic ulcer disease. We have recently seen three patients who presented with gastrointestinal bleeding secondary to the complications of their surgery. The cause of the bleeding was aortoduodenal fistula, graft erosion into the sigmoid colon and ischaemic colitis respectively. The correct diagnosis was only considered in one patient, although in retrospect it should have been suggested in all three. Our experiences with these complications and their clinical and radiological presentation form the basis for this paper."}
{"id": "PMID:1084251", "title": "Release of colony-stimulating activity from the isolated perfused rat liver.", "content": "1. Colony-stimulating activity appeared in the perfusate of the isolated rat liver during perfusions with either whole rat blood, rat plasma or an artificial perfusate of Eagle's medium and albumin. 2. Dialysable inhibitors of colony formation were also released during perfusions. 3. Colony-stimulating activity in artificial perfusate could be enhanced by the addition of rat plasma in vitro. Concentrations of cycloheximide that inhibited albumin synthesis by the liver did not inhibit the release of colony-stimulating activity.", "contents": "Release of colony-stimulating activity from the isolated perfused rat liver. 1. Colony-stimulating activity appeared in the perfusate of the isolated rat liver during perfusions with either whole rat blood, rat plasma or an artificial perfusate of Eagle's medium and albumin. 2. Dialysable inhibitors of colony formation were also released during perfusions. 3. Colony-stimulating activity in artificial perfusate could be enhanced by the addition of rat plasma in vitro. Concentrations of cycloheximide that inhibited albumin synthesis by the liver did not inhibit the release of colony-stimulating activity."}
{"id": "PMID:1084254", "title": "Cardiac tamponade complicating closure of a median sternotomy.", "content": "A case of intraoperative cardiac tamponade manifested during closure of a median sternotomy is presented. We postulate that cardiac tamponade was caused by acute dilatation of the cardiac chambers as a result of intraoartic balloon pumping in a patient with aortic and mitral regurgitation. It has been shown experimentally that acute rises in ventricular end-diastolic pressure result in increased intrapericardial pressure and that if a certain point on the pericardial pressure-volume curve is reached, cardiac tamponade will occur. Sternotomy closure was accomplished easily as soon as the need for intra-aortic balloon pumping diminished.", "contents": "Cardiac tamponade complicating closure of a median sternotomy. A case of intraoperative cardiac tamponade manifested during closure of a median sternotomy is presented. We postulate that cardiac tamponade was caused by acute dilatation of the cardiac chambers as a result of intraoartic balloon pumping in a patient with aortic and mitral regurgitation. It has been shown experimentally that acute rises in ventricular end-diastolic pressure result in increased intrapericardial pressure and that if a certain point on the pericardial pressure-volume curve is reached, cardiac tamponade will occur. Sternotomy closure was accomplished easily as soon as the need for intra-aortic balloon pumping diminished."}
{"id": "PMID:1084264", "title": "[Ulcerative colitis and pregnancy. Surgical treatment of acute complications (author's transl)].", "content": "Ulcerative colitis occurs chiefly in women in the reproductive age group. The disease tends to exacerbate in affected patients with florid colitis at time of conception. Toxic complications and severe haemorrhage are indications for surgical intervention at the earliest possible time. Treatment of choice consists of multiple enterostomies. Two female patients could be saved with this method. Proctocolectomies were always unsuccessful.", "contents": "[Ulcerative colitis and pregnancy. Surgical treatment of acute complications (author's transl)]. Ulcerative colitis occurs chiefly in women in the reproductive age group. The disease tends to exacerbate in affected patients with florid colitis at time of conception. Toxic complications and severe haemorrhage are indications for surgical intervention at the earliest possible time. Treatment of choice consists of multiple enterostomies. Two female patients could be saved with this method. Proctocolectomies were always unsuccessful."}
{"id": "PMID:1084262", "title": "Acute hemorrhage from right-colonic hemangiomas: report of a case.", "content": "A rare case of severe rectal bleeding beginning in adult life is reported. A diffuse, cavernous hemangioma of the right transverse colon was visualized by superior mesenteric arteriography and the diagnosis was confirmed by peroperative colonoscopy carried out through a colotomy. The patient was treated by right hemicolectomy. The diagnostic value of selective abdominal arteriography in patients who have severe rectal bleeding is demonstrated.", "contents": "Acute hemorrhage from right-colonic hemangiomas: report of a case. A rare case of severe rectal bleeding beginning in adult life is reported. A diffuse, cavernous hemangioma of the right transverse colon was visualized by superior mesenteric arteriography and the diagnosis was confirmed by peroperative colonoscopy carried out through a colotomy. The patient was treated by right hemicolectomy. The diagnostic value of selective abdominal arteriography in patients who have severe rectal bleeding is demonstrated."}
{"id": "PMID:1084266", "title": "Immunoreactive insulin in bile and pancreatic juice of rat.", "content": "Immunoreactive insulin (IRI) was determined in the bile and pancreatic juice of rats. Recovery studies in which known amounts of insulin were added to samples of these fluids indicated that the IRI values corresponded closely to total recovery. The concentration of IRI in bile and pancreatic juice was greater than the concentration in arterial plasma and less than the concentration in hepatic portal venous plasma adjacent to the liver. Elevation of the circulating IRI concentration in fasting anaesthetized animals by intravenously administered glucose or insulin produced a concomitant increase in the concentration of IRI in bile and pancreatic juice. In fasting conscious animals there was a circadian variation in the concentration of IRI in bile and pancreatic juice similar to the variation of IRI in the circulation. The total amount of IRI in the bile and pancreatic juice of fasting conscious rats during 24 h was in excess of 4 mU/kg body weight. These studies demonstrate that considerable quantities of IRI are transferred from the circulation into the bile and pancreatic juice of rats.", "contents": "Immunoreactive insulin in bile and pancreatic juice of rat. Immunoreactive insulin (IRI) was determined in the bile and pancreatic juice of rats. Recovery studies in which known amounts of insulin were added to samples of these fluids indicated that the IRI values corresponded closely to total recovery. The concentration of IRI in bile and pancreatic juice was greater than the concentration in arterial plasma and less than the concentration in hepatic portal venous plasma adjacent to the liver. Elevation of the circulating IRI concentration in fasting anaesthetized animals by intravenously administered glucose or insulin produced a concomitant increase in the concentration of IRI in bile and pancreatic juice. In fasting conscious animals there was a circadian variation in the concentration of IRI in bile and pancreatic juice similar to the variation of IRI in the circulation. The total amount of IRI in the bile and pancreatic juice of fasting conscious rats during 24 h was in excess of 4 mU/kg body weight. These studies demonstrate that considerable quantities of IRI are transferred from the circulation into the bile and pancreatic juice of rats."}
{"id": "PMID:1084261", "title": "Anorectal melanoma: report of two cases.", "content": "We have described the clinicopathologic findings in two cases of anorectal melanoma, and extracted the salient features from the medical literature. The disease is rare. Melanoma arises from the anal squamous membrane and very often spreads upward through submucosal planes, producing secondary satelites in the rectum. Trauma from defecation, vast lymphatic and venous systems in the anorectal region, and high invasiveness of the tumor cells eviden;ly account for early distant metastases. Histologically, the neoplastic cells often mimic other cancers. Treatment is surgical, with dismal end results.", "contents": "Anorectal melanoma: report of two cases. We have described the clinicopathologic findings in two cases of anorectal melanoma, and extracted the salient features from the medical literature. The disease is rare. Melanoma arises from the anal squamous membrane and very often spreads upward through submucosal planes, producing secondary satelites in the rectum. Trauma from defecation, vast lymphatic and venous systems in the anorectal region, and high invasiveness of the tumor cells eviden;ly account for early distant metastases. Histologically, the neoplastic cells often mimic other cancers. Treatment is surgical, with dismal end results."}
{"id": "PMID:1084267", "title": "Interrelation between anterior pituitary weight and ceruloplasmin level in serum after estrogen administration in male rats.", "content": "Male rats were injected 11 mg estradiol benzoate in microcrystalline water suspension i. m. twice a week for three weeks. A significant correlation was found between the weight of anterior pituitary and ceruloplasmin level in serum (r = 0.358; P less than 0.01).", "contents": "Interrelation between anterior pituitary weight and ceruloplasmin level in serum after estrogen administration in male rats. Male rats were injected 11 mg estradiol benzoate in microcrystalline water suspension i. m. twice a week for three weeks. A significant correlation was found between the weight of anterior pituitary and ceruloplasmin level in serum (r = 0.358; P less than 0.01)."}
{"id": "PMID:1084268", "title": "Diurnal variation in the effect of melatonin on plasma and muscle free fatty acid levels in the pigeon.", "content": "Pigeons maintained on standard diet and held under 12 h daily photo-period in a controlled environmental room, were given intravenous injections of melatonin. A low dose (1.25 mg/kg body weight) of melatonin when given in the middle of the scotophase, produced a significant increase in plasma FFA when estimated at 20 min and 90 min post-injection, whereas no significant change was seen with injections given in the middle of the photophase. No significant change in muscle FFA level was obtained either during the photophase or the scotophase when estimated at 90 min postinjection. With a higher dose (5 mg/kg body weight) of melatonin given in the scotophase, on the other hand, a significant increase in both plasma as well as muscle FFA levels was obtained at 90 min post-injection but there was no effect on plasma FFA at 20 min or 90 min post-injection in the photophase and at 20 min in the scotophase. It is concluded that melatonin has a lipid mobilizing action in the pigeon when administered during the scotophase.", "contents": "Diurnal variation in the effect of melatonin on plasma and muscle free fatty acid levels in the pigeon. Pigeons maintained on standard diet and held under 12 h daily photo-period in a controlled environmental room, were given intravenous injections of melatonin. A low dose (1.25 mg/kg body weight) of melatonin when given in the middle of the scotophase, produced a significant increase in plasma FFA when estimated at 20 min and 90 min post-injection, whereas no significant change was seen with injections given in the middle of the photophase. No significant change in muscle FFA level was obtained either during the photophase or the scotophase when estimated at 90 min postinjection. With a higher dose (5 mg/kg body weight) of melatonin given in the scotophase, on the other hand, a significant increase in both plasma as well as muscle FFA levels was obtained at 90 min post-injection but there was no effect on plasma FFA at 20 min or 90 min post-injection in the photophase and at 20 min in the scotophase. It is concluded that melatonin has a lipid mobilizing action in the pigeon when administered during the scotophase."}
{"id": "PMID:1084269", "title": "Adrenocortical changes in rats during acute and chronic administration of delta-9-tetrahydrocannabinol.", "content": "Acute intraperitoneal administration of delta-9-THC (at doses of 10 mg/kg and 50 mg/kg) to adult male albino rats showed characteristic stimulating effect on adrenocortical tissues with marked depletion of ascorbic acid, cholesterol containing neutral lipids and significant increase in the delta5-3 beta -hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase activities in the zona fasciculata and zona reticularis regions. Adrenal weights and the histometric measurements of the adrenocortical areas showed no significant changes. Chronic intraperitoneal administration of delta-9-THC (at doses of 10 mg/kg per day for 15 days) produced marked accumulation of cholesterol containing neutral lipids, with increase in ascorbic acid and delta5-3 beta-hydroxysteroid dehydrogenase and glucose 6-phosphate dehydrogenase activities in the fasciculata-reticularis regions. Adrenal weights and histometric measurements revealed marked hypertrophy of the adrenal glands.", "contents": "Adrenocortical changes in rats during acute and chronic administration of delta-9-tetrahydrocannabinol. Acute intraperitoneal administration of delta-9-THC (at doses of 10 mg/kg and 50 mg/kg) to adult male albino rats showed characteristic stimulating effect on adrenocortical tissues with marked depletion of ascorbic acid, cholesterol containing neutral lipids and significant increase in the delta5-3 beta -hydroxysteroid dehydrogenase and glucose-6-phosphate dehydrogenase activities in the zona fasciculata and zona reticularis regions. Adrenal weights and the histometric measurements of the adrenocortical areas showed no significant changes. Chronic intraperitoneal administration of delta-9-THC (at doses of 10 mg/kg per day for 15 days) produced marked accumulation of cholesterol containing neutral lipids, with increase in ascorbic acid and delta5-3 beta-hydroxysteroid dehydrogenase and glucose 6-phosphate dehydrogenase activities in the fasciculata-reticularis regions. Adrenal weights and histometric measurements revealed marked hypertrophy of the adrenal glands."}
{"id": "PMID:1084270", "title": "Investigation of luteinizing-inducing effect of pinealectomy or thyroidectomy in different types of anovulatory syndromes in rats.", "content": "The luteinizing-inducing effect of pinealectomy and of thyroidectomy was investigated in the constant estrous anovulatory (CEA) syndrome caused either by frontal hypothalamic deafferentation (FHD) or by neonatal androgen treatment (NA). Thyroidectomy failed to induce luteinization in both types of CEA syndrome. However, pinealectomy provoked the formation of corpora lutea in FHD, but not in NA induced CEA syndrome. It was concluded that the luteinizing-inducing effect of pinealectomy is a specific consequence of the removal of the pineal hormone-like principles. The differences in the mechanism of development of the CEA syndrome following FHD or NA might account for the failure of pinealectomy to elicite luteinization in the NA induced CEA rat.", "contents": "Investigation of luteinizing-inducing effect of pinealectomy or thyroidectomy in different types of anovulatory syndromes in rats. The luteinizing-inducing effect of pinealectomy and of thyroidectomy was investigated in the constant estrous anovulatory (CEA) syndrome caused either by frontal hypothalamic deafferentation (FHD) or by neonatal androgen treatment (NA). Thyroidectomy failed to induce luteinization in both types of CEA syndrome. However, pinealectomy provoked the formation of corpora lutea in FHD, but not in NA induced CEA syndrome. It was concluded that the luteinizing-inducing effect of pinealectomy is a specific consequence of the removal of the pineal hormone-like principles. The differences in the mechanism of development of the CEA syndrome following FHD or NA might account for the failure of pinealectomy to elicite luteinization in the NA induced CEA rat."}
{"id": "PMID:1084271", "title": "Dynamics of biosynthesis of thyroglobulin subunits and their polymerization in rabbit thyroid slices in vitro.", "content": "The dynamics of biosynthesis and aggregation of subunits into thyroglobulin (TG) was studied in vitro in rabbit thyroid slices incubated from 5 to 300 min in the presence of I-14C leucine. The incorporation of labelled amino acid was followed in total soluble and microsome-bound proteins, as well as its distribution in soluble protein fractions. The incorporation of lebelled amino acid into soluble and microsome-bound proteins increased with time of incubation. The analysis of individual soluble proteins indicated that the label was incorporated very early, not only into 3--8S, but also into a protein which corresponds to the 12S fraction. Maximal incorporation into 12S protein was achieved after 60 min of incubation and then the intensity of incorporation decreased, followed by an increase in the relative and absolute amount of TG. The appearance of 14C-leucine in the TG region was not observed before 30 min of incubation. The dynamics of incorporation of 14C-leucine into thyroid proteins indicates very rapid transformation of newly synthesized 12S subunits into TG. After two hours of incubation the newly synthesized TG already showed the same sedimentation properties as pre-formed rabbit TG or native rat 19S TG-125I thus demonstrating that maturation, similar to polymerization, is a very rapid process, i. e. the newly formed TG is quickly transformed into its mature form. Our previous studies and the results presented in this work suggest that 12S protein may also have a precursor character. The presence of 12S protein in thyroid extract is not always the result of dissociation of TG, but also a consequence of polymerization of the basic subunits of TG.", "contents": "Dynamics of biosynthesis of thyroglobulin subunits and their polymerization in rabbit thyroid slices in vitro. The dynamics of biosynthesis and aggregation of subunits into thyroglobulin (TG) was studied in vitro in rabbit thyroid slices incubated from 5 to 300 min in the presence of I-14C leucine. The incorporation of labelled amino acid was followed in total soluble and microsome-bound proteins, as well as its distribution in soluble protein fractions. The incorporation of lebelled amino acid into soluble and microsome-bound proteins increased with time of incubation. The analysis of individual soluble proteins indicated that the label was incorporated very early, not only into 3--8S, but also into a protein which corresponds to the 12S fraction. Maximal incorporation into 12S protein was achieved after 60 min of incubation and then the intensity of incorporation decreased, followed by an increase in the relative and absolute amount of TG. The appearance of 14C-leucine in the TG region was not observed before 30 min of incubation. The dynamics of incorporation of 14C-leucine into thyroid proteins indicates very rapid transformation of newly synthesized 12S subunits into TG. After two hours of incubation the newly synthesized TG already showed the same sedimentation properties as pre-formed rabbit TG or native rat 19S TG-125I thus demonstrating that maturation, similar to polymerization, is a very rapid process, i. e. the newly formed TG is quickly transformed into its mature form. Our previous studies and the results presented in this work suggest that 12S protein may also have a precursor character. The presence of 12S protein in thyroid extract is not always the result of dissociation of TG, but also a consequence of polymerization of the basic subunits of TG."}
{"id": "PMID:1084272", "title": "Prolonged oral administration of potassium upon aldosterone biosynthesis by rat glomerulosa tissue in vitro.", "content": "Steroid production rate of adrenals derived from rats drinking a 0.3 M KC1 + 5% glucose solution for 7 days was compared to that of control rats drinking a 5% glucose solution in order to investigate the effect of potassium loading upon the early and late step of aldosterone biosynthesis. Following potassium loading the quartered adrenals produced more aldosterone but less corticosterone as compared to the control. Potassium loading resulted in an increased aldosterone production rate by capsular adrenals (z. glomerulosa) provided that the corticosterone concentration in the incubation medium was elevated either by incubating it together with the decapsulated adrenal or adding exogenous corticosterone (4--16 mug/ml) to the medium. The corticosterone to aldosterone converting capacity of capsular adrenals is markedly higher in the potassium-loaded rats than in the controls. In the first 15 minutes of incubation the corticosterone production rate of the two groups was equal, aldosterone production rate by capsular adrenals of potassium-loaded rats, being higher than that of control animals. Corticosterone output of capsular adrenals from potassium-loaded rats decreased more rapidly in course of the incubation than it did in control tissue. These results suggest that the increase in aldosterone secretion in vivo following potassium loading is due to the stimulation of conversion of corticosterone to aldosterone in the glomerulosa cells. However, the endogenous corticosterone production during the incubation of glomerulosa cells from pottasium-loaded rats decreases so rapidly that the cells are not capable of producing more aldosterone than the control ones in spite of activated 18-hydroxylase.", "contents": "Prolonged oral administration of potassium upon aldosterone biosynthesis by rat glomerulosa tissue in vitro. Steroid production rate of adrenals derived from rats drinking a 0.3 M KC1 + 5% glucose solution for 7 days was compared to that of control rats drinking a 5% glucose solution in order to investigate the effect of potassium loading upon the early and late step of aldosterone biosynthesis. Following potassium loading the quartered adrenals produced more aldosterone but less corticosterone as compared to the control. Potassium loading resulted in an increased aldosterone production rate by capsular adrenals (z. glomerulosa) provided that the corticosterone concentration in the incubation medium was elevated either by incubating it together with the decapsulated adrenal or adding exogenous corticosterone (4--16 mug/ml) to the medium. The corticosterone to aldosterone converting capacity of capsular adrenals is markedly higher in the potassium-loaded rats than in the controls. In the first 15 minutes of incubation the corticosterone production rate of the two groups was equal, aldosterone production rate by capsular adrenals of potassium-loaded rats, being higher than that of control animals. Corticosterone output of capsular adrenals from potassium-loaded rats decreased more rapidly in course of the incubation than it did in control tissue. These results suggest that the increase in aldosterone secretion in vivo following potassium loading is due to the stimulation of conversion of corticosterone to aldosterone in the glomerulosa cells. However, the endogenous corticosterone production during the incubation of glomerulosa cells from pottasium-loaded rats decreases so rapidly that the cells are not capable of producing more aldosterone than the control ones in spite of activated 18-hydroxylase."}
{"id": "PMID:1084273", "title": "Changes in uptake of 3H-progesterone by female rat brain and pituitary from birth to sexual maturity.", "content": "3H-progesterone uptake by various parts of the brain, pituitary and skeletal muscle was compared in newborn, 5-, 10-, 15-, 20-, 25- and 50-day old female rats at 1 hr after a single intraperitoneal injection of 50 muCi/100g body weight. High uptake values in newborn animals and in those aged 5 days were found in all tissues investigated. A sharp decrease in accumulation was observed from birth and/or 5th day of life. The uptake by pituitary was persistantly higher than those by other tissues investigated. The ratio of the concentration of radioactivity between the tissues and the cerebellar cortex increased significantly only in the posterior hypothalamus of adult females (at the age of 50 days). In the pituitary the ratio tissue/cortex was already significantly higher in newborns. The high level of brain radioactivity in the youngest animals is probably a manifestation of high plasma concentrations of tritiated progesterone. The striking decrease in uptake of radioactivity by the brain and pituitary during the first two weeks of life most likely reflects the decrease in level of plasma radioactivity, as shown indirectly by the concomitant decrease in labelled progesterone uptake by the skeletal muscle. The increase in tissue/cortex ratio in the posterior hypothalamus with attainment of sexual maturity suggests the first appearance of specific binding capacity for progesterone which is assumed to be present in the pituitary already from birth.", "contents": "Changes in uptake of 3H-progesterone by female rat brain and pituitary from birth to sexual maturity. 3H-progesterone uptake by various parts of the brain, pituitary and skeletal muscle was compared in newborn, 5-, 10-, 15-, 20-, 25- and 50-day old female rats at 1 hr after a single intraperitoneal injection of 50 muCi/100g body weight. High uptake values in newborn animals and in those aged 5 days were found in all tissues investigated. A sharp decrease in accumulation was observed from birth and/or 5th day of life. The uptake by pituitary was persistantly higher than those by other tissues investigated. The ratio of the concentration of radioactivity between the tissues and the cerebellar cortex increased significantly only in the posterior hypothalamus of adult females (at the age of 50 days). In the pituitary the ratio tissue/cortex was already significantly higher in newborns. The high level of brain radioactivity in the youngest animals is probably a manifestation of high plasma concentrations of tritiated progesterone. The striking decrease in uptake of radioactivity by the brain and pituitary during the first two weeks of life most likely reflects the decrease in level of plasma radioactivity, as shown indirectly by the concomitant decrease in labelled progesterone uptake by the skeletal muscle. The increase in tissue/cortex ratio in the posterior hypothalamus with attainment of sexual maturity suggests the first appearance of specific binding capacity for progesterone which is assumed to be present in the pituitary already from birth."}
{"id": "PMID:1084274", "title": "Morphological changes of thymus and the thyroid gland after postnatal extirpation of pineal body.", "content": "Extirpation of the pineal body of newborn rats was followed by the disorganization of thymic structure, follicular transformation and proliferation, viz. malignant transformation of its epithelial and connective tissue elements. Increase of connective tissue was also observed in the thyroid gland. The follicular cells contained crystal-like bodies and vesicles, whereas in the parafollicular cells the endoplasmic reticulum was increased and light granules appeared.", "contents": "Morphological changes of thymus and the thyroid gland after postnatal extirpation of pineal body. Extirpation of the pineal body of newborn rats was followed by the disorganization of thymic structure, follicular transformation and proliferation, viz. malignant transformation of its epithelial and connective tissue elements. Increase of connective tissue was also observed in the thyroid gland. The follicular cells contained crystal-like bodies and vesicles, whereas in the parafollicular cells the endoplasmic reticulum was increased and light granules appeared."}
{"id": "PMID:1084275", "title": "Changes of glucose metabolism after thyroidectomy in chequered water snake (Natrix piscator).", "content": "Thyroidectomy reduced the rate of removal of glucose from the blood of the chequered water snake (Natrix piscator) maintained at 40 +/- 2.0 degrees C.", "contents": "Changes of glucose metabolism after thyroidectomy in chequered water snake (Natrix piscator). Thyroidectomy reduced the rate of removal of glucose from the blood of the chequered water snake (Natrix piscator) maintained at 40 +/- 2.0 degrees C."}
{"id": "PMID:1084276", "title": "Melatonin in rat pineal, plasma, and urine: 24-hour rhythmicity and effect of chlorpromazine.", "content": "The concentrations of melatonin in rat pineals and in samples of rat plasma and urine were measured by bioassay or radioimmunoassay. Melatonin excretion during the daily dark period (0.98 +/- 0.07 ng/12 h, by bioassay; 1.40 +/- 0.08 ng/12 h, by radioimmunoassay) was much greater than during the light period (0.18 +/- 0.04 ng/12 h, by bioassay; 0.40 +/- 0.04 ng/12 h by radioimmunoassay). (The radioimmunoassay is not completely specific when applied to materials extracted from utine.) Pineal glands and plasmas obtained from animals killed during the daily dark period also contained much more melatonin than samples from animals killed during the light period. Pretreatment with chlorpromazine slowed the disappearance of exogenous melatonin from rat plasma and markedly raised the concentrations of endogenous melatonin in both the pineal gland and the plasma.", "contents": "Melatonin in rat pineal, plasma, and urine: 24-hour rhythmicity and effect of chlorpromazine. The concentrations of melatonin in rat pineals and in samples of rat plasma and urine were measured by bioassay or radioimmunoassay. Melatonin excretion during the daily dark period (0.98 +/- 0.07 ng/12 h, by bioassay; 1.40 +/- 0.08 ng/12 h, by radioimmunoassay) was much greater than during the light period (0.18 +/- 0.04 ng/12 h, by bioassay; 0.40 +/- 0.04 ng/12 h by radioimmunoassay). (The radioimmunoassay is not completely specific when applied to materials extracted from utine.) Pineal glands and plasmas obtained from animals killed during the daily dark period also contained much more melatonin than samples from animals killed during the light period. Pretreatment with chlorpromazine slowed the disappearance of exogenous melatonin from rat plasma and markedly raised the concentrations of endogenous melatonin in both the pineal gland and the plasma."}
{"id": "PMID:1084277", "title": "Movement of histones in chromatin induced by shearing.", "content": "Methylation of accessible DNA within chromatin by restriction modification methylases from Haemophilus influenzae was used to detect movement of histones along the DNA strand during chromatin manipulation. Methylation at different stages of chromatin preparation was followed by titration of the nucleoprotein with ploy(D-lysine), digestion of chromosomal proteins with pronase and analysis of the DNA-poly(D-lysine) complex in steep cesium chloride gradients. Comparison of the specific radioactivities in the peak fractions of the free DNA and the DNA-poly(D-lysine) complex, respectively, reveals that lateral movement of histones, relative to specific sites in the DNA marked by restriction methylases, occurs during manipulation and fragmentation of chromatin.", "contents": "Movement of histones in chromatin induced by shearing. Methylation of accessible DNA within chromatin by restriction modification methylases from Haemophilus influenzae was used to detect movement of histones along the DNA strand during chromatin manipulation. Methylation at different stages of chromatin preparation was followed by titration of the nucleoprotein with ploy(D-lysine), digestion of chromosomal proteins with pronase and analysis of the DNA-poly(D-lysine) complex in steep cesium chloride gradients. Comparison of the specific radioactivities in the peak fractions of the free DNA and the DNA-poly(D-lysine) complex, respectively, reveals that lateral movement of histones, relative to specific sites in the DNA marked by restriction methylases, occurs during manipulation and fragmentation of chromatin."}
{"id": "PMID:1084278", "title": "Phytochrome-mediated assembly of polyribosomes in etiolated bean leaves. Evidence for post-transciptional regulation of development.", "content": "Light operating through phytochrome controls the proportion of total ribosomes present as polyribosomes in etiolated leaves of Phaseolus vulgaris. Similar responses but with slightly different time courses are elicited by brief red light treatment and by continuous far-red or white light. The increase in polyribosome proportions after red light treatment reaches a maximum within 2 h, after which the proportion steadily declines. Light treatment appears to lead to increased proportions of polyribosomes in higher size classes. This is most evident with continuous white light, but probably also occurs with red and far-red light. The increase in polyribosomes is due principally to cytoplasmic ribosomes although proportionately greater effects are observed in chloroplast ribosomes. Although cordycepin inhibits RNA synthesis by 85-90% within 3 h there is no depression of the light-mediated increase in polyribosome proportions when leaves are preincubated in the inhibitor for 3 h. The data therefore indicate that phytochrome is capable of controlling protein synthesis, and thus development, at a post-transciptional level.", "contents": "Phytochrome-mediated assembly of polyribosomes in etiolated bean leaves. Evidence for post-transciptional regulation of development. Light operating through phytochrome controls the proportion of total ribosomes present as polyribosomes in etiolated leaves of Phaseolus vulgaris. Similar responses but with slightly different time courses are elicited by brief red light treatment and by continuous far-red or white light. The increase in polyribosome proportions after red light treatment reaches a maximum within 2 h, after which the proportion steadily declines. Light treatment appears to lead to increased proportions of polyribosomes in higher size classes. This is most evident with continuous white light, but probably also occurs with red and far-red light. The increase in polyribosomes is due principally to cytoplasmic ribosomes although proportionately greater effects are observed in chloroplast ribosomes. Although cordycepin inhibits RNA synthesis by 85-90% within 3 h there is no depression of the light-mediated increase in polyribosome proportions when leaves are preincubated in the inhibitor for 3 h. The data therefore indicate that phytochrome is capable of controlling protein synthesis, and thus development, at a post-transciptional level."}
{"id": "PMID:1084279", "title": "[The influence of bilirubin on the mechanogram of isolated, spontaneously beating frog heart].", "content": "It was shown that bilirubin in its unconjugated form does not only harm the central nervous system. Clinical observations indicate that the toxic effect under certain conditions affects also the heart. In a simple experimental model the influence of free bilirubin on the activity of the heart was investigated. In 69 adult frogs the heart was isolated and exposed sequentially to solutions of different bilirubin concentrations while the heart actions were registered. There was no significant change in the activity of the heart under the influence of bilirubin although concentrations were reached which according to the results of other authors harm cell cultures or evoke in the newborn disturbances in various organs. As a possible explanation, the nonsusceptibility of the heart muscle to bilirubin, the short duration of exposure, and the different behavior of newborn and adult organs is discussed.", "contents": "[The influence of bilirubin on the mechanogram of isolated, spontaneously beating frog heart]. It was shown that bilirubin in its unconjugated form does not only harm the central nervous system. Clinical observations indicate that the toxic effect under certain conditions affects also the heart. In a simple experimental model the influence of free bilirubin on the activity of the heart was investigated. In 69 adult frogs the heart was isolated and exposed sequentially to solutions of different bilirubin concentrations while the heart actions were registered. There was no significant change in the activity of the heart under the influence of bilirubin although concentrations were reached which according to the results of other authors harm cell cultures or evoke in the newborn disturbances in various organs. As a possible explanation, the nonsusceptibility of the heart muscle to bilirubin, the short duration of exposure, and the different behavior of newborn and adult organs is discussed."}
{"id": "PMID:1084284", "title": "[Thrombolytic and pharmacodynamic properties of Aspergillus ochraceus protease].", "content": "The protease isolated from Aspergillus ochraceus exerts a strong thrombolytic action. In in vitro experiments and in tests with animals (mice, rats, dogs) it caused lysis of fibrinous clots. The thrombolytic effect of the protease is conditioned by its direct influence on the fibrin. Besides the thrombolytic action the protease, used within a definite ranges of doses, displays antithrombotic properties. The paper cites data on the acute toxicity (in mice, rats and rabbits) and on the influence of the protease on isolated organs and on the organism of the animals.", "contents": "[Thrombolytic and pharmacodynamic properties of Aspergillus ochraceus protease]. The protease isolated from Aspergillus ochraceus exerts a strong thrombolytic action. In in vitro experiments and in tests with animals (mice, rats, dogs) it caused lysis of fibrinous clots. The thrombolytic effect of the protease is conditioned by its direct influence on the fibrin. Besides the thrombolytic action the protease, used within a definite ranges of doses, displays antithrombotic properties. The paper cites data on the acute toxicity (in mice, rats and rabbits) and on the influence of the protease on isolated organs and on the organism of the animals."}
{"id": "PMID:1084283", "title": "[Participation of the serotonin-reactive brain structure in certain forms of behavior in golden hamsters].", "content": "A viviacious play of young hamsters is shown to be accompanied by a drop of the serotonin level in the brain stem and the subsequent slumber - by its rise, while the corticosteroids content of the peripheral blood with the playful  behavior experiences no changes. Iprazid and 5-oxytryptophan inhibit the playful activity, while dioxyphenylalanina (DOPA) does not influence it. A similar depression of the serotonin level in the brain stem was also noted in an aggressive behavior and stress conditions arising when adult male-hamsters are grouped together. A conclusion is drawn to the effect that changes in the content of serotonin in the brain stem are not associated with the emotional colouration of the condition, but rather reflect the transition from the somnolence to a highly active behavior.", "contents": "[Participation of the serotonin-reactive brain structure in certain forms of behavior in golden hamsters]. A viviacious play of young hamsters is shown to be accompanied by a drop of the serotonin level in the brain stem and the subsequent slumber - by its rise, while the corticosteroids content of the peripheral blood with the playful  behavior experiences no changes. Iprazid and 5-oxytryptophan inhibit the playful activity, while dioxyphenylalanina (DOPA) does not influence it. A similar depression of the serotonin level in the brain stem was also noted in an aggressive behavior and stress conditions arising when adult male-hamsters are grouped together. A conclusion is drawn to the effect that changes in the content of serotonin in the brain stem are not associated with the emotional colouration of the condition, but rather reflect the transition from the somnolence to a highly active behavior."}
{"id": "PMID:1084285", "title": "[Mathematical analysis of the n-cholinolytic effects in experiments with the isolated frog rectus abdominis muscle].", "content": "An inquiry into the nature of antagonism of alpha-tubocurarine and gentamycin to acetylcholine based on a mathematical analysis of cumulative curves obtained in experiments with an isolated musculus rectus abdominalis of the frog showed that the character of changes in the parameters of cumulative curves occuring under the effect of alpha-tubocurarine cannot be explained from the standpoint of Clark's (1929) \"employment theory\", but accords with deductions drawn from a more general theory of allosteric interaction of substances and receptors (Karlin--1967) in competitive antagonism. Variations in the parameters of cumulation curves occurring under the action of gentamycin may be regarded as a manifestation of non-competative to acetylcholine in conformity with both theories.", "contents": "[Mathematical analysis of the n-cholinolytic effects in experiments with the isolated frog rectus abdominis muscle]. An inquiry into the nature of antagonism of alpha-tubocurarine and gentamycin to acetylcholine based on a mathematical analysis of cumulative curves obtained in experiments with an isolated musculus rectus abdominalis of the frog showed that the character of changes in the parameters of cumulative curves occuring under the effect of alpha-tubocurarine cannot be explained from the standpoint of Clark's (1929) \"employment theory\", but accords with deductions drawn from a more general theory of allosteric interaction of substances and receptors (Karlin--1967) in competitive antagonism. Variations in the parameters of cumulation curves occurring under the action of gentamycin may be regarded as a manifestation of non-competative to acetylcholine in conformity with both theories."}
{"id": "PMID:1084286", "title": "[Vestibular tests in studying the spectrum of action of psychotropic preparations].", "content": "As demonstrated by tests set up on rabbits, substances endowed with sedative properties empede habituation to an inadequate stimulation of the vestibular receptors, whereas stimulators facilitate it. When used in small doses imipramin acts as a psycho-stimulator and in large ones--as a tranquilizor.", "contents": "[Vestibular tests in studying the spectrum of action of psychotropic preparations]. As demonstrated by tests set up on rabbits, substances endowed with sedative properties empede habituation to an inadequate stimulation of the vestibular receptors, whereas stimulators facilitate it. When used in small doses imipramin acts as a psycho-stimulator and in large ones--as a tranquilizor."}
{"id": "PMID:1084287", "title": "[Potentiation of the action of reserpine on the frog as a characteristic effect of antidepressants].", "content": "Antidepressants-thymoanaleptics chlorimipramine, amitriptyline, imipramine, melitracen potenciated the inhibitory effect of reserpine in a frog (supression of the turning over from the back reflex) stronger than did antidepressants with less marked thymoanaleptic action (Lu-5-003, azaphen, quipazine, desipramine) and also neuroleptics (promazine, stelazine, haloperidol) cholinolytics (atropine and benectyzine) and stimulator--phenamine. The potentiating action of tertiary amines--imipramine, amitriptyline protixen was stronger than that of the corresponding secondary amines (desipramine, norprotixen, nortriptyline). Phenothiazines with the chlorine atom in the second position (chlorprotixen and chlorpromazine) potentiated the effect of reserpine less intensively than did their chlorine-free analogues (protixen, promazine).", "contents": "[Potentiation of the action of reserpine on the frog as a characteristic effect of antidepressants]. Antidepressants-thymoanaleptics chlorimipramine, amitriptyline, imipramine, melitracen potenciated the inhibitory effect of reserpine in a frog (supression of the turning over from the back reflex) stronger than did antidepressants with less marked thymoanaleptic action (Lu-5-003, azaphen, quipazine, desipramine) and also neuroleptics (promazine, stelazine, haloperidol) cholinolytics (atropine and benectyzine) and stimulator--phenamine. The potentiating action of tertiary amines--imipramine, amitriptyline protixen was stronger than that of the corresponding secondary amines (desipramine, norprotixen, nortriptyline). Phenothiazines with the chlorine atom in the second position (chlorprotixen and chlorpromazine) potentiated the effect of reserpine less intensively than did their chlorine-free analogues (protixen, promazine)."}
{"id": "PMID:1084292", "title": "The role of the macrophage in genetic control of the immune response.", "content": "The ability of an animal to mount an immune response is controlled by a number of autosomal dominant immune response (Ir) genes that are linked to the major histocompatibility complex of the species. In the guinea pig, alloantiserums raised by cross-immunization of inbred strain 2 and strain 13 animals specifically inhibited the in vitro proliferative responses of (2 X 13) F1 lymphocytes to those antigens the response to which is controlled by Ir genes linked to the alloantigens against which the serums are directed. A genetic analysis indicated that the inhibitory activity of the alloantiserums was directed against the alloantigens rather than the products of specific Ir genes. The interaction of antigen-pulsed macrophages with immune T lymphocytes is also mediated by the 2/13 alloantigens and alloantiserums are capable of inhibiting macrophage-T lymphocyte interaction. Studies involving combinations of macrophages and lymphocytes that differed at alloantigens or Ir gene products or both raised the possibility of the expression of the Ir gene product in the macrophage.", "contents": "The role of the macrophage in genetic control of the immune response. The ability of an animal to mount an immune response is controlled by a number of autosomal dominant immune response (Ir) genes that are linked to the major histocompatibility complex of the species. In the guinea pig, alloantiserums raised by cross-immunization of inbred strain 2 and strain 13 animals specifically inhibited the in vitro proliferative responses of (2 X 13) F1 lymphocytes to those antigens the response to which is controlled by Ir genes linked to the alloantigens against which the serums are directed. A genetic analysis indicated that the inhibitory activity of the alloantiserums was directed against the alloantigens rather than the products of specific Ir genes. The interaction of antigen-pulsed macrophages with immune T lymphocytes is also mediated by the 2/13 alloantigens and alloantiserums are capable of inhibiting macrophage-T lymphocyte interaction. Studies involving combinations of macrophages and lymphocytes that differed at alloantigens or Ir gene products or both raised the possibility of the expression of the Ir gene product in the macrophage."}
{"id": "PMID:1084293", "title": "The role of suppressor cells in the pathogenesis of common variable hypogammaglobulinemia and the immunodeficiency associated with myeloma.", "content": "The role of suppressor cells in the pathogenesis of immunodeficiency was analyzed using a technique that permits study of the differentiation of B lymphocytes into immunoglobulin-synthesizing plasma cells. Lymphocytes from normals synthesized 4,910 ng of IgM, 1,270 ng of IgA, and 1,625 ng of IgG per 2 X 10(6) cells when cultured for 7 days in the presence of pokeweed mitogen. In contrast the lymphocytes from patients with common variable hypogammaglobulinemia did not synthesize significant quantities of immunoglobulin. When lymphocytes from 9 of 13 patients with common variable hypogammaglobulinemia studied were cocultured with normal lymphocytes, the synthesis of immunoglobulin by the normal lymphocytes was depressed by 75-100%. A comparable suppression of immunoglobulin synthesis by normal lymphocytes was observed when they were cocultured with T cells from hypogammaglobulinemic patients. These studies suggest that in some patients the disease common variable hypogammaglobulinemia may not be due to an intrinsic defect of B cells alone but may be cuased or perpetuated by an abnormality of regulatory T cells that act to suppress B-cell maturation and antibody production. Peripheral blood lymphocytes from myeloma patients also had a drastically reduced capacity to produce polyclonal immunoglobulins. Three of 6 myeloma patients tested had circulating mononuclear cells that suppressed immunoglobulin production by cocultured normal lymphocytes. Purified T cells from myeloma patients did not mediate this suppressor effect. These observations suggest that one mechanism for the humoral immune deficiency observed in myeloma patients is a block of polyclonal B-cell maturation by suppressor cells.", "contents": "The role of suppressor cells in the pathogenesis of common variable hypogammaglobulinemia and the immunodeficiency associated with myeloma. The role of suppressor cells in the pathogenesis of immunodeficiency was analyzed using a technique that permits study of the differentiation of B lymphocytes into immunoglobulin-synthesizing plasma cells. Lymphocytes from normals synthesized 4,910 ng of IgM, 1,270 ng of IgA, and 1,625 ng of IgG per 2 X 10(6) cells when cultured for 7 days in the presence of pokeweed mitogen. In contrast the lymphocytes from patients with common variable hypogammaglobulinemia did not synthesize significant quantities of immunoglobulin. When lymphocytes from 9 of 13 patients with common variable hypogammaglobulinemia studied were cocultured with normal lymphocytes, the synthesis of immunoglobulin by the normal lymphocytes was depressed by 75-100%. A comparable suppression of immunoglobulin synthesis by normal lymphocytes was observed when they were cocultured with T cells from hypogammaglobulinemic patients. These studies suggest that in some patients the disease common variable hypogammaglobulinemia may not be due to an intrinsic defect of B cells alone but may be cuased or perpetuated by an abnormality of regulatory T cells that act to suppress B-cell maturation and antibody production. Peripheral blood lymphocytes from myeloma patients also had a drastically reduced capacity to produce polyclonal immunoglobulins. Three of 6 myeloma patients tested had circulating mononuclear cells that suppressed immunoglobulin production by cocultured normal lymphocytes. Purified T cells from myeloma patients did not mediate this suppressor effect. These observations suggest that one mechanism for the humoral immune deficiency observed in myeloma patients is a block of polyclonal B-cell maturation by suppressor cells."}
{"id": "PMID:1084294", "title": "Effect of serum proteinase inhibitors on the fertilizing capacity of rabbit spermatozoa.", "content": "The in vivo effect of purified human alpha1-antitrypsin and inter-alpha-trypsin inhibitor on the fertilizing ability of capacitated rabbit spermatozoa was investigated and compared with that of the trypsin-kallikrein inhibitor from bovine organs (Trasylol). Only 250 mug of alpha1-antitrypsin/5 X 10(4) sperm in 0.05 ml inhibited fertilization by more than 50%. Lower concentrations of alpha1-antitrypsin (50 mug/5 X 10(4) sperm in 0.05 mo) and inter-alpha-trypsin inhibitor in amounts of 250 mug/5 X 10(4) sperm and less had no significant effect on the fertilization rate. Comparative experiments with Trasylol in conentrations of 50 mug/5 X 10(4) sperm resulted in more than 50% fertilization inhibition, whereas 100 mug/5 X 10(4) sperm decreased the fertilization rate by approximately 90%. The differences in the antifertility effect of these acrosin inhibitors toward capacitated spermatozoa may be due to differences in molecular weight. The effective concentrations required for fertilization inhibition appear to be relatively high under the experimental conditions used.", "contents": "Effect of serum proteinase inhibitors on the fertilizing capacity of rabbit spermatozoa. The in vivo effect of purified human alpha1-antitrypsin and inter-alpha-trypsin inhibitor on the fertilizing ability of capacitated rabbit spermatozoa was investigated and compared with that of the trypsin-kallikrein inhibitor from bovine organs (Trasylol). Only 250 mug of alpha1-antitrypsin/5 X 10(4) sperm in 0.05 ml inhibited fertilization by more than 50%. Lower concentrations of alpha1-antitrypsin (50 mug/5 X 10(4) sperm in 0.05 mo) and inter-alpha-trypsin inhibitor in amounts of 250 mug/5 X 10(4) sperm and less had no significant effect on the fertilization rate. Comparative experiments with Trasylol in conentrations of 50 mug/5 X 10(4) sperm resulted in more than 50% fertilization inhibition, whereas 100 mug/5 X 10(4) sperm decreased the fertilization rate by approximately 90%. The differences in the antifertility effect of these acrosin inhibitors toward capacitated spermatozoa may be due to differences in molecular weight. The effective concentrations required for fertilization inhibition appear to be relatively high under the experimental conditions used."}
{"id": "PMID:1084296", "title": "[The mechanism of development of changes in the structure and histochemistry of a neuron].", "content": "The change of the neuron's functional state when the cells become unable to generate spike responses to orthodromic testing stimuli, was shown not to be reflected in the morphological pattern of motoneurons as revealed by means of Einarsson's RNA staining. High-frequency stimulation of the motoneurons generating neither postsynaptic nor spike potentials diminishes the neurons size and intensifies their staining. A strong activation of presynaptic endings followed by no active postsynaptic processes on the neuron's membrane was shown to be able to entail major morphological alterations.", "contents": "[The mechanism of development of changes in the structure and histochemistry of a neuron]. The change of the neuron's functional state when the cells become unable to generate spike responses to orthodromic testing stimuli, was shown not to be reflected in the morphological pattern of motoneurons as revealed by means of Einarsson's RNA staining. High-frequency stimulation of the motoneurons generating neither postsynaptic nor spike potentials diminishes the neurons size and intensifies their staining. A strong activation of presynaptic endings followed by no active postsynaptic processes on the neuron's membrane was shown to be able to entail major morphological alterations."}
{"id": "PMID:1084297", "title": "[Passive and active elements of the potassium secretion system in the skin of frogs].", "content": "A method has been evolved for studying the potassium and sodium transport without labelled ions in the frog skin. The potassium secretion sharply increased after the nystatinum had been added to the solution near the skin surface. Suppression of the apical membrane sodium permeability with amiloride or triamteren did not alter the potassium secretion velocity. The nystatinum increased both the potassium and sodium permeability. The blocking agents for sodium channels became ineffective in the presence of nystatinum. During the nystatinum action on the apical membrane the work of Na/K pump does not change in the basal plasmatic membrane, while suppression of the pump with strophantinum abolishes the potassium secretion across the apical membrane. Vasopressin and aldosterone increase the potassium secretion. Therefore, the potassium secretion level depends on the apical membrane potassium permeability and is independent of the sodium transport across this membrane, it is due to potassium inflow into the cell with the aid of Na/K pump of the basal plasmatic membrane.", "contents": "[Passive and active elements of the potassium secretion system in the skin of frogs]. A method has been evolved for studying the potassium and sodium transport without labelled ions in the frog skin. The potassium secretion sharply increased after the nystatinum had been added to the solution near the skin surface. Suppression of the apical membrane sodium permeability with amiloride or triamteren did not alter the potassium secretion velocity. The nystatinum increased both the potassium and sodium permeability. The blocking agents for sodium channels became ineffective in the presence of nystatinum. During the nystatinum action on the apical membrane the work of Na/K pump does not change in the basal plasmatic membrane, while suppression of the pump with strophantinum abolishes the potassium secretion across the apical membrane. Vasopressin and aldosterone increase the potassium secretion. Therefore, the potassium secretion level depends on the apical membrane potassium permeability and is independent of the sodium transport across this membrane, it is due to potassium inflow into the cell with the aid of Na/K pump of the basal plasmatic membrane."}
{"id": "PMID:1084299", "title": "[Responses of the neuromuscular synapses of chronically desympathized frog muscles to tetanic nerve stimulation].", "content": "7--14 days after unilateral section of the abdominal sympathetic trunk, the quantum content of the end plate potential (e.p.p.) in the m. sartorius of the frog decreased. The facilitation and fluctuations of the e.p.p. amplitude during train of stimuli (20, 50, 100/sec) became more obvious. Noradrenaline (5-10(-6) g/ml) increased the amplitude of a single e.p.p. and the facilitation during repetitive stimulation. The effect of noradrenaline in sympathectomized muscles was more obvious than in the control muscles. After the noradrenaline administration, the fourfold increase of calcium concentration did not change the amplitude of e.p.p.s or the time course of the facilitation. The mechanism of depolarization--secretion coupling in the nerve terminal seems to be impaired by the sympathectomy.", "contents": "[Responses of the neuromuscular synapses of chronically desympathized frog muscles to tetanic nerve stimulation]. 7--14 days after unilateral section of the abdominal sympathetic trunk, the quantum content of the end plate potential (e.p.p.) in the m. sartorius of the frog decreased. The facilitation and fluctuations of the e.p.p. amplitude during train of stimuli (20, 50, 100/sec) became more obvious. Noradrenaline (5-10(-6) g/ml) increased the amplitude of a single e.p.p. and the facilitation during repetitive stimulation. The effect of noradrenaline in sympathectomized muscles was more obvious than in the control muscles. After the noradrenaline administration, the fourfold increase of calcium concentration did not change the amplitude of e.p.p.s or the time course of the facilitation. The mechanism of depolarization--secretion coupling in the nerve terminal seems to be impaired by the sympathectomy."}
{"id": "PMID:1084300", "title": "[The effect of cholinesterase inhibitors on the synaptic potentials of frog neuromuscular junctions].", "content": "Prostigmine and the organophosphorus inhibitor armine acting upon synaptic potentials in the frog cutaneous pectori nerve--muscle preparation, increased the m.e.p.p. and e.p.p. amplitudes, lengthened the rise and the half--decline time of the e.p.p. in a-proximately similar way. The effect of armine developed faster than that of prostigmine. The presynaptic effects of both anticholinesterases (increase of mepp's frequency and quantal content of epp's) developed much more slowly than their post-synaptic effects. After prostigmine pretreatment during 2 hrs, addition of armine caused lengthening of the time-course of e.p.p.'s. but the presynaptic effect was not altered. After armine pretreatment, prostigmine did not cause any further effect. Possible mechanisms for the presynaptic effects of the inhibitors are discussed.", "contents": "[The effect of cholinesterase inhibitors on the synaptic potentials of frog neuromuscular junctions]. Prostigmine and the organophosphorus inhibitor armine acting upon synaptic potentials in the frog cutaneous pectori nerve--muscle preparation, increased the m.e.p.p. and e.p.p. amplitudes, lengthened the rise and the half--decline time of the e.p.p. in a-proximately similar way. The effect of armine developed faster than that of prostigmine. The presynaptic effects of both anticholinesterases (increase of mepp's frequency and quantal content of epp's) developed much more slowly than their post-synaptic effects. After prostigmine pretreatment during 2 hrs, addition of armine caused lengthening of the time-course of e.p.p.'s. but the presynaptic effect was not altered. After armine pretreatment, prostigmine did not cause any further effect. Possible mechanisms for the presynaptic effects of the inhibitors are discussed."}
{"id": "PMID:1084301", "title": "[Morpho-physiologic characteristics of the fiber spectrum of a frog external eye muscle].", "content": "By means of histochemical and microelectrophysiological methods, fibers of the eye inferior rectus muscle of the frog were divided into types with phasic, tonic (two types), and transitional (two types) signs. Electric activity of the muscle in situ reveals presence of phasic, transitional, and tonic fibers in the given object (the tonic fibers being characterized by a wide range of the PSP parameters).", "contents": "[Morpho-physiologic characteristics of the fiber spectrum of a frog external eye muscle]. By means of histochemical and microelectrophysiological methods, fibers of the eye inferior rectus muscle of the frog were divided into types with phasic, tonic (two types), and transitional (two types) signs. Electric activity of the muscle in situ reveals presence of phasic, transitional, and tonic fibers in the given object (the tonic fibers being characterized by a wide range of the PSP parameters)."}
{"id": "PMID:1084303", "title": "[The influence of synaptic processes on changes in neuronal structure].", "content": "Reactions of the spinal motoneurons to the afferent inflow were different in frogs: some neurons generated spike potentials during 10-min rhythmic stimulation (1/sec) of afferents, other neurons were switched on and off. The motoneurons and their synapses increased in size, their cytoplasmic matrix became lighter and ribosoms deteriorated. Antidromic activation did not entail modofocation of size or color of the cells. RNP granules were safe and local organellas degenerated. The role of synaptic processes in regulation of RNP metabolism and in intracellular reparation, was revealed.", "contents": "[The influence of synaptic processes on changes in neuronal structure]. Reactions of the spinal motoneurons to the afferent inflow were different in frogs: some neurons generated spike potentials during 10-min rhythmic stimulation (1/sec) of afferents, other neurons were switched on and off. The motoneurons and their synapses increased in size, their cytoplasmic matrix became lighter and ribosoms deteriorated. Antidromic activation did not entail modofocation of size or color of the cells. RNP granules were safe and local organellas degenerated. The role of synaptic processes in regulation of RNP metabolism and in intracellular reparation, was revealed."}
{"id": "PMID:1084306", "title": "Retinopathy in Pima Indians. Relationships to glucose level, duration of diabetes, age at diagnosis of diabetes, and age at examination in a population with a high prevalence of diabetes mellitus.", "content": "The occurrence of retinopathy and its relationship to diabetes in 1,640 Pima Indians age 15 and over has been determined. Eighteen per cent of those with two-hour postload plasma glucose levels of equal to or greater than 200 mg./dl. had some evidence of retinopathy. Of those with retinopathy and diabetes, 7 per cent were found to have proliferative or neovascular changes, the remainder having microaneurysms and/or exudates. The frequency of retinopathy increased from 3 per cent among newly diagnosed diabetics to 47 per cent among those with diabetes of 10 or more years duration. No relationship was found with sex, age at diagnosis of diabetes, or age at time of examination when duration of diabetes was taken into account. The occurrence of retinopathy was confined largely to those who fell into the second or hyperglycemic component of the frequency distribution of plasma glucose levels in the population, indicating the significance of the bimodal glucose tolerance frequency distribution.", "contents": "Retinopathy in Pima Indians. Relationships to glucose level, duration of diabetes, age at diagnosis of diabetes, and age at examination in a population with a high prevalence of diabetes mellitus. The occurrence of retinopathy and its relationship to diabetes in 1,640 Pima Indians age 15 and over has been determined. Eighteen per cent of those with two-hour postload plasma glucose levels of equal to or greater than 200 mg./dl. had some evidence of retinopathy. Of those with retinopathy and diabetes, 7 per cent were found to have proliferative or neovascular changes, the remainder having microaneurysms and/or exudates. The frequency of retinopathy increased from 3 per cent among newly diagnosed diabetics to 47 per cent among those with diabetes of 10 or more years duration. No relationship was found with sex, age at diagnosis of diabetes, or age at time of examination when duration of diabetes was taken into account. The occurrence of retinopathy was confined largely to those who fell into the second or hyperglycemic component of the frequency distribution of plasma glucose levels in the population, indicating the significance of the bimodal glucose tolerance frequency distribution."}
{"id": "PMID:1084307", "title": "Coronary heart disease in the Pima Indians. Electrocardiographic findings and postmortem evidence of myocardial infarction in a population with a high prevalence of diabetes mellitus.", "content": "The frequency of electrocardiographic evidence of coronary heart disease (CHD) and the rate of autopsy-proved myocardial infarction were determined in the Pima, a tribe of American Indians with a high prevalence of diabetes mellitus. The electrocardiograms of 701 Pimas, aged 40 years and over (85 per cent of the adult reservation population, 45 per cent of whom had diabetes) were read according to the Minnesota Code, and 120 postmortem examinations were reviewed for evidence of myocardial infarction. The frequency of CHD as evidenced by major Q-wave changes in the Pima (1.6/100) was about one-half that found in Tecumseh, Michigan (p less than 0.10). The relatively low rate of myocardial infarction at autopsy (15 per cent of males and 8 per cent of females aged 40 years and over) was consistent with the low prevalence of Q-wave changes. The subjects with diabetes had a higher rate of CHD than nondiabetics, both electrocardiographically and at postmortem examination, although the differences were not statistically significant. The low prevalence of CHD in the living Pima and the low rate of infarction at autopsy indicate that this tribe has a low frequency of CHD despite the extraordinarily high prevalence of diabetes mellitus.", "contents": "Coronary heart disease in the Pima Indians. Electrocardiographic findings and postmortem evidence of myocardial infarction in a population with a high prevalence of diabetes mellitus. The frequency of electrocardiographic evidence of coronary heart disease (CHD) and the rate of autopsy-proved myocardial infarction were determined in the Pima, a tribe of American Indians with a high prevalence of diabetes mellitus. The electrocardiograms of 701 Pimas, aged 40 years and over (85 per cent of the adult reservation population, 45 per cent of whom had diabetes) were read according to the Minnesota Code, and 120 postmortem examinations were reviewed for evidence of myocardial infarction. The frequency of CHD as evidenced by major Q-wave changes in the Pima (1.6/100) was about one-half that found in Tecumseh, Michigan (p less than 0.10). The relatively low rate of myocardial infarction at autopsy (15 per cent of males and 8 per cent of females aged 40 years and over) was consistent with the low prevalence of Q-wave changes. The subjects with diabetes had a higher rate of CHD than nondiabetics, both electrocardiographically and at postmortem examination, although the differences were not statistically significant. The low prevalence of CHD in the living Pima and the low rate of infarction at autopsy indicate that this tribe has a low frequency of CHD despite the extraordinarily high prevalence of diabetes mellitus."}
{"id": "PMID:1084308", "title": "Diabetes mellitus: incidence, prevalence, survivorship, and causes of death in Rochester, Minnesota, 1945-1970.", "content": "The incidence and prevalence of diabetes mellitus in residents of Rochester, Minnesota, for 25 years (1945 to 1970) were determined from available medical records. The over-all incidence rate for diabetes is 133 new cases per 100,000 population per year (age-adjusted to 1970 U.S. white population). The rate increased with age for both men and women and was higher among men over 30 years of age. The average annual incidence rates per five-year period for juvenile-onset diabetes mellitus were low and variable and showed little change. Polyuria, polydipsia, glycosuria, lean habitus, loss of weight, and high levels of fasting hyperglycemia at initial diagnosis occurred more frequently in younger than in older patients. The peak incidence in 1960 through 1964 and the decrease in the following five years may be a reflection of the introduction of the AutoAnalyzer method for blood glucose in 1958. The average annual incidence rates for 1955 through 1959 and 1965 through 1969 were essentially the same. The over-all prevalence for diabetes mellitus is 1.6 per cent, with a higher rate among men than among women over 40 years of age; among school children the rate is 0.1 per cent. Survivorship in the diabetic population is lower than that in the general population. The leading cause of death was coronary heart disease, the death rate from it being higher than for the general population.", "contents": "Diabetes mellitus: incidence, prevalence, survivorship, and causes of death in Rochester, Minnesota, 1945-1970. The incidence and prevalence of diabetes mellitus in residents of Rochester, Minnesota, for 25 years (1945 to 1970) were determined from available medical records. The over-all incidence rate for diabetes is 133 new cases per 100,000 population per year (age-adjusted to 1970 U.S. white population). The rate increased with age for both men and women and was higher among men over 30 years of age. The average annual incidence rates per five-year period for juvenile-onset diabetes mellitus were low and variable and showed little change. Polyuria, polydipsia, glycosuria, lean habitus, loss of weight, and high levels of fasting hyperglycemia at initial diagnosis occurred more frequently in younger than in older patients. The peak incidence in 1960 through 1964 and the decrease in the following five years may be a reflection of the introduction of the AutoAnalyzer method for blood glucose in 1958. The average annual incidence rates for 1955 through 1959 and 1965 through 1969 were essentially the same. The over-all prevalence for diabetes mellitus is 1.6 per cent, with a higher rate among men than among women over 40 years of age; among school children the rate is 0.1 per cent. Survivorship in the diabetic population is lower than that in the general population. The leading cause of death was coronary heart disease, the death rate from it being higher than for the general population."}
{"id": "PMID:1084309", "title": "The immune response in cirrhotic rats. The induction of tolerance to orally administered protein antigens.", "content": "Hepatic cirrhosis reduced the susceptibility of rats to the induction of tolerance by the oral administration of a protein antigen. Rats with portacaval shunt were rendered tolerant as readily as normal rats. The orally induced state of partial tolerance was shown to be dependent on thymus-dependent lymphocytes: B lymphocytes reacted normally to challenge when injected with T lymphocytes from normal rats. Several factors may contribute to the reduced responsiveness of the cirrhotic rats to the tolerance regime. First, the cirrhotic liver was shown to have a reduced capacity to separate immunogen from tolerogen. Second, because of the reduced phagocytic capacity of the liver, increased quantities of lipopolysaccharide, derived from intestinal microorganisms, enter the blood stream. These substances and products of hepatocyte necrosis have adjuvant activity and may therefore contribute to the changed state of responsiveness of rats with cirrhosis.", "contents": "The immune response in cirrhotic rats. The induction of tolerance to orally administered protein antigens. Hepatic cirrhosis reduced the susceptibility of rats to the induction of tolerance by the oral administration of a protein antigen. Rats with portacaval shunt were rendered tolerant as readily as normal rats. The orally induced state of partial tolerance was shown to be dependent on thymus-dependent lymphocytes: B lymphocytes reacted normally to challenge when injected with T lymphocytes from normal rats. Several factors may contribute to the reduced responsiveness of the cirrhotic rats to the tolerance regime. First, the cirrhotic liver was shown to have a reduced capacity to separate immunogen from tolerogen. Second, because of the reduced phagocytic capacity of the liver, increased quantities of lipopolysaccharide, derived from intestinal microorganisms, enter the blood stream. These substances and products of hepatocyte necrosis have adjuvant activity and may therefore contribute to the changed state of responsiveness of rats with cirrhosis."}
{"id": "PMID:1084310", "title": "Fiberoptic endoscopy during laparotomy in the diagnosis of small intestinal bleeding.", "content": "A patient with recurrent gastrointestinal bleeding caused by hereditary hemorrhagic telangiectasia localized to the small intestine is described. The diagnosis was made by combining two recognized investigational tools-fiberoptic endoscopy and laparotomy.", "contents": "Fiberoptic endoscopy during laparotomy in the diagnosis of small intestinal bleeding. A patient with recurrent gastrointestinal bleeding caused by hereditary hemorrhagic telangiectasia localized to the small intestine is described. The diagnosis was made by combining two recognized investigational tools-fiberoptic endoscopy and laparotomy."}
{"id": "PMID:1084311", "title": "Mallory-Weiss syndrome. Characterization of 75 Mallory-weiss lacerations in 528 patients with upper gastrointestinal hemorrhage.", "content": "Seventy-five Mallory-Weiss lacerations were visualized endoscopically in 58 of 528 patients evaluated acutely for upper gastrointestinal bleeding. The Mallory-Weiss mucosal laceration is more common than generally recognized, is usually associated with hiatal hernia and a prodrome of retching or vomiting, and the ingestion of alcohol or acetylsalicyclic acid, or both. The lacerations are most commonly gastric and are associated with other mucosal lesions which may in fact be the instigating cause of the retching and vomiting. Although blood loss may be considerable, 90% or more patients with this lesion can be managed nonsurgically with appropriate blood component replacement and occasional use of systemic pitressin. There was one fatality in this series of 58 patients with Mallory-Weiss lacerations.", "contents": "Mallory-Weiss syndrome. Characterization of 75 Mallory-weiss lacerations in 528 patients with upper gastrointestinal hemorrhage. Seventy-five Mallory-Weiss lacerations were visualized endoscopically in 58 of 528 patients evaluated acutely for upper gastrointestinal bleeding. The Mallory-Weiss mucosal laceration is more common than generally recognized, is usually associated with hiatal hernia and a prodrome of retching or vomiting, and the ingestion of alcohol or acetylsalicyclic acid, or both. The lacerations are most commonly gastric and are associated with other mucosal lesions which may in fact be the instigating cause of the retching and vomiting. Although blood loss may be considerable, 90% or more patients with this lesion can be managed nonsurgically with appropriate blood component replacement and occasional use of systemic pitressin. There was one fatality in this series of 58 patients with Mallory-Weiss lacerations."}
{"id": "PMID:1084313", "title": "Functional T cells in rabbits gut mucosal lymphocytes.", "content": "Functional studies of Peyer's patch and gut mucosa lymphocytes were performed in mixed lymphocyte culture reaction (MLC), an in vitro measure of the capacity of T cells to recognize and respond to cell surface alloantigens. The data demonstrate the functional effectiveness of both these mucosal lymphocyte populations in one-way MLC, both as responding as well as stimulating cells. Both the responding and stimulating capacities of these cells in MLC were almost the same as observed for lymphocytes obtained from either spleen or thymus. We conclude that functional T cells exist in both rabbit gut lamina propria and Peyer's patches with approximately the same quantitative capacity to respond in the MLC.", "contents": "Functional T cells in rabbits gut mucosal lymphocytes. Functional studies of Peyer's patch and gut mucosa lymphocytes were performed in mixed lymphocyte culture reaction (MLC), an in vitro measure of the capacity of T cells to recognize and respond to cell surface alloantigens. The data demonstrate the functional effectiveness of both these mucosal lymphocyte populations in one-way MLC, both as responding as well as stimulating cells. Both the responding and stimulating capacities of these cells in MLC were almost the same as observed for lymphocytes obtained from either spleen or thymus. We conclude that functional T cells exist in both rabbit gut lamina propria and Peyer's patches with approximately the same quantitative capacity to respond in the MLC."}
{"id": "PMID:1084314", "title": "T cell depletion in untreated adult coeliac disease.", "content": "The proportional and absolute numbers of circulating thymus dependent lymphocytes (T cells) were reduced in untreated patients with coeliac disease but were normal after treatment with a gluten free diet. There was an inverse correlation between circulating T cell numbers and jejunal intraepithelial lymphocytes. This evidence suggests a possible role for T cells in the pathogenesis of coeliac disease and is a further example of disturbed cell mediated immunity in this condition.", "contents": "T cell depletion in untreated adult coeliac disease. The proportional and absolute numbers of circulating thymus dependent lymphocytes (T cells) were reduced in untreated patients with coeliac disease but were normal after treatment with a gluten free diet. There was an inverse correlation between circulating T cell numbers and jejunal intraepithelial lymphocytes. This evidence suggests a possible role for T cells in the pathogenesis of coeliac disease and is a further example of disturbed cell mediated immunity in this condition."}
{"id": "PMID:1084318", "title": "[Diverticulosis--diverticulitis].", "content": "Diverticulosis is found in approximately 5% of people living in western industrialized countries. 95% of these are without any symptoms and do not need any therapy. For prophylaxis of diverticulitis a diet containing non-digestable cellulose and swelling substances is recommended. Complications of diverticulosis like diverticulitis, perforation abscess, or stenosis can necessitate a resection of the sigmoid colon. This should be done before life-threatening complications like free perforation or massive bleeding occur.", "contents": "[Diverticulosis--diverticulitis]. Diverticulosis is found in approximately 5% of people living in western industrialized countries. 95% of these are without any symptoms and do not need any therapy. For prophylaxis of diverticulitis a diet containing non-digestable cellulose and swelling substances is recommended. Complications of diverticulosis like diverticulitis, perforation abscess, or stenosis can necessitate a resection of the sigmoid colon. This should be done before life-threatening complications like free perforation or massive bleeding occur."}
{"id": "PMID:1084322", "title": "Lymphocyte trapping. Differential effects of ATS and irradiation on trapping in lymph nodes and spleen.", "content": "Increased sequestration (trapping) of lymphocytes occurs in lymphoid organs following antigenic challenge. The effects of irradiation and anti-thymocyte serum (ATS) upon lymphocyte trapping in lymph nodes and spleen were studied. Both the above agents diminished or abrogated trapping in the draining lymph nodes; these same agents resulted in enhanced trapping in the spleen. Suppression of lymph node trapping could be achieved with a low dose of ATS and with 850 R, but not 200 R. These results are interpreted as showing that lymphocyte trapping in lymph nodes is initiated primarily by an ATS- and irradiation-sensitive population, whereas in the spleen a cell population resistant to the above agents activates the lymphocyte trap. These data demonstrate that the cell populations and/or the mechanisms involved in lymphocyte trapping in lymph nodes and spleens are distinct.", "contents": "Lymphocyte trapping. Differential effects of ATS and irradiation on trapping in lymph nodes and spleen. Increased sequestration (trapping) of lymphocytes occurs in lymphoid organs following antigenic challenge. The effects of irradiation and anti-thymocyte serum (ATS) upon lymphocyte trapping in lymph nodes and spleen were studied. Both the above agents diminished or abrogated trapping in the draining lymph nodes; these same agents resulted in enhanced trapping in the spleen. Suppression of lymph node trapping could be achieved with a low dose of ATS and with 850 R, but not 200 R. These results are interpreted as showing that lymphocyte trapping in lymph nodes is initiated primarily by an ATS- and irradiation-sensitive population, whereas in the spleen a cell population resistant to the above agents activates the lymphocyte trap. These data demonstrate that the cell populations and/or the mechanisms involved in lymphocyte trapping in lymph nodes and spleens are distinct."}
{"id": "PMID:1084327", "title": "Isolated mesenteric injury due to blunt abdominal trauma.", "content": "Isolated injuries of the small bowel mesentery or mesocolon with subsequent bowel infarction due to blunt abdominal trauma are rare. Two cases are described: 1 involving the mesentery to the terminal ileum and 1 involving the transverse mesocolon and middle colic artery, both with bowel infarction. The modes of clinical presentation and management of patients with injuries to the mesentery, mesocolon and mesenteric vessels following blunt trauma are described.", "contents": "Isolated mesenteric injury due to blunt abdominal trauma. Isolated injuries of the small bowel mesentery or mesocolon with subsequent bowel infarction due to blunt abdominal trauma are rare. Two cases are described: 1 involving the mesentery to the terminal ileum and 1 involving the transverse mesocolon and middle colic artery, both with bowel infarction. The modes of clinical presentation and management of patients with injuries to the mesentery, mesocolon and mesenteric vessels following blunt trauma are described."}
{"id": "PMID:1084328", "title": "Activation of purified human T cells by mitogens: diminished mitogen-induced deoxyribonucleic acid synthesis in human T cells compared with autologous peripheral blood lymphocytes.", "content": "Human thymus-derived (T) cells were isolated from peripheral blood after rosette formation with neuraminidase-treated sheep erythrocytes (SRBC). After separation on Ficoll-hypaque, SRBC were removed from T cells by treatment with tris(hydroxymethyl)aminomethane-NH4Cl. Human T cells and autologous peripheral blood lymphocytes (PBL) were then incubated with phytohemagglutinin, concanavalin A, or pokeweed mitogen. Human T cells, in the absence of other cell types, responded with less deoxyribonucleic acid (DNA) synthesis (measured by uptake of [3H]thymidine) than equal numbers of autologous PBL. Further experimentation established that, compared with autologous PBL, the diminished capacity of human T cells to be activated by mitogens was due neither to differences in the mitogen dose-response relationship nor to the time of peak DNA synthesis of T cells or autologous PBL. Fragments or components of SRBC were not detected on human T cells, and treatment of the T cell-SRBC mixture with tris(hydroxymethyl)aminomethane-NH4Cl did not contribute to the results. Increased cell density or a period of preculture before addition of mitogen also did not influence the degree of decreased DNA synthesis in human T cells compared with the response of autologous PBL incubated with the same mitogens. When mixed with the cells remaining at the Ficoll-media interface, purified human T cells did not suppress the mitogenic response of this cell mixture to PHA or concanavalin A. The data indicated that human T cells, in the absence of other cell types, were activated by mitogens to a lesser degree than autologous PBL. Furthermore, T cells responded with DNA synthesis after direct T cell-mitogen interaction.", "contents": "Activation of purified human T cells by mitogens: diminished mitogen-induced deoxyribonucleic acid synthesis in human T cells compared with autologous peripheral blood lymphocytes. Human thymus-derived (T) cells were isolated from peripheral blood after rosette formation with neuraminidase-treated sheep erythrocytes (SRBC). After separation on Ficoll-hypaque, SRBC were removed from T cells by treatment with tris(hydroxymethyl)aminomethane-NH4Cl. Human T cells and autologous peripheral blood lymphocytes (PBL) were then incubated with phytohemagglutinin, concanavalin A, or pokeweed mitogen. Human T cells, in the absence of other cell types, responded with less deoxyribonucleic acid (DNA) synthesis (measured by uptake of [3H]thymidine) than equal numbers of autologous PBL. Further experimentation established that, compared with autologous PBL, the diminished capacity of human T cells to be activated by mitogens was due neither to differences in the mitogen dose-response relationship nor to the time of peak DNA synthesis of T cells or autologous PBL. Fragments or components of SRBC were not detected on human T cells, and treatment of the T cell-SRBC mixture with tris(hydroxymethyl)aminomethane-NH4Cl did not contribute to the results. Increased cell density or a period of preculture before addition of mitogen also did not influence the degree of decreased DNA synthesis in human T cells compared with the response of autologous PBL incubated with the same mitogens. When mixed with the cells remaining at the Ficoll-media interface, purified human T cells did not suppress the mitogenic response of this cell mixture to PHA or concanavalin A. The data indicated that human T cells, in the absence of other cell types, were activated by mitogens to a lesser degree than autologous PBL. Furthermore, T cells responded with DNA synthesis after direct T cell-mitogen interaction."}
{"id": "PMID:1084329", "title": "Suppressive effect of a chronic helminth infection, schistosomiasis mansoni, on the in vitro responses of spleen and lymph node cells to the T cell mitogens phytohemagglutinin and concanavalin A.", "content": "Chronic murine schistosomiasis mansoni is associated with depressed cell-mediated immune responses to Schistosoma mansoni egg antigens. The present study has examined the possibility that factors develop during infection that are capable of altering the response of lymphocytes to stimuli other than specific schistosomal antigens. Egg production begins at 5 weeks, and 1 to 3 weeks later there is a moderate degree of unresponsiveness of lymph node and spleen cells to the mitogens concanavalin A and phytohemagglutinin. This was associated with an altered dose response curve to the mitogens similar to that observed in antigenic systems. Seven weeks after the initiation of antigenic stimulation (egg prodiction lymph node and spleen cells from chronically infected animals were profoundly unresponsive to all concentrations of concanavalin A and phytohemagglutinin tested. These investigations suggest that, in addition to possible blockade by serum antibody, other suppressive factors may be involved in the spontaneous modulation of immunopathology in chronic schistosomiasis. These are detectable 1 to 3 weeks after the onset of egg production and are prominent at 12 weeks. Such findings are consistent with, but do not prove, the existence of suppressor T cells in chronic schistosomiasis.", "contents": "Suppressive effect of a chronic helminth infection, schistosomiasis mansoni, on the in vitro responses of spleen and lymph node cells to the T cell mitogens phytohemagglutinin and concanavalin A. Chronic murine schistosomiasis mansoni is associated with depressed cell-mediated immune responses to Schistosoma mansoni egg antigens. The present study has examined the possibility that factors develop during infection that are capable of altering the response of lymphocytes to stimuli other than specific schistosomal antigens. Egg production begins at 5 weeks, and 1 to 3 weeks later there is a moderate degree of unresponsiveness of lymph node and spleen cells to the mitogens concanavalin A and phytohemagglutinin. This was associated with an altered dose response curve to the mitogens similar to that observed in antigenic systems. Seven weeks after the initiation of antigenic stimulation (egg prodiction lymph node and spleen cells from chronically infected animals were profoundly unresponsive to all concentrations of concanavalin A and phytohemagglutinin tested. These investigations suggest that, in addition to possible blockade by serum antibody, other suppressive factors may be involved in the spontaneous modulation of immunopathology in chronic schistosomiasis. These are detectable 1 to 3 weeks after the onset of egg production and are prominent at 12 weeks. Such findings are consistent with, but do not prove, the existence of suppressor T cells in chronic schistosomiasis."}
{"id": "PMID:1084330", "title": "T-dependence of human B lymphocyte proliferative response to mitogens.", "content": "Human peripheral blood and tonsil lymphocytes were fractionated on anti-Ig-coated Sephadex columns or by centrifugation after rosetting with native sheep erythrocytes. Both methods allowed the recovery of B and T-enriched populations the purity of which was checked by fluorescein-labelled anti-Ig serum, E and EAC rosette formation, and heterologous antisera specific for B or T lymphocytes. The proliferative response of T cells to PHA, Con A, PWM, and ALS was not found different from that of unfractionated cells, whereas no response of the B cells could be observed to these mitogens providing that no contaminating T cells were present. Addition of T lymphocytes to these unresponsive B cells allowed them to respond to phytomitogens, but not to ALS. X-irradiated T cells could, to some extent, replace the diving T lymphocytes; no T-replacing factor could be found in cell-free supernatants from T cells, whether or not they had been activated by mitrogens. This model of B-T cooperation appears useful for studying the differentiation and maturation of human B lymphocytes.", "contents": "T-dependence of human B lymphocyte proliferative response to mitogens. Human peripheral blood and tonsil lymphocytes were fractionated on anti-Ig-coated Sephadex columns or by centrifugation after rosetting with native sheep erythrocytes. Both methods allowed the recovery of B and T-enriched populations the purity of which was checked by fluorescein-labelled anti-Ig serum, E and EAC rosette formation, and heterologous antisera specific for B or T lymphocytes. The proliferative response of T cells to PHA, Con A, PWM, and ALS was not found different from that of unfractionated cells, whereas no response of the B cells could be observed to these mitogens providing that no contaminating T cells were present. Addition of T lymphocytes to these unresponsive B cells allowed them to respond to phytomitogens, but not to ALS. X-irradiated T cells could, to some extent, replace the diving T lymphocytes; no T-replacing factor could be found in cell-free supernatants from T cells, whether or not they had been activated by mitrogens. This model of B-T cooperation appears useful for studying the differentiation and maturation of human B lymphocytes."}
{"id": "PMID:1084331", "title": "Differences between lymphoid cell populations of guinea pigs and mice as determined by the response to mitogens in vitro.", "content": "The stimulation of guinea pig lymphocytes by phytohemagglutinin (PHA), concanavalin A (ConA), methanol-extracted residues of tubercle bacilli (MER), purified protein derivative of tubercle bacilli (PPD), dextran sulphate (DS) and E. coli lipopolysaccharide (LPS), was determined and compared with that of mouse lymphoid cells. The sources of lymphocytes tested were spleen, thymus, lymph nodes and bone marrow. The degree of activation of DNA synthesis by PHA and ConA was higher in guinea pig thymocytes and lymph node cells than in corresponding sources of mouse lymphocytes. The optimum degree of stimulation by PHA and ConA was approximately the same in guinea pig thymocytes, while ConA was by far a better stimulator than PHA for mouse thymocytes. All four B-cell mitogens tested (MER, DS, PPD and LPS) activated DNA synthesis in mouse lymphoid cells while only MER and DS were effective in guinea pig lymphocytes. A guinea pig spleen cell population depleted from B cells was not stimulated, neither by DS nor by MER, while it still responded to PHA and ConA. These results indicate that the proliferative response due to MER and DS occurs in the B-cell compartment. It is suggested that the differences between guinea pigs and mice with respect to their ability to develop a cell-mediated type immunity and to respond to T-independent antigens are related to differences in the relative proportions and degrees of maturation of T- and B-cell subpopulations, as reflected by the selective responsiveness to various mitogens.", "contents": "Differences between lymphoid cell populations of guinea pigs and mice as determined by the response to mitogens in vitro. The stimulation of guinea pig lymphocytes by phytohemagglutinin (PHA), concanavalin A (ConA), methanol-extracted residues of tubercle bacilli (MER), purified protein derivative of tubercle bacilli (PPD), dextran sulphate (DS) and E. coli lipopolysaccharide (LPS), was determined and compared with that of mouse lymphoid cells. The sources of lymphocytes tested were spleen, thymus, lymph nodes and bone marrow. The degree of activation of DNA synthesis by PHA and ConA was higher in guinea pig thymocytes and lymph node cells than in corresponding sources of mouse lymphocytes. The optimum degree of stimulation by PHA and ConA was approximately the same in guinea pig thymocytes, while ConA was by far a better stimulator than PHA for mouse thymocytes. All four B-cell mitogens tested (MER, DS, PPD and LPS) activated DNA synthesis in mouse lymphoid cells while only MER and DS were effective in guinea pig lymphocytes. A guinea pig spleen cell population depleted from B cells was not stimulated, neither by DS nor by MER, while it still responded to PHA and ConA. These results indicate that the proliferative response due to MER and DS occurs in the B-cell compartment. It is suggested that the differences between guinea pigs and mice with respect to their ability to develop a cell-mediated type immunity and to respond to T-independent antigens are related to differences in the relative proportions and degrees of maturation of T- and B-cell subpopulations, as reflected by the selective responsiveness to various mitogens."}
{"id": "PMID:1084332", "title": "Passive sensitization of tissue cells. V. The detection of chicken antibodies cytophilic for basophils and eosinophils.", "content": "Basophil-cytophilic antibodies were domonstrated in sera of chickens injected with either sheep erythrocytes or avian tuberculin. Normal chicken basophils were passively sensitized in vitro, and two types of cytophilic antibodies were shown by rosette-formation: (a) heat-stable, mercaptoethanol-resistant antibodies, and (b) heat-labile, mercaptoethanol-sensitive antibodies. Serum from one of the chickens injected with sheep erythrocytes also passively sensitized normal, non-basophilic granulocytes which would appear from stained smears to be eosinophils. Cytophilic activity in the serum correlated with the rosette-forming reactions of circulating eosinophils and/or basophils from each of the sensitized donor birds.", "contents": "Passive sensitization of tissue cells. V. The detection of chicken antibodies cytophilic for basophils and eosinophils. Basophil-cytophilic antibodies were domonstrated in sera of chickens injected with either sheep erythrocytes or avian tuberculin. Normal chicken basophils were passively sensitized in vitro, and two types of cytophilic antibodies were shown by rosette-formation: (a) heat-stable, mercaptoethanol-resistant antibodies, and (b) heat-labile, mercaptoethanol-sensitive antibodies. Serum from one of the chickens injected with sheep erythrocytes also passively sensitized normal, non-basophilic granulocytes which would appear from stained smears to be eosinophils. Cytophilic activity in the serum correlated with the rosette-forming reactions of circulating eosinophils and/or basophils from each of the sensitized donor birds."}
{"id": "PMID:1084333", "title": "Antibodies to nucleic acids in myasthenia gravis.", "content": "An increased frequency of antibodies to native DNA, thymocytes, and striated muscle was found in patients with myasthenia gravis (MG). The prevalence of such antibodies lends considerable support to the concept of MG as an autoimmune disorder and militates in favor of major abnormalities in the thymic dependent immune system. There was no correlation between serum-blocking activity to acetylcholine receptor protein and antibodies to thymocytes.", "contents": "Antibodies to nucleic acids in myasthenia gravis. An increased frequency of antibodies to native DNA, thymocytes, and striated muscle was found in patients with myasthenia gravis (MG). The prevalence of such antibodies lends considerable support to the concept of MG as an autoimmune disorder and militates in favor of major abnormalities in the thymic dependent immune system. There was no correlation between serum-blocking activity to acetylcholine receptor protein and antibodies to thymocytes."}
{"id": "PMID:1084336", "title": "An acquired color defect of the opponent-color system.", "content": "An acquired unilateral color defect in a 22-year-old man has been investigated with standard clinical tests and by using techniques which, it is thought, test specifically for the sensitivity of the luminance and opponent-color systems. The spectral sensitivity of the defective left eye, using 1 degree 200 ms. test flashes on a white background, has a single broad peak at about 550 nm. and resembles the photopic luminosity curve; in contrast, the normal curve, measured in the same conditions, has three peaks at about 440, 520, and 600 nm. However, the subject's spectral sensitivity curve for detecting 20 Hz. flicker is quite normal and is similar to his curve for 200 ms. flashes. It has recently been proposed that the three peaks of the normal curve for 200 ms. flashes reflect the activity of the opponent-color system, whereas the single peak for flicker detection is related to the luminance system. The preceding observations may thus be interpreted in terms of a specific loss of the subject's opponent-color system and this would explain his poor color discrimination. His luminance system appears to be normal, and evidence is presented for the maintained function of red- and green-sensitive (but not blue-sensitive) cones. The spectral sensitivity of the subject's right eye is nearly normal, suggesting a precortical origin of the defect; however, there seems to be some abnormality in this eye, indicating a less developed form of the same defect.", "contents": "An acquired color defect of the opponent-color system. An acquired unilateral color defect in a 22-year-old man has been investigated with standard clinical tests and by using techniques which, it is thought, test specifically for the sensitivity of the luminance and opponent-color systems. The spectral sensitivity of the defective left eye, using 1 degree 200 ms. test flashes on a white background, has a single broad peak at about 550 nm. and resembles the photopic luminosity curve; in contrast, the normal curve, measured in the same conditions, has three peaks at about 440, 520, and 600 nm. However, the subject's spectral sensitivity curve for detecting 20 Hz. flicker is quite normal and is similar to his curve for 200 ms. flashes. It has recently been proposed that the three peaks of the normal curve for 200 ms. flashes reflect the activity of the opponent-color system, whereas the single peak for flicker detection is related to the luminance system. The preceding observations may thus be interpreted in terms of a specific loss of the subject's opponent-color system and this would explain his poor color discrimination. His luminance system appears to be normal, and evidence is presented for the maintained function of red- and green-sensitive (but not blue-sensitive) cones. The spectral sensitivity of the subject's right eye is nearly normal, suggesting a precortical origin of the defect; however, there seems to be some abnormality in this eye, indicating a less developed form of the same defect."}
{"id": "PMID:1084338", "title": "An intact globe technique for electrophysiological studies on cornea.", "content": "An intact globe method was developed to determine the characteristics of the cornea of the bullfrog, Rana catesbeiana. With this method the anterior chamber could be perfused and the transcorneal potential difference (PD) and electrical resistance determined. It was found for the endothelium plus stroma (epithelium scraped) that the PD was essentially zero and the electrical resistance was only a small fraction of that of the intact cornea. Elevation of K+ or decrease in Cl- concentration in the anterior chamber produced in intact corneas a large and rapid change in PD while with the epithelium scraped (stroma and endothelium intact) these elevations produced a negligible change in PD. It is concluded that ions can rapidly move across the endothelium and stroma of the cornea.", "contents": "An intact globe technique for electrophysiological studies on cornea. An intact globe method was developed to determine the characteristics of the cornea of the bullfrog, Rana catesbeiana. With this method the anterior chamber could be perfused and the transcorneal potential difference (PD) and electrical resistance determined. It was found for the endothelium plus stroma (epithelium scraped) that the PD was essentially zero and the electrical resistance was only a small fraction of that of the intact cornea. Elevation of K+ or decrease in Cl- concentration in the anterior chamber produced in intact corneas a large and rapid change in PD while with the epithelium scraped (stroma and endothelium intact) these elevations produced a negligible change in PD. It is concluded that ions can rapidly move across the endothelium and stroma of the cornea."}
{"id": "PMID:1084339", "title": "Similarity in properties and mapping of three Rec mutants of Haemophilus influenzae.", "content": "Three Rec- mutants of Haemophilus influenzae have been studied with respect to their transformability, ultraviolet and mitomycin C sensitivities, spontaneous and ultraviolet-induced deoxyribonucleic acid breakdown, inducibility of lysogens, and the linkage of the three mutations to a streptomycin resistance marker. The data indicate that the three mutations cause the same phenotypic changes, and that they are all on the same gene. Transformability of the mutants is different when two different media are used for competence development, although transformability with the two competence methods is not different in a Rec- strain that is mutant at another gene.", "contents": "Similarity in properties and mapping of three Rec mutants of Haemophilus influenzae. Three Rec- mutants of Haemophilus influenzae have been studied with respect to their transformability, ultraviolet and mitomycin C sensitivities, spontaneous and ultraviolet-induced deoxyribonucleic acid breakdown, inducibility of lysogens, and the linkage of the three mutations to a streptomycin resistance marker. The data indicate that the three mutations cause the same phenotypic changes, and that they are all on the same gene. Transformability of the mutants is different when two different media are used for competence development, although transformability with the two competence methods is not different in a Rec- strain that is mutant at another gene."}
{"id": "PMID:1084340", "title": "Ultrastructural characterization of capsulated Haemophilus influenzae type b and two spontaneous nontypable mutants.", "content": "Capsulated Haemophilus influenzae type b and two spontaneous mutants (classes I and II variants) were characterized by transmission and scanning electron microscopy. When cells were treated with type b-specific antiserum prior to manipulations for electron microscopy, sectioned capsulated cells had electron-dense, fibrous capsular antigen-antibody complexes around them. In negatively stained preparations, the complexes appeared as electron-transparent zones surrounding cells. In contrast, only residual electron-dense, extracellular material was seen in sectioned, untreated, capsulated cells, and electron-dense \"bridges\" connected adjacent cells in negatively stained preparations. No extracellular capsular material was seen around the class I and II variants. Characteristic electron-translucent regions were always observed within the cytosol of the class I cells, both in thin sections and by negative staining. These areas were located adjacent to the cell envelope separating the plasma membrane from the dense cytoplasmic matrix. At times, electron-dense, thread-like material extended from the dense cytoplasmic matrix to the plasma membrane. No such regions were seen in the capsulated and class II cells. Class I cells fixed with methanol or suspended in NaCl or phosphate-buffered saline prior to treatment with fluorescein-tagged type b-specific antiserum (FTA reagent) exhibited, by immunofluorescence, patches of capsular antigen along their sides. However, when fixed with glutaraldehyde or OsO4 or suspended in tris-(hydroxymethyl)aminomethane plus Ca2+ buffer prior to treatment with FTA reagent, no patches of capsular antigen were seen. Subsequent exposure of the latter cells to methanol followed by treatment with FTA reagent resulted in the reappearance of the patches of capsular antigen. Thus, in the class I variant the capsular antigen is unlikely to be surface located. Scanning electron microscopy revealed that class I and II variant cells within undisturbed colonies were regularly aligned side-by-side, whereas cells within colonies of the capsulated strain were randomly distributed.", "contents": "Ultrastructural characterization of capsulated Haemophilus influenzae type b and two spontaneous nontypable mutants. Capsulated Haemophilus influenzae type b and two spontaneous mutants (classes I and II variants) were characterized by transmission and scanning electron microscopy. When cells were treated with type b-specific antiserum prior to manipulations for electron microscopy, sectioned capsulated cells had electron-dense, fibrous capsular antigen-antibody complexes around them. In negatively stained preparations, the complexes appeared as electron-transparent zones surrounding cells. In contrast, only residual electron-dense, extracellular material was seen in sectioned, untreated, capsulated cells, and electron-dense \"bridges\" connected adjacent cells in negatively stained preparations. No extracellular capsular material was seen around the class I and II variants. Characteristic electron-translucent regions were always observed within the cytosol of the class I cells, both in thin sections and by negative staining. These areas were located adjacent to the cell envelope separating the plasma membrane from the dense cytoplasmic matrix. At times, electron-dense, thread-like material extended from the dense cytoplasmic matrix to the plasma membrane. No such regions were seen in the capsulated and class II cells. Class I cells fixed with methanol or suspended in NaCl or phosphate-buffered saline prior to treatment with fluorescein-tagged type b-specific antiserum (FTA reagent) exhibited, by immunofluorescence, patches of capsular antigen along their sides. However, when fixed with glutaraldehyde or OsO4 or suspended in tris-(hydroxymethyl)aminomethane plus Ca2+ buffer prior to treatment with FTA reagent, no patches of capsular antigen were seen. Subsequent exposure of the latter cells to methanol followed by treatment with FTA reagent resulted in the reappearance of the patches of capsular antigen. Thus, in the class I variant the capsular antigen is unlikely to be surface located. Scanning electron microscopy revealed that class I and II variant cells within undisturbed colonies were regularly aligned side-by-side, whereas cells within colonies of the capsulated strain were randomly distributed."}
{"id": "PMID:1084341", "title": "Synthesis of envelope polypeptides by Haemophilus influenzae during development of competence for genetic transformation.", "content": "Six polypeptides with apparent molecular weights of 95,000, 90,000, 80,000, 67,000, 64,000, and 43,000 were found to be characteristic of the cell envelopes of competent Haemophilus influenzae, and were synthesized entirely during the period of competence development. Two polypeptides with apparent molecular weights of 58,500 and 40,500 were synthesized during growth as well as during competence development, but were only associated with the envelope fraction of cells that had developed competence. The kinetics of synthesis of the competence-related envelope polypeptides showed a lag period of approximately 20 min. The observation of this lag period raises the question as to whether some of these competence-related polypeptides might be involved in the process of deoxyribonucleic acid uptake, since the development of this property also exhibits a sigmoid time course during competence development.", "contents": "Synthesis of envelope polypeptides by Haemophilus influenzae during development of competence for genetic transformation. Six polypeptides with apparent molecular weights of 95,000, 90,000, 80,000, 67,000, 64,000, and 43,000 were found to be characteristic of the cell envelopes of competent Haemophilus influenzae, and were synthesized entirely during the period of competence development. Two polypeptides with apparent molecular weights of 58,500 and 40,500 were synthesized during growth as well as during competence development, but were only associated with the envelope fraction of cells that had developed competence. The kinetics of synthesis of the competence-related envelope polypeptides showed a lag period of approximately 20 min. The observation of this lag period raises the question as to whether some of these competence-related polypeptides might be involved in the process of deoxyribonucleic acid uptake, since the development of this property also exhibits a sigmoid time course during competence development."}
{"id": "PMID:1084344", "title": "The mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. The source of the third methyl hydrogen in the 5'-deoxyadenosine generated from the cofactor during catalysis.", "content": "Ethanolamine ammonia-lyase is an adenosylcobalamin-dependent enzyme which catalyzes the conversion of ethanolamine and propanolamine to ammonia and the corresponding aldehydes. A mechanism has been proposed for this and other adenosylcobalamin-dependent reactions which involves cleavage of the carbon-cobalt bond of the cofactor followed by abstraction of a substrate hydrogen atom by the adenosyl fragment to form 5'-deoxyadenosine. In support of this proposal, a previous study demonstrated that the deamination of propanolamine by ethanolamine ammonia-lyase is accompanied by the reversible cleavage of the carbon-cobalt bond of the cofactor, with the production of 5'-deoxyadenosine (Babior, B.M., Carty, T.J., and Abeles, R.H. (1974) J. Biol. Chem. 249, 1689-1695). The present study is concerned with the origin of the third hydrogen atom on the methyl group of the 5'-deoxyadenosine produced in that reaction. The 5'-deoxyadenosine isolated from an incubation mixture initially containing enzyme, [5',5'-D2]adenosylcobalamin, and [1,1-D2]propanolamine was chemically degraded so that the 4' and 5' carbon atoms were, respectively, converted to the carbonyl and methyl carbons of acetaldehyde. Analysis of the p-nitrophenylhydrazone of the acetaldehyde by gas-liquid chromatography-mass spectroscopy revealed 3 deuterium atoms/molecule, indicating that two of the methyl hydrogens originated from adenosylcobalamin and the third was donated by substrate. This observation provides further support for the participation of 5'-deoxyadenosine in the mechanism of adenosylcobalamin-dependent reactions.", "contents": "The mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. The source of the third methyl hydrogen in the 5'-deoxyadenosine generated from the cofactor during catalysis. Ethanolamine ammonia-lyase is an adenosylcobalamin-dependent enzyme which catalyzes the conversion of ethanolamine and propanolamine to ammonia and the corresponding aldehydes. A mechanism has been proposed for this and other adenosylcobalamin-dependent reactions which involves cleavage of the carbon-cobalt bond of the cofactor followed by abstraction of a substrate hydrogen atom by the adenosyl fragment to form 5'-deoxyadenosine. In support of this proposal, a previous study demonstrated that the deamination of propanolamine by ethanolamine ammonia-lyase is accompanied by the reversible cleavage of the carbon-cobalt bond of the cofactor, with the production of 5'-deoxyadenosine (Babior, B.M., Carty, T.J., and Abeles, R.H. (1974) J. Biol. Chem. 249, 1689-1695). The present study is concerned with the origin of the third hydrogen atom on the methyl group of the 5'-deoxyadenosine produced in that reaction. The 5'-deoxyadenosine isolated from an incubation mixture initially containing enzyme, [5',5'-D2]adenosylcobalamin, and [1,1-D2]propanolamine was chemically degraded so that the 4' and 5' carbon atoms were, respectively, converted to the carbonyl and methyl carbons of acetaldehyde. Analysis of the p-nitrophenylhydrazone of the acetaldehyde by gas-liquid chromatography-mass spectroscopy revealed 3 deuterium atoms/molecule, indicating that two of the methyl hydrogens originated from adenosylcobalamin and the third was donated by substrate. This observation provides further support for the participation of 5'-deoxyadenosine in the mechanism of adenosylcobalamin-dependent reactions."}
{"id": "PMID:1084345", "title": "DNA synthesis and turnover in the bullfrog tadpole during metamorphosis.", "content": "125I-labeled deoxyuridine (IdUrd) has been used to estimate the turnover of DNA in liver, tail, and hind limb during spontaneous and triiodothyronine-induced metamorphosis. It was found that the total amount of liver DNA remained constant and there was no significant loss of the label from the liver DNA, which would be expected if there was an increase in DNA turnover during metamorphosis. Also, the change in specific activity of liver DNA parallels that of tail DNA during spontaneous metamorphosis. These data suggest that metamorphic transitions in the tadpole liver do not involve significant changes in DNA turnover. It was observed that the incorporation of label into hind limb DNA showed a high variability among individual animals as compared to liver and tail tissue. The data presented suggest that the observed variability is not a random phenomenon but related directly to the rate at which animals will metamorphose.", "contents": "DNA synthesis and turnover in the bullfrog tadpole during metamorphosis. 125I-labeled deoxyuridine (IdUrd) has been used to estimate the turnover of DNA in liver, tail, and hind limb during spontaneous and triiodothyronine-induced metamorphosis. It was found that the total amount of liver DNA remained constant and there was no significant loss of the label from the liver DNA, which would be expected if there was an increase in DNA turnover during metamorphosis. Also, the change in specific activity of liver DNA parallels that of tail DNA during spontaneous metamorphosis. These data suggest that metamorphic transitions in the tadpole liver do not involve significant changes in DNA turnover. It was observed that the incorporation of label into hind limb DNA showed a high variability among individual animals as compared to liver and tail tissue. The data presented suggest that the observed variability is not a random phenomenon but related directly to the rate at which animals will metamorphose."}
{"id": "PMID:1084348", "title": "Multidrug chemotherapy in pulmonary treatment of osteosarcoma.", "content": "Forty-three patients with osteosarcoma were treated with amputation and adjuvant chemotherapy utilizing a four-drug combination of cyclophosphamide, vincristine, phenylalanine mustard, and adriamycin (CONPADRI-I regimen). Twenty-four patients (56 per cent) remained free of metastases twelve to sixty-one months after diagnosis. Ten of the twenty-four have been disease-free for more than three years. Another group of thirty patients was treated with amputation and a five-drug adjuvant chemotherapy program which included the administration of massive doses of methotrexate with citrovorum factor (COMPADRI-II regimen). Twenty of the thirty (67 per cent) remained free of metastases from twelve to twenty-six months after amputation (median, sixteen months). Two deaths related to methotrexate toxicity occurred. Late metastases developed in three patients (at sixteen, nineteen, and twenty-six months after operation) in the group treated with the COMPADRI-II regimen.", "contents": "Multidrug chemotherapy in pulmonary treatment of osteosarcoma. Forty-three patients with osteosarcoma were treated with amputation and adjuvant chemotherapy utilizing a four-drug combination of cyclophosphamide, vincristine, phenylalanine mustard, and adriamycin (CONPADRI-I regimen). Twenty-four patients (56 per cent) remained free of metastases twelve to sixty-one months after diagnosis. Ten of the twenty-four have been disease-free for more than three years. Another group of thirty patients was treated with amputation and a five-drug adjuvant chemotherapy program which included the administration of massive doses of methotrexate with citrovorum factor (COMPADRI-II regimen). Twenty of the thirty (67 per cent) remained free of metastases from twelve to twenty-six months after amputation (median, sixteen months). Two deaths related to methotrexate toxicity occurred. Late metastases developed in three patients (at sixteen, nineteen, and twenty-six months after operation) in the group treated with the COMPADRI-II regimen."}
{"id": "PMID:1084349", "title": "BCG osteomyelitis: experience in the Stockholm region over the years 1961-1974.", "content": "Eighteen cases of bone and joint tuberculosis in children were diagnosed in the Stockholm region (about 1,500,000 population) over the period 1961-1974. BCG infection was verified by culture and identification of bacterial type in seven, all after 1968. The same origin can be presumed in most of the remaining eleven cases, in spite of the absence of bacterial verification. The increased frequency of complications after BCG vaccination may necessitate a revision of the vaccination programme. We recommend operative treatment, which has not led to any growth disturbances or impairment of joint function, although the lesions were invariably localised close to growth zones and joints.", "contents": "BCG osteomyelitis: experience in the Stockholm region over the years 1961-1974. Eighteen cases of bone and joint tuberculosis in children were diagnosed in the Stockholm region (about 1,500,000 population) over the period 1961-1974. BCG infection was verified by culture and identification of bacterial type in seven, all after 1968. The same origin can be presumed in most of the remaining eleven cases, in spite of the absence of bacterial verification. The increased frequency of complications after BCG vaccination may necessitate a revision of the vaccination programme. We recommend operative treatment, which has not led to any growth disturbances or impairment of joint function, although the lesions were invariably localised close to growth zones and joints."}
{"id": "PMID:1084350", "title": "Synaptic activity of frog retinal photoreceptors. A peroxidase uptake study.", "content": "The uptake of horseradish peroxidase (HRP) into membranous structures, detectable by light and electron microscopy, is used here to monitor the synaptic activity of photoreceptors of isolated frog retinas maintained in the dark or under various illumination conditions. The major findings are: (a) Neurotransmission from photoreceptor terminals seems to involve the same types of endocytic membrane-retrieval processes that occur at other nerve terminals. Presumably, the endocytic processes compensate for exocytic events associated with neurotransmission. The retrieved membrane is \"recycled\" to form vesicles. Some of these accumulate near the synaptic ribbons, perhaps indicating reutilization for exocytosis. On the other hand, some retrieved membrane evidently is degraded via multivesicular bodies that appear to undergo \"retrograde\" transport from the receptor synapses to the myoid regions. (b) Photoreceptor terminals take up much HRP in the dark. Steady illumination markedly decreases uptake by rods. Uptake by cones is notably reduced only at illumination intensities higher than those that have maximal effects on rods. (c) The decrease in rod HRP uptake with light is reversible when retinas are allowed to adapt to the dark, if the light exposures used were at intensities that bleach very little visual pigment. Such \"recovery\" is not observed after light exposures that bleach a considerable amount of visual pigment. The cones recover their dark levels of HRP uptake even after light exposures that bleach considerable amounts of visual pigment. The changes in HRP uptake that we observe parallel expectations for photoreceptor synaptic neurotransmission derived from indirect physiological evidence.", "contents": "Synaptic activity of frog retinal photoreceptors. A peroxidase uptake study. The uptake of horseradish peroxidase (HRP) into membranous structures, detectable by light and electron microscopy, is used here to monitor the synaptic activity of photoreceptors of isolated frog retinas maintained in the dark or under various illumination conditions. The major findings are: (a) Neurotransmission from photoreceptor terminals seems to involve the same types of endocytic membrane-retrieval processes that occur at other nerve terminals. Presumably, the endocytic processes compensate for exocytic events associated with neurotransmission. The retrieved membrane is \"recycled\" to form vesicles. Some of these accumulate near the synaptic ribbons, perhaps indicating reutilization for exocytosis. On the other hand, some retrieved membrane evidently is degraded via multivesicular bodies that appear to undergo \"retrograde\" transport from the receptor synapses to the myoid regions. (b) Photoreceptor terminals take up much HRP in the dark. Steady illumination markedly decreases uptake by rods. Uptake by cones is notably reduced only at illumination intensities higher than those that have maximal effects on rods. (c) The decrease in rod HRP uptake with light is reversible when retinas are allowed to adapt to the dark, if the light exposures used were at intensities that bleach very little visual pigment. Such \"recovery\" is not observed after light exposures that bleach a considerable amount of visual pigment. The cones recover their dark levels of HRP uptake even after light exposures that bleach considerable amounts of visual pigment. The changes in HRP uptake that we observe parallel expectations for photoreceptor synaptic neurotransmission derived from indirect physiological evidence."}
{"id": "PMID:1084351", "title": "[Intracystic hemorrhages of pancreatic pseudocysts].", "content": "The authors report ten personal cases and review the literature on 99 other cases, and study primary intra-cystic hemorrhage or post-operative hemorrhage, occurring during the course of pancreatic pseudo-cysts. These hemorrhagic pseudo-cysts are very often associated with chronic pancreatitis; they may rupture into a hollow viscus, the peritoneal cavity or into Wirsung's duct. Arteriography and duodenoscopy with retrograde pancreatography, should permit one to reduce the frequency of these latent, undiagnosed forms until surgical operation has been carried out. Even more dangerous, post-operative bleeding often represents a recurrence of primary intra-cystic hemorrhage which has been undiagnosed or neglected. Surgical treatment should give a greater place to operations of excision which simultaneously treat false cyst, the responsible vascular lesion and the chronic casual pancreatitis.", "contents": "[Intracystic hemorrhages of pancreatic pseudocysts]. The authors report ten personal cases and review the literature on 99 other cases, and study primary intra-cystic hemorrhage or post-operative hemorrhage, occurring during the course of pancreatic pseudo-cysts. These hemorrhagic pseudo-cysts are very often associated with chronic pancreatitis; they may rupture into a hollow viscus, the peritoneal cavity or into Wirsung's duct. Arteriography and duodenoscopy with retrograde pancreatography, should permit one to reduce the frequency of these latent, undiagnosed forms until surgical operation has been carried out. Even more dangerous, post-operative bleeding often represents a recurrence of primary intra-cystic hemorrhage which has been undiagnosed or neglected. Surgical treatment should give a greater place to operations of excision which simultaneously treat false cyst, the responsible vascular lesion and the chronic casual pancreatitis."}
{"id": "PMID:1084352", "title": "The effects of epinephrine infusion in patients with von Willebrand's disease.", "content": "Epinephrine infusion causes variable increases in the components of the Factor VIII (antihemophilic factor) complex in patients with von Willebrand's disease. The increase in antihemophilic factor procoagulant activity was greater than that of Factor VIII-related antigen and von Willebrand factor activity in two patients with von Willebrand's disease. Similar increases in the three individual factors were demonstrated in two other patients. A 4-10-fold increase in Factor VIII-related properties was identified in each of these individuals after infusion. One patient has been studied with very severe von Willebrand's disease; none of the Factor VIII-related properties increased despite two infusions of epinephrine. Bleeding times were normalized or remained normal in the two patients whose von Willebrand factor activity was greater than 25 U/100 ml. It remained prolonged in those three patients whose von Willebrand factor activity levels remained below that concentration. The increase in procoagulant activity was transient in all patients and t 1/2 values were estimated to be between 0.8 and 3.4 h.", "contents": "The effects of epinephrine infusion in patients with von Willebrand's disease. Epinephrine infusion causes variable increases in the components of the Factor VIII (antihemophilic factor) complex in patients with von Willebrand's disease. The increase in antihemophilic factor procoagulant activity was greater than that of Factor VIII-related antigen and von Willebrand factor activity in two patients with von Willebrand's disease. Similar increases in the three individual factors were demonstrated in two other patients. A 4-10-fold increase in Factor VIII-related properties was identified in each of these individuals after infusion. One patient has been studied with very severe von Willebrand's disease; none of the Factor VIII-related properties increased despite two infusions of epinephrine. Bleeding times were normalized or remained normal in the two patients whose von Willebrand factor activity was greater than 25 U/100 ml. It remained prolonged in those three patients whose von Willebrand factor activity levels remained below that concentration. The increase in procoagulant activity was transient in all patients and t 1/2 values were estimated to be between 0.8 and 3.4 h."}
{"id": "PMID:1084353", "title": "Suppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients.", "content": "Leukocytes from patients with several forms of immunodeficiency characterized by apparently differing defects in B-lymphocyte maturation produced few or no plasmacytoid cells in vitro, and were capable of suppressing the generation of plasma cells in co-culture with cells of normal persons, in the presence of pokeweed mitogen. Such inhibition was commonly observed in cultures which included cells from patients with primary immunoglobulin deficiency, but was not seen to a significant degree in identical co-cultures of cells from normals. The suppression observed was not dependent upon mixed leukocyte culture reactivity. Both sheep erythrocyte-rosetting lymphocytes and adherent cells appeared to participate in these effects in some patients. In one patient with common variable immunodeficiency, but not in several others, removal of suppressing cells permitted the patient's remaining cells to differentiate into plasma cells in vitro. Because of the diverse syndromes in which suppression was observed, it is likely that, in at least some hypogammaglobulinemic patients, the suppression is secondary to the disease process rather than being the primary pathogenic mechanism.", "contents": "Suppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients. Leukocytes from patients with several forms of immunodeficiency characterized by apparently differing defects in B-lymphocyte maturation produced few or no plasmacytoid cells in vitro, and were capable of suppressing the generation of plasma cells in co-culture with cells of normal persons, in the presence of pokeweed mitogen. Such inhibition was commonly observed in cultures which included cells from patients with primary immunoglobulin deficiency, but was not seen to a significant degree in identical co-cultures of cells from normals. The suppression observed was not dependent upon mixed leukocyte culture reactivity. Both sheep erythrocyte-rosetting lymphocytes and adherent cells appeared to participate in these effects in some patients. In one patient with common variable immunodeficiency, but not in several others, removal of suppressing cells permitted the patient's remaining cells to differentiate into plasma cells in vitro. Because of the diverse syndromes in which suppression was observed, it is likely that, in at least some hypogammaglobulinemic patients, the suppression is secondary to the disease process rather than being the primary pathogenic mechanism."}
{"id": "PMID:1084354", "title": "Heterogeneity of lymphocyte subpopulations in severe combined immunodeficiency. Evidence against a stem cell defect.", "content": "Surface markers typical of T and B lymphocytes were present on varying proportions of peripheral blood lymphocytes from three infants with severe combined immunodeficiency disease. Despite this, functions mediated by T and B cells were either absent or very minimal in all three, including cell-mediated responses in vivo; the in vitro proliferative response to mitogens, allogeneic cells, or antigens; effector cell function in lymphocyte-antibody lymphocytolytic interaction assays; and in vitro synthesis of IgG, IgA, and IgM. In contrast, mononuclear cells from one of the infants were tested and found capable of lysing both human and chicken antibody-coated erythrocyte targets normally. Co-cultivation experiments with unrelated normal control lymphocytes failed to demonstrate suppressor cell activity for immunoglobulin synthesis in these infants. Augmentations of immunoglobulin production from 310 to 560% over that expected on the basis of individual culture data were noted in co-cultures of one of the infants' cells with two different unrelated normal control cells. These findings suggest that that infant may have had a T helper cell defect or that his T cells were unable to produce soluble factors necessary for B cell differentiation. The finding of cells with differentiation markers characteristic of T and B lymphocytes in each of these patients, though in variable quantities, is further evidence for heterogeneity among patients with the clinical syndrome of severe combined immunodeficiency and argues against the concept that their immunodeficiency was due to a stem cell defect.", "contents": "Heterogeneity of lymphocyte subpopulations in severe combined immunodeficiency. Evidence against a stem cell defect. Surface markers typical of T and B lymphocytes were present on varying proportions of peripheral blood lymphocytes from three infants with severe combined immunodeficiency disease. Despite this, functions mediated by T and B cells were either absent or very minimal in all three, including cell-mediated responses in vivo; the in vitro proliferative response to mitogens, allogeneic cells, or antigens; effector cell function in lymphocyte-antibody lymphocytolytic interaction assays; and in vitro synthesis of IgG, IgA, and IgM. In contrast, mononuclear cells from one of the infants were tested and found capable of lysing both human and chicken antibody-coated erythrocyte targets normally. Co-cultivation experiments with unrelated normal control lymphocytes failed to demonstrate suppressor cell activity for immunoglobulin synthesis in these infants. Augmentations of immunoglobulin production from 310 to 560% over that expected on the basis of individual culture data were noted in co-cultures of one of the infants' cells with two different unrelated normal control cells. These findings suggest that that infant may have had a T helper cell defect or that his T cells were unable to produce soluble factors necessary for B cell differentiation. The finding of cells with differentiation markers characteristic of T and B lymphocytes in each of these patients, though in variable quantities, is further evidence for heterogeneity among patients with the clinical syndrome of severe combined immunodeficiency and argues against the concept that their immunodeficiency was due to a stem cell defect."}
{"id": "PMID:1084355", "title": "Radioligand receptor assay for 25-hydroxyvitamin D2/D3 and 1 alpha, 25-dihydroxyvitamin D2/D3.", "content": "A competitive protein binding assay for measurement of the plasma concentration of 1 alpha, 25-dihydroxyvitamin D3 [1alpha, 25-(OH)2D3] has been extended to include the immediate precursor of this hormone, 25-hydroxyvitamin D3 (25-OHD3). In addition, the assay system is capable of measuring the two metabolic products of ergocalciferol, namely. 25-hydroxyvitamin D2 (25-OHD2) and 1alpha, 25-dihydroxyvitamin D2 [1alpha, 25-(OH)2D2]. The target tissue assay system consists of a high affinity cytosol receptor protein that binds the vitamin D metabolites and a limited number of acceptor sites on the nuclear chromatin. By utilizing a series of chromatographic purification steps, a single plasma sample can be assayed for any of the four vitamin D metabolites either individually or combined. Therefore, the assay procedure allows for both the quantitative and qualitative assessment of the total active vitamin D level in a given plasma sample. To show that the binding assay was capable of measuring 1alpha, 25-(OH)2D2 as well as 1alpha, 25 (OH)2D3, two groups of rats were raised. One group, supplemented with vitamin D3, produced assayable material that represented 1alpha, 25-(OH)2D3. The other group, fed only vitamin D2 in the diet, yielded plasma containing only 1alpha, 25-(OH)2D2 as the hormonal form of the vitamin. The circulating concentrations of the two active sterols were nearly identical (15 ng/100 ml) in both groups, indicating that the competitive binding assay can be used to measure both hormonal forms in plasma. In a separate experiment, 1alpha, 25-(OH)2D2 was generated in an in vitro kidney homogenate system using 25-OHD2 as substrate. Comparison of this sterol with 1alpha, 25-(OH)2D3 in the assay system showed very similar binding curves; the D2 form was slightly less efficient (77%). Comparison of the respective 25-hydroxy forms (25-OHD2 vs. 25-OHD3) at concentrations 500-fold that of 1alpha, 25-(OH)2D3, again suggested that the binding of the D2 metabolite was slightly less efficient (71%). Finally, the assay was employed to measure the total active vitamin D metabolite pools in the plasma of normal subjects and patients with varying degrees of hypervitaminosis D. The normal plasma levels of 25-OHD and 1alpha, 25-(OH)2D measured in Tucson adults were 25-40 ng/ml and 2.1-4.5 ng/100 ml, respectively. Both sterols were predominately (greater than 90%) in the form of vitamin D3 metabolites in this environment. Typical cases of hypervitaminosis D exhibited approximately a 15-fold increase in the plasma 25-OHD concentration, and a dramatic changeover to virtually all metabolites existing in the form of D2 vitamins. In contrast, the circulating concentration of 1alpha, 25-(OH)2D was not substantially enhanced in vitamin D-intoxicated patients. We therefore conclude that hypervitaminosis D is not a result of abnormal plasma levels of 1alpha, 25-(OH)2D but may be cuased by an excessive circulating concentration of 25-OHD.", "contents": "Radioligand receptor assay for 25-hydroxyvitamin D2/D3 and 1 alpha, 25-dihydroxyvitamin D2/D3. A competitive protein binding assay for measurement of the plasma concentration of 1 alpha, 25-dihydroxyvitamin D3 [1alpha, 25-(OH)2D3] has been extended to include the immediate precursor of this hormone, 25-hydroxyvitamin D3 (25-OHD3). In addition, the assay system is capable of measuring the two metabolic products of ergocalciferol, namely. 25-hydroxyvitamin D2 (25-OHD2) and 1alpha, 25-dihydroxyvitamin D2 [1alpha, 25-(OH)2D2]. The target tissue assay system consists of a high affinity cytosol receptor protein that binds the vitamin D metabolites and a limited number of acceptor sites on the nuclear chromatin. By utilizing a series of chromatographic purification steps, a single plasma sample can be assayed for any of the four vitamin D metabolites either individually or combined. Therefore, the assay procedure allows for both the quantitative and qualitative assessment of the total active vitamin D level in a given plasma sample. To show that the binding assay was capable of measuring 1alpha, 25-(OH)2D2 as well as 1alpha, 25 (OH)2D3, two groups of rats were raised. One group, supplemented with vitamin D3, produced assayable material that represented 1alpha, 25-(OH)2D3. The other group, fed only vitamin D2 in the diet, yielded plasma containing only 1alpha, 25-(OH)2D2 as the hormonal form of the vitamin. The circulating concentrations of the two active sterols were nearly identical (15 ng/100 ml) in both groups, indicating that the competitive binding assay can be used to measure both hormonal forms in plasma. In a separate experiment, 1alpha, 25-(OH)2D2 was generated in an in vitro kidney homogenate system using 25-OHD2 as substrate. Comparison of this sterol with 1alpha, 25-(OH)2D3 in the assay system showed very similar binding curves; the D2 form was slightly less efficient (77%). Comparison of the respective 25-hydroxy forms (25-OHD2 vs. 25-OHD3) at concentrations 500-fold that of 1alpha, 25-(OH)2D3, again suggested that the binding of the D2 metabolite was slightly less efficient (71%). Finally, the assay was employed to measure the total active vitamin D metabolite pools in the plasma of normal subjects and patients with varying degrees of hypervitaminosis D. The normal plasma levels of 25-OHD and 1alpha, 25-(OH)2D measured in Tucson adults were 25-40 ng/ml and 2.1-4.5 ng/100 ml, respectively. Both sterols were predominately (greater than 90%) in the form of vitamin D3 metabolites in this environment. Typical cases of hypervitaminosis D exhibited approximately a 15-fold increase in the plasma 25-OHD concentration, and a dramatic changeover to virtually all metabolites existing in the form of D2 vitamins. In contrast, the circulating concentration of 1alpha, 25-(OH)2D was not substantially enhanced in vitamin D-intoxicated patients. We therefore conclude that hypervitaminosis D is not a result of abnormal plasma levels of 1alpha, 25-(OH)2D but may be cuased by an excessive circulating concentration of 25-OHD."}
{"id": "PMID:1084356", "title": "Clinical and laboratory studies into the pathogenesis of malacoplakia.", "content": "Three cases of malacoplakia are described. Electron microscopic studies revealed intact bacteria or bacteria in varying states of degradation within phagolysosomes of the malacoplakic macrophages. Michaelis-Gutmann bodies arise within the phagolysosomes. These findings suggest that the bacteria incorporated within the phagolysosomes persist as dense amorphous aggregates which later become encrusted with calcium phosphate crystals to form the laminated Michaelis-Gutmann bodies. Possible explanations for the unusual macrophage response in malacoplakia are: (1) infection with an unusual strain of bacteria, (2) an immunological abnormality affecting intracellular killing of organisms, and (3) an abnormality affecting intracellular digestion of organisms. In considering each of the possibilities, we have been unable to detect any unusual strain of infecting organisms in association with malacoplakia, and in vitro studies have revealed normal T lymphocyte response to mitogen and normal monocyte bactericidal capacity. According to the history, each patient had reason to have a compromised immune status; in only one, however, was this demonstrated.", "contents": "Clinical and laboratory studies into the pathogenesis of malacoplakia. Three cases of malacoplakia are described. Electron microscopic studies revealed intact bacteria or bacteria in varying states of degradation within phagolysosomes of the malacoplakic macrophages. Michaelis-Gutmann bodies arise within the phagolysosomes. These findings suggest that the bacteria incorporated within the phagolysosomes persist as dense amorphous aggregates which later become encrusted with calcium phosphate crystals to form the laminated Michaelis-Gutmann bodies. Possible explanations for the unusual macrophage response in malacoplakia are: (1) infection with an unusual strain of bacteria, (2) an immunological abnormality affecting intracellular killing of organisms, and (3) an abnormality affecting intracellular digestion of organisms. In considering each of the possibilities, we have been unable to detect any unusual strain of infecting organisms in association with malacoplakia, and in vitro studies have revealed normal T lymphocyte response to mitogen and normal monocyte bactericidal capacity. According to the history, each patient had reason to have a compromised immune status; in only one, however, was this demonstrated."}
{"id": "PMID:1084358", "title": "Defective T cell function in atopic dermatitis.", "content": "The cellular immune system of 37 patients with atopic dermatitis (AD) was assessed by measuring peripheral blood T and B cells and the in vitro lymphocyte response to graded doses of phytohemagglutinin (PHA) (background and 6 concentrations of PHA from 100 to 1.6 mug). These were then correlated with clinical severity, ecosinophil counts, and serum IgE levels. The IgE levels (1,482 IU +/- 252 SEM), eosinophil counts (977 +/- 143), and absolute number of B cells (958 +/- 123) were significantly (p less than 0.05) higher than in age-matched controls (70 IU +/- 28, 182 +/- 79, and 480 +/- 60, respectively), and each significantly (p less than 0.05) correlated with the clinical severity. By contrast, percent B lymphocytes (20 +/- 1), percent (51 +/- 2) and total (2,357 +/- 217) T cells did not differ from controls. Eleven patients had low percent T cells (less than 40%); clinical and laboratory evaluation in these patients did not differ from the remaining 26. Lymphocytes from AD patients had higher background deoxyribonucleic acid (DNA) synthesis than controls (suggestive of increased number of B cells) and significantly depressed responses at the low PHA concentrations (6.3, 3.1, and 1.6 mug), which significantly correlated (p less than 0.05) inversely with IgE levels. These studies suggest a subtle defect in T lymphocyte function leading to increased B cells and increased IgE production.", "contents": "Defective T cell function in atopic dermatitis. The cellular immune system of 37 patients with atopic dermatitis (AD) was assessed by measuring peripheral blood T and B cells and the in vitro lymphocyte response to graded doses of phytohemagglutinin (PHA) (background and 6 concentrations of PHA from 100 to 1.6 mug). These were then correlated with clinical severity, ecosinophil counts, and serum IgE levels. The IgE levels (1,482 IU +/- 252 SEM), eosinophil counts (977 +/- 143), and absolute number of B cells (958 +/- 123) were significantly (p less than 0.05) higher than in age-matched controls (70 IU +/- 28, 182 +/- 79, and 480 +/- 60, respectively), and each significantly (p less than 0.05) correlated with the clinical severity. By contrast, percent B lymphocytes (20 +/- 1), percent (51 +/- 2) and total (2,357 +/- 217) T cells did not differ from controls. Eleven patients had low percent T cells (less than 40%); clinical and laboratory evaluation in these patients did not differ from the remaining 26. Lymphocytes from AD patients had higher background deoxyribonucleic acid (DNA) synthesis than controls (suggestive of increased number of B cells) and significantly depressed responses at the low PHA concentrations (6.3, 3.1, and 1.6 mug), which significantly correlated (p less than 0.05) inversely with IgE levels. These studies suggest a subtle defect in T lymphocyte function leading to increased B cells and increased IgE production."}
{"id": "PMID:1084359", "title": "Clinical conference: Severe obstructive lung disease in a 14-year-old girl with alpha-1 antitrypsin deficiency.", "content": "Severe, largely irreversible obstructive lung disease, compatible with emphysema, was found in a 14-yr-old white girl who had been considered to have chronic asthma. She also presented a serum alpha-1 antitrypsin deficiency. Serum values for alpha-1 antitrypsin on two occassions were 95 and 105 mg/100 ml; Pi type was SZ. A family survey disclosed a 13-yr-old brother with the same pattern of alpha-1 antitrypsin  deficiency. His serum value was 122.5 mg/100 ml; Pi type was SZ. He was asymptomatic and showed minimal pulmonary function abnormalities.", "contents": "Clinical conference: Severe obstructive lung disease in a 14-year-old girl with alpha-1 antitrypsin deficiency. Severe, largely irreversible obstructive lung disease, compatible with emphysema, was found in a 14-yr-old white girl who had been considered to have chronic asthma. She also presented a serum alpha-1 antitrypsin deficiency. Serum values for alpha-1 antitrypsin on two occassions were 95 and 105 mg/100 ml; Pi type was SZ. A family survey disclosed a 13-yr-old brother with the same pattern of alpha-1 antitrypsin  deficiency. His serum value was 122.5 mg/100 ml; Pi type was SZ. He was asymptomatic and showed minimal pulmonary function abnormalities."}
{"id": "PMID:1084360", "title": "Interaction between lymphocytes and inflammatory exudate cells. II. A proteolytic enzyme released by PMN as a possible mediator for enhancement of thymocyte response.", "content": "Mouse polymorphonuclear leukocytes (PMN) were harvested from the peritoneal cavity stimulated with sodium caseinate. The cells were cultivated in vitro and the supernatant of these cultures (SUP) was tested for enhancing potency on DNA synthesis by syngeneic thymocytes stimulated with phytohemagglutinin (PHA). The enhancing potency of the SUP was markedly influenced by duration of the donor cultures of PMN, population density of the cultures, and protein concentrations in the medium, respectively. The enhancing factor in the SUP was found to be non-dialysable, heat-labile, and stable in the pH ranging between 3 and 9 but labile in the pH below 2 or above 10; its m.w. was approximately 19.000 when measured by gel-filtration on Sephadex G-75. The factor had a proteolytic activity on 3H-acetyl hemoglobin (3HHb) at neutral pH (7.2). Both the proteolytic activity and thymocyte-helping potency of the SUP were similarly abolished by adding protease inhibitor (Trasylol) in soluble form, or by passing through a column of the inhibitor insolubilized. It was thus assumed that the enhancing effect of PMN on thymocyte response was associated with a neutral protease released from the cells: it may be termed a lymphocyte-helping protease (LHP).", "contents": "Interaction between lymphocytes and inflammatory exudate cells. II. A proteolytic enzyme released by PMN as a possible mediator for enhancement of thymocyte response. Mouse polymorphonuclear leukocytes (PMN) were harvested from the peritoneal cavity stimulated with sodium caseinate. The cells were cultivated in vitro and the supernatant of these cultures (SUP) was tested for enhancing potency on DNA synthesis by syngeneic thymocytes stimulated with phytohemagglutinin (PHA). The enhancing potency of the SUP was markedly influenced by duration of the donor cultures of PMN, population density of the cultures, and protein concentrations in the medium, respectively. The enhancing factor in the SUP was found to be non-dialysable, heat-labile, and stable in the pH ranging between 3 and 9 but labile in the pH below 2 or above 10; its m.w. was approximately 19.000 when measured by gel-filtration on Sephadex G-75. The factor had a proteolytic activity on 3H-acetyl hemoglobin (3HHb) at neutral pH (7.2). Both the proteolytic activity and thymocyte-helping potency of the SUP were similarly abolished by adding protease inhibitor (Trasylol) in soluble form, or by passing through a column of the inhibitor insolubilized. It was thus assumed that the enhancing effect of PMN on thymocyte response was associated with a neutral protease released from the cells: it may be termed a lymphocyte-helping protease (LHP)."}
{"id": "PMID:1084361", "title": "Identification of T and B cell subpopulations in human peripheral blood: electrophoretic mobility distributions associated with surface marker characteristics.", "content": "The electrophoretic mobility distributions of human peripheral blood lymphocytes isolated on Ficoll-Hypaque gradients were characterized by laser Doppler spectroscopy. Three major subpopulations were spectrally resolved, due to differences in their mobility, when an electric field was applied to the scattering cuvette. The fastest component (centered at 2.35 mum/sec/V/cm for 25 degrees C, 0.28 M sucrose medium of 0.005 ionic strength) passed through nylon fiber columns and was identified as a T cell subpopulation. The slowest component (1.85 mum/sec/V/cm) which was further enriched by a one-step rosette procedure with sheep erythrocytes, reacted with activated-complement (C3) and antiserum to human immunoglobulins and was therefore identified as a B cell subpopulation. The intermediate component (centered at 2.15 mum/sec/V/cm) appears to be another T cellsubpopulation. Although cells of this component did not pass through nylon fiber columns, they did rosette with sheep erythrocytes. Furthermore, these cells did not appear to have surface immunoglobulins or complement receptors.", "contents": "Identification of T and B cell subpopulations in human peripheral blood: electrophoretic mobility distributions associated with surface marker characteristics. The electrophoretic mobility distributions of human peripheral blood lymphocytes isolated on Ficoll-Hypaque gradients were characterized by laser Doppler spectroscopy. Three major subpopulations were spectrally resolved, due to differences in their mobility, when an electric field was applied to the scattering cuvette. The fastest component (centered at 2.35 mum/sec/V/cm for 25 degrees C, 0.28 M sucrose medium of 0.005 ionic strength) passed through nylon fiber columns and was identified as a T cell subpopulation. The slowest component (1.85 mum/sec/V/cm) which was further enriched by a one-step rosette procedure with sheep erythrocytes, reacted with activated-complement (C3) and antiserum to human immunoglobulins and was therefore identified as a B cell subpopulation. The intermediate component (centered at 2.15 mum/sec/V/cm) appears to be another T cellsubpopulation. Although cells of this component did not pass through nylon fiber columns, they did rosette with sheep erythrocytes. Furthermore, these cells did not appear to have surface immunoglobulins or complement receptors."}
{"id": "PMID:1084362", "title": "Mouse lymphotoxin.", "content": "The addition of PHA to C3H mouse spleen cells in tissue culture led to the production of lymphotoxin (LT). Cytotoxicity was assayed by addition of the culture fluids to syngeneic target cells labeled with tritiated thymidine; after an incubation period of 72 hr the amount of radioactivity released into the supernatant was measured. The LT activity in unfractionated culture fluids survived lyophilization, remained unchanged for many weeks at 4 degrees C, and progressively decreased on heating at 56 degrees C for periods from 1 to 9 hr. Based on the G-200 Sephadex distribution coefficients for several preparations, the m.w. of mouse lymphotoxin was about 41,000 daltons. Lymphotoxin from three different spleen cell production runs was recovered from isoelectric focusing columns in sharply focused peaks, the pH of which ranged from 4.4 to 4.8.", "contents": "Mouse lymphotoxin. The addition of PHA to C3H mouse spleen cells in tissue culture led to the production of lymphotoxin (LT). Cytotoxicity was assayed by addition of the culture fluids to syngeneic target cells labeled with tritiated thymidine; after an incubation period of 72 hr the amount of radioactivity released into the supernatant was measured. The LT activity in unfractionated culture fluids survived lyophilization, remained unchanged for many weeks at 4 degrees C, and progressively decreased on heating at 56 degrees C for periods from 1 to 9 hr. Based on the G-200 Sephadex distribution coefficients for several preparations, the m.w. of mouse lymphotoxin was about 41,000 daltons. Lymphotoxin from three different spleen cell production runs was recovered from isoelectric focusing columns in sharply focused peaks, the pH of which ranged from 4.4 to 4.8."}
{"id": "PMID:1084363", "title": "Studies on the maturation of immune responsiveness in the mouse. II. Role of the spleen.", "content": "Experiments were designed to investigate the role of the spleen in the development of the murine immune system. By using mice splenectomized within 24 hr of birth, as well as mice with a hereditary, congenital absence of the spleen, the primary immune response to sheep erythrocytes was examined. The immunocompetence of lymph node cells from spleenless or control mice was assessed in vitro, in organ and in cell suspension cultures, and in vivo, by transfer into lethally irradiated syngeneic recipients followed by antigenic stimulation. The immunologic capacities of thymus and bone marrow cells were similarly tested by injection separately or in combination into irradiated syngeneic mice. Lymph node cells from spleenless animals appeared fully competent both in vitro and in transfer experiments. Neither neonatal splenectomy nor congenital absence of the spleen significantly reduced the capacity of bone marrow or thymus cells to participate in the immune response to sheep erythrocytes.", "contents": "Studies on the maturation of immune responsiveness in the mouse. II. Role of the spleen. Experiments were designed to investigate the role of the spleen in the development of the murine immune system. By using mice splenectomized within 24 hr of birth, as well as mice with a hereditary, congenital absence of the spleen, the primary immune response to sheep erythrocytes was examined. The immunocompetence of lymph node cells from spleenless or control mice was assessed in vitro, in organ and in cell suspension cultures, and in vivo, by transfer into lethally irradiated syngeneic recipients followed by antigenic stimulation. The immunologic capacities of thymus and bone marrow cells were similarly tested by injection separately or in combination into irradiated syngeneic mice. Lymph node cells from spleenless animals appeared fully competent both in vitro and in transfer experiments. Neither neonatal splenectomy nor congenital absence of the spleen significantly reduced the capacity of bone marrow or thymus cells to participate in the immune response to sheep erythrocytes."}
{"id": "PMID:1084364", "title": "Relationship of CIF, LT, and PIF released in vitro by activated human lymphocytes. II. A further functional comparison of LT and PIF activities on HeLa and L-929 target cells.", "content": "Lymphotoxin (LT), and proliferation inhibition factor (PIF) activities found in 5-day supernatants of mitogen-activated human lymphocytes (SAL) were further compared. In agreement with previous results, the activities could not be distinguished functionally. Quantitative differences in the amount of activity detected in the SAL could be accounted for on the basis of target cell differences, concentration of the lymphocyte effector molecules in the supernatant, and the parameter employed to assess cell function. Growth inhibitory activity detected at high supernatant dilutions was completely reversible, whereas the cytotoxic activity detected at low supernatant dilutions was irreversible. When the active medium was fractionated on DEAE, two peaks of inhibitory activity were detected. Depending upon the amount of activity and target cell, both peaks of activity were growth inhibitory or cytotoxic. Since both peaks of material affected HeLa and L-929 cells, the materials were not species specific. Thus, it appears that cloning inhibitor factor, LT, and PIF activities may actually be measures of the same stable materials found in 5-day activated lymphocyte supernatants.", "contents": "Relationship of CIF, LT, and PIF released in vitro by activated human lymphocytes. II. A further functional comparison of LT and PIF activities on HeLa and L-929 target cells. Lymphotoxin (LT), and proliferation inhibition factor (PIF) activities found in 5-day supernatants of mitogen-activated human lymphocytes (SAL) were further compared. In agreement with previous results, the activities could not be distinguished functionally. Quantitative differences in the amount of activity detected in the SAL could be accounted for on the basis of target cell differences, concentration of the lymphocyte effector molecules in the supernatant, and the parameter employed to assess cell function. Growth inhibitory activity detected at high supernatant dilutions was completely reversible, whereas the cytotoxic activity detected at low supernatant dilutions was irreversible. When the active medium was fractionated on DEAE, two peaks of inhibitory activity were detected. Depending upon the amount of activity and target cell, both peaks of activity were growth inhibitory or cytotoxic. Since both peaks of material affected HeLa and L-929 cells, the materials were not species specific. Thus, it appears that cloning inhibitor factor, LT, and PIF activities may actually be measures of the same stable materials found in 5-day activated lymphocyte supernatants."}
{"id": "PMID:1084365", "title": "The requirement for esterase activation in the anti-immunoglobulin-triggered movement of B lymphocytes.", "content": "In this paper we bring evidence suggesting that there is activation of an esterase upon reaction of anti-immunoglobulin antibodies (anti-Ig) with murine B lymphocytes. B lymphocytes upon exposure to anti-Ig cap the ligand-receptor complexes and immediately afterward become briefly motile. It is this latter step which is inhibitable by exposure to di-isopropyl phosphofluoridate (DFP). Various experimental manipulations indicated that treatment with anti-Ig activates the cell for motility which, however, is not manifested until the temperature is raised to 37 degrees C. The cell incubated with anti-Ig at cold temperatures becomes susceptible to the effect of DFP, suggesting that the antibody-treated cells are activated up to but not beyond the DFP inhibitable step. Exposure of cells to DFP and removal of it before their treatment with anti-Ig does not affect the anti-Ig-induced response. Four lines of evidence indicate that the reduction of lymphocyte movement of DFP is due to the inhibition of an esterase activated by the combination of antibody and cell: 1) The inhibition by DFP is irreversible; once DFP has reacted it can be washed away and the antibody-treated cell is still inhibited. 2) The inhibition increases with time of contact of lymphocytes and DFP and with the concentration of DFP. 3) A very poorly phosphorylating phosphonate, phenyl ethyl pentylphosphonate is completely inactive under conditions where an excellent phosphorylating phosphonate, p nitrophenyl ethyl pentylphosphonate maximally inactivated the cells' movement. 4) The amino acid esters, tosyl L arginine methyl ester and benzoyl arginine methyl ester specifically prevent the inactivation by DFP. The last finding suggests that tosyl L arginine methyl ester and benzoyl L arginine methyl ester might be substrates for the putative antibody-induced lymphocyte esterase. Lymphocytes incubated with antibody in the cold for more than 30 min lose their ability to move when the temperature is raised, suggesting that there is a time-dependent deactivation of the cell.", "contents": "The requirement for esterase activation in the anti-immunoglobulin-triggered movement of B lymphocytes. In this paper we bring evidence suggesting that there is activation of an esterase upon reaction of anti-immunoglobulin antibodies (anti-Ig) with murine B lymphocytes. B lymphocytes upon exposure to anti-Ig cap the ligand-receptor complexes and immediately afterward become briefly motile. It is this latter step which is inhibitable by exposure to di-isopropyl phosphofluoridate (DFP). Various experimental manipulations indicated that treatment with anti-Ig activates the cell for motility which, however, is not manifested until the temperature is raised to 37 degrees C. The cell incubated with anti-Ig at cold temperatures becomes susceptible to the effect of DFP, suggesting that the antibody-treated cells are activated up to but not beyond the DFP inhibitable step. Exposure of cells to DFP and removal of it before their treatment with anti-Ig does not affect the anti-Ig-induced response. Four lines of evidence indicate that the reduction of lymphocyte movement of DFP is due to the inhibition of an esterase activated by the combination of antibody and cell: 1) The inhibition by DFP is irreversible; once DFP has reacted it can be washed away and the antibody-treated cell is still inhibited. 2) The inhibition increases with time of contact of lymphocytes and DFP and with the concentration of DFP. 3) A very poorly phosphorylating phosphonate, phenyl ethyl pentylphosphonate is completely inactive under conditions where an excellent phosphorylating phosphonate, p nitrophenyl ethyl pentylphosphonate maximally inactivated the cells' movement. 4) The amino acid esters, tosyl L arginine methyl ester and benzoyl arginine methyl ester specifically prevent the inactivation by DFP. The last finding suggests that tosyl L arginine methyl ester and benzoyl L arginine methyl ester might be substrates for the putative antibody-induced lymphocyte esterase. Lymphocytes incubated with antibody in the cold for more than 30 min lose their ability to move when the temperature is raised, suggesting that there is a time-dependent deactivation of the cell."}
{"id": "PMID:1084366", "title": "Regulatory factors produced by lymphocytes. I. The occurrence of multiple alpha-lymphotoxins associated with ribonuclease activity.", "content": "Supernatant fluids of mitogen-activated human tonsil lymphocytes contain large amounts of a factor toxic to mouse L cells. This substance, with a m.w. of 80,000 +/- 5,000 daltons, is called alpha-lymphotoxin (alpha-LT), to differentiate it from another toxin elaborated by mitogen activated human blood lymphocytes, called beta-lymphotoxin (beta-LT), which differs from alpha-LT in size (45,000 +/- 5,000 daltons), antigenicity, and stability. Further purification of alpha-LT by sequential phosphocellulose and DEAE-cellulose chromatography and polyacrylamide gel electrophoresis (PAGE) identifies a series of cytotoxins differing in ion exchange characteristics and electrophoretic mobilities. The three PAGE fractions (PAGE Ia, Ib and II), recovered in 2, 4.6, and 21% yield from the starting serum-free culture supernatant, represent purifications of 24-, 24- and 1851-fold, respectively. Each cytotoxic fraction has a ribonuclease activity. Comparison of RNase and mouse L cell cytotoxic activities of the three alpha-LT fractions shows that both activities for all three fractions have a similar temperature stability pattern and that both are similarly inhibited by DNA, single strand forms better than double strands, by glycerol in 5 to 20% concentration, and by protein denaturing reagents. These observations suggest, but do not prove, that mouse L cell toxicity and RNase activity are mediated by the same substance, which appears to occur in multiple or isozymic forms.", "contents": "Regulatory factors produced by lymphocytes. I. The occurrence of multiple alpha-lymphotoxins associated with ribonuclease activity. Supernatant fluids of mitogen-activated human tonsil lymphocytes contain large amounts of a factor toxic to mouse L cells. This substance, with a m.w. of 80,000 +/- 5,000 daltons, is called alpha-lymphotoxin (alpha-LT), to differentiate it from another toxin elaborated by mitogen activated human blood lymphocytes, called beta-lymphotoxin (beta-LT), which differs from alpha-LT in size (45,000 +/- 5,000 daltons), antigenicity, and stability. Further purification of alpha-LT by sequential phosphocellulose and DEAE-cellulose chromatography and polyacrylamide gel electrophoresis (PAGE) identifies a series of cytotoxins differing in ion exchange characteristics and electrophoretic mobilities. The three PAGE fractions (PAGE Ia, Ib and II), recovered in 2, 4.6, and 21% yield from the starting serum-free culture supernatant, represent purifications of 24-, 24- and 1851-fold, respectively. Each cytotoxic fraction has a ribonuclease activity. Comparison of RNase and mouse L cell cytotoxic activities of the three alpha-LT fractions shows that both activities for all three fractions have a similar temperature stability pattern and that both are similarly inhibited by DNA, single strand forms better than double strands, by glycerol in 5 to 20% concentration, and by protein denaturing reagents. These observations suggest, but do not prove, that mouse L cell toxicity and RNase activity are mediated by the same substance, which appears to occur in multiple or isozymic forms."}
{"id": "PMID:1084367", "title": "C1q (c1) receptor on human platelets: inhibition of collagen-induced platelet aggregation by C1q (C1) molecules.", "content": "Hemolytically active human C1q incubated with EA before the addition of complement inhibited the immune hemolysis. On the contrary, heat-inactivated preparation (30 min 56 degrees C) was ineffective. Preincubation of EA with bovine collagen also resulted in a decreased hemolysis. When aggregation was measured by a turbidimetric method in citrated human platelet-rich plasma, it was found that hemolytically active human C1q (C1) alone does not induce platelet aggregation. However, in its presence the platelets failed to aggregate or exhibited a significantly reduced aggregation response to bovine collagen. The inhibition by C1q depended on the preincubation time with platelets. Heat treatment (30 min 56 degrees C) destroyed the inhibitory action of C1q (C1). The effect of C1q proved to be highly specific because different C1q preparations at their inhibitory doses in collagen-induced platelet aggregation did not influence the response to other aggregating agents (bovine thrombin, ADP, horse anti-human thymocyte globulin, goat anti-baboon platelet antiserum). The results prove that collagen and C1q are capable of binding to the same site(s); namely, to those of EA and human platelets; furthermore, they suggest the presence of a receptor for C1q (C1) on human platelets.", "contents": "C1q (c1) receptor on human platelets: inhibition of collagen-induced platelet aggregation by C1q (C1) molecules. Hemolytically active human C1q incubated with EA before the addition of complement inhibited the immune hemolysis. On the contrary, heat-inactivated preparation (30 min 56 degrees C) was ineffective. Preincubation of EA with bovine collagen also resulted in a decreased hemolysis. When aggregation was measured by a turbidimetric method in citrated human platelet-rich plasma, it was found that hemolytically active human C1q (C1) alone does not induce platelet aggregation. However, in its presence the platelets failed to aggregate or exhibited a significantly reduced aggregation response to bovine collagen. The inhibition by C1q depended on the preincubation time with platelets. Heat treatment (30 min 56 degrees C) destroyed the inhibitory action of C1q (C1). The effect of C1q proved to be highly specific because different C1q preparations at their inhibitory doses in collagen-induced platelet aggregation did not influence the response to other aggregating agents (bovine thrombin, ADP, horse anti-human thymocyte globulin, goat anti-baboon platelet antiserum). The results prove that collagen and C1q are capable of binding to the same site(s); namely, to those of EA and human platelets; furthermore, they suggest the presence of a receptor for C1q (C1) on human platelets."}
{"id": "PMID:1084368", "title": "Suppressor cells in spleens from \"nude\" mice: their effect on the mitogenic response of B lymphocytes.", "content": "A cell population recovered after velocity sedimentation fractionation of \"nude\" but not haired mouse spleen cells suppressed the response of spleen cells from both \"nude\" and haired mice to the B cell mitogen, LPS. The suppressor activity of these cells was abrogated by treatment with anti-theta antiserum and complement but not by treatment with the same antiserum preabsorbed with mouse brain. It is possible that these suppressor cells come from the pool of T cell precursors known to be present in \"nude\" bone marrow and spleen, and that they do not require thymic influence in order to perform the suppressor function.", "contents": "Suppressor cells in spleens from \"nude\" mice: their effect on the mitogenic response of B lymphocytes. A cell population recovered after velocity sedimentation fractionation of \"nude\" but not haired mouse spleen cells suppressed the response of spleen cells from both \"nude\" and haired mice to the B cell mitogen, LPS. The suppressor activity of these cells was abrogated by treatment with anti-theta antiserum and complement but not by treatment with the same antiserum preabsorbed with mouse brain. It is possible that these suppressor cells come from the pool of T cell precursors known to be present in \"nude\" bone marrow and spleen, and that they do not require thymic influence in order to perform the suppressor function."}
{"id": "PMID:1084369", "title": "Isolation and partial characterization of AgF-1:a rat lymphocyte membrane antigen.", "content": "A genetically defined, serologically identified antigen of the rat lymphocyte membrane (AgF-1) has been isolated. Viable spleen and lymph node cells, prepared by Ficoll-Hypaque density centrifugation from Fischer rats, were radioiodinated with soluble lactoperoxidase. Extracts obtained with Nonidet P-40 were shown to contain numerous radiolabeled proteins including cell-surface globulin. AgF-1 was isolated from these extracts by precipitation with a highly specific alloantibody in conjunction with xenospecific anti-globulin antibody and polyethylene glycol (PEG). The use of PEG greatly increased the efficiency of the double antibody technique. The putative antigenic peak was eluted from sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and specific antigenic activity was recovered. Removal of the SDS from these eluates was achieved by equilibration with urea and passage over an anion exchange resin. Renaturation, as evidenced by specific inhibition of complement-mediated cytotoxicity, occurred upon the removal of urea by dialysis. The m.w. of the purified antigen was estimated to be 35 to 40,000 daltons by SDS-PAGE and was unaffected by reduction with 2-mercaptoethanol. Amino acid composition was roughly similar to those reported for the major histocompatibility antigens of the rat and other species.", "contents": "Isolation and partial characterization of AgF-1:a rat lymphocyte membrane antigen. A genetically defined, serologically identified antigen of the rat lymphocyte membrane (AgF-1) has been isolated. Viable spleen and lymph node cells, prepared by Ficoll-Hypaque density centrifugation from Fischer rats, were radioiodinated with soluble lactoperoxidase. Extracts obtained with Nonidet P-40 were shown to contain numerous radiolabeled proteins including cell-surface globulin. AgF-1 was isolated from these extracts by precipitation with a highly specific alloantibody in conjunction with xenospecific anti-globulin antibody and polyethylene glycol (PEG). The use of PEG greatly increased the efficiency of the double antibody technique. The putative antigenic peak was eluted from sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and specific antigenic activity was recovered. Removal of the SDS from these eluates was achieved by equilibration with urea and passage over an anion exchange resin. Renaturation, as evidenced by specific inhibition of complement-mediated cytotoxicity, occurred upon the removal of urea by dialysis. The m.w. of the purified antigen was estimated to be 35 to 40,000 daltons by SDS-PAGE and was unaffected by reduction with 2-mercaptoethanol. Amino acid composition was roughly similar to those reported for the major histocompatibility antigens of the rat and other species."}
{"id": "PMID:1084370", "title": "Generation of specific helper cells and suppressor cells in vitro for the IgE and IgG antibody responses.", "content": "Normal splenic lymphocytes from BDF1 mice were cultured on ovalbumin (OA)-bearing syngeneic peritoneal adherent cells for 5 days and their subsequent helper function was tested by an adoptive transfer technique. Lymphocytes harvested from the culture were mixed with DNP-KLH-primed spleen cells and transferred into irradiated syngeneic mice followed by challenge with DNP-OA. The results showed that the cultured lymphocytes has helper function for both IgE and IgG anti-DNP antibody responses. Depletion of mast cells and T cells in the peritoneal adherent cell preparations did not affect the generation of helper cells in the culture. The helper function of the cultured lymphocytes was abolished by the treatment with anti-theta antiserum and complement and was specific for ovalbumin. The OA-specific helper T cells were generated in vitro by the culture of a T cell-rich fraction of normal spleen on T cell-depleted OA-bearing peritoneal cells. If the normal splenic lymphocytes or T cell-rich fraction were cultured with 10 mug/ml of OA in the absence of macrophages, cultured lymphocytes lacked helper function. The transfer of splenic lymphocytes or splenic T cells cultured with soluble OA to normal non-irradiated mice, however, suppressed both IgG and IgE antibody responses of the recipients to subsequent immunization with DNP-OA. The suppressor cells were sensitive to anti-theta antiserum and complement and their activity was specific for OA. The cultured cells transferred into normal mice did not suppress anti-hapten antibody response to DNP-KLH. Normal lymphocytes cultured on OA-bearing macrophages and had helper function in adoptive transfer experiments failed to suppress antibody response of non-irradiated recipients to DNP-OA. The results indicate that OA-bearing macrophages primed T cells and generated helper T cells, whereas the culture of normal lymphocytes with soluble OA in the absence of macrophages generated suppressor T cells.", "contents": "Generation of specific helper cells and suppressor cells in vitro for the IgE and IgG antibody responses. Normal splenic lymphocytes from BDF1 mice were cultured on ovalbumin (OA)-bearing syngeneic peritoneal adherent cells for 5 days and their subsequent helper function was tested by an adoptive transfer technique. Lymphocytes harvested from the culture were mixed with DNP-KLH-primed spleen cells and transferred into irradiated syngeneic mice followed by challenge with DNP-OA. The results showed that the cultured lymphocytes has helper function for both IgE and IgG anti-DNP antibody responses. Depletion of mast cells and T cells in the peritoneal adherent cell preparations did not affect the generation of helper cells in the culture. The helper function of the cultured lymphocytes was abolished by the treatment with anti-theta antiserum and complement and was specific for ovalbumin. The OA-specific helper T cells were generated in vitro by the culture of a T cell-rich fraction of normal spleen on T cell-depleted OA-bearing peritoneal cells. If the normal splenic lymphocytes or T cell-rich fraction were cultured with 10 mug/ml of OA in the absence of macrophages, cultured lymphocytes lacked helper function. The transfer of splenic lymphocytes or splenic T cells cultured with soluble OA to normal non-irradiated mice, however, suppressed both IgG and IgE antibody responses of the recipients to subsequent immunization with DNP-OA. The suppressor cells were sensitive to anti-theta antiserum and complement and their activity was specific for OA. The cultured cells transferred into normal mice did not suppress anti-hapten antibody response to DNP-KLH. Normal lymphocytes cultured on OA-bearing macrophages and had helper function in adoptive transfer experiments failed to suppress antibody response of non-irradiated recipients to DNP-OA. The results indicate that OA-bearing macrophages primed T cells and generated helper T cells, whereas the culture of normal lymphocytes with soluble OA in the absence of macrophages generated suppressor T cells."}
{"id": "PMID:1084371", "title": "Antibodies to guinea pig lymphokines. II. Suppression of delayed hypersensitivity reactions by a \"second generation\" goat antibody against guinea pig lymphokines.", "content": "A \"second generation\" antibody to a highly purified lymphocyte product was raised in a goat against material eluted from a rabbit anti-guinea pig lymphokine immunoadsorbent column. This anti-lymphokine serum, in constrast to anti-lymphocyte serum (ALS) did not appear to contain cytotoxic antibodies directed against membrane antigens on guinea pig lymph node lymphocytes. Furthermore, the anti-lymphokine serum did not inhibit the formation of spontaneous T rosettes nor significantly depress lymphocyte response to mitogens. The anti-lymphokine serum totally suppressed the delayed skin reactivity to PPD and contact sensitivity to DNCB when injected intradermally around the site of antigen challenge. By contrast, intradermally injected ALS did not appear to suppress the PPD response in sensitized guinea pigs. Intravenously and i.p. administered anti-lymphokine serum was somewhat less effective in suppressing the delayed skin response to PPD. The intradermal injection of the antiserum had no effect on nonspecific inflammation evoked by turpentine-olive oil or on the extravasation of circulating Evans blue evoked by intradermally injected histamine. Histologic examination of 24-hr DNCB-induced skin lesions from sensitized guinea pigs treated with intradermally injected anti-lymphokine serum showed marked reduction of mononuclear infiltration of the dermis and of epidermal lesions, as compared with skin sites taken from sensitized animals pretreated with normal goat serum. The anti-lymphokine serum injected i.v. also markedly reduced the perivascular infiltration of the dermis and subcutis in skin reaction sites from sensitized animals challenged with PPD. Intravenous treatment with ALS for 3 consecutive days caused extensive depletion of the paracortical areas of peripheral lymph nodes whereas treatment with normal serum and anti-lymphokine serum caused no such depletion. It is proposed that the anti-lymphokine serum is directed against activated lymphocyte products, one of them being MIF. These products are involved in the mediation of delayed hypersensitivity reactions. This is in marked contrast to ALS, the suppressive action of which appears to be central rather than peripheral.", "contents": "Antibodies to guinea pig lymphokines. II. Suppression of delayed hypersensitivity reactions by a \"second generation\" goat antibody against guinea pig lymphokines. A \"second generation\" antibody to a highly purified lymphocyte product was raised in a goat against material eluted from a rabbit anti-guinea pig lymphokine immunoadsorbent column. This anti-lymphokine serum, in constrast to anti-lymphocyte serum (ALS) did not appear to contain cytotoxic antibodies directed against membrane antigens on guinea pig lymph node lymphocytes. Furthermore, the anti-lymphokine serum did not inhibit the formation of spontaneous T rosettes nor significantly depress lymphocyte response to mitogens. The anti-lymphokine serum totally suppressed the delayed skin reactivity to PPD and contact sensitivity to DNCB when injected intradermally around the site of antigen challenge. By contrast, intradermally injected ALS did not appear to suppress the PPD response in sensitized guinea pigs. Intravenously and i.p. administered anti-lymphokine serum was somewhat less effective in suppressing the delayed skin response to PPD. The intradermal injection of the antiserum had no effect on nonspecific inflammation evoked by turpentine-olive oil or on the extravasation of circulating Evans blue evoked by intradermally injected histamine. Histologic examination of 24-hr DNCB-induced skin lesions from sensitized guinea pigs treated with intradermally injected anti-lymphokine serum showed marked reduction of mononuclear infiltration of the dermis and of epidermal lesions, as compared with skin sites taken from sensitized animals pretreated with normal goat serum. The anti-lymphokine serum injected i.v. also markedly reduced the perivascular infiltration of the dermis and subcutis in skin reaction sites from sensitized animals challenged with PPD. Intravenous treatment with ALS for 3 consecutive days caused extensive depletion of the paracortical areas of peripheral lymph nodes whereas treatment with normal serum and anti-lymphokine serum caused no such depletion. It is proposed that the anti-lymphokine serum is directed against activated lymphocyte products, one of them being MIF. These products are involved in the mediation of delayed hypersensitivity reactions. This is in marked contrast to ALS, the suppressive action of which appears to be central rather than peripheral."}
{"id": "PMID:1084372", "title": "Induction of T cell response to haptens coupled to mycobacteria.", "content": "A new method for the induction of cellular immune response to commonly used haptens in the absence of detectable antibody response is described. Different haptens were convalently coupled to Mycobacteria and they were injected into guinea pigs in incomplete Freund's adjuvant. Humoral and cellular immune response to haptens were examined at weekly intervals for 5 weeks. Our results show that a significant anti-hapten cellular response was induced and subsequently elicited by both in vivo (skin test) and in vitro (Lymphocyte transformation and macrophage migration inhibition) assays.", "contents": "Induction of T cell response to haptens coupled to mycobacteria. A new method for the induction of cellular immune response to commonly used haptens in the absence of detectable antibody response is described. Different haptens were convalently coupled to Mycobacteria and they were injected into guinea pigs in incomplete Freund's adjuvant. Humoral and cellular immune response to haptens were examined at weekly intervals for 5 weeks. Our results show that a significant anti-hapten cellular response was induced and subsequently elicited by both in vivo (skin test) and in vitro (Lymphocyte transformation and macrophage migration inhibition) assays."}
{"id": "PMID:1084373", "title": "Automated counting of T cell rosettes.", "content": "Human T cell rosettes were enumerated using an automated particle counter, the Bio/Physics Cytograf 6300A. An electronic oscilloscope representation of particle absorbance and scatter of a focused laser beam allows the separation and enumeration of both rosetted and non-rosetted lymphocytes. Repeated Cytograf sampling of a single rosette preparation gave highly reproducible results, and sampling from replicate tubes produced the same degree of variation as microscopic analysis. T cell rosettes prepared from 27 volunteers and compared by both methods of quantitation showed a high degree of correlation. This method can objectively measure at least 100 times as many cells for their rosette-forming capability as the tedious microscopic technique.", "contents": "Automated counting of T cell rosettes. Human T cell rosettes were enumerated using an automated particle counter, the Bio/Physics Cytograf 6300A. An electronic oscilloscope representation of particle absorbance and scatter of a focused laser beam allows the separation and enumeration of both rosetted and non-rosetted lymphocytes. Repeated Cytograf sampling of a single rosette preparation gave highly reproducible results, and sampling from replicate tubes produced the same degree of variation as microscopic analysis. T cell rosettes prepared from 27 volunteers and compared by both methods of quantitation showed a high degree of correlation. This method can objectively measure at least 100 times as many cells for their rosette-forming capability as the tedious microscopic technique."}
{"id": "PMID:1084374", "title": "Some methodological studies on effector cells in the lymphocyte-dependent cytotoxic antibody assay.", "content": "A method of direct freezing was found to be reliable for storage of the effectors for the LDA assay, though there is a considerable loss of cells. In order to get immunologically active K cells after thawing, an important step is to leave the cells to recuperate overnight in an appropriate medium before use. A short-term storage of fresh effectors is also possible. The use of a pool of effector cells from a minimum of 3 random blood donors obviates the selection of HL-A types furthermore permits a saving of the amount of serum used. Enrichment of a B cell population by removing the cells capable of forming spontaneous rosettes with SRBC was not found to enhance cytotoxicity. Pretreatment of cells with pronase, trypsin and neuraminidase did not significantly enhance their effector activity, while papain treated cells showed a systematically increased specific lysis.", "contents": "Some methodological studies on effector cells in the lymphocyte-dependent cytotoxic antibody assay. A method of direct freezing was found to be reliable for storage of the effectors for the LDA assay, though there is a considerable loss of cells. In order to get immunologically active K cells after thawing, an important step is to leave the cells to recuperate overnight in an appropriate medium before use. A short-term storage of fresh effectors is also possible. The use of a pool of effector cells from a minimum of 3 random blood donors obviates the selection of HL-A types furthermore permits a saving of the amount of serum used. Enrichment of a B cell population by removing the cells capable of forming spontaneous rosettes with SRBC was not found to enhance cytotoxicity. Pretreatment of cells with pronase, trypsin and neuraminidase did not significantly enhance their effector activity, while papain treated cells showed a systematically increased specific lysis."}
{"id": "PMID:1084375", "title": "Determination of DNA antibodies in normal and pathological sera by a new counterimmunoelectrophoresis method.", "content": "A quantitative counter-immunoelectrophoresis technique has been applied to the evaluation of antibodies against native and single-stranded DNA. Anti-DNA antibodies have been found at high dilutions in patients with systematic lupus erythematosus, without correlation with the existence of renal lesions or with the degree of DNA binding assessed by Farr assay. Significant precipitates were also observed at significantly lower dilutions in other pathological situations and in normal subjects, posing the problem of the nature of the precipitates in these cases.", "contents": "Determination of DNA antibodies in normal and pathological sera by a new counterimmunoelectrophoresis method. A quantitative counter-immunoelectrophoresis technique has been applied to the evaluation of antibodies against native and single-stranded DNA. Anti-DNA antibodies have been found at high dilutions in patients with systematic lupus erythematosus, without correlation with the existence of renal lesions or with the degree of DNA binding assessed by Farr assay. Significant precipitates were also observed at significantly lower dilutions in other pathological situations and in normal subjects, posing the problem of the nature of the precipitates in these cases."}
{"id": "PMID:1084376", "title": "Lymphocyte effector molecules: an vitro production method for obtaining liter volumes of supernatants from mitogen-stimulated human lymphocytes.", "content": "An in vitro method has been developed utilizing phytohemagglutinin (PHA) activated lymphocytes obtained from human tonsils and adenoids which permit the accumulation of multi-liter quantities of cell-free supernatants containing lymphotoxin and other lymphocyte effector molecules (LEM). An enriched media is employed which contains a large molecular weight, heat stable bovine serum fraction which supports lymphoid cell activation and levels of LEM secretion equal to that of cultures maintained in medium supplemented with whole serum. Elimination of whole serum from the media greatly reduces overall protein concentrations and facilitates concentration and purification studies. Various technical aspects of these cultures have been examined, i.e.: 1) cell concentration, 2) kinetics of LT production over a ten-day period, 3) mitogen dosage, and 4) types of media. Supernatants can be harvested repeatedly from a single culture over the ten day period, thus doubling the yield of LEM collected from a single culture.", "contents": "Lymphocyte effector molecules: an vitro production method for obtaining liter volumes of supernatants from mitogen-stimulated human lymphocytes. An in vitro method has been developed utilizing phytohemagglutinin (PHA) activated lymphocytes obtained from human tonsils and adenoids which permit the accumulation of multi-liter quantities of cell-free supernatants containing lymphotoxin and other lymphocyte effector molecules (LEM). An enriched media is employed which contains a large molecular weight, heat stable bovine serum fraction which supports lymphoid cell activation and levels of LEM secretion equal to that of cultures maintained in medium supplemented with whole serum. Elimination of whole serum from the media greatly reduces overall protein concentrations and facilitates concentration and purification studies. Various technical aspects of these cultures have been examined, i.e.: 1) cell concentration, 2) kinetics of LT production over a ten-day period, 3) mitogen dosage, and 4) types of media. Supernatants can be harvested repeatedly from a single culture over the ten day period, thus doubling the yield of LEM collected from a single culture."}
{"id": "PMID:1084377", "title": "Cellular basis of interferon formation and hyporeactivity after exposure to bacterial lipopolysaccharide.", "content": "Endotoxin apparently induces interferon in only a few types of cell, yet it produces strong hyporesponsiveness to a large number of agents. After incubation for 24 hr with endotoxin in vitro, tissue cultures of thymus, spleen, mesenteric lymph nodes, and to a lesser extent, liver and lung produced interferon. Lymphoid tissues of bone marrow-derived (B-) cells (sacculus rotundus, appendix, and Peyer's patches) and kidney did not produce interferon. Adherent spleen cells produced more interferon than nonadherent cells. Purification of spleen cells on a bovine serum albumin gradient showed that light, DNA-synthesizing cells made interferon in response to endotoxin or polyriboinosinic-polyribocytidylic acid. Mouse spleen cells produced a \"late\" interferon 24-48 hr after exposure to endotoxin, which, in contrast to \"early\" interferon (produced at 0-24 hr) from spleen and other tissues, is stable at 56 C for 60 min. It is suggested that this late interferon represents a \"B-cell\" interferon. Hyporeactivity was produced in vitro by endotoxin only in tissues that make endotoxin-induced interferon, a fact consistent with the theory that the interferon-inducing mechanism must be initiated before hyporeactivity results. The fact that endotoxin has been found to act on a fairly large range of cells (i.e., macrophages, thymus-derived cells, and probably B-lymphocytes) explains its ability to produce broad hyporesponsiveness.", "contents": "Cellular basis of interferon formation and hyporeactivity after exposure to bacterial lipopolysaccharide. Endotoxin apparently induces interferon in only a few types of cell, yet it produces strong hyporesponsiveness to a large number of agents. After incubation for 24 hr with endotoxin in vitro, tissue cultures of thymus, spleen, mesenteric lymph nodes, and to a lesser extent, liver and lung produced interferon. Lymphoid tissues of bone marrow-derived (B-) cells (sacculus rotundus, appendix, and Peyer's patches) and kidney did not produce interferon. Adherent spleen cells produced more interferon than nonadherent cells. Purification of spleen cells on a bovine serum albumin gradient showed that light, DNA-synthesizing cells made interferon in response to endotoxin or polyriboinosinic-polyribocytidylic acid. Mouse spleen cells produced a \"late\" interferon 24-48 hr after exposure to endotoxin, which, in contrast to \"early\" interferon (produced at 0-24 hr) from spleen and other tissues, is stable at 56 C for 60 min. It is suggested that this late interferon represents a \"B-cell\" interferon. Hyporeactivity was produced in vitro by endotoxin only in tissues that make endotoxin-induced interferon, a fact consistent with the theory that the interferon-inducing mechanism must be initiated before hyporeactivity results. The fact that endotoxin has been found to act on a fairly large range of cells (i.e., macrophages, thymus-derived cells, and probably B-lymphocytes) explains its ability to produce broad hyporesponsiveness."}
{"id": "PMID:1084378", "title": "Enhanced expression of histocompatibility antigens of lymphoid cells in mice treated with interferon.", "content": "Treatment of young and mature mice with potent preparations of mouse interferon results in a marked enhancement of the expression of histocompatibility antigens on the surface of thymocytes and splenic lymphocytes, as measured by an enhanced absorption of alloantiserum. These modifications in the expression of cell surface antigens may reflect an effect of interferon on lymphocyte naturation and may be relevant to theffect of interferon on lymphocyte function.", "contents": "Enhanced expression of histocompatibility antigens of lymphoid cells in mice treated with interferon. Treatment of young and mature mice with potent preparations of mouse interferon results in a marked enhancement of the expression of histocompatibility antigens on the surface of thymocytes and splenic lymphocytes, as measured by an enhanced absorption of alloantiserum. These modifications in the expression of cell surface antigens may reflect an effect of interferon on lymphocyte naturation and may be relevant to theffect of interferon on lymphocyte function."}
{"id": "PMID:1084379", "title": "Leukocyte colony-stimulating factor and inhibitor activity.", "content": "Native and density gradient separated rat peritoneal exudate cells were evaluated for both their capacity to stimulate granulocyte colony formation and their ability to inhibit colony growth in vitro. Granulocyte colony-stimulating factor (CSF) was primarily elaborated by monocytes and macrophages; neutrophils showed only minimal stimulatory activity. In contrast, freeze-thawed extracts of both monocyte-macrophage and granulocyte fractions were markedly inhibitory to colony growth using a standard L-cell-derived CSF. Characterization of the inhibitory material indicated it to be a low molecular weight, nonlipid, heat-stable substance. In addition to peritoneal leukocytes, other tissues including lymph node, thymic and splenic lymphocytes, liver, kidney, and skeletal muscle also contained inhibitory materials. These observations suggest that granulopoiesis, as assessed by in vitro culture techniques, is not influenced solely by a simple feedback loop; rather, leukocytic elements, as well as other tissues, appear to contain and release both stimulatory and inhibitory factors.", "contents": "Leukocyte colony-stimulating factor and inhibitor activity. Native and density gradient separated rat peritoneal exudate cells were evaluated for both their capacity to stimulate granulocyte colony formation and their ability to inhibit colony growth in vitro. Granulocyte colony-stimulating factor (CSF) was primarily elaborated by monocytes and macrophages; neutrophils showed only minimal stimulatory activity. In contrast, freeze-thawed extracts of both monocyte-macrophage and granulocyte fractions were markedly inhibitory to colony growth using a standard L-cell-derived CSF. Characterization of the inhibitory material indicated it to be a low molecular weight, nonlipid, heat-stable substance. In addition to peritoneal leukocytes, other tissues including lymph node, thymic and splenic lymphocytes, liver, kidney, and skeletal muscle also contained inhibitory materials. These observations suggest that granulopoiesis, as assessed by in vitro culture techniques, is not influenced solely by a simple feedback loop; rather, leukocytic elements, as well as other tissues, appear to contain and release both stimulatory and inhibitory factors."}
{"id": "PMID:1084380", "title": "The influence of selective thrombocytopenia on nephrotoxic nephritis.", "content": "Anticoagulation with agents that interfere with fibrin formation inhibit the development of the autologous phase of nephrotoxic nephritis (NTN). Platelet participation in the nephritic process has been suggested but not proved, therefore, the influence of selective thrombocytopenia on the autologous phase in rabbits was evaluated. NTN was produced with goat antirabbit glomerular basement membrane antiserum. Thrombocytopenia was induced with goat antirabbit platelet antiserum 24 hours prior to the onset of nephritis. Platelet accumulation within the nephritic kidney was quantitated using chromium labeled platelets. Thrombocytopenia has no inhibitory effect on the development of the autologous phase of NTN in rabbits. There was no platelet accumulation within the nephritic kidney in the presence of thrombocytopenia. Pharmacologic inhibition of platelet aggregation may be of no benefit in glomerulonephritis produced by a fixed antigen-antibody reaction within the glomerular capillary wall.", "contents": "The influence of selective thrombocytopenia on nephrotoxic nephritis. Anticoagulation with agents that interfere with fibrin formation inhibit the development of the autologous phase of nephrotoxic nephritis (NTN). Platelet participation in the nephritic process has been suggested but not proved, therefore, the influence of selective thrombocytopenia on the autologous phase in rabbits was evaluated. NTN was produced with goat antirabbit glomerular basement membrane antiserum. Thrombocytopenia was induced with goat antirabbit platelet antiserum 24 hours prior to the onset of nephritis. Platelet accumulation within the nephritic kidney was quantitated using chromium labeled platelets. Thrombocytopenia has no inhibitory effect on the development of the autologous phase of NTN in rabbits. There was no platelet accumulation within the nephritic kidney in the presence of thrombocytopenia. Pharmacologic inhibition of platelet aggregation may be of no benefit in glomerulonephritis produced by a fixed antigen-antibody reaction within the glomerular capillary wall."}
{"id": "PMID:1084381", "title": "Determination of alpha1-antitrypsin phenotypes by isoelectric focusing in polyacrylamide gels.", "content": "A method for alpha1-antitrypsin phenotyping is described that uses isoelectric focusing in a pH gradient 3.5 to 5 with polyacrylamide as supporting medium. The method is fast, reproducible, and reliable and offers some advantages over the currently used starch-gel electrophoresis.", "contents": "Determination of alpha1-antitrypsin phenotypes by isoelectric focusing in polyacrylamide gels. A method for alpha1-antitrypsin phenotyping is described that uses isoelectric focusing in a pH gradient 3.5 to 5 with polyacrylamide as supporting medium. The method is fast, reproducible, and reliable and offers some advantages over the currently used starch-gel electrophoresis."}
{"id": "PMID:1084399", "title": "Ly and Ia antigen phenotypes of T cells involved in delayed-type hypersensitivity and in suppression.", "content": "The Ly and Ia phenotypes of T lymphocytes involved in different functions were characterized by the use of specific antisera. T cells responsible for delayed-type hypersensitivity (DTH) and for helper functions were found to be Ly-1+,2- in contrast to cytotoxic T cells and T cells responsible for suppression of antibody responses which were Ly-1-,2+. Unlike some primed helper cells, T cells involved in DTH were Ia-. Suppressor cells in the system were Ia+.", "contents": "Ly and Ia antigen phenotypes of T cells involved in delayed-type hypersensitivity and in suppression. The Ly and Ia phenotypes of T lymphocytes involved in different functions were characterized by the use of specific antisera. T cells responsible for delayed-type hypersensitivity (DTH) and for helper functions were found to be Ly-1+,2- in contrast to cytotoxic T cells and T cells responsible for suppression of antibody responses which were Ly-1-,2+. Unlike some primed helper cells, T cells involved in DTH were Ia-. Suppressor cells in the system were Ia+."}
{"id": "PMID:1084400", "title": "Inhibition of antibody-dependent cellular cytotoxicity and immunoglobulin synthesis by an antiserum prepared against a human B-cell Ia-like molecule.", "content": "Rabbit antisera to the human B-cell-specific antigen complex, p23,30, was used to define further the functional heterogeneity of isolated human lymphocyte subpopulations. Specific depletion of p23,30-bearing cells from Ig-negative cell populations and Ig-negative, E rosette-negative (Null) populations by either complement-mediated lysis or by physical separation on goat antirabbit Fab immunoabsorbent columns, eliminates the antibody-dependent cellular cytotoxic (ADCC) function. Furthermore, binding of anti-p23,30 serum to the effector cell surface inhibits ADCC but does not interfere with EA rosette formation. Apparently p23,30 represents a cell surface site which is distinct from the Fc receptor but which is important in the triggering of ADCC. In addition, depletion of p23,30-bearing cells from unfractionated cell populations, Ig-positive B-cell populations and Ig-negative, E rosette-negative (Null) populations eliminates the capacity of these populations to secrete immunoglobulin during subsequent culturing. Thus both the Ig-secreting cells and the ADCC effector cells within the Ig-negative, E rosette-negative (Null) population reside in the same population of cells which bears the p23,30 antigen.", "contents": "Inhibition of antibody-dependent cellular cytotoxicity and immunoglobulin synthesis by an antiserum prepared against a human B-cell Ia-like molecule. Rabbit antisera to the human B-cell-specific antigen complex, p23,30, was used to define further the functional heterogeneity of isolated human lymphocyte subpopulations. Specific depletion of p23,30-bearing cells from Ig-negative cell populations and Ig-negative, E rosette-negative (Null) populations by either complement-mediated lysis or by physical separation on goat antirabbit Fab immunoabsorbent columns, eliminates the antibody-dependent cellular cytotoxic (ADCC) function. Furthermore, binding of anti-p23,30 serum to the effector cell surface inhibits ADCC but does not interfere with EA rosette formation. Apparently p23,30 represents a cell surface site which is distinct from the Fc receptor but which is important in the triggering of ADCC. In addition, depletion of p23,30-bearing cells from unfractionated cell populations, Ig-positive B-cell populations and Ig-negative, E rosette-negative (Null) populations eliminates the capacity of these populations to secrete immunoglobulin during subsequent culturing. Thus both the Ig-secreting cells and the ADCC effector cells within the Ig-negative, E rosette-negative (Null) population reside in the same population of cells which bears the p23,30 antigen."}
{"id": "PMID:1084401", "title": "Antibody response of C3H in equilibrium (CKB X CWB)F1 tetraparental mice to poly-L(Tyr,Glu)-poly-D,L-Ala-poly-L-Lys immunization.", "content": "To test whether the antigen-specific stimulation of low responder-genotype B cells in tetraparental mice is due to a histoincompatibility reaction (allogeneic effect) against these B cells, tetraparental mice were constructed (a) between an Ir-1A low responder to the antigen poly-L(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys. [(T,G)-A--L] and an Ir-1A F1 high responder and (b) between two histoincompatible Ir-lA low responders. In the first case the F1 high responder embryo shares the whole of the H-2 complex, including Ir, with the low responder embryo.", "contents": "Antibody response of C3H in equilibrium (CKB X CWB)F1 tetraparental mice to poly-L(Tyr,Glu)-poly-D,L-Ala-poly-L-Lys immunization. To test whether the antigen-specific stimulation of low responder-genotype B cells in tetraparental mice is due to a histoincompatibility reaction (allogeneic effect) against these B cells, tetraparental mice were constructed (a) between an Ir-1A low responder to the antigen poly-L(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys. [(T,G)-A--L] and an Ir-1A F1 high responder and (b) between two histoincompatible Ir-lA low responders. In the first case the F1 high responder embryo shares the whole of the H-2 complex, including Ir, with the low responder embryo."}
{"id": "PMID:1084402", "title": "The modulation of lymphocyte functions by molecules secreted by macrophages. II. Conditions leading to increased secretion.", "content": "Cultures of peritoneal exudate cells rich in macrophages were studied for the secretion of lymphostimulatory molecules. Two conditions produced increased secretion: (a) addition to the cultures of various agents that readily interacted with macrophages, such as latex particles, antibody-coated red cells, endotoxin, Listeria organisms, or Be salt; or (b) addition of activated lymphocytes. In the first case the increased activity was found during the first 24 or 48 h after uptake of the stimuli. Increased activity was found in normal or peptone-stimulated macrophages but not in macrophages after injection of endotoxin or thioglycollate. The addition of T lymphocytes from Listeria-infected mice to macrophage cultures increased greatly the activities. This increase was also produced by addition to antigen-primed T cells together with antigen. The lymphocytes by themselves did not secrete active factors. The lymphostimulatory activities were tested on thymocyte DNA synthesis and on antibody formation in vitro. The latter assay was done on spleen cells from immunized mice where one striking effect was the stimulation of differentiation to antibody-secreting cells. Some dissociation of both activities (thymocyte DNA synthesis and B-cell differentiation) was observed with selected culture fluids.", "contents": "The modulation of lymphocyte functions by molecules secreted by macrophages. II. Conditions leading to increased secretion. Cultures of peritoneal exudate cells rich in macrophages were studied for the secretion of lymphostimulatory molecules. Two conditions produced increased secretion: (a) addition to the cultures of various agents that readily interacted with macrophages, such as latex particles, antibody-coated red cells, endotoxin, Listeria organisms, or Be salt; or (b) addition of activated lymphocytes. In the first case the increased activity was found during the first 24 or 48 h after uptake of the stimuli. Increased activity was found in normal or peptone-stimulated macrophages but not in macrophages after injection of endotoxin or thioglycollate. The addition of T lymphocytes from Listeria-infected mice to macrophage cultures increased greatly the activities. This increase was also produced by addition to antigen-primed T cells together with antigen. The lymphocytes by themselves did not secrete active factors. The lymphostimulatory activities were tested on thymocyte DNA synthesis and on antibody formation in vitro. The latter assay was done on spleen cells from immunized mice where one striking effect was the stimulation of differentiation to antibody-secreting cells. Some dissociation of both activities (thymocyte DNA synthesis and B-cell differentiation) was observed with selected culture fluids."}
{"id": "PMID:1084403", "title": "Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. III. Dual gene control of the T-cell-mediated suppression of the antibody response.", "content": "The antigen-specific suppressive T-cell factor of mice, which had previously been shown to be an I region gene product, could effectively suppress the in vitro secondary antibody response of spleen cells from syngeneic or H-2 compatible mouse strains but not that of H-2 incompatible strains. The identities among genes in the left side half (K, I-A, and I-B) of the H-2 complex between the donor and recipient strains were found to be both necessary and sufficient for the induction of suppression. This suggests that the acceptor site for the suppressive T-cell factor is also determined by the gene present in the left side half of the H-2 complex. The cell type which expresses the acceptor site was found to be a subset of T cell. In general, the suppressive T-cell factor obtained from F1 mice could suppress the responses of both parental strains, and the parental factors could suppress the response of F1 mice. The results indicate that both suppressor and acceptor molecules are codominantly expressed on F1 T cells. There were found two types of defects in the expression of suppressor and acceptor molecules among mouse strains: A/J mice could not produce the suppressive T-cell factor despite that they could accept the factor produced by other H-2 compatible mouse strains. In contrast, all the B10 congeneic lines could produce the T-cell factor, but could not accept the factor produced by syngeneic and H-2 compatible non-B10 congeneic lines. The F1 hybrid of A/J and B10. A could both produce and accept the T-cell factor, and thus the expressions of suppressor and acceptor molecules were found to be dominant traits. These results indicate that the antigen-specific T-cell-mediated suppression is regulated by at least two genes both present in the H-2 complex, and that the complementation of these two genes is required for the induction of suppression.", "contents": "Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. III. Dual gene control of the T-cell-mediated suppression of the antibody response. The antigen-specific suppressive T-cell factor of mice, which had previously been shown to be an I region gene product, could effectively suppress the in vitro secondary antibody response of spleen cells from syngeneic or H-2 compatible mouse strains but not that of H-2 incompatible strains. The identities among genes in the left side half (K, I-A, and I-B) of the H-2 complex between the donor and recipient strains were found to be both necessary and sufficient for the induction of suppression. This suggests that the acceptor site for the suppressive T-cell factor is also determined by the gene present in the left side half of the H-2 complex. The cell type which expresses the acceptor site was found to be a subset of T cell. In general, the suppressive T-cell factor obtained from F1 mice could suppress the responses of both parental strains, and the parental factors could suppress the response of F1 mice. The results indicate that both suppressor and acceptor molecules are codominantly expressed on F1 T cells. There were found two types of defects in the expression of suppressor and acceptor molecules among mouse strains: A/J mice could not produce the suppressive T-cell factor despite that they could accept the factor produced by other H-2 compatible mouse strains. In contrast, all the B10 congeneic lines could produce the T-cell factor, but could not accept the factor produced by syngeneic and H-2 compatible non-B10 congeneic lines. The F1 hybrid of A/J and B10. A could both produce and accept the T-cell factor, and thus the expressions of suppressor and acceptor molecules were found to be dominant traits. These results indicate that the antigen-specific T-cell-mediated suppression is regulated by at least two genes both present in the H-2 complex, and that the complementation of these two genes is required for the induction of suppression."}
{"id": "PMID:1084404", "title": "Histocompatibility antigens and genetic control of the immune response in guinea pigs. III. Specific inhibition of antigen-induced lymphocyte proliferation by strain-specific anti-idiotypic antibodies.", "content": "The in vitro T-cell proliferation induced by penicilloylated bovine IgG (BPO-BGG) in sensitized strain 2 and strain 13 guinea pigs could be specifically blocked by strain-specific antisera presumably directed against cell membrane-associated immunoglobulin idiotypes. The anti-idiotypic antisera were prepared in strain 2 and strain 13 guinea pigs against immunoadsorbent purified anti-BPO-BGG antibodies which had been raised in strain 2 and strain 13 animals. Strain 13 antistrain 13 anti-BPO-BGG (a strain 13 BPO-BGG) suppressed the in vitro BPO-BGG response of cells from immunized strain 13 animals but did not inhibit the response of cells from immune strain 2 animals. Conversely, the corresponding antiserum raised in a strain 2 combination (a strain 2 BPO-BGG) only inhibited the in vitro BPO-BGG response of strain 2 cells. Furthermore, the inhibitory activity of the antisera could only be absorbed by immune cells from the syngeneic strain. The activity of the a strain 13 BPO-BGG serum was highly specific; the inhibitory activity could only be absorbed by BPO-BGG-sensitive strain 13 cells. The inhibitory activity of the anti-idiotypic sera was predominantly associated with the 19S fraction. The data suggest that immune cells and in particular T lymphocytes from strain 2 and strain 13 guinea pigs possess strain-specific recognition structures from BPO-BGG with the same idiotypes as the corresponding strain-specific immunoglobulins. Furthermore, the production of such inhibitory anti-idiotypic sera was restricted to syngeneic combinations, which suggests a potential role of autoanti-idiotypic antibodies in the regulation of the immune response. The anti-idiotypic antisera used here are apparently directed against gene products not associated with the strain 2 or strain 13 major histocompatibility complex.", "contents": "Histocompatibility antigens and genetic control of the immune response in guinea pigs. III. Specific inhibition of antigen-induced lymphocyte proliferation by strain-specific anti-idiotypic antibodies. The in vitro T-cell proliferation induced by penicilloylated bovine IgG (BPO-BGG) in sensitized strain 2 and strain 13 guinea pigs could be specifically blocked by strain-specific antisera presumably directed against cell membrane-associated immunoglobulin idiotypes. The anti-idiotypic antisera were prepared in strain 2 and strain 13 guinea pigs against immunoadsorbent purified anti-BPO-BGG antibodies which had been raised in strain 2 and strain 13 animals. Strain 13 antistrain 13 anti-BPO-BGG (a strain 13 BPO-BGG) suppressed the in vitro BPO-BGG response of cells from immunized strain 13 animals but did not inhibit the response of cells from immune strain 2 animals. Conversely, the corresponding antiserum raised in a strain 2 combination (a strain 2 BPO-BGG) only inhibited the in vitro BPO-BGG response of strain 2 cells. Furthermore, the inhibitory activity of the antisera could only be absorbed by immune cells from the syngeneic strain. The activity of the a strain 13 BPO-BGG serum was highly specific; the inhibitory activity could only be absorbed by BPO-BGG-sensitive strain 13 cells. The inhibitory activity of the anti-idiotypic sera was predominantly associated with the 19S fraction. The data suggest that immune cells and in particular T lymphocytes from strain 2 and strain 13 guinea pigs possess strain-specific recognition structures from BPO-BGG with the same idiotypes as the corresponding strain-specific immunoglobulins. Furthermore, the production of such inhibitory anti-idiotypic sera was restricted to syngeneic combinations, which suggests a potential role of autoanti-idiotypic antibodies in the regulation of the immune response. The anti-idiotypic antisera used here are apparently directed against gene products not associated with the strain 2 or strain 13 major histocompatibility complex."}
{"id": "PMID:1084405", "title": "Requirement for non-T cells in the generation of cytotoxic T lymphocytes in vitro. I. Use of nude mice as source of non-T cells.", "content": "The ability of small numbers of LN cells to produce cytotoxic lymphocytes on in vitro culture with allogeneic stimulator cells is greatly augmented by the addition of spleen cells from athymic nude mice. The possibility that the synergism is a result of improved culture conditions or a \"feeder effect\" is excluded. All cytotoxic cells found in these cultures are shown to be T cells and to arise from precursors contained in the LN-cell component. The nude spleen cell component appears to be providing a required non-T cell which has been lost from the LN component through dilution. Synergism between the two components can occur whether they are syngeneic or allogeneic provided that both can recognize the same alloantigens in the stimulator population.", "contents": "Requirement for non-T cells in the generation of cytotoxic T lymphocytes in vitro. I. Use of nude mice as source of non-T cells. The ability of small numbers of LN cells to produce cytotoxic lymphocytes on in vitro culture with allogeneic stimulator cells is greatly augmented by the addition of spleen cells from athymic nude mice. The possibility that the synergism is a result of improved culture conditions or a \"feeder effect\" is excluded. All cytotoxic cells found in these cultures are shown to be T cells and to arise from precursors contained in the LN-cell component. The nude spleen cell component appears to be providing a required non-T cell which has been lost from the LN component through dilution. Synergism between the two components can occur whether they are syngeneic or allogeneic provided that both can recognize the same alloantigens in the stimulator population."}
{"id": "PMID:1084406", "title": "Genetic regulation of the antibody response to H-2Db alloantigens in mice. II. Tolerance to non-H-2 determinants abolishes the antibody response to H-2Db in B10.A(5R) mice.", "content": "B10.A(5R) mice (H-2i5), immunized with spleen cells from congenic B10 mice (H-2b), responded to alloantigens of the H-2Db region by producing antibodies of only IgM type. In contrast, they produced both IgM and IgG antibodies when immunized with noncongenic H-2b cells that carry other foreign cell surface antigens (non-H-2) in addition to H-2Db. A hypothesis was proposed comparing the H-2Db antigen on a congenic cell to a hapten on a nonimmunogenic carrier which fails to induce T-cell helper function responsible for the switch from IgM to IgG secretion in B cells. Data presented here confirmed this hypothesis. 5R mice rendered tolerant to the relevant non-H-2 antigens were unable to mount the anti-H-2Db IgG response in a noncongenic immunization. Tolerance induction did not lead to abrogation of the T-cell mediated cytotoxicity.", "contents": "Genetic regulation of the antibody response to H-2Db alloantigens in mice. II. Tolerance to non-H-2 determinants abolishes the antibody response to H-2Db in B10.A(5R) mice. B10.A(5R) mice (H-2i5), immunized with spleen cells from congenic B10 mice (H-2b), responded to alloantigens of the H-2Db region by producing antibodies of only IgM type. In contrast, they produced both IgM and IgG antibodies when immunized with noncongenic H-2b cells that carry other foreign cell surface antigens (non-H-2) in addition to H-2Db. A hypothesis was proposed comparing the H-2Db antigen on a congenic cell to a hapten on a nonimmunogenic carrier which fails to induce T-cell helper function responsible for the switch from IgM to IgG secretion in B cells. Data presented here confirmed this hypothesis. 5R mice rendered tolerant to the relevant non-H-2 antigens were unable to mount the anti-H-2Db IgG response in a noncongenic immunization. Tolerance induction did not lead to abrogation of the T-cell mediated cytotoxicity."}
{"id": "PMID:1084407", "title": "Genetic control of specific immune suppression. IV. Responsiveness to the random copolymer L-glutamic acid50-L-tyrosine50 induced in BALB/c mice by cyclophosphamide.", "content": "Previous reports from our laboratory have demonstrated the stimulation of specific suppressor T cells in genetic nonresponder mice after immunization with the terpolymer of L- glutamic acid, L-alanine, and L-tyrosine (GAT) (1,2) and with the copolymer of L-glutamic acid and L-tyrosine (GT) (3-5). These findings raise two important questions: (a) do the specific suppressor T cells inhibit an antibody response which would otherwise develop in nonresponder mice; and, (b) can specific helper T cells inhibit an antibody response which would otherwise develop in nonresponder mice; and, (b) can specific helper T-cell activity be detected in these animals. Responsiveness appears to be completely dominant over suppression in (responder x suppressor)F(1) hybrids, therefore, we have been unable to detect suppressor cells in these hybrids after conventional immunization with GAT (2). However , using special conditions of antigen administration, GAT helper activity could be demonstrated in nonresponder DBA/1 (\"suppressor\") mice. Thus, GAT-specific helper activity was not detected in these nonresponder animals after immunization with GAT irrespective of the adjuvant used, but could be stimulated by macrophage-bound GAT or by GAT complexed with methylated bovine serum albumin GAT-MBSA (6). In the current report we have taken advantage of the fact that suppressor T-cell activity is more sensitive to cyclophosphamide treatment than T-cell helper activity (7) to demonstrate the presence of GT-specific helper activity in \"nonresponder\" BALB/c mice. We describe: (a) the dose of cyclophosphamide and conditions of treatment which inhibits the well-documented stimulation of specific suppressor T cells in BALB/c mice injected with GT previous to immunization with GT-MBSA, and (b) the ability of cyclophosphamide to permit the development of primary PFC responses to GT in these \"nonresponder\" mice. These cyclophosphamide-induced responses are not characterized by the high levels of antibody detected in genetic responder animals.", "contents": "Genetic control of specific immune suppression. IV. Responsiveness to the random copolymer L-glutamic acid50-L-tyrosine50 induced in BALB/c mice by cyclophosphamide. Previous reports from our laboratory have demonstrated the stimulation of specific suppressor T cells in genetic nonresponder mice after immunization with the terpolymer of L- glutamic acid, L-alanine, and L-tyrosine (GAT) (1,2) and with the copolymer of L-glutamic acid and L-tyrosine (GT) (3-5). These findings raise two important questions: (a) do the specific suppressor T cells inhibit an antibody response which would otherwise develop in nonresponder mice; and, (b) can specific helper T cells inhibit an antibody response which would otherwise develop in nonresponder mice; and, (b) can specific helper T-cell activity be detected in these animals. Responsiveness appears to be completely dominant over suppression in (responder x suppressor)F(1) hybrids, therefore, we have been unable to detect suppressor cells in these hybrids after conventional immunization with GAT (2). However , using special conditions of antigen administration, GAT helper activity could be demonstrated in nonresponder DBA/1 (\"suppressor\") mice. Thus, GAT-specific helper activity was not detected in these nonresponder animals after immunization with GAT irrespective of the adjuvant used, but could be stimulated by macrophage-bound GAT or by GAT complexed with methylated bovine serum albumin GAT-MBSA (6). In the current report we have taken advantage of the fact that suppressor T-cell activity is more sensitive to cyclophosphamide treatment than T-cell helper activity (7) to demonstrate the presence of GT-specific helper activity in \"nonresponder\" BALB/c mice. We describe: (a) the dose of cyclophosphamide and conditions of treatment which inhibits the well-documented stimulation of specific suppressor T cells in BALB/c mice injected with GT previous to immunization with GT-MBSA, and (b) the ability of cyclophosphamide to permit the development of primary PFC responses to GT in these \"nonresponder\" mice. These cyclophosphamide-induced responses are not characterized by the high levels of antibody detected in genetic responder animals."}
{"id": "PMID:1084408", "title": "Association between Ia antigens and the Fc receptors of certain T lymphocytes.", "content": "The Fc receptors of thymic and splenic T lymphocytes were detected using indirect immunofluorescence and soluble antigen-antibody complexes. 10-20% of thymocytes and 40-50% of Thy-1-positive splenic lymphocytes bound antigen-complexed Ig. The binding to thymocytes was partially inhibited (45-74%) by antibodies against antigens determined by the I region of the H-2 complex, but not by antibodies against K- or D-region antigens or Thy-1 antigen. The inhibition did not require the Fc portion of the inhibiting antibody. These results provide evidence that Ia antigens and the Fc receptors of some T lymphocytes are associated, and that the populations of T cells which bear these moieties at least partially overlap.", "contents": "Association between Ia antigens and the Fc receptors of certain T lymphocytes. The Fc receptors of thymic and splenic T lymphocytes were detected using indirect immunofluorescence and soluble antigen-antibody complexes. 10-20% of thymocytes and 40-50% of Thy-1-positive splenic lymphocytes bound antigen-complexed Ig. The binding to thymocytes was partially inhibited (45-74%) by antibodies against antigens determined by the I region of the H-2 complex, but not by antibodies against K- or D-region antigens or Thy-1 antigen. The inhibition did not require the Fc portion of the inhibiting antibody. These results provide evidence that Ia antigens and the Fc receptors of some T lymphocytes are associated, and that the populations of T cells which bear these moieties at least partially overlap."}
{"id": "PMID:1084409", "title": "Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B-cell tolerance.", "content": "The relative susceptibility of neonatal and adult murine splenocytes to induction of B-cell tolerance was studied in vitro. Adult cells required approximately 1,000-fold more trinitrophenyl-human gamma globulin to be rendered tolerant than did cells from 9- to 12-day-old neonates. The potential effects of suppressor T cells were excluded by pretreating the cultured B cells with anti-Thy-1 and C' and the helper T cells with anti-Ly-2.2 and C'. The possible role of cell surface immunoglobulin isotypes in contributing to this observed difference is discussed.", "contents": "Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B-cell tolerance. The relative susceptibility of neonatal and adult murine splenocytes to induction of B-cell tolerance was studied in vitro. Adult cells required approximately 1,000-fold more trinitrophenyl-human gamma globulin to be rendered tolerant than did cells from 9- to 12-day-old neonates. The potential effects of suppressor T cells were excluded by pretreating the cultured B cells with anti-Thy-1 and C' and the helper T cells with anti-Ly-2.2 and C'. The possible role of cell surface immunoglobulin isotypes in contributing to this observed difference is discussed."}
{"id": "PMID:1084410", "title": "Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro.", "content": "A new method for the isolation of specific immunocompetent lymphocytes has been described in which lymphocyte populations are exposed to fluoresceinated antigens (FLAGs) in vivo or in vitro, and the FLAG-binding cells retained on antifluorescein affinity columns. Specific cells are then eluted with an unrelated FL-labeled protein and shown to be fully immunocompetent. This methodology has been applied successfully in diverse antigenic systems including polymerized flagellin and TNP-specific B cells and alloantigen-reactive cytotoxic T lymphocytes. The method is rapid, inexpensive (requiring only antifluorescein beads), and can be applied to any antigens (or antibodies) in which a fluorescein group can be introduced.", "contents": "Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro. A new method for the isolation of specific immunocompetent lymphocytes has been described in which lymphocyte populations are exposed to fluoresceinated antigens (FLAGs) in vivo or in vitro, and the FLAG-binding cells retained on antifluorescein affinity columns. Specific cells are then eluted with an unrelated FL-labeled protein and shown to be fully immunocompetent. This methodology has been applied successfully in diverse antigenic systems including polymerized flagellin and TNP-specific B cells and alloantigen-reactive cytotoxic T lymphocytes. The method is rapid, inexpensive (requiring only antifluorescein beads), and can be applied to any antigens (or antibodies) in which a fluorescein group can be introduced."}
{"id": "PMID:1084411", "title": "Ontogeny of B cells in the chicken. I. Sequential development of clonal diversity in the bursa.", "content": "The initial development and distribution of lymphocytes expressing surface IgM (sIgM) and of specific antigen-binding cells (ABC) were studied in the chicken in an attempt to gain information on the process by which B-cell diversity is generated. The antigens used were sheep erythrocytes (SE), keyhole limpet hemocyanin (KLH), and poly-L(Tyr, Glu)poly-D,L-Ala-poly-L-Lys (TGAL). The results indicate that generation of the total sIgM-positive population begins in the bursa and that specific clones of ABC develop in a fixed sequential pattern which is not influenced by either deprivation of or deliberate exposure to exogenous antigens. Cells bearing sIgM by immunofluorescence (IgM-positive cells) were detected first in the bursa on the 12th day of incubation, KLH-ABC and TGAL-ABC by the 16th day, and SE-ABC on the 18th day. The doubling time of the sIgM-positive population of bursal cells was determined to be approximately 10 h before significant antigen-independent seeding to the spleen began a few days before hatching. Clonal expansion of SE-ABC in the bursa also appeared to be antigen independent as was the initial development of SE-ABC in the blood and spleen which ceased abruptly after bursectomy at hatching. Specific ABC were observed to develop in multiple bursal follicles as small foci of ABC among the much larger total population of sIgM-positive cells within an individual follicle. Intravenously infused SE-ABC homed to the embryonic spleen but not to the bursa. The results are interpreted as favoring a hypothetical model in which individual stem cells give rise to multiple clones of B cells by a predetermined pattern of sequential expression of variable region genes.", "contents": "Ontogeny of B cells in the chicken. I. Sequential development of clonal diversity in the bursa. The initial development and distribution of lymphocytes expressing surface IgM (sIgM) and of specific antigen-binding cells (ABC) were studied in the chicken in an attempt to gain information on the process by which B-cell diversity is generated. The antigens used were sheep erythrocytes (SE), keyhole limpet hemocyanin (KLH), and poly-L(Tyr, Glu)poly-D,L-Ala-poly-L-Lys (TGAL). The results indicate that generation of the total sIgM-positive population begins in the bursa and that specific clones of ABC develop in a fixed sequential pattern which is not influenced by either deprivation of or deliberate exposure to exogenous antigens. Cells bearing sIgM by immunofluorescence (IgM-positive cells) were detected first in the bursa on the 12th day of incubation, KLH-ABC and TGAL-ABC by the 16th day, and SE-ABC on the 18th day. The doubling time of the sIgM-positive population of bursal cells was determined to be approximately 10 h before significant antigen-independent seeding to the spleen began a few days before hatching. Clonal expansion of SE-ABC in the bursa also appeared to be antigen independent as was the initial development of SE-ABC in the blood and spleen which ceased abruptly after bursectomy at hatching. Specific ABC were observed to develop in multiple bursal follicles as small foci of ABC among the much larger total population of sIgM-positive cells within an individual follicle. Intravenously infused SE-ABC homed to the embryonic spleen but not to the bursa. The results are interpreted as favoring a hypothetical model in which individual stem cells give rise to multiple clones of B cells by a predetermined pattern of sequential expression of variable region genes."}
{"id": "PMID:1084412", "title": "[Cytogenetic and statistical study of a group of patients treated with butazolidine (phenylbutazone)].", "content": "The authors studied a group of 16 patients suffering from rheumatic diseases, who were being treated with Butazolidine (Phenylbutazone). The control cases were divided into two groups: one consisted of six patients who were suffering from the same chronic rheumatic diseases, but who were being treated with other drugs; the other group consisted of 15 normal persons whose age and sex corresponded, more or less, with those of the group of patients treated with Butazolidine. A chromosomal examination of the lymphocytes of the peripheral blood was carried out on the 16 patients and the 21 persons not being treated with Butazolidine. A comparison of the karyotypes of all the individuals studied showed no significant difference in the number of breakages between the three groups. Statistical analysis confirmed that the differences in the percentages of chromosomal damage were not significant.", "contents": "[Cytogenetic and statistical study of a group of patients treated with butazolidine (phenylbutazone)]. The authors studied a group of 16 patients suffering from rheumatic diseases, who were being treated with Butazolidine (Phenylbutazone). The control cases were divided into two groups: one consisted of six patients who were suffering from the same chronic rheumatic diseases, but who were being treated with other drugs; the other group consisted of 15 normal persons whose age and sex corresponded, more or less, with those of the group of patients treated with Butazolidine. A chromosomal examination of the lymphocytes of the peripheral blood was carried out on the 16 patients and the 21 persons not being treated with Butazolidine. A comparison of the karyotypes of all the individuals studied showed no significant difference in the number of breakages between the three groups. Statistical analysis confirmed that the differences in the percentages of chromosomal damage were not significant."}
{"id": "PMID:1084413", "title": "Computerized tomography (the EMI Scanner): a comparison with pneumoencephalography and ventriculography.", "content": "Computerized tomography, using the EMI Scanner, allows the diagnosis of cerebral atrophy or hydrocephalus to be made with the same degree of accuracy as conventional neuroradiological methods. Ventricular measurements made on EMI scans have been compared with those from pneumoencephalograms and ventriculograms. A range of normal ventricular measurements for the EMI scan is suggested.", "contents": "Computerized tomography (the EMI Scanner): a comparison with pneumoencephalography and ventriculography. Computerized tomography, using the EMI Scanner, allows the diagnosis of cerebral atrophy or hydrocephalus to be made with the same degree of accuracy as conventional neuroradiological methods. Ventricular measurements made on EMI scans have been compared with those from pneumoencephalograms and ventriculograms. A range of normal ventricular measurements for the EMI scan is suggested."}
{"id": "PMID:1084415", "title": "Systemic candidiasis and endophthalmitis in neurosurgical patients. Report of three cases.", "content": "Three cases of systemic candidiasis in neurosurgical patients are presented. Two of the three patients also had endophthalmitis. All of the cases were treated with broad-spectrum antibiotics and glucocorticoids prior to the appearance of candidiasis. The authors stress the high susceptibility of neurosurgical patients to opportunistic infections of this type.", "contents": "Systemic candidiasis and endophthalmitis in neurosurgical patients. Report of three cases. Three cases of systemic candidiasis in neurosurgical patients are presented. Two of the three patients also had endophthalmitis. All of the cases were treated with broad-spectrum antibiotics and glucocorticoids prior to the appearance of candidiasis. The authors stress the high susceptibility of neurosurgical patients to opportunistic infections of this type."}
{"id": "PMID:1084417", "title": "Tubuloreticular structures and antinuclear antibodies in autoimmune and non-autoimmune diseases.", "content": "Tissues obtained at random from patients suffering from autoimmune and non-autoimmune diseases were studied for the presence of tubuloreticular structures (TRS). It could be demonstrated that the occurrence of TRS in renal tissue is not a specific characteristic of systemic lupus erythematosus whereas the presence of such structures in skin tissue might be suggestive for this disease. The serum of some of the patients could be studied for the presence of antinuclear antibodies (ANA). A statistically significant correlation was found between TRS and ANA in the group of patients with autoimmune diseases. The possibility is discussed that this correlation might favour the theory that viruses may be involved in the aetiology of autoimmune diseases, particularly of systemic lupus erythematosus.", "contents": "Tubuloreticular structures and antinuclear antibodies in autoimmune and non-autoimmune diseases. Tissues obtained at random from patients suffering from autoimmune and non-autoimmune diseases were studied for the presence of tubuloreticular structures (TRS). It could be demonstrated that the occurrence of TRS in renal tissue is not a specific characteristic of systemic lupus erythematosus whereas the presence of such structures in skin tissue might be suggestive for this disease. The serum of some of the patients could be studied for the presence of antinuclear antibodies (ANA). A statistically significant correlation was found between TRS and ANA in the group of patients with autoimmune diseases. The possibility is discussed that this correlation might favour the theory that viruses may be involved in the aetiology of autoimmune diseases, particularly of systemic lupus erythematosus."}
{"id": "PMID:1084418", "title": "Possible effect of maternal promethazine therapy on neonatal immunologic functions.", "content": "The effect of the administration of promethazine in the treatment of erythroblastosis fetalis was studied in four maternal-fetal pairs. The three infants exposed for a prolonged period of time had decreased neonatal number and function of T cells, and abnormal specific humoral immune responses. The possible role of promethazine in the induction of fetal immunoincompetence is discussed.", "contents": "Possible effect of maternal promethazine therapy on neonatal immunologic functions. The effect of the administration of promethazine in the treatment of erythroblastosis fetalis was studied in four maternal-fetal pairs. The three infants exposed for a prolonged period of time had decreased neonatal number and function of T cells, and abnormal specific humoral immune responses. The possible role of promethazine in the induction of fetal immunoincompetence is discussed."}
{"id": "PMID:1084419", "title": "Gingival hemorrhage related to aspirin ingestion. A case report.", "content": "A case report illustrating the clinical manifestations of platelet defects induced by ingestion of two aspirin tablets has been presented. The specific effects on platelets produced by aspirin ingestion have been summarized and the resultant hemostatic defect explained.", "contents": "Gingival hemorrhage related to aspirin ingestion. A case report. A case report illustrating the clinical manifestations of platelet defects induced by ingestion of two aspirin tablets has been presented. The specific effects on platelets produced by aspirin ingestion have been summarized and the resultant hemostatic defect explained."}
{"id": "PMID:1084420", "title": "Brain and peripheral metabolism of 5-hydroxytryptophan-14C following peripheral decarboxylase inhibition.", "content": "Following selective peripheral decarboxylase inhibition, a greater fraction of peripherally measured monoamine metabolites should derive from central nervous system monoamine metabolism. In rats pretreated with the peripheral decarboxylase inhibitor L-alpha-(3,4-dihydroxybenzyl)-alpha-hydrazinopropionic acid (MK-486) (50 mg/kg i.p. or i.v.), the peripheral formation of 5-hydroxytryptamine-14C (5-HT-14C) from the precursor DL-5-hydroxytryptophan-14C (5-HTP-14C) was reduced by 82 to 100% over a 4-hour interval. There was a marked increase in the penetration of 5-HTP-14C into brain and in the appearance in brain of 5-HT-14C and 5-hydroxyindoleacetic acid-14C (5-HIAA-14C). The cerebral metabolism of 5-HTP-14C in vehicle-pretreated animals appeared to occur mainly in the brain vascular compartment. Following MK-486 pretreatment, the in vivo metabolism of 14C-5-hydroxyindoles in the extracerebral compartment obeys linear kinetics as does the uptake and decarboxylation of 5-HTP-14C in brains. The cerebral turnover of 5-HT-14C appeared to increase as a function of the dose of 5-HTP injected. This phenomenon may be a result of either saturation of brain 5-HT storage sites or nonspecific metabolism of 5-HTP in brain catecholamine neurons. A highly signigificant correlation was found between the brain and blood 5-HIAA-14C levels following MK-486. The adrenal formation of 5-HT, which was not inhibited by MK-486, did not appear to contribute significantly to the blood 5-HIAA-14c levels. These results suggest the possibility that following peripheral decarboxylase inhibition a larger fraction of peripherally measured 5-HIAA may derive from central nervous system 5-HT metabolism.", "contents": "Brain and peripheral metabolism of 5-hydroxytryptophan-14C following peripheral decarboxylase inhibition. Following selective peripheral decarboxylase inhibition, a greater fraction of peripherally measured monoamine metabolites should derive from central nervous system monoamine metabolism. In rats pretreated with the peripheral decarboxylase inhibitor L-alpha-(3,4-dihydroxybenzyl)-alpha-hydrazinopropionic acid (MK-486) (50 mg/kg i.p. or i.v.), the peripheral formation of 5-hydroxytryptamine-14C (5-HT-14C) from the precursor DL-5-hydroxytryptophan-14C (5-HTP-14C) was reduced by 82 to 100% over a 4-hour interval. There was a marked increase in the penetration of 5-HTP-14C into brain and in the appearance in brain of 5-HT-14C and 5-hydroxyindoleacetic acid-14C (5-HIAA-14C). The cerebral metabolism of 5-HTP-14C in vehicle-pretreated animals appeared to occur mainly in the brain vascular compartment. Following MK-486 pretreatment, the in vivo metabolism of 14C-5-hydroxyindoles in the extracerebral compartment obeys linear kinetics as does the uptake and decarboxylation of 5-HTP-14C in brains. The cerebral turnover of 5-HT-14C appeared to increase as a function of the dose of 5-HTP injected. This phenomenon may be a result of either saturation of brain 5-HT storage sites or nonspecific metabolism of 5-HTP in brain catecholamine neurons. A highly signigificant correlation was found between the brain and blood 5-HIAA-14C levels following MK-486. The adrenal formation of 5-HT, which was not inhibited by MK-486, did not appear to contribute significantly to the blood 5-HIAA-14c levels. These results suggest the possibility that following peripheral decarboxylase inhibition a larger fraction of peripherally measured 5-HIAA may derive from central nervous system 5-HT metabolism."}
{"id": "PMID:1084421", "title": "Temperature-induced interconversion of alpha-and beta-adrenoceptors in the frog heart.", "content": "1. The effect of ambient temperature on the properties of adrenoceptors mediating inotropic responses was assessed in isolated frog hearts on the basis of the effects and tissue uptake of alpha- and beta-adrenoceptor antagonists. 2. At temperatures of 23degree C and above inotropic responses to adrenaline were antagonized by propranolol (0-4-4-0muM), but were unaffected by phentolamine (26-5muM) and were potentiated by phenoxybenzamine (POB) (0-7-29-5muM). Below 17degree C the activity of propranolol was reduced at least tenfold, and the alpha-adrenoceptor antagonists inhibited responses to both adrenaline and isoprenaline, but not those to CaCL2. 3. The responses of hearts exposed to POB at 14degree C and then tested, after thorough washing, at both 14 and 24degree C were similarly inhibited at both temperatures, i.e. the usual beta-adrenoceptor response did not appear at the higher temperature. Conversely, exposure to POB at 24degree C produced only potentiation at both test temperatures. 4. Parallel to the reciprocal changes in their blocking actions, significantly more (14C)propranolol was retained by hearts exposed at high temperatures and significantly more (3H)POB was bound to the myocardium at low temperatures. Changes in binding and in the pharmaco logical effects of both blocking agents occurred entirely within a relatively narrow temperature range (17-22degree C) Parallel to the change from alpha- to beta-adrenoceptor characteristics with increasing temperature, the sensitivity of the hearts to adrenaline increased about tenfold. 5. Phentolamine (26-5muM) effectively protected hearts from block by (3H)POB at 14degree C, unmasked a potentiation of responses to adrenaline equivalent to that produced by POB at 24degree C, and reduced binding of the label to approximately the level found in unprotected hearts exposed at the higher temperature. At 24degree C, phentolamine did not alter the potentiation produced by (3H)POB, and reduced binding only slightly. There was no significant temperature differential in the amount of (3H)POB bound in the presence of phentolamine. 6. The results presented indicate a close functional and, probably, morphological association of alpha- and beta-adrenoceptors in the frog heart. It is suggested that the two classes of adrenoceptors may represent allosterie conformations of the same structure.", "contents": "Temperature-induced interconversion of alpha-and beta-adrenoceptors in the frog heart. 1. The effect of ambient temperature on the properties of adrenoceptors mediating inotropic responses was assessed in isolated frog hearts on the basis of the effects and tissue uptake of alpha- and beta-adrenoceptor antagonists. 2. At temperatures of 23degree C and above inotropic responses to adrenaline were antagonized by propranolol (0-4-4-0muM), but were unaffected by phentolamine (26-5muM) and were potentiated by phenoxybenzamine (POB) (0-7-29-5muM). Below 17degree C the activity of propranolol was reduced at least tenfold, and the alpha-adrenoceptor antagonists inhibited responses to both adrenaline and isoprenaline, but not those to CaCL2. 3. The responses of hearts exposed to POB at 14degree C and then tested, after thorough washing, at both 14 and 24degree C were similarly inhibited at both temperatures, i.e. the usual beta-adrenoceptor response did not appear at the higher temperature. Conversely, exposure to POB at 24degree C produced only potentiation at both test temperatures. 4. Parallel to the reciprocal changes in their blocking actions, significantly more (14C)propranolol was retained by hearts exposed at high temperatures and significantly more (3H)POB was bound to the myocardium at low temperatures. Changes in binding and in the pharmaco logical effects of both blocking agents occurred entirely within a relatively narrow temperature range (17-22degree C) Parallel to the change from alpha- to beta-adrenoceptor characteristics with increasing temperature, the sensitivity of the hearts to adrenaline increased about tenfold. 5. Phentolamine (26-5muM) effectively protected hearts from block by (3H)POB at 14degree C, unmasked a potentiation of responses to adrenaline equivalent to that produced by POB at 24degree C, and reduced binding of the label to approximately the level found in unprotected hearts exposed at the higher temperature. At 24degree C, phentolamine did not alter the potentiation produced by (3H)POB, and reduced binding only slightly. There was no significant temperature differential in the amount of (3H)POB bound in the presence of phentolamine. 6. The results presented indicate a close functional and, probably, morphological association of alpha- and beta-adrenoceptors in the frog heart. It is suggested that the two classes of adrenoceptors may represent allosterie conformations of the same structure."}
{"id": "PMID:1084423", "title": "X-linked recessive type of pure spastic paraplegia in a large pedigree: absence of detectable linkage with Xg.", "content": "A family with 24 males affected by an X-linked type of spastic paraplegia is reported. Twelve affected members were personally examined showing the pure form of the disease. Half of the affected males had many descendants, all normal. Linkage studies strongly suggest that this X-linked form of spastic paraplegia and Xg loci are not at a measurable distance on the X chromosome.", "contents": "X-linked recessive type of pure spastic paraplegia in a large pedigree: absence of detectable linkage with Xg. A family with 24 males affected by an X-linked type of spastic paraplegia is reported. Twelve affected members were personally examined showing the pure form of the disease. Half of the affected males had many descendants, all normal. Linkage studies strongly suggest that this X-linked form of spastic paraplegia and Xg loci are not at a measurable distance on the X chromosome."}
{"id": "PMID:1084424", "title": "Tryptophan metabolism and the occurrence of amino acids and serotonin in the leech (Hirudo medicinalis) nervous system.", "content": "The occurrence and distribution of serotonin (5-HT) and amino acids along the central nerve chain of the leech, Hirudo medicinalis, was analyzed using dansyl-chloride in order to obtain information on the functional roles of amines and amino acids. There was no statistical variation in the occurrence of most of the amino acids or serotonin in the different parts of the dorsal nerve chain of the leech. A variation in the serotonin content was found during the year with the highest level occurring in winter. Leech ganglia also accumulated 3H-tryptophan and metabolized part of it to form 5-HTP, 5-HT, and 5-HIAA. The results are discussed from the point of view of 5-HT being a transmitter substance.", "contents": "Tryptophan metabolism and the occurrence of amino acids and serotonin in the leech (Hirudo medicinalis) nervous system. The occurrence and distribution of serotonin (5-HT) and amino acids along the central nerve chain of the leech, Hirudo medicinalis, was analyzed using dansyl-chloride in order to obtain information on the functional roles of amines and amino acids. There was no statistical variation in the occurrence of most of the amino acids or serotonin in the different parts of the dorsal nerve chain of the leech. A variation in the serotonin content was found during the year with the highest level occurring in winter. Leech ganglia also accumulated 3H-tryptophan and metabolized part of it to form 5-HTP, 5-HT, and 5-HIAA. The results are discussed from the point of view of 5-HT being a transmitter substance."}
{"id": "PMID:1084425", "title": "Changes in occupation after aortocoronary vein-bypass operation.", "content": "The effect of aortocoronary vein-bypass operation on changes in occupational status was investigated in a cohort of 893 men. The median period of time between the operation and follow-up evaluation of postoperative occupational status was 14 months. The patients were divided into two age groups, with 55 years as the dividing point. The results showed that 11% of the younger men and 26% of the older group who were employed before the operation retired following the operation. In addition, 30% of the patients changed their occupational status following the operation. When occupational changes also resulted in changes in physical demands, 49% of the patients took new jobs that were less demanding physically, while 40% took jobs that were more demanding. Close to 9% of the patients were retired prior to the operation; about one fifth of them came out of retirement following the operation.", "contents": "Changes in occupation after aortocoronary vein-bypass operation. The effect of aortocoronary vein-bypass operation on changes in occupational status was investigated in a cohort of 893 men. The median period of time between the operation and follow-up evaluation of postoperative occupational status was 14 months. The patients were divided into two age groups, with 55 years as the dividing point. The results showed that 11% of the younger men and 26% of the older group who were employed before the operation retired following the operation. In addition, 30% of the patients changed their occupational status following the operation. When occupational changes also resulted in changes in physical demands, 49% of the patients took new jobs that were less demanding physically, while 40% took jobs that were more demanding. Close to 9% of the patients were retired prior to the operation; about one fifth of them came out of retirement following the operation."}
{"id": "PMID:1084426", "title": "Cerebral ventricular enlargement. Chronic alcoholics examined by computerized tomography.", "content": "Computerized tomography was used to measure cerebral ventricular size in hospitalized alcoholic patients, all of whom had evidence of liver disease. Twelve alcoholic patients with neurologic symptoms such as withdrawal seizures, neuropathy, and drug overdose were included. All these patients had normal results from the mental status examinations by the time of discharge. Alcoholic patients had a much higher mean ventricular size compared to 60 control patients. One third of the alcoholics had markedly enlarged ventricles as opposed to only one of the 60 controls.", "contents": "Cerebral ventricular enlargement. Chronic alcoholics examined by computerized tomography. Computerized tomography was used to measure cerebral ventricular size in hospitalized alcoholic patients, all of whom had evidence of liver disease. Twelve alcoholic patients with neurologic symptoms such as withdrawal seizures, neuropathy, and drug overdose were included. All these patients had normal results from the mental status examinations by the time of discharge. Alcoholic patients had a much higher mean ventricular size compared to 60 control patients. One third of the alcoholics had markedly enlarged ventricles as opposed to only one of the 60 controls."}
{"id": "PMID:1084428", "title": "Retrograde aortic dissection occurring during cardiopulmonary bypass. Successful repair and concominant subclavian-to-coronary artery vein bypass.", "content": "A retrograde aortic dissection that occurred during cardiopulmonary bypass was successfully repaired. The cause of dissection was related to the jet of retrograde perfusion. The use of subclavian-to-coronary artery vein bypass when the ascending aorta is not suitable for vein anastomosis because of dissection or aneurysms appears to be a satisfactory alternative to aorta-coronary bypass.", "contents": "Retrograde aortic dissection occurring during cardiopulmonary bypass. Successful repair and concominant subclavian-to-coronary artery vein bypass. A retrograde aortic dissection that occurred during cardiopulmonary bypass was successfully repaired. The cause of dissection was related to the jet of retrograde perfusion. The use of subclavian-to-coronary artery vein bypass when the ascending aorta is not suitable for vein anastomosis because of dissection or aneurysms appears to be a satisfactory alternative to aorta-coronary bypass."}
{"id": "PMID:1084432", "title": "Effects of L-glutamine on acetylsalicylic acid-induced gastric lesions in normal and cirrhotic rats.", "content": "Cirrhosis of the liver in rats was induced by the administration of carbon tetrachloride (0.1 ml/100 g of body weight, s.c.) biweekly for 13 weeks. In the pylorus ligation preparation, acetylsalicylic acid (ASA) 100 mg/kg p.o. induced much more serious gastric damage in CC14-induced cirrhotic rats as compared with rats with a normal liver. L-glutamine 750 mg/kg p.o. prevented the ASA-induced gastric lesions in both normal and cirrhotic rats, even though the degree of the inhibition was weaker in cirrhotic rats. Gastric analysis indicated that L-glutamine 750 mg/kg p.o. markedly inhibited the gastric ionic changes (acid back diffusion) in response to ASA in both cirrhotic and normal rats.", "contents": "Effects of L-glutamine on acetylsalicylic acid-induced gastric lesions in normal and cirrhotic rats. Cirrhosis of the liver in rats was induced by the administration of carbon tetrachloride (0.1 ml/100 g of body weight, s.c.) biweekly for 13 weeks. In the pylorus ligation preparation, acetylsalicylic acid (ASA) 100 mg/kg p.o. induced much more serious gastric damage in CC14-induced cirrhotic rats as compared with rats with a normal liver. L-glutamine 750 mg/kg p.o. prevented the ASA-induced gastric lesions in both normal and cirrhotic rats, even though the degree of the inhibition was weaker in cirrhotic rats. Gastric analysis indicated that L-glutamine 750 mg/kg p.o. markedly inhibited the gastric ionic changes (acid back diffusion) in response to ASA in both cirrhotic and normal rats."}
{"id": "PMID:1084444", "title": "Estimation of cell surface associated protease activity and its application to lymphocytes.", "content": "A new method has been developed to estimate proteolytic activity available at the cell surface. Radioiodinated protein substrates are covalently linked to modified polystyrene-divinylbenzene beads with various diameters. These beads are presented to viable cells. Secreted enzyme activity is estimated when no contact occurs between beads and cells. Surface associated proteolytic activity is estimated by the increased rate of iodinated peptide release due to a contact between beads and cells. This method was applied to various lymphocyte preparations. In the absence of serum, mouse spleen lymphocytes produce three- to fourfold higher proteolytic activity than lymph node cells. This activity is completely inhibited by serum diluted 1:10. Since the proteolysis is so marked in the case of spleen cells, one must conclude that lymphocytes removed from the serum and treated in buffered mediums at 37 degrees C have enzymatically altered surface properties. Cell surface associated enzyme activity was measured using rat lymph node lymphocytes with less than 0.1% contamination by granulocytes. This predominantly thymus derived, T cell population had 30% increase in proteolysis due to contact between cells and solid-phase localized substrate of casein. The released enzymatic activity was inhibited by diisopropylfluorophosphate, but its effect on the surface associated enzyme activity remains questionable since it perturbs several membrane functions.", "contents": "Estimation of cell surface associated protease activity and its application to lymphocytes. A new method has been developed to estimate proteolytic activity available at the cell surface. Radioiodinated protein substrates are covalently linked to modified polystyrene-divinylbenzene beads with various diameters. These beads are presented to viable cells. Secreted enzyme activity is estimated when no contact occurs between beads and cells. Surface associated proteolytic activity is estimated by the increased rate of iodinated peptide release due to a contact between beads and cells. This method was applied to various lymphocyte preparations. In the absence of serum, mouse spleen lymphocytes produce three- to fourfold higher proteolytic activity than lymph node cells. This activity is completely inhibited by serum diluted 1:10. Since the proteolysis is so marked in the case of spleen cells, one must conclude that lymphocytes removed from the serum and treated in buffered mediums at 37 degrees C have enzymatically altered surface properties. Cell surface associated enzyme activity was measured using rat lymph node lymphocytes with less than 0.1% contamination by granulocytes. This predominantly thymus derived, T cell population had 30% increase in proteolysis due to contact between cells and solid-phase localized substrate of casein. The released enzymatic activity was inhibited by diisopropylfluorophosphate, but its effect on the surface associated enzyme activity remains questionable since it perturbs several membrane functions."}
{"id": "PMID:1084445", "title": "Light-induced tetracycline accumulation by Rhodopseudomonas sphaeroides.", "content": "Light has been used as a primary energy source in studies of tetracycline transport by Rhodopseudomonas sphaeroides. Accumulation of the antibiotic occurs in light, while efflux occurs in dark. Both fluorescence enhancement and radioisotopic tracing have been used to monitor transport. Km's obtained from both techniques are similar. Light-induced accumulation of tetracyclines is inhibited by a variety of inhibitors, including antimycin A, N-ethylmaleimide, carbonylcyanide m-chlorophenylhydrazone, and 2,4-dinitrophenol. A rapid efflux is observed after loading when cells are placed in the dark or treated with inhibitors.", "contents": "Light-induced tetracycline accumulation by Rhodopseudomonas sphaeroides. Light has been used as a primary energy source in studies of tetracycline transport by Rhodopseudomonas sphaeroides. Accumulation of the antibiotic occurs in light, while efflux occurs in dark. Both fluorescence enhancement and radioisotopic tracing have been used to monitor transport. Km's obtained from both techniques are similar. Light-induced accumulation of tetracyclines is inhibited by a variety of inhibitors, including antimycin A, N-ethylmaleimide, carbonylcyanide m-chlorophenylhydrazone, and 2,4-dinitrophenol. A rapid efflux is observed after loading when cells are placed in the dark or treated with inhibitors."}
{"id": "PMID:1084446", "title": "Support of the myocardium with intra-aortic balloon counterpulsation following cardiopulmonary bypass.", "content": "Intra-aortic balloon counterpulsation (IABC) was instituted in 27 patients in cardiogenic shock who had undergone cardiopulmonary bypass (CPB). Patients who required IABC either prior to CPB or else to be weaned from CPB had a survival rate of 22 per cent (2/9). In contrast, the survival rate for those patients requiring IABC after CPB had been discontinued was 72 per cent (13/18). The composition of these two groups was different. The first group contained primarily patients with either severe coronary artery disease or aortic valve stenosis, whereas the latter group contained patients with either less severe coronary artery disease or mitral valve disease. Most patients had an initial satisfactory response to IABC. The over-all survival rate was 55 per cent (15/27). IABC was particularly beneficial for those patients in cardiogenic shock following CPB who had not had massive and irreveraible myocardial injury.", "contents": "Support of the myocardium with intra-aortic balloon counterpulsation following cardiopulmonary bypass. Intra-aortic balloon counterpulsation (IABC) was instituted in 27 patients in cardiogenic shock who had undergone cardiopulmonary bypass (CPB). Patients who required IABC either prior to CPB or else to be weaned from CPB had a survival rate of 22 per cent (2/9). In contrast, the survival rate for those patients requiring IABC after CPB had been discontinued was 72 per cent (13/18). The composition of these two groups was different. The first group contained primarily patients with either severe coronary artery disease or aortic valve stenosis, whereas the latter group contained patients with either less severe coronary artery disease or mitral valve disease. Most patients had an initial satisfactory response to IABC. The over-all survival rate was 55 per cent (15/27). IABC was particularly beneficial for those patients in cardiogenic shock following CPB who had not had massive and irreveraible myocardial injury."}
{"id": "PMID:1084447", "title": "Aneurysm of the sinus of Valsalva producing obstruction of the left main coronary artery.", "content": "An aneurysm of the left sinus of Valsalva compressed the left main coronary artery and the patient experienced anginal pain. Surgical correction consisted of obliteration of the orifice of the aneurysm, aortic valve replacement, and a saphenous vein bypass from the ascending aorta to the distal left anterior descending coronary artery. Postoperative studies revealed excellent function of the prosthetic valve, no recurrence of the aneurysm and retrograde filling of the left anterior descending and circumflex coronary arteries. Three years after the operation, the patient is asymptomatic.", "contents": "Aneurysm of the sinus of Valsalva producing obstruction of the left main coronary artery. An aneurysm of the left sinus of Valsalva compressed the left main coronary artery and the patient experienced anginal pain. Surgical correction consisted of obliteration of the orifice of the aneurysm, aortic valve replacement, and a saphenous vein bypass from the ascending aorta to the distal left anterior descending coronary artery. Postoperative studies revealed excellent function of the prosthetic valve, no recurrence of the aneurysm and retrograde filling of the left anterior descending and circumflex coronary arteries. Three years after the operation, the patient is asymptomatic."}
{"id": "PMID:1084448", "title": "Aspirin and prophylaxis of thromboembolic complications in patients with substitute heart valves.", "content": "Anticoagulation therapy with acenocoumarin or with anticoagulants plus aspirin was given to 65 and 57 patients, respectively, with cardiac valve replacement. The follow-up was 1,462 months (22.5 months per patient) for the first group and 1,411 months (24.7 months per patient) for the second group. The frequency of embolic accidents was significantly lower in the group taking aspirin: Thirteen thromboembolic accidents were detected in patients receiving the anticoagulant and 3 in the group receiving the anticoagulant plus aspirin. These figures represent a 20.3 per cent incidence (one each 9.3 years of treatment) for the anticoagulant group and a 5.2 per cent incidence (one accident each 39.1 years of treatment) for the other group. The statistical significance between groups is p less than 0.005. There was no difference in the hemorrhagic risk between the two groups. We conclude that the use of an anticoagulant plus aspirin is a good and safe therapy for the prevention of thromboembolism in these patients.", "contents": "Aspirin and prophylaxis of thromboembolic complications in patients with substitute heart valves. Anticoagulation therapy with acenocoumarin or with anticoagulants plus aspirin was given to 65 and 57 patients, respectively, with cardiac valve replacement. The follow-up was 1,462 months (22.5 months per patient) for the first group and 1,411 months (24.7 months per patient) for the second group. The frequency of embolic accidents was significantly lower in the group taking aspirin: Thirteen thromboembolic accidents were detected in patients receiving the anticoagulant and 3 in the group receiving the anticoagulant plus aspirin. These figures represent a 20.3 per cent incidence (one each 9.3 years of treatment) for the anticoagulant group and a 5.2 per cent incidence (one accident each 39.1 years of treatment) for the other group. The statistical significance between groups is p less than 0.005. There was no difference in the hemorrhagic risk between the two groups. We conclude that the use of an anticoagulant plus aspirin is a good and safe therapy for the prevention of thromboembolism in these patients."}
{"id": "PMID:1084449", "title": "The effect of hemodilution on plasma levels of cortisol and free cortisol.", "content": "The effect of hemodilution on plasma levels of total cortisol and free cortisol was studied in 12 patients undergoing open-heart surgery. In each case, at the onset of extracorporeal circulation (ECC) the hematocrit value was rapidly reduced to 25 to 30 per cent and was maintained around this level during perfusion. Total plasma cortisol levels fell significantly with the hemodilution (p less than 0.0001), but the plasma concentration of free, biologically active cortisol was maintained at its pre-dilution level because of an increase in the percentage of plasma cortisol existing in the free form. There was a highly significant correlation between the degree of hemodilution and the percentage rise in the free cortisol fraction (r = 0.79; p less than 0.001). These results indicate the presence of a compensatory mechanism associated with cortisol binding which maintains the plasma level of free, biologically active cortisol when hemodilution has reduced the plasma level of total cortisol.", "contents": "The effect of hemodilution on plasma levels of cortisol and free cortisol. The effect of hemodilution on plasma levels of total cortisol and free cortisol was studied in 12 patients undergoing open-heart surgery. In each case, at the onset of extracorporeal circulation (ECC) the hematocrit value was rapidly reduced to 25 to 30 per cent and was maintained around this level during perfusion. Total plasma cortisol levels fell significantly with the hemodilution (p less than 0.0001), but the plasma concentration of free, biologically active cortisol was maintained at its pre-dilution level because of an increase in the percentage of plasma cortisol existing in the free form. There was a highly significant correlation between the degree of hemodilution and the percentage rise in the free cortisol fraction (r = 0.79; p less than 0.001). These results indicate the presence of a compensatory mechanism associated with cortisol binding which maintains the plasma level of free, biologically active cortisol when hemodilution has reduced the plasma level of total cortisol."}
{"id": "PMID:1084450", "title": "Left ventricular function and coronary obstruction as predictors of survival following aorta-coronary bypass.", "content": "A retrospective analysis was undertaken of clinical data and catheterization studies of 151 consecutive unselected patients who underwent aorta-coronary bypass at the University of Kansas Medical Center between 1971 and 1973. The purpose was to determine the effect of preoperative left ventricular function and extent and severity of coronary artery obstruction on operative mortality rate and long-term survival. The postoperative follow-up period ranged from 10 to 49 months and averaged 26 months. Left ventricular function was assessed by qualitative analysis of left ventricular angiograms. Severity of coronary obstruction was quantified by scoring coronary arteriograms according to the system of Friesinger and associates. Patients with normal or near normal ventriculograms were considered to have good left ventricular function. Patients showing moderate or severe impairment of contraction were considered to have poor left ventricular function. Obstruction scores ranging from 2 to 7 points were classified as low scores, and scores from 8 to 15 points were classified as high scores. Four groups of patients were identified based upon preoperative left ventricular function and obstruction severity: Group I, 29 patients with good left ventricular function and low scores; Group II, 22 patients with poor left ventricular function and low scores. Group III, 28 patients with good left ventricular function and high scores. Elective aorta-coronary bypass in these three groups was accompanied by no operative or late deaths. Group IV comprised 72 patients with poor left ventricular function and high scores. In this group there was a 10 per cent operative mortality rate (7 of 72 patients) and a 5 per cent year late mortality rate. Relief of angina occurred equally in all groups. Thus operative risk can be prospectively determined by analysis of left ventricular function and severity of coronary obstruction. Surgical treatment resulted in negligible operative and late mortality rates (0 per cent) in all patients except those in whom poor ventricular function was accompanied by severe and diffuse coronary artery obstruction. Operation should be offered to this latter group (Group IV) despite the higher operative and postoperative risk because of salutary postoperative results.", "contents": "Left ventricular function and coronary obstruction as predictors of survival following aorta-coronary bypass. A retrospective analysis was undertaken of clinical data and catheterization studies of 151 consecutive unselected patients who underwent aorta-coronary bypass at the University of Kansas Medical Center between 1971 and 1973. The purpose was to determine the effect of preoperative left ventricular function and extent and severity of coronary artery obstruction on operative mortality rate and long-term survival. The postoperative follow-up period ranged from 10 to 49 months and averaged 26 months. Left ventricular function was assessed by qualitative analysis of left ventricular angiograms. Severity of coronary obstruction was quantified by scoring coronary arteriograms according to the system of Friesinger and associates. Patients with normal or near normal ventriculograms were considered to have good left ventricular function. Patients showing moderate or severe impairment of contraction were considered to have poor left ventricular function. Obstruction scores ranging from 2 to 7 points were classified as low scores, and scores from 8 to 15 points were classified as high scores. Four groups of patients were identified based upon preoperative left ventricular function and obstruction severity: Group I, 29 patients with good left ventricular function and low scores; Group II, 22 patients with poor left ventricular function and low scores. Group III, 28 patients with good left ventricular function and high scores. Elective aorta-coronary bypass in these three groups was accompanied by no operative or late deaths. Group IV comprised 72 patients with poor left ventricular function and high scores. In this group there was a 10 per cent operative mortality rate (7 of 72 patients) and a 5 per cent year late mortality rate. Relief of angina occurred equally in all groups. Thus operative risk can be prospectively determined by analysis of left ventricular function and severity of coronary obstruction. Surgical treatment resulted in negligible operative and late mortality rates (0 per cent) in all patients except those in whom poor ventricular function was accompanied by severe and diffuse coronary artery obstruction. Operation should be offered to this latter group (Group IV) despite the higher operative and postoperative risk because of salutary postoperative results."}
{"id": "PMID:1084451", "title": "On a model for ontogenic development.", "content": "A theory to explain both the endogenous control of differentiation and programmed organismal aging as the result of one fundamental regulatory mechanism is advanced. It is postulated that these phenomena are linked to cell division, which is presumed to be the primary unit of measurement for the organism's maturation and aging. The hypothesis developed here holds that a genetic locus (DSI), modulating both cell division and enzyme induction, is first activated and then inactivated with each cycle. The number of DSIs in the genome of each cell is finite, cellular sensescence occurring when the genome is depleted of functional DSIs. Protein inducers are linked to specific DSIs, which are only transcribed at appropriate times during ontogeny, this in turn being a function of the number of DSIs previously inactivated during earlier mitoses. Experimental approaches which could test this model are discussed.", "contents": "On a model for ontogenic development. A theory to explain both the endogenous control of differentiation and programmed organismal aging as the result of one fundamental regulatory mechanism is advanced. It is postulated that these phenomena are linked to cell division, which is presumed to be the primary unit of measurement for the organism's maturation and aging. The hypothesis developed here holds that a genetic locus (DSI), modulating both cell division and enzyme induction, is first activated and then inactivated with each cycle. The number of DSIs in the genome of each cell is finite, cellular sensescence occurring when the genome is depleted of functional DSIs. Protein inducers are linked to specific DSIs, which are only transcribed at appropriate times during ontogeny, this in turn being a function of the number of DSIs previously inactivated during earlier mitoses. Experimental approaches which could test this model are discussed."}
{"id": "PMID:1084456", "title": "[Nasopharyngeal culture results in children with acute otitis media].", "content": "Nasopharyngeal cultures in 463 children, suffering from otitis media were examined in 1968-1970. In 45% of cases H. influenzae, D. pneumoniae, S. aureus and beta hemolytic streptococci were found. These are thought to be the causative organisms for acute otitis media, however their dominance may vary. In our findings H. influenzae came first. 102 of 463 children, that is, 21.9% showed this organism. D. pneumoniae was second with 10.7%, which is generally considered as the primary cause of bacterial otitis media in children.", "contents": "[Nasopharyngeal culture results in children with acute otitis media]. Nasopharyngeal cultures in 463 children, suffering from otitis media were examined in 1968-1970. In 45% of cases H. influenzae, D. pneumoniae, S. aureus and beta hemolytic streptococci were found. These are thought to be the causative organisms for acute otitis media, however their dominance may vary. In our findings H. influenzae came first. 102 of 463 children, that is, 21.9% showed this organism. D. pneumoniae was second with 10.7%, which is generally considered as the primary cause of bacterial otitis media in children."}
{"id": "PMID:1084452", "title": "Age-related changes in ConA- and LPS-induced lymphocyte transformation. I. Effect of culture conditions on mitogen responses of blood and spleen lymphocytes from young and aged rats.", "content": "Age-related changes in LPS- and ConA-responsiveness of rat spleen lymphocytes as judged by 14C-TdR incorporation were studied. It was found that responses to both mitogens decreased with advancing age. This report shows that the reduced 14C-TdR incorporation could not be attributed to decreased cell survival or viability of spleen lymphocytes from old rats, to delayed proliferation of the old lymphocytes, or to differences in minimum mitogen doses required for optimal stimulation. The results suggest that the observed decrease is due to a decrease in the number of mitogen responsive cells. The response to LPS was even more depressed than was the response to ConA. The response to ConA in whole blood is also shown to decline with ageing at multiple mitogen doses.", "contents": "Age-related changes in ConA- and LPS-induced lymphocyte transformation. I. Effect of culture conditions on mitogen responses of blood and spleen lymphocytes from young and aged rats. Age-related changes in LPS- and ConA-responsiveness of rat spleen lymphocytes as judged by 14C-TdR incorporation were studied. It was found that responses to both mitogens decreased with advancing age. This report shows that the reduced 14C-TdR incorporation could not be attributed to decreased cell survival or viability of spleen lymphocytes from old rats, to delayed proliferation of the old lymphocytes, or to differences in minimum mitogen doses required for optimal stimulation. The results suggest that the observed decrease is due to a decrease in the number of mitogen responsive cells. The response to LPS was even more depressed than was the response to ConA. The response to ConA in whole blood is also shown to decline with ageing at multiple mitogen doses."}
{"id": "PMID:1084457", "title": "Medical decision profiles derived from a symptom questionnaire.", "content": "The introduction of formalized systems for data acquisition provide a basis for the investigation of medical decision making. Patients admitted to a general medical ward completed a comprehensive, medical history. Decisions relating to their subsequent diagnosis, investigation and management were made after complete investigation and were recorded with their questionnaire responses. A computer programme was developed to compare questionnaire responses from patients who did, and patients who did not have each particular decision made, and to assign a value to each question depending on the significance of its association with the decision. The set of values, one for each question, was termed a decision profile and was characteristic of that decision.", "contents": "Medical decision profiles derived from a symptom questionnaire. The introduction of formalized systems for data acquisition provide a basis for the investigation of medical decision making. Patients admitted to a general medical ward completed a comprehensive, medical history. Decisions relating to their subsequent diagnosis, investigation and management were made after complete investigation and were recorded with their questionnaire responses. A computer programme was developed to compare questionnaire responses from patients who did, and patients who did not have each particular decision made, and to assign a value to each question depending on the significance of its association with the decision. The set of values, one for each question, was termed a decision profile and was characteristic of that decision."}
{"id": "PMID:1084460", "title": "Detection of cytotoxic lymphoid spleen cells from STU-mice with Moloney sarcoma by a 3H-proline microcytotoxicity assay.", "content": "A microcytoxicity assay with 3H-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from STU inbred mice inoculated with Moloney sarcoma virus (MSV-M) or ascitic MSV-M tumor cells. The target cell line was derived from ascitic MSV-M tumor cells. With regard to the specificity of the assay nonimmune slpeen cells displayed no or only a weak cytotoxicity against these cells, and this was also the case when 3H-proline-labeled secondary cultures of syngeneic mouse embryo cells were exposed to both sensitized and nonimmune spleen cells. The time-course pattern of the development of cytotoxic lymphoid spleen cells in STU mice inoculated intramuscularly either with MSV-M or ascitic MSV-M tumor cells was studied. At the stages of tumor development, peak tumor size, and tumor regression the lymphoid spleen cell preparations were found to have relatively strong cytotoxic activity independent of whether the tumor was induced by MSV-M inoculation or tumor cell transplantation. However, in the latter case effector cells appeared earlier and were demonstrable for a longer period than in MSV-M-inoculated mice. Anti-theta serum treatment of lymphoid spleen cells taken at the stage of peak tumor size abrogated the cytotoxic activity or diminished it considerably indicating a T-lymphocyte response.", "contents": "Detection of cytotoxic lymphoid spleen cells from STU-mice with Moloney sarcoma by a 3H-proline microcytotoxicity assay. A microcytoxicity assay with 3H-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from STU inbred mice inoculated with Moloney sarcoma virus (MSV-M) or ascitic MSV-M tumor cells. The target cell line was derived from ascitic MSV-M tumor cells. With regard to the specificity of the assay nonimmune slpeen cells displayed no or only a weak cytotoxicity against these cells, and this was also the case when 3H-proline-labeled secondary cultures of syngeneic mouse embryo cells were exposed to both sensitized and nonimmune spleen cells. The time-course pattern of the development of cytotoxic lymphoid spleen cells in STU mice inoculated intramuscularly either with MSV-M or ascitic MSV-M tumor cells was studied. At the stages of tumor development, peak tumor size, and tumor regression the lymphoid spleen cell preparations were found to have relatively strong cytotoxic activity independent of whether the tumor was induced by MSV-M inoculation or tumor cell transplantation. However, in the latter case effector cells appeared earlier and were demonstrable for a longer period than in MSV-M-inoculated mice. Anti-theta serum treatment of lymphoid spleen cells taken at the stage of peak tumor size abrogated the cytotoxic activity or diminished it considerably indicating a T-lymphocyte response."}
{"id": "PMID:1084461", "title": "The effect of a lymphotoxic factor from patients with multiple sclerosis on acute lymphosarcoma cell leukemia and acute lymphoblastic leukemia.", "content": "Eight patients with acute lymphosarcoma cell leukemia or acute lymphoblastic leukemia received infusions of plasma containing a lymphotoxic factor. The lymphotoxic factor induced a transient fall in the number of \"blast\" cell forms in the peripheral blood in six of the eight patients but did not produce sustained remissions. Previous investigations have demonstrated that this lymphotoxic factor, found in the plasma of patients with active multiple sclerosis, is of low molecular weight and does not have the properties normally associated with an antibody. This factor appears to selectively interfere with ribonucleic acid synthesis in the thymus-derived lymphocyte. Lymphotoxic factor activity was demonstrated in the serum of one patient during a remission in leukemia induced by a viral illness.", "contents": "The effect of a lymphotoxic factor from patients with multiple sclerosis on acute lymphosarcoma cell leukemia and acute lymphoblastic leukemia. Eight patients with acute lymphosarcoma cell leukemia or acute lymphoblastic leukemia received infusions of plasma containing a lymphotoxic factor. The lymphotoxic factor induced a transient fall in the number of \"blast\" cell forms in the peripheral blood in six of the eight patients but did not produce sustained remissions. Previous investigations have demonstrated that this lymphotoxic factor, found in the plasma of patients with active multiple sclerosis, is of low molecular weight and does not have the properties normally associated with an antibody. This factor appears to selectively interfere with ribonucleic acid synthesis in the thymus-derived lymphocyte. Lymphotoxic factor activity was demonstrated in the serum of one patient during a remission in leukemia induced by a viral illness."}
{"id": "PMID:1084486", "title": "Complement consumption in fresh blood sera by double culture of Paramecium caudatum and Aerobacter cloacae compared with survival and stage of disease in some malignant tumors in man.", "content": "Using the complement consumption test by double culture of Paramecium caudatum and Aerobacter cloacae (DPA) as antigen we examined blood sera and compared survival rates and stage of disease in 124 patients with carcinoma of cervix uteri, corpus uteri, ovaries and breast. The evidence suggests that bacteria Aerobacter cloacae constitute the proper antigenically effective substances of DPA. The intensity of reaction and therapy the relative titres of antibodies detected by the test were expressed by the amount of complement consumed in the reaction and designated by the abbreviation \"NMHD\", i. e. number of minimum haemolytic doses of complement fixed by antigen in the reaction. Patients with NMHD above 8 (i. e. with an intensive reaction corresponding to a high level of antibodies) exhibited partly higher three-year survival rates -- starting from the time of diagnosis, partly more often the clinical stages of disease I or II as compared with patients with NMHD below 4. The best survival rates were found in patients with NMHD above 8 and clinical stage I or II. NMHD values in patients with the initial period of disease and in healthy women did not differ substantially.", "contents": "Complement consumption in fresh blood sera by double culture of Paramecium caudatum and Aerobacter cloacae compared with survival and stage of disease in some malignant tumors in man. Using the complement consumption test by double culture of Paramecium caudatum and Aerobacter cloacae (DPA) as antigen we examined blood sera and compared survival rates and stage of disease in 124 patients with carcinoma of cervix uteri, corpus uteri, ovaries and breast. The evidence suggests that bacteria Aerobacter cloacae constitute the proper antigenically effective substances of DPA. The intensity of reaction and therapy the relative titres of antibodies detected by the test were expressed by the amount of complement consumed in the reaction and designated by the abbreviation \"NMHD\", i. e. number of minimum haemolytic doses of complement fixed by antigen in the reaction. Patients with NMHD above 8 (i. e. with an intensive reaction corresponding to a high level of antibodies) exhibited partly higher three-year survival rates -- starting from the time of diagnosis, partly more often the clinical stages of disease I or II as compared with patients with NMHD below 4. The best survival rates were found in patients with NMHD above 8 and clinical stage I or II. NMHD values in patients with the initial period of disease and in healthy women did not differ substantially."}
{"id": "PMID:1084490", "title": "Scintigraphy in lymphomas and leukemia of the brain.", "content": "Scintigraphy with 99mTc-pertechnetate made it possible to detect focal brain lesions in 4 patients with primary malignant lymphomas of the brain, one patient with CNS involvement with lymphatic immunoblastoma, one patient with S\u00e9zary syndrome (mycosis fungoides and T cell leukemia) and one child with a space occupying infiltration of myeloblastic leukemia. The scintigraphic results were verified by morphological data from biopsy or autopsy.", "contents": "Scintigraphy in lymphomas and leukemia of the brain. Scintigraphy with 99mTc-pertechnetate made it possible to detect focal brain lesions in 4 patients with primary malignant lymphomas of the brain, one patient with CNS involvement with lymphatic immunoblastoma, one patient with S\u00e9zary syndrome (mycosis fungoides and T cell leukemia) and one child with a space occupying infiltration of myeloblastic leukemia. The scintigraphic results were verified by morphological data from biopsy or autopsy."}
{"id": "PMID:1084487", "title": "[Artery-nerve conflict. Involvement of the acoustic-facial group].", "content": "Six cases of \"Conflit Art\u00e8re-Nerf\" concerning acoustico-facial nerves discovered by surgical exploration of cerebello-pontine angle. This conflit is due to an encircling middle cerebellar artery constricting the nerve or by a large Internal Auditory Artery lying upon it. Dissection of the surroundings arachnoidal tracts had given in these cases good results.", "contents": "[Artery-nerve conflict. Involvement of the acoustic-facial group]. Six cases of \"Conflit Art\u00e8re-Nerf\" concerning acoustico-facial nerves discovered by surgical exploration of cerebello-pontine angle. This conflit is due to an encircling middle cerebellar artery constricting the nerve or by a large Internal Auditory Artery lying upon it. Dissection of the surroundings arachnoidal tracts had given in these cases good results."}
{"id": "PMID:1084491", "title": "Computerized tomography: abnormal intracerebral collections of blood in children.", "content": "Three children with unusual abnormal intracerebral collections of blood were examined by computerized tomography. These cases demonstrate the complementary relationship of this technique with other neuroradiological diagnostic methods. An intracerebral aneurysm and an arteriovenous malformation of the vein of Galen are demonstrated on computerized tomograms.", "contents": "Computerized tomography: abnormal intracerebral collections of blood in children. Three children with unusual abnormal intracerebral collections of blood were examined by computerized tomography. These cases demonstrate the complementary relationship of this technique with other neuroradiological diagnostic methods. An intracerebral aneurysm and an arteriovenous malformation of the vein of Galen are demonstrated on computerized tomograms."}
{"id": "PMID:1084492", "title": "Computerized axial transverse tomography in normal pressure hydrocephalus.", "content": "Twenty patients with normal pressure hydrocephalus were examined by computerized axial transverse tomographic (CT) scanning and the results were compared with pneumoencephalographic findings and clinical responses to ventricular shunting. The CT scan revealed significant cortical atrophy, not demonstrated by pneumoencephalogram in seven patients. The cortical atrophy was confirmed by autopsy in one case. This examination also revealed greatly enlarged ventricles (25 to 40 per cent of intracranial volume) in eight patients up to 4 years after ventricular shunting. There was no relationship between the presence or absence of cortical atrophy, or the postoperative ventricular size, and the clinical responses to ventricular shunting. Current concepts of normal pressure hydrocephalus must be revised in view of these findings.", "contents": "Computerized axial transverse tomography in normal pressure hydrocephalus. Twenty patients with normal pressure hydrocephalus were examined by computerized axial transverse tomographic (CT) scanning and the results were compared with pneumoencephalographic findings and clinical responses to ventricular shunting. The CT scan revealed significant cortical atrophy, not demonstrated by pneumoencephalogram in seven patients. The cortical atrophy was confirmed by autopsy in one case. This examination also revealed greatly enlarged ventricles (25 to 40 per cent of intracranial volume) in eight patients up to 4 years after ventricular shunting. There was no relationship between the presence or absence of cortical atrophy, or the postoperative ventricular size, and the clinical responses to ventricular shunting. Current concepts of normal pressure hydrocephalus must be revised in view of these findings."}
{"id": "PMID:1084493", "title": "Correlation between computerized transaxial tomography and radionuclide cisternography in dementia.", "content": "A study of the findings on cisternograms and computerized transaxial tomography (CTT) of 144 patients showed good correlation between the two tests. Patients with a cisternographic pattern of communicating hydrocephalus tended to show more severe degrees of lateral ventricular dilation, with involvement of the third and fourth ventricles and relative sparing of the cerebral sulci. Patients with a cisternographic pattern of cerebral atrophy showed lesser degrees of lateral ventricular dilatation and more severe degrees of dilatation of the cerebral sulci. The statistical significance of the individual CTT findings in these patients is presented, and a cheme for interpretation is proposed. The study shows that CTT can replace pneumoencephalography in evaluating patients with dementia, while cisternography contributes complementary information.", "contents": "Correlation between computerized transaxial tomography and radionuclide cisternography in dementia. A study of the findings on cisternograms and computerized transaxial tomography (CTT) of 144 patients showed good correlation between the two tests. Patients with a cisternographic pattern of communicating hydrocephalus tended to show more severe degrees of lateral ventricular dilation, with involvement of the third and fourth ventricles and relative sparing of the cerebral sulci. Patients with a cisternographic pattern of cerebral atrophy showed lesser degrees of lateral ventricular dilatation and more severe degrees of dilatation of the cerebral sulci. The statistical significance of the individual CTT findings in these patients is presented, and a cheme for interpretation is proposed. The study shows that CTT can replace pneumoencephalography in evaluating patients with dementia, while cisternography contributes complementary information."}
{"id": "PMID:1084494", "title": "Cerebrospinal fluid T and B lymphocyte kinetics related to exacerbations of multiple sclerosis.", "content": "Cerebrospinal fluid T and B lymphocyte populations were identified in 21 patients with relapsing and 10 patients with stable multiple sclerosis. The results indicate that the percentage of T cell is largest in the acute stage of a relapse (65 percent) and diminishes by the second week (41 percent). Conversely, the stable patients had a lower percentage of T cells (30 percent) and a relatively high percentage of nonreacting cells (66 percent). The proportion of T cells in the cerebrospinal fluid varies directly with the clinical activity of the disorder, further implicating cellular immune mechanisms in the pathogenesis of multiple sclerosis.", "contents": "Cerebrospinal fluid T and B lymphocyte kinetics related to exacerbations of multiple sclerosis. Cerebrospinal fluid T and B lymphocyte populations were identified in 21 patients with relapsing and 10 patients with stable multiple sclerosis. The results indicate that the percentage of T cell is largest in the acute stage of a relapse (65 percent) and diminishes by the second week (41 percent). Conversely, the stable patients had a lower percentage of T cells (30 percent) and a relatively high percentage of nonreacting cells (66 percent). The proportion of T cells in the cerebrospinal fluid varies directly with the clinical activity of the disorder, further implicating cellular immune mechanisms in the pathogenesis of multiple sclerosis."}
{"id": "PMID:1084495", "title": "Significance of Purkinje cell density in seizure suppresion by chronic cerebellar stimulation.", "content": "Cerebellar biopsy specimens were obtained at the time of cerebellar electrode installation in three epileptic patients. Cerebellar autopsy specimens also were examined from four epileptic patients and from five patients without epilepsy or neurologic disease. All specimens from seizure patients showed isomorphic gliosis of the cerebellar cortices. Significantly lower Purkinje cell densities were found in epileptic patients as compared with nonepileptic control patients. Our two epileptic patients showing marked Purkinje cell loss before cerebellar stimualtaion appeared to have better seizure control during stimulation than the patient who had only mild reduction in Purkinje cell density. These data suggest that augmentation of Purkinje cell inhibitory discharges in not the dominant mechanism for seizure suppression during cerebellar stimulation. Comparison of cerebellar specimens obtained during electrode installation with those obtained later during electrode revisions may have been prognostic significance.", "contents": "Significance of Purkinje cell density in seizure suppresion by chronic cerebellar stimulation. Cerebellar biopsy specimens were obtained at the time of cerebellar electrode installation in three epileptic patients. Cerebellar autopsy specimens also were examined from four epileptic patients and from five patients without epilepsy or neurologic disease. All specimens from seizure patients showed isomorphic gliosis of the cerebellar cortices. Significantly lower Purkinje cell densities were found in epileptic patients as compared with nonepileptic control patients. Our two epileptic patients showing marked Purkinje cell loss before cerebellar stimualtaion appeared to have better seizure control during stimulation than the patient who had only mild reduction in Purkinje cell density. These data suggest that augmentation of Purkinje cell inhibitory discharges in not the dominant mechanism for seizure suppression during cerebellar stimulation. Comparison of cerebellar specimens obtained during electrode installation with those obtained later during electrode revisions may have been prognostic significance."}
{"id": "PMID:1084496", "title": "Myoclonus after 5-hydroxytryptophan in rats with lesions of indoleamine neurons in the central nervous system.", "content": "A myoclonic syndrome consisting of tremor, myoclonus, and seizures was produced following the systemic administration of 5-hydroxytryptophan to adult rats previously given intracisternal injections of 5,7-dihydroxytryptamine and systemic desmethylimipramine, but not in their controls. This behavioral response was blocked by pretreatment with the putative serotonin receptor blocking agents methysergide, lysergic acid diethylamide, and bromolysergic and diethylamide, as well as centrally effective doses of the aromatic amino acid decarboxylase inhibitor Ro4-4602. Blockers of receptors of other neurotransmitters had little effect. This neurologic response in the adult rat may be relevant to some forms of clinical myoclonus and may be useful in testing potential agonists and antagonists of serotonin receptors in the mammalian central nervous system.", "contents": "Myoclonus after 5-hydroxytryptophan in rats with lesions of indoleamine neurons in the central nervous system. A myoclonic syndrome consisting of tremor, myoclonus, and seizures was produced following the systemic administration of 5-hydroxytryptophan to adult rats previously given intracisternal injections of 5,7-dihydroxytryptamine and systemic desmethylimipramine, but not in their controls. This behavioral response was blocked by pretreatment with the putative serotonin receptor blocking agents methysergide, lysergic acid diethylamide, and bromolysergic and diethylamide, as well as centrally effective doses of the aromatic amino acid decarboxylase inhibitor Ro4-4602. Blockers of receptors of other neurotransmitters had little effect. This neurologic response in the adult rat may be relevant to some forms of clinical myoclonus and may be useful in testing potential agonists and antagonists of serotonin receptors in the mammalian central nervous system."}
{"id": "PMID:1084497", "title": "Immunologic characterization of cerebrospinal fluid lymphocytes: preliminary report.", "content": "Cellular immunocompetence of cerebrospinal fluid lymphocytes was investigated in several neurologic diseases. Microtechniques were developed to enable determination of E-rosetting capacity and phytohemagglutinin responsiveness of scant numbers of cells present in the cerebrospinal fluid specimens studied. Although most individuals had phytohemagglutinin-responsive cells in their CSF, reactivity was somewhat less than that found simultaneously in their blood. Three of eight patients had comparable percentages of E rosettes in their blood and CSF. Int the remainder, the values differed significantly. Although preliminary, these result illustrate a new approach to immunologic characterization of CSF lymphocytes in diseases.", "contents": "Immunologic characterization of cerebrospinal fluid lymphocytes: preliminary report. Cellular immunocompetence of cerebrospinal fluid lymphocytes was investigated in several neurologic diseases. Microtechniques were developed to enable determination of E-rosetting capacity and phytohemagglutinin responsiveness of scant numbers of cells present in the cerebrospinal fluid specimens studied. Although most individuals had phytohemagglutinin-responsive cells in their CSF, reactivity was somewhat less than that found simultaneously in their blood. Three of eight patients had comparable percentages of E rosettes in their blood and CSF. Int the remainder, the values differed significantly. Although preliminary, these result illustrate a new approach to immunologic characterization of CSF lymphocytes in diseases."}
{"id": "PMID:1084498", "title": "[Progress of clinical pharmacology in essential headaches].", "content": "By testing venomotor receptors (venous-constriction test), it was possible to show a potentiation of the venous constriction effect of serotonin by methysergide and ergotamine. This 5-HT potentiation was observed only when these drugs were sampled in concentrations similar to those in therapeutic use. When ergotamine and methysergide are sampled in concentrations higher than those used in clinical practice, the expected 5-HT antagonist effect is clearly evident. We could therefore conclude that methysergide and ergotamine can be considered pro-serotonin and not anti-serotonin drugs. The pro-serotonin effect of methysergide and ergotamine might take place peripherally on vasomotor or sensitive receptors and/or at a CNS level, making up for the lack of serotonin which, according to the central theory of essential headache, provokes the painful cephalic syndrome.", "contents": "[Progress of clinical pharmacology in essential headaches]. By testing venomotor receptors (venous-constriction test), it was possible to show a potentiation of the venous constriction effect of serotonin by methysergide and ergotamine. This 5-HT potentiation was observed only when these drugs were sampled in concentrations similar to those in therapeutic use. When ergotamine and methysergide are sampled in concentrations higher than those used in clinical practice, the expected 5-HT antagonist effect is clearly evident. We could therefore conclude that methysergide and ergotamine can be considered pro-serotonin and not anti-serotonin drugs. The pro-serotonin effect of methysergide and ergotamine might take place peripherally on vasomotor or sensitive receptors and/or at a CNS level, making up for the lack of serotonin which, according to the central theory of essential headache, provokes the painful cephalic syndrome."}
{"id": "PMID:1084499", "title": "[Case of reduced platelet adhesion with a slight P.T.A. plasma deficit].", "content": "A case of vascular deficit associated with P.T.A. plasma deficit of hereditary-familiar character has been studied hand described.", "contents": "[Case of reduced platelet adhesion with a slight P.T.A. plasma deficit]. A case of vascular deficit associated with P.T.A. plasma deficit of hereditary-familiar character has been studied hand described."}
{"id": "PMID:1084500", "title": "[Importance of chemical mediators for the pathogenesis and treatment of hemicrania].", "content": "The vascular mechanism of migraine attacks--intracranial vasoconstriction during the prodromal stage and passive vasodilatation of extracranial arteries during the painful phase--has been confirmed by modern methods. In addition, number of humoral factors--plasmakinin, serotonin, histamine--intervene in the chain of events that culminates in migraine headache. In addition to these three humoral mediators, the part played by tyramine in migraine of alimentary origin, the precipitating effect of oestrogen decrease in menstrual migraine and the hypothetical role of prostaglandins and prolactin are discussed. The multifactorial mechanism of migraine accounts for the very variable profiles of effective anti-migraine drugs.", "contents": "[Importance of chemical mediators for the pathogenesis and treatment of hemicrania]. The vascular mechanism of migraine attacks--intracranial vasoconstriction during the prodromal stage and passive vasodilatation of extracranial arteries during the painful phase--has been confirmed by modern methods. In addition, number of humoral factors--plasmakinin, serotonin, histamine--intervene in the chain of events that culminates in migraine headache. In addition to these three humoral mediators, the part played by tyramine in migraine of alimentary origin, the precipitating effect of oestrogen decrease in menstrual migraine and the hypothetical role of prostaglandins and prolactin are discussed. The multifactorial mechanism of migraine accounts for the very variable profiles of effective anti-migraine drugs."}
{"id": "PMID:1084501", "title": "[Transcutaneous nervous stimulation in the treatment of hemicrania and other forms of headache].", "content": "Transcutaneous administration of a slightly painful electrical stimulus was employed in the management of migraine and other forms of headache in 35 patients with various pictures of pain. Stimulation during crisis led to its total disappearance in 6 subjects with migraine. A relatively high percentage of successes was noted in the remaining patients, with persistence up to 8 months. The theory of \"gate control\" is cited to explain this effect. Stress is laid on the particular suitability of the method in the management of chronic headache, since it is not associated with either dependence or toxic medicamentous effects.", "contents": "[Transcutaneous nervous stimulation in the treatment of hemicrania and other forms of headache]. Transcutaneous administration of a slightly painful electrical stimulus was employed in the management of migraine and other forms of headache in 35 patients with various pictures of pain. Stimulation during crisis led to its total disappearance in 6 subjects with migraine. A relatively high percentage of successes was noted in the remaining patients, with persistence up to 8 months. The theory of \"gate control\" is cited to explain this effect. Stress is laid on the particular suitability of the method in the management of chronic headache, since it is not associated with either dependence or toxic medicamentous effects."}
{"id": "PMID:1084503", "title": "Localization of E and EAC binding lymphocytes in human tonsils.", "content": "The localization of T and B lymphocytes in frozen sections of human tonsils was determined using E and EAC binding techniques. It was revealed that there were thymus-dependent areas with E binding lymphocytes in peri- and interfollicular zones. EAC binding lymphocytes were found mainly in the follicles. This suggests that B cells are produced in germinal centres. The present experiment confirmed that human tonsils contain two different kinds of lymphocytes demonstrating selective localizations in the tissues as similar to the lymph nodes.", "contents": "Localization of E and EAC binding lymphocytes in human tonsils. The localization of T and B lymphocytes in frozen sections of human tonsils was determined using E and EAC binding techniques. It was revealed that there were thymus-dependent areas with E binding lymphocytes in peri- and interfollicular zones. EAC binding lymphocytes were found mainly in the follicles. This suggests that B cells are produced in germinal centres. The present experiment confirmed that human tonsils contain two different kinds of lymphocytes demonstrating selective localizations in the tissues as similar to the lymph nodes."}
{"id": "PMID:1084508", "title": "[Blood and cerebrospinal fluid B and T lymphocytes in mumps-meningitis (author's transl)].", "content": "20 children, 12 boys and 8 girls, with mumps-meningitis have been investigated during the acute stage of illness. Immunoglobulin-concentration, B and T cell ratios were determined in venous blood and cerebrospinal fluid. In the blood no correlation between the percentage of B cells and immunoglobulin-levels, or the course of illness could be observed. Total protein level as well as cell count, the concentration of glucose and chloride were determined in the cerebrospinal fluid at the same time. No correlation between the percentage of B cells and immunoglobulin-levels could be observed in cerebrospinal fluid. No difference in the number of B and T cells was observed between blood and cerebrospinal fluid. These results demonstrate, that the lymphocytes in cerebrospinal fluid in meningitis possess the same surface markers and show about the same B to T cell ratio as it is found on the lymphocytes of blood.", "contents": "[Blood and cerebrospinal fluid B and T lymphocytes in mumps-meningitis (author's transl)]. 20 children, 12 boys and 8 girls, with mumps-meningitis have been investigated during the acute stage of illness. Immunoglobulin-concentration, B and T cell ratios were determined in venous blood and cerebrospinal fluid. In the blood no correlation between the percentage of B cells and immunoglobulin-levels, or the course of illness could be observed. Total protein level as well as cell count, the concentration of glucose and chloride were determined in the cerebrospinal fluid at the same time. No correlation between the percentage of B cells and immunoglobulin-levels could be observed in cerebrospinal fluid. No difference in the number of B and T cells was observed between blood and cerebrospinal fluid. These results demonstrate, that the lymphocytes in cerebrospinal fluid in meningitis possess the same surface markers and show about the same B to T cell ratio as it is found on the lymphocytes of blood."}
{"id": "PMID:1084512", "title": "Effects of Newcastle disease virus (NDV) on infection of adult mice with the Thogoto-like Ar-126 arbovirus.", "content": "Inoculation of mice with Newcastle disease virus (NDV) alters the course of infection with the Thogoto-like arbovirus Tho-Ar-126. The Tho-Ar-126 content of liver, spleen, and lymph nodes was approximately 10 times greater in mice treated with NDV 24 h before infection; the mortality was somewhat increased, but liver damage (as indicated by serum transaminase levels) did not seem to be potentiated. Lymphocytopenia was observed in NDV-inoculated mice, and in the spleen and lymph nodes the proportion of lymphocytes susceptible to lysis by anti-theta (a marker for thymus-derived lymphocytes) was markedly decreased in these animals. This suggests that NDV potentiates infection by Tho-Ar-126 through its action on thymus-derived lymphocytes.", "contents": "Effects of Newcastle disease virus (NDV) on infection of adult mice with the Thogoto-like Ar-126 arbovirus. Inoculation of mice with Newcastle disease virus (NDV) alters the course of infection with the Thogoto-like arbovirus Tho-Ar-126. The Tho-Ar-126 content of liver, spleen, and lymph nodes was approximately 10 times greater in mice treated with NDV 24 h before infection; the mortality was somewhat increased, but liver damage (as indicated by serum transaminase levels) did not seem to be potentiated. Lymphocytopenia was observed in NDV-inoculated mice, and in the spleen and lymph nodes the proportion of lymphocytes susceptible to lysis by anti-theta (a marker for thymus-derived lymphocytes) was markedly decreased in these animals. This suggests that NDV potentiates infection by Tho-Ar-126 through its action on thymus-derived lymphocytes."}
{"id": "PMID:1084523", "title": "Transcutaneous electrical nerve stimulation to relieve pain.", "content": "As a therapeutic technique, transcutaneous electrical nerve stimulation is in its infancy, but the instrumentation is rapidly becoming more sophisticated. Mindful of precautions and of the practical limitations of the technique, the practicing physician may find it worthy of serious consideration, particularly in relieving pain on a short-term basis.", "contents": "Transcutaneous electrical nerve stimulation to relieve pain. As a therapeutic technique, transcutaneous electrical nerve stimulation is in its infancy, but the instrumentation is rapidly becoming more sophisticated. Mindful of precautions and of the practical limitations of the technique, the practicing physician may find it worthy of serious consideration, particularly in relieving pain on a short-term basis."}
{"id": "PMID:1084524", "title": "Perinatal infection: diagnosis, treatment, and prevention.", "content": "The majority of clinically recognizable acute infections in the neonate are bacterial. Such infections may be acquired from the mother prior to or at birth or from environmental sources. Because of the limited ability of neonates--especially those born prematurely--to express symptoms, even minor deviations from normal behavior should suggest bacterial disease. Chronic congenital and perinatal infections, unlike acute bacterial disease, are generally asymptomatic in mother and neonate and may remain latent or subclinically active in host tissue for prolonged periods, possibly causing insidious injury to the central nervous and perceptual systems. When overt, these infections almost invariably cause mental or perceptual handicaps or both. In view of the significant mortality and morbidity associated with either acute or chronic infections, diagnosis and treatment should be aggressive.", "contents": "Perinatal infection: diagnosis, treatment, and prevention. The majority of clinically recognizable acute infections in the neonate are bacterial. Such infections may be acquired from the mother prior to or at birth or from environmental sources. Because of the limited ability of neonates--especially those born prematurely--to express symptoms, even minor deviations from normal behavior should suggest bacterial disease. Chronic congenital and perinatal infections, unlike acute bacterial disease, are generally asymptomatic in mother and neonate and may remain latent or subclinically active in host tissue for prolonged periods, possibly causing insidious injury to the central nervous and perceptual systems. When overt, these infections almost invariably cause mental or perceptual handicaps or both. In view of the significant mortality and morbidity associated with either acute or chronic infections, diagnosis and treatment should be aggressive."}
{"id": "PMID:1084525", "title": "Plasma natriuretic activity in oedematous states.", "content": "Accumulating evidence suggests that extracellular volume (ECV) may be governed in part by a natriuretic hormone. To study its possible role in oedema formation plasma fractions IV from patients with the nephrotic syndrome, with cirrhosis of the liver, and with idiopathic oedema were studied for their effects on frog skin Na-transport and on rat renal Na-excretion. Plasma fractions IV from ECV-expanded healthy subjects and patients with aldosteronoma significantly inhibited PD and SCC and in the rat increased urinary flow, CH29, and UNaV. Neither antinatriferic nor natriuretic activities were observed in patients with the nephrotic syndrome or liver cirrhosis. In patients with idiopathic oedema recurrent episodes of fluid retention up to 9 percent of b.wt. followed by spontaneous natriuresis were well correlated with antinatriferic plasma activity. The results suggest that ECV in healthy subjects may be governed in part by a natriuretic hormone which is absent in ECV-expansion due to oedema. However, this mechanism may operate appropriately, though at an elevated threshold, in patients with idiopathic oedema.", "contents": "Plasma natriuretic activity in oedematous states. Accumulating evidence suggests that extracellular volume (ECV) may be governed in part by a natriuretic hormone. To study its possible role in oedema formation plasma fractions IV from patients with the nephrotic syndrome, with cirrhosis of the liver, and with idiopathic oedema were studied for their effects on frog skin Na-transport and on rat renal Na-excretion. Plasma fractions IV from ECV-expanded healthy subjects and patients with aldosteronoma significantly inhibited PD and SCC and in the rat increased urinary flow, CH29, and UNaV. Neither antinatriferic nor natriuretic activities were observed in patients with the nephrotic syndrome or liver cirrhosis. In patients with idiopathic oedema recurrent episodes of fluid retention up to 9 percent of b.wt. followed by spontaneous natriuresis were well correlated with antinatriferic plasma activity. The results suggest that ECV in healthy subjects may be governed in part by a natriuretic hormone which is absent in ECV-expansion due to oedema. However, this mechanism may operate appropriately, though at an elevated threshold, in patients with idiopathic oedema."}
{"id": "PMID:1084526", "title": "Occult blood loss and iron balance in chronic renal failure.", "content": "In 38 patients with chronic renal failure total daily blood loss and intestinal iron absorption were determined by means of a whole-body counter and radioiron. Bone marrow iron content was measured histochemically. Mean daily blood loss (corrected for laboratory blood sampling) and iron absorption were significantly higher than in normal controls. Bone marrow iron content was reduced in 46% of the patients. The results prove that gastrointestinal blood loss in patients with chronic renal failure is of such magnitude that negative iron balance results.", "contents": "Occult blood loss and iron balance in chronic renal failure. In 38 patients with chronic renal failure total daily blood loss and intestinal iron absorption were determined by means of a whole-body counter and radioiron. Bone marrow iron content was measured histochemically. Mean daily blood loss (corrected for laboratory blood sampling) and iron absorption were significantly higher than in normal controls. Bone marrow iron content was reduced in 46% of the patients. The results prove that gastrointestinal blood loss in patients with chronic renal failure is of such magnitude that negative iron balance results."}
{"id": "PMID:1084527", "title": "Ontogeny of murine B lymphocytes: sequence of B-cell differentiation from surface-immunoglobulin-negative precursors to plasma cells.", "content": "Among bone-marrow-derived (B) lymphocytes exist subpopulations of cells that can be induced to express the markers: surface immunoglobulin (Ig), the antigen associated with the immune response gene (Ia), and the receptor for the third complement component (CR). Inducible cells for the first two markers are found in bone marrow, and inducible cells for all three are in spleen. Experiments were designed to determine whether induction involves a single precursor cell population that on triggering with lipopolysaccharide expresses all three surface markers, or three separate precursor cell populations each of which expresses a single marker. Specific B cell subpopulations were eliminated by treatment with anti-Ig or anti-Ia and complement, or by rosette formation with erythrocytes-antibody-complement followed by differential centrifugation, and surviving cells were subsequently tested for inducibility of the three B cell markers. After anti-Ig cytolysis only Ig, but not Ia and CR, could be induced, implying that the Ia- and the CR-inducible cells are Ig+. Similarly, after anti-Ia cytolysis Ig and Ia but not CR could be induced. Thus, CR-inducible cells must have the Ig+Ia+ phenotype. Elimination of CR+ cells did not affect the induction of Ig, Ia, or CR from their precursors. None of the three elimination experiments affected the conversion of prothymocytes (Thy-1-) to thymocytes (Thy-1+). From these results we propose the hypothesis that the differentiation of B lymphocytes proceeds through at least four distinct stages characterized by the following phenotypes: Ig-Ia-CR- leads to Ig+Ia-CR- leads to Ig+Ia+CR- leads to Ig+Ia+CR+.", "contents": "Ontogeny of murine B lymphocytes: sequence of B-cell differentiation from surface-immunoglobulin-negative precursors to plasma cells. Among bone-marrow-derived (B) lymphocytes exist subpopulations of cells that can be induced to express the markers: surface immunoglobulin (Ig), the antigen associated with the immune response gene (Ia), and the receptor for the third complement component (CR). Inducible cells for the first two markers are found in bone marrow, and inducible cells for all three are in spleen. Experiments were designed to determine whether induction involves a single precursor cell population that on triggering with lipopolysaccharide expresses all three surface markers, or three separate precursor cell populations each of which expresses a single marker. Specific B cell subpopulations were eliminated by treatment with anti-Ig or anti-Ia and complement, or by rosette formation with erythrocytes-antibody-complement followed by differential centrifugation, and surviving cells were subsequently tested for inducibility of the three B cell markers. After anti-Ig cytolysis only Ig, but not Ia and CR, could be induced, implying that the Ia- and the CR-inducible cells are Ig+. Similarly, after anti-Ia cytolysis Ig and Ia but not CR could be induced. Thus, CR-inducible cells must have the Ig+Ia+ phenotype. Elimination of CR+ cells did not affect the induction of Ig, Ia, or CR from their precursors. None of the three elimination experiments affected the conversion of prothymocytes (Thy-1-) to thymocytes (Thy-1+). From these results we propose the hypothesis that the differentiation of B lymphocytes proceeds through at least four distinct stages characterized by the following phenotypes: Ig-Ia-CR- leads to Ig+Ia-CR- leads to Ig+Ia+CR- leads to Ig+Ia+CR+."}
{"id": "PMID:1084529", "title": "Adjuvant arthritis in T lymphocyte depleted rats.", "content": "Markedly T lymphocyte depleted rats were prepared by thymectomy, irradiation, and repopulation by bone marrow hematopoietic and lymphoid cells. Such rats had persistent T lymphopenia of about 20% of normal. When T depleted and normal rats were injected with adjuvant, all animals developed arthritis but with slightly less severity in the T depleted animals. Such experiments, and other observations, suggest a complex immunological mechanism in the pathogenesis of adjuvant arthritis in the rat.", "contents": "Adjuvant arthritis in T lymphocyte depleted rats. Markedly T lymphocyte depleted rats were prepared by thymectomy, irradiation, and repopulation by bone marrow hematopoietic and lymphoid cells. Such rats had persistent T lymphopenia of about 20% of normal. When T depleted and normal rats were injected with adjuvant, all animals developed arthritis but with slightly less severity in the T depleted animals. Such experiments, and other observations, suggest a complex immunological mechanism in the pathogenesis of adjuvant arthritis in the rat."}
{"id": "PMID:1084530", "title": "Fate of intraintestinal thymocytes labeled with 125iododeoxyuridine or tritiated thymidine.", "content": "A suspension of thymocytes labeled with 125UIdR or 3HTdR was injected into the jejunum of mice. The bulk of the radioactivity disappeared within few hours from the intestine and was recovered principally in the urine. This indicated a very rapid breakdown of labeled thymic cells, reabsorption and subsequent elimination of the tracer in the kidney. In mice injected with cells labeled with 3HTdR, the initial rapid loss of radioactivity was of shorter duration, and slower during the second phase, presumably due to more extensive reutilization and/or prolonged persistence of acid-soluble radioactivity. Pretreatment of the recipients with antibiotics did not significantly reduce the rate of radioactivity loss.", "contents": "Fate of intraintestinal thymocytes labeled with 125iododeoxyuridine or tritiated thymidine. A suspension of thymocytes labeled with 125UIdR or 3HTdR was injected into the jejunum of mice. The bulk of the radioactivity disappeared within few hours from the intestine and was recovered principally in the urine. This indicated a very rapid breakdown of labeled thymic cells, reabsorption and subsequent elimination of the tracer in the kidney. In mice injected with cells labeled with 3HTdR, the initial rapid loss of radioactivity was of shorter duration, and slower during the second phase, presumably due to more extensive reutilization and/or prolonged persistence of acid-soluble radioactivity. Pretreatment of the recipients with antibiotics did not significantly reduce the rate of radioactivity loss."}
{"id": "PMID:1084531", "title": "Biological bases for the integration of appetitive and consummatory grooming behaviors in the cat: a review.", "content": "Cats with pontile lesions, frontal neocortical lesions, and thyroidectomized cats display a dissociation of the appetitive and consummatory components of grooming behavior following tactile stimulation of the body surface, an abnormal behavior which waxes and wanes with the seasons of the year. Tryptophan hydroxylase activity and serotonin levels were significantly decreased in the superior colliculi (but not other brain regions) in cats with pontile lesions or frontal neocortical lesions, but not in thyroidectomized cats. Systemic administration of 5-hydroxytryptophan or monoamine oxidase inhibition plus tryptophan administration abolishes the abnormal grooming behavior in each group of cats, and microinjections of 5-hydroxytryptophan or serotonin into the superior colliculi has the same effect, indicating that the change in a serotonergic system is a critical aspect of the abnormal behavior in cats with lesions and that a serotonergic system may also be involved in the genesis of the abnormal grooming behavior in thyroidectomized cats. Functional inactivation of the serotonergic system by p-chlorophenylalanine, LSD, or serotonin receptor blockade does not induce the abnormal grooming behavior in normal cats, indicating that other factors are involved. Cats with lesions and thyroidectomized cats display a rhythmic dysfunction in the excretion of glucocorticoids and glucocorticoid administration abolishes the abnormal grooming behavior, suggesting that glucocorticoids are the other critical factor. Adrenalectomized cats do not display the abnormal grooming behavior, but when adrenalectomized cats are treated with p-chlorophenylalanine, the abnormal behavior appears. Thus, a serotonergic system in the superior colliculi, operating at some level of glucocorticoid function, is involved in the integration of appetitive and consummatory grooming behaviors.", "contents": "Biological bases for the integration of appetitive and consummatory grooming behaviors in the cat: a review. Cats with pontile lesions, frontal neocortical lesions, and thyroidectomized cats display a dissociation of the appetitive and consummatory components of grooming behavior following tactile stimulation of the body surface, an abnormal behavior which waxes and wanes with the seasons of the year. Tryptophan hydroxylase activity and serotonin levels were significantly decreased in the superior colliculi (but not other brain regions) in cats with pontile lesions or frontal neocortical lesions, but not in thyroidectomized cats. Systemic administration of 5-hydroxytryptophan or monoamine oxidase inhibition plus tryptophan administration abolishes the abnormal grooming behavior in each group of cats, and microinjections of 5-hydroxytryptophan or serotonin into the superior colliculi has the same effect, indicating that the change in a serotonergic system is a critical aspect of the abnormal behavior in cats with lesions and that a serotonergic system may also be involved in the genesis of the abnormal grooming behavior in thyroidectomized cats. Functional inactivation of the serotonergic system by p-chlorophenylalanine, LSD, or serotonin receptor blockade does not induce the abnormal grooming behavior in normal cats, indicating that other factors are involved. Cats with lesions and thyroidectomized cats display a rhythmic dysfunction in the excretion of glucocorticoids and glucocorticoid administration abolishes the abnormal grooming behavior, suggesting that glucocorticoids are the other critical factor. Adrenalectomized cats do not display the abnormal grooming behavior, but when adrenalectomized cats are treated with p-chlorophenylalanine, the abnormal behavior appears. Thus, a serotonergic system in the superior colliculi, operating at some level of glucocorticoid function, is involved in the integration of appetitive and consummatory grooming behaviors."}
{"id": "PMID:1084532", "title": "Pharmacological studies of N,N-dimethylaminoethyl chloroacetate and N,N-dimethylaminoethyl acrylate as inhibitors of choline acetyltransferase in isolated skeletal and smooth muscle preparations.", "content": "Two tertiary amine esters, N,N-dimethylaminoethyl chloroacetate (Cl-DMA) and N,N-dimethylaminoethyl acrylate (acryl-DMA), which have recently been shown to be inhibitors of choline acetyltransferase (ChAc) were investigated to determine their actions in isolated skeletal and smooth muscle preparations. Both compounds caused neuromuscular blockade in indirectly stimulated nerve-muscle preparations (ED50 values of Cl-DMA were 6.9 -42.0 X 10(-4) M and those of acryl-DMA were 1.2-5.8 X 10(-4) M). The blockade was completely or partially reversible after drug washout. A comparison of the ED50 values for neuromuscular blockade with the ID50 values for ChAc inhibition suggested that the acryl-DMA compound might not cause neuromuscular blockade via ChAc inhibition because the potency ratios (ED50/ID50) of Cl-DMA were higher than 1, whereas those of acryl-DMA were equal to or lower than 1. This was borne out by further experiments on isolated neuromuscular preparations which showed that the site of action for acryl-DMA was post-junctional, whereas that for Cl-DMA was prejunctional. In addition, the weak stimulating properties of Cl-DMA and acryl-DMA were investigated in isolated skeletal and smooth muscle. Cl-DMA was shown to be a partial cholinergic agonist, whereas acryl-DMA was a nonspecific stimulant not involving cholinergic receptors. Although both Cl-DMA and acryl-DMA are inhibitors of ChAc, only Cl-DMA appears to have sufficient specificity for use as a possible ChAc inhibitor in vivo.", "contents": "Pharmacological studies of N,N-dimethylaminoethyl chloroacetate and N,N-dimethylaminoethyl acrylate as inhibitors of choline acetyltransferase in isolated skeletal and smooth muscle preparations. Two tertiary amine esters, N,N-dimethylaminoethyl chloroacetate (Cl-DMA) and N,N-dimethylaminoethyl acrylate (acryl-DMA), which have recently been shown to be inhibitors of choline acetyltransferase (ChAc) were investigated to determine their actions in isolated skeletal and smooth muscle preparations. Both compounds caused neuromuscular blockade in indirectly stimulated nerve-muscle preparations (ED50 values of Cl-DMA were 6.9 -42.0 X 10(-4) M and those of acryl-DMA were 1.2-5.8 X 10(-4) M). The blockade was completely or partially reversible after drug washout. A comparison of the ED50 values for neuromuscular blockade with the ID50 values for ChAc inhibition suggested that the acryl-DMA compound might not cause neuromuscular blockade via ChAc inhibition because the potency ratios (ED50/ID50) of Cl-DMA were higher than 1, whereas those of acryl-DMA were equal to or lower than 1. This was borne out by further experiments on isolated neuromuscular preparations which showed that the site of action for acryl-DMA was post-junctional, whereas that for Cl-DMA was prejunctional. In addition, the weak stimulating properties of Cl-DMA and acryl-DMA were investigated in isolated skeletal and smooth muscle. Cl-DMA was shown to be a partial cholinergic agonist, whereas acryl-DMA was a nonspecific stimulant not involving cholinergic receptors. Although both Cl-DMA and acryl-DMA are inhibitors of ChAc, only Cl-DMA appears to have sufficient specificity for use as a possible ChAc inhibitor in vivo."}
{"id": "PMID:1084533", "title": "Principles of therapy in cirrhosis.", "content": "Since progression of certain types of cirrhosis can be prevented by withdrawal of the offending agent or administration of anti-inflammatory drugs, optimal therapy depends on accurate diagnosis of the specific cause. Anticipation and appropriate treatement of complications will allow a more favorable prognosis.", "contents": "Principles of therapy in cirrhosis. Since progression of certain types of cirrhosis can be prevented by withdrawal of the offending agent or administration of anti-inflammatory drugs, optimal therapy depends on accurate diagnosis of the specific cause. Anticipation and appropriate treatement of complications will allow a more favorable prognosis."}
{"id": "PMID:1084534", "title": "[Echoencephalographic determination of normal values of the width of the third ventricle in children].", "content": "The maximum diameter of the third ventricle was ultrasonically determined on 769 children aged 3 to 10, of whom 401 were boys and 368, girls. The empirical range included values between 2.1 and 5.5 mm, the average value being 3.8 mm and the standard deviation, 0.57 mm. Limiting ranges bordering on pathological values are, for the time being, considered to be as follows: 4.5 mm in the third year of life; 5.0 mm in the fourth to fifth years of life; 5.5 mm in the sixth to seventh years of life; and 6.0 mm in the eighth to tenth years of life.", "contents": "[Echoencephalographic determination of normal values of the width of the third ventricle in children]. The maximum diameter of the third ventricle was ultrasonically determined on 769 children aged 3 to 10, of whom 401 were boys and 368, girls. The empirical range included values between 2.1 and 5.5 mm, the average value being 3.8 mm and the standard deviation, 0.57 mm. Limiting ranges bordering on pathological values are, for the time being, considered to be as follows: 4.5 mm in the third year of life; 5.0 mm in the fourth to fifth years of life; 5.5 mm in the sixth to seventh years of life; and 6.0 mm in the eighth to tenth years of life."}
{"id": "PMID:1084537", "title": "Computed tomography: evaluation of the posterior third ventricle.", "content": "The normal computed tomography (CT) appearances of the posterior third ventricle and related structures are presented. Seventy-six patients with lesions directly involving this ventricle were studied by CT, and results of other neuroradiological procedures evaluated. Primary tumors, metastases, hematomas, infarcts, and cysts may be reliably distinguished by CT, particularly if contrast enhancement is utilized. Size and density resolution limit the direct visualization of the aqueduct of Sylvius; stenosis however may be inferred. Small infarcts may be difficult to demonstrate by the use of CT scans without contrast enhancement, yet be shown by isotope scans.", "contents": "Computed tomography: evaluation of the posterior third ventricle. The normal computed tomography (CT) appearances of the posterior third ventricle and related structures are presented. Seventy-six patients with lesions directly involving this ventricle were studied by CT, and results of other neuroradiological procedures evaluated. Primary tumors, metastases, hematomas, infarcts, and cysts may be reliably distinguished by CT, particularly if contrast enhancement is utilized. Size and density resolution limit the direct visualization of the aqueduct of Sylvius; stenosis however may be inferred. Small infarcts may be difficult to demonstrate by the use of CT scans without contrast enhancement, yet be shown by isotope scans."}
{"id": "PMID:1084538", "title": "Enterolith causing bleeding in a patient with Meckel's diverticulum. Angiographic demonstration.", "content": "Angiography demonstrated a bleeding Meckel's diverticulum supplied by tortuous, presumably embryonic branches of the superior mesenteric artery. A large enterolith, partially impacted into a constriction near the center of the diverticulum, caused a bleeding ulcer. The diverticulum contained no gastric mucosa.", "contents": "Enterolith causing bleeding in a patient with Meckel's diverticulum. Angiographic demonstration. Angiography demonstrated a bleeding Meckel's diverticulum supplied by tortuous, presumably embryonic branches of the superior mesenteric artery. A large enterolith, partially impacted into a constriction near the center of the diverticulum, caused a bleeding ulcer. The diverticulum contained no gastric mucosa."}
{"id": "PMID:1084540", "title": "Migration into pseudo-follicles of draining lymph nodes of medullary small thymocytes injected in the mediastinal cavity.", "content": "After an intramediastinal injection of labelled thymic cells, few cells were found in the pseudo-follicles of the draining nodes while the extrafollicular zone of their cortex contained abundant labelled cells (Sainte-Marie and Peng, in press). We proposed that the cells having migrated into the pseudo-follicles were small lymphocytes of the thymic medulla, or medullary small thymocytes, which accounts for about 5% of the thymocyte population. The purpose of the present study was to test the validity of the proposal. Rats received corticosterone injections to destroy, their cortical thymocytes and, thereafter, a dose of 3H-cytidine in order to label the surviving medullary small thymocytes. One hour later, these cells were suspended and injected in the mediastinal cavity of recipients which were killed 3 and 24 hours after the injection. The radio-autographs of the draining, and of the remaining, nodes revealed that most labelled cells, present in the nodes at the 24-hour interval, were situated mostly in the pseudo-follicles. The finding indicates, that, unlike the cortical small thymocytes, the medullary small thymocytes can migrate into pseudo-follicles. This is probably due to the greater motility of the medullary small thymocytes and possibly, to their involvement in the function(s) carried out in the pseudo-follicles.", "contents": "Migration into pseudo-follicles of draining lymph nodes of medullary small thymocytes injected in the mediastinal cavity. After an intramediastinal injection of labelled thymic cells, few cells were found in the pseudo-follicles of the draining nodes while the extrafollicular zone of their cortex contained abundant labelled cells (Sainte-Marie and Peng, in press). We proposed that the cells having migrated into the pseudo-follicles were small lymphocytes of the thymic medulla, or medullary small thymocytes, which accounts for about 5% of the thymocyte population. The purpose of the present study was to test the validity of the proposal. Rats received corticosterone injections to destroy, their cortical thymocytes and, thereafter, a dose of 3H-cytidine in order to label the surviving medullary small thymocytes. One hour later, these cells were suspended and injected in the mediastinal cavity of recipients which were killed 3 and 24 hours after the injection. The radio-autographs of the draining, and of the remaining, nodes revealed that most labelled cells, present in the nodes at the 24-hour interval, were situated mostly in the pseudo-follicles. The finding indicates, that, unlike the cortical small thymocytes, the medullary small thymocytes can migrate into pseudo-follicles. This is probably due to the greater motility of the medullary small thymocytes and possibly, to their involvement in the function(s) carried out in the pseudo-follicles."}
{"id": "PMID:1084543", "title": "[Cold lymphocytotoxins in various pathological situations].", "content": "Cold lymphocytotoxins have been described extensively in many situations. In the present work, this kind of antibody has been found less frequently than previously in some diseases like infectious mononucleosis, rheumato\u00efd arthritis, or kidney transplanted patients. These discrepancies may be due to technical considerations. In contrast, cold lymphocytotoxins, in the present work, have been frequently detected in auto-immune hemolytic anemias with cold agglutinins, and in patients having immuno-deficiencies.", "contents": "[Cold lymphocytotoxins in various pathological situations]. Cold lymphocytotoxins have been described extensively in many situations. In the present work, this kind of antibody has been found less frequently than previously in some diseases like infectious mononucleosis, rheumato\u00efd arthritis, or kidney transplanted patients. These discrepancies may be due to technical considerations. In contrast, cold lymphocytotoxins, in the present work, have been frequently detected in auto-immune hemolytic anemias with cold agglutinins, and in patients having immuno-deficiencies."}
{"id": "PMID:1084544", "title": "[Application of free flow preparative methods for the separation of blood cells].", "content": "Human blood cells can be separated using an apparatus described by Hanning and co-workers (Model FF4 Desaga Heidelberg). The method of free flow preparative electrophoresis uses a specific property of blood cells when subjected to an electric field: their electrophoretic mobility. Three regions of migration can be defined: --a high mobility region (HMR); --a low mobility region (LMR) and --an intermediate mobility region (IMR). After the electrophoretic migration, the various cell fractions are identified. If human leucocytes are subjected to an electric field (40 volts/cm) it is possible to isolate several populations which differ by their membranes electric charge. The repartition of the neutrophiles granulocytes is twofold: --one fraction of high mobility (HMR) and --one fraction of low mobility (LMR). The meaning of these two populations is being discussed. Between these teo fractions is located the lymphocyte fraction which is represented by a single peak of intermediate mobility (IMR). Eosinophiles and monocytes are concentrated in low mobility fraction (LMR). With appropriate migration parameters (80 volts/cm, various component buffers) it is possible to obtain from lymphocytes, purified by density gradient, an electrophoretic separation of T and B lymphocytes population. Histograms obtained from quantitative measures (on collected cell fractions) show an unimodal distribution. When separated cells are characterized by three membranes markers (E. Rosettes, EAC Rosettes and surface immunoglobulins) the electrophoretic heterogeneity of B and T cells is then demonstrated. With E Rosettes (T cells) the majority of T lymphocytes is distributed in HMR and IMR. With EAC Rosettes and membrane immunofluorescence (B cells markers) the majority of B lymphocytes is found in the LMR. Thus an important enrichment of T and B sub-population is obtained, but contamination from one another still remains too important. More selective methods should lead to an improvement of this cell separation.", "contents": "[Application of free flow preparative methods for the separation of blood cells]. Human blood cells can be separated using an apparatus described by Hanning and co-workers (Model FF4 Desaga Heidelberg). The method of free flow preparative electrophoresis uses a specific property of blood cells when subjected to an electric field: their electrophoretic mobility. Three regions of migration can be defined: --a high mobility region (HMR); --a low mobility region (LMR) and --an intermediate mobility region (IMR). After the electrophoretic migration, the various cell fractions are identified. If human leucocytes are subjected to an electric field (40 volts/cm) it is possible to isolate several populations which differ by their membranes electric charge. The repartition of the neutrophiles granulocytes is twofold: --one fraction of high mobility (HMR) and --one fraction of low mobility (LMR). The meaning of these two populations is being discussed. Between these teo fractions is located the lymphocyte fraction which is represented by a single peak of intermediate mobility (IMR). Eosinophiles and monocytes are concentrated in low mobility fraction (LMR). With appropriate migration parameters (80 volts/cm, various component buffers) it is possible to obtain from lymphocytes, purified by density gradient, an electrophoretic separation of T and B lymphocytes population. Histograms obtained from quantitative measures (on collected cell fractions) show an unimodal distribution. When separated cells are characterized by three membranes markers (E. Rosettes, EAC Rosettes and surface immunoglobulins) the electrophoretic heterogeneity of B and T cells is then demonstrated. With E Rosettes (T cells) the majority of T lymphocytes is distributed in HMR and IMR. With EAC Rosettes and membrane immunofluorescence (B cells markers) the majority of B lymphocytes is found in the LMR. Thus an important enrichment of T and B sub-population is obtained, but contamination from one another still remains too important. More selective methods should lead to an improvement of this cell separation."}
{"id": "PMID:1084547", "title": "Suppression and stimulation of immunoglobulin synthesis by agents present in rheumatoid synovial effusions.", "content": "Synovial effusions from 14 patients with rheumatoid arthritis contain factors capable of enhancing and suppressing the production of immunoglobulin when added to normal peripheral lymphocyte cultures in vitro. Utilizing a radioactive immune coprecipitation technique it could be demonstrated that enhancement was observed upon addition of relatively small amounts of synovial fluid, while addition of higher amounts resulted in suppression of immunoglobulin synthesis. Nine of ten non-rheumatoid joint effusions showed neither enhancing nor suppressing activities. The possible relationship of these events to lymphokine production in the joint is discussed.", "contents": "Suppression and stimulation of immunoglobulin synthesis by agents present in rheumatoid synovial effusions. Synovial effusions from 14 patients with rheumatoid arthritis contain factors capable of enhancing and suppressing the production of immunoglobulin when added to normal peripheral lymphocyte cultures in vitro. Utilizing a radioactive immune coprecipitation technique it could be demonstrated that enhancement was observed upon addition of relatively small amounts of synovial fluid, while addition of higher amounts resulted in suppression of immunoglobulin synthesis. Nine of ten non-rheumatoid joint effusions showed neither enhancing nor suppressing activities. The possible relationship of these events to lymphokine production in the joint is discussed."}
{"id": "PMID:1084550", "title": "Estimation of B- and T-lymphocytes in lymphoid tissue by means of photometry and 51Cr labelled marker erythrocytes.", "content": "Some new techniques for estimating B- and T-lymphocytes in lymphoid tissue are introduced. The density of marker erythrocytes on closed chamber incubated sections was estimated by the light transmission in relation to the number of the respective lymphocyte subpopulation, measured on suspensions of eluted lymphocytes. Using 51Cr labelled marker erythrocytes, the number of adsorbed marker erythrocytes per lymphocyte could be estimated from the gamma-irradiation of the section. The number of lymphocytes in the section was determined by the amount of protein in the section compared with the protein amount of a tissue specimen of known wet weight and known lymphocyte content. Furthermore, the number of adsorbed marker erythrocytes per rosette in suspensions of eluted lymphocytes was estimated by means of radioactively labelled marker erythrocytes.", "contents": "Estimation of B- and T-lymphocytes in lymphoid tissue by means of photometry and 51Cr labelled marker erythrocytes. Some new techniques for estimating B- and T-lymphocytes in lymphoid tissue are introduced. The density of marker erythrocytes on closed chamber incubated sections was estimated by the light transmission in relation to the number of the respective lymphocyte subpopulation, measured on suspensions of eluted lymphocytes. Using 51Cr labelled marker erythrocytes, the number of adsorbed marker erythrocytes per lymphocyte could be estimated from the gamma-irradiation of the section. The number of lymphocytes in the section was determined by the amount of protein in the section compared with the protein amount of a tissue specimen of known wet weight and known lymphocyte content. Furthermore, the number of adsorbed marker erythrocytes per rosette in suspensions of eluted lymphocytes was estimated by means of radioactively labelled marker erythrocytes."}
{"id": "PMID:1084549", "title": "[Motor complications during Herpes zoster of the ophtalmic branch of the trigeminal nerve. Electromyographic study of 7 cases (author's transl)].", "content": "It has been recently shown that in addition to ganglia the nerve is involved during Herpes Zoster. Electromyographic investigations of the masseter muscle, were carried out on the side of the skin lesions in 7 cases of Herpes Zoster of the first trigeminal branch. In 4 cases there was a partial denervation, possibly due to a mononeuritis of the motor branch of the trigeminal nerve. It is probable that systematic electromyographic examinations may demonstrate a higher proportion of motor complications during Herpes Zoster than previously accepted.", "contents": "[Motor complications during Herpes zoster of the ophtalmic branch of the trigeminal nerve. Electromyographic study of 7 cases (author's transl)]. It has been recently shown that in addition to ganglia the nerve is involved during Herpes Zoster. Electromyographic investigations of the masseter muscle, were carried out on the side of the skin lesions in 7 cases of Herpes Zoster of the first trigeminal branch. In 4 cases there was a partial denervation, possibly due to a mononeuritis of the motor branch of the trigeminal nerve. It is probable that systematic electromyographic examinations may demonstrate a higher proportion of motor complications during Herpes Zoster than previously accepted."}
{"id": "PMID:1084574", "title": "Extracorporeal irradiation of thoracic duct lymph as immunosuppressive treatment in rheumatoid arthritis.", "content": "Thoracic duct drainage and re-infusion of the irradiated lymph was carried out as immunosuppressive treatment in 2 patients with progressive, therapy-resistant rheumatoid arthritis. In both patients, a marked clinical improvement was achieved even during the first days of treatment. A reduced number of T cells in the blood was seen 3 days after onset of drainage, whereas no significant change in the number of B cells was observed. No recirculation of the infused cells could be detected, nor was the radiation removal of T cells accompanied by rapid proliferation of \"new\" T cells. As clinical improvement and reduction in T cells occurred simultaneously, there is probably a connection between these two events. The beneficial clinical response and the achievement of T cell suppression by thoracic duct drainage--the result of irradiation and re-infusion of irradiated lymph--encourage further clinical trials with this type of treatment in severe therapy-resistant rheumatoid arthritis.", "contents": "Extracorporeal irradiation of thoracic duct lymph as immunosuppressive treatment in rheumatoid arthritis. Thoracic duct drainage and re-infusion of the irradiated lymph was carried out as immunosuppressive treatment in 2 patients with progressive, therapy-resistant rheumatoid arthritis. In both patients, a marked clinical improvement was achieved even during the first days of treatment. A reduced number of T cells in the blood was seen 3 days after onset of drainage, whereas no significant change in the number of B cells was observed. No recirculation of the infused cells could be detected, nor was the radiation removal of T cells accompanied by rapid proliferation of \"new\" T cells. As clinical improvement and reduction in T cells occurred simultaneously, there is probably a connection between these two events. The beneficial clinical response and the achievement of T cell suppression by thoracic duct drainage--the result of irradiation and re-infusion of irradiated lymph--encourage further clinical trials with this type of treatment in severe therapy-resistant rheumatoid arthritis."}
{"id": "PMID:1084575", "title": "Granulocyte reactive antinuclear factors in juvenile rheumatoid arthritis.", "content": "The prevalence of mouse liver reactive antinuclear factors (L-ANF) and human granulocyte reactive antinuclear factors (G-ANF) was determined in sera from 34 age- and sex-matched pairs of patients with juvenile rheumatoid arthritis (JRA) and controls. Neither type of antinuclear factor was found in any of the control sera, whereas of the patient sera, 35% were L-ANF positive and 59% were G-ANF positive. 90% of the G-ANF positive sera were of the IgG class and 50% were able to fix complement. L-ANF was most frequent in female patients. Several types of ANF were associated with the polyarticular form of the disease. Such varying prevalences of ANF with disease types might indicate that JRA is a syndrome rather than a disease. In particular, the complement fixing G-ANF titre was positively correlated with parameters related to disease activity. The great variation in prevalence figures of different ANF types previously reported and the possible clinical significance of these auto-antibodies are discussed.", "contents": "Granulocyte reactive antinuclear factors in juvenile rheumatoid arthritis. The prevalence of mouse liver reactive antinuclear factors (L-ANF) and human granulocyte reactive antinuclear factors (G-ANF) was determined in sera from 34 age- and sex-matched pairs of patients with juvenile rheumatoid arthritis (JRA) and controls. Neither type of antinuclear factor was found in any of the control sera, whereas of the patient sera, 35% were L-ANF positive and 59% were G-ANF positive. 90% of the G-ANF positive sera were of the IgG class and 50% were able to fix complement. L-ANF was most frequent in female patients. Several types of ANF were associated with the polyarticular form of the disease. Such varying prevalences of ANF with disease types might indicate that JRA is a syndrome rather than a disease. In particular, the complement fixing G-ANF titre was positively correlated with parameters related to disease activity. The great variation in prevalence figures of different ANF types previously reported and the possible clinical significance of these auto-antibodies are discussed."}
{"id": "PMID:1084576", "title": "A human syndrome caused by immotile cilia.", "content": "Four subjects who produced immotile sperm were studied. In three of the subjects, who had frequent bronchitis and sinusitis, there was no mucociliary transport, as measured by tracheobronchial clearance. Electron microscopy indicated that cilia from cells of these patients lack dynein arms.", "contents": "A human syndrome caused by immotile cilia. Four subjects who produced immotile sperm were studied. In three of the subjects, who had frequent bronchitis and sinusitis, there was no mucociliary transport, as measured by tracheobronchial clearance. Electron microscopy indicated that cilia from cells of these patients lack dynein arms."}
{"id": "PMID:1084577", "title": "Myocardial infarction in patients less than 45 years old: acute course, angiographic findings, and long-term follow-up.", "content": "The clinical courses of 57 patients who had an acute myocardial infarction when less than 45 years of age were reviewed to identify descriptors which might be useful in selecting candidates for coronary artery surgery. Follow-up for the subgroup of 45 patients seen initially with acute myocardial infarction was 98% (44/45) complete. Those patients referred to the medical center because of complications for specifically for coronary artery surgery following recent infarction were not followed. Average duration of follow-up for survivors without coronary surgery was 45 months and ranged from 10 to 105 months. The hospital mortality for the above group was 16% (7/45). During the follow-up period, 11% (4/37) died, 51% (19/37) had angina, 19% (7/37) had congestive heart failure, 51% (19/37) returned to work, and only 43% (16/37) remained asymptomatic. Angiographic assessment of coronary artery lesions by the Friesinger method indicated moderately severe disease in these young patients. Multiple regression analysis did not reveal a reliable predictor of hospital or late death or subsequent morbidity.", "contents": "Myocardial infarction in patients less than 45 years old: acute course, angiographic findings, and long-term follow-up. The clinical courses of 57 patients who had an acute myocardial infarction when less than 45 years of age were reviewed to identify descriptors which might be useful in selecting candidates for coronary artery surgery. Follow-up for the subgroup of 45 patients seen initially with acute myocardial infarction was 98% (44/45) complete. Those patients referred to the medical center because of complications for specifically for coronary artery surgery following recent infarction were not followed. Average duration of follow-up for survivors without coronary surgery was 45 months and ranged from 10 to 105 months. The hospital mortality for the above group was 16% (7/45). During the follow-up period, 11% (4/37) died, 51% (19/37) had angina, 19% (7/37) had congestive heart failure, 51% (19/37) returned to work, and only 43% (16/37) remained asymptomatic. Angiographic assessment of coronary artery lesions by the Friesinger method indicated moderately severe disease in these young patients. Multiple regression analysis did not reveal a reliable predictor of hospital or late death or subsequent morbidity."}
{"id": "PMID:1084581", "title": "Bilateral renal cortical necrosis: a report of 2 cases.", "content": "Two cases of renal cortical necrosis, one of which occurred after an obstetric complication (abruptio placentae) and the other after postpartum haemorrhage, are described. The diagnosis was made by percutaneous renal biopsy, intravenous pyelography and selective nephro-angiography. Immunofluorescence studies of the kidney showed no abnormality in one patient, but showed the presence of IgM in the glomerular basement membrane in the second patient. Hypotension was not observed when anuria occurred. Both patients survived. The importance of prolonged haemodialysis is stressed, since one patient was oliguric for 57 days and required intermittent haemodialysis for 5 months, while the second patient was oliguric for 17 days, required haemodialysis for 5 months and now has established hypertension.", "contents": "Bilateral renal cortical necrosis: a report of 2 cases. Two cases of renal cortical necrosis, one of which occurred after an obstetric complication (abruptio placentae) and the other after postpartum haemorrhage, are described. The diagnosis was made by percutaneous renal biopsy, intravenous pyelography and selective nephro-angiography. Immunofluorescence studies of the kidney showed no abnormality in one patient, but showed the presence of IgM in the glomerular basement membrane in the second patient. Hypotension was not observed when anuria occurred. Both patients survived. The importance of prolonged haemodialysis is stressed, since one patient was oliguric for 57 days and required intermittent haemodialysis for 5 months, while the second patient was oliguric for 17 days, required haemodialysis for 5 months and now has established hypertension."}
{"id": "PMID:1084591", "title": "[Observations in two cases of primary fibrosarcoma of the lung (author's transl)].", "content": "Two cases of primary fibrosarcoma of the lung are reported. The different clinical findings as well as the different courses in both patients reveal problems in diagnosis and treatment of this rare pulmonary lesion. The diagnostic aspects are given and discussed with regards to bronchial carcinoma. It is believed that lobectomy is the rational treatment of primary fibrosarcoma although pneumonectomy must be considered in the indifferentiated typ. The doubtful prognosis requires an exact postoperative follow up in order to have the change of early surgical treatment or irradiation if metastasis or recurrence of the tumor occurs.", "contents": "[Observations in two cases of primary fibrosarcoma of the lung (author's transl)]. Two cases of primary fibrosarcoma of the lung are reported. The different clinical findings as well as the different courses in both patients reveal problems in diagnosis and treatment of this rare pulmonary lesion. The diagnostic aspects are given and discussed with regards to bronchial carcinoma. It is believed that lobectomy is the rational treatment of primary fibrosarcoma although pneumonectomy must be considered in the indifferentiated typ. The doubtful prognosis requires an exact postoperative follow up in order to have the change of early surgical treatment or irradiation if metastasis or recurrence of the tumor occurs."}
{"id": "PMID:1084592", "title": "[Bronchial carcinoids (author's transl)].", "content": "Of 35 patients with bronchial carcinoids seen at the Kantonsspital Z\u00fcrich during 16 years, 33 underwent an operative resection. The endoscopic resection is not recommended because of the risk of hemorrhage recurrence, and that this operation results in only partial removal of carcinoids which develop extrabronchially. It is concluded that bronchial carcinoids are potentially malignant tumours and that resection should be most radical if they show recurrence, invasive growth or metastases. Controls are necessary for a long period of time because even in presence of metastases the growth is very slow.", "contents": "[Bronchial carcinoids (author's transl)]. Of 35 patients with bronchial carcinoids seen at the Kantonsspital Z\u00fcrich during 16 years, 33 underwent an operative resection. The endoscopic resection is not recommended because of the risk of hemorrhage recurrence, and that this operation results in only partial removal of carcinoids which develop extrabronchially. It is concluded that bronchial carcinoids are potentially malignant tumours and that resection should be most radical if they show recurrence, invasive growth or metastases. Controls are necessary for a long period of time because even in presence of metastases the growth is very slow."}
{"id": "PMID:1084593", "title": "[Leiomyoma of the esophagus (author's transl)].", "content": "Benign neoplasms of the esophagus are rare. According to Linder and Gr\u00f6zinger (1968) they come up to about 0,5-2% of all esophageal tumors. Among new growths of mesenchymal origin leiomyomas are dominant in 75-85%. In nearly the half of the concerned patients symptoms occur, indicating operative intervention. At the Surgical Department of the RWTH Aachen besides 58 patients with esophageal cancer only 4 patients suffering from benign tumors have been operated on between 1967 and 1975. Histologically three times the diagnosis of a leiomyoma could be established. In one female, a preoperatively simulated leiomyoma was verified as an intramural cyst of bronchogenic origin. Thoracotomy and enucleation or extirpation are the preferable methods of treatment. Postoperatively no essential complications were seen. A clinically silent fistula healed up spontaneously.", "contents": "[Leiomyoma of the esophagus (author's transl)]. Benign neoplasms of the esophagus are rare. According to Linder and Gr\u00f6zinger (1968) they come up to about 0,5-2% of all esophageal tumors. Among new growths of mesenchymal origin leiomyomas are dominant in 75-85%. In nearly the half of the concerned patients symptoms occur, indicating operative intervention. At the Surgical Department of the RWTH Aachen besides 58 patients with esophageal cancer only 4 patients suffering from benign tumors have been operated on between 1967 and 1975. Histologically three times the diagnosis of a leiomyoma could be established. In one female, a preoperatively simulated leiomyoma was verified as an intramural cyst of bronchogenic origin. Thoracotomy and enucleation or extirpation are the preferable methods of treatment. Postoperatively no essential complications were seen. A clinically silent fistula healed up spontaneously."}
{"id": "PMID:1084594", "title": "[Surgery for coarctation of the aorta in the first year of life (author's transl)].", "content": "A new surgical repair of coarcation of the aorta is reported on 7 infants in the first year of life. Hereby the subclavian artery is used as a modified patch for the coarctation and utilised for re-establishing aortic continuity. The results and advantages are presented and discussed.", "contents": "[Surgery for coarctation of the aorta in the first year of life (author's transl)]. A new surgical repair of coarcation of the aorta is reported on 7 infants in the first year of life. Hereby the subclavian artery is used as a modified patch for the coarctation and utilised for re-establishing aortic continuity. The results and advantages are presented and discussed."}
{"id": "PMID:1084595", "title": "[Penetrating shot injury of the intern carotid artery (author's transl)].", "content": "The case of a young male patient with gun-shot-trauma of the internal right carotid artery is reported. The primary arterial reconstruction in the hemiparetic and unconscious man was successfully done. The problems of the carotid shot trauma - especially indication, time of reparation, kind of operation and the intraluminary shunt - are discussed.", "contents": "[Penetrating shot injury of the intern carotid artery (author's transl)]. The case of a young male patient with gun-shot-trauma of the internal right carotid artery is reported. The primary arterial reconstruction in the hemiparetic and unconscious man was successfully done. The problems of the carotid shot trauma - especially indication, time of reparation, kind of operation and the intraluminary shunt - are discussed."}
{"id": "PMID:1084597", "title": "A new cannulation method for isolated mitral valve surgery--\"apicoaortic-pa\" cannulation.", "content": "The present paper describes experimental and clinical studies of a new method \"Apicoaortic-PA\" cannulation for mitral valve surgery. Our experimental study showed that this method was more rapid and more physiological for cardiopulmonary bypass. We used this technique in 55 cases of isolated mitral valve surgery with successful results. Our general philosophy of surgical approach to the mitral valve diseases is also discussed. We advocate the utilization of the \"Apicoaortic Pulmonary Artery\" cannulation method for clinical use in isolated mitral valve surgery through the left thoracotomy.", "contents": "A new cannulation method for isolated mitral valve surgery--\"apicoaortic-pa\" cannulation. The present paper describes experimental and clinical studies of a new method \"Apicoaortic-PA\" cannulation for mitral valve surgery. Our experimental study showed that this method was more rapid and more physiological for cardiopulmonary bypass. We used this technique in 55 cases of isolated mitral valve surgery with successful results. Our general philosophy of surgical approach to the mitral valve diseases is also discussed. We advocate the utilization of the \"Apicoaortic Pulmonary Artery\" cannulation method for clinical use in isolated mitral valve surgery through the left thoracotomy."}
{"id": "PMID:1084598", "title": "[Cor triatriatum: total correction in an infant using normothermic inflow occlusion (author's transl)].", "content": "Successful correction of cor triatiatum in a 3-month-old infant is presented. The diagnosis was established preoperatively by separate angiocardiographic visualization of the two left atrial compartments. To our knowledge all successful total corrections were done under cardiopulmonary bypass. In this case, the abnormal diaphragm in the left atrium was resected under normothermic inflow occlusion. We believe that the technically very simple resection of the diaphragm does not necessitate the use of extracorporeal circulation in an infant, provided the diagnosis can be made beyond any doubt before the operation.", "contents": "[Cor triatriatum: total correction in an infant using normothermic inflow occlusion (author's transl)]. Successful correction of cor triatiatum in a 3-month-old infant is presented. The diagnosis was established preoperatively by separate angiocardiographic visualization of the two left atrial compartments. To our knowledge all successful total corrections were done under cardiopulmonary bypass. In this case, the abnormal diaphragm in the left atrium was resected under normothermic inflow occlusion. We believe that the technically very simple resection of the diaphragm does not necessitate the use of extracorporeal circulation in an infant, provided the diagnosis can be made beyond any doubt before the operation."}
{"id": "PMID:1084599", "title": "[Clinical experiences with cardioplegic injection in open heart surgery (author's transl)].", "content": "Two groups of 50 patients each who had undergone valve replacement with extracorporeal circulation, were examined. In the first group surgery had been performed only under coronary ischemia, whereas in the second group cardiac arrest was induced pharmacologically. IC CLUMP OCCURE MORE OFTEN. Also the amount of necessary defibrillation at occurrence of ventricular fibrillation was significantly lower. Besides it was extraordinary that after the cardiopulmonary bypass the consumption of positive inotropic drugs (Catecholamine) fsults injection-cardioplegia with its simple handling seemed evidently superior to coronary ischemia. But it remains to be seen, if injection-cardioplegia is equal to coronary perfusion.", "contents": "[Clinical experiences with cardioplegic injection in open heart surgery (author's transl)]. Two groups of 50 patients each who had undergone valve replacement with extracorporeal circulation, were examined. In the first group surgery had been performed only under coronary ischemia, whereas in the second group cardiac arrest was induced pharmacologically. IC CLUMP OCCURE MORE OFTEN. Also the amount of necessary defibrillation at occurrence of ventricular fibrillation was significantly lower. Besides it was extraordinary that after the cardiopulmonary bypass the consumption of positive inotropic drugs (Catecholamine) fsults injection-cardioplegia with its simple handling seemed evidently superior to coronary ischemia. But it remains to be seen, if injection-cardioplegia is equal to coronary perfusion."}
{"id": "PMID:1084600", "title": "[On myocardial abscess (author's transl)].", "content": "Authors describe an abscess in the area of a myocardial infarction. The possibility of surgical treatment is discussed.", "contents": "[On myocardial abscess (author's transl)]. Authors describe an abscess in the area of a myocardial infarction. The possibility of surgical treatment is discussed."}
{"id": "PMID:1084601", "title": "The influence of x-rays, vitamin A and protease inhibitor on the hydroproteolytic activity and serotonin content in pancreas and intestine of rats.", "content": "Activity of MAO, hydroproteolytic enzymes including some lysosomal markers as well as serotonin content were examined in pancreas and intestinal tissue of rats 24 hours after irradiation with the dose of 800R. It was stated that postirradiation disturbances of enzymatic activity in intestinal tract were accompanied by changes of serotonin content. Administration of vitamin A into rats caused in some parts of the intestine slight increase of acid phosphatase activity-and evident changes in serotonin content. Inhibitor of proteases (Trasylol) evidently prevented the disturbances of serotonin content both in rats exposed to X-rays or vitamin A administration- when simultaneously its influence on changes in hydroproteolytic activity (except of pancreas and colon) was of smaller degree.", "contents": "The influence of x-rays, vitamin A and protease inhibitor on the hydroproteolytic activity and serotonin content in pancreas and intestine of rats. Activity of MAO, hydroproteolytic enzymes including some lysosomal markers as well as serotonin content were examined in pancreas and intestinal tissue of rats 24 hours after irradiation with the dose of 800R. It was stated that postirradiation disturbances of enzymatic activity in intestinal tract were accompanied by changes of serotonin content. Administration of vitamin A into rats caused in some parts of the intestine slight increase of acid phosphatase activity-and evident changes in serotonin content. Inhibitor of proteases (Trasylol) evidently prevented the disturbances of serotonin content both in rats exposed to X-rays or vitamin A administration- when simultaneously its influence on changes in hydroproteolytic activity (except of pancreas and colon) was of smaller degree."}
{"id": "PMID:1084602", "title": "Pneumocystis and Histoplasma infections in wild animals from the Amazon region of Brazil.", "content": "Routine examination of tissues from wild forest rodents from Amap\u00e1, north Brazil, revealed Pneumocystis carinii in lung smears from a newly captured Oryzomys capito (Cricetidae). Acute, fatal infections with this parasite are also recorded in a number of captive \"coatimundis\", Nasua narica (Carnivora: Procyonidae) and a sloth, Bradypus tridactylus (Edentata). Pneumocystis was also encountered in lung smears from a newly captured and apparently healthy sloth, Choloepus didactylus. The presence of infection in newly captured animals leads us to believe that the fatal, fulminating pneumocystosis seen in the captive Nasua and Bradypus was due to exacerbation of pre-existant infections acquired in their natural forest environment. Pneumocystis carinii is a well known cause of fatal, interstitial plasma-cell pneumonia in human infants and sometimes the weakened adult: the keeping of exotic pets such as the coatimundi is, therefore, not without some hazard in this respect. Histoplasma, another well known pathogen for man, was isolated from 4 rodents, Proechimys guyanensis (Echimyidae), all from virgin forest along the newly opened Trans Amazon Highway, Par\u00e1 State, and from a single sloth, Choloepus didactylus, from near Bel\u00e9m, Par\u00e1. All these animals showed no symptoms of infection: isolation of the parasite was made by the inoculation of laboratory hamsters with saline suspensions of triturated liver and spleen.", "contents": "Pneumocystis and Histoplasma infections in wild animals from the Amazon region of Brazil. Routine examination of tissues from wild forest rodents from Amap\u00e1, north Brazil, revealed Pneumocystis carinii in lung smears from a newly captured Oryzomys capito (Cricetidae). Acute, fatal infections with this parasite are also recorded in a number of captive \"coatimundis\", Nasua narica (Carnivora: Procyonidae) and a sloth, Bradypus tridactylus (Edentata). Pneumocystis was also encountered in lung smears from a newly captured and apparently healthy sloth, Choloepus didactylus. The presence of infection in newly captured animals leads us to believe that the fatal, fulminating pneumocystosis seen in the captive Nasua and Bradypus was due to exacerbation of pre-existant infections acquired in their natural forest environment. Pneumocystis carinii is a well known cause of fatal, interstitial plasma-cell pneumonia in human infants and sometimes the weakened adult: the keeping of exotic pets such as the coatimundi is, therefore, not without some hazard in this respect. Histoplasma, another well known pathogen for man, was isolated from 4 rodents, Proechimys guyanensis (Echimyidae), all from virgin forest along the newly opened Trans Amazon Highway, Par\u00e1 State, and from a single sloth, Choloepus didactylus, from near Bel\u00e9m, Par\u00e1. All these animals showed no symptoms of infection: isolation of the parasite was made by the inoculation of laboratory hamsters with saline suspensions of triturated liver and spleen."}
{"id": "PMID:1084606", "title": "Cell-mediated immunity to H-2 antigens. Characteristics of the effector cells as detected in the microcytotoxicity assay.", "content": "Cell-mediated immune reactivity against murine histocompatibility antigens was compared by using both the microcytotoxicity assay (MCA) and the 51 chromium release assay (CRA). In both assay systems the data obtained showed T cell-mediated reactivity. In the CRA only T lymphocytes were detectable, which were cytotoxic, In contrast, in the MCA reactivity of nonlytic secondary T cells (NLST) was also detected. NLST acquired their MCA-reactivity within the first 24 hr of the 48-hr test period of the MCA. Circumstantial evidence is provided suggesting that MCA reactivity is a product of two different processes, namely, antigen-specific restimulation of immunt T cells and shedding of cytotoxicity inhibiting factors.", "contents": "Cell-mediated immunity to H-2 antigens. Characteristics of the effector cells as detected in the microcytotoxicity assay. Cell-mediated immune reactivity against murine histocompatibility antigens was compared by using both the microcytotoxicity assay (MCA) and the 51 chromium release assay (CRA). In both assay systems the data obtained showed T cell-mediated reactivity. In the CRA only T lymphocytes were detectable, which were cytotoxic, In contrast, in the MCA reactivity of nonlytic secondary T cells (NLST) was also detected. NLST acquired their MCA-reactivity within the first 24 hr of the 48-hr test period of the MCA. Circumstantial evidence is provided suggesting that MCA reactivity is a product of two different processes, namely, antigen-specific restimulation of immunt T cells and shedding of cytotoxicity inhibiting factors."}
{"id": "PMID:1084603", "title": "Purification and use of the C3d subunit C3.", "content": "A method is described for the preparation of C3d from fresh-frozen CPD plasma. A redissolved EDTA euglobulin precipitate formed from defibrinated plasma is chromatographed on DEAE cellulose. The C3-rich peak is further chromatographed by stepwise elution from hydroxy-apatite. The highly purified C3 and C3b so obtained are then treated with commercial C3b inactivator. G-200 sephadex filtration of this material produces a low molecular weight peak containing C3d greater than 95 per cent purity. The purified C3d, which contains negligible carbohydrate, has a molecular weight of 28,000 (based on SDS polyacrylamide gel electrophoresis). When added at a final concentration of less than 70 mug per ml, it completely inhibits the anti-C3d hemagglutinin reactivity of a potent anti-C3d antiglobulin serum but does not inhibit sera with reactivities against C3c, C4b, and C5b. Two of three rabbits hyperimmunized with purified C3d produced antisera exhibiting only anti-C3d precipitin activity. After absorption of heterologous antibodies, the antisera strongly agglutinated C3d-coated RBC, failed to agglutinate RBC glutinated C3d-coated RBC, failed to agglutinate RBC coated only with C4, and reacted weakly against strongly anti-Rh coated RBC. The latter reactivity was readily absorbed by whole IgG. Preliminary studies with 125I-labeled C3d indicate its potential value in assessing potency of anti-C3d reagents.", "contents": "Purification and use of the C3d subunit C3. A method is described for the preparation of C3d from fresh-frozen CPD plasma. A redissolved EDTA euglobulin precipitate formed from defibrinated plasma is chromatographed on DEAE cellulose. The C3-rich peak is further chromatographed by stepwise elution from hydroxy-apatite. The highly purified C3 and C3b so obtained are then treated with commercial C3b inactivator. G-200 sephadex filtration of this material produces a low molecular weight peak containing C3d greater than 95 per cent purity. The purified C3d, which contains negligible carbohydrate, has a molecular weight of 28,000 (based on SDS polyacrylamide gel electrophoresis). When added at a final concentration of less than 70 mug per ml, it completely inhibits the anti-C3d hemagglutinin reactivity of a potent anti-C3d antiglobulin serum but does not inhibit sera with reactivities against C3c, C4b, and C5b. Two of three rabbits hyperimmunized with purified C3d produced antisera exhibiting only anti-C3d precipitin activity. After absorption of heterologous antibodies, the antisera strongly agglutinated C3d-coated RBC, failed to agglutinate RBC glutinated C3d-coated RBC, failed to agglutinate RBC coated only with C4, and reacted weakly against strongly anti-Rh coated RBC. The latter reactivity was readily absorbed by whole IgG. Preliminary studies with 125I-labeled C3d indicate its potential value in assessing potency of anti-C3d reagents."}
{"id": "PMID:1084604", "title": "Autologous blood transfusion in coronary artery bypass surgery.", "content": "Forty-four patients undergoing coronary artery bypass surgery participated in an autologous blood donor program. Blood was collected at weekly intervals up to three days prior to the scheduled date of surgery. Sixteen patients donated a total of two autologous units each, and 28 patients donated one autologous unit each. No donor morbidity or mortality was encountered during or following blood donation. Autologous blood accounted for more than one-third of all blood required by these patients throughout their hospital courses. Autologous transfusion appears to be a safe procedure for patients with severe coronary artery disease.", "contents": "Autologous blood transfusion in coronary artery bypass surgery. Forty-four patients undergoing coronary artery bypass surgery participated in an autologous blood donor program. Blood was collected at weekly intervals up to three days prior to the scheduled date of surgery. Sixteen patients donated a total of two autologous units each, and 28 patients donated one autologous unit each. No donor morbidity or mortality was encountered during or following blood donation. Autologous blood accounted for more than one-third of all blood required by these patients throughout their hospital courses. Autologous transfusion appears to be a safe procedure for patients with severe coronary artery disease."}
{"id": "PMID:1084608", "title": "Functional characterization of a naturally occurring antibody cytotoxic for a subpopulation of splenic T cells.", "content": "Sera from old (greater than 6 months) New Zealand Black (NZB) mice were shown to contain an IgM antibody which was cytotoxic for thymus-derived (T) lymphocytes in the presence of rabbit complement. This antibody in the presence of complement markedly decreased the response of normal spleen cells to the T cell mitogen concanvalin A (Con A) but not to another T cell mitogen phytohemagglutinin-P (PHA-P). Responses of these treated cells to the polyclonal B cell mitogens, bacterial lipopolysaccharide (LPS), and polyriboinosinic-polyribocytidylic acid (poly[I]-poly[C]) were unimpaired. This naturally occurring NZB antibody was different from conventional anti-theta serum which abolished the response to both PHA-P and Con A. Moreover, the NZB serum affected spleen cells from both theta-C3H and theta-AKR mice. The ability of such serum to influence, differentially, Con A reactive cells as opposed to PHA-P-reactive cells, suggest that the NZB antiserum recognizes a subpopulation of T lymphocytes.", "contents": "Functional characterization of a naturally occurring antibody cytotoxic for a subpopulation of splenic T cells. Sera from old (greater than 6 months) New Zealand Black (NZB) mice were shown to contain an IgM antibody which was cytotoxic for thymus-derived (T) lymphocytes in the presence of rabbit complement. This antibody in the presence of complement markedly decreased the response of normal spleen cells to the T cell mitogen concanvalin A (Con A) but not to another T cell mitogen phytohemagglutinin-P (PHA-P). Responses of these treated cells to the polyclonal B cell mitogens, bacterial lipopolysaccharide (LPS), and polyriboinosinic-polyribocytidylic acid (poly[I]-poly[C]) were unimpaired. This naturally occurring NZB antibody was different from conventional anti-theta serum which abolished the response to both PHA-P and Con A. Moreover, the NZB serum affected spleen cells from both theta-C3H and theta-AKR mice. The ability of such serum to influence, differentially, Con A reactive cells as opposed to PHA-P-reactive cells, suggest that the NZB antiserum recognizes a subpopulation of T lymphocytes."}
{"id": "PMID:1084611", "title": "Localization of intracular and intraorbital foreign bodies by means of A-scan ultrasonography.", "content": "This paper discusses the use of ultrasonography in ophthalmology for foreign body localization and detection. The problems and advantages of using A-scann methods to verify and improve upon x-ray diagnosis are considered.", "contents": "Localization of intracular and intraorbital foreign bodies by means of A-scan ultrasonography. This paper discusses the use of ultrasonography in ophthalmology for foreign body localization and detection. The problems and advantages of using A-scann methods to verify and improve upon x-ray diagnosis are considered."}
{"id": "PMID:1084614", "title": "The impulsive test in man.", "content": "The impulsive test is brief and comfortable to the patient. The SVM is the best discriminator of the variables examined. By using percent differences between CW and CCW responses in the same subject at the same stimulus magnitude, we can define a range for the normal population and indicate patients outside that normal range. The impulsive test gives directional information in patients with unilateral lesions. In patients with severe bilateral loss of vestibular function, measurable responses are still obtainable; while in patients with primary cerebellar disease, responses are increased. The test may also be useful in giving anatomic information about posterior fossa lesions.", "contents": "The impulsive test in man. The impulsive test is brief and comfortable to the patient. The SVM is the best discriminator of the variables examined. By using percent differences between CW and CCW responses in the same subject at the same stimulus magnitude, we can define a range for the normal population and indicate patients outside that normal range. The impulsive test gives directional information in patients with unilateral lesions. In patients with severe bilateral loss of vestibular function, measurable responses are still obtainable; while in patients with primary cerebellar disease, responses are increased. The test may also be useful in giving anatomic information about posterior fossa lesions."}
{"id": "PMID:1084624", "title": "T cell deficiency in patients with autoimmune hemolytic anemia ('warm type').", "content": "19 patients with chronic 'warm type' autoimmune hemolytic anemia were studied for abnormalities of cellular immune reactions. Evidence was obtained for a reduction of rosette-forming cells (RFC). Lymphocytotoxic antibodies were present in only 8 patients and correlated, with only one exception, with a reduced number of RFC. No significant deviation from normal ranges of the three major immunoglobulin classes in the patients' sera were found. C3 and C4 complement components were also, with one exception, within normal limits. In 18 of 19 patients no apparent association existed between the type or the amount of autoantibodies and/or complement components fixed on red cells and the levels of the respective immunoglobulins or complement in the sera.", "contents": "T cell deficiency in patients with autoimmune hemolytic anemia ('warm type'). 19 patients with chronic 'warm type' autoimmune hemolytic anemia were studied for abnormalities of cellular immune reactions. Evidence was obtained for a reduction of rosette-forming cells (RFC). Lymphocytotoxic antibodies were present in only 8 patients and correlated, with only one exception, with a reduced number of RFC. No significant deviation from normal ranges of the three major immunoglobulin classes in the patients' sera were found. C3 and C4 complement components were also, with one exception, within normal limits. In 18 of 19 patients no apparent association existed between the type or the amount of autoantibodies and/or complement components fixed on red cells and the levels of the respective immunoglobulins or complement in the sera."}
{"id": "PMID:1084627", "title": "[The diagnosis of subtentorial tumours by vertebral angiography (author's transl)].", "content": "The diagnosis of space-occupying lesions in the subtentorial space by vertebral angiography is based upon a good knowledge of arteries and veins and topographic points of this region. An imaginary line between the colliculo-central point and the chorioidal point divides the posterior fossa into two imaginary parts. In this way it is possible to localize displacements in the region of the posterior fossa. Of course, the displacement of the veins and arteries is very important in the verification of the tumours. Consequently, vertebral angiography is of vital importance in the investigation of space-occupying lesions in the posterior fossa.", "contents": "[The diagnosis of subtentorial tumours by vertebral angiography (author's transl)]. The diagnosis of space-occupying lesions in the subtentorial space by vertebral angiography is based upon a good knowledge of arteries and veins and topographic points of this region. An imaginary line between the colliculo-central point and the chorioidal point divides the posterior fossa into two imaginary parts. In this way it is possible to localize displacements in the region of the posterior fossa. Of course, the displacement of the veins and arteries is very important in the verification of the tumours. Consequently, vertebral angiography is of vital importance in the investigation of space-occupying lesions in the posterior fossa."}
{"id": "PMID:1084630", "title": "[Function improvement in levography following aortocoronary bypass].", "content": "Actively contracting segments, preoperatively akinetic, were found in 8 of 63 patients, evaluated 6-12 months after aortocoronary surgery by coronary angiography. Ejection fraction was increased from 48.1% (S.D. 15.7) to 68.3% (S.D. 11.4). These patients are characterized by two simple clinical parameters: 1.  All patients had angina pectoris at rest or at minimum exercise except for one; 2. preoperatively, there was a discrepancy between severe ventriculographic and discreet Ecg findings. These findings prove that myocardial function in coronary artery disease can be impaired at rest by ischemia, without clinical signs of coronary insufficiency, such as angina pectoris. Even severe impairment of left ventricular function is no contraindication for coronary artery surgery, if caused by reversible myocardial ischemia.", "contents": "[Function improvement in levography following aortocoronary bypass]. Actively contracting segments, preoperatively akinetic, were found in 8 of 63 patients, evaluated 6-12 months after aortocoronary surgery by coronary angiography. Ejection fraction was increased from 48.1% (S.D. 15.7) to 68.3% (S.D. 11.4). These patients are characterized by two simple clinical parameters: 1.  All patients had angina pectoris at rest or at minimum exercise except for one; 2. preoperatively, there was a discrepancy between severe ventriculographic and discreet Ecg findings. These findings prove that myocardial function in coronary artery disease can be impaired at rest by ischemia, without clinical signs of coronary insufficiency, such as angina pectoris. Even severe impairment of left ventricular function is no contraindication for coronary artery surgery, if caused by reversible myocardial ischemia."}
{"id": "PMID:1084631", "title": "Ciliated glial cells and neurons in the interpeduncular nucleus of the frog.", "content": "Some neurons and glial cells of the interpeduncular nucleus of the adult frog Rana esculenta contain one cilium. The cilium complex shows two-centriole basal organization. Cross striated structures are seen apposed to the centriolar formations. The centriole and associated basal body are always close to the vesicular elements of the Golgi apparatus in the neurons and are surrounded by quite a number of microtubules in the glial cells. The observations suggest that these ciliated cells represent specialized elements in which the cilium has probably the same functional transport properties postulated for cytoplasmic microtubules.", "contents": "Ciliated glial cells and neurons in the interpeduncular nucleus of the frog. Some neurons and glial cells of the interpeduncular nucleus of the adult frog Rana esculenta contain one cilium. The cilium complex shows two-centriole basal organization. Cross striated structures are seen apposed to the centriolar formations. The centriole and associated basal body are always close to the vesicular elements of the Golgi apparatus in the neurons and are surrounded by quite a number of microtubules in the glial cells. The observations suggest that these ciliated cells represent specialized elements in which the cilium has probably the same functional transport properties postulated for cytoplasmic microtubules."}
{"id": "PMID:1084633", "title": "Aorto-coronary graft for dissected coronary artery.", "content": "A 46-year-old woman while undergoing selective coronary cine angiogram dissected her coronary artery. She was managed by heparin therapy followed by urgent aorto-coronary artery saphenous vein graft with no consequences.", "contents": "Aorto-coronary graft for dissected coronary artery. A 46-year-old woman while undergoing selective coronary cine angiogram dissected her coronary artery. She was managed by heparin therapy followed by urgent aorto-coronary artery saphenous vein graft with no consequences."}
{"id": "PMID:1084634", "title": "Valve replacement and coronary revascularization: combined surgical approach.", "content": "Report is presented of sixteen patients who had coronary bypass procedures performed in combination with valvular surgery. There was a hospital mortality of two patients of 12.5%. Significant improvement occurred in the remaining fourteen patients or 87.5%. Of these fourteen patients, one patient required re-operation for mitral valve replacement following an open mitral commissurotomy two years previously. Coronary arteriosclerosis complicating the outcome of patients undergoing valvular surgery is well recognized (1). Hall and associates (2) reported the results of seventy-two patients undergoing combined procedures with seventeen operative deaths, an operative mortality rate of 23.6%. Merin's group (3) reported on a series of fifty patients from the Mayo Clinic with an operative mortality rate of 14%. Loop and coworkers (4) reported an operative mortality rate of 8% in fifty patients undergoing combined procedures. Crouch and associates (5) reported on thirty-six patients with four hospital deaths, an operative mortality of 11%. At the Heart Institute of Michigan, five hundred fifty-six patients under-went coronary bypass surgery from December, 1969 to September, 1974. This is an all inclusive, consecutive experience. Overall mortality in this group was nine patients or 1.6%. Of five hundred forty patients who did not have associated valvular surgery, the mortality was seven patients or 1.3%. The purpose of this presentation is to elaborate on the sixteen patients who had coronary surgery combined with valvular surgery. Mortality in this group was two patients or 12.5%.", "contents": "Valve replacement and coronary revascularization: combined surgical approach. Report is presented of sixteen patients who had coronary bypass procedures performed in combination with valvular surgery. There was a hospital mortality of two patients of 12.5%. Significant improvement occurred in the remaining fourteen patients or 87.5%. Of these fourteen patients, one patient required re-operation for mitral valve replacement following an open mitral commissurotomy two years previously. Coronary arteriosclerosis complicating the outcome of patients undergoing valvular surgery is well recognized (1). Hall and associates (2) reported the results of seventy-two patients undergoing combined procedures with seventeen operative deaths, an operative mortality rate of 23.6%. Merin's group (3) reported on a series of fifty patients from the Mayo Clinic with an operative mortality rate of 14%. Loop and coworkers (4) reported an operative mortality rate of 8% in fifty patients undergoing combined procedures. Crouch and associates (5) reported on thirty-six patients with four hospital deaths, an operative mortality of 11%. At the Heart Institute of Michigan, five hundred fifty-six patients under-went coronary bypass surgery from December, 1969 to September, 1974. This is an all inclusive, consecutive experience. Overall mortality in this group was nine patients or 1.6%. Of five hundred forty patients who did not have associated valvular surgery, the mortality was seven patients or 1.3%. The purpose of this presentation is to elaborate on the sixteen patients who had coronary surgery combined with valvular surgery. Mortality in this group was two patients or 12.5%."}
{"id": "PMID:1084654", "title": "[The relationship between the clinical picture and course of acoustic nerve tumors and the direction of their growth in the cerebellopontine angle].", "content": "The data of clinical, surgical and post-mortem studies in 95 patients with neurinomas of the acoustic nerve permitted to eliminate 3 variants in the growth of neoplastical process into the ponto-cerebellar angle: 1) subtentorial (the upper variant) -- 27 cases; 2) the middle line of the brain stem (middle variant) -- 15 cases; 3) the lower parts of the angle (lower variant)--52 cases. Depending upon the direction of the growth into this space and upon the stage of the disease there were differences in the succession, frequency and expressiveness of focal and general brain symptoms. The development of the disease also differed.", "contents": "[The relationship between the clinical picture and course of acoustic nerve tumors and the direction of their growth in the cerebellopontine angle]. The data of clinical, surgical and post-mortem studies in 95 patients with neurinomas of the acoustic nerve permitted to eliminate 3 variants in the growth of neoplastical process into the ponto-cerebellar angle: 1) subtentorial (the upper variant) -- 27 cases; 2) the middle line of the brain stem (middle variant) -- 15 cases; 3) the lower parts of the angle (lower variant)--52 cases. Depending upon the direction of the growth into this space and upon the stage of the disease there were differences in the succession, frequency and expressiveness of focal and general brain symptoms. The development of the disease also differed."}
{"id": "PMID:1084655", "title": "[Rhythm disorders occurring after surgical closure of the interatrial communication].", "content": "A detailed evaluation is presented about the frequency and the type of arrhythmias following the surgical correction of 147 cases of atrial septal defects not associated with other congenital heart malformations. A total of 131 arrhythmias occurred in 84 patients. The nodal and the coronary sinus rhythms are the most frequently encountered after surgery, attaining 53,8% of all arrhythmias, while atrial fibrillation or flutter represented about 22%. The follow-up of these arrhythmias concerned the 3 month period immediately after surgery. After that time 20% of the arrhythmias occurring in the early postoperative period persisted. The frequency of arrhythmias increased with age, was relatively independent of the importance of the left to right shunt, the level of the mean pulmonary artery pressure and the sex of the patients; but statistically increased in patients in whom the correction was made with a patch. The authors concluded that there must exist at least two mechanisms to explain the occurrence of arrhythmias. A first one must be in relation to the surgical manipulation; the second, which is only an hypothesis, stipulates an alteration of the conduction pathways due to the auricular distention or the existence of a congenital anomaly of the conduction pathways.", "contents": "[Rhythm disorders occurring after surgical closure of the interatrial communication]. A detailed evaluation is presented about the frequency and the type of arrhythmias following the surgical correction of 147 cases of atrial septal defects not associated with other congenital heart malformations. A total of 131 arrhythmias occurred in 84 patients. The nodal and the coronary sinus rhythms are the most frequently encountered after surgery, attaining 53,8% of all arrhythmias, while atrial fibrillation or flutter represented about 22%. The follow-up of these arrhythmias concerned the 3 month period immediately after surgery. After that time 20% of the arrhythmias occurring in the early postoperative period persisted. The frequency of arrhythmias increased with age, was relatively independent of the importance of the left to right shunt, the level of the mean pulmonary artery pressure and the sex of the patients; but statistically increased in patients in whom the correction was made with a patch. The authors concluded that there must exist at least two mechanisms to explain the occurrence of arrhythmias. A first one must be in relation to the surgical manipulation; the second, which is only an hypothesis, stipulates an alteration of the conduction pathways due to the auricular distention or the existence of a congenital anomaly of the conduction pathways."}
{"id": "PMID:1084656", "title": "Can pulmonary artery pressure be predicted without right heart catheterization in chronic obstructive lung disease?", "content": "We have investigated the dependance of observed pulmonary artery mean pressure (PAP) on body surface, age, PaO2, pH, PaCO2, hematocrit, and spirometric data (VC, FEV1/VC, RV/TLC) in 70 patients with chronic obstructive lung disease (COLD). After elimination of all variables that failed to correlate with PAP, multifactorial analysis showed that only two of nine independent variables, namely PaO2 and body surface, correlated significantly with PAP. According to our calculations, 28.9% of total PAP is predictable by PaO2, 1.5% by H+ concentration, 2.8% by RV/TLC, and 2.5% by body surface. Fully 64.2% of total variability was not accounted for by our regression analysis; thus the error of predicted PAP was so great (+/- 17 mm Hg for P = 0.05) as to invalidate the method. We also recalculated our subjects' PAP values by applying Enson's and Grassi's equations to our own lung function and biochemical data, and compared the predicted PAP values thus obtained with those measured directly in our subjects. Both equations proved imprecise and/or inaccurate in the individual case. From this we conclude that whereas available equations may be suitable for predicting the mean PAP value of a large population sample, the same equations cannot give a reliable prediction in individual cases.", "contents": "Can pulmonary artery pressure be predicted without right heart catheterization in chronic obstructive lung disease? We have investigated the dependance of observed pulmonary artery mean pressure (PAP) on body surface, age, PaO2, pH, PaCO2, hematocrit, and spirometric data (VC, FEV1/VC, RV/TLC) in 70 patients with chronic obstructive lung disease (COLD). After elimination of all variables that failed to correlate with PAP, multifactorial analysis showed that only two of nine independent variables, namely PaO2 and body surface, correlated significantly with PAP. According to our calculations, 28.9% of total PAP is predictable by PaO2, 1.5% by H+ concentration, 2.8% by RV/TLC, and 2.5% by body surface. Fully 64.2% of total variability was not accounted for by our regression analysis; thus the error of predicted PAP was so great (+/- 17 mm Hg for P = 0.05) as to invalidate the method. We also recalculated our subjects' PAP values by applying Enson's and Grassi's equations to our own lung function and biochemical data, and compared the predicted PAP values thus obtained with those measured directly in our subjects. Both equations proved imprecise and/or inaccurate in the individual case. From this we conclude that whereas available equations may be suitable for predicting the mean PAP value of a large population sample, the same equations cannot give a reliable prediction in individual cases."}
{"id": "PMID:1084657", "title": "[Clinical experimentation with intravenously administered disopyramide].", "content": "The intravenous injection of disopyramide (1.5 mg/kg) induces the return to sinus rhythm in about 60% of arrhythmias. When there exist perturbations of the cardiac rhythm, secondary to recent myocardial infarction, the percentage of success reaches 70%. The drug induces the disappearance of the extrasystoles in 80% of the cases, whatever the nature of the underlying cardiopathy. Although the secondary effects of the drug are slight, it is advisable to administer the substance in a slow intravenous injection (5 minutes), while controlling the arterial pressure and the E.C.G.", "contents": "[Clinical experimentation with intravenously administered disopyramide]. The intravenous injection of disopyramide (1.5 mg/kg) induces the return to sinus rhythm in about 60% of arrhythmias. When there exist perturbations of the cardiac rhythm, secondary to recent myocardial infarction, the percentage of success reaches 70%. The drug induces the disappearance of the extrasystoles in 80% of the cases, whatever the nature of the underlying cardiopathy. Although the secondary effects of the drug are slight, it is advisable to administer the substance in a slow intravenous injection (5 minutes), while controlling the arterial pressure and the E.C.G."}
{"id": "PMID:1084658", "title": "The prognostic implications of systolic time intervals after acute myocardial infarction.", "content": "STI were measured on the 7th day after acute myocardial infarction in 144 male patients. These patients were reviewed at 6 weeks and 86 were reinvestigated and reviewed at 6 months. The STI could not predict the patients who would have either angina or a further myocardial infarction. Left ventricular function, as measured by STI, appeared to be a minor factor governing return to work after myocardial infarction. Patients who had developed ventricular aneurysms at 6 months had significantly increased EMI at 6 weeks. STI on day 7 had considerable prognostic significance for left ventricular failure at 6 weeks and death between day 7 and 6 weeks and it is suggested that LVET index could be usefully, and easily, introduced as a parameter after myocardial infarction.", "contents": "The prognostic implications of systolic time intervals after acute myocardial infarction. STI were measured on the 7th day after acute myocardial infarction in 144 male patients. These patients were reviewed at 6 weeks and 86 were reinvestigated and reviewed at 6 months. The STI could not predict the patients who would have either angina or a further myocardial infarction. Left ventricular function, as measured by STI, appeared to be a minor factor governing return to work after myocardial infarction. Patients who had developed ventricular aneurysms at 6 months had significantly increased EMI at 6 weeks. STI on day 7 had considerable prognostic significance for left ventricular failure at 6 weeks and death between day 7 and 6 weeks and it is suggested that LVET index could be usefully, and easily, introduced as a parameter after myocardial infarction."}
{"id": "PMID:1084659", "title": "Clinical and circulatory effects of oral isosorbide dinitrate in patients with angina during exercise.", "content": "Isosorbide dinitrate was given orally to 6 patients with anginal pain on exertion and its effect on exercise capacity, ECG and other hemodynamic parameters was compared with the control values and with the results of a group of 6 patients who received a placebo. The drug seems to affect favourably the working capacity and the ECG on exercise for a longer period than NTG while we do not observe a placebo effect.", "contents": "Clinical and circulatory effects of oral isosorbide dinitrate in patients with angina during exercise. Isosorbide dinitrate was given orally to 6 patients with anginal pain on exertion and its effect on exercise capacity, ECG and other hemodynamic parameters was compared with the control values and with the results of a group of 6 patients who received a placebo. The drug seems to affect favourably the working capacity and the ECG on exercise for a longer period than NTG while we do not observe a placebo effect."}
{"id": "PMID:1084660", "title": "Persistent atrial standstill.", "content": "The authors present a case of permanent atrial standstill with syncopal attacks, in a patient with chronic Chagas' Heart Disease. The recognition of this dysrhythmia was based upon the conventional and intracavity electrocardiographic tracings in addition to phonomecanographic and hemodynamic data. The recording of the His Bundle electrogram demonstrated the absence of atrial activity, with the His potential preceding all ventricular complexes and an advanced conduction defect distal to the bundle of His. A diffuse type of atrial involvement was suggested by the lack of response to pacemaker stimulation. An increase in ventricular rate following intravenous atropine administration, led to the diagnosis of an a-v junctional rhythm with a widened QRS complex due to an associated right bundle branch block. Following the implantation of an epicardial ventricular pacemaker, the patient became completely asymptomatic despite the persistence of electrical and mechanical atrial standstill.", "contents": "Persistent atrial standstill. The authors present a case of permanent atrial standstill with syncopal attacks, in a patient with chronic Chagas' Heart Disease. The recognition of this dysrhythmia was based upon the conventional and intracavity electrocardiographic tracings in addition to phonomecanographic and hemodynamic data. The recording of the His Bundle electrogram demonstrated the absence of atrial activity, with the His potential preceding all ventricular complexes and an advanced conduction defect distal to the bundle of His. A diffuse type of atrial involvement was suggested by the lack of response to pacemaker stimulation. An increase in ventricular rate following intravenous atropine administration, led to the diagnosis of an a-v junctional rhythm with a widened QRS complex due to an associated right bundle branch block. Following the implantation of an epicardial ventricular pacemaker, the patient became completely asymptomatic despite the persistence of electrical and mechanical atrial standstill."}
{"id": "PMID:1084661", "title": "Double-inlet left ventricle with absent sixth aortic arch and multiple cardiac malformations.", "content": "The first well-documented case of double-inlet left ventricle in association with absence of the derivatives of the sixth aortic arch of foetal life (the right and left branches of the pulmonary artery and the ductus arteriosus), is reported. In addition there were a total anomalous pulmonary venous connection to the portal vein, an ostium secundum type atrial septal defect and dextrocardia.", "contents": "Double-inlet left ventricle with absent sixth aortic arch and multiple cardiac malformations. The first well-documented case of double-inlet left ventricle in association with absence of the derivatives of the sixth aortic arch of foetal life (the right and left branches of the pulmonary artery and the ductus arteriosus), is reported. In addition there were a total anomalous pulmonary venous connection to the portal vein, an ostium secundum type atrial septal defect and dextrocardia."}
{"id": "PMID:1084662", "title": "[Diagnostic significance of phono-mechanographic medication of constrictive endocardial fibrosis (or endomyocardial fibrosis)].", "content": "The phonomecanographic investigation of 12 patients with FEC allow us to recognize diagnostics signs of great interest. 1. The ventricular aspect of the jugular veinous pulse involving a positive systolic wave and a diastolic aspect in \"dip-plateau\" is a chief indication of a right ventricular fibrosis. This indication is more significant of FEC when the nadir of the dip is far above the base line, when the dip-rising time is short, when the telediastolic ratio is high (more important than 0.40) when the electro-mechanical latency time is prolonged and when the isovolumetric relaxation time is normal. 2. The apexogram aspect in diastolic dip-plateau is a capital indication for a fibrosis of the left ventricule. This indication is more significant when the nadir of the dip is above the baseline, when the telediastolic ratio is high (more important than 0.30) when the electromechanical latency time and the isovolumetric relaxation time are normal. 3. The protodiastolic endocardial vibrancy recorded during the dip climbing and showing the features we described, seems to us to be a very important sign. This vibrancy can be preceded or/and followed by a short and early diastolic murmur of low frequency, always finishing at the end of the dip. 4. Recording of pulmonary valves or atrio-ventricular murmur is an important diagnostic feature in an adiastolic syndrome. 5. Recording of a pulmoanry infundibular impulse is an indication for a right ventricular fibrosis. This indication is very significant if the tracing is a dip-plateau.", "contents": "[Diagnostic significance of phono-mechanographic medication of constrictive endocardial fibrosis (or endomyocardial fibrosis)]. The phonomecanographic investigation of 12 patients with FEC allow us to recognize diagnostics signs of great interest. 1. The ventricular aspect of the jugular veinous pulse involving a positive systolic wave and a diastolic aspect in \"dip-plateau\" is a chief indication of a right ventricular fibrosis. This indication is more significant of FEC when the nadir of the dip is far above the base line, when the dip-rising time is short, when the telediastolic ratio is high (more important than 0.40) when the electro-mechanical latency time is prolonged and when the isovolumetric relaxation time is normal. 2. The apexogram aspect in diastolic dip-plateau is a capital indication for a fibrosis of the left ventricule. This indication is more significant when the nadir of the dip is above the baseline, when the telediastolic ratio is high (more important than 0.30) when the electromechanical latency time and the isovolumetric relaxation time are normal. 3. The protodiastolic endocardial vibrancy recorded during the dip climbing and showing the features we described, seems to us to be a very important sign. This vibrancy can be preceded or/and followed by a short and early diastolic murmur of low frequency, always finishing at the end of the dip. 4. Recording of pulmonary valves or atrio-ventricular murmur is an important diagnostic feature in an adiastolic syndrome. 5. Recording of a pulmoanry infundibular impulse is an indication for a right ventricular fibrosis. This indication is very significant if the tracing is a dip-plateau."}
{"id": "PMID:1084663", "title": "Thrombotic obstruction of Bjork-Shiley tricuspid valve prosthesis. Report of three cases reoperated with success.", "content": "Three patients presented subacute right heart failure, 6, 12 and 22 months after replacement of the tricuspid valve with a Bj\u00f6rk-Shiley prosthesis. The principal finding encountered on clinical examination was a tricuspid filling murmur with inspiratory accentuation. The mean prosthetic gradient found at cardiac catheterization was 7, 8 and 10 mm Hg. At surgery, the three prostheses were thrombosed on their ventricular aspect and all the three were replaced with new ones of the same model. The patients are alive and in good condition, 21, 17 and 8 months after the operation. Problems which relate to the surgical management of tricuspid valve disease are discussed.", "contents": "Thrombotic obstruction of Bjork-Shiley tricuspid valve prosthesis. Report of three cases reoperated with success. Three patients presented subacute right heart failure, 6, 12 and 22 months after replacement of the tricuspid valve with a Bj\u00f6rk-Shiley prosthesis. The principal finding encountered on clinical examination was a tricuspid filling murmur with inspiratory accentuation. The mean prosthetic gradient found at cardiac catheterization was 7, 8 and 10 mm Hg. At surgery, the three prostheses were thrombosed on their ventricular aspect and all the three were replaced with new ones of the same model. The patients are alive and in good condition, 21, 17 and 8 months after the operation. Problems which relate to the surgical management of tricuspid valve disease are discussed."}
{"id": "PMID:1084664", "title": "[Epidemiology of the coronary risk in a semi-rural population of 420 patients having presented a myocardial infarct].", "content": "Various sociological and biological parameters have been studied in a population of 420 subjects - 335 men and 85 women - living in a semi rural country and having presented a myocardial infarct. Our study shows big differences in epidemiology between men and women: 1. The age of women is on an average seven years older than men (63 years against 56). 2. The women's average rate of cholesterol oversteps men's rate by 21 mg% (291 mg % against 271). 3. The blood pressure, systolic and diastolic, is distinctly higher by women (mean 171/97) tan by men (148/98). Amid women, 63% have a S.B.P. equal to or higher than 160 mm Hg against 23% by men and 56% have a D.B.P. equal to or higher than 100 mm Hg against 21 % by men. 4. Hyperglycemia is more frequent among women; man rate: 103 mg % against 88 mg % by men; 17 % of the women have an equal to or higher rate than 110 mg % (8 % among men). 5. Obesity, expressed by a weight index, equal to or higher than 120, is found in 76 % of the women and 34 % of the men. The mean index is 123 by the women and 115 by the men. 6. Cigarette smoking is far more important by men: 65 % of the men smoke at least 15 cigarettes a day with smoke inhalation. This factor is practically not met by women. The smokers get their myocardial infarct at a significantly lower age than no smoking men and women. The women of our infarct population have thus an age, a cholesterol rate, a blood pressure and a glycemia higher than men of the same population; they are more frequently overweight but they don't smoke. Consequently, metabolic agents seem to play an essential part in coronary risk in women.", "contents": "[Epidemiology of the coronary risk in a semi-rural population of 420 patients having presented a myocardial infarct]. Various sociological and biological parameters have been studied in a population of 420 subjects - 335 men and 85 women - living in a semi rural country and having presented a myocardial infarct. Our study shows big differences in epidemiology between men and women: 1. The age of women is on an average seven years older than men (63 years against 56). 2. The women's average rate of cholesterol oversteps men's rate by 21 mg% (291 mg % against 271). 3. The blood pressure, systolic and diastolic, is distinctly higher by women (mean 171/97) tan by men (148/98). Amid women, 63% have a S.B.P. equal to or higher than 160 mm Hg against 23% by men and 56% have a D.B.P. equal to or higher than 100 mm Hg against 21 % by men. 4. Hyperglycemia is more frequent among women; man rate: 103 mg % against 88 mg % by men; 17 % of the women have an equal to or higher rate than 110 mg % (8 % among men). 5. Obesity, expressed by a weight index, equal to or higher than 120, is found in 76 % of the women and 34 % of the men. The mean index is 123 by the women and 115 by the men. 6. Cigarette smoking is far more important by men: 65 % of the men smoke at least 15 cigarettes a day with smoke inhalation. This factor is practically not met by women. The smokers get their myocardial infarct at a significantly lower age than no smoking men and women. The women of our infarct population have thus an age, a cholesterol rate, a blood pressure and a glycemia higher than men of the same population; they are more frequently overweight but they don't smoke. Consequently, metabolic agents seem to play an essential part in coronary risk in women."}
{"id": "PMID:1084665", "title": "[Long-term prognosis of myocardial infarct].", "content": "The authors study the long-term prognosis of a population of male subjects having survived 24 to 48 hours to their first myocardial infarction. The mean annual mortality is 6%. The long-term cumulated survival is particualarly influenced by a high blood pressure and by heart failure occurring during the acute episode and in a lesser proportion by age; the prognosis at long-term is not or little influenced by family history, cholesterolemia, cigarette smoking or the presence of angina before infarction. The presence or absence of heart failure and high blood pressure allows to make sub-groups with very different long-term prognosis. In the framework of secondary prevention of ischaemic heart diseases, the authors propose to start a controlled study implying both a programme of physical activity and a long-term energical treatment of arterial hypertension.", "contents": "[Long-term prognosis of myocardial infarct]. The authors study the long-term prognosis of a population of male subjects having survived 24 to 48 hours to their first myocardial infarction. The mean annual mortality is 6%. The long-term cumulated survival is particualarly influenced by a high blood pressure and by heart failure occurring during the acute episode and in a lesser proportion by age; the prognosis at long-term is not or little influenced by family history, cholesterolemia, cigarette smoking or the presence of angina before infarction. The presence or absence of heart failure and high blood pressure allows to make sub-groups with very different long-term prognosis. In the framework of secondary prevention of ischaemic heart diseases, the authors propose to start a controlled study implying both a programme of physical activity and a long-term energical treatment of arterial hypertension."}
{"id": "PMID:1084666", "title": "Refractory cardiac arrhythmia due to hypomagnesmia.", "content": "A case of ventricular bigeminy possibly due to magnesium deficiency is reported. Magnesium sulphate was successfully used for its management. Differential diagnosis of idiopathic cardiac arrhythmias should always include hypomagnesemia as one of the causes and magnesium should be replaced for its proper management.", "contents": "Refractory cardiac arrhythmia due to hypomagnesmia. A case of ventricular bigeminy possibly due to magnesium deficiency is reported. Magnesium sulphate was successfully used for its management. Differential diagnosis of idiopathic cardiac arrhythmias should always include hypomagnesemia as one of the causes and magnesium should be replaced for its proper management."}
{"id": "PMID:1084667", "title": "[Epidemiology of cardiovascular disease in the urban population of the town of Pahlavi (North Iran)].", "content": "A survey of cardiovascular diseases was carried out in June-August 1973 in the city of Pahlavi, a port in the Caspian area in Iran. A representative sample was taken from persons aged 35 years and older from both sexes. The prevalence of high blood pressure (WHO definition) is 29,7% in men and 31,0% in women of this age group. ECG tracings were prepared and the prevalence of various abnormalities are presented. A considerable proportion of the population have ST segment and T wave abnormalities. Correlation between ECG abnormalities and blood pressure figures has been studied. This study shows that there is a relationship between ECG abnormalities of group 3, 4 and 5 (Minnesota Coding) and the levels of blood pressure.", "contents": "[Epidemiology of cardiovascular disease in the urban population of the town of Pahlavi (North Iran)]. A survey of cardiovascular diseases was carried out in June-August 1973 in the city of Pahlavi, a port in the Caspian area in Iran. A representative sample was taken from persons aged 35 years and older from both sexes. The prevalence of high blood pressure (WHO definition) is 29,7% in men and 31,0% in women of this age group. ECG tracings were prepared and the prevalence of various abnormalities are presented. A considerable proportion of the population have ST segment and T wave abnormalities. Correlation between ECG abnormalities and blood pressure figures has been studied. This study shows that there is a relationship between ECG abnormalities of group 3, 4 and 5 (Minnesota Coding) and the levels of blood pressure."}
{"id": "PMID:1084668", "title": "Postventriculotomy aneurysm and left ventricular dysfunction following closed mitral commissurotomy.", "content": "A ventricular aneurysm at the site of a previous transventricular commissurotomy for mitral stenosis was diagnosed in a 39 year old female. Catheterization data showed abnormal left ventricular response to exercise and to increased afterload. Death occurred following mitral valve replacement. At autopsy no cause other than left ventricular aneurysm could be established to explain left ventricular dysfunction.", "contents": "Postventriculotomy aneurysm and left ventricular dysfunction following closed mitral commissurotomy. A ventricular aneurysm at the site of a previous transventricular commissurotomy for mitral stenosis was diagnosed in a 39 year old female. Catheterization data showed abnormal left ventricular response to exercise and to increased afterload. Death occurred following mitral valve replacement. At autopsy no cause other than left ventricular aneurysm could be established to explain left ventricular dysfunction."}
{"id": "PMID:1084669", "title": "[Calcific constrictive pericarditis of anginal form complicated by hemopericardium and tamponade during anticoagulant treatment].", "content": "The case-history of a patient with calcified constrictive epicardopericarditis with rapidely progressive aggravation after digestive hemorrage due to excessive dose or oral anticoagulant therapy is reported. The occurrence of a cardiac tamponade has necessitated pericardectomy which has permitted to detect a partitionned hemopericardium. The surgical act was followed by gross improvement of both constrictive phenomenon and angina pectoris which have grounded the prescription of anticoagulant therapy. Anticoagulant therapy is likely responsible of the hemopericardium. Such facts are exceptionals in the course of constrictive pericarditis; diagnostic and therapeutic problems related to this complication are studied. The question of angina pectoris in the course of acute and chronic pericarditis is briefly studied by the way of this observation. Attention is drawed on the danger of the prescription of anticoagulant therapy in the course of some constrictive pericarditides.", "contents": "[Calcific constrictive pericarditis of anginal form complicated by hemopericardium and tamponade during anticoagulant treatment]. The case-history of a patient with calcified constrictive epicardopericarditis with rapidely progressive aggravation after digestive hemorrage due to excessive dose or oral anticoagulant therapy is reported. The occurrence of a cardiac tamponade has necessitated pericardectomy which has permitted to detect a partitionned hemopericardium. The surgical act was followed by gross improvement of both constrictive phenomenon and angina pectoris which have grounded the prescription of anticoagulant therapy. Anticoagulant therapy is likely responsible of the hemopericardium. Such facts are exceptionals in the course of constrictive pericarditis; diagnostic and therapeutic problems related to this complication are studied. The question of angina pectoris in the course of acute and chronic pericarditis is briefly studied by the way of this observation. Attention is drawed on the danger of the prescription of anticoagulant therapy in the course of some constrictive pericarditides."}
{"id": "PMID:1084670", "title": "Acute hemorrhagic gastritis--diagnosis and treatment.", "content": "Approximately 10-20% of patients admitted with acute gastrointestinal bleeding suffer from hemorrhagic erosive gastritis. A material of 36 patients is presented and the literature reviewed. The moderate bleeders stop bleeding within hours to a few days and the majority may safely be managed conservatively, i.e. with blood transfusions. The severe bleeders continue to bleed for a long time and surgery is often required. Different operative procedures have been suggested. The mortality in this group of patients is high--nearly 30%, and the frequency of postoperative rebleeding is also high. It is concluded that there is no absolute operative procedure of choice but vagotomy combined with subtotal gastrectomy offer the best protection against rebleeding. Treatment with selective application of vasoconstrictors is mentioned.", "contents": "Acute hemorrhagic gastritis--diagnosis and treatment. Approximately 10-20% of patients admitted with acute gastrointestinal bleeding suffer from hemorrhagic erosive gastritis. A material of 36 patients is presented and the literature reviewed. The moderate bleeders stop bleeding within hours to a few days and the majority may safely be managed conservatively, i.e. with blood transfusions. The severe bleeders continue to bleed for a long time and surgery is often required. Different operative procedures have been suggested. The mortality in this group of patients is high--nearly 30%, and the frequency of postoperative rebleeding is also high. It is concluded that there is no absolute operative procedure of choice but vagotomy combined with subtotal gastrectomy offer the best protection against rebleeding. Treatment with selective application of vasoconstrictors is mentioned."}
{"id": "PMID:1084671", "title": "[T-lymphocyte subpopulation in the blood of patients with chronic lymphocytic leukemia].", "content": "In 40 healthy subjects and 80 patients with chronic lymphocytic leukaemia (CLL) rosette formation test with sheep erythrocytes subjected to the action of neuraminidase (nRTB), immunofluorescent investigation of membrane immunoglobulins (BIg) and a combined test (nRTB + BIg) were performed. The relative number of rosette-forming cells (T lymphocytes) was much lower in the group of CLL patients (0.16 +/- 0.08 1/1) than in controls (0.64 +/- 0.12 1/1). The absolute number of T lymphocytes (BLLT) was, however, significantly higher in patients with CLL 5.1 +/- 4.4 G/L) than in controls (0.98 +/- 0.28 G/L). The relative number of bireceptor cells was 0.0045 +/- 0.0093 1/1 in CLL patients and that of null-cells 0.24 +/- 0.17 1/1. Analogous values in the control group were 0.0089 +/- 0.009 1/1 and 0.21 +/- 0.091 1/1. The possible causes of this rise in the absolute number of T lymphocytes in CLL may by: 1) proliferation of both subpopulations of lymphocytes, 2) reaction of T lymphocytes against leukaemic cells, 3) disturbances of equilibrium between B and T lymphocytes.", "contents": "[T-lymphocyte subpopulation in the blood of patients with chronic lymphocytic leukemia]. In 40 healthy subjects and 80 patients with chronic lymphocytic leukaemia (CLL) rosette formation test with sheep erythrocytes subjected to the action of neuraminidase (nRTB), immunofluorescent investigation of membrane immunoglobulins (BIg) and a combined test (nRTB + BIg) were performed. The relative number of rosette-forming cells (T lymphocytes) was much lower in the group of CLL patients (0.16 +/- 0.08 1/1) than in controls (0.64 +/- 0.12 1/1). The absolute number of T lymphocytes (BLLT) was, however, significantly higher in patients with CLL 5.1 +/- 4.4 G/L) than in controls (0.98 +/- 0.28 G/L). The relative number of bireceptor cells was 0.0045 +/- 0.0093 1/1 in CLL patients and that of null-cells 0.24 +/- 0.17 1/1. Analogous values in the control group were 0.0089 +/- 0.009 1/1 and 0.21 +/- 0.091 1/1. The possible causes of this rise in the absolute number of T lymphocytes in CLL may by: 1) proliferation of both subpopulations of lymphocytes, 2) reaction of T lymphocytes against leukaemic cells, 3) disturbances of equilibrium between B and T lymphocytes."}
{"id": "PMID:1084673", "title": "B, T and null lymphocytes in newborn infants and their mothers.", "content": "Estimation of B, T and null cells were performed on 29 newborn healthy babies and 16 mothers. The lymphocytes were isolated from peripheral venous blood, which is considered to be more representative of the immune state in the newborn than the cord blood. B lymphocytes were estimated by cytofluorometric measurements, T lymphocytes by sheep red blood cell rosette technique, (SRBC-R). Combined immunofluorescence and SRBC-R technique revealed the null cells. In the newborn babies the amount of B and T cells were found to be diminished. In the mothers the amount of B lymphocytes were low compared with normal adults. The rather high null cell percentage found in the babies might represent immature precursor cells. Mothers seem to be immuno-depressed as reflected in the low amount of B cells.", "contents": "B, T and null lymphocytes in newborn infants and their mothers. Estimation of B, T and null cells were performed on 29 newborn healthy babies and 16 mothers. The lymphocytes were isolated from peripheral venous blood, which is considered to be more representative of the immune state in the newborn than the cord blood. B lymphocytes were estimated by cytofluorometric measurements, T lymphocytes by sheep red blood cell rosette technique, (SRBC-R). Combined immunofluorescence and SRBC-R technique revealed the null cells. In the newborn babies the amount of B and T cells were found to be diminished. In the mothers the amount of B lymphocytes were low compared with normal adults. The rather high null cell percentage found in the babies might represent immature precursor cells. Mothers seem to be immuno-depressed as reflected in the low amount of B cells."}
{"id": "PMID:1084674", "title": "Response of human lymphocytes to mitogenic stimuli after irradiation in vitro.", "content": "Human lymphocytes were exposed to varying doses of roentgen irradiation in vitro and thereafter tested for reactivity to different polyclonal mitogens and antigens using DNA synthesis as a marker for viability. The dose response profiles obtained indicate that there are two subpopulations of lymphocytes which are responsive to phytohaemagglutinin, poke weed mitogen, concanavalin A and allogeneic cells. One is relatively sensitive to radiation and the other is relatively resistant. However, no \"resistant\" PPD-tuberculin responsive cell population could be detected. Irradiated lymphocyte populations enriched for T-cells exhibited both a sensitive and a resistant PHA-responsive population, whereas cell populations enriched for B-cells only exhibited a radiation sensitive one.", "contents": "Response of human lymphocytes to mitogenic stimuli after irradiation in vitro. Human lymphocytes were exposed to varying doses of roentgen irradiation in vitro and thereafter tested for reactivity to different polyclonal mitogens and antigens using DNA synthesis as a marker for viability. The dose response profiles obtained indicate that there are two subpopulations of lymphocytes which are responsive to phytohaemagglutinin, poke weed mitogen, concanavalin A and allogeneic cells. One is relatively sensitive to radiation and the other is relatively resistant. However, no \"resistant\" PPD-tuberculin responsive cell population could be detected. Irradiated lymphocyte populations enriched for T-cells exhibited both a sensitive and a resistant PHA-responsive population, whereas cell populations enriched for B-cells only exhibited a radiation sensitive one."}
{"id": "PMID:1084675", "title": "Reisolation and characterization of Haemophilus influenzae-murium.", "content": "Twenty-three Haemophilus influenzae-murium strains were isolated from the nasopharynx of healthy CFLP white mice. Microbiological characterization of the cultures confirmed the taxonomic position of the organisms which had become problematic due to the loss of the original isolates. The type strain proposed, EO1, is available from the Hungarian National Collection of Medical Bacteria, National Institute of Public Health, Budapest as 95001.", "contents": "Reisolation and characterization of Haemophilus influenzae-murium. Twenty-three Haemophilus influenzae-murium strains were isolated from the nasopharynx of healthy CFLP white mice. Microbiological characterization of the cultures confirmed the taxonomic position of the organisms which had become problematic due to the loss of the original isolates. The type strain proposed, EO1, is available from the Hungarian National Collection of Medical Bacteria, National Institute of Public Health, Budapest as 95001."}
{"id": "PMID:1084676", "title": "Perceptions of the use of drugs--a cross-sectional analysis.", "content": "Samples of six specific groups were surveyed regarding their differential perceptions of the use of drugs. Current use was discussed in terms of comparisons of different groups' ratings of each group, comparisons of differential use of drugs by each group and comparisons between each groups' perception of its own use and its perception of use by other groups.", "contents": "Perceptions of the use of drugs--a cross-sectional analysis. Samples of six specific groups were surveyed regarding their differential perceptions of the use of drugs. Current use was discussed in terms of comparisons of different groups' ratings of each group, comparisons of differential use of drugs by each group and comparisons between each groups' perception of its own use and its perception of use by other groups."}
{"id": "PMID:1084678", "title": "Purification of kallikrein from cat submaxillary gland.", "content": "Cat submaxillary kallikrein was purified from the water extract of the gland by 50-75% acetone fractionation, DEAE-Sephadex A-50 chromatography, Sephadex G-75 gel filtration and Ampholine isoelectric focusing. The final preparation gave the activities of 1260 KU/mg in vasodilator assay, 24.5 and 11.6 units/mg on BAEE and TAME esterolytic assay, respectively. Trasylol gave only weak inhibitory effect on this kallikrein. The isoelectric points of the kallikrein were in the range pH 4.2-5.0, and this kallikrein seemed to consist of several multiple forms with different pI values, like some other glandular kallikreins. It gave a single protein band in disc electrophoresis, while it migrated in retard and formed a broad one. This may be ascribable to the rich carbohydrate content in the kallikrein.", "contents": "Purification of kallikrein from cat submaxillary gland. Cat submaxillary kallikrein was purified from the water extract of the gland by 50-75% acetone fractionation, DEAE-Sephadex A-50 chromatography, Sephadex G-75 gel filtration and Ampholine isoelectric focusing. The final preparation gave the activities of 1260 KU/mg in vasodilator assay, 24.5 and 11.6 units/mg on BAEE and TAME esterolytic assay, respectively. Trasylol gave only weak inhibitory effect on this kallikrein. The isoelectric points of the kallikrein were in the range pH 4.2-5.0, and this kallikrein seemed to consist of several multiple forms with different pI values, like some other glandular kallikreins. It gave a single protein band in disc electrophoresis, while it migrated in retard and formed a broad one. This may be ascribable to the rich carbohydrate content in the kallikrein."}
{"id": "PMID:1084679", "title": "Prekallikein and kallikrein inhibitor in liver cirrhosis and hepatitis.", "content": "Plasma prekallikrein (kallikreinogen) and kallikrein inhibitor, assayed with the kaolin activable esterase method, have been evaluated in 20 patients with hepatic cirrhosis, in 12 cases with jaundice from acute viral hepatitis, and in 9 normal. A significant reduction of the plasma prekallikrein in cirrhosis has been found. A lowering of plasma prekallikrein has also been observed in viral hepatitis; in this condition, however, the modifications were less important than those obtained in cirrhosis. In three cases of hepatitis, the behaviour of the plasma prekallikrein and kallikrein inhibitor have been controlled during the period of the disease and compared with the behaviour of some conventional parameters, such as serum transaminases and bilirubin. An important increase of the prekallikrein level has been observed during the improvement of hepatitis. These data confirm the implication of the prekallikrein-kallikrein system in severe liver diseases, and indirectly points out the role of the liver in maintaining the physiological balance of the kallikrein system.", "contents": "Prekallikein and kallikrein inhibitor in liver cirrhosis and hepatitis. Plasma prekallikrein (kallikreinogen) and kallikrein inhibitor, assayed with the kaolin activable esterase method, have been evaluated in 20 patients with hepatic cirrhosis, in 12 cases with jaundice from acute viral hepatitis, and in 9 normal. A significant reduction of the plasma prekallikrein in cirrhosis has been found. A lowering of plasma prekallikrein has also been observed in viral hepatitis; in this condition, however, the modifications were less important than those obtained in cirrhosis. In three cases of hepatitis, the behaviour of the plasma prekallikrein and kallikrein inhibitor have been controlled during the period of the disease and compared with the behaviour of some conventional parameters, such as serum transaminases and bilirubin. An important increase of the prekallikrein level has been observed during the improvement of hepatitis. These data confirm the implication of the prekallikrein-kallikrein system in severe liver diseases, and indirectly points out the role of the liver in maintaining the physiological balance of the kallikrein system."}
{"id": "PMID:1084681", "title": "Histamine and kallikrein system in man.", "content": "Histamine infusion modifies the kallikrein system, studied by kaolin contact method, in man. The main modifications are the increase of the spontaneous esterase activity and the prekallikrein lowering. Apparently the histamine administration activates the kallikrein, and consequently a release of kinin can take place.", "contents": "Histamine and kallikrein system in man. Histamine infusion modifies the kallikrein system, studied by kaolin contact method, in man. The main modifications are the increase of the spontaneous esterase activity and the prekallikrein lowering. Apparently the histamine administration activates the kallikrein, and consequently a release of kinin can take place."}
{"id": "PMID:1084682", "title": "Plasmatic prekallikrein and kallikrein inhibitor in pregnancy, labor and in newborn.", "content": "In order to reveal the possible physiological role of the kallikrein during the stress of birth, the prekallikrein and kallikrein inhibitor were evaluated in 15 pregnant women and in 15 newborns from terminated pregnancies. The results show a very low level of prekallikrein in the funiculus blood at the moment of birth and a still lower level in venous blood 24 hours after birth. No important changes resulted in the mother's blood either in pregnancy, nor at the end of the labour.", "contents": "Plasmatic prekallikrein and kallikrein inhibitor in pregnancy, labor and in newborn. In order to reveal the possible physiological role of the kallikrein during the stress of birth, the prekallikrein and kallikrein inhibitor were evaluated in 15 pregnant women and in 15 newborns from terminated pregnancies. The results show a very low level of prekallikrein in the funiculus blood at the moment of birth and a still lower level in venous blood 24 hours after birth. No important changes resulted in the mother's blood either in pregnancy, nor at the end of the labour."}
{"id": "PMID:1084684", "title": "Monitoring tissue oxygenation of the heart after myocardial revascularization.", "content": "A polarographic technique capable of simultaneous monitoring of myocardial tissue oxygen tension (MPO2) and intramyocardial electrograms by way of the same electrodes has been developed. Initially, the method was evaluated in dogs to verify the appropriateness of the directional changes of MPO2 in function of selected determinants of myocardial oxygen supply (regional coronary blood flow, arterial blood oxygen tension) and demand (heart rate, force of ventricular contraction). A combined reduction of MPO2 and elevation of the S-T segment in the corresponding electrograms was observed only when a 50 percent or greater reduction of blood flow to the sampled area was effected. Subsequently, in nine patients undergoing aortocoronary bypass surgery, MPO2 was measured from 48 areas for 2 weeks postoperatively. In 11 normal and 31 revascularized areas, MPO2 increased during the postoperative period. In four areas subsequently found to be supplied by occluded grafts MPO2 decreased from 12.7 +/- 3.1 (mean +/- standard error) to 10.1 +/- 3.3 mm Hg (P less than 0.05). In two areas, MPO2 decreased during the 3rd postoperative day from 16 to 3 and from 14 to 4.2 mm Hg, respectively. This reduction was attended by a significant rise in the S-T segment of the corresponding electrograms. This finding preceded by 24 hours standard electrocardiographic evidence of myocardial infarction. This technique appears to be sensitive and reliable, and thereby capable of enhancing the management of patients during the high risk early postoperative period after coronary bypass surgery.", "contents": "Monitoring tissue oxygenation of the heart after myocardial revascularization. A polarographic technique capable of simultaneous monitoring of myocardial tissue oxygen tension (MPO2) and intramyocardial electrograms by way of the same electrodes has been developed. Initially, the method was evaluated in dogs to verify the appropriateness of the directional changes of MPO2 in function of selected determinants of myocardial oxygen supply (regional coronary blood flow, arterial blood oxygen tension) and demand (heart rate, force of ventricular contraction). A combined reduction of MPO2 and elevation of the S-T segment in the corresponding electrograms was observed only when a 50 percent or greater reduction of blood flow to the sampled area was effected. Subsequently, in nine patients undergoing aortocoronary bypass surgery, MPO2 was measured from 48 areas for 2 weeks postoperatively. In 11 normal and 31 revascularized areas, MPO2 increased during the postoperative period. In four areas subsequently found to be supplied by occluded grafts MPO2 decreased from 12.7 +/- 3.1 (mean +/- standard error) to 10.1 +/- 3.3 mm Hg (P less than 0.05). In two areas, MPO2 decreased during the 3rd postoperative day from 16 to 3 and from 14 to 4.2 mm Hg, respectively. This reduction was attended by a significant rise in the S-T segment of the corresponding electrograms. This finding preceded by 24 hours standard electrocardiographic evidence of myocardial infarction. This technique appears to be sensitive and reliable, and thereby capable of enhancing the management of patients during the high risk early postoperative period after coronary bypass surgery."}
{"id": "PMID:1084685", "title": "Gastrointestinal bleeding and iron absorption in the experimental blind loop syndrome.", "content": "Rats with surgically created self-filling jejunal blind loops and the blind loop syndrome manifested gastrointestinal bleeding and hyperabsorption of iron. Although the mean hematocrit and serum iron levels of rats with self-filling blind loops became overtly anemic and manifested low-serum iron levels. It is suggested that the documented gastrointestinal bleeding in these rats with the experimental blind loop syndrome is another manifestation of damage to the intestinal epithelium in conditions of small intestinal bacterial overgrowth.", "contents": "Gastrointestinal bleeding and iron absorption in the experimental blind loop syndrome. Rats with surgically created self-filling jejunal blind loops and the blind loop syndrome manifested gastrointestinal bleeding and hyperabsorption of iron. Although the mean hematocrit and serum iron levels of rats with self-filling blind loops became overtly anemic and manifested low-serum iron levels. It is suggested that the documented gastrointestinal bleeding in these rats with the experimental blind loop syndrome is another manifestation of damage to the intestinal epithelium in conditions of small intestinal bacterial overgrowth."}
{"id": "PMID:1084686", "title": "Immunologic and electronmicroscopic characteristics of a case of immunoblastic lymphadenopathy.", "content": "A new disease entity of the lymphoid system has recently been reported by Lukes and Tindle as immunoblastic lymphadenopathy and by Frizzera and associates as angioimmunoblastic lymphadenopathy with dysproteinemia. Reported herein are cytologic, histologic, ultrastructural and immunologic studies of several tissues of a patient with this disorder. In addition to confirming the previous observations that the main cellular constituents in the affected tissue consist of immunoblast, plasmacytoid lymphocytes and plasma cells, the authors have demonstrated a profound deficiency of T lymphocytes in this patient. The previously described amorphous interstitial material appears to represent multiple small cytoplasmic fragments. The histiocytic component, which appears in variable amounts in this disease, in the reported case was very active in phagocytizing cellular and nuclear debris, some of which appeared to be lymphocytic in origin. A rubella infection preceded the onset of the disorder by 3 months.", "contents": "Immunologic and electronmicroscopic characteristics of a case of immunoblastic lymphadenopathy. A new disease entity of the lymphoid system has recently been reported by Lukes and Tindle as immunoblastic lymphadenopathy and by Frizzera and associates as angioimmunoblastic lymphadenopathy with dysproteinemia. Reported herein are cytologic, histologic, ultrastructural and immunologic studies of several tissues of a patient with this disorder. In addition to confirming the previous observations that the main cellular constituents in the affected tissue consist of immunoblast, plasmacytoid lymphocytes and plasma cells, the authors have demonstrated a profound deficiency of T lymphocytes in this patient. The previously described amorphous interstitial material appears to represent multiple small cytoplasmic fragments. The histiocytic component, which appears in variable amounts in this disease, in the reported case was very active in phagocytizing cellular and nuclear debris, some of which appeared to be lymphocytic in origin. A rubella infection preceded the onset of the disorder by 3 months."}
{"id": "PMID:1084688", "title": "alpha1-Antitrypsin deficiency and liver disease in children.", "content": "This report describes the clinical, biochemical, and hepatic morphologic findings in ten children with severe serum alpha1-antitrypsin deficiency. Genetic protease inhibitor (Pi) phenotyping, using acid-starch gel and crossed antigen-antibody electrophoresis, demonstrated Pi phenotype ZZ in all our cases. In eight patients, manifestations of liver disease appeared during the first year of life. The case reports show that alpha1-antitrypsin deficiency should be suspected in any child with neonatal hepatitis, unexplained hepatomegaly or splenomegaly, or cirrhosis. In our report, one infant is normal at age 6 months, and one infant had progressive hepatic damage that culminated in liver failure and death at age 6 months. The variable clinical course and prognosis for infants with severe alpha1-antitrypsin deficiency is well illustrated by these two infants.", "contents": "alpha1-Antitrypsin deficiency and liver disease in children. This report describes the clinical, biochemical, and hepatic morphologic findings in ten children with severe serum alpha1-antitrypsin deficiency. Genetic protease inhibitor (Pi) phenotyping, using acid-starch gel and crossed antigen-antibody electrophoresis, demonstrated Pi phenotype ZZ in all our cases. In eight patients, manifestations of liver disease appeared during the first year of life. The case reports show that alpha1-antitrypsin deficiency should be suspected in any child with neonatal hepatitis, unexplained hepatomegaly or splenomegaly, or cirrhosis. In our report, one infant is normal at age 6 months, and one infant had progressive hepatic damage that culminated in liver failure and death at age 6 months. The variable clinical course and prognosis for infants with severe alpha1-antitrypsin deficiency is well illustrated by these two infants."}
{"id": "PMID:1084690", "title": "The radiology corner: metastasis to the colon.", "content": "This case serves to illustrate the multiple routes which may occur in the dissemination of carcinoma of the stomach of the large bowel. Spread along the gastrocolic ligament involves the superior aspect of the transverse colon with limitation laterally by the phrenicocolic ligament. Once ascites has developed there is spread along the superior border of the sigmoid and lateral margin of the right side of the colon.", "contents": "The radiology corner: metastasis to the colon. This case serves to illustrate the multiple routes which may occur in the dissemination of carcinoma of the stomach of the large bowel. Spread along the gastrocolic ligament involves the superior aspect of the transverse colon with limitation laterally by the phrenicocolic ligament. Once ascites has developed there is spread along the superior border of the sigmoid and lateral margin of the right side of the colon."}
{"id": "PMID:1084691", "title": "Comparison of longitudinal and cross-sectional analysis: maternal age and stillbirth ratio.", "content": "Previous studies have reported a U or J shaped association between stillbirth ratio and maternal age and have led to attempts to determine the causes behind the association. These analyses have generally been based on cross-sectional data. This study contrasts the descriptions of the association resulting from cross-sectional and the more appropriate longitudinal analysis of the same body of data. It is concluded that cross-sectional analysis substantially underestimates the maternal age of which stillbirth ratio reaches a minimum for each birth order examined. Additionally, the basic shape of the relationship is sometimes altered. Weighted logistic polynomial regression is used to describe the associations. Traditional interpretations of the results of a cross-sectional analysis have been that women who postpone childbearing until the late twenties thereby increase their risk of stillbirth. A consequence of the present findings is the rejection of the claim that women who choose employment during the period of their early twenties automatically incur elevated risk of stillbirth in their postponed pregnancies.", "contents": "Comparison of longitudinal and cross-sectional analysis: maternal age and stillbirth ratio. Previous studies have reported a U or J shaped association between stillbirth ratio and maternal age and have led to attempts to determine the causes behind the association. These analyses have generally been based on cross-sectional data. This study contrasts the descriptions of the association resulting from cross-sectional and the more appropriate longitudinal analysis of the same body of data. It is concluded that cross-sectional analysis substantially underestimates the maternal age of which stillbirth ratio reaches a minimum for each birth order examined. Additionally, the basic shape of the relationship is sometimes altered. Weighted logistic polynomial regression is used to describe the associations. Traditional interpretations of the results of a cross-sectional analysis have been that women who postpone childbearing until the late twenties thereby increase their risk of stillbirth. A consequence of the present findings is the rejection of the claim that women who choose employment during the period of their early twenties automatically incur elevated risk of stillbirth in their postponed pregnancies."}
{"id": "PMID:1084692", "title": "Marked cyclic leukocytosis-leukopenia in chronic myelogenous leukemia.", "content": "A spontaneous oscillation of the white blood cell count was observed in a 58 year old man with chronic myelogenous leukemia (CML). Similar cyclic variations were noted in the platelet and reticulocyte counts with no apparent alterations in marrow cellularity to account for such changes. Since direct correlation was noted between white blood cells, platelets, and reticulocyte counts versus spleen size, it suggests that splenic hemopoiesis may be responsible for these cyclic changes. A possible inverse relationship between colony-stimulating factor (CSF) activity and the white blood cell count was noted, suggesting that CSF may be the humoral agent controlling granulocyte production. A direct correlation between the white blood cell count and serum unsaturated vitamin B12 binding capacity (UBBC) and lysozyme was also noted and further supports the concept that the latter two are measures of the granulocyte pool and metabolism. An inverse relationship between CSF activity and the UBBC suggests that these may be two different entities. Finally a modified form of standard chemotherapy may be effective in inducing remission in cases of CML with marked cyclic leukocytosis-leukopenia.", "contents": "Marked cyclic leukocytosis-leukopenia in chronic myelogenous leukemia. A spontaneous oscillation of the white blood cell count was observed in a 58 year old man with chronic myelogenous leukemia (CML). Similar cyclic variations were noted in the platelet and reticulocyte counts with no apparent alterations in marrow cellularity to account for such changes. Since direct correlation was noted between white blood cells, platelets, and reticulocyte counts versus spleen size, it suggests that splenic hemopoiesis may be responsible for these cyclic changes. A possible inverse relationship between colony-stimulating factor (CSF) activity and the white blood cell count was noted, suggesting that CSF may be the humoral agent controlling granulocyte production. A direct correlation between the white blood cell count and serum unsaturated vitamin B12 binding capacity (UBBC) and lysozyme was also noted and further supports the concept that the latter two are measures of the granulocyte pool and metabolism. An inverse relationship between CSF activity and the UBBC suggests that these may be two different entities. Finally a modified form of standard chemotherapy may be effective in inducing remission in cases of CML with marked cyclic leukocytosis-leukopenia."}
{"id": "PMID:1084693", "title": "Massive colonic bleeding and oral contraceptive \"pills\".", "content": "Two patients on oral contraceptives who developed massive colonic bleeding have been presented. The clinical course and various studies suggest the mesenteric insufficiency syndrome as being a possible result of oral contraceptive effect on mesenteric vasculature. Although no definite histologic confirmation is available, the onset, course, and follow-up of these patients suggest contraceptive-induced massive colonic bleeding. The spectrum of oral contraceptive-induced colonic bleeding may vary from mucosal injury with massive colonic bleeding to transmural infarction. Only the clinical follow-up would determine the severity of the involvement and that would determine whether surgical intervention  will be necessary.", "contents": "Massive colonic bleeding and oral contraceptive \"pills\". Two patients on oral contraceptives who developed massive colonic bleeding have been presented. The clinical course and various studies suggest the mesenteric insufficiency syndrome as being a possible result of oral contraceptive effect on mesenteric vasculature. Although no definite histologic confirmation is available, the onset, course, and follow-up of these patients suggest contraceptive-induced massive colonic bleeding. The spectrum of oral contraceptive-induced colonic bleeding may vary from mucosal injury with massive colonic bleeding to transmural infarction. Only the clinical follow-up would determine the severity of the involvement and that would determine whether surgical intervention  will be necessary."}
{"id": "PMID:1084694", "title": "Miotic drugs: a review of ocular, visual, and systemic complications.", "content": "Cholinergic drugs and anticholinesterases have caused ocular complications including cataracts, iris cysts, retinal detachment, iritis, increased intraocular tension, allergic and toxic reactions, and hypermia. The visual complications include accommodative spasm; myopia and reduced visual acuity; changes in vision sensitivity, dark adaptation, and visual fields; and discomfort. The systemic complications are those common to cholinergic drugs and anticholinesterases. This review is intended as a ready reference to the drugs that cause such effects and source material.", "contents": "Miotic drugs: a review of ocular, visual, and systemic complications. Cholinergic drugs and anticholinesterases have caused ocular complications including cataracts, iris cysts, retinal detachment, iritis, increased intraocular tension, allergic and toxic reactions, and hypermia. The visual complications include accommodative spasm; myopia and reduced visual acuity; changes in vision sensitivity, dark adaptation, and visual fields; and discomfort. The systemic complications are those common to cholinergic drugs and anticholinesterases. This review is intended as a ready reference to the drugs that cause such effects and source material."}
{"id": "PMID:1084695", "title": "The development of the Generation II as an aid for patients with retinitis pigmentosa and night blindness.", "content": "The Generation II is a modification of the Army's night photography/observation instrumentaton. Low levels of light are intensified approximately 700 times to stimulate cone function. The individual with no rod function is able to see at night under these intensified amounts of illumination. The device can be used for night \"spotting and mobility\" purposes, much like a telescope is used during the day by the partially-sighted with intact rod function and poor cone function. This paper is presented to inform the optometrist of the availability and usefullness of this device for his patients.", "contents": "The development of the Generation II as an aid for patients with retinitis pigmentosa and night blindness. The Generation II is a modification of the Army's night photography/observation instrumentaton. Low levels of light are intensified approximately 700 times to stimulate cone function. The individual with no rod function is able to see at night under these intensified amounts of illumination. The device can be used for night \"spotting and mobility\" purposes, much like a telescope is used during the day by the partially-sighted with intact rod function and poor cone function. This paper is presented to inform the optometrist of the availability and usefullness of this device for his patients."}
{"id": "PMID:1084696", "title": "Clinical observation of posterior polymorphous dystrophy.", "content": "The clinical picture of this dominant corneal endothelial dystrophy is illustrated with a series of case histories. Differential diagnosis is considered both from the clinical manifestations and from the histopathological evaluation of degenerate corneal tissue.", "contents": "Clinical observation of posterior polymorphous dystrophy. The clinical picture of this dominant corneal endothelial dystrophy is illustrated with a series of case histories. Differential diagnosis is considered both from the clinical manifestations and from the histopathological evaluation of degenerate corneal tissue."}
{"id": "PMID:1084697", "title": "Genetic control of the immune response to poly(Glu52Lys33Tyr15) in neonatally thymectomized high and low responder rats.", "content": "The immune response to poly (Glu52Lys33Tyr15) is under polygenic control and linked to the major histocompatibility complex of the rat. Aggregation of this antigen with methylated bovine serum albumin (MeBSA) eliminates the expression of genetic control by increasing the response of low responders and decreasing that of high responders. Humoral and cellular aspects of the immune response to both unaggregated and aggregated poly (Glu52Lys33Tyr15) were investigated in neonatally thymectomized high-responder ACI and low-responder F344 rats. T cells are necessary for responses to unaggregated poly (Glu52Lys33Tyr15) since thymectomy significantly decreased numbers of antibody-forming cells and serum antibody levels, and delayed hypersensitivity responses and antigen-induced in vitro proliferation. However, thymectomy had no significant effect on these parameters of immune responsiveness in either ACI or F344 rats immunized with poly (Glu52Lys33Tyr15)/MeBSA. Aggregation also increased IgG production and delayed hypersensitivity and antibody affinity in low responders.", "contents": "Genetic control of the immune response to poly(Glu52Lys33Tyr15) in neonatally thymectomized high and low responder rats. The immune response to poly (Glu52Lys33Tyr15) is under polygenic control and linked to the major histocompatibility complex of the rat. Aggregation of this antigen with methylated bovine serum albumin (MeBSA) eliminates the expression of genetic control by increasing the response of low responders and decreasing that of high responders. Humoral and cellular aspects of the immune response to both unaggregated and aggregated poly (Glu52Lys33Tyr15) were investigated in neonatally thymectomized high-responder ACI and low-responder F344 rats. T cells are necessary for responses to unaggregated poly (Glu52Lys33Tyr15) since thymectomy significantly decreased numbers of antibody-forming cells and serum antibody levels, and delayed hypersensitivity responses and antigen-induced in vitro proliferation. However, thymectomy had no significant effect on these parameters of immune responsiveness in either ACI or F344 rats immunized with poly (Glu52Lys33Tyr15)/MeBSA. Aggregation also increased IgG production and delayed hypersensitivity and antibody affinity in low responders."}
{"id": "PMID:1084698", "title": "alpha- and beta-adrenergic receptors in regulation of ionic transport in frog cornea.", "content": "The effect of catecholamines and adrenergic blocking agents on the transport of Cl- and Na+ of the isolated frog cornea was studied. Cl- transport was stimulated by the three catecholamines tested at 10(-6) to 10(-5) M concentration. The stimulation produced by 10(-6) M epinephrine was partially inhibited by both the alpha-blocker phentolamine and the beta blocker propranolol. The stimulation produced by 10(-6) M isoproterenol was not affected by 10(-5) M phentolamine but was completely prevented by 10(-5) M propranolol. The stimulation produced by 10(-5) M norepinephrine was completely blocked by the 10(-5) M propranolol and partially by 10(-5) M phentolamine. Catecholamines had no effect on the normally small Na+ transport. When the Na+ transport was previously increased by amphotericin B, epinephrine and isoproterenol produced a small and inconsistent stimulatory effect. Norepinephrine was without effect. It is concluded that both alpha- and beta-adrenergic receptors are present in the frog cornea and that the Cl- transport is increased by the stimulation of either receptor. The minimal effect on the Na+ transport further suggests the separation of pathways of the Cl- and Na+ transport in the frog cornea.", "contents": "alpha- and beta-adrenergic receptors in regulation of ionic transport in frog cornea. The effect of catecholamines and adrenergic blocking agents on the transport of Cl- and Na+ of the isolated frog cornea was studied. Cl- transport was stimulated by the three catecholamines tested at 10(-6) to 10(-5) M concentration. The stimulation produced by 10(-6) M epinephrine was partially inhibited by both the alpha-blocker phentolamine and the beta blocker propranolol. The stimulation produced by 10(-6) M isoproterenol was not affected by 10(-5) M phentolamine but was completely prevented by 10(-5) M propranolol. The stimulation produced by 10(-5) M norepinephrine was completely blocked by the 10(-5) M propranolol and partially by 10(-5) M phentolamine. Catecholamines had no effect on the normally small Na+ transport. When the Na+ transport was previously increased by amphotericin B, epinephrine and isoproterenol produced a small and inconsistent stimulatory effect. Norepinephrine was without effect. It is concluded that both alpha- and beta-adrenergic receptors are present in the frog cornea and that the Cl- transport is increased by the stimulation of either receptor. The minimal effect on the Na+ transport further suggests the separation of pathways of the Cl- and Na+ transport in the frog cornea."}
{"id": "PMID:1084699", "title": "Inhibition of H+ secretion in the frog gastric mucosa by ATP and related compounds.", "content": "Addition of adenosine triphosphate (ATP) to the nutrient (submucosal-facing) solution of the histamine-stimulated in vitro frog (Rana pipiens) gastric mucosa produces a marked reduction in the H+ secretory rate and an increase in transmucosal potential difference (PD) and resistance in both Cl- and Cl-free media. The effects are reversible upon removal of ATP. The threshold concentration is between 1 and 2 mM, and 5 mM produce maximal inhibition. It is shown that the effects of ATP are not due to a change in pH or osmolarity of the nutrient fluid, or to a decrease in the Ca2+ and/or Mg2+ activities of the nutrient fluid. It is also shown that the inhibitory action of ATP is not dependent on a chelation complex between Ca2+ or Mg2+. Adenosine diphosphate also produces effects essentially the same as ATP whereas 5'-adenosine monophosphate and adenosine produce relatively little or no change.", "contents": "Inhibition of H+ secretion in the frog gastric mucosa by ATP and related compounds. Addition of adenosine triphosphate (ATP) to the nutrient (submucosal-facing) solution of the histamine-stimulated in vitro frog (Rana pipiens) gastric mucosa produces a marked reduction in the H+ secretory rate and an increase in transmucosal potential difference (PD) and resistance in both Cl- and Cl-free media. The effects are reversible upon removal of ATP. The threshold concentration is between 1 and 2 mM, and 5 mM produce maximal inhibition. It is shown that the effects of ATP are not due to a change in pH or osmolarity of the nutrient fluid, or to a decrease in the Ca2+ and/or Mg2+ activities of the nutrient fluid. It is also shown that the inhibitory action of ATP is not dependent on a chelation complex between Ca2+ or Mg2+. Adenosine diphosphate also produces effects essentially the same as ATP whereas 5'-adenosine monophosphate and adenosine produce relatively little or no change."}
{"id": "PMID:1084700", "title": "Snake venom ophthalmia and blindness caused by the spitting cobra (Naja nigricollis) in Nigeria.", "content": "Venom entered the eyes of 9 patients spat at by the spitting cobra, Naja nigricollis. In 5 the only effect was a simple conjunctivitis but 4 had corneal ulceration, 1 developed anterior uveitis indicating absorption of venom in the anterior chamber, and 2 were permanently blinded. Treatment of this rare emergency is discussed: immediate irrigation of the eye with water, careful examination for corneal abrasion, and prevention of secondary infection are recommended. The value of local specific antivenom is unproven.", "contents": "Snake venom ophthalmia and blindness caused by the spitting cobra (Naja nigricollis) in Nigeria. Venom entered the eyes of 9 patients spat at by the spitting cobra, Naja nigricollis. In 5 the only effect was a simple conjunctivitis but 4 had corneal ulceration, 1 developed anterior uveitis indicating absorption of venom in the anterior chamber, and 2 were permanently blinded. Treatment of this rare emergency is discussed: immediate irrigation of the eye with water, careful examination for corneal abrasion, and prevention of secondary infection are recommended. The value of local specific antivenom is unproven."}
{"id": "PMID:1084701", "title": "Bleeding from stapler anastomosis.", "content": "Three patients with massive bleeding after stapler-performed intestinal anastomosis are reported on. Two patients required secondary operation for control. The cause was mechanical instrument failure. Periodic in vitro testing is recommended as a preventive measure.", "contents": "Bleeding from stapler anastomosis. Three patients with massive bleeding after stapler-performed intestinal anastomosis are reported on. Two patients required secondary operation for control. The cause was mechanical instrument failure. Periodic in vitro testing is recommended as a preventive measure."}
{"id": "PMID:1084702", "title": "Age-related changes in the proteolytic enzymes of mammalian lens.", "content": "A longitudinal study has been conducted on three exopeptidases which include an esterase, leucine aminopeptidase, and a triglycinopeptidase and two endopeptidases, a neutral protease and a proteinase or catheptic activity in bovine lenses. The esterase and leucine aminopeptidase behave similarily showing increased activities with aging. Triglycinopeptidase has a somewhat lower order of magnitude when compared to the other exopeptidases. The neutral protease shows initially high activity early in the developmental life period, whereas, the proteinase or catheptic activity indicates a continuous increase with aging. Data whereas, the proteinase or catheptic activity indicates a continuous increase with aging. Data is also provided on enzymic activity in the different sections of the lens which include the combined anterior cortex and epithelium, lens nucleus, posterior cortex, and equatorial ring.", "contents": "Age-related changes in the proteolytic enzymes of mammalian lens. A longitudinal study has been conducted on three exopeptidases which include an esterase, leucine aminopeptidase, and a triglycinopeptidase and two endopeptidases, a neutral protease and a proteinase or catheptic activity in bovine lenses. The esterase and leucine aminopeptidase behave similarily showing increased activities with aging. Triglycinopeptidase has a somewhat lower order of magnitude when compared to the other exopeptidases. The neutral protease shows initially high activity early in the developmental life period, whereas, the proteinase or catheptic activity indicates a continuous increase with aging. Data whereas, the proteinase or catheptic activity indicates a continuous increase with aging. Data is also provided on enzymic activity in the different sections of the lens which include the combined anterior cortex and epithelium, lens nucleus, posterior cortex, and equatorial ring."}
{"id": "PMID:1084703", "title": "EOG: light test and dark test.", "content": "The EOG test is divided into two parts: a light and a dark test. The adaptation period is extended to 30 min. During this adaptation period the luminance in the cabin decreases (or increases) slowly to the starting value of the light or dark step. In a model, we studied the influence of this ramplike luminance course on the results. Standard values for the test were calculated with 30 test persons. Our test results show a lower relative standard deviation (V = 10%) compared with earlier published results.", "contents": "EOG: light test and dark test. The EOG test is divided into two parts: a light and a dark test. The adaptation period is extended to 30 min. During this adaptation period the luminance in the cabin decreases (or increases) slowly to the starting value of the light or dark step. In a model, we studied the influence of this ramplike luminance course on the results. Standard values for the test were calculated with 30 test persons. Our test results show a lower relative standard deviation (V = 10%) compared with earlier published results."}
{"id": "PMID:1084704", "title": "Experimental Fibroplasia in the rabbit vitreous. Retinal detachment induced by autologous fibroblasts.", "content": "Fibroblasts were cultured from autologous dermal connective tissue of albino rabbits and implanted (1 X 10(6)-3 X 10(6) cells) into the vitreous space of 18 rabbit eyes under sterile surgical conditions. In 13 eyes a distinct growth of the cells occurred, and vitreous strands were formed. In 9 eyes a retinal detachment followed, and in some it was associated with traction of optic nerve tissue into the vitreous. The proliferating fibroblasts produced collagen fibrils showing a band period of 45-55 nm. Intravitreal collagen formation is considered to be a pathologic phenomenon capable of inducing the development of vitreous retraction and retinal detachment.", "contents": "Experimental Fibroplasia in the rabbit vitreous. Retinal detachment induced by autologous fibroblasts. Fibroblasts were cultured from autologous dermal connective tissue of albino rabbits and implanted (1 X 10(6)-3 X 10(6) cells) into the vitreous space of 18 rabbit eyes under sterile surgical conditions. In 13 eyes a distinct growth of the cells occurred, and vitreous strands were formed. In 9 eyes a retinal detachment followed, and in some it was associated with traction of optic nerve tissue into the vitreous. The proliferating fibroblasts produced collagen fibrils showing a band period of 45-55 nm. Intravitreal collagen formation is considered to be a pathologic phenomenon capable of inducing the development of vitreous retraction and retinal detachment."}
{"id": "PMID:1084705", "title": "[Intraocular pressure measurements on soft contact lenses (author's transl)].", "content": "The pneumotonograph was evaluated for its capability to record and measure intraocular pressure through soft contact lenses. In a series of patients with chronic corneal diseases treated with soft contact lenses, good pressure and intraocular pulsations were recorded both with and without the soft lenses. Areas of scarred cornea give incorrect values, whereas areas of corneal edema give values similar to those obtained in the presence of a soft lens and are believed to reflect accurate and reliable pressure readings. In normal eyes, it has been found that the presence of a plain soft lens does not modify the accuracy of pressure measurement. High values do occur, however, when the refraction of the lens exceeds eight diopters.", "contents": "[Intraocular pressure measurements on soft contact lenses (author's transl)]. The pneumotonograph was evaluated for its capability to record and measure intraocular pressure through soft contact lenses. In a series of patients with chronic corneal diseases treated with soft contact lenses, good pressure and intraocular pulsations were recorded both with and without the soft lenses. Areas of scarred cornea give incorrect values, whereas areas of corneal edema give values similar to those obtained in the presence of a soft lens and are believed to reflect accurate and reliable pressure readings. In normal eyes, it has been found that the presence of a plain soft lens does not modify the accuracy of pressure measurement. High values do occur, however, when the refraction of the lens exceeds eight diopters."}
{"id": "PMID:1084706", "title": "[Problems in the determination of color coordinates after the visual comparison method on the fundus of the eye (author's transl)].", "content": "A short description of color measurements on the fundus according to the method of visual comparison. The Zeiss Fundus camera equipped with an attachment served as the basic instrument. The method will not have clinical value until exact statistical values for the range of error of the different methods of measurement are available. A large number of color measurements in a short period of time on one patient's eye for gauging purposes is not possible. Therefore series of measurements are carried out on a phantom eye in order to determine the range of error in the color measurements under different conditions. Description of the results. The accuracy of the measurements which can be reached under ideal conditions with the phantom eye cannot always be reached with the patient's eye. Description of a model case for further series of measurements. The same errors of measurement appeared for reddish, yellow-orangeish, and almost white color areas. The fact that the adjustable lamp had to be replaced had practically no consequences. A series of measurements conducted according to the highest and lowest luminary densities allowed by the German Industrial Norms justifies the decision to limit the luminary density for the color measurements on the ocular fundus to 40 to 70 asb. Under the described conditions the error of measurement is smaller than is necessary clinically.", "contents": "[Problems in the determination of color coordinates after the visual comparison method on the fundus of the eye (author's transl)]. A short description of color measurements on the fundus according to the method of visual comparison. The Zeiss Fundus camera equipped with an attachment served as the basic instrument. The method will not have clinical value until exact statistical values for the range of error of the different methods of measurement are available. A large number of color measurements in a short period of time on one patient's eye for gauging purposes is not possible. Therefore series of measurements are carried out on a phantom eye in order to determine the range of error in the color measurements under different conditions. Description of the results. The accuracy of the measurements which can be reached under ideal conditions with the phantom eye cannot always be reached with the patient's eye. Description of a model case for further series of measurements. The same errors of measurement appeared for reddish, yellow-orangeish, and almost white color areas. The fact that the adjustable lamp had to be replaced had practically no consequences. A series of measurements conducted according to the highest and lowest luminary densities allowed by the German Industrial Norms justifies the decision to limit the luminary density for the color measurements on the ocular fundus to 40 to 70 asb. Under the described conditions the error of measurement is smaller than is necessary clinically."}
{"id": "PMID:1084707", "title": "Concomitant Subdural and Preretinal hemorrhage.", "content": "A case report is presented of a patient with a chronic subdural hematoma, who only once had a short episode of central nervous signs. The outstanding symptom was a seemingly spontaneous introcular hemorrhage, ipsilateral to the subdural hematoma. The mechanism of the ocular manifestation is discussed.", "contents": "Concomitant Subdural and Preretinal hemorrhage. A case report is presented of a patient with a chronic subdural hematoma, who only once had a short episode of central nervous signs. The outstanding symptom was a seemingly spontaneous introcular hemorrhage, ipsilateral to the subdural hematoma. The mechanism of the ocular manifestation is discussed."}
{"id": "PMID:1084708", "title": "Indentification and radioimmunologic estimation of sexual steroid hormones in aqueous humor and vitreous of rabbit eye.", "content": "The occurrence of testosterone, estrone, and estradiol-17 alpha in aqueous humor and vitreous body of the rabbit eye was demonstrated. The identity of the steroid hormones was confirmed by their chromatographic properties, microreactions, and radiochemically by crystallization to constant specific activity of their labeled acetates. The levels of testosterone and estrogens in rabbit male and female plasma, aqueous, and vitreous were determined by radioimmunologic assay. The concentrations of the steroid hormones in plasma are higher than in aqueous and vitreous. However, testosterone in aqueous circulates in free form and lacks the binding to sexual hormone-binding globulin as in plasma.", "contents": "Indentification and radioimmunologic estimation of sexual steroid hormones in aqueous humor and vitreous of rabbit eye. The occurrence of testosterone, estrone, and estradiol-17 alpha in aqueous humor and vitreous body of the rabbit eye was demonstrated. The identity of the steroid hormones was confirmed by their chromatographic properties, microreactions, and radiochemically by crystallization to constant specific activity of their labeled acetates. The levels of testosterone and estrogens in rabbit male and female plasma, aqueous, and vitreous were determined by radioimmunologic assay. The concentrations of the steroid hormones in plasma are higher than in aqueous and vitreous. However, testosterone in aqueous circulates in free form and lacks the binding to sexual hormone-binding globulin as in plasma."}
{"id": "PMID:1084709", "title": "[Experimental ultrasonography of the rabbit eye (author's transl)].", "content": "In 20 adult rabbits of the Gelbsilber-strain the axial depth of the anterior chamber, the axial thickness of the lens, and 7 different diameters of the vitreous chamber were measured by means of bioechometry for standardization of the to date neglected experimental ultrasonography of animal eyes. For proper interpretation of echograms of the rabbit eye the particular anatomy and topography of the lens must be considered. Penetration of the large and almost spherical lens by the beam can only be avoided if the probe is placed behind the limbus or behind the equator of the globe. In the axial ultrasonogram the high single peaked echo of the posterior lens capsule appears between the third and fourth fifth of the entire axial length. In all other types of echogram the beam passes through peripheral sections of the lens oblique, and produces several small echoes, thus eventually simulating a pathologic echogram of the vitreous body.", "contents": "[Experimental ultrasonography of the rabbit eye (author's transl)]. In 20 adult rabbits of the Gelbsilber-strain the axial depth of the anterior chamber, the axial thickness of the lens, and 7 different diameters of the vitreous chamber were measured by means of bioechometry for standardization of the to date neglected experimental ultrasonography of animal eyes. For proper interpretation of echograms of the rabbit eye the particular anatomy and topography of the lens must be considered. Penetration of the large and almost spherical lens by the beam can only be avoided if the probe is placed behind the limbus or behind the equator of the globe. In the axial ultrasonogram the high single peaked echo of the posterior lens capsule appears between the third and fourth fifth of the entire axial length. In all other types of echogram the beam passes through peripheral sections of the lens oblique, and produces several small echoes, thus eventually simulating a pathologic echogram of the vitreous body."}
{"id": "PMID:1084710", "title": "Antibiotic prophylaxis in penetrating injuries of the eye.", "content": "In a specially developed experimental model of an infected penetrating eye injury the importance of time and mode of antibiotic administration in the prophylaxis of post-traumatic eye infection was evaluated. It was concluded that the early administration of an antibiotic to which infecting organisms are sensitive can prevent the development of an inflammatory reaction in the majority of injured eyes. The late administration of the same antibiotic (6 h after injury) has no effect at all. Subconjunctival injection was found to be superior to intramuscular if the antibiotic was given at a similar time after injury. The results of this experiment stress the importance of very early antibiotic administration in the prophylaxis of an infection in penetrating eye injuries.", "contents": "Antibiotic prophylaxis in penetrating injuries of the eye. In a specially developed experimental model of an infected penetrating eye injury the importance of time and mode of antibiotic administration in the prophylaxis of post-traumatic eye infection was evaluated. It was concluded that the early administration of an antibiotic to which infecting organisms are sensitive can prevent the development of an inflammatory reaction in the majority of injured eyes. The late administration of the same antibiotic (6 h after injury) has no effect at all. Subconjunctival injection was found to be superior to intramuscular if the antibiotic was given at a similar time after injury. The results of this experiment stress the importance of very early antibiotic administration in the prophylaxis of an infection in penetrating eye injuries."}
{"id": "PMID:1084711", "title": "[Contribution on the infraocular optics (author's transl)].", "content": "Experience indicates that a person will have a comfortable vision only if the sizes of the image in both eyes are approximately equal; a tolerance of approximately 5% is acceptable for this. In other words both optical systems, consisting of a spectacle lens, a contact lens, and the eye must have approximately the same focal length. Patients who have one phacic and one aphacic eye will usually possess quite different focal lengths with conventional contact lens correction. However, with the addition of spectacles and of appropriate contact lenses on one or both eyes, the ophthalmologist has at his disposal a new variable. The given formulas and rules of thumb enable him to choose the strength of spectacles-contact lens combinations so that the patient will also have sharp vision in both eyes and regain a comfortable binocular sight. The method is illustrated by examples.", "contents": "[Contribution on the infraocular optics (author's transl)]. Experience indicates that a person will have a comfortable vision only if the sizes of the image in both eyes are approximately equal; a tolerance of approximately 5% is acceptable for this. In other words both optical systems, consisting of a spectacle lens, a contact lens, and the eye must have approximately the same focal length. Patients who have one phacic and one aphacic eye will usually possess quite different focal lengths with conventional contact lens correction. However, with the addition of spectacles and of appropriate contact lenses on one or both eyes, the ophthalmologist has at his disposal a new variable. The given formulas and rules of thumb enable him to choose the strength of spectacles-contact lens combinations so that the patient will also have sharp vision in both eyes and regain a comfortable binocular sight. The method is illustrated by examples."}
{"id": "PMID:1084712", "title": "[On the ultrastructure of proliferative changes (cell metaplasia) in the retinal pigment epithelium over malignant melanomas of the choroid (author's transl)].", "content": "Proliferations of the retinal pigment epithelium over malignant melanomas of the choroid from three human eyes were examined at various locations: 1. at the posterior pole, 2. in the equator region, 3. at the ora serrata. The observation was made that in each proliferation one cell type dominates which could be distinguished by its shape and cytoplasm from those of the other proliferations. Only the cells of the proliferation at the posterior pole correspond with the normal pigment epithelium. Most of the cells in the equator region show several properties of glia cells whereas these of the ora resemble fibroblasts. Our findings suggest that the proliferated pigment epithelium over malignant melanomas of the choroid is capable of undergoing metaplastic changes.", "contents": "[On the ultrastructure of proliferative changes (cell metaplasia) in the retinal pigment epithelium over malignant melanomas of the choroid (author's transl)]. Proliferations of the retinal pigment epithelium over malignant melanomas of the choroid from three human eyes were examined at various locations: 1. at the posterior pole, 2. in the equator region, 3. at the ora serrata. The observation was made that in each proliferation one cell type dominates which could be distinguished by its shape and cytoplasm from those of the other proliferations. Only the cells of the proliferation at the posterior pole correspond with the normal pigment epithelium. Most of the cells in the equator region show several properties of glia cells whereas these of the ora resemble fibroblasts. Our findings suggest that the proliferated pigment epithelium over malignant melanomas of the choroid is capable of undergoing metaplastic changes."}
{"id": "PMID:1084713", "title": "Glucocorticoids and intraocular pressure: an experimental study.", "content": "The state of intraocular pressure and aqueous humor dynamics as well as histomorphologic and histochemical change of the eye tissues as a result of administration of glucocorticoids were studied in 62 rabbits. After administration of glucocorticoids by instillations and subconjunctival injections there was a trend toward increased intraocular pressure but no statistically significant change in the intraocular pressure was demonstrated. When glucocorticoids were administered for 1 month, a significant increase in production and outflow of aqueous humor was established. Continuation of the experiments for 2 months or longer was accompanied by a significant decrease of the outflow facility coefficient and inhibition of aqueous humor formation. Histologic and histochemical examinations of the eyes of the rabbits studied for 2 months revealed marked dystrophic changes in the trabecular meshwork tissues.", "contents": "Glucocorticoids and intraocular pressure: an experimental study. The state of intraocular pressure and aqueous humor dynamics as well as histomorphologic and histochemical change of the eye tissues as a result of administration of glucocorticoids were studied in 62 rabbits. After administration of glucocorticoids by instillations and subconjunctival injections there was a trend toward increased intraocular pressure but no statistically significant change in the intraocular pressure was demonstrated. When glucocorticoids were administered for 1 month, a significant increase in production and outflow of aqueous humor was established. Continuation of the experiments for 2 months or longer was accompanied by a significant decrease of the outflow facility coefficient and inhibition of aqueous humor formation. Histologic and histochemical examinations of the eyes of the rabbits studied for 2 months revealed marked dystrophic changes in the trabecular meshwork tissues."}
{"id": "PMID:1084715", "title": "T and B lymphocyte rosettes in bronchial asthma.", "content": "In this study the percentage of T and B lymphocytes was not significantly different between asthmatics and normal subjects. However, the absolute number of T and B lymphocytes was lower in asthmatics, which supports the hypothesis that atopy may be associated with immunodeficiency state.", "contents": "T and B lymphocyte rosettes in bronchial asthma. In this study the percentage of T and B lymphocytes was not significantly different between asthmatics and normal subjects. However, the absolute number of T and B lymphocytes was lower in asthmatics, which supports the hypothesis that atopy may be associated with immunodeficiency state."}
{"id": "PMID:1084716", "title": "Increase in E-rosettes after transfer factor (TF) treatment: fractionation of TF.", "content": "The effect of transfer factor (TF) on E-rosettes (ER) was studied in vivo in a patient with Hodgkin's disease. Transfer factor was given in doses of 10 units/sq m intramuscularly. The ER forming cells and the ER scores were determined. The ER score method took into account the number of erythrocytes in each rosette. The increase in ER score was maximum at 24 hours and it declined during the following one to two weeks. It was suggested that TF may have to be given more frequently than indicated by the persistence of skin reactivity. TF was fractionated with high pressure liquid chromatography. Fraction 2 increased ER in a patient with Schwannoma. Non-specificity of TF was also discussed.", "contents": "Increase in E-rosettes after transfer factor (TF) treatment: fractionation of TF. The effect of transfer factor (TF) on E-rosettes (ER) was studied in vivo in a patient with Hodgkin's disease. Transfer factor was given in doses of 10 units/sq m intramuscularly. The ER forming cells and the ER scores were determined. The ER score method took into account the number of erythrocytes in each rosette. The increase in ER score was maximum at 24 hours and it declined during the following one to two weeks. It was suggested that TF may have to be given more frequently than indicated by the persistence of skin reactivity. TF was fractionated with high pressure liquid chromatography. Fraction 2 increased ER in a patient with Schwannoma. Non-specificity of TF was also discussed."}
{"id": "PMID:1084718", "title": "Follow-up of patients with saphenous vein bypass grafts for angina pectoris.", "content": "These studies are in agreement with others that aortocoronary vein grafts done for severe angina pectoris are associated with a low operative and late mortality rate. Most patients were significantly improved in regard to pain and exercise tolerance. \"Optimal\" graft site is associated with a graft patency rate of about 90 per cent. There is clear evidence that left ventricular function, particularly with exercise, is improved in some patients. When a graft is placed in an obstructed coronary artery supplying a segment of abnormally contracting myocardium, there frequently is significant improvement of contraction when the graft remains patent.", "contents": "Follow-up of patients with saphenous vein bypass grafts for angina pectoris. These studies are in agreement with others that aortocoronary vein grafts done for severe angina pectoris are associated with a low operative and late mortality rate. Most patients were significantly improved in regard to pain and exercise tolerance. \"Optimal\" graft site is associated with a graft patency rate of about 90 per cent. There is clear evidence that left ventricular function, particularly with exercise, is improved in some patients. When a graft is placed in an obstructed coronary artery supplying a segment of abnormally contracting myocardium, there frequently is significant improvement of contraction when the graft remains patent."}
{"id": "PMID:1084719", "title": "Ruptured submucosal gastric artery microaneurysm.", "content": "A patient with massive upper gastrointestinal hemorrhage from a ruptured submucosal gastric artery microaneurysm is described. Intraoperative diagnosis was made and wedge resection of the lesion resulted in survival of the patient. Forty-two cases in this entity have been reported in the literature, with nonoperative therapy being uniformly fatal; there were only four cases of successful surgical management. An increased awareness of this entity in cases of unexplained gastrointestinal hemorrhage is the key to diagnosis and successful management.", "contents": "Ruptured submucosal gastric artery microaneurysm. A patient with massive upper gastrointestinal hemorrhage from a ruptured submucosal gastric artery microaneurysm is described. Intraoperative diagnosis was made and wedge resection of the lesion resulted in survival of the patient. Forty-two cases in this entity have been reported in the literature, with nonoperative therapy being uniformly fatal; there were only four cases of successful surgical management. An increased awareness of this entity in cases of unexplained gastrointestinal hemorrhage is the key to diagnosis and successful management."}
{"id": "PMID:1084722", "title": "Alpha1-antitrypsin deficiency and pancreatic fibrosis.", "content": "A 39-year-old woman with homozygous ZZ alpha1-antitrypsin deficiency was found to have pancreatic fibrosis in addition to the recognized pulmonary and hepatic abnormalities. Pancreatic exocrine function was normal but endocrine studies showed glucose intolerance. Pancreatic fibrosis may be another manifestation of alpha1-antitrypsin deficiency.", "contents": "Alpha1-antitrypsin deficiency and pancreatic fibrosis. A 39-year-old woman with homozygous ZZ alpha1-antitrypsin deficiency was found to have pancreatic fibrosis in addition to the recognized pulmonary and hepatic abnormalities. Pancreatic exocrine function was normal but endocrine studies showed glucose intolerance. Pancreatic fibrosis may be another manifestation of alpha1-antitrypsin deficiency."}
{"id": "PMID:1084723", "title": "Coronary artery surgery: the use of decision analysis.", "content": "The choice between coronary by-pass surgery and medical therapy in patients with angiographically documented coronary artery disease was examined. This decision analysis included consideration of patient preferences, severity of disease, prognosis with medical therapy, surgical mortality rate, graft patency rate, the probability that surgery will provide both short-term and long-term pain relief, and the probability that surgery will alter long-term survival. Coronary surgery was found to be the preferred therapy in many patients with disabling angina; it was rarely found to be the preferred therapy in asymptomatic patients, even those with proximal obstruction of the left anterior descending coronary artery. The therapeutic decision was strongly affected by differences in patient attitudes and differences in the past results of the prospective surgeon. This paper presents both a data base and a methodology that allow the physician to apply decision analysis to individual patients with coronary artery disease.", "contents": "Coronary artery surgery: the use of decision analysis. The choice between coronary by-pass surgery and medical therapy in patients with angiographically documented coronary artery disease was examined. This decision analysis included consideration of patient preferences, severity of disease, prognosis with medical therapy, surgical mortality rate, graft patency rate, the probability that surgery will provide both short-term and long-term pain relief, and the probability that surgery will alter long-term survival. Coronary surgery was found to be the preferred therapy in many patients with disabling angina; it was rarely found to be the preferred therapy in asymptomatic patients, even those with proximal obstruction of the left anterior descending coronary artery. The therapeutic decision was strongly affected by differences in patient attitudes and differences in the past results of the prospective surgeon. This paper presents both a data base and a methodology that allow the physician to apply decision analysis to individual patients with coronary artery disease."}
{"id": "PMID:1084724", "title": "Intestinal bypass operation as a treatment for obesity.", "content": "The intestinal bypass operation has come into wide use as a method for treating the grossly obese patient. Because it carries risks of morbidity and mortality, it is only suitable for those who meet certain minimal criteria. During the first year after surgery, weight loss varies from 9.1 to 68.0 kg [20 to 150 lb]. These patients have shown improved self-esteem and a reduction in the amount of depression. Mortality for this operation is approximately 4%; many serious complications (liver failure, pulmonary embolus, improper wound healing, severe loss of minerals, and vitamin deficiencies) occur. Hyperoxaluria may result from increased intestinal absorption. A dilated colon (pseudocolonic obstruction) may develop from bacterial overgrowth in the distal segment of the bypassed intestine. Since these serious complications affect 40% of the patients, the operation should be done by trained and skilled physicians who will also be able to manage the long-term postoperative period.", "contents": "Intestinal bypass operation as a treatment for obesity. The intestinal bypass operation has come into wide use as a method for treating the grossly obese patient. Because it carries risks of morbidity and mortality, it is only suitable for those who meet certain minimal criteria. During the first year after surgery, weight loss varies from 9.1 to 68.0 kg [20 to 150 lb]. These patients have shown improved self-esteem and a reduction in the amount of depression. Mortality for this operation is approximately 4%; many serious complications (liver failure, pulmonary embolus, improper wound healing, severe loss of minerals, and vitamin deficiencies) occur. Hyperoxaluria may result from increased intestinal absorption. A dilated colon (pseudocolonic obstruction) may develop from bacterial overgrowth in the distal segment of the bypassed intestine. Since these serious complications affect 40% of the patients, the operation should be done by trained and skilled physicians who will also be able to manage the long-term postoperative period."}
{"id": "PMID:1084727", "title": "Characterization of an ampicillin-resistant Haemophilus influenzae type B.", "content": "A 28-year-old female in Denver was found in early 1974 to have frontal sinusitis, osteomyelitis, and bacteremia due to Haemophilus influenzae, type B. The minimal inhibitory concentration of ampicillin for this organism was 100 mug/ml and the minimal bactericidal concentration was >100 mug/ml. It was inhibited by chloramphenicol at 0.4 mug/ml. Further studies demonstrated that ampicillin and methicillin were synergistic against this organism. It was shown to produce a diffusible beta-lactamase. Transferase of resistance from this organism to a susceptible Haemophilus parainfluenzae and a reciprocal transfer were accomplished. A test for transformation was negative as was a test for reversal of resistance by ethylenediaminetetraacetic acid.", "contents": "Characterization of an ampicillin-resistant Haemophilus influenzae type B. A 28-year-old female in Denver was found in early 1974 to have frontal sinusitis, osteomyelitis, and bacteremia due to Haemophilus influenzae, type B. The minimal inhibitory concentration of ampicillin for this organism was 100 mug/ml and the minimal bactericidal concentration was >100 mug/ml. It was inhibited by chloramphenicol at 0.4 mug/ml. Further studies demonstrated that ampicillin and methicillin were synergistic against this organism. It was shown to produce a diffusible beta-lactamase. Transferase of resistance from this organism to a susceptible Haemophilus parainfluenzae and a reciprocal transfer were accomplished. A test for transformation was negative as was a test for reversal of resistance by ethylenediaminetetraacetic acid."}
{"id": "PMID:1084725", "title": "[Aortocoronary bypass].", "content": "Surgical revascularisation of the myocardium by aorto-coronary by-pass, has become in recent years, the operation of choice for the treatment of lesions of coronary arteriosclerosis. The quality of the results observed in our series, of 200 operated patients, and those reported by other authors, is emphasized. The problems raised by extension of this surgery, especially in the precise pre-operative assessment of ventricular function, the optimum material to use for realisatin of the graft and the course of coronary lesions after by-pass operations are then discussed.", "contents": "[Aortocoronary bypass]. Surgical revascularisation of the myocardium by aorto-coronary by-pass, has become in recent years, the operation of choice for the treatment of lesions of coronary arteriosclerosis. The quality of the results observed in our series, of 200 operated patients, and those reported by other authors, is emphasized. The problems raised by extension of this surgery, especially in the precise pre-operative assessment of ventricular function, the optimum material to use for realisatin of the graft and the course of coronary lesions after by-pass operations are then discussed."}
{"id": "PMID:1084726", "title": "[Influence of injection of cortisone acetate on blast transformation and antibody-dependent cell-mediated cytotoxicity in rabbit (author's transl)].", "content": "Inhibition of circulating antibody production by steroids has been repeatedly shown in steroid-sensitive animals such as mice or rabbits. It is well known that after a single injection of 5 mg of cortisone acetate, mouse spleen cells loose their ability to be transformed by B cell mitogens such as lipopolysaccharides or Nocardia water soluble mitogen. Therefore it was of interest to study in the rabbit the influence of cortisone on the reactivity of spleen cells to a B cell mitogen (blast transformation and polyclonal strimulation) and their ability to be cytotoxic in the antibody-dependent cell-mediated cytotoxicity assay. Our data demonstrate that the cells which mediate such a cytotoxicity are cortisone-resistant whereas the B lymphocytes--which can be transformed and polyclonally activated by these mitogens--are sensitive to cortisone treatment.", "contents": "[Influence of injection of cortisone acetate on blast transformation and antibody-dependent cell-mediated cytotoxicity in rabbit (author's transl)]. Inhibition of circulating antibody production by steroids has been repeatedly shown in steroid-sensitive animals such as mice or rabbits. It is well known that after a single injection of 5 mg of cortisone acetate, mouse spleen cells loose their ability to be transformed by B cell mitogens such as lipopolysaccharides or Nocardia water soluble mitogen. Therefore it was of interest to study in the rabbit the influence of cortisone on the reactivity of spleen cells to a B cell mitogen (blast transformation and polyclonal strimulation) and their ability to be cytotoxic in the antibody-dependent cell-mediated cytotoxicity assay. Our data demonstrate that the cells which mediate such a cytotoxicity are cortisone-resistant whereas the B lymphocytes--which can be transformed and polyclonally activated by these mitogens--are sensitive to cortisone treatment."}
{"id": "PMID:1084732", "title": "Aortocoronary saphenous vein autograft accidentally attached to a coronary vein: follow-up angiography and surgical correction of the resultant arteriovenous fistula.", "content": "The fate of aortocoronary saphenous vein bypass graft to the left anterior descending (LAD) coronary vein is reported. The vein graft communicated with the coronary sinus through the proximal LAD vein, producing a functional coronary arteriovenous fistula. The LAD vein was totally occluded distally at follow-up four months after operation. The natural history of congenital fistulas between coronary arteries and the coronary sinus suggested that bacterial endocarditis, pulmonary hypertension, and cardiac failure were all possible future complications in this patient. Operation was performed to revascularize the LAD artery to relieve persistent angina, and to close the fistula. Postoperative angiography showed a patent graft to the LAD artery with complete obliteration of the fistula. The patient is asymptomatic ten months after operation.", "contents": "Aortocoronary saphenous vein autograft accidentally attached to a coronary vein: follow-up angiography and surgical correction of the resultant arteriovenous fistula. The fate of aortocoronary saphenous vein bypass graft to the left anterior descending (LAD) coronary vein is reported. The vein graft communicated with the coronary sinus through the proximal LAD vein, producing a functional coronary arteriovenous fistula. The LAD vein was totally occluded distally at follow-up four months after operation. The natural history of congenital fistulas between coronary arteries and the coronary sinus suggested that bacterial endocarditis, pulmonary hypertension, and cardiac failure were all possible future complications in this patient. Operation was performed to revascularize the LAD artery to relieve persistent angina, and to close the fistula. Postoperative angiography showed a patent graft to the LAD artery with complete obliteration of the fistula. The patient is asymptomatic ten months after operation."}
{"id": "PMID:1084734", "title": "Gait analysis in stroke patients to assess treatments of foot-drop.", "content": "By using the combined, computerized analysis of stroboscopic photography, pattern recognition devices, electromyography and foot switch outputs, gait patterns were studied in 15 stroke patients with foot-drop. Three patients were treated with a peroneal nerve stimulator for five weeks; six received intensive physical therapy; and six received both physical therapy and biofeedback training three times a week for five weeks. Each patient was examined at initial visit and after five weeks. Based upon case evaluations, both the patients with the stimulator treatment and those with biofeedback training showed and maintained improvement of gait pattern following the treatment period. This result suggests that the biofeedback and peroneal-stimulator technique may have a common mechanism that should be exploited in the rehabilitation of stroke patients.", "contents": "Gait analysis in stroke patients to assess treatments of foot-drop. By using the combined, computerized analysis of stroboscopic photography, pattern recognition devices, electromyography and foot switch outputs, gait patterns were studied in 15 stroke patients with foot-drop. Three patients were treated with a peroneal nerve stimulator for five weeks; six received intensive physical therapy; and six received both physical therapy and biofeedback training three times a week for five weeks. Each patient was examined at initial visit and after five weeks. Based upon case evaluations, both the patients with the stimulator treatment and those with biofeedback training showed and maintained improvement of gait pattern following the treatment period. This result suggests that the biofeedback and peroneal-stimulator technique may have a common mechanism that should be exploited in the rehabilitation of stroke patients."}
{"id": "PMID:1084735", "title": "Electrically induced flexion reflex in gait training of hemiplegic patients: induction of the reflex.", "content": "Since the introduction of the concept of functional electrical stimulation, the use of multiple stimulation sites to activate several paretic muscles in a coordinated fashion during ambulation has been suggested. Rather than stimulating multiple muscles, we have applied a train of electrical pulses to a single skin area to activate several muscles simultaneously through the induction of a flexion reflex, and evaluated the effectiveness of this reflex in producing or assisting the swing phase of the gait cycle in the hemiplegic patient. Two considerations in selecting the best stimulus site were (1) the magnitude of the reflex as measured by the length of time the foot was off the floor following a stimulus and (2) the patient's tolerance to the stimulus. The magnitude of the reflex was proportional to the strength of the stimulus current in all skin areas tested; namely the sole, dorsum of the foot, and lower posterior thigh. Of the six patients tested a flexion reflex was not elicitable by sole stimulation in one and by posterior thigh stimulation in another. In every case, compared to the two other areas, stimulation of the sole was best tolerated and produced responses of equal or greater magnitude with the same intensity of current, even though larger voltages were required owing to greater tissue impedances. With a fixed frequency of 30 Hz, a pulse duration of 3 msec, and a stimulus current sufficient to produce the reflex, the duration of the reflex could be adjusted for each patient by varying the train duration from 100 to 700 msec. This technique offers a means of providing or assisting the swing phase in the gait training of the hemiplegic patient.", "contents": "Electrically induced flexion reflex in gait training of hemiplegic patients: induction of the reflex. Since the introduction of the concept of functional electrical stimulation, the use of multiple stimulation sites to activate several paretic muscles in a coordinated fashion during ambulation has been suggested. Rather than stimulating multiple muscles, we have applied a train of electrical pulses to a single skin area to activate several muscles simultaneously through the induction of a flexion reflex, and evaluated the effectiveness of this reflex in producing or assisting the swing phase of the gait cycle in the hemiplegic patient. Two considerations in selecting the best stimulus site were (1) the magnitude of the reflex as measured by the length of time the foot was off the floor following a stimulus and (2) the patient's tolerance to the stimulus. The magnitude of the reflex was proportional to the strength of the stimulus current in all skin areas tested; namely the sole, dorsum of the foot, and lower posterior thigh. Of the six patients tested a flexion reflex was not elicitable by sole stimulation in one and by posterior thigh stimulation in another. In every case, compared to the two other areas, stimulation of the sole was best tolerated and produced responses of equal or greater magnitude with the same intensity of current, even though larger voltages were required owing to greater tissue impedances. With a fixed frequency of 30 Hz, a pulse duration of 3 msec, and a stimulus current sufficient to produce the reflex, the duration of the reflex could be adjusted for each patient by varying the train duration from 100 to 700 msec. This technique offers a means of providing or assisting the swing phase in the gait training of the hemiplegic patient."}
{"id": "PMID:1084731", "title": "[Research on V. cholerae anti-antibodies (agglutinating and vibriocidal) in patients affected by cholera-like gastroenteritis. Studies performed during the cholera epidemic occurred in Apulia in August-September 1973].", "content": "Agglutinating and vibriocidal antibody titers anti-V. cholerae of the serotypes Ogawa and Inaba, were determined in sera from 189 patients with cholera-like diarrheas during the epidemic of cholera occurred in August-September 1973, in Bari. Antibodies were determined in 74,2% of 70 patients, whose in the faeces and vomiting were isolated strains identified as V. cholerae, biotype El Tor, serotype Ogawa. This frequency was higher against the serotype Ogawa. The antibodies could be proved very early, 4-6 days from the beginning of symptomatology, but they disappeared as rapidly; really, at 2.3 months antibodies were determined in 7,7% only of the above mentioned subjects. As regards the other 119 patients with non-vibrio, cholera-like diarrheas, antibodies were determined in 28 subjects only; 27 of these has received cholera-vaccine some weeks before. One single case remained which was bacteriological negative, but showed signifcant agglutinating and vibriocidal antibody titers against the serotypes Ogawa and Inaba.", "contents": "[Research on V. cholerae anti-antibodies (agglutinating and vibriocidal) in patients affected by cholera-like gastroenteritis. Studies performed during the cholera epidemic occurred in Apulia in August-September 1973]. Agglutinating and vibriocidal antibody titers anti-V. cholerae of the serotypes Ogawa and Inaba, were determined in sera from 189 patients with cholera-like diarrheas during the epidemic of cholera occurred in August-September 1973, in Bari. Antibodies were determined in 74,2% of 70 patients, whose in the faeces and vomiting were isolated strains identified as V. cholerae, biotype El Tor, serotype Ogawa. This frequency was higher against the serotype Ogawa. The antibodies could be proved very early, 4-6 days from the beginning of symptomatology, but they disappeared as rapidly; really, at 2.3 months antibodies were determined in 7,7% only of the above mentioned subjects. As regards the other 119 patients with non-vibrio, cholera-like diarrheas, antibodies were determined in 28 subjects only; 27 of these has received cholera-vaccine some weeks before. One single case remained which was bacteriological negative, but showed signifcant agglutinating and vibriocidal antibody titers against the serotypes Ogawa and Inaba."}
{"id": "PMID:1084737", "title": "Endoscopic argon-ion laser phototherapy of bleeding gastric lesions.", "content": "An argon-ion laser, coupled to a flexible fiberoptic endoscope, was used to photocoagulate experimentally produced bleeding gastric ulcers in dogs. Argon-ion laser phototherapy rapidly and effectively achieved hemostasis in all bleeding ulcers. Bleeding arteries up to 2 mm in diameter were photocoagulated. Intact normal gastric mucosa was exposed to laser energy for varying periods of time to determine if transmural photothermal injury occurred. Exposure for greater than 15 seconds at 1.0 w of throughput power caused microscopical evidence of transmural injury. Healed, laser-treated ulcers could not be differentiated from nontreated control ulcers or normal stomach one month after injury. The Beer law and the heattransfer factors relating to soft tissues are discussed. The coupled argon-ion laser-endoscope may be a safe tool to treat self-limited superficial bleeding gastric lesions.", "contents": "Endoscopic argon-ion laser phototherapy of bleeding gastric lesions. An argon-ion laser, coupled to a flexible fiberoptic endoscope, was used to photocoagulate experimentally produced bleeding gastric ulcers in dogs. Argon-ion laser phototherapy rapidly and effectively achieved hemostasis in all bleeding ulcers. Bleeding arteries up to 2 mm in diameter were photocoagulated. Intact normal gastric mucosa was exposed to laser energy for varying periods of time to determine if transmural photothermal injury occurred. Exposure for greater than 15 seconds at 1.0 w of throughput power caused microscopical evidence of transmural injury. Healed, laser-treated ulcers could not be differentiated from nontreated control ulcers or normal stomach one month after injury. The Beer law and the heattransfer factors relating to soft tissues are discussed. The coupled argon-ion laser-endoscope may be a safe tool to treat self-limited superficial bleeding gastric lesions."}
{"id": "PMID:1084738", "title": "Subjective multivariable analysis bu computer for evaluation of coronary artery bypass.", "content": "Subjective multivariable analysis by computer was utilized to evaluate the results in a series of 659 patients undergoing coronary artery saphenous vein bypass surgery. A video terminal gave access to a remote computer. The postoperative subjective response was evaluated by questionnaires sent to patients at 6- to 12-month intervals, and was augmented by treadmill testing and postoperative coronary arteriography. Operative risk and subjective response are related only to the degree of ventricular function lost prior to surgery. Long-term  results were best in patients in whom complete revascularization was possible. Current operative risk is less than 1%; a five-year survival rate of 90% to 95% is predicted. More than 90% of patients were asymptomatic or \"greatly improved.\" Postoperative treadmill tests were negative in 88% of patients in these groups. In patients who were only \"slightly improved\" or \"not improved,\" repeat catherization showed a graft patency rate of 84%, suggesting that recurrent symptoms were related to the extent of preoperative disease or to the progression of disease in nongrafted vessels, rather than to graft occlusion.", "contents": "Subjective multivariable analysis bu computer for evaluation of coronary artery bypass. Subjective multivariable analysis by computer was utilized to evaluate the results in a series of 659 patients undergoing coronary artery saphenous vein bypass surgery. A video terminal gave access to a remote computer. The postoperative subjective response was evaluated by questionnaires sent to patients at 6- to 12-month intervals, and was augmented by treadmill testing and postoperative coronary arteriography. Operative risk and subjective response are related only to the degree of ventricular function lost prior to surgery. Long-term  results were best in patients in whom complete revascularization was possible. Current operative risk is less than 1%; a five-year survival rate of 90% to 95% is predicted. More than 90% of patients were asymptomatic or \"greatly improved.\" Postoperative treadmill tests were negative in 88% of patients in these groups. In patients who were only \"slightly improved\" or \"not improved,\" repeat catherization showed a graft patency rate of 84%, suggesting that recurrent symptoms were related to the extent of preoperative disease or to the progression of disease in nongrafted vessels, rather than to graft occlusion."}
{"id": "PMID:1084739", "title": "Operative risk in patients with previous coronary artery bypass.", "content": "The records of 141 patients who had had coronary artery bypass and myocardial revascularization were reviewed. Fifteen percent (20) of the patients in this series required a surgical procedure from three months to five years following coronary artery bypass. Twelve percent (16) of these patients had elective operations, and 3% had emergency operations. In the elective group there were no deaths. One patient had a proved myocardial infarction, and three patients had transient arrhythmias with no changes in myocardial enzymes. In the emergency group there was one death, from sepsis following splenectomy for splenic abscess. Although the series is small, the data suggest that patients with coronary artery disease who have had myocardial revascularization are acceptable risks for elective and emergency operations. Whether the risk is lower in this group as compared to that in other patients with coronary disease who have not had bypass surgery has not been demonstrated.", "contents": "Operative risk in patients with previous coronary artery bypass. The records of 141 patients who had had coronary artery bypass and myocardial revascularization were reviewed. Fifteen percent (20) of the patients in this series required a surgical procedure from three months to five years following coronary artery bypass. Twelve percent (16) of these patients had elective operations, and 3% had emergency operations. In the elective group there were no deaths. One patient had a proved myocardial infarction, and three patients had transient arrhythmias with no changes in myocardial enzymes. In the emergency group there was one death, from sepsis following splenectomy for splenic abscess. Although the series is small, the data suggest that patients with coronary artery disease who have had myocardial revascularization are acceptable risks for elective and emergency operations. Whether the risk is lower in this group as compared to that in other patients with coronary disease who have not had bypass surgery has not been demonstrated."}
{"id": "PMID:1084736", "title": "[Some biological aspects of cancer. Approach to a general theory of cancerogenesis].", "content": "Cancer is considered to be the consequence of internal evolutionary drives typical of species with open evolution (e.g. vertebrates) deficient in the primary teleonomic mechanisms underlying the maintenance of reproductive invariability. With this in mind, a biological test is hypothesized which would make it possible to identify and isolate substances from definitely non-variant species with the purpose of studying their influence on the morphogenetic processes that, at cytoplasmatic level, bring about the transformation of normal into neoplastic cells. On this basis it might be possible to establish an inhibition therapy to back up immunitary therapy as a complement to surgery and radiation.", "contents": "[Some biological aspects of cancer. Approach to a general theory of cancerogenesis]. Cancer is considered to be the consequence of internal evolutionary drives typical of species with open evolution (e.g. vertebrates) deficient in the primary teleonomic mechanisms underlying the maintenance of reproductive invariability. With this in mind, a biological test is hypothesized which would make it possible to identify and isolate substances from definitely non-variant species with the purpose of studying their influence on the morphogenetic processes that, at cytoplasmatic level, bring about the transformation of normal into neoplastic cells. On this basis it might be possible to establish an inhibition therapy to back up immunitary therapy as a complement to surgery and radiation."}
{"id": "PMID:1084741", "title": "Cerebellar-pontine angle tumors. Results of quantitative vestibulo-ocular testing.", "content": "A new quantitative vestibulo-ocular test battery was evaluated in 12 patients with surgically confirmed cerebellar-pontine angle tumors. The battery consisted of precise vestibular and visual stimuli and digital computer analyses of responses. Eleven of 12 patients, including all patients with acoustic neurolemmomas, had significant vestibular deficits on the tumor side (P less than .01). Contrary to previous reports, rotatory tests (impulse and sinusoidal acceleration) reliably identified the side and magnitude of vestibular deficit. Eight of nine patients with surgically confirmed brain stem compression had significantly impaired smooth pursuit (P less than .05). Optokinetic nystagmus was impaired in five of nine patients with brain stem compression (P less than .05). By combining the results of the entire test battery with standard clinical evaluation, a good prediction of tumor size, location, and type was possible.", "contents": "Cerebellar-pontine angle tumors. Results of quantitative vestibulo-ocular testing. A new quantitative vestibulo-ocular test battery was evaluated in 12 patients with surgically confirmed cerebellar-pontine angle tumors. The battery consisted of precise vestibular and visual stimuli and digital computer analyses of responses. Eleven of 12 patients, including all patients with acoustic neurolemmomas, had significant vestibular deficits on the tumor side (P less than .01). Contrary to previous reports, rotatory tests (impulse and sinusoidal acceleration) reliably identified the side and magnitude of vestibular deficit. Eight of nine patients with surgically confirmed brain stem compression had significantly impaired smooth pursuit (P less than .05). Optokinetic nystagmus was impaired in five of nine patients with brain stem compression (P less than .05). By combining the results of the entire test battery with standard clinical evaluation, a good prediction of tumor size, location, and type was possible."}
{"id": "PMID:1084743", "title": "Influence of immunosuppression on antinuclear antibody and survival of wehi mice.", "content": "The effect of immunosuppression with cyclophosphamide was assessed in 200 male and female Walter and Eliza Hall Institute outbred mice which are known to develop antinuclear antibody (ANA). Their longevity and the incidence of ANA were studied in comparison with controls. In females, life-span was longer despite a higher age-related incidence of ANA. Cyclophosphamide significantly delayed the development of ANA in both sexes; despite this, life-span was decreased. This is in contrast to what occurs in the highly autoimmune NZB/NZW F1 hybrid mouse. Thus, in weakly autoimmune mice the toxic effects of cyclophosphamide outweighed any benefit resulting from suppression of antinuclear autoantibodies.", "contents": "Influence of immunosuppression on antinuclear antibody and survival of wehi mice. The effect of immunosuppression with cyclophosphamide was assessed in 200 male and female Walter and Eliza Hall Institute outbred mice which are known to develop antinuclear antibody (ANA). Their longevity and the incidence of ANA were studied in comparison with controls. In females, life-span was longer despite a higher age-related incidence of ANA. Cyclophosphamide significantly delayed the development of ANA in both sexes; despite this, life-span was decreased. This is in contrast to what occurs in the highly autoimmune NZB/NZW F1 hybrid mouse. Thus, in weakly autoimmune mice the toxic effects of cyclophosphamide outweighed any benefit resulting from suppression of antinuclear autoantibodies."}
{"id": "PMID:1084744", "title": "Characterization of the normal lymphocyte population cytolytic to Burkitt's lymphoma cells of the EB2 cell line.", "content": "The normal human lymphocyte population which exhibits \"spontaneous\" cytolysis of EB2 Burkitt's lymphoma cells has been characterized. The effector cell has EA and EAC' receptors but lacks E receptors and probably surface Ig. \"Spontaneous\" anti-EB2 cytotoxicity was not reduced by preincubation of the effector cells with plastic or iron carbonyl or by passage through cotton wool or agarose columns but was reduced by passage through nylon wool columns. Thymocytes were not cytotoxic to EB2 cells, and chronic lymphocytic leukaemia cells (of B cell characteristics) had reduced cytotoxicity compared with normal lymphocytes. Cells from various lymphoid organs of rats and guinea-pigs were also cytotoxic to EB2 cells with reactivity in spleen greater than or equal to blood greater than lymph nodes. Spleen cells from neonatally thymectomized rats had increased cytotoxicity compared with normal rat spleen cells, suggesting that T lymphocytes are not essential. The effector cell in rat spleen did not adhere to cotton wool or agarose columns, indicating some resemblance to its counterpart in human peripheral blood.", "contents": "Characterization of the normal lymphocyte population cytolytic to Burkitt's lymphoma cells of the EB2 cell line. The normal human lymphocyte population which exhibits \"spontaneous\" cytolysis of EB2 Burkitt's lymphoma cells has been characterized. The effector cell has EA and EAC' receptors but lacks E receptors and probably surface Ig. \"Spontaneous\" anti-EB2 cytotoxicity was not reduced by preincubation of the effector cells with plastic or iron carbonyl or by passage through cotton wool or agarose columns but was reduced by passage through nylon wool columns. Thymocytes were not cytotoxic to EB2 cells, and chronic lymphocytic leukaemia cells (of B cell characteristics) had reduced cytotoxicity compared with normal lymphocytes. Cells from various lymphoid organs of rats and guinea-pigs were also cytotoxic to EB2 cells with reactivity in spleen greater than or equal to blood greater than lymph nodes. Spleen cells from neonatally thymectomized rats had increased cytotoxicity compared with normal rat spleen cells, suggesting that T lymphocytes are not essential. The effector cell in rat spleen did not adhere to cotton wool or agarose columns, indicating some resemblance to its counterpart in human peripheral blood."}
{"id": "PMID:1084749", "title": "Altered lymphocyte reactivity in rheumatoid arthritis.", "content": "Blastogenic transformation of peripheral lymphocytes with phytohemagglutinin (PHA), conconavalin A (Con A), and pokeweed mitogen (PWM) was studied in 29 patients with rheumatoid arthritis (RA). The PHA response was depressed in a subgroup of RA patients with erosive disease. The Con A response was also depressed and paralleled the PHA response. The PWM response was not depressed. These results lend support to the hypothesis of a functional defect of cellular immunity in RA. It was shown that lymphocyte responsiveness to a single mitogen concentration is not an adequate assessment of the overall responsiveness of the lymphocytes tested.", "contents": "Altered lymphocyte reactivity in rheumatoid arthritis. Blastogenic transformation of peripheral lymphocytes with phytohemagglutinin (PHA), conconavalin A (Con A), and pokeweed mitogen (PWM) was studied in 29 patients with rheumatoid arthritis (RA). The PHA response was depressed in a subgroup of RA patients with erosive disease. The Con A response was also depressed and paralleled the PHA response. The PWM response was not depressed. These results lend support to the hypothesis of a functional defect of cellular immunity in RA. It was shown that lymphocyte responsiveness to a single mitogen concentration is not an adequate assessment of the overall responsiveness of the lymphocytes tested."}
{"id": "PMID:1084750", "title": "Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis.", "content": "Saline extracts of rabbit thymus were found to contain many nuclear antigens that reacted with antibodies in the sera of patients with systemic rheumatic diseases. Counterimmunoelectrophoresis (CIE) was used to detect antibodies to nuclear acidic protein (Sm), nuclear ribonucleoprotein (RNP), and antibody to nuclear antigen B, which was reported previously in Sj\u00f6gren's syndrome. All these nuclear antigens behaved as anions with different mobilities in CIE and could be distinguished from one another by the locations of the precipitin lines. They could also be distinguished by the facts that the nuclear RNP precipitin lines were abolished by digestion with ribonuclease whereas others were unaffected, and that Sm precipitin lines developed later than other precipitin lines. With this technique antibody to nuclear RNP was detected in 46% of patients with systemic lupus erythematosus (SLE) compared to 26% detected by the hemagglutination technique. Similarly the increased sensitivity of the CIE technique was able to show that antibody to B antigen was present in 12% of SLE patients, whereas this antibody was not detectable in the same group of patients by immunodiffusion. This study shows that CIE is a rapid and sensitive technique for detecting precipitating antibodies to a number of nuclear acidic antigens. Methods are described to identify the immunochemical specificities of the precipitin lines by the use of standard reference sera.", "contents": "Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis. Saline extracts of rabbit thymus were found to contain many nuclear antigens that reacted with antibodies in the sera of patients with systemic rheumatic diseases. Counterimmunoelectrophoresis (CIE) was used to detect antibodies to nuclear acidic protein (Sm), nuclear ribonucleoprotein (RNP), and antibody to nuclear antigen B, which was reported previously in Sj\u00f6gren's syndrome. All these nuclear antigens behaved as anions with different mobilities in CIE and could be distinguished from one another by the locations of the precipitin lines. They could also be distinguished by the facts that the nuclear RNP precipitin lines were abolished by digestion with ribonuclease whereas others were unaffected, and that Sm precipitin lines developed later than other precipitin lines. With this technique antibody to nuclear RNP was detected in 46% of patients with systemic lupus erythematosus (SLE) compared to 26% detected by the hemagglutination technique. Similarly the increased sensitivity of the CIE technique was able to show that antibody to B antigen was present in 12% of SLE patients, whereas this antibody was not detectable in the same group of patients by immunodiffusion. This study shows that CIE is a rapid and sensitive technique for detecting precipitating antibodies to a number of nuclear acidic antigens. Methods are described to identify the immunochemical specificities of the precipitin lines by the use of standard reference sera."}
{"id": "PMID:1084751", "title": "Obstructive pulmonary disease in rheumatoid arthritis.", "content": "Twenty-six of 43 patients (60.5%) with classic rheumatoid arthritis (RA) participating in a controlled, prospective study were found to have maximal midexpiratory flow rates (MMEFs) suggestive of obstructive pulmonary disease. Cigarette smokers with RA had significantly lower MMEFs than either nonsmokers with RA or smokers with degenerative joint disease. There was an increased prevalence of the Pi phenotype MS among the RA patients. Interstitial fibrosis, tobacco smoking, and protease inhibitor deficiencies were all significant factors in producing airway obstruction in patients with RA.", "contents": "Obstructive pulmonary disease in rheumatoid arthritis. Twenty-six of 43 patients (60.5%) with classic rheumatoid arthritis (RA) participating in a controlled, prospective study were found to have maximal midexpiratory flow rates (MMEFs) suggestive of obstructive pulmonary disease. Cigarette smokers with RA had significantly lower MMEFs than either nonsmokers with RA or smokers with degenerative joint disease. There was an increased prevalence of the Pi phenotype MS among the RA patients. Interstitial fibrosis, tobacco smoking, and protease inhibitor deficiencies were all significant factors in producing airway obstruction in patients with RA."}
{"id": "PMID:1084761", "title": "Isolation and identification of the major concanavalin A binding glycoproteins from murine-lymphocytes.", "content": "The major glycoproteins which bind concanavalin A have been isolated and identified from murine spleen cells, thymocytes,and purified thymus-derived (T) lymphocytes, and from the spleen cells of congenitally athymic (nude) mice. The cells were radiolabeled by lactoperoxidase catalyzed 125I iodination or by culturing the cells in media containing [3H]leucine or [3H]fucose. The cell membrane was solubilized with Nonidet P-40 and the concanavalin A binding proteins were isolated by affinity chromatography and analyzed according to their mobility on polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The major proteins from various lymphocyte preparations were identified by immunoprecipitation with specific antisera. The molecules coded by the histocompatibility-2 complex acted as concanavalin A binding proteins H-2K and H-2D were isolated from T lymphocytes, thymocytes, and bone marrow derived (B) lymphocytes. The Ia antigens were identified from B lymphocytes and tentatively identified from T lymphocytes. In addition to these H-2 complex proteins, immunoglobulin M and D on B lymphocytes also bound concanavalin A binding. All these glycoproteins have previously been identified as cell surface molecules. The presence of certain minor unidentified concanavalin A binding proteins on lymphoid cells is indicated.", "contents": "Isolation and identification of the major concanavalin A binding glycoproteins from murine-lymphocytes. The major glycoproteins which bind concanavalin A have been isolated and identified from murine spleen cells, thymocytes,and purified thymus-derived (T) lymphocytes, and from the spleen cells of congenitally athymic (nude) mice. The cells were radiolabeled by lactoperoxidase catalyzed 125I iodination or by culturing the cells in media containing [3H]leucine or [3H]fucose. The cell membrane was solubilized with Nonidet P-40 and the concanavalin A binding proteins were isolated by affinity chromatography and analyzed according to their mobility on polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The major proteins from various lymphocyte preparations were identified by immunoprecipitation with specific antisera. The molecules coded by the histocompatibility-2 complex acted as concanavalin A binding proteins H-2K and H-2D were isolated from T lymphocytes, thymocytes, and bone marrow derived (B) lymphocytes. The Ia antigens were identified from B lymphocytes and tentatively identified from T lymphocytes. In addition to these H-2 complex proteins, immunoglobulin M and D on B lymphocytes also bound concanavalin A binding. All these glycoproteins have previously been identified as cell surface molecules. The presence of certain minor unidentified concanavalin A binding proteins on lymphoid cells is indicated."}
{"id": "PMID:1084762", "title": "Human plasma kallikreins and their inhibition by amidino compounds.", "content": "Human plasma kallikreins (EC 3.4.21.8) were purified as three distinct enzyme entities which hydrolyzed arginine esters and were active in releasing kinin from heated human plasma as measured by guinea pig ileum contraction bio-assay. The three enzymatically active fractions were termed as 19 S, 7 S-I and 7 S-II kallikreins. They represented purifications of 262- 2200- and 110-fold, respectively. These enzyme activities showed differences in physicochemical and biochemical properties as it appears from their elution profile on Sephadex G-200 and DEAE-cellulose columns, affinity for substrates and susceptibility of inhibition by various protease inhibitors such as trasylol and soya bean trypsin inhibitor. The data suggest that all these three enzyme preparations were most likely kallikreins. All these three enzymes (19 S, 7 S-I and 7 S-II) were inhibited by a series of amidino compounds competitively. Diamidines consisting of two amidinophenyl residues linked in para position by molecular bridge were comparatively stronger inhibitors of all of three enzymes than those linked in meta position and those having single ring structure. The possibility that some of these amidino compounds might prove to be useful for treatment of disease states where the kallikrein-kinin system plays a role, is discussed.", "contents": "Human plasma kallikreins and their inhibition by amidino compounds. Human plasma kallikreins (EC 3.4.21.8) were purified as three distinct enzyme entities which hydrolyzed arginine esters and were active in releasing kinin from heated human plasma as measured by guinea pig ileum contraction bio-assay. The three enzymatically active fractions were termed as 19 S, 7 S-I and 7 S-II kallikreins. They represented purifications of 262- 2200- and 110-fold, respectively. These enzyme activities showed differences in physicochemical and biochemical properties as it appears from their elution profile on Sephadex G-200 and DEAE-cellulose columns, affinity for substrates and susceptibility of inhibition by various protease inhibitors such as trasylol and soya bean trypsin inhibitor. The data suggest that all these three enzyme preparations were most likely kallikreins. All these three enzymes (19 S, 7 S-I and 7 S-II) were inhibited by a series of amidino compounds competitively. Diamidines consisting of two amidinophenyl residues linked in para position by molecular bridge were comparatively stronger inhibitors of all of three enzymes than those linked in meta position and those having single ring structure. The possibility that some of these amidino compounds might prove to be useful for treatment of disease states where the kallikrein-kinin system plays a role, is discussed."}
{"id": "PMID:1084763", "title": "Delayed fluorescence from Rhodopseudomonas sphaeroides following single flashes.", "content": "Delayed fluorescence from Rhodopseudomonas sphaeroides chromatophores was studied with the use of short flashes for excitation. Although the delayed fluorescence probably arises from a back-reaction between the oxidized reaction center bacteriochlorophyll complex (P+) and the reduced electron acceptor (X-), the decay of delayed fluorescence after a flash is much faster (tau1/2 approximately 120 mus) than the decay of P+X-. The rapid decay of delayed fluorescence is not due to the uptake of a proton from the solution, nor to a change in membrane potential. It correlates with small optical absorbance changes at 450 and 770 nm which could reflect a change in the state of X-. The intensity of the delayed fluorescence is 11-18-fold greater if the excitation flashes are spaced 2 s apart than it is if they are 30 s apart. The enhancement of delayed fluorescence at high flash repetition rates occurs only at redox potentials which are low enough (less than +240 mV) so that electron donors are available to reduce P+X- to PX- in part of the reaction center population. The enhancement decays between flashes as PX- is reoxidized to PX, as measured by the recovery of photochemical activity. Evidently, the reduction of P+X- to PX- leads to the storage of free energy that can be used on a subsequent flash to promote delayed fluorescence. The reduction of P+X- also is associated with a carotenoid spectral shift which decays as PX- is reoxidized to PX. Although this suggests that the free energy which supports the delayed fluorescence might be stored as a membrane potential, the ionophore gramicidin D only partially inhibits the enhancement of delayed fluorescence. With widely separated flashes, gramicidin has no effect on delayed fluorescence. At redox potentials low enough to keep X fully reduced, delayed fluorescence of the type described above does not occur, but one can detect weak luminescence which probably is due to phosphorescence of a protoporphyrin.", "contents": "Delayed fluorescence from Rhodopseudomonas sphaeroides following single flashes. Delayed fluorescence from Rhodopseudomonas sphaeroides chromatophores was studied with the use of short flashes for excitation. Although the delayed fluorescence probably arises from a back-reaction between the oxidized reaction center bacteriochlorophyll complex (P+) and the reduced electron acceptor (X-), the decay of delayed fluorescence after a flash is much faster (tau1/2 approximately 120 mus) than the decay of P+X-. The rapid decay of delayed fluorescence is not due to the uptake of a proton from the solution, nor to a change in membrane potential. It correlates with small optical absorbance changes at 450 and 770 nm which could reflect a change in the state of X-. The intensity of the delayed fluorescence is 11-18-fold greater if the excitation flashes are spaced 2 s apart than it is if they are 30 s apart. The enhancement of delayed fluorescence at high flash repetition rates occurs only at redox potentials which are low enough (less than +240 mV) so that electron donors are available to reduce P+X- to PX- in part of the reaction center population. The enhancement decays between flashes as PX- is reoxidized to PX, as measured by the recovery of photochemical activity. Evidently, the reduction of P+X- to PX- leads to the storage of free energy that can be used on a subsequent flash to promote delayed fluorescence. The reduction of P+X- also is associated with a carotenoid spectral shift which decays as PX- is reoxidized to PX. Although this suggests that the free energy which supports the delayed fluorescence might be stored as a membrane potential, the ionophore gramicidin D only partially inhibits the enhancement of delayed fluorescence. With widely separated flashes, gramicidin has no effect on delayed fluorescence. At redox potentials low enough to keep X fully reduced, delayed fluorescence of the type described above does not occur, but one can detect weak luminescence which probably is due to phosphorescence of a protoporphyrin."}
{"id": "PMID:1084764", "title": "Cordycepin and early effects of estradiol on the immature rat uterus.", "content": "Estradiol induces the synthesis of a specific protein fraction (IP) in the uterus of the immature rat. The injection of cordycepin (3' deoxyadenosine), an inhibitor of poly A synthesis, inhibits the synthesis of IP. This fact suggests that one of the earliest effects of estrogen is the production of Hn-RNA poly-A relative to IP. Moreover, using electron microscopy, the stimulation by estradiol of the nucleolus of the immature rat uterine epithelium has been shown. Cordycepin does not affect this stimulation to any appreciable extent. Biochemical studies (incorporation of radioactive stracers into NRA, affinity chromatography on poly U-Sepharose) carried out in parallel with and under conditions comparable to those used in electron microscopy show that cordycepin does not greatly affect the increase in ribosomal RNA observed under the effect of estradiol. The blocking of IP by cordycepin and the lack of inhibition at the nucleolus level under the same conditions, show that the two early effects of the action of estrogen on the immature rat uterus are not directly correlated.", "contents": "Cordycepin and early effects of estradiol on the immature rat uterus. Estradiol induces the synthesis of a specific protein fraction (IP) in the uterus of the immature rat. The injection of cordycepin (3' deoxyadenosine), an inhibitor of poly A synthesis, inhibits the synthesis of IP. This fact suggests that one of the earliest effects of estrogen is the production of Hn-RNA poly-A relative to IP. Moreover, using electron microscopy, the stimulation by estradiol of the nucleolus of the immature rat uterine epithelium has been shown. Cordycepin does not affect this stimulation to any appreciable extent. Biochemical studies (incorporation of radioactive stracers into NRA, affinity chromatography on poly U-Sepharose) carried out in parallel with and under conditions comparable to those used in electron microscopy show that cordycepin does not greatly affect the increase in ribosomal RNA observed under the effect of estradiol. The blocking of IP by cordycepin and the lack of inhibition at the nucleolus level under the same conditions, show that the two early effects of the action of estrogen on the immature rat uterus are not directly correlated."}
{"id": "PMID:1084766", "title": "Sarcomere length dispersion in single skeletal muscle fibers and fiber bundles.", "content": "Light diffraction patterns produced by single skeletal muscle fibers and small fiber bundles of Rana pipiens semitendinosus have been examined at rest and during tetanic contraction. The muscle diffraction patterns were recorded with a vidicon camera interfaced to a minicomputer. Digitized video output was analyzed on-line to determine mean sarcomere length, line intensity, and the distribution of sarcomere lengths. The occurrence of first-order line intensity and peak amplitude maxima at approximately 3.0 mum is interpreted in terms of simple scattering theory. Measurements made along the length of a singel fiber reveal small variations in calculated mean sarcomere length (SD about 1.2%) and its percent dispersion (2.1% +/- 0.8%). Dispersion in small multifiber preparations increases approximately linearly with fiber number (about 0.2% per fiber) to a maximum of 8-10% in large bundles. Dispersion measurements based upon diffraction line analysis are comparable to SDs calculated from length distribution histograms obtained by light micrography of the fiber. First-order line intensity decreases by about 40% during tetanus; larger multifibered bundles exhibit substantial increases in sarcomere dispersion during contraction, but single fibers show no appreciable dispersion change. These results suggest the occurrence of asynchronous static or dynamic axial disordering of thick filaments, with a persistence in long range order of sarcomere spacing during contraction in single fibers.", "contents": "Sarcomere length dispersion in single skeletal muscle fibers and fiber bundles. Light diffraction patterns produced by single skeletal muscle fibers and small fiber bundles of Rana pipiens semitendinosus have been examined at rest and during tetanic contraction. The muscle diffraction patterns were recorded with a vidicon camera interfaced to a minicomputer. Digitized video output was analyzed on-line to determine mean sarcomere length, line intensity, and the distribution of sarcomere lengths. The occurrence of first-order line intensity and peak amplitude maxima at approximately 3.0 mum is interpreted in terms of simple scattering theory. Measurements made along the length of a singel fiber reveal small variations in calculated mean sarcomere length (SD about 1.2%) and its percent dispersion (2.1% +/- 0.8%). Dispersion in small multifiber preparations increases approximately linearly with fiber number (about 0.2% per fiber) to a maximum of 8-10% in large bundles. Dispersion measurements based upon diffraction line analysis are comparable to SDs calculated from length distribution histograms obtained by light micrography of the fiber. First-order line intensity decreases by about 40% during tetanus; larger multifibered bundles exhibit substantial increases in sarcomere dispersion during contraction, but single fibers show no appreciable dispersion change. These results suggest the occurrence of asynchronous static or dynamic axial disordering of thick filaments, with a persistence in long range order of sarcomere spacing during contraction in single fibers."}
{"id": "PMID:1084767", "title": "Insulin binding of acute lymphocytic leukemia cells.", "content": "Because of differences in insulin binding of cultured lymphoic cell lines, T- and B-cell surface receptor and 125I-insulin binding studies were performed on the bone marrow and peripheral blood leukocytes of 13 children with active acute lymphocytec leukemia. Based on surface receptors, nine patients had null-cell disease and four had T-cell disease. The mean per cent insulin binding of the bone marrow cells from the null-cell patients was 10.0% +/- 8.1 and from the T-cell patients was 0.18% +/- 0.13. The mean insulin binding of the cell suspensions of the peripheral blood from the null-cell patients was 7.3% +/- 7.5 and 0.07% +/- 0.06 from the T-cell patients. Displacement studies with nonradioactive insulin indicated that null leukemic cells bore specific binding sites. These results indicated that there may be metabolic as well as surface membrane heterogeneity among the acute lymphocytic leukemias of childhood.", "contents": "Insulin binding of acute lymphocytic leukemia cells. Because of differences in insulin binding of cultured lymphoic cell lines, T- and B-cell surface receptor and 125I-insulin binding studies were performed on the bone marrow and peripheral blood leukocytes of 13 children with active acute lymphocytec leukemia. Based on surface receptors, nine patients had null-cell disease and four had T-cell disease. The mean per cent insulin binding of the bone marrow cells from the null-cell patients was 10.0% +/- 8.1 and from the T-cell patients was 0.18% +/- 0.13. The mean insulin binding of the cell suspensions of the peripheral blood from the null-cell patients was 7.3% +/- 7.5 and 0.07% +/- 0.06 from the T-cell patients. Displacement studies with nonradioactive insulin indicated that null leukemic cells bore specific binding sites. These results indicated that there may be metabolic as well as surface membrane heterogeneity among the acute lymphocytic leukemias of childhood."}
{"id": "PMID:1084768", "title": "A comparison of granulopoiesis in culture from blood and marrow cells of nonleukemic individuals and patients with acute leukemia.", "content": "The objective of this study was to compare the concentration of committed granulopoietic progenitor cells (CFU-C) in marrow and blood. For individuals without leukemia, a highly significant correlation was observed between the concentration of CFU-C obtained from the two sites. However, CFU-C in blood had a slower sedimentation velocity than that reported for marrow and were found not to be in the DNA synthetic phase of the cycle using the tritiated thymidine suicide tehcnique. In patients with acute leukemia, no correlation was observed between concentrations of CFU-C in marrow and peripheral blood, regardless of whether the patients were newly diagnosed, in remission, or in relapse. We concluded that studies of the peripheral blood do not yield the same information in respect to granulopoietic progenitor cells as do studies of the marrow.", "contents": "A comparison of granulopoiesis in culture from blood and marrow cells of nonleukemic individuals and patients with acute leukemia. The objective of this study was to compare the concentration of committed granulopoietic progenitor cells (CFU-C) in marrow and blood. For individuals without leukemia, a highly significant correlation was observed between the concentration of CFU-C obtained from the two sites. However, CFU-C in blood had a slower sedimentation velocity than that reported for marrow and were found not to be in the DNA synthetic phase of the cycle using the tritiated thymidine suicide tehcnique. In patients with acute leukemia, no correlation was observed between concentrations of CFU-C in marrow and peripheral blood, regardless of whether the patients were newly diagnosed, in remission, or in relapse. We concluded that studies of the peripheral blood do not yield the same information in respect to granulopoietic progenitor cells as do studies of the marrow."}
{"id": "PMID:1084769", "title": "Fetal hemopoiesis in diffusion chamber cultures. III. The effect of neutropenia.", "content": "Proliferation and differentiation of granulocytes, macrophages, and both myeloid committed (CFC) and pluripotent (CFU) stem cells in diffusion chamber (DC) cultures of fetal liver were studied in order to evaluate the role of circulating humoral factors in the control of fetal myelopoiesis. When DC with fetal liver cells were implanted into mice rendered neutropenic by pretreatment with cyclophosphamide, more granulocytes and CFC were produced through day 10 as compared to DC implanted into saline pretreated control hosts. A difference in CFU recovery from fetal liver suspensions grown in DC implanted into neutropenic and control hosts was not seen until day 10. Serum CSF concentrations were increased in neutropenic as compared to control host mice 2 and 3 days after implantation of DC. Levels of serum inhibitors of colony growth showed marked variability but, in general, were similar in both groups. These data provide evidence that fetal CFC and fetal myelopoiesis are influenced by a circulating humoral factor present in neutropenic serum. CSF may be the factor, although the data presented in this paper do not establish this with any certainty.", "contents": "Fetal hemopoiesis in diffusion chamber cultures. III. The effect of neutropenia. Proliferation and differentiation of granulocytes, macrophages, and both myeloid committed (CFC) and pluripotent (CFU) stem cells in diffusion chamber (DC) cultures of fetal liver were studied in order to evaluate the role of circulating humoral factors in the control of fetal myelopoiesis. When DC with fetal liver cells were implanted into mice rendered neutropenic by pretreatment with cyclophosphamide, more granulocytes and CFC were produced through day 10 as compared to DC implanted into saline pretreated control hosts. A difference in CFU recovery from fetal liver suspensions grown in DC implanted into neutropenic and control hosts was not seen until day 10. Serum CSF concentrations were increased in neutropenic as compared to control host mice 2 and 3 days after implantation of DC. Levels of serum inhibitors of colony growth showed marked variability but, in general, were similar in both groups. These data provide evidence that fetal CFC and fetal myelopoiesis are influenced by a circulating humoral factor present in neutropenic serum. CSF may be the factor, although the data presented in this paper do not establish this with any certainty."}
{"id": "PMID:1084771", "title": "New data on the vision of South American Indians.", "content": "A total of 466 males and 437 females from four Brazilian Indian tribes were tested for color blindness with Ishihara's plates. Defective persons were found in three of the four tribes, but when these and other groups are considered the evidence suggests that the frequency of this trait is lower among Amerindians than among Caucasian populations. Visual acuity tests were performed on 296 Yanomama Indians. Their visual acuity was apparently not as sharp as that of the Cayapo or Xavante. But the scarcity among the Yanomama of persons with serious visual impairment of subcutaneous nodules suggests that the focus of onchocerciasis discovered among them is of recent origin.", "contents": "New data on the vision of South American Indians. A total of 466 males and 437 females from four Brazilian Indian tribes were tested for color blindness with Ishihara's plates. Defective persons were found in three of the four tribes, but when these and other groups are considered the evidence suggests that the frequency of this trait is lower among Amerindians than among Caucasian populations. Visual acuity tests were performed on 296 Yanomama Indians. Their visual acuity was apparently not as sharp as that of the Cayapo or Xavante. But the scarcity among the Yanomama of persons with serious visual impairment of subcutaneous nodules suggests that the focus of onchocerciasis discovered among them is of recent origin."}
{"id": "PMID:1084773", "title": "The effect of quinidine on membrane electrical activity in frog auricular fibres studied by current and voltage clamp.", "content": "1 The action of quinidine sulphate 50 muM has been investigated on frog auricular trabeculae transmembrane currents recorded with a double sucrose gap apparatus. Results were obtained either in current or in voltage clamp conditions. 2 Quinidine modified the time course of repetitive activity elicited by long lasting depolarizing currents and reduced the range of current over which repetitive activity could be triggered, eventually abolishing repetitive responses altogether. 3 Several authors have emphasized the limitations of the voltage clamp method. Taking into account these limitations, the numerical values of the parameters obtained in the present work must not be considered as exact values but may be interpreted as indicators of the variations of the parameters. 4 The results are in agreement with previous findings that the main features of the action of quinidine are to produce (a) a reduced maximum rate of depolarization (MRD), (b) a reduced total amplitude of action potential, (c) a flattening of the plateau of the action potential, (d) a slight prolongation of the tail of the action potential, (e) an increased effective refractory period without greatly prolonging action potential duration, (f) no change of resting potential and of 50% repolarization time. 5 The analysis of ionic conductances has provided explanations for the above effects. 6 Quinidine reduced the reactivation kinetics of the sodium inward current, and decreased sodium conductance and the steady state of activation. These effects account for (a) and (b). 7 Quinidine increased the activation and inactivation time constants of sodium conductances, which account in part for (e). 8 Quinidine delayed reactivation of slow inward current, reduced calcium conductance, and decreased the steady state of activation of calcium conductance. These effects could account for (c). 9 The amplitudes of the two components of the delayed conductances responsible for repolarization were decreased by quinidine, and the time constant of activation for the faster of the two was slowed. These effects could account for (d) and in part for (e).", "contents": "The effect of quinidine on membrane electrical activity in frog auricular fibres studied by current and voltage clamp. 1 The action of quinidine sulphate 50 muM has been investigated on frog auricular trabeculae transmembrane currents recorded with a double sucrose gap apparatus. Results were obtained either in current or in voltage clamp conditions. 2 Quinidine modified the time course of repetitive activity elicited by long lasting depolarizing currents and reduced the range of current over which repetitive activity could be triggered, eventually abolishing repetitive responses altogether. 3 Several authors have emphasized the limitations of the voltage clamp method. Taking into account these limitations, the numerical values of the parameters obtained in the present work must not be considered as exact values but may be interpreted as indicators of the variations of the parameters. 4 The results are in agreement with previous findings that the main features of the action of quinidine are to produce (a) a reduced maximum rate of depolarization (MRD), (b) a reduced total amplitude of action potential, (c) a flattening of the plateau of the action potential, (d) a slight prolongation of the tail of the action potential, (e) an increased effective refractory period without greatly prolonging action potential duration, (f) no change of resting potential and of 50% repolarization time. 5 The analysis of ionic conductances has provided explanations for the above effects. 6 Quinidine reduced the reactivation kinetics of the sodium inward current, and decreased sodium conductance and the steady state of activation. These effects account for (a) and (b). 7 Quinidine increased the activation and inactivation time constants of sodium conductances, which account in part for (e). 8 Quinidine delayed reactivation of slow inward current, reduced calcium conductance, and decreased the steady state of activation of calcium conductance. These effects could account for (c). 9 The amplitudes of the two components of the delayed conductances responsible for repolarization were decreased by quinidine, and the time constant of activation for the faster of the two was slowed. These effects could account for (d) and in part for (e)."}
{"id": "PMID:1084774", "title": "125Iodinated fibrinogen uptake in inflammatory joint disease as a complicating factor in the detection of deep vein thrombosis.", "content": "An enhanced uptake of 125I-fibrinogen observed in the arthritic knee joint of a patient suspected of deep vein thrombosis was confirmed in a number of patients with inflammatory joint disease in the absence of thrombosis. Whilst this is a potentially serious source of false positive diagnoses, the possibility also exists that this phenomenon could be used to evaluate the degree to which active disease is present in the joints of patients suffering from inflammatory disease.", "contents": "125Iodinated fibrinogen uptake in inflammatory joint disease as a complicating factor in the detection of deep vein thrombosis. An enhanced uptake of 125I-fibrinogen observed in the arthritic knee joint of a patient suspected of deep vein thrombosis was confirmed in a number of patients with inflammatory joint disease in the absence of thrombosis. Whilst this is a potentially serious source of false positive diagnoses, the possibility also exists that this phenomenon could be used to evaluate the degree to which active disease is present in the joints of patients suffering from inflammatory disease."}
{"id": "PMID:1084780", "title": "Interdigitating reticulum cells in the popliteal lymph node of the rat. An ultrastructural and cytochemical study.", "content": "Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is considered to be an argument in favour of the common origin of IDC's and macrophages. Ultrahistochemical studies with horseradish peroxidase (HRP) reveal no phagocytotic capacity of IDC's. After perfusion fixation containing ruthenium red (RR) the surface coat stains heavily: RR is also found deep in the membrane invaginations of the IDC, indicating the presence of polyanionic sialoglycoproteins. The post-capillary-venules (PVC) are very  permeable to both HRP and RR. The phosphotungstic acid-chromic acid stain (PTA-CrA) also reveals glycoproteins in the surface coat; these glycoproteins are susceptible to alpha-neuraminidase, whereas glycoproteins in the Golgi complexes, lysosomes and in the vesicular complexes of IDC are not. The glycoproteins of the latter are susceptible to 0.1 N NaOH. These findings indicate that IDC produce different kinds of glycoprotein, one of which may be secreted and act as a factor for stimulating peripheral T-lymphocytes. Intimate contact between IDC's and PCV's could be observed. It is therefore conceivable that IDC's play an important role in the homing of T-lymphocytes.", "contents": "Interdigitating reticulum cells in the popliteal lymph node of the rat. An ultrastructural and cytochemical study. Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is considered to be an argument in favour of the common origin of IDC's and macrophages. Ultrahistochemical studies with horseradish peroxidase (HRP) reveal no phagocytotic capacity of IDC's. After perfusion fixation containing ruthenium red (RR) the surface coat stains heavily: RR is also found deep in the membrane invaginations of the IDC, indicating the presence of polyanionic sialoglycoproteins. The post-capillary-venules (PVC) are very  permeable to both HRP and RR. The phosphotungstic acid-chromic acid stain (PTA-CrA) also reveals glycoproteins in the surface coat; these glycoproteins are susceptible to alpha-neuraminidase, whereas glycoproteins in the Golgi complexes, lysosomes and in the vesicular complexes of IDC are not. The glycoproteins of the latter are susceptible to 0.1 N NaOH. These findings indicate that IDC produce different kinds of glycoprotein, one of which may be secreted and act as a factor for stimulating peripheral T-lymphocytes. Intimate contact between IDC's and PCV's could be observed. It is therefore conceivable that IDC's play an important role in the homing of T-lymphocytes."}
{"id": "PMID:1084781", "title": "Autoradiographic localization of 3H-5-HTP and 3H-5-HT in the pineal organ and circumventricular areas in the rainbow trout, Salmo gairdneri Richardson.", "content": "The time course of incorporation and cellular localization of 3H-5-hydroxytryptophan (3H-5-HTP) and 3H-5-hydroxytryptamine (3H-5-HT) in the pineal and some brain regions in rainbow trout, Salmo gairdneri, were studied by quantitative and qualitative autoradiography. Among the tissues examined, the pineal shows the highest and most rapid uptake of the two isotopes. Maximum incorporation of 3H-5-HTP is achieved by 2 h and that of 3H-5-HT by 20 minutes post injection. At the end of the six-hour experimental period, a significantly high amount of radioactivity is still detectable in the pineal. The results indicate a much slower turnover of the two indoles, especially 5-HTP, in the trout than is known for mammalian tissues. Both the ependymal supporting cells and the receptor cells of the pineal localize these isotopes. In constant, the intrapienal neurons remain unlabeled. This is taken to suggest lack of capacity of these cells to metabolize 5-HTP and 5-HT. In the circumventricular regions, the two indoles occur in the ependyma of the recessus lateralis and the recessus praeopticus. The label is also localized in the neuropil and the neurons of the nuclei recessus lateralis and praeopticus. Semiquantitative estimates reveal a significant labeling of these areas only 20 minutes after injection, although a weak but inconsistent labeling of the ependyma is evident at 5 minutes. The significance of these results is discussed in regard to (a) normal capacity of circumventricular areas to metabolize indoles and (b) a possible chemical interaction between the pineal and the brain involving a direct pineal-cerebrospinal fluid pathway.", "contents": "Autoradiographic localization of 3H-5-HTP and 3H-5-HT in the pineal organ and circumventricular areas in the rainbow trout, Salmo gairdneri Richardson. The time course of incorporation and cellular localization of 3H-5-hydroxytryptophan (3H-5-HTP) and 3H-5-hydroxytryptamine (3H-5-HT) in the pineal and some brain regions in rainbow trout, Salmo gairdneri, were studied by quantitative and qualitative autoradiography. Among the tissues examined, the pineal shows the highest and most rapid uptake of the two isotopes. Maximum incorporation of 3H-5-HTP is achieved by 2 h and that of 3H-5-HT by 20 minutes post injection. At the end of the six-hour experimental period, a significantly high amount of radioactivity is still detectable in the pineal. The results indicate a much slower turnover of the two indoles, especially 5-HTP, in the trout than is known for mammalian tissues. Both the ependymal supporting cells and the receptor cells of the pineal localize these isotopes. In constant, the intrapienal neurons remain unlabeled. This is taken to suggest lack of capacity of these cells to metabolize 5-HTP and 5-HT. In the circumventricular regions, the two indoles occur in the ependyma of the recessus lateralis and the recessus praeopticus. The label is also localized in the neuropil and the neurons of the nuclei recessus lateralis and praeopticus. Semiquantitative estimates reveal a significant labeling of these areas only 20 minutes after injection, although a weak but inconsistent labeling of the ependyma is evident at 5 minutes. The significance of these results is discussed in regard to (a) normal capacity of circumventricular areas to metabolize indoles and (b) a possible chemical interaction between the pineal and the brain involving a direct pineal-cerebrospinal fluid pathway."}
{"id": "PMID:1084794", "title": "Parasite antigens.", "content": "The currently available preparations used as antigen in the serological investigation of parasitic diseases are ill-defined heterogeneous mixtures, and there is an evident need for better characterized reagents. Antigens of different parasite species (schistosomes, filariae, trypanosomes, and plasmodia) are discussed and parasite sources enumerated. Modern methods for the preparation of antigenic extracts and their fractionation are described, together with certain guidelines as to their biochemical characterization and their immunological activity. In order to implement this endeavour and to make better use of serological techniques in parasitic diseases, proposals are made concerning collaborative research and field application among a number of laboratories on schistosome, onchocercal, trypanosome, and plasmodial antigens.", "contents": "Parasite antigens. The currently available preparations used as antigen in the serological investigation of parasitic diseases are ill-defined heterogeneous mixtures, and there is an evident need for better characterized reagents. Antigens of different parasite species (schistosomes, filariae, trypanosomes, and plasmodia) are discussed and parasite sources enumerated. Modern methods for the preparation of antigenic extracts and their fractionation are described, together with certain guidelines as to their biochemical characterization and their immunological activity. In order to implement this endeavour and to make better use of serological techniques in parasitic diseases, proposals are made concerning collaborative research and field application among a number of laboratories on schistosome, onchocercal, trypanosome, and plasmodial antigens."}
{"id": "PMID:1084795", "title": "Epidemiology of chronic non-specific respiratory diseases.", "content": "The current state of research into the epidemiology of chronic non-specific respiratory diseases (CNSRD) is reviewed. Recommendations are made on the definitions of CNSRD for use in epidemiological studies, and various aspects of the etiology and natural history of CNSRD are identified as requiring further investigation. The need for standardization of investigative methods is emphasized. Since smoking is such an important factor in the etiology of CNSRD, it is recommended that efforts be made to discourage children from taking up the habit.", "contents": "Epidemiology of chronic non-specific respiratory diseases. The current state of research into the epidemiology of chronic non-specific respiratory diseases (CNSRD) is reviewed. Recommendations are made on the definitions of CNSRD for use in epidemiological studies, and various aspects of the etiology and natural history of CNSRD are identified as requiring further investigation. The need for standardization of investigative methods is emphasized. Since smoking is such an important factor in the etiology of CNSRD, it is recommended that efforts be made to discourage children from taking up the habit."}
{"id": "PMID:1084796", "title": "Nomenclature for factors of the HLA system.", "content": "A Nomenclature Committee composed of geneticists and immunologists, including specialists in tissue typing, has met after each of the Histocompatibility Workshops beginning with the Third Workshop in 1967. The Committee, in part under the auspices of the World Health Organization and the International Union of Immunological Societies (WHO/IUIS), met after the Sixth Workshop in Aarhus in July 1975. The expanding knowledge of the genetics of the major histocompatibility system of man has necessitated a revision of the terminology for the HLA region following the principles established in previous reports. This has been done with as few changes as possible.", "contents": "Nomenclature for factors of the HLA system. A Nomenclature Committee composed of geneticists and immunologists, including specialists in tissue typing, has met after each of the Histocompatibility Workshops beginning with the Third Workshop in 1967. The Committee, in part under the auspices of the World Health Organization and the International Union of Immunological Societies (WHO/IUIS), met after the Sixth Workshop in Aarhus in July 1975. The expanding knowledge of the genetics of the major histocompatibility system of man has necessitated a revision of the terminology for the HLA region following the principles established in previous reports. This has been done with as few changes as possible."}
{"id": "PMID:1084797", "title": "Distribution of the snail Biomphalaria glabrata, intermediate host of Schistosoma mansoni, within a St Lucian field habitat.", "content": "A total of 6360 mud samples were obtained, in 62 collections made with an exhaustive sampling device, from banana drains on the West Indian island of St Lucia during fortnightly samplings over a 2(1/2)-year period. Analysis of counts of the snail Biomphalaria glabrata from these samples showed that this species had a contagious distribution. This finding is consistent with other evidence that banana drains form a rigorous habitat for B. glabrata. Its distribution was more contagious than that of Oncomelania quadrasi in certain Philippine habitats and several species of aquatic snail in various African irrigation canals. The exact transformation for normalizing the snail counts for standard statistical techniques was z = x(0.287) but the more convenient cube root transformation is probably adequate. However, if too few snails are collected (15 or fewer per 100 samples) or if the frequency distribution of snail counts is discontinuous, with too many widely separated high frequency counts, neither transformation will be entirely satisfactory.", "contents": "Distribution of the snail Biomphalaria glabrata, intermediate host of Schistosoma mansoni, within a St Lucian field habitat. A total of 6360 mud samples were obtained, in 62 collections made with an exhaustive sampling device, from banana drains on the West Indian island of St Lucia during fortnightly samplings over a 2(1/2)-year period. Analysis of counts of the snail Biomphalaria glabrata from these samples showed that this species had a contagious distribution. This finding is consistent with other evidence that banana drains form a rigorous habitat for B. glabrata. Its distribution was more contagious than that of Oncomelania quadrasi in certain Philippine habitats and several species of aquatic snail in various African irrigation canals. The exact transformation for normalizing the snail counts for standard statistical techniques was z = x(0.287) but the more convenient cube root transformation is probably adequate. However, if too few snails are collected (15 or fewer per 100 samples) or if the frequency distribution of snail counts is discontinuous, with too many widely separated high frequency counts, neither transformation will be entirely satisfactory."}
{"id": "PMID:1084798", "title": "Blinding and non-blinding trachoma: assessment of intensity of upper tarsal inflammatory disease and disabling lesions.", "content": "A simplified method of evaluating both the intensity of active inflammatory disease and the visually disabling lesions of trachoma is presented. Evaluation of the active disease is based on the scoring of clinical signs, lymphoid follicles, and papillary hypertrophy. The irreversible lesions of trachoma are classified as conjunctival scarring, trichiasis and/or entropion, and corneal scarring.", "contents": "Blinding and non-blinding trachoma: assessment of intensity of upper tarsal inflammatory disease and disabling lesions. A simplified method of evaluating both the intensity of active inflammatory disease and the visually disabling lesions of trachoma is presented. Evaluation of the active disease is based on the scoring of clinical signs, lymphoid follicles, and papillary hypertrophy. The irreversible lesions of trachoma are classified as conjunctival scarring, trichiasis and/or entropion, and corneal scarring."}
{"id": "PMID:1084799", "title": "Hepatitis B antigen, antigen subtypes, and hepatitis B antibody in normal subjects and patients with liver disease.", "content": "The relative sensitivities of counterimmunoelectrophoresis (CIE) and haemagglutination assays for the detection of hepatitis B surface antigen (HB(s)Ag) and antibodies (anti-HB(s)) were compared. Twelve scientists from ten countries in Asia, Africa and the Pacific region participated in the study. The participants provided serum samples from 15 953 subjects comprising patients with acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC), as well as blood donors and other normal individuals. For the detection of HB(s)Ag in a reference panel serum, immune adherence haemagglutination (IAHA) was slightly more sensitive than passive haemagglutination inhibition (PHI); CIE was the least sensitive. Mean HB(s)Ag frequencies in patients with acute hepatitis, chronic hepatitis, cirrhosis, and HCC were significantly higher than in healthy controls. Passive haemagglutination (PHA) was more sensitive than CIE for the detection of anti-HB(s). The frequency of anti-HB(s) in patients with HCC was significantly lower than that in the other groups. Mean anti-HB(s) frequencies in patients with acute hepatitis, chronic hepatitis, and cirrhosis were not significantly different from that in normal subjects. Subtyping of HB(s)Ag was performed by PHI. Among asymptomatic carriers the predominant HB(s)Ag subtype in northeast Asia was adr.In India, ayw predominated in carriers, with the demarcation between adr and ayw occurring west of Burma. In West Africa the only subtype detected was ayw, but in East Africa the majority subtype was adw. The r subtype was found only in Asian populations east of India and in Western Pacific populations. In Papua New Guinea all four subtypes were identified. With one possible exception, the subtypes of HB(s)Ag-positive patients with liver disease reflected the predominant type in each geographic location.", "contents": "Hepatitis B antigen, antigen subtypes, and hepatitis B antibody in normal subjects and patients with liver disease. The relative sensitivities of counterimmunoelectrophoresis (CIE) and haemagglutination assays for the detection of hepatitis B surface antigen (HB(s)Ag) and antibodies (anti-HB(s)) were compared. Twelve scientists from ten countries in Asia, Africa and the Pacific region participated in the study. The participants provided serum samples from 15 953 subjects comprising patients with acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC), as well as blood donors and other normal individuals. For the detection of HB(s)Ag in a reference panel serum, immune adherence haemagglutination (IAHA) was slightly more sensitive than passive haemagglutination inhibition (PHI); CIE was the least sensitive. Mean HB(s)Ag frequencies in patients with acute hepatitis, chronic hepatitis, cirrhosis, and HCC were significantly higher than in healthy controls. Passive haemagglutination (PHA) was more sensitive than CIE for the detection of anti-HB(s). The frequency of anti-HB(s) in patients with HCC was significantly lower than that in the other groups. Mean anti-HB(s) frequencies in patients with acute hepatitis, chronic hepatitis, and cirrhosis were not significantly different from that in normal subjects. Subtyping of HB(s)Ag was performed by PHI. Among asymptomatic carriers the predominant HB(s)Ag subtype in northeast Asia was adr.In India, ayw predominated in carriers, with the demarcation between adr and ayw occurring west of Burma. In West Africa the only subtype detected was ayw, but in East Africa the majority subtype was adw. The r subtype was found only in Asian populations east of India and in Western Pacific populations. In Papua New Guinea all four subtypes were identified. With one possible exception, the subtypes of HB(s)Ag-positive patients with liver disease reflected the predominant type in each geographic location."}
{"id": "PMID:1084800", "title": "Clinical observations on smallpox: a study of 1233 patients admitted to the Infectious Diseases Hospital, Calcutta, during 1973.", "content": "The paper presents clinical observations on 1 233 persons with smallpox who were admitted to the Infectious Diseases Hospital, Calcutta, in 1973. The disease was of the modified type in 53 patients (4.3%), the ordinary type in 717 (58.2%), the flat type in 249 (20.2%), and the haemorrhagic type in 214 (17.3%). The fatality of these types of smallpox was found to be 5.7%, 26.8%, 88.4%, and 98.1%, respectively, and the overall case fatality was 50.7%. The haemorrhagic type was found mainly among older patients and affected males more often than females. The vaccination status of 1 218 patients was known. Of these, 901 (73.9%) were unvaccinated and had a fatality rate of 53.4%, whereas the 317 (26.1%) vaccinated patients had a fatality rate of 36.5%. Among the 201 haemorrhagic cases, 145 patients were unvaccinated (16.09% of the total number unvaccinated) and 56 (17.67%) had been vaccinated. Of 34 patients vaccinated during the incubation period, 19 (41.1%) died, whereas of 18 patients who had been vaccinated after the onset of fever, but before the appearance of rash, 9 (50%) died.", "contents": "Clinical observations on smallpox: a study of 1233 patients admitted to the Infectious Diseases Hospital, Calcutta, during 1973. The paper presents clinical observations on 1 233 persons with smallpox who were admitted to the Infectious Diseases Hospital, Calcutta, in 1973. The disease was of the modified type in 53 patients (4.3%), the ordinary type in 717 (58.2%), the flat type in 249 (20.2%), and the haemorrhagic type in 214 (17.3%). The fatality of these types of smallpox was found to be 5.7%, 26.8%, 88.4%, and 98.1%, respectively, and the overall case fatality was 50.7%. The haemorrhagic type was found mainly among older patients and affected males more often than females. The vaccination status of 1 218 patients was known. Of these, 901 (73.9%) were unvaccinated and had a fatality rate of 53.4%, whereas the 317 (26.1%) vaccinated patients had a fatality rate of 36.5%. Among the 201 haemorrhagic cases, 145 patients were unvaccinated (16.09% of the total number unvaccinated) and 56 (17.67%) had been vaccinated. Of 34 patients vaccinated during the incubation period, 19 (41.1%) died, whereas of 18 patients who had been vaccinated after the onset of fever, but before the appearance of rash, 9 (50%) died."}
{"id": "PMID:1084801", "title": "The minimum protective level of antibodies in smallpox.", "content": "Blood samples from 57 contacts of 6 smallpox cases were tested for haemagglutination-inhibiting (HI) and neutralizing antibodies. All 6 contacts who subsequently developed smallpox were unvaccinated and had neutralizing antibody titres of 10 or less. However, 6 unvaccinated contacts with similar antibody levels did not develop smallpox. None of the 41 vaccinated contacts, regardless of their antibody level, contracted the disease.", "contents": "The minimum protective level of antibodies in smallpox. Blood samples from 57 contacts of 6 smallpox cases were tested for haemagglutination-inhibiting (HI) and neutralizing antibodies. All 6 contacts who subsequently developed smallpox were unvaccinated and had neutralizing antibody titres of 10 or less. However, 6 unvaccinated contacts with similar antibody levels did not develop smallpox. None of the 41 vaccinated contacts, regardless of their antibody level, contracted the disease."}
{"id": "PMID:1084802", "title": "A simple simulation model of tuberculosis epidemiology for use without large-scale computers.", "content": "A large-scale computer service is not always available in many countries with tuberculosis problems needing epidemiological analysis. To facilitate work in such countries, a simple epidemiological model was made to calculate annual trends in the prevalence and incidence of tuberculosis and its infection, in tuberculosis mortality, and in BCG coverage, using average parameter values not specific for age groups or birth year cohorts. To test its approximation capabilities and limits, the model was applied to epidemiological data from Japan, where sufficient information was available from repeated nation-wide sample surveys and national statistics. The approximation was found to be satisfactory within certain limits. The model is best used with a desk-top computer, but the calculations can be performed with a small calculator or even by hand.", "contents": "A simple simulation model of tuberculosis epidemiology for use without large-scale computers. A large-scale computer service is not always available in many countries with tuberculosis problems needing epidemiological analysis. To facilitate work in such countries, a simple epidemiological model was made to calculate annual trends in the prevalence and incidence of tuberculosis and its infection, in tuberculosis mortality, and in BCG coverage, using average parameter values not specific for age groups or birth year cohorts. To test its approximation capabilities and limits, the model was applied to epidemiological data from Japan, where sufficient information was available from repeated nation-wide sample surveys and national statistics. The approximation was found to be satisfactory within certain limits. The model is best used with a desk-top computer, but the calculations can be performed with a small calculator or even by hand."}
{"id": "PMID:1084803", "title": "Prevalence of Salmonella serotypes in India: a 61-year study.", "content": "During the period 1958-73, 8 027 strains of Salmonella were tested at the National Salmonella and Escherichia Centre: 3 834 strains from man, 3 018 from animals, 839 from sewage and water sources, and 336 of unknown origin. A total of 99 serotypes were identified: 47 from man, 83 from animals, and 35 from sewage and water sources. S. typhi was the commonest serotype in man, followed by S. weltevreden and S. paratyphi A. S. typhimurium was the commonest serotype isolated from animals, followed by S. weltevreden and S. anatum. In sewage and other water sources S. weltevreden was the commonest serotype identified followed by S. typhimurium and S. bareilly. Infection due to S. weltevreden in both man and animals increased considerably after 1970. Out of a total of 99 serotypes isolated from different sources, 13 were isolated from man only, 49 from animals only, 34 from both man and animals, 30 from both animals and water sources, and 22 from man, animals, and water sources.", "contents": "Prevalence of Salmonella serotypes in India: a 61-year study. During the period 1958-73, 8 027 strains of Salmonella were tested at the National Salmonella and Escherichia Centre: 3 834 strains from man, 3 018 from animals, 839 from sewage and water sources, and 336 of unknown origin. A total of 99 serotypes were identified: 47 from man, 83 from animals, and 35 from sewage and water sources. S. typhi was the commonest serotype in man, followed by S. weltevreden and S. paratyphi A. S. typhimurium was the commonest serotype isolated from animals, followed by S. weltevreden and S. anatum. In sewage and other water sources S. weltevreden was the commonest serotype identified followed by S. typhimurium and S. bareilly. Infection due to S. weltevreden in both man and animals increased considerably after 1970. Out of a total of 99 serotypes isolated from different sources, 13 were isolated from man only, 49 from animals only, 34 from both man and animals, 30 from both animals and water sources, and 22 from man, animals, and water sources."}
{"id": "PMID:1084804", "title": "Lactation and reproduction.", "content": "The authors review the literature on the effect of lactation on fertility in the absence of contraception and on the effects of contraceptive measures on lactation. They examine data from several countries on the intervals between births and on the return of menstruation and ovulation after childbirth, comparing lactating with nonlactating women. They conclude that lactation is an inefficient contraceptive for the individual, but that in populations sustained lactation is associated with reduced fertility. Possible physiological mechanisms causing lactation amenorrhoea are discussed. Though much of the literature on the effect of contraceptives on lactation is inadequate, there is general agreement that the estrogen component of hormonal preparations has an adverse effect on lactation, but that progestins alone do not. Many questions remain. Is this effect seen in established lactation, or only in the puerperal period? Is it a direct pharmacological effect, or are pill-users the mothers least motivated to maintain breast-feeding? Does a close relationship exist between hormones given and lactation performance? The authors comment on some of the technical deficiencies of previous studies in this field and discuss practical possibilities of, and limitations to, obtaining adequate scientific information in the future.", "contents": "Lactation and reproduction. The authors review the literature on the effect of lactation on fertility in the absence of contraception and on the effects of contraceptive measures on lactation. They examine data from several countries on the intervals between births and on the return of menstruation and ovulation after childbirth, comparing lactating with nonlactating women. They conclude that lactation is an inefficient contraceptive for the individual, but that in populations sustained lactation is associated with reduced fertility. Possible physiological mechanisms causing lactation amenorrhoea are discussed. Though much of the literature on the effect of contraceptives on lactation is inadequate, there is general agreement that the estrogen component of hormonal preparations has an adverse effect on lactation, but that progestins alone do not. Many questions remain. Is this effect seen in established lactation, or only in the puerperal period? Is it a direct pharmacological effect, or are pill-users the mothers least motivated to maintain breast-feeding? Does a close relationship exist between hormones given and lactation performance? The authors comment on some of the technical deficiencies of previous studies in this field and discuss practical possibilities of, and limitations to, obtaining adequate scientific information in the future."}
{"id": "PMID:1084805", "title": "Testing the susceptibility of Simulium damnosum (Diptera: Simuliidae) to insecticides in the laboratory and in the field.", "content": "There are several methods for measuring the susceptibility of blackfly larvae to insecticides. In 1970 the provisional method suggested by the World Health Organization was used with a view to establishing baseline data on the susceptibility of S. damnosum larvae to insecticides; this method was found to be unsuitable. Thereafter, attempts were made to develop a reliable susceptibility testing method for S. damnosum. This report describes the method that yielded the best results in the laboratory; a second method suitable for use in the field is also described. The results from several replicates of such tests gave reasonably straight log-dosage-probit-mortality curves from which values of LC(50) could be calculated. The overall control mortality in the laboratory series of tests was about 9% and that in the field series was about 4%.", "contents": "Testing the susceptibility of Simulium damnosum (Diptera: Simuliidae) to insecticides in the laboratory and in the field. There are several methods for measuring the susceptibility of blackfly larvae to insecticides. In 1970 the provisional method suggested by the World Health Organization was used with a view to establishing baseline data on the susceptibility of S. damnosum larvae to insecticides; this method was found to be unsuitable. Thereafter, attempts were made to develop a reliable susceptibility testing method for S. damnosum. This report describes the method that yielded the best results in the laboratory; a second method suitable for use in the field is also described. The results from several replicates of such tests gave reasonably straight log-dosage-probit-mortality curves from which values of LC(50) could be calculated. The overall control mortality in the laboratory series of tests was about 9% and that in the field series was about 4%."}
{"id": "PMID:1084806", "title": "The use of psychotropic drugs in developing countries.", "content": "Psychotropic drug therapy combined with other forms of treatment provides an effective means for the control and treatment of a number of mental disorders. In developing countries a wide range of health workers must be prepared to use psychotropic drugs if there is to be a significant improvement in mental health care. A number of problems are involved: not all mental disorders respond to drug treatment; the range of available drugs is very wide; side effects are relatively common; patients may not take prescribed drugs regularly; and there are dangers of overuse, abuse, and overdose. Some of the problems could be overcome by: (a) focusing on a limited number of conditions of public health importance; (b) establishing a clear policy as to which drugs should be available at various points in the health service and limiting the range of such drugs; (c) adopting a more flexible system of task sharing in psychotropic drug therapy; (d) coordinating training programmes; and (e) setting up a central policy body concerned with mental health in health ministries.", "contents": "The use of psychotropic drugs in developing countries. Psychotropic drug therapy combined with other forms of treatment provides an effective means for the control and treatment of a number of mental disorders. In developing countries a wide range of health workers must be prepared to use psychotropic drugs if there is to be a significant improvement in mental health care. A number of problems are involved: not all mental disorders respond to drug treatment; the range of available drugs is very wide; side effects are relatively common; patients may not take prescribed drugs regularly; and there are dangers of overuse, abuse, and overdose. Some of the problems could be overcome by: (a) focusing on a limited number of conditions of public health importance; (b) establishing a clear policy as to which drugs should be available at various points in the health service and limiting the range of such drugs; (c) adopting a more flexible system of task sharing in psychotropic drug therapy; (d) coordinating training programmes; and (e) setting up a central policy body concerned with mental health in health ministries."}
{"id": "PMID:1084807", "title": "Evaluation of the impact of various diseases on mortality.", "content": "The scarcity of resources within the health sector creates serious allocation problems. If it were legitimate to allocate the available means to the various diseases according to their impact on the length of human life, how should this impact be evaluated? That is the purpose of this study, which compares and evaluates methods for measuring the importance of various diseases from the point of view of their lethal effects and significance for life expectancy.THREE METHODS OF EVALUATION HAVE BEEN USED: the percentage of all deaths due to the disease (method A); the gain in life expectancy that would occur if the disease were eradicated as a cause of death (method B); and the change in the ratio between productive and nonproductive groups that would result from eliminating the disease as a cause of death (method C). The analyses are based on the total number of deaths in Denmark in 1969 and 1971. According to (A), one-third of all deaths was caused by heart diseases, one-fourth by cancer, and one-tenth by stroke. The results of method (B) were in agreement with those of method (A); the greatest gain in life expectancy was obtained by eliminating cardiac diseases; cancer ranked second, and accidents third for men and fourth for women. Method (C) yielded contrasting results. The result of eliminating most diseases would be a decrease in the proportion of persons of productive age. This was most distinct for cardiac diseases. The most important exception to this rule was accidents: if these were eliminated, the distribution of the population in the productive and nonproductive age groups would remain stable.", "contents": "Evaluation of the impact of various diseases on mortality. The scarcity of resources within the health sector creates serious allocation problems. If it were legitimate to allocate the available means to the various diseases according to their impact on the length of human life, how should this impact be evaluated? That is the purpose of this study, which compares and evaluates methods for measuring the importance of various diseases from the point of view of their lethal effects and significance for life expectancy.THREE METHODS OF EVALUATION HAVE BEEN USED: the percentage of all deaths due to the disease (method A); the gain in life expectancy that would occur if the disease were eradicated as a cause of death (method B); and the change in the ratio between productive and nonproductive groups that would result from eliminating the disease as a cause of death (method C). The analyses are based on the total number of deaths in Denmark in 1969 and 1971. According to (A), one-third of all deaths was caused by heart diseases, one-fourth by cancer, and one-tenth by stroke. The results of method (B) were in agreement with those of method (A); the greatest gain in life expectancy was obtained by eliminating cardiac diseases; cancer ranked second, and accidents third for men and fourth for women. Method (C) yielded contrasting results. The result of eliminating most diseases would be a decrease in the proportion of persons of productive age. This was most distinct for cardiac diseases. The most important exception to this rule was accidents: if these were eliminated, the distribution of the population in the productive and nonproductive age groups would remain stable."}
{"id": "PMID:1084808", "title": "Respiratory virus disease in Malaysian children: a serological study.", "content": "Paired sera from 101 Malaysian children aged up to 10 years and suffering from respiratory illnesses were examined serologically for evidence of respiratory viral infections. Of these children, 32.6% showed rising antibody titres for one or more of the test agents. Respiratory syncytial virus appeared to be the main respiratory pathogen involved, followed by Mycoplasma pneumoniae, parainfluenza viruses, adenoviruses, and influenza A virus. These findings are generally similar to those reported by others in temperate and tropical countries.", "contents": "Respiratory virus disease in Malaysian children: a serological study. Paired sera from 101 Malaysian children aged up to 10 years and suffering from respiratory illnesses were examined serologically for evidence of respiratory viral infections. Of these children, 32.6% showed rising antibody titres for one or more of the test agents. Respiratory syncytial virus appeared to be the main respiratory pathogen involved, followed by Mycoplasma pneumoniae, parainfluenza viruses, adenoviruses, and influenza A virus. These findings are generally similar to those reported by others in temperate and tropical countries."}
{"id": "PMID:1084809", "title": "Abolition of ischemic response to atrial pacing following aortocoronary bypass surgery.", "content": "To determine the effect of successful aortocoronary bypass surgery (ACBS) on left ventricular (LV) function, the ischemic response to right atrial pacing (RAP) was studied in 22 angina patients before and 3.8 +/- 1.1 months after surgery. All patients were free of angina after ACBS and had at least one patent graft. Before ACBS, RAP induced angina in 15 patients (Group 1) but not in 7 patients (Group 2). After ACBS, no patient had angina with RAP despite the increased maximum rate of pacing. Post-pacing LV end-diastolic pressure (LVEDP) after ACBS decreased in Group 1 from 25 +/- 6 to 15 +/- 6 mm Hg (P less than 0.01), but not in Group 2. Changes in ejection fraction, cardiac output, resting LVEDP, or LVEDP after LV angiography were not significant in either group and were therefore not useful in evaluating the result of ACBS. However the ischemic response to right atrial pacing was abolished by successful aortocoronary bypass surgery, suggesting improved myocardial perfusion during stress.", "contents": "Abolition of ischemic response to atrial pacing following aortocoronary bypass surgery. To determine the effect of successful aortocoronary bypass surgery (ACBS) on left ventricular (LV) function, the ischemic response to right atrial pacing (RAP) was studied in 22 angina patients before and 3.8 +/- 1.1 months after surgery. All patients were free of angina after ACBS and had at least one patent graft. Before ACBS, RAP induced angina in 15 patients (Group 1) but not in 7 patients (Group 2). After ACBS, no patient had angina with RAP despite the increased maximum rate of pacing. Post-pacing LV end-diastolic pressure (LVEDP) after ACBS decreased in Group 1 from 25 +/- 6 to 15 +/- 6 mm Hg (P less than 0.01), but not in Group 2. Changes in ejection fraction, cardiac output, resting LVEDP, or LVEDP after LV angiography were not significant in either group and were therefore not useful in evaluating the result of ACBS. However the ischemic response to right atrial pacing was abolished by successful aortocoronary bypass surgery, suggesting improved myocardial perfusion during stress."}
{"id": "PMID:1084810", "title": "Continuous rapid atrial pacing to control recurrent or sustained supraventricular tachycardias following open heart surgery.", "content": "A technique is described to control recurrent or sustained supraventricular tachycardia associated with rapid ventricular rates following open heart surgery. The technique utilizes a pair of temporarily implanted atrial epicardial wire electrodes to pace the heart. In one group of patients with recurrent atrial flutter and 2:1 A-V conduction, continuous rapid atrial pacing at 450 beats/min produced and sustained atrial fibrillation. The ventricular response rate immediately slowed when compared to that during atrial flutter, and if further slowing was required, it was easily accomplished by the administration of digitalis. Another group of patients with different arrhythmias (recurrent paroxysmal atrial tachycardia, sustained ectopic atrial tachycardia, or sinus rhythm with premature atrial beats which precipitated runs of atrial fibrillation) was treated with continuous rapid atrial pacing to produce 2:1 A-V block. In all instances, the continuous rapid atrial pacing suppressed the supraventricular tachycardia and maintained the ventricular response rate in a therapeutically desirable range. It was demonstrated that the technique is safe, effective, and reliable.", "contents": "Continuous rapid atrial pacing to control recurrent or sustained supraventricular tachycardias following open heart surgery. A technique is described to control recurrent or sustained supraventricular tachycardia associated with rapid ventricular rates following open heart surgery. The technique utilizes a pair of temporarily implanted atrial epicardial wire electrodes to pace the heart. In one group of patients with recurrent atrial flutter and 2:1 A-V conduction, continuous rapid atrial pacing at 450 beats/min produced and sustained atrial fibrillation. The ventricular response rate immediately slowed when compared to that during atrial flutter, and if further slowing was required, it was easily accomplished by the administration of digitalis. Another group of patients with different arrhythmias (recurrent paroxysmal atrial tachycardia, sustained ectopic atrial tachycardia, or sinus rhythm with premature atrial beats which precipitated runs of atrial fibrillation) was treated with continuous rapid atrial pacing to produce 2:1 A-V block. In all instances, the continuous rapid atrial pacing suppressed the supraventricular tachycardia and maintained the ventricular response rate in a therapeutically desirable range. It was demonstrated that the technique is safe, effective, and reliable."}
{"id": "PMID:1084811", "title": "Liver disease--a prominent cause of serum IgE elevation.", "content": "Serum IgE concentrations were elevated in thirty-seven out of sixty-seven patients (55%) with acute or chronic liver disease of widely differing aetiology. The mean IgE concentrations in these patients showed an eight-fold increase above that observed in control subjects. Increased IgE levels in patients with liver disease occurred in the absence of eosinophilia, clinical evidence of atopy or other known causes of IgE elevation. No IgE-containing plasma cells were detected in the liver biopsies from thirty-two of the sixty-seven patients tested. Peripheral blood T cells were significantly decreased from normal in the patients with liver disease, but no correlation emerged between serum IgE levels and absolute peripheral blood T-cell numbers. These findings emphasize the importance of liver disease as a significant cause of serum IgE elevation.", "contents": "Liver disease--a prominent cause of serum IgE elevation. Serum IgE concentrations were elevated in thirty-seven out of sixty-seven patients (55%) with acute or chronic liver disease of widely differing aetiology. The mean IgE concentrations in these patients showed an eight-fold increase above that observed in control subjects. Increased IgE levels in patients with liver disease occurred in the absence of eosinophilia, clinical evidence of atopy or other known causes of IgE elevation. No IgE-containing plasma cells were detected in the liver biopsies from thirty-two of the sixty-seven patients tested. Peripheral blood T cells were significantly decreased from normal in the patients with liver disease, but no correlation emerged between serum IgE levels and absolute peripheral blood T-cell numbers. These findings emphasize the importance of liver disease as a significant cause of serum IgE elevation."}
{"id": "PMID:1084812", "title": "T and B lymphocytes in South American pemphigus foliaceus.", "content": "T and B lymphocytes were quantified in peripheral blood of thirty patients with South American pemphigus foliaceus according to their ability to form rosettes with sheep erythrocytes (E) or sheep erythrocytes sensitized with antibody an complement (EAC). When compared with the counts obtained from thirty normal subjects, a decrease was found in the total T-lymphocyte count (32-33+/-7-7 versus 46-7+/-8-7) and in the T functional lymphocyte count as detected by the active rosette test. The mean percentage of B lymphocytes within the total number of lymphocytes was not significantly different from that of normal subjects (24-0 +/- 8-2 versus 25-3+/-8-0). Lymph node sections from three pemphigus patients examined for E or EAC adherence showed depletion of T cells in the paracortical areas. The low percentage of E cells in the peripheral blood and the depletion of E cells in paracortical areas of lymph nodes from patients with South American pemphigus foliaceus may reflect an impaired cellular immunity.", "contents": "T and B lymphocytes in South American pemphigus foliaceus. T and B lymphocytes were quantified in peripheral blood of thirty patients with South American pemphigus foliaceus according to their ability to form rosettes with sheep erythrocytes (E) or sheep erythrocytes sensitized with antibody an complement (EAC). When compared with the counts obtained from thirty normal subjects, a decrease was found in the total T-lymphocyte count (32-33+/-7-7 versus 46-7+/-8-7) and in the T functional lymphocyte count as detected by the active rosette test. The mean percentage of B lymphocytes within the total number of lymphocytes was not significantly different from that of normal subjects (24-0 +/- 8-2 versus 25-3+/-8-0). Lymph node sections from three pemphigus patients examined for E or EAC adherence showed depletion of T cells in the paracortical areas. The low percentage of E cells in the peripheral blood and the depletion of E cells in paracortical areas of lymph nodes from patients with South American pemphigus foliaceus may reflect an impaired cellular immunity."}
{"id": "PMID:1084813", "title": "Stimulation of lymphocytes by antigen in microplate cultures; absence of an effect of transfer factor in vitro.", "content": "The best conditions for the optimum stimulation of human leucocytes by antigens, including protein purified derivative (PPD), streptokinase-streptodornase varidase (SKSD) and tetanus toxoid have been studied in microplate cultures. The leucocytes of each donor have their own characteristic response to antigen which depends on the culturing conditions such as antigen concentrations, cell concentrations and the time of measuring the rate of DNA synthesis. Thus, no conditions provide a universal optimum for antigen stimulation in vitro. Leucocyte dialysates, i.e. potential transfer factors, have been prepared from donors, who between them are strongly positive to the antigens PPD, SKSD, tetanus toxoid, diphtheria toxoid and Keyhole limpet haemocyanin. In contrast to some previous reports these leucocyte dialysates had no effect on the thymidine incorporation by leucocytes grown in the presence of these antigens. It is suggested that the selection of optimal conditions for the response to antigen may have obscured the effect of any non-specific enhancement of reactivity b lyeucocyte dialysates.", "contents": "Stimulation of lymphocytes by antigen in microplate cultures; absence of an effect of transfer factor in vitro. The best conditions for the optimum stimulation of human leucocytes by antigens, including protein purified derivative (PPD), streptokinase-streptodornase varidase (SKSD) and tetanus toxoid have been studied in microplate cultures. The leucocytes of each donor have their own characteristic response to antigen which depends on the culturing conditions such as antigen concentrations, cell concentrations and the time of measuring the rate of DNA synthesis. Thus, no conditions provide a universal optimum for antigen stimulation in vitro. Leucocyte dialysates, i.e. potential transfer factors, have been prepared from donors, who between them are strongly positive to the antigens PPD, SKSD, tetanus toxoid, diphtheria toxoid and Keyhole limpet haemocyanin. In contrast to some previous reports these leucocyte dialysates had no effect on the thymidine incorporation by leucocytes grown in the presence of these antigens. It is suggested that the selection of optimal conditions for the response to antigen may have obscured the effect of any non-specific enhancement of reactivity b lyeucocyte dialysates."}
{"id": "PMID:1084814", "title": "The effect of cyclophosphamide and irradiation on cells which suppress contact sensitivity in the mouse.", "content": "Contact sensitivity was produced in mice by painting the skin with picryl chloride and was assessed by the increase in ear thickness following local challenge. Contact sensitivity was passively transferred by immune lymph node and spleen cells taken at 4 days. The mice were then challenged immediately and the reactions read at 24 and 48 hr. Immune lymph node and spleen cells taken at day 8 virtually fail to transfer. Experiment showed that they contain cells which suppress passive transfer. These are demonstrated by mixing approximately equal numbers of 4-day cells, which transfer contact sensitivity, and cells taken at later times and injecting them intravenously into recipients. These 'suppressor cells' can be demonstrated by day 6 and are still present at day 11 after immunization. The precursors of the suppressor cells are sensitive to cyclophosphamide. Irradiation of immune mice 2 days before taking cells also selectively inactivates the suppressor cells. When mice are pretreated with cyclophosphamide before immunization or irradiated 2 days before transfer, the lymph node and spleen cells taken on day 9 after immunization transfer contact sensitivity. In contrast the same number of cells from untreated mice were inactive. This suggests that the cells which mediate passive transfer or their precursors may occur in an inhibited form in lymph nodes and spleen at later times after immunization. These suppressor cells in immune mice differ from the T suppressor cells produced by the injection of picryl sulphonic acid--an agent which causes unresponsiveness: (1) the precursors of the T suppressor cells resist cyclophosphamide; (2) the T suppressor cells are found in mice treated so as to produce unresponsiveness while the other type of suppressor cells occurs in mice immunized for contact sensitivity. However, both types of suppressor cells are selectively inactivated by irradiation as compared with the cells which mediate contact sensitivity and both are able to act on the effector stage of contact sensitivity.", "contents": "The effect of cyclophosphamide and irradiation on cells which suppress contact sensitivity in the mouse. Contact sensitivity was produced in mice by painting the skin with picryl chloride and was assessed by the increase in ear thickness following local challenge. Contact sensitivity was passively transferred by immune lymph node and spleen cells taken at 4 days. The mice were then challenged immediately and the reactions read at 24 and 48 hr. Immune lymph node and spleen cells taken at day 8 virtually fail to transfer. Experiment showed that they contain cells which suppress passive transfer. These are demonstrated by mixing approximately equal numbers of 4-day cells, which transfer contact sensitivity, and cells taken at later times and injecting them intravenously into recipients. These 'suppressor cells' can be demonstrated by day 6 and are still present at day 11 after immunization. The precursors of the suppressor cells are sensitive to cyclophosphamide. Irradiation of immune mice 2 days before taking cells also selectively inactivates the suppressor cells. When mice are pretreated with cyclophosphamide before immunization or irradiated 2 days before transfer, the lymph node and spleen cells taken on day 9 after immunization transfer contact sensitivity. In contrast the same number of cells from untreated mice were inactive. This suggests that the cells which mediate passive transfer or their precursors may occur in an inhibited form in lymph nodes and spleen at later times after immunization. These suppressor cells in immune mice differ from the T suppressor cells produced by the injection of picryl sulphonic acid--an agent which causes unresponsiveness: (1) the precursors of the T suppressor cells resist cyclophosphamide; (2) the T suppressor cells are found in mice treated so as to produce unresponsiveness while the other type of suppressor cells occurs in mice immunized for contact sensitivity. However, both types of suppressor cells are selectively inactivated by irradiation as compared with the cells which mediate contact sensitivity and both are able to act on the effector stage of contact sensitivity."}
{"id": "PMID:1084815", "title": "Antibodies for T lymphocytes in systemic lupus erythematosus.", "content": "Sera of twenty-four patients with systemic lupus erythematosus were evaluated for antibodies cytotoxic for autologous lymphocytes. Such antibodies were domenstrable in twenty-two of these sera, whereas only one out of twenty patients with other connective tissue or lymphoproliferative disease showed a positive test. The antibodies remained detectable even when the patients went into remission. Sera containing the lymphocytotoxic antibodies were tested on cell fractions enriched for T or B lymphocytes. Primarily T lumphocytes wree affected by these antibodies. The presence of antibodies cytotoxic for T lymphocytes corresponded with a deficit of circulating T lymphocytes observed in most of our patients with systemic lupus erythematosys.", "contents": "Antibodies for T lymphocytes in systemic lupus erythematosus. Sera of twenty-four patients with systemic lupus erythematosus were evaluated for antibodies cytotoxic for autologous lymphocytes. Such antibodies were domenstrable in twenty-two of these sera, whereas only one out of twenty patients with other connective tissue or lymphoproliferative disease showed a positive test. The antibodies remained detectable even when the patients went into remission. Sera containing the lymphocytotoxic antibodies were tested on cell fractions enriched for T or B lymphocytes. Primarily T lumphocytes wree affected by these antibodies. The presence of antibodies cytotoxic for T lymphocytes corresponded with a deficit of circulating T lymphocytes observed in most of our patients with systemic lupus erythematosys."}
{"id": "PMID:1084816", "title": "Soluble factors in immune sera of mice. II. Reversible loss of surface immunoglobulin induced in vitro.", "content": "Serum collected from BALB/c mice at different time intervals during primary immunization induces in vitro a decrease of Ig+ cells in short-term cultures of normal spleen cells. The decrease was shown to be caused by loss of surface immunoglobulin (Ig) for the following reasons: no cell loss was detected which coulc account for it; the number of Ig+ cells returned to normal levels when the serum was removed and the cells were cultured further in fresh medium. The serum activity was recovered in a fraction with molecular weight less than 10,000 Daltons. The factor was active at 4 degrees C and on cells treated with high doses of Con A. Only a portion (25-30%) of the Ig+ cells are affected by this factor since after the initial decrease no further changes were observed during the 7 hr culture period. No changes in the distrubution of surface Ig was detected by fluorescent techniques on the remaining Ig+ cells. This factor was detected as early as 6 hr after immunization but its concentration was found 4-fold higher on the 7th day.", "contents": "Soluble factors in immune sera of mice. II. Reversible loss of surface immunoglobulin induced in vitro. Serum collected from BALB/c mice at different time intervals during primary immunization induces in vitro a decrease of Ig+ cells in short-term cultures of normal spleen cells. The decrease was shown to be caused by loss of surface immunoglobulin (Ig) for the following reasons: no cell loss was detected which coulc account for it; the number of Ig+ cells returned to normal levels when the serum was removed and the cells were cultured further in fresh medium. The serum activity was recovered in a fraction with molecular weight less than 10,000 Daltons. The factor was active at 4 degrees C and on cells treated with high doses of Con A. Only a portion (25-30%) of the Ig+ cells are affected by this factor since after the initial decrease no further changes were observed during the 7 hr culture period. No changes in the distrubution of surface Ig was detected by fluorescent techniques on the remaining Ig+ cells. This factor was detected as early as 6 hr after immunization but its concentration was found 4-fold higher on the 7th day."}
{"id": "PMID:1084817", "title": "Suppressed hetergeneous antinuclear antibody response in lymphomabearing NZB/NZW mice.", "content": "A long-term study of cyclophophamide-induced suppression of auto-immune disease in NZB/NZW mice demonstrated the oncogenic potential of this alkylating agent. In seven of ten mice dying with lymphomas, retrospective examination of serial tests for heterogeneous ANA showed terminal decreases of titres or reversion of tests to negative. Suppressed titres were found in one of seven mice dying with other tumours and in four of twenty-nine mice succumbing to renal disease-vasculitis. Transplantation studies confirmed the association between growing lymphoma tissue and decreasing ANA titres. In the immunosuppressed host with connective tissue disease, the loss of certain auto-antibodies may signal development of a lymphoreticular neoplasm. Tumour-induced impairment of auto-immune responsiveness may reflect the loss of specific ANA that fulfil a protective role and inhibit neoplastic growth.", "contents": "Suppressed hetergeneous antinuclear antibody response in lymphomabearing NZB/NZW mice. A long-term study of cyclophophamide-induced suppression of auto-immune disease in NZB/NZW mice demonstrated the oncogenic potential of this alkylating agent. In seven of ten mice dying with lymphomas, retrospective examination of serial tests for heterogeneous ANA showed terminal decreases of titres or reversion of tests to negative. Suppressed titres were found in one of seven mice dying with other tumours and in four of twenty-nine mice succumbing to renal disease-vasculitis. Transplantation studies confirmed the association between growing lymphoma tissue and decreasing ANA titres. In the immunosuppressed host with connective tissue disease, the loss of certain auto-antibodies may signal development of a lymphoreticular neoplasm. Tumour-induced impairment of auto-immune responsiveness may reflect the loss of specific ANA that fulfil a protective role and inhibit neoplastic growth."}
{"id": "PMID:1084818", "title": "Mechanisms of corticosteroid action on lymphocyte subpopulations. II. Differential effects of in vivo hydrocortisone, prednisone and dexamethasone on in vitro expression of lymphocyte function.", "content": "The present study was undertaken to determine what, if any, differential effect various commonly used corticosteroid preparations had on the numbers and specific functions of lymphocyte subpopulations when these agents were administered in equivalent pharmacological dosages. Normal volunteers received a single dose of either 320 mg of hydrocortisone intravenously, 80 mg of prednisone orally, or 12 mg of dexamethasone orally. There was a marked lymphocytopenia and monocytopenia maximal 4-6 hr following administration of all three corticosteroid preparations with almost identical kinetics and degree of fall in total cell numbers as well as proportions of thymus-derived and bone marrow-derived lymphocytes. Hydrocortisone and prednisone caused only a slight suppression of phytohaemagglutiinin (PHA) induced lymphocyte blastogensis which could be reversed at supra-optimal concentrations of PHA. On the contrary, dexamethasone administration casued a marked suppression of PHA responses which was not reversed by supra-optimal PHA stimulation. In addition, hydrocortisone and prednisone administration did not suppress non-specific PHA-induced cellular cytotoxcity, while dexamethasone caused a marked suppression (P less than 0.001) of cytotoxicity. These studies show that although equivalent anti-inflammatory doses of these three corticosteroid preparations cause almost identical suppression of the numbers of circulating lymphocyte populations, they have a differential effect of the numbers of circulating lymphocyte populations, they have a differential effect on a certain in vitro functional correlates of cell-mediated immunity.", "contents": "Mechanisms of corticosteroid action on lymphocyte subpopulations. II. Differential effects of in vivo hydrocortisone, prednisone and dexamethasone on in vitro expression of lymphocyte function. The present study was undertaken to determine what, if any, differential effect various commonly used corticosteroid preparations had on the numbers and specific functions of lymphocyte subpopulations when these agents were administered in equivalent pharmacological dosages. Normal volunteers received a single dose of either 320 mg of hydrocortisone intravenously, 80 mg of prednisone orally, or 12 mg of dexamethasone orally. There was a marked lymphocytopenia and monocytopenia maximal 4-6 hr following administration of all three corticosteroid preparations with almost identical kinetics and degree of fall in total cell numbers as well as proportions of thymus-derived and bone marrow-derived lymphocytes. Hydrocortisone and prednisone caused only a slight suppression of phytohaemagglutiinin (PHA) induced lymphocyte blastogensis which could be reversed at supra-optimal concentrations of PHA. On the contrary, dexamethasone administration casued a marked suppression of PHA responses which was not reversed by supra-optimal PHA stimulation. In addition, hydrocortisone and prednisone administration did not suppress non-specific PHA-induced cellular cytotoxcity, while dexamethasone caused a marked suppression (P less than 0.001) of cytotoxicity. These studies show that although equivalent anti-inflammatory doses of these three corticosteroid preparations cause almost identical suppression of the numbers of circulating lymphocyte populations, they have a differential effect of the numbers of circulating lymphocyte populations, they have a differential effect on a certain in vitro functional correlates of cell-mediated immunity."}
{"id": "PMID:1084819", "title": "Respiratory involvement in systemic lupus erythematosus. A clinical and immunological study.", "content": "Thirty patients fulfilling conventional criteria for systemic lupus erythematosus and who presented with extensive pleural and pulmonary involvement were studied retrospectively. Four overlapping patterns of respiratory disease were identified and observations were made on their clinical presentation, radiographic abnormalities and response to treatment. A low incidence of severe renal disease was found in this series of patients and this was in keeping with the general finding of low serum binding using native DNA in a globulin Farr-binding technique (greater than 20% binding in only 4/21 (19%) of the series) and normal or elevated serum complement (C3) levels. Precipitating antibody detected by double diffusion and counter-current immunoelectrophoresis and probably reacting in most cases with single-stranded DNA was, however, detected in 66% of pretreatment serum samples tested. This evidence supports the idea that different types of anti-nuclear antibody may be associated with different clinical manifestations seen within a group of patients who broadly fulfil the criteria for SLE.", "contents": "Respiratory involvement in systemic lupus erythematosus. A clinical and immunological study. Thirty patients fulfilling conventional criteria for systemic lupus erythematosus and who presented with extensive pleural and pulmonary involvement were studied retrospectively. Four overlapping patterns of respiratory disease were identified and observations were made on their clinical presentation, radiographic abnormalities and response to treatment. A low incidence of severe renal disease was found in this series of patients and this was in keeping with the general finding of low serum binding using native DNA in a globulin Farr-binding technique (greater than 20% binding in only 4/21 (19%) of the series) and normal or elevated serum complement (C3) levels. Precipitating antibody detected by double diffusion and counter-current immunoelectrophoresis and probably reacting in most cases with single-stranded DNA was, however, detected in 66% of pretreatment serum samples tested. This evidence supports the idea that different types of anti-nuclear antibody may be associated with different clinical manifestations seen within a group of patients who broadly fulfil the criteria for SLE."}
{"id": "PMID:1084820", "title": "Measles antibodies and autoantibodies in autoimmune disorders.", "content": "Measles CF antibodies have been examined in the sera of patients with a variety of clinical disorders associated with the production of autoantibodies. Previous reports of high-titre reactions in DLE and chronic active hepatitis have been confirmed, the titres in the latter disorder being particularly elevated. Mean antibody titres to measles in patients with rheumatoid arthritis were significantly lower than in matched controls, and an inverse correlation between measles antibody levels and serum globulin levels was found. Measles antibody titres in patients with myasthenia gravis and primary biliary cirrhosis did not differ significantly from those found in controls. However, subdivision of patients with rheumatoid arthritis, myasthenia gravis and primary biliary cirrhosis showed that the presence of anti-nuclear antibody (ANA) was associated with significantly increased measles antibody levels compared with the ANA-negative sera. The presence of gastric parietal cell antibody or thyroid microsomal antibody did not appear to be associated with increased measles antibody levels, whether or not they occurred in association with previous anaemia or thyroid disease. Possible explanations for these findings in terms of immune complex formation and immune hyper-reactivity are discussed.", "contents": "Measles antibodies and autoantibodies in autoimmune disorders. Measles CF antibodies have been examined in the sera of patients with a variety of clinical disorders associated with the production of autoantibodies. Previous reports of high-titre reactions in DLE and chronic active hepatitis have been confirmed, the titres in the latter disorder being particularly elevated. Mean antibody titres to measles in patients with rheumatoid arthritis were significantly lower than in matched controls, and an inverse correlation between measles antibody levels and serum globulin levels was found. Measles antibody titres in patients with myasthenia gravis and primary biliary cirrhosis did not differ significantly from those found in controls. However, subdivision of patients with rheumatoid arthritis, myasthenia gravis and primary biliary cirrhosis showed that the presence of anti-nuclear antibody (ANA) was associated with significantly increased measles antibody levels compared with the ANA-negative sera. The presence of gastric parietal cell antibody or thyroid microsomal antibody did not appear to be associated with increased measles antibody levels, whether or not they occurred in association with previous anaemia or thyroid disease. Possible explanations for these findings in terms of immune complex formation and immune hyper-reactivity are discussed."}
{"id": "PMID:1084821", "title": "The effect of Corynebacterium parvum on the humoral and cellular immune systems in patients with breast cancer.", "content": "Corynebacterium parvum, a Gram-positive anaerobic bacillus thought to be a strong immunological stimulant, has been shown to decrease tumour growth and prolong survival in patients with metastatic disease. Study of the effect of a single injection of a strain of C. parvum (CN. 6134) in six patients with stage IV metastatic breast cancer is reported. Results of laboratory tests to judge the physical and immunological effects of the drug infusion 24 hr post-treatment and weekly thereafter for 3 weeks are evaluated. Within 24 hr after C. parvum administration, most patients experienced fever and nausea. Blood counts and differential counts exhibited increased values 24 hr after treatment with a strong shift to the left. Lymphocyte and monocyte counts were greatly depressed at 24 hr. T-cell numbers in peripheral blood did not appear to be altered, but the picture with regard to B cells was less clear. Normal count was recovered by day 8. It appears that intravenous administration of C. parvum produces a temporary marked immunological depression which returns to essentially normal values in 8 days. The return to normal may be accompanied by resolution of the endotoxin-like syndrome of side-effects. Further study of patients receiving this therapeutic agent is important to detect enhancement of the anti-tumour immunological response precipitated.", "contents": "The effect of Corynebacterium parvum on the humoral and cellular immune systems in patients with breast cancer. Corynebacterium parvum, a Gram-positive anaerobic bacillus thought to be a strong immunological stimulant, has been shown to decrease tumour growth and prolong survival in patients with metastatic disease. Study of the effect of a single injection of a strain of C. parvum (CN. 6134) in six patients with stage IV metastatic breast cancer is reported. Results of laboratory tests to judge the physical and immunological effects of the drug infusion 24 hr post-treatment and weekly thereafter for 3 weeks are evaluated. Within 24 hr after C. parvum administration, most patients experienced fever and nausea. Blood counts and differential counts exhibited increased values 24 hr after treatment with a strong shift to the left. Lymphocyte and monocyte counts were greatly depressed at 24 hr. T-cell numbers in peripheral blood did not appear to be altered, but the picture with regard to B cells was less clear. Normal count was recovered by day 8. It appears that intravenous administration of C. parvum produces a temporary marked immunological depression which returns to essentially normal values in 8 days. The return to normal may be accompanied by resolution of the endotoxin-like syndrome of side-effects. Further study of patients receiving this therapeutic agent is important to detect enhancement of the anti-tumour immunological response precipitated."}
{"id": "PMID:1084822", "title": "Depression of the T-lymphocyte response to phytohaemagglutinin by renal cells.", "content": "The lymphocytic infiltrate in the renal parenchyma is a consistent histological feature of pyelonephritis, but the role of the lymphocytes in the immunobiology of pyelonephritis is not known. In this investigation the influence of the local environment on the potential function of T lymphocytes in the kidney was investigated. The experiments have demonstrated that the response of rat lymphocytes to stimulation in vitro with phytohaemagglutinin (PHA) can be entirely ablated by normal kidney cells. Even when the number of kidney cells added to cultures of lymphocytes was less than 2% of the cells present some ablation of T-lymphocyte function could be detected. The biological characteristics of the factor causing ablation of the PHA responsiveness of T lymphocytes were partially characterized and the factor appears to have unique features that differentiate it from lymphocyte chalones and other tissue factors influencing lymphocyte function. The results may explain recent findings where T lymphocytes were found to be the predominant lymphocyte in the inflammatory infiltrate but were not responsive to PHA in vitro.", "contents": "Depression of the T-lymphocyte response to phytohaemagglutinin by renal cells. The lymphocytic infiltrate in the renal parenchyma is a consistent histological feature of pyelonephritis, but the role of the lymphocytes in the immunobiology of pyelonephritis is not known. In this investigation the influence of the local environment on the potential function of T lymphocytes in the kidney was investigated. The experiments have demonstrated that the response of rat lymphocytes to stimulation in vitro with phytohaemagglutinin (PHA) can be entirely ablated by normal kidney cells. Even when the number of kidney cells added to cultures of lymphocytes was less than 2% of the cells present some ablation of T-lymphocyte function could be detected. The biological characteristics of the factor causing ablation of the PHA responsiveness of T lymphocytes were partially characterized and the factor appears to have unique features that differentiate it from lymphocyte chalones and other tissue factors influencing lymphocyte function. The results may explain recent findings where T lymphocytes were found to be the predominant lymphocyte in the inflammatory infiltrate but were not responsive to PHA in vitro."}
{"id": "PMID:1084825", "title": "Intestinal blood loss after a new anti-inflammatory drug, sulindac.", "content": "Sulindac was tested for fecal blood loss in 40 healthy male subjects whose red cells had been labeled with Na2 51 CrO4. Two daily dose levels of 240 mg and 400 mg were compared with 4.8 gm of aspirin and placebo for 2 wk. At day 15, aspirin-induced blood loss was greater than that of both dose levels of sulindac and of placebo (p less than 0.05). There were no significant differences between the two sulindac groups and the placebo group. Aspirin caused more adverse reactions than sulindac, 240 mg (p less than 0.05), 400 mg (p less than 0.05), and placebo (p less than 0.05).", "contents": "Intestinal blood loss after a new anti-inflammatory drug, sulindac. Sulindac was tested for fecal blood loss in 40 healthy male subjects whose red cells had been labeled with Na2 51 CrO4. Two daily dose levels of 240 mg and 400 mg were compared with 4.8 gm of aspirin and placebo for 2 wk. At day 15, aspirin-induced blood loss was greater than that of both dose levels of sulindac and of placebo (p less than 0.05). There were no significant differences between the two sulindac groups and the placebo group. Aspirin caused more adverse reactions than sulindac, 240 mg (p less than 0.05), 400 mg (p less than 0.05), and placebo (p less than 0.05)."}
{"id": "PMID:1084827", "title": "Fiberoptic colonoscopy: complications of colonoscopy and polypectomy.", "content": "Members of the American Society of Colon and Rectal Surgeons were polled regarding complications of colonoscopy and polypectomy. Sixty per cent of the members responded. One hundred sixty-two surgeons were found to be performing colonoscopy, and this paper presents the accumulated data on complications. A total of 20,139 colonoscopies were performed, with 12,746 diagnostic procedures and 7,393 polypectomies. The overall complication rate was 0.4 per cent with diagnostic colonoscopy and 1.8 per cent with polypectomy. Based upon these complications, guidelines in regard to history, preparation, and technique of colonoscopy and polypectomy are discussed.", "contents": "Fiberoptic colonoscopy: complications of colonoscopy and polypectomy. Members of the American Society of Colon and Rectal Surgeons were polled regarding complications of colonoscopy and polypectomy. Sixty per cent of the members responded. One hundred sixty-two surgeons were found to be performing colonoscopy, and this paper presents the accumulated data on complications. A total of 20,139 colonoscopies were performed, with 12,746 diagnostic procedures and 7,393 polypectomies. The overall complication rate was 0.4 per cent with diagnostic colonoscopy and 1.8 per cent with polypectomy. Based upon these complications, guidelines in regard to history, preparation, and technique of colonoscopy and polypectomy are discussed."}
{"id": "PMID:1084831", "title": "Cyproterone-mediated stimulation of delta-aminolevulinic acid synthetase in chick embryo liver cells.", "content": "In cultured chick embryo liver cells, cyproterone and cyproterone acetate, synthetic anti-androgenic steroids, were found to be potent inducers of mitochondrial delta-aminolevulinic acid (ALA) synthetase, the rate-limiting enzyme in the heme biosynthetic pathway. Both steroids produced a detectable increase in enzyme activity at 5 muM and a maximal stimulation, 36-fold for cyproterone and 29-fold for cyproterone acetate, at 55 muM. The dose-response curves of the steroids differed, however, in that cyproterone acetate produced a greater mean stimulation of the enzyme at concentrations less than approximately 25 muM, whereas, at higher concentrations, cyproterone was the more effective inducer. Increased activity of ALA synthetase was not apparent until about 12 hours after the addition of cyproterone, and maximal activity was not achieved until 20-24 hours. The induction of ALA synthetase by these anti-androgens was prevented by actinomycin D, cordycepin, anisomycin, cycloheximide, and puromycin. These results suggest that new RNA and protein synthesis are necessary for enzyme induction. The cyproterone-mediated induction of the enzyme was inhibited 50% by 2 muM heme, the putative physiological inhibitor of ALA synthetase. These antiandrogens, unlike other potent steroid inducers of this enzyme in chick embryo liver, do not possess either a 5beta-pregnane or 5beta-androstane nucleus. The stimulation of hepatic ALA synthetase represents the first example of a direct effect of these steroids on enzyme induction.", "contents": "Cyproterone-mediated stimulation of delta-aminolevulinic acid synthetase in chick embryo liver cells. In cultured chick embryo liver cells, cyproterone and cyproterone acetate, synthetic anti-androgenic steroids, were found to be potent inducers of mitochondrial delta-aminolevulinic acid (ALA) synthetase, the rate-limiting enzyme in the heme biosynthetic pathway. Both steroids produced a detectable increase in enzyme activity at 5 muM and a maximal stimulation, 36-fold for cyproterone and 29-fold for cyproterone acetate, at 55 muM. The dose-response curves of the steroids differed, however, in that cyproterone acetate produced a greater mean stimulation of the enzyme at concentrations less than approximately 25 muM, whereas, at higher concentrations, cyproterone was the more effective inducer. Increased activity of ALA synthetase was not apparent until about 12 hours after the addition of cyproterone, and maximal activity was not achieved until 20-24 hours. The induction of ALA synthetase by these anti-androgens was prevented by actinomycin D, cordycepin, anisomycin, cycloheximide, and puromycin. These results suggest that new RNA and protein synthesis are necessary for enzyme induction. The cyproterone-mediated induction of the enzyme was inhibited 50% by 2 muM heme, the putative physiological inhibitor of ALA synthetase. These antiandrogens, unlike other potent steroid inducers of this enzyme in chick embryo liver, do not possess either a 5beta-pregnane or 5beta-androstane nucleus. The stimulation of hepatic ALA synthetase represents the first example of a direct effect of these steroids on enzyme induction."}
{"id": "PMID:1084835", "title": "[Charge-transfer complexes of hematoporphyrin with pteridine derivatives].", "content": "Charge-transfer complexes of hematoporphyrin with pteridine derivatives (folic acid, folinic acid, methotrexate) were studied in solutions buffered at pH 7.5 - 9 -10. The association constants for folic acid are pH dependent; at pH 7.5 the constant is 1240 wheras at pH 10 it is only 65. The association constant for folinic acid was determined only at pH 7.5 and was found to be 55. The association constants of methotexate are approximately the same at different pH's.", "contents": "[Charge-transfer complexes of hematoporphyrin with pteridine derivatives]. Charge-transfer complexes of hematoporphyrin with pteridine derivatives (folic acid, folinic acid, methotrexate) were studied in solutions buffered at pH 7.5 - 9 -10. The association constants for folic acid are pH dependent; at pH 7.5 the constant is 1240 wheras at pH 10 it is only 65. The association constant for folinic acid was determined only at pH 7.5 and was found to be 55. The association constants of methotexate are approximately the same at different pH's."}
{"id": "PMID:1084836", "title": "Clones of B lymphocytes: their natural selection and expansion.", "content": "The operation of clonal selection for cells of the B-lymphocyte line is discussed with regard to: 1) The clonal repertoire determined by antigen binding to B lymphocytes, which is much larger than that determined by limiting dilution cloning assays. This quantitative difference is interpreted in terms of the multiple shared specificities of each antibody molecule. 2) Multiclonal responses and initial selection by antigen of particular clones (preferential primary selection). 3) Clonal dominance. During an immune response one clone (or a small number of clones) of B cells is preferentially selected and proliferated, apparently at random, from a heterogeneous population of cells capable of responding to the given antigen. Co-dominance of two or more clones simultaneously can be obtained by mixing selected clones. Secreted antibody is seen as playing a role in the establishment of clonal dominance. A model for clonal expansion is presented. The model attempts to explain the generation of memory and antibody secreting cells within each clonal expansion in terms of the ratio of two signals, one for proliferation and one for differentiation. The delivery of these signals is proposed to involve the receptor antibody-antigen interaction for proliferation and a self-recognition site interaction for differentiation.", "contents": "Clones of B lymphocytes: their natural selection and expansion. The operation of clonal selection for cells of the B-lymphocyte line is discussed with regard to: 1) The clonal repertoire determined by antigen binding to B lymphocytes, which is much larger than that determined by limiting dilution cloning assays. This quantitative difference is interpreted in terms of the multiple shared specificities of each antibody molecule. 2) Multiclonal responses and initial selection by antigen of particular clones (preferential primary selection). 3) Clonal dominance. During an immune response one clone (or a small number of clones) of B cells is preferentially selected and proliferated, apparently at random, from a heterogeneous population of cells capable of responding to the given antigen. Co-dominance of two or more clones simultaneously can be obtained by mixing selected clones. Secreted antibody is seen as playing a role in the establishment of clonal dominance. A model for clonal expansion is presented. The model attempts to explain the generation of memory and antibody secreting cells within each clonal expansion in terms of the ratio of two signals, one for proliferation and one for differentiation. The delivery of these signals is proposed to involve the receptor antibody-antigen interaction for proliferation and a self-recognition site interaction for differentiation."}
{"id": "PMID:1084837", "title": "Studies on the mode of action of ecdysterone in adult female Aedes aegypti.", "content": "Injection of ecdysterone into non-blood fed adult female Aedes aegypti results in a marked stimulation of aromatic-L-amino-acid decarboxylase (formerly DOPA-decarboxylase) activity (Schlaeger and Fuchs, 1974a). When the hormone and alpha-amanitin are injected either simultaneously or if the toxin is administered first no inhibition of subsequent enzymatic activity is observed and in fact substantial enhancement occurs. Cordycepin injection along with ecdysterone gives results similar to alpha-amanitin. The inhibitors by themselves elicit a very small increase in DOPA decarboxylase activity compared to saline-injected controls. Conversely, actinomycin D causes severe depression of ecdysterone-mediated DOPA decarboxylase activity as dose cycloheximide and puromycin. We interpret our data to mean that the mRNA for DOPA decarboxylase is already present prior to exposure to ecdysterone. We postulate that the function of the hormone would be to modulate translation of specific pre-formed mRNA's by an unknown mechanism or to induce transcription of specific tRNAs necessary for the initation of translation of selecting existing messengers.", "contents": "Studies on the mode of action of ecdysterone in adult female Aedes aegypti. Injection of ecdysterone into non-blood fed adult female Aedes aegypti results in a marked stimulation of aromatic-L-amino-acid decarboxylase (formerly DOPA-decarboxylase) activity (Schlaeger and Fuchs, 1974a). When the hormone and alpha-amanitin are injected either simultaneously or if the toxin is administered first no inhibition of subsequent enzymatic activity is observed and in fact substantial enhancement occurs. Cordycepin injection along with ecdysterone gives results similar to alpha-amanitin. The inhibitors by themselves elicit a very small increase in DOPA decarboxylase activity compared to saline-injected controls. Conversely, actinomycin D causes severe depression of ecdysterone-mediated DOPA decarboxylase activity as dose cycloheximide and puromycin. We interpret our data to mean that the mRNA for DOPA decarboxylase is already present prior to exposure to ecdysterone. We postulate that the function of the hormone would be to modulate translation of specific pre-formed mRNA's by an unknown mechanism or to induce transcription of specific tRNAs necessary for the initation of translation of selecting existing messengers."}
{"id": "PMID:1084840", "title": "Does lower esophageal sphincter incompetency contribute to esophageal bleeding?", "content": "The purpose of this study is to determine whether lower esophageal sphincter (LES) incompetency is a common occurrence in patients with liver cirrhosis and contributes to the development of variceal bleeding. Resting LES pressure (17.8 +/- 1.1 mm Hg) in 35 patients with cirrhosis was similar to that of our control population (17.3 +/- 2.0 mm Hg). No differences were found among patients with ascites, variceal hemorrhage, or with different degrees of hepatic decompensation. In both patients and control subjects the LES responded with a significant pressure increase to gastric alkalinization. Symptoms and radiological evidence of gastroesophageal reflux were extremely uncommon in patients with liver cirrhosis. Based on these data it is unlikely that acid-pepsin regurgitation is a significant factor in the development of variceal hemorrhage.", "contents": "Does lower esophageal sphincter incompetency contribute to esophageal bleeding? The purpose of this study is to determine whether lower esophageal sphincter (LES) incompetency is a common occurrence in patients with liver cirrhosis and contributes to the development of variceal bleeding. Resting LES pressure (17.8 +/- 1.1 mm Hg) in 35 patients with cirrhosis was similar to that of our control population (17.3 +/- 2.0 mm Hg). No differences were found among patients with ascites, variceal hemorrhage, or with different degrees of hepatic decompensation. In both patients and control subjects the LES responded with a significant pressure increase to gastric alkalinization. Symptoms and radiological evidence of gastroesophageal reflux were extremely uncommon in patients with liver cirrhosis. Based on these data it is unlikely that acid-pepsin regurgitation is a significant factor in the development of variceal hemorrhage."}
{"id": "PMID:1084841", "title": "Indications for surgery in Crohn's disease: analysis of 500 cases.", "content": "Data for 500 patients with Crohn's disease who underwent operations were analyzed (316 patients, 1966 to 1969; 184 patients, 1972 to 1973) by comparison of various anatomic disease locations (clinical pattern): (1) ileocolic, 225 patients; (2) small intestinal, 130 patients; (3) colonic, 127 patients; (4) anorectal, 18 patients. Indications for surgery were tabulated and compared using statistical analysis for the three large patterns. For patients with ileocolic Crohn's disease, the primary surgical indications were internal fistula and abscess, 44%, intestinal obstruction, 35%, and perianal disease, 12%. For patients with Crohn's disease of the small intestine, the primary surgical indications were intestinal obstruction, 55%, and intestinal fistula and abscess, 32%. Patients with colonic Crohn's disease had a significantly more diverse surgical indication, with poor response to medical therapy, 26%, internal fistula and abscess, 23%, toxic megacolon, 20%, and perianal disease, 19%. These values were highly statistically significant (P less than 0.0001) in all instances but one. This study demonstrates that statistically significant differences occur in the surgical indication depending on the location of Crohn's disease. Patients with ileocolic, small intestinal, and colonic involvement have striking differences in clinical course. It is concluded that Crohn's disease is not a homogeneous entity, but should be recognized as having a varying course depending on clinical pattern.", "contents": "Indications for surgery in Crohn's disease: analysis of 500 cases. Data for 500 patients with Crohn's disease who underwent operations were analyzed (316 patients, 1966 to 1969; 184 patients, 1972 to 1973) by comparison of various anatomic disease locations (clinical pattern): (1) ileocolic, 225 patients; (2) small intestinal, 130 patients; (3) colonic, 127 patients; (4) anorectal, 18 patients. Indications for surgery were tabulated and compared using statistical analysis for the three large patterns. For patients with ileocolic Crohn's disease, the primary surgical indications were internal fistula and abscess, 44%, intestinal obstruction, 35%, and perianal disease, 12%. For patients with Crohn's disease of the small intestine, the primary surgical indications were intestinal obstruction, 55%, and intestinal fistula and abscess, 32%. Patients with colonic Crohn's disease had a significantly more diverse surgical indication, with poor response to medical therapy, 26%, internal fistula and abscess, 23%, toxic megacolon, 20%, and perianal disease, 19%. These values were highly statistically significant (P less than 0.0001) in all instances but one. This study demonstrates that statistically significant differences occur in the surgical indication depending on the location of Crohn's disease. Patients with ileocolic, small intestinal, and colonic involvement have striking differences in clinical course. It is concluded that Crohn's disease is not a homogeneous entity, but should be recognized as having a varying course depending on clinical pattern."}
{"id": "PMID:1084842", "title": "Immunological studies in familial primary biliary cirrhosis.", "content": "Tests for cell-mediated immunity and presence of autoantibodies were performed in a mother and daughter with primary biliary cirrhosis. Lymphocytes transformation to phytohemagglutinin, delayed cutaneous response to one or more skin test antigens, and percentage of peripheral T and B lymphocytes were normal in both patients. Although successful in the mother, dinitrochlorbenzene sensitization was not achieved in the daughter. Histocompatibility antigens 1 and 8, elevated levels of IgM, and antibodies to mitochondria, smooth muscle, and skeletal muscle were present in both patients. However, the clinical course was more severe in the daughter who developed portal hypertension with bleeding esophageal varices requiring portacaval anastomosis. Except for intermittent pruritus, the mother has remained asymptomatic.", "contents": "Immunological studies in familial primary biliary cirrhosis. Tests for cell-mediated immunity and presence of autoantibodies were performed in a mother and daughter with primary biliary cirrhosis. Lymphocytes transformation to phytohemagglutinin, delayed cutaneous response to one or more skin test antigens, and percentage of peripheral T and B lymphocytes were normal in both patients. Although successful in the mother, dinitrochlorbenzene sensitization was not achieved in the daughter. Histocompatibility antigens 1 and 8, elevated levels of IgM, and antibodies to mitochondria, smooth muscle, and skeletal muscle were present in both patients. However, the clinical course was more severe in the daughter who developed portal hypertension with bleeding esophageal varices requiring portacaval anastomosis. Except for intermittent pruritus, the mother has remained asymptomatic."}
{"id": "PMID:1084843", "title": "Massive splenic infarction in cirrhosis: report of a case with spontaneous disappearance of hypersplenism.", "content": "A cirrhotic patient with massive splenic infarction is described. Celiac angiography showed normally opacified splenic artery and vein and a markedly enlarged spleen with large avascular zones. Splenic infarction was associated with the spontaneous disappearance of a syndrome of hypersplenism. The spleen was surgically removed. Histological examination showed multiple thromboses of the small arterial and venous vessels. The cause of this infarct remained unclear.", "contents": "Massive splenic infarction in cirrhosis: report of a case with spontaneous disappearance of hypersplenism. A cirrhotic patient with massive splenic infarction is described. Celiac angiography showed normally opacified splenic artery and vein and a markedly enlarged spleen with large avascular zones. Splenic infarction was associated with the spontaneous disappearance of a syndrome of hypersplenism. The spleen was surgically removed. Histological examination showed multiple thromboses of the small arterial and venous vessels. The cause of this infarct remained unclear."}
{"id": "PMID:1084844", "title": "The subpopulations of circulating white blood cells in inflammatory bowel disease.", "content": "A detailed analysis of the species of lymphocytes was carried out in 58 patients with inflammatory bowel disease (IBD). These individuals were further divided into 31 with Crohn's disease (CD) and 27 with ulcerative colitis (UC). There were 13 CD patients with only small bowel involvement called \"regional enteritis\" and 18 who had some degree of colonic involvement called \"ileocolitis\". Similarly, the UC group was subdivided into 9 patients with disease confined to the rectosigmoid area called \"proctosigmoiditis\" and 18 with more extensive involvement called \"universal colitis\". We also studied 13 patients who had undergone previous colectomy and ileostomy and 78 healthy age- and sex-matched controls. Although there was no increase in the absolute number of lymphocytes in patients with ileocolitis and universal colitis, the percentage of these cells was decreased because of an increase in both polymorphonuclear leukocytes and monocytes. In IBD and its subgroups, mean T lymphocytes, determined by the sheep red blood cell rosette technique, were not significantly different from the controls either in percentage or absolute number. Furthermore, no difference was noted between UC and CD. However, there seems to be a subpopulation of patients with UC or CD whose T cells are reduced below 1 SD of the mean. There was also no difference in the number of immunoglobulin-bearing B cells in both diseases; however, when the B cells were enumerated by their ability to rosette with antibody-complement-coated sheep cells (EAC), we found a marked decrease in percentage (P less than 0.001) and absolute number (P less than 0.0005) relative to the control population. The decrease bore a direct relation to the severity of the disease process and, although more marked in patients with UC, was present in CD also.", "contents": "The subpopulations of circulating white blood cells in inflammatory bowel disease. A detailed analysis of the species of lymphocytes was carried out in 58 patients with inflammatory bowel disease (IBD). These individuals were further divided into 31 with Crohn's disease (CD) and 27 with ulcerative colitis (UC). There were 13 CD patients with only small bowel involvement called \"regional enteritis\" and 18 who had some degree of colonic involvement called \"ileocolitis\". Similarly, the UC group was subdivided into 9 patients with disease confined to the rectosigmoid area called \"proctosigmoiditis\" and 18 with more extensive involvement called \"universal colitis\". We also studied 13 patients who had undergone previous colectomy and ileostomy and 78 healthy age- and sex-matched controls. Although there was no increase in the absolute number of lymphocytes in patients with ileocolitis and universal colitis, the percentage of these cells was decreased because of an increase in both polymorphonuclear leukocytes and monocytes. In IBD and its subgroups, mean T lymphocytes, determined by the sheep red blood cell rosette technique, were not significantly different from the controls either in percentage or absolute number. Furthermore, no difference was noted between UC and CD. However, there seems to be a subpopulation of patients with UC or CD whose T cells are reduced below 1 SD of the mean. There was also no difference in the number of immunoglobulin-bearing B cells in both diseases; however, when the B cells were enumerated by their ability to rosette with antibody-complement-coated sheep cells (EAC), we found a marked decrease in percentage (P less than 0.001) and absolute number (P less than 0.0005) relative to the control population. The decrease bore a direct relation to the severity of the disease process and, although more marked in patients with UC, was present in CD also."}
{"id": "PMID:1084852", "title": "Adhesion of human blood platelets to glass polymer surfaces. II. Demonstration of the presence of a natural platelet adhesion inhibitor in plasma and serum.", "content": "Adhesiveness of washed platelets resuspended in citrated plasma, serum, or several different media has been investigated. A method specific for quanititation of adhesion was used. Platelets suspended in saline of Tyrode's solution were found to be highly adhesive to glass, polyethylene, polyvinyl chloride, or Cuprophane. This adhesiveness of platelets to test surfaces decreased by nearly 50% when plasma was the suspension medium. When the suspension medium was serum, the decrease in adhesion was nearly 75%. Cohn fraction V also decreased the adhesiveness of platelets significantly, but highly purfied albumin had only a small effect. Several pharmacologic agents decreased platelet adhesiveness when added to platelets suspended in plasma or serum, but had negligible effect on the adhesiveness of platelets suspended in artificial media devoid of proteins. Normal washed platelets, when suspended in citrated plasma obtained from an afibrinogenemic donor or in normal serum, showed a significant decrease in adhesion compared to the same platelets suspended in normal citrated plasma. Addition of fibrinogen to afibrinogenemic plasma or normal serum restored the adhesiveness of platelets to normal levels. Normal platelets resuspended in plasma obtained from a thrombasthenic donor exhibited normal adhesiveness. These observations suggested that while fibrinogen promotes platelet adhesion, plasma or serum possess also an adhesion-inhibiting activity.", "contents": "Adhesion of human blood platelets to glass polymer surfaces. II. Demonstration of the presence of a natural platelet adhesion inhibitor in plasma and serum. Adhesiveness of washed platelets resuspended in citrated plasma, serum, or several different media has been investigated. A method specific for quanititation of adhesion was used. Platelets suspended in saline of Tyrode's solution were found to be highly adhesive to glass, polyethylene, polyvinyl chloride, or Cuprophane. This adhesiveness of platelets to test surfaces decreased by nearly 50% when plasma was the suspension medium. When the suspension medium was serum, the decrease in adhesion was nearly 75%. Cohn fraction V also decreased the adhesiveness of platelets significantly, but highly purfied albumin had only a small effect. Several pharmacologic agents decreased platelet adhesiveness when added to platelets suspended in plasma or serum, but had negligible effect on the adhesiveness of platelets suspended in artificial media devoid of proteins. Normal washed platelets, when suspended in citrated plasma obtained from an afibrinogenemic donor or in normal serum, showed a significant decrease in adhesion compared to the same platelets suspended in normal citrated plasma. Addition of fibrinogen to afibrinogenemic plasma or normal serum restored the adhesiveness of platelets to normal levels. Normal platelets resuspended in plasma obtained from a thrombasthenic donor exhibited normal adhesiveness. These observations suggested that while fibrinogen promotes platelet adhesion, plasma or serum possess also an adhesion-inhibiting activity."}
{"id": "PMID:1084854", "title": "[Sex difference in the circadian rhythm of corticotropin-releasing activity in the rat hypothalamus (author's transl)].", "content": "Sex difference in the circadian rhythm of the hypothalamic content of Corticotropin-Releasing Factor (CRF) and plasma corticosterone levels was examined in the rat. In the male rat the CRF content was higher in the afternoon than in the morning. In contrast, the hypothalamic CRF content in the female rat was higher in the morning. The peak value was found at 8 a.m. and it fell rapidly around noon with a concomitant sharp rise in the plasma corticosterone. Thus, the whole pattern of CRF content during a 24-hour period makes a mirror image of that of plasma corticosterone. The influence of gonadal hormones on the sex difference was then examined by following variations in the CRF content after ovariectomy. Even after chronic ovariectomy, essential features of the female pattern of CRF rhythm persisted: rapid fall of the CRF content was accompanied by a sharp rise in the plasma corticosterone level. It is noteworthy however that ovariectomy reduced the morning level of CRF activity without elevating the afternoon level, resulting in a peak shift toward noon. The female pattern of CRF rhythmpersisted similarly after bilateral ganglionectomy which is known to affect the biogenic amine rhythm in the pineal gland. The persistence of the female pattern may suggest some endogenous nature of the rhythm. It was then possibly that sex differentiation in the central nervous system (CNS) might be related with manifestation of the female pattern. In order to elucidate this point, postnatal development of circadian rhythm of CRF activity was examined in male and female rats separately. It was found that the CRF rhythm became manifest both in male and female rats around the third week of postnatal life and there was no essential difference in their rhythm pattern. In other words, both male and female rats begin their CRF rhythm with so-called male pattern, with higher values in the afternoon than in the end of the third week of life. In contrast, the CRF rhythm in females rats showed a marked change at ages of five to six weeks during which the onset of puberty intervened in our series of experiment;during this period, the CRF rhythm in female rats turned into the so-called female pattern, with higher values of CRF activity in the morning than in the afternoon. It was of interest that a marked rise in the plasma corticosterone, characteristic of mature female rats, concomitantly appeared. Further attempt were made to examine the effect of gonadal hormones in the periratal period. The hypothalamic CRF content in androgen sterilized female rats as well as in neonatally castrated male rats showed no circadian rhythm. The results implicate subtleness of hormonal effect in simulating physiological processes. In fact, CRF rhythms are variable depending on stages of the estrous cycle: during proestrus and estrus, the CRF content was markedly higher in the morning (9 a.m.) than in the afternoon (4 p.m.). But no significant difference was observed between them during diestrus I and II...", "contents": "[Sex difference in the circadian rhythm of corticotropin-releasing activity in the rat hypothalamus (author's transl)]. Sex difference in the circadian rhythm of the hypothalamic content of Corticotropin-Releasing Factor (CRF) and plasma corticosterone levels was examined in the rat. In the male rat the CRF content was higher in the afternoon than in the morning. In contrast, the hypothalamic CRF content in the female rat was higher in the morning. The peak value was found at 8 a.m. and it fell rapidly around noon with a concomitant sharp rise in the plasma corticosterone. Thus, the whole pattern of CRF content during a 24-hour period makes a mirror image of that of plasma corticosterone. The influence of gonadal hormones on the sex difference was then examined by following variations in the CRF content after ovariectomy. Even after chronic ovariectomy, essential features of the female pattern of CRF rhythm persisted: rapid fall of the CRF content was accompanied by a sharp rise in the plasma corticosterone level. It is noteworthy however that ovariectomy reduced the morning level of CRF activity without elevating the afternoon level, resulting in a peak shift toward noon. The female pattern of CRF rhythmpersisted similarly after bilateral ganglionectomy which is known to affect the biogenic amine rhythm in the pineal gland. The persistence of the female pattern may suggest some endogenous nature of the rhythm. It was then possibly that sex differentiation in the central nervous system (CNS) might be related with manifestation of the female pattern. In order to elucidate this point, postnatal development of circadian rhythm of CRF activity was examined in male and female rats separately. It was found that the CRF rhythm became manifest both in male and female rats around the third week of postnatal life and there was no essential difference in their rhythm pattern. In other words, both male and female rats begin their CRF rhythm with so-called male pattern, with higher values in the afternoon than in the end of the third week of life. In contrast, the CRF rhythm in females rats showed a marked change at ages of five to six weeks during which the onset of puberty intervened in our series of experiment;during this period, the CRF rhythm in female rats turned into the so-called female pattern, with higher values of CRF activity in the morning than in the afternoon. It was of interest that a marked rise in the plasma corticosterone, characteristic of mature female rats, concomitantly appeared. Further attempt were made to examine the effect of gonadal hormones in the periratal period. The hypothalamic CRF content in androgen sterilized female rats as well as in neonatally castrated male rats showed no circadian rhythm. The results implicate subtleness of hormonal effect in simulating physiological processes. In fact, CRF rhythms are variable depending on stages of the estrous cycle: during proestrus and estrus, the CRF content was markedly higher in the morning (9 a.m.) than in the afternoon (4 p.m.). But no significant difference was observed between them during diestrus I and II..."}
{"id": "PMID:1084858", "title": "In vitro effects of zinc chloride on spontaneous sheep red blood cell (e) rosette formation by lymphocytes from cancer patients and normal subjects.", "content": "The in vitro spontaneous formation of sheep red blood cell (SRBC) (E) rosettes by peripheral blood lymphocytes is altered in vitro by the addition of micro-molar quantities of zinc chloride. Appropriate concentrations of zinc chloride cause marked enhancement of rosette formation, both in normal subjects and in cancer patients with low initial E rosette values. The effect is dependent upon zinc treatment of the lymphocyte and not the SRBC. Other zinc-induced effects on E rosettes include retardation of temperature-dependent spontaneous decay, increased mechanical stability due to enhanced of \"capping.\" The formation of EAC rosettes is not altered by the presence of zinc.", "contents": "In vitro effects of zinc chloride on spontaneous sheep red blood cell (e) rosette formation by lymphocytes from cancer patients and normal subjects. The in vitro spontaneous formation of sheep red blood cell (SRBC) (E) rosettes by peripheral blood lymphocytes is altered in vitro by the addition of micro-molar quantities of zinc chloride. Appropriate concentrations of zinc chloride cause marked enhancement of rosette formation, both in normal subjects and in cancer patients with low initial E rosette values. The effect is dependent upon zinc treatment of the lymphocyte and not the SRBC. Other zinc-induced effects on E rosettes include retardation of temperature-dependent spontaneous decay, increased mechanical stability due to enhanced of \"capping.\" The formation of EAC rosettes is not altered by the presence of zinc."}
{"id": "PMID:1084862", "title": "Distribution of T and B lymphocytes in lymphoid tissue of infants and children.", "content": "Normal lymphoid tissue from children undergoing elective surgery was examined for T and B lymphocyte distribution. Although established for peripheral blood and bone marrow, T and B lymphocyte distributions have not been previously reported for lymph nodes, appendix, thymus, and spleen tissues in children. Thymus-dependent T cells were determined by the sheep erythrocyte rosette technique, and thymus-independent B cells were determined by the fluorescent labeling of surface immunoglobulins A (IgA), G (IgG), and M (IgM). Fifty percent of lymph node cells were either T or B cells; 65% of these cells were T lymphocytes, whereas 58% of B cells were of the IgM subclass. Less than half of the appendix cells were either T or B cells; 47% of these were T lymphocytes, and the remainder B lymphocytes had subclass distribution similar to that of lymph nodes but different from peripheral blood and bone marrow where B cells bearing IgG predominate. Thymus tissue contained 43% T cells and less than 1% B cells, but the spleen was composed largely of B cells, predominantly of the IgM type. Lymphoid tissue from nine children with either inflammatory or neoplastic diseases were studied and included for contrast. This paper establishes relative distribution values for T and B lymphocytes in normal lymphoid tissue and points out the potential use of this technique to quantitate deviations from normal in certain inflammatory and neoplastic diseases.", "contents": "Distribution of T and B lymphocytes in lymphoid tissue of infants and children. Normal lymphoid tissue from children undergoing elective surgery was examined for T and B lymphocyte distribution. Although established for peripheral blood and bone marrow, T and B lymphocyte distributions have not been previously reported for lymph nodes, appendix, thymus, and spleen tissues in children. Thymus-dependent T cells were determined by the sheep erythrocyte rosette technique, and thymus-independent B cells were determined by the fluorescent labeling of surface immunoglobulins A (IgA), G (IgG), and M (IgM). Fifty percent of lymph node cells were either T or B cells; 65% of these cells were T lymphocytes, whereas 58% of B cells were of the IgM subclass. Less than half of the appendix cells were either T or B cells; 47% of these were T lymphocytes, and the remainder B lymphocytes had subclass distribution similar to that of lymph nodes but different from peripheral blood and bone marrow where B cells bearing IgG predominate. Thymus tissue contained 43% T cells and less than 1% B cells, but the spleen was composed largely of B cells, predominantly of the IgM type. Lymphoid tissue from nine children with either inflammatory or neoplastic diseases were studied and included for contrast. This paper establishes relative distribution values for T and B lymphocytes in normal lymphoid tissue and points out the potential use of this technique to quantitate deviations from normal in certain inflammatory and neoplastic diseases."}
{"id": "PMID:1084863", "title": "Tube leukocyte adherence inhibition assay for the detection of anti-tumor immunity. I. Monocyte is the reactive cell.", "content": "Peripheral blood leukocytes (PBL) from breast cancer patients with early or localized cancer fail to adhere to glass in the presence of breast cancer extract. The same leukocytes do not react to unrelated tumour extracts. Enrichment and depletion of certain PBL populations from patients with apparently localized breast cancer indicated that the indicator and/or reactive cell manifesting non-adherence in the presence of appropriate tumour antigen was phagocytic, glass adherent in the absence of tumour antigen and had cell surface Fc-receptors. The cell involved, therefore, appears to be the circulating monocyte. These results show that in the tube LAI assay, the peripheral blood monocyte appears to react directly with the tumour antigen resulting in a loss of its property of adherence to glass.", "contents": "Tube leukocyte adherence inhibition assay for the detection of anti-tumor immunity. I. Monocyte is the reactive cell. Peripheral blood leukocytes (PBL) from breast cancer patients with early or localized cancer fail to adhere to glass in the presence of breast cancer extract. The same leukocytes do not react to unrelated tumour extracts. Enrichment and depletion of certain PBL populations from patients with apparently localized breast cancer indicated that the indicator and/or reactive cell manifesting non-adherence in the presence of appropriate tumour antigen was phagocytic, glass adherent in the absence of tumour antigen and had cell surface Fc-receptors. The cell involved, therefore, appears to be the circulating monocyte. These results show that in the tube LAI assay, the peripheral blood monocyte appears to react directly with the tumour antigen resulting in a loss of its property of adherence to glass."}
{"id": "PMID:1084864", "title": "Sulphydryl and disulphide groups in normal and irradiated epidermis.", "content": "The distribution of sulphydryl (SH) and disulphide (SS) groups in normal and irradiated epidermis of newly-born rats was studied by microspectrophotometric quantitation of the Mercury-Orange reaction. In order to obtain information on (1) the total content of both groups within the different strata of the epithelium, and (2) the mean SH and SS concentration, which is an indicator of the density of these groups per unit of tissue. The content of both groups increased in relation to the extent of irradiated area, whereas there was a decrease in the mean concentration. The increase in content of SH and SS would lead to augmented production of keratin substance previously reported in irradiated epithelia, coincidentally with an acanthotic response. The decrease in concentration of SH and SS indicates a destruction of these groups at cellular level, which may be due to membrane alterations. The findings would explain an acceleration of the keratinizing process together with modifications, both in quality and in quantity, in the horny substance.", "contents": "Sulphydryl and disulphide groups in normal and irradiated epidermis. The distribution of sulphydryl (SH) and disulphide (SS) groups in normal and irradiated epidermis of newly-born rats was studied by microspectrophotometric quantitation of the Mercury-Orange reaction. In order to obtain information on (1) the total content of both groups within the different strata of the epithelium, and (2) the mean SH and SS concentration, which is an indicator of the density of these groups per unit of tissue. The content of both groups increased in relation to the extent of irradiated area, whereas there was a decrease in the mean concentration. The increase in content of SH and SS would lead to augmented production of keratin substance previously reported in irradiated epithelia, coincidentally with an acanthotic response. The decrease in concentration of SH and SS indicates a destruction of these groups at cellular level, which may be due to membrane alterations. The findings would explain an acceleration of the keratinizing process together with modifications, both in quality and in quantity, in the horny substance."}
{"id": "PMID:1084865", "title": "Somatic aberration induction in Tradescantia occidentalis by neutrons, x- and gamma-radiations. I. Dosimetry.", "content": "The dosimetry is described for an investigation of the induction of somatic aberrations in Tradescantia occidentalis by substantially mono-energetic neutrons in the energy range 100 keV to 15 MeV, by 200 keV X-rays and cobalt-60 gamma-radiation. Spectrometry was carried out for both neutrons and X-rays. Neutron fluence was measured by uranium fission chambers. Two types of ionization chamber were employed for dose measurement. One chamber was manufactured of CH-plastic and filled with acetylene and the other of graphite and filled with carbon dioxide. Dosimetry for X- and gamma-radiation was by means of lithium fluoride thermoluminescent dosemeters calibrated against a Victoreen ionization chamber.", "contents": "Somatic aberration induction in Tradescantia occidentalis by neutrons, x- and gamma-radiations. I. Dosimetry. The dosimetry is described for an investigation of the induction of somatic aberrations in Tradescantia occidentalis by substantially mono-energetic neutrons in the energy range 100 keV to 15 MeV, by 200 keV X-rays and cobalt-60 gamma-radiation. Spectrometry was carried out for both neutrons and X-rays. Neutron fluence was measured by uranium fission chambers. Two types of ionization chamber were employed for dose measurement. One chamber was manufactured of CH-plastic and filled with acetylene and the other of graphite and filled with carbon dioxide. Dosimetry for X- and gamma-radiation was by means of lithium fluoride thermoluminescent dosemeters calibrated against a Victoreen ionization chamber."}
{"id": "PMID:1084866", "title": "Somatic aberration induction in Tradescantia occidentalis by neutrons, x- and gamma-radiations. II. Biological results, r.b.e. and o.e.r.", "content": "Biological results, including statistical features, are described for the irradiation of Tradescantia occidentalis with 250 kVp X-rays, cobalt-60 gamma-radiation and monoenergetic neutrons with energies between 0-08 and 15 MeV. The effect studied was that of the induction of pink sectors in the otherwise blue staminal hairs of the flowers at low doses of radiation. Statistical aspects of the results suggest that a fraction of the asynchronous cell population in the hairs is very sensitive to neutron radiation, but not necessarily to lower LET radiations. All the results were fitted by a least-squares method by polynomials of different degrees. Best fits to X- and gamma-ray data were provided by second-degree polynomials, and to the neutron data by either second- or third-degree polynomials. Limiting r.b.e. and o.e.r. values at low doses are derived. Some computed microdosimetric parameters are presented in comparison with the r.b.e. values. It is concluded that the effect studied is complex and may not provide a critical test of bio-physical theories of radiation effects.", "contents": "Somatic aberration induction in Tradescantia occidentalis by neutrons, x- and gamma-radiations. II. Biological results, r.b.e. and o.e.r. Biological results, including statistical features, are described for the irradiation of Tradescantia occidentalis with 250 kVp X-rays, cobalt-60 gamma-radiation and monoenergetic neutrons with energies between 0-08 and 15 MeV. The effect studied was that of the induction of pink sectors in the otherwise blue staminal hairs of the flowers at low doses of radiation. Statistical aspects of the results suggest that a fraction of the asynchronous cell population in the hairs is very sensitive to neutron radiation, but not necessarily to lower LET radiations. All the results were fitted by a least-squares method by polynomials of different degrees. Best fits to X- and gamma-ray data were provided by second-degree polynomials, and to the neutron data by either second- or third-degree polynomials. Limiting r.b.e. and o.e.r. values at low doses are derived. Some computed microdosimetric parameters are presented in comparison with the r.b.e. values. It is concluded that the effect studied is complex and may not provide a critical test of bio-physical theories of radiation effects."}
{"id": "PMID:1084867", "title": "Independence of the radiobiological oxygen constant, K, and the respiration rate of mammalian cells.", "content": "The radiosensitivity, and the radiobiological oxygen-constant K for mouse Ehrlich ascites cells depend on the pre-irradiation growth conditions. Measurements of the oxygen-consumption rate of cells grown by different methods have been made to ascertain whether the differences in radiobiological properties could be associated with different rates of respiration. Inhibition of respiration by sodium amytal had no significant effect on either the radiosensitivity or the estimate of K.", "contents": "Independence of the radiobiological oxygen constant, K, and the respiration rate of mammalian cells. The radiosensitivity, and the radiobiological oxygen-constant K for mouse Ehrlich ascites cells depend on the pre-irradiation growth conditions. Measurements of the oxygen-consumption rate of cells grown by different methods have been made to ascertain whether the differences in radiobiological properties could be associated with different rates of respiration. Inhibition of respiration by sodium amytal had no significant effect on either the radiosensitivity or the estimate of K."}
{"id": "PMID:1084868", "title": "The effects of x-irradiation on collagen metabolism in rat skin.", "content": "Collagen metabolism in rat skin and the response to X-radiation are studied during three consecutive hair-cycles. Collagen biosynthesis and turnover, assessed by the hydroxylation of [U-14C]-proline, proceeds as a function of the periodic hair-growth phenomenon. A single X-radiation treatment to the skin does not affect the amino-acid composition of collagen, but reduces the proline hydroxylation capacity of the fibroblasts. The initial damage to the tissue, resulting in a reduced collagen concentration of the skin, particularly noticeable after several hair-cycles, is at least partially responsible for the delayed skin lesions after irradiation.", "contents": "The effects of x-irradiation on collagen metabolism in rat skin. Collagen metabolism in rat skin and the response to X-radiation are studied during three consecutive hair-cycles. Collagen biosynthesis and turnover, assessed by the hydroxylation of [U-14C]-proline, proceeds as a function of the periodic hair-growth phenomenon. A single X-radiation treatment to the skin does not affect the amino-acid composition of collagen, but reduces the proline hydroxylation capacity of the fibroblasts. The initial damage to the tissue, resulting in a reduced collagen concentration of the skin, particularly noticeable after several hair-cycles, is at least partially responsible for the delayed skin lesions after irradiation."}
{"id": "PMID:1084869", "title": "The metabolites of catecholamines in urine of patients irradiated therapeutically.", "content": "The metabolites of catecholamines were determined in 24-hour urine samples of patients with genital carcinoma and treated by radio therapy. The patients were irradiated first with gamma-rays of radium and then with X-rays. The radium sources (80 mCi) were placed intracavitarily for 46 hours twice within 2 weeks. X-irradiation (800 R daily), applied 1 month after radium treatment, was delivered on four abdominal fields over 15 days. The quantities of excreted catecholamine metabolites during irradiation were compared with control values (obtained before irradiation) in the same patients. Gamma-irradiation provoked a significant increase in the excretion of 3-methoxy-4-hydroxy-mandelic acid, metadrenaline and normetadrenaline, as well as of homovanillic acid, whereas X-irradiation provoked only a significant increase in the excretion of free 3-methoxy-4-hydroxy-phenylglycol. The increased excretion might be explained: (1) in the case of radium application, by direct radiation-induced release of catecholamines from the peripheral symphathetic nerves; (2) in the case of X-irradiation, by putting in the motion the complex of early neuroendocrine reactions via irradiated adrenal medulla.", "contents": "The metabolites of catecholamines in urine of patients irradiated therapeutically. The metabolites of catecholamines were determined in 24-hour urine samples of patients with genital carcinoma and treated by radio therapy. The patients were irradiated first with gamma-rays of radium and then with X-rays. The radium sources (80 mCi) were placed intracavitarily for 46 hours twice within 2 weeks. X-irradiation (800 R daily), applied 1 month after radium treatment, was delivered on four abdominal fields over 15 days. The quantities of excreted catecholamine metabolites during irradiation were compared with control values (obtained before irradiation) in the same patients. Gamma-irradiation provoked a significant increase in the excretion of 3-methoxy-4-hydroxy-mandelic acid, metadrenaline and normetadrenaline, as well as of homovanillic acid, whereas X-irradiation provoked only a significant increase in the excretion of free 3-methoxy-4-hydroxy-phenylglycol. The increased excretion might be explained: (1) in the case of radium application, by direct radiation-induced release of catecholamines from the peripheral symphathetic nerves; (2) in the case of X-irradiation, by putting in the motion the complex of early neuroendocrine reactions via irradiated adrenal medulla."}
{"id": "PMID:1084872", "title": "The rates of electron transfer from ClUra- and ClUraH-to p-nitroacetophenone.", "content": "Pulse-radiolysis experiments demonstrate that both the radical ion CIUra-/- and the protonated radical CIUraH- Of chlorouracil will transfer electrons to p-nitroacetophenone (PNAP). The reaction of CIUraH- is complicated by unknown reaction with PNAP below pH 4-4. Rate constants measured for reactions (2) and (3) are kappa2=5-3 x 109 and kappa3=3-3 x 109 M-1 sec -1. See article. An independent determination of the rate-constant for dissociation of the radical ion (equation (1)) was also obtained (kappa1=0-7 x 105 sec -1). See article.", "contents": "The rates of electron transfer from ClUra- and ClUraH-to p-nitroacetophenone. Pulse-radiolysis experiments demonstrate that both the radical ion CIUra-/- and the protonated radical CIUraH- Of chlorouracil will transfer electrons to p-nitroacetophenone (PNAP). The reaction of CIUraH- is complicated by unknown reaction with PNAP below pH 4-4. Rate constants measured for reactions (2) and (3) are kappa2=5-3 x 109 and kappa3=3-3 x 109 M-1 sec -1. See article. An independent determination of the rate-constant for dissociation of the radical ion (equation (1)) was also obtained (kappa1=0-7 x 105 sec -1). See article."}
{"id": "PMID:1084873", "title": "Induction by gamma-radiation of DNA synthesis in radicle cells of germinating seeds of Pisum sativum l.", "content": "In radicle meristem cells of germinating seeds of the pea (Pisum sativum L) before the onset of replicative synthesis of DNA, irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR). Maximum isotope incorporation was noted during the first 2 hours after irradiation. Higher doses of radiation suppressed 3H-TdR incorporation. It was not seen after gamma-irradiation of air-dried seeds, nor after fast-neutron irradiation. The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent. It is suggested that the gamma-radiation-induced incorporation of 3H-TdR in meristem cells during the pre-replicative period may be connected with repair phenomena.", "contents": "Induction by gamma-radiation of DNA synthesis in radicle cells of germinating seeds of Pisum sativum l. In radicle meristem cells of germinating seeds of the pea (Pisum sativum L) before the onset of replicative synthesis of DNA, irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR). Maximum isotope incorporation was noted during the first 2 hours after irradiation. Higher doses of radiation suppressed 3H-TdR incorporation. It was not seen after gamma-irradiation of air-dried seeds, nor after fast-neutron irradiation. The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent. It is suggested that the gamma-radiation-induced incorporation of 3H-TdR in meristem cells during the pre-replicative period may be connected with repair phenomena."}
{"id": "PMID:1084874", "title": "Changes in morphogenesis and developmental enzyme levels in Dictyostelium discoideum after gamma irradiation.", "content": "Changes in the levels of specific activity of two enzymes believed to be involved in developmental regulation were observed after irradiating differentiating cells of Dictyostelium discoideum. Stimulation of the levels of specific activity of alkaline phosphatase occured after irradiation at the beginning of development and at the end of the aggregation period, but not after irradiation at the beginning of aggregation. A stimulation in UDP-glucose pyrophosphorylase specific activity was also observed, but to a lesser extent and only after irradiation at the end of aggregation. Dose-dependent delays in the appearance of peaks of specific activity were noted. The delay per unit dose was less when irradiation took place at the beginning of development as opposed to the beginning or end of the aggregation period. Radiation-induced delays in progression through visible developmental stages were almost identical to delays in enzyme appearance. Other radiation effects on morphogenesis included the induction of a migratory slug phase.", "contents": "Changes in morphogenesis and developmental enzyme levels in Dictyostelium discoideum after gamma irradiation. Changes in the levels of specific activity of two enzymes believed to be involved in developmental regulation were observed after irradiating differentiating cells of Dictyostelium discoideum. Stimulation of the levels of specific activity of alkaline phosphatase occured after irradiation at the beginning of development and at the end of the aggregation period, but not after irradiation at the beginning of aggregation. A stimulation in UDP-glucose pyrophosphorylase specific activity was also observed, but to a lesser extent and only after irradiation at the end of aggregation. Dose-dependent delays in the appearance of peaks of specific activity were noted. The delay per unit dose was less when irradiation took place at the beginning of development as opposed to the beginning or end of the aggregation period. Radiation-induced delays in progression through visible developmental stages were almost identical to delays in enzyme appearance. Other radiation effects on morphogenesis included the induction of a migratory slug phase."}
{"id": "PMID:1084879", "title": "Surface markers and other characteristics of the lymphocyte in chronic lymphocytic leukemia.", "content": "The vast majority of leukemic cells bear surface Ig. The class restriction of this surface Ig and other characteristics described for chronic lymphocytic leukemia (CLL) are considered as evidence for the monoclonality of the neoplastic proliferation. Different CLL cases with various expressions of surface Ig represent various degrees of block in the maturation of the lymphocytes. Through the use of other cell markers, it has been shown that most cases of CLL represent B cell neoplasia. Variations in the expression of the various B cell markers on the leukemic cells were observed. One case in which the leukemic cells were clearly T cell in character, and a single case of mixed B and T CLL, are described. The significance of the sheep red blood cell rosette-forming cells in CLL is discussed. Scanning electron microscopy (SEM) can be a useful adjunct to the identification of B- and T-derived lymphocytes. According to these criteria, leukemic cells are mostly of the B type, although variations in their surface architecture were noted. It is concluded that SEM alone cannot consistently distinguish between leukemic B and T cells. Electron microscope studies of mitogen-transformed CLL lymphocytes suggest that there is a residual normal B and T population of cells in addition to the predominant, abnormally reacting cells, which are mostly of B origin. Antigenic changes on the surface of CLL lymphocytes are suggestive of normal antigens present on young normal lymphocytes, rather than of the emergence of truly \"tumor-specific\" antigens. These antigens are independent of the T or B origin of the leukemia. CLL lymphocytes are shown to be defective in their ability to form caps with concanavalin A and anti-HL-A sera, and in their osmotic regulatory capacity.", "contents": "Surface markers and other characteristics of the lymphocyte in chronic lymphocytic leukemia. The vast majority of leukemic cells bear surface Ig. The class restriction of this surface Ig and other characteristics described for chronic lymphocytic leukemia (CLL) are considered as evidence for the monoclonality of the neoplastic proliferation. Different CLL cases with various expressions of surface Ig represent various degrees of block in the maturation of the lymphocytes. Through the use of other cell markers, it has been shown that most cases of CLL represent B cell neoplasia. Variations in the expression of the various B cell markers on the leukemic cells were observed. One case in which the leukemic cells were clearly T cell in character, and a single case of mixed B and T CLL, are described. The significance of the sheep red blood cell rosette-forming cells in CLL is discussed. Scanning electron microscopy (SEM) can be a useful adjunct to the identification of B- and T-derived lymphocytes. According to these criteria, leukemic cells are mostly of the B type, although variations in their surface architecture were noted. It is concluded that SEM alone cannot consistently distinguish between leukemic B and T cells. Electron microscope studies of mitogen-transformed CLL lymphocytes suggest that there is a residual normal B and T population of cells in addition to the predominant, abnormally reacting cells, which are mostly of B origin. Antigenic changes on the surface of CLL lymphocytes are suggestive of normal antigens present on young normal lymphocytes, rather than of the emergence of truly \"tumor-specific\" antigens. These antigens are independent of the T or B origin of the leukemia. CLL lymphocytes are shown to be defective in their ability to form caps with concanavalin A and anti-HL-A sera, and in their osmotic regulatory capacity."}
{"id": "PMID:1084885", "title": "New high-molecular decomposition products of natamycin (pimaricin) with intact lactone-ring.", "content": "Mild acid treatment of natamycin (IV) results in biologically inactive aponatamycin (VI), an amphoteric substance with some natamycin-like chemical and physical properties. Aponatamycin contains one natamycin- and one natamycinolide-moiety. More drastic acid degradation of natamycin eliminates the aminosugar under formation of the dimer (VII) of  the hypothetical aglycone of natamycin, natamycinolide (V) as well as a non-ionic compound, the dimer of the 12-decarboxy-11-anhydro analogue of natamycinolide.", "contents": "New high-molecular decomposition products of natamycin (pimaricin) with intact lactone-ring. Mild acid treatment of natamycin (IV) results in biologically inactive aponatamycin (VI), an amphoteric substance with some natamycin-like chemical and physical properties. Aponatamycin contains one natamycin- and one natamycinolide-moiety. More drastic acid degradation of natamycin eliminates the aminosugar under formation of the dimer (VII) of  the hypothetical aglycone of natamycin, natamycinolide (V) as well as a non-ionic compound, the dimer of the 12-decarboxy-11-anhydro analogue of natamycinolide."}
{"id": "PMID:1084880", "title": "A syngeneic metastatic tumor model in mice: the natural immune response of the host and its manipulation.", "content": "The cell-mediated immune response against a transplantable syngeneic metastatic solid tumor in mice was studied. The immune reactivity of spleen cells from tumor-bearing mice was found to vary during development of the tumor. For about a week after tumor transplantation, the spleen cells were able to protect recipient mice against challenge with tumor cells. Subsequently, the protective activity was replaced by an enhancing activity. Recipient mice that received tumor cells together with spleen cells from mice bearing tumors for about two or three weeks had a higher incidence of tumor takes and larger tumors than controls. This enhancement of tumor development was correlated with the size of the local tumor or metastases in the donors. The enhancing activity was found to be mediated by T lymphocytes and appeared to suppress the protective immune response of the recipients. We devised a system to strengthen the immune response of the host against the development of tumor metastases. In the tumor model used, removal of the local tumor after s.c. transplantation failed to prevent the development of lung metastases and death in most of the mice. However, syngeneic spleen cells which had been sensitized in vitro against tumor cells were found to serve as immunotherapeutic agents. Injection of such spleen cells into mice from which primary tumor implants had been removed surgically led to a markedly increased survival. Spleen cells from both normal and tumor-sensitized donors were effective, but splenocytes from mice bearing large tumors did not reduce metastatic development after sensitization in vitro. Thus, protection against the development of lethal metastases can be achieved with certain types of lymphocytes sensitized in vitro.", "contents": "A syngeneic metastatic tumor model in mice: the natural immune response of the host and its manipulation. The cell-mediated immune response against a transplantable syngeneic metastatic solid tumor in mice was studied. The immune reactivity of spleen cells from tumor-bearing mice was found to vary during development of the tumor. For about a week after tumor transplantation, the spleen cells were able to protect recipient mice against challenge with tumor cells. Subsequently, the protective activity was replaced by an enhancing activity. Recipient mice that received tumor cells together with spleen cells from mice bearing tumors for about two or three weeks had a higher incidence of tumor takes and larger tumors than controls. This enhancement of tumor development was correlated with the size of the local tumor or metastases in the donors. The enhancing activity was found to be mediated by T lymphocytes and appeared to suppress the protective immune response of the recipients. We devised a system to strengthen the immune response of the host against the development of tumor metastases. In the tumor model used, removal of the local tumor after s.c. transplantation failed to prevent the development of lung metastases and death in most of the mice. However, syngeneic spleen cells which had been sensitized in vitro against tumor cells were found to serve as immunotherapeutic agents. Injection of such spleen cells into mice from which primary tumor implants had been removed surgically led to a markedly increased survival. Spleen cells from both normal and tumor-sensitized donors were effective, but splenocytes from mice bearing large tumors did not reduce metastatic development after sensitization in vitro. Thus, protection against the development of lethal metastases can be achieved with certain types of lymphocytes sensitized in vitro."}
{"id": "PMID:1084881", "title": "Selection and enrichment of antigen-specific T lymphocyte populations.", "content": "Antigen-specific T lymphocytes bind in vitro to cells carrying alloantigens or to macrophages bearing antigen to which the lymphocytes were sensitized. This phenomenon is the basis of present procedures for the isolation and enrichment of antigen-specific T lymphocytes. The antigen-mediated adherence permits the removal of T cells with other specificities, which do not bind to the antigen-containing monolayer. The subsequent activation and multiplication of the antigen-bound cells further increases the proportion of the antigen-specific lymphocytes in culture. The selected cells, which are derived from a small fraction of the initial lymphocyte population, proliferate until they comprise up to 30% of the original total cell number. Another factor favoring enrichment is that unactivated cells do not survive well in culture whereas antigen-specific activated cells maintain their viability. Indeed, the selected cells can be maintained for a few weeks in culture. The activity of the selected lymphocytes is elevated toward the antigen used for enrichment, but lowered, or absent, toward other antigens. The progeny of the antigen-adherent lymphocytes are thus seen to be enriched for one antigen-specific subpopulation, and deprived of lymphocytes committed to other antigens. Cells obtained by this procedure should prove to be very useful for the functional analysis of T lymphocytes.", "contents": "Selection and enrichment of antigen-specific T lymphocyte populations. Antigen-specific T lymphocytes bind in vitro to cells carrying alloantigens or to macrophages bearing antigen to which the lymphocytes were sensitized. This phenomenon is the basis of present procedures for the isolation and enrichment of antigen-specific T lymphocytes. The antigen-mediated adherence permits the removal of T cells with other specificities, which do not bind to the antigen-containing monolayer. The subsequent activation and multiplication of the antigen-bound cells further increases the proportion of the antigen-specific lymphocytes in culture. The selected cells, which are derived from a small fraction of the initial lymphocyte population, proliferate until they comprise up to 30% of the original total cell number. Another factor favoring enrichment is that unactivated cells do not survive well in culture whereas antigen-specific activated cells maintain their viability. Indeed, the selected cells can be maintained for a few weeks in culture. The activity of the selected lymphocytes is elevated toward the antigen used for enrichment, but lowered, or absent, toward other antigens. The progeny of the antigen-adherent lymphocytes are thus seen to be enriched for one antigen-specific subpopulation, and deprived of lymphocytes committed to other antigens. Cells obtained by this procedure should prove to be very useful for the functional analysis of T lymphocytes."}
{"id": "PMID:1084886", "title": "Comparison of the chemical, physical, and survival properties of normal and Z-variant alpha-1-antitrypsins.", "content": "A procedure has been developed for the purification of Z-type alpha-1-antitrypsin (alpha-1-AT) which is rapid, gentle, and results in good yields. From 4 units (750 ml) of fresh human plasma, obtained from two individuals possessing the Pizz phenotype, 53 mg of pure Z-type alpha-1-AT was obtained. The preparation was homogeneous by the criteria of polyacrylamide gel electrophoresis, both in the presence and absence of sodium dodecyl sulfate, and by analytical ultracentrifugation. When compared to pure alpha-1-AT from plasma of individuals possessing the normal PiMM phenotype, the two proteins were indistinguishable with respect to amino acid composition, sedimentation coefficient (s20w of 3.33 for both M and Z), molecular weight (51,000 by sodium dodecyl sulfate gel electrophoresis and 47,000 by sedimentation equilibrium for both M and Z), and trypsin-combining ratio (0.91 for Z and 0.99 for M). The only difference which was observed between the variant forms of alpha-1-AT was in the carbohydrate composition. The Z-type alpha1-AT contains between 20 and 25% less carbohydrate than the M-type alpha-1-AT. Specifically, the Z-type alpha-1-AT is deficient in 1 glucosamine residue, 3 neutral sugar residues (1 mannose and 2 galactose), and 2 sialic acid residues. Although the Z-variant is deficient in sialic acid, its survival time in the serum of a rabbit was not significantly different from that of M-type alpha-1-AT.", "contents": "Comparison of the chemical, physical, and survival properties of normal and Z-variant alpha-1-antitrypsins. A procedure has been developed for the purification of Z-type alpha-1-antitrypsin (alpha-1-AT) which is rapid, gentle, and results in good yields. From 4 units (750 ml) of fresh human plasma, obtained from two individuals possessing the Pizz phenotype, 53 mg of pure Z-type alpha-1-AT was obtained. The preparation was homogeneous by the criteria of polyacrylamide gel electrophoresis, both in the presence and absence of sodium dodecyl sulfate, and by analytical ultracentrifugation. When compared to pure alpha-1-AT from plasma of individuals possessing the normal PiMM phenotype, the two proteins were indistinguishable with respect to amino acid composition, sedimentation coefficient (s20w of 3.33 for both M and Z), molecular weight (51,000 by sodium dodecyl sulfate gel electrophoresis and 47,000 by sedimentation equilibrium for both M and Z), and trypsin-combining ratio (0.91 for Z and 0.99 for M). The only difference which was observed between the variant forms of alpha-1-AT was in the carbohydrate composition. The Z-type alpha1-AT contains between 20 and 25% less carbohydrate than the M-type alpha-1-AT. Specifically, the Z-type alpha-1-AT is deficient in 1 glucosamine residue, 3 neutral sugar residues (1 mannose and 2 galactose), and 2 sialic acid residues. Although the Z-variant is deficient in sialic acid, its survival time in the serum of a rabbit was not significantly different from that of M-type alpha-1-AT."}
{"id": "PMID:1084882", "title": "Surface phenotype of nonadherent peritoneal cells effecting cell-mediated cytotoxicity in vitro for allogeneic and syngeneic murine sarcoma cells.", "content": "The participation of B cells in cell-mediated cytotoxicity (CMC) for allogeneic and syngeneic murine sarcoma cells induced with 3-methylcholanthrene was investigated. Primed nonadherent peritoneal cells (NAPC) were treated with antisera against lymphocyte surface antigens and complement, and residual CMC activity was measured with the [3H]proline microassay. The antisera (anti-PC.1 and various anti-Ig sera) used for this purpose were highly reactive with B cells according to established serologic criteria. Elimination of cells that carried PC.1 or Ig on their surface did not diminish CMC of NAPC for allogeneic or syngeneic tumor cells. Exposure of cytotoxic NAPC to various anti-Ig antisera (without complement) before and during the CMC assay did not inhibit CMC either. We conclude that under these conditions CMC is not mediated by B cells, nor is it dependent on their presence. In addition, our findings do not implicate any of the usual classes and types of Ig covered by the Ig antisera used in this study as constituting specific receptors on the effector T cells of CMC.", "contents": "Surface phenotype of nonadherent peritoneal cells effecting cell-mediated cytotoxicity in vitro for allogeneic and syngeneic murine sarcoma cells. The participation of B cells in cell-mediated cytotoxicity (CMC) for allogeneic and syngeneic murine sarcoma cells induced with 3-methylcholanthrene was investigated. Primed nonadherent peritoneal cells (NAPC) were treated with antisera against lymphocyte surface antigens and complement, and residual CMC activity was measured with the [3H]proline microassay. The antisera (anti-PC.1 and various anti-Ig sera) used for this purpose were highly reactive with B cells according to established serologic criteria. Elimination of cells that carried PC.1 or Ig on their surface did not diminish CMC of NAPC for allogeneic or syngeneic tumor cells. Exposure of cytotoxic NAPC to various anti-Ig antisera (without complement) before and during the CMC assay did not inhibit CMC either. We conclude that under these conditions CMC is not mediated by B cells, nor is it dependent on their presence. In addition, our findings do not implicate any of the usual classes and types of Ig covered by the Ig antisera used in this study as constituting specific receptors on the effector T cells of CMC."}
{"id": "PMID:1084883", "title": "Induction of cell-mediated immunity in the mouse: circumstantial evidence for highly immunogenic antigen in the regional lymph nodes following skin painting with contact sensitizing agents.", "content": "This paper describes an investigation of why contact sensitizing agents cause strong cell-mediated immunity. Contact sensitivity was induced in mice by painting the skin with 4-ethoxymethylene-2-phenyloxazolone (oxazolone), and measured by the increase of ear thickness following challenge six days later. Reactivity was transferred by taking the regional lymph node cells from mice 18 h after immunization and injecting them into the footpads of recipients. This \"18-h transfer\" has several characteristics. As few as 2 X 10(4) cells were effective. The donor lymph node cells were best taken one to three days after immunization, were less effective on day 4 and virtually inactive by day 7. The recipients developed contact sensitivity when challenged on day 4, but lacked sensitivity when challenged on days 1 and 2 after transfer. The transferred cells were still active after treatment with anti-theta serum and complement. They also resisted 2,000 R in vitro, mitomycin, vinblastine, and inhibitors of protein synthesis such as emetine, cycloheximide and puromycin. The transfer was prevented by treatment with trypsin, freeze-thawing, and heating at 56 C. Plasma membranes were also immunogenic. The evidence suggests that the \"18-h transfer\" is a special type of active immunization, not due to ordinary free oxazolone, and that the agent is present within the lymph node in a free oxazolone, and that the agent is present within the lymph node in a specially immunogenic location or form.", "contents": "Induction of cell-mediated immunity in the mouse: circumstantial evidence for highly immunogenic antigen in the regional lymph nodes following skin painting with contact sensitizing agents. This paper describes an investigation of why contact sensitizing agents cause strong cell-mediated immunity. Contact sensitivity was induced in mice by painting the skin with 4-ethoxymethylene-2-phenyloxazolone (oxazolone), and measured by the increase of ear thickness following challenge six days later. Reactivity was transferred by taking the regional lymph node cells from mice 18 h after immunization and injecting them into the footpads of recipients. This \"18-h transfer\" has several characteristics. As few as 2 X 10(4) cells were effective. The donor lymph node cells were best taken one to three days after immunization, were less effective on day 4 and virtually inactive by day 7. The recipients developed contact sensitivity when challenged on day 4, but lacked sensitivity when challenged on days 1 and 2 after transfer. The transferred cells were still active after treatment with anti-theta serum and complement. They also resisted 2,000 R in vitro, mitomycin, vinblastine, and inhibitors of protein synthesis such as emetine, cycloheximide and puromycin. The transfer was prevented by treatment with trypsin, freeze-thawing, and heating at 56 C. Plasma membranes were also immunogenic. The evidence suggests that the \"18-h transfer\" is a special type of active immunization, not due to ordinary free oxazolone, and that the agent is present within the lymph node in a free oxazolone, and that the agent is present within the lymph node in a specially immunogenic location or form."}
{"id": "PMID:1084888", "title": "Axonal transport of [3H]serotonin in an identified neuron of Aplysia californica.", "content": "A population of characteristic ellipsoidal dense-core vesicles was identified in axons of the giant cerebral neuron of the mollusc Aplysia. We injected [3H]serotonin into the cell body of this identified serotonergic neuron in the isolated central nervous system in order to study the subcellular components associated with the neurotransmitter. Subcellular fractionation by differential centrifugation indicated that injected serotonin was rapidly taken up into particulate form. [3H]Serotonin appeared in the axons within 2 h after injection, and export continued at a constant rate of 6% of the total in the neuron/h thereafter. The dependence of the total amounts of [3H]serotonin which appeared in the axons in 6 h (export from the cell body) on the amounts injected was consistent with the idea that export is a saturable process, possibly depending on the capacity of somatic vesicles or of some unidentified carrier for serotonin. [3H]Serotonin moved into both major branches of the axon, where it was translocated rapidly. The transmitter, which was shown by autoradiography to be restricted to the axons of the injected cell, was distributed along axons in accumulations of radioactivity; in contrast, its precursor, [5-3H]hydroxytryptophan, moved slowly along axons in a smooth, declining curve, its kinetics consistent with diffusion. Quantitative electron microscope autoradiography revealed that the dense-core vesicles and the cytosol of axons fixed with glutaraldehyde were labeled with [3H]serotonin.", "contents": "Axonal transport of [3H]serotonin in an identified neuron of Aplysia californica. A population of characteristic ellipsoidal dense-core vesicles was identified in axons of the giant cerebral neuron of the mollusc Aplysia. We injected [3H]serotonin into the cell body of this identified serotonergic neuron in the isolated central nervous system in order to study the subcellular components associated with the neurotransmitter. Subcellular fractionation by differential centrifugation indicated that injected serotonin was rapidly taken up into particulate form. [3H]Serotonin appeared in the axons within 2 h after injection, and export continued at a constant rate of 6% of the total in the neuron/h thereafter. The dependence of the total amounts of [3H]serotonin which appeared in the axons in 6 h (export from the cell body) on the amounts injected was consistent with the idea that export is a saturable process, possibly depending on the capacity of somatic vesicles or of some unidentified carrier for serotonin. [3H]Serotonin moved into both major branches of the axon, where it was translocated rapidly. The transmitter, which was shown by autoradiography to be restricted to the axons of the injected cell, was distributed along axons in accumulations of radioactivity; in contrast, its precursor, [5-3H]hydroxytryptophan, moved slowly along axons in a smooth, declining curve, its kinetics consistent with diffusion. Quantitative electron microscope autoradiography revealed that the dense-core vesicles and the cytosol of axons fixed with glutaraldehyde were labeled with [3H]serotonin."}
{"id": "PMID:1084889", "title": "Human serum binding capacity and affinity for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol.", "content": "25-Hydroxyvitamin D-binding capacity and affinity were studied in human cord, adult, and maternal sera, and in sera from women receiving oral contraceptives, by in vitro satuaration analyses employing dextran-coated charcoal to adsorb unbound sterol. 25-Hydroxyergocalciferol and 25-hydroxycholecalciferol were equipotent in their ability to displace 3H 25-hydroxycholecalciferol from human serum binding sites. At 0C, the apparent dissociation constant for the serum binding of 25-hydroxycholecalciferol was low (Kd=8x 10-10M). Cord and adult sera had a similar 25-hydroxycholecalciferol binding capacity (1.8 x 10-6M), but the binding capacity of maternal sera and the sera from women receiving oral contraceptives was significantly higher. At physiological serum concentrations of 25-hydroxyvitamin D (5 x 10-8M), only 2-3% of human serum 25-hydroxyvitamin D-bindig sites are occupied.", "contents": "Human serum binding capacity and affinity for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol. 25-Hydroxyvitamin D-binding capacity and affinity were studied in human cord, adult, and maternal sera, and in sera from women receiving oral contraceptives, by in vitro satuaration analyses employing dextran-coated charcoal to adsorb unbound sterol. 25-Hydroxyergocalciferol and 25-hydroxycholecalciferol were equipotent in their ability to displace 3H 25-hydroxycholecalciferol from human serum binding sites. At 0C, the apparent dissociation constant for the serum binding of 25-hydroxycholecalciferol was low (Kd=8x 10-10M). Cord and adult sera had a similar 25-hydroxycholecalciferol binding capacity (1.8 x 10-6M), but the binding capacity of maternal sera and the sera from women receiving oral contraceptives was significantly higher. At physiological serum concentrations of 25-hydroxyvitamin D (5 x 10-8M), only 2-3% of human serum 25-hydroxyvitamin D-bindig sites are occupied."}
{"id": "PMID:1084890", "title": "Studies on human antihemophilic factor. Evidence for a covalently linked subunit structure.", "content": "When purified antihemophilic factor (Factor VIII) was rechromatographed on 4% agarose in 0.15 M NaCl or 1.0 M NaCl, a single protein peak, containing both procoagulant activity and von Willebrand factor activity, as defined by ristocetin-induced platelet aggregation, was eluted in the void volume. Purified Factor VIII immediately lost about 30% of its procoagulant activity when dissolved in 0.25 M CaCl2, and when rechromatographed on 4% agarose in 0.25 M CaCl2, the protein peak and von Willebrand factor activity remained coincident in the void volume; however, most of the remaining procoagulant activity was eluted after the void volume. The elution position of Factor VIII procoagulant activity from 4% agarose in 0.25 M CaCl2, and hence its apparent molecular weight, varied with the protein concentration applied to the column; at low protein concentrations it was eluted close to the inner volume. Yet on Sephadex G-200 in 0.25 M CaCl2, the protein and procoagulant activity were eluted together in the void volume. These observations suggested that the Factor VIII procoagulant activity was not eluting according to size or shape, but was adsorbing to some extent to the agarose. Isolated activity peak material from the 0.25 M CaCl2 columns contained protein and had a typical ultraviolet spectrum. Even at high concentrations, the protein contained no thrombin, Factors IX, X, or Xa activity, or detectable phospholipid. In addition to Factor VIII procoagulant activity, which could be inactivated by a human antibody to Factor VIII, the activity peak protein also contained von Willebrand factor activity. Like native Factor VIII and the void volume protein, the activity peak contained protein that did not enter a sodium dodecyl sulfate 5% polyacrylamide gel in the absence of reducing reagent. After reduction of disulfide bonds, several subunits ranging from 195,000 to 30,000 daltons were observed. These results indicate that the protein in the shifted Factor VIII procoagulant activity peak is large and that its anomalous elution pattern from 4% agarose in 0.25 M CaCl2 results from interaction with the agarose. The Factor VIII-like properties of the activity peak protein and its electrophoretic pattern on sodium dodecyl sulfate gels suggest that it is a species of Factor VIII modified by proteolytic cleavage. These results allow an interpretation that is different from the recently proposed \"carrier protein-small active subunit\" hypotheses for the structure-function relationships of the Factor VIII molecule.", "contents": "Studies on human antihemophilic factor. Evidence for a covalently linked subunit structure. When purified antihemophilic factor (Factor VIII) was rechromatographed on 4% agarose in 0.15 M NaCl or 1.0 M NaCl, a single protein peak, containing both procoagulant activity and von Willebrand factor activity, as defined by ristocetin-induced platelet aggregation, was eluted in the void volume. Purified Factor VIII immediately lost about 30% of its procoagulant activity when dissolved in 0.25 M CaCl2, and when rechromatographed on 4% agarose in 0.25 M CaCl2, the protein peak and von Willebrand factor activity remained coincident in the void volume; however, most of the remaining procoagulant activity was eluted after the void volume. The elution position of Factor VIII procoagulant activity from 4% agarose in 0.25 M CaCl2, and hence its apparent molecular weight, varied with the protein concentration applied to the column; at low protein concentrations it was eluted close to the inner volume. Yet on Sephadex G-200 in 0.25 M CaCl2, the protein and procoagulant activity were eluted together in the void volume. These observations suggested that the Factor VIII procoagulant activity was not eluting according to size or shape, but was adsorbing to some extent to the agarose. Isolated activity peak material from the 0.25 M CaCl2 columns contained protein and had a typical ultraviolet spectrum. Even at high concentrations, the protein contained no thrombin, Factors IX, X, or Xa activity, or detectable phospholipid. In addition to Factor VIII procoagulant activity, which could be inactivated by a human antibody to Factor VIII, the activity peak protein also contained von Willebrand factor activity. Like native Factor VIII and the void volume protein, the activity peak contained protein that did not enter a sodium dodecyl sulfate 5% polyacrylamide gel in the absence of reducing reagent. After reduction of disulfide bonds, several subunits ranging from 195,000 to 30,000 daltons were observed. These results indicate that the protein in the shifted Factor VIII procoagulant activity peak is large and that its anomalous elution pattern from 4% agarose in 0.25 M CaCl2 results from interaction with the agarose. The Factor VIII-like properties of the activity peak protein and its electrophoretic pattern on sodium dodecyl sulfate gels suggest that it is a species of Factor VIII modified by proteolytic cleavage. These results allow an interpretation that is different from the recently proposed \"carrier protein-small active subunit\" hypotheses for the structure-function relationships of the Factor VIII molecule."}
{"id": "PMID:1084893", "title": "Obstructive airway disease associated with heterozygous alpha-1-antitrypsin deficiency.", "content": "Twenty-eight members of one family were investigated for the presence of pulmonary disease concurrent with variable (ZZ, MZ, or MS) degrees of alpha-1 antitrypsin deficiency. Three symptomatic siblings (2, ZZ; 1, MZ) had abnormal pulmonary scans and abnormal routine pulmonary function studies. Five asymptomatic heterozygotes, three of whom had never smoked and were the youngest studied, had distinctly abnormal closing volumes. Three of the five patients also had abnormal perfusion dynamics when scanning was done in the upright position. Pulmonary dysfunction occurs in asymptomatic heterozygotes but requires the use of sensitive techniques for its appreciation.", "contents": "Obstructive airway disease associated with heterozygous alpha-1-antitrypsin deficiency. Twenty-eight members of one family were investigated for the presence of pulmonary disease concurrent with variable (ZZ, MZ, or MS) degrees of alpha-1 antitrypsin deficiency. Three symptomatic siblings (2, ZZ; 1, MZ) had abnormal pulmonary scans and abnormal routine pulmonary function studies. Five asymptomatic heterozygotes, three of whom had never smoked and were the youngest studied, had distinctly abnormal closing volumes. Three of the five patients also had abnormal perfusion dynamics when scanning was done in the upright position. Pulmonary dysfunction occurs in asymptomatic heterozygotes but requires the use of sensitive techniques for its appreciation."}
{"id": "PMID:1084894", "title": "Low molecular weight immunoglobulins in Rana catesbeiana tadpoles.", "content": "Rana catesbeiana tadpoles formed high and low m.w. antibodies in response to immunization with a bacteriophage. Although the neutralizing activity associated with the low m.w. immunoglobulins was relatively weak, the existence of antibodies in this class was clearly demonstrated by radioimmunoelectrophoresis. Moreover, two antigenically distinct variants of the low m.w. antibodies were detected. These were serologically indistinguishable from the two types of low m.w. immunoglobulin previously isolated from the serum of adult frogs of this species.", "contents": "Low molecular weight immunoglobulins in Rana catesbeiana tadpoles. Rana catesbeiana tadpoles formed high and low m.w. antibodies in response to immunization with a bacteriophage. Although the neutralizing activity associated with the low m.w. immunoglobulins was relatively weak, the existence of antibodies in this class was clearly demonstrated by radioimmunoelectrophoresis. Moreover, two antigenically distinct variants of the low m.w. antibodies were detected. These were serologically indistinguishable from the two types of low m.w. immunoglobulin previously isolated from the serum of adult frogs of this species."}
{"id": "PMID:1084895", "title": "Function of 2-mercaptoethanol as a macrophage substitute in the primary immune response in vitro.", "content": "The mechanism by which 2-ME acts as a macrophage-substitute for the induction of a primary PFC response to SRC in vitro was studied in macrophage-depleted mouse spleen cell cultures. 2-ME could replace macrophages only in FCS-supplemented cultures. Evidence is presented that the function of 2-ME is independent of residual macrophages. Neither normal nor macrophage-depleted spleen cell cultures from congenitally athymic nude mice supplemented with 2-ME, with or without FCS, could give rise to a primary in vitro anti-SRC immune response. 2-ME, at an optimal concentration of 10(-5) M, induced DNA synthesis in normal and macrophage-depleted spleen cells in both FCS-containing and serum-free cultures. The peak response occurred on day 3. The stimulation was accompanied by a polyclonal B cell activation to antibody secretion which was much more pronounced in FCS-containing than in serum-free cultures. Spleen cells from nude mice showed a weaker DNA stimulation than did cells from normal mice in FCS-containing cultures, and nearly no response under serum-free conditions. T cells obtained by a nylon column adherence method from normal mouse spleen cells showed good DNA synthetic responses in FCS-containing, but no response in serum-free cultures. These results show that 2-ME has weak mitogenic activity for B cells, and in combination with FCS, strong mitogenic activity for T cells. Since the macrophage provides stimulation to the T cell in the primary anti-SRC PFC response in vitro, these results suggest that the direct mitogenic activity of 2-ME with FCS on T cells provides the functional substitution for macrophages.", "contents": "Function of 2-mercaptoethanol as a macrophage substitute in the primary immune response in vitro. The mechanism by which 2-ME acts as a macrophage-substitute for the induction of a primary PFC response to SRC in vitro was studied in macrophage-depleted mouse spleen cell cultures. 2-ME could replace macrophages only in FCS-supplemented cultures. Evidence is presented that the function of 2-ME is independent of residual macrophages. Neither normal nor macrophage-depleted spleen cell cultures from congenitally athymic nude mice supplemented with 2-ME, with or without FCS, could give rise to a primary in vitro anti-SRC immune response. 2-ME, at an optimal concentration of 10(-5) M, induced DNA synthesis in normal and macrophage-depleted spleen cells in both FCS-containing and serum-free cultures. The peak response occurred on day 3. The stimulation was accompanied by a polyclonal B cell activation to antibody secretion which was much more pronounced in FCS-containing than in serum-free cultures. Spleen cells from nude mice showed a weaker DNA stimulation than did cells from normal mice in FCS-containing cultures, and nearly no response under serum-free conditions. T cells obtained by a nylon column adherence method from normal mouse spleen cells showed good DNA synthetic responses in FCS-containing, but no response in serum-free cultures. These results show that 2-ME has weak mitogenic activity for B cells, and in combination with FCS, strong mitogenic activity for T cells. Since the macrophage provides stimulation to the T cell in the primary anti-SRC PFC response in vitro, these results suggest that the direct mitogenic activity of 2-ME with FCS on T cells provides the functional substitution for macrophages."}
{"id": "PMID:1084896", "title": "Immunogen structure and cell selection. I. Cellular requirements for the in vitro response to moderately haptenated forms of the solid-phase immunogen DNP-O-Bio-Gel-P.", "content": "The cellular requirements for the in vitro response to DNP-O-Bio-Gel-P were determined. Results of these experiments indicate that: 1) splenic adherent cells are required for generation of anti-hapten PFC to the solid phase immunogen; and 2) cultured nude-mouse spleen cells have far less capacity to respond to the immunogen than do cells from wild type mice. These experiments suggest that both T and B cells are required for responsiveness to the moderately haptenated form of DNP-O-Bio-Gel-P.", "contents": "Immunogen structure and cell selection. I. Cellular requirements for the in vitro response to moderately haptenated forms of the solid-phase immunogen DNP-O-Bio-Gel-P. The cellular requirements for the in vitro response to DNP-O-Bio-Gel-P were determined. Results of these experiments indicate that: 1) splenic adherent cells are required for generation of anti-hapten PFC to the solid phase immunogen; and 2) cultured nude-mouse spleen cells have far less capacity to respond to the immunogen than do cells from wild type mice. These experiments suggest that both T and B cells are required for responsiveness to the moderately haptenated form of DNP-O-Bio-Gel-P."}
{"id": "PMID:1084897", "title": "Two functionally distinct anti-tumor effector cells isolated from primary murine sarcoma virus-induced tumors.", "content": "The ability of cells from primary MSV-induced tumors to function as effector cells in vitro was evaluated. Host cells were isolated by enzymatic disaggregation of the tumor and fractionated by sedimentation velocity at unit gravity on a Ficoll gradient. Characterization of these cells indicated that 30 to 40 % were T lymphocytes, about 50% were macrophages and less than 5% were B lymphocytes. Two different functional activities were mediated by these cells: cytolysis, as measured by the CRA, and inhibition of proliferation, as measured by the GIA. The effector cells in the CRA were T cells with sedimentation velocities of 3.5 to 4.0 mm/hr, whereas those cells which mediated the GIA were presumably macrophages and displayed a heterogeneity in size two peak sedimentation velocities, one at 4.0 mm/hr and another at 6.0 mm/hr. Activity by the effector cells in the CRA was antigen specific in contrast to the activity in the GIA which was directed against cells which did not carry detectable cross-reacting antigens.", "contents": "Two functionally distinct anti-tumor effector cells isolated from primary murine sarcoma virus-induced tumors. The ability of cells from primary MSV-induced tumors to function as effector cells in vitro was evaluated. Host cells were isolated by enzymatic disaggregation of the tumor and fractionated by sedimentation velocity at unit gravity on a Ficoll gradient. Characterization of these cells indicated that 30 to 40 % were T lymphocytes, about 50% were macrophages and less than 5% were B lymphocytes. Two different functional activities were mediated by these cells: cytolysis, as measured by the CRA, and inhibition of proliferation, as measured by the GIA. The effector cells in the CRA were T cells with sedimentation velocities of 3.5 to 4.0 mm/hr, whereas those cells which mediated the GIA were presumably macrophages and displayed a heterogeneity in size two peak sedimentation velocities, one at 4.0 mm/hr and another at 6.0 mm/hr. Activity by the effector cells in the CRA was antigen specific in contrast to the activity in the GIA which was directed against cells which did not carry detectable cross-reacting antigens."}
{"id": "PMID:1084898", "title": "B lymphocytes in the guinea pig thymus are specifically localized in the central area.", "content": "Since the central area is an integral part of the guinea pig thymus, the cells in this area were compared with those in the thymic cortex and medulla in cryostat-sections by using methods for demonstration of E-, EA-, EAC-adherence and surface membrane immunoglobulins. In the extra cortical central area (ECCA) 15 to 25% of the lymphocytes showed EAC-adherence and 5 to 10% appeared to bear surface membrane immunoglobulins (SIg). In the lymph sinuses up to 70% of the lymphocytes were EAC- and SIg-positive. A small amount of EAC-adhering cells was present in the medulla of the central area. Cortical lymphocytes were EAC- and SIg-negative. From these results we conclude that in the guinea pig thymus B lymphocytes are specifically localized in the central area.", "contents": "B lymphocytes in the guinea pig thymus are specifically localized in the central area. Since the central area is an integral part of the guinea pig thymus, the cells in this area were compared with those in the thymic cortex and medulla in cryostat-sections by using methods for demonstration of E-, EA-, EAC-adherence and surface membrane immunoglobulins. In the extra cortical central area (ECCA) 15 to 25% of the lymphocytes showed EAC-adherence and 5 to 10% appeared to bear surface membrane immunoglobulins (SIg). In the lymph sinuses up to 70% of the lymphocytes were EAC- and SIg-positive. A small amount of EAC-adhering cells was present in the medulla of the central area. Cortical lymphocytes were EAC- and SIg-negative. From these results we conclude that in the guinea pig thymus B lymphocytes are specifically localized in the central area."}
{"id": "PMID:1084899", "title": "Requirements for induction of T cell tolerance to DNFB: efficiency of membrane-associated DNFB.", "content": "Tolerance to contact sensitization with DNFB, a T cell-dependent phenomenon, was induced in mice by preparations of DNFB coupled to mouse RBC or spleen cells. Such tolerance is dose related, wanes with time, and can be transferred to normal animals with lymphoid cells (presumably containing suppressors). Tolerance to DNFB-RBC can be produced by whole DNFB-RBC, by ghosts of these cells, by sonicates of the ghosts, and by detergent-treated DNFB-RBC ghosts. Tolerance cannot be produced by larger amounts of DNFB-RBC components not associated with membrane. The ability of various DNP compounds to stimulate DNA synthesis in DNFB-sensitized cells also correlates with their ability to bind to protein components; i.e., DNFB is a far more efficient stimulator than DNBSO, whereas DNPlysine does not stimulate at all. Thus, the ability to sensitize or to tolerize with DNFB congeners is related to their ability to couple to proteins. It appears that the active induction of T cell tolerance requires that tolerogen be coupled to cell membranes. Since both T cell sensitization and tolerance to DNFB are best produced by DNFB-membrane, the actual occurrence of one state or the other must depend on the molecular method of \"presentation\" of DNFB-membrane.", "contents": "Requirements for induction of T cell tolerance to DNFB: efficiency of membrane-associated DNFB. Tolerance to contact sensitization with DNFB, a T cell-dependent phenomenon, was induced in mice by preparations of DNFB coupled to mouse RBC or spleen cells. Such tolerance is dose related, wanes with time, and can be transferred to normal animals with lymphoid cells (presumably containing suppressors). Tolerance to DNFB-RBC can be produced by whole DNFB-RBC, by ghosts of these cells, by sonicates of the ghosts, and by detergent-treated DNFB-RBC ghosts. Tolerance cannot be produced by larger amounts of DNFB-RBC components not associated with membrane. The ability of various DNP compounds to stimulate DNA synthesis in DNFB-sensitized cells also correlates with their ability to bind to protein components; i.e., DNFB is a far more efficient stimulator than DNBSO, whereas DNPlysine does not stimulate at all. Thus, the ability to sensitize or to tolerize with DNFB congeners is related to their ability to couple to proteins. It appears that the active induction of T cell tolerance requires that tolerogen be coupled to cell membranes. Since both T cell sensitization and tolerance to DNFB are best produced by DNFB-membrane, the actual occurrence of one state or the other must depend on the molecular method of \"presentation\" of DNFB-membrane."}
{"id": "PMID:1084900", "title": "Rat T lymphocyte-specific antigens and their cross-reactivity with mouse T cells.", "content": "Anti-rat T lymphocyte serum (ATLS)2 was prepared by immunizing rabbits with purified T cells from rat mesenteric nodes and absorbed with rat red cells and syngeneic sarcoma cells. The specificity of ATLS for rat T cells was confirmed by the following reasons: a) ATLS was not toxic for bone marrow cells but lysed most of the thymocytes and a number of spleen and lymph node cells, which were inversely correlated to the percentage of cells with B cell characteristics in respective organs; b) anatomical localization of ATLS-reactive cells in lymphoid organs coincided to the thymus-dependent areas, i.e. the paracortex of lymph node and the periarteriolar region of spleen; c) spleen cells treated with ATLS and complement failed to respond to phytohemagglutinin but normally responded to bacterial lipopolysaccharide; d) those cells treated with ATLS and complement could not induce a graft-vs-host reaction in F1 hosts, whereas the same treatment did not affect direct plaque-forming cells. All of these data confirm the specificity of ATLS and indicate that ATLS recognizes rat T lymphocyte-specific antigens (RTLA). Absorption studies showed that RTLA were present in higher concentration on medullary thymocytes and peripheral T cells than on cortical thymocytes, but absent from bone marrow, liver, and brain tissues. When the cross-reactivity of RTLA with mouse T cells was studied by C-dependent cytotoxicity and immunofluorescence, it was found that mouse T cells shared at least one determinant of RTLA with rat T cells, and that distribution pattern of the cross-reacting antigens in mouse lymphoid tissues was essentially the same as that of RTLA in rat lymphoid organs.", "contents": "Rat T lymphocyte-specific antigens and their cross-reactivity with mouse T cells. Anti-rat T lymphocyte serum (ATLS)2 was prepared by immunizing rabbits with purified T cells from rat mesenteric nodes and absorbed with rat red cells and syngeneic sarcoma cells. The specificity of ATLS for rat T cells was confirmed by the following reasons: a) ATLS was not toxic for bone marrow cells but lysed most of the thymocytes and a number of spleen and lymph node cells, which were inversely correlated to the percentage of cells with B cell characteristics in respective organs; b) anatomical localization of ATLS-reactive cells in lymphoid organs coincided to the thymus-dependent areas, i.e. the paracortex of lymph node and the periarteriolar region of spleen; c) spleen cells treated with ATLS and complement failed to respond to phytohemagglutinin but normally responded to bacterial lipopolysaccharide; d) those cells treated with ATLS and complement could not induce a graft-vs-host reaction in F1 hosts, whereas the same treatment did not affect direct plaque-forming cells. All of these data confirm the specificity of ATLS and indicate that ATLS recognizes rat T lymphocyte-specific antigens (RTLA). Absorption studies showed that RTLA were present in higher concentration on medullary thymocytes and peripheral T cells than on cortical thymocytes, but absent from bone marrow, liver, and brain tissues. When the cross-reactivity of RTLA with mouse T cells was studied by C-dependent cytotoxicity and immunofluorescence, it was found that mouse T cells shared at least one determinant of RTLA with rat T cells, and that distribution pattern of the cross-reacting antigens in mouse lymphoid tissues was essentially the same as that of RTLA in rat lymphoid organs."}
{"id": "PMID:1084901", "title": "Isolation of a murine leukemia FC receptor by selective release induced by surface redistribution.", "content": "A protein which binds to the Fc region of IgG has been isolated from the murine leukemia L1210. The isolation technique involves surface cross-linking of the cells's Fc receptors with the use of aggregated human IgG and anti-human IgG. This results in the redistribution (patch formation and capping) of the cells's Fc receptors. Lactoperoxidase-catalyzed radioiodination of the cells before complex binding indicates that Fc receptor redistribution results in the selective release of surface proteins. SDS-PAGE analyses of the supernatants from cells thus treated reveals a major peak corresponding to a molecular weight of 45,000 daltons. This protein has been purified from the cell supernatants by immunoprecipitation and chromatography of the percipitates on Sephadex G-200 under dissociating conditions. After separation from the immune complex this protein can be bound to heat-aggregated IgG, but not aggregated F(ab')2 fragments. The 45,000 dalton protein appears to be the Fc receptor which has been released from the cell surface in association with the complex.", "contents": "Isolation of a murine leukemia FC receptor by selective release induced by surface redistribution. A protein which binds to the Fc region of IgG has been isolated from the murine leukemia L1210. The isolation technique involves surface cross-linking of the cells's Fc receptors with the use of aggregated human IgG and anti-human IgG. This results in the redistribution (patch formation and capping) of the cells's Fc receptors. Lactoperoxidase-catalyzed radioiodination of the cells before complex binding indicates that Fc receptor redistribution results in the selective release of surface proteins. SDS-PAGE analyses of the supernatants from cells thus treated reveals a major peak corresponding to a molecular weight of 45,000 daltons. This protein has been purified from the cell supernatants by immunoprecipitation and chromatography of the percipitates on Sephadex G-200 under dissociating conditions. After separation from the immune complex this protein can be bound to heat-aggregated IgG, but not aggregated F(ab')2 fragments. The 45,000 dalton protein appears to be the Fc receptor which has been released from the cell surface in association with the complex."}
{"id": "PMID:1084902", "title": "T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera.", "content": "The recent development of a reliable murine T lymphocyte proliferation assay has facilitated the study of T lymphocyte function in vitro. In this paper, the effect of anti-histocompatibility antisera on the proliferative response was investigated. The continuous presence of anti-Ia antisera in the cultures was found to inhibit the responses to the antigens poly (Glu58 Lys38 Tyr4) [GLT], poly (Tyr, Glu) ploy D,L Ala-poly Lys [(T,G)-A--L], poly (Phe, Glu)-poly D,L Ala-poly Lys [(phi, G)-A--L], lactate dehydrogenase H4, staphylococcal nuclease, and the IgA myeloma protein, TEPC 15. The T lymphocyte proliferative responses to all of these antigens have previously been shown to be under the genetic control of major histocompatibility-linked immune response genes. The anti-Ia antisera were also capable of inhibiting proliferative responses to antigens such as PPD, to which all strains respond. In contrast, antisera directed solely against H-2K or H-2D antigens did not give significant inhibition. Anti-Ia antisera capable of reacting with antigens coded for by genetically defined subregions of the I locus were capable of completely inhibiting the proliferative response. In the two cases studied, GLT and (T,G)-A--L, an Ir gene controlling the T lymphocyte proliferative response to the antigen had been previously mapped to the same subregion as that which coded for the Ia antigens recognized by the blocking antisera. Finally, in F1 hybrids between responder and nonresponder strains, the anti-Ia antisera showed haplotype-specific inhibition. That is, anti-Ia antisera directed against the responder haplotype could completely block the antigen response controlled by Ir genes of that haplotype; anti-Ia antisera directed against Ia antigens of the nonresponder haplotype gave only partial or no inhibition. Since this selective inhibition was reciprocal depending on which antigen was used, it suggested that the mechanism of anti-Ia antisera inhibition was not cell killing or a nonspecific turning off of the cell but rather a blockade of antigen stimulation at the cell surface. Furthermore, the selective inhibition demonstrates a phenotypic linkage between Ir gene products and Ia antigens at the cell surface. These results, coupled with the known genetic linkage of Ir genes and the genes coding for Ia antigens, suggest that Ia antigens are determinants on Ir gene products.", "contents": "T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera. The recent development of a reliable murine T lymphocyte proliferation assay has facilitated the study of T lymphocyte function in vitro. In this paper, the effect of anti-histocompatibility antisera on the proliferative response was investigated. The continuous presence of anti-Ia antisera in the cultures was found to inhibit the responses to the antigens poly (Glu58 Lys38 Tyr4) [GLT], poly (Tyr, Glu) ploy D,L Ala-poly Lys [(T,G)-A--L], poly (Phe, Glu)-poly D,L Ala-poly Lys [(phi, G)-A--L], lactate dehydrogenase H4, staphylococcal nuclease, and the IgA myeloma protein, TEPC 15. The T lymphocyte proliferative responses to all of these antigens have previously been shown to be under the genetic control of major histocompatibility-linked immune response genes. The anti-Ia antisera were also capable of inhibiting proliferative responses to antigens such as PPD, to which all strains respond. In contrast, antisera directed solely against H-2K or H-2D antigens did not give significant inhibition. Anti-Ia antisera capable of reacting with antigens coded for by genetically defined subregions of the I locus were capable of completely inhibiting the proliferative response. In the two cases studied, GLT and (T,G)-A--L, an Ir gene controlling the T lymphocyte proliferative response to the antigen had been previously mapped to the same subregion as that which coded for the Ia antigens recognized by the blocking antisera. Finally, in F1 hybrids between responder and nonresponder strains, the anti-Ia antisera showed haplotype-specific inhibition. That is, anti-Ia antisera directed against the responder haplotype could completely block the antigen response controlled by Ir genes of that haplotype; anti-Ia antisera directed against Ia antigens of the nonresponder haplotype gave only partial or no inhibition. Since this selective inhibition was reciprocal depending on which antigen was used, it suggested that the mechanism of anti-Ia antisera inhibition was not cell killing or a nonspecific turning off of the cell but rather a blockade of antigen stimulation at the cell surface. Furthermore, the selective inhibition demonstrates a phenotypic linkage between Ir gene products and Ia antigens at the cell surface. These results, coupled with the known genetic linkage of Ir genes and the genes coding for Ia antigens, suggest that Ia antigens are determinants on Ir gene products."}
{"id": "PMID:1084903", "title": "Cellular specificity of plasmacytoma-induced immunosuppression.", "content": "The pattern of immunodeficiency in plasmacytoma-bearing mice appears to be unique. Mice bearing these tumors exhibit a severe impairment in their ability to mount a primary immune response to thymus-dependent and -independent antigens. However, cell-mediated immune functions in these mice apparently remain intact. Thus, when T cell activity of lymph node cells from plasmacytoma-bearing mice was tested in vivo by sensitization with dinitrofluorobenzene and in vitro by responsiveness to phytohemmagglutinin, allogeneic cells, and dinitrobenzene sulfonate, cell-mediated immunity was found to be normal.", "contents": "Cellular specificity of plasmacytoma-induced immunosuppression. The pattern of immunodeficiency in plasmacytoma-bearing mice appears to be unique. Mice bearing these tumors exhibit a severe impairment in their ability to mount a primary immune response to thymus-dependent and -independent antigens. However, cell-mediated immune functions in these mice apparently remain intact. Thus, when T cell activity of lymph node cells from plasmacytoma-bearing mice was tested in vivo by sensitization with dinitrofluorobenzene and in vitro by responsiveness to phytohemmagglutinin, allogeneic cells, and dinitrobenzene sulfonate, cell-mediated immunity was found to be normal."}
{"id": "PMID:1084904", "title": "T cell requirement for experimental allergic encephalomyelitis induction in the rat.", "content": "The question of whether a cell-mediated or a humoral mechanism initiates EAE in rats sensitized with BP-CFA was investigated. The requirement of T cells for EAE induction was manifested when Tx, irradiated rats were reconstituted with normal lymphoid cells treated with ATS and then injected with BP-CFA. Neither EAE nor antibody was produced, indicating the T cell dependency of BP specific antibody production. More precise information regarding the role of the T cell in the production of EAE was obtained by means of passive transfer of EAE with sensitized lymphocytes. Thus, transfer of lymphoid cells from rats previously sensitized to BP-CFA into Tx, irradiated rats elicited EAE and antibodies to BP. However, no EAE followed when the transferred cells were first depleted of T cells by treatment with ATS. Nevertheless, ATP pretreatment did not depress the levels of antibody to BP produced in the transfer recipients. The latter finding indicates that the cells from animals sensitized 9 days previously were already committed to the production of antibodies to BP. Therefore, a) T cells are absolutely necessary for induction of EAE and b) antibody detected by antigen-binding is not responsible for the pathogenesis of this disease.", "contents": "T cell requirement for experimental allergic encephalomyelitis induction in the rat. The question of whether a cell-mediated or a humoral mechanism initiates EAE in rats sensitized with BP-CFA was investigated. The requirement of T cells for EAE induction was manifested when Tx, irradiated rats were reconstituted with normal lymphoid cells treated with ATS and then injected with BP-CFA. Neither EAE nor antibody was produced, indicating the T cell dependency of BP specific antibody production. More precise information regarding the role of the T cell in the production of EAE was obtained by means of passive transfer of EAE with sensitized lymphocytes. Thus, transfer of lymphoid cells from rats previously sensitized to BP-CFA into Tx, irradiated rats elicited EAE and antibodies to BP. However, no EAE followed when the transferred cells were first depleted of T cells by treatment with ATS. Nevertheless, ATP pretreatment did not depress the levels of antibody to BP produced in the transfer recipients. The latter finding indicates that the cells from animals sensitized 9 days previously were already committed to the production of antibodies to BP. Therefore, a) T cells are absolutely necessary for induction of EAE and b) antibody detected by antigen-binding is not responsible for the pathogenesis of this disease."}
{"id": "PMID:1084905", "title": "Recruitment of effector lymphocytes by initiator lymphocytes. Circulating lymphocytes are trapped in the reacting lymph node.", "content": "In previous studies, we have shown that initiator T lymphocytes (ITL) sensitized in vitro recruit effector T lymphocytes in vivo. When ITL were sensitized against fibroblast antigens in vitro and injected into footpads of syngeneic recipients, they induced enlargement of the draining popliteal lymph node (PLN) and the development there of specific effector lymphocytes of recipient origin. To study the basis of this lymph node response in recruitment, we injected 51Cr-labeled spleen cells i.v. into recipients of sensitized ITL and found that the labeled circulating lymphocytes were trapped in the reacting PLN. The trapping depended on surface properties of the labeled circulating lymphocytes, as revealed by various enzymatic treatments. The trapping process was radiosensitive, both on the part of the trapped lymphocytes and the lymph node-trapping mechanism. Thus, sensitized ITL injected into the hind footpads migrate to the PLN and induce the trapping of circulating recruitable lymphocytes, which either differentiate into or regulate the differentiation of effector T lymphocytes.", "contents": "Recruitment of effector lymphocytes by initiator lymphocytes. Circulating lymphocytes are trapped in the reacting lymph node. In previous studies, we have shown that initiator T lymphocytes (ITL) sensitized in vitro recruit effector T lymphocytes in vivo. When ITL were sensitized against fibroblast antigens in vitro and injected into footpads of syngeneic recipients, they induced enlargement of the draining popliteal lymph node (PLN) and the development there of specific effector lymphocytes of recipient origin. To study the basis of this lymph node response in recruitment, we injected 51Cr-labeled spleen cells i.v. into recipients of sensitized ITL and found that the labeled circulating lymphocytes were trapped in the reacting PLN. The trapping depended on surface properties of the labeled circulating lymphocytes, as revealed by various enzymatic treatments. The trapping process was radiosensitive, both on the part of the trapped lymphocytes and the lymph node-trapping mechanism. Thus, sensitized ITL injected into the hind footpads migrate to the PLN and induce the trapping of circulating recruitable lymphocytes, which either differentiate into or regulate the differentiation of effector T lymphocytes."}
{"id": "PMID:1084912", "title": "A temporal dissociation of energy liberation and high energy phosphate splitting during shortening in frog skeletal muscles.", "content": "Measurements of the time course of high energy phosphate splitting and energy liberation were performed on rapidly shortening Rana pipiens skeletal muscles. In muscles contracting 30 times against small loads (less the 0.02P), the ratio of explained heat + work (H + W) (calculated from the measured high energy phosphate splitting) to observed H + W (from myothermal and mechanical measurements) was 0.68 +/- 0.08 and is in agreement with results obtained in isometric tetani of R. pipiens skeletal muscle. In lightly afterloaded muscles which were tetanized for 0.6a and whose metabolism was arrested at 3.0 s after the beginning of stimulation, a similar ratio of explained H + W to observed H + W was obtained. However, in identical contractions in which metabolism was arrested at 0.5-0.75 s after the beginning of stimulation, the ratio of explained H + W to observed H + W declined significantly to values  ranging from 0.15 to 0.40. These results suggest that rapid shortening at the beginning of contraction induces a delay between energy production and measurable high energy phosphate splitting. This interpretation was tested and confirmed in experiments in which one muscle of a pair contracted isometrically while the other contracted against a small afterload. The afterload and stimulus pattern were arranged so that at the time metabolism was arrested, 0.5 s after the beginning of stimulation, the total energy production by both muscles was the same. Chemical analysis revealed that the isotonically contracting muscle spilt only 25% as much high energy phosphate as did the isometrically contracting muscle.", "contents": "A temporal dissociation of energy liberation and high energy phosphate splitting during shortening in frog skeletal muscles. Measurements of the time course of high energy phosphate splitting and energy liberation were performed on rapidly shortening Rana pipiens skeletal muscles. In muscles contracting 30 times against small loads (less the 0.02P), the ratio of explained heat + work (H + W) (calculated from the measured high energy phosphate splitting) to observed H + W (from myothermal and mechanical measurements) was 0.68 +/- 0.08 and is in agreement with results obtained in isometric tetani of R. pipiens skeletal muscle. In lightly afterloaded muscles which were tetanized for 0.6a and whose metabolism was arrested at 3.0 s after the beginning of stimulation, a similar ratio of explained H + W to observed H + W was obtained. However, in identical contractions in which metabolism was arrested at 0.5-0.75 s after the beginning of stimulation, the ratio of explained H + W to observed H + W declined significantly to values  ranging from 0.15 to 0.40. These results suggest that rapid shortening at the beginning of contraction induces a delay between energy production and measurable high energy phosphate splitting. This interpretation was tested and confirmed in experiments in which one muscle of a pair contracted isometrically while the other contracted against a small afterload. The afterload and stimulus pattern were arranged so that at the time metabolism was arrested, 0.5 s after the beginning of stimulation, the total energy production by both muscles was the same. Chemical analysis revealed that the isotonically contracting muscle spilt only 25% as much high energy phosphate as did the isometrically contracting muscle."}
{"id": "PMID:1084913", "title": "A reexamination of the thermoelastic effect in active striated muscle.", "content": "Isolated Rana pipiens sartorius muscles at 0degreeC were stimulated via their nerves and small stretches or releases applied during the plateau of the isometric tetanus at lo. Extra heat above the isometric maintenance heat was produced during the drop in tension caused by release and, for very small releases (delta less than or equal to 0.5% lo), was completely reabsorbed during tension recovery. The extra heat was always directly proportional to the tension change. Heat absorption proportional to the tension change was also observed during the increase in tension produced by small stretches in the range 0.5% lo less than or equal to deltal less than or equal to 3.0% lo. The mean heat:tension ratio R in seven experiments was -0.0084, which is within the range of values reported previously by Woledge. In addition, it was found that during tension recovery after small releases of 1.0% lo less than or equal to deltal less than or equal to 3.0% lo the \"contractile\" component seems able to shorten about 1% lo without producing shortening heat.", "contents": "A reexamination of the thermoelastic effect in active striated muscle. Isolated Rana pipiens sartorius muscles at 0degreeC were stimulated via their nerves and small stretches or releases applied during the plateau of the isometric tetanus at lo. Extra heat above the isometric maintenance heat was produced during the drop in tension caused by release and, for very small releases (delta less than or equal to 0.5% lo), was completely reabsorbed during tension recovery. The extra heat was always directly proportional to the tension change. Heat absorption proportional to the tension change was also observed during the increase in tension produced by small stretches in the range 0.5% lo less than or equal to deltal less than or equal to 3.0% lo. The mean heat:tension ratio R in seven experiments was -0.0084, which is within the range of values reported previously by Woledge. In addition, it was found that during tension recovery after small releases of 1.0% lo less than or equal to deltal less than or equal to 3.0% lo the \"contractile\" component seems able to shorten about 1% lo without producing shortening heat."}
{"id": "PMID:1084914", "title": "Malignant spread of haemangioblastoma: report on two cases.", "content": "Two cases are described in which, after successful removal of a cerebellar haemangioblastoma followed by several years of freedom from symptoms, there developed a progressive spinal cord compression, leading to death. At necropsy the spinal cords in both cases and the brainstem in one case, were irregularly plastered with haemangioblastoma. Although there was no doubt that malignant spread had occurred from one or more primary tumours, the histology of the tumour tissue was in no way different from that of conventional haemangioblastoma.", "contents": "Malignant spread of haemangioblastoma: report on two cases. Two cases are described in which, after successful removal of a cerebellar haemangioblastoma followed by several years of freedom from symptoms, there developed a progressive spinal cord compression, leading to death. At necropsy the spinal cords in both cases and the brainstem in one case, were irregularly plastered with haemangioblastoma. Although there was no doubt that malignant spread had occurred from one or more primary tumours, the histology of the tumour tissue was in no way different from that of conventional haemangioblastoma."}
{"id": "PMID:1084915", "title": "Microtubules in synapses of the retina.", "content": "Using a new method, microtubules can be seen running up to, and lying in close relationship with, the synaptic ribbons in the outer and inner plexiform layers of the frog retina. In the inner plexiform layer microtubules can be seen running up to the terminal membrane in the non-ribbon synapses. Unlike non-ribbon C.N.S. synapses (frog and rat) processed by the same method. There is no clear association between synaptic vesicles and microtubules in the approach regions.", "contents": "Microtubules in synapses of the retina. Using a new method, microtubules can be seen running up to, and lying in close relationship with, the synaptic ribbons in the outer and inner plexiform layers of the frog retina. In the inner plexiform layer microtubules can be seen running up to the terminal membrane in the non-ribbon synapses. Unlike non-ribbon C.N.S. synapses (frog and rat) processed by the same method. There is no clear association between synaptic vesicles and microtubules in the approach regions."}
{"id": "PMID:1084916", "title": "Electrical properties of frog motoneurons in the in situ spinal cord.", "content": "Electrical properties of the spinal motoneurons of Rana temporaria and R. esculenta were investigated in the in situ spinal cord at 20-22 degrees C by means of intracellular recording and current injection. Input resistance values depended on the method of measurement in a given cell but were generally inversely related to axon conduction velocity. The membrane-potential response to a subthreshold current pulse was composed of at least two exponentials with mean time constants of 2.5 and 20 ms. The membrance potential reached by the peak of a spike depended on the mode of spike initiation and membrane potential. Preceding a suprathreshold depolarization by a hyperpolarizing pulse could delay and eliminate spike initiation, similar to effects reported in certain invertebrate neurons. Antidromic invasion frequently failed in motoneurons of normal resting potential. Antidromic spike components (m,IS, SD) were similar to those of cat motoneurons. The delayed depolarization and the long afterhyperpolarization following an antidromic spike had many properties in common with the analogous afterpotentials of cat motoneurons. The reversal potential of the short afterhyperpolarization occurring immediately after the spike varied with resting potential and could not be used to determine potassium equilibrium potential. Sustained rhythmic firing could be evoked by continuous synaptic drive or long pulses of injected current. The plot of firing rate versus current strength had a substantial linear region. Both steady firing and adaptation properties varied markedly with motoneuron input resistance.", "contents": "Electrical properties of frog motoneurons in the in situ spinal cord. Electrical properties of the spinal motoneurons of Rana temporaria and R. esculenta were investigated in the in situ spinal cord at 20-22 degrees C by means of intracellular recording and current injection. Input resistance values depended on the method of measurement in a given cell but were generally inversely related to axon conduction velocity. The membrane-potential response to a subthreshold current pulse was composed of at least two exponentials with mean time constants of 2.5 and 20 ms. The membrance potential reached by the peak of a spike depended on the mode of spike initiation and membrane potential. Preceding a suprathreshold depolarization by a hyperpolarizing pulse could delay and eliminate spike initiation, similar to effects reported in certain invertebrate neurons. Antidromic invasion frequently failed in motoneurons of normal resting potential. Antidromic spike components (m,IS, SD) were similar to those of cat motoneurons. The delayed depolarization and the long afterhyperpolarization following an antidromic spike had many properties in common with the analogous afterpotentials of cat motoneurons. The reversal potential of the short afterhyperpolarization occurring immediately after the spike varied with resting potential and could not be used to determine potassium equilibrium potential. Sustained rhythmic firing could be evoked by continuous synaptic drive or long pulses of injected current. The plot of firing rate versus current strength had a substantial linear region. Both steady firing and adaptation properties varied markedly with motoneuron input resistance."}
{"id": "PMID:1084917", "title": "Evidence for electrotonic coupling between frog motoneurons in the in situ spinal cord.", "content": "A recurrent EPSP was observed on antidromic stimulation of motoneurons in the in situ spinal cord of Rana temporaria and R. esculenta at 20-22C. The EPSP was finely graded and not refractory following full or partial antidromic spike components in a given neuron. The EPSP amplitude varied in parallel with the antidromic field potential under different conditions, suggesting transmission of the EPSP to the recorded motoneuron depended on invasion of the somadendritic membrane or neighboring motoneurons by the antidromic spike. The latency of the EPSP with respect to antidromic invasion of the local motoneuron pool was too short for the EPSP to be mediated by chemical transmission. It was concluded the EPSP was electrically transmitted between the somadendritic membranes of the motoneurons. Under certain conditions, the EPSP magnitude could be made to vary with membrane potential in a direction opposite to that expected from a chemical EPSP. Dendritic spikes were sometimes associated with the EPSP.", "contents": "Evidence for electrotonic coupling between frog motoneurons in the in situ spinal cord. A recurrent EPSP was observed on antidromic stimulation of motoneurons in the in situ spinal cord of Rana temporaria and R. esculenta at 20-22C. The EPSP was finely graded and not refractory following full or partial antidromic spike components in a given neuron. The EPSP amplitude varied in parallel with the antidromic field potential under different conditions, suggesting transmission of the EPSP to the recorded motoneuron depended on invasion of the somadendritic membrane or neighboring motoneurons by the antidromic spike. The latency of the EPSP with respect to antidromic invasion of the local motoneuron pool was too short for the EPSP to be mediated by chemical transmission. It was concluded the EPSP was electrically transmitted between the somadendritic membranes of the motoneurons. Under certain conditions, the EPSP magnitude could be made to vary with membrane potential in a direction opposite to that expected from a chemical EPSP. Dendritic spikes were sometimes associated with the EPSP."}
{"id": "PMID:1084918", "title": "Direct effect of electrical stimulation on peripheral nerve evoked activity: implications in pain relief.", "content": "Experiments were performed with a peripheral neurostimulator, used clinically for pain relief, on isolated cat cutaneous peripheral nerve to determine the effect of electrical stimulation on components of the compound action potential. The results show that neurostimulation alters the conduction velocity and the amplitude of both the A-alpha and beta and the A-delta waves with the more slowly-conducting A-delta component showing the greatest changes. This direct alteration of peripheral nerve activity distal to the first synapse in the spinal cord might contribute to the mechanism of pain relief.", "contents": "Direct effect of electrical stimulation on peripheral nerve evoked activity: implications in pain relief. Experiments were performed with a peripheral neurostimulator, used clinically for pain relief, on isolated cat cutaneous peripheral nerve to determine the effect of electrical stimulation on components of the compound action potential. The results show that neurostimulation alters the conduction velocity and the amplitude of both the A-alpha and beta and the A-delta waves with the more slowly-conducting A-delta component showing the greatest changes. This direct alteration of peripheral nerve activity distal to the first synapse in the spinal cord might contribute to the mechanism of pain relief."}
{"id": "PMID:1084923", "title": "Sub-miniature end-plate potentials at untreated frog neuromuscular junctions.", "content": "1. Some properties of unusually small spontaneous miniature end-plate potentials (sub-min.e.p.p.s) at untreated end-plates of adult frogs have been examined. 2. These small potentials, which show a fast time course, add a highly skewed component to the normal Gaussian distribution of min.e.p.p. amplitudes. 3. Sub-min.e.p.p.s were not evoked by single nerve stimuli, though their frequency could be raised by tetanic nerve stimulation. 4. When min.e.p.p. rate was raised by addition of lanthanum, submin.e.p.p. frequency was also increased, though by a smaller factor than normal min.e.p.p.s. In contrast, sub-min.e.p.p. frequency was unchanged when min.e.p.p. rates were raised by either ethanol or hypertonic solutions.", "contents": "Sub-miniature end-plate potentials at untreated frog neuromuscular junctions. 1. Some properties of unusually small spontaneous miniature end-plate potentials (sub-min.e.p.p.s) at untreated end-plates of adult frogs have been examined. 2. These small potentials, which show a fast time course, add a highly skewed component to the normal Gaussian distribution of min.e.p.p. amplitudes. 3. Sub-min.e.p.p.s were not evoked by single nerve stimuli, though their frequency could be raised by tetanic nerve stimulation. 4. When min.e.p.p. rate was raised by addition of lanthanum, submin.e.p.p. frequency was also increased, though by a smaller factor than normal min.e.p.p.s. In contrast, sub-min.e.p.p. frequency was unchanged when min.e.p.p. rates were raised by either ethanol or hypertonic solutions."}
{"id": "PMID:1084925", "title": "Re-innervation of twitch and slow muscle fibres of the frog after crushing the motor nerves.", "content": "1. The conduction velocities of individual motor axons innervating twitch and slow muscle fibres of the frog were determined by intracellular recording of junctional potentials elicited by stimulating the motor nerves at two different points. 2. In normal pyriformis muscles twitch and slow fibres were found to be innervated by two distinct populations of motor axons. Twitch fibre axons conducted at 10-18-7 m/sec, while the conduction velocities of slow fibre axons ranged from 0-5 to 5 m/sec (at 7-9 degrees C). The thresholds for electrical stimulation were significantly lower in the fast than in the slow axons population. 3. Following denervation by crushing the sciatic nerve fast  axons which re-innervated the muscle had lower conduction velocities than normal but could still be identified. These lower conduction velocities were measured proximal to the site of the crush and did not recover over a period of 446 days. 4. Fast motor axons regenerated more quickly than slow axons and re-innervated twitch as well as slow muscle fibres non-selectively. About 1 month later slow axons re-established synaptic contacts with slow (and some twitch) muscle fibres. Simultaneous re-innervation by fast and slow motor axons was occasionally observed in slow muscle fibres. Finally, the slow muscle fibres were innervated by slow axons only, while synapses of fast axons could no longer be found in this type of muscle fibre. 5. Action potentials were observed in denervated as well as in re-innervated slow muscle fibres; they disappeared as re-innervation progressed. 6. It is concluded that non-selective re-innervation of slow muscle fibres is present in the frog; it is, however, a transient phenomenon followed by restoration of the original innervation pattern.", "contents": "Re-innervation of twitch and slow muscle fibres of the frog after crushing the motor nerves. 1. The conduction velocities of individual motor axons innervating twitch and slow muscle fibres of the frog were determined by intracellular recording of junctional potentials elicited by stimulating the motor nerves at two different points. 2. In normal pyriformis muscles twitch and slow fibres were found to be innervated by two distinct populations of motor axons. Twitch fibre axons conducted at 10-18-7 m/sec, while the conduction velocities of slow fibre axons ranged from 0-5 to 5 m/sec (at 7-9 degrees C). The thresholds for electrical stimulation were significantly lower in the fast than in the slow axons population. 3. Following denervation by crushing the sciatic nerve fast  axons which re-innervated the muscle had lower conduction velocities than normal but could still be identified. These lower conduction velocities were measured proximal to the site of the crush and did not recover over a period of 446 days. 4. Fast motor axons regenerated more quickly than slow axons and re-innervated twitch as well as slow muscle fibres non-selectively. About 1 month later slow axons re-established synaptic contacts with slow (and some twitch) muscle fibres. Simultaneous re-innervation by fast and slow motor axons was occasionally observed in slow muscle fibres. Finally, the slow muscle fibres were innervated by slow axons only, while synapses of fast axons could no longer be found in this type of muscle fibre. 5. Action potentials were observed in denervated as well as in re-innervated slow muscle fibres; they disappeared as re-innervation progressed. 6. It is concluded that non-selective re-innervation of slow muscle fibres is present in the frog; it is, however, a transient phenomenon followed by restoration of the original innervation pattern."}
{"id": "PMID:1084929", "title": "Relationship of lymphocytotoxic antibodies to lymphopenia and parameters of disease activity in systemic lupus erythematosus.", "content": "Peripheral blood lymphocyte subpopulations determined in 40 patients with systemic lupus erythematosus, revealed significant lymphopenia in comparison to normal controls. The lymphopenia was caused by decreases in the absolute numbers of both T cells and surface immunoglobulin bearing (SIg) cells. There were significant percentage decreases in T cells and normal percentages of SIg cells, resulting in an increased percentage of \"null\" cells. The lymphopenia was significantly greater in patients considered to have active disease on the basis of clinical parameters, and was strongly correlated with lymphocytotoxic antibodies, DNA antibodies, and hypocomplementemia. The lymphocytotoxic antibodies were reactive with both T and B lymphocytes from normal and ill donors, human organs, and various lymphoblastoid cell lines. In 30 patients who were followed for up to 22 months, the lymphopenia was clearly seen to develop during, but not before, periods of clinical disease activity, independent of changes in therapy.", "contents": "Relationship of lymphocytotoxic antibodies to lymphopenia and parameters of disease activity in systemic lupus erythematosus. Peripheral blood lymphocyte subpopulations determined in 40 patients with systemic lupus erythematosus, revealed significant lymphopenia in comparison to normal controls. The lymphopenia was caused by decreases in the absolute numbers of both T cells and surface immunoglobulin bearing (SIg) cells. There were significant percentage decreases in T cells and normal percentages of SIg cells, resulting in an increased percentage of \"null\" cells. The lymphopenia was significantly greater in patients considered to have active disease on the basis of clinical parameters, and was strongly correlated with lymphocytotoxic antibodies, DNA antibodies, and hypocomplementemia. The lymphocytotoxic antibodies were reactive with both T and B lymphocytes from normal and ill donors, human organs, and various lymphoblastoid cell lines. In 30 patients who were followed for up to 22 months, the lymphopenia was clearly seen to develop during, but not before, periods of clinical disease activity, independent of changes in therapy."}
{"id": "PMID:1084930", "title": "Triple helices formed by polyuridylic acid with some amino deoxyadenosine derivatives.", "content": "The stability of helical structures formed by polyuridylic acid with nucleosides and nucleotides derived from adenosine is not significantly affected by replacing hydroxyl groups by hydrogen, amino, or azido functions. Stability is affected by the position of the phosphate group.", "contents": "Triple helices formed by polyuridylic acid with some amino deoxyadenosine derivatives. The stability of helical structures formed by polyuridylic acid with nucleosides and nucleotides derived from adenosine is not significantly affected by replacing hydroxyl groups by hydrogen, amino, or azido functions. Stability is affected by the position of the phosphate group."}
{"id": "PMID:1084931", "title": "Aspects of obstetrical defibrination in West Malaysia.", "content": "Five patients illustrate various aspects of obstetrical defibrination in West Malaysia, resulting from exaggeration of changes in fibrinolytic-coagulation equilibrium that occur at delivery. Hypofibrinogenaemia and fibrinolysis may occur in association or either feature predominate. These patients are from a population in which a variety of genetic and environmental factors may interact, e.g. abnormal haemoglobins, cold agglutinins, viral and other infections, introducing additional complications.", "contents": "Aspects of obstetrical defibrination in West Malaysia. Five patients illustrate various aspects of obstetrical defibrination in West Malaysia, resulting from exaggeration of changes in fibrinolytic-coagulation equilibrium that occur at delivery. Hypofibrinogenaemia and fibrinolysis may occur in association or either feature predominate. These patients are from a population in which a variety of genetic and environmental factors may interact, e.g. abnormal haemoglobins, cold agglutinins, viral and other infections, introducing additional complications."}
{"id": "PMID:1084940", "title": "[Characteristics of changes in the kinin system of blood in hypertensive disease].", "content": "The state of the kinin system of blood was studied in terms of its content of kininogen, prekallikrein, kallikrein inhibitor and the spontaneous esterase activity. The examination was conducted in 10 normal individuals and in 81 hypertensive patients with labile and stable arterial pressure and an uncomplicated course. The studies were conducted at rest, during the transfer into an orthostatic position, and after 15 or 60 minutes of walking. The normals demonstrated an activation of the kinin system that ensued in orthostasis, but was more distinct at walking. A significant correlation was noted between the content of prekallikrein and kininogen in all the states under examination. In cases of labile arterial hypertension an increased activation of the blood kinin system was observed at rest, a lack of its activation at walking, and disorders in the correlation between the content of prekallikrein and kininogen. The observed changes in the kinin system may affect the central haemodynamics and the formation of the hyperkinetic type of circulation.", "contents": "[Characteristics of changes in the kinin system of blood in hypertensive disease]. The state of the kinin system of blood was studied in terms of its content of kininogen, prekallikrein, kallikrein inhibitor and the spontaneous esterase activity. The examination was conducted in 10 normal individuals and in 81 hypertensive patients with labile and stable arterial pressure and an uncomplicated course. The studies were conducted at rest, during the transfer into an orthostatic position, and after 15 or 60 minutes of walking. The normals demonstrated an activation of the kinin system that ensued in orthostasis, but was more distinct at walking. A significant correlation was noted between the content of prekallikrein and kininogen in all the states under examination. In cases of labile arterial hypertension an increased activation of the blood kinin system was observed at rest, a lack of its activation at walking, and disorders in the correlation between the content of prekallikrein and kininogen. The observed changes in the kinin system may affect the central haemodynamics and the formation of the hyperkinetic type of circulation."}
{"id": "PMID:1084941", "title": "Estimation of IgG-antiglobulins in sera of patients with rheumatoid arthritis and chronic liver disorders.", "content": "Antiglobulin factors of the IgG class were measured in human sera by a modified immunoadsorbent-technique involving absorption to and elution from insoluble preparations of horse IgG. 32 mg of horse IgG insolubilized with bisdiazotized benzidine were used for quantitative immunoadsorption of IgG antiglobulins from 100 mul serum. Different IgG-antiglobulin concentrations could be obtained in two control groups differing in age. Elevated IgG-antiglobulins levels were found both in seropositive (mean value 23.21 +/- 7.08 mug/ml serum) and seronegative (mean value 20.06 +/- 8.39 mug/ml serum) RA patients. High values of IgG-antiglobulins were found in chronic liver disorders, particularly in the group of CAH patients (mean value 25.10 +/- 6.1 mug/ml serum).", "contents": "Estimation of IgG-antiglobulins in sera of patients with rheumatoid arthritis and chronic liver disorders. Antiglobulin factors of the IgG class were measured in human sera by a modified immunoadsorbent-technique involving absorption to and elution from insoluble preparations of horse IgG. 32 mg of horse IgG insolubilized with bisdiazotized benzidine were used for quantitative immunoadsorption of IgG antiglobulins from 100 mul serum. Different IgG-antiglobulin concentrations could be obtained in two control groups differing in age. Elevated IgG-antiglobulins levels were found both in seropositive (mean value 23.21 +/- 7.08 mug/ml serum) and seronegative (mean value 20.06 +/- 8.39 mug/ml serum) RA patients. High values of IgG-antiglobulins were found in chronic liver disorders, particularly in the group of CAH patients (mean value 25.10 +/- 6.1 mug/ml serum)."}
{"id": "PMID:1084942", "title": "Uptake, localization and secretagogic action of some biogenic amines in the submandibular and pancreatic glands of the guinea pig.", "content": "The effects of noradrenaline, 5-hydroxytryptamine (5-HT) 5-hydroxyptophan (5-HTP), dopamine, L-DOPA and carbamylcholine on the secretion of amylase from guinea pig submandibular gland and pancreas were studied. Carbamylcholine stimulated both pancreatic and salivary gland amylase release, whereas the biogenic amines elicted amylase secretion only from the submandibular gland. L-DOPA and 5-HTP, the precursor amino acids of dopamine and 5-HT, were without effects. In both glands 5-HT and dopamine were accumlated to a greater extent than their precursor amino acids. The uptake of biogenic amines into acinar cells and their effects on amylase secretion are compared and dicussed in terms of receptor specificity.", "contents": "Uptake, localization and secretagogic action of some biogenic amines in the submandibular and pancreatic glands of the guinea pig. The effects of noradrenaline, 5-hydroxytryptamine (5-HT) 5-hydroxyptophan (5-HTP), dopamine, L-DOPA and carbamylcholine on the secretion of amylase from guinea pig submandibular gland and pancreas were studied. Carbamylcholine stimulated both pancreatic and salivary gland amylase release, whereas the biogenic amines elicted amylase secretion only from the submandibular gland. L-DOPA and 5-HTP, the precursor amino acids of dopamine and 5-HT, were without effects. In both glands 5-HT and dopamine were accumlated to a greater extent than their precursor amino acids. The uptake of biogenic amines into acinar cells and their effects on amylase secretion are compared and dicussed in terms of receptor specificity."}
{"id": "PMID:1084944", "title": "Patient participation in the problem-oriented system: a health care plan.", "content": "The problem-oriented system can be used to encourage the involvement of the patient in his health care. A Health Care Plan is described, the purposes of which include facilitation of provider-patient communication, and emphasis on the shared responsibility of provider and patient in problem solving and health maintenance. The Health Care Plan is not designed to supplant other parts of the system such as the problem list or audit.", "contents": "Patient participation in the problem-oriented system: a health care plan. The problem-oriented system can be used to encourage the involvement of the patient in his health care. A Health Care Plan is described, the purposes of which include facilitation of provider-patient communication, and emphasis on the shared responsibility of provider and patient in problem solving and health maintenance. The Health Care Plan is not designed to supplant other parts of the system such as the problem list or audit."}
{"id": "PMID:1084945", "title": "[Lepromatous leprosy after BCG vaccination].", "content": "The authors describe an eruption of cutaneons nodules after a BCG vaccination. Investigation demonstrated that the lesions were caused by Lepromata. An increased number of B lymphocytes was observed in the bloodstream. Ryphampycin was used with good results.", "contents": "[Lepromatous leprosy after BCG vaccination]. The authors describe an eruption of cutaneons nodules after a BCG vaccination. Investigation demonstrated that the lesions were caused by Lepromata. An increased number of B lymphocytes was observed in the bloodstream. Ryphampycin was used with good results."}
{"id": "PMID:1084946", "title": "The ototoxic effects of ethacrynic acid in man and animals.", "content": "A review of the literature was carried out to summarize the ototoxic effects of ethacrynic acid on man and animal. The effects of this drug on the electrolytes of the endolymphatic and perilymphatic systems, on the cochlear potentials and the auditory cortical-evoked responses, were reviewed. Histological animal studies determined by light and electron microscopy were described. Potentiation of this drug with previous aminoglycoside therapy was discussed. In this paper, a human case report with temporal bone findings was present in addition to the histopathological effects of this drug which were shown in a series of animals receiving this drug over a period of time. Edematous and cellular changes in the stria vascularis, especially with animals receiving large doses of the drug, were described. No permanent alterations in the cochlear potentials resulted from the long-term therapy with ethacrynic acid.", "contents": "The ototoxic effects of ethacrynic acid in man and animals. A review of the literature was carried out to summarize the ototoxic effects of ethacrynic acid on man and animal. The effects of this drug on the electrolytes of the endolymphatic and perilymphatic systems, on the cochlear potentials and the auditory cortical-evoked responses, were reviewed. Histological animal studies determined by light and electron microscopy were described. Potentiation of this drug with previous aminoglycoside therapy was discussed. In this paper, a human case report with temporal bone findings was present in addition to the histopathological effects of this drug which were shown in a series of animals receiving this drug over a period of time. Edematous and cellular changes in the stria vascularis, especially with animals receiving large doses of the drug, were described. No permanent alterations in the cochlear potentials resulted from the long-term therapy with ethacrynic acid."}
{"id": "PMID:1084947", "title": "The simultaneous binaural bithermal: a caloric test utilizing electronystagmography.", "content": "The simultaneous irrigation of both external auditory canals with the bithermal stimulus of Fitzgerald and Hallpike yields responses which demonstrate a greater sensitivity than the alternate irrigation of each ear. The responses were recorded utilizing electronystagmography and were compared to the alternate or regular bithermal performed on the same paitent.", "contents": "The simultaneous binaural bithermal: a caloric test utilizing electronystagmography. The simultaneous irrigation of both external auditory canals with the bithermal stimulus of Fitzgerald and Hallpike yields responses which demonstrate a greater sensitivity than the alternate irrigation of each ear. The responses were recorded utilizing electronystagmography and were compared to the alternate or regular bithermal performed on the same paitent."}
{"id": "PMID:1084950", "title": "Biotransformations with plant tissue cultures.", "content": "Suspension cultures of Catharanthus roseus, Apocynum cannabinum and Conium maculatum were examined for their capacity to transform aniline, anisole, acetanilide, benzoic acid and coumarin. None of the cultures transformed acetanilide but each produced acetanilide when fed aniline. All three cultures converted benzoic acid to its para-hydroxy derivative. Coumarin was selectively hydroxylated at the 7-position by Catharanthus and Conium and anisole was O-demethylated only by older Catharanthus tissue.", "contents": "Biotransformations with plant tissue cultures. Suspension cultures of Catharanthus roseus, Apocynum cannabinum and Conium maculatum were examined for their capacity to transform aniline, anisole, acetanilide, benzoic acid and coumarin. None of the cultures transformed acetanilide but each produced acetanilide when fed aniline. All three cultures converted benzoic acid to its para-hydroxy derivative. Coumarin was selectively hydroxylated at the 7-position by Catharanthus and Conium and anisole was O-demethylated only by older Catharanthus tissue."}
{"id": "PMID:1084954", "title": "[Current aspects of differentiation and treatment of pneumocystis carinii pneumonia (author's transl)].", "content": "There are two types of Pneumocystis carinii pneumonia. One is seen in premature and atrophic infants mainly during the 2nd to 6th month, with massive interstitial proliferation of plasmacells and acute respiratory crisis. The second typ is observed in immunodeficient children and adults with only little or no plasmacell infiltration due to the hypoimmune, hypoergic state. Pentamidine is the drug of choice in the therapy of Pneumocystis carinii pneumonia; in serious cases it will be combined with Pyrimethamine. The cocktail Hydergine + Promethazine proved good as a symptomical drug. There have been reports about recoveries in adults under the treatment with Pyrimethamamine + Sulfamethazole. This combination has been recommended as prophylaxis as well.", "contents": "[Current aspects of differentiation and treatment of pneumocystis carinii pneumonia (author's transl)]. There are two types of Pneumocystis carinii pneumonia. One is seen in premature and atrophic infants mainly during the 2nd to 6th month, with massive interstitial proliferation of plasmacells and acute respiratory crisis. The second typ is observed in immunodeficient children and adults with only little or no plasmacell infiltration due to the hypoimmune, hypoergic state. Pentamidine is the drug of choice in the therapy of Pneumocystis carinii pneumonia; in serious cases it will be combined with Pyrimethamine. The cocktail Hydergine + Promethazine proved good as a symptomical drug. There have been reports about recoveries in adults under the treatment with Pyrimethamamine + Sulfamethazole. This combination has been recommended as prophylaxis as well."}
{"id": "PMID:1084955", "title": "Reversions of two proline-requiring auxotrophs of Haemophilus influenzae by n-methyl-n'-nitro-n-nitrosoguanidine and hydrazine.", "content": "New mutation detection systems are described for Haemophilus influenzae. They involve two independently isolated proline auxotrophs which appear to be mutants at different sites in a proline locus (proB) that is very closely linked to a locus (thd) for thymidine requirement. One of the mutants, proB1, appears to revert to prototrophy only by mutations at the locus. The other, proB2, reverts both by mutation at the locus and by unlinked suppressors. The latter account for about 90% of the reversions induced by MNNG and by HZ. The close linkage of proB to thd was used to distinguish between true revertants and suppressors by a transformation test. A comparison was made between the mutation induction kinetics of the different classes of revertants and mutations to novobiocin resistance with MNNG and HZ. The very different induction kinetics for these two mutagens previously reported for the novobiocin resistance system were also found for the proline systems. There were some differences between the detection systems, however, in the frequency of induced mutation relative to the spontaneous frequency and, in one case, in the form of the induction curve. It is concluded that the major features of the induction curves reflect the amount of damage done to DNA and so are general for all systems, but that there are some features which are locus-or site-specific.", "contents": "Reversions of two proline-requiring auxotrophs of Haemophilus influenzae by n-methyl-n'-nitro-n-nitrosoguanidine and hydrazine. New mutation detection systems are described for Haemophilus influenzae. They involve two independently isolated proline auxotrophs which appear to be mutants at different sites in a proline locus (proB) that is very closely linked to a locus (thd) for thymidine requirement. One of the mutants, proB1, appears to revert to prototrophy only by mutations at the locus. The other, proB2, reverts both by mutation at the locus and by unlinked suppressors. The latter account for about 90% of the reversions induced by MNNG and by HZ. The close linkage of proB to thd was used to distinguish between true revertants and suppressors by a transformation test. A comparison was made between the mutation induction kinetics of the different classes of revertants and mutations to novobiocin resistance with MNNG and HZ. The very different induction kinetics for these two mutagens previously reported for the novobiocin resistance system were also found for the proline systems. There were some differences between the detection systems, however, in the frequency of induced mutation relative to the spontaneous frequency and, in one case, in the form of the induction curve. It is concluded that the major features of the induction curves reflect the amount of damage done to DNA and so are general for all systems, but that there are some features which are locus-or site-specific."}
{"id": "PMID:1084956", "title": "Fixation and loss of hydrazine-induced premutational damage in Haemophilus influenzae.", "content": "Premutational damage induced in Haemophilus influenzae by hydrazine appears to be fixed as final mutation only at replication as judged by the transformation assay. Fixation at replication is independent of the rec1 gene, unlike the case with nitrosocarbaryl. Prior to replication premutational damage induced by hydrazine disappears by an unknown process that is not dependent on the presence of a pyrimidine dimer excision system nor on the rec1 gene. Hydrazine does not produce detectable single-strand breaks or alkali-labile sites in the treated DNA nor gaps in DNA newly synthesized after treatment. In these respects it also differs from nitroso compounds. It is concluded that hydrazine acts to produce and altered base, possibly N(4)-aminocytosine, that produces mutations by mispairing at replication rather than by error-prone repair.", "contents": "Fixation and loss of hydrazine-induced premutational damage in Haemophilus influenzae. Premutational damage induced in Haemophilus influenzae by hydrazine appears to be fixed as final mutation only at replication as judged by the transformation assay. Fixation at replication is independent of the rec1 gene, unlike the case with nitrosocarbaryl. Prior to replication premutational damage induced by hydrazine disappears by an unknown process that is not dependent on the presence of a pyrimidine dimer excision system nor on the rec1 gene. Hydrazine does not produce detectable single-strand breaks or alkali-labile sites in the treated DNA nor gaps in DNA newly synthesized after treatment. In these respects it also differs from nitroso compounds. It is concluded that hydrazine acts to produce and altered base, possibly N(4)-aminocytosine, that produces mutations by mispairing at replication rather than by error-prone repair."}
{"id": "PMID:1084953", "title": "[Use of I125 labelled poly(I). poly(C) for determination of antibodies to double-stranded RNA by immune complex absorption to nitrocellulose filters].", "content": "125I-labeled double-stranded polyribonucleotide complex was used for detection of antibodies to double-stranded RNA in sera from people and immunized animals by the method of immune complex adsorption by the nitrocellulose filters. The technique is simple and sensitive. Antibodies to double-stranded RNA WERE detected in sera from patients with different diseases and from normal individuals. Systemic lupus erythematosus sera contain as a rule higher amounts of antibodies to double-stranded RNA. Often these antibodies were measured together with those directed towards native and denatured DNA. Anti-double-stranded RNA antibodies from sera of immunised animals and patients with systemic lupus erythematosus are highly specific.", "contents": "[Use of I125 labelled poly(I). poly(C) for determination of antibodies to double-stranded RNA by immune complex absorption to nitrocellulose filters]. 125I-labeled double-stranded polyribonucleotide complex was used for detection of antibodies to double-stranded RNA in sera from people and immunized animals by the method of immune complex adsorption by the nitrocellulose filters. The technique is simple and sensitive. Antibodies to double-stranded RNA WERE detected in sera from patients with different diseases and from normal individuals. Systemic lupus erythematosus sera contain as a rule higher amounts of antibodies to double-stranded RNA. Often these antibodies were measured together with those directed towards native and denatured DNA. Anti-double-stranded RNA antibodies from sera of immunised animals and patients with systemic lupus erythematosus are highly specific."}
{"id": "PMID:1084960", "title": "[Role of the ventriculocisternostomy in the treatment of non-communicating hydrocephalus].", "content": "44 ventriculo-cisternostomies were carried out on infants and children who manifested non communicating hydrocephalus. They were performed through perforation of the floor of the 3th ventricle with the aid of a leukotomy trochar which was introduced through a frontal ventriculography burr hole, and then guided under X ray control. The study of this group showed that the operation is benign, although the mortality is not null, and is effective except in the case where non communicating hydrocephalus develops after meningeal haemorrhage of meningitis. The late results seem good, but the time lapse is too short for a final conclusion. The preoperative radio-isotopic cisternography does not unable prediction of the cases which will be failures; it does however sometimes make it possible to foresee those which will be successful. The post-operative radio-isotopic cisternography is useful in checking on the permeability of the shunt.", "contents": "[Role of the ventriculocisternostomy in the treatment of non-communicating hydrocephalus]. 44 ventriculo-cisternostomies were carried out on infants and children who manifested non communicating hydrocephalus. They were performed through perforation of the floor of the 3th ventricle with the aid of a leukotomy trochar which was introduced through a frontal ventriculography burr hole, and then guided under X ray control. The study of this group showed that the operation is benign, although the mortality is not null, and is effective except in the case where non communicating hydrocephalus develops after meningeal haemorrhage of meningitis. The late results seem good, but the time lapse is too short for a final conclusion. The preoperative radio-isotopic cisternography does not unable prediction of the cases which will be failures; it does however sometimes make it possible to foresee those which will be successful. The post-operative radio-isotopic cisternography is useful in checking on the permeability of the shunt."}
{"id": "PMID:1084961", "title": "[Selective ventriculography according to the Corrales technique. Its value in the diagnosis of tumors of the 3d ventricle and posterior fossa].", "content": "The authors relate their experience of selective ventriculography. The methode previously described by CORRALES needs a small gold chain with fine links which can reach by gravity the cavity of the fourth ventricle through the third ventricle and the aqueduct of Sylvius. This small chain is used as a leader for a flexible catheter which permits selective clouding of median ventricular cavities. This technique was used in 18 patients. In one case only, the small chain went down without any difficulty into the cisterna magna. The ventricular system was normal. In the 17 other cases, the progression of the small chain was stopped at some point of the ventricular cavities. In each of those cases, the stopping of the small chain appeared to be linked with the presence of a growth directly obstructing or compressing the ventricular cavities and gave a first clue as to its location. Introduction of the catheter into the aqueduct of Sylvius was the only true difficulty; in 3 cases, anatomical (large massa intermedia) or pathological obstacle (right angle bending of the aqueduct of Sylvius) made it impossible. In those 3 cases a satisfactory selective clouding of the fourth ventricle was nevertheless obtained by injecting the dimer either into the posterior part of the 3rd ventricle or into the upper part of the aqueduct of Sylvius. In all cases the trouble some superpositions due to clouding of lateral ventricles were thus avoided and a tomographic investigation, specially useful for the diagnosis of expanding lesions of the third ventricle and posterior fossa was allowed.", "contents": "[Selective ventriculography according to the Corrales technique. Its value in the diagnosis of tumors of the 3d ventricle and posterior fossa]. The authors relate their experience of selective ventriculography. The methode previously described by CORRALES needs a small gold chain with fine links which can reach by gravity the cavity of the fourth ventricle through the third ventricle and the aqueduct of Sylvius. This small chain is used as a leader for a flexible catheter which permits selective clouding of median ventricular cavities. This technique was used in 18 patients. In one case only, the small chain went down without any difficulty into the cisterna magna. The ventricular system was normal. In the 17 other cases, the progression of the small chain was stopped at some point of the ventricular cavities. In each of those cases, the stopping of the small chain appeared to be linked with the presence of a growth directly obstructing or compressing the ventricular cavities and gave a first clue as to its location. Introduction of the catheter into the aqueduct of Sylvius was the only true difficulty; in 3 cases, anatomical (large massa intermedia) or pathological obstacle (right angle bending of the aqueduct of Sylvius) made it impossible. In those 3 cases a satisfactory selective clouding of the fourth ventricle was nevertheless obtained by injecting the dimer either into the posterior part of the 3rd ventricle or into the upper part of the aqueduct of Sylvius. In all cases the trouble some superpositions due to clouding of lateral ventricles were thus avoided and a tomographic investigation, specially useful for the diagnosis of expanding lesions of the third ventricle and posterior fossa was allowed."}
{"id": "PMID:1084963", "title": "The ascending pharyngeal artery: normal and pathological radioanatomy.", "content": "A radioanatomical study of the ascending pharyngeal artery has been performed. Based on 10 injected specimens of anatomical material and 8 superselective, normal and pathological angiograms. The meningeal role of the ascending pharyngeal artery and its anastomoses with the middle meningeal and/or occipital arteries is emphasized.", "contents": "The ascending pharyngeal artery: normal and pathological radioanatomy. A radioanatomical study of the ascending pharyngeal artery has been performed. Based on 10 injected specimens of anatomical material and 8 superselective, normal and pathological angiograms. The meningeal role of the ascending pharyngeal artery and its anastomoses with the middle meningeal and/or occipital arteries is emphasized."}
{"id": "PMID:1084964", "title": "Posterior fossa, aqueduct and fourth ventricle of the living mouse studied by positive contrast and plain radiography.", "content": "Radiographic techniques have been applied to the measure the size, volume and relationships of the posterior fossa, aqueduct and fourth ventricle in two strains of laboratory mice. Significant differences in the size of posterior fossae between strains were observed. Using ventriculography in the living mouse, the outflow tract of the fourth ventricle was demonstrated, together with a communication that exists between the third ventricle and the subarachnoid space.", "contents": "Posterior fossa, aqueduct and fourth ventricle of the living mouse studied by positive contrast and plain radiography. Radiographic techniques have been applied to the measure the size, volume and relationships of the posterior fossa, aqueduct and fourth ventricle in two strains of laboratory mice. Significant differences in the size of posterior fossae between strains were observed. Using ventriculography in the living mouse, the outflow tract of the fourth ventricle was demonstrated, together with a communication that exists between the third ventricle and the subarachnoid space."}
{"id": "PMID:1084965", "title": "Lymphocyte subpopulations in human cerebrospinal fluid.", "content": "Lymphocyte subpopulations in both human cerebrospinal fluid and peripheral blood were identified and compared by rosette techniques. In patients without neuroaxial disease, the percent distribution of Fc receptor and T-lymphocytes reflected peripheral blood values, although there was a significantly higher percentage of T cells in normal CSF. Alterations in lymphocyte cerebrospinal fluid populations were observed in various systemic and neurologic diseases.", "contents": "Lymphocyte subpopulations in human cerebrospinal fluid. Lymphocyte subpopulations in both human cerebrospinal fluid and peripheral blood were identified and compared by rosette techniques. In patients without neuroaxial disease, the percent distribution of Fc receptor and T-lymphocytes reflected peripheral blood values, although there was a significantly higher percentage of T cells in normal CSF. Alterations in lymphocyte cerebrospinal fluid populations were observed in various systemic and neurologic diseases."}
{"id": "PMID:1084966", "title": "Chronic cerebellar stimulation in cerebral palsy.", "content": "Data are presented for the first 50 patients with cerebral palsy who underwent chronic cerebellar stimulation for symptom alleviation. We observed significant shorter and longer term improvement in spasticity as well as athetosis, speech, and functional status. Continuing increments in improvement were noted as a function of time on stimulation. In many instances, psychometric test scores and behavior also were improved. There was one death in this series. There were no neurologic complications due to cerebellar stimulation. The results of this study warrant the judicious use of cerebellar stimulation for symptomatic and functional relief in cerebral palsy.", "contents": "Chronic cerebellar stimulation in cerebral palsy. Data are presented for the first 50 patients with cerebral palsy who underwent chronic cerebellar stimulation for symptom alleviation. We observed significant shorter and longer term improvement in spasticity as well as athetosis, speech, and functional status. Continuing increments in improvement were noted as a function of time on stimulation. In many instances, psychometric test scores and behavior also were improved. There was one death in this series. There were no neurologic complications due to cerebellar stimulation. The results of this study warrant the judicious use of cerebellar stimulation for symptomatic and functional relief in cerebral palsy."}
{"id": "PMID:1084967", "title": "Acute flexion-extension injury of the neck. Electronystagmographic study of 309 patients.", "content": "Three hundred and nine patients, whose chief complaint was dizziness following a flexion-extension type of acceleration neck injury, were examined in the Neurosensory Laboratory of Temple University Health Sciences Center between 1965 and 1972. Specific attention was devoted to vestibular tests, including a search for latent and positional nystagmus, bithermal calorics, and rotatory tests. All tests were performed with the aid of electronystagmography; latent nystagmus was present in 29 percent of the patients, caloric tests were abnormal in 57 percent, and rotatory tests were abnormal in 51 percent. These findings suggest that many of these patients have a verfiable objective vestibular disturbance as a cause of dizziness, even though the neurologic examination may be normal. Furthermore, in order to detect this objective vestibular dysfunction, the patient should be tested with aid of electronystagmography since nystagmus may be inhibited by visual fixation.", "contents": "Acute flexion-extension injury of the neck. Electronystagmographic study of 309 patients. Three hundred and nine patients, whose chief complaint was dizziness following a flexion-extension type of acceleration neck injury, were examined in the Neurosensory Laboratory of Temple University Health Sciences Center between 1965 and 1972. Specific attention was devoted to vestibular tests, including a search for latent and positional nystagmus, bithermal calorics, and rotatory tests. All tests were performed with the aid of electronystagmography; latent nystagmus was present in 29 percent of the patients, caloric tests were abnormal in 57 percent, and rotatory tests were abnormal in 51 percent. These findings suggest that many of these patients have a verfiable objective vestibular disturbance as a cause of dizziness, even though the neurologic examination may be normal. Furthermore, in order to detect this objective vestibular dysfunction, the patient should be tested with aid of electronystagmography since nystagmus may be inhibited by visual fixation."}
{"id": "PMID:1084968", "title": "Beneficial effects of serotonin precursors in postanoxic action myoclonus.", "content": "In two patients with postanoxic action myoclonus, L-tryptophan or a monoamine oxidase inhibitor induced a moderate improvement, but L-5-hydroxytryptophan had greater therapeutic effect. Methysergide, a potent blocker of serotonin receptors, consistently induced a marked deterioration in myoclonus. Pretreatment cerebrospinal fluid 5-hydroxyindoleacetic acid levels were reduced significantly in both patients. These findings suggest that postanoxic action myoclonus likely is associated with insufficient serotonergic activity in the central nervous system. Data are inadequate to determine whether this apparent insufficiency reflects structural changes in 5HT-containing raphe nuclei due to a direct anoxic damage to these structures of functional changes caused by a secondary reduction in the activity of intact serotonergic neurons.", "contents": "Beneficial effects of serotonin precursors in postanoxic action myoclonus. In two patients with postanoxic action myoclonus, L-tryptophan or a monoamine oxidase inhibitor induced a moderate improvement, but L-5-hydroxytryptophan had greater therapeutic effect. Methysergide, a potent blocker of serotonin receptors, consistently induced a marked deterioration in myoclonus. Pretreatment cerebrospinal fluid 5-hydroxyindoleacetic acid levels were reduced significantly in both patients. These findings suggest that postanoxic action myoclonus likely is associated with insufficient serotonergic activity in the central nervous system. Data are inadequate to determine whether this apparent insufficiency reflects structural changes in 5HT-containing raphe nuclei due to a direct anoxic damage to these structures of functional changes caused by a secondary reduction in the activity of intact serotonergic neurons."}
{"id": "PMID:1084996", "title": "Ontogeny of mouse B lymphocytes and inactivation by antigen of early B lymphocytes.", "content": "Taking advantage of recent findings about membrane fluidity, we have studied and compared the biosynthetic capacities of fetal or neonatal mouse B (bone-marrow derived) lymphocytes (until 10 days after birth) and adult B lymphocytes. Although both early and adult lymphocytes can synthesize surface immunoglobulins, they have a different physiological behavior after interaction with a ligand (anti-immunoglobulin sera or antigen), either in vivo or in vitro. Fetal and neonatal lymphocytes bearing surface immunoglobulins do not reexpress their membrane receptors after capping and endocytosis promoted by anti-immunoglobulin sera. On the other hand, adult lymphocytes resynthesize completely their receptors after the same treatment. Furthermore, intrafetal injections of hemocyanin in pregnant mice lead to a striking decrease in the number of hemocyanin-binding cells. It seems plausible that this non-reexpression of surface immunoglobulins could be the first step in tolerance establishment.", "contents": "Ontogeny of mouse B lymphocytes and inactivation by antigen of early B lymphocytes. Taking advantage of recent findings about membrane fluidity, we have studied and compared the biosynthetic capacities of fetal or neonatal mouse B (bone-marrow derived) lymphocytes (until 10 days after birth) and adult B lymphocytes. Although both early and adult lymphocytes can synthesize surface immunoglobulins, they have a different physiological behavior after interaction with a ligand (anti-immunoglobulin sera or antigen), either in vivo or in vitro. Fetal and neonatal lymphocytes bearing surface immunoglobulins do not reexpress their membrane receptors after capping and endocytosis promoted by anti-immunoglobulin sera. On the other hand, adult lymphocytes resynthesize completely their receptors after the same treatment. Furthermore, intrafetal injections of hemocyanin in pregnant mice lead to a striking decrease in the number of hemocyanin-binding cells. It seems plausible that this non-reexpression of surface immunoglobulins could be the first step in tolerance establishment."}
{"id": "PMID:1084997", "title": "Role of major histocompatibility complex gene products in delayed-type hypersensitivity.", "content": "Sensitized thymus-derived (T) lymphocytes can transfer delayed-type hypersensitivity (DTH) to naive mice only if there is identity at the major histocompatibility complex (MHC). The MHC region responsible differs according to the antigen used for sensitization. For transfer of DTH to fowl gamma globulin identity at I-A is necessary; for dinitrofluorobenzene, however, identity at either K, D, or I region is sufficient. T cells of one genotype, sensitized in a chimeric environment, transferred DTH to both parental strains even though these were MHC incompatible. However T cells from F1 hybrid mice, sensitized not in the F1 but in one parental strain, transferred DTH only to that parental strain, not to the other, in contrast to F1 T cells sensitized in the F1 which could transfer DTH to both parental strains. Macrophages pulsed with antigen in vitro could be used to sensitize syngeneic or semi-allogeneic mice for the transfer of DTH. Transfer was, however, successful only in the strain syngeneic to that from which the macrophages were derived. Evidence is also presented that genetically low-responder mice can be made to exhibit DTH provided they are pretreated with cyclophosphamide two days before sensitization. When considered in toto these results strongly argue in favor of the notion that there are receptors on activated T cells which recognize antigenic determinants and MHC gene products. The implications of these findings are discussed in relation to the role of macrophages in antigen presentation and to the possible parallel evolution of MHC gene products and of T cell receptors for antigen.", "contents": "Role of major histocompatibility complex gene products in delayed-type hypersensitivity. Sensitized thymus-derived (T) lymphocytes can transfer delayed-type hypersensitivity (DTH) to naive mice only if there is identity at the major histocompatibility complex (MHC). The MHC region responsible differs according to the antigen used for sensitization. For transfer of DTH to fowl gamma globulin identity at I-A is necessary; for dinitrofluorobenzene, however, identity at either K, D, or I region is sufficient. T cells of one genotype, sensitized in a chimeric environment, transferred DTH to both parental strains even though these were MHC incompatible. However T cells from F1 hybrid mice, sensitized not in the F1 but in one parental strain, transferred DTH only to that parental strain, not to the other, in contrast to F1 T cells sensitized in the F1 which could transfer DTH to both parental strains. Macrophages pulsed with antigen in vitro could be used to sensitize syngeneic or semi-allogeneic mice for the transfer of DTH. Transfer was, however, successful only in the strain syngeneic to that from which the macrophages were derived. Evidence is also presented that genetically low-responder mice can be made to exhibit DTH provided they are pretreated with cyclophosphamide two days before sensitization. When considered in toto these results strongly argue in favor of the notion that there are receptors on activated T cells which recognize antigenic determinants and MHC gene products. The implications of these findings are discussed in relation to the role of macrophages in antigen presentation and to the possible parallel evolution of MHC gene products and of T cell receptors for antigen."}
{"id": "PMID:1085003", "title": "Diverticular disease.", "content": "Diverticular disease comprises a spectrum of illness beginning with the irritable bowel syndrome and progressing to the life-threatening complications of diverticulitis and hemorrhage. Step-wise progression of this disease may be seen but is not invariably present; many patients with diverticulosis do not have preceding symptoms of the irritable bowel syndrome. The typical complaints of irregularity of bowel habits and abdominal pain will usually respond to the relatively new treatment modality of a high fiber diet with added wheat bran. Barium enema x-ray examination remains the primary diagnostic modality in the investigation of diverticular disease, and colonoscopy should be used only in the presence of certain specific circumstances. Surgery, aimed at the treatment of complications of this disease, has progressed to the point where one-stage extirpation of the diseased bowel is recommended.", "contents": "Diverticular disease. Diverticular disease comprises a spectrum of illness beginning with the irritable bowel syndrome and progressing to the life-threatening complications of diverticulitis and hemorrhage. Step-wise progression of this disease may be seen but is not invariably present; many patients with diverticulosis do not have preceding symptoms of the irritable bowel syndrome. The typical complaints of irregularity of bowel habits and abdominal pain will usually respond to the relatively new treatment modality of a high fiber diet with added wheat bran. Barium enema x-ray examination remains the primary diagnostic modality in the investigation of diverticular disease, and colonoscopy should be used only in the presence of certain specific circumstances. Surgery, aimed at the treatment of complications of this disease, has progressed to the point where one-stage extirpation of the diseased bowel is recommended."}
{"id": "PMID:1085000", "title": "[Electrocolloidotherapy].", "content": "Electro-colloidal therapy opposes the electro-colloidal precipitation that governs or participates in the development of pathological conditions. This therapy is carried out using an apparatus that re-establishes or maintains the correct electrical and colloidal state in the human body. The use of electro-colloidal therapy combined with the usual types of vascular therapy gives results that would not be possible with either type alone. The indication of choice for electro-colloidal therapy is vascular thrombosis. All the 68 cases treated were improved by the electrical treatment. Arteritis and vascular tissue degeneration in general represent the second and more controversial indication for electro-colloidal therapy.", "contents": "[Electrocolloidotherapy]. Electro-colloidal therapy opposes the electro-colloidal precipitation that governs or participates in the development of pathological conditions. This therapy is carried out using an apparatus that re-establishes or maintains the correct electrical and colloidal state in the human body. The use of electro-colloidal therapy combined with the usual types of vascular therapy gives results that would not be possible with either type alone. The indication of choice for electro-colloidal therapy is vascular thrombosis. All the 68 cases treated were improved by the electrical treatment. Arteritis and vascular tissue degeneration in general represent the second and more controversial indication for electro-colloidal therapy."}
{"id": "PMID:1085004", "title": "Alpha-1-antitrypsin deficiency and liver in adults.", "content": "Thirteen adult patients (aged 16 to 73 years) form 12 families are described with liver disease and alpha- 1- antitrypsin deficiency. Long-term observation of several of these patients suggests that the liver disease may be only slowly progressive, but review of possible factors aggravating this has failed to reveal any obvious clues. Progression to death from hepatic failure was the commonest outcome, but one patient developed a malignant hepatoma and two others died because of intraperitoneal haemorrhage due to ruptured cirrhotic nodules--a complication not hitherto described in association with this condtion. Diagnosis of alpha-1-antitrypsin deficiency was based on serological, histological, immunopathological and genetic studies. The most useful screening test in liver disease was found to be the demonstration of PAS positive globules in liver biopsy material which is diagn by immunofluoresence or immunoperoxidase, the latter being a superior technique. Serum estimation of alpha-1 -antitrypsin deficiency was performed by immunoelectropharetic and immunodiffusion techniques, the former being preferred because it gave more consistent results. Both methods, however, were of limited value since wide variations in the serum values are commonly found in normal and abnormal states. Genotyping was carried out using starch gel electrophoresis and although of value in family studies, its value as a diagnositc aid is limited because of technical difficulties and also because alpha-1-antitrypsin accumulation in the liver may be found in both homozygous and heterozygous states. It is suggested that adult liver disease associated with abnormalities in alpha-1-antitrypsin may be more common than has hitherto been reported. This condition should be systematically sought in all cases of liver disease of uncertain aetiology.", "contents": "Alpha-1-antitrypsin deficiency and liver in adults. Thirteen adult patients (aged 16 to 73 years) form 12 families are described with liver disease and alpha- 1- antitrypsin deficiency. Long-term observation of several of these patients suggests that the liver disease may be only slowly progressive, but review of possible factors aggravating this has failed to reveal any obvious clues. Progression to death from hepatic failure was the commonest outcome, but one patient developed a malignant hepatoma and two others died because of intraperitoneal haemorrhage due to ruptured cirrhotic nodules--a complication not hitherto described in association with this condtion. Diagnosis of alpha-1-antitrypsin deficiency was based on serological, histological, immunopathological and genetic studies. The most useful screening test in liver disease was found to be the demonstration of PAS positive globules in liver biopsy material which is diagn by immunofluoresence or immunoperoxidase, the latter being a superior technique. Serum estimation of alpha-1 -antitrypsin deficiency was performed by immunoelectropharetic and immunodiffusion techniques, the former being preferred because it gave more consistent results. Both methods, however, were of limited value since wide variations in the serum values are commonly found in normal and abnormal states. Genotyping was carried out using starch gel electrophoresis and although of value in family studies, its value as a diagnositc aid is limited because of technical difficulties and also because alpha-1-antitrypsin accumulation in the liver may be found in both homozygous and heterozygous states. It is suggested that adult liver disease associated with abnormalities in alpha-1-antitrypsin may be more common than has hitherto been reported. This condition should be systematically sought in all cases of liver disease of uncertain aetiology."}
{"id": "PMID:1085001", "title": "[Pitfalls during phlebology consultation].", "content": "For a patient suffering with his legs, the pain is usually associated with the presence of one or more varices that can be seen. The physician must not fall into this trap. Questioning is vital. Establishing the kind of pain and the conditions under which it occurs may elok for (1) a rheumatological etioloty: sciatic or crural nerve pain, calcaneal pain: (2) a surgical etiology: an inguinal or particularly a crural hernia, a popliteal cyst; (3) a medical etiology: exogenous polyneuritis, hypokalaemia resulting from the abuse of laxatives or of diuretics, etc.", "contents": "[Pitfalls during phlebology consultation]. For a patient suffering with his legs, the pain is usually associated with the presence of one or more varices that can be seen. The physician must not fall into this trap. Questioning is vital. Establishing the kind of pain and the conditions under which it occurs may elok for (1) a rheumatological etioloty: sciatic or crural nerve pain, calcaneal pain: (2) a surgical etiology: an inguinal or particularly a crural hernia, a popliteal cyst; (3) a medical etiology: exogenous polyneuritis, hypokalaemia resulting from the abuse of laxatives or of diuretics, etc."}
{"id": "PMID:1085005", "title": "Fibrogenesis imperfecta ossium.", "content": "The case history, clinical course and laboratory findings in a 66-year-old woman with fibrogenesis imperfecta ossium are reported, the sixth case in the literature. The condition is characterized clinically by intractable skeletal pain and progressive immobility. Though serum alkaline phosphatase has been raised in all patients, there are no specific haematological or biochemical findings. The radiological features of coarse and dense trabecular pattern with symmetrical and diffuse involvement of all bones without expansion or change of shape, together with periosteal reactions and soft tissue calcification are characteristic. The macroscopic appearance of bone shows large areas of opaque white and brittle trabeculae. The histological findings mimic those of osteomalacia unless examined under polarized light which shows the loss of normal birefringence. On electron microscopy the normal lamellar pattern made up of orientated collagen fibrils all about 80 nm diameter is replaced by a random tangled pattern of much thinner irregularly curved fibrils, some as thin as 5nm. The condition appears to be acquired, leading to erosion of the normal skeleton and replacement with an abnormal fibre deficient matrix. There is no definitive therapy at present.", "contents": "Fibrogenesis imperfecta ossium. The case history, clinical course and laboratory findings in a 66-year-old woman with fibrogenesis imperfecta ossium are reported, the sixth case in the literature. The condition is characterized clinically by intractable skeletal pain and progressive immobility. Though serum alkaline phosphatase has been raised in all patients, there are no specific haematological or biochemical findings. The radiological features of coarse and dense trabecular pattern with symmetrical and diffuse involvement of all bones without expansion or change of shape, together with periosteal reactions and soft tissue calcification are characteristic. The macroscopic appearance of bone shows large areas of opaque white and brittle trabeculae. The histological findings mimic those of osteomalacia unless examined under polarized light which shows the loss of normal birefringence. On electron microscopy the normal lamellar pattern made up of orientated collagen fibrils all about 80 nm diameter is replaced by a random tangled pattern of much thinner irregularly curved fibrils, some as thin as 5nm. The condition appears to be acquired, leading to erosion of the normal skeleton and replacement with an abnormal fibre deficient matrix. There is no definitive therapy at present."}
{"id": "PMID:1085006", "title": "Clinical and immunological aspects of cutaneous vasculitis.", "content": "Thirty-five patients with cutaneous vasculitis were studied. Sixteen had raised serum levels of cryoglobulins. In all but one the cryoglobulin was an immune complex. Eleven patients had anticomplementary sera and five had a low serum IgM. Clinically, the cryoglobulinaemic patients were not readily distinguishable from the non-cryoglobulinaemic in that cold sensitivity occurred in only six of sixteen patients with cryoglobulins. Peripheral neuropathy and lesions in the upper respiratory tract were confined to those with cryoglobulinaemia. An immunopathological classification for the purpuric vasculitides is suggested.", "contents": "Clinical and immunological aspects of cutaneous vasculitis. Thirty-five patients with cutaneous vasculitis were studied. Sixteen had raised serum levels of cryoglobulins. In all but one the cryoglobulin was an immune complex. Eleven patients had anticomplementary sera and five had a low serum IgM. Clinically, the cryoglobulinaemic patients were not readily distinguishable from the non-cryoglobulinaemic in that cold sensitivity occurred in only six of sixteen patients with cryoglobulins. Peripheral neuropathy and lesions in the upper respiratory tract were confined to those with cryoglobulinaemia. An immunopathological classification for the purpuric vasculitides is suggested."}
{"id": "PMID:1085007", "title": "Neuroradiological applications of the Ommaya reservoir system.", "content": "By way of a frontal burr hole a subcutaneous reservoir connected to a flexible catheter inserted through a foramen of Monroe into the third ventricle is installed. The use of this device facilitates contrast and radioisotope ventriculography, reducing both trauma and stress to the patient at the same time. The procedure greatly improves the quality of contrast studies in space-occupying lesions in the posterior fossa by rendering simultaneous cisternography technically possible and safe.", "contents": "Neuroradiological applications of the Ommaya reservoir system. By way of a frontal burr hole a subcutaneous reservoir connected to a flexible catheter inserted through a foramen of Monroe into the third ventricle is installed. The use of this device facilitates contrast and radioisotope ventriculography, reducing both trauma and stress to the patient at the same time. The procedure greatly improves the quality of contrast studies in space-occupying lesions in the posterior fossa by rendering simultaneous cisternography technically possible and safe."}
{"id": "PMID:1085008", "title": "Transcatheter therapeutic embolization in the management of massive upper gastrointestinal bleeding.", "content": "Two methods of controlling life-threatening hemorrhage are infusion of vasospastic agents and embolization of the bleeding vessel. Gelfoam powder, Gelfoam sponge, and isobutyl cyanoacrylate have been used to control severe arterial and variceal hemorrhage from the upper gastrointestinal tract. The authors summarize their results and discuss gastric necrosis, a serious complication.", "contents": "Transcatheter therapeutic embolization in the management of massive upper gastrointestinal bleeding. Two methods of controlling life-threatening hemorrhage are infusion of vasospastic agents and embolization of the bleeding vessel. Gelfoam powder, Gelfoam sponge, and isobutyl cyanoacrylate have been used to control severe arterial and variceal hemorrhage from the upper gastrointestinal tract. The authors summarize their results and discuss gastric necrosis, a serious complication."}
{"id": "PMID:1085009", "title": "Transcatheter therapeutic arterial embolization.", "content": "Transcatheter therapeutic arterial embolization was employed in 11 patients with gastrointestinal hemorrhage and 8 patients with bleeding from other sites. Hemorrhage was stopped successfully in all of the patients with gastrointestinal bleeding and 6 of the 7 patients with active bleeding from other sites. There were no significant complications or sequelae, although ischemia msy cause problems in such patients. Embolization is of considerable value when pharmacological therapy fails or is not appropriate due to the site of bleeding. In some cases it may represent the definitive treatment, as in pelvic trauma or renal hemorrhage; in others, embolization may serve to halt bleeding long enough to permit surgery. Therapeutic embolization should be considered whenever active extravasation of contrast material is demonstrated.", "contents": "Transcatheter therapeutic arterial embolization. Transcatheter therapeutic arterial embolization was employed in 11 patients with gastrointestinal hemorrhage and 8 patients with bleeding from other sites. Hemorrhage was stopped successfully in all of the patients with gastrointestinal bleeding and 6 of the 7 patients with active bleeding from other sites. There were no significant complications or sequelae, although ischemia msy cause problems in such patients. Embolization is of considerable value when pharmacological therapy fails or is not appropriate due to the site of bleeding. In some cases it may represent the definitive treatment, as in pelvic trauma or renal hemorrhage; in others, embolization may serve to halt bleeding long enough to permit surgery. Therapeutic embolization should be considered whenever active extravasation of contrast material is demonstrated."}
{"id": "PMID:1085010", "title": "Alterations in gastric physiology caused by selective embolization and vasopressin infusion of the left gastric artery.", "content": "Alterations in gastric physiology caused by selective embolization and vasopressin infusion of the left gastric artery were evaluated in 29 dogs. Gastric acidity was not significantly altered following Gelfoam embolization but decreased sharply with vasopressin infusion. These results suggest that the segmental occlusion caused by Gelfoam embolization permits significant collateral blood flow to the gastric mucosa, while the arteriolar and capillary constriction caused by vasopressin effectively decreases mucosal blood flow. These findings are consistent with the clincal observation that embolization is more effective in controlling bleeding ulcers, while vasopressin infusion is more effective for controlling hemorrhagic gastritis.", "contents": "Alterations in gastric physiology caused by selective embolization and vasopressin infusion of the left gastric artery. Alterations in gastric physiology caused by selective embolization and vasopressin infusion of the left gastric artery were evaluated in 29 dogs. Gastric acidity was not significantly altered following Gelfoam embolization but decreased sharply with vasopressin infusion. These results suggest that the segmental occlusion caused by Gelfoam embolization permits significant collateral blood flow to the gastric mucosa, while the arteriolar and capillary constriction caused by vasopressin effectively decreases mucosal blood flow. These findings are consistent with the clincal observation that embolization is more effective in controlling bleeding ulcers, while vasopressin infusion is more effective for controlling hemorrhagic gastritis."}
{"id": "PMID:1085012", "title": "Effects of beta-carbolines of plasma corticosterone in mice.", "content": "6-Methoxy-1, 2, 3, 4-tetrahydro-beta-carboline, a drug which has been reported to increase brain serotonin (5-HT) but not norepinephrine, was tested for its acute effects of pituitary-adrenocortical activity in CF1 mice. The drug produced dose- and time-dependent increases in plasma corticosterone concentration which could be prevented by blocking adrenocorticotropic hormone secretion. Administration of other beta-carbolines or the 5-HT precursor 5-hydroxytryptophan also resulted in plasma corticosterone elevations. Further studies will be needed to determine whether beta-carbolines stimulate pituitary-adrenocortical activity through a central serotonergic system or by some alternate mechanism.", "contents": "Effects of beta-carbolines of plasma corticosterone in mice. 6-Methoxy-1, 2, 3, 4-tetrahydro-beta-carboline, a drug which has been reported to increase brain serotonin (5-HT) but not norepinephrine, was tested for its acute effects of pituitary-adrenocortical activity in CF1 mice. The drug produced dose- and time-dependent increases in plasma corticosterone concentration which could be prevented by blocking adrenocorticotropic hormone secretion. Administration of other beta-carbolines or the 5-HT precursor 5-hydroxytryptophan also resulted in plasma corticosterone elevations. Further studies will be needed to determine whether beta-carbolines stimulate pituitary-adrenocortical activity through a central serotonergic system or by some alternate mechanism."}
{"id": "PMID:1085024", "title": "[Contribution of axial computer assisted tomography to the diagnosis of aresorptive hydrocephalus].", "content": "In a comparative study, 18 patients presenting with progressive mental deterioration were investigated by means of radio-isotopic cisternography and by computerized axial tomography (CAT) in order to establish morphological criteria for CAT which would enable malresorptive hydrocephalus and atrophic hydrocephalus to be differentiated. Except for one case, malresorptive hydrocephalus was characterized by supraand infratentorial ventricular dilatation without any signs of cortical atrophy of the convexity. In most cases, ventricular dilatation was more important in cases of malresorptive hydrocephalus and, in 6 out of 7 cases, the trigone and the occipital horns were particularly dilated. Only in malresorptive hydrocephalus did we find less than juxtaventricular aedema greater than possibly indicating compensating transependymal absorption of cerebrospinal fluid. In atrophic hydrocephalus, on the other hand, CAT shows a moderate degree of ventricular dilatation associated with dilatation of the cortical sulci.", "contents": "[Contribution of axial computer assisted tomography to the diagnosis of aresorptive hydrocephalus]. In a comparative study, 18 patients presenting with progressive mental deterioration were investigated by means of radio-isotopic cisternography and by computerized axial tomography (CAT) in order to establish morphological criteria for CAT which would enable malresorptive hydrocephalus and atrophic hydrocephalus to be differentiated. Except for one case, malresorptive hydrocephalus was characterized by supraand infratentorial ventricular dilatation without any signs of cortical atrophy of the convexity. In most cases, ventricular dilatation was more important in cases of malresorptive hydrocephalus and, in 6 out of 7 cases, the trigone and the occipital horns were particularly dilated. Only in malresorptive hydrocephalus did we find less than juxtaventricular aedema greater than possibly indicating compensating transependymal absorption of cerebrospinal fluid. In atrophic hydrocephalus, on the other hand, CAT shows a moderate degree of ventricular dilatation associated with dilatation of the cortical sulci."}
{"id": "PMID:1085030", "title": "A colchicine-sensitivity test for leukaemic lymphocytes.", "content": "Non-dividing lymphocytes from patients with chronic lymphocytic leukaemia were more sensitive than normal lymphocytes to reagents as prednisolone, cytarabine, vincristine and colchicine. The maximum difference was obtained when the cells were incubated with colchicine at 37 degrees C for 20 h. The sensitivity was measured by a 'sensitivity index' which was an estimate of the average percentage of lymphocytes killed by 1.0 and 0.1 mug/ml of colchicine. The index was 0-15% for lymphocytes from the blood of 14 normal persons and was 61-98% for 23 of 25 leukaemic patients with absolute lymphocyte counts of 8,000 X 10(9)/l or more. 3 of 4 untreated patients with presumptive diagnoses of early leukaemia had low absolute counts of 3,300 to 7,600 X 10(9) lymphocytes/l and high sensitivity indices of 41 to 83%. Tests on treated patients with lymphocytes counts less than 8,000 X 10(9)/l suggested a correlation of the index with remission and relapse. Hairy cells from 3 patients with hairy cell leukaemia were resistant to colchicine. Sensitivity to colchicine seemed useful at a test for leukaemic lymphocytes and as an aid in the haematologic evaluation of patients with chronic lymphocytic leukaemia and malignant lymphoma.", "contents": "A colchicine-sensitivity test for leukaemic lymphocytes. Non-dividing lymphocytes from patients with chronic lymphocytic leukaemia were more sensitive than normal lymphocytes to reagents as prednisolone, cytarabine, vincristine and colchicine. The maximum difference was obtained when the cells were incubated with colchicine at 37 degrees C for 20 h. The sensitivity was measured by a 'sensitivity index' which was an estimate of the average percentage of lymphocytes killed by 1.0 and 0.1 mug/ml of colchicine. The index was 0-15% for lymphocytes from the blood of 14 normal persons and was 61-98% for 23 of 25 leukaemic patients with absolute lymphocyte counts of 8,000 X 10(9)/l or more. 3 of 4 untreated patients with presumptive diagnoses of early leukaemia had low absolute counts of 3,300 to 7,600 X 10(9) lymphocytes/l and high sensitivity indices of 41 to 83%. Tests on treated patients with lymphocytes counts less than 8,000 X 10(9)/l suggested a correlation of the index with remission and relapse. Hairy cells from 3 patients with hairy cell leukaemia were resistant to colchicine. Sensitivity to colchicine seemed useful at a test for leukaemic lymphocytes and as an aid in the haematologic evaluation of patients with chronic lymphocytic leukaemia and malignant lymphoma."}
{"id": "PMID:1085031", "title": "Transplantation of bursal stem cells into cyclophosphamide-treated chicks. Lymphoid repopulation of splenic structures.", "content": "After transplantation of bursal stem cells into cyclophoshamide-treated newly hatched chicks, the first lymphoid cells in the spleen of the recipients were observed on the first day after the cell transfer and the first plasma cells on day 3. The repopulation of the Schweigger-Seidel sheaths started from their periphery on day 6. The first germinal centers were detected 20 days after the cell transfer. In cyclophosphamide-treated birds without cell transplantation, only the periphery of the Schweigger-Seidel sheaths became repopulated, and no plasma cells or germinal centers were observed. The results indicate that already at the age of 3 days the bursa contains some cells of postbursal maturity, able to home to the spleen.", "contents": "Transplantation of bursal stem cells into cyclophosphamide-treated chicks. Lymphoid repopulation of splenic structures. After transplantation of bursal stem cells into cyclophoshamide-treated newly hatched chicks, the first lymphoid cells in the spleen of the recipients were observed on the first day after the cell transfer and the first plasma cells on day 3. The repopulation of the Schweigger-Seidel sheaths started from their periphery on day 6. The first germinal centers were detected 20 days after the cell transfer. In cyclophosphamide-treated birds without cell transplantation, only the periphery of the Schweigger-Seidel sheaths became repopulated, and no plasma cells or germinal centers were observed. The results indicate that already at the age of 3 days the bursa contains some cells of postbursal maturity, able to home to the spleen."}
{"id": "PMID:1085032", "title": "Volar tenosynovectomy in the rheumatoid hand.", "content": "Rheumatoid involvement on the flexor aspect of the hand is common, but is easily overlooked because of difficulty in clinical examination. It is our experience that this localization of the disease process is extremely important and that surgical treatment should be given high priority. In this study a series of 235 operations on the flexor aspect of the hand performed on 139 patients are presented. The results are very gratifying as far as tenosynovectomy in the carpal tunnel and the pain is concerned. When performed on the fingers, however, varying degrees of postoperative flexion contractures developed in no less than 44%. It was found that this problem arose when the synovectomy was extended beyond the level of the middle flexion crease of the finger being caused by excessive postoperative scar formation in the region volar to the proximal interphalangeal joint between the tails of the superficial tendon. It is concluded that less extensive surgery should be aimed at in this particular area.", "contents": "Volar tenosynovectomy in the rheumatoid hand. Rheumatoid involvement on the flexor aspect of the hand is common, but is easily overlooked because of difficulty in clinical examination. It is our experience that this localization of the disease process is extremely important and that surgical treatment should be given high priority. In this study a series of 235 operations on the flexor aspect of the hand performed on 139 patients are presented. The results are very gratifying as far as tenosynovectomy in the carpal tunnel and the pain is concerned. When performed on the fingers, however, varying degrees of postoperative flexion contractures developed in no less than 44%. It was found that this problem arose when the synovectomy was extended beyond the level of the middle flexion crease of the finger being caused by excessive postoperative scar formation in the region volar to the proximal interphalangeal joint between the tails of the superficial tendon. It is concluded that less extensive surgery should be aimed at in this particular area."}
{"id": "PMID:1085033", "title": "Ambulance transport of obstetric emergencies.", "content": "Ninety-eight patient records from obstetric flying squad calls were reviewed. The reasons for call-out and the effect of transport on maternal and foetal cardiovascular parameters were analysed. The main reason for calling the flying squad is now antepartum haemorrhage. Transporting the patient to hospital has little effect on the cardiovascular state.", "contents": "Ambulance transport of obstetric emergencies. Ninety-eight patient records from obstetric flying squad calls were reviewed. The reasons for call-out and the effect of transport on maternal and foetal cardiovascular parameters were analysed. The main reason for calling the flying squad is now antepartum haemorrhage. Transporting the patient to hospital has little effect on the cardiovascular state."}
{"id": "PMID:1085034", "title": "Binding of C-reactive protein to antigen-induced but not mitogen-induced T lymphoblasts.", "content": "The C-reactive protein (CRP), an acute phase reactant which binds selectively to T (thymus-derived) lymphocytes, was found to bind to lymphoblasts formed upon stimulation with antigens but not with mitogens. Binding of CRP thus serves as a marker for antigen-reactive (-reacted) as opposed to mitogen-reative (-reacted) T cells, suggesting that these represent separate subpopulations, and supports the developing concept that CRP play an important role in the regulation of responses critical to  inflammation, host defense, and tissue repair.", "contents": "Binding of C-reactive protein to antigen-induced but not mitogen-induced T lymphoblasts. The C-reactive protein (CRP), an acute phase reactant which binds selectively to T (thymus-derived) lymphocytes, was found to bind to lymphoblasts formed upon stimulation with antigens but not with mitogens. Binding of CRP thus serves as a marker for antigen-reactive (-reacted) as opposed to mitogen-reative (-reacted) T cells, suggesting that these represent separate subpopulations, and supports the developing concept that CRP play an important role in the regulation of responses critical to  inflammation, host defense, and tissue repair."}
{"id": "PMID:1085035", "title": "1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay.", "content": "A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients.", "contents": "1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay. A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients."}
{"id": "PMID:1085036", "title": "Gastric devascularization: an alternate approach to the surgical treatment of massive, diffuse hemorrhage from gastritis.", "content": "During a three-year period on the surgical services at the University of Texas in San Antonio, eight critically ill patients had various types of gastric devascularization to control erosive hemorrhagic gastritis. Among these patients were two women and one child. There were five chronic alcoholics, one aspirin abuser, one severely burned patient, and one patient with sepsis. Esophagogastroscopy was used in six patients but was diagnostic in only three. All of the patients were given intensive medical preoperative care, including an average of eight units of blood each. In four of the five known alcoholics, preoperative tests showed severely deranged clotting function. All of the patients received at least a four-point gastric vessel ligation. In addition, four had gastrotomies, three had vagotomies and pyloroplasties, and two had \"complete\" gastric vessel ligation. There was no significant rebleeding in five patients. Of the three patients with significant recurrent gastric hemorrhage, two were chronic alcoholics with poor clotting function, and neither was considered operable. One patient, the child, had massive rebleeding one week after gastric devascularization with vagotomy and pyloroplasty and required a subtotal gastrectomy. Of the four patients who ultimately died, three were chronic alcoholics and one had sepsis.", "contents": "Gastric devascularization: an alternate approach to the surgical treatment of massive, diffuse hemorrhage from gastritis. During a three-year period on the surgical services at the University of Texas in San Antonio, eight critically ill patients had various types of gastric devascularization to control erosive hemorrhagic gastritis. Among these patients were two women and one child. There were five chronic alcoholics, one aspirin abuser, one severely burned patient, and one patient with sepsis. Esophagogastroscopy was used in six patients but was diagnostic in only three. All of the patients were given intensive medical preoperative care, including an average of eight units of blood each. In four of the five known alcoholics, preoperative tests showed severely deranged clotting function. All of the patients received at least a four-point gastric vessel ligation. In addition, four had gastrotomies, three had vagotomies and pyloroplasties, and two had \"complete\" gastric vessel ligation. There was no significant rebleeding in five patients. Of the three patients with significant recurrent gastric hemorrhage, two were chronic alcoholics with poor clotting function, and neither was considered operable. One patient, the child, had massive rebleeding one week after gastric devascularization with vagotomy and pyloroplasty and required a subtotal gastrectomy. Of the four patients who ultimately died, three were chronic alcoholics and one had sepsis."}
{"id": "PMID:1085038", "title": "Cerebellopontine angle meningioma presenting as subarachnoid haemorrhage.", "content": "Two cases of cerebellopontine angle meningioma causing subarachnoid haemorrhage are described. Their clinical and pathological significance is discussed.", "contents": "Cerebellopontine angle meningioma presenting as subarachnoid haemorrhage. Two cases of cerebellopontine angle meningioma causing subarachnoid haemorrhage are described. Their clinical and pathological significance is discussed."}
{"id": "PMID:1085039", "title": "In vitro effect of thymosin on T cells from immunodepressed surgical patients.", "content": "In preparation for the use of bovine thymosin, a thymic hormone, as a specific T cell stimulator in immunodepressed patients, we studied its effect on E rosette formation of peripheral lymphocytes from patients with (1) advanced malignancies, (2) extensive burns, and (3) septicemia. E rosette formation in vitro with and without thymosin was evaluated in 52 patients with carcinoma of the breast (25) or lung (27) in relation to adjuvant therapy and/or surgery. The depression of E rosettes in cancer patients was most striking when adjuvant therapy, irradiation, and/or chemotherapy were used; in 20 patients this was elevated by incubation with thymosin. There was a delay in recovery of depressed E rosette levels after radical mastectomies in four patients, recovery being accelerated by thymosin. In ten burn patients (40 to 80 percent of body surface area, second and third degree burns), the depression in E rosette levels occurred in the first week and was most marked in 3 to 4 weeks. In eight patients this was elevated by thymosin in vitro. In four septic patients, all undergoing operation, serial studies suggested that recovery from sepsis was accompanied by spontaneous rise in E rosette levels. This process was accelerated by thymosin in vitro. This study as well as previous experiments with animals suggest that thymosin may influence depressed host resistance favorably by increasing T-cell-mediated immunity.", "contents": "In vitro effect of thymosin on T cells from immunodepressed surgical patients. In preparation for the use of bovine thymosin, a thymic hormone, as a specific T cell stimulator in immunodepressed patients, we studied its effect on E rosette formation of peripheral lymphocytes from patients with (1) advanced malignancies, (2) extensive burns, and (3) septicemia. E rosette formation in vitro with and without thymosin was evaluated in 52 patients with carcinoma of the breast (25) or lung (27) in relation to adjuvant therapy and/or surgery. The depression of E rosettes in cancer patients was most striking when adjuvant therapy, irradiation, and/or chemotherapy were used; in 20 patients this was elevated by incubation with thymosin. There was a delay in recovery of depressed E rosette levels after radical mastectomies in four patients, recovery being accelerated by thymosin. In ten burn patients (40 to 80 percent of body surface area, second and third degree burns), the depression in E rosette levels occurred in the first week and was most marked in 3 to 4 weeks. In eight patients this was elevated by thymosin in vitro. In four septic patients, all undergoing operation, serial studies suggested that recovery from sepsis was accompanied by spontaneous rise in E rosette levels. This process was accelerated by thymosin in vitro. This study as well as previous experiments with animals suggest that thymosin may influence depressed host resistance favorably by increasing T-cell-mediated immunity."}
{"id": "PMID:1085042", "title": "Free internal mammary artery for coronary bypass.", "content": "An improved technique of free internal mammary artery (IMA) to ascending aorta anastomosis for coronary bypass is described. A small patch vein with a suitable side branch or branches is interposed, connecting the proximal end of a detached IMA to the ascending aorta. A single or double free IMA alone or in combination with an additional saphenous vein graft was performed in 77 patients. A total of 125 free IMA grafts were done. The hospital mortality was 2-7% and late mortality 1-4%. Seventy-one of the 74 surviving patients had no angina and returned to a normal life. Postoperative coronary arteriography in 65 patients showed a patency rate of 96%. On year's experience with this technique is very encouraging. However, prolonged follow-up is necessary to determine if free IMA, as used by us, will increase the long-term patency rate.", "contents": "Free internal mammary artery for coronary bypass. An improved technique of free internal mammary artery (IMA) to ascending aorta anastomosis for coronary bypass is described. A small patch vein with a suitable side branch or branches is interposed, connecting the proximal end of a detached IMA to the ascending aorta. A single or double free IMA alone or in combination with an additional saphenous vein graft was performed in 77 patients. A total of 125 free IMA grafts were done. The hospital mortality was 2-7% and late mortality 1-4%. Seventy-one of the 74 surviving patients had no angina and returned to a normal life. Postoperative coronary arteriography in 65 patients showed a patency rate of 96%. On year's experience with this technique is very encouraging. However, prolonged follow-up is necessary to determine if free IMA, as used by us, will increase the long-term patency rate."}
{"id": "PMID:1085044", "title": "[Antimycotic antibiotics (author's transl)].", "content": "A number of antibiotics having an antimycotic action are reviewed. The physicochemical and pharmacotoxicological properties, antifungal range, mechanisms of action, dosage and preparations of griseofulvin, amphotericin-B, natamycin, nystatin and pecilocin are discussed.", "contents": "[Antimycotic antibiotics (author's transl)]. A number of antibiotics having an antimycotic action are reviewed. The physicochemical and pharmacotoxicological properties, antifungal range, mechanisms of action, dosage and preparations of griseofulvin, amphotericin-B, natamycin, nystatin and pecilocin are discussed."}
{"id": "PMID:1085045", "title": "Passive immunological enhancement of muscle allografts in rats.", "content": "The effect of the passive administration of antiserum obtained from recipients immunized with antigens from H-1 + non-H-1 incompatible donors on muscle allograft survival in rats was studied. In transplantation across the non-H-1 antigenic barrier (H-1 compatible but non-H-1 incompatible) a satisfactory degree of immunological enhancement was achieved. Recovery of contractile properties of muscle allografts after different immunological treatments of the recipients was compared.", "contents": "Passive immunological enhancement of muscle allografts in rats. The effect of the passive administration of antiserum obtained from recipients immunized with antigens from H-1 + non-H-1 incompatible donors on muscle allograft survival in rats was studied. In transplantation across the non-H-1 antigenic barrier (H-1 compatible but non-H-1 incompatible) a satisfactory degree of immunological enhancement was achieved. Recovery of contractile properties of muscle allografts after different immunological treatments of the recipients was compared."}
{"id": "PMID:1085048", "title": "[The effect of hypertonic solutions on contracture of a tonic muscle fiber].", "content": "Effect of glycerol, sucrose and NaC1 hypertonic solutions exerted on potassium contracture and resting membrane potential in isolated frog tonic muscle fibres were studied. Sucrose and NaC1 hypertonic solutions induce a stable depolarization and depress potassium contracture, while glycerol results in a transient depolarization followed by recovery of resting membrane potential and contracture reactions. The removal of 400 mM glycerol induces an insignificant depolarization and disappearance of contracture reactions, however, after the removal of sucrose of NaC1 hypertonicity both the parameters tend to restore. The isolated phasic and tonic fibres lose irreversibly potassium contracture reactions after the removal of 100 mM and 400 mM glycerol, respectively, the latter being more resistant to this procedure. Nevertheless it is concluded that the T-system is equally important for excitation--contraction coupling in tonic and in phasic fibres.", "contents": "[The effect of hypertonic solutions on contracture of a tonic muscle fiber]. Effect of glycerol, sucrose and NaC1 hypertonic solutions exerted on potassium contracture and resting membrane potential in isolated frog tonic muscle fibres were studied. Sucrose and NaC1 hypertonic solutions induce a stable depolarization and depress potassium contracture, while glycerol results in a transient depolarization followed by recovery of resting membrane potential and contracture reactions. The removal of 400 mM glycerol induces an insignificant depolarization and disappearance of contracture reactions, however, after the removal of sucrose of NaC1 hypertonicity both the parameters tend to restore. The isolated phasic and tonic fibres lose irreversibly potassium contracture reactions after the removal of 100 mM and 400 mM glycerol, respectively, the latter being more resistant to this procedure. Nevertheless it is concluded that the T-system is equally important for excitation--contraction coupling in tonic and in phasic fibres."}
{"id": "PMID:1085049", "title": "[Nuclear structures in the late oogenesis of Rana temporaria. III. Electron microscopic studies].", "content": "The ultrastructural organization of the vitellogenic oocyte nucleus (stage VI, according to Duryee, 1950) was studied in normal and in vitro hormone-stimulated maturing oocytes of Rana temporaria. At this stage, numerous nucleoli are gathered around the knot of highly contracted chromosomes (the karyosphere) thus making the karyosphere capsule. Light microscope observations reveal three zones in the capsule: a central fibrous zone separating the chromosomes from the nucleoli, a middle zone, consisting of numerous nucleoli and a distinct fibrous componen; in addition a fibrous zone on the capsule periphery is seen. The nucleoli are fibrillar, bear no proribosomal granules and do not synthesize RNA. This period is characterized by an intensive fragmentation and segregation of the nucleolar material. Numerous micronucleoli and nuclear bodies occur in the nucleus. The nucleoli are normally compound and irregular in shape to become spherical in hormone-stimulated maturing oocytes. In the central fibrous zone of the capsule, separating the chromosomes from the nucleoli, some peculiar abundant accumulations of annuli were detected lacking the membranes component. Annuli are linked with the fibrous material and are regularily packed making peculiar pseudomembranes (PMM). The chromatin is connected with PMM directly. In the middle zone of the capsule, accumulations of PMM are also seen, though less abundant and less regularly packed; along with annuli, membranous areas of various size and form are met in PMM. PMM are connected with the micronucleoli with filaments 20 nm thick. In the peripheral zone of the capsule, a variety of membranous structures is detected: intranuclear annuli lamellae, component of the capsule consists of different membranous and pseudomembranous (with annuli) structures. A question of the contribution of the chromatin material in the formation of the fibrous capsular component (PMM and membranous structures) is discussed.", "contents": "[Nuclear structures in the late oogenesis of Rana temporaria. III. Electron microscopic studies]. The ultrastructural organization of the vitellogenic oocyte nucleus (stage VI, according to Duryee, 1950) was studied in normal and in vitro hormone-stimulated maturing oocytes of Rana temporaria. At this stage, numerous nucleoli are gathered around the knot of highly contracted chromosomes (the karyosphere) thus making the karyosphere capsule. Light microscope observations reveal three zones in the capsule: a central fibrous zone separating the chromosomes from the nucleoli, a middle zone, consisting of numerous nucleoli and a distinct fibrous componen; in addition a fibrous zone on the capsule periphery is seen. The nucleoli are fibrillar, bear no proribosomal granules and do not synthesize RNA. This period is characterized by an intensive fragmentation and segregation of the nucleolar material. Numerous micronucleoli and nuclear bodies occur in the nucleus. The nucleoli are normally compound and irregular in shape to become spherical in hormone-stimulated maturing oocytes. In the central fibrous zone of the capsule, separating the chromosomes from the nucleoli, some peculiar abundant accumulations of annuli were detected lacking the membranes component. Annuli are linked with the fibrous material and are regularily packed making peculiar pseudomembranes (PMM). The chromatin is connected with PMM directly. In the middle zone of the capsule, accumulations of PMM are also seen, though less abundant and less regularly packed; along with annuli, membranous areas of various size and form are met in PMM. PMM are connected with the micronucleoli with filaments 20 nm thick. In the peripheral zone of the capsule, a variety of membranous structures is detected: intranuclear annuli lamellae, component of the capsule consists of different membranous and pseudomembranous (with annuli) structures. A question of the contribution of the chromatin material in the formation of the fibrous capsular component (PMM and membranous structures) is discussed."}
{"id": "PMID:1085050", "title": "Impact of BCG vaccination programmes in children and young adults on the tuberculosis problem.", "content": "There is general agreement that BCG vaccination with a potent strain, when given to previously uninfected subjects, is highly effective in preventing the development to tuberculosis among them. This \"direct\" effect may be measured in practice in terms of the proportion of cases prevented in the age-groups in which the vaccination has been made. It is also clamined that mass BCG vaccination-especially at school-leaving age-can be expected to yield benefits not only directly, but also indirectly, by breaking the chain of transmission and so preventing the development of tuberculosis in unvaccinated subjects. This \"indirect\" effect may be measured, by analogy, in terms of the reduction in the numbers of cases in the age groups in which no vaccinations have been performed (the older age groups, and also young children, if BCG vaccination is given, for instance, to school-leavers). The indirect effect will be observed in terms of changes in both smear-positive and smear-negative cases. However, because smear-positive cases are the principal sources of infection, it is more meaningful to measure the indirect effect in terms of the reduction in the numbers of smear-positive (infectious) cases. The present paper falls into three parts: I. A comparison of the trends in the incidence of infectious tuberculosis in Norway, Denmark and The Netherlands. The study confirms that the substantial direct effect of BCG vaccination on the total tuberculosis incidence, which was shown by Bjartveit and Waaler, also applies to the incidence of infectious tuberculosis. II. A comparison of the trends in the incidence of infectious tuberculosis in the three countries and in different age groups over 30, and in the incidence of all forms of tuberculosis in children, in whom BCG vaccination has not been used. These comparisons suggest that the indirect effect of BCG on infectious cases of tuberculosis in persons aged 30 years and over, and on all forms of tuberculosis in children, in whom BCG vaccination has not been used, is not readily detectable, and so may not be large. However, the trends in tuberculosis incidence measure the total effects of all the policies for control used in the three countries under study. The comparisons between the three countries can therefore not isolate the \"pure\" direct and indirect effects of BCG from the effects of other differences between the control policies. For this reason a theoretical study of the problem has been made. III. A theoretical examination of the maximum likely indirect effect of a mass BCG vaccination policy in diminishing the prevalence of sources of infection in the population. This shows that the effect of BCG in preventing smear-positive cases in developing countries (if a constant risk of infection of about 3 per cent is presumed), is between 0.3 per cent and 2.0 per cent per year. This effect depends mainly upon the efficacy of BCG vaccine, the population covered and the duration of protection from BCG vaccination...", "contents": "Impact of BCG vaccination programmes in children and young adults on the tuberculosis problem. There is general agreement that BCG vaccination with a potent strain, when given to previously uninfected subjects, is highly effective in preventing the development to tuberculosis among them. This \"direct\" effect may be measured in practice in terms of the proportion of cases prevented in the age-groups in which the vaccination has been made. It is also clamined that mass BCG vaccination-especially at school-leaving age-can be expected to yield benefits not only directly, but also indirectly, by breaking the chain of transmission and so preventing the development of tuberculosis in unvaccinated subjects. This \"indirect\" effect may be measured, by analogy, in terms of the reduction in the numbers of cases in the age groups in which no vaccinations have been performed (the older age groups, and also young children, if BCG vaccination is given, for instance, to school-leavers). The indirect effect will be observed in terms of changes in both smear-positive and smear-negative cases. However, because smear-positive cases are the principal sources of infection, it is more meaningful to measure the indirect effect in terms of the reduction in the numbers of smear-positive (infectious) cases. The present paper falls into three parts: I. A comparison of the trends in the incidence of infectious tuberculosis in Norway, Denmark and The Netherlands. The study confirms that the substantial direct effect of BCG vaccination on the total tuberculosis incidence, which was shown by Bjartveit and Waaler, also applies to the incidence of infectious tuberculosis. II. A comparison of the trends in the incidence of infectious tuberculosis in the three countries and in different age groups over 30, and in the incidence of all forms of tuberculosis in children, in whom BCG vaccination has not been used. These comparisons suggest that the indirect effect of BCG on infectious cases of tuberculosis in persons aged 30 years and over, and on all forms of tuberculosis in children, in whom BCG vaccination has not been used, is not readily detectable, and so may not be large. However, the trends in tuberculosis incidence measure the total effects of all the policies for control used in the three countries under study. The comparisons between the three countries can therefore not isolate the \"pure\" direct and indirect effects of BCG from the effects of other differences between the control policies. For this reason a theoretical study of the problem has been made. III. A theoretical examination of the maximum likely indirect effect of a mass BCG vaccination policy in diminishing the prevalence of sources of infection in the population. This shows that the effect of BCG in preventing smear-positive cases in developing countries (if a constant risk of infection of about 3 per cent is presumed), is between 0.3 per cent and 2.0 per cent per year. This effect depends mainly upon the efficacy of BCG vaccine, the population covered and the duration of protection from BCG vaccination..."}
{"id": "PMID:1085054", "title": "The antigenicity of venous allografts.", "content": "Segments of rat inferior vena cava modified by proteolytic digestion and dialdehyde starch tanning were found to retain their ability to sensitize allogeneic recipients both in the intradermal and intra-aortic positions. Furthermore, these grafts had a very high aneurysm formation (100%) and aneurysm rupture rate (63%), even when used as isografts, suggesting that the modification procedure so damages the structural integrity of the grafts that use in clinical situations may have disastrous consequences. Clinical trials must be undertaken with great caution and a mild, brief course of immunosuppression may be indicated when modified venous allografts are contemplated in potential organ transplant recipients. Furthermore, prosthetic mesh may be required for support whenever modified allogeneic veins are used for arterial substitutes. In the absence of autologous tissue, the bovine xenograft is probably the material of choice for reconstruction or bypass of small vessels.", "contents": "The antigenicity of venous allografts. Segments of rat inferior vena cava modified by proteolytic digestion and dialdehyde starch tanning were found to retain their ability to sensitize allogeneic recipients both in the intradermal and intra-aortic positions. Furthermore, these grafts had a very high aneurysm formation (100%) and aneurysm rupture rate (63%), even when used as isografts, suggesting that the modification procedure so damages the structural integrity of the grafts that use in clinical situations may have disastrous consequences. Clinical trials must be undertaken with great caution and a mild, brief course of immunosuppression may be indicated when modified venous allografts are contemplated in potential organ transplant recipients. Furthermore, prosthetic mesh may be required for support whenever modified allogeneic veins are used for arterial substitutes. In the absence of autologous tissue, the bovine xenograft is probably the material of choice for reconstruction or bypass of small vessels."}
{"id": "PMID:1085057", "title": "[Acute thrombophlebitis and phlebothrombosis of the portal vein in the postoperative period].", "content": "The symptomatology of acute thrombophlebitis and phlebothrombosis of the portal vein is described. The clinical difference between these two grave and rarely observed pathological processes in emphasized. Two observations are reported.", "contents": "[Acute thrombophlebitis and phlebothrombosis of the portal vein in the postoperative period]. The symptomatology of acute thrombophlebitis and phlebothrombosis of the portal vein is described. The clinical difference between these two grave and rarely observed pathological processes in emphasized. Two observations are reported."}
{"id": "PMID:1085058", "title": "[Use of low-frequency currents in the treatment of severe intestinal paralysis].", "content": "Low-frequency impulse currents (Bernar current) are suggested for treatment of paralytic intestinal obstruction. The method was used in 108 patients, in whom the cause of paresis was as follows: acute purulent peritonitis, pancreatitis, operations on the abdominal aorta and its branches. In 102 patients the stimulation proved to be effective, in 6 cases of progressive purulent peritonitis no effect was gained. In a number of patients the procedure had to be repeated during 2--3 days. Generally, diadynamic currents were employed in a complex with other measures: syphon enema, paranephral blockade, cholinergics, etc. A grave cardiac pathology and oncological diseases are considered as contraindications.", "contents": "[Use of low-frequency currents in the treatment of severe intestinal paralysis]. Low-frequency impulse currents (Bernar current) are suggested for treatment of paralytic intestinal obstruction. The method was used in 108 patients, in whom the cause of paresis was as follows: acute purulent peritonitis, pancreatitis, operations on the abdominal aorta and its branches. In 102 patients the stimulation proved to be effective, in 6 cases of progressive purulent peritonitis no effect was gained. In a number of patients the procedure had to be repeated during 2--3 days. Generally, diadynamic currents were employed in a complex with other measures: syphon enema, paranephral blockade, cholinergics, etc. A grave cardiac pathology and oncological diseases are considered as contraindications."}
{"id": "PMID:1085059", "title": "[Hemorrhage after endoscopic polypectomy of the stomach].", "content": "46 endoscopic electroexcisions of gastric polyps were performed in one case this procedure was complicated with bleeding, that would not be arrested via an endofibroscope. This operative procedure is described, and also some considerations concerning endoscopic electroexcision of gastric polyps are given.", "contents": "[Hemorrhage after endoscopic polypectomy of the stomach]. 46 endoscopic electroexcisions of gastric polyps were performed in one case this procedure was complicated with bleeding, that would not be arrested via an endofibroscope. This operative procedure is described, and also some considerations concerning endoscopic electroexcision of gastric polyps are given."}
{"id": "PMID:1085060", "title": "[Arrest of gastroduodenal hemorrhage under the control of a fibroscope].", "content": "The method of electrocoagulation of gastroduodenal bleeding sources by means of a fibroscope allows a reliable and easy arresting of bleeding even in emaciated and exsanguinated patients, This method seems to be especially valuable in cases, when the source of bleeding is diagnosed only under endoscopy. In such cases a high diagnostic precision is associated with therapeutic manipulations, and in some cases it gives the chance to avoid surgery at the height of bleeding. An electrode used by the author is simple in design and readily available. Visual hemostasis, the formation of a solid crust in the hemorrhagic region and disappearance of clinical manifestations of gastro-duodenal hemorrhage may serve as a criterion of the effective arrest of bleeding.", "contents": "[Arrest of gastroduodenal hemorrhage under the control of a fibroscope]. The method of electrocoagulation of gastroduodenal bleeding sources by means of a fibroscope allows a reliable and easy arresting of bleeding even in emaciated and exsanguinated patients, This method seems to be especially valuable in cases, when the source of bleeding is diagnosed only under endoscopy. In such cases a high diagnostic precision is associated with therapeutic manipulations, and in some cases it gives the chance to avoid surgery at the height of bleeding. An electrode used by the author is simple in design and readily available. Visual hemostasis, the formation of a solid crust in the hemorrhagic region and disappearance of clinical manifestations of gastro-duodenal hemorrhage may serve as a criterion of the effective arrest of bleeding."}
{"id": "PMID:1085061", "title": "[Evaluation of the hazard of surgical intervention at the height of gastroduodenal hemorrhage].", "content": "Based on the experimental data obtained, it was concluded that the danger of surgery at the height of the primary hemorrhage, although massive it may be, is somewhat exaggerated by surgical specialists. Recurrent in a short period of time hemorrhages are believed to be more inherent in danger than an operation in primary bleeding. The results of the experiments are supported by observations over 70 patients, operated upon for ulcerous bleeding, and in 11 patients who were subjected to surgery for profuse hemorrhage from cancerous gastric ulcers.", "contents": "[Evaluation of the hazard of surgical intervention at the height of gastroduodenal hemorrhage]. Based on the experimental data obtained, it was concluded that the danger of surgery at the height of the primary hemorrhage, although massive it may be, is somewhat exaggerated by surgical specialists. Recurrent in a short period of time hemorrhages are believed to be more inherent in danger than an operation in primary bleeding. The results of the experiments are supported by observations over 70 patients, operated upon for ulcerous bleeding, and in 11 patients who were subjected to surgery for profuse hemorrhage from cancerous gastric ulcers."}
{"id": "PMID:1085067", "title": "Differentiation of human leukemic from normal lymphocytes by Limulus serum agglutination.", "content": "Human peripheral chronic lymphocytic leukemic lymphocytes and a line (B411-4) of cultured human B cells are agglutinated by Limulus serum to a significantly higher titer and score than peripheral normal human lymphocytes or a line (MOLT-4-F) of cultured human T cells.", "contents": "Differentiation of human leukemic from normal lymphocytes by Limulus serum agglutination. Human peripheral chronic lymphocytic leukemic lymphocytes and a line (B411-4) of cultured human B cells are agglutinated by Limulus serum to a significantly higher titer and score than peripheral normal human lymphocytes or a line (MOLT-4-F) of cultured human T cells."}
{"id": "PMID:1085069", "title": "Risk factors and coronary artery obstruction in male and female patients with aortocoronary bypass operation.", "content": "In order to learn about some possible reasons for the low proportion of women among the patients with aortocoronary bypass operation (12-20%) we have compared the prevalence of hypertension, hyperlipidemia, history of smoking and chest pain in 110 women and 813 men with this surgical intervention. In spite of the similar or even higher prevalence of abnormal risk factors, the women patients showed less extensive coronary artery impairment than the male patients indicating their higher tolerance of the risk factors and lesser need for the bypass operation.", "contents": "Risk factors and coronary artery obstruction in male and female patients with aortocoronary bypass operation. In order to learn about some possible reasons for the low proportion of women among the patients with aortocoronary bypass operation (12-20%) we have compared the prevalence of hypertension, hyperlipidemia, history of smoking and chest pain in 110 women and 813 men with this surgical intervention. In spite of the similar or even higher prevalence of abnormal risk factors, the women patients showed less extensive coronary artery impairment than the male patients indicating their higher tolerance of the risk factors and lesser need for the bypass operation."}
{"id": "PMID:1085080", "title": "[Structural organization of nephrons in the kideny and proximal reabsorption in representatives of different vertebrate classes (according to microdissection and micropuncture studies)].", "content": "By microdissection method, single nephrons have been isolated from the river lamprey Lampetra fluviatilis, teleost fish Myoxocephalus scorpius (White sea), frog Rana temporaria, lizard Agama caucasica, hen Gallus domesticus and albino rat Rattus norvegicus (Wistar strain). Tubular reabsorptive capacity was measured in kidneys of lampreys and rats by the split oil droplet method. In the animals studied, each of the nephrons consists of the glomerulus, proximal, thin and distal segments. Relative length of the proximal segment does not depend on phylogenetic position of a species. Transtubular isotonic water reabsorption from the proximal tubule in rats is significantly higher than in lampreys. The level of proximal reabsorption is not related to the length of the proximal tubule in the Verterbrates studied but depends on the intensity of tubular transport. The structure which is similar to Henle's loop is present already in Cyclostomes [12]. Further stage of the progressive development of the uriniferous tubules is presented by uneven localization of nephron populations within the kideney in Reptiles. These data suggest that the effective system of osmotic concentration appeared in evolution not as the result of Henle's loop formation, but on the basis of the development of the medullar substance formed mainly by intercortical and juxtamedullar nephron populations.", "contents": "[Structural organization of nephrons in the kideny and proximal reabsorption in representatives of different vertebrate classes (according to microdissection and micropuncture studies)]. By microdissection method, single nephrons have been isolated from the river lamprey Lampetra fluviatilis, teleost fish Myoxocephalus scorpius (White sea), frog Rana temporaria, lizard Agama caucasica, hen Gallus domesticus and albino rat Rattus norvegicus (Wistar strain). Tubular reabsorptive capacity was measured in kidneys of lampreys and rats by the split oil droplet method. In the animals studied, each of the nephrons consists of the glomerulus, proximal, thin and distal segments. Relative length of the proximal segment does not depend on phylogenetic position of a species. Transtubular isotonic water reabsorption from the proximal tubule in rats is significantly higher than in lampreys. The level of proximal reabsorption is not related to the length of the proximal tubule in the Verterbrates studied but depends on the intensity of tubular transport. The structure which is similar to Henle's loop is present already in Cyclostomes [12]. Further stage of the progressive development of the uriniferous tubules is presented by uneven localization of nephron populations within the kideney in Reptiles. These data suggest that the effective system of osmotic concentration appeared in evolution not as the result of Henle's loop formation, but on the basis of the development of the medullar substance formed mainly by intercortical and juxtamedullar nephron populations."}
{"id": "PMID:1085081", "title": "[A direct microfluorimetric study of organic acid passage through the proximal tubule cells of the surviving Rana temporaria frog kidney].", "content": "Accumulation of acid fluorochrome in proximal tubuli in the frog surviving kidney has been investigated. Mean concentration of fluorochrome in the tubuli is linearly related to the duration of incubation. Changes in the mean rate of transport of fluorochrome into the tubuli induced by changes in its external concentration are satisfactorily described by Michaelis-Menten equation, Michaelis constant being equal to 2.1-10(-4). Some equations are suggested which describe the kinetics of organic acid transport across basal and apical membranes of cells.", "contents": "[A direct microfluorimetric study of organic acid passage through the proximal tubule cells of the surviving Rana temporaria frog kidney]. Accumulation of acid fluorochrome in proximal tubuli in the frog surviving kidney has been investigated. Mean concentration of fluorochrome in the tubuli is linearly related to the duration of incubation. Changes in the mean rate of transport of fluorochrome into the tubuli induced by changes in its external concentration are satisfactorily described by Michaelis-Menten equation, Michaelis constant being equal to 2.1-10(-4). Some equations are suggested which describe the kinetics of organic acid transport across basal and apical membranes of cells."}
{"id": "PMID:1085082", "title": "[Distribution of three types of muscle fiber in the skeletal muscles of Rana temporaria frogs (according to histochemical findings)].", "content": "On 15 skeletal muscles with different functional profile (flexors and extensors, trunk muscles and muscles of the head) and different embryonic origin (somatic and visceral), it has been shown that muscles of R. temporaria are mixed. Muscle fibers are divided into three groups which differ in the content of lipids, the activity of succinic dehydrogenase and localization of myofibrils on a cross section. On the basis of the data obtained and those in literature, these fiber groups are qualified as phasic, tonic and transitional (slow phasic) ones. Both phasic and tonic fiber groups are uniform, whereas transitional fibers form a broad specturm. Most of the muscles studied contain all the three types of fibers. In muscles of the extremities (especially in flexors), tonic fibers together with transitional ones usually form more or less compact bundles. The highest content of tonic and transitional fibers was found in muscles which do not directly participate in locomotion. If muscles contain only two types of fibers (phasic and transitional ones), these fibers form a mosaic structure.", "contents": "[Distribution of three types of muscle fiber in the skeletal muscles of Rana temporaria frogs (according to histochemical findings)]. On 15 skeletal muscles with different functional profile (flexors and extensors, trunk muscles and muscles of the head) and different embryonic origin (somatic and visceral), it has been shown that muscles of R. temporaria are mixed. Muscle fibers are divided into three groups which differ in the content of lipids, the activity of succinic dehydrogenase and localization of myofibrils on a cross section. On the basis of the data obtained and those in literature, these fiber groups are qualified as phasic, tonic and transitional (slow phasic) ones. Both phasic and tonic fiber groups are uniform, whereas transitional fibers form a broad specturm. Most of the muscles studied contain all the three types of fibers. In muscles of the extremities (especially in flexors), tonic fibers together with transitional ones usually form more or less compact bundles. The highest content of tonic and transitional fibers was found in muscles which do not directly participate in locomotion. If muscles contain only two types of fibers (phasic and transitional ones), these fibers form a mosaic structure."}
{"id": "PMID:1085084", "title": "[Resistance to heating and trypsin of glutamate dehydrogenase from liver mitochondria of frogs differing in thermotaxis].", "content": "The resistances to heat and trypsin hydrolysis served as indirect indices of conformational flexibility of glutamate dehydrogenase molecules. No difference in heat resistance was found between crystalline eletrophoretically homogeneous preparations of glutamate dehydrogenase from liver mitochondria of two species of frogs, the more southern Rana ridibunda and the more northern R. temporaria. However, glutamate dehydrogenase from R. ridibunda is digested by trypsin at a lower rate than that from R. temporaria, which may be explained by its lower conformational flexibility. Therefore positive correlation between conformational flexibility of glutamate dehydrogenase and mean ambient temperature of the species studied is revealed only with respect to resistance to proteolysis.", "contents": "[Resistance to heating and trypsin of glutamate dehydrogenase from liver mitochondria of frogs differing in thermotaxis]. The resistances to heat and trypsin hydrolysis served as indirect indices of conformational flexibility of glutamate dehydrogenase molecules. No difference in heat resistance was found between crystalline eletrophoretically homogeneous preparations of glutamate dehydrogenase from liver mitochondria of two species of frogs, the more southern Rana ridibunda and the more northern R. temporaria. However, glutamate dehydrogenase from R. ridibunda is digested by trypsin at a lower rate than that from R. temporaria, which may be explained by its lower conformational flexibility. Therefore positive correlation between conformational flexibility of glutamate dehydrogenase and mean ambient temperature of the species studied is revealed only with respect to resistance to proteolysis."}
{"id": "PMID:1085089", "title": "[Gastrontestinal hemorrhage following thoracic surgery].", "content": "The authors discuss eight cases who suffered hemorrhaging stress ulcers out of 200 cases on whom Thoracotomies were performed. Presented is the common factor of the onset of this complication, it's diagnosis, and therapy. Listed below are the diagnoses and operative procedures used on these eight patients. Cysta Aerea Permagna Lobi Inf. Pulm. Dexter/operation: Mytomis Longitudinalis Ooesophagi. Caverna Bronchiectatica Permagna Lobi Inferior Pulmo Dexter/operation: Lobestomia Typica. Echinococcus Heaptis Complicatus, Empyema Pleurae Dexter/opetation: Decorticatio. Haemathorax Spontaneous Lobus Sinister/operation: Decorticatio Pleurae Sinister. Echi Comp. Cupolae Hepatis Permagnus/operation: Thoracotomia Phrenotomia, evacuatio, Triplex Drainage. Bronchiectasiae Lobi Medius et Inferior Pulmo Dexter/operation: Biblobectomia Typica. Carcinoma Bronchi Lobi Inferior Pulmo Dexter/operation: Lobectomia Typica. Gastric problems had troubled four of these eight patients in their past history. Bleeding in three patients occurred three days postoperatively, and in the remaining five, thirty days following their operation. Six patients had to be treated conservatively because of serious contraindications to reoperation. Four of them expired. Autopsy revealed: Pyothorax, Dehiscention Bronchi, Empyema, and Gastritis Errosiva with multiulcerations, hemoragia, and dilatation of the right heart. Two patients with recent stress ulcers were reoperated on, and were cured. The authors estimate that the occurrence of hemorrhaging stress ulcer following thoracic surgery are basically due to Hypoxia. The chain of events whic brought about the stress ulcer, however, began even before the operation, continued throughout the operation, and appeared postoperatively due to postoperative complications. The authors point out that these complications can be foreseen (early and late), but firstly, an attempt should be made to treat the patient with conservative therapy. Inasmuch as the hemorrhaging does not respond to conservative measures, conservative surgery is indicated; Suture and Vagotomy, and eventually pyloroplasty.", "contents": "[Gastrontestinal hemorrhage following thoracic surgery]. The authors discuss eight cases who suffered hemorrhaging stress ulcers out of 200 cases on whom Thoracotomies were performed. Presented is the common factor of the onset of this complication, it's diagnosis, and therapy. Listed below are the diagnoses and operative procedures used on these eight patients. Cysta Aerea Permagna Lobi Inf. Pulm. Dexter/operation: Mytomis Longitudinalis Ooesophagi. Caverna Bronchiectatica Permagna Lobi Inferior Pulmo Dexter/operation: Lobestomia Typica. Echinococcus Heaptis Complicatus, Empyema Pleurae Dexter/opetation: Decorticatio. Haemathorax Spontaneous Lobus Sinister/operation: Decorticatio Pleurae Sinister. Echi Comp. Cupolae Hepatis Permagnus/operation: Thoracotomia Phrenotomia, evacuatio, Triplex Drainage. Bronchiectasiae Lobi Medius et Inferior Pulmo Dexter/operation: Biblobectomia Typica. Carcinoma Bronchi Lobi Inferior Pulmo Dexter/operation: Lobectomia Typica. Gastric problems had troubled four of these eight patients in their past history. Bleeding in three patients occurred three days postoperatively, and in the remaining five, thirty days following their operation. Six patients had to be treated conservatively because of serious contraindications to reoperation. Four of them expired. Autopsy revealed: Pyothorax, Dehiscention Bronchi, Empyema, and Gastritis Errosiva with multiulcerations, hemoragia, and dilatation of the right heart. Two patients with recent stress ulcers were reoperated on, and were cured. The authors estimate that the occurrence of hemorrhaging stress ulcer following thoracic surgery are basically due to Hypoxia. The chain of events whic brought about the stress ulcer, however, began even before the operation, continued throughout the operation, and appeared postoperatively due to postoperative complications. The authors point out that these complications can be foreseen (early and late), but firstly, an attempt should be made to treat the patient with conservative therapy. Inasmuch as the hemorrhaging does not respond to conservative measures, conservative surgery is indicated; Suture and Vagotomy, and eventually pyloroplasty."}
{"id": "PMID:1085092", "title": "[Anuria caused by mercury poisoning (apropos of 15 cases)].", "content": "15 cases of acute renal failure following ingestion of bichloride of mercury were studied at Pahlavi medical center, university of Teheran, between 1962 and 1972. All the patients admitted suicide attempts. All had oliguria and uremia except one, in whom oliguria was moderate without azotemia. There were four deaths among the 15 patients, three of them with gastrointestinal hemorrhage; secondery infection and the prolonged acute uremia probably were the cause of death in another one. Early administration of B. A. L. and early frequent hemodialysis helps to support the patient through the period of oliguria and possibly to remove the mercury B.A.L. Complex. Although the mortality of mercury intoxication has decreased, gastrointestinal hemorrhages and secondery infections make its prognosis still unfavorable.", "contents": "[Anuria caused by mercury poisoning (apropos of 15 cases)]. 15 cases of acute renal failure following ingestion of bichloride of mercury were studied at Pahlavi medical center, university of Teheran, between 1962 and 1972. All the patients admitted suicide attempts. All had oliguria and uremia except one, in whom oliguria was moderate without azotemia. There were four deaths among the 15 patients, three of them with gastrointestinal hemorrhage; secondery infection and the prolonged acute uremia probably were the cause of death in another one. Early administration of B. A. L. and early frequent hemodialysis helps to support the patient through the period of oliguria and possibly to remove the mercury B.A.L. Complex. Although the mortality of mercury intoxication has decreased, gastrointestinal hemorrhages and secondery infections make its prognosis still unfavorable."}
{"id": "PMID:1085083", "title": "[Circadian cycles of wakefulness and rest in Rana temporaria frogs].", "content": "In the diurnal activity of the frog R. temporaria, rest and awakefulness stages were investigated. Resting stage was differentiated into daily and nocturnal phases. During daily rest, plastic tone in neck muscles was registered. During this phase, the head might be subjected to unnatural position which is maintained for a certain period. The heart rate was minimal, respiration could not be recorded. During nocturnal rest, in contrast to daily one, the animal changed its positon when touched at the head and kept itself in this positon. Cardiac contractions exhibited evident rhythmicity. Sleep episodes were rare and occasional; they were accompanied by eye closing and relaxation of the body.", "contents": "[Circadian cycles of wakefulness and rest in Rana temporaria frogs]. In the diurnal activity of the frog R. temporaria, rest and awakefulness stages were investigated. Resting stage was differentiated into daily and nocturnal phases. During daily rest, plastic tone in neck muscles was registered. During this phase, the head might be subjected to unnatural position which is maintained for a certain period. The heart rate was minimal, respiration could not be recorded. During nocturnal rest, in contrast to daily one, the animal changed its positon when touched at the head and kept itself in this positon. Cardiac contractions exhibited evident rhythmicity. Sleep episodes were rare and occasional; they were accompanied by eye closing and relaxation of the body."}
{"id": "PMID:1085093", "title": "Ventriculography with methylglucamine iocarmate (Dimer-X). Experimental and clinical study.", "content": "In an attempt to study the use and value in ventriculography of meglumine iocarmate (Dimer-X), previously accepted as a water soluble contrast medium safe for myelography, we have conducted animal experiments to compare Dimer-X with megulumine iothalamate (Conray 60) and meglumine diatrizoate (Angiografin). We also made clinical studies of Dimer-X. In  the animal experiments, 1.5 ml and 2.0 ml of Dimer-X were injected into the ventricles of 7 dogs. We noted the apparent superiority of Dimer-X over Conray 60 and Angiografin as far as side effects were concerned, but there were no particular differences in the intensities of the ventriculograms obtained. Morphological studies of the ventricles and histological examinations of the ventricular walls 1 month after intraventricular injections of Dimer-X showed no abnormalities. In the clinical studies, ventriculography with 1-5 ml Dimer-X, performed on 17 cases, aged 8 months to 62 years, with diseases of the central nervous system, produced ventriculograms of good diagnostic value with no side effects, such as convulsions, apart from mild headache or vomiting in 4 instances.", "contents": "Ventriculography with methylglucamine iocarmate (Dimer-X). Experimental and clinical study. In an attempt to study the use and value in ventriculography of meglumine iocarmate (Dimer-X), previously accepted as a water soluble contrast medium safe for myelography, we have conducted animal experiments to compare Dimer-X with megulumine iothalamate (Conray 60) and meglumine diatrizoate (Angiografin). We also made clinical studies of Dimer-X. In  the animal experiments, 1.5 ml and 2.0 ml of Dimer-X were injected into the ventricles of 7 dogs. We noted the apparent superiority of Dimer-X over Conray 60 and Angiografin as far as side effects were concerned, but there were no particular differences in the intensities of the ventriculograms obtained. Morphological studies of the ventricles and histological examinations of the ventricular walls 1 month after intraventricular injections of Dimer-X showed no abnormalities. In the clinical studies, ventriculography with 1-5 ml Dimer-X, performed on 17 cases, aged 8 months to 62 years, with diseases of the central nervous system, produced ventriculograms of good diagnostic value with no side effects, such as convulsions, apart from mild headache or vomiting in 4 instances."}
{"id": "PMID:1085090", "title": "Cerebellopontine angle tumours, other than acoustic neuromas. A report on 34 cases. A presentation of 7 bilateral acoustic neuromas.", "content": "The symptoms, preoperative diagnostic results and histological findings in 34 cerebellopontine angle tumour cases, other than acoustic neuromas, are presented. The meningeomas dominate by far this group of tumours. The symptomatology is much less uniform than that of the acoustic neuromas, where the VIII nerve is dominant. The facial nerve is less involved, whereas the other cranial nerves give symptoms more frequently. One remarkable sign is the presence of severe trigeminal neuralgia, which we have not encountered in acoustic neuromas. Only 35% of the 'non-neuroma' patients had elevated spinal fluid protein, compared with 100% in our acoustic neuroma cases. Furthermore, 7 patients with bilateral acoustic neuromas are presented. The connection with von Recklinghausens disease and multiple meningeomas is discussed.", "contents": "Cerebellopontine angle tumours, other than acoustic neuromas. A report on 34 cases. A presentation of 7 bilateral acoustic neuromas. The symptoms, preoperative diagnostic results and histological findings in 34 cerebellopontine angle tumour cases, other than acoustic neuromas, are presented. The meningeomas dominate by far this group of tumours. The symptomatology is much less uniform than that of the acoustic neuromas, where the VIII nerve is dominant. The facial nerve is less involved, whereas the other cranial nerves give symptoms more frequently. One remarkable sign is the presence of severe trigeminal neuralgia, which we have not encountered in acoustic neuromas. Only 35% of the 'non-neuroma' patients had elevated spinal fluid protein, compared with 100% in our acoustic neuroma cases. Furthermore, 7 patients with bilateral acoustic neuromas are presented. The connection with von Recklinghausens disease and multiple meningeomas is discussed."}
{"id": "PMID:1085094", "title": "Some cellular aspects of chronic inflammation in joint disease.", "content": "We have examined the nature of some mononuclear cells from inflamed synovial membranes of patients with rheumatoid arthritis. It was found that cells which remained in the supernatant medium after overnight culture of trypsinized tissue contained a variable number of lymphocytes which were shown to be T cells by rosetting and mitogen response. This suggests a source of T cell lymphokines with an effect on macrophages and thus a role in the maintenance of inflammation. Another role for mononuclear cells is suggest by the cytotoxicity of blood mononuclear cells directed against cultured synovial cells. This was found to occur in an autologous system using fibroblasts from rheumatoid synovium, but was not specific for rheumatoid arthritis. Stimulatory factors from rheumatoid joint effusion macrophages for blood lymphocytes were sought, but although blast transformation occurred, the results were equivocal. In this communication we set out to examine the nature of lymphoid cells in the synovial membrane and the role which they may play in the pathogenesis of chronic inflammation. We also briefly consider cell-mediated mechanisms of tissue injury. Since an active role of lymphoid cells pre-supposes the presence of an agent or agents which serve to stimulate them, we also report some recent attempts to find evidence for this. One of the striking histological features of the inflamed synovial tissue in rheumatoid arthritis, but also in some other forms of chronic arthritis, is the presence of lymphocytes and plasma cells. Immunofluorescent studies and organ culture studies have shown the presence and synthesis of immunoglobulins, predominantly the domain of plasma cells. The production of immunoglobulins, formation of complexes and activation of complement is a major factor in pathogenesis, but lymphocytes may also have a direct role through the production of lymphokines. Until recently these substances have been attributed to T lymphocytes, but Rocklin et al. (1) have recently shown that B cells may also be involved in certain experimental circumstances. The availability of synovectomy specimens from patients with rheumatoid arthritis has enabled us to examine the nature of lymphoid cells from synovial membranes. (This part of the work is reported fully elsewhere.", "contents": "Some cellular aspects of chronic inflammation in joint disease. We have examined the nature of some mononuclear cells from inflamed synovial membranes of patients with rheumatoid arthritis. It was found that cells which remained in the supernatant medium after overnight culture of trypsinized tissue contained a variable number of lymphocytes which were shown to be T cells by rosetting and mitogen response. This suggests a source of T cell lymphokines with an effect on macrophages and thus a role in the maintenance of inflammation. Another role for mononuclear cells is suggest by the cytotoxicity of blood mononuclear cells directed against cultured synovial cells. This was found to occur in an autologous system using fibroblasts from rheumatoid synovium, but was not specific for rheumatoid arthritis. Stimulatory factors from rheumatoid joint effusion macrophages for blood lymphocytes were sought, but although blast transformation occurred, the results were equivocal. In this communication we set out to examine the nature of lymphoid cells in the synovial membrane and the role which they may play in the pathogenesis of chronic inflammation. We also briefly consider cell-mediated mechanisms of tissue injury. Since an active role of lymphoid cells pre-supposes the presence of an agent or agents which serve to stimulate them, we also report some recent attempts to find evidence for this. One of the striking histological features of the inflamed synovial tissue in rheumatoid arthritis, but also in some other forms of chronic arthritis, is the presence of lymphocytes and plasma cells. Immunofluorescent studies and organ culture studies have shown the presence and synthesis of immunoglobulins, predominantly the domain of plasma cells. The production of immunoglobulins, formation of complexes and activation of complement is a major factor in pathogenesis, but lymphocytes may also have a direct role through the production of lymphokines. Until recently these substances have been attributed to T lymphocytes, but Rocklin et al. (1) have recently shown that B cells may also be involved in certain experimental circumstances. The availability of synovectomy specimens from patients with rheumatoid arthritis has enabled us to examine the nature of lymphoid cells from synovial membranes. (This part of the work is reported fully elsewhere."}
{"id": "PMID:1085095", "title": "Adjuvant-induced arthritis in four inbred strains of rats. An in vitro study of peripheral T and B lymphocytes.", "content": "The lymphoblastic response (LTT) to non-specific mitogens (PHA, PWM and ConA) of peripheral lymphocytes was investigated at days 0, 7, 14, 21 and 28 after adjuvant injection in four strains of inbred rats: Wistar (WAG), Long Evans (LE), Lewis (LEW) and Brown Norway (BN). LTT was assessed by using 18 hours H3 TdR incorporation in 5 days cultures of whole blood (micromethod). The statistical treatment of data, using principal components multifactorial analysis and analysis of variance showed a striking difference between strains. In control animals the responses to PHA and PWM were correlated and were higher in LE and WAG than in LEW and BN (BN=LEW less than LE=WAG). The response to ConA was independent of that to the other mitogens. It was generally low, but significantly higher in LEW and BN than in WAG and LE. In adjuvant-injected animals the responses to PHA and PWM were still correlated, but modified compared to control: in LE and LEW, but not in WAG and BN, a marked decrease of the response was found, reaching a minimum value within days 7 and 14. In the same time the response to ConA increased in the four strains, later in LE than in the others. However the intensity of the ConA response varied from one strain to another: it was constantly low in LE and WAG compared to LEW and BN. So the most striking modification of LTT were observed in LE and LEW, which both developed the most severe arthritis. However these different behaviours after adjuvant injection were not explained by the initial level of LTT to the different mitogens. These data suggest that the development of intense arthritis is associated with the proliferation and the release into the blood stream of a lymphocyte subpopulation, which exhibits a low response to PHA and PWM and a high response to ConA. These LTT modifications are not paralleled by quantitative variations of B-cells assessed by surface Ig immunofluorescent staining and EAC rosetting.", "contents": "Adjuvant-induced arthritis in four inbred strains of rats. An in vitro study of peripheral T and B lymphocytes. The lymphoblastic response (LTT) to non-specific mitogens (PHA, PWM and ConA) of peripheral lymphocytes was investigated at days 0, 7, 14, 21 and 28 after adjuvant injection in four strains of inbred rats: Wistar (WAG), Long Evans (LE), Lewis (LEW) and Brown Norway (BN). LTT was assessed by using 18 hours H3 TdR incorporation in 5 days cultures of whole blood (micromethod). The statistical treatment of data, using principal components multifactorial analysis and analysis of variance showed a striking difference between strains. In control animals the responses to PHA and PWM were correlated and were higher in LE and WAG than in LEW and BN (BN=LEW less than LE=WAG). The response to ConA was independent of that to the other mitogens. It was generally low, but significantly higher in LEW and BN than in WAG and LE. In adjuvant-injected animals the responses to PHA and PWM were still correlated, but modified compared to control: in LE and LEW, but not in WAG and BN, a marked decrease of the response was found, reaching a minimum value within days 7 and 14. In the same time the response to ConA increased in the four strains, later in LE than in the others. However the intensity of the ConA response varied from one strain to another: it was constantly low in LE and WAG compared to LEW and BN. So the most striking modification of LTT were observed in LE and LEW, which both developed the most severe arthritis. However these different behaviours after adjuvant injection were not explained by the initial level of LTT to the different mitogens. These data suggest that the development of intense arthritis is associated with the proliferation and the release into the blood stream of a lymphocyte subpopulation, which exhibits a low response to PHA and PWM and a high response to ConA. These LTT modifications are not paralleled by quantitative variations of B-cells assessed by surface Ig immunofluorescent staining and EAC rosetting."}
{"id": "PMID:1085091", "title": "[Protein study on perilymph susbstitution during cerebrospinal fluid flow through cochlear aqueduct].", "content": "Total protein contents in the perilymphy of Scala vestibuli and Scala tympani as well as in the cerobrospinal fluid (CSF) of guinea-pigs were determined, by which specimens were taken under the following various conditions: With or without subarachnoidal puncture before perilymph collection, by varying the amount of perilymph taken from Scala tympani, by fractionating collection and under post mortem condition. The results suggest that under physiological conditions the CSF also flows through the cochleae aqueduct and the protein concentration in the Scala tympani decreases especially in the basal winding. Because the protein content in CSF is inaverage four times lower than in fluids of the inner ear it is of preference to consider the question of connection between the CSF and the fluids of the inner ear. In the specimens taken the blood contamination was estimated on the basisof erythrocytes in the connection capillaries under microscope. The protein content in the perilymph of Scala vestibuli was found significantly higher in relation to perilymph of Scala tympani.", "contents": "[Protein study on perilymph susbstitution during cerebrospinal fluid flow through cochlear aqueduct]. Total protein contents in the perilymphy of Scala vestibuli and Scala tympani as well as in the cerobrospinal fluid (CSF) of guinea-pigs were determined, by which specimens were taken under the following various conditions: With or without subarachnoidal puncture before perilymph collection, by varying the amount of perilymph taken from Scala tympani, by fractionating collection and under post mortem condition. The results suggest that under physiological conditions the CSF also flows through the cochleae aqueduct and the protein concentration in the Scala tympani decreases especially in the basal winding. Because the protein content in CSF is inaverage four times lower than in fluids of the inner ear it is of preference to consider the question of connection between the CSF and the fluids of the inner ear. In the specimens taken the blood contamination was estimated on the basisof erythrocytes in the connection capillaries under microscope. The protein content in the perilymph of Scala vestibuli was found significantly higher in relation to perilymph of Scala tympani."}
{"id": "PMID:1085096", "title": "Unrelated delayed hypersensitivity reactions accelerate the recovery of immunological responsiveness of specifically depleted cell populations.", "content": "Normal CBA mouse spleen cells were specifically depleted of cells spontaneously reacting to pigeon erythrocytes (PRBC) by two methods, the first allows specific depletion of anti-PRBC thymus derived (T) rosette  forming cells (RFC) whereas the second depletes bone marrow derived (B) anti-PRBC hemolytic plaque forming cells (PFC). Depleted populations transferred into lethally irradiated syngeneic recipients and stimulated with PRBC failed to develop any significant response but they normally responded to a stimulation with sheep erythrocytes (SRBC). When spleen cells were taken from mice skin painted with picryl (trinitrophenyl: TNP) chloride 12 days before and the recipients were challenged in the same way and stimulated with PRBC, they become capable of producing a definite response to this antigen. Moreover in these animals, a consistent although low number of cells was found, which simultaneously reacted to both native PRBC and TNP conjugated SRBC. These findings show that unrelated delayed hypersensitivity reactions promote the immunological recovery of specifically depleted cell populations.", "contents": "Unrelated delayed hypersensitivity reactions accelerate the recovery of immunological responsiveness of specifically depleted cell populations. Normal CBA mouse spleen cells were specifically depleted of cells spontaneously reacting to pigeon erythrocytes (PRBC) by two methods, the first allows specific depletion of anti-PRBC thymus derived (T) rosette  forming cells (RFC) whereas the second depletes bone marrow derived (B) anti-PRBC hemolytic plaque forming cells (PFC). Depleted populations transferred into lethally irradiated syngeneic recipients and stimulated with PRBC failed to develop any significant response but they normally responded to a stimulation with sheep erythrocytes (SRBC). When spleen cells were taken from mice skin painted with picryl (trinitrophenyl: TNP) chloride 12 days before and the recipients were challenged in the same way and stimulated with PRBC, they become capable of producing a definite response to this antigen. Moreover in these animals, a consistent although low number of cells was found, which simultaneously reacted to both native PRBC and TNP conjugated SRBC. These findings show that unrelated delayed hypersensitivity reactions promote the immunological recovery of specifically depleted cell populations."}
{"id": "PMID:1085097", "title": "Factors which determine the accumulation of immunoblasts in gut and skin.", "content": "The capacity of immunoblasts from two sources (1) peripheral lymph nodes draining the site of application of a contact sensitizer and (2) mesenteric lymph nodes from mice infected with the gut parasite T. spiralis to migrate to the gut and to inflamed skin sites were compared. The peripheral lymph node blasts readily entered skin sites in a non-specific way but failed to migrate to the gut even when inflammation was induced. By contrast, the mesenteric lymph node blasts readily migrated to the gut in normal mice and in increased amounts to the gut of mice infected with T. spiralis or inflamed with oral turpentine. A small proportion of mesenteric lymph node blasts did, however, migrate, non-specifically to the skin but in much smaller amounts than peripheral lymph node blasts. We conclude that the migration of immunoblasts to the gut has some specificity related to the source from which the cells were taken but little specificity with regard to intraluminal antigen.", "contents": "Factors which determine the accumulation of immunoblasts in gut and skin. The capacity of immunoblasts from two sources (1) peripheral lymph nodes draining the site of application of a contact sensitizer and (2) mesenteric lymph nodes from mice infected with the gut parasite T. spiralis to migrate to the gut and to inflamed skin sites were compared. The peripheral lymph node blasts readily entered skin sites in a non-specific way but failed to migrate to the gut even when inflammation was induced. By contrast, the mesenteric lymph node blasts readily migrated to the gut in normal mice and in increased amounts to the gut of mice infected with T. spiralis or inflamed with oral turpentine. A small proportion of mesenteric lymph node blasts did, however, migrate, non-specifically to the skin but in much smaller amounts than peripheral lymph node blasts. We conclude that the migration of immunoblasts to the gut has some specificity related to the source from which the cells were taken but little specificity with regard to intraluminal antigen."}
{"id": "PMID:1085099", "title": "Autoantibodies in D-penicillamine treated rheumatoid arthritis.", "content": "The D-penicillamine (D-Pen.) treatment can induce some diseases accompanied by autoantibodies: lupus, pemphigus, myasthenia. The authors present the results of a systematic study of autoantibody occurrence during D-Pen.-treatment of the rheumatoid arthritis (RA): (1) Anti-nuclear antibodies are slightly positive in 34% of the untreated RA. They appear or enhance in 44% of the treated patients (22/50). They reach high titers (1/500) in two clinical-induced lupus and in three asymptomatic cases. (2) Anti-native DNA antibodies are not found in untreated patients. They become markedly enhanced in two clinical lupus as in three asymptomatic cases (3/50). (3) Anti-epidermal intercellular substance-antibodies are absent in non-treated as well as on pemphigus-free treated patients (0/40). They are slightly elevated in 5 induced pemphigus. (4) Anti-striated muscle antibodies are absent in non-treated patients, rarely and moderately elevated (3/40) in the asymptomatic D-Pen.-treated group. These findings could have a practical interest for the survey of the treatment.", "contents": "Autoantibodies in D-penicillamine treated rheumatoid arthritis. The D-penicillamine (D-Pen.) treatment can induce some diseases accompanied by autoantibodies: lupus, pemphigus, myasthenia. The authors present the results of a systematic study of autoantibody occurrence during D-Pen.-treatment of the rheumatoid arthritis (RA): (1) Anti-nuclear antibodies are slightly positive in 34% of the untreated RA. They appear or enhance in 44% of the treated patients (22/50). They reach high titers (1/500) in two clinical-induced lupus and in three asymptomatic cases. (2) Anti-native DNA antibodies are not found in untreated patients. They become markedly enhanced in two clinical lupus as in three asymptomatic cases (3/50). (3) Anti-epidermal intercellular substance-antibodies are absent in non-treated as well as on pemphigus-free treated patients (0/40). They are slightly elevated in 5 induced pemphigus. (4) Anti-striated muscle antibodies are absent in non-treated patients, rarely and moderately elevated (3/40) in the asymptomatic D-Pen.-treated group. These findings could have a practical interest for the survey of the treatment."}
{"id": "PMID:1085102", "title": "Fatal hemorrhage complicating carcinoma of the esophagus. Report of four cases.", "content": "Four cases of esophageal carcinoma complicated by fatal hemorrhage are reported. All four patients had recently completed radiation therapy. An aortoesophageal fistula was present in two cases; fibrinoid necrosis of the esophageal arteries was present in the other two. The esophageal tumor was localized in two cases and had disappeared in one case. In one patient it had metastasized widely. Ninety-nine other reports of esophageal cancer and fatal hemorrhage are reviewed from the literature. Aortoesophageal fistula was the cause of hemorrhage in 78 cases. Occlusion of the vasa vasorum by thrombosis, inflammation, neoplastic cells or radiation injury appears to be the cause of aortic necrosis and fistula formation. Prompt surgical approach, if possible, should be used to control hemorrhage, as the primary tumor may be localized to the esophagus only.", "contents": "Fatal hemorrhage complicating carcinoma of the esophagus. Report of four cases. Four cases of esophageal carcinoma complicated by fatal hemorrhage are reported. All four patients had recently completed radiation therapy. An aortoesophageal fistula was present in two cases; fibrinoid necrosis of the esophageal arteries was present in the other two. The esophageal tumor was localized in two cases and had disappeared in one case. In one patient it had metastasized widely. Ninety-nine other reports of esophageal cancer and fatal hemorrhage are reviewed from the literature. Aortoesophageal fistula was the cause of hemorrhage in 78 cases. Occlusion of the vasa vasorum by thrombosis, inflammation, neoplastic cells or radiation injury appears to be the cause of aortic necrosis and fistula formation. Prompt surgical approach, if possible, should be used to control hemorrhage, as the primary tumor may be localized to the esophagus only."}
{"id": "PMID:1085103", "title": "Mental retardation in a national population of young men in the Netherlands. II. Prevalence of mild mental retardation.", "content": "The prevalence of mild mental retardation in 19-year-old survivors of male births during 1944-1947 is derived from military records. The data are singular in that they are national, virtually complete for a total population of more than 400,000 men and include attributes not previously examined. They allow for the simultaneous use of three criteria: education (history of special schooling), psychometric (Raven intelligence test score), and clinical diagnosis (ICD (1948) 325.2, 325.3), which yielded rates per 1000 of 30,58 and 61, respectively. Rates by all three criteria varied in similar fashion with father's occupation and with religious affiliation. Rates by the psychometric and diagnostic criteria were higher for rural- and urban-born, while rates by the special schooling criterion were lower for rural-born. These variations are presumed to indicate deficiencies in rural provisions of special schooling on the one hand, and a substantive increment in \"true\" prevalence on the other. Marked variations in rates by province are not accounted for by social class and urban/rural birthplace. A rise in rates in the 1947 birth cohort on the psychometric and diagnostic criteria but not on the schooling criterion is attributable to a scoring change in the IQ criterion. Relative risks are estimated for the psychometric criterion of low Raven test score according to father's occupation, urban-rural origin, and religious affiliation. The overlap of identification among individuals designated by one, two, or three of the criteria is examined, and the issue of labeling is explored.", "contents": "Mental retardation in a national population of young men in the Netherlands. II. Prevalence of mild mental retardation. The prevalence of mild mental retardation in 19-year-old survivors of male births during 1944-1947 is derived from military records. The data are singular in that they are national, virtually complete for a total population of more than 400,000 men and include attributes not previously examined. They allow for the simultaneous use of three criteria: education (history of special schooling), psychometric (Raven intelligence test score), and clinical diagnosis (ICD (1948) 325.2, 325.3), which yielded rates per 1000 of 30,58 and 61, respectively. Rates by all three criteria varied in similar fashion with father's occupation and with religious affiliation. Rates by the psychometric and diagnostic criteria were higher for rural- and urban-born, while rates by the special schooling criterion were lower for rural-born. These variations are presumed to indicate deficiencies in rural provisions of special schooling on the one hand, and a substantive increment in \"true\" prevalence on the other. Marked variations in rates by province are not accounted for by social class and urban/rural birthplace. A rise in rates in the 1947 birth cohort on the psychometric and diagnostic criteria but not on the schooling criterion is attributable to a scoring change in the IQ criterion. Relative risks are estimated for the psychometric criterion of low Raven test score according to father's occupation, urban-rural origin, and religious affiliation. The overlap of identification among individuals designated by one, two, or three of the criteria is examined, and the issue of labeling is explored."}
{"id": "PMID:1085106", "title": "Alpha 1 antitrypsin M1: a new common genetically determined variant.", "content": "A new common variant (M1) of alpha 1 antitrypsin was detected by isoelectric focusing of serum in a pH gradient of 3.5-5.0 in polyacrylamide gels. The variant can be clearly distinguished from the common M type only when alpha 1 antitrypsin M is present in the same serum. It cannot be recognized on starch gel electrophoresis. The gene frequency in a population sample of United States whites was .09.", "contents": "Alpha 1 antitrypsin M1: a new common genetically determined variant. A new common variant (M1) of alpha 1 antitrypsin was detected by isoelectric focusing of serum in a pH gradient of 3.5-5.0 in polyacrylamide gels. The variant can be clearly distinguished from the common M type only when alpha 1 antitrypsin M is present in the same serum. It cannot be recognized on starch gel electrophoresis. The gene frequency in a population sample of United States whites was .09."}
{"id": "PMID:1085107", "title": "A behavioral classification of welfare children from survey data.", "content": "From survey data on 1000 urban Welfare AFDC children aged six to eighteen, a hierarchical cluster analysis yielded six distinct behavioral types of Welfare children. Characteristics of each type, and its relationship to treatment, ethnicity, and other variables are discussed. Advantages of this system of behavioral classification for research and population assessment are outlined.", "contents": "A behavioral classification of welfare children from survey data. From survey data on 1000 urban Welfare AFDC children aged six to eighteen, a hierarchical cluster analysis yielded six distinct behavioral types of Welfare children. Characteristics of each type, and its relationship to treatment, ethnicity, and other variables are discussed. Advantages of this system of behavioral classification for research and population assessment are outlined."}
{"id": "PMID:1085108", "title": "[Prevention of microaggregation in stored blood (author's transl)].", "content": "Microembolism is still a feared complication during and after blood transfusions. Up to now they could only be diminished by special filter systems. In spite of much research it has not been possible as yet to fine adequate remedies for the preservation of blood cells and for the decrease of aggregation in stored blood. As foregoing studies have shown the inhibition of aggregation is combined with a considerable impairment or even loss of platelet function. In these clinical experiments, however, it has been shown, that aggregations in stored blood are significantly stunted by early addition of Aprotinin. It is clinically important, that no inhibition of platelet function occured.", "contents": "[Prevention of microaggregation in stored blood (author's transl)]. Microembolism is still a feared complication during and after blood transfusions. Up to now they could only be diminished by special filter systems. In spite of much research it has not been possible as yet to fine adequate remedies for the preservation of blood cells and for the decrease of aggregation in stored blood. As foregoing studies have shown the inhibition of aggregation is combined with a considerable impairment or even loss of platelet function. In these clinical experiments, however, it has been shown, that aggregations in stored blood are significantly stunted by early addition of Aprotinin. It is clinically important, that no inhibition of platelet function occured."}
{"id": "PMID:1085114", "title": "[Histiocytosis X (author's transl)].", "content": "Two cases of histiocytosis X shown by clinical observation as forms of Abt-Letterer-Siwe are presented. Both have a high score following the scale on Lahey, according with the young age of the patients. Both had a very bad evolution. Treatment applied on each case, as well as the possibility and need to arrive at an early diagnosis is discussed.", "contents": "[Histiocytosis X (author's transl)]. Two cases of histiocytosis X shown by clinical observation as forms of Abt-Letterer-Siwe are presented. Both have a high score following the scale on Lahey, according with the young age of the patients. Both had a very bad evolution. Treatment applied on each case, as well as the possibility and need to arrive at an early diagnosis is discussed."}
{"id": "PMID:1085112", "title": "The local anesthetic activity of tetrodotoxin alone and in combination with vasoconstrictors and local anesthetics.", "content": "Tetrodotoxin (TTX), alone and in combination with various vasoconstrictors and local anesthetics, was evaluated for its ability to produce peripheral nerve blocks in the rat and central neural blocks in the cat and dog. High frequency and long duration of block can be attained if sufficiently high concentrations of TTX are used, although latency is long and high dosage may produce systemic toxicity. Frequency and mean duration of block can be increased and systemic toxicity reduced if TTX is administered with a vasoconstrictive agent. Conventional local anesthetics also enhance the nerve-blocking activity of TTX. When appropriate concentrations of TTX and local anesthetics are used, a high frequency of blocks characterized by short latency and long duration can be demonstrated. The studies present some indirect evidence that local anesthetics enhance TTX activity by reversibly increasing the permeability of various neural barriers to TTX.", "contents": "The local anesthetic activity of tetrodotoxin alone and in combination with vasoconstrictors and local anesthetics. Tetrodotoxin (TTX), alone and in combination with various vasoconstrictors and local anesthetics, was evaluated for its ability to produce peripheral nerve blocks in the rat and central neural blocks in the cat and dog. High frequency and long duration of block can be attained if sufficiently high concentrations of TTX are used, although latency is long and high dosage may produce systemic toxicity. Frequency and mean duration of block can be increased and systemic toxicity reduced if TTX is administered with a vasoconstrictive agent. Conventional local anesthetics also enhance the nerve-blocking activity of TTX. When appropriate concentrations of TTX and local anesthetics are used, a high frequency of blocks characterized by short latency and long duration can be demonstrated. The studies present some indirect evidence that local anesthetics enhance TTX activity by reversibly increasing the permeability of various neural barriers to TTX."}
{"id": "PMID:1085117", "title": "Aspects of treatment. The anatomical basis for portal decompressive surgery.", "content": "Definitive surgical techniques used in the treatment of portal hypertension all aim to achieve portal venous decompression. The historic development of the various procedures currently employed provides a broad repertoire of surgical operations. Each of the procedures merits consideration and may be specifically indicated in a given situation. Description of the operative steps emphasizes the importance of the anatomical relationships of the various structures.", "contents": "Aspects of treatment. The anatomical basis for portal decompressive surgery. Definitive surgical techniques used in the treatment of portal hypertension all aim to achieve portal venous decompression. The historic development of the various procedures currently employed provides a broad repertoire of surgical operations. Each of the procedures merits consideration and may be specifically indicated in a given situation. Description of the operative steps emphasizes the importance of the anatomical relationships of the various structures."}
{"id": "PMID:1085118", "title": "Anaesthesia for injection of bleeding oesophageal varices.", "content": "Patients with haemorrhage from oesophageal varices associated with portal hypertension are poor risks for anaesthesia and surgery. One method of controlling such haemorrhage is injection of the oesophageal varices (sclero-therapy) via an oesophagoscope. Careful preoperative preparation and use of the Sengstaken-Blakemore tube in combination with the anaesthetic technique of intermittent Althesin and suxamethonium with artificial ventilation with nitrous oxide and oxygen enables sclerotherapy to be carried out successfully.", "contents": "Anaesthesia for injection of bleeding oesophageal varices. Patients with haemorrhage from oesophageal varices associated with portal hypertension are poor risks for anaesthesia and surgery. One method of controlling such haemorrhage is injection of the oesophageal varices (sclero-therapy) via an oesophagoscope. Careful preoperative preparation and use of the Sengstaken-Blakemore tube in combination with the anaesthetic technique of intermittent Althesin and suxamethonium with artificial ventilation with nitrous oxide and oxygen enables sclerotherapy to be carried out successfully."}
{"id": "PMID:1085116", "title": "[2 cases of \"ganglionic sinusal histiocytosis\". A new term in cervical and rhino-pharyngo-laryngeal pathology?].", "content": "By 1974, Rosai, who is keeping a world-wide register of a condition described for the first time in France by Destombes, had collected 60 cases: the condition is called ganglionic sinusal lipid histiocytosis. The disease manifests itself principally in the form of adenopathies, usually cervical adenopathies, showing intermittent development over several years. It is rarely found in the viscera. The authors describe two cases, one typically confined to the ganglia, the other naso-ganglionic.", "contents": "[2 cases of \"ganglionic sinusal histiocytosis\". A new term in cervical and rhino-pharyngo-laryngeal pathology?]. By 1974, Rosai, who is keeping a world-wide register of a condition described for the first time in France by Destombes, had collected 60 cases: the condition is called ganglionic sinusal lipid histiocytosis. The disease manifests itself principally in the form of adenopathies, usually cervical adenopathies, showing intermittent development over several years. It is rarely found in the viscera. The authors describe two cases, one typically confined to the ganglia, the other naso-ganglionic."}
{"id": "PMID:1085120", "title": "Yeast-like fungi recovered from normal human skin in Nsukka (Nigeria).", "content": "The incidence of yeast-like fungi on normal skin was investigated in 52 healthy Nsukka individuals comprising 21 adults, 24 children, and 7 infants. Eighty-six isolates of yeasts and 2 of Geotrichum candidum were recovered. The yeasts included Candida tropicalis-36 isolates, C. guilliermondii-3, C. parapsilosis-2, Candida spp. (unidentified)-16, Cryptococcus albidus-6, Trichosporon cutaneum-7, Trichosporon spp.-8 and Rhodotorula spp.-8 isolates. The isolates originated from various sites, predominantly from nails and finger clefts. The significance of the findings is discussed with particular reference to the absence of C. albicans and the frequent isolation of C. tropicalis, T. cutaneum and C. albidus.", "contents": "Yeast-like fungi recovered from normal human skin in Nsukka (Nigeria). The incidence of yeast-like fungi on normal skin was investigated in 52 healthy Nsukka individuals comprising 21 adults, 24 children, and 7 infants. Eighty-six isolates of yeasts and 2 of Geotrichum candidum were recovered. The yeasts included Candida tropicalis-36 isolates, C. guilliermondii-3, C. parapsilosis-2, Candida spp. (unidentified)-16, Cryptococcus albidus-6, Trichosporon cutaneum-7, Trichosporon spp.-8 and Rhodotorula spp.-8 isolates. The isolates originated from various sites, predominantly from nails and finger clefts. The significance of the findings is discussed with particular reference to the absence of C. albicans and the frequent isolation of C. tropicalis, T. cutaneum and C. albidus."}
{"id": "PMID:1085115", "title": "[A biochemical and cytological explanation of cochlear otospongiosis].", "content": "Carrying on their study about the enzymatic activity of the otospongiotic micro-foci and the hydrolytic activity of the perilymph, the authors present their work concerning the value of trypsin and alpha-1-anti-trypsin in the perilymph of otospongiotic patients operated on by stapedectomy. They describe the method used and give the obtained results which permit to believe that the values of trypsin, and a contrario of anti-trypsin, appear to constitute an index of the severity of the progression. Moreover, the authors have investigated the eventual toxic action of various trypsin concentrations on the hair cells of the Corti organ in the guinea-pig. This study has been carried through successfully by means of the electron microscopic scanning method which allows to obtain an \"electron cochleogramme in situ\". They explain the method and come to the conclusion that the intensity and extension of the Corti hair cells alterations in a cochlea receiving a perfusion with trypsin solutions, are tightly related to the trypsin concentration. However, they do not pretend so far that these results reflect exactly the anatomo-pathologic reality during the slow progression of otospongiosis towards cochlear deterioration. These two series of experimentation appear to confirm their enzymatic concept of otospongiosis: the long, slow and extremely capricious progression of the disease is the duplicate of its cytologic progression, the contact between hydroltic enzymes and Corti cells having the same capricious character. These findings perfectly corroborate the previus ones they made in this field, as well as the correlations they have established between hydrolytic activity of the perilymph and audiometric progression. Consequently, they believe that the mechanism of the otospongiotic disease is provoked by the rupture of the balance trypsin/anti-trypsin in the otospongiotic micro-foci and thus in the inner ear fluids.", "contents": "[A biochemical and cytological explanation of cochlear otospongiosis]. Carrying on their study about the enzymatic activity of the otospongiotic micro-foci and the hydrolytic activity of the perilymph, the authors present their work concerning the value of trypsin and alpha-1-anti-trypsin in the perilymph of otospongiotic patients operated on by stapedectomy. They describe the method used and give the obtained results which permit to believe that the values of trypsin, and a contrario of anti-trypsin, appear to constitute an index of the severity of the progression. Moreover, the authors have investigated the eventual toxic action of various trypsin concentrations on the hair cells of the Corti organ in the guinea-pig. This study has been carried through successfully by means of the electron microscopic scanning method which allows to obtain an \"electron cochleogramme in situ\". They explain the method and come to the conclusion that the intensity and extension of the Corti hair cells alterations in a cochlea receiving a perfusion with trypsin solutions, are tightly related to the trypsin concentration. However, they do not pretend so far that these results reflect exactly the anatomo-pathologic reality during the slow progression of otospongiosis towards cochlear deterioration. These two series of experimentation appear to confirm their enzymatic concept of otospongiosis: the long, slow and extremely capricious progression of the disease is the duplicate of its cytologic progression, the contact between hydroltic enzymes and Corti cells having the same capricious character. These findings perfectly corroborate the previus ones they made in this field, as well as the correlations they have established between hydrolytic activity of the perilymph and audiometric progression. Consequently, they believe that the mechanism of the otospongiotic disease is provoked by the rupture of the balance trypsin/anti-trypsin in the otospongiotic micro-foci and thus in the inner ear fluids."}
{"id": "PMID:1085123", "title": "L-Sorbose metabolism in Agrobacterium tumefaciens.", "content": "The pathway of L-sorbose metabolism in Agrobacterium tumefaciens strain B6 was determined to be: L-sorbose leads to D-glucitol (sorbitol) leads to D-fructose leads to D-fructose-6-phosphate leads to D-glucose-6-phosphate. The reduction of L-sorbose and the oxidation of D-glucitol were mediated by NADPH- and NAD+-linked oxidoreductases, respectively. The intermediates, D-glucitol and D-fructose, were isolated from in vitro reaction mixtures by column chromatography on Dowex 1-borate, and identified enzymatically. D-Fructose was identified chemically by its 1H-NMR spectrum and the IR spectrum and the melting point of the fructosazone. D-Glucitol was characterized chemically by the melting point and the IR spectrum of its hexaacetate. A. tumefaciens ICPB TT111, a representative of another genetic race of Agrobacterium, lacked L-sorbose reductase and therefore failed to grow on L-sorbose; it grew normally on D-glucitol.", "contents": "L-Sorbose metabolism in Agrobacterium tumefaciens. The pathway of L-sorbose metabolism in Agrobacterium tumefaciens strain B6 was determined to be: L-sorbose leads to D-glucitol (sorbitol) leads to D-fructose leads to D-fructose-6-phosphate leads to D-glucose-6-phosphate. The reduction of L-sorbose and the oxidation of D-glucitol were mediated by NADPH- and NAD+-linked oxidoreductases, respectively. The intermediates, D-glucitol and D-fructose, were isolated from in vitro reaction mixtures by column chromatography on Dowex 1-borate, and identified enzymatically. D-Fructose was identified chemically by its 1H-NMR spectrum and the IR spectrum and the melting point of the fructosazone. D-Glucitol was characterized chemically by the melting point and the IR spectrum of its hexaacetate. A. tumefaciens ICPB TT111, a representative of another genetic race of Agrobacterium, lacked L-sorbose reductase and therefore failed to grow on L-sorbose; it grew normally on D-glucitol."}
{"id": "PMID:1085124", "title": "Nematophagous fungi: new species of the Lagenidiales endoparasitic on Rhabditis.", "content": "Two new species of the Lagenidiales endoparasitic on the nematode Rhabditis are reported from Ontario soils. In Myzocytium lenticulare the biflagellate zoospores encyst at any point on the cuticle of the host and penetrate directly through the wall. Sexual reproduction in this species is by oogamy. The antheridial protoplast migrates through a pore in the adjacent wall and fuses with the oogonial protoplast to produce a thick-walled reticulate oospore. In Lagenidium caudatum large biflagellate zoospores encyst at or near the body orifices of the host. Many of the zoospores possess a distinct tail-like appendage. Germination tubes from the encysted zoospores penetrate the body orifices to infect the host. The thallus in this species is distinctive in being composed of narrow, irregular hyphae which fill the host. No sexual stage is known.", "contents": "Nematophagous fungi: new species of the Lagenidiales endoparasitic on Rhabditis. Two new species of the Lagenidiales endoparasitic on the nematode Rhabditis are reported from Ontario soils. In Myzocytium lenticulare the biflagellate zoospores encyst at any point on the cuticle of the host and penetrate directly through the wall. Sexual reproduction in this species is by oogamy. The antheridial protoplast migrates through a pore in the adjacent wall and fuses with the oogonial protoplast to produce a thick-walled reticulate oospore. In Lagenidium caudatum large biflagellate zoospores encyst at or near the body orifices of the host. Many of the zoospores possess a distinct tail-like appendage. Germination tubes from the encysted zoospores penetrate the body orifices to infect the host. The thallus in this species is distinctive in being composed of narrow, irregular hyphae which fill the host. No sexual stage is known."}
{"id": "PMID:1085126", "title": "Differentiation of pathogenic species of Candida by their recovery characteristics following ultraviolet irradiation.", "content": "Each of seven pathogenic species of Candida exhibits a unique pattern of light and dark recovery responses to ultraviolet irradiation. C. guiliermondii, C. parapsilosis and C. pseudotropicalis photoreactivate whereas C. albicans, C. krusei, C. stellatoidea and C. tropicalis do not. Within each of these groups, individual species are distinguishable by whether or not they express differential dark recovery during postirradiation growth at 25 C or 37 C on oxidative vs fermentative carbon sources, on inorganic vs amino acid nitrogen sources or in the presence rather than absence of ergosterol. Equivalent recovery patterns are obtained for species of Candida and the ascosporogenous species which are their corresponding perfect forms. These observations indicate strongly that the post-irradation recovery is a reliable, species-specific characteristic of yeasts.", "contents": "Differentiation of pathogenic species of Candida by their recovery characteristics following ultraviolet irradiation. Each of seven pathogenic species of Candida exhibits a unique pattern of light and dark recovery responses to ultraviolet irradiation. C. guiliermondii, C. parapsilosis and C. pseudotropicalis photoreactivate whereas C. albicans, C. krusei, C. stellatoidea and C. tropicalis do not. Within each of these groups, individual species are distinguishable by whether or not they express differential dark recovery during postirradiation growth at 25 C or 37 C on oxidative vs fermentative carbon sources, on inorganic vs amino acid nitrogen sources or in the presence rather than absence of ergosterol. Equivalent recovery patterns are obtained for species of Candida and the ascosporogenous species which are their corresponding perfect forms. These observations indicate strongly that the post-irradation recovery is a reliable, species-specific characteristic of yeasts."}
{"id": "PMID:1085127", "title": "On the contribution of the acrylate pathway to the formation of propionate from lactate in the rumen of cattle.", "content": "The effect of chloral hydrate, an inhibitor of methanogenesis, on the participation of the acrylate pathway in the formation of propionate from lactate in rumen contents of cattle was studied in vitro. Addition of chloral hydrate resulted in only a small stimulation of the acrylate pathway, much lower than the stimulation of propionate production by chloral hydrate. This means that the flux of carbon through both the acrylate and the dicarboxylic acid pathway is increased during chloral hydrate feeding. The influence of time of sampling after feeding on the contribution of the acrylate pathway was studied in a separate experiment. A marked drop in the participation of the acrylate pathway in propionate formation from lactate during at least 2 h after feeding was observed, whereafter a rapid rise to prefeeding levels occurred.", "contents": "On the contribution of the acrylate pathway to the formation of propionate from lactate in the rumen of cattle. The effect of chloral hydrate, an inhibitor of methanogenesis, on the participation of the acrylate pathway in the formation of propionate from lactate in rumen contents of cattle was studied in vitro. Addition of chloral hydrate resulted in only a small stimulation of the acrylate pathway, much lower than the stimulation of propionate production by chloral hydrate. This means that the flux of carbon through both the acrylate and the dicarboxylic acid pathway is increased during chloral hydrate feeding. The influence of time of sampling after feeding on the contribution of the acrylate pathway was studied in a separate experiment. A marked drop in the participation of the acrylate pathway in propionate formation from lactate during at least 2 h after feeding was observed, whereafter a rapid rise to prefeeding levels occurred."}
{"id": "PMID:1085128", "title": "Purification and properties of two fructose-6-phosphate phosphoketolases in Bifidobacterium.", "content": "Fructose-6-phosphate phosphoketolase was purified from type strains of two species of the genus Bifidobacterium: B. globosum and B. dentium. The first species has a preferred \"animal\" habitat, like feces of animals and rumen of cattle; the latter is harboured in \"human\" habitat, like feces and dental caries of man. Two electrophoretic types of phosphoketolase (F6PPK) were previously distinguished and called \"animal\" and \"human\" type according to the habitat of the bifid organism. The purified preparations of these two phosphoketolases displayed very different optimum pH range, metal activator and molecular weight; outstanding difference was found in the substrate specificity: the enzyme from B. globosum was able to split xylulose-5-P as well as fructose-6-P, whereas the phosphoketolase from B. dentium appeared to be specific for fructose-6-P.", "contents": "Purification and properties of two fructose-6-phosphate phosphoketolases in Bifidobacterium. Fructose-6-phosphate phosphoketolase was purified from type strains of two species of the genus Bifidobacterium: B. globosum and B. dentium. The first species has a preferred \"animal\" habitat, like feces of animals and rumen of cattle; the latter is harboured in \"human\" habitat, like feces and dental caries of man. Two electrophoretic types of phosphoketolase (F6PPK) were previously distinguished and called \"animal\" and \"human\" type according to the habitat of the bifid organism. The purified preparations of these two phosphoketolases displayed very different optimum pH range, metal activator and molecular weight; outstanding difference was found in the substrate specificity: the enzyme from B. globosum was able to split xylulose-5-P as well as fructose-6-P, whereas the phosphoketolase from B. dentium appeared to be specific for fructose-6-P."}
{"id": "PMID:1085129", "title": "Oxidation of ethylene by soil bacteria.", "content": "The course of the biological oxidation of ethylene by soil was dependent on the type of soil used as well as on other factors. As evidenced from an increase in oxidation rate, the ethylene-consuming microorganisms in soil could grow at the expense of ethylene, even when the gas was present at concentrations of 50 ppm or less. Five strains of bacteria strongly resembling each other were isolated from different soils. These pleomorphic, gram-positive, acid-fast, obligate aerobic, ethylene-oxidizing bacteria grew also on saturated alkanes and on ordinary carbon sources. An apparent Km for ethylene of approximately 40 ppm was estimated for whole-cell suspensions of strain E20 by following the disappearance of the gas from the atmosphere.", "contents": "Oxidation of ethylene by soil bacteria. The course of the biological oxidation of ethylene by soil was dependent on the type of soil used as well as on other factors. As evidenced from an increase in oxidation rate, the ethylene-consuming microorganisms in soil could grow at the expense of ethylene, even when the gas was present at concentrations of 50 ppm or less. Five strains of bacteria strongly resembling each other were isolated from different soils. These pleomorphic, gram-positive, acid-fast, obligate aerobic, ethylene-oxidizing bacteria grew also on saturated alkanes and on ordinary carbon sources. An apparent Km for ethylene of approximately 40 ppm was estimated for whole-cell suspensions of strain E20 by following the disappearance of the gas from the atmosphere."}
{"id": "PMID:1085130", "title": "Antibacterial activity of delta9-tetrahydrocannabinol and cannabidiol.", "content": "The minimum inhibiting concentrations (MIC) of delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD) for staphylococci and streptococci in broth are in the range of 1-5 mug/ml. In the same range, both compounds are also bactericidal. In media containing 4% serum or 5% blood the antibacterial activity is strongly reduced (MIC 50 mug/ml). Gram-negative bacteria are resistant to THC and CBD.", "contents": "Antibacterial activity of delta9-tetrahydrocannabinol and cannabidiol. The minimum inhibiting concentrations (MIC) of delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD) for staphylococci and streptococci in broth are in the range of 1-5 mug/ml. In the same range, both compounds are also bactericidal. In media containing 4% serum or 5% blood the antibacterial activity is strongly reduced (MIC 50 mug/ml). Gram-negative bacteria are resistant to THC and CBD."}
{"id": "PMID:1085131", "title": "Immunologic responses in patients with lepromatous leprosy.", "content": "Immunologic responses were measured in 46 patients with lepromatous leprosy. These patients were not distinguishable from controls on the basis of responses to soluble intradermal antigens, sensitization to contactants, peripheral blood T- and B-cell percentages, in vitro lymphocyte responses to a mitogen, or the prevalence of autoantibodies. Generalized immunologic abnormalities in patients with lepromatous leprosy are neither predisposing causes nor necessary accompaniments of lepromatous leprosy, but are probably remote sequellae of the illness. By implication, the generalized immunologic abnormalities reported in other diseases are likely to be remote sequellae of the particular illness.", "contents": "Immunologic responses in patients with lepromatous leprosy. Immunologic responses were measured in 46 patients with lepromatous leprosy. These patients were not distinguishable from controls on the basis of responses to soluble intradermal antigens, sensitization to contactants, peripheral blood T- and B-cell percentages, in vitro lymphocyte responses to a mitogen, or the prevalence of autoantibodies. Generalized immunologic abnormalities in patients with lepromatous leprosy are neither predisposing causes nor necessary accompaniments of lepromatous leprosy, but are probably remote sequellae of the illness. By implication, the generalized immunologic abnormalities reported in other diseases are likely to be remote sequellae of the particular illness."}
{"id": "PMID:1085133", "title": "Administration of antirheumatic drugs.", "content": "A study of 200 rheumatic patients attending an outpatient clinic and 72 general practitioners (GPs) was undertaken in relation to the administration of antirheumatic drugs. (1) Both patients and GPs agreed that effectiveness, absence of toxicity, and once daily administration were the important features of administration. (2) Significant differences between GPs and patients were noted in that patients more frequently preferred capsules than tablets. (3) GPs thought red was the best colour for an antirheumatic tablet, whereas patients thought white, this opinion being partly determined by the possible confusion of red tablets with sweets by children. (4) In a survey of 174 outpatients with rheumatic diseases, those with rheumatoid arthritis did not like blister packaging. A detailed assessment of 30 patients with rheumatoid arthritis in hospital confirmed this. Patients with moderate or severe rheumatoid disease of the hands often could not extract tablets from blister packs. Those who could found the packs difficult to open, the tablets broke, and came out suddenly, falling to the floor.", "contents": "Administration of antirheumatic drugs. A study of 200 rheumatic patients attending an outpatient clinic and 72 general practitioners (GPs) was undertaken in relation to the administration of antirheumatic drugs. (1) Both patients and GPs agreed that effectiveness, absence of toxicity, and once daily administration were the important features of administration. (2) Significant differences between GPs and patients were noted in that patients more frequently preferred capsules than tablets. (3) GPs thought red was the best colour for an antirheumatic tablet, whereas patients thought white, this opinion being partly determined by the possible confusion of red tablets with sweets by children. (4) In a survey of 174 outpatients with rheumatic diseases, those with rheumatoid arthritis did not like blister packaging. A detailed assessment of 30 patients with rheumatoid arthritis in hospital confirmed this. Patients with moderate or severe rheumatoid disease of the hands often could not extract tablets from blister packs. Those who could found the packs difficult to open, the tablets broke, and came out suddenly, falling to the floor."}
{"id": "PMID:1085134", "title": "Relationship of phytohaemagglutinin-induced lymphocyte transformation to disease activity in systemic lupus erythematosus.", "content": "Phytohaemagglutinin-induced lymphocyte transformation was studied in 19 patients with systemic lupus erythematosus (SLE) in relation to disease activity, peripheral blood lymphocyte count, serum iron and folate levels, and corticosteroid treatment. Similar studies were performed on a group of 28 age- and sex-matched controls and on 10 patients with facial palsy who were examined before and after 7 days of high-dose corticosteroid treatment. The patients with SLE were found to have an impairment of lymphocyte transformation which was most marked in active stages of the disease and associated with a lymphopenia. This depressed transformation, which improved with the development of a remission, could not be attributed to the effects of corticosteroid treatment, inhibitory serum factors, iron deficiency, or any numerical reduction in blood lymphocytes, thus indicating along with evidence from other sources that SLE patients have a defect of cell-mediated immunity. The aetiological implications of these findings are discussed.", "contents": "Relationship of phytohaemagglutinin-induced lymphocyte transformation to disease activity in systemic lupus erythematosus. Phytohaemagglutinin-induced lymphocyte transformation was studied in 19 patients with systemic lupus erythematosus (SLE) in relation to disease activity, peripheral blood lymphocyte count, serum iron and folate levels, and corticosteroid treatment. Similar studies were performed on a group of 28 age- and sex-matched controls and on 10 patients with facial palsy who were examined before and after 7 days of high-dose corticosteroid treatment. The patients with SLE were found to have an impairment of lymphocyte transformation which was most marked in active stages of the disease and associated with a lymphopenia. This depressed transformation, which improved with the development of a remission, could not be attributed to the effects of corticosteroid treatment, inhibitory serum factors, iron deficiency, or any numerical reduction in blood lymphocytes, thus indicating along with evidence from other sources that SLE patients have a defect of cell-mediated immunity. The aetiological implications of these findings are discussed."}
{"id": "PMID:1085135", "title": "Drug-associated deaths of medical inpatients.", "content": "Of 7,423 medical inpatients, 16 (0.22%) died of drug-associated causes. The overall mortality for all medical inpatients was 6.5%. Eleven of the 16 patients who died of drug-associated causes had been terminally ill; the rest had been seriously ill before the fatal drug reaction occurred. Half of the patients had had either hematologic malignant changes or lupus nephritis. Antineoplastic drugs, azathioprine, prednisone, and heparin sodium were the most frequently implicated drugs. In other studies, we have found widely differing incidences of fatal drug reactions, due to a number of different drugs; these disparities are probably related to variations in the types of illnesses amoung different hospital populations and to varying interpretations of the term \"drug-associated death.\" Extrapolation from the available data to a national incidence of drug-associated deaths is not possible. Drug-associated deaths are relatively uncommon and usually occur in the cases of severely or terminally ill patients treated with potentially highly toxic drugs.", "contents": "Drug-associated deaths of medical inpatients. Of 7,423 medical inpatients, 16 (0.22%) died of drug-associated causes. The overall mortality for all medical inpatients was 6.5%. Eleven of the 16 patients who died of drug-associated causes had been terminally ill; the rest had been seriously ill before the fatal drug reaction occurred. Half of the patients had had either hematologic malignant changes or lupus nephritis. Antineoplastic drugs, azathioprine, prednisone, and heparin sodium were the most frequently implicated drugs. In other studies, we have found widely differing incidences of fatal drug reactions, due to a number of different drugs; these disparities are probably related to variations in the types of illnesses amoung different hospital populations and to varying interpretations of the term \"drug-associated death.\" Extrapolation from the available data to a national incidence of drug-associated deaths is not possible. Drug-associated deaths are relatively uncommon and usually occur in the cases of severely or terminally ill patients treated with potentially highly toxic drugs."}
{"id": "PMID:1085136", "title": "Aortoenteric fistula. A complication of renal artery bypass graft.", "content": "The incidence of gastrointestinal bleeding secondary to aortoenteric fistula has increased in recent years consequent to more frequent aortic reconstructive procedures. It is necessary to approach any such patient with this diagnostic consideration in mind, since early specific therapy may decrease the mortality. In this setting, there is usually sufficient time available to perform definitive tests to establish the correct diagnosis. We report a 37-year-old patient in whom aortoenteric fistula developed following a renal artery bypass graft.", "contents": "Aortoenteric fistula. A complication of renal artery bypass graft. The incidence of gastrointestinal bleeding secondary to aortoenteric fistula has increased in recent years consequent to more frequent aortic reconstructive procedures. It is necessary to approach any such patient with this diagnostic consideration in mind, since early specific therapy may decrease the mortality. In this setting, there is usually sufficient time available to perform definitive tests to establish the correct diagnosis. We report a 37-year-old patient in whom aortoenteric fistula developed following a renal artery bypass graft."}
{"id": "PMID:1085137", "title": "Rhodopseudomonas sphaeroides forma sp. denitrificans, a denitrifying strain as a subspecies of Rhodopseudomonas sphaeroides.", "content": "From polluted water of a lagoon pond a new type of denitrifying photosynthetic purple bacteria was isolated. With respect to morphology, fine structure, photopigments, requirement for growth factors, the range of utilization of organic substrates for phototrophic growth and DNA base ratio, the denitrifying strains show the closest resemblance to Rhodopseudomonas sphaeroides and were therefore described as a subspecies named R. sphaeroides forma sp. denitrificans. The new isolates grow well with nitrate anaerobically in the dark accompanying the evolution of nitrogen gas. They cannot assimilate nitrate as the nitrogen source for growth.", "contents": "Rhodopseudomonas sphaeroides forma sp. denitrificans, a denitrifying strain as a subspecies of Rhodopseudomonas sphaeroides. From polluted water of a lagoon pond a new type of denitrifying photosynthetic purple bacteria was isolated. With respect to morphology, fine structure, photopigments, requirement for growth factors, the range of utilization of organic substrates for phototrophic growth and DNA base ratio, the denitrifying strains show the closest resemblance to Rhodopseudomonas sphaeroides and were therefore described as a subspecies named R. sphaeroides forma sp. denitrificans. The new isolates grow well with nitrate anaerobically in the dark accompanying the evolution of nitrogen gas. They cannot assimilate nitrate as the nitrogen source for growth."}
{"id": "PMID:1085138", "title": "[Alpha-1 antitrypsin and idiopathic respiratory distress of the newborn infant. Study in serum and lungs].", "content": "Serum level of alpha-1-antitrypsin is significantly reduced in newborns with idiopathic respiratory distress when compared to matched controls. This decrease was predominantly due to the group of infants who died from I.R.D., provided that alpha-1-antitrypsin determination was carried out on the first day of life. Alpha-1-antitrypsin was demonstrable by indirect immunofluorescence in hyaline membranes whereas fluorescence was negative in the alveolae of infants who had no hyaline membranes.", "contents": "[Alpha-1 antitrypsin and idiopathic respiratory distress of the newborn infant. Study in serum and lungs]. Serum level of alpha-1-antitrypsin is significantly reduced in newborns with idiopathic respiratory distress when compared to matched controls. This decrease was predominantly due to the group of infants who died from I.R.D., provided that alpha-1-antitrypsin determination was carried out on the first day of life. Alpha-1-antitrypsin was demonstrable by indirect immunofluorescence in hyaline membranes whereas fluorescence was negative in the alveolae of infants who had no hyaline membranes."}
{"id": "PMID:1085140", "title": "Low-level dynamic exercises for earlier cardiac rehabilitation: aerobic and hemodynamic responses.", "content": "Three different kinds of low-level exercise (one arm and two leg exercises) and a low-level treadmill test were designed for patients recovering from acute myocardial infarction; noninvasive circulatory and aerobic responses to these activities were evaluated in healthy subjects. Exercise systolic pressure-rate product (X 10(-2)) were, respectively, 92 +/- 16, 98 +/- 10, 106 +/- 22 and 129 +/- 17 for the four activities studied. Exercise metabolic rates were 1.56 +/- 0.16, 1.99 +/- 0.22, 1.68 +/- 0.25 and 2.74 +/- 0.32 METS (multiples of the observed resting oxygen requirements). Additionally, arteriovenous oxygen difference, cardiac output, stroke volume, mean systemic and mean pulmonary pressures were measured in four coronary patients as they performed the leg exercises. It is hoped that this study will provide a basis for applying these different forms of exercise to patients recovering from an acute myocardial infarction, even when they are confined to bed, in order to advance the onset of cardiac rehabilitation. In a similar way, the new low-level treadmill test may be indicated for the same patients to assess the safety of discharging them from the hospital and permitting unsupervised ambulatory activity at home.", "contents": "Low-level dynamic exercises for earlier cardiac rehabilitation: aerobic and hemodynamic responses. Three different kinds of low-level exercise (one arm and two leg exercises) and a low-level treadmill test were designed for patients recovering from acute myocardial infarction; noninvasive circulatory and aerobic responses to these activities were evaluated in healthy subjects. Exercise systolic pressure-rate product (X 10(-2)) were, respectively, 92 +/- 16, 98 +/- 10, 106 +/- 22 and 129 +/- 17 for the four activities studied. Exercise metabolic rates were 1.56 +/- 0.16, 1.99 +/- 0.22, 1.68 +/- 0.25 and 2.74 +/- 0.32 METS (multiples of the observed resting oxygen requirements). Additionally, arteriovenous oxygen difference, cardiac output, stroke volume, mean systemic and mean pulmonary pressures were measured in four coronary patients as they performed the leg exercises. It is hoped that this study will provide a basis for applying these different forms of exercise to patients recovering from an acute myocardial infarction, even when they are confined to bed, in order to advance the onset of cardiac rehabilitation. In a similar way, the new low-level treadmill test may be indicated for the same patients to assess the safety of discharging them from the hospital and permitting unsupervised ambulatory activity at home."}
{"id": "PMID:1085141", "title": "Pancreatic abscess following acute pancreatitis.", "content": "Pancreatic abscess is probably the most serious complication of acute pancreatitis. During the ten-year period from 1966 to 1975, twenty-eight patients with pancreatic abscess following acute pancreatitis were treated by surgical drainage. A review of these cases revealed that there was a lull in the clinical course of the antecedent pancreatitis prior to the time of surgical drainage in 70% of the cases. Despite an aggressive surgical approach, there were major postoperative problems in 26 patients. Sepsis persisted in 14 patients. Major gastrointestinal hemorrhage occurred in seven, intra-abdominal bleeding in nine, and fistulization in 13. Fourteen patients died (a mortality of 50%). The operative treatment of pancreatic abscess must be aggressive and persistent. In addition to extensive drainage with soft sump drains, vigilance must be exercised to avoid pressure against bowel or major vessels. Reoperation should be considered if postoperative improvement is not sustained.", "contents": "Pancreatic abscess following acute pancreatitis. Pancreatic abscess is probably the most serious complication of acute pancreatitis. During the ten-year period from 1966 to 1975, twenty-eight patients with pancreatic abscess following acute pancreatitis were treated by surgical drainage. A review of these cases revealed that there was a lull in the clinical course of the antecedent pancreatitis prior to the time of surgical drainage in 70% of the cases. Despite an aggressive surgical approach, there were major postoperative problems in 26 patients. Sepsis persisted in 14 patients. Major gastrointestinal hemorrhage occurred in seven, intra-abdominal bleeding in nine, and fistulization in 13. Fourteen patients died (a mortality of 50%). The operative treatment of pancreatic abscess must be aggressive and persistent. In addition to extensive drainage with soft sump drains, vigilance must be exercised to avoid pressure against bowel or major vessels. Reoperation should be considered if postoperative improvement is not sustained."}
{"id": "PMID:1085142", "title": "Acute massive hemorrhage from intestinal Crohn disease. Report of seven cases and review of the literature.", "content": "Acute massive intestinal bleeding from Crohn disease occurred in 1.4% of 503 patients undergoing treatment at the New York Hospital-Cornell Medical Center over a 43-year period. Of the entire series, 31% had clinically evident rectal bleeding, while 13% had occult bleeding. Combined with previously reported series, onset of massive bleeding was not influenced by age of patient, duration of Crohn disease, use of corticosteroids, or activity of disease. Surgical therapy gave satisfactory results in patients with life-threatening hemorrhage.", "contents": "Acute massive hemorrhage from intestinal Crohn disease. Report of seven cases and review of the literature. Acute massive intestinal bleeding from Crohn disease occurred in 1.4% of 503 patients undergoing treatment at the New York Hospital-Cornell Medical Center over a 43-year period. Of the entire series, 31% had clinically evident rectal bleeding, while 13% had occult bleeding. Combined with previously reported series, onset of massive bleeding was not influenced by age of patient, duration of Crohn disease, use of corticosteroids, or activity of disease. Surgical therapy gave satisfactory results in patients with life-threatening hemorrhage."}
{"id": "PMID:1085143", "title": "Selective angiography. Inadequate guide for surgery in bleeding colonic diverticula.", "content": "We describe a case of severe bleeding from left-sided colonic diverticula, 2 1/2 years following right-sided colectomy for massive rectal bleeding confirmed angiographically to be from the right part of the colon. This experience demonstrates that total abdominal colectomy with ileoproctostomy is the only way to ensure that there is no bleeding postoperatively from diverticula, regardless of angiographic findings.", "contents": "Selective angiography. Inadequate guide for surgery in bleeding colonic diverticula. We describe a case of severe bleeding from left-sided colonic diverticula, 2 1/2 years following right-sided colectomy for massive rectal bleeding confirmed angiographically to be from the right part of the colon. This experience demonstrates that total abdominal colectomy with ileoproctostomy is the only way to ensure that there is no bleeding postoperatively from diverticula, regardless of angiographic findings."}
{"id": "PMID:1085139", "title": "[Interstitial pneumopathies in children treated for malignant diseases].", "content": "The authors report 21 cases of severe interstitial pneumonitis observed in 1974 in Paris, in children with malignancies, either solid tumours or leukemias. Twelve patients died. The incidence of these complications in children with intensive chemotherapy and the clinical symptoms which may reveal them are reviewed; the bad prognosis of measles in these patients is stressed. Radiological findings are described and the possible wrong diagnoses are listed. Among viral infections, measles is the severest and its diagnosis is often difficult. Pneumocystis carinii infection is frequent. The use of surgical pulmonary biopsy and other diagnostic procedures is discussed. The immune status of these patients has been studied, which revealed severe impairement of cellular immunity, including low lymphocyte count, while humoral immunity was not changed. Symptomatic treatment may include oxygen supply and mechanical respiratory help, dietary management, and attempts towards non specific immune system stimulation. Aetiologic treatment is essentially the treatment of Pneumocystis carinii, which is discussed. Diagnosis and treatment of pneumonitis in immunosuppressed patients being very difficult, emphasis is made on the prevention of this accident, including caution in the handling of chemotherapy protocols.", "contents": "[Interstitial pneumopathies in children treated for malignant diseases]. The authors report 21 cases of severe interstitial pneumonitis observed in 1974 in Paris, in children with malignancies, either solid tumours or leukemias. Twelve patients died. The incidence of these complications in children with intensive chemotherapy and the clinical symptoms which may reveal them are reviewed; the bad prognosis of measles in these patients is stressed. Radiological findings are described and the possible wrong diagnoses are listed. Among viral infections, measles is the severest and its diagnosis is often difficult. Pneumocystis carinii infection is frequent. The use of surgical pulmonary biopsy and other diagnostic procedures is discussed. The immune status of these patients has been studied, which revealed severe impairement of cellular immunity, including low lymphocyte count, while humoral immunity was not changed. Symptomatic treatment may include oxygen supply and mechanical respiratory help, dietary management, and attempts towards non specific immune system stimulation. Aetiologic treatment is essentially the treatment of Pneumocystis carinii, which is discussed. Diagnosis and treatment of pneumonitis in immunosuppressed patients being very difficult, emphasis is made on the prevention of this accident, including caution in the handling of chemotherapy protocols."}
{"id": "PMID:1085144", "title": "Immediate endoscopic diagnosis of upper gastrointestinal bleeding. Its accuracy and value in relation to associated pathology.", "content": "Two hundred sixty-two patients with active upper gastrointestinal (GI) bleeding underwent panendoscopy between July 1970 and March 1973. There was 100% accuracy of endoscopic diagnosis as to the anatomical site of bleeding; the etiopathologic definition was 94.7% accurate. The series was divided into two groups, 116 with \"liver disease\" and 146 with \"no liver disease.\" There were 107 patients with varices: 21 fell into no liver disease (small varices) and 86 into liver disease (39 small and 47 large varices). All had associated gastritis. Three endoscopic bleeding patterns were identified in the liver disease group. Only 27% of the patients in the liver disease group with varices (cirrhotics) had frank variceal hemorrhage, whereas 57% bled from hemorrhagic gastritis. The diagnostic unit provided early diagnosis, meaningful therapy, organized data gathering, and rough estimates of ultimate prognosis.", "contents": "Immediate endoscopic diagnosis of upper gastrointestinal bleeding. Its accuracy and value in relation to associated pathology. Two hundred sixty-two patients with active upper gastrointestinal (GI) bleeding underwent panendoscopy between July 1970 and March 1973. There was 100% accuracy of endoscopic diagnosis as to the anatomical site of bleeding; the etiopathologic definition was 94.7% accurate. The series was divided into two groups, 116 with \"liver disease\" and 146 with \"no liver disease.\" There were 107 patients with varices: 21 fell into no liver disease (small varices) and 86 into liver disease (39 small and 47 large varices). All had associated gastritis. Three endoscopic bleeding patterns were identified in the liver disease group. Only 27% of the patients in the liver disease group with varices (cirrhotics) had frank variceal hemorrhage, whereas 57% bled from hemorrhagic gastritis. The diagnostic unit provided early diagnosis, meaningful therapy, organized data gathering, and rough estimates of ultimate prognosis."}
{"id": "PMID:1085146", "title": "Human T lymphocyte colonies in agar: a comparison with other T cell assays in healthy subjects and cancer patients.", "content": "Colonies of human lymphocytes with T cell characteristics will grow in agar from repeated mitotic divisions with phytohaemagglutinin (PHA) stimulation. The colonies comprise spheres of tightly-packed cells with up to 500-1,000 blast-like cells in each colony. 65% of cells from pooled colonies bound AET-treated sheep red cells. 1,100-2,500 colonies/10(6) peripheral blood lymphocytes developed when cell donors were healthy but lower numbers (350-1,000 colonies/10(6) lymphocytes) were detected in blood from cancer patients. Comparison with other non-specific assays of cell-mediated immunity showed that while 66% of cancer patients were anergic (to five recall antigens) and 78% exhibited depressed mitotic activity in standard cultures with low dose PHA, 100% of these patients revealed T cell colony formation below normal. It is suggested that further studies of T lymphocyte colony-forming cells in healthy people and in a number of disease states may significantly advance our understanding of mechanisms of cell-mediated immunity.", "contents": "Human T lymphocyte colonies in agar: a comparison with other T cell assays in healthy subjects and cancer patients. Colonies of human lymphocytes with T cell characteristics will grow in agar from repeated mitotic divisions with phytohaemagglutinin (PHA) stimulation. The colonies comprise spheres of tightly-packed cells with up to 500-1,000 blast-like cells in each colony. 65% of cells from pooled colonies bound AET-treated sheep red cells. 1,100-2,500 colonies/10(6) peripheral blood lymphocytes developed when cell donors were healthy but lower numbers (350-1,000 colonies/10(6) lymphocytes) were detected in blood from cancer patients. Comparison with other non-specific assays of cell-mediated immunity showed that while 66% of cancer patients were anergic (to five recall antigens) and 78% exhibited depressed mitotic activity in standard cultures with low dose PHA, 100% of these patients revealed T cell colony formation below normal. It is suggested that further studies of T lymphocyte colony-forming cells in healthy people and in a number of disease states may significantly advance our understanding of mechanisms of cell-mediated immunity."}
{"id": "PMID:1085150", "title": "Effect of anti-inflammatory agent on L-glutamine-D-fructose-6-phosphate transaminase.", "content": "The enzyme L-glutamineD-fructose-6-phosphate aminotransferase (EC 2.6.1.16) was isolated, partially characterized, and purified from the gastric mucosa of dogs. A new method, using 14C-fructose-6-phosphate, has been developed for the estimation of the enzyme activity. Several classes of standard anti-inflammatory agents including acetyl-salicylic acid, salicylic acid, flufenamic acid, phenyl-butazone, indometacin, mefenamic acid, oxyphenyl-butazone, antipyrine, aminophenazone, allopurinol, cortisol, and dimethylsulfoxide (DMSO) were found to be inhibitors of this enzyme. The property of inhibition of this amino-transferase could, thus, be used as a rapid in vitro test method for the primary screening of potential anti-inflammatory agents.", "contents": "Effect of anti-inflammatory agent on L-glutamine-D-fructose-6-phosphate transaminase. The enzyme L-glutamineD-fructose-6-phosphate aminotransferase (EC 2.6.1.16) was isolated, partially characterized, and purified from the gastric mucosa of dogs. A new method, using 14C-fructose-6-phosphate, has been developed for the estimation of the enzyme activity. Several classes of standard anti-inflammatory agents including acetyl-salicylic acid, salicylic acid, flufenamic acid, phenyl-butazone, indometacin, mefenamic acid, oxyphenyl-butazone, antipyrine, aminophenazone, allopurinol, cortisol, and dimethylsulfoxide (DMSO) were found to be inhibitors of this enzyme. The property of inhibition of this amino-transferase could, thus, be used as a rapid in vitro test method for the primary screening of potential anti-inflammatory agents."}
{"id": "PMID:1085147", "title": "Lymphocytotoxic antibodies in SLE patients and their relatives.", "content": "A search for lymphocytotoxic antibodies in 50 SLE patients and in 109 of their blood relatives revealed an incidence of cold-reacting antibodies of 80% and 39% respectively, as compared with 15% in 110 normal blood donors paired for age, sex, and racial origin. The antibodies were also present in 7 of 18 (39%) of the spouses of SLE patients. Lymphocytotoxic antibodies exhibited broad reactivity in all groups, although positive SLE sera showed a tendency to react with a wider variety of lymphocytes and to present higher titers than positive sera from the other groups tested.", "contents": "Lymphocytotoxic antibodies in SLE patients and their relatives. A search for lymphocytotoxic antibodies in 50 SLE patients and in 109 of their blood relatives revealed an incidence of cold-reacting antibodies of 80% and 39% respectively, as compared with 15% in 110 normal blood donors paired for age, sex, and racial origin. The antibodies were also present in 7 of 18 (39%) of the spouses of SLE patients. Lymphocytotoxic antibodies exhibited broad reactivity in all groups, although positive SLE sera showed a tendency to react with a wider variety of lymphocytes and to present higher titers than positive sera from the other groups tested."}
{"id": "PMID:1085148", "title": "Serum antibody in rheumatoid arthritis reactive with a cell-associated antigen. Demonstration by precipitation and immunofluorescence.", "content": "A cell-free extract of human diploid B lymphocytes (Wil2) in continuous culture was used as the source of antigen in immunodiffusion to detect a precipitating antibody referred to as rheumatoid arthritis precipitin (RAP) in sera of patients with rheumatoid arthritis (RA). The prevalence of RAP was determined in various arthritides and other connective tissue diseases. It was found in 67% of patients with seropositive RA and in 62% of patients with Sj\u00f6gren's syndrome associated with RA. But its frequency was lower in many other connective tissue diseases, including seronegative RA and Sj\u00f6gren's syndrome without associated RA. Matching sera and synovial fluids from patients with seropositive RA were also studied. Differences in serum and synovial fluid RAP titers were demonstrated, but generally when RAP was present in serum, it was also present in synovial fluid and vice versa. Indirect immunofluorescence (IF) with Wil2 cells as substrate was used to demonstrate RAP and to determine morphologically the nature of the reactive cellular antigen. When cells were treated with commonly used fixatives, little or no staining with RAP positive sera was observed. This outcome was shown to be the result of the extreme solubility of the cellular antigen reactive with RAP. The antigen was retained best in a reactive form in Wil2 cells after fixation of cells with dry heat at 37 degrees C for 30 minutes, and it was demonstrated by IF staining as small round granules distributed in the nucleus and cytoplasm. IF was also employed to demonstrate that RAP is immunoglobulin belonging to IgG and IgM classes and not to IgA or IgD.", "contents": "Serum antibody in rheumatoid arthritis reactive with a cell-associated antigen. Demonstration by precipitation and immunofluorescence. A cell-free extract of human diploid B lymphocytes (Wil2) in continuous culture was used as the source of antigen in immunodiffusion to detect a precipitating antibody referred to as rheumatoid arthritis precipitin (RAP) in sera of patients with rheumatoid arthritis (RA). The prevalence of RAP was determined in various arthritides and other connective tissue diseases. It was found in 67% of patients with seropositive RA and in 62% of patients with Sj\u00f6gren's syndrome associated with RA. But its frequency was lower in many other connective tissue diseases, including seronegative RA and Sj\u00f6gren's syndrome without associated RA. Matching sera and synovial fluids from patients with seropositive RA were also studied. Differences in serum and synovial fluid RAP titers were demonstrated, but generally when RAP was present in serum, it was also present in synovial fluid and vice versa. Indirect immunofluorescence (IF) with Wil2 cells as substrate was used to demonstrate RAP and to determine morphologically the nature of the reactive cellular antigen. When cells were treated with commonly used fixatives, little or no staining with RAP positive sera was observed. This outcome was shown to be the result of the extreme solubility of the cellular antigen reactive with RAP. The antigen was retained best in a reactive form in Wil2 cells after fixation of cells with dry heat at 37 degrees C for 30 minutes, and it was demonstrated by IF staining as small round granules distributed in the nucleus and cytoplasm. IF was also employed to demonstrate that RAP is immunoglobulin belonging to IgG and IgM classes and not to IgA or IgD."}
{"id": "PMID:1085149", "title": "Lymphocyte populations in rheumatoid arthritis.", "content": "For each of 28 patients with RA and 27 normal controls, the in vitro lymphocyte responses to PHA and to Con A were simultaneously determined on aliquots of the same cell preparation. In the case of RA lymphocytes, the response to Con A was abnormally low when compared with the simultaneous response to PHA (P less than 0.01). Cells capable of responding to PHA were specifically killed with BUdR. The cells remaining after this procedure showed a relatively greater response to Con A than did the original population. In the case of RA lymphocytes, this relative increase in the response to Con A was significantly less than normal (0.02 greater than P greater than 0.01). These results suggest that a lymphocyte subpopulation, responding principally to Con A, is relatively reduced in RA.", "contents": "Lymphocyte populations in rheumatoid arthritis. For each of 28 patients with RA and 27 normal controls, the in vitro lymphocyte responses to PHA and to Con A were simultaneously determined on aliquots of the same cell preparation. In the case of RA lymphocytes, the response to Con A was abnormally low when compared with the simultaneous response to PHA (P less than 0.01). Cells capable of responding to PHA were specifically killed with BUdR. The cells remaining after this procedure showed a relatively greater response to Con A than did the original population. In the case of RA lymphocytes, this relative increase in the response to Con A was significantly less than normal (0.02 greater than P greater than 0.01). These results suggest that a lymphocyte subpopulation, responding principally to Con A, is relatively reduced in RA."}
{"id": "PMID:1085160", "title": "Autoantibodies in patients with chronic bronchitis.", "content": "Antinuclear antibody (ANA) was found in low titre in 28% of a group of 50 patients with chronic bronchitis by MRC criteria and defined as \"severe\", that is having at least six infective episodes a year, a vital capacity of less than 70% of predicted and less than 30% reversibility of their airways obstruction after inhalation of isoprenaline. By contrast ANA was found in only 4% of age and sex matched non-bronchitic controls and in none of 28 patients with \"less severe\" chronic bronchitis in whom these three criteria of severity were not fulfilled. The presence of antinuclear antibody was unrelated to the duration of the disease, sputum volume or the presence of purulent sputum at the time of the study. Neither was it related to blood eosinophilia, respiratory function, smoking or the presence of bacterial precipitins in the patient's serum. These findings are important because they suggest that incidental chronic bronchitis, unless it is extremely severe, is unlikely to contribute substantially to the prevalence of antinuclear antibody found in certain other lung diseases. There was no significant increase in smooth muscle or \"reticulin\" antibodies in this series of patients.", "contents": "Autoantibodies in patients with chronic bronchitis. Antinuclear antibody (ANA) was found in low titre in 28% of a group of 50 patients with chronic bronchitis by MRC criteria and defined as \"severe\", that is having at least six infective episodes a year, a vital capacity of less than 70% of predicted and less than 30% reversibility of their airways obstruction after inhalation of isoprenaline. By contrast ANA was found in only 4% of age and sex matched non-bronchitic controls and in none of 28 patients with \"less severe\" chronic bronchitis in whom these three criteria of severity were not fulfilled. The presence of antinuclear antibody was unrelated to the duration of the disease, sputum volume or the presence of purulent sputum at the time of the study. Neither was it related to blood eosinophilia, respiratory function, smoking or the presence of bacterial precipitins in the patient's serum. These findings are important because they suggest that incidental chronic bronchitis, unless it is extremely severe, is unlikely to contribute substantially to the prevalence of antinuclear antibody found in certain other lung diseases. There was no significant increase in smooth muscle or \"reticulin\" antibodies in this series of patients."}
{"id": "PMID:1085161", "title": "Diagnostic features of the Favre-Goldmann syndrome.", "content": "Extensive retinal vascular disease was noted in three patients from two families with Favre-Goldmann syndrome. In addition to classical features they had pronounced leakage from some retinal vessels. Vessels were either opaque ('sclerotic') or non-perfused. Cystoid macular oedema was a contributing cause of decreased vision. Two of the three patients showed a discrepancy on electroretinography between single-flash photopic amplitudes and flicker fusion frequency. This may be characteristic of Favre-Goldmann syndrome.", "contents": "Diagnostic features of the Favre-Goldmann syndrome. Extensive retinal vascular disease was noted in three patients from two families with Favre-Goldmann syndrome. In addition to classical features they had pronounced leakage from some retinal vessels. Vessels were either opaque ('sclerotic') or non-perfused. Cystoid macular oedema was a contributing cause of decreased vision. Two of the three patients showed a discrepancy on electroretinography between single-flash photopic amplitudes and flicker fusion frequency. This may be characteristic of Favre-Goldmann syndrome."}
{"id": "PMID:1085163", "title": "Pyrrolo[1,4]benzodiazepine antibiotics. Biosynthesis of the antitumor antibiotic 11-demethyltomaymycin and its biologically inactive metabolite oxotomaymycin by Streptomyces achromogenes.", "content": "11-Demethyltomaymycin, an antitumor antibiotic produced by Streptomyces achromogenes, and its biologically inactive metabolite oxotomaymycin are biosynthesized from L-tyrosine, DL-tryptophan, and L-methionine. The anthranilate part of 11-demethyltomaymycin is derived from tryptophan probably via the kynurenine pathway. The predominant loss of tritium from DL-[5-3H]tryptophan, during its conversion to 11-demethyltomaymycin and oxotomaymycin is interpreted to mean by NIH shift rules, that the main pathway to the 5-methoxy-4-hydroxy anthranilate moiety is through hydroxylation at C-8 prior to hydroxylation at C-7. The methoxy carbon is derived from the S-methyl group of methionine by transfer of an intact methyl group. The ethylideneproline moiety of 11-demethyltomaymycin is biosynthesized from tyrosine, without a 1-carbon unit from methionine. The results of biosynthetic feeding experiments with L-[1-14C, 3- or 5-3H]tyrosine are consistent with a \"meta\" or extradiol cleavage of 6,7-dihydroxycyclodopa as has also been demonstrated previously for anthramycin and lincomycin A. An experiment in which L-[1-14C, Ala-2,3-3H]tyrosine was fed showed that both the beta hydrogens of this amino acids are retained in 11-demethyltomaymycin. It has been demonstrated in cultures and washed cell preparations that 11-demethyltomaymycin is enzymatically converted to oxotomaymycin by an intracellular constitutive enzyme. Conversion of oxotomaymycin to 11-demethyltomaymycin by these same preparations could not be demonstrated. The enzymatic activity associated with the conversion of 11-demethyltomaymycin to oxotomaymycin is not limited to the 11-demethyltomaymycin to oxotomaymycin is not limited to the 11-demethyltomaymycin production phase, since trophophase cells and even cells from 11-demethyltomaymycin nonproducing cultures of S. achromogenes were equally active in converting 11-demethyltomaymycin to oxotomaymycin.", "contents": "Pyrrolo[1,4]benzodiazepine antibiotics. Biosynthesis of the antitumor antibiotic 11-demethyltomaymycin and its biologically inactive metabolite oxotomaymycin by Streptomyces achromogenes. 11-Demethyltomaymycin, an antitumor antibiotic produced by Streptomyces achromogenes, and its biologically inactive metabolite oxotomaymycin are biosynthesized from L-tyrosine, DL-tryptophan, and L-methionine. The anthranilate part of 11-demethyltomaymycin is derived from tryptophan probably via the kynurenine pathway. The predominant loss of tritium from DL-[5-3H]tryptophan, during its conversion to 11-demethyltomaymycin and oxotomaymycin is interpreted to mean by NIH shift rules, that the main pathway to the 5-methoxy-4-hydroxy anthranilate moiety is through hydroxylation at C-8 prior to hydroxylation at C-7. The methoxy carbon is derived from the S-methyl group of methionine by transfer of an intact methyl group. The ethylideneproline moiety of 11-demethyltomaymycin is biosynthesized from tyrosine, without a 1-carbon unit from methionine. The results of biosynthetic feeding experiments with L-[1-14C, 3- or 5-3H]tyrosine are consistent with a \"meta\" or extradiol cleavage of 6,7-dihydroxycyclodopa as has also been demonstrated previously for anthramycin and lincomycin A. An experiment in which L-[1-14C, Ala-2,3-3H]tyrosine was fed showed that both the beta hydrogens of this amino acids are retained in 11-demethyltomaymycin. It has been demonstrated in cultures and washed cell preparations that 11-demethyltomaymycin is enzymatically converted to oxotomaymycin by an intracellular constitutive enzyme. Conversion of oxotomaymycin to 11-demethyltomaymycin by these same preparations could not be demonstrated. The enzymatic activity associated with the conversion of 11-demethyltomaymycin to oxotomaymycin is not limited to the 11-demethyltomaymycin to oxotomaymycin is not limited to the 11-demethyltomaymycin production phase, since trophophase cells and even cells from 11-demethyltomaymycin nonproducing cultures of S. achromogenes were equally active in converting 11-demethyltomaymycin to oxotomaymycin."}
{"id": "PMID:1085164", "title": "The effect of ethanol on ion transport in frog skin.", "content": "Frog skin has been used as a model epithelial sodium-transporting system to study the effect of ethanol on ion transport. Treatment of the outside of frog skin with ethanol decreased the net sodium transport due to inhibition of 22Na+ influx. Ethanol did not alter sodium outflux when bathin the outside of the skin. The inhibition was in proportion to the concentration of ethanol, 0.25 M resulting in 50% inhibition. The chloride permeability of the skin was increased several-fold when the skin was exposed to ethanol in either bathing solution. With 0.4 M ethanol in the inner bathing solution, all the unidirectional fluxes of Na+ and C1- were increased. The movement of C1- was evaluated by comparison of C1- flux with urea flux, since urea is thought to move passively across frog skin via an extracellular (shunt) pathway. Chloride flux was increased to a greater extent than urea flux. These experiments indicate that ethanol affects chloride permeability beyond an increase in extracellular ion flow and independent of its effect of Na+ transport.", "contents": "The effect of ethanol on ion transport in frog skin. Frog skin has been used as a model epithelial sodium-transporting system to study the effect of ethanol on ion transport. Treatment of the outside of frog skin with ethanol decreased the net sodium transport due to inhibition of 22Na+ influx. Ethanol did not alter sodium outflux when bathin the outside of the skin. The inhibition was in proportion to the concentration of ethanol, 0.25 M resulting in 50% inhibition. The chloride permeability of the skin was increased several-fold when the skin was exposed to ethanol in either bathing solution. With 0.4 M ethanol in the inner bathing solution, all the unidirectional fluxes of Na+ and C1- were increased. The movement of C1- was evaluated by comparison of C1- flux with urea flux, since urea is thought to move passively across frog skin via an extracellular (shunt) pathway. Chloride flux was increased to a greater extent than urea flux. These experiments indicate that ethanol affects chloride permeability beyond an increase in extracellular ion flow and independent of its effect of Na+ transport."}
{"id": "PMID:1085165", "title": "Pysicochemical studies of taste reception. V. Suppressive effect of salts on sugar response of the frog.", "content": "The tast responses of frog to various kinds of sugars were measured quantitatively by use of the glossopharyngeal nerve activity under an appropriate condition where the water response was completely suppressed. The concentration dependences of response of frog tongue to D-fructose, D-glucose, and sucrose were almost the same, D-galactose, however, elicited a much larger response in comparison with the other sugars in the whole range of concentrations examined. The sugar response was suppressed extensively by the presence of small amount of salts in the stimulating sugar solution. The suppressive effects of NaCl, KCl, MgCl2, MgSO4, and K4Fe(CN)6 were examined with a fixed concentration of sugar. The results obtained with these salts, added in various concentrations, fell on a single curve when the data were plotted against the ionic strength in the stimulating solution. The present results were consistent with the notion that the taste receptor potential for salts or acids is attributable to a change in the phase boundary potential at the membrane-solution interface as proposed in the previous papers of this series.", "contents": "Pysicochemical studies of taste reception. V. Suppressive effect of salts on sugar response of the frog. The tast responses of frog to various kinds of sugars were measured quantitatively by use of the glossopharyngeal nerve activity under an appropriate condition where the water response was completely suppressed. The concentration dependences of response of frog tongue to D-fructose, D-glucose, and sucrose were almost the same, D-galactose, however, elicited a much larger response in comparison with the other sugars in the whole range of concentrations examined. The sugar response was suppressed extensively by the presence of small amount of salts in the stimulating sugar solution. The suppressive effects of NaCl, KCl, MgCl2, MgSO4, and K4Fe(CN)6 were examined with a fixed concentration of sugar. The results obtained with these salts, added in various concentrations, fell on a single curve when the data were plotted against the ionic strength in the stimulating solution. The present results were consistent with the notion that the taste receptor potential for salts or acids is attributable to a change in the phase boundary potential at the membrane-solution interface as proposed in the previous papers of this series."}
{"id": "PMID:1085166", "title": "Phytohaemagglutinin stimulation of rat thymus lymphocytes glycolysis.", "content": "Glucose disappearance and lactate production by the rat thymocytes are stimulated significantly 45 min after addition of phytohaemagglutinin or concanavalin A and the stimulated rate is sustained for at least 8 h. Changes in the steady-state concentration of glycolytic intermediates that occur at non-equilibrium steps during the increased rate of glycolytic flux indicate that the glucose carrier, hexokinase and phosphofructokinase are potentially regulatory steps that undergo nearly simultaneous or tightly sequential activation following interaction of the cells with the mitogen.", "contents": "Phytohaemagglutinin stimulation of rat thymus lymphocytes glycolysis. Glucose disappearance and lactate production by the rat thymocytes are stimulated significantly 45 min after addition of phytohaemagglutinin or concanavalin A and the stimulated rate is sustained for at least 8 h. Changes in the steady-state concentration of glycolytic intermediates that occur at non-equilibrium steps during the increased rate of glycolytic flux indicate that the glucose carrier, hexokinase and phosphofructokinase are potentially regulatory steps that undergo nearly simultaneous or tightly sequential activation following interaction of the cells with the mitogen."}
{"id": "PMID:1085167", "title": "Purification and physicochemical properties of superoxide dismutase from two photosynthetic microorganisms.", "content": "Superoxide dismutase (EC 1.15.1.1) has been isolated and characterised from the blue-green alga Spirulina platensis and from aerobically-grown Rhodopseudomonas spheroides, a purple, non-sulphur bacterium. The former enzyme contains 1 gatom of iron and the latter 1 gatom of manganese per mol; both enzymes have a molecular weight of 37 000-38 000, being composed of two non-covalently joined subunits of equal size. Various spectral studies have been carried out including absorbance, circular dichroism and electron spin resonance. Catalytic activity has been studied as a function of pH and shows a decrease at alkaline pH values. The manganoenzyme is generally more stable to various potentially denaturing conditions and is resistant to inactivation by hydrogen peroxide. Amino acid compositions and N-terminal residue determinations are presented.", "contents": "Purification and physicochemical properties of superoxide dismutase from two photosynthetic microorganisms. Superoxide dismutase (EC 1.15.1.1) has been isolated and characterised from the blue-green alga Spirulina platensis and from aerobically-grown Rhodopseudomonas spheroides, a purple, non-sulphur bacterium. The former enzyme contains 1 gatom of iron and the latter 1 gatom of manganese per mol; both enzymes have a molecular weight of 37 000-38 000, being composed of two non-covalently joined subunits of equal size. Various spectral studies have been carried out including absorbance, circular dichroism and electron spin resonance. Catalytic activity has been studied as a function of pH and shows a decrease at alkaline pH values. The manganoenzyme is generally more stable to various potentially denaturing conditions and is resistant to inactivation by hydrogen peroxide. Amino acid compositions and N-terminal residue determinations are presented."}
{"id": "PMID:1085168", "title": "Membranes of Rhodopseudomonas sphaeroides. IV. Assembly of chromatophores in low-aeration cell suspensions.", "content": "Chromatophore membrane formation was induced in low-aeration suspensions of Rhodopseudomonas sphaeroides and highly purified chromatophore preparations were isolated at various intervals between 4 and 18 h. The levels of several functional components associated with the isolated strucures were investigated. B-875, the light-harvesting bacteriochlorophyll complex associated with the reaction center, was preferentially inserted into the chromatophore membrane during the early stages of induction, and thereafter its levels reached a steady state; b- and c-type cytochromes were also maintained at essentially constant levels. In contrast, the levels of B-850, the accessory light-harvesting bacteriochlorophyll, together with its associated protein, continued to increase throughout the induction process. Increases in the levels of the major carotenoid component followed a similar course. These findings are consistent with a stepwise assembly mechanism for associated bacteriochlorophyll and protein components and suggest that separate regulatory mechanisms control the levels of functionally essential and accessory components within the membrane.", "contents": "Membranes of Rhodopseudomonas sphaeroides. IV. Assembly of chromatophores in low-aeration cell suspensions. Chromatophore membrane formation was induced in low-aeration suspensions of Rhodopseudomonas sphaeroides and highly purified chromatophore preparations were isolated at various intervals between 4 and 18 h. The levels of several functional components associated with the isolated strucures were investigated. B-875, the light-harvesting bacteriochlorophyll complex associated with the reaction center, was preferentially inserted into the chromatophore membrane during the early stages of induction, and thereafter its levels reached a steady state; b- and c-type cytochromes were also maintained at essentially constant levels. In contrast, the levels of B-850, the accessory light-harvesting bacteriochlorophyll, together with its associated protein, continued to increase throughout the induction process. Increases in the levels of the major carotenoid component followed a similar course. These findings are consistent with a stepwise assembly mechanism for associated bacteriochlorophyll and protein components and suggest that separate regulatory mechanisms control the levels of functionally essential and accessory components within the membrane."}
{"id": "PMID:1085169", "title": "The serum sialyltransferase activity in alpha 1-antitrypsin deficiency.", "content": "alpha 1-Antitrypsin phenotypes Pi M and Z, purified by the thiol-disulfide exchange procedure, were desialylated by treatment with neuraminidase covalently coupled to Sepharose and used as acceptors of sialic acid in an assay system for serum sialic acid transferase (CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) activity. Both asialoantitrypsins were equally effective as acceptors in contrast to native Pi Z antitrypsin which did not accept any sialic acid. Serum sialyltransferase activity was determined in 38 adult alpha 1-antitrypsin deficient individuals (Pi Z, MZ, FZ, SZ) with normal liver function and was found to be of the same magnitude as the activity in normal individuals (Pi M). Equal activities were also found in 5 Pi Z patients with cirrhosis of the liver. The results strongly argue against the concept that sialyltransferase deficiency provides the molecular basis for alpha 1-antitrypsin deficiency.", "contents": "The serum sialyltransferase activity in alpha 1-antitrypsin deficiency. alpha 1-Antitrypsin phenotypes Pi M and Z, purified by the thiol-disulfide exchange procedure, were desialylated by treatment with neuraminidase covalently coupled to Sepharose and used as acceptors of sialic acid in an assay system for serum sialic acid transferase (CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) activity. Both asialoantitrypsins were equally effective as acceptors in contrast to native Pi Z antitrypsin which did not accept any sialic acid. Serum sialyltransferase activity was determined in 38 adult alpha 1-antitrypsin deficient individuals (Pi Z, MZ, FZ, SZ) with normal liver function and was found to be of the same magnitude as the activity in normal individuals (Pi M). Equal activities were also found in 5 Pi Z patients with cirrhosis of the liver. The results strongly argue against the concept that sialyltransferase deficiency provides the molecular basis for alpha 1-antitrypsin deficiency."}
{"id": "PMID:1085170", "title": "Chronic hepatotoxicity and intestinal bleeding in 1,2-dimethylhydrazine carcinogenesis in rats and mice.", "content": "The long term administration of dimethylhydrazine to mice induces severe hepatotoxic damage. Parenchymal cell pleomorphism and necrosis were later associated with reticulin collapse and bile duct proliferation. Withdrawing the drug after 17 weeks was followed by some improvement in the parenchymal cells but there was a chronic proliferative response tending towards early cirrhotic changes. The rats appeared relatively resistant to the hepatotoxic effects but unlike the mice the colon tumors arising in these animals bled frequently and could result in profound anemia.", "contents": "Chronic hepatotoxicity and intestinal bleeding in 1,2-dimethylhydrazine carcinogenesis in rats and mice. The long term administration of dimethylhydrazine to mice induces severe hepatotoxic damage. Parenchymal cell pleomorphism and necrosis were later associated with reticulin collapse and bile duct proliferation. Withdrawing the drug after 17 weeks was followed by some improvement in the parenchymal cells but there was a chronic proliferative response tending towards early cirrhotic changes. The rats appeared relatively resistant to the hepatotoxic effects but unlike the mice the colon tumors arising in these animals bled frequently and could result in profound anemia."}
{"id": "PMID:1085171", "title": "A new and simple method of quantifying the bleeding time.", "content": "Disorders which produce an increase in blood loss after skin trauma do not necessarily result in a prolongation of the bleeding time. A standardized method to quantify this blood loss using the Coulter Counter model S (or other available haemoglobinometer) is reported. The need for and use of such a technique is illustrated by the investigation of primary or secondary haemostatic disorders and assessment of transfusion studies.", "contents": "A new and simple method of quantifying the bleeding time. Disorders which produce an increase in blood loss after skin trauma do not necessarily result in a prolongation of the bleeding time. A standardized method to quantify this blood loss using the Coulter Counter model S (or other available haemoglobinometer) is reported. The need for and use of such a technique is illustrated by the investigation of primary or secondary haemostatic disorders and assessment of transfusion studies."}
{"id": "PMID:1085172", "title": "Short induction treatment in acute granulocytic leukemia.", "content": "Short treatment with four cytostatics in acute granulocytic leukemia induced aplasia and reduction of total leukemic cells in 50 over 59 patients. Complete remission occured in 30 and 20 died with infectious complications during induction. Short induction treatment allowed a reduction of induction period and so a reduction of high risk period of induction before completion of complete remission.", "contents": "Short induction treatment in acute granulocytic leukemia. Short treatment with four cytostatics in acute granulocytic leukemia induced aplasia and reduction of total leukemic cells in 50 over 59 patients. Complete remission occured in 30 and 20 died with infectious complications during induction. Short induction treatment allowed a reduction of induction period and so a reduction of high risk period of induction before completion of complete remission."}
{"id": "PMID:1085174", "title": "Disorganized meridional rows in the lens epithelium of hypophysectomized frogs (Rana pipiens).", "content": "Hypophysectomy of the frog Rana pipiens causes the disappearance of mitosis in the lens epithelium. The lens epithelium from animals hypophysectomized for ten months has been found to display a disorganization of the meridional rows. Experiments with mechanical and chemical injuries suggest that cell density may be involved in column disorganization.", "contents": "Disorganized meridional rows in the lens epithelium of hypophysectomized frogs (Rana pipiens). Hypophysectomy of the frog Rana pipiens causes the disappearance of mitosis in the lens epithelium. The lens epithelium from animals hypophysectomized for ten months has been found to display a disorganization of the meridional rows. Experiments with mechanical and chemical injuries suggest that cell density may be involved in column disorganization."}
{"id": "PMID:1085175", "title": "[Blood flow distribution in the branches of frog mesentery arterial microvessels].", "content": "The blood flow distribution in 49 arterial branchings of the mesentery (R. temporaria) was investigated (D of the trunk = 25.7 + 0.0 mum). Linear rate was measured by the impulse digital chronometry of the intervals of the erythrocyte transit time. The geometric characteristics of the branching was determined in vivo, on photographs. An asymmetric structure of the investigated branching was shown; branch 1 had the inner initial cross-section which was 2.2 times greater than that of branch 2 and lesser turning angles (29 and 59 degrees). The blood flow in branch 1 was three times greater than the blood flow in branch 2; this was due to its greater inner initial cross-section and a higher linear rate. According to calculations, the blood flow resistance of the branch-turn was insignificant in the general blood flow resistance of branches; therefore the turning angle of the branches could not serve as an important regulator of the volume of the blood flowing in them. An experimentally revealed association between the blood flow in the branches, their radius and their turning angles is well described by equations of the \"optimal\" model of the vessel branching.", "contents": "[Blood flow distribution in the branches of frog mesentery arterial microvessels]. The blood flow distribution in 49 arterial branchings of the mesentery (R. temporaria) was investigated (D of the trunk = 25.7 + 0.0 mum). Linear rate was measured by the impulse digital chronometry of the intervals of the erythrocyte transit time. The geometric characteristics of the branching was determined in vivo, on photographs. An asymmetric structure of the investigated branching was shown; branch 1 had the inner initial cross-section which was 2.2 times greater than that of branch 2 and lesser turning angles (29 and 59 degrees). The blood flow in branch 1 was three times greater than the blood flow in branch 2; this was due to its greater inner initial cross-section and a higher linear rate. According to calculations, the blood flow resistance of the branch-turn was insignificant in the general blood flow resistance of branches; therefore the turning angle of the branches could not serve as an important regulator of the volume of the blood flowing in them. An experimentally revealed association between the blood flow in the branches, their radius and their turning angles is well described by equations of the \"optimal\" model of the vessel branching."}
{"id": "PMID:1085176", "title": "[Relationship between T- and B-lymphocyte cooperation and their syngeneity].", "content": "Cooperation efficiency of (CBA x C57BL/6) F1 thymocytes and CBA bone marrow cells in immune response to SRBC was compared with the syngenic combination of the same cells. Selectivity of interaction of the T- and B-lymphocytes of different origin was studied in incomplete cyclophosphamines (CBA x C57BL/6) leads to CBA chimerae, where donors were primed with SRBC and the recipients were either intact or tolerant to the given antigen. F1 T-cells proved to interact with the CAB-B-cells 10-15 times less effectively than with the syngenic B-cells. It is suggested that similarity between the antigenic structure of the cell membrane of the T- and B-lymphocytes, aiding their physical contact, increased the action efficiency of the T-mediator on the B-cell.", "contents": "[Relationship between T- and B-lymphocyte cooperation and their syngeneity]. Cooperation efficiency of (CBA x C57BL/6) F1 thymocytes and CBA bone marrow cells in immune response to SRBC was compared with the syngenic combination of the same cells. Selectivity of interaction of the T- and B-lymphocytes of different origin was studied in incomplete cyclophosphamines (CBA x C57BL/6) leads to CBA chimerae, where donors were primed with SRBC and the recipients were either intact or tolerant to the given antigen. F1 T-cells proved to interact with the CAB-B-cells 10-15 times less effectively than with the syngenic B-cells. It is suggested that similarity between the antigenic structure of the cell membrane of the T- and B-lymphocytes, aiding their physical contact, increased the action efficiency of the T-mediator on the B-cell."}
{"id": "PMID:1085177", "title": "[Use of peritoneal macrophages labeled with Cr51 and cultivated in vitro as target cells for quantitative assessment of the cytotoxic activity of immune T-lymphocytes].", "content": "Peritoneal macrophages previously labeled in a suspension with 51Cr and grown in the form of a discontinuous monolayer on glass, pretreated with poly-l-lysin were used as target cells. This permitted to estimate the cytotoxic activity of immune lymphocytes by 51Cr release for the period of 4 and 20 hours of incubation with the target. 51Cr release from such a target incubated with normal lymphocytes for 20 hours did not exceed 10-20% of the maximum release. Application of a 2% solution of sodium dodecylsulfate ensured a 100 per cent solubilization of labeled macrophages growing on the glass surface, and permitted the cytotoxic effect to be determined by measuring the label remaining in the intact cells.", "contents": "[Use of peritoneal macrophages labeled with Cr51 and cultivated in vitro as target cells for quantitative assessment of the cytotoxic activity of immune T-lymphocytes]. Peritoneal macrophages previously labeled in a suspension with 51Cr and grown in the form of a discontinuous monolayer on glass, pretreated with poly-l-lysin were used as target cells. This permitted to estimate the cytotoxic activity of immune lymphocytes by 51Cr release for the period of 4 and 20 hours of incubation with the target. 51Cr release from such a target incubated with normal lymphocytes for 20 hours did not exceed 10-20% of the maximum release. Application of a 2% solution of sodium dodecylsulfate ensured a 100 per cent solubilization of labeled macrophages growing on the glass surface, and permitted the cytotoxic effect to be determined by measuring the label remaining in the intact cells."}
{"id": "PMID:1085178", "title": "[A peculiarity in the humoral immunity of BALB/C mice with Rauscher leukemia].", "content": "There proved to be an increase in B-lymphocyte count (associated with increased synthesis of nonspecific immunoglobulins) in BALB/C mice highly sensitive to Rausher virus, after the administration of a relatively low dose of the virus. After the administration of the virus in combination with complete Freund's adjuvant in the spleen of mice, at the early periods of development of the disease, there were present normal cells synthesizing antibodies against the surface antigen of Rausher leukemia and leukemic cells with blocking antibodies adsorbed on their surface.", "contents": "[A peculiarity in the humoral immunity of BALB/C mice with Rauscher leukemia]. There proved to be an increase in B-lymphocyte count (associated with increased synthesis of nonspecific immunoglobulins) in BALB/C mice highly sensitive to Rausher virus, after the administration of a relatively low dose of the virus. After the administration of the virus in combination with complete Freund's adjuvant in the spleen of mice, at the early periods of development of the disease, there were present normal cells synthesizing antibodies against the surface antigen of Rausher leukemia and leukemic cells with blocking antibodies adsorbed on their surface."}
{"id": "PMID:1085179", "title": "[Effect of trasyslol on the permeability of the histo-hematic barrier in ischemic myocardium].", "content": "Ligation of the descending branch of the left coronary artery caused an increase in the permeability of the histo-hematic barrier of the myocardium for sulfacyl-sodium in the ischemic area and in the region adjacent to it. Trasylol proved to decrease the permeability of the histo-hematic barrier of the myocardium in the mentioned area, without influencing the intact zone. Fluoresceine test demonstrated the presence of membranotropic effect of trasylol; the preparation had no effect on the osmotic stability of erythrocytes in vitro.", "contents": "[Effect of trasyslol on the permeability of the histo-hematic barrier in ischemic myocardium]. Ligation of the descending branch of the left coronary artery caused an increase in the permeability of the histo-hematic barrier of the myocardium for sulfacyl-sodium in the ischemic area and in the region adjacent to it. Trasylol proved to decrease the permeability of the histo-hematic barrier of the myocardium in the mentioned area, without influencing the intact zone. Fluoresceine test demonstrated the presence of membranotropic effect of trasylol; the preparation had no effect on the osmotic stability of erythrocytes in vitro."}
{"id": "PMID:1085180", "title": "[Aggregated immunoglobulins as antigen-binding receptors of immune lymphocytes].", "content": "At the peak of the primary immune response to sheep erythrocytes there appeared in the spleen of mice rosette-forming cells (RFC) effectively inactivated with antibodies against aggregated mouse immunoglobulins and with the complex of polyadenylic-polyuridylic acids (poly-A, poly-U, respectively). These cells disappeared from the spleen on the 9th day after the primary immunization and were not revealed at the peak of the secondary immune response. When small splenic lymphocytes obtained on the 5th day after the immunization with sheep erythrocytes were incubated in vitro for 24 hours the total amount of the RFC inactivated by antibodies to the aggregated mouse immunoglobulin disappeared completely. These data can be considered as an indication of the existence at the peak of the primary immune response of rosette-forming cells having the antigen-antibody complexes in the capacity of the antigen-binding receptors.", "contents": "[Aggregated immunoglobulins as antigen-binding receptors of immune lymphocytes]. At the peak of the primary immune response to sheep erythrocytes there appeared in the spleen of mice rosette-forming cells (RFC) effectively inactivated with antibodies against aggregated mouse immunoglobulins and with the complex of polyadenylic-polyuridylic acids (poly-A, poly-U, respectively). These cells disappeared from the spleen on the 9th day after the primary immunization and were not revealed at the peak of the secondary immune response. When small splenic lymphocytes obtained on the 5th day after the immunization with sheep erythrocytes were incubated in vitro for 24 hours the total amount of the RFC inactivated by antibodies to the aggregated mouse immunoglobulin disappeared completely. These data can be considered as an indication of the existence at the peak of the primary immune response of rosette-forming cells having the antigen-antibody complexes in the capacity of the antigen-binding receptors."}
{"id": "PMID:1085181", "title": "[Cytotoxic and complement-binding activity of rabbit antisera against the cortex and cerebral white matter of rats and humans].", "content": "The cytotoxicity and complement-fixation activity of rabbit antisera against rat and human brain cortex and white matter was tested against mouse and rat thymocytes and bone marrow cells. The cytotoxic test proved to be more sensitive and accurate. The cytotoxins to rodent thymocytes were found in the antisera against human brain cortex only. At the same time cytotoxic antibodies were revealed both in the antisera rat brain cortex and white matter; but the former contained much more cytotoxic antibodies than the letter. After absorption with the same antigen the antisera against rat brain cortex lost their cytotoxic effect, but retained it in case of absorption with the white matter.", "contents": "[Cytotoxic and complement-binding activity of rabbit antisera against the cortex and cerebral white matter of rats and humans]. The cytotoxicity and complement-fixation activity of rabbit antisera against rat and human brain cortex and white matter was tested against mouse and rat thymocytes and bone marrow cells. The cytotoxic test proved to be more sensitive and accurate. The cytotoxins to rodent thymocytes were found in the antisera against human brain cortex only. At the same time cytotoxic antibodies were revealed both in the antisera rat brain cortex and white matter; but the former contained much more cytotoxic antibodies than the letter. After absorption with the same antigen the antisera against rat brain cortex lost their cytotoxic effect, but retained it in case of absorption with the white matter."}
{"id": "PMID:1085182", "title": "[Selective concurrent suppression of the process of rosette-forming cell accumulation during the immune response].", "content": "Repeated injections to mice of normal rabbit immunoglobulins preceding immunization with sheep erythrocytes inhibited the accumulation of rosette-forming cells (RFC) in the spleen, without influencing the proliferation of the antibody-forming cells and hemaggutinin production. Reduction of the RFC under these conditions occurred on account of B-cells whose antigen-binding receptors could be blocked by antibodies against the aggregated mouse immunoglobulins and a complex of polyadenylic-polyuridylic acids. Repeated injections of the competitive antigen enhanced the formation of the immunological memory to the second antigen. The problem of the origin of the immune rosette-forming B-cells and their influence on the formation on the immunological memory is discussed.", "contents": "[Selective concurrent suppression of the process of rosette-forming cell accumulation during the immune response]. Repeated injections to mice of normal rabbit immunoglobulins preceding immunization with sheep erythrocytes inhibited the accumulation of rosette-forming cells (RFC) in the spleen, without influencing the proliferation of the antibody-forming cells and hemaggutinin production. Reduction of the RFC under these conditions occurred on account of B-cells whose antigen-binding receptors could be blocked by antibodies against the aggregated mouse immunoglobulins and a complex of polyadenylic-polyuridylic acids. Repeated injections of the competitive antigen enhanced the formation of the immunological memory to the second antigen. The problem of the origin of the immune rosette-forming B-cells and their influence on the formation on the immunological memory is discussed."}
{"id": "PMID:1085183", "title": "[NAG infection of grass frog (Rana temporaria) tadpoles].", "content": "Rana temporaria tadpoles (2250) were contaminated with three types of NAG vibrio cultures. Clinical, bacteriological and morphological examinations showed the larvae to be suffering from an acute infection during the first 2 days after the contamination. Then the vibrios persisted in the tadpole organism for a long time and were excreted in the feces.", "contents": "[NAG infection of grass frog (Rana temporaria) tadpoles]. Rana temporaria tadpoles (2250) were contaminated with three types of NAG vibrio cultures. Clinical, bacteriological and morphological examinations showed the larvae to be suffering from an acute infection during the first 2 days after the contamination. Then the vibrios persisted in the tadpole organism for a long time and were excreted in the feces."}
{"id": "PMID:1085184", "title": "[T- and B-lymphocyte content in the peripheral blood of healthy subjects].", "content": "It was revealed by the method of rosette-formation that the content T- and B-lymphocytes differed in human blood depending on the time of the day and the season. T-cells were at their maximum in the morning and B-cells -- in the evening. The percentage content of B-cells was greater in autumn than in winter.", "contents": "[T- and B-lymphocyte content in the peripheral blood of healthy subjects]. It was revealed by the method of rosette-formation that the content T- and B-lymphocytes differed in human blood depending on the time of the day and the season. T-cells were at their maximum in the morning and B-cells -- in the evening. The percentage content of B-cells was greater in autumn than in winter."}
{"id": "PMID:1085185", "title": "[Cytogenetic effects of chloroquine in a culture of human lymphocytes].", "content": "Chloroquine added to human lymphocyte culture at the G1 stage had no influcence on the chromosome aberration level in the concentration of 15 mug/ml and suppressed the mitotic activity of the cells almost completely in the concentration of 60 and 100 mug/ml. At the G2 stage chloroquine in the concentration of 15 mug/ml had no cytogenetic effect and in the concentration of 100 mug/ml -- it increased the number of chromosome aberrations significantly.", "contents": "[Cytogenetic effects of chloroquine in a culture of human lymphocytes]. Chloroquine added to human lymphocyte culture at the G1 stage had no influcence on the chromosome aberration level in the concentration of 15 mug/ml and suppressed the mitotic activity of the cells almost completely in the concentration of 60 and 100 mug/ml. At the G2 stage chloroquine in the concentration of 15 mug/ml had no cytogenetic effect and in the concentration of 100 mug/ml -- it increased the number of chromosome aberrations significantly."}
{"id": "PMID:1085186", "title": "Antibody-induced von Willebrand's disease: a newly defined inhibitor syndrome.", "content": "A patient is described with clinical and laboratory evidence of von Willebrand's disease (VWD) in association with lymphosarcoma. He consistently had a bleeding time of over 20 min; factor VIIAHF, VIIAGN, and VIIVWF under 20%; and severe, diffuse hemorrhage, Following transfusion with cryoprecipitate, the patient had the expected rise in VIIAGN and VIIAHF. The patient's plasma contained an inhibitor which prevented aggregation of normal platelets by ristocetin, but which did not interfere with the measurement of VIIAGN or inactivate VIIAHF activity. The inhibitor was present in serum heated at 56 degrees C for 30 min, was present in a purified IgG fraction of serum, and was precipitated by anti-human IgG. The antibody did not interact directly with washed platelets, but appeared to interact with that portion of the factor VII protein that supports ristocetin aggregation (VIIIVWF). The data provide the first explanation for the pathophysiology of the acquired von Willebrand's syndrome.", "contents": "Antibody-induced von Willebrand's disease: a newly defined inhibitor syndrome. A patient is described with clinical and laboratory evidence of von Willebrand's disease (VWD) in association with lymphosarcoma. He consistently had a bleeding time of over 20 min; factor VIIAHF, VIIAGN, and VIIVWF under 20%; and severe, diffuse hemorrhage, Following transfusion with cryoprecipitate, the patient had the expected rise in VIIAGN and VIIAHF. The patient's plasma contained an inhibitor which prevented aggregation of normal platelets by ristocetin, but which did not interfere with the measurement of VIIAGN or inactivate VIIAHF activity. The inhibitor was present in serum heated at 56 degrees C for 30 min, was present in a purified IgG fraction of serum, and was precipitated by anti-human IgG. The antibody did not interact directly with washed platelets, but appeared to interact with that portion of the factor VII protein that supports ristocetin aggregation (VIIIVWF). The data provide the first explanation for the pathophysiology of the acquired von Willebrand's syndrome."}
{"id": "PMID:1085193", "title": "Cell-mediated immunity in patients with carcinoma: correlation between clinical stage and immunocompetence.", "content": "Cell-mediated immunity (CMI) was evaluated in 82 patients with non-lymphoid tumors by in vivo and in vitro methods. These included skin test with ubiquitous antigens, 2,4 dinitrochlorobenzene (DNCB) sensitization, determination of T and B peripheral blood lymphocytes, and lymphocyte response to phytohemagglutinin (PHA). The patients were divided into two groups: those with localized and those with disseminated disease (LD and DD). The patients with LD showed no significant differences in CMI when compared with normal controls. The patients with DD showed various defects in CMI when compared with controls and patients with LD. There were significant differences in the response to DNCB, and streptokinase-streptodornase (SK-SD) was the most discriminative of the skin-test antigens. The response to PHA was greatly depressed in patients with DD, whether in the presence of autologous or homologous plasma; in some patients a factor inhibiting to blastogenesis was detected in the serum. In patients with DD, a T-cell lymphopenia was observed. These data showed a correlation between immunocompetence and clinical stage.", "contents": "Cell-mediated immunity in patients with carcinoma: correlation between clinical stage and immunocompetence. Cell-mediated immunity (CMI) was evaluated in 82 patients with non-lymphoid tumors by in vivo and in vitro methods. These included skin test with ubiquitous antigens, 2,4 dinitrochlorobenzene (DNCB) sensitization, determination of T and B peripheral blood lymphocytes, and lymphocyte response to phytohemagglutinin (PHA). The patients were divided into two groups: those with localized and those with disseminated disease (LD and DD). The patients with LD showed no significant differences in CMI when compared with normal controls. The patients with DD showed various defects in CMI when compared with controls and patients with LD. There were significant differences in the response to DNCB, and streptokinase-streptodornase (SK-SD) was the most discriminative of the skin-test antigens. The response to PHA was greatly depressed in patients with DD, whether in the presence of autologous or homologous plasma; in some patients a factor inhibiting to blastogenesis was detected in the serum. In patients with DD, a T-cell lymphopenia was observed. These data showed a correlation between immunocompetence and clinical stage."}
{"id": "PMID:1085194", "title": "Correlation of regional lymph node in vitro antitumor immunoreactivity histology with colorectal carcinoma.", "content": "Lymph nodes from resected specimens of human colorectal carcinoma were investigated for in vitro lymphocyte cytotoxicity against primary cultures of autologous tumor cells. Regional lymph node lymphocytes were cytotoxic in 32 of 142 cases (23%). Altogether 200 nodes were examined and the cytotoxicity correlated directly with sinus histiocytosis, seen in 43 nodes from 35 cases, and with hyperplasia of B- and T-lymphocyte areas combined, seen in 92 nodes from 65 cases. Lymph nodes with combined B- and T-cell hyperplasia were significantly more common in cases of good tumor differentiation. The findings suggest that sinus histiocytosis and hyperplasia of both major lymphocyte populations are morphological expressions of in vitro antitumor immunoreactivity in the regional lymph node.", "contents": "Correlation of regional lymph node in vitro antitumor immunoreactivity histology with colorectal carcinoma. Lymph nodes from resected specimens of human colorectal carcinoma were investigated for in vitro lymphocyte cytotoxicity against primary cultures of autologous tumor cells. Regional lymph node lymphocytes were cytotoxic in 32 of 142 cases (23%). Altogether 200 nodes were examined and the cytotoxicity correlated directly with sinus histiocytosis, seen in 43 nodes from 35 cases, and with hyperplasia of B- and T-lymphocyte areas combined, seen in 92 nodes from 65 cases. Lymph nodes with combined B- and T-cell hyperplasia were significantly more common in cases of good tumor differentiation. The findings suggest that sinus histiocytosis and hyperplasia of both major lymphocyte populations are morphological expressions of in vitro antitumor immunoreactivity in the regional lymph node."}
{"id": "PMID:1085195", "title": "High terminal deoxynucleotidyl transferase activity in acute myelogenous leukemia.", "content": "Although leukocytes from all 13 acute lymphoblastic leukemia patients examined had high terminal deoxynucleotidyl transferase (terminal transferase) activity (20 to 100 units/mg of cellular DNA, where 1 unit equals 1 nmole of nucleotide polymerized in 1 hr) and those from 21 acute myelocytic leukemia patients had low terminal transferase activity (0.2 to 2 units/mg of cellular DNA), the bone marrow and peripheral blood leukocytes from 2 patients with acute myelocytic leukemia, diagnosed on the basis of clinical features and the morphology, cytochemistry, and cytogenetics of the leukemic cells, had terminal transferase activity (39 to 52 units/mg of cellular DNA) equivalent to that found in leukemic lymphoblasts. These results bring under question the specificity of high terminal transferase activity outside of the thymus as a marker for leukemic lymphoblasts and, secondarily, the derivation of acute lymphoblastic leukemia cells in all cases from thymocytes. Perhaps malignant transformation in a pleuripotent stem cell with derepression of the genome for terminal transferase could account for high terminal transferase activity observed in certain leukemic cells.", "contents": "High terminal deoxynucleotidyl transferase activity in acute myelogenous leukemia. Although leukocytes from all 13 acute lymphoblastic leukemia patients examined had high terminal deoxynucleotidyl transferase (terminal transferase) activity (20 to 100 units/mg of cellular DNA, where 1 unit equals 1 nmole of nucleotide polymerized in 1 hr) and those from 21 acute myelocytic leukemia patients had low terminal transferase activity (0.2 to 2 units/mg of cellular DNA), the bone marrow and peripheral blood leukocytes from 2 patients with acute myelocytic leukemia, diagnosed on the basis of clinical features and the morphology, cytochemistry, and cytogenetics of the leukemic cells, had terminal transferase activity (39 to 52 units/mg of cellular DNA) equivalent to that found in leukemic lymphoblasts. These results bring under question the specificity of high terminal transferase activity outside of the thymus as a marker for leukemic lymphoblasts and, secondarily, the derivation of acute lymphoblastic leukemia cells in all cases from thymocytes. Perhaps malignant transformation in a pleuripotent stem cell with derepression of the genome for terminal transferase could account for high terminal transferase activity observed in certain leukemic cells."}
{"id": "PMID:1085198", "title": "Continuous culturing of murine splenic B-lumphocytes: synthesis and surface deposition of IgM and putative IgD molecules.", "content": "A method for the continuous culturing of mouse splenic lymphocytes is described. The method utilizes rat-tail collagen-coated glass or plastic surfaces which enhance the adherence and proliferation of B-lymphocytes. The cultured cells are shown to synthesize IgM and putative IgD molecules which are deposited on the cell membrane. The majority of the cells possessed Fc receptors and C' receptors, while none of them seemed to possess theta-antigen. It is concluded that these cells are functionally active B-lymphoid cells.", "contents": "Continuous culturing of murine splenic B-lumphocytes: synthesis and surface deposition of IgM and putative IgD molecules. A method for the continuous culturing of mouse splenic lymphocytes is described. The method utilizes rat-tail collagen-coated glass or plastic surfaces which enhance the adherence and proliferation of B-lymphocytes. The cultured cells are shown to synthesize IgM and putative IgD molecules which are deposited on the cell membrane. The majority of the cells possessed Fc receptors and C' receptors, while none of them seemed to possess theta-antigen. It is concluded that these cells are functionally active B-lymphoid cells."}
{"id": "PMID:1085204", "title": "Arenaviruses: purification and physicochemical nature.", "content": "The arenaviruses are a group of enveloped viruses having as a unique morphological finding the presence in the virion of granules instead of a defined core. The viruses contain a single-stranded RNA genome, but appreciable amounts of ribosomal-like RNA and 4-6S RNA of host cell origin have been detected. Little information is available on the mode of replication of the viral nucleic acids. A virion-associated RNA-dependent RNA polymerase has been described and there is indirect evidence to suggest that host cell RNA or DNA participates in virus replication. However, the steps in viral RNA synthesis and expression have not yet been elucidated.Pichinde virus contains 2 glycoproteins and 2 polypeptides. Cells infected with Pichinde virus or LCM virus have been shown to produce 2 antigens detectable by immunodiffusion. Both antigens appear to be components of the virion, but the relation between the antigens detected by immunodiffusion and the polypeptides detected by polyacrylamide gel electrophoresis has not yet been clarified.", "contents": "Arenaviruses: purification and physicochemical nature. The arenaviruses are a group of enveloped viruses having as a unique morphological finding the presence in the virion of granules instead of a defined core. The viruses contain a single-stranded RNA genome, but appreciable amounts of ribosomal-like RNA and 4-6S RNA of host cell origin have been detected. Little information is available on the mode of replication of the viral nucleic acids. A virion-associated RNA-dependent RNA polymerase has been described and there is indirect evidence to suggest that host cell RNA or DNA participates in virus replication. However, the steps in viral RNA synthesis and expression have not yet been elucidated.Pichinde virus contains 2 glycoproteins and 2 polypeptides. Cells infected with Pichinde virus or LCM virus have been shown to produce 2 antigens detectable by immunodiffusion. Both antigens appear to be components of the virion, but the relation between the antigens detected by immunodiffusion and the polypeptides detected by polyacrylamide gel electrophoresis has not yet been clarified."}
{"id": "PMID:1085205", "title": "LCM virus infection of cells in vitro.", "content": "Most mammalian cells cultivated in vitro can be infected with lymphocytic choriomeningitis (LCM) virus. In addition to infectious virus, the cells produce antigenic material that fixes complement in the presence of antibody and is precipitated by antiserum. Intracellular antigen can also be demonstrated by the immunofluorescence procedure. When infected cells are viewed with the electron microscope, viral structures are seen either budding from or in association with the cell membranes. Immunoelectron microscopy, immunofluorescence, and cytotoxicity tests reveal virus-specific antigens on the surface of intact cells. Virus multiplication may be succeeded by cytolysis. Two LCM virus-specific antigens (or antigenic groups) can at present be distinguished. One corresponds to the infectious virus; the other is the complement-fixing \"soluble\" antigen. This extractable complement-fixing activity is produced by infected cells and is also a structural component of the infectious virus. It is not represented on the surface of either the virion or the infected cell. The cytolytic potential of LCM virus varies and is dependent on its previous passage history. Cytolytic and \"attenuated\" variants are able to initiate persistent infection of Mus musculus.Together with infectious virus, particles are produced that temporarily protect cells against standard virus. They appear to be by-products of virus multiplication, not in the sense of deletion mutants but of virus structures insufficiently equipped for their own active or passive replication, though capable of interfering with infectious virus. No evidence has been found for the generation of \"defective interfering\" particles, though their presence has not yet been excluded.", "contents": "LCM virus infection of cells in vitro. Most mammalian cells cultivated in vitro can be infected with lymphocytic choriomeningitis (LCM) virus. In addition to infectious virus, the cells produce antigenic material that fixes complement in the presence of antibody and is precipitated by antiserum. Intracellular antigen can also be demonstrated by the immunofluorescence procedure. When infected cells are viewed with the electron microscope, viral structures are seen either budding from or in association with the cell membranes. Immunoelectron microscopy, immunofluorescence, and cytotoxicity tests reveal virus-specific antigens on the surface of intact cells. Virus multiplication may be succeeded by cytolysis. Two LCM virus-specific antigens (or antigenic groups) can at present be distinguished. One corresponds to the infectious virus; the other is the complement-fixing \"soluble\" antigen. This extractable complement-fixing activity is produced by infected cells and is also a structural component of the infectious virus. It is not represented on the surface of either the virion or the infected cell. The cytolytic potential of LCM virus varies and is dependent on its previous passage history. Cytolytic and \"attenuated\" variants are able to initiate persistent infection of Mus musculus.Together with infectious virus, particles are produced that temporarily protect cells against standard virus. They appear to be by-products of virus multiplication, not in the sense of deletion mutants but of virus structures insufficiently equipped for their own active or passive replication, though capable of interfering with infectious virus. No evidence has been found for the generation of \"defective interfering\" particles, though their presence has not yet been excluded."}
{"id": "PMID:1085206", "title": "Immune responses to LCM virus infection in vivo and in vitro. Mechanisms of immune-mediated disease.", "content": "Both recovery and death of mice following acute infections with lymphocytic choriomeningitis virus appear to be mediated by a population of virus-specific thymus-derived (T) effector lymphocytes that possess lytic activity in vitro against virus-infected syngeneic fibroblasts. Whether recovery or death occurs is determined by the balance between two interdependent factors: (1) the extent of virus-induced modifications in the surfaces of cells comprising \"target\" tissues, and (2) the efficiency of the immune inductive process leading to the generation of effector T lymphocytes that recognize and destroy these modified cells.", "contents": "Immune responses to LCM virus infection in vivo and in vitro. Mechanisms of immune-mediated disease. Both recovery and death of mice following acute infections with lymphocytic choriomeningitis virus appear to be mediated by a population of virus-specific thymus-derived (T) effector lymphocytes that possess lytic activity in vitro against virus-infected syngeneic fibroblasts. Whether recovery or death occurs is determined by the balance between two interdependent factors: (1) the extent of virus-induced modifications in the surfaces of cells comprising \"target\" tissues, and (2) the efficiency of the immune inductive process leading to the generation of effector T lymphocytes that recognize and destroy these modified cells."}
{"id": "PMID:1085207", "title": "Persistent LCM virus infection in the mouse. Immunity and tolerance.", "content": "The initiation of persistent infections with the Traub strain of the LCM virus is dependent not only on the number of immunocompetent cells present in the infected animals but probably also on the fact that the virus depresses the development of the bone marrow cells and causes a pronounced immunosuppression at the T cell level. By analysing the events leading to the termination of the virus carrier state by adoptive immunization, it was clearly demonstrated that the cellular immunity provoked was solely responsible for the virus elimination. Furthermore, helper T cells were shown to be necessary for the production of antibodies, which also occurs in adoptively immunized mice. In view of this finding, further experiments were performed, which strongly indicated that neither LCM-primed B cells nor LCM-primed helper T cells are present in mice that are persistent virus carriers. Similarly, neither cellular immunity to the LCM virus nor the presence of enhancing factors or suppressor cells could be detected. It is concluded that C(3)H mice that are persistent virus carriers have developed a humoral as well as a cellular immunological tolerance to the LCM virus.", "contents": "Persistent LCM virus infection in the mouse. Immunity and tolerance. The initiation of persistent infections with the Traub strain of the LCM virus is dependent not only on the number of immunocompetent cells present in the infected animals but probably also on the fact that the virus depresses the development of the bone marrow cells and causes a pronounced immunosuppression at the T cell level. By analysing the events leading to the termination of the virus carrier state by adoptive immunization, it was clearly demonstrated that the cellular immunity provoked was solely responsible for the virus elimination. Furthermore, helper T cells were shown to be necessary for the production of antibodies, which also occurs in adoptively immunized mice. In view of this finding, further experiments were performed, which strongly indicated that neither LCM-primed B cells nor LCM-primed helper T cells are present in mice that are persistent virus carriers. Similarly, neither cellular immunity to the LCM virus nor the presence of enhancing factors or suppressor cells could be detected. It is concluded that C(3)H mice that are persistent virus carriers have developed a humoral as well as a cellular immunological tolerance to the LCM virus."}
{"id": "PMID:1085208", "title": "Neurobiology of LCM virus infection in rodents.", "content": "In addition to the previously reported data on the retinal and cerebellar immunopathology following infection of neonatal rats with LCM virus, we have found that there are long-term effects on behavioural and neurological development. Rats were inoculated intracerebrally with the E-350 strain at different ages during the first 3 weeks after birth. Behavioural tests were initiated when the animals were either 3 months or 1 year of age. The behavioural consequences appear to be a long-term alteration in emotional reactivity such that the infected animals are less responsive than controls as assessed by these measures. No alterations were detected in animals infected after the first postnatal week. Complementing these data are the findings of a collaterally progressive lesion of the hippocampal dentate gyrus as well as a loss in total cell number in the forebrain (as assessed by DNA, RNA, and protein determinations) amounting to about 20% of the brain mass. These behavioural, histological, and biochemical data indicate that there are forebrain structures, most probably within the limbic system, that are susceptible at critical phases of development to the pathological consequences of infection with LCM virus.", "contents": "Neurobiology of LCM virus infection in rodents. In addition to the previously reported data on the retinal and cerebellar immunopathology following infection of neonatal rats with LCM virus, we have found that there are long-term effects on behavioural and neurological development. Rats were inoculated intracerebrally with the E-350 strain at different ages during the first 3 weeks after birth. Behavioural tests were initiated when the animals were either 3 months or 1 year of age. The behavioural consequences appear to be a long-term alteration in emotional reactivity such that the infected animals are less responsive than controls as assessed by these measures. No alterations were detected in animals infected after the first postnatal week. Complementing these data are the findings of a collaterally progressive lesion of the hippocampal dentate gyrus as well as a loss in total cell number in the forebrain (as assessed by DNA, RNA, and protein determinations) amounting to about 20% of the brain mass. These behavioural, histological, and biochemical data indicate that there are forebrain structures, most probably within the limbic system, that are susceptible at critical phases of development to the pathological consequences of infection with LCM virus."}
{"id": "PMID:1085209", "title": "The pathology of human Lassa fever.", "content": "Pathological findings have been described in only a small number of cases of Lassa fever since the virus was first isolated in 1969. Morphologically, eosinophilic necrosis of hepatocytes was the most frequent finding and focal necroses, often extensive, were present in most cases. These findings are similar to the lesions previously described in Argentinian and Bolivian haemorrhagic fever. Focal interstitial pneumonitis, focal tubular necrosis in the kidney, lymphocytic infiltration of the splenic veins, and partial replacement of the splenic follicles by amorphous eosinophilic material have been described, but the significance of these findings is unclear. More detailed and sophisticated investigations are required in the future if pathogenetic mechanisms are to be unravelled.", "contents": "The pathology of human Lassa fever. Pathological findings have been described in only a small number of cases of Lassa fever since the virus was first isolated in 1969. Morphologically, eosinophilic necrosis of hepatocytes was the most frequent finding and focal necroses, often extensive, were present in most cases. These findings are similar to the lesions previously described in Argentinian and Bolivian haemorrhagic fever. Focal interstitial pneumonitis, focal tubular necrosis in the kidney, lymphocytic infiltration of the splenic veins, and partial replacement of the splenic follicles by amorphous eosinophilic material have been described, but the significance of these findings is unclear. More detailed and sophisticated investigations are required in the future if pathogenetic mechanisms are to be unravelled."}
{"id": "PMID:1085210", "title": "Recent outbreaks of lymphocytic choriomeningitis in the United States of America.", "content": "Lymphocytic choriomeningitis (LCM) has been rarely reported in the American literature since 1960. It is interesting that each of the 3 epidemics reported since then has been associated with exposure to hamsters. In 1973, 48 cases of LCM spanning the years 1971-1973 occurred at the University of Rochester Medical School associated with hamsters implanted with tumour tissues. These tissues were found to be LCM-positive, as in an earlier outbreak in 1965 at the National Institutes of Health. A nationwide outbreak of LCM occurred in late 1973 and early 1974 totalling at least 181 cases in 12 states; all were associated with pet hamsters from a single breeder in Birmingham, Alabama. He was an employee of a biological products firm whose tumour tissues were found positive for LCM and were also incriminated in the 1973 Rochester outbreak. The last outbreak occurred in a graduate school laboratory in New York State involving 7 individuals working with hamster tumours from the same Birmingham biological firm. The nationwide epidemic ended in middle April 1974 following removal of incriminated hamsters from pet shops throughout the country and voluntary cessation of distribution of hamsters from the incriminated breeder. The biological firm notified all laboratories of the possible contamination of tumours and has voluntarily stopped distribution of known positive tumours.", "contents": "Recent outbreaks of lymphocytic choriomeningitis in the United States of America. Lymphocytic choriomeningitis (LCM) has been rarely reported in the American literature since 1960. It is interesting that each of the 3 epidemics reported since then has been associated with exposure to hamsters. In 1973, 48 cases of LCM spanning the years 1971-1973 occurred at the University of Rochester Medical School associated with hamsters implanted with tumour tissues. These tissues were found to be LCM-positive, as in an earlier outbreak in 1965 at the National Institutes of Health. A nationwide outbreak of LCM occurred in late 1973 and early 1974 totalling at least 181 cases in 12 states; all were associated with pet hamsters from a single breeder in Birmingham, Alabama. He was an employee of a biological products firm whose tumour tissues were found positive for LCM and were also incriminated in the 1973 Rochester outbreak. The last outbreak occurred in a graduate school laboratory in New York State involving 7 individuals working with hamster tumours from the same Birmingham biological firm. The nationwide epidemic ended in middle April 1974 following removal of incriminated hamsters from pet shops throughout the country and voluntary cessation of distribution of hamsters from the incriminated breeder. The biological firm notified all laboratories of the possible contamination of tumours and has voluntarily stopped distribution of known positive tumours."}
{"id": "PMID:1085211", "title": "Some observations on hamster-derived human infection with lymphocytic choriomeningitis virus.", "content": "The sequence of events leading to the diagnosis of recent outbreaks of hamster-borne lymphocytic choriomeningitis (LCM) in New York State is briefly reviewed. Some relevant experiments on persistent LCM infection of hamsters are described, including measurements of complement levels in the affected animals. Evidence is discussed which suggests that complement has an important role in eliminating free virus in the circulation by participating in virus neutralization.", "contents": "Some observations on hamster-derived human infection with lymphocytic choriomeningitis virus. The sequence of events leading to the diagnosis of recent outbreaks of hamster-borne lymphocytic choriomeningitis (LCM) in New York State is briefly reviewed. Some relevant experiments on persistent LCM infection of hamsters are described, including measurements of complement levels in the affected animals. Evidence is discussed which suggests that complement has an important role in eliminating free virus in the circulation by participating in virus neutralization."}
{"id": "PMID:1085212", "title": "Clinical and epidemiological patterns of Argentine haemorrhagic fever.", "content": "The epidemiology of Argentine haemorrhagic fever (AHF) is closely related to cricetine rodents acting as natural hosts of Junin virus. The endemo-epidemic area, which has increased 5 times since the disease was first recognized 15-20 years ago, is located in a densely populated region of Argentina. It has been shown that the virus of LCM is active in humans and rodents of the AHF endemic area; this demonstrates the simultaneous presence of two arenaviruses pathogenic for man in a given geographic location.The disease is characterized by haematological, renal, neurological and cardiovascular changes. Electron microscopy and immunohistochemical studies have shown cytopathic changes, characteristic intracellular virus-like particles, and antigenic determinants of Junin virus in different organs from 9 cases of AHF. No deposits of immunoglobulins or C3 were found in the kidneys; in addition, an absence of fibrinogen and C3 in the hepatocytes and of immunoglobulins in the spleen was observed. These findings suggest a direct viral pathogenic action in the human disease.Ultrastructural and immunofluorescence studies in tissues of guinea-pigs inoculated with two strains of Junin virus revealed the presence of the same types of virus-like particles and antigenic determinants of Junin virus as were encountered in the human subjects with AHF.", "contents": "Clinical and epidemiological patterns of Argentine haemorrhagic fever. The epidemiology of Argentine haemorrhagic fever (AHF) is closely related to cricetine rodents acting as natural hosts of Junin virus. The endemo-epidemic area, which has increased 5 times since the disease was first recognized 15-20 years ago, is located in a densely populated region of Argentina. It has been shown that the virus of LCM is active in humans and rodents of the AHF endemic area; this demonstrates the simultaneous presence of two arenaviruses pathogenic for man in a given geographic location.The disease is characterized by haematological, renal, neurological and cardiovascular changes. Electron microscopy and immunohistochemical studies have shown cytopathic changes, characteristic intracellular virus-like particles, and antigenic determinants of Junin virus in different organs from 9 cases of AHF. No deposits of immunoglobulins or C3 were found in the kidneys; in addition, an absence of fibrinogen and C3 in the hepatocytes and of immunoglobulins in the spleen was observed. These findings suggest a direct viral pathogenic action in the human disease.Ultrastructural and immunofluorescence studies in tissues of guinea-pigs inoculated with two strains of Junin virus revealed the presence of the same types of virus-like particles and antigenic determinants of Junin virus as were encountered in the human subjects with AHF."}
{"id": "PMID:1085213", "title": "Surveillance of Lassa fever in missionaries stationed in West Africa.", "content": "To determine the distribution of Lassa virus in West Africa, a serological survey was undertaken. A number of mission hospital supplied sera from patients admitted with a history of fever and specimens were also collected in New York from missionaries who had experienced an unusual febrile illness while working in Africa. More cases of Lassa fever were detected among missionaries than among Africans, possibly because many African patients had left hospital before the complement fixation tests had become positive. Although most adults had fairly high fever and some were prostrated, fever was less severe in the children examined. In general the findings confirm that not all Lassa fever patients have the severe syndrome described in the original reports.", "contents": "Surveillance of Lassa fever in missionaries stationed in West Africa. To determine the distribution of Lassa virus in West Africa, a serological survey was undertaken. A number of mission hospital supplied sera from patients admitted with a history of fever and specimens were also collected in New York from missionaries who had experienced an unusual febrile illness while working in Africa. More cases of Lassa fever were detected among missionaries than among Africans, possibly because many African patients had left hospital before the complement fixation tests had become positive. Although most adults had fairly high fever and some were prostrated, fever was less severe in the children examined. In general the findings confirm that not all Lassa fever patients have the severe syndrome described in the original reports."}
{"id": "PMID:1085214", "title": "Lassa fever in Onitsha, East Central State, Nigeria in 1974.", "content": "Three cases of Lassa fever occurred in Onitsha, East Central State, Nigeria, in January and February 1974. The first case was a 19-year-old Nigerian; the other 2 cases were German missionary physicians at St Charles Borromeo Hospital, Onitsha, one of whom cared for the patient who was the first case. Thus, 2 of the 3 cases were hospital acquired. Investigations failed to discover a village outbreak or the source of virus for the first case. A serosurvey of 258 hospital staff members and contacts of the 3 cases showed no other persons with antibody to Lassa virus. The absence of Lassa virus antibody in a high-risk group indicates a low or nonexistent level of past Lassa virus activity in southeastern Nigeria.", "contents": "Lassa fever in Onitsha, East Central State, Nigeria in 1974. Three cases of Lassa fever occurred in Onitsha, East Central State, Nigeria, in January and February 1974. The first case was a 19-year-old Nigerian; the other 2 cases were German missionary physicians at St Charles Borromeo Hospital, Onitsha, one of whom cared for the patient who was the first case. Thus, 2 of the 3 cases were hospital acquired. Investigations failed to discover a village outbreak or the source of virus for the first case. A serosurvey of 258 hospital staff members and contacts of the 3 cases showed no other persons with antibody to Lassa virus. The absence of Lassa virus antibody in a high-risk group indicates a low or nonexistent level of past Lassa virus activity in southeastern Nigeria."}
{"id": "PMID:1085215", "title": "Use of the complement fixation (CF) test in Lassa fever surveillance. Evidence for persistent CF antibodies.", "content": "A survey to detect individuals with antibodies to Lassa virus was undertaken among hospital personnel in the eastern and southern provinces of Sierra Leone late in 1974. Sera were evaluated by the complement fixation test. The data obtained showed that some contacts of Lassa fever patients in the 1972 epidemic had developed antibodies to the virus; individuals who had never reported being sick also showed evidence of infection, with significant CF antibody titres in their sera. Surviving Lassa fever patients from the 1972 epidemic still had easily measurable levels of persisting CF antibodies. The significance of these data is discussed; in addition it is recommended that the CF test should continue to be the method of choice in mass surveys for this virus disease until other tests can be developed.", "contents": "Use of the complement fixation (CF) test in Lassa fever surveillance. Evidence for persistent CF antibodies. A survey to detect individuals with antibodies to Lassa virus was undertaken among hospital personnel in the eastern and southern provinces of Sierra Leone late in 1974. Sera were evaluated by the complement fixation test. The data obtained showed that some contacts of Lassa fever patients in the 1972 epidemic had developed antibodies to the virus; individuals who had never reported being sick also showed evidence of infection, with significant CF antibody titres in their sera. Surviving Lassa fever patients from the 1972 epidemic still had easily measurable levels of persisting CF antibodies. The significance of these data is discussed; in addition it is recommended that the CF test should continue to be the method of choice in mass surveys for this virus disease until other tests can be developed."}
{"id": "PMID:1085216", "title": "Recent isolations of Lassa virus from Nigerian rodents.", "content": "Rodents were trapped in the Benue-Plateau and North-Eastern States of Nigeria where Lassa fever had been reported in previous years. Eight Lassa virus strains were isolated from tissues and blood of rodents identified in the field as being of 3 different species: Mastomys natalensis, Rattus rattus, and Mus minutoides. All the infected rodents were collected in village habitats. These isolations indicate the presence of Lassa virus in wild rodents in Nigeria during periods when no human infections were evident.Prior studies in Sierra Leone have indicated that a single rodent species, M. natalensis, may be the important reservoir host of Lassa virus. Since the present study indicates that other rodent species may be involved as well, the ecology of Lassa virus may be more complicated than was heretofore supposed. In view of the importance of determining the geographic and species range of rodent hosts of Lassa virus, and because of the problems inherent in rodent identification under austere field conditions, it is urgent that further studies be conducted in the same areas of Nigeria to confirm these findings.", "contents": "Recent isolations of Lassa virus from Nigerian rodents. Rodents were trapped in the Benue-Plateau and North-Eastern States of Nigeria where Lassa fever had been reported in previous years. Eight Lassa virus strains were isolated from tissues and blood of rodents identified in the field as being of 3 different species: Mastomys natalensis, Rattus rattus, and Mus minutoides. All the infected rodents were collected in village habitats. These isolations indicate the presence of Lassa virus in wild rodents in Nigeria during periods when no human infections were evident.Prior studies in Sierra Leone have indicated that a single rodent species, M. natalensis, may be the important reservoir host of Lassa virus. Since the present study indicates that other rodent species may be involved as well, the ecology of Lassa virus may be more complicated than was heretofore supposed. In view of the importance of determining the geographic and species range of rodent hosts of Lassa virus, and because of the problems inherent in rodent identification under austere field conditions, it is urgent that further studies be conducted in the same areas of Nigeria to confirm these findings."}
{"id": "PMID:1085217", "title": "Viruses other than arenaviruses from West African wild mammals. Factors affecting transmission to man and domestic animals.", "content": "At least thirty-seven different viruses have been isolated from wild mammals in West Africa since 1962. Some of these, including Lassa virus, are already known to cause serious human morbidity and mortality. Crimean haemorrhagic fever-Congo virus, Dugbe virus, Mokola virus, and a smallpox-like agent from a gerbil in Dahomey are briefly discussed. An account of social and ecologic factors affecting man, domestic animals, and their interaction with wild mammals is given.", "contents": "Viruses other than arenaviruses from West African wild mammals. Factors affecting transmission to man and domestic animals. At least thirty-seven different viruses have been isolated from wild mammals in West Africa since 1962. Some of these, including Lassa virus, are already known to cause serious human morbidity and mortality. Crimean haemorrhagic fever-Congo virus, Dugbe virus, Mokola virus, and a smallpox-like agent from a gerbil in Dahomey are briefly discussed. An account of social and ecologic factors affecting man, domestic animals, and their interaction with wild mammals is given."}
{"id": "PMID:1085218", "title": "The ecology of Praomys (Mastomys) natalensis in southern Africa.", "content": "The only non-human host of Lassa virus so far identified is the multimammate mouse, Praomys (Mastomys) natalensis, but its precise role in the natural Lassa fever cycle remains to be determined. This species is also an important link in the plague cycle in southern Africa and is one of the commonest rodents of Africa. It is a prolific breeder and can be kept and bred easily in captivity. It is thus an excellent laboratory animal, although it needs to be handled with care because it is aggressive towards man and bites readily. The current status of knowledge of its taxonomy, ecology, distribution, and role as a disease vector is reviewed, but attention is drawn to the possibly disastrous consequences of attempting to eradicate a vector species before the natural cycle of the disease and the ecology of the vector are fully understood.", "contents": "The ecology of Praomys (Mastomys) natalensis in southern Africa. The only non-human host of Lassa virus so far identified is the multimammate mouse, Praomys (Mastomys) natalensis, but its precise role in the natural Lassa fever cycle remains to be determined. This species is also an important link in the plague cycle in southern Africa and is one of the commonest rodents of Africa. It is a prolific breeder and can be kept and bred easily in captivity. It is thus an excellent laboratory animal, although it needs to be handled with care because it is aggressive towards man and bites readily. The current status of knowledge of its taxonomy, ecology, distribution, and role as a disease vector is reviewed, but attention is drawn to the possibly disastrous consequences of attempting to eradicate a vector species before the natural cycle of the disease and the ecology of the vector are fully understood."}
{"id": "PMID:1085219", "title": "The biology, behaviour, and ecology of Mastomys natalensis in southern Africa.", "content": "The multimammate mouse, Mastomys natalensis, is the most widespread and common rodent in Africa south of the Sahara. It is an ideal carrier of normally non-human diseases to the domestic environment, not only because of its semi-commensal habit but also because of a combination of other behavioural and ecological factors. Of these, the most important is an exceptionally high propagation rate.", "contents": "The biology, behaviour, and ecology of Mastomys natalensis in southern Africa. The multimammate mouse, Mastomys natalensis, is the most widespread and common rodent in Africa south of the Sahara. It is an ideal carrier of normally non-human diseases to the domestic environment, not only because of its semi-commensal habit but also because of a combination of other behavioural and ecological factors. Of these, the most important is an exceptionally high propagation rate."}
{"id": "PMID:1085220", "title": "Spontaneous diseases in a closed colony of Praomys (Mastomys) natalensis.", "content": "In comparison with other rodents, the mastomys is unique as regards the patterns of neoplastic and non-neoplastic diseases that it develops, some of which may constitute suitable animal models for corresponding diseases of man. Among the spontaneous diseases commonly encountered in necropsies of 600 mastomys maintained in a closed colony were: (1) degenerative joint disease of diarthroses and interventricular disks, which develops regularly in the second year of life; (2) renal disease, a type of immune-complex glomerulonephritis, affecting approximately 80% of mastomys in the age bracket 18-36 months; (3) thymomas and thymic hyperplasia affecting 30% of mastomys by the time they are 2 years old or older; (4) a combination of thymoma and polymyositis in a mastomys showing serum-globulin reactivity, presumably auto-antibody against striated muscle; (5) beginning before the age of 1 year, replacement of the normal cell population of the lymphoid tissues by plasma cells, and intense plasma cell infiltration at many other organ and tissue sites; (6) haematopoietic neoplasms, without leukaemic blood, in 10% of mastomys; and (7) histamine-producing argyrophilic carcinoid tumours of the glandular stomach in approximately 60% of old male and 30% of old female mastomys. Additionally, other neoplasms not infrequently encountered in mastomys rarely occur in other rodent species; conversely some neoplasms commonly found in other rodents, including especially tumours of the lung and mammary gland and leukaemia, are rare or absent in mastomys.", "contents": "Spontaneous diseases in a closed colony of Praomys (Mastomys) natalensis. In comparison with other rodents, the mastomys is unique as regards the patterns of neoplastic and non-neoplastic diseases that it develops, some of which may constitute suitable animal models for corresponding diseases of man. Among the spontaneous diseases commonly encountered in necropsies of 600 mastomys maintained in a closed colony were: (1) degenerative joint disease of diarthroses and interventricular disks, which develops regularly in the second year of life; (2) renal disease, a type of immune-complex glomerulonephritis, affecting approximately 80% of mastomys in the age bracket 18-36 months; (3) thymomas and thymic hyperplasia affecting 30% of mastomys by the time they are 2 years old or older; (4) a combination of thymoma and polymyositis in a mastomys showing serum-globulin reactivity, presumably auto-antibody against striated muscle; (5) beginning before the age of 1 year, replacement of the normal cell population of the lymphoid tissues by plasma cells, and intense plasma cell infiltration at many other organ and tissue sites; (6) haematopoietic neoplasms, without leukaemic blood, in 10% of mastomys; and (7) histamine-producing argyrophilic carcinoid tumours of the glandular stomach in approximately 60% of old male and 30% of old female mastomys. Additionally, other neoplasms not infrequently encountered in mastomys rarely occur in other rodent species; conversely some neoplasms commonly found in other rodents, including especially tumours of the lung and mammary gland and leukaemia, are rare or absent in mastomys."}
{"id": "PMID:1085221", "title": "Lassa virus infection in Mastomys natalensis in Sierra Leone. Gross and microscopic findings in infected and uninfected animals.", "content": "Pathological examinations of 28 wild-caught Mastomys natalensis from Sierra Leone, 14 of which were positive for Lassa virus by tissue culture, are reported. The high frequency of neoplastic and degenerative diseases observed among older animals in closed colonies of M. natalensis were not observed in the wild animals studied. This is probably a reflection of the age distribution of the study population, since the life expectancy of wild Mastomys is less than a year. Inflammatory lesions were nonetheless identified, some of which were similar to those described in laboratory colonies. Frequent lesions were myocarditis (54%), myositis (32%), interstitial pneumonitis (50%), intercapillary glomerulosclerosis (36%), and acute nephrosis (14%). Follicular and nodular lymphoid hyperplasia were evident in the spleen (74%) and Peyer's patches (64%). Lymphoid cell accumulations were prominent in the salivary glands (36%), periportal hepatic region (25%), lungs (32%), perivascular regions (36%), and kidney (21%). Cytomegalic inclusion body sialoadenitis was common (25%). Coccidiosis was evident in the intestinal tract (25%), kidney (25%), and muscle (21%). One neoplasm, a parahepatic haemangioma, was observed histologically.Mean body weights and lengths for virus-positive animals (33 g and 9.2 cm) and virus-negative animals (54 g and 12.2 cm) showed that virus-positive animals were smaller in weight and shorter in length. Since the age of the animals could not be determined, these differences remain unexplained.In comparison with virus-negative animals, virus-positive Mastomys had higher frequencies of splenic follicular hyperplasia (82% against 50%), myocarditis (79% against 29%), perivascular lymphoid cell accumulation (57% against 7%), myositis (50% against 14%), and cytomegalic inclusion body sialoadenitis (36% against 14%). The frequency of lymphoid hyperplasia of Peyer's patches was high in both groups of animals (71% and 57%).The presence of Lassa virus, small size, myocarditis, and lymphoid perivasculitis appeared to be interrelated, but larger and better controlled studies are required to elucidate the relationship.", "contents": "Lassa virus infection in Mastomys natalensis in Sierra Leone. Gross and microscopic findings in infected and uninfected animals. Pathological examinations of 28 wild-caught Mastomys natalensis from Sierra Leone, 14 of which were positive for Lassa virus by tissue culture, are reported. The high frequency of neoplastic and degenerative diseases observed among older animals in closed colonies of M. natalensis were not observed in the wild animals studied. This is probably a reflection of the age distribution of the study population, since the life expectancy of wild Mastomys is less than a year. Inflammatory lesions were nonetheless identified, some of which were similar to those described in laboratory colonies. Frequent lesions were myocarditis (54%), myositis (32%), interstitial pneumonitis (50%), intercapillary glomerulosclerosis (36%), and acute nephrosis (14%). Follicular and nodular lymphoid hyperplasia were evident in the spleen (74%) and Peyer's patches (64%). Lymphoid cell accumulations were prominent in the salivary glands (36%), periportal hepatic region (25%), lungs (32%), perivascular regions (36%), and kidney (21%). Cytomegalic inclusion body sialoadenitis was common (25%). Coccidiosis was evident in the intestinal tract (25%), kidney (25%), and muscle (21%). One neoplasm, a parahepatic haemangioma, was observed histologically.Mean body weights and lengths for virus-positive animals (33 g and 9.2 cm) and virus-negative animals (54 g and 12.2 cm) showed that virus-positive animals were smaller in weight and shorter in length. Since the age of the animals could not be determined, these differences remain unexplained.In comparison with virus-negative animals, virus-positive Mastomys had higher frequencies of splenic follicular hyperplasia (82% against 50%), myocarditis (79% against 29%), perivascular lymphoid cell accumulation (57% against 7%), myositis (50% against 14%), and cytomegalic inclusion body sialoadenitis (36% against 14%). The frequency of lymphoid hyperplasia of Peyer's patches was high in both groups of animals (71% and 57%).The presence of Lassa virus, small size, myocarditis, and lymphoid perivasculitis appeared to be interrelated, but larger and better controlled studies are required to elucidate the relationship."}
{"id": "PMID:1085222", "title": "Problems of rodent control in rural tropical areas.", "content": "Rodent control strategies, techniques, and research needs in rural tropical environments are reviewed and discussed with special reference to Mastomys natalensis, the possible reservoir of Lassa fever in West Africa.Public health rodent problems are far more serious and widespread in rural tropical areas than in developed countries. In the latter, only the commensal rodents constitute a major problem, whereas in rural tropical areas, native semidomestic species also serve as disease reservoirs and sources of infection to man. The success of rodent control programmes in developed countries depends in large part on the willingness and ability of people and governments to spend relatively large sums on research and control, on an acquired intolerance of people to rats and disease, and on a substantial economic base. These prerequisites are not usually to be found in rural tropical areas. Consequently, the rodent control techniques and programme organizations of developed countries are not directly applicable to such areas, even though the principles are the same. For this reason, it is suggested that a well-funded, integrated research and control programme should be undertaken in a known Lassa fever area, stressing public education, personnel training, and environmental management as well as rodenticidal approaches.", "contents": "Problems of rodent control in rural tropical areas. Rodent control strategies, techniques, and research needs in rural tropical environments are reviewed and discussed with special reference to Mastomys natalensis, the possible reservoir of Lassa fever in West Africa.Public health rodent problems are far more serious and widespread in rural tropical areas than in developed countries. In the latter, only the commensal rodents constitute a major problem, whereas in rural tropical areas, native semidomestic species also serve as disease reservoirs and sources of infection to man. The success of rodent control programmes in developed countries depends in large part on the willingness and ability of people and governments to spend relatively large sums on research and control, on an acquired intolerance of people to rats and disease, and on a substantial economic base. These prerequisites are not usually to be found in rural tropical areas. Consequently, the rodent control techniques and programme organizations of developed countries are not directly applicable to such areas, even though the principles are the same. For this reason, it is suggested that a well-funded, integrated research and control programme should be undertaken in a known Lassa fever area, stressing public education, personnel training, and environmental management as well as rodenticidal approaches."}
{"id": "PMID:1085223", "title": "Evaluation of demographic parameters of native rodent populations and implications for control.", "content": "The ecology of the multimammate mouse ,Mastomys natalensis, is reviewed and approximations are derived for the parameters governing population growth. By means of computer simulation, the relative importance of the timing of reproduction, the age class distribution or age structure of the population, the competition between Mastomys and Rattus, and the interaction with a predator are evaluated. Although each of these demographic or ecological factors modifies the fate of the Mastomys population, the greatest single impact results from a reproductive season that is divided into two parts rather than a single continuous reproductive season. Division into two parts, correlated with a similar distribution of rainfall, allows time for maturing of the young born early in the season and for production of young by them, thus adding to the momentum of population increase. The interaction of density-dependent factors controlling population growth, competition with another rodent, or predation by a Viverrid predator, may increase the growth rate of the Mastomys population or may depress population growth rate, even to the point of extinction. These simulation studies demonstrate the necessity for critical evaluation of the demographic parameters and ecological characteristics of a particular Mastomys population before an effective control programme can be designed. They also demonstrate, however, that if the programme is based upon sound ecological theory control can be effected.", "contents": "Evaluation of demographic parameters of native rodent populations and implications for control. The ecology of the multimammate mouse ,Mastomys natalensis, is reviewed and approximations are derived for the parameters governing population growth. By means of computer simulation, the relative importance of the timing of reproduction, the age class distribution or age structure of the population, the competition between Mastomys and Rattus, and the interaction with a predator are evaluated. Although each of these demographic or ecological factors modifies the fate of the Mastomys population, the greatest single impact results from a reproductive season that is divided into two parts rather than a single continuous reproductive season. Division into two parts, correlated with a similar distribution of rainfall, allows time for maturing of the young born early in the season and for production of young by them, thus adding to the momentum of population increase. The interaction of density-dependent factors controlling population growth, competition with another rodent, or predation by a Viverrid predator, may increase the growth rate of the Mastomys population or may depress population growth rate, even to the point of extinction. These simulation studies demonstrate the necessity for critical evaluation of the demographic parameters and ecological characteristics of a particular Mastomys population before an effective control programme can be designed. They also demonstrate, however, that if the programme is based upon sound ecological theory control can be effected."}
{"id": "PMID:1085224", "title": "Problems associated with the control of rodents in tropical Africa.", "content": "As elsewhere in the world, rodents are responsible for very considerable economic losses in tropical Africa because of their depredations on both growing crops and stored food products. Unfortunately, few accurate data are available on the extent of these losses but there is evidence that they are considerable. The public health importance of rodents, both as reservoirs and vectors of disease in tropical Africa, is also great; plague, leptospirosis, murine typhus, and Lassa fever are among the diseases associated with rodent hosts. Scientifically based rodent control programmes have been carried out in very few areas of Africa and there is urgent need for studies and demonstrations on rodent control in both urban and rural areas. The problems likely to be encountered are reviewed and methods of control proposed.", "contents": "Problems associated with the control of rodents in tropical Africa. As elsewhere in the world, rodents are responsible for very considerable economic losses in tropical Africa because of their depredations on both growing crops and stored food products. Unfortunately, few accurate data are available on the extent of these losses but there is evidence that they are considerable. The public health importance of rodents, both as reservoirs and vectors of disease in tropical Africa, is also great; plague, leptospirosis, murine typhus, and Lassa fever are among the diseases associated with rodent hosts. Scientifically based rodent control programmes have been carried out in very few areas of Africa and there is urgent need for studies and demonstrations on rodent control in both urban and rural areas. The problems likely to be encountered are reviewed and methods of control proposed."}
{"id": "PMID:1085225", "title": "Diagnosis of Lassa fever and the isolation and management of patients.", "content": "The clinical spectrum of Lassa fever is described and discussed in terms of the possible pathophysiological events involved. Early diagnosis is essential to permit prompt isolation of the potentially infectious patient. Lassa fever may be suspected on clinical grounds, but specific early diagnosis depends upon isolation of the virus. Virus isolation is best accomplished from serum obtained during the first 2 weeks of illness. The patterns of viraemia and virus excretion described in this paper are useful guidelines for determining the duration of patient isolation. Problems encountered in the isolation, management, and transport of the patient with Lassa fever are discussed.", "contents": "Diagnosis of Lassa fever and the isolation and management of patients. The clinical spectrum of Lassa fever is described and discussed in terms of the possible pathophysiological events involved. Early diagnosis is essential to permit prompt isolation of the potentially infectious patient. Lassa fever may be suspected on clinical grounds, but specific early diagnosis depends upon isolation of the virus. Virus isolation is best accomplished from serum obtained during the first 2 weeks of illness. The patterns of viraemia and virus excretion described in this paper are useful guidelines for determining the duration of patient isolation. Problems encountered in the isolation, management, and transport of the patient with Lassa fever are discussed."}
{"id": "PMID:1085226", "title": "Handling patients with suspected Lassa fever entering Great Britain.", "content": "A special isolation unit has been created at the Hospital for Tropical Diseases, London, for the reception of patients arriving in Great Britain from West Africa with suspected Lassa fever. The management of such patients and the operation of the unit are described. Experience during the first eighteen months of operation has been very satisfactory and valuable lessons have been learned concerning the diagnosis and treatment of Lassa fever and the nursing of patients.", "contents": "Handling patients with suspected Lassa fever entering Great Britain. A special isolation unit has been created at the Hospital for Tropical Diseases, London, for the reception of patients arriving in Great Britain from West Africa with suspected Lassa fever. The management of such patients and the operation of the unit are described. Experience during the first eighteen months of operation has been very satisfactory and valuable lessons have been learned concerning the diagnosis and treatment of Lassa fever and the nursing of patients."}
{"id": "PMID:1085227", "title": "Arenavirus chemotherapy--retrospect and prospect.", "content": "Two groups of compounds, identifiable by structural similarity, have been found to interfere with the in vitro replication of arenaviruses. All 4 members of the benzimidazole group contain dipolar fused benzene and 5-membered nitrogen-containing rings and share potential chelating ability through the different bidentate structures formed with their side-chains. The biological activity of one of these compounds, metisazone, has been shown to depend on the presence of divalent metals of the first transition series, Cu(++) being the most effective. Furthermore, whereas metisazone inactivates cell-free virus, two other members of the group, HBB and 1,2-bis(5-methoxy-1H-benzimidazol-2-yl)-1,2-ethanediol, act intracellularly. The site of action of the fourth member, SKF 30097, is not known. Using murine lymphocytic choriomeningitis infections as an in vivo model, the bisbenzimidazole derivative has been found to increase life-span without interfering with virus replication. Medication with SKF 30097 or metisazone and copper (2(+)) sulfate did not significantly or reproducibly change the expected day of death of the animals. The amantadine compounds of the second group have unusual symmetric structures with a 10-carbon cage. The parent compound acts intracellularly, while the site of action of an octachloro derivative is not known. Medication with the parent compound, but not the derivative, shortened the interval between LCM infection and death of the mouse. Tissue culture and animal screening of the many available derivatives in these two groups may uncover compounds more efficacious than those already examined.", "contents": "Arenavirus chemotherapy--retrospect and prospect. Two groups of compounds, identifiable by structural similarity, have been found to interfere with the in vitro replication of arenaviruses. All 4 members of the benzimidazole group contain dipolar fused benzene and 5-membered nitrogen-containing rings and share potential chelating ability through the different bidentate structures formed with their side-chains. The biological activity of one of these compounds, metisazone, has been shown to depend on the presence of divalent metals of the first transition series, Cu(++) being the most effective. Furthermore, whereas metisazone inactivates cell-free virus, two other members of the group, HBB and 1,2-bis(5-methoxy-1H-benzimidazol-2-yl)-1,2-ethanediol, act intracellularly. The site of action of the fourth member, SKF 30097, is not known. Using murine lymphocytic choriomeningitis infections as an in vivo model, the bisbenzimidazole derivative has been found to increase life-span without interfering with virus replication. Medication with SKF 30097 or metisazone and copper (2(+)) sulfate did not significantly or reproducibly change the expected day of death of the animals. The amantadine compounds of the second group have unusual symmetric structures with a 10-carbon cage. The parent compound acts intracellularly, while the site of action of an octachloro derivative is not known. Medication with the parent compound, but not the derivative, shortened the interval between LCM infection and death of the mouse. Tissue culture and animal screening of the many available derivatives in these two groups may uncover compounds more efficacious than those already examined."}
{"id": "PMID:1085228", "title": "The meaning of persistent infections in nature.", "content": "Viruses that cause persistent infections maintain themselves more effectively in nature than those causing acute and limited infections. There is a tendency for persistent viruses to evolve towards a state of minimal pathogenicity in the host. Vertical transmission, with integration of viral into host genome, represents the state of perfect parasitism.", "contents": "The meaning of persistent infections in nature. Viruses that cause persistent infections maintain themselves more effectively in nature than those causing acute and limited infections. There is a tendency for persistent viruses to evolve towards a state of minimal pathogenicity in the host. Vertical transmission, with integration of viral into host genome, represents the state of perfect parasitism."}
{"id": "PMID:1085229", "title": "Directions for future research on the pathogenesis of arenaviral infections.", "content": "The pathogenesis of arenavirus infection is considered separately for the haemorrhagic fever (HF) syndrome and for lymphocytic choriomeningitis (LCM) virus infection of rodents. Experimental models of HF have received only limited study, mainly because of the virulence of the causal agents. Two useful models (Junin virus in guinea-pigs and Machupo virus in rhesus monkeys) are now available and an attempt is made to delineate crucial questions for future studies, including the physiology of shock, disseminated intravascular coagulation, immune mediation of disease, efficacy of antibody in treatment, and relative utility of attenuated and inactivated vaccines. Immunobiologic problems currently under investigation in LCM virus infection include the mechanism of immune destruction of infected tissues, the H-2 restriction of in vitro T-cell-mediated lysis of infected target cells, the transient immunodepression that accompanies acute primary LCM virus infection, and the mechanism of T-cell-mediated clearance of virus from infected tissues following adoptive immunization of persistently infected carrier mice.", "contents": "Directions for future research on the pathogenesis of arenaviral infections. The pathogenesis of arenavirus infection is considered separately for the haemorrhagic fever (HF) syndrome and for lymphocytic choriomeningitis (LCM) virus infection of rodents. Experimental models of HF have received only limited study, mainly because of the virulence of the causal agents. Two useful models (Junin virus in guinea-pigs and Machupo virus in rhesus monkeys) are now available and an attempt is made to delineate crucial questions for future studies, including the physiology of shock, disseminated intravascular coagulation, immune mediation of disease, efficacy of antibody in treatment, and relative utility of attenuated and inactivated vaccines. Immunobiologic problems currently under investigation in LCM virus infection include the mechanism of immune destruction of infected tissues, the H-2 restriction of in vitro T-cell-mediated lysis of infected target cells, the transient immunodepression that accompanies acute primary LCM virus infection, and the mechanism of T-cell-mediated clearance of virus from infected tissues following adoptive immunization of persistently infected carrier mice."}
{"id": "PMID:1085230", "title": "The arenaviruses: some priorities for future research.", "content": "In this paper Lassa virus is used as a model in pinpointing priorities for future research on the arenaviruses. Suggestions for specific investigations and public health measures cover the detection of Lassa virus infection, the pathology and therapy of the disease, and its prevention and control.", "contents": "The arenaviruses: some priorities for future research. In this paper Lassa virus is used as a model in pinpointing priorities for future research on the arenaviruses. Suggestions for specific investigations and public health measures cover the detection of Lassa virus infection, the pathology and therapy of the disease, and its prevention and control."}
{"id": "PMID:1085232", "title": "Adipose tissue cellularity in human obesity.", "content": "All human obesity is characterised by adipocyte hypertrophy and when body weight exceeds 170 per cent of ideal, a maximum cell size of roughly twice normal is achieved. With greater severity, hyperplasia becomes increasingly manifest and when body weight exceeds 170 per cent of ideal, the degree of hyperplasia is well correlated with severity. Although severity and hypercellularity are often found in those with early onset of obesity, individuals can be found who are hypercellular but have had a later onset of obesity. Until the details of cellular development in man are more fully understood, the precise timing of 'critical' periods for cellular development must remain speculative.", "contents": "Adipose tissue cellularity in human obesity. All human obesity is characterised by adipocyte hypertrophy and when body weight exceeds 170 per cent of ideal, a maximum cell size of roughly twice normal is achieved. With greater severity, hyperplasia becomes increasingly manifest and when body weight exceeds 170 per cent of ideal, the degree of hyperplasia is well correlated with severity. Although severity and hypercellularity are often found in those with early onset of obesity, individuals can be found who are hypercellular but have had a later onset of obesity. Until the details of cellular development in man are more fully understood, the precise timing of 'critical' periods for cellular development must remain speculative."}
{"id": "PMID:1085235", "title": "Hidden' IgG antiglobulins in normal human serum.", "content": "Hidden antiglobulins reacting with whole rabbit immunoglobulin were found by radioassay or indirect haemagglutination after gel filtration or ultracentrifugation of normal serum. Suprisingly, both the IgM and IgG antiglobulins were present in the same macroglobulin fractions. The IgG antiglobulins could be dissociated into 7S components by separating the serum under acid conditions. Antiglobulin activity was a function of the Fab region.", "contents": "Hidden' IgG antiglobulins in normal human serum. Hidden antiglobulins reacting with whole rabbit immunoglobulin were found by radioassay or indirect haemagglutination after gel filtration or ultracentrifugation of normal serum. Suprisingly, both the IgM and IgG antiglobulins were present in the same macroglobulin fractions. The IgG antiglobulins could be dissociated into 7S components by separating the serum under acid conditions. Antiglobulin activity was a function of the Fab region."}
{"id": "PMID:1085236", "title": "A double antibody solid phase assay for DNA autoantibodies for clinical use.", "content": "DNA antoantibodies in serum will bind to antigen-coated polystyrene tubes and can be detected by radiolabelled anti-immunoglobulin. The method is quantitative, gives information on the antibody class and is not readily subject to interference by factors such as the size of the DNA, minor contamination of double-stranded with single-stranded DNA and the presence of materials which can combine \"non-specifically\" with the antigen. Double-stranded DNA gave good discrimination between SLE and rheumatoid arthritis but with single-stranded preparations approximately 40% of the RA patients showed elevated antibody values. Individual results obtained with the two antigens were compared and correlated with the Farr test and ANA titres. Surprisingly, a proportion of the SLE sera gave high background binding to  tubes coated with gelatine.", "contents": "A double antibody solid phase assay for DNA autoantibodies for clinical use. DNA antoantibodies in serum will bind to antigen-coated polystyrene tubes and can be detected by radiolabelled anti-immunoglobulin. The method is quantitative, gives information on the antibody class and is not readily subject to interference by factors such as the size of the DNA, minor contamination of double-stranded with single-stranded DNA and the presence of materials which can combine \"non-specifically\" with the antigen. Double-stranded DNA gave good discrimination between SLE and rheumatoid arthritis but with single-stranded preparations approximately 40% of the RA patients showed elevated antibody values. Individual results obtained with the two antigens were compared and correlated with the Farr test and ANA titres. Surprisingly, a proportion of the SLE sera gave high background binding to  tubes coated with gelatine."}
{"id": "PMID:1085237", "title": "Acute energy deprivation in man: effect on cell-mediated immunological reactions.", "content": "The effects of 10 days of total energy deprivation on lymphocyte functions and cell-mediated immunity was evaluated in fourteen healthy, normal-weighted males. Lymphocytes from seven of the subjects were tested in vitro. A significant depression was noted of the DNA synthesis after stimulation with pokeweed mitogen and PPD while there was no effect on the response to concanavalin A. No change was noted in the percentages and total numbers of circulating B and T lymphocytes and monocytes. The delayed skin reactivity following intracutaneous PPD and mumps antigen was not different from non-starving control subjects.", "contents": "Acute energy deprivation in man: effect on cell-mediated immunological reactions. The effects of 10 days of total energy deprivation on lymphocyte functions and cell-mediated immunity was evaluated in fourteen healthy, normal-weighted males. Lymphocytes from seven of the subjects were tested in vitro. A significant depression was noted of the DNA synthesis after stimulation with pokeweed mitogen and PPD while there was no effect on the response to concanavalin A. No change was noted in the percentages and total numbers of circulating B and T lymphocytes and monocytes. The delayed skin reactivity following intracutaneous PPD and mumps antigen was not different from non-starving control subjects."}
{"id": "PMID:1085238", "title": "Rosette-formation with mouse erythrocytes. II. A marker for human B and non-T lymphocytes.", "content": "Peripheral blood lymphocytes from healthy control subjects were studied for spontaneous rosette-formation with mouse erythrocytes. The mean percentage of mouse erythrocyte rosette-forming cells (MRFC) in sixty-three subjects was 7.4X3.5; after neuraminidase treatment olymphocytes the mean percentage of mouse erythrocyte rosette-forming cells(NMRFC) was 17.2X5.6. Thymus cell suspensions made only occasional rosettes with mouse erythrocytes. There was no effect of neuraminidase treatment of thymocytes on mouse erythrocyte rosette-formation. Double labelling experiments with other cell surface markers confirmed this binding of mouse erythrocytes to be a B-cell characteristic. Monocytes and granulocytes do not bind mouse erythrocytes. It appears that there is a distinct receptor for the binding of mouse erythrocytes on the surface of lymphocytes carrying surface immunoglobulins; neuraminidase treatment of lymphocytes permits this binding to occur also on virtually all the non-T cells B cells plus the third population cells).", "contents": "Rosette-formation with mouse erythrocytes. II. A marker for human B and non-T lymphocytes. Peripheral blood lymphocytes from healthy control subjects were studied for spontaneous rosette-formation with mouse erythrocytes. The mean percentage of mouse erythrocyte rosette-forming cells (MRFC) in sixty-three subjects was 7.4X3.5; after neuraminidase treatment olymphocytes the mean percentage of mouse erythrocyte rosette-forming cells(NMRFC) was 17.2X5.6. Thymus cell suspensions made only occasional rosettes with mouse erythrocytes. There was no effect of neuraminidase treatment of thymocytes on mouse erythrocyte rosette-formation. Double labelling experiments with other cell surface markers confirmed this binding of mouse erythrocytes to be a B-cell characteristic. Monocytes and granulocytes do not bind mouse erythrocytes. It appears that there is a distinct receptor for the binding of mouse erythrocytes on the surface of lymphocytes carrying surface immunoglobulins; neuraminidase treatment of lymphocytes permits this binding to occur also on virtually all the non-T cells B cells plus the third population cells)."}
{"id": "PMID:1085239", "title": "In vitro response of bovine thoracic duct lymphocyte to phytohaemagglutinin following adult thymectomy.", "content": "The effect of adult thymectomy on the thoracic duct lymphocyte population of yearling calves has been investigated. Four to 6 weeks after thymectomy animals showed significantly reduced thoracic duct lymphocyte concentrations when compared to non-thymectomized controls. In addition, phytohaemagglutinin responsiveness of thoracic duct lymphocytes, measured by (3H) thymidine uptake, was significantly decreased following adult thymectomy. However, this decreased response to PHA was not accompanied by a change in spontaneous isotope incorporation. It is concluded that adult thymectomy in the bovine probably leads to a reduction in the number of PHA responsive T cells in the thoracic duct lymph.", "contents": "In vitro response of bovine thoracic duct lymphocyte to phytohaemagglutinin following adult thymectomy. The effect of adult thymectomy on the thoracic duct lymphocyte population of yearling calves has been investigated. Four to 6 weeks after thymectomy animals showed significantly reduced thoracic duct lymphocyte concentrations when compared to non-thymectomized controls. In addition, phytohaemagglutinin responsiveness of thoracic duct lymphocytes, measured by (3H) thymidine uptake, was significantly decreased following adult thymectomy. However, this decreased response to PHA was not accompanied by a change in spontaneous isotope incorporation. It is concluded that adult thymectomy in the bovine probably leads to a reduction in the number of PHA responsive T cells in the thoracic duct lymph."}
{"id": "PMID:1085242", "title": "Stress ulcer: a continuing dilemma.", "content": "The disease is painless and presents as a massive upper GI hemorrhage. Early diagnosis and rapid treatment are mandatory for patient survival. A variety of nonoperative treatment regimens are available, none demonstrating a clearcut superiority. Because the incidence of rebleeding is high, operative intervention is frequently required. The appropriate surgical procedure is unclear, although most surgeons perfer a generous subtotal gastrectomy with truncal vagotomy. Unquestionably, the best treatment is prevention.", "contents": "Stress ulcer: a continuing dilemma. The disease is painless and presents as a massive upper GI hemorrhage. Early diagnosis and rapid treatment are mandatory for patient survival. A variety of nonoperative treatment regimens are available, none demonstrating a clearcut superiority. Because the incidence of rebleeding is high, operative intervention is frequently required. The appropriate surgical procedure is unclear, although most surgeons perfer a generous subtotal gastrectomy with truncal vagotomy. Unquestionably, the best treatment is prevention."}
{"id": "PMID:1085247", "title": "[Clinical results of laser-coagulation in gastro-intestinal bleeding (author's transl)].", "content": "Endoscopic laser-coagulation was used 94 times in 14 patients to stop gastro-intestinal bleeding or coagulate potential bleeding sources during non-bleeding intervals. Lasting haemostasis was achieved in three cases of incomplete gastric erosion, one of gastric ulcer, one of duodenal ulcer and one of bleeding after antral rugectomy. It failed to stop severe arterial bleeding in a case of gastric carcinoma. Lasting haemostasis was achieved in six haemangiomas of the colon, one case of angiomatosis of the antrum with 62 individual sites, two of angiodysplasia of the colon, 17 lesions in Osler's disease of the oesophagus (2 cases), stomach (10) and duodenum (5). The procedure was performed in the course of diagnostic endoscopy with an argonion laser developed by the authors, which has a density of 0.7-1.3 W/mm2. No complications have been observed so far.", "contents": "[Clinical results of laser-coagulation in gastro-intestinal bleeding (author's transl)]. Endoscopic laser-coagulation was used 94 times in 14 patients to stop gastro-intestinal bleeding or coagulate potential bleeding sources during non-bleeding intervals. Lasting haemostasis was achieved in three cases of incomplete gastric erosion, one of gastric ulcer, one of duodenal ulcer and one of bleeding after antral rugectomy. It failed to stop severe arterial bleeding in a case of gastric carcinoma. Lasting haemostasis was achieved in six haemangiomas of the colon, one case of angiomatosis of the antrum with 62 individual sites, two of angiodysplasia of the colon, 17 lesions in Osler's disease of the oesophagus (2 cases), stomach (10) and duodenum (5). The procedure was performed in the course of diagnostic endoscopy with an argonion laser developed by the authors, which has a density of 0.7-1.3 W/mm2. No complications have been observed so far."}
{"id": "PMID:1085249", "title": "Inhibitory effect of 5-hydroxytryptophane on the induction of persistent estrus by androgen in the rat.", "content": "Effects of monoamines or its precursors on the induction of persistent estrus by testosterone propionate (T.P.) were studied in Wistar strain female rats. Daily treatment with 5-hydroxytryptophane (5-HTP) for the first 10 days of life delayed the occurrence of persistent estrus in rats given T.P. at 4 days of age. The onset of persistent estrus occurred in this group of animals at 67.1+/-2.5 days of age compared with 45.1+/-1.4 days of age for the saline treated control group. The role of hypothalamic monoamines in contributing to the induction of persistent estrus by T.P. treatment of neonatal rats is discussed.", "contents": "Inhibitory effect of 5-hydroxytryptophane on the induction of persistent estrus by androgen in the rat. Effects of monoamines or its precursors on the induction of persistent estrus by testosterone propionate (T.P.) were studied in Wistar strain female rats. Daily treatment with 5-hydroxytryptophane (5-HTP) for the first 10 days of life delayed the occurrence of persistent estrus in rats given T.P. at 4 days of age. The onset of persistent estrus occurred in this group of animals at 67.1+/-2.5 days of age compared with 45.1+/-1.4 days of age for the saline treated control group. The role of hypothalamic monoamines in contributing to the induction of persistent estrus by T.P. treatment of neonatal rats is discussed."}
{"id": "PMID:1085250", "title": "Effect of cortisol on the release and retention of labelled DNA in the thymus and spleen of mice.", "content": "The lymphocytolytic effect of different doses of cortisol was studied in the thymus and spleen of mice previously injected with 3H-thymidine. The results indicate that in thymus the fraction of labelled cells was more resistant to cortisol than the unlabelled cell population. The release of DNA into the fraction of DNA soluble in 0.14 M NaC1 was delayed suggesting that cortisol controls indirectly the lymphocytolytic process. Up to 24 hours after administration of cortisol the loss of labelled spleen cells significantly exceeded the loss of unlabelled cells. The time course of the release of labelled DNA into the fraction of DNA soluble in 0.14 M NaC1 indicates that a fraction of labelled DNA was rapidly removed from the spleen after injection of cortisol.", "contents": "Effect of cortisol on the release and retention of labelled DNA in the thymus and spleen of mice. The lymphocytolytic effect of different doses of cortisol was studied in the thymus and spleen of mice previously injected with 3H-thymidine. The results indicate that in thymus the fraction of labelled cells was more resistant to cortisol than the unlabelled cell population. The release of DNA into the fraction of DNA soluble in 0.14 M NaC1 was delayed suggesting that cortisol controls indirectly the lymphocytolytic process. Up to 24 hours after administration of cortisol the loss of labelled spleen cells significantly exceeded the loss of unlabelled cells. The time course of the release of labelled DNA into the fraction of DNA soluble in 0.14 M NaC1 indicates that a fraction of labelled DNA was rapidly removed from the spleen after injection of cortisol."}
{"id": "PMID:1085251", "title": "Experimental coronary artery bypass operation. Myocardial blood flow, ventricular performance and regional myocardial function.", "content": "Myocardial blood flow and ventricular function was studied in seven dogs with chronic myocardial ischemia before and after coronary bypass grafting. Restoring blood flow in an area of 25% of the anterior wall of the left ventricle did not significantly improve overall ventricular function. Assessment of intramyocardial pressure as an index of regional myocardial function revealed a consistent enhancement of myocardial contraction at rest, and under pharmacological stress of the heart.", "contents": "Experimental coronary artery bypass operation. Myocardial blood flow, ventricular performance and regional myocardial function. Myocardial blood flow and ventricular function was studied in seven dogs with chronic myocardial ischemia before and after coronary bypass grafting. Restoring blood flow in an area of 25% of the anterior wall of the left ventricle did not significantly improve overall ventricular function. Assessment of intramyocardial pressure as an index of regional myocardial function revealed a consistent enhancement of myocardial contraction at rest, and under pharmacological stress of the heart."}
{"id": "PMID:1085253", "title": "Tryptophan hydroxylase in discrete brain nuclei: comparison of activity in vitro and in vivo.", "content": "The increase of 5-hydroxytryptophan after treatment with the decarboxylase inhibitor Ro 4/4602 was 30 times greater in the raphe nuclei (which contain serotonergic cell bodies) than the hippocampus or caudate (which contain serotonergic terminals). Pretreatment with chlorimipramine reduced the 5-hydroxytryptophan accumulation in the median but not dorsal raphe. The large amount of tryptophan hydroxylase in cell bodies appears to be active in vivo. The serotonin rapidly turning over there may have a functional role.", "contents": "Tryptophan hydroxylase in discrete brain nuclei: comparison of activity in vitro and in vivo. The increase of 5-hydroxytryptophan after treatment with the decarboxylase inhibitor Ro 4/4602 was 30 times greater in the raphe nuclei (which contain serotonergic cell bodies) than the hippocampus or caudate (which contain serotonergic terminals). Pretreatment with chlorimipramine reduced the 5-hydroxytryptophan accumulation in the median but not dorsal raphe. The large amount of tryptophan hydroxylase in cell bodies appears to be active in vivo. The serotonin rapidly turning over there may have a functional role."}
{"id": "PMID:1085254", "title": "Effect of aprotinin, EACA and heparin on growth and vasopeptide system of Murphy-Sturm lymphosarcoma.", "content": "The effect of aprotinin, EACA and heparin on the growth of the transplanted rodent Murphy-Sturm lymphosarcoma and on several components of the tumor kinin-forming enzyme system was studied. Therapy was administered for 15 days, 3 times daily, one day after tumor transplant. Tumor weights and biochemical parameters (prekallikrein, kininogen and kininase) were measured on the 8th, 10th, 12th and 15th day of therapy. Both aprotinin and heparin significantly inhibited (p less than 0.01) tumor growth by the 15th day, compared to control, saline-administered animals. EACA did not affect the tumor growth rate at any time period. Tumor prekallikrein and kininogen levels were significantly higher (p less than 0.01) in the aprotinin-treated animals. Kininogen levels were higher in the EACA-treated tumors during the latter phase of growth. None of the agents affected the increase in tumor kininase activity that occured during the growth of the tumors. The tumor inhibition and biochemical data suggest the involvement of proteases in the neoplastic process.", "contents": "Effect of aprotinin, EACA and heparin on growth and vasopeptide system of Murphy-Sturm lymphosarcoma. The effect of aprotinin, EACA and heparin on the growth of the transplanted rodent Murphy-Sturm lymphosarcoma and on several components of the tumor kinin-forming enzyme system was studied. Therapy was administered for 15 days, 3 times daily, one day after tumor transplant. Tumor weights and biochemical parameters (prekallikrein, kininogen and kininase) were measured on the 8th, 10th, 12th and 15th day of therapy. Both aprotinin and heparin significantly inhibited (p less than 0.01) tumor growth by the 15th day, compared to control, saline-administered animals. EACA did not affect the tumor growth rate at any time period. Tumor prekallikrein and kininogen levels were significantly higher (p less than 0.01) in the aprotinin-treated animals. Kininogen levels were higher in the EACA-treated tumors during the latter phase of growth. None of the agents affected the increase in tumor kininase activity that occured during the growth of the tumors. The tumor inhibition and biochemical data suggest the involvement of proteases in the neoplastic process."}
{"id": "PMID:1085258", "title": "Characteristics of B-lymphocyte colony growth in vitro.", "content": "A cloning system in semisolid agar has been developed which supports colony formation by mouse B-lymphocytes. Colonies appear to be clones and colony cells synthesize immunoglobulin. Approximately one cell in 50 in the spleen or lymph nodes is capable of forming a colony and colony-forming cells appear to have the properties of B-lymphocytes. Colony formation is dependent on mercaptoethanol but the possible additional involvement of a specific B-lymphocyte regulatory factor has not been excluded.", "contents": "Characteristics of B-lymphocyte colony growth in vitro. A cloning system in semisolid agar has been developed which supports colony formation by mouse B-lymphocytes. Colonies appear to be clones and colony cells synthesize immunoglobulin. Approximately one cell in 50 in the spleen or lymph nodes is capable of forming a colony and colony-forming cells appear to have the properties of B-lymphocytes. Colony formation is dependent on mercaptoethanol but the possible additional involvement of a specific B-lymphocyte regulatory factor has not been excluded."}
{"id": "PMID:1085260", "title": "Reactivation by glycerol and ethylene glycol of inactivated delta-aminolevulinic acid synthetase of Rhodopseudomonas spheroides.", "content": "Reactivation effects by glycerol and ethylene glycol of inactivated ALA synthetase of R. spheroids were observed. Accompanying the reactivation of the inactivated enzyme, Km value for PLP decreased to levels similar to those of the freshly prepared enzyme.", "contents": "Reactivation by glycerol and ethylene glycol of inactivated delta-aminolevulinic acid synthetase of Rhodopseudomonas spheroides. Reactivation effects by glycerol and ethylene glycol of inactivated ALA synthetase of R. spheroids were observed. Accompanying the reactivation of the inactivated enzyme, Km value for PLP decreased to levels similar to those of the freshly prepared enzyme."}
{"id": "PMID:1085261", "title": "Latency of taste nerve signals in frog (Rana catesbeiana).", "content": "The latency of frog gustatory neural impulses to 1.0 M NaCl was a mean of 86 msec. Electrical stimulation of taste cell membranes produced gustatory neural impulses with the mean 5 msec latency. It is concluded that most of the 86 msec latency of taste nerve responses to 1.0 N NaCl is due to the latency of taste receptor potential following the onset of gustatory stimulation.", "contents": "Latency of taste nerve signals in frog (Rana catesbeiana). The latency of frog gustatory neural impulses to 1.0 M NaCl was a mean of 86 msec. Electrical stimulation of taste cell membranes produced gustatory neural impulses with the mean 5 msec latency. It is concluded that most of the 86 msec latency of taste nerve responses to 1.0 N NaCl is due to the latency of taste receptor potential following the onset of gustatory stimulation."}
{"id": "PMID:1085262", "title": "Elemental distribution of Na, P, Cl and K in different structures of myelinated nerve of Rana esculenta.", "content": "Measurements of the distribution of Na, P, Cl and K were performed in different structures of the myelinated nerve. Whereas the axon shows a typical intracellular distribution pattern for Na, Cl and K, the interstitial space and the myelin sheath show a typical extracellular pattern. These measurements have demonstrated that Na is present in the myelin sheath close to the node of Ranvier.", "contents": "Elemental distribution of Na, P, Cl and K in different structures of myelinated nerve of Rana esculenta. Measurements of the distribution of Na, P, Cl and K were performed in different structures of the myelinated nerve. Whereas the axon shows a typical intracellular distribution pattern for Na, Cl and K, the interstitial space and the myelin sheath show a typical extracellular pattern. These measurements have demonstrated that Na is present in the myelin sheath close to the node of Ranvier."}
{"id": "PMID:1085266", "title": "The effects of neonatal vestibular stimulation on adult growth and emotional reactivity in BALB/c mice.", "content": "Two experiments were conducted to determine the effects of neonatal vestibular stimulation on the subsequent development and behavior in BALB/c mice. No evidence appeared to suggest that neonatal vestibular stimulation alters either the growth pattern or the ontogenesis of emotional reactivity.", "contents": "The effects of neonatal vestibular stimulation on adult growth and emotional reactivity in BALB/c mice. Two experiments were conducted to determine the effects of neonatal vestibular stimulation on the subsequent development and behavior in BALB/c mice. No evidence appeared to suggest that neonatal vestibular stimulation alters either the growth pattern or the ontogenesis of emotional reactivity."}
{"id": "PMID:1085267", "title": "Portal hypertension and primary biliary cirrhosis.", "content": "Portal hypertension has been regarded as an uncommon and late complication of primary biliary cirrhosis (PBC). 24 patients with PBC were investigated for portal hypertension. Esophageal varices were present in 20, 50, and 90% of the patients 1, 3, and 9 years, respectively, after the onset of pruritus and/or jaundice. Portal hypertension was responsible for gastrointestinal bleedings in 11 patients; bleeding was the first clinical manifestation of PBC in two of them. Wedged hepatic venous pressure was increased in all the patients with portal hypertension whether regenerative nodules were present or absent. Portacaval shunt was performed in five patients and was well tolerated in three of them. It is concluded that (a) portal hypertension is common in PBC; (b) the intrahepatic block is of the so-called postsinusoidal type, even in patients without regenerative nodules; (c) gastro-intestinal bleeding due to portal hypertension occurs in about half of the patients and may be the first manifestation of PBC; (d) portacaval shunt seems to be indicated when gastro-intestinal bleeding occurs in earlier stage of the disease.", "contents": "Portal hypertension and primary biliary cirrhosis. Portal hypertension has been regarded as an uncommon and late complication of primary biliary cirrhosis (PBC). 24 patients with PBC were investigated for portal hypertension. Esophageal varices were present in 20, 50, and 90% of the patients 1, 3, and 9 years, respectively, after the onset of pruritus and/or jaundice. Portal hypertension was responsible for gastrointestinal bleedings in 11 patients; bleeding was the first clinical manifestation of PBC in two of them. Wedged hepatic venous pressure was increased in all the patients with portal hypertension whether regenerative nodules were present or absent. Portacaval shunt was performed in five patients and was well tolerated in three of them. It is concluded that (a) portal hypertension is common in PBC; (b) the intrahepatic block is of the so-called postsinusoidal type, even in patients without regenerative nodules; (c) gastro-intestinal bleeding due to portal hypertension occurs in about half of the patients and may be the first manifestation of PBC; (d) portacaval shunt seems to be indicated when gastro-intestinal bleeding occurs in earlier stage of the disease."}
{"id": "PMID:1085268", "title": "High power argon laser treatment via standard endoscopes. I. A preliminary study of efficacy in control of experimental erosive bleeding.", "content": "With minimal transmission loss a high power argon laser (10 w) was coupled to a waveguide consisting of a flexible, single, coated, quartz fiber encased in a protective polyethylene tube. This waveguide can be passed down the biopsy channel of any standard fiberoptic endoscope. An aiming light improves accuracy. Safety devices were developed to protect the subject and the operator. Each of 8 heparinized mongrel dogs had three superficial erosions created endoscopically in the fundal gland mucosa by a jet of warm 0.1 N HC1. In each animal two lesions were photocoagulated with the laser and the third was left as a control. The animals were killed at 0, 4, 7, 10, and 14 days, and the erosions were examined histologically. By 14 days all lesions were covered by normal surface epithelium. Parietal and chief cells had returned to untreated erosions at 14 days but not to all lasered lesions. Only rarely did the lasered lesions penetrate through the muscularis mucosae into the submucosa. High power argon laser photocoagulation is now feasible through standard endoscopes. These data are sufficiently promising to encourage further evaluation of laser photocoagulation in a variety of animal models.", "contents": "High power argon laser treatment via standard endoscopes. I. A preliminary study of efficacy in control of experimental erosive bleeding. With minimal transmission loss a high power argon laser (10 w) was coupled to a waveguide consisting of a flexible, single, coated, quartz fiber encased in a protective polyethylene tube. This waveguide can be passed down the biopsy channel of any standard fiberoptic endoscope. An aiming light improves accuracy. Safety devices were developed to protect the subject and the operator. Each of 8 heparinized mongrel dogs had three superficial erosions created endoscopically in the fundal gland mucosa by a jet of warm 0.1 N HC1. In each animal two lesions were photocoagulated with the laser and the third was left as a control. The animals were killed at 0, 4, 7, 10, and 14 days, and the erosions were examined histologically. By 14 days all lesions were covered by normal surface epithelium. Parietal and chief cells had returned to untreated erosions at 14 days but not to all lasered lesions. Only rarely did the lasered lesions penetrate through the muscularis mucosae into the submucosa. High power argon laser photocoagulation is now feasible through standard endoscopes. These data are sufficiently promising to encourage further evaluation of laser photocoagulation in a variety of animal models."}
{"id": "PMID:1085269", "title": "Pathogenesis of bleeding colonic diverticulosis.", "content": "The cause of bleeding was found in 8 of 10 cases of colonic diverticulosis associated with severe rectal hemorrhage by using arteriographic and microangiographic techniques to localize the site and serial histological sections to study the lesion. Strikingly consistent changes were identified. These are related to the characteristic angioarchitecture of colonic diverticula. These changes include asymmetric rupture of the vas rectum toward the lumen of the diverticulum precisely at its dome or antimesenteric margin; conspicuous eccentric intimal thickening of the vas rectum, often with medial thinning of duplication of the internal elastic lamina at and near the bleeding point; and general absence of diverticulitis. Control colonic diverticula demonstrated normal structures or, only occasionally, minimal eccentric intimal thickening in their associated vasa recta. This suggests that traumatic factors arising within the diverticular or colonic lumen induce asymmetric intimal proliferation and scarring of the associated vasa recta, predisposing to rupture and massive bleeding.", "contents": "Pathogenesis of bleeding colonic diverticulosis. The cause of bleeding was found in 8 of 10 cases of colonic diverticulosis associated with severe rectal hemorrhage by using arteriographic and microangiographic techniques to localize the site and serial histological sections to study the lesion. Strikingly consistent changes were identified. These are related to the characteristic angioarchitecture of colonic diverticula. These changes include asymmetric rupture of the vas rectum toward the lumen of the diverticulum precisely at its dome or antimesenteric margin; conspicuous eccentric intimal thickening of the vas rectum, often with medial thinning of duplication of the internal elastic lamina at and near the bleeding point; and general absence of diverticulitis. Control colonic diverticula demonstrated normal structures or, only occasionally, minimal eccentric intimal thickening in their associated vasa recta. This suggests that traumatic factors arising within the diverticular or colonic lumen induce asymmetric intimal proliferation and scarring of the associated vasa recta, predisposing to rupture and massive bleeding."}
{"id": "PMID:1085270", "title": "alpha-1-antitrypsin deficiency in liver disease: the extent of the problem.", "content": "A prospective screening program was undertaken at the Royal Free Hospital, London, to ascertain the incidence of alpha-1-antitrypsin (AAT) deficiency in patients with liver disease. Quantitative determinations of serum alpha-1-antitrypsin were performed on 469 patients with hepatobiliary disease and 98 subjects with no known liver disease. Sera with low values of AAT were phenotyped. The homozygous state was rare and comprised only 1% of the patients with liver disease. All of the 5 homozygous deficient (ZZ phenotype) patients had a history of neonatal liver disease. Other phenotypes (partial deficiency) were found in 4.7% of patients with liver disease and 6.1% of subjects with normal liver function. Types of liver disease in the patients with other phenotypes were widely varied. Routine determination of serum AAT level and phenotype and special staining for AAT in liver biopsies in all adults with liver disease appears unnecessary. Investigation of possible AAT deficiency should be carried out, however, in children and young adults, in those with a history of neonatal liver disease, and possibly in all patients with liver disease of unknown aetiology.", "contents": "alpha-1-antitrypsin deficiency in liver disease: the extent of the problem. A prospective screening program was undertaken at the Royal Free Hospital, London, to ascertain the incidence of alpha-1-antitrypsin (AAT) deficiency in patients with liver disease. Quantitative determinations of serum alpha-1-antitrypsin were performed on 469 patients with hepatobiliary disease and 98 subjects with no known liver disease. Sera with low values of AAT were phenotyped. The homozygous state was rare and comprised only 1% of the patients with liver disease. All of the 5 homozygous deficient (ZZ phenotype) patients had a history of neonatal liver disease. Other phenotypes (partial deficiency) were found in 4.7% of patients with liver disease and 6.1% of subjects with normal liver function. Types of liver disease in the patients with other phenotypes were widely varied. Routine determination of serum AAT level and phenotype and special staining for AAT in liver biopsies in all adults with liver disease appears unnecessary. Investigation of possible AAT deficiency should be carried out, however, in children and young adults, in those with a history of neonatal liver disease, and possibly in all patients with liver disease of unknown aetiology."}
{"id": "PMID:1085280", "title": "Immunocompetent cells in man. IV. The inhibition of pokeweed mitogen-induced blastogenesis of normal human peripheral leukocytes by trypsin: a B-cell mitogen of mouse, but not of human, lymphocytes.", "content": "Normal human peripheral blood lymphocytes were tested for their blastogenic response in vitro to trypsin. Although it has been shown by other investigators that mouse B lymphocytes are stimulated trypsin to undergo blastogenesis, we have shown that human lymphocytes are not stimulated by trypsin. Paradoxically, trypsin inhibits the response of human lymphocytes to pokeweed mitogen, a B-cell stimulat, without significantly affecting the response of these cells to phytohemagglutinin, a T-cell stimulant. These results serve to underline the functional dissimilarity of mouse spleen cells and human circulating lymphocytes, at least with respect to their blastogenic responses to mitogenic agents.", "contents": "Immunocompetent cells in man. IV. The inhibition of pokeweed mitogen-induced blastogenesis of normal human peripheral leukocytes by trypsin: a B-cell mitogen of mouse, but not of human, lymphocytes. Normal human peripheral blood lymphocytes were tested for their blastogenic response in vitro to trypsin. Although it has been shown by other investigators that mouse B lymphocytes are stimulated trypsin to undergo blastogenesis, we have shown that human lymphocytes are not stimulated by trypsin. Paradoxically, trypsin inhibits the response of human lymphocytes to pokeweed mitogen, a B-cell stimulat, without significantly affecting the response of these cells to phytohemagglutinin, a T-cell stimulant. These results serve to underline the functional dissimilarity of mouse spleen cells and human circulating lymphocytes, at least with respect to their blastogenic responses to mitogenic agents."}
{"id": "PMID:1085281", "title": "Heterogeneity of the protein moiety of carcinoembryonic antigens.", "content": "N-terminal amino acid sequences were determined for the protein moiety of carcinoembryonic antigen (CEA) isolated from three colon cancers that had metastasized to the liver. The results showed that the polypeptide portion of CEA preparations isolated from different tumors are not identical. Furthermore, each CEA preparation appeared to be heterogeneous within itself, since multiple amino acid residues were recovered at most of the positions following stepwise cleavage of the protein moiety of CEA molecules by automatic Edman degradation.", "contents": "Heterogeneity of the protein moiety of carcinoembryonic antigens. N-terminal amino acid sequences were determined for the protein moiety of carcinoembryonic antigen (CEA) isolated from three colon cancers that had metastasized to the liver. The results showed that the polypeptide portion of CEA preparations isolated from different tumors are not identical. Furthermore, each CEA preparation appeared to be heterogeneous within itself, since multiple amino acid residues were recovered at most of the positions following stepwise cleavage of the protein moiety of CEA molecules by automatic Edman degradation."}
{"id": "PMID:1085282", "title": "Secondary cytotoxic cell response to lymphocytic choriomeningitis virus II. Nature and specificity of effector cells.", "content": "The method described in the previous paper was used to induce secondary responses in spleen cells from CBA/H mice, pre-primed with lymphocytic choriomeningitis (LCM) virus by culturing them with LCM-infected peritoneal cells. The cytolytic effector cells thus generated have been characterized. Effector cells were sensitive to treatment with anti-theta ascitic fluid and complement. Separation procedures based on rosetting of certain categories of lymphocytes with sheep red cells through an Isopaque-Ficoll gradient indicated that effector cells lacked surface immunoglobulin and generally did not bear Fc receptors. Cytolytic activity was restricted by the H-2 gene complex. Killing had single-hit characteristics. All these results suggested that the cells from memory cultures mediating cytolysis were T cells. There was evidence for two T cell subsets, a major subpopulation directed against antigens on infected targets and a minor one directed against antigens on uninfected, H-2-compatible targets. Specificity was present at the infected cell:memory responder and killer:target levels between LCM virus (an arenavirus) and ectromelia virus (a poxvirus).", "contents": "Secondary cytotoxic cell response to lymphocytic choriomeningitis virus II. Nature and specificity of effector cells. The method described in the previous paper was used to induce secondary responses in spleen cells from CBA/H mice, pre-primed with lymphocytic choriomeningitis (LCM) virus by culturing them with LCM-infected peritoneal cells. The cytolytic effector cells thus generated have been characterized. Effector cells were sensitive to treatment with anti-theta ascitic fluid and complement. Separation procedures based on rosetting of certain categories of lymphocytes with sheep red cells through an Isopaque-Ficoll gradient indicated that effector cells lacked surface immunoglobulin and generally did not bear Fc receptors. Cytolytic activity was restricted by the H-2 gene complex. Killing had single-hit characteristics. All these results suggested that the cells from memory cultures mediating cytolysis were T cells. There was evidence for two T cell subsets, a major subpopulation directed against antigens on infected targets and a minor one directed against antigens on uninfected, H-2-compatible targets. Specificity was present at the infected cell:memory responder and killer:target levels between LCM virus (an arenavirus) and ectromelia virus (a poxvirus)."}
{"id": "PMID:1085283", "title": "Preferential generation of killer or helper T-lymphocyte activity directed to the tumour-associated transplantation antigens.", "content": "Induction of killer and helper T-cell activities towards transplantation antigens of two tumour cell lines was analysed in the allogeneic and syngeneic host combinations. The lymphoid cells from C57BL/6 mice immunized with allogeneic viable or mitomycin C-treated X-5563 plasmacytoma cells derived from C3H/He mice revealed both killer and helper T-cell activities against alloantigens, whereas cells from mice immunized with tumour cells killed by a freezing and thawing procedure revealed predominantly helper T-cell activity. On the other hand, when C3H/He mice were immunized with viable syngeneic X-5563 plasmacytoma or MM102 mammary tumour cells, the former generated preferentially killer T-cell activity, whereas the latter induced predominantly helper T-cell activity against tumour-associated transplantation antigens. Thus, immunization with transplantation antigen(s) does not always induce both helper and killer T-cell activities in parallel, but a certain antigenic system induces predominantly one type of T-cell response, thus indicating that two distinct subsets of helper and killer T cells against the transplantation antigen(s) can be raised independently without an absolute requirement of collaboration between these different T-cells subsets.", "contents": "Preferential generation of killer or helper T-lymphocyte activity directed to the tumour-associated transplantation antigens. Induction of killer and helper T-cell activities towards transplantation antigens of two tumour cell lines was analysed in the allogeneic and syngeneic host combinations. The lymphoid cells from C57BL/6 mice immunized with allogeneic viable or mitomycin C-treated X-5563 plasmacytoma cells derived from C3H/He mice revealed both killer and helper T-cell activities against alloantigens, whereas cells from mice immunized with tumour cells killed by a freezing and thawing procedure revealed predominantly helper T-cell activity. On the other hand, when C3H/He mice were immunized with viable syngeneic X-5563 plasmacytoma or MM102 mammary tumour cells, the former generated preferentially killer T-cell activity, whereas the latter induced predominantly helper T-cell activity against tumour-associated transplantation antigens. Thus, immunization with transplantation antigen(s) does not always induce both helper and killer T-cell activities in parallel, but a certain antigenic system induces predominantly one type of T-cell response, thus indicating that two distinct subsets of helper and killer T cells against the transplantation antigen(s) can be raised independently without an absolute requirement of collaboration between these different T-cells subsets."}
{"id": "PMID:1085284", "title": "Helper T-cell kinetics and investigation of antigen receptor expression on early and memory T-helper cells.", "content": "The subcutaneous application of soluble antigen resulted in generation of helper T lymphocytes in the draining lymph nodes, tested 4 days after priming in the adoptive transfer system. Four days later a specific helper T-cell population was detected in the spleen. B-cell activity could be demonstrated 4-5 days after helper T-cell activity in both organs. We investigated the early helper T-cell population and memory T cells with respect to differences in the expression of antigen receptors. No such differences could be detected, either by anti-immunoglobulin-coated Degalan columns or by an antigen-specific rosetting method.", "contents": "Helper T-cell kinetics and investigation of antigen receptor expression on early and memory T-helper cells. The subcutaneous application of soluble antigen resulted in generation of helper T lymphocytes in the draining lymph nodes, tested 4 days after priming in the adoptive transfer system. Four days later a specific helper T-cell population was detected in the spleen. B-cell activity could be demonstrated 4-5 days after helper T-cell activity in both organs. We investigated the early helper T-cell population and memory T cells with respect to differences in the expression of antigen receptors. No such differences could be detected, either by anti-immunoglobulin-coated Degalan columns or by an antigen-specific rosetting method."}
{"id": "PMID:1085285", "title": "Synergy between human T and B lymphocytes in their response to phythaemagglutinin and pokeweed mitogen.", "content": "Human B- and T-lymphocyte preparations were isolated by separating T lymphocytes that formed rosettes with sheep erythrocytes from unrosetted B lymphocytes. Pokeweed mitogen stimulates the proliferation of both B- and T-lymphocyte preparations. In contrast, phytohaemagglutinin stimulates little or no proliferation of purified B lymphocytes although it stimulates the proliferation of T lymphocytes. Lymphoid preparations containing both T and B lymphocytes are more responsive to both mitogens than are either T- or B-lymphocyte preparations. This observation suggested synergy between T and B lymphocytes in the response of unfractionated lymphocytes to mitogens. The basis for this synergy was shown to be the capacity of T lymphocytes to facilitate the proliferation of B lymphocytes cultured with pokeweed mitogen or phytohaemagglutinin. The activity of T lymphocytes is not dependent upon their proliferation or attributable to their release of mitogenic factors. With regard to the clinical evaluation of immune function, our results indicate that the proliferative response of human lymphocytes to phytohaemagglutinin or pokeweed mitogen cannot be directly related to the percentage of T lymphocytes in the lymphoid preparation.", "contents": "Synergy between human T and B lymphocytes in their response to phythaemagglutinin and pokeweed mitogen. Human B- and T-lymphocyte preparations were isolated by separating T lymphocytes that formed rosettes with sheep erythrocytes from unrosetted B lymphocytes. Pokeweed mitogen stimulates the proliferation of both B- and T-lymphocyte preparations. In contrast, phytohaemagglutinin stimulates little or no proliferation of purified B lymphocytes although it stimulates the proliferation of T lymphocytes. Lymphoid preparations containing both T and B lymphocytes are more responsive to both mitogens than are either T- or B-lymphocyte preparations. This observation suggested synergy between T and B lymphocytes in the response of unfractionated lymphocytes to mitogens. The basis for this synergy was shown to be the capacity of T lymphocytes to facilitate the proliferation of B lymphocytes cultured with pokeweed mitogen or phytohaemagglutinin. The activity of T lymphocytes is not dependent upon their proliferation or attributable to their release of mitogenic factors. With regard to the clinical evaluation of immune function, our results indicate that the proliferative response of human lymphocytes to phytohaemagglutinin or pokeweed mitogen cannot be directly related to the percentage of T lymphocytes in the lymphoid preparation."}
{"id": "PMID:1085288", "title": "Interactions of murine leukemia virus (MuLV) with isolated lymphocytes. II. Infections of B and T cells with Friend virus complex indiffusion chambers and in vitro: effect of polyclonal mitogens.", "content": "The infection of isolated B and T cells by a murine leukemia virus (Friend) MuLV-F) was studied both in vitro and in vivo with an implanted diffusion chamber system. Lymphocytes were obtained from pools of normal spleen cells by filtration of the cell suspension through a nylon-wool column. The purity for both Ig positive and theta-positive cells varied between 85% and 90% in the B-cell and T-cell fractions; both lymphocyte fractions responded very well to stimulation with their respective specific polyclonal mitogens, bacterial lipopolysaccharide (LPS) and concanavalin A (Con A). Lymphocytes were infected by incubating pelleted cells in 2-6 x 10(4) FFU MuLV for 1 h at 4 degrees C and were then cultured for 5-10 days. Cells releasing infectious MuLV were enumerated as infectious centers (IC). IC were really detectable in the cultures of infected B-cells but none were found in the T-cell cultures. Addition of LPS to the culture medium increased the number of IC in B-cell fractions up to 1,000-fold. Furthermore, in T-cell cultures with LPS, IC also appeared in number which approximately correlated with the contaminating Ig+ cells of the T-cell fraction. In contrast, Con A had no consistent effect on the infection of either B or T cells. In the absence of MuLV-F, mitogenic stimulation alone did not elicit any endogenous IC. In subsequent experiments, purified lymphocytes were infected in diffusion chambers in vivo. The number of IC in infected B cells increased 1,000-fold as compared to infection in tissue culture. The peak of infection at 10 days was followed by a slight decline. Infected cells were also found in diffusion chambers containing T-cell fractions; these IC had very similar kinetics to those in B-cell-containing chambers, but their number was 10 times lower, suggesting that the infected cells were B cells, which comprised about 10% of the T-cell fraction. The virus-related antigens were detectable by immunofluorescence on the membrane of cells recovered from B-cell-bearing chambers but not on cells from T-cell-bearing chambers.", "contents": "Interactions of murine leukemia virus (MuLV) with isolated lymphocytes. II. Infections of B and T cells with Friend virus complex indiffusion chambers and in vitro: effect of polyclonal mitogens. The infection of isolated B and T cells by a murine leukemia virus (Friend) MuLV-F) was studied both in vitro and in vivo with an implanted diffusion chamber system. Lymphocytes were obtained from pools of normal spleen cells by filtration of the cell suspension through a nylon-wool column. The purity for both Ig positive and theta-positive cells varied between 85% and 90% in the B-cell and T-cell fractions; both lymphocyte fractions responded very well to stimulation with their respective specific polyclonal mitogens, bacterial lipopolysaccharide (LPS) and concanavalin A (Con A). Lymphocytes were infected by incubating pelleted cells in 2-6 x 10(4) FFU MuLV for 1 h at 4 degrees C and were then cultured for 5-10 days. Cells releasing infectious MuLV were enumerated as infectious centers (IC). IC were really detectable in the cultures of infected B-cells but none were found in the T-cell cultures. Addition of LPS to the culture medium increased the number of IC in B-cell fractions up to 1,000-fold. Furthermore, in T-cell cultures with LPS, IC also appeared in number which approximately correlated with the contaminating Ig+ cells of the T-cell fraction. In contrast, Con A had no consistent effect on the infection of either B or T cells. In the absence of MuLV-F, mitogenic stimulation alone did not elicit any endogenous IC. In subsequent experiments, purified lymphocytes were infected in diffusion chambers in vivo. The number of IC in infected B cells increased 1,000-fold as compared to infection in tissue culture. The peak of infection at 10 days was followed by a slight decline. Infected cells were also found in diffusion chambers containing T-cell fractions; these IC had very similar kinetics to those in B-cell-containing chambers, but their number was 10 times lower, suggesting that the infected cells were B cells, which comprised about 10% of the T-cell fraction. The virus-related antigens were detectable by immunofluorescence on the membrane of cells recovered from B-cell-bearing chambers but not on cells from T-cell-bearing chambers."}
{"id": "PMID:1085289", "title": "Inflammatory cells in solid murine neoplasms. II. Cell types found throughout the course of Moloney sarcoma regression or progression.", "content": "Regressing and progressing Moloney sarcomas, induced in BALB/c mice by the injection of cultured sarcoma cells (MSC)1, were sampled for histologic analysis and then disaggregated using mixtures of trypsin, collagenase and DNAse or collagenase and DNAse alone. The types of inflammatory cells (IC) found in resultant cell suspensions were determined 6, 11, 14 and 18 days post inoculation. Inflammatory infiltrates were composed almost exclusively of three cell types; neutrophils, T lymphocytes and macrophages. The extent to which each was found in tumors was related to the time post inoculation. Neutrophils were part of an early acute inflammatory response seen in both developing regressing and progressing sarcomas. The onset of regression was associated histologically with the appearance within tumors of a mononuclear inflammatory infiltrate. T lymphocytes and macrophages were the principal constituents. A higher percentage of T lymphocytes was recovered at all sampling times from regressing, compared to progressing, sarcomas. During development of the mononuclear inflammatory infiltrate there were relatively more large T cells in regressing, than in progressing tumors, and the percentage of macrophages was higher. Thereafter, the proportion of macrophages in the recovered cell population was approximately the same for both types of tumor. Such equality was more apparent than real, however, since IC were restricted to the peripheries of progressing sarcomas after the acute inflammatory phase, but continued to be found throughout regressing neoplasms. The effective ratio of macrophages and T lymphocytes to tumor cells therefore was much lower in progressing sarcomas than was suggested by percentage figures. The data presented support the concept that T lymphocytes are instrumental in causing the regression of Moloney sarcomas, possibly through interactions with macrophages.", "contents": "Inflammatory cells in solid murine neoplasms. II. Cell types found throughout the course of Moloney sarcoma regression or progression. Regressing and progressing Moloney sarcomas, induced in BALB/c mice by the injection of cultured sarcoma cells (MSC)1, were sampled for histologic analysis and then disaggregated using mixtures of trypsin, collagenase and DNAse or collagenase and DNAse alone. The types of inflammatory cells (IC) found in resultant cell suspensions were determined 6, 11, 14 and 18 days post inoculation. Inflammatory infiltrates were composed almost exclusively of three cell types; neutrophils, T lymphocytes and macrophages. The extent to which each was found in tumors was related to the time post inoculation. Neutrophils were part of an early acute inflammatory response seen in both developing regressing and progressing sarcomas. The onset of regression was associated histologically with the appearance within tumors of a mononuclear inflammatory infiltrate. T lymphocytes and macrophages were the principal constituents. A higher percentage of T lymphocytes was recovered at all sampling times from regressing, compared to progressing, sarcomas. During development of the mononuclear inflammatory infiltrate there were relatively more large T cells in regressing, than in progressing tumors, and the percentage of macrophages was higher. Thereafter, the proportion of macrophages in the recovered cell population was approximately the same for both types of tumor. Such equality was more apparent than real, however, since IC were restricted to the peripheries of progressing sarcomas after the acute inflammatory phase, but continued to be found throughout regressing neoplasms. The effective ratio of macrophages and T lymphocytes to tumor cells therefore was much lower in progressing sarcomas than was suggested by percentage figures. The data presented support the concept that T lymphocytes are instrumental in causing the regression of Moloney sarcomas, possibly through interactions with macrophages."}
{"id": "PMID:1085291", "title": "Ocular pathology induced by the suckling mouse cataract agent.", "content": "The eye disease caused by SMCA may be best characterized as an endophthalmitis, with early retinitis followed by subsequent posterior uveitis. Pathologic features of lens included: proliferation and abnormal posterior extension of lens epithelium, increased accumulation of lens capsule material, and production of aberrant lens substance. Cataractous change appears to be secondary to intraocular inflammation. In addition to the above pattern, in roughly 20 per cent of all cases, 3 weeks after inoculation, lens capsule is destroyed giving rise to a foreign body granulomatous reaction.", "contents": "Ocular pathology induced by the suckling mouse cataract agent. The eye disease caused by SMCA may be best characterized as an endophthalmitis, with early retinitis followed by subsequent posterior uveitis. Pathologic features of lens included: proliferation and abnormal posterior extension of lens epithelium, increased accumulation of lens capsule material, and production of aberrant lens substance. Cataractous change appears to be secondary to intraocular inflammation. In addition to the above pattern, in roughly 20 per cent of all cases, 3 weeks after inoculation, lens capsule is destroyed giving rise to a foreign body granulomatous reaction."}
{"id": "PMID:1085292", "title": "Improved voiding in response to electrical stimulation of the spinal cord of paraplegic dogs.", "content": "Bipolar electrodes were inserted into the conus medullaris and attached to the proximal urethra of paraplegic dogs. Repeated stimulus trains were delivered to the spinal cord electrodes and the urethral electrodes in an attempt to improve voiding induced by cord stimulation. The results indicate that the increased outflow resistance which often accompanies electromicturition can be significantly overcome both by repeated spinal cord stimulation and by prestimulation of the proximal urethra.", "contents": "Improved voiding in response to electrical stimulation of the spinal cord of paraplegic dogs. Bipolar electrodes were inserted into the conus medullaris and attached to the proximal urethra of paraplegic dogs. Repeated stimulus trains were delivered to the spinal cord electrodes and the urethral electrodes in an attempt to improve voiding induced by cord stimulation. The results indicate that the increased outflow resistance which often accompanies electromicturition can be significantly overcome both by repeated spinal cord stimulation and by prestimulation of the proximal urethra."}
{"id": "PMID:1085293", "title": "[Chewing pads, a variant of knuckle pads].", "content": "Three young male patients with chewing pads are reported. Their tic-like habit of chewing on the dorsal aspects over the middle joints of fingers I-IV caused a pad-like thickening of soft tissue. These chewing pads are a variant of \"false\" knuckle pads. The importance of a medical history and dermatological inspection is stressed to avoid unnecessary diagnostic procedures. This harmless augmentation of soft tissue is commonly confused with rheumatoid disease of the fingers, sarcoidosis or pachydermoperiostosis. Patients should be informed of the true nature of their pads, as discontinuation of their chewing habit will resolve their problems.", "contents": "[Chewing pads, a variant of knuckle pads]. Three young male patients with chewing pads are reported. Their tic-like habit of chewing on the dorsal aspects over the middle joints of fingers I-IV caused a pad-like thickening of soft tissue. These chewing pads are a variant of \"false\" knuckle pads. The importance of a medical history and dermatological inspection is stressed to avoid unnecessary diagnostic procedures. This harmless augmentation of soft tissue is commonly confused with rheumatoid disease of the fingers, sarcoidosis or pachydermoperiostosis. Patients should be informed of the true nature of their pads, as discontinuation of their chewing habit will resolve their problems."}
{"id": "PMID:1085296", "title": "Millisecond fading and recovery phenomena in fluorescent biological objects.", "content": "Cytofluorometric signals derived from some frequently used fluorophores were studied during illumination times in the millisecond range. These rapid signals were recorded on a storage oscilloscope. The objects studied included (1) Berberine sulphate stained mast cell heparin, (2) Acriflavine-Feulgen stained DNA, (3) Acridine orange stained mast cell heparin, (4) Acridine orange stained DNA and (5) Fluorescein isothiocyanate-conjugated IgG in an antinuclear factor test. A new rapid fading phenomenon, appearing as an initial peak upon the familiar slowly declining fluorescence signal, is reported. This fading, which had a duration of about 10 ms, also showed a very rapid recovery. The influence of this phenomenon on fluorometric measurement techniques is discussed. The millisecond fading phenomenon occurred in all the fluorophores studied except Fluorescein isothiocyanate-conjugated IgG. In the case of acridine orange the phenomenon was present when the dye was bound to nuclear DNA but absent when the dye was bound to mast cell heparin. This suggests that the millisecond fading and recovery phenomenon may be used in fluorescent microprobe studies.", "contents": "Millisecond fading and recovery phenomena in fluorescent biological objects. Cytofluorometric signals derived from some frequently used fluorophores were studied during illumination times in the millisecond range. These rapid signals were recorded on a storage oscilloscope. The objects studied included (1) Berberine sulphate stained mast cell heparin, (2) Acriflavine-Feulgen stained DNA, (3) Acridine orange stained mast cell heparin, (4) Acridine orange stained DNA and (5) Fluorescein isothiocyanate-conjugated IgG in an antinuclear factor test. A new rapid fading phenomenon, appearing as an initial peak upon the familiar slowly declining fluorescence signal, is reported. This fading, which had a duration of about 10 ms, also showed a very rapid recovery. The influence of this phenomenon on fluorometric measurement techniques is discussed. The millisecond fading phenomenon occurred in all the fluorophores studied except Fluorescein isothiocyanate-conjugated IgG. In the case of acridine orange the phenomenon was present when the dye was bound to nuclear DNA but absent when the dye was bound to mast cell heparin. This suggests that the millisecond fading and recovery phenomenon may be used in fluorescent microprobe studies."}
{"id": "PMID:1085297", "title": "Histochemical characterization of the monoamine-containing cells of the adenohypophysis in the Chinese quail.", "content": "The demonstration of biogenic amine-containing cell is classically performed by means of the formaldehyde-induced fluorescence technique after administration of the monoamine precursors, L-dopa or 5-hydroxytryptophan (5-HTP. The injection of these two two compounds rapidly leads to the formation of large amounts. The biochemical pathway evoked above   is studied in the avian adenohypophysis by two different methods involving enzyme...", "contents": "Histochemical characterization of the monoamine-containing cells of the adenohypophysis in the Chinese quail. The demonstration of biogenic amine-containing cell is classically performed by means of the formaldehyde-induced fluorescence technique after administration of the monoamine precursors, L-dopa or 5-hydroxytryptophan (5-HTP. The injection of these two two compounds rapidly leads to the formation of large amounts. The biochemical pathway evoked above   is studied in the avian adenohypophysis by two different methods involving enzyme..."}
{"id": "PMID:1085298", "title": "Electrophysiological evidence for a topographical projection of the nasal mucosa onto the olfactory bulb of the frog.", "content": "Three olfactory nerve branches respectively subserving either a medial, an intermediate, or a lateral region of the dorsal olfactory receptor sheet of the bullfrog Rana catesbeiana were electrically stimulated with bipolar platinum hook electrodes. Extracellular single unit responses from 93 second-order cells in different regions of the olfactory bulb were recorded with metal-filled glass micropipets. The excitatory responsiveness of each unit to the stimulation of each of the three nerve branches (response profile) was determined. Some units were sensitive to stimulation of each of the three nerve branches, thus suggesting a wide projection from the entire receptor sheet. On the other hand, other units were more selective. Of this latter group, units in the lateral bulb were excited by nerve branches subserving the more lateral regions of the receptor sheet; units in the medial bulb were excited by the nerve branches subserving the more medial regions of the receptor sheet. These data provide electrophysiological evidence for a topographical projection of the olfactory receptor sheet onto the olfactory bulb, and further suggest that the projections onto different bulbar cells vary in degree of localization.", "contents": "Electrophysiological evidence for a topographical projection of the nasal mucosa onto the olfactory bulb of the frog. Three olfactory nerve branches respectively subserving either a medial, an intermediate, or a lateral region of the dorsal olfactory receptor sheet of the bullfrog Rana catesbeiana were electrically stimulated with bipolar platinum hook electrodes. Extracellular single unit responses from 93 second-order cells in different regions of the olfactory bulb were recorded with metal-filled glass micropipets. The excitatory responsiveness of each unit to the stimulation of each of the three nerve branches (response profile) was determined. Some units were sensitive to stimulation of each of the three nerve branches, thus suggesting a wide projection from the entire receptor sheet. On the other hand, other units were more selective. Of this latter group, units in the lateral bulb were excited by nerve branches subserving the more lateral regions of the receptor sheet; units in the medial bulb were excited by the nerve branches subserving the more medial regions of the receptor sheet. These data provide electrophysiological evidence for a topographical projection of the olfactory receptor sheet onto the olfactory bulb, and further suggest that the projections onto different bulbar cells vary in degree of localization."}
{"id": "PMID:1085299", "title": "Biological properties of a Haemophilus influenzae restriction enzyme, Hind I.", "content": "A type I restriction enzyme from Haemophilus influenzae, Hind I, which requires adenosine 5' -triphosphate and 5-adenosyl methionine, was studied for its activity on transfecting and transforming deoxyribonculeic acid (DNA). The enzyme reduced the size of unmodified bacteriophage S2 DNA from 37 X 10(6) daltons to approximately 10 X 10(6) daltons, but did not affect modified S2 DNA. Unmodified transforming DNA was attacked in vitro by Hind I; however, relatively low levels of inactivation were obtained for single markers, and linked transformants were inactivated as a function of the distance between markers. In contrast, unmodified bacterial DNA was not inactivated in vivo for either single or linked markers by the Hind I restriction system, probably because the segments generated by Hind I were still capable of being integrated in vivo. The lack of preferential inactivation of markers by the enzyme suggests that it makes random breaks in the DNA.", "contents": "Biological properties of a Haemophilus influenzae restriction enzyme, Hind I. A type I restriction enzyme from Haemophilus influenzae, Hind I, which requires adenosine 5' -triphosphate and 5-adenosyl methionine, was studied for its activity on transfecting and transforming deoxyribonculeic acid (DNA). The enzyme reduced the size of unmodified bacteriophage S2 DNA from 37 X 10(6) daltons to approximately 10 X 10(6) daltons, but did not affect modified S2 DNA. Unmodified transforming DNA was attacked in vitro by Hind I; however, relatively low levels of inactivation were obtained for single markers, and linked transformants were inactivated as a function of the distance between markers. In contrast, unmodified bacterial DNA was not inactivated in vivo for either single or linked markers by the Hind I restriction system, probably because the segments generated by Hind I were still capable of being integrated in vivo. The lack of preferential inactivation of markers by the enzyme suggests that it makes random breaks in the DNA."}
{"id": "PMID:1085300", "title": "Age and activity effects on rate of bone mineral loss.", "content": "It has been postulated that the rate of mineral loss in postmenopausal women remains constant with aging and that the decreased activities of daily living associated with aging contribute to mineral loss. These hypothese were examined by measuring the bone mineral content at the midshaft of the radius with the photon absorption technique. The estimated rate of loss was calculated in a cross-sectional study as the regression coefficient of bone mineral content vs. age and in a longitudinal study as the regression coefficient of bone mineral content vs. time. In the cross-sectional study, Group A, which consisted of 264 women aged 50-72 yr, had an estimated rate of loss of -0.0114 +/- 0.0014 (SE) g/cm per yr. Group B, which consisted of 266 women aged 73-96 yr, had an estimated rate of loss of -0.0055 +/- 0.0017 g/cm per yr. In the longitudinal study, Group C consisted of 33 women aged 51-65 yr who were followed for an average of 4.5 yr with a mean number of 16 visits per subject; they were found to have a mean rate of loss of -0.00990 +/- 0.00107 g/cm per yr. Group D consisted of 38 women aged 70-91 yr who were followed for an average of 3.8 yr with a mean number of 31 visits per subjects; they were found to have a mean rate of loss of -0.00020 +/- 0.00236 g/cm per yr. The estimated and directly measured rates of loss were more rapid in the younger groups than in the older groups (A vs. B, P less than 0.001; C vs. D, P less than 0.001). These data demonstrate that the mean rate of mineral loss is not constant with aging and that in elderly subjects it is significantly slower than that of the earlier postmenopausal years. Since the elderly women were the less active, these findings suggest that factors other than decreased physical activity are more important in determining the rates of mineral loss.", "contents": "Age and activity effects on rate of bone mineral loss. It has been postulated that the rate of mineral loss in postmenopausal women remains constant with aging and that the decreased activities of daily living associated with aging contribute to mineral loss. These hypothese were examined by measuring the bone mineral content at the midshaft of the radius with the photon absorption technique. The estimated rate of loss was calculated in a cross-sectional study as the regression coefficient of bone mineral content vs. age and in a longitudinal study as the regression coefficient of bone mineral content vs. time. In the cross-sectional study, Group A, which consisted of 264 women aged 50-72 yr, had an estimated rate of loss of -0.0114 +/- 0.0014 (SE) g/cm per yr. Group B, which consisted of 266 women aged 73-96 yr, had an estimated rate of loss of -0.0055 +/- 0.0017 g/cm per yr. In the longitudinal study, Group C consisted of 33 women aged 51-65 yr who were followed for an average of 4.5 yr with a mean number of 16 visits per subject; they were found to have a mean rate of loss of -0.00990 +/- 0.00107 g/cm per yr. Group D consisted of 38 women aged 70-91 yr who were followed for an average of 3.8 yr with a mean number of 31 visits per subjects; they were found to have a mean rate of loss of -0.00020 +/- 0.00236 g/cm per yr. The estimated and directly measured rates of loss were more rapid in the younger groups than in the older groups (A vs. B, P less than 0.001; C vs. D, P less than 0.001). These data demonstrate that the mean rate of mineral loss is not constant with aging and that in elderly subjects it is significantly slower than that of the earlier postmenopausal years. Since the elderly women were the less active, these findings suggest that factors other than decreased physical activity are more important in determining the rates of mineral loss."}
{"id": "PMID:1085301", "title": "Use of a problem-oriented dental record in undergraduate dental education.", "content": "This paper describes how the concepts and methods of problem-orientation have been applied in the clinical education of dental students. It makes the point that the problem-oriented dental record is only one part of a complete system which has five interrelated components. The problem-oriented dental record is discussed in detail, and the paper includes examples of all four sections of the record. All sections of the record are interrelated, and the steps in identifying and resolving problems follow a logical sequence: (1) collect the data base, (2) write the cue list, (3) write the problem list, (4) write the initial plan, (5) write the final plan, and (6) record progress. The problem-oriented dental record is seen as a tool for patient care, audit, and education. The main advantage lies in the way information is organized and recorded. The record is highly communicative and permits everyone involved an opportunity to relate the data collected to the problem list and to the decisions made regarding how problems will be resolved. The record and the systems were borrowed from Weed and are being adapted for use in dentistry. Although still in the trial stages, this system has proven to have many more advantages than other record-keeping systems and is invaluable as a tool for education. In the future we plan to use computers to help store and retrieve data from these records. We anticipate that a dental education built on this record will have a lasting impact on the graduates' approach to patient care. This should result in a graduate who is conversant with medical colleagues and systematic in the collection of data, listing of problems, and resolution of patient problems. Finally, we believe that the problem-oriented dental record is also a good tool to help the dental graduate become more empathetic with patients and more aware of their total health needs. The record provides a mechanism for recording physical and emotional information as well as the oral status.", "contents": "Use of a problem-oriented dental record in undergraduate dental education. This paper describes how the concepts and methods of problem-orientation have been applied in the clinical education of dental students. It makes the point that the problem-oriented dental record is only one part of a complete system which has five interrelated components. The problem-oriented dental record is discussed in detail, and the paper includes examples of all four sections of the record. All sections of the record are interrelated, and the steps in identifying and resolving problems follow a logical sequence: (1) collect the data base, (2) write the cue list, (3) write the problem list, (4) write the initial plan, (5) write the final plan, and (6) record progress. The problem-oriented dental record is seen as a tool for patient care, audit, and education. The main advantage lies in the way information is organized and recorded. The record is highly communicative and permits everyone involved an opportunity to relate the data collected to the problem list and to the decisions made regarding how problems will be resolved. The record and the systems were borrowed from Weed and are being adapted for use in dentistry. Although still in the trial stages, this system has proven to have many more advantages than other record-keeping systems and is invaluable as a tool for education. In the future we plan to use computers to help store and retrieve data from these records. We anticipate that a dental education built on this record will have a lasting impact on the graduates' approach to patient care. This should result in a graduate who is conversant with medical colleagues and systematic in the collection of data, listing of problems, and resolution of patient problems. Finally, we believe that the problem-oriented dental record is also a good tool to help the dental graduate become more empathetic with patients and more aware of their total health needs. The record provides a mechanism for recording physical and emotional information as well as the oral status."}
{"id": "PMID:1085306", "title": "[Pathogenic action and sero-immunologic phenomena of Trichomonas vaginalis].", "content": "Trichomonas vaginalis is a frequent parasite of the urogenital system of the human been. The pathological manifestations are different with each strain. These variations of the pathogenic action of the parasite are found in the study of the experimental pathogenic action of the animal and in vitro on cell culture. The sero-immunological phenomens which lead to the presence of the parasite are not completely known. Some technics was proposed for the diagnosis. Their practical utilisation is not completely related to the heterogenicity of the antigenic structure of the different strains of Trichomonas vaginalis.", "contents": "[Pathogenic action and sero-immunologic phenomena of Trichomonas vaginalis]. Trichomonas vaginalis is a frequent parasite of the urogenital system of the human been. The pathological manifestations are different with each strain. These variations of the pathogenic action of the parasite are found in the study of the experimental pathogenic action of the animal and in vitro on cell culture. The sero-immunological phenomens which lead to the presence of the parasite are not completely known. Some technics was proposed for the diagnosis. Their practical utilisation is not completely related to the heterogenicity of the antigenic structure of the different strains of Trichomonas vaginalis."}
{"id": "PMID:1085307", "title": "[Consumption coagulopathies and severe postpartum hemorrhage].", "content": "15 patients were referred to the Intensive Care Unit immediately after delivery for severe post-partum haemorrhage. In 7 cases complications followed retroplacental haemotoma formation and in the other 8 cases they were due to secondary haemorrhages from the placental site and/or from tears of the cervix or vagina. It is a clinical picture that is associated with a state of shock, with continuing massive bleeding associated with failure of the blood to clot and which persists and becomes worse with the transfusion of stored blood and of the coagulation factors. In all these cases severe coagulation pathology due to consumption defects is revealed. We must point out that tears of the cervix and vagina which have been neglected and failure to compensate for the original blood loss are among the aetiological factors. Treatment with Heparin giving a dose of 1 to 3 mg per kg of body weight per 24 hours and controlled by a strict biological check together with symptomatic resuscitation from the state of shock and from the coagulation factors is successful (9 cases) if it is undertaken early on. A fatal outcome, whether it is due to a persistent state of shock (1 case) or to visceral complications of diffuse intravascular coagulation (C.I.V.D.) (5 cases) can be found where treatment is delayed and when insufficient blood is replaced and when antifibrinolytics are prescribed. A preventive therapeutic plan as well as a curative one can be drawn up for these cases of obstetrical drama.", "contents": "[Consumption coagulopathies and severe postpartum hemorrhage]. 15 patients were referred to the Intensive Care Unit immediately after delivery for severe post-partum haemorrhage. In 7 cases complications followed retroplacental haemotoma formation and in the other 8 cases they were due to secondary haemorrhages from the placental site and/or from tears of the cervix or vagina. It is a clinical picture that is associated with a state of shock, with continuing massive bleeding associated with failure of the blood to clot and which persists and becomes worse with the transfusion of stored blood and of the coagulation factors. In all these cases severe coagulation pathology due to consumption defects is revealed. We must point out that tears of the cervix and vagina which have been neglected and failure to compensate for the original blood loss are among the aetiological factors. Treatment with Heparin giving a dose of 1 to 3 mg per kg of body weight per 24 hours and controlled by a strict biological check together with symptomatic resuscitation from the state of shock and from the coagulation factors is successful (9 cases) if it is undertaken early on. A fatal outcome, whether it is due to a persistent state of shock (1 case) or to visceral complications of diffuse intravascular coagulation (C.I.V.D.) (5 cases) can be found where treatment is delayed and when insufficient blood is replaced and when antifibrinolytics are prescribed. A preventive therapeutic plan as well as a curative one can be drawn up for these cases of obstetrical drama."}
{"id": "PMID:1085308", "title": "Cutaneous basophil hypersensitivity uncovered in the cell transfer of classical tuberculin hypersensitivity.", "content": "Cellular transfer of cutaneous basophil hypersensitivity (CBH) was studied. Guinea pigs immunized for CBH with incomplete Freund's adjuvant (IFA) provided cells which could transfer delayed and basophil-rich reactions in skin tests of recipients. Guinea pigs immunized with complete classical tuberculin-type delayed hypersensitivity reactions (DH), which are characteristically devoid of basophils. However, recipients of cells from donors with DH, surprisingly, were found to have delayed skin reactions containing large basophil infiltrates which were lacking in the donors. Thus, recipients of classical cell transfers of tuberculin-type DH had delayed reactions which resembled CBH. Control experiments verified that the cell transfer of CBH from donors with DH was due to passive transfer with live cells and not transfer of contaminating humoral factors or active sensitization of recipients. It was concluded that cutaneous basophil responses were suppressed in CFA-immunized donors and expressed in cell transfer recipients. Cells from donors immunized with CFA were enriched for nonadherent and nonimmunoglobulin-bearing lymphocytes by passage through nylon wool columns, and these cells transferred conjugate specific CBH reactions. It was concluded that cells mediating these transfers were probably T cells. The finding of basophils in cell transfers of DH and a variety of other findings suggesting complex regulation of basophil numbers in tissue lead to the conclusion that the term CBH be used to simply describe a basophil-containing skin reaction.", "contents": "Cutaneous basophil hypersensitivity uncovered in the cell transfer of classical tuberculin hypersensitivity. Cellular transfer of cutaneous basophil hypersensitivity (CBH) was studied. Guinea pigs immunized for CBH with incomplete Freund's adjuvant (IFA) provided cells which could transfer delayed and basophil-rich reactions in skin tests of recipients. Guinea pigs immunized with complete classical tuberculin-type delayed hypersensitivity reactions (DH), which are characteristically devoid of basophils. However, recipients of cells from donors with DH, surprisingly, were found to have delayed skin reactions containing large basophil infiltrates which were lacking in the donors. Thus, recipients of classical cell transfers of tuberculin-type DH had delayed reactions which resembled CBH. Control experiments verified that the cell transfer of CBH from donors with DH was due to passive transfer with live cells and not transfer of contaminating humoral factors or active sensitization of recipients. It was concluded that cutaneous basophil responses were suppressed in CFA-immunized donors and expressed in cell transfer recipients. Cells from donors immunized with CFA were enriched for nonadherent and nonimmunoglobulin-bearing lymphocytes by passage through nylon wool columns, and these cells transferred conjugate specific CBH reactions. It was concluded that cells mediating these transfers were probably T cells. The finding of basophils in cell transfers of DH and a variety of other findings suggesting complex regulation of basophil numbers in tissue lead to the conclusion that the term CBH be used to simply describe a basophil-containing skin reaction."}
{"id": "PMID:1085309", "title": "Thymic function in NZB mice. II. Regulatory influence of a circulating thymic factor on antibody production against polyvinylpyrrolidone in NZB mice.", "content": "Administration of a circulating thymic factor isolated from normal pig blood prevented the development of the exaggerated production of anti-polyvinyl pyrrolidone (PVP) antibody in young NZB mice. However, treatment was ineffective if initiated after the 4th week of life at a time when endogenous serum thymic factor (TF) normally disappears in these mice. These data suggest that circulating TF is necessary for the survival of short-lived suppressor T cells normally implicated in the regulation of the production of antibodies against PVP, a thymus-independent antigen. In older NZB mice, TF treatment increased paradoxically anti-PVP antibody production, which suggests that \"amplifier\" T cell activity could also be under TF influence.", "contents": "Thymic function in NZB mice. II. Regulatory influence of a circulating thymic factor on antibody production against polyvinylpyrrolidone in NZB mice. Administration of a circulating thymic factor isolated from normal pig blood prevented the development of the exaggerated production of anti-polyvinyl pyrrolidone (PVP) antibody in young NZB mice. However, treatment was ineffective if initiated after the 4th week of life at a time when endogenous serum thymic factor (TF) normally disappears in these mice. These data suggest that circulating TF is necessary for the survival of short-lived suppressor T cells normally implicated in the regulation of the production of antibodies against PVP, a thymus-independent antigen. In older NZB mice, TF treatment increased paradoxically anti-PVP antibody production, which suggests that \"amplifier\" T cell activity could also be under TF influence."}
{"id": "PMID:1085310", "title": "Influence of H-2 on the ontogenesis of a spleen cell population which lacks C3 receptors and Thy-1 antigen.", "content": "During ontogeny there are marked differences in the cellularity of the spleen of mice from different inbred strains. Data from segregation analysis demonstrate that one of the genes involved in the control of the size of the spleen at 15 days lies in the H-2 region. In contrast to previous reports, we find that the cell population under H-2 influence lacks complement receptors.", "contents": "Influence of H-2 on the ontogenesis of a spleen cell population which lacks C3 receptors and Thy-1 antigen. During ontogeny there are marked differences in the cellularity of the spleen of mice from different inbred strains. Data from segregation analysis demonstrate that one of the genes involved in the control of the size of the spleen at 15 days lies in the H-2 region. In contrast to previous reports, we find that the cell population under H-2 influence lacks complement receptors."}
{"id": "PMID:1085311", "title": "Tolerance and contact sensitivity to DNFB in mice. VI. Inhibition of afferent sensitivity by suppressor T cells in adoptive tolerance.", "content": "Tolerance in contact sensitivity to DNFB can be adoptively transferred to normal mice with lymph node cells from tolerant donors. This tolerance is antigen specific and is mediated by T cells, i.e., \"suppressor\" T cells. Experiments were carried out to investigate the mechanism(s) by which the suppressor T cells induce tolerance to DNFB contact sensitivity. The suppressor cells were effective only if they were present during the early stages of the afferent limb of sensitization. As measured by DNA synthesis, cell proliferation in the draining lymph nodes of recipients of suppressor cells was found to be significantly less than in control animals indicating that the suppressor cells acted, at least in part, by limiting or inhibiting DNFB-induced cell proliferation. This inhibition was shown to be antigen specific since the DNFB suppressor cells did not inhibit cell proliferation induced by oxazolone, an unrelated contact sensitizer. The ability to DNFB tolerant cells to block  afferent sensitization pathways differs from the mechanism of tolerance to picryl chloride, reported by others, where efferent pathways are blocked.", "contents": "Tolerance and contact sensitivity to DNFB in mice. VI. Inhibition of afferent sensitivity by suppressor T cells in adoptive tolerance. Tolerance in contact sensitivity to DNFB can be adoptively transferred to normal mice with lymph node cells from tolerant donors. This tolerance is antigen specific and is mediated by T cells, i.e., \"suppressor\" T cells. Experiments were carried out to investigate the mechanism(s) by which the suppressor T cells induce tolerance to DNFB contact sensitivity. The suppressor cells were effective only if they were present during the early stages of the afferent limb of sensitization. As measured by DNA synthesis, cell proliferation in the draining lymph nodes of recipients of suppressor cells was found to be significantly less than in control animals indicating that the suppressor cells acted, at least in part, by limiting or inhibiting DNFB-induced cell proliferation. This inhibition was shown to be antigen specific since the DNFB suppressor cells did not inhibit cell proliferation induced by oxazolone, an unrelated contact sensitizer. The ability to DNFB tolerant cells to block  afferent sensitization pathways differs from the mechanism of tolerance to picryl chloride, reported by others, where efferent pathways are blocked."}
{"id": "PMID:1085312", "title": "Immune response of mice to the Thy-1.1 antigen: intra-H-2 mapping of the complementary Ir-Thy-1 loci.", "content": "The primary immune response to the Thy-1.1 antigen was measured by a plaque assay that detected cells producing antibodies lytic for AKR thymocytes. F1 hybrids which were b/d heterozygous at the entire H-2 complex or at only some of its regions were studied. All F1 hybrids which were b/d heterozygotes at the K, IA and possibly IB regions displayed a complementary effect of the alleles at the Ir-Thy-1A and Ir-Thy-1B loci. The complementary effect was indicated by the significantly higher responses of the F1 hybrids than the responses of their parental strains. No complementary effect could be demonstrated in F1 hybrids which were b/d heterozygous at the IB, IC, S and D regions, but not at the K and IA regions. The results excluded participation of the IC, S and D regions in the phenomenon of the complementation. Furthermore, one of the Ir-Thy-1 loci could be mapped to the left of the IB subregion, most likely in the IA subregion of the H-2 complex. The second Ir-Thy-1 locus could be mapped to the left of the IC subregion in either the IA or IB subregion.", "contents": "Immune response of mice to the Thy-1.1 antigen: intra-H-2 mapping of the complementary Ir-Thy-1 loci. The primary immune response to the Thy-1.1 antigen was measured by a plaque assay that detected cells producing antibodies lytic for AKR thymocytes. F1 hybrids which were b/d heterozygous at the entire H-2 complex or at only some of its regions were studied. All F1 hybrids which were b/d heterozygotes at the K, IA and possibly IB regions displayed a complementary effect of the alleles at the Ir-Thy-1A and Ir-Thy-1B loci. The complementary effect was indicated by the significantly higher responses of the F1 hybrids than the responses of their parental strains. No complementary effect could be demonstrated in F1 hybrids which were b/d heterozygous at the IB, IC, S and D regions, but not at the K and IA regions. The results excluded participation of the IC, S and D regions in the phenomenon of the complementation. Furthermore, one of the Ir-Thy-1 loci could be mapped to the left of the IB subregion, most likely in the IA subregion of the H-2 complex. The second Ir-Thy-1 locus could be mapped to the left of the IC subregion in either the IA or IB subregion."}
{"id": "PMID:1085313", "title": "An appraisal of Fc receptors on human peripheral blood B and L lymphocytes.", "content": "Human circulating lymphocytes with easily detectable surface immunoglobulin have been divided into two populations, B cells and L cells. This second population lacks membrane-incorporated Ig, but has a receptor for membrane-labile cytophilic IgG. In this study purified B and L lymphocytes were examined for Fc receptors that bind aggregated IgG and IgG complexed to erythrocytes. Purified lymphocyte populations were prepared by nylon columns and by negative selection with rosetting techniques. L lymphocytes bound aggregated guinea pig and human IgG, and formed rosettes with human erythrocytes sensitized with Ripley IgG (EA). Treatment of L lymphocytes with trypsin had no effect on the receptors for IgG. B lymphocytes did not bind EA and attachment of aggregated IgG was variable; up to one-third of these cells fixed aggregated human IgG to the cell membrane. Trypsin treatment abolished binding of Agg-IgG to B cells in sharp contrast to its effect on L cells. Furthermore, double-label immunofluorescence studies showed that cells with both membrane-incorporated Ig and receptors for aggregated guinea pig IgG were rare. These studies indicate that human peripheral blood B lymphocytes lack a high affinity, trypsin-resistant Fc receptor that is present on L lymphocytes.", "contents": "An appraisal of Fc receptors on human peripheral blood B and L lymphocytes. Human circulating lymphocytes with easily detectable surface immunoglobulin have been divided into two populations, B cells and L cells. This second population lacks membrane-incorporated Ig, but has a receptor for membrane-labile cytophilic IgG. In this study purified B and L lymphocytes were examined for Fc receptors that bind aggregated IgG and IgG complexed to erythrocytes. Purified lymphocyte populations were prepared by nylon columns and by negative selection with rosetting techniques. L lymphocytes bound aggregated guinea pig and human IgG, and formed rosettes with human erythrocytes sensitized with Ripley IgG (EA). Treatment of L lymphocytes with trypsin had no effect on the receptors for IgG. B lymphocytes did not bind EA and attachment of aggregated IgG was variable; up to one-third of these cells fixed aggregated human IgG to the cell membrane. Trypsin treatment abolished binding of Agg-IgG to B cells in sharp contrast to its effect on L cells. Furthermore, double-label immunofluorescence studies showed that cells with both membrane-incorporated Ig and receptors for aggregated guinea pig IgG were rare. These studies indicate that human peripheral blood B lymphocytes lack a high affinity, trypsin-resistant Fc receptor that is present on L lymphocytes."}
{"id": "PMID:1085314", "title": "Replication of dengue-2 virus in cultured human lymphoblastoid cells and subpopulations of human peripheral leukocytes.", "content": "We studied the susceptibility of four human lymphoblastoid cell lines (HCL) and of subpopulations of circulating peripheral human leukocytes to dengue-2 virus infection. HCL with B cell characteristics (Raji, Wil 2WT, 8866), B-type peripheral lymphocytes, and macrophages were productively infected by dengue-2 virus. In contrast, an HCL with T cell characteristics (MOLT-4), T type peripheral lymphocytes, and polymorphonuclear (PMN) cells did not become infected and replicate dengue-2 virus. PMN cells did not adsorb dengue-2 virus, suggesting lack of viral receptors. However, T-type cultured lymphoblasts and T-type peripheral lymphocytes adsorbed dengue-2 virus, suggesting that the block in viral replication involves some stage of infection occurring after adsorption. Permissiveness of B-type HCL to dengue-2 virus infection was dependent on the virus seed used but the virus titers obtained among the susceptible HCL varied. HCL infected persistently with dengue-2 virus have been established. Human peripheral lymphocytes inoculated after cultivation for 3 days in complete medium alone or complete medium supplemented with mitogens replicated dengue-2 virus. In contrast, unstimulated peripheral lymphocytes inoculated immediately after isolation adsorbed dengue-2 but did not support its replication. Mitogen-treated and untreated macrophages replicated dengue-2 virus equally well. The efficiency of dengue-2 virus replication by macrophages was higher than that of peripheral lymphocytes but lower than that of HCL.", "contents": "Replication of dengue-2 virus in cultured human lymphoblastoid cells and subpopulations of human peripheral leukocytes. We studied the susceptibility of four human lymphoblastoid cell lines (HCL) and of subpopulations of circulating peripheral human leukocytes to dengue-2 virus infection. HCL with B cell characteristics (Raji, Wil 2WT, 8866), B-type peripheral lymphocytes, and macrophages were productively infected by dengue-2 virus. In contrast, an HCL with T cell characteristics (MOLT-4), T type peripheral lymphocytes, and polymorphonuclear (PMN) cells did not become infected and replicate dengue-2 virus. PMN cells did not adsorb dengue-2 virus, suggesting lack of viral receptors. However, T-type cultured lymphoblasts and T-type peripheral lymphocytes adsorbed dengue-2 virus, suggesting that the block in viral replication involves some stage of infection occurring after adsorption. Permissiveness of B-type HCL to dengue-2 virus infection was dependent on the virus seed used but the virus titers obtained among the susceptible HCL varied. HCL infected persistently with dengue-2 virus have been established. Human peripheral lymphocytes inoculated after cultivation for 3 days in complete medium alone or complete medium supplemented with mitogens replicated dengue-2 virus. In contrast, unstimulated peripheral lymphocytes inoculated immediately after isolation adsorbed dengue-2 but did not support its replication. Mitogen-treated and untreated macrophages replicated dengue-2 virus equally well. The efficiency of dengue-2 virus replication by macrophages was higher than that of peripheral lymphocytes but lower than that of HCL."}
{"id": "PMID:1085315", "title": "Site of action of a soluble immune response suppressor (SIRS) produced by concanavalin A-activated spleen cells.", "content": "The cellular site of action of SIRS, a soluble immune response suppressor released by Con A-activated spleen cells which suppresses antibody responses to heterologous erythrocytes by murine spleen cells in vitro, was investigated. Exposure of spleen cells to SIRS for 2 hr at 37 degrees C or 1 hr at 4 degrees C was sufficient to suppress 5-day antibody responses in vitro. Similar exposure of splenic or peritoneal exudate macrophages to SIRS also suppressed antibody responses by untreated splenic lymphoid cells; exposure of splenic lymphoid cells to SIRS was without effect. SIRS did not act via T cells which might have contaminated the macrophage preparations. SIRS-mediated suppression could be partially overcome by an excess of normal peritoneal exudate macrophages, but not by an excess of T or B cells. These data indicate that the target cell of SIRS activity is the macrophage. The results are discussed in the context of macrophage functions that could be affected by SIRS.", "contents": "Site of action of a soluble immune response suppressor (SIRS) produced by concanavalin A-activated spleen cells. The cellular site of action of SIRS, a soluble immune response suppressor released by Con A-activated spleen cells which suppresses antibody responses to heterologous erythrocytes by murine spleen cells in vitro, was investigated. Exposure of spleen cells to SIRS for 2 hr at 37 degrees C or 1 hr at 4 degrees C was sufficient to suppress 5-day antibody responses in vitro. Similar exposure of splenic or peritoneal exudate macrophages to SIRS also suppressed antibody responses by untreated splenic lymphoid cells; exposure of splenic lymphoid cells to SIRS was without effect. SIRS did not act via T cells which might have contaminated the macrophage preparations. SIRS-mediated suppression could be partially overcome by an excess of normal peritoneal exudate macrophages, but not by an excess of T or B cells. These data indicate that the target cell of SIRS activity is the macrophage. The results are discussed in the context of macrophage functions that could be affected by SIRS."}
{"id": "PMID:1085324", "title": "T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells.", "content": "Allotype suppressor T cells (Ts) generated in SJL X BALB/c mice specifically suppress production of antibodies marked with the Ig-1a allotype. The studies presented here show that allotypes Ts suppress by specifically removing helper T cell (Th) activity required to facilitate differentiation and expansion of B cells to Ig-1b antibody-forming cells. We show first that Ts and Th belong to different T-cell subclasses as defined by Ly surface antigens. Ts are Ly2+Lyl- and thus belong to the same subclass as cytotoxic precursor and effector cells; Th are Lyl+Ly2- cells and thus belong to the subclass containing cells which can exert helper functions and initiate delayed hypersensitivity reactions. Placing these cells in these two subclasses shows that Th are different from Ts and suggests that they play different roles in regulating antibody responses. The difference in these roles is defined by the evidence presented here showing that Ts attack Th and regulate the antibody response by specifically regulating the availability of Th activity. We show that in allotype suppressed mice, Ts which suppress Ig-1b antibody production have completely removed the Th activity of helping Ig-1b cells without impairing Th activity which helps other IgB B cells. These findings imply the existence of allotype-specific Th for Ig-1b cells (Ig-1b Th). We directly establish that Ig-1b cells require such help by showing that carrier-primed spleen cells from Iga/Iga congenic hybrids help Ig-1a B cells from hapten-primed Igb/Iga donors but do not help Ig-1b B cells from the same donor in the same adoptive recipient.", "contents": "T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells. Allotype suppressor T cells (Ts) generated in SJL X BALB/c mice specifically suppress production of antibodies marked with the Ig-1a allotype. The studies presented here show that allotypes Ts suppress by specifically removing helper T cell (Th) activity required to facilitate differentiation and expansion of B cells to Ig-1b antibody-forming cells. We show first that Ts and Th belong to different T-cell subclasses as defined by Ly surface antigens. Ts are Ly2+Lyl- and thus belong to the same subclass as cytotoxic precursor and effector cells; Th are Lyl+Ly2- cells and thus belong to the subclass containing cells which can exert helper functions and initiate delayed hypersensitivity reactions. Placing these cells in these two subclasses shows that Th are different from Ts and suggests that they play different roles in regulating antibody responses. The difference in these roles is defined by the evidence presented here showing that Ts attack Th and regulate the antibody response by specifically regulating the availability of Th activity. We show that in allotype suppressed mice, Ts which suppress Ig-1b antibody production have completely removed the Th activity of helping Ig-1b cells without impairing Th activity which helps other IgB B cells. These findings imply the existence of allotype-specific Th for Ig-1b cells (Ig-1b Th). We directly establish that Ig-1b cells require such help by showing that carrier-primed spleen cells from Iga/Iga congenic hybrids help Ig-1a B cells from hapten-primed Igb/Iga donors but do not help Ig-1b B cells from the same donor in the same adoptive recipient."}
{"id": "PMID:1085325", "title": "Two stages of B-cell memory development with different T-cell requirements.", "content": "We present evidence here for two stages in B-memory cell development, the first of which is T independent and the second T dependent. For these studies, we use a new type of T-deficient mouse (allotype suppressed) which specifically lacks T-helper activity (Th) for a subset of memory B cells responsible for approximately 10% of the overall IgG antibody response. We have shown elsewhere that these mice (SJL X BALB/c hybrids suppressed for Ig-1b) lack Th capable of helping Ig-1b memory cells, although they have normal Th activity for all other IgG memory B cells. This selective Th deficiency allows study of the effects of T depletion on memory development and avidity maturation of one population of B cells under conditions where the bulk of the immune response in the animal is proceeding normally, thus obviating environmental problems due to secondary effects of T depletion. With this sytem, we show that after a single priming dose of 2,4-dinitrophenyl-keyhole limpet hemocyanin, the memory B-cell pool in suppressed and nonsuppressed donors is indistinguishable with respect to magnitude and avidity of the response for all IgG antibodies produced, including Ig-1b antibody, despite the fact that expression of Ig-1b memory cells is prevented in intact Ig-1b-suppressed mice by the absence of Th capbale of cooperating with these memory cells. We have shown elsewhere that virtually all of the Ig-1b memory is carried by Ig-1b bearing cells. In contrast with the lack of suppressor T-cell effect on initial Ig-1b memory cell development, our data show that continued Ig-1b memory development is selectively impaired in suppressed mice. When primed mice are boosted repeatedly with the priming antigen, the average avidity of most of the IgG memory cells increases over 100-fold while there is no avidity increase in the Ig-1b component. To explain these data, we suggest that the development of high avidity memory occurs in two stages. The first stage, which occurs as a result of primary antigenic exposure, is the creation of a pool of IgG-bearing memory cells with a relatively low average avidity for the antigen. The appearance of these first stage memory cells does not require help from (post-thymic) Th, although Th are required for the expression of these memory cells (antibody production). The second stage of B-memory development requires both further antigenic stimulation and B-memory cell interaction with competent Th. This is a continuing process in which the number of memory cells in the pool remains relatively constant but the average avidity of these cells increases with continued antigenic exposure.", "contents": "Two stages of B-cell memory development with different T-cell requirements. We present evidence here for two stages in B-memory cell development, the first of which is T independent and the second T dependent. For these studies, we use a new type of T-deficient mouse (allotype suppressed) which specifically lacks T-helper activity (Th) for a subset of memory B cells responsible for approximately 10% of the overall IgG antibody response. We have shown elsewhere that these mice (SJL X BALB/c hybrids suppressed for Ig-1b) lack Th capable of helping Ig-1b memory cells, although they have normal Th activity for all other IgG memory B cells. This selective Th deficiency allows study of the effects of T depletion on memory development and avidity maturation of one population of B cells under conditions where the bulk of the immune response in the animal is proceeding normally, thus obviating environmental problems due to secondary effects of T depletion. With this sytem, we show that after a single priming dose of 2,4-dinitrophenyl-keyhole limpet hemocyanin, the memory B-cell pool in suppressed and nonsuppressed donors is indistinguishable with respect to magnitude and avidity of the response for all IgG antibodies produced, including Ig-1b antibody, despite the fact that expression of Ig-1b memory cells is prevented in intact Ig-1b-suppressed mice by the absence of Th capbale of cooperating with these memory cells. We have shown elsewhere that virtually all of the Ig-1b memory is carried by Ig-1b bearing cells. In contrast with the lack of suppressor T-cell effect on initial Ig-1b memory cell development, our data show that continued Ig-1b memory development is selectively impaired in suppressed mice. When primed mice are boosted repeatedly with the priming antigen, the average avidity of most of the IgG memory cells increases over 100-fold while there is no avidity increase in the Ig-1b component. To explain these data, we suggest that the development of high avidity memory occurs in two stages. The first stage, which occurs as a result of primary antigenic exposure, is the creation of a pool of IgG-bearing memory cells with a relatively low average avidity for the antigen. The appearance of these first stage memory cells does not require help from (post-thymic) Th, although Th are required for the expression of these memory cells (antibody production). The second stage of B-memory development requires both further antigenic stimulation and B-memory cell interaction with competent Th. This is a continuing process in which the number of memory cells in the pool remains relatively constant but the average avidity of these cells increases with continued antigenic exposure."}
{"id": "PMID:1085326", "title": "Murine B-cell subpopulations responsive to T-dependent and T-independent antigens.", "content": "Strain A/J mice made secondary indirect plaque-forming cell (PFC) responses to azobenzenearsonate (ABA) conjugates of giant keyhole limpet hemocyanin (KLH), a thymic-dependent antigen, but not to conjugates of Ficoll, a T-independent antigen. ABA-Ficoll was also unable to elicit a response in animals primed with ABA-KLH, which have an expanded anti-ABA memory cell pool. On the other hand, ABA-Ficoll rendered mice unresponsive to ABA-KLH when administered before priming or boosting with the T-dependent immunogen. Hence, the T-independent antigen was able to tolerize but unable to trigger B-memory cells responsive to the T-dependent antigen. A/J mice immunized with dinitrophenyl conjugates of Ficoll or bovine IgG (BGG) made vigorous IgM and IgG PFC responses. PFC responses to ABA-KLH and 2,4-dinitrophenyl (DNP)-BGG were abrogated by depleting mice of C3 with cobra venom factor, whereas the IgM and IgG PFC responses to DNP-Ficoll were unaffected. B lymphocytes were fractionated on the basis of receptors for C3 and the subpopulations were assayed for in vitro PFC responses to DNP-Ficoll. Very little response was obtained from complement receptor lymphocyte [CRL(+)] B cells, whereas CRL(-) cells were more responsive than unfractionated B cells. Both populations responded to a polyclonal B-cell mitogen (lipopolysaccharide). On the other hand, the in vitro PFC response to a T-dependent antigen (sheep erythrocytes) correlated with the presence of CRL(+) B cells in the cultures. However, a minor component of this response, sensitive to anti-Thy-1 serum, was made by CRL(-) B cells, indicating the existence of subpopulations of T-dependent B cells with different signalling requirements. The results suggest that most B cells responsive to T-dependent antigens possess receptors for C3 and that C3 plays an obligatory role in the response of these cells. A distinct subpopulation of B cells which lack C3 receptors respond to T-independent antigens. The precursors of PFC for the ABA epitope reside largely or exclusively in the CRL(+) compartment in A/J mice, whereas precursors for the DNP determinant are found in both compartments.", "contents": "Murine B-cell subpopulations responsive to T-dependent and T-independent antigens. Strain A/J mice made secondary indirect plaque-forming cell (PFC) responses to azobenzenearsonate (ABA) conjugates of giant keyhole limpet hemocyanin (KLH), a thymic-dependent antigen, but not to conjugates of Ficoll, a T-independent antigen. ABA-Ficoll was also unable to elicit a response in animals primed with ABA-KLH, which have an expanded anti-ABA memory cell pool. On the other hand, ABA-Ficoll rendered mice unresponsive to ABA-KLH when administered before priming or boosting with the T-dependent immunogen. Hence, the T-independent antigen was able to tolerize but unable to trigger B-memory cells responsive to the T-dependent antigen. A/J mice immunized with dinitrophenyl conjugates of Ficoll or bovine IgG (BGG) made vigorous IgM and IgG PFC responses. PFC responses to ABA-KLH and 2,4-dinitrophenyl (DNP)-BGG were abrogated by depleting mice of C3 with cobra venom factor, whereas the IgM and IgG PFC responses to DNP-Ficoll were unaffected. B lymphocytes were fractionated on the basis of receptors for C3 and the subpopulations were assayed for in vitro PFC responses to DNP-Ficoll. Very little response was obtained from complement receptor lymphocyte [CRL(+)] B cells, whereas CRL(-) cells were more responsive than unfractionated B cells. Both populations responded to a polyclonal B-cell mitogen (lipopolysaccharide). On the other hand, the in vitro PFC response to a T-dependent antigen (sheep erythrocytes) correlated with the presence of CRL(+) B cells in the cultures. However, a minor component of this response, sensitive to anti-Thy-1 serum, was made by CRL(-) B cells, indicating the existence of subpopulations of T-dependent B cells with different signalling requirements. The results suggest that most B cells responsive to T-dependent antigens possess receptors for C3 and that C3 plays an obligatory role in the response of these cells. A distinct subpopulation of B cells which lack C3 receptors respond to T-independent antigens. The precursors of PFC for the ABA epitope reside largely or exclusively in the CRL(+) compartment in A/J mice, whereas precursors for the DNP determinant are found in both compartments."}
{"id": "PMID:1085327", "title": "Heterogeneity of murine regulatory T cells. I. Subpopulations of amplifier and suppressor T cells.", "content": "Immunization of C3H/HeJ mice with 4 X 10(9) SRBC yields a whole splenic T-cell population which can, upon transfer, specifically suppress recipient direct and indirect plaque-forming cells (PFC) responses to sheep erythrocytes (SRBC). Discontinuous bovine serum albumin density gradient fractionation of these T cells demonstrated a population of low density T cells which augmented and a population of high density T cells which suppressed recipient responses irrespective of the number of T cells transferred. Moreover, infusion of admixtures of low and high density cells resulted in intermediate regulatory functions which could be predicted by knowing the regulatory capacity of each population alone. In addition to heterogeneity existing among regulatory T cells as regards amplification and suppression, it appeared that heterogeneity existed within the suppressor T population. Thus, T cells capable of inhibiting direct PFC could be distinguished from those suppressing indirect PFC by their differential localization in peripheral lymphoid tissue, differences in the dissipation of suppressive influences during incubation at 37 degrees C, and by differences in the possible requirement for adherent cell populations. While the relative frequency of both low density amplifier and high density suppressor cells increased with the dose of SRBC used for their induction, it appeared that suppressor cells might be generated in response to feedback signals from amplifier cells. These studies indicate that further delineation of heterogeneity existing within suppressor populations may be helpful in defining mechanisms required for the induction and manifestation of suppressive regulatory forces.", "contents": "Heterogeneity of murine regulatory T cells. I. Subpopulations of amplifier and suppressor T cells. Immunization of C3H/HeJ mice with 4 X 10(9) SRBC yields a whole splenic T-cell population which can, upon transfer, specifically suppress recipient direct and indirect plaque-forming cells (PFC) responses to sheep erythrocytes (SRBC). Discontinuous bovine serum albumin density gradient fractionation of these T cells demonstrated a population of low density T cells which augmented and a population of high density T cells which suppressed recipient responses irrespective of the number of T cells transferred. Moreover, infusion of admixtures of low and high density cells resulted in intermediate regulatory functions which could be predicted by knowing the regulatory capacity of each population alone. In addition to heterogeneity existing among regulatory T cells as regards amplification and suppression, it appeared that heterogeneity existed within the suppressor T population. Thus, T cells capable of inhibiting direct PFC could be distinguished from those suppressing indirect PFC by their differential localization in peripheral lymphoid tissue, differences in the dissipation of suppressive influences during incubation at 37 degrees C, and by differences in the possible requirement for adherent cell populations. While the relative frequency of both low density amplifier and high density suppressor cells increased with the dose of SRBC used for their induction, it appeared that suppressor cells might be generated in response to feedback signals from amplifier cells. These studies indicate that further delineation of heterogeneity existing within suppressor populations may be helpful in defining mechanisms required for the induction and manifestation of suppressive regulatory forces."}
{"id": "PMID:1085328", "title": "Expression of Ia antigens on hapten-specific B cells. I. Delineation of B-cell subpopulations.", "content": "The nonimmune adult spleen contains at least two B-cell subpopulations. The majority of primary B cells express cell surface Ia determinants and have the capacity to give rise to IgG antibody-producing clones after T-cell dependent antigenic stimulation. There is also a small subpopulation of primary B cells which are, by definition, Ia negative, since their activity is not eliminated by negative selection with anti-Ia serum and complement. The Ia-negative B cells give rise to clones that produce only IgM antibody. These B-cell subsets may form a continuum in B-cell maturation, or they may exist as discrete B-cell lineages. Since the cellular expression of Ia antigens appears to correlate with the ability of the B cell to generate IgG-producing clones, it is speculated that Ia molecules may have a role in the IgM to IgG B-cell switch mechanism.", "contents": "Expression of Ia antigens on hapten-specific B cells. I. Delineation of B-cell subpopulations. The nonimmune adult spleen contains at least two B-cell subpopulations. The majority of primary B cells express cell surface Ia determinants and have the capacity to give rise to IgG antibody-producing clones after T-cell dependent antigenic stimulation. There is also a small subpopulation of primary B cells which are, by definition, Ia negative, since their activity is not eliminated by negative selection with anti-Ia serum and complement. The Ia-negative B cells give rise to clones that produce only IgM antibody. These B-cell subsets may form a continuum in B-cell maturation, or they may exist as discrete B-cell lineages. Since the cellular expression of Ia antigens appears to correlate with the ability of the B cell to generate IgG-producing clones, it is speculated that Ia molecules may have a role in the IgM to IgG B-cell switch mechanism."}
{"id": "PMID:1085329", "title": "T-Lymphocyte precursors. I. Synergy between precursor and mature T lymphocytes in the response to concanavalin A.", "content": "When mouse bone marrow cells are mixed with cortisol-resistant thymocytes and stimulated in vitro with concanavalin A, the mitogenic response observed is much greater than additive, that is, it is synergistic. Between 94 and 96% of responding cells could be identified as T cells (Thy-1 positive) and of these, 79-100% derived from the cortisol-resistant thymocyte population, not from the bone marrow. Purified macrophages could not replace bone marrow; and marrow depleted of mature T or B cells worked as well as normal marrow. Thus, T and B cells and macrophages were ruled out as the synergizing cell of bone marrow. Nude spleen contained 10 times as many precursors of T cells as did nude marrow and was 10 times better at synergy with cortisol-resistant thymocytes. This implication of the pre-T cell as synergizer was supported by the finding that the synergistic activity of marrow was lost on preincubation, but maintained if the preincubation medium contained thymosin or cyclic AMP. Thus, the ability to enhance the response of relatively mature T cells to Con A is a property of pre-T cells. It is anticipated that this property will allow more detailed studies of T-cell precursor development in mice, and possibly in man.", "contents": "T-Lymphocyte precursors. I. Synergy between precursor and mature T lymphocytes in the response to concanavalin A. When mouse bone marrow cells are mixed with cortisol-resistant thymocytes and stimulated in vitro with concanavalin A, the mitogenic response observed is much greater than additive, that is, it is synergistic. Between 94 and 96% of responding cells could be identified as T cells (Thy-1 positive) and of these, 79-100% derived from the cortisol-resistant thymocyte population, not from the bone marrow. Purified macrophages could not replace bone marrow; and marrow depleted of mature T or B cells worked as well as normal marrow. Thus, T and B cells and macrophages were ruled out as the synergizing cell of bone marrow. Nude spleen contained 10 times as many precursors of T cells as did nude marrow and was 10 times better at synergy with cortisol-resistant thymocytes. This implication of the pre-T cell as synergizer was supported by the finding that the synergistic activity of marrow was lost on preincubation, but maintained if the preincubation medium contained thymosin or cyclic AMP. Thus, the ability to enhance the response of relatively mature T cells to Con A is a property of pre-T cells. It is anticipated that this property will allow more detailed studies of T-cell precursor development in mice, and possibly in man."}
{"id": "PMID:1085330", "title": "T-cell-deficient mice produce more antihapten antibodies against syngeneic than against allogeneic erythrocyte conjugates.", "content": "T-cell-deficient mice, either anti-thymocyte serum treated or nude mice, were immunized with hapten (4-hydroxy-3,5-dinitrophenyl acetic acid, NNP) conjugates of syngeneic, allogeneic, or xenogeneic erythrocytes. Immunization with syngeneic conjugates led to a stronger anti-NNP response than immunization with allogeneic or xenogeneic conjugates. A study of congenic mouse strains suggested that a prerequisite for this effect was that immunogenic erythrocytes and responding animals shared H-2-controlled characteristics. F1 hybrid erythrocyte conjugates injected into F1 hybrid mice behaved like other syngeneic erythrocytes. The same erythrocyte conjugates injected into either parental strain induced a weak response indistinguishable from the response to allogeneic erythrocyte conjugates. Parental erythrocyte conjugates injected into F1 mice induced an anti-NNP response that was significantly lower than the response to F1 erythrocyte conjugates but significantly higher than the response to allogeneic conjugates. The response of normal mice to syngeneic erythrocytes was weaker than the response of T-cell-deficient mice, which could have been caused by suppressor T cells. Their response to allogeneic conjugates was higher than the response of T-cell-deficient mice and the response to xenogeneic conjugates higher still. This was probably due to allo- or xenoreactive helper cells.", "contents": "T-cell-deficient mice produce more antihapten antibodies against syngeneic than against allogeneic erythrocyte conjugates. T-cell-deficient mice, either anti-thymocyte serum treated or nude mice, were immunized with hapten (4-hydroxy-3,5-dinitrophenyl acetic acid, NNP) conjugates of syngeneic, allogeneic, or xenogeneic erythrocytes. Immunization with syngeneic conjugates led to a stronger anti-NNP response than immunization with allogeneic or xenogeneic conjugates. A study of congenic mouse strains suggested that a prerequisite for this effect was that immunogenic erythrocytes and responding animals shared H-2-controlled characteristics. F1 hybrid erythrocyte conjugates injected into F1 hybrid mice behaved like other syngeneic erythrocytes. The same erythrocyte conjugates injected into either parental strain induced a weak response indistinguishable from the response to allogeneic erythrocyte conjugates. Parental erythrocyte conjugates injected into F1 mice induced an anti-NNP response that was significantly lower than the response to F1 erythrocyte conjugates but significantly higher than the response to allogeneic conjugates. The response of normal mice to syngeneic erythrocytes was weaker than the response of T-cell-deficient mice, which could have been caused by suppressor T cells. Their response to allogeneic conjugates was higher than the response of T-cell-deficient mice and the response to xenogeneic conjugates higher still. This was probably due to allo- or xenoreactive helper cells."}
{"id": "PMID:1085331", "title": "H-2 compatibility requirement for virus-specific T-cell-mediated cytolysis. Evaluation of the role of H-2I region and non-H-2 genes in regulating immune response.", "content": "Lymphocytic choriomeningitis virus (LCMV) and ectromelia virus-specific T-cell-mediated cytotoxicity was assayed in various strain combinations using as targets peritoneal macrophages which have been shown to express Ia antigens. Virus-specific cytotoxicity was found only in H-2K- or D-region compatible combinations. I-region compatibility was not necessary nor alone sufficient for lysis. Six different I-region specificities had no obvious effect on the capacity to generate in vivo specific cytotoxicity (expressed in vitro) associated with Dd. Low LCMV-specific cytotoxic activity generated in DBA/2 mice was caused by the non-H-2 genetic background. This trait was inversely related to the infectious virus dose and recessive. Non-H-2 genes, possibly involved in controlling initial spread and multiplication of virus, seem to be, at least in the examples tested, more important in determining virus-specific cytotoxic T-cell activity in spleens than are Ir genes coded in H-2.", "contents": "H-2 compatibility requirement for virus-specific T-cell-mediated cytolysis. Evaluation of the role of H-2I region and non-H-2 genes in regulating immune response. Lymphocytic choriomeningitis virus (LCMV) and ectromelia virus-specific T-cell-mediated cytotoxicity was assayed in various strain combinations using as targets peritoneal macrophages which have been shown to express Ia antigens. Virus-specific cytotoxicity was found only in H-2K- or D-region compatible combinations. I-region compatibility was not necessary nor alone sufficient for lysis. Six different I-region specificities had no obvious effect on the capacity to generate in vivo specific cytotoxicity (expressed in vitro) associated with Dd. Low LCMV-specific cytotoxic activity generated in DBA/2 mice was caused by the non-H-2 genetic background. This trait was inversely related to the infectious virus dose and recessive. Non-H-2 genes, possibly involved in controlling initial spread and multiplication of virus, seem to be, at least in the examples tested, more important in determining virus-specific cytotoxic T-cell activity in spleens than are Ir genes coded in H-2."}
{"id": "PMID:1085332", "title": "Terminal deoxynucleotidyl transferase is found in prothymocytes.", "content": "Terminal deoxynucleotidyl transferase is an enzyme which has the unique property of polymerizing polydeoxynucleotides onto a primer in the absence of a template (1,2). This enzyme is found both in the thymus and the bone marrow of birds, rodents, and humans (3-7). Whether the marrow cells that contain terminal transferase are related to thymocytes, or are on a separate pathway of differentiation, is not yet known (7,8). To determine the lineage of the murine bone marrow cells that have terminal transferase, we have investigated whether these cells have the antigen Thy-1 induced on the cells by treatment with thymopoietin (9). Thymopoietin is known to induce a set of characteristic T-cell markers including the Thy-1 alloantigen on the surface of a subpopulation of bone marrow cells committed to T-cell differentiation (prothymocytes) (10). Destruction of Thy- 1-positive cells after exposure to thymopoietin allows elimination of a substantial fraction of those bone marrow cells that can repopulate an irradiated thymus (11). We find that such an elimination after induction with the thymic polypeptide removes a substantial amount of terminal transferase from the bone marrow cell population, suggesting that at least one-half of the marrow cells bearing this enzyme are related to those found in the thymus.", "contents": "Terminal deoxynucleotidyl transferase is found in prothymocytes. Terminal deoxynucleotidyl transferase is an enzyme which has the unique property of polymerizing polydeoxynucleotides onto a primer in the absence of a template (1,2). This enzyme is found both in the thymus and the bone marrow of birds, rodents, and humans (3-7). Whether the marrow cells that contain terminal transferase are related to thymocytes, or are on a separate pathway of differentiation, is not yet known (7,8). To determine the lineage of the murine bone marrow cells that have terminal transferase, we have investigated whether these cells have the antigen Thy-1 induced on the cells by treatment with thymopoietin (9). Thymopoietin is known to induce a set of characteristic T-cell markers including the Thy-1 alloantigen on the surface of a subpopulation of bone marrow cells committed to T-cell differentiation (prothymocytes) (10). Destruction of Thy- 1-positive cells after exposure to thymopoietin allows elimination of a substantial fraction of those bone marrow cells that can repopulate an irradiated thymus (11). We find that such an elimination after induction with the thymic polypeptide removes a substantial amount of terminal transferase from the bone marrow cell population, suggesting that at least one-half of the marrow cells bearing this enzyme are related to those found in the thymus."}
{"id": "PMID:1085333", "title": "Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes.", "content": "A system was established to assess the requirement for continuous presence of antigen in B-lymphocyte activation to antibody formation. Mouse spleen B lymphocytes, enriched for cells bearing anti-NIP (hapten 4-hydroxy-3-iodo-5-nitrophenylacetic acid) receptors, were pretreated briefly with NIP-POL (polymerized flagellin) antigen, washed, and added in small numbers to microcultures. The behaviour of these cells was compared with that of cells cultured in the continuous presence of antigen. Unfractionated spleen cells were studied under similar conditions. In contrast to unfractionated cells, enriched cells could not be triggered effectively by brief contact with antigen at any concentration tested. Fewer cells were activated, and clone size was smaller after brief contact with antigen than when antigen was present continuously. Furthermore, brief contact at high concentration did not cause tolerance induction.", "contents": "Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes. A system was established to assess the requirement for continuous presence of antigen in B-lymphocyte activation to antibody formation. Mouse spleen B lymphocytes, enriched for cells bearing anti-NIP (hapten 4-hydroxy-3-iodo-5-nitrophenylacetic acid) receptors, were pretreated briefly with NIP-POL (polymerized flagellin) antigen, washed, and added in small numbers to microcultures. The behaviour of these cells was compared with that of cells cultured in the continuous presence of antigen. Unfractionated spleen cells were studied under similar conditions. In contrast to unfractionated cells, enriched cells could not be triggered effectively by brief contact with antigen at any concentration tested. Fewer cells were activated, and clone size was smaller after brief contact with antigen than when antigen was present continuously. Furthermore, brief contact at high concentration did not cause tolerance induction."}
{"id": "PMID:1085334", "title": "Induction of an antibody response in cultures of human peripheral blood lymphocytes.", "content": "A culture system is descirbed which provides adequate conditions for in vitro immunization of humand peripheral blood lymphocytes to heterologous erythrocytes. Making use of this method we could obtain, with a number of different donors, an antibody response which peaked at about day 8 of culture with 30-300 plaque-forming cells (PFC) per 10(6) input lymphocytes. However, in a number of experiments poor or negative results were obtained, even with donors that had previously given good response. This variability in the results was shown not to be due to a too low number of precursor cells present in the blood and could be overcome by treating the cells, before initiation of the culture, with a factor produced by mouse T cells educated to sheep erythrocytes (SRBC). Under these conditions a PFC responce was obtained which peaked at about day 8 and which in some experiments could be as high as 20,000 PFC per 10(6) input lymphocytes. Paralleling the increase in PFC was an increase in cell number. The cells recovered from the treated cultures were at all times more numerous than in the nontreated cultures. The height of both the proliferative and antibody-producing responses varied from experiment to experiment, a higher proliferative response, accompanying a higher PFC response. Although the mechanisms that are at the basis of the antibody response in vitro described in this paper still need to be clarified, this system may become a useful tool in studying the immune response in man.", "contents": "Induction of an antibody response in cultures of human peripheral blood lymphocytes. A culture system is descirbed which provides adequate conditions for in vitro immunization of humand peripheral blood lymphocytes to heterologous erythrocytes. Making use of this method we could obtain, with a number of different donors, an antibody response which peaked at about day 8 of culture with 30-300 plaque-forming cells (PFC) per 10(6) input lymphocytes. However, in a number of experiments poor or negative results were obtained, even with donors that had previously given good response. This variability in the results was shown not to be due to a too low number of precursor cells present in the blood and could be overcome by treating the cells, before initiation of the culture, with a factor produced by mouse T cells educated to sheep erythrocytes (SRBC). Under these conditions a PFC responce was obtained which peaked at about day 8 and which in some experiments could be as high as 20,000 PFC per 10(6) input lymphocytes. Paralleling the increase in PFC was an increase in cell number. The cells recovered from the treated cultures were at all times more numerous than in the nontreated cultures. The height of both the proliferative and antibody-producing responses varied from experiment to experiment, a higher proliferative response, accompanying a higher PFC response. Although the mechanisms that are at the basis of the antibody response in vitro described in this paper still need to be clarified, this system may become a useful tool in studying the immune response in man."}
{"id": "PMID:1085335", "title": "Helper function of T cells depleted of alloantigen-reactive lymphocytes by filtration through irradiated F1 hybrid recipients. I. Failure to collaborate with allogeneic B cells in a secondary response to sheep erythrocytes measured in vivo.", "content": "Helper T cells were obtained by injecting heavily irradiated semiallogeneic mice with lymph node cells from H-2-incompatible parental strain mice primed with sheep erythrocytes (SRC) 2 mo before. Thoracic duct lymphocytes collected from the recipents 18-40 h later (nearly all of which were theta-positive and of donor origin) were totally and specifically unresponsive against host-type determinants in mixed-lymphocyte culture. The filtered cells were transferred to irradiated semiallogeneic mice together with SRC and anti-theta-serum-treated (B) cells from SRC-primed syngeneic, semiallogeneic, or allogeneic mice. When antibody-forming cells were measured in the spleen 5-9 days later, effective IgM and IgG collaborative responses were observed with both syngeneic and semiallogeneic B cells but not with allogeneic B cells. No evidence was found that the failure to obtain collaboration with the allogeneic B cells was due to inhibition of the B cells by the T cells or vice versa.", "contents": "Helper function of T cells depleted of alloantigen-reactive lymphocytes by filtration through irradiated F1 hybrid recipients. I. Failure to collaborate with allogeneic B cells in a secondary response to sheep erythrocytes measured in vivo. Helper T cells were obtained by injecting heavily irradiated semiallogeneic mice with lymph node cells from H-2-incompatible parental strain mice primed with sheep erythrocytes (SRC) 2 mo before. Thoracic duct lymphocytes collected from the recipents 18-40 h later (nearly all of which were theta-positive and of donor origin) were totally and specifically unresponsive against host-type determinants in mixed-lymphocyte culture. The filtered cells were transferred to irradiated semiallogeneic mice together with SRC and anti-theta-serum-treated (B) cells from SRC-primed syngeneic, semiallogeneic, or allogeneic mice. When antibody-forming cells were measured in the spleen 5-9 days later, effective IgM and IgG collaborative responses were observed with both syngeneic and semiallogeneic B cells but not with allogeneic B cells. No evidence was found that the failure to obtain collaboration with the allogeneic B cells was due to inhibition of the B cells by the T cells or vice versa."}
{"id": "PMID:1085336", "title": "Loss of suppressor T cells in adult NZB/NZW mice.", "content": "We have investigated suppressor T-cell activity in female NZB/NZW F1 mice using PWM-driven IgM biosynthesis in vitro as an indicator system. In initial we studied we observed that spleen cells from normal mice (BALB/c, C57BL/6), as well as from young (4 wk) and adult (18 wk) NZB/NZW mice, cultured in the presence of PWM synthesize 860 +/- 120 ng IgM/10(6) cells/7 days. However, when Con A (at 2 mug/ml) was added directly to the cultures (along with PWM), cells obtained from adult normal mice and young NZB/NZW mice showed a 94% suppression of IgM synthesis, whereas cells obtained from adult NZB/NZW mice were suppressed significantly less. To analyze these findings we studied the effect of Con A-induced suppressor cells (cells cultured with Con A for 24 h and washed free of Con A) on PWM-driven IgM biosynthesis. Spleen cells obtained from normal mice cultured in the presence of Con A-pulsed cells obtained from normal mice and young NZB/NZW mice showed an 83-88% suppression of PWM-driven IgM synthesis. Similarly, supernates obtained from Con A-pulsed cells of normal mice or of young NZB/NZW mice suppressed PWM-driven IgM synthesis. This suppression by Con A-pulsed cells and their supernates required T cells since T-cell fractions but not B-cell fractions eluted from anti-Fab Sephadex columns mediated suppression of co-cultured normal cells; in addition, Con A-pulsed cells treated with anti-theta and complement do not mediate suppression. These studies of Con A-induced suppressor cell activity in normal mice and young NZB/NZW mice contrast with studies of Con A-induced suppressor cell activity in adult NZB/NZW mice. We found that adult NZB/NZW Con A-pulsed cells and supernates obtained from the Con A-pulse cells had vastly decreased suppressor potential; in this case the Con A-pulse cells and supernatant fluids derived from such cells did not suppress PWM-driven IgM synthesis by normal cells. Finally, whereas spleen cells from young and adult NZB/NZW mice differ in their suppressor cell potential, cells from both sources could respond equally to suppressor signals in that Con A-pulsed normal cells or supernates derived from such cells caused equivalent suppression of PWM-driven IgM synthesis by young and adult NZB/NZW cells. These observations allow us to conclude that NZB/NZW mice lose suppressor T-cell activity as they age.", "contents": "Loss of suppressor T cells in adult NZB/NZW mice. We have investigated suppressor T-cell activity in female NZB/NZW F1 mice using PWM-driven IgM biosynthesis in vitro as an indicator system. In initial we studied we observed that spleen cells from normal mice (BALB/c, C57BL/6), as well as from young (4 wk) and adult (18 wk) NZB/NZW mice, cultured in the presence of PWM synthesize 860 +/- 120 ng IgM/10(6) cells/7 days. However, when Con A (at 2 mug/ml) was added directly to the cultures (along with PWM), cells obtained from adult normal mice and young NZB/NZW mice showed a 94% suppression of IgM synthesis, whereas cells obtained from adult NZB/NZW mice were suppressed significantly less. To analyze these findings we studied the effect of Con A-induced suppressor cells (cells cultured with Con A for 24 h and washed free of Con A) on PWM-driven IgM biosynthesis. Spleen cells obtained from normal mice cultured in the presence of Con A-pulsed cells obtained from normal mice and young NZB/NZW mice showed an 83-88% suppression of PWM-driven IgM synthesis. Similarly, supernates obtained from Con A-pulsed cells of normal mice or of young NZB/NZW mice suppressed PWM-driven IgM synthesis. This suppression by Con A-pulsed cells and their supernates required T cells since T-cell fractions but not B-cell fractions eluted from anti-Fab Sephadex columns mediated suppression of co-cultured normal cells; in addition, Con A-pulsed cells treated with anti-theta and complement do not mediate suppression. These studies of Con A-induced suppressor cell activity in normal mice and young NZB/NZW mice contrast with studies of Con A-induced suppressor cell activity in adult NZB/NZW mice. We found that adult NZB/NZW Con A-pulsed cells and supernates obtained from the Con A-pulse cells had vastly decreased suppressor potential; in this case the Con A-pulse cells and supernatant fluids derived from such cells did not suppress PWM-driven IgM synthesis by normal cells. Finally, whereas spleen cells from young and adult NZB/NZW mice differ in their suppressor cell potential, cells from both sources could respond equally to suppressor signals in that Con A-pulsed normal cells or supernates derived from such cells caused equivalent suppression of PWM-driven IgM synthesis by young and adult NZB/NZW cells. These observations allow us to conclude that NZB/NZW mice lose suppressor T-cell activity as they age."}
{"id": "PMID:1085337", "title": "Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes.", "content": "Data presented here show that locidentify in the I-region of the H-2 gene complex are selectively expressed in different functional T-cell subpopulations. These loci are closely linked (or possibly identical) to loci that control immune responses. They control surface determinants which identify helper and suppressor T lymphocytes. Determinants described here on allotype suppressor T cells (Ts) are found on normal (nonsuppressed) lymphoid cells, but are not found on helper T cells (Th). These determinants are controlled by a locus mapping in the I region of the H-2 complex. In an accompanying publication we show that this locus (Ia-4) marks a new I subregion (I-J) and is expressed only on T cells. Thus Ia-4 determinants idenfity a T-cell subpopulation which includes Ts but not Th. Th also carry identifying surface determinants controlled by loci that map to the H-2 complex, probably within the I region. These determinants are not found on Ts. Data presented also establish that loci in the I region control determinants on Th, but do not conclusively demonstrate that these are the determinants that distinguish Th from Ts. The selective expression of H-2-controlled  determinants on Ts and Th suggests that these determinants are directly involved in immunoregulation.", "contents": "Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes. Data presented here show that locidentify in the I-region of the H-2 gene complex are selectively expressed in different functional T-cell subpopulations. These loci are closely linked (or possibly identical) to loci that control immune responses. They control surface determinants which identify helper and suppressor T lymphocytes. Determinants described here on allotype suppressor T cells (Ts) are found on normal (nonsuppressed) lymphoid cells, but are not found on helper T cells (Th). These determinants are controlled by a locus mapping in the I region of the H-2 complex. In an accompanying publication we show that this locus (Ia-4) marks a new I subregion (I-J) and is expressed only on T cells. Thus Ia-4 determinants idenfity a T-cell subpopulation which includes Ts but not Th. Th also carry identifying surface determinants controlled by loci that map to the H-2 complex, probably within the I region. These determinants are not found on Ts. Data presented also establish that loci in the I region control determinants on Th, but do not conclusively demonstrate that these are the determinants that distinguish Th from Ts. The selective expression of H-2-controlled  determinants on Ts and Th suggests that these determinants are directly involved in immunoregulation."}
{"id": "PMID:1085338", "title": "A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes.", "content": "In an accompanying publication we show that a subpopulation of T lymphocytes, which includes allotype suppressor T cells, selectively expresses I-region determinants. In this report, we show that these determinants are controlled by a new locus, Ia-4. Unlike the classically defined Ia antigens, they are not found on B lymphocytes. Antibody against Ia-4 determinants cannot be detected by conventional dye exclusion cytoxicity assays, suggesting that they are present on a small subpopulation (less than 10%) of peripheral T lymphocytes. The Ia-4 locus marks a new I subregion, provisionally designated I-J. This chromosomal segment is defined by the crossover positions in strains B10.A(5R) (K-end boundary) and B10.HTT (D-end boundary), and maps between the I-B and I-C subregions.", "contents": "A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. In an accompanying publication we show that a subpopulation of T lymphocytes, which includes allotype suppressor T cells, selectively expresses I-region determinants. In this report, we show that these determinants are controlled by a new locus, Ia-4. Unlike the classically defined Ia antigens, they are not found on B lymphocytes. Antibody against Ia-4 determinants cannot be detected by conventional dye exclusion cytoxicity assays, suggesting that they are present on a small subpopulation (less than 10%) of peripheral T lymphocytes. The Ia-4 locus marks a new I subregion, provisionally designated I-J. This chromosomal segment is defined by the crossover positions in strains B10.A(5R) (K-end boundary) and B10.HTT (D-end boundary), and maps between the I-B and I-C subregions."}
{"id": "PMID:1085339", "title": "Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex.", "content": "The locus of the gene that codes for the antigen-specific suppressive T-cell factor was determined to be in a new subregion \"I-J\" which locates between I-B and I-C subregions in the H-2 histocompatibility complex. This was shown by two different lines of evidence: (a) The absorbing capacity for the suppressive T-cell factor of several alloantisera against restricted I subregions did not correlate with their specificity for previously known Ia molecules which are coded for by genes in I-A and I-C subregions, but was associated with the specificity for the products of genes putatively present between I-B and I-C subregions. By the occurrence of special recombinant strains, i.e. B10.A(5R), B10.A(3R), B10.S(9R), and B10.HTT, which differ with respect to the I-J subregion, we were able to produce alloantisera which distinguish I-J subregion gene products. The absorption studies using these special alloantisera directed to I-J subregion clearly indicated that the suppressive T-cell factor is a product of I-J subregion gene(s), and that the molecule is distinct from known Ia molecules expressed on splenic B cells. (b) Taking advantage of the fact that there is a strict histocompatibility requirement for the effective suppression between the donor and recipient strains of the suppressive T-cell factor, we were able to determine the required identities of the genes in the H-2 complex existing among those present between I-B and I-C. Again, utilizing the T-cell factors obtained from special recombinant strains, i.e. B10.A(4R) and B10.A(5R), we were able to locate the gene that codes for the suppressive T-cell factor reactive only with relevant haplotype strains between I-B and I-C subregions. These results are most reasonably explained by the presence of a new subregion I-J which is specialized in coding for the suppressive T-cell factor as a different molecule from previously known Ia molecules.", "contents": "Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. The locus of the gene that codes for the antigen-specific suppressive T-cell factor was determined to be in a new subregion \"I-J\" which locates between I-B and I-C subregions in the H-2 histocompatibility complex. This was shown by two different lines of evidence: (a) The absorbing capacity for the suppressive T-cell factor of several alloantisera against restricted I subregions did not correlate with their specificity for previously known Ia molecules which are coded for by genes in I-A and I-C subregions, but was associated with the specificity for the products of genes putatively present between I-B and I-C subregions. By the occurrence of special recombinant strains, i.e. B10.A(5R), B10.A(3R), B10.S(9R), and B10.HTT, which differ with respect to the I-J subregion, we were able to produce alloantisera which distinguish I-J subregion gene products. The absorption studies using these special alloantisera directed to I-J subregion clearly indicated that the suppressive T-cell factor is a product of I-J subregion gene(s), and that the molecule is distinct from known Ia molecules expressed on splenic B cells. (b) Taking advantage of the fact that there is a strict histocompatibility requirement for the effective suppression between the donor and recipient strains of the suppressive T-cell factor, we were able to determine the required identities of the genes in the H-2 complex existing among those present between I-B and I-C. Again, utilizing the T-cell factors obtained from special recombinant strains, i.e. B10.A(4R) and B10.A(5R), we were able to locate the gene that codes for the suppressive T-cell factor reactive only with relevant haplotype strains between I-B and I-C subregions. These results are most reasonably explained by the presence of a new subregion I-J which is specialized in coding for the suppressive T-cell factor as a different molecule from previously known Ia molecules."}
{"id": "PMID:1085340", "title": "H-2 restriction of virus-specific T-cell-mediated effector functions in vivo. II. Adoptive transfer of delayed-type hypersensitivity to murine lymphocytic choriomeningits virus is restriced by the K and D region of H-2.", "content": "In mice, primary footpad swelling after local infection with lymphocytic choriomeningitis virus (LCMV) and delayed-type hypersensitivity (DTH) adoptively transferred by LCMV immune lymphocytes are T-cell dependent. Nude mice do not develop primary footpad swelling, and T-cell depletion abrogates the capacity to transfer LCMV-specific DTH. Effector T cells involved in eliciting dose-dependent DTH are virus specific in that vaccinia virus-immune lymphocytes could not elicit DTH in LCMV-infected mice. The adoptive transfer of DTH is restricted to H-2K or H-2D compatible donor-recipient combinations. Distinct from the fowl-gamma-globulin DTH model, I-region compatibility is neither necessary nor alone sufficient. Whatever the mechanisms involved in this K- or D-region associated restriction in vivo, it most likely operates at the level of T-cell recognition of \"altered self\" coded in K or D. T cells associated with the I region (helper T cells and DTH-T cells to fowl-gamma-globulin) are specific for soluble, defined, and inert antigens. T cells associated with the K and D region (T cells cytotoxic in vitro and in vivo for acute LCMV-infected cells, DTH effector T cells, and anti-viral T cells) are specific for infectious, multiplying virus. The fact that T-cell specificity is differentially linked with the I region or with the K and D regions of H-2 may reflect the fundamental biological differences of these antigens. Although it cannot be excluded that separate functional subclasses of T-effector cells could have self-recognizers for different cell surface structures coded in I or K and D, it is more likely that the antigen parameters determine whether T cells are specific for \"altered\" I or \"altered\" K- or D-coded structures.", "contents": "H-2 restriction of virus-specific T-cell-mediated effector functions in vivo. II. Adoptive transfer of delayed-type hypersensitivity to murine lymphocytic choriomeningits virus is restriced by the K and D region of H-2. In mice, primary footpad swelling after local infection with lymphocytic choriomeningitis virus (LCMV) and delayed-type hypersensitivity (DTH) adoptively transferred by LCMV immune lymphocytes are T-cell dependent. Nude mice do not develop primary footpad swelling, and T-cell depletion abrogates the capacity to transfer LCMV-specific DTH. Effector T cells involved in eliciting dose-dependent DTH are virus specific in that vaccinia virus-immune lymphocytes could not elicit DTH in LCMV-infected mice. The adoptive transfer of DTH is restricted to H-2K or H-2D compatible donor-recipient combinations. Distinct from the fowl-gamma-globulin DTH model, I-region compatibility is neither necessary nor alone sufficient. Whatever the mechanisms involved in this K- or D-region associated restriction in vivo, it most likely operates at the level of T-cell recognition of \"altered self\" coded in K or D. T cells associated with the I region (helper T cells and DTH-T cells to fowl-gamma-globulin) are specific for soluble, defined, and inert antigens. T cells associated with the K and D region (T cells cytotoxic in vitro and in vivo for acute LCMV-infected cells, DTH effector T cells, and anti-viral T cells) are specific for infectious, multiplying virus. The fact that T-cell specificity is differentially linked with the I region or with the K and D regions of H-2 may reflect the fundamental biological differences of these antigens. Although it cannot be excluded that separate functional subclasses of T-effector cells could have self-recognizers for different cell surface structures coded in I or K and D, it is more likely that the antigen parameters determine whether T cells are specific for \"altered\" I or \"altered\" K- or D-coded structures."}
{"id": "PMID:1085341", "title": "Studies on the mechanism of phagocytosis. II. The interaction of macrophages with anti-immunoglobulin IgG-coated bone marrow-derived lymphocytes.", "content": "We have examined the effect of the distribution of anti-immunoglobulin IgG molecules on the surface of bone marrow-derived lymphocytes upon the interaction of these cells with macrophages. Lymphocytes which were diffusely coated with antibodies to surface immunoglogulin were ingested by macrophages. Lymphocytes which had the same number of anti-immunoglobulin IgG molecules redistributed to one pole of the surface bound to the macrophages' Fc receptors but were not ingested. These results confirm our previous hypothesis that ingestion of an immunologically coated particle requires the sequential, circumferential binding of specific receptors on the plasma membrane of a phagocytic cell to immunologic ligands distributed over the entire particle surface. Macrophages which had bound capped lymphocytes by the macrophages' Fc receptors removed the immune complex caps from the lymphocyte surface without destroying the lymphocytes. These lymphocytes remained attached to the macrophage surface. The finding that macrophages can phagocytize immune complexes from the surface of a cell without destroying the cell to which these complexes are attached may be important in understanding the effects of antigens and antibodies on cells participating in a humoral immune response, in identifying the mechanisms by which chronic viral infections are established, and in defining the roles of blocking antibodies in tumor immunity.", "contents": "Studies on the mechanism of phagocytosis. II. The interaction of macrophages with anti-immunoglobulin IgG-coated bone marrow-derived lymphocytes. We have examined the effect of the distribution of anti-immunoglobulin IgG molecules on the surface of bone marrow-derived lymphocytes upon the interaction of these cells with macrophages. Lymphocytes which were diffusely coated with antibodies to surface immunoglogulin were ingested by macrophages. Lymphocytes which had the same number of anti-immunoglobulin IgG molecules redistributed to one pole of the surface bound to the macrophages' Fc receptors but were not ingested. These results confirm our previous hypothesis that ingestion of an immunologically coated particle requires the sequential, circumferential binding of specific receptors on the plasma membrane of a phagocytic cell to immunologic ligands distributed over the entire particle surface. Macrophages which had bound capped lymphocytes by the macrophages' Fc receptors removed the immune complex caps from the lymphocyte surface without destroying the lymphocytes. These lymphocytes remained attached to the macrophage surface. The finding that macrophages can phagocytize immune complexes from the surface of a cell without destroying the cell to which these complexes are attached may be important in understanding the effects of antigens and antibodies on cells participating in a humoral immune response, in identifying the mechanisms by which chronic viral infections are established, and in defining the roles of blocking antibodies in tumor immunity."}
{"id": "PMID:1085342", "title": "Secretion of Ia antigens by a subpopulation of T cells which are Ly-1+, Ly-2-, and Ia-.", "content": "It was found that Ia antignes are rapidly secreted by a subpopulation of splenic T lymphocytes which are nonadherent and which express the surface phenotype Ly-1+, Ly-2-, and Ia-. Secretion of the Ia antigens was a metabolically active process which was inhibited by sodium azide and by Pactamycin, an inhibitor of protein synthesis.", "contents": "Secretion of Ia antigens by a subpopulation of T cells which are Ly-1+, Ly-2-, and Ia-. It was found that Ia antignes are rapidly secreted by a subpopulation of splenic T lymphocytes which are nonadherent and which express the surface phenotype Ly-1+, Ly-2-, and Ia-. Secretion of the Ia antigens was a metabolically active process which was inhibited by sodium azide and by Pactamycin, an inhibitor of protein synthesis."}
{"id": "PMID:1085343", "title": "Interferon production in athymic nude mice.", "content": "Athymic (homozygous nude) mice of a non-inbred stock had relatively little antiviral activity in their serum compared with normal control mice at 4, 6 and 6.5 h after the intraperitoneal injection of Newcastle disease virus. The antiviral activity in the serum had the characteristics of interferon. At 10 h after injection and thereafter, the serum titres were comparable in nude and normal control mice. Exceptional nude mice with thymus-like tissue sometimes produced interferon more or less normally. Transfer of spleen cells from normal donor mice, but not from nude donors, led to increased serum interferon levels in nude recipient mice at 4 h after virus injection.", "contents": "Interferon production in athymic nude mice. Athymic (homozygous nude) mice of a non-inbred stock had relatively little antiviral activity in their serum compared with normal control mice at 4, 6 and 6.5 h after the intraperitoneal injection of Newcastle disease virus. The antiviral activity in the serum had the characteristics of interferon. At 10 h after injection and thereafter, the serum titres were comparable in nude and normal control mice. Exceptional nude mice with thymus-like tissue sometimes produced interferon more or less normally. Transfer of spleen cells from normal donor mice, but not from nude donors, led to increased serum interferon levels in nude recipient mice at 4 h after virus injection."}
{"id": "PMID:1085344", "title": "Changing to problem-oriented methods. Implementation in psychiatric institutions.", "content": "The so-called problem-oriented methods for organizing and recording clinical information offer many potential benefits to users in psychiatric institutions. Beyond the mechanical aspects of implementation, incorporating a problem-oriented approach into the practices of clinical teams entails conceptual and practical readjustments of considerable magnitude. Based on an 18-month study of eight psychiatric teams with diverse characteristics, the paper discusses rationales and priorities, as well as administrative and educational considerations in the conversion process. Such a process must be studied and understood in setting objectives and channeling resources, if outcomes are to match the expectations.", "contents": "Changing to problem-oriented methods. Implementation in psychiatric institutions. The so-called problem-oriented methods for organizing and recording clinical information offer many potential benefits to users in psychiatric institutions. Beyond the mechanical aspects of implementation, incorporating a problem-oriented approach into the practices of clinical teams entails conceptual and practical readjustments of considerable magnitude. Based on an 18-month study of eight psychiatric teams with diverse characteristics, the paper discusses rationales and priorities, as well as administrative and educational considerations in the conversion process. Such a process must be studied and understood in setting objectives and channeling resources, if outcomes are to match the expectations."}
{"id": "PMID:1085345", "title": "EEG, 5-HTP-metabolism and acupuncture.", "content": "Correlations between EEG-alterations and administration of 5-HT and 5-HTP in man are described. Age-dependence can be observed. Successful acupuncture produces EEG-alterations especially in younger patients as seen after exogene administration of 5-HT respectively 5-HTP i.v. Since biochemical indications for an increased 5-HT turnover during acupuncture of several acupuncture-points are evident further investigations should be carried out to enable reliable correlations.", "contents": "EEG, 5-HTP-metabolism and acupuncture. Correlations between EEG-alterations and administration of 5-HT and 5-HTP in man are described. Age-dependence can be observed. Successful acupuncture produces EEG-alterations especially in younger patients as seen after exogene administration of 5-HT respectively 5-HTP i.v. Since biochemical indications for an increased 5-HT turnover during acupuncture of several acupuncture-points are evident further investigations should be carried out to enable reliable correlations."}
{"id": "PMID:1085349", "title": "Simultaneous determination of eight vitamin D2 isomers by high-speed liquid chromatography.", "content": "The simultaneous determination of vitamin D2 and its isomers by high-speed liquid chromatography (HSLC) is described. Preferred operating conditions for the resolution and quantitation of vitamin D2 5,6-trans-vitamin D2, ergosterol, lumisterol2, isovitamin D2, isotachysterol2, tachysterol2, and previtamin D2 are as follows: column, \"Zorbax\" SIL (25 cm x 2.1 mm i.d.); pressure, 100-120 kg/cm2; temperature, ambient; detector, UV 254 nm; mobile phase, 0.15% methanol + 2% ether in pentane (for the simultaneous determination), 10% ether in hexane (for quantitation of the practical sets of isomers), or 55% CHCl3 (distilled) in pentane (for separation of isovitamin D2 and 5,6-trans-vitamin D2); sample size, 1 mul (greater than 1-5 ng); internal standard, p-cresol or alpha-naphthol. High-speed, good resolution, precise and accurate trace analysis, greater analysis flexibility, no necessity for preparing derivatives, and gentle operating conditions are main potential advantages of our method which has proved to be very efficient and surpasses all analytical procedures hitherto proposed.", "contents": "Simultaneous determination of eight vitamin D2 isomers by high-speed liquid chromatography. The simultaneous determination of vitamin D2 and its isomers by high-speed liquid chromatography (HSLC) is described. Preferred operating conditions for the resolution and quantitation of vitamin D2 5,6-trans-vitamin D2, ergosterol, lumisterol2, isovitamin D2, isotachysterol2, tachysterol2, and previtamin D2 are as follows: column, \"Zorbax\" SIL (25 cm x 2.1 mm i.d.); pressure, 100-120 kg/cm2; temperature, ambient; detector, UV 254 nm; mobile phase, 0.15% methanol + 2% ether in pentane (for the simultaneous determination), 10% ether in hexane (for quantitation of the practical sets of isomers), or 55% CHCl3 (distilled) in pentane (for separation of isovitamin D2 and 5,6-trans-vitamin D2); sample size, 1 mul (greater than 1-5 ng); internal standard, p-cresol or alpha-naphthol. High-speed, good resolution, precise and accurate trace analysis, greater analysis flexibility, no necessity for preparing derivatives, and gentle operating conditions are main potential advantages of our method which has proved to be very efficient and surpasses all analytical procedures hitherto proposed."}
{"id": "PMID:1085350", "title": "Simultaneous determination of eight vitamin D2 isomers by proton magnetic resonance spectroscopy.", "content": "Simultaneous determination of vitamin D2 and its isomers, with the aid of pmr employing a paramagnetic shift reagent Eu (dpm)3, is presented. Thus, a mixture of vitamin D2 isomers is acetylated and its pmr spectra are run with and without the addition of the shift reagent. Relative paramagnetic shift values (gamma rel) of the acetoxyl protons of the acetylated isomers are estimated for solutions in CCl4 against the reference acetoxyl signal of vitamin D2 acetate. Estimated values are as follows: vitamin D2 acetate = 100, 5,6-trans-vitamin D2 acetate = 71 +/- 1, ergosteryl acetate = 86 +/- 1, lumisteryl2 acetate = 123 +/- 3, isovitamin D2 acetate = 82, isotachysteryl2 acetate = 97, tachysteryl2 acetate = 100 (105 +/- 1 in C6D6), and previtamin D2 acetate = 80 +/- 2. On the basis of a dramatic shift of an acetoxyl signal and a constancy of each gamma rel, this method permits immediate identification of the isomers and provides valuable information on their stereochemistry. This technique also facilitates a simultaneous determination of these isomers in a mixture by estimating each signal area.", "contents": "Simultaneous determination of eight vitamin D2 isomers by proton magnetic resonance spectroscopy. Simultaneous determination of vitamin D2 and its isomers, with the aid of pmr employing a paramagnetic shift reagent Eu (dpm)3, is presented. Thus, a mixture of vitamin D2 isomers is acetylated and its pmr spectra are run with and without the addition of the shift reagent. Relative paramagnetic shift values (gamma rel) of the acetoxyl protons of the acetylated isomers are estimated for solutions in CCl4 against the reference acetoxyl signal of vitamin D2 acetate. Estimated values are as follows: vitamin D2 acetate = 100, 5,6-trans-vitamin D2 acetate = 71 +/- 1, ergosteryl acetate = 86 +/- 1, lumisteryl2 acetate = 123 +/- 3, isovitamin D2 acetate = 82, isotachysteryl2 acetate = 97, tachysteryl2 acetate = 100 (105 +/- 1 in C6D6), and previtamin D2 acetate = 80 +/- 2. On the basis of a dramatic shift of an acetoxyl signal and a constancy of each gamma rel, this method permits immediate identification of the isomers and provides valuable information on their stereochemistry. This technique also facilitates a simultaneous determination of these isomers in a mixture by estimating each signal area."}
{"id": "PMID:1085351", "title": "Antibodies to nucleic acids in congenital immune deficiency states.", "content": "The presence of antibodies to native DNA, single-stranded DNA, and double-stranded RNA was determined for 37 patients with selective IgA deficiency, 11 patients with Wiskott-Aldrich syndrome, seven patients with common variable agammaglobulinemia, 14 patients with ataxia telangiectasia, six patients with intestinal lymphangiectasia, and one patient with Nezelof syndrome. Of 37 patients with selective IgA deficiency, 11 had antibodies to at least one nucleic acid; six had antibodies to native DNA, seven had antibodies to single-stranded DNA, and four had antibodies to double-stranded RNA. The only other congenital immune deficiency disease studied in which antibodies to nucleic acids were found  was the Wiskott-Aldrich syndrome; in this group three of 11 patients had antibodies to native DNA. Retrospective analysis of our patients with SLE disclosed a 2.6% prevalence of IgA deficiency, a prevalence clearly higher than in the general population. These studies provide further evidence of the association between autoimmunity and abnormalities of IgA production and suggest a relationship between thymic-derived immune regulation and IgA production.", "contents": "Antibodies to nucleic acids in congenital immune deficiency states. The presence of antibodies to native DNA, single-stranded DNA, and double-stranded RNA was determined for 37 patients with selective IgA deficiency, 11 patients with Wiskott-Aldrich syndrome, seven patients with common variable agammaglobulinemia, 14 patients with ataxia telangiectasia, six patients with intestinal lymphangiectasia, and one patient with Nezelof syndrome. Of 37 patients with selective IgA deficiency, 11 had antibodies to at least one nucleic acid; six had antibodies to native DNA, seven had antibodies to single-stranded DNA, and four had antibodies to double-stranded RNA. The only other congenital immune deficiency disease studied in which antibodies to nucleic acids were found  was the Wiskott-Aldrich syndrome; in this group three of 11 patients had antibodies to native DNA. Retrospective analysis of our patients with SLE disclosed a 2.6% prevalence of IgA deficiency, a prevalence clearly higher than in the general population. These studies provide further evidence of the association between autoimmunity and abnormalities of IgA production and suggest a relationship between thymic-derived immune regulation and IgA production."}
{"id": "PMID:1085352", "title": "Neonatal hypoxia and pulmonary vasospasm: response to tolazoline.", "content": "Forty-six neonates with hypoxemia were treated with tolazoline, a pulmonary vasodilator, within the first two days of life. Eight of ten (80%) infants without apparent lung disease responded with a mean increase in PaO2 of 116 torr within one hour of beginning tolazoline infusions. One of the responding infants and two nonresponders died. Thirty-six additional infants with a variety of pulmonary disorders had severe hypoxemia which was refractory to mechanical ventilation. Twenty-one (58%) responded with a mean increase in PaO2 of 130 torr within one hour after beginning tolazoline and 13 (62%) of these survived. Fifteen patients had little or no improvement in PaO2 following tolazoline and only three (20%) of these infants survived. Responders could not be distinguished from nonresponders by clinical or laboratory features prior to therapy with tolazoline. Fourteen infants experienced complications possibly related to tolazoline.", "contents": "Neonatal hypoxia and pulmonary vasospasm: response to tolazoline. Forty-six neonates with hypoxemia were treated with tolazoline, a pulmonary vasodilator, within the first two days of life. Eight of ten (80%) infants without apparent lung disease responded with a mean increase in PaO2 of 116 torr within one hour of beginning tolazoline infusions. One of the responding infants and two nonresponders died. Thirty-six additional infants with a variety of pulmonary disorders had severe hypoxemia which was refractory to mechanical ventilation. Twenty-one (58%) responded with a mean increase in PaO2 of 130 torr within one hour after beginning tolazoline and 13 (62%) of these survived. Fifteen patients had little or no improvement in PaO2 following tolazoline and only three (20%) of these infants survived. Responders could not be distinguished from nonresponders by clinical or laboratory features prior to therapy with tolazoline. Fourteen infants experienced complications possibly related to tolazoline."}
{"id": "PMID:1085348", "title": "Remarks concerning 102 watersoluble contrast ventriculographies.", "content": "The Authors report their experience in using water-soluble contrast medium in ventriculographies having carried out 102 ventriculographies partly with Dimer X and partly with \"Uromiro 300\". Their conclusions are practically similar to those already reported by the many Authors who have written on this subject: 1) facility of examination particularly in urgent cases; 2) excellent neuroradiological definition of the cases; 3) possibility of obtaining a selective ventriculography; 4) indication especially in space-occupying lesions of the posterior cranial contrast medium; 6) possibility of reducing complication rate by carefully carrying out the examination and by avoiding the use of excessive quantities of contrast. In particular the Authors describe some useful methods for obtaining better technical results from the examination and reducing the complication rate.", "contents": "Remarks concerning 102 watersoluble contrast ventriculographies. The Authors report their experience in using water-soluble contrast medium in ventriculographies having carried out 102 ventriculographies partly with Dimer X and partly with \"Uromiro 300\". Their conclusions are practically similar to those already reported by the many Authors who have written on this subject: 1) facility of examination particularly in urgent cases; 2) excellent neuroradiological definition of the cases; 3) possibility of obtaining a selective ventriculography; 4) indication especially in space-occupying lesions of the posterior cranial contrast medium; 6) possibility of reducing complication rate by carefully carrying out the examination and by avoiding the use of excessive quantities of contrast. In particular the Authors describe some useful methods for obtaining better technical results from the examination and reducing the complication rate."}
{"id": "PMID:1085354", "title": "A voltage-clamp study of the effect of two lidocaine derivatives on the time course of end-plate currents.", "content": "1. Voltage-clamped end-plate currents (e.p.c.s) have been studied in the glycerol-treated Rana pipiens sartorius nerve-muscle preparations in normal Ringer solution and in the presence of QX-222 and QX-314. 2. Both QX-222 and QX-314, the trimethyl and triethyl analogues, respectively, of lidocaine, greatly modify end-plate current kinetics. The altered e.p.c.s still show a true reversal potential, which is essentially the same as the reversal potential before drug treatment. The time course of the altered end-plate currents varies with both membrane potential and drug concentration. 3. In the presence of 0-1-1-0 mM QX-222, end-plate currents decay as the sum of three exponentials: I(t) =I1(0)e-k1t+I2(0)e-k2t+I3(0)e-k3t, where the subscipts 1, 2 and 3 refer to the rapidly, intermediately, and slowly decaying components, respectively. Both the amplitudes, Ij(0), and the decay rates, kj, depend upon membrane potential. 4. Hyperpolarization increases the relative size of the first and third components, i.e. I1(0) and I3(0) increase relative to I2(0). Depolarization increases the relative size of the second component. 5. Hyperpolarization causes a decrease in the decay rates k2 and k3 and causes a slight increase in the decay rate k1. Dependence of the three decay rates on membrane potential is well described by: kj=bjeajv. 6. The Q10 of each of the kj is about 3. 7. Raising QX-222 concentration, at any given membrane potential, augments I1(0) and I3(0) at the expense of I2(0). Raising concentration increases k1 and decreases k3; their voltage-dependence is little affected. 8. At all QX-222 concentrations tested the decay rate k2 is nearly the same as the decay rate of a normal e.p.c. recorded at an equivalent holding potential from the same fibre before drug exposure. 9. End-plate currents in the presence of 0-1 mM-QX-314 show a \"major\" or rapidly decaying phase and a very small, slowly decaying phase or \"tail\", but no intermediate component. Only the major component is discernible for end-plate currents in 0-5 mM-QX-314. 10. Voltage- and concentration-dependence of the decay rate of the major component in QX-314 is similar to k1 and QX-222. Voltage-dependence of the tails decay rate appears to be similar to k3. It is hypothesized that the second component in QX-222 represents currents of unaltered or normal conductance kinetics, and that the first and third components in QX-222, as well as the major component and tail in QX-314, represent current of \"QX-altered conductance kinetics\".", "contents": "A voltage-clamp study of the effect of two lidocaine derivatives on the time course of end-plate currents. 1. Voltage-clamped end-plate currents (e.p.c.s) have been studied in the glycerol-treated Rana pipiens sartorius nerve-muscle preparations in normal Ringer solution and in the presence of QX-222 and QX-314. 2. Both QX-222 and QX-314, the trimethyl and triethyl analogues, respectively, of lidocaine, greatly modify end-plate current kinetics. The altered e.p.c.s still show a true reversal potential, which is essentially the same as the reversal potential before drug treatment. The time course of the altered end-plate currents varies with both membrane potential and drug concentration. 3. In the presence of 0-1-1-0 mM QX-222, end-plate currents decay as the sum of three exponentials: I(t) =I1(0)e-k1t+I2(0)e-k2t+I3(0)e-k3t, where the subscipts 1, 2 and 3 refer to the rapidly, intermediately, and slowly decaying components, respectively. Both the amplitudes, Ij(0), and the decay rates, kj, depend upon membrane potential. 4. Hyperpolarization increases the relative size of the first and third components, i.e. I1(0) and I3(0) increase relative to I2(0). Depolarization increases the relative size of the second component. 5. Hyperpolarization causes a decrease in the decay rates k2 and k3 and causes a slight increase in the decay rate k1. Dependence of the three decay rates on membrane potential is well described by: kj=bjeajv. 6. The Q10 of each of the kj is about 3. 7. Raising QX-222 concentration, at any given membrane potential, augments I1(0) and I3(0) at the expense of I2(0). Raising concentration increases k1 and decreases k3; their voltage-dependence is little affected. 8. At all QX-222 concentrations tested the decay rate k2 is nearly the same as the decay rate of a normal e.p.c. recorded at an equivalent holding potential from the same fibre before drug exposure. 9. End-plate currents in the presence of 0-1 mM-QX-314 show a \"major\" or rapidly decaying phase and a very small, slowly decaying phase or \"tail\", but no intermediate component. Only the major component is discernible for end-plate currents in 0-5 mM-QX-314. 10. Voltage- and concentration-dependence of the decay rate of the major component in QX-314 is similar to k1 and QX-222. Voltage-dependence of the tails decay rate appears to be similar to k3. It is hypothesized that the second component in QX-222 represents currents of unaltered or normal conductance kinetics, and that the first and third components in QX-222, as well as the major component and tail in QX-314, represent current of \"QX-altered conductance kinetics\"."}
{"id": "PMID:1085358", "title": "Stimulation of the sodium pump by azide and high internal sodium: changes in the number of pumping sites and turnover rate.", "content": "1. The effects of 5 mM azide on [3H]ouabain uptake and 22Na efflux were determined. Both glycoside uptake and 22Na efflux were enhanced by azide. 2. Azide stimulated the Na pump in muscles whose pumping sites had been inhibited by ouabain and then transferred to a glycoside-free solution. This stimulation was observed before detecting any recovery of the initial pumping activity. 3. When both the resting and the azide-stimulated 22Na efflux had been blocked by ouabain, an additional exposure to azide, in a ouabain-free solution, had no further effects on 22Na efflux. 4. It is concluded that the increase in Na pumping caused by azide is due in part to an increase in the number of pumping sites. 5. [3H]ouabain binding was measured in muscles with different intracellular alkali cation concentrations. Variations in [Na]i from 15 up to 50 mM did not significantly affect the amount of glycoside bound. A substantial increase in binding occurred when [Na]i reached 70 mM. 6. It is proposed that the increase in Na extrusion that occurs during the recovery of Na loaded muscles mostly results from an increased turnover rate of the pump rather than from an increase in number of pumping sites.", "contents": "Stimulation of the sodium pump by azide and high internal sodium: changes in the number of pumping sites and turnover rate. 1. The effects of 5 mM azide on [3H]ouabain uptake and 22Na efflux were determined. Both glycoside uptake and 22Na efflux were enhanced by azide. 2. Azide stimulated the Na pump in muscles whose pumping sites had been inhibited by ouabain and then transferred to a glycoside-free solution. This stimulation was observed before detecting any recovery of the initial pumping activity. 3. When both the resting and the azide-stimulated 22Na efflux had been blocked by ouabain, an additional exposure to azide, in a ouabain-free solution, had no further effects on 22Na efflux. 4. It is concluded that the increase in Na pumping caused by azide is due in part to an increase in the number of pumping sites. 5. [3H]ouabain binding was measured in muscles with different intracellular alkali cation concentrations. Variations in [Na]i from 15 up to 50 mM did not significantly affect the amount of glycoside bound. A substantial increase in binding occurred when [Na]i reached 70 mM. 6. It is proposed that the increase in Na extrusion that occurs during the recovery of Na loaded muscles mostly results from an increased turnover rate of the pump rather than from an increase in number of pumping sites."}
{"id": "PMID:1085359", "title": "The effects of very low external calcium and sodium concentrations on cardiac contractile strength and calcium-sodium antagonism.", "content": "1. The tension levels of the contractures induced in frog atrial trabeculae by reduction of the bathing Na concentration have been investigated over a wide range of [Na+]o and [Ca2+]o, making use of EGTA to buffer [Ca2+]o under 10(-5)M. 2. The relationship between tension and the quotient [Ca2+]o/[Na+]2o is only an apparent one, and holds approximately for Na concentrations as low as 1-5 mM. 3. However, for low Na concentrations tension depends on the absolute values of both [Ca2+]o and [Na+]o. 4. Extracellular Ca is the essential factor in tension production. The results indicate an apparent threshold for tension near 5 X 10(-8) M-[Ca2+]o. 5. It can be shown that, under the present experimental conditions, the Na concentration around the cells must be lower than about 0-1 mM in 'zero' Na+ solutions. 6. It is shown that the over-all process leading to tension production can be explained in terms of a reaction involving two Ca ions. 7. To account for the results, a modification of the reaction scheme put forward by L\u00fcttgau & Niedergerke (1958) is proposed, involving a competitive action between Ca and Na ions at a free receptor site. 8. To account for the scheme, an example of a mechanism involving both internal and external Na and Ca concentrations is considered.", "contents": "The effects of very low external calcium and sodium concentrations on cardiac contractile strength and calcium-sodium antagonism. 1. The tension levels of the contractures induced in frog atrial trabeculae by reduction of the bathing Na concentration have been investigated over a wide range of [Na+]o and [Ca2+]o, making use of EGTA to buffer [Ca2+]o under 10(-5)M. 2. The relationship between tension and the quotient [Ca2+]o/[Na+]2o is only an apparent one, and holds approximately for Na concentrations as low as 1-5 mM. 3. However, for low Na concentrations tension depends on the absolute values of both [Ca2+]o and [Na+]o. 4. Extracellular Ca is the essential factor in tension production. The results indicate an apparent threshold for tension near 5 X 10(-8) M-[Ca2+]o. 5. It can be shown that, under the present experimental conditions, the Na concentration around the cells must be lower than about 0-1 mM in 'zero' Na+ solutions. 6. It is shown that the over-all process leading to tension production can be explained in terms of a reaction involving two Ca ions. 7. To account for the results, a modification of the reaction scheme put forward by L\u00fcttgau & Niedergerke (1958) is proposed, involving a competitive action between Ca and Na ions at a free receptor site. 8. To account for the scheme, an example of a mechanism involving both internal and external Na and Ca concentrations is considered."}
{"id": "PMID:1085360", "title": "The formation of metarhodospin380 in the retinal rods of the frog.", "content": "1. The formation of metarhodopsin380 (metarhodopsin II) was studied in isolated frog retinas exposed to intense flashes of 120 mus duration. 2. A rapid increase in absorbance at 375 nm during the flash was followed by a slower absorbance increase in the subsequent dark period. The slower increase showed virtual completion after 5 ms. 3. The fast absorbance increase during the flash was due to the formation of metarhodopsin478. The rate of this reaction was dependent on the time course of the flash and on the decay rate of lumirhodopsin. 4. Kinetic analysis indicates that three consecutive reactions occur: the light-controlled formation of lumirhodopsin, its first-order decay to metarhodopsin478 and the conversion of metarhodopsin478 into metarhodopsin380. At 21 degrees C, the decay constants were 2 X 10(4) S-1 (lumirhodopsin) and 1 X 10(3) S-1 (metarhodopsin478), respectively.", "contents": "The formation of metarhodospin380 in the retinal rods of the frog. 1. The formation of metarhodopsin380 (metarhodopsin II) was studied in isolated frog retinas exposed to intense flashes of 120 mus duration. 2. A rapid increase in absorbance at 375 nm during the flash was followed by a slower absorbance increase in the subsequent dark period. The slower increase showed virtual completion after 5 ms. 3. The fast absorbance increase during the flash was due to the formation of metarhodopsin478. The rate of this reaction was dependent on the time course of the flash and on the decay rate of lumirhodopsin. 4. Kinetic analysis indicates that three consecutive reactions occur: the light-controlled formation of lumirhodopsin, its first-order decay to metarhodopsin478 and the conversion of metarhodopsin478 into metarhodopsin380. At 21 degrees C, the decay constants were 2 X 10(4) S-1 (lumirhodopsin) and 1 X 10(3) S-1 (metarhodopsin478), respectively."}
{"id": "PMID:1085366", "title": "The influence of drugs on the kinin-forming system in relation to pregnancy and parturition in the rat.", "content": "The duration of normal gestation and parturition in the rat can be changed by treatment with drugs which alter the equilibrium of the kallikrein-kinin system. The kallikrein inhibitor, aprotinin, when given from Days 19-22 of pregnancy prolongs gestation. Treatment with aprotinin from Days 20-22 of pregnancy prolongs the parturient process, as does a single dose given on the morning of Day 22. Kallikrein, when administered from Days 19-22 of pregnancy, results in a prolongation of gestation and abolishes the pre-parturient behaviour ('labour'). Parturition is prolonged and many fetuses are stillborn. Soya bean trypsin inhibitor when given from Days 19-22 of pregnancy delays and prolongs parturition; maternal haemorrhage occurs during birth and many fetuses are born dead or are abandoned at birth. It is suggested that the kallikrein-kinin system plays a functional role in the normal process of parturition in the rat.", "contents": "The influence of drugs on the kinin-forming system in relation to pregnancy and parturition in the rat. The duration of normal gestation and parturition in the rat can be changed by treatment with drugs which alter the equilibrium of the kallikrein-kinin system. The kallikrein inhibitor, aprotinin, when given from Days 19-22 of pregnancy prolongs gestation. Treatment with aprotinin from Days 20-22 of pregnancy prolongs the parturient process, as does a single dose given on the morning of Day 22. Kallikrein, when administered from Days 19-22 of pregnancy, results in a prolongation of gestation and abolishes the pre-parturient behaviour ('labour'). Parturition is prolonged and many fetuses are stillborn. Soya bean trypsin inhibitor when given from Days 19-22 of pregnancy delays and prolongs parturition; maternal haemorrhage occurs during birth and many fetuses are born dead or are abandoned at birth. It is suggested that the kallikrein-kinin system plays a functional role in the normal process of parturition in the rat."}
{"id": "PMID:1085369", "title": "Probable common origin of a hereditary fundus dystrophy (Sorsby's familial pseudoinflammatory macular dystrophy) in an English and Australian family.", "content": "A genealogical link was established six generations back between a family living in England and Australia, and one of the families reported originally by Sorsby et al (1949) as suffering from autosomal dominant inflammatory macular dystrophy (fundus dystrophy). The onset--in the fifth decade of life--and the progress of the condition, which usually ends in blindness, has been observed in a number of patients and the prodromal development of a colour vision deficiency in some of them confirmed. This defect is fundamentally different from the X-linked colour vision defects and merits further investigation.", "contents": "Probable common origin of a hereditary fundus dystrophy (Sorsby's familial pseudoinflammatory macular dystrophy) in an English and Australian family. A genealogical link was established six generations back between a family living in England and Australia, and one of the families reported originally by Sorsby et al (1949) as suffering from autosomal dominant inflammatory macular dystrophy (fundus dystrophy). The onset--in the fifth decade of life--and the progress of the condition, which usually ends in blindness, has been observed in a number of patients and the prodromal development of a colour vision deficiency in some of them confirmed. This defect is fundamentally different from the X-linked colour vision defects and merits further investigation."}
{"id": "PMID:1085370", "title": "X mapping in man: evidence against direct measurable linkage between ocular albinism and deutan colour blindness.", "content": "A Newfoundland kindred in which ocular albinism and deutan colour blindness are segregating provides strong evidence against the loci for these two X-borne characters being within direct measurable distance of each other.", "contents": "X mapping in man: evidence against direct measurable linkage between ocular albinism and deutan colour blindness. A Newfoundland kindred in which ocular albinism and deutan colour blindness are segregating provides strong evidence against the loci for these two X-borne characters being within direct measurable distance of each other."}
{"id": "PMID:1085375", "title": "Classification criteria for systemic lupus erythematosus. Frequency in normal patients.", "content": "One hundred healthy women were examined prospectively during their annual physical examination for any of the preliminary criteria of systemic lupus erythematosus (SLE). No patient had more than three of the criteria; 50 (50%) had none. The preliminary criteria were effective in excluding normal patients. Three of 19 patients taking oral contraceptives had serum antinuclear antibody (ANA), whereas only one of 81 non-pill users had serum ANA (P less than .05). None of these patients with positive ANA had clinical evidence of SLE.", "contents": "Classification criteria for systemic lupus erythematosus. Frequency in normal patients. One hundred healthy women were examined prospectively during their annual physical examination for any of the preliminary criteria of systemic lupus erythematosus (SLE). No patient had more than three of the criteria; 50 (50%) had none. The preliminary criteria were effective in excluding normal patients. Three of 19 patients taking oral contraceptives had serum antinuclear antibody (ANA), whereas only one of 81 non-pill users had serum ANA (P less than .05). None of these patients with positive ANA had clinical evidence of SLE."}
{"id": "PMID:1085379", "title": "Heterotransplantation of cell lines derived from human malignant neoplasms into golden hamsters treated with antithymocyte serum.", "content": "Thirteen cell lines of tissue culture originated from human malignant neoplasms were transplanted into cheek pouches of adult golden hamsters treated with anti-hamster thymocyte serum. In eleven cell lines, transplantation tests with 3.5 X 10(6) to 6.8 X 10(6) cells resulted in neoplastic growth within 4 weeks. In other two cell lines including one from neuroblastoma and another from Burkitt's lymphoma, the inoculum size had to be raised to 11.8 X 10(6) and 12.2 X 10(6) cells to get tumor growth, respectively. The histological appearance of those tumors was consistent with that of the individual primary neoplasms despite of their long in vitro histories. Anaplasia grading of the tumors appeared not to be correlated with the frequency of take.", "contents": "Heterotransplantation of cell lines derived from human malignant neoplasms into golden hamsters treated with antithymocyte serum. Thirteen cell lines of tissue culture originated from human malignant neoplasms were transplanted into cheek pouches of adult golden hamsters treated with anti-hamster thymocyte serum. In eleven cell lines, transplantation tests with 3.5 X 10(6) to 6.8 X 10(6) cells resulted in neoplastic growth within 4 weeks. In other two cell lines including one from neuroblastoma and another from Burkitt's lymphoma, the inoculum size had to be raised to 11.8 X 10(6) and 12.2 X 10(6) cells to get tumor growth, respectively. The histological appearance of those tumors was consistent with that of the individual primary neoplasms despite of their long in vitro histories. Anaplasia grading of the tumors appeared not to be correlated with the frequency of take."}
{"id": "PMID:1085380", "title": "Stimulation of lymphocytes of patients administered with a trypsin inhibitor, Trasylol (basic pancreatic trypsin inhibitor pharmaceutical), in vitro with BPTI and other several stimulants.", "content": "Distinguished lymphocytes stimulation was observed in vitro with one out of four patients received Trasylol therapy. The peak response of DNA synthesis was shown to occur at 100 mug/tube of basic pancreatic trypsin inhibitor (BPTI) itself, about 6 days after addition of BPTI. Reactivity (3H-thymidine incorporation) of lymphocytes of patients after the therapy, to specific antigen, ie., purified protein derivative (PPD) or Bordetella pertussis organisms in vitro, was markedly depressed. On the other hand, that to non-specific mitogen, phytohaemagglutinin-p of pokeweed mitogen, was a little reduced, or rather enhanced after the therapy. Conversly, lymphocytes of an originally tuberculin negative patient were lead to be stimulated with PPD in vitro, after skin test with PPD and Trasylol therapy. These various immune responses including drug-induced exanthemas of patients received the therapy, are discussed in this report, in comparison with immune responses of guinea pigs sensitized with BPTI, immune responses of animals in microorganism infections and the contrary immunological effects of Trasylol already reported.", "contents": "Stimulation of lymphocytes of patients administered with a trypsin inhibitor, Trasylol (basic pancreatic trypsin inhibitor pharmaceutical), in vitro with BPTI and other several stimulants. Distinguished lymphocytes stimulation was observed in vitro with one out of four patients received Trasylol therapy. The peak response of DNA synthesis was shown to occur at 100 mug/tube of basic pancreatic trypsin inhibitor (BPTI) itself, about 6 days after addition of BPTI. Reactivity (3H-thymidine incorporation) of lymphocytes of patients after the therapy, to specific antigen, ie., purified protein derivative (PPD) or Bordetella pertussis organisms in vitro, was markedly depressed. On the other hand, that to non-specific mitogen, phytohaemagglutinin-p of pokeweed mitogen, was a little reduced, or rather enhanced after the therapy. Conversly, lymphocytes of an originally tuberculin negative patient were lead to be stimulated with PPD in vitro, after skin test with PPD and Trasylol therapy. These various immune responses including drug-induced exanthemas of patients received the therapy, are discussed in this report, in comparison with immune responses of guinea pigs sensitized with BPTI, immune responses of animals in microorganism infections and the contrary immunological effects of Trasylol already reported."}
{"id": "PMID:1085382", "title": "Clinical significance of bradykinin liberation during cardiopulmonary bypass and its prevention by a kallikrein inhibitor.", "content": "Activation of the kinin system and effects  of Trasylol (Bayer, A.G. Lebukusen, West Germany), a kallikrein inhibitor, were investigated on 52 patients during hemodilutional cardiopulmonary bypass (CPB). Immediately after the start of CPB, neither elevation of bradykinin nor reduction of plasma kininogen (KGN: a precursor of bradykinin) were observed. During CPB, bradykinin level in the blood was markedly elevated, correlating with the significant decrease of kininogen (p less than 0.001). The longer the CPB time, the more marked the reduction of KGN. In the cases requiring over 60 minutes of CPB, the amounts of bradykinin released (4.6-18.0ng/ml) were sufficient to increase capillary permeability as well as peripheral vasodilatation. As shown by the sufnificant increase of hematocrit (p less than 0.005) and the extreme reduction of vascular resistance found at the end of CPB in the prolonged cases. Infusion of Trasylol into the extracorporeal circuit actually prevented the reduction of kininogen and the increase of hematocrit as well as the extreme decrease of vascular resistance in the cases of over 60 minutes CPB. These results clearly point out that Trasylol is beneficial for the prevention of bradykinin liberation and capillary permeability increase and for the maintenance of optimum peripheral vascular tone during CPB. Furthermore, the significance of these findings with regards to complications during and after prolonged CPB was discussed.", "contents": "Clinical significance of bradykinin liberation during cardiopulmonary bypass and its prevention by a kallikrein inhibitor. Activation of the kinin system and effects  of Trasylol (Bayer, A.G. Lebukusen, West Germany), a kallikrein inhibitor, were investigated on 52 patients during hemodilutional cardiopulmonary bypass (CPB). Immediately after the start of CPB, neither elevation of bradykinin nor reduction of plasma kininogen (KGN: a precursor of bradykinin) were observed. During CPB, bradykinin level in the blood was markedly elevated, correlating with the significant decrease of kininogen (p less than 0.001). The longer the CPB time, the more marked the reduction of KGN. In the cases requiring over 60 minutes of CPB, the amounts of bradykinin released (4.6-18.0ng/ml) were sufficient to increase capillary permeability as well as peripheral vasodilatation. As shown by the sufnificant increase of hematocrit (p less than 0.005) and the extreme reduction of vascular resistance found at the end of CPB in the prolonged cases. Infusion of Trasylol into the extracorporeal circuit actually prevented the reduction of kininogen and the increase of hematocrit as well as the extreme decrease of vascular resistance in the cases of over 60 minutes CPB. These results clearly point out that Trasylol is beneficial for the prevention of bradykinin liberation and capillary permeability increase and for the maintenance of optimum peripheral vascular tone during CPB. Furthermore, the significance of these findings with regards to complications during and after prolonged CPB was discussed."}
{"id": "PMID:1085383", "title": "[On secondary endoepithelial dystrophy of the cornea due to non-magnetic anterior chamber foreign body (author's transl)].", "content": "Secondary endoepithelial dystrophy of the cornea can be uncharacteristic and lead to misdiagnoses. A foreign body in the anterior chamber producing an endothelial dystrophy is often recognized late, or not at all. Clearing the cornea with glycerin facilitates the gonioscopic examination. Foreign bodies found in the anterior chamber following war wounds of traffic accidents were most commonly glass, quartz or stone. After removal of the foreign body, the endothelial dystrophy can heal spontaneously. A perforating keratoplasty of large diameter is indicated if the dystrophy persists or increases. The results are reported.", "contents": "[On secondary endoepithelial dystrophy of the cornea due to non-magnetic anterior chamber foreign body (author's transl)]. Secondary endoepithelial dystrophy of the cornea can be uncharacteristic and lead to misdiagnoses. A foreign body in the anterior chamber producing an endothelial dystrophy is often recognized late, or not at all. Clearing the cornea with glycerin facilitates the gonioscopic examination. Foreign bodies found in the anterior chamber following war wounds of traffic accidents were most commonly glass, quartz or stone. After removal of the foreign body, the endothelial dystrophy can heal spontaneously. A perforating keratoplasty of large diameter is indicated if the dystrophy persists or increases. The results are reported."}
{"id": "PMID:1085385", "title": "Spontaneous eosinophil-erythrocyte rosette formation.", "content": "Eosinophils of dogs form rosettes with washed human erythrocytes in a manner very similar to that of thymus-derived (T) lymphocytes. Identification of rosette-forming eosinophils was made by both light and electron microscopic study of eosinophils infiltrating into the canine transmissible venereal sarcoma.", "contents": "Spontaneous eosinophil-erythrocyte rosette formation. Eosinophils of dogs form rosettes with washed human erythrocytes in a manner very similar to that of thymus-derived (T) lymphocytes. Identification of rosette-forming eosinophils was made by both light and electron microscopic study of eosinophils infiltrating into the canine transmissible venereal sarcoma."}
{"id": "PMID:1085389", "title": "A case of fatal fat embolism syndrome following cardiopulmonary bypass.", "content": "A case of fat embolism syndrome following median sternotomy and cardiopulmonary bypass, with fatal outcome, is described. This syndrome is present subclinically in the majority of these surgical procedures. The etiology and possible mechanisms of production are discussed; management is empirical and aimed at maintaining the function of embolized microvascular endothelium.", "contents": "A case of fatal fat embolism syndrome following cardiopulmonary bypass. A case of fat embolism syndrome following median sternotomy and cardiopulmonary bypass, with fatal outcome, is described. This syndrome is present subclinically in the majority of these surgical procedures. The etiology and possible mechanisms of production are discussed; management is empirical and aimed at maintaining the function of embolized microvascular endothelium."}
{"id": "PMID:1085390", "title": "The assessment of function of left ventricle and patency of aorta-coronary bypass after operation. A study of systolic time intervals.", "content": "The effect of aorta-coronary bypass surgery on left ventricular function was evaluated in 39 patients by cardiac catheterization and recording of the systolic time intervals (STI) before and an average of 4.5 months following myocardial revascularization. All patients were in sinus rhythm, had normal QRS intervals on the electrocardiogram, and had taken no medication for at least 3 weeks prior to the study. The STI was unchanged postoperatively in patients with the following characteristics: freedom from angina; heart rate not more than 15 beats greater than the preoperative level; all grafts patent; and no progression of disease in coronary arteries. Deterioration in left ventricular function after operation as represented by shortened left ventricular ejection time, (LVET) prolonged pre-ejection period (PEP), and increased ratio of PEP/LVET was found in patients with the following characteristics: persistent angina; heart rate more than 14 beats faster than the preoperative level; occlusion of any grafts; progression of disease in the coronary arteries; evidence for postoperative myocardial infarction. Worsening of left ventricular performance as determined by STI was more apparent in patients with occluded grafts and no collateral circulation than in those with occluded grafts and collateral circulation which may have offered protection against ischemia. These investigations supported the use of STI in assessing changes in left ventricular function after coronary bypass and, by inference, in assessing the status of the graft.", "contents": "The assessment of function of left ventricle and patency of aorta-coronary bypass after operation. A study of systolic time intervals. The effect of aorta-coronary bypass surgery on left ventricular function was evaluated in 39 patients by cardiac catheterization and recording of the systolic time intervals (STI) before and an average of 4.5 months following myocardial revascularization. All patients were in sinus rhythm, had normal QRS intervals on the electrocardiogram, and had taken no medication for at least 3 weeks prior to the study. The STI was unchanged postoperatively in patients with the following characteristics: freedom from angina; heart rate not more than 15 beats greater than the preoperative level; all grafts patent; and no progression of disease in coronary arteries. Deterioration in left ventricular function after operation as represented by shortened left ventricular ejection time, (LVET) prolonged pre-ejection period (PEP), and increased ratio of PEP/LVET was found in patients with the following characteristics: persistent angina; heart rate more than 14 beats faster than the preoperative level; occlusion of any grafts; progression of disease in the coronary arteries; evidence for postoperative myocardial infarction. Worsening of left ventricular performance as determined by STI was more apparent in patients with occluded grafts and no collateral circulation than in those with occluded grafts and collateral circulation which may have offered protection against ischemia. These investigations supported the use of STI in assessing changes in left ventricular function after coronary bypass and, by inference, in assessing the status of the graft."}
{"id": "PMID:1085391", "title": "Tension on coronary bypass conduits. A neglected cause of real or potential obstruction of saphenous vein grafts.", "content": "Of 41 saphenous vein aorta-coronary bypass grafts from 21 patients, abnormal graft tension was judged to be present at necropsy in 6 grafts (15 per cent) from 5 patients (24 per cent). Five (33 per cent) of 15 grafts placed to the right coronary system and one (3 per cent) of 26 grafts to the left coronary system were under excess tension. The abnormal tension appeared to result from the insertion of grafts too short to accommodate normal or abnormal distention of the right atrium and ventricle (graft to distal right coronary or posterior descending coronary artery) or of the pulmonary trunk (graft to left coronary system) after discontinuation of cardiopulmonary bypass or development of acute congestive cardiac failure.", "contents": "Tension on coronary bypass conduits. A neglected cause of real or potential obstruction of saphenous vein grafts. Of 41 saphenous vein aorta-coronary bypass grafts from 21 patients, abnormal graft tension was judged to be present at necropsy in 6 grafts (15 per cent) from 5 patients (24 per cent). Five (33 per cent) of 15 grafts placed to the right coronary system and one (3 per cent) of 26 grafts to the left coronary system were under excess tension. The abnormal tension appeared to result from the insertion of grafts too short to accommodate normal or abnormal distention of the right atrium and ventricle (graft to distal right coronary or posterior descending coronary artery) or of the pulmonary trunk (graft to left coronary system) after discontinuation of cardiopulmonary bypass or development of acute congestive cardiac failure."}
{"id": "PMID:1085392", "title": "Pulmonary involvement by histiocytosis X in the pediatric age group.", "content": "The lungs may be involved by histiocytosis X either as part of a generalized disease or as a separate entity, primary pulmonary histiocytosis X. The former is much more frequent in the pediatric age group, and although it occurs primarily in those who are quite sick with widely disseminated disease, the lung involvement rarely caused symptoms and almost invariably clears. By contrast, primary pulmonary histiocytosis X is mainly seen in young adult males and frequently leads to severe disability. Twelve patients less than 20 years old with lung involvement by histiocytosis X are presented.", "contents": "Pulmonary involvement by histiocytosis X in the pediatric age group. The lungs may be involved by histiocytosis X either as part of a generalized disease or as a separate entity, primary pulmonary histiocytosis X. The former is much more frequent in the pediatric age group, and although it occurs primarily in those who are quite sick with widely disseminated disease, the lung involvement rarely caused symptoms and almost invariably clears. By contrast, primary pulmonary histiocytosis X is mainly seen in young adult males and frequently leads to severe disability. Twelve patients less than 20 years old with lung involvement by histiocytosis X are presented."}
{"id": "PMID:1085393", "title": "Human cochlear changes in noise induced hearing loss.", "content": "The temporal bone histopathological findings in 14 ears with noise induced hearing loss are presented. The morphological changes consist mainly of hair cell loss, which is more severe in the 9 mm to 13 mm region of the cochlear duct. Within the area of maximum hair cell loss, there is a greater loss of outer hair cells than of inner hair cells. There is a good correlation between the permanent auditory threshold shifts and the spatial location of the sensory lesion according to the anatomical frequency scale.", "contents": "Human cochlear changes in noise induced hearing loss. The temporal bone histopathological findings in 14 ears with noise induced hearing loss are presented. The morphological changes consist mainly of hair cell loss, which is more severe in the 9 mm to 13 mm region of the cochlear duct. Within the area of maximum hair cell loss, there is a greater loss of outer hair cells than of inner hair cells. There is a good correlation between the permanent auditory threshold shifts and the spatial location of the sensory lesion according to the anatomical frequency scale."}
{"id": "PMID:1085402", "title": "Analgesic nephropathy.", "content": "Analgesic nephropathy is part of a wider clinical syndrome associated with the abuse of APC compounds, that is, a minimum total intake of 2 kg of aspirin or phenacetin. Ischaemic heart disease and premature aging are newly recognized aspects of the analgesic syndrome. The diagnosis of analgesic nephropathy can be made precisely by the radiological demonstration of renal papillary necrosis. The most important aspect of management of established analgesic nephropathy and renal insufficency is total avoidance of all non-steroid antiinflammatory agents and this is commonly associated with stabilization or improvement in renal function. In the APC mixture, aspirin appears to be the major nephrotoxic agent while phenacetin and paracetamol play a secondary and synergistic role in the nephrotoxicity.", "contents": "Analgesic nephropathy. Analgesic nephropathy is part of a wider clinical syndrome associated with the abuse of APC compounds, that is, a minimum total intake of 2 kg of aspirin or phenacetin. Ischaemic heart disease and premature aging are newly recognized aspects of the analgesic syndrome. The diagnosis of analgesic nephropathy can be made precisely by the radiological demonstration of renal papillary necrosis. The most important aspect of management of established analgesic nephropathy and renal insufficency is total avoidance of all non-steroid antiinflammatory agents and this is commonly associated with stabilization or improvement in renal function. In the APC mixture, aspirin appears to be the major nephrotoxic agent while phenacetin and paracetamol play a secondary and synergistic role in the nephrotoxicity."}
{"id": "PMID:1085404", "title": "Methotrexate megadosage followed by folate rescue. I. A study in clearance patterns.", "content": "Plasma clearance rates of methotrexate  (MTX) have been determined in five patients given an infusion of 3,000 mg of MTX over six hours, followed by folate rescue 24 hours after the commencement of infusion. Results presented indicate that maintenance of high MTX concentrations and potential toxicity could not be related to age, body weight or surface area.", "contents": "Methotrexate megadosage followed by folate rescue. I. A study in clearance patterns. Plasma clearance rates of methotrexate  (MTX) have been determined in five patients given an infusion of 3,000 mg of MTX over six hours, followed by folate rescue 24 hours after the commencement of infusion. Results presented indicate that maintenance of high MTX concentrations and potential toxicity could not be related to age, body weight or surface area."}
{"id": "PMID:1085405", "title": "Localized tissue reduction.", "content": "A faradic muscle  stimulator (the Hawkins EBA machine) for localized tissue reduction is described. Forty patients undergoing a course of treatment with the machine all lost significant amounts of weight. No side effects were noted.", "contents": "Localized tissue reduction. A faradic muscle  stimulator (the Hawkins EBA machine) for localized tissue reduction is described. Forty patients undergoing a course of treatment with the machine all lost significant amounts of weight. No side effects were noted."}
{"id": "PMID:1085406", "title": "Methotrexate megadose followed by folate rescue. II. Clearance patterns in patients receiving sequential megadose infusions.", "content": "In some patients receiving megadose infusions of methotrexate (MTX), the rate of drug clearance decreases with each subsequent infusion. This can be modified by administration of fluids as outlined in the Recommended Protocol. Monitoring of each treatment is needed to assess requirements of fluids since these cannot be adequately predicted from data of previous infusions.", "contents": "Methotrexate megadose followed by folate rescue. II. Clearance patterns in patients receiving sequential megadose infusions. In some patients receiving megadose infusions of methotrexate (MTX), the rate of drug clearance decreases with each subsequent infusion. This can be modified by administration of fluids as outlined in the Recommended Protocol. Monitoring of each treatment is needed to assess requirements of fluids since these cannot be adequately predicted from data of previous infusions."}
{"id": "PMID:1085407", "title": "[Extracorporeal blood irradiation in chronic lymphatic leukemia (author's transl)].", "content": "In 8 outpatients suffering from different stages of chronic lymphatic leukemia extracorporeal irradiation of blood was used. In two cases full remission could be induced. In the other patients leukocytes were reduced to 10000 to 40000. In all patients we found a diminuation of B lymphocytes. Side effects were not observed.", "contents": "[Extracorporeal blood irradiation in chronic lymphatic leukemia (author's transl)]. In 8 outpatients suffering from different stages of chronic lymphatic leukemia extracorporeal irradiation of blood was used. In two cases full remission could be induced. In the other patients leukocytes were reduced to 10000 to 40000. In all patients we found a diminuation of B lymphocytes. Side effects were not observed."}
{"id": "PMID:1085410", "title": "Receptors for sheep erythrocytes (E) and erythrocyte-antibody-complement complexes (EAC) on the lymphoblasts of childhood acute lymphocyblastic leukemia.", "content": "Two cell-surface markers, rosette formation with sheep erythrocytes (E-rosette) as a T-cell marker and rosette formation with bovine erythrocyte-antibody-complement complex (EAC-rosette) as a B-cell marker were determined on peripheral blood lymphocytes and lymphoblasts from normal and 89 children with acute lymphoblastic leukemia (ALL). In the majority of the patients (12/15 untreated patients and 6/11 patients in relapse), lymphoblasts exhibited neither E- nor EAC-rosette formation. Lymphoblasts from one untreated patient with mediastinal mass displayed E-(50%) and EAC-rosette formation (15%). In 3 of 11 patients in relapse, lymphoblasts displayed an increase in EAC-rosette formation with progressive disease. In the remaining patients with active disease, a small and variable proportion of lymphoblasts expressed E and/or EAC-rosette formation. In 63 patients in remission, percentages of E- and/or EAC-rosette were similar (p greater than 0.05) to those of control. The results indicate a wide heterogeniety with respect to expression of lymphocyte membrane markers in lymphoblasts and in normal lymphocytes in patients with active ALL.", "contents": "Receptors for sheep erythrocytes (E) and erythrocyte-antibody-complement complexes (EAC) on the lymphoblasts of childhood acute lymphocyblastic leukemia. Two cell-surface markers, rosette formation with sheep erythrocytes (E-rosette) as a T-cell marker and rosette formation with bovine erythrocyte-antibody-complement complex (EAC-rosette) as a B-cell marker were determined on peripheral blood lymphocytes and lymphoblasts from normal and 89 children with acute lymphoblastic leukemia (ALL). In the majority of the patients (12/15 untreated patients and 6/11 patients in relapse), lymphoblasts exhibited neither E- nor EAC-rosette formation. Lymphoblasts from one untreated patient with mediastinal mass displayed E-(50%) and EAC-rosette formation (15%). In 3 of 11 patients in relapse, lymphoblasts displayed an increase in EAC-rosette formation with progressive disease. In the remaining patients with active disease, a small and variable proportion of lymphoblasts expressed E and/or EAC-rosette formation. In 63 patients in remission, percentages of E- and/or EAC-rosette were similar (p greater than 0.05) to those of control. The results indicate a wide heterogeniety with respect to expression of lymphocyte membrane markers in lymphoblasts and in normal lymphocytes in patients with active ALL."}
{"id": "PMID:1085411", "title": "Bacteriophage N3 of Haemophilus influenzae. I. Independence of vegetative recombination among Haemophilus influenzae bacteriophage on the host cell.", "content": "Vegtative recombination of temperature-sensitive mutants of Haemophilus influezae bacteriophage N3 was measured in wild-type H. influenzae strain Rd9 and in recombination-defective mutants of the Rd strain. Recombinants are formed with low but equal frequency in wild-type cells and recombination-defective mutants of the Rd strain. It is concluded that this phage can carry information in its own genome for vegetative recombination. Lysogenization readily takes place in both strains.", "contents": "Bacteriophage N3 of Haemophilus influenzae. I. Independence of vegetative recombination among Haemophilus influenzae bacteriophage on the host cell. Vegtative recombination of temperature-sensitive mutants of Haemophilus influezae bacteriophage N3 was measured in wild-type H. influenzae strain Rd9 and in recombination-defective mutants of the Rd strain. Recombinants are formed with low but equal frequency in wild-type cells and recombination-defective mutants of the Rd strain. It is concluded that this phage can carry information in its own genome for vegetative recombination. Lysogenization readily takes place in both strains."}
{"id": "PMID:1085412", "title": "Phospholipids bound to acidic nuclear proteins in human B and T lymphocytes.", "content": "Chromatin fractions (DNA, histones and non-histone chromosomal proteins NHCP) have been isolated from human peripheral B and T lymphocytes using different methods and analyzed in order to identify their lipid content. While DNA and histone fractions do not reveal the presence of lipids, a 2% of phospholipids is present in the NHCP fraction. The phospholipids associated with NHCP present a constant relative ratio among sphingomyelin, phosphatidyl-choline and phosphatidyl-ethanolamine both in B and T lymphocytes, whichever are the extraction procedures employed. These findings are related to the possible dereprssive role of phospholipids on DNA-dependent RNA synthesis.", "contents": "Phospholipids bound to acidic nuclear proteins in human B and T lymphocytes. Chromatin fractions (DNA, histones and non-histone chromosomal proteins NHCP) have been isolated from human peripheral B and T lymphocytes using different methods and analyzed in order to identify their lipid content. While DNA and histone fractions do not reveal the presence of lipids, a 2% of phospholipids is present in the NHCP fraction. The phospholipids associated with NHCP present a constant relative ratio among sphingomyelin, phosphatidyl-choline and phosphatidyl-ethanolamine both in B and T lymphocytes, whichever are the extraction procedures employed. These findings are related to the possible dereprssive role of phospholipids on DNA-dependent RNA synthesis."}
{"id": "PMID:1085424", "title": "The effect of electrical stimulation and drugs on the release of acetylcholine from the frog spinal cord.", "content": "The isolated frog spinal cord preparation, hemisected longitudinally, has been used to study concurrently the release of endogenous acetylcholine (ACh) and the electrical responses to stimulation of the ventral or dorsal roots. In unstimulated cords, ACh release was high initially and then fell to a plateau level. ACh release was reduced by tetrodotoxin and increased by high K+. Stimulation of the ventral roots enhanced the ACh release. The magnitude of this effect was dependent on the frequency of stimulation and was antagonized by tetrodotoxin or C Ca2+-free Ringer. During prolonged ventral root stimulation (50 min), the evoked ACh release became progressively smaller but was restored to the original high levels by the addition of choline to the bath fluid. Stimulation of the dorsal roots failed to augment the ACh output. The application of hyoscine was followed by a large rise in the unstimulated ACh output. However no increase in the antidromically-evoked release, when compared with control stimulations, was seen. The application on alpha-bungarotoxin was followed by a short-lasting decline in the unstimulated ACh output, whereas the electrically-evoked output was unaffected. In the frog spinal cord the determination of ACh release, together with the recording of the root potentials, offers the possibility of studying the output of a central transmitter in relation to the electrical activity of an in vitro central nervous system preparation.", "contents": "The effect of electrical stimulation and drugs on the release of acetylcholine from the frog spinal cord. The isolated frog spinal cord preparation, hemisected longitudinally, has been used to study concurrently the release of endogenous acetylcholine (ACh) and the electrical responses to stimulation of the ventral or dorsal roots. In unstimulated cords, ACh release was high initially and then fell to a plateau level. ACh release was reduced by tetrodotoxin and increased by high K+. Stimulation of the ventral roots enhanced the ACh release. The magnitude of this effect was dependent on the frequency of stimulation and was antagonized by tetrodotoxin or C Ca2+-free Ringer. During prolonged ventral root stimulation (50 min), the evoked ACh release became progressively smaller but was restored to the original high levels by the addition of choline to the bath fluid. Stimulation of the dorsal roots failed to augment the ACh output. The application of hyoscine was followed by a large rise in the unstimulated ACh output. However no increase in the antidromically-evoked release, when compared with control stimulations, was seen. The application on alpha-bungarotoxin was followed by a short-lasting decline in the unstimulated ACh output, whereas the electrically-evoked output was unaffected. In the frog spinal cord the determination of ACh release, together with the recording of the root potentials, offers the possibility of studying the output of a central transmitter in relation to the electrical activity of an in vitro central nervous system preparation."}
{"id": "PMID:1085428", "title": "[A clinicopathological study of the cranial \"histiocytosis X\" (AUTHOR'S TRANSL)].", "content": "A clinicopathological study of 13 cases of cranial histiocytosis X was discussed. The patients were mostly infants, and those under 15 years of age numbered eight. Hematological, blood chemistrical, and other laboratorical prodedures have produced no direct diagnostic value. Radiographically, this lesion in skull appeared to be a lytic defect in all cases. Histologically, they were unified by a localized or generalized histiocytic proliferation. Eosinophilic cells were also found throughout the lesional tissue. There were some slight histological variations from case to case, for instance, in the degree of infiltration by eosinophilic cells, and the number of the histiocytic cells. However, the basic histological pattern remained the same in all cases. The lesion may already have extended into the neighboring osseous tissues. Sections of the neighboring osseous tissues showed the intertrabecular spaces to be infiltrated by the same type of lesion. The patient related trauma to the onset of symptoms in 6 cases, but it is true that we know nothing of the etiology of this lesion. The treatment of choice is the adequate surgical extirpation. It seems fair to conclude that wide surgical excision with postoperative irradiation or with chemotherapy may be expected to affect a clinical cure.", "contents": "[A clinicopathological study of the cranial \"histiocytosis X\" (AUTHOR'S TRANSL)]. A clinicopathological study of 13 cases of cranial histiocytosis X was discussed. The patients were mostly infants, and those under 15 years of age numbered eight. Hematological, blood chemistrical, and other laboratorical prodedures have produced no direct diagnostic value. Radiographically, this lesion in skull appeared to be a lytic defect in all cases. Histologically, they were unified by a localized or generalized histiocytic proliferation. Eosinophilic cells were also found throughout the lesional tissue. There were some slight histological variations from case to case, for instance, in the degree of infiltration by eosinophilic cells, and the number of the histiocytic cells. However, the basic histological pattern remained the same in all cases. The lesion may already have extended into the neighboring osseous tissues. Sections of the neighboring osseous tissues showed the intertrabecular spaces to be infiltrated by the same type of lesion. The patient related trauma to the onset of symptoms in 6 cases, but it is true that we know nothing of the etiology of this lesion. The treatment of choice is the adequate surgical extirpation. It seems fair to conclude that wide surgical excision with postoperative irradiation or with chemotherapy may be expected to affect a clinical cure."}
{"id": "PMID:1085429", "title": "The medial posterior choroidal artery as an indicator of masses at the foramen of Monro.", "content": "The angiographic findings in five cases of tumors at the foramen of Monro are presented. Characteristic changes in the course and configuration of the medial posterior choroidal artery are described.", "contents": "The medial posterior choroidal artery as an indicator of masses at the foramen of Monro. The angiographic findings in five cases of tumors at the foramen of Monro are presented. Characteristic changes in the course and configuration of the medial posterior choroidal artery are described."}
{"id": "PMID:1085430", "title": "B and T lymphocytes in man. III. B, t, and \"null\" lymphocytes in multiple sclerosis.", "content": "T, B, and \"null\" lymphocytes were determined in peripheral blood obtained from 24 control subjects and 15 multiple sclerosis patients. The total number of lymphocytes and T lymphocytes were reduced significantly in multiple sclerosis patients as compared with those of controls. Although the percentage of B lymphocytes increased in these patients, the absolute number of B lymphocytes was similar to controls. The mitotic response of lymphocytes from multiple sclerosis patients to phytohemagglutinin and pokeweed mitogen in 7-day-old cultures was decreased as compared with that of controls.", "contents": "B and T lymphocytes in man. III. B, t, and \"null\" lymphocytes in multiple sclerosis. T, B, and \"null\" lymphocytes were determined in peripheral blood obtained from 24 control subjects and 15 multiple sclerosis patients. The total number of lymphocytes and T lymphocytes were reduced significantly in multiple sclerosis patients as compared with those of controls. Although the percentage of B lymphocytes increased in these patients, the absolute number of B lymphocytes was similar to controls. The mitotic response of lymphocytes from multiple sclerosis patients to phytohemagglutinin and pokeweed mitogen in 7-day-old cultures was decreased as compared with that of controls."}
{"id": "PMID:1085432", "title": "Malignant lymphoma and rheumatic symptoms.", "content": "A patient intially thought to have a connective tissue disease, later shown by biopsy to have Hodgkin's disease is described. The progress of her illness suggests that she is suffering from a connective tissue disease, too, or possibly secondary immune complex disease.", "contents": "Malignant lymphoma and rheumatic symptoms. A patient intially thought to have a connective tissue disease, later shown by biopsy to have Hodgkin's disease is described. The progress of her illness suggests that she is suffering from a connective tissue disease, too, or possibly secondary immune complex disease."}
{"id": "PMID:1085434", "title": "Mandibular histiocytosis X and acute lymphoblastic leukemia.", "content": "The occurrence together of two major distinct diseases in the oral area is relatively rare. In this report, a case is presented in which histiocytosis X was found to develop in a child with acute lymphoblastic leukemia. The nature of the differentiated histiocytoses is explored, and the possibility of the relationship between a lymphoproliferative and a histiocytic disease entity is investigated.", "contents": "Mandibular histiocytosis X and acute lymphoblastic leukemia. The occurrence together of two major distinct diseases in the oral area is relatively rare. In this report, a case is presented in which histiocytosis X was found to develop in a child with acute lymphoblastic leukemia. The nature of the differentiated histiocytoses is explored, and the possibility of the relationship between a lymphoproliferative and a histiocytic disease entity is investigated."}
{"id": "PMID:1085435", "title": "Evaluation of a rapid beta-lactamase test for detecting ampicillin-resistant strains of Hemophilus influenzae type b.", "content": "Chloramphenicol is presently the drug of choice in the initial treatment of serious infections due to Hemophilus influenzae type b. Rapid detection of ampicillin resistance in clinical isolates would facilitate early discontinuation of chloramphenicol therapy in patients infected with ampicillin-sensitive bacteria. A total of 160 strains of H. influenzae type b were tested with a one-hour acidimetric microassay for beta-lactamase activity. All ampicillin-resistant strains rapidly hydrolysed the beta-lactam ring of penicillin. When isolates were encoded and tested without knowledge of their MICs, the 40 ampicillin-resistant strains (MIC greater than or equal to 2 mug/ml) were readily distinguished from 120 sensitive strains. Rapid beta-lactamase assay is therefore a reliable detector of ampicillin resistance in H. influenzae type b.", "contents": "Evaluation of a rapid beta-lactamase test for detecting ampicillin-resistant strains of Hemophilus influenzae type b. Chloramphenicol is presently the drug of choice in the initial treatment of serious infections due to Hemophilus influenzae type b. Rapid detection of ampicillin resistance in clinical isolates would facilitate early discontinuation of chloramphenicol therapy in patients infected with ampicillin-sensitive bacteria. A total of 160 strains of H. influenzae type b were tested with a one-hour acidimetric microassay for beta-lactamase activity. All ampicillin-resistant strains rapidly hydrolysed the beta-lactam ring of penicillin. When isolates were encoded and tested without knowledge of their MICs, the 40 ampicillin-resistant strains (MIC greater than or equal to 2 mug/ml) were readily distinguished from 120 sensitive strains. Rapid beta-lactamase assay is therefore a reliable detector of ampicillin resistance in H. influenzae type b."}
{"id": "PMID:1085436", "title": "Epidemiologic characteristics of infections caused by ampicillin-resistant Hemophilus influenzae.", "content": "Hrom February 1974 through May 1975, 58 isolates of ampicillin-resistant Hemophilus influenzae from patients were submitted to the Center for Disease Control from 23 states and the District of Columbia. The epidemiologic characteristics of these widely scattered cases were strikingly similar to those of cases previously reported from individual communities or institutions. Because of the nationwide distribution of resistant isolates and the adverse consequences of ineffective therapy, the use of chloramphenicol is recommended for the initial management of systemic illness definitely or probably due to H. influenzae type b.", "contents": "Epidemiologic characteristics of infections caused by ampicillin-resistant Hemophilus influenzae. Hrom February 1974 through May 1975, 58 isolates of ampicillin-resistant Hemophilus influenzae from patients were submitted to the Center for Disease Control from 23 states and the District of Columbia. The epidemiologic characteristics of these widely scattered cases were strikingly similar to those of cases previously reported from individual communities or institutions. Because of the nationwide distribution of resistant isolates and the adverse consequences of ineffective therapy, the use of chloramphenicol is recommended for the initial management of systemic illness definitely or probably due to H. influenzae type b."}
{"id": "PMID:1085437", "title": "Asymmetrical displacement current and its relation with the activation of sodium current in the membrane of frog myelinated nerve.", "content": "1. Sodium currents (INa) and asymmetrical displacement currents (ID) were measured in the same nerve fibres from Rana esculenta under similar conditions. 2. For exploring possible kinetic and steady state relations between INa and ID the following quantities were compared: (i) the activation of the sodium channels and (ii) the charge displacement of ID. 3. The delay of sodium activation increased after hyperpolarization. A corresponding effect on the displacement of charge was not observed. 4. Upon a small depolarization sodium activation rose slower than the displacement of charge, whereas at large depolarizations sodium activation reached a steady level before the charge displacement. 5. Upon repolarization to various levels between -52 and 12 mV relative to the resting potential, the ratio between the time constants of charge displacement and of sodium tail current varied between 3 and 1. 6. In the steady state the sodium activation was one half at about the same potential as the charge displacement but exhibited a clearly steeper voltage dependence. 7. Blocage of sodium channels with tetrodotoxin did not affect the asymmetrical displacement current. Replacing a part of external Na by tris did not alter the sodijm activation process. 8. The results indicate that the asymmetrical displacement of charge may reflect states of the gating mechanism in sodium channels but cannot be considered as a correlate of the Hodgkin Huxley m variable.", "contents": "Asymmetrical displacement current and its relation with the activation of sodium current in the membrane of frog myelinated nerve. 1. Sodium currents (INa) and asymmetrical displacement currents (ID) were measured in the same nerve fibres from Rana esculenta under similar conditions. 2. For exploring possible kinetic and steady state relations between INa and ID the following quantities were compared: (i) the activation of the sodium channels and (ii) the charge displacement of ID. 3. The delay of sodium activation increased after hyperpolarization. A corresponding effect on the displacement of charge was not observed. 4. Upon a small depolarization sodium activation rose slower than the displacement of charge, whereas at large depolarizations sodium activation reached a steady level before the charge displacement. 5. Upon repolarization to various levels between -52 and 12 mV relative to the resting potential, the ratio between the time constants of charge displacement and of sodium tail current varied between 3 and 1. 6. In the steady state the sodium activation was one half at about the same potential as the charge displacement but exhibited a clearly steeper voltage dependence. 7. Blocage of sodium channels with tetrodotoxin did not affect the asymmetrical displacement current. Replacing a part of external Na by tris did not alter the sodijm activation process. 8. The results indicate that the asymmetrical displacement of charge may reflect states of the gating mechanism in sodium channels but cannot be considered as a correlate of the Hodgkin Huxley m variable."}
{"id": "PMID:1085438", "title": "Saxitoxin and procaine act independently on separate sites of the sodium channel.", "content": "1. Voltage clamp experiments were done on single myelinated nerve fibres of the frog, Rana esculenta. 2. The time course of procaine action (1.0 mM at pH 7.2) was obtained from changes in INa on changing solutions during repetitive (1 HZ) depolarizing pulses of constant amplitude following hyperpolarizing prepulses. The mean half times of onset and offset of procaine block were 3.7 and 28 s, respectively. In the presence of 1.4 nM saxitoxin (STX) the corresponding times were virtually the same, 3.1 and 27 s. 3. Similarly, the time course of partial relief from procaine block that is obtained by increasing the frequency of the prepulse-test pulse pairs from 1-10 HZ was unaffected in the presence of STX. 4. Comparison of the equilibrium effects of procaine concentrations ranging from 0.03-1.0 mM suggest a one-to-one drug-receptor reaction. The fraction of Na channels blocked at equilibrium with 1.0 mM procaine, 1.4 nM STX, and 1.0 mM procaine + 1.4 nM STX was 0.81, 0.49, and 0.90, respectively. This result and the kinetic behaviour fully agree with the idea of two separate and independent receptors for procaine and STX.", "contents": "Saxitoxin and procaine act independently on separate sites of the sodium channel. 1. Voltage clamp experiments were done on single myelinated nerve fibres of the frog, Rana esculenta. 2. The time course of procaine action (1.0 mM at pH 7.2) was obtained from changes in INa on changing solutions during repetitive (1 HZ) depolarizing pulses of constant amplitude following hyperpolarizing prepulses. The mean half times of onset and offset of procaine block were 3.7 and 28 s, respectively. In the presence of 1.4 nM saxitoxin (STX) the corresponding times were virtually the same, 3.1 and 27 s. 3. Similarly, the time course of partial relief from procaine block that is obtained by increasing the frequency of the prepulse-test pulse pairs from 1-10 HZ was unaffected in the presence of STX. 4. Comparison of the equilibrium effects of procaine concentrations ranging from 0.03-1.0 mM suggest a one-to-one drug-receptor reaction. The fraction of Na channels blocked at equilibrium with 1.0 mM procaine, 1.4 nM STX, and 1.0 mM procaine + 1.4 nM STX was 0.81, 0.49, and 0.90, respectively. This result and the kinetic behaviour fully agree with the idea of two separate and independent receptors for procaine and STX."}
{"id": "PMID:1085439", "title": "Studies on the relation between latency relaxation and resting cross-bridges of frog skeletal muscle.", "content": "Latency relaxation of frog skeletal muscle (LR) was investigated with respect to its relation to resting cross-bridges. A decrease in the initial stiffness of the resting muscle (stiffness of the muscle during the beginning of a length-change) was found, when repeated triangular length-changes were imposed on the muscle. This decrease in the initial stiffness depends on the velocity of the length-change. It is interpreted that the decrease in the initial stiffness reflects a detachment of the resting cross-bridges from their binding-sites. The LR, induced immediately after the offset of the length-changes, i.e. when the cross-bridges are still detached, showed an increased depth, its time course remaining unchanged. There is a strong correlation between the increase in the depth of the LR and the decrease in the initial stiffness. The LR regained its original depth (depth without a preceding length-change) about 5 s after the offset of the length-changes (20 degrees C). It is suggested that the LR of skeletal muscle is not due to a detachment of resting cross-bridges.", "contents": "Studies on the relation between latency relaxation and resting cross-bridges of frog skeletal muscle. Latency relaxation of frog skeletal muscle (LR) was investigated with respect to its relation to resting cross-bridges. A decrease in the initial stiffness of the resting muscle (stiffness of the muscle during the beginning of a length-change) was found, when repeated triangular length-changes were imposed on the muscle. This decrease in the initial stiffness depends on the velocity of the length-change. It is interpreted that the decrease in the initial stiffness reflects a detachment of the resting cross-bridges from their binding-sites. The LR, induced immediately after the offset of the length-changes, i.e. when the cross-bridges are still detached, showed an increased depth, its time course remaining unchanged. There is a strong correlation between the increase in the depth of the LR and the decrease in the initial stiffness. The LR regained its original depth (depth without a preceding length-change) about 5 s after the offset of the length-changes (20 degrees C). It is suggested that the LR of skeletal muscle is not due to a detachment of resting cross-bridges."}
{"id": "PMID:1085440", "title": "Renal test dyes III. Effect of dyes suitable for renal passage time measurements on renal function.", "content": "The effects of the triaryl-methane dyes Lissamine Green SF (LF SF). Lissamine Green BN (LG BN), Lissamine Green V (LG V) and Kiton Pure Blue V (KPB V) on renal functions of rats and on sodium transport across isolated frog skin have been investigated. The experiments failed to show any natriuretic or diuretic effects of the purified dyes on the rat kidney. In the continuous infusion experiments, which lasted 3 1/2 h, the amount of the dye infused was equivalent to a passage time measurement every 3 min. In rapid injection experiments LG V was injected every 5 min for 1 h. The dyes did not have any effect on insulin clearance either. Previously reported effects of the dyes can be partly explained by the presence of sodium sulfate in commercially available dyes. Experiments with LG V showed that the dye also has no effect on the potential difference or short circuit current across the isolated frog skin. This supports their usefulness as renal test dyes.", "contents": "Renal test dyes III. Effect of dyes suitable for renal passage time measurements on renal function. The effects of the triaryl-methane dyes Lissamine Green SF (LF SF). Lissamine Green BN (LG BN), Lissamine Green V (LG V) and Kiton Pure Blue V (KPB V) on renal functions of rats and on sodium transport across isolated frog skin have been investigated. The experiments failed to show any natriuretic or diuretic effects of the purified dyes on the rat kidney. In the continuous infusion experiments, which lasted 3 1/2 h, the amount of the dye infused was equivalent to a passage time measurement every 3 min. In rapid injection experiments LG V was injected every 5 min for 1 h. The dyes did not have any effect on insulin clearance either. Previously reported effects of the dyes can be partly explained by the presence of sodium sulfate in commercially available dyes. Experiments with LG V showed that the dye also has no effect on the potential difference or short circuit current across the isolated frog skin. This supports their usefulness as renal test dyes."}
{"id": "PMID:1085449", "title": "Aplastic anemia: presence in human bone marrow of cells that suppress myelopoiesis.", "content": "Bone marrow from a patient with aplastic anemia was shown by multiple criteria to have a block in early myeloid differentiation. This block was overcome in vitro by elimination of marrow lymphocytes. Furthermore, this differentiation block was transferred in vitro to normal marrow by coculturing with the patient's marrow. We suggest that some cases of aplastic anemia may be due to an immunologically based suppression of marrow cell differentiation rather than to a defect in stem cells or their necessary inductive environment.", "contents": "Aplastic anemia: presence in human bone marrow of cells that suppress myelopoiesis. Bone marrow from a patient with aplastic anemia was shown by multiple criteria to have a block in early myeloid differentiation. This block was overcome in vitro by elimination of marrow lymphocytes. Furthermore, this differentiation block was transferred in vitro to normal marrow by coculturing with the patient's marrow. We suggest that some cases of aplastic anemia may be due to an immunologically based suppression of marrow cell differentiation rather than to a defect in stem cells or their necessary inductive environment."}
{"id": "PMID:1085450", "title": "Von Willebrand's disease--a potential bleeding problem in plastic surgery.", "content": "Von Willebrand's disease, a common hereditary bleeding disorder, may be responsible for undue or unanticipated intraoperative or postoperative hemorrhage. The diagnosis may be difficult to establish, although it may be suspected clinically and can usually be confirmed by adequate laboratory investigation. Patients with Von Willebrand's disease appear to be especially susceptible to developing significant prolongation of their bleeding time after the ingestion of aspirin.", "contents": "Von Willebrand's disease--a potential bleeding problem in plastic surgery. Von Willebrand's disease, a common hereditary bleeding disorder, may be responsible for undue or unanticipated intraoperative or postoperative hemorrhage. The diagnosis may be difficult to establish, although it may be suspected clinically and can usually be confirmed by adequate laboratory investigation. Patients with Von Willebrand's disease appear to be especially susceptible to developing significant prolongation of their bleeding time after the ingestion of aspirin."}
{"id": "PMID:1085452", "title": "Effects of tandamine and pirandamine, new potential antidepressants, on the brain uptake of norepinephrine and 5-hydroxytryptamine and related activities.", "content": "Two novel agents, tandamine (TA; a thiopyrano (3,4-b) indole) and pirandamine (PA; an indeno (2,1-c)pyran), and the tricyclic antidepressants desimipramine (DMI), imipramine (I) and amitriptyline (A) were compared in various in vivo pharmacological tests and for norepinephrine (NE) and 5-hydroxytryptamine (5-HT) neuronal uptake inhibition. TA was found to be equivalent, or greater, in activity to DMI in blocking brain NE uptake, antagonizing reserpine-induced effects and potentiating the behavioural effects of l-Dopa. Similarly to DMI, TA did not appreciably block brain 5-HT uptake; unlike DMI, TA did potentiate central 5-HT activity at high doses. PA exerted an opposite profile to TA, being equivalent to A and greater than I as a 5-HT uptake blocker and central 5-HT potentiator; PA was not effective as a NE uptake blocker or potentiator. Neither TA or PA exhibited in vivo MAO inhibition, and in contrast to DMI, I and A, exhibited no central anticholinergic effects. TA, but not PA, potentiated apomorphine-induced gnawing. These findings indicate that TA is a relatively specific blocker of neuronal NE uptake and PA is a selective 5-HT uptake blocker.", "contents": "Effects of tandamine and pirandamine, new potential antidepressants, on the brain uptake of norepinephrine and 5-hydroxytryptamine and related activities. Two novel agents, tandamine (TA; a thiopyrano (3,4-b) indole) and pirandamine (PA; an indeno (2,1-c)pyran), and the tricyclic antidepressants desimipramine (DMI), imipramine (I) and amitriptyline (A) were compared in various in vivo pharmacological tests and for norepinephrine (NE) and 5-hydroxytryptamine (5-HT) neuronal uptake inhibition. TA was found to be equivalent, or greater, in activity to DMI in blocking brain NE uptake, antagonizing reserpine-induced effects and potentiating the behavioural effects of l-Dopa. Similarly to DMI, TA did not appreciably block brain 5-HT uptake; unlike DMI, TA did potentiate central 5-HT activity at high doses. PA exerted an opposite profile to TA, being equivalent to A and greater than I as a 5-HT uptake blocker and central 5-HT potentiator; PA was not effective as a NE uptake blocker or potentiator. Neither TA or PA exhibited in vivo MAO inhibition, and in contrast to DMI, I and A, exhibited no central anticholinergic effects. TA, but not PA, potentiated apomorphine-induced gnawing. These findings indicate that TA is a relatively specific blocker of neuronal NE uptake and PA is a selective 5-HT uptake blocker."}
{"id": "PMID:1085455", "title": "The usefulness of computed tomography during and after radiation therapy in patients with brain tumors.", "content": "Two years of experience with 100 patients in the serial study of brain tumors during and after radiation therapy has shown that computed tomography is useful in (a) depiction of regression or growth of primary and metastatic intracranial tumors, (b) recognition of untoward sequelae of radiation therapy, such as necrosis and edema, and (c) appreciation of changes in tumor density and ventricular size. Representation cases are discussed and illustrated.", "contents": "The usefulness of computed tomography during and after radiation therapy in patients with brain tumors. Two years of experience with 100 patients in the serial study of brain tumors during and after radiation therapy has shown that computed tomography is useful in (a) depiction of regression or growth of primary and metastatic intracranial tumors, (b) recognition of untoward sequelae of radiation therapy, such as necrosis and edema, and (c) appreciation of changes in tumor density and ventricular size. Representation cases are discussed and illustrated."}
{"id": "PMID:1085456", "title": "Computed tomography in the evaluation of malignant glioma before and after therapy.", "content": "Sixty-three patients with malignant glioma undergoing chemotherapy and radiotherapy were evaluated with electroencephalography, 99mTc-DTPA imaging, and computed tomography (CT). Tumor size, central lucency, contrast enhancement, surrounding edema, and ventricular size were assessed. CT findings were found to be reliable and often predicted the clinical course. Tumor size, central lucency, and contrast enhancement increased in patients with clinical deterioration and decreased in those with improvement. The CT scan also provided additional information of value in adjunctive therapy, such as the presence of cysts or ventricular obstruction. In general, CT gave a more complete profile of tumor characteristics than other diagnostic modalities.", "contents": "Computed tomography in the evaluation of malignant glioma before and after therapy. Sixty-three patients with malignant glioma undergoing chemotherapy and radiotherapy were evaluated with electroencephalography, 99mTc-DTPA imaging, and computed tomography (CT). Tumor size, central lucency, contrast enhancement, surrounding edema, and ventricular size were assessed. CT findings were found to be reliable and often predicted the clinical course. Tumor size, central lucency, and contrast enhancement increased in patients with clinical deterioration and decreased in those with improvement. The CT scan also provided additional information of value in adjunctive therapy, such as the presence of cysts or ventricular obstruction. In general, CT gave a more complete profile of tumor characteristics than other diagnostic modalities."}
{"id": "PMID:1085478", "title": "Air pollution in copenhagen. Part I. Element analysis and size distribution of aerosols.", "content": "Aerosols were monitored in Greater Copenhagen in the period June 1973 to July 1974. Size-fractionated cascade impactor samples and unfractionated filter samples were regularly collected and analyzed by neutron activation analysis, spark emission spectroscopy or proton-induced X-ray emission spectroscopy. Concentrations were determined of the following elements: Al, Si, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, Br, Mo, Cd, Sn, Sb, and Pb. All elements showed orders-of-magnitude fluctuations; the mean concentrations were roughly the same as in other large cities. In relation to proposed air quality standards, Pb was the most critical component. Statistical analysis of variation patterns, size distributions and interelement correlations indicate that automotive exhaust is the source of Br and Pb; fuel-oil combustion is the main source of V and Ni (and partly of S); soil dust raised by wind or by human activity (e. g. traffic) is the main source of Al, Si, Ca, Ti, and Fe.", "contents": "Air pollution in copenhagen. Part I. Element analysis and size distribution of aerosols. Aerosols were monitored in Greater Copenhagen in the period June 1973 to July 1974. Size-fractionated cascade impactor samples and unfractionated filter samples were regularly collected and analyzed by neutron activation analysis, spark emission spectroscopy or proton-induced X-ray emission spectroscopy. Concentrations were determined of the following elements: Al, Si, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, Br, Mo, Cd, Sn, Sb, and Pb. All elements showed orders-of-magnitude fluctuations; the mean concentrations were roughly the same as in other large cities. In relation to proposed air quality standards, Pb was the most critical component. Statistical analysis of variation patterns, size distributions and interelement correlations indicate that automotive exhaust is the source of Br and Pb; fuel-oil combustion is the main source of V and Ni (and partly of S); soil dust raised by wind or by human activity (e. g. traffic) is the main source of Al, Si, Ca, Ti, and Fe."}
{"id": "PMID:1085482", "title": "[Angiographic diagnosis of localized gastro-Intestinal hemorrhage (author's transl)].", "content": "Origin and site of gastro-intestinal hemorrhage in 3 cases of acute and 14 cases of chronic bleeding are described. In such cases of angiodysplasia other investigations and, in some, even repeated operation could not find the origin, but angiography succeeded.", "contents": "[Angiographic diagnosis of localized gastro-Intestinal hemorrhage (author's transl)]. Origin and site of gastro-intestinal hemorrhage in 3 cases of acute and 14 cases of chronic bleeding are described. In such cases of angiodysplasia other investigations and, in some, even repeated operation could not find the origin, but angiography succeeded."}
{"id": "PMID:1085484", "title": "Modification of the response of human T lymphocytes to phytomitogens by cocultivation with unresponsive non-T leukocytes.", "content": "Experiments were conducted to ascertain to what extent the mitogen response of human peripheral T cells is influenced by contaminated, nonresponding leukocytes. T- and B-lymphocyte preparations, extensively depleted of monocytes, exhibited extremely poor responses to pokeweed mitogen and concanavalin A compared with the original unfractionated cell population. A strongly reduced phytohemagglutinin response was noted in the B-cell but not in the T-cell fraction. Mixtures of cell preparations enriched in T or B cells yielded stimulations higher than expected. Mitomycin-C-treated T-cell preparations mixed with untreated preparations enriched in B cells gave slightly higher responses than expected. However, highly enhanced responses were observed when untreated T cells were mixed with mitomycin-treated cell suspensions enriched in B cells. A similar enhancing effect was noted when mitomycin-treated cell suspensions enriched in adherent cells were added. It is concluded that the mitogen reactivity of T cells, on a cell-for-cell basis, may be enhanced tenfold or more by cocultivation with unresponsive non-T leukocytes. A reduced mitogen reactivity of peripheral lymphoid cells from patients with various types of disease may thus either reflect a decreased proportion of responsive lymphocytes or unresponsive non-T lymphocytes capable of enhancing mitogen reactivity, or both.", "contents": "Modification of the response of human T lymphocytes to phytomitogens by cocultivation with unresponsive non-T leukocytes. Experiments were conducted to ascertain to what extent the mitogen response of human peripheral T cells is influenced by contaminated, nonresponding leukocytes. T- and B-lymphocyte preparations, extensively depleted of monocytes, exhibited extremely poor responses to pokeweed mitogen and concanavalin A compared with the original unfractionated cell population. A strongly reduced phytohemagglutinin response was noted in the B-cell but not in the T-cell fraction. Mixtures of cell preparations enriched in T or B cells yielded stimulations higher than expected. Mitomycin-C-treated T-cell preparations mixed with untreated preparations enriched in B cells gave slightly higher responses than expected. However, highly enhanced responses were observed when untreated T cells were mixed with mitomycin-treated cell suspensions enriched in B cells. A similar enhancing effect was noted when mitomycin-treated cell suspensions enriched in adherent cells were added. It is concluded that the mitogen reactivity of T cells, on a cell-for-cell basis, may be enhanced tenfold or more by cocultivation with unresponsive non-T leukocytes. A reduced mitogen reactivity of peripheral lymphoid cells from patients with various types of disease may thus either reflect a decreased proportion of responsive lymphocytes or unresponsive non-T lymphocytes capable of enhancing mitogen reactivity, or both."}
{"id": "PMID:1085479", "title": "[Partial syndrome of cerebello pontine region due to an aneurysm of the internal auditory artery (author's transl)].", "content": "An aneurysm of the distal portion of the middle cerebellar artery was first clipped and then, given the type of neck, removed. The case is reported because of its extreme rarity, the unusual succession of symptoms, the difficulty of diagnosing the malformation, (thanks to modern radiological techniques) and the successful outcome of surgery.", "contents": "[Partial syndrome of cerebello pontine region due to an aneurysm of the internal auditory artery (author's transl)]. An aneurysm of the distal portion of the middle cerebellar artery was first clipped and then, given the type of neck, removed. The case is reported because of its extreme rarity, the unusual succession of symptoms, the difficulty of diagnosing the malformation, (thanks to modern radiological techniques) and the successful outcome of surgery."}
{"id": "PMID:1085485", "title": "Binding of IgM by human T lymphocytes.", "content": "Ox erythrocytes, sensitized with IgM-type rabbit antibodies, were found to form rosettes with human blood lymphocytes. Granulocytes, monocytes, and macrophages appeared to lack this property. The rosette formation could be inhibited with human IgM and its Fc fragments. IgM-binding lymphocytes were also present in cell suspensions isolated from human tonsils and thymuses and among blood lymphocytes cultured in vitro. The outcome of combined marker tests and cell separation experiments disclosed that the rosetting lymphocytes belong to the T-lymphocyte population.", "contents": "Binding of IgM by human T lymphocytes. Ox erythrocytes, sensitized with IgM-type rabbit antibodies, were found to form rosettes with human blood lymphocytes. Granulocytes, monocytes, and macrophages appeared to lack this property. The rosette formation could be inhibited with human IgM and its Fc fragments. IgM-binding lymphocytes were also present in cell suspensions isolated from human tonsils and thymuses and among blood lymphocytes cultured in vitro. The outcome of combined marker tests and cell separation experiments disclosed that the rosetting lymphocytes belong to the T-lymphocyte population."}
{"id": "PMID:1085486", "title": "Characterization of dextran-sulfate-sensitive cells.", "content": "The cell type participating in the mitotic response to the polyclonal B-cell activator (PBA) dextran-sulfate (DS) was investigated. Cells from murine fetal liver, adult bone marrow, and spleen were studied; only a limited number of all cells present in each organ responded to DS. Morphological studies of the activated cells showed the major population of activated cells in spleen to have the appearance of lymphoblasts. In bone marrow, several classes of hematopoietic cells were mitotically active, including mononuclear cells (lymphoblasts and monocytes), megakaryocytes, and myeloblasts. In these cultures, however, it was not possible to differentiate between DS-activated and spontaneously proliferating cells. Bone marrow and, to some extent, spleen cell cultures activated with DS contained a relative increase in numbers of phagocytic cells, whereas stimulation of spleen cells with lipopolysaccharide did not result in an increased phagocytosis. However, adherent cells were not necessary for activation of DNA synthesis by DS in spleen, and this cell type did not contribute to a measurable degree to the DNA synthetical response. DS cannot be regarded as a general stem cell mitogen for bone marrow cells since it failed to promote colony growth of hematopoietic cells in an in vitro system. However, supernatants from DS-activated spleen and bone marrow cell cultures did stimulate colony growth of murine bone marrow cells, indicating that stem cells of nonlymphoid origin might be indirectly activated by DS. In conclusion, the major cell population activated by DS in spleen is lymphocytes. In bone marrow, other cell types seem to participate in the response as well, but the activation mechanism may be indirect and not primarily the result of DS interaction with these cells.", "contents": "Characterization of dextran-sulfate-sensitive cells. The cell type participating in the mitotic response to the polyclonal B-cell activator (PBA) dextran-sulfate (DS) was investigated. Cells from murine fetal liver, adult bone marrow, and spleen were studied; only a limited number of all cells present in each organ responded to DS. Morphological studies of the activated cells showed the major population of activated cells in spleen to have the appearance of lymphoblasts. In bone marrow, several classes of hematopoietic cells were mitotically active, including mononuclear cells (lymphoblasts and monocytes), megakaryocytes, and myeloblasts. In these cultures, however, it was not possible to differentiate between DS-activated and spontaneously proliferating cells. Bone marrow and, to some extent, spleen cell cultures activated with DS contained a relative increase in numbers of phagocytic cells, whereas stimulation of spleen cells with lipopolysaccharide did not result in an increased phagocytosis. However, adherent cells were not necessary for activation of DNA synthesis by DS in spleen, and this cell type did not contribute to a measurable degree to the DNA synthetical response. DS cannot be regarded as a general stem cell mitogen for bone marrow cells since it failed to promote colony growth of hematopoietic cells in an in vitro system. However, supernatants from DS-activated spleen and bone marrow cell cultures did stimulate colony growth of murine bone marrow cells, indicating that stem cells of nonlymphoid origin might be indirectly activated by DS. In conclusion, the major cell population activated by DS in spleen is lymphocytes. In bone marrow, other cell types seem to participate in the response as well, but the activation mechanism may be indirect and not primarily the result of DS interaction with these cells."}
{"id": "PMID:1085487", "title": "Off-signals in lymphocyte activation.", "content": "High-dose unresponsiveness to concanavalin A affected T and B lymphocytes equally, although only T cells were activated by Con A. Thus, activation and inactivation signals are totally unrelated, the latter also lacking target cell specificity. The off-signal caused cell death and is probably biologically unimportant.", "contents": "Off-signals in lymphocyte activation. High-dose unresponsiveness to concanavalin A affected T and B lymphocytes equally, although only T cells were activated by Con A. Thus, activation and inactivation signals are totally unrelated, the latter also lacking target cell specificity. The off-signal caused cell death and is probably biologically unimportant."}
{"id": "PMID:1085488", "title": "Specific and nonspecific cooperation between M-locus-incompatible T and B cells during adoptive anti-hapten antibody responses in mice.", "content": "CBA and AKR mice are identical at the major histocompatibility locus (H-2) but differ at the strong, non-H-2, mixed leukocyte culture (MLC)-stimulating M-locus. Adoptive secondary in vivo antibody responses using immune spleen cells from mice of these strains demonstrate two interesting findings. One is a non-specific augmentation of the anti-hapten antibody response of CBA B cells by AKR T cells. This allogeneic effect is unidirectional in the same sense as the MLC between cells from these two strains. The second is that, despite an ongoing allogeneic effect, M-locus-incompatible T and B cells show strong specific cooperation. Thus, it seems unlikely that the failure of H-2-incompatible T and B cells to collaborate specifically is due to inhibitory allogenic reactions.", "contents": "Specific and nonspecific cooperation between M-locus-incompatible T and B cells during adoptive anti-hapten antibody responses in mice. CBA and AKR mice are identical at the major histocompatibility locus (H-2) but differ at the strong, non-H-2, mixed leukocyte culture (MLC)-stimulating M-locus. Adoptive secondary in vivo antibody responses using immune spleen cells from mice of these strains demonstrate two interesting findings. One is a non-specific augmentation of the anti-hapten antibody response of CBA B cells by AKR T cells. This allogeneic effect is unidirectional in the same sense as the MLC between cells from these two strains. The second is that, despite an ongoing allogeneic effect, M-locus-incompatible T and B cells show strong specific cooperation. Thus, it seems unlikely that the failure of H-2-incompatible T and B cells to collaborate specifically is due to inhibitory allogenic reactions."}
{"id": "PMID:1085489", "title": "Medical records in family practice. A review.", "content": "The organisation of general practice in England is outlined and the independent contractor basis of the family practioner emphasised. Data from family practice, like data from hospital practice, may be used for clinical management, practice management, or research. Examples of applications in each of these fields are given. The basic records used in family practice--the medical record envelope, the prescription form and the claim for sickness benefit--are described. Some practices record morbidity (E Book or Diagnostic Index), some record systematically details of their activities (L Book or Activities Ledger) and some maintain age and sex registers and other registers of their patients; all these developments are outlined. Attention is drawn to the introduction of problem orientated records and to the use of computers in family practice, but these innovations are not discussed. Outstanding issues are the same as those in hospital record systems--accuracy, definitions, coverage, confidentiality, clerical support and costs, and the use made of the information.", "contents": "Medical records in family practice. A review. The organisation of general practice in England is outlined and the independent contractor basis of the family practioner emphasised. Data from family practice, like data from hospital practice, may be used for clinical management, practice management, or research. Examples of applications in each of these fields are given. The basic records used in family practice--the medical record envelope, the prescription form and the claim for sickness benefit--are described. Some practices record morbidity (E Book or Diagnostic Index), some record systematically details of their activities (L Book or Activities Ledger) and some maintain age and sex registers and other registers of their patients; all these developments are outlined. Attention is drawn to the introduction of problem orientated records and to the use of computers in family practice, but these innovations are not discussed. Outstanding issues are the same as those in hospital record systems--accuracy, definitions, coverage, confidentiality, clerical support and costs, and the use made of the information."}
{"id": "PMID:1085490", "title": "Multiple sclerosis and high incidence of a B lymphocyte antigen.", "content": "Multiple sclerosis patients were tested for six new antigens present on human B lymphocytes. The group 4 specificity occurred in 83.9% of the 56 patients as compared to 32.5 percent in 72 healthy controls (P less than .003). The antiserums defining the five other B lymphocyte specificities reacted at a lower frequency to B cells from multiple sclerosis patients, showing that increased reactivity to group 4 antiserum was specific. Linkage of a hypothesized multiple sclerosis susceptibility gene with certain haplotypes of HLA-A3, HLA-B7 HLA-DW2, and the new B group 4 can be inferred.", "contents": "Multiple sclerosis and high incidence of a B lymphocyte antigen. Multiple sclerosis patients were tested for six new antigens present on human B lymphocytes. The group 4 specificity occurred in 83.9% of the 56 patients as compared to 32.5 percent in 72 healthy controls (P less than .003). The antiserums defining the five other B lymphocyte specificities reacted at a lower frequency to B cells from multiple sclerosis patients, showing that increased reactivity to group 4 antiserum was specific. Linkage of a hypothesized multiple sclerosis susceptibility gene with certain haplotypes of HLA-A3, HLA-B7 HLA-DW2, and the new B group 4 can be inferred."}
{"id": "PMID:1085492", "title": "Aneurysms of the hepatic artery.", "content": "Aneurysms of the hepatic artery are rare and, when symptomatic, they may present a triad of upper abdominal pain, gastrointestinal bleeding, and obstructive jaundice. Asymptomatic and unsuspected aneurysms are demonstrated occasionally by abdominal arteriography or are encountered infrequently during abdominal operation for an unrelated disease. Hepatic artery aneurysms have great potential for rupture with bleeding into the peritioneal cavity, the common bile duct, or an adjacent hollow viscus. Morbidity and mortality with a ruptured aneurysm are high, and aggressive and imaginative operative therapy is required. Patients with an asymptomatic aneurysm should have operation to prevent subsequent rupture and hemorrhage.", "contents": "Aneurysms of the hepatic artery. Aneurysms of the hepatic artery are rare and, when symptomatic, they may present a triad of upper abdominal pain, gastrointestinal bleeding, and obstructive jaundice. Asymptomatic and unsuspected aneurysms are demonstrated occasionally by abdominal arteriography or are encountered infrequently during abdominal operation for an unrelated disease. Hepatic artery aneurysms have great potential for rupture with bleeding into the peritioneal cavity, the common bile duct, or an adjacent hollow viscus. Morbidity and mortality with a ruptured aneurysm are high, and aggressive and imaginative operative therapy is required. Patients with an asymptomatic aneurysm should have operation to prevent subsequent rupture and hemorrhage."}
{"id": "PMID:1085494", "title": "Lasiodiplodia theobromae as a cause of keratomycoses.", "content": "Four cases of human keratitis caused by the tropical fungus Lasiodiplodia theobromae have been encountered in Miami, Florida bringing to 8 the number of cases reported in the world literature. Two of the ulcers were mild. Three patients recovered without severe impairment of vision after topical polyene treatment, but 1 patient with a severe ulcer required therapeutic keratoplasty after 11 days of topical natamycin. Histopathology revealed fungus deep in the cornea, invading Descemet's membrane. L. theobromae appeared to have collagenase activity in vitro. Inoculation of L. theobromae into the corneas of rabbits produced progressive ulcers. The fungus was endemic in Miami on home grown and imported bananas. Polyene antimycotic antibiotics were fungicidal for L. theobromae in vitro. Thiabendazole was effectively fungistatic but varied in fungicidal effect. Clotrimazole and miconazole were only incompletely fungistatic. Of 7 strains of L. theobromae tested, 4 were relatively resistant to 5-flurocytosine.", "contents": "Lasiodiplodia theobromae as a cause of keratomycoses. Four cases of human keratitis caused by the tropical fungus Lasiodiplodia theobromae have been encountered in Miami, Florida bringing to 8 the number of cases reported in the world literature. Two of the ulcers were mild. Three patients recovered without severe impairment of vision after topical polyene treatment, but 1 patient with a severe ulcer required therapeutic keratoplasty after 11 days of topical natamycin. Histopathology revealed fungus deep in the cornea, invading Descemet's membrane. L. theobromae appeared to have collagenase activity in vitro. Inoculation of L. theobromae into the corneas of rabbits produced progressive ulcers. The fungus was endemic in Miami on home grown and imported bananas. Polyene antimycotic antibiotics were fungicidal for L. theobromae in vitro. Thiabendazole was effectively fungistatic but varied in fungicidal effect. Clotrimazole and miconazole were only incompletely fungistatic. Of 7 strains of L. theobromae tested, 4 were relatively resistant to 5-flurocytosine."}
{"id": "PMID:1085495", "title": "Experience with jejunoileal bypass for obesity.", "content": "Fifty-eight patients less than 30 years old but who are more than 300 pounds in weight were considered for investigation of jejunoileal bypass. Thirty patients had operations, and the functioning bypass included 40 centimeters of jejunum and 10 centimeters of ileum. In 14 patients, intestinal continuity was restored by either end-to-end or end-to-side anastomosis according to random choice, and in 16 patients, end-to-end anastomoses were used. At present, the follow-up period is one to four years, and the weight loss, so far, has not been significantly related to the type of reconstruction used. Weight loss correlated possitively with the preoperative weights up to one year after operation. Also, weight loss correlated positively with the total measured length of the small intestine during the first six postoperative months, but this correlation currently is showing a trend toward negativity. Food intake decreased by 2,682+/-690 calories per day at six months after operation as a result of the bypass. Chronic acidosis was common. Results of an endocrine evaluation revealed an unexpected significant decrease in parathormone levels within the normal range. A new complication, colonic pseudo-obstruction, has occurred one year or more after operation in five patients or 17 per cent of our group. This complication is related to the intestinal anaerobic flora in proximity of the defunctionalized limb; its symptoms and signs can be alleviated by giving antibiotics or Lactobacillus to change the intestinal flora. Although 90 per cent of our patients are pleased with their progress and the results of routine evaluation corroborate their satisfaction, detailed analysis of the research data available to us revealed that only 43 per cent have had beneficial effects from a jejunoileal bypass without any of the serious sequelae. We consider jejunoileal bypass for obesity justified when carried out by interdisciplinary groups dedicated to the long term follow-up study of the patient and periodic reporting of the results. The long term future for the operation is, as yet, not completely understood, and when the conditions we have recommended are not present, we cannot currently endorse the operation.", "contents": "Experience with jejunoileal bypass for obesity. Fifty-eight patients less than 30 years old but who are more than 300 pounds in weight were considered for investigation of jejunoileal bypass. Thirty patients had operations, and the functioning bypass included 40 centimeters of jejunum and 10 centimeters of ileum. In 14 patients, intestinal continuity was restored by either end-to-end or end-to-side anastomosis according to random choice, and in 16 patients, end-to-end anastomoses were used. At present, the follow-up period is one to four years, and the weight loss, so far, has not been significantly related to the type of reconstruction used. Weight loss correlated possitively with the preoperative weights up to one year after operation. Also, weight loss correlated positively with the total measured length of the small intestine during the first six postoperative months, but this correlation currently is showing a trend toward negativity. Food intake decreased by 2,682+/-690 calories per day at six months after operation as a result of the bypass. Chronic acidosis was common. Results of an endocrine evaluation revealed an unexpected significant decrease in parathormone levels within the normal range. A new complication, colonic pseudo-obstruction, has occurred one year or more after operation in five patients or 17 per cent of our group. This complication is related to the intestinal anaerobic flora in proximity of the defunctionalized limb; its symptoms and signs can be alleviated by giving antibiotics or Lactobacillus to change the intestinal flora. Although 90 per cent of our patients are pleased with their progress and the results of routine evaluation corroborate their satisfaction, detailed analysis of the research data available to us revealed that only 43 per cent have had beneficial effects from a jejunoileal bypass without any of the serious sequelae. We consider jejunoileal bypass for obesity justified when carried out by interdisciplinary groups dedicated to the long term follow-up study of the patient and periodic reporting of the results. The long term future for the operation is, as yet, not completely understood, and when the conditions we have recommended are not present, we cannot currently endorse the operation."}
{"id": "PMID:1085498", "title": "Immunologic studies in von Willebrand's disease: alteration of factor VIII/von Willebrand protein after transfusion with plasma concentrates in patients with von Willebrand's disease.", "content": "Two patients with a severe von Willebrand's disease characterized by no detectable factor VIII related antigen in their plasma received transfusions of cryoprecipitate. The bleeding time was corrected for a short period of time and returned to its pretransfusional value although the other parameters of the disease were still corrected. Electrophoretic and immunologic properties of factor VIII related antigen infused were determined serially after transfusion. Modifications of these properties occurred progressively after transfusion. The half disappearance time of F. VIII R.A. was determined and found to be considerably shorter than in hemophilic recipients. This study suggests an alteration in vivo of F. VIII R.A. infused into von Willebrand recipients.", "contents": "Immunologic studies in von Willebrand's disease: alteration of factor VIII/von Willebrand protein after transfusion with plasma concentrates in patients with von Willebrand's disease. Two patients with a severe von Willebrand's disease characterized by no detectable factor VIII related antigen in their plasma received transfusions of cryoprecipitate. The bleeding time was corrected for a short period of time and returned to its pretransfusional value although the other parameters of the disease were still corrected. Electrophoretic and immunologic properties of factor VIII related antigen infused were determined serially after transfusion. Modifications of these properties occurred progressively after transfusion. The half disappearance time of F. VIII R.A. was determined and found to be considerably shorter than in hemophilic recipients. This study suggests an alteration in vivo of F. VIII R.A. infused into von Willebrand recipients."}
{"id": "PMID:1085499", "title": "The platelet-endothelial cell--VIII axis.", "content": "Cultured human endothelial cells synthesize and secrete a protein(s) which has factor VIII antigen and von Willebrand factor activity. Subcellular membrane and granule fractions derived from human platelets also contain the factor VIII antigen and von Willebrand factor activity. Circulating platelets constitute a significant reservoir of VIII antigen containing approximately 15% of the amount present in platelet poor plasma. Thus normal platelets contain surface bound as well as intracellularly stored von Willebrand factor, a protein synthesized by endothelial cells which is required for normal platelet function.", "contents": "The platelet-endothelial cell--VIII axis. Cultured human endothelial cells synthesize and secrete a protein(s) which has factor VIII antigen and von Willebrand factor activity. Subcellular membrane and granule fractions derived from human platelets also contain the factor VIII antigen and von Willebrand factor activity. Circulating platelets constitute a significant reservoir of VIII antigen containing approximately 15% of the amount present in platelet poor plasma. Thus normal platelets contain surface bound as well as intracellularly stored von Willebrand factor, a protein synthesized by endothelial cells which is required for normal platelet function."}
{"id": "PMID:1085500", "title": "Importance of protease inhibition in studies on purified factor VIII (antihaemophilic factor).", "content": "According to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW approximately 2 X 10(6) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37 degrees C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macromolecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use.", "contents": "Importance of protease inhibition in studies on purified factor VIII (antihaemophilic factor). According to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW approximately 2 X 10(6) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37 degrees C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macromolecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use."}
{"id": "PMID:1085502", "title": "Induction of cytotoxic lymphocytes directed against public alloantigens.", "content": "The response of H-2k lymphocytes to in vitro stimulation by H-2b and HI2d spleen cells has been monitored for the production of cytotoxic lymphocytes (CL). Using cells from H-2b strains CL which were capable of lysing both H-2b and H-2d target cells (TC) were generated. These results, together with competitive inhibitor studies of the cytotoxic activity against H-2d TC, demonstrated that T lymphocytes can respond to both public and private alloantigens of the H-2 complex. When H-2d cells were used for stimulation two of the three strains tested induced CL with cytotoxic activity towards both H-2d and H-2b TC. These observations indicate that the T cell response to alloantigen, leading to the production of cytotoxic lymphocytes, can be multicomponent like the established B cell response and can be expected to be subject to as much phenotypic variation. The full complexity may only be revealed by examing a number of combinations of stimulating strains, methods of stimulation and different kinds of target cells.", "contents": "Induction of cytotoxic lymphocytes directed against public alloantigens. The response of H-2k lymphocytes to in vitro stimulation by H-2b and HI2d spleen cells has been monitored for the production of cytotoxic lymphocytes (CL). Using cells from H-2b strains CL which were capable of lysing both H-2b and H-2d target cells (TC) were generated. These results, together with competitive inhibitor studies of the cytotoxic activity against H-2d TC, demonstrated that T lymphocytes can respond to both public and private alloantigens of the H-2 complex. When H-2d cells were used for stimulation two of the three strains tested induced CL with cytotoxic activity towards both H-2d and H-2b TC. These observations indicate that the T cell response to alloantigen, leading to the production of cytotoxic lymphocytes, can be multicomponent like the established B cell response and can be expected to be subject to as much phenotypic variation. The full complexity may only be revealed by examing a number of combinations of stimulating strains, methods of stimulation and different kinds of target cells."}
{"id": "PMID:1085503", "title": "Colony-forming cells in culture and colony-stimulating activity of the urine and the serum in a case of cyclic neutropenia.", "content": "A 22-year-old female with cyclic neutropenia was studied. Her bone marrow cells showed high colony-forming activity in soft agar through the cycle, though there were slight fluctuations in the number and the shape of colonies. On the other hand, the peak of urinary colong-stimulating activity (CSA) occurred at the neutropenic stage. The dialyzed serum showed two peaks of CSA, i.e., at the peak and the valley of the neutrophil count, although the undialyzed serum showed only one peak at the neutroenic stage. It is suggested on the basis of these data that humoral factors may play a role in maintaining the neutrophil cycle.", "contents": "Colony-forming cells in culture and colony-stimulating activity of the urine and the serum in a case of cyclic neutropenia. A 22-year-old female with cyclic neutropenia was studied. Her bone marrow cells showed high colony-forming activity in soft agar through the cycle, though there were slight fluctuations in the number and the shape of colonies. On the other hand, the peak of urinary colong-stimulating activity (CSA) occurred at the neutropenic stage. The dialyzed serum showed two peaks of CSA, i.e., at the peak and the valley of the neutrophil count, although the undialyzed serum showed only one peak at the neutroenic stage. It is suggested on the basis of these data that humoral factors may play a role in maintaining the neutrophil cycle."}
{"id": "PMID:1085504", "title": "Alpha1-antichymotrypsin in rheumatoid arthritis.", "content": "Serum alpha1-antichymotrypsin (alpha1-ACT) of the patient with rheumatoid arthritis was studied by means of single radial immunodiffusion method. There was a significant elevation of the alpha1-ACT concentration in the patients with rheumatoid arthritis, and positive relationships were observed between the concentrations of alpha1-ACT and of other glycoproteins such as alpha1-acid glycoprotein and alpha1-antitrypsin in individual patients, and between C-reactive protein (CRP) rates and the (CRP) rates and the alpha1-ACT concentrations in individual specimens. These facts suggest that alpha1-ACT belongs to a group of acute phase proteins like alpha1-antitrypsin or CRP. An inverse proportional correlation was revealed between alpha1-ACT and fibrinolytic activity. No influences were observed on the alpha1-ACT concentration, activity index or articular index by the oral administration of alpha-chymotrypsin in patients with rheumatoid arthritis.", "contents": "Alpha1-antichymotrypsin in rheumatoid arthritis. Serum alpha1-antichymotrypsin (alpha1-ACT) of the patient with rheumatoid arthritis was studied by means of single radial immunodiffusion method. There was a significant elevation of the alpha1-ACT concentration in the patients with rheumatoid arthritis, and positive relationships were observed between the concentrations of alpha1-ACT and of other glycoproteins such as alpha1-acid glycoprotein and alpha1-antitrypsin in individual patients, and between C-reactive protein (CRP) rates and the (CRP) rates and the alpha1-ACT concentrations in individual specimens. These facts suggest that alpha1-ACT belongs to a group of acute phase proteins like alpha1-antitrypsin or CRP. An inverse proportional correlation was revealed between alpha1-ACT and fibrinolytic activity. No influences were observed on the alpha1-ACT concentration, activity index or articular index by the oral administration of alpha-chymotrypsin in patients with rheumatoid arthritis."}
{"id": "PMID:1085509", "title": "Drug effects on the fine structure of Trypanosoma rhodesiense: puromycin and its aminonucleoside, Cordycepin and Nucleocidin.", "content": "The effects of four trypanocidal adenine nucleosides on the fine structure of a monomorphic strain of Trypanosoma rhodesiense have been examined. All four drugs, Puromycin and its aminonucleoside, Cordycepin and Nucleocidin, induced electron-lucent cytoplasmic clefts in the cytoplasm, which were generally acicular or spindle-shaped, with long axes lying in any direction. The clefts had an intimate relationship with the rough endoplasmic reticulum. The drugs also produced excessive lysosomal vacuolation, and two, Cordycepin and Nucleocidin, caused nucleolar fragmentation and probable segregation, indicative of interference with RNA synthesis. The significance of the lesions is discussed in relation to known properties of adenine nucleoside drugs.", "contents": "Drug effects on the fine structure of Trypanosoma rhodesiense: puromycin and its aminonucleoside, Cordycepin and Nucleocidin. The effects of four trypanocidal adenine nucleosides on the fine structure of a monomorphic strain of Trypanosoma rhodesiense have been examined. All four drugs, Puromycin and its aminonucleoside, Cordycepin and Nucleocidin, induced electron-lucent cytoplasmic clefts in the cytoplasm, which were generally acicular or spindle-shaped, with long axes lying in any direction. The clefts had an intimate relationship with the rough endoplasmic reticulum. The drugs also produced excessive lysosomal vacuolation, and two, Cordycepin and Nucleocidin, caused nucleolar fragmentation and probable segregation, indicative of interference with RNA synthesis. The significance of the lesions is discussed in relation to known properties of adenine nucleoside drugs."}
{"id": "PMID:1085508", "title": "Acute gastric changes in patients with acute stroke. Part 1: with reference to gastroendoscopic findings.", "content": "A gastroendoscopic study was performed on 177 patients with acute stroke. Gastric changes were found in 92 among them (52%), including ten with acute ulcer. A high frequency of gastric changes was found in patients with serious stroke and/or in patients whose cerebral lesions were located close to the hypothalamus or its centrifugal tract. The mortality in patients with gastric changes was high, especially in patients with acute ulcers, multiple erosions and petechiae. The frequency of brown gastric juice and coffee grounds at gastroendoscopic examination (gastric hemorrhage) was three times as high as that of melena and/or hematoemesis. On the other hand, the mortality of patients with gastric hemorrhage differed little from that of patients with melena and/or hematoemesis.", "contents": "Acute gastric changes in patients with acute stroke. Part 1: with reference to gastroendoscopic findings. A gastroendoscopic study was performed on 177 patients with acute stroke. Gastric changes were found in 92 among them (52%), including ten with acute ulcer. A high frequency of gastric changes was found in patients with serious stroke and/or in patients whose cerebral lesions were located close to the hypothalamus or its centrifugal tract. The mortality in patients with gastric changes was high, especially in patients with acute ulcers, multiple erosions and petechiae. The frequency of brown gastric juice and coffee grounds at gastroendoscopic examination (gastric hemorrhage) was three times as high as that of melena and/or hematoemesis. On the other hand, the mortality of patients with gastric hemorrhage differed little from that of patients with melena and/or hematoemesis."}
{"id": "PMID:1085507", "title": "Cerebellar hemorrhage: reliability of clinical evaluation.", "content": "Surgery without confirmatory radiological contrast studies has been advocated in patients suspected of having a cerebellar hemorrhage. To determine the reliabiligy of the clinical evaluation, records were reviewed of 33 patients in whom the diagnosis of cerebellar hemorrhage was initially suspected or subsequently confirmed. In 13 patients the correct diagnosis had been made initially by clinical evaluation, but in ten patients the correct diagnosis was not suspected at the initial evaluation. The remaining ten patients had typical backgrounds, symptoms, and signs of a spontaneous cerebellar hemorrhage, but radiological contrast studies and clinical observation indicated that other neurological lesions were responsible. Thus, utilizing solely the clinical evaluation, the diagnosis of cerebellar hemorrhage may be made only with difficulty and not with the certainty required for emergency surgery.", "contents": "Cerebellar hemorrhage: reliability of clinical evaluation. Surgery without confirmatory radiological contrast studies has been advocated in patients suspected of having a cerebellar hemorrhage. To determine the reliabiligy of the clinical evaluation, records were reviewed of 33 patients in whom the diagnosis of cerebellar hemorrhage was initially suspected or subsequently confirmed. In 13 patients the correct diagnosis had been made initially by clinical evaluation, but in ten patients the correct diagnosis was not suspected at the initial evaluation. The remaining ten patients had typical backgrounds, symptoms, and signs of a spontaneous cerebellar hemorrhage, but radiological contrast studies and clinical observation indicated that other neurological lesions were responsible. Thus, utilizing solely the clinical evaluation, the diagnosis of cerebellar hemorrhage may be made only with difficulty and not with the certainty required for emergency surgery."}
{"id": "PMID:1085512", "title": "Progressive human cone-rod dysfunction (dystrophy).", "content": "The author has classified progressive human cone-rod dysfunction into primary and secondary types. The primary type, identified by early ERG cone and usually also rod abnormalities, was further subdivided into types 1 and 2 based on, amoung other distinguishing characteristics, the extent of associated retinal pigment epithelial defects. Secondary cone-rod dysfunction apparently results from disease initially affecting the retinal pigment epithelium. Initially normal ERG findings and the presence of flecks characterize this (type 3) progressive cone-rod dysfunction.", "contents": "Progressive human cone-rod dysfunction (dystrophy). The author has classified progressive human cone-rod dysfunction into primary and secondary types. The primary type, identified by early ERG cone and usually also rod abnormalities, was further subdivided into types 1 and 2 based on, amoung other distinguishing characteristics, the extent of associated retinal pigment epithelial defects. Secondary cone-rod dysfunction apparently results from disease initially affecting the retinal pigment epithelium. Initially normal ERG findings and the presence of flecks characterize this (type 3) progressive cone-rod dysfunction."}
{"id": "PMID:1085517", "title": "[Relaparotomy in acute hemorrhage after operations on the abdominal organs].", "content": "In the paper, an analysis of acute postoperative hemorrhages in 30 patients after operations on the abdomen is presented. In these patients 42 relaparatomies have been performed with lethal issues in 16.7%. The causes of this complication are analysed, and recommendations how to prevent them are given.", "contents": "[Relaparotomy in acute hemorrhage after operations on the abdominal organs]. In the paper, an analysis of acute postoperative hemorrhages in 30 patients after operations on the abdomen is presented. In these patients 42 relaparatomies have been performed with lethal issues in 16.7%. The causes of this complication are analysed, and recommendations how to prevent them are given."}
{"id": "PMID:1085518", "title": "[Retrograde mammary-coronary anastomosis and its place among other methods of myocardial revascularization].", "content": "Under consideration is a special kind of mammary-coronary anastomosis--a retrograde mammary-coronary anastomosis, first suggested by the authors. An anteretrograde mammary-coronary anastomosis was performed in 104 patients, a retrograde one--in 6. The use of a retrograde mammary-coronary anastomosis yielded favourable results, which in some cases are remained for 4-5 years.", "contents": "[Retrograde mammary-coronary anastomosis and its place among other methods of myocardial revascularization]. Under consideration is a special kind of mammary-coronary anastomosis--a retrograde mammary-coronary anastomosis, first suggested by the authors. An anteretrograde mammary-coronary anastomosis was performed in 104 patients, a retrograde one--in 6. The use of a retrograde mammary-coronary anastomosis yielded favourable results, which in some cases are remained for 4-5 years."}
{"id": "PMID:1085521", "title": "[Immune response of the chicks from immune layers vaccinated at 1 and 4 days of age against Newcastle disease].", "content": "The study of antihemagglutinins in day-old chicks revealed the presence of congenital antibodies against the Newcastle disease virus, having an average geometric titer of lg2 7.9. In the vaccinated 1,500 chicks on the first and fourth day with a La Sota vaccine there was four days later a drop in the antibody titer. Then again a rise in the antibody titer was noted that reached on the 14th day an average geometric value of lg2 7.6 which remained unchanged up to the 40th day. The antihemagglutination titers in the untreated chicks on the 40th day of age dropped to lg2 1.8. As many as 70 per cent of the control chicks died at challenging on the 24th day of age, while all of the treated birds (100 per cent) were given protection through vaccination by the spray method or ocularly and nasally, withstanding challenge on the 20th and 24th day following treatment.", "contents": "[Immune response of the chicks from immune layers vaccinated at 1 and 4 days of age against Newcastle disease]. The study of antihemagglutinins in day-old chicks revealed the presence of congenital antibodies against the Newcastle disease virus, having an average geometric titer of lg2 7.9. In the vaccinated 1,500 chicks on the first and fourth day with a La Sota vaccine there was four days later a drop in the antibody titer. Then again a rise in the antibody titer was noted that reached on the 14th day an average geometric value of lg2 7.6 which remained unchanged up to the 40th day. The antihemagglutination titers in the untreated chicks on the 40th day of age dropped to lg2 1.8. As many as 70 per cent of the control chicks died at challenging on the 24th day of age, while all of the treated birds (100 per cent) were given protection through vaccination by the spray method or ocularly and nasally, withstanding challenge on the 20th and 24th day following treatment."}
{"id": "PMID:1085531", "title": "[Quantitative determination of alpha1-antitrypsin in lung tissue from adults with emphysema and newborns with respiratory-distress-syndrome (author's transl)].", "content": "In a small number of patients, lung emphysema can be attributed to a remarkable deficiency of alpha1-antitrypsin. Concentrations reduced about 50% of the normal value represent a predisposing factor. In most cases of newborns with respiratory-distress-syndrome the concentration of alpha1-antitrypsin is decreased. Its determination can be used for the early diagnosis of the respiratory-distress-syndrome.", "contents": "[Quantitative determination of alpha1-antitrypsin in lung tissue from adults with emphysema and newborns with respiratory-distress-syndrome (author's transl)]. In a small number of patients, lung emphysema can be attributed to a remarkable deficiency of alpha1-antitrypsin. Concentrations reduced about 50% of the normal value represent a predisposing factor. In most cases of newborns with respiratory-distress-syndrome the concentration of alpha1-antitrypsin is decreased. Its determination can be used for the early diagnosis of the respiratory-distress-syndrome."}
{"id": "PMID:1085532", "title": "[Quantitative determination of blood loss by means of 51 Cr-labeled erythrocytes in occult hemorrhages of the gastrointestinal tract].", "content": "By means of 51 Cr-labelled erythrocytes the stools of 17 patients were examined for occult admixtures of blood. Hereby a method well suited for routine work was developed, the manipulations with stool were reduced to a minimum measure. As measuring device an animal total body counter was used. The average excretion of blood in persons without diseases of the gastro-intestinal tract was 0.49+/- 0.38 ml/d. In three patients a haemorrhage could be proved. The quantitative determination of blood losses through the intestine is diagnostically valuable. It is useful particularly in unclear iron deficiency anaemis. A combination of the method with the determination of the survival time of erythrocytes is possible.", "contents": "[Quantitative determination of blood loss by means of 51 Cr-labeled erythrocytes in occult hemorrhages of the gastrointestinal tract]. By means of 51 Cr-labelled erythrocytes the stools of 17 patients were examined for occult admixtures of blood. Hereby a method well suited for routine work was developed, the manipulations with stool were reduced to a minimum measure. As measuring device an animal total body counter was used. The average excretion of blood in persons without diseases of the gastro-intestinal tract was 0.49+/- 0.38 ml/d. In three patients a haemorrhage could be proved. The quantitative determination of blood losses through the intestine is diagnostically valuable. It is useful particularly in unclear iron deficiency anaemis. A combination of the method with the determination of the survival time of erythrocytes is possible."}
{"id": "PMID:1085533", "title": "[Behavior of thymus-arised lymphatic cells in the peripheral blood in acute leukoses].", "content": "On the basis of their ability of the spontaneous formation of rosettes by unsensibilised sheep erythrocytes the proportion of thymic lymphatic cells in 7 acute and 3 chronic lymphatic leukaemias was determined. Further observations on the content of T-cells in the peripheral blood were performed in leukoses, whereby the remission was begun with prednisone and cytostatics, under the immune stimulation with BCG during the remission and in 3 children with lymphogranulomatosis before and after splenectomy. The results of the acute stage of leucosis refer to the possibility of a prognostic evidence with the subclassification of the ALL. The T-cell-leukaemias revealed a particularly unfavourable course of disease. Cytochemically they were acute lymphatic leukaemias of undifferentiated type. In leucoses with smaller proportion of T-cells the blasts above all revealed a granular PAS-reaction.", "contents": "[Behavior of thymus-arised lymphatic cells in the peripheral blood in acute leukoses]. On the basis of their ability of the spontaneous formation of rosettes by unsensibilised sheep erythrocytes the proportion of thymic lymphatic cells in 7 acute and 3 chronic lymphatic leukaemias was determined. Further observations on the content of T-cells in the peripheral blood were performed in leukoses, whereby the remission was begun with prednisone and cytostatics, under the immune stimulation with BCG during the remission and in 3 children with lymphogranulomatosis before and after splenectomy. The results of the acute stage of leucosis refer to the possibility of a prognostic evidence with the subclassification of the ALL. The T-cell-leukaemias revealed a particularly unfavourable course of disease. Cytochemically they were acute lymphatic leukaemias of undifferentiated type. In leucoses with smaller proportion of T-cells the blasts above all revealed a granular PAS-reaction."}
{"id": "PMID:1085534", "title": "[The antithrombotic treatment and prevention of recurrence with acetylsalicylic acid in myocardial infarct].", "content": "A comparison of the therapy, especially of the long-term recidivation prophylaxis of the myocardial infarction with acetyl salicylic acid and anticoagulants, on the basis of 314 observations allows of the following opinions: By means of the thrombocyte aggregation inhibitor acetyl salicylic acid an antithrombotic therapy and recidivation prophylaxis of good value can be performed. The application of acetyl salicylic acid is, concerning the course of the disease after the onset of the infarction and the frequency of recidivation, adequate to the treatment with classical anticoagulants. The practical performance of the long-term therapy with acetyl salicylic acid is significantly simplified for physician and patient compared with the application of anticoagulants. The daily dose lies uniformly at 1.5 g. There is no fear of acute haemorrhagic complications. As control only the occasional establishment of the Hb-value as well as of the time of haemorrhage is indicated. The only, however, very decisive disadvantage of the acetyl salicylic acid, the bad compatibility for the stomach, is certainly not abolished with the new drug \"Micristin\", but much diminished.", "contents": "[The antithrombotic treatment and prevention of recurrence with acetylsalicylic acid in myocardial infarct]. A comparison of the therapy, especially of the long-term recidivation prophylaxis of the myocardial infarction with acetyl salicylic acid and anticoagulants, on the basis of 314 observations allows of the following opinions: By means of the thrombocyte aggregation inhibitor acetyl salicylic acid an antithrombotic therapy and recidivation prophylaxis of good value can be performed. The application of acetyl salicylic acid is, concerning the course of the disease after the onset of the infarction and the frequency of recidivation, adequate to the treatment with classical anticoagulants. The practical performance of the long-term therapy with acetyl salicylic acid is significantly simplified for physician and patient compared with the application of anticoagulants. The daily dose lies uniformly at 1.5 g. There is no fear of acute haemorrhagic complications. As control only the occasional establishment of the Hb-value as well as of the time of haemorrhage is indicated. The only, however, very decisive disadvantage of the acetyl salicylic acid, the bad compatibility for the stomach, is certainly not abolished with the new drug \"Micristin\", but much diminished."}
{"id": "PMID:1085535", "title": "[Studies on the frequency and diagnostic value of hepatomegaly].", "content": "In 4 groups of patients (I = 1 621 internistic in-patients, II = 2 073 donors with increased SGPT-values, III = 2 619 after-examined patients with hepatitis, IV = 514 patients of a sanatorium for liver diseases) frequency and diagnostic validity of the symptom enlargement of the liver are tested. The most frequent cause of hepatomegalia is in group I an insufficiency of the right heart. The highest percentage, however, is to be found in liver diseases, then come heart insufficiency, diseases of stomach and gall-bladder, diabetes, rheumatic diseases. In these diseases liver biopsy detects additional changes in many cases. The validity test in the 4 groups yields very different results, dependent on the number of patients and the diagnostic questioning. The symptom hepatomegalia cannot be used as a screening test, but it is of high diagnostic value in the clinical diagnostics.", "contents": "[Studies on the frequency and diagnostic value of hepatomegaly]. In 4 groups of patients (I = 1 621 internistic in-patients, II = 2 073 donors with increased SGPT-values, III = 2 619 after-examined patients with hepatitis, IV = 514 patients of a sanatorium for liver diseases) frequency and diagnostic validity of the symptom enlargement of the liver are tested. The most frequent cause of hepatomegalia is in group I an insufficiency of the right heart. The highest percentage, however, is to be found in liver diseases, then come heart insufficiency, diseases of stomach and gall-bladder, diabetes, rheumatic diseases. In these diseases liver biopsy detects additional changes in many cases. The validity test in the 4 groups yields very different results, dependent on the number of patients and the diagnostic questioning. The symptom hepatomegalia cannot be used as a screening test, but it is of high diagnostic value in the clinical diagnostics."}
{"id": "PMID:1085536", "title": "[Prerequisites and possibilities of immunotherapy in acute leukemias].", "content": "Nowadays there are references that immuno-reactions of patients with carcinoma against tumour-associated antigens are of essential significance for the development and progressing of tumours. Also the frequent appearance of neoplasms in primary immunodeficiency syndromes indicates this fact. Also in human leukaemias there exist leukaemia-associated antigens. Humoral as well as cell-bound immunoreactions may be proved. Possibly leukaemia-associated immunoglobulins are blocking factors which prevent the cellular immunoreactions against leukaemia-associated membrane antigens. The presence of specific reactions against antigens on leukaemia-cells was the condition for a successful immunotherapy. Despite many theoretical possibilities the immunotherapy in the clinic at present restricts to the BCG-vaccination and to the application of living or irradiated leukaemia-cells. The hitherto reported results are encouraging.", "contents": "[Prerequisites and possibilities of immunotherapy in acute leukemias]. Nowadays there are references that immuno-reactions of patients with carcinoma against tumour-associated antigens are of essential significance for the development and progressing of tumours. Also the frequent appearance of neoplasms in primary immunodeficiency syndromes indicates this fact. Also in human leukaemias there exist leukaemia-associated antigens. Humoral as well as cell-bound immunoreactions may be proved. Possibly leukaemia-associated immunoglobulins are blocking factors which prevent the cellular immunoreactions against leukaemia-associated membrane antigens. The presence of specific reactions against antigens on leukaemia-cells was the condition for a successful immunotherapy. Despite many theoretical possibilities the immunotherapy in the clinic at present restricts to the BCG-vaccination and to the application of living or irradiated leukaemia-cells. The hitherto reported results are encouraging."}
{"id": "PMID:1085537", "title": "[Endoscopy of postoperative hemorrhage in the upper gastrointestinal tract].", "content": "In hemorrhages from the upper gastrointestinal tract, emergency endoscopy is becoming more and more significant. Hemorrhages during the early postoperative stage, particularly in abdominal surgery, require particular attention due to the generally difficult postoperative situation. In most instances, intragastral hemorrhages are due to erosions and anastomotic bleeding. A detailed knowledge of the surgery performed may contribute to a quick and successful examination. Among the most important premises for a high rate of correct diagnoses are the experience of the examining physician as well as an early or immediate identification of the indications. In addition to the diagnostic significance, under favorable circumstances endoscopy makes possible definitive or temporary staunching of the bleeding by means of diathermy, laser beam or sclerotization.", "contents": "[Endoscopy of postoperative hemorrhage in the upper gastrointestinal tract]. In hemorrhages from the upper gastrointestinal tract, emergency endoscopy is becoming more and more significant. Hemorrhages during the early postoperative stage, particularly in abdominal surgery, require particular attention due to the generally difficult postoperative situation. In most instances, intragastral hemorrhages are due to erosions and anastomotic bleeding. A detailed knowledge of the surgery performed may contribute to a quick and successful examination. Among the most important premises for a high rate of correct diagnoses are the experience of the examining physician as well as an early or immediate identification of the indications. In addition to the diagnostic significance, under favorable circumstances endoscopy makes possible definitive or temporary staunching of the bleeding by means of diathermy, laser beam or sclerotization."}
{"id": "PMID:1085538", "title": "[Abdominal roetgen diagnosis in intensive care patients].", "content": "Following the discussion on special clinical issues with respect to patients in surgical and internal intensive care wards, technology and special methods used in conventional thorax and abdominal X-rays are elaborated on. The wide range of information obtained through large spectrum abdominal X-rays taken in the ventrodorsal direction with the patient lying on the left side is stressed. Small quantities of free air are visualized better in this position than in the upright one. The importance of additional X-rays of thorax and epigatrium in a seated position is accented. Subject of discussion is also the indication for visualizing by contrast medium and the selection of a contrast medium with due consideration of clinical criteria. The urgent need of an X-ray diagnosis in such a group of patients requires high standards in terms of techniques and methods used for examination--something that lies within the realm of the radiologist. Close cooperation with the attending team of physicians is a must if optimum interpretation of the X-rays is to be guaranteed.", "contents": "[Abdominal roetgen diagnosis in intensive care patients]. Following the discussion on special clinical issues with respect to patients in surgical and internal intensive care wards, technology and special methods used in conventional thorax and abdominal X-rays are elaborated on. The wide range of information obtained through large spectrum abdominal X-rays taken in the ventrodorsal direction with the patient lying on the left side is stressed. Small quantities of free air are visualized better in this position than in the upright one. The importance of additional X-rays of thorax and epigatrium in a seated position is accented. Subject of discussion is also the indication for visualizing by contrast medium and the selection of a contrast medium with due consideration of clinical criteria. The urgent need of an X-ray diagnosis in such a group of patients requires high standards in terms of techniques and methods used for examination--something that lies within the realm of the radiologist. Close cooperation with the attending team of physicians is a must if optimum interpretation of the X-rays is to be guaranteed."}
{"id": "PMID:1085549", "title": "[Mechanism of the effect of sera from rats subjected to high external temperature on rosette formation by splenic lymphocytes from non-immune animals].", "content": "Serum of rats subjected to the action of moderately high external temperature increased the immunogenicity of heterologous erythrocytes, but became inactive at a temperature of 2 degrees C and after the treatment with a polyvalent proteinase inhibitor--trasilol. Incubation of sheep erythrocytes with the serum of heated rats intensified the reaction of rosette formation by the lymphocytes of the spleen of intact rats. Incubation of the spleen cells with the sera of intact and heated rats failed to influence the rosette formation. After trasilol treatment the serum of heated rats completely lost the property to intensify the reaction of rosette formation. Phenomenon of intensification of rosette formation was revealed in case of treatment of erythrocytes with the sera at a temperature of 37 degrees C and was not reproduced at a temperature of 2 degrees C. Investigations carried out indicated that the increase in the immunogenicity of sheep erythrocytes and the intensification of their binding with the lymphocyte receptors of intact rats under the effect of the serum of heated animals were caused by the action of the same factor.", "contents": "[Mechanism of the effect of sera from rats subjected to high external temperature on rosette formation by splenic lymphocytes from non-immune animals]. Serum of rats subjected to the action of moderately high external temperature increased the immunogenicity of heterologous erythrocytes, but became inactive at a temperature of 2 degrees C and after the treatment with a polyvalent proteinase inhibitor--trasilol. Incubation of sheep erythrocytes with the serum of heated rats intensified the reaction of rosette formation by the lymphocytes of the spleen of intact rats. Incubation of the spleen cells with the sera of intact and heated rats failed to influence the rosette formation. After trasilol treatment the serum of heated rats completely lost the property to intensify the reaction of rosette formation. Phenomenon of intensification of rosette formation was revealed in case of treatment of erythrocytes with the sera at a temperature of 37 degrees C and was not reproduced at a temperature of 2 degrees C. Investigations carried out indicated that the increase in the immunogenicity of sheep erythrocytes and the intensification of their binding with the lymphocyte receptors of intact rats under the effect of the serum of heated animals were caused by the action of the same factor."}
{"id": "PMID:1085543", "title": "Bypass to anterior descending coronary artery in patients with aneurysm or scarring of left ventricle.", "content": "Fifteen cases who have had myocardial infarction with aneurysm formation or severe scarring of the left ventricle as a result of occlusion of left anterior descending coronary artery are presented. Bypass to left anterior descending coronary artery using internal mammary artery or saphenous vein was done in all of these patients. Ten of these had plication or excision of the left ventricular aneurysm. All of them survived the operation and are doing well. Early results in this series of patients indicate that left anterior descending coronary artery bypass in these patients is of great value by allowing perfusion to patent brances of left anterior descending coronary artery.", "contents": "Bypass to anterior descending coronary artery in patients with aneurysm or scarring of left ventricle. Fifteen cases who have had myocardial infarction with aneurysm formation or severe scarring of the left ventricle as a result of occlusion of left anterior descending coronary artery are presented. Bypass to left anterior descending coronary artery using internal mammary artery or saphenous vein was done in all of these patients. Ten of these had plication or excision of the left ventricular aneurysm. All of them survived the operation and are doing well. Early results in this series of patients indicate that left anterior descending coronary artery bypass in these patients is of great value by allowing perfusion to patent brances of left anterior descending coronary artery."}
{"id": "PMID:1085550", "title": "[Perforation of the interventricular septum, a complication of myocardial infarct].", "content": "The authors report nine surgical attempts in ventricular septal rupture following myocardial infarction and compare their experience with data from the literature. Two of the three long-term successes have been obtained by the association of infarcectomy and myocardial revascularisation to the repair of the septal rupture.", "contents": "[Perforation of the interventricular septum, a complication of myocardial infarct]. The authors report nine surgical attempts in ventricular septal rupture following myocardial infarction and compare their experience with data from the literature. Two of the three long-term successes have been obtained by the association of infarcectomy and myocardial revascularisation to the repair of the septal rupture."}
{"id": "PMID:1085551", "title": "[Cardio-respiratory assistance with the extracorporeal membrane oxygenator for massive pulmonary embolism].", "content": "A 39 year old pneumectomized patient presents a massive pulmonary embolism, dies within 3 hours and is supported inefficiently by cardiac massage with recurrent mydriasis during 2 hours. At that time, under extracorporeal cardiopulmonary bypass with a membrane oxygenator, the cardiac activity recovers immediatly due to right decompression and coronary perfusion. The patient is conscious within 5 hours. The cardiopulmonary bypass with a membrane oxygenator appears to be the best therapy when the cardiac massage fails to restitute a normal myocardial function. No embolectomy was performed. The patient died when the bypass was stopped after 48 hours. We conclude that the prolonged peripheral extracorporeal bypass followed by embolectomy is the best therapy of pulmonary embolism.", "contents": "[Cardio-respiratory assistance with the extracorporeal membrane oxygenator for massive pulmonary embolism]. A 39 year old pneumectomized patient presents a massive pulmonary embolism, dies within 3 hours and is supported inefficiently by cardiac massage with recurrent mydriasis during 2 hours. At that time, under extracorporeal cardiopulmonary bypass with a membrane oxygenator, the cardiac activity recovers immediatly due to right decompression and coronary perfusion. The patient is conscious within 5 hours. The cardiopulmonary bypass with a membrane oxygenator appears to be the best therapy when the cardiac massage fails to restitute a normal myocardial function. No embolectomy was performed. The patient died when the bypass was stopped after 48 hours. We conclude that the prolonged peripheral extracorporeal bypass followed by embolectomy is the best therapy of pulmonary embolism."}
{"id": "PMID:1085552", "title": "Single right ventricle (double-inlet right ventricle). A pathological specimen with comments on embryology.", "content": "This paper describes a pathological specimen of double-inlet right ventricle in which no remains of a hypoplastic left ventricle were found. An ontogenetic explanation of the malformation is presented based on the development of the normal human embryos. This case may be an example of exaggerated displacement of the atrioventricular canal towards the bulbus cordis in which the left ventricular cavity is extremely hypoplastic or aplastic and is not recognizable. The concept of early and late displacement is introduced.", "contents": "Single right ventricle (double-inlet right ventricle). A pathological specimen with comments on embryology. This paper describes a pathological specimen of double-inlet right ventricle in which no remains of a hypoplastic left ventricle were found. An ontogenetic explanation of the malformation is presented based on the development of the normal human embryos. This case may be an example of exaggerated displacement of the atrioventricular canal towards the bulbus cordis in which the left ventricular cavity is extremely hypoplastic or aplastic and is not recognizable. The concept of early and late displacement is introduced."}
{"id": "PMID:1085553", "title": "[Complete atrioventricular block in bacterial endocarditis].", "content": "A case of bacterial endocarditis complicated by complete auriculoventricular block is reported. The pathological findings and data from previously reported cases point to the fact that this complication is generally indication of very extensive cardiac and valvular damage. Surgery, although dangerous, should be attempted in the circumstances.", "contents": "[Complete atrioventricular block in bacterial endocarditis]. A case of bacterial endocarditis complicated by complete auriculoventricular block is reported. The pathological findings and data from previously reported cases point to the fact that this complication is generally indication of very extensive cardiac and valvular damage. Surgery, although dangerous, should be attempted in the circumstances."}
{"id": "PMID:1085555", "title": "Cilia-forming potentials of endocrine organs originating from the foregut.", "content": "Cilia were found in intracellular localizations or on the surface of certain cells of frog thymus and chicken parathyroid gland and ultimobranchial body. The experimental observations suggest that ciliogenesis is probably a general property of branchiogenic epithelium, which either persists, or can be activated by certain influences after the functional differentiation of endocrine organs arising from the foregut.", "contents": "Cilia-forming potentials of endocrine organs originating from the foregut. Cilia were found in intracellular localizations or on the surface of certain cells of frog thymus and chicken parathyroid gland and ultimobranchial body. The experimental observations suggest that ciliogenesis is probably a general property of branchiogenic epithelium, which either persists, or can be activated by certain influences after the functional differentiation of endocrine organs arising from the foregut."}
{"id": "PMID:1085556", "title": "Ampicllin sensitivity and biotypes of recent Danish isolates of Haemophilus influenzae.", "content": "Among 148 strains of Haemophilus influenzae isolated in 1975 from different localities in Denmark, none was found to be resistant to ampicillin. A majority of the strains belonged to biotypes I, II and III and the presence of a partial correlation between biotype and origin was confirmed.", "contents": "Ampicllin sensitivity and biotypes of recent Danish isolates of Haemophilus influenzae. Among 148 strains of Haemophilus influenzae isolated in 1975 from different localities in Denmark, none was found to be resistant to ampicillin. A majority of the strains belonged to biotypes I, II and III and the presence of a partial correlation between biotype and origin was confirmed."}
{"id": "PMID:1085557", "title": "The relationship between pregnancy, HCS and B lymphocytes.", "content": "The quantitative distribution of the lymphocyte subpopulations (B, T and null) and the serum concentrations of Human Chorionic Somatomammotropin (HCS), immunoglobulins IgG, IgM and IgA, complement component C4 and C1 inactivator, were estimated in venous blood samples from 32 women at various stages of pregnancy and compared with a control series of 7 non-pregnant normal women. A significant decline in the B cell percentage during pregnancy was seen. The fall in the percentage of the B lymphocytes was found to be concomitant with the rise in HCS concentration. No significant changes in the other parameters studied were present.", "contents": "The relationship between pregnancy, HCS and B lymphocytes. The quantitative distribution of the lymphocyte subpopulations (B, T and null) and the serum concentrations of Human Chorionic Somatomammotropin (HCS), immunoglobulins IgG, IgM and IgA, complement component C4 and C1 inactivator, were estimated in venous blood samples from 32 women at various stages of pregnancy and compared with a control series of 7 non-pregnant normal women. A significant decline in the B cell percentage during pregnancy was seen. The fall in the percentage of the B lymphocytes was found to be concomitant with the rise in HCS concentration. No significant changes in the other parameters studied were present."}
{"id": "PMID:1085558", "title": "Intracranial pressure during ventriculography using meglumine iothalamate.", "content": "A small increase in ventricular fluid pressure (1.3 +/- 0.3 mm Hg) occurs on isovolumetic addition of meglumine iothalamate to the lateral ventricle. A marked increase (46.3 +/- 3.2 mm Hg: P less than 0.001) in pressure occurs on passage of the contrast medium into the posterior part of the third ventricle; the aqueduct and the fourth ventricle. Theoretical mechanisms are discussed.", "contents": "Intracranial pressure during ventriculography using meglumine iothalamate. A small increase in ventricular fluid pressure (1.3 +/- 0.3 mm Hg) occurs on isovolumetic addition of meglumine iothalamate to the lateral ventricle. A marked increase (46.3 +/- 3.2 mm Hg: P less than 0.001) in pressure occurs on passage of the contrast medium into the posterior part of the third ventricle; the aqueduct and the fourth ventricle. Theoretical mechanisms are discussed."}
{"id": "PMID:1085559", "title": "The structure of the rhombencephalic roof in the frog.", "content": "The structure of the rhombencephalic roof has been studied by serial section histology in 12 adult frogs, 3 recently metamorphosed frogs and 8 tadpoles of the species Rana temporaria. The roof of the rhombencephalon caudal to the choroid plexus consists of a delicate layer of ependyma which appears to be intact from the time of its development in the tadpole until the stage of metamorphosis. In the adult frog it is an attenuated structure displaying variable deficiencies with an apparent communication between the rhombencephalic ventricle and the surrounding subarachnoid space. The significance of this acquired communication between internal and external cerebrospinal fluid compartments in the adult frog is discussed in relationship to the classical concepts of bulk flow of the CSF in humans. Some of the limitations of these classical concepts are also discussed, and the approach to these problems by studying the comparative morphology of the CSF system in vertebrate animals is justified.", "contents": "The structure of the rhombencephalic roof in the frog. The structure of the rhombencephalic roof has been studied by serial section histology in 12 adult frogs, 3 recently metamorphosed frogs and 8 tadpoles of the species Rana temporaria. The roof of the rhombencephalon caudal to the choroid plexus consists of a delicate layer of ependyma which appears to be intact from the time of its development in the tadpole until the stage of metamorphosis. In the adult frog it is an attenuated structure displaying variable deficiencies with an apparent communication between the rhombencephalic ventricle and the surrounding subarachnoid space. The significance of this acquired communication between internal and external cerebrospinal fluid compartments in the adult frog is discussed in relationship to the classical concepts of bulk flow of the CSF in humans. Some of the limitations of these classical concepts are also discussed, and the approach to these problems by studying the comparative morphology of the CSF system in vertebrate animals is justified."}
{"id": "PMID:1085561", "title": "Angioimmunoblastic lymphadenopathy with dysproteinemia. Immunohistologic and ultrastructural studies.", "content": "Immunoblasts of the B-cell line and immunoblasts of the T-cell line have very similar light-microscopic appearances but different immunologic compositions. The immunoblasts of a patient with angioimmunoblastic lymphadenopathy with dysproteinemia were found to bear surface immunoglobulin and complement receptor. They lacked receptor for cytophilic antibody. Surface villous projections were demonstrated by scanning electron microscopy. These features strongly indicate their B-cell origin.", "contents": "Angioimmunoblastic lymphadenopathy with dysproteinemia. Immunohistologic and ultrastructural studies. Immunoblasts of the B-cell line and immunoblasts of the T-cell line have very similar light-microscopic appearances but different immunologic compositions. The immunoblasts of a patient with angioimmunoblastic lymphadenopathy with dysproteinemia were found to bear surface immunoglobulin and complement receptor. They lacked receptor for cytophilic antibody. Surface villous projections were demonstrated by scanning electron microscopy. These features strongly indicate their B-cell origin."}
{"id": "PMID:1085563", "title": "Portal hypertension. A new beginning for an old problem.", "content": "There are two types of shunts for the treatment of esophageal varices: total shunts or selective shunts. The various total shunts are designed to \"decompress\" both the portal and the variceal venous systems. In contrast, a selective shunt decompresses the varices only, and the portal pressure and presumably portal flow are not affected. The very purpose of the total shunt is the total or partial diversion of portal flow. This is the case no matter which branches of the portal and systemic venous systems are connected and whether the anastomosis is a direct one or is through a graft. In an ongoing prospective, randomized clinical trial, which now includes 46 patients, a total shunt (mesorenal H graft) and the selective distal splenorenal (Warren) shunt are compared. The operative mortality rate and the frequency of postoperative bleeding are the same. However, encephalopathy is more frequent after the total shunt (P less than 0.05), and a trend is developing which suggests a better long-term survival rate after the Warren shunt. If these trends continue, then the superiority of the Warren shunt is established in a selected group of patients who have bled at least once from their varices. If these patients continue to remain free of encephalopathy and hemorrhage will not recur, then it is probable that the Warren shunt could improve the chances of survival of cirrhotic patients with esophageal varices who did not bleed yet. The \"prophylactic\" value of the Warren shunt will require a multicenter, cooperative, controlled clinical trial.", "contents": "Portal hypertension. A new beginning for an old problem. There are two types of shunts for the treatment of esophageal varices: total shunts or selective shunts. The various total shunts are designed to \"decompress\" both the portal and the variceal venous systems. In contrast, a selective shunt decompresses the varices only, and the portal pressure and presumably portal flow are not affected. The very purpose of the total shunt is the total or partial diversion of portal flow. This is the case no matter which branches of the portal and systemic venous systems are connected and whether the anastomosis is a direct one or is through a graft. In an ongoing prospective, randomized clinical trial, which now includes 46 patients, a total shunt (mesorenal H graft) and the selective distal splenorenal (Warren) shunt are compared. The operative mortality rate and the frequency of postoperative bleeding are the same. However, encephalopathy is more frequent after the total shunt (P less than 0.05), and a trend is developing which suggests a better long-term survival rate after the Warren shunt. If these trends continue, then the superiority of the Warren shunt is established in a selected group of patients who have bled at least once from their varices. If these patients continue to remain free of encephalopathy and hemorrhage will not recur, then it is probable that the Warren shunt could improve the chances of survival of cirrhotic patients with esophageal varices who did not bleed yet. The \"prophylactic\" value of the Warren shunt will require a multicenter, cooperative, controlled clinical trial."}
{"id": "PMID:1085565", "title": "Diagnostic accuracy of fiberoptic panendoscopy and visceral angiography in acute upper gastrointestinal bleeding.", "content": "Emergency fiberoptic panendoscopy and visceral angiography both had comparable diagnostic accuracy in our series of 55 patients with actively bleeding upper gastrointestinal lesions. The diagnostic accuracy of the barium meal was found inferior to both fiberoptic panendoscopy and visceral angiography. Panendoscopy proved capable of quickly and safely diagnosing site and source of the active bleeding lesion. Visceral angiography requiring additional time, expense and personnel commitment proved an effective back-up procedure when panendoscopy was unsuccessful or contradictions existed. Emergency angiography was well tolerated by gravely ill patients. The therapeutic advantage of angiography with infusion of vasopressin upon completion of the diagnostic study remains to be shown as an advantage over panendoscopy.", "contents": "Diagnostic accuracy of fiberoptic panendoscopy and visceral angiography in acute upper gastrointestinal bleeding. Emergency fiberoptic panendoscopy and visceral angiography both had comparable diagnostic accuracy in our series of 55 patients with actively bleeding upper gastrointestinal lesions. The diagnostic accuracy of the barium meal was found inferior to both fiberoptic panendoscopy and visceral angiography. Panendoscopy proved capable of quickly and safely diagnosing site and source of the active bleeding lesion. Visceral angiography requiring additional time, expense and personnel commitment proved an effective back-up procedure when panendoscopy was unsuccessful or contradictions existed. Emergency angiography was well tolerated by gravely ill patients. The therapeutic advantage of angiography with infusion of vasopressin upon completion of the diagnostic study remains to be shown as an advantage over panendoscopy."}
{"id": "PMID:1085566", "title": "Tritanopia.", "content": "The color-matching functions of individual tritanopes differ significantly from those predicted with the assumption that the two cone pigments of the tritanope are those which underlie the matches of the standard observer. The differences cannot be explained completely by the abnormal luminosity curve of the standard observer nor by abnormalities in transmissivity of the eye media. Individual tritanopes differ significantly in their color matches in ways not entirely accounted for by eye media differences. The results are consistent with the view that there are a variety of different long- and medium-wave-sensitive cone visual pigments among different tritanopes.", "contents": "Tritanopia. The color-matching functions of individual tritanopes differ significantly from those predicted with the assumption that the two cone pigments of the tritanope are those which underlie the matches of the standard observer. The differences cannot be explained completely by the abnormal luminosity curve of the standard observer nor by abnormalities in transmissivity of the eye media. Individual tritanopes differ significantly in their color matches in ways not entirely accounted for by eye media differences. The results are consistent with the view that there are a variety of different long- and medium-wave-sensitive cone visual pigments among different tritanopes."}
{"id": "PMID:1085569", "title": "Mitochondria-rich cells of frog skin in transport mechanisms: morphological and kinetic studies on transepithelial excretion of methylene blue.", "content": "The following study combines histological observations on the localization and density of mitochondria-rich cells with a kinetic study of the part played by these cells in transport phenomena. In the ventral skin, mitochondria-rich cells and granular cells are equally abundant in the first living cell layer. The mitochondria-rich cells were shown to excrete methylene blue [3,9-bis-(dimethylamino)-phenozathionum chloride]. A kinetic study of this excretion across the isolated epithelium of Rana esculenta skin showed that the mechanism of the excretion was a saturable one. An important fraction of methylene blue excretion depends on the absorption of sodium; the absence of this ion, or the inhibition of its transport by ouabain or amiloride, inhibits the excretion of the organic base. The mitochondria-rich cells, however, do not appear to play a determining role in sodium transport.", "contents": "Mitochondria-rich cells of frog skin in transport mechanisms: morphological and kinetic studies on transepithelial excretion of methylene blue. The following study combines histological observations on the localization and density of mitochondria-rich cells with a kinetic study of the part played by these cells in transport phenomena. In the ventral skin, mitochondria-rich cells and granular cells are equally abundant in the first living cell layer. The mitochondria-rich cells were shown to excrete methylene blue [3,9-bis-(dimethylamino)-phenozathionum chloride]. A kinetic study of this excretion across the isolated epithelium of Rana esculenta skin showed that the mechanism of the excretion was a saturable one. An important fraction of methylene blue excretion depends on the absorption of sodium; the absence of this ion, or the inhibition of its transport by ouabain or amiloride, inhibits the excretion of the organic base. The mitochondria-rich cells, however, do not appear to play a determining role in sodium transport."}
{"id": "PMID:1085570", "title": "Lactate and contractile force in frog muscle during development of fatigue and recovery.", "content": "The relationship between lactic acid concentration and twitch tension was reevaluated in electrically stimulated frog sartorius muscle. In muscles stimulated under anaerobic conditions at a rate of 30 stimuli/MIN CONTRACTILE FORCE DECREASED TO 36% OF THE INITIAL VALUE IN 15 MIN, Concomitantly lactate increased from 3.3 to 18.7 mumol/g of muscle. The correlaiton between the increase in lactate and the decrease in contractile force was significant (r = -0.99, P less than 0.000001). Recovery occurred in two phases. A rapid increase in contractile force, which represented 20% of the total recovery, took place during the first 15 s and occurred concomitantly with an increase in ATP from 3.9 to 4.6 mumol/g. Lactate concentration did not change significantly during this period. The second phase of recovery of contractile force was complete in 50 min. Lactate concentration and contractile force were significatly correlated during recovery (r = -0;92, P less than 0.00001). However, recovery of contractile force lagged behind the decrease in lactate; a given concentration of muscle lactate was associated with a higher contractile force early during development of fatigue than late during recovery.", "contents": "Lactate and contractile force in frog muscle during development of fatigue and recovery. The relationship between lactic acid concentration and twitch tension was reevaluated in electrically stimulated frog sartorius muscle. In muscles stimulated under anaerobic conditions at a rate of 30 stimuli/MIN CONTRACTILE FORCE DECREASED TO 36% OF THE INITIAL VALUE IN 15 MIN, Concomitantly lactate increased from 3.3 to 18.7 mumol/g of muscle. The correlaiton between the increase in lactate and the decrease in contractile force was significant (r = -0.99, P less than 0.000001). Recovery occurred in two phases. A rapid increase in contractile force, which represented 20% of the total recovery, took place during the first 15 s and occurred concomitantly with an increase in ATP from 3.9 to 4.6 mumol/g. Lactate concentration did not change significantly during this period. The second phase of recovery of contractile force was complete in 50 min. Lactate concentration and contractile force were significatly correlated during recovery (r = -0;92, P less than 0.00001). However, recovery of contractile force lagged behind the decrease in lactate; a given concentration of muscle lactate was associated with a higher contractile force early during development of fatigue than late during recovery."}
{"id": "PMID:1085571", "title": "Gastric H+ secretion in presence of substrates: absolute dependence on secretagogues.", "content": "Spontaneously secreting gastric mucosae of the bullfrog (Rana catesbeiana) were brought to resting states in the absence of both the exogenous secretagogue and energy-yielding substrate, either by treatment with burimamide or by the classical method of prolonged preincubation. Glucose, pyruvate, lactate, and tricarboxylic acid cycle intermediates were compared with short- and long-chain fatty acids as potential substrates on these resting mucosae to determine if the latter could initiate and sustain H+ secretion in the absence of added secretagogues. The experimental data indicate that fatty acids cannot stimulate acid secretion from the resting state by preempting the requirement for secretagogues. Although fatty acids may serve as preferred substrates in comparison with glucose, glycolytic intermediates, and tricarboxylic acid cycle intermediates, acid secretion is obligatorily dependent on the presence of endogenous or added secretagogues. The implications of this finding with respect to one of the recently proposed hypothesis for secretagogue regulation of gastric hydrochloric acid secretion are discussed.", "contents": "Gastric H+ secretion in presence of substrates: absolute dependence on secretagogues. Spontaneously secreting gastric mucosae of the bullfrog (Rana catesbeiana) were brought to resting states in the absence of both the exogenous secretagogue and energy-yielding substrate, either by treatment with burimamide or by the classical method of prolonged preincubation. Glucose, pyruvate, lactate, and tricarboxylic acid cycle intermediates were compared with short- and long-chain fatty acids as potential substrates on these resting mucosae to determine if the latter could initiate and sustain H+ secretion in the absence of added secretagogues. The experimental data indicate that fatty acids cannot stimulate acid secretion from the resting state by preempting the requirement for secretagogues. Although fatty acids may serve as preferred substrates in comparison with glucose, glycolytic intermediates, and tricarboxylic acid cycle intermediates, acid secretion is obligatorily dependent on the presence of endogenous or added secretagogues. The implications of this finding with respect to one of the recently proposed hypothesis for secretagogue regulation of gastric hydrochloric acid secretion are discussed."}
{"id": "PMID:1085572", "title": "Effect of muscle length on 45Ca efflux in resting and contracting skeletal muscle.", "content": "The increment of the slow component of 45Ca efflux from frog skeletal muscle that is produced by electrical stimulation is reduced by 70% when the muscle has been stretched to 160% of in vivo length. No change in the efflux from resting muscles or the increment in influx associated with stimulation was produced by increased length. The increased efflux produced by 8 mM caffeine was not altered by increased muscle length. Resting and action potentials were unchanged by the stretching. No morphological changes were seen in the transverse tubules or their junction with the terminal cisternae even at sarcomere lengths of 3.8 muM, but the terminal cisternae themselves were elongated at this length.", "contents": "Effect of muscle length on 45Ca efflux in resting and contracting skeletal muscle. The increment of the slow component of 45Ca efflux from frog skeletal muscle that is produced by electrical stimulation is reduced by 70% when the muscle has been stretched to 160% of in vivo length. No change in the efflux from resting muscles or the increment in influx associated with stimulation was produced by increased length. The increased efflux produced by 8 mM caffeine was not altered by increased muscle length. Resting and action potentials were unchanged by the stretching. No morphological changes were seen in the transverse tubules or their junction with the terminal cisternae even at sarcomere lengths of 3.8 muM, but the terminal cisternae themselves were elongated at this length."}
{"id": "PMID:1085573", "title": "Postemetic hematomas: a variant of the Mallory-Weiss syndrome.", "content": "Of forty-four patients with the Mallory-Weiss syndrome, nine (20 per cent) had endoscopic evidence of small submucosal hematoma(s) lying in or adjacent to the region of the the tear. These small hematomas are believed to occur most commonly as a variant of the Mallory-Weiss lesion.", "contents": "Postemetic hematomas: a variant of the Mallory-Weiss syndrome. Of forty-four patients with the Mallory-Weiss syndrome, nine (20 per cent) had endoscopic evidence of small submucosal hematoma(s) lying in or adjacent to the region of the the tear. These small hematomas are believed to occur most commonly as a variant of the Mallory-Weiss lesion."}
{"id": "PMID:1085574", "title": "A retroperitoneal cystic leiomyosarcoma spontaneously draining into the stomach.", "content": "An unusual case of a retroperitoneal cystic leiomyosarcoma clinically resembling a pancreatic pseudocyst with subsequent spontaneous cystgastrostomy drainage is presented.", "contents": "A retroperitoneal cystic leiomyosarcoma spontaneously draining into the stomach. An unusual case of a retroperitoneal cystic leiomyosarcoma clinically resembling a pancreatic pseudocyst with subsequent spontaneous cystgastrostomy drainage is presented."}
{"id": "PMID:1085577", "title": "Lysosomal enzymes in subretinal fluid.", "content": "Samples of subretinal fluid (SRF) from patients with primary rhegmatogeneous retinal detachment of various duration were studied using standard enzymologic techniques. The protein content in SRF increased with the duration of detachment. The activities of lysosomal enzymes in SRF, which were acid phosphatase, beta-glucuronidase, and cathepsin D, also increased with the duration of detachment. Especially cathepsin D, which was not detected in serum, was present in SRF. The activity of a nonlysosomal enzyme, i. e., lactic dehydrogenase, which was used as a marker of cell disruption and of serum transudation was the same as that of serum. Because lysosomal enzymes are known to be able to degrade cells and tissues, the enzymologic analysis of SRF contributes to our understanding of the pathology of primary rhegmatogeneous retinal detachment.", "contents": "Lysosomal enzymes in subretinal fluid. Samples of subretinal fluid (SRF) from patients with primary rhegmatogeneous retinal detachment of various duration were studied using standard enzymologic techniques. The protein content in SRF increased with the duration of detachment. The activities of lysosomal enzymes in SRF, which were acid phosphatase, beta-glucuronidase, and cathepsin D, also increased with the duration of detachment. Especially cathepsin D, which was not detected in serum, was present in SRF. The activity of a nonlysosomal enzyme, i. e., lactic dehydrogenase, which was used as a marker of cell disruption and of serum transudation was the same as that of serum. Because lysosomal enzymes are known to be able to degrade cells and tissues, the enzymologic analysis of SRF contributes to our understanding of the pathology of primary rhegmatogeneous retinal detachment."}
{"id": "PMID:1085578", "title": "[Possibilities of cartographically documenting changes of the vitreous body, when examining the eye with a three-mirror contact glass (author's transl)].", "content": "After some remarks about the technique of the examination of the vitreous body with the three-mirror contact lens, the usual charts for documentation of vitreous changes are described. Furthermore, a new method of direct diagrammatic documentation of biomicroscopic findings of vitreous changes is presented.", "contents": "[Possibilities of cartographically documenting changes of the vitreous body, when examining the eye with a three-mirror contact glass (author's transl)]. After some remarks about the technique of the examination of the vitreous body with the three-mirror contact lens, the usual charts for documentation of vitreous changes are described. Furthermore, a new method of direct diagrammatic documentation of biomicroscopic findings of vitreous changes is presented."}
{"id": "PMID:1085579", "title": "Enzymologic and histologic investigations in normal and pressure-ischemic retina of rabbits.", "content": "Histologic investigations together with histochemical and photometric measurements of enzyme activities were performed in retina of rabbits, whose blood supply had been totally interrupted for 1h. A retinal edema developed affecting the internal layers between the inner limiting membrane and the internal plexiform and ganglion cell layer. Although this edema was quite remarkable at the posterior pole of the eye, it diminished toward the periphery, disappearing near the ora serrata. The activities of the following enzymes were investigated: hexokinase, glucose 6-phosphate dehydrogenase, aldolase, glyceraldehydephosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, ATPase, and phosphorylase. The most striking finding was the total disappearance of phosphorylase activity under pressure ischemia. ATPase and aldolase showed a decreased activity in the ischemic retina, and malate dehydrogenase a slightly diminished one. Concerning the other enzymes, no significant differences between normal and ischemic retina were observed.", "contents": "Enzymologic and histologic investigations in normal and pressure-ischemic retina of rabbits. Histologic investigations together with histochemical and photometric measurements of enzyme activities were performed in retina of rabbits, whose blood supply had been totally interrupted for 1h. A retinal edema developed affecting the internal layers between the inner limiting membrane and the internal plexiform and ganglion cell layer. Although this edema was quite remarkable at the posterior pole of the eye, it diminished toward the periphery, disappearing near the ora serrata. The activities of the following enzymes were investigated: hexokinase, glucose 6-phosphate dehydrogenase, aldolase, glyceraldehydephosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, ATPase, and phosphorylase. The most striking finding was the total disappearance of phosphorylase activity under pressure ischemia. ATPase and aldolase showed a decreased activity in the ischemic retina, and malate dehydrogenase a slightly diminished one. Concerning the other enzymes, no significant differences between normal and ischemic retina were observed."}
{"id": "PMID:1085580", "title": "The role of lactic acid in retinal neovascularization.", "content": "In order to gain information on the possible role of lactate in the development of neovascularization following retinal malperfusion, the lactate content in vitreous-retina-choroid specimens of 02-treated kittens and streptozotocin-diabetic rats was determined. In both experiments there was no significant difference of lactate content between the diseased eyes and healthy controls.", "contents": "The role of lactic acid in retinal neovascularization. In order to gain information on the possible role of lactate in the development of neovascularization following retinal malperfusion, the lactate content in vitreous-retina-choroid specimens of 02-treated kittens and streptozotocin-diabetic rats was determined. In both experiments there was no significant difference of lactate content between the diseased eyes and healthy controls."}
{"id": "PMID:1085581", "title": "[Stereoscopic vision with alternating presentation of stereo pairs (author's transl)].", "content": "The threshold values for stereopsis with alternating monocular presentation of the corresponding member of a stereo pair was examined in relationship to the stimulus duration and the interocular delay. The maximum interocular delay for short stimulus durations was approximately 190 msec. The maximum stimulus duration was between 400-500 msec. At this stimulus duration the interocular delay must approach zero if stereopsis is to be maintained. In the threshold region the stereo effect diminished. One can possibly explain this phenomenon as a partial sensorial fusion. For normal appreciation of stereopsis, the critical frequency was about 2 Hz over the threshold values found with a given stimulus duration.", "contents": "[Stereoscopic vision with alternating presentation of stereo pairs (author's transl)]. The threshold values for stereopsis with alternating monocular presentation of the corresponding member of a stereo pair was examined in relationship to the stimulus duration and the interocular delay. The maximum interocular delay for short stimulus durations was approximately 190 msec. The maximum stimulus duration was between 400-500 msec. At this stimulus duration the interocular delay must approach zero if stereopsis is to be maintained. In the threshold region the stereo effect diminished. One can possibly explain this phenomenon as a partial sensorial fusion. For normal appreciation of stereopsis, the critical frequency was about 2 Hz over the threshold values found with a given stimulus duration."}
{"id": "PMID:1085582", "title": "[On aplasia of the optic nerve (author's transl)].", "content": "The occurrence of aplasia and hypoplasia of the optic nerve in man and in mammals is described. A secondary aplasia may result from additional malformations of the central nervous system. Primary aplasia may be studied in mutants of the house mouse, which show that not only the blood vessels, but also changes in time and location of the physiologic cell necrosis may be important.", "contents": "[On aplasia of the optic nerve (author's transl)]. The occurrence of aplasia and hypoplasia of the optic nerve in man and in mammals is described. A secondary aplasia may result from additional malformations of the central nervous system. Primary aplasia may be studied in mutants of the house mouse, which show that not only the blood vessels, but also changes in time and location of the physiologic cell necrosis may be important."}
{"id": "PMID:1085585", "title": "[Thyrotropia].", "content": "The significance of the radioimmunological dosage of the thyrotropic hormone (TSH) is examined as regards accordance between the experimental measure and the real behaviour of the hormone; as a matter of fact, in some particular conditions the problem of the specificity of dosage must be taken into account because of the possible interference of other hormones. From the clinical point of view the TSH radioimmuno assay is advisable for its accuracy, sensibility and specificity. On the other other hand, it is a lengthy and complex determination; instability of the labelled hormone is to be pointed out as the worst shortcoming. The procedures for clinical study of TSH are also examined with reference to an indirect evaluation of the thyroid function. tthe clinical application of TSH dosage in endrocrinology diagnostics, concerning both thyroid and hypophysis pathology, are discussed.", "contents": "[Thyrotropia]. The significance of the radioimmunological dosage of the thyrotropic hormone (TSH) is examined as regards accordance between the experimental measure and the real behaviour of the hormone; as a matter of fact, in some particular conditions the problem of the specificity of dosage must be taken into account because of the possible interference of other hormones. From the clinical point of view the TSH radioimmuno assay is advisable for its accuracy, sensibility and specificity. On the other other hand, it is a lengthy and complex determination; instability of the labelled hormone is to be pointed out as the worst shortcoming. The procedures for clinical study of TSH are also examined with reference to an indirect evaluation of the thyroid function. tthe clinical application of TSH dosage in endrocrinology diagnostics, concerning both thyroid and hypophysis pathology, are discussed."}
{"id": "PMID:1085587", "title": "Experimental autoimmune myasthenia gravis: cellular and humoral immune responses.", "content": "Rats inoculated intradermally with eel acetylcholine receptor protein(AChR) with adjuvants developed autoimmunity to skeletal muscle AChR. This is evidenced clinically as two episodes of experimental autoimmune myasthenia gravis (EAMG), an acute phase that occurs early (8 days) and is transient, and a chronic phase (30 days) that is usually progressive. Positive delayed cutaneous reactivity appeared at day 4 and serum antibody to eel AChR was detectable by day 7 postinoculation. After day 25 the titer of antibody to syngeneic muscle AChR  rose abruptly. Antibody to muscle AChR sedimented as 7S.  Lymph node cells from rats sensitized to AChR  were capable of transferring EAMG to normal recipients. Thymectomy after the onset of EAMG  had no effect. Early treatment in vivo with antithymocyte serum suppressed acute  but not chronic phase EAMG. Experiments combining thymectomy, x-irradiation and reconstitution with distinct populations of lymphocytes indicated athat thymus-derived lymphocytes are required for induction of EAMG  and antibody to AChR. These data suggest that both cellular and humoral responses to AChR, either sequentially or in combination, contribute to the pathogenesis of EAMG.", "contents": "Experimental autoimmune myasthenia gravis: cellular and humoral immune responses. Rats inoculated intradermally with eel acetylcholine receptor protein(AChR) with adjuvants developed autoimmunity to skeletal muscle AChR. This is evidenced clinically as two episodes of experimental autoimmune myasthenia gravis (EAMG), an acute phase that occurs early (8 days) and is transient, and a chronic phase (30 days) that is usually progressive. Positive delayed cutaneous reactivity appeared at day 4 and serum antibody to eel AChR was detectable by day 7 postinoculation. After day 25 the titer of antibody to syngeneic muscle AChR  rose abruptly. Antibody to muscle AChR sedimented as 7S.  Lymph node cells from rats sensitized to AChR  were capable of transferring EAMG to normal recipients. Thymectomy after the onset of EAMG  had no effect. Early treatment in vivo with antithymocyte serum suppressed acute  but not chronic phase EAMG. Experiments combining thymectomy, x-irradiation and reconstitution with distinct populations of lymphocytes indicated athat thymus-derived lymphocytes are required for induction of EAMG  and antibody to AChR. These data suggest that both cellular and humoral responses to AChR, either sequentially or in combination, contribute to the pathogenesis of EAMG."}
{"id": "PMID:1085589", "title": "HL-A antigens, autoantibody production, and associated diseases in thymoma patients, with and without myasthenia gravis.", "content": "The sera of 67 patients with thymomas-43 with myasthenia gravis (thymoma(+) MG(+)) and 24 without myasthenia gravis (thymoma (+) MG (-)) from 5 Dutch centers were examined with the indirect immunofluorescence test for the presence of antibodies reacting with skeletal muscle (AMA), thyroid tissue, gastric parietal cells, adrenocortex and antinuclear antibodies (ANA). The data were compared with those obtained in a group 83 MG patients in whom a thymoma was excluded by histological verification (thymoma (-) MG (+)) and with 1106 controls from the normal population. Histocompatibility (HL-A) antigens were tested in 24 thymoma (+) MG (+), 23  thymoma (+) MG (-), and 43 thymoma (-) MG (+) patients and 533 controls from the Dutch population. AMA was found in all thymoma (+) MG (+) cases, in 42% of thymoma (+) MG (-), in 11% of thymoma (-) MG (+), and in less than 1% of the controls. The differences between each of these groups are significant (p less than 0.01, Table 3.) ANA was found in 54%, 50%, 18%, and 4%, respectively, of the above mentioned groups. The differences between the thymoma (+) and the thymoma (-) groups are significant (p less than 0.001. Table 4). The frequencies of antibodies reacting with thyroid tissue, gastric parietal cells and adrenocortex were low and showed no differences between the groups. The frequencies of HL-A8 were significantly decreased in both thymoma groups that had a relatively high incidence of associated immunological disorders (Table 5). Patients with MG and without antimuscle antibodies have no thymomas: This rule is of practical value in the management of the MG patient.", "contents": "HL-A antigens, autoantibody production, and associated diseases in thymoma patients, with and without myasthenia gravis. The sera of 67 patients with thymomas-43 with myasthenia gravis (thymoma(+) MG(+)) and 24 without myasthenia gravis (thymoma (+) MG (-)) from 5 Dutch centers were examined with the indirect immunofluorescence test for the presence of antibodies reacting with skeletal muscle (AMA), thyroid tissue, gastric parietal cells, adrenocortex and antinuclear antibodies (ANA). The data were compared with those obtained in a group 83 MG patients in whom a thymoma was excluded by histological verification (thymoma (-) MG (+)) and with 1106 controls from the normal population. Histocompatibility (HL-A) antigens were tested in 24 thymoma (+) MG (+), 23  thymoma (+) MG (-), and 43 thymoma (-) MG (+) patients and 533 controls from the Dutch population. AMA was found in all thymoma (+) MG (+) cases, in 42% of thymoma (+) MG (-), in 11% of thymoma (-) MG (+), and in less than 1% of the controls. The differences between each of these groups are significant (p less than 0.01, Table 3.) ANA was found in 54%, 50%, 18%, and 4%, respectively, of the above mentioned groups. The differences between the thymoma (+) and the thymoma (-) groups are significant (p less than 0.001. Table 4). The frequencies of antibodies reacting with thyroid tissue, gastric parietal cells and adrenocortex were low and showed no differences between the groups. The frequencies of HL-A8 were significantly decreased in both thymoma groups that had a relatively high incidence of associated immunological disorders (Table 5). Patients with MG and without antimuscle antibodies have no thymomas: This rule is of practical value in the management of the MG patient."}
{"id": "PMID:1085590", "title": "Characterization of peripheral blood lymphocytes in sarcoidosis.", "content": "In the basal state, patients with sarcoidosis have reduced numbers of circulating T lymphocytes and a normal number of circulating B lymphocytes. The lymphocytes show impaired DNA synthesis in response to several mitogens and antigens and impaired K-cell cytotoxicity. Circulating atypical cells of lymphocytic origin are present belonging to both T- and B-cell lineages. After work (with mobilization of lymphocytes) the T-cell deficiency is further accentuated compared with normals. Moreover, after work a deficiency in the number of circulating B cells is also encountered. The atypical cells do not increase. Compared with normals the impaired K-cell cytotoxicity and lower levels of DNA synthesis also persist. It is suggested that the deviations found in sarcoidosis are consequences of a disease process affecting the lymphoid tissues and successively replacing the normal structures. The presence in vivo of DNA-synthesizing circulating lymphocytes may indicate a persistent antigenic stimulation.", "contents": "Characterization of peripheral blood lymphocytes in sarcoidosis. In the basal state, patients with sarcoidosis have reduced numbers of circulating T lymphocytes and a normal number of circulating B lymphocytes. The lymphocytes show impaired DNA synthesis in response to several mitogens and antigens and impaired K-cell cytotoxicity. Circulating atypical cells of lymphocytic origin are present belonging to both T- and B-cell lineages. After work (with mobilization of lymphocytes) the T-cell deficiency is further accentuated compared with normals. Moreover, after work a deficiency in the number of circulating B cells is also encountered. The atypical cells do not increase. Compared with normals the impaired K-cell cytotoxicity and lower levels of DNA synthesis also persist. It is suggested that the deviations found in sarcoidosis are consequences of a disease process affecting the lymphoid tissues and successively replacing the normal structures. The presence in vivo of DNA-synthesizing circulating lymphocytes may indicate a persistent antigenic stimulation."}
{"id": "PMID:1085591", "title": "A preliminary report on a study of serum alpha-1-antitrypsin and immunoglobulin levels in lepromatous leprosy.", "content": "IgG, IgA, and IgM immunoglobulins and alpha-1-antitrypsin globulin in sera from 30 patients with lepromatous leprosy were determined and compared with 30 age- and sex-matched healthy controls. Mean levels of IgG, IgM, IgA, and alpha-1-antitrypsin were significantly elevated in the sera of lepromatous leprosy patients in comparison with levels found in normal subjects. Statistical analysis of the relationships between levels of alpha-1-antitrypsin and immunoglobulins in the sera of lepromatous leprosy patients and controls revealed no correlation. It is therefore concluded that factors responsible for concentrations of immunoglobulins and alpha-1-antitrypsin are independent of each other in patients with lepromatous leprosy.", "contents": "A preliminary report on a study of serum alpha-1-antitrypsin and immunoglobulin levels in lepromatous leprosy. IgG, IgA, and IgM immunoglobulins and alpha-1-antitrypsin globulin in sera from 30 patients with lepromatous leprosy were determined and compared with 30 age- and sex-matched healthy controls. Mean levels of IgG, IgM, IgA, and alpha-1-antitrypsin were significantly elevated in the sera of lepromatous leprosy patients in comparison with levels found in normal subjects. Statistical analysis of the relationships between levels of alpha-1-antitrypsin and immunoglobulins in the sera of lepromatous leprosy patients and controls revealed no correlation. It is therefore concluded that factors responsible for concentrations of immunoglobulins and alpha-1-antitrypsin are independent of each other in patients with lepromatous leprosy."}
{"id": "PMID:1085592", "title": "Immunological identification of subpopulations of mononuclear cells in sarcoid granulomas.", "content": "The quantity of both circulating B and T lymphocytes in patients with sarcoidosis is diminished, although the relative percentage of each cell type appears normal. Immunocompetent cells comprising B and T lymphocytes, plasma cells, and histiocytes have been recognized in the sarcoid granuloma and the implications of these findings have been discussed.", "contents": "Immunological identification of subpopulations of mononuclear cells in sarcoid granulomas. The quantity of both circulating B and T lymphocytes in patients with sarcoidosis is diminished, although the relative percentage of each cell type appears normal. Immunocompetent cells comprising B and T lymphocytes, plasma cells, and histiocytes have been recognized in the sarcoid granuloma and the implications of these findings have been discussed."}
{"id": "PMID:1085593", "title": "Immunological studies in sarcoidosis: a comparison of disease activity and various immunological parameters.", "content": "We found it valuable to separate the heterogeneous types of sarcoidosis into more homogeneous groups on the basis of activity and duration of the disease. This view is supported in the present study by the finding of a marked depression of T-cell function in patients with chronic-active sarcoidosis. Patients with acute or chronic-inactive disease had only moderately depressed T-cell function as measured by tuberculin skin test and DNCB index. These results are in agreement with those of some previous investigations. The remainder of the abnormal findings, particularly low total number of circulation T lymphocytes, elevated serum IgG levels, and presence of autoantibodies, could not be correlated to disease activity, extent of the disease, or T-cell function. We have found no explanation for the presence of autoantibodies but suspect that they may be nonspecifically related to the disease process.", "contents": "Immunological studies in sarcoidosis: a comparison of disease activity and various immunological parameters. We found it valuable to separate the heterogeneous types of sarcoidosis into more homogeneous groups on the basis of activity and duration of the disease. This view is supported in the present study by the finding of a marked depression of T-cell function in patients with chronic-active sarcoidosis. Patients with acute or chronic-inactive disease had only moderately depressed T-cell function as measured by tuberculin skin test and DNCB index. These results are in agreement with those of some previous investigations. The remainder of the abnormal findings, particularly low total number of circulation T lymphocytes, elevated serum IgG levels, and presence of autoantibodies, could not be correlated to disease activity, extent of the disease, or T-cell function. We have found no explanation for the presence of autoantibodies but suspect that they may be nonspecifically related to the disease process."}
{"id": "PMID:1085597", "title": "Intravitreal erythromycin in the treatment of induced staphylococcal endophthalmitis.", "content": "Intravitreal injection of 500 mcg of erythromycin as the gluceptate caused no toxicity to ocular structures. This dose maintained bacterial inhibitory concentrations in the vitreous for up to 24 hours. Consistent aqueous levels were not obtained with intravitreal injection. Intravitreal treatment of Staphylococcus aureus endophthalmitis was successful in 11 of 13 rabbit eyes compared to 2 of 13 treated with subconjunctival erythromycin.", "contents": "Intravitreal erythromycin in the treatment of induced staphylococcal endophthalmitis. Intravitreal injection of 500 mcg of erythromycin as the gluceptate caused no toxicity to ocular structures. This dose maintained bacterial inhibitory concentrations in the vitreous for up to 24 hours. Consistent aqueous levels were not obtained with intravitreal injection. Intravitreal treatment of Staphylococcus aureus endophthalmitis was successful in 11 of 13 rabbit eyes compared to 2 of 13 treated with subconjunctival erythromycin."}
{"id": "PMID:1085598", "title": "Surgical management of ventricular septal defect complicating myocardial infarction.", "content": "In an 18 month period six patients were treated by operation for ventricular septal defect occurring as a complication of acute myocardial infarction. Each septal defect occurred within one week of the myocardial infarct but the interval from infarction to operation ranged from 8 days to 7 months. All patients had intractable cardiac failure and two were in cardiogenic shock at the time of operation. Preoperatively right and left heart catheterisation with left ventriculography and biplane coronary arteriography was performed in every case. At operation the principle of \"total correction\" of all the cardiac defects was followed so that in addition to closure of the septal defect each patient required one or more additional operative procedures such as resection of left ventricular infarct or aneurysm, mitral valve replacement or coronary artery vein bypass grafts. All six patients survived operation but one died four weeks postoperatively from perforation of an acute peptic ulcer. In one patient the ventricular septal defect recurred and was successfully closed four months later. The surviving patients remain well at follow-up.", "contents": "Surgical management of ventricular septal defect complicating myocardial infarction. In an 18 month period six patients were treated by operation for ventricular septal defect occurring as a complication of acute myocardial infarction. Each septal defect occurred within one week of the myocardial infarct but the interval from infarction to operation ranged from 8 days to 7 months. All patients had intractable cardiac failure and two were in cardiogenic shock at the time of operation. Preoperatively right and left heart catheterisation with left ventriculography and biplane coronary arteriography was performed in every case. At operation the principle of \"total correction\" of all the cardiac defects was followed so that in addition to closure of the septal defect each patient required one or more additional operative procedures such as resection of left ventricular infarct or aneurysm, mitral valve replacement or coronary artery vein bypass grafts. All six patients survived operation but one died four weeks postoperatively from perforation of an acute peptic ulcer. In one patient the ventricular septal defect recurred and was successfully closed four months later. The surviving patients remain well at follow-up."}
{"id": "PMID:1085599", "title": "Selective proximal vagotomy. A preliminary report.", "content": "Eearly results of a prospective clinical trial of selective proximal vagotomy (SPV) with or without pyloroplasty are reported. The total number of patients was 41, of whom 39 had chronic duodenal ulcer, one gastric ulcer and one heamorrhage gastritis. The follow-up period in our preliminary series now averages 18 months. Recurrent duodenal ulcer appeared in two patients with proved incomplete vagotomy (5%), and two other patients suffered postoperatively from gastric retention. All the symptomatic patients underwent SPV without pyloroplasty. The promising early results warrant continuation of our trial in order to asses the role of SPV in the surgical treatment of duodenal ulcer.", "contents": "Selective proximal vagotomy. A preliminary report. Eearly results of a prospective clinical trial of selective proximal vagotomy (SPV) with or without pyloroplasty are reported. The total number of patients was 41, of whom 39 had chronic duodenal ulcer, one gastric ulcer and one heamorrhage gastritis. The follow-up period in our preliminary series now averages 18 months. Recurrent duodenal ulcer appeared in two patients with proved incomplete vagotomy (5%), and two other patients suffered postoperatively from gastric retention. All the symptomatic patients underwent SPV without pyloroplasty. The promising early results warrant continuation of our trial in order to asses the role of SPV in the surgical treatment of duodenal ulcer."}
{"id": "PMID:1085600", "title": "[Study by gel electrophoresis, of alpha chains and of CNBr peptides of collagen from epiphyseal cartilage in chondrodysplasia].", "content": "The alpha chains and the major CNBr - derived peptides of collagen of growth cartilage were studied in the following syndromes: thanatophoric dwarfism, pseudothanatophoric dwarfism, achondroplasia, pseudoachondroplasia, diastrophic dwarfism, metatropic dwarfism, Kniest disease, parastremmatic dwarfism, multiple exostoses, Blount disease and pycnodysostosis. After extraction of proteoglycans the collagen was solubilized by limited pepsin digestion purified, and the alpha chains were analysed by electrophoresis. The major CNBr - derived peptides were obtained by cleaving directly the cartilage after proteoglycan extraction. In some syndromes purified collagen was also cleaved. The CNBr peptides were analyzed by disc electrophoresis in SDS-polyacrylamide. Human normal growth cartilage and baboon cartilage were used as controls. The pattern of alpha chains and of major CNBr peptides was similar in all the cases studied, except one case of lethal diastrophic dwarfism in which the pattern of peptides showed the presence of type I collagen in quantities detectable by the present method. However in a milder case of diastrophic dwarfism the pattern of CNBr peptides was found normal. The present study does not exclude possible abnormalities of collagen at a higher lever of supramolecular organization in osteochondrodysplasias.", "contents": "[Study by gel electrophoresis, of alpha chains and of CNBr peptides of collagen from epiphyseal cartilage in chondrodysplasia]. The alpha chains and the major CNBr - derived peptides of collagen of growth cartilage were studied in the following syndromes: thanatophoric dwarfism, pseudothanatophoric dwarfism, achondroplasia, pseudoachondroplasia, diastrophic dwarfism, metatropic dwarfism, Kniest disease, parastremmatic dwarfism, multiple exostoses, Blount disease and pycnodysostosis. After extraction of proteoglycans the collagen was solubilized by limited pepsin digestion purified, and the alpha chains were analysed by electrophoresis. The major CNBr - derived peptides were obtained by cleaving directly the cartilage after proteoglycan extraction. In some syndromes purified collagen was also cleaved. The CNBr peptides were analyzed by disc electrophoresis in SDS-polyacrylamide. Human normal growth cartilage and baboon cartilage were used as controls. The pattern of alpha chains and of major CNBr peptides was similar in all the cases studied, except one case of lethal diastrophic dwarfism in which the pattern of peptides showed the presence of type I collagen in quantities detectable by the present method. However in a milder case of diastrophic dwarfism the pattern of CNBr peptides was found normal. The present study does not exclude possible abnormalities of collagen at a higher lever of supramolecular organization in osteochondrodysplasias."}
{"id": "PMID:1085601", "title": "[De novo trisomy 4p by 4p isochromosome].", "content": "A new example of trisomy 4p is reported, due in this case to formation of an isochromosome i(4p). The long arm of chromosome 4 was translocated onto the short arm of chromosome 1.", "contents": "[De novo trisomy 4p by 4p isochromosome]. A new example of trisomy 4p is reported, due in this case to formation of an isochromosome i(4p). The long arm of chromosome 4 was translocated onto the short arm of chromosome 1."}
{"id": "PMID:1085602", "title": "[Reciprocal translocation in the family of 2 prositas with partial trisomy of the chromosome 7q].", "content": "Two patients with partial trisomy 7q are reported. The fathers of both patients are distantly related and carry the same balanced translocation - 46,XY,rep(4;7) (q35;q32).", "contents": "[Reciprocal translocation in the family of 2 prositas with partial trisomy of the chromosome 7q]. Two patients with partial trisomy 7q are reported. The fathers of both patients are distantly related and carry the same balanced translocation - 46,XY,rep(4;7) (q35;q32)."}
{"id": "PMID:1085603", "title": "[Partial trisomy of the long arm of the chromosme 11 by malsegregation of a maternal translocation t(11;22)(q23 1;q1 11)].", "content": "A case of partial trisomy for the long arm of chromosome 11 due to malsegregation of a balanced maternal translocation is reported.", "contents": "[Partial trisomy of the long arm of the chromosme 11 by malsegregation of a maternal translocation t(11;22)(q23 1;q1 11)]. A case of partial trisomy for the long arm of chromosome 11 due to malsegregation of a balanced maternal translocation is reported."}
{"id": "PMID:1085604", "title": "[Localization of the gene of the glyceraldehyde 3 phosphate dehydrogenase on the distal segment of the short arm of the chromosome 12].", "content": "The enzymatic activity of G3PD and LDH-B in erythrocytes has been assayed in three patients carrying a rearrangement of the short arm of chromosome 12. The first patient is trisomic for the whole short arm. The second is trisomic only for the distal part and the third is monosomic for the proximal part. G3PD: Activity increased in patients 1 and 2 and normal in patient 3. LDH-B: Activity increased in patients 1 and 2 and decreased in patient 3. From these observations it is concluded that the erythrocytic G3PD locus is localized on the distal part of 12p, between 12p12.2 and 12 pter, and that LDH-B locus is on the middle third between 12p12.1 and 12p12.2", "contents": "[Localization of the gene of the glyceraldehyde 3 phosphate dehydrogenase on the distal segment of the short arm of the chromosome 12]. The enzymatic activity of G3PD and LDH-B in erythrocytes has been assayed in three patients carrying a rearrangement of the short arm of chromosome 12. The first patient is trisomic for the whole short arm. The second is trisomic only for the distal part and the third is monosomic for the proximal part. G3PD: Activity increased in patients 1 and 2 and normal in patient 3. LDH-B: Activity increased in patients 1 and 2 and decreased in patient 3. From these observations it is concluded that the erythrocytic G3PD locus is localized on the distal part of 12p, between 12p12.2 and 12 pter, and that LDH-B locus is on the middle third between 12p12.1 and 12p12.2"}
{"id": "PMID:1085605", "title": "[Partial deletion of the short arm of the chromosome 9].", "content": "Monosomy 9p is reported in a boy with trigonocephaly and advanced bone age.", "contents": "[Partial deletion of the short arm of the chromosome 9]. Monosomy 9p is reported in a boy with trigonocephaly and advanced bone age."}
{"id": "PMID:1085606", "title": "[Genealogic proximity and genetic proximity].", "content": "Data collected concerning the Kel Kummer Touareg tribe permitted the characterization of each person by (a) a \"geneological profile\", by which the genetic contributions made to him by various of the founders of the tribe can be seen; and (b) a \"hematological profile\", which shows the different alleles existing in the tribe which have come to him. The study of the correlation between these two approaches shows that, overall, a person is defined better by his membership in a population than by his geneological network.", "contents": "[Genealogic proximity and genetic proximity]. Data collected concerning the Kel Kummer Touareg tribe permitted the characterization of each person by (a) a \"geneological profile\", by which the genetic contributions made to him by various of the founders of the tribe can be seen; and (b) a \"hematological profile\", which shows the different alleles existing in the tribe which have come to him. The study of the correlation between these two approaches shows that, overall, a person is defined better by his membership in a population than by his geneological network."}
{"id": "PMID:1085607", "title": "[Compared cytologic study of (in situ and isolated) metaphasic chromosomes from KB cells, after incorporation of 5 bromodeoxyuridine (5 BrdU)].", "content": "Substitution of thymidine by 5 BrdU indduces the same morphological and cytological changes in in situ and isolated metaphase chromosomes of KB cells: a dichro\u00efc fluorescence and a difference in the affinity of each chromatid for Giemsa stain. However, the precursor does not seem to modify the appearance of G or Q bands in in situ and isolated chromosomes. The fibrilar unit which can be seen with electronic microscopy, shows no difference before and after 5-BrdU incorporation.", "contents": "[Compared cytologic study of (in situ and isolated) metaphasic chromosomes from KB cells, after incorporation of 5 bromodeoxyuridine (5 BrdU)]. Substitution of thymidine by 5 BrdU indduces the same morphological and cytological changes in in situ and isolated metaphase chromosomes of KB cells: a dichro\u00efc fluorescence and a difference in the affinity of each chromatid for Giemsa stain. However, the precursor does not seem to modify the appearance of G or Q bands in in situ and isolated chromosomes. The fibrilar unit which can be seen with electronic microscopy, shows no difference before and after 5-BrdU incorporation."}
{"id": "PMID:1085608", "title": "Digestive haemorrhages induced by traumatic cerebral lesions.", "content": "Three cases of severe craniocerebral injuries are presented wherein haemorrhagic types of digestive lesions occured at various intervals. Commonly, the cerebral lesions involve manily the rhinencephalic structures, whereas the digestive disturbances consisted mainly of vasodilatation and vasparalysis. Thus, a relationship between rhiencephalic function and digestive haemorrhages is suggested.", "contents": "Digestive haemorrhages induced by traumatic cerebral lesions. Three cases of severe craniocerebral injuries are presented wherein haemorrhagic types of digestive lesions occured at various intervals. Commonly, the cerebral lesions involve manily the rhinencephalic structures, whereas the digestive disturbances consisted mainly of vasodilatation and vasparalysis. Thus, a relationship between rhiencephalic function and digestive haemorrhages is suggested."}
{"id": "PMID:1085609", "title": "Neonatal pyridoxine responsive convulsions due to isoniazid therapy.", "content": "A 17-day-old infant on isoniazid therapy 13 mg/kg daily from birth because of maternal tuberculosis was admitted after 4 days of clonic fits. No underlying infective or biochemical cause could be found. The fits ceased within 4 hours of administering intramuscular pyridoxine, suggesting an aetiology of pyridoxine deficiency secondary to isoniazid medication.", "contents": "Neonatal pyridoxine responsive convulsions due to isoniazid therapy. A 17-day-old infant on isoniazid therapy 13 mg/kg daily from birth because of maternal tuberculosis was admitted after 4 days of clonic fits. No underlying infective or biochemical cause could be found. The fits ceased within 4 hours of administering intramuscular pyridoxine, suggesting an aetiology of pyridoxine deficiency secondary to isoniazid medication."}
{"id": "PMID:1085610", "title": "Alpha 1-antitrypsin deficiency and infantile liver disease.", "content": "Infantile liver disease with deficiency of serum alpha1-antitrypsin is illustrated by a description of the clinical, biochemical, and pathological findings in two affected families. The simplicity of the diagnostic tests is emphasized. Review of 61 biopsies of liver from children and adolescents provided a further 3 cases. It is prudent to exclude this metabolic defect in children with a history of \"neonatal hepatitis\".", "contents": "Alpha 1-antitrypsin deficiency and infantile liver disease. Infantile liver disease with deficiency of serum alpha1-antitrypsin is illustrated by a description of the clinical, biochemical, and pathological findings in two affected families. The simplicity of the diagnostic tests is emphasized. Review of 61 biopsies of liver from children and adolescents provided a further 3 cases. It is prudent to exclude this metabolic defect in children with a history of \"neonatal hepatitis\"."}
{"id": "PMID:1085611", "title": "Screening for cystic fibrosis by analysis of serum protein in faeces.", "content": "Faecal specimens from 51 infants free of cystic fibrosis (CF) and from 9 infants with the disease were analysed for albumin and alpha1-antitrypsin content. Faeces from infants with no CF had a mean albumin content of less than 0-1 mg/g dry weight and a mean albumin: alpha1-antitrypsin ratio value of less than 0-1. Faeces from infants with CF had, with one exception, an albumin content of more than 2-0 mg/g dry weight and a ratio value greater than 3-0. It was subsequently found that the duodenal aspirate from the child with CF but whose faeces had a low albumin content and ratio value, had tryptic activity though at a much reduced level compared to the activity in aspirates from healthy infants.", "contents": "Screening for cystic fibrosis by analysis of serum protein in faeces. Faecal specimens from 51 infants free of cystic fibrosis (CF) and from 9 infants with the disease were analysed for albumin and alpha1-antitrypsin content. Faeces from infants with no CF had a mean albumin content of less than 0-1 mg/g dry weight and a mean albumin: alpha1-antitrypsin ratio value of less than 0-1. Faeces from infants with CF had, with one exception, an albumin content of more than 2-0 mg/g dry weight and a ratio value greater than 3-0. It was subsequently found that the duodenal aspirate from the child with CF but whose faeces had a low albumin content and ratio value, had tryptic activity though at a much reduced level compared to the activity in aspirates from healthy infants."}
{"id": "PMID:1085612", "title": "Mode of action of veratridine on perfused frog ventricle.", "content": "Veratridine exerts a biphasic action on the perfused frog ventricle. The initial immediate response is a depression, which is maximal at 10(-10) M. The initial depression is followed by a late potentiation of contraction which is maximal at 10(-6) to 10(-7) M veratridine. Reduction of external calcium from 1.0 mM to 0.5 mM prevents the initial depression and allows only the potentiation of contraction to be observed. The force of contraction in 0.5 mM Ca2+ is increased by veratridine to 200% of the contraction observed at 1.0 mM Ca2+. The primary site for the enhancement of contraction in the frog ventricle by veratridine is the adrenergic nerve fibers whose increase in excitability leads to an increase in epinephrine release. The beta blocker sotalol at 10(-6) M prevents the increase in contractility by veratridine in 0.4 mM Ca2+. Calcium itself depressed potentiation of contractility by veratridine by stabilizing the adrenergic nerves and antagonizing the labilizing action of veratridine. The mode of action of veratridine on the frog ventricle is attributed to the enhancement of transmitter release from cholinergic and adrenergic nerve fibers.", "contents": "Mode of action of veratridine on perfused frog ventricle. Veratridine exerts a biphasic action on the perfused frog ventricle. The initial immediate response is a depression, which is maximal at 10(-10) M. The initial depression is followed by a late potentiation of contraction which is maximal at 10(-6) to 10(-7) M veratridine. Reduction of external calcium from 1.0 mM to 0.5 mM prevents the initial depression and allows only the potentiation of contraction to be observed. The force of contraction in 0.5 mM Ca2+ is increased by veratridine to 200% of the contraction observed at 1.0 mM Ca2+. The primary site for the enhancement of contraction in the frog ventricle by veratridine is the adrenergic nerve fibers whose increase in excitability leads to an increase in epinephrine release. The beta blocker sotalol at 10(-6) M prevents the increase in contractility by veratridine in 0.4 mM Ca2+. Calcium itself depressed potentiation of contractility by veratridine by stabilizing the adrenergic nerves and antagonizing the labilizing action of veratridine. The mode of action of veratridine on the frog ventricle is attributed to the enhancement of transmitter release from cholinergic and adrenergic nerve fibers."}
{"id": "PMID:1085613", "title": "The anti-inflammatory and analgesic effects of norvedan a novel, non-steroidal agent.", "content": "Norvedan (2-phenyl-4-p-chlorophenyl-thiazole-5-ylacetic acid) has been investigated for anti-inflammatory activity using an 'adjuvant arthirits' in rats and for analgesic activity using a writhing test in mice and a pressure test in rats. At oral doses of 10, 30 and 100 mg/kg Norvedan caused a dose-related inhibition of the primary swelling of the paw injected with adjuvant and the development of secondary lesions was also inhibited. Writhing induced in mice by i.p. administered acetic acid was also reduced by Norvedan at oral doses of 30 mg/kg and above and the response was related to dose. Against pain induced in rats by application of pressure to an inflamed paw Norvedan alsp showed good activity which was present for at least 6 hr after dosing.", "contents": "The anti-inflammatory and analgesic effects of norvedan a novel, non-steroidal agent. Norvedan (2-phenyl-4-p-chlorophenyl-thiazole-5-ylacetic acid) has been investigated for anti-inflammatory activity using an 'adjuvant arthirits' in rats and for analgesic activity using a writhing test in mice and a pressure test in rats. At oral doses of 10, 30 and 100 mg/kg Norvedan caused a dose-related inhibition of the primary swelling of the paw injected with adjuvant and the development of secondary lesions was also inhibited. Writhing induced in mice by i.p. administered acetic acid was also reduced by Norvedan at oral doses of 30 mg/kg and above and the response was related to dose. Against pain induced in rats by application of pressure to an inflamed paw Norvedan alsp showed good activity which was present for at least 6 hr after dosing."}
{"id": "PMID:1085614", "title": "Preliminary studies on blastic transformation of peripheral blood lymphocytes in bursectomized chicks.", "content": "Lymphocytes from six-week-old chicks which had been bursectomized when three days old showed greater tendency to blastic transformation stimulated by phytohemagglutinin (PHA) than those from control chicks. The observations suggest the possibility of B lymphocytes influencing immunological reactivity of T lymphocytes.", "contents": "Preliminary studies on blastic transformation of peripheral blood lymphocytes in bursectomized chicks. Lymphocytes from six-week-old chicks which had been bursectomized when three days old showed greater tendency to blastic transformation stimulated by phytohemagglutinin (PHA) than those from control chicks. The observations suggest the possibility of B lymphocytes influencing immunological reactivity of T lymphocytes."}
{"id": "PMID:1085615", "title": "Comparison of modifications of the rosette test for detecting T lymphocytes.", "content": "Experiments were carried out with blood lymphocytes from 30 healthy subjects and 70 patients with chronic lymphocytic leukemia (CLL). Results of the rosette test with sheep red blood cells (SRBC) were compared by the methods of: I) Wybran (incubation at room temperature, reading after 3 hr); II) Jondal (incubation at temp. 310.15K (37 degrees C) and 277.15K (4 degrees C), reading after 24 hr; III) Weiner et al. (neuraminidase-treated SRBC, incubation at 273.15K (0 degrees C), reading after 15 min; IV) as in method III, but reading after 24 hr. The method of Wybran gave lowest results, statistically significantly differing from the results obtained by the other methods. The other modifications gave results that did not differ significantly. Neuraminidase treatment of SRBC accelerates formation of stable rosettes and this method is recommended in cases where a brief period of incubation is required. In other cases, the method of Jondal may be used.", "contents": "Comparison of modifications of the rosette test for detecting T lymphocytes. Experiments were carried out with blood lymphocytes from 30 healthy subjects and 70 patients with chronic lymphocytic leukemia (CLL). Results of the rosette test with sheep red blood cells (SRBC) were compared by the methods of: I) Wybran (incubation at room temperature, reading after 3 hr); II) Jondal (incubation at temp. 310.15K (37 degrees C) and 277.15K (4 degrees C), reading after 24 hr; III) Weiner et al. (neuraminidase-treated SRBC, incubation at 273.15K (0 degrees C), reading after 15 min; IV) as in method III, but reading after 24 hr. The method of Wybran gave lowest results, statistically significantly differing from the results obtained by the other methods. The other modifications gave results that did not differ significantly. Neuraminidase treatment of SRBC accelerates formation of stable rosettes and this method is recommended in cases where a brief period of incubation is required. In other cases, the method of Jondal may be used."}
{"id": "PMID:1085616", "title": "Influence of D-penicillamine and beta-aminopropionitril on the metamorphosis of Rana temporaria.", "content": "Tadpoles of Rana temporaria (size 5-7 mm) were kept in solutions of D-penicillamine (DPA), beta-aminopropionitril (betaAPN), and L-thyroxine at different concentrations for 18 days. The influence of the substances on metamorphosis was investigated. In low concentrations (1 or 10 mg/100 ml) D-penicillamine accelerates the process of metamorphosis. At these concentrations investigated skeletal malformations could not be observed. A content of 100 mg of DPA/100 ml is toxic. After 8 days of exposure only 4 of the 15 test animals had survived. In all concentrations investigated, beta-aminopropionitril has a toxic action. In low concentration (1 mg/100 ml) deformities are found which could be described as bending of the femur and twisting of the whole hind limb. The animals are non-viable, they die shortly after termination of metamorphosis. Higher concentrations (10 or 100 mg/100 ml) are lethal within the first 10 days. L-thyroxine (0.01 mg/100 ml) shows the wellknown metamorphosis-accelerating effect.", "contents": "Influence of D-penicillamine and beta-aminopropionitril on the metamorphosis of Rana temporaria. Tadpoles of Rana temporaria (size 5-7 mm) were kept in solutions of D-penicillamine (DPA), beta-aminopropionitril (betaAPN), and L-thyroxine at different concentrations for 18 days. The influence of the substances on metamorphosis was investigated. In low concentrations (1 or 10 mg/100 ml) D-penicillamine accelerates the process of metamorphosis. At these concentrations investigated skeletal malformations could not be observed. A content of 100 mg of DPA/100 ml is toxic. After 8 days of exposure only 4 of the 15 test animals had survived. In all concentrations investigated, beta-aminopropionitril has a toxic action. In low concentration (1 mg/100 ml) deformities are found which could be described as bending of the femur and twisting of the whole hind limb. The animals are non-viable, they die shortly after termination of metamorphosis. Higher concentrations (10 or 100 mg/100 ml) are lethal within the first 10 days. L-thyroxine (0.01 mg/100 ml) shows the wellknown metamorphosis-accelerating effect."}
{"id": "PMID:1085617", "title": "Haematemesis and Melaena: surgical management.", "content": "A prospective study of the surgical management of 100 consecutive patients with benign, non-variceal upper gastrointestinal bleeding is presented. The manner of presentation, precipitating factors, investigations and associated medical problems are discussed. Chronic duodenal ulceration was the most common cause of haemorrhage. Vagotomy and drainage with oversewing or excision of the bleeding ulcer was the surgical procedure performed in 71 of the patients in the series. The incidence of recurrent bleeding was 7%; no patient in this category required further operation. The mortality rate was 6%, and there were no deaths recorded in the patients who underwent vagotomy and drainage. The reasons for the relatively low surgical mortality are discussed.", "contents": "Haematemesis and Melaena: surgical management. A prospective study of the surgical management of 100 consecutive patients with benign, non-variceal upper gastrointestinal bleeding is presented. The manner of presentation, precipitating factors, investigations and associated medical problems are discussed. Chronic duodenal ulceration was the most common cause of haemorrhage. Vagotomy and drainage with oversewing or excision of the bleeding ulcer was the surgical procedure performed in 71 of the patients in the series. The incidence of recurrent bleeding was 7%; no patient in this category required further operation. The mortality rate was 6%, and there were no deaths recorded in the patients who underwent vagotomy and drainage. The reasons for the relatively low surgical mortality are discussed."}
{"id": "PMID:1085625", "title": "Lymphocytotoxic antibody activity in cryoprecipitates from serum of patients with SLE.", "content": "Complement-dependent cytotoxic activity against normal human peripheral blood lymphocytes was detected in 11 of 17 cryoprecipitates from the serum of patients with systemic lupus erythematosus (SLE). The lymphocytotoxicity was eliminated by treatment with 2-mercaptoethanol and iodoacetamide, and it was inhibited by antibody to human IgM but not anti-IgG. The titers of lymphocytotoxic activity in the cryoprecipitates were roughly proportional to the corresponding serum titers, but when they were normalized for IgM concentration it was apparent that selective concentration of lymphocytotoxic antibody occurred in the cryoglobulins. The relationship between cryoprecipitable lymphocytotoxicity and a number of laboratory and clinical parameters of SLE was studied. The amount of protein in the cryoprecipitates,  which was greatest in patients with significant renal disease, correlated with a reduction of serum complement and the amount of antibody to DNA. However the lymphocytotoxic activity of the cryoglobulins did not correlate with the severity of SLE. The titer of lymphocytotoxic antibody was independent of a) the presence or absence of active lupus nephritis, b) the total protein or immunoglobulin content of the cryoprecipitates, c) serum complement levels, and d) the amount of circulating antibody to DNA. These findings cast doubt upon the pathogenetic significance of cryoprecipitable lymphocytotoxic antibody.", "contents": "Lymphocytotoxic antibody activity in cryoprecipitates from serum of patients with SLE. Complement-dependent cytotoxic activity against normal human peripheral blood lymphocytes was detected in 11 of 17 cryoprecipitates from the serum of patients with systemic lupus erythematosus (SLE). The lymphocytotoxicity was eliminated by treatment with 2-mercaptoethanol and iodoacetamide, and it was inhibited by antibody to human IgM but not anti-IgG. The titers of lymphocytotoxic activity in the cryoprecipitates were roughly proportional to the corresponding serum titers, but when they were normalized for IgM concentration it was apparent that selective concentration of lymphocytotoxic antibody occurred in the cryoglobulins. The relationship between cryoprecipitable lymphocytotoxicity and a number of laboratory and clinical parameters of SLE was studied. The amount of protein in the cryoprecipitates,  which was greatest in patients with significant renal disease, correlated with a reduction of serum complement and the amount of antibody to DNA. However the lymphocytotoxic activity of the cryoglobulins did not correlate with the severity of SLE. The titer of lymphocytotoxic antibody was independent of a) the presence or absence of active lupus nephritis, b) the total protein or immunoglobulin content of the cryoprecipitates, c) serum complement levels, and d) the amount of circulating antibody to DNA. These findings cast doubt upon the pathogenetic significance of cryoprecipitable lymphocytotoxic antibody."}
{"id": "PMID:1085626", "title": "Study of thymic factors. II. Failure of thymosin to alter the natural history of NZB and NZB/NZW mice.", "content": "The effects of daily injections of thymosin, bovine fraction V, on the natural history of NZB and NZB/NZW F1 mice were investigated. With the use of several dose schedules, no significant differences were discovered in treated versus control groups when survival, autoantibodies, and mitogen responsiveness were compared. These results provide further evidence that thymosin may have little or no role in the treatment of the autoimmune disease of New Zealand mice. More encouraging research in thymic extracts and their measurement is necessary before clinical trials in SLE are considered.", "contents": "Study of thymic factors. II. Failure of thymosin to alter the natural history of NZB and NZB/NZW mice. The effects of daily injections of thymosin, bovine fraction V, on the natural history of NZB and NZB/NZW F1 mice were investigated. With the use of several dose schedules, no significant differences were discovered in treated versus control groups when survival, autoantibodies, and mitogen responsiveness were compared. These results provide further evidence that thymosin may have little or no role in the treatment of the autoimmune disease of New Zealand mice. More encouraging research in thymic extracts and their measurement is necessary before clinical trials in SLE are considered."}
{"id": "PMID:1085627", "title": "Antibodies to dAT detected by membrane filtration.", "content": "Sera from patients with active systemic lupus erythematosus caused the retention of the radioactively labeled synthetic polynucleotide 14C-dAT on cellulose nitrate filters. The amount of 14C-dAT bound correlated with the presence of hypocomplementemia and active renal disease. Sera containing only anti-single-stranded DNA antibodies did not bind 14C-dAT but did bind 125I DNA from E coli that had been prefiltered to remove ssDNA contamination. Inhibition experiments also indicated the presence of antigenic differences between 14C-dAT and prefiltered 125I-E coli DNA. The data suggest that dAT carries one of the major antigenic specificities for antinative DNA antibodies in SLE, and that cellulose nitrate filtration fails to remove a significant proportion of contaminating single-stranded DNA determinants in non-synthetic DNA.", "contents": "Antibodies to dAT detected by membrane filtration. Sera from patients with active systemic lupus erythematosus caused the retention of the radioactively labeled synthetic polynucleotide 14C-dAT on cellulose nitrate filters. The amount of 14C-dAT bound correlated with the presence of hypocomplementemia and active renal disease. Sera containing only anti-single-stranded DNA antibodies did not bind 14C-dAT but did bind 125I DNA from E coli that had been prefiltered to remove ssDNA contamination. Inhibition experiments also indicated the presence of antigenic differences between 14C-dAT and prefiltered 125I-E coli DNA. The data suggest that dAT carries one of the major antigenic specificities for antinative DNA antibodies in SLE, and that cellulose nitrate filtration fails to remove a significant proportion of contaminating single-stranded DNA determinants in non-synthetic DNA."}
{"id": "PMID:1085628", "title": "Lymphocyte response to mitogens in progressive systemic sclerosis.", "content": "Lymphocyte responses to the mitogens phytohemagglutinin P(PHA-P), concanavallin A (Con A), and pokeweed (PWM) were studied in 18 patients with progressive systemic sclerosis (PSS). A subgroup of these patients with multisystem involvement showed a significantly decreased lymphocyte response to both Con A and PWM when compared to normal controls. However those PSS patients with myositis, although having multisystem involvement, had normal lymphocyte response to all three mitogens. PHA-P stumulation was normal in all PSS patients. Antinuclear antibodies, elevated sedimentation rates, and positive latex fixation were noted only in the multisystem disease group with abnormal lymphocyte function.", "contents": "Lymphocyte response to mitogens in progressive systemic sclerosis. Lymphocyte responses to the mitogens phytohemagglutinin P(PHA-P), concanavallin A (Con A), and pokeweed (PWM) were studied in 18 patients with progressive systemic sclerosis (PSS). A subgroup of these patients with multisystem involvement showed a significantly decreased lymphocyte response to both Con A and PWM when compared to normal controls. However those PSS patients with myositis, although having multisystem involvement, had normal lymphocyte response to all three mitogens. PHA-P stumulation was normal in all PSS patients. Antinuclear antibodies, elevated sedimentation rates, and positive latex fixation were noted only in the multisystem disease group with abnormal lymphocyte function."}
{"id": "PMID:1085631", "title": "New data on the mechanism and speed of action of nitroimidazoles on prokaryota and eukaryota with and without mitochondria.", "content": "The spectrum of action of nitroimidazoles ranges from anaerobic bacteria to aerobic bacteria which are also capable of growth, although sometimes with difficulty, in anaerobic atmosphere and to protozoa without and with mitochondria; in the latter case the anti-protozoal activity depends on the importance of the extramitochondrial enzymes which respond to nitroimidazoles. The more anaerobic the organisms, the greater their sensitivity. In the most sensitive protozoa, the trichomonads, most of the enzymatic reactions inhibited by these drugs take place in the hydrogenosomes; these are cytoplasmatic organelles surrounded by a membrane whose disintegration, in fact, is the first visible effect of the action of the nitroimidazoles. Various nitroimidazoles act with different speed on these and other protozoa, whose membranes can difinitely possess different degrees of permeability towards substances with different chemico-physical characteristics. In the treatment of trichomoniasis, the specific speed of action of a drug is perhaps as important as its half-life in man.", "contents": "New data on the mechanism and speed of action of nitroimidazoles on prokaryota and eukaryota with and without mitochondria. The spectrum of action of nitroimidazoles ranges from anaerobic bacteria to aerobic bacteria which are also capable of growth, although sometimes with difficulty, in anaerobic atmosphere and to protozoa without and with mitochondria; in the latter case the anti-protozoal activity depends on the importance of the extramitochondrial enzymes which respond to nitroimidazoles. The more anaerobic the organisms, the greater their sensitivity. In the most sensitive protozoa, the trichomonads, most of the enzymatic reactions inhibited by these drugs take place in the hydrogenosomes; these are cytoplasmatic organelles surrounded by a membrane whose disintegration, in fact, is the first visible effect of the action of the nitroimidazoles. Various nitroimidazoles act with different speed on these and other protozoa, whose membranes can difinitely possess different degrees of permeability towards substances with different chemico-physical characteristics. In the treatment of trichomoniasis, the specific speed of action of a drug is perhaps as important as its half-life in man."}
{"id": "PMID:1085629", "title": "Carcinomatous polyarthritis. The presenting symptom of an ovarian tumor and association with a platelet activating factor.", "content": "A 59-year-old white female presented with an acute polyarthritis, fever, and mental confusion. Accompanying blood changes included positive tests for rheumatoid factor and antinuclear factor, anemia, leukocytosis, thrombocytosis, and a circulating factor that stimulated serotonin release from normal platelets. Exploratory laporatomy for a mobile pelvic mass revealed an adenocarcinoma of the left ovary which was completely resected. Shortly thereafter the patient's acute symptoms subsided. After 2 years of follow-up the patient has remained free of symptoms and the serologic changes have reverted to normal.", "contents": "Carcinomatous polyarthritis. The presenting symptom of an ovarian tumor and association with a platelet activating factor. A 59-year-old white female presented with an acute polyarthritis, fever, and mental confusion. Accompanying blood changes included positive tests for rheumatoid factor and antinuclear factor, anemia, leukocytosis, thrombocytosis, and a circulating factor that stimulated serotonin release from normal platelets. Exploratory laporatomy for a mobile pelvic mass revealed an adenocarcinoma of the left ovary which was completely resected. Shortly thereafter the patient's acute symptoms subsided. After 2 years of follow-up the patient has remained free of symptoms and the serologic changes have reverted to normal."}
{"id": "PMID:1085632", "title": "[On the pharmacodynamics of alpha- and beta-escin after oral application (author's transl)].", "content": "Contrary to the almost water insoluble crystalline beta-escinic acid, the water soluble forms of escin -- such as alpha-escinic acid or its salts, Na-b-escinate and the amorphous beta-escinic acid -- are so well absorbed by the gastro-intestinal tract that the effects after oral application could be compared with those of the reference substances furosemide, hydrocortisone, acetylsalicylic acid, azapropazone and phenylbutazone. Escin was tested in the stasis edema, cotton-pellet-granuloma, and UV-erythema, i.e., in test models which seem specially suited to characterize the properties of this substance. In these tests which reflect both the prophylactic and the therapeutic treatment escin has proven antiexudative-antiphlogistic effects. They are based on a favorable influence of permeability and diuresis.", "contents": "[On the pharmacodynamics of alpha- and beta-escin after oral application (author's transl)]. Contrary to the almost water insoluble crystalline beta-escinic acid, the water soluble forms of escin -- such as alpha-escinic acid or its salts, Na-b-escinate and the amorphous beta-escinic acid -- are so well absorbed by the gastro-intestinal tract that the effects after oral application could be compared with those of the reference substances furosemide, hydrocortisone, acetylsalicylic acid, azapropazone and phenylbutazone. Escin was tested in the stasis edema, cotton-pellet-granuloma, and UV-erythema, i.e., in test models which seem specially suited to characterize the properties of this substance. In these tests which reflect both the prophylactic and the therapeutic treatment escin has proven antiexudative-antiphlogistic effects. They are based on a favorable influence of permeability and diuresis."}
{"id": "PMID:1085639", "title": "Peripheral blood T- and B-lymphocytes in operating theatre personnel.", "content": "The effect of operating theatre work on peripheral blood T- and B-lymphocytes was studied by the rosette method. There was no statistically significant difference between operating theatre personnel and the controls in the number of T- and B-lymphocytes. This study supports the view that work in operating theatres does not cause changes in the immune response.", "contents": "Peripheral blood T- and B-lymphocytes in operating theatre personnel. The effect of operating theatre work on peripheral blood T- and B-lymphocytes was studied by the rosette method. There was no statistically significant difference between operating theatre personnel and the controls in the number of T- and B-lymphocytes. This study supports the view that work in operating theatres does not cause changes in the immune response."}
{"id": "PMID:1085640", "title": "An anti-A-like lectin of Rana catesbiana eggs showing unusual reactivity.", "content": "A lectin was isolated from Rana catesbiana eggs that agglutinated blood group A-erythrocytes but did not agglutinate blood group B- or 0-erythrocytes. The lectin was purified by Sephadex G-75 gel filtration and by acrylamide gel electrophoresis at pH 4.3 and was proved to be homogeneous on electrophoresis, and the molecular weight was determined as 210 000. The specificity of A-like activity seems to direct towards three monosaccharide units: GalNAcalpha1 leads to 3(or 4)-Galbeta1 leads to 4(or 3)GlcNAcbeta1 leads to R based on inhibition of A-like hemagglutination by various monosaccharides, oligosaccharides and glycolipids, and based on precipitin reaction with various glycolipids and glycoproteins with known structures. Uniquely, A-like agglutination was inhibited not only by alpha-N-acetylgalactosamine analogs but also by N-acetyllactosamine analogs. The lectin showed therefore, two correlated specificities: one directed towards alpha-N-acetylgalactosamine residue at the terminal, and the other towards the subterminal Galbeta1 leads to 4betaGlcNAc (N-acetyllactosaminyl) residue. The reactivity due to the N-acetyllactosamine structure which is also found in erythrocyte ganglioside and in H-active chain might be blocked by sialyl or alpha-L-fucosyl substitution at the terminal, as the reactivity appeared after elimination of these sugar residues. In the A structure the reactivity due to N-acetyllactosaminyl residue seems not to be blocked by the presence of alpha-N-acetylgalactosamine at the terminal as A-agglutination was strongly inhibited by N-acetyllactosamine and its analogs. Although the lectin showed a single band on electrophoresis under different conditions, there is a possibility that the lectin may be a mixture of two proteins with different specificities as mentioned above.", "contents": "An anti-A-like lectin of Rana catesbiana eggs showing unusual reactivity. A lectin was isolated from Rana catesbiana eggs that agglutinated blood group A-erythrocytes but did not agglutinate blood group B- or 0-erythrocytes. The lectin was purified by Sephadex G-75 gel filtration and by acrylamide gel electrophoresis at pH 4.3 and was proved to be homogeneous on electrophoresis, and the molecular weight was determined as 210 000. The specificity of A-like activity seems to direct towards three monosaccharide units: GalNAcalpha1 leads to 3(or 4)-Galbeta1 leads to 4(or 3)GlcNAcbeta1 leads to R based on inhibition of A-like hemagglutination by various monosaccharides, oligosaccharides and glycolipids, and based on precipitin reaction with various glycolipids and glycoproteins with known structures. Uniquely, A-like agglutination was inhibited not only by alpha-N-acetylgalactosamine analogs but also by N-acetyllactosamine analogs. The lectin showed therefore, two correlated specificities: one directed towards alpha-N-acetylgalactosamine residue at the terminal, and the other towards the subterminal Galbeta1 leads to 4betaGlcNAc (N-acetyllactosaminyl) residue. The reactivity due to the N-acetyllactosamine structure which is also found in erythrocyte ganglioside and in H-active chain might be blocked by sialyl or alpha-L-fucosyl substitution at the terminal, as the reactivity appeared after elimination of these sugar residues. In the A structure the reactivity due to N-acetyllactosaminyl residue seems not to be blocked by the presence of alpha-N-acetylgalactosamine at the terminal as A-agglutination was strongly inhibited by N-acetyllactosamine and its analogs. Although the lectin showed a single band on electrophoresis under different conditions, there is a possibility that the lectin may be a mixture of two proteins with different specificities as mentioned above."}
{"id": "PMID:1085641", "title": "Phospholipid metabolism of stimulated lymphocytes. Comparison of the activation of acyl-CoA:lysolecithin acyltransferase with the binding of concanavalin A to thymocytes.", "content": "Calf thymocytes were isolated and incubated with concanavalin A. The effect of the mitogen on the enzyme activity of membrane-bound lysolecithin acyltransferase (acyl-CoA:1-acylglycero-3-phosphorylcholine-O-acyltransferase, EC 2.3.1.23) was determined as also the binding of 125I-labelled concanavalin A to intact cells and isolated membranes. The lysolecithin acyltransferase was found to be activated three times in microsomal membranes. The activation occurred directly after binding of concanavalin A and was temperature independent, since similar activities were found in cells treated with concanavalin A at 0 and 37 degrees C. The acyltransferase activation using increasing concentrations of concanavalin A revealed a different behaviour, as compared to the binding of concanavalin A. While the binding of concanavalin A to intact cells expressed a normal hyperbolic saturation function the activation process of the acyltransferase described a sigmoidal relationship. Correspondingly, the interaction coefficients for both functions were different (Sips coefficient for binding = 1.0 and Hill coefficient of the enzyme activation = 1.8). These results indicate that the acyltransferase activation is due to a cooperative interaction between the ligand-receptor complex and the enzyme.", "contents": "Phospholipid metabolism of stimulated lymphocytes. Comparison of the activation of acyl-CoA:lysolecithin acyltransferase with the binding of concanavalin A to thymocytes. Calf thymocytes were isolated and incubated with concanavalin A. The effect of the mitogen on the enzyme activity of membrane-bound lysolecithin acyltransferase (acyl-CoA:1-acylglycero-3-phosphorylcholine-O-acyltransferase, EC 2.3.1.23) was determined as also the binding of 125I-labelled concanavalin A to intact cells and isolated membranes. The lysolecithin acyltransferase was found to be activated three times in microsomal membranes. The activation occurred directly after binding of concanavalin A and was temperature independent, since similar activities were found in cells treated with concanavalin A at 0 and 37 degrees C. The acyltransferase activation using increasing concentrations of concanavalin A revealed a different behaviour, as compared to the binding of concanavalin A. While the binding of concanavalin A to intact cells expressed a normal hyperbolic saturation function the activation process of the acyltransferase described a sigmoidal relationship. Correspondingly, the interaction coefficients for both functions were different (Sips coefficient for binding = 1.0 and Hill coefficient of the enzyme activation = 1.8). These results indicate that the acyltransferase activation is due to a cooperative interaction between the ligand-receptor complex and the enzyme."}
{"id": "PMID:1085643", "title": "Electrophoretic mobility of lymphocytes in new born.", "content": "T lymphocytes in normal new born at the 4th day after birth have an electrophoretic mobility of 1.04, quite slower than adult normal T lymphocytes (1.13), but the same of the EM of lymphoblasts of ALL (1.04).", "contents": "Electrophoretic mobility of lymphocytes in new born. T lymphocytes in normal new born at the 4th day after birth have an electrophoretic mobility of 1.04, quite slower than adult normal T lymphocytes (1.13), but the same of the EM of lymphoblasts of ALL (1.04)."}
{"id": "PMID:1085644", "title": "Dynamics of the aromatic amino acid residues in the globular conformation of the basic pancreatic trypsin inhibitor (BPTI). II. Semi-empirical energy calculations.", "content": "The molecular conformation of the basic pancreatic trypsin inhibitor (BPTI) is known in considerable detail from both X-ray studies in single crystals and NMR studies in solution. The NMR experiments showed that the aromatic rings of the phenylalanyl and tyrosyl residues can undergo rapid rotational motions about the C beta--Cv bond. The present paper describes a model investigation of the mechanistic aspects of these intramolecular rotational motions. From calculations of the conformational energies for molecular species derived from the X-ray structure by rotations of individual aromatic rings, it was apparent that the rotational motions of the aromatics could only be understood in a flexible structure. Flexibility was simulated by allowing the protein to relax to an energetically favorable conformation for each of the different rotation states of the aromatic rings. It was then of particular interest to investigate how the perturbations caused by different rotation states of the aromatic rings were propagated in the protein structure. It was found that the rotation axes C beta--Cv were only slightly affected (delta X1 approximately less than 20 degrees. The most sizeable perturbations are caused by through space interactions with nearby atoms, which move away from the ring center and thus release the steric hindrance opposing the rotational motions. The values for the energy barriers obtained from the energy minimization are of the same order of magnitude as those measured by NMR.", "contents": "Dynamics of the aromatic amino acid residues in the globular conformation of the basic pancreatic trypsin inhibitor (BPTI). II. Semi-empirical energy calculations. The molecular conformation of the basic pancreatic trypsin inhibitor (BPTI) is known in considerable detail from both X-ray studies in single crystals and NMR studies in solution. The NMR experiments showed that the aromatic rings of the phenylalanyl and tyrosyl residues can undergo rapid rotational motions about the C beta--Cv bond. The present paper describes a model investigation of the mechanistic aspects of these intramolecular rotational motions. From calculations of the conformational energies for molecular species derived from the X-ray structure by rotations of individual aromatic rings, it was apparent that the rotational motions of the aromatics could only be understood in a flexible structure. Flexibility was simulated by allowing the protein to relax to an energetically favorable conformation for each of the different rotation states of the aromatic rings. It was then of particular interest to investigate how the perturbations caused by different rotation states of the aromatic rings were propagated in the protein structure. It was found that the rotation axes C beta--Cv were only slightly affected (delta X1 approximately less than 20 degrees. The most sizeable perturbations are caused by through space interactions with nearby atoms, which move away from the ring center and thus release the steric hindrance opposing the rotational motions. The values for the energy barriers obtained from the energy minimization are of the same order of magnitude as those measured by NMR."}
{"id": "PMID:1085645", "title": "Acquired angioedema with lymphoproliferative disorder: association of C1 inhibitor deficiency with cellular abnormality.", "content": "A patient with a lymphoproliferative disorder, angioedema, and an acquired deficiency of the inhibitor of the activated first component of complement was studied. The patient's complement profile revealed depletion of the first component of complement, which has not been seen in angioedema of the hereditary type. There was no evidence for C1-depleting activity in the patient's plasma. The majority of the patient's peripheral blood mononuclear cells resembled B cells in their memebrane receptor properties and in that they carried easily detectable immunoglobulin, predominantly IgM. However, these cells were unusual in that they phagocytosed both latex particles and C3-coated erythrocytes. Morphological study of the cells infiltrating the patient's lung revealed immature, atypical, and plasmacytoid lymphocytes and immunoblasts. Both the patient's peripheral blood mononuclear cells and a suspension of cells from the pulmonary infiltrate were capable of depleting the first component of complement and its inhibitor from homologous plasma. Normal ABO-compatible cells did not possess this property. The data suggested that the patient's abnormal lymphoid cells may have interacted with the complement system to produce a biochemical defect and a clinical syndrome closely resembling angioedema of the hereditary type.", "contents": "Acquired angioedema with lymphoproliferative disorder: association of C1 inhibitor deficiency with cellular abnormality. A patient with a lymphoproliferative disorder, angioedema, and an acquired deficiency of the inhibitor of the activated first component of complement was studied. The patient's complement profile revealed depletion of the first component of complement, which has not been seen in angioedema of the hereditary type. There was no evidence for C1-depleting activity in the patient's plasma. The majority of the patient's peripheral blood mononuclear cells resembled B cells in their memebrane receptor properties and in that they carried easily detectable immunoglobulin, predominantly IgM. However, these cells were unusual in that they phagocytosed both latex particles and C3-coated erythrocytes. Morphological study of the cells infiltrating the patient's lung revealed immature, atypical, and plasmacytoid lymphocytes and immunoblasts. Both the patient's peripheral blood mononuclear cells and a suspension of cells from the pulmonary infiltrate were capable of depleting the first component of complement and its inhibitor from homologous plasma. Normal ABO-compatible cells did not possess this property. The data suggested that the patient's abnormal lymphoid cells may have interacted with the complement system to produce a biochemical defect and a clinical syndrome closely resembling angioedema of the hereditary type."}
{"id": "PMID:1085646", "title": "Effect of ionophore X-537A on desensitization rate and tension development in potassium-depolarized muscle fibres.", "content": "The effects of the ionophore X-537A were studied on carbamylcholine (carbachol)-induced densitization and on tension development in relaxed potassium-depolarized frog sartorius muscles. 2 X-537A accelerated carbachol-induced desensitization in Ca2+-deficient solutions without having any effect on the conductance of the membrane in the absence of carbachol or on the extent of the carbachol-induced increase in conductance. 3 In Ca2+-deficient solution, the acceleration of desensitization by the ionophore was concentration-dependent. No effect was observed with concentrations less than 5 muM and maximal acceleration was evident with 10 muM. 4 The influence of X-537A on desensitization was time-dependent. At 20 muM X-537A, there was a marked acceleration of desensitization by the end of 5 min exposure. An additional gradual acceleration occurred during a 5 to 30 min treatment. No acceleration of desensitization was evident when X-537A was simultaneously applied with carbachol to the end-plate region without prior exposure to the ionophore. 5 Desensitization also was accelerated by 30 min exposure to 20 muM X-537A in solutions containing Ca2+ or deficient in both Mg2+ and Ca2+; the rate being increased 2.8-fold in Ca2+-containing solutions, 2.9-fold in Ca2+-deficient solutions containing Mg2+, and 2.5-fold in divalent cation-deficient solutions. 6 Tension development gradually occurred in relaxed potassium-depolarized muscle preparations exposed to 20 muM X-537A. The onset of tension development occurred only after approximately 25 min of exposure both in preparations kept in Ca2+-deficient or Ca2+-containing solutions. By the end of 90 min in the ionophore, the tension developed was approximately 12% and 23% of the initial potassium contracture in those preparations maintained in the Ca2+-deficient or Ca2+-containing solutions, respectively. 7 We assume that the increase in desensitization rate following exposure to X-537A results from an elevation of the intracellular Ca2+ concentration. That muscle tension gradually increased during exposure to the ionophore supports this conclusion. The acceleration of densitization by X-537A in the absence of external Ca2+ supports the view that the site of calcium acceleration is not on the external surface of the end-plate membrane either at or near the agonist-recognition site but rather on the inner surface.", "contents": "Effect of ionophore X-537A on desensitization rate and tension development in potassium-depolarized muscle fibres. The effects of the ionophore X-537A were studied on carbamylcholine (carbachol)-induced densitization and on tension development in relaxed potassium-depolarized frog sartorius muscles. 2 X-537A accelerated carbachol-induced desensitization in Ca2+-deficient solutions without having any effect on the conductance of the membrane in the absence of carbachol or on the extent of the carbachol-induced increase in conductance. 3 In Ca2+-deficient solution, the acceleration of desensitization by the ionophore was concentration-dependent. No effect was observed with concentrations less than 5 muM and maximal acceleration was evident with 10 muM. 4 The influence of X-537A on desensitization was time-dependent. At 20 muM X-537A, there was a marked acceleration of desensitization by the end of 5 min exposure. An additional gradual acceleration occurred during a 5 to 30 min treatment. No acceleration of desensitization was evident when X-537A was simultaneously applied with carbachol to the end-plate region without prior exposure to the ionophore. 5 Desensitization also was accelerated by 30 min exposure to 20 muM X-537A in solutions containing Ca2+ or deficient in both Mg2+ and Ca2+; the rate being increased 2.8-fold in Ca2+-containing solutions, 2.9-fold in Ca2+-deficient solutions containing Mg2+, and 2.5-fold in divalent cation-deficient solutions. 6 Tension development gradually occurred in relaxed potassium-depolarized muscle preparations exposed to 20 muM X-537A. The onset of tension development occurred only after approximately 25 min of exposure both in preparations kept in Ca2+-deficient or Ca2+-containing solutions. By the end of 90 min in the ionophore, the tension developed was approximately 12% and 23% of the initial potassium contracture in those preparations maintained in the Ca2+-deficient or Ca2+-containing solutions, respectively. 7 We assume that the increase in desensitization rate following exposure to X-537A results from an elevation of the intracellular Ca2+ concentration. That muscle tension gradually increased during exposure to the ionophore supports this conclusion. The acceleration of densitization by X-537A in the absence of external Ca2+ supports the view that the site of calcium acceleration is not on the external surface of the end-plate membrane either at or near the agonist-recognition site but rather on the inner surface."}
{"id": "PMID:1085650", "title": "Periodic alternating nystagmus.", "content": "Three patients with periodic alternating nystagmus (PAN) are described in detail. Digital computer methods were used to quantify their disordered eye movement in an attempt to understand the pathophysiology. One of the patients was unusual in showing rebound nystagmus with fixation and PAN without fixation. Each patient had hyperactive vestibular responses and the phase and gain of the PAN cycles were altered in a predictable fashion by vestibular stimuli. It is postulated that PAN represents cyclical firing between reciprocally connected groups of inhibitory neurons within the vestibular and oculomotor nuclei. The cyclical firing is initiated by a critical imbalance of tonic input to either group of normally functioning neurons.", "contents": "Periodic alternating nystagmus. Three patients with periodic alternating nystagmus (PAN) are described in detail. Digital computer methods were used to quantify their disordered eye movement in an attempt to understand the pathophysiology. One of the patients was unusual in showing rebound nystagmus with fixation and PAN without fixation. Each patient had hyperactive vestibular responses and the phase and gain of the PAN cycles were altered in a predictable fashion by vestibular stimuli. It is postulated that PAN represents cyclical firing between reciprocally connected groups of inhibitory neurons within the vestibular and oculomotor nuclei. The cyclical firing is initiated by a critical imbalance of tonic input to either group of normally functioning neurons."}
{"id": "PMID:1085659", "title": "The New A /New Jersey/ 76 influenza strain.", "content": "This Memorandum reviews the information available on the new strain of influenza virus, A/New Jersey/76 (Hsw1N1), that first appeared at Fort Dix, NJ, USA, in February 1976. Recommendations are given concerning measures to be taken to detect spread of this strain and to meet the challenge if an epidemic should occur.", "contents": "The New A /New Jersey/ 76 influenza strain. This Memorandum reviews the information available on the new strain of influenza virus, A/New Jersey/76 (Hsw1N1), that first appeared at Fort Dix, NJ, USA, in February 1976. Recommendations are given concerning measures to be taken to detect spread of this strain and to meet the challenge if an epidemic should occur."}
{"id": "PMID:1085660", "title": "Population replacement in Culex fatigans by means of cytoplasmic incompatibility. 2. Field cage experiments with overlapping generations.", "content": "Three experiments were carried out in field cages to test the principle of \"transport\" of a desirable gene or chromosome into a wild Culex fatigans population as a result of the sterility in cross-matings associated with cytoplasmic incompatibility. Cycling populations of Delhi origin were established in the cages and daily releases were made of the IS31B strain, which has Paris cytoplasm and carries a male-linked translocation. It was shown that, if sufficient releases were made to establish a majority of the Paris cytoplasmic type, complete replacement by this cytoplasmic type subsequently occurred. However, as a result of partial compatibility of males of the Delhi population with Paris females, \"recombinant\" males with Paris cytoplasm and no translocation were produced. In an experiment in which a continuous low rate of \"immigration\" of a strain of Delhi origin was simulated, a gradual increase of the Paris cytoplasm nontranslocated type occurred, and renewed IS31B releases were necessary after 5 months to restore the predominance of this type. The results are compared with computer predictions and discussed in relation to the transport of genes for filaria refractoriness or chromosome translocations into wild populations.", "contents": "Population replacement in Culex fatigans by means of cytoplasmic incompatibility. 2. Field cage experiments with overlapping generations. Three experiments were carried out in field cages to test the principle of \"transport\" of a desirable gene or chromosome into a wild Culex fatigans population as a result of the sterility in cross-matings associated with cytoplasmic incompatibility. Cycling populations of Delhi origin were established in the cages and daily releases were made of the IS31B strain, which has Paris cytoplasm and carries a male-linked translocation. It was shown that, if sufficient releases were made to establish a majority of the Paris cytoplasmic type, complete replacement by this cytoplasmic type subsequently occurred. However, as a result of partial compatibility of males of the Delhi population with Paris females, \"recombinant\" males with Paris cytoplasm and no translocation were produced. In an experiment in which a continuous low rate of \"immigration\" of a strain of Delhi origin was simulated, a gradual increase of the Paris cytoplasm nontranslocated type occurred, and renewed IS31B releases were necessary after 5 months to restore the predominance of this type. The results are compared with computer predictions and discussed in relation to the transport of genes for filaria refractoriness or chromosome translocations into wild populations."}
{"id": "PMID:1085662", "title": "A simplified membrane technique for feeding blackflies (Diptera: Simuliidae) on blood in the laboratory.", "content": "In order to overcome the difficulties of colonizing Simuliidae in the laboratory, a membrane system has been developed which was successfully used to feed Simulium vittatum and S. verecundum on out-dated human whole blood. Thirty-seven (41.1%) of 90 S. vittatum and 4 (8.6%) of 46 S. verecundum took blood meals in 6 trials. The membrane system was also shown to be more effective and consistent in providing blood meals compared with the use of laboratory animals.", "contents": "A simplified membrane technique for feeding blackflies (Diptera: Simuliidae) on blood in the laboratory. In order to overcome the difficulties of colonizing Simuliidae in the laboratory, a membrane system has been developed which was successfully used to feed Simulium vittatum and S. verecundum on out-dated human whole blood. Thirty-seven (41.1%) of 90 S. vittatum and 4 (8.6%) of 46 S. verecundum took blood meals in 6 trials. The membrane system was also shown to be more effective and consistent in providing blood meals compared with the use of laboratory animals."}
{"id": "PMID:1085663", "title": "A new technique for the determination of microfilarial densities in onchocerciasis.", "content": "Precise measurement of the parasite load in helminthic infections makes it possible to correlate morbidity, the individual response to treatment, and the best control methods. A membrane filtration technique was recently developed for quantifying Wuchereria bancrofti microfilaraemia. The present paper describes its application to onchocerciasis in a study on 107 patients in northern Togo. The technique is cheap and simple to apply under field conditions, and is extremely efficient at recovering microfilariae from skin snips.", "contents": "A new technique for the determination of microfilarial densities in onchocerciasis. Precise measurement of the parasite load in helminthic infections makes it possible to correlate morbidity, the individual response to treatment, and the best control methods. A membrane filtration technique was recently developed for quantifying Wuchereria bancrofti microfilaraemia. The present paper describes its application to onchocerciasis in a study on 107 patients in northern Togo. The technique is cheap and simple to apply under field conditions, and is extremely efficient at recovering microfilariae from skin snips."}
{"id": "PMID:1085664", "title": "Relationship of serum copper and zinc to human hypertension in Nigerians.", "content": "Serum copper and zinc levels were determined by atomic absorption spectroscopy in 48 hypertensive Nigerians and in 37 normotensive controls. Serum copper and total cholesterol levels were significantly increased in the hypertensive patients, but their serum zinc levels did not differ from those of the controls.", "contents": "Relationship of serum copper and zinc to human hypertension in Nigerians. Serum copper and zinc levels were determined by atomic absorption spectroscopy in 48 hypertensive Nigerians and in 37 normotensive controls. Serum copper and total cholesterol levels were significantly increased in the hypertensive patients, but their serum zinc levels did not differ from those of the controls."}
{"id": "PMID:1085665", "title": "The measles eradication programme in the German Democratic Republic.", "content": "After 3 years of intensive preparations the Ministry of Health of the German Democratic Republic started a national measles eradication programme in 1967. Vaccination was at first voluntary, but became compulsory in 1970. Through a vaccination campaign using Leningrad-16/SSW attenuated live vaccine, the disease was virtually eliminated throughout the country by 1972. In 1973 and 1974 only sporadic cases of measles were observed. The number of vaccination failures was negligible. Combined immunization against measles and poliomyelitis provoked no unusual reactions, and the difference between the seroconversion rates following combined or separate immunization was not statistically significant.", "contents": "The measles eradication programme in the German Democratic Republic. After 3 years of intensive preparations the Ministry of Health of the German Democratic Republic started a national measles eradication programme in 1967. Vaccination was at first voluntary, but became compulsory in 1970. Through a vaccination campaign using Leningrad-16/SSW attenuated live vaccine, the disease was virtually eliminated throughout the country by 1972. In 1973 and 1974 only sporadic cases of measles were observed. The number of vaccination failures was negligible. Combined immunization against measles and poliomyelitis provoked no unusual reactions, and the difference between the seroconversion rates following combined or separate immunization was not statistically significant."}
{"id": "PMID:1085666", "title": "Ricettsioses studies. 2. Natural foci of rickettsioses in east Slovakia.", "content": "Natural foci of Q fever and of spotted fever group rickettsiae in the Kosice district of east Slovakia are described and discussed. It was established that the natural focus of Q fever was a secondary one. Cattle were observed to be the main source of human infection and a high proportion of synanthropic rodents was found to be infested with Coxiella burnetii. The natural focus of spotted fever group rickettsiae was shown to be of a primary character, such rickettsiae circulating among ticks and small mammals. It is suggested that natural foci of spotted fever group rickettsiae in east Slovakia may be connected with the natural distribution of Dermacentor ticks.", "contents": "Ricettsioses studies. 2. Natural foci of rickettsioses in east Slovakia. Natural foci of Q fever and of spotted fever group rickettsiae in the Kosice district of east Slovakia are described and discussed. It was established that the natural focus of Q fever was a secondary one. Cattle were observed to be the main source of human infection and a high proportion of synanthropic rodents was found to be infested with Coxiella burnetii. The natural focus of spotted fever group rickettsiae was shown to be of a primary character, such rickettsiae circulating among ticks and small mammals. It is suggested that natural foci of spotted fever group rickettsiae in east Slovakia may be connected with the natural distribution of Dermacentor ticks."}
{"id": "PMID:1085667", "title": "Enzyme immunoassays in diagnostic medicine. Theory and practice.", "content": "Serological methods are playing an increasingly important role in the diagnosis and epidemiological assessment of diseases. Simple, inexpensive methods for large-scale application are urgently needed. The enzyme immunoassay methods developed recently and reviewed here hold great promise for application in a wide variety of conditions. Under laboratory conditions they can be as sensitive as radio-immunoassay, but they can also be adapted as simple field screening procedures. These methods are based on the use of antibodies or antigens that are linked to an insoluble carrier surface. This is then used to \"capture\" the relevant antigen or antibody in the test solution and the complex is detected by means of an enzyme-labelled antibody or antigen. The degradation of the enzyme substrate, measured photometrically, is proportional to the concentration of the unknown \"antibody\" or \"antigen\" in the test solution. The application of these techniques to endocrinology, immunopathology, haematology, microbiology, and parasitology is reviewed.", "contents": "Enzyme immunoassays in diagnostic medicine. Theory and practice. Serological methods are playing an increasingly important role in the diagnosis and epidemiological assessment of diseases. Simple, inexpensive methods for large-scale application are urgently needed. The enzyme immunoassay methods developed recently and reviewed here hold great promise for application in a wide variety of conditions. Under laboratory conditions they can be as sensitive as radio-immunoassay, but they can also be adapted as simple field screening procedures. These methods are based on the use of antibodies or antigens that are linked to an insoluble carrier surface. This is then used to \"capture\" the relevant antigen or antibody in the test solution and the complex is detected by means of an enzyme-labelled antibody or antigen. The degradation of the enzyme substrate, measured photometrically, is proportional to the concentration of the unknown \"antibody\" or \"antigen\" in the test solution. The application of these techniques to endocrinology, immunopathology, haematology, microbiology, and parasitology is reviewed."}
{"id": "PMID:1085668", "title": "Research needs in taeniasis-cysticercosis.", "content": "This Memorandum discusses the epidemiology of taeniasis-cysticercosis, particularly the survival of taeniid eggs in nature, and goes on to consider diagnostic procedures (parasitological and serological), resistance to infection, pathogenesis and clinical pathology, chemotherapy, and the economic and social consequences of infection. Topics requiring further research are listed, and recommendations are made concerning the approach to the problem.", "contents": "Research needs in taeniasis-cysticercosis. This Memorandum discusses the epidemiology of taeniasis-cysticercosis, particularly the survival of taeniid eggs in nature, and goes on to consider diagnostic procedures (parasitological and serological), resistance to infection, pathogenesis and clinical pathology, chemotherapy, and the economic and social consequences of infection. Topics requiring further research are listed, and recommendations are made concerning the approach to the problem."}
{"id": "PMID:1085669", "title": "Haemagglutination-inhibiting antibodies against swine influenza and Hong Kong influenza viruses in swine sera in the USA.", "content": "Previous reports have established that swine in the midwestern states of the USA have a high incidence of classical swine influenza and that swine become infected with Hong Kong-like influenza viruses when these are prevalent in the human population. This investigation was undertaken to estimate, on the basis of 2245 sera collected randomly from swine going to slaughter in the USA during the summer months of 1974, how many of the animals had haemagglutination-inhibiting (HI) antibodies against swine influenza and Hong Kong influenza viruses. Based on HI titres of 20 or greater, our serological survey revealed that swine influenza virus infection was widespread throughout the USA, since 20.45% of the sera tested had positive HI titres. However, serological evidence of infection of swine with Hong Kong-like viruses was present in only 2.63% of the sera tested.", "contents": "Haemagglutination-inhibiting antibodies against swine influenza and Hong Kong influenza viruses in swine sera in the USA. Previous reports have established that swine in the midwestern states of the USA have a high incidence of classical swine influenza and that swine become infected with Hong Kong-like influenza viruses when these are prevalent in the human population. This investigation was undertaken to estimate, on the basis of 2245 sera collected randomly from swine going to slaughter in the USA during the summer months of 1974, how many of the animals had haemagglutination-inhibiting (HI) antibodies against swine influenza and Hong Kong influenza viruses. Based on HI titres of 20 or greater, our serological survey revealed that swine influenza virus infection was widespread throughout the USA, since 20.45% of the sera tested had positive HI titres. However, serological evidence of infection of swine with Hong Kong-like viruses was present in only 2.63% of the sera tested."}
{"id": "PMID:1085671", "title": "Psychosocial aspects of abortion. A review of issues and needed research.", "content": "The literature on psychosocial aspects of abortion is confusing. Individual publications must be interpreted in the context of cultural, religious, and legal constraints obtaining in a particular society at a given time, with due attention to the status and availability of alternatives to abortion that might be chosen by a woman with an \"unwanted\" pregnancy. A review of the literature shows that, where careful pre- and post-abortion assessments are made, the evidence is that psychological benefit commonly results, and serious adverse emotional sequelae are rare. The outcome of refused abortion seems less satisfactory, with regrets and distress frequently occurring. Research on the administration of abortion services suggests that counselling is often of value, that distress is frequently caused by delays in deciding upon and in carrying out abortions, and by unsympathetic attitudes of service providers. The phenomenon of repeated abortion seeking should be seen in the context of the availability and cost of contraception and sterilization. The place of sterilization with abortion requires careful study. A recommendation is made for observational descriptive research on populations of women with potentially unwanted pregnancies in different cultures, with comparisons of management systems and an evaluation of their impact on service users.", "contents": "Psychosocial aspects of abortion. A review of issues and needed research. The literature on psychosocial aspects of abortion is confusing. Individual publications must be interpreted in the context of cultural, religious, and legal constraints obtaining in a particular society at a given time, with due attention to the status and availability of alternatives to abortion that might be chosen by a woman with an \"unwanted\" pregnancy. A review of the literature shows that, where careful pre- and post-abortion assessments are made, the evidence is that psychological benefit commonly results, and serious adverse emotional sequelae are rare. The outcome of refused abortion seems less satisfactory, with regrets and distress frequently occurring. Research on the administration of abortion services suggests that counselling is often of value, that distress is frequently caused by delays in deciding upon and in carrying out abortions, and by unsympathetic attitudes of service providers. The phenomenon of repeated abortion seeking should be seen in the context of the availability and cost of contraception and sterilization. The place of sterilization with abortion requires careful study. A recommendation is made for observational descriptive research on populations of women with potentially unwanted pregnancies in different cultures, with comparisons of management systems and an evaluation of their impact on service users."}
{"id": "PMID:1085677", "title": "Immunologic regulation of spontaneous antibodies to DNA and RNA I. Significance of IgM and IgG antibodies in SLE patients and asymptomatic relatives.", "content": "Nine individuals from four families of patients with systemic lupus erythematosus (SLE) were studied by sucrose density gradient fractionation and filter radioimmunoassay for the presence of 19S IgM and 7S IgG antibodies to DNA, poly rA, and poly rA-poly rU. One individual in each family was totally asymptomatic, and at least one had actively systemic lupus erythematosus (SLE). The results indicate: (1) a correlation between 7S antibody to DNA and RNA and active SLE, and (2) the presence of 19S antibody to RNA in the asymptomatic relatives. These findings suggest that SLE may be a disorder of immunological regulation. The distribution of antibodies between IgM and IgG is closely related to disease severity. the asymptomatic relatives may have a partial regulatory abnormality resulting in the limited production of IgM antibodies to RNA. SLE patients may have a more complex failure of regulation permitting the additional synthesis of IgG antibodies to DNA and RNA.", "contents": "Immunologic regulation of spontaneous antibodies to DNA and RNA I. Significance of IgM and IgG antibodies in SLE patients and asymptomatic relatives. Nine individuals from four families of patients with systemic lupus erythematosus (SLE) were studied by sucrose density gradient fractionation and filter radioimmunoassay for the presence of 19S IgM and 7S IgG antibodies to DNA, poly rA, and poly rA-poly rU. One individual in each family was totally asymptomatic, and at least one had actively systemic lupus erythematosus (SLE). The results indicate: (1) a correlation between 7S antibody to DNA and RNA and active SLE, and (2) the presence of 19S antibody to RNA in the asymptomatic relatives. These findings suggest that SLE may be a disorder of immunological regulation. The distribution of antibodies between IgM and IgG is closely related to disease severity. the asymptomatic relatives may have a partial regulatory abnormality resulting in the limited production of IgM antibodies to RNA. SLE patients may have a more complex failure of regulation permitting the additional synthesis of IgG antibodies to DNA and RNA."}
{"id": "PMID:1085678", "title": "Disease activity in the nephritis of systemic lupus erythematosus in relation to serum complement concentrations. DNA-binding capacity and precipitating anti-DNA antibody.", "content": "Serum C4 and C3 concentration and binding of double-stranded-DNA (ds-DNA) were measured in sera from ninety-nine patients with systemic lupus erythematosus and clinical evidence of nephritis. C3 and C4 concentrations correlated poorly with ds-DNA binding. In sera from fifty-three patients, precipitating antibody was sought using the counterimmunoelectrophoretic technique. Precipitating antibody was detected on at least one occasion in 44% of the patients, and these sera with precipitating antibody showed higher binding of ds-DNA and lower C4 concentrations than those without precipitating antibody. In thirty-two patients, serial assessments of the activity of the renal disease were made using decline or improvement in glomerular filtration rate, degree of proteinuria, oedema and hypertension as indices of \"activity\". All patients were receiving immunosuppressive drugs. Active nephritis was rarely found in patients showing, at that time, a normal serum C4 or normal ds-DNA binding; but a raised ds-DNA binding or lowered serum C4 were found in both active and inactive nephritis. There was no correlation of activity with serum concentrations of C3, or the presence or absence of precipitating antibody. We conclude that measurements of serum-complement concentrations and binding of ds-DNA are of most use in the diagnosis of systemic lupus erythematosus, and that in patients with nephritis and taking immunosuppressive drugs, these tests are of limited use in guiding treatment.", "contents": "Disease activity in the nephritis of systemic lupus erythematosus in relation to serum complement concentrations. DNA-binding capacity and precipitating anti-DNA antibody. Serum C4 and C3 concentration and binding of double-stranded-DNA (ds-DNA) were measured in sera from ninety-nine patients with systemic lupus erythematosus and clinical evidence of nephritis. C3 and C4 concentrations correlated poorly with ds-DNA binding. In sera from fifty-three patients, precipitating antibody was sought using the counterimmunoelectrophoretic technique. Precipitating antibody was detected on at least one occasion in 44% of the patients, and these sera with precipitating antibody showed higher binding of ds-DNA and lower C4 concentrations than those without precipitating antibody. In thirty-two patients, serial assessments of the activity of the renal disease were made using decline or improvement in glomerular filtration rate, degree of proteinuria, oedema and hypertension as indices of \"activity\". All patients were receiving immunosuppressive drugs. Active nephritis was rarely found in patients showing, at that time, a normal serum C4 or normal ds-DNA binding; but a raised ds-DNA binding or lowered serum C4 were found in both active and inactive nephritis. There was no correlation of activity with serum concentrations of C3, or the presence or absence of precipitating antibody. We conclude that measurements of serum-complement concentrations and binding of ds-DNA are of most use in the diagnosis of systemic lupus erythematosus, and that in patients with nephritis and taking immunosuppressive drugs, these tests are of limited use in guiding treatment."}
{"id": "PMID:1085679", "title": "The use of cryopreserved lymphocytes for longitudinal studies of immune function and enumeration of subpopulations.", "content": "The responses of fresh and frozen lymphocytes to mitogens and antigens have been compared using samples collected on five separate occasions from one normal donor. The day-to-day variation seen with the fresh cells was eliminated by the use of frozen cells. Thawed cells from one donor collected on one occasion but studied on five separate occasions and compared to fresh cells on the same days, showed fluctuations from day to day as well, confirming that the day-to-day variation seen is due to technical and not biological phenomena. Cryopreserved cells showed a decrease in responses to specific microbial antigens, a slight shift in the PHA dose-response curve, but no significant difference in responses to Con A or PWM. The relative proportion of lymphocyte subpopulations changed with freezing and thawing. The proportion of T cells increased slightly and the proportion of B cells decreased.", "contents": "The use of cryopreserved lymphocytes for longitudinal studies of immune function and enumeration of subpopulations. The responses of fresh and frozen lymphocytes to mitogens and antigens have been compared using samples collected on five separate occasions from one normal donor. The day-to-day variation seen with the fresh cells was eliminated by the use of frozen cells. Thawed cells from one donor collected on one occasion but studied on five separate occasions and compared to fresh cells on the same days, showed fluctuations from day to day as well, confirming that the day-to-day variation seen is due to technical and not biological phenomena. Cryopreserved cells showed a decrease in responses to specific microbial antigens, a slight shift in the PHA dose-response curve, but no significant difference in responses to Con A or PWM. The relative proportion of lymphocyte subpopulations changed with freezing and thawing. The proportion of T cells increased slightly and the proportion of B cells decreased."}
{"id": "PMID:1085680", "title": "Human lymphocyte subpopulations effect of epinephrine.", "content": "The effect of administration of 0-2 mg of epinephrine (Parke-Davis) on circulatory lymphocytes was investigated in fifteen normal subjects. Blood samples were taken prior to, 10 and 20 min after, subcutaneous injections. Thymus-derived (T) cells were assayed by sheep red blood cell rosettes, bone marrow-derived (B) cells by their complement receptors and immunofluorescent detection of Fc receptors plus surface immunoglobulins. Their percentages prior to injections were 72-2 +/- 1-4, 13-8 +/- 1-1, and 20-3 +/- 1-3. Ten minutes after injections, the absolute concentrations of these cells increased to 164 +/- 14%, 326 +/- 57%, and 272 +/- 45% respectively of the values prior to injection (averages +/- standard errors). Further, when cells with receptors for both sheep red blood cells and complement were assayed by simultaneous rosette technique, they increased from 2-5 +/- 0-4% to 10-5 +/- 1-3% of the lymphocytes. Such changes were also observed in three subjects who had undergone splenectomy more than 2 years previously, but not in four subjects receiving injections of saline instead of epinephrine.", "contents": "Human lymphocyte subpopulations effect of epinephrine. The effect of administration of 0-2 mg of epinephrine (Parke-Davis) on circulatory lymphocytes was investigated in fifteen normal subjects. Blood samples were taken prior to, 10 and 20 min after, subcutaneous injections. Thymus-derived (T) cells were assayed by sheep red blood cell rosettes, bone marrow-derived (B) cells by their complement receptors and immunofluorescent detection of Fc receptors plus surface immunoglobulins. Their percentages prior to injections were 72-2 +/- 1-4, 13-8 +/- 1-1, and 20-3 +/- 1-3. Ten minutes after injections, the absolute concentrations of these cells increased to 164 +/- 14%, 326 +/- 57%, and 272 +/- 45% respectively of the values prior to injection (averages +/- standard errors). Further, when cells with receptors for both sheep red blood cells and complement were assayed by simultaneous rosette technique, they increased from 2-5 +/- 0-4% to 10-5 +/- 1-3% of the lymphocytes. Such changes were also observed in three subjects who had undergone splenectomy more than 2 years previously, but not in four subjects receiving injections of saline instead of epinephrine."}
{"id": "PMID:1085681", "title": "The effect of Levamisole on peripheral blood lymphocyte subpopulations in patients with rheumatoid arthritis and ankylosing spondylitis.", "content": "Although Levamisole, an antihelmintic drug, has shown to have some modulatory effect on the immune response in clinical trials and experimental models, its mode of action remains obscure. In a group of fifteen patients with rheumatoid arthritis and ankylosing spondylitis receiving Levamisole on an intermittant regime, simultaneous determinations of the lymphocyte subpopulations were made prior to Levamisole administration and 3 months thereafter. No significant changes were observed either in the absolute or in the relative number of T- and B-cell populations, while a statistically significant reduction was found in the \"null\" cells. These findings suggest that the immune potentiating effect of Levamisole may at least partially be due to a maturation process of the \"null\" cells.", "contents": "The effect of Levamisole on peripheral blood lymphocyte subpopulations in patients with rheumatoid arthritis and ankylosing spondylitis. Although Levamisole, an antihelmintic drug, has shown to have some modulatory effect on the immune response in clinical trials and experimental models, its mode of action remains obscure. In a group of fifteen patients with rheumatoid arthritis and ankylosing spondylitis receiving Levamisole on an intermittant regime, simultaneous determinations of the lymphocyte subpopulations were made prior to Levamisole administration and 3 months thereafter. No significant changes were observed either in the absolute or in the relative number of T- and B-cell populations, while a statistically significant reduction was found in the \"null\" cells. These findings suggest that the immune potentiating effect of Levamisole may at least partially be due to a maturation process of the \"null\" cells."}
{"id": "PMID:1085682", "title": "Spontaneous rosette formation by lymphocytes. Differential effect of cytochalasin B on thymocytes and thymus-related peripheral blood lymphocytes in the guinea-pig.", "content": "In the present investigation the differences in the capacity of thymocytes (Th) and thymus-related peripheral blood lymphocytes (TBL) of the guinea-pig to form spontaneous rosettes with rabbit erythrocytes (RRBC) were further evaluated. Cytochalasin B (CB) inhibited TBL rosette formation but was largely ineffective with respect to Th. This suggests that membrane modulation and microfilament function are requirements for the formation of TBL but not of Th rosettes. A certain critical RRBC receptor density on the lymphocyte may be necessary for a stable rosette. Our results suggest that different RRBC receptor densities on Th and TBL account for the differences in rosette forming capacity of these two cell types. In native Th, the receptor density may be high enough to warrant stable rosette formation. In TBL, however, active membrane movement and perhaps clustering of receptors may be necessary to achieve the local critical receptor density as prerequisite of a stable rosette.", "contents": "Spontaneous rosette formation by lymphocytes. Differential effect of cytochalasin B on thymocytes and thymus-related peripheral blood lymphocytes in the guinea-pig. In the present investigation the differences in the capacity of thymocytes (Th) and thymus-related peripheral blood lymphocytes (TBL) of the guinea-pig to form spontaneous rosettes with rabbit erythrocytes (RRBC) were further evaluated. Cytochalasin B (CB) inhibited TBL rosette formation but was largely ineffective with respect to Th. This suggests that membrane modulation and microfilament function are requirements for the formation of TBL but not of Th rosettes. A certain critical RRBC receptor density on the lymphocyte may be necessary for a stable rosette. Our results suggest that different RRBC receptor densities on Th and TBL account for the differences in rosette forming capacity of these two cell types. In native Th, the receptor density may be high enough to warrant stable rosette formation. In TBL, however, active membrane movement and perhaps clustering of receptors may be necessary to achieve the local critical receptor density as prerequisite of a stable rosette."}
{"id": "PMID:1085688", "title": "Unusual roentgenographic manifestation of Pneumocystis carinii pneumonia.", "content": "An immunologically deficient patient had two large nodular densities on the chest x-ray film. On autopsy, large solid accumulations and diffuse pneumonitis with Pneumocystis carinii were found. This represents an unusual manifestation of Pneumocystis carinii.", "contents": "Unusual roentgenographic manifestation of Pneumocystis carinii pneumonia. An immunologically deficient patient had two large nodular densities on the chest x-ray film. On autopsy, large solid accumulations and diffuse pneumonitis with Pneumocystis carinii were found. This represents an unusual manifestation of Pneumocystis carinii."}
{"id": "PMID:1085689", "title": "Primary choroid plexus papilloma of the cerebellopontine angle presenting as brain stem tumor in child.", "content": "A case of a primary choroid plexus papilloma of the cerebellopontine angle in an 8-year-old female is presented. The clinical features of progressive cranial nerve palsies and cerebellar signs in the absence of intracranial hypertension initially suggested an intrinsic brain stem lesion. Whereas a radionuclide brain scan demonstrated abnormal uptake in the region of the pons, vertebral angiography and pneumoencephalography were diagnostic of an angle mass. At surgery, the tumor proved to be a choroid plexus papilloma that was totally confined to the cerebellopontine angle. This patient represents the thirteenth reported case of a primary cerebellopontine angle papilloma and the first such case occurring in a child.", "contents": "Primary choroid plexus papilloma of the cerebellopontine angle presenting as brain stem tumor in child. A case of a primary choroid plexus papilloma of the cerebellopontine angle in an 8-year-old female is presented. The clinical features of progressive cranial nerve palsies and cerebellar signs in the absence of intracranial hypertension initially suggested an intrinsic brain stem lesion. Whereas a radionuclide brain scan demonstrated abnormal uptake in the region of the pons, vertebral angiography and pneumoencephalography were diagnostic of an angle mass. At surgery, the tumor proved to be a choroid plexus papilloma that was totally confined to the cerebellopontine angle. This patient represents the thirteenth reported case of a primary cerebellopontine angle papilloma and the first such case occurring in a child."}
{"id": "PMID:1085690", "title": "[Endoscopic diagnosis and directed surgical treatment of the Mallory-Weiss syndrome using cardiatomy].", "content": "18 cases of Mallory-Weiss-syndrome verified endoscopically are reported. The patient group is surveyed with regard to age and sex distribution as well as accompanying diseases responsible for vomiting. 16 patients were treated conservatively, 2 underwent surgery. Diagnosis, indication of surgical management by selective cardiomyotomy and fundal plasty are described in 2 cases.", "contents": "[Endoscopic diagnosis and directed surgical treatment of the Mallory-Weiss syndrome using cardiatomy]. 18 cases of Mallory-Weiss-syndrome verified endoscopically are reported. The patient group is surveyed with regard to age and sex distribution as well as accompanying diseases responsible for vomiting. 16 patients were treated conservatively, 2 underwent surgery. Diagnosis, indication of surgical management by selective cardiomyotomy and fundal plasty are described in 2 cases."}
{"id": "PMID:1085691", "title": "[Massive gastrointestinal hemorrhage following internal drainage of pancreatic pseudocysts].", "content": "In operative treatment of pancreatic pseudocysts by inner drainage there is a risk of massive gastrointestinal bleeding particularly following an anastomosis to the stomach. In 10 patients in whom cystogastrostomy or cystoduodenostomy had been performed elsewhere a second laparotomy was necessary because of acute bleeding. In one patient a cystadenoma of the pancreas had been anastomosed to the duodenum by mistake at the previous operation. The leak of obliteration of the cyst is suggested to be the most important factor in the pathophysiology of bleeding. Inner pseudocyst drainage in a disconnected small bowel loop therefore principally should be performed at the lowest point of the cyst. The indication for an inner cyst drainage, however, must be closely examined since simultaneous pathologic changes in the pancreas often justify a resection to remove the origin of the cyst and, further, averting the complications of an inadequate inner pancreatic cyst drainage.", "contents": "[Massive gastrointestinal hemorrhage following internal drainage of pancreatic pseudocysts]. In operative treatment of pancreatic pseudocysts by inner drainage there is a risk of massive gastrointestinal bleeding particularly following an anastomosis to the stomach. In 10 patients in whom cystogastrostomy or cystoduodenostomy had been performed elsewhere a second laparotomy was necessary because of acute bleeding. In one patient a cystadenoma of the pancreas had been anastomosed to the duodenum by mistake at the previous operation. The leak of obliteration of the cyst is suggested to be the most important factor in the pathophysiology of bleeding. Inner pseudocyst drainage in a disconnected small bowel loop therefore principally should be performed at the lowest point of the cyst. The indication for an inner cyst drainage, however, must be closely examined since simultaneous pathologic changes in the pancreas often justify a resection to remove the origin of the cyst and, further, averting the complications of an inadequate inner pancreatic cyst drainage."}
{"id": "PMID:1085692", "title": "[Field study on the therapeutic value of trasylol in traumatic shock].", "content": "A report of the results of a multicenter study including 4686 patients concerning the therapeutic value of Trasylol (Aprotinin) in traumatic shock. The group treated additionally with Trasylol showed, beside a reduced overall mortality, a statistically significant reduction of mortality in those treated within the first 30 min after the injury. Surgery performed in the posttraumatic stage showed a lower mortality in the Trasylol group. The importance of a time factor in shock treatment and the effects of Trasylol in traumatic shock are emphasized.", "contents": "[Field study on the therapeutic value of trasylol in traumatic shock]. A report of the results of a multicenter study including 4686 patients concerning the therapeutic value of Trasylol (Aprotinin) in traumatic shock. The group treated additionally with Trasylol showed, beside a reduced overall mortality, a statistically significant reduction of mortality in those treated within the first 30 min after the injury. Surgery performed in the posttraumatic stage showed a lower mortality in the Trasylol group. The importance of a time factor in shock treatment and the effects of Trasylol in traumatic shock are emphasized."}
{"id": "PMID:1085697", "title": "Secondary cytotoxic allograft responses in vitro. II. Differentiation of memory T cells into cytotoxic T lymphocytes in the absence of cell proliferation.", "content": "Murine cytotoxic T lymphocytes (CTL) were induced in \"one-way\" mixed lymphocyte cultures and their physical characteristics investigated by velocity sedimentation at 1 X g. The in vitro differentiation of progenitors of CTL into primary and secondary CTL was paralleled by characteristic changes in the size of the responder cells. Fractionated cells enriched for primary blast CTL reverted into clonally restricted \"nonlytic\" secondary T lymphocytes. Upon antigenic reexposure, these lymphocytes differentiated into secondary CTL within 18-32 h. This took place in the absence of cell proliferation and could be triggered by UV light irradiated allogeneic stimulator cells. It is suggested the different characteristics for the induction of either a primary or secondary cytotoxic T cell response reflect qualitative differences between unprimed T cells and memory T lymphocytes.", "contents": "Secondary cytotoxic allograft responses in vitro. II. Differentiation of memory T cells into cytotoxic T lymphocytes in the absence of cell proliferation. Murine cytotoxic T lymphocytes (CTL) were induced in \"one-way\" mixed lymphocyte cultures and their physical characteristics investigated by velocity sedimentation at 1 X g. The in vitro differentiation of progenitors of CTL into primary and secondary CTL was paralleled by characteristic changes in the size of the responder cells. Fractionated cells enriched for primary blast CTL reverted into clonally restricted \"nonlytic\" secondary T lymphocytes. Upon antigenic reexposure, these lymphocytes differentiated into secondary CTL within 18-32 h. This took place in the absence of cell proliferation and could be triggered by UV light irradiated allogeneic stimulator cells. It is suggested the different characteristics for the induction of either a primary or secondary cytotoxic T cell response reflect qualitative differences between unprimed T cells and memory T lymphocytes."}
{"id": "PMID:1085698", "title": "Rapid recovery of antigen-binding receptors on chicken B cells following anti-Ig serum treatment.", "content": "Rosette-forming cells (RFC) from the peripheral blood of sheep red blood cell (SRBC)-immunized chickens were characterized as B cells which manifest antigen-binding receptors but no antibody secretion. Lymphocytes were pretreated at 0 degrees C with anti-Ig serum, washed, incubated further at various temperatures and harvested for the rosette-forming cells (RFC) assay. Anti-Ig treatment blocked all RFC formation and providing that the cells were maintained at less than 10 degrees C regeneration did not occur up to 6 h. Complete recovery of RFC formation occurred within 10 min at 37 degrees C following treatment with low but not with high concentrations of anti-Ig serum. However, recovery from inhibition by high-dose anti-Ig treatment was achieved by including chicken IgG in the incubation medium. The recovery of receptors was reduced following \"sandwich\" treatment of lymphocytes. When cells were treated with anti-Ig antibody, washed and exposed to various drug inhibitors of \"capping\" the regeneration of RFC was prevented; in contrast, inhibitors of protein synthesis were ineffective. The results were interpreted in terms of dissociation of anti-Ig antibodies from the Ig receptor at an early stage of the ligand-induced receptor redistribution process.", "contents": "Rapid recovery of antigen-binding receptors on chicken B cells following anti-Ig serum treatment. Rosette-forming cells (RFC) from the peripheral blood of sheep red blood cell (SRBC)-immunized chickens were characterized as B cells which manifest antigen-binding receptors but no antibody secretion. Lymphocytes were pretreated at 0 degrees C with anti-Ig serum, washed, incubated further at various temperatures and harvested for the rosette-forming cells (RFC) assay. Anti-Ig treatment blocked all RFC formation and providing that the cells were maintained at less than 10 degrees C regeneration did not occur up to 6 h. Complete recovery of RFC formation occurred within 10 min at 37 degrees C following treatment with low but not with high concentrations of anti-Ig serum. However, recovery from inhibition by high-dose anti-Ig treatment was achieved by including chicken IgG in the incubation medium. The recovery of receptors was reduced following \"sandwich\" treatment of lymphocytes. When cells were treated with anti-Ig antibody, washed and exposed to various drug inhibitors of \"capping\" the regeneration of RFC was prevented; in contrast, inhibitors of protein synthesis were ineffective. The results were interpreted in terms of dissociation of anti-Ig antibodies from the Ig receptor at an early stage of the ligand-induced receptor redistribution process."}
{"id": "PMID:1085699", "title": "Requirement for accessory cells in the antibody response to T cell-independent antigens in vitro.", "content": "The antibody responses of mouse spleen cells in vitro to three thymus-independent (TI) antigens namely, polymeric flagellin (POL) of Salmonella adelaide, DNP-Ficoll and \"soluble\" sheep erythrocyte (SRBC) antigen, were found to be dependent on adherent accessory cells (A cells) but to a lesser degree than the response to intact SRBC. Evidence for this comes from selective depletion of A cells from spleen cells and reconstitution with A cell-rich populations. Thus, depletion of A cells by adherence on glass resulted in abolition of the response to SRBC leaving the response to POL intact. More thorough removal of A cells by treatment with carbonyl iron powder was required for appreciable reduction of the responses to POL, DNP-Ficoll and \"soluble\" SRBC. This reduction in responsiveness was not due to poor cell survival after A cell depletion or to the loss of immunocompetent cells since 1) the recoveries of viable cells in all cultures were similar; 2) the contents of theta- and Ig-bearing cells and tritium-labeled POL-binding cells were unaltered after carbonyl iron treatment, and 3) responsiveness was fully restored by the addition of irradiated and anti-theta-treated A cells from the peritoneal cavity or the spleen. Hence, the hitherto A cell independence of TI antigens on which some theories of B cell activation are based is a result of inadequate depletion procedures, and the minimal model for B cell activation must take into account two cell types: B cells and A cells.", "contents": "Requirement for accessory cells in the antibody response to T cell-independent antigens in vitro. The antibody responses of mouse spleen cells in vitro to three thymus-independent (TI) antigens namely, polymeric flagellin (POL) of Salmonella adelaide, DNP-Ficoll and \"soluble\" sheep erythrocyte (SRBC) antigen, were found to be dependent on adherent accessory cells (A cells) but to a lesser degree than the response to intact SRBC. Evidence for this comes from selective depletion of A cells from spleen cells and reconstitution with A cell-rich populations. Thus, depletion of A cells by adherence on glass resulted in abolition of the response to SRBC leaving the response to POL intact. More thorough removal of A cells by treatment with carbonyl iron powder was required for appreciable reduction of the responses to POL, DNP-Ficoll and \"soluble\" SRBC. This reduction in responsiveness was not due to poor cell survival after A cell depletion or to the loss of immunocompetent cells since 1) the recoveries of viable cells in all cultures were similar; 2) the contents of theta- and Ig-bearing cells and tritium-labeled POL-binding cells were unaltered after carbonyl iron treatment, and 3) responsiveness was fully restored by the addition of irradiated and anti-theta-treated A cells from the peritoneal cavity or the spleen. Hence, the hitherto A cell independence of TI antigens on which some theories of B cell activation are based is a result of inadequate depletion procedures, and the minimal model for B cell activation must take into account two cell types: B cells and A cells."}
{"id": "PMID:1085700", "title": "IgG response in vitro. I. The requirement for an intermediate responsive cell type.", "content": "We have studied a secondary IgG anti-dinitrophenyl response in dissociated mouse spleen cell cultures which is comparable in magnitude to the responses detected after adoptive transfer. The data indicate that the responsive precursor cell is not a recirculating memory cell but is an intermediate B cell type which appears in vivo after antigen challenge of primed mice. Both antigen and T helper cells are required for in vitro stimulation of the intermediate cell type into the division and maturation steps leading to IgG antibody secretion. The buoyant density of precursor cells has been analyzed by bovine serum albumin density gradient centrifugation, and is consistent with their recent origin by division from memory cells.", "contents": "IgG response in vitro. I. The requirement for an intermediate responsive cell type. We have studied a secondary IgG anti-dinitrophenyl response in dissociated mouse spleen cell cultures which is comparable in magnitude to the responses detected after adoptive transfer. The data indicate that the responsive precursor cell is not a recirculating memory cell but is an intermediate B cell type which appears in vivo after antigen challenge of primed mice. Both antigen and T helper cells are required for in vitro stimulation of the intermediate cell type into the division and maturation steps leading to IgG antibody secretion. The buoyant density of precursor cells has been analyzed by bovine serum albumin density gradient centrifugation, and is consistent with their recent origin by division from memory cells."}
{"id": "PMID:1085701", "title": "Detection of anti-idiotype reactive cells in the response to phosphorylcholine.", "content": "Upon immunization with phosphorylcholine (PC), BALB/c mice produce antibodies bearing mainly one idiotype which is identical to that of the TEPC 15 PC-binding myeloma protein. By coating sheep red cells (SRC) with TEPC 15 it was possible to detect anti-idiotypic plaques during the course of an immune response to PC. These plaques are detectable only after the anti-PC response has reached a peak and begun to decline. They are specific, since addition of free TEPC 15 and not MOPC 315 prevented their detection. It is suggested that such anti-idiotypic reactive cells might regulate the response to PC.", "contents": "Detection of anti-idiotype reactive cells in the response to phosphorylcholine. Upon immunization with phosphorylcholine (PC), BALB/c mice produce antibodies bearing mainly one idiotype which is identical to that of the TEPC 15 PC-binding myeloma protein. By coating sheep red cells (SRC) with TEPC 15 it was possible to detect anti-idiotypic plaques during the course of an immune response to PC. These plaques are detectable only after the anti-PC response has reached a peak and begun to decline. They are specific, since addition of free TEPC 15 and not MOPC 315 prevented their detection. It is suggested that such anti-idiotypic reactive cells might regulate the response to PC."}
{"id": "PMID:1085702", "title": "Studies on the lymphocytes of sheep. II. Some properties of cells in various compartments of the recirculating lymphocyte pool.", "content": "The proportion of cells bearing surface immunoglobulin (Ig) and the normal lymphocyte transfer (NLT) reactivity of mononuclear cells obtained from the blood, afferent lymph, and efferent lymph from both somatic and intestinal nodes of sheep were measured. The proportion of Ig-positive lymphocytes was approximately 30% in blood and efferent lymph, but only about 10% in afferent lymph. The proportion of unlabeled lymphocytes (T cells) was about the same in all the preparations, as afferent lymph contains 15--20% macrophages. Despite this apparent uniformity in the T cell content, the NLT reactivity of efferent lymph was 3 to 5 times higher than that of blood or afferent lymph. No humoral factors could be detected by incubating cells in the various plasmas in vitro before injection. No inhibitory cells could be detected when mixtures of cell populations were used to initiate lesions. We suggest that lymphocytes in efferent lymph are more reactive than others because they are \"groomed\" by macrophages during their recirculatory passage through the substance of the node.", "contents": "Studies on the lymphocytes of sheep. II. Some properties of cells in various compartments of the recirculating lymphocyte pool. The proportion of cells bearing surface immunoglobulin (Ig) and the normal lymphocyte transfer (NLT) reactivity of mononuclear cells obtained from the blood, afferent lymph, and efferent lymph from both somatic and intestinal nodes of sheep were measured. The proportion of Ig-positive lymphocytes was approximately 30% in blood and efferent lymph, but only about 10% in afferent lymph. The proportion of unlabeled lymphocytes (T cells) was about the same in all the preparations, as afferent lymph contains 15--20% macrophages. Despite this apparent uniformity in the T cell content, the NLT reactivity of efferent lymph was 3 to 5 times higher than that of blood or afferent lymph. No humoral factors could be detected by incubating cells in the various plasmas in vitro before injection. No inhibitory cells could be detected when mixtures of cell populations were used to initiate lesions. We suggest that lymphocytes in efferent lymph are more reactive than others because they are \"groomed\" by macrophages during their recirculatory passage through the substance of the node."}
{"id": "PMID:1085703", "title": "Fc-rosetting and nonrosetting T cells are killer cells.", "content": "A fraction of T cells activated to H-2 determinants in vivo (ATO) express Fc receptors (FcR) on the cell surface. Those FcR can be detected by antibody coated red blood cells (EA rosettes). Nonrosetting and EA rosetting ATC could be obtained in highly enriched form (more than 99% pure) by separation by velocity sedimentation at unit gravity. Separately tested, both fractions of ATC showed killer cell activity against specific target cells. Possible explanations why only one population of T killer cells expressed FcR are discussed.", "contents": "Fc-rosetting and nonrosetting T cells are killer cells. A fraction of T cells activated to H-2 determinants in vivo (ATO) express Fc receptors (FcR) on the cell surface. Those FcR can be detected by antibody coated red blood cells (EA rosettes). Nonrosetting and EA rosetting ATC could be obtained in highly enriched form (more than 99% pure) by separation by velocity sedimentation at unit gravity. Separately tested, both fractions of ATC showed killer cell activity against specific target cells. Possible explanations why only one population of T killer cells expressed FcR are discussed."}
{"id": "PMID:1085704", "title": "Immature T lineage lymphocytes in athymic mice. Presence of TL, lifespan and homeostatic regulation.", "content": "Lymphocytes belonging to the T lineage were described in athymic nude mice. We showed previously that they were distinguished from usual peripheral lymphocytes by their low density of theta antigen, their slow electrophoretic mobility and their absence of recirculation through the thoracic duct. We now report that they also express the TL antigen, have a life span of 1 to 2 days, are produced in the bone marrow and are under the homeostatic influence of the thymus. Indeed they appear rapidly in surgically T-deprived mice and their production is blocked in both surgically or congenitally athymic mice receiving a thymus graft. This homeostatic control may be mediated via a humoral factor. Cells with similar characteristics are present in the thymus at early stages of embryogenesis. The characteristics of these T lineage lymphocytes strongly suggest that they may represent \"pre-thymocytes\", i.e. cells already committed to the T pathway independently of thymic influence but needing the thymus microenvironment to differentiate further.", "contents": "Immature T lineage lymphocytes in athymic mice. Presence of TL, lifespan and homeostatic regulation. Lymphocytes belonging to the T lineage were described in athymic nude mice. We showed previously that they were distinguished from usual peripheral lymphocytes by their low density of theta antigen, their slow electrophoretic mobility and their absence of recirculation through the thoracic duct. We now report that they also express the TL antigen, have a life span of 1 to 2 days, are produced in the bone marrow and are under the homeostatic influence of the thymus. Indeed they appear rapidly in surgically T-deprived mice and their production is blocked in both surgically or congenitally athymic mice receiving a thymus graft. This homeostatic control may be mediated via a humoral factor. Cells with similar characteristics are present in the thymus at early stages of embryogenesis. The characteristics of these T lineage lymphocytes strongly suggest that they may represent \"pre-thymocytes\", i.e. cells already committed to the T pathway independently of thymic influence but needing the thymus microenvironment to differentiate further."}
{"id": "PMID:1085705", "title": "Interference with histamine and imidazole acetic acid metabolism by salicylates: a possible contribution to salicylate analgesic activity?", "content": "In man, rats and mice, the urinary excretion of the histamine and L-histidine metabolite, imidazole acetic acid, is increased and that of the conjugated metabolite, ribosylimidazole acetic acid, decreased by small doses of salicylates. In contrast to salicylates, other non-salicylate anti-inflammatory drugs, indomethacin, phenylbutazone, phenacetin and acetaminophen do not influence the excretion of the urinary metabolites of histamine and L-histidine. Since imidazole acetic acid is reported to have analgesic and narcotic activity, there is the inference that the analgesic properties of salicylate might be due in part to interference in imidazole acetic acid metabolism.", "contents": "Interference with histamine and imidazole acetic acid metabolism by salicylates: a possible contribution to salicylate analgesic activity? In man, rats and mice, the urinary excretion of the histamine and L-histidine metabolite, imidazole acetic acid, is increased and that of the conjugated metabolite, ribosylimidazole acetic acid, decreased by small doses of salicylates. In contrast to salicylates, other non-salicylate anti-inflammatory drugs, indomethacin, phenylbutazone, phenacetin and acetaminophen do not influence the excretion of the urinary metabolites of histamine and L-histidine. Since imidazole acetic acid is reported to have analgesic and narcotic activity, there is the inference that the analgesic properties of salicylate might be due in part to interference in imidazole acetic acid metabolism."}
{"id": "PMID:1085706", "title": "Ultrastructural identification of human tonsil T-lymphocytes by peroxidase-conjugated anti-HTLA serum.", "content": "A horse anti-serum rendered specific for human T-lymphocytes was conjugated with peroxidase and used for ultrastructural identification of human tonsil T-lymphocytes. With T- and B-enriched suspensions, virtually all T-lymphocytes were labelled with Po-anti-HTLA, whereas no B-cells were stained with this conjugate. The labelling HTLA conjugate confirm the ultrastructural characteristics of thymus-dependant cells.", "contents": "Ultrastructural identification of human tonsil T-lymphocytes by peroxidase-conjugated anti-HTLA serum. A horse anti-serum rendered specific for human T-lymphocytes was conjugated with peroxidase and used for ultrastructural identification of human tonsil T-lymphocytes. With T- and B-enriched suspensions, virtually all T-lymphocytes were labelled with Po-anti-HTLA, whereas no B-cells were stained with this conjugate. The labelling HTLA conjugate confirm the ultrastructural characteristics of thymus-dependant cells."}
{"id": "PMID:1085708", "title": "Independance of glucagon and insulin handling by the isolated perfused dog kidney.", "content": "The effect of raising arterial plasma glucagon concentrations on kidney glucagon uptake was investigated using an isolated dog kidney perfused with whole blood. In addition, the effect of insulin on the magnitude of glucagon uptake by the kidney was studied at various glucagon concentrations. Renal vein plasma glucagon (V) has been found to be proportional to renal artery plasma glucagon (A). V and A were highly significantly correlated. In the absence of exogenous insulin infusion, V equalled 0.733 +/- 0.034 A, while in the presence of insulin V equalled 0.747 +/- 0.015 A. When kidney glucagon uptake was measured directly it increased as a function of arterial plasma glucagon. The calculated regression lines were similar in the presence and in the absence of insulin. The mean clearance rate of glucagon by the kidney was similar at low, medium or high concentrations of glucagon and was not affected by the presence of insulin at a mean concentration of 335.7 +/- 15.7 muU/ml. At this concentration of insulin, kidney insulin uptake was not affected by glucagon at concentrations ranging from 32 to 1600 pg/ml. Comparison of kidney glucagon uptake at similar arterial plasma glucagon concentrations, but with different renal plasma flows, indicated that kidney glucagon uptake is more dependant on arterial plasma glucagon concentration than on the quantity of glucagon entering the kidney per minute. It is concluded that: 1) kidney glucagon uptake increases as a function of arterial plasma glucagon concentration; 2) the clearance rate of glucagon is similar at low, medium or high arterial concentrations of glucagon; 3) at concentration of 300-350 muU/ml, insulin does not affect kidney glucagon uptake, and 4) at concentrations of glucagon up to 1600 pg/ml, renal insulin uptake is not affected by glucagon. These studies indicate that insulin and glucagon are handled independantly by the kidney of the dog.", "contents": "Independance of glucagon and insulin handling by the isolated perfused dog kidney. The effect of raising arterial plasma glucagon concentrations on kidney glucagon uptake was investigated using an isolated dog kidney perfused with whole blood. In addition, the effect of insulin on the magnitude of glucagon uptake by the kidney was studied at various glucagon concentrations. Renal vein plasma glucagon (V) has been found to be proportional to renal artery plasma glucagon (A). V and A were highly significantly correlated. In the absence of exogenous insulin infusion, V equalled 0.733 +/- 0.034 A, while in the presence of insulin V equalled 0.747 +/- 0.015 A. When kidney glucagon uptake was measured directly it increased as a function of arterial plasma glucagon. The calculated regression lines were similar in the presence and in the absence of insulin. The mean clearance rate of glucagon by the kidney was similar at low, medium or high concentrations of glucagon and was not affected by the presence of insulin at a mean concentration of 335.7 +/- 15.7 muU/ml. At this concentration of insulin, kidney insulin uptake was not affected by glucagon at concentrations ranging from 32 to 1600 pg/ml. Comparison of kidney glucagon uptake at similar arterial plasma glucagon concentrations, but with different renal plasma flows, indicated that kidney glucagon uptake is more dependant on arterial plasma glucagon concentration than on the quantity of glucagon entering the kidney per minute. It is concluded that: 1) kidney glucagon uptake increases as a function of arterial plasma glucagon concentration; 2) the clearance rate of glucagon is similar at low, medium or high arterial concentrations of glucagon; 3) at concentration of 300-350 muU/ml, insulin does not affect kidney glucagon uptake, and 4) at concentrations of glucagon up to 1600 pg/ml, renal insulin uptake is not affected by glucagon. These studies indicate that insulin and glucagon are handled independantly by the kidney of the dog."}
{"id": "PMID:1085709", "title": "Initial stages of differentiation of thymocytes in the plasma membrane.", "content": "There is some evidence in the plasma membrane after binding of molecules triggering differentiation processes that different membrane areas exist possessing different functions. We have tried to isolate these different membrane areas, using a type of affinity chromatography on Con A-Sepharose. Our results from binding sites, affinity constant and fatty acid composition seem to reinforce the evidence that heterogeneous plasma membrane areas exist having different functions in the proliferation and differentiation process of thymocytes after triggering with Con A.", "contents": "Initial stages of differentiation of thymocytes in the plasma membrane. There is some evidence in the plasma membrane after binding of molecules triggering differentiation processes that different membrane areas exist possessing different functions. We have tried to isolate these different membrane areas, using a type of affinity chromatography on Con A-Sepharose. Our results from binding sites, affinity constant and fatty acid composition seem to reinforce the evidence that heterogeneous plasma membrane areas exist having different functions in the proliferation and differentiation process of thymocytes after triggering with Con A."}
{"id": "PMID:1085710", "title": "Detection of T-precursor cells in human bone marrow and foetal liver.", "content": "Human lymphoid cells that have been incubated with conditioned medium from confluent monolayers of human thymic epithelium (HTCM) show an increase in cells forming rosettes with sheep erythrocytes at 4 degrees C (E-rosettes). Those cells demonstrating this in vitro conversion have been interpreted to be T-cell precursors. Separation of human bone marrow cells on discontinuous bovine serum albumin (BSA) gradients, and on a single-step 23% BSA gradient showed enrichment of these T-precursor cells, not only in bone marrow, but also in human foetal liver cells. Bone marrow precursor cells from a patient with Wiskott-Aldrich syndrome (T-cell deficiency) showed a normal in vitro response to HTCM, but no response was seen in cells from a patient with severe combined immunodeficiency disease.", "contents": "Detection of T-precursor cells in human bone marrow and foetal liver. Human lymphoid cells that have been incubated with conditioned medium from confluent monolayers of human thymic epithelium (HTCM) show an increase in cells forming rosettes with sheep erythrocytes at 4 degrees C (E-rosettes). Those cells demonstrating this in vitro conversion have been interpreted to be T-cell precursors. Separation of human bone marrow cells on discontinuous bovine serum albumin (BSA) gradients, and on a single-step 23% BSA gradient showed enrichment of these T-precursor cells, not only in bone marrow, but also in human foetal liver cells. Bone marrow precursor cells from a patient with Wiskott-Aldrich syndrome (T-cell deficiency) showed a normal in vitro response to HTCM, but no response was seen in cells from a patient with severe combined immunodeficiency disease."}
{"id": "PMID:1085711", "title": "The importance of clostridia in experimental intestinal strangulation.", "content": "Hemorrhagic and ischemic strangulation of closed intestinal segments was sutdied in germ-free rats individually monocontaminated with one of eight separate species of clostridia. Ischemic strangulation was found to be as deadly as hemorrhagic strangulation in the presence of clostridia. Some clostridia were very toxic whereas others were relatively innocuous. The clostridia, ranked in the order of decreasing lethality, were: Clostridium perfringens type A, C. septicum, C. histolyticum, C. haemolyticum, C. bifermantans, C. sporogenes, C. tertium, and C. novyi type A. All species were readily established in the gastrointestinal tract of the nonoperated germ-free rat. The clostridia appear to be one of the most potentially lethal groups of organisms commonly found in the intestinal tract of human beings and animals.", "contents": "The importance of clostridia in experimental intestinal strangulation. Hemorrhagic and ischemic strangulation of closed intestinal segments was sutdied in germ-free rats individually monocontaminated with one of eight separate species of clostridia. Ischemic strangulation was found to be as deadly as hemorrhagic strangulation in the presence of clostridia. Some clostridia were very toxic whereas others were relatively innocuous. The clostridia, ranked in the order of decreasing lethality, were: Clostridium perfringens type A, C. septicum, C. histolyticum, C. haemolyticum, C. bifermantans, C. sporogenes, C. tertium, and C. novyi type A. All species were readily established in the gastrointestinal tract of the nonoperated germ-free rat. The clostridia appear to be one of the most potentially lethal groups of organisms commonly found in the intestinal tract of human beings and animals."}
{"id": "PMID:1085712", "title": "Assessment of the accuracy of double contrast gastroduodenal radiology.", "content": "The report presents an analysis of routine double contrast gastroduodenal radiology based on our experience with 1500 consecutive examinations. The value of this technique is illustrated by the demonstration of subtle abnormalities such as erosions, linear ulcers, and ulcer scars which are usually not demonstrated by the standard barium study. Two hundred and twenty-five of these patients have also been examined by endoscopy and radiological errors were found in 7%. This represents a marked improvement over the results obtained with the standard method. In the last 128 patients, a confidence level ranging from 3 (certainty) to 1 (uncertain) was assigned to the radiological diagnosis before endoscopy. It was found that the majority of radiological errors occurred in the few studies given the lowest confidence level. These findings indicate that the double contrast study of the stomach and duodenum can be a very accurate examination and that endoscopy can be applied more selectively to patients with inconclusive double contrast studies.", "contents": "Assessment of the accuracy of double contrast gastroduodenal radiology. The report presents an analysis of routine double contrast gastroduodenal radiology based on our experience with 1500 consecutive examinations. The value of this technique is illustrated by the demonstration of subtle abnormalities such as erosions, linear ulcers, and ulcer scars which are usually not demonstrated by the standard barium study. Two hundred and twenty-five of these patients have also been examined by endoscopy and radiological errors were found in 7%. This represents a marked improvement over the results obtained with the standard method. In the last 128 patients, a confidence level ranging from 3 (certainty) to 1 (uncertain) was assigned to the radiological diagnosis before endoscopy. It was found that the majority of radiological errors occurred in the few studies given the lowest confidence level. These findings indicate that the double contrast study of the stomach and duodenum can be a very accurate examination and that endoscopy can be applied more selectively to patients with inconclusive double contrast studies."}
{"id": "PMID:1085715", "title": "[Membrane receptors of lymphoreticular cells in hyperplastic and neoplastic diseases of the lymphatic system].", "content": "The results of T- and B-cell determinations are described in 105 cases of lymphoreticular and lymphoepithelial neoplasia, and are compared to similar investigations of 582 cases as published in the literature. In addition, T- and B-cell values are determined in blood of 35 healthy individuals, in 12 normal lymph nodes, as well as in hyperplastic conditions of lymph nodes from 30 patients and of tonsils from 85 patients. Cell characterizations are done by immunofluorescence and use of monospecific anti-immunoglobulin antisera (H chain specific), anti-thymus antiserum, as well as by the E-rosette test. While normal blood and normal and hyperplastic tissues show a polyclonal distribution or proliferation of lymphoreticular cells, neoplastic conditions are often characterized by an exuberant, possibly monoclonal proliferation of one cell type. According to this, lymphoreticular neoplasias are immunologically grouped into four main classes: B-cell neoplasias comprising most of the chronic lymphocytic leukemias, well differentiated lymphocytic lymphomas, BURKITT's tumor, follicular lymphoma BRILL-SYMMERS, and hairy cell leukemia. T-cell lymphomas represent a large part of poorly or undifferentiated leukemias of children, poorly differentiated lymphocytic lymphomas, prolymphocytic leukemia, and S\u00e9zary's syndrome. Monocytic neoplasias are malignant histiocytoses and leukemic reticuloendothelioses. A fourth group, which probably is not homogeneous and might be further classified in the future by use of more sophisticated methods, consists of tumors with T- and B-cell lack. Such tumors are histologically classified as Hodgkin's lymphomas, a certain number of histiocytic lymphomas, and mycosis fungoides. The prognostic and pathogenetic implications of a combined morphological and immunological classification of lymphoreticular neoplasias are briefly outlined.", "contents": "[Membrane receptors of lymphoreticular cells in hyperplastic and neoplastic diseases of the lymphatic system]. The results of T- and B-cell determinations are described in 105 cases of lymphoreticular and lymphoepithelial neoplasia, and are compared to similar investigations of 582 cases as published in the literature. In addition, T- and B-cell values are determined in blood of 35 healthy individuals, in 12 normal lymph nodes, as well as in hyperplastic conditions of lymph nodes from 30 patients and of tonsils from 85 patients. Cell characterizations are done by immunofluorescence and use of monospecific anti-immunoglobulin antisera (H chain specific), anti-thymus antiserum, as well as by the E-rosette test. While normal blood and normal and hyperplastic tissues show a polyclonal distribution or proliferation of lymphoreticular cells, neoplastic conditions are often characterized by an exuberant, possibly monoclonal proliferation of one cell type. According to this, lymphoreticular neoplasias are immunologically grouped into four main classes: B-cell neoplasias comprising most of the chronic lymphocytic leukemias, well differentiated lymphocytic lymphomas, BURKITT's tumor, follicular lymphoma BRILL-SYMMERS, and hairy cell leukemia. T-cell lymphomas represent a large part of poorly or undifferentiated leukemias of children, poorly differentiated lymphocytic lymphomas, prolymphocytic leukemia, and S\u00e9zary's syndrome. Monocytic neoplasias are malignant histiocytoses and leukemic reticuloendothelioses. A fourth group, which probably is not homogeneous and might be further classified in the future by use of more sophisticated methods, consists of tumors with T- and B-cell lack. Such tumors are histologically classified as Hodgkin's lymphomas, a certain number of histiocytic lymphomas, and mycosis fungoides. The prognostic and pathogenetic implications of a combined morphological and immunological classification of lymphoreticular neoplasias are briefly outlined."}
{"id": "PMID:1085738", "title": "[The effect of a flufenamic acid containing ointment in various rheumatic diseases. A double-blind study].", "content": "Of 100 patients suffering chiefly from soft tissue rheumatism 48 were treated in a double-blind study with an ointment containing flufenamic acid (Mobilisin) and 52 with a control preparation. The test ointment brought about good to very good results in 43 patients (89,5%) whereas only 26 patients (50%) of the control group showed a therapeutic effect. Either product was tolerated well; no side-effects have been observed in any of the cases.", "contents": "[The effect of a flufenamic acid containing ointment in various rheumatic diseases. A double-blind study]. Of 100 patients suffering chiefly from soft tissue rheumatism 48 were treated in a double-blind study with an ointment containing flufenamic acid (Mobilisin) and 52 with a control preparation. The test ointment brought about good to very good results in 43 patients (89,5%) whereas only 26 patients (50%) of the control group showed a therapeutic effect. Either product was tolerated well; no side-effects have been observed in any of the cases."}
{"id": "PMID:1085739", "title": "[Progress in the diagnosis of cutaneous lymphomas. Enzyme cytochemical and immunocytological studies].", "content": "The term \"\"cutaneous lymphoma'' is used for lymphoreticular proliferative processes which primarily affect the skin. This term includes: mycosis fungoides, Sezary-syndrome and \"\"pagetoid epidermotropic reticulosis''; \"\"malignant reticulosis'', \"\"reticulum cell sarcoma'' and lymphosarcoma; in a wide sense of this term pseudolymphomas (lymphadenosis benigna cutis, benign lymphocytoma), specific skin infiltrates in leukemia and Hodgkin's disease might be included as well.", "contents": "[Progress in the diagnosis of cutaneous lymphomas. Enzyme cytochemical and immunocytological studies]. The term \"\"cutaneous lymphoma'' is used for lymphoreticular proliferative processes which primarily affect the skin. This term includes: mycosis fungoides, Sezary-syndrome and \"\"pagetoid epidermotropic reticulosis''; \"\"malignant reticulosis'', \"\"reticulum cell sarcoma'' and lymphosarcoma; in a wide sense of this term pseudolymphomas (lymphadenosis benigna cutis, benign lymphocytoma), specific skin infiltrates in leukemia and Hodgkin's disease might be included as well."}
{"id": "PMID:1085740", "title": "[Complications of Crohn's disease and their surgical treatment].", "content": "Crohn's disease is characterised by its unknown etiology and pathogenesis, its many complications and its extraordinary disposition of recurrence. The perforation of the small intestine, or of the colon, obstruction and massive bleeding are severe complications, requiring immediate surgical attention. Frequently specific formation of fistulae is indicative of the first sign of the disease. A radical operative treatment--by present results (approximately 70% of cases)--is the method of choice. The tendency to extend surgical procedures to the chronic stage of the disease has been discussed in recent years. Complications observed in patients under study have also been analysed.", "contents": "[Complications of Crohn's disease and their surgical treatment]. Crohn's disease is characterised by its unknown etiology and pathogenesis, its many complications and its extraordinary disposition of recurrence. The perforation of the small intestine, or of the colon, obstruction and massive bleeding are severe complications, requiring immediate surgical attention. Frequently specific formation of fistulae is indicative of the first sign of the disease. A radical operative treatment--by present results (approximately 70% of cases)--is the method of choice. The tendency to extend surgical procedures to the chronic stage of the disease has been discussed in recent years. Complications observed in patients under study have also been analysed."}
{"id": "PMID:1085741", "title": "[Spontaneous cholecysto-gastric fistula with massive gastrointestinal bleeding. Endoscopic diagnosis and concrement extraction].", "content": "A case of spontaneous cholecysto-gastric fistula is reported with penetration of a gallstone into the stomach leading to massive haemorrhage of gastric mucosa. The gallstone was removed endoscopically, the immanent complication of gallstone ileus could be eliminated. Mucosal bleeding was stopped by conservative methods, and thus enough time was won for optimal preparation for the ensuing operation. The problems of preoperative diagnosis are mentioned, also rate and early complications of spontaneous bilio-digestive fistulae and their poor prognosis concerning the increased letality at operation.", "contents": "[Spontaneous cholecysto-gastric fistula with massive gastrointestinal bleeding. Endoscopic diagnosis and concrement extraction]. A case of spontaneous cholecysto-gastric fistula is reported with penetration of a gallstone into the stomach leading to massive haemorrhage of gastric mucosa. The gallstone was removed endoscopically, the immanent complication of gallstone ileus could be eliminated. Mucosal bleeding was stopped by conservative methods, and thus enough time was won for optimal preparation for the ensuing operation. The problems of preoperative diagnosis are mentioned, also rate and early complications of spontaneous bilio-digestive fistulae and their poor prognosis concerning the increased letality at operation."}
{"id": "PMID:1085743", "title": "Differential sensitivity to x-ray of chromosomes of blood T-lymphocytes and B-and T-cell lines.", "content": "Normal T-lymphocytes, B-cell line (CCRF-SB) and T-cell line (CCRF-HSB-2) cells, all diploid in their chromosome constitution, were exposed in vitro to various doses of X-ray and analyzed at their first mitotic division for structural chromosome abnormalities. The irradiation effects were determined also by a viability test of the cells, using trypan blue dye. The irradiated T-cell line (CCRF-HSB-2) showed a remarkably high frequency of chromosome aberrations, including chromosome and chromatid deletions, chromatid exchanges, dicentrics, rings and acentric fragments. On the other hand, the chromosome aberrations observed in the irradiated B-cell line and normal T-lymphocytes consisted mainly of dicentrics, rings, deletions and acentric fragments; the frequency of chromosome and chromatid deletions was low as compared to that of the T-cell line. The cell viability test showed a singificantly higher percent reduction of viable cells at every dose of X-ray in the irradiated T-cell line than in the B-cell line or the normal T-lymphocytes. It is possible that the increased radiosensitivity of the T-cell line is related to the original malignant nature of the cells, which originated from the lymphocytes of a patient with acute lymphoblastic leukemia.", "contents": "Differential sensitivity to x-ray of chromosomes of blood T-lymphocytes and B-and T-cell lines. Normal T-lymphocytes, B-cell line (CCRF-SB) and T-cell line (CCRF-HSB-2) cells, all diploid in their chromosome constitution, were exposed in vitro to various doses of X-ray and analyzed at their first mitotic division for structural chromosome abnormalities. The irradiation effects were determined also by a viability test of the cells, using trypan blue dye. The irradiated T-cell line (CCRF-HSB-2) showed a remarkably high frequency of chromosome aberrations, including chromosome and chromatid deletions, chromatid exchanges, dicentrics, rings and acentric fragments. On the other hand, the chromosome aberrations observed in the irradiated B-cell line and normal T-lymphocytes consisted mainly of dicentrics, rings, deletions and acentric fragments; the frequency of chromosome and chromatid deletions was low as compared to that of the T-cell line. The cell viability test showed a singificantly higher percent reduction of viable cells at every dose of X-ray in the irradiated T-cell line than in the B-cell line or the normal T-lymphocytes. It is possible that the increased radiosensitivity of the T-cell line is related to the original malignant nature of the cells, which originated from the lymphocytes of a patient with acute lymphoblastic leukemia."}
{"id": "PMID:1085744", "title": "[The aetiology of rheumatoid arthritis (author's transl)].", "content": "An ineffective aetiology for rheumatoid arthritis and other connective tissue diseases has been frequently postulated but never proven. Despite the failure to obtain firm evidence of viral infection in most patients with these disorders for several reasons this theory should not be discarded. Firstly several mechanisms have been discovered by which virus infections can persist in lymphocytes and other tissue thereby provoking inflammation without the production of complete readily detectable virus particles. Secondly there are numerous ways in which host resistance to virus can be subverted with the perpetuation of an ineffective or inappropriate immune response. Thirdly the immunopathological features of these diseases are entirely compatible with an infective aetiology. The main problem is likely to prove the difficulty in attributing a primary pathogenetic role to any isolated virus rather than regarding it as a passenger virus which has been non-specifically activated by the disease process. However preoccupation with a viral aetiology should not blind one to other possibilities since many environmental allergens can produce immunopathological disease of highly protean nature.", "contents": "[The aetiology of rheumatoid arthritis (author's transl)]. An ineffective aetiology for rheumatoid arthritis and other connective tissue diseases has been frequently postulated but never proven. Despite the failure to obtain firm evidence of viral infection in most patients with these disorders for several reasons this theory should not be discarded. Firstly several mechanisms have been discovered by which virus infections can persist in lymphocytes and other tissue thereby provoking inflammation without the production of complete readily detectable virus particles. Secondly there are numerous ways in which host resistance to virus can be subverted with the perpetuation of an ineffective or inappropriate immune response. Thirdly the immunopathological features of these diseases are entirely compatible with an infective aetiology. The main problem is likely to prove the difficulty in attributing a primary pathogenetic role to any isolated virus rather than regarding it as a passenger virus which has been non-specifically activated by the disease process. However preoccupation with a viral aetiology should not blind one to other possibilities since many environmental allergens can produce immunopathological disease of highly protean nature."}
{"id": "PMID:1085745", "title": "[Classification of non-Hodgkin's lymphomas on the basis of Morphological and immunological features shared by normal and neoplastic lymphatic cells (author's transl)].", "content": "A review is given of the new Kiel classification of non-Hodgkin's lymphomas (NHL) and of the findings which led to its development. The Kiel classification of NHL is based on morphological and immunological features shared by normal and neoplastic cells. Therefore, the origin, maturation, and reaction of nonneoplastic T- and B-cells together with their immunological, enzyme-cytochemical and morphological features are discussed first. In the T-cell system T-precursor cells, prothymocytes, T1/T2-cells, and T-immunoblasts can be distinguished on the basis of the given features. The B-system is primarily composed of cells of the two antigen-dependent B-cell reactions, the germinal center reaction, and the plasma cell reaction. These cells can be distinguished both morphologically and through demonstration of the complement receptor in frozen sections. We differentiate the following entities of NHL, divided into six groups for better understanding: 1. lymphocytic lymphomas: chronic lymphocytic leukemia (CLL), prolymphocytic leukemia, hairy cell leukemia, mycosis fungoides, and S\u00e9zary syndrome; 2. secretory immunoglobulin-producing lymphomas: plasmacytoma and lymphoplasmacytoid immunocytoma; 3. germinal center cell tumors: centroblastic/centrocytic lymphoma, centrocytic lymphoma, centroblastic lymphoma, Burkitt's lymphoma, and lymphoma of the Burkitt type; 4. lymphoblastic lymphomas: \"convoluted cell\" type and \"unclassified\" type; 5. immunoblastic lymphoma; 6. reticulosarcoma. B-cell characteristics are revealed by more than 80% of the NHL, namely by 97% of the cases of CLL, by all secretory immunoglobulin-producing lymphomas, by all germinal center cell tumors, and by most immunoblastic lymphomas. More than 50% of the B-cell tumors are evidently derived from germinal center cells. The following are T-cell tumors: a few rare forms of CLL, mycosis fungoides S\u00e9zary syndrome, about 50% of the lymphoblastic lymphomas, and a few immunoblastic lymphomas. It could be shown that the cells of lymphoblastic lymphoma, \"convolted cell\" type with strong acid phosphatase reactivity have the same features as fetal thymocytes. So far, we have not observed any cases of true reticulosarcoma as described by Roulet and Rappaporti, i.e. tumors of phagocytic reticulum cells. The morphological features which could be defined for the entities distinguished in the Kiel classification of NHL are so characteristic that most of these entities can now be diagnosed on the basis of their histological and cytological pictures alone.", "contents": "[Classification of non-Hodgkin's lymphomas on the basis of Morphological and immunological features shared by normal and neoplastic lymphatic cells (author's transl)]. A review is given of the new Kiel classification of non-Hodgkin's lymphomas (NHL) and of the findings which led to its development. The Kiel classification of NHL is based on morphological and immunological features shared by normal and neoplastic cells. Therefore, the origin, maturation, and reaction of nonneoplastic T- and B-cells together with their immunological, enzyme-cytochemical and morphological features are discussed first. In the T-cell system T-precursor cells, prothymocytes, T1/T2-cells, and T-immunoblasts can be distinguished on the basis of the given features. The B-system is primarily composed of cells of the two antigen-dependent B-cell reactions, the germinal center reaction, and the plasma cell reaction. These cells can be distinguished both morphologically and through demonstration of the complement receptor in frozen sections. We differentiate the following entities of NHL, divided into six groups for better understanding: 1. lymphocytic lymphomas: chronic lymphocytic leukemia (CLL), prolymphocytic leukemia, hairy cell leukemia, mycosis fungoides, and S\u00e9zary syndrome; 2. secretory immunoglobulin-producing lymphomas: plasmacytoma and lymphoplasmacytoid immunocytoma; 3. germinal center cell tumors: centroblastic/centrocytic lymphoma, centrocytic lymphoma, centroblastic lymphoma, Burkitt's lymphoma, and lymphoma of the Burkitt type; 4. lymphoblastic lymphomas: \"convoluted cell\" type and \"unclassified\" type; 5. immunoblastic lymphoma; 6. reticulosarcoma. B-cell characteristics are revealed by more than 80% of the NHL, namely by 97% of the cases of CLL, by all secretory immunoglobulin-producing lymphomas, by all germinal center cell tumors, and by most immunoblastic lymphomas. More than 50% of the B-cell tumors are evidently derived from germinal center cells. The following are T-cell tumors: a few rare forms of CLL, mycosis fungoides S\u00e9zary syndrome, about 50% of the lymphoblastic lymphomas, and a few immunoblastic lymphomas. It could be shown that the cells of lymphoblastic lymphoma, \"convolted cell\" type with strong acid phosphatase reactivity have the same features as fetal thymocytes. So far, we have not observed any cases of true reticulosarcoma as described by Roulet and Rappaporti, i.e. tumors of phagocytic reticulum cells. The morphological features which could be defined for the entities distinguished in the Kiel classification of NHL are so characteristic that most of these entities can now be diagnosed on the basis of their histological and cytological pictures alone."}
{"id": "PMID:1085748", "title": "Immune interferon production by lymphoid cells: role in the inhibition of herpesviruses.", "content": "Bovine peripheral blood lymphocytes (PBL) obtained from infectious bovine rhinotracheitis (IBR) virus- and tuberculin-immunized animals produced large quantities of interferon within 24 h of in vitro stimulation by IBR and purified protein derivative antigens. Separation of PBL into populations enriched in T lymphocytes or B lymphocyte provided the antigen-specific step for immune interferon production. A 2- to 10-fold increase in interferon occurred when lymphocytes were combined with autologous macrophages. Although macrophages, even if treated with antilymphocyte serum to remove any contaminating lymphocytes, could produce some interferon, the augmented interferon produced by macrophage-lypmhocyte cultures was not dmpocytes. Direct physical contact between macrophages and lymphocytes was required for the production of enhanced levels of interferon. Antigen-antibody complexes of irradiated virus-infected cells in the presence of antibody were as efficient or better at stimulating interferon than was free antigen. Because IBR virus was inhibited by interferon levels stimulated in cultures by IBR antigen, it was suggested that the local production of interferon by immune cells might play a similar role in curtailing virus dissemination in vivo, thus leading to recovery from disease.", "contents": "Immune interferon production by lymphoid cells: role in the inhibition of herpesviruses. Bovine peripheral blood lymphocytes (PBL) obtained from infectious bovine rhinotracheitis (IBR) virus- and tuberculin-immunized animals produced large quantities of interferon within 24 h of in vitro stimulation by IBR and purified protein derivative antigens. Separation of PBL into populations enriched in T lymphocytes or B lymphocyte provided the antigen-specific step for immune interferon production. A 2- to 10-fold increase in interferon occurred when lymphocytes were combined with autologous macrophages. Although macrophages, even if treated with antilymphocyte serum to remove any contaminating lymphocytes, could produce some interferon, the augmented interferon produced by macrophage-lypmhocyte cultures was not dmpocytes. Direct physical contact between macrophages and lymphocytes was required for the production of enhanced levels of interferon. Antigen-antibody complexes of irradiated virus-infected cells in the presence of antibody were as efficient or better at stimulating interferon than was free antigen. Because IBR virus was inhibited by interferon levels stimulated in cultures by IBR antigen, it was suggested that the local production of interferon by immune cells might play a similar role in curtailing virus dissemination in vivo, thus leading to recovery from disease."}
{"id": "PMID:1085749", "title": "Genetic analysis of lymphocyte activation by lipopolysaccharide Endotoxin.", "content": "A genetic study of in vitro lymphocyte blastogenesis by the B-cell mitogen lipopolysaccharide endotoxin has been performed, using both low-responder C3H/HeJ mice and high-responder CBA/J mice. The crossbreeding of these strains produced F1 progeny that were intermediate in responsiveness. Responder types from the backcross of the F1 to the C3H/HeJ strain segregated into intermediate- and low-responder phenotypes, whereas intermediate- and high-responder phenotypes were produced from the backcross of the F1 to the CBA/J strain. The F2 generation consisted of all three responder phenotypes in frequencies that fit the classical Mendelian ratio of 1:2:1, indicating that the mitogenic response to lipopolysaccharide endotoxin in mice is most likely governed by a pair of autosomal co-dominant genes.", "contents": "Genetic analysis of lymphocyte activation by lipopolysaccharide Endotoxin. A genetic study of in vitro lymphocyte blastogenesis by the B-cell mitogen lipopolysaccharide endotoxin has been performed, using both low-responder C3H/HeJ mice and high-responder CBA/J mice. The crossbreeding of these strains produced F1 progeny that were intermediate in responsiveness. Responder types from the backcross of the F1 to the C3H/HeJ strain segregated into intermediate- and low-responder phenotypes, whereas intermediate- and high-responder phenotypes were produced from the backcross of the F1 to the CBA/J strain. The F2 generation consisted of all three responder phenotypes in frequencies that fit the classical Mendelian ratio of 1:2:1, indicating that the mitogenic response to lipopolysaccharide endotoxin in mice is most likely governed by a pair of autosomal co-dominant genes."}
{"id": "PMID:1085750", "title": "Identification and quantitation of capsular antigen in capsulated and noncapsulated strains of Haemophilus influenzae type b by crossed-immunoelectrophoresis.", "content": "Sonicated preparations of capsulated Haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class I variant) and the other noncapsulated (class II variant), and a noncapsulated strain of H. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed antiserum to capsulated H. influenzae type b. Twenty common antigens were present in all four cultures. Two type b-specific antigens were also identified in the three H. influenzae type b cultures when antiserum adsorbed with H. influenzae type d sonicates (AdR) was used. One of these, the type b capsular antigen, cross-reacted with an antigen of Bacillus pumilus. Further adsorption of AdR with the B. pumilus sonicate reduced, but did not eliminate, the antibodies to the type b capsular antigen, although all antibodies to B. pumilus were removed. Sonication sheared the type b capsular antigen, resulting in an increase in its electrophoretic mobility in agarose gel. The capsular antigen from all three H. influenzae type b cultures had the same electrophoretic characteristics. Reproducible quantitation of sheared and unsheared capsular antigen was demonstrated by rocket immunoelectrophoresis. As little as 2.5 ng of polyribophosphate pentose was identified and measured. Capsulated H. influenzae type b contained 78 ng of polyribophosphate pentose per mug of cell protein; class I contained 20 ng and class II contained 4 ng. The small amount of type b capsular antigen present in the class II variant may account for its lack of detection in this organism before now.", "contents": "Identification and quantitation of capsular antigen in capsulated and noncapsulated strains of Haemophilus influenzae type b by crossed-immunoelectrophoresis. Sonicated preparations of capsulated Haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class I variant) and the other noncapsulated (class II variant), and a noncapsulated strain of H. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed antiserum to capsulated H. influenzae type b. Twenty common antigens were present in all four cultures. Two type b-specific antigens were also identified in the three H. influenzae type b cultures when antiserum adsorbed with H. influenzae type d sonicates (AdR) was used. One of these, the type b capsular antigen, cross-reacted with an antigen of Bacillus pumilus. Further adsorption of AdR with the B. pumilus sonicate reduced, but did not eliminate, the antibodies to the type b capsular antigen, although all antibodies to B. pumilus were removed. Sonication sheared the type b capsular antigen, resulting in an increase in its electrophoretic mobility in agarose gel. The capsular antigen from all three H. influenzae type b cultures had the same electrophoretic characteristics. Reproducible quantitation of sheared and unsheared capsular antigen was demonstrated by rocket immunoelectrophoresis. As little as 2.5 ng of polyribophosphate pentose was identified and measured. Capsulated H. influenzae type b contained 78 ng of polyribophosphate pentose per mug of cell protein; class I contained 20 ng and class II contained 4 ng. The small amount of type b capsular antigen present in the class II variant may account for its lack of detection in this organism before now."}
{"id": "PMID:1085751", "title": "Lymphocyte populations in acute viral gastroenteritis.", "content": "Viral gastroenteritis was induced in 16 of 24 normal volunteers after oral administration of either the Norwalk or Hawaii agents. Clinical illness lasted for 24 to 48 h and resolved spontaneously. During acute illness, a transient lymphopenia was noted which involved all lymphocyte subpopulations (thymus-and bone marrow-derived, and null cells). No circulating lymphocytotoxins were detected, and the lymphocytes remaining in the circulation responded normally to mitogenic stimuli. The acute lymphopenia occurred at the time that mononuclear cell infiltration of the jejunal mucosa has been noted. These findings are consistent with the occurrence of a redistribution of circulating lymphocytes during acute illness, with accumulation of lymphocytes at the site of infection in the gut.", "contents": "Lymphocyte populations in acute viral gastroenteritis. Viral gastroenteritis was induced in 16 of 24 normal volunteers after oral administration of either the Norwalk or Hawaii agents. Clinical illness lasted for 24 to 48 h and resolved spontaneously. During acute illness, a transient lymphopenia was noted which involved all lymphocyte subpopulations (thymus-and bone marrow-derived, and null cells). No circulating lymphocytotoxins were detected, and the lymphocytes remaining in the circulation responded normally to mitogenic stimuli. The acute lymphopenia occurred at the time that mononuclear cell infiltration of the jejunal mucosa has been noted. These findings are consistent with the occurrence of a redistribution of circulating lymphocytes during acute illness, with accumulation of lymphocytes at the site of infection in the gut."}
{"id": "PMID:1085752", "title": "Immunodepression by Rowson-Parr virus in mice; lymphocyte markers and capping response of spleen and lymph node cells after infection.", "content": "Infection with Rowson-Parr virus (RPV) induced a rapid reduction in the number of immunoglobulin-positive and theta antigen-positive cells detectable by immunofluorescence in the spleens of susceptible BALB/c mice. The changes produced by RPV infection in the lymph nodes were different, since the number of immunoglobulin-positive cells was increased and the proportion of theta-positive cells remained unchanged. However, the ability of immunoglobulin-bearing cells to redistribute their receptors into caps was reduced in both types of lymphoid tissue. A similar pattern of changes was produced by infection with Friend leukemia complex, from which RPV was originally obtained. These effects of RPV and Friend leukemia complex may contribute to the immunodepressed state of infected mice.", "contents": "Immunodepression by Rowson-Parr virus in mice; lymphocyte markers and capping response of spleen and lymph node cells after infection. Infection with Rowson-Parr virus (RPV) induced a rapid reduction in the number of immunoglobulin-positive and theta antigen-positive cells detectable by immunofluorescence in the spleens of susceptible BALB/c mice. The changes produced by RPV infection in the lymph nodes were different, since the number of immunoglobulin-positive cells was increased and the proportion of theta-positive cells remained unchanged. However, the ability of immunoglobulin-bearing cells to redistribute their receptors into caps was reduced in both types of lymphoid tissue. A similar pattern of changes was produced by infection with Friend leukemia complex, from which RPV was originally obtained. These effects of RPV and Friend leukemia complex may contribute to the immunodepressed state of infected mice."}
{"id": "PMID:1085753", "title": "Platelet aggregation by hepatitis B surface antigen-antibody complexes.", "content": "Platelet aggregation indicating antigen-antibody complex formation was observed when hepatitis B surface (HBs) antigen and antibody were mixed. Platelet aggregation titers were determined for serum specimens found positive by radioimmunoassay for either HBs antigen or HBs antibody. From these determinations, incidence of HBs antigen-antibody complexes was found to be higher in HBs antigen seraas than in HBs antibody sera. There was an inverse correlation between platelet aggregation titers and radioimmunoassay values that was statistically significant for HBs antigen sera but not for HBs antibody sera. The incidence of anti-complementary activity was twice as high for platelet aggregation-positive HBs antigen and antibody sera as for platelet aggregation-negative sera. HBs antigen sera that were positive by platelet aggregation exhibited nearly three times the incidence of anti-complementary activity as did HBs antibody sera. However, the low incidence of anti-complementary activity was distributed about equally between HBs antigen and antibody sera that were negative by platelet aggregation. Additional HBs antigen preincubated with HBs antigen-positive sera effectively inhibited platelet aggregation, whereas additional HBs antibody was somewhat less effective. On the other hand, preincubation of HBs antigen sera with anti-IgG serum effectively enhanced platelet aggregation, whereas preincubation of HBs antigen sera with HBs antibody did not.", "contents": "Platelet aggregation by hepatitis B surface antigen-antibody complexes. Platelet aggregation indicating antigen-antibody complex formation was observed when hepatitis B surface (HBs) antigen and antibody were mixed. Platelet aggregation titers were determined for serum specimens found positive by radioimmunoassay for either HBs antigen or HBs antibody. From these determinations, incidence of HBs antigen-antibody complexes was found to be higher in HBs antigen seraas than in HBs antibody sera. There was an inverse correlation between platelet aggregation titers and radioimmunoassay values that was statistically significant for HBs antigen sera but not for HBs antibody sera. The incidence of anti-complementary activity was twice as high for platelet aggregation-positive HBs antigen and antibody sera as for platelet aggregation-negative sera. HBs antigen sera that were positive by platelet aggregation exhibited nearly three times the incidence of anti-complementary activity as did HBs antibody sera. However, the low incidence of anti-complementary activity was distributed about equally between HBs antigen and antibody sera that were negative by platelet aggregation. Additional HBs antigen preincubated with HBs antigen-positive sera effectively inhibited platelet aggregation, whereas additional HBs antibody was somewhat less effective. On the other hand, preincubation of HBs antigen sera with anti-IgG serum effectively enhanced platelet aggregation, whereas preincubation of HBs antigen sera with HBs antibody did not."}
{"id": "PMID:1085754", "title": "Morphology and time course of experimental listeriosis in nude mice.", "content": "Experimental listeriosis in phenotypically normal (nu/+) euthymic NMRI mice has a characteristic morphology and short-term course. In contrast, listeric infection in congenitally dysthymic nude (nu/nu)  mice does not proceed in clear-cut phases, develops more slowly, displays a chronic tendency from the beginning, and shows a considerably different morphology. The inability of nude mice to effectively control and terminate infection by Listeria monocytogenes obviously results from the lack of T lymphocytes.", "contents": "Morphology and time course of experimental listeriosis in nude mice. Experimental listeriosis in phenotypically normal (nu/+) euthymic NMRI mice has a characteristic morphology and short-term course. In contrast, listeric infection in congenitally dysthymic nude (nu/nu)  mice does not proceed in clear-cut phases, develops more slowly, displays a chronic tendency from the beginning, and shows a considerably different morphology. The inability of nude mice to effectively control and terminate infection by Listeria monocytogenes obviously results from the lack of T lymphocytes."}
{"id": "PMID:1085755", "title": "Transactions of an international conference. Worldwide concern: better dental care for more people. An international collaborative study. How the study was designed.", "content": "The study originated from the common need felt by the United States Public Health Service and the World Health Organization for objective information about various national dental care delivery systems and their effectiveness in the societies in which they were used. The relationships between consumers, providers and administrators within each system were emphasized rather than the comparison between systems since each operated within a specific biological and ecological environment. Six systems all of which had been in existence for at least twenty years were originally selected for study to encompass those dependent on a majority of government or private enterprise, use or non-use of auxiliaries, differeing systems of financing and of definition of target groups for receipt of services. The countries originally taking part were Australia, the Federal Republic of Germany, Japan, New Zealand, Norway and Bulgaria, though Bulgaria withdrew early in 1975.", "contents": "Transactions of an international conference. Worldwide concern: better dental care for more people. An international collaborative study. How the study was designed. The study originated from the common need felt by the United States Public Health Service and the World Health Organization for objective information about various national dental care delivery systems and their effectiveness in the societies in which they were used. The relationships between consumers, providers and administrators within each system were emphasized rather than the comparison between systems since each operated within a specific biological and ecological environment. Six systems all of which had been in existence for at least twenty years were originally selected for study to encompass those dependent on a majority of government or private enterprise, use or non-use of auxiliaries, differeing systems of financing and of definition of target groups for receipt of services. The countries originally taking part were Australia, the Federal Republic of Germany, Japan, New Zealand, Norway and Bulgaria, though Bulgaria withdrew early in 1975."}
{"id": "PMID:1085756", "title": "Preparation of various fractions from Mycobacterium smegmatis, their arthritogenicity and their preventive effect on adjuvant disease.", "content": "Cell walls of Mycobacterium smegmatis were able to produce much more severe arthritis in rats than the delipidated cells, whereas cell envelope and cell membrane fractions were unable to produce the disease. The lysozyme-solubilized product was able to produce mild disease with only 30% of incidence with an optimum dose, whereas the higher and the lower doses did not produce the disease. The rats immunized with cell envelope, cell membrane fraction and nonarthritogenic doses of lysozyme-solubilized product were protected against the subsequent homologous and heterologous challenge of delipidated cells. It was discussed that this preventative effect can be the result of antigenic competition between the arthritogenic and nonarthritogenic components of M. smegmatis. On the other hand, all the fractions separated here were able to serve as an immunoadjuvant in terms of inducing delayed hypersensitivity to ovalbumin in guinea pigs.", "contents": "Preparation of various fractions from Mycobacterium smegmatis, their arthritogenicity and their preventive effect on adjuvant disease. Cell walls of Mycobacterium smegmatis were able to produce much more severe arthritis in rats than the delipidated cells, whereas cell envelope and cell membrane fractions were unable to produce the disease. The lysozyme-solubilized product was able to produce mild disease with only 30% of incidence with an optimum dose, whereas the higher and the lower doses did not produce the disease. The rats immunized with cell envelope, cell membrane fraction and nonarthritogenic doses of lysozyme-solubilized product were protected against the subsequent homologous and heterologous challenge of delipidated cells. It was discussed that this preventative effect can be the result of antigenic competition between the arthritogenic and nonarthritogenic components of M. smegmatis. On the other hand, all the fractions separated here were able to serve as an immunoadjuvant in terms of inducing delayed hypersensitivity to ovalbumin in guinea pigs."}
{"id": "PMID:1085757", "title": "Arthritogenicity of Mycobacterium smegmatis subfractions, related to different oil vehicle and different composition.", "content": "Arthrigenicity of Mycobacterium smegmatis subfractions appeared to be remarkably potentiated in oil vehicles such as squalane or mineral oil, while water-in-oil emulsions containing Arlacel A appeared to decrease or suppress their arthritogenicity. It seems that Arlacel A can exert a suppressive effect on the arthritogenicity of the subfractions. Poly I:C and acetylated wax D potentiated the arthritogenicity of lysozyme-solubilized product, while cord factor was unable to do so. When given together with either cell membrane fraction or cell envelope, the lysozyme-solubilized product produced much more severe disease than that of lysozyme-solubilized product alone. Cell walls lost much of their arthritogenicity when mixed with lysozyme-solubilized product.", "contents": "Arthritogenicity of Mycobacterium smegmatis subfractions, related to different oil vehicle and different composition. Arthrigenicity of Mycobacterium smegmatis subfractions appeared to be remarkably potentiated in oil vehicles such as squalane or mineral oil, while water-in-oil emulsions containing Arlacel A appeared to decrease or suppress their arthritogenicity. It seems that Arlacel A can exert a suppressive effect on the arthritogenicity of the subfractions. Poly I:C and acetylated wax D potentiated the arthritogenicity of lysozyme-solubilized product, while cord factor was unable to do so. When given together with either cell membrane fraction or cell envelope, the lysozyme-solubilized product produced much more severe disease than that of lysozyme-solubilized product alone. Cell walls lost much of their arthritogenicity when mixed with lysozyme-solubilized product."}
{"id": "PMID:1085758", "title": "Dose-related effect of hydrocortisone treatment on the electrokinetic properties and mitogen responsiveness of mouse thymocytes.", "content": "The electrophoretic mobility (EPM) and the ability to synthesize DNA in vitro either spontaneously or in response to concanavalin A (Con A) and phytohemagglutinin (PHA) were investigated on thymocytes from adult female Swiss/B mice which had received intraperitoneally, 2 days previously, various doses (10-750 mg/kg body weight) of hydrocortisone acetate (OHC). A first group of thymocytes, highly sensitive to OHC, rapidly decreased in proportion following administration of increasing doses of OHC (10-125 mg/kg body weight) and included the totality of low-EPM thymocytes (EPM less than 1.0 mum sec-1 V-1 cm) together with about 70% of the high-EPM thymocytes encountered in the normal thymus. The second group of thymocytes (4% of the initial cellularity), which resisted to OHC at 125 mg/kg body weight and was only reduced by half with doses of OHC 6-fold larger, was composed essentially of high-EPM cells. The mean EPM of both groups tended to increase with increasing doses of OHC which suggests that among these two groups thymocytes are all the more OHC-sensitive as they possess a low surface charge. Spontaneous DNA synthesis, measurable on the first 24 h of in vitro cultivation, markedly dropped after treatment with OHC at doses higher than or equal to 62 mg/kg body weight. This loss of early spontaneous proliferation coincided with the disappearance of medium-EPM thymocytes. By contrast, proliferative response of thymocytes to mitogens was enhanced by OHC pretreatment of donors and roughly paralleled the enrichment in high-EPM cells. However, administration of the largest amounts of OHC diminished this mitogen responsiveness, although PHA reactivity was less affected than Con A reactivity.", "contents": "Dose-related effect of hydrocortisone treatment on the electrokinetic properties and mitogen responsiveness of mouse thymocytes. The electrophoretic mobility (EPM) and the ability to synthesize DNA in vitro either spontaneously or in response to concanavalin A (Con A) and phytohemagglutinin (PHA) were investigated on thymocytes from adult female Swiss/B mice which had received intraperitoneally, 2 days previously, various doses (10-750 mg/kg body weight) of hydrocortisone acetate (OHC). A first group of thymocytes, highly sensitive to OHC, rapidly decreased in proportion following administration of increasing doses of OHC (10-125 mg/kg body weight) and included the totality of low-EPM thymocytes (EPM less than 1.0 mum sec-1 V-1 cm) together with about 70% of the high-EPM thymocytes encountered in the normal thymus. The second group of thymocytes (4% of the initial cellularity), which resisted to OHC at 125 mg/kg body weight and was only reduced by half with doses of OHC 6-fold larger, was composed essentially of high-EPM cells. The mean EPM of both groups tended to increase with increasing doses of OHC which suggests that among these two groups thymocytes are all the more OHC-sensitive as they possess a low surface charge. Spontaneous DNA synthesis, measurable on the first 24 h of in vitro cultivation, markedly dropped after treatment with OHC at doses higher than or equal to 62 mg/kg body weight. This loss of early spontaneous proliferation coincided with the disappearance of medium-EPM thymocytes. By contrast, proliferative response of thymocytes to mitogens was enhanced by OHC pretreatment of donors and roughly paralleled the enrichment in high-EPM cells. However, administration of the largest amounts of OHC diminished this mitogen responsiveness, although PHA reactivity was less affected than Con A reactivity."}
{"id": "PMID:1085760", "title": "Radiosensitization of mammalian cells by diamide.", "content": "The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose-modifying factors of 0.81, 0.60 and 0.55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0.5 mM produced a dose-modifying factor of 0.34. When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat-blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes.", "contents": "Radiosensitization of mammalian cells by diamide. The effect of diamide on the radiosensitivity of T-cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose-modifying factors of 0.81, 0.60 and 0.55, respectively. Under anoxic conditions, exposure for 10 min before and during irradiation to 0.5 mM produced a dose-modifying factor of 0.34. When the cells in oxic conditions were exposed for just 20 min before irradiation, the sensitizing effect was smaller, but some sensitization effect was still apparent after a 120 min interval between diamide treatment and irradiation. Diamide also sensitized the cells after irradiation, but this effect was less than when it was present during irradiation. The presence of whole rat-blood in the incubation medium prevented sensitization. No sensitization could be detected in the whole animal. It is proposed that sensitization is due to lack of capacity for repair of radicals by hydrogen transfer and biochemical repair processes."}
{"id": "PMID:1085761", "title": "Control of DNA repair linked to neuroblastoma differentiation.", "content": "Mouse neuroblastoma cells, which can be induced to undergo reversible differentiation in culture, have been used as a model to investigate the effects of ultra-violet (U.V.) radiation on terminally-differentiated nerve cells. Differentiated neuroblastoma cells were found to be extremely sensitive to U.V.-radiation when compared with proliferating cells from the same clone. However, normal resistance was regained if the differentiated cells were allowed to proceed to the next G1 phase of the cell-cycle before irradiation. Neuroblastoma cells in the differentiated mode are capable of carrying out soem excision repair of DNA damage, but they appear to lack a repair mechanism present in proliferating cells.", "contents": "Control of DNA repair linked to neuroblastoma differentiation. Mouse neuroblastoma cells, which can be induced to undergo reversible differentiation in culture, have been used as a model to investigate the effects of ultra-violet (U.V.) radiation on terminally-differentiated nerve cells. Differentiated neuroblastoma cells were found to be extremely sensitive to U.V.-radiation when compared with proliferating cells from the same clone. However, normal resistance was regained if the differentiated cells were allowed to proceed to the next G1 phase of the cell-cycle before irradiation. Neuroblastoma cells in the differentiated mode are capable of carrying out soem excision repair of DNA damage, but they appear to lack a repair mechanism present in proliferating cells."}
{"id": "PMID:1085762", "title": "Studies on the radiosensitivity of the function of the Golgi complex.", "content": "The uptake of 3H-glucosamine into primary human-embryo fibroblasts and into the Golgi-rich fraction isolated from liver of mice labelled in vivo was studied, after various doses of X-radiation, by autoradiography and biochemical methods. A dose of 90 rad resulted in an increased precursor uptake in interphase cells at 24 hours and in mitotic cells at 48 hours after irradiation; 226 rad had virtually no effect on the grain counts of interphase cells, but reduced the labelling of mitotic forms. The characteristic intracellular localization of the grains were not influenced by these doses. Although no immediate radiation-induced reaction could be observed in liver cells either, significant stimulation of the 3H-glucosamine incorporation was measured in isolated Golgi-rich fractions 24 hours after whole-body irradiation with 90, 450, or 905 rad. This phenomenon is discussed as a part of the somatic regeneration of membrane structures.", "contents": "Studies on the radiosensitivity of the function of the Golgi complex. The uptake of 3H-glucosamine into primary human-embryo fibroblasts and into the Golgi-rich fraction isolated from liver of mice labelled in vivo was studied, after various doses of X-radiation, by autoradiography and biochemical methods. A dose of 90 rad resulted in an increased precursor uptake in interphase cells at 24 hours and in mitotic cells at 48 hours after irradiation; 226 rad had virtually no effect on the grain counts of interphase cells, but reduced the labelling of mitotic forms. The characteristic intracellular localization of the grains were not influenced by these doses. Although no immediate radiation-induced reaction could be observed in liver cells either, significant stimulation of the 3H-glucosamine incorporation was measured in isolated Golgi-rich fractions 24 hours after whole-body irradiation with 90, 450, or 905 rad. This phenomenon is discussed as a part of the somatic regeneration of membrane structures."}
{"id": "PMID:1085763", "title": "Effects of ionizing radiation and cysteamine (MEA) on activity of mouse spleen adenyl cyclase.", "content": "In mice X-irradiated with doses of 200 R and 400 R, there was a substantial increase in spleen adenyl cyclase activity; there was similar activation by MEA. In mice given MEA before irradiation, an additive effect of radiation and the radioprotective drug was observed. On the other hand, a dose of 800 R given either alone or after pre-treatment with MEA failed to elicit any change in cyclase activity. The results indicate the importance of the adenyl cyclase system in the response of cells to irradiation and action of MEA.", "contents": "Effects of ionizing radiation and cysteamine (MEA) on activity of mouse spleen adenyl cyclase. In mice X-irradiated with doses of 200 R and 400 R, there was a substantial increase in spleen adenyl cyclase activity; there was similar activation by MEA. In mice given MEA before irradiation, an additive effect of radiation and the radioprotective drug was observed. On the other hand, a dose of 800 R given either alone or after pre-treatment with MEA failed to elicit any change in cyclase activity. The results indicate the importance of the adenyl cyclase system in the response of cells to irradiation and action of MEA."}
{"id": "PMID:1085764", "title": "Change in the radiation responses of oogonia in the embryos and fry of the fish Oryzias latipes.", "content": "Embryos of the fish Oryzias latipes were irradiated with 1000 R of X-rays 1 day before hatching,and the post-irradiation change in the female germ-cell population was observed. Scarcely any reduction in the number of oogonia was observed, but their proliferation was inhibited. Repopulation occurred between 12 and 20 days after hatching. These responses were quite different from those of germ cells in the irradiated fry (Hamaguchi and Egami 1975). Embryos and/or fry were also exposed to 1000 R of X-rays 1 day before hatching and 0, 1, 2, and 3 days after hatching. A comparison of their responses suggested that the change in the radiation responses of oogonia is correlated with the initiation of meiosis.", "contents": "Change in the radiation responses of oogonia in the embryos and fry of the fish Oryzias latipes. Embryos of the fish Oryzias latipes were irradiated with 1000 R of X-rays 1 day before hatching,and the post-irradiation change in the female germ-cell population was observed. Scarcely any reduction in the number of oogonia was observed, but their proliferation was inhibited. Repopulation occurred between 12 and 20 days after hatching. These responses were quite different from those of germ cells in the irradiated fry (Hamaguchi and Egami 1975). Embryos and/or fry were also exposed to 1000 R of X-rays 1 day before hatching and 0, 1, 2, and 3 days after hatching. A comparison of their responses suggested that the change in the radiation responses of oogonia is correlated with the initiation of meiosis."}
{"id": "PMID:1085770", "title": "Histochemical characterization of cholinesterase activity in the frog brain with special reference to its localization on the wall of blood vessels.", "content": "The nature of the cholinesterase activity present on the wall of blood vessels in amphibian brain has been studied histochemically. It is concluded that the enzyme involved is acetylcholinesterase rather than butyrylcholinesterase, which more frequently occurs in the blood vessels of the brain of other vertebrates. The histochemical results are in agreement with most biochemical data concerning substrate specificity and inhibitor response for both acetylcholinesterase and butyrylcholinesterase. The two main hypotheses put forward by others in order to explain cholinesterase activity in brain vessels are discussed briefly.", "contents": "Histochemical characterization of cholinesterase activity in the frog brain with special reference to its localization on the wall of blood vessels. The nature of the cholinesterase activity present on the wall of blood vessels in amphibian brain has been studied histochemically. It is concluded that the enzyme involved is acetylcholinesterase rather than butyrylcholinesterase, which more frequently occurs in the blood vessels of the brain of other vertebrates. The histochemical results are in agreement with most biochemical data concerning substrate specificity and inhibitor response for both acetylcholinesterase and butyrylcholinesterase. The two main hypotheses put forward by others in order to explain cholinesterase activity in brain vessels are discussed briefly."}
{"id": "PMID:1085772", "title": "Mechanism of DNA degradation by the ATP-dependent DNase from Hemophilus influenzae Rd.", "content": "The rate of production of acid-soluble material during degradation of duplex DNA by Hemophilus influenzae ATP-dependent DNAse (Hind exonuclease V) has been shown to be directly dependent upon the Mg2+ concentration in the reaction mixture. At high concentrations of Mg2+ (5 to 20 mM), DNA degradation to acid-soluble products is rapid and the rate of ATP hydrolysis is slightly depressed. At low concentrations of Mg2+ (0.1 to 0.5 mM), the enzyme rapidly hydrolyzes ATP and converts up to 35% of linear duplex DNA to single-stranded material while degrading less than 0.2% of the DNA to acid-soluble products. We refer to this enzymatic production of single-stranded DNA as the \"melting\" activity. Under the conditions of our assay, the initial melting reaction is processive, lasting about 70s on phage T7 DNA. Using DNAs with several different lengths, we have established that the duration of the initial reaction is dependent upon DNA length, requiring approximately 1 s per 0.18 mum. The products of the initial reaction on phage T7 DNA are somewhat heterogeneous, consisting of short duplex fragments approximately 0.5 mum long, purely single-stranded products up to 7 mum long, and longer duplex fragments 3 to 11 mum in length, some of which have single-stranded tails. Nearly half of the single-stranded material remains linked to a duplex segment of DNA after the inital processive reaction. We propose that Hind exo V initiates attack at the DNA termini and then acts in a processive manner, migrating along the DNA molecule, converting some regions to single-stranded material by the combined action of the melting activity and limited phosphodiester cleavage, while leaving other regions double-stranded. At the completion of its processive movement through a single DNA molecule, it is released and then recycles onto either intact molecules or the partially degraded products, continuing in this manner until the DNA is finally reduced to oligonucleotides.", "contents": "Mechanism of DNA degradation by the ATP-dependent DNase from Hemophilus influenzae Rd. The rate of production of acid-soluble material during degradation of duplex DNA by Hemophilus influenzae ATP-dependent DNAse (Hind exonuclease V) has been shown to be directly dependent upon the Mg2+ concentration in the reaction mixture. At high concentrations of Mg2+ (5 to 20 mM), DNA degradation to acid-soluble products is rapid and the rate of ATP hydrolysis is slightly depressed. At low concentrations of Mg2+ (0.1 to 0.5 mM), the enzyme rapidly hydrolyzes ATP and converts up to 35% of linear duplex DNA to single-stranded material while degrading less than 0.2% of the DNA to acid-soluble products. We refer to this enzymatic production of single-stranded DNA as the \"melting\" activity. Under the conditions of our assay, the initial melting reaction is processive, lasting about 70s on phage T7 DNA. Using DNAs with several different lengths, we have established that the duration of the initial reaction is dependent upon DNA length, requiring approximately 1 s per 0.18 mum. The products of the initial reaction on phage T7 DNA are somewhat heterogeneous, consisting of short duplex fragments approximately 0.5 mum long, purely single-stranded products up to 7 mum long, and longer duplex fragments 3 to 11 mum in length, some of which have single-stranded tails. Nearly half of the single-stranded material remains linked to a duplex segment of DNA after the inital processive reaction. We propose that Hind exo V initiates attack at the DNA termini and then acts in a processive manner, migrating along the DNA molecule, converting some regions to single-stranded material by the combined action of the melting activity and limited phosphodiester cleavage, while leaving other regions double-stranded. At the completion of its processive movement through a single DNA molecule, it is released and then recycles onto either intact molecules or the partially degraded products, continuing in this manner until the DNA is finally reduced to oligonucleotides."}
{"id": "PMID:1085774", "title": "Unidirectional K+ fluxes in rat thymocytes stimulated by concanavalin A.", "content": "Unidirectional K+ fluxes were estimated in isolated rat thymocytes by 42K exchange kinetics. The cells were either preloaded with isotope and the release of it measured during incubation for one hour at 38 degrees C, or the cellular uptake of isotope during a similar incubation was measured. The influx rate of untreated thymocytes was: 2.3-10(-12) moles cm-2-s-1 and efflux rate: 1.8-10(-12) moles cm-2-s-1. When con A was added to the cells, influx was raised 74% and efflux 65%. Maximal effect was obtained when the concentration of con A was 15 mug/ml, but concentrations as low as 0.75 mug/ml were effective. Hydrocortisone resistant thymocytes responded at least was well as untreated cells to con A, which also raised RNA synthesis rate in the former cells 2.5 times. Using an extracellular marker, 51CrEDTA, intracellular concentrations of some ions was estimated in the thymocytes after one hour incubation: Na+: 30 mmoles/kg water, K+: 177 mmoles/kg water and Cl-:43 mmoles/kg water. Cellular water content: 69%. These values were not found significantly altered when con A was present. Since con A raised influx and efflux to the same extent and no net flux of K+ could be detected, it is proposed that both active and passive transport of K+ was increased by con A. The increased fluxes induced by con A, can apparently not be reversed by removal of con A from the incubation medium or by addition of the inhibiting hapten, alpha-methyl-D-mannoside.", "contents": "Unidirectional K+ fluxes in rat thymocytes stimulated by concanavalin A. Unidirectional K+ fluxes were estimated in isolated rat thymocytes by 42K exchange kinetics. The cells were either preloaded with isotope and the release of it measured during incubation for one hour at 38 degrees C, or the cellular uptake of isotope during a similar incubation was measured. The influx rate of untreated thymocytes was: 2.3-10(-12) moles cm-2-s-1 and efflux rate: 1.8-10(-12) moles cm-2-s-1. When con A was added to the cells, influx was raised 74% and efflux 65%. Maximal effect was obtained when the concentration of con A was 15 mug/ml, but concentrations as low as 0.75 mug/ml were effective. Hydrocortisone resistant thymocytes responded at least was well as untreated cells to con A, which also raised RNA synthesis rate in the former cells 2.5 times. Using an extracellular marker, 51CrEDTA, intracellular concentrations of some ions was estimated in the thymocytes after one hour incubation: Na+: 30 mmoles/kg water, K+: 177 mmoles/kg water and Cl-:43 mmoles/kg water. Cellular water content: 69%. These values were not found significantly altered when con A was present. Since con A raised influx and efflux to the same extent and no net flux of K+ could be detected, it is proposed that both active and passive transport of K+ was increased by con A. The increased fluxes induced by con A, can apparently not be reversed by removal of con A from the incubation medium or by addition of the inhibiting hapten, alpha-methyl-D-mannoside."}
{"id": "PMID:1085775", "title": "Genetic control of lipopolysaccharide induced generation of serum colony stimulating factor and proliferation of splenic granulocyte/macrophage precursor cells.", "content": "Administration of bacterial lipopolysaccharides (LPS) to mice causes a rise in tissue and serum colony stimulating factor (CSF) levels and in bone marrow and splenic colony forming cells (CFC). Two inbred strains of mice differing in their response to LPS were used to study the genetic control of LPS induced granulopoietic responses: a high responder strain (C3H/eB) which reacts to LPS by an elevation in serum CSF and by an increase in splenic CFC levels, and a low responder strain (C3H/HeJ) which fails to show these responses. The ability to generate serum CSF after administration of LPS is controlled by a single autosomal dominant gene, while the splenic CFC response to LPS follows the characteristic patterns of a polygenic inheritance control. The associated relationships of CSF and CFC responsiveness have been investigated in backcross (F1 X C3H/Hej) and F2 mice. Most mice which generated high levels of CSF showed a high or intermediate CFC response and most mice which did not generate any detectable levels of serum CSF showed a low splenic CFC response. The results suggest that CSF may play a physiologic role in vivo as a granulopoietin. In addition it was shown that the genetic control mechanisms governing the CSF/CFC responses are determined by the lipid A-KDO portion of the LPS molecule, suggesting that lipid A is the active part of the LPS molecule in stimulating granulopoiesis.", "contents": "Genetic control of lipopolysaccharide induced generation of serum colony stimulating factor and proliferation of splenic granulocyte/macrophage precursor cells. Administration of bacterial lipopolysaccharides (LPS) to mice causes a rise in tissue and serum colony stimulating factor (CSF) levels and in bone marrow and splenic colony forming cells (CFC). Two inbred strains of mice differing in their response to LPS were used to study the genetic control of LPS induced granulopoietic responses: a high responder strain (C3H/eB) which reacts to LPS by an elevation in serum CSF and by an increase in splenic CFC levels, and a low responder strain (C3H/HeJ) which fails to show these responses. The ability to generate serum CSF after administration of LPS is controlled by a single autosomal dominant gene, while the splenic CFC response to LPS follows the characteristic patterns of a polygenic inheritance control. The associated relationships of CSF and CFC responsiveness have been investigated in backcross (F1 X C3H/Hej) and F2 mice. Most mice which generated high levels of CSF showed a high or intermediate CFC response and most mice which did not generate any detectable levels of serum CSF showed a low splenic CFC response. The results suggest that CSF may play a physiologic role in vivo as a granulopoietin. In addition it was shown that the genetic control mechanisms governing the CSF/CFC responses are determined by the lipid A-KDO portion of the LPS molecule, suggesting that lipid A is the active part of the LPS molecule in stimulating granulopoiesis."}
{"id": "PMID:1085776", "title": "Hemophilus influenzae, type b, antibody frequencies determined with bactericidal and radioimmunoassay tests.", "content": "Bactericidal and radioimmunoassay (RIA) antibodies to Hemophilus influenzae, type b, were measured in the sera of 85 children aged 18 through 71 months. Bactericidal antibodies were present in only 21% of those over 60 months; RIA levels rose linearly with increasing age (P less than 0.005). Bactericidal antibodies were present in nine of 29 sera with RIA levels greater than or equal to 0.7 mug/ml and in only four of 56 sera with RIA levels less than 0.7 mug/ml (P less than 0.01). The discrepancies between the two tests probably reflect their measurement of different antibodies. Results of the RIA fit the clinical observation that H. influenzae meningitis becomes less frequent with age.", "contents": "Hemophilus influenzae, type b, antibody frequencies determined with bactericidal and radioimmunoassay tests. Bactericidal and radioimmunoassay (RIA) antibodies to Hemophilus influenzae, type b, were measured in the sera of 85 children aged 18 through 71 months. Bactericidal antibodies were present in only 21% of those over 60 months; RIA levels rose linearly with increasing age (P less than 0.005). Bactericidal antibodies were present in nine of 29 sera with RIA levels greater than or equal to 0.7 mug/ml and in only four of 56 sera with RIA levels less than 0.7 mug/ml (P less than 0.01). The discrepancies between the two tests probably reflect their measurement of different antibodies. Results of the RIA fit the clinical observation that H. influenzae meningitis becomes less frequent with age."}
{"id": "PMID:1085777", "title": "Comparison of isolation of Haemophilus vaginalis (Corynebacterium vaginale) from peptone-starch-dextrose agar and Columbia colistin-nalidoxic acid agar.", "content": "A total of 447 cervical or vaginal specimens were inoculated in parallel onto peptone-starch-dextrose (PSD) and Columbia colistin (10 mg/ml)-nalidixic acid (15 mug/ml) (CNA) agar and were incubated for 48 h at 35 degrees C in an atmosphere with 2 to 10% CO2. One hundred (22.4%) of the cultures were positive for Haemophilus vaginalis. Forty-eight of the isolates were recovered from both PSD and Columbia CNA agar, five from PSD only, and 47 from Columbia CNA agar only (P less than 0.001). On Columbia CNA agar, 76 of the isolates were detected after 24 h of incubation, and the remainder were detected within 4 days of incubation.", "contents": "Comparison of isolation of Haemophilus vaginalis (Corynebacterium vaginale) from peptone-starch-dextrose agar and Columbia colistin-nalidoxic acid agar. A total of 447 cervical or vaginal specimens were inoculated in parallel onto peptone-starch-dextrose (PSD) and Columbia colistin (10 mg/ml)-nalidixic acid (15 mug/ml) (CNA) agar and were incubated for 48 h at 35 degrees C in an atmosphere with 2 to 10% CO2. One hundred (22.4%) of the cultures were positive for Haemophilus vaginalis. Forty-eight of the isolates were recovered from both PSD and Columbia CNA agar, five from PSD only, and 47 from Columbia CNA agar only (P less than 0.001). On Columbia CNA agar, 76 of the isolates were detected after 24 h of incubation, and the remainder were detected within 4 days of incubation."}
{"id": "PMID:1085778", "title": "The paradox of Hemophilus infuenzae type B bacteremia in the presence of serum bactericidal activity.", "content": "We investigated the role of serum bactericidal activity in Hemophiplus influenzae type b infections in infants with meningitis and in a rat model. In infected infants, 13/22 admission sera had bactericidal activity against the infecting strain, and bacteremia was as frequent in those with bactericidal activity (54%) as those without (56%). The coexistence of bactericidal activity and bacteremia was reproduced and studied in experimentally infected weanling rats. Serum from such rats kills in vitro 95% of conventionally broth-grown bacteria within 10 min, but does not kill organisms obtained from the infected animals. Thus bactericidal activity as conventionally determined for H. influenzae b may have no relevance in vivo, Incubation of broth-grown bacteria in normal rat serum for 30 min at 37 degrees C produces a resistance like that of in vivo organisms. This phenotypic conversion depends on factors that are of molecular weight less than 1,000, stable to 100 degrees C, but destroyed by ashing. When injected intravenously into nonimmune animals, broth-grown bacteria are quickly cleared, while serum-preincubated bacteria are not. The latter, however, are cleared when injected into bacteremic rats (half-life 30 min). Bacteremia in the rats may persist despite this capacity for clearance because bacteria are entering the blood from extravascular fluids, which contain greater than 90% of the total bacterial burden.", "contents": "The paradox of Hemophilus infuenzae type B bacteremia in the presence of serum bactericidal activity. We investigated the role of serum bactericidal activity in Hemophiplus influenzae type b infections in infants with meningitis and in a rat model. In infected infants, 13/22 admission sera had bactericidal activity against the infecting strain, and bacteremia was as frequent in those with bactericidal activity (54%) as those without (56%). The coexistence of bactericidal activity and bacteremia was reproduced and studied in experimentally infected weanling rats. Serum from such rats kills in vitro 95% of conventionally broth-grown bacteria within 10 min, but does not kill organisms obtained from the infected animals. Thus bactericidal activity as conventionally determined for H. influenzae b may have no relevance in vivo, Incubation of broth-grown bacteria in normal rat serum for 30 min at 37 degrees C produces a resistance like that of in vivo organisms. This phenotypic conversion depends on factors that are of molecular weight less than 1,000, stable to 100 degrees C, but destroyed by ashing. When injected intravenously into nonimmune animals, broth-grown bacteria are quickly cleared, while serum-preincubated bacteria are not. The latter, however, are cleared when injected into bacteremic rats (half-life 30 min). Bacteremia in the rats may persist despite this capacity for clearance because bacteria are entering the blood from extravascular fluids, which contain greater than 90% of the total bacterial burden."}
{"id": "PMID:1085773", "title": "Experimental evaluation and clinical application of intraoperative counterpulsation without balloon pumping.", "content": "Cardiac assistance in the form of counterpulsation (CP), created by intraaortic balloon pumping (IABP), has recently been utilized to wean patients from cardiopulmonary bypass (CPB) after conventional modalities have failed. The use of IABP, however, is often delayed because it is not part of the standard CPB setup and requires an additional cutdown, an arterial graft, and the retrograde insertion of a foreign body into the arterial tree. To eliminate the disadvantages of IABP, an alternate technique for CP has been developed. A pneumatially actuated external blood reservoir connected via a \"Y\" connector to the standard arterial line of the CPB circuit can provide pulsatile flow, and when synchronized with the EKG, CP with or without CPB. Experimental evaluation with dogs comparing this technique with IABP has shown that the former can create comparable CP pressure curves to the latter. The technique was applied to 11 patients undergoing either aorto-coronary bypass surgery and/or valvular surgery. Two of the 11 patients could come off CPB only with the assistance of CP. Coronary bypass graft flow, when measured, increased by 25% due to CP in two out of seven patients. The arterial CP technique has proven to be easily adapted to clinical settings and provides immediate and effective CP when required.", "contents": "Experimental evaluation and clinical application of intraoperative counterpulsation without balloon pumping. Cardiac assistance in the form of counterpulsation (CP), created by intraaortic balloon pumping (IABP), has recently been utilized to wean patients from cardiopulmonary bypass (CPB) after conventional modalities have failed. The use of IABP, however, is often delayed because it is not part of the standard CPB setup and requires an additional cutdown, an arterial graft, and the retrograde insertion of a foreign body into the arterial tree. To eliminate the disadvantages of IABP, an alternate technique for CP has been developed. A pneumatially actuated external blood reservoir connected via a \"Y\" connector to the standard arterial line of the CPB circuit can provide pulsatile flow, and when synchronized with the EKG, CP with or without CPB. Experimental evaluation with dogs comparing this technique with IABP has shown that the former can create comparable CP pressure curves to the latter. The technique was applied to 11 patients undergoing either aorto-coronary bypass surgery and/or valvular surgery. Two of the 11 patients could come off CPB only with the assistance of CP. Coronary bypass graft flow, when measured, increased by 25% due to CP in two out of seven patients. The arterial CP technique has proven to be easily adapted to clinical settings and provides immediate and effective CP when required."}
{"id": "PMID:1085780", "title": "Ontogenetic shift of spectral phototactic preferences in anuran tadpoles.", "content": "Tadpoles of three species of anurans initially had a midspectrum (\"green\") preference in laboratory phototactic tests, which was shown experimentally to involve a form of true color vision in one species and probably in the other two as well. During development, the preference shifted to shorter wave-lengths (higher frequencies) until a short-wavelength (\"blue\") preference predominated in the pre- and postmetamorphic stages and in the adults of six species tested; color vision was involved in all of these stages. The green preference of young tadpoles is ecologically adaptive, in that it directs larvae to green plants that provide food or shelter. Tadpoles observed in a pond congregated heavily in vegetated areas rather than in open water. Spectroradiometric field measurements showed that pond illumination in vegetated areas had a more highly saturated yellow-green spectral dominance compared with a desaturated white illumination in open water. During all ontogenetic stages and as adults, the animals had a preference for high illuminance of white light, which correlates with the high illumination of their habitat. Microspectrophotometric data from Liebman and Entine suggested that the green rods are active receptors in tadpoles, making unlikely Muntz's hypothesis that the ontogenetic shift in spectral preferences is due to premetamorphic maturation of these receptors. However, the visual pigments of all five types of photoreceptors shifted from vitamin A2- to vitamin A1-based chromophores during ontogeny, and the resulting shift in spectral response of the receptors might be related to the spectral shift in phototactic preferences.", "contents": "Ontogenetic shift of spectral phototactic preferences in anuran tadpoles. Tadpoles of three species of anurans initially had a midspectrum (\"green\") preference in laboratory phototactic tests, which was shown experimentally to involve a form of true color vision in one species and probably in the other two as well. During development, the preference shifted to shorter wave-lengths (higher frequencies) until a short-wavelength (\"blue\") preference predominated in the pre- and postmetamorphic stages and in the adults of six species tested; color vision was involved in all of these stages. The green preference of young tadpoles is ecologically adaptive, in that it directs larvae to green plants that provide food or shelter. Tadpoles observed in a pond congregated heavily in vegetated areas rather than in open water. Spectroradiometric field measurements showed that pond illumination in vegetated areas had a more highly saturated yellow-green spectral dominance compared with a desaturated white illumination in open water. During all ontogenetic stages and as adults, the animals had a preference for high illuminance of white light, which correlates with the high illumination of their habitat. Microspectrophotometric data from Liebman and Entine suggested that the green rods are active receptors in tadpoles, making unlikely Muntz's hypothesis that the ontogenetic shift in spectral preferences is due to premetamorphic maturation of these receptors. However, the visual pigments of all five types of photoreceptors shifted from vitamin A2- to vitamin A1-based chromophores during ontogeny, and the resulting shift in spectral response of the receptors might be related to the spectral shift in phototactic preferences."}
{"id": "PMID:1085781", "title": "Immunoglobulin E synthesis in parasite infection.", "content": "The infection of rats with Nippostrongylus brasiliensis (Nb) larvae enhanced IgE synthesis prior to the formation of IgE antibody specific for parasite antigens. As early as the eighth to tenth day of infection, IgE-bearing lymphocytes appeared in lymph nodes and spleen. At this time, even bone marrow contained IgE-bearing blast cells. The IgE-forming plasma cells were detected in the lymph nodes and spleen at the tenth day and their number reached maximum at the fourteenth day. The proliferation of IgE-bearing lymphocytes in the lymphoid tissues was observed in neonatally thymectomized animals, indicating that T cells are not essential for the development of IgE-B cells in the infected animals. It appears, however, that T cells are involved in the differentiation of IgE-B cells to IgE-forming plasma cells. Nonspecific proliferation of IgE-B cells and their differentiation to IgE-forming plasma cells may explain potentiation of IgE antibody formation against unrelated antigens after infection with N. brasiliensis (Nb). It was also found that T cells specific for parasite antigens were primed by infection with Nb. Evidence was obtained tht Nb-specific T cells raised by the infection collaborated with hapten-specific IgE-B cells and IgG-B cells for the secondary antihapten antibody responses. By contrast, T cells primed by immunization with parasite antigen included in alum exerted the helper function for IgG antibody response but failed to collaborate with IgE-B cells. Dissociation between the helper function for IgE and IgG antibody response indicated that parasite infection generated a favorable condition for priming T cells for the IgE antibody response.", "contents": "Immunoglobulin E synthesis in parasite infection. The infection of rats with Nippostrongylus brasiliensis (Nb) larvae enhanced IgE synthesis prior to the formation of IgE antibody specific for parasite antigens. As early as the eighth to tenth day of infection, IgE-bearing lymphocytes appeared in lymph nodes and spleen. At this time, even bone marrow contained IgE-bearing blast cells. The IgE-forming plasma cells were detected in the lymph nodes and spleen at the tenth day and their number reached maximum at the fourteenth day. The proliferation of IgE-bearing lymphocytes in the lymphoid tissues was observed in neonatally thymectomized animals, indicating that T cells are not essential for the development of IgE-B cells in the infected animals. It appears, however, that T cells are involved in the differentiation of IgE-B cells to IgE-forming plasma cells. Nonspecific proliferation of IgE-B cells and their differentiation to IgE-forming plasma cells may explain potentiation of IgE antibody formation against unrelated antigens after infection with N. brasiliensis (Nb). It was also found that T cells specific for parasite antigens were primed by infection with Nb. Evidence was obtained tht Nb-specific T cells raised by the infection collaborated with hapten-specific IgE-B cells and IgG-B cells for the secondary antihapten antibody responses. By contrast, T cells primed by immunization with parasite antigen included in alum exerted the helper function for IgG antibody response but failed to collaborate with IgE-B cells. Dissociation between the helper function for IgE and IgG antibody response indicated that parasite infection generated a favorable condition for priming T cells for the IgE antibody response."}
{"id": "PMID:1085782", "title": "Comparative anatomy of cerebellar catecholamine innervation from teleosts to mammals.", "content": "The cerebellar innervation of the catecholamine (CA) neurons was experimentally investigated with histofluorescence method in the brains of the vertebrates from teleosts to mammals. In the teleosts the source for the cerebellar CA are composed of three CA neuron groups. Hypothalamic CA neuron group contributes mainly to innervation in the valvula cerebelli whose development is dependent on the high degree of the development of the lateral-line organ. CA neurons in the medulla oblongata might innervate the vestibulo-lateral lobe and corpus cerebelli. These two CA neuron groups appear to constitute the main source for the cerebellar CA, though it is certain that rhombencephalic CA neurons also send their axons to innervate the cerebellum. In the tailless amphibian which is devoid of the lateral-line nerves, cerebellar CA especially in the corpus cerebelli is supplied mainly by the rhombencephalic CA neurons which still remain in less developed stage in phylogenetic scale. In the bird, ascending CA neuron system is maintained by long axons and the anlage of locus coeruleus (LC) appear first in this class. A large number of CA nerve terminals in the cerebellum of the bird are supplied by both CA neurons of the dorsal part of the tegmentum and CA neurons disseminated in the tegmental reticular formation. In the mammals showing the highest development of the cerebellar and cerebral cortices, LC is well developed and cerebellar CA is supplied mainly by LC, though cerebellum is innervated by other CA neurons besides LC. Possible significance of the CA neurons on the development of the cerebellum in phylogeny was discussed.", "contents": "Comparative anatomy of cerebellar catecholamine innervation from teleosts to mammals. The cerebellar innervation of the catecholamine (CA) neurons was experimentally investigated with histofluorescence method in the brains of the vertebrates from teleosts to mammals. In the teleosts the source for the cerebellar CA are composed of three CA neuron groups. Hypothalamic CA neuron group contributes mainly to innervation in the valvula cerebelli whose development is dependent on the high degree of the development of the lateral-line organ. CA neurons in the medulla oblongata might innervate the vestibulo-lateral lobe and corpus cerebelli. These two CA neuron groups appear to constitute the main source for the cerebellar CA, though it is certain that rhombencephalic CA neurons also send their axons to innervate the cerebellum. In the tailless amphibian which is devoid of the lateral-line nerves, cerebellar CA especially in the corpus cerebelli is supplied mainly by the rhombencephalic CA neurons which still remain in less developed stage in phylogenetic scale. In the bird, ascending CA neuron system is maintained by long axons and the anlage of locus coeruleus (LC) appear first in this class. A large number of CA nerve terminals in the cerebellum of the bird are supplied by both CA neurons of the dorsal part of the tegmentum and CA neurons disseminated in the tegmental reticular formation. In the mammals showing the highest development of the cerebellar and cerebral cortices, LC is well developed and cerebellar CA is supplied mainly by LC, though cerebellum is innervated by other CA neurons besides LC. Possible significance of the CA neurons on the development of the cerebellum in phylogeny was discussed."}
{"id": "PMID:1085783", "title": "Comparative anatomy of the locus coeruleus. II. Organization and projection of the catecholamine containing neurons in the upper rhombencephalon of the frog, Rana catesbiana.", "content": "The organization and projection of the catecholamine (CA) containing neurons in the upper rhombencephalon were experimentally investigated with histofluorescence method in the brain of the bullfrog, Rana catesbiana. A small number of catecholamine containing neurons are located in the upper rhombencephalon. These neurons do not send ascending fibers to contribute CA projection to the forebrain, but give rise to relatively short axons to innervate the cerebellum and neighbouring reticular formation. Judging from their anatomical aspects, it seems that the structure homologous to the mammalian locus coeruleus is not present in the amphibian brain.", "contents": "Comparative anatomy of the locus coeruleus. II. Organization and projection of the catecholamine containing neurons in the upper rhombencephalon of the frog, Rana catesbiana. The organization and projection of the catecholamine (CA) containing neurons in the upper rhombencephalon were experimentally investigated with histofluorescence method in the brain of the bullfrog, Rana catesbiana. A small number of catecholamine containing neurons are located in the upper rhombencephalon. These neurons do not send ascending fibers to contribute CA projection to the forebrain, but give rise to relatively short axons to innervate the cerebellum and neighbouring reticular formation. Judging from their anatomical aspects, it seems that the structure homologous to the mammalian locus coeruleus is not present in the amphibian brain."}
{"id": "PMID:1085784", "title": "Single step separation of human T and B cells using AET treated srbc rosettes.", "content": "A technique for the single step separation of human thymus derived (T) and Bursa equivalent (B) lymphocytes was developed. Density separation of B lymphocytes and T lymphocyte-sheep red blood cell (SRBC) rosettes on Ficoll-Hypaque was modified by pretreatment of the SRBC with 2 aminoethylisothiouronium bromide hydrobromide. This modification yielded significantly purer populations of T and B cells. Up to 15 X 10(7) were separated without increasing contamination allowing for the recovery of B lymphocytes as well as T lymphocytes in sufficient numbers to use in functional assays.", "contents": "Single step separation of human T and B cells using AET treated srbc rosettes. A technique for the single step separation of human thymus derived (T) and Bursa equivalent (B) lymphocytes was developed. Density separation of B lymphocytes and T lymphocyte-sheep red blood cell (SRBC) rosettes on Ficoll-Hypaque was modified by pretreatment of the SRBC with 2 aminoethylisothiouronium bromide hydrobromide. This modification yielded significantly purer populations of T and B cells. Up to 15 X 10(7) were separated without increasing contamination allowing for the recovery of B lymphocytes as well as T lymphocytes in sufficient numbers to use in functional assays."}
{"id": "PMID:1085785", "title": "A simple method for screening human T and B lymphocyte alloantibodies.", "content": "An sRBC-hemolysin monolayer was employed for separating human lymphocytes into two sub-populations, adherent cells (predominantly B cells) and non-adherent cells (predominantly T cells). The separated cells were then tested simultaneously against predispensed sera by complement-mediated cytolysis for the presence of alloantibodies to either T or B cells. A total of 372 sera were screened. Antibodies specific for B cell antigens were found in 19 sera and 9 sera were found to contain antibodies to T-specific antigens.", "contents": "A simple method for screening human T and B lymphocyte alloantibodies. An sRBC-hemolysin monolayer was employed for separating human lymphocytes into two sub-populations, adherent cells (predominantly B cells) and non-adherent cells (predominantly T cells). The separated cells were then tested simultaneously against predispensed sera by complement-mediated cytolysis for the presence of alloantibodies to either T or B cells. A total of 372 sera were screened. Antibodies specific for B cell antigens were found in 19 sera and 9 sera were found to contain antibodies to T-specific antigens."}
{"id": "PMID:1085786", "title": "The sealed capillary migration technique and thymocyte migration in vitro.", "content": "The in vitro migration of thymocytes in sealed capillary tubes was influenced by cell number, temperature, cell viability and oxygen supply. Migration was successively slower over a 24 h period. There was no major influence of gravity or the degree of initial packing of the cells by centrifugation. Migration was inhibited by cytochalasin B, although the cells escaped from this effect after 8 h. A transient stimulatory effect on migration was seen after addition of serum or supernatants from cultured thymocytes. There was no effect of isoproterenol, theophylline or carbacholine. A paradoxic effect was obtained with high doses of some metabolic inhibitors, which produced increased migration in spite of cell death. The technique may be used for studies on lymphocyte migration in vitro, but care must be taken to exclude a toxic influence of added substances, and expected effects should preferably be looked for early after onset of migration, since cell viability is gradually decreased.", "contents": "The sealed capillary migration technique and thymocyte migration in vitro. The in vitro migration of thymocytes in sealed capillary tubes was influenced by cell number, temperature, cell viability and oxygen supply. Migration was successively slower over a 24 h period. There was no major influence of gravity or the degree of initial packing of the cells by centrifugation. Migration was inhibited by cytochalasin B, although the cells escaped from this effect after 8 h. A transient stimulatory effect on migration was seen after addition of serum or supernatants from cultured thymocytes. There was no effect of isoproterenol, theophylline or carbacholine. A paradoxic effect was obtained with high doses of some metabolic inhibitors, which produced increased migration in spite of cell death. The technique may be used for studies on lymphocyte migration in vitro, but care must be taken to exclude a toxic influence of added substances, and expected effects should preferably be looked for early after onset of migration, since cell viability is gradually decreased."}
{"id": "PMID:1085788", "title": "Ototoxicity of tobramycin: a clinical overview.", "content": "In a survey of data on 3,506 patients treated with tobramycin, the 21 cases of drug-related ototoxicity are reported and reviewed. In seven patients the effects were auditory only, in nine vestibular only, and in five both auditory and vestibular. Effects subsided in 14 of the 18 patients who were available for monitoring after therapy. High-frequency audiometric losses persisted in three patients, and a hearing decrease persisted in one. In only one of the three patients did audiometric losses exceed 40 decibels. The patients with drug-related ototoxicity were compared to a group of 49 patients for whom both audiometric testing (before and after therapy) and clinical observation showed that no ototoxic reactions had occurred. Preexisting renal impairment, prior and/or concomitant therapy with other possibly ototoxic drugs, and therapy for 10 days or more with a dose of greater than 3 mg/kg per day were found to be associated with ototoxicity.", "contents": "Ototoxicity of tobramycin: a clinical overview. In a survey of data on 3,506 patients treated with tobramycin, the 21 cases of drug-related ototoxicity are reported and reviewed. In seven patients the effects were auditory only, in nine vestibular only, and in five both auditory and vestibular. Effects subsided in 14 of the 18 patients who were available for monitoring after therapy. High-frequency audiometric losses persisted in three patients, and a hearing decrease persisted in one. In only one of the three patients did audiometric losses exceed 40 decibels. The patients with drug-related ototoxicity were compared to a group of 49 patients for whom both audiometric testing (before and after therapy) and clinical observation showed that no ototoxic reactions had occurred. Preexisting renal impairment, prior and/or concomitant therapy with other possibly ototoxic drugs, and therapy for 10 days or more with a dose of greater than 3 mg/kg per day were found to be associated with ototoxicity."}
{"id": "PMID:1085809", "title": "Frog lysozyme III. The effects of metamorphic inhibition on Rana pipiens lysozyme ontogeny.", "content": "The inhibition of Rana pipiens metamorphosis by thiouracil altered the ontogeny of lysozyme. Certain isozymes of the enzyme remained absent. There was, nevertheless, an increase in tissue lysozyme concentration.", "contents": "Frog lysozyme III. The effects of metamorphic inhibition on Rana pipiens lysozyme ontogeny. The inhibition of Rana pipiens metamorphosis by thiouracil altered the ontogeny of lysozyme. Certain isozymes of the enzyme remained absent. There was, nevertheless, an increase in tissue lysozyme concentration."}
{"id": "PMID:1085812", "title": "[A case of Turner's syndrome in 45,X/46,XXp- mosaicism associated with colour-blindness (author's transl)].", "content": "We have reported the study of a young girl aged 19 suffering from a gonadal dysgenesis the chromosomal complement of which is 45,X/46,XXp-. The analysis of the transmission of Xg group was insufficient to demonstrate with certainty the origin of the pathological X. The tests indicating the ability to discriminate colours (Ishihara's test and anomaloscopy) showed a protanopia, probably of paternal origin. Hence, the Xp- probably comes from the mother.", "contents": "[A case of Turner's syndrome in 45,X/46,XXp- mosaicism associated with colour-blindness (author's transl)]. We have reported the study of a young girl aged 19 suffering from a gonadal dysgenesis the chromosomal complement of which is 45,X/46,XXp-. The analysis of the transmission of Xg group was insufficient to demonstrate with certainty the origin of the pathological X. The tests indicating the ability to discriminate colours (Ishihara's test and anomaloscopy) showed a protanopia, probably of paternal origin. Hence, the Xp- probably comes from the mother."}
{"id": "PMID:1085814", "title": "Habituation of a monosynaptic response in frog spinal cord: evidence for a presynaptic mechanism.", "content": "1. Using the isolated spinal cord of bullfrogs (Rana catesbeiana), intracellular correlates of habituation-like depression of the monosynaptic response elicited in motoneurons by lateral column (LC) stimulation were investigated. The following properties of the motoneuron were compared before and after response depression produced by stimulation of the LC at 0.5/s: resting membrane potential, membrane conductance, critical firing level, and rheobasic current. No alteration was found in any of these parameters. 2. To determine whether transmitter release mechanisms were changing over trials, the LC was stimulated with pairs of stimuli separated by 6 ms presented at 0.5/s. While the amplitude of the first EPSP declined (74% of initial value), the amplitude of the second EPSP increased (111% of initial value). Facilitation ratios thus increased. 3. The following conclusions can thus be drawn: 1) habituation involves a process intrinsic to the LC-motoneuron synapse; 2) habituation is not totally mediated by receptor desensitization; 3) habituation is not mediated by a mechanism extrinsic to the LC-motoneuron synapse that depolarizes terminal endings, e.g., presynaptic inhibition or accumulation of extracellular potassium; 4) habituation is not produced by transmitter depletion. Any of these possibilities has as a necessary consequence that facilitiation ratios remain unchanged. 4. Possible mechanisms that could mediate habituation are: 1) alterations in mobilization  and/or release of transmitter; 2) decreased probability of invasion of terminal branches of the presynaptic fiber by the action potential.", "contents": "Habituation of a monosynaptic response in frog spinal cord: evidence for a presynaptic mechanism. 1. Using the isolated spinal cord of bullfrogs (Rana catesbeiana), intracellular correlates of habituation-like depression of the monosynaptic response elicited in motoneurons by lateral column (LC) stimulation were investigated. The following properties of the motoneuron were compared before and after response depression produced by stimulation of the LC at 0.5/s: resting membrane potential, membrane conductance, critical firing level, and rheobasic current. No alteration was found in any of these parameters. 2. To determine whether transmitter release mechanisms were changing over trials, the LC was stimulated with pairs of stimuli separated by 6 ms presented at 0.5/s. While the amplitude of the first EPSP declined (74% of initial value), the amplitude of the second EPSP increased (111% of initial value). Facilitation ratios thus increased. 3. The following conclusions can thus be drawn: 1) habituation involves a process intrinsic to the LC-motoneuron synapse; 2) habituation is not totally mediated by receptor desensitization; 3) habituation is not mediated by a mechanism extrinsic to the LC-motoneuron synapse that depolarizes terminal endings, e.g., presynaptic inhibition or accumulation of extracellular potassium; 4) habituation is not produced by transmitter depletion. Any of these possibilities has as a necessary consequence that facilitiation ratios remain unchanged. 4. Possible mechanisms that could mediate habituation are: 1) alterations in mobilization  and/or release of transmitter; 2) decreased probability of invasion of terminal branches of the presynaptic fiber by the action potential."}
{"id": "PMID:1085815", "title": "Sound localization in anurans. I. Evidence of binaural interaction in dorsal medullary nucleus of bullfrogs (Rana catesbeiana).", "content": "1. The response patterns of single cells to monaural and binaural acoustic stimuli were studied in the dorsal medullary nucleus of the bullfrog (Rana catesbeiana). This nucleus represents the first ascending center in the anuran's central auditory nervous system. 2. Of the 142 cells isolated, 75 units responded only to monaural stimulation. Approximately 80% of these monaural cells could be excited by the ipsilateral ear, while the remaining 20% received their excitatory input from the contralateral ear. The other 67 units responded to binaural stimuli. Of these binaural cells, 14 could be excited by either contralateral or ipsilateral stimuli, and the threshold and best excitatory frequency were similar for each ear (EE). The other 53 binaural cells (EI) could be excited by stimulation of one ear and inhibited by stimulation of the other ear; for almost all of these cells the contralateral ear was excitatory and the ipsilateral ear was inhibitory. The best inhibitory frequency for one ear was approximately the same as the best excitatory frequency for the other ear, and the threshold for inhibition was near the threshold for excitation. 3. The tuning curves for all of the cells in the dorsal medullary nucleus were unimodal with \"Q\" values ranging from 0.4 to 4. The excitatory thresholds were widely scattered between 22 and 115 dB SPL. 4. The distribution of best excitatory frequencies for the monaural cells comprised three groups: 200-300, 500-800, and 900-1,600 Hz. The best excitatory frequencies of the binaural cells were scattered over this entire range, with a broad peak around 200-800 Hz. 5. Approximately 80% of the cells in the dorsal nucleus responded tonically throughout the duration of an excitatory tone burst. The remaining 20% of the cells responded phasically during the transient stages of a tone burst over a wide intensity range. 6. Response latencies were compared for the two types of monaural cells to tones at their best exciatatory frequencies at 10 dB above threshold. The latencies for the contralaterally excitable cells were just a few milliseconds longer than the latencies for the ipsilaterally excitable cells. For binaural cells the latency for contralateral stimulation was only 1-2 ms longer than for ipsilateral stimulation. It was concluded that the contralateral input to the dorsal medullary nucleus is not of efferent descending origin from higher auditory centers. 7. All of the binaural EI cells were sensitive to small interaural intensity differences and many were also sensitive to minute interaural time differences. These cells likely play a role in localization of sounds of significance to anurans.", "contents": "Sound localization in anurans. I. Evidence of binaural interaction in dorsal medullary nucleus of bullfrogs (Rana catesbeiana). 1. The response patterns of single cells to monaural and binaural acoustic stimuli were studied in the dorsal medullary nucleus of the bullfrog (Rana catesbeiana). This nucleus represents the first ascending center in the anuran's central auditory nervous system. 2. Of the 142 cells isolated, 75 units responded only to monaural stimulation. Approximately 80% of these monaural cells could be excited by the ipsilateral ear, while the remaining 20% received their excitatory input from the contralateral ear. The other 67 units responded to binaural stimuli. Of these binaural cells, 14 could be excited by either contralateral or ipsilateral stimuli, and the threshold and best excitatory frequency were similar for each ear (EE). The other 53 binaural cells (EI) could be excited by stimulation of one ear and inhibited by stimulation of the other ear; for almost all of these cells the contralateral ear was excitatory and the ipsilateral ear was inhibitory. The best inhibitory frequency for one ear was approximately the same as the best excitatory frequency for the other ear, and the threshold for inhibition was near the threshold for excitation. 3. The tuning curves for all of the cells in the dorsal medullary nucleus were unimodal with \"Q\" values ranging from 0.4 to 4. The excitatory thresholds were widely scattered between 22 and 115 dB SPL. 4. The distribution of best excitatory frequencies for the monaural cells comprised three groups: 200-300, 500-800, and 900-1,600 Hz. The best excitatory frequencies of the binaural cells were scattered over this entire range, with a broad peak around 200-800 Hz. 5. Approximately 80% of the cells in the dorsal nucleus responded tonically throughout the duration of an excitatory tone burst. The remaining 20% of the cells responded phasically during the transient stages of a tone burst over a wide intensity range. 6. Response latencies were compared for the two types of monaural cells to tones at their best exciatatory frequencies at 10 dB above threshold. The latencies for the contralaterally excitable cells were just a few milliseconds longer than the latencies for the ipsilaterally excitable cells. For binaural cells the latency for contralateral stimulation was only 1-2 ms longer than for ipsilateral stimulation. It was concluded that the contralateral input to the dorsal medullary nucleus is not of efferent descending origin from higher auditory centers. 7. All of the binaural EI cells were sensitive to small interaural intensity differences and many were also sensitive to minute interaural time differences. These cells likely play a role in localization of sounds of significance to anurans."}
{"id": "PMID:1085816", "title": "Transverse-section radionuclide scanning in cisternography.", "content": "By applying the technique of transverse-section radionuclide scanning to cisternography, the structure and relationships of the basal cisterns and other subarachnoid spaces of the brain can be visualized more clearly and with more detail than is possible with routine imaging techniques. The ability of this method to separate overlapping areas of radioactivity ensures improved definition of space-occupying processes within the basal cisterns. In the evaluation by cisternography of patients with hydrocephalus and dementia, the transverse-section images clearly separated various normal and abnormal patterns, whereas the routine cisternogram images were equivocal.", "contents": "Transverse-section radionuclide scanning in cisternography. By applying the technique of transverse-section radionuclide scanning to cisternography, the structure and relationships of the basal cisterns and other subarachnoid spaces of the brain can be visualized more clearly and with more detail than is possible with routine imaging techniques. The ability of this method to separate overlapping areas of radioactivity ensures improved definition of space-occupying processes within the basal cisterns. In the evaluation by cisternography of patients with hydrocephalus and dementia, the transverse-section images clearly separated various normal and abnormal patterns, whereas the routine cisternogram images were equivocal."}
{"id": "PMID:1085817", "title": "Gas-liquid chromatographic determination of vitamin D in multivitamin preparations containing excess amounts of vitamin E.", "content": "Gas-liquid chromatographic (GLC) determination of vitamin D in multivitamin preparations containing excess amounts of vitamin E (a more than 2,500 weight ratio of dl-alpha-tocopheryl acetate to vitamin D) was investigated and a simplified routine method was established because the method reported previously (1) could not be applied to such special preparations. After applying the unsaponifiable matters of a sample to a phosphate-treated alumina column chromatography prepared according to MULDER et. al. (2), the eluate was evaporated and the residue was subjected to thin-layer chromatography (TLC) and GLC. When this method was applied to model preparations made by mixing vitamin D2 and dl-alpha-tocopheryl acetate (excess amounts), good results were obtained. Since the results on a commercial multivitamin preparation containing excess amounts of vitamin E were also satisfactory, it was confirmed that the proposed method could be used for simplified routine determinations.", "contents": "Gas-liquid chromatographic determination of vitamin D in multivitamin preparations containing excess amounts of vitamin E. Gas-liquid chromatographic (GLC) determination of vitamin D in multivitamin preparations containing excess amounts of vitamin E (a more than 2,500 weight ratio of dl-alpha-tocopheryl acetate to vitamin D) was investigated and a simplified routine method was established because the method reported previously (1) could not be applied to such special preparations. After applying the unsaponifiable matters of a sample to a phosphate-treated alumina column chromatography prepared according to MULDER et. al. (2), the eluate was evaporated and the residue was subjected to thin-layer chromatography (TLC) and GLC. When this method was applied to model preparations made by mixing vitamin D2 and dl-alpha-tocopheryl acetate (excess amounts), good results were obtained. Since the results on a commercial multivitamin preparation containing excess amounts of vitamin E were also satisfactory, it was confirmed that the proposed method could be used for simplified routine determinations."}
{"id": "PMID:1085820", "title": "Induction of transporting sites in a sodium transporting epithelium.", "content": "1. Frogs (Rana temporaria) were bathed for 1 week in solutions containing 1-1 mM sodium chloride and either one or both of amiloride (10(-4)M) and spironolactone (10(-5r both of amiloride (10(-4) M) and spironolactone (10(-5) M). This procedure was designed to deplete the sodium transporting compartment of the skin epithelium of sodium, while at the same time antagonizing the effects of endogenous aldosterone. 2. After 1 week the skins were used in vitro to measure the level of sodium transport (short-circuit current) and the density of sodium entry sites in the mucosal surface of the epithelium ([14C]amiloride binding). 3. Sodium deprivation for 1 week caused approximately a doubling of both sodium transport and the density of sodium entry sites in the mucosal surface of the epithelium compared to control skins. 4. When the results for sodium deprived and control skins were pooled there was a highly significant correlation between the density of sodium entry sites and sodium transport. 5. Mechanisms by which sodium deprivation leads to an increase in the density of sodium entry sites are discussed.", "contents": "Induction of transporting sites in a sodium transporting epithelium. 1. Frogs (Rana temporaria) were bathed for 1 week in solutions containing 1-1 mM sodium chloride and either one or both of amiloride (10(-4)M) and spironolactone (10(-5r both of amiloride (10(-4) M) and spironolactone (10(-5) M). This procedure was designed to deplete the sodium transporting compartment of the skin epithelium of sodium, while at the same time antagonizing the effects of endogenous aldosterone. 2. After 1 week the skins were used in vitro to measure the level of sodium transport (short-circuit current) and the density of sodium entry sites in the mucosal surface of the epithelium ([14C]amiloride binding). 3. Sodium deprivation for 1 week caused approximately a doubling of both sodium transport and the density of sodium entry sites in the mucosal surface of the epithelium compared to control skins. 4. When the results for sodium deprived and control skins were pooled there was a highly significant correlation between the density of sodium entry sites and sodium transport. 5. Mechanisms by which sodium deprivation leads to an increase in the density of sodium entry sites are discussed."}
{"id": "PMID:1085821", "title": "[The effect of ATP and acetylcholine on the sodium transport in bullfrog large intestine (author's transl)].", "content": "By Ussing's flux chamber method the effect of ATP and acetylcholine (ACh) on the sodium transport was studied in bullfrog colon. The results obtained are as follows; 1. ATP added to the mucosal medium caused biphasic changes in the transmural potential difference (P.D.) and short-circuit current (S.C.C.), although serosal ATP was ineffective. After an initial rapid and transient rise, both the P.D. and S.C.C. increased in parallel to reach a peak in about 10 min suggesting that the tissue conductance is little affected by ATP. Addition of ouabain to the serosal fluid depressed both the P.D. and S.C.C. and abolished the electrical responses to ATP. The application of ouabain to the mucosal side did not cause any significant depression. These results can be explained in terms of stimulation of sodium pump by ATP added to the mucosal medium. 2. ACh added to either the mucosal or the serosal medium caused increased in the P.D. and the S.C.C. The serosal application was more effective than the mucosal application. The increase in S.C.C. was more remarkable than that in the P.D., indicating an increase in the tissue conductance. It is suggested that ACh stimulates ion transport systems by changing the membrane permeability of the colon.", "contents": "[The effect of ATP and acetylcholine on the sodium transport in bullfrog large intestine (author's transl)]. By Ussing's flux chamber method the effect of ATP and acetylcholine (ACh) on the sodium transport was studied in bullfrog colon. The results obtained are as follows; 1. ATP added to the mucosal medium caused biphasic changes in the transmural potential difference (P.D.) and short-circuit current (S.C.C.), although serosal ATP was ineffective. After an initial rapid and transient rise, both the P.D. and S.C.C. increased in parallel to reach a peak in about 10 min suggesting that the tissue conductance is little affected by ATP. Addition of ouabain to the serosal fluid depressed both the P.D. and S.C.C. and abolished the electrical responses to ATP. The application of ouabain to the mucosal side did not cause any significant depression. These results can be explained in terms of stimulation of sodium pump by ATP added to the mucosal medium. 2. ACh added to either the mucosal or the serosal medium caused increased in the P.D. and the S.C.C. The serosal application was more effective than the mucosal application. The increase in S.C.C. was more remarkable than that in the P.D., indicating an increase in the tissue conductance. It is suggested that ACh stimulates ion transport systems by changing the membrane permeability of the colon."}
{"id": "PMID:1085824", "title": "Selective decrease in the rate of cleavage of an intracellular precursor to Rauscher leukemia virus p30 by treatment of infected cells with actinomycin D.", "content": "The cleavage  of an intracellular 67,000- to 70,000-dalton precursor, termed Pr4 to Rauscher leukemia virus (RLV) p30 protein proceeded at a slower rate when virus-producing cells were treated with actinomycin D (AMD). Treatment with AMD also caused a slight accumulation of Pr4 in purified early virus particles produced by a cell line which usually produces virions that contain little Pr4. The cleavage of other intracellular viral precursor polypeptides was not affected by treatment with AMD. Treatment of infected cells with cycloheximide, on the other hand, allowed the cleavage of Pr4 to proceed at the usual rate for a short period of time before further cleavage was drastically slowed or prevented. The cleavage of several other viral precursor polypeptides was also inhibited by treatment with cycloheximide. Different lines of evidence suggest that the mechanism of action of AMD is not due to a possible indirect effect on protein synthesis. Thus, the rate of cleavage of Pr4 was not affected by the length of pretreatment with AMD between 1 to 8 h. In addition, the combined effect of AMD and cycloheximide, at their maximal inhibitory concentrations, was greater than the effect of either drug alone, indicating the involvement of two at least partially different mechanisms in the action of AMD and cycloheximide. Furthermore, AMD did not affect the pulse labeling of viral precursor polypeptides. These results suggest that the interaction with viral RNA, whose production is inhibited by AMD, accelerates the cleavage of Pr4 to p30 during virus assembly. A hypothetical model is presented to illustrate th possible advantages of having a step in virus assembly in which genomic RNA interacts with a precursor to capsid proteins before the cleavage of that precursor.", "contents": "Selective decrease in the rate of cleavage of an intracellular precursor to Rauscher leukemia virus p30 by treatment of infected cells with actinomycin D. The cleavage  of an intracellular 67,000- to 70,000-dalton precursor, termed Pr4 to Rauscher leukemia virus (RLV) p30 protein proceeded at a slower rate when virus-producing cells were treated with actinomycin D (AMD). Treatment with AMD also caused a slight accumulation of Pr4 in purified early virus particles produced by a cell line which usually produces virions that contain little Pr4. The cleavage of other intracellular viral precursor polypeptides was not affected by treatment with AMD. Treatment of infected cells with cycloheximide, on the other hand, allowed the cleavage of Pr4 to proceed at the usual rate for a short period of time before further cleavage was drastically slowed or prevented. The cleavage of several other viral precursor polypeptides was also inhibited by treatment with cycloheximide. Different lines of evidence suggest that the mechanism of action of AMD is not due to a possible indirect effect on protein synthesis. Thus, the rate of cleavage of Pr4 was not affected by the length of pretreatment with AMD between 1 to 8 h. In addition, the combined effect of AMD and cycloheximide, at their maximal inhibitory concentrations, was greater than the effect of either drug alone, indicating the involvement of two at least partially different mechanisms in the action of AMD and cycloheximide. Furthermore, AMD did not affect the pulse labeling of viral precursor polypeptides. These results suggest that the interaction with viral RNA, whose production is inhibited by AMD, accelerates the cleavage of Pr4 to p30 during virus assembly. A hypothetical model is presented to illustrate th possible advantages of having a step in virus assembly in which genomic RNA interacts with a precursor to capsid proteins before the cleavage of that precursor."}
{"id": "PMID:1085830", "title": "Microelectrode study of spreading depression (SD) in frog retina--general observations of field potential associated with SD.", "content": "Using microelectrodes, the field potential change associated with SD (SDP) was recorded from frog retinas conditioned with Cl-free Ringer's. In such retinas, SDP was induced by light or chemical agents such as glutamate, aspartate and K+. The chemicals, when applied iontophoretically, produced a local graded response which eventually triggered SDP. A potential similar to the local response to chemicals was often discerned on the rising phase of SDPs produced by light or occurring spontaneously. The SDP was maximal across an innermost retinal layer 50 mum or less in thickness with the intraretinal polarity predominantly negative, indicating that the major sink of SDP is in the inner plexiform layer. The influence of SDP on the receptor potential was relatively small, but the other components completely disappeared at the beginning of SDP, recovering gradually thereafter. Concomitantly, a strong depolarization occurred in the ganglion cells. Stimulation of the optic nerve could induce SDP, but nerve impulse activity is not important for SDP because tetrodotoxin was unable to prevent SDP due to light or chemical agents.", "contents": "Microelectrode study of spreading depression (SD) in frog retina--general observations of field potential associated with SD. Using microelectrodes, the field potential change associated with SD (SDP) was recorded from frog retinas conditioned with Cl-free Ringer's. In such retinas, SDP was induced by light or chemical agents such as glutamate, aspartate and K+. The chemicals, when applied iontophoretically, produced a local graded response which eventually triggered SDP. A potential similar to the local response to chemicals was often discerned on the rising phase of SDPs produced by light or occurring spontaneously. The SDP was maximal across an innermost retinal layer 50 mum or less in thickness with the intraretinal polarity predominantly negative, indicating that the major sink of SDP is in the inner plexiform layer. The influence of SDP on the receptor potential was relatively small, but the other components completely disappeared at the beginning of SDP, recovering gradually thereafter. Concomitantly, a strong depolarization occurred in the ganglion cells. Stimulation of the optic nerve could induce SDP, but nerve impulse activity is not important for SDP because tetrodotoxin was unable to prevent SDP due to light or chemical agents."}
{"id": "PMID:1085831", "title": "Microelectrode study of spreading depression (SD) in frog retina-M\u00fcller cell activity and [K+] during SD--.", "content": "By means of K+ microelectrodes, K+ potentials and [K+] were studied in frog retinas conditioned for SD by CL--free Ringer's. A marked increase in [K+]o was observed during SD, the increase being maximal in the inner plexiform layer (up to 50 mM or more) and subsiding towards the distal retina. The extracellular K+ potential change during SD and also the graded K+ potential changes produced locally by iontophoretic injection of SD-stimulant chemicals resembled the membrane potential changes in M\u00fcller cells under the same conditions, suggesting that the M\u00fcller cells act as K+ electrodes. In retinas conditioned by Cl--free Ringer's M\u00fcller cells were swollen. This allowed intracellular recording with K+ electrodes in M\u00fcller cells to reveal that upon SD the M\u00fcller cells immediately start to cleanse the extracellular space of excess K+ which is probably a product of pathologically enhanced synaptic activity in the inner plexiform layer. The mechanism of SDP, the field potential change associated with SD, is discussed from a proposed model.", "contents": "Microelectrode study of spreading depression (SD) in frog retina-M\u00fcller cell activity and [K+] during SD--. By means of K+ microelectrodes, K+ potentials and [K+] were studied in frog retinas conditioned for SD by CL--free Ringer's. A marked increase in [K+]o was observed during SD, the increase being maximal in the inner plexiform layer (up to 50 mM or more) and subsiding towards the distal retina. The extracellular K+ potential change during SD and also the graded K+ potential changes produced locally by iontophoretic injection of SD-stimulant chemicals resembled the membrane potential changes in M\u00fcller cells under the same conditions, suggesting that the M\u00fcller cells act as K+ electrodes. In retinas conditioned by Cl--free Ringer's M\u00fcller cells were swollen. This allowed intracellular recording with K+ electrodes in M\u00fcller cells to reveal that upon SD the M\u00fcller cells immediately start to cleanse the extracellular space of excess K+ which is probably a product of pathologically enhanced synaptic activity in the inner plexiform layer. The mechanism of SDP, the field potential change associated with SD, is discussed from a proposed model."}
{"id": "PMID:1085832", "title": "Corynebacterium vaginale (Hemophilus vaginalis) bacteremia: clinical study of 29 cases.", "content": "Twenty-nine patients with bacteremia due to Corynebacterium vaginale, an inhabitant of the female genital tract, are described. Four were newborn babies. Nineteen were healthy young women delivered at full term by an operative procedure, cesarean section, or episiotomy. Within 48 hours fever and bacteremia developed. While receiving antibiotics the fever returned to normal, usually within 48 hours. The remaining cases were associated with septic abortion, tubal pregnancy, an intrauterine device, hydatidiform mole, and cellulitis. None of the adults showed evidence of brain abscess, meningitis, or endocarditis. Corynebacterium vaginale is an opportunistic minor pathogen that apparently gains access to the blood stream via an exposed vascular bed rather than as the result of immunosupression.", "contents": "Corynebacterium vaginale (Hemophilus vaginalis) bacteremia: clinical study of 29 cases. Twenty-nine patients with bacteremia due to Corynebacterium vaginale, an inhabitant of the female genital tract, are described. Four were newborn babies. Nineteen were healthy young women delivered at full term by an operative procedure, cesarean section, or episiotomy. Within 48 hours fever and bacteremia developed. While receiving antibiotics the fever returned to normal, usually within 48 hours. The remaining cases were associated with septic abortion, tubal pregnancy, an intrauterine device, hydatidiform mole, and cellulitis. None of the adults showed evidence of brain abscess, meningitis, or endocarditis. Corynebacterium vaginale is an opportunistic minor pathogen that apparently gains access to the blood stream via an exposed vascular bed rather than as the result of immunosupression."}
{"id": "PMID:1085834", "title": "[Nature of changes in the sympathico-adrenal system and certain indicators of homeostasis in patients with chronic coronary insufficiency during the process of surgical intervention].", "content": "A study of some aspects of homeostasis and hemodynamics in surgical interventions for chronic coronary insufficiency helped reveal a difference in the nature of their changes depending upon the severity of the course and outcomes of the operation. With the latter running an uneventful course there is observed, as a rule, a moderate rise of the blood catecholamines level and their abundant passage with urine, which appears as a prognostically favourable sign. In operations complicated by the \"syndrome of low cardiac ejection\" terminating lethally there was definable, on the contrary, a high blood catecholamines content in conjunction with their reduced passage with urine. This was considered a prognostically adverse sign.", "contents": "[Nature of changes in the sympathico-adrenal system and certain indicators of homeostasis in patients with chronic coronary insufficiency during the process of surgical intervention]. A study of some aspects of homeostasis and hemodynamics in surgical interventions for chronic coronary insufficiency helped reveal a difference in the nature of their changes depending upon the severity of the course and outcomes of the operation. With the latter running an uneventful course there is observed, as a rule, a moderate rise of the blood catecholamines level and their abundant passage with urine, which appears as a prognostically favourable sign. In operations complicated by the \"syndrome of low cardiac ejection\" terminating lethally there was definable, on the contrary, a high blood catecholamines content in conjunction with their reduced passage with urine. This was considered a prognostically adverse sign."}
{"id": "PMID:1085835", "title": "[Various indicators of the kinin and sympathico-adrenal systems in patients with recurrent myocardial infarct].", "content": "The interrelationship between the kinin and sympathoadrenal systems of blood was studied in 45 patients with repeated myocardial infarctions of different clinical course. Complicated repeated infarctions with rhythm disorders, cardiac asthma, pulmonary oedema, cardiogenic shock were accompanied by a more distinct elevation of the blood catecholamines level and the kinin system activity, than the non-complicated ones. The changes in the activity of the kinin and sympathoadrenal systems were found to parallel each other. Some diagnostic and more promising prognostic importance of the determined parameters was revealed.", "contents": "[Various indicators of the kinin and sympathico-adrenal systems in patients with recurrent myocardial infarct]. The interrelationship between the kinin and sympathoadrenal systems of blood was studied in 45 patients with repeated myocardial infarctions of different clinical course. Complicated repeated infarctions with rhythm disorders, cardiac asthma, pulmonary oedema, cardiogenic shock were accompanied by a more distinct elevation of the blood catecholamines level and the kinin system activity, than the non-complicated ones. The changes in the activity of the kinin and sympathoadrenal systems were found to parallel each other. Some diagnostic and more promising prognostic importance of the determined parameters was revealed."}
{"id": "PMID:1085840", "title": "[The clinical picture of metastatic myotic endophthalmitis (author's transl)].", "content": "Mycotic Septicaemia (especially with the Candida species) is not an uncommon hazard of hospitalized patients, especially those on intravenous hyperalimentation. Two such patients with endogenous mycotic bilateral endophthalmitis are presented. In spite of typical ocular symptoms diagnosis was delayed. Two further unilateral cases of a more atypical form of endogenous mycotic endophthalmitis in otherwise seemingly healthy patients are also described. Correlating histopathological findings in three of these 4 cases to the clinical histories, conclusions are drawn to aid an early diagnosis which is of paramount importance if the necessary antimyotic treatment is to preserve visual function.", "contents": "[The clinical picture of metastatic myotic endophthalmitis (author's transl)]. Mycotic Septicaemia (especially with the Candida species) is not an uncommon hazard of hospitalized patients, especially those on intravenous hyperalimentation. Two such patients with endogenous mycotic bilateral endophthalmitis are presented. In spite of typical ocular symptoms diagnosis was delayed. Two further unilateral cases of a more atypical form of endogenous mycotic endophthalmitis in otherwise seemingly healthy patients are also described. Correlating histopathological findings in three of these 4 cases to the clinical histories, conclusions are drawn to aid an early diagnosis which is of paramount importance if the necessary antimyotic treatment is to preserve visual function."}
{"id": "PMID:1085841", "title": "[Legal security in the determination of the \"anomalous quotient\" (author's transl)].", "content": "By testing individuals as well as a group seasonal oscillations of the Anomaloscope's mixing screw regulation could be observed. For that reason further standardization of research methods is requested.", "contents": "[Legal security in the determination of the \"anomalous quotient\" (author's transl)]. By testing individuals as well as a group seasonal oscillations of the Anomaloscope's mixing screw regulation could be observed. For that reason further standardization of research methods is requested."}
{"id": "PMID:1085842", "title": "[On the so-called inferior, sickle-shaped edematous corneal dystrophy (author's transl)].", "content": "The inferior, sickle-shaped, edematous corneal dystrophy described by Bietti has not been further reported in the literature. A similar corneal lesion in a 44-year-old patient provided the stimulus to express some thoughts as to the etiology and pathogenesis of this form of dystrophy.", "contents": "[On the so-called inferior, sickle-shaped edematous corneal dystrophy (author's transl)]. The inferior, sickle-shaped, edematous corneal dystrophy described by Bietti has not been further reported in the literature. A similar corneal lesion in a 44-year-old patient provided the stimulus to express some thoughts as to the etiology and pathogenesis of this form of dystrophy."}
{"id": "PMID:1085843", "title": "[Histiocytosis-X of the orbit (author's transl)].", "content": "After reviewing the syndrome of histiocytosis X, the special problems of an attack on the eye are discussed with regard to clinical, histological and radiological findings. By means of two cases, all aspects important for the diagnosis and treatment of histiocytosis X are discussed with reference to the literature. A histologically verified histiocytosis X in the area of the orbita is described in an almost 2 year old girl and another girl aged 7 years and 3 months. In both cases, the disease began with an edema of the upper eyelid and swelling of the lateral orbital margin. The second case showed in addition the clinical symptoms of a dacryoadenitis. The X-ray pictures of the skull revealed a large triangular osseous lesion in the area of the orbital margins. Blood chemistry, hematological tests and the bonemarrow puncture did not yield any pathological findings. The diagnosis of histiozytosis X could be made only by a biopsy from the tumor area. The swelling in the area of the eyelids and the lateral orbital margin disappeared under cytostatic therapy with Vincristin and Prednison.", "contents": "[Histiocytosis-X of the orbit (author's transl)]. After reviewing the syndrome of histiocytosis X, the special problems of an attack on the eye are discussed with regard to clinical, histological and radiological findings. By means of two cases, all aspects important for the diagnosis and treatment of histiocytosis X are discussed with reference to the literature. A histologically verified histiocytosis X in the area of the orbita is described in an almost 2 year old girl and another girl aged 7 years and 3 months. In both cases, the disease began with an edema of the upper eyelid and swelling of the lateral orbital margin. The second case showed in addition the clinical symptoms of a dacryoadenitis. The X-ray pictures of the skull revealed a large triangular osseous lesion in the area of the orbital margins. Blood chemistry, hematological tests and the bonemarrow puncture did not yield any pathological findings. The diagnosis of histiozytosis X could be made only by a biopsy from the tumor area. The swelling in the area of the eyelids and the lateral orbital margin disappeared under cytostatic therapy with Vincristin and Prednison."}
{"id": "PMID:1085845", "title": "Histological findings in rabbits which died with symptoms of mucoid enteritis.", "content": "Histological and aetiological similarities between rabbit mucoid enteritis and human ulcerative colitis are briefly discussed. Escherichia coli seems to be associated with the rabbit disease, and treatment aimed at this organism has been followed by a period of 4 years free from mucoid enteritis.", "contents": "Histological findings in rabbits which died with symptoms of mucoid enteritis. Histological and aetiological similarities between rabbit mucoid enteritis and human ulcerative colitis are briefly discussed. Escherichia coli seems to be associated with the rabbit disease, and treatment aimed at this organism has been followed by a period of 4 years free from mucoid enteritis."}
{"id": "PMID:1085849", "title": "Information factors affecting problem follow-up in ambulatory care.", "content": "The influence of information factors on the follow-up of patient problems was studied in six ambulatory clinics providing primary patient care. By means of chart review, the encounter notes were examined for two consecutive visits to the same clinic to determine problems identified at the first visit and detect evidence of follow-up of these problems at the second visit. In those clinics using a problem list, at the front of the chart, those problems on the problem list had a higher follow-up rate than those not on the list particularly at longer intervals between patient visits. An encounter note written in the problem-oriented format did not enhance follow-up. Since information factors as well as clinical factors affect the follow-up of patient problems these factors must be carefully considered when designing information systems to serve ambulatory care.", "contents": "Information factors affecting problem follow-up in ambulatory care. The influence of information factors on the follow-up of patient problems was studied in six ambulatory clinics providing primary patient care. By means of chart review, the encounter notes were examined for two consecutive visits to the same clinic to determine problems identified at the first visit and detect evidence of follow-up of these problems at the second visit. In those clinics using a problem list, at the front of the chart, those problems on the problem list had a higher follow-up rate than those not on the list particularly at longer intervals between patient visits. An encounter note written in the problem-oriented format did not enhance follow-up. Since information factors as well as clinical factors affect the follow-up of patient problems these factors must be carefully considered when designing information systems to serve ambulatory care."}
{"id": "PMID:1085850", "title": "Format review: evaluating implementation of the problem-oriented medical record.", "content": "As interest in the problem-oriented medical record (POMR) develops, and more medical settings begin to implement the system, many observers are expressing a desire to know how well the system is being used and accepted. Moreover, settings currently using the POMR system have a need to document the effectiveness of their POMR program as a means of insuring continued progress and for addressing difficulties and obstacles affecting its use. Format review is a technique for reviewing patient records and describing patterns of POMR use. Patient records of an institution are reviewed concurrent to the patient's contact with the institution. Data collected from each record is summarized to provide an overview on patterns of POMR use and to identify areas of difficulty. These findings can serve as the basis for educational and administrative intervention to improve use of POMR. The format review technique is explained and examples of its application are given. The technique has been used at Michael Reese Hospital and Medical Center over the past two years, and has proven to be a useful tool in describing the implementation progress of POMR.", "contents": "Format review: evaluating implementation of the problem-oriented medical record. As interest in the problem-oriented medical record (POMR) develops, and more medical settings begin to implement the system, many observers are expressing a desire to know how well the system is being used and accepted. Moreover, settings currently using the POMR system have a need to document the effectiveness of their POMR program as a means of insuring continued progress and for addressing difficulties and obstacles affecting its use. Format review is a technique for reviewing patient records and describing patterns of POMR use. Patient records of an institution are reviewed concurrent to the patient's contact with the institution. Data collected from each record is summarized to provide an overview on patterns of POMR use and to identify areas of difficulty. These findings can serve as the basis for educational and administrative intervention to improve use of POMR. The format review technique is explained and examples of its application are given. The technique has been used at Michael Reese Hospital and Medical Center over the past two years, and has proven to be a useful tool in describing the implementation progress of POMR."}
{"id": "PMID:1085851", "title": "[Report of 125 esophageal carcinomas (author's transl)].", "content": "This report concerns our experience in the treatment of 125 patients with carcinoma of the esophagus during 17 years (excluding the cardiac region of the stomach). In our material predisponsing factors had no effect upon the age of the patients. The symptomatology is described, the causes of postoperative complications and mortality are discussed. The principal indications are mentioned and technical considerations are summariced. Radical tumor resection resulted in a 2 year survival rate of 23 percent. Palliativ resections in cases of advanced carcinoma are refused because of the high mortality rate and the small expectation of life.", "contents": "[Report of 125 esophageal carcinomas (author's transl)]. This report concerns our experience in the treatment of 125 patients with carcinoma of the esophagus during 17 years (excluding the cardiac region of the stomach). In our material predisponsing factors had no effect upon the age of the patients. The symptomatology is described, the causes of postoperative complications and mortality are discussed. The principal indications are mentioned and technical considerations are summariced. Radical tumor resection resulted in a 2 year survival rate of 23 percent. Palliativ resections in cases of advanced carcinoma are refused because of the high mortality rate and the small expectation of life."}
{"id": "PMID:1085852", "title": "[Technique of submucous sclerosing in patients with bleeding esophageal varices -- therapeutic results (author's transl)].", "content": "Acute hemorrhage from esophageal varices is becoming more and more frequent, implying a poor prognosis for the patient and necessitating dramatic therapeutic procedures by the physician or the surgeon. Regular endoscopic controls of patients with liver cirrhosis and portal hypertension are necessary before the first bleeding occurs in spite of negative X-ray findings of the esophagus, since long-term therapeutic results may be inproved by such controls. Sclerosing the esophageal wall does not occlude the esophageal veins, which do remain open consequently; they are just \"displaced\" deeper into the mucosa. There are no risks or complications of this procedure especially when esophageal varices are just beginning to form and when the esophageal mucosa is still being intact. Therapeutic results are encouraging, and they are better as compared to the results of emergency shunt or elective shunt surgery. Until short time ago sclerosing the esophageal wall was sort of a preliminary preoperative therapeutic procedure. Today it is a well established therapy in its own right, although it is, as is shunt surgery, symptomatic therapy. It does seem to be superior however to all other forms of therapy aimed at preventing or treating esophageal bleeding, since primary mortality seems to be lower and longterm results seem to be better.", "contents": "[Technique of submucous sclerosing in patients with bleeding esophageal varices -- therapeutic results (author's transl)]. Acute hemorrhage from esophageal varices is becoming more and more frequent, implying a poor prognosis for the patient and necessitating dramatic therapeutic procedures by the physician or the surgeon. Regular endoscopic controls of patients with liver cirrhosis and portal hypertension are necessary before the first bleeding occurs in spite of negative X-ray findings of the esophagus, since long-term therapeutic results may be inproved by such controls. Sclerosing the esophageal wall does not occlude the esophageal veins, which do remain open consequently; they are just \"displaced\" deeper into the mucosa. There are no risks or complications of this procedure especially when esophageal varices are just beginning to form and when the esophageal mucosa is still being intact. Therapeutic results are encouraging, and they are better as compared to the results of emergency shunt or elective shunt surgery. Until short time ago sclerosing the esophageal wall was sort of a preliminary preoperative therapeutic procedure. Today it is a well established therapy in its own right, although it is, as is shunt surgery, symptomatic therapy. It does seem to be superior however to all other forms of therapy aimed at preventing or treating esophageal bleeding, since primary mortality seems to be lower and longterm results seem to be better."}
{"id": "PMID:1085857", "title": "A family with congenital deutan and tritan defects.", "content": "A family has been found with deuteranopia and a tritan defect which is not sex-linked. It is proposed that there is also an autosomal dominant gene for tritan defects showing variable expressivity.", "contents": "A family with congenital deutan and tritan defects. A family has been found with deuteranopia and a tritan defect which is not sex-linked. It is proposed that there is also an autosomal dominant gene for tritan defects showing variable expressivity."}
{"id": "PMID:1085859", "title": "Colour vision deficiencies and haemophilia.", "content": "Our investigations have following results: In the Rath-von Verschuer family, which is so extraordinary that the authenticity sometimes had been doubted, we found that crossing overs which were present in 50% of the cases were shown by the combination of protan defect and haemophilia B. This corresponds exactly to the observation of Whittaker and co-workers, who starting from that fact, concluded that there was a remarkable distance between the protan gene and haemophilia B gene on the chromosome. The observation enlarges the number of pedigrees with the combination of protan defect and haemophilia B. At the same time, however, we are doubtful that there might be a probable preference for this combination. With regard to the question whether manifestation of protanopia and protanomaly in any given form could be influenced by an additional factor in the X-chromosome, our present investigations give no reliable data.", "contents": "Colour vision deficiencies and haemophilia. Our investigations have following results: In the Rath-von Verschuer family, which is so extraordinary that the authenticity sometimes had been doubted, we found that crossing overs which were present in 50% of the cases were shown by the combination of protan defect and haemophilia B. This corresponds exactly to the observation of Whittaker and co-workers, who starting from that fact, concluded that there was a remarkable distance between the protan gene and haemophilia B gene on the chromosome. The observation enlarges the number of pedigrees with the combination of protan defect and haemophilia B. At the same time, however, we are doubtful that there might be a probable preference for this combination. With regard to the question whether manifestation of protanopia and protanomaly in any given form could be influenced by an additional factor in the X-chromosome, our present investigations give no reliable data."}
{"id": "PMID:1085861", "title": "A consideration of the racial incidence of congenital dyschromats in males and females.", "content": "The female incidence of congenital dyschromats corresponds to the square of the males in the Northern European populations, but this relation is not always true in the non-white races. The above-mentioned facts in the non-white races which are theoretically strange are considered to be caused by an anti-glare factor of pigment epithelium in their eyes, on the assumption that the abnormal color sense of color defectives may be a special form of visual dysfunction glare pointed out by IINUMA.", "contents": "A consideration of the racial incidence of congenital dyschromats in males and females. The female incidence of congenital dyschromats corresponds to the square of the males in the Northern European populations, but this relation is not always true in the non-white races. The above-mentioned facts in the non-white races which are theoretically strange are considered to be caused by an anti-glare factor of pigment epithelium in their eyes, on the assumption that the abnormal color sense of color defectives may be a special form of visual dysfunction glare pointed out by IINUMA."}
{"id": "PMID:1085863", "title": "Data concerning colour vision deficiencies amongst 29,985 young Greeks.", "content": "In this survey, 29,985 young Greek employees were screened by the Ishihara plates and if necessary by further more detailed methods (including anomaloscope). The examination of 21,231 young males revealed 212 (1.00%) protanopes, 241 (1.14%) deuteranopes, 254 (1.20%) protanomalous, 978 (4.61%) deuteranomalous and 2 with monochromatic vision. In total, 1,678 males (7.95%) had a colour vision disturbance. The examination of 8,754 young females revealed 1 (0.01%) protanope, 2 (0.02%) deuteranope, 3 (0.03%) protanomalous and 31 (0.35%) deuteranomalous; no case of monochromatic vision in females was detected. In total, only 37 (0.42%) females had a detectable colour vision disturbance with applied screening method. These results are discussed and compaired with the available bibliographic data.", "contents": "Data concerning colour vision deficiencies amongst 29,985 young Greeks. In this survey, 29,985 young Greek employees were screened by the Ishihara plates and if necessary by further more detailed methods (including anomaloscope). The examination of 21,231 young males revealed 212 (1.00%) protanopes, 241 (1.14%) deuteranopes, 254 (1.20%) protanomalous, 978 (4.61%) deuteranomalous and 2 with monochromatic vision. In total, 1,678 males (7.95%) had a colour vision disturbance. The examination of 8,754 young females revealed 1 (0.01%) protanope, 2 (0.02%) deuteranope, 3 (0.03%) protanomalous and 31 (0.35%) deuteranomalous; no case of monochromatic vision in females was detected. In total, only 37 (0.42%) females had a detectable colour vision disturbance with applied screening method. These results are discussed and compaired with the available bibliographic data."}
{"id": "PMID:1085871", "title": "A new screening method for detecting colour vision deficiencies.", "content": "Diagnostic colour vision examinations are generally administered with the anomaloscope. But this instrument requires a lot of time and experience for the tests as well as for the interpretation of the results. In practical use, it will be sufficient in most cases to know whether normal colour vision is present or not, and it is not necessary to use sophisticated devices. So it was considered desirable during the TCU conference on colour vision defects held in London in March 1973 [1] to have a testing method for practical use which is both accurate and time saving. At the time when this demand was brought up, a new colour vision test was being developed by Rodenstock [2]. It enables semi-skilled personnel to perform the test independently of ambient light condition in only 20-30 sec and to effect a classification of the type of colour vision at the same time [3].", "contents": "A new screening method for detecting colour vision deficiencies. Diagnostic colour vision examinations are generally administered with the anomaloscope. But this instrument requires a lot of time and experience for the tests as well as for the interpretation of the results. In practical use, it will be sufficient in most cases to know whether normal colour vision is present or not, and it is not necessary to use sophisticated devices. So it was considered desirable during the TCU conference on colour vision defects held in London in March 1973 [1] to have a testing method for practical use which is both accurate and time saving. At the time when this demand was brought up, a new colour vision test was being developed by Rodenstock [2]. It enables semi-skilled personnel to perform the test independently of ambient light condition in only 20-30 sec and to effect a classification of the type of colour vision at the same time [3]."}
{"id": "PMID:1085877", "title": "Visually evoked cortical potential (VECP) in dichromats.", "content": "The fundamental spectral sensitivity functions in man were studied by recording the visually evoked cortical potential (VECP). Using steadily presented strong blue-green and purple adaptation lights, two different spectral sensitivity functions were obtained with peaks in the green (540 nm) and in the red (590 nm) part of the spectrum. Only one peak (540 nm) was found in protanopia. The specific shift in deuteranopia and the influence of a blue-sensitive mechanism is discussed.", "contents": "Visually evoked cortical potential (VECP) in dichromats. The fundamental spectral sensitivity functions in man were studied by recording the visually evoked cortical potential (VECP). Using steadily presented strong blue-green and purple adaptation lights, two different spectral sensitivity functions were obtained with peaks in the green (540 nm) and in the red (590 nm) part of the spectrum. Only one peak (540 nm) was found in protanopia. The specific shift in deuteranopia and the influence of a blue-sensitive mechanism is discussed."}
{"id": "PMID:1085878", "title": "Experimental alteration of the red cone photoreceptor process.", "content": "In our experiments, intense monochromatic coherent light (647.1nm) was used to permanently alter the red cone process. As the turtle (Pseudemys) is very sensitive in the red region of the spectrum, it was chosen as an appropriate animal model in which to study initially experimentally induced protanopia. Dark adaptation and spectral sensitivity functions were measured for ERG criteria using a vector voltmeter. Successive dark adaptation functions were measured for chromatic light adaptation levels from full bleach to about two log units above full bleach, where the dark adaptation was abruptly altered in its dynamic range by about one log unit. Pre- and post-exposure measurements showed the action spectra suppressed in sensitivity by about one log unit from 600 to 660 nm. A small depression in the short wavelength region (420-500nm) was also observed.", "contents": "Experimental alteration of the red cone photoreceptor process. In our experiments, intense monochromatic coherent light (647.1nm) was used to permanently alter the red cone process. As the turtle (Pseudemys) is very sensitive in the red region of the spectrum, it was chosen as an appropriate animal model in which to study initially experimentally induced protanopia. Dark adaptation and spectral sensitivity functions were measured for ERG criteria using a vector voltmeter. Successive dark adaptation functions were measured for chromatic light adaptation levels from full bleach to about two log units above full bleach, where the dark adaptation was abruptly altered in its dynamic range by about one log unit. Pre- and post-exposure measurements showed the action spectra suppressed in sensitivity by about one log unit from 600 to 660 nm. A small depression in the short wavelength region (420-500nm) was also observed."}
{"id": "PMID:1085887", "title": "Unilateral colour vision defect resembling tritanopia.", "content": "A case of unilateral tritan defect is described. Colour-naming experiments showed that the tritanopic eye could perceive multiple colour hues. Although the defect resembled congenital tritanopia, it was considered to be acquired secondary to retinal pathology.", "contents": "Unilateral colour vision defect resembling tritanopia. A case of unilateral tritan defect is described. Colour-naming experiments showed that the tritanopic eye could perceive multiple colour hues. Although the defect resembled congenital tritanopia, it was considered to be acquired secondary to retinal pathology."}
{"id": "PMID:1085914", "title": "Mutation induction by MNNG in a bacteriophage of Haemophilus influenzae.", "content": "Three temperature-sensitive mutants of the Haemophilus influenzae phage HP1c1 were tested for reversion to wild type (ts leads to ts+). Treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) produced revertants at levels up to 0.1% of the total progeny phage from treated lysogens. Cells treated with MNNG after infection with whole ts phage produced progeny phage with similar reversion frequencies, but when the uninfected cells or the phage were treated alone no reversion was induced. Fixation of premutational lesions was shown to occur with no evidence for host-cell DNA synthesis, indicating that phage DNA synthesis may be responsible for fixation of mutation in phage DNA. Evidence is given which shows that prophage DNA replicating by the cells' replicating system after treatment and before induction, produces the same number of revertants per survivor as phage DNA which is replicated outside the host genome. Two of the phage mutants (ts1 and ts2) reverted at similar frequencies, while one of the mutants (ts3) exhibited a much lower induced reversion frequency.", "contents": "Mutation induction by MNNG in a bacteriophage of Haemophilus influenzae. Three temperature-sensitive mutants of the Haemophilus influenzae phage HP1c1 were tested for reversion to wild type (ts leads to ts+). Treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) produced revertants at levels up to 0.1% of the total progeny phage from treated lysogens. Cells treated with MNNG after infection with whole ts phage produced progeny phage with similar reversion frequencies, but when the uninfected cells or the phage were treated alone no reversion was induced. Fixation of premutational lesions was shown to occur with no evidence for host-cell DNA synthesis, indicating that phage DNA synthesis may be responsible for fixation of mutation in phage DNA. Evidence is given which shows that prophage DNA replicating by the cells' replicating system after treatment and before induction, produces the same number of revertants per survivor as phage DNA which is replicated outside the host genome. Two of the phage mutants (ts1 and ts2) reverted at similar frequencies, while one of the mutants (ts3) exhibited a much lower induced reversion frequency."}
{"id": "PMID:1085918", "title": "[Immunobiology of tuberculosis].", "content": "R. Koch showed in 1882 in his so-called fundamental experiment that tuberculous guinea pigs exhibit a vigorous immunity against re-infection. In investigations in 1930--40 we showed that this immunity is not mediated by humoral antibodies. It is a cellular process, a local and general activation of the macrophage system (the system of mononuclear phagocytes). The tuberculous granuloma consists of activated macrophages and their descendants (epitheloid and giant cells), which inhibit the growth and spreading of tubercle bacilli. The role of the specific and unspecific activation of the macrophage system could be demonstrated in experiments on rats, infected with Bartonella muris ratti. These early results are related to the recent knowledge concerning cellular immunity and granulomatous inflammation, especially to the cooperation between the lymphatic and macrophage system.", "contents": "[Immunobiology of tuberculosis]. R. Koch showed in 1882 in his so-called fundamental experiment that tuberculous guinea pigs exhibit a vigorous immunity against re-infection. In investigations in 1930--40 we showed that this immunity is not mediated by humoral antibodies. It is a cellular process, a local and general activation of the macrophage system (the system of mononuclear phagocytes). The tuberculous granuloma consists of activated macrophages and their descendants (epitheloid and giant cells), which inhibit the growth and spreading of tubercle bacilli. The role of the specific and unspecific activation of the macrophage system could be demonstrated in experiments on rats, infected with Bartonella muris ratti. These early results are related to the recent knowledge concerning cellular immunity and granulomatous inflammation, especially to the cooperation between the lymphatic and macrophage system."}
{"id": "PMID:1085920", "title": "[Rapid atrial stimulation in therapy of atrial flutter].", "content": "Personal experience in 12 cases of atrial flutter treated with various types of atrial electrostimulation is reported. In 6 cases the sinusal rhythm was obtained immediately. In the remaining cases sinusal rhythm was also obtained with atrial fibrillation from a minimum of 2 minutes to a maximum of 48 hours.", "contents": "[Rapid atrial stimulation in therapy of atrial flutter]. Personal experience in 12 cases of atrial flutter treated with various types of atrial electrostimulation is reported. In 6 cases the sinusal rhythm was obtained immediately. In the remaining cases sinusal rhythm was also obtained with atrial fibrillation from a minimum of 2 minutes to a maximum of 48 hours."}
{"id": "PMID:1085923", "title": "The widened spectrum of multiple cartilaginous exostosis (MCE).", "content": "2 brothers with possible homozygous multiple cartilaginous exostosis (MCE) are reported. The MCE-PD-(Peripheral Dysostosis) syndrome is discussed. A family (father, daughter and son) with Metachondromatosis is presented, and the tendency to spontaneous remission in this condition is emphasized. A \"second thought\", when considering the diagnosis of mce, seems worthwhile.", "contents": "The widened spectrum of multiple cartilaginous exostosis (MCE). 2 brothers with possible homozygous multiple cartilaginous exostosis (MCE) are reported. The MCE-PD-(Peripheral Dysostosis) syndrome is discussed. A family (father, daughter and son) with Metachondromatosis is presented, and the tendency to spontaneous remission in this condition is emphasized. A \"second thought\", when considering the diagnosis of mce, seems worthwhile."}
{"id": "PMID:1085924", "title": "Epiglottitis: incidence of extraepiglottic infection: report of 72 cases and review of the literature.", "content": "A review of 72 cases of epiglottitis seen at the Children's Hospital of Denver was undertaken to determine the incidence of extraepiglottic and septic foci in this disease. A parallel review of the literature was also undertaken. The clinical, bacteriologic, laboratory, and radiologic findings of this patient population are described. A 25% incidence of both pneumonia and cervical lymphadenitis was found to be associated with this illness. Exudative tonsillitis and otitis media were the only other complications, although they were infrequently noted. No cases of septic arthritis or meningitis were encountered, although 50% of these patients were recognized as bacteremic. A low incidence of septic complications (eight cases of meningitis and one case of periarticular abscess) is noted in a review of the literature. Recommendations for antibiotic management and definition of the population at risk for septic complications are given.", "contents": "Epiglottitis: incidence of extraepiglottic infection: report of 72 cases and review of the literature. A review of 72 cases of epiglottitis seen at the Children's Hospital of Denver was undertaken to determine the incidence of extraepiglottic and septic foci in this disease. A parallel review of the literature was also undertaken. The clinical, bacteriologic, laboratory, and radiologic findings of this patient population are described. A 25% incidence of both pneumonia and cervical lymphadenitis was found to be associated with this illness. Exudative tonsillitis and otitis media were the only other complications, although they were infrequently noted. No cases of septic arthritis or meningitis were encountered, although 50% of these patients were recognized as bacteremic. A low incidence of septic complications (eight cases of meningitis and one case of periarticular abscess) is noted in a review of the literature. Recommendations for antibiotic management and definition of the population at risk for septic complications are given."}
{"id": "PMID:1085927", "title": "Cleavage map of bacteriophage phiX174 RF DNA by restriction enzymes.", "content": "phiX RF DNA was cleaved by restriction enzymes from Haemophilus influenzae Rf (Hinf I) and Haemophilus haemolyticus (Hha. I). Twenty one fragments of approximately 25 to 730 base pairs were produced by Hinf I and seventeen fragments of approximately 40 to 1560 base pairs by Hha I. The order of these fragments has been established by digestion on Haemophilus awgyptius (Hae III) and Arthrobacter luteus (Alu I) endonuclease fragments of phiX RF with Hinf I and Hha1. By this method of reciprocal digestion a detailed cleavage map of phiX RF DNA was constructed, which includes also the previously determined Hind II, Hae III and Alu I cleavage maps of phiX 174 RF DNA (1, 2). Moreover, 28 conditional lethal mutants of bacteriophage phiX174 were placed in this map using the genetic fragment assay (3).", "contents": "Cleavage map of bacteriophage phiX174 RF DNA by restriction enzymes. phiX RF DNA was cleaved by restriction enzymes from Haemophilus influenzae Rf (Hinf I) and Haemophilus haemolyticus (Hha. I). Twenty one fragments of approximately 25 to 730 base pairs were produced by Hinf I and seventeen fragments of approximately 40 to 1560 base pairs by Hha I. The order of these fragments has been established by digestion on Haemophilus awgyptius (Hae III) and Arthrobacter luteus (Alu I) endonuclease fragments of phiX RF with Hinf I and Hha1. By this method of reciprocal digestion a detailed cleavage map of phiX RF DNA was constructed, which includes also the previously determined Hind II, Hae III and Alu I cleavage maps of phiX 174 RF DNA (1, 2). Moreover, 28 conditional lethal mutants of bacteriophage phiX174 were placed in this map using the genetic fragment assay (3)."}
{"id": "PMID:1085931", "title": "[Risks attendant on the use of electrical equipment in anaesthesia and intensive care units].", "content": "The physical principles of electricity and types of electrical injuries are reviewed. The extent of such injuries depends on the strength of the current: ventricular fibrillations can be induced by a current of 10mA, and even of 10 muA if the current passes directly through the heart. The extent of electrothermal injuries depends on the duration of the current flow and the contact area. Indirect electrical injuries may arise from interferance with cardiac pacemakers or monitoring equipment. Risks attendant on the use of even the \"safest\" electrical apparatus are discussed, with special reference to the risks arising from interaction between, e.g., high frequency diathermy and monitoring equipment or domestic appliances (beside lamp, razor) and monitoring or electrical therapeutic equipment. A number of safety measures to minimize the risk of electrical injuries are discussed.", "contents": "[Risks attendant on the use of electrical equipment in anaesthesia and intensive care units]. The physical principles of electricity and types of electrical injuries are reviewed. The extent of such injuries depends on the strength of the current: ventricular fibrillations can be induced by a current of 10mA, and even of 10 muA if the current passes directly through the heart. The extent of electrothermal injuries depends on the duration of the current flow and the contact area. Indirect electrical injuries may arise from interferance with cardiac pacemakers or monitoring equipment. Risks attendant on the use of even the \"safest\" electrical apparatus are discussed, with special reference to the risks arising from interaction between, e.g., high frequency diathermy and monitoring equipment or domestic appliances (beside lamp, razor) and monitoring or electrical therapeutic equipment. A number of safety measures to minimize the risk of electrical injuries are discussed."}
{"id": "PMID:1085934", "title": "Induction of splenic granulopoiesis in vitro.", "content": "Murine spleen cells were cultured in vitro to study the induction of committed granulopoietic stem cell (CFU-C) proliferation and maturation. Marbrook-type diffusion cultures were established with and without the addition of colony-stimulating activity (CSA) and harvested at intervals up to 14 days for viable and differential cell counts, [3H]TdR autoradiography, and quantitation of CFU-C by the agar plate method. Without CSA there was poor cell viability and little proliferative capacity. In CSA-stimulated cultures there was a prominent rise in viable cell counts and [3H]TdR labeling indices rose from a mean of 2% at 0 time to 47% after 5 days in vitro. CFU-C increased by 70-fold in these cultures. Peak numbers of CFU-C, immature cells, and [3H]TdR-labeled cells occurred at about 7 days. Thereafter, mature granulocytes and macrophages predominated in culture. Because the liquid spleen cell culture system begins in a resting state and undergoes a wave of proliferative activity in response to CSA, it can provide a useful model system for studying phenomena associated with stem cell activation and differentiation in vitro.", "contents": "Induction of splenic granulopoiesis in vitro. Murine spleen cells were cultured in vitro to study the induction of committed granulopoietic stem cell (CFU-C) proliferation and maturation. Marbrook-type diffusion cultures were established with and without the addition of colony-stimulating activity (CSA) and harvested at intervals up to 14 days for viable and differential cell counts, [3H]TdR autoradiography, and quantitation of CFU-C by the agar plate method. Without CSA there was poor cell viability and little proliferative capacity. In CSA-stimulated cultures there was a prominent rise in viable cell counts and [3H]TdR labeling indices rose from a mean of 2% at 0 time to 47% after 5 days in vitro. CFU-C increased by 70-fold in these cultures. Peak numbers of CFU-C, immature cells, and [3H]TdR-labeled cells occurred at about 7 days. Thereafter, mature granulocytes and macrophages predominated in culture. Because the liquid spleen cell culture system begins in a resting state and undergoes a wave of proliferative activity in response to CSA, it can provide a useful model system for studying phenomena associated with stem cell activation and differentiation in vitro."}
{"id": "PMID:1085935", "title": "Human lymphocyte subpopulations: the effect of pregnancy.", "content": "Peripheral blood lymphocytes from pregnant and nonpregnant females were studied for the presence of the following surface receptors: (i) Receptor for heat-aggregated human IgG (AggIgG); (ii) Receptor(s) for complement components; (iii) Receptor for sheep red blood cells (SRBC). Absolute numbers of lymphocytes with receptors for complement and those with receptors for SRBC were present in equal numbers in both groups. However, in the pregnant females there was a significantly lower number of lymphocytes with receptors for AggIgG. It is hypothesized that some of the immunological changes which occur during pregnancy may be mediated partly through changes in the total numbers of certain lymphocyte subpopulations.", "contents": "Human lymphocyte subpopulations: the effect of pregnancy. Peripheral blood lymphocytes from pregnant and nonpregnant females were studied for the presence of the following surface receptors: (i) Receptor for heat-aggregated human IgG (AggIgG); (ii) Receptor(s) for complement components; (iii) Receptor for sheep red blood cells (SRBC). Absolute numbers of lymphocytes with receptors for complement and those with receptors for SRBC were present in equal numbers in both groups. However, in the pregnant females there was a significantly lower number of lymphocytes with receptors for AggIgG. It is hypothesized that some of the immunological changes which occur during pregnancy may be mediated partly through changes in the total numbers of certain lymphocyte subpopulations."}
{"id": "PMID:1085936", "title": "Corticotropin releasing factor (CRF) activity in rat plasma.", "content": "The ACTH-releasing activity of hypothalamic extract and rat plasma was examined with the dispersed rat pituitary cell technique of Swallow and Sayer (8). Although both plasma and serum caused ACTH release which was dose-related, stress did not enhance the ACTH releasing activity. Furthermore, separation studies of plasma using ultrafiltration and gel separation suggest that the CRF activity in plasma is associated with molecules of a molecular weight greater than 15,000.", "contents": "Corticotropin releasing factor (CRF) activity in rat plasma. The ACTH-releasing activity of hypothalamic extract and rat plasma was examined with the dispersed rat pituitary cell technique of Swallow and Sayer (8). Although both plasma and serum caused ACTH release which was dose-related, stress did not enhance the ACTH releasing activity. Furthermore, separation studies of plasma using ultrafiltration and gel separation suggest that the CRF activity in plasma is associated with molecules of a molecular weight greater than 15,000."}
{"id": "PMID:1085937", "title": "Central monoamine deficiency in depressions: causative of secondary phenomenon?", "content": "There are indications for a functional deficiency of 5-HT and DA in certain kinds of depression. The question arises if these biochemical disturbances are primary or secondary, whether they contribute to the pathogenesis of the depression or whether they result from it. From research with MA precursors we drew the tentative conclusion that they are presumabely primary and interrelated with the depression in a causal and/or predisposing way.", "contents": "Central monoamine deficiency in depressions: causative of secondary phenomenon? There are indications for a functional deficiency of 5-HT and DA in certain kinds of depression. The question arises if these biochemical disturbances are primary or secondary, whether they contribute to the pathogenesis of the depression or whether they result from it. From research with MA precursors we drew the tentative conclusion that they are presumabely primary and interrelated with the depression in a causal and/or predisposing way."}
{"id": "PMID:1085940", "title": "The effects of intramyometrial injection of prostaglandin F2alpha on severe post-partum hemorrhage.", "content": "Effectiveness of prostaglandin F2alpha (PGF2alpha) in controlling postpartum uterine hemorrhage was evaluated with the following results. (1) Systemic administration, whether by continuous i.v. infusion or by gluteal i.m. injection, was not a completely adequate method for hemostasis. (2) Local administration by directly injecting into the uterine musculature, whether transabdominally or transvaginally, resulted in a dramatic reduction of the rate of bleeding. Routine clinical application of the direct intramyometrial injection of PGF2alpha for severe post-partum hemorrhage is recommended in view of its easy performance, excellent hemostatic effect, minimal side effects and good prognosis.", "contents": "The effects of intramyometrial injection of prostaglandin F2alpha on severe post-partum hemorrhage. Effectiveness of prostaglandin F2alpha (PGF2alpha) in controlling postpartum uterine hemorrhage was evaluated with the following results. (1) Systemic administration, whether by continuous i.v. infusion or by gluteal i.m. injection, was not a completely adequate method for hemostasis. (2) Local administration by directly injecting into the uterine musculature, whether transabdominally or transvaginally, resulted in a dramatic reduction of the rate of bleeding. Routine clinical application of the direct intramyometrial injection of PGF2alpha for severe post-partum hemorrhage is recommended in view of its easy performance, excellent hemostatic effect, minimal side effects and good prognosis."}
{"id": "PMID:1085963", "title": "[Electrical stimulation therapy and its effects on the general activity of motor impaired cerebral palsied children; a comparative study of the Bobath physiotherapy and its combination with the Hufschmidt electrical stimulation therapy (author's transl)].", "content": "The purpose of this study was to answer the following questions: (1) Is it more effective to treat spastic cerebral palsy with the Hufschmidt electrical stimulation therapy combined with the Bobath neuro-development treatment or only with the Bobath therapy? (2) Can a general increase in activity be obtained by the electrotherapeutic muscle stimulation? A test group (combined Hufschmidt/Bobath therapy) and a control group (Bobath), both consisting of 10 subjects, were observed for four months. The duration of observation was divided into two four months treatment periods with a rest interval of two months in between. At the start of therapeutic measures, motor activity and psychic condition were tested with corresponding motormetric and psychodiagnostic techniques; three check-up examinations were carried out at the end of the first, and at the beginning and end of the second period of treatment. The motor-metric control examination showed that at the end of the first period the test group had achieved by far the better results, but at the end of the second therapeutic period, both groups were equally successful. The combined electrophysiotherapy hence reached in a relatively shorter time - as it were by leaps and bounds - the optimal obtainable state of functional improvements which, with the Bobath therapy alone, can be effected more slowly but with more continuity. The psychodiagnostic controls clearly indicate that the electrical stimulation produced an unspecified increase in activity, especially after the first phase of treatment, whereas in the second phase this could only be proven in a graded form. The report closes with an examination of the results and their consequences for the implementation of the treatment for cerebral palsied children.", "contents": "[Electrical stimulation therapy and its effects on the general activity of motor impaired cerebral palsied children; a comparative study of the Bobath physiotherapy and its combination with the Hufschmidt electrical stimulation therapy (author's transl)]. The purpose of this study was to answer the following questions: (1) Is it more effective to treat spastic cerebral palsy with the Hufschmidt electrical stimulation therapy combined with the Bobath neuro-development treatment or only with the Bobath therapy? (2) Can a general increase in activity be obtained by the electrotherapeutic muscle stimulation? A test group (combined Hufschmidt/Bobath therapy) and a control group (Bobath), both consisting of 10 subjects, were observed for four months. The duration of observation was divided into two four months treatment periods with a rest interval of two months in between. At the start of therapeutic measures, motor activity and psychic condition were tested with corresponding motormetric and psychodiagnostic techniques; three check-up examinations were carried out at the end of the first, and at the beginning and end of the second period of treatment. The motor-metric control examination showed that at the end of the first period the test group had achieved by far the better results, but at the end of the second therapeutic period, both groups were equally successful. The combined electrophysiotherapy hence reached in a relatively shorter time - as it were by leaps and bounds - the optimal obtainable state of functional improvements which, with the Bobath therapy alone, can be effected more slowly but with more continuity. The psychodiagnostic controls clearly indicate that the electrical stimulation produced an unspecified increase in activity, especially after the first phase of treatment, whereas in the second phase this could only be proven in a graded form. The report closes with an examination of the results and their consequences for the implementation of the treatment for cerebral palsied children."}
{"id": "PMID:1085962", "title": "[Pharmacokinetics of benziodarone labelled with I-131].", "content": "Regarding the importance of benziodarone (Retrangor) -- 2-ethyl-3-(3',5'-diiodo-4'-hidrovibenzoyl)benzofuran -- in medical practic, in respect of its vasodilator and uricosuric action, a working plan was developed intending to study the biological distribution of the drug in rats Wistar and, using the plasmatic curve, to find out the existence of other compartements besides the intravascular one. With these data, the rhytms of benziodarone exchange were established, simulating its distribution in the organism. It was possible to develop this working project because the benziodarone structure could be labelled with iodine-131, using an isotopic exchange reaction, having chloramine T as an oxidant agent. The labelled compound was employed as radioactive tracer, and its specific activities varied from 18 uCi/mg to 20 uCi/mg.", "contents": "[Pharmacokinetics of benziodarone labelled with I-131]. Regarding the importance of benziodarone (Retrangor) -- 2-ethyl-3-(3',5'-diiodo-4'-hidrovibenzoyl)benzofuran -- in medical practic, in respect of its vasodilator and uricosuric action, a working plan was developed intending to study the biological distribution of the drug in rats Wistar and, using the plasmatic curve, to find out the existence of other compartements besides the intravascular one. With these data, the rhytms of benziodarone exchange were established, simulating its distribution in the organism. It was possible to develop this working project because the benziodarone structure could be labelled with iodine-131, using an isotopic exchange reaction, having chloramine T as an oxidant agent. The labelled compound was employed as radioactive tracer, and its specific activities varied from 18 uCi/mg to 20 uCi/mg."}
{"id": "PMID:1085985", "title": "Hearing loss as a sequel to chloramphenicol and ampicillin treatment of Haemophilus influenzae meningitis.", "content": "20 patients with Haemophilus influenzae meningitis who had been treated with chloramphenicol over the period 1959-1970 and 23 patients who had been treated with ampicillin over the period 1968-1974 were re-examined by hearing tests in 1975. In all the cases the two agents had been given initially by the parenteral route, chloramphenicol in doses varying between 50 and 150 (averaging 101) mg/kg/day and ampicillin in doses varying between 125 and 350 (averaging 229) mg/kg/day. Five of the 20 patients in the chloramphenicol group were found to be deaf on one ear, whereas 1 of the 23 patients in the ampicillin group was completely deaf. A further 3 in the chloramphenicol group and 1 in the ampicillin group had slight sensorineural hearing loss on one ear. Only in 2 of the 6 deaf patients was the loss discovered during the time of hospital care. The present study has not provided any evidence supporting the recently reported observation (Gamstorp and Klockhoff, 1974) that the frequency of hearing loss might be higher after ampicillin than after chloramphenicol treatment for H. influenzae meningitis.", "contents": "Hearing loss as a sequel to chloramphenicol and ampicillin treatment of Haemophilus influenzae meningitis. 20 patients with Haemophilus influenzae meningitis who had been treated with chloramphenicol over the period 1959-1970 and 23 patients who had been treated with ampicillin over the period 1968-1974 were re-examined by hearing tests in 1975. In all the cases the two agents had been given initially by the parenteral route, chloramphenicol in doses varying between 50 and 150 (averaging 101) mg/kg/day and ampicillin in doses varying between 125 and 350 (averaging 229) mg/kg/day. Five of the 20 patients in the chloramphenicol group were found to be deaf on one ear, whereas 1 of the 23 patients in the ampicillin group was completely deaf. A further 3 in the chloramphenicol group and 1 in the ampicillin group had slight sensorineural hearing loss on one ear. Only in 2 of the 6 deaf patients was the loss discovered during the time of hospital care. The present study has not provided any evidence supporting the recently reported observation (Gamstorp and Klockhoff, 1974) that the frequency of hearing loss might be higher after ampicillin than after chloramphenicol treatment for H. influenzae meningitis."}
{"id": "PMID:1085986", "title": "Potassium accumulation in muscle: a test of the binding hypothesis.", "content": "Living frog skeletal muscle can accumulate potassium in vitro to concentrations up to 580 millimolar. Both the amount of potassium accumulated and the relationship between intracellular and extracellular potassium concentrations indicate that potassium is \"free\" under all conditions, rather than bound to cellular macromolecules. The data also indicate that at most 20 percent of the cell water is \"bound\" in the sense that it excludes electrolytes.", "contents": "Potassium accumulation in muscle: a test of the binding hypothesis. Living frog skeletal muscle can accumulate potassium in vitro to concentrations up to 580 millimolar. Both the amount of potassium accumulated and the relationship between intracellular and extracellular potassium concentrations indicate that potassium is \"free\" under all conditions, rather than bound to cellular macromolecules. The data also indicate that at most 20 percent of the cell water is \"bound\" in the sense that it excludes electrolytes."}
{"id": "PMID:1085993", "title": "CSF enhancement for computerized tomography.", "content": "Metrizamide cisternography, combined with hypocycloidal tomography, has been performed in 12 patients with possible posterior fossa or parasellar mass lesions with finely detailed images of the basal cisterns resulting. Computed tomography was performed in nine of these patients and produced exceptional images of the cisternal anatomy. The technique of cerebrospinal fluid enhancement may be efficacious in identifying small basal masses not shown with conventional computed tomography, and thus may form an important complement to enhancement by intravenous injection of contrast medium.", "contents": "CSF enhancement for computerized tomography. Metrizamide cisternography, combined with hypocycloidal tomography, has been performed in 12 patients with possible posterior fossa or parasellar mass lesions with finely detailed images of the basal cisterns resulting. Computed tomography was performed in nine of these patients and produced exceptional images of the cisternal anatomy. The technique of cerebrospinal fluid enhancement may be efficacious in identifying small basal masses not shown with conventional computed tomography, and thus may form an important complement to enhancement by intravenous injection of contrast medium."}
{"id": "PMID:1085994", "title": "Total excision of the sternum and thoracic pedicle transposition of the greater omentum; useful strategems in managing severe mediastinal infection following open heart surgery.", "content": "Mediastinal sepsis following open heart surgery is a significant cause of death. Open drainage of the mediastinumalone was employed originally in management of this problem. More recently, debridement, drainage, and reclosure have been used. Various irrigation solutions, such as antibiotics and Betadine, have been advocated to control severe mediastinal sepsis. Three principles of management in patients unresponsiveness to the above techniques have proved successful in two patients with life-threatening mediastinal sepsis: (1) radical, complete excision of the sternum and adjacent costal cartilages; (2) transposition of the greater omentum on a vascular pedicle to the mediastinum; and (3) primary closure with full-thickness rotational skin flaps. The radical excision of the sternum removes residual foci of sepsis in cartilage and sternal bone marrow. The transposition of the omentum provides a highly vascular, rapidly granulating covering for the contaminated great vessels and hase been successfully to prevent recurrence of suture line bleeding of an exposed ascending aortic anastomosis site. Primary closure of the wound with full-thickness skin flaps provides a suprisingly satisfactory covering for the heart. Preoperative and postoperative measurements of ventilatory mechanics have shown relatively small ventilatory impairment after the alteration of the thoracic cage imposed by excision of the sternum. Two patients have returned to active lives. A treatment failure probably due to incomplete adherence to these guidelines also is presented.", "contents": "Total excision of the sternum and thoracic pedicle transposition of the greater omentum; useful strategems in managing severe mediastinal infection following open heart surgery. Mediastinal sepsis following open heart surgery is a significant cause of death. Open drainage of the mediastinumalone was employed originally in management of this problem. More recently, debridement, drainage, and reclosure have been used. Various irrigation solutions, such as antibiotics and Betadine, have been advocated to control severe mediastinal sepsis. Three principles of management in patients unresponsiveness to the above techniques have proved successful in two patients with life-threatening mediastinal sepsis: (1) radical, complete excision of the sternum and adjacent costal cartilages; (2) transposition of the greater omentum on a vascular pedicle to the mediastinum; and (3) primary closure with full-thickness rotational skin flaps. The radical excision of the sternum removes residual foci of sepsis in cartilage and sternal bone marrow. The transposition of the omentum provides a highly vascular, rapidly granulating covering for the contaminated great vessels and hase been successfully to prevent recurrence of suture line bleeding of an exposed ascending aortic anastomosis site. Primary closure of the wound with full-thickness skin flaps provides a suprisingly satisfactory covering for the heart. Preoperative and postoperative measurements of ventilatory mechanics have shown relatively small ventilatory impairment after the alteration of the thoracic cage imposed by excision of the sternum. Two patients have returned to active lives. A treatment failure probably due to incomplete adherence to these guidelines also is presented."}
{"id": "PMID:1085995", "title": "Surgical management of complicated acute coronary insufficiency.", "content": "Acute coronary insufficiency (ACI) has a one year mortality rate approximating 40 percent with medical treatment alone. This report reviews our experience over 24 months with preoperative intra-aortic balloon pump assist (IABPA) in 42 patients with ACI. Abnormal left ventricular (LV) hemodynamics were present in the majority of patients; the ejection fraction was less than 40 percent in 14 patients. The endocardial viability ratio (EVR) was less than 0.7 in eight patients. The mean coronary artery score was 13, compared to 9 in an otherwise comparable group of patients with stable angina. Left main coronary stenosis greater than 75 percent was present in seven patients and combined with significant stenosis (less than 72 percent) in the dominant right system in four patients. Four patients had proximal stenoses greater than 90 percent in all three major coronary arteries. IABPA was initiated in 11 patients prior to angiography because of refractory rest pain. One of these six patients died. Twenty-five other patients were supported before and six after induction of general anesthesia. Thirty-three of 36 revascularized patients survived. Of four patients with perioperative myocardial infarctions (12 percent), three had IABPA after induction of general anesthesia. Inotropic support and duration of stay both in intensive care and in the hospital were less than in similar patients treated before the use of IABPA.", "contents": "Surgical management of complicated acute coronary insufficiency. Acute coronary insufficiency (ACI) has a one year mortality rate approximating 40 percent with medical treatment alone. This report reviews our experience over 24 months with preoperative intra-aortic balloon pump assist (IABPA) in 42 patients with ACI. Abnormal left ventricular (LV) hemodynamics were present in the majority of patients; the ejection fraction was less than 40 percent in 14 patients. The endocardial viability ratio (EVR) was less than 0.7 in eight patients. The mean coronary artery score was 13, compared to 9 in an otherwise comparable group of patients with stable angina. Left main coronary stenosis greater than 75 percent was present in seven patients and combined with significant stenosis (less than 72 percent) in the dominant right system in four patients. Four patients had proximal stenoses greater than 90 percent in all three major coronary arteries. IABPA was initiated in 11 patients prior to angiography because of refractory rest pain. One of these six patients died. Twenty-five other patients were supported before and six after induction of general anesthesia. Thirty-three of 36 revascularized patients survived. Of four patients with perioperative myocardial infarctions (12 percent), three had IABPA after induction of general anesthesia. Inotropic support and duration of stay both in intensive care and in the hospital were less than in similar patients treated before the use of IABPA."}
{"id": "PMID:1085998", "title": "[Observation in 10 calves with a survival time of more than 100 hours after total heart replacement (author's transl)].", "content": "It is reported on 10 calves surviving total artificial heart replacement more than 100 hours. Pneumatically driven double-chambered blood pumps made of silastic were implanted. During the experiments an increase of the right atrial pressure as well as a rise of the perfusion volumes (CO) and of the blood volumes could be found regularly. At autopsy congestion of the organs especially of the liver was a regular finding. The changed hemodynamic is first seen as possible reason for this developments. The influence of anemia and the possible disturbances in the neurohumoral autoregulation of the animals by unphysiologic pressure curves are discussed.", "contents": "[Observation in 10 calves with a survival time of more than 100 hours after total heart replacement (author's transl)]. It is reported on 10 calves surviving total artificial heart replacement more than 100 hours. Pneumatically driven double-chambered blood pumps made of silastic were implanted. During the experiments an increase of the right atrial pressure as well as a rise of the perfusion volumes (CO) and of the blood volumes could be found regularly. At autopsy congestion of the organs especially of the liver was a regular finding. The changed hemodynamic is first seen as possible reason for this developments. The influence of anemia and the possible disturbances in the neurohumoral autoregulation of the animals by unphysiologic pressure curves are discussed."}
{"id": "PMID:1085999", "title": "[Silicone embolism, fat embolism, and fibrin thrombosis in dogs after extracorporeal circulation using a bubble-oxygenator (author's transl)].", "content": "Following extracorporeal circulation with a bubble oxygenator (Rygg-Kyvsgaard) silicone emboli were found in the brain-and-kidney-capillaries of all dogs evaluated. There was no obvious correlation between intensity of silicone embolism and pump time. No cellular reaction was seen around the anti-foam agent. Occasionally single small areas of embolic brain damage were found. During extracorporeal circulation and within a recovery period up to one hour no silicone excretion through the kidneys could be demonstrated. Systemic fat embolism occurred less frequent than previously reported. Use of a filter in the cardiotomy suction line reduced its intensity further. In neither of the various organs examined, disseminated intravascular thrombosis could be found.", "contents": "[Silicone embolism, fat embolism, and fibrin thrombosis in dogs after extracorporeal circulation using a bubble-oxygenator (author's transl)]. Following extracorporeal circulation with a bubble oxygenator (Rygg-Kyvsgaard) silicone emboli were found in the brain-and-kidney-capillaries of all dogs evaluated. There was no obvious correlation between intensity of silicone embolism and pump time. No cellular reaction was seen around the anti-foam agent. Occasionally single small areas of embolic brain damage were found. During extracorporeal circulation and within a recovery period up to one hour no silicone excretion through the kidneys could be demonstrated. Systemic fat embolism occurred less frequent than previously reported. Use of a filter in the cardiotomy suction line reduced its intensity further. In neither of the various organs examined, disseminated intravascular thrombosis could be found."}
{"id": "PMID:1086000", "title": "[Double mitral valve combined with an intermediate type of persistent atrioventricular canal. A contribution to surgical correction (author's transl)].", "content": "A case of double mitral valve combined with an intermediate type of atrioventricular canal is reported. After repairing the cleft in the aortic cusp of the mitral valve there resulted a mitral stenosis, which could be corrected by cutting the bridge between the two mitral orifices. In regarding the genesis of double mitral valve it seems possible, that there are two different types of this congenital malformation.", "contents": "[Double mitral valve combined with an intermediate type of persistent atrioventricular canal. A contribution to surgical correction (author's transl)]. A case of double mitral valve combined with an intermediate type of atrioventricular canal is reported. After repairing the cleft in the aortic cusp of the mitral valve there resulted a mitral stenosis, which could be corrected by cutting the bridge between the two mitral orifices. In regarding the genesis of double mitral valve it seems possible, that there are two different types of this congenital malformation."}
{"id": "PMID:1086004", "title": "[Anatomy and pathology of the tricuspid valve (author's transl)].", "content": "Anatomy and pathology of the tricuspid valve are presented in a short survey. Annulus fibrosus and leaflets are less firm than those of the mitral valve, whereas the tricuspid orifice is larger than the mitral orifice. Isolated tricuspid disease - congenital or acquired - occurs only rarely. Functional non organic tricuspid regurgitation is clinically of primary importance.", "contents": "[Anatomy and pathology of the tricuspid valve (author's transl)]. Anatomy and pathology of the tricuspid valve are presented in a short survey. Annulus fibrosus and leaflets are less firm than those of the mitral valve, whereas the tricuspid orifice is larger than the mitral orifice. Isolated tricuspid disease - congenital or acquired - occurs only rarely. Functional non organic tricuspid regurgitation is clinically of primary importance."}
{"id": "PMID:1086005", "title": "[Incidence and relevance of tricuspid-valve insufficiency in acquired mitral-valve defect. Analysis based on right ventricular angiograms].", "content": "To detect tricuspid incompetence (TI) right ventricular angiography was performed in 167 patients suffering from moderate to severe mitral valve disease. Holosystolic reflux of contrast medium to the right atrium through the central part of the tricuspid valve was thought to represent true TI, whereas a jet of contrast medium following the injection catheter and originating from the region of its valve passage was assumed to reflect arteficial regurgitation. True TI was found in 35% of the total group (30% mild to moderate, 5% severe TI). TI was often accompanied by atrial fibrillation (91%), pulmonary hypertension (74%) and reduced contraction of the tricuspid annulus (55%). Since tricuspid regurgitation in mitral valve disease commonly represents \"functional\" incompetence surgical intervention may be recommandable only in case of severe TI or concomitant valvular stenosis.", "contents": "[Incidence and relevance of tricuspid-valve insufficiency in acquired mitral-valve defect. Analysis based on right ventricular angiograms]. To detect tricuspid incompetence (TI) right ventricular angiography was performed in 167 patients suffering from moderate to severe mitral valve disease. Holosystolic reflux of contrast medium to the right atrium through the central part of the tricuspid valve was thought to represent true TI, whereas a jet of contrast medium following the injection catheter and originating from the region of its valve passage was assumed to reflect arteficial regurgitation. True TI was found in 35% of the total group (30% mild to moderate, 5% severe TI). TI was often accompanied by atrial fibrillation (91%), pulmonary hypertension (74%) and reduced contraction of the tricuspid annulus (55%). Since tricuspid regurgitation in mitral valve disease commonly represents \"functional\" incompetence surgical intervention may be recommandable only in case of severe TI or concomitant valvular stenosis."}
{"id": "PMID:1086006", "title": "[Surgical management of acquired tricuspid insufficiency--valvular replacement or annuloplasty? (author's transl)].", "content": "61 patient underwent operative repair of acquired tricuspid insufficiency (T.I.) during correction of multiple valvular disease. 45% presented a relative T.I. through annular dilatation, while organic lesions of the tricuspid valve were found in 56% of the patients. A tricuspid valvular replacement was carried out in 17, and a tricuspid annuloplasty in 44 patients. We believe that during surgical management of multiple valvular lesions, all tricuspid insufficiencies of even when only of a slight to moderate degree - should be corrected aggressively. Tricuspid valvular replacement should be avoided if possible and a tricuspid annuloplasty should be performed.", "contents": "[Surgical management of acquired tricuspid insufficiency--valvular replacement or annuloplasty? (author's transl)]. 61 patient underwent operative repair of acquired tricuspid insufficiency (T.I.) during correction of multiple valvular disease. 45% presented a relative T.I. through annular dilatation, while organic lesions of the tricuspid valve were found in 56% of the patients. A tricuspid valvular replacement was carried out in 17, and a tricuspid annuloplasty in 44 patients. We believe that during surgical management of multiple valvular lesions, all tricuspid insufficiencies of even when only of a slight to moderate degree - should be corrected aggressively. Tricuspid valvular replacement should be avoided if possible and a tricuspid annuloplasty should be performed."}
{"id": "PMID:1086007", "title": "[Measurement of cardiac output by impedance cardiography. Experimental studies (author's transl)].", "content": "The correlation of impedance cardiography against electromagnetic flow measurement was studied in the pig. The conditions tested were normal circulation, hypo- und hypervolemia and acute pulmonary hypertension as well as the influence of respiration and electrodes location. Correct quantification of cardiac output was obtained only in normovolemic circulation during exspiration (r = 0,96, m = 1,15, b = 0,04).", "contents": "[Measurement of cardiac output by impedance cardiography. Experimental studies (author's transl)]. The correlation of impedance cardiography against electromagnetic flow measurement was studied in the pig. The conditions tested were normal circulation, hypo- und hypervolemia and acute pulmonary hypertension as well as the influence of respiration and electrodes location. Correct quantification of cardiac output was obtained only in normovolemic circulation during exspiration (r = 0,96, m = 1,15, b = 0,04)."}
{"id": "PMID:1086008", "title": "[Cineradiographical studies of local ventricular motility before and after aorto-coronary bypass (author's transl)].", "content": "The purpose of this study was to observe the effect of surgical revascularization on the motility of the left ventricular segment distal to a high grade coronary stenosis. In 17 patients with LAD bypass local relative segment shortening lambda (lambda = diastolic length - systolic length/diastolic length X 100) was determined measuring the distances of coronary bifurcations and surgically implanted myocardial metal markers frame by frame in high speed cineradiography. A group of 14 patients whose angina pectoris was abolished after operation and where bypass proved to be patent showed significant increase of lambda within the first postoperative days (lambda preop. = 3,4 +/- 18,3 SD, lambda postop. = 18,9 +/- 8,0 SD, p less than 0.05). Paradoxical movement had disappeared in 4 cases. In the following two-monthly controls up to one year no significant further alterations of local motility were seen. Three patients with persisting angina did not show any increase of segment shortening. It is concluded that aorto-coronary bypass surgery can restore impaired local motility in the majority of patients. The method described is useful for repeated non-invasive controls of the success of operation.", "contents": "[Cineradiographical studies of local ventricular motility before and after aorto-coronary bypass (author's transl)]. The purpose of this study was to observe the effect of surgical revascularization on the motility of the left ventricular segment distal to a high grade coronary stenosis. In 17 patients with LAD bypass local relative segment shortening lambda (lambda = diastolic length - systolic length/diastolic length X 100) was determined measuring the distances of coronary bifurcations and surgically implanted myocardial metal markers frame by frame in high speed cineradiography. A group of 14 patients whose angina pectoris was abolished after operation and where bypass proved to be patent showed significant increase of lambda within the first postoperative days (lambda preop. = 3,4 +/- 18,3 SD, lambda postop. = 18,9 +/- 8,0 SD, p less than 0.05). Paradoxical movement had disappeared in 4 cases. In the following two-monthly controls up to one year no significant further alterations of local motility were seen. Three patients with persisting angina did not show any increase of segment shortening. It is concluded that aorto-coronary bypass surgery can restore impaired local motility in the majority of patients. The method described is useful for repeated non-invasive controls of the success of operation."}
{"id": "PMID:1086009", "title": "[Direct measurement of papillary muscle dynamics in the intact canine left ventricle during acute coronary occlusion (author's transl)].", "content": "The functions of the normal and ischemic papillary muscle in the intact canine heart were studied using an ultrasonic transit time method. Miniaturized piezoelectric crystals (1.5-2 mm 0) were implanted during extracorporeal circulation into the root and tip of the anterior and posterior papillary muscle of the left ventricle. 1. Phasic changes of left ventricular geometry during the heart cycle determine the dynamic pattern of papillary muscle movements. 2. The amplitude and velocity of papillary muscle length is directly related to those of myocardial wall segments. 3. Experimental ischemia induces total dysfunction of the papillary muscle within seconds. 4. Despite total muscle dysfunction no mitral insufficiency occured.", "contents": "[Direct measurement of papillary muscle dynamics in the intact canine left ventricle during acute coronary occlusion (author's transl)]. The functions of the normal and ischemic papillary muscle in the intact canine heart were studied using an ultrasonic transit time method. Miniaturized piezoelectric crystals (1.5-2 mm 0) were implanted during extracorporeal circulation into the root and tip of the anterior and posterior papillary muscle of the left ventricle. 1. Phasic changes of left ventricular geometry during the heart cycle determine the dynamic pattern of papillary muscle movements. 2. The amplitude and velocity of papillary muscle length is directly related to those of myocardial wall segments. 3. Experimental ischemia induces total dysfunction of the papillary muscle within seconds. 4. Despite total muscle dysfunction no mitral insufficiency occured."}
{"id": "PMID:1086010", "title": "[Effects of short time extreme hemodilution with and without hypothermia on hemodynamic and biochemical parameters in the pig (author's transl)].", "content": "Extreme hemodilution as \"Total Body Washout\" (TBW) is a feasible method for the clinical therapy of endo- or exogenous intoxication. This method was modified for a simple and quick setup. Landrace pigs were hemodiluted with a special heart-lung-machine. Within 4 minutes the hematocrit was lowered to less than 1%. The rectal temperature fell hereby in a normothermic group (n = 15) to 35, 1 +/- 1, 1 degrees C and in a hypothermic one (n = 13) to 31,1 +/- 1,3 degrees C. The TBW was followed by a total exchange transfusion. Most of the parameters that were studied did not show significant differences between cold and warm perfusion. The proceeding was well tolerated by the animals, they were observed up to 6 weeks. Disadvantages of initial surface cooling may be avoided by extreme hemodilution with exclusive extracorporal cooling, when cardiac arrest in hypothermia is intended.", "contents": "[Effects of short time extreme hemodilution with and without hypothermia on hemodynamic and biochemical parameters in the pig (author's transl)]. Extreme hemodilution as \"Total Body Washout\" (TBW) is a feasible method for the clinical therapy of endo- or exogenous intoxication. This method was modified for a simple and quick setup. Landrace pigs were hemodiluted with a special heart-lung-machine. Within 4 minutes the hematocrit was lowered to less than 1%. The rectal temperature fell hereby in a normothermic group (n = 15) to 35, 1 +/- 1, 1 degrees C and in a hypothermic one (n = 13) to 31,1 +/- 1,3 degrees C. The TBW was followed by a total exchange transfusion. Most of the parameters that were studied did not show significant differences between cold and warm perfusion. The proceeding was well tolerated by the animals, they were observed up to 6 weeks. Disadvantages of initial surface cooling may be avoided by extreme hemodilution with exclusive extracorporal cooling, when cardiac arrest in hypothermia is intended."}
{"id": "PMID:1086011", "title": "[Modern trends in the arteriography of the lower leg arteries (author's transl)].", "content": "The anatomical situation in arteries of the lower leg and foot can be demonstrated in an optimal manner with orthograde catheter-technique, biplane seriography, magnification and the use of the Medichrome-film. Angiography would be carried out in local anaesthesia after injection from Priscolin and an oral trunk of alcohol. This technique in 580 legs had no complications. All arteries behind arterial occlusions can be demonstrated before and after vascular surgery and other therapeutic procedures.", "contents": "[Modern trends in the arteriography of the lower leg arteries (author's transl)]. The anatomical situation in arteries of the lower leg and foot can be demonstrated in an optimal manner with orthograde catheter-technique, biplane seriography, magnification and the use of the Medichrome-film. Angiography would be carried out in local anaesthesia after injection from Priscolin and an oral trunk of alcohol. This technique in 580 legs had no complications. All arteries behind arterial occlusions can be demonstrated before and after vascular surgery and other therapeutic procedures."}
{"id": "PMID:1086012", "title": "[Results of reconstructive procedures concerning occlusive arterial disease of the full length of the trifurcation of the lower limb (author's transl)].", "content": "42 vascular reconstructive procedures, concerning occlusions in full length of the trifurcation of the lower limb, were carried out in 40 patients. The success-rate was unsatisfactory. It was as low as 55% after 30 days and 25% after 28 months. This is thought to be due to wrong indication and unfavorable operative proceedings. The high hospital letality of 12%, observed in aged persons only, may be reduced by improving post-operative care. It is stressed that any bad result of this kind of vascular surgery is connected with a high vital danger to the patient in question. Avoiding pre- and intraoperative unfavorable factors is rewarded by better early result; thus it is not the method itself but its wrong application which is bad.", "contents": "[Results of reconstructive procedures concerning occlusive arterial disease of the full length of the trifurcation of the lower limb (author's transl)]. 42 vascular reconstructive procedures, concerning occlusions in full length of the trifurcation of the lower limb, were carried out in 40 patients. The success-rate was unsatisfactory. It was as low as 55% after 30 days and 25% after 28 months. This is thought to be due to wrong indication and unfavorable operative proceedings. The high hospital letality of 12%, observed in aged persons only, may be reduced by improving post-operative care. It is stressed that any bad result of this kind of vascular surgery is connected with a high vital danger to the patient in question. Avoiding pre- and intraoperative unfavorable factors is rewarded by better early result; thus it is not the method itself but its wrong application which is bad."}
{"id": "PMID:1086013", "title": "[Complications of arterial reconstructions below the knee for chronic vascular occlusion (author's transl)].", "content": "From 1967 to 1976 49 reconstructions were undertaken in the area distal of the art. poplitea: 30 femoral-crural bypasses, 12 TEA und 7 orthogradic desobliterations. Complications until clinical demission were reviewed. There were in 8 cases postop. thrombosis which led to 4 amputations. 6 patients suffered from wound infection with one consecutive amputation. 4 hemmorages had to be stopped surgically. THE OVER ALL RATE OF COMPLICATIONS WAS 36,7% WITH A PRIMARY MORTALity of 4%.", "contents": "[Complications of arterial reconstructions below the knee for chronic vascular occlusion (author's transl)]. From 1967 to 1976 49 reconstructions were undertaken in the area distal of the art. poplitea: 30 femoral-crural bypasses, 12 TEA und 7 orthogradic desobliterations. Complications until clinical demission were reviewed. There were in 8 cases postop. thrombosis which led to 4 amputations. 6 patients suffered from wound infection with one consecutive amputation. 4 hemmorages had to be stopped surgically. THE OVER ALL RATE OF COMPLICATIONS WAS 36,7% WITH A PRIMARY MORTALity of 4%."}
{"id": "PMID:1086014", "title": "[Objective results of lumbar sympathectomy in peripheral arterial occlusive diseases (author's transl)].", "content": "A lumbar sympathectomy was performed on 36 patients with angiographical verified arterial occlusion on the upper and lower leg. Pre- and postoperative partly the lower leg flow was registered by air plethysmography and the lower leg and bridge of foot flow simultaneously registered by the Straine gauge plethysmography. Independent of the method an increase of the arterial blood flow was noticed with 40% of the patients. In spite of this improvement the registered values after the reactive hyperemia in lower leg and bridge of foot are still clearly restricted. The remaining 60% of the patients however showed a clear worthening of the blood flow conditions.", "contents": "[Objective results of lumbar sympathectomy in peripheral arterial occlusive diseases (author's transl)]. A lumbar sympathectomy was performed on 36 patients with angiographical verified arterial occlusion on the upper and lower leg. Pre- and postoperative partly the lower leg flow was registered by air plethysmography and the lower leg and bridge of foot flow simultaneously registered by the Straine gauge plethysmography. Independent of the method an increase of the arterial blood flow was noticed with 40% of the patients. In spite of this improvement the registered values after the reactive hyperemia in lower leg and bridge of foot are still clearly restricted. The remaining 60% of the patients however showed a clear worthening of the blood flow conditions."}
{"id": "PMID:1086015", "title": "[The prognosis in, and the limitations set to concervative treatment of occlusion of crural arteries (author's transl)].", "content": "Arterial occlusions limited to the lower leg generally respond well to therapeutic measures. Claudicatio intermittens can, in most instances, be adequately improved by exercise. Major amputations at stages III/IV can be avoided in practically all cases by conservative measures, provided the poplitea pulse is distinctly palpable. Patients with crural occlusion and necrosis tend to develop trophic lesions also at later times. Sympathectomy will be unable to prevent such recurrences where nicotine inhalation, the most important factor determining the prognosis, is continued.", "contents": "[The prognosis in, and the limitations set to concervative treatment of occlusion of crural arteries (author's transl)]. Arterial occlusions limited to the lower leg generally respond well to therapeutic measures. Claudicatio intermittens can, in most instances, be adequately improved by exercise. Major amputations at stages III/IV can be avoided in practically all cases by conservative measures, provided the poplitea pulse is distinctly palpable. Patients with crural occlusion and necrosis tend to develop trophic lesions also at later times. Sympathectomy will be unable to prevent such recurrences where nicotine inhalation, the most important factor determining the prognosis, is continued."}
{"id": "PMID:1086016", "title": "Protection against murine ascites tumours by lymphoid cell populations with T memory or cytotoxicity.", "content": "It was possible to protect irradiated C3H mice against lethal doses of allogeneic ascitic tumor cells (RBL-3 or P-815 Y) by systemic administration of low doses of syngeneic sensitized lymphoid cells. Two types of cell populations were active at similar doses: (1) spleen cells harvested 2 months after a nonlethal inoculation of tumour cells, at which time no cytotoxic lymphocytes wer in vivo sensitization or in vitro sensitized spleen cells; sucll and dose dependent and target cell specific. The findings imply that T memory cells as well as cytotoxic cells are able to protect, although it was not possible to exclude the presence of memory lymphocytes in the cytotoxic cell population. Antiserum to cytotoxic T cells (CTL) was prepared in an attempt to distinguish memory and cytotoxic effector cells. The antiserum did not react with the majority of T cells in a normal spleen or thymus, but showed specificity for the CTL cell lineage. Antiserum treatment and complement abolished protection in all types of sensitized cell populations. Since memory and cytotoxic cells appeared to share differentiation antigens, we could not establish whether memory cells are precursors or products of cytotoxic cells.", "contents": "Protection against murine ascites tumours by lymphoid cell populations with T memory or cytotoxicity. It was possible to protect irradiated C3H mice against lethal doses of allogeneic ascitic tumor cells (RBL-3 or P-815 Y) by systemic administration of low doses of syngeneic sensitized lymphoid cells. Two types of cell populations were active at similar doses: (1) spleen cells harvested 2 months after a nonlethal inoculation of tumour cells, at which time no cytotoxic lymphocytes wer in vivo sensitization or in vitro sensitized spleen cells; sucll and dose dependent and target cell specific. The findings imply that T memory cells as well as cytotoxic cells are able to protect, although it was not possible to exclude the presence of memory lymphocytes in the cytotoxic cell population. Antiserum to cytotoxic T cells (CTL) was prepared in an attempt to distinguish memory and cytotoxic effector cells. The antiserum did not react with the majority of T cells in a normal spleen or thymus, but showed specificity for the CTL cell lineage. Antiserum treatment and complement abolished protection in all types of sensitized cell populations. Since memory and cytotoxic cells appeared to share differentiation antigens, we could not establish whether memory cells are precursors or products of cytotoxic cells."}
{"id": "PMID:1086021", "title": "[Perforation of gastric cancer].", "content": "An analysis of treatment of 85 patients with perforating cancerous tumor of the stomach is presented. Males--63, females--22. The patients' age ranged from 32 to 85 years. Sixty seven patients were operatd upon, 35 of them died. Eighteen patients were not subjected to surgery, all of them died. The following variants of complications associated with gastric cancer are noted: cancer perforation--in 32, hemorrhage and the perforation--in 35, perforation and hemorrhage occurred simultaneously--in 15, perforation followed by hemorrhage--in 3. High mortality rate was due to grave patients' condition, difficulties and errors of diagnosis, inadequacy of therapeutic measures, late operative interventions. The results obtained are described.", "contents": "[Perforation of gastric cancer]. An analysis of treatment of 85 patients with perforating cancerous tumor of the stomach is presented. Males--63, females--22. The patients' age ranged from 32 to 85 years. Sixty seven patients were operatd upon, 35 of them died. Eighteen patients were not subjected to surgery, all of them died. The following variants of complications associated with gastric cancer are noted: cancer perforation--in 32, hemorrhage and the perforation--in 35, perforation and hemorrhage occurred simultaneously--in 15, perforation followed by hemorrhage--in 3. High mortality rate was due to grave patients' condition, difficulties and errors of diagnosis, inadequacy of therapeutic measures, late operative interventions. The results obtained are described."}
{"id": "PMID:1086022", "title": "[Complications of benign nonepithelial tumors of the gastrointestinal tract].", "content": "The results of observations over 8 patients with complicated forms of benign nonepithelial tumors of abdominal organs are reported, including leiomyoma--in 7 cases and neurinoma--in 1. Isocomplications of this group of tumors in 5 patients were as follows: invagination of the small intestin--in 1, perforation and malignant transformation of the tumor--in 1. The patients were treated surgically. Resection of the organ involved seems to be preferable.", "contents": "[Complications of benign nonepithelial tumors of the gastrointestinal tract]. The results of observations over 8 patients with complicated forms of benign nonepithelial tumors of abdominal organs are reported, including leiomyoma--in 7 cases and neurinoma--in 1. Isocomplications of this group of tumors in 5 patients were as follows: invagination of the small intestin--in 1, perforation and malignant transformation of the tumor--in 1. The patients were treated surgically. Resection of the organ involved seems to be preferable."}
{"id": "PMID:1086035", "title": "[Quantitative determination of Willebrand factor activity in Willebrand's disease].", "content": "Certain new data about the nature of Willebrand factors are discussed, its activity being considered as part of the activity of the antihemolytic factor VIII. An one-stage method for its determination is described based on the absence of thrombocytic aggregation in the presence of ristocetin in the patients with Willebrand disease as well as the illustration of one case.", "contents": "[Quantitative determination of Willebrand factor activity in Willebrand's disease]. Certain new data about the nature of Willebrand factors are discussed, its activity being considered as part of the activity of the antihemolytic factor VIII. An one-stage method for its determination is described based on the absence of thrombocytic aggregation in the presence of ristocetin in the patients with Willebrand disease as well as the illustration of one case."}
{"id": "PMID:1086037", "title": "Outpatient heroin detoxification with acupuncture and staplepuncture.", "content": "Eighteen heroin addicts were treated as outpatients with acupuncture, electrical stimulation and staplepuncture. Results of treatment were compared with results in two similar groups of 18 persons in whom detoxification was carried out using methadone and propoxyphene napsylate. Withdrawal symptoms were relieved for about two hours in most of the patients after a treatment episode of acupuncture and electrical stimulation. Staplepuncture, which is the manipulation by hand of a surgical staple implanted in the concha of the ear, was reported to relieve withdrawal symptoms at least partially in approximately 40 percent of subjects. In only one person of the group treated with acupuncture or staplepuncture was complete detoxification achieved, compared with 13 and 10 persons, respectively, in the methadone and propoxyphene napsylate groups (p<.001). Use of acupuncture and staplepuncture in outpatient clinics may be limited unless techniques can be found that will relieve withdrawal symptoms for a longer period than that observed in this study.", "contents": "Outpatient heroin detoxification with acupuncture and staplepuncture. Eighteen heroin addicts were treated as outpatients with acupuncture, electrical stimulation and staplepuncture. Results of treatment were compared with results in two similar groups of 18 persons in whom detoxification was carried out using methadone and propoxyphene napsylate. Withdrawal symptoms were relieved for about two hours in most of the patients after a treatment episode of acupuncture and electrical stimulation. Staplepuncture, which is the manipulation by hand of a surgical staple implanted in the concha of the ear, was reported to relieve withdrawal symptoms at least partially in approximately 40 percent of subjects. In only one person of the group treated with acupuncture or staplepuncture was complete detoxification achieved, compared with 13 and 10 persons, respectively, in the methadone and propoxyphene napsylate groups (p<.001). Use of acupuncture and staplepuncture in outpatient clinics may be limited unless techniques can be found that will relieve withdrawal symptoms for a longer period than that observed in this study."}
{"id": "PMID:1086038", "title": "[Diagnosis, therapy and sequelae of upper gastrointestinal haemorrhage in acute pancreatis].", "content": "Gastrointestinal bleeding complicating pancreatitis is a grave development which carries a high mortality rate. Ten out of 450 patients treated by us for pancreatitis developed gastrointestinal haemorrhage; 7 of these patients died, 6 of whom had a haemorrhagic necrotic, and one a so-called oedematous form of pancreatitis as diagnosed at autopsy. Nine operations were performed, most of them being only drainage procedures. Persistent shock or recurrence of severe symptoms with constant chemical pathological changes in the course of acute pancreatitis call for the rapid initiation of an aggressive approach towards diagnosis (endoscopy, angiography) and therapy, with radical surgical procedures, as indicated, aimed at rectifying the extensive pathology. We believe that these are the only possible means of achieving a reduction in the extremely high mortality rate in these cases and of avoiding late complications such as pseudocysts with the inherent danger of bleeding, abscesses and portal hypertension.", "contents": "[Diagnosis, therapy and sequelae of upper gastrointestinal haemorrhage in acute pancreatis]. Gastrointestinal bleeding complicating pancreatitis is a grave development which carries a high mortality rate. Ten out of 450 patients treated by us for pancreatitis developed gastrointestinal haemorrhage; 7 of these patients died, 6 of whom had a haemorrhagic necrotic, and one a so-called oedematous form of pancreatitis as diagnosed at autopsy. Nine operations were performed, most of them being only drainage procedures. Persistent shock or recurrence of severe symptoms with constant chemical pathological changes in the course of acute pancreatitis call for the rapid initiation of an aggressive approach towards diagnosis (endoscopy, angiography) and therapy, with radical surgical procedures, as indicated, aimed at rectifying the extensive pathology. We believe that these are the only possible means of achieving a reduction in the extremely high mortality rate in these cases and of avoiding late complications such as pseudocysts with the inherent danger of bleeding, abscesses and portal hypertension."}
{"id": "PMID:1086042", "title": "[Chronic lymphadenosis--short review].", "content": "It was tried to show in form of a survey some molecular-biological, immunopathological and kinetic aspects of the CL (chronic lymphadenosis)-lymphocytes, which seem to be significant for the pathogenesis of chronic lymphadenosis. Apart from this, the modern therapeutic possibilities concerning the inhibition of proliferation as well as the depletion which seem to be suitable for influencing the still fatal course of chronic lymphadenosis are discussed.", "contents": "[Chronic lymphadenosis--short review]. It was tried to show in form of a survey some molecular-biological, immunopathological and kinetic aspects of the CL (chronic lymphadenosis)-lymphocytes, which seem to be significant for the pathogenesis of chronic lymphadenosis. Apart from this, the modern therapeutic possibilities concerning the inhibition of proliferation as well as the depletion which seem to be suitable for influencing the still fatal course of chronic lymphadenosis are discussed."}
{"id": "PMID:1086043", "title": "[Effect of phenylalanine-vasopressin on the lower esophageal sphincter. Possible implications in the treatment of bleeding esophageal varices].", "content": "Vasopressin and its analogs are used inthe treatment of bleeding esophageal varices. Since gastrointestinal reflux may have a deleterious effect on variceal hemorrhage, the effect of 2,3-phenylalanine-8-lysine-vasopressin upon the lower esophageal sphincter (LES) was studies by rapid pull-through manometry in 24 persons. PLV infusion up to a dosis of 2.7 mU/kg/h raised LES pressure from 15.1 +/- 1.3 (SEM) to 17.9 +/- 2.0 mm Hg. Higher doses lowered LES pressure progressively to 12.1 +/- 0.7 mmHg at 54 mU/kg/h. The serum gastrin level did neither correlate with basal LES pressure not with LES pressure changes during PLV infusion. Therefore, PLV does not appear to act indirectly through serum gastrin. Because of the danger of systemic side effects and of the undesirable in LES pressure with the usual high doses of vasoactive substances, a continuous infusion of lower doses of vasopressin analogs appears to be advantageous.", "contents": "[Effect of phenylalanine-vasopressin on the lower esophageal sphincter. Possible implications in the treatment of bleeding esophageal varices]. Vasopressin and its analogs are used inthe treatment of bleeding esophageal varices. Since gastrointestinal reflux may have a deleterious effect on variceal hemorrhage, the effect of 2,3-phenylalanine-8-lysine-vasopressin upon the lower esophageal sphincter (LES) was studies by rapid pull-through manometry in 24 persons. PLV infusion up to a dosis of 2.7 mU/kg/h raised LES pressure from 15.1 +/- 1.3 (SEM) to 17.9 +/- 2.0 mm Hg. Higher doses lowered LES pressure progressively to 12.1 +/- 0.7 mmHg at 54 mU/kg/h. The serum gastrin level did neither correlate with basal LES pressure not with LES pressure changes during PLV infusion. Therefore, PLV does not appear to act indirectly through serum gastrin. Because of the danger of systemic side effects and of the undesirable in LES pressure with the usual high doses of vasoactive substances, a continuous infusion of lower doses of vasopressin analogs appears to be advantageous."}
{"id": "PMID:1086044", "title": "[Endoscopic diagnosis of carcinoma in the operated stomach].", "content": "In 24 000 gastroscopies at 4 medical departments 705 subjects with gastric operations done more than 5 years before had been studied. 39 of 608 patients with Billroth II, 5 of 81 with Billroth I and 4 out of 15 with gastroenterostomy were found to be suffering from carcinoma of the gastric stump. The primary gastric operations were performed at a medium age of 40 years. The interval between surgery and development of carcinoma averaged 24 years and was shorter in patients operated at a higher age. Besides age and interval since operation risk factors were apparently former gastric ulcers or polyps. Indications for endoscopic follow-up studies of gastrectomized patients to improve surgical results with carcinoma of the gastric stump have been suggested.", "contents": "[Endoscopic diagnosis of carcinoma in the operated stomach]. In 24 000 gastroscopies at 4 medical departments 705 subjects with gastric operations done more than 5 years before had been studied. 39 of 608 patients with Billroth II, 5 of 81 with Billroth I and 4 out of 15 with gastroenterostomy were found to be suffering from carcinoma of the gastric stump. The primary gastric operations were performed at a medium age of 40 years. The interval between surgery and development of carcinoma averaged 24 years and was shorter in patients operated at a higher age. Besides age and interval since operation risk factors were apparently former gastric ulcers or polyps. Indications for endoscopic follow-up studies of gastrectomized patients to improve surgical results with carcinoma of the gastric stump have been suggested."}
{"id": "PMID:1086045", "title": "[Autoimmune hemolytic anemia with Donath-Landsteiner antibodies and antinuclear factors during the course of chronic-aggressive hepatitis].", "content": "With the onset of a remission phase of chronic aggressive hepatitis in a young female patient, two different auto-antibodies were found which had not previously been in evidence. These antibodies were identified as Donath-Landsteiner antibodies, the cause of a secondary auto-immune hemolytic anemia observed during this period, and anti-nuclear antibodies. Both antibodies were of the IgG class, both could be adsorbed onto the patient's own erythrocytes as well as P1-positive test erythrocytes, and both antibodies were present in the subsequent erythrocyte eluate. Separation of the two antibodies present in the serum as well as in the antibody-containing erythrocyte eluate was possible by absorption of the antinuclear factors using preparations of liver and thymus cell nuclei, but the anti-erythrocyte activity was not diminished by this procedure. Rapid remission of the auto-immune hemolytic anemia was achieved by a therapeutic application of corticosteroids. Long-term therapy has resulted in no recurrence of the auto-immune hemolytic anemia until now.", "contents": "[Autoimmune hemolytic anemia with Donath-Landsteiner antibodies and antinuclear factors during the course of chronic-aggressive hepatitis]. With the onset of a remission phase of chronic aggressive hepatitis in a young female patient, two different auto-antibodies were found which had not previously been in evidence. These antibodies were identified as Donath-Landsteiner antibodies, the cause of a secondary auto-immune hemolytic anemia observed during this period, and anti-nuclear antibodies. Both antibodies were of the IgG class, both could be adsorbed onto the patient's own erythrocytes as well as P1-positive test erythrocytes, and both antibodies were present in the subsequent erythrocyte eluate. Separation of the two antibodies present in the serum as well as in the antibody-containing erythrocyte eluate was possible by absorption of the antinuclear factors using preparations of liver and thymus cell nuclei, but the anti-erythrocyte activity was not diminished by this procedure. Rapid remission of the auto-immune hemolytic anemia was achieved by a therapeutic application of corticosteroids. Long-term therapy has resulted in no recurrence of the auto-immune hemolytic anemia until now."}
{"id": "PMID:1086046", "title": "T lymphocytes in chronic lymphocytic leukemia.", "content": "In cultures of lymphocytes from the peripheral blood of patients with chronic lymphocytic leukemia, the approximate magnitude of the population of lymphocytes characterized by delayed PHA reactivity was estimated. To this end, cells from cultures in an early stage of development were pulse-labeled with 3H-TdR, and their multiplication was expressed by an index of proliferation. On the basis of these data, the proportion of unlabeled blasts in the late stage of cultures was calculated in relation to the late-reacting raction to the mitogen. This fraction comprised 50-100% of blasts in late cultures (6 days). The subpopulation was not detected in only 2 of 17 cases. Its magnitude in the peripheral blood amounted to about 3% of the lymphocytes and nearly one half of the total number of lymphocytes reacting to PHA in patients with CLL. It was concluded that the number of peripheral blood lymphocytes reacting to PHA is not diminished at all in patients with leukocytosis between 11,000 and 17,000/mul. The late-reactive population was relatively resistant to X radiation, dependent on L-asparagine, and insensitive to doses of colchicine which are toxic for leukemic cells. The ability of these lymphocytes to form rosettes spontaneously with sheep red blood cells is preserved in a large measure. Only a small proportion lacked this surface marker. It seems that the population of late-reacting lymphocytes to PHA in the blood of patients with CLL is of thymic origin.", "contents": "T lymphocytes in chronic lymphocytic leukemia. In cultures of lymphocytes from the peripheral blood of patients with chronic lymphocytic leukemia, the approximate magnitude of the population of lymphocytes characterized by delayed PHA reactivity was estimated. To this end, cells from cultures in an early stage of development were pulse-labeled with 3H-TdR, and their multiplication was expressed by an index of proliferation. On the basis of these data, the proportion of unlabeled blasts in the late stage of cultures was calculated in relation to the late-reacting raction to the mitogen. This fraction comprised 50-100% of blasts in late cultures (6 days). The subpopulation was not detected in only 2 of 17 cases. Its magnitude in the peripheral blood amounted to about 3% of the lymphocytes and nearly one half of the total number of lymphocytes reacting to PHA in patients with CLL. It was concluded that the number of peripheral blood lymphocytes reacting to PHA is not diminished at all in patients with leukocytosis between 11,000 and 17,000/mul. The late-reactive population was relatively resistant to X radiation, dependent on L-asparagine, and insensitive to doses of colchicine which are toxic for leukemic cells. The ability of these lymphocytes to form rosettes spontaneously with sheep red blood cells is preserved in a large measure. Only a small proportion lacked this surface marker. It seems that the population of late-reacting lymphocytes to PHA in the blood of patients with CLL is of thymic origin."}
{"id": "PMID:1086047", "title": "[Absecence of the antidepressant action of lithium under clinical and experimental conditions].", "content": "During a long-term treatment by lihtium carbonate of 200 patients with manic-depressive psychosis the authors did not find any antidepressive effect. In some cases administration of lithium during the depressive phases aggravated the depressive symptomatology, prolonged the duration of the phase, increased the therapeutical resistance. In experiments with lithium on animals there were no typical traits for an antidepressive effect: adrenopositive and serotoninopositive action.", "contents": "[Absecence of the antidepressant action of lithium under clinical and experimental conditions]. During a long-term treatment by lihtium carbonate of 200 patients with manic-depressive psychosis the authors did not find any antidepressive effect. In some cases administration of lithium during the depressive phases aggravated the depressive symptomatology, prolonged the duration of the phase, increased the therapeutical resistance. In experiments with lithium on animals there were no typical traits for an antidepressive effect: adrenopositive and serotoninopositive action."}
{"id": "PMID:1086049", "title": "Effects of lymphocytotoxins of systemic lupus erythematosus (SLE) sera on T and B lymphocytes.", "content": "SLE lymphocytotoxins can be demonstrated on the lymphocytes by immunofluorescence. Complement addition lyses the cells covered with LCT. The proportion of B and T cells as determined by surface immunoglobulins and spontaneous rosette formation of lymphocytes from healthy donors remains unchanged after LCT and complement treatment. The proportion of B and T cells of SLE lymphocytes was altered after cell lysis. The B cells seemed to be relatively resistant, and the T cells more susceptible, to autologous LCT. Thus the relative decrease in the number of T cells in active SLE may be the consequence of surface bound LCT with its effect on the circulating T cells.", "contents": "Effects of lymphocytotoxins of systemic lupus erythematosus (SLE) sera on T and B lymphocytes. SLE lymphocytotoxins can be demonstrated on the lymphocytes by immunofluorescence. Complement addition lyses the cells covered with LCT. The proportion of B and T cells as determined by surface immunoglobulins and spontaneous rosette formation of lymphocytes from healthy donors remains unchanged after LCT and complement treatment. The proportion of B and T cells of SLE lymphocytes was altered after cell lysis. The B cells seemed to be relatively resistant, and the T cells more susceptible, to autologous LCT. Thus the relative decrease in the number of T cells in active SLE may be the consequence of surface bound LCT with its effect on the circulating T cells."}
{"id": "PMID:1086050", "title": "Assay of factors of the kinin system in plasma from patients with specific exogenous allergies.", "content": "Factors of significance for the release of kinin were estimated in plasma from patients with specific, exogenous allergies and in that from healthy individuals. There was no evidence that the rapid initial kinin release in plasma from allergic patients caused by submaximum concentrations of hog pancreas kalikrein or by acetone-activated human plasma (2) was due to an increased level of prekallikrein activator (activated factor XII), to prekallikrein itself or to a factor possibly positioned between active factor XII and prekallikrein. In addition, the rapid halt in kinin release observed for the patient plasma could not be ascribed to an increased level of alpha2-macroglobulin, CI-inactivator or alpha1-antitrypsin. It is suggested that the differences in kinin release registered between the patient plasma and the normal plasma might reflect differences in the relative amounts of kininogen fractions present.", "contents": "Assay of factors of the kinin system in plasma from patients with specific exogenous allergies. Factors of significance for the release of kinin were estimated in plasma from patients with specific, exogenous allergies and in that from healthy individuals. There was no evidence that the rapid initial kinin release in plasma from allergic patients caused by submaximum concentrations of hog pancreas kalikrein or by acetone-activated human plasma (2) was due to an increased level of prekallikrein activator (activated factor XII), to prekallikrein itself or to a factor possibly positioned between active factor XII and prekallikrein. In addition, the rapid halt in kinin release observed for the patient plasma could not be ascribed to an increased level of alpha2-macroglobulin, CI-inactivator or alpha1-antitrypsin. It is suggested that the differences in kinin release registered between the patient plasma and the normal plasma might reflect differences in the relative amounts of kininogen fractions present."}
{"id": "PMID:1086051", "title": "The dependence of tension relaxation in skeletal muscle on the number of sarcomeres in series.", "content": "Mulieri (1972) has reported that the depth of the latency relaxation, tension relaxation, changes only insignificantly when about half of a fibre bundle is wound tightly around a small glass rod. From this observation he concludes that the tension relaxation is not correlated with the number of sarcomeres in series in a frog muscle fibre. The dependency of the tension relaxation on the number of sarcomeres in series has been reinvestigated using both Mulieri's method and other independent methods. When Mulieri's experimental procedure was repeated, his result was reproduced. In the other methods used the tension relaxation was found to be proportional to the number of sarcomeres in series which were activated initially. This result was also obtained by introducing a slight modification to the procedure used by Mulieri. The results could equally well be understood if the latency relaxation was caused by a definite elongation rather than an increase in compliance of the fibre.", "contents": "The dependence of tension relaxation in skeletal muscle on the number of sarcomeres in series. Mulieri (1972) has reported that the depth of the latency relaxation, tension relaxation, changes only insignificantly when about half of a fibre bundle is wound tightly around a small glass rod. From this observation he concludes that the tension relaxation is not correlated with the number of sarcomeres in series in a frog muscle fibre. The dependency of the tension relaxation on the number of sarcomeres in series has been reinvestigated using both Mulieri's method and other independent methods. When Mulieri's experimental procedure was repeated, his result was reproduced. In the other methods used the tension relaxation was found to be proportional to the number of sarcomeres in series which were activated initially. This result was also obtained by introducing a slight modification to the procedure used by Mulieri. The results could equally well be understood if the latency relaxation was caused by a definite elongation rather than an increase in compliance of the fibre."}
{"id": "PMID:1086053", "title": "An immunological approach to dementia in the elderly.", "content": "A study into the relationship of immune change in the serum and the presence of senile dementia is reported. Three groups were studied, senile dementia, cerebrovascular disease and subjects without evidence of brain disease. All were aged 65 years and over. The immunofluorescent studies showed an excess of antineuronal reactivity and a fall in antinuclear antibody in females with senile dementia. There was no significant difference between the groups in respect of immunoglobulins, slide latex and a complement fixation test, against a variety of tissues. The significance of the findings in relation to other published results is discussed.", "contents": "An immunological approach to dementia in the elderly. A study into the relationship of immune change in the serum and the presence of senile dementia is reported. Three groups were studied, senile dementia, cerebrovascular disease and subjects without evidence of brain disease. All were aged 65 years and over. The immunofluorescent studies showed an excess of antineuronal reactivity and a fall in antinuclear antibody in females with senile dementia. There was no significant difference between the groups in respect of immunoglobulins, slide latex and a complement fixation test, against a variety of tissues. The significance of the findings in relation to other published results is discussed."}
{"id": "PMID:1086054", "title": "Presumptive relationships between ventricular proliferaion and development of the lateral motor columns in the spinal cord of Rana pipiens larvae.", "content": "The extent of mitotic activity in the proliferative ventricular zone of the developing frog (Rana pipiens) spinal cord is a function of both the longitudinal cord level and the developmental stage. Counts of mitotic cells in the ventricular zone demonstrated higher levels of proliferation in the dorsal than ventral halves of the spinal cord with decreasing total proliferative activity from the early to late larval (tadpole) stages. Mitoses were virtually absent from the ventricular zone by the conclusion of metamorphosis. Changes in mitotic counts at different levels of the spinal cord can be correlated with the presence or absence of the brachial or lumbosacral pairs of lateral motor columns. A parallel also exists between the caudo-cephalic direction of motor column development and a similar progression of mitotic activity in the ventricular zone, a portion of which gives rise to the spinal motor neurons. It is suggested that proliferation in the ventricular zone during the larval frog stages contributes to the presumptive motor neuron population and migrates into the lateral motor columns during their later maturation.", "contents": "Presumptive relationships between ventricular proliferaion and development of the lateral motor columns in the spinal cord of Rana pipiens larvae. The extent of mitotic activity in the proliferative ventricular zone of the developing frog (Rana pipiens) spinal cord is a function of both the longitudinal cord level and the developmental stage. Counts of mitotic cells in the ventricular zone demonstrated higher levels of proliferation in the dorsal than ventral halves of the spinal cord with decreasing total proliferative activity from the early to late larval (tadpole) stages. Mitoses were virtually absent from the ventricular zone by the conclusion of metamorphosis. Changes in mitotic counts at different levels of the spinal cord can be correlated with the presence or absence of the brachial or lumbosacral pairs of lateral motor columns. A parallel also exists between the caudo-cephalic direction of motor column development and a similar progression of mitotic activity in the ventricular zone, a portion of which gives rise to the spinal motor neurons. It is suggested that proliferation in the ventricular zone during the larval frog stages contributes to the presumptive motor neuron population and migrates into the lateral motor columns during their later maturation."}
{"id": "PMID:1086055", "title": "Intraperitoneal and intragastric norepinephrine instillation in massive gastrointestinal bleeding--clinical study. Preliminary report.", "content": "Intraperitoneal and intragastric instillation of norepinephrine in high concentration can arrest bleeding of various origins in the gastrointestinal tract. Of the 21 cases studied, the hemorrhage was controlled in 80% of cases. This form of therapy is simple, prompt, safe and can be performed as a bed-side therapeutic procedure to stop life-threatening gastrointestinal tract bleeding. Intraperitoneal instillation of norepinephrine should not be used more than once, if the bleeding is not controlled. Intragastric norepinephrine instillations may be repeated any number of times if required.", "contents": "Intraperitoneal and intragastric norepinephrine instillation in massive gastrointestinal bleeding--clinical study. Preliminary report. Intraperitoneal and intragastric instillation of norepinephrine in high concentration can arrest bleeding of various origins in the gastrointestinal tract. Of the 21 cases studied, the hemorrhage was controlled in 80% of cases. This form of therapy is simple, prompt, safe and can be performed as a bed-side therapeutic procedure to stop life-threatening gastrointestinal tract bleeding. Intraperitoneal instillation of norepinephrine should not be used more than once, if the bleeding is not controlled. Intragastric norepinephrine instillations may be repeated any number of times if required."}
{"id": "PMID:1086057", "title": "Idiopathic portal hypertension.", "content": "Idiopathic portal hypertension is reported in five cases including one case of chronic arsenical intake and one case of chronic industrial vinyl chloride exposure. In all five cases the patients presented with gastrointestinal bleeding as the chief complaint. Physical examination was within normal limits except for splenomegaly in all. Results of liver function tests were normal, except for the relative clearance of sulfobromophtalein. A surgical liver biopsy specimen was obtained in all cases and showed moderate degrees of portal fibrosis, but no cirrhosis. Combined umbilicoportal, hepatic vein and superior mesenteric artery catheterization was performed in all cases. Hepatoportographies showed distortion of the intrahepatic portal venous system and cut-off of small portal venules. Porto-hepatic gradients ranged from 14.0 to 20.5 mm Hg. The portal hypertension was both sinusoidal and presinusoidal in nature but mainly presinusoidal. Hepatic extraction of indocyanine green and of albumin microaggregates was normal, thereby suggesting normal functional portal blood supply to the liver. The patients with arsenical or vinyl chloride exposure could not be differentiated from the other three patients with idiopathic portal hypertension. These results suggest that idiopathic portal hypertension may be related to domestic or industrial exposure to other hepatotoxins.", "contents": "Idiopathic portal hypertension. Idiopathic portal hypertension is reported in five cases including one case of chronic arsenical intake and one case of chronic industrial vinyl chloride exposure. In all five cases the patients presented with gastrointestinal bleeding as the chief complaint. Physical examination was within normal limits except for splenomegaly in all. Results of liver function tests were normal, except for the relative clearance of sulfobromophtalein. A surgical liver biopsy specimen was obtained in all cases and showed moderate degrees of portal fibrosis, but no cirrhosis. Combined umbilicoportal, hepatic vein and superior mesenteric artery catheterization was performed in all cases. Hepatoportographies showed distortion of the intrahepatic portal venous system and cut-off of small portal venules. Porto-hepatic gradients ranged from 14.0 to 20.5 mm Hg. The portal hypertension was both sinusoidal and presinusoidal in nature but mainly presinusoidal. Hepatic extraction of indocyanine green and of albumin microaggregates was normal, thereby suggesting normal functional portal blood supply to the liver. The patients with arsenical or vinyl chloride exposure could not be differentiated from the other three patients with idiopathic portal hypertension. These results suggest that idiopathic portal hypertension may be related to domestic or industrial exposure to other hepatotoxins."}
{"id": "PMID:1086058", "title": "Nephrotic syndrome in Hodgkin's disease. Evidence for pathogenesis alternative to immune complex deposition.", "content": "The nephrotic syndrome has been reported to occur in patients with Hodgkin's disease even in the absence of amyloidosis, tumor infiltration of renal vein thrombosis. Three patients are presented with Hodgkin's disease and the nephrotic syndrome whose renal biopsy specimens studied with light, immunofluorescence and electron microscopy were compatible with \"lipoid nephrosis\" (minimal change disease). A review of the literature reveals 35 patients with Hodgkin's disease and the nephrotic syndrome. Renal tissue was available for examination in only 27 patients. The majority of patients apparently had glomerular alterations consistent with lipoid nephrosis. The nephrotic syndrome in most of these patients remitted with a variety of methods of therapy (including excision, irradiation, prednisone and cyclophosphamide) and tended to relapse with a recurrence of Hodgkin's disease. In three-fourths of the patients with Hodgkin's disease and the nephrotic syndrome, the Hodgkin's disease was of a mixed cellularity type. The etiology of lipoid nephrosis, although unclear, may be a consequence of altered lymphocyte function. Hodgkin's disease is a malignancy involving T lymphocytes, and the nephrotic syndrome occurring in the course of Hodgkin's disease may be a  result of an adverse effect of glomeruli by products of tumor lymphocytes rather than of glomerular deposition of immune complexes.", "contents": "Nephrotic syndrome in Hodgkin's disease. Evidence for pathogenesis alternative to immune complex deposition. The nephrotic syndrome has been reported to occur in patients with Hodgkin's disease even in the absence of amyloidosis, tumor infiltration of renal vein thrombosis. Three patients are presented with Hodgkin's disease and the nephrotic syndrome whose renal biopsy specimens studied with light, immunofluorescence and electron microscopy were compatible with \"lipoid nephrosis\" (minimal change disease). A review of the literature reveals 35 patients with Hodgkin's disease and the nephrotic syndrome. Renal tissue was available for examination in only 27 patients. The majority of patients apparently had glomerular alterations consistent with lipoid nephrosis. The nephrotic syndrome in most of these patients remitted with a variety of methods of therapy (including excision, irradiation, prednisone and cyclophosphamide) and tended to relapse with a recurrence of Hodgkin's disease. In three-fourths of the patients with Hodgkin's disease and the nephrotic syndrome, the Hodgkin's disease was of a mixed cellularity type. The etiology of lipoid nephrosis, although unclear, may be a consequence of altered lymphocyte function. Hodgkin's disease is a malignancy involving T lymphocytes, and the nephrotic syndrome occurring in the course of Hodgkin's disease may be a  result of an adverse effect of glomeruli by products of tumor lymphocytes rather than of glomerular deposition of immune complexes."}
{"id": "PMID:1086060", "title": "Fundus flavimaculatus and Stargardt's disease.", "content": "Of 42 patients studied who had fundus flavimaculatus and Stargardt's disease, most had reduced visual acuity due to an atrophic macular lesion. Family histories were consistent with autosomal-recessive inheritance. In some young patients, the yellow-white flecks developed with time, while in some older patients the flecks faded, pari passau with increasing retinal pigment epithelial atrophy. Fluorescein angiography showed that most white flecks were largely or totally nonfluorescent. Even after the flecks had faded from view, the sites where they had been usually remained nonfluorescent. In patients with lesions confined to the posterior pole, the electroretinogram and electro-oculogram were usually normal; in patients with widespread lesions, one or both tests were usually abnormal. Affected members of any one family had a similar distribution of fundus lesions.", "contents": "Fundus flavimaculatus and Stargardt's disease. Of 42 patients studied who had fundus flavimaculatus and Stargardt's disease, most had reduced visual acuity due to an atrophic macular lesion. Family histories were consistent with autosomal-recessive inheritance. In some young patients, the yellow-white flecks developed with time, while in some older patients the flecks faded, pari passau with increasing retinal pigment epithelial atrophy. Fluorescein angiography showed that most white flecks were largely or totally nonfluorescent. Even after the flecks had faded from view, the sites where they had been usually remained nonfluorescent. In patients with lesions confined to the posterior pole, the electroretinogram and electro-oculogram were usually normal; in patients with widespread lesions, one or both tests were usually abnormal. Affected members of any one family had a similar distribution of fundus lesions."}
{"id": "PMID:1086061", "title": "Fluorescein angiography and vitamin A and oxalate levels in fundus albipunctatus.", "content": "Two patients had fundus albipunctatus, one of the variants of congenital stationary night blindness. Neither the white dots in the retinas of these patients nor the retarded course of dark adaptation characteristically associated with the disease could be attributed to vitamin A deficiency or raised oxalate levels since both substances were present in normal amounts. Fluorescin angiography showed multiple discrete lesions in the pigment epithelium not coincident with the ophthalmoscopically visible changes. Since the pigment epithelium is the storage site for bleached visual pigment, these findings suggest abnormalities that underlie the major functional deficit in this disease, that is, the slow recovery of retinal sensitivity.", "contents": "Fluorescein angiography and vitamin A and oxalate levels in fundus albipunctatus. Two patients had fundus albipunctatus, one of the variants of congenital stationary night blindness. Neither the white dots in the retinas of these patients nor the retarded course of dark adaptation characteristically associated with the disease could be attributed to vitamin A deficiency or raised oxalate levels since both substances were present in normal amounts. Fluorescin angiography showed multiple discrete lesions in the pigment epithelium not coincident with the ophthalmoscopically visible changes. Since the pigment epithelium is the storage site for bleached visual pigment, these findings suggest abnormalities that underlie the major functional deficit in this disease, that is, the slow recovery of retinal sensitivity."}
{"id": "PMID:1086062", "title": "Temporal artery biopsy in herpes zoster ophthalmicus with delayed arteritis.", "content": "A 58-year-old man developed herpes zoster ophthalmicus with delayed hemiparesis. Temporal artery biopsy confirmed the presence of a vasculitis. Electron microscopy of the temporal artery failed to reveal viral particles. Herpes zoster ophthalmicus with delayed arteritis appeared to be a contiguous spread of vasculitis to the carotid system and not a direct viral invasion.", "contents": "Temporal artery biopsy in herpes zoster ophthalmicus with delayed arteritis. A 58-year-old man developed herpes zoster ophthalmicus with delayed hemiparesis. Temporal artery biopsy confirmed the presence of a vasculitis. Electron microscopy of the temporal artery failed to reveal viral particles. Herpes zoster ophthalmicus with delayed arteritis appeared to be a contiguous spread of vasculitis to the carotid system and not a direct viral invasion."}
{"id": "PMID:1086063", "title": "Differentiation of a methylcholanthrene-induced sarcoma to a benign plexiform fibroneural tumor in an adult frog (Rana pipiens). Possible influence of host regenerative capacity.", "content": "A spindle cell sarcoma appeared 20 months after implantation of a pellet of 3-methylcholanthrene in the denervated foreleg of an adult frog, Rana pipiens. Its growth rate and cellular structure were observed over the subsequent 19 months, the former remaining constant for the first 14 months, then slackening markedly during the final 4 months. Serial biopsies disclosed maturation to a well-differentiated fibroneural tumor of benign appearance, the change taking place notably during the period of decelerated growth rate. An autotransplant of the tumor to the hind leg adopted the same growth pattern and maturation. Possible causes for this behavior are discussed.", "contents": "Differentiation of a methylcholanthrene-induced sarcoma to a benign plexiform fibroneural tumor in an adult frog (Rana pipiens). Possible influence of host regenerative capacity. A spindle cell sarcoma appeared 20 months after implantation of a pellet of 3-methylcholanthrene in the denervated foreleg of an adult frog, Rana pipiens. Its growth rate and cellular structure were observed over the subsequent 19 months, the former remaining constant for the first 14 months, then slackening markedly during the final 4 months. Serial biopsies disclosed maturation to a well-differentiated fibroneural tumor of benign appearance, the change taking place notably during the period of decelerated growth rate. An autotransplant of the tumor to the hind leg adopted the same growth pattern and maturation. Possible causes for this behavior are discussed."}
{"id": "PMID:1086064", "title": "Ultrastructural study of Reed-Sternberg cells. Comparison with transformed lymphocytes and histiocytes.", "content": "The ultrastructural features of Reed-Sternberg cells from 17 patients with Hodgkin's disease were compared with those of histiocytes and transformed lymphocytes in both benign and malignant conditions. Transformed lymphocytes and Reed-Sternberg cells appeared to have similar features, including large nuclei with dispersed chromatin, large nucleoli, and great numbers of cytoplasmic polyribosomes. Histiocytes contained abundant cytoplasmic lysosomal granules and microfilaments. These results are indicative of the origin of Reed-Sternberg cells from lymphocytes.", "contents": "Ultrastructural study of Reed-Sternberg cells. Comparison with transformed lymphocytes and histiocytes. The ultrastructural features of Reed-Sternberg cells from 17 patients with Hodgkin's disease were compared with those of histiocytes and transformed lymphocytes in both benign and malignant conditions. Transformed lymphocytes and Reed-Sternberg cells appeared to have similar features, including large nuclei with dispersed chromatin, large nucleoli, and great numbers of cytoplasmic polyribosomes. Histiocytes contained abundant cytoplasmic lysosomal granules and microfilaments. These results are indicative of the origin of Reed-Sternberg cells from lymphocytes."}
{"id": "PMID:1086066", "title": "Vasopressin control of massive hemorrhage in chronic ulcerative colitis.", "content": "A case of a massive colonic hemorrhage in nontoxic, quiescent ulcerative colitis is described. The source of active colonic bleeding was primarily defined with selective superior mesenteric arteriography and was completely controlled with transcatheter vasopressin infusion. A suubsequent elective segmental distal transverse and descending colectomy revealed chronic ulcerative colitis; localized marked inflammatory giant pseudopolyp formation near the splenic flexure was responsible for the bleeding.", "contents": "Vasopressin control of massive hemorrhage in chronic ulcerative colitis. A case of a massive colonic hemorrhage in nontoxic, quiescent ulcerative colitis is described. The source of active colonic bleeding was primarily defined with selective superior mesenteric arteriography and was completely controlled with transcatheter vasopressin infusion. A suubsequent elective segmental distal transverse and descending colectomy revealed chronic ulcerative colitis; localized marked inflammatory giant pseudopolyp formation near the splenic flexure was responsible for the bleeding."}
{"id": "PMID:1086079", "title": "Abnormal lymphocyte function is secondary to drug-induced autoimmunity.", "content": "Abnormal lymphocyte function has been frequently reported in patients with connective tissue diseases but its significance has been uncertain. Sequential studies of lymphocyte function were carried out in patients receiving the beta-adrenergic blocking drug practolol (Eraldin) both before and during the development of autoimmune complications. No evidence was obtained that abnormal lymphocyte function presaged the onset of autoimmunity, and when these tests did show deficient responses these could be correlated with disease activity in general.", "contents": "Abnormal lymphocyte function is secondary to drug-induced autoimmunity. Abnormal lymphocyte function has been frequently reported in patients with connective tissue diseases but its significance has been uncertain. Sequential studies of lymphocyte function were carried out in patients receiving the beta-adrenergic blocking drug practolol (Eraldin) both before and during the development of autoimmune complications. No evidence was obtained that abnormal lymphocyte function presaged the onset of autoimmunity, and when these tests did show deficient responses these could be correlated with disease activity in general."}
{"id": "PMID:1086080", "title": "Circumflex coronary artery bypass grafting via transverse sinus.", "content": "Grafting the circumflex coronary artery has been more difficult technically than the right or anterior descending because of its posterior location and the angles of its marginal branches. However, grafting this artery has been substantially simplified by a method wherein the graft is passed directly through the transverse sinus and anastomosed to the back of the ascending aorta. This follows the most direct course to the ascending aorta and the graft is much shorter than one brought anterior to the heart. There is little chance of kinking the graft, since its angle of attachment is ideal, and due to the anatomical configuration of the transverse sinus, there is more room for the graft and compression is unlikely. This allows more room on the anterior surface of the aorta for grafts to other coronary arteries. This method has been used in 50 cases and blood flows have been excellent. Twelve of these cases have been restudied postoperatively and the grafts are patent.", "contents": "Circumflex coronary artery bypass grafting via transverse sinus. Grafting the circumflex coronary artery has been more difficult technically than the right or anterior descending because of its posterior location and the angles of its marginal branches. However, grafting this artery has been substantially simplified by a method wherein the graft is passed directly through the transverse sinus and anastomosed to the back of the ascending aorta. This follows the most direct course to the ascending aorta and the graft is much shorter than one brought anterior to the heart. There is little chance of kinking the graft, since its angle of attachment is ideal, and due to the anatomical configuration of the transverse sinus, there is more room for the graft and compression is unlikely. This allows more room on the anterior surface of the aorta for grafts to other coronary arteries. This method has been used in 50 cases and blood flows have been excellent. Twelve of these cases have been restudied postoperatively and the grafts are patent."}
{"id": "PMID:1086081", "title": "The problem-oriented medical record: evaluation and management of anemia before and during use.", "content": "The problem-oriented medical record (POMR) is designed to rationalize and improve the structure of the medical record. This change might be expected to enhance the outcome of patient care. Therefore, I assessed the effect of the POMR on the care of the problem--anemia--in a Veterans Administration teaching hospital. The POMR concept was introduced by lectures and supported by chart audit. Four months later, the care described in 29 sequential records containing the discharge diagnosis--anemia--was compared with 31 records produced at the same period in the previous year by house officers not using the POMR. Records were graded for maintenance of the POMR format. Completeness of the \"data base,\" as well as time to problem identification, therapy, and diagnostic description were compared. Introduction of the POMR had no observable beneficial effect on the thoroughness of data base accumulation or the process of patient care for the problem (anemia) or, in another study, on the ease or accuracy of chart audit.", "contents": "The problem-oriented medical record: evaluation and management of anemia before and during use. The problem-oriented medical record (POMR) is designed to rationalize and improve the structure of the medical record. This change might be expected to enhance the outcome of patient care. Therefore, I assessed the effect of the POMR on the care of the problem--anemia--in a Veterans Administration teaching hospital. The POMR concept was introduced by lectures and supported by chart audit. Four months later, the care described in 29 sequential records containing the discharge diagnosis--anemia--was compared with 31 records produced at the same period in the previous year by house officers not using the POMR. Records were graded for maintenance of the POMR format. Completeness of the \"data base,\" as well as time to problem identification, therapy, and diagnostic description were compared. Introduction of the POMR had no observable beneficial effect on the thoroughness of data base accumulation or the process of patient care for the problem (anemia) or, in another study, on the ease or accuracy of chart audit."}
{"id": "PMID:1086082", "title": "Histiocytosis X and compulsive water drinking: report of a case.", "content": "We describe a patient with histiocytosis X and compulsive water drinking. The association of diabetes insipidus with histiocytosis X is well recognized, and this patient was initially considered to have diabetes insipidus. It was only after further testing that the proper diagnosis was made.", "contents": "Histiocytosis X and compulsive water drinking: report of a case. We describe a patient with histiocytosis X and compulsive water drinking. The association of diabetes insipidus with histiocytosis X is well recognized, and this patient was initially considered to have diabetes insipidus. It was only after further testing that the proper diagnosis was made."}
{"id": "PMID:1086083", "title": "Dynamics of nucleic acids and total proteins in thymus of Walker carcinosarcoma bearing rats.", "content": "Thymus responses in tumoral-bearing hosts can illustrate two moments of immunological crisis corresponding to the tumour intake and its metastazic spread. These data are in accord with Burnet's (3) hypothesis that cancer is due to the inability of immunological surveillance mechanisms to reject somatic cells bearing neoplastic mutation.", "contents": "Dynamics of nucleic acids and total proteins in thymus of Walker carcinosarcoma bearing rats. Thymus responses in tumoral-bearing hosts can illustrate two moments of immunological crisis corresponding to the tumour intake and its metastazic spread. These data are in accord with Burnet's (3) hypothesis that cancer is due to the inability of immunological surveillance mechanisms to reject somatic cells bearing neoplastic mutation."}
{"id": "PMID:1086086", "title": "A morphological study of human vestibular sensory epithelia.", "content": "Surface preparations were made of the vestibular sense organs of humans from membranous labyrinths removed within ten hours of death. Total sensory cell counts and surface area measurements were made from composite photographic reconstructions. The general topographical and cytoarchitectural arrangements were found to be similar to those previously described for the guinea pig and squirrel monkey. The surface areas and sensory cell populations of the human cristae were only slightly greater than those recorded for these animals; however, the figures for the human maculae were twofold to fourfold greater. It seems possible that this exceptional enlargement of the sensory epithelia of the static labyrinth is in response to a physiological need generated by the evolutionary transition of primates from the quadruped to the biped stance.", "contents": "A morphological study of human vestibular sensory epithelia. Surface preparations were made of the vestibular sense organs of humans from membranous labyrinths removed within ten hours of death. Total sensory cell counts and surface area measurements were made from composite photographic reconstructions. The general topographical and cytoarchitectural arrangements were found to be similar to those previously described for the guinea pig and squirrel monkey. The surface areas and sensory cell populations of the human cristae were only slightly greater than those recorded for these animals; however, the figures for the human maculae were twofold to fourfold greater. It seems possible that this exceptional enlargement of the sensory epithelia of the static labyrinth is in response to a physiological need generated by the evolutionary transition of primates from the quadruped to the biped stance."}
{"id": "PMID:1086087", "title": "A clinical study on the ototoxic effects of tobramycin.", "content": "In a clinical, prospective, non-randomized study, tobramycin has shown its high antibacterial activity in Gram-negative bacillary infections. The incidence of ototoxic side effects was very low: none of 18 patients studied before, at the end and 9 months after completion of tobramycin therapy showed any change in pure tone audiograms. Five of these 18 patients had renal impairment: 3 of them developed subjective and objective evidence of vestibular dysfunction, as demonstrated by caloric vestibulometry. However, these bilateral vestibular lesions all proved to be reversible a few weeks after cessation of therapy. Despite the low incidence and the reversibility of the ototoxic side effects, it seems advisable to monitor patients on long-duration tobramycin therapy for signs of vestibular dysfunction, particularly in cases of renal insufficiency.", "contents": "A clinical study on the ototoxic effects of tobramycin. In a clinical, prospective, non-randomized study, tobramycin has shown its high antibacterial activity in Gram-negative bacillary infections. The incidence of ototoxic side effects was very low: none of 18 patients studied before, at the end and 9 months after completion of tobramycin therapy showed any change in pure tone audiograms. Five of these 18 patients had renal impairment: 3 of them developed subjective and objective evidence of vestibular dysfunction, as demonstrated by caloric vestibulometry. However, these bilateral vestibular lesions all proved to be reversible a few weeks after cessation of therapy. Despite the low incidence and the reversibility of the ototoxic side effects, it seems advisable to monitor patients on long-duration tobramycin therapy for signs of vestibular dysfunction, particularly in cases of renal insufficiency."}
{"id": "PMID:1086089", "title": "Linear densities in mitochondria of human myocardial cells.", "content": "Ultrastructural study of myocardial biopsies from 5 patients who underwent myocardial revascularization procedures because of impending infarction showed mitochondria with linear densities. These densities, which occupied the intracristal space, appeared to have a high protein but low mineral content. It is suggested that linear densities may be an expression of severe, acute myocardial cell injury.", "contents": "Linear densities in mitochondria of human myocardial cells. Ultrastructural study of myocardial biopsies from 5 patients who underwent myocardial revascularization procedures because of impending infarction showed mitochondria with linear densities. These densities, which occupied the intracristal space, appeared to have a high protein but low mineral content. It is suggested that linear densities may be an expression of severe, acute myocardial cell injury."}
{"id": "PMID:1086088", "title": "The inheritance of affective disorders: a review of data and of hypotheses.", "content": "A genetic factor in affective disorders is suggested by twin and family history studies. The form of disorder (BP or UP) is transmitted within families. Early onset of affective disorder is associated with increased morbid risk of the disorder in relatives, but age at onset is not itself a transmitted factor. Female relatives have higher prevalence of illness, but sex of the ill person does not appear to be a factor in transmission. Genetic models of multifactorial or single-gene autosomal inheritance are compatible with some but not all of the family history studies reported. The hypothesis of sex-linked transmission of BP illness has been proposed, and some pedigrees compatible with X-linkage have been reported, but family studies do not suggest that this is generally present. Other possible modes of inheritance remain to be tested. Investigative strategies for identification of the \"affective genotype\" are discussed on the basis of biochemical, pharmacological, or other characteristics of persons with the disorders and their relatives, and on the basis of studies of known linkage markers.", "contents": "The inheritance of affective disorders: a review of data and of hypotheses. A genetic factor in affective disorders is suggested by twin and family history studies. The form of disorder (BP or UP) is transmitted within families. Early onset of affective disorder is associated with increased morbid risk of the disorder in relatives, but age at onset is not itself a transmitted factor. Female relatives have higher prevalence of illness, but sex of the ill person does not appear to be a factor in transmission. Genetic models of multifactorial or single-gene autosomal inheritance are compatible with some but not all of the family history studies reported. The hypothesis of sex-linked transmission of BP illness has been proposed, and some pedigrees compatible with X-linkage have been reported, but family studies do not suggest that this is generally present. Other possible modes of inheritance remain to be tested. Investigative strategies for identification of the \"affective genotype\" are discussed on the basis of biochemical, pharmacological, or other characteristics of persons with the disorders and their relatives, and on the basis of studies of known linkage markers."}
{"id": "PMID:1086090", "title": "Significance of angina pectoris in aortic valve stenosis.", "content": "Of 60 patients aged 45 to 66 years with aortic valve stenosis, 28 (47 per cent) had angina pectoris. Significant coronary arterial obstruction was shown by selective coronary cineangiography in 14 of them. Systolic pressure gradients across the aortic valve were lower in patients with angina than in those without. In those with angina, systolic gradients were higher in those with normal coronary arteriograms than in those with demonstrable coronary arterial disease. Aortic valve replacement relieved the angina in all patients who had normal coronary arteriograms. When valve replacement was combined with coronary bypass grafting in those with coronary arterial disease, surgical mortality was higher and symptomatic relief less predictable. Incapacitating angina in patients with aortic stenosis was nearly always associated with significant coronary disease. In those with less severe angina it was impossible to predict the state of the coronary arteries. Two patients, who did not have angina and who did not undergo coronary arteriography, died after aortic valve replacement and were found at necropsy to have unsuspected severe coronary disease. We, therefore, suggest that coronary arteriography should be carried out in all patients over the age of 40 years in whom surgery is being considered for aortic stenosis.", "contents": "Significance of angina pectoris in aortic valve stenosis. Of 60 patients aged 45 to 66 years with aortic valve stenosis, 28 (47 per cent) had angina pectoris. Significant coronary arterial obstruction was shown by selective coronary cineangiography in 14 of them. Systolic pressure gradients across the aortic valve were lower in patients with angina than in those without. In those with angina, systolic gradients were higher in those with normal coronary arteriograms than in those with demonstrable coronary arterial disease. Aortic valve replacement relieved the angina in all patients who had normal coronary arteriograms. When valve replacement was combined with coronary bypass grafting in those with coronary arterial disease, surgical mortality was higher and symptomatic relief less predictable. Incapacitating angina in patients with aortic stenosis was nearly always associated with significant coronary disease. In those with less severe angina it was impossible to predict the state of the coronary arteries. Two patients, who did not have angina and who did not undergo coronary arteriography, died after aortic valve replacement and were found at necropsy to have unsuspected severe coronary disease. We, therefore, suggest that coronary arteriography should be carried out in all patients over the age of 40 years in whom surgery is being considered for aortic stenosis."}
{"id": "PMID:1086091", "title": "Pathology of hearts after aortocoronary saphenous vein bypass grafting for coronary artery disease, studied by post-mortem coronary angiography.", "content": "A detailed pathological study was made in 10 patients dying up to 13 months after aortocoronary saphenous vein bypass grafting for coronary atherosclerosis. The coronary arteries and vein grafts were investigated by injection with a radio-opaque mass, radiography, dissection, and histology. The report is to some extent historical since the patients died during a period when the operation was first being introduced into two cardiothoracic hospitals. About 80 operations were performed during the time the 10 deaths occurred, a mortality of 12-5 per cent (including cases followed up to 13 months after operation). Seven of the patients were operated on for intractable angina and 3 with a view to aneurysmectomy. All the patients selected for operation were severely disabled despite medical treatment. The main cause of death was extremely severe coronary artery disease and its effects on the left ventricle; in one case, over two-thirds of the left ventricle had been destroyed by infarction before operation. Other causes or contributing causes of death were pulmonary embolism, myocardial infarction complicating angiography (ostial stenosis), and cerebral damage. Ten of the 14 vein grafts (71%) were patent at necropsy. A free flow of injection medium usually occurred between patent grafts and coronary arteries. Thrombosis of a graft was thought to have contributed to death in 3 patients, but not in a fourth who died of pulmonary embolism. Since thrombosis of grafts was usually secondary to poor run-off blood into severely atheromatous coronary arteries, this was also an indirect effect of the advanced coronary arterial disease. In one case, thrombosis followed severe chronic intimal thickening of a graft in place for 13 months. The study of these deaths emphasizes that in some patients the pathological changes in the coronary arteries and left ventricle are too severe for them to benefit from surgery. Vein grafts cannot be expected to distribute blood effectively through grossly narrowed coronary arteries. In addition, when a large part of the left ventricle is infarcted or scarred, it is almost certain that improving the blood supply by grafting will not result in significant regeneration of cardiac muscle. Since the time when this study was made, there have been few deaths among the many vein graft operations subsequently carried out in the hospitals involved. The two most important factors thought responsible for the improvement are the selection of cases more suitable for surgery by continued improvement of diagnostic techniques, and also the employment of more radical surgical procedures in the form of coronary endarterectomy and the insertion of more grafts per patient.", "contents": "Pathology of hearts after aortocoronary saphenous vein bypass grafting for coronary artery disease, studied by post-mortem coronary angiography. A detailed pathological study was made in 10 patients dying up to 13 months after aortocoronary saphenous vein bypass grafting for coronary atherosclerosis. The coronary arteries and vein grafts were investigated by injection with a radio-opaque mass, radiography, dissection, and histology. The report is to some extent historical since the patients died during a period when the operation was first being introduced into two cardiothoracic hospitals. About 80 operations were performed during the time the 10 deaths occurred, a mortality of 12-5 per cent (including cases followed up to 13 months after operation). Seven of the patients were operated on for intractable angina and 3 with a view to aneurysmectomy. All the patients selected for operation were severely disabled despite medical treatment. The main cause of death was extremely severe coronary artery disease and its effects on the left ventricle; in one case, over two-thirds of the left ventricle had been destroyed by infarction before operation. Other causes or contributing causes of death were pulmonary embolism, myocardial infarction complicating angiography (ostial stenosis), and cerebral damage. Ten of the 14 vein grafts (71%) were patent at necropsy. A free flow of injection medium usually occurred between patent grafts and coronary arteries. Thrombosis of a graft was thought to have contributed to death in 3 patients, but not in a fourth who died of pulmonary embolism. Since thrombosis of grafts was usually secondary to poor run-off blood into severely atheromatous coronary arteries, this was also an indirect effect of the advanced coronary arterial disease. In one case, thrombosis followed severe chronic intimal thickening of a graft in place for 13 months. The study of these deaths emphasizes that in some patients the pathological changes in the coronary arteries and left ventricle are too severe for them to benefit from surgery. Vein grafts cannot be expected to distribute blood effectively through grossly narrowed coronary arteries. In addition, when a large part of the left ventricle is infarcted or scarred, it is almost certain that improving the blood supply by grafting will not result in significant regeneration of cardiac muscle. Since the time when this study was made, there have been few deaths among the many vein graft operations subsequently carried out in the hospitals involved. The two most important factors thought responsible for the improvement are the selection of cases more suitable for surgery by continued improvement of diagnostic techniques, and also the employment of more radical surgical procedures in the form of coronary endarterectomy and the insertion of more grafts per patient."}
{"id": "PMID:1086097", "title": "Immunological aspects of psoriasis. II. Dissociated impairment of thymus-dependent lymphocytes.", "content": "We have investigated lymphocyte subpopulations (six different markers) and T cell functions (mitogen responses and serum thymic factor determination) in twenty patients with psoriasis compared with thirty-five healthy subjects. Normal results were found for B cell markers, but significantly lower numbers of T cells were assessed by E-rosettes and anti HTLA serum. Mitogen responses were significantly higher in psoriasis than in normal subjects of the same age. These findings could be in favour of a T cell subpopulation defect involved in an auto-immune pathogenesis of psoriasis.", "contents": "Immunological aspects of psoriasis. II. Dissociated impairment of thymus-dependent lymphocytes. We have investigated lymphocyte subpopulations (six different markers) and T cell functions (mitogen responses and serum thymic factor determination) in twenty patients with psoriasis compared with thirty-five healthy subjects. Normal results were found for B cell markers, but significantly lower numbers of T cells were assessed by E-rosettes and anti HTLA serum. Mitogen responses were significantly higher in psoriasis than in normal subjects of the same age. These findings could be in favour of a T cell subpopulation defect involved in an auto-immune pathogenesis of psoriasis."}
{"id": "PMID:1086098", "title": "Radioimmunoassay of human plasma trypsin.", "content": "A radioimmunoassay has been developed for the determination of human trypsin (3.4.21.4) in plasma. It allows the measurement of trypsin concentration in spite of the presence of plasma or pancreatic inhibitors. The human trypsin used as a standard and for labelling was isolated from pancreatic tissue and purified by affinity chromatography. The antiserum was obtained from guinea-pigs immunized with partially purified human trypsin. In the radioimmunoassay, the values of trypsin in serial dilutions of plasma were parallel to those of the standard curves. The assay was shown to be reproducible, sensitive and specific. However, the two antisera used did not distinguish between the enzyme and its proenzyme. In normal subjects, plasma values were found to be around 400 ng/ml. They were 10-40 times higher in patients with acute pancreatitis. The method appears to be much more specific for the diagnosis of acute pancreatitis than the current determinations of amylase and lipase activity.", "contents": "Radioimmunoassay of human plasma trypsin. A radioimmunoassay has been developed for the determination of human trypsin (3.4.21.4) in plasma. It allows the measurement of trypsin concentration in spite of the presence of plasma or pancreatic inhibitors. The human trypsin used as a standard and for labelling was isolated from pancreatic tissue and purified by affinity chromatography. The antiserum was obtained from guinea-pigs immunized with partially purified human trypsin. In the radioimmunoassay, the values of trypsin in serial dilutions of plasma were parallel to those of the standard curves. The assay was shown to be reproducible, sensitive and specific. However, the two antisera used did not distinguish between the enzyme and its proenzyme. In normal subjects, plasma values were found to be around 400 ng/ml. They were 10-40 times higher in patients with acute pancreatitis. The method appears to be much more specific for the diagnosis of acute pancreatitis than the current determinations of amylase and lipase activity."}
{"id": "PMID:1086099", "title": "Aromatic Tris-amidines. A new class of highly active inhibitors of trypsin-like proteases.", "content": "A number of novel aromatic Tris-amidines have been synthesized and investigated for their antiproteolytic property. The basic structure of the compounds is that of mesitylene where each of the methyl groups has been substituted with a 3- or 4-amidinophenoxy moiety. The compounds displayed considerable activity against trypsin (EC 3.4.21.4) and thrombin (EC 3.4.21.5), but proved most effective against porcine pancreatic kallikrein (EC 3.4.21.8). With this enzyme a Ki value of 2.43-10(-8) M was recorded for alpha,alpha',alpha''-tris(4-amidino-2-bromophenoxy)mesitylene at pH 8.1 and 37 degrees C. The most potent thrombin inhibitor, alpha,alpha',alpha''-tris(3-amidinophenoxy)mesitylene, had a Ki value of 6.51-10(-7) M and was also a strong overall anticoagulant. The inhibitors were able to interfere with the kinin release by human plasma kallikrein at concentrations as low as 1-10(-10) M. However, despite this remarkable antikallikrein effect and the known importance of plasma kallikrein in the activation of Hageman factor (factor XII), the compounds had only little influence on the early stages of blood coagulation.", "contents": "Aromatic Tris-amidines. A new class of highly active inhibitors of trypsin-like proteases. A number of novel aromatic Tris-amidines have been synthesized and investigated for their antiproteolytic property. The basic structure of the compounds is that of mesitylene where each of the methyl groups has been substituted with a 3- or 4-amidinophenoxy moiety. The compounds displayed considerable activity against trypsin (EC 3.4.21.4) and thrombin (EC 3.4.21.5), but proved most effective against porcine pancreatic kallikrein (EC 3.4.21.8). With this enzyme a Ki value of 2.43-10(-8) M was recorded for alpha,alpha',alpha''-tris(4-amidino-2-bromophenoxy)mesitylene at pH 8.1 and 37 degrees C. The most potent thrombin inhibitor, alpha,alpha',alpha''-tris(3-amidinophenoxy)mesitylene, had a Ki value of 6.51-10(-7) M and was also a strong overall anticoagulant. The inhibitors were able to interfere with the kinin release by human plasma kallikrein at concentrations as low as 1-10(-10) M. However, despite this remarkable antikallikrein effect and the known importance of plasma kallikrein in the activation of Hageman factor (factor XII), the compounds had only little influence on the early stages of blood coagulation."}
{"id": "PMID:1086100", "title": "Affinity chromatography of the uterine estradiol receptor on estradiol-PAB-cellulose: an artefact.", "content": "Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was undertaken, including the synthesis and testing of the radioactive material. Two main results were obtained: 1) Estradiol-PAB-cellulose is heavily contaminated with free ligand and releases it during the normal chromatographic conditions. 2) Estradiol spacer derivatives (hydroxyethylphenyl-diazo (2 or 4)-estradiol) have a very low affinity for the receptor (Ki = 10 muM). The conclusion is that estradiol-PAB-cellulose is unsuitable for affinity chromatography of estradiol receptor.", "contents": "Affinity chromatography of the uterine estradiol receptor on estradiol-PAB-cellulose: an artefact. Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was undertaken, including the synthesis and testing of the radioactive material. Two main results were obtained: 1) Estradiol-PAB-cellulose is heavily contaminated with free ligand and releases it during the normal chromatographic conditions. 2) Estradiol spacer derivatives (hydroxyethylphenyl-diazo (2 or 4)-estradiol) have a very low affinity for the receptor (Ki = 10 muM). The conclusion is that estradiol-PAB-cellulose is unsuitable for affinity chromatography of estradiol receptor."}
{"id": "PMID:1086102", "title": "The structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor III. Structure of the anhydro-trypsin-inhibitor complex.", "content": "The structure of the complex between anhydro-trypsin and pancreatic trypsin inhibitor has been determined by difference Fourier techniques using phases obtained from the native complex (Huber et al., 1974). It was refined independently by constrained crystallographic refinement at 1.9 A resolution. The anhydro-complex has Ser 195 converted to dehydro-alanine. There were no other significant structural changes. In particular, the high degree of pyramidalization of the C atom of Lys 15 (I) of the inhibitor component observed in the native complex in maintained in the anhydro-species.", "contents": "The structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor III. Structure of the anhydro-trypsin-inhibitor complex. The structure of the complex between anhydro-trypsin and pancreatic trypsin inhibitor has been determined by difference Fourier techniques using phases obtained from the native complex (Huber et al., 1974). It was refined independently by constrained crystallographic refinement at 1.9 A resolution. The anhydro-complex has Ser 195 converted to dehydro-alanine. There were no other significant structural changes. In particular, the high degree of pyramidalization of the C atom of Lys 15 (I) of the inhibitor component observed in the native complex in maintained in the anhydro-species."}
{"id": "PMID:1086108", "title": "Human lymphocyte motility: normal characteristics and anomalous behavior of chronic lymphocytic leukemia cells.", "content": "The characteristics of human lymphocyte motility and its relationship to the redistribution of surface membrane antigens (capping) are poorly defined. Since chronic lymphocytic leukemia (CLL) cells cap poorly when compared with normal human lymphocytes, this study was undertaken to compare the motility of these two cell types. A modification of the Boyden chamber system was employed to quantify lymphocyte motility by placing lymphocyte suspensions on 8-mum convoluted-pore nitrocellulose filters and measuring the depth of migration of the cells into the filter at 37 degrees C. After 3 hr of incubation, CLL cells migrated significantly less into the filter than normal cells. Incubation in the presence of sodium azide or at 4 degrees C abolished all motility, indicating the active nature of the process. The relative motility of individual CLL patients' cells correlated best with the proportion of abnormal cells present as determined by surface receptor assays. The possibility that decreased cell motility in CLL was a reflection of enrichment by a \"bone marrow-derived\" (B cell) population was eliminated by the finding that normal B cells purified by gradient separation of rosetted cells migrated faster than normal T cells and considerably faster than CLL cells. Motility of normal and CLL lymphocytes was decreased by cytochalasin B and increased by colchicine, vincristine, and vinblastine. Thus, human lymphocyte motility appears to be dependent on microfilament integrity but not to require the colchicine-sensitive cytoskeleton. The decreased motility of CLL cells is the result of an intrinsic cell abnormality, but this finding cannot fully explain the decreased capping, since in human lymphocytes the latter is not prevented by an inhibitor of motility.", "contents": "Human lymphocyte motility: normal characteristics and anomalous behavior of chronic lymphocytic leukemia cells. The characteristics of human lymphocyte motility and its relationship to the redistribution of surface membrane antigens (capping) are poorly defined. Since chronic lymphocytic leukemia (CLL) cells cap poorly when compared with normal human lymphocytes, this study was undertaken to compare the motility of these two cell types. A modification of the Boyden chamber system was employed to quantify lymphocyte motility by placing lymphocyte suspensions on 8-mum convoluted-pore nitrocellulose filters and measuring the depth of migration of the cells into the filter at 37 degrees C. After 3 hr of incubation, CLL cells migrated significantly less into the filter than normal cells. Incubation in the presence of sodium azide or at 4 degrees C abolished all motility, indicating the active nature of the process. The relative motility of individual CLL patients' cells correlated best with the proportion of abnormal cells present as determined by surface receptor assays. The possibility that decreased cell motility in CLL was a reflection of enrichment by a \"bone marrow-derived\" (B cell) population was eliminated by the finding that normal B cells purified by gradient separation of rosetted cells migrated faster than normal T cells and considerably faster than CLL cells. Motility of normal and CLL lymphocytes was decreased by cytochalasin B and increased by colchicine, vincristine, and vinblastine. Thus, human lymphocyte motility appears to be dependent on microfilament integrity but not to require the colchicine-sensitive cytoskeleton. The decreased motility of CLL cells is the result of an intrinsic cell abnormality, but this finding cannot fully explain the decreased capping, since in human lymphocytes the latter is not prevented by an inhibitor of motility."}
{"id": "PMID:1086109", "title": "Colony growth of mouse bone marrow cells in agar contained in glass capillaries.", "content": "Mouse bone marrow cells were grown in semi-solid agar contained in glass capillary tubes. Several parameters affecting colony formation in the capillaries were studied. 10(4) cells in 100 mul incubation medium within one capillary produced 22 to 30 colonies of granulocytes and macrophages. Compared with the common petri dishes glass capillaries offer several advantages under the conditions used: 1. A twofold higher plating efficiency. 2. Applicability to optical scanning by light scattering and electronic counting, allowing automation and greatly improving sensitivity, statistical accuracy and reproducibility. Kinetics of colony growth can also be monitored. 3. Diminished risk of bacterial and fungal contamination. 4. A more than tenfold lower need for materials on similar statistical errors. Substituting methylcellulose for agar resulted in colonies of fibroblast-like cells adherent to glass surface. Glass non-adherent cells showed a threefold higher plating efficiency in agar.", "contents": "Colony growth of mouse bone marrow cells in agar contained in glass capillaries. Mouse bone marrow cells were grown in semi-solid agar contained in glass capillary tubes. Several parameters affecting colony formation in the capillaries were studied. 10(4) cells in 100 mul incubation medium within one capillary produced 22 to 30 colonies of granulocytes and macrophages. Compared with the common petri dishes glass capillaries offer several advantages under the conditions used: 1. A twofold higher plating efficiency. 2. Applicability to optical scanning by light scattering and electronic counting, allowing automation and greatly improving sensitivity, statistical accuracy and reproducibility. Kinetics of colony growth can also be monitored. 3. Diminished risk of bacterial and fungal contamination. 4. A more than tenfold lower need for materials on similar statistical errors. Substituting methylcellulose for agar resulted in colonies of fibroblast-like cells adherent to glass surface. Glass non-adherent cells showed a threefold higher plating efficiency in agar."}
{"id": "PMID:1086110", "title": "[Von Willebrand-J\u00fcrgens syndrome with a variant of factor VIII-associated antigen].", "content": "A family is described in which 5 out of 8 children had a marked bleeding disorder. The children showed prolonged bleeding times, abnormal platelet retention upon passage of blood through a glass bead column, the Willebrand factor activity as measured by ristocetin in a washed platelet system was low. Factor VIII/von Willebrand factor protein levels were normal even so the factor VIII-procoagulant activity. Even the parents and one child without any bleeding tendency and normal bleeding times had a reduced Willebrand factor activity. In all these patients evidence of an abnormal protein was observed on crossed antigen-antibody electrophoresis indicating a qualitative defect of the factor VIII/von Willebrand factor protein.", "contents": "[Von Willebrand-J\u00fcrgens syndrome with a variant of factor VIII-associated antigen]. A family is described in which 5 out of 8 children had a marked bleeding disorder. The children showed prolonged bleeding times, abnormal platelet retention upon passage of blood through a glass bead column, the Willebrand factor activity as measured by ristocetin in a washed platelet system was low. Factor VIII/von Willebrand factor protein levels were normal even so the factor VIII-procoagulant activity. Even the parents and one child without any bleeding tendency and normal bleeding times had a reduced Willebrand factor activity. In all these patients evidence of an abnormal protein was observed on crossed antigen-antibody electrophoresis indicating a qualitative defect of the factor VIII/von Willebrand factor protein."}
{"id": "PMID:1086111", "title": "A comparative study of the action of gamma-aminobutyric acid and piperazine on the lobster muscle fibre and the frog spinal cord.", "content": "1 The effects of gamma-aminobutyric acid (GABA) and piperazine were compared on two in vitro preparations, the lobster muscle fibre and the frog spinal cord. 2 Both GABA and piperazine increased the membrane conductance of single lobster muscle fibres without changing the membrane potential; sigmoidal log dose-conductance curves for these agents were obtained and a similar model expressed the receptor interaction of both substances. 3 The actions of GABA and piperazine on lobster muscle were antagonized by picrotoxin and were Cl-dependent. 4 In the frog spinal cord GABA depolarized the dorsal roots presumably by mimicking the activity of the transmitter depolarizing the primary afferents; sigmoidal log dose-response curves for GABA were obtained. 5 On the dorsal roots piperazine produced either depolarizations or biphasic responses; these were mainly indirect effects as was shown by experiments in the presence of tetrodotoxin (TTX). 6 The effects of GABA on the dorsal root (in TTX-treated cords) were antagonized by picrotoxin whereas those of piperazine were more resistant to this alkaloid. The GABA-induced responses appeared to be largely Na+-dependent while both Na+ and Cl- seemed to mediate the effects of piperazine. 7 It is proposed that piperazine has GABA-agonist activity on lobster muscle but little GABA-like activity on the frog spinal cord.", "contents": "A comparative study of the action of gamma-aminobutyric acid and piperazine on the lobster muscle fibre and the frog spinal cord. 1 The effects of gamma-aminobutyric acid (GABA) and piperazine were compared on two in vitro preparations, the lobster muscle fibre and the frog spinal cord. 2 Both GABA and piperazine increased the membrane conductance of single lobster muscle fibres without changing the membrane potential; sigmoidal log dose-conductance curves for these agents were obtained and a similar model expressed the receptor interaction of both substances. 3 The actions of GABA and piperazine on lobster muscle were antagonized by picrotoxin and were Cl-dependent. 4 In the frog spinal cord GABA depolarized the dorsal roots presumably by mimicking the activity of the transmitter depolarizing the primary afferents; sigmoidal log dose-response curves for GABA were obtained. 5 On the dorsal roots piperazine produced either depolarizations or biphasic responses; these were mainly indirect effects as was shown by experiments in the presence of tetrodotoxin (TTX). 6 The effects of GABA on the dorsal root (in TTX-treated cords) were antagonized by picrotoxin whereas those of piperazine were more resistant to this alkaloid. The GABA-induced responses appeared to be largely Na+-dependent while both Na+ and Cl- seemed to mediate the effects of piperazine. 7 It is proposed that piperazine has GABA-agonist activity on lobster muscle but little GABA-like activity on the frog spinal cord."}
{"id": "PMID:1086112", "title": "A comparative study of the effects of glutamate and kainate on the lobster muscle fibre and the frog spinal cord.", "content": "1 The depolarizing actions of glutamate and its conformationally restricted analogue kainate were investigated on the lobster muscle fibre and the frog spinal cord using intracellular and extracellular recordings, respectively. 2 Bath-applied kainate was less potent than glutamate on the lobster fibre but more potent on the frog cord. From the log-log transformation of dose-response curves it was proposed that more than one glutamate molecule was necessary to activate both the lobster and the frog receptor sites. In the frog, at least three kainate molecules were thought to be required for receptor activation. 3 The ionic dependence of glutamate and kainate responses appeared different for the two tissues. 4 Some possible explanations of the differential tissue sensitivity to kainate are discussed.", "contents": "A comparative study of the effects of glutamate and kainate on the lobster muscle fibre and the frog spinal cord. 1 The depolarizing actions of glutamate and its conformationally restricted analogue kainate were investigated on the lobster muscle fibre and the frog spinal cord using intracellular and extracellular recordings, respectively. 2 Bath-applied kainate was less potent than glutamate on the lobster fibre but more potent on the frog cord. From the log-log transformation of dose-response curves it was proposed that more than one glutamate molecule was necessary to activate both the lobster and the frog receptor sites. In the frog, at least three kainate molecules were thought to be required for receptor activation. 3 The ionic dependence of glutamate and kainate responses appeared different for the two tissues. 4 Some possible explanations of the differential tissue sensitivity to kainate are discussed."}
{"id": "PMID:1086113", "title": "Hypotension in rabbits infected with Trypanosoma brucei.", "content": "1 Blood pressures and heart rates of 12 anaesthetized rabbits chronically infected with T.brucei were measured (average infection time 39 days (range 25-67). The systolic BP was 31.4 +/- 5.7 mmHg, the diastolic BP 25.0 +/- 7.2 mmHg, and the heart rate 120.5 +/- 24.2 beats/minute. Two rabbits were already hypotensive 10 days after infection. In 12 anaesthetized control rabbits, the systolic BP was 66.2 +/- 7.3 mmHg (mean +/- s.e.), the diastolic BP 60.2 +/- 7.3 mmHg, and the heart rate 116.3 +/- 15.9 beats/minute. 2 The intravenous injection of 3 X 10(8) disintegrated trypanosomes into infected rabbits lowered the blood pressure by 41.4 +/- 22.0%. Pretreatment of two rabbits with aprotinin prior to administration of parasites prevented the fall in blood pressure. 3 Injection of 3 X 10(8) live trypanosomes complexed with hyperimmune sera produced a fall of 68.3 +/- 38.4% in the systolic BP of normal rabbits. Disintegrated or live trypanosomes, or hyperimmune sera alone had no effect. Pretreatment of animals with aprotinin prior to administration of the immune complex abolished the fall in BP. 4 The results suggest that the profound hypotension in chronic trypanosomiasis is caused by complex formation of trypanosomes with antibody. Since it can be prevented by pretreatment with aprotinin, it is likely that activation of plasma kallikrein with a subsequent release of plasma kinins contributes to this effect.", "contents": "Hypotension in rabbits infected with Trypanosoma brucei. 1 Blood pressures and heart rates of 12 anaesthetized rabbits chronically infected with T.brucei were measured (average infection time 39 days (range 25-67). The systolic BP was 31.4 +/- 5.7 mmHg, the diastolic BP 25.0 +/- 7.2 mmHg, and the heart rate 120.5 +/- 24.2 beats/minute. Two rabbits were already hypotensive 10 days after infection. In 12 anaesthetized control rabbits, the systolic BP was 66.2 +/- 7.3 mmHg (mean +/- s.e.), the diastolic BP 60.2 +/- 7.3 mmHg, and the heart rate 116.3 +/- 15.9 beats/minute. 2 The intravenous injection of 3 X 10(8) disintegrated trypanosomes into infected rabbits lowered the blood pressure by 41.4 +/- 22.0%. Pretreatment of two rabbits with aprotinin prior to administration of parasites prevented the fall in blood pressure. 3 Injection of 3 X 10(8) live trypanosomes complexed with hyperimmune sera produced a fall of 68.3 +/- 38.4% in the systolic BP of normal rabbits. Disintegrated or live trypanosomes, or hyperimmune sera alone had no effect. Pretreatment of animals with aprotinin prior to administration of the immune complex abolished the fall in BP. 4 The results suggest that the profound hypotension in chronic trypanosomiasis is caused by complex formation of trypanosomes with antibody. Since it can be prevented by pretreatment with aprotinin, it is likely that activation of plasma kallikrein with a subsequent release of plasma kinins contributes to this effect."}
{"id": "PMID:1086114", "title": "Possible influence of intrarenal generation of kinins on prostaglandin release from the rabbit perfused kidney.", "content": "The effects of bradykinin and kininogen on renal prostaglandin release were studied in rabbit isolated kidneys perfused with oxygenated Krebs solution. The concentration of prostaglandin-like material in kidney effluent was determined by bioassay after extraction of the samples with organic solvents. In 7 experiments the samples were assayed after separation of prostaglandins E and F by thin layer chromatography. Addition of bradykinin to the perfusing fluid increased the venous and urinary effluxes of prostaglandin E-like substance by sixfold and fivefold, respectively, but efflux of prostaglandin F-like material was unaffected. Addition of kininogen to the perfusing fluid augmented the venous and urinary release of prostaglandin E-like substances by fifteenfold and ninefold respectively and caused a twofold increase in the efflux of prostaglandin F-like material into the venous effluent. Aprotinin, a kallikrein inhibitor, reduced the prostaglandin releasing action of kininogen but not of bradykinin. In contrast, inhibition of prostaglandin synthesis by indomethacin suppressed the release of prostaglandin evoked by either bradykinin or kininogen. This study suggests that augmented release of prostaglandins in response to kininogen is a consequence of renal generation of kinins. Thus, changes in the intrarenal activity of the kallikreinkinin system may modulate renal prostaglandin release.", "contents": "Possible influence of intrarenal generation of kinins on prostaglandin release from the rabbit perfused kidney. The effects of bradykinin and kininogen on renal prostaglandin release were studied in rabbit isolated kidneys perfused with oxygenated Krebs solution. The concentration of prostaglandin-like material in kidney effluent was determined by bioassay after extraction of the samples with organic solvents. In 7 experiments the samples were assayed after separation of prostaglandins E and F by thin layer chromatography. Addition of bradykinin to the perfusing fluid increased the venous and urinary effluxes of prostaglandin E-like substance by sixfold and fivefold, respectively, but efflux of prostaglandin F-like material was unaffected. Addition of kininogen to the perfusing fluid augmented the venous and urinary release of prostaglandin E-like substances by fifteenfold and ninefold respectively and caused a twofold increase in the efflux of prostaglandin F-like material into the venous effluent. Aprotinin, a kallikrein inhibitor, reduced the prostaglandin releasing action of kininogen but not of bradykinin. In contrast, inhibition of prostaglandin synthesis by indomethacin suppressed the release of prostaglandin evoked by either bradykinin or kininogen. This study suggests that augmented release of prostaglandins in response to kininogen is a consequence of renal generation of kinins. Thus, changes in the intrarenal activity of the kallikreinkinin system may modulate renal prostaglandin release."}
{"id": "PMID:1086115", "title": "Control of duodenal haemorrhage with cyanoacrylate.", "content": "Three cases of massive duodenal haemorrhage refractory to conventional therapy have been successfully treated by percutaneous selective intra-arterial injection of Isobutyl 2 cyanoacrylate. Case histories, injection technique and review of the literature are presented.", "contents": "Control of duodenal haemorrhage with cyanoacrylate. Three cases of massive duodenal haemorrhage refractory to conventional therapy have been successfully treated by percutaneous selective intra-arterial injection of Isobutyl 2 cyanoacrylate. Case histories, injection technique and review of the literature are presented."}
{"id": "PMID:1086117", "title": "Prospective comparison of double-contrast barium meal examination and fibreoptic endoscopy in acute upper gastrointestinal haemorrhage.", "content": "Fibre-optic endoscopy was compared prospectively with double-contrast radiology in 53 consecutive patients admitted with acute gastrointestinal haemorrhage. The bleeding site was correctly identified by endoscopy in 94% of patients and the final diagnosis was correctly given in 89%. The corresponding figures with radiology were 83% and 74%. Among the 50 patients with a final diagnosis of a bleeding site in the upper gastrointestinal tract endoscopy indicated the site of bleeding in all and radiology indicated it in 88%. Both investigations were well tolerated by patients. Endoscopy is the investigation of choice, but when it is not available double-contrast radiology will show the site of bleeding in 80-90% of patients.", "contents": "Prospective comparison of double-contrast barium meal examination and fibreoptic endoscopy in acute upper gastrointestinal haemorrhage. Fibre-optic endoscopy was compared prospectively with double-contrast radiology in 53 consecutive patients admitted with acute gastrointestinal haemorrhage. The bleeding site was correctly identified by endoscopy in 94% of patients and the final diagnosis was correctly given in 89%. The corresponding figures with radiology were 83% and 74%. Among the 50 patients with a final diagnosis of a bleeding site in the upper gastrointestinal tract endoscopy indicated the site of bleeding in all and radiology indicated it in 88%. Both investigations were well tolerated by patients. Endoscopy is the investigation of choice, but when it is not available double-contrast radiology will show the site of bleeding in 80-90% of patients."}
{"id": "PMID:1086118", "title": "Histocompatibility antigens, autoantibodies, and immunoglobulins in alcoholic liver disease.", "content": "Determination of histocompatibility antigens in 63 patients with alcoholic liver disease showed that HLA-B8 was more prevalent in patients with cirrhosis than in controls, but among those with fatty liver and minimal fibrosis the prevalence of this antigen was normal. Another noticeable difference was the absence of HLAA28 in the cirrhotic group. In the total series of 219 patients the prevalence of antinuclear and smooth muscle antibodies was raised; they were especially prevalent in patients with cirrhosis. Raised serum IgA and IgG concentrations were also common (found in 50% and 37% respectively) and were again significantly associated with cirrhosis. In contrast, serum IgM levels, which were raised in 46% of cases, were not significantly related to the presence of cirrhosis but correlated significantly with the degree of portacaval shunting. These results support recent evidence suggesting that immune responses may be implicated in alcohol-induced liver damage, particularly in its progression to cirrhosis.", "contents": "Histocompatibility antigens, autoantibodies, and immunoglobulins in alcoholic liver disease. Determination of histocompatibility antigens in 63 patients with alcoholic liver disease showed that HLA-B8 was more prevalent in patients with cirrhosis than in controls, but among those with fatty liver and minimal fibrosis the prevalence of this antigen was normal. Another noticeable difference was the absence of HLAA28 in the cirrhotic group. In the total series of 219 patients the prevalence of antinuclear and smooth muscle antibodies was raised; they were especially prevalent in patients with cirrhosis. Raised serum IgA and IgG concentrations were also common (found in 50% and 37% respectively) and were again significantly associated with cirrhosis. In contrast, serum IgM levels, which were raised in 46% of cases, were not significantly related to the presence of cirrhosis but correlated significantly with the degree of portacaval shunting. These results support recent evidence suggesting that immune responses may be implicated in alcohol-induced liver damage, particularly in its progression to cirrhosis."}
{"id": "PMID:1086122", "title": "Serotonin involvement in the blockade of bulbospinal inhibition of the spinal monosynaptic reflex.", "content": "Bulbospinal inhibition of the extensor quadriceps monosynaptic reflex (MSR) was antagonized by the serotonin precursor, 5-hydroxytryptophan (5-HTP, 75 mg/kg), in unanesthetized, mid-collicular, decerebrate cats. Fluoxetine HCl (Lilly 110140, 0.25 - 6 mg/kg), a specific serotonin neuronal uptake blocker, also blocked this inhibition as well as bulbospinal inhibition of the flexor posterior biceps-semi-tendinosus MSR. The serotonin antagonist, cyproheptadine HCl (5 mg/kg), partially reversed the above blocking actions of 5-HTP and fluoxetine and enhanced bulbospinal inhibition when administered alone in doses of 2.5-5 mg/kg. Imipramine HCl (0.125 - 4 mg/kg) was more potent in antagonizing bulbospinal inhibition of the dorsal root-ventral root MSR when administered intra-arterially to the spinal cord than when injected intra-arterially to the brain stem or intravenously, indicating that the spinal cord is the site of imipramine's action. These results support our earlier proposal that a 5-HT system antagonizes bulbospinal inhibition of the MSR. They also indicate that the 5-HT system is tonically active and exerts its blocking action in the spinal cord.", "contents": "Serotonin involvement in the blockade of bulbospinal inhibition of the spinal monosynaptic reflex. Bulbospinal inhibition of the extensor quadriceps monosynaptic reflex (MSR) was antagonized by the serotonin precursor, 5-hydroxytryptophan (5-HTP, 75 mg/kg), in unanesthetized, mid-collicular, decerebrate cats. Fluoxetine HCl (Lilly 110140, 0.25 - 6 mg/kg), a specific serotonin neuronal uptake blocker, also blocked this inhibition as well as bulbospinal inhibition of the flexor posterior biceps-semi-tendinosus MSR. The serotonin antagonist, cyproheptadine HCl (5 mg/kg), partially reversed the above blocking actions of 5-HTP and fluoxetine and enhanced bulbospinal inhibition when administered alone in doses of 2.5-5 mg/kg. Imipramine HCl (0.125 - 4 mg/kg) was more potent in antagonizing bulbospinal inhibition of the dorsal root-ventral root MSR when administered intra-arterially to the spinal cord than when injected intra-arterially to the brain stem or intravenously, indicating that the spinal cord is the site of imipramine's action. These results support our earlier proposal that a 5-HT system antagonizes bulbospinal inhibition of the MSR. They also indicate that the 5-HT system is tonically active and exerts its blocking action in the spinal cord."}
{"id": "PMID:1086127", "title": "Aortocoronary bypass made possible by coronary endarterectomy.", "content": "Thirty bypass grafts to coronary arteries were made possible by manual core endarterectomy. The overall patency rate of the grafts was 57%, which is less than the patency rate of bypass grafts to nonendarterectomized vessels (75 to 80%). In the perioperative period and during follow-up to 29 months there was no increase in mortality or morbidity even when the endarterectomized vessel subsequently became occluded. These results represent early technical experience. Routine anticoagulant therapy in the postoperative period, to prevent early occlusion, was not used. The results of this and other studies suggest that coronary endarterectomy with bypass grafting is a useful procedure in situations where the coronary artery is so severely obstructed that standard saphenous vein bypass grafting cannot be performed; the procedure is superior to coronary endarterectomy alone.", "contents": "Aortocoronary bypass made possible by coronary endarterectomy. Thirty bypass grafts to coronary arteries were made possible by manual core endarterectomy. The overall patency rate of the grafts was 57%, which is less than the patency rate of bypass grafts to nonendarterectomized vessels (75 to 80%). In the perioperative period and during follow-up to 29 months there was no increase in mortality or morbidity even when the endarterectomized vessel subsequently became occluded. These results represent early technical experience. Routine anticoagulant therapy in the postoperative period, to prevent early occlusion, was not used. The results of this and other studies suggest that coronary endarterectomy with bypass grafting is a useful procedure in situations where the coronary artery is so severely obstructed that standard saphenous vein bypass grafting cannot be performed; the procedure is superior to coronary endarterectomy alone."}
{"id": "PMID:1086130", "title": "Tumor-caused changes in host protein synthesis under different dietary situations.", "content": "The effect of the AC33 tumor on protein synthesis in Lewis-Wistar rats was investigated under four different dietary regimens. The four diets used were: (a) 1.25 g amino acids plus 12.5 g glucose per day, (b) 1.25 g amino acids, (c) 1.25 g glucose per day, and (d) 12.5 g glucose per day. The rats were maintained on these four diets for 4 days. On the 5th day, 65 to 75 mg 99.2% [15N] glycine were added to the infusate and infused at a constant rate for the next 18 hr. The rats were then sacrificed and the liver, lung, heart, kidney, anterior tibialis muscle, and tumor were rapidly removed and frozen in liquid nitrogen. The rate of protein synthesis for these tissues was calculated from the ratio of 15N in the tissue protein to that in the tissue intracellular fluid. The protein synthesis rates were compared with the values found for a series of nontumor control rats fed the same diets. Relative to the control rats, muscle protein synthesis decreased on Diet 1, and liver protein synthesis increased with the three deficient diets.", "contents": "Tumor-caused changes in host protein synthesis under different dietary situations. The effect of the AC33 tumor on protein synthesis in Lewis-Wistar rats was investigated under four different dietary regimens. The four diets used were: (a) 1.25 g amino acids plus 12.5 g glucose per day, (b) 1.25 g amino acids, (c) 1.25 g glucose per day, and (d) 12.5 g glucose per day. The rats were maintained on these four diets for 4 days. On the 5th day, 65 to 75 mg 99.2% [15N] glycine were added to the infusate and infused at a constant rate for the next 18 hr. The rats were then sacrificed and the liver, lung, heart, kidney, anterior tibialis muscle, and tumor were rapidly removed and frozen in liquid nitrogen. The rate of protein synthesis for these tissues was calculated from the ratio of 15N in the tissue protein to that in the tissue intracellular fluid. The protein synthesis rates were compared with the values found for a series of nontumor control rats fed the same diets. Relative to the control rats, muscle protein synthesis decreased on Diet 1, and liver protein synthesis increased with the three deficient diets."}
{"id": "PMID:1086131", "title": "Active T-rosette-forming cells in the peripheral blood of cancer patients.", "content": "We  studied a subpopulation of the thymus-dependent rosette-forming lymphocytes from the peripheral blood of normal individuals and of untreated patients with solid tumors or hematological cancers. This subpopulation of the thymus-dependent rosette-forming cells (T-RFC), termed the \"active T-RFC,\" may be relatively more immunocompetent than the total thymus-dependent population. The mean percentages and absolute numbers of active T-RFC of 40 healthy adult controls were 25.8 +/- 4.3 and 626 +/- 213, respectively. There was no difference in the percentage of active T-RFC between the controls (smokers and nonsmokers) and the 102 untreated patients with solid (localized or metastasized) tumors, 4 patients with Hodgkin's disease, or the 10 patients with non-Hodgkin's lymphomas. However, the absolute number of active T-RFC was significantly less in the cancer patients than in the controls. Eight patients with chronic lymphocytic leukemia had lower percentages but higher absolute numbers of active T-RFC, whereas 6 patients with multiple myeloma had higher percentage and lower absolute numbers than the controls. Following radiation therapy, 61 patients with solid tumors showed no difference in the percentage of active T-RFC, but the corresponding absolute numbers declined significantly. A good correlation was seen with patients having positive microbial skin test responses and normal percentage of active T-RFC. The significance of both the percentages and absolute numbers of active T-RFC and their relationship to patient status are discussed.", "contents": "Active T-rosette-forming cells in the peripheral blood of cancer patients. We  studied a subpopulation of the thymus-dependent rosette-forming lymphocytes from the peripheral blood of normal individuals and of untreated patients with solid tumors or hematological cancers. This subpopulation of the thymus-dependent rosette-forming cells (T-RFC), termed the \"active T-RFC,\" may be relatively more immunocompetent than the total thymus-dependent population. The mean percentages and absolute numbers of active T-RFC of 40 healthy adult controls were 25.8 +/- 4.3 and 626 +/- 213, respectively. There was no difference in the percentage of active T-RFC between the controls (smokers and nonsmokers) and the 102 untreated patients with solid (localized or metastasized) tumors, 4 patients with Hodgkin's disease, or the 10 patients with non-Hodgkin's lymphomas. However, the absolute number of active T-RFC was significantly less in the cancer patients than in the controls. Eight patients with chronic lymphocytic leukemia had lower percentages but higher absolute numbers of active T-RFC, whereas 6 patients with multiple myeloma had higher percentage and lower absolute numbers than the controls. Following radiation therapy, 61 patients with solid tumors showed no difference in the percentage of active T-RFC, but the corresponding absolute numbers declined significantly. A good correlation was seen with patients having positive microbial skin test responses and normal percentage of active T-RFC. The significance of both the percentages and absolute numbers of active T-RFC and their relationship to patient status are discussed."}
{"id": "PMID:1086132", "title": "A radioimmunoassay for methotrexate and its comparison with spectrofluorimetric procedures.", "content": "A radioimmunoassay procedure has been developed for the direct measurement of methotrexate (MTX) in plasma, serum, cerebrospinal fluid, or urine samples. The assay is sensitive to levels of at least 100 pg of MTX and is highly specific for MTX in the presence of folinic acid (citrovorum factor), folic acid, tetrahydrogolic acid, and other folate analogs and known metabolites. Results from this procedure have been compared with those obtained with a spectrofluorimetric method, utilizing the plasma of cancer patients undergoing high-dose MTX treatment with citrovorum factor rescue. Results indicate that the method should be usful in the future in assisting individualization of dosage regimens and in the study of the pharmacokinetics and metabolism of MTX in cancer patients.", "contents": "A radioimmunoassay for methotrexate and its comparison with spectrofluorimetric procedures. A radioimmunoassay procedure has been developed for the direct measurement of methotrexate (MTX) in plasma, serum, cerebrospinal fluid, or urine samples. The assay is sensitive to levels of at least 100 pg of MTX and is highly specific for MTX in the presence of folinic acid (citrovorum factor), folic acid, tetrahydrogolic acid, and other folate analogs and known metabolites. Results from this procedure have been compared with those obtained with a spectrofluorimetric method, utilizing the plasma of cancer patients undergoing high-dose MTX treatment with citrovorum factor rescue. Results indicate that the method should be usful in the future in assisting individualization of dosage regimens and in the study of the pharmacokinetics and metabolism of MTX in cancer patients."}
{"id": "PMID:1086133", "title": "Fetal antigens in nonneoplastic conditions.", "content": "During studies that showed the presence of fetal antigens on the surface of human malignant melanoma tumor cells, polyvalent antisera specific for human fetal tissues of varying ages were developed. These reagents demonstrated varying patterns of expression of fetal antigens at different ages in various tissues of the human fetus. The possibility that nonneoplastic adult cells showing either maturation arrest or excessive proliferation also might express fetal antigens led to studies of human bone marrow. Although normal bone marrow cells expressed low levels of fetal antigens, large amounts were seen on bone marrow cells of patients with anemias due to iron, B12, or folic acid deficiencies, as well as on those with leukemia. Moreover, normal adult tissues adapted to long-term culture also expressed fetal antigens. After 3 weeks in organ culture adult human skin showed morphological changes similar to those seen in fetal periderm and strongly expressed fetal antigens. In addition, lymphoblasts in long-term cultured human lymphoid cell lines established from normal donors also carried surface fetal antigens. These latter antigens were shared with neoplastic B-cells (chronic lymphocytic leukemia) but not with T-cells. Their expression varied with the cell cycle. The reexpression of fetal antigens on malignant cells is thought to signal a basic derangement in the control of differentiation which is considered to be peculiar to neoplasia. However, these studies indicate that normal adult cells also may reexpress fetal antigens under circumstances unrelated to neoplasia but associated with either maturation arrest or rapid and excessive proliferation.", "contents": "Fetal antigens in nonneoplastic conditions. During studies that showed the presence of fetal antigens on the surface of human malignant melanoma tumor cells, polyvalent antisera specific for human fetal tissues of varying ages were developed. These reagents demonstrated varying patterns of expression of fetal antigens at different ages in various tissues of the human fetus. The possibility that nonneoplastic adult cells showing either maturation arrest or excessive proliferation also might express fetal antigens led to studies of human bone marrow. Although normal bone marrow cells expressed low levels of fetal antigens, large amounts were seen on bone marrow cells of patients with anemias due to iron, B12, or folic acid deficiencies, as well as on those with leukemia. Moreover, normal adult tissues adapted to long-term culture also expressed fetal antigens. After 3 weeks in organ culture adult human skin showed morphological changes similar to those seen in fetal periderm and strongly expressed fetal antigens. In addition, lymphoblasts in long-term cultured human lymphoid cell lines established from normal donors also carried surface fetal antigens. These latter antigens were shared with neoplastic B-cells (chronic lymphocytic leukemia) but not with T-cells. Their expression varied with the cell cycle. The reexpression of fetal antigens on malignant cells is thought to signal a basic derangement in the control of differentiation which is considered to be peculiar to neoplasia. However, these studies indicate that normal adult cells also may reexpress fetal antigens under circumstances unrelated to neoplasia but associated with either maturation arrest or rapid and excessive proliferation."}
{"id": "PMID:1086124", "title": "Olfactory receptor response characteristics: a factor analysis.", "content": "The responses to odor stimulation of 22 single olfactory units in the frog olfactory mucosa were recorded with metal filled micropipettes. Seven purified odorants matched in concentration and a pure air stimulus were administered. The change in firing frequency for each unit to each chemical was evaluated to determine: (1) whether the response patterns developed across all units are different from chemical to chemical and, (2) whether individual units can be grouped in terms of their similarity of their responses to all odorants. The results of a Bartlett Sphericity test suggest that each odorant produces an independent pattern of responses across units. To answer the second question, a factor analysis was employed. It examined the responses of each unit to all chemicals and yielded 7 out of a possible 22 independent factors, suggesting that there are 7 ways by which the units studied look at the odorant stimuli and an air control employed in this study. These analyses were evaluated and discussed in terms of previously published data suggesting receptor site specificity.", "contents": "Olfactory receptor response characteristics: a factor analysis. The responses to odor stimulation of 22 single olfactory units in the frog olfactory mucosa were recorded with metal filled micropipettes. Seven purified odorants matched in concentration and a pure air stimulus were administered. The change in firing frequency for each unit to each chemical was evaluated to determine: (1) whether the response patterns developed across all units are different from chemical to chemical and, (2) whether individual units can be grouped in terms of their similarity of their responses to all odorants. The results of a Bartlett Sphericity test suggest that each odorant produces an independent pattern of responses across units. To answer the second question, a factor analysis was employed. It examined the responses of each unit to all chemicals and yielded 7 out of a possible 22 independent factors, suggesting that there are 7 ways by which the units studied look at the odorant stimuli and an air control employed in this study. These analyses were evaluated and discussed in terms of previously published data suggesting receptor site specificity."}
{"id": "PMID:1086134", "title": "Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts.", "content": "Absorption studies demonstrate that T-cell lymphoma-associated antigens detected by rabbit antisera to human T-lymphoblast cell lines are present in suspensions of cord blood lymphocytes and phytohemagglutinin-stimulated adult blood lymphocytes in amounts similar to those found in T-cell lymphoma tumor cell suspensions. Smaller amounts of antigen activity are found in suspensions of tonsil cells, thymocytes, and unstimulated adult blood lymphocytes. Little or no antigen activity is found in suspensions of lymphoblasts from patients with other types of leukemia or from B-cell lines. T-cell depletion removes antigen activity from suspensions of normal lymphocytes. These findings suggest that T-cell lymphoma-associated antigens may be fetal antigens expressed by activated T-cells.", "contents": "Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts. Absorption studies demonstrate that T-cell lymphoma-associated antigens detected by rabbit antisera to human T-lymphoblast cell lines are present in suspensions of cord blood lymphocytes and phytohemagglutinin-stimulated adult blood lymphocytes in amounts similar to those found in T-cell lymphoma tumor cell suspensions. Smaller amounts of antigen activity are found in suspensions of tonsil cells, thymocytes, and unstimulated adult blood lymphocytes. Little or no antigen activity is found in suspensions of lymphoblasts from patients with other types of leukemia or from B-cell lines. T-cell depletion removes antigen activity from suspensions of normal lymphocytes. These findings suggest that T-cell lymphoma-associated antigens may be fetal antigens expressed by activated T-cells."}
{"id": "PMID:1086125", "title": "Effect of midbrain raph\u00e9 lesions or intracisternal 6-hydroxydopamine administration on D, L-5-hydroxytryptophan-14C metabolism after peripheral decarboxylase inhibition.", "content": "Brain and blood 14C-5-hydroxyindole metabolism was studied in normal, raph\u00e9-lesioned and 6-hydroxydopamine (60HDA)-treated rats given L-(-)-alpha-hydrazino-3, 4-dihydroxy-alpha-methylhydrocinnamic acid (MK-486) (50 mg/kg) intraperitoneally and 5-hydroxytryptophan (5-HTP)-14C (5 muCi, 0.51 mCi/mmol) intravenously. Raph\u00e9 lesioning and 60HDA treatment caused significant decrements in brain 5-hydroxytryptamine (5-HT)-14C and 5-hydroxyindoleacetic acid (5-HIAA)-14C levels and 5-HT concentrations compared to those in normal controls. In normal and 60HDA-treated rats there were decreased brain dopamine levels, possibly a consequence of non-specific metabolism of 5-HTP in brain. Blood 5-HIAA-14C levels were significantly decreased in 60HDA-treated but not raph\u00e9-lesioned rats. As increased brain 5-HT turnover was observed in raph\u00e9-lesioned rats, this may have prevented a decrease in blood 5-HIAA-14C levels. The results suggest that changes in blood 5-HIAA-14C following MK-486 and 5-HTP-14C may be related to changes in brain 5-HT-14C metabolism.", "contents": "Effect of midbrain raph\u00e9 lesions or intracisternal 6-hydroxydopamine administration on D, L-5-hydroxytryptophan-14C metabolism after peripheral decarboxylase inhibition. Brain and blood 14C-5-hydroxyindole metabolism was studied in normal, raph\u00e9-lesioned and 6-hydroxydopamine (60HDA)-treated rats given L-(-)-alpha-hydrazino-3, 4-dihydroxy-alpha-methylhydrocinnamic acid (MK-486) (50 mg/kg) intraperitoneally and 5-hydroxytryptophan (5-HTP)-14C (5 muCi, 0.51 mCi/mmol) intravenously. Raph\u00e9 lesioning and 60HDA treatment caused significant decrements in brain 5-hydroxytryptamine (5-HT)-14C and 5-hydroxyindoleacetic acid (5-HIAA)-14C levels and 5-HT concentrations compared to those in normal controls. In normal and 60HDA-treated rats there were decreased brain dopamine levels, possibly a consequence of non-specific metabolism of 5-HTP in brain. Blood 5-HIAA-14C levels were significantly decreased in 60HDA-treated but not raph\u00e9-lesioned rats. As increased brain 5-HT turnover was observed in raph\u00e9-lesioned rats, this may have prevented a decrease in blood 5-HIAA-14C levels. The results suggest that changes in blood 5-HIAA-14C following MK-486 and 5-HTP-14C may be related to changes in brain 5-HT-14C metabolism."}
{"id": "PMID:1086140", "title": "The ontogenesis of monoaminergic nerve fibres in the hypophysis of Rana temporaria with special reference to the pars distalis.", "content": "In Rana temporaria tadpoles, fluorescent fibres appear in the prospective eminentia mediana, the pars intermedia and the pars distalis at Gosner's stage 25. During prometamorphosis the amount of fluorescent material increases around the developing primary capillary plexus in the eminentia mediana. In the pars intermedia the fibres form a dense fluorescent network but in the pars distalis the fibres are few and delicate. At stages 42-43, the onset of climax, the pars distalis fibres disappear. The possible functional significance of the pars distalis fibres is discussed. The background adaptation ability appears at stages 28-29, while the fluorescent pars intermedia innervation is observable at stage 25.", "contents": "The ontogenesis of monoaminergic nerve fibres in the hypophysis of Rana temporaria with special reference to the pars distalis. In Rana temporaria tadpoles, fluorescent fibres appear in the prospective eminentia mediana, the pars intermedia and the pars distalis at Gosner's stage 25. During prometamorphosis the amount of fluorescent material increases around the developing primary capillary plexus in the eminentia mediana. In the pars intermedia the fibres form a dense fluorescent network but in the pars distalis the fibres are few and delicate. At stages 42-43, the onset of climax, the pars distalis fibres disappear. The possible functional significance of the pars distalis fibres is discussed. The background adaptation ability appears at stages 28-29, while the fluorescent pars intermedia innervation is observable at stage 25."}
{"id": "PMID:1086147", "title": "Tumours of the upper alimentary tract.", "content": "Tumours of the oropharynx of domestic animals are common in most parts of the world, but squamous cell carcinoma of the upper alimentary tract shows differences in prevalence in different geographical areas and occurs at different sites in the various species. Oral tumours of the melanogenic system are more common in dogs than in man. The following main histological categories, which broadly correspond to those used in the classification of tumours of man, are described: papilloma; squamous cell carcinoma; salivary gland tumours; malignant melanoma; tumours of soft (mesenchymal) tissues; tumours of the facial bones; tumours of haematopoietic and related tissues; and odontogenic tumours and jaw cysts. Papilloma, squamous cell carcinoma, malignant melanoma, fibroma, and fibrosarcoma account for about 80% of the tumours that occur in the upper alimentary tract of domestic animals.", "contents": "Tumours of the upper alimentary tract. Tumours of the oropharynx of domestic animals are common in most parts of the world, but squamous cell carcinoma of the upper alimentary tract shows differences in prevalence in different geographical areas and occurs at different sites in the various species. Oral tumours of the melanogenic system are more common in dogs than in man. The following main histological categories, which broadly correspond to those used in the classification of tumours of man, are described: papilloma; squamous cell carcinoma; salivary gland tumours; malignant melanoma; tumours of soft (mesenchymal) tissues; tumours of the facial bones; tumours of haematopoietic and related tissues; and odontogenic tumours and jaw cysts. Papilloma, squamous cell carcinoma, malignant melanoma, fibroma, and fibrosarcoma account for about 80% of the tumours that occur in the upper alimentary tract of domestic animals."}
{"id": "PMID:1086148", "title": "Tumours of the lower alimentary tract.", "content": "THIS CLASSIFICATION IS PRESENTED IN TWO PARTS: (a) tumours of the gastrointestinal tract; and (b) tumours of the anal canal and margin. In the gastrointestinal tract the tumours are classified as adenoma, adenocarcinoma, and undifferentiated carcinoma, with several subtypes. Most polyps prove to be non-neoplastic, hyperplastic, or regenerative rather than adenomatous. Carcinoma of the stomach occurs mainly in dogs, but is a rare tumour in all parts of the world. Moderately differentiated, tubular adenocarcinoma of the small intestine with excessive fibrosis occurs in all six species; in some geographical locations it may occur frequently in sheep and cattle. The adenoma/carcinoma sequence in the rectum of the dog is similar to that in man but is encountered less often. Carcinoid tumours are very rare in domestic animals. Among the soft tissue tumours, those of smooth muscle and adipose tissue are found fairly frequently and congenital mesothelioma in the peritoneum of calves occurs occasionally. Tumours of the haematopoietic and related tissues are the most common gastrointestinal neoplasms in all species and most belong to the lymphosarcoma group. Tumours of the anal canal and margin are common in the dog and 90% of these are tumours of the hepatoid (perianal) glands.", "contents": "Tumours of the lower alimentary tract. THIS CLASSIFICATION IS PRESENTED IN TWO PARTS: (a) tumours of the gastrointestinal tract; and (b) tumours of the anal canal and margin. In the gastrointestinal tract the tumours are classified as adenoma, adenocarcinoma, and undifferentiated carcinoma, with several subtypes. Most polyps prove to be non-neoplastic, hyperplastic, or regenerative rather than adenomatous. Carcinoma of the stomach occurs mainly in dogs, but is a rare tumour in all parts of the world. Moderately differentiated, tubular adenocarcinoma of the small intestine with excessive fibrosis occurs in all six species; in some geographical locations it may occur frequently in sheep and cattle. The adenoma/carcinoma sequence in the rectum of the dog is similar to that in man but is encountered less often. Carcinoid tumours are very rare in domestic animals. Among the soft tissue tumours, those of smooth muscle and adipose tissue are found fairly frequently and congenital mesothelioma in the peritoneum of calves occurs occasionally. Tumours of the haematopoietic and related tissues are the most common gastrointestinal neoplasms in all species and most belong to the lymphosarcoma group. Tumours of the anal canal and margin are common in the dog and 90% of these are tumours of the hepatoid (perianal) glands."}
{"id": "PMID:1086149", "title": "Tumours of the liver and biliary system.", "content": "In this histological classification of liver and gall bladder tumours the tumour types largely correspond to those found in man. The most common tumours in this group are liver cell adenoma, hepatocellular carcinoma, and cholangiocarcinoma.", "contents": "Tumours of the liver and biliary system. In this histological classification of liver and gall bladder tumours the tumour types largely correspond to those found in man. The most common tumours in this group are liver cell adenoma, hepatocellular carcinoma, and cholangiocarcinoma."}
{"id": "PMID:1086150", "title": "Tumours of the pancreas.", "content": "Tumours of the pancreas occur most commonly in dogs and cats and only rarely in other domestic species. The incidence of neoplasms, both exocrine and endocrine, increases with age. Exocrine adenocarcinomas are the most common malignant tumours and have three fairly distinct morphological patterns: small tubular, large tubular, and acinar cell (rare). They readily metastasize, usually before clinical signs are apparent. A \"starry sky\" pattern with clear histiocytes scattered among tumour cells is a regular feature of poorly differentiated areas of small tubular adenocarcinomas and undifferentiated carcinomas. Islet cell tumours occur in a significant number only in dogs. Metastases are found in about half of the tumours, but malignancy cannot always be predicted by the morphological appearance. Slightly more than half of the islet cell tumours reported in the dog have been associated with clinical signs of hypoglycaemia. Nodular hyperplasia and exocrine adenomas are sometimes difficult to differentiate. Adenomas are considered rare while nodular hyperplasia is common in old animals.", "contents": "Tumours of the pancreas. Tumours of the pancreas occur most commonly in dogs and cats and only rarely in other domestic species. The incidence of neoplasms, both exocrine and endocrine, increases with age. Exocrine adenocarcinomas are the most common malignant tumours and have three fairly distinct morphological patterns: small tubular, large tubular, and acinar cell (rare). They readily metastasize, usually before clinical signs are apparent. A \"starry sky\" pattern with clear histiocytes scattered among tumour cells is a regular feature of poorly differentiated areas of small tubular adenocarcinomas and undifferentiated carcinomas. Islet cell tumours occur in a significant number only in dogs. Metastases are found in about half of the tumours, but malignancy cannot always be predicted by the morphological appearance. Slightly more than half of the islet cell tumours reported in the dog have been associated with clinical signs of hypoglycaemia. Nodular hyperplasia and exocrine adenomas are sometimes difficult to differentiate. Adenomas are considered rare while nodular hyperplasia is common in old animals."}
{"id": "PMID:1086151", "title": "Tumours of the ovary.", "content": "Ovarian tumours are common in animals, the majority occurring in bitches and cows. The two most important germ cell tumours were dysgerminoma and teratoma; these morphologically resemble their counterparts in women, with the exception that teratomas in animals tend less to malignancy. The granulosa cell tumour is the most frequent sex cord-stromal tumour in all six species and it may contain luteinized areas or show differentiation towards a Sertoli cell pattern. The canine papillary adenoma and papillary adenocarcinoma, which are as common as granulosa tumours, have several features in common with their counterparts in women: they are of similar histological appearance, are frequently bilateral, and the adenocarcinomas have a great propensity for peritoneal implantation metastasis. Ovarian cysts are frequent in the bitch, sow, and cow and may originate from five different anatomical structures in the ovary.", "contents": "Tumours of the ovary. Ovarian tumours are common in animals, the majority occurring in bitches and cows. The two most important germ cell tumours were dysgerminoma and teratoma; these morphologically resemble their counterparts in women, with the exception that teratomas in animals tend less to malignancy. The granulosa cell tumour is the most frequent sex cord-stromal tumour in all six species and it may contain luteinized areas or show differentiation towards a Sertoli cell pattern. The canine papillary adenoma and papillary adenocarcinoma, which are as common as granulosa tumours, have several features in common with their counterparts in women: they are of similar histological appearance, are frequently bilateral, and the adenocarcinomas have a great propensity for peritoneal implantation metastasis. Ovarian cysts are frequent in the bitch, sow, and cow and may originate from five different anatomical structures in the ovary."}
{"id": "PMID:1086152", "title": "Tumours of the female genital tract.", "content": "Tumours of the female tubular genital tract are comparatively rare, with the exception of leiomyomas in cows and bitches, uterine carcinomas and vaginal fibropapillomas in cows, and transmissible venereal tumours in bitches. Uterine adenocarcinomas of cows are highly scirrhous, often causing minimal gross lesions that remain undetected until metastatic lesions in pelvic nodes and lungs are found. Cats and bitches also develop uterine carcinomas, but less frequently than cows; when present, they are predominantly discrete masses of well differentiated, non-sclerosing adenocarcinoma. Fibropapillomas are caused by the virus of verrucca vulgaris and can be transmitted to the penis of the bull. Adenomyosis is not uncommon in the cat, cow, and bitch. There is a marked difference in the frequency with which cervical carcinomas occur in man compared with other mammals; in the latter we could find no instance of an unequivocal primary cervical carcinoma. There are a few reports describing invasive carcinomas involving the cervix, but invasion from either a uterine or a vaginal carcinoma could not be ruled out.", "contents": "Tumours of the female genital tract. Tumours of the female tubular genital tract are comparatively rare, with the exception of leiomyomas in cows and bitches, uterine carcinomas and vaginal fibropapillomas in cows, and transmissible venereal tumours in bitches. Uterine adenocarcinomas of cows are highly scirrhous, often causing minimal gross lesions that remain undetected until metastatic lesions in pelvic nodes and lungs are found. Cats and bitches also develop uterine carcinomas, but less frequently than cows; when present, they are predominantly discrete masses of well differentiated, non-sclerosing adenocarcinoma. Fibropapillomas are caused by the virus of verrucca vulgaris and can be transmitted to the penis of the bull. Adenomyosis is not uncommon in the cat, cow, and bitch. There is a marked difference in the frequency with which cervical carcinomas occur in man compared with other mammals; in the latter we could find no instance of an unequivocal primary cervical carcinoma. There are a few reports describing invasive carcinomas involving the cervix, but invasion from either a uterine or a vaginal carcinoma could not be ruled out."}
{"id": "PMID:1086153", "title": "Tumours of the adrenal gland and paraganglia.", "content": "This classification is arranged in two parts in order to take into account the different origins, structures, and functions of the cortex and medulla. The tabular classification is a simplified version of that suggested for adrenal tumours in man, and includes cortical adenoma and carcinoma, phaeochromocytoma, chemodectoma, neurofibroma, ganglioneuroma and ganglioneuroblastoma, and neuroblastoma. A detailed functional classification is not given, since the hormonal activity of many adrenal tumours in animals is less well known than it is in man. Of the tumour-like lesions listed, cortical hyperplasia is particularly important in several species.", "contents": "Tumours of the adrenal gland and paraganglia. This classification is arranged in two parts in order to take into account the different origins, structures, and functions of the cortex and medulla. The tabular classification is a simplified version of that suggested for adrenal tumours in man, and includes cortical adenoma and carcinoma, phaeochromocytoma, chemodectoma, neurofibroma, ganglioneuroma and ganglioneuroblastoma, and neuroblastoma. A detailed functional classification is not given, since the hormonal activity of many adrenal tumours in animals is less well known than it is in man. Of the tumour-like lesions listed, cortical hyperplasia is particularly important in several species."}
{"id": "PMID:1086154", "title": "Tumors of the kidney.", "content": "The most frequent renal tumours of animals are renal cell carcinoma and nephroblastoma. Renal cell carcinomas are seen mainly in dogs and cattle and nephroblastoma is encountered in pigs, puppies, and calves. Renal cell carcinomas are usually papillary in the dog. They show a marked propensity for vascular invasion, penetration of the posterior vena cava, and subsequent pulmonary metastasis. Nephroblastoma, which is morphologically identical to Wilms' tumour of children, is almost always a benign tumour in animals. It is one of the most frequent neoplasms of pigs, possibly owing to the fact that most pigs are slaughtered (and examined) when a few months old. Lymphosarcoma involving the kidney is particularly frequent in the cat, but is also seen in other species as part of a generalized disease.", "contents": "Tumors of the kidney. The most frequent renal tumours of animals are renal cell carcinoma and nephroblastoma. Renal cell carcinomas are seen mainly in dogs and cattle and nephroblastoma is encountered in pigs, puppies, and calves. Renal cell carcinomas are usually papillary in the dog. They show a marked propensity for vascular invasion, penetration of the posterior vena cava, and subsequent pulmonary metastasis. Nephroblastoma, which is morphologically identical to Wilms' tumour of children, is almost always a benign tumour in animals. It is one of the most frequent neoplasms of pigs, possibly owing to the fact that most pigs are slaughtered (and examined) when a few months old. Lymphosarcoma involving the kidney is particularly frequent in the cat, but is also seen in other species as part of a generalized disease."}
{"id": "PMID:1086155", "title": "Tumours of the prostate and penis.", "content": "Tumours of the male genital tract, excluding the testes, are relatively rare in the six major domestic animals. The most important tumours are prostate carcinoma and transmissible venereal tumour of the penis in dogs, fibropapilloma of the penis in bulls, squamous papilloma and squamous cell carcinoma in horses, and squamous papilloma in pigs. Four histological types of canine prostate carcinoma exist: alveolar papillary, acinar, organoid, and poorly differentiated. The biological behaviour of prostate carcinomas is similar to that in man, with frequent metastasis to the regional pelvic nodes, bones, and lungs. There appears to be no relationship between the common diffuse glandular hyperplasia and carcinoma in the prostate of dogs. A unique lesion of dogs is squamous metaplasia of the prostate related to estrogen-producing Sertoli cell tumours of the testis. Three different transmissible tumours of the penis occur in domestic animals. The canine venereal tumours can be transmitted only by intact tumour cells during licking and coital contact, whereas bovine fibropapillomas and porcine squamous papillomas can be transmitted by cell-free material. In cattle, the fibropapillomas are caused by the same virus that produces cutaneous papillomatosis. All three tumours are benign and usually regress spontaneously.", "contents": "Tumours of the prostate and penis. Tumours of the male genital tract, excluding the testes, are relatively rare in the six major domestic animals. The most important tumours are prostate carcinoma and transmissible venereal tumour of the penis in dogs, fibropapilloma of the penis in bulls, squamous papilloma and squamous cell carcinoma in horses, and squamous papilloma in pigs. Four histological types of canine prostate carcinoma exist: alveolar papillary, acinar, organoid, and poorly differentiated. The biological behaviour of prostate carcinomas is similar to that in man, with frequent metastasis to the regional pelvic nodes, bones, and lungs. There appears to be no relationship between the common diffuse glandular hyperplasia and carcinoma in the prostate of dogs. A unique lesion of dogs is squamous metaplasia of the prostate related to estrogen-producing Sertoli cell tumours of the testis. Three different transmissible tumours of the penis occur in domestic animals. The canine venereal tumours can be transmitted only by intact tumour cells during licking and coital contact, whereas bovine fibropapillomas and porcine squamous papillomas can be transmitted by cell-free material. In cattle, the fibropapillomas are caused by the same virus that produces cutaneous papillomatosis. All three tumours are benign and usually regress spontaneously."}
{"id": "PMID:1086156", "title": "Tumours of the nasal cavity.", "content": "Tumours of the nasal cavity are rare in domestic animals, most cases occurring in the dog. Epithelial tumours are the most common type in carnivores (dogs and cats). In general, the same types of tumour occur in domestic animals as occur in man. There was no significant predisposition for breed in dogs, but in both dogs and cats far more males than females were affected. Metastases occurred only rarely.", "contents": "Tumours of the nasal cavity. Tumours of the nasal cavity are rare in domestic animals, most cases occurring in the dog. Epithelial tumours are the most common type in carnivores (dogs and cats). In general, the same types of tumour occur in domestic animals as occur in man. There was no significant predisposition for breed in dogs, but in both dogs and cats far more males than females were affected. Metastases occurred only rarely."}
{"id": "PMID:1086157", "title": "Tumours of bones and joints.", "content": "Tumours of bones and joints are not infrequent in dogs but are rare in other domestic animals. In the dog, most bone tumours are malignant; osteosarcomas are by far the most frequently encountered tumours, especially in giant breeds and boxers. The following main categories of bone tumour are described: bone-forming, cartilage-forming, giant cell, marrow, vascular, miscellaneous, metastatic, unclassified, and tumour-like lesions. The tumours of joints and related structures are classified as synovial sarcomas, fibroxanthomas, and malignant giant cell tumour of soft tissues.", "contents": "Tumours of bones and joints. Tumours of bones and joints are not infrequent in dogs but are rare in other domestic animals. In the dog, most bone tumours are malignant; osteosarcomas are by far the most frequently encountered tumours, especially in giant breeds and boxers. The following main categories of bone tumour are described: bone-forming, cartilage-forming, giant cell, marrow, vascular, miscellaneous, metastatic, unclassified, and tumour-like lesions. The tumours of joints and related structures are classified as synovial sarcomas, fibroxanthomas, and malignant giant cell tumour of soft tissues."}
{"id": "PMID:1086158", "title": "Mercury in hair as an indicator of total body burden.", "content": "Hair is known to concentrate mercury, and in general the concentration of mercury in hair is proportional to and many times higher than its concentration in the blood. The variation of the mercury concentration in human head hair was used to follow the history of poisoning in people who ingested grain treated with methylmercury. Instrumental neutron activation analysis was used for mercury determination. The initial rising slope of mercury concentration along the hair was proportional to the daily intake of methylmercury per kg of body weight. The ratio of the concentration of methylmercury in human head hair to the average body concentration was found to be about 137. By using this ratio and measuring mercury concentration in hair, the total body burden can be calculated. In the patients studied, the peak body burden ranged from 0.8 to 4.4 mg/kg in cases showing mild symptoms, from 1.5 to 6 mg/kg in cases with moderate symptoms, and from 3 to 12 mg/kg in cases with severe symptoms. The curve of the variation in mercury concentrations along the hair was also used to calculate the biological half-life of methylmercury in man. Forty-eight cases were studied and it was found that the frequency curve (population distribution curve) was grouped into two distinct regions. In about 90% of the population the biological half-life of methylmercury was 35-100 days, and 10% showed high values of 110-120 days.", "contents": "Mercury in hair as an indicator of total body burden. Hair is known to concentrate mercury, and in general the concentration of mercury in hair is proportional to and many times higher than its concentration in the blood. The variation of the mercury concentration in human head hair was used to follow the history of poisoning in people who ingested grain treated with methylmercury. Instrumental neutron activation analysis was used for mercury determination. The initial rising slope of mercury concentration along the hair was proportional to the daily intake of methylmercury per kg of body weight. The ratio of the concentration of methylmercury in human head hair to the average body concentration was found to be about 137. By using this ratio and measuring mercury concentration in hair, the total body burden can be calculated. In the patients studied, the peak body burden ranged from 0.8 to 4.4 mg/kg in cases showing mild symptoms, from 1.5 to 6 mg/kg in cases with moderate symptoms, and from 3 to 12 mg/kg in cases with severe symptoms. The curve of the variation in mercury concentrations along the hair was also used to calculate the biological half-life of methylmercury in man. Forty-eight cases were studied and it was found that the frequency curve (population distribution curve) was grouped into two distinct regions. In about 90% of the population the biological half-life of methylmercury was 35-100 days, and 10% showed high values of 110-120 days."}
{"id": "PMID:1086159", "title": "Environmental contamination by mercury in Iraq.", "content": "Following the outbreak of organomercury poisoning in Iraq, an investigation was carried out during the spring and summer of 1972 to evaluate environmental contamination by organomercury.Analyses were performed on fish of several species (not typical predators) and on a few specimens of aquatic birds (not fish-eating). Most fish samples contained concentrations in muscle ranging from 0.01 to 0.15 mg/kg. These concentrations are within the range found in tropical rivers. Higher figures, 0.3-0.5 mg/kg, were found downstream from a caustic soda plant south of Baghdad. A few cases of very high mercury concentrations (25-30 mg/kg) were reported from an area where fish kills had occurred. Aquatic birds (ducks and waders) contained low concentrations of mercury (900-2750 ng/g). Tail feathers of seed-eating birds were found to contain 13 500-21 000 ng/g of mercury, which is about 10 times higher than values reported from Ethiopia and within the range found in Sweden and Canada. Insect-eating birds contained 1850-5200 ng/g, which is thought to be slightly elevated. Extremely high concentrations of mercury were found in muscle tissue of dead seed-eating birds (15 000-40 000 ng/g), while feathers contained similar concentrations (9000-52 000 ng/g). These extremely high concentrations were found only in the vicinity of storehouses where treated seed was kept. No birds of prey could be caught and analysed.", "contents": "Environmental contamination by mercury in Iraq. Following the outbreak of organomercury poisoning in Iraq, an investigation was carried out during the spring and summer of 1972 to evaluate environmental contamination by organomercury.Analyses were performed on fish of several species (not typical predators) and on a few specimens of aquatic birds (not fish-eating). Most fish samples contained concentrations in muscle ranging from 0.01 to 0.15 mg/kg. These concentrations are within the range found in tropical rivers. Higher figures, 0.3-0.5 mg/kg, were found downstream from a caustic soda plant south of Baghdad. A few cases of very high mercury concentrations (25-30 mg/kg) were reported from an area where fish kills had occurred. Aquatic birds (ducks and waders) contained low concentrations of mercury (900-2750 ng/g). Tail feathers of seed-eating birds were found to contain 13 500-21 000 ng/g of mercury, which is about 10 times higher than values reported from Ethiopia and within the range found in Sweden and Canada. Insect-eating birds contained 1850-5200 ng/g, which is thought to be slightly elevated. Extremely high concentrations of mercury were found in muscle tissue of dead seed-eating birds (15 000-40 000 ng/g), while feathers contained similar concentrations (9000-52 000 ng/g). These extremely high concentrations were found only in the vicinity of storehouses where treated seed was kept. No birds of prey could be caught and analysed."}
{"id": "PMID:1086160", "title": "Experimental studies on organomercury poisoning in chickens in Iraq.", "content": "For 164 days mature chickens received a daily diet containing 40 g of wheat treated with organomercurial fungicide and 80 g of untreated diet. A control group received 120 g of normal diet daily. The experimental group was then split - half receiving the same diet for a further 155 days, by which time all had been killed or had died, the other half being fed the same diet as the controls. No cases of mercurialism were seen in the experimental group, although 2 died after 143 and 319 days respectively. Egg production fluctuated as between the groups. Mercury levels in egg albumin showed high peaks after 98 and 108 days. After the feeding of contaminated wheat had been discontinued the albumin mercury level did not fall consistently below 0.5 mg/kg for 127 days. Levels in egg yolk always remained below the level in wheat and dropped to zero 49 days after feeding of the dressed wheat had been discontinued. Mercury levels found in tissue were similar to those described by other authors. Levels in the edible parts of the single cock examined exceeded those in the hens. Implications of these findings for public health decisions on the use of chicken and eggs as food when the birds have had access to contaminated grain are discussed.", "contents": "Experimental studies on organomercury poisoning in chickens in Iraq. For 164 days mature chickens received a daily diet containing 40 g of wheat treated with organomercurial fungicide and 80 g of untreated diet. A control group received 120 g of normal diet daily. The experimental group was then split - half receiving the same diet for a further 155 days, by which time all had been killed or had died, the other half being fed the same diet as the controls. No cases of mercurialism were seen in the experimental group, although 2 died after 143 and 319 days respectively. Egg production fluctuated as between the groups. Mercury levels in egg albumin showed high peaks after 98 and 108 days. After the feeding of contaminated wheat had been discontinued the albumin mercury level did not fall consistently below 0.5 mg/kg for 127 days. Levels in egg yolk always remained below the level in wheat and dropped to zero 49 days after feeding of the dressed wheat had been discontinued. Mercury levels found in tissue were similar to those described by other authors. Levels in the edible parts of the single cock examined exceeded those in the hens. Implications of these findings for public health decisions on the use of chicken and eggs as food when the birds have had access to contaminated grain are discussed."}
{"id": "PMID:1086161", "title": "Indirect sources of mercury poisoning in the Iraqi epidemic.", "content": "Secondary sources of intoxication with methylmercury poisoning other than home-made bread were explored. Mercury levels in various food items were below 0.05 mg/kg.Over 30 000 specimens of meat and internal organs of sheep were analysed because of the strong suspicion that the animals had been fed treated barley. It was found that 6-12% of the specimens analysed contained more than 0.5 mg/kg of mercury. Consumption of meat did not appear to constitute a danger of intoxication during the outbreak.", "contents": "Indirect sources of mercury poisoning in the Iraqi epidemic. Secondary sources of intoxication with methylmercury poisoning other than home-made bread were explored. Mercury levels in various food items were below 0.05 mg/kg.Over 30 000 specimens of meat and internal organs of sheep were analysed because of the strong suspicion that the animals had been fed treated barley. It was found that 6-12% of the specimens analysed contained more than 0.5 mg/kg of mercury. Consumption of meat did not appear to constitute a danger of intoxication during the outbreak."}
{"id": "PMID:1086162", "title": "Misuse of organomercury fungicides in Brazil.", "content": "A methoxyethyl mercuric compound, licensed in Brazil as a fungicide for seed dressing, was widely used for spraying tomato and other vegetable crops in 1966 and 1967.Mercury residues ranging from 0.05 to 0.30 mg/kg were detected in 14% of the tomatoes, 13% of other vegetable samples, and 57% of the tomato paste batches.A wide campaign through newspapers, radio, and television was quickly established to educate farmers and the population in general on the hazards of ingestion of mercury-contaminated food.All samples of tomatoes and other vegetables positive for mercury (residues above 0.05 mg/kg) were destroyed. Inspection posts on the main roads and highways prevented the delivery of contaminated food to cities and food processing plants.The sale of organomercury fungicides is now controlled in Brazil; however, treated seeds which are not planted may be diverted to human or animal consumption. There is no official record of accidents, but occasional cases of poisoning are known.The mercury residue level in Brazilian fish is low, indicating that environmental pollution by mercury is not a big problem in the country. However, some fresh-water and estuarine fish may contain mercury residues ranging from 0.01 to 0.66 mg/kg.", "contents": "Misuse of organomercury fungicides in Brazil. A methoxyethyl mercuric compound, licensed in Brazil as a fungicide for seed dressing, was widely used for spraying tomato and other vegetable crops in 1966 and 1967.Mercury residues ranging from 0.05 to 0.30 mg/kg were detected in 14% of the tomatoes, 13% of other vegetable samples, and 57% of the tomato paste batches.A wide campaign through newspapers, radio, and television was quickly established to educate farmers and the population in general on the hazards of ingestion of mercury-contaminated food.All samples of tomatoes and other vegetables positive for mercury (residues above 0.05 mg/kg) were destroyed. Inspection posts on the main roads and highways prevented the delivery of contaminated food to cities and food processing plants.The sale of organomercury fungicides is now controlled in Brazil; however, treated seeds which are not planted may be diverted to human or animal consumption. There is no official record of accidents, but occasional cases of poisoning are known.The mercury residue level in Brazilian fish is low, indicating that environmental pollution by mercury is not a big problem in the country. However, some fresh-water and estuarine fish may contain mercury residues ranging from 0.01 to 0.66 mg/kg."}
{"id": "PMID:1086163", "title": "An outbreak of organomercury poisoning among Iraqi farmers.", "content": "An outbreak of organomercury poisoning due to the consumption of treated grain by farmers and their families occurred in Iraq in 1971-72. A total of 6530 cases were admitted to hospital and of these 459 died. However, there were many more with minor symptoms of poisoning who consulted outpatient departments. This outbreak constituted the largest poisoning epidemic ever recorded. No age was exempt and no pronounced sex difference was apparent. The latent period of up to 60 days between dosage and the onset of symptoms was probably the major factor contributing to the size of the epidemic. Measures taken to limit the outbreak are outlined.", "contents": "An outbreak of organomercury poisoning among Iraqi farmers. An outbreak of organomercury poisoning due to the consumption of treated grain by farmers and their families occurred in Iraq in 1971-72. A total of 6530 cases were admitted to hospital and of these 459 died. However, there were many more with minor symptoms of poisoning who consulted outpatient departments. This outbreak constituted the largest poisoning epidemic ever recorded. No age was exempt and no pronounced sex difference was apparent. The latent period of up to 60 days between dosage and the onset of symptoms was probably the major factor contributing to the size of the epidemic. Measures taken to limit the outbreak are outlined."}
{"id": "PMID:1086164", "title": "Epidemiology of organomercury poisoning in Iraq. I. Incidence in a defined area and relationship to the eating of contaminated bread.", "content": "A survey was carried out in a defined area in rural Iraq where there had been many cases of organomercury poisoning following the consumption of bread contaminated by mercury, in order to determine the true incidence of the disorder. The results were compared with those obtained from a similar rural area from which few cases had been reported. A questionnaire was used to determine the amount of contaminated bread eaten and the frequency of symptoms; a simple clinical examination was performed and blood and hair samples were collected for estimation of total mercury concentration. Of 700 people over the age of 5 years in the high-exposure area, 66% admitted to having eaten contaminated bread, while none of the 864 persons in the low exposure area had done so. The mean period during which contaminated bread was eaten was 32 days, but some people had eaten it for as long as 3 months. A mean of 121 loaves was eaten, the maximum being 480 loaves. For the mean number of loaves the intake of methylmercury was likely to have been between 80 mg and 250 mg, but the people who had consumed the largest amount of contaminated bread may have ingested up to 1 000 mg of methylmercury over a 3-month period. Of those with signs of alkylmercury poisoning at the time of the survey, 80% had eaten more than 100 loaves, and 53 (71%) out of 75 persons who had eaten more than 200 loaves showed some evidence of poisoning.The incidence rate for poisoning was estimated at 271 per 1 000; this figure includes a mortality rate of 59 per 1 000, 32 per 1 000 cases with severe disability, 41 per 1 000 cases with mild or moderate disability and 138 per 1 000 cases with only subjective evidence of poisoning at the time of the study.", "contents": "Epidemiology of organomercury poisoning in Iraq. I. Incidence in a defined area and relationship to the eating of contaminated bread. A survey was carried out in a defined area in rural Iraq where there had been many cases of organomercury poisoning following the consumption of bread contaminated by mercury, in order to determine the true incidence of the disorder. The results were compared with those obtained from a similar rural area from which few cases had been reported. A questionnaire was used to determine the amount of contaminated bread eaten and the frequency of symptoms; a simple clinical examination was performed and blood and hair samples were collected for estimation of total mercury concentration. Of 700 people over the age of 5 years in the high-exposure area, 66% admitted to having eaten contaminated bread, while none of the 864 persons in the low exposure area had done so. The mean period during which contaminated bread was eaten was 32 days, but some people had eaten it for as long as 3 months. A mean of 121 loaves was eaten, the maximum being 480 loaves. For the mean number of loaves the intake of methylmercury was likely to have been between 80 mg and 250 mg, but the people who had consumed the largest amount of contaminated bread may have ingested up to 1 000 mg of methylmercury over a 3-month period. Of those with signs of alkylmercury poisoning at the time of the survey, 80% had eaten more than 100 loaves, and 53 (71%) out of 75 persons who had eaten more than 200 loaves showed some evidence of poisoning.The incidence rate for poisoning was estimated at 271 per 1 000; this figure includes a mortality rate of 59 per 1 000, 32 per 1 000 cases with severe disability, 41 per 1 000 cases with mild or moderate disability and 138 per 1 000 cases with only subjective evidence of poisoning at the time of the study."}
{"id": "PMID:1086165", "title": "Epidemiology of organomercury poisoning in Iraq. II. Relationship of mercury levels in blood and hair to exposure and to clinical findings.", "content": "In the survey described by Al-Mufti et al. (see page 23) blood and hair samples were analysed for total mercury by modified atomic absorption spectrophotometry. The hair samples were divided into 2.5-cm segments and analysed consecutively. The mean blood levels were 34 ng/ml and 7 ng/ml, respectively in those who had and those who had not eaten contaminated bread.Corresponding mean maximum hair mercury values were 136 mug/g and 5 mug/g, respectively. Hair mercury values provided a better discrimination between different categories of exposure than blood mercury values at the time the survey was performed, some months after the end of the outbreak. Those persons who had not eaten contaminated bread but who lived in the area of high exposure had hair mercury values between the values of those who had eaten and those who had not eaten contaminated bread and who lived in the area of low exposure. Sequential estimation of mercury in 2.5-cm segments of hair in women gave information on the period of accumulation of mercury more than 1 year before the time of collection of the samples. It was possible to show an approximate relationship between the maximum hair mercury value and the amount of contaminated bread eaten. The match between the blood mercury level and the severity of poisoning was poor, owing to the length of time that had elapsed between the onset of poisoning and the sampling. With hair mercury, while the group results showed a good relation to the severity of poisoning, in individual cases the match was less good, especially in those persons where an insufficient length of hair was available for analysis. Biological variation in sensitivity to methylmercury was also likely to have been an important factor.", "contents": "Epidemiology of organomercury poisoning in Iraq. II. Relationship of mercury levels in blood and hair to exposure and to clinical findings. In the survey described by Al-Mufti et al. (see page 23) blood and hair samples were analysed for total mercury by modified atomic absorption spectrophotometry. The hair samples were divided into 2.5-cm segments and analysed consecutively. The mean blood levels were 34 ng/ml and 7 ng/ml, respectively in those who had and those who had not eaten contaminated bread.Corresponding mean maximum hair mercury values were 136 mug/g and 5 mug/g, respectively. Hair mercury values provided a better discrimination between different categories of exposure than blood mercury values at the time the survey was performed, some months after the end of the outbreak. Those persons who had not eaten contaminated bread but who lived in the area of high exposure had hair mercury values between the values of those who had eaten and those who had not eaten contaminated bread and who lived in the area of low exposure. Sequential estimation of mercury in 2.5-cm segments of hair in women gave information on the period of accumulation of mercury more than 1 year before the time of collection of the samples. It was possible to show an approximate relationship between the maximum hair mercury value and the amount of contaminated bread eaten. The match between the blood mercury level and the severity of poisoning was poor, owing to the length of time that had elapsed between the onset of poisoning and the sampling. With hair mercury, while the group results showed a good relation to the severity of poisoning, in individual cases the match was less good, especially in those persons where an insufficient length of hair was available for analysis. Biological variation in sensitivity to methylmercury was also likely to have been an important factor."}
{"id": "PMID:1086166", "title": "Epidemiology of organomercury poisoning in Iraq. III. Clinical features and their changes with time.", "content": "Three categories of mercury poisoning were defined in the survey described by Al-Mufti et al. (see page 23) and the age-specific incidence rates for these are given. Persons with physical signs consistent with a diagnosis of organomercury poisoning were allocated to categories of severe disability or mild/moderate disability. However, the largest category consisted of persons who had symptoms but no readily elicitable physical signs at the time of the survey. These symptoms followed a consistent pattern with paraesthesia involving the lips and/or circumoral region or trunk and difficulty with walking, described as weakness or unsteadiness of the legs, and in some cases repeated falls, forming the most commonly occurring symptom complex. Mean maximum hair mercury levels differentiated this group very clearly from the group with no symptoms of mercury poisoning. Very few people in the area of low exposure complained of such symptoms; where they did occur they were less well related to the time of the outbreak and showed little tendency to improve. Most people reported improvement in their symptoms by the time of the survey, with more improvement in some symptoms than in others. However, it is not known whether those people with symptoms only at the time of the survey had had at an earlier stage mild signs which had cleared. It was thought unlikely that further substantial improvement would occur in those persons with disability at the time of the survey.", "contents": "Epidemiology of organomercury poisoning in Iraq. III. Clinical features and their changes with time. Three categories of mercury poisoning were defined in the survey described by Al-Mufti et al. (see page 23) and the age-specific incidence rates for these are given. Persons with physical signs consistent with a diagnosis of organomercury poisoning were allocated to categories of severe disability or mild/moderate disability. However, the largest category consisted of persons who had symptoms but no readily elicitable physical signs at the time of the survey. These symptoms followed a consistent pattern with paraesthesia involving the lips and/or circumoral region or trunk and difficulty with walking, described as weakness or unsteadiness of the legs, and in some cases repeated falls, forming the most commonly occurring symptom complex. Mean maximum hair mercury levels differentiated this group very clearly from the group with no symptoms of mercury poisoning. Very few people in the area of low exposure complained of such symptoms; where they did occur they were less well related to the time of the outbreak and showed little tendency to improve. Most people reported improvement in their symptoms by the time of the survey, with more improvement in some symptoms than in others. However, it is not known whether those people with symptoms only at the time of the survey had had at an earlier stage mild signs which had cleared. It was thought unlikely that further substantial improvement would occur in those persons with disability at the time of the survey."}
{"id": "PMID:1086167", "title": "Chemical type of mercury in patients in the outbreak of organomercury poisoning in Iraq.", "content": "Gas chromatography was employed to identify the mercurial compound involved in the Iraq outbreak of organomercury poisoning. Only methylmercury was detected in human blood, hair, and tissues. The quantity of methylmercury present correlates well with the quantity of organic mercury determined by selective atomic absorption.", "contents": "Chemical type of mercury in patients in the outbreak of organomercury poisoning in Iraq. Gas chromatography was employed to identify the mercurial compound involved in the Iraq outbreak of organomercury poisoning. Only methylmercury was detected in human blood, hair, and tissues. The quantity of methylmercury present correlates well with the quantity of organic mercury determined by selective atomic absorption."}
{"id": "PMID:1086168", "title": "Intoxication due to alkylmercury-treated seed--1971-72 outbreak in Iraq: clinical aspects.", "content": "Sixty-six hospitalized patients suffering from chronic methylmercury poisoning were examined in Baghdad during 1972. The poisoning was attributed to consumption of home-made bread prepared from seed wheat treated with mercurial fungicide. The age incidence ranged between 4 and 70 years.Of the various clinical features encountered, neurological symptoms and signs were predominant and included muscular weakness, numbness, unsteady gait, paraesthesia, dysarthria, mental disturbances and, in severe cases, blindness, partial deafness, stupor, coma, and death. Involvement of the cardiovascular, urinary, gastrointestinal and haemopoietic systems, which was commonly encountered in ethylmercury poisoning in the 1960 outbreak in Iraq, was unusual.The severity of symptoms and signs was, broadly speaking, dose-dependent; high exposure led to severe clinical manifestations, but variations existed. Criteria, based on the clinical manifestations, were set for grading the severity of cases. The series included 2 asymptomatic cases, 20 mild, 20 moderate, 14 severe, and 10 very severe. In the latter group 5 patients died from failure of the central nervous system.The severely poisoned patients died irrespective of the medical treatment received. After 2 years of observation, most patients graded as mild or moderate cases lost their symptoms completely. Severe cases improved slowly, although ataxia, diminution of visual field and acuity and paraesthesia were still present. Thus, the previously accepted view that neurological signs were irreversible has been disproved.", "contents": "Intoxication due to alkylmercury-treated seed--1971-72 outbreak in Iraq: clinical aspects. Sixty-six hospitalized patients suffering from chronic methylmercury poisoning were examined in Baghdad during 1972. The poisoning was attributed to consumption of home-made bread prepared from seed wheat treated with mercurial fungicide. The age incidence ranged between 4 and 70 years.Of the various clinical features encountered, neurological symptoms and signs were predominant and included muscular weakness, numbness, unsteady gait, paraesthesia, dysarthria, mental disturbances and, in severe cases, blindness, partial deafness, stupor, coma, and death. Involvement of the cardiovascular, urinary, gastrointestinal and haemopoietic systems, which was commonly encountered in ethylmercury poisoning in the 1960 outbreak in Iraq, was unusual.The severity of symptoms and signs was, broadly speaking, dose-dependent; high exposure led to severe clinical manifestations, but variations existed. Criteria, based on the clinical manifestations, were set for grading the severity of cases. The series included 2 asymptomatic cases, 20 mild, 20 moderate, 14 severe, and 10 very severe. In the latter group 5 patients died from failure of the central nervous system.The severely poisoned patients died irrespective of the medical treatment received. After 2 years of observation, most patients graded as mild or moderate cases lost their symptoms completely. Severe cases improved slowly, although ataxia, diminution of visual field and acuity and paraesthesia were still present. Thus, the previously accepted view that neurological signs were irreversible has been disproved."}
{"id": "PMID:1086169", "title": "Ocular manifestations of mercury poisoning.", "content": "One hundred patients suffering from organomercury poisoning who were hospitalized in the Medical City, University of Baghdad, were examined ophthalmologically in the period between March and December 1972, and were reviewed again about 10 months later.The examination included clinical signs and symptoms, slit lamp examination of the anterior segment of the eyes, examination for visual field changes and, in the autopsied cases, analysis of the optic nerve and the occipital cortex. In addition, aqueous humour was aspirated in 10 patients and in 10 controls for the estimation of the mercury level.", "contents": "Ocular manifestations of mercury poisoning. One hundred patients suffering from organomercury poisoning who were hospitalized in the Medical City, University of Baghdad, were examined ophthalmologically in the period between March and December 1972, and were reviewed again about 10 months later.The examination included clinical signs and symptoms, slit lamp examination of the anterior segment of the eyes, examination for visual field changes and, in the autopsied cases, analysis of the optic nerve and the occipital cortex. In addition, aqueous humour was aspirated in 10 patients and in 10 controls for the estimation of the mercury level."}
{"id": "PMID:1086170", "title": "Clinical observations on treatment of alkylmercury poisoning in hospital patients.", "content": "Twenty-six patients suffering from methylmercury poisoning were treated by different therapeutic regimes. Seven received penicillamine or N-acetyl-d-L-penicillamine, 10 were treated with dimercaprol sulfonate and 9, who could not be treated, were given a placebo and were used as controls. Penicillamine, N-acetyl-d-L-penicillamine, and dimercaprol sulfonate reduced the blood level of mercury and increased its urinary excretion. No appreciable clinical improvement was noticed among the severe or very severe cases, while mild or moderate cases improved clinically irrespective of the treatment given.", "contents": "Clinical observations on treatment of alkylmercury poisoning in hospital patients. Twenty-six patients suffering from methylmercury poisoning were treated by different therapeutic regimes. Seven received penicillamine or N-acetyl-d-L-penicillamine, 10 were treated with dimercaprol sulfonate and 9, who could not be treated, were given a placebo and were used as controls. Penicillamine, N-acetyl-d-L-penicillamine, and dimercaprol sulfonate reduced the blood level of mercury and increased its urinary excretion. No appreciable clinical improvement was noticed among the severe or very severe cases, while mild or moderate cases improved clinically irrespective of the treatment given."}
{"id": "PMID:1086171", "title": "Tissue levels of mercury in autopsy specimens of liver and kidney.", "content": "Fifty-one autopsy specimens of liver were analysed for total mercury. Thirteen specimens contained less than 10 mg/kg of mercury, with a minimum value of 1.4 mg/kg, indicating that death in suspected cases was not always due to lethal exposure to methylmercury. The methylmercury concentration in 28 livers was 10-30 mg/kg. Limited additional estimations have shown that 71% of the liver mercury was organic and that the level of mercury in the liver of a 7-month-old fetus was only 25% of that in the liver of the mother. In a patient who died in hospital with a blood mercury level of 4.1 mug/ml, the liver contained 16.5 mg/kg of mercury.Differences between these results and those found in the outbreak of methylmercury poisoning in Japan are discussed. Any extrapolation of tissue mercury levels in relation to the toxic effects of methylmercury must take account of the intensity and duration of exposure.", "contents": "Tissue levels of mercury in autopsy specimens of liver and kidney. Fifty-one autopsy specimens of liver were analysed for total mercury. Thirteen specimens contained less than 10 mg/kg of mercury, with a minimum value of 1.4 mg/kg, indicating that death in suspected cases was not always due to lethal exposure to methylmercury. The methylmercury concentration in 28 livers was 10-30 mg/kg. Limited additional estimations have shown that 71% of the liver mercury was organic and that the level of mercury in the liver of a 7-month-old fetus was only 25% of that in the liver of the mother. In a patient who died in hospital with a blood mercury level of 4.1 mug/ml, the liver contained 16.5 mg/kg of mercury.Differences between these results and those found in the outbreak of methylmercury poisoning in Japan are discussed. Any extrapolation of tissue mercury levels in relation to the toxic effects of methylmercury must take account of the intensity and duration of exposure."}
{"id": "PMID:1086172", "title": "Levels of mercury and pathological changes in patients with organomercury poisoning.", "content": "Autopsies were carried out on 4 adults who died during the outbreak of mercury poisoning in Iraq and on 4 infants who were exposed to organomercury in utero. Mercury levels in tissues and in some body fluids were determined. The high levels of mercury in the central nervous system and the marked neuronal degeneration are noted.", "contents": "Levels of mercury and pathological changes in patients with organomercury poisoning. Autopsies were carried out on 4 adults who died during the outbreak of mercury poisoning in Iraq and on 4 infants who were exposed to organomercury in utero. Mercury levels in tissues and in some body fluids were determined. The high levels of mercury in the central nervous system and the marked neuronal degeneration are noted."}
{"id": "PMID:1086173", "title": "The determination of 5-hydroxytryptophan and its metabolites in plasma following administration to man.", "content": "The estimation of 5-hydroxytryptophan and its metabolites 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in plasma is described. The method is based on solvent extraction and the use of a liquid cation exchange reagent to separate 5-hydroxytryptamine and 5-hydroxytryptophan. The 5-hydroxyindoles are determined fluorimetrically following derivatisation with o-phthalaldehyde in strong acid. Data on the specificity and linearity of the method are presented. The time course of plasma 5-hydroxyindoles after oral administration of 5-hydroxytryptophan is studied in two subjects as an example of the application of the method and of its possible role in monitoring the plasma levels in therapy or in provocative tests.", "contents": "The determination of 5-hydroxytryptophan and its metabolites in plasma following administration to man. The estimation of 5-hydroxytryptophan and its metabolites 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in plasma is described. The method is based on solvent extraction and the use of a liquid cation exchange reagent to separate 5-hydroxytryptamine and 5-hydroxytryptophan. The 5-hydroxyindoles are determined fluorimetrically following derivatisation with o-phthalaldehyde in strong acid. Data on the specificity and linearity of the method are presented. The time course of plasma 5-hydroxyindoles after oral administration of 5-hydroxytryptophan is studied in two subjects as an example of the application of the method and of its possible role in monitoring the plasma levels in therapy or in provocative tests."}
{"id": "PMID:1086178", "title": "Experimental study on the electrical impedance of bone and the effect of direct current on the healing of fractures.", "content": "An experimental study of the electrical impedance of cortical bone and medullary cavity and on the effect of a D.C. or a periodically interrupted D.C. on the healing of fractures in rabbits, suggests that the bone behaves as a capacitance of about 20 muF in parallel with a high D.C. resistance. Also a 10 muA periodically interrupted D.C. current stimulates osteogenesis.", "contents": "Experimental study on the electrical impedance of bone and the effect of direct current on the healing of fractures. An experimental study of the electrical impedance of cortical bone and medullary cavity and on the effect of a D.C. or a periodically interrupted D.C. on the healing of fractures in rabbits, suggests that the bone behaves as a capacitance of about 20 muF in parallel with a high D.C. resistance. Also a 10 muA periodically interrupted D.C. current stimulates osteogenesis."}
{"id": "PMID:1086179", "title": "Osteogenic sarcoma: state of the art with high-dose methotrexate treatment.", "content": "The development and application of the VCR-MTX-CF regimens for the treatment of osteogenic sarcoma have changed the biological behavior of the tumor. Recent results strongly project major advances for the future. Although the major effect of chemotherapy resides in the eradication of micrometastases, its application for treatment of the primary tumor may also be considered. However, careful experimental design and follow-up periods for several years will be required to determine the optimum approaches. For example, it is possible that the interaction between weekly VCR-MTX-CF and radiation therapy may assume increasing importance. Thus, with the effective application of VRC-MTX-CF, the management of osteogenic sarcoma has evolved into a multidisciplinary approach and future advances will be based on the collective judgement of specialists from many fields.", "contents": "Osteogenic sarcoma: state of the art with high-dose methotrexate treatment. The development and application of the VCR-MTX-CF regimens for the treatment of osteogenic sarcoma have changed the biological behavior of the tumor. Recent results strongly project major advances for the future. Although the major effect of chemotherapy resides in the eradication of micrometastases, its application for treatment of the primary tumor may also be considered. However, careful experimental design and follow-up periods for several years will be required to determine the optimum approaches. For example, it is possible that the interaction between weekly VCR-MTX-CF and radiation therapy may assume increasing importance. Thus, with the effective application of VRC-MTX-CF, the management of osteogenic sarcoma has evolved into a multidisciplinary approach and future advances will be based on the collective judgement of specialists from many fields."}
{"id": "PMID:1086181", "title": "Ventricular size following head injury: a clinico-radiological study.", "content": "A retrospective study of the records of 93 patients who had sustained a severe closed head injury was carried out to determine the relationship, if any, between the severity of the injury, the size of the cerebral ventricles and the residual disability. Ventricular size was expressed as a ventricular index. The callosal angle was also measured to see whether this was of value in the recognition of post-traumatic external obstructive hydrocephalus. A normal range of ventricular indices and callosal angles was obtained from a review of the air encephalograms of 56 patients matched for age and sex with the head injury group, and whose air studies were considered to be normal. The findings on the head injury group were subjected to detailed statistical analysis. The study showed that there was no statistically significant relationship between severity of injury, measured by duration of unconsciousness, and ventricular size expressed as a ventricular index. There was a relationship between ventricular size and residual disability as graded in this study. If age was taken into consideration, the relationship between ventricular size and disability was closer. It was not possible to predict residual disability of individual patients from ventricular size alone due to the wide range of values in any disability grade. Failure of air to pass from the basal cisterns over the cerebral convexities is a widely accepted sign of extraventricular obstruction. The shapes of the ventricles, the ventricular index and a callosal angle of 110 degrees or less provided supporting evidence of obstruction in this study. Recognition of an obstructive element in ventricular dilatation following head injury is important, since in a small carefully selected group of patients a ventricular shunting operation may favourably affect recovery.", "contents": "Ventricular size following head injury: a clinico-radiological study. A retrospective study of the records of 93 patients who had sustained a severe closed head injury was carried out to determine the relationship, if any, between the severity of the injury, the size of the cerebral ventricles and the residual disability. Ventricular size was expressed as a ventricular index. The callosal angle was also measured to see whether this was of value in the recognition of post-traumatic external obstructive hydrocephalus. A normal range of ventricular indices and callosal angles was obtained from a review of the air encephalograms of 56 patients matched for age and sex with the head injury group, and whose air studies were considered to be normal. The findings on the head injury group were subjected to detailed statistical analysis. The study showed that there was no statistically significant relationship between severity of injury, measured by duration of unconsciousness, and ventricular size expressed as a ventricular index. There was a relationship between ventricular size and residual disability as graded in this study. If age was taken into consideration, the relationship between ventricular size and disability was closer. It was not possible to predict residual disability of individual patients from ventricular size alone due to the wide range of values in any disability grade. Failure of air to pass from the basal cisterns over the cerebral convexities is a widely accepted sign of extraventricular obstruction. The shapes of the ventricles, the ventricular index and a callosal angle of 110 degrees or less provided supporting evidence of obstruction in this study. Recognition of an obstructive element in ventricular dilatation following head injury is important, since in a small carefully selected group of patients a ventricular shunting operation may favourably affect recovery."}
{"id": "PMID:1086182", "title": "Coils of the cochlea.", "content": "Abnormalities of the cochlear portion of the bony labyrinth may often be suspected but are inadequately demonstrated by conventional tomography in the antero-posterior position. Further views are necessary. The authors demonstrate this by means of a comparison between tomographic and histological sections of the inner ear of an anencephalic with a unilateral Mundini defect. Submento-vertical tomograms give a good demonstration of the cochlea.", "contents": "Coils of the cochlea. Abnormalities of the cochlear portion of the bony labyrinth may often be suspected but are inadequately demonstrated by conventional tomography in the antero-posterior position. Further views are necessary. The authors demonstrate this by means of a comparison between tomographic and histological sections of the inner ear of an anencephalic with a unilateral Mundini defect. Submento-vertical tomograms give a good demonstration of the cochlea."}
{"id": "PMID:1086190", "title": "Intermediate alpha1-antitrypsin deficiency resulting from a null gene (M-phenotype).", "content": "The presence of a null gene for alpha1-antitrypsin was detected in a family study by the inheritance of intermediate antitrypsin deficiency in association with a normal (PiM) phenotypic pattern. The proband, a 42-year-old man (M-phenotype), was a cigarette smoker and had physiologic evidence of pulmonary emphysema. Three female members of the family were receiving estrogenic medication but had deficient values for serum trypsin inhibitory capacity nevertheless, indicating an unresponsive gene. The mean serum trypsin inhibitory capacity for those with an M-phenotype was significantly lower than that found with an MZ phenotype, presumably due to the total noncontribution to serum antitrypsin activity by the null gene. A quantitative measurement of antitrypsin activity or concentration is necessary in an antitrypsin screening program, since phenotyping procedures alone cannot reveal the null gene.", "contents": "Intermediate alpha1-antitrypsin deficiency resulting from a null gene (M-phenotype). The presence of a null gene for alpha1-antitrypsin was detected in a family study by the inheritance of intermediate antitrypsin deficiency in association with a normal (PiM) phenotypic pattern. The proband, a 42-year-old man (M-phenotype), was a cigarette smoker and had physiologic evidence of pulmonary emphysema. Three female members of the family were receiving estrogenic medication but had deficient values for serum trypsin inhibitory capacity nevertheless, indicating an unresponsive gene. The mean serum trypsin inhibitory capacity for those with an M-phenotype was significantly lower than that found with an MZ phenotype, presumably due to the total noncontribution to serum antitrypsin activity by the null gene. A quantitative measurement of antitrypsin activity or concentration is necessary in an antitrypsin screening program, since phenotyping procedures alone cannot reveal the null gene."}
{"id": "PMID:1086191", "title": "Occlusion of the left main coronary artery secondary to left ventricular angioma.", "content": "Our experience with a patient who had an angioma of the anterior wall of the left ventricle that produced complete occlusion of the left main coronary artery is presented. Diagnosis was made before surgery from findings on cineangiographic studies. Successful surgical treatment consisted of a double aortocoronary bypass to the left anterior descending and obtuse marginal coronary arteries. The angioma was left undisturbed.", "contents": "Occlusion of the left main coronary artery secondary to left ventricular angioma. Our experience with a patient who had an angioma of the anterior wall of the left ventricle that produced complete occlusion of the left main coronary artery is presented. Diagnosis was made before surgery from findings on cineangiographic studies. Successful surgical treatment consisted of a double aortocoronary bypass to the left anterior descending and obtuse marginal coronary arteries. The angioma was left undisturbed."}
{"id": "PMID:1086192", "title": "Right coronary artery dissection. A complication of cardiac catheterization and coronary angiography.", "content": "Four patients are described in whom right coronary artery dissection occurred during retrograde catheterization of the left ventricle or coronary arteries. In two patients, acute myocardial infarction occurred. The possible causes, radiographic features, and clinical implications of this infrequent complication are discussed.", "contents": "Right coronary artery dissection. A complication of cardiac catheterization and coronary angiography. Four patients are described in whom right coronary artery dissection occurred during retrograde catheterization of the left ventricle or coronary arteries. In two patients, acute myocardial infarction occurred. The possible causes, radiographic features, and clinical implications of this infrequent complication are discussed."}
{"id": "PMID:1086196", "title": "Aortocolic fistula, a lethal cause of lower gastrointestinal bleeding: report of a case.", "content": "Aortocolic fistula occurs with spontaneous rupture of aortic and iliac aneurysms into the sigmoid colon, or due to involvement of the aneurysmal wall by acute diverticulitis. In the eight cases reviewed, this complication proved uniformly lethal, although sufficient clinical findings were present for diagnosis, and adequate time was available for a planned therapeutic approach. Lower gastrointestinal bleeding in the patient who has an aortic aneurysm and left-lower-quadrant inflammation suggests the presence of an aortocolic fistual. Angiography should be performed during a bleeding episode to confirm the diagnosis. Surgical correction consists of an axillofemoral by pass graft, excision of the aortic aneurysm, and a Hartmann procedure.", "contents": "Aortocolic fistula, a lethal cause of lower gastrointestinal bleeding: report of a case. Aortocolic fistula occurs with spontaneous rupture of aortic and iliac aneurysms into the sigmoid colon, or due to involvement of the aneurysmal wall by acute diverticulitis. In the eight cases reviewed, this complication proved uniformly lethal, although sufficient clinical findings were present for diagnosis, and adequate time was available for a planned therapeutic approach. Lower gastrointestinal bleeding in the patient who has an aortic aneurysm and left-lower-quadrant inflammation suggests the presence of an aortocolic fistual. Angiography should be performed during a bleeding episode to confirm the diagnosis. Surgical correction consists of an axillofemoral by pass graft, excision of the aortic aneurysm, and a Hartmann procedure."}
{"id": "PMID:1086197", "title": "HL-A antigens in clinical medicine.", "content": "Many autoimmune diseases show a significant association with one or two second segregant series histocompatibility antigens. These associations are of great scientific interest, since they support the concept of HL-A-linked immune-response genes governing specific disease susceptibility. However, with one major exception, the association of HL-A antigens with diseases is not striking enough to provide a worthwhile diagnostic test. The exception is the extraordinarily high incidence of HLA B27 in patients with seronegative spondyloarthropathy best typified by ankylosing spondylitis (AS) and Reiter's disease (RD). In patients with these rheumatic syndromes, the antigen is present in more than 90% of cases compared to an incidence of approximately 6% in normal Caucasians and 4% in black Afro-Americans. The vast majority of rheumatic diseases are readily diagnosable on the basis of a history, physical examination and careful radiographic survey. This applies to most patients with a seronegative spondyloarthropathy, especially when the disease presents as a typical and fully formed clinical syndrome characterized as AS or RD. Sometimes the initial clinical nature may be atypical and only long-term follow-up of the patient will reveal an evolution toward the typical syndrome. In these situations, the correct diagnosis is reinforced by detecting the presence of HLA B27 on the patient's cells. Examination of the patient's family often reveals a high incidence of similar clinical syndromes, nearly always associated with the presence of the antigen. Since tissue typing at the moment is an expensive and relatively unavailable laboratory technic, its widespread and indiscriminatory use as a diagnostic test cannot be encouraged. However, in the clinical settings outlined above, tissue typing provides an invaluable diagnostic test. Presently, the combination of a negative test for rheumatoid factor and a positive test for HLA B27 is one of the strongest diagnostic laboratory profiles available to the physician when faced with a patient with early or atypical rheumatic disease. Aside from the purely clinical setting, the most exciting aspect of the association between these diseases and a specific cell surface antigen lies in the hope that we have a clue to the pathogenesis of a group of common rheumatic disorders. If the cause or causes of spondyloarthropathy can one day be found, the detection of HLA B27 may provide a useful public health measure facilitating preventive medicine. Even now, the detection of susceptible subjects within a family or a population will open the way for early diagnosis and treatment.", "contents": "HL-A antigens in clinical medicine. Many autoimmune diseases show a significant association with one or two second segregant series histocompatibility antigens. These associations are of great scientific interest, since they support the concept of HL-A-linked immune-response genes governing specific disease susceptibility. However, with one major exception, the association of HL-A antigens with diseases is not striking enough to provide a worthwhile diagnostic test. The exception is the extraordinarily high incidence of HLA B27 in patients with seronegative spondyloarthropathy best typified by ankylosing spondylitis (AS) and Reiter's disease (RD). In patients with these rheumatic syndromes, the antigen is present in more than 90% of cases compared to an incidence of approximately 6% in normal Caucasians and 4% in black Afro-Americans. The vast majority of rheumatic diseases are readily diagnosable on the basis of a history, physical examination and careful radiographic survey. This applies to most patients with a seronegative spondyloarthropathy, especially when the disease presents as a typical and fully formed clinical syndrome characterized as AS or RD. Sometimes the initial clinical nature may be atypical and only long-term follow-up of the patient will reveal an evolution toward the typical syndrome. In these situations, the correct diagnosis is reinforced by detecting the presence of HLA B27 on the patient's cells. Examination of the patient's family often reveals a high incidence of similar clinical syndromes, nearly always associated with the presence of the antigen. Since tissue typing at the moment is an expensive and relatively unavailable laboratory technic, its widespread and indiscriminatory use as a diagnostic test cannot be encouraged. However, in the clinical settings outlined above, tissue typing provides an invaluable diagnostic test. Presently, the combination of a negative test for rheumatoid factor and a positive test for HLA B27 is one of the strongest diagnostic laboratory profiles available to the physician when faced with a patient with early or atypical rheumatic disease. Aside from the purely clinical setting, the most exciting aspect of the association between these diseases and a specific cell surface antigen lies in the hope that we have a clue to the pathogenesis of a group of common rheumatic disorders. If the cause or causes of spondyloarthropathy can one day be found, the detection of HLA B27 may provide a useful public health measure facilitating preventive medicine. Even now, the detection of susceptible subjects within a family or a population will open the way for early diagnosis and treatment."}
{"id": "PMID:1086202", "title": "Thyroxine (by competitive protein binding analysis) in human and cow milk and in infant formulas.", "content": "In 45 mothers of full term infants the level of thyroxine (T4) in serum (45 samples) and unskimmed milk (92 samples) was estimated with the aid of competitive protein binding analysis. On the 3rd day after delivery the T4 level in serum was found to be 11.1 +/- 0.4 mug/100 ml, while that in milk was 1.3 +/- 0.3 mug/100 ml. During following days of lactation the T4 concentration in sera decreased. In contrast, however, a gradual increase of the T4 content in milk was observed. The serum and milk levels equalized during the third week post partum (7.3 +/- 1.3 and 7.6 +/- 1.4 mu6/100 ml, respectively). After the 25th day the T4 level in milk was found to be higher than in sera from the same women (paired test: P less than 0.005). Values of 12.9 +/- 1.3 in milk and 8.1 +/- 0.7 mug/100 ml in sera were found in the second month of lactation. A negative correlation between serum and milk T4 level was found during the early lactation (3-20 days), while after 20th day this correlation was positive. Very low and almost undetectable levels of T4 were found in cow's milk and infant formulas derived from it.", "contents": "Thyroxine (by competitive protein binding analysis) in human and cow milk and in infant formulas. In 45 mothers of full term infants the level of thyroxine (T4) in serum (45 samples) and unskimmed milk (92 samples) was estimated with the aid of competitive protein binding analysis. On the 3rd day after delivery the T4 level in serum was found to be 11.1 +/- 0.4 mug/100 ml, while that in milk was 1.3 +/- 0.3 mug/100 ml. During following days of lactation the T4 concentration in sera decreased. In contrast, however, a gradual increase of the T4 content in milk was observed. The serum and milk levels equalized during the third week post partum (7.3 +/- 1.3 and 7.6 +/- 1.4 mu6/100 ml, respectively). After the 25th day the T4 level in milk was found to be higher than in sera from the same women (paired test: P less than 0.005). Values of 12.9 +/- 1.3 in milk and 8.1 +/- 0.7 mug/100 ml in sera were found in the second month of lactation. A negative correlation between serum and milk T4 level was found during the early lactation (3-20 days), while after 20th day this correlation was positive. Very low and almost undetectable levels of T4 were found in cow's milk and infant formulas derived from it."}
{"id": "PMID:1086203", "title": "Specific antidiuretic effect of [Leu4, D-Arg8] vasotocin and [Mpr1, Leu4, D-Arg8] vasotocin. Comment on the idea of lipophilic properties and position 4.", "content": "[Leu4, D-Arg8] vasotocin (I) and [Mpr1, Leu4, D-Arg8] vasotocin (II) possess a considerable and very specific antidiuretic effect. In I, the change of configuration in position 8 caused an increase of the magnitude of the antidiuretic effect. In I, the change of configuration in position 8 caused by two orders and a decrease of the magnitude of the pressor effect by one order. I and II are the first vasotocin analogues reported to have a relatively high and specific antidiuretic effect.", "contents": "Specific antidiuretic effect of [Leu4, D-Arg8] vasotocin and [Mpr1, Leu4, D-Arg8] vasotocin. Comment on the idea of lipophilic properties and position 4. [Leu4, D-Arg8] vasotocin (I) and [Mpr1, Leu4, D-Arg8] vasotocin (II) possess a considerable and very specific antidiuretic effect. In I, the change of configuration in position 8 caused an increase of the magnitude of the antidiuretic effect. In I, the change of configuration in position 8 caused by two orders and a decrease of the magnitude of the pressor effect by one order. I and II are the first vasotocin analogues reported to have a relatively high and specific antidiuretic effect."}
{"id": "PMID:1086204", "title": "Optimization of determination of ovulation after clomiphene therapy.", "content": "A total of 131 cycles of 71 sterility patients, who suffered from anovulatory cycles at least previous 6 months, were monitored daily by a rapid radioimmunoassay for LH and by gynecological examinations before we attempted to induce ovulations with Clomiphene. The width of the cervix, spinnbarkeit, transparency of the cervical mucus and karyopycnotic and acidophilic index of the vagina were recorded daily. 18 subjects exhibited hypoplasia of the uterus and 32 husbands of the 71 patients suffered from severe to medium oligospermia. Homologous inseminations were performed in 15 patients. Different patterns of LH serum levels were observed during and after Clomiphene treatment. The response of LH to Clomiphene and preovulatory LH rise was recorded in 73 cycles. No Clomiphene response with preovulatory LH peak was observed in 31 cycles. 11 patients out of 71 subjects failed to ovulate. 12 patients (30.7%) out of 39 subjects with normospermatic husbands became pregnant. 6 patients (18.7%) of 32 subjects whose husbands suffered from oligospermia became pregnant. A total pregnancy rate of 30% calculated, when the 11 patients with negative responses are excluded. The conclusion is reached that sterility patients benefit most, when both gynecological as well as rapid LH estimations by radioimmunoassay are used to time imminent ovulations in Clomiphene treated subjects.", "contents": "Optimization of determination of ovulation after clomiphene therapy. A total of 131 cycles of 71 sterility patients, who suffered from anovulatory cycles at least previous 6 months, were monitored daily by a rapid radioimmunoassay for LH and by gynecological examinations before we attempted to induce ovulations with Clomiphene. The width of the cervix, spinnbarkeit, transparency of the cervical mucus and karyopycnotic and acidophilic index of the vagina were recorded daily. 18 subjects exhibited hypoplasia of the uterus and 32 husbands of the 71 patients suffered from severe to medium oligospermia. Homologous inseminations were performed in 15 patients. Different patterns of LH serum levels were observed during and after Clomiphene treatment. The response of LH to Clomiphene and preovulatory LH rise was recorded in 73 cycles. No Clomiphene response with preovulatory LH peak was observed in 31 cycles. 11 patients out of 71 subjects failed to ovulate. 12 patients (30.7%) out of 39 subjects with normospermatic husbands became pregnant. 6 patients (18.7%) of 32 subjects whose husbands suffered from oligospermia became pregnant. A total pregnancy rate of 30% calculated, when the 11 patients with negative responses are excluded. The conclusion is reached that sterility patients benefit most, when both gynecological as well as rapid LH estimations by radioimmunoassay are used to time imminent ovulations in Clomiphene treated subjects."}
{"id": "PMID:1086205", "title": "Testosterone in human urine as determined by gas-liquid chromatography.", "content": "A simple and accurate gas chromatographic method for the determination of testosterone in human urine is described. Radioactive testosterone was added to samples to correct methodological loss. A mild acid hydrolysis under protection of benzene layer gave a single estimate of testosterone glucuronate and sulphate. Paper chromatography and rechromatography on chromatoplate served for preliminary qurification. Testosterone was gas chromatographed as acetate and guantitated by the internal standard procedure and flame ionization detector. Results of control experiments, normal values for female and male subjects and estimations in patients with alterations of androgen hormone metabolism are presented.", "contents": "Testosterone in human urine as determined by gas-liquid chromatography. A simple and accurate gas chromatographic method for the determination of testosterone in human urine is described. Radioactive testosterone was added to samples to correct methodological loss. A mild acid hydrolysis under protection of benzene layer gave a single estimate of testosterone glucuronate and sulphate. Paper chromatography and rechromatography on chromatoplate served for preliminary qurification. Testosterone was gas chromatographed as acetate and guantitated by the internal standard procedure and flame ionization detector. Results of control experiments, normal values for female and male subjects and estimations in patients with alterations of androgen hormone metabolism are presented."}
{"id": "PMID:1086206", "title": "Effect of antiandrogen (cyproterone acetate) on the action of testosterone on bone protein.", "content": "The effect of castration, androgen and antiandrogen treatment on bone protein content of femoral diaphyses of male mice was investigated. Castration as well as administration of 2.5 mg of cyproterone acetate for 21 days reduced significantly the protein content of the bone. Testosterone fully compensated the bone protein depletion seen in castrates. Simultaneous administration of both cyproterone acetate and testosterone to castrates blocked the testosterone action. The data show that the mice bone matrix is a highly androgen dependent tissue.", "contents": "Effect of antiandrogen (cyproterone acetate) on the action of testosterone on bone protein. The effect of castration, androgen and antiandrogen treatment on bone protein content of femoral diaphyses of male mice was investigated. Castration as well as administration of 2.5 mg of cyproterone acetate for 21 days reduced significantly the protein content of the bone. Testosterone fully compensated the bone protein depletion seen in castrates. Simultaneous administration of both cyproterone acetate and testosterone to castrates blocked the testosterone action. The data show that the mice bone matrix is a highly androgen dependent tissue."}
{"id": "PMID:1086207", "title": "Distribution of corticotropin releasing factor(s) activity in neural and extraneural tissues of the rat.", "content": "Distribution and dose-response characteristics of corticotropin releasing factor(s) (CRF) activity in the central nervous and extraneural tissues of the rat were examined with a sensitive CRF bioassay using ACTH secretion by cultured rat adenohypophyseal cells. Dose-response curves for hypothalamus were steeper than and not parallel to those for cerebral cortex, liver, serum or human urine. The minimum effective dose was smallest for the posterior pituitary. CRF activity of the basal hypothalamus was considerably higher than that in other parts of the hypothalamus, and was unaltered by 2.5 or 10 min ether stress. Our data indicate that \"specific\" CRF is concentrated primarily in the basal hypothalamus and posterior pituitary and is not identical with widely distributed extrahypothalamic extraneurohypophyseal CRF.", "contents": "Distribution of corticotropin releasing factor(s) activity in neural and extraneural tissues of the rat. Distribution and dose-response characteristics of corticotropin releasing factor(s) (CRF) activity in the central nervous and extraneural tissues of the rat were examined with a sensitive CRF bioassay using ACTH secretion by cultured rat adenohypophyseal cells. Dose-response curves for hypothalamus were steeper than and not parallel to those for cerebral cortex, liver, serum or human urine. The minimum effective dose was smallest for the posterior pituitary. CRF activity of the basal hypothalamus was considerably higher than that in other parts of the hypothalamus, and was unaltered by 2.5 or 10 min ether stress. Our data indicate that \"specific\" CRF is concentrated primarily in the basal hypothalamus and posterior pituitary and is not identical with widely distributed extrahypothalamic extraneurohypophyseal CRF."}
{"id": "PMID:1086208", "title": "Studies on factor VIII activation potential in hemophilia A-plasma and its significance for the comprehension of hemophilia.", "content": "In a factor VIII exchange test experiment higher factor VIII activities are measurable than would be expected from the single activities of the used plasmas. The same goes for the use of plasmas from carriers of hemophilia A, but not for plasmas from patients with v.W.J.S. (von-Willebrand J\u00fcrgens syndrome). In plasmas from hemophilic adults activation obviously proceeds at a slower rate than in plasmas from hemophilic children. The experimental results lead to the hypothesis that factor VIII for its activation needs a specific activator or activator system. This process can be stopped by natural inhibitors. Contrary to existing opinion, the coagulation defect of hemophilia A is not to be sought in an inability to be activated or even in absence of factor VIII, but in a previous step, in its activator. This activator is absent or defective. In patients with v.W.J.S. disease the activator or activator system is intact, but factor VIII is missing.", "contents": "Studies on factor VIII activation potential in hemophilia A-plasma and its significance for the comprehension of hemophilia. In a factor VIII exchange test experiment higher factor VIII activities are measurable than would be expected from the single activities of the used plasmas. The same goes for the use of plasmas from carriers of hemophilia A, but not for plasmas from patients with v.W.J.S. (von-Willebrand J\u00fcrgens syndrome). In plasmas from hemophilic adults activation obviously proceeds at a slower rate than in plasmas from hemophilic children. The experimental results lead to the hypothesis that factor VIII for its activation needs a specific activator or activator system. This process can be stopped by natural inhibitors. Contrary to existing opinion, the coagulation defect of hemophilia A is not to be sought in an inability to be activated or even in absence of factor VIII, but in a previous step, in its activator. This activator is absent or defective. In patients with v.W.J.S. disease the activator or activator system is intact, but factor VIII is missing."}
{"id": "PMID:1086211", "title": "Studies on the mitogenic activity of trypsin, pronase and neuraminidase on human peripheral blood lymphocytes.", "content": "Human peripheral blood lymphocytes were cultured in the presence of protease (trypsin and pronase) and Vibrio choleraneuraminidase. T and B lymphocyte populations were separated and the effect of these enzymes plus phytohaemagglutin or Tuberculin was studied. The results of these experiments show that proteases moderately stimulate spontaneous deoxynucleic acid synthesis of control cells and potentiate the effect of tuberculin on sensitized cells. These enzymes act specifically on B lymphocytes. Neuraminidase also increases spontaneous deoxyribonucleic acid synthesis of control cells and augments significantly the in vitro response to PPD. There is no additive effect of neuraminidase on phytohaemagglutin stimulated cells. Neuraminidase seems to stimulate specifically T lymphocytes. Some possible mechanisms of action of these enzymes are proposed and discussed.", "contents": "Studies on the mitogenic activity of trypsin, pronase and neuraminidase on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were cultured in the presence of protease (trypsin and pronase) and Vibrio choleraneuraminidase. T and B lymphocyte populations were separated and the effect of these enzymes plus phytohaemagglutin or Tuberculin was studied. The results of these experiments show that proteases moderately stimulate spontaneous deoxynucleic acid synthesis of control cells and potentiate the effect of tuberculin on sensitized cells. These enzymes act specifically on B lymphocytes. Neuraminidase also increases spontaneous deoxyribonucleic acid synthesis of control cells and augments significantly the in vitro response to PPD. There is no additive effect of neuraminidase on phytohaemagglutin stimulated cells. Neuraminidase seems to stimulate specifically T lymphocytes. Some possible mechanisms of action of these enzymes are proposed and discussed."}
{"id": "PMID:1086212", "title": "Generation of antibody diversity. IV. Variation within single clones of antibody-forming cells developing in vivo.", "content": "Previous experimental work demonstrated that clonal variation occurs in vitro. The present experiments were designed to test for clonal variation in vivo. B cells were transferred at limiting dilution, with antigen, into irradiated recipients. Seven days later spleens were assayed for plaque-forming cell (PFC) colonies. Control experiments showed that these PFC colonies were clones, that is, they were derived from a single B cell precursor. When the clones were analyzed for heterogeneity of the PFC population, using cross-reactivity on various mixtures of red blood cells as a method of detecting differences in antibody specificity, from 23-83% of the clones contained variants. By adjusting the amounts of helper activity and antigen available to a developing clone, we have been able to influence this variation; high levels of help and/or antigen favor pure clones, while low levels of either produce mainly mixed clones.", "contents": "Generation of antibody diversity. IV. Variation within single clones of antibody-forming cells developing in vivo. Previous experimental work demonstrated that clonal variation occurs in vitro. The present experiments were designed to test for clonal variation in vivo. B cells were transferred at limiting dilution, with antigen, into irradiated recipients. Seven days later spleens were assayed for plaque-forming cell (PFC) colonies. Control experiments showed that these PFC colonies were clones, that is, they were derived from a single B cell precursor. When the clones were analyzed for heterogeneity of the PFC population, using cross-reactivity on various mixtures of red blood cells as a method of detecting differences in antibody specificity, from 23-83% of the clones contained variants. By adjusting the amounts of helper activity and antigen available to a developing clone, we have been able to influence this variation; high levels of help and/or antigen favor pure clones, while low levels of either produce mainly mixed clones."}
{"id": "PMID:1086213", "title": "The role of thymus-derived lymphocytes in an antibody-mediated hapten-specific helper effect.", "content": "The cellular basis of the antibody-mediated, hapten-specific anti-idiotype antibody response of CBA/J mice to a hapten conjugate of the BALB/c myeloma protein LPC-1 was examined. Previous studies have shown that anti-idiotype antibody is produced if the mice are given anti-2,4-dinitrophenyl (DNP) antibody and then immunized with DNP-LPC-1; immunization with DNP-LPC-1 or LPC-1 alone does not lead to the production of this antibody. In the present experiments, mice depleted of thymus-derived lymphocytes by two different techniques failed to make anti-idiotype antibody after transfer of anti-DNP antibody and immunization with DNP-LPC-1. However, thymus-deprived mice given anti-DNP antibody and reconstituted with thymus-derived spleen cells did make anti-idiotype antibody in response to DNP-LPC-1. Thymocytes could not reconstitute such mice for this response. The possible mechanisms for this hapten-specific, antibody-mediated, T cell-dependent helper effect are discussed. It is felt that such helper effects represent an alternate pathway to conventional specific T cell-mediated cooperation. Further examples of antibody-mediated helper effects which support this hypothesis are also presented.", "contents": "The role of thymus-derived lymphocytes in an antibody-mediated hapten-specific helper effect. The cellular basis of the antibody-mediated, hapten-specific anti-idiotype antibody response of CBA/J mice to a hapten conjugate of the BALB/c myeloma protein LPC-1 was examined. Previous studies have shown that anti-idiotype antibody is produced if the mice are given anti-2,4-dinitrophenyl (DNP) antibody and then immunized with DNP-LPC-1; immunization with DNP-LPC-1 or LPC-1 alone does not lead to the production of this antibody. In the present experiments, mice depleted of thymus-derived lymphocytes by two different techniques failed to make anti-idiotype antibody after transfer of anti-DNP antibody and immunization with DNP-LPC-1. However, thymus-deprived mice given anti-DNP antibody and reconstituted with thymus-derived spleen cells did make anti-idiotype antibody in response to DNP-LPC-1. Thymocytes could not reconstitute such mice for this response. The possible mechanisms for this hapten-specific, antibody-mediated, T cell-dependent helper effect are discussed. It is felt that such helper effects represent an alternate pathway to conventional specific T cell-mediated cooperation. Further examples of antibody-mediated helper effects which support this hypothesis are also presented."}
{"id": "PMID:1086214", "title": "Spontaneous release of T cell receptors for alloantigens. II. Induction of antibodies to T cell receptors.", "content": "Spleen cells from normal mice, when cultivated in vitro, released receptors or recognition structures (RS) for alloantigens into the surrounding medium. The spontaneous shedding of receptors was revealed by their ability to induce the formation of anti-recognition structure(anti-RS) antibodies upon injection into appropriate F1 hybrid recipients. Cell suspensions containing T and B lymphocytes and those containing T lymphocytes were capable of inducing anti-RS antibody formation, whereas suspensions devoid of T cells were incapable of doing so. Receptors shed from such cell suspensions during a 24-hour cultivation period gave exactly the same results. Cell-free culture supernatants, however, incited higher anti-RS antibody titers, presumably because of an accumulation of RS. The capacity of released T cell receptors to recognize alloantigens as determined in the PAR assay and their ability to induce anti-RS antibodies went roughly parallel.", "contents": "Spontaneous release of T cell receptors for alloantigens. II. Induction of antibodies to T cell receptors. Spleen cells from normal mice, when cultivated in vitro, released receptors or recognition structures (RS) for alloantigens into the surrounding medium. The spontaneous shedding of receptors was revealed by their ability to induce the formation of anti-recognition structure(anti-RS) antibodies upon injection into appropriate F1 hybrid recipients. Cell suspensions containing T and B lymphocytes and those containing T lymphocytes were capable of inducing anti-RS antibody formation, whereas suspensions devoid of T cells were incapable of doing so. Receptors shed from such cell suspensions during a 24-hour cultivation period gave exactly the same results. Cell-free culture supernatants, however, incited higher anti-RS antibody titers, presumably because of an accumulation of RS. The capacity of released T cell receptors to recognize alloantigens as determined in the PAR assay and their ability to induce anti-RS antibodies went roughly parallel."}
{"id": "PMID:1086215", "title": "Surface morphology of murine B and T lymphocytes: A comparative study by scanning electron microscopy.", "content": "A variety of murine lymphocytes of known B or T derivation obtained from different lymphoid organs were prepared for scanning electron microscopy (SEM) by the critical point drying method after collecting the cells by aspiration onto silver membranes. Comparison of SEM appearances of cells prepared by this technique and serological classification according to surface antigens showed that most T cells had smooth surfaces with few microvilli, while many B lymphocytes were moderately to markedly villous. Further evidence for the above correlation was obtained by examining thymic cells and enriched B or T cell populations. Thymic cell suspensions containing less than 5% B cells showed over 80% generally smooth cells by SEM. Enriched T cell populations, obtained by mass cytolysis of lymph node preparations with anti-Ia or anti-Ig sera or by purification through nylon fiber columns, contained over 85% T cells, and more than 75% of them were of the smooth cell type. A similar correlation was noted for enriched B cell populations obtained by cytolysis of lymph node cells with anti-Thy-1 serum, and by lysis of EAC-rosettes. Over 90% of these cells were identified as B cells by immunologic methods and approximately 75% had moderate to markedly villous surfaces. The 15% difference can be accounted for by the existence of a subpopulation of smooth B cells. Direct observation of EAC-rosettes confirmed that most B cells had moderate to large numbers of surface microvilli and that less than 10% were smooth. It is possible that some of the smooth cells seen in enriched B cell populations may represent precursors or B lymphocytes at different stages of differentiation. These results indicate that murine T and B lymphocytes, like their human counterparts, can be recognized in many cases under the SEM on the basis of their surface morphology. Smoother B and more villous T cells are difficult to classify by SEM without parallel immunologic identification.", "contents": "Surface morphology of murine B and T lymphocytes: A comparative study by scanning electron microscopy. A variety of murine lymphocytes of known B or T derivation obtained from different lymphoid organs were prepared for scanning electron microscopy (SEM) by the critical point drying method after collecting the cells by aspiration onto silver membranes. Comparison of SEM appearances of cells prepared by this technique and serological classification according to surface antigens showed that most T cells had smooth surfaces with few microvilli, while many B lymphocytes were moderately to markedly villous. Further evidence for the above correlation was obtained by examining thymic cells and enriched B or T cell populations. Thymic cell suspensions containing less than 5% B cells showed over 80% generally smooth cells by SEM. Enriched T cell populations, obtained by mass cytolysis of lymph node preparations with anti-Ia or anti-Ig sera or by purification through nylon fiber columns, contained over 85% T cells, and more than 75% of them were of the smooth cell type. A similar correlation was noted for enriched B cell populations obtained by cytolysis of lymph node cells with anti-Thy-1 serum, and by lysis of EAC-rosettes. Over 90% of these cells were identified as B cells by immunologic methods and approximately 75% had moderate to markedly villous surfaces. The 15% difference can be accounted for by the existence of a subpopulation of smooth B cells. Direct observation of EAC-rosettes confirmed that most B cells had moderate to large numbers of surface microvilli and that less than 10% were smooth. It is possible that some of the smooth cells seen in enriched B cell populations may represent precursors or B lymphocytes at different stages of differentiation. These results indicate that murine T and B lymphocytes, like their human counterparts, can be recognized in many cases under the SEM on the basis of their surface morphology. Smoother B and more villous T cells are difficult to classify by SEM without parallel immunologic identification."}
{"id": "PMID:1086216", "title": "The effect of specific cell inactivation on the cellular immune response to ferredoxin peptides.", "content": "Previous studies using synthetic immunogenic molecules containing two haptenic peptides (ther NH2-terminal heptapeptide and the COOH-terminal pentapeptide of oxidized ferredoxin (O-Fd) from C. pasteurianum) have shown that both peptides individually are capable of initiating lymphocyte transformation and inhibiting migration in populations of lymphocytes from O-Fd-sensitized guinea pigs. While migration inhibition could readily be stimulated by single haptenic peptides, lymphocyte treasformation appeared to be more easily induced by molecules containing two or more haptenic peptides (these could be identical or different) (Kelly, B., Levy, J.G. and Hull, D., Eur. J. Immunol., 1973. 3:574). If lymphocyte transformation is a T cell-mediated phenomenon, these observations indicate the possibility of T cell-T cell interaction. The two haptenic peptides (designated \"N\" and \"C\") were synthesized and conjugated to succinylated bovine serum albumin (S-BSA), forming the conjugates N-S-BSA and C-S-BSA, respectively. These conjugates were labeled to high specific activity with 125iodine and were used in an \"antigen suicide\" procedure to treat guinea pig lymph node cell preparations previously sensitized to O-Fd and keyhole limpet hemocyanin (KLH). Cell populations exposed to either 125I-labeled C-S-BCA demonstrated decreased lymphocyte transformation in the presence of O-Fd but not in the presence of KLH. These results indicate specific cell inactivation by the radioactive peptide conjugates of those cells responsible for initiating cell transformation. Experiments performed by mixing 125I-labeled N-S-BSA-treated cells with 125I-labeled C-S-BSA-treated cells were successful in partially restoring the response to O-Fd and suggest possible synergy between N and C determinant binding cells in the cellular immune response to O-Fd. Evidence from B cell depletion studies suggests that this is a T cell-T cell interaction.", "contents": "The effect of specific cell inactivation on the cellular immune response to ferredoxin peptides. Previous studies using synthetic immunogenic molecules containing two haptenic peptides (ther NH2-terminal heptapeptide and the COOH-terminal pentapeptide of oxidized ferredoxin (O-Fd) from C. pasteurianum) have shown that both peptides individually are capable of initiating lymphocyte transformation and inhibiting migration in populations of lymphocytes from O-Fd-sensitized guinea pigs. While migration inhibition could readily be stimulated by single haptenic peptides, lymphocyte treasformation appeared to be more easily induced by molecules containing two or more haptenic peptides (these could be identical or different) (Kelly, B., Levy, J.G. and Hull, D., Eur. J. Immunol., 1973. 3:574). If lymphocyte transformation is a T cell-mediated phenomenon, these observations indicate the possibility of T cell-T cell interaction. The two haptenic peptides (designated \"N\" and \"C\") were synthesized and conjugated to succinylated bovine serum albumin (S-BSA), forming the conjugates N-S-BSA and C-S-BSA, respectively. These conjugates were labeled to high specific activity with 125iodine and were used in an \"antigen suicide\" procedure to treat guinea pig lymph node cell preparations previously sensitized to O-Fd and keyhole limpet hemocyanin (KLH). Cell populations exposed to either 125I-labeled C-S-BCA demonstrated decreased lymphocyte transformation in the presence of O-Fd but not in the presence of KLH. These results indicate specific cell inactivation by the radioactive peptide conjugates of those cells responsible for initiating cell transformation. Experiments performed by mixing 125I-labeled N-S-BSA-treated cells with 125I-labeled C-S-BSA-treated cells were successful in partially restoring the response to O-Fd and suggest possible synergy between N and C determinant binding cells in the cellular immune response to O-Fd. Evidence from B cell depletion studies suggests that this is a T cell-T cell interaction."}
{"id": "PMID:1086217", "title": "Receptors for IgG molecules on human lymphocytes forming spontaneous rosettes with sheep red cells.", "content": "A mixed rosette technique with sheep red blood cells (SRBC) and ox erythrocytes heavily coated with rabbit antibody (EA) was employed to simultaneously identify human peripheral blood T lymphocytes and IgG receptor-bearing cells. The findings of a noticeable proportion of \"mixed rosettes\" in peripheral blood lymphocytes freshly drawn from normal individuals and of an even higher number of these mixed rosettes in cell suspension kept in culture media supplemented with fetal calf serum provide evidence for the capacity of human T cells to express membrane receptors for antigen-antibody complexes.", "contents": "Receptors for IgG molecules on human lymphocytes forming spontaneous rosettes with sheep red cells. A mixed rosette technique with sheep red blood cells (SRBC) and ox erythrocytes heavily coated with rabbit antibody (EA) was employed to simultaneously identify human peripheral blood T lymphocytes and IgG receptor-bearing cells. The findings of a noticeable proportion of \"mixed rosettes\" in peripheral blood lymphocytes freshly drawn from normal individuals and of an even higher number of these mixed rosettes in cell suspension kept in culture media supplemented with fetal calf serum provide evidence for the capacity of human T cells to express membrane receptors for antigen-antibody complexes."}
{"id": "PMID:1086218", "title": "\"Natural\" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell.", "content": "Normal mice contain cytolytic cells with specificity for in vitro grown mouse Moloney leukemia cells. Such killer cells are most frequent in the spleens; lymph node and bone marrow contain less and thymus virtually no killer activity. Peak activity is found around one to three months of age. Spleen cells from genetically athymic mice are as active killer cells as those from normal mice of the same strain. Treatment with anti-theta serum plus complement followed by removal of adherent and surface Ig positive cells by filtration through anti-Ig columns will leave between 1-5% of the original spleen cell population from a normal mouse. These cells have the morphology of small lymphocytes and perhaps contain all of the total original killer activity of the spleen against the Moloney leukemia cells. Such killer enriched cells are devoid of T and B lymphocytes and largely fail to function in antibody induced, cell-mediated lysis against antibody-coated chicken erythrocytes. It is concluded that the spontaneous selective cytotoxic activity of normal mouse spleen cells against Moloney leukemia cells is exerted by small lymphocytes of yet undefined nature.", "contents": "\"Natural\" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Normal mice contain cytolytic cells with specificity for in vitro grown mouse Moloney leukemia cells. Such killer cells are most frequent in the spleens; lymph node and bone marrow contain less and thymus virtually no killer activity. Peak activity is found around one to three months of age. Spleen cells from genetically athymic mice are as active killer cells as those from normal mice of the same strain. Treatment with anti-theta serum plus complement followed by removal of adherent and surface Ig positive cells by filtration through anti-Ig columns will leave between 1-5% of the original spleen cell population from a normal mouse. These cells have the morphology of small lymphocytes and perhaps contain all of the total original killer activity of the spleen against the Moloney leukemia cells. Such killer enriched cells are devoid of T and B lymphocytes and largely fail to function in antibody induced, cell-mediated lysis against antibody-coated chicken erythrocytes. It is concluded that the spontaneous selective cytotoxic activity of normal mouse spleen cells against Moloney leukemia cells is exerted by small lymphocytes of yet undefined nature."}
{"id": "PMID:1086219", "title": "Cell-medicated cytotoxicity against ectromelia virus-infected target cells. III. Role of the H-2 gene complex.", "content": "The role of the H-2 gene complex in expression of cytotoxicity exerted by specific ectromelia-immune thymus-derived (T) cells against ectromelia-infected target cells was examined. A repertoire of inbred mouse strains (some congenic) including the H-2 haplotypes k, d, b, s, q, the recombinant H-2a(k/d) and F1 hybrids (k/b and d/b) were immunized with virus and their spleen cells tested 6 days later, at the peak of the primary response, against H-2k,H-2d and H-2b target cells. Significant specific cytotoxicity occurred only when the immune cell donors and the target cells shared all or part of the same H-2 gene complex. For example, H-2a (k/d) immune cells killed both H-2k and H-2d target cells. There was no detectable effect of the non-H-2 genetic background, H-2 public specificities, or the M-locus. Target cells infected with ectromelia virus exhibited quantitative or qualitative changes (or both) in expression of normal H-2 antigens as indicated by reduced susceptibility to killing by T cells activated against H-2 antigens in mixed lymphocyte culture. These data are consistent with the hypothesis that T cells in this system are responding to virus-induced, specific changes in antigens on infected cells which are controlled by genes in the H-2 complex; these genes seem likely to be those coding for H-2 private specificities, or genes closely linked to them.", "contents": "Cell-medicated cytotoxicity against ectromelia virus-infected target cells. III. Role of the H-2 gene complex. The role of the H-2 gene complex in expression of cytotoxicity exerted by specific ectromelia-immune thymus-derived (T) cells against ectromelia-infected target cells was examined. A repertoire of inbred mouse strains (some congenic) including the H-2 haplotypes k, d, b, s, q, the recombinant H-2a(k/d) and F1 hybrids (k/b and d/b) were immunized with virus and their spleen cells tested 6 days later, at the peak of the primary response, against H-2k,H-2d and H-2b target cells. Significant specific cytotoxicity occurred only when the immune cell donors and the target cells shared all or part of the same H-2 gene complex. For example, H-2a (k/d) immune cells killed both H-2k and H-2d target cells. There was no detectable effect of the non-H-2 genetic background, H-2 public specificities, or the M-locus. Target cells infected with ectromelia virus exhibited quantitative or qualitative changes (or both) in expression of normal H-2 antigens as indicated by reduced susceptibility to killing by T cells activated against H-2 antigens in mixed lymphocyte culture. These data are consistent with the hypothesis that T cells in this system are responding to virus-induced, specific changes in antigens on infected cells which are controlled by genes in the H-2 complex; these genes seem likely to be those coding for H-2 private specificities, or genes closely linked to them."}
{"id": "PMID:1086220", "title": "Five types of lymphocytes (Ig-theta-, Ig-theta+weak, Ig-theta+strong, Ig+theta- and Ig+theta+) characterized by double immunofluorescence and electrophoretic mobility. Organ distribution in normal and nude mice.", "content": "The simultaneous detection of Ig and theta on the surface of mouse lymphocytes permits the detection of four cell types (Ig-theta-, Ig-theta+, Ig+theta-, and Ig+theta+) which also have distinct electrophoretic profiles. All types are present in variable proportions in all lymphoid organs studied. Results obtained in congenitally athymic nude mice and in T-deprived mice define a new type of lymphocyte, the Ig-theta+weak. This cell is non-recirculating, Ig-, with a low density of theta and a slow electrophoretic mobility. It is a candidate for a T-committed prethymic cell.", "contents": "Five types of lymphocytes (Ig-theta-, Ig-theta+weak, Ig-theta+strong, Ig+theta- and Ig+theta+) characterized by double immunofluorescence and electrophoretic mobility. Organ distribution in normal and nude mice. The simultaneous detection of Ig and theta on the surface of mouse lymphocytes permits the detection of four cell types (Ig-theta-, Ig-theta+, Ig+theta-, and Ig+theta+) which also have distinct electrophoretic profiles. All types are present in variable proportions in all lymphoid organs studied. Results obtained in congenitally athymic nude mice and in T-deprived mice define a new type of lymphocyte, the Ig-theta+weak. This cell is non-recirculating, Ig-, with a low density of theta and a slow electrophoretic mobility. It is a candidate for a T-committed prethymic cell."}
{"id": "PMID:1086221", "title": "Cell-mediated immunity to Moloney sarcoma virus in mice. II. Analysis of antigenic specificities involved in T lymphocyte-mediated in vivo rejection of murine sarcoma virus-induced tumors.", "content": "Sublethally irradiated BALB/c mice innoculated with Moloney sarcoma virus (MSVm) develop progressively growing tumors and die within 30 days of virus innoculation. These animals can be protected from tumor progression (and death) by innoculation of small numbers of MSV-immune T lymphocytes from MSV-M innoculated (but unirradiated) animals. T lymphocytes in these donor animals have been shown to express immunity to a variety of viral and virally-induced antigens. We have investigated whether immunity to any one of these antigens was critically important in leading to protection of the irradiated animals by sensitizing normal T lymphocytes in vitro to different viral antigens and examining the ability of these sensitized cells to protect the irradiated recipients. Data is presented to show that cells sensitized in vitro to MSV-transformed fibroblasts, and to purified antigens with group specificity, but not to viral envelope antigens, or whole virus, are capable of protecting the irradiated MS innoculated animals.", "contents": "Cell-mediated immunity to Moloney sarcoma virus in mice. II. Analysis of antigenic specificities involved in T lymphocyte-mediated in vivo rejection of murine sarcoma virus-induced tumors. Sublethally irradiated BALB/c mice innoculated with Moloney sarcoma virus (MSVm) develop progressively growing tumors and die within 30 days of virus innoculation. These animals can be protected from tumor progression (and death) by innoculation of small numbers of MSV-immune T lymphocytes from MSV-M innoculated (but unirradiated) animals. T lymphocytes in these donor animals have been shown to express immunity to a variety of viral and virally-induced antigens. We have investigated whether immunity to any one of these antigens was critically important in leading to protection of the irradiated animals by sensitizing normal T lymphocytes in vitro to different viral antigens and examining the ability of these sensitized cells to protect the irradiated recipients. Data is presented to show that cells sensitized in vitro to MSV-transformed fibroblasts, and to purified antigens with group specificity, but not to viral envelope antigens, or whole virus, are capable of protecting the irradiated MS innoculated animals."}
{"id": "PMID:1086222", "title": "The failure to show a necessary role for C3 in the in vitro antibody response.", "content": "The in vitro antibody response of mouse spleen cells to TNP coupled to both T-dependent and T-independent carriers as well as to sheep erythrocytes has been studied to investigate the possible role of complement activation in the induction of antibody formation. The following has been found. (1) In vitro responses of both IgM and IgG can be obtained to both T-dependent and T-independent antigens in serum-free media, although they are smaller than those found in serum-containing media. This shows that no exogenous source of complement is necessary for in vitro antibody formation by spleen cells. (2) Similarly, normal antibody responses are obtained if the cultures are grown in human serum depleted of C3b-inactivator, which contains high concentrations of C3b. (3) In the presence of antibody to mouse C3 the response to the T-independent antigen is reduced, the IgM responses being more affected than the IgG. However, purified F(ab')2 anti-C3 has no inhibitory effect and it therefore seems likely that it is the formation of intact immune complexes containing Fc rather than the interference with C3 function that is responsible for the inhibition seen. (4) The conventionally purified anti-complementary factor from cobra venom has no effect on the antibody response in serum-free culture or when human or fetal calf sera are used. In no experiment was any potentiation of T-dependent responses observed. However, the presence of quite small concentrations (2%) of cobra veno", "contents": "The failure to show a necessary role for C3 in the in vitro antibody response. The in vitro antibody response of mouse spleen cells to TNP coupled to both T-dependent and T-independent carriers as well as to sheep erythrocytes has been studied to investigate the possible role of complement activation in the induction of antibody formation. The following has been found. (1) In vitro responses of both IgM and IgG can be obtained to both T-dependent and T-independent antigens in serum-free media, although they are smaller than those found in serum-containing media. This shows that no exogenous source of complement is necessary for in vitro antibody formation by spleen cells. (2) Similarly, normal antibody responses are obtained if the cultures are grown in human serum depleted of C3b-inactivator, which contains high concentrations of C3b. (3) In the presence of antibody to mouse C3 the response to the T-independent antigen is reduced, the IgM responses being more affected than the IgG. However, purified F(ab')2 anti-C3 has no inhibitory effect and it therefore seems likely that it is the formation of intact immune complexes containing Fc rather than the interference with C3 function that is responsible for the inhibition seen. (4) The conventionally purified anti-complementary factor from cobra venom has no effect on the antibody response in serum-free culture or when human or fetal calf sera are used. In no experiment was any potentiation of T-dependent responses observed. However, the presence of quite small concentrations (2%) of cobra veno"}
{"id": "PMID:1086223", "title": "Differing mechanisms of tolerance and desensitization to dinitrochlorobenzene in guinea pigs.", "content": "In the present paper the mechanisms of tolerance and desensitization to dinitrochlorobenzene (DNCB) contact sensitivity in guinea pigs were investigated using the methods of adoptive sensitization of tolerant and normal syngeneic recipients and cyclophosphamide-treatment of tolerant animals known to selectively inactivate suppressor lymphocytes. It was shown that desensitization of presensitized animals is caused by the direct effect of the intravenously injected hapten on the effector cells in the peripheral compartment. The immediate onset of unresponsiveness and its very short duration almost exclude the possible involvement of enhancing antibodies or suppressor cells. In the case of tolerance induced by pretreatment with dinitrobenzenesulfonic acid, suppressor cell activity is enhanced, preventing normal specific immunocompetent cells from recognizing the antigen and/or proliferating in the draining lymph nodes. Whether suppressor lymphocytes are of the B or T type is not yet known.", "contents": "Differing mechanisms of tolerance and desensitization to dinitrochlorobenzene in guinea pigs. In the present paper the mechanisms of tolerance and desensitization to dinitrochlorobenzene (DNCB) contact sensitivity in guinea pigs were investigated using the methods of adoptive sensitization of tolerant and normal syngeneic recipients and cyclophosphamide-treatment of tolerant animals known to selectively inactivate suppressor lymphocytes. It was shown that desensitization of presensitized animals is caused by the direct effect of the intravenously injected hapten on the effector cells in the peripheral compartment. The immediate onset of unresponsiveness and its very short duration almost exclude the possible involvement of enhancing antibodies or suppressor cells. In the case of tolerance induced by pretreatment with dinitrobenzenesulfonic acid, suppressor cell activity is enhanced, preventing normal specific immunocompetent cells from recognizing the antigen and/or proliferating in the draining lymph nodes. Whether suppressor lymphocytes are of the B or T type is not yet known."}
{"id": "PMID:1086224", "title": "The failure to show a necessary role for C3 in the in vitro antibody response.", "content": "The in vitro antibody response of mouse spleen cells to TNP coupled to both T-dependent and T-independent carriers as well as to sheep erythrocytes has been studied to investigate the possible role of complement activation in the induction of antibody formation. The following has been found. (1) In vitro responses of both IgM and IgG can be obtained to both T-dependent and T-independent antigens in serum-free media, although they are smaller than those found in serum-containing media. This shows that no exogenous source of complement is necessary for in vitro antibody formation by spleen cells. (2) Similarly, normal antibody responses are obtained if the cultures are grown in human serum depleted of C3b-inactivator, which contains high concentrations of C3b. (3) In the presence of antibody to mouse C3 the response to the T-independent antigen is reduced, the IgM responses being more affected than the IgG. However, purified F(ab')2 anti-C3 has no inhibitory effect and it therefore seems likely that it is the formation of intact immune complexes containing Fc rather than the interference with C3 function that is responsible for the inhibition seen. (4) The conventionally purified anti-complementary factor from cobra venom has no effect on the antibody response in serum-free culture or when human or fetal calf sera are used. In no experiment was any potentiation of T-dependent responses observed. However, the presence of quite small concentrations (2%) of cobra venom factor (CVF)-treated normal mouse serum produced a profound inhibition of the antibody responses, affecting IgG responses more than IgM, and being T-dependent more than T-independent, but no part of the response being unaffected. However, CVF heated to 70 degrees C which destroys the anticomplementary activity but not the contaminating phospholipase A was equally effective. More highly purified CVF, on the other hand, was not. These findings strongly suggest that the inhibition observed is due to residual phospholipase A reacting with lecithin in the mouse serum rather than to any anticomplementary effect. (5) Spleen cells taken from mice-treated with CVF in vivo were also unable to give a full IgG response to the T-dependent antigens if challenged 24 h after CVF treatment. However, after 48 h no inhibition was observed. It therefore seems to us that it is unlikely that C3 plays any real part in the in vitro antibody response. Our data--like the occurrence of genetically C3-deficient humans with apparently normal antibody responses--sould seem to preclude any necessary role for C3 in antibody production.", "contents": "The failure to show a necessary role for C3 in the in vitro antibody response. The in vitro antibody response of mouse spleen cells to TNP coupled to both T-dependent and T-independent carriers as well as to sheep erythrocytes has been studied to investigate the possible role of complement activation in the induction of antibody formation. The following has been found. (1) In vitro responses of both IgM and IgG can be obtained to both T-dependent and T-independent antigens in serum-free media, although they are smaller than those found in serum-containing media. This shows that no exogenous source of complement is necessary for in vitro antibody formation by spleen cells. (2) Similarly, normal antibody responses are obtained if the cultures are grown in human serum depleted of C3b-inactivator, which contains high concentrations of C3b. (3) In the presence of antibody to mouse C3 the response to the T-independent antigen is reduced, the IgM responses being more affected than the IgG. However, purified F(ab')2 anti-C3 has no inhibitory effect and it therefore seems likely that it is the formation of intact immune complexes containing Fc rather than the interference with C3 function that is responsible for the inhibition seen. (4) The conventionally purified anti-complementary factor from cobra venom has no effect on the antibody response in serum-free culture or when human or fetal calf sera are used. In no experiment was any potentiation of T-dependent responses observed. However, the presence of quite small concentrations (2%) of cobra venom factor (CVF)-treated normal mouse serum produced a profound inhibition of the antibody responses, affecting IgG responses more than IgM, and being T-dependent more than T-independent, but no part of the response being unaffected. However, CVF heated to 70 degrees C which destroys the anticomplementary activity but not the contaminating phospholipase A was equally effective. More highly purified CVF, on the other hand, was not. These findings strongly suggest that the inhibition observed is due to residual phospholipase A reacting with lecithin in the mouse serum rather than to any anticomplementary effect. (5) Spleen cells taken from mice-treated with CVF in vivo were also unable to give a full IgG response to the T-dependent antigens if challenged 24 h after CVF treatment. However, after 48 h no inhibition was observed. It therefore seems to us that it is unlikely that C3 plays any real part in the in vitro antibody response. Our data--like the occurrence of genetically C3-deficient humans with apparently normal antibody responses--sould seem to preclude any necessary role for C3 in antibody production."}
{"id": "PMID:1086225", "title": "Immunological memory function of the T and B cell types: distribution over mouse spleen and lymph nodes.", "content": "Spleens from LAF1 mice injected intravenously with sheep erythrocytes (SE) are relatively rich in memory T cells early in the immune response (1 to 3 days) and rich in memory B cells as the response progresses (2 weeks or more). Marked cooperation for the secondary immune response in vitro was obtained by combining 10(6) spleen cells from LAF1 mice, taken 2 days after intravenous priming with SE, with 10(7) spleen cells from day 14 primed mice. The results indicate relative deficiencies in the spleen for B memory cells on days 1 to 2 and for T memory cells on day 14 after priming. Day -14, but not day -2, immune lymph node (LN) cells could replace the day -2 spleen cells (anti-Thy 1.2 sensitive) in the in vitro cooperation with day -14 immune spleen cells. Immune spleen cells taken 4 to 7 days after priming contain more equivalent numbers of B and T memory cells, but 10 to 7 days after transfer of such immune spleen cells without SE into irradiated recipients the T memory cells were again more prominent in lymph node and the B memory cells in spleen as shown by in vitro cooperation studies. These results suggest that during the second week after intravenous injection of SE relatively more T than B memory cells migrate from spleen to lymph node, resulting in an imbalance in the splenic memory cell population favoring B memory cell function.", "contents": "Immunological memory function of the T and B cell types: distribution over mouse spleen and lymph nodes. Spleens from LAF1 mice injected intravenously with sheep erythrocytes (SE) are relatively rich in memory T cells early in the immune response (1 to 3 days) and rich in memory B cells as the response progresses (2 weeks or more). Marked cooperation for the secondary immune response in vitro was obtained by combining 10(6) spleen cells from LAF1 mice, taken 2 days after intravenous priming with SE, with 10(7) spleen cells from day 14 primed mice. The results indicate relative deficiencies in the spleen for B memory cells on days 1 to 2 and for T memory cells on day 14 after priming. Day -14, but not day -2, immune lymph node (LN) cells could replace the day -2 spleen cells (anti-Thy 1.2 sensitive) in the in vitro cooperation with day -14 immune spleen cells. Immune spleen cells taken 4 to 7 days after priming contain more equivalent numbers of B and T memory cells, but 10 to 7 days after transfer of such immune spleen cells without SE into irradiated recipients the T memory cells were again more prominent in lymph node and the B memory cells in spleen as shown by in vitro cooperation studies. These results suggest that during the second week after intravenous injection of SE relatively more T than B memory cells migrate from spleen to lymph node, resulting in an imbalance in the splenic memory cell population favoring B memory cell function."}
{"id": "PMID:1086226", "title": "Lack of requirement for thymocytes for efficient antibody formation to trinitrophenylated mouse red cells in mice: role for thymocytes in suppression of the immune response.", "content": "The requirement of thymus-derived cells for the immune response to trinitrophenylated syngeneic mouse red cells (TNP-MRC) was investigated. In three sets of experiments the following results were obtained: a) irradiated mice which were reconstituted with bone marrow cells alone showed a better anti-TNP response after injection with TNP-MRC than those reconstituted with both bone marrow cells and thymocytes. b) Anti-thymocyte serum augmented the mouse anti-TNP response to TNP-MRC. c) Nude thymusless mice showed a better anti-TNP response to TNP-MRC than their normal littermate controls. These results indicate that the anti-TNP response of mice to TNP-MRC does not require thymus-derived helper cells. Moreover, thymus-derived cells have a suppressive effect on the anti-TNP response.", "contents": "Lack of requirement for thymocytes for efficient antibody formation to trinitrophenylated mouse red cells in mice: role for thymocytes in suppression of the immune response. The requirement of thymus-derived cells for the immune response to trinitrophenylated syngeneic mouse red cells (TNP-MRC) was investigated. In three sets of experiments the following results were obtained: a) irradiated mice which were reconstituted with bone marrow cells alone showed a better anti-TNP response after injection with TNP-MRC than those reconstituted with both bone marrow cells and thymocytes. b) Anti-thymocyte serum augmented the mouse anti-TNP response to TNP-MRC. c) Nude thymusless mice showed a better anti-TNP response to TNP-MRC than their normal littermate controls. These results indicate that the anti-TNP response of mice to TNP-MRC does not require thymus-derived helper cells. Moreover, thymus-derived cells have a suppressive effect on the anti-TNP response."}
{"id": "PMID:1086227", "title": "Turnover of radioiodinated and of leucine-labeled immunoglobulin M in murine splenic lymphocytes.", "content": "Lactoperoxidase-catalyzed radioiodination of cell surface proteins and biosynthetic incorporation of tritiated leucine were used to study the size and turnover of cell-associated immunoglobulins in splenic lymphocytes of different size from BALB/c, C3H, and nu/nu mice. Small spleen cells, separated from large cells by velocity sedimentation, showed a single slow rate of turnover (t1/2 = 10-30 h) of surface radioiodinated or leucine-labeled IgM released as the monomeric 7-8 S subunit of IgM. Mitogen-activated large B cells and nonstimulated large spleen cells, separated by velocity sedimentation, released leucine-labeled IgM in an initial rapid phase (t1/2 = 2-4 h) as 19 S IgM, and later released 7-8 S IgM slowly (t1/2 = 10-30 h). The iodination reaction changed neither the biphasic mode of turnover, nor, even at higher concentration of H2O2, the size of the actively secreted 19 S IgM. Radioiodination of mitogen-activated large cells or of unstimulated large cells labeled only the slowly released 7-8 S IgM, not the actively secreted 19 S IgM. Large cells of nonstimulated spleen, however, released radioiodinated as well as leucine-labeled IgM also rapidly (t1/2 = 1-3 h). These rapidly released IgM molecules were found to be 7-8 S IgM subunits. Our results indicate that these turnover rate and size differences of radioiodinated and of leucine-labeled IgM released from murine spleen cells are in part resulting from the differences in the methodology of labeling and are in other parts due to different small and large splenic lymphocytes releasing IgM of different size at different rates.", "contents": "Turnover of radioiodinated and of leucine-labeled immunoglobulin M in murine splenic lymphocytes. Lactoperoxidase-catalyzed radioiodination of cell surface proteins and biosynthetic incorporation of tritiated leucine were used to study the size and turnover of cell-associated immunoglobulins in splenic lymphocytes of different size from BALB/c, C3H, and nu/nu mice. Small spleen cells, separated from large cells by velocity sedimentation, showed a single slow rate of turnover (t1/2 = 10-30 h) of surface radioiodinated or leucine-labeled IgM released as the monomeric 7-8 S subunit of IgM. Mitogen-activated large B cells and nonstimulated large spleen cells, separated by velocity sedimentation, released leucine-labeled IgM in an initial rapid phase (t1/2 = 2-4 h) as 19 S IgM, and later released 7-8 S IgM slowly (t1/2 = 10-30 h). The iodination reaction changed neither the biphasic mode of turnover, nor, even at higher concentration of H2O2, the size of the actively secreted 19 S IgM. Radioiodination of mitogen-activated large cells or of unstimulated large cells labeled only the slowly released 7-8 S IgM, not the actively secreted 19 S IgM. Large cells of nonstimulated spleen, however, released radioiodinated as well as leucine-labeled IgM also rapidly (t1/2 = 1-3 h). These rapidly released IgM molecules were found to be 7-8 S IgM subunits. Our results indicate that these turnover rate and size differences of radioiodinated and of leucine-labeled IgM released from murine spleen cells are in part resulting from the differences in the methodology of labeling and are in other parts due to different small and large splenic lymphocytes releasing IgM of different size at different rates."}
{"id": "PMID:1086228", "title": "Histocompatibility antigens acting as helper determinants for tumor-associated antigens of murine lymphosarcoma.", "content": "The mechanism of effectiveness of allogeneic versus syngeneic immunization in mounting a humoral specific immune response against tumor-associated antigens of murine urethan-induced thymic lymphosarcomas was studied. C57BL mice immunized with untreated or blocked C3Hf lymphoma cells yielded sera with complement-dependent cytotoxicity for C57BL lymphosarcomas, whereas they did not respond to repeated injections of blocked syngeneic lymphoma cells, indicating a helper activity of alien alloantigens in the reflex of tumor antigens. Thymectomized, lethally-irradiated and T, but not B-reconstituted mice produced active sera, suggesting a T-dependent response. The anti-tumor activity of the serum ran parallel with the anti-histocompatibility one. The tumor-associated and the alien histocompatibility determinants had to be present on the same cell membrane. In addition, active sera were obtained when immunizing syngeneic lymphoma cell inocula were followed by allogeneic normal immunocompetent cells. It is suggested that either a helper mechanism with B and T cells cooperating in recognizing haptenic tumor-associated and carrier histocompatibility antigens or a graft-versus-host reaction determining an abnormal induction of the immune system is needed in order to detect the weak tumor-associated antigens of urethan-induced lymphomas.", "contents": "Histocompatibility antigens acting as helper determinants for tumor-associated antigens of murine lymphosarcoma. The mechanism of effectiveness of allogeneic versus syngeneic immunization in mounting a humoral specific immune response against tumor-associated antigens of murine urethan-induced thymic lymphosarcomas was studied. C57BL mice immunized with untreated or blocked C3Hf lymphoma cells yielded sera with complement-dependent cytotoxicity for C57BL lymphosarcomas, whereas they did not respond to repeated injections of blocked syngeneic lymphoma cells, indicating a helper activity of alien alloantigens in the reflex of tumor antigens. Thymectomized, lethally-irradiated and T, but not B-reconstituted mice produced active sera, suggesting a T-dependent response. The anti-tumor activity of the serum ran parallel with the anti-histocompatibility one. The tumor-associated and the alien histocompatibility determinants had to be present on the same cell membrane. In addition, active sera were obtained when immunizing syngeneic lymphoma cell inocula were followed by allogeneic normal immunocompetent cells. It is suggested that either a helper mechanism with B and T cells cooperating in recognizing haptenic tumor-associated and carrier histocompatibility antigens or a graft-versus-host reaction determining an abnormal induction of the immune system is needed in order to detect the weak tumor-associated antigens of urethan-induced lymphomas."}
{"id": "PMID:1086229", "title": "Target cell-dependent T cell-mediated lysis of vaccinia virus-infected cells.", "content": "Vaccinia virus specific cytotoxicity against infected target cells was observed in vitro. Spleen lymphocytes from normal and immunized mice of the inbred strains C3H and DBA/2 were incubated with vaccinia virus-infected and non-infected 51 Cr-labeled mastocytoma P-815-X2 cells and L-929 fibroblasts, which were used as targets. Cytotoxic lymphocytes could be isolated from the mice as early as 2 days after infection with vaccinia virus. The highest cytotoxic effect was obtained with lymphocytes taken 6 days after infection. The degree of lysis was correlated with the ratio of immune lymphocytes to target cells. Specific blocking of target cell lysis resulted after addition of anti-vaccinia antibody from different sources. The effector cells could be characterized as T cells by elimination of macrophages and B cells. Target cell killing was only possible in a syngeneic system; allogeneic infected target cells were not lysed significantly.", "contents": "Target cell-dependent T cell-mediated lysis of vaccinia virus-infected cells. Vaccinia virus specific cytotoxicity against infected target cells was observed in vitro. Spleen lymphocytes from normal and immunized mice of the inbred strains C3H and DBA/2 were incubated with vaccinia virus-infected and non-infected 51 Cr-labeled mastocytoma P-815-X2 cells and L-929 fibroblasts, which were used as targets. Cytotoxic lymphocytes could be isolated from the mice as early as 2 days after infection with vaccinia virus. The highest cytotoxic effect was obtained with lymphocytes taken 6 days after infection. The degree of lysis was correlated with the ratio of immune lymphocytes to target cells. Specific blocking of target cell lysis resulted after addition of anti-vaccinia antibody from different sources. The effector cells could be characterized as T cells by elimination of macrophages and B cells. Target cell killing was only possible in a syngeneic system; allogeneic infected target cells were not lysed significantly."}
{"id": "PMID:1086230", "title": "Subclasses of T cells with different sensitivities to cytotoxic antibody in the presence of anesthetics.", "content": "When cytotoxic titrations were made of rabbit anti-mouse brain and anti-Thy-1.2 sera against mouse lymphoid cells in the presence of sodium barbital or xylocaine, it was observed that a proportion of T cells were much more sensitive (50 x) than the remainder. This effect could be detected with ethidium or fluorescein diacetate as indicators of cell death, but not with trypan blue or 51Cr. The highly sensitive cells were found in all lymphoid tissues, being most numerous in spleen and least in lymph nodes.", "contents": "Subclasses of T cells with different sensitivities to cytotoxic antibody in the presence of anesthetics. When cytotoxic titrations were made of rabbit anti-mouse brain and anti-Thy-1.2 sera against mouse lymphoid cells in the presence of sodium barbital or xylocaine, it was observed that a proportion of T cells were much more sensitive (50 x) than the remainder. This effect could be detected with ethidium or fluorescein diacetate as indicators of cell death, but not with trypan blue or 51Cr. The highly sensitive cells were found in all lymphoid tissues, being most numerous in spleen and least in lymph nodes."}
{"id": "PMID:1086231", "title": "p-Azobenzenearsonate-L-tyrosine-mediated helper function in immune responses of guinea pigs and rats.", "content": "A small bifunctional antigen (4-hydroxy-5-iodo-3-nitrophenyl)acetyl-epsilon-aminocaproyl-L-tyrosine-azobenzene-p-arsonate [NIP-cap-TYR(ABA)] was found to induce fair humoral antibody formation against NIP-cap but very little anti-ABA-TYR. This was observed in rats and guinea pigs. Prior immunization with ABA-TYR, either as such or coupled to dodecanoylated bovine serum albumin (lipid-BSA), primed rats for an enhanced anti-NIP response to NIP-cap-TYR(ABA). An attempt to encourage rats to produce anti-ABA-TYR in response to the bifunctional antigen by priming them with NIP-cap-lipid-BSA failed. Priming with ABA-TYR was dose-dependent. An injection of 1.5-15 nanomoles per rat primed for an increased production of anti-NIP while 150 nanomoles did not. Adult thymectomized x-irradiated rats had a poor anti-NIP response to the bifunctional antigen if they were reconstituted with T-enriched lymphoid cells from control mice, but a good response if reconstituted with similar cells from ABA-TYR-primed syngeneic rats.", "contents": "p-Azobenzenearsonate-L-tyrosine-mediated helper function in immune responses of guinea pigs and rats. A small bifunctional antigen (4-hydroxy-5-iodo-3-nitrophenyl)acetyl-epsilon-aminocaproyl-L-tyrosine-azobenzene-p-arsonate [NIP-cap-TYR(ABA)] was found to induce fair humoral antibody formation against NIP-cap but very little anti-ABA-TYR. This was observed in rats and guinea pigs. Prior immunization with ABA-TYR, either as such or coupled to dodecanoylated bovine serum albumin (lipid-BSA), primed rats for an enhanced anti-NIP response to NIP-cap-TYR(ABA). An attempt to encourage rats to produce anti-ABA-TYR in response to the bifunctional antigen by priming them with NIP-cap-lipid-BSA failed. Priming with ABA-TYR was dose-dependent. An injection of 1.5-15 nanomoles per rat primed for an increased production of anti-NIP while 150 nanomoles did not. Adult thymectomized x-irradiated rats had a poor anti-NIP response to the bifunctional antigen if they were reconstituted with T-enriched lymphoid cells from control mice, but a good response if reconstituted with similar cells from ABA-TYR-primed syngeneic rats."}
{"id": "PMID:1086232", "title": "Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes.", "content": "A modified technique was used to demonstrate lymphocytic acid alpha-naphthyl acetate esterase activity in frozen sections of mouse lymphoid tissue or smears. Positive, dot-like reaction products were noticed in more than 94% of the lymphocytes located in the diffuse cortical (\"paracortical\", \"thymus-dependent\") area of mesenteric lymph nodes of young adult ICR mice. Almost identical values were found for lymphocytes in the cisterna chyli. In contrast, the follicular cortex which is predominantly occupied by B cells, contained less than 7% esterase-positive lymphocytes. In vitro, cytotoxic anti-theta serum destroyed the vast majority of esterase positive lymphocytes while esterase-negative lymphocytes were resistent to this treatment. In mesenteric nodes of nude BALB/c (nu/nu) mice, follicular cortex and paracortex together contained approximately 16 times less esterase-positive lymphocytes than in the immunologically competent hybrid BALB/c (nu/+) animals. These findings indicate that non-specific acid esterase activity may serve as a criterion to differentiate peripheral T and B lymphocytes in lymph node sections and smears of mice by light microscopy. Possible implications of this enzyme activity in thymus-derived lymphocytes are discussed.", "contents": "Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. A modified technique was used to demonstrate lymphocytic acid alpha-naphthyl acetate esterase activity in frozen sections of mouse lymphoid tissue or smears. Positive, dot-like reaction products were noticed in more than 94% of the lymphocytes located in the diffuse cortical (\"paracortical\", \"thymus-dependent\") area of mesenteric lymph nodes of young adult ICR mice. Almost identical values were found for lymphocytes in the cisterna chyli. In contrast, the follicular cortex which is predominantly occupied by B cells, contained less than 7% esterase-positive lymphocytes. In vitro, cytotoxic anti-theta serum destroyed the vast majority of esterase positive lymphocytes while esterase-negative lymphocytes were resistent to this treatment. In mesenteric nodes of nude BALB/c (nu/nu) mice, follicular cortex and paracortex together contained approximately 16 times less esterase-positive lymphocytes than in the immunologically competent hybrid BALB/c (nu/+) animals. These findings indicate that non-specific acid esterase activity may serve as a criterion to differentiate peripheral T and B lymphocytes in lymph node sections and smears of mice by light microscopy. Possible implications of this enzyme activity in thymus-derived lymphocytes are discussed."}
{"id": "PMID:1086233", "title": "H-2-linked genetic control of antibody response to soluble calf skin collagen in mice.", "content": "An autosomal dominant immune response gene could be demonstrated in congenic resistant strains of mice which is linked to the H-2 locus and controls the antibody response to soluble calf collagen. High responsiveness was associated with the H-2 alleles, b and f, low responsiveness with the H-2 alleles, d, k, m and r. Studies with calf procollagen, which contains an additional carrier moiety, indicated that these genetic differences might be expressed at the level of T cells.", "contents": "H-2-linked genetic control of antibody response to soluble calf skin collagen in mice. An autosomal dominant immune response gene could be demonstrated in congenic resistant strains of mice which is linked to the H-2 locus and controls the antibody response to soluble calf collagen. High responsiveness was associated with the H-2 alleles, b and f, low responsiveness with the H-2 alleles, d, k, m and r. Studies with calf procollagen, which contains an additional carrier moiety, indicated that these genetic differences might be expressed at the level of T cells."}
{"id": "PMID:1086234", "title": "Regulation of the immune response by subclasses of T lymphocytes. I. Interactions between pre-killer T cells and regulatory T cells obtained from peripheral lymphoid tissues of mice.", "content": "The generation of killer cells to alloantigens in vitro depends upon an interaction between two subclasses of peripheral T cells: \"pre-killer\" T cells and \"regulatory\" T cells. The pre-killer T cell, found in highest concentrations in peripheral lymph nodes of mice, is sensitive in vivo to the administration of small doses of anti-thymocyte serum (ATS) and is depleted in vitro by treatment with (a) anti-Thy-1.2 + C, (b) low doses (600 r) of x-irradiation. Specificity of pre-killer cells is supported by adsorption of pre-killer activity on appropriate allogeneic monolayers and by the specific absence of this activity from lymphoid tissues of mice rendered tolerant in neonatal life. Although T cells remaining in the spleen 2 days after a small dose of ATS (SPA-T) did not generate killer cells, this subset exerted substantial regulatory effects upon the generation of killer activity by pre-killer T cells. The addition of this population of regulatory T cells to small numbers of lymph node T cells (LN-T) resulted in substantial enhancement in the generation of killer cells from the LN-T pre-killers. The addition of SPA-T to larger numbers of LN-T (which produce strong responses alone), failed to enhance the response, and in some cases resulted in significant suppression which could not easily be accounted for by alteration in cell numbers in culture. Although amplifier activity was relatively radioresistant, \"suppressor\" activity was sensitive to relatively small doses of irradiation. The significance of this T-T interaction is discussed.", "contents": "Regulation of the immune response by subclasses of T lymphocytes. I. Interactions between pre-killer T cells and regulatory T cells obtained from peripheral lymphoid tissues of mice. The generation of killer cells to alloantigens in vitro depends upon an interaction between two subclasses of peripheral T cells: \"pre-killer\" T cells and \"regulatory\" T cells. The pre-killer T cell, found in highest concentrations in peripheral lymph nodes of mice, is sensitive in vivo to the administration of small doses of anti-thymocyte serum (ATS) and is depleted in vitro by treatment with (a) anti-Thy-1.2 + C, (b) low doses (600 r) of x-irradiation. Specificity of pre-killer cells is supported by adsorption of pre-killer activity on appropriate allogeneic monolayers and by the specific absence of this activity from lymphoid tissues of mice rendered tolerant in neonatal life. Although T cells remaining in the spleen 2 days after a small dose of ATS (SPA-T) did not generate killer cells, this subset exerted substantial regulatory effects upon the generation of killer activity by pre-killer T cells. The addition of this population of regulatory T cells to small numbers of lymph node T cells (LN-T) resulted in substantial enhancement in the generation of killer cells from the LN-T pre-killers. The addition of SPA-T to larger numbers of LN-T (which produce strong responses alone), failed to enhance the response, and in some cases resulted in significant suppression which could not easily be accounted for by alteration in cell numbers in culture. Although amplifier activity was relatively radioresistant, \"suppressor\" activity was sensitive to relatively small doses of irradiation. The significance of this T-T interaction is discussed."}
{"id": "PMID:1086235", "title": "Regulation of the immune response by subclasses of T lymphocytes. II. The effect of adult thymectomy upon humoral and cellular responses in mice.", "content": "The immunologic consequences resulting from thymectomy in adult life were investigated. Primary humoral responses were not diminished shortly after adult thymectomy, as judged by responses of intact thymectomized mice as well as by the ability of spleen cells from such mice to transfer primary responses. However, secondary humoral responses were substantially reduced in irradiated recipients of spleen cells from primed adult-thymectomized mice, suggesting that the thymus is required in adult life to maintain a population of cells important in the generation of immunologic helper memory. By contrast, small doses of anti-thymocyte serum, which primarily affect recirulating T cells, abrogated the primary humoral response, but allowed the subsequent development of immune memory. Using a technique permitting in vitro sensitization of purified T cells to alloantigens, it was shown that adult thymectomy increases the ability of T cells to generate primary cytotoxic responses, but had little effect upon the development of cytotoxic T memory activity. These experiments suggest that in adult life the thymus maintains a regulatory population of T cells in peripheral tissues which suppress early T cell differentiation to cytotoxic effector cells and potentiates the development of immune memory.", "contents": "Regulation of the immune response by subclasses of T lymphocytes. II. The effect of adult thymectomy upon humoral and cellular responses in mice. The immunologic consequences resulting from thymectomy in adult life were investigated. Primary humoral responses were not diminished shortly after adult thymectomy, as judged by responses of intact thymectomized mice as well as by the ability of spleen cells from such mice to transfer primary responses. However, secondary humoral responses were substantially reduced in irradiated recipients of spleen cells from primed adult-thymectomized mice, suggesting that the thymus is required in adult life to maintain a population of cells important in the generation of immunologic helper memory. By contrast, small doses of anti-thymocyte serum, which primarily affect recirulating T cells, abrogated the primary humoral response, but allowed the subsequent development of immune memory. Using a technique permitting in vitro sensitization of purified T cells to alloantigens, it was shown that adult thymectomy increases the ability of T cells to generate primary cytotoxic responses, but had little effect upon the development of cytotoxic T memory activity. These experiments suggest that in adult life the thymus maintains a regulatory population of T cells in peripheral tissues which suppress early T cell differentiation to cytotoxic effector cells and potentiates the development of immune memory."}
{"id": "PMID:1086236", "title": "Antibody response to phosphorylcholine in vitro. I. Studies on the frequency of precursor cells, average clone size and cellular cooperation.", "content": "The anti-phosphorylcholine (PC) antibodies synthesized by BALB/c spleen cells in microcultures upon immunization with heat-killed vaccine of Pneumococci R36A (Pn) are directed exclusively to the PC epitope. These antibodies are of very restricted avidity and 88% of the responding clones express the idiotype characteristic of the TEPC-15 PC-binding myeloma. This idiotypic restriction appears to be due to the absence of clones capable of expressing other idiotypes, rather than to \"clonal dominance\". The estimated frequency of precursor cells for the PC epitope is 1 X 10(-5) to 2.5 X 10(-5). These precursors give rise to clones with an average size of 9 plaque-forming cells. When the logarithm of the number of negative wells was plotted against the number of spleen cells/well, the fraction of nonresponding cultures decreased exponentially as the number of spleen cells was increased. This indicated that only one cell type was limiting in our assay, presumably a B cell. Furthermore, treatment of spleen cells with AKR anti serum completely abolished the response to sheep red cells without affecting the response to PC. It is concluded that PC is a T cell-independent antigen. Of interest was the finding that PC requires adherent (A) cells and this is a particular characteristic of PC, since most T cell-independent antigens have been found not to require A cells. Reasons for the possible homogeneity of the response to PC are also discussed.", "contents": "Antibody response to phosphorylcholine in vitro. I. Studies on the frequency of precursor cells, average clone size and cellular cooperation. The anti-phosphorylcholine (PC) antibodies synthesized by BALB/c spleen cells in microcultures upon immunization with heat-killed vaccine of Pneumococci R36A (Pn) are directed exclusively to the PC epitope. These antibodies are of very restricted avidity and 88% of the responding clones express the idiotype characteristic of the TEPC-15 PC-binding myeloma. This idiotypic restriction appears to be due to the absence of clones capable of expressing other idiotypes, rather than to \"clonal dominance\". The estimated frequency of precursor cells for the PC epitope is 1 X 10(-5) to 2.5 X 10(-5). These precursors give rise to clones with an average size of 9 plaque-forming cells. When the logarithm of the number of negative wells was plotted against the number of spleen cells/well, the fraction of nonresponding cultures decreased exponentially as the number of spleen cells was increased. This indicated that only one cell type was limiting in our assay, presumably a B cell. Furthermore, treatment of spleen cells with AKR anti serum completely abolished the response to sheep red cells without affecting the response to PC. It is concluded that PC is a T cell-independent antigen. Of interest was the finding that PC requires adherent (A) cells and this is a particular characteristic of PC, since most T cell-independent antigens have been found not to require A cells. Reasons for the possible homogeneity of the response to PC are also discussed."}
{"id": "PMID:1086237", "title": "Recruitment of effector lymphocytes by initiator lymphocytes: role of a trypsin-sensitive membrane component.", "content": "In earlier studies we found that initiator T lymphocytes sensitized in vitro against fibroblasts were able to recruit immunospecific effector T lymphocytes in vivo in syngeneic mice. In the present study, to investigate the possible function in effector cell recruitment of sensitizing antigens passively adsorbed onto the initiator cell membrane, we treated the initiator lymphocytes with trypsin. We found that trypsin inhibited recruitment. However, the initiator cells spontaneously recovered their recruiting ability after incubation in vitro for 4 h in the absence of contact with sensitizing antigens. Recovery could be blocked by cycloheximide. Hence, it appears that recruitment depends on the intrinsic metabolic function of the initiator lymphocytes and is not due merely to passively-adsorbed antigen carried over into the recipient mouse.", "contents": "Recruitment of effector lymphocytes by initiator lymphocytes: role of a trypsin-sensitive membrane component. In earlier studies we found that initiator T lymphocytes sensitized in vitro against fibroblasts were able to recruit immunospecific effector T lymphocytes in vivo in syngeneic mice. In the present study, to investigate the possible function in effector cell recruitment of sensitizing antigens passively adsorbed onto the initiator cell membrane, we treated the initiator lymphocytes with trypsin. We found that trypsin inhibited recruitment. However, the initiator cells spontaneously recovered their recruiting ability after incubation in vitro for 4 h in the absence of contact with sensitizing antigens. Recovery could be blocked by cycloheximide. Hence, it appears that recruitment depends on the intrinsic metabolic function of the initiator lymphocytes and is not due merely to passively-adsorbed antigen carried over into the recipient mouse."}
{"id": "PMID:1086238", "title": "Immunoglobulin turnover in B lymphocyte subpopulations.", "content": "\"In vitro\" turnover of leucine-labeled and of radioiodinated IgM has been studied with cells from various lymphoid organs of nude mice, i.e. lymph nodes, thoracic duct, spleen and bone marrow, as well as with subpopulations of B cells from spleen and bone marrow separated by free flow electrophoresis. Three types of IgM-producing lymphocytes could be distinguished by their turnover rates of IgM, by the size of the released IgM and by the capacity of the IgM molecules to be labeled by the lactoperoxidase-catalyzed radioiodination reaction and/or by incorporation of radioactive leucine. Type I cells release 7-8 S IgM rapidly (t1/2 = 1-3h); the released IGM is leucine-labeled and radioiodinated. Type II cells release 7-8 S IgM slowly (t1/2 =10-30); the released IgM is leucine labeled and radioiodinated. Type III cells release 19 S IgM rapidly (t1/2 =2-4 h); the released IgM is leucine labeled, but not radioiodinated. Lymph nodes and thoracic duct contain predominantly type II cells, bone marrow contains type I and II cells, spleen contains type I,II and III cells. It is suggested that type III cells are Ig-secreting plaque-forming plasma cells, type II cells are small, resting \"memory\" B cells, and type I cells may be newly formed antigen-inexperienced B cells.", "contents": "Immunoglobulin turnover in B lymphocyte subpopulations. \"In vitro\" turnover of leucine-labeled and of radioiodinated IgM has been studied with cells from various lymphoid organs of nude mice, i.e. lymph nodes, thoracic duct, spleen and bone marrow, as well as with subpopulations of B cells from spleen and bone marrow separated by free flow electrophoresis. Three types of IgM-producing lymphocytes could be distinguished by their turnover rates of IgM, by the size of the released IgM and by the capacity of the IgM molecules to be labeled by the lactoperoxidase-catalyzed radioiodination reaction and/or by incorporation of radioactive leucine. Type I cells release 7-8 S IgM rapidly (t1/2 = 1-3h); the released IGM is leucine-labeled and radioiodinated. Type II cells release 7-8 S IgM slowly (t1/2 =10-30); the released IgM is leucine labeled and radioiodinated. Type III cells release 19 S IgM rapidly (t1/2 =2-4 h); the released IgM is leucine labeled, but not radioiodinated. Lymph nodes and thoracic duct contain predominantly type II cells, bone marrow contains type I and II cells, spleen contains type I,II and III cells. It is suggested that type III cells are Ig-secreting plaque-forming plasma cells, type II cells are small, resting \"memory\" B cells, and type I cells may be newly formed antigen-inexperienced B cells."}
{"id": "PMID:1086239", "title": "5-Hydroxytryptophan effect on the development of the immune response: IgM and IgG antibodies and rosette formation in primary and secondary responses.", "content": "In animals immunized with bovine serum albumin, 5-hydroxytryptophan prolonged the latent period of the IgM and IgG primary responses, decreased response intensity, delayed the response peak and suppressed IgG immunological memory. In 5-hydroxytryptophan-treated mice, the number of rosette-forming cells (RFC) in the lymph node and spleen decreased during the primary and secondary responses. This effect was due to the decreased number of IgG RFC and to the later involvement of IgM RFC in the immune response. The absence of the secondary response was related to unprimed IgG memory cells. 5-hydroxytryptophan does not inhibit the primary and secondary responses after the connections between the hypothalamus and pituitary have been disrupted. The participation of the n.raphe-hypothalaminc-pituitary system in immuno-regulation and the putative mechanism underlying serotonin effect on the immune response are discussed.", "contents": "5-Hydroxytryptophan effect on the development of the immune response: IgM and IgG antibodies and rosette formation in primary and secondary responses. In animals immunized with bovine serum albumin, 5-hydroxytryptophan prolonged the latent period of the IgM and IgG primary responses, decreased response intensity, delayed the response peak and suppressed IgG immunological memory. In 5-hydroxytryptophan-treated mice, the number of rosette-forming cells (RFC) in the lymph node and spleen decreased during the primary and secondary responses. This effect was due to the decreased number of IgG RFC and to the later involvement of IgM RFC in the immune response. The absence of the secondary response was related to unprimed IgG memory cells. 5-hydroxytryptophan does not inhibit the primary and secondary responses after the connections between the hypothalamus and pituitary have been disrupted. The participation of the n.raphe-hypothalaminc-pituitary system in immuno-regulation and the putative mechanism underlying serotonin effect on the immune response are discussed."}
{"id": "PMID:1086240", "title": "Hapten-induced B cell paralysis. II. Evidence for trivial mechanisms of tolerance.", "content": "Injection into mice of a free reactive form of the hapten (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP), induces a state of specific unresponsiveness to the hapten, upon its challenge with thymus-cependent and independent carriers. This unresponsiveness is maintained in vitro. Both induction and expression of the unresponsive state were found to be independent of T cells. Analysis of the mechanisms responsible for the B cell \"tolerance\" demonstrated that the major cause of unresponsiveness in this system is the blockade of specific surface receptors on B cells by the hapten, abolishing the focusing function of these receptors. \"Tolerant\" B cells, though they were unresponsive to the antigen, could be activated to anti-hapten antibody secretion by the B cell mitogen lipopolysaccharide (LPS), which does not require Ig-mediated binding. They also became fully responsive to the antigen NNP-LPS in specific thymus-independent responses after 24 hours of in vitro incubation in the absence of tolerogen, conditions which allow \"deblocking\" of Ig surface receptors, and, thus, restoration of the focusing function of these receptors. These results demonstrate that great care is required for a clearcut definition of B cell tolerance. In this example, the tolerant B cells were perfectly responsive to a competent ligand, and no indication of an altered triggering or effector processes was found. It appears that in this, as in many other cases of B cell tolerance, the systems or the animals are tolerant, whereas B cells maintain a resting nonactivated state and are fully responsive to the triggering signal. Thus, the existence of tolerance-inducing signals resulting in B cell unresponsiveness is questioned.", "contents": "Hapten-induced B cell paralysis. II. Evidence for trivial mechanisms of tolerance. Injection into mice of a free reactive form of the hapten (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP), induces a state of specific unresponsiveness to the hapten, upon its challenge with thymus-cependent and independent carriers. This unresponsiveness is maintained in vitro. Both induction and expression of the unresponsive state were found to be independent of T cells. Analysis of the mechanisms responsible for the B cell \"tolerance\" demonstrated that the major cause of unresponsiveness in this system is the blockade of specific surface receptors on B cells by the hapten, abolishing the focusing function of these receptors. \"Tolerant\" B cells, though they were unresponsive to the antigen, could be activated to anti-hapten antibody secretion by the B cell mitogen lipopolysaccharide (LPS), which does not require Ig-mediated binding. They also became fully responsive to the antigen NNP-LPS in specific thymus-independent responses after 24 hours of in vitro incubation in the absence of tolerogen, conditions which allow \"deblocking\" of Ig surface receptors, and, thus, restoration of the focusing function of these receptors. These results demonstrate that great care is required for a clearcut definition of B cell tolerance. In this example, the tolerant B cells were perfectly responsive to a competent ligand, and no indication of an altered triggering or effector processes was found. It appears that in this, as in many other cases of B cell tolerance, the systems or the animals are tolerant, whereas B cells maintain a resting nonactivated state and are fully responsive to the triggering signal. Thus, the existence of tolerance-inducing signals resulting in B cell unresponsiveness is questioned."}
{"id": "PMID:1086241", "title": "Immunological effects of IgT synthesized by theta-positive cell lines.", "content": "The effects of immunoglobulin (Ig) purified by affinity chromatography from two cultured T cell lines on in vitro immune responses was studied. IgT purified from both EL-4 and WEHI-22 suppressed the thymus-dependent IgM response, but had no effect on the thymus-independent IgM response. In contrast, the IgG responses to both thymus-dependent and independent antigens were augmented at optimal concentrations of IgT. Ig of B cell origin--serum Ig, myeloma Ig, B cell surface Ig -- did not have any of these effects. Because of the similarity of the effects of IgT purified from the lymphomas with the effects of T cells and their IgT, it seems likely that the IgT obtained from EL-4 and WEHI-22 cell lines are analogues of normal IgT and thus should be useful in elucidating some of the chemical and biological properties of IgT.", "contents": "Immunological effects of IgT synthesized by theta-positive cell lines. The effects of immunoglobulin (Ig) purified by affinity chromatography from two cultured T cell lines on in vitro immune responses was studied. IgT purified from both EL-4 and WEHI-22 suppressed the thymus-dependent IgM response, but had no effect on the thymus-independent IgM response. In contrast, the IgG responses to both thymus-dependent and independent antigens were augmented at optimal concentrations of IgT. Ig of B cell origin--serum Ig, myeloma Ig, B cell surface Ig -- did not have any of these effects. Because of the similarity of the effects of IgT purified from the lymphomas with the effects of T cells and their IgT, it seems likely that the IgT obtained from EL-4 and WEHI-22 cell lines are analogues of normal IgT and thus should be useful in elucidating some of the chemical and biological properties of IgT."}
{"id": "PMID:1086242", "title": "A simple rosette assay for demonstration of complement receptor sites using complement-coated zymosan beads.", "content": "In the present article we describe a simple rosette assay for detection of C3 receptor-bearing B lymphocytes with complement (C)-coated zymosan (Zy) beads. Zy coated with murine or human C bound to a distinct population of human and mouse lymphocytes as well as to the majority of lymphoblastoid cells of several human established cell lines. Rosette formation was also observed with human red blood cells, with human monocytes and neutrophils. Experiments with anti-immunoglobulin sera, with other B and T cell markers and with mouse thymocytes proved that the capacity to bind C-coated Zy is primarily a feature of B lymphocytes. The following findings suggested that C-coated Zy is bound via receptor sites for the activated components of C3: (a) Zy coated with C3-deficient serum failed to bind, (b) comparable percentages of various lymphoid and nonlymphoid cells formed rosettes with C-coated Zy as well as with antibody and C-coated sheep red blood cells, and (c) antibodies against human or murine C3 inhibited the binding of C-coated Zy.", "contents": "A simple rosette assay for demonstration of complement receptor sites using complement-coated zymosan beads. In the present article we describe a simple rosette assay for detection of C3 receptor-bearing B lymphocytes with complement (C)-coated zymosan (Zy) beads. Zy coated with murine or human C bound to a distinct population of human and mouse lymphocytes as well as to the majority of lymphoblastoid cells of several human established cell lines. Rosette formation was also observed with human red blood cells, with human monocytes and neutrophils. Experiments with anti-immunoglobulin sera, with other B and T cell markers and with mouse thymocytes proved that the capacity to bind C-coated Zy is primarily a feature of B lymphocytes. The following findings suggested that C-coated Zy is bound via receptor sites for the activated components of C3: (a) Zy coated with C3-deficient serum failed to bind, (b) comparable percentages of various lymphoid and nonlymphoid cells formed rosettes with C-coated Zy as well as with antibody and C-coated sheep red blood cells, and (c) antibodies against human or murine C3 inhibited the binding of C-coated Zy."}
{"id": "PMID:1086243", "title": "Ontogeny of the antibody-forming cell line in mice. I. Kinetics of appearance of mature B cells.", "content": "The development of functionally mature B cells in the liver and spleen of fetal and neonatal CBA mice has been followed. A mature B cell is defined as one giving rise to antibody-producing progeny within 2 days in culture. Very few such B cells are found during fetal life. After birth there is a rapid rise in the numbers reaching 1/3 of adult levels in the 7-day-old spleen.", "contents": "Ontogeny of the antibody-forming cell line in mice. I. Kinetics of appearance of mature B cells. The development of functionally mature B cells in the liver and spleen of fetal and neonatal CBA mice has been followed. A mature B cell is defined as one giving rise to antibody-producing progeny within 2 days in culture. Very few such B cells are found during fetal life. After birth there is a rapid rise in the numbers reaching 1/3 of adult levels in the 7-day-old spleen."}
{"id": "PMID:1086244", "title": "Suppression of immune response to sheep red blood cells in mice treated with preparations of a tumor cell component and in tumor-bearing mice.", "content": "The frequency of rosette-forming cells (RFC) in spleens of mice immunized with sheep red blood cells (SRBC) was significantly reduced by pretreatment with preparations obtained from tumor extracts. This effect was not produced by preparations obtained by similar methods from normal tissue extracts. Significant suppression of RFC frequency was also found in spleens of mice immunized with SRBC at late stages of tumor growth, as compared to that in immunized controls and in mice bearing tumors at early stages. The results suggest that at late stages, a tumor cell component is released by tumor cells which may be similar to the nondialyzable immune suppressive factor(s) separated by us from tumor extracts, factors found in ascites fluid of tumor-bearing mice and those known to be released from tumor cells in vitro.", "contents": "Suppression of immune response to sheep red blood cells in mice treated with preparations of a tumor cell component and in tumor-bearing mice. The frequency of rosette-forming cells (RFC) in spleens of mice immunized with sheep red blood cells (SRBC) was significantly reduced by pretreatment with preparations obtained from tumor extracts. This effect was not produced by preparations obtained by similar methods from normal tissue extracts. Significant suppression of RFC frequency was also found in spleens of mice immunized with SRBC at late stages of tumor growth, as compared to that in immunized controls and in mice bearing tumors at early stages. The results suggest that at late stages, a tumor cell component is released by tumor cells which may be similar to the nondialyzable immune suppressive factor(s) separated by us from tumor extracts, factors found in ascites fluid of tumor-bearing mice and those known to be released from tumor cells in vitro."}
{"id": "PMID:1086245", "title": "Persisting T cells in rats tolerant of human serum albumin. The significance of tolerant and nonimmune T cells which preferentially restrict high affinity antibody synthesis.", "content": "The adoptive response of primed rat thoracic duct lymphocytes ('TDL) following specific antigen challenge (soluble human serum albumin, s-HSA) was restricted when cells were transferred into syngeneic, adult (AS2 X AS)F1 hybrid recipients in comparison with irradiated hosts. This adoptive memory response was also inhibited in irradiated recipients by transferring nonimmune TDL along with 'TDL. Recirculating B cells (B-TDL) did not inhibit the 'TDL response, indicating that the adoptive secondary response was regulated by T cells. Antibody synthesis was preferentially restricted in the high affinity memory cell precursor population, demonstrating a role for T cells in regulating the maturation of antibody affinity. The adoptive memory response was liberated from this T regulatory effect in adult recipients when hosts were challenged with the alum-precipitated adjuvant form (HSA-adj) rather than the soluble form of HSA. Since the adoptive memory response was sensitive to the presence or absence of T cells, this experimental model was used to determine whether or not T cells were eliminated from HSA-tolerant rats. Antibody synthesis by 'TDL was reduced approximately 10-fold compared with controls when transferred into tolerant recipients and challenged with either s-HSA or HSA-adj; a similar reduction was not observed by substituting bovine serum albumin (BSA) 'TDL and challenging with s-BSA. The tolerance-induced inhibition of HSA 'TDL was destroyed by irradiation and TDL from HSA-tolerant donors were more effective than normal nonimmune TDL in reducing the adoptive HSA 'TDL response. HSA-tolerant TDL did not inhibit the BSA 'TDL response significantly. The results indicate that T cells are not eliminated by tolerance induction in this model and after interaction with tolerogen may exert an active (or competitive) role in restricting antibody synthesis by high affinity B memory cell precursors. However, the fact that tolerant T cells are not able to prevent a primary response suggests that unresponsiveness to HSA in the T compartment represents a functional deficiency and not an active suppression at this level. Nevertheless, the presence of these tolerant cells probably accounts for the failure of antibody affinity to mature in partially tolerant rats.", "contents": "Persisting T cells in rats tolerant of human serum albumin. The significance of tolerant and nonimmune T cells which preferentially restrict high affinity antibody synthesis. The adoptive response of primed rat thoracic duct lymphocytes ('TDL) following specific antigen challenge (soluble human serum albumin, s-HSA) was restricted when cells were transferred into syngeneic, adult (AS2 X AS)F1 hybrid recipients in comparison with irradiated hosts. This adoptive memory response was also inhibited in irradiated recipients by transferring nonimmune TDL along with 'TDL. Recirculating B cells (B-TDL) did not inhibit the 'TDL response, indicating that the adoptive secondary response was regulated by T cells. Antibody synthesis was preferentially restricted in the high affinity memory cell precursor population, demonstrating a role for T cells in regulating the maturation of antibody affinity. The adoptive memory response was liberated from this T regulatory effect in adult recipients when hosts were challenged with the alum-precipitated adjuvant form (HSA-adj) rather than the soluble form of HSA. Since the adoptive memory response was sensitive to the presence or absence of T cells, this experimental model was used to determine whether or not T cells were eliminated from HSA-tolerant rats. Antibody synthesis by 'TDL was reduced approximately 10-fold compared with controls when transferred into tolerant recipients and challenged with either s-HSA or HSA-adj; a similar reduction was not observed by substituting bovine serum albumin (BSA) 'TDL and challenging with s-BSA. The tolerance-induced inhibition of HSA 'TDL was destroyed by irradiation and TDL from HSA-tolerant donors were more effective than normal nonimmune TDL in reducing the adoptive HSA 'TDL response. HSA-tolerant TDL did not inhibit the BSA 'TDL response significantly. The results indicate that T cells are not eliminated by tolerance induction in this model and after interaction with tolerogen may exert an active (or competitive) role in restricting antibody synthesis by high affinity B memory cell precursors. However, the fact that tolerant T cells are not able to prevent a primary response suggests that unresponsiveness to HSA in the T compartment represents a functional deficiency and not an active suppression at this level. Nevertheless, the presence of these tolerant cells probably accounts for the failure of antibody affinity to mature in partially tolerant rats."}
{"id": "PMID:1086246", "title": "X-ray enhancement of splenic rosette-forming cells in nonimmune mice.", "content": "The formation of rosettes between spleen cells from nonimmune mice and sheep red blood cells (SRBC) has been investigated. The number of splenic rosette-forming cells (RFC) in the intact spleen was very low but greatly increased after sublethal whole body x-irradiation. These cells were inhibited by pretreatment of the cells with AKR anti-C3H- antigen antiserum, in the presence of fresh guinea pig serum, but not with rabbit anti-mouse immunoglobulin antiserum. The splenic RFC also increased after cortisone acetate administration into mice. These results suggest that the RFC are a T cell sub-population having x-ray resistant and cortisone-insensitive characteristics. The x-irradiation of mice in vivo selectively increased the number of splenic RFC in consequence of reduction in the number of cells having non-T cell characteristics. These RFC are presumably antigen-specific sheep red blood cell rosettes, a quite different situation as in the case of human T lymphocyte rosette formation.", "contents": "X-ray enhancement of splenic rosette-forming cells in nonimmune mice. The formation of rosettes between spleen cells from nonimmune mice and sheep red blood cells (SRBC) has been investigated. The number of splenic rosette-forming cells (RFC) in the intact spleen was very low but greatly increased after sublethal whole body x-irradiation. These cells were inhibited by pretreatment of the cells with AKR anti-C3H- antigen antiserum, in the presence of fresh guinea pig serum, but not with rabbit anti-mouse immunoglobulin antiserum. The splenic RFC also increased after cortisone acetate administration into mice. These results suggest that the RFC are a T cell sub-population having x-ray resistant and cortisone-insensitive characteristics. The x-irradiation of mice in vivo selectively increased the number of splenic RFC in consequence of reduction in the number of cells having non-T cell characteristics. These RFC are presumably antigen-specific sheep red blood cell rosettes, a quite different situation as in the case of human T lymphocyte rosette formation."}
{"id": "PMID:1086247", "title": "Analysis of the role of different cell types in the genetic regulation of antibody production to the thymus-independent synthetic polypeptide poly (DTyr, DGlu)-poly (DPro)--poly (DLys).", "content": "The immune response potential of mice to the thymus-independent synthetic polypeptide poly (DTyr, DGlu)-poly(DPro)--poly(DLys)[D(T,G)-Pro--L] is genetically regulated. The defect in the ability of low responder mice to mount an immune response to this antigen appears to be expressed in their B cell population since the presence of thymocytes, or addition of \"educated T cells\" or supernatant of T cells after stimulation with the antigen neither enhanced, nor suppressed the level of antibodies produced in both low and high responder mice. Low responsiveness could not be enhanced either by stimulation of macrophages or by injection of poly(A) - poly(U) in contrast to the significant effect of these agents on low responses to the thymus-dependent poly(LTyr, LGlu)-poly(LPro)--poly(LLys) [L(T,G)-Pro--L]. These results suggest that macrophages do not participate in the limiting step, or are not involved at all, in antibody production towards the thymus-independent polypeptide. The antibodies produced in response to D(T,G)-Pro--L were found to be mainly of the 7 S class. T cells are not required for the production of mercaptoethanol resistant antibodies to this immunogen since they were found in intact mice as well as in T cell depleted animals.", "contents": "Analysis of the role of different cell types in the genetic regulation of antibody production to the thymus-independent synthetic polypeptide poly (DTyr, DGlu)-poly (DPro)--poly (DLys). The immune response potential of mice to the thymus-independent synthetic polypeptide poly (DTyr, DGlu)-poly(DPro)--poly(DLys)[D(T,G)-Pro--L] is genetically regulated. The defect in the ability of low responder mice to mount an immune response to this antigen appears to be expressed in their B cell population since the presence of thymocytes, or addition of \"educated T cells\" or supernatant of T cells after stimulation with the antigen neither enhanced, nor suppressed the level of antibodies produced in both low and high responder mice. Low responsiveness could not be enhanced either by stimulation of macrophages or by injection of poly(A) - poly(U) in contrast to the significant effect of these agents on low responses to the thymus-dependent poly(LTyr, LGlu)-poly(LPro)--poly(LLys) [L(T,G)-Pro--L]. These results suggest that macrophages do not participate in the limiting step, or are not involved at all, in antibody production towards the thymus-independent polypeptide. The antibodies produced in response to D(T,G)-Pro--L were found to be mainly of the 7 S class. T cells are not required for the production of mercaptoethanol resistant antibodies to this immunogen since they were found in intact mice as well as in T cell depleted animals."}
{"id": "PMID:1086248", "title": "Role of epitope density in the induction of immunity and tolerance with thymus-independent antigens. II. Studies with 2,4-dinitrophenyl conjugates in vivo.", "content": "The immunogenicity and tolerogenicity of 2,4-dinitrophenyl (DNP) conjugates of levan, type 3 pneumococcal polysaccharide (SIII), dextran B512 and dextran B 1299 with different levels of substitution were assessed in vivo. It was confirmed that, as in vitro, conjugates with low epitope density are solely immunogenic for Bmu cells, whereas higher density conjugates are both immunogenic and tolerogenic according to dosage. The maximal anti-DNP plaque-forming cell responses attainable varied by 2 log10 according to the carrier, being greatest with dextrans and least with SIII. The effect of the carrier on immunogenicity was not attributable to B cells responding to it, as concurrent tolerization by either free polysaccharide or the conjugate itself was without effect. Optimal immunization was succeeded by varying degrees of responsiveness (\"exhaustion\") which was greatest with the more immunogenic conjugates. In contrast, reduction of tolerizing dose by cyclophosphamide suppression was only a feature of more highly substituted conjugates.", "contents": "Role of epitope density in the induction of immunity and tolerance with thymus-independent antigens. II. Studies with 2,4-dinitrophenyl conjugates in vivo. The immunogenicity and tolerogenicity of 2,4-dinitrophenyl (DNP) conjugates of levan, type 3 pneumococcal polysaccharide (SIII), dextran B512 and dextran B 1299 with different levels of substitution were assessed in vivo. It was confirmed that, as in vitro, conjugates with low epitope density are solely immunogenic for Bmu cells, whereas higher density conjugates are both immunogenic and tolerogenic according to dosage. The maximal anti-DNP plaque-forming cell responses attainable varied by 2 log10 according to the carrier, being greatest with dextrans and least with SIII. The effect of the carrier on immunogenicity was not attributable to B cells responding to it, as concurrent tolerization by either free polysaccharide or the conjugate itself was without effect. Optimal immunization was succeeded by varying degrees of responsiveness (\"exhaustion\") which was greatest with the more immunogenic conjugates. In contrast, reduction of tolerizing dose by cyclophosphamide suppression was only a feature of more highly substituted conjugates."}
{"id": "PMID:1086249", "title": "The effect of the thymus-independent antigens, collagen and synthetic collagen-like polypeptide, on the requirement of cell cooperation in the immune response to thymus-dependent antigens.", "content": "The effect of thymus-independent antigens on the need for cell cooperation in the immune response to thymus-dependent antigens was investigated. Irradiated recipient mice transplanted with either bone marrow cells or a mixture of bone marrow and thymus cells, were immunized with the thymus-independent antigen (Pro-Gly-Pro)n covalently conjugated to the thymus-dependent ovalbumin, or with a mixture of (Pro-Gly-Pro)n and ovalbumin. In both cases an effective response towards ovalbumin was observed in the absence of thymus cells as was found for the thymus-independent (Pro-Gly-Pro)n. The same effect on ovalbumin was demonstrated when a mixture of the thymus-independent collagen and ovalbumin was used for immunization. On the other hand, when irradiated reconstituted mice were immunized with a mixture of ovalbumin and the thymus-dependent gelatin, which is the denatured product of collagen, cell-to-cell cooperation was required for an immune response to both immunogens. The effect of (Pro-Gly-Pro)n and collagen on the response to the thymus-dependent ovalbumin in vivo was observed in in vitro experiments using sheep red blood cells (SRBC) as the immunogen as well. In the presence of reduced and carboxymethylated (RCM) Ascaris collagen and (Pro-Gly-Pro)n, nude spleen cells could produce significant numbers of plaque-forming cells towards SRBC. Thus, (Pro-Gly-Pro)n and collagen can deliver the signal required to stimulate B cells to produce antibody towards thymus-dependent antigens in the absence of T cells. In contrast to the results with (Pro-Gly-Pro)n and collagen, the thymus-independent synthetic polypeptide poly(DTyr, DGlu-)-poly(DPro)--poly(DLys) did not affect the requirement for cell cooperation of the thymus-dependent immunogens, ovalbumin and SRCB. It thus appears that the ability to substitute for T cells for antibody production towards thymus-dependent immunogens is not a general characteristic of thymus-independent antigens.", "contents": "The effect of the thymus-independent antigens, collagen and synthetic collagen-like polypeptide, on the requirement of cell cooperation in the immune response to thymus-dependent antigens. The effect of thymus-independent antigens on the need for cell cooperation in the immune response to thymus-dependent antigens was investigated. Irradiated recipient mice transplanted with either bone marrow cells or a mixture of bone marrow and thymus cells, were immunized with the thymus-independent antigen (Pro-Gly-Pro)n covalently conjugated to the thymus-dependent ovalbumin, or with a mixture of (Pro-Gly-Pro)n and ovalbumin. In both cases an effective response towards ovalbumin was observed in the absence of thymus cells as was found for the thymus-independent (Pro-Gly-Pro)n. The same effect on ovalbumin was demonstrated when a mixture of the thymus-independent collagen and ovalbumin was used for immunization. On the other hand, when irradiated reconstituted mice were immunized with a mixture of ovalbumin and the thymus-dependent gelatin, which is the denatured product of collagen, cell-to-cell cooperation was required for an immune response to both immunogens. The effect of (Pro-Gly-Pro)n and collagen on the response to the thymus-dependent ovalbumin in vivo was observed in in vitro experiments using sheep red blood cells (SRBC) as the immunogen as well. In the presence of reduced and carboxymethylated (RCM) Ascaris collagen and (Pro-Gly-Pro)n, nude spleen cells could produce significant numbers of plaque-forming cells towards SRBC. Thus, (Pro-Gly-Pro)n and collagen can deliver the signal required to stimulate B cells to produce antibody towards thymus-dependent antigens in the absence of T cells. In contrast to the results with (Pro-Gly-Pro)n and collagen, the thymus-independent synthetic polypeptide poly(DTyr, DGlu-)-poly(DPro)--poly(DLys) did not affect the requirement for cell cooperation of the thymus-dependent immunogens, ovalbumin and SRCB. It thus appears that the ability to substitute for T cells for antibody production towards thymus-dependent immunogens is not a general characteristic of thymus-independent antigens."}
{"id": "PMID:1086250", "title": "Expression of a receptor for IgM by human T cells in vitro.", "content": "The capacity of human peripheral blood lymphocytes (PBL) to bind antigen-IgG antibody (EA(IgG)) and antigen-IgM antibody (EA(IgM)) complexes was investigated using a rosette technique with ox erythrocytes coated with rabbit IgG or IgM antibody. It was found that while EA(IgG)-rosette-forming cells (RFC) were detected on PBL freshly drawn from normal individuals, EA(IgM)-RFC were present only in suspensions kept in culture for 24 h in medi\u00e1 supplemented with sera containing very low or no amounts of IgM. Experiments of simultaneous detection of EA(IgG)-RFC or EA(IgM)-RFC and other membrane markers for human T or B cells together with experiments on purified T or B cell populations indicated that EA(IgG)-RFC were formed by both T and B cells, while T cells only were capable of EA(IgM) rosette formation. The specificity of the receptors for IgG and IgM was determined by studying the inhibitory capacity of purified human IgM and IgG in the rosette assay. The receptor for IgG was inhibited by IgG and not by IgM, while the reverse was true for the receptor for IgM.", "contents": "Expression of a receptor for IgM by human T cells in vitro. The capacity of human peripheral blood lymphocytes (PBL) to bind antigen-IgG antibody (EA(IgG)) and antigen-IgM antibody (EA(IgM)) complexes was investigated using a rosette technique with ox erythrocytes coated with rabbit IgG or IgM antibody. It was found that while EA(IgG)-rosette-forming cells (RFC) were detected on PBL freshly drawn from normal individuals, EA(IgM)-RFC were present only in suspensions kept in culture for 24 h in medi\u00e1 supplemented with sera containing very low or no amounts of IgM. Experiments of simultaneous detection of EA(IgG)-RFC or EA(IgM)-RFC and other membrane markers for human T or B cells together with experiments on purified T or B cell populations indicated that EA(IgG)-RFC were formed by both T and B cells, while T cells only were capable of EA(IgM) rosette formation. The specificity of the receptors for IgG and IgM was determined by studying the inhibitory capacity of purified human IgM and IgG in the rosette assay. The receptor for IgG was inhibited by IgG and not by IgM, while the reverse was true for the receptor for IgM."}
{"id": "PMID:1086263", "title": "Studies on the subpopulation and function of peripheral lymphocytes, and inhibitor to PHA stimulation existing in the serum of patients with liver disease.", "content": "In our recent report we observed the abnormal response to PHA stimulation in the peripheral lymphocytes of patients with chronic liver diseases. The present report concerns extensive studies of this earlier report; analysis of the subpopulation and function of peripheral lymphocytes in patients with liver diseases. PHA responsiveness in acute hepatitis was reduced in the acute phase and improved during the convalescent stage. In chronic liver disease, half of the patients showed a decrease in T-cell population as well as a reduced response to PHA stimulation. These phenomena were observed more frequently in HBs-antigen positive patients than in HBs-antigen negative cases. Furthermore, we found the existence of an inhibitor to PHA response in the serum of 8 cases among 19 patients with various liver diseases. In patients with such a serum inhibitor, the reactivity of the lymphocyte itself to PHA was considerably higher but the T-cell population was much more decreased than that of inhibitor negative cases.", "contents": "Studies on the subpopulation and function of peripheral lymphocytes, and inhibitor to PHA stimulation existing in the serum of patients with liver disease. In our recent report we observed the abnormal response to PHA stimulation in the peripheral lymphocytes of patients with chronic liver diseases. The present report concerns extensive studies of this earlier report; analysis of the subpopulation and function of peripheral lymphocytes in patients with liver diseases. PHA responsiveness in acute hepatitis was reduced in the acute phase and improved during the convalescent stage. In chronic liver disease, half of the patients showed a decrease in T-cell population as well as a reduced response to PHA stimulation. These phenomena were observed more frequently in HBs-antigen positive patients than in HBs-antigen negative cases. Furthermore, we found the existence of an inhibitor to PHA response in the serum of 8 cases among 19 patients with various liver diseases. In patients with such a serum inhibitor, the reactivity of the lymphocyte itself to PHA was considerably higher but the T-cell population was much more decreased than that of inhibitor negative cases."}
{"id": "PMID:1086264", "title": "Case report: a bleeding Meckel's diverticulum diagnosed by selective arteriography.", "content": "The diagnosis of a bleeding Meckel's diverticulum was made by angiography in a 71 year old man who presented with melena and hemorrhagic shock. Emergent angiographic examination revealed an extravasation of contrast media from the ileal artery, which suggested a bleeding from the Meckel's diverticulum. The case illustrates the utility of selective angiography in case of gastrointestinal hemorrhage, where the lesion is beyond the range endoscopy.", "contents": "Case report: a bleeding Meckel's diverticulum diagnosed by selective arteriography. The diagnosis of a bleeding Meckel's diverticulum was made by angiography in a 71 year old man who presented with melena and hemorrhagic shock. Emergent angiographic examination revealed an extravasation of contrast media from the ileal artery, which suggested a bleeding from the Meckel's diverticulum. The case illustrates the utility of selective angiography in case of gastrointestinal hemorrhage, where the lesion is beyond the range endoscopy."}
{"id": "PMID:1086265", "title": "Comparative, immunological studies on lymphangiectasia of the small intestine revealed in protein losing gastroenteropathy and Beh\u00e7et's disease.", "content": "Lymphangiectasia of the small intestine was demonstrated in 9 to 18 cases with protein losing gastroenteropathy and in 10 of 26 cases with Beh\u00e7het's disease. Protein losing gastroenteropathy was compared to Beh\u00e7et's disease in view of immunological aspects. Immunoglobulin containing cells in the jejunal mucosa of protein losing gastroenteropathy were decreased, whereas Beh\u00e7et's disease had normal or increased distribution. This suggested that suggested that immunoglobulin may be lost into the intestinal lumen or that production of those cells may be inhibited in protein losing gastroenteropathy. Decreased DNCB skin reaction and impaired blastoid transformation of peripheral lymphocytes in both of two diseases suggested that there should be immunological defect in those diseases. However, in Beh\u00e7et's disease, investigation of the thymus disclosed hyperplasia and the presence of lymphoid follicle which does not appear in normal state. In conclusion, protein losing gastroenteropathy is in immunological deficiency state due to the congenital or acquired disorders of lymphatics which implies abnormal protein loss, decreased immunoglobulin containing cells in the jejunal mucosa and decreased sensitivity. On the contrary, in Beh\u00e7et's disease hyperimmune state is present based on thymic hyperplasia.", "contents": "Comparative, immunological studies on lymphangiectasia of the small intestine revealed in protein losing gastroenteropathy and Beh\u00e7et's disease. Lymphangiectasia of the small intestine was demonstrated in 9 to 18 cases with protein losing gastroenteropathy and in 10 of 26 cases with Beh\u00e7het's disease. Protein losing gastroenteropathy was compared to Beh\u00e7et's disease in view of immunological aspects. Immunoglobulin containing cells in the jejunal mucosa of protein losing gastroenteropathy were decreased, whereas Beh\u00e7et's disease had normal or increased distribution. This suggested that suggested that immunoglobulin may be lost into the intestinal lumen or that production of those cells may be inhibited in protein losing gastroenteropathy. Decreased DNCB skin reaction and impaired blastoid transformation of peripheral lymphocytes in both of two diseases suggested that there should be immunological defect in those diseases. However, in Beh\u00e7et's disease, investigation of the thymus disclosed hyperplasia and the presence of lymphoid follicle which does not appear in normal state. In conclusion, protein losing gastroenteropathy is in immunological deficiency state due to the congenital or acquired disorders of lymphatics which implies abnormal protein loss, decreased immunoglobulin containing cells in the jejunal mucosa and decreased sensitivity. On the contrary, in Beh\u00e7et's disease hyperimmune state is present based on thymic hyperplasia."}
{"id": "PMID:1086266", "title": "How to detect and treat arrhythmias in chronic lung disease.", "content": "Investigators suspect that arrhythmias are behind the high incidence of sudden death in patients with severe chronic bronchitis and emphysema. Continuous electrocardiographic monitoring may help boost survival statistics, since it has repeatedly shown its ability to detect irregularities that standard ECGs miss. So-called benign arrhythmias, such as atrial fibrillation, frequent ventricular premature contractions, and atrial flutter, are anything but harmless. In patients whose heart and lung function is already impaired by severe airway obstruction, these rhythm disorders can rapidly progress to a fatal cardiac arrest. Deciding how to treat the arrhythmia depends on the type of disturbance and the patient's clinical status. Direct-current shock or antiarrhythmic drugs can turn the tide in a desperately ill patient with acute respiratory failure. Correction of coexisting metabolic and ventilatory disorders often is crucial to successful therapy.", "contents": "How to detect and treat arrhythmias in chronic lung disease. Investigators suspect that arrhythmias are behind the high incidence of sudden death in patients with severe chronic bronchitis and emphysema. Continuous electrocardiographic monitoring may help boost survival statistics, since it has repeatedly shown its ability to detect irregularities that standard ECGs miss. So-called benign arrhythmias, such as atrial fibrillation, frequent ventricular premature contractions, and atrial flutter, are anything but harmless. In patients whose heart and lung function is already impaired by severe airway obstruction, these rhythm disorders can rapidly progress to a fatal cardiac arrest. Deciding how to treat the arrhythmia depends on the type of disturbance and the patient's clinical status. Direct-current shock or antiarrhythmic drugs can turn the tide in a desperately ill patient with acute respiratory failure. Correction of coexisting metabolic and ventilatory disorders often is crucial to successful therapy."}
{"id": "PMID:1086268", "title": "Ischaemic colitis in the experimental animal. I. Comparison of the effects of acute and subacute vascular occlusion.", "content": "Acute ligation of the common colic and caudal mesenteric artery in the dog led to ischaemic colitis, whereas the acute ligation of the common colic artery and gradual occlusion of the caudal mesenteric artery by an ameroid plastic device did not lead to ischaemic disease. These findings suggest that the collateral blood vessels do not open up rapidly enough to prevent ischaemic change when the two major arteries are acutely ligated.", "contents": "Ischaemic colitis in the experimental animal. I. Comparison of the effects of acute and subacute vascular occlusion. Acute ligation of the common colic and caudal mesenteric artery in the dog led to ischaemic colitis, whereas the acute ligation of the common colic artery and gradual occlusion of the caudal mesenteric artery by an ameroid plastic device did not lead to ischaemic disease. These findings suggest that the collateral blood vessels do not open up rapidly enough to prevent ischaemic change when the two major arteries are acutely ligated."}
{"id": "PMID:1086269", "title": "Peroral small-intestinal biopsy: experience with the hydraulic multiple biopsy instrument in routine clinical practice.", "content": "Experience of the peroral, hydraulic, multiple, small-bowel biopsy instrument is recorded and compared with reported experience of other peroral biopsy instruments. It is concluded that, in routine clinical practice, there is no particular danger associated with this instrument despite warnings to the contrary. Furthermore, biopsies are obtained at least as quickly as with other instruments and with great reliability. Since this instrument also enables multiple, precisely located biopsies to be taken from various levels of the small intestine, it could be considered the instrument of choice for peroral jejunal biopsy.", "contents": "Peroral small-intestinal biopsy: experience with the hydraulic multiple biopsy instrument in routine clinical practice. Experience of the peroral, hydraulic, multiple, small-bowel biopsy instrument is recorded and compared with reported experience of other peroral biopsy instruments. It is concluded that, in routine clinical practice, there is no particular danger associated with this instrument despite warnings to the contrary. Furthermore, biopsies are obtained at least as quickly as with other instruments and with great reliability. Since this instrument also enables multiple, precisely located biopsies to be taken from various levels of the small intestine, it could be considered the instrument of choice for peroral jejunal biopsy."}
{"id": "PMID:1086282", "title": "Radioimmunoassay of secretin in plasma.", "content": "A sensitive, specific and reproducible radioimmunoassay for secretin is described. Antibodies were readily produced against low microgram quantities of synthetic secretin. The secretin antibodies did not cross-react with the structurally similar G.I.P., V.I.P., or glucagon. Synthetic secretin was iodinated using Chloramine \"T\" and purified by a two-state procedure incorporating gel filtration and radient elution from a cation exchange column. Plasma samples were found to produce variable interference in the assay necessitating the incorporation of secretin-free \"blands\" for each patient's plasma. Production of secretin-free plasma was by incubation of plasma samples at 37 degrees C for 96 hours. The sensitivity of the assay was 12.5-25 pg/ml. Normal fasting secretin levels were 21 +/- S.E. 7 pg/ml. A mean rise in plasma secretin to 220 pg/ml was observed after intraduodenal acidification.", "contents": "Radioimmunoassay of secretin in plasma. A sensitive, specific and reproducible radioimmunoassay for secretin is described. Antibodies were readily produced against low microgram quantities of synthetic secretin. The secretin antibodies did not cross-react with the structurally similar G.I.P., V.I.P., or glucagon. Synthetic secretin was iodinated using Chloramine \"T\" and purified by a two-state procedure incorporating gel filtration and radient elution from a cation exchange column. Plasma samples were found to produce variable interference in the assay necessitating the incorporation of secretin-free \"blands\" for each patient's plasma. Production of secretin-free plasma was by incubation of plasma samples at 37 degrees C for 96 hours. The sensitivity of the assay was 12.5-25 pg/ml. Normal fasting secretin levels were 21 +/- S.E. 7 pg/ml. A mean rise in plasma secretin to 220 pg/ml was observed after intraduodenal acidification."}
{"id": "PMID:1086283", "title": "Genetic variants of serum alpha1-antitrypsin (Pi types) in Portuguese.", "content": "The results of Pi typing on 330 Portuguese from the area of Lisbon are reported. We found six phenotypes and four alleles out of the 24 described in the literature. The allele PiM is the most frequent as in other populations, PiS shows a high frequency (0.1152), and PiF is absent, which agrees satisfactorily with former studies carried out in Spain. These results are compared with others and the entity of the Iberian population is evoked.", "contents": "Genetic variants of serum alpha1-antitrypsin (Pi types) in Portuguese. The results of Pi typing on 330 Portuguese from the area of Lisbon are reported. We found six phenotypes and four alleles out of the 24 described in the literature. The allele PiM is the most frequent as in other populations, PiS shows a high frequency (0.1152), and PiF is absent, which agrees satisfactorily with former studies carried out in Spain. These results are compared with others and the entity of the Iberian population is evoked."}
{"id": "PMID:1086284", "title": "Clonal dominance and the preservation of clonal memory cells mediated by antigen-antibody.", "content": "Selected B-cell clones and their well characterized monoclonal antibody products were used to analyse the role of antibody in clonal dominance and the regulation of memory cell supplies. The experimental design was to permit contact between spleen cells and antigen in vitro, and to administer antibody to DNP prior to or following cell transfers into irradiated recipients. The anti-hapten response was strongly suppressed by the clone's own antibody or higher affinity antibody administered on day 0. Antigen-antibody inhibited memory cell generation. The suppressive effect was temporary, and reversible with time and further antigen and the same clone could be induced to produce antibody again, analysed by isoelectric focusing. We were therefore not dealing with clonal deletion. Change in the source of clonal anti-hapten excluded possible effects of antibody to carrier protein or idiotypic determinants in this system. The timing of antibody administration indicates that clones already triggered in the first 4 days after antigen contact could not be suppressed by antibody. Passive antibody to DNP only suppressed when both B and T cells had been permitted contact with hapten-carrier protein. Alteration of the carrier protein enabled us to study the effect of antigen-antibody on B and T cells separately. B cells binding antigen and antibody to hapten were triggered more efficiently by fresh T cells recognizing the carrier protein than after antigen uptake alone. Antibody to DNP suppressed only when both B and T cells had taken up hapten-protein, suggesting that antigen-antibody acts centrally at the level of both B memory cells and T helper cells. This reversible antigen-antibody blockade appears to favour the preservation of a pool of long-lived memory cells rather than the priming of new clones developing from short lived precursor cells; clonal dominance ensues.", "contents": "Clonal dominance and the preservation of clonal memory cells mediated by antigen-antibody. Selected B-cell clones and their well characterized monoclonal antibody products were used to analyse the role of antibody in clonal dominance and the regulation of memory cell supplies. The experimental design was to permit contact between spleen cells and antigen in vitro, and to administer antibody to DNP prior to or following cell transfers into irradiated recipients. The anti-hapten response was strongly suppressed by the clone's own antibody or higher affinity antibody administered on day 0. Antigen-antibody inhibited memory cell generation. The suppressive effect was temporary, and reversible with time and further antigen and the same clone could be induced to produce antibody again, analysed by isoelectric focusing. We were therefore not dealing with clonal deletion. Change in the source of clonal anti-hapten excluded possible effects of antibody to carrier protein or idiotypic determinants in this system. The timing of antibody administration indicates that clones already triggered in the first 4 days after antigen contact could not be suppressed by antibody. Passive antibody to DNP only suppressed when both B and T cells had been permitted contact with hapten-carrier protein. Alteration of the carrier protein enabled us to study the effect of antigen-antibody on B and T cells separately. B cells binding antigen and antibody to hapten were triggered more efficiently by fresh T cells recognizing the carrier protein than after antigen uptake alone. Antibody to DNP suppressed only when both B and T cells had taken up hapten-protein, suggesting that antigen-antibody acts centrally at the level of both B memory cells and T helper cells. This reversible antigen-antibody blockade appears to favour the preservation of a pool of long-lived memory cells rather than the priming of new clones developing from short lived precursor cells; clonal dominance ensues."}
{"id": "PMID:1086281", "title": "Endocardial lesions following coronary artery by-pass surgery.", "content": "A series of post-operative deaths is presented in which coronary artery by-pass surgery had been performed at various ante-mortem intervals by the same surgical term in a single institution. When compared to an appropriate control population, these cases displayed a sequence of endocardial inflammation and progressive fibro-elastosis which suggested that injury had occurred during surgery. An assessment of the effects of this lesion and of possible etiologic factors is presented.", "contents": "Endocardial lesions following coronary artery by-pass surgery. A series of post-operative deaths is presented in which coronary artery by-pass surgery had been performed at various ante-mortem intervals by the same surgical term in a single institution. When compared to an appropriate control population, these cases displayed a sequence of endocardial inflammation and progressive fibro-elastosis which suggested that injury had occurred during surgery. An assessment of the effects of this lesion and of possible etiologic factors is presented."}
{"id": "PMID:1086285", "title": "Lymphocyte responses to phytohaemagglutinin: age-related effects.", "content": "Cell-mediated immunity is depressed in elderly individuals compared to young individuals, and lymphocytes from elderly individuals have been reported to have impaired lymphocyte responsiveness to stimulation by PHA after 4 days of culture. We have confirmed this observation. However, after 8 days of culture, the lymphocyte responses were greater in elderly normal individuals than in young normal individuals. Responses of lymphocytes from young individuals decreased with time from 4 to 8 days in culture, while there were increased responses with time when lymphocytes from elderly individuals were studied. When adherent cells from lymphocytes of young individuals were removed by  passage through protein-coated Degalan-bead columns, the lymphocyte responses to PHA were significantly increased at 8 days. Passage of lymphocytes from elderly individuals through coated Degalan bead columns did not alter the lymphocyte responses. Removal of macrophages from the mononuclear cells obtained from young individuals did not result in increased lymphocyte responses to PHA after 8 days in culture. Removal of adherent cells appeared to have the same effect regardless of the efficiency of removing B cells. The adherent cells removed by the protein coated columns, therefore, appear to be nonphagocytic mononuclear cells which are not B lymphocytes.", "contents": "Lymphocyte responses to phytohaemagglutinin: age-related effects. Cell-mediated immunity is depressed in elderly individuals compared to young individuals, and lymphocytes from elderly individuals have been reported to have impaired lymphocyte responsiveness to stimulation by PHA after 4 days of culture. We have confirmed this observation. However, after 8 days of culture, the lymphocyte responses were greater in elderly normal individuals than in young normal individuals. Responses of lymphocytes from young individuals decreased with time from 4 to 8 days in culture, while there were increased responses with time when lymphocytes from elderly individuals were studied. When adherent cells from lymphocytes of young individuals were removed by  passage through protein-coated Degalan-bead columns, the lymphocyte responses to PHA were significantly increased at 8 days. Passage of lymphocytes from elderly individuals through coated Degalan bead columns did not alter the lymphocyte responses. Removal of macrophages from the mononuclear cells obtained from young individuals did not result in increased lymphocyte responses to PHA after 8 days in culture. Removal of adherent cells appeared to have the same effect regardless of the efficiency of removing B cells. The adherent cells removed by the protein coated columns, therefore, appear to be nonphagocytic mononuclear cells which are not B lymphocytes."}
{"id": "PMID:1086286", "title": "The enhancement of the haemolytic activity of the first component of complement by trasylol.", "content": "Trasylol, a basic polypeptide extracted from bovine lung considerably enhances the complement activity of whole serum. The complement component influenced by Trasylol is C1, either in its activated form (C1) or in its precursor form (C1). An effect on the binding of C1 to the immunoglobulin molecule has been excluded and on becoming part of a cellular intermediate C1 has been shown to be insensitive to Trasylol. It seems that Trasylol modulates C1 in such a way as to render it more efficient haemolytically. An attempt has been made to explain these results by consideration of the relationship existing between complement and other biological systems, such as the fibrinolytic and kallikrein-kinin systems.", "contents": "The enhancement of the haemolytic activity of the first component of complement by trasylol. Trasylol, a basic polypeptide extracted from bovine lung considerably enhances the complement activity of whole serum. The complement component influenced by Trasylol is C1, either in its activated form (C1) or in its precursor form (C1). An effect on the binding of C1 to the immunoglobulin molecule has been excluded and on becoming part of a cellular intermediate C1 has been shown to be insensitive to Trasylol. It seems that Trasylol modulates C1 in such a way as to render it more efficient haemolytically. An attempt has been made to explain these results by consideration of the relationship existing between complement and other biological systems, such as the fibrinolytic and kallikrein-kinin systems."}
{"id": "PMID:1086287", "title": "Autoreactivity developing spontaneously in cultured mouse spleen cells. III. Inhibition of anti-embryo cytotoxicity in male T lymphocytes by female non-T cells.", "content": "Cultured female spleen cells develop less cytotoxicity to embryo-associated antigens than do cultured male spleen cells. This difference is less pronounced if the cells are treated before culture with a specific hetero-anti-B cell antiserum. Female non-T cells (especially from primiparous animals) can decrease the spontaneously developing T-cell cytotoxicity in male splenic T lymphocytes upon culture. By artificially manipulating the T cell: non-T cell ratio in the spleen population prior to culture, it seems one can alter the type of cytotoxic mechanism (to embryo-associated antigens) which develops in the cultures.", "contents": "Autoreactivity developing spontaneously in cultured mouse spleen cells. III. Inhibition of anti-embryo cytotoxicity in male T lymphocytes by female non-T cells. Cultured female spleen cells develop less cytotoxicity to embryo-associated antigens than do cultured male spleen cells. This difference is less pronounced if the cells are treated before culture with a specific hetero-anti-B cell antiserum. Female non-T cells (especially from primiparous animals) can decrease the spontaneously developing T-cell cytotoxicity in male splenic T lymphocytes upon culture. By artificially manipulating the T cell: non-T cell ratio in the spleen population prior to culture, it seems one can alter the type of cytotoxic mechanism (to embryo-associated antigens) which develops in the cultures."}
{"id": "PMID:1086288", "title": "T-lymphocyte number and function and the course of hepatitis B in hemodialysis patients.", "content": "To study the relation between general cellular immunity and the course of hepatitis B within a group of chronic hemodialysis patients, T-lymphocyte number and function were investigated in 13 persistently hepatitis B surface antigen (HBSAg)-positive patients, in 32 HBSAg-negative patients, and in 11 patients who had recovered from hepatitis B and compared with that of 21 age-matched controls. Phytohemagglutinin-induced lymphocyte stimulation in vitro and the number of circulating T-cells in the HBSAg-positive group were significantly decreased as compared with those of the recovered group and the controls. Lymphocyte stimulation by pokeweed mitogen and by an antigen cocktail showed the same tendency, but no significant differences between the HBSAg-positive and the recovered groups. Uremic sera from the three patient groups had similar depressive effects on phytohemagglutinin induced lymphocyte stimulation of controls. Serum immunoglobulin G, (IgG), IgA, and IgM were normal in the three patient groups. It is concluded that chronic hemodialysis patients, who have become persistent HBSAg-carriers, have a significantly decreased T-lymphocyte number and function as compared with hemodialysis patients who are able to eliminat hepatitis B virus (HBV). The difference could not be ascribed to the HBV infection itself. This indicates that T-cells play an important role in the elimination of HBV in hemodialysis patients.", "contents": "T-lymphocyte number and function and the course of hepatitis B in hemodialysis patients. To study the relation between general cellular immunity and the course of hepatitis B within a group of chronic hemodialysis patients, T-lymphocyte number and function were investigated in 13 persistently hepatitis B surface antigen (HBSAg)-positive patients, in 32 HBSAg-negative patients, and in 11 patients who had recovered from hepatitis B and compared with that of 21 age-matched controls. Phytohemagglutinin-induced lymphocyte stimulation in vitro and the number of circulating T-cells in the HBSAg-positive group were significantly decreased as compared with those of the recovered group and the controls. Lymphocyte stimulation by pokeweed mitogen and by an antigen cocktail showed the same tendency, but no significant differences between the HBSAg-positive and the recovered groups. Uremic sera from the three patient groups had similar depressive effects on phytohemagglutinin induced lymphocyte stimulation of controls. Serum immunoglobulin G, (IgG), IgA, and IgM were normal in the three patient groups. It is concluded that chronic hemodialysis patients, who have become persistent HBSAg-carriers, have a significantly decreased T-lymphocyte number and function as compared with hemodialysis patients who are able to eliminat hepatitis B virus (HBV). The difference could not be ascribed to the HBV infection itself. This indicates that T-cells play an important role in the elimination of HBV in hemodialysis patients."}
{"id": "PMID:1086289", "title": "Adoptive transfer of variant-specific resistance to Trypanosoma rhodesiense with B lymphocytes and serum.", "content": "Mice survive an otherwise lethal infection with Trypanosoma rhodesiense if previously inoculated with irradiated organisms. This resistance demonstrates immunological specificity, since even animals inoculated with irradiated trypanosomes were killed when challenged with trypanosomes of a nonhomologous or variant antigenic type. This resistance could be transferred to syngeneic recipients with serum or B lymphocytes. T lymphocytes had no capacity to transfer resistance. Although mice were protected from active infection on challenge at least 25 days after inoculation of irradiated organisms, this resistance could be transferred with spleen cells for only 10 days after immunization. Resistance could be transferred with serum for a minimum of 25 days after immunization. These studies implicate an antibody-mediated mechanism as having a major role in resistance to T. rhodesiense infections.", "contents": "Adoptive transfer of variant-specific resistance to Trypanosoma rhodesiense with B lymphocytes and serum. Mice survive an otherwise lethal infection with Trypanosoma rhodesiense if previously inoculated with irradiated organisms. This resistance demonstrates immunological specificity, since even animals inoculated with irradiated trypanosomes were killed when challenged with trypanosomes of a nonhomologous or variant antigenic type. This resistance could be transferred to syngeneic recipients with serum or B lymphocytes. T lymphocytes had no capacity to transfer resistance. Although mice were protected from active infection on challenge at least 25 days after inoculation of irradiated organisms, this resistance could be transferred with spleen cells for only 10 days after immunization. Resistance could be transferred with serum for a minimum of 25 days after immunization. These studies implicate an antibody-mediated mechanism as having a major role in resistance to T. rhodesiense infections."}
{"id": "PMID:1086290", "title": "Gamma radiolysis of thymine in oxygen-free aqueous solution in the presence of electron affinic radiosensitizers: identification of stable products.", "content": "Radiosensitizers react with nucleic radicals by addition and by electron transfer reactions. We report the steady-state gamma radiolysis of 1 mM thymine in oxygen-free aqueous solutions containing different classes of radiosensitizing drugs: N-oxyl-free radicals (TAN and TMPN), quinones (menadione and naphtoquinone), nitroheterocyclic compounds (metronidazole and 5-nitro-2-furoic acid) and N-ethylmaleimide. Two classes of thymine degradation products were isolated by thin-layer chromatography and characterized by spectroscopic measurements. The main products, irrespective of radiosensitizers, resulting from oxidation reaction were identified as the cis and trans isomers of 5,6-dihydroxy-5,6-dihydrothymine, N-pyruvyl-N'-formylurea, 6-hydroxy-5,6-dihydrothymine and 5-hydroxy-5,6-dihydrothymine. In the experimental conditions used only N-oxyls and to a lesser extent NEM reacted with 5-hydroxy-5,6-dihydrothymin-6-yl radical, giving stable covalently-bonded addition products with a high yield. TAN showed a higher binding ability with respect to TMPN, which is in good agreement with the rate-constants previously reported for these bimolecular reactions.", "contents": "Gamma radiolysis of thymine in oxygen-free aqueous solution in the presence of electron affinic radiosensitizers: identification of stable products. Radiosensitizers react with nucleic radicals by addition and by electron transfer reactions. We report the steady-state gamma radiolysis of 1 mM thymine in oxygen-free aqueous solutions containing different classes of radiosensitizing drugs: N-oxyl-free radicals (TAN and TMPN), quinones (menadione and naphtoquinone), nitroheterocyclic compounds (metronidazole and 5-nitro-2-furoic acid) and N-ethylmaleimide. Two classes of thymine degradation products were isolated by thin-layer chromatography and characterized by spectroscopic measurements. The main products, irrespective of radiosensitizers, resulting from oxidation reaction were identified as the cis and trans isomers of 5,6-dihydroxy-5,6-dihydrothymine, N-pyruvyl-N'-formylurea, 6-hydroxy-5,6-dihydrothymine and 5-hydroxy-5,6-dihydrothymine. In the experimental conditions used only N-oxyls and to a lesser extent NEM reacted with 5-hydroxy-5,6-dihydrothymin-6-yl radical, giving stable covalently-bonded addition products with a high yield. TAN showed a higher binding ability with respect to TMPN, which is in good agreement with the rate-constants previously reported for these bimolecular reactions."}
{"id": "PMID:1086291", "title": "On the radiation chemistry of some pyrimidine-electron adducts: reaction of pyrimidine negative ions with t-butanol radicals.", "content": "The rates of decay of the electron adducts of thymine, thymidylic acid and of uracil at pH approximately 8-5 and at wave-lengths of 340 or 350 nm in the presence of t-butanol as scavenger for OH radicals are not influenced significantly by the addition of 0-1 M NaClO4. It follows that the decay reactions do not take place between negatively-charged species. The best explanation seems to be an efficient reaction of the electron adducts with the t-butanol radicals formed by reaction of OH radicals with the t-butanol.", "contents": "On the radiation chemistry of some pyrimidine-electron adducts: reaction of pyrimidine negative ions with t-butanol radicals. The rates of decay of the electron adducts of thymine, thymidylic acid and of uracil at pH approximately 8-5 and at wave-lengths of 340 or 350 nm in the presence of t-butanol as scavenger for OH radicals are not influenced significantly by the addition of 0-1 M NaClO4. It follows that the decay reactions do not take place between negatively-charged species. The best explanation seems to be an efficient reaction of the electron adducts with the t-butanol radicals formed by reaction of OH radicals with the t-butanol."}
{"id": "PMID:1086292", "title": "DNA replication and post-replication repair in U.V.-sensitive mouse neuroblastoma cells.", "content": "Mouse neuroblastoma cells differentiate when grown in the absence of serum; differentiation is reversed on the addition of serum. Differentiated cells are more sensitive to U.V.-radiation than proliferating cells. Whereas addition of serum to differentiated neuroblastoma cells normally results in immediate, synchronous entry into S phase, irradiation just before the addition of serum results in a long delay in the onset of DNA replication. During this lag period, incorporated 3H-thymidine appears in the light density region of CsCl gradientss, reflecting either repair synthesis or abortive replication. Post-replication repair (gap-filling) was found to be present in proliferating cells and at certain times in differentiated cells. It is suggested that the sensitivity of differentiated neuroblastoma cells to U.V.-radiation may be due to ineffective post-replication repair or to deficiencies in more than one repair mechanism, with reduction in repair capacity beyond a critical threshold.", "contents": "DNA replication and post-replication repair in U.V.-sensitive mouse neuroblastoma cells. Mouse neuroblastoma cells differentiate when grown in the absence of serum; differentiation is reversed on the addition of serum. Differentiated cells are more sensitive to U.V.-radiation than proliferating cells. Whereas addition of serum to differentiated neuroblastoma cells normally results in immediate, synchronous entry into S phase, irradiation just before the addition of serum results in a long delay in the onset of DNA replication. During this lag period, incorporated 3H-thymidine appears in the light density region of CsCl gradientss, reflecting either repair synthesis or abortive replication. Post-replication repair (gap-filling) was found to be present in proliferating cells and at certain times in differentiated cells. It is suggested that the sensitivity of differentiated neuroblastoma cells to U.V.-radiation may be due to ineffective post-replication repair or to deficiencies in more than one repair mechanism, with reduction in repair capacity beyond a critical threshold."}
{"id": "PMID:1086293", "title": "In vitro morphologic transformation of Syrian hamster cells by U.V.-irradiation is enhanced by X-irridation and unaffected by chemical carcinogens.", "content": "U.V.-exposure of foetal hamster cells (secondary or tertiary sub-cultures) seeded for colony formation resulted in the induction of transformation. The U.V.-effect on colony-forming ability resulted in a D37 of 58 ergs/mm2. The observed transformation frequency induced by U.V.-increases approximately in proportion to increased doses in the range of 7-5-60-8 ergs/mm2 examined. When cells seeded for colony formation were treated with U.V. and later with either benzo(a)pyrene or N-acetoxy-fluorenyl-acetamide, commencing 6 min after exposure to U.V., there was neither an additive nor a synergistic enhancement of the transformation frequency ordinarily observed with either chemical agent alone. In mass cultures that were X-irradiated with 250 R, seeded for colony formation and treated with either 15 or 30 ergs/mm2 of U.V. 48 hours later, the enhancement of transformation was approximately 12- and 6-fold on a colony basis and 6- and 3-fold on a dish basis, respectively. No transformation occurred after X-irradiation only. The enhancement obtained by the pre-treatment with X-ray is imilar to that reported for the combination of X-ray and chemical carcinogens.", "contents": "In vitro morphologic transformation of Syrian hamster cells by U.V.-irradiation is enhanced by X-irridation and unaffected by chemical carcinogens. U.V.-exposure of foetal hamster cells (secondary or tertiary sub-cultures) seeded for colony formation resulted in the induction of transformation. The U.V.-effect on colony-forming ability resulted in a D37 of 58 ergs/mm2. The observed transformation frequency induced by U.V.-increases approximately in proportion to increased doses in the range of 7-5-60-8 ergs/mm2 examined. When cells seeded for colony formation were treated with U.V. and later with either benzo(a)pyrene or N-acetoxy-fluorenyl-acetamide, commencing 6 min after exposure to U.V., there was neither an additive nor a synergistic enhancement of the transformation frequency ordinarily observed with either chemical agent alone. In mass cultures that were X-irradiated with 250 R, seeded for colony formation and treated with either 15 or 30 ergs/mm2 of U.V. 48 hours later, the enhancement of transformation was approximately 12- and 6-fold on a colony basis and 6- and 3-fold on a dish basis, respectively. No transformation occurred after X-irradiation only. The enhancement obtained by the pre-treatment with X-ray is imilar to that reported for the combination of X-ray and chemical carcinogens."}
{"id": "PMID:1086294", "title": "DNA strand breaking and rejoining in response to ultraviolet light in normal human and xeroderma pigmentosum cells.", "content": "We describe a reproducible technique for measuring DNA strand breaking and rejoining in cells after treatment with U.V.-light. Results obtained with normal human cells, xeroderma pigmentosum cells (XP, complementation group A) and XP variant cells suggest that all three of these cell-types can carry out single-strand incision with equal rapidity. However, the breaks so induced appeared to be only slowly rejoined in the XP variant cells and rejoined not at all in XP complementation group A cells. Furthermore, parental strand rejoining was inhibited by caffeine in XP variant cells but not in normal cells.", "contents": "DNA strand breaking and rejoining in response to ultraviolet light in normal human and xeroderma pigmentosum cells. We describe a reproducible technique for measuring DNA strand breaking and rejoining in cells after treatment with U.V.-light. Results obtained with normal human cells, xeroderma pigmentosum cells (XP, complementation group A) and XP variant cells suggest that all three of these cell-types can carry out single-strand incision with equal rapidity. However, the breaks so induced appeared to be only slowly rejoined in the XP variant cells and rejoined not at all in XP complementation group A cells. Furthermore, parental strand rejoining was inhibited by caffeine in XP variant cells but not in normal cells."}
{"id": "PMID:1086297", "title": "Deficit in DNA content relative to histones in X-irradiated HeLa cells.", "content": "The DNA and histone content of HeLa S-3 cell cultures was measured by direct mass assays 21 hours after 1000 rad of X-irradiation, when the cells were arrested in G2 phase. The nuclear DNA content of such cultures was found to be deficient (73% of control values). In contrast, the synthesis of nuclear histones persisted, and the total histone content was close to 100% of control values. When synchronously-growing cultures were irradiated in mid-S phase and examined 3-5 hours later in G2 phase, both DNA and histone content were equal to control values.", "contents": "Deficit in DNA content relative to histones in X-irradiated HeLa cells. The DNA and histone content of HeLa S-3 cell cultures was measured by direct mass assays 21 hours after 1000 rad of X-irradiation, when the cells were arrested in G2 phase. The nuclear DNA content of such cultures was found to be deficient (73% of control values). In contrast, the synthesis of nuclear histones persisted, and the total histone content was close to 100% of control values. When synchronously-growing cultures were irradiated in mid-S phase and examined 3-5 hours later in G2 phase, both DNA and histone content were equal to control values."}
{"id": "PMID:1086298", "title": "Modification of radiation-induced oxic and anoxic damage by caffeine and potassium permanganate in barley seeds.", "content": "We show that both the immediate and post-irradiation oxygen effects in barley seeds decrease in magnitude in the presence of potassium permanganate and caffeine. This implies that these two types of oxygen effect have features in common. With the removal of the radiation-induced oxygen-sensitive sites, by anoxic hydration, caffeine potentiates the oxygen-independent component of damage, in seeds irradiated in a dry or pre-soaked state. Potassium permangenate, on the other hand, enhances the anoxic radiation damage only in seeds irradiated in a dry state. The possible mode of action of KMnO4 and caffeine in barley seeds is discussed.", "contents": "Modification of radiation-induced oxic and anoxic damage by caffeine and potassium permanganate in barley seeds. We show that both the immediate and post-irradiation oxygen effects in barley seeds decrease in magnitude in the presence of potassium permanganate and caffeine. This implies that these two types of oxygen effect have features in common. With the removal of the radiation-induced oxygen-sensitive sites, by anoxic hydration, caffeine potentiates the oxygen-independent component of damage, in seeds irradiated in a dry or pre-soaked state. Potassium permangenate, on the other hand, enhances the anoxic radiation damage only in seeds irradiated in a dry state. The possible mode of action of KMnO4 and caffeine in barley seeds is discussed."}
{"id": "PMID:1086299", "title": "Radiation effect on the proliferating capillaries in rat transparent chambers.", "content": "The revascularization of the devascularized area in rat transparent chambers by the proliferating blood vessels was studied quantitatively on the changes of vascularity. On the vascularizing border, a hypervascular zone about 0-5 mm wide was formed. The border advanced constantly at 0-020 cm a day. Vascular density was fixed in the repaired area. The vascular length and repaired area increased on parabolic curves. The chambers were irradiated with single doses of 50, 100, 200, 400, 1000, 2000, and 4000 rad of 30Co gamma-rays, and the effects on the advance of the border were examined. With 200 rad and more, significant inhibition, greater with bigger doses, was seen in the advance of the borders. Regression of the borders also occurred. Irradiation with over 1000 rad destroyed the hypervascular zones.", "contents": "Radiation effect on the proliferating capillaries in rat transparent chambers. The revascularization of the devascularized area in rat transparent chambers by the proliferating blood vessels was studied quantitatively on the changes of vascularity. On the vascularizing border, a hypervascular zone about 0-5 mm wide was formed. The border advanced constantly at 0-020 cm a day. Vascular density was fixed in the repaired area. The vascular length and repaired area increased on parabolic curves. The chambers were irradiated with single doses of 50, 100, 200, 400, 1000, 2000, and 4000 rad of 30Co gamma-rays, and the effects on the advance of the border were examined. With 200 rad and more, significant inhibition, greater with bigger doses, was seen in the advance of the borders. Regression of the borders also occurred. Irradiation with over 1000 rad destroyed the hypervascular zones."}
{"id": "PMID:1086301", "title": "Defective immunocompetence in foetal undernutrition.", "content": "The rate of Ig-bearing lymphocytes (B-cells) and of rosette-forming cells (T-cells) in peripheral blood as well as the lymphocyte response to phytohaemagglutinine (PHA) stimulation were evaluated in 19 small-for-date infants in order to assess both humoral and cellular immunity. A significant decrease of Ig-positive cells and of E-rosettes was found, compared with new-borns of appropriate birth weight, However, the response to PHA stimulation appeared to be impaired in only 3 of the observed cases.", "contents": "Defective immunocompetence in foetal undernutrition. The rate of Ig-bearing lymphocytes (B-cells) and of rosette-forming cells (T-cells) in peripheral blood as well as the lymphocyte response to phytohaemagglutinine (PHA) stimulation were evaluated in 19 small-for-date infants in order to assess both humoral and cellular immunity. A significant decrease of Ig-positive cells and of E-rosettes was found, compared with new-borns of appropriate birth weight, However, the response to PHA stimulation appeared to be impaired in only 3 of the observed cases."}
{"id": "PMID:1086303", "title": "Effect of adenosine 5'-triphosphate-dependent deoxyribonuclease deficiency on properties and transformation of Haemophilus influenzae strains.", "content": "A transformation-deficient strain of Haemophilus influenzae, lacking adenosine 5'-triphosphate-dependent deoxyribonuclease activity, was isolated by selection for sensitivity to mitomycin. The mutant, designated JK57, possibily showed a moderate sensitivity to ultraviolet (UV) irradiation and treatment with methyl methane sulfonate. Contrary to the wild type, the mutant degraded chromosomal deoxyribonucleic acid (DNA) to some extent. However, after UV irradiation to the mutant degraded considerably less DNA than the wild type and the TD24 mutant of H. influenzae, the latter being equivalent to a recA mutant of Escherichia coli. A TD2457 double mutant, constructed by transferring the TD24 mutation into the JK57 strain, was as sensitive to deleterious agents and as deficient in transformation as the TD24 single mutant; in the double mutant, however, after UV irradiation chromosomal DNA was degraded to the same extent as in the JK57 mutant. The number of transformants per unit of radioactive donor DNA taken up by JK57 recipient cells was approximately 10-fold smaller than in the wild type. Presynaptically, the fate of donor DNA in the adenosine 5'-triphosphate-dependent deoxyribonuclease-deficient mutants was not different from that in the wild type. In contrast to TD24 and the TD2457 double mutant, in the JK57 mutant, recombinant-type activities (molecules carrying both the donor and recipient markers) were formed almost as well as in the wild type. After integration into the JK57 recipient genome, the rate of replication of the donor marker was equal to that of the recipient marker during a number of generations, which suggests that the donor DNA is normally integrated into the JK57 chromosome. It is suggested that transformed JK57 cells pass with a high frequency into a type of cells that can replicate their chromosomes many times but have lost the ability to form visible colonies after plating.", "contents": "Effect of adenosine 5'-triphosphate-dependent deoxyribonuclease deficiency on properties and transformation of Haemophilus influenzae strains. A transformation-deficient strain of Haemophilus influenzae, lacking adenosine 5'-triphosphate-dependent deoxyribonuclease activity, was isolated by selection for sensitivity to mitomycin. The mutant, designated JK57, possibily showed a moderate sensitivity to ultraviolet (UV) irradiation and treatment with methyl methane sulfonate. Contrary to the wild type, the mutant degraded chromosomal deoxyribonucleic acid (DNA) to some extent. However, after UV irradiation to the mutant degraded considerably less DNA than the wild type and the TD24 mutant of H. influenzae, the latter being equivalent to a recA mutant of Escherichia coli. A TD2457 double mutant, constructed by transferring the TD24 mutation into the JK57 strain, was as sensitive to deleterious agents and as deficient in transformation as the TD24 single mutant; in the double mutant, however, after UV irradiation chromosomal DNA was degraded to the same extent as in the JK57 mutant. The number of transformants per unit of radioactive donor DNA taken up by JK57 recipient cells was approximately 10-fold smaller than in the wild type. Presynaptically, the fate of donor DNA in the adenosine 5'-triphosphate-dependent deoxyribonuclease-deficient mutants was not different from that in the wild type. In contrast to TD24 and the TD2457 double mutant, in the JK57 mutant, recombinant-type activities (molecules carrying both the donor and recipient markers) were formed almost as well as in the wild type. After integration into the JK57 recipient genome, the rate of replication of the donor marker was equal to that of the recipient marker during a number of generations, which suggests that the donor DNA is normally integrated into the JK57 chromosome. It is suggested that transformed JK57 cells pass with a high frequency into a type of cells that can replicate their chromosomes many times but have lost the ability to form visible colonies after plating."}
{"id": "PMID:1086304", "title": "Incorporation of tritium into cell materials of Rhodopseudomonas spheroides from tritiated water in the medium under aerobic conditions.", "content": "When Rhodopseudomonas spheroides cells grown aerobically in the dark were incubated in medium containing tritiated water (THO), incorporation of T into the bacterial cell materials occurred under growth and no-growth conditions. The overall T incorporation under no-growth conditions was stimulated by vigorous aeration and was suppressed strongly in the presence of either 10(-3) M KCN or 0.3% HgCl2, indicating that the bulk of the incorporation might depend upon bacterial cell metabolism or respiration. 10 mug/ml chloramphenicol and 20 mug/ml rifamipicin slightly suppressed the T incorporation. The extent of T incorporation was proportional to the concentration of T in the medium. Accordingly, regardless of differences in the concentration of T in the medium, the maximum ratio of T content per hydrogen atom in the cell materials to that of THO in the medium was approximately 0.2 in non-growing cells and 0.5 in growing cells, whereas the value was 0.02-0.03 in cells incubated in medium containing KCN or HgCl2. The non-growing cells aerated in THO medium were lyophilized and fractionated by the modified method of Schneider. More than 40% of the total T incorporated into the cell materials was recovered in the cold PCA-soluble fraction, whereas the distribution of T into fractions solbule in ether-ethanol, hot PCA and alkali was 10 to 20% each. More than 75% of the T extracted in the cold PCA-soluble fraction was volatile. While the amounts of RNA and protein in the non-growing cells decreased on adding chloramphenicol or rifampicin, the distribution of T in these fractions did not change much. Our results on T incorporation into non-growing cells indicate that the major T incorporation into bacterial cell materials is independent of biosynthetic reactions using labeled precursors produced by the assimilation of T into metabolites, but presumably depends on energy-linked conformational changes of macromolecules.", "contents": "Incorporation of tritium into cell materials of Rhodopseudomonas spheroides from tritiated water in the medium under aerobic conditions. When Rhodopseudomonas spheroides cells grown aerobically in the dark were incubated in medium containing tritiated water (THO), incorporation of T into the bacterial cell materials occurred under growth and no-growth conditions. The overall T incorporation under no-growth conditions was stimulated by vigorous aeration and was suppressed strongly in the presence of either 10(-3) M KCN or 0.3% HgCl2, indicating that the bulk of the incorporation might depend upon bacterial cell metabolism or respiration. 10 mug/ml chloramphenicol and 20 mug/ml rifamipicin slightly suppressed the T incorporation. The extent of T incorporation was proportional to the concentration of T in the medium. Accordingly, regardless of differences in the concentration of T in the medium, the maximum ratio of T content per hydrogen atom in the cell materials to that of THO in the medium was approximately 0.2 in non-growing cells and 0.5 in growing cells, whereas the value was 0.02-0.03 in cells incubated in medium containing KCN or HgCl2. The non-growing cells aerated in THO medium were lyophilized and fractionated by the modified method of Schneider. More than 40% of the total T incorporated into the cell materials was recovered in the cold PCA-soluble fraction, whereas the distribution of T into fractions solbule in ether-ethanol, hot PCA and alkali was 10 to 20% each. More than 75% of the T extracted in the cold PCA-soluble fraction was volatile. While the amounts of RNA and protein in the non-growing cells decreased on adding chloramphenicol or rifampicin, the distribution of T in these fractions did not change much. Our results on T incorporation into non-growing cells indicate that the major T incorporation into bacterial cell materials is independent of biosynthetic reactions using labeled precursors produced by the assimilation of T into metabolites, but presumably depends on energy-linked conformational changes of macromolecules."}
{"id": "PMID:1086306", "title": "[Ruptured aneurysms of the sub-renal abdominal aorta].", "content": "The rupture of an aneurysm of the sub-renal aorta may give rise to several clinical presentations which it is essential to recognise in order to carry out emergency operation, e.g. attack of pain, retroperitoneal hematoma, hemoperitoneum, rupture into a hollow viscus, infective aneurysm. The special characteristics of the treatment concern resuscitation, site and type of aortic clamping, aorto-caval or aorto-digestive rupture or an infective aneurysm raising special problems. It is the vascular collapse which makes the rupture serious, an aneurysm operated as an emergency without collapse, has a mortality which differs little from a non-ruptured aneurysm, e.g. 3 deaths out of 55 operated cases. On the other hand, out of 44 aneurysms operated in acute collapse, there were 31 deaths. The complications observed are linked to the latter, e.g. cerebro-vascular accidents, acute coronary ischemia, acute ishemia of the limbs, which may also be due to embolism during operation, renal complications due to renal shock. The prevention of these complications has permitted us to reduce mortality by 40 p. 100 the last 5 years.", "contents": "[Ruptured aneurysms of the sub-renal abdominal aorta]. The rupture of an aneurysm of the sub-renal aorta may give rise to several clinical presentations which it is essential to recognise in order to carry out emergency operation, e.g. attack of pain, retroperitoneal hematoma, hemoperitoneum, rupture into a hollow viscus, infective aneurysm. The special characteristics of the treatment concern resuscitation, site and type of aortic clamping, aorto-caval or aorto-digestive rupture or an infective aneurysm raising special problems. It is the vascular collapse which makes the rupture serious, an aneurysm operated as an emergency without collapse, has a mortality which differs little from a non-ruptured aneurysm, e.g. 3 deaths out of 55 operated cases. On the other hand, out of 44 aneurysms operated in acute collapse, there were 31 deaths. The complications observed are linked to the latter, e.g. cerebro-vascular accidents, acute coronary ischemia, acute ishemia of the limbs, which may also be due to embolism during operation, renal complications due to renal shock. The prevention of these complications has permitted us to reduce mortality by 40 p. 100 the last 5 years."}
{"id": "PMID:1086307", "title": "Meningitis due to Haemophilus influenzae type e.", "content": "Haemophilus influenzae type e was isolated from the cerebrospinal fluid and blood of an 8-month-old child with meningitis.", "contents": "Meningitis due to Haemophilus influenzae type e. Haemophilus influenzae type e was isolated from the cerebrospinal fluid and blood of an 8-month-old child with meningitis."}
{"id": "PMID:1086308", "title": "Factors influencning mitotic yields from suspension cultures of normal and acute leukaemic bone marrows.", "content": "Cytogenetic studies of bone marrow are often hampered by a paucity of dividing cells. In an attempt to improve mitotic yields we have tried to promote mitotic activity in suspension cultures of bone marrow from both normal and leukaemic subjects.  Enrichment of the media always increases yield and the addition of bone marrow stimulating factor (BMSF) may do so. The best prospect for improving the yield of mitoses in acute myeloid leukaemia is to culture the marrow in an enriched medium with BMSF.", "contents": "Factors influencning mitotic yields from suspension cultures of normal and acute leukaemic bone marrows. Cytogenetic studies of bone marrow are often hampered by a paucity of dividing cells. In an attempt to improve mitotic yields we have tried to promote mitotic activity in suspension cultures of bone marrow from both normal and leukaemic subjects.  Enrichment of the media always increases yield and the addition of bone marrow stimulating factor (BMSF) may do so. The best prospect for improving the yield of mitoses in acute myeloid leukaemia is to culture the marrow in an enriched medium with BMSF."}
{"id": "PMID:1086310", "title": "Rosette inhibition test in chronic liver disease.", "content": "A role has recently been assigned to cell-mediated immunity in chronic liver diseases in addition to the well-known alterations of humoral immunity. We now report the results of the rosette inhibition test for the evaluation of T-lymphocyte \"sensitization\" in patients with different chronic liver disorders. A cell-mediated immune reaction was found in 81% of patients with chronic active hepatitis and in 71% with primary biliary cirrhosis, whereas patients with chronic persistent hepatitis showed no reaction. The correlation with the incidence of hepatitis B antigen showed that T-lymphocyte sensitization was more frequent in the group of HB-positive patients. Finally, improvement of the test was observed in four out of nine patients given immunosuppressive treatment.", "contents": "Rosette inhibition test in chronic liver disease. A role has recently been assigned to cell-mediated immunity in chronic liver diseases in addition to the well-known alterations of humoral immunity. We now report the results of the rosette inhibition test for the evaluation of T-lymphocyte \"sensitization\" in patients with different chronic liver disorders. A cell-mediated immune reaction was found in 81% of patients with chronic active hepatitis and in 71% with primary biliary cirrhosis, whereas patients with chronic persistent hepatitis showed no reaction. The correlation with the incidence of hepatitis B antigen showed that T-lymphocyte sensitization was more frequent in the group of HB-positive patients. Finally, improvement of the test was observed in four out of nine patients given immunosuppressive treatment."}
{"id": "PMID:1086311", "title": "Gastrointestinal microbleeding in normal subjects receiving acetylsalicylic acid, placebo, and R-803, a new antiinflammatory agent, in a design balanced for residual effects.", "content": "This study was undertaken to compare the relative gastrointestinal toxicity of equipotent doses of acetylsalicylic acid (ASA), 900 MG q.i.d., and a new anti-inflammatory agent, R-803, 300 mg q.i.d., against placebo. Gastrointestinal micro-bleeding was quantitated with the 61Cr-labeled erythrocyte assay. The experimental design was balanced for residual effects in the first week following any treatment. An interesting relationship between stool weight and blood loss was found to influence the microbleeding independently of the treatments themselves. All observed blood loss values were corrected by regression to a reference stool weight of 100 Gm. Final analysis of corrected values was done on arithmetic and logarithmic scales. On both scales, R-803 induced much less blood loss than ASA. A difference of 1.3 ml/day between R-803 and placebo was not statistically significant on the arithmetic scale. On the log scale, a statistically significant difference was found; but since it corresponds to 0.4 ml/day, it was not considered to be clinically significant at this dosage.", "contents": "Gastrointestinal microbleeding in normal subjects receiving acetylsalicylic acid, placebo, and R-803, a new antiinflammatory agent, in a design balanced for residual effects. This study was undertaken to compare the relative gastrointestinal toxicity of equipotent doses of acetylsalicylic acid (ASA), 900 MG q.i.d., and a new anti-inflammatory agent, R-803, 300 mg q.i.d., against placebo. Gastrointestinal micro-bleeding was quantitated with the 61Cr-labeled erythrocyte assay. The experimental design was balanced for residual effects in the first week following any treatment. An interesting relationship between stool weight and blood loss was found to influence the microbleeding independently of the treatments themselves. All observed blood loss values were corrected by regression to a reference stool weight of 100 Gm. Final analysis of corrected values was done on arithmetic and logarithmic scales. On both scales, R-803 induced much less blood loss than ASA. A difference of 1.3 ml/day between R-803 and placebo was not statistically significant on the arithmetic scale. On the log scale, a statistically significant difference was found; but since it corresponds to 0.4 ml/day, it was not considered to be clinically significant at this dosage."}
{"id": "PMID:1086312", "title": "Axonal guidance of developing optic nerves in the frog. I. Anatomy of the projection from transplanted eye primordia.", "content": "Right or left eye primordia of Shumway stage 16-18 Rana pipiens embryos were transplanted to the position normally occupied by the ear, where they developed into externally normal eyes. Ganglion cell density in the retina of the transplanted eye was significantly lower than the ganglion cell density in the animal's normal eyes. No significant difference was observed between normal and transplanted inner and outer nuclear layers. In several preparations a large piece of embryonic forebrain was included in the initial eye graft. Optic nerves from the transplanted eyes penetrated this transplanted forebrain and established a dorsocaudal growth trajectory relative to the ectopic tissue's original anterior-posterior and dorsal-ventral axes. After traversing the forebrain, the transplanted optic nerve fibers penetrated the medulla where they invaraibly grew dorsocaudally to take up and maintain a dorsolateral position in the white matter of the spinal cord. The consistent dorsocaudal trajectory established by the translocated optic tract in the ectopic forebrain and in the hindbrain and spinal cord is very similar to the growth trajectory in the normal diencephalon, suggesting that dorsocaudal growth relative to the three major axes of the neural tube is an inherent property of optic nerve fibers. These results also suggest that the polarity cues which guide normal optic tract growth are not unique to the diencephalic regions of the neural tube.", "contents": "Axonal guidance of developing optic nerves in the frog. I. Anatomy of the projection from transplanted eye primordia. Right or left eye primordia of Shumway stage 16-18 Rana pipiens embryos were transplanted to the position normally occupied by the ear, where they developed into externally normal eyes. Ganglion cell density in the retina of the transplanted eye was significantly lower than the ganglion cell density in the animal's normal eyes. No significant difference was observed between normal and transplanted inner and outer nuclear layers. In several preparations a large piece of embryonic forebrain was included in the initial eye graft. Optic nerves from the transplanted eyes penetrated this transplanted forebrain and established a dorsocaudal growth trajectory relative to the ectopic tissue's original anterior-posterior and dorsal-ventral axes. After traversing the forebrain, the transplanted optic nerve fibers penetrated the medulla where they invaraibly grew dorsocaudally to take up and maintain a dorsolateral position in the white matter of the spinal cord. The consistent dorsocaudal trajectory established by the translocated optic tract in the ectopic forebrain and in the hindbrain and spinal cord is very similar to the growth trajectory in the normal diencephalon, suggesting that dorsocaudal growth relative to the three major axes of the neural tube is an inherent property of optic nerve fibers. These results also suggest that the polarity cues which guide normal optic tract growth are not unique to the diencephalic regions of the neural tube."}
{"id": "PMID:1086313", "title": "Axonal guidance of developing optic nerves in the frog. II. electrophysiological studies of the projection from transplanted eye primordia.", "content": "When a primordial eye was transplanted to the ear position in Rana pipiens embryos, the optic nerve from the ectopic eye penetrated the medulla and invariably established a tract in the dorsolateral white matter of the ipsilateral spinal cord. In response to visual stimulation of the transplanted eye, extracellular recordings with metal microelectrodes were conducted with the spinal cords of post-metamorphic animals. Visual activity in the spinal cord could only be recorded in those experimental animals in which the transplanted optic nerve succeeded in penetrating the medulla. This activity was frequently encountered in the gray matter of the cord well below the dorsolateral position of the transplanted optic tract. The discharge characteristics and adaptation properties of the visual activity were often similar to that of optic nerve fibers from normal eyes suggesting that axons or their collaterals branch off from the transplanted optic tract and arborize within the spinal cord. However, occassionally stimulation of the transplanted eye evoked activity with adaptation and/or response characteristics unlike that of normal optic nerve fibers. Visual activity in the spincal cords of our experimental animals could be driven by moving small dark objects within circumscribed regions in the visual field of the transplanted eye. However we were unable to find any evidene of a systematic mapping of the transplanted retina within these abnormally penetrated spinal cords.", "contents": "Axonal guidance of developing optic nerves in the frog. II. electrophysiological studies of the projection from transplanted eye primordia. When a primordial eye was transplanted to the ear position in Rana pipiens embryos, the optic nerve from the ectopic eye penetrated the medulla and invariably established a tract in the dorsolateral white matter of the ipsilateral spinal cord. In response to visual stimulation of the transplanted eye, extracellular recordings with metal microelectrodes were conducted with the spinal cords of post-metamorphic animals. Visual activity in the spinal cord could only be recorded in those experimental animals in which the transplanted optic nerve succeeded in penetrating the medulla. This activity was frequently encountered in the gray matter of the cord well below the dorsolateral position of the transplanted optic tract. The discharge characteristics and adaptation properties of the visual activity were often similar to that of optic nerve fibers from normal eyes suggesting that axons or their collaterals branch off from the transplanted optic tract and arborize within the spinal cord. However, occassionally stimulation of the transplanted eye evoked activity with adaptation and/or response characteristics unlike that of normal optic nerve fibers. Visual activity in the spincal cords of our experimental animals could be driven by moving small dark objects within circumscribed regions in the visual field of the transplanted eye. However we were unable to find any evidene of a systematic mapping of the transplanted retina within these abnormally penetrated spinal cords."}
{"id": "PMID:1086314", "title": "Deficient passive and one-way active avoidance acquisition following medial forebrain bundle lesions in rats.", "content": "Cathodal electrolytic lesions of the medial forebrain bundle (MFB) at posterior hypothalamic levels in male hooded rats produced a mild, transient hypodipsia and lowered jump thresholds to footshock. The lesions produced marked deficits in passive avoidance performance in a paradigm that paired discrete, linearly incrementing footshock intensities with contact of a water spout following 48 hr of water deprivation. Intraperitoneal injections of DL-5-hydroxytryptophan (75 mg/kg), the immediate metabolic precursor of serotonin, had no effect on the passive avoidance performance of either experimental or operated control subjects. Lesions of the MFB also resulted in deficient acquisition in a one-trial step-through passive avoidance paradigm not using motivation to drink and caused a severe acquisition deficit in a one-way active avoidance task. Lesions of the septal nuclei produced lowered jump thresholds but did not affect acquisition in the first passive avoidance task. The results are interpreted as indicating a lesion-induced deficiency in fear learning, independent of the serotonergic functions of the MFB.", "contents": "Deficient passive and one-way active avoidance acquisition following medial forebrain bundle lesions in rats. Cathodal electrolytic lesions of the medial forebrain bundle (MFB) at posterior hypothalamic levels in male hooded rats produced a mild, transient hypodipsia and lowered jump thresholds to footshock. The lesions produced marked deficits in passive avoidance performance in a paradigm that paired discrete, linearly incrementing footshock intensities with contact of a water spout following 48 hr of water deprivation. Intraperitoneal injections of DL-5-hydroxytryptophan (75 mg/kg), the immediate metabolic precursor of serotonin, had no effect on the passive avoidance performance of either experimental or operated control subjects. Lesions of the MFB also resulted in deficient acquisition in a one-trial step-through passive avoidance paradigm not using motivation to drink and caused a severe acquisition deficit in a one-way active avoidance task. Lesions of the septal nuclei produced lowered jump thresholds but did not affect acquisition in the first passive avoidance task. The results are interpreted as indicating a lesion-induced deficiency in fear learning, independent of the serotonergic functions of the MFB."}
{"id": "PMID:1086316", "title": "Characterization of T and B antigen-binding cells for beta-galactosidase. III. Independence of antigen-binding cells in normal animals from antigenic stimulation.", "content": "Antigen-binding cells to beta-galactosidase were enumerated in thymus and spleen of mice of different ages and found to remain at a constant frequency throughout life. Thymic beta-galactosidase-binding cells (ZBCs),6 representing T-binding cells, showed no appreciable fluctuation, whereas splenic ZBCs, a mixture of T- and B-binding cells, were slightly depressed at birth but found at normal frequencies by 1 week of age. In addition, germfree mice, both within the 1st week after birth and after maturity, had nearly as many binding cells as did conventionally reared age-matched mice. These results considered together suggest that the ability of cells of both T and B origin to  recognize antigen, as revealed by their ability to bind beta-galactosidase, arise independently of specific or nonspecific antigenic stimulation, as part of the normal ontogenic sequence of steps of differentiation. This is consistent with the theory of clonal precommitment of T and B cells.", "contents": "Characterization of T and B antigen-binding cells for beta-galactosidase. III. Independence of antigen-binding cells in normal animals from antigenic stimulation. Antigen-binding cells to beta-galactosidase were enumerated in thymus and spleen of mice of different ages and found to remain at a constant frequency throughout life. Thymic beta-galactosidase-binding cells (ZBCs),6 representing T-binding cells, showed no appreciable fluctuation, whereas splenic ZBCs, a mixture of T- and B-binding cells, were slightly depressed at birth but found at normal frequencies by 1 week of age. In addition, germfree mice, both within the 1st week after birth and after maturity, had nearly as many binding cells as did conventionally reared age-matched mice. These results considered together suggest that the ability of cells of both T and B origin to  recognize antigen, as revealed by their ability to bind beta-galactosidase, arise independently of specific or nonspecific antigenic stimulation, as part of the normal ontogenic sequence of steps of differentiation. This is consistent with the theory of clonal precommitment of T and B cells."}
{"id": "PMID:1086317", "title": "Genetic control of the immune response in rats to the known sequential polypeptide (Tyr-Glu-Ala-Gly)n. I. Antibody responses.", "content": "In inbred rats, the antibody response to the known sequential polypeptide (Tyr-Glu-Ala-Gly)n (T-G-A-Gly)n is under the control of two independently assorting loci; (co) dominant, Ag-B-linked Ir-(T-G-A-Gly) I, controlling qualitative responsivenss, and a non-Ag-B-linked modifier locus termed Ir-(T-G-A-Gly) II, controlling the level of antibody produced. The antibody response to (T-G-A-Gly)n was solely IgG and the level of antibody produced was dependent upon Ir-(T-G-A-Gly) II for phenotypic response type.", "contents": "Genetic control of the immune response in rats to the known sequential polypeptide (Tyr-Glu-Ala-Gly)n. I. Antibody responses. In inbred rats, the antibody response to the known sequential polypeptide (Tyr-Glu-Ala-Gly)n (T-G-A-Gly)n is under the control of two independently assorting loci; (co) dominant, Ag-B-linked Ir-(T-G-A-Gly) I, controlling qualitative responsivenss, and a non-Ag-B-linked modifier locus termed Ir-(T-G-A-Gly) II, controlling the level of antibody produced. The antibody response to (T-G-A-Gly)n was solely IgG and the level of antibody produced was dependent upon Ir-(T-G-A-Gly) II for phenotypic response type."}
{"id": "PMID:1086318", "title": "Differences in the age-dependent release of a low molecular weight suppressor (LMWS) and stimulators by normal and nzb/w lymphoid organs.", "content": "The age-dependent release of soluble suppressors and stimulators of DNA synthesis by cultured thymocytes and spleen cells from C57BL/6 and BALB/c mice was compared with their release by NZB/W lymphoid organs. Spleen cells from the normal strains released high levels of suppressor early in life and gradually decresing quantities with age, NZB/W spleen cells exhibited an early deficiency followed by a later excess in the production of suppressor. These differences were quantitated by dose-response studies. Thymocytes from the normal strains released stimulatory factors throughout life. In contrast, NZB/W thymocytes stopped releasing stimulatory activity and began to produce suppressor after 2 1/2 to 4 months of life. This abnormal elaboration of suppressor by thymocytes occurred 2 months before its reappearance in the autologous spleen cell supernatant. Both the early and late-appearing (less than 1000). This activity was designed as low molecular weight suppressor (LMWS). Its aberrant production by their reported functional immunologic abnormalities. The following items were discussed: the production of LMWS by adherent spleen cells, its relationship to previously described regulators, its partial purification and initial chemical characterization, and exclusion of the naturally occurring inhibitors of lymphocyte activation, cortisol, corticosterone, cold thymidine, and cyclic AMP as the active molecule.", "contents": "Differences in the age-dependent release of a low molecular weight suppressor (LMWS) and stimulators by normal and nzb/w lymphoid organs. The age-dependent release of soluble suppressors and stimulators of DNA synthesis by cultured thymocytes and spleen cells from C57BL/6 and BALB/c mice was compared with their release by NZB/W lymphoid organs. Spleen cells from the normal strains released high levels of suppressor early in life and gradually decresing quantities with age, NZB/W spleen cells exhibited an early deficiency followed by a later excess in the production of suppressor. These differences were quantitated by dose-response studies. Thymocytes from the normal strains released stimulatory factors throughout life. In contrast, NZB/W thymocytes stopped releasing stimulatory activity and began to produce suppressor after 2 1/2 to 4 months of life. This abnormal elaboration of suppressor by thymocytes occurred 2 months before its reappearance in the autologous spleen cell supernatant. Both the early and late-appearing (less than 1000). This activity was designed as low molecular weight suppressor (LMWS). Its aberrant production by their reported functional immunologic abnormalities. The following items were discussed: the production of LMWS by adherent spleen cells, its relationship to previously described regulators, its partial purification and initial chemical characterization, and exclusion of the naturally occurring inhibitors of lymphocyte activation, cortisol, corticosterone, cold thymidine, and cyclic AMP as the active molecule."}
{"id": "PMID:1086319", "title": "A galactose-inhibitable mitogen for human lymphocytes from the sponge axinella polypoides.", "content": "Of two galactose-binding hemagglutinins isolated from the sponge Axinella polypoides, axinella I was strongly mitogenic for human peripheral blood lymphocytes, and axinella II was not. Purified T cells responded strongly and B cells weakly to axinella I. Mitogenic response, as monitored by rate of 3H-thymidine incorporation on the third day of culture, was specifically inhibited by Dgalactose, Dfucose, raffinose, or 2-deoxy-D-galactose added within 5 hr of the mitogen. Mitogenic response was correlated with degree of lymphocyte agglutination. The effectiveness of a given sugar in inhibiting mitogenic response to axinella I paralleled its potency in inhibiting precipitation of lectin by blood group substances. If an inhibitory concentration of Dgalactose was add 24 to 40 hr after mitogenic activation, rate of 3H-thymadine uptake at 72 hr was two to twenty times above the rate induced in cultures to which no galactose was added. Dgalactose at a subinhibitory concentration (10mug/ml) enhanced 3H-thymidine incorportion incorporation induced by phytohemagglutinin or Con A, an effect reversible by Dgalactose. These findings suggest that axinella I has tow antagonistic effects on human lymphocytes: a) mitogenic activation and b) depressive activity resulting from depletion of essential galactose moieties.", "contents": "A galactose-inhibitable mitogen for human lymphocytes from the sponge axinella polypoides. Of two galactose-binding hemagglutinins isolated from the sponge Axinella polypoides, axinella I was strongly mitogenic for human peripheral blood lymphocytes, and axinella II was not. Purified T cells responded strongly and B cells weakly to axinella I. Mitogenic response, as monitored by rate of 3H-thymidine incorporation on the third day of culture, was specifically inhibited by Dgalactose, Dfucose, raffinose, or 2-deoxy-D-galactose added within 5 hr of the mitogen. Mitogenic response was correlated with degree of lymphocyte agglutination. The effectiveness of a given sugar in inhibiting mitogenic response to axinella I paralleled its potency in inhibiting precipitation of lectin by blood group substances. If an inhibitory concentration of Dgalactose was add 24 to 40 hr after mitogenic activation, rate of 3H-thymadine uptake at 72 hr was two to twenty times above the rate induced in cultures to which no galactose was added. Dgalactose at a subinhibitory concentration (10mug/ml) enhanced 3H-thymidine incorportion incorporation induced by phytohemagglutinin or Con A, an effect reversible by Dgalactose. These findings suggest that axinella I has tow antagonistic effects on human lymphocytes: a) mitogenic activation and b) depressive activity resulting from depletion of essential galactose moieties."}
{"id": "PMID:1086320", "title": "Functional heterogeneity among the T-derived lymphocytes of the mouse. V. Response kinetics of peripheral T cell subpopulations.", "content": "The kinetics of the proliferative response and the appearance of effectors of helper activity after stimulation by antigen were examined in T cell subpopulations. As defined in previous papers of this series, one population, T1, is short-lived after adult thymectomy (ATx), and relatively resistant to elimination by anti-thymocyte serum (ATS). Another population, T2, is long-lived after ATx, but highly sensitive to elimination by small doses of ATS. From precursors within the T2 population, effectors of specific helper activity, after priming with antigen, appeared within 1 to 2 days and reached a maximum on day 4. The responding cells reached their peak proliferative response within 24 hr after stimulation by antigen. In contrast, helper activity arising from T1 precursors first appeared on day 3 and peaked on day 5. These cells did not reach their maximal proliferative response until 60 hr after priming. These findings indicate additional useful markers for distinguishing the T1 and T2 subpopulations and are consistent with models for T cell development in which T1 cells are virgin cells and T2 cells are memory cells.", "contents": "Functional heterogeneity among the T-derived lymphocytes of the mouse. V. Response kinetics of peripheral T cell subpopulations. The kinetics of the proliferative response and the appearance of effectors of helper activity after stimulation by antigen were examined in T cell subpopulations. As defined in previous papers of this series, one population, T1, is short-lived after adult thymectomy (ATx), and relatively resistant to elimination by anti-thymocyte serum (ATS). Another population, T2, is long-lived after ATx, but highly sensitive to elimination by small doses of ATS. From precursors within the T2 population, effectors of specific helper activity, after priming with antigen, appeared within 1 to 2 days and reached a maximum on day 4. The responding cells reached their peak proliferative response within 24 hr after stimulation by antigen. In contrast, helper activity arising from T1 precursors first appeared on day 3 and peaked on day 5. These cells did not reach their maximal proliferative response until 60 hr after priming. These findings indicate additional useful markers for distinguishing the T1 and T2 subpopulations and are consistent with models for T cell development in which T1 cells are virgin cells and T2 cells are memory cells."}
{"id": "PMID:1086321", "title": "Maturation of b lymphocytes in rates. III. Two subpopulations of memory B cells in the thoracic duct lymph differ by size, turnover rate, and surface immunoglobulin.", "content": "We examined the ability of large and small thoracic duct cells obtained from Lewis rats primed to DNP to restore the adoptive secondary anti-DNP response in irradiated syngeneic hosts given an excess of T helper cells. The large cells were four to five times more active on a per cell basis than were the small cells. However, the large cells constitute only 7 to 8% of the thoracic duct cells and, therefore, make a minor contribution to the restorative activity of the unfractionated cells. Pretreatment of thoracic duct cell donors with high specific activity 3H-TdR for 48 hr before cannulation markedly reduced the memory B cell activity of the large cells, but had little effect on that of small cells. In addition, the activity of the large cells diminished with time after primary immunization, but that of the small cells remained stable. Immunofluorescent staining of the large and small cells for surface IgM and IgG, and subsequent sorting on the fluorescence-activated cell sorter (FA CS), showed that surface IgM was present on large memory B cells, and that IgG was present on small memory B cells. The experimental results suggest that two subpopulations of memory B cells in the thoracic duct lymph differ by size, rate of turnover, persistence after primary immunization, and class of surface IgG.", "contents": "Maturation of b lymphocytes in rates. III. Two subpopulations of memory B cells in the thoracic duct lymph differ by size, turnover rate, and surface immunoglobulin. We examined the ability of large and small thoracic duct cells obtained from Lewis rats primed to DNP to restore the adoptive secondary anti-DNP response in irradiated syngeneic hosts given an excess of T helper cells. The large cells were four to five times more active on a per cell basis than were the small cells. However, the large cells constitute only 7 to 8% of the thoracic duct cells and, therefore, make a minor contribution to the restorative activity of the unfractionated cells. Pretreatment of thoracic duct cell donors with high specific activity 3H-TdR for 48 hr before cannulation markedly reduced the memory B cell activity of the large cells, but had little effect on that of small cells. In addition, the activity of the large cells diminished with time after primary immunization, but that of the small cells remained stable. Immunofluorescent staining of the large and small cells for surface IgM and IgG, and subsequent sorting on the fluorescence-activated cell sorter (FA CS), showed that surface IgM was present on large memory B cells, and that IgG was present on small memory B cells. The experimental results suggest that two subpopulations of memory B cells in the thoracic duct lymph differ by size, rate of turnover, persistence after primary immunization, and class of surface IgG."}
{"id": "PMID:1086322", "title": "Quantitative analysis of the proliferative activity induced in murine thymocytes by concanavalin A.", "content": "A quantitative analysis of the proliferative response induced in murine thymocytes by concanavalin A (Con A) is described. Exogenous 3H-thymidine labels 35 to 40% of the newly incorporated TMP residues under optimal conditions. The density label 5-bromo-2-deoxuridine (BrUdR) does not affect DNA metabolism in this system. With this nucleoside, it is shown that newly synthesized DNA is the result of semi-conservative replication, not repair. Double labeling of DNA provides a monitor for cells traversing the cell cycle (S phase to subsequent S phase). The average cycle time is 12.5 hr, and the shortest cell cycle time is 10 hr. The growing fraction of active cells is about two-thirds. The data show that different subpopulations of thymocytes begin proliferating after various times in culture. Once effectively stimulated by Con A, some of the cells can traverse the cell cycle at least twice more after the mitogen is removed.", "contents": "Quantitative analysis of the proliferative activity induced in murine thymocytes by concanavalin A. A quantitative analysis of the proliferative response induced in murine thymocytes by concanavalin A (Con A) is described. Exogenous 3H-thymidine labels 35 to 40% of the newly incorporated TMP residues under optimal conditions. The density label 5-bromo-2-deoxuridine (BrUdR) does not affect DNA metabolism in this system. With this nucleoside, it is shown that newly synthesized DNA is the result of semi-conservative replication, not repair. Double labeling of DNA provides a monitor for cells traversing the cell cycle (S phase to subsequent S phase). The average cycle time is 12.5 hr, and the shortest cell cycle time is 10 hr. The growing fraction of active cells is about two-thirds. The data show that different subpopulations of thymocytes begin proliferating after various times in culture. Once effectively stimulated by Con A, some of the cells can traverse the cell cycle at least twice more after the mitogen is removed."}
{"id": "PMID:1086323", "title": "Proliferation of murine thymic lymphocytes in vitro is mediated by the concanavalin A-induced release of a lymphokine (costimulator).", "content": "Mitogen-induced proliferation of lymphocytes may in theory result directly from the interaction of mitogen with the cells, or indirectly as a result of the mitogen-stimulated release of lymphokines. In the case of murine thymic lymphocytes exposed to concanavalin A (Con A) in tissue culture, we have determined that mitogenesis depends upon a lymphokine. Interaction of the thymic lymphocytes with lectin is necessary, but not sufficient, for mitogenesis. A lymphokine, or costimulator for mitogenesis, is released by normal spleen or thymus cells during the first 16 hr of their exposure to Con A, and in the presence of a phytomitogen it stimulates thymic mitogenesis. Under conditions of low costimulator levels, no mitogenesis follows the interaction of Con A with cells. The response of adult CBA/J mouse thymocytes to phytohemagglutinin (PHA) is very low, compared to their response to Con A. When costimulator is added to PHA, the cells respond as well as they do to Con A. Costimulator does not act through Con A-binding sites on thymus cells. Its production is dependent on both cells carrying omega surface antigen (T lymphocytes) and adherent cells of the macrophage-monocyte series. The adherent population, but not the T cells, may be heavily irradiated without affecting production of costimulator. Costimulator is not a mitogen on its own.", "contents": "Proliferation of murine thymic lymphocytes in vitro is mediated by the concanavalin A-induced release of a lymphokine (costimulator). Mitogen-induced proliferation of lymphocytes may in theory result directly from the interaction of mitogen with the cells, or indirectly as a result of the mitogen-stimulated release of lymphokines. In the case of murine thymic lymphocytes exposed to concanavalin A (Con A) in tissue culture, we have determined that mitogenesis depends upon a lymphokine. Interaction of the thymic lymphocytes with lectin is necessary, but not sufficient, for mitogenesis. A lymphokine, or costimulator for mitogenesis, is released by normal spleen or thymus cells during the first 16 hr of their exposure to Con A, and in the presence of a phytomitogen it stimulates thymic mitogenesis. Under conditions of low costimulator levels, no mitogenesis follows the interaction of Con A with cells. The response of adult CBA/J mouse thymocytes to phytohemagglutinin (PHA) is very low, compared to their response to Con A. When costimulator is added to PHA, the cells respond as well as they do to Con A. Costimulator does not act through Con A-binding sites on thymus cells. Its production is dependent on both cells carrying omega surface antigen (T lymphocytes) and adherent cells of the macrophage-monocyte series. The adherent population, but not the T cells, may be heavily irradiated without affecting production of costimulator. Costimulator is not a mitogen on its own."}
{"id": "PMID:1086324", "title": "The role of macrophages in thymocyte mitogenesis.", "content": "The thymic lymphocytes of CBA/J mice respond to the mitogen Concanavalin A (Con A) only in the presence of adherent cells of the monocyte-macrophage series. Depletion of adherent cells abrogates the response, and macrophage-rich population of cells restore it. The need for macrophages and mitogen is completely provided by irradiated splenic macrophages which have been exposed to Con A and washed free of the soluble mitogen. The mitogenmacrophage effect in this case is apparently not due to soluble factors. Even more striking than the effect of macrophages on fresh cultures of thymic lymphocytes is their ability to restimulate quiescent cells 72 hr after their first stimulation with Con A. The quiescent cells respond immediately and quantitatively to Con A in the presence of fresh macrophages. This stimulation, like that of fresh thymocytes, is also controlled by a lymphokine (\"costimulator\") produced by mixing macrophages, mitogen, ant T lymphocytes. Our data suggest a model in which two signals are required for mitogenesis. First, the interaction of macrophage, T cell, and mitogen elicits a soluble costimulator, which is itself not mitogenic. Secondly, in the presence of costimulator, the mitogen (either soluble, or, more efficiently, bound to macrophages) induces a proliferative response in the T cell.", "contents": "The role of macrophages in thymocyte mitogenesis. The thymic lymphocytes of CBA/J mice respond to the mitogen Concanavalin A (Con A) only in the presence of adherent cells of the monocyte-macrophage series. Depletion of adherent cells abrogates the response, and macrophage-rich population of cells restore it. The need for macrophages and mitogen is completely provided by irradiated splenic macrophages which have been exposed to Con A and washed free of the soluble mitogen. The mitogenmacrophage effect in this case is apparently not due to soluble factors. Even more striking than the effect of macrophages on fresh cultures of thymic lymphocytes is their ability to restimulate quiescent cells 72 hr after their first stimulation with Con A. The quiescent cells respond immediately and quantitatively to Con A in the presence of fresh macrophages. This stimulation, like that of fresh thymocytes, is also controlled by a lymphokine (\"costimulator\") produced by mixing macrophages, mitogen, ant T lymphocytes. Our data suggest a model in which two signals are required for mitogenesis. First, the interaction of macrophage, T cell, and mitogen elicits a soluble costimulator, which is itself not mitogenic. Secondly, in the presence of costimulator, the mitogen (either soluble, or, more efficiently, bound to macrophages) induces a proliferative response in the T cell."}
{"id": "PMID:1086325", "title": "Reversal of cell surface abnormalities of T lymphocytes in hodgkin's disease after in vitro incubation in fetal sera.", "content": "The capacity of periphal blood lymphocytes from patients with untreated Hodgkin's disease to form E rosettes with sheep erythrocytes and to respond in vitro to PHA stimulation were found to be profoundly impaired. In 49% of the patients, the percentage of E rosette-forming cells (E-RFC) was more than two standard deviations below the mean for normal donors. Overnight incubation of the peripheral blood lymhocytes from these patients in culture media containing 20% fetal calf serum was followed by restoration of the percentage of E-RFC up to normal levels. Similar results have been observed after incubation in fetal human serum, but not in adult human AB serum or adult bovine serum. Incubation of peripheral blood lymphocytes from untreated patients in20% fetal calf serum also resulted in a remarkable restoration of their capacity to respond normally to PHA. Possible mechanisms involved in these reversible cell surface and in vitro lymphocyte function abnormalities in Hodgkin's disease are discussed.", "contents": "Reversal of cell surface abnormalities of T lymphocytes in hodgkin's disease after in vitro incubation in fetal sera. The capacity of periphal blood lymphocytes from patients with untreated Hodgkin's disease to form E rosettes with sheep erythrocytes and to respond in vitro to PHA stimulation were found to be profoundly impaired. In 49% of the patients, the percentage of E rosette-forming cells (E-RFC) was more than two standard deviations below the mean for normal donors. Overnight incubation of the peripheral blood lymhocytes from these patients in culture media containing 20% fetal calf serum was followed by restoration of the percentage of E-RFC up to normal levels. Similar results have been observed after incubation in fetal human serum, but not in adult human AB serum or adult bovine serum. Incubation of peripheral blood lymphocytes from untreated patients in20% fetal calf serum also resulted in a remarkable restoration of their capacity to respond normally to PHA. Possible mechanisms involved in these reversible cell surface and in vitro lymphocyte function abnormalities in Hodgkin's disease are discussed."}
{"id": "PMID:1086326", "title": "An improved technique for obtaining E rosettes with human lymphocytes and its use for B cell purification.", "content": "The standard E rosette method and two previously described methods claimed to give improved E rosetting for enumeration of human T lymphocytes have been compared with respect to the speed of rosette formation, and the mechanical stability of the rosettes formed. Following rosette formation with the three methods, the lymphocyte-erythrocyte suspensions were sedimented on Ficoll-Isopaque to deplete them of rosette-forming cells and red cells. The purified lymphocyte preparations were tested for B and T cell markers to determine the degree of contamination with T cells. One of the improved rosetting methods was clearly better than the others, and led to the recovery of B lymphocytes with a contamination of only 2.0+/-1.9 per cent of T lymphocytes.", "contents": "An improved technique for obtaining E rosettes with human lymphocytes and its use for B cell purification. The standard E rosette method and two previously described methods claimed to give improved E rosetting for enumeration of human T lymphocytes have been compared with respect to the speed of rosette formation, and the mechanical stability of the rosettes formed. Following rosette formation with the three methods, the lymphocyte-erythrocyte suspensions were sedimented on Ficoll-Isopaque to deplete them of rosette-forming cells and red cells. The purified lymphocyte preparations were tested for B and T cell markers to determine the degree of contamination with T cells. One of the improved rosetting methods was clearly better than the others, and led to the recovery of B lymphocytes with a contamination of only 2.0+/-1.9 per cent of T lymphocytes."}
{"id": "PMID:1086338", "title": "Role of karyoplasm in the emergence of capacity of egg cytoplasm to induce DNA synthesis in transplanted sperm nuclei.", "content": "The behaviour of sperm nuclei was studied both in the cytoplasm of intact toad oocytes undergoing maturation and the cytoplasm of oocytes matured without germinal vesicles. The behaviour of the nuclei of pronase-treated sperm injected in the mature egg cytoplasm was shown to be exactly similar to that of the sperm nucleus after fertilization, i.e. they swelled, synthesized DNA, and divided. No changes in such sperm nuclei could be detected in the cytoplasm of the oocytes matured without germinal vesicles.", "contents": "Role of karyoplasm in the emergence of capacity of egg cytoplasm to induce DNA synthesis in transplanted sperm nuclei. The behaviour of sperm nuclei was studied both in the cytoplasm of intact toad oocytes undergoing maturation and the cytoplasm of oocytes matured without germinal vesicles. The behaviour of the nuclei of pronase-treated sperm injected in the mature egg cytoplasm was shown to be exactly similar to that of the sperm nucleus after fertilization, i.e. they swelled, synthesized DNA, and divided. No changes in such sperm nuclei could be detected in the cytoplasm of the oocytes matured without germinal vesicles."}
{"id": "PMID:1086339", "title": "Control of B-lymphocyte function. I. Inactivation of mitogenesis by interactions with surface immunoglobulin and Fc-receptor molecules.", "content": "Mouse spleen cells were incubated with anti-Ig antibodies for 1 h, washed, exposed to LPS for 1 h, washed, and their DNA synthetic responses assayed 2 days later. It was shown that the 1-h incubation with anti-Ig antibodies produced a profound, internal, and long lasting state of inactivation in the B cells. Experiments with anti-Ig fragments and other ligands showed that the inactivation occurred optimally when both surface Ig molecules and Fc receptors were bound simultaneously. The role of the classical capping and clearing cycle was also investigated. It was shown that capping and clearing were neither necessary nor sufficient for the inactivation to occur, and that the signals, but only secondarily the ligands themselves, were transmitted across the membrane. Capping and clearing were viewed as a natural regulatory mechanism by which the B cell attempts to clear its membrane of perturbations and signals from the exterior.", "contents": "Control of B-lymphocyte function. I. Inactivation of mitogenesis by interactions with surface immunoglobulin and Fc-receptor molecules. Mouse spleen cells were incubated with anti-Ig antibodies for 1 h, washed, exposed to LPS for 1 h, washed, and their DNA synthetic responses assayed 2 days later. It was shown that the 1-h incubation with anti-Ig antibodies produced a profound, internal, and long lasting state of inactivation in the B cells. Experiments with anti-Ig fragments and other ligands showed that the inactivation occurred optimally when both surface Ig molecules and Fc receptors were bound simultaneously. The role of the classical capping and clearing cycle was also investigated. It was shown that capping and clearing were neither necessary nor sufficient for the inactivation to occur, and that the signals, but only secondarily the ligands themselves, were transmitted across the membrane. Capping and clearing were viewed as a natural regulatory mechanism by which the B cell attempts to clear its membrane of perturbations and signals from the exterior."}
{"id": "PMID:1086340", "title": "Inhibition of the development of the cellular slime mould Dictyostelium discoideum by omega-aminocarboxylic acids.", "content": "Four omega-aminocarboxylic acids - epsilon-aminocaproic acid (EACA), trans-4-aminomethylcyclohexane-1-carboxylic acid (t-AMCHA), p-aminomethylbenzoic acid (PAMBA) and omega-aminocaprylic acid (OACA) -- prevented fruiting body formation of the cellular slime mould Dictyostelium discoideum. At concentrations of 40 mM, 75 mM, 10 mM and 5 mM, respectively, they allowed aggregation but prevented all further development at 24 degrees C. At lower concentrations, EACA allowed fruiting body formation but with a reduced number of spores per fruiting body. Only t-AMCHA had a significant inhibitory effect on the growth of myxamoebae. EACA affected development only if it was present between 8 and 16 h after the cells were deposited on the filters. Its effect was enhanced by high salt concentrations and by higher temperature, and was also dependent on the manner in which the cells were grown. Only strains capable of axenic growth displayed this sensitivity to EACA, although strains carrying only one of the genetic markers for axenic growth (axe A) were partially sensitive.", "contents": "Inhibition of the development of the cellular slime mould Dictyostelium discoideum by omega-aminocarboxylic acids. Four omega-aminocarboxylic acids - epsilon-aminocaproic acid (EACA), trans-4-aminomethylcyclohexane-1-carboxylic acid (t-AMCHA), p-aminomethylbenzoic acid (PAMBA) and omega-aminocaprylic acid (OACA) -- prevented fruiting body formation of the cellular slime mould Dictyostelium discoideum. At concentrations of 40 mM, 75 mM, 10 mM and 5 mM, respectively, they allowed aggregation but prevented all further development at 24 degrees C. At lower concentrations, EACA allowed fruiting body formation but with a reduced number of spores per fruiting body. Only t-AMCHA had a significant inhibitory effect on the growth of myxamoebae. EACA affected development only if it was present between 8 and 16 h after the cells were deposited on the filters. Its effect was enhanced by high salt concentrations and by higher temperature, and was also dependent on the manner in which the cells were grown. Only strains capable of axenic growth displayed this sensitivity to EACA, although strains carrying only one of the genetic markers for axenic growth (axe A) were partially sensitive."}
{"id": "PMID:1086341", "title": "The effect of epsilon-aminocaproic acid on biochemical changes in the development of the cellular slime mould Dictyostelium discoideum.", "content": "epsilon-Aminocaproic acid (EACA) inhibited the development of Dictyostelium discoideum strain AX2 after the aggregation stage. Biochemical changes that occurred early in development (loss of cellular protein, RNA and carbohydrate; increase in the specific activity of N-acetylglucosaminidase, alpha-mannosidase, threonine deaminase and leucine aminopeptidase) were not affected by concentrations of EACA which blocked development; but biochemical changes that occurred later (synthesis of carbohydrate, increase in the specific activity of UDP-glucose pyrophosphorylase)  were inhibited. Spores from fruiting bodies formed in the presence of low concentrations of EACA were larger, more spherical and less able to survive heat treatment than spores from fruiting bodies of control (no EACA) cells.", "contents": "The effect of epsilon-aminocaproic acid on biochemical changes in the development of the cellular slime mould Dictyostelium discoideum. epsilon-Aminocaproic acid (EACA) inhibited the development of Dictyostelium discoideum strain AX2 after the aggregation stage. Biochemical changes that occurred early in development (loss of cellular protein, RNA and carbohydrate; increase in the specific activity of N-acetylglucosaminidase, alpha-mannosidase, threonine deaminase and leucine aminopeptidase) were not affected by concentrations of EACA which blocked development; but biochemical changes that occurred later (synthesis of carbohydrate, increase in the specific activity of UDP-glucose pyrophosphorylase)  were inhibited. Spores from fruiting bodies formed in the presence of low concentrations of EACA were larger, more spherical and less able to survive heat treatment than spores from fruiting bodies of control (no EACA) cells."}
{"id": "PMID:1086342", "title": "Psychopathology observed on follow-up after coronary bypass surgery.", "content": "Forty-six of 51 coronary bypass patients whose inhospital postoperative adjustment was reported earlier, were studied about 18 months postoperatively. In addition, 32 of 46 cardiac valvular surgery patients were seen as a comparison group. The incidence of postoperative psychiatric symptoms observed in the hospital, eight of 51 (16 per cent), and on follow-up, seven of 46 (15 per cent), was very similar; however, the patients who had these psychiatric symptoms comprised two almost entirely different groups. There were no indications that patients who developed psychiatric symptoms in the immediate postoperative period than patients asymptomatic postoperatively. There was a significant relationship between preoperative psychiatric illness and symptoms in the follow-up period.", "contents": "Psychopathology observed on follow-up after coronary bypass surgery. Forty-six of 51 coronary bypass patients whose inhospital postoperative adjustment was reported earlier, were studied about 18 months postoperatively. In addition, 32 of 46 cardiac valvular surgery patients were seen as a comparison group. The incidence of postoperative psychiatric symptoms observed in the hospital, eight of 51 (16 per cent), and on follow-up, seven of 46 (15 per cent), was very similar; however, the patients who had these psychiatric symptoms comprised two almost entirely different groups. There were no indications that patients who developed psychiatric symptoms in the immediate postoperative period than patients asymptomatic postoperatively. There was a significant relationship between preoperative psychiatric illness and symptoms in the follow-up period."}
{"id": "PMID:1086343", "title": "The role of intraneuronal amine levels in the feedback control of dopamine, noradrenaline and 5-hydroxytryptamine synthesis in rat brain.", "content": "The influence of varying brain levels of dopamine, noradrenaline and 5-HT on their respective synthesis rates has been investigated. The first step in monoamine synthesis was studied in vivo by measuring the accumulation of dopa and 5-hydroxytryptophan after inhibition of the aromatic L-amino acid decarboxylase. Variations in monoamine levels were obtained by combined treatment with inhibitors of the decarboxylase (NSD 1015 or Ro 4-4602) and of monoamine oxidase (pargyline). An increase in monoamine levels by pargyline was found to inhibit the synthesis of dopamine, noradrenaline and 5-HT. Conversely, a decrease in monoamine levels induced by the decarboxylase inhibitor Ro 4-4602 appeared to stimulate dopamine and noradrenaline synthesis but had no effect on 5-HT synthesis. The influence of varying levels of dopamine and noradrenaline on the synthesis of these amines could still be demonstrated after blockade of dopamine receptos and of alpha-adrenergic (noradrenaline) receptors by haloperidol, suggesting that the mechanism involved in this feedback control is mediated via end-product inhibition of tyrosine hydroxylase. On the other hand, the stimulating influence of haloperidol on the synthesis of catecholamines does not seem to be directly related to changes in catecholamine levels. It is concluded that the short-term control of catecholamine synthesis presumably involves two independent feedback mechanisms, one intraneuronal mechanism operating via end-product inhibition, and one synaptic mechanism mediated via dopamine and noradrenaline receptors, respectively. Both pre- and postsynaptic receptors may be involved in the latter mechanism.", "contents": "The role of intraneuronal amine levels in the feedback control of dopamine, noradrenaline and 5-hydroxytryptamine synthesis in rat brain. The influence of varying brain levels of dopamine, noradrenaline and 5-HT on their respective synthesis rates has been investigated. The first step in monoamine synthesis was studied in vivo by measuring the accumulation of dopa and 5-hydroxytryptophan after inhibition of the aromatic L-amino acid decarboxylase. Variations in monoamine levels were obtained by combined treatment with inhibitors of the decarboxylase (NSD 1015 or Ro 4-4602) and of monoamine oxidase (pargyline). An increase in monoamine levels by pargyline was found to inhibit the synthesis of dopamine, noradrenaline and 5-HT. Conversely, a decrease in monoamine levels induced by the decarboxylase inhibitor Ro 4-4602 appeared to stimulate dopamine and noradrenaline synthesis but had no effect on 5-HT synthesis. The influence of varying levels of dopamine and noradrenaline on the synthesis of these amines could still be demonstrated after blockade of dopamine receptos and of alpha-adrenergic (noradrenaline) receptors by haloperidol, suggesting that the mechanism involved in this feedback control is mediated via end-product inhibition of tyrosine hydroxylase. On the other hand, the stimulating influence of haloperidol on the synthesis of catecholamines does not seem to be directly related to changes in catecholamine levels. It is concluded that the short-term control of catecholamine synthesis presumably involves two independent feedback mechanisms, one intraneuronal mechanism operating via end-product inhibition, and one synaptic mechanism mediated via dopamine and noradrenaline receptors, respectively. Both pre- and postsynaptic receptors may be involved in the latter mechanism."}
{"id": "PMID:1086344", "title": "[Embryonic pineal organ of Lacerta vivipara J. II. Electron microscopic study of 3H-5-hydroxytryptophan (3H-5-HTP) incorporation in the secretory rudimentary photoreceptor cells (SRP). The gradient of biochemical differentiation (author's transl)].", "content": "As the biosynthesis of indoleamines in Lacerta vivipara embryos from stage 36 to 40 is concerned, our ultrastructural radioautographic studies reveal a selective incorporation and metabolism of 3H-5-HTP in one of the two \"sensory\" cell types, the Secretory Rudimentary Photoreceptor cells (SRP). Specific radioautographic reactions are constantly for the most part located on the dense secretory granules which are stored in the basal processes of the SRP and originate in the Golgi complex. During ontogenesis, the amount of autoradiographically demonstrable tritiated indoleamines and the number of dense secretory granules increase at the same rate. The combination of electron microscopic and radioautographic studies shows that, during ontogenesis, a close relation exists between the cytological differentiation of the SRP and their capacity to synthesize and store indoleamines.", "contents": "[Embryonic pineal organ of Lacerta vivipara J. II. Electron microscopic study of 3H-5-hydroxytryptophan (3H-5-HTP) incorporation in the secretory rudimentary photoreceptor cells (SRP). The gradient of biochemical differentiation (author's transl)]. As the biosynthesis of indoleamines in Lacerta vivipara embryos from stage 36 to 40 is concerned, our ultrastructural radioautographic studies reveal a selective incorporation and metabolism of 3H-5-HTP in one of the two \"sensory\" cell types, the Secretory Rudimentary Photoreceptor cells (SRP). Specific radioautographic reactions are constantly for the most part located on the dense secretory granules which are stored in the basal processes of the SRP and originate in the Golgi complex. During ontogenesis, the amount of autoradiographically demonstrable tritiated indoleamines and the number of dense secretory granules increase at the same rate. The combination of electron microscopic and radioautographic studies shows that, during ontogenesis, a close relation exists between the cytological differentiation of the SRP and their capacity to synthesize and store indoleamines."}
{"id": "PMID:1086345", "title": "Measurements of stiffness of extraocular muscles of the rabbit.", "content": "1. The stiffness of the eye and the extraocular muscles of the conscious rabbit was measured and compared with similar measurements made on human subjects. 2. The extraocular muscles of the rabbit developed less than 0.5 g at optimal isometric length when the contralateral eye was in \"primary position\". Deviations of the eye of the rabbit from primary position were accomplished by an increase in force of the agonist muscle with only a nominal decrease in force in the antagonist. 3. With all muscles attached to the globe, the stiffness of the eye for displacements of up to 35 degrees from primary position was 0.11 +/- 0.03 g/deg. 4. The stiffness of the human eye to lateral rotations was 1.09 +/- 0.24 g/deg. Hence, identical disturbances in force would cause deviations in the position of the eye of the rabbit which are nearly an order of magnitude greater than those of man. 5. The stiffness of the extraocular muscles of the rabbit was sensitive to levels of muscle activation produced either by indirect electrical stimulation or by the vestibuloocular reflex. 6. The implications of these findings for the development of eye position control in animals with good binocular vision are discussed.", "contents": "Measurements of stiffness of extraocular muscles of the rabbit. 1. The stiffness of the eye and the extraocular muscles of the conscious rabbit was measured and compared with similar measurements made on human subjects. 2. The extraocular muscles of the rabbit developed less than 0.5 g at optimal isometric length when the contralateral eye was in \"primary position\". Deviations of the eye of the rabbit from primary position were accomplished by an increase in force of the agonist muscle with only a nominal decrease in force in the antagonist. 3. With all muscles attached to the globe, the stiffness of the eye for displacements of up to 35 degrees from primary position was 0.11 +/- 0.03 g/deg. 4. The stiffness of the human eye to lateral rotations was 1.09 +/- 0.24 g/deg. Hence, identical disturbances in force would cause deviations in the position of the eye of the rabbit which are nearly an order of magnitude greater than those of man. 5. The stiffness of the extraocular muscles of the rabbit was sensitive to levels of muscle activation produced either by indirect electrical stimulation or by the vestibuloocular reflex. 6. The implications of these findings for the development of eye position control in animals with good binocular vision are discussed."}
{"id": "PMID:1086346", "title": "Correlation of light-induced changes in retinal extracellular potassium concentration with c-wave of the electroretinogram.", "content": "1. Double-barrel, potassium-specific microelectrodes have been used to measure light-induced transient changes in [K+]o in the frog eye cup preparation. These changes in [K+]o have been termed the potassioretinogram (KRG). 2. The KRG consists of two components: a rapid increase in [K+]o in the proximal retina and a slow decrease in [K+]o in the distal retina. 3. The KRG decrease has the rhodopsin action spectrum, is maximal in the photoreceptor layer, persists after aspartate treatment, and has an increment threshold curve which saturates at moderate background intensities. The rhodopsin rods are, therefore, most likely the only neurons which generate this ionic change, although the M\u00fcller (glial) cells may also be involved in this process. 4. The KRG decrease has the same time course as the c-wave of the electroretinogram for all variations in the stimulus parameters, including intensity, duration, and chromaticity. 5. It is suggested that the c-wave may be produced by the pigment epithelial cells as they hyperpolarize in response to the decrease in [K+]o around the photoreceptors.", "contents": "Correlation of light-induced changes in retinal extracellular potassium concentration with c-wave of the electroretinogram. 1. Double-barrel, potassium-specific microelectrodes have been used to measure light-induced transient changes in [K+]o in the frog eye cup preparation. These changes in [K+]o have been termed the potassioretinogram (KRG). 2. The KRG consists of two components: a rapid increase in [K+]o in the proximal retina and a slow decrease in [K+]o in the distal retina. 3. The KRG decrease has the rhodopsin action spectrum, is maximal in the photoreceptor layer, persists after aspartate treatment, and has an increment threshold curve which saturates at moderate background intensities. The rhodopsin rods are, therefore, most likely the only neurons which generate this ionic change, although the M\u00fcller (glial) cells may also be involved in this process. 4. The KRG decrease has the same time course as the c-wave of the electroretinogram for all variations in the stimulus parameters, including intensity, duration, and chromaticity. 5. It is suggested that the c-wave may be produced by the pigment epithelial cells as they hyperpolarize in response to the decrease in [K+]o around the photoreceptors."}
{"id": "PMID:1086347", "title": "Adaptive gain control of vestibuloocular reflex by the cerebellum.", "content": "1. The gain of the vestibuloocular reflex (slow-phase eye velocity/head velocity) was measured in 17 adult cats. 2. The gain of the reflex, in the dark, was 0.90 (+/-0.15 SD) over the frequency range 0.03-1.2 Hz. 3. In the range 0.01-0.15 Hz, the phase behaved as though the overall reflex time constant were 12 s or greater. The cupula time constant is 4 s. Therefore, the central part of the reflex must manipulate the canal signal to improve its low-frequency response by a factor of at least three. 4. When the cats wore left-right reversing prisms chronically and were also rotated for 2 h every day, the reflex underwent large, plastic changes. The gain, tested in the dark, decreased by 93% at 0.05 Hz and 55% at 1.2 Hz. In effect, the low-frequency response was abolished. The process took about 8 days. 5. In the light, with reversed vision, the gain decreased further and, at low frequencies, the eye movements did reverse in direction. 6. When the vestibulocerebellum was removed, the gain, in the dark, rose to about 1.17 and the plastic changes caused by reversing prisms were completely abolished. 7. Reversing prisms create vestibuloocular dysmetria. The change in gain they produce is considered to be an adaptive response designed to reduce image motion on the retina during head movements. The vestibulocerebellum is necessary for this adaptive process. It is proposed that detecting and repairing dysmetria (of natural origin) is an important cerebellar function.", "contents": "Adaptive gain control of vestibuloocular reflex by the cerebellum. 1. The gain of the vestibuloocular reflex (slow-phase eye velocity/head velocity) was measured in 17 adult cats. 2. The gain of the reflex, in the dark, was 0.90 (+/-0.15 SD) over the frequency range 0.03-1.2 Hz. 3. In the range 0.01-0.15 Hz, the phase behaved as though the overall reflex time constant were 12 s or greater. The cupula time constant is 4 s. Therefore, the central part of the reflex must manipulate the canal signal to improve its low-frequency response by a factor of at least three. 4. When the cats wore left-right reversing prisms chronically and were also rotated for 2 h every day, the reflex underwent large, plastic changes. The gain, tested in the dark, decreased by 93% at 0.05 Hz and 55% at 1.2 Hz. In effect, the low-frequency response was abolished. The process took about 8 days. 5. In the light, with reversed vision, the gain decreased further and, at low frequencies, the eye movements did reverse in direction. 6. When the vestibulocerebellum was removed, the gain, in the dark, rose to about 1.17 and the plastic changes caused by reversing prisms were completely abolished. 7. Reversing prisms create vestibuloocular dysmetria. The change in gain they produce is considered to be an adaptive response designed to reduce image motion on the retina during head movements. The vestibulocerebellum is necessary for this adaptive process. It is proposed that detecting and repairing dysmetria (of natural origin) is an important cerebellar function."}
{"id": "PMID:1086348", "title": "Peripheral nerve stimulation in the treatment of intractable pain.", "content": "Peripheral nerve stimulating devices were implanted for pain control in 33 patients with a variety of disabling chronic pain conditions, which had persisted despite usual medical and surgical therapy. The implants were placed on major nerves innervating the area of the patient's pain. Records were obtained of each patient's stated relief from pain produced by nerve stimulation, along with assessments of narcotic withdrawal, ability to return to work, sleep pattern, and relief from depression. Based on these five criteria 17 patients were judged to be treatment failures, while eight patients had excellent results, and seven had intermediate results. Twelve of the failures were in patients with either low back pain with sciatica, or pain from metastatic disease. The most dramatic successes occurred in patients with peripheral nerve trauma. The incidence of complications has been low, and two patients have used the stimulator for 5 years without adverse effects. Techniques of peripheral stimulator implantation, possible mechanisms of action, and conclusions regarding peripheral nerve stimulation in the treatment of chronic pain are discussed.", "contents": "Peripheral nerve stimulation in the treatment of intractable pain. Peripheral nerve stimulating devices were implanted for pain control in 33 patients with a variety of disabling chronic pain conditions, which had persisted despite usual medical and surgical therapy. The implants were placed on major nerves innervating the area of the patient's pain. Records were obtained of each patient's stated relief from pain produced by nerve stimulation, along with assessments of narcotic withdrawal, ability to return to work, sleep pattern, and relief from depression. Based on these five criteria 17 patients were judged to be treatment failures, while eight patients had excellent results, and seven had intermediate results. Twelve of the failures were in patients with either low back pain with sciatica, or pain from metastatic disease. The most dramatic successes occurred in patients with peripheral nerve trauma. The incidence of complications has been low, and two patients have used the stimulator for 5 years without adverse effects. Techniques of peripheral stimulator implantation, possible mechanisms of action, and conclusions regarding peripheral nerve stimulation in the treatment of chronic pain are discussed."}
{"id": "PMID:1086351", "title": "Incidence of ampicillin-resistant Hemophilus influenzae in otitis media.", "content": "Ampicillin resistance in Hemophilus influenzae type b causing serious infections is appreciated, but little is known about ampicillin resistance in nonencapsulated strains causing otitis media. The ampicillin sensitivity of 984 middle-ear isolates of H. influenzae from children in Huntsville, Ala. obtained from 1970-1976, was examined: Nine AmpR isolates were found: one in 1973, two in 1974, three in 1975, and three in the first five months of 1976. Seven strains were nonencapsulated; two were type b. All nine produced beta-lactomase. The incidence of ampicillin resistance in strains causing otitis media increased from 0.6% in 1973 to 2.4% in 1976. AmpR H. influenzae infection should be suspected in situations where ampicillin therapy of otitis media is unsuccessful.", "contents": "Incidence of ampicillin-resistant Hemophilus influenzae in otitis media. Ampicillin resistance in Hemophilus influenzae type b causing serious infections is appreciated, but little is known about ampicillin resistance in nonencapsulated strains causing otitis media. The ampicillin sensitivity of 984 middle-ear isolates of H. influenzae from children in Huntsville, Ala. obtained from 1970-1976, was examined: Nine AmpR isolates were found: one in 1973, two in 1974, three in 1975, and three in the first five months of 1976. Seven strains were nonencapsulated; two were type b. All nine produced beta-lactomase. The incidence of ampicillin resistance in strains causing otitis media increased from 0.6% in 1973 to 2.4% in 1976. AmpR H. influenzae infection should be suspected in situations where ampicillin therapy of otitis media is unsuccessful."}
{"id": "PMID:1086355", "title": "Identification of the pace-maker current in frog atrium.", "content": "1. The nature and interactions of the membrane currents underlying induced pace-maker activity in frog atrial muscle have been investigated using a double sucrose gap technique. 2. The membrane current which controls the speed of the atrial pacemaker depolarization (the pace-maker current, ip), is shown to be an outward current activated within the plateau potential range of a normal action potential. The subsequent deactivation of ip at more negative potentials unmasks the depolarizing action of time-independent inward membrane currents so that a pace-maker potential can result. 3. The deactivation of ip over a limited potential range (between about -30 and -60 mV) can be reliably recorded by switching on the voltage clamp during an induced pace-maker depolarization. 4. Investigation of the time and voltage-dependent behaviour of ip over a much wider potential range is less straightforward. How ip can be separated from other components of outward current present in the decay tails following square voltage clamp depolarizations is described. 5. The majority of such current decay tails contain three components of outward current. It appears that two of these components, one of which is ip, are true Hodgkin-Huxley conductance systems chiefly carrying potassium ions. 6. The nature of the third current, which decays very slowly at moderate membrane potentials (about -40 mV), is discussed and reasons are briefly given for considering it to result from the accumulation of potassium ions in extracellular spaces. Preliminary evidence that potassium depletion occurs at potentials negative to the resting potential of the trabeculum is also presented. 7. Because of the obvious complexities involved, a quantitative analysis of the atrial outward currents is not attempted here but forms the subject of a following paper (Brown, Clark & Noble, 1976a).", "contents": "Identification of the pace-maker current in frog atrium. 1. The nature and interactions of the membrane currents underlying induced pace-maker activity in frog atrial muscle have been investigated using a double sucrose gap technique. 2. The membrane current which controls the speed of the atrial pacemaker depolarization (the pace-maker current, ip), is shown to be an outward current activated within the plateau potential range of a normal action potential. The subsequent deactivation of ip at more negative potentials unmasks the depolarizing action of time-independent inward membrane currents so that a pace-maker potential can result. 3. The deactivation of ip over a limited potential range (between about -30 and -60 mV) can be reliably recorded by switching on the voltage clamp during an induced pace-maker depolarization. 4. Investigation of the time and voltage-dependent behaviour of ip over a much wider potential range is less straightforward. How ip can be separated from other components of outward current present in the decay tails following square voltage clamp depolarizations is described. 5. The majority of such current decay tails contain three components of outward current. It appears that two of these components, one of which is ip, are true Hodgkin-Huxley conductance systems chiefly carrying potassium ions. 6. The nature of the third current, which decays very slowly at moderate membrane potentials (about -40 mV), is discussed and reasons are briefly given for considering it to result from the accumulation of potassium ions in extracellular spaces. Preliminary evidence that potassium depletion occurs at potentials negative to the resting potential of the trabeculum is also presented. 7. Because of the obvious complexities involved, a quantitative analysis of the atrial outward currents is not attempted here but forms the subject of a following paper (Brown, Clark & Noble, 1976a)."}
{"id": "PMID:1086356", "title": "Analysis of pace-maker and repolarization currents in frog atrial muscle.", "content": "1. A quantitative analysis of the time-dependent component of outward membrane current in atrial wall trabeculae from Rana catesbeiana and Rana ridibunda has been carried out using a double sucrose gap technique. 2. Separation of the different components of delayed outward current was hampered by the sigmoid onset of one of the outward current systems ixfast and by the development of potassium ion accumulation which prevented current activation from reaching a steady state at positive membrane potentials. Semilogarithmic analysis of positive current decay tails recorded immediately following square voltage clamp depolarizations was therefore used to separate the two membrane conductance components ixfast and ixslow and the third component, attributable to potassium ion accumulation, which was almost invariably present in the tails. 3. It is shown that inaccuracies in this method of semilogarithmic separation of components caused by visual assessment of the i3 (accumulation) line are minor compared with the large changes in the time constants of ixfast and more especially of ixslow which would result from ignoring the potassium accumulation component. 4. Such semilogarithmic separation of the three components of outward current gave separate activation curves for each of the two membrane conductance components, ixfast and ixslow. 5. Measurement of 'total' activation curves in which all components of outward current were represented could be made more easily and fairly reliably. The position and shape of these activation curves on the voltage axis were found to closely resemble those obtained by three component separation. It is therefore suggested that such a simplified analysis reflects the properties of the individual currents sufficiently well for it to be of use in preliminary studies of, for example, drug action. 6. The kinetic properties of the atrial outward currents have been investigated over a wide potential range. Because of the presence of potassium ion accumulation, an indirect method of obtaining the average value of 1/gamma for outward current decay at negative potentials had to be employed. 7. It is shown that some degree of inward-going rectification is associated with the outward current systems of frog atrium. 8. The possible reasons for the differences between the analysis presented here and those presented earlier by us (Brown & Noble, 1969a, b) and by Ojeda & Rougier (1974) are discussed.", "contents": "Analysis of pace-maker and repolarization currents in frog atrial muscle. 1. A quantitative analysis of the time-dependent component of outward membrane current in atrial wall trabeculae from Rana catesbeiana and Rana ridibunda has been carried out using a double sucrose gap technique. 2. Separation of the different components of delayed outward current was hampered by the sigmoid onset of one of the outward current systems ixfast and by the development of potassium ion accumulation which prevented current activation from reaching a steady state at positive membrane potentials. Semilogarithmic analysis of positive current decay tails recorded immediately following square voltage clamp depolarizations was therefore used to separate the two membrane conductance components ixfast and ixslow and the third component, attributable to potassium ion accumulation, which was almost invariably present in the tails. 3. It is shown that inaccuracies in this method of semilogarithmic separation of components caused by visual assessment of the i3 (accumulation) line are minor compared with the large changes in the time constants of ixfast and more especially of ixslow which would result from ignoring the potassium accumulation component. 4. Such semilogarithmic separation of the three components of outward current gave separate activation curves for each of the two membrane conductance components, ixfast and ixslow. 5. Measurement of 'total' activation curves in which all components of outward current were represented could be made more easily and fairly reliably. The position and shape of these activation curves on the voltage axis were found to closely resemble those obtained by three component separation. It is therefore suggested that such a simplified analysis reflects the properties of the individual currents sufficiently well for it to be of use in preliminary studies of, for example, drug action. 6. The kinetic properties of the atrial outward currents have been investigated over a wide potential range. Because of the presence of potassium ion accumulation, an indirect method of obtaining the average value of 1/gamma for outward current decay at negative potentials had to be employed. 7. It is shown that some degree of inward-going rectification is associated with the outward current systems of frog atrium. 8. The possible reasons for the differences between the analysis presented here and those presented earlier by us (Brown & Noble, 1969a, b) and by Ojeda & Rougier (1974) are discussed."}
{"id": "PMID:1086357", "title": "Potassium accumulation and depletion in frog atrial muscle.", "content": "1. In atrial wall trabeculae of Rana catesbeiana and R. ridibunda very slowly decaying membrane currents have been consistently observed in decay tails following voltage clamp depolarizing and hyperpolarizing pulses. It is not thought that these currents are carried by time-dependent conductance channels but rather result from potassium ion accumulation or depletion. 2. Since voltage clamp techniques generally impose a non-physiological situation on the membranes of excitable cells, evidence that potassium ion accumulation occurs in unclamped atrial tissue is presented. 3. When potassium ions accumulate, the reversal potentials for both atrial delayed conductance mechanisms, ixfast and ixslow, should be shifted in a positive direction, the magnitude of the shifts being a function of the charge transferred during depolarization. Experiments have been performed to test this prediction quantitatively, and as a result, a simple accumulation model is developed. 4. A second important effect of accumulation should be upon the time-independent potassium conductance, iK1. It was found that this effect produces current tails whose decay becomes exponential when the amount of accumulation is small. The time constant of this exponential is shown to be equal to the time constant of decay of accumulation, tauacc. 5. One of the most important assumptions in the accumulation model is that the iK1(Em) relations for different values of [K]O 'cross-over' one another as they do in skeletal muscle and mammalian Purkinje tissue. Experimental verification of this assumption is presented. This 'cross-over' effect allows current changes due to accumulation to show an apparent 'reversal potential' and so to appear like a conductance mechanism. 6. Potassium depletion is shown to occur during hyperpolarizing pulses. This depletion process must be allowed for in a direct kinetic analysis of the pace-maker current, ixslow, at potentials negative to the resting potential (ER).", "contents": "Potassium accumulation and depletion in frog atrial muscle. 1. In atrial wall trabeculae of Rana catesbeiana and R. ridibunda very slowly decaying membrane currents have been consistently observed in decay tails following voltage clamp depolarizing and hyperpolarizing pulses. It is not thought that these currents are carried by time-dependent conductance channels but rather result from potassium ion accumulation or depletion. 2. Since voltage clamp techniques generally impose a non-physiological situation on the membranes of excitable cells, evidence that potassium ion accumulation occurs in unclamped atrial tissue is presented. 3. When potassium ions accumulate, the reversal potentials for both atrial delayed conductance mechanisms, ixfast and ixslow, should be shifted in a positive direction, the magnitude of the shifts being a function of the charge transferred during depolarization. Experiments have been performed to test this prediction quantitatively, and as a result, a simple accumulation model is developed. 4. A second important effect of accumulation should be upon the time-independent potassium conductance, iK1. It was found that this effect produces current tails whose decay becomes exponential when the amount of accumulation is small. The time constant of this exponential is shown to be equal to the time constant of decay of accumulation, tauacc. 5. One of the most important assumptions in the accumulation model is that the iK1(Em) relations for different values of [K]O 'cross-over' one another as they do in skeletal muscle and mammalian Purkinje tissue. Experimental verification of this assumption is presented. This 'cross-over' effect allows current changes due to accumulation to show an apparent 'reversal potential' and so to appear like a conductance mechanism. 6. Potassium depletion is shown to occur during hyperpolarizing pulses. This depletion process must be allowed for in a direct kinetic analysis of the pace-maker current, ixslow, at potentials negative to the resting potential (ER)."}
{"id": "PMID:1086358", "title": "The influence of non-uniformity on the analysis of potassium currents in heart muscle.", "content": "1. A method is described for determining the space constant gamma of heart muscle strips using a sucrose gap technique. 2. The average value of gamma for frog atrial trabeculae was found to be nearly 700 mum. This value is nearly twice the length of the test gap (400 mum). Near the resting potential, the voltage non-uniformity should be about 10%. This was confirmed experimentally by comparing the membrane voltages recorded across the current-passing and voltage-recording sucrose gaps. 3. The non-uniformity during large depolarizations was calculated using a computer model. This model includes the inward-going rectification displayed by iK1 and the delayed rectification that occurs following depolarizations beyond -40mV. A single component of delayed rectification was included. 4. It is shown that even very large non-uniformities have relatively small effects on the shape of the activation curve and on the time course of onset or decay of current. 5. It is comcluded that the fast component of current decay described in a previous paper (Brown, Clark & Noble, 1976b) is not attributable to a non-uniformity artifact.", "contents": "The influence of non-uniformity on the analysis of potassium currents in heart muscle. 1. A method is described for determining the space constant gamma of heart muscle strips using a sucrose gap technique. 2. The average value of gamma for frog atrial trabeculae was found to be nearly 700 mum. This value is nearly twice the length of the test gap (400 mum). Near the resting potential, the voltage non-uniformity should be about 10%. This was confirmed experimentally by comparing the membrane voltages recorded across the current-passing and voltage-recording sucrose gaps. 3. The non-uniformity during large depolarizations was calculated using a computer model. This model includes the inward-going rectification displayed by iK1 and the delayed rectification that occurs following depolarizations beyond -40mV. A single component of delayed rectification was included. 4. It is shown that even very large non-uniformities have relatively small effects on the shape of the activation curve and on the time course of onset or decay of current. 5. It is comcluded that the fast component of current decay described in a previous paper (Brown, Clark & Noble, 1976b) is not attributable to a non-uniformity artifact."}
{"id": "PMID:1086359", "title": "Noise analysis of drug induced voltage clamp currents in denervated frog muscle fibres.", "content": "1. Voltage clamp currents were recorded during iontophoretic application of steady doses of acetylcholine (ACh), carbachol or suberyldicholine to hyperpersensitive extrasynaptic regions of chronically denervated frog muscle fibers. Autocorrelation functions of drug induced current fluctuations were calculated and estimates of conductance gamma and average open time tau of the extrasynaptic ion channels were derived. 2. The average open time of an extrajunctional channel induced by acetylcholine is tauACh = 11 +/- 1-6 msec (+/- S.E.) at -80 mV and 8 degrees C. Carbachol and suberyldicholine open channels of tauCarb = 3-9 +/- 0-4 msec and tauSubCh = 19 +/- 2-5 msec (+/- S.E.) duration under the same conditions. The average open time of the extrasynaptic channel produced by each drug is three to five times longer than the value found for junctional channels in normal fibres. 3. The average open time of the extrajunctional channel is dependent on temperature and membrane potential. Lowering the temperature or increasing the membrane potential increases the average open time of the channels induced by any one of the drugs. 4. The conductance of a single extrajunctional channel opened by the action of acetylcholine is estimated to be gammaextra = 15 +/- 1-8 pmho (+/- S.E.). This is somewhat lower than the value of gammaep = 23 +/- 2 pmho (+/- S.E.) found for the conductance of a single open channel in the junctional membrane of normal fibres. The extrasynaptic channels opened by the action of carbachol and suberyldicholine have similar conductances to those produced by ACh. 5. The autocorrelation function of drug-induced current fluctuations, recorded at the former end-plate region of chronically denervated fibres often shows both a fast and a slow time constant. They correspond in value to the time constant of the autocorrelation function obtained from end-plate currents in normal fibres and from extrasynaptic currents in denervated fibres respectively. This could indicate that two populations of channels exist at the former end-plate region of denervated muscle fibres.", "contents": "Noise analysis of drug induced voltage clamp currents in denervated frog muscle fibres. 1. Voltage clamp currents were recorded during iontophoretic application of steady doses of acetylcholine (ACh), carbachol or suberyldicholine to hyperpersensitive extrasynaptic regions of chronically denervated frog muscle fibers. Autocorrelation functions of drug induced current fluctuations were calculated and estimates of conductance gamma and average open time tau of the extrasynaptic ion channels were derived. 2. The average open time of an extrajunctional channel induced by acetylcholine is tauACh = 11 +/- 1-6 msec (+/- S.E.) at -80 mV and 8 degrees C. Carbachol and suberyldicholine open channels of tauCarb = 3-9 +/- 0-4 msec and tauSubCh = 19 +/- 2-5 msec (+/- S.E.) duration under the same conditions. The average open time of the extrasynaptic channel produced by each drug is three to five times longer than the value found for junctional channels in normal fibres. 3. The average open time of the extrajunctional channel is dependent on temperature and membrane potential. Lowering the temperature or increasing the membrane potential increases the average open time of the channels induced by any one of the drugs. 4. The conductance of a single extrajunctional channel opened by the action of acetylcholine is estimated to be gammaextra = 15 +/- 1-8 pmho (+/- S.E.). This is somewhat lower than the value of gammaep = 23 +/- 2 pmho (+/- S.E.) found for the conductance of a single open channel in the junctional membrane of normal fibres. The extrasynaptic channels opened by the action of carbachol and suberyldicholine have similar conductances to those produced by ACh. 5. The autocorrelation function of drug-induced current fluctuations, recorded at the former end-plate region of chronically denervated fibres often shows both a fast and a slow time constant. They correspond in value to the time constant of the autocorrelation function obtained from end-plate currents in normal fibres and from extrasynaptic currents in denervated fibres respectively. This could indicate that two populations of channels exist at the former end-plate region of denervated muscle fibres."}
{"id": "PMID:1086361", "title": "Osmotic reflextion coefficients of capillary walls to low molecular weight hydrophilic solutes measured in single perfused capillaries of the frog mesentery.", "content": "1. Individual capillaries of the transilluminated frog mesentery have been perfused with suspensions of human red cells in frog Ringer solution containing 1-0 g albumin 100 ml.-1. The outer surface of the mesentery has been washed with normal frog Ringer solution and with frog Ringer solutions made hypertonic by addition of one of the following solutes: sodium chloride (100 m-mole. 1.-1); urea (100 m-mole.1.-1); sucrose (20-50 m-mole. 1.-1); cyanocobalamin (8-5 m-mole. 1.-1). The temperature of the mesentery was between 14 and 16 degrees C in all experiments. 2. Wtih the mesentery superfused with normal Ringer, the filtration coefficient was determined from measurements of the rate of fluid filtration across the capillary wall, at a series of known capillary pressures (Michel, Mason, Curry & Tooke, 1974). Filtration coefficient varied from 0-69 X 10(-3) to 4-45 X 10(-3) mum. sec-1 .cm H2O-1 with an average value of 1-87 X 10(-3) mum. sec-1. cm H2O-1. 3. When the superfusate was made hypertonic by the addition of a test solute, the osmotic reflextion coefficient (sigma) of the capillary wall to test solute was calculated from the additional rate of filtration, the concentration of test solute in the superfusate and the filtration coefficient. Average values for sigma were: sodium chloride, 0-068 +/- 0-03 (three capillaries); urea, 0-071 +/- 0.015 (four capillaries); sucrose, 0-115 +/- 0-023 (seven capillaries); cyanocobalamin, 0-100 +/- 0-03 (three capillaries). 4. In further experiments, the osmotic reflextion coefficients to sodium chloride, urea and sucrose were determined in the same capillary. Five technically acceptable experiments were carried out. Although there were differences in the value of sigma between different capillaries, in any one capillary values of sigma were of the same magnitude and there appeared to be no significant trend with the molecular size of the test solute. 5. Our findings are inconsistent with the hypothesis that there is a single pathway for water and small hydrophilic molecules across the capillary wall. 6. Our results may be interpreted in terms of an exclusive channel for water in parallel with a channel shared by both water and small hydrophilic molecules. It is suggested that the exclusive water channel may be the membranes and cytoplasm of the endothelial cells and the shared channel may be located in the intercellular junctions. 7. Our data suggest the exclusive water channel represents about 10% of the total filtration coefficient in frog mesenteric capillaries. The shared channel shows relatively little restriction to the molecules investigated. Estimates of the volume flow throught the two channels are made for conditions where hydrostatic pressure differences and osmotic pressure differences are the driving forces.", "contents": "Osmotic reflextion coefficients of capillary walls to low molecular weight hydrophilic solutes measured in single perfused capillaries of the frog mesentery. 1. Individual capillaries of the transilluminated frog mesentery have been perfused with suspensions of human red cells in frog Ringer solution containing 1-0 g albumin 100 ml.-1. The outer surface of the mesentery has been washed with normal frog Ringer solution and with frog Ringer solutions made hypertonic by addition of one of the following solutes: sodium chloride (100 m-mole. 1.-1); urea (100 m-mole.1.-1); sucrose (20-50 m-mole. 1.-1); cyanocobalamin (8-5 m-mole. 1.-1). The temperature of the mesentery was between 14 and 16 degrees C in all experiments. 2. Wtih the mesentery superfused with normal Ringer, the filtration coefficient was determined from measurements of the rate of fluid filtration across the capillary wall, at a series of known capillary pressures (Michel, Mason, Curry & Tooke, 1974). Filtration coefficient varied from 0-69 X 10(-3) to 4-45 X 10(-3) mum. sec-1 .cm H2O-1 with an average value of 1-87 X 10(-3) mum. sec-1. cm H2O-1. 3. When the superfusate was made hypertonic by the addition of a test solute, the osmotic reflextion coefficient (sigma) of the capillary wall to test solute was calculated from the additional rate of filtration, the concentration of test solute in the superfusate and the filtration coefficient. Average values for sigma were: sodium chloride, 0-068 +/- 0-03 (three capillaries); urea, 0-071 +/- 0.015 (four capillaries); sucrose, 0-115 +/- 0-023 (seven capillaries); cyanocobalamin, 0-100 +/- 0-03 (three capillaries). 4. In further experiments, the osmotic reflextion coefficients to sodium chloride, urea and sucrose were determined in the same capillary. Five technically acceptable experiments were carried out. Although there were differences in the value of sigma between different capillaries, in any one capillary values of sigma were of the same magnitude and there appeared to be no significant trend with the molecular size of the test solute. 5. Our findings are inconsistent with the hypothesis that there is a single pathway for water and small hydrophilic molecules across the capillary wall. 6. Our results may be interpreted in terms of an exclusive channel for water in parallel with a channel shared by both water and small hydrophilic molecules. It is suggested that the exclusive water channel may be the membranes and cytoplasm of the endothelial cells and the shared channel may be located in the intercellular junctions. 7. Our data suggest the exclusive water channel represents about 10% of the total filtration coefficient in frog mesenteric capillaries. The shared channel shows relatively little restriction to the molecules investigated. Estimates of the volume flow throught the two channels are made for conditions where hydrostatic pressure differences and osmotic pressure differences are the driving forces."}
{"id": "PMID:1086364", "title": "Pulmonary dysfunction in systemic lupus erythematosus without pulmonary symptoms.", "content": "Pulmonary function was measured in a group of 28 patients with systemic lupus erythematosus (SLE) who were free of pulmonary involvement at the time of the study. When compared with age and sex matched controls, the SLE patients had a pattern of restriction with reduced lung volumes and vital capacity. Diffusing capacity was reduced in proportion to the reduction in lung volume. This is felt to be most compatible with inapparent pleural thickening resulting in impaired lung expansion. There was no evidence of airway obstruction on maximal expiratory flow-volume curves. The effect of cigarette smoking in the SLE patients was a reduction in flows at low lung volumes, which was indistinguishable from the effects in the control smokers. Both SLE and control smokers had a significant reduction in DL/VA when compared with control nonsmokers. Pulmonary function was not influenced by the presence of renal disease.", "contents": "Pulmonary dysfunction in systemic lupus erythematosus without pulmonary symptoms. Pulmonary function was measured in a group of 28 patients with systemic lupus erythematosus (SLE) who were free of pulmonary involvement at the time of the study. When compared with age and sex matched controls, the SLE patients had a pattern of restriction with reduced lung volumes and vital capacity. Diffusing capacity was reduced in proportion to the reduction in lung volume. This is felt to be most compatible with inapparent pleural thickening resulting in impaired lung expansion. There was no evidence of airway obstruction on maximal expiratory flow-volume curves. The effect of cigarette smoking in the SLE patients was a reduction in flows at low lung volumes, which was indistinguishable from the effects in the control smokers. Both SLE and control smokers had a significant reduction in DL/VA when compared with control nonsmokers. Pulmonary function was not influenced by the presence of renal disease."}
{"id": "PMID:1086365", "title": "The insensitivity of frog heart rate to pulse modulated microwave energy.", "content": "Frey and Seifert (1968) have reported that pulse modulated microwave irradiation of low average energy density can produce tachycardia in isolated frog hearts. In an effort to examine this phenomenon, two types of experiments were carried out: 1. frog hearts were irradiated in situ with 100 mus bursts of microwave energy at either 10.0 GHz or 1.42 GHz; 2. isolated frog hearts were irradiated with 100 mus bursts of microwave energy at 1.42 GHz. No significant changes in heart rate were observed.", "contents": "The insensitivity of frog heart rate to pulse modulated microwave energy. Frey and Seifert (1968) have reported that pulse modulated microwave irradiation of low average energy density can produce tachycardia in isolated frog hearts. In an effort to examine this phenomenon, two types of experiments were carried out: 1. frog hearts were irradiated in situ with 100 mus bursts of microwave energy at either 10.0 GHz or 1.42 GHz; 2. isolated frog hearts were irradiated with 100 mus bursts of microwave energy at 1.42 GHz. No significant changes in heart rate were observed."}
{"id": "PMID:1086368", "title": "T- and B-lymphocytes in chronic lymphocytic leukemia: correlation with clinical and immunologic status of the disease.", "content": "T- and B-lymphocyte count were correlated with clinical and immunologic status of 59 patients with chronic lymphocytic leukemia. The mean total T-cell count was slightly lower than normal in patients in complete remission, within normal limits in those in partial remission, and significantly higher in those with active disease. The mean total B-cell count, however, was slightly elevated in patients in complete remission and those in partial remission, and markedly elevated in those with active disease. The T-cell count correlated well with the duration of disease in patients in remission: The mean count was within normal range in those patients with disease of less than 3 years, whereas for those patients with disease of 3-12 years, a significant reduction was observed. The T-cell count was well correlated with the status of skin test response of patients with either complete or partial remission; the B-cell count did not correlate with immunoglobulin levels in patients with this disease.", "contents": "T- and B-lymphocytes in chronic lymphocytic leukemia: correlation with clinical and immunologic status of the disease. T- and B-lymphocyte count were correlated with clinical and immunologic status of 59 patients with chronic lymphocytic leukemia. The mean total T-cell count was slightly lower than normal in patients in complete remission, within normal limits in those in partial remission, and significantly higher in those with active disease. The mean total B-cell count, however, was slightly elevated in patients in complete remission and those in partial remission, and markedly elevated in those with active disease. The T-cell count correlated well with the duration of disease in patients in remission: The mean count was within normal range in those patients with disease of less than 3 years, whereas for those patients with disease of 3-12 years, a significant reduction was observed. The T-cell count was well correlated with the status of skin test response of patients with either complete or partial remission; the B-cell count did not correlate with immunoglobulin levels in patients with this disease."}
{"id": "PMID:1086369", "title": "Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus.", "content": "A restriction endonuclease from Haemophilus influenzae (Hind III) specifically cleaved vaccinia DNA into 14 fragments. The molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). Hind III digestion of the DNA from the WR and CV-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. The average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6).", "contents": "Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus. A restriction endonuclease from Haemophilus influenzae (Hind III) specifically cleaved vaccinia DNA into 14 fragments. The molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). Hind III digestion of the DNA from the WR and CV-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. The average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6)."}
{"id": "PMID:1086370", "title": "Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures.", "content": "Influenza viral complementary RNA (cRNA), i.e., viral mRNA was radioactive when purified from the cytoplasmic fraction of cordycepin-treated canine kidney cells that were incubated with [methyl-3H]methionine during infection. Approximately 55 to 60% of the methyl-3H radioactivity was in internal N6-methyladenosine, a feature distinguishing this mRNA from those viral mRNA's that are known to be synthesized in the cytoplasm. The remaining methyl-3H radioactivity was in 5'-terminal cap structures that consisted of 7-methylguanosine in pyrophosphate linkage to 2'-o-methyladenosine, N6, 2'-O-dimethyladenosine, or 2'-O-methylguanosine. Methylated adenosine was the predominant penultimate nucleoside in caps, suggesting that cRNA synthesis in infected cells initiates preferentially with adenosine at the 5' end. In contrast to cRNA, influenza virion RNA segments extracted from purified virus contained mainly 5'-terminal ppA and no detectable cap structures.", "contents": "Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures. Influenza viral complementary RNA (cRNA), i.e., viral mRNA was radioactive when purified from the cytoplasmic fraction of cordycepin-treated canine kidney cells that were incubated with [methyl-3H]methionine during infection. Approximately 55 to 60% of the methyl-3H radioactivity was in internal N6-methyladenosine, a feature distinguishing this mRNA from those viral mRNA's that are known to be synthesized in the cytoplasm. The remaining methyl-3H radioactivity was in 5'-terminal cap structures that consisted of 7-methylguanosine in pyrophosphate linkage to 2'-o-methyladenosine, N6, 2'-O-dimethyladenosine, or 2'-O-methylguanosine. Methylated adenosine was the predominant penultimate nucleoside in caps, suggesting that cRNA synthesis in infected cells initiates preferentially with adenosine at the 5' end. In contrast to cRNA, influenza virion RNA segments extracted from purified virus contained mainly 5'-terminal ppA and no detectable cap structures."}
{"id": "PMID:1086371", "title": "Chronic painless hematuria and urethral bleeding as the presenting manifestations of Osler-Weber-Rendu disease.", "content": "A case of a young man with profound iron deficiency anemia, chronic painless hematuria and urethral bleeding is presented. He had the typical cutaneous findings of Osler-Weber-Rendu disease (hereditary hemorrhagic telangiectasia). Appropriate urological studies demonstrated bladder and urethral telangiectases, with no other explanation for the bleeding. Osler-Weber-Rendu disease should be considered in the differential diagnosis of chronic painless hematuria.", "contents": "Chronic painless hematuria and urethral bleeding as the presenting manifestations of Osler-Weber-Rendu disease. A case of a young man with profound iron deficiency anemia, chronic painless hematuria and urethral bleeding is presented. He had the typical cutaneous findings of Osler-Weber-Rendu disease (hereditary hemorrhagic telangiectasia). Appropriate urological studies demonstrated bladder and urethral telangiectases, with no other explanation for the bleeding. Osler-Weber-Rendu disease should be considered in the differential diagnosis of chronic painless hematuria."}
{"id": "PMID:1086373", "title": "Endoscopic electrocoagulation of upper gastrointestinal hemorrhage.", "content": "Endoscopic electrocoagulation was performed on 40 occasions for 38 patients with bleeding gastrointestinal lesions. Cessation of bleeding was achieved in 95%. Fifteen gastric ulcers, 14 duodenal ulcers, six Mallory-Weiss tears, one gastric varix, one hemorrhagic antral gastritis, and one esophageal ulcer were successfully electrocoagulated. Three duodenal and three gastric ulcers rebled. One duodenal ulcer and one gastric ulcer were successfully reelectrocoagulated. Failure to stop bleeding by electrocoagulation occurred in one Mallory-Weiss tear and one duodenal ulcer. There was no morbidity nor mortality attributed to endoscopic electrocoagulation. A retrospective cost analysis showed that the cost of hospitalization was less in patients treated by electrocoagulation. Patients so treated were hospitalized for a shorter duration.", "contents": "Endoscopic electrocoagulation of upper gastrointestinal hemorrhage. Endoscopic electrocoagulation was performed on 40 occasions for 38 patients with bleeding gastrointestinal lesions. Cessation of bleeding was achieved in 95%. Fifteen gastric ulcers, 14 duodenal ulcers, six Mallory-Weiss tears, one gastric varix, one hemorrhagic antral gastritis, and one esophageal ulcer were successfully electrocoagulated. Three duodenal and three gastric ulcers rebled. One duodenal ulcer and one gastric ulcer were successfully reelectrocoagulated. Failure to stop bleeding by electrocoagulation occurred in one Mallory-Weiss tear and one duodenal ulcer. There was no morbidity nor mortality attributed to endoscopic electrocoagulation. A retrospective cost analysis showed that the cost of hospitalization was less in patients treated by electrocoagulation. Patients so treated were hospitalized for a shorter duration."}
{"id": "PMID:1086384", "title": "Effects of BCG (Bacillus Calmette-Gu\u00e9rin) vaccines on immune responses in mice. I. Possible effect of BCG on helper T cells.", "content": "The effects of killed and living BCG on antibody production against hamster erythrocytes (HRBC) and the 2, 4, 6-trinitrophenyl (TNP) group were studied in SL mice. Killed and living BCG, each in doses of 0.008 mg, 0.08 mg, 0.8 mg and 8 mg per mouse, were intravenously inoculated 7 days prior to primary immunization with HRBC. Secondary immunization was carried out 28 days later with TNP-HRBC. Anti-HRBC and anti-TNP antibodies were estimated by a hemagglutination test. The results showed that pretreatment with killed or living BCG enhanced the antibody production against both HRBC and TNP. Comparing the effects of these two BCG preparations, it was noted that killed BCG augmented the anti-HRBC antibody production more effectively than living BCG. In regard to the anti-TNP antibody production, living BCG exhibited a greater augmenting effect than killed BCG. This difference in the modes of action of killed and living BCG was remarkable when two groups given 8 mg of killed and living BCG were compared. In addition, it was shown that living BCG at a dose as high as 8 mg was able to augment the anti-TNP antibody production, even in the absence of preceding immunization with HRBC.", "contents": "Effects of BCG (Bacillus Calmette-Gu\u00e9rin) vaccines on immune responses in mice. I. Possible effect of BCG on helper T cells. The effects of killed and living BCG on antibody production against hamster erythrocytes (HRBC) and the 2, 4, 6-trinitrophenyl (TNP) group were studied in SL mice. Killed and living BCG, each in doses of 0.008 mg, 0.08 mg, 0.8 mg and 8 mg per mouse, were intravenously inoculated 7 days prior to primary immunization with HRBC. Secondary immunization was carried out 28 days later with TNP-HRBC. Anti-HRBC and anti-TNP antibodies were estimated by a hemagglutination test. The results showed that pretreatment with killed or living BCG enhanced the antibody production against both HRBC and TNP. Comparing the effects of these two BCG preparations, it was noted that killed BCG augmented the anti-HRBC antibody production more effectively than living BCG. In regard to the anti-TNP antibody production, living BCG exhibited a greater augmenting effect than killed BCG. This difference in the modes of action of killed and living BCG was remarkable when two groups given 8 mg of killed and living BCG were compared. In addition, it was shown that living BCG at a dose as high as 8 mg was able to augment the anti-TNP antibody production, even in the absence of preceding immunization with HRBC."}
{"id": "PMID:1086396", "title": "[Preinfarct stenocardia and acute myocardial infarct from the viewpoint of modern coronary surgery].", "content": "The results of aorto-coronary shunting in 36 patients with preinfarction angina and of urgent direct myocardial revascularization in 17 patients with acute myocardial infarction are presented. The surgical mortality comprised 28% in the group of patients with preinfarction angina. Twenty-two patients were followed-up for 8 months to 4 years. Good results were obtained in 9 patients, satisfactory--in 7, unsatisfactory--in 4. One patient died of cardiac insufficiency 1 1/2 year after surgery. The diagnosis of preinfarction angina is a direct indication for urgent coronary angiography and aorto-coronary shunting in case suitable coronary arteries are available for anastomosing. The indications for urgent revascularization of the myocardium in cases of infarction included the inefficacy of drug therapy within 2-3 hours of its onset, an unarrested pulmonary oedema and cardiogenic shock in cases of localized proximal occlusion of the coronaries revealed by elective or urgent coronary angiography. Four patients were operated on in the state of cardiogenic shock (one of them after reversing the state of clinical death), and two patients were operated on with pulmonary oedema. All these patients (with the exception of the one with pulmonary oedema) recovered. Four patients died. The follow-up covered a period of 9 months to 3 years. Good results were obtained in 4 patients, satisfactory--in 3, unsatisfactory--in 4. No late mortality cases were observed. The obtained results permit to analyse the preinfarction angina and acute myocardial infarction from the standpoint of modern coronary surgery.", "contents": "[Preinfarct stenocardia and acute myocardial infarct from the viewpoint of modern coronary surgery]. The results of aorto-coronary shunting in 36 patients with preinfarction angina and of urgent direct myocardial revascularization in 17 patients with acute myocardial infarction are presented. The surgical mortality comprised 28% in the group of patients with preinfarction angina. Twenty-two patients were followed-up for 8 months to 4 years. Good results were obtained in 9 patients, satisfactory--in 7, unsatisfactory--in 4. One patient died of cardiac insufficiency 1 1/2 year after surgery. The diagnosis of preinfarction angina is a direct indication for urgent coronary angiography and aorto-coronary shunting in case suitable coronary arteries are available for anastomosing. The indications for urgent revascularization of the myocardium in cases of infarction included the inefficacy of drug therapy within 2-3 hours of its onset, an unarrested pulmonary oedema and cardiogenic shock in cases of localized proximal occlusion of the coronaries revealed by elective or urgent coronary angiography. Four patients were operated on in the state of cardiogenic shock (one of them after reversing the state of clinical death), and two patients were operated on with pulmonary oedema. All these patients (with the exception of the one with pulmonary oedema) recovered. Four patients died. The follow-up covered a period of 9 months to 3 years. Good results were obtained in 4 patients, satisfactory--in 3, unsatisfactory--in 4. No late mortality cases were observed. The obtained results permit to analyse the preinfarction angina and acute myocardial infarction from the standpoint of modern coronary surgery."}
{"id": "PMID:1086400", "title": "[The phenotype of heterozygotic females in congenital red-green color blindness (author's transl)].", "content": "Varying degrees of deficient color perception along the Deutan axis within one family have been studied and presented. All seven family members exhibit manifest color blindness. The question of the phenotype among heterozygotic females is discussed on the basis of the data found by anomaloscope testing and the hue tests of Farnsworth D-15 and Farnsworth 100.", "contents": "[The phenotype of heterozygotic females in congenital red-green color blindness (author's transl)]. Varying degrees of deficient color perception along the Deutan axis within one family have been studied and presented. All seven family members exhibit manifest color blindness. The question of the phenotype among heterozygotic females is discussed on the basis of the data found by anomaloscope testing and the hue tests of Farnsworth D-15 and Farnsworth 100."}
{"id": "PMID:1086401", "title": "[Aphakia and contact lenses (author's transl)].", "content": "Four possibilities are offered to provide a good correction for aphakic individuals: no correction at all; spectacles; more than 60% adopt this solution, the contact lens which has optical, functional and aesthetic advantages. The intra-ocular lens (the best solution, but it is, not without complications). Since 1956 we have followed up 588 unilateral aphakians. Tolerance of contact lenses after one year is excellent: 61% wear the contact lenses 10-12 hrs. per day, 18% wear them 6-8 hrs. per day and 21% lost courage. There is a parallel between binocular vision and tolerance, which decreased over the years. Three factors are determinant here: state of binocular vision: many factors influence the quality of fusion: nature of the cataract, the moment of adaptation of the lens, loss of accommodation and anisoconie. Surrounding conditions e.g. dusty atmosphere also influence the tolerance; local state of the tissues. 15 years later, the situation has changed. 71.5% gave up the contact lenses completely and only 15% wear them 10-12 hrs. per day. The reasons of this abandonment are: diplopie, inflammation and local complications.", "contents": "[Aphakia and contact lenses (author's transl)]. Four possibilities are offered to provide a good correction for aphakic individuals: no correction at all; spectacles; more than 60% adopt this solution, the contact lens which has optical, functional and aesthetic advantages. The intra-ocular lens (the best solution, but it is, not without complications). Since 1956 we have followed up 588 unilateral aphakians. Tolerance of contact lenses after one year is excellent: 61% wear the contact lenses 10-12 hrs. per day, 18% wear them 6-8 hrs. per day and 21% lost courage. There is a parallel between binocular vision and tolerance, which decreased over the years. Three factors are determinant here: state of binocular vision: many factors influence the quality of fusion: nature of the cataract, the moment of adaptation of the lens, loss of accommodation and anisoconie. Surrounding conditions e.g. dusty atmosphere also influence the tolerance; local state of the tissues. 15 years later, the situation has changed. 71.5% gave up the contact lenses completely and only 15% wear them 10-12 hrs. per day. The reasons of this abandonment are: diplopie, inflammation and local complications."}
{"id": "PMID:1086403", "title": "[Diagnosis of Pneumocystic carinii pneumonia by lavage of the bronchial system (author's transl)].", "content": "This report deals with a 10 year old girl suffering from an acute lymphoblastic leukemia since 1971. The child was hematologically in full remission, when she developed a pneumonia due to pneumocystis carinii. The diagnosis was established by demonstrating the agent in bronchial secretions. The material was obtained by rinsing the bronchial system. The patient was treated with pentamidine and co-trimoxazol (Bactrim) with good success.", "contents": "[Diagnosis of Pneumocystic carinii pneumonia by lavage of the bronchial system (author's transl)]. This report deals with a 10 year old girl suffering from an acute lymphoblastic leukemia since 1971. The child was hematologically in full remission, when she developed a pneumonia due to pneumocystis carinii. The diagnosis was established by demonstrating the agent in bronchial secretions. The material was obtained by rinsing the bronchial system. The patient was treated with pentamidine and co-trimoxazol (Bactrim) with good success."}
{"id": "PMID:1086404", "title": "[T-cells in thyroid disease (author's transl)].", "content": "Thymus-derived peripheral blood lymphocytes were studied in untreated (n = 18), methimazole-treated (n = 28) thyrotoxicosis, after radioiodine (n = 14), in Hashimoto thyroiditis (n = 7) and in euthyroid goiter (n = 7). The results were compared with normal persons (n = 40) without thyroid disease. There was no significant difference in the total and relative counts of T-cells either between the different groups nor compared with the controls. These findings confirm that T-cells in peripheral blood cannot give any information about activity or prognosis of the various thyroid diseases. Thus T-cells give no further suggestion concerning the possible pathogenetic role of cell-mediated-immunity.", "contents": "[T-cells in thyroid disease (author's transl)]. Thymus-derived peripheral blood lymphocytes were studied in untreated (n = 18), methimazole-treated (n = 28) thyrotoxicosis, after radioiodine (n = 14), in Hashimoto thyroiditis (n = 7) and in euthyroid goiter (n = 7). The results were compared with normal persons (n = 40) without thyroid disease. There was no significant difference in the total and relative counts of T-cells either between the different groups nor compared with the controls. These findings confirm that T-cells in peripheral blood cannot give any information about activity or prognosis of the various thyroid diseases. Thus T-cells give no further suggestion concerning the possible pathogenetic role of cell-mediated-immunity."}
{"id": "PMID:1086408", "title": "Sternal and costochondral infections following open-heart surgery. A review of 2,594 cases.", "content": "From a series of 2,594 patients undergoing open-heart surgery, 39 had sternal or costochondral infections. Most of these infections were associated with a number of predisposing factors: prolonged perfusion time, excessive postoperative bleeding, depressed cardiac output in the postoperative period, and a history of re-exploration for the control of hemorrhage. One important factor was the use of bilateral internal mammary artery implants. The prognosis for patients with sternal wound infections appears related to the length of time required for institution of treatment and the adequacy of initial therapy. Most of these infections are caused by staphylococcus, although the more complicated infections often are caused by fungus. The prevention of serious sternal infections depends on a combination of proper preoperative preparation, attention to minute details at the time of operation, and recognition of variables predisposing to wound complications.", "contents": "Sternal and costochondral infections following open-heart surgery. A review of 2,594 cases. From a series of 2,594 patients undergoing open-heart surgery, 39 had sternal or costochondral infections. Most of these infections were associated with a number of predisposing factors: prolonged perfusion time, excessive postoperative bleeding, depressed cardiac output in the postoperative period, and a history of re-exploration for the control of hemorrhage. One important factor was the use of bilateral internal mammary artery implants. The prognosis for patients with sternal wound infections appears related to the length of time required for institution of treatment and the adequacy of initial therapy. Most of these infections are caused by staphylococcus, although the more complicated infections often are caused by fungus. The prevention of serious sternal infections depends on a combination of proper preoperative preparation, attention to minute details at the time of operation, and recognition of variables predisposing to wound complications."}
{"id": "PMID:1086409", "title": "Preoperative risk factors and aorta-coronary bypass graft patency.", "content": "The relationship between graft patency and the presence of presurgical prognostic factors was studied in 636 patients who have undergone aorta-coronary bypass operation. We have found a positive correlation between vein graft patency and both coronary artery constriction score (p less than 0.01) and diastolic blood pressure (p less than 0.05). In patients studied up to one year after surgery, high graft patency was associated with both higher diastolic pressures (p less than 0.03) and higher cholesterol levels (p less than 0.001). However, high cholesterol levels also were associated with coronary arterial obstruction. In patients studied more than a year after operation, high constriction scores before the operation were associated with improved graft patency (p less than 0.03). Our findings indicate that short-term prognosis of the grafts is related mainly to hemodynamic variables.", "contents": "Preoperative risk factors and aorta-coronary bypass graft patency. The relationship between graft patency and the presence of presurgical prognostic factors was studied in 636 patients who have undergone aorta-coronary bypass operation. We have found a positive correlation between vein graft patency and both coronary artery constriction score (p less than 0.01) and diastolic blood pressure (p less than 0.05). In patients studied up to one year after surgery, high graft patency was associated with both higher diastolic pressures (p less than 0.03) and higher cholesterol levels (p less than 0.001). However, high cholesterol levels also were associated with coronary arterial obstruction. In patients studied more than a year after operation, high constriction scores before the operation were associated with improved graft patency (p less than 0.03). Our findings indicate that short-term prognosis of the grafts is related mainly to hemodynamic variables."}
{"id": "PMID:1086413", "title": "Total pancreatectomy in the treatment of acute necrotising and hemorrhagic pancreatitis. Indications-technique.", "content": "The place of total pancreatectomy in the treatment of pancreatitis is still not clear: the author is in favour of this operation and gives the indications, surgical technique, complications and results. The operation is indicated in cases of necrosis involving more than 2/3rds of the whole of the head and part of the body of the pancreas. The duodenum and pancreas should be removed in one piece and intestinal continuity should be restored performing choledocho-jejunal and gastro-jejunal anastomoses. It is important to carry out this operation early, between the 3rd and 6th days, treating all areas  of necrosis before the lesions become the site of uncontrollable infection. Seven patients out of 9 are still alive, on the 25th of July 1975; they all have easily controlled diabetes, a low fat diet and are receiving pancreatic extract. We have recently operated a 10th case, and the patient is alive 2 months later.", "contents": "Total pancreatectomy in the treatment of acute necrotising and hemorrhagic pancreatitis. Indications-technique. The place of total pancreatectomy in the treatment of pancreatitis is still not clear: the author is in favour of this operation and gives the indications, surgical technique, complications and results. The operation is indicated in cases of necrosis involving more than 2/3rds of the whole of the head and part of the body of the pancreas. The duodenum and pancreas should be removed in one piece and intestinal continuity should be restored performing choledocho-jejunal and gastro-jejunal anastomoses. It is important to carry out this operation early, between the 3rd and 6th days, treating all areas  of necrosis before the lesions become the site of uncontrollable infection. Seven patients out of 9 are still alive, on the 25th of July 1975; they all have easily controlled diabetes, a low fat diet and are receiving pancreatic extract. We have recently operated a 10th case, and the patient is alive 2 months later."}
{"id": "PMID:1086414", "title": "[Experimental pulmonary edema: pathophysiological mechanism (author's transl)].", "content": "Traumaticed tissue or organs show different reactions to oral and parenteral application of watery solutions. Tissue traumaticed or not does incline to edema. 2. In its central position in blood circulation, its capability of water storage and distribution as well in its regulatory function of the cardiac output, the lung is very suitable for transportation of fluid. 3. If physiologic transportation exceeds a maximum (renal and cardiac insufficiency, high temperature, infusion) and if lymphatics are blocked, edema results in the interstitial and intraalveolare space. 4. Fatal edema is prevented during the first 48 h by applicating proteinase inhibitor drug; Trasylol immediatly after trauma. 5. Edema is significantly diminished applicating Benzopyrone (Venalot), as proven biochemically and electromicroscopically. 6. Taking the findings, it is necessary to considerate a new concept of fluid therapy.", "contents": "[Experimental pulmonary edema: pathophysiological mechanism (author's transl)]. Traumaticed tissue or organs show different reactions to oral and parenteral application of watery solutions. Tissue traumaticed or not does incline to edema. 2. In its central position in blood circulation, its capability of water storage and distribution as well in its regulatory function of the cardiac output, the lung is very suitable for transportation of fluid. 3. If physiologic transportation exceeds a maximum (renal and cardiac insufficiency, high temperature, infusion) and if lymphatics are blocked, edema results in the interstitial and intraalveolare space. 4. Fatal edema is prevented during the first 48 h by applicating proteinase inhibitor drug; Trasylol immediatly after trauma. 5. Edema is significantly diminished applicating Benzopyrone (Venalot), as proven biochemically and electromicroscopically. 6. Taking the findings, it is necessary to considerate a new concept of fluid therapy."}
{"id": "PMID:1086415", "title": "[Reflux esophagitis (author's transl)].", "content": "The term \"Refluxdisease\" consider an esophageal response (either subjective or objective) to injury caused by gastrointestinal contents. Thickening of the basal layer of the esophageal mucosa, reflux esophagitis, endobrachyesophagus, acquired short esophagus and peptic stenosis are different stages of the objective esophageal response. The diagnosis of reflux disease must take account of these findings where appropriated. The various stages of reflux disease can only by recognized and adequately treated following an exact diagnosis that takes into account as may parameters as possible. For this reason fundoplication, which is at present the most effective procedure, should only be performed when the indications are particularly strong.", "contents": "[Reflux esophagitis (author's transl)]. The term \"Refluxdisease\" consider an esophageal response (either subjective or objective) to injury caused by gastrointestinal contents. Thickening of the basal layer of the esophageal mucosa, reflux esophagitis, endobrachyesophagus, acquired short esophagus and peptic stenosis are different stages of the objective esophageal response. The diagnosis of reflux disease must take account of these findings where appropriated. The various stages of reflux disease can only by recognized and adequately treated following an exact diagnosis that takes into account as may parameters as possible. For this reason fundoplication, which is at present the most effective procedure, should only be performed when the indications are particularly strong."}
{"id": "PMID:1086410", "title": "[Immunological mother-fetus relationship].", "content": "The immunology of propagation and growth is a field of medicine which has been developing in the last 3--4 years. On the basis of the literature data on clinical and experimental works from this field the author tries to explain the essence of the immunological mother-fetus relationship. The non-rejection of the fetus as a foreign transplant is made possible by the suppression of the mother's immunologic capacity (cellular and humoral) on the one hand, and the existence of protective mechanisms of the fetus on the other. Dealing in more detail with elements resulting in the reduced immunological reactivity and the increased tolerance of mother towards fetus, the author concludes that this increased tolerance, along with the existence of the intact placental barrier, is the basic factor which allows the survival of the fetus as an allotransplant.", "contents": "[Immunological mother-fetus relationship]. The immunology of propagation and growth is a field of medicine which has been developing in the last 3--4 years. On the basis of the literature data on clinical and experimental works from this field the author tries to explain the essence of the immunological mother-fetus relationship. The non-rejection of the fetus as a foreign transplant is made possible by the suppression of the mother's immunologic capacity (cellular and humoral) on the one hand, and the existence of protective mechanisms of the fetus on the other. Dealing in more detail with elements resulting in the reduced immunological reactivity and the increased tolerance of mother towards fetus, the author concludes that this increased tolerance, along with the existence of the intact placental barrier, is the basic factor which allows the survival of the fetus as an allotransplant."}
{"id": "PMID:1086420", "title": "Perioperative myocardial infarction with aortocoronary bypass graft surgery.", "content": "In 1975, 160 patients underwent aortocoronary bypass graft surgery at St. Vincent's Hospital, Sydney. The incidence of perioperative myocardial infarction varied between 2-5% and 5-6% depending on the criteria used to diagnose pathological Q waves, and thus compared favourably with other reported series. The low incidence suggested that perioperative infarction should be considered as neither a deterrent to coronary bypass surgery nor a significant cause of postoperative relief of angina.", "contents": "Perioperative myocardial infarction with aortocoronary bypass graft surgery. In 1975, 160 patients underwent aortocoronary bypass graft surgery at St. Vincent's Hospital, Sydney. The incidence of perioperative myocardial infarction varied between 2-5% and 5-6% depending on the criteria used to diagnose pathological Q waves, and thus compared favourably with other reported series. The low incidence suggested that perioperative infarction should be considered as neither a deterrent to coronary bypass surgery nor a significant cause of postoperative relief of angina."}
{"id": "PMID:1086421", "title": "Upper gastrointestinal endoscopy in surgical practice.", "content": "The use of fiberoptic endoscopy provides an accurate diagnosis in more than 90% of patients with lesions in the oesophagus, stomach and proximal duodenum. It is, however, an expensive and time-consuming investigation and is not always necessary when a satisfactory diagnosis has been made after radiological examination. Groups of patients have now been identified in which endoscopy is of particular value to the surgeon. When there is doubt about the diagnosis after radiological examination, particularly regarding the malignancy of a gastric ulcer, when an upper gastrointestinal haemorrhage occurs, when postgastrectomy patients present with symptoms, and when persistent symptoms occur in the absence of radiological findings, endoscopy is mandatory for an accurate diagnosis to be achieved and rational treatment instituted.", "contents": "Upper gastrointestinal endoscopy in surgical practice. The use of fiberoptic endoscopy provides an accurate diagnosis in more than 90% of patients with lesions in the oesophagus, stomach and proximal duodenum. It is, however, an expensive and time-consuming investigation and is not always necessary when a satisfactory diagnosis has been made after radiological examination. Groups of patients have now been identified in which endoscopy is of particular value to the surgeon. When there is doubt about the diagnosis after radiological examination, particularly regarding the malignancy of a gastric ulcer, when an upper gastrointestinal haemorrhage occurs, when postgastrectomy patients present with symptoms, and when persistent symptoms occur in the absence of radiological findings, endoscopy is mandatory for an accurate diagnosis to be achieved and rational treatment instituted."}
{"id": "PMID:1086422", "title": "[Experiences with enterally and parenterally applied leucovorin following therapy with high doses of methotrexate in malignant tumors (author's transl)].", "content": "45 patients (43 ovarian carcinoma and 2 chorionepithelioma) were treated with high doses of methotrexate and intramuscularly application of leucovorin according to Math\u00e9 et al. In comparison a group of 19 patients (ovarian carcinoma only) was treated with methotrexate and leucovorin-tablets. The toxic effects on leucocytes and platelets were equal in both groups. Due to slightly different doses of leucovorin in both groups an enteral resorption rate of more than 90 p.c. is calculated. Because of the simple way of application the suggested high-dose therapy with methotrexate and leucovorin-tablets seems to be most convenient in the moment.", "contents": "[Experiences with enterally and parenterally applied leucovorin following therapy with high doses of methotrexate in malignant tumors (author's transl)]. 45 patients (43 ovarian carcinoma and 2 chorionepithelioma) were treated with high doses of methotrexate and intramuscularly application of leucovorin according to Math\u00e9 et al. In comparison a group of 19 patients (ovarian carcinoma only) was treated with methotrexate and leucovorin-tablets. The toxic effects on leucocytes and platelets were equal in both groups. Due to slightly different doses of leucovorin in both groups an enteral resorption rate of more than 90 p.c. is calculated. Because of the simple way of application the suggested high-dose therapy with methotrexate and leucovorin-tablets seems to be most convenient in the moment."}
{"id": "PMID:1086423", "title": "High dose methotrexate therapy of solid tumors: observations relating to clinical toxicity.", "content": "In clinical studies performed during 111 infusions of high dose methotrexate (MTX) we have evolved a clinical and laboratory protocol which permits such therapy without prohibitive risk to the patient. The plasma MTX data obtained indicate that pharmacokinetic disposition is dose related during these infusions and that such data are useful in identifying patients at risk from serious toxicity.", "contents": "High dose methotrexate therapy of solid tumors: observations relating to clinical toxicity. In clinical studies performed during 111 infusions of high dose methotrexate (MTX) we have evolved a clinical and laboratory protocol which permits such therapy without prohibitive risk to the patient. The plasma MTX data obtained indicate that pharmacokinetic disposition is dose related during these infusions and that such data are useful in identifying patients at risk from serious toxicity."}
{"id": "PMID:1086418", "title": "[para-Aminobenzoic acid as a sole source of carbon and energy for Pseudomonas desmoliticum].", "content": "A strain of Psuedomonas desmoliticum has been isolated from soil. It utilizes, as a source of carbon and energy, p-aminobenozic (PABA), p-fluorobenzoic acids, and some other aromatic compounds. The strain has been isolated by inoculating soil suspensions onto Petri plates with a solid mineral medium containing 0.1% PABA as a carbon source. The preparatory metabolism of PABA was studied in this work; p-hydroxybenzoic and protocatechuic acids were found to be its intermediate products. Enzyme systems catalysing oxidation of aromatic compounds and glucose are inducible.", "contents": "[para-Aminobenzoic acid as a sole source of carbon and energy for Pseudomonas desmoliticum]. A strain of Psuedomonas desmoliticum has been isolated from soil. It utilizes, as a source of carbon and energy, p-aminobenozic (PABA), p-fluorobenzoic acids, and some other aromatic compounds. The strain has been isolated by inoculating soil suspensions onto Petri plates with a solid mineral medium containing 0.1% PABA as a carbon source. The preparatory metabolism of PABA was studied in this work; p-hydroxybenzoic and protocatechuic acids were found to be its intermediate products. Enzyme systems catalysing oxidation of aromatic compounds and glucose are inducible."}
{"id": "PMID:1086425", "title": "Effects of various drugs supposed to interact with serotonin on PGO frequency changes induced by reserpine and 5-hydroxytryptophan.", "content": "Reserpine induced ponto-geniculo-occipital (PGO) spikes in the lateral geniculate nucleus. This was used as a model to evaluate the possible anti-reserpine effects of Org GB 94-1,2,3,4,10,14b-hexahydro-2-methyldibenzo[c,f]pyrazino [1,2-alpha]azepine monohydrochloride-methiothepin, imipramine and atropine. In cats, under local anaesthesia, 3.2 mg/kg of methiothepin (a serotonin antagonist and neuroleptic) increased PGO density while 5-hydroxytryptophan (5-HTP) effects were blocked. A dose of 0.32 mg/kg of imipramine or atropine greatly reduced PGO density while potentiating 5-HTP effects. Org GB 94 had no effect in doses up to 10 mg/kg, where a slight decrease of PGO density was found, while 5-HTP effects were unchanged. At 32 mg/kg, EEG was pronouncedly changed by Org GB 94 but only a short-lasting decrease in PGO density was noted. On the basis of these results it seems improbable that the behavioural effects of Org GB 94 are to be explained by the same mechanism (i.e. serotonin uptake inhibition) as that of imipramine.", "contents": "Effects of various drugs supposed to interact with serotonin on PGO frequency changes induced by reserpine and 5-hydroxytryptophan. Reserpine induced ponto-geniculo-occipital (PGO) spikes in the lateral geniculate nucleus. This was used as a model to evaluate the possible anti-reserpine effects of Org GB 94-1,2,3,4,10,14b-hexahydro-2-methyldibenzo[c,f]pyrazino [1,2-alpha]azepine monohydrochloride-methiothepin, imipramine and atropine. In cats, under local anaesthesia, 3.2 mg/kg of methiothepin (a serotonin antagonist and neuroleptic) increased PGO density while 5-hydroxytryptophan (5-HTP) effects were blocked. A dose of 0.32 mg/kg of imipramine or atropine greatly reduced PGO density while potentiating 5-HTP effects. Org GB 94 had no effect in doses up to 10 mg/kg, where a slight decrease of PGO density was found, while 5-HTP effects were unchanged. At 32 mg/kg, EEG was pronouncedly changed by Org GB 94 but only a short-lasting decrease in PGO density was noted. On the basis of these results it seems improbable that the behavioural effects of Org GB 94 are to be explained by the same mechanism (i.e. serotonin uptake inhibition) as that of imipramine."}
{"id": "PMID:1086428", "title": "Selective and total shunts in the treatment of bleeding varices. A randomized controlled trial.", "content": "Two types of surgical therapy of bleeding esophageal varices were evaluated in 48 patients by a randomized controlled trial: 24 were randomized for a total shunt and 24 for the selective shunt. In two of the latter, a total shunt had to be performed for technical reasons. The fatality rates (six in the 24 total, and six in 22 selective [performed], and seven in 24 selective [randomized]), the frequency of shunt occlusion (two in each group), and of recurrent gastronintestinal bleeding (three in each group) were similar. Encephalopathy developed more often after a total shunt -- 10 of 24, or one per 58 patient-months -- than after selective (performed) -- one of 22, or one per 593 patient-months (P less than 0.005). Total shunts consistently diverted the hepatopetal mesenteric-portal flow from the liver. Deterioration of hepatic function (maximum rate of urea synthesis) was greater after total than selective shunt (P less than 0.05).", "contents": "Selective and total shunts in the treatment of bleeding varices. A randomized controlled trial. Two types of surgical therapy of bleeding esophageal varices were evaluated in 48 patients by a randomized controlled trial: 24 were randomized for a total shunt and 24 for the selective shunt. In two of the latter, a total shunt had to be performed for technical reasons. The fatality rates (six in the 24 total, and six in 22 selective [performed], and seven in 24 selective [randomized]), the frequency of shunt occlusion (two in each group), and of recurrent gastronintestinal bleeding (three in each group) were similar. Encephalopathy developed more often after a total shunt -- 10 of 24, or one per 58 patient-months -- than after selective (performed) -- one of 22, or one per 593 patient-months (P less than 0.005). Total shunts consistently diverted the hepatopetal mesenteric-portal flow from the liver. Deterioration of hepatic function (maximum rate of urea synthesis) was greater after total than selective shunt (P less than 0.05)."}
{"id": "PMID:1086429", "title": "Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases.", "content": "Extractable nuclear antigen contains ribo-nuclease-sensitive (ribonucleoprotein) and ribonuclease-resistant (Sm) components. To determine the diagnostic usefulness of antibodies to these antigens, a multicenter study was undertaken in which serums were analyzed for these antibodies and the findings compared with clinical and other laboratory characteristics of the patients. Of 100 patients with hemagglutinating antibodies to ribonuclease-sensitive extractable nuclear antigen, and only the same antibodies by immunodiffusion, 74 per cent had typical features of mixed connective-tissue disease; 12 features of systemic lupus erythematosus, eight those of scleroderma and six an undifferentiated mild connective-tissue disease. Of 27 patients with hemagglutinating antibodies to ribonuclease-resistant extractable nuclear antigen (and Sm antibodies by immunodiffusion), 85 per cent had typical systemic lupus. Thus, antibodies to nuclear ribonucleoprotein and Sm are of diagnostic use; if the serum contains only ribonucleoprotein antibody in high titer, it is likely that the patient has mixed connective-tissue disease.", "contents": "Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases. Extractable nuclear antigen contains ribo-nuclease-sensitive (ribonucleoprotein) and ribonuclease-resistant (Sm) components. To determine the diagnostic usefulness of antibodies to these antigens, a multicenter study was undertaken in which serums were analyzed for these antibodies and the findings compared with clinical and other laboratory characteristics of the patients. Of 100 patients with hemagglutinating antibodies to ribonuclease-sensitive extractable nuclear antigen, and only the same antibodies by immunodiffusion, 74 per cent had typical features of mixed connective-tissue disease; 12 features of systemic lupus erythematosus, eight those of scleroderma and six an undifferentiated mild connective-tissue disease. Of 27 patients with hemagglutinating antibodies to ribonuclease-resistant extractable nuclear antigen (and Sm antibodies by immunodiffusion), 85 per cent had typical systemic lupus. Thus, antibodies to nuclear ribonucleoprotein and Sm are of diagnostic use; if the serum contains only ribonucleoprotein antibody in high titer, it is likely that the patient has mixed connective-tissue disease."}
{"id": "PMID:1086438", "title": "[Effect of vestibular, somatic and autonomic afferent impulsation on the activity of individual neurons in the nuclei of the cat vestibular complex].", "content": "Responses of single vestibular neurons were studied in cats under adequate and electric stimulation of different afferent sources (labyrinths, mixed nerves, n. vagus, n. depressor, limb movements). Different types of responses to the employed stimulation as well as evoked activity as bursts followed by depression of background firing are described. The activity of vestibular neurons is shown to depend on extralabyrinthal stimulation. The role of muscle-joint receptors is distinguished from natural stimulation of the vestibular apparatus during the head tilting. Somatotopic organization of vestibular nuclei and convergence of different afferental impulses to the neurons forming the vestibulospinal tract are discussed.", "contents": "[Effect of vestibular, somatic and autonomic afferent impulsation on the activity of individual neurons in the nuclei of the cat vestibular complex]. Responses of single vestibular neurons were studied in cats under adequate and electric stimulation of different afferent sources (labyrinths, mixed nerves, n. vagus, n. depressor, limb movements). Different types of responses to the employed stimulation as well as evoked activity as bursts followed by depression of background firing are described. The activity of vestibular neurons is shown to depend on extralabyrinthal stimulation. The role of muscle-joint receptors is distinguished from natural stimulation of the vestibular apparatus during the head tilting. Somatotopic organization of vestibular nuclei and convergence of different afferental impulses to the neurons forming the vestibulospinal tract are discussed."}
{"id": "PMID:1086439", "title": "[A new valve controlled system for ventricular drainage. (Technical description and clinical results)].", "content": "A new valve controlled system for continuous ventricular drainage has been described. The clinical experiences (114 patients) are very satisfactory, even after a drainage duration of 15 days. The main advantages of the method are the resistance of all connexions against traction, the fixed position of the ventricular catheter, the variable opening pressure of the valve and the insensitivity to air or gas bubbles, at last the protection of the valve against blood and protein clots by use of filters. We have used the system for the continuous monitoring of the ICP and for diagnostic purposes (ventriculography, examination of cerebrospinal fluid) with good results.", "contents": "[A new valve controlled system for ventricular drainage. (Technical description and clinical results)]. A new valve controlled system for continuous ventricular drainage has been described. The clinical experiences (114 patients) are very satisfactory, even after a drainage duration of 15 days. The main advantages of the method are the resistance of all connexions against traction, the fixed position of the ventricular catheter, the variable opening pressure of the valve and the insensitivity to air or gas bubbles, at last the protection of the valve against blood and protein clots by use of filters. We have used the system for the continuous monitoring of the ICP and for diagnostic purposes (ventriculography, examination of cerebrospinal fluid) with good results."}
{"id": "PMID:1086443", "title": "Radiodiagnosis of intracranial pearly tumours with particular reference to the value of computer tomography.", "content": "The radiological appearances of 22 histologically proven epidermoid and dermoid tumours occurring in four sites are presented - (1) the petrous apex and cerebellopontine angle, (2) the suprasellar region, (3) the cerebral hemispheres and (4) the cerebellum and 4th ventricle. Pathognomonic radiological changes are only uncommonly demonstrated by conventional neuroradiological techniques. Computer assisted tomography employing an EMI scanner has demonstrated diagnostic appearances. The availability of detailed density changes occurring within the tumours together with additional intracranial information makes computer tomography the investigation of choice.", "contents": "Radiodiagnosis of intracranial pearly tumours with particular reference to the value of computer tomography. The radiological appearances of 22 histologically proven epidermoid and dermoid tumours occurring in four sites are presented - (1) the petrous apex and cerebellopontine angle, (2) the suprasellar region, (3) the cerebral hemispheres and (4) the cerebellum and 4th ventricle. Pathognomonic radiological changes are only uncommonly demonstrated by conventional neuroradiological techniques. Computer assisted tomography employing an EMI scanner has demonstrated diagnostic appearances. The availability of detailed density changes occurring within the tumours together with additional intracranial information makes computer tomography the investigation of choice."}
{"id": "PMID:1086440", "title": "[Spontaneous ventriculostomy developing in non-communicating hydrocephalus of unestablished etiology].", "content": "The authors report a case of exceptionally rarely occurring spontaneous ventriculostomy in the region of epiphyseal recess in a patient with increasing non-communicating hydrocephalus. This case is the fourth one diagnosed intravitally in a group of 22 cases published as yet. Positive ventriculography was the method establishing the diagnosis of this condition during life. Restoration of communication in intracranial cerebrospinal pathways with bypassing of the blockade by development of spontaneous ventriculostomy is regarded as a trial of self-treatment of the organism. The observations demonstrated that this bypassing in a group of 22 cases published as yet. Positive ventriculography was the method establishing the diagnosis of this condition during glife. Restoration of comis, in such cases, creation of ventriculo-atrial communication for reduction of intracranial pressure.", "contents": "[Spontaneous ventriculostomy developing in non-communicating hydrocephalus of unestablished etiology]. The authors report a case of exceptionally rarely occurring spontaneous ventriculostomy in the region of epiphyseal recess in a patient with increasing non-communicating hydrocephalus. This case is the fourth one diagnosed intravitally in a group of 22 cases published as yet. Positive ventriculography was the method establishing the diagnosis of this condition during life. Restoration of communication in intracranial cerebrospinal pathways with bypassing of the blockade by development of spontaneous ventriculostomy is regarded as a trial of self-treatment of the organism. The observations demonstrated that this bypassing in a group of 22 cases published as yet. Positive ventriculography was the method establishing the diagnosis of this condition during glife. Restoration of comis, in such cases, creation of ventriculo-atrial communication for reduction of intracranial pressure."}
{"id": "PMID:1086444", "title": "Ventriculography with Amipaque.", "content": "Greater diagnostic accuracy of lesions of the third and fourth ventricles and aqueduct has resulted from the use of positive contrast ventriculography with the watersoluble medium Amipaque. The examination is performed more easily than with gas ventriculography and has caused no distress or complication in 47 patients.", "contents": "Ventriculography with Amipaque. Greater diagnostic accuracy of lesions of the third and fourth ventricles and aqueduct has resulted from the use of positive contrast ventriculography with the watersoluble medium Amipaque. The examination is performed more easily than with gas ventriculography and has caused no distress or complication in 47 patients."}
{"id": "PMID:1086450", "title": "Plasma alpha-1-antitrypsin levels in early and late carcinoma of the cervix.", "content": "Previous studies have demonstrated a rise in serum alpha-1-antitrypsin at advanced stages of malignancy. These, together with results of animal experiments with another anti-proteinase, aprotinin, have prompted studies of the plasma alpha-1-antitrypsin in the early stages of malignancy, using cervical carcinoma as a model. Measurements were made in three groups of individuals; negative cervical-smear women volunteers; hospitalised women with a positive result from the cervical-smear test; hospitalised women with a clinical cervical carcinoma. Elevated plasma alpha-1-antitrypsin levels were found in the \"smear-positive\" and \"cancer\" groups. Possible explanations for this increase are discussed and it is concluded that it occurs in direct response to the early stages of development of the tumor. Once a frank carcinoma has developed, maximal response has been attained, regardless of the degree of invasion or metastasis.", "contents": "Plasma alpha-1-antitrypsin levels in early and late carcinoma of the cervix. Previous studies have demonstrated a rise in serum alpha-1-antitrypsin at advanced stages of malignancy. These, together with results of animal experiments with another anti-proteinase, aprotinin, have prompted studies of the plasma alpha-1-antitrypsin in the early stages of malignancy, using cervical carcinoma as a model. Measurements were made in three groups of individuals; negative cervical-smear women volunteers; hospitalised women with a positive result from the cervical-smear test; hospitalised women with a clinical cervical carcinoma. Elevated plasma alpha-1-antitrypsin levels were found in the \"smear-positive\" and \"cancer\" groups. Possible explanations for this increase are discussed and it is concluded that it occurs in direct response to the early stages of development of the tumor. Once a frank carcinoma has developed, maximal response has been attained, regardless of the degree of invasion or metastasis."}
{"id": "PMID:1086459", "title": "Inactin concentration in plasma of rats during anaesthesia and the effect of this concentration on short circuit current of isolated frog skin.", "content": "Inactin, given to rats for anaesthesia at a dose of 100 mg per kg body weight results in an arterial plasma concentration of 10.6 +/- 1.14 mg%, measured 40 min after the onset of anaesthesia. When similar concentrations of Inactin or Amytal were applied to frog skins the decrease in the in vitro short circuit current is dose dependent. With Inactin, the inhibition was, at least in part, reversible and the electrical conductance was not affected. Amytal however was found to depress SCC and electrical conductance irreversibly. The data are discussed in regard to active renal transport processes.", "contents": "Inactin concentration in plasma of rats during anaesthesia and the effect of this concentration on short circuit current of isolated frog skin. Inactin, given to rats for anaesthesia at a dose of 100 mg per kg body weight results in an arterial plasma concentration of 10.6 +/- 1.14 mg%, measured 40 min after the onset of anaesthesia. When similar concentrations of Inactin or Amytal were applied to frog skins the decrease in the in vitro short circuit current is dose dependent. With Inactin, the inhibition was, at least in part, reversible and the electrical conductance was not affected. Amytal however was found to depress SCC and electrical conductance irreversibly. The data are discussed in regard to active renal transport processes."}
{"id": "PMID:1086460", "title": "The intracellular electrical potential profile of the frog skin epithelium.", "content": "The potential profile of the frog skin epithelium incubated in Cl-Ringer was reinvestigated with improved technique. Under open circuit conditions (PDtr up to 120 mV) the potential profile was demonstrated to be trough like in contrast to the stair-step like potential profile reported by previous investigators which has probably been recorded after injuring of the punctured cells. In 67 successful impalements the potential difference across the basolateral membranes was 108 +/- 2 mV. The potential across the outer, epithelial-facing membrane was inversely related to the transepithelial PD, but was found to be negative (with respect to the epithelial bathing solution) in all punctures. Electrogenic sodium transport might be responsible for part of the potential difference across the basolateral membranes which correlatee directly with the short circuited state was -73 +/- i mV and decreased with increasing short cirucit current. It is suggested that these changes result from variations of the outer membrane sodium conductance.", "contents": "The intracellular electrical potential profile of the frog skin epithelium. The potential profile of the frog skin epithelium incubated in Cl-Ringer was reinvestigated with improved technique. Under open circuit conditions (PDtr up to 120 mV) the potential profile was demonstrated to be trough like in contrast to the stair-step like potential profile reported by previous investigators which has probably been recorded after injuring of the punctured cells. In 67 successful impalements the potential difference across the basolateral membranes was 108 +/- 2 mV. The potential across the outer, epithelial-facing membrane was inversely related to the transepithelial PD, but was found to be negative (with respect to the epithelial bathing solution) in all punctures. Electrogenic sodium transport might be responsible for part of the potential difference across the basolateral membranes which correlatee directly with the short circuited state was -73 +/- i mV and decreased with increasing short cirucit current. It is suggested that these changes result from variations of the outer membrane sodium conductance."}
{"id": "PMID:1086461", "title": "Ionic mechanism of inotropic effect of prostaglandin E1 on frog atrial muscle.", "content": "Prostaglandin E1 increases the amplitude and duration of cardiac action potential and exerts a positive inotropic effect on the frog heart. In voltage-clamp experiments, prostaglandin E1 increases both, the slow inward current and the delayed outward as well as the two components of the heart contraction. These results imply that calcium influx and the release of intracellular calcium are involved in the inotropic action of prostaglandin E1.", "contents": "Ionic mechanism of inotropic effect of prostaglandin E1 on frog atrial muscle. Prostaglandin E1 increases the amplitude and duration of cardiac action potential and exerts a positive inotropic effect on the frog heart. In voltage-clamp experiments, prostaglandin E1 increases both, the slow inward current and the delayed outward as well as the two components of the heart contraction. These results imply that calcium influx and the release of intracellular calcium are involved in the inotropic action of prostaglandin E1."}
{"id": "PMID:1086471", "title": "[Life-threathening tracheal obstruction following intubation in a case of Willebrand-J\u00fcrgens disease].", "content": "A case of severe dyspnoea is reported which occurred in a 21-years-old woman with Willebrand-J\u00fcrgens disease four days after intubation during a dental operation. A 34 Charri\u00e8re catheter was introduced into the glottis after a 22 Charri\u00e8re catheter had failed to pass the obstruction. The patient was then artificially ventilated by means of a Spiromat and was subsequently tracheotomized after her cardio-pulmonary condition had become stabilized. The cause of the dyspnoea was a fibrin clot of 5-6 cm length. Three weeks after the tracheotomy and one week after extubation the patient was discharged from hospital in a satisfactory condition. The factors responsible for the development of the fibrin clot are discussed. If there is persistent stridor and aphonia after extubation a laryngologist should be consulted.", "contents": "[Life-threathening tracheal obstruction following intubation in a case of Willebrand-J\u00fcrgens disease]. A case of severe dyspnoea is reported which occurred in a 21-years-old woman with Willebrand-J\u00fcrgens disease four days after intubation during a dental operation. A 34 Charri\u00e8re catheter was introduced into the glottis after a 22 Charri\u00e8re catheter had failed to pass the obstruction. The patient was then artificially ventilated by means of a Spiromat and was subsequently tracheotomized after her cardio-pulmonary condition had become stabilized. The cause of the dyspnoea was a fibrin clot of 5-6 cm length. Three weeks after the tracheotomy and one week after extubation the patient was discharged from hospital in a satisfactory condition. The factors responsible for the development of the fibrin clot are discussed. If there is persistent stridor and aphonia after extubation a laryngologist should be consulted."}
{"id": "PMID:1086475", "title": "Evidence for the lack of feedback regulation of gene activity and for the absence of subunit exchange between lactate dehydrogenase tetramers in vivo.", "content": "Frog and rat lactate dehydrogenase (LDH, L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) isozymes, highly purfied by affinity chromatography, were injected into newly fertilized from (Rana pipiens) eggs to study the effect of the injected isozymes on the expression of the LDH genes of the developing frog embryo. From these experiments three conclusions can be drawn: (i) homologous and heterologous LDH, even when present in more than twice the normal amount of LDH, does not play any role in the switching on or off of the frog LDH genes or in regulating the level of gene activity. No evidence was found in support of any feedback regulation of LDH synthesis by the LDH molecule itself. (ii) Injected rat LDH is very stable in the frog embryo, and the contrary to what can be demonstrated in vitro, all the isozymes show the same stability. No evidence was found for selective degradation of any of the injected five isozymes. (iii) The fact that the injected isozymes disappear 12 days after the injection without forming hybrid molecules between themselves or with the stored or newly synthesized frog LDH is evidence in favor of the hypothesis that the tetramer is the degradative unit of LDH, not the monomer as others have postulated. The LDH tetramers do not dissociate the recombine under the physiological conditions present in frog cells.", "contents": "Evidence for the lack of feedback regulation of gene activity and for the absence of subunit exchange between lactate dehydrogenase tetramers in vivo. Frog and rat lactate dehydrogenase (LDH, L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) isozymes, highly purfied by affinity chromatography, were injected into newly fertilized from (Rana pipiens) eggs to study the effect of the injected isozymes on the expression of the LDH genes of the developing frog embryo. From these experiments three conclusions can be drawn: (i) homologous and heterologous LDH, even when present in more than twice the normal amount of LDH, does not play any role in the switching on or off of the frog LDH genes or in regulating the level of gene activity. No evidence was found in support of any feedback regulation of LDH synthesis by the LDH molecule itself. (ii) Injected rat LDH is very stable in the frog embryo, and the contrary to what can be demonstrated in vitro, all the isozymes show the same stability. No evidence was found for selective degradation of any of the injected five isozymes. (iii) The fact that the injected isozymes disappear 12 days after the injection without forming hybrid molecules between themselves or with the stored or newly synthesized frog LDH is evidence in favor of the hypothesis that the tetramer is the degradative unit of LDH, not the monomer as others have postulated. The LDH tetramers do not dissociate the recombine under the physiological conditions present in frog cells."}
{"id": "PMID:1086476", "title": "Cells that express viral antigens but lack H-2 determinants are not lysed by immune thymus-derived lymphocytes but are lysed by other antiviral immune attack mechanisms.", "content": "Murine F9 teratoma cells do not express major transplantation antigens detectable by either serologic or alloreactive assays of thymus-dependent lymphocytes (T cells). Such cells can be infected with lymphocytic choriomeningitis virus or vaccinia virus, do express viral antigens on the cell surface, and can release infectious virus in amounts equivalent to those of other H-2 bearing murine cell lines. Immunologic injury of virus-infected F9 cells occurs after the addition of specific antiviral antibody and complement or of specific antiviral antibody and unsensitized lymphoid cells (antibody-mediated cell-dependent killing). In contrast, injury does not follow the addition of immune cytotoxic T cells. These results indicate that possession of H-2 antigens is not a requirement for a cell's infection by or production of virus. Further, expression of viral antigens on the cell's surface, although adequate for antibody-mediated immune injury, is by itself insufficient for direct T cell-mediated lysis. The major transplantation antigens thus probably represent the cell surface structures that are crucial for T-mediated cell wall damage that results in chromium release.", "contents": "Cells that express viral antigens but lack H-2 determinants are not lysed by immune thymus-derived lymphocytes but are lysed by other antiviral immune attack mechanisms. Murine F9 teratoma cells do not express major transplantation antigens detectable by either serologic or alloreactive assays of thymus-dependent lymphocytes (T cells). Such cells can be infected with lymphocytic choriomeningitis virus or vaccinia virus, do express viral antigens on the cell surface, and can release infectious virus in amounts equivalent to those of other H-2 bearing murine cell lines. Immunologic injury of virus-infected F9 cells occurs after the addition of specific antiviral antibody and complement or of specific antiviral antibody and unsensitized lymphoid cells (antibody-mediated cell-dependent killing). In contrast, injury does not follow the addition of immune cytotoxic T cells. These results indicate that possession of H-2 antigens is not a requirement for a cell's infection by or production of virus. Further, expression of viral antigens on the cell's surface, although adequate for antibody-mediated immune injury, is by itself insufficient for direct T cell-mediated lysis. The major transplantation antigens thus probably represent the cell surface structures that are crucial for T-mediated cell wall damage that results in chromium release."}
{"id": "PMID:1086478", "title": "Studies on insulin action. V. Structural requirements of primers for subsequent accumulation of D-glucose at 0 degrees C in insulinized frog muscles.", "content": "Systematic analysis was made of structural requirements for possible priming action of D-glucose and 40 related compounds in the presence of insulin to enhance subsequent labeled D-glucose uptake at 0 degrees C. It was concluded that a major portion if not all of the H-bonding groups on the D-glucose molecules are involved although their relative importance varies. Free OH and H on C-1, and upward orientation of OH and downward orientation of H on C-3, are the most important. Orientation of OH, H, and CH2OH on C-2, C-4, and C-5 are also important, but their perturbations are not nearly as detrimental to the priming action as those on C-1 and C-3.", "contents": "Studies on insulin action. V. Structural requirements of primers for subsequent accumulation of D-glucose at 0 degrees C in insulinized frog muscles. Systematic analysis was made of structural requirements for possible priming action of D-glucose and 40 related compounds in the presence of insulin to enhance subsequent labeled D-glucose uptake at 0 degrees C. It was concluded that a major portion if not all of the H-bonding groups on the D-glucose molecules are involved although their relative importance varies. Free OH and H on C-1, and upward orientation of OH and downward orientation of H on C-3, are the most important. Orientation of OH, H, and CH2OH on C-2, C-4, and C-5 are also important, but their perturbations are not nearly as detrimental to the priming action as those on C-1 and C-3."}
{"id": "PMID:1086501", "title": "[Current place of hemorrhagic rectocolitis in intestinal pathology].", "content": "Rectocolitis remains, at the present time, in spite of the large amount of work carried out, a condition of which the cause and the physiopathological mechanism are unknown: none of the theories proposed has been confirmed by the facts; none has made it possible to propose an effective therapeutic regimen. The diagnosis of haemorrhagic rectocolitis rests solely on an assembly of clinical, radiological, and anatomological findings, together with findings on progress of the disease; none of these findings taken separately being pathognomonic. Because of this it is essential in cases of inflammatory colic disorders to analyse critically these different elements before affirming the diagnosis that is often arrived at too easily. Different affections, even apart from Crohn's disease (parasitic, microbial, and iatrogenic affections, etc) may, in fact, give rise to radiological and clinical pictures close to those of haemorrhagic rectocolitis.", "contents": "[Current place of hemorrhagic rectocolitis in intestinal pathology]. Rectocolitis remains, at the present time, in spite of the large amount of work carried out, a condition of which the cause and the physiopathological mechanism are unknown: none of the theories proposed has been confirmed by the facts; none has made it possible to propose an effective therapeutic regimen. The diagnosis of haemorrhagic rectocolitis rests solely on an assembly of clinical, radiological, and anatomological findings, together with findings on progress of the disease; none of these findings taken separately being pathognomonic. Because of this it is essential in cases of inflammatory colic disorders to analyse critically these different elements before affirming the diagnosis that is often arrived at too easily. Different affections, even apart from Crohn's disease (parasitic, microbial, and iatrogenic affections, etc) may, in fact, give rise to radiological and clinical pictures close to those of haemorrhagic rectocolitis."}
{"id": "PMID:1086503", "title": "[Rheumatic manifestations of Crohn's disease. A national survey].", "content": "The authors analyse the results of a national survey, by questionnaire, in which 44 rheumatologists participated: the 102 cases of Crohn's disease discovered are divided as follow - 47 cases of peripheral rheumatism, and 55 cases of axial rheumatism, spondylarthritis and sacro-ileitis. The cases of peripheral rheumatism were closely related to the enteritis: appearing after the first digestive symptoms, they follow closely the development of, and recede with intestinal resection of the lesion; mono, oligo, or polyarticular, they develop in brief episodes, in an immune context and are only one symptom among the others of Crohn's disease. In contrast, preceding or following the first digestive symptoms, pelvispondylitis (34 cases) developed quite independently of the enteritis and was modified neither by medical treatment nor surgical treatment; these cases were characterized by equal distribution between the two sexes, inconstancy of syndesmosis, and frequent peripheral involvement. As regards the cases of sacro-ileitis, whether discovered by chance or because of rachialgia and arthralgia, these appear to be similar to the cases of pelvispondylitis and their appearance is probably conditioned by a polygenic transmission factor different from the HL-A B 27 marker.", "contents": "[Rheumatic manifestations of Crohn's disease. A national survey]. The authors analyse the results of a national survey, by questionnaire, in which 44 rheumatologists participated: the 102 cases of Crohn's disease discovered are divided as follow - 47 cases of peripheral rheumatism, and 55 cases of axial rheumatism, spondylarthritis and sacro-ileitis. The cases of peripheral rheumatism were closely related to the enteritis: appearing after the first digestive symptoms, they follow closely the development of, and recede with intestinal resection of the lesion; mono, oligo, or polyarticular, they develop in brief episodes, in an immune context and are only one symptom among the others of Crohn's disease. In contrast, preceding or following the first digestive symptoms, pelvispondylitis (34 cases) developed quite independently of the enteritis and was modified neither by medical treatment nor surgical treatment; these cases were characterized by equal distribution between the two sexes, inconstancy of syndesmosis, and frequent peripheral involvement. As regards the cases of sacro-ileitis, whether discovered by chance or because of rachialgia and arthralgia, these appear to be similar to the cases of pelvispondylitis and their appearance is probably conditioned by a polygenic transmission factor different from the HL-A B 27 marker."}
{"id": "PMID:1086504", "title": "[Dysenteric rheumatism. What remains in 1976?].", "content": "Joint manifestations that occur during or after an episode of dysentery, grouped under the name of dysenteric rheumatism, have been known since antiquity, and have been mainly found in military circles, in camps, or during military campaigns. After reviewing the principal epidemics of rheumatism complicated by joint manifestations, the authors show that the only different feature about what is called dysenteric rheumatism is the particular character of the joint manifestations which may be isolated or associated with other manifestations, notably ocular or urethral, forming then what is called the Fiessinger-Leroy-Reiter syndrome. The role of the dysentery bacillus in the genesis of the joint lesions is not clear. Although it may been suspected on the basis of epidemiological or bacteriological arguments in some cases, it has never been formally demonstrated and is often non-existent. The term dysenteric rheumatism is too restrictive and should henceforth be abandoned.", "contents": "[Dysenteric rheumatism. What remains in 1976?]. Joint manifestations that occur during or after an episode of dysentery, grouped under the name of dysenteric rheumatism, have been known since antiquity, and have been mainly found in military circles, in camps, or during military campaigns. After reviewing the principal epidemics of rheumatism complicated by joint manifestations, the authors show that the only different feature about what is called dysenteric rheumatism is the particular character of the joint manifestations which may be isolated or associated with other manifestations, notably ocular or urethral, forming then what is called the Fiessinger-Leroy-Reiter syndrome. The role of the dysentery bacillus in the genesis of the joint lesions is not clear. Although it may been suspected on the basis of epidemiological or bacteriological arguments in some cases, it has never been formally demonstrated and is often non-existent. The term dysenteric rheumatism is too restrictive and should henceforth be abandoned."}
{"id": "PMID:1086505", "title": "Passage of barium meal through esophagus in patients with balloon tamponade of esophageal varices.", "content": "Four patients in different states of consciousness in treatment with Sengstaken-Blakemore tube were given a barium meal. In every case the contrast medium passed the balloons and was cleared from the upper esophagus in less than four minutes, and none of it was aspirated to the bronchi.", "contents": "Passage of barium meal through esophagus in patients with balloon tamponade of esophageal varices. Four patients in different states of consciousness in treatment with Sengstaken-Blakemore tube were given a barium meal. In every case the contrast medium passed the balloons and was cleared from the upper esophagus in less than four minutes, and none of it was aspirated to the bronchi."}
{"id": "PMID:1086506", "title": "Antibody-dependent lymphocyte-mediated cytotoxicity. The nature of the effector cell and characterization of its fc receptor.", "content": "The effector cell in abtibody-dependent lymphocyte-mediated cytotoxicity (ADLMC) have been studied. The cells do not adhere to glass and bear Fc receptors independent of surface Ig and complement receptors. Whereas monocytes have IgG1 and Ig G3 Fc receptors, these Fc receptor lymphocytes appear to have receptors for aggregates of all four IgG subclasses.", "contents": "Antibody-dependent lymphocyte-mediated cytotoxicity. The nature of the effector cell and characterization of its fc receptor. The effector cell in abtibody-dependent lymphocyte-mediated cytotoxicity (ADLMC) have been studied. The cells do not adhere to glass and bear Fc receptors independent of surface Ig and complement receptors. Whereas monocytes have IgG1 and Ig G3 Fc receptors, these Fc receptor lymphocytes appear to have receptors for aggregates of all four IgG subclasses."}
{"id": "PMID:1086507", "title": "Prolonged treatment with tolfenamic acid in inflammatory rheumatic diseases.", "content": "The aim of this study was in the first place to elucidate the tolerance of rheumatic patients to prolonged treatment with tolfenamic acid. 91 patients took part in the trial, most of them suffering from rheumatoid arthritis. The daily dose of tolfenamic acid was 600 mg and the trial lasted 6 months. The side-effects were usually slight: diarrhea occurred in 8, other gastrointestinal disturbances in 11, dysuria in 8 and other side-effects in 3 cases. Six patients broke off their treatment because of side-effects ascribed to tolfenamic acid. However, laboratory tests failed to show any significant changes caused by this drug. One case of reversible thrombopenia was seen in a patient who had also received gold treatment. On the whole, the clinical effect was found to be good, and tolfenamic acid appeared to be well suited for long-term symptomatic treatment of rheumatics.", "contents": "Prolonged treatment with tolfenamic acid in inflammatory rheumatic diseases. The aim of this study was in the first place to elucidate the tolerance of rheumatic patients to prolonged treatment with tolfenamic acid. 91 patients took part in the trial, most of them suffering from rheumatoid arthritis. The daily dose of tolfenamic acid was 600 mg and the trial lasted 6 months. The side-effects were usually slight: diarrhea occurred in 8, other gastrointestinal disturbances in 11, dysuria in 8 and other side-effects in 3 cases. Six patients broke off their treatment because of side-effects ascribed to tolfenamic acid. However, laboratory tests failed to show any significant changes caused by this drug. One case of reversible thrombopenia was seen in a patient who had also received gold treatment. On the whole, the clinical effect was found to be good, and tolfenamic acid appeared to be well suited for long-term symptomatic treatment of rheumatics."}
{"id": "PMID:1086508", "title": "Haemophilus from the lower respiratory tract of patients with cystic fibrosis.", "content": "Fifty-six non-haemolytic Haemophilus strains were isolated during current bacteriological examinations from material from the lower respiratory tract of 39 patients with cystic fibrosis during a 6-month period. Except for six strains which belonged to Haemophilus parainfluenzae, all strains were identified as Haemophilus influenzae. Biotype I of H. influenzae was the predominating taxon (38%) and was especially related to patients with recurrent or prolonged colonization. Only two strains were capsulated. An unexpectedly high percentage of the strains produced lysine decarboxylase. The significance of this property in the pathogenesis of the respiratory tract colonization is discussed.", "contents": "Haemophilus from the lower respiratory tract of patients with cystic fibrosis. Fifty-six non-haemolytic Haemophilus strains were isolated during current bacteriological examinations from material from the lower respiratory tract of 39 patients with cystic fibrosis during a 6-month period. Except for six strains which belonged to Haemophilus parainfluenzae, all strains were identified as Haemophilus influenzae. Biotype I of H. influenzae was the predominating taxon (38%) and was especially related to patients with recurrent or prolonged colonization. Only two strains were capsulated. An unexpectedly high percentage of the strains produced lysine decarboxylase. The significance of this property in the pathogenesis of the respiratory tract colonization is discussed."}
{"id": "PMID:1086509", "title": "Lymphocyte mobilization in response to physical work in sarcoidosis. Influence on lymphocyte subpopulations, DNA synthesis and cytotoxicity.", "content": "Peripheral blood lymphocytes of nine patients with pulmonary sarcoidosis were collected before and after a standardized bicycle ergometer test. The lymphocytes were characterized by various cell surface markers as T or B lymphocytes, and functionally by mitogen- or antigen-induced DNA synthesis and by antibody dependent cell-mediated cytotoxicity. Physical work resulted in an increase of circulating lymphocytes. Proportionately more B lymphocytes were mobilized. The DNA synthesis of lymphocytes in response to mitogens and antigen was reduced, whereas the cytotoxicity was augmented. Compared with normals the mobilization of lymphocytes was less, and a deficiency of both T and B lymphocytes was revealed. The functional impairment of lymphocytes in the patient group persisted.", "contents": "Lymphocyte mobilization in response to physical work in sarcoidosis. Influence on lymphocyte subpopulations, DNA synthesis and cytotoxicity. Peripheral blood lymphocytes of nine patients with pulmonary sarcoidosis were collected before and after a standardized bicycle ergometer test. The lymphocytes were characterized by various cell surface markers as T or B lymphocytes, and functionally by mitogen- or antigen-induced DNA synthesis and by antibody dependent cell-mediated cytotoxicity. Physical work resulted in an increase of circulating lymphocytes. Proportionately more B lymphocytes were mobilized. The DNA synthesis of lymphocytes in response to mitogens and antigen was reduced, whereas the cytotoxicity was augmented. Compared with normals the mobilization of lymphocytes was less, and a deficiency of both T and B lymphocytes was revealed. The functional impairment of lymphocytes in the patient group persisted."}
{"id": "PMID:1086510", "title": "Photoreceptor shedding is initiated by light in the frog retina.", "content": "Frogs maintained on a diurnal light-dark cycle (14 hours light and 10 hours darkness) shed their rod photoreceptor outer segment tips shortly after the onset of light. Shedding is synchronous and occurs in about 25 percent of the rod photoreceptors each day. Prolonged exposure to total darkness decreases the amount of shedding, after which exposure to light results in a large burst of synchronous shedding. Thus in the frog retina, the synchronous shedding of rod outer segment tips is shown to be directly related to light stimulation.", "contents": "Photoreceptor shedding is initiated by light in the frog retina. Frogs maintained on a diurnal light-dark cycle (14 hours light and 10 hours darkness) shed their rod photoreceptor outer segment tips shortly after the onset of light. Shedding is synchronous and occurs in about 25 percent of the rod photoreceptors each day. Prolonged exposure to total darkness decreases the amount of shedding, after which exposure to light results in a large burst of synchronous shedding. Thus in the frog retina, the synchronous shedding of rod outer segment tips is shown to be directly related to light stimulation."}
{"id": "PMID:1086511", "title": "Modification of blood flow to the extremities by electrical stimulation of the nervous system.", "content": "Sixteen patients who had electrical stimulation applied to various portions of the nervous system were examined for increase in blood flow to the extremities. Clinical observations and a one-channel plethysmograph were used to measure arterial dilatation. Seven patients had transcutaneous stimulation applied over the cervical or thoracic spinal cord, peripheral nerves, or low lumbar region; eight had electrical stimulators implanted over the spinal cord in attempts to relieve intractable pain or some of the symptoms of multiple sclerosis; and one patient had electrical stimulators implanted over the C-6 dorsal roots for small artery disease of the upper extremities. Twelve of 13 patients who had electrical stimulation applied to the spinal cord or dorsal roots had significant arterial dilatation in one or more extremities. Electrical stimulation applied to the ulnar nerves did cause arterial dilatation. One patient did not show any change in the central arterial pressure curve during transcutaneous stimulation of the cervical spinal cord.", "contents": "Modification of blood flow to the extremities by electrical stimulation of the nervous system. Sixteen patients who had electrical stimulation applied to various portions of the nervous system were examined for increase in blood flow to the extremities. Clinical observations and a one-channel plethysmograph were used to measure arterial dilatation. Seven patients had transcutaneous stimulation applied over the cervical or thoracic spinal cord, peripheral nerves, or low lumbar region; eight had electrical stimulators implanted over the spinal cord in attempts to relieve intractable pain or some of the symptoms of multiple sclerosis; and one patient had electrical stimulators implanted over the C-6 dorsal roots for small artery disease of the upper extremities. Twelve of 13 patients who had electrical stimulation applied to the spinal cord or dorsal roots had significant arterial dilatation in one or more extremities. Electrical stimulation applied to the ulnar nerves did cause arterial dilatation. One patient did not show any change in the central arterial pressure curve during transcutaneous stimulation of the cervical spinal cord."}
{"id": "PMID:1086514", "title": "Cellular immunity to herpes simplex infection in humans.", "content": "A comparison of lymphocyte interferon production and transformation as in vitro parameters of cellular immunity to herpes simplex in humans in different types of mononuclear cell cultures is reported. Mononuclear cells were purified on Ficoll-Hypaque gradients and nylon fiber columns (purified T lymphocytes). Reactivity was then compared to that observed in autochthonous macrophage-lymphocyte cultures. The quantity and type (I or II) of interferon, as well as the magnitude of the transformation response, is affected by the type of cell culture used for assay. The kinetics of lymphocyte reactivity in doners studied within 12 weeks after disease were compared to those occurring at greater than 12 weeks after disease and in seronegative donors. The observed kinetics were variable according to the methods used to prepare mononuclear cells for culture.", "contents": "Cellular immunity to herpes simplex infection in humans. A comparison of lymphocyte interferon production and transformation as in vitro parameters of cellular immunity to herpes simplex in humans in different types of mononuclear cell cultures is reported. Mononuclear cells were purified on Ficoll-Hypaque gradients and nylon fiber columns (purified T lymphocytes). Reactivity was then compared to that observed in autochthonous macrophage-lymphocyte cultures. The quantity and type (I or II) of interferon, as well as the magnitude of the transformation response, is affected by the type of cell culture used for assay. The kinetics of lymphocyte reactivity in doners studied within 12 weeks after disease were compared to those occurring at greater than 12 weeks after disease and in seronegative donors. The observed kinetics were variable according to the methods used to prepare mononuclear cells for culture."}
{"id": "PMID:1086519", "title": "[Follow up study of patients operated on metastases of the lung and pleura (author's transl)].", "content": "From 1956 til 1974, 1851 patients have been operated on a malignancy of the lung. 29 of them had a solitary lung metastasis and 2 of them a metastasis of the pleura. Among those, the primary malignancies showed the following frequency: 7 malignancies of the uterus, 7 sarcomas, 4 carcinomas of the breast, 4 carcinomas of the large bowel, 4 malignant goiters, 3 hypernephromas, 1 seminoma and 1 malignancy of the pancreas. The mean survival time was 3.3 years. 25.8% of the patients survived 5 years. Patients with a primary malignancy of the uterus appear to do best. In case of repeated metastases we did not operate upon again.", "contents": "[Follow up study of patients operated on metastases of the lung and pleura (author's transl)]. From 1956 til 1974, 1851 patients have been operated on a malignancy of the lung. 29 of them had a solitary lung metastasis and 2 of them a metastasis of the pleura. Among those, the primary malignancies showed the following frequency: 7 malignancies of the uterus, 7 sarcomas, 4 carcinomas of the breast, 4 carcinomas of the large bowel, 4 malignant goiters, 3 hypernephromas, 1 seminoma and 1 malignancy of the pancreas. The mean survival time was 3.3 years. 25.8% of the patients survived 5 years. Patients with a primary malignancy of the uterus appear to do best. In case of repeated metastases we did not operate upon again."}
{"id": "PMID:1086520", "title": "[Resection for pulmonary metastases: results and prognosis (author's transl)].", "content": "From 1955 to 1975 in 25 patients lung surgery has been carried out because of intrapulmonary metastases of extrapulmonary malignant growth. 6 patients survived longer than 5 years. There was no relationship between survival time and length of the time interval between initial surgery (primary) and second operation (i.e. diagnosis of pulmonary lesion). On the other hand a definite relationship resulted for survival time and the size of the growth. In bad-risk patients surgery for metastatic lung cancer is not indicated, but it is very successful in selected cases.", "contents": "[Resection for pulmonary metastases: results and prognosis (author's transl)]. From 1955 to 1975 in 25 patients lung surgery has been carried out because of intrapulmonary metastases of extrapulmonary malignant growth. 6 patients survived longer than 5 years. There was no relationship between survival time and length of the time interval between initial surgery (primary) and second operation (i.e. diagnosis of pulmonary lesion). On the other hand a definite relationship resulted for survival time and the size of the growth. In bad-risk patients surgery for metastatic lung cancer is not indicated, but it is very successful in selected cases."}
{"id": "PMID:1086521", "title": "[Results after surgical treatment of solitary metastasis of the lung (author's transl)].", "content": "The surgery of solitary metastasis of the lung has special ranges of indication which refer to pathomorphology, cardiorespiratory and oncological programming. By systematical selection the own material of 45 cases shows a two year survival time of 33% and a five year of 11%. Last results with survival rate are similar to the treatment of the bronchus carcinoma of all stages and are further justifying this therapeutical consequence.", "contents": "[Results after surgical treatment of solitary metastasis of the lung (author's transl)]. The surgery of solitary metastasis of the lung has special ranges of indication which refer to pathomorphology, cardiorespiratory and oncological programming. By systematical selection the own material of 45 cases shows a two year survival time of 33% and a five year of 11%. Last results with survival rate are similar to the treatment of the bronchus carcinoma of all stages and are further justifying this therapeutical consequence."}
{"id": "PMID:1086522", "title": "[Early experiences with arterial heterografts (author's transl)].", "content": "Since April 1975 we used 50 bovine arterial heterografts for reconstructive surgery after chronic arterial occlusions or for the production of AV-shunts needed for chronic dialysis. In none of the cases incompatibility was seen. The early results (with a follow-up period of 6-10 months) after femoro-popliteal bypass were excellent, as far as a good peripheral run off was present. Several shunts (n = 7) containing 2-3 transplants, are still functioning 3-10 months after implantation. At present, we use bovine transplants in reconstructive vessel surgery when a thrombendarterectomy or the connection of venous grafts is impossible or when the operative procedure should be kept short in high-risk patients. To produce AV-shunts in patients with renal insufficiency we prefer this type of grafts when all conventional techniques were already used.", "contents": "[Early experiences with arterial heterografts (author's transl)]. Since April 1975 we used 50 bovine arterial heterografts for reconstructive surgery after chronic arterial occlusions or for the production of AV-shunts needed for chronic dialysis. In none of the cases incompatibility was seen. The early results (with a follow-up period of 6-10 months) after femoro-popliteal bypass were excellent, as far as a good peripheral run off was present. Several shunts (n = 7) containing 2-3 transplants, are still functioning 3-10 months after implantation. At present, we use bovine transplants in reconstructive vessel surgery when a thrombendarterectomy or the connection of venous grafts is impossible or when the operative procedure should be kept short in high-risk patients. To produce AV-shunts in patients with renal insufficiency we prefer this type of grafts when all conventional techniques were already used."}
{"id": "PMID:1086523", "title": "[Early and late complications due to direct and indirect aortic-isthmus-Plastic (author's transl)].", "content": "During the last 20 years 156 patients underwent a direct and indirect aortic-isthmus-plastic. Early complications occurred in 7.6%. 5 patients developed an aneurysm in the area of isthmus-plastic. The mortality rare was 4.5%. There was no evidence of recurrent coarctation in a group of children and in adult patients. The indirect aortic-isthmus-plastic first described by Vosschulte represents an elegant surgical method of functionally reestablishing the aortic lumen by retaining the posterior wall for further growth potential. This technique can be performed with a low mortality rate and no recurrent coarctation of the aorta.", "contents": "[Early and late complications due to direct and indirect aortic-isthmus-Plastic (author's transl)]. During the last 20 years 156 patients underwent a direct and indirect aortic-isthmus-plastic. Early complications occurred in 7.6%. 5 patients developed an aneurysm in the area of isthmus-plastic. The mortality rare was 4.5%. There was no evidence of recurrent coarctation in a group of children and in adult patients. The indirect aortic-isthmus-plastic first described by Vosschulte represents an elegant surgical method of functionally reestablishing the aortic lumen by retaining the posterior wall for further growth potential. This technique can be performed with a low mortality rate and no recurrent coarctation of the aorta."}
{"id": "PMID:1086524", "title": "[Open-heart surgery during deep hypothermia in infancy].", "content": "A total of 47 infants (2,7 to 9,6 kg in weight, 3 days to 21 months of age, 89% under one year) underwent early total correction of severe congenital heart defects in profound hypothermia of about 22degreesC and circulatory arrest (11 to 64 minutes). 8 patients were operated upon without extracorporeal circulation (ECC) (mortality 63%). 37 infants were additionally cooled and rewarmed with ECC (mortality 22%). The most common diagnosis were: Total atrioventricular canal (11), aortic stenosis with and without combined lesions (8), tetralogy of Fallot (7), ventricular septum defect (7) and transposition of the great arteries (4).", "contents": "[Open-heart surgery during deep hypothermia in infancy]. A total of 47 infants (2,7 to 9,6 kg in weight, 3 days to 21 months of age, 89% under one year) underwent early total correction of severe congenital heart defects in profound hypothermia of about 22degreesC and circulatory arrest (11 to 64 minutes). 8 patients were operated upon without extracorporeal circulation (ECC) (mortality 63%). 37 infants were additionally cooled and rewarmed with ECC (mortality 22%). The most common diagnosis were: Total atrioventricular canal (11), aortic stenosis with and without combined lesions (8), tetralogy of Fallot (7), ventricular septum defect (7) and transposition of the great arteries (4)."}
{"id": "PMID:1086525", "title": "[Surgical considerations on levo-atrial systemic vein (author's transl)].", "content": "The levo-atrial systemic vein is a special type of persistent left superior vena cava and is associated with other intracardiac lesions most often. Hemodynamically there is a right to left shunt. The operative procedure may consist in a ligature in relation to the anatomic situation. The intraatrial correction intends a drainage to the right atrium. Of 7 cases four times a intracardiac rerouting was achieved but two times a ligation was performed.", "contents": "[Surgical considerations on levo-atrial systemic vein (author's transl)]. The levo-atrial systemic vein is a special type of persistent left superior vena cava and is associated with other intracardiac lesions most often. Hemodynamically there is a right to left shunt. The operative procedure may consist in a ligature in relation to the anatomic situation. The intraatrial correction intends a drainage to the right atrium. Of 7 cases four times a intracardiac rerouting was achieved but two times a ligation was performed."}
{"id": "PMID:1086526", "title": "[Obstruction of the hepatic portion of the inferior vena cava: morphological characteristics and surgical treatment (author's transl)].", "content": "Operable obstructions of the hepatic portion of the inferior vena cava (IVC) are rare but probably diagnosed more frequently nowadays. Since 1969 we operated 2 patients, one of them twice. Concerning to these cases the problems of diagnosis, morphology and surgical treatment are discussed. In the patient, who were operated twice, we found the combination - which has not been reported in medical literature yet - of a membranous stenosis of the hepatic portion of the IVC and a large Eustachian valve obstructing the IVC.", "contents": "[Obstruction of the hepatic portion of the inferior vena cava: morphological characteristics and surgical treatment (author's transl)]. Operable obstructions of the hepatic portion of the inferior vena cava (IVC) are rare but probably diagnosed more frequently nowadays. Since 1969 we operated 2 patients, one of them twice. Concerning to these cases the problems of diagnosis, morphology and surgical treatment are discussed. In the patient, who were operated twice, we found the combination - which has not been reported in medical literature yet - of a membranous stenosis of the hepatic portion of the IVC and a large Eustachian valve obstructing the IVC."}
{"id": "PMID:1086527", "title": "[Some somatic and psychological predictors of psychopathological disorders after cardiac surgery (author's transl)].", "content": "Some new predictors of postoperative psychosis in open-heart surgery have been identified in a multifactorial study of somatic and psychological factors: Somatic predictors are lower body weight, higher blood levels of urea nitrogen and creatinine, preoperative use of tranquilizers, especially in patients with double valve replacement or congenital heart disease and preoperative cerebral embolism in mitral valve disease. Bypass-time has not proved to be a valid predictor. Psychological predictors are distress provoked by severity and duration of illness, repeated delay of surgery as well as the manner of psychological coping with the illness.", "contents": "[Some somatic and psychological predictors of psychopathological disorders after cardiac surgery (author's transl)]. Some new predictors of postoperative psychosis in open-heart surgery have been identified in a multifactorial study of somatic and psychological factors: Somatic predictors are lower body weight, higher blood levels of urea nitrogen and creatinine, preoperative use of tranquilizers, especially in patients with double valve replacement or congenital heart disease and preoperative cerebral embolism in mitral valve disease. Bypass-time has not proved to be a valid predictor. Psychological predictors are distress provoked by severity and duration of illness, repeated delay of surgery as well as the manner of psychological coping with the illness."}
{"id": "PMID:1086528", "title": "[Performance of the Lillehei-Kaster-prosthesis. A critical haemodynamic study (author's transl)].", "content": "11 patients were studied 6 to 12 months after aortic valve replacement with Lillehei-Kaster-prosthesis (LKP). We found pressure gradients (deltaP) at rest of 31 mmHg (7 to 52 mmHg), 37 mmHg (8 to 69 mmHg during exercise and 8 mmHg during ventricular pacing delta P was influenced by stroke volume and valve size, but not by the width of the aorta. The opening angle alpha of the disc was only 62degrees and depended on stroke volume and on the orientation of the value within the aorta. The blood passed the valve ring only through the larger part of the orifice, the opening area was only 50% of the given area. Thus in practice the LKP does not perform to manufacturers specification.", "contents": "[Performance of the Lillehei-Kaster-prosthesis. A critical haemodynamic study (author's transl)]. 11 patients were studied 6 to 12 months after aortic valve replacement with Lillehei-Kaster-prosthesis (LKP). We found pressure gradients (deltaP) at rest of 31 mmHg (7 to 52 mmHg), 37 mmHg (8 to 69 mmHg during exercise and 8 mmHg during ventricular pacing delta P was influenced by stroke volume and valve size, but not by the width of the aorta. The opening angle alpha of the disc was only 62degrees and depended on stroke volume and on the orientation of the value within the aorta. The blood passed the valve ring only through the larger part of the orifice, the opening area was only 50% of the given area. Thus in practice the LKP does not perform to manufacturers specification."}
{"id": "PMID:1086529", "title": "[The Lillehei-Kaster valve prosthesis hemodynamic and mechanic features in vivo (author's transl)].", "content": "29 patients with Lillehei-Kaster vale prostheses, 22 in aortic position and 7 in mitral position, were examined. With small aortic valves (A 14 - A 16) mean gradients averaged 32 mm Hg, valve area varied between 1 and 1.2 cm2. Only size A 20 and larger showed satisfying gradient and valve areas of 1.9 cm2 and greater. The mitral gradients averaged between 10 and 5 mm Hg according to size and valve areas were about 1.6 cm2. The maximal measured opening angle of the pivoting disk averaged 64degrees in the aortic valves and 61degrees in the mitral valves; complete opening (80degrees) was not observed.", "contents": "[The Lillehei-Kaster valve prosthesis hemodynamic and mechanic features in vivo (author's transl)]. 29 patients with Lillehei-Kaster vale prostheses, 22 in aortic position and 7 in mitral position, were examined. With small aortic valves (A 14 - A 16) mean gradients averaged 32 mm Hg, valve area varied between 1 and 1.2 cm2. Only size A 20 and larger showed satisfying gradient and valve areas of 1.9 cm2 and greater. The mitral gradients averaged between 10 and 5 mm Hg according to size and valve areas were about 1.6 cm2. The maximal measured opening angle of the pivoting disk averaged 64degrees in the aortic valves and 61degrees in the mitral valves; complete opening (80degrees) was not observed."}
{"id": "PMID:1086530", "title": "[Clinical and hemodynamic evaluation on the functional performance of the Starr-Edwards low profile valve prosthese in mitral position (author's transl)].", "content": "205 patients with isolated mitral valve replacement using the Starr-Edwards low profile valve prostheses (model 6520 and 6550) were followed 3 to 67 months (mean of 30,4 months) after surgery. While these prostheses seems to reliable substitutes with regard to functional performance and to mechanical dysfunction, postoperative hemodynamic investigation revealed significant diastolic pressure gradients across the valve as well as reduction in functional mitral valve area relative to pulse and stroke volume. The incidence of thromboembolism was comparable to other artificial valves.", "contents": "[Clinical and hemodynamic evaluation on the functional performance of the Starr-Edwards low profile valve prosthese in mitral position (author's transl)]. 205 patients with isolated mitral valve replacement using the Starr-Edwards low profile valve prostheses (model 6520 and 6550) were followed 3 to 67 months (mean of 30,4 months) after surgery. While these prostheses seems to reliable substitutes with regard to functional performance and to mechanical dysfunction, postoperative hemodynamic investigation revealed significant diastolic pressure gradients across the valve as well as reduction in functional mitral valve area relative to pulse and stroke volume. The incidence of thromboembolism was comparable to other artificial valves."}
{"id": "PMID:1086531", "title": "[Hemodynamic studies after the implantation of Hancock-heart valves (author's transl)].", "content": "Velocity profile, pressure gradient across the valve and blood flow have been measured in 10 patients after the implantation of bioprostheses of the sizes 27 and 29 millimeters in aortic position. According to these hemodynamic measurements the use of bioprostheses for the aortic valve replacement is recommendable. The low flow velocities in the area of the muscle containing valve cusp did not effect a detectable stenosis intraoperatively under the conditions of rest. The hemodynamic data showed no differences between the Hancock-Zuhdi and the Edwards-Carpentier bioprostheses. Despite of good hemodynamic conditions and a low risk of thromboembolic complications after the aortic valve replacement with bioprostheses reservation is recommended to its clinical use until the long term mechanical stability (more than 6 years) of the biological heart valve prosthesis has been secured.", "contents": "[Hemodynamic studies after the implantation of Hancock-heart valves (author's transl)]. Velocity profile, pressure gradient across the valve and blood flow have been measured in 10 patients after the implantation of bioprostheses of the sizes 27 and 29 millimeters in aortic position. According to these hemodynamic measurements the use of bioprostheses for the aortic valve replacement is recommendable. The low flow velocities in the area of the muscle containing valve cusp did not effect a detectable stenosis intraoperatively under the conditions of rest. The hemodynamic data showed no differences between the Hancock-Zuhdi and the Edwards-Carpentier bioprostheses. Despite of good hemodynamic conditions and a low risk of thromboembolic complications after the aortic valve replacement with bioprostheses reservation is recommended to its clinical use until the long term mechanical stability (more than 6 years) of the biological heart valve prosthesis has been secured."}
{"id": "PMID:1086532", "title": "[Intraventricular conduit for complex forms of transposition of great vessels (author's transl)].", "content": "Complex forms of transposition of great vessels necessitate different approaches to correction. In a case of double outlet right ventricle with d-transposition and atrioventricular concordance and a subvalvular pulmonary stenosis an intraventricular conduit with a 16 mm Dacron vascular prosthesis was used. This was anastomosed in an acute angular manner with the muscular margins of the VSD and routed to the base of the aorta. Surgical and anatomic implications are discussed.", "contents": "[Intraventricular conduit for complex forms of transposition of great vessels (author's transl)]. Complex forms of transposition of great vessels necessitate different approaches to correction. In a case of double outlet right ventricle with d-transposition and atrioventricular concordance and a subvalvular pulmonary stenosis an intraventricular conduit with a 16 mm Dacron vascular prosthesis was used. This was anastomosed in an acute angular manner with the muscular margins of the VSD and routed to the base of the aorta. Surgical and anatomic implications are discussed."}
{"id": "PMID:1086533", "title": "[A new method for patch enlargement of hypoplastic aortic annulus. An experimental study (author's transl)].", "content": "The purpose of this communication is to propose a new surgical procedure to enlarge the hypoplastic aortic valve ring of more than 20 mm and to permit the replacement of the aortic valve with a suitable prosthesis. The aortic incision is extended about 20 mm across the fibrous origin of the mitral ring downwards into the aortic leaflet of the mitral valve. A fusiform Dacron patch was sutured to the defect in the aortic leaflet of the mitral valve with running suture lines. In acute experiments mitral valve abnormalities are excluded by hemodynamic data, angiocardiography and echocardiography.", "contents": "[A new method for patch enlargement of hypoplastic aortic annulus. An experimental study (author's transl)]. The purpose of this communication is to propose a new surgical procedure to enlarge the hypoplastic aortic valve ring of more than 20 mm and to permit the replacement of the aortic valve with a suitable prosthesis. The aortic incision is extended about 20 mm across the fibrous origin of the mitral ring downwards into the aortic leaflet of the mitral valve. A fusiform Dacron patch was sutured to the defect in the aortic leaflet of the mitral valve with running suture lines. In acute experiments mitral valve abnormalities are excluded by hemodynamic data, angiocardiography and echocardiography."}
{"id": "PMID:1086537", "title": "Presence of antigen-antibody complexes in antiallogeneic and antixenogeneic sera.", "content": "Antilymphocyte sera (ALS), either allogeneic (for example, Lewis anti-August rat serum) or xenogeneic (the classic ALS), may retain the antigen used for the immunization of the animal producing the serum in the form of antigen-antibody complexes, at least when the animal is bled within the first 2 weeks following the last immunizing injection. Evidence for this has been obtained in vivo and in vitro. In vivo experiments showed that injection of Lewis anti-August serum to a normal Lewis rat can induce a delayed active immunization against the August strain. In vitro studies proved the presence of August antigens in the anti-August sera, by precipitation of the antigen-antibody complexes with 6 M urea, removal of the antibody by filtration, and demonstration that the filtrate specifically inhibits complement-dependent cytotoxicity of anti-August antibodies. The interpretation of a number of previous works on so-called passive enhancement of grafts or on various effects of ALS may have to be reconsidered on the basis of these findings.", "contents": "Presence of antigen-antibody complexes in antiallogeneic and antixenogeneic sera. Antilymphocyte sera (ALS), either allogeneic (for example, Lewis anti-August rat serum) or xenogeneic (the classic ALS), may retain the antigen used for the immunization of the animal producing the serum in the form of antigen-antibody complexes, at least when the animal is bled within the first 2 weeks following the last immunizing injection. Evidence for this has been obtained in vivo and in vitro. In vivo experiments showed that injection of Lewis anti-August serum to a normal Lewis rat can induce a delayed active immunization against the August strain. In vitro studies proved the presence of August antigens in the anti-August sera, by precipitation of the antigen-antibody complexes with 6 M urea, removal of the antibody by filtration, and demonstration that the filtrate specifically inhibits complement-dependent cytotoxicity of anti-August antibodies. The interpretation of a number of previous works on so-called passive enhancement of grafts or on various effects of ALS may have to be reconsidered on the basis of these findings."}
{"id": "PMID:1086538", "title": "Transition from T cell protection to T cell enhancement during tumor growth in an allogeneic host.", "content": "The cell-mediated immune response of mice toward a lethal allogeneic tumor was investigated during tumor development. The activity of spleen cells from the tumor-bearing mice was studied by transferring them together with 3LL tumor cells into normal C3H/eb recipient mice. The activity depended upon the time interval between inoculation of the tumor and transfer. Spleen cells taken relatively early, 1 week after tumor inoculation, mediated protection against tumor growth. In contrast, spleen cells taken 4 weeks after tumor inoculation markedly enhanced tumor growth. The tumor-enhancing cells, like the tumor-protecting cells, appeared to be T lymphocytes. The enhancing activity could be transferred by extra cellular medium prepared by incubating the enhancing T cells. Protecting activity could not be transferred by cell-free medium prepared from the protecting T cells. Both activities were found to exist to a relatively slight degree in populations of spleen cells from normal mice. The transition from T cell protection to T cell enhancement might be a determining factor in the outcome of the host-tumor relationship.", "contents": "Transition from T cell protection to T cell enhancement during tumor growth in an allogeneic host. The cell-mediated immune response of mice toward a lethal allogeneic tumor was investigated during tumor development. The activity of spleen cells from the tumor-bearing mice was studied by transferring them together with 3LL tumor cells into normal C3H/eb recipient mice. The activity depended upon the time interval between inoculation of the tumor and transfer. Spleen cells taken relatively early, 1 week after tumor inoculation, mediated protection against tumor growth. In contrast, spleen cells taken 4 weeks after tumor inoculation markedly enhanced tumor growth. The tumor-enhancing cells, like the tumor-protecting cells, appeared to be T lymphocytes. The enhancing activity could be transferred by extra cellular medium prepared by incubating the enhancing T cells. Protecting activity could not be transferred by cell-free medium prepared from the protecting T cells. Both activities were found to exist to a relatively slight degree in populations of spleen cells from normal mice. The transition from T cell protection to T cell enhancement might be a determining factor in the outcome of the host-tumor relationship."}
{"id": "PMID:1086539", "title": "Antibody-dependent lymphocyte-mediated cytotoxicity (Ab-LMC), definition of the \"K cell\" in the rat.", "content": "The nature and membrane characteristics of the \"K cell\" of antibody-dependent lymphocyte-mediated cytotoxicity (Ab-LMC) were investigated in a widely used rat model of transplantation. Treatment of sensitized effector cell populations with anti-immunoglobulin and complement eliminated K cell cytotoxicity without diminishing the component of T cell-mediated injury. EA and EAC depletion experiments, although demonstrating no loss of K cell cytotoxicity after removal of complement receptor-bearing lymphocytes, produced a marked abrogation of cytotoxicity following the removal of the Fc receptor-bearing lymphocyte pool. Studies on phagocytic properties showed K cell activity to be shared by an adherent as well as a nonadherent cell population. Thus, the Fc receptor emerged as the only constant surface marker of the rat K cell in Ab-LMC.", "contents": "Antibody-dependent lymphocyte-mediated cytotoxicity (Ab-LMC), definition of the \"K cell\" in the rat. The nature and membrane characteristics of the \"K cell\" of antibody-dependent lymphocyte-mediated cytotoxicity (Ab-LMC) were investigated in a widely used rat model of transplantation. Treatment of sensitized effector cell populations with anti-immunoglobulin and complement eliminated K cell cytotoxicity without diminishing the component of T cell-mediated injury. EA and EAC depletion experiments, although demonstrating no loss of K cell cytotoxicity after removal of complement receptor-bearing lymphocytes, produced a marked abrogation of cytotoxicity following the removal of the Fc receptor-bearing lymphocyte pool. Studies on phagocytic properties showed K cell activity to be shared by an adherent as well as a nonadherent cell population. Thus, the Fc receptor emerged as the only constant surface marker of the rat K cell in Ab-LMC."}
{"id": "PMID:1086550", "title": "[Emergency endoscopy in acute upper gastrointestinal bleeding (author's transl)].", "content": "A report is given of 135 panendoscopic examinations in acute upper gastrointestinal bleeding. The importance of endoscopy is underlined by the high incidence of erosions and potential bleeding sources, since these lesions are not detected by radiological examination. Nor is it possible by means of X-rays to distinguish actual sources of haemorrhage from potential sources. The case history is only of limited value in pin-pointing the source of the haemorrhage. Endoscopy yields the best results in the diagnosis of the cause of gastrointestinal bleeding.", "contents": "[Emergency endoscopy in acute upper gastrointestinal bleeding (author's transl)]. A report is given of 135 panendoscopic examinations in acute upper gastrointestinal bleeding. The importance of endoscopy is underlined by the high incidence of erosions and potential bleeding sources, since these lesions are not detected by radiological examination. Nor is it possible by means of X-rays to distinguish actual sources of haemorrhage from potential sources. The case history is only of limited value in pin-pointing the source of the haemorrhage. Endoscopy yields the best results in the diagnosis of the cause of gastrointestinal bleeding."}
{"id": "PMID:1086551", "title": "[The laboratory diagnosis of von Willebrand's disease (author's transl)].", "content": "This investigation was undertaken in order to determine the most suitable methods for diagnosing von Willebrand's disease (v. W. d.), with particular reference to \"mild\" cases. 50 healthy persons, 21 patients with \"severe\" v.W.d. (verified according to all hitherto-established criteria) and 39 persons suffering from \"mild\" v.W.d. were examined. Even though - as far as the latter group is concerned - some of the characteristic laboratory findings were absent, the fact that these persons are related to the patients with \"severe\" v.W.d. made verification of the diagnosis nevertheless possible. In the group with \"mild\" v.W.d. 85% had decreased functional factor VIII activity and 82% showed reduced platelet adhesiveness; a prolonged bleeding time according to Borchgrevink was recorded in 72% of the cases and 64% had pathological values for the Ristocetin-induced platelet aggregation were pathological in less than 50% of the group. There was a close relation between the diagnostic significance and reproducibility of the various methods; in regard to the group of patients with \"mild\" v.W.d., the methods yielding the greatest number of pathological findings (functional factor VIII and platelet adhesiveness) were the ones with the lowest variation coefficients, i.e. 3.1 and 2.1%, respectively. The consideration of a time-dependent rise in the aggregation capability of washed platelets with Ristocetin was of decisive importance in the reproducibility of the rather sophisticated technique of determining the Ristocetin cofactor. The addition of EDTA largely inhabited this effect. Even in the group of patients with \"severe\" v.W.d., part of the findings obtained with screening tests of the intrinsic coagulation system remained within the normal range. There was no statistical correlation between the individual laboratory findings in the group of healthy persons. In the group with \"mild\" v.W.d., there was a significant correlation between platelet adhesiveness and Ristocetin-induced platelet aggregation on the one hand, and platelet adhesiveness and the Ristocetin cofactor on the other. In addition, a verified correlation was found to exist between Ristocetin-induced platelet aggregation and Ristocetin cofactor and between functional factor VIII and factor VIII-related antigen. In the group of \"severe\" v.W.d., only platelet adhesiveness and functional factor VIII were not significantly correlated, whilst all other correlations were of statistical significance. Further problems involved in the diagnosis of v.W.d., such as the variablility of laboratory findings, the existence of subgroups, as well as the difficulties involved in the statistical evaluation of individual cases suffering from v.W.d. are discussed.", "contents": "[The laboratory diagnosis of von Willebrand's disease (author's transl)]. This investigation was undertaken in order to determine the most suitable methods for diagnosing von Willebrand's disease (v. W. d.), with particular reference to \"mild\" cases. 50 healthy persons, 21 patients with \"severe\" v.W.d. (verified according to all hitherto-established criteria) and 39 persons suffering from \"mild\" v.W.d. were examined. Even though - as far as the latter group is concerned - some of the characteristic laboratory findings were absent, the fact that these persons are related to the patients with \"severe\" v.W.d. made verification of the diagnosis nevertheless possible. In the group with \"mild\" v.W.d. 85% had decreased functional factor VIII activity and 82% showed reduced platelet adhesiveness; a prolonged bleeding time according to Borchgrevink was recorded in 72% of the cases and 64% had pathological values for the Ristocetin-induced platelet aggregation were pathological in less than 50% of the group. There was a close relation between the diagnostic significance and reproducibility of the various methods; in regard to the group of patients with \"mild\" v.W.d., the methods yielding the greatest number of pathological findings (functional factor VIII and platelet adhesiveness) were the ones with the lowest variation coefficients, i.e. 3.1 and 2.1%, respectively. The consideration of a time-dependent rise in the aggregation capability of washed platelets with Ristocetin was of decisive importance in the reproducibility of the rather sophisticated technique of determining the Ristocetin cofactor. The addition of EDTA largely inhabited this effect. Even in the group of patients with \"severe\" v.W.d., part of the findings obtained with screening tests of the intrinsic coagulation system remained within the normal range. There was no statistical correlation between the individual laboratory findings in the group of healthy persons. In the group with \"mild\" v.W.d., there was a significant correlation between platelet adhesiveness and Ristocetin-induced platelet aggregation on the one hand, and platelet adhesiveness and the Ristocetin cofactor on the other. In addition, a verified correlation was found to exist between Ristocetin-induced platelet aggregation and Ristocetin cofactor and between functional factor VIII and factor VIII-related antigen. In the group of \"severe\" v.W.d., only platelet adhesiveness and functional factor VIII were not significantly correlated, whilst all other correlations were of statistical significance. Further problems involved in the diagnosis of v.W.d., such as the variablility of laboratory findings, the existence of subgroups, as well as the difficulties involved in the statistical evaluation of individual cases suffering from v.W.d. are discussed."}
{"id": "PMID:1086552", "title": "[Alpha-1-antitrypsin in children with glomerular diseases (author's transl)].", "content": "Alpha-1-antitrypsin was determined in children with glomerular diseases by means of a quantitative radial immunodiffusion method. The concentration of this inhibiting protein has been found to be very low during relapses. An attempt has been made to correlate this finding with the clinical picture and the presumed underlying pathological mechanism. The loss of this inhibitor due to proteinuria is one of the explanations, in concurrence with the findings of other authors. The second explanation lies in the consumption of the inhibitor protein as a consequence of the reaction with liberated proteolytic enzymes.", "contents": "[Alpha-1-antitrypsin in children with glomerular diseases (author's transl)]. Alpha-1-antitrypsin was determined in children with glomerular diseases by means of a quantitative radial immunodiffusion method. The concentration of this inhibiting protein has been found to be very low during relapses. An attempt has been made to correlate this finding with the clinical picture and the presumed underlying pathological mechanism. The loss of this inhibitor due to proteinuria is one of the explanations, in concurrence with the findings of other authors. The second explanation lies in the consumption of the inhibitor protein as a consequence of the reaction with liberated proteolytic enzymes."}
{"id": "PMID:1086546", "title": "[Significance of changesin the topography of the superior cerebellar artery in the angiographic diagnosis of tumors of the posterior cranial fossa (II)].", "content": "Typical shifts of the superior cerebellar artery and its segments were studied in 67 vertebral angiogrammes showing 33 acoustic neurinomas, 7 meningiomas of the cerebellopontile angle, 13 cerebellar hemispheres tumours, 10 pontile tumours and 4 meningiomas of the free margin of the tentorium. The rationale of accounting for the angiographic symptom complexes typical for acoustic neurinomas with varying directions of growth were singled out.", "contents": "[Significance of changesin the topography of the superior cerebellar artery in the angiographic diagnosis of tumors of the posterior cranial fossa (II)]. Typical shifts of the superior cerebellar artery and its segments were studied in 67 vertebral angiogrammes showing 33 acoustic neurinomas, 7 meningiomas of the cerebellopontile angle, 13 cerebellar hemispheres tumours, 10 pontile tumours and 4 meningiomas of the free margin of the tentorium. The rationale of accounting for the angiographic symptom complexes typical for acoustic neurinomas with varying directions of growth were singled out."}
{"id": "PMID:1086553", "title": "[Possibilities of the immunological in vitro-diagnostic in nonspecific lung diseases (author's transl)].", "content": "Immunological in vitro-methods can be used in the improved diagnostic of nonspecific lung diseases. A short review is given about the recommended tests in the diagnostic of the four types of immunological reactions responsible for allergic disorders of the lungs (IgE-mediated immediate-reaction, cytotoxic-reaction, Arthus-reaction and cell-mediated hypersensitivity), the alpha1-antitrypsin deficiency and the immunological defence in the bronchial mucosa.", "contents": "[Possibilities of the immunological in vitro-diagnostic in nonspecific lung diseases (author's transl)]. Immunological in vitro-methods can be used in the improved diagnostic of nonspecific lung diseases. A short review is given about the recommended tests in the diagnostic of the four types of immunological reactions responsible for allergic disorders of the lungs (IgE-mediated immediate-reaction, cytotoxic-reaction, Arthus-reaction and cell-mediated hypersensitivity), the alpha1-antitrypsin deficiency and the immunological defence in the bronchial mucosa."}
{"id": "PMID:1086554", "title": "[Effects of brain extract and hydrolysate on nerve tissue in vitro].", "content": "Explants of PNS and CNS of chick embryos or fetal rats were cultivated and brainhydrolysat cerebrolysin or a synthetic aminoacidsolution or a brain extrakt was applied to the cultures. Cerebrolysin exercised in vitro an effect, which is related to charge only trifling. The cerebrolysin effect is essential related to the concentration/medium. High concentration of cerebrolysin decreases the growth of nerve fibers. Migration of non-neuronal cells increases. A stimulation of nerve fibers growth resembling to NGF was not seen. The density of cells or number of cells/volume unit, rate of mitosis and phases of mitotic non-neuronal cells is changed by cerebrolysin. The effect of cerebrolysin in vitro is compared to the tested aminoacidsolution and brainextract resembling. NGF may be important selectiv for stimulation of the activity of sympathetic and sensoric ganglia. Brainextract and brainhydrolysat and aminoacidsolution influenced in vitro PNS- and CNS-tissue cultures.", "contents": "[Effects of brain extract and hydrolysate on nerve tissue in vitro]. Explants of PNS and CNS of chick embryos or fetal rats were cultivated and brainhydrolysat cerebrolysin or a synthetic aminoacidsolution or a brain extrakt was applied to the cultures. Cerebrolysin exercised in vitro an effect, which is related to charge only trifling. The cerebrolysin effect is essential related to the concentration/medium. High concentration of cerebrolysin decreases the growth of nerve fibers. Migration of non-neuronal cells increases. A stimulation of nerve fibers growth resembling to NGF was not seen. The density of cells or number of cells/volume unit, rate of mitosis and phases of mitotic non-neuronal cells is changed by cerebrolysin. The effect of cerebrolysin in vitro is compared to the tested aminoacidsolution and brainextract resembling. NGF may be important selectiv for stimulation of the activity of sympathetic and sensoric ganglia. Brainextract and brainhydrolysat and aminoacidsolution influenced in vitro PNS- and CNS-tissue cultures."}
{"id": "PMID:1086566", "title": "Trace elements in tissues of normal and vitamin D2-treated rats.", "content": "Comparative spectroscopic investigations were performed on 22 trace elements regarding their presence and quantitative relations in cartilages (costal cartilage, intervertebral disc) and in soft tissues most exposed to metastatic calcification (aorta, kidney) of normal, vitamin D2-treated and rachitic rats. The results revealed differences in concentration and distribution of trace elements between soft tissues and cartilages on the one hand, and untreated controls and vitamin D-treated rats on the other. On vitamin D treatment an increase in the Cr, Fe and Cu contents was noted in the soft tissues, whereas in cartilages a higher Zn content was shown. It is suggested that these trace elements may play an important role in the mechanism of calcification, some acting as challengers, others as adjuvants or inhibitors.", "contents": "Trace elements in tissues of normal and vitamin D2-treated rats. Comparative spectroscopic investigations were performed on 22 trace elements regarding their presence and quantitative relations in cartilages (costal cartilage, intervertebral disc) and in soft tissues most exposed to metastatic calcification (aorta, kidney) of normal, vitamin D2-treated and rachitic rats. The results revealed differences in concentration and distribution of trace elements between soft tissues and cartilages on the one hand, and untreated controls and vitamin D-treated rats on the other. On vitamin D treatment an increase in the Cr, Fe and Cu contents was noted in the soft tissues, whereas in cartilages a higher Zn content was shown. It is suggested that these trace elements may play an important role in the mechanism of calcification, some acting as challengers, others as adjuvants or inhibitors."}
{"id": "PMID:1086567", "title": "[Radioimmunological demonstration of DNA-specific antibodies].", "content": "Using 125I chemically labelled denatured (d) and native (n) DNA, specifically binding antibodies were demonstrated in the sera of Lupus erythemathodes patients by means of the Farr technique. (NH4)2SO4 was used to separate the immunologically bound 125I-d-DNA. For 125I-n-DNA the use of a secondary antiserum for the precipitation of the primary immune complex is advantageous. The influence of antigen concentration upon the binding rate was studied. Titre determinations can be made with the proposed method.", "contents": "[Radioimmunological demonstration of DNA-specific antibodies]. Using 125I chemically labelled denatured (d) and native (n) DNA, specifically binding antibodies were demonstrated in the sera of Lupus erythemathodes patients by means of the Farr technique. (NH4)2SO4 was used to separate the immunologically bound 125I-d-DNA. For 125I-n-DNA the use of a secondary antiserum for the precipitation of the primary immune complex is advantageous. The influence of antigen concentration upon the binding rate was studied. Titre determinations can be made with the proposed method."}
{"id": "PMID:1086568", "title": "[L-carnitine as a basis of cholinomimetic substances].", "content": "Acetylcarnitine, though having the same configuration as acetylcholine and Acetyl-beta-methylcholine, is devoid of cholinomimetic properties as long as the carboxylic group is free. Contrary findings are explainable by the lack of uniformity of the test substance, caused by substitution of the carboxylic group and intramolecular cleavage of water or acetic acid from carnitine or acetylcarnitine and by admixtures of active substances, and are attributable to the formation of metabolites in vivo. Already the recrystallization of salts of L-acetylcarnitine and L-carnitine in alcohols causes the formation of active carboxylic esters. The latter can be separated and identified by t.l.c. from the starting substances. At the isolated frog heart (Rana esculenta), neither L-carnitine nor L-acetylcarnitine have muscarine-like effects; higher concentrations of them (0.03-0.15 M) exert positively inotropic effects that increase with concentration and are qualitatively and quantitatively equal for L-carnitine and lower O-acyl-L-carnitines. As betaine, L-carnitine affects the heart rate only at 42 +/- 12 mg/ml, crotonic acid betaine at 22 +/- 7 mg/ml, gamma-butyrobetaine at 15 +/- 8 mg/ml. As a result of carboxyl substitution of betaines, the cholinomimetic properties increase to the level of the stimulation system choline/acetylcholine. The LD50 of L-acetylcarnitine for mice injected s.c. with 8.4 (7.3-9.7) mg/g body weight is within the range of LD50 of L-carnitine. Both substances, even when administered in high doses, give no such symptoms as cholinomimetic substances. Carnitine carboxyl ester, acetylcarnitine carboxyl ester, and other carnitine derivatives, on a molar basis, are 2-10(1) to 2-10(3)-fold more toxic than carnitine and acetylcarnitine. The modes of action of carnitines and their metabolites upon the heart rate are discussed.", "contents": "[L-carnitine as a basis of cholinomimetic substances]. Acetylcarnitine, though having the same configuration as acetylcholine and Acetyl-beta-methylcholine, is devoid of cholinomimetic properties as long as the carboxylic group is free. Contrary findings are explainable by the lack of uniformity of the test substance, caused by substitution of the carboxylic group and intramolecular cleavage of water or acetic acid from carnitine or acetylcarnitine and by admixtures of active substances, and are attributable to the formation of metabolites in vivo. Already the recrystallization of salts of L-acetylcarnitine and L-carnitine in alcohols causes the formation of active carboxylic esters. The latter can be separated and identified by t.l.c. from the starting substances. At the isolated frog heart (Rana esculenta), neither L-carnitine nor L-acetylcarnitine have muscarine-like effects; higher concentrations of them (0.03-0.15 M) exert positively inotropic effects that increase with concentration and are qualitatively and quantitatively equal for L-carnitine and lower O-acyl-L-carnitines. As betaine, L-carnitine affects the heart rate only at 42 +/- 12 mg/ml, crotonic acid betaine at 22 +/- 7 mg/ml, gamma-butyrobetaine at 15 +/- 8 mg/ml. As a result of carboxyl substitution of betaines, the cholinomimetic properties increase to the level of the stimulation system choline/acetylcholine. The LD50 of L-acetylcarnitine for mice injected s.c. with 8.4 (7.3-9.7) mg/g body weight is within the range of LD50 of L-carnitine. Both substances, even when administered in high doses, give no such symptoms as cholinomimetic substances. Carnitine carboxyl ester, acetylcarnitine carboxyl ester, and other carnitine derivatives, on a molar basis, are 2-10(1) to 2-10(3)-fold more toxic than carnitine and acetylcarnitine. The modes of action of carnitines and their metabolites upon the heart rate are discussed."}
{"id": "PMID:1086569", "title": "Studies on column fractionation of immune cells. VI. Separation of thymus-derived lymphocytes by means of protein beads coated with antigen-antibody complexes.", "content": "Calf serum beads coated with antigen-antibody complexes were used as cellular immuno-adsorbents to separate mouse T-lymphocytes with a purity of more than 90%. The beads coated by means of glutaraldehyde could be used at least three times without loss of cell-binding capacity.", "contents": "Studies on column fractionation of immune cells. VI. Separation of thymus-derived lymphocytes by means of protein beads coated with antigen-antibody complexes. Calf serum beads coated with antigen-antibody complexes were used as cellular immuno-adsorbents to separate mouse T-lymphocytes with a purity of more than 90%. The beads coated by means of glutaraldehyde could be used at least three times without loss of cell-binding capacity."}
{"id": "PMID:1086563", "title": "[Organization of the rhythmic activity of the spinal center motoneurons of the lymphatic hearts of Rana temporaria frogs].", "content": "Spontaneous rhythmic impulse discharges of motoneurons of spinal centers of the posterior lymphatic hearts have been recorded from the ventral roots of isolated spinal cord perfused by oxygenated Ringer's solution. Inhibition of the transmission in interneuronal synapses evoked by abolition of Ca ions from the external solution and by the addition to the latter of 1--4 mM EDTA was accompanied by the block of the spontaneous impulse activity. Blocking of rhythmic efferent discharges in the anterior roots was also observed after the addition to normal Ringer's solution of 10--30 mM MgCl2. Inhibition of the spontaneous activity by high Mg content in the perfusion fluid could be alleviated by the addition of 5--15 mM CaCl2 to this solution. Antidromic impulses in the ventral roots of the XI and X segments, evoked by rhythmic electrical stimulation of these roots, did not affect the intrinsic rhythm of motor discharges.", "contents": "[Organization of the rhythmic activity of the spinal center motoneurons of the lymphatic hearts of Rana temporaria frogs]. Spontaneous rhythmic impulse discharges of motoneurons of spinal centers of the posterior lymphatic hearts have been recorded from the ventral roots of isolated spinal cord perfused by oxygenated Ringer's solution. Inhibition of the transmission in interneuronal synapses evoked by abolition of Ca ions from the external solution and by the addition to the latter of 1--4 mM EDTA was accompanied by the block of the spontaneous impulse activity. Blocking of rhythmic efferent discharges in the anterior roots was also observed after the addition to normal Ringer's solution of 10--30 mM MgCl2. Inhibition of the spontaneous activity by high Mg content in the perfusion fluid could be alleviated by the addition of 5--15 mM CaCl2 to this solution. Antidromic impulses in the ventral roots of the XI and X segments, evoked by rhythmic electrical stimulation of these roots, did not affect the intrinsic rhythm of motor discharges."}
{"id": "PMID:1086564", "title": "[Comparison of the protein composition of the chromatins of several species of vertebrates].", "content": "Protein composition of chromatins from the liver of various Vertebrates (frog, turtle, hen and rat) has been compared by the immunological method of double diffusion in gel. It was shown that non-histone chromosomal proteins and histones exhibit evident species specificity.", "contents": "[Comparison of the protein composition of the chromatins of several species of vertebrates]. Protein composition of chromatins from the liver of various Vertebrates (frog, turtle, hen and rat) has been compared by the immunological method of double diffusion in gel. It was shown that non-histone chromosomal proteins and histones exhibit evident species specificity."}
{"id": "PMID:1086565", "title": "[Restoration of the behavioral reactions of Rana esculenta frogs during regeneration of visual nerve fibers].", "content": "After sectioning the optic nerve, various forms of visually guided behaviour in the frog R. esculenta recover unevenly. Within 16--20 days, the onset of reactions was observed which are associated with perception of relatively large unmoving objects during active locomotion of animals (1); within 55 days -- avoidance reactions (2); within 90 days -- feeling reactions (3) were formed. In electrophysiological experiments it was shown that axons of the ganglionic retinal cells from various classes form contacts with the tectum also at different periods. The data obtained confirm the significance of specialized \"canalization\" of the retino--central nervous systems of transmission and evaluation of visual information for organization of separate forms of visually monitored behaviour in frogs.", "contents": "[Restoration of the behavioral reactions of Rana esculenta frogs during regeneration of visual nerve fibers]. After sectioning the optic nerve, various forms of visually guided behaviour in the frog R. esculenta recover unevenly. Within 16--20 days, the onset of reactions was observed which are associated with perception of relatively large unmoving objects during active locomotion of animals (1); within 55 days -- avoidance reactions (2); within 90 days -- feeling reactions (3) were formed. In electrophysiological experiments it was shown that axons of the ganglionic retinal cells from various classes form contacts with the tectum also at different periods. The data obtained confirm the significance of specialized \"canalization\" of the retino--central nervous systems of transmission and evaluation of visual information for organization of separate forms of visually monitored behaviour in frogs."}
{"id": "PMID:1086574", "title": "May Trichomonas vaginalis provoke conjunctivitis?", "content": "In the world literature we have found descriptions of five cases of Trichomonas vaginalis in the conjunctiva. Conjunctival swabs taken from 122 patients with conjunctivitis or keratitis revealed no flagellate micro-organisms on phase-contrast microscopy and culture. Neither did conjunctival swabs from 272 newborn infants, in spite of the fact that 3.7 per cent of their mothers had Trichomonas in the vagina. Trichomonas vaginalis is hardly likely to be responsible for conjunctivitis.", "contents": "May Trichomonas vaginalis provoke conjunctivitis? In the world literature we have found descriptions of five cases of Trichomonas vaginalis in the conjunctiva. Conjunctival swabs taken from 122 patients with conjunctivitis or keratitis revealed no flagellate micro-organisms on phase-contrast microscopy and culture. Neither did conjunctival swabs from 272 newborn infants, in spite of the fact that 3.7 per cent of their mothers had Trichomonas in the vagina. Trichomonas vaginalis is hardly likely to be responsible for conjunctivitis."}
{"id": "PMID:1086575", "title": "Examination of colour vision by use of induced contrast colours. Design of a new series of tissue paper contrast tests.", "content": "The ability to induce contrast colours is evident in normal persons by the tissue paper contrast principle. However, tests of good quality are not easily available. The design of a new series of charts follows two principles: 1) Selection of background hues in accordance with the maximally desaturated regions of the spectrum as seen by the colour defectives. 2) Exact adjustment of the neutral test field (constituted by the chart figures) in order to eliminate any false clue due to brightness contrasts. By introducing chart figures of alternative grey values appropriate tests can be attained for each type of colour vision defect. 37 persons with congenital colour defect and 15 persons with acquired defects were examined. The charts, according to the criteria for selection, proved to be selective in their screening efficiency.", "contents": "Examination of colour vision by use of induced contrast colours. Design of a new series of tissue paper contrast tests. The ability to induce contrast colours is evident in normal persons by the tissue paper contrast principle. However, tests of good quality are not easily available. The design of a new series of charts follows two principles: 1) Selection of background hues in accordance with the maximally desaturated regions of the spectrum as seen by the colour defectives. 2) Exact adjustment of the neutral test field (constituted by the chart figures) in order to eliminate any false clue due to brightness contrasts. By introducing chart figures of alternative grey values appropriate tests can be attained for each type of colour vision defect. 37 persons with congenital colour defect and 15 persons with acquired defects were examined. The charts, according to the criteria for selection, proved to be selective in their screening efficiency."}
{"id": "PMID:1086571", "title": "[Surface structure of human T and B lymphocytes forming rosettes with erythrocytes in scanning microscopy].", "content": "The authors tried to evaluate the surface of T and B cells in scanning microscope. The lymphocytes were isolated from the blood of healthy men and separated into T and B cells on the basis of rosette tests E and EAC. About 80% of T cells in type E rosettes showed marked bulgings of surface corresponding to numerous, densely crowded microvilli. The remaining 20% of lymphocytes T had more smooth surface. Difficulties were observed frequently in finding differences in the appearance of these cells in relation to lymphocytes B in type EAC rosettes whose surface was covered with minute bulgings corresponding to more delicate and less densely arranged microvilli. The differences in the structure of T and B cells after formation of E and EAC rosettes were not so evident that a decisive separation of both these cell populations on morphological basis would be possible.", "contents": "[Surface structure of human T and B lymphocytes forming rosettes with erythrocytes in scanning microscopy]. The authors tried to evaluate the surface of T and B cells in scanning microscope. The lymphocytes were isolated from the blood of healthy men and separated into T and B cells on the basis of rosette tests E and EAC. About 80% of T cells in type E rosettes showed marked bulgings of surface corresponding to numerous, densely crowded microvilli. The remaining 20% of lymphocytes T had more smooth surface. Difficulties were observed frequently in finding differences in the appearance of these cells in relation to lymphocytes B in type EAC rosettes whose surface was covered with minute bulgings corresponding to more delicate and less densely arranged microvilli. The differences in the structure of T and B cells after formation of E and EAC rosettes were not so evident that a decisive separation of both these cell populations on morphological basis would be possible."}
{"id": "PMID:1086577", "title": "The immunological function of the adenoid. The proportions of T and B cells.", "content": "The proportions of T and B cells in lymphocyte suspensions prepared from peripheral blood and adenoids from children undergoing adenoidectomy were determined, using Ig fluorescence and E rosetting techniques. The proportion of B cells is higher in the adenoid than in the blood, and the proportion of T cells is correspondingly lower. Preliminary results of stimulations of adenoid lymphocytes by antural antigens, such as N. catarrhalis, indicate that both mitogenic and antigenic responses are induced. Separation of lymphocyte suspensions on leucopac, and subsequent stimulation by N. catarrhalis and PHA result in abolition of the response to N. catarrhalis, indicating that reaction to this natural antigen is a B cell function.", "contents": "The immunological function of the adenoid. The proportions of T and B cells. The proportions of T and B cells in lymphocyte suspensions prepared from peripheral blood and adenoids from children undergoing adenoidectomy were determined, using Ig fluorescence and E rosetting techniques. The proportion of B cells is higher in the adenoid than in the blood, and the proportion of T cells is correspondingly lower. Preliminary results of stimulations of adenoid lymphocytes by antural antigens, such as N. catarrhalis, indicate that both mitogenic and antigenic responses are induced. Separation of lymphocyte suspensions on leucopac, and subsequent stimulation by N. catarrhalis and PHA result in abolition of the response to N. catarrhalis, indicating that reaction to this natural antigen is a B cell function."}
{"id": "PMID:1086576", "title": "Immunodeficiency syndromes with otorhinolaryngological manifestations.", "content": "Among patients with recurrent, protracted or chronic infections of the respiratory tract involving the middle ear, 18 were found to have immunodeficiencies. In 10 of the patients, deficiency of immunoglobulins belonging to the IgG, IgA and IgM classes was found. Seven patients had an isolated IgA deficiency. One patient had a combined immunodeficiency with defects of the T-cell system and the B-cell system. One patient had an isolated T-cell deficiency.", "contents": "Immunodeficiency syndromes with otorhinolaryngological manifestations. Among patients with recurrent, protracted or chronic infections of the respiratory tract involving the middle ear, 18 were found to have immunodeficiencies. In 10 of the patients, deficiency of immunoglobulins belonging to the IgG, IgA and IgM classes was found. Seven patients had an isolated IgA deficiency. One patient had a combined immunodeficiency with defects of the T-cell system and the B-cell system. One patient had an isolated T-cell deficiency."}
{"id": "PMID:1086578", "title": "The vestibular aqueduct in Meniere's disease.", "content": "The vestibular aquaeduct was evaluated blindly in tomograms from patients with Meniere's disease or chronic otitis and from patients without ear disorders. The descending part of the aquaeduct was clearly visible in 95% of the patients without ear disease. In the two other groups, it was visible only in about 50%. It is suggested that a narrow or invisible vestibular aquaeduct is an unspecific radiological sign in a variety of ear diseases.", "contents": "The vestibular aqueduct in Meniere's disease. The vestibular aquaeduct was evaluated blindly in tomograms from patients with Meniere's disease or chronic otitis and from patients without ear disorders. The descending part of the aquaeduct was clearly visible in 95% of the patients without ear disease. In the two other groups, it was visible only in about 50%. It is suggested that a narrow or invisible vestibular aquaeduct is an unspecific radiological sign in a variety of ear diseases."}
{"id": "PMID:1086583", "title": "Non-hyperbolic force-velocity relationship in single muscle fibres.", "content": "The force-velocity relation has been studied in sixteen single fibres from frog semitendinosus muscle with particular attention to the high-force portion of the curve. The force-velocity curve was hyperbolic except for a reversal of curvature near 80% measured isometric tension (PO). Rectangular hyperbolas fitted (linear, least-squares method) these data well only when values below 0.78 PO were considered. Extrapolation of these hyperbolas above 0.78 PO gave predicted isometric tensions (P*O) which averaged 32+/-6% above the measured PO values. Hill's constants (1.84 degrees C) for these hyperbolas were: a/P*O=0.177+/-0.021, b=0.329+/- 0.035 M.L./sec, Vmax=1.91+/-0.074 M.L./sec. The reversal of curvature persisted when force-velocity data were obtained using: 1 or 60 min response intervals, afterloaded isotonic responses, grid stimulation, electrically induced contractures and bundles of fibres. The reversal of curvature diminished when force-velocity data were obtained from slightly stretched fibres (about 2.3 mum sarcomere length as compared to 2.1 mum in the control). The results indicate that sarcomere length redistributions probably do not account for the non-hyperbolic force-velocity relation. An explanation for the behavior based on the geometry of the contractile filament lattice is discussed.", "contents": "Non-hyperbolic force-velocity relationship in single muscle fibres. The force-velocity relation has been studied in sixteen single fibres from frog semitendinosus muscle with particular attention to the high-force portion of the curve. The force-velocity curve was hyperbolic except for a reversal of curvature near 80% measured isometric tension (PO). Rectangular hyperbolas fitted (linear, least-squares method) these data well only when values below 0.78 PO were considered. Extrapolation of these hyperbolas above 0.78 PO gave predicted isometric tensions (P*O) which averaged 32+/-6% above the measured PO values. Hill's constants (1.84 degrees C) for these hyperbolas were: a/P*O=0.177+/-0.021, b=0.329+/- 0.035 M.L./sec, Vmax=1.91+/-0.074 M.L./sec. The reversal of curvature persisted when force-velocity data were obtained using: 1 or 60 min response intervals, afterloaded isotonic responses, grid stimulation, electrically induced contractures and bundles of fibres. The reversal of curvature diminished when force-velocity data were obtained from slightly stretched fibres (about 2.3 mum sarcomere length as compared to 2.1 mum in the control). The results indicate that sarcomere length redistributions probably do not account for the non-hyperbolic force-velocity relation. An explanation for the behavior based on the geometry of the contractile filament lattice is discussed."}
{"id": "PMID:1086587", "title": "LDH isoenzymes and myocardial infarction in patients undergoing coronary bypass surgery: an excellent correlation.", "content": "To evaluate the efficacy of LDH isoenzymes in the detection of myocardial infarction in patients undergoing coronary bypass surgery, 73 patients were studied pre- and postoperatively by ECG, CPK, SGOT, total serum LDH, and LDH isoenzyme measurements. A reversal of the LDH1:LDH2 ratio was considered indicative of myocardial necrosis. Accordingly, the patients were separated into two groups: Group A (23 patients) who demonstrated an LDH1:LDH2 ratio exceeding 1.0 and Group B (50 patients) who failed to reveal an LDH1;LDH2 reversal. The two groups were similar in regard to preoperative evaluation and operative procedure performed. The postoperative ECG findings were significantly different. In Group A 18 of 23 patients (78 per cent) developed significant new Q waves. This occurred in only one patient in Group B. Significant arrhythmias occurred in 70 per cent of the patients in Group A as compared to 14 per cent of those patients in Group B. Severe congestive heart failure and/or clinical evidence of shock occurred in 39 per cent of Group A patients and in none in Group B. The results of our study indicate that the reversal of the LDH1:LDH2 ratio is a valuable tool for the evaluation of postoperative myocardial infarction.", "contents": "LDH isoenzymes and myocardial infarction in patients undergoing coronary bypass surgery: an excellent correlation. To evaluate the efficacy of LDH isoenzymes in the detection of myocardial infarction in patients undergoing coronary bypass surgery, 73 patients were studied pre- and postoperatively by ECG, CPK, SGOT, total serum LDH, and LDH isoenzyme measurements. A reversal of the LDH1:LDH2 ratio was considered indicative of myocardial necrosis. Accordingly, the patients were separated into two groups: Group A (23 patients) who demonstrated an LDH1:LDH2 ratio exceeding 1.0 and Group B (50 patients) who failed to reveal an LDH1;LDH2 reversal. The two groups were similar in regard to preoperative evaluation and operative procedure performed. The postoperative ECG findings were significantly different. In Group A 18 of 23 patients (78 per cent) developed significant new Q waves. This occurred in only one patient in Group B. Significant arrhythmias occurred in 70 per cent of the patients in Group A as compared to 14 per cent of those patients in Group B. Severe congestive heart failure and/or clinical evidence of shock occurred in 39 per cent of Group A patients and in none in Group B. The results of our study indicate that the reversal of the LDH1:LDH2 ratio is a valuable tool for the evaluation of postoperative myocardial infarction."}
{"id": "PMID:1086584", "title": "\"Warm\" E rosettes as an index of activation of T lymphocytes.", "content": "Rosetting at 37 degrees (\"warm\" rosettes) was assayed as a technique to determine the degree of activation of T lymphocytes. Standard \"cold\" rosettes and an index of \"warm\" to \"cold\" rosettes were used to correct possible changes in the total percentage of T cells. Statistically higher numbers of \"warm\" rosettes were obtained in Pokeweed mitogen stimulated cultures when compared with unstimulated control; but not in old tuberculin stimulated cultures of lymphocytes from PPD positive individuals when compared with those of PPD negative. Also a significant rise was found in control cultures of 3 and 5 days when compared with those of day 1. We think that \"warm\" rosetting may be a useful technique for determining the degree of immunological activation of a lymphocyte population.", "contents": "\"Warm\" E rosettes as an index of activation of T lymphocytes. Rosetting at 37 degrees (\"warm\" rosettes) was assayed as a technique to determine the degree of activation of T lymphocytes. Standard \"cold\" rosettes and an index of \"warm\" to \"cold\" rosettes were used to correct possible changes in the total percentage of T cells. Statistically higher numbers of \"warm\" rosettes were obtained in Pokeweed mitogen stimulated cultures when compared with unstimulated control; but not in old tuberculin stimulated cultures of lymphocytes from PPD positive individuals when compared with those of PPD negative. Also a significant rise was found in control cultures of 3 and 5 days when compared with those of day 1. We think that \"warm\" rosetting may be a useful technique for determining the degree of immunological activation of a lymphocyte population."}
{"id": "PMID:1086588", "title": "Vascular and cardiac contractile reserve in the dog heart with chronic multiple coronary occlusions.", "content": "Nineteen mongrel dogs survived chronic occlusion of the left circumflex and of the right coronary artery without infarction due to the timely development of a collateral circulation. Only 38 per cent of the conductance of the arteries before occlusion was restored by collateral vessels. In these animals and in 15 control dogs with normal coronary arteries myocardial contractility, contractility reserve, and myocardial blood flow were studied. The same was done in dogs with chronic coronary artery occlusion after aortocoronary bypass. Myocardial blood flow was determined woth the tracer microsphere technique. Contractility reserve was tested and defined as isovolumetric left ventricular pressure and dp/dt max with norepinephrine infusion and cross-clamping of the aorta. Contractile reserve was not significantly different between normal dogs and dogs with chronic coronary artery occlusion before and after aortocoronary bypass. Myocardial blood flow during control conditions was homogenously distributed in all three groups studied. The ratio of blood flow to the endocardium and the epicardium was not significantly different from inity. Coronary reserve was determined at peak reactive hyperemia following a 20 second period of coronary artery occlusion, with ongoing norepinephrine infusion. Under these conditions subendocardial fow in normal dogs rose by a factor of 7.9 while subepicardial flow increased 7.4 times. In dogs with chronic occlusion of two coronary arteries the increase of myocardial flow was nonnomogenous; subendocardial flow to areas supplied by a normal coronary artery rose by a factor of 7.0 while subepicardial flow increased 5.7 times control. Subendocardial collateral flow rose by a factor of 2.4 and subepicardial collateral flow increased 3.5 times control. In normal dogs norepinephrine alone did not result in maximal coronary flow but only 57 per cent thereof. Dogs with chronic coronary occlusion, however, required the entire coronary reserve in areas that were supplied by a normal coronary artery, whereas areas supplied by collaterals became ischemic. Opening of an aortocoronary bypass restored normal flow to previously ischemic areas, and reduced the flow to areas supplied by a normal artery. With the bypass open no differences existed between normal dogs and those with two occluded coronary arteries. We conclude that the norepinephrine-stimulated contractile reserve of hearts with chronic coronary occlusion was comparable to that of normal hearts; however, norepinephrine forced these hearts to spend the entire flow reserve of the remaining normal artery while producing ischemia in collateral-dependent areas. The same dose of norepinephrine did not require the entire flow reserve of normal dogs.", "contents": "Vascular and cardiac contractile reserve in the dog heart with chronic multiple coronary occlusions. Nineteen mongrel dogs survived chronic occlusion of the left circumflex and of the right coronary artery without infarction due to the timely development of a collateral circulation. Only 38 per cent of the conductance of the arteries before occlusion was restored by collateral vessels. In these animals and in 15 control dogs with normal coronary arteries myocardial contractility, contractility reserve, and myocardial blood flow were studied. The same was done in dogs with chronic coronary artery occlusion after aortocoronary bypass. Myocardial blood flow was determined woth the tracer microsphere technique. Contractility reserve was tested and defined as isovolumetric left ventricular pressure and dp/dt max with norepinephrine infusion and cross-clamping of the aorta. Contractile reserve was not significantly different between normal dogs and dogs with chronic coronary artery occlusion before and after aortocoronary bypass. Myocardial blood flow during control conditions was homogenously distributed in all three groups studied. The ratio of blood flow to the endocardium and the epicardium was not significantly different from inity. Coronary reserve was determined at peak reactive hyperemia following a 20 second period of coronary artery occlusion, with ongoing norepinephrine infusion. Under these conditions subendocardial fow in normal dogs rose by a factor of 7.9 while subepicardial flow increased 7.4 times. In dogs with chronic occlusion of two coronary arteries the increase of myocardial flow was nonnomogenous; subendocardial flow to areas supplied by a normal coronary artery rose by a factor of 7.0 while subepicardial flow increased 5.7 times control. Subendocardial collateral flow rose by a factor of 2.4 and subepicardial collateral flow increased 3.5 times control. In normal dogs norepinephrine alone did not result in maximal coronary flow but only 57 per cent thereof. Dogs with chronic coronary occlusion, however, required the entire coronary reserve in areas that were supplied by a normal coronary artery, whereas areas supplied by collaterals became ischemic. Opening of an aortocoronary bypass restored normal flow to previously ischemic areas, and reduced the flow to areas supplied by a normal artery. With the bypass open no differences existed between normal dogs and those with two occluded coronary arteries. We conclude that the norepinephrine-stimulated contractile reserve of hearts with chronic coronary occlusion was comparable to that of normal hearts; however, norepinephrine forced these hearts to spend the entire flow reserve of the remaining normal artery while producing ischemia in collateral-dependent areas. The same dose of norepinephrine did not require the entire flow reserve of normal dogs."}
{"id": "PMID:1086585", "title": "Immunosuppressive effect of corticosteroids on rabbit's humoral and cellular response.", "content": "The immunosuppressive effect of two of the most used steroids, 16-Methylen-prednisolone and betamethasone, was studied in rabbits sensitized to bovine serum albumin (BSA). In the steroid treated animals the hemagglutination and precipitation titres obtained in Boyden's test and gel-diffusion respectively, were strongly decreased as compared with the controls. The Arthus reaction was also inhibited. No response was detected in the lymphocyte transformation test (LTT) against the antigen (BSA) in the treated animals, and also the PHA induced transformation was suppressed but not completely abolished. A remarked decrease in the number of circulating mononuclear cells was observed in the treated animals. The number of T rosette-forming cells was also strongly decreased, but the percentage of B cells bearing IgG on their membrane detected by immunofluorescence offered only a slight decrease as compared with the controls. All these changes were observed earlier and more intensely in the Betamethasone treated rabbits.", "contents": "Immunosuppressive effect of corticosteroids on rabbit's humoral and cellular response. The immunosuppressive effect of two of the most used steroids, 16-Methylen-prednisolone and betamethasone, was studied in rabbits sensitized to bovine serum albumin (BSA). In the steroid treated animals the hemagglutination and precipitation titres obtained in Boyden's test and gel-diffusion respectively, were strongly decreased as compared with the controls. The Arthus reaction was also inhibited. No response was detected in the lymphocyte transformation test (LTT) against the antigen (BSA) in the treated animals, and also the PHA induced transformation was suppressed but not completely abolished. A remarked decrease in the number of circulating mononuclear cells was observed in the treated animals. The number of T rosette-forming cells was also strongly decreased, but the percentage of B cells bearing IgG on their membrane detected by immunofluorescence offered only a slight decrease as compared with the controls. All these changes were observed earlier and more intensely in the Betamethasone treated rabbits."}
{"id": "PMID:1086592", "title": "Quantitation of \"acute-phase proteins\" postoperatively. Value in detection and monitoring of complications.", "content": "Serial determinations of serum C-reactive protein are helpful in the detection and monitoring of postoperative complications associated with inflammation and/or tissue necrosis. The serum C-reactive protein level begins to increase within sex hours after operation, peaks on the second day, and by the third postoperative day begins to decrease toward the preoperative level. In cases with surgical complications involving inflammation, serum C-reactive protein levels remain elevated and do not show a decline on the third postoperative day. Serum levels of other \"acute-phase proteins,\" such as alpha-1 acid glycoprotein, ceruloplasmin, alpha-1 antitrypsin, and haptoglobin, were found to increase in response to surgical procedures, but subsequent to the increase in C-reactive protein. These other proteins offer no additional information in monitoring the postoperative acutephase response,", "contents": "Quantitation of \"acute-phase proteins\" postoperatively. Value in detection and monitoring of complications. Serial determinations of serum C-reactive protein are helpful in the detection and monitoring of postoperative complications associated with inflammation and/or tissue necrosis. The serum C-reactive protein level begins to increase within sex hours after operation, peaks on the second day, and by the third postoperative day begins to decrease toward the preoperative level. In cases with surgical complications involving inflammation, serum C-reactive protein levels remain elevated and do not show a decline on the third postoperative day. Serum levels of other \"acute-phase proteins,\" such as alpha-1 acid glycoprotein, ceruloplasmin, alpha-1 antitrypsin, and haptoglobin, were found to increase in response to surgical procedures, but subsequent to the increase in C-reactive protein. These other proteins offer no additional information in monitoring the postoperative acutephase response,"}
{"id": "PMID:1086594", "title": "Cellulitis due to Haemophilus influenzae type B. Antigenemia and antibody responses.", "content": "The techniques of serum countercurrent immunoelectrophoresis, hemagglutinating (HA), and bactericidal (BC) antibody determinations were applied to seven patients with Haemophilus influenzae type B cellulitis. Capsular antigen was detected (greater than or equal to 10 ng/ml) in two patients at the time of admission and was found in two additional patients two days following hospitalization. Antibody responses in the patients with cellulitis were deficient, similar to those in children under 2 years of age with H influenzae type B meningitis. One of the patients with cellulitis had HA and BC antibody present in convalescent serum, and two additional patients demonstrated BC activity. The clinical presentations of our patients were typical except that violaceous discoloration of overlying skin was noted in only one of the seven. Two additional patients had atypical facial swelling that closely resembled angioneurotic edema and initially led to incorrect diagnosis. Countercurrent immunoelectrophoresis may  be helpful in the diagnosis of patients with Haemophilus cellulitis, particularly if serial serum determinations are performed. Bacterial cultures obtained from blood and aspirates of the lesion appear to be more sensitive in determining the cause of H influenzae type B cellulitis.", "contents": "Cellulitis due to Haemophilus influenzae type B. Antigenemia and antibody responses. The techniques of serum countercurrent immunoelectrophoresis, hemagglutinating (HA), and bactericidal (BC) antibody determinations were applied to seven patients with Haemophilus influenzae type B cellulitis. Capsular antigen was detected (greater than or equal to 10 ng/ml) in two patients at the time of admission and was found in two additional patients two days following hospitalization. Antibody responses in the patients with cellulitis were deficient, similar to those in children under 2 years of age with H influenzae type B meningitis. One of the patients with cellulitis had HA and BC antibody present in convalescent serum, and two additional patients demonstrated BC activity. The clinical presentations of our patients were typical except that violaceous discoloration of overlying skin was noted in only one of the seven. Two additional patients had atypical facial swelling that closely resembled angioneurotic edema and initially led to incorrect diagnosis. Countercurrent immunoelectrophoresis may  be helpful in the diagnosis of patients with Haemophilus cellulitis, particularly if serial serum determinations are performed. Bacterial cultures obtained from blood and aspirates of the lesion appear to be more sensitive in determining the cause of H influenzae type B cellulitis."}
{"id": "PMID:1086595", "title": "Congenital generalized fibromatosis. An African case with gingival hypertrophy and other unusual features.", "content": "What is believed to be the first reported case of congenital generalized fibromatosis in an African infant is described. Features in our patient, which were not noted in previous reports of the disease, include gingival hypertrophy, ankylosis of joints, skeletal hyperostosis, and lymphatic dilation of the ileal villi. Corticosteroid therapy was tried in the patient, but did not produce any beneficial effect.", "contents": "Congenital generalized fibromatosis. An African case with gingival hypertrophy and other unusual features. What is believed to be the first reported case of congenital generalized fibromatosis in an African infant is described. Features in our patient, which were not noted in previous reports of the disease, include gingival hypertrophy, ankylosis of joints, skeletal hyperostosis, and lymphatic dilation of the ileal villi. Corticosteroid therapy was tried in the patient, but did not produce any beneficial effect."}
{"id": "PMID:1086596", "title": "Histiocytic medullary reticulosis in acute lymphocytic leukemia of T cell origin.", "content": "A patient with acute lymphocytic leukemia of T (thymic-derived) cell origin was successfully treated and was maintained in remission for four months by combined chemotherapy. He died following a seven-week, fulminant course with fever, refractory pancytopenia, and marked hepatosplenomegaly. The autopsy showed lymphoid leukemic infiltration and extensive histiocytic medullary reticulosis in various organs. The possible relations between these two lymphoreticular diseases are discussed.", "contents": "Histiocytic medullary reticulosis in acute lymphocytic leukemia of T cell origin. A patient with acute lymphocytic leukemia of T (thymic-derived) cell origin was successfully treated and was maintained in remission for four months by combined chemotherapy. He died following a seven-week, fulminant course with fever, refractory pancytopenia, and marked hepatosplenomegaly. The autopsy showed lymphoid leukemic infiltration and extensive histiocytic medullary reticulosis in various organs. The possible relations between these two lymphoreticular diseases are discussed."}
{"id": "PMID:1086597", "title": "In vivo dissociation of factor VII (AHF) activity and factor VII-related antigen in von Willebrand's disease.", "content": "Using monospecific rabbit antihuman factor VIII antiserum, we have examined the amounts of factor VIII-related antigen and compared these to the levels of factor VIII procoagulant activity in normal subjects and patients with von Willebrand's disease. We have observed that even without transfusion all nine probands with von Willebrand's disease and 20 of their 34 relatives possessed a significantly elevated factor VIII activity/factor VIII-related antigen ratio when compared to that of 55 normal subjects. It is suggested that an elevated factor VIII activity/factor VIII-related antigen ratio may be used for detection of the carriers of von Willebrand's disease.", "contents": "In vivo dissociation of factor VII (AHF) activity and factor VII-related antigen in von Willebrand's disease. Using monospecific rabbit antihuman factor VIII antiserum, we have examined the amounts of factor VIII-related antigen and compared these to the levels of factor VIII procoagulant activity in normal subjects and patients with von Willebrand's disease. We have observed that even without transfusion all nine probands with von Willebrand's disease and 20 of their 34 relatives possessed a significantly elevated factor VIII activity/factor VIII-related antigen ratio when compared to that of 55 normal subjects. It is suggested that an elevated factor VIII activity/factor VIII-related antigen ratio may be used for detection of the carriers of von Willebrand's disease."}
{"id": "PMID:1086598", "title": "Need for \"counter-detailing\" antibiotics.", "content": "Selected antibiotic advertisements in medical journals are discussed to illustrate the misleading information that is often disseminated to physicians by the pharmaceutical industry. Laboratory and clinical data are presented to question the validity of selected advertisements which (1) encourage the use of Keflex for severe respiratory infections in children, (2) recommend the use of Keflex for the treatment of bacterial bronchitis, (3) suggest that high tissue penetration is a unique property of Vibramycin, (4) present pooled susceptability data which do not reflect microbial resistance patterns in the patient's hospital, (5) recommend twice-daily administration of Ancef for urinary tract infections but do not clearly state the potential danger of this regimen for other infections, (6) suggest that gentamicin should be given to adults in only two dosage sizes for the treatment of serious Gram-negative infections, and (7) lead the reader to assume that only women need to be treated for Trichomonas infections. It is suggested that as antibiotics are marketed, hospital therapeutics committees should evaluate their advantages and permit formulary additions for only those agents demonstrating increased efficacy, decreased toxicity or decreased cost. Pharmacists who monitor drug therapy can provide information to the physician which will increase his awareness of optimal antibiotic therapy.", "contents": "Need for \"counter-detailing\" antibiotics. Selected antibiotic advertisements in medical journals are discussed to illustrate the misleading information that is often disseminated to physicians by the pharmaceutical industry. Laboratory and clinical data are presented to question the validity of selected advertisements which (1) encourage the use of Keflex for severe respiratory infections in children, (2) recommend the use of Keflex for the treatment of bacterial bronchitis, (3) suggest that high tissue penetration is a unique property of Vibramycin, (4) present pooled susceptability data which do not reflect microbial resistance patterns in the patient's hospital, (5) recommend twice-daily administration of Ancef for urinary tract infections but do not clearly state the potential danger of this regimen for other infections, (6) suggest that gentamicin should be given to adults in only two dosage sizes for the treatment of serious Gram-negative infections, and (7) lead the reader to assume that only women need to be treated for Trichomonas infections. It is suggested that as antibiotics are marketed, hospital therapeutics committees should evaluate their advantages and permit formulary additions for only those agents demonstrating increased efficacy, decreased toxicity or decreased cost. Pharmacists who monitor drug therapy can provide information to the physician which will increase his awareness of optimal antibiotic therapy."}
{"id": "PMID:1086599", "title": "Vision profile of deaf children.", "content": "The vision status of 77 deaf children from Beverly School for the Deaf, Beverly, Massachusetts, was investigated in 6 general areas: visual acuity, refractive status, binocularity, color vision, pathology, and visual perceptual motor function. The significant results of this study that were different from the previously investigated work were in the areas of myopia and visual perceptual motor function. Myopia of only moderate to low degree was found in about 5.8% of the total poulation, which is significantly below the mean percentage found in a normal population. Almost 50% of the children showed deficiencies in and 1 or more of the 3 perceptual motor areas investiaged, i.e., visual memory, spatial organization, and Gesell copy forms.", "contents": "Vision profile of deaf children. The vision status of 77 deaf children from Beverly School for the Deaf, Beverly, Massachusetts, was investigated in 6 general areas: visual acuity, refractive status, binocularity, color vision, pathology, and visual perceptual motor function. The significant results of this study that were different from the previously investigated work were in the areas of myopia and visual perceptual motor function. Myopia of only moderate to low degree was found in about 5.8% of the total poulation, which is significantly below the mean percentage found in a normal population. Almost 50% of the children showed deficiencies in and 1 or more of the 3 perceptual motor areas investiaged, i.e., visual memory, spatial organization, and Gesell copy forms."}
{"id": "PMID:1086601", "title": "Mediastinal wound infection and aortocoronary graft patency.", "content": "In our experience, eight of ten aortocoronary grafts in five patients remained patent in the face of mediastinal infection. Combining reports of seventeen other grafts from the literature, we conclude that patency can be anticipated in approximately 70 per cent of such grafts and that mediastinal infection does not necessarily adversely affect aortocoronary saphenous vein bypass graft patency. We recommend aggressive therapy of mediastinal infection in this setting due to the high survival rate which can be anticipated with modern methods of therapy, as well as the high probability of graft patency.", "contents": "Mediastinal wound infection and aortocoronary graft patency. In our experience, eight of ten aortocoronary grafts in five patients remained patent in the face of mediastinal infection. Combining reports of seventeen other grafts from the literature, we conclude that patency can be anticipated in approximately 70 per cent of such grafts and that mediastinal infection does not necessarily adversely affect aortocoronary saphenous vein bypass graft patency. We recommend aggressive therapy of mediastinal infection in this setting due to the high survival rate which can be anticipated with modern methods of therapy, as well as the high probability of graft patency."}
{"id": "PMID:1086602", "title": "Fluorescein in the human optic disc. II. The fluorescein appearance rate.", "content": "Automatic intravenous infusion of fluorescein with constant speed during 25 sec is followed by a gradual increase of fluorescein in the retinal arteries and in the disc tissue. The slope of this increase Sd divided by the maximum fluorescein Md in an area of the disc Sd/Md is called Fd, the same for a retinal artery is called Fr. Fr was found to be independent of intraocular pressure in the range examined (mean pressures 15--30 mm applanantion). Fd/Fr = omega, the \"fluorescein appearance rate\" is correlated to the linear velocity of blood in the capillaries of the observed disc area and hence to its circulation. omega was found to be diminished in 6 out of 9 cases with ocular tension between 26 and 38 mm applanation, but not in 3 cases. It was not diminished in 4 cases with 22-25 mm applanation. Further studies are necessary to elucidate these findings suggesting some adaptation of disc circulation to intraocular pressure but not proving it: fluorescence from vessels behind the lamina cribrosa has the tendency to seemingly \"normalise\" omega.", "contents": "Fluorescein in the human optic disc. II. The fluorescein appearance rate. Automatic intravenous infusion of fluorescein with constant speed during 25 sec is followed by a gradual increase of fluorescein in the retinal arteries and in the disc tissue. The slope of this increase Sd divided by the maximum fluorescein Md in an area of the disc Sd/Md is called Fd, the same for a retinal artery is called Fr. Fr was found to be independent of intraocular pressure in the range examined (mean pressures 15--30 mm applanantion). Fd/Fr = omega, the \"fluorescein appearance rate\" is correlated to the linear velocity of blood in the capillaries of the observed disc area and hence to its circulation. omega was found to be diminished in 6 out of 9 cases with ocular tension between 26 and 38 mm applanation, but not in 3 cases. It was not diminished in 4 cases with 22-25 mm applanation. Further studies are necessary to elucidate these findings suggesting some adaptation of disc circulation to intraocular pressure but not proving it: fluorescence from vessels behind the lamina cribrosa has the tendency to seemingly \"normalise\" omega."}
{"id": "PMID:1086603", "title": "[Electrooculographic investigations after oculopression (author's transl)].", "content": "The influence of oculopression on the EOG in 6 persons with healthy eyes and in 7 patients with advanced glaucoma was investigated. The reaction of the potential produced by oculopression may be an objective feature of the ability of compensation.", "contents": "[Electrooculographic investigations after oculopression (author's transl)]. The influence of oculopression on the EOG in 6 persons with healthy eyes and in 7 patients with advanced glaucoma was investigated. The reaction of the potential produced by oculopression may be an objective feature of the ability of compensation."}
{"id": "PMID:1086604", "title": "Histopathology and ultrastructure of busulfan-induced cataract.", "content": "A 49-year-old man with chronic myelogenous leukemia developed dense posterior subcapsular cataracts and punctate cortical opacities after five years of busulfan (Myleran) therapy. We found that the histopathologic appearance of busulfan-induced cataracts is similar to other forms of human and experimental cataracts, regardless of cause. Ultrastructural examination revealed cortical liquefaction with formation of Morgagnian droplets, involving primarily the region from the equator to the posterior subcapsular space. In contrast to previous findings in rats, we observed posterior migration of equatorial nuclei and bladder cell formation. Also present were socalled crystalloid rays and abundant degenerate lens fiber membranes manifested as mutli-layered, irregularly folded configurations resembling myelin figures.", "contents": "Histopathology and ultrastructure of busulfan-induced cataract. A 49-year-old man with chronic myelogenous leukemia developed dense posterior subcapsular cataracts and punctate cortical opacities after five years of busulfan (Myleran) therapy. We found that the histopathologic appearance of busulfan-induced cataracts is similar to other forms of human and experimental cataracts, regardless of cause. Ultrastructural examination revealed cortical liquefaction with formation of Morgagnian droplets, involving primarily the region from the equator to the posterior subcapsular space. In contrast to previous findings in rats, we observed posterior migration of equatorial nuclei and bladder cell formation. Also present were socalled crystalloid rays and abundant degenerate lens fiber membranes manifested as mutli-layered, irregularly folded configurations resembling myelin figures."}
{"id": "PMID:1086605", "title": "Lens changes in car painters exposed to a mixture of organic solvents.", "content": "The relationship between exposure to organic solvents and the prevalence of lens changes was studied in a group of 92 car painters and a comparison group of 92 engineers from the Finnish State Railways. Both groups were examined ophthalmologically. Among the 92 car painters, with a mean age of 34.9 years (range of 21--64 years; S.D. 10.4 years), two had been operated on for a cataract and 46 had lens changes, mainly nuclear sclerosis, clefts, lamellar separation, or cortical and subcortical opacities not affecting visual acuity. In 69 age-matched pairs, for whom the factors inducing false-positive results had been excluded, 2 car painters had more lens changes than their age-matched pairs. Lens changes occurred with increased frequency after 10 years of exposure.", "contents": "Lens changes in car painters exposed to a mixture of organic solvents. The relationship between exposure to organic solvents and the prevalence of lens changes was studied in a group of 92 car painters and a comparison group of 92 engineers from the Finnish State Railways. Both groups were examined ophthalmologically. Among the 92 car painters, with a mean age of 34.9 years (range of 21--64 years; S.D. 10.4 years), two had been operated on for a cataract and 46 had lens changes, mainly nuclear sclerosis, clefts, lamellar separation, or cortical and subcortical opacities not affecting visual acuity. In 69 age-matched pairs, for whom the factors inducing false-positive results had been excluded, 2 car painters had more lens changes than their age-matched pairs. Lens changes occurred with increased frequency after 10 years of exposure."}
{"id": "PMID:1086606", "title": "Elevation of intraocular pressure after pars plana vitrectomy.", "content": "To determine the incidence and causes of increased intraocular pressure (IOP) after pars plana vitrectomy with the vitrophage, we studied 118 eyes under-going vitrectomy during a one-year period. Fifty-one (43%) were in diabetic patients; 67 (57%) were in nondiabetics. Of the entire group, 33 (28%) developed a postoperative increase in IOP, which was defined as a rise in IOP of at least 10 mm Hg above the preoperative level by applanation tonometry. Of the diabetic eyes 25 (49%) developed increased IOP; 11 (22%) of these eyes required surgical intervention for control of IOP. Of the nondiabetic eyes eight (12%) developed increased IOP postoperatively; all were either transiently elevated or medically controllable. Postoperative rubeosis iridis occurred only in diabetic eyes.", "contents": "Elevation of intraocular pressure after pars plana vitrectomy. To determine the incidence and causes of increased intraocular pressure (IOP) after pars plana vitrectomy with the vitrophage, we studied 118 eyes under-going vitrectomy during a one-year period. Fifty-one (43%) were in diabetic patients; 67 (57%) were in nondiabetics. Of the entire group, 33 (28%) developed a postoperative increase in IOP, which was defined as a rise in IOP of at least 10 mm Hg above the preoperative level by applanation tonometry. Of the diabetic eyes 25 (49%) developed increased IOP; 11 (22%) of these eyes required surgical intervention for control of IOP. Of the nondiabetic eyes eight (12%) developed increased IOP postoperatively; all were either transiently elevated or medically controllable. Postoperative rubeosis iridis occurred only in diabetic eyes."}
{"id": "PMID:1086607", "title": "Characteristics of human saccadic eye movements in different directions.", "content": "Saccadic human eye movements in different directions were recorded by an infrared technique. Disturbances by movements of the eyelids are prevented by means of a plaster pulling down the lower lid. The peak values of the velocity, acceleration, deceleration, and the duration of the saccades were drawn in a direction diagram. Each eye has its specific diagram. The shape of the diagram is independent of the saccadic amplitude. There are directions in which the values are equal for eccentric and centric saccades and those, in which significant differences are to be found. In the latter case centric saccades are faster than the eccentric ones.", "contents": "Characteristics of human saccadic eye movements in different directions. Saccadic human eye movements in different directions were recorded by an infrared technique. Disturbances by movements of the eyelids are prevented by means of a plaster pulling down the lower lid. The peak values of the velocity, acceleration, deceleration, and the duration of the saccades were drawn in a direction diagram. Each eye has its specific diagram. The shape of the diagram is independent of the saccadic amplitude. There are directions in which the values are equal for eccentric and centric saccades and those, in which significant differences are to be found. In the latter case centric saccades are faster than the eccentric ones."}
{"id": "PMID:1086608", "title": "[A canthi-skin electrode electroretinogram, independent of eye movements (author's transl)].", "content": "In order to avoid misinterpretation of ERG recordings taken with canthiskin electrodes, the potential deriving from the two electrodes was fed into a balancing amplifier that eliminated opposing voltages and amplified unidirectional voltages. During voluntary as well as nystagmus caused eye movements as well as those caused by nystagmus clearly distinguishable averaged ERG's were recorded.", "contents": "[A canthi-skin electrode electroretinogram, independent of eye movements (author's transl)]. In order to avoid misinterpretation of ERG recordings taken with canthiskin electrodes, the potential deriving from the two electrodes was fed into a balancing amplifier that eliminated opposing voltages and amplified unidirectional voltages. During voluntary as well as nystagmus caused eye movements as well as those caused by nystagmus clearly distinguishable averaged ERG's were recorded."}
{"id": "PMID:1086609", "title": "Diabetic retinopathy study. Preliminary results from 215 patients treated uniocularly with photocoagulation.", "content": "Preliminary results of a controlled study on diabetic retinopathy are reported. Two hundred and fifteen patients with proliferative (PDR) and nonproliferative (NPDR) retinopathy were uniocularly treated by Xenon photocoagulation and followed for a minimum of 6 months--6 years. Intra- and epiretinal new vessel formations (RNF) decreased in treated but increased in untreated eyes at a high statistical significance. A transition of background retinopathy into the proliferative stage was significantly retarded after photocoagulation. An improvement of new vessel formations on the disc (DNF) was not evident after 3 years of follow-up. The progression of preretinal neovascularization (PRNF) was significantly reduced only for 1--2 years after treatment. The incidence of massive vitreous hemorrhages, however, which showed the highest quotient of contingency with PRNF was much lower in treated eyes also after 3 years. The difference was significant only in extensively photocoagulated eyes with more than 100 lesions on average. The effect of photocoagulation on diabetic maculopathy with preservation of a useful visual acuity was highly significant only in diabetics under 60 years of age, the effect increasing with time as the control eyes fared worse. By contrast, in patients over 60 years of age, the course of maculpathy was not influenced, and a linear decrease of visual acuity occurred in both treated and untreated eyes.", "contents": "Diabetic retinopathy study. Preliminary results from 215 patients treated uniocularly with photocoagulation. Preliminary results of a controlled study on diabetic retinopathy are reported. Two hundred and fifteen patients with proliferative (PDR) and nonproliferative (NPDR) retinopathy were uniocularly treated by Xenon photocoagulation and followed for a minimum of 6 months--6 years. Intra- and epiretinal new vessel formations (RNF) decreased in treated but increased in untreated eyes at a high statistical significance. A transition of background retinopathy into the proliferative stage was significantly retarded after photocoagulation. An improvement of new vessel formations on the disc (DNF) was not evident after 3 years of follow-up. The progression of preretinal neovascularization (PRNF) was significantly reduced only for 1--2 years after treatment. The incidence of massive vitreous hemorrhages, however, which showed the highest quotient of contingency with PRNF was much lower in treated eyes also after 3 years. The difference was significant only in extensively photocoagulated eyes with more than 100 lesions on average. The effect of photocoagulation on diabetic maculopathy with preservation of a useful visual acuity was highly significant only in diabetics under 60 years of age, the effect increasing with time as the control eyes fared worse. By contrast, in patients over 60 years of age, the course of maculpathy was not influenced, and a linear decrease of visual acuity occurred in both treated and untreated eyes."}
{"id": "PMID:1086610", "title": "Regional ischemic infarcts of the retina.", "content": "Macroscopic and light microscopic features of regional ischemic infarcts of retina in autopsy eyes are described. Lesions were found throughout life span, most patients having significant primary or secondary vascular disease (younger had systemic hypertension, rheumatic heart disease, vasculitis or sickle hemoglobinopathy; most older patients had arteriosclerosis). Diabetes mellitus and infarction of other organs (including brain) also were common. Topographically almost all lesions were found in posterior fundus; most were temporal and involved anatomical macula. Microscopically there was destruction of inner retinal layers with preservation of outermost cells of inner nuclear layer; occasionally ganglion cell layer was relatively spared.", "contents": "Regional ischemic infarcts of the retina. Macroscopic and light microscopic features of regional ischemic infarcts of retina in autopsy eyes are described. Lesions were found throughout life span, most patients having significant primary or secondary vascular disease (younger had systemic hypertension, rheumatic heart disease, vasculitis or sickle hemoglobinopathy; most older patients had arteriosclerosis). Diabetes mellitus and infarction of other organs (including brain) also were common. Topographically almost all lesions were found in posterior fundus; most were temporal and involved anatomical macula. Microscopically there was destruction of inner retinal layers with preservation of outermost cells of inner nuclear layer; occasionally ganglion cell layer was relatively spared."}
{"id": "PMID:1086611", "title": "The effect of central chemical sympathectomy on the oxygen uptake; anaerobic glycolysis and lactic acid dehydrogenase activity in the retina of white rats.", "content": "Male Wistar rats were injected intraventricularly with two doses of 250 mcg of 6-hydroxydopamine (6-OHDA) in two consecutive days. Two weeks later the oxygen uptake, anaerobic glycolysis and lactic acid dehydrogenase (LDH) activity in the retina were determined. The decrease of oxygen uptake (-28%), anaerobic glycolysis (-31%) and LDH activity (-12%) in rats treated with 6-OHDA in comparison to control animals was found. The possible role of the adrenergic system in regulation of the metabolism of the retina is discussed.", "contents": "The effect of central chemical sympathectomy on the oxygen uptake; anaerobic glycolysis and lactic acid dehydrogenase activity in the retina of white rats. Male Wistar rats were injected intraventricularly with two doses of 250 mcg of 6-hydroxydopamine (6-OHDA) in two consecutive days. Two weeks later the oxygen uptake, anaerobic glycolysis and lactic acid dehydrogenase (LDH) activity in the retina were determined. The decrease of oxygen uptake (-28%), anaerobic glycolysis (-31%) and LDH activity (-12%) in rats treated with 6-OHDA in comparison to control animals was found. The possible role of the adrenergic system in regulation of the metabolism of the retina is discussed."}
{"id": "PMID:1086612", "title": "[The astigmatism bioblique. II. Mathematical description (author's transl)].", "content": "Optical surface showing an astigmatism bioblique could only approximately be described by mathematical formulas. The best approximation is that one which shows a close relation between regular and bioblique astigmatism. It allows the calculation of the best correcting cylinder, which brings the anavoidable residual astigmatism to a minimum.", "contents": "[The astigmatism bioblique. II. Mathematical description (author's transl)]. Optical surface showing an astigmatism bioblique could only approximately be described by mathematical formulas. The best approximation is that one which shows a close relation between regular and bioblique astigmatism. It allows the calculation of the best correcting cylinder, which brings the anavoidable residual astigmatism to a minimum."}
{"id": "PMID:1086613", "title": "The content of glycosaminoglycans in bovine corneal epithelium and precorneal fluid-film.", "content": "Histologic staining of hen cornea and precorneal fluid-film with ruthenium red lead to the assumption of existent coating glycosaminoglycans. Quantitative and chemical analytical examinations of bovine corneal epithelium with and without the fluid-film could not demonstrate the characteristic components of glycosaminoglycans. Even small amounts of glycosaminoglycans are not found after incubation of the cornea with radioactive precursors. But the extracted material have been proved by degradation with amyloglucosidase enzyme for pure glycogen. Most probably ruthenium red stains glycosaminoglycans nonspecifically but marks the glycoproteins as well which have been demonstrated in the precorneal fluid-film.", "contents": "The content of glycosaminoglycans in bovine corneal epithelium and precorneal fluid-film. Histologic staining of hen cornea and precorneal fluid-film with ruthenium red lead to the assumption of existent coating glycosaminoglycans. Quantitative and chemical analytical examinations of bovine corneal epithelium with and without the fluid-film could not demonstrate the characteristic components of glycosaminoglycans. Even small amounts of glycosaminoglycans are not found after incubation of the cornea with radioactive precursors. But the extracted material have been proved by degradation with amyloglucosidase enzyme for pure glycogen. Most probably ruthenium red stains glycosaminoglycans nonspecifically but marks the glycoproteins as well which have been demonstrated in the precorneal fluid-film."}
{"id": "PMID:1086614", "title": "Radiation therapy of choroidal metastases from breast cancer.", "content": "Radiation therapy of 26 patients with choroidal metastases from breast cancer has been reviewed. At the time of initial diagnosis of choroidal metastases 13 patients had bilateral choroidal metastases. The median age of the patients was 54 years and the survival from the first symptom of choroidal metastases varied from 2 to 45 months with a median survival of 9.5 months. End results of radiation therapy had been recorded in 22 patients. Local radiation therapy improved vision permanently in 16 patients.", "contents": "Radiation therapy of choroidal metastases from breast cancer. Radiation therapy of 26 patients with choroidal metastases from breast cancer has been reviewed. At the time of initial diagnosis of choroidal metastases 13 patients had bilateral choroidal metastases. The median age of the patients was 54 years and the survival from the first symptom of choroidal metastases varied from 2 to 45 months with a median survival of 9.5 months. End results of radiation therapy had been recorded in 22 patients. Local radiation therapy improved vision permanently in 16 patients."}
{"id": "PMID:1086615", "title": "Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury.", "content": "Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.", "contents": "Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury. Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed."}
{"id": "PMID:1086616", "title": "Effect of prostaglandin E2 on the isolated rabbit iris sphincter.", "content": "Experiments on twenty-eight New Zealand albino rabbits have shown that prostaglandin E2 causes contraction of the isolated iris sphincter and is about five times as effective as acetylcholine. Prostaglandin E2 does not act on the cholinergic receptors; the effect of prostaglandin E2 was not increased by eserine, and not abolished by atropine. In high doses indomethacin (3 X 10(-4) M) reversed the prostaglandin effect, but lower concentrations (3 X 10(-5) M) left it unaffected.", "contents": "Effect of prostaglandin E2 on the isolated rabbit iris sphincter. Experiments on twenty-eight New Zealand albino rabbits have shown that prostaglandin E2 causes contraction of the isolated iris sphincter and is about five times as effective as acetylcholine. Prostaglandin E2 does not act on the cholinergic receptors; the effect of prostaglandin E2 was not increased by eserine, and not abolished by atropine. In high doses indomethacin (3 X 10(-4) M) reversed the prostaglandin effect, but lower concentrations (3 X 10(-5) M) left it unaffected."}
{"id": "PMID:1086617", "title": "[Electromagnetic radiation damage to the retina (author's transl)].", "content": "Basic mechanism involved in electromagnetic radiation damage to the retina are described. Those are photochemical, thermal and non-linear effects. The absorption of radiant energy by the retina occurs primarily in the visible spectral range. Different wave lengths are absorbed in different layers of the retina. Photochemical injury for minimal lesions is uniform and the damaged area may be larger than the irradiated area. Thermal injury for minimal lesions produces usually a more intense central core of damage surrounded by edema. Non-linear effects are caused by ultrashort exposure times and are produced from strong electric fields, acoustic signals, shock waves, etc. generated by high temperature gradients. Wave lengths play a part in the severity and origin of retinal damage at relative long exposure times but contribute little to retinal injury at ultrashort exposures e.g. psec ranges.", "contents": "[Electromagnetic radiation damage to the retina (author's transl)]. Basic mechanism involved in electromagnetic radiation damage to the retina are described. Those are photochemical, thermal and non-linear effects. The absorption of radiant energy by the retina occurs primarily in the visible spectral range. Different wave lengths are absorbed in different layers of the retina. Photochemical injury for minimal lesions is uniform and the damaged area may be larger than the irradiated area. Thermal injury for minimal lesions produces usually a more intense central core of damage surrounded by edema. Non-linear effects are caused by ultrashort exposure times and are produced from strong electric fields, acoustic signals, shock waves, etc. generated by high temperature gradients. Wave lengths play a part in the severity and origin of retinal damage at relative long exposure times but contribute little to retinal injury at ultrashort exposures e.g. psec ranges."}
{"id": "PMID:1086618", "title": "Comparative electron-microscopic studies on the conus papillaris and its relationship to the retina in night and day active geckos.", "content": "The ultrastructure of the capillary endothelium of the conus papillaris within the vitreous body was studied in night, day, and mixed (night and day) active geckos (Homopholis wahlbergi, Gekko gecko, Pachydactylus bibronii, Tarentola mauritanica, Lygodactylus conraui, Phelsuma guimbeaui, Phelsuma madagascariensis). Capillary endothelial cells were poorly developed in night and mixed active animals, whereas in day active animals they were highly organized. In particular the number and height of the luminal and basal microvilli and the frequency of micropinocytotic vesicles were distinctly increased in day active geckos. It is assumed that these structural mechanisms improve the transendothelial transportation capacity of metabolic substances. When the thickness of the retinal layers was compared, we found that the inner retinal layers of those geckos in which the conus capillaries were poorly developed, were approximately 60% of the thickness of the inner retina in the day active geckos. The results indicate that the structural organization of conal endothelial cells is related primarily to the retinal structure rather than to the animals' daytime behavior. Furthermore, our observations support the theory that the conus papillaris of lizards, like the pecten oculi of birds, has a primary function in the nutrition of the avascular retina and/or is involved in the exchange of fluid in the vitreous.", "contents": "Comparative electron-microscopic studies on the conus papillaris and its relationship to the retina in night and day active geckos. The ultrastructure of the capillary endothelium of the conus papillaris within the vitreous body was studied in night, day, and mixed (night and day) active geckos (Homopholis wahlbergi, Gekko gecko, Pachydactylus bibronii, Tarentola mauritanica, Lygodactylus conraui, Phelsuma guimbeaui, Phelsuma madagascariensis). Capillary endothelial cells were poorly developed in night and mixed active animals, whereas in day active animals they were highly organized. In particular the number and height of the luminal and basal microvilli and the frequency of micropinocytotic vesicles were distinctly increased in day active geckos. It is assumed that these structural mechanisms improve the transendothelial transportation capacity of metabolic substances. When the thickness of the retinal layers was compared, we found that the inner retinal layers of those geckos in which the conus capillaries were poorly developed, were approximately 60% of the thickness of the inner retina in the day active geckos. The results indicate that the structural organization of conal endothelial cells is related primarily to the retinal structure rather than to the animals' daytime behavior. Furthermore, our observations support the theory that the conus papillaris of lizards, like the pecten oculi of birds, has a primary function in the nutrition of the avascular retina and/or is involved in the exchange of fluid in the vitreous."}
{"id": "PMID:1086619", "title": "Influence of sodium chloride on the amount of CRF-granules and \"classical\" neurosecretory material (NSM) in the neurohypophysis of bilaterally adrenalectomized rats.", "content": "Bilaterally adrenalectomized female SPF Wistar rats were given tap water containing various concentrations of sodium chloride to drink ad lib. After a survival period of 14 days the animals were killed by perfusion-fixation. Their brains were removed, fixed in picric acid formalin solution and embedded in Paraplast. Serial sections of median eminence and hypophysial posterior lobe were cut and stained with crotonaldehyde-fuchsine. In the stained sections the amount of CRF-granules visible in the external zone of the median eminence, and the amount of \"classical\" neurosecretory material (NSM) occuring in the internal zone of the median eminence and in the posterior lobe of the hypophysis were estimated. After administration of drinking solutions to which 0%-1% sodium chloride were added, the amount of CRF-gr anules increased with increasing NaCL uptake, whereas the amount of \"classical\" NSM was not changed. Application of tap water containing 2% NaCl led to a marked decrease in the amount of \"classical\" NSM but did only slightly impair the amount of CRF-granules. From the findings it is concluded that 1. following bilateral adrenalectomy administration of sodium chloride inhibits the secretion of CRF-granules; 2. CRF-granules and \"classical\" NSM, although histochemical identical, have different functional significances; 3. the mechanisms regulating the secretion of CRF-granules are different from those controlling the release of \"classical\" NSM.", "contents": "Influence of sodium chloride on the amount of CRF-granules and \"classical\" neurosecretory material (NSM) in the neurohypophysis of bilaterally adrenalectomized rats. Bilaterally adrenalectomized female SPF Wistar rats were given tap water containing various concentrations of sodium chloride to drink ad lib. After a survival period of 14 days the animals were killed by perfusion-fixation. Their brains were removed, fixed in picric acid formalin solution and embedded in Paraplast. Serial sections of median eminence and hypophysial posterior lobe were cut and stained with crotonaldehyde-fuchsine. In the stained sections the amount of CRF-granules visible in the external zone of the median eminence, and the amount of \"classical\" neurosecretory material (NSM) occuring in the internal zone of the median eminence and in the posterior lobe of the hypophysis were estimated. After administration of drinking solutions to which 0%-1% sodium chloride were added, the amount of CRF-gr anules increased with increasing NaCL uptake, whereas the amount of \"classical\" NSM was not changed. Application of tap water containing 2% NaCl led to a marked decrease in the amount of \"classical\" NSM but did only slightly impair the amount of CRF-granules. From the findings it is concluded that 1. following bilateral adrenalectomy administration of sodium chloride inhibits the secretion of CRF-granules; 2. CRF-granules and \"classical\" NSM, although histochemical identical, have different functional significances; 3. the mechanisms regulating the secretion of CRF-granules are different from those controlling the release of \"classical\" NSM."}
{"id": "PMID:1086621", "title": "Follow-up evaluation of a prevalence study for chronic bronchitis and chronic airway obstruction.", "content": "Follow-up of the prevalence study of respiratory symptoms and chronic airway obstruction was performed after a 6- to 7-year interval. One hundred fifteen of 117 subjects (98%) originally labeled \"abnormal\" (chronic bronchitis or asthma by history, or ratio of 1-sec forced expiratory volume to forced vital capacity less than 60 per cent), and 111 of a random sample of 116 subjects (96%) originally labeled \"normal\" were traced. Nineteen patients were dead, 14 \"abnormal\" subjects, and 5 \"normal\" subjects (P less than 0.01). Eighty-eight of the originally labeled \"abnormal\" subjects and 91 of the random \"normal\" sample could be retested by spirometry. Subjects with lower initial ratios of 1-sec forced expiratory volume to forced vital capacity tended to lose more 1-sec forced expiratory volume and forced vital capacity. Early identification of respiratory symptoms and spirometric abnormalities may identify persons at increased risk of death who could benefit from appropriate therapy.", "contents": "Follow-up evaluation of a prevalence study for chronic bronchitis and chronic airway obstruction. Follow-up of the prevalence study of respiratory symptoms and chronic airway obstruction was performed after a 6- to 7-year interval. One hundred fifteen of 117 subjects (98%) originally labeled \"abnormal\" (chronic bronchitis or asthma by history, or ratio of 1-sec forced expiratory volume to forced vital capacity less than 60 per cent), and 111 of a random sample of 116 subjects (96%) originally labeled \"normal\" were traced. Nineteen patients were dead, 14 \"abnormal\" subjects, and 5 \"normal\" subjects (P less than 0.01). Eighty-eight of the originally labeled \"abnormal\" subjects and 91 of the random \"normal\" sample could be retested by spirometry. Subjects with lower initial ratios of 1-sec forced expiratory volume to forced vital capacity tended to lose more 1-sec forced expiratory volume and forced vital capacity. Early identification of respiratory symptoms and spirometric abnormalities may identify persons at increased risk of death who could benefit from appropriate therapy."}
{"id": "PMID:1086622", "title": "Clinical, epidemiologic, and pulmonary function studies in alpha,-antitrypsin-deficient subjects of Pi Z type.", "content": "The results of pulmonary function testing and systematic medical history and epidemiologic data collection are reported for 20 persons with alpha 1-antitrypsin deficiency of Pi Z phenotype. The most common symptom, reported in 19 subjects (95 per cent), was dyspnea on exertion; 16 subjects (80 per cent) gave a history of wheezing, and 8 (40 percent) reported chronic cough and sputum production. The 8 women who had been pregnant reported a miscarriage rate of 29 per cent for all pregnancies. Respiratory symptoms and disease were commonly reported in the children of study subjects. Pulmonary function testing revealed abnormalities for 18 of 20 subjects, all of those 26 or more years of age. The test that was most frequently abnormal was the 1-sec forced expiratory volume expressed as a per cent of the forced vital capacity. All pulmonary function studies demonstrated a trend toward increased impairment with increased age, which was evident by the fourth decade. Within this group of persons having severe alpha1-antitrypsin deficiency, there was no correlation between serum concentrations of antitrypsin and subjective or objective indices of pulmonary disease. A group of 7 subjects who were incidentally found to have Pi Z alpha1-antitrypsin deficiency exhibited symptoms and pulmonary function abnormalities comparable to those of 13 subjects who were originally referred for known or suspected pulmonary disease. These data suggest that if interventions such as smoking cessation and occupational counseling are to be effective, they should be initiated before the fourth decade of life.", "contents": "Clinical, epidemiologic, and pulmonary function studies in alpha,-antitrypsin-deficient subjects of Pi Z type. The results of pulmonary function testing and systematic medical history and epidemiologic data collection are reported for 20 persons with alpha 1-antitrypsin deficiency of Pi Z phenotype. The most common symptom, reported in 19 subjects (95 per cent), was dyspnea on exertion; 16 subjects (80 per cent) gave a history of wheezing, and 8 (40 percent) reported chronic cough and sputum production. The 8 women who had been pregnant reported a miscarriage rate of 29 per cent for all pregnancies. Respiratory symptoms and disease were commonly reported in the children of study subjects. Pulmonary function testing revealed abnormalities for 18 of 20 subjects, all of those 26 or more years of age. The test that was most frequently abnormal was the 1-sec forced expiratory volume expressed as a per cent of the forced vital capacity. All pulmonary function studies demonstrated a trend toward increased impairment with increased age, which was evident by the fourth decade. Within this group of persons having severe alpha1-antitrypsin deficiency, there was no correlation between serum concentrations of antitrypsin and subjective or objective indices of pulmonary disease. A group of 7 subjects who were incidentally found to have Pi Z alpha1-antitrypsin deficiency exhibited symptoms and pulmonary function abnormalities comparable to those of 13 subjects who were originally referred for known or suspected pulmonary disease. These data suggest that if interventions such as smoking cessation and occupational counseling are to be effective, they should be initiated before the fourth decade of life."}
{"id": "PMID:1086623", "title": "Lymphocyte subpopulations in sarcoidosis: correlation with disease aetivity and duration.", "content": "We studied lymphocytes from the peripheral blood of 29 sarcoid patients to relate T- and B-cell populations with disease activity and duration. In patients with acute (less than 1 year) and chronic (greater than or equal to 9 years) active disease, the absolute lymphocyte count was reduced; the absolute number of T cells was reduced; and the proliferative response of lymphocytes to phytohemagglutinin was depressed compared with control subjects. Ten of 21 patients with active disease had 5% to 32% atypical lymphocytes. The proportion of cells bearing surface immunoglobulin (Ig) was increased in patients with active disease, but the absolute number of cells bearing surface Ig did not differ significantly from controls. However, studies using overnight in-vitro culture indicated that a large fraction of these cells bound exogenous Ig. The number of cells indentified by complement receptors was significantly reduced in patients with active disease. In contrast, patients who had complete reasolution of their disease showed no significant differences from controls in either T- or B-cell populations or in the proliferative response of their lymphocytes to phytohemagglutinin.", "contents": "Lymphocyte subpopulations in sarcoidosis: correlation with disease aetivity and duration. We studied lymphocytes from the peripheral blood of 29 sarcoid patients to relate T- and B-cell populations with disease activity and duration. In patients with acute (less than 1 year) and chronic (greater than or equal to 9 years) active disease, the absolute lymphocyte count was reduced; the absolute number of T cells was reduced; and the proliferative response of lymphocytes to phytohemagglutinin was depressed compared with control subjects. Ten of 21 patients with active disease had 5% to 32% atypical lymphocytes. The proportion of cells bearing surface immunoglobulin (Ig) was increased in patients with active disease, but the absolute number of cells bearing surface Ig did not differ significantly from controls. However, studies using overnight in-vitro culture indicated that a large fraction of these cells bound exogenous Ig. The number of cells indentified by complement receptors was significantly reduced in patients with active disease. In contrast, patients who had complete reasolution of their disease showed no significant differences from controls in either T- or B-cell populations or in the proliferative response of their lymphocytes to phytohemagglutinin."}
{"id": "PMID:1086624", "title": "Late infections following filtering procedures.", "content": "In 364 patients with filtering conjunctival blebs, the incidence and outcome of exogenous late infections were determined. The rate was lowest among patients who had undergone Scheie's procedure. After treatment, the visual acuity in 62.5% of the infected cases was the same as before the infection occurred.", "contents": "Late infections following filtering procedures. In 364 patients with filtering conjunctival blebs, the incidence and outcome of exogenous late infections were determined. The rate was lowest among patients who had undergone Scheie's procedure. After treatment, the visual acuity in 62.5% of the infected cases was the same as before the infection occurred."}
{"id": "PMID:1086626", "title": "[Autoradiographic study of the DNA synthesis process along metaphasic chromosomes of Pleurodeles waltlii Michah (urodele amphibian)].", "content": "Chromosomes from embryos (stage 34) of the amphibian urodele Pleurodeles waltlii were labeled by 3H-thymidine at different times of the DNA synthesis cycle and studied at metaphase. Terminal segments of the chromosomes, induced secondary constrictions, and satellites were found to be late-labeling. Therefore, they may be considered as formed at least partially by heterochromatin. However, after other treatments or stainings, they do not present other features considered characteristic of heterochromatin. The question of the heterogeneity of their structures is discussed and the ambiguity of the term heterochromatin is emphasized. The proximal part of the long arm of chromosome VII shows characteristics similar to satellites, which may indicate the presence of a previously unrecognized satellite.", "contents": "[Autoradiographic study of the DNA synthesis process along metaphasic chromosomes of Pleurodeles waltlii Michah (urodele amphibian)]. Chromosomes from embryos (stage 34) of the amphibian urodele Pleurodeles waltlii were labeled by 3H-thymidine at different times of the DNA synthesis cycle and studied at metaphase. Terminal segments of the chromosomes, induced secondary constrictions, and satellites were found to be late-labeling. Therefore, they may be considered as formed at least partially by heterochromatin. However, after other treatments or stainings, they do not present other features considered characteristic of heterochromatin. The question of the heterogeneity of their structures is discussed and the ambiguity of the term heterochromatin is emphasized. The proximal part of the long arm of chromosome VII shows characteristics similar to satellites, which may indicate the presence of a previously unrecognized satellite."}
{"id": "PMID:1086627", "title": "[Holoprosencephaly by triploidy 69 XXX in a 5 month old fetus].", "content": "Microcephaly, occipital meningocele, and uveal coloboma were observed in a 5-month-old fetus with a 69, XXX karyotype. Autopsy showed an holoprosencephaly, which has never before been reported in triploidy.", "contents": "[Holoprosencephaly by triploidy 69 XXX in a 5 month old fetus]. Microcephaly, occipital meningocele, and uveal coloboma were observed in a 5-month-old fetus with a 69, XXX karyotype. Autopsy showed an holoprosencephaly, which has never before been reported in triploidy."}
{"id": "PMID:1086628", "title": "[2 cases of partial trisomy 10p due to a paternal translocation t(10p;18)(p13;q23)].", "content": "Two brothers trisomic for the distal two thirds of 10p are reported. Trisomy results from the malsegregation of a familial translocation rcp (10;18)(p13;q23) present in the father, a half-brother and the grand-father of the propositi. The phenotype is comparable to that of other 10p trisomic patients reported in the literature.", "contents": "[2 cases of partial trisomy 10p due to a paternal translocation t(10p;18)(p13;q23)]. Two brothers trisomic for the distal two thirds of 10p are reported. Trisomy results from the malsegregation of a familial translocation rcp (10;18)(p13;q23) present in the father, a half-brother and the grand-father of the propositi. The phenotype is comparable to that of other 10p trisomic patients reported in the literature."}
{"id": "PMID:1086629", "title": "[45, X/47, XY, +13 mosaicism in a 19-year-old girl].", "content": "The 45, X/47, XY, +13 mosaicism was observed in a 19-year-old mentally deficient girl who was examined because of primary amenorrhea. Certain clinical features were reminiscent of Turner's syndrome, while no features of trisomy 13 were present. The study of blood groups, HLA genotypes, and cytogenetic markers provided no evidence of chimerism.", "contents": "[45, X/47, XY, +13 mosaicism in a 19-year-old girl]. The 45, X/47, XY, +13 mosaicism was observed in a 19-year-old mentally deficient girl who was examined because of primary amenorrhea. Certain clinical features were reminiscent of Turner's syndrome, while no features of trisomy 13 were present. The study of blood groups, HLA genotypes, and cytogenetic markers provided no evidence of chimerism."}
{"id": "PMID:1086630", "title": "[Chromosome effect of cyclophosphamide in various strains of mice].", "content": "The chromosomes of four different mouse strains were examined after administration of a single dose of cyclophosphamide, 100 mg/kg. Chromosomal breaks and rearrangements were produced in C3H mice three to four times as frequently and in AKR mice twice as frequently as in C57 B1 and XLII Orl mice. This indicates that cytogenetic investigations in mutation research using mice as in vivo test systems may be variable depending on the mouse strain employed. Because the effect of the alkylating agent was not pronounced in the two strains having a high incidence of cancer and leukemia (C3H and AKR), the possibility of a relationship between chromosomal instability and cancer proneness is discussed.", "contents": "[Chromosome effect of cyclophosphamide in various strains of mice]. The chromosomes of four different mouse strains were examined after administration of a single dose of cyclophosphamide, 100 mg/kg. Chromosomal breaks and rearrangements were produced in C3H mice three to four times as frequently and in AKR mice twice as frequently as in C57 B1 and XLII Orl mice. This indicates that cytogenetic investigations in mutation research using mice as in vivo test systems may be variable depending on the mouse strain employed. Because the effect of the alkylating agent was not pronounced in the two strains having a high incidence of cancer and leukemia (C3H and AKR), the possibility of a relationship between chromosomal instability and cancer proneness is discussed."}
{"id": "PMID:1086631", "title": "[Translocation t(Y;14) in an azoospermic man].", "content": "A study of meiosis in an azoospermic man with a translocation between the Y and 14 chromosomes shows complete arrest of gametogenesis after the second division (spermatocyte II stage). At pachytene, the distal segment of the Y chromosome which is translocated onto the 14, is in contact with the sex vesicle.", "contents": "[Translocation t(Y;14) in an azoospermic man]. A study of meiosis in an azoospermic man with a translocation between the Y and 14 chromosomes shows complete arrest of gametogenesis after the second division (spermatocyte II stage). At pachytene, the distal segment of the Y chromosome which is translocated onto the 14, is in contact with the sex vesicle."}
{"id": "PMID:1086632", "title": "[Translocation 46,X, t(Y;7)(q122;q11) in a case of male sterility].", "content": "A Y-autosome translocation--46,X,t(Y;7)(q122;q111)--in a steril man is reported and compared with others already reported in the literature.", "contents": "[Translocation 46,X, t(Y;7)(q122;q11) in a case of male sterility]. A Y-autosome translocation--46,X,t(Y;7)(q122;q111)--in a steril man is reported and compared with others already reported in the literature."}
{"id": "PMID:1086634", "title": "Susceptibility of Haemophilus influenzae type b to cefatrizine, ampicillin, and chloramphenicol.", "content": "The susceptibility of 269 isolates of Haemophilus influenzae type b to cefatrizine (BL-S640), ampicillin, and chloramphenicol was evaluated by disk diffusion susceptibility tests, using a modified Bauer-Kirby method. Broth dilution susceptibility tests were performed on 88 of these isolates, including all isolates resistant by disk to cefatrizine or ampicillin. Six of the isolates were resistant by disk to cefatrizine (zone size, <16 mm), four were resistant to ampicillin (zone size, <19 mm), and none were resistant to chloramphenicol (zone size, <17 mm). Only two of the six isolates of H. influenzae that were resistant to cefatrizine by disk were resistant to more than 4 mug of drug per ml. The four organisms resistant to ampicillin on disk were resistant, when tested by the broth method, to >128 mug/ml. These four ampicillin-resistant H. influenzae were susceptible to <4 mug of cefatrizine per ml. The two isolates resistant to >4 mug of cefatrizine per ml were susceptible to 0.5 and 2 mug of ampicillin, respectively, per ml. The activity of cefatrizine appears to be comparable in vitro to ampicillin against H. influenzae.", "contents": "Susceptibility of Haemophilus influenzae type b to cefatrizine, ampicillin, and chloramphenicol. The susceptibility of 269 isolates of Haemophilus influenzae type b to cefatrizine (BL-S640), ampicillin, and chloramphenicol was evaluated by disk diffusion susceptibility tests, using a modified Bauer-Kirby method. Broth dilution susceptibility tests were performed on 88 of these isolates, including all isolates resistant by disk to cefatrizine or ampicillin. Six of the isolates were resistant by disk to cefatrizine (zone size, <16 mm), four were resistant to ampicillin (zone size, <19 mm), and none were resistant to chloramphenicol (zone size, <17 mm). Only two of the six isolates of H. influenzae that were resistant to cefatrizine by disk were resistant to more than 4 mug of drug per ml. The four organisms resistant to ampicillin on disk were resistant, when tested by the broth method, to >128 mug/ml. These four ampicillin-resistant H. influenzae were susceptible to <4 mug of cefatrizine per ml. The two isolates resistant to >4 mug of cefatrizine per ml were susceptible to 0.5 and 2 mug of ampicillin, respectively, per ml. The activity of cefatrizine appears to be comparable in vitro to ampicillin against H. influenzae."}
{"id": "PMID:1086635", "title": "New method for detecting in vitro inactivation of penicillins by Haemophilus influenzae and Staphlycoccus aureus.", "content": "A new technique for detecting penicillinase production in Haemophilus influenzae and Staphylococcus aureus was compared with the capillary procedure for detecting beta-lactamase and the Bauer-Kirby disk susceptibility procedure. Isolates were classified similarly by all three procedures.", "contents": "New method for detecting in vitro inactivation of penicillins by Haemophilus influenzae and Staphlycoccus aureus. A new technique for detecting penicillinase production in Haemophilus influenzae and Staphylococcus aureus was compared with the capillary procedure for detecting beta-lactamase and the Bauer-Kirby disk susceptibility procedure. Isolates were classified similarly by all three procedures."}
{"id": "PMID:1086636", "title": "Chemotaxis and transport of amino acids in Allomyces arbuscula.", "content": "Among a number of amino acids tested, L-lysine and L-arginine are the principal attractants in the chemotaxis of the zygotes of Allomyces arbuscula. The reaction can be stimulated to a greater or lesser extent by a number of compounds chemically related to L-leucine. No relationship between transport of attracting amino acids and their effect on chemotaxis has been found.", "contents": "Chemotaxis and transport of amino acids in Allomyces arbuscula. Among a number of amino acids tested, L-lysine and L-arginine are the principal attractants in the chemotaxis of the zygotes of Allomyces arbuscula. The reaction can be stimulated to a greater or lesser extent by a number of compounds chemically related to L-leucine. No relationship between transport of attracting amino acids and their effect on chemotaxis has been found."}
{"id": "PMID:1086637", "title": "Formation of protoplasts from Ustilago maydis.", "content": "Protoplasts of Ustilago maydis were obtained by incubating sporidia of the fungus with a combination of Helicase and a commercial \"Onozuka\" R-10 enzyme preparation of Trichoderma harzianum in the presence of 0.6 M (NH4)2 SO4 as an osmotic stabilizer. In the presence of the organic stabilizers sorbitol and sucrose, however, the release of protoplasts was inhibited. Combinations of Helicase with other lytic enzymes such as cellulase from Aspergillus niger, cellulase and hemicellulase from Rhizopus, and Driselase or Nagarse were inactive.", "contents": "Formation of protoplasts from Ustilago maydis. Protoplasts of Ustilago maydis were obtained by incubating sporidia of the fungus with a combination of Helicase and a commercial \"Onozuka\" R-10 enzyme preparation of Trichoderma harzianum in the presence of 0.6 M (NH4)2 SO4 as an osmotic stabilizer. In the presence of the organic stabilizers sorbitol and sucrose, however, the release of protoplasts was inhibited. Combinations of Helicase with other lytic enzymes such as cellulase from Aspergillus niger, cellulase and hemicellulase from Rhizopus, and Driselase or Nagarse were inactive."}
{"id": "PMID:1086633", "title": "Subpopulations of B lymphocytes and the carriage of immunological memory.", "content": "Thoracic duct lymphocytes from rats primed with DNP-BGG confer on irradiated, syngeneic recipients the ability to mount a large, IgG anti-DNP response after challenge with the immunizing conjugate. An analysis of this adoptive response lead to the following conclusions. the B memory cells which generate the IgG response carry IgG on their surface and are present in a subpopulation amounting to less than l0% of all B cells in thoracic duct lymph. The view that memory cells carry exclusively IgD is untenable. The majority of B lymphocytes in thoracic duct lymph are recirculating cells which carry surface IgM...", "contents": "Subpopulations of B lymphocytes and the carriage of immunological memory. Thoracic duct lymphocytes from rats primed with DNP-BGG confer on irradiated, syngeneic recipients the ability to mount a large, IgG anti-DNP response after challenge with the immunizing conjugate. An analysis of this adoptive response lead to the following conclusions. the B memory cells which generate the IgG response carry IgG on their surface and are present in a subpopulation amounting to less than l0% of all B cells in thoracic duct lymph. The view that memory cells carry exclusively IgD is untenable. The majority of B lymphocytes in thoracic duct lymph are recirculating cells which carry surface IgM..."}
{"id": "PMID:1086638", "title": "Lactate metabolism in Propionibacterium pentosaceum growing with nitrate or oxygen as hydrogen acceptor.", "content": "When anaerobic cultures of Propionibacterium pentosaceum were shifted to low dissolved-oxygen concentration (D.O.C.), acetate production from lactate diminished and propionate production stopped, whereas pyruvate accumulated and oxygen was consumed. Assuming that energy is generated in the electron transfer to oxygen, YATP values (g dry wt bacteria/mole ATP) of between 7.2 and 11.9 were calculated from molar growth yields and product formation. When oxidative phosphorylation in the electron transfer to oxygen was ignored, unreasonably high YATP values were obtained. From these results it is concluded that energy is indeed generated in the electron transfer to oxygen. However, synthesis of cytochrome b was strongly repressed by oxygen. Furthermore, synthesis of all catabolic enzymes studied was impaired in bacteria growing at low D.O.C. Thus, the anaerobic character of P. pentosaceum may be explained by the inhibition of synthesis of both cytochrome b and enzymes in the presence of oxygen. It was demonstrated that nitrate reductase is synthesized constitutively in P. pentosaceum. Synthesis of nitrate reductase was stimulated by nitrate and repressed by oxygen. Synthesis of fumarate reductase was also repressed by oxygen, whereas only a small effect of nitrate on this enzyme was observed. However, propionate formation is inhibited during growth with nitrate. The absence of propionate formation in the presence of oxygen and nitrate is explained by inavailability of NADH needed for the conversion of oxaloacetate into malate in the reductive pathway to succinate, so that succinate and propionate cannot be formed.", "contents": "Lactate metabolism in Propionibacterium pentosaceum growing with nitrate or oxygen as hydrogen acceptor. When anaerobic cultures of Propionibacterium pentosaceum were shifted to low dissolved-oxygen concentration (D.O.C.), acetate production from lactate diminished and propionate production stopped, whereas pyruvate accumulated and oxygen was consumed. Assuming that energy is generated in the electron transfer to oxygen, YATP values (g dry wt bacteria/mole ATP) of between 7.2 and 11.9 were calculated from molar growth yields and product formation. When oxidative phosphorylation in the electron transfer to oxygen was ignored, unreasonably high YATP values were obtained. From these results it is concluded that energy is indeed generated in the electron transfer to oxygen. However, synthesis of cytochrome b was strongly repressed by oxygen. Furthermore, synthesis of all catabolic enzymes studied was impaired in bacteria growing at low D.O.C. Thus, the anaerobic character of P. pentosaceum may be explained by the inhibition of synthesis of both cytochrome b and enzymes in the presence of oxygen. It was demonstrated that nitrate reductase is synthesized constitutively in P. pentosaceum. Synthesis of nitrate reductase was stimulated by nitrate and repressed by oxygen. Synthesis of fumarate reductase was also repressed by oxygen, whereas only a small effect of nitrate on this enzyme was observed. However, propionate formation is inhibited during growth with nitrate. The absence of propionate formation in the presence of oxygen and nitrate is explained by inavailability of NADH needed for the conversion of oxaloacetate into malate in the reductive pathway to succinate, so that succinate and propionate cannot be formed."}
{"id": "PMID:1086639", "title": "Starch degradation by the mould Trichoderma viride. II. Regulation of enzyme synthesis.", "content": "The synthesis of amylolytic enzymes by the maltose not-utilizing Trichoderma viride strain CBS 354.44 requires the presence of starch or dextrins. Several readily utilizable carbon sources such as glucose and glutamic acid were shown to exert a strong catabolite repression which completely inhibited enzyme induction by starch or dextrins. Enzyme synthesis occurs in the exponential and in the stationary growth phase. In the latter, the ratio between saccharifying and dextrinizing enzyme activity is invariably high. In the exponential growth phase this ratio depends on the nature of the inducing substrate. Growth on starch results in an initially high production of dextrinizing activity, the saccharifying one becoming predominant in the course of exponential growth. The latter activity in dextrin DE 30 cultures is predominant from the very beginning. Thus, the amylolytic enzyme system of T. viride consists of at least two different enzymes, the synthesis of each being controlled specifically. The careful regulation of the synthesis of the dextrinizing enzyme is discussed with special reference to the production of non-utilizable maltose by the latter.", "contents": "Starch degradation by the mould Trichoderma viride. II. Regulation of enzyme synthesis. The synthesis of amylolytic enzymes by the maltose not-utilizing Trichoderma viride strain CBS 354.44 requires the presence of starch or dextrins. Several readily utilizable carbon sources such as glucose and glutamic acid were shown to exert a strong catabolite repression which completely inhibited enzyme induction by starch or dextrins. Enzyme synthesis occurs in the exponential and in the stationary growth phase. In the latter, the ratio between saccharifying and dextrinizing enzyme activity is invariably high. In the exponential growth phase this ratio depends on the nature of the inducing substrate. Growth on starch results in an initially high production of dextrinizing activity, the saccharifying one becoming predominant in the course of exponential growth. The latter activity in dextrin DE 30 cultures is predominant from the very beginning. Thus, the amylolytic enzyme system of T. viride consists of at least two different enzymes, the synthesis of each being controlled specifically. The careful regulation of the synthesis of the dextrinizing enzyme is discussed with special reference to the production of non-utilizable maltose by the latter."}
{"id": "PMID:1086640", "title": "Microbial metabolism of alkylbenzene sulphonates. Variance in enzyme complement of a Bacillus in response to growth substrate.", "content": "Growth of a Bacillus species at the expense of an alkylbenzene sulphonate (ABS) synthetic detergent homologue (1-phenylundecane-p-sulphonate, 11-ABS) containing an odd number of carbon atoms in the alkyl side chain induced an enzyme complement able to biodegrade 11-ABS by alkyl side-chain oxidation, and \"ortho cleavage\" aromatic-ring oxidation reactions. Growth of the Bacillus at the expense of an ABS homologue containing an even number of carbon atoms in the alkyl side chain (1-phenyldodecane-p-sulphonate, 12-ABS) induced an enzyme complement able to biodegrade 12-ABS by alkyl side-chain oxidation, and \"meta cleavage\" aromatic-ring oxidation reactions. The results of a number of different growth and enzyme induction experiments confirm that both 11-ABS and 12-ABS are initially biodegraded by an identical complement of enzymes catalysing the alkyl-side-chain oxidation reactions, but that the subsequent metabolism of the aromatic moieties remaining after the removal of the alkyl side chain from 11-ABS and 12-ABS occurs by two separate pathways requiring the de novo induction of different substrate-specific enzyme complements. The detection of the predicted changes in enzyme complement subsequent to changes in the growth substrate of the Bacillus provide confirmation of the biodegradation pathways operating in the microorganism.", "contents": "Microbial metabolism of alkylbenzene sulphonates. Variance in enzyme complement of a Bacillus in response to growth substrate. Growth of a Bacillus species at the expense of an alkylbenzene sulphonate (ABS) synthetic detergent homologue (1-phenylundecane-p-sulphonate, 11-ABS) containing an odd number of carbon atoms in the alkyl side chain induced an enzyme complement able to biodegrade 11-ABS by alkyl side-chain oxidation, and \"ortho cleavage\" aromatic-ring oxidation reactions. Growth of the Bacillus at the expense of an ABS homologue containing an even number of carbon atoms in the alkyl side chain (1-phenyldodecane-p-sulphonate, 12-ABS) induced an enzyme complement able to biodegrade 12-ABS by alkyl side-chain oxidation, and \"meta cleavage\" aromatic-ring oxidation reactions. The results of a number of different growth and enzyme induction experiments confirm that both 11-ABS and 12-ABS are initially biodegraded by an identical complement of enzymes catalysing the alkyl-side-chain oxidation reactions, but that the subsequent metabolism of the aromatic moieties remaining after the removal of the alkyl side chain from 11-ABS and 12-ABS occurs by two separate pathways requiring the de novo induction of different substrate-specific enzyme complements. The detection of the predicted changes in enzyme complement subsequent to changes in the growth substrate of the Bacillus provide confirmation of the biodegradation pathways operating in the microorganism."}
{"id": "PMID:1086641", "title": "A chemically defined medium for cephalosporin C production by Paecilomyces persicinus.", "content": "A chemically defined medium was developed for the biosynthesis of cephalosporin C by Paecilomyces persicinus Nicot strain P-10. Glucose served as the major carbon source and nitrogen was supplied by five amino acids, L-arginine, L-aspartic acid, L-glutamic acid, glycine and DL-methionine. Omission of any of the first four diminished or prevented production of cephalosporin C; omission of methionine did not. Methionine is not critical for the production of cephalosporin C in this defined medium. Production of the antibiotic was affected by the concentrations of inorganic salts employed. Biotin was required for growth and cephalosporin C synthesis. The addition of L-lysine precursors to the medium did not influence cephalosporin C levels and L-lysine itself inhibited antibiotic production. Known precursors of beta-lactam antibiotics as well as oleic acid did not affect biosynthesis of cephalosporin C. Chemical changes occurring in the defined medium revealed that glucose was efficiently utilized after 96 hours incubation whereas total soluble nitrogen levels increased following an initial sharp decrease. Mycelial weight and cephalosporin C production were both maximal after 96 hours incubation. Mycelial nitrogen was highest after 48 hours incubation whereas mycelial lipid levels were greatest after 72 hours.", "contents": "A chemically defined medium for cephalosporin C production by Paecilomyces persicinus. A chemically defined medium was developed for the biosynthesis of cephalosporin C by Paecilomyces persicinus Nicot strain P-10. Glucose served as the major carbon source and nitrogen was supplied by five amino acids, L-arginine, L-aspartic acid, L-glutamic acid, glycine and DL-methionine. Omission of any of the first four diminished or prevented production of cephalosporin C; omission of methionine did not. Methionine is not critical for the production of cephalosporin C in this defined medium. Production of the antibiotic was affected by the concentrations of inorganic salts employed. Biotin was required for growth and cephalosporin C synthesis. The addition of L-lysine precursors to the medium did not influence cephalosporin C levels and L-lysine itself inhibited antibiotic production. Known precursors of beta-lactam antibiotics as well as oleic acid did not affect biosynthesis of cephalosporin C. Chemical changes occurring in the defined medium revealed that glucose was efficiently utilized after 96 hours incubation whereas total soluble nitrogen levels increased following an initial sharp decrease. Mycelial weight and cephalosporin C production were both maximal after 96 hours incubation. Mycelial nitrogen was highest after 48 hours incubation whereas mycelial lipid levels were greatest after 72 hours."}
{"id": "PMID:1086642", "title": "The ascigerous state of Candida chodatii.", "content": "In Candida chodatii, a mycelial yeast having denticulate conidiogenous cells, the asci are formed by conjugating budding cells (conidia) and usually contain 2 hat-shaped, small ascospores. It is classified in Hyphopichia, a new genus of the Ascoideaceae. A key to the genera of the Ascoideaceae is given.", "contents": "The ascigerous state of Candida chodatii. In Candida chodatii, a mycelial yeast having denticulate conidiogenous cells, the asci are formed by conjugating budding cells (conidia) and usually contain 2 hat-shaped, small ascospores. It is classified in Hyphopichia, a new genus of the Ascoideaceae. A key to the genera of the Ascoideaceae is given."}
{"id": "PMID:1086643", "title": "Cell-wall composition and taxonomy of Cephaloascus fragrans and some Ophiostomataceae.", "content": "Carbohydrates of intact cells and cell walls were studied by gas-liquid chromatographical analysis after acid hydrolysis. Isolated cellulose was determined by infrared spectrophotometry, pyrolysis mass spectrometry and histochemistry. Biochemical characters do not support an assumed relationship between Ophiostoma (including Europhium) and Cephaloascus fragrans. Cephaloascus fragrans differs from Ophiostoma by a high mannose content and by the absence of cellulose and rhamnose. A relationship between Cephaloascus fragrans and Ceratocystis cannot be excluded on the basis of the biochemical characters, although there is a marked difference in conidiogenesis. Saprolegnia ferax (Oomycetes) was included as a cellulose-containing fungus for comparison.", "contents": "Cell-wall composition and taxonomy of Cephaloascus fragrans and some Ophiostomataceae. Carbohydrates of intact cells and cell walls were studied by gas-liquid chromatographical analysis after acid hydrolysis. Isolated cellulose was determined by infrared spectrophotometry, pyrolysis mass spectrometry and histochemistry. Biochemical characters do not support an assumed relationship between Ophiostoma (including Europhium) and Cephaloascus fragrans. Cephaloascus fragrans differs from Ophiostoma by a high mannose content and by the absence of cellulose and rhamnose. A relationship between Cephaloascus fragrans and Ceratocystis cannot be excluded on the basis of the biochemical characters, although there is a marked difference in conidiogenesis. Saprolegnia ferax (Oomycetes) was included as a cellulose-containing fungus for comparison."}
{"id": "PMID:1086646", "title": "Prosthecobacter fusiformis nov. gen. et sp., the fusiform caulobacter.", "content": "Four strains of heterotrophic, fusiform caulobacters have been isolated from freshwater sources. A single prostheca extends from one pole of mature cells, and cells attach to various substrata by means of a holdfast located at the distal tip of the appendage. Thus, superficially these bacteria bear a strong resemblance to bacteria in the genus Caulobacter. However, unlike Caulobacter these bacteria do not exhibit a dimorphic life cycle of motile, non-stalked daughter cells and immotile, stalked mother cells. Instead both mother and daughter cells are immotile, and at the time of cell separation the daughter cells are essentially identical mirror-image replicas of the mother cell. In addition, the prosthecae of these fusiform caulobacters do not have crossbands, they are somewhat wider than the stalks of Caulobacter and the pseudostalks of Asticcacaulis, and they terminate in a bulbous tip. The deoxyribonucleic acid (DNA) base composition ranges from 54.6-60.1, well below the 62-67 range for the genus Caulobacter. Based upon these and other differences a new genus and species, Prosthecobacter fusiformis, is proposed for the fusiform caulobacters.", "contents": "Prosthecobacter fusiformis nov. gen. et sp., the fusiform caulobacter. Four strains of heterotrophic, fusiform caulobacters have been isolated from freshwater sources. A single prostheca extends from one pole of mature cells, and cells attach to various substrata by means of a holdfast located at the distal tip of the appendage. Thus, superficially these bacteria bear a strong resemblance to bacteria in the genus Caulobacter. However, unlike Caulobacter these bacteria do not exhibit a dimorphic life cycle of motile, non-stalked daughter cells and immotile, stalked mother cells. Instead both mother and daughter cells are immotile, and at the time of cell separation the daughter cells are essentially identical mirror-image replicas of the mother cell. In addition, the prosthecae of these fusiform caulobacters do not have crossbands, they are somewhat wider than the stalks of Caulobacter and the pseudostalks of Asticcacaulis, and they terminate in a bulbous tip. The deoxyribonucleic acid (DNA) base composition ranges from 54.6-60.1, well below the 62-67 range for the genus Caulobacter. Based upon these and other differences a new genus and species, Prosthecobacter fusiformis, is proposed for the fusiform caulobacters."}
{"id": "PMID:1086647", "title": "Pichia humboldtii sp. nov., the perfect state of Candida ingens.", "content": "The perfect state of Candida ingens was found after treatment with N-methyl-N'-nitro-N-nitrosoguanidine, a mutagenic agent. The species fits into the genus Pichia. A description of Pichia humboldtii is given.", "contents": "Pichia humboldtii sp. nov., the perfect state of Candida ingens. The perfect state of Candida ingens was found after treatment with N-methyl-N'-nitro-N-nitrosoguanidine, a mutagenic agent. The species fits into the genus Pichia. A description of Pichia humboldtii is given."}
{"id": "PMID:1086648", "title": "The isolation and properties of a denitrifying bacterium of the genus Flavobacterium.", "content": "A previously undescribed denitrifying bacterium was isolated from soil. The cells are small gram-negative rods, asporogenous, and non-motile. Colonies become yellow after long exposure to light. This colouring is due to the production of a carotenoid pigment. The organism shows no fermenting activity, and grows only in the presence of one of the following electron acceptors: NO2, N2O, and O2. It does not reduce nitrate. It gives a positive oxidase test and has a cytochrome c and catalase. It requires no growth factors, is a chemoorganotroph and uses only sugars as carbon and energy supply. The DNA base composition is 40.8 moles percent GC. Although presenting the physiological characteristics of a pseudomonad, the organism described has been placed in the genus Flavobacterium because of its pigmentation and its low GC percentage.", "contents": "The isolation and properties of a denitrifying bacterium of the genus Flavobacterium. A previously undescribed denitrifying bacterium was isolated from soil. The cells are small gram-negative rods, asporogenous, and non-motile. Colonies become yellow after long exposure to light. This colouring is due to the production of a carotenoid pigment. The organism shows no fermenting activity, and grows only in the presence of one of the following electron acceptors: NO2, N2O, and O2. It does not reduce nitrate. It gives a positive oxidase test and has a cytochrome c and catalase. It requires no growth factors, is a chemoorganotroph and uses only sugars as carbon and energy supply. The DNA base composition is 40.8 moles percent GC. Although presenting the physiological characteristics of a pseudomonad, the organism described has been placed in the genus Flavobacterium because of its pigmentation and its low GC percentage."}
{"id": "PMID:1086649", "title": "Heterothallism in Pichia kudriavzevii and Pichia terricola.", "content": "Pichia kudriavzevii and P. terricola were found to be heterothallic, but not interfertile with one another; nor did they mate with P. membranaefaciens, P. scutulata, Candida lambica, C. diversa, C. ingens, C. silvae, C. valida, C. vini, C. norvegensis, or Torulopsis inconspicua. Limited conjugation occurred between mating types of P. kudriavzevii and C. krusei and conjugation and sporulation occurred in mixtures with C. sorbosa. The data indicate C. krusei and C. sorbosa to be the same species and to represent imperfect forms of P. kudriavzevii.", "contents": "Heterothallism in Pichia kudriavzevii and Pichia terricola. Pichia kudriavzevii and P. terricola were found to be heterothallic, but not interfertile with one another; nor did they mate with P. membranaefaciens, P. scutulata, Candida lambica, C. diversa, C. ingens, C. silvae, C. valida, C. vini, C. norvegensis, or Torulopsis inconspicua. Limited conjugation occurred between mating types of P. kudriavzevii and C. krusei and conjugation and sporulation occurred in mixtures with C. sorbosa. The data indicate C. krusei and C. sorbosa to be the same species and to represent imperfect forms of P. kudriavzevii."}
{"id": "PMID:1086651", "title": "Mixed connective tissue disease.", "content": "Three patients with mixed connective tissue disease (MCTD) had clinical features that included a high incidence of Raynaud phenomenon, arthritis, myositis, and swollen hands. The diagnostic laboratory test result was the presence of high titers of antibody to extractable nuclear antigen. These antibody titers are notably reduced or abolished in patients with MCTD when the tanned red blood cells that are used in the test are pretreated with ribonuclease. Speckled antinuclear antibodies were present in all patients. Patients with MCTD have a low incidence of renal disease, are responsive to treatment with prednisone, and have a good prognosis.", "contents": "Mixed connective tissue disease. Three patients with mixed connective tissue disease (MCTD) had clinical features that included a high incidence of Raynaud phenomenon, arthritis, myositis, and swollen hands. The diagnostic laboratory test result was the presence of high titers of antibody to extractable nuclear antigen. These antibody titers are notably reduced or abolished in patients with MCTD when the tanned red blood cells that are used in the test are pretreated with ribonuclease. Speckled antinuclear antibodies were present in all patients. Patients with MCTD have a low incidence of renal disease, are responsive to treatment with prednisone, and have a good prognosis."}
{"id": "PMID:1086653", "title": "Fc-rosette inhibition by hypocomplementaemic systemic lupus erythematosus sera.", "content": "Human red cells sensitized with one of the Rh antisera (Ripley) form rosettes (Fc-rosette) with human B lymphocytes and the rosettes are well inhibited by aggregated human IgG. Since sera of hypocomplementaemic patients with systemic lupus erythematosus (SLE) have frequently been reported to contain immune complexes, they were used for the inhibition of Fc-rosette formation in this study. The results of Fc-rosette inhibition rates of the sera were inversely correlated with the serum CH50 levels. When the sera were separated into top, middle, and bottom fractions by ultracentrifugation, the bottom fractions showed more effective inhititions than the others. Similarly, the strongest inhibition was found in the void volume of the serum separated by Sephadex G200 gel filtration. Reduction and alkylation of IgG resulted in the loss of reactivity with Fc receptor of B lymphocytes, and the rosette inhibiting activities of the SLE sera were markedly reduced after reduction and alkylation. Some of anti-HLA sera were inhibitory for the Fc-rosette formation, while the tested sera did not contain anti-HLA activity assessed by the microcytotoxicity test. These results indicated that circulating immune complexes contained in the sera inhibit the rosette formation, and that the Fc-rosette inhibition test is a simple and relatively sensitive method for the detection of circulating immune complexes. Antinuclear antibody activities of the sera were tested by the indirect immunofluorescent method; however, clear correlations were not obtained between Fc-rosette inhibition rates and staining patterns of antinuclear antibodies. On the other hand, the positive groups of LE-test exhibited slightly greater inhibition rates of the rosette than the negative groups.", "contents": "Fc-rosette inhibition by hypocomplementaemic systemic lupus erythematosus sera. Human red cells sensitized with one of the Rh antisera (Ripley) form rosettes (Fc-rosette) with human B lymphocytes and the rosettes are well inhibited by aggregated human IgG. Since sera of hypocomplementaemic patients with systemic lupus erythematosus (SLE) have frequently been reported to contain immune complexes, they were used for the inhibition of Fc-rosette formation in this study. The results of Fc-rosette inhibition rates of the sera were inversely correlated with the serum CH50 levels. When the sera were separated into top, middle, and bottom fractions by ultracentrifugation, the bottom fractions showed more effective inhititions than the others. Similarly, the strongest inhibition was found in the void volume of the serum separated by Sephadex G200 gel filtration. Reduction and alkylation of IgG resulted in the loss of reactivity with Fc receptor of B lymphocytes, and the rosette inhibiting activities of the SLE sera were markedly reduced after reduction and alkylation. Some of anti-HLA sera were inhibitory for the Fc-rosette formation, while the tested sera did not contain anti-HLA activity assessed by the microcytotoxicity test. These results indicated that circulating immune complexes contained in the sera inhibit the rosette formation, and that the Fc-rosette inhibition test is a simple and relatively sensitive method for the detection of circulating immune complexes. Antinuclear antibody activities of the sera were tested by the indirect immunofluorescent method; however, clear correlations were not obtained between Fc-rosette inhibition rates and staining patterns of antinuclear antibodies. On the other hand, the positive groups of LE-test exhibited slightly greater inhibition rates of the rosette than the negative groups."}
{"id": "PMID:1086654", "title": "Lymphocytes in rheumatoid and nonrheumatoid synovial fluids. Nonspecificity of high T-cell and low B-cell percentages.", "content": "Lymphocytes were studied in paired peripheral blood and synovial fluid samples from patients with various forms of arthritis, including rheumatoid arthritis (group I) and other polyarthritides of unknown origin (group II), as well as arthritides generally considered not to be immunologically mediated, such as crystal synovitis, traumatic arthritis, osteoarthrosis, and pigmented villonodular synovitis (group III). In all 3 groups the percentages of T lymphocytes were significantly higher in synovial fluids than in the peripheral blood, whereas those of the synovial fluid B lymphocytes were consistently very low and occasionally nil. Absolute numbers of synovial fluid lymphocytes were significantly higher in groups I and II as compared with group III, and in the peripheral blood absolute numbers of lymphocytes in groups I and II were significantly lower than in controls. No correlation was found between absolute numbers of lymphocytes and complement activity in the synovial fluid. The characteristic pattern of high T-cell and very low B-cell percentages in synovial fluids is a general feature of inflammatory exudates and cannot be considered an expression of cell-mediated immunity in itself.", "contents": "Lymphocytes in rheumatoid and nonrheumatoid synovial fluids. Nonspecificity of high T-cell and low B-cell percentages. Lymphocytes were studied in paired peripheral blood and synovial fluid samples from patients with various forms of arthritis, including rheumatoid arthritis (group I) and other polyarthritides of unknown origin (group II), as well as arthritides generally considered not to be immunologically mediated, such as crystal synovitis, traumatic arthritis, osteoarthrosis, and pigmented villonodular synovitis (group III). In all 3 groups the percentages of T lymphocytes were significantly higher in synovial fluids than in the peripheral blood, whereas those of the synovial fluid B lymphocytes were consistently very low and occasionally nil. Absolute numbers of synovial fluid lymphocytes were significantly higher in groups I and II as compared with group III, and in the peripheral blood absolute numbers of lymphocytes in groups I and II were significantly lower than in controls. No correlation was found between absolute numbers of lymphocytes and complement activity in the synovial fluid. The characteristic pattern of high T-cell and very low B-cell percentages in synovial fluids is a general feature of inflammatory exudates and cannot be considered an expression of cell-mediated immunity in itself."}
{"id": "PMID:1086655", "title": "Effect of penicillamine therapy on circulating immune complexes in rheumatoid arthritis.", "content": "The sera of 40 patients with severe progressive rheumatoid arthritis were examined for the presence of soluble immune complexes before penicillamine therapy was started, and again after treatment for a mean period of 14-4 months. The methods used were radiobioassay (macrophage uptake), C1q-binding capacity, and precipitation by 4% polyethylene glycol. Before treatment the sera of 37 patients showed significantly enhanced uptake of 125I-labelled aggregated human IgG by guinea pig macrophages. Treatment produced significant falls in mean erythrocyte sedimentation rate, differential agglutination titre, and serum IgG and IgM levels, and enhancing complexes (EC) decreased or disappeared in 20 patients. In 9 patients EC changed to inhibiting complexes, and in 8 EC levels were unchanged. In 6 of 8 patients with cutaneous vasculitis initially, both lesions and EC disappeared. The total protein and the IgG and IgM precipitated from patients' sera by 4% polyethylene glycol fell significantly on treatment. Antinuclear antibody titres were unchanged by penicillamine, and 3 patients acquired these antibodies during treatment. These findings suggest that penicillamine treatment in rheumatoid arthritis reduces the level of circulating soluble immune complexes in which IgM rheumatoid factor is a component.", "contents": "Effect of penicillamine therapy on circulating immune complexes in rheumatoid arthritis. The sera of 40 patients with severe progressive rheumatoid arthritis were examined for the presence of soluble immune complexes before penicillamine therapy was started, and again after treatment for a mean period of 14-4 months. The methods used were radiobioassay (macrophage uptake), C1q-binding capacity, and precipitation by 4% polyethylene glycol. Before treatment the sera of 37 patients showed significantly enhanced uptake of 125I-labelled aggregated human IgG by guinea pig macrophages. Treatment produced significant falls in mean erythrocyte sedimentation rate, differential agglutination titre, and serum IgG and IgM levels, and enhancing complexes (EC) decreased or disappeared in 20 patients. In 9 patients EC changed to inhibiting complexes, and in 8 EC levels were unchanged. In 6 of 8 patients with cutaneous vasculitis initially, both lesions and EC disappeared. The total protein and the IgG and IgM precipitated from patients' sera by 4% polyethylene glycol fell significantly on treatment. Antinuclear antibody titres were unchanged by penicillamine, and 3 patients acquired these antibodies during treatment. These findings suggest that penicillamine treatment in rheumatoid arthritis reduces the level of circulating soluble immune complexes in which IgM rheumatoid factor is a component."}
{"id": "PMID:1086656", "title": "Effect of penicillamine treatment on immune complexes in two cases of seropositive juvenile rheumatoid arthritis.", "content": "A correlation has previously been observed between the presence of enhancing complexes and cutaneous vasculitis in rheumatoid arthritis. Two parients with seropositive juvenile rheumatoid arthritis are described in whom enhancing complexes were detected before the appearance of cutaneous vasculitis. Their contrasting response to penicillamine is discussed in relation to the role of of rheumatoid factor and antinuclear antibodies.", "contents": "Effect of penicillamine treatment on immune complexes in two cases of seropositive juvenile rheumatoid arthritis. A correlation has previously been observed between the presence of enhancing complexes and cutaneous vasculitis in rheumatoid arthritis. Two parients with seropositive juvenile rheumatoid arthritis are described in whom enhancing complexes were detected before the appearance of cutaneous vasculitis. Their contrasting response to penicillamine is discussed in relation to the role of of rheumatoid factor and antinuclear antibodies."}
{"id": "PMID:1086657", "title": "Recognition and operative management of patients with arteriosclerotic coronary artery aneurysms.", "content": "Arteriosclerotic aneurysms of the coronary arteries have been noted in 38 patients undergoing cardiac surgical procedures. Age, sex, and risk factors are similar to those in other patients with coronary artery disease. A high incidence of previous myocardial infarction (24 of 38) is related to the frequency of clot formation and embolism associated with such aneurysms. There is also an increased risk of perioperative infarction following coronary artery bypass grafting if the suture line involves the aneurysm wall (6 of 6), but not when sutures are placed away from it. The importance of recognition and proper operative management is stressed.", "contents": "Recognition and operative management of patients with arteriosclerotic coronary artery aneurysms. Arteriosclerotic aneurysms of the coronary arteries have been noted in 38 patients undergoing cardiac surgical procedures. Age, sex, and risk factors are similar to those in other patients with coronary artery disease. A high incidence of previous myocardial infarction (24 of 38) is related to the frequency of clot formation and embolism associated with such aneurysms. There is also an increased risk of perioperative infarction following coronary artery bypass grafting if the suture line involves the aneurysm wall (6 of 6), but not when sutures are placed away from it. The importance of recognition and proper operative management is stressed."}
{"id": "PMID:1086658", "title": "Combined valvular and coronary artery surgery.", "content": "Between July 1, 1971, and March 1, 1975, 45 patients underwent combined valvular and coronary artery operation. Aortic valve disease was present in 30 patients, mitral valve disease in 13, aortic and mitral valve disease in 1, and tricuspid valve disease in 1. The average age was 57 years. Seventeen patients were in New York Heart Association Functional Class IV. Seventeen patients had had a previous myocardial infarction. Significant coronary artery disease was an unexpected finding at the time of coronary angiography in 14 patients. The average number of grafts inserted was 2.5 per patient. The grafts were placed prior to valve replacement, and periods of myocardial ischemia were kept at a minimum by maintaining coronary perfusion throughout the operation. Operative mortality was 16%; late mortality was 8%. Perioperative myocardial infarction occurred in 2 patients.", "contents": "Combined valvular and coronary artery surgery. Between July 1, 1971, and March 1, 1975, 45 patients underwent combined valvular and coronary artery operation. Aortic valve disease was present in 30 patients, mitral valve disease in 13, aortic and mitral valve disease in 1, and tricuspid valve disease in 1. The average age was 57 years. Seventeen patients were in New York Heart Association Functional Class IV. Seventeen patients had had a previous myocardial infarction. Significant coronary artery disease was an unexpected finding at the time of coronary angiography in 14 patients. The average number of grafts inserted was 2.5 per patient. The grafts were placed prior to valve replacement, and periods of myocardial ischemia were kept at a minimum by maintaining coronary perfusion throughout the operation. Operative mortality was 16%; late mortality was 8%. Perioperative myocardial infarction occurred in 2 patients."}
{"id": "PMID:1086661", "title": "Widespread bone involvement in sinus histiocytosis.", "content": "Sinus histiocytosis with massive lymphadenopathy is a rare disorder of the reticuloendothelial system with predilection for black children.", "contents": "Widespread bone involvement in sinus histiocytosis. Sinus histiocytosis with massive lymphadenopathy is a rare disorder of the reticuloendothelial system with predilection for black children."}
{"id": "PMID:1086662", "title": "Results of reoperation for failed aortocoronary bypass grafts.", "content": "Thirty-one patients underwent reoperation for failure of one or more previous aortocoronary bypass grafts. Thirty-nine grafts were replaced. Twenty-four grafts were constructed to vessels not thought to be significantly diseased at initial operation. There were no early or late deaths. Postoperative morbidity was comparable to initial prodecures. Of 24 patients followed up for more than six months., 62% clearly experienced improvement without evidence of perioperative or postoperative infarction. Reoperation for failed aortocoronary bypass grafts can be achieved without excess risk, but with long-term results less optimistic than initial procedures.", "contents": "Results of reoperation for failed aortocoronary bypass grafts. Thirty-one patients underwent reoperation for failure of one or more previous aortocoronary bypass grafts. Thirty-nine grafts were replaced. Twenty-four grafts were constructed to vessels not thought to be significantly diseased at initial operation. There were no early or late deaths. Postoperative morbidity was comparable to initial prodecures. Of 24 patients followed up for more than six months., 62% clearly experienced improvement without evidence of perioperative or postoperative infarction. Reoperation for failed aortocoronary bypass grafts can be achieved without excess risk, but with long-term results less optimistic than initial procedures."}
{"id": "PMID:1086663", "title": "Acoustic reflex test in neuro-otologic diagnosis. A review of 24 cases of acoustic tumors.", "content": "Acoustic and other tumors of the cerebellopontine angle are not uncommon, and they usually have otologic symptoms or findings. Early diagnosis depends on a high index of suspicion and a thorough evaluation of patients with suspected tumors. This is a report of 24 cases selected from a group of 184 surgically confirmed tumors. Had the cases been evaluated only on the basis of pure tone or speech audiometry or on the basis of plain roentgenograms, the diagnosis might not have been made. Audiological examination should include the acoustic reflex test. The results of this test indicated a retrocochlear problem (absence or decay of the reflex) in 19 of 24 cases (80%). The test was positive in 11 of 12 patients with normal or near normal hearing and in seven of eight patients with normal x-ray films.", "contents": "Acoustic reflex test in neuro-otologic diagnosis. A review of 24 cases of acoustic tumors. Acoustic and other tumors of the cerebellopontine angle are not uncommon, and they usually have otologic symptoms or findings. Early diagnosis depends on a high index of suspicion and a thorough evaluation of patients with suspected tumors. This is a report of 24 cases selected from a group of 184 surgically confirmed tumors. Had the cases been evaluated only on the basis of pure tone or speech audiometry or on the basis of plain roentgenograms, the diagnosis might not have been made. Audiological examination should include the acoustic reflex test. The results of this test indicated a retrocochlear problem (absence or decay of the reflex) in 19 of 24 cases (80%). The test was positive in 11 of 12 patients with normal or near normal hearing and in seven of eight patients with normal x-ray films."}
{"id": "PMID:1086664", "title": "Pathophysiology of endolymphatic hydrops.", "content": "Endolymphatic hydrops of the nonprogressive type occurs in response to a single traumatic or toxic insult of limited duration and although it may result in permanent deficits in sensorineural function, there is total subsidence of vestibular symptoms. Endolymphatic hydrops of the progressive type, on the other hand, appears to be the result of permanent impairment of endolymph resorption and is caused principally by disorders of the endolymphatic sac. It occurs in Meni\u00e8re's disease, syphilitic labyrinthitis and the delayed hydrops syndrome. In addition to deafness of varying extent, it is characterized by episodic vertigo and sometimes by Hennebert's sign. Histological studies suggest that the acute vertiginous episodes are caused by potassium intoxication following ruptures of the membranous labyrinth and that Hennerbert's sign is caused by vestibular fibrosis.", "contents": "Pathophysiology of endolymphatic hydrops. Endolymphatic hydrops of the nonprogressive type occurs in response to a single traumatic or toxic insult of limited duration and although it may result in permanent deficits in sensorineural function, there is total subsidence of vestibular symptoms. Endolymphatic hydrops of the progressive type, on the other hand, appears to be the result of permanent impairment of endolymph resorption and is caused principally by disorders of the endolymphatic sac. It occurs in Meni\u00e8re's disease, syphilitic labyrinthitis and the delayed hydrops syndrome. In addition to deafness of varying extent, it is characterized by episodic vertigo and sometimes by Hennebert's sign. Histological studies suggest that the acute vertiginous episodes are caused by potassium intoxication following ruptures of the membranous labyrinth and that Hennerbert's sign is caused by vestibular fibrosis."}
{"id": "PMID:1086659", "title": "[Determination of T anb B lymphocytes in patients with rheumatic fever].", "content": "The populations of \"T\" and \"B\" lymphocytes were evaluated in the present work employing the rosette technique in patients with active rheumatic fever, diagnosed according to Jones' criterion. \"B\" lymphocytes were found diminished at 7 days after the acute episode of rheumatic fever and increased at 35 days. These findings can be correlated with the hypergammaglobulinemia observed in this type of cases. Although there were wide variations in the quantity of \"T\" lymphocytes in some patients with rheumatic fever in this study, the porcentual and absolute values of \"T\" lymphocytes had no modifications when these were compared with the control group. The patients showed leucocytosis when entered the hospital and 7 days after. At 35 days of hospitalization they presented discrete lymphocytosis. Since the patients with rheumatic fever usually receive anti-inflammatory treatment with steroids, and as these drugs are capable of decreasing selectively the \"T\" lymphocytes, their effect on the kinetics of the lymphocytes in rheumatic fever has to be elavuated in further studies.", "contents": "[Determination of T anb B lymphocytes in patients with rheumatic fever]. The populations of \"T\" and \"B\" lymphocytes were evaluated in the present work employing the rosette technique in patients with active rheumatic fever, diagnosed according to Jones' criterion. \"B\" lymphocytes were found diminished at 7 days after the acute episode of rheumatic fever and increased at 35 days. These findings can be correlated with the hypergammaglobulinemia observed in this type of cases. Although there were wide variations in the quantity of \"T\" lymphocytes in some patients with rheumatic fever in this study, the porcentual and absolute values of \"T\" lymphocytes had no modifications when these were compared with the control group. The patients showed leucocytosis when entered the hospital and 7 days after. At 35 days of hospitalization they presented discrete lymphocytosis. Since the patients with rheumatic fever usually receive anti-inflammatory treatment with steroids, and as these drugs are capable of decreasing selectively the \"T\" lymphocytes, their effect on the kinetics of the lymphocytes in rheumatic fever has to be elavuated in further studies."}
{"id": "PMID:1086665", "title": "Meni\u00e8re's disease - roentgenologic diagnosis.", "content": "Radiologic diagnosis in Meni\u00e8re's disease should not be based on the vestibular aqueducts tomographic reproducibility. Instead, valuable preoperative information should be given about the size and location of the aqueducts external aperture in relation to the posterior and lateral semicircular canals. In saccotomy the semicircular canals are landmarks for identification of the endolymphatic sac. The periaqueductal pneumatization is demonstrated to be partly decisive for the vestibular aqueducts gross morphology. In Meni\u00e8re's disease either a sparse or a total lack of periaqueductal pneumatization is observed in most of all cases. The development of peri- and infralabyrinthine pneumatization is also decisive for the position of the foveate impression, which houses the extraosseous part of the endolymphatic sac. Its location and size can be evaluated by tomographic characterization of the vestibular aqueducts external aperture.", "contents": "Meni\u00e8re's disease - roentgenologic diagnosis. Radiologic diagnosis in Meni\u00e8re's disease should not be based on the vestibular aqueducts tomographic reproducibility. Instead, valuable preoperative information should be given about the size and location of the aqueducts external aperture in relation to the posterior and lateral semicircular canals. In saccotomy the semicircular canals are landmarks for identification of the endolymphatic sac. The periaqueductal pneumatization is demonstrated to be partly decisive for the vestibular aqueducts gross morphology. In Meni\u00e8re's disease either a sparse or a total lack of periaqueductal pneumatization is observed in most of all cases. The development of peri- and infralabyrinthine pneumatization is also decisive for the position of the foveate impression, which houses the extraosseous part of the endolymphatic sac. Its location and size can be evaluated by tomographic characterization of the vestibular aqueducts external aperture."}
{"id": "PMID:1086678", "title": "Thymus-dependent (T) lymphocyte deficiency in progressive systemic sclerosis.", "content": "Circulating thymus-dependent (T) lymphocytes were estimated in twenty-seven patients with progressive systemic sclerosis (PSS) and in forty-five normal controls using the property of T lymphocytes to form rosettes with sheep red blood cells. The patients with PSS were found to have a reduction of T lymphocytes which correlated with the extent of visceral involvement by the disease, those with the lowest counts having the most extensive disease. These findings support the suggestion that immunological factors may be involved in the pathogenesis of PSS.", "contents": "Thymus-dependent (T) lymphocyte deficiency in progressive systemic sclerosis. Circulating thymus-dependent (T) lymphocytes were estimated in twenty-seven patients with progressive systemic sclerosis (PSS) and in forty-five normal controls using the property of T lymphocytes to form rosettes with sheep red blood cells. The patients with PSS were found to have a reduction of T lymphocytes which correlated with the extent of visceral involvement by the disease, those with the lowest counts having the most extensive disease. These findings support the suggestion that immunological factors may be involved in the pathogenesis of PSS."}
{"id": "PMID:1086679", "title": "Generalized warts and immune deficiency.", "content": "A case of common variable hypogammaglobulinaemia with associated impairment of cell mediated immunity and severe wart virus infection is described. The defect of cell mediated immunity is thought to have predisposed this patient to the development of persistent wart infection which in turn grossly depressed the body's cellular immunity and thus allowed widespread dissemination of the warts. The rapid restoration of cell mediated immunity which followed the reduction in the antigenic load of wart virus by diathermy treatment was followed by the spontaneous regression of all the patient's warts. This unusual case may provide some insight into the complex relationship between wart virus infection and the immune system of the host.", "contents": "Generalized warts and immune deficiency. A case of common variable hypogammaglobulinaemia with associated impairment of cell mediated immunity and severe wart virus infection is described. The defect of cell mediated immunity is thought to have predisposed this patient to the development of persistent wart infection which in turn grossly depressed the body's cellular immunity and thus allowed widespread dissemination of the warts. The rapid restoration of cell mediated immunity which followed the reduction in the antigenic load of wart virus by diathermy treatment was followed by the spontaneous regression of all the patient's warts. This unusual case may provide some insight into the complex relationship between wart virus infection and the immune system of the host."}
{"id": "PMID:1086681", "title": "Unsuspected fungal endophthalmitis diagnosed in vitrectomy specimen.", "content": "A vitrectomy specimen obtained with the roto-extractor from a diabetic patient with vitreous haemorrhage revealed fungal endophthalmitis on microscopical examination. Endophthalmitis was not suspected before the procedure. Extensive medical examinations failed to reveal any evidence of systemic fungal disease. The case exemplifies endogenous mycotic endophthalmitis of occult origin, the first of its kind, we believe, to be diagnosed in this manner.", "contents": "Unsuspected fungal endophthalmitis diagnosed in vitrectomy specimen. A vitrectomy specimen obtained with the roto-extractor from a diabetic patient with vitreous haemorrhage revealed fungal endophthalmitis on microscopical examination. Endophthalmitis was not suspected before the procedure. Extensive medical examinations failed to reveal any evidence of systemic fungal disease. The case exemplifies endogenous mycotic endophthalmitis of occult origin, the first of its kind, we believe, to be diagnosed in this manner."}
{"id": "PMID:1086682", "title": "Decreased rate of sodium conductance inactivation in the node of Ranvier induced by a polypeptide toxin from sea anemone.", "content": "The effects of two toxins extracted from the tentacles of Anemonia sulcata on ionic currents have been tested on the nodal membrane of myelinated nerve fibres from Rana esculenta. While external application of Toxin I at 100 muM leaves both specific ionic currents unmodified, Toxin II at 10 muM reacts with a receptor site associated with the sodium conductance inactivation gating. Since internal application by diffusion of Toxin II at a concentration of 700 muM leaves the ionic currents unchanged, the receptor site is most likely located on the external side of the nodal membrane. An equilibrium dissociation constant for the effects of Toxin II was estimated as 20 muM. The on-reaction is fast (rate constant for the on-reaction roughly equal to 3.103 M-1) suggesting a readily accesible receptor site for the toxin. The kinetics characteristics of the sodium currents recorded in the presence of Toxin II suggest that there are at least two steps in the reaction leading to Na+ -channels with the inactivation gate completely immobilized. The relatively fast reversibility of the intermediate stage of the reaction and the rather slow but, in the end, complete reversal of the toxin effects suggest that the toxin acts by modifying the energy profile for the transition \"inactivation gate in the open configuration to inactivation gate in the closed configuration.\" Toxin II at higher concentrations (greater than 100 muM) also inhibits the potassium currents but these effects were not studied in any detail.", "contents": "Decreased rate of sodium conductance inactivation in the node of Ranvier induced by a polypeptide toxin from sea anemone. The effects of two toxins extracted from the tentacles of Anemonia sulcata on ionic currents have been tested on the nodal membrane of myelinated nerve fibres from Rana esculenta. While external application of Toxin I at 100 muM leaves both specific ionic currents unmodified, Toxin II at 10 muM reacts with a receptor site associated with the sodium conductance inactivation gating. Since internal application by diffusion of Toxin II at a concentration of 700 muM leaves the ionic currents unchanged, the receptor site is most likely located on the external side of the nodal membrane. An equilibrium dissociation constant for the effects of Toxin II was estimated as 20 muM. The on-reaction is fast (rate constant for the on-reaction roughly equal to 3.103 M-1) suggesting a readily accesible receptor site for the toxin. The kinetics characteristics of the sodium currents recorded in the presence of Toxin II suggest that there are at least two steps in the reaction leading to Na+ -channels with the inactivation gate completely immobilized. The relatively fast reversibility of the intermediate stage of the reaction and the rather slow but, in the end, complete reversal of the toxin effects suggest that the toxin acts by modifying the energy profile for the transition \"inactivation gate in the open configuration to inactivation gate in the closed configuration.\" Toxin II at higher concentrations (greater than 100 muM) also inhibits the potassium currents but these effects were not studied in any detail."}
{"id": "PMID:1086685", "title": "Steroid contraceptive agents and immunological reactivity in experimental animals.", "content": "The immunodepressive activity of widely employed steroid contraceptive agents (SCA's) was evaluated in mice and rats on various humoral and cell-mediated responses. Three different progestagens (lynestrenol, norethindrone or norethynodrel) were administered in combination with the estrogen mestrahese SCA's could significantly reduce the severity of allergic encephalomyelitis in rats, variations in their capacity to interfere with primary response to sheep erythrocytes in mice and anti-mouse erythrocyte autoantibody formation were found. Treatments employed with these SCA's did not reduce tumor allograft resistance in mice and the anti-sheep erythrocyte response in rats. In addition, otherwise immunosuppressive treatments with these SCA's did not modify the mouse response to a T-independent antigen. The possible clinical significance of these results is discussed.", "contents": "Steroid contraceptive agents and immunological reactivity in experimental animals. The immunodepressive activity of widely employed steroid contraceptive agents (SCA's) was evaluated in mice and rats on various humoral and cell-mediated responses. Three different progestagens (lynestrenol, norethindrone or norethynodrel) were administered in combination with the estrogen mestrahese SCA's could significantly reduce the severity of allergic encephalomyelitis in rats, variations in their capacity to interfere with primary response to sheep erythrocytes in mice and anti-mouse erythrocyte autoantibody formation were found. Treatments employed with these SCA's did not reduce tumor allograft resistance in mice and the anti-sheep erythrocyte response in rats. In addition, otherwise immunosuppressive treatments with these SCA's did not modify the mouse response to a T-independent antigen. The possible clinical significance of these results is discussed."}
{"id": "PMID:1086686", "title": "Pulsed nuclear magnetic resonance study of 39K in frog striated muscle.", "content": "Samples of 1 M KCl solution and 10 samples of intact frog striated muscle were studied at 4-7 degrees C and/or at 21-22 degrees C. Field inhomogeneity was minimized by using small sample volumes and by using a superconducting magnet designed specifically to provide highly homogeneous fields. In the present experiments, magnetic field inhomogeneity was measured to contribute less than 15% to the free induction decay observed for intracellular 39K. The signal-to-noise ratio of the measurements was enhanced by means of extensive time-averaging. The rates of nuclear relaxation for 39K in aqueous solution were 22 +/- 3 (mean +/- 95% confidence limits) s-1 at 4-7 degrees C and 15 +/- 2 s-1 at 21-22 degrees C. For intracellular 39K, (1/T2) was measured to be 327 +/- 22 s-1 and 229 +/- 10 s-1 at the lower and higher temperatures, respectively. The corresponding values for (1/T1) in the same muscle samples were 198 +/- 31 s-1 and 79 +/- 15 s-1 at 4-7 degrees C and at 21-22 degrees C, respectively. These results for 39K are similar to those previously obtained for intracellular 23Na. Since less than 1% of the intracellular 23Na has been estimated to be immobilized, fractional immobilization of intracellular 39K is also likely to be insubstantial.", "contents": "Pulsed nuclear magnetic resonance study of 39K in frog striated muscle. Samples of 1 M KCl solution and 10 samples of intact frog striated muscle were studied at 4-7 degrees C and/or at 21-22 degrees C. Field inhomogeneity was minimized by using small sample volumes and by using a superconducting magnet designed specifically to provide highly homogeneous fields. In the present experiments, magnetic field inhomogeneity was measured to contribute less than 15% to the free induction decay observed for intracellular 39K. The signal-to-noise ratio of the measurements was enhanced by means of extensive time-averaging. The rates of nuclear relaxation for 39K in aqueous solution were 22 +/- 3 (mean +/- 95% confidence limits) s-1 at 4-7 degrees C and 15 +/- 2 s-1 at 21-22 degrees C. For intracellular 39K, (1/T2) was measured to be 327 +/- 22 s-1 and 229 +/- 10 s-1 at the lower and higher temperatures, respectively. The corresponding values for (1/T1) in the same muscle samples were 198 +/- 31 s-1 and 79 +/- 15 s-1 at 4-7 degrees C and at 21-22 degrees C, respectively. These results for 39K are similar to those previously obtained for intracellular 23Na. Since less than 1% of the intracellular 23Na has been estimated to be immobilized, fractional immobilization of intracellular 39K is also likely to be insubstantial."}
{"id": "PMID:1086687", "title": "[Specificity of the effect of growth hormone on DNA concentration in the nuclei of the lymphoid cells of hypophysectomized rats].", "content": "The effect of growth hormone on the DNA content in the nuclei of the thymus, spleen and the lymph node lymphocytes was studied by means of cytophotometry. In hypophysectomized rats the growth hormone increased the DNA content in the nuclei of the middle lymphocytes of these organs without altering its amount in the small lymphocytes. Thymus lymphocytes were the most sensitive to the hormone action. The DNA content in the nuclei of these cells increased as soon as one hour after the administration of the hormone; in 4 hours it reached the maximum. Other hormones with an anabolic effect (insulin, thyroxin, testosterone), induced no elevation of DNA in the thymocyte nuclei at that period of time. A conclusion was drawn on the high tropicity of the growth hormone to the cells of the lymphoid organs and particularly to the thymocytes (middle lymphocytes of the thymus).", "contents": "[Specificity of the effect of growth hormone on DNA concentration in the nuclei of the lymphoid cells of hypophysectomized rats]. The effect of growth hormone on the DNA content in the nuclei of the thymus, spleen and the lymph node lymphocytes was studied by means of cytophotometry. In hypophysectomized rats the growth hormone increased the DNA content in the nuclei of the middle lymphocytes of these organs without altering its amount in the small lymphocytes. Thymus lymphocytes were the most sensitive to the hormone action. The DNA content in the nuclei of these cells increased as soon as one hour after the administration of the hormone; in 4 hours it reached the maximum. Other hormones with an anabolic effect (insulin, thyroxin, testosterone), induced no elevation of DNA in the thymocyte nuclei at that period of time. A conclusion was drawn on the high tropicity of the growth hormone to the cells of the lymphoid organs and particularly to the thymocytes (middle lymphocytes of the thymus)."}
{"id": "PMID:1086688", "title": "E1Lymphocytopenia in pseudo-lupus-erythmetaosus syndrome.", "content": "Seven female patients with pseudo-lupus erythematosus (LE)-syndrome had markedly reduced lymphocyte counts in their peripheral blood during the active phase of the disease. One patient, we were able to study during the active phase of her disease, had a diminuation of spontaneous rosettes-forming (T-)lymphocytes, using neuraminidase-treated sheep red blood cells for the test. In this case the percentage of surface-immunoglobulin-bearing (B-)lymphocytes determined by an indirect immunofluorescence technique was augmented. In comparison with 20 normal controls 6 other patients did not show any alterations in the relation of B- and T-lymphocytes in the peripheral blood. 5 patients had circulating lymphocytotoxic antibodies in their serum. A specificity of these antibodies for T-lymphocytes could not be realized.", "contents": "E1Lymphocytopenia in pseudo-lupus-erythmetaosus syndrome. Seven female patients with pseudo-lupus erythematosus (LE)-syndrome had markedly reduced lymphocyte counts in their peripheral blood during the active phase of the disease. One patient, we were able to study during the active phase of her disease, had a diminuation of spontaneous rosettes-forming (T-)lymphocytes, using neuraminidase-treated sheep red blood cells for the test. In this case the percentage of surface-immunoglobulin-bearing (B-)lymphocytes determined by an indirect immunofluorescence technique was augmented. In comparison with 20 normal controls 6 other patients did not show any alterations in the relation of B- and T-lymphocytes in the peripheral blood. 5 patients had circulating lymphocytotoxic antibodies in their serum. A specificity of these antibodies for T-lymphocytes could not be realized."}
{"id": "PMID:1086689", "title": "Lysosomal acid phosphatase: difference between normal and chronic lymphocytic leukaemia T and B lymphocytes.", "content": "Lysosomal acid phosphatase was assayed in homogenates of isolated normal and B cell type chronic lymphocytic leukaemia (B-CLL) T and B lymphocytes by biochemical means. Unlike the results of cytochemical studies reported in the literature enzyme activity was considerably higher in normal B lymphocytes than in corresponding T cells. This finding offers the possibility to use acid phosphatase as a marker for normal B lymphocytes. The diminution of acid phosphatase in unseparated B-CLL lymphocytes depends predominantly upon a loss of enzyme activity in the B cell fraction indicating an intrinsic abnormality of these neoplastic lymphocytes.", "contents": "Lysosomal acid phosphatase: difference between normal and chronic lymphocytic leukaemia T and B lymphocytes. Lysosomal acid phosphatase was assayed in homogenates of isolated normal and B cell type chronic lymphocytic leukaemia (B-CLL) T and B lymphocytes by biochemical means. Unlike the results of cytochemical studies reported in the literature enzyme activity was considerably higher in normal B lymphocytes than in corresponding T cells. This finding offers the possibility to use acid phosphatase as a marker for normal B lymphocytes. The diminution of acid phosphatase in unseparated B-CLL lymphocytes depends predominantly upon a loss of enzyme activity in the B cell fraction indicating an intrinsic abnormality of these neoplastic lymphocytes."}
{"id": "PMID:1086691", "title": "Toxicity of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in larval and adult forms of Rana catesbeiana.", "content": "Varying doses of TCDD ranging from 25 to 1000 mug/kg were administered to the larval and adult forms of the American bullfrog, Rana catesbeiana. Doses of TCDD as high as 1 mg/kg failed to have any significant effect upon survival or completion of metamorphosis in tadpole and doses of up to 500 mug/kg had no effect on survival of adult frogs. Histopathological examination of various tissues from the metamorphosed tadpoles and adult frogs failed to show any abnormalities.", "contents": "Toxicity of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in larval and adult forms of Rana catesbeiana. Varying doses of TCDD ranging from 25 to 1000 mug/kg were administered to the larval and adult forms of the American bullfrog, Rana catesbeiana. Doses of TCDD as high as 1 mg/kg failed to have any significant effect upon survival or completion of metamorphosis in tadpole and doses of up to 500 mug/kg had no effect on survival of adult frogs. Histopathological examination of various tissues from the metamorphosed tadpoles and adult frogs failed to show any abnormalities."}
{"id": "PMID:1086690", "title": "[Recent data on the immunology of tumors. Mechanisms of escape of immunological control. Role of suppressor cells].", "content": "Cells in spleens from tumor bearing animals were found to inhibit in vitro reactivity of lymphocytes to a variety of stimulants. These suppressor cells inhibited the stimulation of normal lymphocytes by mitogens : P.H.A.-Con A-L.P.S. They were adherent cells, bore immunoglobulins at their surface and did not bear the 0 marker. They were also demonstrated in T deprived tumor bearing animals. They might be B cells, or, more probably, macrophages. Other activities that are inhibited by the suppressor cells are: -reactivity to allogenic cells in the mixed lymphocyte culture, -in vitro generation of secondary anti tumor cytotoxic effector cells, -macrophage migration inhibition in the presence of immune lymphocytes and tumor extracts, -reactivity against syngeneic tumor cells or tumor extracts. Suppressor cells may play a role in the general immunodepression of tumor bearing hosts, and in the inability of tumor bearing hosts to reject the tumor. However, the biological role of these non T suppressor cells is not clear, as they also inhibit tumor cell growth, and in vivo experiments favour suppressor cells of thymic origin.", "contents": "[Recent data on the immunology of tumors. Mechanisms of escape of immunological control. Role of suppressor cells]. Cells in spleens from tumor bearing animals were found to inhibit in vitro reactivity of lymphocytes to a variety of stimulants. These suppressor cells inhibited the stimulation of normal lymphocytes by mitogens : P.H.A.-Con A-L.P.S. They were adherent cells, bore immunoglobulins at their surface and did not bear the 0 marker. They were also demonstrated in T deprived tumor bearing animals. They might be B cells, or, more probably, macrophages. Other activities that are inhibited by the suppressor cells are: -reactivity to allogenic cells in the mixed lymphocyte culture, -in vitro generation of secondary anti tumor cytotoxic effector cells, -macrophage migration inhibition in the presence of immune lymphocytes and tumor extracts, -reactivity against syngeneic tumor cells or tumor extracts. Suppressor cells may play a role in the general immunodepression of tumor bearing hosts, and in the inability of tumor bearing hosts to reject the tumor. However, the biological role of these non T suppressor cells is not clear, as they also inhibit tumor cell growth, and in vivo experiments favour suppressor cells of thymic origin."}
{"id": "PMID:1086695", "title": "Effects of haemorrhage upon surgical operations.", "content": "The complications of emergency surgery for acute upper gastro-intestinal haemorrhage in 95 patients were compared with those of surgery for non-bleeding benign peptic ulceration in 380 patients. There was a significantly higher number of senior surgeons performing emergency surgery for haemorrhage. There was a significantly higher mortality (10-5 %), wound dehiscence rate (12-6 %) and wound complication rate with re-bleeding (33-7 %) in the post-haemorrhage patients compared with the non-bleeding group (1-3, 1-8 and 11-6 % respectively). There was no significant difference in the incidence of chest infection in the two groups. Mortality and morbidity after haemorrhage were correlated directly with the amount of blood transfused before surgery. Rats were used to study the effect of the removal of 13 % of the blood volume, and its restoration after varying time intervals, upon subsequent wound healing. Abdominal wounds were tested at 7 days for breaking and bursting strength. There was no difference between the wound strength of the control animals and animals which had been bled for 4 minutes before wounding. A significant reduction of wound strength occurred in animals which had been bled for 30-60 minutes before wounding. Prolonged blood loss was associated with deficient wound healing even if blood loss was restored before the wounds were made. Stored blood may be deficient in a factor which is necessary for normal wound healing.", "contents": "Effects of haemorrhage upon surgical operations. The complications of emergency surgery for acute upper gastro-intestinal haemorrhage in 95 patients were compared with those of surgery for non-bleeding benign peptic ulceration in 380 patients. There was a significantly higher number of senior surgeons performing emergency surgery for haemorrhage. There was a significantly higher mortality (10-5 %), wound dehiscence rate (12-6 %) and wound complication rate with re-bleeding (33-7 %) in the post-haemorrhage patients compared with the non-bleeding group (1-3, 1-8 and 11-6 % respectively). There was no significant difference in the incidence of chest infection in the two groups. Mortality and morbidity after haemorrhage were correlated directly with the amount of blood transfused before surgery. Rats were used to study the effect of the removal of 13 % of the blood volume, and its restoration after varying time intervals, upon subsequent wound healing. Abdominal wounds were tested at 7 days for breaking and bursting strength. There was no difference between the wound strength of the control animals and animals which had been bled for 4 minutes before wounding. A significant reduction of wound strength occurred in animals which had been bled for 30-60 minutes before wounding. Prolonged blood loss was associated with deficient wound healing even if blood loss was restored before the wounds were made. Stored blood may be deficient in a factor which is necessary for normal wound healing."}
{"id": "PMID:1086696", "title": "Multidisciplinary treatment for macrometastatic osteogenic sarcoma.", "content": "Using a co-ordinated multidisciplinary approach with surgery, radiation therapy, and chemotherapy, 14 out of 21 patients with metastases from osteogenic sarcoma were rendered free of disease for over two to over 18 months. Most patients had pulmonary metastases, two had bony metastases, and one had metastases in the iliac nodes. As part of this multidisciplinary approach weekly high-dose methotrexate was given and caused tumour regression in seven out of 15 patients. After all clinical evidence of disease had been removed high-dose methotrexate was administered every two to three weeks as maintenance treatment. To assess the efficacy of treatment the results were compared with those in a historical control group of 82 consecutive patients who developed pulmonary or other metastases. The results in the study group were significantly better. This experience may be similar to that in Wilms's tumour, where actinomycin D has increased the cure rate when administered as adjuvant therapy after treatment of localised or overt metastatic disease.", "contents": "Multidisciplinary treatment for macrometastatic osteogenic sarcoma. Using a co-ordinated multidisciplinary approach with surgery, radiation therapy, and chemotherapy, 14 out of 21 patients with metastases from osteogenic sarcoma were rendered free of disease for over two to over 18 months. Most patients had pulmonary metastases, two had bony metastases, and one had metastases in the iliac nodes. As part of this multidisciplinary approach weekly high-dose methotrexate was given and caused tumour regression in seven out of 15 patients. After all clinical evidence of disease had been removed high-dose methotrexate was administered every two to three weeks as maintenance treatment. To assess the efficacy of treatment the results were compared with those in a historical control group of 82 consecutive patients who developed pulmonary or other metastases. The results in the study group were significantly better. This experience may be similar to that in Wilms's tumour, where actinomycin D has increased the cure rate when administered as adjuvant therapy after treatment of localised or overt metastatic disease."}
{"id": "PMID:1086699", "title": "Surface morphology of the bullfrog amphibian papilla.", "content": "The bullfrog amphibian papilla, which has been identified as the peripheral source of two of the three types of auditory axon in that animal, is shown to have a continual distribution of receptor types based on surface topography, but a dichotomy of types based on the adjacent tectorium and its apparent mechanical linkage to the receptors and their supporting cells and a second dichotomy based on receptor polarization patterns. Each dichotomy divides the receptors into two approximately equal populations and thus might correspond to the previously observed division of primary axons into two frequency-response types.", "contents": "Surface morphology of the bullfrog amphibian papilla. The bullfrog amphibian papilla, which has been identified as the peripheral source of two of the three types of auditory axon in that animal, is shown to have a continual distribution of receptor types based on surface topography, but a dichotomy of types based on the adjacent tectorium and its apparent mechanical linkage to the receptors and their supporting cells and a second dichotomy based on receptor polarization patterns. Each dichotomy divides the receptors into two approximately equal populations and thus might correspond to the previously observed division of primary axons into two frequency-response types."}
{"id": "PMID:1086701", "title": "Tonic inhibitory influence of a supraspinal monoaminergic system on recurrent inhibition of an extensor monosynaptic reflex.", "content": "Recurrent inhibition of the extensor (quadriceps) monosynaptic reflex (MSR) was antagonized by a 5-hydroxytryptamine (5-HT) precursor, 5-hydroxytryptophan (5-HTP, 75 mg/kg), and a specific 5-HT neuronal uptake blocker, fluoxetine-HC1 (Lilly 110140, 0.25-6 mg/kg), in unanaesthetized decerebrate cats. This inhibition of the flexor (posterior biceps-semitendinosus) MSR was not altered by fluoxetine. Cyproheptadine-HC1 (5 mg/kg) partially reversed the above blocking actions of 5-HTP and fluoxetine and a thoracic \"cold block\", which eliminates supraspinal inputs to the caudal spinal cord, also eliminated the blockade by fluoxetine on recurrent inhibition. Cyproheptadine (2.5-5 mg/kg) or phenoxybenzamine-HC1 (2.5-5 mg/kg), administered alone, enhanced recurrent inhibition of the extensor but not of the flexor MSR. Since a \"cold block\" increased recurrent inhibition of the extensor reflex in control animals but failed to alter the inhibition in animals pretreated with either DL-p-chlorophenylalanine (300 mg/kg i.p. for 2 consecutive days) or DL-a-methyl-p-tyrosine methyl ester-HC1 (125 mg/kg i.p. 16 and 4 h prior to experiment), the monoaminergic system would appear to be tonically active. In addition the neuronal uptake blocker, imipramine-HC1 (0.125-4 mg/kg), was more potent in antagonizing recurrent inhibition when injected intra-arterially to the spinal cord than when administered intra-arterially to the brain stem or intravenously, indicating that this agent acts in the spinal cord to block the inhibition. These results support our previous proposal (ref. 18) that a supraspinal system involving 5-HT and noradrenaline antagonizes recurrent inhibition of the quadriceps MSR. This monoaminergic system is tonically active with the 5-HT nerve terminals located in the spinal cord.", "contents": "Tonic inhibitory influence of a supraspinal monoaminergic system on recurrent inhibition of an extensor monosynaptic reflex. Recurrent inhibition of the extensor (quadriceps) monosynaptic reflex (MSR) was antagonized by a 5-hydroxytryptamine (5-HT) precursor, 5-hydroxytryptophan (5-HTP, 75 mg/kg), and a specific 5-HT neuronal uptake blocker, fluoxetine-HC1 (Lilly 110140, 0.25-6 mg/kg), in unanaesthetized decerebrate cats. This inhibition of the flexor (posterior biceps-semitendinosus) MSR was not altered by fluoxetine. Cyproheptadine-HC1 (5 mg/kg) partially reversed the above blocking actions of 5-HTP and fluoxetine and a thoracic \"cold block\", which eliminates supraspinal inputs to the caudal spinal cord, also eliminated the blockade by fluoxetine on recurrent inhibition. Cyproheptadine (2.5-5 mg/kg) or phenoxybenzamine-HC1 (2.5-5 mg/kg), administered alone, enhanced recurrent inhibition of the extensor but not of the flexor MSR. Since a \"cold block\" increased recurrent inhibition of the extensor reflex in control animals but failed to alter the inhibition in animals pretreated with either DL-p-chlorophenylalanine (300 mg/kg i.p. for 2 consecutive days) or DL-a-methyl-p-tyrosine methyl ester-HC1 (125 mg/kg i.p. 16 and 4 h prior to experiment), the monoaminergic system would appear to be tonically active. In addition the neuronal uptake blocker, imipramine-HC1 (0.125-4 mg/kg), was more potent in antagonizing recurrent inhibition when injected intra-arterially to the spinal cord than when administered intra-arterially to the brain stem or intravenously, indicating that this agent acts in the spinal cord to block the inhibition. These results support our previous proposal (ref. 18) that a supraspinal system involving 5-HT and noradrenaline antagonizes recurrent inhibition of the quadriceps MSR. This monoaminergic system is tonically active with the 5-HT nerve terminals located in the spinal cord."}
{"id": "PMID:1086703", "title": "Evidence for an ascending inhibitory histaminergic pathway to the cerebral cortex.", "content": "Previous observations from our laboratory indicate that metiamide is a specific histamine antagonist in rat cerebral cortex. In view of the recent finding that histamine levels and L-histidine decarboxylase (EC 4.1.1.22) activity in cerebral cortex decrease following disruption of the ipsilateral medial forebrain bundle (MFB), the present investigation was undertaken to examine whether iontophoretically applied metiamide antagonizes the inhibition of deep cerebral cortical neurones produced by stimulation of the MFB. In rats anaesthetized with a mixture of methoxyflurane, nitrous oxide and oxygen, stimulation of the ipsilateral MFB or the cortical surface with iontophoretically applied histamine depressed the firing of cortical neurones. Metiamide antagonized the histamine-induced depression and reduced the duration of inhibition produced by MFB stimulation. However, it did not alter the inhibition induced by the cortical surface stimulation. These results indicate that a histaminergic pathway ascending through the MFB may inhibit rat cerebral cortical neurones.", "contents": "Evidence for an ascending inhibitory histaminergic pathway to the cerebral cortex. Previous observations from our laboratory indicate that metiamide is a specific histamine antagonist in rat cerebral cortex. In view of the recent finding that histamine levels and L-histidine decarboxylase (EC 4.1.1.22) activity in cerebral cortex decrease following disruption of the ipsilateral medial forebrain bundle (MFB), the present investigation was undertaken to examine whether iontophoretically applied metiamide antagonizes the inhibition of deep cerebral cortical neurones produced by stimulation of the MFB. In rats anaesthetized with a mixture of methoxyflurane, nitrous oxide and oxygen, stimulation of the ipsilateral MFB or the cortical surface with iontophoretically applied histamine depressed the firing of cortical neurones. Metiamide antagonized the histamine-induced depression and reduced the duration of inhibition produced by MFB stimulation. However, it did not alter the inhibition induced by the cortical surface stimulation. These results indicate that a histaminergic pathway ascending through the MFB may inhibit rat cerebral cortical neurones."}
{"id": "PMID:1086704", "title": "Royal College Lecture, 1976. Preoperative left ventricular ejection fraction and survival after coronary artery surgery.", "content": "A preoperative ejection fraction (EF) of less than 0.30 slightly increases the immediate risk of myocardial revascularization. This risk can be greatly reduced by better myocardial protection and complete revascularization of the coronary lesions during surgery. Poor ventricular function, however, greatly influences the patient's long-term survival, especially when the EF is less than 0.30. In patients with coronary artery disease, in whom there are clinical and angiographic indications of an aortocoronary shunt and the EF, when measured in the right anterior oblique plane, is equal to or greater than 0.30, surgery may be performed with an acceptable surgical risk (3.9%) and satisfactory long-term (4- to 5-yr) survival (85%). When the EF is less than 0.30 it is necessary (before refusing operation) to conduct additional studies of venticular function such as biplane ventriculography, venticulography following the administration of nitroglycerin, epinephrine or after an artificially induced extrasystole. Isotope studies may also be considered, as they can reveal the presence of viable myocardial cells in the hypo- or akinetic segments.", "contents": "Royal College Lecture, 1976. Preoperative left ventricular ejection fraction and survival after coronary artery surgery. A preoperative ejection fraction (EF) of less than 0.30 slightly increases the immediate risk of myocardial revascularization. This risk can be greatly reduced by better myocardial protection and complete revascularization of the coronary lesions during surgery. Poor ventricular function, however, greatly influences the patient's long-term survival, especially when the EF is less than 0.30. In patients with coronary artery disease, in whom there are clinical and angiographic indications of an aortocoronary shunt and the EF, when measured in the right anterior oblique plane, is equal to or greater than 0.30, surgery may be performed with an acceptable surgical risk (3.9%) and satisfactory long-term (4- to 5-yr) survival (85%). When the EF is less than 0.30 it is necessary (before refusing operation) to conduct additional studies of venticular function such as biplane ventriculography, venticulography following the administration of nitroglycerin, epinephrine or after an artificially induced extrasystole. Isotope studies may also be considered, as they can reveal the presence of viable myocardial cells in the hypo- or akinetic segments."}
{"id": "PMID:1086705", "title": "A case of T-cell lymphoma with convoluted lymphocytes.", "content": "A case of T-cell lymphoma, as defined by immunologic studies of lymph node, peripheral blood, and cultured cells, is presented. Convoluted lymphocytes were noted in the original lymph node biopsy, in cerebral spinal fluid preparations and terminally, in the peripheral blood. The prominent neuologic abnormalities representing both central and peripheral nervous system involvement were atypical. Other features included skin and testicular infiltration, leukemic transformation, and refractoriness to therapy. A somewhat similar clinical picture has been reported in other patients with diffuse, poorly differentiated lymphocytic lymphomas, some of which have proven to have a T-cell origin. T-cell lymphoma may represent a distinct clinical entity that calls for modifications of our traditional therapeutic approaches.", "contents": "A case of T-cell lymphoma with convoluted lymphocytes. A case of T-cell lymphoma, as defined by immunologic studies of lymph node, peripheral blood, and cultured cells, is presented. Convoluted lymphocytes were noted in the original lymph node biopsy, in cerebral spinal fluid preparations and terminally, in the peripheral blood. The prominent neuologic abnormalities representing both central and peripheral nervous system involvement were atypical. Other features included skin and testicular infiltration, leukemic transformation, and refractoriness to therapy. A somewhat similar clinical picture has been reported in other patients with diffuse, poorly differentiated lymphocytic lymphomas, some of which have proven to have a T-cell origin. T-cell lymphoma may represent a distinct clinical entity that calls for modifications of our traditional therapeutic approaches."}
{"id": "PMID:1086706", "title": "Sinus histiocytosis with massive lymphadenopathy and spinal epidural involvement: a case report and review of the literature.", "content": "A case of \"sinus histiocytosis with massive lymphoadenopathy\" (SHML) in which spinal epidural involvement caused paraplegia is described. The literature pertaining to this newly recognized entity is reviewed; this review indicates that the process is not necessarily restricted to the lymph nodes and may be progressive in nature. The case we report is the first with histologically proved bone involvement.", "contents": "Sinus histiocytosis with massive lymphadenopathy and spinal epidural involvement: a case report and review of the literature. A case of \"sinus histiocytosis with massive lymphoadenopathy\" (SHML) in which spinal epidural involvement caused paraplegia is described. The literature pertaining to this newly recognized entity is reviewed; this review indicates that the process is not necessarily restricted to the lymph nodes and may be progressive in nature. The case we report is the first with histologically proved bone involvement."}
{"id": "PMID:1086707", "title": "Serum alpha1-antitrypsin in patients with lung cancer or abnormal sputum cytology.", "content": "Serum alpha1-antitrypsin Pi types and trypsin inhibitory capacity (TIC) were measured in 72 patients with lung cancer and in 196 patients with abnormal sputum cytology but no clinical evidence of lung cancer to determine if a genetic deficiency of alpha1-antitrypsin (AAT) predisposes to lung cancer. The distributions of Pi types in these two groups of patients and healthy adults are similar. Serum TIC and AAT concentrations are elevated in lung cancer patients. However, patients with abnormal sputum cytology and no clinical lung cancer have normal levels of serum TIC and AAT. A genetic deficiency of AAT probably does not produce a state of increased susceptibility to the carcinogenic effects of respiratory carcinogens such as tobacco smoke.", "contents": "Serum alpha1-antitrypsin in patients with lung cancer or abnormal sputum cytology. Serum alpha1-antitrypsin Pi types and trypsin inhibitory capacity (TIC) were measured in 72 patients with lung cancer and in 196 patients with abnormal sputum cytology but no clinical evidence of lung cancer to determine if a genetic deficiency of alpha1-antitrypsin (AAT) predisposes to lung cancer. The distributions of Pi types in these two groups of patients and healthy adults are similar. Serum TIC and AAT concentrations are elevated in lung cancer patients. However, patients with abnormal sputum cytology and no clinical lung cancer have normal levels of serum TIC and AAT. A genetic deficiency of AAT probably does not produce a state of increased susceptibility to the carcinogenic effects of respiratory carcinogens such as tobacco smoke."}
{"id": "PMID:1086708", "title": "Comparison of multiple in vivo and in vitro parameters in untreated patients with Hodgkin's disease.", "content": "Multiple in vivo and in vitro immune parameters were used to examine 52 untreated patients with Hodgkin's disease in all stages. A significant number (p less than 0.01) of patients in all stages demonstrated abnormalities in DNCB sensitization, peripheral blood lymphocyte response to phytohemagglutination, absolute lymphocyte count, absolute number of T cells (as measured by spontaneous rosette formation with sheep erythrocytes), and absolute number of B cells (as measured by immunofluorescence with polyvalent antiserum). The number of T and B cells fell progressively with each stage, but the proportion of T to B cells remained constant. Cutaneous anergy was found in STAGES III and IV. Depressed circulating immunoglobulins were found in a few patients in all stages. Neither the total lymphocyte number nor the number of T and B cells correlated with the measures of lymphocyte function (skin test reactivity, DNCB sensitization, mitogen response, or immunoglobulin levels). This study suggests that selective abnormalities in the immune system exist even in early Hodgkin's disease, involving the absolute number of circulating T and B lymphocytes and T-lymphocyte function. The study fails, however, to define a consistent pattern of immune defects as characteristic of Hodgkin's Disease.", "contents": "Comparison of multiple in vivo and in vitro parameters in untreated patients with Hodgkin's disease. Multiple in vivo and in vitro immune parameters were used to examine 52 untreated patients with Hodgkin's disease in all stages. A significant number (p less than 0.01) of patients in all stages demonstrated abnormalities in DNCB sensitization, peripheral blood lymphocyte response to phytohemagglutination, absolute lymphocyte count, absolute number of T cells (as measured by spontaneous rosette formation with sheep erythrocytes), and absolute number of B cells (as measured by immunofluorescence with polyvalent antiserum). The number of T and B cells fell progressively with each stage, but the proportion of T to B cells remained constant. Cutaneous anergy was found in STAGES III and IV. Depressed circulating immunoglobulins were found in a few patients in all stages. Neither the total lymphocyte number nor the number of T and B cells correlated with the measures of lymphocyte function (skin test reactivity, DNCB sensitization, mitogen response, or immunoglobulin levels). This study suggests that selective abnormalities in the immune system exist even in early Hodgkin's disease, involving the absolute number of circulating T and B lymphocytes and T-lymphocyte function. The study fails, however, to define a consistent pattern of immune defects as characteristic of Hodgkin's Disease."}
{"id": "PMID:1086709", "title": "Cutaneous T-cell lymphoma.", "content": "A comparison of the detailed features and natural history of 12 patients with \"S\u00e9zary Syndrome\" with those in patients with classical \"mycosis fungoides\" has demonstrated that, with the exception of a leukemic phase in the former, the two syndromes are indistinguishable. Both disorders result from the proliferation of malignant thymus-derived lymphocytes with progressive involvement of skin, lymph nodes, and visceral organs; actual transition between these two syndromes have been documented. \"S\u00e9zary Syndrome\" and \"mycosis fungoides\" appear to represent variant clinical expressions of the same disease process. It is proposed that the present confusing and archaic terminology should be discarded and that the designation \"cutaneous T-cell lymphoma, with or without leukemic phase\" should be used in all future descriptions of this disorder.", "contents": "Cutaneous T-cell lymphoma. A comparison of the detailed features and natural history of 12 patients with \"S\u00e9zary Syndrome\" with those in patients with classical \"mycosis fungoides\" has demonstrated that, with the exception of a leukemic phase in the former, the two syndromes are indistinguishable. Both disorders result from the proliferation of malignant thymus-derived lymphocytes with progressive involvement of skin, lymph nodes, and visceral organs; actual transition between these two syndromes have been documented. \"S\u00e9zary Syndrome\" and \"mycosis fungoides\" appear to represent variant clinical expressions of the same disease process. It is proposed that the present confusing and archaic terminology should be discarded and that the designation \"cutaneous T-cell lymphoma, with or without leukemic phase\" should be used in all future descriptions of this disorder."}
{"id": "PMID:1086710", "title": "Radiation-related myocardial injury. Management of two cases.", "content": "Pericardial effusion and trifascicular block developed 5 years following mediastinal irradiation for Hodgkin's disease in a 19-year-old patient. Another 24-year-old patients had an acute myocardial infarction followed by severe angina pectoris 5 years following mediastinal irradiation for the same disease. A pericardial window and a permanent demand pacemaker were used in the first case; an aorto-coronary vein grafting was utilized in the second patient. Both patients responded to treatment and are well. Five other previously reported cases of myocardial injury that occurred 2 months to 8 years following mediastinal irradiation in young patients were reviewed. To our knowledge, successful surgical treatment of this disease entity has not been reported before. Close, long-term follow-up of patients who have received mediastinal irradiation should be helpful in the early recognition and successful management of these serious cardiac complications. The systematic clinical and radiographic surveilance of these patients should be supplemented by a routine 12-lead electrocardiogram.", "contents": "Radiation-related myocardial injury. Management of two cases. Pericardial effusion and trifascicular block developed 5 years following mediastinal irradiation for Hodgkin's disease in a 19-year-old patient. Another 24-year-old patients had an acute myocardial infarction followed by severe angina pectoris 5 years following mediastinal irradiation for the same disease. A pericardial window and a permanent demand pacemaker were used in the first case; an aorto-coronary vein grafting was utilized in the second patient. Both patients responded to treatment and are well. Five other previously reported cases of myocardial injury that occurred 2 months to 8 years following mediastinal irradiation in young patients were reviewed. To our knowledge, successful surgical treatment of this disease entity has not been reported before. Close, long-term follow-up of patients who have received mediastinal irradiation should be helpful in the early recognition and successful management of these serious cardiac complications. The systematic clinical and radiographic surveilance of these patients should be supplemented by a routine 12-lead electrocardiogram."}
{"id": "PMID:1086711", "title": "Hairy cell leukemia. An immunologic and ultrastructural study.", "content": "A case of hairy cell leukemia in a 39-year-old man is reported. Hairy cells from the peripheral blood, spleen, and bone marrow had lambda-type immunoglobulin on their surfaces; those from the peripheral blood and bone marrow also had IgD on their cell membranes. Frozen sections of spleen reacted with IgGEA, but not IgMEA or IgMEAC markers. Transmission electron microscopy revealed ribosome-lamella complexes in cells from the spleen, but not the peripheral blood. Scanning electron microscopy demonstrated a spectrum of cell surface morphology with many cells characterized by ridges and ruffles. The significance of these findings is considered and it is suggested that the hairy cell is a B lymphocyte.", "contents": "Hairy cell leukemia. An immunologic and ultrastructural study. A case of hairy cell leukemia in a 39-year-old man is reported. Hairy cells from the peripheral blood, spleen, and bone marrow had lambda-type immunoglobulin on their surfaces; those from the peripheral blood and bone marrow also had IgD on their cell membranes. Frozen sections of spleen reacted with IgGEA, but not IgMEA or IgMEAC markers. Transmission electron microscopy revealed ribosome-lamella complexes in cells from the spleen, but not the peripheral blood. Scanning electron microscopy demonstrated a spectrum of cell surface morphology with many cells characterized by ridges and ruffles. The significance of these findings is considered and it is suggested that the hairy cell is a B lymphocyte."}
{"id": "PMID:1086712", "title": "Nodular malignant lymphoma and amyloidosis. A case report.", "content": "A case of long-standing, nodular malignant lymphoma of lymphocytic type associated with nephrotic syndrome and systemic amyloidosis is presented. Review of literature revealed only six previously reported cases of systemic amyloidosis complicating non-Hodgkin's lymphomas. Recent studies have revealed that all of the nodular lymphomas are B-lymphocyte malignancies; that nodular lymphomas have high incidence (46-64%) of abnormality of intracellular immunoglobulin synthesis; that abnormality of serum immunoglobulin in such patients in uncommon (0-8%); and that at least one type of amyloid fibrils is made up of immunoglobulin light chains. These findings would suggest that there is an etiologic relationship between nodular lymphoma and systemic amyloidosis.", "contents": "Nodular malignant lymphoma and amyloidosis. A case report. A case of long-standing, nodular malignant lymphoma of lymphocytic type associated with nephrotic syndrome and systemic amyloidosis is presented. Review of literature revealed only six previously reported cases of systemic amyloidosis complicating non-Hodgkin's lymphomas. Recent studies have revealed that all of the nodular lymphomas are B-lymphocyte malignancies; that nodular lymphomas have high incidence (46-64%) of abnormality of intracellular immunoglobulin synthesis; that abnormality of serum immunoglobulin in such patients in uncommon (0-8%); and that at least one type of amyloid fibrils is made up of immunoglobulin light chains. These findings would suggest that there is an etiologic relationship between nodular lymphoma and systemic amyloidosis."}
{"id": "PMID:1086713", "title": "Convalescence following coronary surgery: a group experience.", "content": "The patient who has heart surgery and returns home will experience many problems in adjusting to a modified lifestyle. Successful rehabilitation depends on many factors-the knowledge the patient has about his condition, his own motivation and the support he receives from family and friends. If he has received good teaching in the hospital, his task will be much easier. But is in-hospital teaching enough? Current nursing research suggests that patients are more receptive to health teaching after they have returned home and benefit from sharing experiences with a group. The author describes the results of an investigation into the concerns of convalescent coronary patients and their wives and tells how group sessions helped ease their transition from hospital to a productive lifestyle.", "contents": "Convalescence following coronary surgery: a group experience. The patient who has heart surgery and returns home will experience many problems in adjusting to a modified lifestyle. Successful rehabilitation depends on many factors-the knowledge the patient has about his condition, his own motivation and the support he receives from family and friends. If he has received good teaching in the hospital, his task will be much easier. But is in-hospital teaching enough? Current nursing research suggests that patients are more receptive to health teaching after they have returned home and benefit from sharing experiences with a group. The author describes the results of an investigation into the concerns of convalescent coronary patients and their wives and tells how group sessions helped ease their transition from hospital to a productive lifestyle."}
{"id": "PMID:1086714", "title": "Methotrexate (NSC-740) with citrovorum factor (NSC-3590) rescue, alone and in combination with cyclophosphamide (NSC-26271), in ovarian cancer.", "content": "In a randomized study, 35 patients with advanced-stage epithelial ovarian cancers with progressive disease after prior single alkylating agent or combination-agent chemotherapy were treated with either intermediate high-dose methotrexate-citrovorum factor rescue alone or methotrexate-citrovorum factor rescue plus cyclophosphamide. Objective responses to both \"second-line\" chemotherapeutic regimens were observed. The objective response rate of 43% observed with the methotrexate-citrovorum factor plus cyclophosphamide treatment was superior to that achieved with methotrexate-citrovorum factor given alone. This combination of agents is the most effective second-line chemotherapeutic regimen for ovarian cancer that we have yet tested and we are therefore optimistic about its possibilities as first-line therapy. After early dosage modifications, serious toxic side effects were rare in this group of patients who had had extensive prior therapy.", "contents": "Methotrexate (NSC-740) with citrovorum factor (NSC-3590) rescue, alone and in combination with cyclophosphamide (NSC-26271), in ovarian cancer. In a randomized study, 35 patients with advanced-stage epithelial ovarian cancers with progressive disease after prior single alkylating agent or combination-agent chemotherapy were treated with either intermediate high-dose methotrexate-citrovorum factor rescue alone or methotrexate-citrovorum factor rescue plus cyclophosphamide. Objective responses to both \"second-line\" chemotherapeutic regimens were observed. The objective response rate of 43% observed with the methotrexate-citrovorum factor plus cyclophosphamide treatment was superior to that achieved with methotrexate-citrovorum factor given alone. This combination of agents is the most effective second-line chemotherapeutic regimen for ovarian cancer that we have yet tested and we are therefore optimistic about its possibilities as first-line therapy. After early dosage modifications, serious toxic side effects were rare in this group of patients who had had extensive prior therapy."}
{"id": "PMID:1086715", "title": "Experimental chemotherapy with 5-arylpyrimidine antifolates: preliminary studies on toxicity and responsiveness of sarcoma 180 to DDMP (NSC-19494) and DDMP with citrovorum factor (NSC-3590).", "content": "The 5-arylpyrimidine antifolate DDMP showed appreciable therapeutic activity against an ascitic form of Sarcoma 180 in BD2F1 mice. Antitumor effects were highly schedule and dose dependent at a limited number of doses within the range of 8--40 mg/kg. The best results (increased lifespan = 112%) were obtained with two doses of DDMP at 40 mg/kg given 4 days apart. The incorporation of citrovorum factor rescue in dose schedules with DDMP appeared to improve the therapeutic index. In multiple-dose schedules with citrovorum factor allowing an average of eight doses of DDMP at a maximum level of 16 mg/kg, increases in median lifespan were greater than 158% with a number of long-term survivors.", "contents": "Experimental chemotherapy with 5-arylpyrimidine antifolates: preliminary studies on toxicity and responsiveness of sarcoma 180 to DDMP (NSC-19494) and DDMP with citrovorum factor (NSC-3590). The 5-arylpyrimidine antifolate DDMP showed appreciable therapeutic activity against an ascitic form of Sarcoma 180 in BD2F1 mice. Antitumor effects were highly schedule and dose dependent at a limited number of doses within the range of 8--40 mg/kg. The best results (increased lifespan = 112%) were obtained with two doses of DDMP at 40 mg/kg given 4 days apart. The incorporation of citrovorum factor rescue in dose schedules with DDMP appeared to improve the therapeutic index. In multiple-dose schedules with citrovorum factor allowing an average of eight doses of DDMP at a maximum level of 16 mg/kg, increases in median lifespan were greater than 158% with a number of long-term survivors."}
{"id": "PMID:1086717", "title": "Innervation of skin glands in the frog.", "content": "A comparative study was undertaken on the innervation of mucous and granular glands in frog skin. Results obtained by the Falck-Hillarp fluorescence technique and cholinesterase staining indicated that both types of glands receive exclusively adrenergic innervation. Electron microscopy was used to investigate the innervation pattern at the ultrastructural level. The distribution of nerve terminals was found to differ in the two types of glands. In the mucous gland, terminals were found at a distance of about 0.5 mum from the basement membrane but never within the gland parenchyma. In the granular gland, the terminals were located between smooth muscle cells and also in direct contact with the secretory epithelium but never outside the basement membrane.", "contents": "Innervation of skin glands in the frog. A comparative study was undertaken on the innervation of mucous and granular glands in frog skin. Results obtained by the Falck-Hillarp fluorescence technique and cholinesterase staining indicated that both types of glands receive exclusively adrenergic innervation. Electron microscopy was used to investigate the innervation pattern at the ultrastructural level. The distribution of nerve terminals was found to differ in the two types of glands. In the mucous gland, terminals were found at a distance of about 0.5 mum from the basement membrane but never within the gland parenchyma. In the granular gland, the terminals were located between smooth muscle cells and also in direct contact with the secretory epithelium but never outside the basement membrane."}
{"id": "PMID:1086718", "title": "Apical secretion from taste bud and other epithelial cells in amphibians.", "content": "Taste buds of the axolotl, Ambystoma mexicanum, contain cells, previously undescribed in this species, which have a long apical process, and are similar to the Type III cells of mammalian taste buds, and to the gustatory cells in fish. In the supporting cells, there is evidence of periodic decapitation, in addition to secretion by exocytosis. Bilaminar fragments, which are leaf-shaped bodies formed of two dense laminae separated by a lucent gap, protrude from the apical microvilli of the supporting cells and are found detached in the extracellular secreted layer. Their form and dimensions suggest that they represent secreted lipo-protein material. Similar bilaminar fragments have been seen, in much smaller numbers, on some other epithelial cells in amphibians, and in fish. A unique case, in which rough endoplasmic reticulum was found in the extracellular layer of the axolotl oral epithelium, is reported; it had apparently been ejected from the cell. It is suggested that the axolotl produces a copious secretion at the taste bud pore, in order to wash the surface, and that the bilaminar fragments represent material aiding this cleansing process. The condition in the axolotl is compared with that in some other species, particularly Rana temporaria.", "contents": "Apical secretion from taste bud and other epithelial cells in amphibians. Taste buds of the axolotl, Ambystoma mexicanum, contain cells, previously undescribed in this species, which have a long apical process, and are similar to the Type III cells of mammalian taste buds, and to the gustatory cells in fish. In the supporting cells, there is evidence of periodic decapitation, in addition to secretion by exocytosis. Bilaminar fragments, which are leaf-shaped bodies formed of two dense laminae separated by a lucent gap, protrude from the apical microvilli of the supporting cells and are found detached in the extracellular secreted layer. Their form and dimensions suggest that they represent secreted lipo-protein material. Similar bilaminar fragments have been seen, in much smaller numbers, on some other epithelial cells in amphibians, and in fish. A unique case, in which rough endoplasmic reticulum was found in the extracellular layer of the axolotl oral epithelium, is reported; it had apparently been ejected from the cell. It is suggested that the axolotl produces a copious secretion at the taste bud pore, in order to wash the surface, and that the bilaminar fragments represent material aiding this cleansing process. The condition in the axolotl is compared with that in some other species, particularly Rana temporaria."}
{"id": "PMID:1086719", "title": "Permeability of the amphibian pars intermedia to peroxidase injected intravascularly.", "content": "To study the penetration of protein from the blood into and through the pars intermedia of anurans, the electron-dense tracer, horseradish peroxidase (HRP), was injected into the vascular system of the frog, Rana pipiens. Pinocytotic vesicles were seen along plasma membranes of the stellate cells abutting the pericapillary spaces between the pars intermedia and pars nervosa. The HRP was seen within both the pericapillary spaces and these pinocytotic vesicles. In addition, the HRP was very prominent in the extracellular spaces between adjacent stellate and/or MSH cells and around nerve endings and thus, appeared to move from the vascular spaces and into the interior of the pars intermedia. Therefore, the poorly vascularized pars intermedia may have such a route for transfer of materials throughout the gland.", "contents": "Permeability of the amphibian pars intermedia to peroxidase injected intravascularly. To study the penetration of protein from the blood into and through the pars intermedia of anurans, the electron-dense tracer, horseradish peroxidase (HRP), was injected into the vascular system of the frog, Rana pipiens. Pinocytotic vesicles were seen along plasma membranes of the stellate cells abutting the pericapillary spaces between the pars intermedia and pars nervosa. The HRP was seen within both the pericapillary spaces and these pinocytotic vesicles. In addition, the HRP was very prominent in the extracellular spaces between adjacent stellate and/or MSH cells and around nerve endings and thus, appeared to move from the vascular spaces and into the interior of the pars intermedia. Therefore, the poorly vascularized pars intermedia may have such a route for transfer of materials throughout the gland."}
{"id": "PMID:1086720", "title": "The inhibition by DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) of hnRNA and mRNA production in HeLa cells.", "content": "5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 75 muM) inhibited only 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis in HeLa cells, while the appearance of labeled cytoplasmic poly(A)-containing messenger RNA (mRNA) was reduced by approximately 95%. DRB-sensitive and resistant transcripts spanned the entire size range of hnRNA molecules. The relative poly(A) content of DRB-resistant hnRNA molecules was the same as that of normal hnRNA. In contrast to the effects of 3'deoxyadenosine, DRB appeared to inhibit the initiation of hnRNA chains, but did not directly interfere with labeling of poly(A). We present an interpretation of these results and propose how the results might be used to gain more information about nuclear RNA metabolism.", "contents": "The inhibition by DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) of hnRNA and mRNA production in HeLa cells. 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 75 muM) inhibited only 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis in HeLa cells, while the appearance of labeled cytoplasmic poly(A)-containing messenger RNA (mRNA) was reduced by approximately 95%. DRB-sensitive and resistant transcripts spanned the entire size range of hnRNA molecules. The relative poly(A) content of DRB-resistant hnRNA molecules was the same as that of normal hnRNA. In contrast to the effects of 3'deoxyadenosine, DRB appeared to inhibit the initiation of hnRNA chains, but did not directly interfere with labeling of poly(A). We present an interpretation of these results and propose how the results might be used to gain more information about nuclear RNA metabolism."}
{"id": "PMID:1086727", "title": "The relation between acute persisting spinal paralysis and poliomyelitis vaccine (oral): results of a WHO enquiry.", "content": "The present report presents the findings in 8 countries at the end of the first 5 years of an international investigation into the possible relationship between acute persisting spinal paralysis and the use of oral poliomyelitis vaccine.The most striking finding was the high association with type 3 virus in the recipient cases and with type 2 virus in the \"contacts\" and \"possible contacts\". Most of the cases in the recipient groups occurred in children under 5 years of age in all countries, but in the \"contact\" groups in the countries in which vaccination is offered through the year, many of the cases occurred in the non-immune parents of recently vaccinated infants.There were marked differences among countries, and it was not possible to pinpoint a single factor as the sole cause. However, the quality of the vaccine clearly played an important role. For some time, and certainly at the beginning of this enquiry, some of the countries were using vaccine from the same source without continuous external control and were using seed viruses at high passage levels. The situation changed during the enquiry and the incidence of paralytic cases decreased. The enquiry will be continued and particular efforts will be made to establish the cause of the associated paralysis. The findings of the enquiry confirm that oral Sabin poliomyelitis vaccines are among the safest vaccines in use today.", "contents": "The relation between acute persisting spinal paralysis and poliomyelitis vaccine (oral): results of a WHO enquiry. The present report presents the findings in 8 countries at the end of the first 5 years of an international investigation into the possible relationship between acute persisting spinal paralysis and the use of oral poliomyelitis vaccine.The most striking finding was the high association with type 3 virus in the recipient cases and with type 2 virus in the \"contacts\" and \"possible contacts\". Most of the cases in the recipient groups occurred in children under 5 years of age in all countries, but in the \"contact\" groups in the countries in which vaccination is offered through the year, many of the cases occurred in the non-immune parents of recently vaccinated infants.There were marked differences among countries, and it was not possible to pinpoint a single factor as the sole cause. However, the quality of the vaccine clearly played an important role. For some time, and certainly at the beginning of this enquiry, some of the countries were using vaccine from the same source without continuous external control and were using seed viruses at high passage levels. The situation changed during the enquiry and the incidence of paralytic cases decreased. The enquiry will be continued and particular efforts will be made to establish the cause of the associated paralysis. The findings of the enquiry confirm that oral Sabin poliomyelitis vaccines are among the safest vaccines in use today."}
{"id": "PMID:1086728", "title": "Trial of a reduced dose of measles vaccine in Nigerian children.", "content": "To evaluate the effectiveness of measles vaccine in reduced dosage, Schwarz live, further attenuated measles virus vaccine was given in various doses by jet injector to 967 seronegative children aged 7-30 months in rural villages in the Northwest State of Nigeria. For children over 12 months of age, reducing vaccine virus dosage by 40%, 60%, or 80% led to progressive and statistically significant reductions in the seroconversion rates from 89% in children receiving the full dose to a minimum value of 46%. Reducing the volume of the inoculum below the standard of 0.5 ml resulted in a further reduction of seroconversion rates at each antigen dose level. Seroconversion rates in all dosage groups were lower for children below 12 months of age. These data suggest that dose reduction would not be a satisfactory economy measure in measles vaccination campaigns.", "contents": "Trial of a reduced dose of measles vaccine in Nigerian children. To evaluate the effectiveness of measles vaccine in reduced dosage, Schwarz live, further attenuated measles virus vaccine was given in various doses by jet injector to 967 seronegative children aged 7-30 months in rural villages in the Northwest State of Nigeria. For children over 12 months of age, reducing vaccine virus dosage by 40%, 60%, or 80% led to progressive and statistically significant reductions in the seroconversion rates from 89% in children receiving the full dose to a minimum value of 46%. Reducing the volume of the inoculum below the standard of 0.5 ml resulted in a further reduction of seroconversion rates at each antigen dose level. Seroconversion rates in all dosage groups were lower for children below 12 months of age. These data suggest that dose reduction would not be a satisfactory economy measure in measles vaccination campaigns."}
{"id": "PMID:1086729", "title": "Kaeng Khoi virus from naturally infected bedbugs (cimicidae) and immature free-tailed bats.", "content": "Kaeng Khoi virus was recovered from bedbugs (Stricticimex parvus and Cimex insuetus) and from suckling wrinkle-lipped bats (Tadarida plicata) collected in central Thailand. The data implicate bedbugs as possible vectors of this virus.", "contents": "Kaeng Khoi virus from naturally infected bedbugs (cimicidae) and immature free-tailed bats. Kaeng Khoi virus was recovered from bedbugs (Stricticimex parvus and Cimex insuetus) and from suckling wrinkle-lipped bats (Tadarida plicata) collected in central Thailand. The data implicate bedbugs as possible vectors of this virus."}
{"id": "PMID:1086731", "title": "Effect of cysteine on the hepatic toxicity and antischistosomal activity of antimonyl potassium tartrate.", "content": "Cysteine produced a significant and progressive reduction in the toxicity of antimonyl potassium tartrate (APT) when the two substances were injected into mice and rabbits in ratios of APT to cysteine ranging from 1:1 to 1:3. The reduction in toxicity was highest with the 1:3 ratio. However, the combination of the two substances, especially in the ratio of 1:3, appreciably reduced the antischistosomal activity of APT both in vivo and in vitro.", "contents": "Effect of cysteine on the hepatic toxicity and antischistosomal activity of antimonyl potassium tartrate. Cysteine produced a significant and progressive reduction in the toxicity of antimonyl potassium tartrate (APT) when the two substances were injected into mice and rabbits in ratios of APT to cysteine ranging from 1:1 to 1:3. The reduction in toxicity was highest with the 1:3 ratio. However, the combination of the two substances, especially in the ratio of 1:3, appreciably reduced the antischistosomal activity of APT both in vivo and in vitro."}
{"id": "PMID:1086732", "title": "The suppression, by Helisoma duryi, of the cercarial production of Schistosoma mansoni-infected Biomphalaria pfeifferi.", "content": "Biological control of the intermediate hosts of Schistosoma mansoni and S. haematobium by means of a competitor snail, Helisoma duryi, has been suggested. In the present laboratory study, the influence of H. duryi on the relationship between the parasite and the intermediate host was investigated. The results indicated that H. duryi behaves as a \"decoy\" if it is present when Biomphalaria pfeifferi is exposed to the miracidia of S. mansoni, and that the continued presence of H. duryi in the aquarium after B. pfeifferi has been exposed greatly reduces cercarial production. When these two species were present in equal numbers, cercarial production was reduced by 95.9% in comparison with a control group.", "contents": "The suppression, by Helisoma duryi, of the cercarial production of Schistosoma mansoni-infected Biomphalaria pfeifferi. Biological control of the intermediate hosts of Schistosoma mansoni and S. haematobium by means of a competitor snail, Helisoma duryi, has been suggested. In the present laboratory study, the influence of H. duryi on the relationship between the parasite and the intermediate host was investigated. The results indicated that H. duryi behaves as a \"decoy\" if it is present when Biomphalaria pfeifferi is exposed to the miracidia of S. mansoni, and that the continued presence of H. duryi in the aquarium after B. pfeifferi has been exposed greatly reduces cercarial production. When these two species were present in equal numbers, cercarial production was reduced by 95.9% in comparison with a control group."}
{"id": "PMID:1086733", "title": "Parasite cultivation in relation to research on the chemotherapy of malaria.", "content": "Attempts to develop techniques for the continuous in vitro cultivation of the malaria parasite have not yet proved successful. It has not been possible to obtain the complete sporogonic development of the parasite in vitro although some progress was made with Plasmodium relictum and P. berghei. Exoerythrocytic stages of P. gallinaceum have been successfully cultivated in vitro in tissue explants and those of P. fallax have been grown in turkey primary embryo tissue cells. With the recent development of mammalian liver cell lines, prospects for the in vitro cultivation of exoerythrocytic stages of mammalian plasmodia are greatly improved. While it is still not possible to cultivate erythrocytic stages of plasmodia serially in vitro some species have been successfully grown through one asexual cycle. This progress has led to a number of applications of parasite cultivation to chemotherapeutic studies, to the testing of new antimalarial drugs, and especially to the testing of the susceptibility of P. falciparum to chloroquine. Cultivation technique is greatly improved by an appropriate choice of culture media. The addition of fresh red cells to the subculture system permits relatively high rates of invasion and multiplication of the parasite to be obtained. As well as its application in the screening and evaluation of antimalarial compounds, the in vitro cultivation technique is also very suitable for studying the entry mechanism of the parasite into red blood cells.", "contents": "Parasite cultivation in relation to research on the chemotherapy of malaria. Attempts to develop techniques for the continuous in vitro cultivation of the malaria parasite have not yet proved successful. It has not been possible to obtain the complete sporogonic development of the parasite in vitro although some progress was made with Plasmodium relictum and P. berghei. Exoerythrocytic stages of P. gallinaceum have been successfully cultivated in vitro in tissue explants and those of P. fallax have been grown in turkey primary embryo tissue cells. With the recent development of mammalian liver cell lines, prospects for the in vitro cultivation of exoerythrocytic stages of mammalian plasmodia are greatly improved. While it is still not possible to cultivate erythrocytic stages of plasmodia serially in vitro some species have been successfully grown through one asexual cycle. This progress has led to a number of applications of parasite cultivation to chemotherapeutic studies, to the testing of new antimalarial drugs, and especially to the testing of the susceptibility of P. falciparum to chloroquine. Cultivation technique is greatly improved by an appropriate choice of culture media. The addition of fresh red cells to the subculture system permits relatively high rates of invasion and multiplication of the parasite to be obtained. As well as its application in the screening and evaluation of antimalarial compounds, the in vitro cultivation technique is also very suitable for studying the entry mechanism of the parasite into red blood cells."}
{"id": "PMID:1086734", "title": "Antibody persistence after surgical treatment of echinococcosis.", "content": "Studies on the persistence and levels of antibodies in 280 patients at different intervals following surgery for echinococcosis are described. Post-operative antibody levels were investigated by means of four immunodiagnostic methods, namely, the complement fixation, latex agglutination, indirect haemagglutination, and intradermal tests. After surgical removal of the cysts the tests became negative in some patients, whereas in others they remained positive for many years. As the post-operative period increased, the percentage of positive results fell and this fall was faster in lung echinococcosis than in liver echinococcosis. The titres obtained with the serological tests decreased at the same rate as the percentage of positive results, but the intradermal test remained positive, with an unchanged titre, for many years. In the case of secondary echinococcosis, medium and high titres predominated in all the tests. These immunological investigations during the post-operative period make it possible to evaluate the results of surgery and, to some extent, to clarify the prognosis.", "contents": "Antibody persistence after surgical treatment of echinococcosis. Studies on the persistence and levels of antibodies in 280 patients at different intervals following surgery for echinococcosis are described. Post-operative antibody levels were investigated by means of four immunodiagnostic methods, namely, the complement fixation, latex agglutination, indirect haemagglutination, and intradermal tests. After surgical removal of the cysts the tests became negative in some patients, whereas in others they remained positive for many years. As the post-operative period increased, the percentage of positive results fell and this fall was faster in lung echinococcosis than in liver echinococcosis. The titres obtained with the serological tests decreased at the same rate as the percentage of positive results, but the intradermal test remained positive, with an unchanged titre, for many years. In the case of secondary echinococcosis, medium and high titres predominated in all the tests. These immunological investigations during the post-operative period make it possible to evaluate the results of surgery and, to some extent, to clarify the prognosis."}
{"id": "PMID:1086735", "title": "Experimental chemotherapy in leprosy.", "content": "The Memorandum reviews the considerable progress that has been made in research on the chemotherapy of leprosy during the last 10-15 years, as a result of which it is now possible to study the same topics in leprosy as are studied in other bacterial diseases. Thus drugs have been screened in mice for their activity against Mycobacterium leprae. Those that have been found to have the greatest activity against M. leprae at acceptable dosages-dapsone, rifampicin, and clofazimine-have been characterized in terms of the minimal effective dosage and rate of bacterial kill. Similarly, their pharmacokinetics in man and in certain animals have been defined. The theoretical basis for drug trials in leprosy patients is discussed in terms of the number of viable and the number of dead M. leprae that remain at various stages of therapy.", "contents": "Experimental chemotherapy in leprosy. The Memorandum reviews the considerable progress that has been made in research on the chemotherapy of leprosy during the last 10-15 years, as a result of which it is now possible to study the same topics in leprosy as are studied in other bacterial diseases. Thus drugs have been screened in mice for their activity against Mycobacterium leprae. Those that have been found to have the greatest activity against M. leprae at acceptable dosages-dapsone, rifampicin, and clofazimine-have been characterized in terms of the minimal effective dosage and rate of bacterial kill. Similarly, their pharmacokinetics in man and in certain animals have been defined. The theoretical basis for drug trials in leprosy patients is discussed in terms of the number of viable and the number of dead M. leprae that remain at various stages of therapy."}
{"id": "PMID:1086736", "title": "Tuberculin sensitivity in guinea-pigs after vaccination with varying doses of BCG of 12 different strains.", "content": "In several previously reported studies a number of BCG strains, including those most widely used in vaccine production, were ranked according to their in vivo activity in various experimental models in rodents and to the local and allergic response that they provoked in children. In this report, 12 strains are ranked in terms of tuberculin conversion in guinea-pigs according to the minimum sensitizing dose. For 10 of these strains, this minimum dose is very low, ranging from 5 to 50 culturable particles. Thus the traditional practice of tuberculin-testing guinea-pigs that have been vaccinated with a full human dose of BCG (of the order of one million culturable particles) has such a low discriminating power that it is useless as a routine test for currently used vaccines. The ranking obtained in this model was largely but not completely in accordance with similar rankings in other models. The Tokyo strain and, to some extent, the London strain ranked comparatively lower than they do in children. The pronounced lack of virulence of the Prague strain was confirmed.", "contents": "Tuberculin sensitivity in guinea-pigs after vaccination with varying doses of BCG of 12 different strains. In several previously reported studies a number of BCG strains, including those most widely used in vaccine production, were ranked according to their in vivo activity in various experimental models in rodents and to the local and allergic response that they provoked in children. In this report, 12 strains are ranked in terms of tuberculin conversion in guinea-pigs according to the minimum sensitizing dose. For 10 of these strains, this minimum dose is very low, ranging from 5 to 50 culturable particles. Thus the traditional practice of tuberculin-testing guinea-pigs that have been vaccinated with a full human dose of BCG (of the order of one million culturable particles) has such a low discriminating power that it is useless as a routine test for currently used vaccines. The ranking obtained in this model was largely but not completely in accordance with similar rankings in other models. The Tokyo strain and, to some extent, the London strain ranked comparatively lower than they do in children. The pronounced lack of virulence of the Prague strain was confirmed."}
{"id": "PMID:1086738", "title": "Diagnosis of rheumatism by means of universal criteria, obtained by the cybernetic method.", "content": "With a view to establishing standardized criteria for the diagnosis of rheumatism suitable for widespread use in polyclinics and in large-scale epidemiological investigations, 3000 adults and children with rheumatism or one of seven similar diseases were subjected to multiple screening in eight centres in the USSR using a specially prepared standard card and standard methods. The information obtained was processed by cybernetic methods. Following determination of the sensitivity, specificity, and informational value of 486 signs of rheumatism, 68 of the most indicative signs were selected as criteria of rheumatism. These were included in the final table and weighted according to their indicational value both individually and in groups as syndromes. Using the table it is possible by simple calculation, without using a computer, to arrive at an objective evaluation of the reliability of the clinical diagnosis of rheumatism in patients with one of a group of eight similar diseases. The final diagnostic table was tested on 562 patients selected epidemiologically or from polyclinics or clinics and was shown to result in a high degree of diagnostic precision (higher than 95%).", "contents": "Diagnosis of rheumatism by means of universal criteria, obtained by the cybernetic method. With a view to establishing standardized criteria for the diagnosis of rheumatism suitable for widespread use in polyclinics and in large-scale epidemiological investigations, 3000 adults and children with rheumatism or one of seven similar diseases were subjected to multiple screening in eight centres in the USSR using a specially prepared standard card and standard methods. The information obtained was processed by cybernetic methods. Following determination of the sensitivity, specificity, and informational value of 486 signs of rheumatism, 68 of the most indicative signs were selected as criteria of rheumatism. These were included in the final table and weighted according to their indicational value both individually and in groups as syndromes. Using the table it is possible by simple calculation, without using a computer, to arrive at an objective evaluation of the reliability of the clinical diagnosis of rheumatism in patients with one of a group of eight similar diseases. The final diagnostic table was tested on 562 patients selected epidemiologically or from polyclinics or clinics and was shown to result in a high degree of diagnostic precision (higher than 95%)."}
{"id": "PMID:1086739", "title": "Sudan: national health programme and primary health care, 1977/78-1983/84.", "content": "As a follow-up to the national health programming process developed in 1975 in Sudan, a primary health care programme for the whole country was formulated with assistance from WHO. In this article the methods used in the programming and formulation are described and discussed. These methods ensured an intersectoral approach on which technical, cultural, socioeconomic, financial, and political considerations were based. Areas in the field of health and rural development requiring government and community action during the period 1977/78-1983/84 are identified. Details on the strategies for population coverage of rural and nomadic communities with primary health care are given. Fundamental to these strategies is community participation in the development of primary health care within community development as a whole.The guiding principles of these strategies are their technical, political, social and financial feasibility. The social relevance of the primary health care programme for the community and the developmental sectors is emphasized.", "contents": "Sudan: national health programme and primary health care, 1977/78-1983/84. As a follow-up to the national health programming process developed in 1975 in Sudan, a primary health care programme for the whole country was formulated with assistance from WHO. In this article the methods used in the programming and formulation are described and discussed. These methods ensured an intersectoral approach on which technical, cultural, socioeconomic, financial, and political considerations were based. Areas in the field of health and rural development requiring government and community action during the period 1977/78-1983/84 are identified. Details on the strategies for population coverage of rural and nomadic communities with primary health care are given. Fundamental to these strategies is community participation in the development of primary health care within community development as a whole.The guiding principles of these strategies are their technical, political, social and financial feasibility. The social relevance of the primary health care programme for the community and the developmental sectors is emphasized."}
{"id": "PMID:1086740", "title": "Epidemiological studies on onchocerciasis by means of a new field technique.", "content": "A new membrane filter concentration technique for the detection and quantification of Onchocerca volvulus microfilariae in skin snips was compared for sensitivity and efficiency with a widely used \"standard\" technique. A field study was carried out in five villages in an onchocerciasis focus north-east of the town of Sokod\u00e9, M\u00f4 river valley, Togo. Use of the new technique resulted in a substantial rise in the observed prevalence and density of microfilariae.", "contents": "Epidemiological studies on onchocerciasis by means of a new field technique. A new membrane filter concentration technique for the detection and quantification of Onchocerca volvulus microfilariae in skin snips was compared for sensitivity and efficiency with a widely used \"standard\" technique. A field study was carried out in five villages in an onchocerciasis focus north-east of the town of Sokod\u00e9, M\u00f4 river valley, Togo. Use of the new technique resulted in a substantial rise in the observed prevalence and density of microfilariae."}
{"id": "PMID:1086741", "title": "Socioeconomic scoring in an urban area of a developing country.", "content": "A group of variables that might define \"life-style\" was selected and a questionnaire based on these was distributed to the families of 2350 schoolchildren in Accra, Ghana to determine the presence or absence of these variables. Analysis of the replies revealed a set of answers that formed a related group. A scoring system was developed in which rarer attributes were weighted, and the community was thus graded by socioeconomic status on a ten-point scale. Principles for developing questionnaires applicable to other types of community were defined.", "contents": "Socioeconomic scoring in an urban area of a developing country. A group of variables that might define \"life-style\" was selected and a questionnaire based on these was distributed to the families of 2350 schoolchildren in Accra, Ghana to determine the presence or absence of these variables. Analysis of the replies revealed a set of answers that formed a related group. A scoring system was developed in which rarer attributes were weighted, and the community was thus graded by socioeconomic status on a ten-point scale. Principles for developing questionnaires applicable to other types of community were defined."}
{"id": "PMID:1086742", "title": "A streptomycin-sensitive revertant of a streptomycin-dependent strain of Vibrio cholerae.", "content": "A study was made of a streptomycin-sensitive revertant of a streptomycin-dependent strain of V. cholerae. The revertant, designated as strain KB599, appears to be avirulent in animal experimental models. However, no evidence of multiplication was found in infant mice.", "contents": "A streptomycin-sensitive revertant of a streptomycin-dependent strain of Vibrio cholerae. A study was made of a streptomycin-sensitive revertant of a streptomycin-dependent strain of V. cholerae. The revertant, designated as strain KB599, appears to be avirulent in animal experimental models. However, no evidence of multiplication was found in infant mice."}
{"id": "PMID:1086743", "title": "Determination of improved myocardial perfusion after aortocoronary bypass surgery by exercise rubidium-81 scintigraphy.", "content": "Practical and noninvasive means are needed for evaluating efficacy of coronary bypass surgery (CBS) in improving blood flow (CBF) to ischemic myocardium in coronary patients. Revascularization was assessed in 15 patients by pre- and post-CBS rest and exercise rubidium-81 myocardial images with a scintillation camera equipped with pinhole collimator and high-energy shield. Ischemic areas were detected by decreased 81Rb activity after exercise compared to rest. Before CBS all patients had exercise angina (EA), positive treadmill ECG (TECG), and abnormal exercise 81Rb scans. After CBS all 15 patients had increased physical activity before angina or completion of treadmill exercise with increased heart rate-blood pressure product (HRBP) (+ 63 +/- 3.2 X 10(2) bpm - mm Hg) in 14 of 15 patients indicating increased CBF; four had positive TECG, and five had EA. The increased HRBP in 14 patients was associated with improved post-CBS exercise 81Rb scans: six had normal patterns while nine were improved with less ischemic patterns. Further, lack of angina and increased exercise tolerance correlated closely with increased 81Rb myocardial perfusion. Thus pre- and postoperative rest and exercise 81Rb scintigraphy gives an accurate, noninvasive, objective approach for evaluation of CBF following CBS and demonstrates the usefulness of this revascularization procedure in coronary patients.", "contents": "Determination of improved myocardial perfusion after aortocoronary bypass surgery by exercise rubidium-81 scintigraphy. Practical and noninvasive means are needed for evaluating efficacy of coronary bypass surgery (CBS) in improving blood flow (CBF) to ischemic myocardium in coronary patients. Revascularization was assessed in 15 patients by pre- and post-CBS rest and exercise rubidium-81 myocardial images with a scintillation camera equipped with pinhole collimator and high-energy shield. Ischemic areas were detected by decreased 81Rb activity after exercise compared to rest. Before CBS all patients had exercise angina (EA), positive treadmill ECG (TECG), and abnormal exercise 81Rb scans. After CBS all 15 patients had increased physical activity before angina or completion of treadmill exercise with increased heart rate-blood pressure product (HRBP) (+ 63 +/- 3.2 X 10(2) bpm - mm Hg) in 14 of 15 patients indicating increased CBF; four had positive TECG, and five had EA. The increased HRBP in 14 patients was associated with improved post-CBS exercise 81Rb scans: six had normal patterns while nine were improved with less ischemic patterns. Further, lack of angina and increased exercise tolerance correlated closely with increased 81Rb myocardial perfusion. Thus pre- and postoperative rest and exercise 81Rb scintigraphy gives an accurate, noninvasive, objective approach for evaluation of CBF following CBS and demonstrates the usefulness of this revascularization procedure in coronary patients."}
{"id": "PMID:1086744", "title": "Long-term follow-up of patients with coronary artery bypass grafts.", "content": "A follow-up study of 1532 patients who had coronary artery bypass grafts from 1969 through 1974 revealed that the 5-year survival rate was substantially better than that recorded in previously published series of patients with similar disease demonstrated angiographically who did not undergo operation. Within this group of operated patients, those who had complete myocardial revascularization (i.e., three grafts for those with three-vessel disease) experienced a significantly better long-term prognosis than those who had fewer grafts than the number of vessels obstructed. The mortality curve in the completely revascularized patients was very similar to that of the general population of the United States, corrected to correspond to this patient sample in age and sex.", "contents": "Long-term follow-up of patients with coronary artery bypass grafts. A follow-up study of 1532 patients who had coronary artery bypass grafts from 1969 through 1974 revealed that the 5-year survival rate was substantially better than that recorded in previously published series of patients with similar disease demonstrated angiographically who did not undergo operation. Within this group of operated patients, those who had complete myocardial revascularization (i.e., three grafts for those with three-vessel disease) experienced a significantly better long-term prognosis than those who had fewer grafts than the number of vessels obstructed. The mortality curve in the completely revascularized patients was very similar to that of the general population of the United States, corrected to correspond to this patient sample in age and sex."}
{"id": "PMID:1086745", "title": "Refractory angina pectoris: follow-up after intraaortic balloon pumping and surgery.", "content": "Ffity-five patients with recurrent severe angina pectoris at rest that was resistant to medical therapy were treated with intraaortic balloon pumping (IABP), angiography, and vein bypass surgery. There were 25 patients with typical angina with ST depression during pain, 12 with Prinzmetal's angina, and 18 patients with angina in the early recovery phase following \"transmural\" myocardial infarction. The severity and frequency of ischemic attacks were documented with hemodynamic and continuous electrocardiographic monitoring. A marked reduction in both frequency and intensity of attacks was produced by IABP. Temporary cessation of IABP resulted in rapid recurrence of angina in 40% of patients. All underwent selective coronary angiography and revascularization surgery. The overall mortality was 5.5% and the incidence of intraoperative myocardial infarction was under 2%. Follow-up evaluation after an average of 18 months has shown no late deaths and sustained clinical improvement.", "contents": "Refractory angina pectoris: follow-up after intraaortic balloon pumping and surgery. Ffity-five patients with recurrent severe angina pectoris at rest that was resistant to medical therapy were treated with intraaortic balloon pumping (IABP), angiography, and vein bypass surgery. There were 25 patients with typical angina with ST depression during pain, 12 with Prinzmetal's angina, and 18 patients with angina in the early recovery phase following \"transmural\" myocardial infarction. The severity and frequency of ischemic attacks were documented with hemodynamic and continuous electrocardiographic monitoring. A marked reduction in both frequency and intensity of attacks was produced by IABP. Temporary cessation of IABP resulted in rapid recurrence of angina in 40% of patients. All underwent selective coronary angiography and revascularization surgery. The overall mortality was 5.5% and the incidence of intraoperative myocardial infarction was under 2%. Follow-up evaluation after an average of 18 months has shown no late deaths and sustained clinical improvement."}
{"id": "PMID:1086746", "title": "Patency of internal mammary-coronary grafts.", "content": "Patency of internal mammary artery (IMA) coronary grafts was evaluated in 150 patients having an early (20 days) and late (13 months) postoperative angiogram. Early and late failure for 76 right IMA grafts was 3 and 5 respectively and for 139 left IMA grafts 7 and 7 an overall IMA patency of 95% and 90%. The IMA and coronary internal diameter did not relate to graft closure. Right IMA free flow did not correlate with graft closure. Free flow for thrombosed left IMA grafts (91 ml/min) was significantly (P less than 0.05) lower than for patent grafts (118 ml/min). Right IMA anastomotic flow of 55 ml/min was significantly (P less than 0.001) higher than occluded graft flow of 26 ml/min as was true for patent left IMA grafts (55 ml/min) and occluded grafts (43 ml/min) (P less than 0.025). Five of 215 IMA grafts had diffuse stenosis (greater than 50%). Saphenous vein coronary bypass was performed in 88 instances with an early patency of 89% (79/88) and a late patency of 88% (78/88). Only one of 38 vein grafts to the right coronary artery failed whereas eight of 44 grafts to the circumflex artery occluded. Experience was associated with increased late IMA patency from 81% (57/70), to 89% (;8/76), to 99% (68/69) in each successive group of 50 patients. Currently, the left IMA is routinely used for the left anterior descending artery and the right IMA is rarely used.", "contents": "Patency of internal mammary-coronary grafts. Patency of internal mammary artery (IMA) coronary grafts was evaluated in 150 patients having an early (20 days) and late (13 months) postoperative angiogram. Early and late failure for 76 right IMA grafts was 3 and 5 respectively and for 139 left IMA grafts 7 and 7 an overall IMA patency of 95% and 90%. The IMA and coronary internal diameter did not relate to graft closure. Right IMA free flow did not correlate with graft closure. Free flow for thrombosed left IMA grafts (91 ml/min) was significantly (P less than 0.05) lower than for patent grafts (118 ml/min). Right IMA anastomotic flow of 55 ml/min was significantly (P less than 0.001) higher than occluded graft flow of 26 ml/min as was true for patent left IMA grafts (55 ml/min) and occluded grafts (43 ml/min) (P less than 0.025). Five of 215 IMA grafts had diffuse stenosis (greater than 50%). Saphenous vein coronary bypass was performed in 88 instances with an early patency of 89% (79/88) and a late patency of 88% (78/88). Only one of 38 vein grafts to the right coronary artery failed whereas eight of 44 grafts to the circumflex artery occluded. Experience was associated with increased late IMA patency from 81% (57/70), to 89% (;8/76), to 99% (68/69) in each successive group of 50 patients. Currently, the left IMA is routinely used for the left anterior descending artery and the right IMA is rarely used."}
{"id": "PMID:1086747", "title": "Balloon counterpulsation following surgery for ischemic heart disease.", "content": "Intraaortic balloon counterpulsation (IABC) was used in 40 patients who developed left ventricular pump failure following surgery for ischemic heart disease. The IABC was inserted in the operating room in 32 patients, and within 36 hours of surgery in eight patients. Four patients could not be weaned from cardiopulmonary bypass and died in the operating room. Of the other 36 patients, eight died while on IABC. Twenty-eight patients (70%) were successfully weaned from IABC, although six died later in hospital. Twenty-two patients (55%) survived hospitalization. No late deaths have occurred at 5-33 months of follow-up (mean 16-4 months). Twenty (91%) of 22 survivors are Class I or II at time of this report. Postoperative pump failure occurs because of perioperative acute myocardial infarction and/or major preoperative left ventricular dysfunction. Patients with perioperative infarction who have no prior left ventricular dysfunction have a significantly lower mortality (14%) than do those patients with preoperative left ventricular dysfunction who have subsequent acute perioperative infarction (mortality 67%) P less than 0.05.", "contents": "Balloon counterpulsation following surgery for ischemic heart disease. Intraaortic balloon counterpulsation (IABC) was used in 40 patients who developed left ventricular pump failure following surgery for ischemic heart disease. The IABC was inserted in the operating room in 32 patients, and within 36 hours of surgery in eight patients. Four patients could not be weaned from cardiopulmonary bypass and died in the operating room. Of the other 36 patients, eight died while on IABC. Twenty-eight patients (70%) were successfully weaned from IABC, although six died later in hospital. Twenty-two patients (55%) survived hospitalization. No late deaths have occurred at 5-33 months of follow-up (mean 16-4 months). Twenty (91%) of 22 survivors are Class I or II at time of this report. Postoperative pump failure occurs because of perioperative acute myocardial infarction and/or major preoperative left ventricular dysfunction. Patients with perioperative infarction who have no prior left ventricular dysfunction have a significantly lower mortality (14%) than do those patients with preoperative left ventricular dysfunction who have subsequent acute perioperative infarction (mortality 67%) P less than 0.05."}
{"id": "PMID:1086748", "title": "Survey of amniocentesis for fetal sex determination in hemophilia carriers.", "content": "A study was designed to determine whether there is an increased risk of complications when amniocentesis for fetal sex determination is performed on hemophilia carriers. Questionnaires were sent to 112 medical centers providing this service in the United States, and to 19 outside the United States. Responses were received from 76% of the centers in the United States. Data on 11,819 taps were obtained. Only 75 taps (0.64%) were performed for the indication of hemophilia. The frequency of fetal deaths in the general sample (1.84%) was not significantly different from that in the subsample of hemophilia carriers (1.33%). The results of this survey correspond very closely to data from a National Registry on amniocentesis for various indicaions in such variables as the number of taps needed for diagnosis, color of the fluid obtained, and number of dry taps. Carrier women who had bleeding problems during the monitored pregnancy are described. The problems might have been related to the amniocentesis in three women. It is calculated that only 2-4% of hemophilia carrier women who might have amniocentesis are utilizing the service.", "contents": "Survey of amniocentesis for fetal sex determination in hemophilia carriers. A study was designed to determine whether there is an increased risk of complications when amniocentesis for fetal sex determination is performed on hemophilia carriers. Questionnaires were sent to 112 medical centers providing this service in the United States, and to 19 outside the United States. Responses were received from 76% of the centers in the United States. Data on 11,819 taps were obtained. Only 75 taps (0.64%) were performed for the indication of hemophilia. The frequency of fetal deaths in the general sample (1.84%) was not significantly different from that in the subsample of hemophilia carriers (1.33%). The results of this survey correspond very closely to data from a National Registry on amniocentesis for various indicaions in such variables as the number of taps needed for diagnosis, color of the fluid obtained, and number of dry taps. Carrier women who had bleeding problems during the monitored pregnancy are described. The problems might have been related to the amniocentesis in three women. It is calculated that only 2-4% of hemophilia carrier women who might have amniocentesis are utilizing the service."}
{"id": "PMID:1086749", "title": "T, B and K cells in autoimmune thyroid disease.", "content": "The K-cell cytotoxic activity of peripheral blood lymphoid cells from 104 patients with autoimmune thyroid disease and from age and sex matched control subjects was measured using chicken erythrocytes as target cells. Patients with Hashimoto thyroiditis,primary hypothyroidism and thyrotoxicosis who were either newly diagnosed and untreated or had received therapy for less than or equal to 1 year showed a significant increase in K-cell cytotoxic activity. Patients who had received treatment for greater than 1 year and less than or equal to 5 years showed no such comparable increase in cytotoxic activity. Within the group of patients with untreated thyrotoxicosis it was found that K-cell cytotoxic activity was related to both goitre size and serum antibody titre. Thus patients with little or no goitre showed a highly significant elevation of cytotoxic activity whereas patients with moderate to large goitres gave values within the normal range. Similarly patients with no detectable serum thyroid autoantibodies showed high K-cell activity while patients with positive antibody titres did not. It was also shown that neither the absolute number nor the proportion of circulating T and B lymphocytes in patients with autoimmune thyroid disease as assessed by the sheep red cell rosette method and by indirect immunofluorescence was significantly different from that observed in the normal control population. No correlation was found between peripheral blood K-cell cytotoxic activity and the percentage of circulating null cells, i.e. 100-(percentage T + percentage B) in either patients or control subjects.", "contents": "T, B and K cells in autoimmune thyroid disease. The K-cell cytotoxic activity of peripheral blood lymphoid cells from 104 patients with autoimmune thyroid disease and from age and sex matched control subjects was measured using chicken erythrocytes as target cells. Patients with Hashimoto thyroiditis,primary hypothyroidism and thyrotoxicosis who were either newly diagnosed and untreated or had received therapy for less than or equal to 1 year showed a significant increase in K-cell cytotoxic activity. Patients who had received treatment for greater than 1 year and less than or equal to 5 years showed no such comparable increase in cytotoxic activity. Within the group of patients with untreated thyrotoxicosis it was found that K-cell cytotoxic activity was related to both goitre size and serum antibody titre. Thus patients with little or no goitre showed a highly significant elevation of cytotoxic activity whereas patients with moderate to large goitres gave values within the normal range. Similarly patients with no detectable serum thyroid autoantibodies showed high K-cell activity while patients with positive antibody titres did not. It was also shown that neither the absolute number nor the proportion of circulating T and B lymphocytes in patients with autoimmune thyroid disease as assessed by the sheep red cell rosette method and by indirect immunofluorescence was significantly different from that observed in the normal control population. No correlation was found between peripheral blood K-cell cytotoxic activity and the percentage of circulating null cells, i.e. 100-(percentage T + percentage B) in either patients or control subjects."}
{"id": "PMID:1086750", "title": "Acute phase proteins and C9 in patients with Behcet's syndrome and aphthous ulcers.", "content": "Estimation of the concentration of C9, C-reactive protein (CRP) and alpha1-antitrypsin in forty sera from patients with Behcet's syndrome and recurrent oral ulcers showed significantly increased amounts of C9 and CRP in Behcet's syndrome. The concentration of C9 was also significantly raised in recurrent oral ulceration, though to a lesser extent than in Behcet's syndrome. The assay C9 and CRP might be useful in the differential diagnosis of Behcet's syndrome, especially from recurrent oral ulcers. It is suggested that during epithelial inflammation in recurrent oral ulcers some of the acute phase proteins are increased and in some patients these may modulate the immunological mechanism in such a way as to induce a transition from focal oral ulceration to the multifocal Behcet's syndrome.", "contents": "Acute phase proteins and C9 in patients with Behcet's syndrome and aphthous ulcers. Estimation of the concentration of C9, C-reactive protein (CRP) and alpha1-antitrypsin in forty sera from patients with Behcet's syndrome and recurrent oral ulcers showed significantly increased amounts of C9 and CRP in Behcet's syndrome. The concentration of C9 was also significantly raised in recurrent oral ulceration, though to a lesser extent than in Behcet's syndrome. The assay C9 and CRP might be useful in the differential diagnosis of Behcet's syndrome, especially from recurrent oral ulcers. It is suggested that during epithelial inflammation in recurrent oral ulcers some of the acute phase proteins are increased and in some patients these may modulate the immunological mechanism in such a way as to induce a transition from focal oral ulceration to the multifocal Behcet's syndrome."}
{"id": "PMID:1086751", "title": "Receptors for Helix pomatia A haemagglutinin on leukaemic lymphocytes from patients with chronic lymphocytic leukaemia (CLL).", "content": "Blood lymphocytes from thirteen patients with CLL were studied for surface-bound Ig (SIg), Fc receptors (EA rosettes), receptors for sheep erythrocytes (E rosettes) and receptors for Helix pomatia A haemagglutinin (HP), a carbohydrate-binding protein with specificity for N-acetyl-D-galactosamine and related sugars. Fluorescein-labelled HP binds to subpopulations of human peripheral blood lymphocytes (PBL) treated with neuraminidase. In normal peripheral blood, HP binds to the T-lymphocytes while the majority of the B cells bearing surface-bound immunoglobulin do not have receptors for HP. In untreated CLL, HP binds to 90-100 percent of the neuraminidase-treated PBL. Almost all of the SI-G-POSITIVE CELLS IN CLL patients also have receptors for HP. Two groups of patients were found: in one the total fraction of SIg+ cells was less than or equal to 50 percent and about 30 percent of these lost their Ig during incubation at 37 degrees C. No such loss of SIg was revealed in the remaining patients where total SIg+ fraction was approximately 70 percent. These patients usually had higher blood lymphocyte counts, probably reflecting a more advanced disease. CLL patients in remission with low numbers of leukaemic cells also had low numbers of blood lymphocytes carrying both SIg and HP-receptors. It is concluded that leukaemic cells carry both HP receptors and SIg. Testing of this combination therefore provides a valuable new tool for monitoring patients with CLL.", "contents": "Receptors for Helix pomatia A haemagglutinin on leukaemic lymphocytes from patients with chronic lymphocytic leukaemia (CLL). Blood lymphocytes from thirteen patients with CLL were studied for surface-bound Ig (SIg), Fc receptors (EA rosettes), receptors for sheep erythrocytes (E rosettes) and receptors for Helix pomatia A haemagglutinin (HP), a carbohydrate-binding protein with specificity for N-acetyl-D-galactosamine and related sugars. Fluorescein-labelled HP binds to subpopulations of human peripheral blood lymphocytes (PBL) treated with neuraminidase. In normal peripheral blood, HP binds to the T-lymphocytes while the majority of the B cells bearing surface-bound immunoglobulin do not have receptors for HP. In untreated CLL, HP binds to 90-100 percent of the neuraminidase-treated PBL. Almost all of the SI-G-POSITIVE CELLS IN CLL patients also have receptors for HP. Two groups of patients were found: in one the total fraction of SIg+ cells was less than or equal to 50 percent and about 30 percent of these lost their Ig during incubation at 37 degrees C. No such loss of SIg was revealed in the remaining patients where total SIg+ fraction was approximately 70 percent. These patients usually had higher blood lymphocyte counts, probably reflecting a more advanced disease. CLL patients in remission with low numbers of leukaemic cells also had low numbers of blood lymphocytes carrying both SIg and HP-receptors. It is concluded that leukaemic cells carry both HP receptors and SIg. Testing of this combination therefore provides a valuable new tool for monitoring patients with CLL."}
{"id": "PMID:1086752", "title": "Peripheral blood lymphocyte populations in chronic liver disease.", "content": "Mature T lymphocyte concentrations are reduced, null cell concentrations are increased, and Fc receptor bearing (B and K) lymphocyte concentrations are normal, in the peripheral blood of patients with chronic hepatocellular or cholestatic liver disease. Some null cells can be stimulated by either thymosin or levamisole to form rosettes with sheep erythrocytes. These changes are present in viral, alcohol associated and 'autoimmune' liver disease and are therefore probably secondary phenomena relating to liver damage.", "contents": "Peripheral blood lymphocyte populations in chronic liver disease. Mature T lymphocyte concentrations are reduced, null cell concentrations are increased, and Fc receptor bearing (B and K) lymphocyte concentrations are normal, in the peripheral blood of patients with chronic hepatocellular or cholestatic liver disease. Some null cells can be stimulated by either thymosin or levamisole to form rosettes with sheep erythrocytes. These changes are present in viral, alcohol associated and 'autoimmune' liver disease and are therefore probably secondary phenomena relating to liver damage."}
{"id": "PMID:1086753", "title": "Study of lymphocyte subpopulations in normal humans and patients with systemic lupus erythematosus by fractionation of peripheral blood lymphocytes on a discontinuous Ficoll gradient.", "content": "Peripheral blood lymphocytes of thirty normal volunteers and fifty-two patients with systemic lupus erythematosus were fractionated using a discontinuous (5-30 percent) Ficoll gradient. Such fractionation permitted the isolation, identification of study of null cells, T cells and B cells. Patients with inactive SLE were found to have a cell distribution and responsiveness to PHA, Con A and Pokeweed mitogen (PWM) similar to controls. In contrast, patients with active SLE showed a significant decrease in T-cell fractions as well as a relative increase in null cells, a normal distribution of B cells, a marked reduction in responsiveness to Con A, a lesser reduction to PHA and only a minor reduction to PWM. With increasing disease activity, the number of null cells increased despite lymphopenia. Spontaneous lymphocyte transformation was observed in patients with SLE. This occurred predominantly in the fractions enriched in B cells and was observed both early (0-16 hr) and late (68-72 hr) in the lymphocyte cultures. The method of discontinuous Ficoll gradients is both versatile and reproducible with good correlations between isolated lymphoid subpopulations and disease activity.", "contents": "Study of lymphocyte subpopulations in normal humans and patients with systemic lupus erythematosus by fractionation of peripheral blood lymphocytes on a discontinuous Ficoll gradient. Peripheral blood lymphocytes of thirty normal volunteers and fifty-two patients with systemic lupus erythematosus were fractionated using a discontinuous (5-30 percent) Ficoll gradient. Such fractionation permitted the isolation, identification of study of null cells, T cells and B cells. Patients with inactive SLE were found to have a cell distribution and responsiveness to PHA, Con A and Pokeweed mitogen (PWM) similar to controls. In contrast, patients with active SLE showed a significant decrease in T-cell fractions as well as a relative increase in null cells, a normal distribution of B cells, a marked reduction in responsiveness to Con A, a lesser reduction to PHA and only a minor reduction to PWM. With increasing disease activity, the number of null cells increased despite lymphopenia. Spontaneous lymphocyte transformation was observed in patients with SLE. This occurred predominantly in the fractions enriched in B cells and was observed both early (0-16 hr) and late (68-72 hr) in the lymphocyte cultures. The method of discontinuous Ficoll gradients is both versatile and reproducible with good correlations between isolated lymphoid subpopulations and disease activity."}
{"id": "PMID:1086754", "title": "Cell suppression in PPD-induced blast specific response of human peripheral blood lymphocytes.", "content": "Secific stimulation of T-cells by PPD was inhibited by their autologous B cells. This inhibition was obtained with B cells separated either by depletion of E-RFC or by elution, with human IgG of lymphocytes bound to Sephadex beads coated with rabbit antibodies anti-human Fab fragments. The suppression was proportional to the number of B cells added to 10(6) T cells incubated with PPD and as previously reported was more marked in the case of B or T cells from BCG-vaccinated subjects with negative skin tests. The suppressive phenomenon required viable B cells and was inhibited by cycloheximide but was not altered by pretreatment of suppressor cells with actinomycin D or colchicine. It seems that B-suppressor cells interfere with recognition of PPD by T cells rather than with the proliferative phase of the specific blast response. Using various surface markers (i.e. Ig, C3 and Fc receptors) it was shown that the suppressor cells represent a subset of Ig-bearing B cells which do not carry Fc receptors.", "contents": "Cell suppression in PPD-induced blast specific response of human peripheral blood lymphocytes. Secific stimulation of T-cells by PPD was inhibited by their autologous B cells. This inhibition was obtained with B cells separated either by depletion of E-RFC or by elution, with human IgG of lymphocytes bound to Sephadex beads coated with rabbit antibodies anti-human Fab fragments. The suppression was proportional to the number of B cells added to 10(6) T cells incubated with PPD and as previously reported was more marked in the case of B or T cells from BCG-vaccinated subjects with negative skin tests. The suppressive phenomenon required viable B cells and was inhibited by cycloheximide but was not altered by pretreatment of suppressor cells with actinomycin D or colchicine. It seems that B-suppressor cells interfere with recognition of PPD by T cells rather than with the proliferative phase of the specific blast response. Using various surface markers (i.e. Ig, C3 and Fc receptors) it was shown that the suppressor cells represent a subset of Ig-bearing B cells which do not carry Fc receptors."}
{"id": "PMID:1086755", "title": "Influence of dietary protein restriction on immune competence. II. Effect on lymphoid tissue.", "content": "Weanling mice fed a 4 percent diet showed a generalized loss of lymphoid tissue which was greater than the loss of total body weight. This effect was greatest in the thymus greater than spleen greater than mesenteric lymph node. Cell loss was most pronounced during the 1st week on diets, then remained at stable levels for 3 weeks and showed a gradual rise thereafter. The effect was shown to be mediated partly by a cessation of growth in lymphoid organs due to the low protein intake and, secondly, an adrenal corticosteroid induced lympholysis which actually reduced cell numbers. Recirculating T cells and resident B cells were amongst the least affected cells whereas stem cells, non-migratory T cells and other reticuloendothelial cells were most depressed in numbers. At no stage was the germinal centre forming capacity of the mesenteric node lost although cell recruitment to antigenically stimulated nodes was diminished. During nutritional repletion the spleen, thymus and mesenteric node all showed different and characteristic regrowth. The spleen was most active initially and rapidly reconstituted haemopoietic cells and B cells. This was followed by the thymus which showed a delayed reinitiation of its normal growth kinetics which had been interrupted by the diet. The evidence suggested that full rehabilitation of the immune apparatus took place even after 2 months of nutritional deprivation.", "contents": "Influence of dietary protein restriction on immune competence. II. Effect on lymphoid tissue. Weanling mice fed a 4 percent diet showed a generalized loss of lymphoid tissue which was greater than the loss of total body weight. This effect was greatest in the thymus greater than spleen greater than mesenteric lymph node. Cell loss was most pronounced during the 1st week on diets, then remained at stable levels for 3 weeks and showed a gradual rise thereafter. The effect was shown to be mediated partly by a cessation of growth in lymphoid organs due to the low protein intake and, secondly, an adrenal corticosteroid induced lympholysis which actually reduced cell numbers. Recirculating T cells and resident B cells were amongst the least affected cells whereas stem cells, non-migratory T cells and other reticuloendothelial cells were most depressed in numbers. At no stage was the germinal centre forming capacity of the mesenteric node lost although cell recruitment to antigenically stimulated nodes was diminished. During nutritional repletion the spleen, thymus and mesenteric node all showed different and characteristic regrowth. The spleen was most active initially and rapidly reconstituted haemopoietic cells and B cells. This was followed by the thymus which showed a delayed reinitiation of its normal growth kinetics which had been interrupted by the diet. The evidence suggested that full rehabilitation of the immune apparatus took place even after 2 months of nutritional deprivation."}
{"id": "PMID:1086758", "title": "Ampicillin and an ester in experimental Hemophilus influenzae meningitis.", "content": "A new methoxymethyl ester of hetacillin is highly lipid-soluble, a property which was expected to enhance its penetration into the central nervous system (CNS). We compared the penetration of the ester and ampicillin into the cerebrospinal fluid (CSF) of normal rabbits and those with experimental Hemophilus influenzae meningitis. In normal rabbits treated by constant intravenous infusion, mean per cent penetration (see article) of the ester was four times as great as that of ampicillin (6.6 +/- 3.7% against 1.6 +/- 1.9%), and the difference in CSF drug levels attained was accentuated when bolus IV infusion was used. Drug concentration in brain tissue was three times as great in a rabbit treated with the ester (0.79 mug/gm against 0.26 mug/gm). In rabbits with meningitis, the ester again achieved higher CSF concentrations. Per cent penetration into CSF in infected rabbits was 23% for the ester and 13% for ampicillin. Bactericidal activity of the drugs was comparable; over 8 hr of treatment both drugs significantly reduced the CSF bacterial titers of infected animals. Our data demonstrated that the ester enters the CSF and CNS in higher concentration than ampicillin, with no loss in bactericidal activity.", "contents": "Ampicillin and an ester in experimental Hemophilus influenzae meningitis. A new methoxymethyl ester of hetacillin is highly lipid-soluble, a property which was expected to enhance its penetration into the central nervous system (CNS). We compared the penetration of the ester and ampicillin into the cerebrospinal fluid (CSF) of normal rabbits and those with experimental Hemophilus influenzae meningitis. In normal rabbits treated by constant intravenous infusion, mean per cent penetration (see article) of the ester was four times as great as that of ampicillin (6.6 +/- 3.7% against 1.6 +/- 1.9%), and the difference in CSF drug levels attained was accentuated when bolus IV infusion was used. Drug concentration in brain tissue was three times as great in a rabbit treated with the ester (0.79 mug/gm against 0.26 mug/gm). In rabbits with meningitis, the ester again achieved higher CSF concentrations. Per cent penetration into CSF in infected rabbits was 23% for the ester and 13% for ampicillin. Bactericidal activity of the drugs was comparable; over 8 hr of treatment both drugs significantly reduced the CSF bacterial titers of infected animals. Our data demonstrated that the ester enters the CSF and CNS in higher concentration than ampicillin, with no loss in bactericidal activity."}
{"id": "PMID:1086760", "title": "Immunobiology of the gastrointestinal tract.", "content": "The interplay between the gut and immune abnormality appears to be a logical extension of the thesis that secretory  IgA  is the major immunologic line of defense between the outside environment and the host. Thus immunologic deficiency, particularly of IgA and combined T- and B-lymphocyte abnormalites, profoundly influences gut integrity. Conversely, gut pathology is bound to interfere with immunologic function, so that both humoral and cellular immunity may be impaired. Finally, hypersensitivity phenomena in the gut, resulting in immune injury, may cause gastrointestinal disturbances. As better diagnostic tools have become available, more direct evidence of hypersensitivity immune injury has been described.", "contents": "Immunobiology of the gastrointestinal tract. The interplay between the gut and immune abnormality appears to be a logical extension of the thesis that secretory  IgA  is the major immunologic line of defense between the outside environment and the host. Thus immunologic deficiency, particularly of IgA and combined T- and B-lymphocyte abnormalites, profoundly influences gut integrity. Conversely, gut pathology is bound to interfere with immunologic function, so that both humoral and cellular immunity may be impaired. Finally, hypersensitivity phenomena in the gut, resulting in immune injury, may cause gastrointestinal disturbances. As better diagnostic tools have become available, more direct evidence of hypersensitivity immune injury has been described."}
{"id": "PMID:1086761", "title": "[Improved control of the pressure during use of the Sengstaken-Blakemore tube].", "content": "For the control of acute hemorrhage from esophageal varices by balloon tamponade a proper application of the Sengstaken-Blakemore tube is necessary to arrest bleeding and to minimize complications. A simple modification of the pressure measurement used up to now is described with other suitable applications. By this modified technique a correct pressure can be kept in the esophageal balloon spontaneously; its ease in handling in intensive care makes for better results in emergency management of balloon-tamponade in treatment of acute bleeding from esophageal varices.", "contents": "[Improved control of the pressure during use of the Sengstaken-Blakemore tube]. For the control of acute hemorrhage from esophageal varices by balloon tamponade a proper application of the Sengstaken-Blakemore tube is necessary to arrest bleeding and to minimize complications. A simple modification of the pressure measurement used up to now is described with other suitable applications. By this modified technique a correct pressure can be kept in the esophageal balloon spontaneously; its ease in handling in intensive care makes for better results in emergency management of balloon-tamponade in treatment of acute bleeding from esophageal varices."}
{"id": "PMID:1086763", "title": "Intestinal immunization with soluble bacterial antigens: the example of cholera toxoid.", "content": "The studies described are aimed at a better understanding of the intestinal immunological system and its role in protection against enteric infection. The cellular kinetics of the intestinal immune response to cholera toxoid were studied in rats and the protection afforded by toxoid immunization was studied in dogs. Memory was demonstrated in the gut immune system. Plasma cells containing IgA antitoxin appeared in large numbers in gut lamina propria when intraduodenal boosting followed either intraperitoneal priming or prolonged oral priming, intraperitoneal priming being the most efficient. Immunization by the intraperitoneal route alone produced no response in small bowel lamina propria. Lamina propria plasma cells were derived from precursors in Peyer's patches or mesenteric lymph nodes which migrated through the thoracic duct and systemic circulation before homing to the gut. Dogs were immunized parenterally with cholera toxin or toxoid and challenged orally with Vibrio cholerae. Protection correlated closely with serum antitoxin titres and was usually brief. Passive intravenous immunization with IgG antitoxin was also protective. In contrast, subcutaneous priming followed by oral boosting yielded longer protection without elevated serum antitoxin titres. Antitoxin was detected in jejunal washings only briefly after local boosting. The mechanism by which protection is prolonged is unclear but its greater duration after parenteral priming and oral boosting emphasizes the importance of stimulating the gut immune mechanism in attempts to immunize against enteric bacterial infections. The parenteral-oral squence may be an effective means of immunizing the intestine with non-replicating protein antigens.", "contents": "Intestinal immunization with soluble bacterial antigens: the example of cholera toxoid. The studies described are aimed at a better understanding of the intestinal immunological system and its role in protection against enteric infection. The cellular kinetics of the intestinal immune response to cholera toxoid were studied in rats and the protection afforded by toxoid immunization was studied in dogs. Memory was demonstrated in the gut immune system. Plasma cells containing IgA antitoxin appeared in large numbers in gut lamina propria when intraduodenal boosting followed either intraperitoneal priming or prolonged oral priming, intraperitoneal priming being the most efficient. Immunization by the intraperitoneal route alone produced no response in small bowel lamina propria. Lamina propria plasma cells were derived from precursors in Peyer's patches or mesenteric lymph nodes which migrated through the thoracic duct and systemic circulation before homing to the gut. Dogs were immunized parenterally with cholera toxin or toxoid and challenged orally with Vibrio cholerae. Protection correlated closely with serum antitoxin titres and was usually brief. Passive intravenous immunization with IgG antitoxin was also protective. In contrast, subcutaneous priming followed by oral boosting yielded longer protection without elevated serum antitoxin titres. Antitoxin was detected in jejunal washings only briefly after local boosting. The mechanism by which protection is prolonged is unclear but its greater duration after parenteral priming and oral boosting emphasizes the importance of stimulating the gut immune mechanism in attempts to immunize against enteric bacterial infections. The parenteral-oral squence may be an effective means of immunizing the intestine with non-replicating protein antigens."}
{"id": "PMID:1086769", "title": "Regulation of glucose-6-phosphate dehydrogenase activity in uterine tissue in organ culture.", "content": "An organ culture technique, employing a totally defined medium, was developed, in which the levels of glucose-6-P dehydrogenase (G6PD) activity in uterine tissue from estradiol-treated ovariectomized mature rats continued to increase in vitro for 18 h at a rate similar to that seen in vivo. Uterine G6PD levels did not increase in vitro in tissues from estrogen-deprived rats even if estradiol (10(-6)m) was added in vitro, but the administration of estradiol (5 mug/rat) in vivo for as little as 2 min permitted G6PD levels to increase by 0.03 units/uterus (from 0.075 to 0.10, units/uterus) after the 18 h incubation. The maximum increase of 0.08 units/uterus (from 0.147 to 0.230 units/uterus) was seen in uteri from rats which were given estradiol 12 h prior to sacrigice. Tissues from animals given estradiol for greater than 30 h exhibit a net decrease in G6PD levels under the in vitro conditions. The in vitro increase in G6PD activity is inhibited by the addition of either actinomycin D (5 mug/ml), cycloheximide (5 mug/ml), or cordycepin (150 mug/ml), or by the intrauterine injection of actinomycin D (10 mug/rat). Intrauterine administration of cycloheximide (100 mug/rat) inhibited the in vivo increase in enzyme activity; however, the enzyme levels increased after placement of uterine tissues from these animals into organ culture. The removal of cycloheximide, which was added at the beginning of incubation after the 12th h, restores the ability of the tissues to increase the G3PD activity, and this restoration is not blocked by the addition of actinomycin D, suggesting that the mRNA activity for uterine G6PD accumulated during in vitro inhibition of protein synthesis by cycloheximide. The in vitro increase in uterine G6PD is due to an increase in immunologically identifiable G6PD protein and this increase is due, at least in part, to an increase in the de novo synthesis of the enzyme, as measured by the incorporation of [14C]leucine into immunochemically isolated G6PD protein. These results suggest that once initiated in vivo by estradiol, the uterus is capable of continuing in vitro those events, including the synthesis of both RNA and protein, which result in an increased rate of synthesis of uterine G6PD.", "contents": "Regulation of glucose-6-phosphate dehydrogenase activity in uterine tissue in organ culture. An organ culture technique, employing a totally defined medium, was developed, in which the levels of glucose-6-P dehydrogenase (G6PD) activity in uterine tissue from estradiol-treated ovariectomized mature rats continued to increase in vitro for 18 h at a rate similar to that seen in vivo. Uterine G6PD levels did not increase in vitro in tissues from estrogen-deprived rats even if estradiol (10(-6)m) was added in vitro, but the administration of estradiol (5 mug/rat) in vivo for as little as 2 min permitted G6PD levels to increase by 0.03 units/uterus (from 0.075 to 0.10, units/uterus) after the 18 h incubation. The maximum increase of 0.08 units/uterus (from 0.147 to 0.230 units/uterus) was seen in uteri from rats which were given estradiol 12 h prior to sacrigice. Tissues from animals given estradiol for greater than 30 h exhibit a net decrease in G6PD levels under the in vitro conditions. The in vitro increase in G6PD activity is inhibited by the addition of either actinomycin D (5 mug/ml), cycloheximide (5 mug/ml), or cordycepin (150 mug/ml), or by the intrauterine injection of actinomycin D (10 mug/rat). Intrauterine administration of cycloheximide (100 mug/rat) inhibited the in vivo increase in enzyme activity; however, the enzyme levels increased after placement of uterine tissues from these animals into organ culture. The removal of cycloheximide, which was added at the beginning of incubation after the 12th h, restores the ability of the tissues to increase the G3PD activity, and this restoration is not blocked by the addition of actinomycin D, suggesting that the mRNA activity for uterine G6PD accumulated during in vitro inhibition of protein synthesis by cycloheximide. The in vitro increase in uterine G6PD is due to an increase in immunologically identifiable G6PD protein and this increase is due, at least in part, to an increase in the de novo synthesis of the enzyme, as measured by the incorporation of [14C]leucine into immunochemically isolated G6PD protein. These results suggest that once initiated in vivo by estradiol, the uterus is capable of continuing in vitro those events, including the synthesis of both RNA and protein, which result in an increased rate of synthesis of uterine G6PD."}
{"id": "PMID:1086764", "title": "Spontaneous rupture of colic-artery aneurysms: report of two cases.", "content": "Two cases of spontaneous rupture of colicartery aneurysms are presented, one involving the left colic artery and the other the right colic artery, and the literature on abdominal apoplexy and visceral arterial aneurysms is reviewed. In view of the potential for life-threatening hemorrhage, it is urged that, whenever feasible, visceral arterial aneurysms be resected prophylactically when discovered by angiography or at celiotomy.", "contents": "Spontaneous rupture of colic-artery aneurysms: report of two cases. Two cases of spontaneous rupture of colicartery aneurysms are presented, one involving the left colic artery and the other the right colic artery, and the literature on abdominal apoplexy and visceral arterial aneurysms is reviewed. In view of the potential for life-threatening hemorrhage, it is urged that, whenever feasible, visceral arterial aneurysms be resected prophylactically when discovered by angiography or at celiotomy."}
{"id": "PMID:1086770", "title": "Long-term survival following aortocoronary artery bypass. Analysis of 4,522 consecutive patients.", "content": "At the Texas Heart Institute, from October 1969 through December 1974 a consecutive series of 4,522 patients who underwent aortocoronary bypass procedures was analyzed. Our experience with this procedure over a 5-year period demonstrated a decreasing operative and long-term mortality. This included patients who had aortocoronary bypass alone or in combination with other procedures such as resection of a left ventricular aneurysm or aortic and mitral valve procedures. Actuarial follow-up during the 5 years revealed mortality to be approximately 2.5% per year. Current operative mortality is less than 3.5% regardless of the number of grafts implanted. The rate of late myocardial infarction was only 4% for the entire series. Coronary artery bypass can be safely performed with minimal mortality and increased long-term survival.", "contents": "Long-term survival following aortocoronary artery bypass. Analysis of 4,522 consecutive patients. At the Texas Heart Institute, from October 1969 through December 1974 a consecutive series of 4,522 patients who underwent aortocoronary bypass procedures was analyzed. Our experience with this procedure over a 5-year period demonstrated a decreasing operative and long-term mortality. This included patients who had aortocoronary bypass alone or in combination with other procedures such as resection of a left ventricular aneurysm or aortic and mitral valve procedures. Actuarial follow-up during the 5 years revealed mortality to be approximately 2.5% per year. Current operative mortality is less than 3.5% regardless of the number of grafts implanted. The rate of late myocardial infarction was only 4% for the entire series. Coronary artery bypass can be safely performed with minimal mortality and increased long-term survival."}
{"id": "PMID:1086771", "title": "The intestinal mucosal lesions in shock. I. Studies on the pathogenesis.", "content": "Mucosal lesions were produced in feline small intestine by evoking a simulated intestinal shock (local hypotension at 30 mm Hg and stimulation of regional sympathetic vasoconstrictor nerves at 6 Hz for 2 h). The degree of mucosal damage was correlated to the level of intestinal blood flow. Microscopically characteristic lesions developed regularly in the small intestinal mucosa when intestinal blood flow was reduced below 12 ml/min X 100 g during the regional shock. The mucosal damage was graded histologically. No difference was found between untreated controls and cats in which the intestinal lumen was perfused with nitrogenated saline. Perfusion with oxygenated saline and i.v. injections of methylprednisolone on the other hand, prevented almost completely the development of the lesions. Albumin, activated charcoal and aprotinin instilled into the intestinal lumen reduced to some extent the mucosal damage. The obtained data support the view that hypoxia is the key factor in the pathogenesis of the mucosal lesions. However, epithelial and intraluminal enzymes are probably important contributing factors.", "contents": "The intestinal mucosal lesions in shock. I. Studies on the pathogenesis. Mucosal lesions were produced in feline small intestine by evoking a simulated intestinal shock (local hypotension at 30 mm Hg and stimulation of regional sympathetic vasoconstrictor nerves at 6 Hz for 2 h). The degree of mucosal damage was correlated to the level of intestinal blood flow. Microscopically characteristic lesions developed regularly in the small intestinal mucosa when intestinal blood flow was reduced below 12 ml/min X 100 g during the regional shock. The mucosal damage was graded histologically. No difference was found between untreated controls and cats in which the intestinal lumen was perfused with nitrogenated saline. Perfusion with oxygenated saline and i.v. injections of methylprednisolone on the other hand, prevented almost completely the development of the lesions. Albumin, activated charcoal and aprotinin instilled into the intestinal lumen reduced to some extent the mucosal damage. The obtained data support the view that hypoxia is the key factor in the pathogenesis of the mucosal lesions. However, epithelial and intraluminal enzymes are probably important contributing factors."}
{"id": "PMID:1086772", "title": "The intestinal mucosal lesions in shock. II. The relationship between the mucosal lesions and the cardiovascular derangement following regional shock.", "content": "The relationship between the mucosal lesions in the gut, observed after a 2-hour period of regional hypotension, and the blood pressure fall seen after the hypotensive period was investigated in cats. Untreated controls were compared to animals treated with intraluminal perfusion with nitrogenated or oxygenated saline or treated with intraluminal instillation of albumin, activated charcoal or aprotinin or i.v. injections of methylprednisolone. Untreated controls and cats perfused with nitrogenated saline exhibited a pronounced reduction in arterial blood pressure during the first posthypotensive hour. In the animals treated with methylprednisolone or perfused intraluminally with oxygenated saline only a small fall of blood pressure was observed. In the remaining groups of animals a moderate blood pressure reduction was noted. These results suggest a causal relationship between the intestinal mucosal damage and the posthypotensive cardiovascular derangement possibly via the release of cardiotoxic material from the hypoxic intestinal villi.", "contents": "The intestinal mucosal lesions in shock. II. The relationship between the mucosal lesions and the cardiovascular derangement following regional shock. The relationship between the mucosal lesions in the gut, observed after a 2-hour period of regional hypotension, and the blood pressure fall seen after the hypotensive period was investigated in cats. Untreated controls were compared to animals treated with intraluminal perfusion with nitrogenated or oxygenated saline or treated with intraluminal instillation of albumin, activated charcoal or aprotinin or i.v. injections of methylprednisolone. Untreated controls and cats perfused with nitrogenated saline exhibited a pronounced reduction in arterial blood pressure during the first posthypotensive hour. In the animals treated with methylprednisolone or perfused intraluminally with oxygenated saline only a small fall of blood pressure was observed. In the remaining groups of animals a moderate blood pressure reduction was noted. These results suggest a causal relationship between the intestinal mucosal damage and the posthypotensive cardiovascular derangement possibly via the release of cardiotoxic material from the hypoxic intestinal villi."}
{"id": "PMID:1086774", "title": "Mitogen-induced membrane changes and cell proliferation in T lymphocyte subpopulations.", "content": "Rabbit thymus-dependent lymphocytes were exposed to phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM) or anti-immunoglobulin at various stages of maturation. Proliferation (induction of DNA synthesis) and early membrane events (turnover of membrane phospholipids) were measured in neonatal thymocytes, normal adult thymocytes, prednisolone-resistant thymocytes and lymph node lymphocytes. In immature thymocytes PHA induced only a marginal increase in DNA synthesis. The mitotic response increased with maturation, but only peripheral T lymphocytes exhibited maximum stimulation. Con A and PWM were able to induce DNA synthesis in immature thymocytes and the degree of stimulation was shown to increase with maturation. In contrast to the different degree of proliferation of thymocytes induced by PHA or Con A the incorporation of [14C]oleate, [14C]choline or [14C]acetate into phospholipids was stimulated to the same degree by these lectins. Reactivity of T lymphocytes, as measured by early membrane changes at different stages of maturation, to different T cell mitogens appears to be identical. Differences in degree of cell proliferation therefore may be secondary phenomena due, in part, to tissue culture conditions. Reactivity to mitogens as measured by phospholipid turnover appears to be an early acquired function in the maturation of lymphocytes of the T cell line.", "contents": "Mitogen-induced membrane changes and cell proliferation in T lymphocyte subpopulations. Rabbit thymus-dependent lymphocytes were exposed to phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM) or anti-immunoglobulin at various stages of maturation. Proliferation (induction of DNA synthesis) and early membrane events (turnover of membrane phospholipids) were measured in neonatal thymocytes, normal adult thymocytes, prednisolone-resistant thymocytes and lymph node lymphocytes. In immature thymocytes PHA induced only a marginal increase in DNA synthesis. The mitotic response increased with maturation, but only peripheral T lymphocytes exhibited maximum stimulation. Con A and PWM were able to induce DNA synthesis in immature thymocytes and the degree of stimulation was shown to increase with maturation. In contrast to the different degree of proliferation of thymocytes induced by PHA or Con A the incorporation of [14C]oleate, [14C]choline or [14C]acetate into phospholipids was stimulated to the same degree by these lectins. Reactivity of T lymphocytes, as measured by early membrane changes at different stages of maturation, to different T cell mitogens appears to be identical. Differences in degree of cell proliferation therefore may be secondary phenomena due, in part, to tissue culture conditions. Reactivity to mitogens as measured by phospholipid turnover appears to be an early acquired function in the maturation of lymphocytes of the T cell line."}
{"id": "PMID:1086775", "title": "B cell tolerance induced by polymeric antigens. IV. Antigen-mediated inhibition of antibody-forming cells.", "content": "The role of antibody-forming cell (AFC) blockade in the genesis of hapten-specific tolerance induced by hapten (2,4-dinitrophenyl)-coupled pneumococcal polysaccharide (DNP-lys-S3) was investigated. Tolerizing doses of DNP-lys-S3 given to mice making antibodies to DNP-hemocyanin a day before assay markedly reduced numbers of detectable anti-DNP AFC. Furthermore, anti-DNP AFC were specifically inhibited by brief in vitro incubation with DNP-lys-S3, followed by extensive washing. This inhibition requires multivalent binding of tolerogen to immunoglobulin receptors on AFC, and appears to result from a decrease in the rate of antibody secretion per cell. A variety of data indicate that IgM AFC are more susceptible to blockade than IgG AFC, and the susceptibility of both cell types decreases with time after immunization. This probably reflects differences in the density and in the rate of loss of surface immunoglobulin receptors on IgM and IgG AFC and their immediate precursors. However, we conclude that under \"conventional\" tolerizing conditions, i.e. when tolerogen is given prior to immunogen, DNP-lys-S3 suppresses antibody formation primarily by inactivating DNP-specific precursor cells. AFC blockade is therefore probably only relevant in the suppression of (primary?) IgM responses by large doses of tolerogen. The relative importance of precursor inactivation and AFC blockade in tolerogenesis of IgM responses to T cell-independent antigens (such as S3) remains to be elucidated.", "contents": "B cell tolerance induced by polymeric antigens. IV. Antigen-mediated inhibition of antibody-forming cells. The role of antibody-forming cell (AFC) blockade in the genesis of hapten-specific tolerance induced by hapten (2,4-dinitrophenyl)-coupled pneumococcal polysaccharide (DNP-lys-S3) was investigated. Tolerizing doses of DNP-lys-S3 given to mice making antibodies to DNP-hemocyanin a day before assay markedly reduced numbers of detectable anti-DNP AFC. Furthermore, anti-DNP AFC were specifically inhibited by brief in vitro incubation with DNP-lys-S3, followed by extensive washing. This inhibition requires multivalent binding of tolerogen to immunoglobulin receptors on AFC, and appears to result from a decrease in the rate of antibody secretion per cell. A variety of data indicate that IgM AFC are more susceptible to blockade than IgG AFC, and the susceptibility of both cell types decreases with time after immunization. This probably reflects differences in the density and in the rate of loss of surface immunoglobulin receptors on IgM and IgG AFC and their immediate precursors. However, we conclude that under \"conventional\" tolerizing conditions, i.e. when tolerogen is given prior to immunogen, DNP-lys-S3 suppresses antibody formation primarily by inactivating DNP-specific precursor cells. AFC blockade is therefore probably only relevant in the suppression of (primary?) IgM responses by large doses of tolerogen. The relative importance of precursor inactivation and AFC blockade in tolerogenesis of IgM responses to T cell-independent antigens (such as S3) remains to be elucidated."}
{"id": "PMID:1086776", "title": "Lymphocyte stimulation by protein A of Staphylococcus aureus.", "content": "Protein A from Staphylococcus aureus (SpA) is known to bind to the Fc region of most mammalian IgG classes. In the present article data are presented showing that SpA is a highly efficient mitogen for human peripheral B lymphocytes, with no detectable activity for T lymphocytes. In order to achieve optimal stimulating conditions SpA should be presented to the lymphocytes on an insoluble matrix, such as the SpA-positive bacteria themselves or SpA covalently attached to Sephadex or Sepharose beads. Using such conditions SpA is equivalent with regard to stimulatory capacity for B lymphocytes as phytohemagglutinin is for the human T lymphocytes. Specificity controls proved beyond doubt that SpA and not any other contaminating product is the B cell mitogen. It is concluded that SpA as an inducer of human B lymphocyte division might serve as a highly useful assay in the clinical assessment of B lymphocyte function. It should also be a suitable tool in the fine analysis of B lymphocyte activation via the specific interactions with surface IgG molecules.", "contents": "Lymphocyte stimulation by protein A of Staphylococcus aureus. Protein A from Staphylococcus aureus (SpA) is known to bind to the Fc region of most mammalian IgG classes. In the present article data are presented showing that SpA is a highly efficient mitogen for human peripheral B lymphocytes, with no detectable activity for T lymphocytes. In order to achieve optimal stimulating conditions SpA should be presented to the lymphocytes on an insoluble matrix, such as the SpA-positive bacteria themselves or SpA covalently attached to Sephadex or Sepharose beads. Using such conditions SpA is equivalent with regard to stimulatory capacity for B lymphocytes as phytohemagglutinin is for the human T lymphocytes. Specificity controls proved beyond doubt that SpA and not any other contaminating product is the B cell mitogen. It is concluded that SpA as an inducer of human B lymphocyte division might serve as a highly useful assay in the clinical assessment of B lymphocyte function. It should also be a suitable tool in the fine analysis of B lymphocyte activation via the specific interactions with surface IgG molecules."}
{"id": "PMID:1086777", "title": "Studies on the activation of mouse bone marrow-derived macrophages by the macrophage cytotoxicity factor (MCF).", "content": "The capacity of bone marrow-derived macrophages to be rendered cytotoxic by macrophage cytotoxicity factor (MCF) at different stages of maturation was tested. Under in vitro conditions not only mature macrophages but also nonadherent precursor cells were successfully activated. Mature adherent bone marrow macrophages can be rendered cytotoxic for up to three weeks in tissue culture. Cytotoxic effects are demonstrable even at a killer target ratio of 0.5:1. Granulocytes do not respond to activation with MCF. Bone marrow-derived macrophages can be collected at early stages of maturation, when they grow in suspension or have developed only loose adherence. Macrophages which have differentiated in vitro from bone marrow never show any signs of nonspecific activation as measured by our cytotoxic assay system.", "contents": "Studies on the activation of mouse bone marrow-derived macrophages by the macrophage cytotoxicity factor (MCF). The capacity of bone marrow-derived macrophages to be rendered cytotoxic by macrophage cytotoxicity factor (MCF) at different stages of maturation was tested. Under in vitro conditions not only mature macrophages but also nonadherent precursor cells were successfully activated. Mature adherent bone marrow macrophages can be rendered cytotoxic for up to three weeks in tissue culture. Cytotoxic effects are demonstrable even at a killer target ratio of 0.5:1. Granulocytes do not respond to activation with MCF. Bone marrow-derived macrophages can be collected at early stages of maturation, when they grow in suspension or have developed only loose adherence. Macrophages which have differentiated in vitro from bone marrow never show any signs of nonspecific activation as measured by our cytotoxic assay system."}
{"id": "PMID:1086778", "title": "Identification of a high molecular weight protein on the surface of murine thymus and thymus-dependent cells.", "content": "Rabbit anti-mouse Ig reacted with mouse thymocytes resulting in the formation of caps which were shed into the medium and subsequently injected into rabbits. The antiserum from these animals (AMTP) reacted strongly with thymocytes and peripheral T cells and weakly with B cells. The antiserum did not react via the Thy-1 antigen and could be made specific for T lymphocytes by absorption with B lymphocytes. By surface labeling of lymphocytes with 125I, it could be shown that the major T lymphocyte antigen recognized by AMTP was one, or possibly two, large, single chain molecules with a molecular weight of approximately 200000. This molecule was not Ig and, furthermore, the AMTP did not react with cell surface Ig of B lymphocytes. The implications of this finding for previous reports on the existence of immunoglobulin on T lymphocytes are discussed.", "contents": "Identification of a high molecular weight protein on the surface of murine thymus and thymus-dependent cells. Rabbit anti-mouse Ig reacted with mouse thymocytes resulting in the formation of caps which were shed into the medium and subsequently injected into rabbits. The antiserum from these animals (AMTP) reacted strongly with thymocytes and peripheral T cells and weakly with B cells. The antiserum did not react via the Thy-1 antigen and could be made specific for T lymphocytes by absorption with B lymphocytes. By surface labeling of lymphocytes with 125I, it could be shown that the major T lymphocyte antigen recognized by AMTP was one, or possibly two, large, single chain molecules with a molecular weight of approximately 200000. This molecule was not Ig and, furthermore, the AMTP did not react with cell surface Ig of B lymphocytes. The implications of this finding for previous reports on the existence of immunoglobulin on T lymphocytes are discussed."}
{"id": "PMID:1086779", "title": "Suppressor cell induction in vitro. II. Cellular requirements of suppressor cell induction.", "content": "The cellular requirements for the induction of antigen-specific suppressor cells (SC) in vitro were studied. Macrophage depletion, by either nylon wool or polystyrene columns or iron (and magnet) treatment or a combination of methods, did not diminish SC induction, in contrast to its effect on helper cell induction. The requirement for T-T interaction in SC induction using splenic T cells was studied by the use of adult thymectomy and anti-lymphocyte serum, which selectively deplete short-lived and recirculating T cells respectively. Since suppressor cell induction was markedly diminished, or abolished by either procedure, but reconstituted by mixing these 2 populations, it was concluded that there was T-T interaction in the development of SC, just as there is in the induction of other T cell reactions, such as the graft-versus-host response, the T killer cell response, the helper cell response and, tentatively, delayed hypersensitivity. By the use of double-chamber tissue culture flasks, with the 2 cell populations separated by a cell impermeable membrane, it was found that T-T interaction does not require cell contact and is thus mediated by factor(s). The direct precursor of antigen-specific suppressor cells is resistant to adult thymectomy but sensitive to anti-lymphocyte serum.", "contents": "Suppressor cell induction in vitro. II. Cellular requirements of suppressor cell induction. The cellular requirements for the induction of antigen-specific suppressor cells (SC) in vitro were studied. Macrophage depletion, by either nylon wool or polystyrene columns or iron (and magnet) treatment or a combination of methods, did not diminish SC induction, in contrast to its effect on helper cell induction. The requirement for T-T interaction in SC induction using splenic T cells was studied by the use of adult thymectomy and anti-lymphocyte serum, which selectively deplete short-lived and recirculating T cells respectively. Since suppressor cell induction was markedly diminished, or abolished by either procedure, but reconstituted by mixing these 2 populations, it was concluded that there was T-T interaction in the development of SC, just as there is in the induction of other T cell reactions, such as the graft-versus-host response, the T killer cell response, the helper cell response and, tentatively, delayed hypersensitivity. By the use of double-chamber tissue culture flasks, with the 2 cell populations separated by a cell impermeable membrane, it was found that T-T interaction does not require cell contact and is thus mediated by factor(s). The direct precursor of antigen-specific suppressor cells is resistant to adult thymectomy but sensitive to anti-lymphocyte serum."}
{"id": "PMID:1086780", "title": "Effects of levamisole on spontaneous rosette-forming cells in murine spleen.", "content": "Levamisole, an anti-anergic chemotherapeutic agent, is shown to depress the azathioprine sensitivity of rosette-forming cells (RFC) in the spleen of normal mice, and to restore the azathioprine sensitivity of RFC in the spleen of adult thymectomized mice. Using the fully active dose of 1.25 mg/kg i.v., the restoration effect was present 15 h after treatment with the drug and almost disappeared after 48 h. Levamisole was ineffective on RFC in vitro. These results suggest that Levamisole might interfere with the reactivity of T lymphocytes as has been described for drugs interfering with cyclic nucleotide metabolism.", "contents": "Effects of levamisole on spontaneous rosette-forming cells in murine spleen. Levamisole, an anti-anergic chemotherapeutic agent, is shown to depress the azathioprine sensitivity of rosette-forming cells (RFC) in the spleen of normal mice, and to restore the azathioprine sensitivity of RFC in the spleen of adult thymectomized mice. Using the fully active dose of 1.25 mg/kg i.v., the restoration effect was present 15 h after treatment with the drug and almost disappeared after 48 h. Levamisole was ineffective on RFC in vitro. These results suggest that Levamisole might interfere with the reactivity of T lymphocytes as has been described for drugs interfering with cyclic nucleotide metabolism."}
{"id": "PMID:1086781", "title": "Lymphocyte-mediated cytotoxicity against tumor cells II. Characteristics and tissue distribution of concanavalin A-activated cytotoxic effector cells.", "content": "In vivo or in vitro activation of cytotoxic effector lymphocytes by concanavalin A (Con A) has been shown to result in a population of effector cells exhibiting a degree of immunological specificity when Con A was present during the cytotoxic assay (Waterfield, J.D. et al., Cell. Immunol. 1975. 17:392). In this communication we have characterized the Con A-activated effector cell by physical criteria and tried to assign it to a given subpopulation of thymus-derived lymphocytes. It was shown that macrophages played no role in target cell lysis in this system, nor was their presence required for Con A activation of the effector cell. The effector cell proved insensitive to anti-theta serum, but was classified as a T cell by the enrichment of cytotoxicity when a purified population of T cells was activated by Con A in vitro and by the failure to activate the effector cell in nude mice. Precursors of the effector cell were shown to reside primarily in the spleen, to be radioresistant up to 400 R, and to be short-lived after adult thymectomy. Thus, the effector cell can be classified with the T1 population of T cells and has similar characteristics as a cytolytically active cell described by Stobo et al. (J. Immunol. 1973. 110: 362, 652).", "contents": "Lymphocyte-mediated cytotoxicity against tumor cells II. Characteristics and tissue distribution of concanavalin A-activated cytotoxic effector cells. In vivo or in vitro activation of cytotoxic effector lymphocytes by concanavalin A (Con A) has been shown to result in a population of effector cells exhibiting a degree of immunological specificity when Con A was present during the cytotoxic assay (Waterfield, J.D. et al., Cell. Immunol. 1975. 17:392). In this communication we have characterized the Con A-activated effector cell by physical criteria and tried to assign it to a given subpopulation of thymus-derived lymphocytes. It was shown that macrophages played no role in target cell lysis in this system, nor was their presence required for Con A activation of the effector cell. The effector cell proved insensitive to anti-theta serum, but was classified as a T cell by the enrichment of cytotoxicity when a purified population of T cells was activated by Con A in vitro and by the failure to activate the effector cell in nude mice. Precursors of the effector cell were shown to reside primarily in the spleen, to be radioresistant up to 400 R, and to be short-lived after adult thymectomy. Thus, the effector cell can be classified with the T1 population of T cells and has similar characteristics as a cytolytically active cell described by Stobo et al. (J. Immunol. 1973. 110: 362, 652)."}
{"id": "PMID:1086782", "title": "Activation of T lymphocytes to M locus determinants in vivo. I. Quantitation of T cell proliferation and migration into thoracic duct lymph.", "content": "Proliferative responses to M locus (Mls) determinants were investigated in vivo by injecting T cell-containing populations intravenously into irradiated H2-identical, Mls-incompatible mice. Responses were studied 1-6 days later by measuring levels of radioactivity in the spleen and lymph nodes of the recipient 45 min after intravenous injection of tritiated thymidine. High responses were observed against Mlsa and Mlsd determinants, i.e. determinants which give strong proliferative responses in vitro. With injection of thoracic duct lymphocytes (TDL), lymph node or thymus cells, the responses, both in the spleen and the lymph nodes, reached a peak at day 3-4 and then fell sharply. This was in marked contrast to the stimulation observed with H-2-incompatible mice where, with TDL or lymph node cells (though not with thymus cells), responses were low at day 3-4 but reached high levels at day 6. Significant responses were also observed against Mlsb and Mlsc determinants, i.e. determinants which stimulate poorly in vitro. The kinetics of this response differed from that against Mlsa or Mlsd determinants in in that proliferation was very low at day 4 but high at day 6. No evidence was found that the responses observed reflected \"back-stimulation\" by the host. Since congenic resistant strains were not used, the possibility that the stimulation observed was in fact due to minor transplantation antigens rather than Mls determinants could not be excluded. Large numbers of blast cells appeared in thoracic duct lymph of mice sustaining responses to Mlsa or Mlsd determinants. Nearly all of these cells had recently synthesized DNA and entered the lymph after day 3.", "contents": "Activation of T lymphocytes to M locus determinants in vivo. I. Quantitation of T cell proliferation and migration into thoracic duct lymph. Proliferative responses to M locus (Mls) determinants were investigated in vivo by injecting T cell-containing populations intravenously into irradiated H2-identical, Mls-incompatible mice. Responses were studied 1-6 days later by measuring levels of radioactivity in the spleen and lymph nodes of the recipient 45 min after intravenous injection of tritiated thymidine. High responses were observed against Mlsa and Mlsd determinants, i.e. determinants which give strong proliferative responses in vitro. With injection of thoracic duct lymphocytes (TDL), lymph node or thymus cells, the responses, both in the spleen and the lymph nodes, reached a peak at day 3-4 and then fell sharply. This was in marked contrast to the stimulation observed with H-2-incompatible mice where, with TDL or lymph node cells (though not with thymus cells), responses were low at day 3-4 but reached high levels at day 6. Significant responses were also observed against Mlsb and Mlsc determinants, i.e. determinants which stimulate poorly in vitro. The kinetics of this response differed from that against Mlsa or Mlsd determinants in in that proliferation was very low at day 4 but high at day 6. No evidence was found that the responses observed reflected \"back-stimulation\" by the host. Since congenic resistant strains were not used, the possibility that the stimulation observed was in fact due to minor transplantation antigens rather than Mls determinants could not be excluded. Large numbers of blast cells appeared in thoracic duct lymph of mice sustaining responses to Mlsa or Mlsd determinants. Nearly all of these cells had recently synthesized DNA and entered the lymph after day 3."}
{"id": "PMID:1086783", "title": "Role of macrophages in the generation of T helper cells. IV. Nature of genetically related factor derived from macrophages incubated with soluble antigens.", "content": "Macrophages incubated with soluble antigens for 1-4 days release factors capable of inducing T helper cells. The nature and some of the properties of this genetically related macrophage factor (GRF) were investigated. By use of immunoadsorbents, GRF was shown to contain I region-coded products (Ia antigens) linked to a small antigenic fragment. The genes coding for the Ia antigens detectable in GRF lie in the I region of the H-2 complex. GRF does not react with anti-immunoglobulin, anti-C3 or anti-human beta2-microglobulin antisera, and its activity cannot be removed by antigen immunoadsorbents. GRF is heat-labile, sensitive to proteolytic enzymes and has a molecular weight of about 55 000 Daltons. The relationship of GRF to other factors known to contain I region-coded products and reported to be effective in cell cooperation is discussed.", "contents": "Role of macrophages in the generation of T helper cells. IV. Nature of genetically related factor derived from macrophages incubated with soluble antigens. Macrophages incubated with soluble antigens for 1-4 days release factors capable of inducing T helper cells. The nature and some of the properties of this genetically related macrophage factor (GRF) were investigated. By use of immunoadsorbents, GRF was shown to contain I region-coded products (Ia antigens) linked to a small antigenic fragment. The genes coding for the Ia antigens detectable in GRF lie in the I region of the H-2 complex. GRF does not react with anti-immunoglobulin, anti-C3 or anti-human beta2-microglobulin antisera, and its activity cannot be removed by antigen immunoadsorbents. GRF is heat-labile, sensitive to proteolytic enzymes and has a molecular weight of about 55 000 Daltons. The relationship of GRF to other factors known to contain I region-coded products and reported to be effective in cell cooperation is discussed."}
{"id": "PMID:1086784", "title": "B cell tolerance induced by polymeric antigens. V. Different avidities of primed and virgin precursor cells for paucivalent antigen.", "content": "We have compared the avidity of virgin and primed hapten-specific precursor cells for DNP-lys-S3 (dinitrophenylated type 3 pneumococcal polysaccharide) by measuring its capacity to prevent them from binding highly radioactive DNP-hemocyanin, and thereby protect them from committing hapten-specific \"suicide\". Moderately substituted conjugates (e.g. DNP-lys2.7S3) protected virgin and memory B cells impartially. In contrast, DNP-lys0.6S3 protected memory cells efficiently, but protected virgin cells only at considerably higher concentrations. These results are consistent with previous evidence - from this and other systems - that there is a higher density of receptors on primed than on unprimed B cells - the significance of which is discussed.", "contents": "B cell tolerance induced by polymeric antigens. V. Different avidities of primed and virgin precursor cells for paucivalent antigen. We have compared the avidity of virgin and primed hapten-specific precursor cells for DNP-lys-S3 (dinitrophenylated type 3 pneumococcal polysaccharide) by measuring its capacity to prevent them from binding highly radioactive DNP-hemocyanin, and thereby protect them from committing hapten-specific \"suicide\". Moderately substituted conjugates (e.g. DNP-lys2.7S3) protected virgin and memory B cells impartially. In contrast, DNP-lys0.6S3 protected memory cells efficiently, but protected virgin cells only at considerably higher concentrations. These results are consistent with previous evidence - from this and other systems - that there is a higher density of receptors on primed than on unprimed B cells - the significance of which is discussed."}
{"id": "PMID:1086785", "title": "Lymphocyte proliferation in vitro induced by soluble protein antigens. II. Cellular requirements.", "content": "Immune guinea pig lymph node cells were fractionated on Ig anti-Ig or HSA anti-HSA affinity columns or on plastic surface in medium containing carbonyl iron. These techniques selectively removed B lymphocytes, K lymphocytes or adherent cells. The residual cells (Fc receptor-negative T lymphocytes) responded to soluble antigen in vitro in the same way or even better compared with nonfractionated cells. In addition, there was no indication that antigen-antibody complexes were superior to antigen in triggering lymph node cells or purified lymph code T lymphocytes into DNA synthesis. The results obtained suggested that memory T lymphocytes can be stimulated by antigen autonomously.", "contents": "Lymphocyte proliferation in vitro induced by soluble protein antigens. II. Cellular requirements. Immune guinea pig lymph node cells were fractionated on Ig anti-Ig or HSA anti-HSA affinity columns or on plastic surface in medium containing carbonyl iron. These techniques selectively removed B lymphocytes, K lymphocytes or adherent cells. The residual cells (Fc receptor-negative T lymphocytes) responded to soluble antigen in vitro in the same way or even better compared with nonfractionated cells. In addition, there was no indication that antigen-antibody complexes were superior to antigen in triggering lymph node cells or purified lymph code T lymphocytes into DNA synthesis. The results obtained suggested that memory T lymphocytes can be stimulated by antigen autonomously."}
{"id": "PMID:1086786", "title": "The immunological properties of haptens coupled to thymus-independent carrier molecules. IV. The IgG response to dinitrophenylated Ficoll.", "content": "Dinitrophenylated polysucrose (DNP-Ficoll) elicits T cell-independent IgM anti-DNP antibody formation in mice. This antigen also elicits a heterogeneous IgG1 and IgG2 anti-DNP response, which is operationally as T-independent as the IgM response. However, a concomitant graft-versus-host reaction markedly enhances the IgG response (allogeneic effect). These results confirm those of others, indicating that a certain proportion of the precursors of IgG-producing cells can be triggered by some T-independent antigens. However, our results suggest that even with such antigens optimal triggering of IgG precursors requires T cell help.", "contents": "The immunological properties of haptens coupled to thymus-independent carrier molecules. IV. The IgG response to dinitrophenylated Ficoll. Dinitrophenylated polysucrose (DNP-Ficoll) elicits T cell-independent IgM anti-DNP antibody formation in mice. This antigen also elicits a heterogeneous IgG1 and IgG2 anti-DNP response, which is operationally as T-independent as the IgM response. However, a concomitant graft-versus-host reaction markedly enhances the IgG response (allogeneic effect). These results confirm those of others, indicating that a certain proportion of the precursors of IgG-producing cells can be triggered by some T-independent antigens. However, our results suggest that even with such antigens optimal triggering of IgG precursors requires T cell help."}
{"id": "PMID:1086787", "title": "Migratory patterns of B lymphocytes. V. Surface Ig and migration properties of density gradient-separated bursa cells.", "content": "A study was made of bovine serum albumin density gradient-separated bursa cells with respect to their distribution on the gradients, % cytoxicity with anti-Ig + complement (C) or anti-mu + C, and localization of fractions in irradiated syngeneic recipients. Layer A showed a higher cytotoxicity with anti-Ig + C or anti-mu + C than the others; layer D showed the lowest % cytotoxicity, although a large % of the cells not killed by anti-Ig were killed by anti-bursa serum + C. The C + D layers contained a much larger fraction of the Ig+ cells from the bursa in the 1--4-week-old than in the 6--12-week-old animals, suggesting a relative shift towards lower density of Ig+ bursa cells with age. When [3H]adenosine-labeled bursa cells from density gradient fractions were transferred, the % injected radioactivity found in recipient spleens was higher for fractions A and B than for fraction C + D. While 50--60 % of the cells from fractions A and B which localized in spleen were found in germinal centers and follicles, a much lower % of the cells from fraction C + D seen in spleen localized in these areas. The results were in agreement with those of previous studies suggesting that it is primarily the Ig-bearing population among bursa cells which localizes in follicles.", "contents": "Migratory patterns of B lymphocytes. V. Surface Ig and migration properties of density gradient-separated bursa cells. A study was made of bovine serum albumin density gradient-separated bursa cells with respect to their distribution on the gradients, % cytoxicity with anti-Ig + complement (C) or anti-mu + C, and localization of fractions in irradiated syngeneic recipients. Layer A showed a higher cytotoxicity with anti-Ig + C or anti-mu + C than the others; layer D showed the lowest % cytotoxicity, although a large % of the cells not killed by anti-Ig were killed by anti-bursa serum + C. The C + D layers contained a much larger fraction of the Ig+ cells from the bursa in the 1--4-week-old than in the 6--12-week-old animals, suggesting a relative shift towards lower density of Ig+ bursa cells with age. When [3H]adenosine-labeled bursa cells from density gradient fractions were transferred, the % injected radioactivity found in recipient spleens was higher for fractions A and B than for fraction C + D. While 50--60 % of the cells from fractions A and B which localized in spleen were found in germinal centers and follicles, a much lower % of the cells from fraction C + D seen in spleen localized in these areas. The results were in agreement with those of previous studies suggesting that it is primarily the Ig-bearing population among bursa cells which localizes in follicles."}
{"id": "PMID:1086788", "title": "Demonstration that IgG memory is carried by IgG-bearing cells.", "content": "Memory B cells which give rise to IgG antibody-producing cells were generally assumed to be IgG-bearing cells. However, recent studies indicating that very few IgG-bearing cells exist in lymphoid tissue brought this assumption into question. In this study, we examined directly the question whether IgG-bearing cells contain functional precursors of IgG antibody-producing cells. Using the adoptive secondary immune response, we demonstrated that Ig-1 b-bearing cells, isolated with the fluorescence-activated cell sorter (FACS), are the functional precursors of Ig- 1 b-producing cells. Further, we have enriched IgG2 and IgG1 memory B cells using the FACS. The results show that IgG2-bearing cells are the functional precursors of the IgG2 antibody-producing cells. Likewise, the IgG1-bearing cells are the functional precursors of IgG1 antibody-producing cells. Thus, IgG memory cells have surface IgG which indicates the class and allotype commitment of the memory cell and its progeny antibody-forming cells.", "contents": "Demonstration that IgG memory is carried by IgG-bearing cells. Memory B cells which give rise to IgG antibody-producing cells were generally assumed to be IgG-bearing cells. However, recent studies indicating that very few IgG-bearing cells exist in lymphoid tissue brought this assumption into question. In this study, we examined directly the question whether IgG-bearing cells contain functional precursors of IgG antibody-producing cells. Using the adoptive secondary immune response, we demonstrated that Ig-1 b-bearing cells, isolated with the fluorescence-activated cell sorter (FACS), are the functional precursors of Ig- 1 b-producing cells. Further, we have enriched IgG2 and IgG1 memory B cells using the FACS. The results show that IgG2-bearing cells are the functional precursors of the IgG2 antibody-producing cells. Likewise, the IgG1-bearing cells are the functional precursors of IgG1 antibody-producing cells. Thus, IgG memory cells have surface IgG which indicates the class and allotype commitment of the memory cell and its progeny antibody-forming cells."}
{"id": "PMID:1086789", "title": "Lack of neonatal susceptibility to induction of tolerance by polysaccharide antigens.", "content": "Susceptibility to tolerance induction by polysaccharides during the neonatal period has been studied with the alpha-1.3 and alpha-1.6 glucosyl epitopes of dextran B1355 in BALB/c mice and the beta-2.6 fructosyl epitope of levan in CBA mice. Acquisition of responsiveness, as measured by plaque-forming cell (PFC) assays, is relatively late - taking more than 14 days to appear and 2 - 3 months to attain maturity in the case of alpha-1.6 glucosyl and beta-2.6 fructosy. The mice responded well to sheep red blood cells and 2,4-dinitrophenylated (DNP) keyhole limpet hemocyanin (KLH) by 14 days, but were refractory to another thymus-independent antigen DNP-Ficoll. Nonresponsiveness of 2-week-old spleen cells to the polysaccharides was stable on transfer and could not be attributed to suppressor cells. Despite this long post-natal phase of immaturity, no evidence was obtained of concomitant susceptibility to tolerance induction by textran and levan. Response curves in mice injected at birth with weight-adjusted doses revealed similar or even higher \"high-zone\" thresholds to those tolerized at 3 months. Only partial alpha-1.3 glucosyl tolerance is inducible in adults but this was no greater after neonatal exposure, which led also to short-lived alpha-1.6 tolerance. Repeated injections of B1355 and levan during the first 10 days was no more tolerogenic and PFC appeared spontaneously with maturity in mice given these antigens neonatally. Thus, the recognized neonatal susceptibility to thymus-dependent antigens does not extend to these thymus-independent antigens. It is therefore considered that tolerance studied with polysaccharides has little relevance to the mechanism of self-tolerance acquired in the embryo and, in vivo, is determined by interaction with a relatively mature B cell rather than by \"clonal abortion\" of a tolerance-sensitive precursor stage.", "contents": "Lack of neonatal susceptibility to induction of tolerance by polysaccharide antigens. Susceptibility to tolerance induction by polysaccharides during the neonatal period has been studied with the alpha-1.3 and alpha-1.6 glucosyl epitopes of dextran B1355 in BALB/c mice and the beta-2.6 fructosyl epitope of levan in CBA mice. Acquisition of responsiveness, as measured by plaque-forming cell (PFC) assays, is relatively late - taking more than 14 days to appear and 2 - 3 months to attain maturity in the case of alpha-1.6 glucosyl and beta-2.6 fructosy. The mice responded well to sheep red blood cells and 2,4-dinitrophenylated (DNP) keyhole limpet hemocyanin (KLH) by 14 days, but were refractory to another thymus-independent antigen DNP-Ficoll. Nonresponsiveness of 2-week-old spleen cells to the polysaccharides was stable on transfer and could not be attributed to suppressor cells. Despite this long post-natal phase of immaturity, no evidence was obtained of concomitant susceptibility to tolerance induction by textran and levan. Response curves in mice injected at birth with weight-adjusted doses revealed similar or even higher \"high-zone\" thresholds to those tolerized at 3 months. Only partial alpha-1.3 glucosyl tolerance is inducible in adults but this was no greater after neonatal exposure, which led also to short-lived alpha-1.6 tolerance. Repeated injections of B1355 and levan during the first 10 days was no more tolerogenic and PFC appeared spontaneously with maturity in mice given these antigens neonatally. Thus, the recognized neonatal susceptibility to thymus-dependent antigens does not extend to these thymus-independent antigens. It is therefore considered that tolerance studied with polysaccharides has little relevance to the mechanism of self-tolerance acquired in the embryo and, in vivo, is determined by interaction with a relatively mature B cell rather than by \"clonal abortion\" of a tolerance-sensitive precursor stage."}
{"id": "PMID:1086790", "title": "Human T cell receptor for IgM: specificity for the pentameric Fc fragment.", "content": "The specificity of the IgM receptor expressed by human T cells cultured in IgM-free media has been investigated. IgM receptors have been detected using a rosette system with ox erythrocytes coated with rabbit antibody (EA(IgM)), and the inhibitory capacity of different IgM fragments in the rosette system has been tested. It was found that F(c)5mu but not F(ab')2mu, nor monomeric IgM (8 S IgM) inhibited EA (IgM) rosette-forming cells. This indicates that the receptor present on the surface of T cells has affinity for a structure located in the Fc portion of the pentameric IgM.", "contents": "Human T cell receptor for IgM: specificity for the pentameric Fc fragment. The specificity of the IgM receptor expressed by human T cells cultured in IgM-free media has been investigated. IgM receptors have been detected using a rosette system with ox erythrocytes coated with rabbit antibody (EA(IgM)), and the inhibitory capacity of different IgM fragments in the rosette system has been tested. It was found that F(c)5mu but not F(ab')2mu, nor monomeric IgM (8 S IgM) inhibited EA (IgM) rosette-forming cells. This indicates that the receptor present on the surface of T cells has affinity for a structure located in the Fc portion of the pentameric IgM."}
{"id": "PMID:1086791", "title": "Immunosuppressive activity of Phaseolus coccineus and Phaseolus vulgaris extracts in mice.", "content": "Lectin mixtures from 10 samples of Phaseolus coccineus and Phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (SRBC) antibody response of the mouse. Some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin A and phytohemagglutinin tested before in the same immune system. High dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the titers and \"specificities\" did not correlate with the immunosuppressive potency and selectivity displayed by the same extracts in mice. Two immunosuppressive bean extracts, assayed in lymphocyte cultures, were found to stimulate DNA synthesis of murine spleen T cells. This interaction appears to be a necessary step for the reduction of antibody synthesis to SRBC in the whole animal.", "contents": "Immunosuppressive activity of Phaseolus coccineus and Phaseolus vulgaris extracts in mice. Lectin mixtures from 10 samples of Phaseolus coccineus and Phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (SRBC) antibody response of the mouse. Some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin A and phytohemagglutinin tested before in the same immune system. High dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the titers and \"specificities\" did not correlate with the immunosuppressive potency and selectivity displayed by the same extracts in mice. Two immunosuppressive bean extracts, assayed in lymphocyte cultures, were found to stimulate DNA synthesis of murine spleen T cells. This interaction appears to be a necessary step for the reduction of antibody synthesis to SRBC in the whole animal."}
{"id": "PMID:1086792", "title": "Inhibition by mouse serum of hemopoietic colony formation in vitro.", "content": "The inhibitory activity of BALB/c serum for granulocytic and macrophage colony formation by mouse marrow cells in vitro was separable by flotation centrifugation into the very light density (VLD) fraction. On gel filtration the inhibitory material had an apparent molecular weight of 250,000 and on electrophoresis was localized as a single peak near the albumin region. These inhibitory preparations exhibited lack of specificity and inhibited colony formation by normal B-lymphocytes, lymphoid leukemic, plasma cytoma and mastocytoma cells. Preincubation of C57BL marrow cells at 37 degrees C with high concentrations of VLD fractions was cytotoxic and completely suppressed colony formation. Incubation at 0 degrees C or at 37 degrees C with low VLD concentrations caused a pattern of colony formation similar to that observed in cultures of marrow cells from mice with high serum inhibitory activity (CBA, BALC/c). The lack of specificity of serum inhibitors casts doubt on the role of these inhibitors as specific in vivo regulators of granulopoiesis and monocyte-macrophage formation.", "contents": "Inhibition by mouse serum of hemopoietic colony formation in vitro. The inhibitory activity of BALB/c serum for granulocytic and macrophage colony formation by mouse marrow cells in vitro was separable by flotation centrifugation into the very light density (VLD) fraction. On gel filtration the inhibitory material had an apparent molecular weight of 250,000 and on electrophoresis was localized as a single peak near the albumin region. These inhibitory preparations exhibited lack of specificity and inhibited colony formation by normal B-lymphocytes, lymphoid leukemic, plasma cytoma and mastocytoma cells. Preincubation of C57BL marrow cells at 37 degrees C with high concentrations of VLD fractions was cytotoxic and completely suppressed colony formation. Incubation at 0 degrees C or at 37 degrees C with low VLD concentrations caused a pattern of colony formation similar to that observed in cultures of marrow cells from mice with high serum inhibitory activity (CBA, BALC/c). The lack of specificity of serum inhibitors casts doubt on the role of these inhibitors as specific in vivo regulators of granulopoiesis and monocyte-macrophage formation."}
{"id": "PMID:1086793", "title": "Tonic influence of the efferent vestibular system on the spontaneous afferent activity from semicircular canals in the frog (Rana esculenta L.).", "content": "In the frog we have recorded the spontaneous activity of single afferent fibres of the ampullary nerves of the left horizontal semicircular canal (HC) and vertical anterior canal (VAC) in isolated head preparations. The recordings have been made in 4 experimental situations: intact preparations; preparations whose brain was destroyed; preparations whose contralateral vestibular nerve had been cut between Scarpa's ganglion and the brain; preparations where either an ampullary nerve (that of the HC or of the VAC) or the utricular nerve had been cut contralaterally. From our results, it appears that the efferent vestibular system has a tonic influence on the afferent activity from HC and VAC; the influence of the part of the efferent vestibular system (EVS) activity depending on contralateral vestibular inputs is inhibitory, while the influence of the part of the EVS activity depending on ipsilateral vestibular inputs might be facilitatory.", "contents": "Tonic influence of the efferent vestibular system on the spontaneous afferent activity from semicircular canals in the frog (Rana esculenta L.). In the frog we have recorded the spontaneous activity of single afferent fibres of the ampullary nerves of the left horizontal semicircular canal (HC) and vertical anterior canal (VAC) in isolated head preparations. The recordings have been made in 4 experimental situations: intact preparations; preparations whose brain was destroyed; preparations whose contralateral vestibular nerve had been cut between Scarpa's ganglion and the brain; preparations where either an ampullary nerve (that of the HC or of the VAC) or the utricular nerve had been cut contralaterally. From our results, it appears that the efferent vestibular system has a tonic influence on the afferent activity from HC and VAC; the influence of the part of the efferent vestibular system (EVS) activity depending on contralateral vestibular inputs is inhibitory, while the influence of the part of the EVS activity depending on ipsilateral vestibular inputs might be facilitatory."}
{"id": "PMID:1086794", "title": "Levels of alpha1-antitrypsin in the spring and autumn seasons.", "content": "In a group of 84 pairs of 11-year-old children of both sexes, the level of the alpha1-antitrypsin (alpha1-AT) were ascertained in the autumn and spring. Although the mean levels of alpha1-AT in the two seasons hardly differed, the highly significant seasonal changes in the distribution curves of alpha1-AT values were noted in boys, whereas the levels showed higher stability in girls.", "contents": "Levels of alpha1-antitrypsin in the spring and autumn seasons. In a group of 84 pairs of 11-year-old children of both sexes, the level of the alpha1-antitrypsin (alpha1-AT) were ascertained in the autumn and spring. Although the mean levels of alpha1-AT in the two seasons hardly differed, the highly significant seasonal changes in the distribution curves of alpha1-AT values were noted in boys, whereas the levels showed higher stability in girls."}
{"id": "PMID:1086795", "title": "The loss of biological activity of 5-hydroxytryptamine creatinine sulphate.", "content": "5-Hydroxytryptamine creatinine sulphate loses its biological activity when maintained at room temperature. The loss of 5-HT activity (in stimulating sodium transport across frog skin) is greater than the loss of creatinine sulphate activity (inhibition of sodium transport).", "contents": "The loss of biological activity of 5-hydroxytryptamine creatinine sulphate. 5-Hydroxytryptamine creatinine sulphate loses its biological activity when maintained at room temperature. The loss of 5-HT activity (in stimulating sodium transport across frog skin) is greater than the loss of creatinine sulphate activity (inhibition of sodium transport)."}
{"id": "PMID:1086796", "title": "Depletion of synaptic vesicles at the frog (Rana pipiens) neuromuscular junctions by tetraphenylboron.", "content": "At the frog neuromuscular junction tetraphenylboron produced a decrease in miniature endplate potential amplitude, accompanied by a decrease in the number but not the size of synaptic vesicles.", "contents": "Depletion of synaptic vesicles at the frog (Rana pipiens) neuromuscular junctions by tetraphenylboron. At the frog neuromuscular junction tetraphenylboron produced a decrease in miniature endplate potential amplitude, accompanied by a decrease in the number but not the size of synaptic vesicles."}
{"id": "PMID:1086797", "title": "Does an initial phasic response exist in the receptor potential of taste cells?", "content": "The depolarizing receptor potentials to 0.5 M NaCl recorded from frog taste cells did not exhibit any phasic response, even when the rectangular waveform of stimulus onset was employed. The quickest depolarizations recorded reached the peak in 50 msec. On the other hand, the gustatory neural response showed initial overshoot of the impulse discharge even when 0.5 M NaCl was delivered at the slower rate of 0.06 ml/sec. It is concluded that the initial neural response may be associated with the rate of rise of the receptor potential before its plateau level is reached.", "contents": "Does an initial phasic response exist in the receptor potential of taste cells? The depolarizing receptor potentials to 0.5 M NaCl recorded from frog taste cells did not exhibit any phasic response, even when the rectangular waveform of stimulus onset was employed. The quickest depolarizations recorded reached the peak in 50 msec. On the other hand, the gustatory neural response showed initial overshoot of the impulse discharge even when 0.5 M NaCl was delivered at the slower rate of 0.06 ml/sec. It is concluded that the initial neural response may be associated with the rate of rise of the receptor potential before its plateau level is reached."}
{"id": "PMID:1086801", "title": "[Oriented rat movement in a rotating system].", "content": "In experiments with rotating of rats, their avoidance responce was studied: rats moved into the central portion. The 7 degrees or 15 degrees pitch of the central portion's walls caused significant moving of rats towards a square peripheral portion. When experimenting in darkness, the moving--towards--center response was restored, while under illumination the preference of the peripheral portion was again revealed in the rats obtained are are regarded as confirming the hypothesis of taht the moving--to center response during rotation is due to discrepancy between the vestibular and the visual systems of coordinates in peripheral portions of centrifuge.", "contents": "[Oriented rat movement in a rotating system]. In experiments with rotating of rats, their avoidance responce was studied: rats moved into the central portion. The 7 degrees or 15 degrees pitch of the central portion's walls caused significant moving of rats towards a square peripheral portion. When experimenting in darkness, the moving--towards--center response was restored, while under illumination the preference of the peripheral portion was again revealed in the rats obtained are are regarded as confirming the hypothesis of taht the moving--to center response during rotation is due to discrepancy between the vestibular and the visual systems of coordinates in peripheral portions of centrifuge."}
{"id": "PMID:1086802", "title": "[Inhibitory influence on the heart of antidromal stimulation of afferent fibers of the vagus nerve].", "content": "In partially or completely deparasympathized frogs, inhibition was not potentiated by the specific inhibitor of \"true\" cholinesterase acetylcholinesterase: the 1,5-bis(4-tri-methylammoniumphenylpeptan-3-on-diiodide). The localization of cardiac \"true cholinesterase\" being determined only in the region of the postsynaptic membrane of post-ganglionic neurons, this enzyme lacking on their extrasynaptic surface and in the myocardium, it was concluded that excitatory effect of antidromic afferent activation on the intracardiac neurons was due to action of acetylcholine (released from the nerve endings) on the extrasynaptic region of neurons' membrane. These regions acquire cholino-receptive features after preganglionic parasympathetic degeneration.", "contents": "[Inhibitory influence on the heart of antidromal stimulation of afferent fibers of the vagus nerve]. In partially or completely deparasympathized frogs, inhibition was not potentiated by the specific inhibitor of \"true\" cholinesterase acetylcholinesterase: the 1,5-bis(4-tri-methylammoniumphenylpeptan-3-on-diiodide). The localization of cardiac \"true cholinesterase\" being determined only in the region of the postsynaptic membrane of post-ganglionic neurons, this enzyme lacking on their extrasynaptic surface and in the myocardium, it was concluded that excitatory effect of antidromic afferent activation on the intracardiac neurons was due to action of acetylcholine (released from the nerve endings) on the extrasynaptic region of neurons' membrane. These regions acquire cholino-receptive features after preganglionic parasympathetic degeneration."}
{"id": "PMID:1086803", "title": "[Relationship between the strength of myocardial fiber contraction of frog heart ventricle and processes of intracellular energy transport].", "content": "At 10--15 mM of creatine in the perfusate the force of the myocardium contraction was increased. At concentrations over 40 mM, creatine causes a decrease of contractile force which can be significantly increased after washing-out of the excess of creatine. Prolonged perfusion (over 10 hrs) with Ringer's solution leads to a decrease in intracellular creatine concentration and to a parallel decrease of contractile force. Under these conditions the intracellular concentration of creatine phosphate is significantly decreased with small changes in ATP content. The addition of creatine to perfusate leads to the complete restoration of contractile force and creatine phosphate content in heart strips' cells. These results indicate that the contractile force of heart strips depends upon high energy phosphate content (mainly creatine-phosphate content) in the cells and are consistent with the idea of the existence of the creatinephosphate pathway of energy transport in heart cells.", "contents": "[Relationship between the strength of myocardial fiber contraction of frog heart ventricle and processes of intracellular energy transport]. At 10--15 mM of creatine in the perfusate the force of the myocardium contraction was increased. At concentrations over 40 mM, creatine causes a decrease of contractile force which can be significantly increased after washing-out of the excess of creatine. Prolonged perfusion (over 10 hrs) with Ringer's solution leads to a decrease in intracellular creatine concentration and to a parallel decrease of contractile force. Under these conditions the intracellular concentration of creatine phosphate is significantly decreased with small changes in ATP content. The addition of creatine to perfusate leads to the complete restoration of contractile force and creatine phosphate content in heart strips' cells. These results indicate that the contractile force of heart strips depends upon high energy phosphate content (mainly creatine-phosphate content) in the cells and are consistent with the idea of the existence of the creatinephosphate pathway of energy transport in heart cells."}
{"id": "PMID:1086804", "title": "Electrokinetic properties of splenic lymphocytes from the low-lipopolysaccharide responder C3H/Hej mice.", "content": "Spleen cells from C3H/Hej mice (H-2k) respond poorly to the B-cell mitogen lipopolysaccharide in vitro as compared to the related strains C3H/Tif (H-2k) and CBA/Orl (H-2k). The electrokinetic properties of splenic lymphocytes from these 3 strains were investigated in parallel, in order to both quantitate low-mobility B cell and high-mobility T cell populations and measure their mean electrophoretic mobilities. C3H/Hej mice were found to possess the same proportion (55%) of LM cells as C3H/Tif and CBA/Orl mice. Therefore, the low LPS-responsiveness of C3H/Hej is not due to a numerical deficiency in B cells. Whereas the mean EPM of HM cells was identical in the 3 strains, that of LM cells was slightly (6%) but significantly (Student's t test, P less than 0.01) lower in C3H/Hej than in the high LPS-responder controls. This suggests that the membrane-structure required for activating interaction with LPS might contribute to B-cell electronegative surface-charge.", "contents": "Electrokinetic properties of splenic lymphocytes from the low-lipopolysaccharide responder C3H/Hej mice. Spleen cells from C3H/Hej mice (H-2k) respond poorly to the B-cell mitogen lipopolysaccharide in vitro as compared to the related strains C3H/Tif (H-2k) and CBA/Orl (H-2k). The electrokinetic properties of splenic lymphocytes from these 3 strains were investigated in parallel, in order to both quantitate low-mobility B cell and high-mobility T cell populations and measure their mean electrophoretic mobilities. C3H/Hej mice were found to possess the same proportion (55%) of LM cells as C3H/Tif and CBA/Orl mice. Therefore, the low LPS-responsiveness of C3H/Hej is not due to a numerical deficiency in B cells. Whereas the mean EPM of HM cells was identical in the 3 strains, that of LM cells was slightly (6%) but significantly (Student's t test, P less than 0.01) lower in C3H/Hej than in the high LPS-responder controls. This suggests that the membrane-structure required for activating interaction with LPS might contribute to B-cell electronegative surface-charge."}
{"id": "PMID:1086805", "title": "Changes in agglutination of cells with H-2 antibodies or lectins and in stimulation of lymphocytes as induced by certain polymers (chondroitin sulphate/PVP/PHA/Con A/capping of H-2 antigens).", "content": "The effect of certain polymers on the course of immune interaction has been well known although its mechanism at the cellular level remains obscure. We thus affected cells in vitro with two of such substances, CS and PVP, and followed their behaviour in various assays. It was shown that in this way the agglutinability of mouse erythrocytes with H-2 antibodies or of mouse lymphocytes with threshold doses of Con A could be enhanced, antibody-induced capping of H-2 antigens on lymphocytes accelerated and PHA-induced stimulation of mouse lymphocytes inhibited. The possible mechanisms of these changes putatively mediated through the effect on the cell membrane or cell coat are discussed.", "contents": "Changes in agglutination of cells with H-2 antibodies or lectins and in stimulation of lymphocytes as induced by certain polymers (chondroitin sulphate/PVP/PHA/Con A/capping of H-2 antigens). The effect of certain polymers on the course of immune interaction has been well known although its mechanism at the cellular level remains obscure. We thus affected cells in vitro with two of such substances, CS and PVP, and followed their behaviour in various assays. It was shown that in this way the agglutinability of mouse erythrocytes with H-2 antibodies or of mouse lymphocytes with threshold doses of Con A could be enhanced, antibody-induced capping of H-2 antigens on lymphocytes accelerated and PHA-induced stimulation of mouse lymphocytes inhibited. The possible mechanisms of these changes putatively mediated through the effect on the cell membrane or cell coat are discussed."}
{"id": "PMID:1086808", "title": "Aspects of lymphocyte function in Beh\u00e7et's disease.", "content": "Aspects of lymphocyte function were investigated, in vitro, in nine patients with active Beh\u00e7et's disease. The rosette formation technique revealed a slight decrease in number of T lymphocytes and more so of B lymphocytes. In spite of their low number, the transformation of the T lymphocytes in response to mitogens did not show a significant difference from that of normal controls. This may indicate a hyperreactivity, and in two instances a spontaneous blastogenesis prior to addition of mitogens, was also noted. The immunoglobulins and complement levels in serum were within normal limits.", "contents": "Aspects of lymphocyte function in Beh\u00e7et's disease. Aspects of lymphocyte function were investigated, in vitro, in nine patients with active Beh\u00e7et's disease. The rosette formation technique revealed a slight decrease in number of T lymphocytes and more so of B lymphocytes. In spite of their low number, the transformation of the T lymphocytes in response to mitogens did not show a significant difference from that of normal controls. This may indicate a hyperreactivity, and in two instances a spontaneous blastogenesis prior to addition of mitogens, was also noted. The immunoglobulins and complement levels in serum were within normal limits."}
{"id": "PMID:1086811", "title": "Hemangiomas of the cecum. Colonoscopic diagnosis and therapy.", "content": "Five cases of hemangiomas of the cecum and gastrointestinal bleeding are presented. All were demonstrated by colonscopy after more conventional diagnostic methods failed. The hemangiomas were bright red, flat lesions clearly seen through the colonscope. They were successfully treated by electrocoagulation. All patients in this series had some type of associated cardiac or vascular disease. These patients may represent a syndrome of gastrointestinal blood loss of obscure cause, hemangiomas of the cecum, and cardiovascular disease.", "contents": "Hemangiomas of the cecum. Colonoscopic diagnosis and therapy. Five cases of hemangiomas of the cecum and gastrointestinal bleeding are presented. All were demonstrated by colonscopy after more conventional diagnostic methods failed. The hemangiomas were bright red, flat lesions clearly seen through the colonscope. They were successfully treated by electrocoagulation. All patients in this series had some type of associated cardiac or vascular disease. These patients may represent a syndrome of gastrointestinal blood loss of obscure cause, hemangiomas of the cecum, and cardiovascular disease."}
{"id": "PMID:1086810", "title": "[Evaluation of the agglutinating action of lectins on Trichomonas vaginalis by means of different methods].", "content": "Trichomonas vaginalis (strain FC) are specifically agglutinated by Con A and PHA-P but not by WGA. The techniques used gave basically the same results however the agglutination in plates has the advantage of a good sensitivity and a greater simplicity of execution.", "contents": "[Evaluation of the agglutinating action of lectins on Trichomonas vaginalis by means of different methods]. Trichomonas vaginalis (strain FC) are specifically agglutinated by Con A and PHA-P but not by WGA. The techniques used gave basically the same results however the agglutination in plates has the advantage of a good sensitivity and a greater simplicity of execution."}
{"id": "PMID:1086815", "title": "[Capacity of activation of so-called deficient factors in hereditary blood coagulation disorders].", "content": "By factor-specific exchange-experiments it was proved that the lack of factor VIII activity in hemophilia A is not caused by an incapacity of activation or even lack of the factor, but by a defect of the pertaining activator resp. activator system. The same goes for factor IX in hemophilia B as well as factors VII, IX and X in family defect of the prothrombin-complex. In contrast, inactivity in the von Willebrand-J\u00fcnrgens-disease is obviously caused by a genuine lack of factor VIII.", "contents": "[Capacity of activation of so-called deficient factors in hereditary blood coagulation disorders]. By factor-specific exchange-experiments it was proved that the lack of factor VIII activity in hemophilia A is not caused by an incapacity of activation or even lack of the factor, but by a defect of the pertaining activator resp. activator system. The same goes for factor IX in hemophilia B as well as factors VII, IX and X in family defect of the prothrombin-complex. In contrast, inactivity in the von Willebrand-J\u00fcnrgens-disease is obviously caused by a genuine lack of factor VIII."}
{"id": "PMID:1086816", "title": "[Hemorrhagic enteropathy].", "content": "Intestinal infarction in the absence of organic vascular occlusion received increasing attention in recent years. The clinical picture is discussed based on results in 9 cases, an attempt to suggest a possible pathophysiological mechanism is made. Prophylactic digitalisation especially in the elderly patient in the absence of severe heart failure and in cases with already low mesenteric perfusion may lead to a further vasoconstriction and to hemorrhagic enteropathy. Therapeutic possibilities are discussed.", "contents": "[Hemorrhagic enteropathy]. Intestinal infarction in the absence of organic vascular occlusion received increasing attention in recent years. The clinical picture is discussed based on results in 9 cases, an attempt to suggest a possible pathophysiological mechanism is made. Prophylactic digitalisation especially in the elderly patient in the absence of severe heart failure and in cases with already low mesenteric perfusion may lead to a further vasoconstriction and to hemorrhagic enteropathy. Therapeutic possibilities are discussed."}
{"id": "PMID:1086817", "title": "[Clinical importance of Meckel's diverticulum].", "content": "Between 1966 und 1975 42 children and 46 adults were operated on Meckel's diverticulum. The diverticulum is explained as one of the possible disturbances during regression of ductus omphaloentericus. The appendicitis-like symptomatology correlates to the involvement of gastric and colonic mucosa as well as heterotopic exo- and endocrine tissue of the pancreas in the wall of the diverticulum. In 34% complications were due to inflammation, perforation, bleeding, intussusception, volvulus, gut-strangulation with ileus, ulcer, neoplasia or lesion by foreign bodies. 4 out of 88 patients died. Preoperative diagnostic fails in 75%, therefore in every case the distal gut should be inspected and every diverticulum should be resected.", "contents": "[Clinical importance of Meckel's diverticulum]. Between 1966 und 1975 42 children and 46 adults were operated on Meckel's diverticulum. The diverticulum is explained as one of the possible disturbances during regression of ductus omphaloentericus. The appendicitis-like symptomatology correlates to the involvement of gastric and colonic mucosa as well as heterotopic exo- and endocrine tissue of the pancreas in the wall of the diverticulum. In 34% complications were due to inflammation, perforation, bleeding, intussusception, volvulus, gut-strangulation with ileus, ulcer, neoplasia or lesion by foreign bodies. 4 out of 88 patients died. Preoperative diagnostic fails in 75%, therefore in every case the distal gut should be inspected and every diverticulum should be resected."}
{"id": "PMID:1086818", "title": "[Diagnostic and therapeutic measures in acute catastrophic bleeding esophageal varices].", "content": "Principles of management of bleeding esophageal varices are 1. fluid therapy of bleeding shock, 2. prevention of hepatic coma, 3. emergency endoscopy, 4. balloon tubes (Senkstaken-Blakemore, Linton-Nachlas), and 5. with some restriction, selective infusion of vasopressin into the a. mesenterica superior. If these procedures fail, sclerosing of esophageal varices stops bleeding in more than 90% of the cases. Bleeding from varices of the gastric fundus may be stopped by gastro-esophageal disconnection (Pettinari-Hassab). Both procedures have with 15% and 25% respectively, the lowest mortality. Patients for surgical shunt are carefully selected within the interval after bleeding. Shunts are the distal splenal-renal and the mesenteric-caval anastomosis with dacron prothesis (H-shunt). The shunt is the favorable therapy for prehepatic block in patients older than 14 to 16 years. The endoscopic sclerosing of esophageal varices and the gastro-esophageal disconnection are chosen in younger patients or when shunt procedures are not possible. The posthepatic block is treated successfully by conservative means. In most cases, surgical therapy is contraindicated because of poor prognosis. When conservative measures fail, in few cases emergency endoscopic sclerosing of esophageal varices or latero-lateral porto-caval anastomosis can be tried.", "contents": "[Diagnostic and therapeutic measures in acute catastrophic bleeding esophageal varices]. Principles of management of bleeding esophageal varices are 1. fluid therapy of bleeding shock, 2. prevention of hepatic coma, 3. emergency endoscopy, 4. balloon tubes (Senkstaken-Blakemore, Linton-Nachlas), and 5. with some restriction, selective infusion of vasopressin into the a. mesenterica superior. If these procedures fail, sclerosing of esophageal varices stops bleeding in more than 90% of the cases. Bleeding from varices of the gastric fundus may be stopped by gastro-esophageal disconnection (Pettinari-Hassab). Both procedures have with 15% and 25% respectively, the lowest mortality. Patients for surgical shunt are carefully selected within the interval after bleeding. Shunts are the distal splenal-renal and the mesenteric-caval anastomosis with dacron prothesis (H-shunt). The shunt is the favorable therapy for prehepatic block in patients older than 14 to 16 years. The endoscopic sclerosing of esophageal varices and the gastro-esophageal disconnection are chosen in younger patients or when shunt procedures are not possible. The posthepatic block is treated successfully by conservative means. In most cases, surgical therapy is contraindicated because of poor prognosis. When conservative measures fail, in few cases emergency endoscopic sclerosing of esophageal varices or latero-lateral porto-caval anastomosis can be tried."}
{"id": "PMID:1086822", "title": "Elastases from human and canine granulocytes, I. Some proteolytic and esterolytic properties.", "content": "The lysosome-like granules of human and canine granulocytes contain an enzyme with elastinolytic activity. The enzymatic behaviour of these elastases was further characterized using the protein substrates elastin-orcein and azocasein and the synthetic substrates tert.-butyloxycarbonyl-alanine p-nitrophenylester (Boc-Ala-ONp) and 3-carboxypropionyl-L-alanyl-L-alanyl-L-alanine p-nitroanilide (Suc-Ala3-NHNp) in photometric assays. The affinities of the granulocyte elastases and of porcine pancreatic elastase to these substrates are very similar, e.g. human granulocyte elastase: KM (Boc-Ala-ONp) = 0.35mM, KM (Suc-Ala3-NHNp) = 1.25mM, porcine pancreatic elastase: KM (Boc-Ala-ONp) = 0.3mM, KM (Suc-Ala3-NHNp) - 1.15mM. The most convenient substrate for the assay of human and dog granulocyte elastases and for kinetic measurements with these enzymes is Suc-Ala3-NHNp. Using this substrate, the dissociation constant of the complex of human granulocyte elastase with human alpha1-antitrypsin could be determined (Ki = 3.5 x 10(-10)M).", "contents": "Elastases from human and canine granulocytes, I. Some proteolytic and esterolytic properties. The lysosome-like granules of human and canine granulocytes contain an enzyme with elastinolytic activity. The enzymatic behaviour of these elastases was further characterized using the protein substrates elastin-orcein and azocasein and the synthetic substrates tert.-butyloxycarbonyl-alanine p-nitrophenylester (Boc-Ala-ONp) and 3-carboxypropionyl-L-alanyl-L-alanyl-L-alanine p-nitroanilide (Suc-Ala3-NHNp) in photometric assays. The affinities of the granulocyte elastases and of porcine pancreatic elastase to these substrates are very similar, e.g. human granulocyte elastase: KM (Boc-Ala-ONp) = 0.35mM, KM (Suc-Ala3-NHNp) = 1.25mM, porcine pancreatic elastase: KM (Boc-Ala-ONp) = 0.3mM, KM (Suc-Ala3-NHNp) - 1.15mM. The most convenient substrate for the assay of human and dog granulocyte elastases and for kinetic measurements with these enzymes is Suc-Ala3-NHNp. Using this substrate, the dissociation constant of the complex of human granulocyte elastase with human alpha1-antitrypsin could be determined (Ki = 3.5 x 10(-10)M)."}
{"id": "PMID:1086824", "title": "The function of T cells carrying receptors for complexes of Ig and antigen.", "content": "Thymocytes exposed briefly in vitro to a variety of particulate substances (such as mycobacteria, erythrocytes of allogeneic cells) or to substances known to act in vivo as adjuvants (LPS or poly A:U), generate supernates which are able to induce cytophilic Ig in normal mouse serum in the presence of a foreign protein (antigen). This cytophilic Ig is taken up by 20-25% of splenic T cells. Hydrocortisone resistant thymocytes show the same property, while bone marrow cells are inactive. This activity is similar to that reported previously as being present in the 4S fraction of mouse serum, collected 6 hours after injection of complete Freund's adjuvant. It is proposed that this factor is responsible for the formation of complexes of Ig and antigen which have been detected in the serum 6 hours after immunization. Thymocytes collected 6 hours after priming in vivo with SRBC (when a subpopulation among them carries easily demonstrable surface Ig) are able to amplify markedly the antibody response particularly the 7S. It is postulated that the factor by generating the cytophilic Ig (complexes?) which is taken up by T cells, sets up a mechanism which markedly amplifies their helper cell function.", "contents": "The function of T cells carrying receptors for complexes of Ig and antigen. Thymocytes exposed briefly in vitro to a variety of particulate substances (such as mycobacteria, erythrocytes of allogeneic cells) or to substances known to act in vivo as adjuvants (LPS or poly A:U), generate supernates which are able to induce cytophilic Ig in normal mouse serum in the presence of a foreign protein (antigen). This cytophilic Ig is taken up by 20-25% of splenic T cells. Hydrocortisone resistant thymocytes show the same property, while bone marrow cells are inactive. This activity is similar to that reported previously as being present in the 4S fraction of mouse serum, collected 6 hours after injection of complete Freund's adjuvant. It is proposed that this factor is responsible for the formation of complexes of Ig and antigen which have been detected in the serum 6 hours after immunization. Thymocytes collected 6 hours after priming in vivo with SRBC (when a subpopulation among them carries easily demonstrable surface Ig) are able to amplify markedly the antibody response particularly the 7S. It is postulated that the factor by generating the cytophilic Ig (complexes?) which is taken up by T cells, sets up a mechanism which markedly amplifies their helper cell function."}
{"id": "PMID:1086825", "title": "Competitive inhibition of passive sensitization of mouse mast cells by IgE. A bioassay for mouse and rat IgE.", "content": "Possibility of inhibition of an efficient in vitro IgE-sensitization system was studied. The sentization of mouse peritoneal mast cells with an anti-ovalbumin IgE-rich fraction of serum, as tested by ovalbumin-induced degranulation, was inhibited by previous incubation with antisera of another or of no specificity. Fractionation and other experiments showed that the inhibiting activity correlated with IgE content. IgGl did not seem to have an effect. Sensitization was also inhibited by rat myeloma IgE, 50 ng giving a 50 per cent inhibition. Plots of the logarithms of rat and mouse IgE concentration vs their inhibitory effect on sensitization gave two parallel linear curves, indicating that mouse and rat IgE compete for the same receptor sites. It was thus possible to use this system as a sensitive bioassay for both mouse and rat IgE levels and, by comparing inhibition by mouse IgE to that by a known rat IgE standard, to obtain not only relative data but absolute mouse IgE levels. This, and also a better discrimination of IgE doses, was the major advantage of this bioassay in relation to the equally sensitive anti-IgE degranulation tests.", "contents": "Competitive inhibition of passive sensitization of mouse mast cells by IgE. A bioassay for mouse and rat IgE. Possibility of inhibition of an efficient in vitro IgE-sensitization system was studied. The sentization of mouse peritoneal mast cells with an anti-ovalbumin IgE-rich fraction of serum, as tested by ovalbumin-induced degranulation, was inhibited by previous incubation with antisera of another or of no specificity. Fractionation and other experiments showed that the inhibiting activity correlated with IgE content. IgGl did not seem to have an effect. Sensitization was also inhibited by rat myeloma IgE, 50 ng giving a 50 per cent inhibition. Plots of the logarithms of rat and mouse IgE concentration vs their inhibitory effect on sensitization gave two parallel linear curves, indicating that mouse and rat IgE compete for the same receptor sites. It was thus possible to use this system as a sensitive bioassay for both mouse and rat IgE levels and, by comparing inhibition by mouse IgE to that by a known rat IgE standard, to obtain not only relative data but absolute mouse IgE levels. This, and also a better discrimination of IgE doses, was the major advantage of this bioassay in relation to the equally sensitive anti-IgE degranulation tests."}
{"id": "PMID:1086826", "title": "Study of human T and B lymphocytes with heterologous antisera. I Preparation, specificity and properties of antisera.", "content": "Heterologous antisera have been raised in the horse and rabbit against human lymphocytes. Appropriate absorptions on either B or T cells were performed to make antisera specific for human T (anti-HTLA) or B (serum 789) lymphocytes respectively. In addition serum 789 was found to react with circulating monocytes. The percentages of T and B cells detected by anti-HTLA and 789 sera in the different lymphoid organs averaged respectively: 78-7 per cent and 14-7 per cent in peripheral blood, 91-4 per cent and 4-0 per cent in thymus, 73-0 per cent and 14-5 per cent in lymph nodes, 53-6 per cent and 30-0 per cent in spleen, 47-1 per cent and 47-6 per cent in tonsils and 17-5 per cent and 13-5 per cent in bone marrow. Anti-HTLA serum appeared to supress E-rosette formation but did not affect binding of C3-coated erythrocytes. Serum 789 did not prevent E-rosette formation and reduced the number of EAC rosettes by 50 per cent. Anti-HTLA serum was found able to suti-lymphocyte serum, and PWM in the presence of complement; it was found highly mitogenic by itself. Serum 789 decreased the proliferative response to phytomitogens in about the proportion of cells killed by the antiserum. These results indicate that the presence of T cells is necessary for the mitogen-induced proliferation to occur, and that B lymphocytes are induced to proliferate in the presence of T cells and phytomitogens. Anti-HTLA serum was found not to inhibit K-cell activity of lymphocytes against antibody-coated chicken erythrocytes. These antisera appear very useful tools for the study of the role of human B and T lymphocytes involved in in vitro immune reactions.", "contents": "Study of human T and B lymphocytes with heterologous antisera. I Preparation, specificity and properties of antisera. Heterologous antisera have been raised in the horse and rabbit against human lymphocytes. Appropriate absorptions on either B or T cells were performed to make antisera specific for human T (anti-HTLA) or B (serum 789) lymphocytes respectively. In addition serum 789 was found to react with circulating monocytes. The percentages of T and B cells detected by anti-HTLA and 789 sera in the different lymphoid organs averaged respectively: 78-7 per cent and 14-7 per cent in peripheral blood, 91-4 per cent and 4-0 per cent in thymus, 73-0 per cent and 14-5 per cent in lymph nodes, 53-6 per cent and 30-0 per cent in spleen, 47-1 per cent and 47-6 per cent in tonsils and 17-5 per cent and 13-5 per cent in bone marrow. Anti-HTLA serum appeared to supress E-rosette formation but did not affect binding of C3-coated erythrocytes. Serum 789 did not prevent E-rosette formation and reduced the number of EAC rosettes by 50 per cent. Anti-HTLA serum was found able to suti-lymphocyte serum, and PWM in the presence of complement; it was found highly mitogenic by itself. Serum 789 decreased the proliferative response to phytomitogens in about the proportion of cells killed by the antiserum. These results indicate that the presence of T cells is necessary for the mitogen-induced proliferation to occur, and that B lymphocytes are induced to proliferate in the presence of T cells and phytomitogens. Anti-HTLA serum was found not to inhibit K-cell activity of lymphocytes against antibody-coated chicken erythrocytes. These antisera appear very useful tools for the study of the role of human B and T lymphocytes involved in in vitro immune reactions."}
{"id": "PMID:1086827", "title": "Activation and suppression of T cells in termination of immunological tolerance.", "content": "Immunological tolerance was induced in adult C3H mice by five injections of ultra-centrifuged, soluble HSA at weekly intervals. The tolerant state was readily terminated by the challenge immunization with slightly sulphanilated HSA administered as Freund's complete adjuvant emulsion. In this case, it was shown that T cells specific for HSA were activated which could serve as helpers for the production of anti-hapten antibodies. The tolerance termination and activation of T cells specific for HSA was not observed in the profound tolerant state, induced by nine injections of ultra-centrifuged, soluble HSA at weekly intervals, where suppressor T cells were detected. Implications of these results were discussed in reference to the development of autoimmunity.", "contents": "Activation and suppression of T cells in termination of immunological tolerance. Immunological tolerance was induced in adult C3H mice by five injections of ultra-centrifuged, soluble HSA at weekly intervals. The tolerant state was readily terminated by the challenge immunization with slightly sulphanilated HSA administered as Freund's complete adjuvant emulsion. In this case, it was shown that T cells specific for HSA were activated which could serve as helpers for the production of anti-hapten antibodies. The tolerance termination and activation of T cells specific for HSA was not observed in the profound tolerant state, induced by nine injections of ultra-centrifuged, soluble HSA at weekly intervals, where suppressor T cells were detected. Implications of these results were discussed in reference to the development of autoimmunity."}
{"id": "PMID:1086828", "title": "Rapid adsorption of a foetal calf serum component by mammalian cells in culture. A potential source of artifacts in studies of antisera to cell-specific antigens.", "content": "Injection of CBA mice the either mitogen-stimulated (LPS or con A) CBA lymphocytes which had been cultured for 3 days in the presence of foetal calf serum (FCS) led to the production of antisera which reacted strongly with virtually all types of mammalian cells, including human, whether normal or malignant, provided they had been cultured in FCS-containing media. Reactivity was detected by sensitive immunological assays such as complement-dependent cytoxocity using rabbit complement (but not using guinea-pig complement), or EA-rosette inhibition of Fc receptor-bearing cells. The antisera did not react with fresh normal lymphoid cells or ascites tumour cells; however, these same cell populations became fully susceptible to the cytotoxic effects of the antisera after as little as 4 h incubation at 37 degrees in the presence of FCS. Cells incubated without FCS or with FCS at 0 degrees were not affected. The antisera reacted with FCS to form a single band on Ouchterlony double-diffusion plates. On immunoelectrophoresis the reactive antigen appeared to migrate in the alpha-globulin region of serum proteins. These observations suggest that FCS may be a source of potentially serious misinterpretations in immunological studies of cell-associated antigens using antisera produced by the injection of cells grown in FCS-containing cultures. Examples of artifcats arising from the use of FCS in certain systems, e.g. the preparation of alloantisera using cultured tumour cells vs fresh non-cultured lymphoid cells, are described.", "contents": "Rapid adsorption of a foetal calf serum component by mammalian cells in culture. A potential source of artifacts in studies of antisera to cell-specific antigens. Injection of CBA mice the either mitogen-stimulated (LPS or con A) CBA lymphocytes which had been cultured for 3 days in the presence of foetal calf serum (FCS) led to the production of antisera which reacted strongly with virtually all types of mammalian cells, including human, whether normal or malignant, provided they had been cultured in FCS-containing media. Reactivity was detected by sensitive immunological assays such as complement-dependent cytoxocity using rabbit complement (but not using guinea-pig complement), or EA-rosette inhibition of Fc receptor-bearing cells. The antisera did not react with fresh normal lymphoid cells or ascites tumour cells; however, these same cell populations became fully susceptible to the cytotoxic effects of the antisera after as little as 4 h incubation at 37 degrees in the presence of FCS. Cells incubated without FCS or with FCS at 0 degrees were not affected. The antisera reacted with FCS to form a single band on Ouchterlony double-diffusion plates. On immunoelectrophoresis the reactive antigen appeared to migrate in the alpha-globulin region of serum proteins. These observations suggest that FCS may be a source of potentially serious misinterpretations in immunological studies of cell-associated antigens using antisera produced by the injection of cells grown in FCS-containing cultures. Examples of artifcats arising from the use of FCS in certain systems, e.g. the preparation of alloantisera using cultured tumour cells vs fresh non-cultured lymphoid cells, are described."}
{"id": "PMID:1086829", "title": "An IgG thymolytic autoantibody in rats which has specificity for a subpopulation of T cells.", "content": "A cytotoxic anti-thymocyte IgG auto-antibody is present in Lewis rats which, in the presence of autologous complement, destroys (in vitro) 12-28 per cent of isologous or autologous thymocytes, a smaller number of lymph node cells and splenocytes, but not bone marrow or circulating lymphocytes. The labile cells in the thymus represent a finite subpopulation which is autologous antithymocyte antibody (ATS) sensitive and steroid resistant. The presence of the autoantibody is randomly distributed in outbred animals whereas in inbred Lewis rats, a strain in which the induction of some autoimmune reactions is under genetic control, the antibody is always present. In this strain, the susceptible T cells and the quantity of circulating autoantibody is significantly depressed during the productive phase of a T-cell mediated disease (adjuvant polyarthritis) and returns to normal after the disease becomes stabilized. There is a direct relationship between the amount of susceptible cells in the thymus and the amount of antibody in circulation, suggesting that the antibody could serve as a marker for a specific subpopulation of thymocytes which may have a regulatory influence on T-cell reactivity.", "contents": "An IgG thymolytic autoantibody in rats which has specificity for a subpopulation of T cells. A cytotoxic anti-thymocyte IgG auto-antibody is present in Lewis rats which, in the presence of autologous complement, destroys (in vitro) 12-28 per cent of isologous or autologous thymocytes, a smaller number of lymph node cells and splenocytes, but not bone marrow or circulating lymphocytes. The labile cells in the thymus represent a finite subpopulation which is autologous antithymocyte antibody (ATS) sensitive and steroid resistant. The presence of the autoantibody is randomly distributed in outbred animals whereas in inbred Lewis rats, a strain in which the induction of some autoimmune reactions is under genetic control, the antibody is always present. In this strain, the susceptible T cells and the quantity of circulating autoantibody is significantly depressed during the productive phase of a T-cell mediated disease (adjuvant polyarthritis) and returns to normal after the disease becomes stabilized. There is a direct relationship between the amount of susceptible cells in the thymus and the amount of antibody in circulation, suggesting that the antibody could serve as a marker for a specific subpopulation of thymocytes which may have a regulatory influence on T-cell reactivity."}
{"id": "PMID:1086831", "title": "Experimental endogenous endophthalmitis caused by Haemophilus influenzae type b.", "content": "Experimental endogenous endophthalmitis was produced in infant rats by either intranasal or intraperitoneal inoculation with Haemophilus influenzae type b and 5 days of age. The ocular disease occurred in about 50% of bacteremic animals who survived to age 12 days and probably represents metastatic bacterial infection secondary to hematogenous seeding. The lesion was a highly destructive suppurative endophthalmitis that ultimately progressed to panophthalmitis and was followed by organization of the exudate and phthisis bulbi.", "contents": "Experimental endogenous endophthalmitis caused by Haemophilus influenzae type b. Experimental endogenous endophthalmitis was produced in infant rats by either intranasal or intraperitoneal inoculation with Haemophilus influenzae type b and 5 days of age. The ocular disease occurred in about 50% of bacteremic animals who survived to age 12 days and probably represents metastatic bacterial infection secondary to hematogenous seeding. The lesion was a highly destructive suppurative endophthalmitis that ultimately progressed to panophthalmitis and was followed by organization of the exudate and phthisis bulbi."}
{"id": "PMID:1086832", "title": "Participation of complement in the nonimmune host defense against experimental Haemophilus influenzae type b septicemia and meningitis.", "content": "This study was undertaken to determine whether the terminal complement components (C3-9) are involved in the nonimmune host defense against Haemophilus influenzae type b septicemia and meningitis. Using cobra venom factor, infant rats were depleted of C3 and C5. After intranasal challenge with H. influenzae type b, the complement-depleted rats developed a greater incidence and magnitude of bacteremia and a higher mortality rate. In contrast to the effects on bacteremia, complement depletion did not directly influence either the occurrence of meningitis or bacterial multiplication within the cerebrospinal fluid. These experiments provide evidence that the complement system may be an important mechanism of natural immunity to H. influenzae type b.", "contents": "Participation of complement in the nonimmune host defense against experimental Haemophilus influenzae type b septicemia and meningitis. This study was undertaken to determine whether the terminal complement components (C3-9) are involved in the nonimmune host defense against Haemophilus influenzae type b septicemia and meningitis. Using cobra venom factor, infant rats were depleted of C3 and C5. After intranasal challenge with H. influenzae type b, the complement-depleted rats developed a greater incidence and magnitude of bacteremia and a higher mortality rate. In contrast to the effects on bacteremia, complement depletion did not directly influence either the occurrence of meningitis or bacterial multiplication within the cerebrospinal fluid. These experiments provide evidence that the complement system may be an important mechanism of natural immunity to H. influenzae type b."}
{"id": "PMID:1086833", "title": "A comparison of three health status indicators.", "content": "Interest in health status indicators has produced measures of widely varying applicability. We were interested in the use of such indices to establish mean recovery curves for groups of similar patients undergoing acute hospital treatment. A longitudinal study relating resource usage to these recovery curves had been intended but there were difficulties in finding suitable indicators. Firstly, two published indicators relying on patient interviews were tested for consistency. Poor correlations were found among those scorers unfamiliar with the patients and it seems unlikely that these indicators could be used in a routine system. Different parts of the indices presented difficulties to the different professions involved in scoring, and a multidisciplinary approach may be needed in assessing full health. The indicators tested included no assessment of prognosis. Those parts of the indices which had produced significant correlations were retained in subsequent work and were supplemented by further measures designed to overcome the earlier difficulties. A new trial of this indicator was undertaken where staff, familiar with the patients, scored data recorded by the Problem Oriented Medical Record system. This produced improved correlations but some problems remain.", "contents": "A comparison of three health status indicators. Interest in health status indicators has produced measures of widely varying applicability. We were interested in the use of such indices to establish mean recovery curves for groups of similar patients undergoing acute hospital treatment. A longitudinal study relating resource usage to these recovery curves had been intended but there were difficulties in finding suitable indicators. Firstly, two published indicators relying on patient interviews were tested for consistency. Poor correlations were found among those scorers unfamiliar with the patients and it seems unlikely that these indicators could be used in a routine system. Different parts of the indices presented difficulties to the different professions involved in scoring, and a multidisciplinary approach may be needed in assessing full health. The indicators tested included no assessment of prognosis. Those parts of the indices which had produced significant correlations were retained in subsequent work and were supplemented by further measures designed to overcome the earlier difficulties. A new trial of this indicator was undertaken where staff, familiar with the patients, scored data recorded by the Problem Oriented Medical Record system. This produced improved correlations but some problems remain."}
{"id": "PMID:1086834", "title": "The Tokelau Island migrant study: prevalence of various conditions before migration.", "content": "The Tokelau Island Migrant Study has shown no important differences between those who subsequently left their home islands to migrate to New Zealand and those who remained, in key anthropometric and biochemical variables already reported. This comparison is now extended to various common diseases and conditions, and again no major difference emerges. The Tokelauans are compared with other Polynesians and shown to have less diabetes, hypertension, effort pain, chronic bronchitis and varicose veins than New Zealand Maoris, while resembling some Cook Island groups. Changes in prevalences of some conditions following migration are postulated.", "contents": "The Tokelau Island migrant study: prevalence of various conditions before migration. The Tokelau Island Migrant Study has shown no important differences between those who subsequently left their home islands to migrate to New Zealand and those who remained, in key anthropometric and biochemical variables already reported. This comparison is now extended to various common diseases and conditions, and again no major difference emerges. The Tokelauans are compared with other Polynesians and shown to have less diabetes, hypertension, effort pain, chronic bronchitis and varicose veins than New Zealand Maoris, while resembling some Cook Island groups. Changes in prevalences of some conditions following migration are postulated."}
{"id": "PMID:1086835", "title": "Regeneration and dose-response characteristics of irradiated mouse dorsal epidermal cells.", "content": "A microcolony technique is described for measuring epidermal cell survival 3 days after whole-body X-irradiation. This assay provides a cell D0 value of 233 +/- 11 rad and a zero-dose extrapolate of 1-23 x 10(4) cells/cm2 for mice irradiated in oxygen 20 hours after hair plucking. The microcolony cellularity had an apparent doubling-time of 25 hours which may be an upper limit at least for some clones. The clones appeared to fragment continually and form new daughter clones, suggesting that few would form macroscopic nodules. Many of the clones were also apparently associated with hair follicles.", "contents": "Regeneration and dose-response characteristics of irradiated mouse dorsal epidermal cells. A microcolony technique is described for measuring epidermal cell survival 3 days after whole-body X-irradiation. This assay provides a cell D0 value of 233 +/- 11 rad and a zero-dose extrapolate of 1-23 x 10(4) cells/cm2 for mice irradiated in oxygen 20 hours after hair plucking. The microcolony cellularity had an apparent doubling-time of 25 hours which may be an upper limit at least for some clones. The clones appeared to fragment continually and form new daughter clones, suggesting that few would form macroscopic nodules. Many of the clones were also apparently associated with hair follicles."}
{"id": "PMID:1086836", "title": "Effect of 2 MHz ultrasound on DNA, RNA and protein synthesis in Pisum sativum root meristem cells.", "content": "The amounts of DNA, RNA and protein synthesis were determined in Pisum sativum root meristem cells at various times after a 1 min exposure to 1 MHz ultrasound at a power density of 30 W/cm2. Immediate depressions in all three macromolecular syntheses occurred after sonication, followed by an apparent recovery several hours later. These events appear to correlate in time with the subsequent reduction and recovery in mitotic index in Pisum sativum root meristem cells exposed to 2 MHz ultrasound.", "contents": "Effect of 2 MHz ultrasound on DNA, RNA and protein synthesis in Pisum sativum root meristem cells. The amounts of DNA, RNA and protein synthesis were determined in Pisum sativum root meristem cells at various times after a 1 min exposure to 1 MHz ultrasound at a power density of 30 W/cm2. Immediate depressions in all three macromolecular syntheses occurred after sonication, followed by an apparent recovery several hours later. These events appear to correlate in time with the subsequent reduction and recovery in mitotic index in Pisum sativum root meristem cells exposed to 2 MHz ultrasound."}
{"id": "PMID:1086837", "title": "Biological relevance of gamma-ray-induced alkali-labile sites in single-stranded Dna in aqueous solutions.", "content": "Gamma-irradiation in single-stranded phiX174 DNA in aqueous solution in the presence of oxygen produces at least two types of alkali-labile site. One is lethal and gives rise to single-strand breaks by treatment with alkali. The other is non-lethal, but is converted to lethal damage by alkali. The effect of alkali is dependent on temperature. This dependence is different for both types of alkali-labile site.", "contents": "Biological relevance of gamma-ray-induced alkali-labile sites in single-stranded Dna in aqueous solutions. Gamma-irradiation in single-stranded phiX174 DNA in aqueous solution in the presence of oxygen produces at least two types of alkali-labile site. One is lethal and gives rise to single-strand breaks by treatment with alkali. The other is non-lethal, but is converted to lethal damage by alkali. The effect of alkali is dependent on temperature. This dependence is different for both types of alkali-labile site."}
{"id": "PMID:1086838", "title": "X-irradiation of equine peripheral blood lymphocytes stimulated with phytohaemagglutinin in vitro.", "content": "Small lymphocytes were isolated from the peripheral blood of horses and incubated at 37 degrees C in Eagle's medium supplemented with 20 per cent foetal calf serum. The addition of phytohaemagglutinin (PHA) to the cultures resulted in: increased RNA and protein synthesis; the enlargement of the small lymphocyte into a lymphoblast-like cell; the initiation of DNA synthesis, and cell division. When survival was measured 24 hours after X-irradiation by means of phase-contrast microscopy, the lymphoblast-like cell was much more radio-resistant (D0 = 250 rad) than the small lymphocyte (D0 = 20 rad). This increase in radioresistance, however, was not observed until 12-24 hours after PHA treatment. To investigate which of the changes occurring during the transformation of the small lymphocyte was responsible for the increased resistance to irradiation, the percentage of cells surviving irradiation was compared with the percentage of cells incorporating significant amounts of 3HTdR, 3H-UR, or 3H-leucine at the time of irradiation. For this comparison, a dose of 100 rad was used because 100 rad killed essentially all of the small lymphocytes, but less than 35 percent of the cells which had become radioresistant from the PHA treatment. The results indicated that the increase in radioresistance was not associated with DNA synthesis, but instead correlated with the increase in RNA and protein synthesis which the cells had attained at the time of irradiation.", "contents": "X-irradiation of equine peripheral blood lymphocytes stimulated with phytohaemagglutinin in vitro. Small lymphocytes were isolated from the peripheral blood of horses and incubated at 37 degrees C in Eagle's medium supplemented with 20 per cent foetal calf serum. The addition of phytohaemagglutinin (PHA) to the cultures resulted in: increased RNA and protein synthesis; the enlargement of the small lymphocyte into a lymphoblast-like cell; the initiation of DNA synthesis, and cell division. When survival was measured 24 hours after X-irradiation by means of phase-contrast microscopy, the lymphoblast-like cell was much more radio-resistant (D0 = 250 rad) than the small lymphocyte (D0 = 20 rad). This increase in radioresistance, however, was not observed until 12-24 hours after PHA treatment. To investigate which of the changes occurring during the transformation of the small lymphocyte was responsible for the increased resistance to irradiation, the percentage of cells surviving irradiation was compared with the percentage of cells incorporating significant amounts of 3HTdR, 3H-UR, or 3H-leucine at the time of irradiation. For this comparison, a dose of 100 rad was used because 100 rad killed essentially all of the small lymphocytes, but less than 35 percent of the cells which had become radioresistant from the PHA treatment. The results indicated that the increase in radioresistance was not associated with DNA synthesis, but instead correlated with the increase in RNA and protein synthesis which the cells had attained at the time of irradiation."}
{"id": "PMID:1086839", "title": "The effect of nitroimidazole and nitroxyl radiosensitizers on the post-irradiation synthesis of DNA.", "content": "The modification of DNA damage by three radiosensitizing drugs, present during gamma-irradiation of hypoxic Chinese hamster cells, was investigated. Both 2-methyl-5-nitroimidazole-1-ethanol (metronidazole) and 1-(2-nitro-1-imidazole)-3-methoxy-2-propranol (Ro-07-0582) were found to cause large increases in the yield of DNA single-strand breaks (SSB); triacetoneamine-N-oxyl (TAN) was found to have only a small effect on SSB production. The three drugs tested did not inhibit the rejoining of SSB. A pulse label and chase procedure was used to examine post-irradiation DNA synthesis. TAN present during irradiation under hypoxia was found to cause interruptions in subsequent DNA synthesis. Metronidazole and Ro-07-0582 had no effect on post-irradiation DNA synthesis. In addition, the effects of pre- and post-irradiation exposure to TAN were investigated, since these treatments have shown increased cell-killing in survival studies. TAN pre- and post-treatments were found to have no significant effect on subsequent DNA synthesis.", "contents": "The effect of nitroimidazole and nitroxyl radiosensitizers on the post-irradiation synthesis of DNA. The modification of DNA damage by three radiosensitizing drugs, present during gamma-irradiation of hypoxic Chinese hamster cells, was investigated. Both 2-methyl-5-nitroimidazole-1-ethanol (metronidazole) and 1-(2-nitro-1-imidazole)-3-methoxy-2-propranol (Ro-07-0582) were found to cause large increases in the yield of DNA single-strand breaks (SSB); triacetoneamine-N-oxyl (TAN) was found to have only a small effect on SSB production. The three drugs tested did not inhibit the rejoining of SSB. A pulse label and chase procedure was used to examine post-irradiation DNA synthesis. TAN present during irradiation under hypoxia was found to cause interruptions in subsequent DNA synthesis. Metronidazole and Ro-07-0582 had no effect on post-irradiation DNA synthesis. In addition, the effects of pre- and post-irradiation exposure to TAN were investigated, since these treatments have shown increased cell-killing in survival studies. TAN pre- and post-treatments were found to have no significant effect on subsequent DNA synthesis."}
{"id": "PMID:1086840", "title": "Viable hybrids between lethally X-irradiated hamster cells and unirradiated mouse cells.", "content": "Senda\u00ef-virus-induced fusion between heavily X-irradiated hamster cells, BHK21 or RS2-3 (BHK21 cells transformed by Rous Sarcoma Virus), and unirradiated mouse cells, A9 or c11D, give rise to hybrids. These hybrids possess mouse and hamster surface antigens. However, RS2-3 x mouse hybrids do not form heterokaryons with chick-embryo fibroblasts producing infectious Rous sarcoma virus.", "contents": "Viable hybrids between lethally X-irradiated hamster cells and unirradiated mouse cells. Senda\u00ef-virus-induced fusion between heavily X-irradiated hamster cells, BHK21 or RS2-3 (BHK21 cells transformed by Rous Sarcoma Virus), and unirradiated mouse cells, A9 or c11D, give rise to hybrids. These hybrids possess mouse and hamster surface antigens. However, RS2-3 x mouse hybrids do not form heterokaryons with chick-embryo fibroblasts producing infectious Rous sarcoma virus."}
{"id": "PMID:1086843", "title": "R.b.e. and o.e.r. of extended-Bragg-peak helium ions: survival and development of rat embryos.", "content": "Rats were exposed under aerobic or hypoxic conditions to 200-1200 rads of 60Co gamma-rays or extended-Bragg-peak helium ions on the eighth day of gestation. Uterine contents were examined on the twentieth day of gestation. At the 50 per cent embryonic survival level, helium ion r.b.e. was 1(.0) (aerobic) and 1(.2) (hypoxic). Maximum attainable gamma-ray and helium-ion o.e.r.s. were 2(.2) and 1(.7) respectively, indicating an oxygen-effect gain (o.e.g.) of 1(.2). At the 10 per cent survival level helium ion r.b.e. was 1(.1) (aerobic) and 1(.4) (hypoxic). Gamma-ray and helium-ion 0.e.r.s. were 2(.0) and 1(.5) respectively, indicating a helium ion o.e.g. of 1(.3). These data demonstrate that the small fraction of high-LET radiation present in this helium ion beam has a neglible effect on the aerobic r.b.e., but lowers the effective o.e.r. of the beam approximately 25 per cent relative to that of gamma-rays. Helium ions were significantly more effective than gamma-rays in killing embryos under hypoxic conditions, in producing congenital abnormalities under aerobic conditions, and in stunting foetal growth under both conditions.", "contents": "R.b.e. and o.e.r. of extended-Bragg-peak helium ions: survival and development of rat embryos. Rats were exposed under aerobic or hypoxic conditions to 200-1200 rads of 60Co gamma-rays or extended-Bragg-peak helium ions on the eighth day of gestation. Uterine contents were examined on the twentieth day of gestation. At the 50 per cent embryonic survival level, helium ion r.b.e. was 1(.0) (aerobic) and 1(.2) (hypoxic). Maximum attainable gamma-ray and helium-ion o.e.r.s. were 2(.2) and 1(.7) respectively, indicating an oxygen-effect gain (o.e.g.) of 1(.2). At the 10 per cent survival level helium ion r.b.e. was 1(.1) (aerobic) and 1(.4) (hypoxic). Gamma-ray and helium-ion 0.e.r.s. were 2(.0) and 1(.5) respectively, indicating a helium ion o.e.g. of 1(.3). These data demonstrate that the small fraction of high-LET radiation present in this helium ion beam has a neglible effect on the aerobic r.b.e., but lowers the effective o.e.r. of the beam approximately 25 per cent relative to that of gamma-rays. Helium ions were significantly more effective than gamma-rays in killing embryos under hypoxic conditions, in producing congenital abnormalities under aerobic conditions, and in stunting foetal growth under both conditions."}
{"id": "PMID:1086844", "title": "Effects of electrons and neutrons on the synthesis of RNA in resistant and sensitive strains of the slime-mould Dictyostelium discoideum and the modifying effect of caffeine.", "content": "RNA synthesis was investigated after irradiation in resistant and sensitive lines of the slime-mould Dictyostelium discoideum. When 3H adenine was used as a precursor to RNA, incorporation increased after irradiation in the resistant WT line but not in the sensitive line (gammas-13). The extent of RNA synthesis after irradiation was correlated with the shoulder width on the survival curve of the resistant line. When this was reduced by irradiating with neutrons, or treatment with caffeine RNA synthesis was also reduced. No preferential synthesis of one RNA species occurred; there was increased labelling in all RNA species after irradiation. Sucrose gradient analysis of ribosomal RNA extracted from irradiated cells and free of messenger RNA revealed no apparent difference in composition from that extracted from unirradiated cells. Increased RNA synthesis after irradiation may form part of the recovery processin the resistant cells.", "contents": "Effects of electrons and neutrons on the synthesis of RNA in resistant and sensitive strains of the slime-mould Dictyostelium discoideum and the modifying effect of caffeine. RNA synthesis was investigated after irradiation in resistant and sensitive lines of the slime-mould Dictyostelium discoideum. When 3H adenine was used as a precursor to RNA, incorporation increased after irradiation in the resistant WT line but not in the sensitive line (gammas-13). The extent of RNA synthesis after irradiation was correlated with the shoulder width on the survival curve of the resistant line. When this was reduced by irradiating with neutrons, or treatment with caffeine RNA synthesis was also reduced. No preferential synthesis of one RNA species occurred; there was increased labelling in all RNA species after irradiation. Sucrose gradient analysis of ribosomal RNA extracted from irradiated cells and free of messenger RNA revealed no apparent difference in composition from that extracted from unirradiated cells. Increased RNA synthesis after irradiation may form part of the recovery processin the resistant cells."}
{"id": "PMID:1086845", "title": "Analysis of the time relationship for the interaction of X-ray induced primary breaks in the formation of dicentric chromosomes.", "content": "In split-dose experiments, the time relationship for the interaction of primary breaks in the formation of dicentric chromosomes was analysed. Human peripheral lymphocytes were irradiated with a dose of 340 R of 220 kV X-rays split into two equal fractions separated by intervals up to 360 min. Assuming an exponential decline of the time-dependent quadratic component of the dose realtion of dicentrics a theoretical formula was deduced. Fitting this formula to the empirical data a mean time of 110 min could be calculated in which primary breaks induced by both dose-fractions can interact to form dicentric chromosomes.", "contents": "Analysis of the time relationship for the interaction of X-ray induced primary breaks in the formation of dicentric chromosomes. In split-dose experiments, the time relationship for the interaction of primary breaks in the formation of dicentric chromosomes was analysed. Human peripheral lymphocytes were irradiated with a dose of 340 R of 220 kV X-rays split into two equal fractions separated by intervals up to 360 min. Assuming an exponential decline of the time-dependent quadratic component of the dose realtion of dicentrics a theoretical formula was deduced. Fitting this formula to the empirical data a mean time of 110 min could be calculated in which primary breaks induced by both dose-fractions can interact to form dicentric chromosomes."}
{"id": "PMID:1086846", "title": "Abscopal effect of irradiation on haemopoietic stem cells of shielded bone marrow--role of migration.", "content": "Immediately after partial-body irradiation, the number of colony-forming units (CFU) of the protected marrow decreases. This decrease seems to be dose-dependent and cannot be completely explained by migration for the following reasons: there is no evidence of CFU entry to irradiated territories and the number of circulating CFU does not increase. A more plausible explanation for the phenomena is an accelerated differentiation during the first 15 min after irradiation. The migration of CFU to the spleen is observed between 15 min and 3 hours after irradiation, after which it ceases. The absence of migration 3 hours after irradiation may be due to one of the two possibilities: (1) a depletion of the mobile pool of CFU if one admits that such a pool is responsible for migration in the organism; (2) a depletion of a temporarily increased stimulus due to irradiation.", "contents": "Abscopal effect of irradiation on haemopoietic stem cells of shielded bone marrow--role of migration. Immediately after partial-body irradiation, the number of colony-forming units (CFU) of the protected marrow decreases. This decrease seems to be dose-dependent and cannot be completely explained by migration for the following reasons: there is no evidence of CFU entry to irradiated territories and the number of circulating CFU does not increase. A more plausible explanation for the phenomena is an accelerated differentiation during the first 15 min after irradiation. The migration of CFU to the spleen is observed between 15 min and 3 hours after irradiation, after which it ceases. The absence of migration 3 hours after irradiation may be due to one of the two possibilities: (1) a depletion of the mobile pool of CFU if one admits that such a pool is responsible for migration in the organism; (2) a depletion of a temporarily increased stimulus due to irradiation."}
{"id": "PMID:1086847", "title": "Vasoformative non-osteogenic (angio) sarcomas of bone-marrow stroma due to strontium-90.", "content": "In a series of experiments, mainly CBA/H, but also C2H/H, mice aged 3 months were injected intraperitoneally with solutions of 90Sr Cl2, the dose per mouse varying from 7 to 20 muCi, and compared with similar mice treated with 226Ra or 239Pu, discussed elsewhere. In male mice, the commonest tumour resulting at each dose of 90Sr was non-osteogenic (angio) sarcoma, a tumour not seen after 226Ra. In females, this tumour occurred far less frequently than osteosarcoma. In CBA mice of both sexes converted to radiation chimaeras (which are sterile) and similarly treated with 90Sr, the only skeletal tumours were osteosarcomas. When only half the body of CBA mice was X-irradiated with 1000 rad and the mice given 90Sr, non-osteogenic sarcoma occurred predominantly in those mice X-irradiated in the cephalic half. The results suggest that intact testes may provide co-factors for this type of neoplasm, whereas others have shown that oestrogens facilitate murine osteosarcoma. The non-osteogenic osteosarcomas arise from damaged stromal elements in bone-marrow of selected bones. The risk to this component of bone-marrow, as well as to haematopoietic tissue, should be considered in radiation protection.", "contents": "Vasoformative non-osteogenic (angio) sarcomas of bone-marrow stroma due to strontium-90. In a series of experiments, mainly CBA/H, but also C2H/H, mice aged 3 months were injected intraperitoneally with solutions of 90Sr Cl2, the dose per mouse varying from 7 to 20 muCi, and compared with similar mice treated with 226Ra or 239Pu, discussed elsewhere. In male mice, the commonest tumour resulting at each dose of 90Sr was non-osteogenic (angio) sarcoma, a tumour not seen after 226Ra. In females, this tumour occurred far less frequently than osteosarcoma. In CBA mice of both sexes converted to radiation chimaeras (which are sterile) and similarly treated with 90Sr, the only skeletal tumours were osteosarcomas. When only half the body of CBA mice was X-irradiated with 1000 rad and the mice given 90Sr, non-osteogenic sarcoma occurred predominantly in those mice X-irradiated in the cephalic half. The results suggest that intact testes may provide co-factors for this type of neoplasm, whereas others have shown that oestrogens facilitate murine osteosarcoma. The non-osteogenic osteosarcomas arise from damaged stromal elements in bone-marrow of selected bones. The risk to this component of bone-marrow, as well as to haematopoietic tissue, should be considered in radiation protection."}
{"id": "PMID:1086849", "title": "Inhibition of prostaglandin-mediated ocular inflammatory responses by 4-biphenylacetic acid.", "content": "4-Biphenylacetic acid (BPAA),a prostaglandin-synthesis inhibitor, was tested for its effects on prostaglandin-related, laboratory models of ocular inflammation. Topically applied, BPAA inhibited arachidonic acid, but not prostaglandin E-induced increases in rabbit intraocular pressure (IOP). BPAA inhibited the IOP response to alkali burn and altered IOP changes following paracentesis. In vitro, BPAA inhibited prostaglandin production from arachidonic acid in cell-free preparations of rabbit uvea. It is suggested that BPAA may be useful for the therapy of ocular inflammatory disease.", "contents": "Inhibition of prostaglandin-mediated ocular inflammatory responses by 4-biphenylacetic acid. 4-Biphenylacetic acid (BPAA),a prostaglandin-synthesis inhibitor, was tested for its effects on prostaglandin-related, laboratory models of ocular inflammation. Topically applied, BPAA inhibited arachidonic acid, but not prostaglandin E-induced increases in rabbit intraocular pressure (IOP). BPAA inhibited the IOP response to alkali burn and altered IOP changes following paracentesis. In vitro, BPAA inhibited prostaglandin production from arachidonic acid in cell-free preparations of rabbit uvea. It is suggested that BPAA may be useful for the therapy of ocular inflammatory disease."}
{"id": "PMID:1086850", "title": "[Chronic vegetating pyodermia in cellular immune deficiency].", "content": "The simultaneous occurrence of pyoderma gangrenosum together with other diseases, most frequently ulcerative colitis, and with disorders of the humoral immune system is known. The etiology of the individual case remains nevertheless unclear in most instances. We report on an otherwise normal patient with normal immunoglobulin levels, who showed, by use of lymphocyte differentiation and functional tests, a nearly complete lack of T-cell reactivity. A substitution with transfusions of fresh blood lead to rapid healing of the clinical picture, which so far had been resistant to long term application of antibiotics, azathioprine and topical steroids. Therefore it is recommended to look for functional defects of the T-cell system as a cause of pyoderma gangrenosum.", "contents": "[Chronic vegetating pyodermia in cellular immune deficiency]. The simultaneous occurrence of pyoderma gangrenosum together with other diseases, most frequently ulcerative colitis, and with disorders of the humoral immune system is known. The etiology of the individual case remains nevertheless unclear in most instances. We report on an otherwise normal patient with normal immunoglobulin levels, who showed, by use of lymphocyte differentiation and functional tests, a nearly complete lack of T-cell reactivity. A substitution with transfusions of fresh blood lead to rapid healing of the clinical picture, which so far had been resistant to long term application of antibiotics, azathioprine and topical steroids. Therefore it is recommended to look for functional defects of the T-cell system as a cause of pyoderma gangrenosum."}
{"id": "PMID:1086851", "title": "Behavior in the Lesch--Nyhan syndrome.", "content": "The Lesch--Nyhan syndrome is a heritable disorder of the metabolism of uric acid in which behavioral manifestations are prominent and among the most provocative. The mutated or variant gene that determines this disorder is carried on the X chromosome. The disease is expressed exclusively in males. The molecular expression of the abnormal gene is in the completely defective activity of the enzyme hypoxanthine guanine phosphoribosyl transferase. As a result these patients overproduce uric acid and may develop early in life many of the clinical findings we associate with gout. They have in addition a variety of neurological abnormalities including mental retardation, spastic cerebral palsy, and involuntary, choreoathetoid movements. Involved patients have unusual, compulsive, aggressive behavior. Its most prominent but by no means exclusive feature is self-mutilation. The central feature in the management of this behavior is physical restraint. A number of practical procedures have been learned which facilitate the care and feeding of these patients. Promising new findings suggest that behavioral modification using extinction techniques and pharmacologic methods utilizing agents designed to increase the effective cerebral content of serotonin may each have a place in the management of behavior in this syndrome.", "contents": "Behavior in the Lesch--Nyhan syndrome. The Lesch--Nyhan syndrome is a heritable disorder of the metabolism of uric acid in which behavioral manifestations are prominent and among the most provocative. The mutated or variant gene that determines this disorder is carried on the X chromosome. The disease is expressed exclusively in males. The molecular expression of the abnormal gene is in the completely defective activity of the enzyme hypoxanthine guanine phosphoribosyl transferase. As a result these patients overproduce uric acid and may develop early in life many of the clinical findings we associate with gout. They have in addition a variety of neurological abnormalities including mental retardation, spastic cerebral palsy, and involuntary, choreoathetoid movements. Involved patients have unusual, compulsive, aggressive behavior. Its most prominent but by no means exclusive feature is self-mutilation. The central feature in the management of this behavior is physical restraint. A number of practical procedures have been learned which facilitate the care and feeding of these patients. Promising new findings suggest that behavioral modification using extinction techniques and pharmacologic methods utilizing agents designed to increase the effective cerebral content of serotonin may each have a place in the management of behavior in this syndrome."}
{"id": "PMID:1086852", "title": "Anatomic variants of the cerebello-pontine cisterns and their significance in positive contrast cisternography.", "content": "Far lateral extension of the cerebello-pontine cistern is a common variant which is found in greater than 50% of people. When present it may cause the internal auditory canal to be obscured during positive contrast cisternography. A technique of cisternography is described which facilitates the diagnosis of intracanalicular lesions as well as better delineating small lesions in the cerebello-pontine angle.", "contents": "Anatomic variants of the cerebello-pontine cisterns and their significance in positive contrast cisternography. Far lateral extension of the cerebello-pontine cistern is a common variant which is found in greater than 50% of people. When present it may cause the internal auditory canal to be obscured during positive contrast cisternography. A technique of cisternography is described which facilitates the diagnosis of intracanalicular lesions as well as better delineating small lesions in the cerebello-pontine angle."}
{"id": "PMID:1086853", "title": "Immunologic considerations of large volume radiotherapy.", "content": "There is clinical evidence that the immune response can increase or decrease cancer growth. 1) Cancer is increased in congenital immune deficiency states and in those given immuno-suppressants after renal transplantation; 2) there is a correlation between mononuclear cell infiltration and prognosis; 3) spontaneous regression occurs occasionally on an immunologic basis; 4) cancer specific antigens and antibodies can be demonstrated, and 5) immunotherapy can cause tumor regression. The cells that are responsible for the immune response are 1) families of thymic-derived lymphocytes, or T-cells, (\"memory,\" \"effector\", \"helper\" or \"suppressor\" T-cells), and 2) bursal-derived lymphocytes, or B-cells, which on specific sensitization become either plasma cells that produce cytotoxic or blocking antibody or macrophages (A-cells) that process antigen, and may become \"armed\" with cytophilic antibody. The main effect of local radiotherapy is to destroy certain families of radiosensitive lymphocytes in the afferent, central and efferent components of the immune response. In Hodgkin's disease, extended field radiotherapy causes a prolonged decrease in the number of lymphocytes in the peripheral blood and a decrease in function of the cells, as determined by RNA and DNA synthesis after culture with mitogen, phytohemagglutinin. There is no correlation, however, with the values after treatment and recurrence of the disease. The effect of local radiotherapy to large volumes of tissues, on the incidence of new primary cancers, is uncertain but the majority of reports show that it is no effect.", "contents": "Immunologic considerations of large volume radiotherapy. There is clinical evidence that the immune response can increase or decrease cancer growth. 1) Cancer is increased in congenital immune deficiency states and in those given immuno-suppressants after renal transplantation; 2) there is a correlation between mononuclear cell infiltration and prognosis; 3) spontaneous regression occurs occasionally on an immunologic basis; 4) cancer specific antigens and antibodies can be demonstrated, and 5) immunotherapy can cause tumor regression. The cells that are responsible for the immune response are 1) families of thymic-derived lymphocytes, or T-cells, (\"memory,\" \"effector\", \"helper\" or \"suppressor\" T-cells), and 2) bursal-derived lymphocytes, or B-cells, which on specific sensitization become either plasma cells that produce cytotoxic or blocking antibody or macrophages (A-cells) that process antigen, and may become \"armed\" with cytophilic antibody. The main effect of local radiotherapy is to destroy certain families of radiosensitive lymphocytes in the afferent, central and efferent components of the immune response. In Hodgkin's disease, extended field radiotherapy causes a prolonged decrease in the number of lymphocytes in the peripheral blood and a decrease in function of the cells, as determined by RNA and DNA synthesis after culture with mitogen, phytohemagglutinin. There is no correlation, however, with the values after treatment and recurrence of the disease. The effect of local radiotherapy to large volumes of tissues, on the incidence of new primary cancers, is uncertain but the majority of reports show that it is no effect."}
{"id": "PMID:1086854", "title": "\"Cleavage\" and cortical granule breakdown in Rana pipiens oocytes induced by direct microinjection of calcium.", "content": "Microinjection of approximately 0.3 mug of calcium into maturing oocytes of Rana pipiens after nuclear dissolution resulted in cleavage-like constrictions, cortical granule breakdown, and formation of a structure resembling a two-cell embryo. Mg2+, Na+, or K+ did not induce any of these reactions. Larger amounts of Ca2+-induced contraction over the entire surface of oocytes or eggs, but did not induce cleavage-like constrictions; smaller amounts of Ca2+ produced either a local cortical granule reaction of the formation of one large and one small \"blastomere.\" Furrow formation was not observed during normally induced maturation until after germinal vesicle breakdown. The location of microinjected Ca2+ determined the orientation of the resulting furrow. Ca2+-induced cortical granule breakdown occurred in full-grown nonmaturing oocytes near the site of injection. Cortical granule breakdown also occurred in maturing oocytes (after germinal vesicle breakdown but before second meiotic metaphase), but only in the blastomere containing the infected Ca2+. As expected, in mature oocytes (at second meiotic metaphase) cortical granule breakdown occurred over the entire oocyte surface, including both blastomeres. The results indicate that furrow formation and cleavage-like constrictions may be directly influenced by Ca2+, and that functional contractile elements are present near all areas of the oocyte surface. Furthermore, Ca2+ injection initiates localized cortical granule breakdown in full-grown immature and maturing oocytes.", "contents": "\"Cleavage\" and cortical granule breakdown in Rana pipiens oocytes induced by direct microinjection of calcium. Microinjection of approximately 0.3 mug of calcium into maturing oocytes of Rana pipiens after nuclear dissolution resulted in cleavage-like constrictions, cortical granule breakdown, and formation of a structure resembling a two-cell embryo. Mg2+, Na+, or K+ did not induce any of these reactions. Larger amounts of Ca2+-induced contraction over the entire surface of oocytes or eggs, but did not induce cleavage-like constrictions; smaller amounts of Ca2+ produced either a local cortical granule reaction of the formation of one large and one small \"blastomere.\" Furrow formation was not observed during normally induced maturation until after germinal vesicle breakdown. The location of microinjected Ca2+ determined the orientation of the resulting furrow. Ca2+-induced cortical granule breakdown occurred in full-grown nonmaturing oocytes near the site of injection. Cortical granule breakdown also occurred in maturing oocytes (after germinal vesicle breakdown but before second meiotic metaphase), but only in the blastomere containing the infected Ca2+. As expected, in mature oocytes (at second meiotic metaphase) cortical granule breakdown occurred over the entire oocyte surface, including both blastomeres. The results indicate that furrow formation and cleavage-like constrictions may be directly influenced by Ca2+, and that functional contractile elements are present near all areas of the oocyte surface. Furthermore, Ca2+ injection initiates localized cortical granule breakdown in full-grown immature and maturing oocytes."}
{"id": "PMID:1086855", "title": "Factors affecting pharyngeal Haemophilus influenzae type b colonization rates in children.", "content": "Over 1,300 children were studied in an analysis of factors that might affect pharyngeal colonization with Haemophilus influenzae type b. Our semiquantitative methods for the culture of H. influenzae type b, consisting of inoculation of 0.001 ml of throat swab fluid on antiserum agar plates and division of the results into three grades of intensity, showed agreement as to intensity of colonization in over 80% of repeat throat cultures. Our data also suggest that throat swabs are more efficient than nasopharyngeal swabs for detecting colonization, particularly for older children. All 17 H. influenzae type b carriers found with either method were detected with throat swabs, but six had negative nasopharyngeal cultures; four of these six were lightly colonized older children. Furthermore, colony counts were apt to be higher on plates inoculated with throat swab fluids. The frequency of pharyngeal H. influenzae type b colonization in children visiting health department clinics and pediatricians' offices was low during the first 6 months of life (0.7%) but averaged 3 to 5% throughout the rest of childhood. Approximately two-thirds of the carriers were colonized at an intensity too low to be detected by standard laboratory techniques. No influence on colonization rates was found for sex, race, season, economic status, or common childhood infectious diseases such as coryza or otitis media.", "contents": "Factors affecting pharyngeal Haemophilus influenzae type b colonization rates in children. Over 1,300 children were studied in an analysis of factors that might affect pharyngeal colonization with Haemophilus influenzae type b. Our semiquantitative methods for the culture of H. influenzae type b, consisting of inoculation of 0.001 ml of throat swab fluid on antiserum agar plates and division of the results into three grades of intensity, showed agreement as to intensity of colonization in over 80% of repeat throat cultures. Our data also suggest that throat swabs are more efficient than nasopharyngeal swabs for detecting colonization, particularly for older children. All 17 H. influenzae type b carriers found with either method were detected with throat swabs, but six had negative nasopharyngeal cultures; four of these six were lightly colonized older children. Furthermore, colony counts were apt to be higher on plates inoculated with throat swab fluids. The frequency of pharyngeal H. influenzae type b colonization in children visiting health department clinics and pediatricians' offices was low during the first 6 months of life (0.7%) but averaged 3 to 5% throughout the rest of childhood. Approximately two-thirds of the carriers were colonized at an intensity too low to be detected by standard laboratory techniques. No influence on colonization rates was found for sex, race, season, economic status, or common childhood infectious diseases such as coryza or otitis media."}
{"id": "PMID:1086856", "title": "Pulmonary abnormalities in intermediate alpha-1-antitrypsin deficiency.", "content": "Pulmonary function studies were carried out in a group of asymptomatic nonsmoking adults with intermediate alpha-1-antitrypsin deficiency who were attending an early disease detection unit in Rochester, N. Y. All subjects were identified by specific protease inhibitor (Pi) typing. Fifteen MZ and 14 MS subjects who had never smoked cigarettes were matched by sex and age to MM controls. Spirometry, static lung volumes, and single breath-diffusing capacity were identical in all Pi type groups with no statistically significant differences noted. Maximal expiratory flow volume curves were obtained in all subjects. MZ subjects demonstrated statistically impaired maximal flow rates at 75%, 50%, and 25% of vital capacity compared to their MM controls. Total pulmonary resistance by the oscillometric method was measured at 3, 5, 7, and 9 cycle/s in the same subjects. Increased frequency dependence of resistance (defined as the difference between total pulmonary resistance at 3 cycle/s and 9 cycle/s) was observed in MZ subjects compared to MM controls. No differences were noted by this method in MS-MM pairs. The data suggest that detectable mechanical abnormalities are present in subjects with the MZ phenotype, even in the absence of established risk factors such as cigarette smoking and high air pollution.", "contents": "Pulmonary abnormalities in intermediate alpha-1-antitrypsin deficiency. Pulmonary function studies were carried out in a group of asymptomatic nonsmoking adults with intermediate alpha-1-antitrypsin deficiency who were attending an early disease detection unit in Rochester, N. Y. All subjects were identified by specific protease inhibitor (Pi) typing. Fifteen MZ and 14 MS subjects who had never smoked cigarettes were matched by sex and age to MM controls. Spirometry, static lung volumes, and single breath-diffusing capacity were identical in all Pi type groups with no statistically significant differences noted. Maximal expiratory flow volume curves were obtained in all subjects. MZ subjects demonstrated statistically impaired maximal flow rates at 75%, 50%, and 25% of vital capacity compared to their MM controls. Total pulmonary resistance by the oscillometric method was measured at 3, 5, 7, and 9 cycle/s in the same subjects. Increased frequency dependence of resistance (defined as the difference between total pulmonary resistance at 3 cycle/s and 9 cycle/s) was observed in MZ subjects compared to MM controls. No differences were noted by this method in MS-MM pairs. The data suggest that detectable mechanical abnormalities are present in subjects with the MZ phenotype, even in the absence of established risk factors such as cigarette smoking and high air pollution."}
{"id": "PMID:1086857", "title": "Radioimmunoassay of the binding protein for vitamin D and its metabolites in human serum: concentrations in normal subjects and patients with disorders of mineral homeostasis.", "content": "A radioimmunoassay for the binding protein for vitamin D and its metabolites (DBP) has been developed. Suitable rabbit anti-DBP antiserum was elicited after primary and one booster injection. Anti-DBP antisera, as well as antigroup-specific component antisera, produced a single, monospecific line of percipitation when reacted against purified DBP and human serum. DBP was iodinated with 125I and 125I-DBP was purified by gel filtration on Sephadex G-200. Binding of 125I-DBP by 20 nl of rabbit anti-DBP antisera was approximately 50% and was sharply competed for by 0.4-4.0 ng of DBP standard. Displacement of 125I-DBP by human serum dilutions or standard DBP gave identical curves, and only weak competition was observed with old and new world primate sera. Apo- and holo-DBP possessed indistinguishable immunoreactivity. The assay detects DBP in 1-10 nl of human serum with reasonable accuracy and with reasonable intra- and interassay precision. The mean serum concentration (+/- SEM) for a group of 40 normal adults was 525 +/- 24 mug/ml and no sex difference was observed. Higher levels were found in sera from pregnant women and women receiving oral contraceptives, and decreased concentrations were observed in premature cord and hypoproteinemic sera. No significant correlation between serum DBP levels and serum 25-hydroxycalciferol levels was found, and the DBP content of sera from vitamin D-deprived and vitamin D-treated subjects was indistinguishable from that of normal adults. DBP accounts for 6- of the alpha globulin in normal human serum. Considering the normal serum content of the parent vitamin and its metabolites to be approximately 0.1-0.2 mum, these immunoassay data confirm previous saturation analyses of human serum antiricketic sterol-binding capacity and suggest that greater than 95% of DBP circulates as the apoprotein under normal conditions.", "contents": "Radioimmunoassay of the binding protein for vitamin D and its metabolites in human serum: concentrations in normal subjects and patients with disorders of mineral homeostasis. A radioimmunoassay for the binding protein for vitamin D and its metabolites (DBP) has been developed. Suitable rabbit anti-DBP antiserum was elicited after primary and one booster injection. Anti-DBP antisera, as well as antigroup-specific component antisera, produced a single, monospecific line of percipitation when reacted against purified DBP and human serum. DBP was iodinated with 125I and 125I-DBP was purified by gel filtration on Sephadex G-200. Binding of 125I-DBP by 20 nl of rabbit anti-DBP antisera was approximately 50% and was sharply competed for by 0.4-4.0 ng of DBP standard. Displacement of 125I-DBP by human serum dilutions or standard DBP gave identical curves, and only weak competition was observed with old and new world primate sera. Apo- and holo-DBP possessed indistinguishable immunoreactivity. The assay detects DBP in 1-10 nl of human serum with reasonable accuracy and with reasonable intra- and interassay precision. The mean serum concentration (+/- SEM) for a group of 40 normal adults was 525 +/- 24 mug/ml and no sex difference was observed. Higher levels were found in sera from pregnant women and women receiving oral contraceptives, and decreased concentrations were observed in premature cord and hypoproteinemic sera. No significant correlation between serum DBP levels and serum 25-hydroxycalciferol levels was found, and the DBP content of sera from vitamin D-deprived and vitamin D-treated subjects was indistinguishable from that of normal adults. DBP accounts for 6- of the alpha globulin in normal human serum. Considering the normal serum content of the parent vitamin and its metabolites to be approximately 0.1-0.2 mum, these immunoassay data confirm previous saturation analyses of human serum antiricketic sterol-binding capacity and suggest that greater than 95% of DBP circulates as the apoprotein under normal conditions."}
{"id": "PMID:1086858", "title": "Videometric analysis of regional left ventricular function before and after aortocoronary artery bypass surgery: correlation of peak rate of myocardial wall thickening with late postoperative graft flows.", "content": "The peak rate of systolic wall thickening (pdTw/dt) in regions of the left ventricle was determined by biplane roentgen videometry in 60 patients before and a median of 14 mo after aorto-coronary bypass graft surgery. The left ventricular ejection fraction, stroke volume, and end-diastolic volume and pressure did not change significantly after surgery in the presence of patent or occluded grafts (P greater than 0.05). Statistically significant increases occurred in the peak rate of systolic wall thickening regions supplied by patent bypass grafts, and significant decreases occurred in regions with occluded grafts (P less than 0.01). Of 42 preoperatively hypokinetic regions (pdTw/dt greater than 0 less than 5.0 cm/s) supplied by a patent graft, 30 improved by an average of 2.6 cm/s after operation; 18 returned to normal. Failure of 24 hypokinetic regions to improve to normal was associated with myocardial infarction in 11 or with late postoperative graft blood flows of less than 60 ml/min measured by videodensitometry, in 10. All seven preoperatively akinetic (pdTw/dt=0) or dyskinetic (pdTw/dt less than 0) regions did not improve after the operation despite the fact that, in five of the seven, coronary bypass flows were over 60 ml/min. All eight preoperatively hypokinetic regions supplied by coronary artery graft flows of less than or equal 40 ml/min failed to improve to normal after operation. All nine preoperatively hypokinetic regions supplied by coronary artery graft flows of over 60 ml/min improved to normal after surgery. Late postoperative coronary artery bypass graft flows, the functional status of the myocardium, the status and distribution of the native coronary circulation, and decreased regional function elsewhere in the ventricle must all be considered when regional left ventricular function is interpreted.", "contents": "Videometric analysis of regional left ventricular function before and after aortocoronary artery bypass surgery: correlation of peak rate of myocardial wall thickening with late postoperative graft flows. The peak rate of systolic wall thickening (pdTw/dt) in regions of the left ventricle was determined by biplane roentgen videometry in 60 patients before and a median of 14 mo after aorto-coronary bypass graft surgery. The left ventricular ejection fraction, stroke volume, and end-diastolic volume and pressure did not change significantly after surgery in the presence of patent or occluded grafts (P greater than 0.05). Statistically significant increases occurred in the peak rate of systolic wall thickening regions supplied by patent bypass grafts, and significant decreases occurred in regions with occluded grafts (P less than 0.01). Of 42 preoperatively hypokinetic regions (pdTw/dt greater than 0 less than 5.0 cm/s) supplied by a patent graft, 30 improved by an average of 2.6 cm/s after operation; 18 returned to normal. Failure of 24 hypokinetic regions to improve to normal was associated with myocardial infarction in 11 or with late postoperative graft blood flows of less than 60 ml/min measured by videodensitometry, in 10. All seven preoperatively akinetic (pdTw/dt=0) or dyskinetic (pdTw/dt less than 0) regions did not improve after the operation despite the fact that, in five of the seven, coronary bypass flows were over 60 ml/min. All eight preoperatively hypokinetic regions supplied by coronary artery graft flows of less than or equal 40 ml/min failed to improve to normal after operation. All nine preoperatively hypokinetic regions supplied by coronary artery graft flows of over 60 ml/min improved to normal after surgery. Late postoperative coronary artery bypass graft flows, the functional status of the myocardium, the status and distribution of the native coronary circulation, and decreased regional function elsewhere in the ventricle must all be considered when regional left ventricular function is interpreted."}
{"id": "PMID:1086859", "title": "Milk fever in dairy cows. VII. Effect of continuous vitamin D feeding on incidence of milk fever.", "content": "Feeding of 20 to 30 million units of vitamin D for 3 to 8 days prepartum previously prevented about 80% of expected milk fever cases in mature Jersey cows with histories of milk fever. In this experiment vitamin D was fed continuously through the year via 32,000 units of vitamin D added to each .455 kg of concentrate fed (approximately 100,000 to 580,000 units/day). Milk fever incidence was measured after 5 yr of age (third calf) at 43 parturitions in mature Jersey cows with histories of milk fever and at 139 parturitions in mature Jersey cows with no previous milk fever. Continuous feeding of vitamin D reduced incidence of milk fever in cows with previous milk fever from 60% in the controls to 26.1% in the group fed vitamin D. In the cows with no milk fever previously, feeding vitamin D did not reduce incidence of milk fever (controls 23.7%, vitamin-D-fed 28.3%).", "contents": "Milk fever in dairy cows. VII. Effect of continuous vitamin D feeding on incidence of milk fever. Feeding of 20 to 30 million units of vitamin D for 3 to 8 days prepartum previously prevented about 80% of expected milk fever cases in mature Jersey cows with histories of milk fever. In this experiment vitamin D was fed continuously through the year via 32,000 units of vitamin D added to each .455 kg of concentrate fed (approximately 100,000 to 580,000 units/day). Milk fever incidence was measured after 5 yr of age (third calf) at 43 parturitions in mature Jersey cows with histories of milk fever and at 139 parturitions in mature Jersey cows with no previous milk fever. Continuous feeding of vitamin D reduced incidence of milk fever in cows with previous milk fever from 60% in the controls to 26.1% in the group fed vitamin D. In the cows with no milk fever previously, feeding vitamin D did not reduce incidence of milk fever (controls 23.7%, vitamin-D-fed 28.3%)."}
{"id": "PMID:1086860", "title": "Massive postoperative hemorrhage from hepatic artery erosion.", "content": "A 66-year-old male patient who had undergone repeated operations for peptic ulcer disease involving the right upper abdominal quadrant, developed cholecystitis with calculous obstruction of the common bile duct. The gallbladder was removed. Later, an operation was performed for removal of a residual stone from the common duct. At this time an anomalous arterial structure was noted about the duct. Hemorrhage occurred ten days postoperatively, and the anomalous hepatic artery was found to be eroded. The bleeding was controlled. During the succeeding two weeks there were four episodes of bleeding (involving erosion of the hepatic artery and adjacent tissues), three of which were controlled. The fourth episode ended in the death of the patient from exsanguination secondary to bleeding from stress ulcers in the gastric remnant. At no time did the laboratory data unequivocally indicate an abnormality of blood coagulation. Erosion of the anomalous cystic artery apparently precipitated the fatal chain of events.", "contents": "Massive postoperative hemorrhage from hepatic artery erosion. A 66-year-old male patient who had undergone repeated operations for peptic ulcer disease involving the right upper abdominal quadrant, developed cholecystitis with calculous obstruction of the common bile duct. The gallbladder was removed. Later, an operation was performed for removal of a residual stone from the common duct. At this time an anomalous arterial structure was noted about the duct. Hemorrhage occurred ten days postoperatively, and the anomalous hepatic artery was found to be eroded. The bleeding was controlled. During the succeeding two weeks there were four episodes of bleeding (involving erosion of the hepatic artery and adjacent tissues), three of which were controlled. The fourth episode ended in the death of the patient from exsanguination secondary to bleeding from stress ulcers in the gastric remnant. At no time did the laboratory data unequivocally indicate an abnormality of blood coagulation. Erosion of the anomalous cystic artery apparently precipitated the fatal chain of events."}
{"id": "PMID:1086861", "title": "Evidence for separate subpopulations of B cells responding to T-independent and T-dependent immunogens.", "content": "BALB/c spleen cell cultures were double-challenged with optimal doses of TNP-T independent and TNP-T-dependent immunogens. The anti-hapten response was additive or synergistic in double challenged cultures compared to the response in cultures challenged with either immunogen alone. A high, suppressive dose of either immunogen did not prevent the response to an optimal dose of the second immunogen in the same culture. Spleen cell cultures were also double challenged with combinations of two T-dependent or two T-independent immunogens. Addition or synergy in the anti-hapten response only occurred when the double challenge consisted of a mixutre of T-dependent and T-independent immunogens. These results are consistent with the proposed B1B2 classification of B cell subpopulations.", "contents": "Evidence for separate subpopulations of B cells responding to T-independent and T-dependent immunogens. BALB/c spleen cell cultures were double-challenged with optimal doses of TNP-T independent and TNP-T-dependent immunogens. The anti-hapten response was additive or synergistic in double challenged cultures compared to the response in cultures challenged with either immunogen alone. A high, suppressive dose of either immunogen did not prevent the response to an optimal dose of the second immunogen in the same culture. Spleen cell cultures were also double challenged with combinations of two T-dependent or two T-independent immunogens. Addition or synergy in the anti-hapten response only occurred when the double challenge consisted of a mixutre of T-dependent and T-independent immunogens. These results are consistent with the proposed B1B2 classification of B cell subpopulations."}
{"id": "PMID:1086862", "title": "Membrane expression of Thy-1,2 and GM1 ganglioside on differentiating T lymphocytes.", "content": "C3H mouse bone marrow cells were separated by discontinuous BSA gradient centrifugation. Marrow cells from the 17 to 19%, 19 to 21% to 23%, 23 to 25%, 25 to 27% interfaces and the cell pellet were treated with thymic factor (TF) or with Vibrio cholerae neuraminidase (VCN), followed by anti-Thy-1,2 and anti-GM1 ganglioside antisera. Antigens determined by anti-Thy-1.2 or anti-GM1 were expressed either with TF or VCN within a 30-min incubation. Cells expressing these antigens after VCN or TF treatment were concentrated in the 19 to 21% layer and the 21 to 23% layer whereas there was little or no antiserum cytotoxicity in the other layers. A small amount of Thy-1.2 of GM1 could be detected by cytotoxicity on 19 to 21% layer marrow cells within 15 min of either TF or VCN treatment. Treatment of 19 to 21% layer cells with TF or VCN had no effect on anti-H-2 cytotoxicity. Pretreatment of TF-treated marrow cells with cholera toxin or choleragenoid (which bind cell surface GM1) abrogated the cytotoxicity of anti-Thy-1.2 or anti-GM1 without affecting anti-H-2 cytotoxicity. Pretreatment of Thy-1.2 positive C3H thymocytes with cholera toxin or choleragenoid greatly reduced the cytotoxicity of anti-Thy-1.2 antisera without affecting the cytotoxicity of anti-H-2 antisera. Nude mouse splenocytes, after treatment with VCN or TF, were susceptible to the lytic action of anti-Thy-1.2 and anti-GM1. The possibility that nonspecific autologous antibodies were responsible for anti-Thy-1.2 cytotoxicity toward VCN-treated marrow cells was eliminated because anti-Thy-1.2 was not cytotoxic for VCN treated AKR (Thy-1.1) marrow cells.", "contents": "Membrane expression of Thy-1,2 and GM1 ganglioside on differentiating T lymphocytes. C3H mouse bone marrow cells were separated by discontinuous BSA gradient centrifugation. Marrow cells from the 17 to 19%, 19 to 21% to 23%, 23 to 25%, 25 to 27% interfaces and the cell pellet were treated with thymic factor (TF) or with Vibrio cholerae neuraminidase (VCN), followed by anti-Thy-1,2 and anti-GM1 ganglioside antisera. Antigens determined by anti-Thy-1.2 or anti-GM1 were expressed either with TF or VCN within a 30-min incubation. Cells expressing these antigens after VCN or TF treatment were concentrated in the 19 to 21% layer and the 21 to 23% layer whereas there was little or no antiserum cytotoxicity in the other layers. A small amount of Thy-1.2 of GM1 could be detected by cytotoxicity on 19 to 21% layer marrow cells within 15 min of either TF or VCN treatment. Treatment of 19 to 21% layer cells with TF or VCN had no effect on anti-H-2 cytotoxicity. Pretreatment of TF-treated marrow cells with cholera toxin or choleragenoid (which bind cell surface GM1) abrogated the cytotoxicity of anti-Thy-1.2 or anti-GM1 without affecting anti-H-2 cytotoxicity. Pretreatment of Thy-1.2 positive C3H thymocytes with cholera toxin or choleragenoid greatly reduced the cytotoxicity of anti-Thy-1.2 antisera without affecting the cytotoxicity of anti-H-2 antisera. Nude mouse splenocytes, after treatment with VCN or TF, were susceptible to the lytic action of anti-Thy-1.2 and anti-GM1. The possibility that nonspecific autologous antibodies were responsible for anti-Thy-1.2 cytotoxicity toward VCN-treated marrow cells was eliminated because anti-Thy-1.2 was not cytotoxic for VCN treated AKR (Thy-1.1) marrow cells."}
{"id": "PMID:1086863", "title": "Studies of surface immunoglobulins on human B lymphocytes. II. Characterization of a population of lymphocytes lacking surface immunoglobulins but carrying Fc receptor (SIg-Fc+ cell).", "content": "Surface markers and organ distributions of B lymphocytes bearing surface immunoglobulins (SIg B) and lymphocytes lacking SIg but carrying Fc receptors (SIg-Fc+), and the variations of both the cell populations in the autoimmune diseases were studied. Peripheral blood lymphocytes contained approximately 9% SIg B and 22% SIg-Fc+ cells. Both the populations of SIg B and SIg-Fc+ cells were included in the cells that formed no E-rosettes. When previously treated with neuraminidase, however, the SIg-Fc+ cells formed E-rosettes, whereas SIg B showed no ability to form rosettes. C receptors as detected by human complement were found in the large portions of SIg B but in a minority of the SIg-Fc+ cell population. The tissues that contained the highest concentrations of SIg B and SIg-Fc+ cells were the spleen and peripheral blood, respectively. In the patients with an autoimmune disease in active stages SIg B cells in the peripheral blood markedly increased and, reversed, SIg-Fc+ cells decreased; in contrast, in remission of the diseases SIg B cells dropped down and SIg-Fc+ increased up to their normal levels. A possible relationship between the SIg B and SIg-Fc+ cells in regard to the differentiation of B lymphocytes was presemed.", "contents": "Studies of surface immunoglobulins on human B lymphocytes. II. Characterization of a population of lymphocytes lacking surface immunoglobulins but carrying Fc receptor (SIg-Fc+ cell). Surface markers and organ distributions of B lymphocytes bearing surface immunoglobulins (SIg B) and lymphocytes lacking SIg but carrying Fc receptors (SIg-Fc+), and the variations of both the cell populations in the autoimmune diseases were studied. Peripheral blood lymphocytes contained approximately 9% SIg B and 22% SIg-Fc+ cells. Both the populations of SIg B and SIg-Fc+ cells were included in the cells that formed no E-rosettes. When previously treated with neuraminidase, however, the SIg-Fc+ cells formed E-rosettes, whereas SIg B showed no ability to form rosettes. C receptors as detected by human complement were found in the large portions of SIg B but in a minority of the SIg-Fc+ cell population. The tissues that contained the highest concentrations of SIg B and SIg-Fc+ cells were the spleen and peripheral blood, respectively. In the patients with an autoimmune disease in active stages SIg B cells in the peripheral blood markedly increased and, reversed, SIg-Fc+ cells decreased; in contrast, in remission of the diseases SIg B cells dropped down and SIg-Fc+ increased up to their normal levels. A possible relationship between the SIg B and SIg-Fc+ cells in regard to the differentiation of B lymphocytes was presemed."}
{"id": "PMID:1086864", "title": "Complement receptor lymphocytes in the rabbit. II. The effect of immunization on the complement receptor lymphocyte population in the craining lymph node.", "content": "In an attempt to determine the effect of immunization on the size and nature of the complement receptor lymphocyte (CRL) and surface immunoglobulin (SIg)-bearing lymphocyte (B cell) populations, rabbits were injected with 1 mg alum-precipitated keyhole limpet hemocyanin and the cells of the draining popliteal lymph nodes were examined at various intervals for these populations. Comparison to unimmunized control lymph nodes revealed that immunization resulted in an approximate doubling of the percentages of both CRL and B cell populations by the peak of the response, while the cell number increased about 7-to 8-fold. Kinetic analysis of the response indicated that the percentages of CRL and B cells increased essentially in parallel beginning within 40 h after immunization. Simultaneous assay of complement receptor (CR) and SIg disclosed that CRL remained a B cell subpopulation throughout the response. To determine whether cell division could account for the increases in total cell number and per cent CRL and B cells, immunized rabbits were given 3H-thymidine in vivo, and the fraction of labeled (dividing) cells was determined by autoradiography. Results of such experiments were consistent with the possibility that the early increase in cell number and the increase in CRL and B cell percentages were a result of trapping and/or homing of lymphocytes migrating from the peripheral blood. A significant amount of lymphocyte division occurred during later stages of the response.", "contents": "Complement receptor lymphocytes in the rabbit. II. The effect of immunization on the complement receptor lymphocyte population in the craining lymph node. In an attempt to determine the effect of immunization on the size and nature of the complement receptor lymphocyte (CRL) and surface immunoglobulin (SIg)-bearing lymphocyte (B cell) populations, rabbits were injected with 1 mg alum-precipitated keyhole limpet hemocyanin and the cells of the draining popliteal lymph nodes were examined at various intervals for these populations. Comparison to unimmunized control lymph nodes revealed that immunization resulted in an approximate doubling of the percentages of both CRL and B cell populations by the peak of the response, while the cell number increased about 7-to 8-fold. Kinetic analysis of the response indicated that the percentages of CRL and B cells increased essentially in parallel beginning within 40 h after immunization. Simultaneous assay of complement receptor (CR) and SIg disclosed that CRL remained a B cell subpopulation throughout the response. To determine whether cell division could account for the increases in total cell number and per cent CRL and B cells, immunized rabbits were given 3H-thymidine in vivo, and the fraction of labeled (dividing) cells was determined by autoradiography. Results of such experiments were consistent with the possibility that the early increase in cell number and the increase in CRL and B cell percentages were a result of trapping and/or homing of lymphocytes migrating from the peripheral blood. A significant amount of lymphocyte division occurred during later stages of the response."}
{"id": "PMID:1086865", "title": "Induction or suppression of B cell proliferation and differentiation by phytohemagglutinin or concanavalin A in mouse spleen cell cultures.", "content": "Cultures of mouse spleen cells or various mixtures of mouse T and B cells were stimulated with PHA or Con A, and the T, B, or plasmablast nature of the transformed cells, was determined by immunofluorescence 2 to 4 days later. The lectins enhanced B cell proliferation and plasmablast differentiation (\"helper\" effect) provided one of the following conditions was fultilled: a) suboptimal doses of lectin were used, b) cultures were performed at low cell concentration, c) cultures were made of spleen cells containing a small percentage of T cells, d) the cultures contained a mixture of T-depleted spleen cells and T cells rendered unable to proliferate by irradiation. In contrast, cultures performed with 1.5 10(6) or more spleen cells/ml and optimal doses of lectin contained almost exclusively T blasts, as did cultures stimulated in the same conditions with both PHA and LPS. This last observation idicates the existence of a lectin-induced \"suppressor\" effect, since LPS, a B cell mitogen, induces, in the absence of PHA, a marked B cell proliferation and differentiation into plasmablasts. These helper and suppressor effects were entirely mediated by T cells, since they were not observed in spleen cell cultures depleted in T cells by anti-thets + C. Analysis of the cultures by immunofluorescence and radioautography after pulses of 3H-thymidine showed that these antagonistic effects could be related to the number of T blasts present in the culture and to their proliferative behavior. Heoper effect is observed in cultures containing a relatively low number of T blasts (or none in cultures made with irradiated T cells), whereas suppressive effect is observed in cultures containing a high number of T blasts, a large proportion of them having left the proliferative cell cycle. It is proposed that when a critical concentration of T blasts is reached (\"saturation density\"), further proliferation and differentiation is prevented, resulting in a suppressive effect on the generation of plasmablasts. The helper effect of lectin-activated T cells seems to be exerted on a subpopulation of B cells which was, at least in part, already proliferating in vivo, and to result in a polyclonal IgM plasmablast differentiation.", "contents": "Induction or suppression of B cell proliferation and differentiation by phytohemagglutinin or concanavalin A in mouse spleen cell cultures. Cultures of mouse spleen cells or various mixtures of mouse T and B cells were stimulated with PHA or Con A, and the T, B, or plasmablast nature of the transformed cells, was determined by immunofluorescence 2 to 4 days later. The lectins enhanced B cell proliferation and plasmablast differentiation (\"helper\" effect) provided one of the following conditions was fultilled: a) suboptimal doses of lectin were used, b) cultures were performed at low cell concentration, c) cultures were made of spleen cells containing a small percentage of T cells, d) the cultures contained a mixture of T-depleted spleen cells and T cells rendered unable to proliferate by irradiation. In contrast, cultures performed with 1.5 10(6) or more spleen cells/ml and optimal doses of lectin contained almost exclusively T blasts, as did cultures stimulated in the same conditions with both PHA and LPS. This last observation idicates the existence of a lectin-induced \"suppressor\" effect, since LPS, a B cell mitogen, induces, in the absence of PHA, a marked B cell proliferation and differentiation into plasmablasts. These helper and suppressor effects were entirely mediated by T cells, since they were not observed in spleen cell cultures depleted in T cells by anti-thets + C. Analysis of the cultures by immunofluorescence and radioautography after pulses of 3H-thymidine showed that these antagonistic effects could be related to the number of T blasts present in the culture and to their proliferative behavior. Heoper effect is observed in cultures containing a relatively low number of T blasts (or none in cultures made with irradiated T cells), whereas suppressive effect is observed in cultures containing a high number of T blasts, a large proportion of them having left the proliferative cell cycle. It is proposed that when a critical concentration of T blasts is reached (\"saturation density\"), further proliferation and differentiation is prevented, resulting in a suppressive effect on the generation of plasmablasts. The helper effect of lectin-activated T cells seems to be exerted on a subpopulation of B cells which was, at least in part, already proliferating in vivo, and to result in a polyclonal IgM plasmablast differentiation."}
{"id": "PMID:1086866", "title": "Role of T lymphocytes in adjuvant arthritis. I. Evidence for the regulatory function of thymus-derived cells in the induction of the disease.", "content": "Rats of Wistar King Aptekman (WKA) were thymectomized at 4 weeks of age and injected with Mycobacterium tuberculosis wax D 6 weeks after the operation to induce adjuvant arthritis. The development of this disease was strikingly enhanced by this treatment. Further experiments showed that a 4- to 6-week interval between thymectomy and wax D injection was necessary to show the enhancing effect. Such enhancement by thymectomy was also shown in rats of another strain, Sprague-Dawley (SD). The enhancing effect of thymectomy was abolished when thymocytes of normal syngeneic rats were transferred to thymectomized rats 7 days before the wax D inoculation. Furthermore, severe arthritis was also produced in WKA rats that were pretreated with low dose (200 R)  whole body irradiation, but not in those treated with higher doses (400 to 700 R). These results seem to indicate that the enhancing effect is brought about by selective depletion of a certain population of T lymphocytes. The population depleted may be thymus dependent, short-lived and radiosensitive, the properties of which agree whith those known for suppressor T lymphocytes. Thus, it appears that thymus-derived cells could normally exert a regulatory effect on the development of adjuvant arthritis which might render these rat strains relatively less susceptible to this disease.", "contents": "Role of T lymphocytes in adjuvant arthritis. I. Evidence for the regulatory function of thymus-derived cells in the induction of the disease. Rats of Wistar King Aptekman (WKA) were thymectomized at 4 weeks of age and injected with Mycobacterium tuberculosis wax D 6 weeks after the operation to induce adjuvant arthritis. The development of this disease was strikingly enhanced by this treatment. Further experiments showed that a 4- to 6-week interval between thymectomy and wax D injection was necessary to show the enhancing effect. Such enhancement by thymectomy was also shown in rats of another strain, Sprague-Dawley (SD). The enhancing effect of thymectomy was abolished when thymocytes of normal syngeneic rats were transferred to thymectomized rats 7 days before the wax D inoculation. Furthermore, severe arthritis was also produced in WKA rats that were pretreated with low dose (200 R)  whole body irradiation, but not in those treated with higher doses (400 to 700 R). These results seem to indicate that the enhancing effect is brought about by selective depletion of a certain population of T lymphocytes. The population depleted may be thymus dependent, short-lived and radiosensitive, the properties of which agree whith those known for suppressor T lymphocytes. Thus, it appears that thymus-derived cells could normally exert a regulatory effect on the development of adjuvant arthritis which might render these rat strains relatively less susceptible to this disease."}
{"id": "PMID:1086867", "title": "Electrophoretic separation of radioisotopically labeled mouse lymphocytes.", "content": "A new preparative method is described for the separation and direct electrophoretic comparison of the distribution profile of radioisotopically labeled cells. The cell populations to be compared are labeled separately with different radioisotopes (e.g., 51Cr and 99mTc) and mixed. The cell mixture is subjected to density gradient electrophoresis and the distribution of each radioisotope in the collected fractions is ascertained by differential gamma counting. In the case of uniform label uptake by the cells in each distribution, the radioactivity counts represent cell frequency. Nonuniform labeling allows useful comparisons only in terms of relative mobility distribution. Model experiments performed with 51Cr- and 99mTc-labeled mouse thymocytes and spleen cells are in general agreement with mobility distribution results obtained previously by free flow electrophoresis.", "contents": "Electrophoretic separation of radioisotopically labeled mouse lymphocytes. A new preparative method is described for the separation and direct electrophoretic comparison of the distribution profile of radioisotopically labeled cells. The cell populations to be compared are labeled separately with different radioisotopes (e.g., 51Cr and 99mTc) and mixed. The cell mixture is subjected to density gradient electrophoresis and the distribution of each radioisotope in the collected fractions is ascertained by differential gamma counting. In the case of uniform label uptake by the cells in each distribution, the radioactivity counts represent cell frequency. Nonuniform labeling allows useful comparisons only in terms of relative mobility distribution. Model experiments performed with 51Cr- and 99mTc-labeled mouse thymocytes and spleen cells are in general agreement with mobility distribution results obtained previously by free flow electrophoresis."}
{"id": "PMID:1086868", "title": "Genetic control of immune responsiveness to poly (LPro)--poly (LLys)-derived polypeptides by histocompatibility-linked immune response genes in the rat.", "content": "In rats responsiveness to branched synthetic polypeptides carrying a Pro--L backbone, such as (T,G)-Pro--L or (Phe,G)-Pro-L and to Pro--L itself is controlled by Ir genes which are linked to the major histocompatibility genes. The level of antibody production to these polypeptides does not fall into strict high or low responder categories but covers the range in between. (T,G)-pro--L and Pro--L elicit a very similar response pattern which, however, differs from that obtained with (Phe,G)-Pro--L. Anti-(T,G),Pro--L antibodies do not cross-react with (T,G)-A--L, but do so extensively with Pro--L. Anti-(Phe,G)-Pro--L antibodies show cross-reactivity to (Phe,G)-A--L only when the antibody-producing strain is a high responder to (Phe,G)-A--L. These results when considered in view of data obtained in mice on genetic control of the immune response to (T,G)-Pro--L suggest that at least two unlinked Ir genes are involved in controlling anti-Pro--L responsiveness.", "contents": "Genetic control of immune responsiveness to poly (LPro)--poly (LLys)-derived polypeptides by histocompatibility-linked immune response genes in the rat. In rats responsiveness to branched synthetic polypeptides carrying a Pro--L backbone, such as (T,G)-Pro--L or (Phe,G)-Pro-L and to Pro--L itself is controlled by Ir genes which are linked to the major histocompatibility genes. The level of antibody production to these polypeptides does not fall into strict high or low responder categories but covers the range in between. (T,G)-pro--L and Pro--L elicit a very similar response pattern which, however, differs from that obtained with (Phe,G)-Pro--L. Anti-(T,G),Pro--L antibodies do not cross-react with (T,G)-A--L, but do so extensively with Pro--L. Anti-(Phe,G)-Pro--L antibodies show cross-reactivity to (Phe,G)-A--L only when the antibody-producing strain is a high responder to (Phe,G)-A--L. These results when considered in view of data obtained in mice on genetic control of the immune response to (T,G)-Pro--L suggest that at least two unlinked Ir genes are involved in controlling anti-Pro--L responsiveness."}
{"id": "PMID:1086869", "title": "Cytotoxic macrophages: a rapid nonspecific response to viral infection.", "content": "In vitro and in vivo assays have been developed to study the relative contributions of various types of immune cytolysis in the destruction of infected cells after Semliki Forest virus infection of BALB/c mice. Highly cytotoxic activated macrophages, not specific for the infecting virus, appear on day 1, peak on day 2 to 3, and disappear within a week. Specifically sensitized T cells appear around day 3, peak on day 6, and disappear within a month. Cytotoxic antibody appears on day 4 and reaches high titers by day 8. Immune spleen cells greatly reduce the yield of virus from cultured cells. Infected cells rapidly disappear after transfer to infected animals.", "contents": "Cytotoxic macrophages: a rapid nonspecific response to viral infection. In vitro and in vivo assays have been developed to study the relative contributions of various types of immune cytolysis in the destruction of infected cells after Semliki Forest virus infection of BALB/c mice. Highly cytotoxic activated macrophages, not specific for the infecting virus, appear on day 1, peak on day 2 to 3, and disappear within a week. Specifically sensitized T cells appear around day 3, peak on day 6, and disappear within a month. Cytotoxic antibody appears on day 4 and reaches high titers by day 8. Immune spleen cells greatly reduce the yield of virus from cultured cells. Infected cells rapidly disappear after transfer to infected animals."}
{"id": "PMID:1086870", "title": "Functional heterongeneity among the T-derived lymphocytes of the mouse. VI. Memory T cells stored in the T2 subpopulation.", "content": "Memory T cells were demonstrated in mice which were the precursors for cells causing sheep red blood cell-(SRBC) specific helper activity in the in vitro response of spleen cells to trinitrophenyl (TNP)-SRBC. These memory cells were distinguished from the effectors of helper activity generated during the primary response and were shown to belong to the T2 subpopulation of peripheral T cells on the basis of 1) their rapid response to a challenge with SRBC, 2) their long lifetime, and 3) their sensitivity to small in vivo doses of anti-thymocyte serum.", "contents": "Functional heterongeneity among the T-derived lymphocytes of the mouse. VI. Memory T cells stored in the T2 subpopulation. Memory T cells were demonstrated in mice which were the precursors for cells causing sheep red blood cell-(SRBC) specific helper activity in the in vitro response of spleen cells to trinitrophenyl (TNP)-SRBC. These memory cells were distinguished from the effectors of helper activity generated during the primary response and were shown to belong to the T2 subpopulation of peripheral T cells on the basis of 1) their rapid response to a challenge with SRBC, 2) their long lifetime, and 3) their sensitivity to small in vivo doses of anti-thymocyte serum."}
{"id": "PMID:1086871", "title": "Mouse B and T lymphocyte responses to purified timothy pollen antigens in vitro.", "content": "Purified populations of splenic B and T lymphocytes from LAF mice immunized with a crude extract of timothy pollen (WST) responded specifically to pollen antigens in an in vitro lymphocyte transformation system. The peak lymphocyte transformation response occurred 5 days after a secondary immunization and was the result of T-B cell cooperation in vitro. Wtih two purified pollen antigens as in vitro stimulants we were able to define at least two antigen-specific population of B cells and one population of T cells. These results were confirmed by inhibition studies with a monovalent hapten from WST, Antigen D.", "contents": "Mouse B and T lymphocyte responses to purified timothy pollen antigens in vitro. Purified populations of splenic B and T lymphocytes from LAF mice immunized with a crude extract of timothy pollen (WST) responded specifically to pollen antigens in an in vitro lymphocyte transformation system. The peak lymphocyte transformation response occurred 5 days after a secondary immunization and was the result of T-B cell cooperation in vitro. Wtih two purified pollen antigens as in vitro stimulants we were able to define at least two antigen-specific population of B cells and one population of T cells. These results were confirmed by inhibition studies with a monovalent hapten from WST, Antigen D."}
{"id": "PMID:1086872", "title": "Subpopulations of chicken B lymphocytes.", "content": "Immunoglobulin-synthesizing cells from the spleen and bursa were fractionated by the 1 X G sedimentation velocity technique and characterized by their ability to synthesize immunoglobulin and by staining with fluorescent anti-light chain chain. Four subpopulations of immunoglobulin-synthesizing cells were identified. In the bursa, slowly sedimenting (S 2.3 mm/hr) and rapidly sedimenting (S greater than 3.5 mm/hr) subpopulations with surface immunoglobulin were present; in the spleen, a slowly sedimenting (S 2.3 mm/hr) subpopulation with surface immunoglobulin and plasma cells (S greater than 3.5 mm/hr) with large concentrations of intracellular immunoglobulin existed. The subpopulations differed most markedly in their ability to synthesize immunoglobulin (cpm Ig synthesized/10(6) Ig-positive cells); the rates of immunoglobulin synthesis were in the ratio 1:2:1:900. The slowly sedimenting B cells from the spleen and both subpopulations of B cells from the bursa released small amounts of immunoglobulin into the culture media, whereas, the plasma cells released immunoglobulin at a rate as much as 3700 times greater. Bursal B cells could be further distinguished from splenic B cells by a greater amount of DNA synthesis.", "contents": "Subpopulations of chicken B lymphocytes. Immunoglobulin-synthesizing cells from the spleen and bursa were fractionated by the 1 X G sedimentation velocity technique and characterized by their ability to synthesize immunoglobulin and by staining with fluorescent anti-light chain chain. Four subpopulations of immunoglobulin-synthesizing cells were identified. In the bursa, slowly sedimenting (S 2.3 mm/hr) and rapidly sedimenting (S greater than 3.5 mm/hr) subpopulations with surface immunoglobulin were present; in the spleen, a slowly sedimenting (S 2.3 mm/hr) subpopulation with surface immunoglobulin and plasma cells (S greater than 3.5 mm/hr) with large concentrations of intracellular immunoglobulin existed. The subpopulations differed most markedly in their ability to synthesize immunoglobulin (cpm Ig synthesized/10(6) Ig-positive cells); the rates of immunoglobulin synthesis were in the ratio 1:2:1:900. The slowly sedimenting B cells from the spleen and both subpopulations of B cells from the bursa released small amounts of immunoglobulin into the culture media, whereas, the plasma cells released immunoglobulin at a rate as much as 3700 times greater. Bursal B cells could be further distinguished from splenic B cells by a greater amount of DNA synthesis."}
{"id": "PMID:1086873", "title": "Fractionation of cytotoxic cells from tumour-immune rats on derivatized collagen gels.", "content": "Incubation at 37 degrees C of spleen cells from rats, immunized with Gross virus-induced lymphoma, on collagen gels coated with membrane extracts of lymphoma cells yielded two different cell populations. The non-adherent cells showed greatly diminished cytotocicity for lymphoma cells compared with unfractionated spleen cell suspensions, whilst the specifically adherent cells which were recovered after collagenase digestion of the gels, showed enrichment of cytotoxic activity.", "contents": "Fractionation of cytotoxic cells from tumour-immune rats on derivatized collagen gels. Incubation at 37 degrees C of spleen cells from rats, immunized with Gross virus-induced lymphoma, on collagen gels coated with membrane extracts of lymphoma cells yielded two different cell populations. The non-adherent cells showed greatly diminished cytotocicity for lymphoma cells compared with unfractionated spleen cell suspensions, whilst the specifically adherent cells which were recovered after collagenase digestion of the gels, showed enrichment of cytotoxic activity."}
{"id": "PMID:1086874", "title": "Studies of mouse-brain-associated T-lymphocyte antigens.", "content": "Xeno- and alloantigens shared by mouse brain, thymocytes and thymus-derived lymphocytes were isolated from brain by butanol extraction, followed by Sephadex G-200 column chromatography and preparative polyacrylamide gel electrophoresis, both in the presence of SDS. The antigenic activity, as tested by inhibition of complement-mediated cytotoxicity using a xenogeneic antiserum directed against T cell-specific antigens (anti-MTLA), was enriched 50-fold during the purification procedure. Xenogeneic and allogeneic antisera which were specific for mouse T-lymphocytes, could be obtained by immunization of rabbits and mice with the partially purified antigens.", "contents": "Studies of mouse-brain-associated T-lymphocyte antigens. Xeno- and alloantigens shared by mouse brain, thymocytes and thymus-derived lymphocytes were isolated from brain by butanol extraction, followed by Sephadex G-200 column chromatography and preparative polyacrylamide gel electrophoresis, both in the presence of SDS. The antigenic activity, as tested by inhibition of complement-mediated cytotoxicity using a xenogeneic antiserum directed against T cell-specific antigens (anti-MTLA), was enriched 50-fold during the purification procedure. Xenogeneic and allogeneic antisera which were specific for mouse T-lymphocytes, could be obtained by immunization of rabbits and mice with the partially purified antigens."}
{"id": "PMID:1086875", "title": "Serologic responses of children with meningitis due to Haemophilus influenzae type b.", "content": "The immunologic responses of 100 children hospitalized with meningitis due to Haemophilus influenzae type b were measured by the bactericidal antibody assay (BAA) and radioimmunoassay (RIA) for detection of antibody. Short-term (14-17 days after onset of illness) responses were detected by RIA alone in 20 children, by BAA alone in six, and by both tests in 23. The more sensitive RIA detected 20 children who would have been labeled \"immunologically unresponsive\" had only BAA been used. The magnitude of the antibody response was clearly related to age. Of 26 children with no immediate antibody response, 11 still had no rise in titer of antibody when restudied two to 20 months later; the remaining 15 had subsequent increases in titer. Nine of 10 children who showed an immediate antibody response remained positive when additional blood smaples were taken two to 18 months later. Over half of the children initially unresponsive to H. influenzae type b meningitis subsequently developed specific antibodies. The remainder, who failed to acquire detectable antibodies at either the acute stage of illness or late in convalescence, deserve further investigation as to the nature of their immunologic hyporesponsiveness to H. influenzae type b meningitis.", "contents": "Serologic responses of children with meningitis due to Haemophilus influenzae type b. The immunologic responses of 100 children hospitalized with meningitis due to Haemophilus influenzae type b were measured by the bactericidal antibody assay (BAA) and radioimmunoassay (RIA) for detection of antibody. Short-term (14-17 days after onset of illness) responses were detected by RIA alone in 20 children, by BAA alone in six, and by both tests in 23. The more sensitive RIA detected 20 children who would have been labeled \"immunologically unresponsive\" had only BAA been used. The magnitude of the antibody response was clearly related to age. Of 26 children with no immediate antibody response, 11 still had no rise in titer of antibody when restudied two to 20 months later; the remaining 15 had subsequent increases in titer. Nine of 10 children who showed an immediate antibody response remained positive when additional blood smaples were taken two to 18 months later. Over half of the children initially unresponsive to H. influenzae type b meningitis subsequently developed specific antibodies. The remainder, who failed to acquire detectable antibodies at either the acute stage of illness or late in convalescence, deserve further investigation as to the nature of their immunologic hyporesponsiveness to H. influenzae type b meningitis."}
{"id": "PMID:1086876", "title": "Amikacin in the treatment of gram-negative bronchopulmonary infections.", "content": "Six patients with acute gram-negative bronchopulmonary infection were treated with amikacin (15 mg/kg per day) administered intramuscularly in two equal doses at 12-hr intervals for 10-13 days. Two patients had underlying nonspecific pulmonary disease, two had advanced bronchocarcinoma, and two had extensive bronchiectasis (due to chronic aspergillosis in one patient). The pathogens were Pseudomonas aeruginosa in three patients, and Haemophilus influenzae, Klebsiella ozaenae, and Enterobacter cloacae each in one patient. Five patients recovered completely, with resolution of fever and other acute symptoms and elimination of the causative organism from sputum cultures. A moribund patient with advanced metastatic bronchocarcinoma died two days after the treatment with amikacin had been completed; the last specimen of sputum was still positive for P. aeruginosa. Tests of liver and renal function and blood counts revealed no abnormaltities. Complete audiometric survey showed no hearing loss. Nystagmography revealed reversible, lessened caloric response in some patients. Amikacin was well absorbed from the site of intramuscular injection. Levels of amikacin in serum varied among the subjects and, in some cases, for individual patients on different days.", "contents": "Amikacin in the treatment of gram-negative bronchopulmonary infections. Six patients with acute gram-negative bronchopulmonary infection were treated with amikacin (15 mg/kg per day) administered intramuscularly in two equal doses at 12-hr intervals for 10-13 days. Two patients had underlying nonspecific pulmonary disease, two had advanced bronchocarcinoma, and two had extensive bronchiectasis (due to chronic aspergillosis in one patient). The pathogens were Pseudomonas aeruginosa in three patients, and Haemophilus influenzae, Klebsiella ozaenae, and Enterobacter cloacae each in one patient. Five patients recovered completely, with resolution of fever and other acute symptoms and elimination of the causative organism from sputum cultures. A moribund patient with advanced metastatic bronchocarcinoma died two days after the treatment with amikacin had been completed; the last specimen of sputum was still positive for P. aeruginosa. Tests of liver and renal function and blood counts revealed no abnormaltities. Complete audiometric survey showed no hearing loss. Nystagmography revealed reversible, lessened caloric response in some patients. Amikacin was well absorbed from the site of intramuscular injection. Levels of amikacin in serum varied among the subjects and, in some cases, for individual patients on different days."}
{"id": "PMID:1086879", "title": "A reappraisal of cellular labeling with radiochromium salts.", "content": "The present studies demonstrate that, while erythrocytes preferentially bind hexavalent 51Cr, leukocytes bind the trivalent form with equal avidity. Red cells may be saturated with label in contrast to the binding of 51Cr to leukocytes, which may be described by first-order kinetics. Hexavalent label is rapidly reduced in leukocytes in contradistinction to the finding in red cells. The nonreutilization of label by erythrocytes and leukocytes is not due to inappropriate valency but to protein binding of the label. Unlike nonleukemic cells, leukemic leukocytes elute a large fraction of their label. These factors have been largely overlooked in many previous clinical studies using labeled cells, and they are of sufficient quantitative importance to compromise seriously the validity of numerous earlier reports.", "contents": "A reappraisal of cellular labeling with radiochromium salts. The present studies demonstrate that, while erythrocytes preferentially bind hexavalent 51Cr, leukocytes bind the trivalent form with equal avidity. Red cells may be saturated with label in contrast to the binding of 51Cr to leukocytes, which may be described by first-order kinetics. Hexavalent label is rapidly reduced in leukocytes in contradistinction to the finding in red cells. The nonreutilization of label by erythrocytes and leukocytes is not due to inappropriate valency but to protein binding of the label. Unlike nonleukemic cells, leukemic leukocytes elute a large fraction of their label. These factors have been largely overlooked in many previous clinical studies using labeled cells, and they are of sufficient quantitative importance to compromise seriously the validity of numerous earlier reports."}
{"id": "PMID:1086881", "title": "Role of B lymphocytes in cell-mediated immunity. I. Requirement for T cells or T-cell products for antigen-induced B-cell activation.", "content": "Although B lymphocytes can be triggered by B-cell mitogens and by certain other molecules to produce lymphokines, they do not produce lymphokines when stimulated with specific soluble protein antigens. We have investigated whether T-cell help would enable B cells to produce lymphokines when activated by antigens. Addition of small numbers of T cells to B-cell cultures resulted in significant production of a monocyte chemotactic factor. T cells could be replaced by supernates of antigen-stimulated T cells, demonstrating both that the chemotactic factor was B-cell-dervied and that T-cell help was mediated by a soluble factor. Although the T-cell factor was nonantigen specific, B-cell activation required the presence of both antigen and T-cell factor. Thus, it appears that although dependent upon T cells, B lymphocytes may play an important role in amplification of cell-mediated immune responses.", "contents": "Role of B lymphocytes in cell-mediated immunity. I. Requirement for T cells or T-cell products for antigen-induced B-cell activation. Although B lymphocytes can be triggered by B-cell mitogens and by certain other molecules to produce lymphokines, they do not produce lymphokines when stimulated with specific soluble protein antigens. We have investigated whether T-cell help would enable B cells to produce lymphokines when activated by antigens. Addition of small numbers of T cells to B-cell cultures resulted in significant production of a monocyte chemotactic factor. T cells could be replaced by supernates of antigen-stimulated T cells, demonstrating both that the chemotactic factor was B-cell-dervied and that T-cell help was mediated by a soluble factor. Although the T-cell factor was nonantigen specific, B-cell activation required the presence of both antigen and T-cell factor. Thus, it appears that although dependent upon T cells, B lymphocytes may play an important role in amplification of cell-mediated immune responses."}
{"id": "PMID:1086882", "title": "Regulatory mechanisms in cell-mediated immune responses. IV. Expression of a receptor for mixed lymphocyte reaction suppressor factor on activated T lymphocytes.", "content": "Suppression of the mixed lymphocyte reaction (MLR) by a soluble factor produced by alloantigen-activated spleen cells requires genetic homology between the factor-producing cells and responder cells in MLR. The ability of lymphocytes used as MLR responder cells to adsorb MLR suppressor factor was tested to investigate the expression of a receptor structure for suppressor molecules. Normal spleen or thymus cells had no effect on suppressor activity. Concanavalin A (Con A)-activated thymocytes, however, effectively removed suppressor activity, suggesting that the receptor is expressed only after activation and is not present or not functional on resting cells. Significantly neither phytohemagglutinin- nor lipopolysaccharide-activated lymphoid cells absorbed the factor. Furthermore, only Con A-activated thymocytes demonstrating genetic homology with the cell producing suppressor factor for H-2 regions to the right of I-E were effective absorbants. Alloantigen-stimulated spleen cells syngeneic to the suppressor cell also removed suppressor activity. These data support an hypothesis that subsequent to stimulation in MLR, T lymphocytes express a receptor, either through synthesis or alteration of an existing molecular structure, which then provides the appropriate site for interaction with suppressor molecules.", "contents": "Regulatory mechanisms in cell-mediated immune responses. IV. Expression of a receptor for mixed lymphocyte reaction suppressor factor on activated T lymphocytes. Suppression of the mixed lymphocyte reaction (MLR) by a soluble factor produced by alloantigen-activated spleen cells requires genetic homology between the factor-producing cells and responder cells in MLR. The ability of lymphocytes used as MLR responder cells to adsorb MLR suppressor factor was tested to investigate the expression of a receptor structure for suppressor molecules. Normal spleen or thymus cells had no effect on suppressor activity. Concanavalin A (Con A)-activated thymocytes, however, effectively removed suppressor activity, suggesting that the receptor is expressed only after activation and is not present or not functional on resting cells. Significantly neither phytohemagglutinin- nor lipopolysaccharide-activated lymphoid cells absorbed the factor. Furthermore, only Con A-activated thymocytes demonstrating genetic homology with the cell producing suppressor factor for H-2 regions to the right of I-E were effective absorbants. Alloantigen-stimulated spleen cells syngeneic to the suppressor cell also removed suppressor activity. These data support an hypothesis that subsequent to stimulation in MLR, T lymphocytes express a receptor, either through synthesis or alteration of an existing molecular structure, which then provides the appropriate site for interaction with suppressor molecules."}
{"id": "PMID:1086883", "title": "Allogeneic carrier-specific enhancement of hapten-specific secondary B-cell responses.", "content": "We have analyzed the capacity of carrier-specific T cells to enhance the immune response of hapten-specific secondary B cells which do not share genes in the H-2 complex with the T cells. For this analysis we have used the in vitro splenic focus technique which allows assessment of monoclonal responses of B cells isolated in splenic fragment cultures of irradiated reconstituted carrier primed mice. A previous report from this laboratory demonstrated that syngeny in the I region of the H-2 complex was necessary between collaborating hapten-specific primary (nonimmune) B cells and carrier-specific T cells for responses yielding IgG1 but not IgM antibody. These findings lead up to postulate that the expression of I-region gene products on the surface of primary B cells and I-region syngeny with collaborating carrier-specific T cells were essential elements in the triggering events leading to IgG1 synthesis by primary B cells. The results presented in the present report indicate that, unlike primary B cells, the majority of secondary B cells can be stimulated to produce IgG1 antibody in carrier-primed allogeneic recipients. Although the enhancement of secondary IgG1 responses is slightly greater with syngeneic T cells, the allogeneic collaborative interaction requires both carrier priming of recipient mice and stimulation with the homologous hapten-carrier complex and thus appears to be specific. These findings clearly discriminate secondary from primary B cells and indicate that the mechanism of stimulation of secondary B cells to yield IgG1-producing clones differs fundamentally from the stimulation of primary B cells in that the requisite for I-region syngeny is obviated.", "contents": "Allogeneic carrier-specific enhancement of hapten-specific secondary B-cell responses. We have analyzed the capacity of carrier-specific T cells to enhance the immune response of hapten-specific secondary B cells which do not share genes in the H-2 complex with the T cells. For this analysis we have used the in vitro splenic focus technique which allows assessment of monoclonal responses of B cells isolated in splenic fragment cultures of irradiated reconstituted carrier primed mice. A previous report from this laboratory demonstrated that syngeny in the I region of the H-2 complex was necessary between collaborating hapten-specific primary (nonimmune) B cells and carrier-specific T cells for responses yielding IgG1 but not IgM antibody. These findings lead up to postulate that the expression of I-region gene products on the surface of primary B cells and I-region syngeny with collaborating carrier-specific T cells were essential elements in the triggering events leading to IgG1 synthesis by primary B cells. The results presented in the present report indicate that, unlike primary B cells, the majority of secondary B cells can be stimulated to produce IgG1 antibody in carrier-primed allogeneic recipients. Although the enhancement of secondary IgG1 responses is slightly greater with syngeneic T cells, the allogeneic collaborative interaction requires both carrier priming of recipient mice and stimulation with the homologous hapten-carrier complex and thus appears to be specific. These findings clearly discriminate secondary from primary B cells and indicate that the mechanism of stimulation of secondary B cells to yield IgG1-producing clones differs fundamentally from the stimulation of primary B cells in that the requisite for I-region syngeny is obviated."}
{"id": "PMID:1086884", "title": "Isoantiserum-augmented development of lymphocyte-mediated cytotoxicity.", "content": "Passive administration of anti-P-814 isoantiserum (IS) with P-815 mastocytoma was shown to augment the development of T-lymphocyte-mediated cytotoxicity (LMC). The LMC activity augmented by IS was specific to immunizing tumor cells, but required the simultaneous administration of P-815 tumor cells and anti-P-815 serum, suggesting that the antigen-antibody complex is involved in the development of specific cytotoxic T cells. The serum component responsible for augmented development of LMC activity appeared to be IgM in that the augmenting activity fractionated in the void volume of a G-200 Sephadex column and appears very early after immunization. Our experimental results suggest the development of specific T-cell cytotoxicity can be directly regulated by specific IgM antibodies.", "contents": "Isoantiserum-augmented development of lymphocyte-mediated cytotoxicity. Passive administration of anti-P-814 isoantiserum (IS) with P-815 mastocytoma was shown to augment the development of T-lymphocyte-mediated cytotoxicity (LMC). The LMC activity augmented by IS was specific to immunizing tumor cells, but required the simultaneous administration of P-815 tumor cells and anti-P-815 serum, suggesting that the antigen-antibody complex is involved in the development of specific cytotoxic T cells. The serum component responsible for augmented development of LMC activity appeared to be IgM in that the augmenting activity fractionated in the void volume of a G-200 Sephadex column and appears very early after immunization. Our experimental results suggest the development of specific T-cell cytotoxicity can be directly regulated by specific IgM antibodies."}
{"id": "PMID:1086885", "title": "Role of self-carriers in the immune response and tolerance. I. B-cell unresponsiveness and cytotoxic T-cell immunity induced by haptenated syngeneic lymphoid cells.", "content": "Normal spleen cells cultured with TNP-modified syngeneic spleen cells fail to mount an anti-TNP PFC response to TNP-ficoll or TNP-red blood cells,but go on to generate cytotoxic T cells directed at hapten-modified H-2.These results suggest that hapten-modifeid spleen cells may differentially induce B-cell tolerance and T- (Ly 2,3) cell immunity. The differential response to modified self by lymphocyte subpopulations is discussed.", "contents": "Role of self-carriers in the immune response and tolerance. I. B-cell unresponsiveness and cytotoxic T-cell immunity induced by haptenated syngeneic lymphoid cells. Normal spleen cells cultured with TNP-modified syngeneic spleen cells fail to mount an anti-TNP PFC response to TNP-ficoll or TNP-red blood cells,but go on to generate cytotoxic T cells directed at hapten-modified H-2.These results suggest that hapten-modifeid spleen cells may differentially induce B-cell tolerance and T- (Ly 2,3) cell immunity. The differential response to modified self by lymphocyte subpopulations is discussed."}
{"id": "PMID:1086886", "title": "Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose.", "content": "T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation.", "contents": "Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose. T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation."}
{"id": "PMID:1086887", "title": "An oral test of pancreatic chymotrypsin activity using N-acetyl-L-tyrosyl-p-amino-benzoic acid in rats.", "content": "After a single dose of up to 50 mg/kg body weight (optimal amount for the test) of p-aminobenzoic acid or the corresponding amount of synthetic chymotrypsin substrate N-acetyl-L-tyrosyl-p-aminobenzoic acid, the urinary excretion of p-aminobenzoic acid in rats increases approximately linearly during 24 h. Higher doses do not cause any further significant increase in the amount of urine excreted p-aminobenzoic acid. Compared with a 24 h collecting period, 82% of p-aminobenzoic acid and 77% of p-aminobenzoic acid from N-acetyl-L-tyrosyl-p-aminobenzoic acid were excreted in the urine during the first 6 h after oral application of these substances. Therefore it is sufficient for practical purposes to determine p-aminobenzoic acid in 6 h urine samples. Stimulation of pancreatic secretion by application of 2.5 U of pancreozymin and 2.5 U of secretin failed to bring about significant increase in the cleaved p-aminobenzoic acid in the urine.", "contents": "An oral test of pancreatic chymotrypsin activity using N-acetyl-L-tyrosyl-p-amino-benzoic acid in rats. After a single dose of up to 50 mg/kg body weight (optimal amount for the test) of p-aminobenzoic acid or the corresponding amount of synthetic chymotrypsin substrate N-acetyl-L-tyrosyl-p-aminobenzoic acid, the urinary excretion of p-aminobenzoic acid in rats increases approximately linearly during 24 h. Higher doses do not cause any further significant increase in the amount of urine excreted p-aminobenzoic acid. Compared with a 24 h collecting period, 82% of p-aminobenzoic acid and 77% of p-aminobenzoic acid from N-acetyl-L-tyrosyl-p-aminobenzoic acid were excreted in the urine during the first 6 h after oral application of these substances. Therefore it is sufficient for practical purposes to determine p-aminobenzoic acid in 6 h urine samples. Stimulation of pancreatic secretion by application of 2.5 U of pancreozymin and 2.5 U of secretin failed to bring about significant increase in the cleaved p-aminobenzoic acid in the urine."}
{"id": "PMID:1086888", "title": "Strophanthidin-sensitive sodium fluxes in metabolically poisoned frog skeletal muscle.", "content": "Strophanthidin-sensitive and insensitive unidirectional fluxes of Na were measured in fog sartorius muscles whose internal Na levels were elevated by overnight storage in the cold. ATP levels were lowered, and ADP levels raised, by metabolic poisoning with either 2,4-dinitrofluorobenzene or iodoacetamide. Strophanthidin-sensitive Na efflux and influx both increased after poisoning, while strophanthidin-insensitives fluxes did not. The increase in efflux did not require the presence of external K but was greatly attenuated when Li replaced Na as the major external cation. Membrane potential was not markedly altered by 2,4-dinitrofluorobenzene. These observations indicate that the sodium pump of frog skeletal muscle resembles that of squid giant axon and human erythrocyte in its ability to catalyze Na-Na exchange to an extent determined by intracellular ATP/ADP levels.", "contents": "Strophanthidin-sensitive sodium fluxes in metabolically poisoned frog skeletal muscle. Strophanthidin-sensitive and insensitive unidirectional fluxes of Na were measured in fog sartorius muscles whose internal Na levels were elevated by overnight storage in the cold. ATP levels were lowered, and ADP levels raised, by metabolic poisoning with either 2,4-dinitrofluorobenzene or iodoacetamide. Strophanthidin-sensitive Na efflux and influx both increased after poisoning, while strophanthidin-insensitives fluxes did not. The increase in efflux did not require the presence of external K but was greatly attenuated when Li replaced Na as the major external cation. Membrane potential was not markedly altered by 2,4-dinitrofluorobenzene. These observations indicate that the sodium pump of frog skeletal muscle resembles that of squid giant axon and human erythrocyte in its ability to catalyze Na-Na exchange to an extent determined by intracellular ATP/ADP levels."}
{"id": "PMID:1086889", "title": "Neuroelectric blocking factors in human and animal sera evaluated using the isolated frog spinal cord.", "content": "The effects of sera from guinea-pigs with experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS) patients were evaluated and compared with the activity of control sera using the isolated frog spinal cord. Ventral root responses (VRR) were recorded during supramaximal ipsilateral dorsal root stimulation in the presence and absence of 25% serum. In control experiments with normal human and guinea-pig sera we observed a consistent, reversible increase in VRR averaging 20% and 17% respectively, and in no case was any significant decrease produced. In contrast, sera from EAE guinea-pigs 12 to 19 days after injection produced an equally rapid, reversible decrease in VRR. The decrease averaged 36% and was highly significant (P less than 0.0001) relative to controls. Similarly, sera from MS patients on the average decreased the VRR by 26%, and this again was significant compared with controls (P less than 10(-6).", "contents": "Neuroelectric blocking factors in human and animal sera evaluated using the isolated frog spinal cord. The effects of sera from guinea-pigs with experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS) patients were evaluated and compared with the activity of control sera using the isolated frog spinal cord. Ventral root responses (VRR) were recorded during supramaximal ipsilateral dorsal root stimulation in the presence and absence of 25% serum. In control experiments with normal human and guinea-pig sera we observed a consistent, reversible increase in VRR averaging 20% and 17% respectively, and in no case was any significant decrease produced. In contrast, sera from EAE guinea-pigs 12 to 19 days after injection produced an equally rapid, reversible decrease in VRR. The decrease averaged 36% and was highly significant (P less than 0.0001) relative to controls. Similarly, sera from MS patients on the average decreased the VRR by 26%, and this again was significant compared with controls (P less than 10(-6)."}
{"id": "PMID:1086890", "title": "Mechanism of the inverted supinator reflex. A clinical and neurophysiological study.", "content": "The inverted supinator reflex sign was analysed in two patients by recording the response of the finger flexor muscles of the forearm to tapping of the styloid process of the radius before and after procain infiltration of these muscles. It was found that the response persisted despite the block. The findings support the concept that the underlying mechanism is increased alpha motoneurone excitability below the level of the lesion; however, a possible contribution of the dynamic muscle spindles could not be excluded.", "contents": "Mechanism of the inverted supinator reflex. A clinical and neurophysiological study. The inverted supinator reflex sign was analysed in two patients by recording the response of the finger flexor muscles of the forearm to tapping of the styloid process of the radius before and after procain infiltration of these muscles. It was found that the response persisted despite the block. The findings support the concept that the underlying mechanism is increased alpha motoneurone excitability below the level of the lesion; however, a possible contribution of the dynamic muscle spindles could not be excluded."}
{"id": "PMID:1086891", "title": "A potential error in the quantitation of fecal blood loss: concise communication.", "content": "Chromium-51-labeled red cells were used to quantitate fecal blood loss in a patient with chronic upper gastrointestinal hemorrhage. On Day 1, the stool guaiac was positive but the blood loss indicated by 51Cr was less than 1 cm3. Blood loss in the stool by 51Cr did not become significant until Day 3, when it measured 23 cm3. The failure to detect abnormal blood loss on Day 1, and probably on Day 2, appears to be due to a long intestinal transit time from a proximal bleeding site. The problem of slow intestinal transit is not uncommon and could lead to a false-negative study or falsely low estimates of fecal blood loss. This problem could be minimized by beginning stool collection on Day 3 or by delaying stool collection until the appearance in the stool of an oral nonabsorbable marker swallowed when the 51Cr-tagged red cells are injected.", "contents": "A potential error in the quantitation of fecal blood loss: concise communication. Chromium-51-labeled red cells were used to quantitate fecal blood loss in a patient with chronic upper gastrointestinal hemorrhage. On Day 1, the stool guaiac was positive but the blood loss indicated by 51Cr was less than 1 cm3. Blood loss in the stool by 51Cr did not become significant until Day 3, when it measured 23 cm3. The failure to detect abnormal blood loss on Day 1, and probably on Day 2, appears to be due to a long intestinal transit time from a proximal bleeding site. The problem of slow intestinal transit is not uncommon and could lead to a false-negative study or falsely low estimates of fecal blood loss. This problem could be minimized by beginning stool collection on Day 3 or by delaying stool collection until the appearance in the stool of an oral nonabsorbable marker swallowed when the 51Cr-tagged red cells are injected."}
{"id": "PMID:1086895", "title": "Proctosigmoidoscopy and rectal biopsy in infants and children.", "content": "The medical records of all patients ages 0 to 21 years who underwent proctosigmoidoscopy and/or rectal biopsy over a 27 month period of time were reviewed to determine the efficacy and safety of these procedures in pediatric patients. One hundred twenty-one patients underwent proctosigmoidoscopy; 91 of these also had rectal biopsies. Median age was two years; 21% were less than six months and 8% less than one month of age. Depth of examination was 10 to 15 cm in most patients greater than 10 years of age. Induced friability was the most frequently observed mucosal abnormality. Abnormal findings were almost always present in patients with bloody diarrhea and were quite common in those with rectal bleeding, but less common in those with chronic diarrhea and abdominal pain. Colitis of various causes was the most common cause of blood in the stool; anal fissures were found in only four of 23 patients with rectal bleeding. Both proctosigmoidoscopy and rectal biopsy were needed to exclude the presence of colitis. Mobidity was 0% with proctosigmoidoscopy and 0.34% with rectal suction biopsy.", "contents": "Proctosigmoidoscopy and rectal biopsy in infants and children. The medical records of all patients ages 0 to 21 years who underwent proctosigmoidoscopy and/or rectal biopsy over a 27 month period of time were reviewed to determine the efficacy and safety of these procedures in pediatric patients. One hundred twenty-one patients underwent proctosigmoidoscopy; 91 of these also had rectal biopsies. Median age was two years; 21% were less than six months and 8% less than one month of age. Depth of examination was 10 to 15 cm in most patients greater than 10 years of age. Induced friability was the most frequently observed mucosal abnormality. Abnormal findings were almost always present in patients with bloody diarrhea and were quite common in those with rectal bleeding, but less common in those with chronic diarrhea and abdominal pain. Colitis of various causes was the most common cause of blood in the stool; anal fissures were found in only four of 23 patients with rectal bleeding. Both proctosigmoidoscopy and rectal biopsy were needed to exclude the presence of colitis. Mobidity was 0% with proctosigmoidoscopy and 0.34% with rectal suction biopsy."}
{"id": "PMID:1086900", "title": "Changes in membrane currents in bullfrog atrium produced by acetylcholine.", "content": "1. A double sucrose-gap voltage-clamp technique has been used to study the effects of acetylcholine on the membrane currents in atrial trabeculae of the bullfrog, Rana catesbeiana. 2. The second, or slow inward (Ca2+/Na+) current, was found to be markedly reduced by concentrations of acetylcholine greater than approximately 2-0 X 10(-8)M. The resulting decrease in net calcium entry provides a straightforward explanation for the negative inotropic action of acetylcholine in atrial muscle. 3. Measurements of membrane resistance near the resting potential showed that relatively high doses of acetylcholine (approximately 10(-7) M) decrease membrane resistance by about twofold. This effect is shown to be the result of an increase in a time-independent background current which appears to be carried mainly by potassium ions. 4. Using appropriate pharmacological techniques, it has been possible to demonstrate: (i) that the peak slow inward current is reduced to about half its initial value before any significant increase in background current occurs; (ii) that even when a sufficient dose of acetylcholine to produce an increase in background current is used, the background current shows inward-going rectification and cannot account for the observed reduction in the slow inward current. 5. No consistent change was observed in the degree of activation of the time-dependent outward membrane currents after application of concentrations of acetylcholine which produced large decreases in the peak slow inward current. 6. These results are discussed in relation to previous electro-physiological and radioisotope studies of the mechanism of the negative inotropic effect of acetylcholine in cardiac muscle.", "contents": "Changes in membrane currents in bullfrog atrium produced by acetylcholine. 1. A double sucrose-gap voltage-clamp technique has been used to study the effects of acetylcholine on the membrane currents in atrial trabeculae of the bullfrog, Rana catesbeiana. 2. The second, or slow inward (Ca2+/Na+) current, was found to be markedly reduced by concentrations of acetylcholine greater than approximately 2-0 X 10(-8)M. The resulting decrease in net calcium entry provides a straightforward explanation for the negative inotropic action of acetylcholine in atrial muscle. 3. Measurements of membrane resistance near the resting potential showed that relatively high doses of acetylcholine (approximately 10(-7) M) decrease membrane resistance by about twofold. This effect is shown to be the result of an increase in a time-independent background current which appears to be carried mainly by potassium ions. 4. Using appropriate pharmacological techniques, it has been possible to demonstrate: (i) that the peak slow inward current is reduced to about half its initial value before any significant increase in background current occurs; (ii) that even when a sufficient dose of acetylcholine to produce an increase in background current is used, the background current shows inward-going rectification and cannot account for the observed reduction in the slow inward current. 5. No consistent change was observed in the degree of activation of the time-dependent outward membrane currents after application of concentrations of acetylcholine which produced large decreases in the peak slow inward current. 6. These results are discussed in relation to previous electro-physiological and radioisotope studies of the mechanism of the negative inotropic effect of acetylcholine in cardiac muscle."}
{"id": "PMID:1086901", "title": "Action of vinblastine on the spontaneous release of acetylcholine at the frog neuromuscular junction.", "content": "1. Vinblastine induces reversible changes of the spontaneous release of acetylcholine (ACh) at the frog neuromuscular junction as characterized by the appearance of giant potentials. These large potentials occur soon after soaking the muscle in vinblastine and are not consequent to a large increase in the frequency of spontaneous release. Their number, relative to the total number of spontaneous potentials, increases with the duration of soaking. 2. Large potentials appear even in the presence of tetrodotoxin or in low Ca2+-high Mg2+ Ringer solution. 3. Following vinblastine treatment,the amplitude histogram of spontaneous potentials recorded from a number of fibres display an evident periodicity with peaks occurring regularly at simple multiples of the model amplitude of the unitary potentials. It is suggested that giant potentials are produced by the release of preformed pluriquantal packets of ACh. 4. Comparison of the amplitude distribution of spontaneous potentials and end-plates potentials show that only an insignificant number of large quanta are released by nerve stimuli. The absolute frequency of giant potentials does not markedly change when spontaneous discharge is accelerated by hypertonic solution. 5. The mechanism by which vinblastine induces the appearance of giant potentials is discussed.", "contents": "Action of vinblastine on the spontaneous release of acetylcholine at the frog neuromuscular junction. 1. Vinblastine induces reversible changes of the spontaneous release of acetylcholine (ACh) at the frog neuromuscular junction as characterized by the appearance of giant potentials. These large potentials occur soon after soaking the muscle in vinblastine and are not consequent to a large increase in the frequency of spontaneous release. Their number, relative to the total number of spontaneous potentials, increases with the duration of soaking. 2. Large potentials appear even in the presence of tetrodotoxin or in low Ca2+-high Mg2+ Ringer solution. 3. Following vinblastine treatment,the amplitude histogram of spontaneous potentials recorded from a number of fibres display an evident periodicity with peaks occurring regularly at simple multiples of the model amplitude of the unitary potentials. It is suggested that giant potentials are produced by the release of preformed pluriquantal packets of ACh. 4. Comparison of the amplitude distribution of spontaneous potentials and end-plates potentials show that only an insignificant number of large quanta are released by nerve stimuli. The absolute frequency of giant potentials does not markedly change when spontaneous discharge is accelerated by hypertonic solution. 5. The mechanism by which vinblastine induces the appearance of giant potentials is discussed."}
{"id": "PMID:1086902", "title": "Current-voltage relationships in the crystalline lens.", "content": "1. Electrical coupling between the cells of the crystalline lens of the frog eye was studied using two intralenticular micro-electrodes, one to pass current and one to record potential. In most experiments, both electrodes were placed just inside the posterior surface of the lens at a depth of approximately 200 mum from the surface. Step functions of current were applied and the time course of the resulting change in voltage was measured at many different electrode separations. 2. The voltage change has both a fast component, which occurs only locally in the region close to the current passing micro-electrode, and a slow component, which is spatially uniform, independent of distance from the current micro-electrode. 3. This behaviour is predicted by an electrical model of a single large spherical cell, and so that model can be used to analyse our data. 4. The resistivity of the lens 'interior' (both cytoplasm and coupling resistivity) is 625 omega cm; the resistance of the lens 'membrane' is 2751 omega cm2. 5. The data and analysis help to reconcile discrepancies between previous measurements of the electrical properties of the lens and show clearly that there is substantial electrical coupling from cell to cell. The method should allow investigation of the role of electrical coupling in cataract formation in the crystalline lens.", "contents": "Current-voltage relationships in the crystalline lens. 1. Electrical coupling between the cells of the crystalline lens of the frog eye was studied using two intralenticular micro-electrodes, one to pass current and one to record potential. In most experiments, both electrodes were placed just inside the posterior surface of the lens at a depth of approximately 200 mum from the surface. Step functions of current were applied and the time course of the resulting change in voltage was measured at many different electrode separations. 2. The voltage change has both a fast component, which occurs only locally in the region close to the current passing micro-electrode, and a slow component, which is spatially uniform, independent of distance from the current micro-electrode. 3. This behaviour is predicted by an electrical model of a single large spherical cell, and so that model can be used to analyse our data. 4. The resistivity of the lens 'interior' (both cytoplasm and coupling resistivity) is 625 omega cm; the resistance of the lens 'membrane' is 2751 omega cm2. 5. The data and analysis help to reconcile discrepancies between previous measurements of the electrical properties of the lens and show clearly that there is substantial electrical coupling from cell to cell. The method should allow investigation of the role of electrical coupling in cataract formation in the crystalline lens."}
{"id": "PMID:1086903", "title": "The role of some small peptides in the transfer of amino nitrogen across the wall of vascularly perfused intestine.", "content": "The characteristics have been investigated of the transfer into the vascular bed of L-leucine and glycine from free amino acids or peptides in the intestinal lumen of Rana pipiens. Over the concentration range 0-5-10 mM the transfer of L-leucine is but little affected by the presence of equimolar concentrations of glycine but the transfer of glycine, in contrast, is greatly inhibited by the presence of L-leucine. 2. With glycyl-L-leucine in the intestinal lumen, the rate of transfer of glycine into the vascular bed is much greater than from the mixture of the two amino acids and is equal to that of the L-leucine. From L-leucyl-glycine the rates of transfer of leucine and of glycine are also higher than from the mixture of the two amino acids but the rate of transfer of glycine is somewhat lower than that of leucine. There is no evidence of the presence of the dipeptides in the effluent from the portal vein. 3. When the peptide glycyl-L-leucine is added to the lumen in the presence of 10 mM concentrations of the free amino acids, additional amounts of L-leucine and of glycine are transferred in approximately equimolar quantities into the vascular bed. This additional transfer exhibits saturation with respect to concentration of peptide in the intestinal lumen. An additional transfer of amino acids was also found when L-leucyl-glycine was added to the lumen in the presence of saturating concentrations of the two amino acids. 4. Evidence is presented that the presence of the dipeptides in the intestinal lumen had little effect on the transfer of free amino acids from the lumen into the vascular bed. Although the transfer of free amino acids from the lumen into the vascular bed is significantly, but not completely, abolished when the Na in the intestinal lumen is replaced by K, the transfer of the amino acids from the dipeptides is but little affected. 5. The findings are discussed in relation to the view that the dipeptides are transported into the mucosal epithelium by a process that is distinct from those promoting uptake of the individual amino acids. Being completely hydrolysed, there is no evidence for an accumulative uptake of the peptides; it is suggested that this may be related to the fact that the peptide uptake occurs in the absence of Na in the intestinal lumen.", "contents": "The role of some small peptides in the transfer of amino nitrogen across the wall of vascularly perfused intestine. The characteristics have been investigated of the transfer into the vascular bed of L-leucine and glycine from free amino acids or peptides in the intestinal lumen of Rana pipiens. Over the concentration range 0-5-10 mM the transfer of L-leucine is but little affected by the presence of equimolar concentrations of glycine but the transfer of glycine, in contrast, is greatly inhibited by the presence of L-leucine. 2. With glycyl-L-leucine in the intestinal lumen, the rate of transfer of glycine into the vascular bed is much greater than from the mixture of the two amino acids and is equal to that of the L-leucine. From L-leucyl-glycine the rates of transfer of leucine and of glycine are also higher than from the mixture of the two amino acids but the rate of transfer of glycine is somewhat lower than that of leucine. There is no evidence of the presence of the dipeptides in the effluent from the portal vein. 3. When the peptide glycyl-L-leucine is added to the lumen in the presence of 10 mM concentrations of the free amino acids, additional amounts of L-leucine and of glycine are transferred in approximately equimolar quantities into the vascular bed. This additional transfer exhibits saturation with respect to concentration of peptide in the intestinal lumen. An additional transfer of amino acids was also found when L-leucyl-glycine was added to the lumen in the presence of saturating concentrations of the two amino acids. 4. Evidence is presented that the presence of the dipeptides in the intestinal lumen had little effect on the transfer of free amino acids from the lumen into the vascular bed. Although the transfer of free amino acids from the lumen into the vascular bed is significantly, but not completely, abolished when the Na in the intestinal lumen is replaced by K, the transfer of the amino acids from the dipeptides is but little affected. 5. The findings are discussed in relation to the view that the dipeptides are transported into the mucosal epithelium by a process that is distinct from those promoting uptake of the individual amino acids. Being completely hydrolysed, there is no evidence for an accumulative uptake of the peptides; it is suggested that this may be related to the fact that the peptide uptake occurs in the absence of Na in the intestinal lumen."}
{"id": "PMID:1086904", "title": "Studies in the neurophysiology of learning: X. Phase coherence of brain potentials as index of alertness and orienting response in the rat.", "content": "With use of electrical potentials derived from chronically implanted electrodes in the rat's forebrain to trigger a computer of average transients (CAT), averaged traces were obtained which gave characteristic undulations under conditions of arousal and alertness (N = seven albino rats--six male and one female). Under conditions of extreme adaptation to the environmental condition, the averaged undulations disappeared. The undulations were characterized by a period of 125-150 milliseconds and occurred in trains of .3-.5 second. The parameters of the waves were constant for a variety of novel environmental conditions, but the waves vanished in those conditions that appeared to greatly stress or terrify the rat. The undulations so obtained were conditionable to initially neutral stimuli in both the forward and backward directions.", "contents": "Studies in the neurophysiology of learning: X. Phase coherence of brain potentials as index of alertness and orienting response in the rat. With use of electrical potentials derived from chronically implanted electrodes in the rat's forebrain to trigger a computer of average transients (CAT), averaged traces were obtained which gave characteristic undulations under conditions of arousal and alertness (N = seven albino rats--six male and one female). Under conditions of extreme adaptation to the environmental condition, the averaged undulations disappeared. The undulations were characterized by a period of 125-150 milliseconds and occurred in trains of .3-.5 second. The parameters of the waves were constant for a variety of novel environmental conditions, but the waves vanished in those conditions that appeared to greatly stress or terrify the rat. The undulations so obtained were conditionable to initially neutral stimuli in both the forward and backward directions."}
{"id": "PMID:1086905", "title": "Lymphocytes forming rosettes with sheep erythrocytes in metastatic pleural effusions.", "content": "Percentages of lymphocytes forming rosettes with sheep erythrocytes at 29 degrees C were determined in 10 cancer patients with metastases to the pleural cavity. Compared with normal controls, the patients showed a decreased proportion of rosette-forming cells (RFC) in the peripheral blood. The same patients had elevated levels of RFC in their metastatic pleural effusions. However, 2 patients with benign diseases had normal levels of RFC in their peripheral blood and pleural transudates. These observations suggested that in cancer patients some T-cells might migrate from the peripheral blood and accumulate in sites of tumor infiltration such as the pleural cavity.", "contents": "Lymphocytes forming rosettes with sheep erythrocytes in metastatic pleural effusions. Percentages of lymphocytes forming rosettes with sheep erythrocytes at 29 degrees C were determined in 10 cancer patients with metastases to the pleural cavity. Compared with normal controls, the patients showed a decreased proportion of rosette-forming cells (RFC) in the peripheral blood. The same patients had elevated levels of RFC in their metastatic pleural effusions. However, 2 patients with benign diseases had normal levels of RFC in their peripheral blood and pleural transudates. These observations suggested that in cancer patients some T-cells might migrate from the peripheral blood and accumulate in sites of tumor infiltration such as the pleural cavity."}
{"id": "PMID:1086906", "title": "Regulatory variants for the expression of H-2 antigens. II. Level of discrimination of cell-mediated cytolysis.", "content": "We used alloantigenic variants to analyze whether killer T-cells and antibody in the presence of complement (two effector components of the immune system) recognized the same determinants on target tissues. The variants were selected from mouse myeloma cells by immune selection and differed from the parent cell in that they did not express H-2 antigens. The results suggested that both killer T-cells and humoral effectors recognized the same determinants, though the killer T-cells may have been more sensitive either in recognizing these determinants or mediating lysis.", "contents": "Regulatory variants for the expression of H-2 antigens. II. Level of discrimination of cell-mediated cytolysis. We used alloantigenic variants to analyze whether killer T-cells and antibody in the presence of complement (two effector components of the immune system) recognized the same determinants on target tissues. The variants were selected from mouse myeloma cells by immune selection and differed from the parent cell in that they did not express H-2 antigens. The results suggested that both killer T-cells and humoral effectors recognized the same determinants, though the killer T-cells may have been more sensitive either in recognizing these determinants or mediating lysis."}
{"id": "PMID:1086909", "title": "The probability of closure in aortocoronary vein bypass grafts.", "content": "A study of 540 patients who had the aortocoronary vein bypass operation (from several transplant teams) showed that grafts within a patient closed independently of one another during the first-two year postoperative period. For patients with a single vein graft, there was a 23% chance of closure during the first two year postoperative period. The chance that all grafts would close during this period for patients with two grafts was 5%, and it was 1% for patients with three grafts. The knowledge of these closure probabilities may be used by the surgeon when planning the optimal surgical maneuver.", "contents": "The probability of closure in aortocoronary vein bypass grafts. A study of 540 patients who had the aortocoronary vein bypass operation (from several transplant teams) showed that grafts within a patient closed independently of one another during the first-two year postoperative period. For patients with a single vein graft, there was a 23% chance of closure during the first two year postoperative period. The chance that all grafts would close during this period for patients with two grafts was 5%, and it was 1% for patients with three grafts. The knowledge of these closure probabilities may be used by the surgeon when planning the optimal surgical maneuver."}
{"id": "PMID:1086910", "title": "Antihemophilic factor concentrate therapy in von Willebrand disease. Dissociation of bleeding-time factor and ristocetin-cofactor activities.", "content": "Treatment of von Willebrand disease with two plasma antihemophilic factor (AHF) concentrates, cryoprecipitate and glycine-precipitated AHF, was compared. Both concentrates were equally effective in immediately raising the plasma levels of factor VIII, the factor VIII-related antigen, and the ristocetin-related von Willebrand factor (vWF) and in stimulating a secondary rise in plasma factor VIII. Given either concentrate, the vWF activity, the antigen, and factor VIII levels were normalized in a patient with von Willebrand disease. However, correction of the prolonged bleeding time and control of bleeding occurred only with the cryoprecipitate. The bleeding-time corrective factor and the ristocetin-related vWF or platelet-aggregating factor are dissociable, distinct activites.", "contents": "Antihemophilic factor concentrate therapy in von Willebrand disease. Dissociation of bleeding-time factor and ristocetin-cofactor activities. Treatment of von Willebrand disease with two plasma antihemophilic factor (AHF) concentrates, cryoprecipitate and glycine-precipitated AHF, was compared. Both concentrates were equally effective in immediately raising the plasma levels of factor VIII, the factor VIII-related antigen, and the ristocetin-related von Willebrand factor (vWF) and in stimulating a secondary rise in plasma factor VIII. Given either concentrate, the vWF activity, the antigen, and factor VIII levels were normalized in a patient with von Willebrand disease. However, correction of the prolonged bleeding time and control of bleeding occurred only with the cryoprecipitate. The bleeding-time corrective factor and the ristocetin-related vWF or platelet-aggregating factor are dissociable, distinct activites."}
{"id": "PMID:1086913", "title": "Polyene antibiotics. VII. Carbon-13 nuclear magnetic resonance evidence for cyclic hemiketals in the polyene antibiotics amphotericin B, nystatin A1, tetrin A, tetrin B, lucensomycin, and pimaricin1,2.", "content": "Carbon magnetic resonance establishes conclusively that six polyene macrolide antibiotics containing keto groups (the heptaene amphotericin B, the tetraene-diene nystatin A1, and the tetraenes tetrin A, tetrin B, pimaricin, and lucensomycin) exist in the hemiketal form in solution. Their spectra all contain a hemiketal carbon's absorption near 97 ppm but lack a keto carbon's absorption near 210 ppm. The non-polyenic macrolide erythromycin, on the other hand, exists in the keto form.", "contents": "Polyene antibiotics. VII. Carbon-13 nuclear magnetic resonance evidence for cyclic hemiketals in the polyene antibiotics amphotericin B, nystatin A1, tetrin A, tetrin B, lucensomycin, and pimaricin1,2. Carbon magnetic resonance establishes conclusively that six polyene macrolide antibiotics containing keto groups (the heptaene amphotericin B, the tetraene-diene nystatin A1, and the tetraenes tetrin A, tetrin B, pimaricin, and lucensomycin) exist in the hemiketal form in solution. Their spectra all contain a hemiketal carbon's absorption near 97 ppm but lack a keto carbon's absorption near 210 ppm. The non-polyenic macrolide erythromycin, on the other hand, exists in the keto form."}
{"id": "PMID:1086915", "title": "Cytotoxic activity of purified guinea pig lymphotoxin against various cell lines.", "content": "Cytotoxic activity of the purified guinea pig lymphotoxin (GLT) was tested on various blood cells and various cultured cell lines. GLT was generally ineffective on erythrocytes and lymphocytes, but it was somewhat effective on peritoneal exudate cells indicating GLT might play an important role in the regulatory mechanism of the cellular immunity. Considerable difference in target cell sensitivity was observed among the cultured cell lines tested. The sensitivity of L cells to GLT was significantly lowered by the trypsin treatment of the cells. Furthermore, porcine thyroglobulin glycopeptide exerted weak but definite inhibitory activity against GLT. These results suggest that the cytotoxicity of GLT might be triggered by the binding of GLT to the receptor sites of glycoprotein nature on the cell surface.", "contents": "Cytotoxic activity of purified guinea pig lymphotoxin against various cell lines. Cytotoxic activity of the purified guinea pig lymphotoxin (GLT) was tested on various blood cells and various cultured cell lines. GLT was generally ineffective on erythrocytes and lymphocytes, but it was somewhat effective on peritoneal exudate cells indicating GLT might play an important role in the regulatory mechanism of the cellular immunity. Considerable difference in target cell sensitivity was observed among the cultured cell lines tested. The sensitivity of L cells to GLT was significantly lowered by the trypsin treatment of the cells. Furthermore, porcine thyroglobulin glycopeptide exerted weak but definite inhibitory activity against GLT. These results suggest that the cytotoxicity of GLT might be triggered by the binding of GLT to the receptor sites of glycoprotein nature on the cell surface."}
{"id": "PMID:1086916", "title": "Carcinogenesis in tissue culture 27: heterotransplantation as an alternative in vivo assay for neoplastic state of cells in culture.", "content": "Cells of serially cultured strains and newly-isolated cells originating from rat liver and lung were heterotransplanted into pouches of golden hamsters treated with antithymocyte serum (ATS). The \"take\" was determined by histopathological examination of nodules formed by 3 weeks after the transplantation. The results obtained under this criteria showed that 9 of 11 cell strains backtransplantable to rats and 2 of 4 strains untransplantable were in parallel with those in rats. Three kinds of uncultured liver cells resulted in \"no take\".", "contents": "Carcinogenesis in tissue culture 27: heterotransplantation as an alternative in vivo assay for neoplastic state of cells in culture. Cells of serially cultured strains and newly-isolated cells originating from rat liver and lung were heterotransplanted into pouches of golden hamsters treated with antithymocyte serum (ATS). The \"take\" was determined by histopathological examination of nodules formed by 3 weeks after the transplantation. The results obtained under this criteria showed that 9 of 11 cell strains backtransplantable to rats and 2 of 4 strains untransplantable were in parallel with those in rats. Three kinds of uncultured liver cells resulted in \"no take\"."}
{"id": "PMID:1086927", "title": "[Atypical retinopathia pigmentosa with central retinoschisis (Goldmann-Favre) (author's transl)].", "content": "A family is presented whose two daughters suffer from Goldmann-Favre disease. They have a retinoschisis and bone corpuscle like pigmentations. Both the girls have pathological ERG's (reduced b-waves, potentials up to 40 muV, no a-waves). The EOG of the younger is normal (Arden ratio 200%), but the EOG of the elder sister is pathological (Arden ratio right 120%, left 150%).", "contents": "[Atypical retinopathia pigmentosa with central retinoschisis (Goldmann-Favre) (author's transl)]. A family is presented whose two daughters suffer from Goldmann-Favre disease. They have a retinoschisis and bone corpuscle like pigmentations. Both the girls have pathological ERG's (reduced b-waves, potentials up to 40 muV, no a-waves). The EOG of the younger is normal (Arden ratio 200%), but the EOG of the elder sister is pathological (Arden ratio right 120%, left 150%)."}
{"id": "PMID:1086928", "title": "Cellular immune response in vitro: I. A requirement for time-dependent T-lymphocyte cell division of cytotoxic cells in the allogeneic response.", "content": "Immunocompetent lymphoid cells cultured in vitro with allogeneic stimulator cells have been shown to produce T-lymphocyte populations which are specifically cytotoxic in vitro to the stimulatory cells whether normal or malignant. Although the culture requirements as well as the allogeneic requirements are known, the events leading to the production of T-lymphocyte cytotoxic cells is poorly understood. This study examines the role of cell division in the production of allogeneic cytotoxic T-cells in vitro. The elimination of cell division during the first 24 hr of allogeneic culture does not affect the cell-mediated cytotoxic immune response in vitro. Cell division is required, however, from 24 hr through 96 hr in culture and not necessary after 96 hr.", "contents": "Cellular immune response in vitro: I. A requirement for time-dependent T-lymphocyte cell division of cytotoxic cells in the allogeneic response. Immunocompetent lymphoid cells cultured in vitro with allogeneic stimulator cells have been shown to produce T-lymphocyte populations which are specifically cytotoxic in vitro to the stimulatory cells whether normal or malignant. Although the culture requirements as well as the allogeneic requirements are known, the events leading to the production of T-lymphocyte cytotoxic cells is poorly understood. This study examines the role of cell division in the production of allogeneic cytotoxic T-cells in vitro. The elimination of cell division during the first 24 hr of allogeneic culture does not affect the cell-mediated cytotoxic immune response in vitro. Cell division is required, however, from 24 hr through 96 hr in culture and not necessary after 96 hr."}
{"id": "PMID:1086931", "title": "Histologic changes in saphenous vein aorta-coronary bypass grafts. The effect of the angle of the aortic anastomosis.", "content": "Thirteen dogs were subjected to bypass grafting from the aorta to the left circumflex coronary artery with the saphenous vein to determine whether the angle of insertion of the saphenous vein into the aorta influences the functional and histologic fate of the grafts. The angle of the aortic anastomosis was obtuse in 6 dogs, acute in 5, and perpendicular in 2. Histologic examination of all 13 grafts 6 to 19 months (mean 9.4) postoperatively showed fibrous intimal proliferative lesions of variable severity along the entire length of the grafts, occasionally with extension into the native coronary arteries. Loss of medical smooth muscle and adventitial fibrosis also occurred in all 13 grafts. The extent and severity of these changes, however, were not related to the angle aortic anastomosis.", "contents": "Histologic changes in saphenous vein aorta-coronary bypass grafts. The effect of the angle of the aortic anastomosis. Thirteen dogs were subjected to bypass grafting from the aorta to the left circumflex coronary artery with the saphenous vein to determine whether the angle of insertion of the saphenous vein into the aorta influences the functional and histologic fate of the grafts. The angle of the aortic anastomosis was obtuse in 6 dogs, acute in 5, and perpendicular in 2. Histologic examination of all 13 grafts 6 to 19 months (mean 9.4) postoperatively showed fibrous intimal proliferative lesions of variable severity along the entire length of the grafts, occasionally with extension into the native coronary arteries. Loss of medical smooth muscle and adventitial fibrosis also occurred in all 13 grafts. The extent and severity of these changes, however, were not related to the angle aortic anastomosis."}
{"id": "PMID:1086932", "title": "Late cardiac tamponade: a potentially lethal complication of open-heart surgery.", "content": "Cardiac tamponade occurring late after open-heart surgery is a frequently fatal complication. Of the 13 cases previously reported in the literature, there were seven deaths. In this report, three additional patients with late cardiac tamponade successfully treated are presented. Postoperative anticoagulation with Coumadin appeared to contribute to this complication. Increased awareness of late cardiac tamponade allowing prompt recognition and immediate decompression of the pericardial sac should lower the high mortality rate previously associated with the condition.", "contents": "Late cardiac tamponade: a potentially lethal complication of open-heart surgery. Cardiac tamponade occurring late after open-heart surgery is a frequently fatal complication. Of the 13 cases previously reported in the literature, there were seven deaths. In this report, three additional patients with late cardiac tamponade successfully treated are presented. Postoperative anticoagulation with Coumadin appeared to contribute to this complication. Increased awareness of late cardiac tamponade allowing prompt recognition and immediate decompression of the pericardial sac should lower the high mortality rate previously associated with the condition."}
{"id": "PMID:1086934", "title": "Host factors in chronic obstructive pulmonary disease in an upper Midwest rural community. Design, case selection, and clinical characteristics in a matched-pair study.", "content": "A series of 111 index subjects with chronic obstructive pulmonary disease (COPD) who had forced expiratory volume in 1 second (FEV1) of 70% or less of that predicted were matched on the basis of age, sex, occupation, and smoking history with control subjects who had an FEV1 of 85% or more of that predicted. Index and control subjects with seasonal or reversible airway disease were excluded. Men outnumbered women by a ratio of 4.5 to 1. Thirty-five percent of the women and 2% of the men were nonsmokers (0 pack-years). There were three PiZ phenotypes in the index group (two nonsmokers) and none in the controls. PiMZ phenotypes in the index group outnumbered those in the controls by 8 to 5. Host factors that might be important in these closely matched pairs were sought by history, physical examination, and a large battery of laboratory tests. A standard respiratory questionnaire revealed the anticipated significantly higher frequency of cough, phlegm, noisy respiration, and all grades of dyspnea in index subjects. Previous lower respiratory tract infections also were more frequent in index subjects than in controls. There were no detectable differences between groups in the frequency of upper airway infections, nasal polyps, sinus surgery, or reported allergy to any substance. If the British Medical Research Council's definition of chronic bronchitis were applied to our study, about two-thirds of our index subjects and almost one-third of our controls would be considered to have chronic bronchitis. Pack-years of smoking were not significantly associated with the amount and duration of cough and expectoration in male or female index subjects or controls. Significant differences between index and control groups on physical examination included the audible forced expiratory flow time over the trachea, the estimated maximal midexpiratory flow, breath sounds, rales, and total excursion of the hemidiaphragms. An endocrine questionnaire and measurement of blood sex hormones did not give any clues as to the propensity of males to develop COPD. Women with airway obstruction similar to that of men had histories of significantly fewer pack-years than did the men, and there was a much larger proportion of women who never smoked. Further studies, specifically on genetic and immunologic characteristics, are under way to identify potential host factors.", "contents": "Host factors in chronic obstructive pulmonary disease in an upper Midwest rural community. Design, case selection, and clinical characteristics in a matched-pair study. A series of 111 index subjects with chronic obstructive pulmonary disease (COPD) who had forced expiratory volume in 1 second (FEV1) of 70% or less of that predicted were matched on the basis of age, sex, occupation, and smoking history with control subjects who had an FEV1 of 85% or more of that predicted. Index and control subjects with seasonal or reversible airway disease were excluded. Men outnumbered women by a ratio of 4.5 to 1. Thirty-five percent of the women and 2% of the men were nonsmokers (0 pack-years). There were three PiZ phenotypes in the index group (two nonsmokers) and none in the controls. PiMZ phenotypes in the index group outnumbered those in the controls by 8 to 5. Host factors that might be important in these closely matched pairs were sought by history, physical examination, and a large battery of laboratory tests. A standard respiratory questionnaire revealed the anticipated significantly higher frequency of cough, phlegm, noisy respiration, and all grades of dyspnea in index subjects. Previous lower respiratory tract infections also were more frequent in index subjects than in controls. There were no detectable differences between groups in the frequency of upper airway infections, nasal polyps, sinus surgery, or reported allergy to any substance. If the British Medical Research Council's definition of chronic bronchitis were applied to our study, about two-thirds of our index subjects and almost one-third of our controls would be considered to have chronic bronchitis. Pack-years of smoking were not significantly associated with the amount and duration of cough and expectoration in male or female index subjects or controls. Significant differences between index and control groups on physical examination included the audible forced expiratory flow time over the trachea, the estimated maximal midexpiratory flow, breath sounds, rales, and total excursion of the hemidiaphragms. An endocrine questionnaire and measurement of blood sex hormones did not give any clues as to the propensity of males to develop COPD. Women with airway obstruction similar to that of men had histories of significantly fewer pack-years than did the men, and there was a much larger proportion of women who never smoked. Further studies, specifically on genetic and immunologic characteristics, are under way to identify potential host factors."}
{"id": "PMID:1086935", "title": "Incidence of iron deficiency anemia in patients with large diaphragmatic hernia. A controlled study.", "content": "The incidence of anemia in 259 patients with a diaphragmatic hernia large enough to be seen on a routine chest roentgenogram was compared with that in 259 age- and sex-matched controls. Eighteen patients with diaphragmatic hernia were anemic, compared to one control subject (P less than 0.001). In thirteen patients with diaphragmatic hernia and in one control the anemia was proven to be caused by iron deficiency. The findings provide additional evidence that a large diaphragmatic hernia can cause anemia secondary to chronic gastrointestinal blood loss, which is usually not the result of reflux esophagitis.", "contents": "Incidence of iron deficiency anemia in patients with large diaphragmatic hernia. A controlled study. The incidence of anemia in 259 patients with a diaphragmatic hernia large enough to be seen on a routine chest roentgenogram was compared with that in 259 age- and sex-matched controls. Eighteen patients with diaphragmatic hernia were anemic, compared to one control subject (P less than 0.001). In thirteen patients with diaphragmatic hernia and in one control the anemia was proven to be caused by iron deficiency. The findings provide additional evidence that a large diaphragmatic hernia can cause anemia secondary to chronic gastrointestinal blood loss, which is usually not the result of reflux esophagitis."}
{"id": "PMID:1086938", "title": "[The portacaval anastomosis (author's transl)].", "content": "All patients treated in the authors' clinic for portal hypertension from 1958 to 1975 are reviewed and the problems encountered with portacaval anastomoses are explained with special reference to encephalopathy and hepatic failure. Intrahepatic block was recorded in 88% of 722 patients. Portacaval anastomoses were constructed in 292 cases, splenorenal anastomoses in 175, and mesenterico-caval anastomoses in 12. Among the patients with stationary  cirrhosis, the 6-year survival was unrelated to the type of operation performed and amounted to approximately 70%, while 6-year survival was only 40% in patients with progressive cirrhosis of the liver, whether they received surgical or conservative treatment.", "contents": "[The portacaval anastomosis (author's transl)]. All patients treated in the authors' clinic for portal hypertension from 1958 to 1975 are reviewed and the problems encountered with portacaval anastomoses are explained with special reference to encephalopathy and hepatic failure. Intrahepatic block was recorded in 88% of 722 patients. Portacaval anastomoses were constructed in 292 cases, splenorenal anastomoses in 175, and mesenterico-caval anastomoses in 12. Among the patients with stationary  cirrhosis, the 6-year survival was unrelated to the type of operation performed and amounted to approximately 70%, while 6-year survival was only 40% in patients with progressive cirrhosis of the liver, whether they received surgical or conservative treatment."}
{"id": "PMID:1086939", "title": "[Subcutaneous transposition of the spleen: an experimental and clinical study (author's transl)].", "content": "The growing scepticism about the use of portocaval anastomosis operations to prevent renewed bleeding of esophageal varices has led to the testing of new methods. This report describes subcutaneous transposition of the resected spleen in normal experimental animals with the Vena porta constricted with Ameroid rings and liver cirrhosis induced by DMNA. The report also covers 9 patients operated on from 1972 to 1973 with subcutaneous transposition of the spleen. A 77-year-old woman died postoperatively, one patient died after 2 years 4 months, and one had slight bleeding. All the other patients are alive 2-4 years after the operation, with no signs of renewed bleeding. The advantage of subcutaneous transposition of the spleen is that it eliminates hypersplenism.", "contents": "[Subcutaneous transposition of the spleen: an experimental and clinical study (author's transl)]. The growing scepticism about the use of portocaval anastomosis operations to prevent renewed bleeding of esophageal varices has led to the testing of new methods. This report describes subcutaneous transposition of the resected spleen in normal experimental animals with the Vena porta constricted with Ameroid rings and liver cirrhosis induced by DMNA. The report also covers 9 patients operated on from 1972 to 1973 with subcutaneous transposition of the spleen. A 77-year-old woman died postoperatively, one patient died after 2 years 4 months, and one had slight bleeding. All the other patients are alive 2-4 years after the operation, with no signs of renewed bleeding. The advantage of subcutaneous transposition of the spleen is that it eliminates hypersplenism."}
{"id": "PMID:1086941", "title": "[New aspects in the treatment of pneumonitis (author's transl)].", "content": "Two groups of patients suffering from pneumonitis received different treatments and were compared retrospectively. The first group (38 patients) was treated with heparin and aprotinin. The overall mortality was 50 percent; of the patients who were artifically ventilated 64 percent died. The second group (60 patients) was treated with heparin, fresh-frozen plasma, nicotinic acid, methylprednisolone, and alpha-receptor blocking agents. In this group the overall mortality was 26.5 percent and the mortality among the patients who were artificially ventilated was 33 percent. These results lead the authors to feel that the treatment of pneumonitis with aprotinin is no longer indicated.", "contents": "[New aspects in the treatment of pneumonitis (author's transl)]. Two groups of patients suffering from pneumonitis received different treatments and were compared retrospectively. The first group (38 patients) was treated with heparin and aprotinin. The overall mortality was 50 percent; of the patients who were artifically ventilated 64 percent died. The second group (60 patients) was treated with heparin, fresh-frozen plasma, nicotinic acid, methylprednisolone, and alpha-receptor blocking agents. In this group the overall mortality was 26.5 percent and the mortality among the patients who were artificially ventilated was 33 percent. These results lead the authors to feel that the treatment of pneumonitis with aprotinin is no longer indicated."}
{"id": "PMID:1086942", "title": "[Postoperative analgesia with continous epidural analgesia and with dolantin (author's transl)].", "content": "Hemodynamic reactions to the discontinuation of epidural analgesia and to the injection of Dolantin were studied in 16 patients. One of every three patients reacted to the postoperative pain with an increase in mean arterial pressure(+30%) and in the mean pressure in the arteria pulmonalis (+40%) associated with an increase in stroke volume (+41%) and cardiac output (+49%). The administration of Dolantin did not influence either pressure measurement. In such cases the administration of antihypertensive drugs (alpha-blocking agents) or the reinstitution of epidural analgesia is neccessary.", "contents": "[Postoperative analgesia with continous epidural analgesia and with dolantin (author's transl)]. Hemodynamic reactions to the discontinuation of epidural analgesia and to the injection of Dolantin were studied in 16 patients. One of every three patients reacted to the postoperative pain with an increase in mean arterial pressure(+30%) and in the mean pressure in the arteria pulmonalis (+40%) associated with an increase in stroke volume (+41%) and cardiac output (+49%). The administration of Dolantin did not influence either pressure measurement. In such cases the administration of antihypertensive drugs (alpha-blocking agents) or the reinstitution of epidural analgesia is neccessary."}
{"id": "PMID:1086943", "title": "[X-ray findings in diverticulitis (author's transl)].", "content": "The frequency of congenital and acquired diverticula is discussed with reference to geographical aspects, their location and the function of the colon in diverticular disease. The X-ray findings in the presence of complications are demonstrated. Radiological differential diagnosis is discussed with special reference to cinematographic analysis of function. The possiblity of the simultaneous appearance of polyposis and carcinoma in diverticular diseases is indicated. A sensitive radiologic diagnostic procedure is important for planning of the optimal treatment.", "contents": "[X-ray findings in diverticulitis (author's transl)]. The frequency of congenital and acquired diverticula is discussed with reference to geographical aspects, their location and the function of the colon in diverticular disease. The X-ray findings in the presence of complications are demonstrated. Radiological differential diagnosis is discussed with special reference to cinematographic analysis of function. The possiblity of the simultaneous appearance of polyposis and carcinoma in diverticular diseases is indicated. A sensitive radiologic diagnostic procedure is important for planning of the optimal treatment."}
{"id": "PMID:1086944", "title": "[Neurophysiological basis of pain and pain therapy (author's transl)].", "content": "Painful stimuli are encoded by specialized nociceptors. In the spinal cord the afferent nerves transmit them at the synapse to dorsal horn neurons, which are considered here as models for neuronal mechanisms in nociception. In particular, inhibitory effects of spinal and of supraspinal origin are shown to modulate the nociceptive messages ascending to the brain. These inhibitions are activated by various methods of electrostimulation (of the peripheral nerves, dorsal columns, electroacupuncture) that are used clinically for the management of pain.", "contents": "[Neurophysiological basis of pain and pain therapy (author's transl)]. Painful stimuli are encoded by specialized nociceptors. In the spinal cord the afferent nerves transmit them at the synapse to dorsal horn neurons, which are considered here as models for neuronal mechanisms in nociception. In particular, inhibitory effects of spinal and of supraspinal origin are shown to modulate the nociceptive messages ascending to the brain. These inhibitions are activated by various methods of electrostimulation (of the peripheral nerves, dorsal columns, electroacupuncture) that are used clinically for the management of pain."}
{"id": "PMID:1086945", "title": "[Methods of pain modulation by electrical stimulation (author's transl)].", "content": "Different methods of modulating pain by electrical stimulation are described: (1) Nondestructive transcutaneous nerve stimulation is recommended for neurogenic pain syndromes prior to other procedures (success rate about 30%); (2) The implantation of electrodes on the dorsal columns yields good effects in 65% after careful selection; (3) First results with implantations in deep brain structures are discussed.", "contents": "[Methods of pain modulation by electrical stimulation (author's transl)]. Different methods of modulating pain by electrical stimulation are described: (1) Nondestructive transcutaneous nerve stimulation is recommended for neurogenic pain syndromes prior to other procedures (success rate about 30%); (2) The implantation of electrodes on the dorsal columns yields good effects in 65% after careful selection; (3) First results with implantations in deep brain structures are discussed."}
{"id": "PMID:1086946", "title": "Neuro-otologic manifestations of sarcoidosis.", "content": "Sarcoidosis is an idiopathic disease which presents in anatomic areas of concern to otorhinolaryngologists. It can cause dysfunction of both auditory and vestibular systems. In patients known previously to have sarcoidosis, this disease should be seriously considered. In patients presenting with otologic disorders and associated facial nerve paralysis or other neuropathies, uveitis, granulomatous meningitis or diabetes insipidus, sarcoidosis should be suspected. An examination of the eyes as well as a chest X-ray is imperative. Sudden and fluctuating neurosensory hearing loss has been reported. There is a great need for the study of temporal bones from sarcoidosis patients.", "contents": "Neuro-otologic manifestations of sarcoidosis. Sarcoidosis is an idiopathic disease which presents in anatomic areas of concern to otorhinolaryngologists. It can cause dysfunction of both auditory and vestibular systems. In patients known previously to have sarcoidosis, this disease should be seriously considered. In patients presenting with otologic disorders and associated facial nerve paralysis or other neuropathies, uveitis, granulomatous meningitis or diabetes insipidus, sarcoidosis should be suspected. An examination of the eyes as well as a chest X-ray is imperative. Sudden and fluctuating neurosensory hearing loss has been reported. There is a great need for the study of temporal bones from sarcoidosis patients."}
{"id": "PMID:1086950", "title": "A problem-orientated outpatient record.", "content": "A problem-orientated record for use in outpatient clinics has been in operation over a period of 2 1/2 years. Its special features include: (i) preprinted stationery bound in appropriate sequence in the conventional hospital record; (ii) clear presentation of an up-to-date list of each patient's past and present diagnoses; (iii) problem-orientated progress notes. This study considers the definition of an illness problem, recording of progress information, adjustments to a problem list in the outpatient setting and recording of minor \"noise\" problems. Experience with the system consisted of 5,000 patient attendances. A sampling of these indicated that there were 3-1 +/- SD 1-5 concurrently treated problems per patient, and there were 9-3 +/- SD 6-6 clinic visits per patient. The system was subjectively assessed by 18 doctors, using a semantic differential technique, with a high frequency of \"yes\" responses to \"saves time\", \"benefits the patient\" and \"benefits the doctor\". This and similar systems may help to resolve the present paradox of the clinical chart becoming decreasingly useful with increasing complexity of illness.", "contents": "A problem-orientated outpatient record. A problem-orientated record for use in outpatient clinics has been in operation over a period of 2 1/2 years. Its special features include: (i) preprinted stationery bound in appropriate sequence in the conventional hospital record; (ii) clear presentation of an up-to-date list of each patient's past and present diagnoses; (iii) problem-orientated progress notes. This study considers the definition of an illness problem, recording of progress information, adjustments to a problem list in the outpatient setting and recording of minor \"noise\" problems. Experience with the system consisted of 5,000 patient attendances. A sampling of these indicated that there were 3-1 +/- SD 1-5 concurrently treated problems per patient, and there were 9-3 +/- SD 6-6 clinic visits per patient. The system was subjectively assessed by 18 doctors, using a semantic differential technique, with a high frequency of \"yes\" responses to \"saves time\", \"benefits the patient\" and \"benefits the doctor\". This and similar systems may help to resolve the present paradox of the clinical chart becoming decreasingly useful with increasing complexity of illness."}
{"id": "PMID:1086952", "title": "The influence of surgery on survival of patients with angina.", "content": "Myocardial revascularization surgery has proved most effective in relieving angina pectoris. Its influence on the survival of patients with coronary atherosclerosis, however, remains a contentious subject and one which may not be finally settled for years. The follow-up results of the first 196 patients to undergo direct coronary artery surgery at the Prince Henry Hospital support those who claim increased longevity as one of its benefits.", "contents": "The influence of surgery on survival of patients with angina. Myocardial revascularization surgery has proved most effective in relieving angina pectoris. Its influence on the survival of patients with coronary atherosclerosis, however, remains a contentious subject and one which may not be finally settled for years. The follow-up results of the first 196 patients to undergo direct coronary artery surgery at the Prince Henry Hospital support those who claim increased longevity as one of its benefits."}
{"id": "PMID:1086953", "title": "Acute upper gastrointestinal haemorrhage with negative barium-meal x-ray findings: follow-up investigation.", "content": "One-hundred and one patients admitted to hospital with acute upper gastrointestinal haemorrhage whose initial barium-meal X-ray examination revealed no abnormality have been followed up 5 to 14 years later. Forty-five were symptom-free, and 8 had dyspepsia, but the barium-meal X-ray findings were still normal. Disease of the lower oesophagus, the stomach or the duodenum was found in 20, 16 of whom had a chronic peptic ulcer and two had gastric carcinoma. There was a poor prognosis for those gastric-ulcer patients whose diagnosis had been missed initially.", "contents": "Acute upper gastrointestinal haemorrhage with negative barium-meal x-ray findings: follow-up investigation. One-hundred and one patients admitted to hospital with acute upper gastrointestinal haemorrhage whose initial barium-meal X-ray examination revealed no abnormality have been followed up 5 to 14 years later. Forty-five were symptom-free, and 8 had dyspepsia, but the barium-meal X-ray findings were still normal. Disease of the lower oesophagus, the stomach or the duodenum was found in 20, 16 of whom had a chronic peptic ulcer and two had gastric carcinoma. There was a poor prognosis for those gastric-ulcer patients whose diagnosis had been missed initially."}
{"id": "PMID:1086987", "title": "Contribution of vasopressor and plasma kininogen changes towards acute adrenaline pulmonary edema in the rat.", "content": "Acute pulmonary edema, evidenced by increased lung/body weight ratios, was evoked in rats within 5 min following the intravenous injection of 16-40 mug/kg of adrenaline. This change was accompanied by a decrease of 40% of circulatory kininogen not due to generalized plasma protein loss. Rats treated 10-20 min prior to adrenaline with 10 mg/kg of acetylsalicylate (Aspirin), 1000 KIU/kg of Kunitz anti-protease (Trasylol), or 10 mg/kg of soybean trysin inhibitor (SBTI), failed to exhibit pulmonary edema or decreased plasma kininogen levels, but were as sensitive as untreated animals to the arterial hypertensive effect of adrenaline. 4.8 mug/kg of carbamylcholine administered together with 40 mug/kg of adrenaline, prevented pulmonary edema. Carbamylcholine did not reduce plasma kininogen consumption by adrenaline, but effectively decreased the raised systolic arterial blood pressure, the increased systolic-diastolic pressure interval as well as the reflex slowing of the heart presented by adrenaline-treated rats. It seems that in the adrenaline-treated rat, pulmonary edema results from the joined action of vasopressor effects leading to hydrostatically forced outflow of vascular fluid, and of kinin release leading to increased peripheral vascular permeability.", "contents": "Contribution of vasopressor and plasma kininogen changes towards acute adrenaline pulmonary edema in the rat. Acute pulmonary edema, evidenced by increased lung/body weight ratios, was evoked in rats within 5 min following the intravenous injection of 16-40 mug/kg of adrenaline. This change was accompanied by a decrease of 40% of circulatory kininogen not due to generalized plasma protein loss. Rats treated 10-20 min prior to adrenaline with 10 mg/kg of acetylsalicylate (Aspirin), 1000 KIU/kg of Kunitz anti-protease (Trasylol), or 10 mg/kg of soybean trysin inhibitor (SBTI), failed to exhibit pulmonary edema or decreased plasma kininogen levels, but were as sensitive as untreated animals to the arterial hypertensive effect of adrenaline. 4.8 mug/kg of carbamylcholine administered together with 40 mug/kg of adrenaline, prevented pulmonary edema. Carbamylcholine did not reduce plasma kininogen consumption by adrenaline, but effectively decreased the raised systolic arterial blood pressure, the increased systolic-diastolic pressure interval as well as the reflex slowing of the heart presented by adrenaline-treated rats. It seems that in the adrenaline-treated rat, pulmonary edema results from the joined action of vasopressor effects leading to hydrostatically forced outflow of vascular fluid, and of kinin release leading to increased peripheral vascular permeability."}
{"id": "PMID:1086988", "title": "L-5-hydroxytryptophan in treatment of several different syndromes in which myoclonus is prominent.", "content": "The serotonin precursor L-5-hydroxytryptophan is useful therapy for patients with posthypoxic intention myoclonus. L-5-hydroxytryptophan plus carbidopa was administered to eight patients with this disorder or other syndromes in which myoclonus is prominent. This treatment (1) decreased the frequency of occurrence and amplitude of intention myoclonus in two patients with posthypoxic intention myoclonus and in one with idiopathic myoclonus, (2) had no effect in one patient with congenital encephalopathy and myoclonus, and (3) increased the frequency of occurrence and amplitude of myoclonus in two patients with lipid storage disease, one with myoclonic epilepsy, and in an additional patient with idiopathic myoclonus. Therefore, L-5-hydroxytryptophan does not effect improvement in all forms of myoclonus; it should be given with caution because it produces a high incidence of side effects. A patient's response to L-5-hydroxytryptophan therapy may be important in a diagnostic classification of myoclonic syndromes based on differences in indoleamine neurotransmitter function.", "contents": "L-5-hydroxytryptophan in treatment of several different syndromes in which myoclonus is prominent. The serotonin precursor L-5-hydroxytryptophan is useful therapy for patients with posthypoxic intention myoclonus. L-5-hydroxytryptophan plus carbidopa was administered to eight patients with this disorder or other syndromes in which myoclonus is prominent. This treatment (1) decreased the frequency of occurrence and amplitude of intention myoclonus in two patients with posthypoxic intention myoclonus and in one with idiopathic myoclonus, (2) had no effect in one patient with congenital encephalopathy and myoclonus, and (3) increased the frequency of occurrence and amplitude of myoclonus in two patients with lipid storage disease, one with myoclonic epilepsy, and in an additional patient with idiopathic myoclonus. Therefore, L-5-hydroxytryptophan does not effect improvement in all forms of myoclonus; it should be given with caution because it produces a high incidence of side effects. A patient's response to L-5-hydroxytryptophan therapy may be important in a diagnostic classification of myoclonic syndromes based on differences in indoleamine neurotransmitter function."}
{"id": "PMID:1086989", "title": "[Alpha 1-antitrypsin in the blood and pleural effusion fluid].", "content": "Alpha-1 antitrypsin levels were determined in 27 pleural effusion liquids classified as transudates and exudates in accordance with the clinical and laboratory data, and in their respective blood samples. Absolute values proved insufficient to distinguish transudates from exudates, though they were capable of signifying and replacing the relative protein value. The relation between liquid: serum alpha-1 AT ratio and the liquid: serum proteins ratio was direct and highly significant. Along with the liquid: serum LAD and liquid: serum proteins ratios, the liquid: serum alpha-1 AT ratio enables transudates and exudates to be differentiated with certainty.", "contents": "[Alpha 1-antitrypsin in the blood and pleural effusion fluid]. Alpha-1 antitrypsin levels were determined in 27 pleural effusion liquids classified as transudates and exudates in accordance with the clinical and laboratory data, and in their respective blood samples. Absolute values proved insufficient to distinguish transudates from exudates, though they were capable of signifying and replacing the relative protein value. The relation between liquid: serum alpha-1 AT ratio and the liquid: serum proteins ratio was direct and highly significant. Along with the liquid: serum LAD and liquid: serum proteins ratios, the liquid: serum alpha-1 AT ratio enables transudates and exudates to be differentiated with certainty."}
{"id": "PMID:1086990", "title": "[Endoscopic study on the gastric tolerance of antalgic-antirheumatic substances:parsalmide].", "content": "The gastric tolerance of parsalmide, a new analgesic, antirheumatic and sedative substance was tested on a group of 50 arthrorheumatic patients with the purpose of assessing gastric damage if any. It was also compared with the tolerance of the better known antitrheumatic drugs in current use. The study was conducted with the endoscopic method, parsalmide being administered in quantities of 600 mg/die per os, broken up into 3 doses, for a period of 20 days. Unlike phenylbutazone, indomethacin and cortisone, parsalmide showed no ulcerogenous effect nor haemorrhage type alterations at gastroduodenal level, at least in the cases treated. Furthermore, the slight surface oedematous gastropathies that appeared during the research were not important enough to demand suspension of the treatment. Endoscopically this picture can be judged of little clinical importance (as with the majority of well known analgesic-antirheumatic drugs) and its incidence came within the mean. A fair tranquillizing action, detectable in the particularly amenable behaviour of the subjects undergoing endoscopy, is also worth reporting.", "contents": "[Endoscopic study on the gastric tolerance of antalgic-antirheumatic substances:parsalmide]. The gastric tolerance of parsalmide, a new analgesic, antirheumatic and sedative substance was tested on a group of 50 arthrorheumatic patients with the purpose of assessing gastric damage if any. It was also compared with the tolerance of the better known antitrheumatic drugs in current use. The study was conducted with the endoscopic method, parsalmide being administered in quantities of 600 mg/die per os, broken up into 3 doses, for a period of 20 days. Unlike phenylbutazone, indomethacin and cortisone, parsalmide showed no ulcerogenous effect nor haemorrhage type alterations at gastroduodenal level, at least in the cases treated. Furthermore, the slight surface oedematous gastropathies that appeared during the research were not important enough to demand suspension of the treatment. Endoscopically this picture can be judged of little clinical importance (as with the majority of well known analgesic-antirheumatic drugs) and its incidence came within the mean. A fair tranquillizing action, detectable in the particularly amenable behaviour of the subjects undergoing endoscopy, is also worth reporting."}
{"id": "PMID:1086992", "title": "Acute renal failure of obstetric origin.", "content": "Acute renal failure of obstetric origin is common among North Indian patients and comprised 72 (22.1%) of 325 patients undergoing dialysis over an 11-year period. Of these, 46 gravidas had developed renal failure following abortion, and 29 cases were due to complications of late pregnancy. The most striking feature of this study was a high incidence of irreversible renal lesions of bilateral diffuse cortical necrosis in early (18.6%) as well as late pregnancy (37.8%). Overall incidence of diffuse cortical necrosis was 25%. In the remainder, acute tubular necrosis was seen in 52 (72.2%), patchy cortical necrosis in 1 (1.4%), and tubular necrosis along with glomerular involvement in 1 patient (1.4%). Pathogenetic factors which contributed to the development of renal failure, either singly or in combination, were loss of blood failure, either singly or in combination, were loss of blood (79.1%), septicemia (31.9%), hypotension due th hemorrhagic and septicemic shock (51.4%), eclamptic toxemia (11.1%), and disseminated intravascular coagulation in 12.5% patients. Infrequent occurrence of disseminated intravascular coagulation in the septic anc eclamptic patients who developed diffuse cortical necrosis was an interesting finding, as was the fact that coagulopathy was more frequently observed in acute tubular necrosis. Late referral, frequent sepsis, and high incidence of bilateral diffuse cortical necrosis contributed significantly to a high mortality (55.3%).", "contents": "Acute renal failure of obstetric origin. Acute renal failure of obstetric origin is common among North Indian patients and comprised 72 (22.1%) of 325 patients undergoing dialysis over an 11-year period. Of these, 46 gravidas had developed renal failure following abortion, and 29 cases were due to complications of late pregnancy. The most striking feature of this study was a high incidence of irreversible renal lesions of bilateral diffuse cortical necrosis in early (18.6%) as well as late pregnancy (37.8%). Overall incidence of diffuse cortical necrosis was 25%. In the remainder, acute tubular necrosis was seen in 52 (72.2%), patchy cortical necrosis in 1 (1.4%), and tubular necrosis along with glomerular involvement in 1 patient (1.4%). Pathogenetic factors which contributed to the development of renal failure, either singly or in combination, were loss of blood failure, either singly or in combination, were loss of blood (79.1%), septicemia (31.9%), hypotension due th hemorrhagic and septicemic shock (51.4%), eclamptic toxemia (11.1%), and disseminated intravascular coagulation in 12.5% patients. Infrequent occurrence of disseminated intravascular coagulation in the septic anc eclamptic patients who developed diffuse cortical necrosis was an interesting finding, as was the fact that coagulopathy was more frequently observed in acute tubular necrosis. Late referral, frequent sepsis, and high incidence of bilateral diffuse cortical necrosis contributed significantly to a high mortality (55.3%)."}
{"id": "PMID:1087001", "title": "Quantitation of circulating T and B lymphocytes in children with whooping cough.", "content": "The numbers of circulating T and B lymphocytes in seven children with whooping cough due to Bordetella pertussis and eight control subjects were determined. All the children with whooping cough had an absolute lymphocytosis (mean 29,142/mm3vs. 5,225/mm3) and by surface marker criteria both T cells and B cells were increased (mean T cells, whooping cough 15,794/mm3 vs. 3,516/mm3 controls; mean B cells, 13,393/mm3 whooping cough vs. 1,706/mm3 controls). However, the ratios of T cells to B cells in the whooping cough (1.4) and control group (1.9) did not differ significantly. This proportional increase in both T and B lymphocytes indicates that whatever mechanism are responsible for lymphocytosis in B. pertussis infection affect both populations in a similar manner.", "contents": "Quantitation of circulating T and B lymphocytes in children with whooping cough. The numbers of circulating T and B lymphocytes in seven children with whooping cough due to Bordetella pertussis and eight control subjects were determined. All the children with whooping cough had an absolute lymphocytosis (mean 29,142/mm3vs. 5,225/mm3) and by surface marker criteria both T cells and B cells were increased (mean T cells, whooping cough 15,794/mm3 vs. 3,516/mm3 controls; mean B cells, 13,393/mm3 whooping cough vs. 1,706/mm3 controls). However, the ratios of T cells to B cells in the whooping cough (1.4) and control group (1.9) did not differ significantly. This proportional increase in both T and B lymphocytes indicates that whatever mechanism are responsible for lymphocytosis in B. pertussis infection affect both populations in a similar manner."}
{"id": "PMID:1087003", "title": "Responses to tilting of fibers of the frog's saccular nerve.", "content": "The activity of single fibers from the saccular nerve has been recorded in isolated head preparations in the frog (Rana esculenta). Nearly all the fibers present an activation or a depression of their activity during the tilting movement in a given direction. This is considered to correspond to the existence of two anatomical populations of haircells in the saccular macula which differ in their functional polarization. The activity of only some units (about 40%) remained increased or decreased when the head was in a tilted position for 4-5 min. This shows that the sacculus is sensitive to the direction of gravity.", "contents": "Responses to tilting of fibers of the frog's saccular nerve. The activity of single fibers from the saccular nerve has been recorded in isolated head preparations in the frog (Rana esculenta). Nearly all the fibers present an activation or a depression of their activity during the tilting movement in a given direction. This is considered to correspond to the existence of two anatomical populations of haircells in the saccular macula which differ in their functional polarization. The activity of only some units (about 40%) remained increased or decreased when the head was in a tilted position for 4-5 min. This shows that the sacculus is sensitive to the direction of gravity."}
{"id": "PMID:1087004", "title": "The theoretical small signal impedance of the frog node, Rana pipiens.", "content": "The small signal impedance of the frog node is calculated for frequencies from 1 Hz to 10,000 Hz and transmembrane potentials from -80 mV to -30 mV, by linearizing the voltage clamp equations of Dodge [7] and Hille [8]. The modulus of the impedance is presented for the total system, and separately for the potassium and sodium systems as a function of frequency and voltage. There is a broad resonance in the total impedance with a voltage-dependent peak frequency. At 22 degrees C, in the range -75 mV to -45 mV, the peak frequencies occur between 50 and 500 Hz. Removing the potassium system leaves a relatively sharp resonance centered around 200 Hz at -45 mV.", "contents": "The theoretical small signal impedance of the frog node, Rana pipiens. The small signal impedance of the frog node is calculated for frequencies from 1 Hz to 10,000 Hz and transmembrane potentials from -80 mV to -30 mV, by linearizing the voltage clamp equations of Dodge [7] and Hille [8]. The modulus of the impedance is presented for the total system, and separately for the potassium and sodium systems as a function of frequency and voltage. There is a broad resonance in the total impedance with a voltage-dependent peak frequency. At 22 degrees C, in the range -75 mV to -45 mV, the peak frequencies occur between 50 and 500 Hz. Removing the potassium system leaves a relatively sharp resonance centered around 200 Hz at -45 mV."}
{"id": "PMID:1087007", "title": "[Radioimmunoassay of antihemophilic factor A].", "content": "A radioimmunological method for the estimation of anti-haemophilic factor A was perfected and studied, making it possible to measure factor VII antigen as distinct from procoagulant factor VIII activity. The method uses a rabbit anti-factor VIII antibody, purified and labelled with I125. The technique of estimation is based upon the differential precipitability of specific factor VIII antigen-antibody complexes, which precipitate with 25 per cent saturated ammonium sulphate and of free antibody which does not precipitate under these conditions. The method is reproducible and sensitive, with the possibility of measuring 0.3 per cent of factor VIII antigen, i.e. 0.003 units/ml. Its specificity is confirmed by the absence of factor VIII antigen in a patient suffering from a severe form of von Willebrand's disease. Using this technique, normal subjects were found to have 96 +/- 31 per cent of factor VIII antigen in comparison with a pool of 25 normal plasmas. Haemophiliacs tested had, as would have been expected, antigen levels close to normal.", "contents": "[Radioimmunoassay of antihemophilic factor A]. A radioimmunological method for the estimation of anti-haemophilic factor A was perfected and studied, making it possible to measure factor VII antigen as distinct from procoagulant factor VIII activity. The method uses a rabbit anti-factor VIII antibody, purified and labelled with I125. The technique of estimation is based upon the differential precipitability of specific factor VIII antigen-antibody complexes, which precipitate with 25 per cent saturated ammonium sulphate and of free antibody which does not precipitate under these conditions. The method is reproducible and sensitive, with the possibility of measuring 0.3 per cent of factor VIII antigen, i.e. 0.003 units/ml. Its specificity is confirmed by the absence of factor VIII antigen in a patient suffering from a severe form of von Willebrand's disease. Using this technique, normal subjects were found to have 96 +/- 31 per cent of factor VIII antigen in comparison with a pool of 25 normal plasmas. Haemophiliacs tested had, as would have been expected, antigen levels close to normal."}
{"id": "PMID:1087012", "title": "Complications of colonic diverticulitis.", "content": "About half of the patients in whom diverticulitis develops have subsequent attacks. Recurrence increases the likelihood both of complications, such as perforation, abscess, fistula, obstruction, and hemorrhage, and of the necessity for surgical intervention. For these reasons, patients who have had two or more attacks of acute diverticulitis should be considered candidates for prophylactic resection.", "contents": "Complications of colonic diverticulitis. About half of the patients in whom diverticulitis develops have subsequent attacks. Recurrence increases the likelihood both of complications, such as perforation, abscess, fistula, obstruction, and hemorrhage, and of the necessity for surgical intervention. For these reasons, patients who have had two or more attacks of acute diverticulitis should be considered candidates for prophylactic resection."}
{"id": "PMID:1087013", "title": "Surgical management of colonic diverticulitis and complicated diverticulosis.", "content": "The aim of surgery in the management of diverticulitis and complicated diverticulosis is to remove the disease process as quickly as possible. This approach reduces the likelihood of complications and often avoids the necessity of multistage procedures. Indications for resection include failure of a first attack to subside, recurrent attacks, and free perforation with spreading peritonitis. Three-stage procedures should be reserved for extremely serious disease. Other complications requiring surgery are fistula, obstruction, and massive and uncontrolled bleeding. Massive bleeding is caused by diffuse colonic diverticulosis and is rarely associated with diverticulitis. Abdominal mass, obstruction, or recurrent episodes of slight bleeding should suggest the possibility of underlying tumor and are compelling indications for early resection.", "contents": "Surgical management of colonic diverticulitis and complicated diverticulosis. The aim of surgery in the management of diverticulitis and complicated diverticulosis is to remove the disease process as quickly as possible. This approach reduces the likelihood of complications and often avoids the necessity of multistage procedures. Indications for resection include failure of a first attack to subside, recurrent attacks, and free perforation with spreading peritonitis. Three-stage procedures should be reserved for extremely serious disease. Other complications requiring surgery are fistula, obstruction, and massive and uncontrolled bleeding. Massive bleeding is caused by diffuse colonic diverticulosis and is rarely associated with diverticulitis. Abdominal mass, obstruction, or recurrent episodes of slight bleeding should suggest the possibility of underlying tumor and are compelling indications for early resection."}
{"id": "PMID:1087014", "title": "Rheumatic diseases. 2. Therapeutic considerations.", "content": "In the management of rheumatic diseases, the use of corticosteroids should be reserved for active arthritis. Phenylbutazone (Butazolidin) is probably the drug of choice for acute gout and is also effective in ankylosing spondylitis, Reiter's syndrome, and psoriatic arthritis. Indomethacin (Indocin) also is useful in these conditions. Ibuprofen (Motrin) is only slightly more efficacious than aspirin. Aspirin is still the preferred treatment for rheumatoid arthritis and should be tried before ibuprofen. Osteoarthritis of the cervical or lumbar spine calls for a full program of physical therapy. Experimental procedures for total replacement of joints other than hip and knee show promise.", "contents": "Rheumatic diseases. 2. Therapeutic considerations. In the management of rheumatic diseases, the use of corticosteroids should be reserved for active arthritis. Phenylbutazone (Butazolidin) is probably the drug of choice for acute gout and is also effective in ankylosing spondylitis, Reiter's syndrome, and psoriatic arthritis. Indomethacin (Indocin) also is useful in these conditions. Ibuprofen (Motrin) is only slightly more efficacious than aspirin. Aspirin is still the preferred treatment for rheumatoid arthritis and should be tried before ibuprofen. Osteoarthritis of the cervical or lumbar spine calls for a full program of physical therapy. Experimental procedures for total replacement of joints other than hip and knee show promise."}
{"id": "PMID:1087015", "title": "Radiology of colonic diverticular disease.", "content": "Nonsurgical diagnosis of colonic diverticulosis and most complications of diverticular disease is best established by barium enema study. Multiple diverticula can produce narrowing and shortening of the colon that is unrelated to any complication occurring in the diverticula. The diagnosis of diverticulitis is made radiologically be demonstrating the effects on the lumen of the bowel of the pathologic changes visible on gross inspection, eg, local abscess, intramural dissection, fistula, obstruction. Frequently the radiologic findings are subtle.", "contents": "Radiology of colonic diverticular disease. Nonsurgical diagnosis of colonic diverticulosis and most complications of diverticular disease is best established by barium enema study. Multiple diverticula can produce narrowing and shortening of the colon that is unrelated to any complication occurring in the diverticula. The diagnosis of diverticulitis is made radiologically be demonstrating the effects on the lumen of the bowel of the pathologic changes visible on gross inspection, eg, local abscess, intramural dissection, fistula, obstruction. Frequently the radiologic findings are subtle."}
{"id": "PMID:1087016", "title": "The use of antithymocyte globulin in myasthenia gravis.", "content": "Antithymocyte globulin (ATG) has been used to treat three female patients with myastenia gravis. No other immunosuppressive agent was used. All three cases responded favourably to ATG therapy. This suggest that further work should be undertaken in this field though the condition itself is extremely variable and this study was not controlled.", "contents": "The use of antithymocyte globulin in myasthenia gravis. Antithymocyte globulin (ATG) has been used to treat three female patients with myastenia gravis. No other immunosuppressive agent was used. All three cases responded favourably to ATG therapy. This suggest that further work should be undertaken in this field though the condition itself is extremely variable and this study was not controlled."}
{"id": "PMID:1087017", "title": "Antithymocyte antiserum therapy in myasthenia gravis.", "content": "The therapeutic effect of goat anti-human thymocyte antiserum globulin (ATG) was assessed in 10 patients with myasthenia gravis. Prolonged, low dose therapy was used with 1-0--1-6 grams protein administered intramuscularly over a 28-73 day period. Varying degrees of therapeutic response were noted in 8 patients. Fllow-up examinations for over 5 years have been maintained and relapse rates determined. The clinical criteria for aptients selection and characteristics of therapeutic response are presented.", "contents": "Antithymocyte antiserum therapy in myasthenia gravis. The therapeutic effect of goat anti-human thymocyte antiserum globulin (ATG) was assessed in 10 patients with myasthenia gravis. Prolonged, low dose therapy was used with 1-0--1-6 grams protein administered intramuscularly over a 28-73 day period. Varying degrees of therapeutic response were noted in 8 patients. Fllow-up examinations for over 5 years have been maintained and relapse rates determined. The clinical criteria for aptients selection and characteristics of therapeutic response are presented."}
{"id": "PMID:1087018", "title": "Human anti-T-cell cytotoxic sera as an approach to dissection of immunological systems.", "content": "The initial work on the analysis and differentiation of human lymphoid cells is described. Cells from various lymphoid organs and blood were used as the antigen to prepare various antisera. These antisera were then used to define the lymphocyte populations of patients with primary and secondary immunodefiency. They are also being used to begin the analysis of the differentiation of the T-lymphocyte.", "contents": "Human anti-T-cell cytotoxic sera as an approach to dissection of immunological systems. The initial work on the analysis and differentiation of human lymphoid cells is described. Cells from various lymphoid organs and blood were used as the antigen to prepare various antisera. These antisera were then used to define the lymphocyte populations of patients with primary and secondary immunodefiency. They are also being used to begin the analysis of the differentiation of the T-lymphocyte."}
{"id": "PMID:1087019", "title": "Antilymphocyte antibodies:an approach to dissecting the heterogeneity of the immune system.", "content": "Antilymphocyte antibodies can be used to dissect the lymphocyte populations and subpopulations. In the mouse, at least seven gene loci govern the expression of allo-, hetero-and autoantigens at the surface of T-lymphocytes. The antisera, especially the alloantisera, permit definition of the differentiative state of cells and discriminate between subsets of T-cells with different functions. Less is known about human T-lymphocyte differentiation antigens and their genetic determinism. However, heterologous antisera specific for human T-cells have been prepared and their use has been of help in studying this cell population. The antigens recognized by such antisera are present at a higher concentration on thymocytes than on peripheral blood T-lymphocytes. They appear at the surface of bone marrow T-cell precursors after a short incubation with thymic factors. Such markers of T-lymphocytes in various stages of differentiation and the study of in vitro differentiation from precursor cells have already proven to be valuable tools for clinical investigation, especially in the realm of immunodeficiency diseases.", "contents": "Antilymphocyte antibodies:an approach to dissecting the heterogeneity of the immune system. Antilymphocyte antibodies can be used to dissect the lymphocyte populations and subpopulations. In the mouse, at least seven gene loci govern the expression of allo-, hetero-and autoantigens at the surface of T-lymphocytes. The antisera, especially the alloantisera, permit definition of the differentiative state of cells and discriminate between subsets of T-cells with different functions. Less is known about human T-lymphocyte differentiation antigens and their genetic determinism. However, heterologous antisera specific for human T-cells have been prepared and their use has been of help in studying this cell population. The antigens recognized by such antisera are present at a higher concentration on thymocytes than on peripheral blood T-lymphocytes. They appear at the surface of bone marrow T-cell precursors after a short incubation with thymic factors. Such markers of T-lymphocytes in various stages of differentiation and the study of in vitro differentiation from precursor cells have already proven to be valuable tools for clinical investigation, especially in the realm of immunodeficiency diseases."}
{"id": "PMID:1087023", "title": "Sea anemone toxin:a tool to study molecular mechanisms of nerve conduction and excitation-secretion coupling.", "content": "The effects of polypeptide neurotoxin from Anemonia sulcata on nerve conduction in crayfish giant axons and on frog myelinated fibers have been analyzed. The main features of toxin action are the following: (i) the toxin acts at very low doses and its action is apparently irreversible. (ii) The toxin selectively affects the closing (inactivation) of the Na+ channel by slowing it down considerably; it does not alter the opening mechanism of the Na+ channel or the steady-state potassium conductance. (iii) The tetrodotoxin-receptor association is unaffected by previous treatment of the axonal membrane with the sea anemone toxin. (iv) Conversely, the sea anemone toxin can only associate with the membrane when the Na+ channel is open for Na+; it does not bind when the channel is previously blocked by tetrodotoxin. (v) Besides its effect on the action potential, the sea anemone toxin displays a veratridine-type depolarizing action at low Ca2+ concentration which can be suppressed by tetrodotoxin. The sea anemone toxin greatly stimulates the release of gamma-[3H]aminobutyric acid from neurotransmitter-loaded rat brain synaptosomes. The apparent dissociation constant of the neurotoxin-receptor complex in this system is 20 nM. The sea anemone toxin effect is antagonized by tetrodotoxin.", "contents": "Sea anemone toxin:a tool to study molecular mechanisms of nerve conduction and excitation-secretion coupling. The effects of polypeptide neurotoxin from Anemonia sulcata on nerve conduction in crayfish giant axons and on frog myelinated fibers have been analyzed. The main features of toxin action are the following: (i) the toxin acts at very low doses and its action is apparently irreversible. (ii) The toxin selectively affects the closing (inactivation) of the Na+ channel by slowing it down considerably; it does not alter the opening mechanism of the Na+ channel or the steady-state potassium conductance. (iii) The tetrodotoxin-receptor association is unaffected by previous treatment of the axonal membrane with the sea anemone toxin. (iv) Conversely, the sea anemone toxin can only associate with the membrane when the Na+ channel is open for Na+; it does not bind when the channel is previously blocked by tetrodotoxin. (v) Besides its effect on the action potential, the sea anemone toxin displays a veratridine-type depolarizing action at low Ca2+ concentration which can be suppressed by tetrodotoxin. The sea anemone toxin greatly stimulates the release of gamma-[3H]aminobutyric acid from neurotransmitter-loaded rat brain synaptosomes. The apparent dissociation constant of the neurotoxin-receptor complex in this system is 20 nM. The sea anemone toxin effect is antagonized by tetrodotoxin."}
{"id": "PMID:1087024", "title": "Role of antigenic structure in cell to cell cooperation.", "content": "Two synthetic polypeptides which differ only in the order of amino acids in their NH2-terminal side chains, namely, (Tyr-Tyr-Glu-Glu)-poly(DLAla)- -poly(LLys) and (Tyr-Glu-Try-Glu)-poly(DLAla)- -poly(LLys), were found to be under different genetic control. By three different in vivo systems for thymus-derived cell depletion, it was demonstrated that (Tyr-Tyr-Glu-Glu)-poly(DLAla)- -poly(LLys), which represents the random poly(Tyr,Glu)-poly(DLAla)- -poly(Lys) in the pattern of immune responses and in the quality of antibodies they elicit, is thymus-dependent whereas (Tyr-Glu-Tyr-Glu)-poly(DLAla)-poly(LLys) does not require thymus-derived cell help for efficient antibody production. Therefore, the two ordered polypeptides which are similar chemically differ in parameters, not yet determined, which affect their capability to trigger bone marrow-derived cells.", "contents": "Role of antigenic structure in cell to cell cooperation. Two synthetic polypeptides which differ only in the order of amino acids in their NH2-terminal side chains, namely, (Tyr-Tyr-Glu-Glu)-poly(DLAla)- -poly(LLys) and (Tyr-Glu-Try-Glu)-poly(DLAla)- -poly(LLys), were found to be under different genetic control. By three different in vivo systems for thymus-derived cell depletion, it was demonstrated that (Tyr-Tyr-Glu-Glu)-poly(DLAla)- -poly(LLys), which represents the random poly(Tyr,Glu)-poly(DLAla)- -poly(Lys) in the pattern of immune responses and in the quality of antibodies they elicit, is thymus-dependent whereas (Tyr-Glu-Tyr-Glu)-poly(DLAla)-poly(LLys) does not require thymus-derived cell help for efficient antibody production. Therefore, the two ordered polypeptides which are similar chemically differ in parameters, not yet determined, which affect their capability to trigger bone marrow-derived cells."}
{"id": "PMID:1087028", "title": "Effect of serotoninergic drugs on positive and negative reinforcing systems in cats.", "content": "Administration of tryptophan and 5-hydroxytryptophan produced inhibition of self-stimulation through hypothalamic electrodes which was accompanied by drowsiness and decrease of motor activity. In cats pretreated with Ro 4-4602, an inhibitor of peripheral decarboxylase, both drugs did not produce drowsiness and markedly activated self-stimulation. Both drugs prolonged the latency of the escape reaction and inhibited the punishment reaction. The data suggest an activating effect of serotonin on the system of reward and an inhibitory effect on the system of punishment.", "contents": "Effect of serotoninergic drugs on positive and negative reinforcing systems in cats. Administration of tryptophan and 5-hydroxytryptophan produced inhibition of self-stimulation through hypothalamic electrodes which was accompanied by drowsiness and decrease of motor activity. In cats pretreated with Ro 4-4602, an inhibitor of peripheral decarboxylase, both drugs did not produce drowsiness and markedly activated self-stimulation. Both drugs prolonged the latency of the escape reaction and inhibited the punishment reaction. The data suggest an activating effect of serotonin on the system of reward and an inhibitory effect on the system of punishment."}
{"id": "PMID:1087029", "title": "Kasabach-kasabach-.", "content": "Giant haemangiomas combined with a haemostasis disorder of the Kasabach-Meritt type are rare and even more rare are the problems posed during labour when vulvo-vagino-uterine sites are involved. Two cases are reported. After summary of the clinical and biochemical studies, the authors report attempts to improve the haemostasis disorder, first with heparin and then with antifibrinolytic preparations, and to improve the angiomas with antifibrinolytic preparations in the hope of stimulating intrasacular thrombosis. In particular, the obstetric and surgical problems are discussed in the context of labour. In one case the outcome of labour was successful only moderate transfusion being required, and in the other the outcome was fatal despite the use of about 100 bottles of blood in the first 24 hours. In both cases the infants were normal.", "contents": "Kasabach-kasabach-. Giant haemangiomas combined with a haemostasis disorder of the Kasabach-Meritt type are rare and even more rare are the problems posed during labour when vulvo-vagino-uterine sites are involved. Two cases are reported. After summary of the clinical and biochemical studies, the authors report attempts to improve the haemostasis disorder, first with heparin and then with antifibrinolytic preparations, and to improve the angiomas with antifibrinolytic preparations in the hope of stimulating intrasacular thrombosis. In particular, the obstetric and surgical problems are discussed in the context of labour. In one case the outcome of labour was successful only moderate transfusion being required, and in the other the outcome was fatal despite the use of about 100 bottles of blood in the first 24 hours. In both cases the infants were normal."}
{"id": "PMID:1087035", "title": "Selective arterial embolization in duodenal bleeding.", "content": "Severe, unremitting bleeding of the upper gastrointestinal tract could be controlled by Gelfoam embolization of the posterior pancreaticoduodenal artery. The technique and indications of the method are presented.", "contents": "Selective arterial embolization in duodenal bleeding. Severe, unremitting bleeding of the upper gastrointestinal tract could be controlled by Gelfoam embolization of the posterior pancreaticoduodenal artery. The technique and indications of the method are presented."}
{"id": "PMID:1087036", "title": "Effect of apomorphine plus 5-hydroxytryptophan on plasma prolactin levels in male rats.", "content": "The relative potency of dopaminergic inhibition and serotonergic stimulation of prolactin secretion in male rats was compared. 5-Hydroxytryptophan (5-HTP), 100 mg/kg, i.p., the precursor of serotonin, produced a 6-11 fold increase in plasma prolactin. Apomorphine, a dopamine agonist, at doses of 1-10 mg/kg, i.p., had no significant effect on plasma prolactin. However, when apomorphine was given with or before 5-HTP, it nearly completely blocked the increase in prolactin produced by 5-htp. these results indicate that inhibition of prolactin secretion by dopaminergic stimulation can overcome the prolactin releasing effect of serotonin. The results are consistent with the hypothesis that prolactin secretion is ordinarily under a weak serotonergic stimulation and a profound dopaminergic inhibition. It is also possible that apomorphine affects plasma prolactin levels by increasing prolactin clearance.", "contents": "Effect of apomorphine plus 5-hydroxytryptophan on plasma prolactin levels in male rats. The relative potency of dopaminergic inhibition and serotonergic stimulation of prolactin secretion in male rats was compared. 5-Hydroxytryptophan (5-HTP), 100 mg/kg, i.p., the precursor of serotonin, produced a 6-11 fold increase in plasma prolactin. Apomorphine, a dopamine agonist, at doses of 1-10 mg/kg, i.p., had no significant effect on plasma prolactin. However, when apomorphine was given with or before 5-HTP, it nearly completely blocked the increase in prolactin produced by 5-htp. these results indicate that inhibition of prolactin secretion by dopaminergic stimulation can overcome the prolactin releasing effect of serotonin. The results are consistent with the hypothesis that prolactin secretion is ordinarily under a weak serotonergic stimulation and a profound dopaminergic inhibition. It is also possible that apomorphine affects plasma prolactin levels by increasing prolactin clearance."}
{"id": "PMID:1087041", "title": "Positive inotropic action of Solanaceae glycoalkaloids.", "content": "Six glycoalkaloids and one aglycone were tested for cardiotonic activities and compared with K-strophanthoside by use of the isolated frog heart. The decreasing order of potency was as follows: K-strophanthoside greater than tomatine greater than alpha-chaconine=alpha-solanine greater than demissine=commersonine greater than beta-chaconine greater than solanidine. Cardiotonic activity was directly related to the number of sugars in the molecule in which the glycoalkaloids had a common aglycone. On the other hand, glycoalkaloids with dissimilar aglycones, but with identical sugars, such as tomatine and demissine, differed significantly with respect to cardiotonic potency. Apparently, therefore, the cardiotonic activity of glycoalkaloids on the frog heart is determined by the nature of the aglycone and the number of sugars and not by the kinds of sugars or their stereochemical configuration.", "contents": "Positive inotropic action of Solanaceae glycoalkaloids. Six glycoalkaloids and one aglycone were tested for cardiotonic activities and compared with K-strophanthoside by use of the isolated frog heart. The decreasing order of potency was as follows: K-strophanthoside greater than tomatine greater than alpha-chaconine=alpha-solanine greater than demissine=commersonine greater than beta-chaconine greater than solanidine. Cardiotonic activity was directly related to the number of sugars in the molecule in which the glycoalkaloids had a common aglycone. On the other hand, glycoalkaloids with dissimilar aglycones, but with identical sugars, such as tomatine and demissine, differed significantly with respect to cardiotonic potency. Apparently, therefore, the cardiotonic activity of glycoalkaloids on the frog heart is determined by the nature of the aglycone and the number of sugars and not by the kinds of sugars or their stereochemical configuration."}
{"id": "PMID:1087042", "title": "On the mechanism of activation of the hypothalamo--pituitary--adrenal reaction to changes in the environment (the 'alarm reaction').", "content": "The amount of circulating noradrenaline increases considerably in parallel with an improvement in cardiovascular haemodynamics in adrenalectomized rabbits after intravenously injected hydrocortisone. In view of this finding, that as well as a constant regulation with negative retroaction of the corticotrophic-releasing factor--adrenocorticotrophic hormone (ACTH)--cortisol system, there exists an externally controlled servomechanism from nervous system activity related to the environment. Hydrocortisone favours passive avoidance, extinction and facilitates the activity of the cholinergic inhibiting system; ACTH facilitates active avoidance and catecholaminergic activation. It has already been demonstrated that stimulation of the inhibiting system or inhibition of the activating system released noradrenaline from the peripheral nerves of an adrenalectomized animal. It therefore seems that the early release of ACTH after aggression is capable of favouring locomotor activity in 'flight or fight'. If the latter is effective, there is a return to the previous behavioural and endocrine balance. On the other hand, if 'flight or fight' should be ineffective, there arises a vicious circle with positive retroaction, because secondary secretion of glucocorticoids will maintain inhibition and considerable activity in the noradrenergic system. The significance of the alarm reaction and the secondary release of the mineralocorticoids are discussed as well as the central mechanism of allegedly psychosomatic, illness. Aggression, as well as the lesion it induces, causes a reaction from the organism subjected to aggression. This is a non-specific reaction to which Selye (1936) has drawn attention. This reaction sets the central nervous system in action, which activates the vegetative nervous system and the endocrine system through the hypothalamus and the pituitary gland. In the vegetative system and the vasomotor changes related to it, the alarm syndrome has to be understood as a way to induce flight or fight, which allows the organism to move away from the aggressor. When this behaviour is impossible or ineffective the vasomotor reaction is threatened and it is depressed or inhibited (Laborit, 1952). Resuscitation techniques involving central or peripheral inhibitors, such as lytic cocktails, neuroplegy or neuroleptanalgesia, derive from this concept. The neuro--endocrine significance of the alarm reaction is still not understood and resuscitation techniques are quite empirical in relation to it.", "contents": "On the mechanism of activation of the hypothalamo--pituitary--adrenal reaction to changes in the environment (the 'alarm reaction'). The amount of circulating noradrenaline increases considerably in parallel with an improvement in cardiovascular haemodynamics in adrenalectomized rabbits after intravenously injected hydrocortisone. In view of this finding, that as well as a constant regulation with negative retroaction of the corticotrophic-releasing factor--adrenocorticotrophic hormone (ACTH)--cortisol system, there exists an externally controlled servomechanism from nervous system activity related to the environment. Hydrocortisone favours passive avoidance, extinction and facilitates the activity of the cholinergic inhibiting system; ACTH facilitates active avoidance and catecholaminergic activation. It has already been demonstrated that stimulation of the inhibiting system or inhibition of the activating system released noradrenaline from the peripheral nerves of an adrenalectomized animal. It therefore seems that the early release of ACTH after aggression is capable of favouring locomotor activity in 'flight or fight'. If the latter is effective, there is a return to the previous behavioural and endocrine balance. On the other hand, if 'flight or fight' should be ineffective, there arises a vicious circle with positive retroaction, because secondary secretion of glucocorticoids will maintain inhibition and considerable activity in the noradrenergic system. The significance of the alarm reaction and the secondary release of the mineralocorticoids are discussed as well as the central mechanism of allegedly psychosomatic, illness. Aggression, as well as the lesion it induces, causes a reaction from the organism subjected to aggression. This is a non-specific reaction to which Selye (1936) has drawn attention. This reaction sets the central nervous system in action, which activates the vegetative nervous system and the endocrine system through the hypothalamus and the pituitary gland. In the vegetative system and the vasomotor changes related to it, the alarm syndrome has to be understood as a way to induce flight or fight, which allows the organism to move away from the aggressor. When this behaviour is impossible or ineffective the vasomotor reaction is threatened and it is depressed or inhibited (Laborit, 1952). Resuscitation techniques involving central or peripheral inhibitors, such as lytic cocktails, neuroplegy or neuroleptanalgesia, derive from this concept. The neuro--endocrine significance of the alarm reaction is still not understood and resuscitation techniques are quite empirical in relation to it."}
{"id": "PMID:1087044", "title": "Abnormal lymphocyte populations in pure red cell aplasia.", "content": "Studies of blood lymphocytes from 4 patients with pure red cell aplasia were performed with lymphocyte surface markers, and with various in vitro tests for lymphocyte functions. Pathologically low B-lymphocyte values were found. In contrast, no marked deviation from normals were seen for T-lymphocytes and Fc-receptor-bearing lymphocytes thought largely to represent non-B, non-T-lymphocytes. In 3 patients normal lymphocyte transformation was found with unspecific and specific mitogens, while the DNA-synthesis induced by unspecific mitogens was subnormal in the fourth patients. The lymphocyte-mediated PHA-induced cytotoxicity against target cells in vitro was subnormal in 2 patients, while no depression was seen in antibody-dependent cytotoxicity mediated in vitro by lymphocytes (K-cells). It is concluded that considerable immunological abnormalities are associated with pure red cell aplasia, and the possible significance of this is discussed.", "contents": "Abnormal lymphocyte populations in pure red cell aplasia. Studies of blood lymphocytes from 4 patients with pure red cell aplasia were performed with lymphocyte surface markers, and with various in vitro tests for lymphocyte functions. Pathologically low B-lymphocyte values were found. In contrast, no marked deviation from normals were seen for T-lymphocytes and Fc-receptor-bearing lymphocytes thought largely to represent non-B, non-T-lymphocytes. In 3 patients normal lymphocyte transformation was found with unspecific and specific mitogens, while the DNA-synthesis induced by unspecific mitogens was subnormal in the fourth patients. The lymphocyte-mediated PHA-induced cytotoxicity against target cells in vitro was subnormal in 2 patients, while no depression was seen in antibody-dependent cytotoxicity mediated in vitro by lymphocytes (K-cells). It is concluded that considerable immunological abnormalities are associated with pure red cell aplasia, and the possible significance of this is discussed."}
{"id": "PMID:1087045", "title": "Effects of anticonvulsant drugs on the synthesis of DNA and protein by human bone marrow cells in vitro.", "content": "Suspensions of human bone marrow cells were incubated with various concentrations of phenobarbitone or phenytoin sodium for 2 h, and the effects of this incubation on the subsequent incorporation of 3H-thymidine and3H-leucine into DNA and protein, respectively, were studied. Both drugs caused a depression of 3H-thymidine incorporation and this phenomenon was not prevented by the addition of 100 mug of pteroyl-glutamic acid, folinic acid or 5-methyltetrahydrofolate per ml of marrow culture. The lowest concentration of drug which caused a statistically significant depression of 3H-thymidine incorporation was 200 mug per ml for phenobarbitone and 50 mug per ml for phenytoin sodium. Both phenobarbitone and phenytoin sodium also caused an increase in the incorporation of 3H-leucine at concentrations of 50 and 20 mug per ml, respectively, suggesting the possibility that a stimulation of protein synthesis within erythropoietic cells may play an important role in the development of anticonvulsant-induced macrocytosis.", "contents": "Effects of anticonvulsant drugs on the synthesis of DNA and protein by human bone marrow cells in vitro. Suspensions of human bone marrow cells were incubated with various concentrations of phenobarbitone or phenytoin sodium for 2 h, and the effects of this incubation on the subsequent incorporation of 3H-thymidine and3H-leucine into DNA and protein, respectively, were studied. Both drugs caused a depression of 3H-thymidine incorporation and this phenomenon was not prevented by the addition of 100 mug of pteroyl-glutamic acid, folinic acid or 5-methyltetrahydrofolate per ml of marrow culture. The lowest concentration of drug which caused a statistically significant depression of 3H-thymidine incorporation was 200 mug per ml for phenobarbitone and 50 mug per ml for phenytoin sodium. Both phenobarbitone and phenytoin sodium also caused an increase in the incorporation of 3H-leucine at concentrations of 50 and 20 mug per ml, respectively, suggesting the possibility that a stimulation of protein synthesis within erythropoietic cells may play an important role in the development of anticonvulsant-induced macrocytosis."}
{"id": "PMID:1087047", "title": "[The morphological and functional integrity of cryo-preserved leukocytes].", "content": "Human peripheral blood lymphocytes were frozen by a simplified method which is described. Light and electron microscopic investigations of the frozen-thawed cells showed no significant morphological changes compared to the fresh cells; comparison of E-rosette formation and nonspecific acid esterase activity before and after cryoconservation afford evidence of a possible slight change in the percent relationship of T- and B-cells, whereas thymidine uptake values within normal range after stimulation with PHA and homologous cells demonstrate the functional integrity of the lymphocytes.", "contents": "[The morphological and functional integrity of cryo-preserved leukocytes]. Human peripheral blood lymphocytes were frozen by a simplified method which is described. Light and electron microscopic investigations of the frozen-thawed cells showed no significant morphological changes compared to the fresh cells; comparison of E-rosette formation and nonspecific acid esterase activity before and after cryoconservation afford evidence of a possible slight change in the percent relationship of T- and B-cells, whereas thymidine uptake values within normal range after stimulation with PHA and homologous cells demonstrate the functional integrity of the lymphocytes."}
{"id": "PMID:1087050", "title": "Combined aortoiliac and femoropopliteal occlusive disease.", "content": "In a series of 140 patients operated upon for aortoiliac occlusive disease over 11 1/2 years, 81 had concomitant occlusion of the superficial femoral artery. These 81 patients with combined segment disease were studied with particular emphasis on the relief of presenting symptoms by an aortic bypass procedure alone. Fifty-three patients underwent only aortic bypass and could be fully evaluated postoperatively. Ninety-six per cent presenting with rest pain or ischemic changes obtained complete relief of these sympotms. All 53 patients presented with claudication, and 88.7 per cent either had complete relief or improved to the point that no further theapy was needed. The results of this study confirm the efficacy of restoration of proximal flow to the profunda femoris arteries as the primary therapeutic goal in patients with combined segment disease.", "contents": "Combined aortoiliac and femoropopliteal occlusive disease. In a series of 140 patients operated upon for aortoiliac occlusive disease over 11 1/2 years, 81 had concomitant occlusion of the superficial femoral artery. These 81 patients with combined segment disease were studied with particular emphasis on the relief of presenting symptoms by an aortic bypass procedure alone. Fifty-three patients underwent only aortic bypass and could be fully evaluated postoperatively. Ninety-six per cent presenting with rest pain or ischemic changes obtained complete relief of these sympotms. All 53 patients presented with claudication, and 88.7 per cent either had complete relief or improved to the point that no further theapy was needed. The results of this study confirm the efficacy of restoration of proximal flow to the profunda femoris arteries as the primary therapeutic goal in patients with combined segment disease."}
{"id": "PMID:1087051", "title": "Left main stem coronary artery disease. Retrospective review of 26 patients treated surgically or medically.", "content": "The clinical, angiographic, exercise testing, operative, and follow-up data of 26 patients found at angiography to have left main stem coronary artery stenosis, defined as a reduction in the lumen diameter of 50% or more, are reviewed. There was a high incidence of significant proximal stenosis in the branches of the left main stem. No clinical features were found to distinguish patients with left main stem stenosis. All patients were considered for saphenous vein bypass grafting, selection being based upon the severity of symptoms, left ventricular function, and suitability of the coronary vessels for grafting. Two patients died within 24 hours of coronary angiography. Nine patients were operated on with no mortality. There has been one late cardiac death during a mean follow-up time of 13 months. All patients were symptomatically improved with a significant (P less than 0-01) increase in exercise ability postoperatively. Fifteen patients were not operated on. Six of these patients were regarded as operable but surgery was deferred; five have died at a mean time of 7-2 months. Five of the nine patients regarded as inoperable have died at a mean follow-up time of 14-8 months. The five non-surgical survivors remain symptomatic with no significant change in exercise ability. Recently reported surgical and medical series of patients with left main stem stenosis are reviewed.", "contents": "Left main stem coronary artery disease. Retrospective review of 26 patients treated surgically or medically. The clinical, angiographic, exercise testing, operative, and follow-up data of 26 patients found at angiography to have left main stem coronary artery stenosis, defined as a reduction in the lumen diameter of 50% or more, are reviewed. There was a high incidence of significant proximal stenosis in the branches of the left main stem. No clinical features were found to distinguish patients with left main stem stenosis. All patients were considered for saphenous vein bypass grafting, selection being based upon the severity of symptoms, left ventricular function, and suitability of the coronary vessels for grafting. Two patients died within 24 hours of coronary angiography. Nine patients were operated on with no mortality. There has been one late cardiac death during a mean follow-up time of 13 months. All patients were symptomatically improved with a significant (P less than 0-01) increase in exercise ability postoperatively. Fifteen patients were not operated on. Six of these patients were regarded as operable but surgery was deferred; five have died at a mean time of 7-2 months. Five of the nine patients regarded as inoperable have died at a mean follow-up time of 14-8 months. The five non-surgical survivors remain symptomatic with no significant change in exercise ability. Recently reported surgical and medical series of patients with left main stem stenosis are reviewed."}
{"id": "PMID:1087061", "title": "[Hemorrhage in erosive gastritis].", "content": "The authors have examined 381 patients admitted with acute hemorrhage from the upper portion of the gastrointestinal tract. In 58 patients erosive gastritis was found by means of urgent gastroduodenoscopy. The clinical picture of this severe affection, the possibilities of diagnosis and the policy of treatment are described. Electron-microscopic studies of the gastric mucosa in erosive gastritis have evidenced different genesis of the erosions.", "contents": "[Hemorrhage in erosive gastritis]. The authors have examined 381 patients admitted with acute hemorrhage from the upper portion of the gastrointestinal tract. In 58 patients erosive gastritis was found by means of urgent gastroduodenoscopy. The clinical picture of this severe affection, the possibilities of diagnosis and the policy of treatment are described. Electron-microscopic studies of the gastric mucosa in erosive gastritis have evidenced different genesis of the erosions."}
{"id": "PMID:1087062", "title": "[Diverticulosis of the large intestine].", "content": "Under observation were 47 patients with colonic diverticula. The clinical classification of diverticulosis of the colon is suggested. The principal method of treatment in patients with uncomplicated forms of the diverticulosis should be conservative. In profuse hemorrhage and intestinal obstruction as well as in perforation of the diverticulum, that would not respond to conservative therapy, surgery is indicated.", "contents": "[Diverticulosis of the large intestine]. Under observation were 47 patients with colonic diverticula. The clinical classification of diverticulosis of the colon is suggested. The principal method of treatment in patients with uncomplicated forms of the diverticulosis should be conservative. In profuse hemorrhage and intestinal obstruction as well as in perforation of the diverticulum, that would not respond to conservative therapy, surgery is indicated."}
{"id": "PMID:1087071", "title": "[Maternal mortality in Austria from a clinical point of view].", "content": "The maternal mortality rate of 22.4 per 100,000 live births in 1973 is the lowest recorded in Austria. The figures for Austria are compared with the data from other European countries with similar health systems. Austria reflects the overall trend of a decreasing maternal mortality rate. 10 cases of maternal death recorded per 16,447 live births in the 2nd Department of Obstetrics, University of Vienna are presented and discussed with respect to the clinical diagnosis and compared with the main causes of maternal death in the national and international literature. As in other countries, haemorrhage in 3 out of the 10 cases ranks first, whilst in Austria generally, toxaemia has been the most common cause of death in recent years. The main cause of the bleeding is undetected rupture of the uterus, which is frequently concealed behind the clinical diagnosis of circulatory failure with cardiac arrest. 2/3 of the observed cases in the present series are considered to have been avoidable. It is recommended that in future every single reported case of maternal death in Austria is analyzed and the cause of death and the degree of avoidability discussed as is done in England.", "contents": "[Maternal mortality in Austria from a clinical point of view]. The maternal mortality rate of 22.4 per 100,000 live births in 1973 is the lowest recorded in Austria. The figures for Austria are compared with the data from other European countries with similar health systems. Austria reflects the overall trend of a decreasing maternal mortality rate. 10 cases of maternal death recorded per 16,447 live births in the 2nd Department of Obstetrics, University of Vienna are presented and discussed with respect to the clinical diagnosis and compared with the main causes of maternal death in the national and international literature. As in other countries, haemorrhage in 3 out of the 10 cases ranks first, whilst in Austria generally, toxaemia has been the most common cause of death in recent years. The main cause of the bleeding is undetected rupture of the uterus, which is frequently concealed behind the clinical diagnosis of circulatory failure with cardiac arrest. 2/3 of the observed cases in the present series are considered to have been avoidable. It is recommended that in future every single reported case of maternal death in Austria is analyzed and the cause of death and the degree of avoidability discussed as is done in England."}
{"id": "PMID:1087072", "title": "[Fetal haemoglobin levels in acquired and congenital haematological diseases and malignant tumours in adults (author's transl)].", "content": "Hb F values were estimated in 336 adults on 525 occasions. A variable elevation appeared not only in patients with a variety of haematological diseases, but also in conjunction with malignant neoplasms, liver disease etc. The highest values were encountered in cases of pernicious anaemia, haemolytic anaemia, panmyelopathy and plasmocytoma. The determination of Hb F in peripheral blood smears by means of this extremely simple elution method (Betke and Kleihauer) represents a useful tool in the diagnosis of haematological disorders in adults, especially the haemoglobinopathies. The possible causes of this biochemical redifferentiation of erythropoiesis are discussed.", "contents": "[Fetal haemoglobin levels in acquired and congenital haematological diseases and malignant tumours in adults (author's transl)]. Hb F values were estimated in 336 adults on 525 occasions. A variable elevation appeared not only in patients with a variety of haematological diseases, but also in conjunction with malignant neoplasms, liver disease etc. The highest values were encountered in cases of pernicious anaemia, haemolytic anaemia, panmyelopathy and plasmocytoma. The determination of Hb F in peripheral blood smears by means of this extremely simple elution method (Betke and Kleihauer) represents a useful tool in the diagnosis of haematological disorders in adults, especially the haemoglobinopathies. The possible causes of this biochemical redifferentiation of erythropoiesis are discussed."}
{"id": "PMID:1087079", "title": "[Gastrointestinal hemorrhage in the internal medicine cases of a district hospital. Retrospective study from the years 1965-1974].", "content": "In the period between 1965-1974 564 patients with gastrointestinal hemorrhages were admitted in the medical clinic of the District Hospital St. Georg Leipzig. Of them 112 patients (20%) were at once or after failure of a conservative therapy admitted in the surgical clinic for operation. 82 patients died (18%) of the conservatively treated patients). The most frequent sources of haemorrhage duodenal ulcers with 32%, ventricular ulcers with 25% and carcinomas of the stomach with 19%. In 13% of the patients haemorrhages induced by drugs could be assumed. In 13% the case were recidivation haemorrhages. Severe gastrointestinal haemorrhages were observed only in 4% of the patients and consequently were less important in our patients. A further improvement of the diagnostic exactness is possible by perfection of the early diagnosis (emergency gastroscopy and angiography), which may be important with regard to a surgical intervention.", "contents": "[Gastrointestinal hemorrhage in the internal medicine cases of a district hospital. Retrospective study from the years 1965-1974]. In the period between 1965-1974 564 patients with gastrointestinal hemorrhages were admitted in the medical clinic of the District Hospital St. Georg Leipzig. Of them 112 patients (20%) were at once or after failure of a conservative therapy admitted in the surgical clinic for operation. 82 patients died (18%) of the conservatively treated patients). The most frequent sources of haemorrhage duodenal ulcers with 32%, ventricular ulcers with 25% and carcinomas of the stomach with 19%. In 13% of the patients haemorrhages induced by drugs could be assumed. In 13% the case were recidivation haemorrhages. Severe gastrointestinal haemorrhages were observed only in 4% of the patients and consequently were less important in our patients. A further improvement of the diagnostic exactness is possible by perfection of the early diagnosis (emergency gastroscopy and angiography), which may be important with regard to a surgical intervention."}
{"id": "PMID:1087080", "title": "[Retrograde perfusion of the coronary sinus].", "content": "The investigations by Ratajczak-Pakalska showed peculiarities of cardiac venous circulation which make retrograde perfusion possible. Microcirculation is flowing in two directions, especially in diffuse cardiosclerosis. The results of the authors' animal experiments prove that the method of retrograde perfusion of coronary veins might be used ultimately in these cases.", "contents": "[Retrograde perfusion of the coronary sinus]. The investigations by Ratajczak-Pakalska showed peculiarities of cardiac venous circulation which make retrograde perfusion possible. Microcirculation is flowing in two directions, especially in diffuse cardiosclerosis. The results of the authors' animal experiments prove that the method of retrograde perfusion of coronary veins might be used ultimately in these cases."}
{"id": "PMID:1087089", "title": "[Cerebrospinal fluid T- and B-lymphocytes in serous meningitis].", "content": "By means of PHA-tests and rosette-formation the authors detected in the CSF of patients with serous meningitis T- and B-lymphocytes. They assume that in serous meningitis the penetration of alien antigens into the subarachnoidal space facilitates the migration of T- and B-lymphocytes into the CSF from the lymphoid organs and peripheral blood. On the grounds of these data the authors propose a new concept which assumes that besides the hematoenecephalic barrier, there is also an immunological brain barrier, the morphofunctional basis of which is the CSF with circulating T- and B-lymphocytes and immunoglobulins of different classes.", "contents": "[Cerebrospinal fluid T- and B-lymphocytes in serous meningitis]. By means of PHA-tests and rosette-formation the authors detected in the CSF of patients with serous meningitis T- and B-lymphocytes. They assume that in serous meningitis the penetration of alien antigens into the subarachnoidal space facilitates the migration of T- and B-lymphocytes into the CSF from the lymphoid organs and peripheral blood. On the grounds of these data the authors propose a new concept which assumes that besides the hematoenecephalic barrier, there is also an immunological brain barrier, the morphofunctional basis of which is the CSF with circulating T- and B-lymphocytes and immunoglobulins of different classes."}
{"id": "PMID:1087090", "title": "Epidemiological background to diabetes.", "content": "Earlier seasonal inoidence studies indicated that juvenile diabetes is acquired mainly in the autumn and winter months, and more recent and extensive further studies conducted throughout Great Britain have confirmed these earlier findings. Studies relating the incidence of the disease to age among juveniles suggest that an initiating factor, probably viral in origin, is operative in early childhood and that after a latent period, a precipitating factor may then uncover the disease. This might explain the peak of juvenile diabetes which appears at age 11, in Britain. Studies on families in which diabetes has appeared almost simultaneously in two or more members, lends some support to the view that the disease may be precipitated by some infective process.", "contents": "Epidemiological background to diabetes. Earlier seasonal inoidence studies indicated that juvenile diabetes is acquired mainly in the autumn and winter months, and more recent and extensive further studies conducted throughout Great Britain have confirmed these earlier findings. Studies relating the incidence of the disease to age among juveniles suggest that an initiating factor, probably viral in origin, is operative in early childhood and that after a latent period, a precipitating factor may then uncover the disease. This might explain the peak of juvenile diabetes which appears at age 11, in Britain. Studies on families in which diabetes has appeared almost simultaneously in two or more members, lends some support to the view that the disease may be precipitated by some infective process."}
{"id": "PMID:1087092", "title": "Clinical significance of anti-insulin-antibodies.", "content": "Treatment of diabetic patients with common available insulin preparations in most cases results in formation of immuno-globulins called anti-insulin-antibodies. During the last 10-15 years it has been established, that these immuno-globulins, especially IgE, may be responsible for allergical manifestations. Moreover it has been established, that immuno-globulins, especially IgM, may be responsible for insulin resistance in many cases. It is concluded, that anti-insulin-antibodies also are important for the insulin requirement in non-resistant diabetics. The investigations could not reveal any significance of anti-insulin-antibodies for the degree of regulation. The remission period has been defined, 45 per cent of patients examined fulfilled the criteria for remission. The investigations support the assumption, that anti-insulin-antibodies may shorten the remission period, probably due to neutralizing effect upon the endogenous insulin supply. Finally our findings are in accordance with the view, that insulin-anti-insulin-antibody complexes might deteriorate late diabetic vascular complications.", "contents": "Clinical significance of anti-insulin-antibodies. Treatment of diabetic patients with common available insulin preparations in most cases results in formation of immuno-globulins called anti-insulin-antibodies. During the last 10-15 years it has been established, that these immuno-globulins, especially IgE, may be responsible for allergical manifestations. Moreover it has been established, that immuno-globulins, especially IgM, may be responsible for insulin resistance in many cases. It is concluded, that anti-insulin-antibodies also are important for the insulin requirement in non-resistant diabetics. The investigations could not reveal any significance of anti-insulin-antibodies for the degree of regulation. The remission period has been defined, 45 per cent of patients examined fulfilled the criteria for remission. The investigations support the assumption, that anti-insulin-antibodies may shorten the remission period, probably due to neutralizing effect upon the endogenous insulin supply. Finally our findings are in accordance with the view, that insulin-anti-insulin-antibody complexes might deteriorate late diabetic vascular complications."}
{"id": "PMID:1087093", "title": "The influence of insulin and insulin antibodies on the glomerular structure.", "content": "Guinea pigs and rabbits were treated with heterologous insulin preparations of different purity for 3 months without Freund's adjuvant. The insulin binding capacity of the serum, as well as the light and electron microscopic structure of the glomeruli were studied and the glomerular structure was analyzed with quantitative methods. By a longer treatment with heterologous commercial insulin preparations a proliferation of mesangial cells and a thickening of the mesangium matrix were showed. Furthermore, hump-like basement membrane protuberances were found in the glomerular capillary wall. Their frequency increased when more insulin antibodies were formed. Rabbits treated with MC insulin showed no significant antibody formation and no significant changes in the glomerular structure. Otherwise, in animals treated with the impurities of the insulin preparations (a+b component) a marked increase in the basement membrane protuberances was demonstrable accompanied by a higher antibody titer. An increase in the mesangial cell density as well as an increase in the mesangial volume were also present. The results confirm the findings that a contaminating protein in crystalline insulin to be responsible for the antigenic properties.", "contents": "The influence of insulin and insulin antibodies on the glomerular structure. Guinea pigs and rabbits were treated with heterologous insulin preparations of different purity for 3 months without Freund's adjuvant. The insulin binding capacity of the serum, as well as the light and electron microscopic structure of the glomeruli were studied and the glomerular structure was analyzed with quantitative methods. By a longer treatment with heterologous commercial insulin preparations a proliferation of mesangial cells and a thickening of the mesangium matrix were showed. Furthermore, hump-like basement membrane protuberances were found in the glomerular capillary wall. Their frequency increased when more insulin antibodies were formed. Rabbits treated with MC insulin showed no significant antibody formation and no significant changes in the glomerular structure. Otherwise, in animals treated with the impurities of the insulin preparations (a+b component) a marked increase in the basement membrane protuberances was demonstrable accompanied by a higher antibody titer. An increase in the mesangial cell density as well as an increase in the mesangial volume were also present. The results confirm the findings that a contaminating protein in crystalline insulin to be responsible for the antigenic properties."}
{"id": "PMID:1087088", "title": "[Assessment of the reinforcing properties of self stimulation in rats using the \"discordance\" index].", "content": "Histogramms of the duration of pedal pressing (PDH) as a function of stimulation parameters, were studied in fifteen rats with electrodes inserted in the lateral hypothalamus and with fixed series of brain stimulation. The \"discordance\" parameter was used to estimate the changes in PDH modes relative to the moment of the end of brain stimulation. It was found that an increase in the stimulation charge brought about a gradual replacement of positive discordance (the duration of pressing exceeds that of stimulation) by a negative one (duration of pressing is shorter than that of stimulation) and a rise of negative discordance. It is assumed that in the case of positive discordance the duration of the total brain stimulation possesses reinforcing properties, while in the case of negative discordance the beginning of the stimulation possesses reinforcing properties, and its continuation becomes negative.", "contents": "[Assessment of the reinforcing properties of self stimulation in rats using the \"discordance\" index]. Histogramms of the duration of pedal pressing (PDH) as a function of stimulation parameters, were studied in fifteen rats with electrodes inserted in the lateral hypothalamus and with fixed series of brain stimulation. The \"discordance\" parameter was used to estimate the changes in PDH modes relative to the moment of the end of brain stimulation. It was found that an increase in the stimulation charge brought about a gradual replacement of positive discordance (the duration of pressing exceeds that of stimulation) by a negative one (duration of pressing is shorter than that of stimulation) and a rise of negative discordance. It is assumed that in the case of positive discordance the duration of the total brain stimulation possesses reinforcing properties, while in the case of negative discordance the beginning of the stimulation possesses reinforcing properties, and its continuation becomes negative."}
{"id": "PMID:1087095", "title": "Cellular interactions in the proliferative response of human circulating lymphocytes to PHA-analysis of the impaired response in old age.", "content": "Peripheral blood lymphocytes (PBL) from old subjects were less responsive to PHA than those from young controls, in both groups the PBL content of macrophages, T and B cells were comparable. Macrophages were identified as peroxydase containing cells, rosette formation with sheep red blood cell was used as a T cell marker and immunoglobulin bearing lymphocytes were considered to be B cells. PBL were separated in two subpopulations of purified T cells (F1 and F2) differing in their responsiveness to PHA and in their ability to cooperate with B cells. Purified B cells had a synergistic effect on the F1 response to PHA whereas they were often suppressive for the corresponding F2 response. Peroxydase containing cells enhanced the response of unfractionated total PBL and of purified T cells in the young subjects. The depressed response of the old age group could be related mainly to a suppressive effect of monocytes and a decreased circulating pool of F1 T cells.", "contents": "Cellular interactions in the proliferative response of human circulating lymphocytes to PHA-analysis of the impaired response in old age. Peripheral blood lymphocytes (PBL) from old subjects were less responsive to PHA than those from young controls, in both groups the PBL content of macrophages, T and B cells were comparable. Macrophages were identified as peroxydase containing cells, rosette formation with sheep red blood cell was used as a T cell marker and immunoglobulin bearing lymphocytes were considered to be B cells. PBL were separated in two subpopulations of purified T cells (F1 and F2) differing in their responsiveness to PHA and in their ability to cooperate with B cells. Purified B cells had a synergistic effect on the F1 response to PHA whereas they were often suppressive for the corresponding F2 response. Peroxydase containing cells enhanced the response of unfractionated total PBL and of purified T cells in the young subjects. The depressed response of the old age group could be related mainly to a suppressive effect of monocytes and a decreased circulating pool of F1 T cells."}
{"id": "PMID:1087096", "title": "[The natural course of coronary heart disease].", "content": "For a critical evaluation regarding surgical therapy of coronary heart disease, we quote from important publications to the following points: Early mortality due to surgical treatment, by estimating the severity of the disease as determinated by the criterions of the New York Heart Association, or by the number of the diseased coronary vessels, or by important parameters of the function of the left ventricle (0% to 12%). Frequency of acute myocardial infarction, intra-or postoperatively (10% to 15% of the survivers, 50% of the lethal cases). Frequency of occlusion, of an aorto-coronary saphenous vein bypass (up to 30%). Amelioration of ischemic heart pain by surgical treatment (up to 90%). Prevention of the progression of coronary heart disease (not proved). Natural history of patients with coronary heart disease without surgical treatment, by estimating the number of the diseased vessels (2% for one-, 6% for two-, and 10% for three vessel diseases; 30% mortality rate due to stenosis of the left main coronary artery). Comparison of the mortality rates of surgically treated patients against those without surgery for coronary heart disease with equal severity (no difference). We investigated the connection between the severity of coronary heart disease (determinated by coronary angiography), and the mortality rate in our own patient material. A fundamental conformity appeared, with the results as quoted in the literature. Finally we difined the indications for surgical treatment of coronary heart disease due to angina pectoris, which cannot be managed conservatively and which makes a normal live difficult or impossible. Moreover, the function of the left ventricle should be fairly good. An absolute indication is a stenosis of the left main coronary artery.", "contents": "[The natural course of coronary heart disease]. For a critical evaluation regarding surgical therapy of coronary heart disease, we quote from important publications to the following points: Early mortality due to surgical treatment, by estimating the severity of the disease as determinated by the criterions of the New York Heart Association, or by the number of the diseased coronary vessels, or by important parameters of the function of the left ventricle (0% to 12%). Frequency of acute myocardial infarction, intra-or postoperatively (10% to 15% of the survivers, 50% of the lethal cases). Frequency of occlusion, of an aorto-coronary saphenous vein bypass (up to 30%). Amelioration of ischemic heart pain by surgical treatment (up to 90%). Prevention of the progression of coronary heart disease (not proved). Natural history of patients with coronary heart disease without surgical treatment, by estimating the number of the diseased vessels (2% for one-, 6% for two-, and 10% for three vessel diseases; 30% mortality rate due to stenosis of the left main coronary artery). Comparison of the mortality rates of surgically treated patients against those without surgery for coronary heart disease with equal severity (no difference). We investigated the connection between the severity of coronary heart disease (determinated by coronary angiography), and the mortality rate in our own patient material. A fundamental conformity appeared, with the results as quoted in the literature. Finally we difined the indications for surgical treatment of coronary heart disease due to angina pectoris, which cannot be managed conservatively and which makes a normal live difficult or impossible. Moreover, the function of the left ventricle should be fairly good. An absolute indication is a stenosis of the left main coronary artery."}
{"id": "PMID:1087098", "title": "Tone audiometry control of children treated for meningitis with large intravenous doses of ampicillin.", "content": "Thirty-seven children treated for bacterial meningitis with ampicillin in high doses were followed up with audiometric control. Defects were recorded in 6 cases. Two of these were ascribed to chronic otosalpingitis. In 4 patients with sensorineural hearing loss, 3 were unilateral, and in only one case was the damage bilateral. Even here there was a previous history of hearing loss. A suggested ototoxicity of ampicillin could not be confirmed.", "contents": "Tone audiometry control of children treated for meningitis with large intravenous doses of ampicillin. Thirty-seven children treated for bacterial meningitis with ampicillin in high doses were followed up with audiometric control. Defects were recorded in 6 cases. Two of these were ascribed to chronic otosalpingitis. In 4 patients with sensorineural hearing loss, 3 were unilateral, and in only one case was the damage bilateral. Even here there was a previous history of hearing loss. A suggested ototoxicity of ampicillin could not be confirmed."}
{"id": "PMID:1087099", "title": "The taxonomy of haemophili isolated from conjunctivae.", "content": "The paper provides a description of 112 Haemophilus strains isolated from cases of conjunctivitis in Egypt, Tunisia, Denmark, and the U.S.A., and aims at a clarification of the taxonomy of haemophili implicated in conjunctivitis. Although the study confirms the diversity of haemophili which can be isolated from inflamed conjunctivae, the vast majority of strains could be assigned to either biotype II or III of H. influenzae. Thirteen strains possessed all the characteristics of the Koch-Weeks bacillus. However, judged by the findings the recognition of the Koch-Weeks bacillus as a separate species does not seem tenable. It is suggested that this organism is considered a haemagglutinating variety of H. influenzae biotype III. The study indicates that the haemagglutinating ability per se, which can be found in strains of several taxa of Haemophilus, is associated with a special pathogenic potential.", "contents": "The taxonomy of haemophili isolated from conjunctivae. The paper provides a description of 112 Haemophilus strains isolated from cases of conjunctivitis in Egypt, Tunisia, Denmark, and the U.S.A., and aims at a clarification of the taxonomy of haemophili implicated in conjunctivitis. Although the study confirms the diversity of haemophili which can be isolated from inflamed conjunctivae, the vast majority of strains could be assigned to either biotype II or III of H. influenzae. Thirteen strains possessed all the characteristics of the Koch-Weeks bacillus. However, judged by the findings the recognition of the Koch-Weeks bacillus as a separate species does not seem tenable. It is suggested that this organism is considered a haemagglutinating variety of H. influenzae biotype III. The study indicates that the haemagglutinating ability per se, which can be found in strains of several taxa of Haemophilus, is associated with a special pathogenic potential."}
{"id": "PMID:1087100", "title": "Differential effect of cortisol on thymocytes from mice of different ages.", "content": "The influence of cortisol in vivo and in vitro was studied in thymus sections, suspensions, and smears obtained from new-born, 6 days old, and 30 days old mice. Histology as well as differential counts revealed that new-born thymocytes are far more sensitive to the cytotoxic effect of cortisol than thymocytes from older animals, and thymocytes from 30 days old mice are more sensitive than cells from 6 days old animals. Thus, the number of pyknotic cells in the thymus of new-born and 30 days old mice increased up to 20 per cent 6 h after injection of cortisol compared to 10 per cent in 6 days old animals. At 24 h after injection, the per cent of pyknotic cells in the thymus of new-born mice was 50 compared with about 1 in the two older age-groups. Supravital dye exclusion showed that thymocytes in 6 days old mice are less sensitive to the cytolytic action of cortisol in vivo than thymocytes from new-born and 30 days old mice. Incorporation of 3H-TdR and 3H-UdR into thymocyte DNA and RNA, respectively, was inhibited in all groups after treatment in vivo. DNA-synthesis of thymocytes in new-born animals was inhibited in all groups after treatment in vivo. DNA-synthesis of thymocytes in new born animals was inhibited more than DNA-synthesis in thymocytes of older animals, whereas RNA-synthesis was inhibited to nearly the same extend in the three groups studied. Incubation in vitro with 10(-6)M cortisol inhibited the DNA-synthesis to the same extent in thymocytes from the three age groups studied whereas the inhibiting effect of 10(-5)M cortisol on thymocytes from 6 and 30 days old animals was more intense than that on cells in new-born mice. The RNA-synthesis of thymocytes from 6 days old mice incubated in 10(-5)M cortisol was inhibited far less than RNA-synthesis in cells from new-born and 30 days old mice. The experiments reported, showing differential effects of cortisol on thymocytes obtained from mice of different ages, may reflect different stages of thymocytes maturation during the process of ontogenesis.", "contents": "Differential effect of cortisol on thymocytes from mice of different ages. The influence of cortisol in vivo and in vitro was studied in thymus sections, suspensions, and smears obtained from new-born, 6 days old, and 30 days old mice. Histology as well as differential counts revealed that new-born thymocytes are far more sensitive to the cytotoxic effect of cortisol than thymocytes from older animals, and thymocytes from 30 days old mice are more sensitive than cells from 6 days old animals. Thus, the number of pyknotic cells in the thymus of new-born and 30 days old mice increased up to 20 per cent 6 h after injection of cortisol compared to 10 per cent in 6 days old animals. At 24 h after injection, the per cent of pyknotic cells in the thymus of new-born mice was 50 compared with about 1 in the two older age-groups. Supravital dye exclusion showed that thymocytes in 6 days old mice are less sensitive to the cytolytic action of cortisol in vivo than thymocytes from new-born and 30 days old mice. Incorporation of 3H-TdR and 3H-UdR into thymocyte DNA and RNA, respectively, was inhibited in all groups after treatment in vivo. DNA-synthesis of thymocytes in new-born animals was inhibited in all groups after treatment in vivo. DNA-synthesis of thymocytes in new born animals was inhibited more than DNA-synthesis in thymocytes of older animals, whereas RNA-synthesis was inhibited to nearly the same extend in the three groups studied. Incubation in vitro with 10(-6)M cortisol inhibited the DNA-synthesis to the same extent in thymocytes from the three age groups studied whereas the inhibiting effect of 10(-5)M cortisol on thymocytes from 6 and 30 days old animals was more intense than that on cells in new-born mice. The RNA-synthesis of thymocytes from 6 days old mice incubated in 10(-5)M cortisol was inhibited far less than RNA-synthesis in cells from new-born and 30 days old mice. The experiments reported, showing differential effects of cortisol on thymocytes obtained from mice of different ages, may reflect different stages of thymocytes maturation during the process of ontogenesis."}
{"id": "PMID:1087101", "title": "Antinuclear factors in sera from healthy blood donors.", "content": "Sera obtained from 466 healthy blood donors were investigated for presence of granulocyte-specific and organ-nonspecific antinuclear factors of the IgM, IgA and IgG classes, 110 sera also for complement-fixing and IgD class antinuclear factors. When undiluted sera were studied, most sera were found to contain antinuclear factors of one or more immunoglobulin classes (77 per cent). IgM, IgA and IgG antinuclear factors were found in 53, 44 and 14 per cent, respectively. At dilution 1:16, antinuclear factors of the IgM class were still detected in 16 per cent of the sera, while IgA and IgC antinuclear factors wre demonstrated in 6 and 2 per cent, respectively. Organ-nonspecific antinuclear factors were clearly more common than granulocyte-specific antinuclear factors. No serum contained complement-fixing or IgD class antinuclear factors. This study indicates the importance of using qualitative as well as quantitative techniques to distinguish between antinuclear factors in health and disease.", "contents": "Antinuclear factors in sera from healthy blood donors. Sera obtained from 466 healthy blood donors were investigated for presence of granulocyte-specific and organ-nonspecific antinuclear factors of the IgM, IgA and IgG classes, 110 sera also for complement-fixing and IgD class antinuclear factors. When undiluted sera were studied, most sera were found to contain antinuclear factors of one or more immunoglobulin classes (77 per cent). IgM, IgA and IgG antinuclear factors were found in 53, 44 and 14 per cent, respectively. At dilution 1:16, antinuclear factors of the IgM class were still detected in 16 per cent of the sera, while IgA and IgC antinuclear factors wre demonstrated in 6 and 2 per cent, respectively. Organ-nonspecific antinuclear factors were clearly more common than granulocyte-specific antinuclear factors. No serum contained complement-fixing or IgD class antinuclear factors. This study indicates the importance of using qualitative as well as quantitative techniques to distinguish between antinuclear factors in health and disease."}
{"id": "PMID:1087102", "title": "The cytotoxicity of specifically sensitized lymphocytes from mouse strains of varying H-2 specificities on LCM virus-infected L cells.", "content": "During an LCM virus infection in mice, cytotoxic T lymphocytes appear to be directed against target cells infected with the virus. This cytotoxic reaction is restricted by the H-2 histo-compatibility between effector and target cells. To analyse this restriction, the cytotoxic effect of in vivo sensitized spleen cells from mice of various H-2 specificities on LCM virus-infecte of in vivo sensitized spleen cells from mice of various H-2 specificities on LCM virus-infected L cells (H-2k) was studied in vitro. When spleen cells from strains identical with C3H (H-2k) and L cells at the K as well as at the D locus were used as effector cells, the degree of lysis was of the same order as that obtained by C3H effector cells, whereas strains different at both loci did not respond significantly. The A/J and B10A strains, both identical with the L cells at the K locus but different at the D locus, gave low or moderate responses. C3H-H-20 lymphocytes, identical with the targets at the D but not at the K locus, showed high degrees of cytotoxicity although the level obtained by strains histocompatible with L cells at both loci was never reached. Effector cells from the F1 hybrids C3H/HeJ X BALB/c and C3H/HeJ X DBA both responded poorly in spite of the fact that one of their haplotypes carries the same marker loci as the L cells. This finding is in contrast with that obtained in other laboratories where the LCM virus system and other systems were studied. A point is made of the design and the evaluation of the experiments.", "contents": "The cytotoxicity of specifically sensitized lymphocytes from mouse strains of varying H-2 specificities on LCM virus-infected L cells. During an LCM virus infection in mice, cytotoxic T lymphocytes appear to be directed against target cells infected with the virus. This cytotoxic reaction is restricted by the H-2 histo-compatibility between effector and target cells. To analyse this restriction, the cytotoxic effect of in vivo sensitized spleen cells from mice of various H-2 specificities on LCM virus-infecte of in vivo sensitized spleen cells from mice of various H-2 specificities on LCM virus-infected L cells (H-2k) was studied in vitro. When spleen cells from strains identical with C3H (H-2k) and L cells at the K as well as at the D locus were used as effector cells, the degree of lysis was of the same order as that obtained by C3H effector cells, whereas strains different at both loci did not respond significantly. The A/J and B10A strains, both identical with the L cells at the K locus but different at the D locus, gave low or moderate responses. C3H-H-20 lymphocytes, identical with the targets at the D but not at the K locus, showed high degrees of cytotoxicity although the level obtained by strains histocompatible with L cells at both loci was never reached. Effector cells from the F1 hybrids C3H/HeJ X BALB/c and C3H/HeJ X DBA both responded poorly in spite of the fact that one of their haplotypes carries the same marker loci as the L cells. This finding is in contrast with that obtained in other laboratories where the LCM virus system and other systems were studied. A point is made of the design and the evaluation of the experiments."}
{"id": "PMID:1087097", "title": "Serological studies in acute maxillary sinusitis.", "content": "A comparison was performed between bacteriological and serological findings in 97 patients suffering from acute maxillary sinusitis. A significant titre change (larger than or equal to 2 titre steps) of complement-fixing antibodies to H. influenzae was demonstrated in 15 patients. H. influenzae was demonstrated in aspirated sinus secretions from 7 of these 15 patients. Further, titre changes to Neisseria catarrhalis were demonstrated in 25 patients. No significant titre changes in anti-streptolysin and anti-staphylolysin titres were demonstrated. The results are discussed briefly.", "contents": "Serological studies in acute maxillary sinusitis. A comparison was performed between bacteriological and serological findings in 97 patients suffering from acute maxillary sinusitis. A significant titre change (larger than or equal to 2 titre steps) of complement-fixing antibodies to H. influenzae was demonstrated in 15 patients. H. influenzae was demonstrated in aspirated sinus secretions from 7 of these 15 patients. Further, titre changes to Neisseria catarrhalis were demonstrated in 25 patients. No significant titre changes in anti-streptolysin and anti-staphylolysin titres were demonstrated. The results are discussed briefly."}
{"id": "PMID:1087103", "title": "Some observations in treatment stimulation of epilepsy.", "content": "In a group of 10 patients suffering from epilepsy therapeutic stimulation was applied by means of chronic electrodes and a technical stimulator three times in nucleus dentatus, five times in nucleus caudatus, once in both structures simultaneously. Once chronic electrodes were introduced into the amygdalo-hippocampal complex and non-specific thalanic system bilaterally. One patient was stimulated by means of a pacemaker and once by normal rhythm from the hypothalamus of the same patient using magnetic tape. The patients were subjected to stimulations once a day for a period of 1-8 days. The results obtained are being discussed with respect to Goddard's kindling phenomenon.", "contents": "Some observations in treatment stimulation of epilepsy. In a group of 10 patients suffering from epilepsy therapeutic stimulation was applied by means of chronic electrodes and a technical stimulator three times in nucleus dentatus, five times in nucleus caudatus, once in both structures simultaneously. Once chronic electrodes were introduced into the amygdalo-hippocampal complex and non-specific thalanic system bilaterally. One patient was stimulated by means of a pacemaker and once by normal rhythm from the hypothalamus of the same patient using magnetic tape. The patients were subjected to stimulations once a day for a period of 1-8 days. The results obtained are being discussed with respect to Goddard's kindling phenomenon."}
{"id": "PMID:1087108", "title": "The effect of coronary bypass surgery on exercise-induced ventricular arrhythmias.", "content": "Ninety-one patients with angiographically proved coronary artery disease and stable angina were randomly assigned into surgical and medical therapy. Graded exercise tests were performed on entry into the study and repeated in 1 year. Ventricular arrhythmias during exercise and 8 minutes of recovery were studied. Arrhythmias were graded on a scale of 0 to 7 by their presumed severity. On entry, both groups were similar in the severity of coronary disease, exercise capacity, and frequency and severity of exercise-induced ventricular arrhythmias. At 1 year, the frequency and severity of arrhythmias remained unchanged in both groups, whereas the surgically treated patients showed a marked improvement in their exercise capacity (p less than 0.005). The medically treated patients had a slight deterioration in their work capacity which, however, did not achieve statistical significance (p = 0.08). Twelve patients died suddenly. In seven medically treated patients who died suddenly, the frequency and severity of ventricular arrythmias on exercise were not different from those of the rest of the medical patients. In the five surgically treated patients who died suddenly, one had multiform premature ventricular beats, a second developed ventricular fibrillation (2 years before dying suddenly), and a third had no arrhythmias during exercise. Two died before the 1 year evaluation. Successful coronary surgery improves exercise capacity without decreasing associated ventricular arrhythmias. Exercise-induced ventricular arrhythmias, with the exception of ventricular fibrillation, may not be closely associated with the risk of sudden death.", "contents": "The effect of coronary bypass surgery on exercise-induced ventricular arrhythmias. Ninety-one patients with angiographically proved coronary artery disease and stable angina were randomly assigned into surgical and medical therapy. Graded exercise tests were performed on entry into the study and repeated in 1 year. Ventricular arrhythmias during exercise and 8 minutes of recovery were studied. Arrhythmias were graded on a scale of 0 to 7 by their presumed severity. On entry, both groups were similar in the severity of coronary disease, exercise capacity, and frequency and severity of exercise-induced ventricular arrhythmias. At 1 year, the frequency and severity of arrhythmias remained unchanged in both groups, whereas the surgically treated patients showed a marked improvement in their exercise capacity (p less than 0.005). The medically treated patients had a slight deterioration in their work capacity which, however, did not achieve statistical significance (p = 0.08). Twelve patients died suddenly. In seven medically treated patients who died suddenly, the frequency and severity of ventricular arrythmias on exercise were not different from those of the rest of the medical patients. In the five surgically treated patients who died suddenly, one had multiform premature ventricular beats, a second developed ventricular fibrillation (2 years before dying suddenly), and a third had no arrhythmias during exercise. Two died before the 1 year evaluation. Successful coronary surgery improves exercise capacity without decreasing associated ventricular arrhythmias. Exercise-induced ventricular arrhythmias, with the exception of ventricular fibrillation, may not be closely associated with the risk of sudden death."}
{"id": "PMID:1087109", "title": "Vein graft patency and intimal proliferation after aortocoronary bypass: early and long-term angiopathologic correlations.", "content": "To determine the clinical significance of intimal proliferation as a cause of aortocoronary bypass vein graft obstruction, 492 vein grafts from 281 patients were studied 0 to 75 months postoperatively. All grafts had been inserted between 1968 and 1975 by one surgeon using one technique. The graft patency rate was 92 percent (55 to 60) in the first month; 91 percent (49 of 54) at 1 to 3 months; 84 percent (37 to 44) at 4 to 6 months; 77 percent (33 of 43) at 7 to 12 months; 81 percent (113 of 140) at 13 to 24 months; 82 percent (59 of 72) at 25 to 36 months; and 84 percent (66 of 79) at 37 to 75 months. Vein graft samples were obtained from 41 patients: In 27 patients with 51 grafts (early group), they were obtained 0 to 30 days (mean 14 days) postoperatively; in 14 patients with 27 grafts (late group) they were obtained 7 to 75 months (mean 34 months) postoperatively. Intimal proliferation was graded 1 to 4 corresponding to an intima/media thickness ratio of 1,2,3 or 4, respectively. In the early group, all 51 vein grafts howed grade 1 to 2 intimal proliferation; 5 of these grafts were occluded, all as a result of recent thrombosis. In the late group, 17 of the 27 grafts were studied histologically. All patent vein grafts showed grade 2 to 3 intimal prolferation. Four vein grafts were occluded but only one as a result of grade 4 intimal proliferation. In 14 patients in the late group, angiograms performed shortly before vein graft samples were obtained revealed 14 patent and 4 occluded vein grafts. Ten of the 14 patent vein grafts showed grade 2 to 3 intimal proliferation but were of uniformly good caliber angiographically (graft/artery ratio more than 1.5).", "contents": "Vein graft patency and intimal proliferation after aortocoronary bypass: early and long-term angiopathologic correlations. To determine the clinical significance of intimal proliferation as a cause of aortocoronary bypass vein graft obstruction, 492 vein grafts from 281 patients were studied 0 to 75 months postoperatively. All grafts had been inserted between 1968 and 1975 by one surgeon using one technique. The graft patency rate was 92 percent (55 to 60) in the first month; 91 percent (49 of 54) at 1 to 3 months; 84 percent (37 to 44) at 4 to 6 months; 77 percent (33 of 43) at 7 to 12 months; 81 percent (113 of 140) at 13 to 24 months; 82 percent (59 of 72) at 25 to 36 months; and 84 percent (66 of 79) at 37 to 75 months. Vein graft samples were obtained from 41 patients: In 27 patients with 51 grafts (early group), they were obtained 0 to 30 days (mean 14 days) postoperatively; in 14 patients with 27 grafts (late group) they were obtained 7 to 75 months (mean 34 months) postoperatively. Intimal proliferation was graded 1 to 4 corresponding to an intima/media thickness ratio of 1,2,3 or 4, respectively. In the early group, all 51 vein grafts howed grade 1 to 2 intimal proliferation; 5 of these grafts were occluded, all as a result of recent thrombosis. In the late group, 17 of the 27 grafts were studied histologically. All patent vein grafts showed grade 2 to 3 intimal prolferation. Four vein grafts were occluded but only one as a result of grade 4 intimal proliferation. In 14 patients in the late group, angiograms performed shortly before vein graft samples were obtained revealed 14 patent and 4 occluded vein grafts. Ten of the 14 patent vein grafts showed grade 2 to 3 intimal proliferation but were of uniformly good caliber angiographically (graft/artery ratio more than 1.5)."}
{"id": "PMID:1087110", "title": "Haemophilus influenzae meningitis: an evolving therapeutic regimen.", "content": "Ampicillin sodium has been the drug of choice in the treatment of Haemophilus influenzae meningitis. The development of ampicillin-resistant strains forces the clinician to focus on alternative therapies. We describe two patients in whom neutropenia was noted secondary to chloramphenicol administration, and streptomycin sulfate and sulfonamides were employed. An historical perspective summarizing the evolution of available therapeutic regimens is presented.", "contents": "Haemophilus influenzae meningitis: an evolving therapeutic regimen. Ampicillin sodium has been the drug of choice in the treatment of Haemophilus influenzae meningitis. The development of ampicillin-resistant strains forces the clinician to focus on alternative therapies. We describe two patients in whom neutropenia was noted secondary to chloramphenicol administration, and streptomycin sulfate and sulfonamides were employed. An historical perspective summarizing the evolution of available therapeutic regimens is presented."}
{"id": "PMID:1087111", "title": "Familial congenital monochromatism, cataracts, and sensorineural deafness.", "content": "Two sisters had diagnoses of congenital monochromatism, cataracts, bilateral nonprogressive sensorineural deafness, and hyperinsulinism in both, and labyrinthine dysfunction in one. This recessively inherited condition is added to the growing number of syndromes in which one of the features may be a disturbance of hypothalamic function.", "contents": "Familial congenital monochromatism, cataracts, and sensorineural deafness. Two sisters had diagnoses of congenital monochromatism, cataracts, bilateral nonprogressive sensorineural deafness, and hyperinsulinism in both, and labyrinthine dysfunction in one. This recessively inherited condition is added to the growing number of syndromes in which one of the features may be a disturbance of hypothalamic function."}
{"id": "PMID:1087113", "title": "Pancreatic pseudocyst as a cause of gastrointestinal bleeding and hemobilia. A case report.", "content": "A case of hemobilia from a pancreatic pseudocyst which developed after cholecystostomy and aspiration of the pseudocyst, intended to relieve biliary obstruction, is discussed. These previously reported cases are briefly reviewed.", "contents": "Pancreatic pseudocyst as a cause of gastrointestinal bleeding and hemobilia. A case report. A case of hemobilia from a pancreatic pseudocyst which developed after cholecystostomy and aspiration of the pseudocyst, intended to relieve biliary obstruction, is discussed. These previously reported cases are briefly reviewed."}
{"id": "PMID:1087114", "title": "Autoantibodies in chronic gastritis.", "content": "Gastric parietal cell, antinuclear, smooth-muscle, mitochondrial and microsomal antibodies were determined in 12 Chinese patients with chronic atropic gastritis, 15 Chinese patients with chronic (superficial) gastritis, four Chinese patients with acute-on-chronic gastritis and 16 Chinese control subjects. With the exception of a weakly positive gastric parietal cell antibody test in one of the 12 patients with chronic atrophic gastritis, autoantibodies were not found in all the gastritis and contol groups. It is concluded that autoantibodies are rarely present in Chinese patients with chronic gastritis.", "contents": "Autoantibodies in chronic gastritis. Gastric parietal cell, antinuclear, smooth-muscle, mitochondrial and microsomal antibodies were determined in 12 Chinese patients with chronic atropic gastritis, 15 Chinese patients with chronic (superficial) gastritis, four Chinese patients with acute-on-chronic gastritis and 16 Chinese control subjects. With the exception of a weakly positive gastric parietal cell antibody test in one of the 12 patients with chronic atrophic gastritis, autoantibodies were not found in all the gastritis and contol groups. It is concluded that autoantibodies are rarely present in Chinese patients with chronic gastritis."}
{"id": "PMID:1087115", "title": "T and B lymphocytes in leukemia therapy.", "content": "In order to evaluate the effect of radio- and chemotherapy on immunity, T and B lymphocyte surface receptors were studies sequentially in the blood from 28 previously untreated leukemic children. Following the initiation of chemotherapy an increase in the percent T and B cells was noted in the peripheral blood. In association with sanctuary therapy and chemotherapy there was a decrease in the total number of circulating T and B cells and a relative increase in lymphocytes lacking markers. Based on total numbers at remission the reduction in B cells was greater than in T cells, and the most marked changes occurred during sanctuary therapy. A reduction in the mean serum immunoglobulin was associated with decreasing B cell numbers.", "contents": "T and B lymphocytes in leukemia therapy. In order to evaluate the effect of radio- and chemotherapy on immunity, T and B lymphocyte surface receptors were studies sequentially in the blood from 28 previously untreated leukemic children. Following the initiation of chemotherapy an increase in the percent T and B cells was noted in the peripheral blood. In association with sanctuary therapy and chemotherapy there was a decrease in the total number of circulating T and B cells and a relative increase in lymphocytes lacking markers. Based on total numbers at remission the reduction in B cells was greater than in T cells, and the most marked changes occurred during sanctuary therapy. A reduction in the mean serum immunoglobulin was associated with decreasing B cell numbers."}
{"id": "PMID:1087116", "title": "Identification of T cell lymphoma tumor antigens on human T cell lines.", "content": "All human T lymphoblast cell lines have been derived from subjects with leukemia secondary to thymic lymphoblastic lymphoma, a T cell malignancy, suggesting that such lines represent established cultures of neoplastic T cells. Based on this observation, we prepared rabbit antisera to T cell line HSB-2, removed reactivity for histocompatibility antigens and normal T cells by absorption with autocthonous B cell line CCRF-SB and normal thymocytes, and tested the absorbed antisera by complement-dependent cytotoxicity against a panel of normal and malignant cells. A representative antiserum reacted with all 4 T cell lines (mean cytotoxic index =56) and with tumor cells from 4 patients with T cell lymphoma (mean cytotoxic index = 50) but did not react with tumor cells from 6 patients with other types of leukemias (mean cytotoxic index = 2), with 3 B cell lines (mean cytotoxic index = 1), normal peripheral blood lymphocytes (mean cytotoxic index = 5), or normal thymocytes (mean cytotoxic index = 6). We conclude that appropriately absorbed antisera to human T cell lines detect T cell lymphoma tumor antigens.", "contents": "Identification of T cell lymphoma tumor antigens on human T cell lines. All human T lymphoblast cell lines have been derived from subjects with leukemia secondary to thymic lymphoblastic lymphoma, a T cell malignancy, suggesting that such lines represent established cultures of neoplastic T cells. Based on this observation, we prepared rabbit antisera to T cell line HSB-2, removed reactivity for histocompatibility antigens and normal T cells by absorption with autocthonous B cell line CCRF-SB and normal thymocytes, and tested the absorbed antisera by complement-dependent cytotoxicity against a panel of normal and malignant cells. A representative antiserum reacted with all 4 T cell lines (mean cytotoxic index =56) and with tumor cells from 4 patients with T cell lymphoma (mean cytotoxic index = 50) but did not react with tumor cells from 6 patients with other types of leukemias (mean cytotoxic index = 2), with 3 B cell lines (mean cytotoxic index = 1), normal peripheral blood lymphocytes (mean cytotoxic index = 5), or normal thymocytes (mean cytotoxic index = 6). We conclude that appropriately absorbed antisera to human T cell lines detect T cell lymphoma tumor antigens."}
{"id": "PMID:1087117", "title": "Actions of ristocetin on platelets.", "content": "The absence of ristocetin-induced platelet aggregation appears to correlate with the platelet defect in von Willebrand's disease, suggesting that this reaction mimics a physiological process. The effect of ristocetin on plasma and on the residual levels of the von Willebrand factor (vWF), Factor VIII procoagulant activity, and Factor VIII-related protein in plasma after aggregation of platelet rich plasma by this agent has been studied in order to further elucidate the mechanism and requirements of this reaction. Ristocetin-induced platelet aggregation causes a consumption of vWF, Factor VIII procoagulant activity, and Factor VIII antigen from the supernatant plasma which is proportional to the number of platelets aggregated. Such a consumption of these factors does not appear to occur after aggregation by other agents. Factor VIII procoagulant activity does not appear necessary for ristocetin-induced platelet aggregation, yet is utilized in this process. These findings support the hypothesis that the molecule associated with Factor VIII procoagulant activity is carried by the molecule necessary for ristocetin-induced platelet aggregation.", "contents": "Actions of ristocetin on platelets. The absence of ristocetin-induced platelet aggregation appears to correlate with the platelet defect in von Willebrand's disease, suggesting that this reaction mimics a physiological process. The effect of ristocetin on plasma and on the residual levels of the von Willebrand factor (vWF), Factor VIII procoagulant activity, and Factor VIII-related protein in plasma after aggregation of platelet rich plasma by this agent has been studied in order to further elucidate the mechanism and requirements of this reaction. Ristocetin-induced platelet aggregation causes a consumption of vWF, Factor VIII procoagulant activity, and Factor VIII antigen from the supernatant plasma which is proportional to the number of platelets aggregated. Such a consumption of these factors does not appear to occur after aggregation by other agents. Factor VIII procoagulant activity does not appear necessary for ristocetin-induced platelet aggregation, yet is utilized in this process. These findings support the hypothesis that the molecule associated with Factor VIII procoagulant activity is carried by the molecule necessary for ristocetin-induced platelet aggregation."}
{"id": "PMID:1087118", "title": "Post-transfusion thrombocytopenic purpura: a case report.", "content": "Post-transfusion thrombocytopenic purpura (PTTP) is a rare syndrome which was first brought to widespread attention by Shulman et al. Eighteen cases have been reported. The present report describes an additional patient, a multiparous female who developed petechia 7 days after blood transfusion. The patient's serum contained anti-P1A1 antibodies and her own platelets were P1A1-negative. She was treated with prednisone and recovered spontaneously without exchange transfusion of whole blood or plasma.", "contents": "Post-transfusion thrombocytopenic purpura: a case report. Post-transfusion thrombocytopenic purpura (PTTP) is a rare syndrome which was first brought to widespread attention by Shulman et al. Eighteen cases have been reported. The present report describes an additional patient, a multiparous female who developed petechia 7 days after blood transfusion. The patient's serum contained anti-P1A1 antibodies and her own platelets were P1A1-negative. She was treated with prednisone and recovered spontaneously without exchange transfusion of whole blood or plasma."}
{"id": "PMID:1087119", "title": "Traumatic endophthalmitis caused by an Erwinia species.", "content": "A previously healthy 70-year-old white man sustained a plant injury to his left eye while gardening and was admitted here because of an ensuing perforated cornea and endophthalmitis. The lens was opaque and its anterior capsule was ruptured. Culture of fluid obtained by anterior chamber paracentesis yielded a species of the Erwinia herbicolalathyri group (Enterobacter agglomerans). Thirty-seven days after a course of cefazolin and gentamicin therapy, corneal edema necessitated a planned extracapsular cataract extraction. The aqueous humor and lens again revealed the identical Erwinia species in pure culture. The patient received another course of cefazolin and showed improvement after the lens aspiration.", "contents": "Traumatic endophthalmitis caused by an Erwinia species. A previously healthy 70-year-old white man sustained a plant injury to his left eye while gardening and was admitted here because of an ensuing perforated cornea and endophthalmitis. The lens was opaque and its anterior capsule was ruptured. Culture of fluid obtained by anterior chamber paracentesis yielded a species of the Erwinia herbicolalathyri group (Enterobacter agglomerans). Thirty-seven days after a course of cefazolin and gentamicin therapy, corneal edema necessitated a planned extracapsular cataract extraction. The aqueous humor and lens again revealed the identical Erwinia species in pure culture. The patient received another course of cefazolin and showed improvement after the lens aspiration."}
{"id": "PMID:1087120", "title": "Ocular and cerebral involvement in familial lymphohistiocytosis.", "content": "A 5-month-old infant girl died of familial lymphohistiocytosis. The central nervous system showed widespread perivascular infiltration of the cerebral pia and arachnoid, the cerebral white matter and deep gray matter, the cerebellum, and brain stem by lymphocytes, benign appearing histiocytes, and macrophages with erythro-and lymphophagocytosis. The eyes had mild infiltration of the anterior uveal tract, moderate involvement of the inner retina, and marked infiltration of the optic nerves by identical cells.", "contents": "Ocular and cerebral involvement in familial lymphohistiocytosis. A 5-month-old infant girl died of familial lymphohistiocytosis. The central nervous system showed widespread perivascular infiltration of the cerebral pia and arachnoid, the cerebral white matter and deep gray matter, the cerebellum, and brain stem by lymphocytes, benign appearing histiocytes, and macrophages with erythro-and lymphophagocytosis. The eyes had mild infiltration of the anterior uveal tract, moderate involvement of the inner retina, and marked infiltration of the optic nerves by identical cells."}
{"id": "PMID:1087121", "title": "Orbital rim fat atrophy after repository periocular corticosteroid injection.", "content": "A 29-year-old white woman with acute recurrent nongranulomatous iridocyclitis of undetermined cause was treated with a retrobulbar injection of depocorticosteroid (Kenalog) through the inferior eyelid. This resulted in rapid clearing of the uveitis, but she developed subcutaneous and inferior orbital fat atrophy. This atrophy persisted during the 1 1/2 years of observation.", "contents": "Orbital rim fat atrophy after repository periocular corticosteroid injection. A 29-year-old white woman with acute recurrent nongranulomatous iridocyclitis of undetermined cause was treated with a retrobulbar injection of depocorticosteroid (Kenalog) through the inferior eyelid. This resulted in rapid clearing of the uveitis, but she developed subcutaneous and inferior orbital fat atrophy. This atrophy persisted during the 1 1/2 years of observation."}
{"id": "PMID:1087122", "title": "The acquisition of b-cell competence and diversity.", "content": "The in vitro splenic focus technique has been used to establish the characteristics of the repertoire of B cells in adult BALB/c mice, its stimulation and development. Evidence is presented that the technique yields an accurate estimate of the precursor cell frequency by providing monoclonal responses of a constant portion (4%) of transferred B cells. Since the monoclonally derived antibodies can be analyzed, it has also been possible to determine the isotype and clonotype of many of the resultant antibodies. The adult spleen can be shown to contain a vast repertoire (over 10(7) clonotypes) of primary B cells, each clonotype represented by from less than ten to as many as 10(4) responsive cells. Antigenic stimulation of these primary B cells is highly specific and apparently affinity dependent and leads to both antibody-forming cell clone formation and a population of secondary B cells. Secondary B cells, though derived from the same clonotypes, differ from primary B cell in several characteristics, including a lower degree of secificity of antigenic stimulation. In addition, secondary B cells can be stimulated in collaboration with allogeneic antigen-specific T cells to yield clones producing antibody of the IgG1 isotope, whereas primary B cells similarly stimulated produce only IgM antibodies. Analyses of neonatal B cells indicate that the acquisition of the adult repertoire is a highly patterned process. Thus, whereas 10(4) clonotypes are present at birth, more and more clonotypes are acquired in a highly ordered pattern during the first few weeks of life. It is concluded that this process is probably evolutionarily determined, since antigen-driven events appear to play no role. However, since developing B cells can be shown to be exquisitely sensitive to tolerance induction, negative antigenic selection may play an important role, particularly in eliminating clonotypes recognizing self-determinants for which the species is polymorphic.", "contents": "The acquisition of b-cell competence and diversity. The in vitro splenic focus technique has been used to establish the characteristics of the repertoire of B cells in adult BALB/c mice, its stimulation and development. Evidence is presented that the technique yields an accurate estimate of the precursor cell frequency by providing monoclonal responses of a constant portion (4%) of transferred B cells. Since the monoclonally derived antibodies can be analyzed, it has also been possible to determine the isotype and clonotype of many of the resultant antibodies. The adult spleen can be shown to contain a vast repertoire (over 10(7) clonotypes) of primary B cells, each clonotype represented by from less than ten to as many as 10(4) responsive cells. Antigenic stimulation of these primary B cells is highly specific and apparently affinity dependent and leads to both antibody-forming cell clone formation and a population of secondary B cells. Secondary B cells, though derived from the same clonotypes, differ from primary B cell in several characteristics, including a lower degree of secificity of antigenic stimulation. In addition, secondary B cells can be stimulated in collaboration with allogeneic antigen-specific T cells to yield clones producing antibody of the IgG1 isotope, whereas primary B cells similarly stimulated produce only IgM antibodies. Analyses of neonatal B cells indicate that the acquisition of the adult repertoire is a highly patterned process. Thus, whereas 10(4) clonotypes are present at birth, more and more clonotypes are acquired in a highly ordered pattern during the first few weeks of life. It is concluded that this process is probably evolutionarily determined, since antigen-driven events appear to play no role. However, since developing B cells can be shown to be exquisitely sensitive to tolerance induction, negative antigenic selection may play an important role, particularly in eliminating clonotypes recognizing self-determinants for which the species is polymorphic."}
{"id": "PMID:1087123", "title": "Pathogenesis of massively bleeding colonic diverticulosis: new observations.", "content": "Colonic diverticulosis is a common cause of acute severe rectal hemorrhage. The precise site of bleeding, etiology, and pathogenesis have not been previously identified. Arteriographic, microangiographic, and detailed histologic observations in 10 cases of massively bleeding colonic diverticulosis demonstrated strikingly consistent changes related to the characteristic angioarchitecture of colonic diverticula. These changes included: (1) asymmetric rupture of the vas rectum toward the lumen of the diverticulum precisely at its dome or its antimesenteric margin; (2) conspicuous eccentric intimal thickening of the vas rectum, often with thinning of the media and duplication of the internal elastic lamina at and near the bleeding point; and (3) general absence of diverticulitis. Comparison with control colonic diverticula suggests that traumatic factors arising within the diverticular or colonic lumen induce asymmetric intimal proliferation and segmental weakening of the associated vas rectum, predisposing to rupture and massive bleeding.", "contents": "Pathogenesis of massively bleeding colonic diverticulosis: new observations. Colonic diverticulosis is a common cause of acute severe rectal hemorrhage. The precise site of bleeding, etiology, and pathogenesis have not been previously identified. Arteriographic, microangiographic, and detailed histologic observations in 10 cases of massively bleeding colonic diverticulosis demonstrated strikingly consistent changes related to the characteristic angioarchitecture of colonic diverticula. These changes included: (1) asymmetric rupture of the vas rectum toward the lumen of the diverticulum precisely at its dome or its antimesenteric margin; (2) conspicuous eccentric intimal thickening of the vas rectum, often with thinning of the media and duplication of the internal elastic lamina at and near the bleeding point; and (3) general absence of diverticulitis. Comparison with control colonic diverticula suggests that traumatic factors arising within the diverticular or colonic lumen induce asymmetric intimal proliferation and segmental weakening of the associated vas rectum, predisposing to rupture and massive bleeding."}
{"id": "PMID:1087124", "title": "Prevention of upper gastrointestinal hemorrhage in 582 burned children.", "content": "In 582 burned children, neutralization of gastric acid and reduction of psychic stress were utilized to reduce upper gastrointestinal ulceration and hemorrhage. While receiving milk, diazepam, and psychologic support, two children required operation. Two of the children who died without clinically apparent gastrointestinal disease had ulcers discovered at autopsy. We conclude that a prophylactic regimen that reduced the amount of acid bathing the gastroduodenal mucosa, provides adequate calories, and minimizes psychic stress is useful in preventing gastrointestinal hemorrhage after burns.", "contents": "Prevention of upper gastrointestinal hemorrhage in 582 burned children. In 582 burned children, neutralization of gastric acid and reduction of psychic stress were utilized to reduce upper gastrointestinal ulceration and hemorrhage. While receiving milk, diazepam, and psychologic support, two children required operation. Two of the children who died without clinically apparent gastrointestinal disease had ulcers discovered at autopsy. We conclude that a prophylactic regimen that reduced the amount of acid bathing the gastroduodenal mucosa, provides adequate calories, and minimizes psychic stress is useful in preventing gastrointestinal hemorrhage after burns."}
{"id": "PMID:1087125", "title": "Immunologic status and lymphedema of the arm in postoperative patients with cancer of the breast.", "content": "Sixty women were evaluated regarding their immunologic status and for presence of lymphedema after surgery for breast carcinoma. Patients who had undergone radiotherapy were noted to have decreases in TLC, and increases in LIF. In addition, the patients also had an increased incidence of lymphedema after radical mastectomy.", "contents": "Immunologic status and lymphedema of the arm in postoperative patients with cancer of the breast. Sixty women were evaluated regarding their immunologic status and for presence of lymphedema after surgery for breast carcinoma. Patients who had undergone radiotherapy were noted to have decreases in TLC, and increases in LIF. In addition, the patients also had an increased incidence of lymphedema after radical mastectomy."}
{"id": "PMID:1087127", "title": "[Model and the experimental therapy of acute increase of intraocular pressure (author's transl)].", "content": "Comparative experiments were carried out on 63 rabbits to study the acute increase in intraocular pressure which develops in the eye after introduction of prostaglandin F2 (instillations, subconjunctival injections) and other substances (hypertonic solution of sodium chloride, and reopirin). Tonometry, tonography, and radioisotope studies of aqueous humor (24Na, albumin-labeled 131I) were done. Differences in the display and mechanisms of acute increase of intraocular pressure were marked following administration of prostaglandin and a hypertonic solution of sodium chloride. It is stated that prostaglandin increases the permeability of the blood-aqueous humor barrier for the 24Na ions in comparison with hypertonic solution of sodium chloride, and that the eye, under the influence of prostaglandin, heightens intraocular pressure. Pretreatment with dizinon (etamsilat) reduces the increase in intraocular pressure caused by prostaglandin.", "contents": "[Model and the experimental therapy of acute increase of intraocular pressure (author's transl)]. Comparative experiments were carried out on 63 rabbits to study the acute increase in intraocular pressure which develops in the eye after introduction of prostaglandin F2 (instillations, subconjunctival injections) and other substances (hypertonic solution of sodium chloride, and reopirin). Tonometry, tonography, and radioisotope studies of aqueous humor (24Na, albumin-labeled 131I) were done. Differences in the display and mechanisms of acute increase of intraocular pressure were marked following administration of prostaglandin and a hypertonic solution of sodium chloride. It is stated that prostaglandin increases the permeability of the blood-aqueous humor barrier for the 24Na ions in comparison with hypertonic solution of sodium chloride, and that the eye, under the influence of prostaglandin, heightens intraocular pressure. Pretreatment with dizinon (etamsilat) reduces the increase in intraocular pressure caused by prostaglandin."}
{"id": "PMID:1087128", "title": "Experimental suture studies in strabismus surgery. I. Reattachment rate of extraocular muscles after recession and resection operations.", "content": "We described a method for measuring the in vivo tensile strength of the muscle-scleral junction in rabbits. Data obtained on the reattachment time of extraocular muscles after recession and resection operations indicate the rate of healing is similar for both operations. Adequate wound healing is present by the 5th day after surgery, and sutures are probably unnecessary after 8 to 9 days. An ideal suture for strabismus surgery would be one that retains its strength during the first 8 to 9 days following surgery, then undergoes rapid absorption.", "contents": "Experimental suture studies in strabismus surgery. I. Reattachment rate of extraocular muscles after recession and resection operations. We described a method for measuring the in vivo tensile strength of the muscle-scleral junction in rabbits. Data obtained on the reattachment time of extraocular muscles after recession and resection operations indicate the rate of healing is similar for both operations. Adequate wound healing is present by the 5th day after surgery, and sutures are probably unnecessary after 8 to 9 days. An ideal suture for strabismus surgery would be one that retains its strength during the first 8 to 9 days following surgery, then undergoes rapid absorption."}
{"id": "PMID:1087129", "title": "Experimental suture studies in strabismus surgery. II. Comparison of tensile strength of plain catgut with polyglycolic acid (Dexon) sutures after extraocular muscle surgery.", "content": "From our experimental studies it appears Dexon satisfies more of the criteria for an ideal suture for strabismus surgery than plain catgut for the following reasons. Its handling, tying, and knot-holding (if tighthened firmly) qualities are excellent. Tissue reaction is minimal. It seems to be non-antigenic. Of paramount importance. Dexon's tensile strength is high initially and it retains its strength through the critical period of muscle-scleral wound healing, then dissolves in a rapid uniform manner.", "contents": "Experimental suture studies in strabismus surgery. II. Comparison of tensile strength of plain catgut with polyglycolic acid (Dexon) sutures after extraocular muscle surgery. From our experimental studies it appears Dexon satisfies more of the criteria for an ideal suture for strabismus surgery than plain catgut for the following reasons. Its handling, tying, and knot-holding (if tighthened firmly) qualities are excellent. Tissue reaction is minimal. It seems to be non-antigenic. Of paramount importance. Dexon's tensile strength is high initially and it retains its strength through the critical period of muscle-scleral wound healing, then dissolves in a rapid uniform manner."}
{"id": "PMID:1087130", "title": "[Findings on retinal surface by scanning electron microscopy. I. Retinal holes (author's transl)].", "content": "The findings by scanning electron microscopy on three retinal holes are described. An outgrow of a glial membrane with microvilli from the holes to the retinal surface can be seen. This epiretinal cell membrane grows on a felt of collagen fibres of the vitreoretinal borderlayer. Rudiments of visual receptors project from the deep walls into the holes. The layer of the pigment epithelial cells beneath the holes is remarkable by the abundance of microvilli.", "contents": "[Findings on retinal surface by scanning electron microscopy. I. Retinal holes (author's transl)]. The findings by scanning electron microscopy on three retinal holes are described. An outgrow of a glial membrane with microvilli from the holes to the retinal surface can be seen. This epiretinal cell membrane grows on a felt of collagen fibres of the vitreoretinal borderlayer. Rudiments of visual receptors project from the deep walls into the holes. The layer of the pigment epithelial cells beneath the holes is remarkable by the abundance of microvilli."}
{"id": "PMID:1087131", "title": "Kuhnt intermediary tissue as a barrier between the optic nerve and retina.", "content": "Utilizing adult albino rats and lanthanum nitrate as a tracer, electron microscopic studies were done to provide additional information on the blood-optic nerve barrier. Following injection into the cervical artery, lanthanum was observed to fill the intercellular space of the optic nerve parenchyma. Diffusion from the prelaminar optic nerve into the juxta-optic nerve retina was, however, prevented by the Kuhnt tissue at the level of the rod and cone layer. In this region, tight junctions were found between the glial cells of the Kuhnt intermediary tissue and were continuous from the tight junctions of the retinal pigment epithelial cells to the junctions (zonulae adhaerentes) of the outer limiting membrane. From the level of the outer limiting membrane to the inner limiting membrane there was no diffusion of lanthanum into the adjacent retina despite the absence of tight junctions, and the lanthanum which had diffused from the choroid was never observed in the area of the inner limiting membrane of the optic nerve head. It is in this region that the functional barrier may exist.", "contents": "Kuhnt intermediary tissue as a barrier between the optic nerve and retina. Utilizing adult albino rats and lanthanum nitrate as a tracer, electron microscopic studies were done to provide additional information on the blood-optic nerve barrier. Following injection into the cervical artery, lanthanum was observed to fill the intercellular space of the optic nerve parenchyma. Diffusion from the prelaminar optic nerve into the juxta-optic nerve retina was, however, prevented by the Kuhnt tissue at the level of the rod and cone layer. In this region, tight junctions were found between the glial cells of the Kuhnt intermediary tissue and were continuous from the tight junctions of the retinal pigment epithelial cells to the junctions (zonulae adhaerentes) of the outer limiting membrane. From the level of the outer limiting membrane to the inner limiting membrane there was no diffusion of lanthanum into the adjacent retina despite the absence of tight junctions, and the lanthanum which had diffused from the choroid was never observed in the area of the inner limiting membrane of the optic nerve head. It is in this region that the functional barrier may exist."}
{"id": "PMID:1087132", "title": "Ring-shaped acid burns of the rabbit cornea.", "content": "In the extension of our previous work on alkali burns of the rabbit cornea we have studied acid burns of the rabbit cornea using a ring-shaped chromoatographic paper soaked with solutions of different kinds of acids. The ensuing changes were studied with the silver methods of McGovern and Jabonero.", "contents": "Ring-shaped acid burns of the rabbit cornea. In the extension of our previous work on alkali burns of the rabbit cornea we have studied acid burns of the rabbit cornea using a ring-shaped chromoatographic paper soaked with solutions of different kinds of acids. The ensuing changes were studied with the silver methods of McGovern and Jabonero."}
{"id": "PMID:1087135", "title": "Insulin resistance and acanthosis nigricans. Report of a case with antibodies to insulin receptors.", "content": "A 64-year-old black man presented with the syndrome of acanthosis nigricans and insulin-resistant diabetes mellitus requiring up to 3000 units of insulin per day. The patient's plasma contained circulating antibodies to insulin receptors thought to be responsible for the insulin resistance. The marked insulin resistance, the manifestations of acanthosis nigricans, the evidence of immunologic dysfunction by the absence of expected circulating antibodies to insulin, and the demonstration of circulating  antibodies to insulin receptors put this patient in Kahn's category B of insulin resistance and acanthosis nigricans. There was no evidence of malignancy, lipodystrophy, or endocrine abnormality. The occurrence of acanthosis nigricans with insulin resistance due to binding of cell membrane insulin receptors by antibodies has been reported exclusively in women. This case report is the first description of a male patient with the syndrome of insulin resistance and acanthosis nigricans and focuses attention on features that might mislead one to suspect other causes of insulin resistance.", "contents": "Insulin resistance and acanthosis nigricans. Report of a case with antibodies to insulin receptors. A 64-year-old black man presented with the syndrome of acanthosis nigricans and insulin-resistant diabetes mellitus requiring up to 3000 units of insulin per day. The patient's plasma contained circulating antibodies to insulin receptors thought to be responsible for the insulin resistance. The marked insulin resistance, the manifestations of acanthosis nigricans, the evidence of immunologic dysfunction by the absence of expected circulating antibodies to insulin, and the demonstration of circulating  antibodies to insulin receptors put this patient in Kahn's category B of insulin resistance and acanthosis nigricans. There was no evidence of malignancy, lipodystrophy, or endocrine abnormality. The occurrence of acanthosis nigricans with insulin resistance due to binding of cell membrane insulin receptors by antibodies has been reported exclusively in women. This case report is the first description of a male patient with the syndrome of insulin resistance and acanthosis nigricans and focuses attention on features that might mislead one to suspect other causes of insulin resistance."}
{"id": "PMID:1087136", "title": "Involutional osteopenia: current concepts.", "content": "Involutional osteopenia is the most common metabolic bone disorder affecting the elderly population. Improved diagnostic techniques now allow for earlier detection of a decrease in bone mass than is possible with routine skeletal roentgenograms. Methods developed for quantitating bone remodeling have given new insight to the mechanisms involved in age-related skeletal loss. Theories of pathogenesis include [1] inadequate skeletal acquisition during youth, [2] dietary deficiencies of calcium and vitamin D, [3] hormonal imbalance, and [4] local factors influencing skeletal remodeling, particularly at the endosteal envelope. Numerous agents affecting bone remodeling have been used to treat involutional osteopenia, but none has been proved to restore bone mass. New programs involving combinations of agents give promise for increasing bone mineral content and, it is hoped, reducing fracture rate.", "contents": "Involutional osteopenia: current concepts. Involutional osteopenia is the most common metabolic bone disorder affecting the elderly population. Improved diagnostic techniques now allow for earlier detection of a decrease in bone mass than is possible with routine skeletal roentgenograms. Methods developed for quantitating bone remodeling have given new insight to the mechanisms involved in age-related skeletal loss. Theories of pathogenesis include [1] inadequate skeletal acquisition during youth, [2] dietary deficiencies of calcium and vitamin D, [3] hormonal imbalance, and [4] local factors influencing skeletal remodeling, particularly at the endosteal envelope. Numerous agents affecting bone remodeling have been used to treat involutional osteopenia, but none has been proved to restore bone mass. New programs involving combinations of agents give promise for increasing bone mineral content and, it is hoped, reducing fracture rate."}
{"id": "PMID:1087137", "title": "Immunochemotherapy of lung cancer.", "content": "After surgical resection of their primary lung cancer, 33 patients were randomized into one of three groups. The first received high-dose methotrexate once per month with citrovorum rescue, for 3 months. The second group were immunized monthly with a homogenate of Freund's complete adjuvant and carefully characterized soluble antigen derived from allogeneic lung cancer cells of appropriate histology, for 3 months. The third group received a combination of methotrexate and immunization monthly, for 3 months. Each patient was monitored immunologically before, during, and after the treatment period, by use of delayed hypersensitivity reactions to recall and cancer antigens, in vitro lymphocyte response to mitogens, and mixed lymphocyte blocking factor activity. The group that received methotrexate showed little change in skin reactivity, a reduction of blocking factor activity, and significant rebound overshoot in in vitro lymphocyte performance. The immunized group showed a tendency to production of blocking factor activity, striking conversion and enhancement of skin reactivity, and little change in in vitro lymphocyte performance. The immunochemotherapy group showed dramatic increases in specific skin reactivity to cancer antigens, up to 2 years after treatment, in vitro lymphocyte rebound overshoot, and reduction of blocking factor activity production. Classic life table analysis of the probability of freedom from metastases in patients with stage-I cancer indicate that the disease-free interval in patients who received methotrexate is longer than in historic and concomitant controls but not as long as in those who received immunization. The best group appear to be those who received combination immunochemotherapy. We emphasize that the small numbers in this pilot study do not yet allow firm conclusions to be made.", "contents": "Immunochemotherapy of lung cancer. After surgical resection of their primary lung cancer, 33 patients were randomized into one of three groups. The first received high-dose methotrexate once per month with citrovorum rescue, for 3 months. The second group were immunized monthly with a homogenate of Freund's complete adjuvant and carefully characterized soluble antigen derived from allogeneic lung cancer cells of appropriate histology, for 3 months. The third group received a combination of methotrexate and immunization monthly, for 3 months. Each patient was monitored immunologically before, during, and after the treatment period, by use of delayed hypersensitivity reactions to recall and cancer antigens, in vitro lymphocyte response to mitogens, and mixed lymphocyte blocking factor activity. The group that received methotrexate showed little change in skin reactivity, a reduction of blocking factor activity, and significant rebound overshoot in in vitro lymphocyte performance. The immunized group showed a tendency to production of blocking factor activity, striking conversion and enhancement of skin reactivity, and little change in in vitro lymphocyte performance. The immunochemotherapy group showed dramatic increases in specific skin reactivity to cancer antigens, up to 2 years after treatment, in vitro lymphocyte rebound overshoot, and reduction of blocking factor activity production. Classic life table analysis of the probability of freedom from metastases in patients with stage-I cancer indicate that the disease-free interval in patients who received methotrexate is longer than in historic and concomitant controls but not as long as in those who received immunization. The best group appear to be those who received combination immunochemotherapy. We emphasize that the small numbers in this pilot study do not yet allow firm conclusions to be made."}
{"id": "PMID:1087138", "title": "Transfer factor versus combination chemotherapy: a preliminary report of a randomized postsurgical adjuvant treatment study in osteogenic sarcoma.", "content": "Twenty-six patients with classic osteosarcoma were randomized to receive either transfer factor or combination chemotherapy. Eight of 14 patients who received transfer factor converted their skin test markers, evidence of activity of the transfer factor. Of these eight patients, all are alive; four are free of disease. Of the 18 patients who received combination chemotherapy, 14 are alive, 12 of whom are free of disease. The immunologic test procedures performed sequentially reveal that transfer factor appears to enhance cell-mediated immunity, but it is evident that in this study, a control (saline) arm in the protocol could not be included. It is of interest that the chemotherapy regimen used does not appear to suppress such activity permanently. The individual test results, however, are not very helpful for predicting response to treatment. The small numbers of patients and the short duration of this study, combined with the exclusion of parosteal osteogenic sarcomas and jaw tumors, do not permit a meaningful comparison with other published studies.", "contents": "Transfer factor versus combination chemotherapy: a preliminary report of a randomized postsurgical adjuvant treatment study in osteogenic sarcoma. Twenty-six patients with classic osteosarcoma were randomized to receive either transfer factor or combination chemotherapy. Eight of 14 patients who received transfer factor converted their skin test markers, evidence of activity of the transfer factor. Of these eight patients, all are alive; four are free of disease. Of the 18 patients who received combination chemotherapy, 14 are alive, 12 of whom are free of disease. The immunologic test procedures performed sequentially reveal that transfer factor appears to enhance cell-mediated immunity, but it is evident that in this study, a control (saline) arm in the protocol could not be included. It is of interest that the chemotherapy regimen used does not appear to suppress such activity permanently. The individual test results, however, are not very helpful for predicting response to treatment. The small numbers of patients and the short duration of this study, combined with the exclusion of parosteal osteogenic sarcomas and jaw tumors, do not permit a meaningful comparison with other published studies."}
{"id": "PMID:1087134", "title": "Cerebrospinal fluid pressure during O2 encephalography and N2O inhalation.", "content": "Cerebrospinal fluid (CSF) pressure was studied in 8 patients and 5 dogs during pneumoencephalography (PEG) or ventriculography in which either O2 or N2O was used as the contrast gas prior to and during N2O inhalation. In 7 patients, the use of O2 as the contrast gas increased CSF pressure 8.7 torr (range 4 to 12 torr) following N2O inhalation. In 1 patient, when N2O was used as the contrast gas, CSF pressure did not change after N2O inhalation. These findings were confirmed in anesthetized animals ventilated at a constant PaCO2. The authors conclude that if N2O inhalation is required during PEG, maximum patient safety can be achieved if the contrast gas is also N2O.", "contents": "Cerebrospinal fluid pressure during O2 encephalography and N2O inhalation. Cerebrospinal fluid (CSF) pressure was studied in 8 patients and 5 dogs during pneumoencephalography (PEG) or ventriculography in which either O2 or N2O was used as the contrast gas prior to and during N2O inhalation. In 7 patients, the use of O2 as the contrast gas increased CSF pressure 8.7 torr (range 4 to 12 torr) following N2O inhalation. In 1 patient, when N2O was used as the contrast gas, CSF pressure did not change after N2O inhalation. These findings were confirmed in anesthetized animals ventilated at a constant PaCO2. The authors conclude that if N2O inhalation is required during PEG, maximum patient safety can be achieved if the contrast gas is also N2O."}
{"id": "PMID:1087142", "title": "Long-term follow-up of sequential aortocoronary venous grafts.", "content": "In 1972 we reported myocardial revascularization of 130 patients using multiple sequential aortocoronary anastomoses to a single saphenous vein ]1]. Of the 122 survivors described in that report, 121 (99%) have been followed an additional 3 years. Twelve deaths occurred during the interval. The 110 currently followed patients represent 290 anastomoses; 54 have been studied angiographically since operation. Angiographic patency at 3 years in the studied group (18) was 70%. These figures exceed our follow-up data for 135 patients revascularized during the same period using individual vein grafts. Comparison of life table survival curves demonstrates this result. We believe the improved patency and decreased operating time that have resulted from employing this technique have outweighed the likelihood of a proximal stenosis causing closure of the whole graft system. We continue to use this technique in combination with internal mammary artery grafts in the management of multiple-vessel coronary disease. Good early results using this technique have been reported by other authors [2, 3, 5].", "contents": "Long-term follow-up of sequential aortocoronary venous grafts. In 1972 we reported myocardial revascularization of 130 patients using multiple sequential aortocoronary anastomoses to a single saphenous vein ]1]. Of the 122 survivors described in that report, 121 (99%) have been followed an additional 3 years. Twelve deaths occurred during the interval. The 110 currently followed patients represent 290 anastomoses; 54 have been studied angiographically since operation. Angiographic patency at 3 years in the studied group (18) was 70%. These figures exceed our follow-up data for 135 patients revascularized during the same period using individual vein grafts. Comparison of life table survival curves demonstrates this result. We believe the improved patency and decreased operating time that have resulted from employing this technique have outweighed the likelihood of a proximal stenosis causing closure of the whole graft system. We continue to use this technique in combination with internal mammary artery grafts in the management of multiple-vessel coronary disease. Good early results using this technique have been reported by other authors [2, 3, 5]."}
{"id": "PMID:1087143", "title": "Why do radial artery grafts for aortocoronary bypass fail? A reappraisal.", "content": "The reasons for the high failure rate of radial artery grafts for aortocoronary bypass (ACB) are appraised. Each of 11 dogs received four types of grafts: femoral arteries were grafted to carotid arteries, and jugular veins were grafted to femoral arteries bilaterally. One of each pair of grafts was wrapped in a plastic sheet in order to interfere with the regeneration of vasa vasorum. The grafts were examined 2 months later. The results suggest that although operative trauma and intraluminal pressure change play important roles, the thick-walled artery appears to depend more on vasa vasorum for its integrity than does the thin-walled vein. A free radical artery graft, with vasa vasorum disrupted at both ends, cannot regenerate readily from the adjacent tissue, as is the case at the ACB position, and is therefore more vulnerable to subintimal hyperplasia and occlusion than either the vein graft or the internal mammary artery graft; both of these grafts are thinner, and the internal mammary artery graft remains undisrupted at one end.", "contents": "Why do radial artery grafts for aortocoronary bypass fail? A reappraisal. The reasons for the high failure rate of radial artery grafts for aortocoronary bypass (ACB) are appraised. Each of 11 dogs received four types of grafts: femoral arteries were grafted to carotid arteries, and jugular veins were grafted to femoral arteries bilaterally. One of each pair of grafts was wrapped in a plastic sheet in order to interfere with the regeneration of vasa vasorum. The grafts were examined 2 months later. The results suggest that although operative trauma and intraluminal pressure change play important roles, the thick-walled artery appears to depend more on vasa vasorum for its integrity than does the thin-walled vein. A free radical artery graft, with vasa vasorum disrupted at both ends, cannot regenerate readily from the adjacent tissue, as is the case at the ACB position, and is therefore more vulnerable to subintimal hyperplasia and occlusion than either the vein graft or the internal mammary artery graft; both of these grafts are thinner, and the internal mammary artery graft remains undisrupted at one end."}
{"id": "PMID:1087144", "title": "Coronary artery bypass occlusion second to postcardiotomy syndrome.", "content": "Postcardiotomy syndrome following coronary vein bypass procedures may cause graft occlusion as a result of the associated myxedematous hyperemic inflammation that later forms dense obliterative fibrosis. Variability of symptoms and onset make early recognition of postcardiotomy syndrome difficult. However, the presence of the classic triad of fever, chest pain, and pleuroericardial reactions along with leukocytosis and elevated sedimentation rate should suggest the diagnosis. Early recognition and prompt institution of steroid therapy offer relief of symptoms and regression of inflammation and probably reduce the incidence of graft occlusion. From early experience with 14 patients who developed postcardiotomy syndrome and received only symptomatic treatment, 12 developed graft occlusion, whereas in 31 subsequent patients with this syndrome who were treated with steroids, only 5 demonstrated graft occlusion.", "contents": "Coronary artery bypass occlusion second to postcardiotomy syndrome. Postcardiotomy syndrome following coronary vein bypass procedures may cause graft occlusion as a result of the associated myxedematous hyperemic inflammation that later forms dense obliterative fibrosis. Variability of symptoms and onset make early recognition of postcardiotomy syndrome difficult. However, the presence of the classic triad of fever, chest pain, and pleuroericardial reactions along with leukocytosis and elevated sedimentation rate should suggest the diagnosis. Early recognition and prompt institution of steroid therapy offer relief of symptoms and regression of inflammation and probably reduce the incidence of graft occlusion. From early experience with 14 patients who developed postcardiotomy syndrome and received only symptomatic treatment, 12 developed graft occlusion, whereas in 31 subsequent patients with this syndrome who were treated with steroids, only 5 demonstrated graft occlusion."}
{"id": "PMID:1087146", "title": "T and B lymphocytes in patients with pulmonary tuberculosis.", "content": "Counts of T and B lymphocytes were made in the peripheral blood from patients with active and inactive pulmonary tuberculosis and in healthy subjects. In patients with active pulmonary tuberculosis the numbers of T cells were significantly lower than in the controls. After treatment for two months, if the patient's condition improved the percentage of T cells returned to nearly normal levels. If the patient failed to improve, the numbers of T cells remained low. The mechanism of these phenomena is discussed.", "contents": "T and B lymphocytes in patients with pulmonary tuberculosis. Counts of T and B lymphocytes were made in the peripheral blood from patients with active and inactive pulmonary tuberculosis and in healthy subjects. In patients with active pulmonary tuberculosis the numbers of T cells were significantly lower than in the controls. After treatment for two months, if the patient's condition improved the percentage of T cells returned to nearly normal levels. If the patient failed to improve, the numbers of T cells remained low. The mechanism of these phenomena is discussed."}
{"id": "PMID:1087147", "title": "[Role of duration and intensity of activation of afferent fibers in regulating morphologic shifts in a neuron].", "content": "The afferent impulsation has a regulating influence on morphological and histochemical shifts in the neuron. The size and staining of spinal motoneurons of the frog are changed depending on the force and duration of the applied high frequency stimulation of afferent fibres. The threshold 10-minute-long activation results in the increased size and lighter staining of nerve cells. An increased force of high frequency stimuli causes darker staining and a decreased size of the motoneurons under study. One-minute-long activation of the afferent fibres by electric stimuli of 50 imp/sec failed to cause changes in the size and staining of nerve cells. Prolongation of the activation till 10 minutes results in a sharp decrease of the neuron size. Further activation of the afferent fibres for 2 hours causes pathological shifts in the structure of motoneurons: their vacuolization and melting. Besides, there appears a great number of dark stretched intensively stained nerve cells.", "contents": "[Role of duration and intensity of activation of afferent fibers in regulating morphologic shifts in a neuron]. The afferent impulsation has a regulating influence on morphological and histochemical shifts in the neuron. The size and staining of spinal motoneurons of the frog are changed depending on the force and duration of the applied high frequency stimulation of afferent fibres. The threshold 10-minute-long activation results in the increased size and lighter staining of nerve cells. An increased force of high frequency stimuli causes darker staining and a decreased size of the motoneurons under study. One-minute-long activation of the afferent fibres by electric stimuli of 50 imp/sec failed to cause changes in the size and staining of nerve cells. Prolongation of the activation till 10 minutes results in a sharp decrease of the neuron size. Further activation of the afferent fibres for 2 hours causes pathological shifts in the structure of motoneurons: their vacuolization and melting. Besides, there appears a great number of dark stretched intensively stained nerve cells."}
{"id": "PMID:1087148", "title": "Vitrectomy in endophthalmitis. Results of study using vitrectomy, intraocular antibiotics, or a combination of both.", "content": "A rabbit model of endophthalmitis was produced by inoculating Staphylococcus epidermidis and S aureus into the vitreous cavity. Elimination of microorganisms was compared using intravitreal administration of 0.1 mg of gentamicin alone, vitrectomy alone, and a combination of gentamicin and vitrectomy. In the case of S. epidermidis treated 24 hours after inoculation, all untreated eyes were culture-positive at one week, eyes treated with gentamicin alone or intravitreal gentamicin in combination with vitrectomy were all culture-negative, and vitrectomy alone rendered half of the eyes culture-negative. In the case of S aureus, eyes not treated by vitrectomy alone were all culture-positive at one week. Eyes treated with intravitreal gentamicin 25 to 31 hours after infection were culture-negative for S aireus in 33% at one week, while eyes treated with combined vitrectomy plus intraocular gentamicin were culture-negative in 83% of cases. When treatment was delayed 40 to 49 hours after inoculation of S aureus, intravitreal rendered 50% culture-negative at one week, while vitrectomy combined with intravitreal gentamicin eliminated the infection in 100% of eyes.", "contents": "Vitrectomy in endophthalmitis. Results of study using vitrectomy, intraocular antibiotics, or a combination of both. A rabbit model of endophthalmitis was produced by inoculating Staphylococcus epidermidis and S aureus into the vitreous cavity. Elimination of microorganisms was compared using intravitreal administration of 0.1 mg of gentamicin alone, vitrectomy alone, and a combination of gentamicin and vitrectomy. In the case of S. epidermidis treated 24 hours after inoculation, all untreated eyes were culture-positive at one week, eyes treated with gentamicin alone or intravitreal gentamicin in combination with vitrectomy were all culture-negative, and vitrectomy alone rendered half of the eyes culture-negative. In the case of S aureus, eyes not treated by vitrectomy alone were all culture-positive at one week. Eyes treated with intravitreal gentamicin 25 to 31 hours after infection were culture-negative for S aireus in 33% at one week, while eyes treated with combined vitrectomy plus intraocular gentamicin were culture-negative in 83% of cases. When treatment was delayed 40 to 49 hours after inoculation of S aureus, intravitreal rendered 50% culture-negative at one week, while vitrectomy combined with intravitreal gentamicin eliminated the infection in 100% of eyes."}
{"id": "PMID:1087149", "title": "Cerebellopontine pantopaque cisternography and tomography of the internal acoustic meatus in the diagnosis of angle tumors.", "content": "The results of 106 consecutive Pantopaque cisternographies performed at the neuro-radiological department, Rigshospitalet, are presented. 41 positive and 65 negative examinations were found. Good accordance between radiological tumor size estimation and size of actual, surgically verified tumor was noted. A comparison between tomography of the internal auditory canal and pantopaque cisternography of the posterior fossa has been made, and we found 6 cases with normal tomography and positive cisternography. Furthermore 17 cases (26%) with pathological tomographic examination of internal auditory canal as well as a negative finding at Pantopaque cisternography, were encountered. Therefore we conclude that asymmetry of the radiological appearance of the internal auditory canal at tomography is not of decisive importance in the indication for cisternography, but the examination should be included in the test battery in search for cerebellopontine angle pathology. We agree with other authors that Pantopaque cisternography is without any doubt the final and most conclusive examination available for the diagnosis of cerebellopontine angle tumors. A system of grading the different objective findings giving indication for Pantopaque cisternography has been made.", "contents": "Cerebellopontine pantopaque cisternography and tomography of the internal acoustic meatus in the diagnosis of angle tumors. The results of 106 consecutive Pantopaque cisternographies performed at the neuro-radiological department, Rigshospitalet, are presented. 41 positive and 65 negative examinations were found. Good accordance between radiological tumor size estimation and size of actual, surgically verified tumor was noted. A comparison between tomography of the internal auditory canal and pantopaque cisternography of the posterior fossa has been made, and we found 6 cases with normal tomography and positive cisternography. Furthermore 17 cases (26%) with pathological tomographic examination of internal auditory canal as well as a negative finding at Pantopaque cisternography, were encountered. Therefore we conclude that asymmetry of the radiological appearance of the internal auditory canal at tomography is not of decisive importance in the indication for cisternography, but the examination should be included in the test battery in search for cerebellopontine angle pathology. We agree with other authors that Pantopaque cisternography is without any doubt the final and most conclusive examination available for the diagnosis of cerebellopontine angle tumors. A system of grading the different objective findings giving indication for Pantopaque cisternography has been made."}
{"id": "PMID:1087150", "title": "[The activity of motor units of the musculus splenius capitis in pheripheral vestibular lesions (author's transl)].", "content": "The activity of single motor units of the splenius-capitis muscle of healthy human subjects and patients with unilateral vestibular lesions was recorded by means of microelectrodes. The major part of these units reacted to vestibular stimulation in the same way as primary and secondary vestibular neurones of other vertebrates. The reactions of these units to trapezoidale stimuli is identical to that of vestibular neurones. An angular acceleration of more than 0.6 degrees/s2 is for these units a stimulus which is above threshold, this too corresponds to findings in the vestibular nerve. In patients with unilateral vestibular lesions the threshold for rotatory stimuli is markedly increased on the side of the lesion, whereas the threshold on the other side remains constant. This method is very sensitive and vestibular lesions can be detected which cannot be seen using the common technics for vestibular examination.", "contents": "[The activity of motor units of the musculus splenius capitis in pheripheral vestibular lesions (author's transl)]. The activity of single motor units of the splenius-capitis muscle of healthy human subjects and patients with unilateral vestibular lesions was recorded by means of microelectrodes. The major part of these units reacted to vestibular stimulation in the same way as primary and secondary vestibular neurones of other vertebrates. The reactions of these units to trapezoidale stimuli is identical to that of vestibular neurones. An angular acceleration of more than 0.6 degrees/s2 is for these units a stimulus which is above threshold, this too corresponds to findings in the vestibular nerve. In patients with unilateral vestibular lesions the threshold for rotatory stimuli is markedly increased on the side of the lesion, whereas the threshold on the other side remains constant. This method is very sensitive and vestibular lesions can be detected which cannot be seen using the common technics for vestibular examination."}
{"id": "PMID:1087151", "title": "Factors affecting responses to immunization.", "content": "Geoffrey Edsall once stated \"...at its best, immunization is the cheapest and most practical means of controlling infectious disease...\". While immunization against some diseases has been highly successful, the efficacy of some vaccines is far from adequate. Basic immunological research has identified many factors which control the development and operation of the immune system of vertebrate animals; application of this information to the development and practical use of vaccines for humans may eventually benefit this general approach to the control of infectious disease.", "contents": "Factors affecting responses to immunization. Geoffrey Edsall once stated \"...at its best, immunization is the cheapest and most practical means of controlling infectious disease...\". While immunization against some diseases has been highly successful, the efficacy of some vaccines is far from adequate. Basic immunological research has identified many factors which control the development and operation of the immune system of vertebrate animals; application of this information to the development and practical use of vaccines for humans may eventually benefit this general approach to the control of infectious disease."}
{"id": "PMID:1087152", "title": "Immunological aspects of cancer.", "content": "Immunology occupies a central role in the area of cancer, both in research and clinical practice. This article first reviews normal immunity and describes the interaction of the cancer cell with cellular and humoral immune mechanisms. Such mechanisms play a considerable role in the emergence, proliferation, metastases, regression and recurrence of tumours. Immunological investigations are applicable to the diagnosis of some forms of cancer, especially leukaemias, lymphomas and myelomas as well as analysing the immune status of patients suffering from cancer, which give significant guidelines with respect to prognosis. Manipulation of immune response in the form of immunotherapy is emerging as an important area in cancer therapy.", "contents": "Immunological aspects of cancer. Immunology occupies a central role in the area of cancer, both in research and clinical practice. This article first reviews normal immunity and describes the interaction of the cancer cell with cellular and humoral immune mechanisms. Such mechanisms play a considerable role in the emergence, proliferation, metastases, regression and recurrence of tumours. Immunological investigations are applicable to the diagnosis of some forms of cancer, especially leukaemias, lymphomas and myelomas as well as analysing the immune status of patients suffering from cancer, which give significant guidelines with respect to prognosis. Manipulation of immune response in the form of immunotherapy is emerging as an important area in cancer therapy."}
{"id": "PMID:1087158", "title": "Stimulation of B-lymphocyte colony formation in vitro by sera from patients with leukaemia or lymphoma.", "content": "Studies were made on the effects of 665 sera, from normal donors or patients with various diseases, on B-lymphocyte colony formation in agar by mouse spleen cells. Undiluted serum from most normal donors inhibited colony formation, but 43-53% of sera from patients with histiocytic lymphoma, lymphocytic lymphoma or Hodgkin's disease stimulated colony formation, serum activity correlating with the stage of the disease. Moderate colony-stimulating activity was observed with serum taken from patients with acute lymphoid or myeloid leukaemia following, but not prior to, chemotherapy. Colony stimulating activity was not correlated with the blood group of serum donors and could not be ascribed to the presence of endotoxin, red cells or mouse red cell haemagglutinins in the active sera. Elevated colony stimulating activity was not observed in sera from patients with non-neoplastic disorders ot haemopoiesis or with diseases of other organ systems.", "contents": "Stimulation of B-lymphocyte colony formation in vitro by sera from patients with leukaemia or lymphoma. Studies were made on the effects of 665 sera, from normal donors or patients with various diseases, on B-lymphocyte colony formation in agar by mouse spleen cells. Undiluted serum from most normal donors inhibited colony formation, but 43-53% of sera from patients with histiocytic lymphoma, lymphocytic lymphoma or Hodgkin's disease stimulated colony formation, serum activity correlating with the stage of the disease. Moderate colony-stimulating activity was observed with serum taken from patients with acute lymphoid or myeloid leukaemia following, but not prior to, chemotherapy. Colony stimulating activity was not correlated with the blood group of serum donors and could not be ascribed to the presence of endotoxin, red cells or mouse red cell haemagglutinins in the active sera. Elevated colony stimulating activity was not observed in sera from patients with non-neoplastic disorders ot haemopoiesis or with diseases of other organ systems."}
{"id": "PMID:1087159", "title": "The fine structure of pulmonary contusion and the effect of various drugs.", "content": "The results of contusion were examined by electron and light microscopy in the lungs of rats. It was found that the results here were very similar to those elsewhere in the body, with a few minor modifications due to the unique structure of the lung. Densitometry of protein concentration and visual estimation of oedema were used to quantitate the effects on the injury. The benzo-pyrone drug Venalot had a considerable effect in reducing the protein concentration in the air spaces and the interstitial tissue, and of the oedema in the latter. Neither the proteinase inhibitor Trasylol nor the pectin-based plasma expander HAS had any significant effect on the fine structural alterations of pulmonary contusion.", "contents": "The fine structure of pulmonary contusion and the effect of various drugs. The results of contusion were examined by electron and light microscopy in the lungs of rats. It was found that the results here were very similar to those elsewhere in the body, with a few minor modifications due to the unique structure of the lung. Densitometry of protein concentration and visual estimation of oedema were used to quantitate the effects on the injury. The benzo-pyrone drug Venalot had a considerable effect in reducing the protein concentration in the air spaces and the interstitial tissue, and of the oedema in the latter. Neither the proteinase inhibitor Trasylol nor the pectin-based plasma expander HAS had any significant effect on the fine structural alterations of pulmonary contusion."}
{"id": "PMID:1087154", "title": "IgG antilymphocyte antibodies in SLE detected by 125I protein A.", "content": "125I-labeled protein A was utilized to detect lymphocyte binding of IgG at 37 degrees C in 50 sera from patients with SLE. IgG binding was partially inhibited by preincubation of test cells with immune complexes in 50% of sera. In 10%, cytophilic Ig was increased by immune complex preincubation, and in 40% no appreciable change was recorded. With purified preparations of T and B cells, binding of SLE IgG at 37 degrees C was equal for both cell populations.", "contents": "IgG antilymphocyte antibodies in SLE detected by 125I protein A. 125I-labeled protein A was utilized to detect lymphocyte binding of IgG at 37 degrees C in 50 sera from patients with SLE. IgG binding was partially inhibited by preincubation of test cells with immune complexes in 50% of sera. In 10%, cytophilic Ig was increased by immune complex preincubation, and in 40% no appreciable change was recorded. With purified preparations of T and B cells, binding of SLE IgG at 37 degrees C was equal for both cell populations."}
{"id": "PMID:1087155", "title": "Suppression of autoimmunity in NZB mice with steroid-sensitive X-radiation-sensitive syngeneic young thymocytes.", "content": "NZB mice spontaneously lose thymic regulatory or suppressor cells and subsequently develop autoimmunity and lymphoreticular hyperplasia. Treatment with 2-week-old syngeneic thymocytes every 2 weeks starting at 4 weeks of age suppressed these disease manifestations. The active thymocyte subpopulation was found to be sensitive to corticosteroids and X-irradiation.", "contents": "Suppression of autoimmunity in NZB mice with steroid-sensitive X-radiation-sensitive syngeneic young thymocytes. NZB mice spontaneously lose thymic regulatory or suppressor cells and subsequently develop autoimmunity and lymphoreticular hyperplasia. Treatment with 2-week-old syngeneic thymocytes every 2 weeks starting at 4 weeks of age suppressed these disease manifestations. The active thymocyte subpopulation was found to be sensitive to corticosteroids and X-irradiation."}
{"id": "PMID:1087160", "title": "Orientation of chromophores in reaction centers of Rhodopseudomonas sphaeroides. Evidence for two absorption bands of the dimeric primary electron donor.", "content": "Chromatophores from Rhodopseudomonas sphaeroides were oriented by allowing aqueous suspensions to dry on glass plates. Orientation of reaction center pigments was investigated by studying the linear dichroism of chromatophores in which the absorption by antenna bacteriochlorophyll had been attenuated through selective oxidation. Alternatively the light-induced absorbance changes, in the ranges 550-650 and 700-950nm, were studied in untreated chromatophores. The long wave transition moment of reaction center bacteriochlorophyll (P-870) was found to be nearly parallel to the plane of the membrane, whereas the long wave transition moments of bacteriopheophytin are polarized out of this plane. For light-induced changes the linear dichroic ratios, defined as deltaav/deltaah, are nearly the same for untreated and for oxidized chromatophores. Typical values are 1.60 at 870 nm, 0.80 at 810nm, 1.20 at 790 nm, 0.70 at 765 nm, 0.30 at 745 nm , and 0.50 at 600 nm. The different values for the absorbance decrease at 810 nm (0.80) and the increase at 790 nm (1.20) are incompatible with the hypothesis that these changes are due to the blue-shift of a single band. We propose that the decreases at 870 and 810 nm reflect bleaching of the two components of a bacteriochlorophyll dimer, the \"special pair\" that shares in the photochemical donation of a single electron. The increase at 790 nm then represents the appearance of a monomer band in place of the dimer spectrum, as a result of electron donation. This hypothesis is consistent with available data on circular dichroism. It is confirmed by the presence of a shoulder at 810 nm in the absorption spectrum of reaction centers at low temperature; this band disappears upon photooxidation of the reaction centers. For the changes near 760 nm, associated with bacteriopheophytin, the polarization and the shape of the \"light-dark\" difference spectrum (identical to the first derivative of the absorption spectrum) show that the 760 nm band undergoes a light-induced shift to greater wavelengths.", "contents": "Orientation of chromophores in reaction centers of Rhodopseudomonas sphaeroides. Evidence for two absorption bands of the dimeric primary electron donor. Chromatophores from Rhodopseudomonas sphaeroides were oriented by allowing aqueous suspensions to dry on glass plates. Orientation of reaction center pigments was investigated by studying the linear dichroism of chromatophores in which the absorption by antenna bacteriochlorophyll had been attenuated through selective oxidation. Alternatively the light-induced absorbance changes, in the ranges 550-650 and 700-950nm, were studied in untreated chromatophores. The long wave transition moment of reaction center bacteriochlorophyll (P-870) was found to be nearly parallel to the plane of the membrane, whereas the long wave transition moments of bacteriopheophytin are polarized out of this plane. For light-induced changes the linear dichroic ratios, defined as deltaav/deltaah, are nearly the same for untreated and for oxidized chromatophores. Typical values are 1.60 at 870 nm, 0.80 at 810nm, 1.20 at 790 nm, 0.70 at 765 nm, 0.30 at 745 nm , and 0.50 at 600 nm. The different values for the absorbance decrease at 810 nm (0.80) and the increase at 790 nm (1.20) are incompatible with the hypothesis that these changes are due to the blue-shift of a single band. We propose that the decreases at 870 and 810 nm reflect bleaching of the two components of a bacteriochlorophyll dimer, the \"special pair\" that shares in the photochemical donation of a single electron. The increase at 790 nm then represents the appearance of a monomer band in place of the dimer spectrum, as a result of electron donation. This hypothesis is consistent with available data on circular dichroism. It is confirmed by the presence of a shoulder at 810 nm in the absorption spectrum of reaction centers at low temperature; this band disappears upon photooxidation of the reaction centers. For the changes near 760 nm, associated with bacteriopheophytin, the polarization and the shape of the \"light-dark\" difference spectrum (identical to the first derivative of the absorption spectrum) show that the 760 nm band undergoes a light-induced shift to greater wavelengths."}
{"id": "PMID:1087156", "title": "Transfer of spleen cells from young to aging NZB X NZW F1 hybrid mice. Effect on mortality, antinuclear antibody, and renal disease.", "content": "Multiple injections of spleen cells from young NZB x NZW F1 hybrid mice with a mean age of 9 weeks were administered to syngeneic recipients at 10-day intervals beginning at 3 months of age. The recipient mice had a dealyed appearance of antinuclear antibody, decreased cumulative incidence of antinuclear antibody positivity up to 7.3 months of age, lower cumulative mortality up to 8 months of age, and a lesser degree of glomerular sclerosis at 8 months of age. By 9 months of age no significant differences in these parameters remained. Transferred cells were most effective during the 4- to 6-week period immediately following initiation of injections. The beneficial effects appeared to reflect an early but temporary suppression of the autoimmune process by the transferred lymphoid cells.", "contents": "Transfer of spleen cells from young to aging NZB X NZW F1 hybrid mice. Effect on mortality, antinuclear antibody, and renal disease. Multiple injections of spleen cells from young NZB x NZW F1 hybrid mice with a mean age of 9 weeks were administered to syngeneic recipients at 10-day intervals beginning at 3 months of age. The recipient mice had a dealyed appearance of antinuclear antibody, decreased cumulative incidence of antinuclear antibody positivity up to 7.3 months of age, lower cumulative mortality up to 8 months of age, and a lesser degree of glomerular sclerosis at 8 months of age. By 9 months of age no significant differences in these parameters remained. Transferred cells were most effective during the 4- to 6-week period immediately following initiation of injections. The beneficial effects appeared to reflect an early but temporary suppression of the autoimmune process by the transferred lymphoid cells."}
{"id": "PMID:1087162", "title": "The interaction of alpha-1-antitrypsin with soluble and sepharose-bound elastase.", "content": "The products resulting from the interaction of alpha-1-antitrypsin with elastase were examined with polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate, and by affinity chromatography. Five products of the reaction can be identified by polyacrylamide disc gel electrophoresis. Two products are complexes between alpha-1-antitrypsin and elastase (73 800 and 58 300 daltons). Two additional products are identical to fragments of alpha-1-antitrypsin which can be washed from a column of Sepharose-bound elastase immediately after alpha-1-antitrypsin is applied to the column. The larger component about 50 000 daltons, reacts with antiserum to alpha-1-antitrypsin, and does not inhibit enzymes. Together, these two products have an amino acid analysis similar to alpha-1-antitrypsin. These two fragments are probably hydrolytic products of the interaction of elastase with alpha-1-antitrypsin which is biologically inactive. The fifth product is probably a fragment of alpha-1-antitrypsin missing from the low molecular weight complex. The components of the complexes can be separated from each other by a mild nucleophilic attack. Small quantities of alpha-1-antitrypsin can be displaced from the elastase affinity column by phenyl methane sulfonyl fluoride. In conclusion, porcine pancreatic elastase forms two complexes with alpha-1-antitrypsin. One or both complexes can be split by alkali.", "contents": "The interaction of alpha-1-antitrypsin with soluble and sepharose-bound elastase. The products resulting from the interaction of alpha-1-antitrypsin with elastase were examined with polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate, and by affinity chromatography. Five products of the reaction can be identified by polyacrylamide disc gel electrophoresis. Two products are complexes between alpha-1-antitrypsin and elastase (73 800 and 58 300 daltons). Two additional products are identical to fragments of alpha-1-antitrypsin which can be washed from a column of Sepharose-bound elastase immediately after alpha-1-antitrypsin is applied to the column. The larger component about 50 000 daltons, reacts with antiserum to alpha-1-antitrypsin, and does not inhibit enzymes. Together, these two products have an amino acid analysis similar to alpha-1-antitrypsin. These two fragments are probably hydrolytic products of the interaction of elastase with alpha-1-antitrypsin which is biologically inactive. The fifth product is probably a fragment of alpha-1-antitrypsin missing from the low molecular weight complex. The components of the complexes can be separated from each other by a mild nucleophilic attack. Small quantities of alpha-1-antitrypsin can be displaced from the elastase affinity column by phenyl methane sulfonyl fluoride. In conclusion, porcine pancreatic elastase forms two complexes with alpha-1-antitrypsin. One or both complexes can be split by alkali."}
{"id": "PMID:1087163", "title": "Effect of furosemide on unidirectional fluxes of sodium and chloride across the skin of the frog, Rana pipiens.", "content": "1. The diuretic furosemide, when added to the outside solution at a concentration of 5-10-4 M, increases the electrical potential difference (PD) across the isolated frog skin, but the short-circuit current (Isc) is unchanged. Lower concentrations had no significant effect on these electrical parameters. 2. When SO42- or NO3- are substituted for Cl- in the Ringer's solution furosemide has no effect on the PD or Isc. 3. Simultaneous unidirectional fluxes of Na+ and Cl- show that furosemide (5-10-4 M outside) reduces both the influx and outflux of Cl-, while the Na+ fluxes are not altered. 4. Furosemide (5-10-4 M) on the corium side of the frog skin had no significant effect on either PD, Isc or undirectional fluxes of Cl-. 5. It is suggested that furosemide reduces passive Cl- transfer, possibly by interacting with the Cl-/Cl- exchange diffusion mechanism which has been observed in this tissue. These observations further suggest that perhaps the diuretic action of furosemide may be mediated by such an effect on passive Cl- permeability which is linked to the active Cl- transport mechanism in the renal tubule.", "contents": "Effect of furosemide on unidirectional fluxes of sodium and chloride across the skin of the frog, Rana pipiens. 1. The diuretic furosemide, when added to the outside solution at a concentration of 5-10-4 M, increases the electrical potential difference (PD) across the isolated frog skin, but the short-circuit current (Isc) is unchanged. Lower concentrations had no significant effect on these electrical parameters. 2. When SO42- or NO3- are substituted for Cl- in the Ringer's solution furosemide has no effect on the PD or Isc. 3. Simultaneous unidirectional fluxes of Na+ and Cl- show that furosemide (5-10-4 M outside) reduces both the influx and outflux of Cl-, while the Na+ fluxes are not altered. 4. Furosemide (5-10-4 M) on the corium side of the frog skin had no significant effect on either PD, Isc or undirectional fluxes of Cl-. 5. It is suggested that furosemide reduces passive Cl- transfer, possibly by interacting with the Cl-/Cl- exchange diffusion mechanism which has been observed in this tissue. These observations further suggest that perhaps the diuretic action of furosemide may be mediated by such an effect on passive Cl- permeability which is linked to the active Cl- transport mechanism in the renal tubule."}
{"id": "PMID:1087164", "title": "Analogy tests and psychopathology at follow-up after open heart surgery.", "content": "A study of 100 coronary bypass and cardiac valvular surgery patients investigated whether preoperative brain damage, as measured by the Conceptual Level Analogy Test (CLAT), is a major risk factor for postoperative psychiatric symptoms and mortality. Three cognitive psychological tests, including the CLAT, and psychatric interviews were given preoperatively, postoperatively, and at 18-month follow-up. Surgical outcome was specified as: Catastrophic Outcome (death or severe stroke), Psychiatric Complications, or Good Outcome (survival with no psychiatric complications). Inhospital outcome related significantly to analogy test scores, as did both surgical procedure and diagnosis of rheumatic heart disease. However, long-term outcome was unrelated to medical diagnosis and only weakly related to surgical procedure, but highly significantly related to preoperative analogy scores. The CLAT was a more consistent predictor of both short- and long-term outcome than any of the other ten variables considered (medical and surgical variables, inhospital outcome, demographic measures, other psychological tests).", "contents": "Analogy tests and psychopathology at follow-up after open heart surgery. A study of 100 coronary bypass and cardiac valvular surgery patients investigated whether preoperative brain damage, as measured by the Conceptual Level Analogy Test (CLAT), is a major risk factor for postoperative psychiatric symptoms and mortality. Three cognitive psychological tests, including the CLAT, and psychatric interviews were given preoperatively, postoperatively, and at 18-month follow-up. Surgical outcome was specified as: Catastrophic Outcome (death or severe stroke), Psychiatric Complications, or Good Outcome (survival with no psychiatric complications). Inhospital outcome related significantly to analogy test scores, as did both surgical procedure and diagnosis of rheumatic heart disease. However, long-term outcome was unrelated to medical diagnosis and only weakly related to surgical procedure, but highly significantly related to preoperative analogy scores. The CLAT was a more consistent predictor of both short- and long-term outcome than any of the other ten variables considered (medical and surgical variables, inhospital outcome, demographic measures, other psychological tests)."}
{"id": "PMID:1087165", "title": "Vestibular dysfunction in emotionally disturbed children.", "content": "Twenty-five emotionally disturbed children were tested on the Lafayette Clinic Cognitive Perceptual Motor (CPM) battery. The children were next examined for vestibular function by measuring ocular nystagmus for three different rotation rates. Under conditions of eye closure, children found to have two or more marked CPM deficits demonstrated a significantly reduced amplitude of postrotatory nystagmus as compared to CPM nondeficit children, and for the slowest rate of rotation (30 degrees/sec), a significantly decreased duration of nystagmus.", "contents": "Vestibular dysfunction in emotionally disturbed children. Twenty-five emotionally disturbed children were tested on the Lafayette Clinic Cognitive Perceptual Motor (CPM) battery. The children were next examined for vestibular function by measuring ocular nystagmus for three different rotation rates. Under conditions of eye closure, children found to have two or more marked CPM deficits demonstrated a significantly reduced amplitude of postrotatory nystagmus as compared to CPM nondeficit children, and for the slowest rate of rotation (30 degrees/sec), a significantly decreased duration of nystagmus."}
{"id": "PMID:1087166", "title": "The action of various sera on colony formation and DNA synthesis of murine thymocytes in culture.", "content": "Murine thymus cells were caltivated up to 10 days in a semi-solid medium. Both macroscopically visible colonies and DNA synthesis (identified by 3H-thymidine incorporation) were counted with and without added sera. According to their origins the sera exerted different modifications of the cultivated cells behaviour. Changes of colony number and of thymidine incorporation were quite independent on each other.", "contents": "The action of various sera on colony formation and DNA synthesis of murine thymocytes in culture. Murine thymus cells were caltivated up to 10 days in a semi-solid medium. Both macroscopically visible colonies and DNA synthesis (identified by 3H-thymidine incorporation) were counted with and without added sera. According to their origins the sera exerted different modifications of the cultivated cells behaviour. Changes of colony number and of thymidine incorporation were quite independent on each other."}
{"id": "PMID:1087167", "title": "An animal model for the study of human alpha-1-antitrypsin deficiency.", "content": "Turkeys with \"round heart disease\" often have a deficiency in alpha1 globulin. Within their liver cells, PAS +, diastase resistant granules are present, and are remarkably similar to those of persons with inherited AAT deficiency. The electrophoretic patterns made according Fagerhol's method showed differences between the serum of healthy birds and the turkeys suffering from \"round heart disease\".", "contents": "An animal model for the study of human alpha-1-antitrypsin deficiency. Turkeys with \"round heart disease\" often have a deficiency in alpha1 globulin. Within their liver cells, PAS +, diastase resistant granules are present, and are remarkably similar to those of persons with inherited AAT deficiency. The electrophoretic patterns made according Fagerhol's method showed differences between the serum of healthy birds and the turkeys suffering from \"round heart disease\"."}
{"id": "PMID:1087168", "title": "Acute lymphoblastic leukemia: a study of 25 cases by scanning electron microscopy.", "content": "Cells from 25 cases of acute lymphoblastic leukemia (ALL) were studied under the scanning electron microscope (SEM). In 24 of the cases, the vast majority of circulating leukaemic cells had few microvilli. Villous cells were rarely encountered and prominent ridge-like profiles and ruffled membranes were not seen. Only six cases were studied by immunological techniques and four of the cases were of the null type while in two the cells bore detectable T-markers. It seems that ALL is almost always associated with the presence of cells with few microvilli in the peripheral circulation, differing in this respect from most cases of CLL. Although circulating leukaemic lymphocytes with few microvilli are sometimes seen in CLL, the most frequent cell type encountered is a more villous lymphocyte. Differences between leukaemic cells from patients with ALL, CLL and non-lymphoblastic leukaemias are discussed. It appears that SEM may help to distinguish lymphoblastic and nonlymphoblastic leukaemic cells in many instances and can be used as a useful adjunct to other modes of microscopy in the diagnosis of acute leukaemia.", "contents": "Acute lymphoblastic leukemia: a study of 25 cases by scanning electron microscopy. Cells from 25 cases of acute lymphoblastic leukemia (ALL) were studied under the scanning electron microscope (SEM). In 24 of the cases, the vast majority of circulating leukaemic cells had few microvilli. Villous cells were rarely encountered and prominent ridge-like profiles and ruffled membranes were not seen. Only six cases were studied by immunological techniques and four of the cases were of the null type while in two the cells bore detectable T-markers. It seems that ALL is almost always associated with the presence of cells with few microvilli in the peripheral circulation, differing in this respect from most cases of CLL. Although circulating leukaemic lymphocytes with few microvilli are sometimes seen in CLL, the most frequent cell type encountered is a more villous lymphocyte. Differences between leukaemic cells from patients with ALL, CLL and non-lymphoblastic leukaemias are discussed. It appears that SEM may help to distinguish lymphoblastic and nonlymphoblastic leukaemic cells in many instances and can be used as a useful adjunct to other modes of microscopy in the diagnosis of acute leukaemia."}
{"id": "PMID:1087169", "title": "The small bowel enema:  description and experience of a technique.", "content": "A method of examining the small bowel, involving duodenal intubation is described. Good visualization of all parts of the small bowel is obtained using an air-barium double contrast technique. The technique is compared and contrasted with the more conventional methods of small bowel examination, and its advantages and disadvantages discussed. The authors relate their experience with the technique, indicating the abnormalities which can be shown, and list the type of clinical problems where the technique may be most usefully employed.", "contents": "The small bowel enema:  description and experience of a technique. A method of examining the small bowel, involving duodenal intubation is described. Good visualization of all parts of the small bowel is obtained using an air-barium double contrast technique. The technique is compared and contrasted with the more conventional methods of small bowel examination, and its advantages and disadvantages discussed. The authors relate their experience with the technique, indicating the abnormalities which can be shown, and list the type of clinical problems where the technique may be most usefully employed."}
{"id": "PMID:1087170", "title": "Lymphocytic infiltration of the brain in sleeping sickness.", "content": "Cerebrospinal fluid mononuclear cells from 40 patients with advanced Gambian sleeping sickness were examined for intracytoplasmic immunoglobulin and for B- and T-lymphocyte markers. About 5% of mononuclear cells were plasma cells. Most of the lymphocytes present were B cells. These findings suggest that the considerable lymphocytic infiltration of the nervous system seen in advanced sleeping sickness is not a cell-mediated immune reaction to trypanosomes. Immune complexes may play a part in producing the brain damage characteristic of this disease.", "contents": "Lymphocytic infiltration of the brain in sleeping sickness. Cerebrospinal fluid mononuclear cells from 40 patients with advanced Gambian sleeping sickness were examined for intracytoplasmic immunoglobulin and for B- and T-lymphocyte markers. About 5% of mononuclear cells were plasma cells. Most of the lymphocytes present were B cells. These findings suggest that the considerable lymphocytic infiltration of the nervous system seen in advanced sleeping sickness is not a cell-mediated immune reaction to trypanosomes. Immune complexes may play a part in producing the brain damage characteristic of this disease."}
{"id": "PMID:1087178", "title": "Separation of mononuclear leukocytes and polymorphonuclear leukocytes from equine blood.", "content": "The present study describes a two step technique for the separation of mononuclear leukocytes or mononuclear and polymorphonuclear leukocytes from whole equine blood. First, the leukocyte rich plasma was obtained by sedimentation of erythrocytes in the undiluted blood. Subsequently, separation of the different populations of white blood cells was performed by centrifugation with different gradients overlaid with the leukocyte rich plasma. The optimal separation of the mononuclear cells was obtained by the centrifugation of the leukocyte rich plasma overlaying the gradient containing 24 parts of 9.5% ficoll and ten parts of 34% isopaque. The mononuclear leukocytes (95% lymphocytes and 5% monocytes) formed a monolayer band at the plasma-ficoll-isopaque interface and other blood cells migrated to the bottom of the tube. For the separation of mononuclear and granular leukocytes from the blood, the gradient containing 24 parts of 10% ficoll and ten parts of 34% isopaque was used. The separated monuclear leukocytes responded to stimulation with phytohemagglutin and viability of both mononuclear and polymorphonuclear leukocytes was not affected by ficoll-isopaque separation.", "contents": "Separation of mononuclear leukocytes and polymorphonuclear leukocytes from equine blood. The present study describes a two step technique for the separation of mononuclear leukocytes or mononuclear and polymorphonuclear leukocytes from whole equine blood. First, the leukocyte rich plasma was obtained by sedimentation of erythrocytes in the undiluted blood. Subsequently, separation of the different populations of white blood cells was performed by centrifugation with different gradients overlaid with the leukocyte rich plasma. The optimal separation of the mononuclear cells was obtained by the centrifugation of the leukocyte rich plasma overlaying the gradient containing 24 parts of 9.5% ficoll and ten parts of 34% isopaque. The mononuclear leukocytes (95% lymphocytes and 5% monocytes) formed a monolayer band at the plasma-ficoll-isopaque interface and other blood cells migrated to the bottom of the tube. For the separation of mononuclear and granular leukocytes from the blood, the gradient containing 24 parts of 10% ficoll and ten parts of 34% isopaque was used. The separated monuclear leukocytes responded to stimulation with phytohemagglutin and viability of both mononuclear and polymorphonuclear leukocytes was not affected by ficoll-isopaque separation."}
{"id": "PMID:1087179", "title": "Clinical description and roentgenologic evaluation of patients with Friedreich's ataxia.", "content": "The 50 patients in this survey were classified by a panel of neurologists into 4 clinical sub-groups: Group Ia (\"typical\" Friedreich's ataxia, complete picture), Group Ib (\"typical\" Friedreich's ataxia, incomplete picture), Group IIa (\"atypical\" Frriedreich's ataxia, possible recessive Roussy-Levy syndrome), Group IIb (heterogeneous ataxias). The clinical symptoms and signs were analyzed for each of these groups. A constellation of signs constantly present in Friedreich's ataxia and obligatory for diagnosis was described. Other important symptoms, such as the Babinski sign, kyphoscoliosis and pes cavus were found to be progressive, but not essential for the diagnosis  at any given time. Finally, a host of other symptoms can only be called accessory. The progression of scoliosis was found to be an important tool in the differential diagnosis of ataxias. Our study also indicates, in contrast to the opinion of some authors, that absent deep tendon reflexes in the lower limbs and early dysarthria are essential in \"typical\" Friedreich's ataxia.", "contents": "Clinical description and roentgenologic evaluation of patients with Friedreich's ataxia. The 50 patients in this survey were classified by a panel of neurologists into 4 clinical sub-groups: Group Ia (\"typical\" Friedreich's ataxia, complete picture), Group Ib (\"typical\" Friedreich's ataxia, incomplete picture), Group IIa (\"atypical\" Frriedreich's ataxia, possible recessive Roussy-Levy syndrome), Group IIb (heterogeneous ataxias). The clinical symptoms and signs were analyzed for each of these groups. A constellation of signs constantly present in Friedreich's ataxia and obligatory for diagnosis was described. Other important symptoms, such as the Babinski sign, kyphoscoliosis and pes cavus were found to be progressive, but not essential for the diagnosis  at any given time. Finally, a host of other symptoms can only be called accessory. The progression of scoliosis was found to be an important tool in the differential diagnosis of ataxias. Our study also indicates, in contrast to the opinion of some authors, that absent deep tendon reflexes in the lower limbs and early dysarthria are essential in \"typical\" Friedreich's ataxia."}
{"id": "PMID:1087180", "title": "The reversal of methotrexate cytotoxicity to mouse bone marrow cells by leucovorin and nucleosides.", "content": "The cytotoxic effect of methotrexate (MTX) for mouse bone marrow cells has been studied by in vitro of the granulocyte precursor cell (CFU-C) in a medium containing dialyzed fetal calf serum and dialyzed L-cell supernatant. The formation of 50-cell colonies was inhibited to 50% of control by 10(-8) M MTX. Further increases in MTX concentration rapidly abolished colony formation by CFU-C. The potential of leucovorin and nucleosides to rescue the CFU-C from MTX toxicity was studied. Toxicity of 10(-7) M MTX was completely reversed by equimolar concentrations of leucovorin, but with higher MTX concentrations, relatively more leucovorin was required. While 10(-5) M MTX was rescued by 10(-3) M leucovorin, rescue of the toxic effect of 10(-4) M MTX by 10(-3) M leucovorin was not observed. In contrast to the rescue by Leucovorin, toxicity of all MTX concentrations up to 10(-4) M was completely prevented by 10(-5) M thymidine with 10(-5) M adenosine, inosine, or hypoxanthine. Single nucleosides or thymidine with guanosine were ineffective, as were lower concentrations (less than or equal to 10(-6)M) of the effective combinations. Thus, while leucovorin reversed the MTX toxicity to CFU-C competitively, rescue by nucleosides was noncompetitive. The significance and possible usefulness of these findings for chemotherapeutic protocols are discussed.", "contents": "The reversal of methotrexate cytotoxicity to mouse bone marrow cells by leucovorin and nucleosides. The cytotoxic effect of methotrexate (MTX) for mouse bone marrow cells has been studied by in vitro of the granulocyte precursor cell (CFU-C) in a medium containing dialyzed fetal calf serum and dialyzed L-cell supernatant. The formation of 50-cell colonies was inhibited to 50% of control by 10(-8) M MTX. Further increases in MTX concentration rapidly abolished colony formation by CFU-C. The potential of leucovorin and nucleosides to rescue the CFU-C from MTX toxicity was studied. Toxicity of 10(-7) M MTX was completely reversed by equimolar concentrations of leucovorin, but with higher MTX concentrations, relatively more leucovorin was required. While 10(-5) M MTX was rescued by 10(-3) M leucovorin, rescue of the toxic effect of 10(-4) M MTX by 10(-3) M leucovorin was not observed. In contrast to the rescue by Leucovorin, toxicity of all MTX concentrations up to 10(-4) M was completely prevented by 10(-5) M thymidine with 10(-5) M adenosine, inosine, or hypoxanthine. Single nucleosides or thymidine with guanosine were ineffective, as were lower concentrations (less than or equal to 10(-6)M) of the effective combinations. Thus, while leucovorin reversed the MTX toxicity to CFU-C competitively, rescue by nucleosides was noncompetitive. The significance and possible usefulness of these findings for chemotherapeutic protocols are discussed."}
{"id": "PMID:1087181", "title": "The stimulation by methotrexate of human chorionic gonadotropin and placental alkaline phosphatase in cultured choriocarcinoma cells.", "content": "Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a tenfold increase in synthesis of human chorionic gonadotropin and a threefold increase in activity of placental alkaline phosphatase. No concomitant increase in lactic dehydrogenase activity occurs under these conditions. This effect of methotrexate can be blocked by simultaneous addition of thymidine or folinic acid, neither of which alone increases human chorionic gonadotropin synthesis or placental alkaline phosphatase activity in BeWo cells.", "contents": "The stimulation by methotrexate of human chorionic gonadotropin and placental alkaline phosphatase in cultured choriocarcinoma cells. Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a tenfold increase in synthesis of human chorionic gonadotropin and a threefold increase in activity of placental alkaline phosphatase. No concomitant increase in lactic dehydrogenase activity occurs under these conditions. This effect of methotrexate can be blocked by simultaneous addition of thymidine or folinic acid, neither of which alone increases human chorionic gonadotropin synthesis or placental alkaline phosphatase activity in BeWo cells."}
{"id": "PMID:1087182", "title": "Biochemical and pharmacokinetic effects of leucovorin after high-dose methotrexate in a murine leukemia model.", "content": "The administration of calcium leucovorin, either concurrently or after high dosages of methotrexate in L1210 leukemic mice, has both pharmacokinetic and biochemical effects in tumor cells and drug-limiting proliferative  normal tissue in small intestine. A reduction in the maximal level of accumulation and retention of exchangeable drug (unbound to dihydrofolate reductase) in tissue could be demonstrated when calcium leucovorin was given simultaneously with methotrexate at equal or greater dosages than the latter. The dose dependence for calcium leucovorin-introduced drug loss is similar in both tissues and showed the expected variation when the time interval between methotrexate and calcium leucovorin doses was increased. With 400 mg methotrexate per kg, greater than 96 mg calcium leucovorin per kg were required maximally to affect overall drug retention in tissue 2 hr after drug, whereas only 24 mg calcium leucovorin per kg were required 16 hr after drug. Calcium leucovorin, given after methotrexate, induced synchronous recovery of DNA synthesis (measured by labeled deoxyruridine incorporation) in both small intestine and L1210 cells. An initial cycle of synthesis was induced in the presence of exchangeable levels of drug. Two hr after methotrexate, 12 mg/kg, calcium leucovorin induced an immediate but only partial (20 to 25% of control rate) recovery of synthesis with dose dependence from 3 to 12 mg calcium leucovorin per kg. Less synthesis was induced after 96 mg/kg and almost none after methotrexate, 400 mg/kg. With calcium leucovorin, 24 mg/kg, given 2 hr after methotrexate, 12 or 96 mg/kg, a major cycle of synthesis occurred when total drug levels approached the equivalence of the dihydrofolate reductase content. The magnitude of this cycle of synthesis in both L1210 cells and small intestine was the same as that seen in animals recovering from methotrexate alone. However, this is based on the assumption that an approximately equivalent relationship between DNA synthesis and labeled doexyuridine incorporation in each tissue during the period of maximal incorporation within the cycle. The major effect of calcium leucovorin, then, was to induce an earlier resumption of DNA synthesis as a consequence of the pharmacokinetic effect in each tissue. With calcium leucovorin, 24 or 400 mg/kg, given 16 hr after methotrexate, an identical effect on drug retention was observed in both L1210 cells and small intestine. Although there was a difference in the time course for recovery in small intestine at each dosage of calcium leucovorin, the recovery of DNA synthesis as drug levels approached the dihydrofolate reductase content was similar in magnitude. In L1210 cells, however, substantial recovery of synthesis to a comparable level and with a similar time-course occurred only after leucovorin, 400 mg/kg. Little or no recovery of DNA synthesis was observed after calcium leucovorin, 24 mg/kg, during the same time period. This dosage schedule (methotrexate, 400 mg/kg, s.c...", "contents": "Biochemical and pharmacokinetic effects of leucovorin after high-dose methotrexate in a murine leukemia model. The administration of calcium leucovorin, either concurrently or after high dosages of methotrexate in L1210 leukemic mice, has both pharmacokinetic and biochemical effects in tumor cells and drug-limiting proliferative  normal tissue in small intestine. A reduction in the maximal level of accumulation and retention of exchangeable drug (unbound to dihydrofolate reductase) in tissue could be demonstrated when calcium leucovorin was given simultaneously with methotrexate at equal or greater dosages than the latter. The dose dependence for calcium leucovorin-introduced drug loss is similar in both tissues and showed the expected variation when the time interval between methotrexate and calcium leucovorin doses was increased. With 400 mg methotrexate per kg, greater than 96 mg calcium leucovorin per kg were required maximally to affect overall drug retention in tissue 2 hr after drug, whereas only 24 mg calcium leucovorin per kg were required 16 hr after drug. Calcium leucovorin, given after methotrexate, induced synchronous recovery of DNA synthesis (measured by labeled deoxyruridine incorporation) in both small intestine and L1210 cells. An initial cycle of synthesis was induced in the presence of exchangeable levels of drug. Two hr after methotrexate, 12 mg/kg, calcium leucovorin induced an immediate but only partial (20 to 25% of control rate) recovery of synthesis with dose dependence from 3 to 12 mg calcium leucovorin per kg. Less synthesis was induced after 96 mg/kg and almost none after methotrexate, 400 mg/kg. With calcium leucovorin, 24 mg/kg, given 2 hr after methotrexate, 12 or 96 mg/kg, a major cycle of synthesis occurred when total drug levels approached the equivalence of the dihydrofolate reductase content. The magnitude of this cycle of synthesis in both L1210 cells and small intestine was the same as that seen in animals recovering from methotrexate alone. However, this is based on the assumption that an approximately equivalent relationship between DNA synthesis and labeled doexyuridine incorporation in each tissue during the period of maximal incorporation within the cycle. The major effect of calcium leucovorin, then, was to induce an earlier resumption of DNA synthesis as a consequence of the pharmacokinetic effect in each tissue. With calcium leucovorin, 24 or 400 mg/kg, given 16 hr after methotrexate, an identical effect on drug retention was observed in both L1210 cells and small intestine. Although there was a difference in the time course for recovery in small intestine at each dosage of calcium leucovorin, the recovery of DNA synthesis as drug levels approached the dihydrofolate reductase content was similar in magnitude. In L1210 cells, however, substantial recovery of synthesis to a comparable level and with a similar time-course occurred only after leucovorin, 400 mg/kg. Little or no recovery of DNA synthesis was observed after calcium leucovorin, 24 mg/kg, during the same time period. This dosage schedule (methotrexate, 400 mg/kg, s.c..."}
{"id": "PMID:1087183", "title": "Identification of the epidermal \"Stiftchenzellen\" of frog tadpoles by electron microscopy.", "content": "Differentiated surface epidermal cells observed in the skin of tadpoles of Rana temporaria by electron microscopy have been identified with the Stiftchenzellen originally described by K\u00f6lliker in 1885. The cells have apical microvilli or a single apical projection and appear to have synaptic associations with nerve fibres in the epidermis. The distribution, dimensions and structure of the cells are in agreement with descriptions from le cells are sensory in nature. In addition, there are fine structural resemblances to the gustatory cells of fish and of amphibians which suggest that the Stiftchenzellen are chemoreceptors.", "contents": "Identification of the epidermal \"Stiftchenzellen\" of frog tadpoles by electron microscopy. Differentiated surface epidermal cells observed in the skin of tadpoles of Rana temporaria by electron microscopy have been identified with the Stiftchenzellen originally described by K\u00f6lliker in 1885. The cells have apical microvilli or a single apical projection and appear to have synaptic associations with nerve fibres in the epidermis. The distribution, dimensions and structure of the cells are in agreement with descriptions from le cells are sensory in nature. In addition, there are fine structural resemblances to the gustatory cells of fish and of amphibians which suggest that the Stiftchenzellen are chemoreceptors."}
{"id": "PMID:1087187", "title": "Atherosclerosis of the aorta and coronary arteries in five towns. Material and methods.", "content": "This chapter outlines the way in which the problems of obtaining and assessing population-related material and analysing the data were tackled. Some of the limits of the approach used, namely, the examination of nearly all deaths from several demographically defined communities, are discussed.", "contents": "Atherosclerosis of the aorta and coronary arteries in five towns. Material and methods. This chapter outlines the way in which the problems of obtaining and assessing population-related material and analysing the data were tackled. Some of the limits of the approach used, namely, the examination of nearly all deaths from several demographically defined communities, are discussed."}
{"id": "PMID:1087188", "title": "Atherosclerosis of the aorta in five towns.", "content": "Fatty streak was always present in both the thoracic aorta and the abdominal aorta in the youngest subjects studied (aged 10-14 years). Fibrous plaque was present in a small proportion of these young subjects, but a rapid increase in prevalence occurred as early as the fourth decade. Complicated and calcified lesions appeared as early as the age of 20-25 years but a rapid increase in prevalence was seen after age 40 for complicated lesions and after age 50 for calcified lesions. There were differences in the prevalence of severe lesions among the five towns. There was little increase in the extent of atherosclerosis in the thoracic aorta before the age of 40 and in the abdominal aorta before the age of 20. The increase was more rapid after those ages. When atherosclerosis had affected about 50% of the intimal surface of the thoracic aorta and 70% of the intimal surface of the abdominal aorta, the increase slowed down considerably. In contrast to other types of lesion, the extent of fatty streak increased only up to 30 years of age, when it occupied 25-30% of the intimal surface. Then it declined and in the older age groups did not exceed 4-5% in men or women. The extent of fibrous plaque and complicated lesions was at all ages greater in men than in women, while the extent of fatty streak and calcified lesions in older age groups was greater in women. There were marked differences in the extent of atherosclerotic lesions in the five towns.", "contents": "Atherosclerosis of the aorta in five towns. Fatty streak was always present in both the thoracic aorta and the abdominal aorta in the youngest subjects studied (aged 10-14 years). Fibrous plaque was present in a small proportion of these young subjects, but a rapid increase in prevalence occurred as early as the fourth decade. Complicated and calcified lesions appeared as early as the age of 20-25 years but a rapid increase in prevalence was seen after age 40 for complicated lesions and after age 50 for calcified lesions. There were differences in the prevalence of severe lesions among the five towns. There was little increase in the extent of atherosclerosis in the thoracic aorta before the age of 40 and in the abdominal aorta before the age of 20. The increase was more rapid after those ages. When atherosclerosis had affected about 50% of the intimal surface of the thoracic aorta and 70% of the intimal surface of the abdominal aorta, the increase slowed down considerably. In contrast to other types of lesion, the extent of fatty streak increased only up to 30 years of age, when it occupied 25-30% of the intimal surface. Then it declined and in the older age groups did not exceed 4-5% in men or women. The extent of fibrous plaque and complicated lesions was at all ages greater in men than in women, while the extent of fatty streak and calcified lesions in older age groups was greater in women. There were marked differences in the extent of atherosclerotic lesions in the five towns."}
{"id": "PMID:1087189", "title": "Atherosclerosis of the coronary arteries in five towns.", "content": "Two atherosclerotic lesions (fatty streak and fibrous plaque) were seen in coronary arteries as early as in the 10-14 age group. Then their frequency increased with age, more rapidly in the left anterior descending coronary artery than in the left circumflex artery. After the age of 55 years fatty streak was still the sole type of lesion in 0.3% of men and an even higher proportion of women. In contrast to the findings in the aorta, the mean extent of fatty streak in the average coronary artery did not exceed 3-4% in all age groups. Fibrous plaque was found in over 90% of men over 40 and women over 50 years of age. The extent of fibrous plaque was greatest by about the age of 65 years in both sexes and showed little change thereafter. The first complicated and calcified lesions appeared in a small proportion of subjects after the age of 20. The frequency of complicated lesions did not exceed 50%, being much lower than that of calcified lesions. Complicated lesions occupied a very small area (mean extent 0.6% in men and 0.3% in women); the extent of calcified lesions in older age groups reached 9-10% of the intimal surface in both sexes. Coronary stenosis appeared after age 20 years in men and 10 years later in women, and its frequency increased with age. There were considerable inter-town differences in the prevalence and extent of atherosclerotic lesions.", "contents": "Atherosclerosis of the coronary arteries in five towns. Two atherosclerotic lesions (fatty streak and fibrous plaque) were seen in coronary arteries as early as in the 10-14 age group. Then their frequency increased with age, more rapidly in the left anterior descending coronary artery than in the left circumflex artery. After the age of 55 years fatty streak was still the sole type of lesion in 0.3% of men and an even higher proportion of women. In contrast to the findings in the aorta, the mean extent of fatty streak in the average coronary artery did not exceed 3-4% in all age groups. Fibrous plaque was found in over 90% of men over 40 and women over 50 years of age. The extent of fibrous plaque was greatest by about the age of 65 years in both sexes and showed little change thereafter. The first complicated and calcified lesions appeared in a small proportion of subjects after the age of 20. The frequency of complicated lesions did not exceed 50%, being much lower than that of calcified lesions. Complicated lesions occupied a very small area (mean extent 0.6% in men and 0.3% in women); the extent of calcified lesions in older age groups reached 9-10% of the intimal surface in both sexes. Coronary stenosis appeared after age 20 years in men and 10 years later in women, and its frequency increased with age. There were considerable inter-town differences in the prevalence and extent of atherosclerotic lesions."}
{"id": "PMID:1087190", "title": "High and low atherosclerosis groups.", "content": "Aortic and coronary atherosclerosis were studied in two groups, the \"high atherosclerosis group\" and the \"low atherosclerosis group\". The latter may be considered as showing basic levels of atherosclerosis in the different communities. The development of lesions in the two groups occurred in parallel, but earlier in the high group. Sex differences in the two groups were similar to those in the whole material. Generally speaking, inter-town comparisons were similar in each group and similar to those for deaths from all causes, a notable exception being the prevalence of coronary stenosis. The findings indicate that the groups can be used as reference groups, but only for material drawn from the same source.", "contents": "High and low atherosclerosis groups. Aortic and coronary atherosclerosis were studied in two groups, the \"high atherosclerosis group\" and the \"low atherosclerosis group\". The latter may be considered as showing basic levels of atherosclerosis in the different communities. The development of lesions in the two groups occurred in parallel, but earlier in the high group. Sex differences in the two groups were similar to those in the whole material. Generally speaking, inter-town comparisons were similar in each group and similar to those for deaths from all causes, a notable exception being the prevalence of coronary stenosis. The findings indicate that the groups can be used as reference groups, but only for material drawn from the same source."}
{"id": "PMID:1087191", "title": "Atherosclerosis and sudden death.", "content": "The prevalence of complicated and calcified lesions and coronary stenosis, the mean heart weight, and the extent of atherosclerosis in the aorta and coronary arteries were greater in the \"sudden heart death\" group than in the high atherosclerosis group. In the \"other sudden death\" group, which included sudden deaths without myocardial infarction or coronary occlusion, all the above variables, except heart weight, were found to be lower than in the low atherosclerosis group and were close to those in the standardized average atherosclerosis group. The mean heart weight in the \"other sudden death\" group was lower than in the \"sudden heart death\" group but significantly higher than in the three reference atherosclerosis groups.", "contents": "Atherosclerosis and sudden death. The prevalence of complicated and calcified lesions and coronary stenosis, the mean heart weight, and the extent of atherosclerosis in the aorta and coronary arteries were greater in the \"sudden heart death\" group than in the high atherosclerosis group. In the \"other sudden death\" group, which included sudden deaths without myocardial infarction or coronary occlusion, all the above variables, except heart weight, were found to be lower than in the low atherosclerosis group and were close to those in the standardized average atherosclerosis group. The mean heart weight in the \"other sudden death\" group was lower than in the \"sudden heart death\" group but significantly higher than in the three reference atherosclerosis groups."}
{"id": "PMID:1087192", "title": "Atherosclerosis in relation to cholelithiasis and cholesterolosis.", "content": "The frequency of cholelithiasis and cholesterolosis was remarkably similar in Prague and Malm\u00f6 subjects. When the possible effects of associated hypertension or diabetes mellitus were taken into account, subjects with cholelithiasis showed the same extent of raised and calcified lesions of the coronary arteries, the same prevalence of large myocardial scars, and the same distribution of heart weight as subjects without cholelithiasis. In general, they showed rather fewer raised and calcified lesions of the aorta, less coronary stenosis, and fewer fresh myocardial infarctions than subjects without cholelithiasis. Subjects with cholesterolosis were similar to those without this condition in respect of raised lesions of the aorta and coronary arteries. Men with cholesterolosis had slightly more aortic calcification and slightly less coronary calcification. Women with cholesterolosis had slightly less coronary stenosis than those without cholesterolosis. There was a slight tendency for those with cholesterolosis to show an increased frequency of fresh myocardial infarction and large myocardial scars and to have a higher heart weight.", "contents": "Atherosclerosis in relation to cholelithiasis and cholesterolosis. The frequency of cholelithiasis and cholesterolosis was remarkably similar in Prague and Malm\u00f6 subjects. When the possible effects of associated hypertension or diabetes mellitus were taken into account, subjects with cholelithiasis showed the same extent of raised and calcified lesions of the coronary arteries, the same prevalence of large myocardial scars, and the same distribution of heart weight as subjects without cholelithiasis. In general, they showed rather fewer raised and calcified lesions of the aorta, less coronary stenosis, and fewer fresh myocardial infarctions than subjects without cholelithiasis. Subjects with cholesterolosis were similar to those without this condition in respect of raised lesions of the aorta and coronary arteries. Men with cholesterolosis had slightly more aortic calcification and slightly less coronary calcification. Women with cholesterolosis had slightly less coronary stenosis than those without cholesterolosis. There was a slight tendency for those with cholesterolosis to show an increased frequency of fresh myocardial infarction and large myocardial scars and to have a higher heart weight."}
{"id": "PMID:1087193", "title": "Atherosclerosis and hypertension.", "content": "Autopsy studies of atherosclerosis of the aorta and the coronary arteries were carried out in 3134 subjects with essential hypertension. A comparison was made with low, average, and high atherosclerosis groups. Essential hypertension was found to accelerate the development of all types of aortic lesion, except fatty streak, as compared with the standardized average atherosclerosis group, and to accelerate the development of fibrous plaque but not complicated and calcified lesions as compared with the high atherosclerosis group. The extent of fibrous plaque in the coronary arteries was greater in the essential hypertension group than in the low and standardized average atherosclerosis groups but did not differ from that in the high atherosclerosis group. The extent of complicated and calcified lesions and the prevalence of coronary stenosis were higher in the high atherosclerosis group than in cases of hypertension. Geographical differences in atherosclerosis among hypertensives in different towns reflected the findings for the whole material. Symptomatic hypertension was found to accelerate aortic atherosclerosis at least to the same extent as essential hypertension. It was conductive to coronary atherosclerosis but not to the same extent as essential hypertension. Coronary stenosis and various manifestations of coronary heart disease were rare in symptomatic hypertension.", "contents": "Atherosclerosis and hypertension. Autopsy studies of atherosclerosis of the aorta and the coronary arteries were carried out in 3134 subjects with essential hypertension. A comparison was made with low, average, and high atherosclerosis groups. Essential hypertension was found to accelerate the development of all types of aortic lesion, except fatty streak, as compared with the standardized average atherosclerosis group, and to accelerate the development of fibrous plaque but not complicated and calcified lesions as compared with the high atherosclerosis group. The extent of fibrous plaque in the coronary arteries was greater in the essential hypertension group than in the low and standardized average atherosclerosis groups but did not differ from that in the high atherosclerosis group. The extent of complicated and calcified lesions and the prevalence of coronary stenosis were higher in the high atherosclerosis group than in cases of hypertension. Geographical differences in atherosclerosis among hypertensives in different towns reflected the findings for the whole material. Symptomatic hypertension was found to accelerate aortic atherosclerosis at least to the same extent as essential hypertension. It was conductive to coronary atherosclerosis but not to the same extent as essential hypertension. Coronary stenosis and various manifestations of coronary heart disease were rare in symptomatic hypertension."}
{"id": "PMID:1087194", "title": "Atherosclerosis and diabetes mellitus.", "content": "Diabetes mellitus occurred most frequently in Prague (8.4%) and Malm\u00f6 (8.6%), and less often in Tallin (3.4%), Yalta (3.3%), and Ryazan (2.1%), these differences not being connected with differences in age distribution. The extent of raised and calcified lesions in the coronary arteries and calcified lesions in the aorta in the pure diabetes group was similar to that in the high atherosclerosis group and significantly higher than in the standardized average atherosclerosis group. Raised and calcified lesions in the aorta and raised lesions in the coronary arteries were more extensive in the diabetes group than in the high atherosclerosis group. In all diabetes groups, stenosis of the coronary arteries and myocardial lesions occurred much more frequently than in the average atherosclerosis group but were less common than in the high atherosclerosis group. Coronary thrombosis also occurred more frequently in the high atherosclerosis group. Diabetes per se and hypertension were found to have approximately the same effect on the development of atherosclerosis, but hypertension had a greater effect than diabetes on the development of aortic atherosclerosis. There were no significant differences in the frequency of coronary stenosis and myocardial lesions in diabetics and hypertensives. Diabetics of more than 10 years' standing generally showed more coronary but not more aortic atherosclerosis than those who had had diabetes for less than 10 years. In insulin-treated diabetics coronary atherosclerosis tended to be more extensive than in those treated by diet or by tablets, but there was no difference in the frequency of coronary stenosis and thrombosis and myocardial and cerebrovascular lesions.", "contents": "Atherosclerosis and diabetes mellitus. Diabetes mellitus occurred most frequently in Prague (8.4%) and Malm\u00f6 (8.6%), and less often in Tallin (3.4%), Yalta (3.3%), and Ryazan (2.1%), these differences not being connected with differences in age distribution. The extent of raised and calcified lesions in the coronary arteries and calcified lesions in the aorta in the pure diabetes group was similar to that in the high atherosclerosis group and significantly higher than in the standardized average atherosclerosis group. Raised and calcified lesions in the aorta and raised lesions in the coronary arteries were more extensive in the diabetes group than in the high atherosclerosis group. In all diabetes groups, stenosis of the coronary arteries and myocardial lesions occurred much more frequently than in the average atherosclerosis group but were less common than in the high atherosclerosis group. Coronary thrombosis also occurred more frequently in the high atherosclerosis group. Diabetes per se and hypertension were found to have approximately the same effect on the development of atherosclerosis, but hypertension had a greater effect than diabetes on the development of aortic atherosclerosis. There were no significant differences in the frequency of coronary stenosis and myocardial lesions in diabetics and hypertensives. Diabetics of more than 10 years' standing generally showed more coronary but not more aortic atherosclerosis than those who had had diabetes for less than 10 years. In insulin-treated diabetics coronary atherosclerosis tended to be more extensive than in those treated by diet or by tablets, but there was no difference in the frequency of coronary stenosis and thrombosis and myocardial and cerebrovascular lesions."}
{"id": "PMID:1087195", "title": "Atherosclerosis and malignant tumours.", "content": "Aortic and coronary atherosclerosis were studied in subjects with a malignant disease and compared with those in the three atherosclerosis reference groups. In general, subjects with a malignant disease had little atherosclerosis, except for men with lung or prostatic cancer; in particular, men with lung cancer tended to have more extensive aortic atherosclerosis. Atherosclerosis was less extensive in subjects with tumours specific to females. The ratios between the different types of lesions was preserved and the change was therefore quantitative rather than qualitative. No proof of a real negative influence on atherosclerosis by malignant diseases was found but wasting could be one factor influencing the development of atherosclerosis in tumour subjects. Such subjects, except those with lung and prostatic cancer, could have been included in the low atherosclerosis group which was an index of the mean basic level of atherosclerosis in the populations studied.", "contents": "Atherosclerosis and malignant tumours. Aortic and coronary atherosclerosis were studied in subjects with a malignant disease and compared with those in the three atherosclerosis reference groups. In general, subjects with a malignant disease had little atherosclerosis, except for men with lung or prostatic cancer; in particular, men with lung cancer tended to have more extensive aortic atherosclerosis. Atherosclerosis was less extensive in subjects with tumours specific to females. The ratios between the different types of lesions was preserved and the change was therefore quantitative rather than qualitative. No proof of a real negative influence on atherosclerosis by malignant diseases was found but wasting could be one factor influencing the development of atherosclerosis in tumour subjects. Such subjects, except those with lung and prostatic cancer, could have been included in the low atherosclerosis group which was an index of the mean basic level of atherosclerosis in the populations studied."}
{"id": "PMID:1087196", "title": "Atherosclerosis and rheumatic heart disease.", "content": "The extent of aortic atherosclerosis in subjects with rheumatic heart diseases was similar to that in the standardized average atherosclerosis group of subjects. The extent of coronary atherosclerosis, particularly in men, was similar to that in the low atherosclerosis group. In the aorta, this finding was accounted for by the greater extent of complicated and raised lesions; in the coronary arteries, it was accounted for by the lesser extent of fibrous plaque. Coronary stenosis, fresh myocardial infarction, and large myocardial scar occurred much less frequently in rheumatic subjects than in the high atherosclerosis group. There was no difference in the frequency of stenosis between the rheumatic and low atherosclerosis groups.", "contents": "Atherosclerosis and rheumatic heart disease. The extent of aortic atherosclerosis in subjects with rheumatic heart diseases was similar to that in the standardized average atherosclerosis group of subjects. The extent of coronary atherosclerosis, particularly in men, was similar to that in the low atherosclerosis group. In the aorta, this finding was accounted for by the greater extent of complicated and raised lesions; in the coronary arteries, it was accounted for by the lesser extent of fibrous plaque. Coronary stenosis, fresh myocardial infarction, and large myocardial scar occurred much less frequently in rheumatic subjects than in the high atherosclerosis group. There was no difference in the frequency of stenosis between the rheumatic and low atherosclerosis groups."}
{"id": "PMID:1087197", "title": "Atherosclerosis and cirrhosis of the liver.", "content": "Atherosclerosis of the aorta and coronary arteries and myocardial infarction were studied post mortem in 390 males and 190 female cirrhotic subjects in the 5 towns. Comparison with the reference groups revealed that calcification of the aorta and coronary arteries was more frequent (in the case of males) and more extensive (in the case of males and females) in cirrhotics than in noncirrhotics. Raised and complicated lesions were not increased. Coronary stenosis (in females), fresh myocardial infarction (in both sexes), and large myocardial scar (in both sexes) were less frequent in cirrhotics. \"Obesity\" was similar in cirrhotics and non-cirrhotics.", "contents": "Atherosclerosis and cirrhosis of the liver. Atherosclerosis of the aorta and coronary arteries and myocardial infarction were studied post mortem in 390 males and 190 female cirrhotic subjects in the 5 towns. Comparison with the reference groups revealed that calcification of the aorta and coronary arteries was more frequent (in the case of males) and more extensive (in the case of males and females) in cirrhotics than in noncirrhotics. Raised and complicated lesions were not increased. Coronary stenosis (in females), fresh myocardial infarction (in both sexes), and large myocardial scar (in both sexes) were less frequent in cirrhotics. \"Obesity\" was similar in cirrhotics and non-cirrhotics."}
{"id": "PMID:1087198", "title": "Atherosclerosis and peptic ulcer.", "content": "Aortic and coronary atherosclerosis and the frequency of coronary stenosis and myocardial lesions were studied in subjects with peptic ulcer, in two subgroups with acute and chronic peptic ulcer, and in subjects who had undergone a stomach operation. In all these respects the groups were similar to each other. Men with peptic ulcer had the same amount of aortic atherosclerosis as the standardized average coronary atherosclerosis group but less coronary atherosclerosis, while women with peptic ulcer had less aortic and much less coronary atherosclerosis. The prevalence of coronary stenosis, fresh myocardial infarction, and myocardial scar was very low in those with peptic ulcer, especially in women.", "contents": "Atherosclerosis and peptic ulcer. Aortic and coronary atherosclerosis and the frequency of coronary stenosis and myocardial lesions were studied in subjects with peptic ulcer, in two subgroups with acute and chronic peptic ulcer, and in subjects who had undergone a stomach operation. In all these respects the groups were similar to each other. Men with peptic ulcer had the same amount of aortic atherosclerosis as the standardized average coronary atherosclerosis group but less coronary atherosclerosis, while women with peptic ulcer had less aortic and much less coronary atherosclerosis. The prevalence of coronary stenosis, fresh myocardial infarction, and myocardial scar was very low in those with peptic ulcer, especially in women."}
{"id": "PMID:1087199", "title": "Atherosclerosis in relation to tuberculosis and chronic lung disease.", "content": "The relation between atherosclerosis and tuberculosis and other chronic lung diseases was studied in extensive autopsy material. Aortic atherosclerosis in subjects with such conditions was generally similar in extent to that in the standardized atherosclerosis group and more extensive than that in the low atherosclerosis group, while coronary atherosclerosis was less extensive than in the standardized average group and, especially in women, approximated to that in the low atherosclerosis group. The prevalence of coronary stenosis and myocardial lesions was very low in subjects with tuberculosis and other chronic lung diseases. The presence of such diseases seemed to increase the development of atherosclerosis in the thoracic part of the aorta in relation to the abdominal part.", "contents": "Atherosclerosis in relation to tuberculosis and chronic lung disease. The relation between atherosclerosis and tuberculosis and other chronic lung diseases was studied in extensive autopsy material. Aortic atherosclerosis in subjects with such conditions was generally similar in extent to that in the standardized atherosclerosis group and more extensive than that in the low atherosclerosis group, while coronary atherosclerosis was less extensive than in the standardized average group and, especially in women, approximated to that in the low atherosclerosis group. The prevalence of coronary stenosis and myocardial lesions was very low in subjects with tuberculosis and other chronic lung diseases. The presence of such diseases seemed to increase the development of atherosclerosis in the thoracic part of the aorta in relation to the abdominal part."}
{"id": "PMID:1087200", "title": "Atherosclerosis of the aorta and coronary arteries in coronary heart disease.", "content": "Aortic and coronary atherosclerosis and the prevalence of coronary stenosis and thrombosis were studied in subjects who had died of fresh or recurrent myocardial infarction or had suffered from myocardial infarction in the past. In general, severe atherosclerosis of the coronary arteries with stenosis and calcification was almost a prerequisite for the development of coronary heart disease. The frequency of coronary heart disease varied widely both in different countries and in different towns in the same country. Considerable variations were found among the various towns in the frequency of stenosis and thrombosis in those who had died of coronary heart disease. This finding indicates that although atherosclerosis is indeed a prerequisite for the development of myocardial infarction, other factors may play a significant role in its occurrence. The weight of the heart in persons (excluding hypertensives) with coronary stenosis or a first fresh myocardial infarction was considerably greater than that in the low atherosclerosis group.", "contents": "Atherosclerosis of the aorta and coronary arteries in coronary heart disease. Aortic and coronary atherosclerosis and the prevalence of coronary stenosis and thrombosis were studied in subjects who had died of fresh or recurrent myocardial infarction or had suffered from myocardial infarction in the past. In general, severe atherosclerosis of the coronary arteries with stenosis and calcification was almost a prerequisite for the development of coronary heart disease. The frequency of coronary heart disease varied widely both in different countries and in different towns in the same country. Considerable variations were found among the various towns in the frequency of stenosis and thrombosis in those who had died of coronary heart disease. This finding indicates that although atherosclerosis is indeed a prerequisite for the development of myocardial infarction, other factors may play a significant role in its occurrence. The weight of the heart in persons (excluding hypertensives) with coronary stenosis or a first fresh myocardial infarction was considerably greater than that in the low atherosclerosis group."}
{"id": "PMID:1087201", "title": "Atherosclerosis and myocardial lesions in subjects dying from fresh cerebrovascular disease.", "content": "TAKING INTO ACCOUNT AGE, SEX, GEOGRAPHICAL DISTRIBUTION, OBESITY, AND ASSOCIATED CAUSE OF DEATH, IT WAS CONCLUDED THAT: (1) the extent of aortic calcification was much lower in cerebral haemorrhage than in cerebral infarct. In deaths due to cerebral haemorrhage aortic calcification was at about the same level as in those due to cancer of the stomach, while in deaths due to cerebral infarct it was at the same level as in those due to coronary heart disease; (2) the prevalence of large myocardial scar was low in deaths due to cerebral haemorrhage (at about the same level as in those due to prostatic cancer), while in deaths due to cerebral infarct it was more frequent (at the same level as in deaths due to diabetes); (3) the extent of coronary calcification and prevalence of coronary stenosis and fresh myocardial infarction were low in the cerebral haemorrhage and cerebral infarct deaths, but a little lower in the former; and (4) the extent of raised lesions of the aorta and coronary arteries was similar in the cerebral haemorrhage and cerebral infarct deaths, the level of aortic lesions being high and overlapping in level with those in the coronary heart disease and hypertensive deaths, and the levels of coronary lesions being much lower and well below those in the coronary and diabetic hypertensive deaths.", "contents": "Atherosclerosis and myocardial lesions in subjects dying from fresh cerebrovascular disease. TAKING INTO ACCOUNT AGE, SEX, GEOGRAPHICAL DISTRIBUTION, OBESITY, AND ASSOCIATED CAUSE OF DEATH, IT WAS CONCLUDED THAT: (1) the extent of aortic calcification was much lower in cerebral haemorrhage than in cerebral infarct. In deaths due to cerebral haemorrhage aortic calcification was at about the same level as in those due to cancer of the stomach, while in deaths due to cerebral infarct it was at the same level as in those due to coronary heart disease; (2) the prevalence of large myocardial scar was low in deaths due to cerebral haemorrhage (at about the same level as in those due to prostatic cancer), while in deaths due to cerebral infarct it was more frequent (at the same level as in deaths due to diabetes); (3) the extent of coronary calcification and prevalence of coronary stenosis and fresh myocardial infarction were low in the cerebral haemorrhage and cerebral infarct deaths, but a little lower in the former; and (4) the extent of raised lesions of the aorta and coronary arteries was similar in the cerebral haemorrhage and cerebral infarct deaths, the level of aortic lesions being high and overlapping in level with those in the coronary heart disease and hypertensive deaths, and the levels of coronary lesions being much lower and well below those in the coronary and diabetic hypertensive deaths."}
{"id": "PMID:1087202", "title": "Aortic, coronary, and myocardial lesions in relation to various factors.", "content": "The relative extent of raised and calcified coronary aortic lesions, prevalence of coronary stenosis and of fresh and old myocardial infarction, and mean heart weight were expressed on a continuous scale for 4 disease groups (coronary heart disease, cerebral infarction/haemorrhage, hypertension/diabetes, cancer). Within these groups the relative position for each of the lesions was calculated by subgroups of disease in order to show the elevating or depressing effect of hypertension, diabetes, obesity, and combinations of disease. The strength of association between 33 factors (town 5, disease 24, obesity, heart weight, age, and sex) and the lesions was calculated. An analysis of variance was carried out and the proportion of the variance of the different lesions accounted for by town, disease, etc., is shown. The extent of raised aortic lesions is strongly associated with age and hypertension. It is positively associated with coronary heart disease and inversely associated with cancer. Town factors have a small positive association which is larger than that due to obesity. 33 factors taken together account for 50% of the variance. Age alone accounts for 37% and sex for only 0.3%. The extent of calcified aortic lesion is strongly associated with age, town, hypertension, coronary heart disease and diabetes mellitus. It is inversely associated with cancer, more strongly in cancer of the bronchus and the liver than in other cancers. It is inversely associated with obesity. 33 factors together account for 30% of the variance and age alone accounts for about 13%. The extent of raised coronary lesions is strongly associated with age, coronary heart disease, sex, diabetes mellitus, hypertension and obesity. It is inversely associated with cancer, more strongly in cancer of the prostate and the liver than in other cancers. Town factors have a small association. 33 factors taken together account for about 43% of the variance. Age alone accounts for 25%. The extent of calcified coronary lesions is associated with coronary heart disease and age. There is a low association with hypertension and diabetes, an inverse association with cancer, and a low inverse association with obesity. 33 factors together account for 20% of the variance. Coronary heart disease alone accounts for 12% and age alone accounts for 8%. The prevalence of coronary stenosis is strongly associated with coronary heart disease, age, and sex. There is a small association with town factors, obesity, and hypertension, and an inverse association with cancer. All the factors together account for about 30% of the variance. Coronary heart disease alone accounts for 23% and age 10%.", "contents": "Aortic, coronary, and myocardial lesions in relation to various factors. The relative extent of raised and calcified coronary aortic lesions, prevalence of coronary stenosis and of fresh and old myocardial infarction, and mean heart weight were expressed on a continuous scale for 4 disease groups (coronary heart disease, cerebral infarction/haemorrhage, hypertension/diabetes, cancer). Within these groups the relative position for each of the lesions was calculated by subgroups of disease in order to show the elevating or depressing effect of hypertension, diabetes, obesity, and combinations of disease. The strength of association between 33 factors (town 5, disease 24, obesity, heart weight, age, and sex) and the lesions was calculated. An analysis of variance was carried out and the proportion of the variance of the different lesions accounted for by town, disease, etc., is shown. The extent of raised aortic lesions is strongly associated with age and hypertension. It is positively associated with coronary heart disease and inversely associated with cancer. Town factors have a small positive association which is larger than that due to obesity. 33 factors taken together account for 50% of the variance. Age alone accounts for 37% and sex for only 0.3%. The extent of calcified aortic lesion is strongly associated with age, town, hypertension, coronary heart disease and diabetes mellitus. It is inversely associated with cancer, more strongly in cancer of the bronchus and the liver than in other cancers. It is inversely associated with obesity. 33 factors together account for 30% of the variance and age alone accounts for about 13%. The extent of raised coronary lesions is strongly associated with age, coronary heart disease, sex, diabetes mellitus, hypertension and obesity. It is inversely associated with cancer, more strongly in cancer of the prostate and the liver than in other cancers. Town factors have a small association. 33 factors taken together account for about 43% of the variance. Age alone accounts for 25%. The extent of calcified coronary lesions is associated with coronary heart disease and age. There is a low association with hypertension and diabetes, an inverse association with cancer, and a low inverse association with obesity. 33 factors together account for 20% of the variance. Coronary heart disease alone accounts for 12% and age alone accounts for 8%. The prevalence of coronary stenosis is strongly associated with coronary heart disease, age, and sex. There is a small association with town factors, obesity, and hypertension, and an inverse association with cancer. All the factors together account for about 30% of the variance. Coronary heart disease alone accounts for 23% and age 10%."}
{"id": "PMID:1087203", "title": "Atherosclerosis and body build.", "content": "In a large autopsy series the relation between various measures of body build and aortic and coronary atherosclerosis, coronary stenosis, and myocardial lesions was studied. Stature was not associated with any of these variables. Various measures of obesity all showed an association between obesity and the above-mentioned variables. Obese people were found to have more coronary atherosclerosis, coronary stenosis, and myocardial lesions than thin people, a difference that persisted, in a reduced form, when hypertensives and diabetics were excluded. When compared with the standardized average atherosclerosis group to exclude the effect of \"wasting diseases\", and when hypertensives and diabetics were excluded, neither the extent of atherosclerotic lesions nor the prevalence of coronary stenosis were increased in obese subjects. Obese men but not obese women, however, had more myocardial lesions, especially fresh myocardial infarction.", "contents": "Atherosclerosis and body build. In a large autopsy series the relation between various measures of body build and aortic and coronary atherosclerosis, coronary stenosis, and myocardial lesions was studied. Stature was not associated with any of these variables. Various measures of obesity all showed an association between obesity and the above-mentioned variables. Obese people were found to have more coronary atherosclerosis, coronary stenosis, and myocardial lesions than thin people, a difference that persisted, in a reduced form, when hypertensives and diabetics were excluded. When compared with the standardized average atherosclerosis group to exclude the effect of \"wasting diseases\", and when hypertensives and diabetics were excluded, neither the extent of atherosclerotic lesions nor the prevalence of coronary stenosis were increased in obese subjects. Obese men but not obese women, however, had more myocardial lesions, especially fresh myocardial infarction."}
{"id": "PMID:1087204", "title": "Atherosclerosis and myocardial disease in relation to physical activity of occupation.", "content": "This study supports the data of other authors in finding that the proportional mortality of sudden heart death and coronary heart disease is greater in men aged 40-59 years with sedentary occupations than in those with strenuous occupations. Clear evidence was found of an association between the extent of raised coronary lesions (mainly fibrous plaque and calcified lesions in the left anterior descending coronary artery and complicated lesions in each artery) and inactivity of occupation. The evidence for a similar association with the prevalence of coronary stenosis, myocardial lesions, and the extent of raised lesions of the aorta was suggestive but less clear. There was no evidence of an association between the physical activity of occupation and the extent of calcified lesions in the aorta or coronary arteries. Prevalence and extent of atherosclerotic and myocardial lesions were compared in each of 6 cause of death groups, between those who had been in strenuous, medium, and sedentary occupations.", "contents": "Atherosclerosis and myocardial disease in relation to physical activity of occupation. This study supports the data of other authors in finding that the proportional mortality of sudden heart death and coronary heart disease is greater in men aged 40-59 years with sedentary occupations than in those with strenuous occupations. Clear evidence was found of an association between the extent of raised coronary lesions (mainly fibrous plaque and calcified lesions in the left anterior descending coronary artery and complicated lesions in each artery) and inactivity of occupation. The evidence for a similar association with the prevalence of coronary stenosis, myocardial lesions, and the extent of raised lesions of the aorta was suggestive but less clear. There was no evidence of an association between the physical activity of occupation and the extent of calcified lesions in the aorta or coronary arteries. Prevalence and extent of atherosclerotic and myocardial lesions were compared in each of 6 cause of death groups, between those who had been in strenuous, medium, and sedentary occupations."}
{"id": "PMID:1087205", "title": "Alcohol consumption and atherosclerosis.", "content": "A study was made of the material obtained in 737 autopsies carried out on men aged 30-69 years (including 88 visitors) who had died in Yalta from various diseases, accidents, and violence. Information on the consumption of alcohol was obtained by questioning the subjects' near relations, neighbours, and associates, as well as from medical records. The most reliable part of this information-the average number of days per month on which the subject consumed alcohol-was taken as the basic criterion for grouping the observations. People taking alcohol on 16 or more days per month had more extensive atherosclerotic raised lesions in the aorta than those drinking on not more than 3 days per month. This difference was most significant in the case of calcified lesions. Fatty streak in the aorta was more extensive in the medium group (drinking on 4-15 days per month) than in either of the extreme groups. As regards the coronary arteries, a significant difference positively associated with alcohol consumption was established only in the case of calcification. The few differences concerning myocardial and cerebral infarction had a negative association with alcohol consumption.", "contents": "Alcohol consumption and atherosclerosis. A study was made of the material obtained in 737 autopsies carried out on men aged 30-69 years (including 88 visitors) who had died in Yalta from various diseases, accidents, and violence. Information on the consumption of alcohol was obtained by questioning the subjects' near relations, neighbours, and associates, as well as from medical records. The most reliable part of this information-the average number of days per month on which the subject consumed alcohol-was taken as the basic criterion for grouping the observations. People taking alcohol on 16 or more days per month had more extensive atherosclerotic raised lesions in the aorta than those drinking on not more than 3 days per month. This difference was most significant in the case of calcified lesions. Fatty streak in the aorta was more extensive in the medium group (drinking on 4-15 days per month) than in either of the extreme groups. As regards the coronary arteries, a significant difference positively associated with alcohol consumption was established only in the case of calcification. The few differences concerning myocardial and cerebral infarction had a negative association with alcohol consumption."}
{"id": "PMID:1087206", "title": "Atherosclerosis in smokers.", "content": "Cigarette smoking was found to be associated with an increased extent of raised lesions of the aorta and aortic calcification. The apparent association with aortic calcification was mainly due to co-existing alcohol consumption. The apparent association of smoking with the extent of coronary calcification was also related to co-existing alcohol consumption; smoking alone tended to be negatively associated with this lesion. There was no clear association between smoking and coronary stenosis, myocardial infarction, and heart weight.", "contents": "Atherosclerosis in smokers. Cigarette smoking was found to be associated with an increased extent of raised lesions of the aorta and aortic calcification. The apparent association with aortic calcification was mainly due to co-existing alcohol consumption. The apparent association of smoking with the extent of coronary calcification was also related to co-existing alcohol consumption; smoking alone tended to be negatively associated with this lesion. There was no clear association between smoking and coronary stenosis, myocardial infarction, and heart weight."}
{"id": "PMID:1087207", "title": "Immunofixation. III. Application to the study of monoclonal proteins.", "content": "We have shown how immunofixation can be successfully used to study proteins exhibiting electrophoretic polymorphism. An analogous situation is seen in the disorders of the immune system that result in the production of variable quantitites of homogeneous immunoglobulins. Immunoelectrophroesis has been heavily used--sometimes unsuccessfully because of poor resolution--to study these materials. The appearance of a monoclonal immunoglobulin band is identical in both immunofixation and standard gel electrophoresis, a feature that allows unambiguous identification of a protein as to its heavy-chain class and light-chain type. Because antigen and antibody are placed in contact almost immediately after electrophoresis, interfering diffusion does not occur and even extremely small bands can be demonstrated and characterized.", "contents": "Immunofixation. III. Application to the study of monoclonal proteins. We have shown how immunofixation can be successfully used to study proteins exhibiting electrophoretic polymorphism. An analogous situation is seen in the disorders of the immune system that result in the production of variable quantitites of homogeneous immunoglobulins. Immunoelectrophroesis has been heavily used--sometimes unsuccessfully because of poor resolution--to study these materials. The appearance of a monoclonal immunoglobulin band is identical in both immunofixation and standard gel electrophoresis, a feature that allows unambiguous identification of a protein as to its heavy-chain class and light-chain type. Because antigen and antibody are placed in contact almost immediately after electrophoresis, interfering diffusion does not occur and even extremely small bands can be demonstrated and characterized."}
{"id": "PMID:1087209", "title": "Severe combined immunodeficiency with B lymphocytes: in vitro correction of defective immunoglobulin production by addition of normal T lymphocytes.", "content": "A 6 1/2-month-old male with severe combined immunodeficiency (SCID) had a low percentage and number of T cells (11%; 241/mm3) and a high percentage and number of B cells (52%; 1187/mm3) and null cells (37%; 868/mm3). In vitro studies were performed to determine if this child's primary defect involved differentiation of both T and B lymphocytes or if failure of B lymphocytes to differentiate into immunoglobulin producing cells was secondary to T lymphocyte abnormalities. Immunoglobulin production by lymphocytes in response to polyclonal mitogens (pokeweed mitogen and foetal calf serum) was measured by pulse-labelling cells with 3H-leucine and then precipitating cytoplasmic and secreted immunoglobulins with polyvalent anti-human immunoglobulin and S. aureus (Cowan strain I) protein A. The patient's lymphocytes did not synthesize immunoglobulins in vitro in response to mitogens. They did not suppress synthesis of immunoglobulins by normal lymphocytes. However, addition of normal purified T cells, which themselves did not synthesize immunoglobulins, enabled the patient's B lymphocytes to become immunoglobulin synthesizing and secreting cells. Gamma, mu, and light chains were secreted. This suggests that the primary abnormality was in the T-cell axis at the level of lymphoid stem cells or prothymocytes and that failure of B lymphocytes to become immunoglobulin-producing cells was secondary to this defect.", "contents": "Severe combined immunodeficiency with B lymphocytes: in vitro correction of defective immunoglobulin production by addition of normal T lymphocytes. A 6 1/2-month-old male with severe combined immunodeficiency (SCID) had a low percentage and number of T cells (11%; 241/mm3) and a high percentage and number of B cells (52%; 1187/mm3) and null cells (37%; 868/mm3). In vitro studies were performed to determine if this child's primary defect involved differentiation of both T and B lymphocytes or if failure of B lymphocytes to differentiate into immunoglobulin producing cells was secondary to T lymphocyte abnormalities. Immunoglobulin production by lymphocytes in response to polyclonal mitogens (pokeweed mitogen and foetal calf serum) was measured by pulse-labelling cells with 3H-leucine and then precipitating cytoplasmic and secreted immunoglobulins with polyvalent anti-human immunoglobulin and S. aureus (Cowan strain I) protein A. The patient's lymphocytes did not synthesize immunoglobulins in vitro in response to mitogens. They did not suppress synthesis of immunoglobulins by normal lymphocytes. However, addition of normal purified T cells, which themselves did not synthesize immunoglobulins, enabled the patient's B lymphocytes to become immunoglobulin synthesizing and secreting cells. Gamma, mu, and light chains were secreted. This suggests that the primary abnormality was in the T-cell axis at the level of lymphoid stem cells or prothymocytes and that failure of B lymphocytes to become immunoglobulin-producing cells was secondary to this defect."}
{"id": "PMID:1087210", "title": "The effects of purified mitogenic proteins (Pa-1 and Pa-2) from pokeweed on human T and B lymphocytes in vitro.", "content": "Purified proteins (Pa-1 and Pa-2) from pokeweed have been compared with commercial pokeweed mitogen (PWM-G) and other mitogens in their ability to stimulate human lymphocytes. With cultures of T and B cells separated from tonsil lymphocytes, thymidine uptake, blast transformation and immunoglobulin (Ig) synthesis have been measured. IgM and IgG was measured in supernates of stimulated cultures by radioimmunoassay. Pa-1, Pa-2 and PWM-G were found to be potent mitogens for unseparated tonsil lymphocytes or nylon column purified T cells. Pa-2 was found to be active at lower concentrations than Pa-1, and PWM-G was less potent than the purified mitogens. These three mitogens all stimulated unseparated lymphocytes to secrete large quantities of Ig (20-100 mug/ml) during 7 days in culture. With increasing amounts of mitogens severe decreases in immunoglobulin synthesis were observed at day 6 even with doses which were still optimal for stimulation of thymidine uptake at days 3 and 6. With purified B cells (less than 2% T cells) Pa-1 was the best mitogen for thymidine incorporation. However, the secretory response was very variable. In some experiments B cells did not secrete Ig in response to mitogens; in others Pa-1 was clearly more effective at stimulating secretion than Pa-2 or PWM-G and in some experiments B cells were stimulated by all three. In one experiment Pa-1 stimulated prolymphocytic leukaemia cells to blast transformation and the secretion of IgM. It is concluded that Pa-1, Pa-2 and PWM-G are much better activators of Ig synthesis in human cultures than either PHA or LPS and that Pa-1 is the most reliable B-cell stimulant of the three.", "contents": "The effects of purified mitogenic proteins (Pa-1 and Pa-2) from pokeweed on human T and B lymphocytes in vitro. Purified proteins (Pa-1 and Pa-2) from pokeweed have been compared with commercial pokeweed mitogen (PWM-G) and other mitogens in their ability to stimulate human lymphocytes. With cultures of T and B cells separated from tonsil lymphocytes, thymidine uptake, blast transformation and immunoglobulin (Ig) synthesis have been measured. IgM and IgG was measured in supernates of stimulated cultures by radioimmunoassay. Pa-1, Pa-2 and PWM-G were found to be potent mitogens for unseparated tonsil lymphocytes or nylon column purified T cells. Pa-2 was found to be active at lower concentrations than Pa-1, and PWM-G was less potent than the purified mitogens. These three mitogens all stimulated unseparated lymphocytes to secrete large quantities of Ig (20-100 mug/ml) during 7 days in culture. With increasing amounts of mitogens severe decreases in immunoglobulin synthesis were observed at day 6 even with doses which were still optimal for stimulation of thymidine uptake at days 3 and 6. With purified B cells (less than 2% T cells) Pa-1 was the best mitogen for thymidine incorporation. However, the secretory response was very variable. In some experiments B cells did not secrete Ig in response to mitogens; in others Pa-1 was clearly more effective at stimulating secretion than Pa-2 or PWM-G and in some experiments B cells were stimulated by all three. In one experiment Pa-1 stimulated prolymphocytic leukaemia cells to blast transformation and the secretion of IgM. It is concluded that Pa-1, Pa-2 and PWM-G are much better activators of Ig synthesis in human cultures than either PHA or LPS and that Pa-1 is the most reliable B-cell stimulant of the three."}
{"id": "PMID:1087211", "title": "Lymphocyte subpopulation in neoplastic and non-neoplastic thymus and in blood of patients with Myasthenia gravis.", "content": "The lymphocyte subpopulations in the thymus and in the blood were investigated in ten myasthenic patients who had been thymectomized. Histologically, the thymuses tested comprised three cases of thymoma including two cases with malignant characteristics, five cases of hyperplastic thymus with lymph follicles and germinal centres, and two cases of persistent thymus without lymph follicles. Virtually all lymphoid cells in the three thymomas formed spontaneous rosettes with sheep red blood cells as did normal thymocytes from non-myasthenic patients. There was no significant proportion of immunoglobulin Ig-bearing lymphocytes. While the majority consisted of cells forming spontaneous rosettes with sheep red blood cells, there was a certain proportion (2-17%) of Ig-bearing lymphocytes in four of five hyperplastic thymuses, in one of two persistent thymuses, and in a residual atrophic thymus of a thymoma. The myasthenic patients tested were for the most part normal, as compared with healthy individuals, in the proportion of rosette-forming lymphocytes and Ig-bearing lymphocytes in the blood collected immediately before and one to three months after thymectomy. The presence of Ig-bearing lymphocytes in the thymus was not necessarily related to the appearance of circulating antibody to striated muscle. The antibody to striated muscle was demonstrated in all myasthenic patients with thymoma.", "contents": "Lymphocyte subpopulation in neoplastic and non-neoplastic thymus and in blood of patients with Myasthenia gravis. The lymphocyte subpopulations in the thymus and in the blood were investigated in ten myasthenic patients who had been thymectomized. Histologically, the thymuses tested comprised three cases of thymoma including two cases with malignant characteristics, five cases of hyperplastic thymus with lymph follicles and germinal centres, and two cases of persistent thymus without lymph follicles. Virtually all lymphoid cells in the three thymomas formed spontaneous rosettes with sheep red blood cells as did normal thymocytes from non-myasthenic patients. There was no significant proportion of immunoglobulin Ig-bearing lymphocytes. While the majority consisted of cells forming spontaneous rosettes with sheep red blood cells, there was a certain proportion (2-17%) of Ig-bearing lymphocytes in four of five hyperplastic thymuses, in one of two persistent thymuses, and in a residual atrophic thymus of a thymoma. The myasthenic patients tested were for the most part normal, as compared with healthy individuals, in the proportion of rosette-forming lymphocytes and Ig-bearing lymphocytes in the blood collected immediately before and one to three months after thymectomy. The presence of Ig-bearing lymphocytes in the thymus was not necessarily related to the appearance of circulating antibody to striated muscle. The antibody to striated muscle was demonstrated in all myasthenic patients with thymoma."}
{"id": "PMID:1087212", "title": "Immune status and BCG vaccination in newborns with intra-uterine growth retardation.", "content": "In severe intra-uterine growth retardation there are low IgG levels, low numbers of B cells and low percentage as well as numbers of T cells in cord blood. Following BCG vaccination given soon after birth, Mantoux conversion and leucocyte migration inhibition response to BCG was defective.", "contents": "Immune status and BCG vaccination in newborns with intra-uterine growth retardation. In severe intra-uterine growth retardation there are low IgG levels, low numbers of B cells and low percentage as well as numbers of T cells in cord blood. Following BCG vaccination given soon after birth, Mantoux conversion and leucocyte migration inhibition response to BCG was defective."}
{"id": "PMID:1087213", "title": "Lymphocytotoxins in acute and chronic hepatitis. Characterization and relationship to changes in circulating T lymphocytes.", "content": "Serum lymphocytotoxicity (LCT) was detected in 49% of fifty-one patients with acute viral hepatitis and 72% of twenty-nine patients with chronic hepatitis. LCT was not detected in ten chronic carriers of hepatitis B surface antigen. Characterization of LCT revealed it to be active at physiologic temperatures and to be reactive against both T and B lymphocytes. The occurrence of LCT was transient in acute hepatitis and intermittent in chronic hepatitis. There was a significant inverse relationship between the percentage change in LCT over time and peripheral blood T-cell proportions amongst the patients studied. These findings indicate the importance of liver damage in the appearance of LCT and suggest that LCT may contribute to depressed lymphocyte function in liver disease.", "contents": "Lymphocytotoxins in acute and chronic hepatitis. Characterization and relationship to changes in circulating T lymphocytes. Serum lymphocytotoxicity (LCT) was detected in 49% of fifty-one patients with acute viral hepatitis and 72% of twenty-nine patients with chronic hepatitis. LCT was not detected in ten chronic carriers of hepatitis B surface antigen. Characterization of LCT revealed it to be active at physiologic temperatures and to be reactive against both T and B lymphocytes. The occurrence of LCT was transient in acute hepatitis and intermittent in chronic hepatitis. There was a significant inverse relationship between the percentage change in LCT over time and peripheral blood T-cell proportions amongst the patients studied. These findings indicate the importance of liver damage in the appearance of LCT and suggest that LCT may contribute to depressed lymphocyte function in liver disease."}
{"id": "PMID:1087214", "title": "Double-stranded DNA-binding capacity of serum in acute and chronic liver disease.", "content": "Serum antibodies to double-stranded 'native' DNA have been measured in acute and chronic liver diseases using the Farr technique. Elevated levels of DNA binding were found in all groups of patients, with the highest levels in acute viral hepatitis and lowest in primary biliary cirrhosis. All patients with hepatitis B surface antigen-positive chronic active hepatitis had elevated levels, hence persistent elevation of DNA binding after acute type B hepatitis might be an unfavourable prognostic marker indicating progression to chronic active hepatitis, DNA antibody levels will not offer diagnostic help in liver diseases, or help to follow the response of patients with 'lupoid' hepatitis to corticosteroid therapy. Production of DNA antibody may be a response to release of DNA from damaged hepatocytes.", "contents": "Double-stranded DNA-binding capacity of serum in acute and chronic liver disease. Serum antibodies to double-stranded 'native' DNA have been measured in acute and chronic liver diseases using the Farr technique. Elevated levels of DNA binding were found in all groups of patients, with the highest levels in acute viral hepatitis and lowest in primary biliary cirrhosis. All patients with hepatitis B surface antigen-positive chronic active hepatitis had elevated levels, hence persistent elevation of DNA binding after acute type B hepatitis might be an unfavourable prognostic marker indicating progression to chronic active hepatitis, DNA antibody levels will not offer diagnostic help in liver diseases, or help to follow the response of patients with 'lupoid' hepatitis to corticosteroid therapy. Production of DNA antibody may be a response to release of DNA from damaged hepatocytes."}
{"id": "PMID:1087215", "title": "A subpopulation of human B lymphocytes that rosette with mouse erythrocytes.", "content": "A proportion of human peripheral blood lymphocytes form rosettes with mouse erythrocytes (M-RFC). It is confirmed that the proportion of such rosette-forming cells is high in chronic lymphocytic leukaemia (CLL). Analysis of normal lymphocyte populations revealed that M-RFC belong to the B-lymphocyte subclass exclusively. Analysis of their surface markers showed: (a) complement receptors in 50% as compared to 71% of the total B-cell population; (b) a distribution of surface immunoglobulins G, A, M and E typical of the lymphocyte sources; (c) lack of sheep erythrocyte receptor. No differences in the ratio of M-RFC to total B cells was found between lymphocyte population from tonsils, bone marrow and peripheral blood although a significantly higher ratio was seen in cord blood and in chronic lymphocytic leukaemia. Investigation of the properties of mouse erythrocyte rosette formation revealed the following: (a) incubation of lymphocyte mouse erythrocyte mixtures at 37degreesC before centrifugation inhibited rosette formation when CLL lymphocytes were used; (b) treatment of mouse erythrocytes with neuraminidase or trypsin increased their adhesiveness to lymphocytes; (c) treatment of lymphocytes with neuraminidase promoted M-rosette formation but trypsin treatment had an inhibitory effect; (d) cyanide and fluoride at concentrations which strongly inhibited E-rosette formation had no inhibitory effect on M rosettes; (e) M-rosette formation was inhibited by anti-immunoglobulin serum but not by anti-lymphocyte serum; and (f) M-rosette formation was also inhibited by the presence of staphylococci. E-rosette formation was unaffected. The nature of the bond in mouse rosettes is discussed in the light of these findings. The evidence indicates that the lymphocyte receptor may be a part of an immunoglobulin molecule.", "contents": "A subpopulation of human B lymphocytes that rosette with mouse erythrocytes. A proportion of human peripheral blood lymphocytes form rosettes with mouse erythrocytes (M-RFC). It is confirmed that the proportion of such rosette-forming cells is high in chronic lymphocytic leukaemia (CLL). Analysis of normal lymphocyte populations revealed that M-RFC belong to the B-lymphocyte subclass exclusively. Analysis of their surface markers showed: (a) complement receptors in 50% as compared to 71% of the total B-cell population; (b) a distribution of surface immunoglobulins G, A, M and E typical of the lymphocyte sources; (c) lack of sheep erythrocyte receptor. No differences in the ratio of M-RFC to total B cells was found between lymphocyte population from tonsils, bone marrow and peripheral blood although a significantly higher ratio was seen in cord blood and in chronic lymphocytic leukaemia. Investigation of the properties of mouse erythrocyte rosette formation revealed the following: (a) incubation of lymphocyte mouse erythrocyte mixtures at 37degreesC before centrifugation inhibited rosette formation when CLL lymphocytes were used; (b) treatment of mouse erythrocytes with neuraminidase or trypsin increased their adhesiveness to lymphocytes; (c) treatment of lymphocytes with neuraminidase promoted M-rosette formation but trypsin treatment had an inhibitory effect; (d) cyanide and fluoride at concentrations which strongly inhibited E-rosette formation had no inhibitory effect on M rosettes; (e) M-rosette formation was inhibited by anti-immunoglobulin serum but not by anti-lymphocyte serum; and (f) M-rosette formation was also inhibited by the presence of staphylococci. E-rosette formation was unaffected. The nature of the bond in mouse rosettes is discussed in the light of these findings. The evidence indicates that the lymphocyte receptor may be a part of an immunoglobulin molecule."}
{"id": "PMID:1087217", "title": "Clinical reliability of posterior fossa scintigraphy.", "content": "Radioisotope imaging of the brain is considerably less useful in posterior fossa lesions than in supratentorial diagnosis, due to the peculiar technical difficulties and biological characteristics of infratentorial lesions. Exceptions are acoustic neurofibromas and probably meningiomas, which can be reliably detected. The importance is stressed of making skull and chest radiographs at the time of the examination, in order to enhance the diagnostic accuracy of the scintigraphic result.", "contents": "Clinical reliability of posterior fossa scintigraphy. Radioisotope imaging of the brain is considerably less useful in posterior fossa lesions than in supratentorial diagnosis, due to the peculiar technical difficulties and biological characteristics of infratentorial lesions. Exceptions are acoustic neurofibromas and probably meningiomas, which can be reliably detected. The importance is stressed of making skull and chest radiographs at the time of the examination, in order to enhance the diagnostic accuracy of the scintigraphic result."}
{"id": "PMID:1087218", "title": "Timing of permanent tooth emergence in two Greenland Eskimo populations.", "content": "Mean times of emergence of the permanent teeth (except the first and third molars and mandibular central incisors) were studied cross-sectionally in an unmixed East Greenland population in 1957 and in present West Greenland Eskimo children. In spite of differences in racial and environmental conditions, practically no difference was found between the two samples in the timing of dental development in general, taking into account the average difference in emergence times of the individual teeth. Both samples showed advanced dental development in comparison with two unmixed Caucasoid and two unmixed Mongoloid populations. The mutual similarity in timing of tooth emergence between the East and West Greenland Eskimo children considerably greater than the similarity between each of the Eskimo samples and any of the other four populations.", "contents": "Timing of permanent tooth emergence in two Greenland Eskimo populations. Mean times of emergence of the permanent teeth (except the first and third molars and mandibular central incisors) were studied cross-sectionally in an unmixed East Greenland population in 1957 and in present West Greenland Eskimo children. In spite of differences in racial and environmental conditions, practically no difference was found between the two samples in the timing of dental development in general, taking into account the average difference in emergence times of the individual teeth. Both samples showed advanced dental development in comparison with two unmixed Caucasoid and two unmixed Mongoloid populations. The mutual similarity in timing of tooth emergence between the East and West Greenland Eskimo children considerably greater than the similarity between each of the Eskimo samples and any of the other four populations."}
{"id": "PMID:1087221", "title": "Anomalous origin of left coronary artery from pulmonary artery.", "content": "An anomalous origin of the left coronary artery from the pulmonary artery in a 26-year-old man was corrected by the ligation of the artery at its anomalous origin, followed by the construction of an aortocoronary bypass with a venous graft. The flow of blood to the myocardium was measured for the purpose of comparing the effect of the bypass method with that of the ligation method. The result suggested that the bypass method provided a greater flow of blood to the myocardium than did ligation alone.", "contents": "Anomalous origin of left coronary artery from pulmonary artery. An anomalous origin of the left coronary artery from the pulmonary artery in a 26-year-old man was corrected by the ligation of the artery at its anomalous origin, followed by the construction of an aortocoronary bypass with a venous graft. The flow of blood to the myocardium was measured for the purpose of comparing the effect of the bypass method with that of the ligation method. The result suggested that the bypass method provided a greater flow of blood to the myocardium than did ligation alone."}
{"id": "PMID:1087224", "title": "Participation of thyroid D-aspartate oxidase in iodide oxidation and incorporation into thyroid proteins.", "content": "It was found that the H2O2 generating system containing D-aspartate oxidase isolated from the thyroid gland and D-aspartate, takes part in oxidation of iodides. The molecular I2 formed under experimental conditions is subsequently incorporated into thyroid proteins. Thiosemicarbazide, thiourea, methylthiouracyl, sulphathiazole, thiocyanate and azides were found to have an inhibiting effect on iodide oxidation. Methimazole inhibits both the oxidation of iodide and incorporation of 131I into protein. The iodide incorporation was inhibited by catalase. The findings of these investigations suggest an indirect participation of D-aspartate oxidase in the synthesis of the thyroid hormone by supplying the essential substrate for the iodide oxidation, H2O2.", "contents": "Participation of thyroid D-aspartate oxidase in iodide oxidation and incorporation into thyroid proteins. It was found that the H2O2 generating system containing D-aspartate oxidase isolated from the thyroid gland and D-aspartate, takes part in oxidation of iodides. The molecular I2 formed under experimental conditions is subsequently incorporated into thyroid proteins. Thiosemicarbazide, thiourea, methylthiouracyl, sulphathiazole, thiocyanate and azides were found to have an inhibiting effect on iodide oxidation. Methimazole inhibits both the oxidation of iodide and incorporation of 131I into protein. The iodide incorporation was inhibited by catalase. The findings of these investigations suggest an indirect participation of D-aspartate oxidase in the synthesis of the thyroid hormone by supplying the essential substrate for the iodide oxidation, H2O2."}
{"id": "PMID:1087225", "title": "Re-evaluation of the role of aldosterone in mechanism of natriuresis induced by extracellular fluid volume expansion.", "content": "Acute experiments were performed in thiobarbiturate (Inactin, PROMONTA) anaesthesia in 21 male rats. It was found that the attenuation of the antinatriuretic effect of aldosterone by a spironolactone Aldactone (BOEHRINGER) in a dose of 10 mg per rat in 1 h i.v. enhanced urine (V) and sodium (UNaV) excretion following the extracellular fluid volume expansion (ECFV) with intravenous infusion of saline. Max. V = 81.44 +/- +/- 7.46 mul min-1 was obtained in Aldactone treated rats as compared to 54.95 + 7.24 mul min-1 in controls: UNaV = 9.89 +/- 1.26 muval min-1 vs. 6.17 +/- 0.73 muval min-1, respectively. The urinary Na/K ratio was also significantly higher in the same samples of Aldactone treated rats (6.15 +/- +/- 1.22 vs. 2.68 +/- 0.19). It is concluded that endogenous aldosterone blunts nriuresis induced by the ECFV expansion.", "contents": "Re-evaluation of the role of aldosterone in mechanism of natriuresis induced by extracellular fluid volume expansion. Acute experiments were performed in thiobarbiturate (Inactin, PROMONTA) anaesthesia in 21 male rats. It was found that the attenuation of the antinatriuretic effect of aldosterone by a spironolactone Aldactone (BOEHRINGER) in a dose of 10 mg per rat in 1 h i.v. enhanced urine (V) and sodium (UNaV) excretion following the extracellular fluid volume expansion (ECFV) with intravenous infusion of saline. Max. V = 81.44 +/- +/- 7.46 mul min-1 was obtained in Aldactone treated rats as compared to 54.95 + 7.24 mul min-1 in controls: UNaV = 9.89 +/- 1.26 muval min-1 vs. 6.17 +/- 0.73 muval min-1, respectively. The urinary Na/K ratio was also significantly higher in the same samples of Aldactone treated rats (6.15 +/- +/- 1.22 vs. 2.68 +/- 0.19). It is concluded that endogenous aldosterone blunts nriuresis induced by the ECFV expansion."}
{"id": "PMID:1087226", "title": "Inactivation of Oxytocin and its analogues by subcellular fractions of hen tissues.", "content": "The enzymic inactivation of oxytocin by liver, kidney, uterus and pancreas homogenate subcellular fractions of hens was studied. Oxytocin was most rapidly degraded by the soluble fraction of tissues examined. All the subcellular fractions of liver and kidney inactivated oxytocin, but only the microsomal and soluble fractions of uterus and pancreas showed the oxytocin-inactivating activity. The location of enzymes inactivating oxytocin in subcellular fractions of hen tissues was investigated with the aid of synthetic analogues of oxytocin (deamino-oxytocin and deamino-carba1-oxytocin). The carboxamidopeptidase activity, hydrolyzing the amide bonds in the linear portion of oxytocin was located in the soluble fraction of hen liver, kidney and uterus. No carboxamidopeptidase activity in the pancreatic soluble fraction was found. These results showed that aminopeptidase activity is bound to heavy subcellular particles in the hen tissue. An action of unknown endopeptidases was observed in the microsomal fraction of uterus and pancreas.", "contents": "Inactivation of Oxytocin and its analogues by subcellular fractions of hen tissues. The enzymic inactivation of oxytocin by liver, kidney, uterus and pancreas homogenate subcellular fractions of hens was studied. Oxytocin was most rapidly degraded by the soluble fraction of tissues examined. All the subcellular fractions of liver and kidney inactivated oxytocin, but only the microsomal and soluble fractions of uterus and pancreas showed the oxytocin-inactivating activity. The location of enzymes inactivating oxytocin in subcellular fractions of hen tissues was investigated with the aid of synthetic analogues of oxytocin (deamino-oxytocin and deamino-carba1-oxytocin). The carboxamidopeptidase activity, hydrolyzing the amide bonds in the linear portion of oxytocin was located in the soluble fraction of hen liver, kidney and uterus. No carboxamidopeptidase activity in the pancreatic soluble fraction was found. These results showed that aminopeptidase activity is bound to heavy subcellular particles in the hen tissue. An action of unknown endopeptidases was observed in the microsomal fraction of uterus and pancreas."}
{"id": "PMID:1087227", "title": "Thyroparathyroid conditioning of hydrocortisone and glucagon-induced hypocalcemic effect in rats.", "content": "A single dose of 2.5 mg hydrocortisone induced hypocalcemia in both intact and parathyroidectomized rats. This effect disappeared in the thyroparathyroidectomized group and did not reappear after the substitution with parathormone. A single dose of 0.2 mg glucagon produced a significant hypocalcemic effect in intact, but not in parathyroidectomized rats. In the latter group, however, the hypocalcemic effect of glucagon reappeared after the administration of parathormone. It was concluded that the hypocalcemic effect of hydrocortisone may be conditioned by the presence of calcitonin secreting thyroid and that of glucagon - by the presence of parathyroids.", "contents": "Thyroparathyroid conditioning of hydrocortisone and glucagon-induced hypocalcemic effect in rats. A single dose of 2.5 mg hydrocortisone induced hypocalcemia in both intact and parathyroidectomized rats. This effect disappeared in the thyroparathyroidectomized group and did not reappear after the substitution with parathormone. A single dose of 0.2 mg glucagon produced a significant hypocalcemic effect in intact, but not in parathyroidectomized rats. In the latter group, however, the hypocalcemic effect of glucagon reappeared after the administration of parathormone. It was concluded that the hypocalcemic effect of hydrocortisone may be conditioned by the presence of calcitonin secreting thyroid and that of glucagon - by the presence of parathyroids."}
{"id": "PMID:1087228", "title": "Analysis of variables in treatment of anovulation with human gonadotrophins.", "content": "Twenty seven women with anovulation were given 121 courses of gonadotrophins monitored by excretion of estrogen and pregnanediol. The FSH, ovulating dosage of HCG, time interval between FSH and HCG and subsequent supporting doses of HCG were varied in a statistical design. None significantly affected ovulation or pregnancy rates. The preparation with an FSH:LH ratio of 1 required a lower dosage to initiate an estrogen response than that with a ratio of 5. After adjustment it still produced a faster rise in estrogen excretion. The amount of estrogen also varied with the time interval between FSH and HCG, the ovulating dose of HCG and supporting doses of HCG. The amount of pregnanediol varied with the ovulating dose of HCG and the length of the luteal phase varied with the ovulating dose of HCG and supporting injections of HCG. The dosage of FSH needed to initiate a response increased on average by 8% from one course to the next. Simplicity, economy and risk of multiple pregnancy are discussed in relation to these variables.", "contents": "Analysis of variables in treatment of anovulation with human gonadotrophins. Twenty seven women with anovulation were given 121 courses of gonadotrophins monitored by excretion of estrogen and pregnanediol. The FSH, ovulating dosage of HCG, time interval between FSH and HCG and subsequent supporting doses of HCG were varied in a statistical design. None significantly affected ovulation or pregnancy rates. The preparation with an FSH:LH ratio of 1 required a lower dosage to initiate an estrogen response than that with a ratio of 5. After adjustment it still produced a faster rise in estrogen excretion. The amount of estrogen also varied with the time interval between FSH and HCG, the ovulating dose of HCG and supporting doses of HCG. The amount of pregnanediol varied with the ovulating dose of HCG and the length of the luteal phase varied with the ovulating dose of HCG and supporting injections of HCG. The dosage of FSH needed to initiate a response increased on average by 8% from one course to the next. Simplicity, economy and risk of multiple pregnancy are discussed in relation to these variables."}
{"id": "PMID:1087229", "title": "Plasma oxytocin and oxytocinase levels in third trimester of pregnancy and at labour.", "content": "In women during the third trimester of normal pregnancy and at labour the level of oxytocin and progesterone in plasma were determined by means of radioimmunoassay and the activity of oxytocinase was measured spectrophotometrically. The plasma oxytocin levels measured from the 25th week of pregnancy showed a gradual increase with the peak values at the 39th and 40th week. At labour, the oxytocin values remain at the same level as these found at the end of pregnancy. The increase in oxytocin levels at contractions compared to its values found between contractions was not statistically significant. Oxytocinase levels showed also an ascending character until the 38th week of pregnancy. Later, a decrease was found which continued until the termination of pregnancy. At labour, there was a decrease in progesterone level in plasma which was about at the limit of significance.", "contents": "Plasma oxytocin and oxytocinase levels in third trimester of pregnancy and at labour. In women during the third trimester of normal pregnancy and at labour the level of oxytocin and progesterone in plasma were determined by means of radioimmunoassay and the activity of oxytocinase was measured spectrophotometrically. The plasma oxytocin levels measured from the 25th week of pregnancy showed a gradual increase with the peak values at the 39th and 40th week. At labour, the oxytocin values remain at the same level as these found at the end of pregnancy. The increase in oxytocin levels at contractions compared to its values found between contractions was not statistically significant. Oxytocinase levels showed also an ascending character until the 38th week of pregnancy. Later, a decrease was found which continued until the termination of pregnancy. At labour, there was a decrease in progesterone level in plasma which was about at the limit of significance."}
{"id": "PMID:1087230", "title": "Effect of long-term fluoride administration on thyroid hormones level blood in rats.", "content": "The effect of 2 months fluoride administration (0.1 and 1.0 mg daily/rat) on thyroxine and triiodothyronine level in blood and T3-resin uptake ratio was investigated in rats. In addition, free thyroxine index was calculated from serum thyroxine level and T3-resin uptake ratio. It was found that fluoride administration caused: 1. decrease in thyroxine and triiodothyronine level in plasma; 2. decrease in free thyroxine index values; 3. Increase in T3-resin uptake ratio. From these observations as well as from previous experiments it was concluded that fluoride given continuously to the rats may influence the thyroid gland rather indirectly by changing thyroid hormone transportation in the blood.", "contents": "Effect of long-term fluoride administration on thyroid hormones level blood in rats. The effect of 2 months fluoride administration (0.1 and 1.0 mg daily/rat) on thyroxine and triiodothyronine level in blood and T3-resin uptake ratio was investigated in rats. In addition, free thyroxine index was calculated from serum thyroxine level and T3-resin uptake ratio. It was found that fluoride administration caused: 1. decrease in thyroxine and triiodothyronine level in plasma; 2. decrease in free thyroxine index values; 3. Increase in T3-resin uptake ratio. From these observations as well as from previous experiments it was concluded that fluoride given continuously to the rats may influence the thyroid gland rather indirectly by changing thyroid hormone transportation in the blood."}
{"id": "PMID:1087231", "title": "Species specificity of guinea pig erythropoietin.", "content": "Various stimuli (hypoxic hypoxia, bleeding, carbon monoxide hypoxia and cobalt administration), well known to increase erythropoietin plasma level in many species including man, failed to increase the erythropoietin activity in plasma of guinea pigs when measured in the polycythaemic mice assay. Nevertheless, the plasma of these guinea pigs stimulated the erythropoiesis in polycythaemic guinea pigs which, however, failed to respond to rat erythropoietin. From this it was concluded that not only the erythropoietin, but also the erythropoietin responsive cells in the bone marrow of guinea pigs are species specific. Bilateral nephrectomy in guinea pigs exposed to hypoxia prevented the increase in the erythropoietin plasma level, thus suggesting that, similarly as in other species, also in guinea pigs the kidney is the main organ of erythropoietin elaboration. The species specificity of guinea pig erythropoietin - erythropoiesis system is an interesting exception among mammals, since the erythropoietin of a large number of species belonging to this class was demonstrated to lack species specificity.", "contents": "Species specificity of guinea pig erythropoietin. Various stimuli (hypoxic hypoxia, bleeding, carbon monoxide hypoxia and cobalt administration), well known to increase erythropoietin plasma level in many species including man, failed to increase the erythropoietin activity in plasma of guinea pigs when measured in the polycythaemic mice assay. Nevertheless, the plasma of these guinea pigs stimulated the erythropoiesis in polycythaemic guinea pigs which, however, failed to respond to rat erythropoietin. From this it was concluded that not only the erythropoietin, but also the erythropoietin responsive cells in the bone marrow of guinea pigs are species specific. Bilateral nephrectomy in guinea pigs exposed to hypoxia prevented the increase in the erythropoietin plasma level, thus suggesting that, similarly as in other species, also in guinea pigs the kidney is the main organ of erythropoietin elaboration. The species specificity of guinea pig erythropoietin - erythropoiesis system is an interesting exception among mammals, since the erythropoietin of a large number of species belonging to this class was demonstrated to lack species specificity."}
{"id": "PMID:1087232", "title": "Influence of different cytotoxic drugs on T and B lymphocytes in children with all during remission maintenance therapy.", "content": "The influence of four different cytotoxic drugs (MTX, CYC, 6MP, and ARA-C) on T, B, and O-lymphocytes was investigated in 20 children with ALL in complete remission during cyclic remission maintenance therapy. Each of the four drugs causes a marked reduction of the absolute number of T and B cells whereas the relative number lies within the normal range with the exception of CYC, which leads to a depression of the percentage of both T and B cells. The percentage of O cells is markedly increased by CYC and slightly increased by MTX, 6MP, and ARA-C. The data are interpreted with care since the function of the immune system and especially tumor rejection depends on the interaction between the different lymphocyte subpopulations.", "contents": "Influence of different cytotoxic drugs on T and B lymphocytes in children with all during remission maintenance therapy. The influence of four different cytotoxic drugs (MTX, CYC, 6MP, and ARA-C) on T, B, and O-lymphocytes was investigated in 20 children with ALL in complete remission during cyclic remission maintenance therapy. Each of the four drugs causes a marked reduction of the absolute number of T and B cells whereas the relative number lies within the normal range with the exception of CYC, which leads to a depression of the percentage of both T and B cells. The percentage of O cells is markedly increased by CYC and slightly increased by MTX, 6MP, and ARA-C. The data are interpreted with care since the function of the immune system and especially tumor rejection depends on the interaction between the different lymphocyte subpopulations."}
{"id": "PMID:1087233", "title": "\"Early T cells\" and \"late T cells\"; suggestive evidence for two T cell lineages with separate developmental pathways.", "content": "Peripheral lymphocytes from mice have been analyzed by a combination of cell electrophoresis and size distribution analysis and the data have been processed with a computer into two-dimensional distribution patterns (fingerprints). The fingerprints of lymph node cells revealed the existence of at least three major classes of small lymphocytes. Cells with similar physical properties were found in the spleen and thoracic duct lymph. The data also allowed calculation of the quantitative ratios of the three cell types. In the normal mouse, the two electrophoretically faster cell classes represent essentially two subclasses of T cells. The quantitative ratios are here always in agreement with the known percentages of B and T cells. Lethally irradiated mice that have been reconstituted with syngeneic bone marrow or fetal liver cells often lack one of the T cell subclasses. This has two important implications. 1) It indicates that the widely used syngeneic or allogeneic bone marrow chimeras may be incomplete in respect to their T cell repertoire and that experiments with these models should be interpreted with caution. 2) The deficiency was found to correlate strictly with a deficiency in a physically defined subset of small cortical thymocytes that has been described previously. This correlation suggests strongly that the fast peripheral T cells and the \"early small cortical thymocytes\" represent two developmental stages of a distinct cell lineage (\"early T cell lineage\"), while the thymic pool of \"late small cortical thymocytes\" gives rise to electrophoretically slow peripheral T cells, which may be called \"late T cells). The possibility that both T cell lineages are derived from different classes of prethymic stem cells in the reconstitlting stem cell preparation is discussed. The proportion of \"early T cells\" in the lymph nodes decreases with age. The ratio of \"late\" and \"early T cells\" is similar in the spleen and in the lymph nodes, and both cell classes contain a significant proportion of cells that are not affected by the early effects of adult thymectomy. Thus, they do not correspond to T1 and T2 cells.", "contents": "\"Early T cells\" and \"late T cells\"; suggestive evidence for two T cell lineages with separate developmental pathways. Peripheral lymphocytes from mice have been analyzed by a combination of cell electrophoresis and size distribution analysis and the data have been processed with a computer into two-dimensional distribution patterns (fingerprints). The fingerprints of lymph node cells revealed the existence of at least three major classes of small lymphocytes. Cells with similar physical properties were found in the spleen and thoracic duct lymph. The data also allowed calculation of the quantitative ratios of the three cell types. In the normal mouse, the two electrophoretically faster cell classes represent essentially two subclasses of T cells. The quantitative ratios are here always in agreement with the known percentages of B and T cells. Lethally irradiated mice that have been reconstituted with syngeneic bone marrow or fetal liver cells often lack one of the T cell subclasses. This has two important implications. 1) It indicates that the widely used syngeneic or allogeneic bone marrow chimeras may be incomplete in respect to their T cell repertoire and that experiments with these models should be interpreted with caution. 2) The deficiency was found to correlate strictly with a deficiency in a physically defined subset of small cortical thymocytes that has been described previously. This correlation suggests strongly that the fast peripheral T cells and the \"early small cortical thymocytes\" represent two developmental stages of a distinct cell lineage (\"early T cell lineage\"), while the thymic pool of \"late small cortical thymocytes\" gives rise to electrophoretically slow peripheral T cells, which may be called \"late T cells). The possibility that both T cell lineages are derived from different classes of prethymic stem cells in the reconstitlting stem cell preparation is discussed. The proportion of \"early T cells\" in the lymph nodes decreases with age. The ratio of \"late\" and \"early T cells\" is similar in the spleen and in the lymph nodes, and both cell classes contain a significant proportion of cells that are not affected by the early effects of adult thymectomy. Thus, they do not correspond to T1 and T2 cells."}
{"id": "PMID:1087234", "title": "Immune conservation: a possible consequence of the mechanism of interferon-induced antibody suppression.", "content": "The effect of interferon preparations on the development of single clones of antibody-forming cells generated against sheep red blood cells in mouse spleen cell cultures has been studied. It was found that the presence of interferon had no effect on either the number or the size of clones which developed early in the in vitro antibody response. At the peak of the response (day 3), a decrease in the number but not in the size of clones was observed in interferon-treated compared to control cultures, while by day 4 both the size and number of clones were significantly decreased. These findings indicate that interferon appears to affect only nonactivated B cell precursors by preventing them from becoming activated and commencing clonal expansion. Early responding precursors, which are in cell cycle, presumably having been previously activated in vivo, are refractory to interferon-induced suppression in vitro. The way in which this immunosuppressive activity of interferon might be of great advantage to an animal as a means of \"immune conservation\" during viral infections is discussed.", "contents": "Immune conservation: a possible consequence of the mechanism of interferon-induced antibody suppression. The effect of interferon preparations on the development of single clones of antibody-forming cells generated against sheep red blood cells in mouse spleen cell cultures has been studied. It was found that the presence of interferon had no effect on either the number or the size of clones which developed early in the in vitro antibody response. At the peak of the response (day 3), a decrease in the number but not in the size of clones was observed in interferon-treated compared to control cultures, while by day 4 both the size and number of clones were significantly decreased. These findings indicate that interferon appears to affect only nonactivated B cell precursors by preventing them from becoming activated and commencing clonal expansion. Early responding precursors, which are in cell cycle, presumably having been previously activated in vivo, are refractory to interferon-induced suppression in vitro. The way in which this immunosuppressive activity of interferon might be of great advantage to an animal as a means of \"immune conservation\" during viral infections is discussed."}
{"id": "PMID:1087235", "title": "Surface molecules of cultured human lymphoid cells.", "content": "Cell surface molecules of cultured human lymphoid cells were selectively labeled by lactoperoxidase-catalyzed iodination and examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Two major iodinated species with apparent mol. wts. of 27 000 and 35 000 daltons were detected on autoradiographs of the labeled proteins of human lymphoid cell lines believed to be of thymus-independent (B) cell origin. Neither molecule was detected on putative thymus-dependent (T) lymphoid cell lines. Metabolic labeling studies showed that both molecules are glycoproteins. Rabbit antisera against cultured B lymphoid cells made specific by absorption for B cells reacted with several labeled species from iodinated B cells including the 27 000 and 35 000 mol. wt. glycoproteins. These molecules were also detected on tonsillar lymphocytes but not on peripheral blood lymphocytes. Reciprocal absorption with B cells of rabbit antisera against cultured T cells gave antisera specifically cytotoxic for T cells. However, these sera did not precipitate iodinated proteins from Nonidet P-40 lysates of T cells.", "contents": "Surface molecules of cultured human lymphoid cells. Cell surface molecules of cultured human lymphoid cells were selectively labeled by lactoperoxidase-catalyzed iodination and examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Two major iodinated species with apparent mol. wts. of 27 000 and 35 000 daltons were detected on autoradiographs of the labeled proteins of human lymphoid cell lines believed to be of thymus-independent (B) cell origin. Neither molecule was detected on putative thymus-dependent (T) lymphoid cell lines. Metabolic labeling studies showed that both molecules are glycoproteins. Rabbit antisera against cultured B lymphoid cells made specific by absorption for B cells reacted with several labeled species from iodinated B cells including the 27 000 and 35 000 mol. wt. glycoproteins. These molecules were also detected on tonsillar lymphocytes but not on peripheral blood lymphocytes. Reciprocal absorption with B cells of rabbit antisera against cultured T cells gave antisera specifically cytotoxic for T cells. However, these sera did not precipitate iodinated proteins from Nonidet P-40 lysates of T cells."}
{"id": "PMID:1087236", "title": "Immune response of New Zealand mice to trinitrophenylated syngeneic mouse red cells.", "content": "NZB and NZB/W mice have reduced anti-sheep red cell (SRC) and 2,4,6-trinitrophenyl-plaque-froming cell (TNP-PFC) responses with age after injection of either the thymus-dependent antigen TNP-SRC or the thymus-independent antigen TNP-mouse red cells (MRC). However, the thymus-dependent response diminished much faster than the thymus-independent response. As a consequence, young New Zealand mice have a higher anti-TNP response after injection of TNP-SRC than after injection of TNP-MRC, while old New Zealand mice have a higher anti-TNP response after injection of TNP-MRC than after injection of TNP-SRC. The PFC avidity of NZB/W mice injected with TNP-SRC diminished with age, while the PFC avidity of mice injected with TNP-MRC did not change with agrc or TNP-SRC. Old NZB/W mice had few spontaneous anti-MRC-PFC. The number of anti-MRC PFC in old mice was increased 4 to 10 times after injection with either TNP-SRC or TNP-MRC. It is suggested that surveillance mechanisms are responsible for suppressing the autoimmune response to modified self-antigens. The unregulated immune system of NZB and NZB/W mice appears to be an expression of impairment of such a hypothetical surveillance mechanism.", "contents": "Immune response of New Zealand mice to trinitrophenylated syngeneic mouse red cells. NZB and NZB/W mice have reduced anti-sheep red cell (SRC) and 2,4,6-trinitrophenyl-plaque-froming cell (TNP-PFC) responses with age after injection of either the thymus-dependent antigen TNP-SRC or the thymus-independent antigen TNP-mouse red cells (MRC). However, the thymus-dependent response diminished much faster than the thymus-independent response. As a consequence, young New Zealand mice have a higher anti-TNP response after injection of TNP-SRC than after injection of TNP-MRC, while old New Zealand mice have a higher anti-TNP response after injection of TNP-MRC than after injection of TNP-SRC. The PFC avidity of NZB/W mice injected with TNP-SRC diminished with age, while the PFC avidity of mice injected with TNP-MRC did not change with agrc or TNP-SRC. Old NZB/W mice had few spontaneous anti-MRC-PFC. The number of anti-MRC PFC in old mice was increased 4 to 10 times after injection with either TNP-SRC or TNP-MRC. It is suggested that surveillance mechanisms are responsible for suppressing the autoimmune response to modified self-antigens. The unregulated immune system of NZB and NZB/W mice appears to be an expression of impairment of such a hypothetical surveillance mechanism."}
{"id": "PMID:1087237", "title": "In vivo sensitization of T cells to hapten-conjugated syngeneic structures of major histocompatibility complex. I. Effect of in vitro culture upon generation of cytotoxic T lymphocytes.", "content": "Murine T cells were sensitized in vitro towards fluorescein isothiocyanate (FITC)-conjugated syngeneic spleen cells. The lytic activity of the effector cells generated was restricted to FITC-conjugated, H-2-compatible target cells. 2,4,6-Trinitrophenyl (TNP)-conjugated syngeneic targets were not lysed. A method is described for the in vivo sensitization of murine T cells towards TNP (or FITC)-conjugated syngeneic spleen cells. The procedure is based on the observation that a local graft of hapten-conjugated syngeneic spleen cells results in the development of sensitized prekiller T cells within the draining lymph node (LN). By mere in vitro incubation of such LN cells immunological specific anti-TNP (or anti-FITC)-reactive cytotoxic T lymphocytes (CTL) are generated. The in vitro differentiation of CTL requires 48-72 h; it is dependent on cell proliferation and is abolished by treatment of the LN cells with mitomycin C. Thus there is a separation between the sensitization of CTL precursors (which effectively takes place in vivo) and the phase of cell differentiation into CTL (which effectively takes place only in vitro). Similar to in vitro-sensitized CTL, the specificity of in vivo-sensitized CTL is dictated by the hapten used for the induction of the immune response and is restricted to hapten-conjugated syngeneic target cells.", "contents": "In vivo sensitization of T cells to hapten-conjugated syngeneic structures of major histocompatibility complex. I. Effect of in vitro culture upon generation of cytotoxic T lymphocytes. Murine T cells were sensitized in vitro towards fluorescein isothiocyanate (FITC)-conjugated syngeneic spleen cells. The lytic activity of the effector cells generated was restricted to FITC-conjugated, H-2-compatible target cells. 2,4,6-Trinitrophenyl (TNP)-conjugated syngeneic targets were not lysed. A method is described for the in vivo sensitization of murine T cells towards TNP (or FITC)-conjugated syngeneic spleen cells. The procedure is based on the observation that a local graft of hapten-conjugated syngeneic spleen cells results in the development of sensitized prekiller T cells within the draining lymph node (LN). By mere in vitro incubation of such LN cells immunological specific anti-TNP (or anti-FITC)-reactive cytotoxic T lymphocytes (CTL) are generated. The in vitro differentiation of CTL requires 48-72 h; it is dependent on cell proliferation and is abolished by treatment of the LN cells with mitomycin C. Thus there is a separation between the sensitization of CTL precursors (which effectively takes place in vivo) and the phase of cell differentiation into CTL (which effectively takes place only in vitro). Similar to in vitro-sensitized CTL, the specificity of in vivo-sensitized CTL is dictated by the hapten used for the induction of the immune response and is restricted to hapten-conjugated syngeneic target cells."}
{"id": "PMID:1087239", "title": "Lack of effect of thymectomy on spontaneous recovery from tolerance to levan.", "content": "Adult CBA mice were made tolerant with 1 mg levan and tested subsequently for sponteneous recovery by plaque-forming cell assay in response to immunizing challenge. Weakening of tolerance was first detectable only after 100 days, while return to full normal responsiveness required 400 days. Thymectomy 2 weeks prior to tolerization neither retarded nor diminished the recovery process. Loss of tolerance which had been induced by 1 mg dextran B512 was much slower in onset (greater than 250 days), yet not delayed by thymectomy (full recovery was not followed). These findings in relation to other considerations imply that associative recognition by T cells is not required for B cell responses to these \"thymus-independent\" antigens.", "contents": "Lack of effect of thymectomy on spontaneous recovery from tolerance to levan. Adult CBA mice were made tolerant with 1 mg levan and tested subsequently for sponteneous recovery by plaque-forming cell assay in response to immunizing challenge. Weakening of tolerance was first detectable only after 100 days, while return to full normal responsiveness required 400 days. Thymectomy 2 weeks prior to tolerization neither retarded nor diminished the recovery process. Loss of tolerance which had been induced by 1 mg dextran B512 was much slower in onset (greater than 250 days), yet not delayed by thymectomy (full recovery was not followed). These findings in relation to other considerations imply that associative recognition by T cells is not required for B cell responses to these \"thymus-independent\" antigens."}
{"id": "PMID:1087240", "title": "Functional half-life of virgin and primed B lymphocytes.", "content": "The functional half-life, a measure of the persistence of lymphocytes in an antigen-free environment, has been estimated and found to be about 7 days for virgin 2,4,6-trinitrophenyl (TNP)-reactive mouse B cells, and 2--3 times longer for TNP-primed B cells. Using allotype-congenic mice, lymph node cells were transferred from virgin or primed CBA/Igb donors to normal CBA/Iga recipients, and the proportion of donor B cells estimated at intervals...", "contents": "Functional half-life of virgin and primed B lymphocytes. The functional half-life, a measure of the persistence of lymphocytes in an antigen-free environment, has been estimated and found to be about 7 days for virgin 2,4,6-trinitrophenyl (TNP)-reactive mouse B cells, and 2--3 times longer for TNP-primed B cells. Using allotype-congenic mice, lymph node cells were transferred from virgin or primed CBA/Igb donors to normal CBA/Iga recipients, and the proportion of donor B cells estimated at intervals..."}
{"id": "PMID:1087241", "title": "Structural aspects of immune recognition of lysozymes. III. T cell specificity restriction and its consequences for antibody specificity.", "content": "We have studied the relationship between the determinants encountered by T cells on an antigenic molecule and the specificities of the antibodies eventually produced by the B cells with which these T cells cooperate. The number of  epitopes on the hen lysozyme (HEL) molecule available to T cell receptors was functionally limited by inducing T cell tolerance to HEL in rabbits. Highly cross-reactive lysozymes were then used to challenge the HEL-unresponsive rabbits. Only T cells which recognize new epitopes on the challenge lysozymes could act as helpers in generating an anti-lysozyme response. Amino acid differences between Japanese quail lysozyme (JEL) and HEL are segregated within a single quadrant of this small antigen molecule. HEL-tolerant rabbits challenged with JEL produced antibodies which were totally cross-reactive with the tolerogen HEL. This result is in contrast to the result obtained in nontolerant rabbits which produced antibodies to JEL which were only 50-70% cross-reactive with HEL. We conclude that T cells restricted to the JEL-unique epitopes were only capable of cooperating with B cells specific for common epitopes shared between JEL and the tolerogen HEL. Turkey lysozyme (TEL), on the other hand, bears different amino acids which are distributed over several regions on the surface of the molecule. Any one HEL-tolerant rabbit developed a restricted response to TEL; in some rabbits the anti-TEL was highly HEL cross-reactive, while in others little cross-reactivity with HEL was observed. Each of four HEL-tolerant rabbits injected with the minimally altered bob-white quail lysozyme possessed the reactive T cells necessary to mount a limited response to this challenge lysozyme, suggesting a diverse library of T cell specificities. Recognition of the small differences between the challenge lysozymes and the T cells of these tolerant rabbits to make a fine discrimination between minimally changed epitopes.", "contents": "Structural aspects of immune recognition of lysozymes. III. T cell specificity restriction and its consequences for antibody specificity. We have studied the relationship between the determinants encountered by T cells on an antigenic molecule and the specificities of the antibodies eventually produced by the B cells with which these T cells cooperate. The number of  epitopes on the hen lysozyme (HEL) molecule available to T cell receptors was functionally limited by inducing T cell tolerance to HEL in rabbits. Highly cross-reactive lysozymes were then used to challenge the HEL-unresponsive rabbits. Only T cells which recognize new epitopes on the challenge lysozymes could act as helpers in generating an anti-lysozyme response. Amino acid differences between Japanese quail lysozyme (JEL) and HEL are segregated within a single quadrant of this small antigen molecule. HEL-tolerant rabbits challenged with JEL produced antibodies which were totally cross-reactive with the tolerogen HEL. This result is in contrast to the result obtained in nontolerant rabbits which produced antibodies to JEL which were only 50-70% cross-reactive with HEL. We conclude that T cells restricted to the JEL-unique epitopes were only capable of cooperating with B cells specific for common epitopes shared between JEL and the tolerogen HEL. Turkey lysozyme (TEL), on the other hand, bears different amino acids which are distributed over several regions on the surface of the molecule. Any one HEL-tolerant rabbit developed a restricted response to TEL; in some rabbits the anti-TEL was highly HEL cross-reactive, while in others little cross-reactivity with HEL was observed. Each of four HEL-tolerant rabbits injected with the minimally altered bob-white quail lysozyme possessed the reactive T cells necessary to mount a limited response to this challenge lysozyme, suggesting a diverse library of T cell specificities. Recognition of the small differences between the challenge lysozymes and the T cells of these tolerant rabbits to make a fine discrimination between minimally changed epitopes."}
{"id": "PMID:1087242", "title": "T cell requirements for the expression of the lipopolysaccharide adjuvant effect in vivo: evidence for a T cell-dependent and a T cell-independent mode of action.", "content": "The in vivo adjuvant effect of lipopolysaccharide (LPS) in mice was investigated with the soluble synthetic polypeptide antigen (T, G)-A--L, the antibody response to which is determined by the Ir-1A gene. With this specific antigen it can be demonstrated that the LPS adjuvant effect has the following modes of action: a) a T cell-dependent enhancement of primary and secondary IgM antibody response; b) a T cell-dependent enhancement of IgG secondary andibody response; and c) a T cell-dependent induction of switchover from IgM to IgG andibody in some strains of Ir-1A low responders. Although T cells are necessary for some aspects of the adjuvant effect, these data do not distinguish between a mechanism involving a direct interaction between LPS and T cells or a direct interaction of LPS and B cells with a general requirement for T cells for expression of IgG antibody.", "contents": "T cell requirements for the expression of the lipopolysaccharide adjuvant effect in vivo: evidence for a T cell-dependent and a T cell-independent mode of action. The in vivo adjuvant effect of lipopolysaccharide (LPS) in mice was investigated with the soluble synthetic polypeptide antigen (T, G)-A--L, the antibody response to which is determined by the Ir-1A gene. With this specific antigen it can be demonstrated that the LPS adjuvant effect has the following modes of action: a) a T cell-dependent enhancement of primary and secondary IgM antibody response; b) a T cell-dependent enhancement of IgG secondary andibody response; and c) a T cell-dependent induction of switchover from IgM to IgG andibody in some strains of Ir-1A low responders. Although T cells are necessary for some aspects of the adjuvant effect, these data do not distinguish between a mechanism involving a direct interaction between LPS and T cells or a direct interaction of LPS and B cells with a general requirement for T cells for expression of IgG antibody."}
{"id": "PMID:1087243", "title": "T cell necessity in the pathogenesis of experimental autoimmune encephalomyelitis in mice.", "content": "Cellular transfer of experimental autoimmune encephalomyelitis (EAE) was effected in mice with lymph node and spleen cells from appropriately immunized donors. In contrast to lymphoid cells, immune serum did not transfer this autoimmune disease nor did serum have any facilitating or inhibitory effect on the capacity of lymphoid cells to transfer EAE. Transfer of EAE was effected in normal mice, lightly irradiated (350 rad) and lethally irradiated (850 rad) and bone marrow-protected mice, but not in mice which had been given 850 rad total-body irradiation. There was a striking augmentation of severity of transferred EAE in the lightly irradiated recipients, possibly attributable to selective radiosensitivity of suppressor T cells. Cell-mediated immunity but not circulating antibody to basic protein of myelin was demonstrated in recipients with transferred EAE. The immune lymphoid cells responsible for transfer of EAE were T lymphocytes. Thus transfer was successful after passage of sensitized cells through anti-immunoglobulin columns and was abrogated following treatment with anti-Thy-1 serum and complement. Neonatally thymectomized mice failed to develop either EAE, cell mediated immunity or humoral antibody against myelin basic protein (BPM). Inhibition of EAE and immune responsiveness was solely due to the removal of the source of thymus lymphocytes, because reconstitution of neonatally thymectomized mice with T lymphocytes completely restored these functions. It is concluded that T lymphocytes are required for the production and adoptive transfer of EAE, for the development of cell-mediated immunity to BPM and for the production of antibody to BPM.", "contents": "T cell necessity in the pathogenesis of experimental autoimmune encephalomyelitis in mice. Cellular transfer of experimental autoimmune encephalomyelitis (EAE) was effected in mice with lymph node and spleen cells from appropriately immunized donors. In contrast to lymphoid cells, immune serum did not transfer this autoimmune disease nor did serum have any facilitating or inhibitory effect on the capacity of lymphoid cells to transfer EAE. Transfer of EAE was effected in normal mice, lightly irradiated (350 rad) and lethally irradiated (850 rad) and bone marrow-protected mice, but not in mice which had been given 850 rad total-body irradiation. There was a striking augmentation of severity of transferred EAE in the lightly irradiated recipients, possibly attributable to selective radiosensitivity of suppressor T cells. Cell-mediated immunity but not circulating antibody to basic protein of myelin was demonstrated in recipients with transferred EAE. The immune lymphoid cells responsible for transfer of EAE were T lymphocytes. Thus transfer was successful after passage of sensitized cells through anti-immunoglobulin columns and was abrogated following treatment with anti-Thy-1 serum and complement. Neonatally thymectomized mice failed to develop either EAE, cell mediated immunity or humoral antibody against myelin basic protein (BPM). Inhibition of EAE and immune responsiveness was solely due to the removal of the source of thymus lymphocytes, because reconstitution of neonatally thymectomized mice with T lymphocytes completely restored these functions. It is concluded that T lymphocytes are required for the production and adoptive transfer of EAE, for the development of cell-mediated immunity to BPM and for the production of antibody to BPM."}
{"id": "PMID:1087244", "title": "T lineage lymphocytes in nude mice born from homozygous nu/nu parents.", "content": "T lineage cells are found in the spleen of neonatal nu/nu mice born from nu/nu x nu/nu matings, and also in old, healthy nude mice obtained from the usual nu/+ x nu/nu matings. Their presence in neonatal nude mice which were never in contact with mother or normal littermate thymus products shows that commitment of stem cells towards the T lineage does not require the influence of the thymus.", "contents": "T lineage lymphocytes in nude mice born from homozygous nu/nu parents. T lineage cells are found in the spleen of neonatal nu/nu mice born from nu/nu x nu/nu matings, and also in old, healthy nude mice obtained from the usual nu/+ x nu/nu matings. Their presence in neonatal nude mice which were never in contact with mother or normal littermate thymus products shows that commitment of stem cells towards the T lineage does not require the influence of the thymus."}
{"id": "PMID:1087254", "title": "Transplantation tolerance at the T-lymphocyte receptor level. I. Spontaneous release of T-cell receptors by lymphocytes from tolerant mice.", "content": "Spleen and lymph node T cells from CBA mice when cultivated in vitro almost immediately started to shed receptors for A alloantigens. In contrast, cultivated T lymphocytes from CBA mice tolerant of A alloantigens for 657 or 692 days began releasing receptors for the formerly tolerated A alloantigen after a delay of 8 h. This delay in receptor shedding coincided with that observed when nontolerant lymphocytes were treated with antisera to T-cell receptors. The results suggested a close similarity of mechanisms and indicated that transplantation tolerance may be maintained by active suppression of otherwise reactive T cells mediated by anti-receptor antibody. Lymphocytes from untreated tolerant mice and those treated with a nonspecific anti-receptor serum showed normal responses, as measured in terms of receptor release, to third-party alloantigens, as did cells from untreated normal mice. Precultivated normal lymphocytes treated with anti-receptor antibody and complement failed to release the appropriate receptor specificity but similarly treated tolerant lymphocytes were almost resistant, presumably because they expressed only few receptors at the time of treatments.", "contents": "Transplantation tolerance at the T-lymphocyte receptor level. I. Spontaneous release of T-cell receptors by lymphocytes from tolerant mice. Spleen and lymph node T cells from CBA mice when cultivated in vitro almost immediately started to shed receptors for A alloantigens. In contrast, cultivated T lymphocytes from CBA mice tolerant of A alloantigens for 657 or 692 days began releasing receptors for the formerly tolerated A alloantigen after a delay of 8 h. This delay in receptor shedding coincided with that observed when nontolerant lymphocytes were treated with antisera to T-cell receptors. The results suggested a close similarity of mechanisms and indicated that transplantation tolerance may be maintained by active suppression of otherwise reactive T cells mediated by anti-receptor antibody. Lymphocytes from untreated tolerant mice and those treated with a nonspecific anti-receptor serum showed normal responses, as measured in terms of receptor release, to third-party alloantigens, as did cells from untreated normal mice. Precultivated normal lymphocytes treated with anti-receptor antibody and complement failed to release the appropriate receptor specificity but similarly treated tolerant lymphocytes were almost resistant, presumably because they expressed only few receptors at the time of treatments."}
{"id": "PMID:1087255", "title": "Transplantation tolerance at the T-lymphocyte receptor level. II. Interaction of T-cell receptors with alloantigen and with anti-receptor antiserum.", "content": "CBA T lymphocytes deprived temporarily of receptors for alloantigens A[RS(A)] cultivated in vitro for 30 h with anti-receptor antibody-forming (AxCBA)F1 spleen cells were capable of resynthesizing RS(A) if primed F1 cells exceeded parental T cells by a factor of 25 or less, but not if the excess was 50-fold or more. This indicated that resynthesis of CBA T-cell RS(A) was successful if primed F1 cells formed insufficient amounts of anti-CBA T-cell RS(A) antibody. Abortive or successful receptor resynthesis was measured by two parameters, (a) reappearing RS(A) formed PAR together with A alloantigens of (AxCBA)F1 spleen cells and (b) budding receptors bound anti-receptor antibody. CBA B lymphocutes did not interfere with these reactions. A search for putative T suppressor cells in the F1 cell population was unsuccessful. PAR formation and anti--RS antibody consumption by reappearing receptors differed temporally: receptors forming PAR were present after a delay lasting 8 h; receptor structures fixed anti-RS antibody as early as 5 h after being cultivated. With due caution, these results might reflect processes operating in maintenance of transplantation tolerance, suggesting that this condition is a serum-mediated suppression of long duration. The suppression would encompass continued neutralisation of receptors for the alloantigen to be tolerated by anti-T-cell receptor antibody formed by the F1 chimeric cells within an animal with acquired transplantation tolerance.", "contents": "Transplantation tolerance at the T-lymphocyte receptor level. II. Interaction of T-cell receptors with alloantigen and with anti-receptor antiserum. CBA T lymphocytes deprived temporarily of receptors for alloantigens A[RS(A)] cultivated in vitro for 30 h with anti-receptor antibody-forming (AxCBA)F1 spleen cells were capable of resynthesizing RS(A) if primed F1 cells exceeded parental T cells by a factor of 25 or less, but not if the excess was 50-fold or more. This indicated that resynthesis of CBA T-cell RS(A) was successful if primed F1 cells formed insufficient amounts of anti-CBA T-cell RS(A) antibody. Abortive or successful receptor resynthesis was measured by two parameters, (a) reappearing RS(A) formed PAR together with A alloantigens of (AxCBA)F1 spleen cells and (b) budding receptors bound anti-receptor antibody. CBA B lymphocutes did not interfere with these reactions. A search for putative T suppressor cells in the F1 cell population was unsuccessful. PAR formation and anti--RS antibody consumption by reappearing receptors differed temporally: receptors forming PAR were present after a delay lasting 8 h; receptor structures fixed anti-RS antibody as early as 5 h after being cultivated. With due caution, these results might reflect processes operating in maintenance of transplantation tolerance, suggesting that this condition is a serum-mediated suppression of long duration. The suppression would encompass continued neutralisation of receptors for the alloantigen to be tolerated by anti-T-cell receptor antibody formed by the F1 chimeric cells within an animal with acquired transplantation tolerance."}
{"id": "PMID:1087260", "title": "Meiotic origin of triploidy in the frog detected by genetic analysis of enzyme polymorphisms.", "content": "A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chromosome counts of tail tip cells. Most of these triploids had both maternal alleles at the MDH and MPI loci suggesting that the first meiotic division was repressed. Others seemed to represent a repressed second meiotic division and one animal, a pentaploid, could only have resulted from inhibition of both meiotic divisions of the egg. Densitometer tracings of starch gels stained for 6 phosphogluconate and isocitrate dehydrogenases, expected to be heterozygous in a particular cross, demonstrated that the triploids had twice as much maternal as paternal gene product for each locus, similar to patterns found in triploids produced by nuclear transplantation.", "contents": "Meiotic origin of triploidy in the frog detected by genetic analysis of enzyme polymorphisms. A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chromosome counts of tail tip cells. Most of these triploids had both maternal alleles at the MDH and MPI loci suggesting that the first meiotic division was repressed. Others seemed to represent a repressed second meiotic division and one animal, a pentaploid, could only have resulted from inhibition of both meiotic divisions of the egg. Densitometer tracings of starch gels stained for 6 phosphogluconate and isocitrate dehydrogenases, expected to be heterozygous in a particular cross, demonstrated that the triploids had twice as much maternal as paternal gene product for each locus, similar to patterns found in triploids produced by nuclear transplantation."}
{"id": "PMID:1087261", "title": "Anemia--a common but never a normal concomitant of aging.", "content": "Diagnosis is often overlooked because symptoms develop slowly and insidiously and many patients don't complain about them. Then too, the giddiness, apathy, confusion, clumsiness, and similar problems may be considered simply signs of \"old age.\" Iron deficiency anemia is the most common type in old people. It's usually due to gastrointestinal bleeding, but there may be a second, less obvious cause. The classic picture of low serum iron, high total iron-binding capacity, and low iron-binding saturation is sometimes distorted. Usually, many studies are needed to confirm the suspicion of a vitamin B12 or folic acid deficiency. A raised mean corpuscular volume in itself signals the need for further investigation. In patients with macrocytosis, the bone marrow must be examined. Tests for intestinal malabsorption must be considered too. Repeated blood tests are essential in patients being treated for any type of anemia. Iron deficiency may hide evidence of folate or B12 deficiency. And iron therapy may lessen bleeding from colonic cancer, delaying diagnosis until it's too late to operate.", "contents": "Anemia--a common but never a normal concomitant of aging. Diagnosis is often overlooked because symptoms develop slowly and insidiously and many patients don't complain about them. Then too, the giddiness, apathy, confusion, clumsiness, and similar problems may be considered simply signs of \"old age.\" Iron deficiency anemia is the most common type in old people. It's usually due to gastrointestinal bleeding, but there may be a second, less obvious cause. The classic picture of low serum iron, high total iron-binding capacity, and low iron-binding saturation is sometimes distorted. Usually, many studies are needed to confirm the suspicion of a vitamin B12 or folic acid deficiency. A raised mean corpuscular volume in itself signals the need for further investigation. In patients with macrocytosis, the bone marrow must be examined. Tests for intestinal malabsorption must be considered too. Repeated blood tests are essential in patients being treated for any type of anemia. Iron deficiency may hide evidence of folate or B12 deficiency. And iron therapy may lessen bleeding from colonic cancer, delaying diagnosis until it's too late to operate."}
{"id": "PMID:1087262", "title": "Depressed cell-mediated immunity in coeliac disease.", "content": "Fourteen coeliac patients on a gluten free diet (GFD) and 10 on a normal diet were studied by lymphocyte transformation in response to PHA to assess the integrity of cell-mediated immunity (CMI). Transformation was depressed in the majority taking a normal diet, with improvement after a GFD. In some patients the depression may have been due to a serum factor, as transformation was more nearly normal when the lymphocytes were cultured in pooled AB serum than in their own serum. There was no correlation between transformation and nutritional deficiencies. Mantoux tests were performed in some of these and other coeliac patients and there was a very significant reduction in the incidence of positive tests compared with controls. These findings provide evidence of depressed CMI in coeliac patients taking a normal diet with improvement on a GFD and may be of relevance to the high risk of malignancy in coeliac disease, further strengthening the case for a strict GFD.", "contents": "Depressed cell-mediated immunity in coeliac disease. Fourteen coeliac patients on a gluten free diet (GFD) and 10 on a normal diet were studied by lymphocyte transformation in response to PHA to assess the integrity of cell-mediated immunity (CMI). Transformation was depressed in the majority taking a normal diet, with improvement after a GFD. In some patients the depression may have been due to a serum factor, as transformation was more nearly normal when the lymphocytes were cultured in pooled AB serum than in their own serum. There was no correlation between transformation and nutritional deficiencies. Mantoux tests were performed in some of these and other coeliac patients and there was a very significant reduction in the incidence of positive tests compared with controls. These findings provide evidence of depressed CMI in coeliac patients taking a normal diet with improvement on a GFD and may be of relevance to the high risk of malignancy in coeliac disease, further strengthening the case for a strict GFD."}
{"id": "PMID:1087263", "title": "Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis.", "content": "Skin reactivity to dinitrochlorobenzene (DNCB) and levels of circulating T-lymphocytes were measured in 15 patients with ulcerative colitis, 15 patients with Crohn's disease, and 12 normal control subjects. Diminished reactivity to DNCB was demonstrated in 87% of patients with Crohn's disease (P less than 0-001) and in 53% with ulcerative colitis (P less than 0-02), as compared with only 8-5% of controls; anergy was more frequent in Crohn's disease than in ulcerative colitis (P less than 0-05). Levels of circulating T-lymphoctes were also depressed in both Crohn's disease and ulcerative colitis (P less than 0-001) as compared with controls, with the values lower in Crohn's disease than in ulcerative colitis (P less than 0-02). There were no correlations of DNCB response with extent, duration, or severity of disease nor with T-cell levels within any patient group. These data provide further support for the concept that there is impairment of cell-mediated immunity among many patients with chronic inflammatory bowel disease, including both Crohn's disease and ulcerative colitis.", "contents": "Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis. Skin reactivity to dinitrochlorobenzene (DNCB) and levels of circulating T-lymphocytes were measured in 15 patients with ulcerative colitis, 15 patients with Crohn's disease, and 12 normal control subjects. Diminished reactivity to DNCB was demonstrated in 87% of patients with Crohn's disease (P less than 0-001) and in 53% with ulcerative colitis (P less than 0-02), as compared with only 8-5% of controls; anergy was more frequent in Crohn's disease than in ulcerative colitis (P less than 0-05). Levels of circulating T-lymphoctes were also depressed in both Crohn's disease and ulcerative colitis (P less than 0-001) as compared with controls, with the values lower in Crohn's disease than in ulcerative colitis (P less than 0-02). There were no correlations of DNCB response with extent, duration, or severity of disease nor with T-cell levels within any patient group. These data provide further support for the concept that there is impairment of cell-mediated immunity among many patients with chronic inflammatory bowel disease, including both Crohn's disease and ulcerative colitis."}
{"id": "PMID:1087281", "title": "Effect of hydrocortisone on response of human lymphocytes to galactose oxidase stimulation.", "content": "Hydrocortisone at 10(-4)M concentration could inhibit the response of human lymphocytes to galactose oxidase stimulation. Removal of the hydrocortisone after 4 h of culture did not reverse this inhibition. On the other hand, removal of monocytes greatly enhanced it, so that it could be observed at 10(-6)M concentration. Next, tonsil cells were separated into two fractions relatively rich in either T or B cells. Both fractions could respond to galactose oxidase and the response could be inhibited by hydrocortisone.", "contents": "Effect of hydrocortisone on response of human lymphocytes to galactose oxidase stimulation. Hydrocortisone at 10(-4)M concentration could inhibit the response of human lymphocytes to galactose oxidase stimulation. Removal of the hydrocortisone after 4 h of culture did not reverse this inhibition. On the other hand, removal of monocytes greatly enhanced it, so that it could be observed at 10(-6)M concentration. Next, tonsil cells were separated into two fractions relatively rich in either T or B cells. Both fractions could respond to galactose oxidase and the response could be inhibited by hydrocortisone."}
{"id": "PMID:1087282", "title": "Influence of diftalone on tolbutamide test and i.v. glucose tolerance test.", "content": "In 16 healthy volunteers tolbutamide tests or i.v. glucose tolerance tests were performed with and without previous oral administration of 1000 mg diftalone. Blood sugar and serum insulin were assayed in regular intervals. Both with and without previous administration of diftalone blood glucose after tolbutamide did not show any difference. IRI response to tolbutamide, measured by planimetrical integration showed a statistically significant augmentation (0.05 greater than p greater than 0.01) after diftalone. Glucose assimilation (K-value) after diftalone was decreased (0.05 greater than p greater than 0.01) yet within normal range. For the accompanying insulin levels however no statistically significant difference was observed. In addition a normalisation of pathological tolbutamide test after diftalone could be noted in five patients with subclinical diabetes. Our results indicate that diftalone seems to have the following three actions: 1. Enhancement of the tolbutamide action. 2. direct augmentation of IRI secretion, 3. a peripheral action on glucose metabolism.", "contents": "Influence of diftalone on tolbutamide test and i.v. glucose tolerance test. In 16 healthy volunteers tolbutamide tests or i.v. glucose tolerance tests were performed with and without previous oral administration of 1000 mg diftalone. Blood sugar and serum insulin were assayed in regular intervals. Both with and without previous administration of diftalone blood glucose after tolbutamide did not show any difference. IRI response to tolbutamide, measured by planimetrical integration showed a statistically significant augmentation (0.05 greater than p greater than 0.01) after diftalone. Glucose assimilation (K-value) after diftalone was decreased (0.05 greater than p greater than 0.01) yet within normal range. For the accompanying insulin levels however no statistically significant difference was observed. In addition a normalisation of pathological tolbutamide test after diftalone could be noted in five patients with subclinical diabetes. Our results indicate that diftalone seems to have the following three actions: 1. Enhancement of the tolbutamide action. 2. direct augmentation of IRI secretion, 3. a peripheral action on glucose metabolism."}
{"id": "PMID:1087283", "title": "The effects of U.V.-light, ionizing radiation and the carcinogen N-acetoxy-2-fluorenylacetamide on the development in vitro of one- and two-cell mouse embryos.", "content": "The development of one- and two-cell mouse embryos to morula-blastula stages was followed in vitro after treatment with low doses of U.V.-light, ionizing radiation or N-acetoxy-2-fluorenylacetamide. Exposure of one-cell embryos to either radiation source 18 and 24 hours after human chorionic gonadotropin injections prevented maturation, most embryos being arrested at the one-cell stage and a few at the two-cell stage. Two-cell embryos, however, were not sensitive to low doses of either U.V. or X-irradiation and developed normally. Treatment of early one-cell embryos with the carcinogen, N-acetoxy-2-fluorenyl-acetamide (0-7 muM), also arrested development, whereas exposure of late one-cell embryos did not completely prevent maturation to morula-blastula stages. Exposure of two-cell embryos to the same concentration of carcinogen had no effect on their development to blastulas. Results with all three agents showed that mouse embryos at the one-cell stage are more sensitive than those at the two-cell stage, as judged by their ability to develop in vitro.", "contents": "The effects of U.V.-light, ionizing radiation and the carcinogen N-acetoxy-2-fluorenylacetamide on the development in vitro of one- and two-cell mouse embryos. The development of one- and two-cell mouse embryos to morula-blastula stages was followed in vitro after treatment with low doses of U.V.-light, ionizing radiation or N-acetoxy-2-fluorenylacetamide. Exposure of one-cell embryos to either radiation source 18 and 24 hours after human chorionic gonadotropin injections prevented maturation, most embryos being arrested at the one-cell stage and a few at the two-cell stage. Two-cell embryos, however, were not sensitive to low doses of either U.V. or X-irradiation and developed normally. Treatment of early one-cell embryos with the carcinogen, N-acetoxy-2-fluorenyl-acetamide (0-7 muM), also arrested development, whereas exposure of late one-cell embryos did not completely prevent maturation to morula-blastula stages. Exposure of two-cell embryos to the same concentration of carcinogen had no effect on their development to blastulas. Results with all three agents showed that mouse embryos at the one-cell stage are more sensitive than those at the two-cell stage, as judged by their ability to develop in vitro."}
{"id": "PMID:1087284", "title": "Recovery response of dividing cells in the thymus of whole-body gamma-irradiated mice.", "content": "Mice were irradiated with different doses of gamma-rays 30 min after the administration of 32P-orthophosphate. The dose-response curves determined at 72 hours after exposure showed an inflection point in the total activity present in the DNA in thymus and spleen. In the low dose-range, the dose-response curves have D0 = 55 rad (n = 2-5) for thymus and DO = 95 rad (n = 2-5) for the spleen. Thirty minutes after the administration of 32P-orthophosphate, the dividing cells from thymus were partially synchronized by the administration of 80 mg per kg body-weight hydroxyurea. At different time-intervals, the mice were irradiated with 80 rad, and the total activity of DNA was determined at 72 hours after synchronization. A significant maximum of recovery was found at 5 hours (S phase) after the administration of hydroxyurea. In similar conditions, the dose-response curves corresponding to the G1, S and M phase of the division cycle were also determined. The synchronization of dividing cells induced by hydroxyurea failed in the spleen.", "contents": "Recovery response of dividing cells in the thymus of whole-body gamma-irradiated mice. Mice were irradiated with different doses of gamma-rays 30 min after the administration of 32P-orthophosphate. The dose-response curves determined at 72 hours after exposure showed an inflection point in the total activity present in the DNA in thymus and spleen. In the low dose-range, the dose-response curves have D0 = 55 rad (n = 2-5) for thymus and DO = 95 rad (n = 2-5) for the spleen. Thirty minutes after the administration of 32P-orthophosphate, the dividing cells from thymus were partially synchronized by the administration of 80 mg per kg body-weight hydroxyurea. At different time-intervals, the mice were irradiated with 80 rad, and the total activity of DNA was determined at 72 hours after synchronization. A significant maximum of recovery was found at 5 hours (S phase) after the administration of hydroxyurea. In similar conditions, the dose-response curves corresponding to the G1, S and M phase of the division cycle were also determined. The synchronization of dividing cells induced by hydroxyurea failed in the spleen."}
{"id": "PMID:1087285", "title": "Investigation of the interaction of alkyl sulphates with serum albumin using the thiocyanate radical ion (SCN)2.", "content": "The reaction of the radical anion -(SCN)2-, produced during pulse radilysis of aqueous KCNS solutions, have been used to study the binding of a range of alkyl sulphates to bovine (BSA) and human (HSA) serum albumin. At neutral pH, -(SCN)2- reacts chiefly with trytophan residues. Approximately ten high-affinity binding sites are detectable for compounds of chain length greater than C7. The results are interpreted in terms of a model in which one hydrophobic region in the protein, containing the tryptophan residues, can accommodate the ten ligand molecules. Electrostatic interactions with positively-charged groups surrounding the hydrophobic area are also involved in binding.", "contents": "Investigation of the interaction of alkyl sulphates with serum albumin using the thiocyanate radical ion (SCN)2. The reaction of the radical anion -(SCN)2-, produced during pulse radilysis of aqueous KCNS solutions, have been used to study the binding of a range of alkyl sulphates to bovine (BSA) and human (HSA) serum albumin. At neutral pH, -(SCN)2- reacts chiefly with trytophan residues. Approximately ten high-affinity binding sites are detectable for compounds of chain length greater than C7. The results are interpreted in terms of a model in which one hydrophobic region in the protein, containing the tryptophan residues, can accommodate the ten ligand molecules. Electrostatic interactions with positively-charged groups surrounding the hydrophobic area are also involved in binding."}
{"id": "PMID:1087286", "title": "Survival of human T and B lymphocytes after X-irradiation.", "content": "The survival of unstimulated human T and B lymphocytes after X-irradiation in vitro was measured by Trypan Blue dye exclusion over a period of four days. B cell numbers were observed to decline rapidly even after relatively low doses, but T cell numbers fell much more slowly. A comparison of the percentage survival 96 hours after irradiation shows that in this system T cells are between approximately 2 and 5 times more resistant than B cells. Data for interphase death after 48 hours are compared with cytogenetic data for interphase loss of PHA-stimulated human lymphocytes and are shown to be in broad agreement at radiation doses below 400 rad. It is suggested that at higher doses mitotic delay may be increasingly important leading to selection of non-irradiated cells at 48 hours.", "contents": "Survival of human T and B lymphocytes after X-irradiation. The survival of unstimulated human T and B lymphocytes after X-irradiation in vitro was measured by Trypan Blue dye exclusion over a period of four days. B cell numbers were observed to decline rapidly even after relatively low doses, but T cell numbers fell much more slowly. A comparison of the percentage survival 96 hours after irradiation shows that in this system T cells are between approximately 2 and 5 times more resistant than B cells. Data for interphase death after 48 hours are compared with cytogenetic data for interphase loss of PHA-stimulated human lymphocytes and are shown to be in broad agreement at radiation doses below 400 rad. It is suggested that at higher doses mitotic delay may be increasingly important leading to selection of non-irradiated cells at 48 hours."}
{"id": "PMID:1087287", "title": "Reaction of ethanol radicals with ribonuclease.", "content": "Ribonuclease was irradiated under conditions such that ethanol radicals were the main reactive species in solution. Sephadex gel filtration of the irradiated solution demonstrated that ethanol radicals had reacted with the ribonuclease and had become firmly bound to the enzyme molecule. The number of ethanol molecules bound to ribonuclease was a function of dose and correlated with the loss of enzymatic activity and with the changes in the molecular configuration of the enzyme molecule.", "contents": "Reaction of ethanol radicals with ribonuclease. Ribonuclease was irradiated under conditions such that ethanol radicals were the main reactive species in solution. Sephadex gel filtration of the irradiated solution demonstrated that ethanol radicals had reacted with the ribonuclease and had become firmly bound to the enzyme molecule. The number of ethanol molecules bound to ribonuclease was a function of dose and correlated with the loss of enzymatic activity and with the changes in the molecular configuration of the enzyme molecule."}
{"id": "PMID:1087288", "title": "A comparison of physical and cytogenetic estimates of radiation dose in patients treated with iodine-131 for thyroid carcinoma.", "content": "Physical and cytogenetic estimates of the whole-body radiation doses have been compared in 11 patients receiving large doses of iodine-131 for the treatment of thyroid carcinoma. The physical estimate was based on the measurement of thyroid uptake, of the plasma activity variation, and of urinary activity. The cytogenetic estimate was obtained from the analysis of chromosome aberrations in peripheral blood lymphocytes. Good agreement between the estimates was observed in patients whose thyroid glands had previously been ablated by radioiodine. In patients who had varying degrees of thyroid function, there were considerable differences between the estimates with the cytogenetic value always being higher. It is suggested that these differences might be due in part to non-uniform irradiation of lymphocytes by local sources of activity in the thyroid and in the liver.", "contents": "A comparison of physical and cytogenetic estimates of radiation dose in patients treated with iodine-131 for thyroid carcinoma. Physical and cytogenetic estimates of the whole-body radiation doses have been compared in 11 patients receiving large doses of iodine-131 for the treatment of thyroid carcinoma. The physical estimate was based on the measurement of thyroid uptake, of the plasma activity variation, and of urinary activity. The cytogenetic estimate was obtained from the analysis of chromosome aberrations in peripheral blood lymphocytes. Good agreement between the estimates was observed in patients whose thyroid glands had previously been ablated by radioiodine. In patients who had varying degrees of thyroid function, there were considerable differences between the estimates with the cytogenetic value always being higher. It is suggested that these differences might be due in part to non-uniform irradiation of lymphocytes by local sources of activity in the thyroid and in the liver."}
{"id": "PMID:1087292", "title": "An analysis of the interaction of a platinum complex and radiation with CHO cells using the molecular theory of cell survival.", "content": "A mathematical analysis is developed from the concepts of the molecular theory of cell survival to explain the cytotoxic action of a platinum complex on CHO cells and its synergistic interaction with radiation. In the analysis, it is assumed that both inter- and intra-strand cross-links induced by the Pt complex in the DNA contribute to cell killing and that the synergistic effect arises from the interaction between an intra-strand cross-link and a radiation-induced single-strand break on complementary strands of the DNA. The analysis is shown to be compatible with experimental results. Further, a mechanistic model is developed in an attempt to explain in more detail the processes which appear to be involved in the expression of the cytological damage.", "contents": "An analysis of the interaction of a platinum complex and radiation with CHO cells using the molecular theory of cell survival. A mathematical analysis is developed from the concepts of the molecular theory of cell survival to explain the cytotoxic action of a platinum complex on CHO cells and its synergistic interaction with radiation. In the analysis, it is assumed that both inter- and intra-strand cross-links induced by the Pt complex in the DNA contribute to cell killing and that the synergistic effect arises from the interaction between an intra-strand cross-link and a radiation-induced single-strand break on complementary strands of the DNA. The analysis is shown to be compatible with experimental results. Further, a mechanistic model is developed in an attempt to explain in more detail the processes which appear to be involved in the expression of the cytological damage."}
{"id": "PMID:1087293", "title": "Skin response to X-irradiation in the guinea-pig.", "content": "Skin reaction to X-irradiation has been studied in the albino guinea-pig; early response in limited-field irradiations of the flank is comparable to that commonly seen in rodents, swine and man, and is dose-dependent with a dynamic range from mild erythema to moist desquamation. The peak early skin reaction is seen between 14 and 21 days after irradiation, and declines before 30 days except at the highest doses used. Fractionation of the X-ray dose at 24 hours results in a 'sparing' of about 340 rad. Permanent partial epilation is detectable at doses in excess of 1400 rad, and complete epilation at 1 year occurs in 50 per cent of irradiated fields at 1740 rad. Twenty-four hour two-dose fractionation results in a 'sparing' of about 500 rad for epilation. Palpable dermal 'fibrosis' is detectable at 3 months after irradiation in fields given more than 2070 rad, and at 1 year after irradiation in fields given more than 1800 rad; 50 per cent of fields showed palpable 'fibrosis' at 1 year at 1930 rad. Unlike domestic swine and man, skin fields in the guinea-pig showed no dimensional contraction after X-ray doses which produced gross early skin damage.", "contents": "Skin response to X-irradiation in the guinea-pig. Skin reaction to X-irradiation has been studied in the albino guinea-pig; early response in limited-field irradiations of the flank is comparable to that commonly seen in rodents, swine and man, and is dose-dependent with a dynamic range from mild erythema to moist desquamation. The peak early skin reaction is seen between 14 and 21 days after irradiation, and declines before 30 days except at the highest doses used. Fractionation of the X-ray dose at 24 hours results in a 'sparing' of about 340 rad. Permanent partial epilation is detectable at doses in excess of 1400 rad, and complete epilation at 1 year occurs in 50 per cent of irradiated fields at 1740 rad. Twenty-four hour two-dose fractionation results in a 'sparing' of about 500 rad for epilation. Palpable dermal 'fibrosis' is detectable at 3 months after irradiation in fields given more than 2070 rad, and at 1 year after irradiation in fields given more than 1800 rad; 50 per cent of fields showed palpable 'fibrosis' at 1 year at 1930 rad. Unlike domestic swine and man, skin fields in the guinea-pig showed no dimensional contraction after X-ray doses which produced gross early skin damage."}
{"id": "PMID:1087294", "title": "Effect of gamma-irradiation on dye-DNA binding.", "content": "The effect of gamma-rays on the binding of proflavine and acridine orange to DNA was investigated by spectrophotometry. The effect of irradiation was observed on the buffered solutions of the free dye and free DNA. A dose of about 35 krad caused a hyperchromicity of 30-40 per cent to the DNA peak at 258 nm, while the same dose introduced a hypochromic effect to the monomer peaks of the dyes by 30 per cent. This implied that gamma-rays have an effect of decreasing the monomer concentration of free-day molecules in solution. From the results, we conclude that more dye is bound to the changed conformation of dye-bound DNA on irradiation. Scratchard-binding isotherms drawn for the unirradiated and irradiated complexes of Pf-DNA showed interesting differences. Similar isotherms could not be obtained for the acridine orange-DNA system.", "contents": "Effect of gamma-irradiation on dye-DNA binding. The effect of gamma-rays on the binding of proflavine and acridine orange to DNA was investigated by spectrophotometry. The effect of irradiation was observed on the buffered solutions of the free dye and free DNA. A dose of about 35 krad caused a hyperchromicity of 30-40 per cent to the DNA peak at 258 nm, while the same dose introduced a hypochromic effect to the monomer peaks of the dyes by 30 per cent. This implied that gamma-rays have an effect of decreasing the monomer concentration of free-day molecules in solution. From the results, we conclude that more dye is bound to the changed conformation of dye-bound DNA on irradiation. Scratchard-binding isotherms drawn for the unirradiated and irradiated complexes of Pf-DNA showed interesting differences. Similar isotherms could not be obtained for the acridine orange-DNA system."}
{"id": "PMID:1087295", "title": "The relationship between early and late radiation damage in rodents' skin.", "content": "A study is made of the relationship between early and late damage after irradiation of the feet or ears of rats. In one type of experiment in which large groups of animals were given the same dose, there was a good correlation between the early and late forms of damage. In another experiment, the relationship between these two forms of radiation damage was found to be unaltered by a single dose of X-rays given 8 months earlier. Thus, both types of experiment point to the early and late forms of reaction in the skin of rodents resulting from a common cause. Other relevant experimental data and their relationship to clinical observations are discussed.", "contents": "The relationship between early and late radiation damage in rodents' skin. A study is made of the relationship between early and late damage after irradiation of the feet or ears of rats. In one type of experiment in which large groups of animals were given the same dose, there was a good correlation between the early and late forms of damage. In another experiment, the relationship between these two forms of radiation damage was found to be unaltered by a single dose of X-rays given 8 months earlier. Thus, both types of experiment point to the early and late forms of reaction in the skin of rodents resulting from a common cause. Other relevant experimental data and their relationship to clinical observations are discussed."}
{"id": "PMID:1087300", "title": "[Dynamics and metabolism in coronary heart disease before and after revascularization intervention].", "content": "In summary, rapid atrial pacing prior to surgery may be able to assess LVF and the degree of coronary insufficiency by measuring lactate production. Functional contraction abnormalities of the left ventricle are described by the anginal depression of the LVF, whereas a preanginal depression should be a sign of organic, fibrotic impairment of myocardial function. In cases of the former, after successful bypass surgery, a normal LVF may be observed. Moreover, myocardial metabolism is normalised. In the latter group, despite clinical improvement, functional abnormalities can persist.", "contents": "[Dynamics and metabolism in coronary heart disease before and after revascularization intervention]. In summary, rapid atrial pacing prior to surgery may be able to assess LVF and the degree of coronary insufficiency by measuring lactate production. Functional contraction abnormalities of the left ventricle are described by the anginal depression of the LVF, whereas a preanginal depression should be a sign of organic, fibrotic impairment of myocardial function. In cases of the former, after successful bypass surgery, a normal LVF may be observed. Moreover, myocardial metabolism is normalised. In the latter group, despite clinical improvement, functional abnormalities can persist."}
{"id": "PMID:1087299", "title": "Positive contrast ventriculography in cats with experimental obstructive hydrocephalus.", "content": "Cerebrospinal fluid pathways were studied in both normal and experimental obstructed hydrocephalic cats by positive contrast ventriculography. Either water soluble or insoluble contrast material was injected into the lateral cerebral ventricles, and radiographs were taken of the head and spinal cord. In the normal cat, the contrast material freely flowed throughout the spinal fluid spaces. The contrast material accumulated in the cisterna magna, and from there extended into the cranial and spinal subarachnoid spaces. In the kaolin-induced hydrocephalic cat, the outlets from the fourth ventricle were obstructed, and direct communication between the ventricular system and the subarachnoid spaces no longer existed. In these cats, the contrast material passed directly down the central canal of spinal cord and its movement was followed throughout the entire length of the canal. At the lower lumbar-sacral regions, the material perforated the cord and flowed into the subarachnoid space. At all levels, the central canal was enlarged and local dilatations were seen extending dorsally.", "contents": "Positive contrast ventriculography in cats with experimental obstructive hydrocephalus. Cerebrospinal fluid pathways were studied in both normal and experimental obstructed hydrocephalic cats by positive contrast ventriculography. Either water soluble or insoluble contrast material was injected into the lateral cerebral ventricles, and radiographs were taken of the head and spinal cord. In the normal cat, the contrast material freely flowed throughout the spinal fluid spaces. The contrast material accumulated in the cisterna magna, and from there extended into the cranial and spinal subarachnoid spaces. In the kaolin-induced hydrocephalic cat, the outlets from the fourth ventricle were obstructed, and direct communication between the ventricular system and the subarachnoid spaces no longer existed. In these cats, the contrast material passed directly down the central canal of spinal cord and its movement was followed throughout the entire length of the canal. At the lower lumbar-sacral regions, the material perforated the cord and flowed into the subarachnoid space. At all levels, the central canal was enlarged and local dilatations were seen extending dorsally."}
{"id": "PMID:1087304", "title": "The effects of response contingent vestibular stimulation on the behavior of autistic and retarded children.", "content": "The purpose of the present experiment was to examine the reinforcing effects of repetitive vestibular stimulation. While both autistic and retarded children would push a button for vestibular stimulation, frequency of stimulation was an important parameter for autistics but not for retardates. These results were interpreted as evidence for central rather than peripheral locus of control of motility disturbances in autistic children.", "contents": "The effects of response contingent vestibular stimulation on the behavior of autistic and retarded children. The purpose of the present experiment was to examine the reinforcing effects of repetitive vestibular stimulation. While both autistic and retarded children would push a button for vestibular stimulation, frequency of stimulation was an important parameter for autistics but not for retardates. These results were interpreted as evidence for central rather than peripheral locus of control of motility disturbances in autistic children."}
{"id": "PMID:1087306", "title": "Glucocorticoid action on rat thymic lymphocytes. Experiments utilizing adenosine to support cellular metabolism lead to a reassessment of catabolic hormone actions.", "content": "Inhibition of glucose uptake has been proposed as a primary cause of many of the subsequent inhibitory effects of glucocorticoids. This hypothesis has been tested in experiments where adenosine is substituted for glucose. Like glucose, adenosine maximally supports glycolytic and oxidative ATP generation, and by its use the hormonal inhibition of glucose uptake is circumvented. With adenosine, inhibition by cortisol is seen at at least one other metabolic site, respiratory ATP synthesis. This action can be observed by hormone-induced increases in levels of lactate, pyruvate, and AMP that accompany a lowering of ATP. Evidence for this metabolic action is also seen when cells are provided with a limiting amount of glucose; despite inhibition of glucose uptake, a cortisol-induced increase in lactate accompanies the reduction in levels of ATP. Decreased respiratory ATP synthesis is also suggested by a hormonal reduction in the metabolism of labeled exogenous pyruvate to 14CO2. Several experimental approaches suggest that inhibition of oxidative ATP production, rather than of glucose uptake, is the event most responsible for glucocorticoid-induced changes in the balance of adenine nucleotides, which in turn contribute to effects on protein synthesis and uridine uptake. First, the characteristic inhibitory cortisol effects on adenine nucleotides and protein synthesis are undiminished when adenosine is substituted for glucose. Second, in adenosine-supported cells the onset of the hormone-induced increase in levels of lactate corresponds closely to the appearance of measurable reductions in ATP. In contrast, when cells are supported by glucose, the hormonal inhibition of glucose uptake is maximal by 30 to 35 min, nearly an hour before effects on levels of ATP are detectable. Third, when cells are made strongly dependent upon glucose for ATP production by deprivation of exogenous substrate and cortisol is added at 90 min, a characteristic inhibition of the uptake of glucose added 40 min later is seen; nevertheless, this is insufficient to prevent added glucose from immediately and fully restoring ATP, rates of protein synthesis, and uridine uptake. Inhibitory effects on ATP, protein synthesis, and uridine do appear after an additional hour or so, a time commensurate with the development of an inhibition of oxidative metabolism. Fourth, limiting added glucose can reduce uptake more than cortisol, without reducing levels of ATP.", "contents": "Glucocorticoid action on rat thymic lymphocytes. Experiments utilizing adenosine to support cellular metabolism lead to a reassessment of catabolic hormone actions. Inhibition of glucose uptake has been proposed as a primary cause of many of the subsequent inhibitory effects of glucocorticoids. This hypothesis has been tested in experiments where adenosine is substituted for glucose. Like glucose, adenosine maximally supports glycolytic and oxidative ATP generation, and by its use the hormonal inhibition of glucose uptake is circumvented. With adenosine, inhibition by cortisol is seen at at least one other metabolic site, respiratory ATP synthesis. This action can be observed by hormone-induced increases in levels of lactate, pyruvate, and AMP that accompany a lowering of ATP. Evidence for this metabolic action is also seen when cells are provided with a limiting amount of glucose; despite inhibition of glucose uptake, a cortisol-induced increase in lactate accompanies the reduction in levels of ATP. Decreased respiratory ATP synthesis is also suggested by a hormonal reduction in the metabolism of labeled exogenous pyruvate to 14CO2. Several experimental approaches suggest that inhibition of oxidative ATP production, rather than of glucose uptake, is the event most responsible for glucocorticoid-induced changes in the balance of adenine nucleotides, which in turn contribute to effects on protein synthesis and uridine uptake. First, the characteristic inhibitory cortisol effects on adenine nucleotides and protein synthesis are undiminished when adenosine is substituted for glucose. Second, in adenosine-supported cells the onset of the hormone-induced increase in levels of lactate corresponds closely to the appearance of measurable reductions in ATP. In contrast, when cells are supported by glucose, the hormonal inhibition of glucose uptake is maximal by 30 to 35 min, nearly an hour before effects on levels of ATP are detectable. Third, when cells are made strongly dependent upon glucose for ATP production by deprivation of exogenous substrate and cortisol is added at 90 min, a characteristic inhibition of the uptake of glucose added 40 min later is seen; nevertheless, this is insufficient to prevent added glucose from immediately and fully restoring ATP, rates of protein synthesis, and uridine uptake. Inhibitory effects on ATP, protein synthesis, and uridine do appear after an additional hour or so, a time commensurate with the development of an inhibition of oxidative metabolism. Fourth, limiting added glucose can reduce uptake more than cortisol, without reducing levels of ATP."}
{"id": "PMID:1087310", "title": "Morphological changes in frog pronephric cell surfaces after transformation by herpes virus.", "content": "A scanning electron-microscope study has established that there are major modifications in the surface membranes of malignant cells transformed from the pronephric kidney of the frog. Tumours were induced in vivo after exposure of embryos Luck\u00e9 Herpes virus. A pronephric cell line from normal embryos and 3 tumour cell lines derived from experimentally induced adenocarcinomas of the pronephros were observed. The normal embryonic kidney cells have their surfaces covered with long fingerlike microvilli throughout the cell cycle. In contrast, the surface of the pronephric tumour cells are mainly covered by multiple, ruffled or parallel straight microridges and short, stubby microvilli. These comparisons were made on cells subject to similar culture conditions. The topographic changes in the tumour cells are considered significant since the characteristic microridges and stubby villi were consistently found in all 3 pronephric tumour lines.", "contents": "Morphological changes in frog pronephric cell surfaces after transformation by herpes virus. A scanning electron-microscope study has established that there are major modifications in the surface membranes of malignant cells transformed from the pronephric kidney of the frog. Tumours were induced in vivo after exposure of embryos Luck\u00e9 Herpes virus. A pronephric cell line from normal embryos and 3 tumour cell lines derived from experimentally induced adenocarcinomas of the pronephros were observed. The normal embryonic kidney cells have their surfaces covered with long fingerlike microvilli throughout the cell cycle. In contrast, the surface of the pronephric tumour cells are mainly covered by multiple, ruffled or parallel straight microridges and short, stubby microvilli. These comparisons were made on cells subject to similar culture conditions. The topographic changes in the tumour cells are considered significant since the characteristic microridges and stubby villi were consistently found in all 3 pronephric tumour lines."}
{"id": "PMID:1087313", "title": "A human T-independent B lymphocyte mitogen extracted from Nocardia opaca.", "content": "Nocardia water soluble mitogen (NWSM), a mitogen specific for mouse and rabbit B cells, activates blood, spleen, and tonsil lymphocytes from all the humans tested. Evidence is presented that it acts selectively on human B lymphocytes. B-enriched cells were recovered from anti-Ig-coated Sephadex columns or after elimination of E rosette-forming cells: they were able to incorporate tritiated thymidine when they were cultivated in the presence of NWSM whereas they were not activated by PWM. Conversely T-enriched suspensions did not respond to NWSM although they were able to be stimulated by PHA and PWM. Moreover, the response to NWSM remained unchanged after T cells had been killed by anti-HTLA serum and complement and disappeared after B cells had been killed by anti-HBLMA serum. After stimulation by NWSM, the number of direct plaque-forming cells to HRBC, LPS, and TNP was significantly increased, showing a polyclonal activation of human B lymphocytes. Since no evidence of T cell help for B cell activation has been found, NWSM thus appears to be a T-independent human B lymphocyte mitogen.", "contents": "A human T-independent B lymphocyte mitogen extracted from Nocardia opaca. Nocardia water soluble mitogen (NWSM), a mitogen specific for mouse and rabbit B cells, activates blood, spleen, and tonsil lymphocytes from all the humans tested. Evidence is presented that it acts selectively on human B lymphocytes. B-enriched cells were recovered from anti-Ig-coated Sephadex columns or after elimination of E rosette-forming cells: they were able to incorporate tritiated thymidine when they were cultivated in the presence of NWSM whereas they were not activated by PWM. Conversely T-enriched suspensions did not respond to NWSM although they were able to be stimulated by PHA and PWM. Moreover, the response to NWSM remained unchanged after T cells had been killed by anti-HTLA serum and complement and disappeared after B cells had been killed by anti-HBLMA serum. After stimulation by NWSM, the number of direct plaque-forming cells to HRBC, LPS, and TNP was significantly increased, showing a polyclonal activation of human B lymphocytes. Since no evidence of T cell help for B cell activation has been found, NWSM thus appears to be a T-independent human B lymphocyte mitogen."}
{"id": "PMID:1087314", "title": "The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity.", "content": "The identity of the effector cells in human peripheral blood capable of mediating mitogen-induced cellular cytotoxicity (MICC) and antibody-dependent cellular cytotoxicity (ADCC) was investigated utilizing effector cell populations consisting of purified polymorphonuclear leukocytes, macrophages, lymphocytes, and cell surface immunoglobulin (sIg)-negative and sIg-positive lymphocyte subpopulations obtained by Sephadex anti-Fab immunoabsorbent column fractionation techniques. Chicken erythrocytes (CRBC) and Chang liver cells were used as target cells in both cytotoxicity assays. With CRBC targets MICC was mediated by polymorphonuclear leukocytes, macrophages, sIg-positive lymphocytes (B cells), and sIg-negative lymphocytes. On the contrary, with Chang liver cells as targets, MICC was mediated only by lymphocytes, and effector cells occurred exclusively in sIg-negative lymphocyte subpopulations containing thymus-derived lymphocytes (T cells). Further purification of sIg-negative lymphocyte subpopulations on antigen-antibody coated plastic surfaces yielded a nonadherent T lymphocyte population depleted of Fc receptor-bearing lymphocytes that was capable of mediating MICC against both CRBC and Chang cell targets. With use of CRBC targets, ADCC was mediated by polymorphonuclear leukocytes, macrophages, and sIg-negative lymphocyte subpopulations. However, with Chang cell targets, ADCC was mediated only by lymphocytes, and effector cells were present only in sIg-negative lymphocyte subpopulations. SIg-positive lymphocytes (B cells) and T lymphocytes were not effective in mediating ADCC against either CRBC or Chang cell targets. These studies demonstrate that the nature of the target cell employed in MICC and ADCC reactions is of critical imporatnce in defining the effector cell(s) capable of mediating cytotoxicity.", "contents": "The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity. The identity of the effector cells in human peripheral blood capable of mediating mitogen-induced cellular cytotoxicity (MICC) and antibody-dependent cellular cytotoxicity (ADCC) was investigated utilizing effector cell populations consisting of purified polymorphonuclear leukocytes, macrophages, lymphocytes, and cell surface immunoglobulin (sIg)-negative and sIg-positive lymphocyte subpopulations obtained by Sephadex anti-Fab immunoabsorbent column fractionation techniques. Chicken erythrocytes (CRBC) and Chang liver cells were used as target cells in both cytotoxicity assays. With CRBC targets MICC was mediated by polymorphonuclear leukocytes, macrophages, sIg-positive lymphocytes (B cells), and sIg-negative lymphocytes. On the contrary, with Chang liver cells as targets, MICC was mediated only by lymphocytes, and effector cells occurred exclusively in sIg-negative lymphocyte subpopulations containing thymus-derived lymphocytes (T cells). Further purification of sIg-negative lymphocyte subpopulations on antigen-antibody coated plastic surfaces yielded a nonadherent T lymphocyte population depleted of Fc receptor-bearing lymphocytes that was capable of mediating MICC against both CRBC and Chang cell targets. With use of CRBC targets, ADCC was mediated by polymorphonuclear leukocytes, macrophages, and sIg-negative lymphocyte subpopulations. However, with Chang cell targets, ADCC was mediated only by lymphocytes, and effector cells were present only in sIg-negative lymphocyte subpopulations. SIg-positive lymphocytes (B cells) and T lymphocytes were not effective in mediating ADCC against either CRBC or Chang cell targets. These studies demonstrate that the nature of the target cell employed in MICC and ADCC reactions is of critical imporatnce in defining the effector cell(s) capable of mediating cytotoxicity."}
{"id": "PMID:1087315", "title": "Identification of multiple cytolytic components associated with the beta-LT class of lymphotoxins released by mitogen-activity human lymphocytes in vitro.", "content": "Cytotoxic activity (lymphotoxin (LT) associated with the 50,000 dalton beta-LT class of human lymphotoxins has been resolved into multiple components by chromatography on DEAE-cellulose or electrophoresis on polyacrylamide gels. The two components, termed beta-LT, and beta-LT2, are separable from the 90,000 m.w. alpha-LT, since neither component is neutralized by incubation with heterologous rabbit anti-alpha-LT antisera. However, the two components differ in their overall charge and relative heat stability with the betaLT1 component being more positively charged than the beat-LT2 molecule and extremely heat labile.", "contents": "Identification of multiple cytolytic components associated with the beta-LT class of lymphotoxins released by mitogen-activity human lymphocytes in vitro. Cytotoxic activity (lymphotoxin (LT) associated with the 50,000 dalton beta-LT class of human lymphotoxins has been resolved into multiple components by chromatography on DEAE-cellulose or electrophoresis on polyacrylamide gels. The two components, termed beta-LT, and beta-LT2, are separable from the 90,000 m.w. alpha-LT, since neither component is neutralized by incubation with heterologous rabbit anti-alpha-LT antisera. However, the two components differ in their overall charge and relative heat stability with the betaLT1 component being more positively charged than the beat-LT2 molecule and extremely heat labile."}
{"id": "PMID:1087316", "title": "The induction of hapten-specific T cell tolerance by using hapten-modified lymphoid cells. I. Characteristics of tolerance induction.", "content": "BALB/c mice were made tolerant to the T cell-dependent phenomenon of contact sensitivity to DNFB by i.v. injection of syngeneic lymphoid cells which had been previously modified with DNFB in vitro. The highly efficient unresponsiveness, as measured by ear challenge and in vitro antigen-induced cell proliferation, was shown to follow dose-response kinetics both in vivo and in vitro and to be exquisitely specific for the DNP moiety. The kinetics of tolerance induction were shown to be very rapid and had been previously shown to be long lasting. Unresponsiveness was more efficient when the hapten-modified cells were introduced by the i.v. route and tolerance could be increased by repeated injection of tolerogen. The tolerance could be transferred to normal syngeneic recipients by spleen and/or lymph node cells from tolerant donors. A wide variety of hapten-modified lymphoid cells, including mixed cell populations and enriched populations of T cells, B cells, and macrophages, were capable of inducing tolerance. The unresponsiveness was dependent  merely on the association of DNP to the lymphoid membrane proteins and not upon the viability of the hapten-modified cells. These experiments support the hypothesis that in hapten-specific T cell sensitivity, as exemplified by contact sensitivity to DNFB, specific T cell tolerance in actively induced by hapten on self-membrane. In other hapten-specific antibody-forming systems, tolerance appears to be most readily induced by hapten on soluble self protein or on a nonimmunogeneic carrier.", "contents": "The induction of hapten-specific T cell tolerance by using hapten-modified lymphoid cells. I. Characteristics of tolerance induction. BALB/c mice were made tolerant to the T cell-dependent phenomenon of contact sensitivity to DNFB by i.v. injection of syngeneic lymphoid cells which had been previously modified with DNFB in vitro. The highly efficient unresponsiveness, as measured by ear challenge and in vitro antigen-induced cell proliferation, was shown to follow dose-response kinetics both in vivo and in vitro and to be exquisitely specific for the DNP moiety. The kinetics of tolerance induction were shown to be very rapid and had been previously shown to be long lasting. Unresponsiveness was more efficient when the hapten-modified cells were introduced by the i.v. route and tolerance could be increased by repeated injection of tolerogen. The tolerance could be transferred to normal syngeneic recipients by spleen and/or lymph node cells from tolerant donors. A wide variety of hapten-modified lymphoid cells, including mixed cell populations and enriched populations of T cells, B cells, and macrophages, were capable of inducing tolerance. The unresponsiveness was dependent  merely on the association of DNP to the lymphoid membrane proteins and not upon the viability of the hapten-modified cells. These experiments support the hypothesis that in hapten-specific T cell sensitivity, as exemplified by contact sensitivity to DNFB, specific T cell tolerance in actively induced by hapten on self-membrane. In other hapten-specific antibody-forming systems, tolerance appears to be most readily induced by hapten on soluble self protein or on a nonimmunogeneic carrier."}
{"id": "PMID:1087317", "title": "Cell surface immunoglobulin. XIX. Susceptibility of IgD and IgM on murine splenocytes to cleavage by papain.", "content": "The present studies were designed to determine the effect of papain upon IgM and IgD on the surface of murine splenocytes. By using radioiodinated cells, it was shown that IgD is more susceptible to cleavage by papain than IgM. The difference in susceptibility to papain of the two isotypes was not observed when cell lysates were employed, suggesting that attachment to the plasma membrane affects the accessibility of IgM to papain. The major site of attack of the enzyme on IgD appears to be in the hinge region. The possible biologic implications of such a cleavage are discussed.", "contents": "Cell surface immunoglobulin. XIX. Susceptibility of IgD and IgM on murine splenocytes to cleavage by papain. The present studies were designed to determine the effect of papain upon IgM and IgD on the surface of murine splenocytes. By using radioiodinated cells, it was shown that IgD is more susceptible to cleavage by papain than IgM. The difference in susceptibility to papain of the two isotypes was not observed when cell lysates were employed, suggesting that attachment to the plasma membrane affects the accessibility of IgM to papain. The major site of attack of the enzyme on IgD appears to be in the hinge region. The possible biologic implications of such a cleavage are discussed."}
{"id": "PMID:1087318", "title": "The induction and regulation of guinea pig B-lymphocyte proliferation in vitro.", "content": "Conditions for the in vitro induction of proliferation have been examined in a population of guinea pig lymph node lymphocytes enriched for B lymphocytes. Known B cell mitogens such as bacterial lipopolysaccharide and tuberculin-purified protein derivative induced DNA synthetic responses in this B lymphocyte population as did a number of dinitrophenyl hapten-protein conjugates, all in the apparent absence of T cell participation. B cell proliferation occurred most efficiently in serum-free medium with both heterologous and homologous sera inhibiting maximum responses. In vivo immunization with hapten-protein conjugates failed to enhance subsequent in vitro B cell responsiveness to these materials beyond that owing to recruitment by contaminating antigen-primed T cells. Macrophage-associated antigen, which efficiently triggered T cell proliferation was much less effective at initiating B cell DNA synthesis than was soluble antigen not bound to macrophages. Furthermore, although accessory cells were required for the development of T cell DNA synthetic responses, macrophages of factors produced by them inhibited spontaneous or induced B cell 3H-thymidine incorporation.", "contents": "The induction and regulation of guinea pig B-lymphocyte proliferation in vitro. Conditions for the in vitro induction of proliferation have been examined in a population of guinea pig lymph node lymphocytes enriched for B lymphocytes. Known B cell mitogens such as bacterial lipopolysaccharide and tuberculin-purified protein derivative induced DNA synthetic responses in this B lymphocyte population as did a number of dinitrophenyl hapten-protein conjugates, all in the apparent absence of T cell participation. B cell proliferation occurred most efficiently in serum-free medium with both heterologous and homologous sera inhibiting maximum responses. In vivo immunization with hapten-protein conjugates failed to enhance subsequent in vitro B cell responsiveness to these materials beyond that owing to recruitment by contaminating antigen-primed T cells. Macrophage-associated antigen, which efficiently triggered T cell proliferation was much less effective at initiating B cell DNA synthesis than was soluble antigen not bound to macrophages. Furthermore, although accessory cells were required for the development of T cell DNA synthetic responses, macrophages of factors produced by them inhibited spontaneous or induced B cell 3H-thymidine incorporation."}
{"id": "PMID:1087319", "title": "Ontogeny of the antibody-forming cell line in mice. II. Maturation of B cells during fetal development.", "content": "This paper maps the developmental stage reached by B cells in mouse fetuses of different ages. The \"maturity\" of these cells was assessed in a functional way by measuring the time taken for cells to generate antibody-forming progeny when injected into carrier-primed irradiated adult hosts and challenged daily with antigen. A steady uninterrupted progression in development of B cells occurred between 13 days of gestation and birth. There was no evidence for a stage during which B cells could only be paralyzed and not induced by antigen.", "contents": "Ontogeny of the antibody-forming cell line in mice. II. Maturation of B cells during fetal development. This paper maps the developmental stage reached by B cells in mouse fetuses of different ages. The \"maturity\" of these cells was assessed in a functional way by measuring the time taken for cells to generate antibody-forming progeny when injected into carrier-primed irradiated adult hosts and challenged daily with antigen. A steady uninterrupted progression in development of B cells occurred between 13 days of gestation and birth. There was no evidence for a stage during which B cells could only be paralyzed and not induced by antigen."}
{"id": "PMID:1087320", "title": "Hapten specific IgE antibody responses in mice. V. Differential resistance of IgE and IgG B lymphocytes to X-irradiation.", "content": "The studies presented in this paper were undertaken to determine whether IgE- and IgG-producing murine B lymphocytes could be distinguished by differential resistance to x-irradiation in vivo. Sensitivity or resistance of B lymphocytes to various doses of x-irradiation was monitored by measuring the production by irradiated cells of hapten-specific IgG and IgE antibody. The results indicate that both primed and unprimed IgG B lymphocytes are less radioresistant than corresponding IgE B lymphocytes. Primed IgG B lymphocytes are more radioresistant than unprimed IgG B cells whereas unprimed IgE B lymphocytes are at least as radioresistant as primed IgE B lymphocytes. The relative radioresistance of primed IgE vs IgG B cells was best demonstrated in an adoptive transfer system. Thus, exposure of ASC-primed mice to 300 R 24 hr after transfer into these mice of syngeneic, DNP-KLH-primed spleen cells led to a sustained failure to produce IgG anti-DNP antibody after challenge with DNP-ASC; under these conditions IgE anti-DNP production was not impaired. This was shown to reflect differential radioresistance of the corresponding B cell populations. In addition, exposure to x-irradiation tended to augment IgE anti-DNP antibody responses when compared to unirradiated controls. This phenomenon is thought to reflect the effects of x-irradiation on regulatory mechanisms controlling the mouse IgE response and is explored in detail in an accompanying manuscript. The results described in this paper are discussed in the light of our evidence suggesting that, in both mouse and man, IgE B lymphocytes have certain unique characteristics by which they may be distinguished from other classes of B lymphocytes.", "contents": "Hapten specific IgE antibody responses in mice. V. Differential resistance of IgE and IgG B lymphocytes to X-irradiation. The studies presented in this paper were undertaken to determine whether IgE- and IgG-producing murine B lymphocytes could be distinguished by differential resistance to x-irradiation in vivo. Sensitivity or resistance of B lymphocytes to various doses of x-irradiation was monitored by measuring the production by irradiated cells of hapten-specific IgG and IgE antibody. The results indicate that both primed and unprimed IgG B lymphocytes are less radioresistant than corresponding IgE B lymphocytes. Primed IgG B lymphocytes are more radioresistant than unprimed IgG B cells whereas unprimed IgE B lymphocytes are at least as radioresistant as primed IgE B lymphocytes. The relative radioresistance of primed IgE vs IgG B cells was best demonstrated in an adoptive transfer system. Thus, exposure of ASC-primed mice to 300 R 24 hr after transfer into these mice of syngeneic, DNP-KLH-primed spleen cells led to a sustained failure to produce IgG anti-DNP antibody after challenge with DNP-ASC; under these conditions IgE anti-DNP production was not impaired. This was shown to reflect differential radioresistance of the corresponding B cell populations. In addition, exposure to x-irradiation tended to augment IgE anti-DNP antibody responses when compared to unirradiated controls. This phenomenon is thought to reflect the effects of x-irradiation on regulatory mechanisms controlling the mouse IgE response and is explored in detail in an accompanying manuscript. The results described in this paper are discussed in the light of our evidence suggesting that, in both mouse and man, IgE B lymphocytes have certain unique characteristics by which they may be distinguished from other classes of B lymphocytes."}
{"id": "PMID:1087321", "title": "Hapten-specific IgE antibody responses in mice. VI. Selective enhancement of IgE antibody production by low doses of X-irradiation and by cyclophosphamide.", "content": "Exposure of BALB/c, A/J, and (BALB/c X A/J)F1 mice, which are IgE high responder animals, to total body x-irradiation ranging from 50 to 200 R resulted in a dose-dependent enhancement of the hapten-specific IgE antibody level as compared to unirradiated control mice. In contrast, anti-hapten antibody responses of the IgG class in these same animals were rarely enhanced, and when so, were of a lesser degree. This relatively selective augmentation of the IgE vs IgG antibody responses was observed in both unprimed and primed mice. By utilizing adoptive transfer systems, it was demonstrated that the enhancing effects of x-irradiation resulted from its action on the carrier-primed cell population and not upon the responding B cells or upon macrophages. The data presented herein suggest that this enhancement phenomenon is the result of the elimination of T cells (or their products) with suppressive functions and that these cells are neither dependent upon nor specific for the carrier antigen employed in the immunization. This hypothesis is given indirect support by the observations that treatment of the same strains of mice with cyclophosphamide, in doses known to abrogate suppressive T cell functions, resulted in a similar enhancing effect to that observed after low doses of x-irradiation. In addition an interesting difference between IgE and IgG precursor B lymphocytes was observed by the ability of IgE B cells to differentiate to the secretory state at a strikingly more rapid rate than IgG B lymphocytes when exposed to comparable T cell helper influences. These observations may provide important clues to the cellular mechanisms of the immune regulation of the IgE response and its relationship to allergic diseases.", "contents": "Hapten-specific IgE antibody responses in mice. VI. Selective enhancement of IgE antibody production by low doses of X-irradiation and by cyclophosphamide. Exposure of BALB/c, A/J, and (BALB/c X A/J)F1 mice, which are IgE high responder animals, to total body x-irradiation ranging from 50 to 200 R resulted in a dose-dependent enhancement of the hapten-specific IgE antibody level as compared to unirradiated control mice. In contrast, anti-hapten antibody responses of the IgG class in these same animals were rarely enhanced, and when so, were of a lesser degree. This relatively selective augmentation of the IgE vs IgG antibody responses was observed in both unprimed and primed mice. By utilizing adoptive transfer systems, it was demonstrated that the enhancing effects of x-irradiation resulted from its action on the carrier-primed cell population and not upon the responding B cells or upon macrophages. The data presented herein suggest that this enhancement phenomenon is the result of the elimination of T cells (or their products) with suppressive functions and that these cells are neither dependent upon nor specific for the carrier antigen employed in the immunization. This hypothesis is given indirect support by the observations that treatment of the same strains of mice with cyclophosphamide, in doses known to abrogate suppressive T cell functions, resulted in a similar enhancing effect to that observed after low doses of x-irradiation. In addition an interesting difference between IgE and IgG precursor B lymphocytes was observed by the ability of IgE B cells to differentiate to the secretory state at a strikingly more rapid rate than IgG B lymphocytes when exposed to comparable T cell helper influences. These observations may provide important clues to the cellular mechanisms of the immune regulation of the IgE response and its relationship to allergic diseases."}
{"id": "PMID:1087322", "title": "Contrasting effects of BCG on spleen and lymph node antibody responses in nude and normal mice.", "content": "Subcutaneous footpad injection of BCG causes a marked augmentation of popliteal lymph node plaque-forming cell (PFC) responses to DNP-derivatized hemocyanin and to sheep red blood cells, both T-dependent antigens, but not to DNP-derivatized Ficoll, a T-independent antigen. This augmentative effect occurs in normal thymus-bearing heterozygous (nu/+) mice, but not in congenitally athymic nude mice (nu/nu). In contrast, intravenous injection of BCG causes a suppressed splenic PFC response to subsequently administered T-dependent or T-independent antigens in both nude and nu/+ mice. BCG's augmenting effect on the lymph node appears to be mediated by a T-helper cell. BCG's suppressive effect in the spleen is not attributable to T cells. The actual mechanism of the BCG-mediated suppressive splenic effect remains incompletely defined at present.", "contents": "Contrasting effects of BCG on spleen and lymph node antibody responses in nude and normal mice. Subcutaneous footpad injection of BCG causes a marked augmentation of popliteal lymph node plaque-forming cell (PFC) responses to DNP-derivatized hemocyanin and to sheep red blood cells, both T-dependent antigens, but not to DNP-derivatized Ficoll, a T-independent antigen. This augmentative effect occurs in normal thymus-bearing heterozygous (nu/+) mice, but not in congenitally athymic nude mice (nu/nu). In contrast, intravenous injection of BCG causes a suppressed splenic PFC response to subsequently administered T-dependent or T-independent antigens in both nude and nu/+ mice. BCG's augmenting effect on the lymph node appears to be mediated by a T-helper cell. BCG's suppressive effect in the spleen is not attributable to T cells. The actual mechanism of the BCG-mediated suppressive splenic effect remains incompletely defined at present."}
{"id": "PMID:1087323", "title": "Abnormalities of cellular immune response in arthritis induced by rubella vaccination.", "content": "The rubella-specific antibody, activity the levels of immunoglobulins and complement in the serum, the number of T cells, and the nature of cell-mediated immunity as measured by lymphocyte transformation (LTF) of circulating lymphocytes in response to rubella virus and phyto-hemagglutinin (PHA) were studied in 15 children who manifested arthralgia or arthritis after parenteral immunization with HPV-77 DE/5 or RA27/3 live attenuated rubella virus vaccines. Eighteen appropriately matched subjects who developed no post-vaccine complications were included as controls. The immunoglobulins, complement, and rubella antibody levels were similar in all subjects. Although significant rubella specific cell-mediated immunity was detectable in most control subjects, the activity was markedly depressed in subjects with arthritis. The LTF activity in response to PHA was approximately equal in both groups. These findings suggest a selective depression of cell-mediated immunity to rubella virus in subjects with arthritis associated with rubella vaccination.", "contents": "Abnormalities of cellular immune response in arthritis induced by rubella vaccination. The rubella-specific antibody, activity the levels of immunoglobulins and complement in the serum, the number of T cells, and the nature of cell-mediated immunity as measured by lymphocyte transformation (LTF) of circulating lymphocytes in response to rubella virus and phyto-hemagglutinin (PHA) were studied in 15 children who manifested arthralgia or arthritis after parenteral immunization with HPV-77 DE/5 or RA27/3 live attenuated rubella virus vaccines. Eighteen appropriately matched subjects who developed no post-vaccine complications were included as controls. The immunoglobulins, complement, and rubella antibody levels were similar in all subjects. Although significant rubella specific cell-mediated immunity was detectable in most control subjects, the activity was markedly depressed in subjects with arthritis. The LTF activity in response to PHA was approximately equal in both groups. These findings suggest a selective depression of cell-mediated immunity to rubella virus in subjects with arthritis associated with rubella vaccination."}
{"id": "PMID:1087324", "title": "Anatomical observations on the distal extremity of the vestibular aqueduct.", "content": "A hitherto unobserved channel was observed, opening from the bony vestibular aqueduct under the dura. The location was in the centre between the contact of the aqueduct with the bulbar wall and the entrance of the aqueduct into the saccus. No connection was found between this formation and the vascular channels leading into the aqueduct or with the 'paravestibular canaliculus'. Albeit some of the examples were taken from Meni\u00e8re cases no interpretation in this direction was attempted. Strategical location claims further consideration as to morphology and function.", "contents": "Anatomical observations on the distal extremity of the vestibular aqueduct. A hitherto unobserved channel was observed, opening from the bony vestibular aqueduct under the dura. The location was in the centre between the contact of the aqueduct with the bulbar wall and the entrance of the aqueduct into the saccus. No connection was found between this formation and the vascular channels leading into the aqueduct or with the 'paravestibular canaliculus'. Albeit some of the examples were taken from Meni\u00e8re cases no interpretation in this direction was attempted. Strategical location claims further consideration as to morphology and function."}
{"id": "PMID:1087326", "title": "Induction and abrogation of unresponsiveness in nude mouse cells.", "content": "Nude mice were injected with antigen and T cells at different times to induce unresponsiveness to SRBC. Spleen cells derived from these mice were tested in vitro for the capability to produce antibody-forming cells against sheep erythrocytes in the presence of a T-cell-replacing factor. It was found that priming with antigen alone did not result in paralysis but a later injection of thymus-derived lymphocytes together with antigen results in unresponsiveness of these cells in vitro, provided there was an interval of several days between the in vivo administration of thymus lymphocytes and the explantations of cells to in vitro cultures.", "contents": "Induction and abrogation of unresponsiveness in nude mouse cells. Nude mice were injected with antigen and T cells at different times to induce unresponsiveness to SRBC. Spleen cells derived from these mice were tested in vitro for the capability to produce antibody-forming cells against sheep erythrocytes in the presence of a T-cell-replacing factor. It was found that priming with antigen alone did not result in paralysis but a later injection of thymus-derived lymphocytes together with antigen results in unresponsiveness of these cells in vitro, provided there was an interval of several days between the in vivo administration of thymus lymphocytes and the explantations of cells to in vitro cultures."}
{"id": "PMID:1087327", "title": "Cross-reactive lysis of trinitrophenyl (TNP)-derivatized H-2 incompatible target cells by cytolytic T lymphocytes generated against syngeneic TNP spleen cells.", "content": "Normal spleen cells, when cultured with irradiated trinitrophenyl (TNP)-derivatized syngeneic spleen cells, develop cytotoxic effectors that lyse most effectiviely a TNP-derivatized target that is H-2 compatible with the effector. However, these effectors also lyse to a lesser extent TNP tumor and TNP spleen targets that are H-2 incompatible. This cross-reactive lysis correlates with the degree of cytolysis seen on the TNP-derivatized syngeneic target; it appears to be medicated by Thy 1.2-bearing cells and is inhibited by antisera to the K and/or D loci of the target cell and not by antisera to non-K or non-D surface antigens. Nonradiolabeled TNP-derivatized lymphoid cells syngeneic to either the stimulator or the target are able to competitively inhibit cross-reactive lysis, while TNP chicken red blood cells are unable to specifically inhibit lysis. These data on cross-reactive lysis of TNP-conjugated targets are most consistent with the altered-self hypothesis.", "contents": "Cross-reactive lysis of trinitrophenyl (TNP)-derivatized H-2 incompatible target cells by cytolytic T lymphocytes generated against syngeneic TNP spleen cells. Normal spleen cells, when cultured with irradiated trinitrophenyl (TNP)-derivatized syngeneic spleen cells, develop cytotoxic effectors that lyse most effectiviely a TNP-derivatized target that is H-2 compatible with the effector. However, these effectors also lyse to a lesser extent TNP tumor and TNP spleen targets that are H-2 incompatible. This cross-reactive lysis correlates with the degree of cytolysis seen on the TNP-derivatized syngeneic target; it appears to be medicated by Thy 1.2-bearing cells and is inhibited by antisera to the K and/or D loci of the target cell and not by antisera to non-K or non-D surface antigens. Nonradiolabeled TNP-derivatized lymphoid cells syngeneic to either the stimulator or the target are able to competitively inhibit cross-reactive lysis, while TNP chicken red blood cells are unable to specifically inhibit lysis. These data on cross-reactive lysis of TNP-conjugated targets are most consistent with the altered-self hypothesis."}
{"id": "PMID:1087328", "title": "Spontaneous redistribution of surface immunoglobulin in the motile B lymphocyte.", "content": "This paper reports that B cells undergoing translatory motion spontaneously segregate their surface Ig to one portion of their plasma membrane. The spontaneous redistribution of surface Ig was found to be: (a) selective, concanavalin A-dependent on translatory motion and energy metabolism. Abundant B cells undergoing motility were found after cultures in lipopolysaccharide or trypsin, or after brief exposure to cholinergic drugs.", "contents": "Spontaneous redistribution of surface immunoglobulin in the motile B lymphocyte. This paper reports that B cells undergoing translatory motion spontaneously segregate their surface Ig to one portion of their plasma membrane. The spontaneous redistribution of surface Ig was found to be: (a) selective, concanavalin A-dependent on translatory motion and energy metabolism. Abundant B cells undergoing motility were found after cultures in lipopolysaccharide or trypsin, or after brief exposure to cholinergic drugs."}
{"id": "PMID:1087329", "title": "Multiple H-2 linked immune response gene control of H-2 D-associated T-cell-mediated lympholysis to trinitrophenyl-modified autologous cells: Ir-like genes mapping to the left of I-A and within the I region.", "content": "One of the more recent associations of the murine H-2 major histocompatibility complex (MHC) with immune function has been the finding that cytotoxic T-effector cells generated by sensitization with viral-infected (1-6), chemically modified (7-9), or weak transplantation antigen-associated (10,11) syngeneic cells can efficiently lyse target cells which express the same viral, chemical, or weak antigenic agent, and which share the H-2K and/or H-2D regions of the MHC with the responding and/or stimulating cells. Furthermore, an additional contribution of a gene(s) within the H-2 complex has been demonstrated which controls immune response potential (Ir genes) in the generation of cytotoxic effector cells to trinitrophenyl (TNP)-modified self components (12,13). In such studies it was found that certain B10 congenic strains generated good cytotoxic responses to both TNP- modified H-2K and H-2D region products, whereas other B10 congenic strains exhibited preferential or exclusive reactivity against TNP-modified H-2K region products. Some of these recombinant strains differing in response potential to TNP- modified H-2D products expressed the same haplotype at the D end, but differed at the K end of H-2. The low responsiveness observed in the B10.A strain to TNP-modified H-2D(d) when compared to B10.D2 and (B10.A x B10.D2)F(1) for the same specificity, suggested a role of dominant Ir genes which map in K, I-A, I-B, I-J, and/or I-E (12, 14). In the present report an attemnpt was made to further map within the MHC the Ir gene(s) controlling cell-mediated lympholysis (CML) to TNP-modified H-2D(d), by using recombinant mouse strains on the A and B10 backgrounds. Irrespective of the genetic background, the s and k haplotypes at the K end generated high and low cytotoxic responses, respectively, to H-2D(d)-TNP. The intermediate responder and low responder status of the A.TL and A.AL strains, respectively, indicated that a gene mapping in the K region of H-2 influences response potential. Furthermore, the differences in the levels of cytotoxicity detected in the A.TH and A.TL strains suggested an additional I region influence. Taken together these findings raise the possibility that multiple genes mapping within different regions of the MHC control the level of T-cell-mediated cytotoxicity to chemically modified autologous cells.", "contents": "Multiple H-2 linked immune response gene control of H-2 D-associated T-cell-mediated lympholysis to trinitrophenyl-modified autologous cells: Ir-like genes mapping to the left of I-A and within the I region. One of the more recent associations of the murine H-2 major histocompatibility complex (MHC) with immune function has been the finding that cytotoxic T-effector cells generated by sensitization with viral-infected (1-6), chemically modified (7-9), or weak transplantation antigen-associated (10,11) syngeneic cells can efficiently lyse target cells which express the same viral, chemical, or weak antigenic agent, and which share the H-2K and/or H-2D regions of the MHC with the responding and/or stimulating cells. Furthermore, an additional contribution of a gene(s) within the H-2 complex has been demonstrated which controls immune response potential (Ir genes) in the generation of cytotoxic effector cells to trinitrophenyl (TNP)-modified self components (12,13). In such studies it was found that certain B10 congenic strains generated good cytotoxic responses to both TNP- modified H-2K and H-2D region products, whereas other B10 congenic strains exhibited preferential or exclusive reactivity against TNP-modified H-2K region products. Some of these recombinant strains differing in response potential to TNP- modified H-2D products expressed the same haplotype at the D end, but differed at the K end of H-2. The low responsiveness observed in the B10.A strain to TNP-modified H-2D(d) when compared to B10.D2 and (B10.A x B10.D2)F(1) for the same specificity, suggested a role of dominant Ir genes which map in K, I-A, I-B, I-J, and/or I-E (12, 14). In the present report an attemnpt was made to further map within the MHC the Ir gene(s) controlling cell-mediated lympholysis (CML) to TNP-modified H-2D(d), by using recombinant mouse strains on the A and B10 backgrounds. Irrespective of the genetic background, the s and k haplotypes at the K end generated high and low cytotoxic responses, respectively, to H-2D(d)-TNP. The intermediate responder and low responder status of the A.TL and A.AL strains, respectively, indicated that a gene mapping in the K region of H-2 influences response potential. Furthermore, the differences in the levels of cytotoxicity detected in the A.TH and A.TL strains suggested an additional I region influence. Taken together these findings raise the possibility that multiple genes mapping within different regions of the MHC control the level of T-cell-mediated cytotoxicity to chemically modified autologous cells."}
{"id": "PMID:1087330", "title": "Cooperation across the histocompatibility barrier: H2d T cells primed to antigen in an H-2d environment can cooperate with H-2k B cells.", "content": "H-2d spleen cells derived from either tetraparental or semiallogeneic radiation bone marrow chimeras can be primed to antigen within H-2d recipients to generate helper T cells capable of cooperating in a secondary response with equal efficiency with H-2d or H-2k B cells. Thus it would seem that the cooperative act between T and B cells does not require that the T cell interacts with its target B cells by either cell interaction genes or via an altered self mechanism involving both antigen and the target B-cell I-region products. This does not preclude a requirement for associative recognition or altered self in the interaction of helper T cells with accessory cells.", "contents": "Cooperation across the histocompatibility barrier: H2d T cells primed to antigen in an H-2d environment can cooperate with H-2k B cells. H-2d spleen cells derived from either tetraparental or semiallogeneic radiation bone marrow chimeras can be primed to antigen within H-2d recipients to generate helper T cells capable of cooperating in a secondary response with equal efficiency with H-2d or H-2k B cells. Thus it would seem that the cooperative act between T and B cells does not require that the T cell interacts with its target B cells by either cell interaction genes or via an altered self mechanism involving both antigen and the target B-cell I-region products. This does not preclude a requirement for associative recognition or altered self in the interaction of helper T cells with accessory cells."}
{"id": "PMID:1087331", "title": "Daily variations in the effects of disturbance on growth, fattening, and metamorphosis in the bullfrog (Rana catesbeiana) tadpole.", "content": "Bullfrog tadpoles subjected to the daily disturbance of water replacement demonstrated differences in the rates of growth and metamorphosis and in fatbody weights which depended on the time of day when water replacement occurred. Early daily disturbances stimulated larval growth whereas midday disturbances stimulated metamorphosis. The time when the daily disturbances caused fattening changed during metamorphosis so that younger tadpoles fattened when disturbed early in the photoperiod and older tadpoles fattened when disturbed at midday.", "contents": "Daily variations in the effects of disturbance on growth, fattening, and metamorphosis in the bullfrog (Rana catesbeiana) tadpole. Bullfrog tadpoles subjected to the daily disturbance of water replacement demonstrated differences in the rates of growth and metamorphosis and in fatbody weights which depended on the time of day when water replacement occurred. Early daily disturbances stimulated larval growth whereas midday disturbances stimulated metamorphosis. The time when the daily disturbances caused fattening changed during metamorphosis so that younger tadpoles fattened when disturbed early in the photoperiod and older tadpoles fattened when disturbed at midday."}
{"id": "PMID:1087335", "title": "Thymus dependence of rabies vaccine.", "content": "Immune responses to rabies vaccine were compared with those to known thymus-dependent and thymus-independent antigens, in nude mice and their normal litter mates. No antibody response to, or protection against, challenge was observed in nude mice inoculated with either 1 or 2 doses of rabies vaccine. A single dose of the same vaccine induced a substantial antibody response and protected normal mice. These data indicate that the antigens in rabies vaccine that induce neutralizing antibody and protect mice are thymus-dependent.", "contents": "Thymus dependence of rabies vaccine. Immune responses to rabies vaccine were compared with those to known thymus-dependent and thymus-independent antigens, in nude mice and their normal litter mates. No antibody response to, or protection against, challenge was observed in nude mice inoculated with either 1 or 2 doses of rabies vaccine. A single dose of the same vaccine induced a substantial antibody response and protected normal mice. These data indicate that the antigens in rabies vaccine that induce neutralizing antibody and protect mice are thymus-dependent."}
{"id": "PMID:1087337", "title": "Precision of reinnervation of original postsynaptic sites in frog muscle after a nerve crush.", "content": "Regenerating neuromuscular junctions in the cutaneous pectoris muscle of the frog were examined by light and electron microscopy up to three months after crushing the motor nerve. The aim was to determine the precision of reinnervation of the original synaptic sites. More than 95% of the original postsynaptic membrane is recovered by nerve terminals and little, if any, synaptic contact is made on other portions of the muscle fibre surface. Even after prolonged denervation when the Schwann cells have retracted from 70-80% of the postsynaptic membrane, regenerating terminals return to and cover a large fraction of it. Although synapses are confined to the original synaptic sites, the pattern of innervation of muscle fibres is altered in several ways: (a) regenerating axon terminals can fail to branch leaving small stretches of postsynaptic membrane uncovered; (b) two terminal branches can lie side by side over a stretch of postsynaptic membrane normally occupied by one terminal; and (c) after growing along a stretch of postsynaptic membrane on one muscle fibre, terminals can leave it to end either in extracellular space or on the postsynaptic membrane of another fibre. Altogether the results demonstrate a strong and specific affinity between the original synaptic sites and regenerating nerve terminals.", "contents": "Precision of reinnervation of original postsynaptic sites in frog muscle after a nerve crush. Regenerating neuromuscular junctions in the cutaneous pectoris muscle of the frog were examined by light and electron microscopy up to three months after crushing the motor nerve. The aim was to determine the precision of reinnervation of the original synaptic sites. More than 95% of the original postsynaptic membrane is recovered by nerve terminals and little, if any, synaptic contact is made on other portions of the muscle fibre surface. Even after prolonged denervation when the Schwann cells have retracted from 70-80% of the postsynaptic membrane, regenerating terminals return to and cover a large fraction of it. Although synapses are confined to the original synaptic sites, the pattern of innervation of muscle fibres is altered in several ways: (a) regenerating axon terminals can fail to branch leaving small stretches of postsynaptic membrane uncovered; (b) two terminal branches can lie side by side over a stretch of postsynaptic membrane normally occupied by one terminal; and (c) after growing along a stretch of postsynaptic membrane on one muscle fibre, terminals can leave it to end either in extracellular space or on the postsynaptic membrane of another fibre. Altogether the results demonstrate a strong and specific affinity between the original synaptic sites and regenerating nerve terminals."}
{"id": "PMID:1087338", "title": "Altered patterns of innervation in frog muscle after denervation.", "content": "The pattern of reinnervation of muscle fibres after a nerve crush was examined in the cutaneous pectoris muscle of the frog by microscopy and electrophysiology. Normally, about 16% of the muscle fibres are innervated by more than one motor neuron. Two months after reinnervation, about 50% of the fibres are polyneuronally innervated and this high incidence persists for at least seven months. The total number of neurons reinnervating the muscle, as well as the number of muscle fibres comprising the muscle, are normal. However, nerve fibres sprout branches at the site of the crush, and, therefore, the number of axons entering the muscle is greater than normal. Regenerating axons contact muscle fibres precisely at the original synaptic sites and the terminal branches from different axons that end on the same muscle fibre often run side by side occupying stretches or original postsynaptic membrane normally covered by one terminal. Our findings indicate that the amount of synaptic contact during regeneration is limited by the amount of original postsynaptic membrane and that any number of axons that reach vacant portions of original postsynaptic membrane can make synaptic contact with it. Surprisingly, control cutaneous pectoris muscles, situated contralaterally to those that were denervated, also exhibited an abnormal pattern of innervation. Although neither nerve nor muscle was disturbed by the operation, there was a higher incidence of polyneuronal innervation (27% vs 16%) than in muscles of normal animals.", "contents": "Altered patterns of innervation in frog muscle after denervation. The pattern of reinnervation of muscle fibres after a nerve crush was examined in the cutaneous pectoris muscle of the frog by microscopy and electrophysiology. Normally, about 16% of the muscle fibres are innervated by more than one motor neuron. Two months after reinnervation, about 50% of the fibres are polyneuronally innervated and this high incidence persists for at least seven months. The total number of neurons reinnervating the muscle, as well as the number of muscle fibres comprising the muscle, are normal. However, nerve fibres sprout branches at the site of the crush, and, therefore, the number of axons entering the muscle is greater than normal. Regenerating axons contact muscle fibres precisely at the original synaptic sites and the terminal branches from different axons that end on the same muscle fibre often run side by side occupying stretches or original postsynaptic membrane normally covered by one terminal. Our findings indicate that the amount of synaptic contact during regeneration is limited by the amount of original postsynaptic membrane and that any number of axons that reach vacant portions of original postsynaptic membrane can make synaptic contact with it. Surprisingly, control cutaneous pectoris muscles, situated contralaterally to those that were denervated, also exhibited an abnormal pattern of innervation. Although neither nerve nor muscle was disturbed by the operation, there was a higher incidence of polyneuronal innervation (27% vs 16%) than in muscles of normal animals."}
{"id": "PMID:1087339", "title": "Intramembranous particle distribution at the node of Ranvier and adjacent axolemma in myelinated axons of the frog brain.", "content": "The plasma membrane of myelinated axons in the frog brain has been examined by the freeze-fracture technique. The cytoplasmic leaflet of the axolemma contains numerous randomly distributed particles in nodal and internodal regions but relatively fewer particles in the axoglial junctional portion of the paranodal region. Particle distribution is even less uniform in the outer leaflet of the axolemma, which contains a low concentration of particles in the internodal region and a relatively high concentration at the node of Ranvier (approximately 1200 particles mum-2). The nodal particles tend to be larger than most intramembranous particles, approaching 200 A diameter. The paranodal region of the leaflet is virtually devoid of such particles except in the narrow helical 'groove' which faces extracellular clefts between terminating glial processes. In places this pathway widens to form 'lakes' up to approximately 0.3 mum2 area which contain large numbers of large particles resembling those at the node. The concentration of particles at the node is in the same range as the concentration of sodium channels estimated to be in this region and it is suggested on the basis of their location and concentration that these particles represent ionophores. The distribution of particles in the paranodal region suggests that the large intramembranous particles do not have free access to the axoglial junctional portion of the membrane and therefore the movement of such particles along the paranodal region of the membrane may occur primarily in the membrane of the 'groove' spiraling through this portion of the axolemma. Such a restriction in surface area for particle movements on either side of the node of Ranvier could result in trapping of particles at the node and thus contribute to their concentration in the nodal axolemma.", "contents": "Intramembranous particle distribution at the node of Ranvier and adjacent axolemma in myelinated axons of the frog brain. The plasma membrane of myelinated axons in the frog brain has been examined by the freeze-fracture technique. The cytoplasmic leaflet of the axolemma contains numerous randomly distributed particles in nodal and internodal regions but relatively fewer particles in the axoglial junctional portion of the paranodal region. Particle distribution is even less uniform in the outer leaflet of the axolemma, which contains a low concentration of particles in the internodal region and a relatively high concentration at the node of Ranvier (approximately 1200 particles mum-2). The nodal particles tend to be larger than most intramembranous particles, approaching 200 A diameter. The paranodal region of the leaflet is virtually devoid of such particles except in the narrow helical 'groove' which faces extracellular clefts between terminating glial processes. In places this pathway widens to form 'lakes' up to approximately 0.3 mum2 area which contain large numbers of large particles resembling those at the node. The concentration of particles at the node is in the same range as the concentration of sodium channels estimated to be in this region and it is suggested on the basis of their location and concentration that these particles represent ionophores. The distribution of particles in the paranodal region suggests that the large intramembranous particles do not have free access to the axoglial junctional portion of the membrane and therefore the movement of such particles along the paranodal region of the membrane may occur primarily in the membrane of the 'groove' spiraling through this portion of the axolemma. Such a restriction in surface area for particle movements on either side of the node of Ranvier could result in trapping of particles at the node and thus contribute to their concentration in the nodal axolemma."}
{"id": "PMID:1087343", "title": "Nucleosides. 1. 9-(3'-Alkyl-3'-deoxy-beta-D-ribofuranosyl)adenines as lipophilic analogues of cordycepin. Synthesis and preliminary biological studies.", "content": "A series of lipophilic 9-(3'-alkyl-3'-deoxy-beta-D-ribofuranosyl)adenines of increasing chain length was synthesized from the corresponding branched sugars via titanium chloride catalyzed ribosylation of chloromercuri-6-benzamidopurine. Enhanced growth inhibitory activity was observed against (CCRF-CEM human lymphoblastic leukemia cells in culture as the length of the alkyl side chain in the sugar and the resultant lipophilic character of the nucleoside were increased. Experiments involving incorporation of radiolabeled uridine, thymidine, and leucine revealed that in contrast to cordycepin (1) the 3'-n-butyl and 3'-n-hexyl analogues 5 and 6 markedly inhibit not only RNA synthesis but DNA and protein synthesis as well.", "contents": "Nucleosides. 1. 9-(3'-Alkyl-3'-deoxy-beta-D-ribofuranosyl)adenines as lipophilic analogues of cordycepin. Synthesis and preliminary biological studies. A series of lipophilic 9-(3'-alkyl-3'-deoxy-beta-D-ribofuranosyl)adenines of increasing chain length was synthesized from the corresponding branched sugars via titanium chloride catalyzed ribosylation of chloromercuri-6-benzamidopurine. Enhanced growth inhibitory activity was observed against (CCRF-CEM human lymphoblastic leukemia cells in culture as the length of the alkyl side chain in the sugar and the resultant lipophilic character of the nucleoside were increased. Experiments involving incorporation of radiolabeled uridine, thymidine, and leucine revealed that in contrast to cordycepin (1) the 3'-n-butyl and 3'-n-hexyl analogues 5 and 6 markedly inhibit not only RNA synthesis but DNA and protein synthesis as well."}
{"id": "PMID:1087345", "title": "Influence of regenerative capacity and innervation on oncogenesis in the adult frog (Rana pipiens).", "content": "Twenty-two sarcomas were induced in 19 adult frogs (Rana pipiens) treated with 3-methylcholanthrene pellets. Thirteen of these tumors arose first in a denervated forelimb, and only 2 arose first in normal or nerve-supplemented control forelimbs (P = 0.004). The remaining tumors developed either as a second tumor in a tumor-bearing frog or in hindlimbs. The critical role of innervation in regenerative capacity suggests that the predilection to tumor formation in the denervated limbs may have resulted from their lessened regenerative capacity.", "contents": "Influence of regenerative capacity and innervation on oncogenesis in the adult frog (Rana pipiens). Twenty-two sarcomas were induced in 19 adult frogs (Rana pipiens) treated with 3-methylcholanthrene pellets. Thirteen of these tumors arose first in a denervated forelimb, and only 2 arose first in normal or nerve-supplemented control forelimbs (P = 0.004). The remaining tumors developed either as a second tumor in a tumor-bearing frog or in hindlimbs. The critical role of innervation in regenerative capacity suggests that the predilection to tumor formation in the denervated limbs may have resulted from their lessened regenerative capacity."}
{"id": "PMID:1087346", "title": "Effects of thymectomy and antithymocyte serum on spontaneous regression of Friend virus-induced erythroleukemia.", "content": "To evaluate the role of immune response in regression of leukemia, we studied the effect of immunosuppression on the spontaneous regression of a leukemia induced by a specific strain of Friend murine leukemia virus complex (RFV). Thymectomy of newborn but not adult outbred Swiss mice markedly inhibited regression. The effect of antithymocyte serum (ATS) on regression depended on the timing of ATS treatment. Regression was markedly inhibited in leukemic mice given ATS just before the start of regression. During leukemia development, ATS treatment but not thymectomy potentiated splenomegaly and delayed the start of regression. Both ATS treatment and neonatal thymectomy increased mortality as a function of the decrease in disease regression. Treatment with normal rabbit serum also inhibited regression but, when given during leukemia development, affected neither the splenomegalic response to RFV nor the number of deaths. The data demonstrated that an intact immune system was required for leukemia regression and suggested that some thymus-dependent parameter of immune response was a major factor in regression.", "contents": "Effects of thymectomy and antithymocyte serum on spontaneous regression of Friend virus-induced erythroleukemia. To evaluate the role of immune response in regression of leukemia, we studied the effect of immunosuppression on the spontaneous regression of a leukemia induced by a specific strain of Friend murine leukemia virus complex (RFV). Thymectomy of newborn but not adult outbred Swiss mice markedly inhibited regression. The effect of antithymocyte serum (ATS) on regression depended on the timing of ATS treatment. Regression was markedly inhibited in leukemic mice given ATS just before the start of regression. During leukemia development, ATS treatment but not thymectomy potentiated splenomegaly and delayed the start of regression. Both ATS treatment and neonatal thymectomy increased mortality as a function of the decrease in disease regression. Treatment with normal rabbit serum also inhibited regression but, when given during leukemia development, affected neither the splenomegalic response to RFV nor the number of deaths. The data demonstrated that an intact immune system was required for leukemia regression and suggested that some thymus-dependent parameter of immune response was a major factor in regression."}
{"id": "PMID:1087347", "title": "Suppressor factor secreted by T-lymphocytes from tumor-bearing mice.", "content": "Spleen cells from C57BL mice bearing the syngeneic carcinoma 3LL enhanced tumor growth. Tumor growth was also enhanced by a soluble factor found in the media of cultured spleen cells from tumor-bearing animals. This factor suppressed a protective immune response of the host and was found to be a product of T-lymphocytes. Removal of B-lymphocytes and macrophages did not prevent its appearance in the culture media, whereas removal of T-lymphocytes inhibited its appearance. Similar suppressor factors were obtained from C3H mice bearing the 3LL tumor and from mice with other tumors. The suppressing factor produced after the growth of 3LL tumor also enhanced the growth of other tumors. It could act on strains incompatible with the donor of the factor-producing cells. Hence tumor growth was possibly facilitated by soluble products of T-lymphocytes that were found in spleens of tumor-bearing mice and that nonspecifically suppressed immune defense mechanisms.", "contents": "Suppressor factor secreted by T-lymphocytes from tumor-bearing mice. Spleen cells from C57BL mice bearing the syngeneic carcinoma 3LL enhanced tumor growth. Tumor growth was also enhanced by a soluble factor found in the media of cultured spleen cells from tumor-bearing animals. This factor suppressed a protective immune response of the host and was found to be a product of T-lymphocytes. Removal of B-lymphocytes and macrophages did not prevent its appearance in the culture media, whereas removal of T-lymphocytes inhibited its appearance. Similar suppressor factors were obtained from C3H mice bearing the 3LL tumor and from mice with other tumors. The suppressing factor produced after the growth of 3LL tumor also enhanced the growth of other tumors. It could act on strains incompatible with the donor of the factor-producing cells. Hence tumor growth was possibly facilitated by soluble products of T-lymphocytes that were found in spleens of tumor-bearing mice and that nonspecifically suppressed immune defense mechanisms."}
{"id": "PMID:1087348", "title": "Cytotoxicity responses to melanoma cells by human lymphoid cell subpopulations.", "content": "Lymphoid cell subpopulations from normal donors and patients with malignant melanoma were assessed for cytotoxicity. Unfractionated mononuclear cells and T-cells from melanoma patients gave cell-mediated cytotoxic (CMC) responses to melanoma target cells but not to human fibroblasts. These specific CMC responses to melanoma cells were partially inhibited by autologous serum. Non-T-cells and nonrosetting cells from melanoma patients were not directly cytolytic for melanoma target cells; however these same subpopulations were cytotoxic in the presence of autologous serum, which indicated antibody-dependent cell cytotoxic responses. Non-T-cell subpopulations from normal donors were not cytotoxic when incubated with autologous serum. A third cytotoxic mechanism was demonstrated with complement (C3) receptor-activated lymphocytes. From both melanoma patients and normal donors, cells forming rosettes with erythrocyte-antibody-complement were nonspecifically cytotoxic for lung fibroblasts and melanoma target cells, These responses were independent of antibody, since melanoma serum presumably containing antibody to melanoma target cells neither enhanced nor blocked cytotoxicity mediated by C3 receptor-bearing lymphocytes. The results indicated that lymphoid cell subpopulations from the same melanoma patient could express at least three different lymphocyte-mediated cytotoxicity mechanisms against melanoma target cells.", "contents": "Cytotoxicity responses to melanoma cells by human lymphoid cell subpopulations. Lymphoid cell subpopulations from normal donors and patients with malignant melanoma were assessed for cytotoxicity. Unfractionated mononuclear cells and T-cells from melanoma patients gave cell-mediated cytotoxic (CMC) responses to melanoma target cells but not to human fibroblasts. These specific CMC responses to melanoma cells were partially inhibited by autologous serum. Non-T-cells and nonrosetting cells from melanoma patients were not directly cytolytic for melanoma target cells; however these same subpopulations were cytotoxic in the presence of autologous serum, which indicated antibody-dependent cell cytotoxic responses. Non-T-cell subpopulations from normal donors were not cytotoxic when incubated with autologous serum. A third cytotoxic mechanism was demonstrated with complement (C3) receptor-activated lymphocytes. From both melanoma patients and normal donors, cells forming rosettes with erythrocyte-antibody-complement were nonspecifically cytotoxic for lung fibroblasts and melanoma target cells, These responses were independent of antibody, since melanoma serum presumably containing antibody to melanoma target cells neither enhanced nor blocked cytotoxicity mediated by C3 receptor-bearing lymphocytes. The results indicated that lymphoid cell subpopulations from the same melanoma patient could express at least three different lymphocyte-mediated cytotoxicity mechanisms against melanoma target cells."}
{"id": "PMID:1087349", "title": "The Fv-2r resistance gene in mice: its effect on spleen colony formation by Friend virus-transformed cells.", "content": "To determine the mechanism by which the recessive Fv-2r gene confers complete resistance to spleen focus formation and to the induction of early splenomegaly characteristic of the Friend virus (FV) disease syndrome, we compared the parameters of FV infection in susceptible DBA/2 (D2) (Fv-1n, Fv-2s) and partially congenic D2.Fv-2r (Fv-1n, Fv-2r) mice. After infecting these mice with N-tropic FV complex, we followed the replication of spleen focus-forming virus (SFFV) and the generation of SFFV-transformed tumor colony-forming cells (CFC) in their spleens. By both parameters D2.Fv-2r mice were 20- to 100-fold less susceptible than DBA/2 controls, but the inhibition was only partial. Transplantation of washed spleen cells from SFFV-infected DBA/2 mice resulted in equal growth and recovery of tumor CFC from both D2.Fv-2r and Fv-2s mice. However, these tumor cells grew as colonies in Fv-2s mice, whereas they grew diffusely in the spleens of D2.Fv-2r hosts and did not develop into macroscopically or microscopically visible colonies. Thus the completeness of the resistance to spleen focus formation that defines the Fv-2r gene was reflected only in the complete suppression of the colonial growth of tumor cells, whereas the other parameters of infection showed no or only partial inhibition.", "contents": "The Fv-2r resistance gene in mice: its effect on spleen colony formation by Friend virus-transformed cells. To determine the mechanism by which the recessive Fv-2r gene confers complete resistance to spleen focus formation and to the induction of early splenomegaly characteristic of the Friend virus (FV) disease syndrome, we compared the parameters of FV infection in susceptible DBA/2 (D2) (Fv-1n, Fv-2s) and partially congenic D2.Fv-2r (Fv-1n, Fv-2r) mice. After infecting these mice with N-tropic FV complex, we followed the replication of spleen focus-forming virus (SFFV) and the generation of SFFV-transformed tumor colony-forming cells (CFC) in their spleens. By both parameters D2.Fv-2r mice were 20- to 100-fold less susceptible than DBA/2 controls, but the inhibition was only partial. Transplantation of washed spleen cells from SFFV-infected DBA/2 mice resulted in equal growth and recovery of tumor CFC from both D2.Fv-2r and Fv-2s mice. However, these tumor cells grew as colonies in Fv-2s mice, whereas they grew diffusely in the spleens of D2.Fv-2r hosts and did not develop into macroscopically or microscopically visible colonies. Thus the completeness of the resistance to spleen focus formation that defines the Fv-2r gene was reflected only in the complete suppression of the colonial growth of tumor cells, whereas the other parameters of infection showed no or only partial inhibition."}
{"id": "PMID:1087350", "title": "Evidence for rhythms of mitotic activity in normal and adenocarcinoma cells of the renal tubules of Rana pipiens.", "content": "Kidneys were studied from 1,111 leopard frogs (Rana pipiens) after exposures to a 12-hour light-12-hour dark environment. Circadian mitotic rhythms were observed in cells of normal and spontaneous adenocarcinomatous proximal convoluted tubules from these animals. Student's t-tests verified the existence of two distinct cell populations, normal and adenocarcinomatous, in the proximal tubules. Both the mitotic variations and the t-test values were significant with P values of 0.05 or less. Mean mitotic values for the normal and spontaneous adenocarcinomatous proximal tubule epithelia were generally lower during the light-phase and higher during the dark-phase periods of the control day. The mitotic rhythm of the cancerous epithelium was almost always superimposable in outline over that of the normal epithelium during the same experimental period; i.e., the correspondence of phases of the normal and cancerous mitoses was almost identical in a single graph, although the amplitude of the cancerous mitoses was always higher. In addition to the reproducible circadian rhythms, the data demonstrated evidence for the existence of ultradian, as well as circannual rhythms.", "contents": "Evidence for rhythms of mitotic activity in normal and adenocarcinoma cells of the renal tubules of Rana pipiens. Kidneys were studied from 1,111 leopard frogs (Rana pipiens) after exposures to a 12-hour light-12-hour dark environment. Circadian mitotic rhythms were observed in cells of normal and spontaneous adenocarcinomatous proximal convoluted tubules from these animals. Student's t-tests verified the existence of two distinct cell populations, normal and adenocarcinomatous, in the proximal tubules. Both the mitotic variations and the t-test values were significant with P values of 0.05 or less. Mean mitotic values for the normal and spontaneous adenocarcinomatous proximal tubule epithelia were generally lower during the light-phase and higher during the dark-phase periods of the control day. The mitotic rhythm of the cancerous epithelium was almost always superimposable in outline over that of the normal epithelium during the same experimental period; i.e., the correspondence of phases of the normal and cancerous mitoses was almost identical in a single graph, although the amplitude of the cancerous mitoses was always higher. In addition to the reproducible circadian rhythms, the data demonstrated evidence for the existence of ultradian, as well as circannual rhythms."}
{"id": "PMID:1087351", "title": "An assessment of intratumor phagocytic and surface marker-bearing cells in a series of autochthonous and early passaged chemically induced murine sarcomas.", "content": "Single cell suspensions from five different 3-methylcholanthrene-induced tumors in CBA mice were examined in the autochthonous host and sequentially for 5-11 passages. They were also examined for Fc receptor-bearing, phagocytic, theta antigen-positive, and surface immunoglobulin-bearing cells. The preparations contained a high proportion of phagocytic and marker-bearing cells both in the original host and during early passage. This proportion was consistent for any particular tumor and passage. Between different tumors, however, the proportions were sufficiently different to allow the tumor to be identified on this basis; this suggested that various chemically induced tumors may be unique in their tumor-host relationship as measured by the type of cells which infiltrate them. With on-going early passage of the tumors, the proportion of marker-bearing cells decreased to a constant level in most instances, mainly because of a reduction in the percentage of phagocytic cells. The tumor with the least macrophages (MBQA, less than 5%) consistently appeared more rapidly and killed the host more rapidly than did the tumor with the most macrophages (MBQD, 15-30%), but was not significantly different in its growth rate. The theta antigen- and Fc receptor-positive cells within these tumors were derived from the animal receiving the tumor inoculum, and thus represented host cell infiltration of the tumor. The results were discussed with reference to fundamental concepts of the immunology of chemically induced tumors and the importance of host cell infiltration within these tumors.", "contents": "An assessment of intratumor phagocytic and surface marker-bearing cells in a series of autochthonous and early passaged chemically induced murine sarcomas. Single cell suspensions from five different 3-methylcholanthrene-induced tumors in CBA mice were examined in the autochthonous host and sequentially for 5-11 passages. They were also examined for Fc receptor-bearing, phagocytic, theta antigen-positive, and surface immunoglobulin-bearing cells. The preparations contained a high proportion of phagocytic and marker-bearing cells both in the original host and during early passage. This proportion was consistent for any particular tumor and passage. Between different tumors, however, the proportions were sufficiently different to allow the tumor to be identified on this basis; this suggested that various chemically induced tumors may be unique in their tumor-host relationship as measured by the type of cells which infiltrate them. With on-going early passage of the tumors, the proportion of marker-bearing cells decreased to a constant level in most instances, mainly because of a reduction in the percentage of phagocytic cells. The tumor with the least macrophages (MBQA, less than 5%) consistently appeared more rapidly and killed the host more rapidly than did the tumor with the most macrophages (MBQD, 15-30%), but was not significantly different in its growth rate. The theta antigen- and Fc receptor-positive cells within these tumors were derived from the animal receiving the tumor inoculum, and thus represented host cell infiltration of the tumor. The results were discussed with reference to fundamental concepts of the immunology of chemically induced tumors and the importance of host cell infiltration within these tumors."}
{"id": "PMID:1087352", "title": "Dependency of sister chromatid exchange in T- and B-cells on the incorporation of deoxyribonucleosides into chromosomal DNA.", "content": "Human T-cells (CCRF-HSB2) did not incorporate tritiated thymidine ([3H]TDR)--1.0-5.0 muCi/ml--into the nuclei, where.as they readily incorporated tritiated deoxycytidine (E13H]CDR). When contamination with pleuropneumonia-like organisms was ruled out, these findings strongly suggested a deficiency of the enzyme thymidine kinase in the cells. Human B-cells (CCRF-SB) and normal T-lymphocytes (NTL) readily incorporated [3H]CDR, [3H]TDR, and tritiated 5-bromodeoxyuridine, and they clearly exhibited differential staining of the sister chromatids (SCD). When nonisotopic bromodeoxyuridine (BUDR), 10(-6)-10(-4) M, was used with the B-cells and NTL, SCD were clearly evident and sister chromatid exchange (SCE) was relatively infrequent; when the concentration was 10(-7) M, SCD staining was poor but the frequency of SCE was high. SCE frequencies in NTL, measured by autoradiography after incorporation of [3H]CDR, were the same as SCE frequencies measured by staining with BUDR at 10(-4) M. In the case of CCRF-HSB2, 10(-4) M BUDR produced relatively high frequencies of SCE as did 10(-7) M BUDR with the former two cells. However, [3H]CDR with CCRF-HSB2 gave relatively low frequencies of SCE, of the magnitude observed after 10(-4) M BUDR was used with NTL and the B-cells. Thus the high frequency of SCE in CCRF-HSB2 cells may have been due to the staining property of chromosomes that had incorporated low levels of BUDR.", "contents": "Dependency of sister chromatid exchange in T- and B-cells on the incorporation of deoxyribonucleosides into chromosomal DNA. Human T-cells (CCRF-HSB2) did not incorporate tritiated thymidine ([3H]TDR)--1.0-5.0 muCi/ml--into the nuclei, where.as they readily incorporated tritiated deoxycytidine (E13H]CDR). When contamination with pleuropneumonia-like organisms was ruled out, these findings strongly suggested a deficiency of the enzyme thymidine kinase in the cells. Human B-cells (CCRF-SB) and normal T-lymphocytes (NTL) readily incorporated [3H]CDR, [3H]TDR, and tritiated 5-bromodeoxyuridine, and they clearly exhibited differential staining of the sister chromatids (SCD). When nonisotopic bromodeoxyuridine (BUDR), 10(-6)-10(-4) M, was used with the B-cells and NTL, SCD were clearly evident and sister chromatid exchange (SCE) was relatively infrequent; when the concentration was 10(-7) M, SCD staining was poor but the frequency of SCE was high. SCE frequencies in NTL, measured by autoradiography after incorporation of [3H]CDR, were the same as SCE frequencies measured by staining with BUDR at 10(-4) M. In the case of CCRF-HSB2, 10(-4) M BUDR produced relatively high frequencies of SCE as did 10(-7) M BUDR with the former two cells. However, [3H]CDR with CCRF-HSB2 gave relatively low frequencies of SCE, of the magnitude observed after 10(-4) M BUDR was used with NTL and the B-cells. Thus the high frequency of SCE in CCRF-HSB2 cells may have been due to the staining property of chromosomes that had incorporated low levels of BUDR."}
{"id": "PMID:1087353", "title": "Evidence for splenic suppressor cells in C3H/He, T-cell-deprived C3H/He, and nude mice bearing a 3-methylcholanthrene-induced fibrosarcoma.", "content": "Suppressor cells were demonstrated in the spleens of C3H/He mice carrying 3-methylcholantrene-induced fibrosarcomas. These cells inhibited the in vitro reactivity of normal lymphocytes to T- and B-cell mitogens. They disappeared within a few days after the tumor was surgically removed. Pretreatment of spleen cells (ScC) from tumor-bearing (TB) mice with either iron and a nagnet, antiserum against Thy 1.2 antigen plus complement, or antiserum against immunoglobulin plus complement demonstrated that the suppressor cells were adherent, non-T-cells bearing immunoglobulin at their surfaces. The suppressive effect could still be demonstrated by addition of SpC from TB mice 24 or 48 hours after phytohemagglutinin stimulation of normal SpC, SpC from TB C3H/He mice inhibited mitogen-induced stimulation of both C3H/He and DBA/2 lymphocytes. In T-cell-deprived TB C3H/He mice, suppressor cells were also observed and had the same characteristics as those in non-T-cell-deprived mice. In nude mice, however, although suppressor cells were active, they were not adherent and did not bear immunoglobulin at their surfaces. The existence of these suppressor cells may be one reason why the immune system of TB animals is unable to reject the tumor.", "contents": "Evidence for splenic suppressor cells in C3H/He, T-cell-deprived C3H/He, and nude mice bearing a 3-methylcholanthrene-induced fibrosarcoma. Suppressor cells were demonstrated in the spleens of C3H/He mice carrying 3-methylcholantrene-induced fibrosarcomas. These cells inhibited the in vitro reactivity of normal lymphocytes to T- and B-cell mitogens. They disappeared within a few days after the tumor was surgically removed. Pretreatment of spleen cells (ScC) from tumor-bearing (TB) mice with either iron and a nagnet, antiserum against Thy 1.2 antigen plus complement, or antiserum against immunoglobulin plus complement demonstrated that the suppressor cells were adherent, non-T-cells bearing immunoglobulin at their surfaces. The suppressive effect could still be demonstrated by addition of SpC from TB mice 24 or 48 hours after phytohemagglutinin stimulation of normal SpC, SpC from TB C3H/He mice inhibited mitogen-induced stimulation of both C3H/He and DBA/2 lymphocytes. In T-cell-deprived TB C3H/He mice, suppressor cells were also observed and had the same characteristics as those in non-T-cell-deprived mice. In nude mice, however, although suppressor cells were active, they were not adherent and did not bear immunoglobulin at their surfaces. The existence of these suppressor cells may be one reason why the immune system of TB animals is unable to reject the tumor."}
{"id": "PMID:1087356", "title": "The effect of adrenaline on the K+ -activated hyperpolarization of the sympathetic ganglion cell membrane in bullfrogs.", "content": "Effects of adrenaline on K+ -activated hyperpolarization of bullfrog sympathetic ganglion cells, preganglionic nerve axons and splanchnic nerve axons were studied. The K+ -activated hyperpolarization recorded from ganglion-postgangaline (0.3 mM). On the other hand, the K+ -activated hyperpolarization of postganglionic, preganglionic nerve axons and splanchnic nerve axons was not affected under the same experimental conditions. The facil-tatory effect of adrenaline on the K+ -activated hyperpolization was gradually intensified when the application of adrenaline was sustained for up to 60 min. The effect was reversible. The K+ -activated hyperpolarization recorded from ganglion cell-bodies by intracellular microelectrodes was similarly augmented by adrenaline (0.1 mM). Adrenaline in this concentration, however, caused no significant changes of the membrane potential and conductance in the presence of ouabain (0.002 mM) which completely and reversibly inhibited K+-activated hyperpolarization. Noradrenaline showed similar but less effected and isoproterenol showed no significant effect on the K+ -activated hyperpolarization. The facilitatory effect of adrenaline was inhibited in the presence of phentolamine but not of DCI. These results suggested that the intraganglionic membrane of ganglion cells possessed some kind of adrenergic alpha-receptors and the electrogenic Na+ pump of ganglion cells was accelerated by some unknown mechanism when adrenaline reacted to these receptors.", "contents": "The effect of adrenaline on the K+ -activated hyperpolarization of the sympathetic ganglion cell membrane in bullfrogs. Effects of adrenaline on K+ -activated hyperpolarization of bullfrog sympathetic ganglion cells, preganglionic nerve axons and splanchnic nerve axons were studied. The K+ -activated hyperpolarization recorded from ganglion-postgangaline (0.3 mM). On the other hand, the K+ -activated hyperpolarization of postganglionic, preganglionic nerve axons and splanchnic nerve axons was not affected under the same experimental conditions. The facil-tatory effect of adrenaline on the K+ -activated hyperpolization was gradually intensified when the application of adrenaline was sustained for up to 60 min. The effect was reversible. The K+ -activated hyperpolarization recorded from ganglion cell-bodies by intracellular microelectrodes was similarly augmented by adrenaline (0.1 mM). Adrenaline in this concentration, however, caused no significant changes of the membrane potential and conductance in the presence of ouabain (0.002 mM) which completely and reversibly inhibited K+-activated hyperpolarization. Noradrenaline showed similar but less effected and isoproterenol showed no significant effect on the K+ -activated hyperpolarization. The facilitatory effect of adrenaline was inhibited in the presence of phentolamine but not of DCI. These results suggested that the intraganglionic membrane of ganglion cells possessed some kind of adrenergic alpha-receptors and the electrogenic Na+ pump of ganglion cells was accelerated by some unknown mechanism when adrenaline reacted to these receptors."}
{"id": "PMID:1087357", "title": "Effect of 1-(m-chlorophenyl)-3-N,N-dimethyl-carbamoyl-5-methoxypyrazole (PZ-177) on drug-metabolizing enzyme on rat liver.", "content": "Effect of 1-(m-chlorphenyl)-3-N,N-dimethylcarbamoyl-5-methoxypyrazole (PZ-177) (62.5 and 250 mg/kg) on rat liver was investigated by measuring liver weight and drug-metabolizing enzyme activity. The effects of PZ-177 were compared with those of phenobarbital, phenylbutazone, and tiaramide hydrochloride. Increase of liver weight and liver/body weight ratio was observed in the rats treated with PZ-177 or phenobarbital, however, normal values were reverted to 1--2 weeks after treatment. PZ-177 similar to phenobarbital, significantly enhanced the activity of aminopyrine demethylase and aniline hydroxylase after 1,2, and 4 weeks of treatment. In contrast, tiaramide hydrochloride decreased the activity of aminopyrine demethylase and aniline hydroxylase after 1 week of treatment, and significantly enhanced the activity of these enzymes after 4 weeks. The content of cytochrome P-450 and the activity of NADPH cytochrome C reductase were also increased by treatment with PZ-177. The sleeping time by hexobarbital was shortened significantly by the administration of PZ-177. Vmax for both aminopyrine demethylase and aniline hydroxylase increased by treatment with PZ-177. However, only the Km for aniline hydroxylase was increased by treatment with PZ-177. From the results of these experiments, PZ-177 may be classified as a phenobarbital-type inducer.", "contents": "Effect of 1-(m-chlorophenyl)-3-N,N-dimethyl-carbamoyl-5-methoxypyrazole (PZ-177) on drug-metabolizing enzyme on rat liver. Effect of 1-(m-chlorphenyl)-3-N,N-dimethylcarbamoyl-5-methoxypyrazole (PZ-177) (62.5 and 250 mg/kg) on rat liver was investigated by measuring liver weight and drug-metabolizing enzyme activity. The effects of PZ-177 were compared with those of phenobarbital, phenylbutazone, and tiaramide hydrochloride. Increase of liver weight and liver/body weight ratio was observed in the rats treated with PZ-177 or phenobarbital, however, normal values were reverted to 1--2 weeks after treatment. PZ-177 similar to phenobarbital, significantly enhanced the activity of aminopyrine demethylase and aniline hydroxylase after 1,2, and 4 weeks of treatment. In contrast, tiaramide hydrochloride decreased the activity of aminopyrine demethylase and aniline hydroxylase after 1 week of treatment, and significantly enhanced the activity of these enzymes after 4 weeks. The content of cytochrome P-450 and the activity of NADPH cytochrome C reductase were also increased by treatment with PZ-177. The sleeping time by hexobarbital was shortened significantly by the administration of PZ-177. Vmax for both aminopyrine demethylase and aniline hydroxylase increased by treatment with PZ-177. However, only the Km for aniline hydroxylase was increased by treatment with PZ-177. From the results of these experiments, PZ-177 may be classified as a phenobarbital-type inducer."}
{"id": "PMID:1087359", "title": "[Pathology of exogenous mycotic endophthalmitis (author's transl)].", "content": "The histological examination of five bulbi with exogenous mycotic endophthalmitis reveals invasion of fungi confined to the anterior segment including the anterior vitreous body. Hyphae are found within abscesses, and granulomas respectively. The posterior eye remains relatively free, however a posterior vitreous detachment always exists together with a massive retraction of the vitreous gel. These findings should be kept in mind if intravitreal injections of amphotericin-B, or a vitrectomy are considered. After intravitreal injections of amphotericin-B no retinal lesions could be revealed.", "contents": "[Pathology of exogenous mycotic endophthalmitis (author's transl)]. The histological examination of five bulbi with exogenous mycotic endophthalmitis reveals invasion of fungi confined to the anterior segment including the anterior vitreous body. Hyphae are found within abscesses, and granulomas respectively. The posterior eye remains relatively free, however a posterior vitreous detachment always exists together with a massive retraction of the vitreous gel. These findings should be kept in mind if intravitreal injections of amphotericin-B, or a vitrectomy are considered. After intravitreal injections of amphotericin-B no retinal lesions could be revealed."}
{"id": "PMID:1087360", "title": "[Observations on the pathogenesis of essential progressive iris atrophy with corneal dystrophy and secondary glaucoma (author's transl)].", "content": "A report is given of a case of a unilateral condition of this type in a 30-year-old woman, who was observed and repeatedly photographed for more as 7 years.", "contents": "[Observations on the pathogenesis of essential progressive iris atrophy with corneal dystrophy and secondary glaucoma (author's transl)]. A report is given of a case of a unilateral condition of this type in a 30-year-old woman, who was observed and repeatedly photographed for more as 7 years."}
{"id": "PMID:1087361", "title": "[Indications and limits of local tri-methacycline therapy in pyogenic infections of the eye (author's transl)].", "content": "In addition to the preoperative treatment of acute dacryocystitis local administration of tri-methacycline proved to be of benefit in cases of endophthalmitis consequent to injury. Its subconjunctival administration is the therapy of choice for serpentic ulcers of the cornea.", "contents": "[Indications and limits of local tri-methacycline therapy in pyogenic infections of the eye (author's transl)]. In addition to the preoperative treatment of acute dacryocystitis local administration of tri-methacycline proved to be of benefit in cases of endophthalmitis consequent to injury. Its subconjunctival administration is the therapy of choice for serpentic ulcers of the cornea."}
{"id": "PMID:1087366", "title": "Immunospecific labeling of mouse lymphocytes in the scanning electron microscope.", "content": "Bone marrow-derived (B) and thymus-derived (T) Balb/c mouse lymphocytes were identified in the scanning electron microscope (SEM) by the immunospecific attachment of one of several kinds of large-molecular-weight markers distinguishable in SEM. These markers (tobacco mosaic virus, keyhole limpet hemocyanin, bushy stunt virus, and bacteriophage T4) could be modified with hapten groups and linked with anti-hapten antibody, in an indirect (sandwich) scheme, to hapten-modified anti-cell-surface antibody bound to the cell surface. Hapten-modified antibodies to B cell antigens (goat anti-mouse-immunoglobulin) or to T cell antigens (rabbit anti-mouse brain) were employed to identify these two lymphoid cell types in unfractionated spleen, mesenteric lymph node, bone marrow, and thymus cell populations. The topography of B cells was always indistinguishable from that of T cells. No surface features were found to be unique to either cell type. In suspension, the majority of B and T cells had one or no microvilli regardless of the tissue source of the labeled cells. Cells in suspension that had microvilli (usually 10% of the total cell population) were always unlabeled. However, after cell contact with a glass surface, approximately half of both the B and T cell population had a villous topography.", "contents": "Immunospecific labeling of mouse lymphocytes in the scanning electron microscope. Bone marrow-derived (B) and thymus-derived (T) Balb/c mouse lymphocytes were identified in the scanning electron microscope (SEM) by the immunospecific attachment of one of several kinds of large-molecular-weight markers distinguishable in SEM. These markers (tobacco mosaic virus, keyhole limpet hemocyanin, bushy stunt virus, and bacteriophage T4) could be modified with hapten groups and linked with anti-hapten antibody, in an indirect (sandwich) scheme, to hapten-modified anti-cell-surface antibody bound to the cell surface. Hapten-modified antibodies to B cell antigens (goat anti-mouse-immunoglobulin) or to T cell antigens (rabbit anti-mouse brain) were employed to identify these two lymphoid cell types in unfractionated spleen, mesenteric lymph node, bone marrow, and thymus cell populations. The topography of B cells was always indistinguishable from that of T cells. No surface features were found to be unique to either cell type. In suspension, the majority of B and T cells had one or no microvilli regardless of the tissue source of the labeled cells. Cells in suspension that had microvilli (usually 10% of the total cell population) were always unlabeled. However, after cell contact with a glass surface, approximately half of both the B and T cell population had a villous topography."}
{"id": "PMID:1087374", "title": "Effect of p-nitrophenyl diazonium fluoroborate on cholinergic mechanisms.", "content": "Electrophysiological experiments were done to investigate the effect o p-nitrophenyl diazonium fluoroborate (p-NPD) on motor endplates of the frog's m. cutaneus pectoris. The compound has no direct depolarizing effect on the postsynaptic membrane and stabilizes it irreversibly when added to the bath. Longtime iontophoretical applications of p-NPD produce a biphasic effect: initially a potentiation of the depolarizations due to acetylcholine (ACh) (both iontophoretically applied and presynaptically liberated), and subsequently an inhibition of the response to ACh. When the acetylcholinesterase (AChE) is inactivated previously, only the inhibiting effect of the compound is demonstrable. The association constant of p-NPD to purified AChE and to membrane fragments of electroplax was determined by biochemical methods. The compound's affinity to the AChE was found to be about 20 times greater than to the acetylcholine receptor (AChR). Iontophoretical application of p-NPD to cholinergic neurons in the hippocampal cortex of the cat also produced the characteristic biphasic effect on ACh-induced activity of these investigated neurons. The results suggest that the biphasic effect depends on the capacity of p-NPD to combine with both the AChE and the AChR. The AChE is first inhibited with low concentrations thereby potentiating the ACh response. At higher concentrations the AChR's are progressively inhibited too, thereby diminishing the excitability of the postsynaptic membrane up to a complete block.", "contents": "Effect of p-nitrophenyl diazonium fluoroborate on cholinergic mechanisms. Electrophysiological experiments were done to investigate the effect o p-nitrophenyl diazonium fluoroborate (p-NPD) on motor endplates of the frog's m. cutaneus pectoris. The compound has no direct depolarizing effect on the postsynaptic membrane and stabilizes it irreversibly when added to the bath. Longtime iontophoretical applications of p-NPD produce a biphasic effect: initially a potentiation of the depolarizations due to acetylcholine (ACh) (both iontophoretically applied and presynaptically liberated), and subsequently an inhibition of the response to ACh. When the acetylcholinesterase (AChE) is inactivated previously, only the inhibiting effect of the compound is demonstrable. The association constant of p-NPD to purified AChE and to membrane fragments of electroplax was determined by biochemical methods. The compound's affinity to the AChE was found to be about 20 times greater than to the acetylcholine receptor (AChR). Iontophoretical application of p-NPD to cholinergic neurons in the hippocampal cortex of the cat also produced the characteristic biphasic effect on ACh-induced activity of these investigated neurons. The results suggest that the biphasic effect depends on the capacity of p-NPD to combine with both the AChE and the AChR. The AChE is first inhibited with low concentrations thereby potentiating the ACh response. At higher concentrations the AChR's are progressively inhibited too, thereby diminishing the excitability of the postsynaptic membrane up to a complete block."}
{"id": "PMID:1087375", "title": "Effect of atropine and oxotremorine on the release of acetylcholine from the frog spinal cord.", "content": "The in vitro frog spinal cord has been used to study acetylcholine (ACh) release and spinal root potentials. The preparation bathed in an eserine-containing medium spontaneously released ACh into the bathing fluid. This release was enhanced by atropine in a dose-related and long-lasting manner and transiently by oxotremorine. The release rate of ACh was increased by low frequency ventral root stimulation; this increase was found to be proportionally the same after application of atropine. Oxotremorine did not modify the elctrically-evoked ACh release but blocked or reduced the effect of atropine. It is concluded that the stimulatory action of atropine on ACh output cannot be entirely explained by an interaction of atropine with presynaptic cholinergic receptors and that other indirect mechanisms (via interneurones) may play a role in this effect.", "contents": "Effect of atropine and oxotremorine on the release of acetylcholine from the frog spinal cord. The in vitro frog spinal cord has been used to study acetylcholine (ACh) release and spinal root potentials. The preparation bathed in an eserine-containing medium spontaneously released ACh into the bathing fluid. This release was enhanced by atropine in a dose-related and long-lasting manner and transiently by oxotremorine. The release rate of ACh was increased by low frequency ventral root stimulation; this increase was found to be proportionally the same after application of atropine. Oxotremorine did not modify the elctrically-evoked ACh release but blocked or reduced the effect of atropine. It is concluded that the stimulatory action of atropine on ACh output cannot be entirely explained by an interaction of atropine with presynaptic cholinergic receptors and that other indirect mechanisms (via interneurones) may play a role in this effect."}
{"id": "PMID:1087377", "title": "Changes in human  lymphocyte subpopulation in Hodgkin's lymphoma.", "content": "An investigation of 33 patients with Hodgkin's lymphoma--both treated and untreated--has revealed lymphocytopenia and a significant decline in the absolute number of T lymphocytes labeled with rosettes with sheep erythrocytes to be due in these patients not to the tumorous disease itself, but probably to the intensive nature of the therapy. A marked decline in the absolute number of B lymphocyte subpopulation, determined by the formation of rosettes with mouse erythrocytes, occurs during a fresh relapse of patients with an active Hodgkin's lymphoma. This decline is probably independent of the preceding treatment, of the clinical stage of the disease and of its histological type.", "contents": "Changes in human  lymphocyte subpopulation in Hodgkin's lymphoma. An investigation of 33 patients with Hodgkin's lymphoma--both treated and untreated--has revealed lymphocytopenia and a significant decline in the absolute number of T lymphocytes labeled with rosettes with sheep erythrocytes to be due in these patients not to the tumorous disease itself, but probably to the intensive nature of the therapy. A marked decline in the absolute number of B lymphocyte subpopulation, determined by the formation of rosettes with mouse erythrocytes, occurs during a fresh relapse of patients with an active Hodgkin's lymphoma. This decline is probably independent of the preceding treatment, of the clinical stage of the disease and of its histological type."}
{"id": "PMID:1087378", "title": "T and B lymphocytes in malignant melanoma patients.", "content": "The method of formation of spontaneous (E) and immune (EAC) rosettes and the test of lymphocyte survival in short-term cultures with phytohemagglutinin (PHA) have been used as parameters of cellular immunocompetence in 64 patients with malignant melanoma and 33 control donors. Significant changes have been found in the number of T lymphocytes whose values corresponded to the clinical stage as well as to the clinical postoperational course in the patients. A decreased number of T lymphocytes was a sign of progression of the disease, while a normal value indicated remission. Lowered ratios of T lymphocytes were followed by an increased proportion of both the B lymphocytes and the null, nonrosetting cell elements. The results in patients with malignant melanoma permit the relevant parameters to be considered as an effective in vitro correlate of cellular immunity of these patients.", "contents": "T and B lymphocytes in malignant melanoma patients. The method of formation of spontaneous (E) and immune (EAC) rosettes and the test of lymphocyte survival in short-term cultures with phytohemagglutinin (PHA) have been used as parameters of cellular immunocompetence in 64 patients with malignant melanoma and 33 control donors. Significant changes have been found in the number of T lymphocytes whose values corresponded to the clinical stage as well as to the clinical postoperational course in the patients. A decreased number of T lymphocytes was a sign of progression of the disease, while a normal value indicated remission. Lowered ratios of T lymphocytes were followed by an increased proportion of both the B lymphocytes and the null, nonrosetting cell elements. The results in patients with malignant melanoma permit the relevant parameters to be considered as an effective in vitro correlate of cellular immunity of these patients."}
{"id": "PMID:1087379", "title": "[Dimer-X ventriculography (author's transl)].", "content": "After the first report on ventriculography with a water-soluble contrast medium, Conray, by Campbell et al., many authors reported complications such as convulsion which are attributable to the irritating effect of this agent. On the other hand its value has been appriciated. Recently Gonsette recommended a new water-soluble contrast medium, meglumine iocarnate (Dimer-X), for ventriculography and reported that this new agent is less epileptogenic and less harmful. Because of these advantages, they have been performing ventriculography routinely with Dimer-X and recognized its usefulness. The only noticeable complication has been the transient elevation of body temperature in almost a half of the series. As far as our experience goes there is no case of convulsive seizure as complication. As compared to gas ventriculography, the fine details of dimension and configuration of the ventricles are far more beautifully delineated with the water-soluble contrast media.", "contents": "[Dimer-X ventriculography (author's transl)]. After the first report on ventriculography with a water-soluble contrast medium, Conray, by Campbell et al., many authors reported complications such as convulsion which are attributable to the irritating effect of this agent. On the other hand its value has been appriciated. Recently Gonsette recommended a new water-soluble contrast medium, meglumine iocarnate (Dimer-X), for ventriculography and reported that this new agent is less epileptogenic and less harmful. Because of these advantages, they have been performing ventriculography routinely with Dimer-X and recognized its usefulness. The only noticeable complication has been the transient elevation of body temperature in almost a half of the series. As far as our experience goes there is no case of convulsive seizure as complication. As compared to gas ventriculography, the fine details of dimension and configuration of the ventricles are far more beautifully delineated with the water-soluble contrast media."}
{"id": "PMID:1087380", "title": "The excessively small ventricle on computed axial tomography of the brain.", "content": "Computed axial tomography has made it possible to observe excessively small ventricles in a variety of disorders of the CNS. This finding is presumably due to non-specific and diffuse swelling of brain parenchyma, and is illustrated in cases of trauma, neoplasm, encephalitis, pseudotumor cerebri, metabolic disorder, and probable dilantin effect. It is likely that other causes will be encountered in the future.", "contents": "The excessively small ventricle on computed axial tomography of the brain. Computed axial tomography has made it possible to observe excessively small ventricles in a variety of disorders of the CNS. This finding is presumably due to non-specific and diffuse swelling of brain parenchyma, and is illustrated in cases of trauma, neoplasm, encephalitis, pseudotumor cerebri, metabolic disorder, and probable dilantin effect. It is likely that other causes will be encountered in the future."}
{"id": "PMID:1087383", "title": "The effect of L-5-hydroxytryptophan on self-mutilatin in Lesch-Nyhan disease: a negative report.", "content": "Four patients with Lesch-Nyhan disease were treated with L-5-hydroxytryptophan, alone, or in combination with carbidopa, and their effect on self-mutilatory behavior was compared to that observed during periods in which placebo was administered. Observation periods were carefully standardized because of the ease with which patient-observer interactions can influence the behavior of the Lesch-Nyhan patient. The dosage level was sufficient to produce undesirable side-effects (diarrhea and vomiting). No effect on self-mutilation was observed under test conditions in the hospital or in the natural environment of the home. These results contrast with previous beneficial effects reported by others.", "contents": "The effect of L-5-hydroxytryptophan on self-mutilatin in Lesch-Nyhan disease: a negative report. Four patients with Lesch-Nyhan disease were treated with L-5-hydroxytryptophan, alone, or in combination with carbidopa, and their effect on self-mutilatory behavior was compared to that observed during periods in which placebo was administered. Observation periods were carefully standardized because of the ease with which patient-observer interactions can influence the behavior of the Lesch-Nyhan patient. The dosage level was sufficient to produce undesirable side-effects (diarrhea and vomiting). No effect on self-mutilation was observed under test conditions in the hospital or in the natural environment of the home. These results contrast with previous beneficial effects reported by others."}
{"id": "PMID:1087388", "title": "[Indications and contraindications for surgery in coronary insufficiency].", "content": "The indications to aortocoronary by-pass technique have during the last two years developed thanks to surgical, emodinamic and clinical exsperiences. The mortality of patients in whom surgery is limited electively to the intervention of aorto-coronary by-pass is at present around 4%. The principal indications to surgery are: a) existence of more than one coronary artery disease; b) a good diameter of the by-passing artery (more than 1 mm) corresponding to the site of the by-pass graft; c) a left ventricular telediastolic pressure lower than 20 mm of Hg; d) left ventricular function with ejection fraction not lesser than 0,40; e) absence of any associated severe and irreversible damages. The comparative data obtained recently from coronary patients medically and surgically treated would show at 4 years a survival rate higher than in those who underwent aorto-coronary by-pass.", "contents": "[Indications and contraindications for surgery in coronary insufficiency]. The indications to aortocoronary by-pass technique have during the last two years developed thanks to surgical, emodinamic and clinical exsperiences. The mortality of patients in whom surgery is limited electively to the intervention of aorto-coronary by-pass is at present around 4%. The principal indications to surgery are: a) existence of more than one coronary artery disease; b) a good diameter of the by-passing artery (more than 1 mm) corresponding to the site of the by-pass graft; c) a left ventricular telediastolic pressure lower than 20 mm of Hg; d) left ventricular function with ejection fraction not lesser than 0,40; e) absence of any associated severe and irreversible damages. The comparative data obtained recently from coronary patients medically and surgically treated would show at 4 years a survival rate higher than in those who underwent aorto-coronary by-pass."}
{"id": "PMID:1087390", "title": "Electrical stimulation in the relief of pain: a pilot study.", "content": "A group of 26 patients who suffered from chronic pain resistant to other therapeutic measures, were given electrical stimulation in two forms. The first utilised penetrating acupuncture needles connected to a standard Chinese electrical impulse generator and the second method employed American transcutaneous stimulators with conducting skin electrodes. At long term follow-up 50 percent of patients treated exhibited sustained relief--20 percent when the device was used alone and a further 30 percent when it was combined with other forms of specific pain therapy. There was no difference in the response to needle acupuncture or transcutaneous stimulation. The best response was seen in patients with neuralgic disorders and painful musculo skeletal syndromes affecting the cervico thoracic region.", "contents": "Electrical stimulation in the relief of pain: a pilot study. A group of 26 patients who suffered from chronic pain resistant to other therapeutic measures, were given electrical stimulation in two forms. The first utilised penetrating acupuncture needles connected to a standard Chinese electrical impulse generator and the second method employed American transcutaneous stimulators with conducting skin electrodes. At long term follow-up 50 percent of patients treated exhibited sustained relief--20 percent when the device was used alone and a further 30 percent when it was combined with other forms of specific pain therapy. There was no difference in the response to needle acupuncture or transcutaneous stimulation. The best response was seen in patients with neuralgic disorders and painful musculo skeletal syndromes affecting the cervico thoracic region."}
{"id": "PMID:1087391", "title": "Hepatic cirrhosis, alcohol and haemorrhage.", "content": "Fifty consecutive patients with cirrhosis of the liver were investigated, including 29 with gastrointestinal haemorrhage. Over two-thirds of these patients gave a history of heavy alcohol intake. The necessity for fibre-endoscopy is stressed in that barium studies alone showed oesophageal varices in only 40 percent of patients and erosive lesions were often present. Gastric varices, demonstrated by gastrophotography in 25 percent of patients with portal hypertension, were not readily detectable by standard barium meal or endoscopy. Radio-isotope scanning of the liver and spleen was invaluable in supporting not only the diagnosis of cirrhosis, but also the presence of portal hypertension, since the spleen was palpable in only 50 percent of such patients.", "contents": "Hepatic cirrhosis, alcohol and haemorrhage. Fifty consecutive patients with cirrhosis of the liver were investigated, including 29 with gastrointestinal haemorrhage. Over two-thirds of these patients gave a history of heavy alcohol intake. The necessity for fibre-endoscopy is stressed in that barium studies alone showed oesophageal varices in only 40 percent of patients and erosive lesions were often present. Gastric varices, demonstrated by gastrophotography in 25 percent of patients with portal hypertension, were not readily detectable by standard barium meal or endoscopy. Radio-isotope scanning of the liver and spleen was invaluable in supporting not only the diagnosis of cirrhosis, but also the presence of portal hypertension, since the spleen was palpable in only 50 percent of such patients."}
{"id": "PMID:1087392", "title": "Histocompatibility antigens and immune responsiveness; their relevance to rheumatic diseases.", "content": "Associations between histocompatibility antigens and human diseases have been found. Some of the highest correlations are with the so-called \"rheumatoid variant\" disorders. Data gained from ummunogenetic studies in laboratory animals has been invoked in speculation on the significance of the high antigen associations in disease pathogenesis. While much of this data better supports theories on the aetiology of other conditions with less significant antigen associations, it is discussed here with reference to the rheumatic diseases.", "contents": "Histocompatibility antigens and immune responsiveness; their relevance to rheumatic diseases. Associations between histocompatibility antigens and human diseases have been found. Some of the highest correlations are with the so-called \"rheumatoid variant\" disorders. Data gained from ummunogenetic studies in laboratory animals has been invoked in speculation on the significance of the high antigen associations in disease pathogenesis. While much of this data better supports theories on the aetiology of other conditions with less significant antigen associations, it is discussed here with reference to the rheumatic diseases."}
{"id": "PMID:1087393", "title": "The etiology and drug therapy of kinesia: investigations by means of the coriolis effect under cyclizine.", "content": "The typical symptoms of kinesia were produced in 30 healthy test subjects by means of the Coriolis effect and the effect of cyclizine upon them was investigated in a single blind trial. The drug showed a clear effect on the autonomic symptoms (nausea) while there was no evidence of inhibition of the peripheral vestibular function.", "contents": "The etiology and drug therapy of kinesia: investigations by means of the coriolis effect under cyclizine. The typical symptoms of kinesia were produced in 30 healthy test subjects by means of the Coriolis effect and the effect of cyclizine upon them was investigated in a single blind trial. The drug showed a clear effect on the autonomic symptoms (nausea) while there was no evidence of inhibition of the peripheral vestibular function."}
{"id": "PMID:1087394", "title": "Galvanic vesitbular test.", "content": "Galvanic stimulation of the vestibular system provokes a nystagmus as well as a tendency to fall. The direction of these phenomena depends on the localization of the anode. In this study the value of the minimal current strength (threshold), which is able to provoke nystagmus with a constant frequency during at least 30 sec was determined. Also the effects of the lesions in the vestibular system on these threshold values were measured. The method of bipolar-biaruricular stimulation showed to be the most sensitive one. A striking difference was found in the thresholds in rabbits with a vestibular organ with a lesion on that side. In that case the threshold for a nystagmus directed to the side with the intact ear was at its lowest. The conclusion is drawn that the galvanic vestibular test is suitable for the detection of the localization of a vestibular lesion in patients, especially when this lesion is located in the peripheral vestibular organ.", "contents": "Galvanic vesitbular test. Galvanic stimulation of the vestibular system provokes a nystagmus as well as a tendency to fall. The direction of these phenomena depends on the localization of the anode. In this study the value of the minimal current strength (threshold), which is able to provoke nystagmus with a constant frequency during at least 30 sec was determined. Also the effects of the lesions in the vestibular system on these threshold values were measured. The method of bipolar-biaruricular stimulation showed to be the most sensitive one. A striking difference was found in the thresholds in rabbits with a vestibular organ with a lesion on that side. In that case the threshold for a nystagmus directed to the side with the intact ear was at its lowest. The conclusion is drawn that the galvanic vestibular test is suitable for the detection of the localization of a vestibular lesion in patients, especially when this lesion is located in the peripheral vestibular organ."}
{"id": "PMID:1087395", "title": "[Cochleovestibular disorders after diving accidents].", "content": "Cochleo-vestibular lesions may result from decompression accidents and barotrauma. The etiology of both clinical syndromes as well as their therapy is discussed on the basis of seven personal observations.", "contents": "[Cochleovestibular disorders after diving accidents]. Cochleo-vestibular lesions may result from decompression accidents and barotrauma. The etiology of both clinical syndromes as well as their therapy is discussed on the basis of seven personal observations."}
{"id": "PMID:1087396", "title": "[Pendular and rotatory vestibular habituation].", "content": "The present investigations demonstrate in animals (rabbits) the existence of a habituation to the vestibular pendular stimulation comparable to the rotatory habituation by constant angular acceleration. There is no mutual transfer, however, between the two habituations. This confirms that the two modes of stimulation are not equivalent. All the tests have been made by ENG recording in total darkness.", "contents": "[Pendular and rotatory vestibular habituation]. The present investigations demonstrate in animals (rabbits) the existence of a habituation to the vestibular pendular stimulation comparable to the rotatory habituation by constant angular acceleration. There is no mutual transfer, however, between the two habituations. This confirms that the two modes of stimulation are not equivalent. All the tests have been made by ENG recording in total darkness."}
{"id": "PMID:1087397", "title": "[Optokinetic nystagmus studied through otoneurologic methods].", "content": "The horizontal optokinetic nystagmus (NOC) has been studied during 5,000 otoneurological investigations. In 16.25% of all our cases, the NOC was found to be either weakened in both directions, or only in one direction. There has been no constant relationship between intensity of the spontaneous nystagmus and NOC asymmetry in case of peripheral vestibular lesion. NOC asymmetry seems to be a reliable sign of the evolution of a lesion. Cases without peripheral hypofunction have been divided into three groups: ocular disturbances, brain stem lesions and hemispherical lesions. In these cases the value of the localization of the asymmetry in NOC and nystagmus induced by vestibular tests has been studied. In hemisperical lesions, when asymmetry affected exclusively the NOC, the direction of the weakened NOC was always towards  the healthy side. When asymmetry affected not only NOC but even nystagmus induced by vestibular stimulations, this weakened side of the nystagmus was less significant.", "contents": "[Optokinetic nystagmus studied through otoneurologic methods]. The horizontal optokinetic nystagmus (NOC) has been studied during 5,000 otoneurological investigations. In 16.25% of all our cases, the NOC was found to be either weakened in both directions, or only in one direction. There has been no constant relationship between intensity of the spontaneous nystagmus and NOC asymmetry in case of peripheral vestibular lesion. NOC asymmetry seems to be a reliable sign of the evolution of a lesion. Cases without peripheral hypofunction have been divided into three groups: ocular disturbances, brain stem lesions and hemispherical lesions. In these cases the value of the localization of the asymmetry in NOC and nystagmus induced by vestibular tests has been studied. In hemisperical lesions, when asymmetry affected exclusively the NOC, the direction of the weakened NOC was always towards  the healthy side. When asymmetry affected not only NOC but even nystagmus induced by vestibular stimulations, this weakened side of the nystagmus was less significant."}
{"id": "PMID:1087401", "title": "Upper gastrointestinal hemorrhage clinical, radiological and endoscopic correlation of 100 consecutive cases.", "content": "One hundred consecutive cases of upper gastrointestinal hemorrhage were studied clinically, radiologically and endoscopically. Erosive gastritis, duodenal and gastric ulcer, and bleeding esophageal varices accounted for 85% of the cases. The presenting sign of hematamesis or melena was of no value in localizing the bleeding site relative to the pyloric sphincter. Erosive lesions of the esophagus and stomach were suspected clinically in less than 50% of the cases and were the lesions least amenable to radiologic diagnosis and where early endoscopy was most useful. Our observations demonstrate again the frequent association between ethanol or aspirin ingestion and erosive gastritis.", "contents": "Upper gastrointestinal hemorrhage clinical, radiological and endoscopic correlation of 100 consecutive cases. One hundred consecutive cases of upper gastrointestinal hemorrhage were studied clinically, radiologically and endoscopically. Erosive gastritis, duodenal and gastric ulcer, and bleeding esophageal varices accounted for 85% of the cases. The presenting sign of hematamesis or melena was of no value in localizing the bleeding site relative to the pyloric sphincter. Erosive lesions of the esophagus and stomach were suspected clinically in less than 50% of the cases and were the lesions least amenable to radiologic diagnosis and where early endoscopy was most useful. Our observations demonstrate again the frequent association between ethanol or aspirin ingestion and erosive gastritis."}
{"id": "PMID:1087403", "title": "Nystagmic modulation of neuronal activity in rabbit cerebellar flocculus.", "content": "1. The responses of neuronal elements in the flocculus of the awake, restrained rabbit were recorded during horizontal vestibular nystagmus in the dark. 2. Purkinje cells showed both vestibular (Types I and II) and eye movement modulation of simple spike activity. Type I Purkinje cells most commonly were inhibited in association with the ipsilaterally directed fast phase of nystagmus and excited during contralaterally directed fast phases. Type II Purkinje cells had a similar modulation but in the opposite direction. Variations on this pattern included an increase in firing during fast phases in both directions. 3. Presumed mossy fibers and granule cells also exhibited both vestibular and nystagmic modulation in various combinations. The nystagmic modulation often began during the fast phase and continued into the early part of the slow phase. Mossy fibers showing both vestibular and nystagmic modulation probably arise from the vestibular nuclei and/or the perihypoglossal nuclei. 4. Floccular control of brain stem nuclei utilizes not only vestibular but also eye movement signals and probably all sensory and internal signals involved in the regulation of gaze.", "contents": "Nystagmic modulation of neuronal activity in rabbit cerebellar flocculus. 1. The responses of neuronal elements in the flocculus of the awake, restrained rabbit were recorded during horizontal vestibular nystagmus in the dark. 2. Purkinje cells showed both vestibular (Types I and II) and eye movement modulation of simple spike activity. Type I Purkinje cells most commonly were inhibited in association with the ipsilaterally directed fast phase of nystagmus and excited during contralaterally directed fast phases. Type II Purkinje cells had a similar modulation but in the opposite direction. Variations on this pattern included an increase in firing during fast phases in both directions. 3. Presumed mossy fibers and granule cells also exhibited both vestibular and nystagmic modulation in various combinations. The nystagmic modulation often began during the fast phase and continued into the early part of the slow phase. Mossy fibers showing both vestibular and nystagmic modulation probably arise from the vestibular nuclei and/or the perihypoglossal nuclei. 4. Floccular control of brain stem nuclei utilizes not only vestibular but also eye movement signals and probably all sensory and internal signals involved in the regulation of gaze."}
{"id": "PMID:1087404", "title": "Block of potassium channels of the nodal membrane by 4-aminopyridine and its partial removal on depolarization.", "content": "1. Voltage clamp experiments were done on single myelinated nerve fibres of the frog, Rana esculenta. 2. 53 muM 4-aminopyridine (4-AP) reduced IK to about one-fifth if tested with infrequent (1/min) and short (10 ms) depolarizing pulses; the onset time constant under these circumstances was ca. 160 s (14-15 degrees C). After prolonged treatment the effect was virtually irreversible. 3. At equilibrium with 4-AP, increasing the frequency of short pulses removed part of the block, the block removal accelerating with increasing pulse duration and frequency. 4. In 53 muM 4-AP unblocking of K channels during long (0.8 s) depolarizing pulses proceeded with a time constant, taur, of ca. 0.2 s. Restoration of block at the resting potential proceeded with a much larger time constant, tau'r, of ca. 1 min. 5. The stationary fraction, rinfinity, of K channels conducting in 53 muM 4-AP was 0.66, 0.41, and 0.24 at V = 120, 50, and 0 mV, respectively. 6. In a series of experiments with [4-AP] varying between 13.3 and 848 muM, taur decreased from 0.25 to 0.10 s (V = 130 mV, ca. 17 degrees C) while rinfinity followed the empirical relation 1/rinfinity = 1 + ct + cv exp(-0.77 EF/RT) with E = V - 70 mV. ct and cv are dimensionless quantities that increase with [4-AP] and reflect the voltage-independent and voltage-dependent component, respectively, of block. 7. Block of K channels and partial removal are also observed with inward IK at raised [K+]O. Removal proceeds on depolarization even if IK is additionally but temporarily suppressed by tetraethylammonium. Hence neither direction nor amplitude of IK but only the pulse potential seems to determine the extent of block for a given [4-AP].", "contents": "Block of potassium channels of the nodal membrane by 4-aminopyridine and its partial removal on depolarization. 1. Voltage clamp experiments were done on single myelinated nerve fibres of the frog, Rana esculenta. 2. 53 muM 4-aminopyridine (4-AP) reduced IK to about one-fifth if tested with infrequent (1/min) and short (10 ms) depolarizing pulses; the onset time constant under these circumstances was ca. 160 s (14-15 degrees C). After prolonged treatment the effect was virtually irreversible. 3. At equilibrium with 4-AP, increasing the frequency of short pulses removed part of the block, the block removal accelerating with increasing pulse duration and frequency. 4. In 53 muM 4-AP unblocking of K channels during long (0.8 s) depolarizing pulses proceeded with a time constant, taur, of ca. 0.2 s. Restoration of block at the resting potential proceeded with a much larger time constant, tau'r, of ca. 1 min. 5. The stationary fraction, rinfinity, of K channels conducting in 53 muM 4-AP was 0.66, 0.41, and 0.24 at V = 120, 50, and 0 mV, respectively. 6. In a series of experiments with [4-AP] varying between 13.3 and 848 muM, taur decreased from 0.25 to 0.10 s (V = 130 mV, ca. 17 degrees C) while rinfinity followed the empirical relation 1/rinfinity = 1 + ct + cv exp(-0.77 EF/RT) with E = V - 70 mV. ct and cv are dimensionless quantities that increase with [4-AP] and reflect the voltage-independent and voltage-dependent component, respectively, of block. 7. Block of K channels and partial removal are also observed with inward IK at raised [K+]O. Removal proceeds on depolarization even if IK is additionally but temporarily suppressed by tetraethylammonium. Hence neither direction nor amplitude of IK but only the pulse potential seems to determine the extent of block for a given [4-AP]."}
{"id": "PMID:1087406", "title": "In vitro colony growth of acute myelogenous leukemia.", "content": "Colony formation in vitro by marrow cells from patients with untreated acute myelogenous leukemia (AML) and from patients in AML relapse is infrequent using the standard Robinson assay. A newly developed culture system has been described in which marrow from AML patients in these disease stages form leukemic cell colonies. In this in vitro system, phytohaemagglutinin is the essential stimulator for colony formation. The leukemic origin of the colonies has been proven by ultrastructural morphology and cytogenetics. It appears that colony formation by leukemic cells in this system is predominantly independent from the leukocyte factor which is the main stimulator in the Robinson assay for growing colonies of marrow cells from haematologically normal individuals.", "contents": "In vitro colony growth of acute myelogenous leukemia. Colony formation in vitro by marrow cells from patients with untreated acute myelogenous leukemia (AML) and from patients in AML relapse is infrequent using the standard Robinson assay. A newly developed culture system has been described in which marrow from AML patients in these disease stages form leukemic cell colonies. In this in vitro system, phytohaemagglutinin is the essential stimulator for colony formation. The leukemic origin of the colonies has been proven by ultrastructural morphology and cytogenetics. It appears that colony formation by leukemic cells in this system is predominantly independent from the leukocyte factor which is the main stimulator in the Robinson assay for growing colonies of marrow cells from haematologically normal individuals."}
{"id": "PMID:1087407", "title": "Oncogenesis in human acute leukemia.", "content": "By selecting, for discussion, some aspects of oncogenesis in human acute leukemia, we hope to focus attention on areas of current interest which promise to provide important advances in our knowledge and understanding of human neoplasia, and, in time, may lead us nearer to the goal of disease control and eventual eradication.", "contents": "Oncogenesis in human acute leukemia. By selecting, for discussion, some aspects of oncogenesis in human acute leukemia, we hope to focus attention on areas of current interest which promise to provide important advances in our knowledge and understanding of human neoplasia, and, in time, may lead us nearer to the goal of disease control and eventual eradication."}
{"id": "PMID:1087408", "title": "Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNAs.", "content": "Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100-200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of phi80 plac DNA and phi80 plac DNA containing the Ll deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear lambdaplac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNA's revealed the absence of localized single-stranded regions.", "contents": "Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNAs. Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100-200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of phi80 plac DNA and phi80 plac DNA containing the Ll deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear lambdaplac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNA's revealed the absence of localized single-stranded regions."}
{"id": "PMID:1087412", "title": "[Hemorrhages of the digestive tract. Diagnosis and treatment using selective arteriography and perfusion of intra-arterial vasopressin].", "content": "Diagnosis of GI bleeding can safely be done by selective angiography of abdominal vessels. Patients are studied during acute bleeding phase with the Seldinger-Odman technique. In our experience we have a success rate of 89.4% in cases of arterial bleeding. Once diagnosis has been made, the selective catheter can be used to perform an infusion of a vasoconstrictive drug or selective embolization to treat the bleeding. This technique gives good results in case of arterial bleeding and is only a paliative method in case of esophageal varices.", "contents": "[Hemorrhages of the digestive tract. Diagnosis and treatment using selective arteriography and perfusion of intra-arterial vasopressin]. Diagnosis of GI bleeding can safely be done by selective angiography of abdominal vessels. Patients are studied during acute bleeding phase with the Seldinger-Odman technique. In our experience we have a success rate of 89.4% in cases of arterial bleeding. Once diagnosis has been made, the selective catheter can be used to perform an infusion of a vasoconstrictive drug or selective embolization to treat the bleeding. This technique gives good results in case of arterial bleeding and is only a paliative method in case of esophageal varices."}
{"id": "PMID:1087416", "title": "Regulation of the induction of colonies in vitro by normal human lymphocytes.", "content": "Lymphocytes isolated from normal human peripheral blood can be induced to form colonies in vitro by incubation with the appropriate inducer. Phytohemagglutinin can induce colonies with T (thymus-derived) lymphocytes. Optimun colony formation with about two colonies per 10(2) peripheral blood lymphocytes was obtained by adding, in addition to phytohemagglutinin, autologous plasma, autoologous red blood cells, and fresh L-glutamine or L-cystine. In the absence of these fresh amino acids, no colonies were formed at seeding levels below 10(5) cells per 35 mm petri dish. The addition of either of these amino acids gave a 10-fold decrease in the minimum number of cells that had to be seeded for colony formation. Lipopolysaccharide did not induce the formation of colonies, but enhanced the formation of T cell colonies by phytohemagglutinin. The mixing of lymphocytes from persons with and deficient in glucose-6-phosphate-dehydrogenase (EC 1-1-1-49) has shown that phytohemagglutinin-induced colonies can be derived from single cells and are, therefore, clones. No colonies were formed by lethally irradiated cells. Incubation with pokeweed mitogen also induced the formation of colonies. With autologous plasma and autologous red blood cells, pokeweek mitogen induced about one colony per 5 X 10(3) cells seeded and no colonies at seeding levels below 10(5) cells per petri dish. The minimum number of cells needed for colony formation by pokeweed mitogen was not decreased by fresh L-glutamine or L-cystine. The results indicate that normal human lymphocytes can be cloned in vitro and that induction of these lymphocyte colonies can be regulated by lectins and other specific factors.", "contents": "Regulation of the induction of colonies in vitro by normal human lymphocytes. Lymphocytes isolated from normal human peripheral blood can be induced to form colonies in vitro by incubation with the appropriate inducer. Phytohemagglutinin can induce colonies with T (thymus-derived) lymphocytes. Optimun colony formation with about two colonies per 10(2) peripheral blood lymphocytes was obtained by adding, in addition to phytohemagglutinin, autologous plasma, autoologous red blood cells, and fresh L-glutamine or L-cystine. In the absence of these fresh amino acids, no colonies were formed at seeding levels below 10(5) cells per 35 mm petri dish. The addition of either of these amino acids gave a 10-fold decrease in the minimum number of cells that had to be seeded for colony formation. Lipopolysaccharide did not induce the formation of colonies, but enhanced the formation of T cell colonies by phytohemagglutinin. The mixing of lymphocytes from persons with and deficient in glucose-6-phosphate-dehydrogenase (EC 1-1-1-49) has shown that phytohemagglutinin-induced colonies can be derived from single cells and are, therefore, clones. No colonies were formed by lethally irradiated cells. Incubation with pokeweed mitogen also induced the formation of colonies. With autologous plasma and autologous red blood cells, pokeweek mitogen induced about one colony per 5 X 10(3) cells seeded and no colonies at seeding levels below 10(5) cells per petri dish. The minimum number of cells needed for colony formation by pokeweed mitogen was not decreased by fresh L-glutamine or L-cystine. The results indicate that normal human lymphocytes can be cloned in vitro and that induction of these lymphocyte colonies can be regulated by lectins and other specific factors."}
{"id": "PMID:1087413", "title": "[Immunoblastic lymphadenopathy and toxacariasis. Report of a case].", "content": "This is a report of a case a larval granulomatosis associated with immunoblastic lymphadenopathy, corroborated by laboratory and histologic studies compatible with that. The etiopathogenesis, clinical picture and diagnosis of both diseases are commented, and the possible relationship between them is stressed.", "contents": "[Immunoblastic lymphadenopathy and toxacariasis. Report of a case]. This is a report of a case a larval granulomatosis associated with immunoblastic lymphadenopathy, corroborated by laboratory and histologic studies compatible with that. The etiopathogenesis, clinical picture and diagnosis of both diseases are commented, and the possible relationship between them is stressed."}
{"id": "PMID:1087418", "title": "The concept of prevalence applied to psychiatric disorders and symptoms.", "content": "Statements about prevalence, of the type 'x% of the general population are mentally ill', imply a concept of prevalence that is difficult to define operationally. It requires the specification of a cut-off point, a level of severity from which the presence of an illness can be inferred, and this is largely arbitrary. A more generally useful type of statement compares the frequency distribution of severity for declared cases with that for the rest of the population. This avoids the need for a defined cut-off point. We are still left with the problem of defining a dimension of severity and a declared case, but these should follow easily from a clear statement of the purpose of any specific inquiry. The argument is illustrated from the results of a study of self-referrals to general practitioners.", "contents": "The concept of prevalence applied to psychiatric disorders and symptoms. Statements about prevalence, of the type 'x% of the general population are mentally ill', imply a concept of prevalence that is difficult to define operationally. It requires the specification of a cut-off point, a level of severity from which the presence of an illness can be inferred, and this is largely arbitrary. A more generally useful type of statement compares the frequency distribution of severity for declared cases with that for the rest of the population. This avoids the need for a defined cut-off point. We are still left with the problem of defining a dimension of severity and a declared case, but these should follow easily from a clear statement of the purpose of any specific inquiry. The argument is illustrated from the results of a study of self-referrals to general practitioners."}
{"id": "PMID:1087420", "title": "A study of the factors influencing mortality rates from gastrointestinal haemorrhage.", "content": "A prospective study is reported of 300 consecutive patients admitted to the General Hospital, Birmingham, because of acute gastrointestinal haemorrhage. The characteristics of the group have been outlined and the causes of death examined. It is shown that in patients diagnosed as having peptic ulcer or erosions, the major causes of death were thrombotic vascular disease and surgical complications. Current policies in the management of gastrointestinal bleeding are examined in this light, and it is concluded that gastric resection should be avoided wherever possible, and that procedures should be considered which might reduce intravascular clotting. The advisability of immediate surgery is also questioned and a case made for consideration of a policy of more prolonged resuscitation. A detailed analysis of mortality rates is included from reported European series, with particular reference to the increasing proportion of older patients. Taking this into consideration, it is argued that mortality rates are continuing to improve, and that early diagnosis must be an important contributory factor. It is also pointed out that not only are nearly half the patients in present studies over the age of sixty, but also the risk of developing such a haemorrhage is much greater in the older sections of the community. Gastrointestinal bleeding is more of a geriatric than an adolescent problem.", "contents": "A study of the factors influencing mortality rates from gastrointestinal haemorrhage. A prospective study is reported of 300 consecutive patients admitted to the General Hospital, Birmingham, because of acute gastrointestinal haemorrhage. The characteristics of the group have been outlined and the causes of death examined. It is shown that in patients diagnosed as having peptic ulcer or erosions, the major causes of death were thrombotic vascular disease and surgical complications. Current policies in the management of gastrointestinal bleeding are examined in this light, and it is concluded that gastric resection should be avoided wherever possible, and that procedures should be considered which might reduce intravascular clotting. The advisability of immediate surgery is also questioned and a case made for consideration of a policy of more prolonged resuscitation. A detailed analysis of mortality rates is included from reported European series, with particular reference to the increasing proportion of older patients. Taking this into consideration, it is argued that mortality rates are continuing to improve, and that early diagnosis must be an important contributory factor. It is also pointed out that not only are nearly half the patients in present studies over the age of sixty, but also the risk of developing such a haemorrhage is much greater in the older sections of the community. Gastrointestinal bleeding is more of a geriatric than an adolescent problem."}
{"id": "PMID:1087441", "title": "Hodgkin's disease: a war between T-lymphocytes and transformed macrophages?", "content": "The interaction between Reed-Sternberg (RS) cells and autogeneic T T cells was investigated. It was found that T cells cytolyse RS cells in the following sequential manner: Stage 1, T cells affix the tips of their microvilli onto target cells, Stage 2, T cells subject cell membranes to tearing, and shearing forces which produce gaps and holes; Stage 3, target cells lyse and T cells \"crawl\" away. The relationship between the events occurring at the cellular level and those observed clinically are discussed. Hodgkin's disease holds a fascination for clinicians and \"bench\" scientists alike, perhaps because of the enigma and challenge it represents. It is not surprising, therefore, that many questions go unanswered. For example, we are still addressing ourselves to the following questions: What is the origin of the Reed-Sternberg (RS) cell? If a macrophage, then why do T cells attach to it and what is the nature of the T cell-RS interaction? Why is cellular immunity so often impaired even in patients in the early stages of the disease? Why do patients with Hodgkin's disease sometimes develop autoimmune hemolytic anemia when they are, in fact, immunologically hyporesponsive? In this paper, I shall present evidence which answers a few of these questions and provides hypothetical answers to the others.", "contents": "Hodgkin's disease: a war between T-lymphocytes and transformed macrophages? The interaction between Reed-Sternberg (RS) cells and autogeneic T T cells was investigated. It was found that T cells cytolyse RS cells in the following sequential manner: Stage 1, T cells affix the tips of their microvilli onto target cells, Stage 2, T cells subject cell membranes to tearing, and shearing forces which produce gaps and holes; Stage 3, target cells lyse and T cells \"crawl\" away. The relationship between the events occurring at the cellular level and those observed clinically are discussed. Hodgkin's disease holds a fascination for clinicians and \"bench\" scientists alike, perhaps because of the enigma and challenge it represents. It is not surprising, therefore, that many questions go unanswered. For example, we are still addressing ourselves to the following questions: What is the origin of the Reed-Sternberg (RS) cell? If a macrophage, then why do T cells attach to it and what is the nature of the T cell-RS interaction? Why is cellular immunity so often impaired even in patients in the early stages of the disease? Why do patients with Hodgkin's disease sometimes develop autoimmune hemolytic anemia when they are, in fact, immunologically hyporesponsive? In this paper, I shall present evidence which answers a few of these questions and provides hypothetical answers to the others."}
{"id": "PMID:1087442", "title": "The significance of the macrophage content of human tumours.", "content": "The effective localization of an immunogenic rat sarcoma (HSBPA) is related to its macrophage content. In order to infiltrate this tumous with macrophages, T-cell function is important. In a tumour of low immunogenicity, MC3, with a low macrophage content, thymectomy has little effect on the ease with which such a tumour metastasizes. The monocytes in the peripheral blood of a rat myeloid leukaemia are of host origin and are significantly raised during the growth of the leukaemia. Similar monocytoid cells are found in human acute myelogenous leukaemias and appear to be important in the prognostic features of this type of leukaemia. The origin of these monocytoid cells has not been elucidated. In 56 solid human tumours the macrophage content of metastatic tumours is low (less than 10%) whereas the range in primary or locally recurrent tumours is 0-30%. It is too early to say whether the low macrophage infiltrated primary will readily metastasize compared to the high macrophage containing tumour.", "contents": "The significance of the macrophage content of human tumours. The effective localization of an immunogenic rat sarcoma (HSBPA) is related to its macrophage content. In order to infiltrate this tumous with macrophages, T-cell function is important. In a tumour of low immunogenicity, MC3, with a low macrophage content, thymectomy has little effect on the ease with which such a tumour metastasizes. The monocytes in the peripheral blood of a rat myeloid leukaemia are of host origin and are significantly raised during the growth of the leukaemia. Similar monocytoid cells are found in human acute myelogenous leukaemias and appear to be important in the prognostic features of this type of leukaemia. The origin of these monocytoid cells has not been elucidated. In 56 solid human tumours the macrophage content of metastatic tumours is low (less than 10%) whereas the range in primary or locally recurrent tumours is 0-30%. It is too early to say whether the low macrophage infiltrated primary will readily metastasize compared to the high macrophage containing tumour."}
{"id": "PMID:1087444", "title": "Increase in \"Null\" cells in acute lymphocytic leukemia in remission on long-term immunotherapy.", "content": "In an attempt to elucidate a possible mode of action of immunotherapy in ALL, we studied the mononuclear subpopulation in a group of patients with all in remission on long-term immunotherapy, a similar group on chemotherapy, and a group of normal individuals. Comparison of the three groups demonstrated a significant increase in the number and percentage of \"null\" cells in the immunotherapy group relative to the two others. Although the nature of these cells is unknown, circulating stem cells, \"K\" cells, or modified T, B, or monocytic cells are raised.", "contents": "Increase in \"Null\" cells in acute lymphocytic leukemia in remission on long-term immunotherapy. In an attempt to elucidate a possible mode of action of immunotherapy in ALL, we studied the mononuclear subpopulation in a group of patients with all in remission on long-term immunotherapy, a similar group on chemotherapy, and a group of normal individuals. Comparison of the three groups demonstrated a significant increase in the number and percentage of \"null\" cells in the immunotherapy group relative to the two others. Although the nature of these cells is unknown, circulating stem cells, \"K\" cells, or modified T, B, or monocytic cells are raised."}
{"id": "PMID:1087445", "title": "Revised seminology of the different mononuclear cells under scanning electron microscopy.", "content": "Scanning electron microscopy shows that the thymus cell surface is not smooth but slightly undulated; this type of surface characteristic is found on the cells of several types of T-lymphocyte tumors such as T-cell lymphosarcoma, mycosis fungoides, and T-immunoblastic lymphosarcoma. The cell surface of Frabricius' bursa is covered with numerous short villi. They are found on the cells of several kinds of B-lymphocyte tumors, including B-cell chronic lymphoid leukemia, B-prolymphocytic lymphosarcoma, and B-immunoblastic lymphosarcoma. Mycosis fungoides cells frequently have a characteristic shape. Normal and myeloma plasma cells are similar in shape but their surface is covered with numberous characteristic tiny balls. Immunoblasts are twice the size of so-called small lymphocytes. In the blood and in the lymphocyte population transformed by mitogens, cell surfaces vary from completely smooth (with no undulations as in thymic cells) to villous, with short to very long villi. These variations are visible even when a constant technique and the critical-point drying method are used. Their significance, however, is not known.", "contents": "Revised seminology of the different mononuclear cells under scanning electron microscopy. Scanning electron microscopy shows that the thymus cell surface is not smooth but slightly undulated; this type of surface characteristic is found on the cells of several types of T-lymphocyte tumors such as T-cell lymphosarcoma, mycosis fungoides, and T-immunoblastic lymphosarcoma. The cell surface of Frabricius' bursa is covered with numerous short villi. They are found on the cells of several kinds of B-lymphocyte tumors, including B-cell chronic lymphoid leukemia, B-prolymphocytic lymphosarcoma, and B-immunoblastic lymphosarcoma. Mycosis fungoides cells frequently have a characteristic shape. Normal and myeloma plasma cells are similar in shape but their surface is covered with numberous characteristic tiny balls. Immunoblasts are twice the size of so-called small lymphocytes. In the blood and in the lymphocyte population transformed by mitogens, cell surfaces vary from completely smooth (with no undulations as in thymic cells) to villous, with short to very long villi. These variations are visible even when a constant technique and the critical-point drying method are used. Their significance, however, is not known."}
{"id": "PMID:1087448", "title": "Integrated EMG feedback in the management of spasmodic torticollis and focal dystonia: a prospective study of 80 patients.", "content": "In summary, then, without consideration of specific circuits or transmitter agents, one can conceive of a hypothetical model that involves both learning and the functional nature of the defect in torticollis and focal dystonia to describe the results obtained. The model must be further elaborated upon and tested, preferably in a quantitative manner. Naturally, the specific finding of a defective transmitter agent (e.g., GABA) such as described in parkinsonian syndrome (dopamine) or the interruption of a specific pathway that causes and improves a dyskinesia is desirable. In this chapter we have described the use of integrated EMG feedback for the treatment of focal dystonia or spasmodic torticollis. Although we have achieved significant results, it remains clear that further research in the treatment of these disorders is required. However, since this treatment does not require medication or surgery and the possibility for significant improvement is greater than 40%, it should be attempted in patients with focal dystonia or torticollis prior to other forms of therapy. SFT should be considered as a standard mode in the medical armamentarium used for the treatment of these disorders, either primarily or in conjunction with other forms of medical, surgical, and physical therapy.", "contents": "Integrated EMG feedback in the management of spasmodic torticollis and focal dystonia: a prospective study of 80 patients. In summary, then, without consideration of specific circuits or transmitter agents, one can conceive of a hypothetical model that involves both learning and the functional nature of the defect in torticollis and focal dystonia to describe the results obtained. The model must be further elaborated upon and tested, preferably in a quantitative manner. Naturally, the specific finding of a defective transmitter agent (e.g., GABA) such as described in parkinsonian syndrome (dopamine) or the interruption of a specific pathway that causes and improves a dyskinesia is desirable. In this chapter we have described the use of integrated EMG feedback for the treatment of focal dystonia or spasmodic torticollis. Although we have achieved significant results, it remains clear that further research in the treatment of these disorders is required. However, since this treatment does not require medication or surgery and the possibility for significant improvement is greater than 40%, it should be attempted in patients with focal dystonia or torticollis prior to other forms of therapy. SFT should be considered as a standard mode in the medical armamentarium used for the treatment of these disorders, either primarily or in conjunction with other forms of medical, surgical, and physical therapy."}
{"id": "PMID:1087450", "title": "Simultaneous determination of arsenic, copper, manganese, selenium, and zinc in biological materials by neutron activation analysis.", "content": "A method for the simultaneous determination of arsenic, copper, manganese, selenium, and zinc in biological material was developed by the incorporation of separation procedures for copper and zinc into an existing procedure. Investigation of the performance characteristics of the method was carried out with reference to copper and zinc. For certain materials characterized by a high Cu/Zn ratio, or a high zinc content, or both, such as liver, copper interferes in the determination of zinc thus requiring a small correction by an iterative procedure. Blank values for copper depend on the rinsing of the irradiation container, and a single rinsing with redistilled water was found superior to other rinsing procedures. Nuclear interference was negligible. The accuracy of the method was checked by analysis of Standard Reference Materials and the precision verified by analysis of Intercomparison Samples. Results are presented for 5 male foetuses of 3-5 months' gestational age. The distribution of arsenic, manganese and selenium is similar to that previously reported for adults. With the exception of liver, concentrations of copper in foetal organs were lower than values in the literature indicate.", "contents": "Simultaneous determination of arsenic, copper, manganese, selenium, and zinc in biological materials by neutron activation analysis. A method for the simultaneous determination of arsenic, copper, manganese, selenium, and zinc in biological material was developed by the incorporation of separation procedures for copper and zinc into an existing procedure. Investigation of the performance characteristics of the method was carried out with reference to copper and zinc. For certain materials characterized by a high Cu/Zn ratio, or a high zinc content, or both, such as liver, copper interferes in the determination of zinc thus requiring a small correction by an iterative procedure. Blank values for copper depend on the rinsing of the irradiation container, and a single rinsing with redistilled water was found superior to other rinsing procedures. Nuclear interference was negligible. The accuracy of the method was checked by analysis of Standard Reference Materials and the precision verified by analysis of Intercomparison Samples. Results are presented for 5 male foetuses of 3-5 months' gestational age. The distribution of arsenic, manganese and selenium is similar to that previously reported for adults. With the exception of liver, concentrations of copper in foetal organs were lower than values in the literature indicate."}
{"id": "PMID:1087452", "title": "Biologic and analytic components of variation of concentration values of selected serum proteins.", "content": "The magnitude and statistical significance of the various biologic and analytic sources of variation of the serum concentration values of seven proteins: albumin, alpha1-antitrypsin, transferrin, orosomucoid, IgG, IgM, and IgA, were evaluated in 12 healthy subjects. Blood specimens were obtained at 0800 h, 1100 h, and 1400 h on one particular day. The analytic error was partitioned into pre-instrumental and instrumental components; the biologic variation was separated into inter-subject variation of mean levels and intra-individual within-day variation. The mean levels of the subjects differed significantly for all seven proteins studied. For the group as a whole there were no consistent diurnal variations of significance. However, for all the proteins, except for IgM, significant individual within-day biologic fluctuations were noted. The within-day biologic variation was smaller than the total analytic variation for transferrin, IgG, orosomucoid, and IgM. When the total analytic variation was separated into pre-instrumental and instrumental components, it was found that the pre-instrumental sources of variation were of a relatively greater magnitude for albumin, IgG, IgA, alpha1-antitrypsin, and IgM.", "contents": "Biologic and analytic components of variation of concentration values of selected serum proteins. The magnitude and statistical significance of the various biologic and analytic sources of variation of the serum concentration values of seven proteins: albumin, alpha1-antitrypsin, transferrin, orosomucoid, IgG, IgM, and IgA, were evaluated in 12 healthy subjects. Blood specimens were obtained at 0800 h, 1100 h, and 1400 h on one particular day. The analytic error was partitioned into pre-instrumental and instrumental components; the biologic variation was separated into inter-subject variation of mean levels and intra-individual within-day variation. The mean levels of the subjects differed significantly for all seven proteins studied. For the group as a whole there were no consistent diurnal variations of significance. However, for all the proteins, except for IgM, significant individual within-day biologic fluctuations were noted. The within-day biologic variation was smaller than the total analytic variation for transferrin, IgG, orosomucoid, and IgM. When the total analytic variation was separated into pre-instrumental and instrumental components, it was found that the pre-instrumental sources of variation were of a relatively greater magnitude for albumin, IgG, IgA, alpha1-antitrypsin, and IgM."}
{"id": "PMID:1087453", "title": "Interaction between normal and leukaemic human cells in agar culture.", "content": "The influence of leukaemic cells from 12 patients with acute leukaemia on normal granulopoiesis in agar culture was investigated using leukeamic cell feeder layers. Leukaemic feeder cells from 7 of the 12 patients elicited no colony growth, while cells from the remaining 5 stimulated normal colony growth. In 3 of the 7 non-stimulatory patients release of inhibitory factors from the leukaemic cells seemed responsible for the effect on normal granulopoiesis, while inappropriate colony stimulating factor (CSF) production by the feeder cells could not be ruled out in the remaining 4 patients. When the leukaemic cells were cultured with, as well as without, conditioned medium, cells from 5 of the patients formed clusters. Growth in these cultures did not correlate to the effect found in the feeder layer experiments.", "contents": "Interaction between normal and leukaemic human cells in agar culture. The influence of leukaemic cells from 12 patients with acute leukaemia on normal granulopoiesis in agar culture was investigated using leukeamic cell feeder layers. Leukaemic feeder cells from 7 of the 12 patients elicited no colony growth, while cells from the remaining 5 stimulated normal colony growth. In 3 of the 7 non-stimulatory patients release of inhibitory factors from the leukaemic cells seemed responsible for the effect on normal granulopoiesis, while inappropriate colony stimulating factor (CSF) production by the feeder cells could not be ruled out in the remaining 4 patients. When the leukaemic cells were cultured with, as well as without, conditioned medium, cells from 5 of the patients formed clusters. Growth in these cultures did not correlate to the effect found in the feeder layer experiments."}
{"id": "PMID:1087454", "title": "The effect of syngeneic peripheral blood cells on the formation of colonies by normal human bone marrow cells in diffusion chambers in mice.", "content": "This paper describes the influence of cells capable of releasing colony stimulating activity (CSA) in vitro on the formation of granulocytic colonies by normal human bone marrow in diffusion chambers in mice. A carbonyl iron method was used to remove phagocytic cells from normal human bone marrow. This treatment prevented spontaneous colony and cluster formation when the cells were cultured in agar in vitro at initial concentrations of 2-5 x 105 cells per ml. However, non-phagocytic bone marrow cells formed granulocytic colonies when inoculated into diffusion chambers at 105 cells per chamber and cultured in 450 R-irradiated or non-irradiated mice. The formation of granulocytic colonies by carbonyl iron treated marrow in diffusion chamber cultures was not consistently enhanced by the admixture of 1.4 x 105 1500 R-irradiated syngeneic light density blood cells (less than 1.077 g/ml) to the chamber inoculum. In contrast, these cells induced cluster or colony formation when added in the same proportion to the marrow cells in agar cultures in vitro. Addition of 1.4 x 105 1500 R-irradiated high density blood cells(greater than 1.077 g/ml) to the inoculum resulted in a slight, non-significant decrease in the number of colonies in diffusion chambers. The stimulating effect of host irradiation on neutrophilic colony formation was independent of the presence of CSA releasing cells in the chamber inoculum.", "contents": "The effect of syngeneic peripheral blood cells on the formation of colonies by normal human bone marrow cells in diffusion chambers in mice. This paper describes the influence of cells capable of releasing colony stimulating activity (CSA) in vitro on the formation of granulocytic colonies by normal human bone marrow in diffusion chambers in mice. A carbonyl iron method was used to remove phagocytic cells from normal human bone marrow. This treatment prevented spontaneous colony and cluster formation when the cells were cultured in agar in vitro at initial concentrations of 2-5 x 105 cells per ml. However, non-phagocytic bone marrow cells formed granulocytic colonies when inoculated into diffusion chambers at 105 cells per chamber and cultured in 450 R-irradiated or non-irradiated mice. The formation of granulocytic colonies by carbonyl iron treated marrow in diffusion chamber cultures was not consistently enhanced by the admixture of 1.4 x 105 1500 R-irradiated syngeneic light density blood cells (less than 1.077 g/ml) to the chamber inoculum. In contrast, these cells induced cluster or colony formation when added in the same proportion to the marrow cells in agar cultures in vitro. Addition of 1.4 x 105 1500 R-irradiated high density blood cells(greater than 1.077 g/ml) to the inoculum resulted in a slight, non-significant decrease in the number of colonies in diffusion chambers. The stimulating effect of host irradiation on neutrophilic colony formation was independent of the presence of CSA releasing cells in the chamber inoculum."}
{"id": "PMID:1087455", "title": "Changes in the surface of virus-infected cells recognized by cytotoxic T cells. II. A requirement for glycoprotein synthesis in virus-infected target cells.", "content": "Infection of cells with either ectromelia or lymphocytic choriomeningitis (LCM) virus in the presence of 2-deoxy-D-glucose (2-DOG) inhibited by up to 70% the extent to which the infected cells become susceptible to virus-specific cell-mediated lysis. The concentration of 2-DOG used had little effect on the extent of total protein synthesis (incorporation of [35S] methionine) but inhibited (up to 25%) glycoprotein synthesis, as measured by incorporation of [3H] fucose. This suggested that glycoprotein synthesis was a necessary event for infected cells to become susceptible to T-cell mediated lysis. The profiles (polyacrylamide gel electrophoresis) of newly synthesized, cellular glycoproteins from unifected and ectromelia-infected cells in the presence and absence of 2-DOG were compared and found to be very complex, with only minor changes. However, when convalescent serum from infected mice was used to isolate newly synthesized components from the cell surface shortly after infection, it showed four main species ranging in size from 25,000 to 70,000 daltons. 2-DOG inhibited production of these by 70%, thus corresponding to the biological data. The nature of the new glycoproteins seen in infected cells and whether they are in fact the structures recognized by effector T cells remain to be determined.", "contents": "Changes in the surface of virus-infected cells recognized by cytotoxic T cells. II. A requirement for glycoprotein synthesis in virus-infected target cells. Infection of cells with either ectromelia or lymphocytic choriomeningitis (LCM) virus in the presence of 2-deoxy-D-glucose (2-DOG) inhibited by up to 70% the extent to which the infected cells become susceptible to virus-specific cell-mediated lysis. The concentration of 2-DOG used had little effect on the extent of total protein synthesis (incorporation of [35S] methionine) but inhibited (up to 25%) glycoprotein synthesis, as measured by incorporation of [3H] fucose. This suggested that glycoprotein synthesis was a necessary event for infected cells to become susceptible to T-cell mediated lysis. The profiles (polyacrylamide gel electrophoresis) of newly synthesized, cellular glycoproteins from unifected and ectromelia-infected cells in the presence and absence of 2-DOG were compared and found to be very complex, with only minor changes. However, when convalescent serum from infected mice was used to isolate newly synthesized components from the cell surface shortly after infection, it showed four main species ranging in size from 25,000 to 70,000 daltons. 2-DOG inhibited production of these by 70%, thus corresponding to the biological data. The nature of the new glycoproteins seen in infected cells and whether they are in fact the structures recognized by effector T cells remain to be determined."}
{"id": "PMID:1087456", "title": "[Demonstration and analysis of immune complexes in rheumatic diseases].", "content": "By means of an immunofluorescence technique, immune complexes have been demonstrated in circulating blood leukocytes, in serum and in joint effusions of patients with various rheumatic diseases. Such complexes are present within cell inclusions in about 30% of patients with rheumatoid arthritis (RA). RA patients has immune complexes in serum in 75% of cases and in joint effusions in over 80% of cases. Soluble immune complexes were demonstrated in the serum of about 30% of age-matched patients with degenerative joint disease, while such complexes are present in the serum of only 1.5% of a younger, normal control group. The immune complexes chiefly contain IgG, frequently IgM and only rarely IgA. CO complement component can frequently be demonstrated within such complexes. In RA patients a statistically significant correlation (p less than 0.001) could be established between the presence of soluble immune complexes and erythrocyte sedimentation rate, but no correlation was observed between the presence of such complexes and the Lansbury joint index. The possible pathogenetic role of immune complex clearance by circulating leukocytes in rheumatoid arthritis is discussed.", "contents": "[Demonstration and analysis of immune complexes in rheumatic diseases]. By means of an immunofluorescence technique, immune complexes have been demonstrated in circulating blood leukocytes, in serum and in joint effusions of patients with various rheumatic diseases. Such complexes are present within cell inclusions in about 30% of patients with rheumatoid arthritis (RA). RA patients has immune complexes in serum in 75% of cases and in joint effusions in over 80% of cases. Soluble immune complexes were demonstrated in the serum of about 30% of age-matched patients with degenerative joint disease, while such complexes are present in the serum of only 1.5% of a younger, normal control group. The immune complexes chiefly contain IgG, frequently IgM and only rarely IgA. CO complement component can frequently be demonstrated within such complexes. In RA patients a statistically significant correlation (p less than 0.001) could be established between the presence of soluble immune complexes and erythrocyte sedimentation rate, but no correlation was observed between the presence of such complexes and the Lansbury joint index. The possible pathogenetic role of immune complex clearance by circulating leukocytes in rheumatoid arthritis is discussed."}
{"id": "PMID:1087457", "title": "[Therapy of inflammatory-rheumatic diseases with levamisol, an immunity modulating substance].", "content": "Since cellular and humoral immune mechanisms were shown to be involved in the pathogenesis of rheumatic diseases, efforts have been made to influence these systems therapeutically. Aside from suppression of the immune response, studies have been conducted to evaluate to what extent immunostimulation might be of value in the treatment of rheumatic diseases. Levamisole, chemically a simple synthetic agent recently shown to have immunorestorative capacities in anergic cancer patients, has been administered in several rheumatic diseases. The drug was administered either continuously or intermittently in a daily dose of 150 mg over several months. In about half of the rheumatoid arthritis patients a significant improvement was noted. Beneficial effects and sometimes even dramatic improvement lere observed in some patients with systemic lupus erythematosus, ankylosing spondylitis and Reiter's syndrome. Levamisole did not alter the course of psoriatic arthritis. Significant adverse reactions were leukopenia and allergic skin reactions. Other side effects were mild and did not require interruption of drug intake. The use of levamisole in rheumatic diseases is still experimental, but with accumulating experience it may provide a true improvement in control of rheumatic diseases.", "contents": "[Therapy of inflammatory-rheumatic diseases with levamisol, an immunity modulating substance]. Since cellular and humoral immune mechanisms were shown to be involved in the pathogenesis of rheumatic diseases, efforts have been made to influence these systems therapeutically. Aside from suppression of the immune response, studies have been conducted to evaluate to what extent immunostimulation might be of value in the treatment of rheumatic diseases. Levamisole, chemically a simple synthetic agent recently shown to have immunorestorative capacities in anergic cancer patients, has been administered in several rheumatic diseases. The drug was administered either continuously or intermittently in a daily dose of 150 mg over several months. In about half of the rheumatoid arthritis patients a significant improvement was noted. Beneficial effects and sometimes even dramatic improvement lere observed in some patients with systemic lupus erythematosus, ankylosing spondylitis and Reiter's syndrome. Levamisole did not alter the course of psoriatic arthritis. Significant adverse reactions were leukopenia and allergic skin reactions. Other side effects were mild and did not require interruption of drug intake. The use of levamisole in rheumatic diseases is still experimental, but with accumulating experience it may provide a true improvement in control of rheumatic diseases."}
{"id": "PMID:1087458", "title": "Essential role of macrophages in chronic inflammatory processes.", "content": "It is clear that the macrophage plays a central role in the mediation of chronic inflammatory processes. The discussion below only alludes to certain of the mediators secreted by macrophages which contribute to chronic inflammation, and the reader is referred to other sources for further discussion of these and others. The combined activities of these products may account in large part for the effects of macrophages on its pericellular environment and neighboring cells during chronic inflammation.", "contents": "Essential role of macrophages in chronic inflammatory processes. It is clear that the macrophage plays a central role in the mediation of chronic inflammatory processes. The discussion below only alludes to certain of the mediators secreted by macrophages which contribute to chronic inflammation, and the reader is referred to other sources for further discussion of these and others. The combined activities of these products may account in large part for the effects of macrophages on its pericellular environment and neighboring cells during chronic inflammation."}
{"id": "PMID:1087459", "title": "In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules.", "content": "Two neutral proteinases, polymorphonuclear leukocyte (PMN) elastase and cathepsin G, were purified from azurophil granules of human PMN. Both enzymes were found to stimulate both human and mouse lymphocytes in vitro. Experiments with mouse cells showed that the PMN proteinases are B-cell stimulants. Stimulation appears to depend on direct proteolytic action on the lymphocyte surface and not to require the mediation of serum factors of the help of T-cells.", "contents": "In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules. Two neutral proteinases, polymorphonuclear leukocyte (PMN) elastase and cathepsin G, were purified from azurophil granules of human PMN. Both enzymes were found to stimulate both human and mouse lymphocytes in vitro. Experiments with mouse cells showed that the PMN proteinases are B-cell stimulants. Stimulation appears to depend on direct proteolytic action on the lymphocyte surface and not to require the mediation of serum factors of the help of T-cells."}
{"id": "PMID:1087460", "title": "Anti--DNA antibodies in patients with leukemia and lymphoma.", "content": "217 sera from 33 patients with acute myeloid leukemia (AML), 42 with chronic myeloid leukemia (CML), 12 with acute lymphatic leukemia (ALL), 22 with lymphoma, and 20 with other malignant diseases were examined by a radioimmunological technique for the presence of antibodies against DNA. The levels of single-stranded DNA binding activity was significantly higher in all 3 types of leukemia and lymphoma compared to those of healthy controls. In contrast, none of these sera exhibited a positive reaction with double-stranded DNA. In some cases of leukemia, the level of serum anti-DNA antibodies increased after the decrease of the leukocytes count.", "contents": "Anti--DNA antibodies in patients with leukemia and lymphoma. 217 sera from 33 patients with acute myeloid leukemia (AML), 42 with chronic myeloid leukemia (CML), 12 with acute lymphatic leukemia (ALL), 22 with lymphoma, and 20 with other malignant diseases were examined by a radioimmunological technique for the presence of antibodies against DNA. The levels of single-stranded DNA binding activity was significantly higher in all 3 types of leukemia and lymphoma compared to those of healthy controls. In contrast, none of these sera exhibited a positive reaction with double-stranded DNA. In some cases of leukemia, the level of serum anti-DNA antibodies increased after the decrease of the leukocytes count."}
{"id": "PMID:1087461", "title": "[Spontaneous hemobilia in the differential diagnosis of upper gastrointestinal hemorrhages].", "content": "Hemobilia, biliary tree hemorrhage, is characterized by melana and/or haematemesis, biliary colic and clinical jaundice. The rare cause of gastrointestinal bleeding probably accounts for quite a large percentage of gastrointestinal hemorrhages of undetermined source. In the evaluation of gastrointestinal bleeding the hepato-biliary system is seldom examined unless trauma has occurred. However, 10-15% of cases with hemobilia are non-traumatic. Three cases are presented illustrating three different causes of non-traumatic hemobilia observed during the last three years. The diagnostic problems and the therapeutic possibilities are described.", "contents": "[Spontaneous hemobilia in the differential diagnosis of upper gastrointestinal hemorrhages]. Hemobilia, biliary tree hemorrhage, is characterized by melana and/or haematemesis, biliary colic and clinical jaundice. The rare cause of gastrointestinal bleeding probably accounts for quite a large percentage of gastrointestinal hemorrhages of undetermined source. In the evaluation of gastrointestinal bleeding the hepato-biliary system is seldom examined unless trauma has occurred. However, 10-15% of cases with hemobilia are non-traumatic. Three cases are presented illustrating three different causes of non-traumatic hemobilia observed during the last three years. The diagnostic problems and the therapeutic possibilities are described."}
{"id": "PMID:1087462", "title": "[Psychsomatic aspects of rheumatic, especially soft tissue rheumatic diseases].", "content": "Rheumatic diseases may be connected to varying degrees with psychic factors. On the one hand, rheumatic diseases may produce psychic reactions, while on the other hand rheumatic symptoms--especially in extra-articular rheumatism--may be induced by psychosomatic hysteric pararheumatic diseases and depressive mechanisms. Special reference is made to psychosomatically induced extra-articular rheumatic diseases in which not only the complex genesis but also the clinical symptomatology are discussed. During therapy more attention should be directed to psychic factors than in the past, as only an intensive psycho and/or psychopharmacotherapy can influence the disease.", "contents": "[Psychsomatic aspects of rheumatic, especially soft tissue rheumatic diseases]. Rheumatic diseases may be connected to varying degrees with psychic factors. On the one hand, rheumatic diseases may produce psychic reactions, while on the other hand rheumatic symptoms--especially in extra-articular rheumatism--may be induced by psychosomatic hysteric pararheumatic diseases and depressive mechanisms. Special reference is made to psychosomatically induced extra-articular rheumatic diseases in which not only the complex genesis but also the clinical symptomatology are discussed. During therapy more attention should be directed to psychic factors than in the past, as only an intensive psycho and/or psychopharmacotherapy can influence the disease."}
{"id": "PMID:1087468", "title": "The prevalence of anaemia and iron deficiency in the Indian community in Natal.", "content": "A prevalence study of iron deficiency and anaemia was done among the Indian population of metropolitan Durban. In all, 2500 subjects were studied. This survey confirms previous impressions of a high incidence of anaemia and iron deficiency. Iron deficiency was observed in 33% of females and 20% of males. Anaemia was observed in 38,1% of females and 44,3% of males. A high incidence of anaemia was also found in children under 10 years of age-28,6% for females and 41,6% for males.", "contents": "The prevalence of anaemia and iron deficiency in the Indian community in Natal. A prevalence study of iron deficiency and anaemia was done among the Indian population of metropolitan Durban. In all, 2500 subjects were studied. This survey confirms previous impressions of a high incidence of anaemia and iron deficiency. Iron deficiency was observed in 33% of females and 20% of males. Anaemia was observed in 38,1% of females and 44,3% of males. A high incidence of anaemia was also found in children under 10 years of age-28,6% for females and 41,6% for males."}
{"id": "PMID:1087469", "title": "Computed tomography in the diagnosis of colloid cyst.", "content": "The diagnosis of colloid cyst of the third ventricle is notoriously difficult and contributes significantly to the high mortality. Three cases are presented in which computed tomography has contributed to the diagnosis by showing (1) the dilatation of the lateral ventricles without dilatation of the midline ventricular system, and (2) the lesion as an area of increased density within the third ventricle. Computed tomography may reduce the overall mortality in this condition by reducing the number of patients who succumb undiagnosed during an acute phase of raised intracranial pressure.", "contents": "Computed tomography in the diagnosis of colloid cyst. The diagnosis of colloid cyst of the third ventricle is notoriously difficult and contributes significantly to the high mortality. Three cases are presented in which computed tomography has contributed to the diagnosis by showing (1) the dilatation of the lateral ventricles without dilatation of the midline ventricular system, and (2) the lesion as an area of increased density within the third ventricle. Computed tomography may reduce the overall mortality in this condition by reducing the number of patients who succumb undiagnosed during an acute phase of raised intracranial pressure."}
{"id": "PMID:1087470", "title": "Amyloidoma of the gasserian ganglion.", "content": "A case report, the third in the literature, is presented of a patient whose progressive numbness in the second and third divisions of the trigeminal nerve led to the discovery of an isolated amyloidoma of the gasserian ganglion. The clinical impression of tumor was confirmed by surgical and pathologic findings.", "contents": "Amyloidoma of the gasserian ganglion. A case report, the third in the literature, is presented of a patient whose progressive numbness in the second and third divisions of the trigeminal nerve led to the discovery of an isolated amyloidoma of the gasserian ganglion. The clinical impression of tumor was confirmed by surgical and pathologic findings."}
{"id": "PMID:1087471", "title": "Simultaneous subdural effusion and hydrocephalus in infancy.", "content": "Hydrocephalus and subdural hematoma or effusion of infancy rarely present simultaneously, where both are active contributors to acutely increased intracranial pressure. In three cases, clinical findings characteristic of both were present. Decompression of one can facilitate expansion of the other. Rapid progression of unsuspected hydrocephalus could be responsible for some of the poor results reported after treatment of subdural effusion alone. This possibility should be considered whenever progress is unsatisfactory during treatment of subdural effusion.", "contents": "Simultaneous subdural effusion and hydrocephalus in infancy. Hydrocephalus and subdural hematoma or effusion of infancy rarely present simultaneously, where both are active contributors to acutely increased intracranial pressure. In three cases, clinical findings characteristic of both were present. Decompression of one can facilitate expansion of the other. Rapid progression of unsuspected hydrocephalus could be responsible for some of the poor results reported after treatment of subdural effusion alone. This possibility should be considered whenever progress is unsatisfactory during treatment of subdural effusion."}
{"id": "PMID:1087472", "title": "Endarterectomy of the left anterior descending and mainstem coronary arteries: a technique for reconstruction of inoperable arteries.", "content": "The proximal left anterior descending coronary artery (PLAD) is an area of predilection for such severe and diffuse calcific arteriosclerosis that reconstruction of these vessels often is impossible. The branches of this segment include the septal perforators, median artery, the left anterior descending coronary artery, and its first and second diagonal branches. Successful endarterectomy, therefore, would revascularize large areas of the left ventricle and interventricular septum. We have performed 45 such operations during the past 13 months. Following endarterectomy there are several methods of reconstructing the endarterectomized vessel, the preferable technique being the addition of a saphenous vein bypass to the endarterectomized segment. Patients selected for this operation were mostly in the fair (58%) and poor risk (42%) categories; there were no good risk patients. Diffuse arterial disease was the rule. The average ejection fraction was 0.48. The operation was successful with respect to graft patency, bypass flow rates, and symptomatic relief. The operative mortality rate in the entire group was 15%, including the 19 poor risk patients in six of whom elective preoperative use of an antra-aortic balloon pump was required. Most of the surviving patients (92%) were either symptom free or greatly improved. Only two patients were clinically unchanged. There was one late sudden death. This operation is indicated when there is extensive involvement of the life main, the proximal left anterior descending coronary artery and its major branches. It is the only possible way to revascularize otherwise inoperable arteries.", "contents": "Endarterectomy of the left anterior descending and mainstem coronary arteries: a technique for reconstruction of inoperable arteries. The proximal left anterior descending coronary artery (PLAD) is an area of predilection for such severe and diffuse calcific arteriosclerosis that reconstruction of these vessels often is impossible. The branches of this segment include the septal perforators, median artery, the left anterior descending coronary artery, and its first and second diagonal branches. Successful endarterectomy, therefore, would revascularize large areas of the left ventricle and interventricular septum. We have performed 45 such operations during the past 13 months. Following endarterectomy there are several methods of reconstructing the endarterectomized vessel, the preferable technique being the addition of a saphenous vein bypass to the endarterectomized segment. Patients selected for this operation were mostly in the fair (58%) and poor risk (42%) categories; there were no good risk patients. Diffuse arterial disease was the rule. The average ejection fraction was 0.48. The operation was successful with respect to graft patency, bypass flow rates, and symptomatic relief. The operative mortality rate in the entire group was 15%, including the 19 poor risk patients in six of whom elective preoperative use of an antra-aortic balloon pump was required. Most of the surviving patients (92%) were either symptom free or greatly improved. Only two patients were clinically unchanged. There was one late sudden death. This operation is indicated when there is extensive involvement of the life main, the proximal left anterior descending coronary artery and its major branches. It is the only possible way to revascularize otherwise inoperable arteries."}
{"id": "PMID:1087478", "title": "Family studies of patients with reduced ristocetin aggregation and abnormalities of factor VIII and/or platelet function.", "content": "Factor VIII procoagulant activity (VIIIc), antigen (vWa), mobility of the antigen on two dimensional immunoelectrophoresis and platelet function were studied in 9 families with reduced ristocetin induced platelet aggregation rate (RIPA) and/or deficiency of plasma factor(s) required for ristocetin aggregation of washed normal platelets (vWf). the families could be subdivided into 4 groups. Group I showed dominant inheritance and reduced levels of VIIIc and vWa characteristic of typical von Willebrand's disease. All patients had reduced vWf and in 7 of 10 RIPA was reduced. Group II showed normal levels of VIIIc but reduced vWa. All showed reduced vWf but RIPA was reduced in one patient only. There was a good correlation between vWf and vWa and VIIIc in both groups. The bleeding time correlated with vWf in group I but not group II. Group III showed normal or nearly normal VIIIc and vWa but there was an increased mobility of vWa compared to normals and to groups I and II. RIPA was markedly reduced as was the vWf in one patient. Group IV is represented by one child with a strong family history of bleeding, who had reduced RIPA and defective platelet release reaction. The vWf in this child was normal and the ratio between VIIIc and vWa was similar to that seen in carriers of haemophilia. This spectrum of abnormalities of ristocetin aggregation justifies the use of the term 'von Willebrand's syndrome'.", "contents": "Family studies of patients with reduced ristocetin aggregation and abnormalities of factor VIII and/or platelet function. Factor VIII procoagulant activity (VIIIc), antigen (vWa), mobility of the antigen on two dimensional immunoelectrophoresis and platelet function were studied in 9 families with reduced ristocetin induced platelet aggregation rate (RIPA) and/or deficiency of plasma factor(s) required for ristocetin aggregation of washed normal platelets (vWf). the families could be subdivided into 4 groups. Group I showed dominant inheritance and reduced levels of VIIIc and vWa characteristic of typical von Willebrand's disease. All patients had reduced vWf and in 7 of 10 RIPA was reduced. Group II showed normal levels of VIIIc but reduced vWa. All showed reduced vWf but RIPA was reduced in one patient only. There was a good correlation between vWf and vWa and VIIIc in both groups. The bleeding time correlated with vWf in group I but not group II. Group III showed normal or nearly normal VIIIc and vWa but there was an increased mobility of vWa compared to normals and to groups I and II. RIPA was markedly reduced as was the vWf in one patient. Group IV is represented by one child with a strong family history of bleeding, who had reduced RIPA and defective platelet release reaction. The vWf in this child was normal and the ratio between VIIIc and vWa was similar to that seen in carriers of haemophilia. This spectrum of abnormalities of ristocetin aggregation justifies the use of the term 'von Willebrand's syndrome'."}
{"id": "PMID:1087479", "title": "Release, aggregation and lysis of human platelets by antilymphocyte globulin and antiplatelet serum.", "content": "Human platelets labeled with 51Cr were used to determine the contribution made by platelet lysis to the platelet release reaction and platelet aggregation induced by rabbit antihuman platelet serum (APS) and equine antihuman thymocyte globulin (ATG). Platelets were tested in both plasma (PRP) and non-plasma containing media. Antibodies directed against platelets, either as APS or ATG, induced significant amounts of platelet release and aggregation, as well as some degree of lysis, in the absence of complement. The presence of complement increased platelet lysis and aggregation, but not the release reaction. Non-immune horse gamma globulin produced different responses depending upon whether platelets were investigated in PRP or non-plasma containing media. Aggregation was seen in the latter but not the former. These differences can be explained by the presence of plasma components which prevent non-specific immune complexes from causing platelet aggregation. Since platelets in vivo are always in a plasma medium, one must be wary of utilizing data from platelet studies in synthetic plasma-free media as the basis of explaining clinical events. These observations demonstrate at least two, and possibly three, different mechanisms whereby ATG could activate platelets causing thrombotic complications and thrombocytopenia, i.e., via 1) specific and, 2) non-specific non-lytic pathways and 3) a lytic pathway.", "contents": "Release, aggregation and lysis of human platelets by antilymphocyte globulin and antiplatelet serum. Human platelets labeled with 51Cr were used to determine the contribution made by platelet lysis to the platelet release reaction and platelet aggregation induced by rabbit antihuman platelet serum (APS) and equine antihuman thymocyte globulin (ATG). Platelets were tested in both plasma (PRP) and non-plasma containing media. Antibodies directed against platelets, either as APS or ATG, induced significant amounts of platelet release and aggregation, as well as some degree of lysis, in the absence of complement. The presence of complement increased platelet lysis and aggregation, but not the release reaction. Non-immune horse gamma globulin produced different responses depending upon whether platelets were investigated in PRP or non-plasma containing media. Aggregation was seen in the latter but not the former. These differences can be explained by the presence of plasma components which prevent non-specific immune complexes from causing platelet aggregation. Since platelets in vivo are always in a plasma medium, one must be wary of utilizing data from platelet studies in synthetic plasma-free media as the basis of explaining clinical events. These observations demonstrate at least two, and possibly three, different mechanisms whereby ATG could activate platelets causing thrombotic complications and thrombocytopenia, i.e., via 1) specific and, 2) non-specific non-lytic pathways and 3) a lytic pathway."}
{"id": "PMID:1087480", "title": "[Bronchoplasty in tumor surgery (author's transl)].", "content": "A series of 80 bronchial resections and other broncho-plasties is presented; 60 operations were performed on the right side, while only 20 on the left side. The most frequent variation was the \"sleeve lobectomy\" (upper lobe with main bronchus). The attention is called to other possibilities so as: the reinplantation of the middle lobe after removal of the lower lobe and the intermede bronchus, the anastomosis between the left main bronchus and the upper lobe bronchus when the dividsion of the main bronchus must be removed together with the lower lobe, the repair of the continuity on the right side by means of a double anastomosis etc. Lethal postoperative complications occurred in 7 cases; 29 patients died in consequence of recidives or metastases and 44 are alive (22 more than 3 years, 8 more than 5 years). These procedures may significantly enlarge the resectability of the cancer in old patients and in those with limited respiratory reserve.", "contents": "[Bronchoplasty in tumor surgery (author's transl)]. A series of 80 bronchial resections and other broncho-plasties is presented; 60 operations were performed on the right side, while only 20 on the left side. The most frequent variation was the \"sleeve lobectomy\" (upper lobe with main bronchus). The attention is called to other possibilities so as: the reinplantation of the middle lobe after removal of the lower lobe and the intermede bronchus, the anastomosis between the left main bronchus and the upper lobe bronchus when the dividsion of the main bronchus must be removed together with the lower lobe, the repair of the continuity on the right side by means of a double anastomosis etc. Lethal postoperative complications occurred in 7 cases; 29 patients died in consequence of recidives or metastases and 44 are alive (22 more than 3 years, 8 more than 5 years). These procedures may significantly enlarge the resectability of the cancer in old patients and in those with limited respiratory reserve."}
{"id": "PMID:1087481", "title": "[Experimental investigation of cardiac lymph vascular system (author's transl)].", "content": "Ligatures of the supracardiac lymph vessels and the cardiac lymph nodes have been performed in fifty mongrel dogs. Comparative electrocardiographical, light microscopical, scanning and transmission electron microscopical investigations were done one day to three months after surgery. The demonstrated findings confirmed former results concerning the topography and the microstructure of the cardiac lymph vascular drainage system. In addition, the comparative functional and morphological examinations made evident the pathogenetic importance of cardiac lymphostasis. Lymphostatic oedema has been discussed as a pathogenetic co-factor for human heart diseases. Therapeutic attempts on experimental cardiac lymphostasis with i.v. - application of Coumarin-Troxerutin were without effect.", "contents": "[Experimental investigation of cardiac lymph vascular system (author's transl)]. Ligatures of the supracardiac lymph vessels and the cardiac lymph nodes have been performed in fifty mongrel dogs. Comparative electrocardiographical, light microscopical, scanning and transmission electron microscopical investigations were done one day to three months after surgery. The demonstrated findings confirmed former results concerning the topography and the microstructure of the cardiac lymph vascular drainage system. In addition, the comparative functional and morphological examinations made evident the pathogenetic importance of cardiac lymphostasis. Lymphostatic oedema has been discussed as a pathogenetic co-factor for human heart diseases. Therapeutic attempts on experimental cardiac lymphostasis with i.v. - application of Coumarin-Troxerutin were without effect."}
{"id": "PMID:1087482", "title": "[Long-term cardiac arrest by cardioplegic coronary perfusion (author's transl)].", "content": "The Mg++ aspartate-procaine-cardioplegia has been proven in animal experiments as well as from 1970 til 1975 in more than 1000 open-heart-procedures by a myocardial temperature of 32 degrees C and aortic crossclamping time up to 40 minutes superior to all other known procedures of cardiac preservations. To guarantee a safe myocardial protection of the arrested heart for a remarkable longer period of total ischemia, we further developed the cardioplegic technique in the animal lab, and use it now clinically. The arrest is induced by cardioplegia (Mg++ aspartate-procaine), than the arrested heart is cooled down to 15-20 degrees C by cardioplegic coronary perfusion maintaining the oxidative metabolism. The perfusion is stopped. The begin of ischemia is still under normal ATP-levels and continuous cardioplegia. 1. Crossclamping of the aorta; 2. cardioplegic induced cardiac arrest by Mg++ aspartate-procaine (Kirsch); 3. Surface cooling of the heart; 4. Coronary perfusion by hypothermic cardioplegic solution (8-10 min, flow 80-120 ml/min, perfusion pressure maximal 30 mmHg). Perfusate: O2-saturated, erythrocyte free, 6% hydroxyethyl starch solution added 2 mM Mg++ aspartate, 4 mM procaine, 50 mM Na+, 5 mM K+, 0,5 mM Ca++, 25 mM HCO3-, 10 mM glucose, 200 mM mannitol, 250 mg/l 6-methylprednisolone. 84 patients (29 ACVB; 55 valve replacements); crossclamping time: 71 min (SD 22); total time of ischemia: 57 min (SD 18; max 96, min. 27 min); reperfusion time restoring normal excitation-contraction of the heart: 3 min (SD 2); weaning off bypass: 23 min (SD 14). Hemodynamic 12 h postop.: SO2 venous 76% (SD 6). No sympathicomimetics were used. Only 30% of myocardial ATP is splitted after 120 min of cardiac arrest. Electron microscopic findings show only small, reversible alterations of fine structure.", "contents": "[Long-term cardiac arrest by cardioplegic coronary perfusion (author's transl)]. The Mg++ aspartate-procaine-cardioplegia has been proven in animal experiments as well as from 1970 til 1975 in more than 1000 open-heart-procedures by a myocardial temperature of 32 degrees C and aortic crossclamping time up to 40 minutes superior to all other known procedures of cardiac preservations. To guarantee a safe myocardial protection of the arrested heart for a remarkable longer period of total ischemia, we further developed the cardioplegic technique in the animal lab, and use it now clinically. The arrest is induced by cardioplegia (Mg++ aspartate-procaine), than the arrested heart is cooled down to 15-20 degrees C by cardioplegic coronary perfusion maintaining the oxidative metabolism. The perfusion is stopped. The begin of ischemia is still under normal ATP-levels and continuous cardioplegia. 1. Crossclamping of the aorta; 2. cardioplegic induced cardiac arrest by Mg++ aspartate-procaine (Kirsch); 3. Surface cooling of the heart; 4. Coronary perfusion by hypothermic cardioplegic solution (8-10 min, flow 80-120 ml/min, perfusion pressure maximal 30 mmHg). Perfusate: O2-saturated, erythrocyte free, 6% hydroxyethyl starch solution added 2 mM Mg++ aspartate, 4 mM procaine, 50 mM Na+, 5 mM K+, 0,5 mM Ca++, 25 mM HCO3-, 10 mM glucose, 200 mM mannitol, 250 mg/l 6-methylprednisolone. 84 patients (29 ACVB; 55 valve replacements); crossclamping time: 71 min (SD 22); total time of ischemia: 57 min (SD 18; max 96, min. 27 min); reperfusion time restoring normal excitation-contraction of the heart: 3 min (SD 2); weaning off bypass: 23 min (SD 14). Hemodynamic 12 h postop.: SO2 venous 76% (SD 6). No sympathicomimetics were used. Only 30% of myocardial ATP is splitted after 120 min of cardiac arrest. Electron microscopic findings show only small, reversible alterations of fine structure."}
{"id": "PMID:1087483", "title": "[Controlling of artificial blood pumps after total heart replacement - an example of disregulation (author's transl)].", "content": "This is a report on the special regulation problem of the left blood pump after replacement of the natural heart by incorporated extracorporally driven blood pumps in an animal experiment. The consequence of the peripheral self-regulation on the transporting capacity of the bloodpumps considering the driving pressure and the systemic pressure losses has been investigated. Two possible controlling principles and the respective fields of application are discussed on the example of a lung oedema.", "contents": "[Controlling of artificial blood pumps after total heart replacement - an example of disregulation (author's transl)]. This is a report on the special regulation problem of the left blood pump after replacement of the natural heart by incorporated extracorporally driven blood pumps in an animal experiment. The consequence of the peripheral self-regulation on the transporting capacity of the bloodpumps considering the driving pressure and the systemic pressure losses has been investigated. Two possible controlling principles and the respective fields of application are discussed on the example of a lung oedema."}
{"id": "PMID:1087484", "title": "[Possibilities of pacing the heart by transmediastinal retrocardially inserted leads (author's transl)].", "content": "Based upon experiences with 14 patients the clinical feasibility of transmediastinal retrocardial stimulation of the heart, of the left atrium as well as in one case of the left ventricle, is reported. Principally both can be stimulated by thus introduced pacemaker electodes. Thus on the one hand thresholds and consequently pulse effectivity appear to be a function of the electrode distance from the myocardium, whilst on the other hand the transmediastinal retrocardial thresholds are initially rather high in analogy to the majority taken from the atrial endocardial surface. Nevertheless, the transmediastinal retrocardial technique is possibly applicable for permanent cardiac pacing. However, additional tests about this are still necessary.", "contents": "[Possibilities of pacing the heart by transmediastinal retrocardially inserted leads (author's transl)]. Based upon experiences with 14 patients the clinical feasibility of transmediastinal retrocardial stimulation of the heart, of the left atrium as well as in one case of the left ventricle, is reported. Principally both can be stimulated by thus introduced pacemaker electodes. Thus on the one hand thresholds and consequently pulse effectivity appear to be a function of the electrode distance from the myocardium, whilst on the other hand the transmediastinal retrocardial thresholds are initially rather high in analogy to the majority taken from the atrial endocardial surface. Nevertheless, the transmediastinal retrocardial technique is possibly applicable for permanent cardiac pacing. However, additional tests about this are still necessary."}
{"id": "PMID:1087485", "title": "[A simple method for tricuspid annuloplasty (author's transl)].", "content": "A new operative method for tricuspid annuloplasty is reported. In this method the antero-posterior portion of the tricuspid annulus is sutured to a semi-circular nonelastic strip of teflon by double needle sutures. Since the radius of this strip is shorter than the radius of the valve ring, the tricuspid annulus will be narrowed after the sutures are tied. Because of its simplicity, firmness and maintenance of the valvular ring elasticity, this method seems to be advantageous to all similar procedures. The results of the method in 5 patients with severe tricuspid incompetence have been excellent. This method can also be used for annuloplasty of the mitral valve.", "contents": "[A simple method for tricuspid annuloplasty (author's transl)]. A new operative method for tricuspid annuloplasty is reported. In this method the antero-posterior portion of the tricuspid annulus is sutured to a semi-circular nonelastic strip of teflon by double needle sutures. Since the radius of this strip is shorter than the radius of the valve ring, the tricuspid annulus will be narrowed after the sutures are tied. Because of its simplicity, firmness and maintenance of the valvular ring elasticity, this method seems to be advantageous to all similar procedures. The results of the method in 5 patients with severe tricuspid incompetence have been excellent. This method can also be used for annuloplasty of the mitral valve."}
{"id": "PMID:1087486", "title": "[Congenital aneurysms of the membranous ventricular septum (author's transl)].", "content": "In 3 patients with a congenital aneurysm of the membranous portion of the ventricular septum diagnosis could be made by angiography. One of these patients was clinically asymptomatic, while the two other patients were transferred for surgery because of right ventricular outflow tract obstruction or intracardiac left-to-right shunt. These both patients underwent successful open heart corrections. Aneurysms with a different intracardiac localization could be found. One of the cases represents an unusual association with corrected transposition of the great vessels.", "contents": "[Congenital aneurysms of the membranous ventricular septum (author's transl)]. In 3 patients with a congenital aneurysm of the membranous portion of the ventricular septum diagnosis could be made by angiography. One of these patients was clinically asymptomatic, while the two other patients were transferred for surgery because of right ventricular outflow tract obstruction or intracardiac left-to-right shunt. These both patients underwent successful open heart corrections. Aneurysms with a different intracardiac localization could be found. One of the cases represents an unusual association with corrected transposition of the great vessels."}
{"id": "PMID:1087487", "title": "[Two stage surgical treatment of large ventricular septal defects (author's transl)].", "content": "Since March 1971, 51 infants were subjected to pulmonary artery banding (PAB) for a large ventricular septal defect (VSD) with pulmonary hypertension. 41 infants (80%) were under six months of age. Additional defects were present in 41%. Twelve babies died (24%). The lowest mortality was achieved in isolated VSD (6,7%). 28 patients subsequently underwent VSD closure and pulmonary artery debanding. Catheterization data revealed normal or slightly elevated pressures and normal vascular resistance in the pulmonary circuit in 22 children. The operative mortality rate was 10,7%.", "contents": "[Two stage surgical treatment of large ventricular septal defects (author's transl)]. Since March 1971, 51 infants were subjected to pulmonary artery banding (PAB) for a large ventricular septal defect (VSD) with pulmonary hypertension. 41 infants (80%) were under six months of age. Additional defects were present in 41%. Twelve babies died (24%). The lowest mortality was achieved in isolated VSD (6,7%). 28 patients subsequently underwent VSD closure and pulmonary artery debanding. Catheterization data revealed normal or slightly elevated pressures and normal vascular resistance in the pulmonary circuit in 22 children. The operative mortality rate was 10,7%."}
{"id": "PMID:1087488", "title": "[Aneurysm of pericardial right ventricular outflow tract patches (author's transl)].", "content": "Among 1022 patients who underwent total repair of tetralogy of Fallot, 252 patients received a pericardial patch of the right ventricular outflow tract (RVOT); and of these 10 subsequently developed an aneurysm of the RVOT. Cardiac catheterization and angiography revealed moderate pulmonary unsufficiency in all patients, a residual pressure gradient in the RVOT in seven and a residual ventricular septal defect in two patients. Surgery was indicated in eight patients because of progressive distension of the aneurysm. Reconstruction of the RVOT was accomplished by resection of the aneurysm and insertion of a woven Dacron patch in five patients, primary suture of the pulmonary artery in two and implantation of a woven Dacron conduit containing a Bj\u00f6rk-Shiley valve in one patient. No early or late death occured. Although aneurysms of the RVOT with pericardial patches represent a rare complication after total correction of tetralogy of Fallot, we recommend primary reconstruction of the RVOT with a woven Dacron patch.", "contents": "[Aneurysm of pericardial right ventricular outflow tract patches (author's transl)]. Among 1022 patients who underwent total repair of tetralogy of Fallot, 252 patients received a pericardial patch of the right ventricular outflow tract (RVOT); and of these 10 subsequently developed an aneurysm of the RVOT. Cardiac catheterization and angiography revealed moderate pulmonary unsufficiency in all patients, a residual pressure gradient in the RVOT in seven and a residual ventricular septal defect in two patients. Surgery was indicated in eight patients because of progressive distension of the aneurysm. Reconstruction of the RVOT was accomplished by resection of the aneurysm and insertion of a woven Dacron patch in five patients, primary suture of the pulmonary artery in two and implantation of a woven Dacron conduit containing a Bj\u00f6rk-Shiley valve in one patient. No early or late death occured. Although aneurysms of the RVOT with pericardial patches represent a rare complication after total correction of tetralogy of Fallot, we recommend primary reconstruction of the RVOT with a woven Dacron patch."}
{"id": "PMID:1087489", "title": "[Survival of 51cr-tagged red blood cells in patients with artificial heart valves (author's transl)].", "content": "Survival of 51Cr-tagged erythrocytes is determined in 10 patients approximately 1 week after insertion of an artificial heart valve (\"early hemolysis\") and in 9 patients approximately 2 years after heart valve replacement (\"late hemolysis\"). Red blood cell survival is found to be significantly reduced in all patients following insertion of an artificial heart valve as compared to a group of control patients. Hemolysis is significantly more pronounced in the early postoperative phase than two years postoperatively. LDH and plasma hemoglobin are relatively good indicators of hemolysis but less reliable than the determination of t/2 Cr.", "contents": "[Survival of 51cr-tagged red blood cells in patients with artificial heart valves (author's transl)]. Survival of 51Cr-tagged erythrocytes is determined in 10 patients approximately 1 week after insertion of an artificial heart valve (\"early hemolysis\") and in 9 patients approximately 2 years after heart valve replacement (\"late hemolysis\"). Red blood cell survival is found to be significantly reduced in all patients following insertion of an artificial heart valve as compared to a group of control patients. Hemolysis is significantly more pronounced in the early postoperative phase than two years postoperatively. LDH and plasma hemoglobin are relatively good indicators of hemolysis but less reliable than the determination of t/2 Cr."}
{"id": "PMID:1087492", "title": "Sinus histiocytosis with massive lymphadenopathy.", "content": "Five cases of \"sinus histiocytosis with massive lymphadenopathy\" are reported. The clinical features and histopathological appearances are described. The majority were African children with massive cervical lymphadenopathy. Histologically, the lymphnode sinuses are widely dilated by mature histiocytes, with phagocytosis of lymphocytes and other hematopoietic cells by the proliferating histiocytes. The diagnostic difficulties are discussed. These are the first cases recognized in Zambia.", "contents": "Sinus histiocytosis with massive lymphadenopathy. Five cases of \"sinus histiocytosis with massive lymphadenopathy\" are reported. The clinical features and histopathological appearances are described. The majority were African children with massive cervical lymphadenopathy. Histologically, the lymphnode sinuses are widely dilated by mature histiocytes, with phagocytosis of lymphocytes and other hematopoietic cells by the proliferating histiocytes. The diagnostic difficulties are discussed. These are the first cases recognized in Zambia."}
{"id": "PMID:1087493", "title": "Electronic bladder stimulation in spinal cord paralysis.", "content": "Authors performed electronic stimulation of the paralysed bladder that was attributed to various innervation troubles. For this purpose, they implanted 10 patients with stimulator Model PMS-3, each with 8 electrodes. In three cases, outside factors (like endocarditis, pyelonephritis aposthematosa, and progress of paralysis in the limbs) forced them to remove the stimulator. The other cases can be declared as successful, for complete bladder emptying has been obtained. The authors want to emphasize that patients with an implanted stimulator can dispense with an indwelling catheter thereby avoiding vesicoureteral reflux. The absence of recurrent pyelonephritis results in prolonged health. This new technique seems to be appropriate, especially in peripheric paralysis, while central paralysis connected with fibrosis of the bladder neck often requires additional surgical intervention, e.g. transurethral resection.", "contents": "Electronic bladder stimulation in spinal cord paralysis. Authors performed electronic stimulation of the paralysed bladder that was attributed to various innervation troubles. For this purpose, they implanted 10 patients with stimulator Model PMS-3, each with 8 electrodes. In three cases, outside factors (like endocarditis, pyelonephritis aposthematosa, and progress of paralysis in the limbs) forced them to remove the stimulator. The other cases can be declared as successful, for complete bladder emptying has been obtained. The authors want to emphasize that patients with an implanted stimulator can dispense with an indwelling catheter thereby avoiding vesicoureteral reflux. The absence of recurrent pyelonephritis results in prolonged health. This new technique seems to be appropriate, especially in peripheric paralysis, while central paralysis connected with fibrosis of the bladder neck often requires additional surgical intervention, e.g. transurethral resection."}
{"id": "PMID:1087494", "title": "Evaluation of cellular immunologic responsiveness in the clinical management of patients with prostatic cancer. II. Effect of oestrogen, cryosurgery and transurethral resection on thymic-dependent lymphocytic blastogenesis.", "content": "The effect of oestrogen, cryosurgery and transurethral resection (TUR) of the prostate on the blastogenic response of thymic-dependent peripheral blood lymphocytes (PBL) to the non-specific mitogen, phytohaemagglutinin (PHA) was evaluated as one in vitro criteria of each of these treatment modalities on the cellular immunologic responsiveness of 24 patients with prostatic cancer. A depression 5 days following receipt of oestrogen and 2-7 days following cryosurgery or TUR of the responsiveness of PHA-stimulated PBL was observed. Oestrogen-induced aberrations of responsiveness may not only be of relevance in prostatic cancer patients, but also suggested association between uterine cancer and prolonged administration of diethylstilboesterol and the development of vaginal tumours in offspring found in association with maternal ingestion during pregnancy. Particularly striking was that contrary to the reduced responsiveness of PBL cultured in autologous and homologous serum from patients receiving TUR, patients receiving cryosurgery, while also showing reduction in autologous serum, showed increased responsiveness when cultured in homologous serum. Although transient, depression of lymphocyte responsiveness, particularly if involving tumour-cloned T cells, may provide reduced surveillance to potential tumour cells leading to an alteration of tumour-host homeostasis. The potential of reduced tumour surveillance at least in the case of TUR, appears to be supported by observations that patients expiring from prostatic cancer at our institution had an antecedent TUR. The possibility of identifying those patients possessing aberrations of responsiveness prior to therapy, as well as those prone to develop or undergo further reduction in their responsiveness following the presently evaluated treatment modalities would appear to be of real and relevant concern in the management of the patient with prostatic, as well as other types of malignant neoplasms. The possibility of pre-operative and/or post-operative immunotherapy in such patients may be indicated pending further study.", "contents": "Evaluation of cellular immunologic responsiveness in the clinical management of patients with prostatic cancer. II. Effect of oestrogen, cryosurgery and transurethral resection on thymic-dependent lymphocytic blastogenesis. The effect of oestrogen, cryosurgery and transurethral resection (TUR) of the prostate on the blastogenic response of thymic-dependent peripheral blood lymphocytes (PBL) to the non-specific mitogen, phytohaemagglutinin (PHA) was evaluated as one in vitro criteria of each of these treatment modalities on the cellular immunologic responsiveness of 24 patients with prostatic cancer. A depression 5 days following receipt of oestrogen and 2-7 days following cryosurgery or TUR of the responsiveness of PHA-stimulated PBL was observed. Oestrogen-induced aberrations of responsiveness may not only be of relevance in prostatic cancer patients, but also suggested association between uterine cancer and prolonged administration of diethylstilboesterol and the development of vaginal tumours in offspring found in association with maternal ingestion during pregnancy. Particularly striking was that contrary to the reduced responsiveness of PBL cultured in autologous and homologous serum from patients receiving TUR, patients receiving cryosurgery, while also showing reduction in autologous serum, showed increased responsiveness when cultured in homologous serum. Although transient, depression of lymphocyte responsiveness, particularly if involving tumour-cloned T cells, may provide reduced surveillance to potential tumour cells leading to an alteration of tumour-host homeostasis. The potential of reduced tumour surveillance at least in the case of TUR, appears to be supported by observations that patients expiring from prostatic cancer at our institution had an antecedent TUR. The possibility of identifying those patients possessing aberrations of responsiveness prior to therapy, as well as those prone to develop or undergo further reduction in their responsiveness following the presently evaluated treatment modalities would appear to be of real and relevant concern in the management of the patient with prostatic, as well as other types of malignant neoplasms. The possibility of pre-operative and/or post-operative immunotherapy in such patients may be indicated pending further study."}
{"id": "PMID:1087496", "title": "[Necrosis of the prostate and the urinary bladder during kumarin therapy (author's transl)].", "content": "The very first case of part necrosis of the ruinary bladder and prostate during therapy with kumarin is presented. The pathogenesis of kumarin induced necrosis is discussed. Apart from other findings there was histologically proved thrombosis of arteries and veins.", "contents": "[Necrosis of the prostate and the urinary bladder during kumarin therapy (author's transl)]. The very first case of part necrosis of the ruinary bladder and prostate during therapy with kumarin is presented. The pathogenesis of kumarin induced necrosis is discussed. Apart from other findings there was histologically proved thrombosis of arteries and veins."}
{"id": "PMID:1087491", "title": "Fatal cerebral embolism following aorto-coronary bypass graft surgery.", "content": "In a case of fatal cerebral embolic infarction following aorto-coronary bypass graft (ACBG) surgery, postmortem examination revealed thrombosis of the vein grafts to the left circumflex and left anterior descending coronary arteries. Continguous with the thrombus in the graft to the circumflex artery was thrombotic material adherent to the aortic sutures and extending several millimeters into the lumen of the aorta. A nonadherent thrombus of similar histologic character was found in the right middle cerebral artery, associated with localized brain infarction. In addition to the risks of cerebral complication associated with other types of open-heart surgery, the location of the vein grafts in patients undergoing ACBG operations seems to offer a unique mechansim for the occurrence of systemic and cerebral embolism, which may be operational in other cases.", "contents": "Fatal cerebral embolism following aorto-coronary bypass graft surgery. In a case of fatal cerebral embolic infarction following aorto-coronary bypass graft (ACBG) surgery, postmortem examination revealed thrombosis of the vein grafts to the left circumflex and left anterior descending coronary arteries. Continguous with the thrombus in the graft to the circumflex artery was thrombotic material adherent to the aortic sutures and extending several millimeters into the lumen of the aorta. A nonadherent thrombus of similar histologic character was found in the right middle cerebral artery, associated with localized brain infarction. In addition to the risks of cerebral complication associated with other types of open-heart surgery, the location of the vein grafts in patients undergoing ACBG operations seems to offer a unique mechansim for the occurrence of systemic and cerebral embolism, which may be operational in other cases."}
{"id": "PMID:1087502", "title": "Complement levles in relation to certain antibodies in polytransfused thalassaemic patients.", "content": "Polytransfused patients are exposed to a variety of antigenic stimuli which may eventually trigger autoimmune reactions. The relation of serum complement to the occurrence of various antibodies has been investigated in a group of 75 polytransfused thalassaemic patients. The incidence of the antibodies studied was as follows: anti-HBs 77%, anti-Gm 64%, rheumatoid factor 31%, antinuclear 13%, anti-smooth muscle 47%. Anti-perietal cell, anti-thyroid and anti-kidney were only rarely found and antimitochondrial antibodies were never detected. Total hemolytic complement was low in 6 and incidentally decreased in 21 patients. A positive correlation was found between low serum complement and presence of rheumatoid factor. The high incidence of anti-smooth muscle antibodies seems to be unrelated to liver disease.", "contents": "Complement levles in relation to certain antibodies in polytransfused thalassaemic patients. Polytransfused patients are exposed to a variety of antigenic stimuli which may eventually trigger autoimmune reactions. The relation of serum complement to the occurrence of various antibodies has been investigated in a group of 75 polytransfused thalassaemic patients. The incidence of the antibodies studied was as follows: anti-HBs 77%, anti-Gm 64%, rheumatoid factor 31%, antinuclear 13%, anti-smooth muscle 47%. Anti-perietal cell, anti-thyroid and anti-kidney were only rarely found and antimitochondrial antibodies were never detected. Total hemolytic complement was low in 6 and incidentally decreased in 21 patients. A positive correlation was found between low serum complement and presence of rheumatoid factor. The high incidence of anti-smooth muscle antibodies seems to be unrelated to liver disease."}
{"id": "PMID:1087503", "title": "False-positive Rose-Waaler rheumatoid factor titre given by anti-ruminant IgM antibody.", "content": "An antibody against ruminant (goat, sheep, bovine) IgM is described which also cross-reacts with rabbit IgM. The antibody was found in the serum of a patient with adult coeliac disease and a selective IgA deficiency. This patient gave a false high Rose-Waaler rheumatoid factor titre due to the presence of the serum antibody to rabbit IgM that was capable of agglutinating rabbit antibody-sensitised erythrocytes.", "contents": "False-positive Rose-Waaler rheumatoid factor titre given by anti-ruminant IgM antibody. An antibody against ruminant (goat, sheep, bovine) IgM is described which also cross-reacts with rabbit IgM. The antibody was found in the serum of a patient with adult coeliac disease and a selective IgA deficiency. This patient gave a false high Rose-Waaler rheumatoid factor titre due to the presence of the serum antibody to rabbit IgM that was capable of agglutinating rabbit antibody-sensitised erythrocytes."}
{"id": "PMID:1087506", "title": "[T-Lymphocyte shifts in patients with melanoma and bronchogenic carcinoma (author's transl)].", "content": "Lymphocyte subpopulations were investigated in 32 patients with malignant melanoma, 25 patients with bronchogenic carcinoma and 59 control subjects. Whereas the determination of membrane immunoglobulin-bearing lymphocytes and of complement receptor-bearing lymphocytes gave comparable results in the tumour patient and control group, significant differences were found in the T-lymphocyte population using the sheep red blood cell (SRBC) assay. The so-called \"active\" Wybran rosette test, which characterizes a  T-cell subpopulation with an especially avid receptor for SRBC, gave significantly lower results in patients with melanoma (25% control 35%, p less than 0.01) and bronchogenic carcinoma (21% control 35% p less than 0.01). Determination of the total T-cell population using the Jondal rosette assay gave significantly lower values in the patients with melanoma (52%, control 63%, p less than 0.01), but not in those with lung cancer (65%). Low rosette values were detected even at low histological invasion levels (classified according to Clark) in the patients with melanoma. No correlation was found between the invasion level and the percentage of rosette-forming cells. The significance of these findings and the value of the rosette assay in the assessment of the immunological reactivity of tumour patients is discussed.", "contents": "[T-Lymphocyte shifts in patients with melanoma and bronchogenic carcinoma (author's transl)]. Lymphocyte subpopulations were investigated in 32 patients with malignant melanoma, 25 patients with bronchogenic carcinoma and 59 control subjects. Whereas the determination of membrane immunoglobulin-bearing lymphocytes and of complement receptor-bearing lymphocytes gave comparable results in the tumour patient and control group, significant differences were found in the T-lymphocyte population using the sheep red blood cell (SRBC) assay. The so-called \"active\" Wybran rosette test, which characterizes a  T-cell subpopulation with an especially avid receptor for SRBC, gave significantly lower results in patients with melanoma (25% control 35%, p less than 0.01) and bronchogenic carcinoma (21% control 35% p less than 0.01). Determination of the total T-cell population using the Jondal rosette assay gave significantly lower values in the patients with melanoma (52%, control 63%, p less than 0.01), but not in those with lung cancer (65%). Low rosette values were detected even at low histological invasion levels (classified according to Clark) in the patients with melanoma. No correlation was found between the invasion level and the percentage of rosette-forming cells. The significance of these findings and the value of the rosette assay in the assessment of the immunological reactivity of tumour patients is discussed."}
{"id": "PMID:1087514", "title": "Alpha-galactosyltransferase activity in the serum of frogs (Rana catesbeiana).", "content": "Human blood group 0 cells were converted into B-active cells by incubation with uridine diphosphate D-galactose and unfractionated serum from bull frog (Rana catesbeiana). Under these conditions, \"Bombay\" (Oh) type red cells were not converted, and group 0 foetal cells possessing a few H antigenic sites were only weakly converted into B-active cells. In addition, the agglutination titer of the converted cells with anti-H serum decreased. These results therefore indicate that the conversion depends upon the presence of H-substance on the red cells. It was concluded from the results of this investigation that the blood group B specific alpha-galactosyltransferase was present in the serum of Rana catesbeiana as well as in the human group B serum.", "contents": "Alpha-galactosyltransferase activity in the serum of frogs (Rana catesbeiana). Human blood group 0 cells were converted into B-active cells by incubation with uridine diphosphate D-galactose and unfractionated serum from bull frog (Rana catesbeiana). Under these conditions, \"Bombay\" (Oh) type red cells were not converted, and group 0 foetal cells possessing a few H antigenic sites were only weakly converted into B-active cells. In addition, the agglutination titer of the converted cells with anti-H serum decreased. These results therefore indicate that the conversion depends upon the presence of H-substance on the red cells. It was concluded from the results of this investigation that the blood group B specific alpha-galactosyltransferase was present in the serum of Rana catesbeiana as well as in the human group B serum."}
{"id": "PMID:1087521", "title": "[Bleeding from hiatal hernia (author's transl)].", "content": "Report on 7 cases of massive hemorrhage from hiatal hernias. In all cases diagnosis was made before operation by oesophago-gastroscopy and/or by selective angiography. Early operation is the only successful therapy. The procedure depends on the kind of lesion and the type of hernia. In sliding hernia a Nissen fundoplication, in the paraesophageal type a fundoplication combined with gastropexy is performed. In cases of hyperacidity a combination with vagotomy and pylorplasty is necessary. Ulcers should be excised or resected by a Billroth-I-type gastrectomy. All seven patients could be cured.", "contents": "[Bleeding from hiatal hernia (author's transl)]. Report on 7 cases of massive hemorrhage from hiatal hernias. In all cases diagnosis was made before operation by oesophago-gastroscopy and/or by selective angiography. Early operation is the only successful therapy. The procedure depends on the kind of lesion and the type of hernia. In sliding hernia a Nissen fundoplication, in the paraesophageal type a fundoplication combined with gastropexy is performed. In cases of hyperacidity a combination with vagotomy and pylorplasty is necessary. Ulcers should be excised or resected by a Billroth-I-type gastrectomy. All seven patients could be cured."}
{"id": "PMID:1087522", "title": "[Portosystemic anastomosis during pregnancy].", "content": "There is discussed the surgical treatment of massive otherwise uncontrolable haemorrhage from oesophageal varices during advanced pregnancy. The authors have performed in the above situation a splenorenal shunt in a women suffering from the prehepatic portal hypertension during her sixth month of pregnancy. The pregnancy was maintained but an unviable child was born. The authors were able to find only 5 quotations from the references available to them dealing with 5 similar cases in women who delivered healthy babies thanks to a surgical intervention (5 times a portal systemic shunt, once a direct operation). The most important prevention of those exceptional surgical indications is the careful evaluation of all circumstances for a pregnancy in women with portal hypertension.", "contents": "[Portosystemic anastomosis during pregnancy]. There is discussed the surgical treatment of massive otherwise uncontrolable haemorrhage from oesophageal varices during advanced pregnancy. The authors have performed in the above situation a splenorenal shunt in a women suffering from the prehepatic portal hypertension during her sixth month of pregnancy. The pregnancy was maintained but an unviable child was born. The authors were able to find only 5 quotations from the references available to them dealing with 5 similar cases in women who delivered healthy babies thanks to a surgical intervention (5 times a portal systemic shunt, once a direct operation). The most important prevention of those exceptional surgical indications is the careful evaluation of all circumstances for a pregnancy in women with portal hypertension."}
{"id": "PMID:1087526", "title": "Tumour-host relationship and its implications in the treatment and long-term prognosis of laryngeal cancer. Critical observations on the TNM system of tumour classification.", "content": "The choice of surgical and/or X-ray treatment of a laryngeal cancer is usually established by taking into consideration the site of the tumor and its extension. The histologic type is considered only when this is easily identifiable, whereas the histologic grade of cellular differentiation and the tumour-host relationship are always erroneously disregarded. These latter factors are of a basic importance in establishing a \"tailor-made\" therapeutic programme for each patient and the long-term prognosis. The above factors have led to a new critical approach to laryngeal cancer and have also made it possible to obtain some practical results: 1 degrees the correct definition of the verrucous squamous cell carcinoma of the larynx, which is the result of the highest local defensive reaction to tumour growth (mediated by thymus-dependent lymphocytes); 2 degrees a criticism of the present trend to perform a more conservative surgery decided only opon the basis of the site and extension of the neoplasm; conservation surgery must be adopted for patients with an intense cellular immune response around the tumour and with a moderate histologic grade of malignancy; 3 degrees a criticism of the present criteria in establishing the long-term survival, which do not include the early immune response; 4 degrees the necessity of a systematic histologic study of the surgical specimen so as to evaluate the intensity of the immune reaction both around the tumour and in regional lymph nodes. In the light of these new data, the TNM system of tumour classification is now outdated, as witnessed by the constant efforts to adapt it to the unpredictable behaviour of malignant disease.", "contents": "Tumour-host relationship and its implications in the treatment and long-term prognosis of laryngeal cancer. Critical observations on the TNM system of tumour classification. The choice of surgical and/or X-ray treatment of a laryngeal cancer is usually established by taking into consideration the site of the tumor and its extension. The histologic type is considered only when this is easily identifiable, whereas the histologic grade of cellular differentiation and the tumour-host relationship are always erroneously disregarded. These latter factors are of a basic importance in establishing a \"tailor-made\" therapeutic programme for each patient and the long-term prognosis. The above factors have led to a new critical approach to laryngeal cancer and have also made it possible to obtain some practical results: 1 degrees the correct definition of the verrucous squamous cell carcinoma of the larynx, which is the result of the highest local defensive reaction to tumour growth (mediated by thymus-dependent lymphocytes); 2 degrees a criticism of the present trend to perform a more conservative surgery decided only opon the basis of the site and extension of the neoplasm; conservation surgery must be adopted for patients with an intense cellular immune response around the tumour and with a moderate histologic grade of malignancy; 3 degrees a criticism of the present criteria in establishing the long-term survival, which do not include the early immune response; 4 degrees the necessity of a systematic histologic study of the surgical specimen so as to evaluate the intensity of the immune reaction both around the tumour and in regional lymph nodes. In the light of these new data, the TNM system of tumour classification is now outdated, as witnessed by the constant efforts to adapt it to the unpredictable behaviour of malignant disease."}
{"id": "PMID:1087531", "title": "Intrathoracic pressure as a tourniquet technic to tamponade bleeding esophageal varices.", "content": "The authors' report observations on 79 cases of esophagovariceal bleeding in which ten cases were subjected to an intrathoracic tourniquet maneuver of increasing intrathoracic pressure. All esophagovariceal bleeding ceased following the maneuver. An established maneuver safety limit (holding mean transmural CVP to no higher than 4.0 cm H2O) insured cardiac output maintenance and guaranteed against maneuver-generated morbidity. The authors conclude the cessation of the bleeding from esophageal varices observed is the combined result of tourniquet-tamponade by direct venous air compression on the bleeding varices as well as general esophageal compression.", "contents": "Intrathoracic pressure as a tourniquet technic to tamponade bleeding esophageal varices. The authors' report observations on 79 cases of esophagovariceal bleeding in which ten cases were subjected to an intrathoracic tourniquet maneuver of increasing intrathoracic pressure. All esophagovariceal bleeding ceased following the maneuver. An established maneuver safety limit (holding mean transmural CVP to no higher than 4.0 cm H2O) insured cardiac output maintenance and guaranteed against maneuver-generated morbidity. The authors conclude the cessation of the bleeding from esophageal varices observed is the combined result of tourniquet-tamponade by direct venous air compression on the bleeding varices as well as general esophageal compression."}
{"id": "PMID:1087532", "title": "Metastases to the esophagus causing gastrointestinal bleeding.", "content": "A case of hypernephroma metastatic to the esophagus and presenting with massive upper gastrointestinal hemorrhage is described. The literature on metastatic esophageal neoplasm is reviewed and the methods of spread and prognosis summarized.", "contents": "Metastases to the esophagus causing gastrointestinal bleeding. A case of hypernephroma metastatic to the esophagus and presenting with massive upper gastrointestinal hemorrhage is described. The literature on metastatic esophageal neoplasm is reviewed and the methods of spread and prognosis summarized."}
{"id": "PMID:1087533", "title": "Alteration of colony-stimulating factor output, endotoxemia, and granulopoiesis in cyclic neutropenia.", "content": "Cellular and humoral factors involved in the regulation of granulopoiesis were evaluated in two patients with cyclic neutropenia by utilizing the agar-gel marrow culture technique to serially study marrow granulocytic colony-forming capacity (CFC) and the urinary output of colony-stimulating factor (CSF). CSF output varied inversely with peripheral neutrophil counts and directly with monocyte counts and evidence for infection (endotoxemia and/or staphylococcal abscesses). Following autologous infusion of one patient's plasma obtained during a period of neutropenia, increased urinary excretion of CSF occurred concomitant with increments in both marrow CFC and the proportion of granulocytic progenitor cells in DNA synthesis. Neutrophil periodicity was not altered by the administration of the neutropenic plasma. These findings are consistent with the hypothesis that cyclic neutropenia is caused by a quantitatively decreased entry of stem cells or granulocytic progenitor cells into granulopoiesis.", "contents": "Alteration of colony-stimulating factor output, endotoxemia, and granulopoiesis in cyclic neutropenia. Cellular and humoral factors involved in the regulation of granulopoiesis were evaluated in two patients with cyclic neutropenia by utilizing the agar-gel marrow culture technique to serially study marrow granulocytic colony-forming capacity (CFC) and the urinary output of colony-stimulating factor (CSF). CSF output varied inversely with peripheral neutrophil counts and directly with monocyte counts and evidence for infection (endotoxemia and/or staphylococcal abscesses). Following autologous infusion of one patient's plasma obtained during a period of neutropenia, increased urinary excretion of CSF occurred concomitant with increments in both marrow CFC and the proportion of granulocytic progenitor cells in DNA synthesis. Neutrophil periodicity was not altered by the administration of the neutropenic plasma. These findings are consistent with the hypothesis that cyclic neutropenia is caused by a quantitatively decreased entry of stem cells or granulocytic progenitor cells into granulopoiesis."}
{"id": "PMID:1087534", "title": "The myeloproliferative disorders. Correlation between clinical evolution and alterations of granulopoiesis.", "content": "Patients with myeloproliferative disorders were prospectively studied by in vitro agar-gel marrow culture technics to evaluate factors involved in the evolution of abnormal granulopoiesis. Marrow granulocytic colony-forming capacity was determined in 78 patients with chronic myeloid leukemia, subacute myeloid leukemia, preleukemia, Di Guglielmo's syndrome, polycythemia vera or essential thrombocythemia. A wide range of marrow colony-forming capacity values was noted early in disease courses; however, in 26 of 33 patients decreased colony-forming capacity was associated with disease transformation into acute myeloid leukemia or other clinically aggressive stages. An increased proportion of abnormally light buoyant density (less than 1.062 g/cm3) colony-forming cells was present in the marrow and peripheral blood of 15 of 16 patients with chronic myeloid leukemia, subacute myeloid leukemia, preleukemia or essential thrombocythemia; in seven of eight patients with greater than 35 per cent abnormally light colony-forming cells their disease subsequently underwent transformation. Elevated levels of urinary colony-stimulating factor output were noted in 17 of 31 patients, and in 10 of 12 patients whose disease subsequently underwent acute transformation within 10 months of study. In six of seven patients who simultaneously had an increased urinary output of colony-stimulating factor and low colony-forming capacity in marrow, transformation occurred within 10 months. These findings indicate that progressive abnormalities of both marrow clonal growth patterns and levels of possible humoral regulatory substances develop during evolution of these diseases. In contrast, patients with idiopathic sideroblastic ineffective erythropoiesis had normal values for marrow colony-forming capacity, proportion of light density colony-forming cells and urinary colony-stimulating factor output, and in none has their disease transformed into acute myeloid leukemia. These in vitro studies appear useful for clinical staging, evaluating prognosis and categorizing patients with myeloproliferative disorders.", "contents": "The myeloproliferative disorders. Correlation between clinical evolution and alterations of granulopoiesis. Patients with myeloproliferative disorders were prospectively studied by in vitro agar-gel marrow culture technics to evaluate factors involved in the evolution of abnormal granulopoiesis. Marrow granulocytic colony-forming capacity was determined in 78 patients with chronic myeloid leukemia, subacute myeloid leukemia, preleukemia, Di Guglielmo's syndrome, polycythemia vera or essential thrombocythemia. A wide range of marrow colony-forming capacity values was noted early in disease courses; however, in 26 of 33 patients decreased colony-forming capacity was associated with disease transformation into acute myeloid leukemia or other clinically aggressive stages. An increased proportion of abnormally light buoyant density (less than 1.062 g/cm3) colony-forming cells was present in the marrow and peripheral blood of 15 of 16 patients with chronic myeloid leukemia, subacute myeloid leukemia, preleukemia or essential thrombocythemia; in seven of eight patients with greater than 35 per cent abnormally light colony-forming cells their disease subsequently underwent transformation. Elevated levels of urinary colony-stimulating factor output were noted in 17 of 31 patients, and in 10 of 12 patients whose disease subsequently underwent acute transformation within 10 months of study. In six of seven patients who simultaneously had an increased urinary output of colony-stimulating factor and low colony-forming capacity in marrow, transformation occurred within 10 months. These findings indicate that progressive abnormalities of both marrow clonal growth patterns and levels of possible humoral regulatory substances develop during evolution of these diseases. In contrast, patients with idiopathic sideroblastic ineffective erythropoiesis had normal values for marrow colony-forming capacity, proportion of light density colony-forming cells and urinary colony-stimulating factor output, and in none has their disease transformed into acute myeloid leukemia. These in vitro studies appear useful for clinical staging, evaluating prognosis and categorizing patients with myeloproliferative disorders."}
{"id": "PMID:1087535", "title": "Ionic pathways of secretory membrane of frog gastric mucosa in Cl--free media.", "content": "Experiments were performed to determine the return ionic pathways of the secretory membrane of frog gastric mucosa associated with the electrogenic H+ pump in Cl--free media. The replacement of a 52 mM Na2SO4 secretory solution with a 52 mM K2SO4 secretory solution led to a decrease of resistance, an increase of the H+ secretory rate, and a reversal of the PD. The replacement of 52 mM Na2SO4 with 52 mM MgSO4 led to an increase of resistance and a decrease of the H+ rate. These results provided evidence for the existence of K+ and Na+ pathways, the former having a lower resistance than the latter. Short-circuiting the mucosa in Mg2+ solutions increased the H+ rate to the value in Na+ solutions, hence ruling out the possibility that Mg2+ might interfere with the H+ pump. The fact that the Mg2+ secretory solution, without K+ or Na+, did not abolish the H+ rate suggested the presence of at least a third ionic pathway. presumably SO42-, providing that Mg2+ does not penetrate the mucosa.", "contents": "Ionic pathways of secretory membrane of frog gastric mucosa in Cl--free media. Experiments were performed to determine the return ionic pathways of the secretory membrane of frog gastric mucosa associated with the electrogenic H+ pump in Cl--free media. The replacement of a 52 mM Na2SO4 secretory solution with a 52 mM K2SO4 secretory solution led to a decrease of resistance, an increase of the H+ secretory rate, and a reversal of the PD. The replacement of 52 mM Na2SO4 with 52 mM MgSO4 led to an increase of resistance and a decrease of the H+ rate. These results provided evidence for the existence of K+ and Na+ pathways, the former having a lower resistance than the latter. Short-circuiting the mucosa in Mg2+ solutions increased the H+ rate to the value in Na+ solutions, hence ruling out the possibility that Mg2+ might interfere with the H+ pump. The fact that the Mg2+ secretory solution, without K+ or Na+, did not abolish the H+ rate suggested the presence of at least a third ionic pathway. presumably SO42-, providing that Mg2+ does not penetrate the mucosa."}
{"id": "PMID:1087536", "title": "Transepithelial transport kinetics and Na entry in frog skin: effects of novobiocin.", "content": "Na+ entry across the outer surface of frog skin and transepithelial Na transport were studied simultaneously at different [Na] in either the presence or absence of novobiocin by direct measurements of J12 (unidirectional uptake) and Io (short-circuit current). J12 consisted of two components: one linear, the other saturable. The kinetic parameters of the saturating components in controls were close to the kinetic parameters of overall transepithelial transport (Jm12 = 1.68+/-0.13 mleq cm-2h-1; Io =1.80+/-0.14 mueq cm-2h-1. K12 = 6.02+/-1.27 mM;Kio=6.12+/-1.33 mM). Novobiocin significantly augmented net transepithelial Na transport by increasing J13. J31 remained unaffected. A 1:1 relationship between the saturating component of J12 and Io was observed in both treated and untreated skins at all [Na] tested. (Jm12Iom, k12, and Kio were significantly larger in treated skins, but despite very drastic changes in transport rates, a close correlation between kinetic parameters of entry step and transepithelial transport was maintained. This suggests that the kinetics of transepithelial transport may simply reflect those of the rate-limiting step: the Na entry across the outer barrier of the skin. The results indicate that the linear component of J12 is not involved in transepithelial transport kinetics.", "contents": "Transepithelial transport kinetics and Na entry in frog skin: effects of novobiocin. Na+ entry across the outer surface of frog skin and transepithelial Na transport were studied simultaneously at different [Na] in either the presence or absence of novobiocin by direct measurements of J12 (unidirectional uptake) and Io (short-circuit current). J12 consisted of two components: one linear, the other saturable. The kinetic parameters of the saturating components in controls were close to the kinetic parameters of overall transepithelial transport (Jm12 = 1.68+/-0.13 mleq cm-2h-1; Io =1.80+/-0.14 mueq cm-2h-1. K12 = 6.02+/-1.27 mM;Kio=6.12+/-1.33 mM). Novobiocin significantly augmented net transepithelial Na transport by increasing J13. J31 remained unaffected. A 1:1 relationship between the saturating component of J12 and Io was observed in both treated and untreated skins at all [Na] tested. (Jm12Iom, k12, and Kio were significantly larger in treated skins, but despite very drastic changes in transport rates, a close correlation between kinetic parameters of entry step and transepithelial transport was maintained. This suggests that the kinetics of transepithelial transport may simply reflect those of the rate-limiting step: the Na entry across the outer barrier of the skin. The results indicate that the linear component of J12 is not involved in transepithelial transport kinetics."}
{"id": "PMID:1087538", "title": "Quantitative determination of high molecular weight serum proteinase inhibitors in human semen.", "content": "Immunochemical determinations of serum proteinase inhibitors in human semen showed the presence of alpha1-antitrypsin and alpha1,x-antichymotrypsin, whereas inter-alpha-trypsin inhibitor, antithrombin III, alpha2-neuramino-glycoprotein and alpha2-macroglobulin could not be detected. Both serum proteinase inhibitors were determined in the seminal vesicle secretions of two patients with prostatic cancer. Employing the Ouchterlony double immunodiffusion technique pattern of identity was found between alpha1-antitrypsin resp. alpha1,x-antichymotrypsin in seminal plasma, seminal vesicle secretions and serum. Mean alpha1-antitrypsin concentration in seminal plasma of 129 andrological patients was 97.7 mug/ml and that of alpha1,x-antichymotrypsin 32.8 mug/ml. There were no differences in the mean alpha1-antitrypsin concentrations of normozoospermic and oligozoospermic ejaculates and those with seminal plasma fructose deficiency. Azoospermic ejaculates, however, showed a significant decrease of the mean alpha1-antitrypsin concentration (p less than 0.05). Alpha1,x-antichymotrypsin concentrations of normozoospermic ejaculates were significantly higher compared to those of oligozoospermia and azoospermia (p less than 0.05). Alpha1,x-antichymotrypsin levels in semen samples were fructose deficiency were not different from those of the total ejaculate population. The cause and significance of the observed differences in the inhibitor concentrations within the different ejaculate types is not known. However, there are no indications for the involvement of both proteinase inhibitors in male reproductive processes.", "contents": "Quantitative determination of high molecular weight serum proteinase inhibitors in human semen. Immunochemical determinations of serum proteinase inhibitors in human semen showed the presence of alpha1-antitrypsin and alpha1,x-antichymotrypsin, whereas inter-alpha-trypsin inhibitor, antithrombin III, alpha2-neuramino-glycoprotein and alpha2-macroglobulin could not be detected. Both serum proteinase inhibitors were determined in the seminal vesicle secretions of two patients with prostatic cancer. Employing the Ouchterlony double immunodiffusion technique pattern of identity was found between alpha1-antitrypsin resp. alpha1,x-antichymotrypsin in seminal plasma, seminal vesicle secretions and serum. Mean alpha1-antitrypsin concentration in seminal plasma of 129 andrological patients was 97.7 mug/ml and that of alpha1,x-antichymotrypsin 32.8 mug/ml. There were no differences in the mean alpha1-antitrypsin concentrations of normozoospermic and oligozoospermic ejaculates and those with seminal plasma fructose deficiency. Azoospermic ejaculates, however, showed a significant decrease of the mean alpha1-antitrypsin concentration (p less than 0.05). Alpha1,x-antichymotrypsin concentrations of normozoospermic ejaculates were significantly higher compared to those of oligozoospermia and azoospermia (p less than 0.05). Alpha1,x-antichymotrypsin levels in semen samples were fructose deficiency were not different from those of the total ejaculate population. The cause and significance of the observed differences in the inhibitor concentrations within the different ejaculate types is not known. However, there are no indications for the involvement of both proteinase inhibitors in male reproductive processes."}
{"id": "PMID:1087539", "title": "The prevalence of alpha-antitrypsin heterozygotes (Pi MZ) in patients with obstructive pulmonary disease.", "content": "An increased incidence of intermediate deficiency of serum alpha1-antitrypsin resulting from Pi phenotype MZ has been reported in patients with chronic obstructive pulmonary disease (COPD) by some laboratories but not confirmed by others. Prevalence of Pi MZ was determined in patients with COPD among 502 subjects referred to a pulmonary function testing laboratory in a region with low concentrations of air pollutants. Control prevalences were obtained from 930 randomly selected subjects in the same community as well as from patients without COPD referred to the laboratory. Depending on criteria used to define COPD, 155 to 306 subjects had COPD. Pi MZ prevalence in subjects with COPD varied from 1.5 to 4 times the prevalence in the community control group and in the patients without COPD. This difference approached significance or was significant. Because Pi MZ was present in only 3.5 to 4.5 per cent of patients with COPD, Pi MZ is not a major factor in the etiology of COPD in this community. The higher incidence of Pi MZ inpatients with COPD reported by other investigators may be explained by small sample size, bias in selection of study or control population groups, or the development of COPD from interaction between Pi MZ and air pollutants or other factors not present in this community.", "contents": "The prevalence of alpha-antitrypsin heterozygotes (Pi MZ) in patients with obstructive pulmonary disease. An increased incidence of intermediate deficiency of serum alpha1-antitrypsin resulting from Pi phenotype MZ has been reported in patients with chronic obstructive pulmonary disease (COPD) by some laboratories but not confirmed by others. Prevalence of Pi MZ was determined in patients with COPD among 502 subjects referred to a pulmonary function testing laboratory in a region with low concentrations of air pollutants. Control prevalences were obtained from 930 randomly selected subjects in the same community as well as from patients without COPD referred to the laboratory. Depending on criteria used to define COPD, 155 to 306 subjects had COPD. Pi MZ prevalence in subjects with COPD varied from 1.5 to 4 times the prevalence in the community control group and in the patients without COPD. This difference approached significance or was significant. Because Pi MZ was present in only 3.5 to 4.5 per cent of patients with COPD, Pi MZ is not a major factor in the etiology of COPD in this community. The higher incidence of Pi MZ inpatients with COPD reported by other investigators may be explained by small sample size, bias in selection of study or control population groups, or the development of COPD from interaction between Pi MZ and air pollutants or other factors not present in this community."}
{"id": "PMID:1087540", "title": "Racial distribution of alpha1-antitrypsin variants among junior high school students.", "content": "A survey of alpha1-antitrypsin phenotypes and serum trypsin inhibitory capacities was performed in 1,841 seventh grade junior high school students. Antitrypsin deficiency states were detected only in white subjects (3.04) per cent) and not in 461 subjects of other races or of Mexican origin. A screening level of serum trypsin inhibitory capacity at 75 per cent of normal or less included all of the deficient phenotypes, but phenotyping was necessary to eliminate a small percentage of normal and unimportant variant phenotypes from this group. It is suggested that future programs for detecting antitrypsin deficiency and for counseling students who are genetically prone to develop pulmonary emphysema be aimed primarily at white populations.", "contents": "Racial distribution of alpha1-antitrypsin variants among junior high school students. A survey of alpha1-antitrypsin phenotypes and serum trypsin inhibitory capacities was performed in 1,841 seventh grade junior high school students. Antitrypsin deficiency states were detected only in white subjects (3.04) per cent) and not in 461 subjects of other races or of Mexican origin. A screening level of serum trypsin inhibitory capacity at 75 per cent of normal or less included all of the deficient phenotypes, but phenotyping was necessary to eliminate a small percentage of normal and unimportant variant phenotypes from this group. It is suggested that future programs for detecting antitrypsin deficiency and for counseling students who are genetically prone to develop pulmonary emphysema be aimed primarily at white populations."}
{"id": "PMID:1087529", "title": "Localization of T and B lymphocytes in human adenoid, tonsil, appendix and Peyer's patches.", "content": "T and B lymphocytes were detected in human adenoid, tonsil, appendix and Peyer's patches by adherence to sheep erythrocytes (E) and human erythrocytes sensitized with antibody and complement (HEAC). The percentages of T lymphocytes in adenoid and tonsil cell suspensions averaged 30.9 +/- 3.4 (S.D.) and 35.8 +/- 6.4 (S.D.). The percentages of B lymphocytes in the same tissues were 42.5 +/- 11.3 (S.D.) and 40.1 +/- 34 (S.D.) respectively. In adenoid and tonsil tissue sections, B lymphocytes were found in the follicles and T lymphocytes were detected around the follicular areas. The predominant cell population in Peyer's patches and appendix sections was constituted by B lymphocytes.", "contents": "Localization of T and B lymphocytes in human adenoid, tonsil, appendix and Peyer's patches. T and B lymphocytes were detected in human adenoid, tonsil, appendix and Peyer's patches by adherence to sheep erythrocytes (E) and human erythrocytes sensitized with antibody and complement (HEAC). The percentages of T lymphocytes in adenoid and tonsil cell suspensions averaged 30.9 +/- 3.4 (S.D.) and 35.8 +/- 6.4 (S.D.). The percentages of B lymphocytes in the same tissues were 42.5 +/- 11.3 (S.D.) and 40.1 +/- 34 (S.D.) respectively. In adenoid and tonsil tissue sections, B lymphocytes were found in the follicles and T lymphocytes were detected around the follicular areas. The predominant cell population in Peyer's patches and appendix sections was constituted by B lymphocytes."}
{"id": "PMID:1087530", "title": "Clinical and immunological improvement in a patient with chronic mucocutaneous candidiasis treated with transfer factor.", "content": "In a 19 year old patient suffering from CMC since the first months of life, clinical improvement accompanied by correction of the immunologic defect was achieved by Transfer Factor therapy. After 12 months from the last administration of Transfer Factor the improvement persisted. The positive outcome of the treatment in this disease is not constant. Possibly only patients with cellular immunologic defects are susceptible of a favourable response, moreover it is thinkable that the quality of Transfer Factor and the dosage administered must play a role.", "contents": "Clinical and immunological improvement in a patient with chronic mucocutaneous candidiasis treated with transfer factor. In a 19 year old patient suffering from CMC since the first months of life, clinical improvement accompanied by correction of the immunologic defect was achieved by Transfer Factor therapy. After 12 months from the last administration of Transfer Factor the improvement persisted. The positive outcome of the treatment in this disease is not constant. Possibly only patients with cellular immunologic defects are susceptible of a favourable response, moreover it is thinkable that the quality of Transfer Factor and the dosage administered must play a role."}
{"id": "PMID:1087541", "title": "[Focal infection on O.R.L. and its distant aseptic manifestations].", "content": "In connection with the cases of a group of patients hospitalised in Departments of Infectious Pathology, the authors consider the various general and visceral symptoms caused by the focal infection. In these statistics, fever occupies the first place and in its isolated form poses difficult diagnostic problems which can only be solved by a process of elimination. Repeated biological tests and careful attention to the clinical symptoms are necessary to link what has been observed to an infection which, it should be stressed, is descrete. Articular lesion, very different from acute articular rheumatism, cutaneous, ocular and renal symptoms are each in turn discussed and analyzed. From their personal observations, the authors recall the possibility of serous lesions and, in particular, of recurrent pericarditis. A summing-up of the theories concerning prescription completes this clinical survey which ends by recalling the function of the oto-rhino-laryngologist in the search for the causal infection and the difficulties involved in ascribing responsibility to an almost invariably latent focal infection.", "contents": "[Focal infection on O.R.L. and its distant aseptic manifestations]. In connection with the cases of a group of patients hospitalised in Departments of Infectious Pathology, the authors consider the various general and visceral symptoms caused by the focal infection. In these statistics, fever occupies the first place and in its isolated form poses difficult diagnostic problems which can only be solved by a process of elimination. Repeated biological tests and careful attention to the clinical symptoms are necessary to link what has been observed to an infection which, it should be stressed, is descrete. Articular lesion, very different from acute articular rheumatism, cutaneous, ocular and renal symptoms are each in turn discussed and analyzed. From their personal observations, the authors recall the possibility of serous lesions and, in particular, of recurrent pericarditis. A summing-up of the theories concerning prescription completes this clinical survey which ends by recalling the function of the oto-rhino-laryngologist in the search for the causal infection and the difficulties involved in ascribing responsibility to an almost invariably latent focal infection."}
{"id": "PMID:1087542", "title": "Thymus: primary site of lymphopoiesis.", "content": "The thymus of the leopard frog is the primary, if not the sole, source of progenitor lymphocytic cells that normally colonize the spleen. Following embryonal parabiosis, a high level of cell chimerism exists in the thymus glands of each member of the parabiotic pair. Evidently, there can be an appreciable amount of cross-circulation of thymic cells between thymus glands.", "contents": "Thymus: primary site of lymphopoiesis. The thymus of the leopard frog is the primary, if not the sole, source of progenitor lymphocytic cells that normally colonize the spleen. Following embryonal parabiosis, a high level of cell chimerism exists in the thymus glands of each member of the parabiotic pair. Evidently, there can be an appreciable amount of cross-circulation of thymic cells between thymus glands."}
{"id": "PMID:1087543", "title": "Alternate sites of lymphopoiesis in the amphibian embryo.", "content": "The presumptive ventral blood islands were reciprocally transplanted between chromosomally labelled 72-hour-old leopard frog embryos. At 10 days of development, the differentiating epithelial thymus glands were surgically removed. Following metamorphosis, the spleen and bone marrow from juvenile frogs were analysed cytophotometrically. During normal ontogeny, the vast majority of peripheral lymphocytes are derived from the thymus, not the ventral blood islands. In the absence of the thymus, the ventral blood islands made the major lymphocyte contribution to the peripheral lymphoid organs. Thus, removal of the thymus appears to initiate an alternate pathway of lymphocyte differentiation in the leopard frog.", "contents": "Alternate sites of lymphopoiesis in the amphibian embryo. The presumptive ventral blood islands were reciprocally transplanted between chromosomally labelled 72-hour-old leopard frog embryos. At 10 days of development, the differentiating epithelial thymus glands were surgically removed. Following metamorphosis, the spleen and bone marrow from juvenile frogs were analysed cytophotometrically. During normal ontogeny, the vast majority of peripheral lymphocytes are derived from the thymus, not the ventral blood islands. In the absence of the thymus, the ventral blood islands made the major lymphocyte contribution to the peripheral lymphoid organs. Thus, removal of the thymus appears to initiate an alternate pathway of lymphocyte differentiation in the leopard frog."}
{"id": "PMID:1087549", "title": "Extrahepatic biliary atresia versus neonatal hepatitis. Review of 137 prospectively investigated infants.", "content": "In a prospective regional survey of neonatal hepatitis syndrome 32 infants had extrahepatic biliary atresia (EHBA) and 103 had hepatitis. No cause for the lesion was found in infants with extrahepatic biliary atresia, but in 32 with hepatitis a specific cause was identified, 24 having genetic deficiency of the serum protein alpha1-antitrypsin. No differences were observed in parental age, mother's health in pregnancy, month of birth, birth order, or sex of the infants. Familial idiopathic hepatitis occurred in 3 of 67 sibs of patients with idiopathic hepatitis, but the 33 sibs of EHBA patients had no liver disease. Of the infants with hepatitis, 36 were of low birthweight, less than 2.5 kg, and 23 were born prematurely. Infants with biliary atresia were all of normal birthweight and only one was born prematurely. Consideration of clinical and biochemical abnormalities in the first 2 months of life showed no differences between the two groups except that infants with EHBA were more commonly jaundiced from birth (80%) and had more frequently acholic stools (83%). The frequency of these features in patients with hepatitis being 68% and 52%. Standard tests of liver function were not discriminatory. Percutaneous liver biopsies were diagnostic in 75% of those with EHBA and in 92% of those with hepatitis. The I131 Rose Bengal faecal excretion was less than 10% in 26 of 28 infants with EHBA and in only 5 of 18 with hepatitis. These latter two investigations together allowed a correct preoperativer diagnosis of EHBA in all instances. Bile drainage was achieved surgically in only 3 cases. A major reason for these poor results may have been the late referral of cases for diagnosis and laparotomy, which should be performed as soon as the diagnosis is suspected and always by 70 days of age.", "contents": "Extrahepatic biliary atresia versus neonatal hepatitis. Review of 137 prospectively investigated infants. In a prospective regional survey of neonatal hepatitis syndrome 32 infants had extrahepatic biliary atresia (EHBA) and 103 had hepatitis. No cause for the lesion was found in infants with extrahepatic biliary atresia, but in 32 with hepatitis a specific cause was identified, 24 having genetic deficiency of the serum protein alpha1-antitrypsin. No differences were observed in parental age, mother's health in pregnancy, month of birth, birth order, or sex of the infants. Familial idiopathic hepatitis occurred in 3 of 67 sibs of patients with idiopathic hepatitis, but the 33 sibs of EHBA patients had no liver disease. Of the infants with hepatitis, 36 were of low birthweight, less than 2.5 kg, and 23 were born prematurely. Infants with biliary atresia were all of normal birthweight and only one was born prematurely. Consideration of clinical and biochemical abnormalities in the first 2 months of life showed no differences between the two groups except that infants with EHBA were more commonly jaundiced from birth (80%) and had more frequently acholic stools (83%). The frequency of these features in patients with hepatitis being 68% and 52%. Standard tests of liver function were not discriminatory. Percutaneous liver biopsies were diagnostic in 75% of those with EHBA and in 92% of those with hepatitis. The I131 Rose Bengal faecal excretion was less than 10% in 26 of 28 infants with EHBA and in only 5 of 18 with hepatitis. These latter two investigations together allowed a correct preoperativer diagnosis of EHBA in all instances. Bile drainage was achieved surgically in only 3 cases. A major reason for these poor results may have been the late referral of cases for diagnosis and laparotomy, which should be performed as soon as the diagnosis is suspected and always by 70 days of age."}
{"id": "PMID:1087544", "title": "B-cell ontogeny in the chicken.", "content": "We conclude that our data are most consistent with the hypothesis that individual bursal stem cells give rise to multiple clones of B lymphocytes by a preprogrammed sequence of variable (V) region genes. The alternate hypothesis that each stem cell gives rise to one B cell expressing but one set of VH genes could only fit our data if the additional assumption is made that each stem cell is preprogrammed to give rise to an unique B-cell clonotype at a fixed time during development. Since we were unable to influence the pattern of expression of clonotypic diversity by modifying the exposure to antigens and since random somatic mutations would seem an inefficient mechanism for systematic generation of the diversity, we favor the possibility of a genetic mechanism involving an orderly pairing of germ-line VH and VL genes. The corollary of this hypothesis is that V-region diversity is generated first and Ig-class diversity is secondarily expressed within each clone by a switch mechanism for the sequential expression of CH genes.", "contents": "B-cell ontogeny in the chicken. We conclude that our data are most consistent with the hypothesis that individual bursal stem cells give rise to multiple clones of B lymphocytes by a preprogrammed sequence of variable (V) region genes. The alternate hypothesis that each stem cell gives rise to one B cell expressing but one set of VH genes could only fit our data if the additional assumption is made that each stem cell is preprogrammed to give rise to an unique B-cell clonotype at a fixed time during development. Since we were unable to influence the pattern of expression of clonotypic diversity by modifying the exposure to antigens and since random somatic mutations would seem an inefficient mechanism for systematic generation of the diversity, we favor the possibility of a genetic mechanism involving an orderly pairing of germ-line VH and VL genes. The corollary of this hypothesis is that V-region diversity is generated first and Ig-class diversity is secondarily expressed within each clone by a switch mechanism for the sequential expression of CH genes."}
{"id": "PMID:1087545", "title": "In vitro studies on the ontogeny of lymphocyte populations.", "content": "We have investigated the ontogency of lymphocyte populations in the mouse embryo using organ culture techniques. Our results indicate that B lymphocytes are generated independently in foetal liver, foetal spleen and foetal bone marrow, while T lymphocytes are generated in thymus. We have characterised the structural and functional properties of developing lymphocytes in the organs. Our results suggest that foetal lymphocytes may differ from adult lymphocytes in a number of important respects which may have a bearing on the generation of tolerance to self antigens.", "contents": "In vitro studies on the ontogeny of lymphocyte populations. We have investigated the ontogency of lymphocyte populations in the mouse embryo using organ culture techniques. Our results indicate that B lymphocytes are generated independently in foetal liver, foetal spleen and foetal bone marrow, while T lymphocytes are generated in thymus. We have characterised the structural and functional properties of developing lymphocytes in the organs. Our results suggest that foetal lymphocytes may differ from adult lymphocytes in a number of important respects which may have a bearing on the generation of tolerance to self antigens."}
{"id": "PMID:1087546", "title": "Ontogeny of thymus factor secretion.", "content": "The circulating thymic factor (TF) detected by 0-conversion assay has been followed up in ontogeny. Serum TF evaluation in pregnant thymectomized mice and in new born mice indicates that TF secretion starts on day 12-13 of foetal life and has reached the adult's level at birth.", "contents": "Ontogeny of thymus factor secretion. The circulating thymic factor (TF) detected by 0-conversion assay has been followed up in ontogeny. Serum TF evaluation in pregnant thymectomized mice and in new born mice indicates that TF secretion starts on day 12-13 of foetal life and has reached the adult's level at birth."}
{"id": "PMID:1087547", "title": "Maturation of thymus-dependent lymphocytes after confrontation with histo-incompatible cells.", "content": "Antigen-induced differentiation of mature T lymphocytes after in vitro exposure to transplantation antigens has been reviewed. Upon confrontation with histo-incompatible cells the resting alloantigen-reactive T lymphocytes transform into blasts and divide several times. Concomitantly they acquire the capacity to kill relevant allogeneic target cells. Generation of killer cells requires DNA synthesis and cell division. It is also evident that at least two subsets of T cells participate in the generation of T killer cells. In vivo studies suggest that the activated T cells cooperate with B cells, and that this cooperation is necessary in IgG class alloantibody response. Other studies, performed in vitro, have demonstrated that the alloantigen-activated lymphocytes may also have an immulogically specific suppressive effect on an alloimmune response. At any rate, after the cell proliferation ceases, the T blasts return to resting state and revert back into small lymphocytes. The \"secondary\" T memory lymphocytes are restricted to the antigens of the first confrontation. Second stimulation by the original histocompatible (but not by unrelated) cells leads to a characteristic prompt secondary proliferative and cytotoxic response. The memory T lymphocytes do not respond to the T mitogens (PHA and ConA) but do respond to relevant histo-incompatible membrane preparations and to relevant UV-killed stimulator cells (non-primed T lymphocytes are unalbe to do so). Cell division is not absolutely necessary for the generation of killer cells in the anamnestic secondary response. The memory T lymphocytes are long-lived and are recovered up to 20 weeks after parking to T-deprived syngeneic hosts. While resting in the syngeneic host they grandually lose the ability to respond promptly, indicating that T-cell memory on the cellular level is limited.", "contents": "Maturation of thymus-dependent lymphocytes after confrontation with histo-incompatible cells. Antigen-induced differentiation of mature T lymphocytes after in vitro exposure to transplantation antigens has been reviewed. Upon confrontation with histo-incompatible cells the resting alloantigen-reactive T lymphocytes transform into blasts and divide several times. Concomitantly they acquire the capacity to kill relevant allogeneic target cells. Generation of killer cells requires DNA synthesis and cell division. It is also evident that at least two subsets of T cells participate in the generation of T killer cells. In vivo studies suggest that the activated T cells cooperate with B cells, and that this cooperation is necessary in IgG class alloantibody response. Other studies, performed in vitro, have demonstrated that the alloantigen-activated lymphocytes may also have an immulogically specific suppressive effect on an alloimmune response. At any rate, after the cell proliferation ceases, the T blasts return to resting state and revert back into small lymphocytes. The \"secondary\" T memory lymphocytes are restricted to the antigens of the first confrontation. Second stimulation by the original histocompatible (but not by unrelated) cells leads to a characteristic prompt secondary proliferative and cytotoxic response. The memory T lymphocytes do not respond to the T mitogens (PHA and ConA) but do respond to relevant histo-incompatible membrane preparations and to relevant UV-killed stimulator cells (non-primed T lymphocytes are unalbe to do so). Cell division is not absolutely necessary for the generation of killer cells in the anamnestic secondary response. The memory T lymphocytes are long-lived and are recovered up to 20 weeks after parking to T-deprived syngeneic hosts. While resting in the syngeneic host they grandually lose the ability to respond promptly, indicating that T-cell memory on the cellular level is limited."}
{"id": "PMID:1087551", "title": "Selective concentration and localization of gold in macrophages of synovial and other tissues during and after chrysotherapy in rheumatoid patients.", "content": "Gold distribution was studied in the tissues of 7 rheumatoid patients who had died from 0 to 23 years after stopping chrysotherapy (sodium aurothiomalate) and in 23 samples of synovial tissue removed surgically at intervals during chrysotherapy in 5 patients. After the application of a highly specific staining technique, the cellular localization of gold was examined microscopically in various tissues: the amounts of gold in selected areas of the same specimens which had been examined microscopically were then measured by neutron activation analysis. During active chrysotherapy gold was abundant in synovial lining cells except where a fibrin layer was present on the surface; after stopping chrysotherapy, gold disappeared from the synovial lining cells. Gold accumulated progressively in the subsynovial connective tissues during chrysotherapy, but was not uniformly distributed, and bore no relationship to fluctuations in serum gold levels. Gold deposition was not confined to joint tissue, but was found within the macrophages of many organs, renal tubular epithelium, and, after recent chrysotherapy, in seminiferous tubules, hepatocytes, and adrenal cortical cells. Gold persisted in synovial and other tissues for up to 23 years after chrysotherapy was stopped. The overall findings indicated that gold is selectively concentrated within inflamed synovial tissues during chrysotherapy.", "contents": "Selective concentration and localization of gold in macrophages of synovial and other tissues during and after chrysotherapy in rheumatoid patients. Gold distribution was studied in the tissues of 7 rheumatoid patients who had died from 0 to 23 years after stopping chrysotherapy (sodium aurothiomalate) and in 23 samples of synovial tissue removed surgically at intervals during chrysotherapy in 5 patients. After the application of a highly specific staining technique, the cellular localization of gold was examined microscopically in various tissues: the amounts of gold in selected areas of the same specimens which had been examined microscopically were then measured by neutron activation analysis. During active chrysotherapy gold was abundant in synovial lining cells except where a fibrin layer was present on the surface; after stopping chrysotherapy, gold disappeared from the synovial lining cells. Gold accumulated progressively in the subsynovial connective tissues during chrysotherapy, but was not uniformly distributed, and bore no relationship to fluctuations in serum gold levels. Gold deposition was not confined to joint tissue, but was found within the macrophages of many organs, renal tubular epithelium, and, after recent chrysotherapy, in seminiferous tubules, hepatocytes, and adrenal cortical cells. Gold persisted in synovial and other tissues for up to 23 years after chrysotherapy was stopped. The overall findings indicated that gold is selectively concentrated within inflamed synovial tissues during chrysotherapy."}
{"id": "PMID:1087552", "title": "Histocompatibility antigens in psoriatic arthritis.", "content": "Histocompatibility (HL-A) antigen typing was performed on 82 patients with psoriatic arthritis. The prevalance of HL-AB27 was increased (28%) compared with that in a control group (7-5%), and this was most marked in those with spinal changes. The prevalence in patients with sacroiliitis and syndesmophytes was 75%, with sacroiliitis alone 78%, but with syndesmophytes and normal sacroiliac joints only 36%. The overall prevalence in psoriatic spondylitis was 60%, with a prevalence of 71% in those fulfilling the New York criteria for anklosing spondylitis. The prevalence was not increased in those with only peripheral arthritis (11%). The prevalence of HLA-B13 was decreased in those with psoriatic spondylitis, though this was not statistically significant. The overall prevalence of HLA-BW17 was increased, this being particularly so in those with syndesmophytes, but values were not significant.", "contents": "Histocompatibility antigens in psoriatic arthritis. Histocompatibility (HL-A) antigen typing was performed on 82 patients with psoriatic arthritis. The prevalance of HL-AB27 was increased (28%) compared with that in a control group (7-5%), and this was most marked in those with spinal changes. The prevalence in patients with sacroiliitis and syndesmophytes was 75%, with sacroiliitis alone 78%, but with syndesmophytes and normal sacroiliac joints only 36%. The overall prevalence in psoriatic spondylitis was 60%, with a prevalence of 71% in those fulfilling the New York criteria for anklosing spondylitis. The prevalence was not increased in those with only peripheral arthritis (11%). The prevalence of HLA-B13 was decreased in those with psoriatic spondylitis, though this was not statistically significant. The overall prevalence of HLA-BW17 was increased, this being particularly so in those with syndesmophytes, but values were not significant."}
{"id": "PMID:1087554", "title": "Bleeding mucosal lesions in the upper gastrointestinal tracts of patients with oesophageal varices and hepatic cirrhosis.", "content": "Thirty panendoscopic examinations were carried out on 24 patients with oesophageal varices and hepatic cirrhosis who presented with gastrointestinal hemorrhage. A mucosal lesion, focal or diffuse, was found in the upper gastrointestinal tract in all but three instances. The findings confirmed previous reports that hemorrhage in patients with oesophageal varices frequently occurs from a source other than variceal. This study has also demonstrated that where hemorrhage occurs from oesophageal varices, erosions of the oesophageal mucosa may play a major role in its pathogenesis.", "contents": "Bleeding mucosal lesions in the upper gastrointestinal tracts of patients with oesophageal varices and hepatic cirrhosis. Thirty panendoscopic examinations were carried out on 24 patients with oesophageal varices and hepatic cirrhosis who presented with gastrointestinal hemorrhage. A mucosal lesion, focal or diffuse, was found in the upper gastrointestinal tract in all but three instances. The findings confirmed previous reports that hemorrhage in patients with oesophageal varices frequently occurs from a source other than variceal. This study has also demonstrated that where hemorrhage occurs from oesophageal varices, erosions of the oesophageal mucosa may play a major role in its pathogenesis."}
{"id": "PMID:1087556", "title": "[Hairy-leukocyte leukemia (tricholeukocyte). Report of a case].", "content": "A case of a woman suffering of hairy cells leukemia (tricholeucocytes) is described. The diagnosis of this leukemia was set two years after a splemectomy, due to the enlargement of the spleen. The patient was treated for a period of two years as a refractory anaemia and leucopenia (neutropenia). After admission the diagnosis was confirmed by the examination of periferal blood, cytologic and histologic examination of the bone marrow and after a subsequent examination of the spleen tissue. All these examinations showed the presence of typical hairy cells--tricholeucocytes in the periferal blood, bone marrow and spleen. On account of their own cytochemical examinations, and especialy immunological examinations by other authors, the authors of this presentation suppose that the tricholeucocytes most probably belong to B lymphocytes and that, therefore, this leukemia should be placed in the group of malignant lymphocytoproliferative diseases.", "contents": "[Hairy-leukocyte leukemia (tricholeukocyte). Report of a case]. A case of a woman suffering of hairy cells leukemia (tricholeucocytes) is described. The diagnosis of this leukemia was set two years after a splemectomy, due to the enlargement of the spleen. The patient was treated for a period of two years as a refractory anaemia and leucopenia (neutropenia). After admission the diagnosis was confirmed by the examination of periferal blood, cytologic and histologic examination of the bone marrow and after a subsequent examination of the spleen tissue. All these examinations showed the presence of typical hairy cells--tricholeucocytes in the periferal blood, bone marrow and spleen. On account of their own cytochemical examinations, and especialy immunological examinations by other authors, the authors of this presentation suppose that the tricholeucocytes most probably belong to B lymphocytes and that, therefore, this leukemia should be placed in the group of malignant lymphocytoproliferative diseases."}
{"id": "PMID:1087557", "title": "Inactivation of human thrombin in the presence of human alpha1-proteinase inhibitor.", "content": "Both the clotting and esterase activities of thrombin are inhibited by alpha1-proteinase inhibitor (alpha1-antitrypsin). The inhibition is a time-and temperature-dependent reaction which is proportional to the molar ratio of thrombin to inhibitor. Both the active-site serine residue of thrombin and the reactive-site lysine residue of alpha1-proteinase inhibitor are involved. alpha1-Proteinase inhibitor forms a 1:1 complex with thrombin that is comparable with the complex formed with trypsin and other proteinases. Incubation of the inhibitor with excess of thrombin, however, results in inactivation of nearly all the enzyme, even though only as much complex is formed as alpha1-proteinase inhibitor present. A portion of the remaining thrombin apparently aggregates. These results suggest that the mechanism for inhibition of thrombin may not be exactly the same as for trypsin, which is inhibited only to the extent to which complex is formed.", "contents": "Inactivation of human thrombin in the presence of human alpha1-proteinase inhibitor. Both the clotting and esterase activities of thrombin are inhibited by alpha1-proteinase inhibitor (alpha1-antitrypsin). The inhibition is a time-and temperature-dependent reaction which is proportional to the molar ratio of thrombin to inhibitor. Both the active-site serine residue of thrombin and the reactive-site lysine residue of alpha1-proteinase inhibitor are involved. alpha1-Proteinase inhibitor forms a 1:1 complex with thrombin that is comparable with the complex formed with trypsin and other proteinases. Incubation of the inhibitor with excess of thrombin, however, results in inactivation of nearly all the enzyme, even though only as much complex is formed as alpha1-proteinase inhibitor present. A portion of the remaining thrombin apparently aggregates. These results suggest that the mechanism for inhibition of thrombin may not be exactly the same as for trypsin, which is inhibited only to the extent to which complex is formed."}
{"id": "PMID:1087558", "title": "Comparison of amphibian whole-cell and cell-free activity towards different transferrins.", "content": "1. A cell-free preparation from immature-erythrocytes of the bullfrog (Rana catesbeiana) was about equally active towards iron bound to homologous, rabbit or human transferrin. 2. In contrast with the cell-free system, whole immature erythrocytes exhibited optimum, intermediate or low uptake of iron from the above and from other transferrins.", "contents": "Comparison of amphibian whole-cell and cell-free activity towards different transferrins. 1. A cell-free preparation from immature-erythrocytes of the bullfrog (Rana catesbeiana) was about equally active towards iron bound to homologous, rabbit or human transferrin. 2. In contrast with the cell-free system, whole immature erythrocytes exhibited optimum, intermediate or low uptake of iron from the above and from other transferrins."}
{"id": "PMID:1087553", "title": "[Relations between serum apha-antitrypsin level and neoplasms].", "content": "Alpha1-antitrypsin levels were determined in patients with bronchopneumopathies and in others with various forms of neoplasia. No relationship between levels and disease was noted in the first group, whereas a marked increase in values was observed in the second, particularly in the case of subjects with lung cancer. The interestiang aspects of this finding are discussed.", "contents": "[Relations between serum apha-antitrypsin level and neoplasms]. Alpha1-antitrypsin levels were determined in patients with bronchopneumopathies and in others with various forms of neoplasia. No relationship between levels and disease was noted in the first group, whereas a marked increase in values was observed in the second, particularly in the case of subjects with lung cancer. The interestiang aspects of this finding are discussed."}
{"id": "PMID:1087561", "title": "[Immunosuppressive treatment of rheumatic diseases. Experimental bases of a rational concept of therapeutic approach (author's transl)].", "content": "For treatment of diseases such as rheumatoid arthritis or systemic lupus erythematodes, which are initiated or sustained by immune-pathological mechanisms, various \"immunosuppressive\" drugs are used. There are conflicting data as to the benefit of this type of therapy. In this paper it is attempted to define a base for a more differentiated application of available drugs, since the present therapeutic approach seems rather empiric or is deducted from analogy to selected animal experiments. The investigations presented focus primarily on the behaviour of the small and medium lymphocytes of the organism, the adopted carriers of immunological (as well as autoimmune) reactivity, under conventional conditions (and under the influence of suitable drugs) as a biological supposition for the activity of \"immunosuppressives\". In rabbits, and mice, number and rate of proliferation of lymphoid cells is determined in untreated controls and animals treated with 6-mercaptopurine (6-MP) and cyclophosphamide (Cy), two immunosuppressive agents representing different types of pharmacological action. The elucidation why in rabbits both substances are equally immunosuppressive, whereas in mice only Cy has significant immunosuppressive activity, yields the base for a therapeutic concept of clinical immunosuppression. This species dependent activity of 6-MP can be explained by different proliferation kinetics of lymphoid cells in mouse and rabbit. Lymphocytes of the rabbit, compared to those of mice, are short-lived and have a distinctly higher proliferation rate. Thus, 6-MP, as an antiproliferative agent, leads, in the rabbit (under long-term as well as single-dose therapy) to a significant reduction of the number of small lymphocytes, whereas it reduces the long-lived lymphocytes of the mouse only marginally, thus explaining the good immunosuppressive potency in the rabbit and failure in the mouse. Cy leads, in both species, to a marked reduction of small lymphocytes and affects the long-lived cells of the mouse as well, resulting in high immunosuppressive potency in both species. In the NZB mouse, a well-fitting model of human lupus erythomatodes, Cy is successful in prophylaxis and therapy. A similar therapeutic effect cannot be obtained with 6-MP. Neither of the two groups of substances revealed selective activity on circulating T- or B-cells. According to the literature available, lymphocytes in humans are predominantly long-lived, too. Accordingly, Cy possesses a good immunosuppressive potency in man, too. Its therapeutic success is paralleled by a reduction of small lymphocytes. In conclusion, a true basic immunosuppressive therapy of autoimmune diseases in humans will primarily be possible by aid of substances which act through a cytotoxic mechanism and are thus able to affect even the long-lived human lymphocytes. In contrast, a substance acting purely through interference with certain steps of cell proliferation will predominantly remain restricted to an antiphlogistic use.", "contents": "[Immunosuppressive treatment of rheumatic diseases. Experimental bases of a rational concept of therapeutic approach (author's transl)]. For treatment of diseases such as rheumatoid arthritis or systemic lupus erythematodes, which are initiated or sustained by immune-pathological mechanisms, various \"immunosuppressive\" drugs are used. There are conflicting data as to the benefit of this type of therapy. In this paper it is attempted to define a base for a more differentiated application of available drugs, since the present therapeutic approach seems rather empiric or is deducted from analogy to selected animal experiments. The investigations presented focus primarily on the behaviour of the small and medium lymphocytes of the organism, the adopted carriers of immunological (as well as autoimmune) reactivity, under conventional conditions (and under the influence of suitable drugs) as a biological supposition for the activity of \"immunosuppressives\". In rabbits, and mice, number and rate of proliferation of lymphoid cells is determined in untreated controls and animals treated with 6-mercaptopurine (6-MP) and cyclophosphamide (Cy), two immunosuppressive agents representing different types of pharmacological action. The elucidation why in rabbits both substances are equally immunosuppressive, whereas in mice only Cy has significant immunosuppressive activity, yields the base for a therapeutic concept of clinical immunosuppression. This species dependent activity of 6-MP can be explained by different proliferation kinetics of lymphoid cells in mouse and rabbit. Lymphocytes of the rabbit, compared to those of mice, are short-lived and have a distinctly higher proliferation rate. Thus, 6-MP, as an antiproliferative agent, leads, in the rabbit (under long-term as well as single-dose therapy) to a significant reduction of the number of small lymphocytes, whereas it reduces the long-lived lymphocytes of the mouse only marginally, thus explaining the good immunosuppressive potency in the rabbit and failure in the mouse. Cy leads, in both species, to a marked reduction of small lymphocytes and affects the long-lived cells of the mouse as well, resulting in high immunosuppressive potency in both species. In the NZB mouse, a well-fitting model of human lupus erythomatodes, Cy is successful in prophylaxis and therapy. A similar therapeutic effect cannot be obtained with 6-MP. Neither of the two groups of substances revealed selective activity on circulating T- or B-cells. According to the literature available, lymphocytes in humans are predominantly long-lived, too. Accordingly, Cy possesses a good immunosuppressive potency in man, too. Its therapeutic success is paralleled by a reduction of small lymphocytes. In conclusion, a true basic immunosuppressive therapy of autoimmune diseases in humans will primarily be possible by aid of substances which act through a cytotoxic mechanism and are thus able to affect even the long-lived human lymphocytes. In contrast, a substance acting purely through interference with certain steps of cell proliferation will predominantly remain restricted to an antiphlogistic use."}
{"id": "PMID:1087562", "title": "Pharmacological and toxicological studies with 1-benzyl-3-(2,3-dihydroxypropoxy)indazole.", "content": "1-Benzyl-3-(2,3-dihydroxypropoxy)-indazole (benzidol) is a novel indazolane derivative with marked antiinflammatory, analgesic and antipyretic properties. Its acute toxicity is three times less than that of benzidamine and five times less than that of phenylbutazone. Rats, mice, guinea pigs and rabbits treated with benzidol during 6 months at doses of 5, 50 and 200 mg/kg did not present any pathological alteration. The anti-inflammatory activity of benzidol is similar to that of phenylbutazone and higher than that of benzidamine. The analgesic and antipyretic properties are also considerable. Benzidol is better tolerated than the mentioned drugs with similar activities.", "contents": "Pharmacological and toxicological studies with 1-benzyl-3-(2,3-dihydroxypropoxy)indazole. 1-Benzyl-3-(2,3-dihydroxypropoxy)-indazole (benzidol) is a novel indazolane derivative with marked antiinflammatory, analgesic and antipyretic properties. Its acute toxicity is three times less than that of benzidamine and five times less than that of phenylbutazone. Rats, mice, guinea pigs and rabbits treated with benzidol during 6 months at doses of 5, 50 and 200 mg/kg did not present any pathological alteration. The anti-inflammatory activity of benzidol is similar to that of phenylbutazone and higher than that of benzidamine. The analgesic and antipyretic properties are also considerable. Benzidol is better tolerated than the mentioned drugs with similar activities."}
{"id": "PMID:1087564", "title": "Use of noninvasive methods in evaluation of left ventricular performance in coronary artery disease. II: Change in systolic time intervals after coronary artery bypass in patients with stable angina.", "content": "Serial recording of systolic time intervals (STI) was used to assess changes in left ventricular (LV) function in 92 patients with stable angina undergoing coronary angiography and LV cineangiography. On the basis of angiography, patients were placed in the following study groups: mild or absent coronary artery disease group -21 patients with less than 70 per cent occlusion of all coronary arteries, considered to have non-significant coronary artery disease and not needing bypass surgery; surgical bypass group-52 patients with evidence of significant (less than 70%) obstruction of at least one coronary artery who underwent myocardial revascularization; and medical group-19 patients with evidence of significant coronary artery disease not undergoing bypass surgery. Measured STI, corrected for heart rate and sex, were expressed as indices. No significant differences were noted in the mean STI from the time of initial evaluation to second evaluation 3 to 6 months later in either the mold or absent coronary artery disease patients or the medical group. In the surgical bypass group, however, serial studies at initial angiographic evaulation and 3 to 6 months after bypass surgery showed a shortened pre-ejection period (PEP) from 150 +/- 20 to 142 +/- 16 mn (P less than 0-001), prolonged left ventricular ejection time (LVET) from 396 +/- 21 to 409 +/- 14 ms (P less than 0-02), and decreased PEP/LVET from 0-45 +/- 0-11 to 0-04 +/- 0-07 (P less than 0-02). Paired data analysis of the three groups also showed a significant change only within the surgical bypass group from initial to find study, with a shortened PEP (P less than 0-002), prolonged LVET (P less than 0-001), and decreased PEP/LVET (P less than 0-001). These data suggest improvement in LV function in patients with significant coronary artery disease within a few months of myocardial revascularization, without demonstrable change in STI in those patients with coronary artery disease not subjected to revascularization.", "contents": "Use of noninvasive methods in evaluation of left ventricular performance in coronary artery disease. II: Change in systolic time intervals after coronary artery bypass in patients with stable angina. Serial recording of systolic time intervals (STI) was used to assess changes in left ventricular (LV) function in 92 patients with stable angina undergoing coronary angiography and LV cineangiography. On the basis of angiography, patients were placed in the following study groups: mild or absent coronary artery disease group -21 patients with less than 70 per cent occlusion of all coronary arteries, considered to have non-significant coronary artery disease and not needing bypass surgery; surgical bypass group-52 patients with evidence of significant (less than 70%) obstruction of at least one coronary artery who underwent myocardial revascularization; and medical group-19 patients with evidence of significant coronary artery disease not undergoing bypass surgery. Measured STI, corrected for heart rate and sex, were expressed as indices. No significant differences were noted in the mean STI from the time of initial evaluation to second evaluation 3 to 6 months later in either the mold or absent coronary artery disease patients or the medical group. In the surgical bypass group, however, serial studies at initial angiographic evaulation and 3 to 6 months after bypass surgery showed a shortened pre-ejection period (PEP) from 150 +/- 20 to 142 +/- 16 mn (P less than 0-001), prolonged left ventricular ejection time (LVET) from 396 +/- 21 to 409 +/- 14 ms (P less than 0-02), and decreased PEP/LVET from 0-45 +/- 0-11 to 0-04 +/- 0-07 (P less than 0-02). Paired data analysis of the three groups also showed a significant change only within the surgical bypass group from initial to find study, with a shortened PEP (P less than 0-002), prolonged LVET (P less than 0-001), and decreased PEP/LVET (P less than 0-001). These data suggest improvement in LV function in patients with significant coronary artery disease within a few months of myocardial revascularization, without demonstrable change in STI in those patients with coronary artery disease not subjected to revascularization."}
{"id": "PMID:1087566", "title": "Characterization of serum factors modulating splenic cytotoxicity in a syngeneic rat tumour system.", "content": "During the terminal stages of tumour growth (6-8 weeks) in Wistar rats bearing a syngeneic squamous cell carcinoma (Sp1), their sera can block in vitro anti-tumour cytotoxicity by immune splenic T lymphocytes. At an earlier stage of tumour growth (4-6 weeks) the sera do not block this cytotoxicity, but can induce anti-tumour cytotoxicity by non-immune spleen cells in the absence of complement. Sera taken at these 2 stages of tumour growth have been fractionated by ion-exchange chromatography, using DEAE-cellulose. The fractions have been examined by immunoelectrophoresis and tested for anti-tumour reactivity. Blocking activity was found in the Week-8 serum fraction eluted with 0-005M phosphate buffer, pH 7-4, whilst the \"cytotoxic\" activity of Week-4 serum was eluted with 0-02M phosphate buffer, pH 6-2. It is suggested that different IgG sub-classes are responsible for the 2 activities.", "contents": "Characterization of serum factors modulating splenic cytotoxicity in a syngeneic rat tumour system. During the terminal stages of tumour growth (6-8 weeks) in Wistar rats bearing a syngeneic squamous cell carcinoma (Sp1), their sera can block in vitro anti-tumour cytotoxicity by immune splenic T lymphocytes. At an earlier stage of tumour growth (4-6 weeks) the sera do not block this cytotoxicity, but can induce anti-tumour cytotoxicity by non-immune spleen cells in the absence of complement. Sera taken at these 2 stages of tumour growth have been fractionated by ion-exchange chromatography, using DEAE-cellulose. The fractions have been examined by immunoelectrophoresis and tested for anti-tumour reactivity. Blocking activity was found in the Week-8 serum fraction eluted with 0-005M phosphate buffer, pH 7-4, whilst the \"cytotoxic\" activity of Week-4 serum was eluted with 0-02M phosphate buffer, pH 6-2. It is suggested that different IgG sub-classes are responsible for the 2 activities."}
{"id": "PMID:1087568", "title": "Acquired von Willebrand syndrome with inhibitors both to factor VIII clotting activity and ristocetin-induced platelet aggregation.", "content": "A case of acquired von Willebrand's syndrome (vWs) is described which appeared to be due to antibodies directed against factor VIII clotting activity (FVIIIC), factor VIII-related antigen (FVIIIRAg) and von Willebrand factor. The antibodies directed against FVIIIRAg was demonstrated by the inhibitory effect of a platelet eluate on Ristocetin-induced aggregation of normal platelets. This effect was not shown by the patient's platelet-poor plasma alone, nor could it be demonstrated in platelet eluates from 13 other patients who had antibodies to FVIIIC but in whom there was no evidence of an acquired vWs.", "contents": "Acquired von Willebrand syndrome with inhibitors both to factor VIII clotting activity and ristocetin-induced platelet aggregation. A case of acquired von Willebrand's syndrome (vWs) is described which appeared to be due to antibodies directed against factor VIII clotting activity (FVIIIC), factor VIII-related antigen (FVIIIRAg) and von Willebrand factor. The antibodies directed against FVIIIRAg was demonstrated by the inhibitory effect of a platelet eluate on Ristocetin-induced aggregation of normal platelets. This effect was not shown by the patient's platelet-poor plasma alone, nor could it be demonstrated in platelet eluates from 13 other patients who had antibodies to FVIIIC but in whom there was no evidence of an acquired vWs."}
{"id": "PMID:1087563", "title": "[Aortic coarctation. Indications and results of surgical treatment. 16 years of experience].", "content": "Eighty-nine patients were treated surgically for coarctation of the aorta in a period of 16 years. In 79, the operative procedure consisted of excision of the coarcted segment and primary anastomosis; in 7 cases of hypoplastic arch and in 3 of aneurysm of the aorta, some form of aortoplasty utilizing the isthmus and the subclavian artery, or the use of a dacron prosthesis were required. In a group of 33 patients under 2 years, 76% had associated cardiac anomalies and 100% heart failure, which made immediate surgical treatment mandatory with a mortality of 42% and a recoarctation rate of 10.5%. Out of 56 cases over 2 years, 46% had other cardiac malformations; however, a less severe clinical conditions allowed for an elective operation, with a mortality of 1.8% and a recoarctation rate of 1.8%. This experience and the review of the literature support the concept of elective surgical treatment for coarctation of the aorta around the age of 4 years, prior to the occurrence of hypertension or its complications. In the neonate and infant, surgery is justified in the presence of heart failure non-responsive to medical treatment within 24 to 48 hours. A deeper understanding of the pathophysiology of the malformation in the very young and a better perioperative management of neonates, have led to a decline in mortality in recent years.", "contents": "[Aortic coarctation. Indications and results of surgical treatment. 16 years of experience]. Eighty-nine patients were treated surgically for coarctation of the aorta in a period of 16 years. In 79, the operative procedure consisted of excision of the coarcted segment and primary anastomosis; in 7 cases of hypoplastic arch and in 3 of aneurysm of the aorta, some form of aortoplasty utilizing the isthmus and the subclavian artery, or the use of a dacron prosthesis were required. In a group of 33 patients under 2 years, 76% had associated cardiac anomalies and 100% heart failure, which made immediate surgical treatment mandatory with a mortality of 42% and a recoarctation rate of 10.5%. Out of 56 cases over 2 years, 46% had other cardiac malformations; however, a less severe clinical conditions allowed for an elective operation, with a mortality of 1.8% and a recoarctation rate of 1.8%. This experience and the review of the literature support the concept of elective surgical treatment for coarctation of the aorta around the age of 4 years, prior to the occurrence of hypertension or its complications. In the neonate and infant, surgery is justified in the presence of heart failure non-responsive to medical treatment within 24 to 48 hours. A deeper understanding of the pathophysiology of the malformation in the very young and a better perioperative management of neonates, have led to a decline in mortality in recent years."}
{"id": "PMID:1087569", "title": "Practolol and ocular toxicity. Antibodies in serum and tears.", "content": "Serological studies in 22 patients presenting with ocular disease attributable to dosage with the beta-blocking agent practolol revealed a raised incidence of antinuclear antibodies. There was also a marginal increase in the incidence of antibodies to smooth muscle in the more severely affected individuals but the incidence of ther autoantibodies and levels of IgG, IgA, and IgM were within normal limits. Semi-quantitative analysis of tears from 14 of the patients showed absence or near absence in the more severely affected patients of secretory IgA, which is indicative of damage to the lacrimal gland. Other immunological parameters in the tears were normal.", "contents": "Practolol and ocular toxicity. Antibodies in serum and tears. Serological studies in 22 patients presenting with ocular disease attributable to dosage with the beta-blocking agent practolol revealed a raised incidence of antinuclear antibodies. There was also a marginal increase in the incidence of antibodies to smooth muscle in the more severely affected individuals but the incidence of ther autoantibodies and levels of IgG, IgA, and IgM were within normal limits. Semi-quantitative analysis of tears from 14 of the patients showed absence or near absence in the more severely affected patients of secretory IgA, which is indicative of damage to the lacrimal gland. Other immunological parameters in the tears were normal."}
{"id": "PMID:1087570", "title": "Intracameral injection of gentamicin. Report of a case.", "content": "A case of bacterial endophthalmitis secondary to a penetrating injury was successfully treated with intracameral topical and subconjunctival injections of gentamicin. After treatment the patient had normal vision and there was no evidence of toxic side-effects on the anterior segment of the eye including the endothelium, angle structures, and lens. The potential pathogenicity of Staphylococcus epidermidis is again emphasized.", "contents": "Intracameral injection of gentamicin. Report of a case. A case of bacterial endophthalmitis secondary to a penetrating injury was successfully treated with intracameral topical and subconjunctival injections of gentamicin. After treatment the patient had normal vision and there was no evidence of toxic side-effects on the anterior segment of the eye including the endothelium, angle structures, and lens. The potential pathogenicity of Staphylococcus epidermidis is again emphasized."}
{"id": "PMID:1087571", "title": "Purification and properties of rabbit trypsin.", "content": "The purification of rabbit pancreatic trypsin (EC 3.4.21.4) by affinity chromatography on Trasylol-Sepharose is presented along with its physical, chemical and immunological relationship to other trypsins. The molecule is a single polypeptide chain, which immunologically cross-reacts with porcine trypsin, but not with rabbit acrosomal proteinase. Sequence homology with other mammalian trypsins is seen at the amino terminus.", "contents": "Purification and properties of rabbit trypsin. The purification of rabbit pancreatic trypsin (EC 3.4.21.4) by affinity chromatography on Trasylol-Sepharose is presented along with its physical, chemical and immunological relationship to other trypsins. The molecule is a single polypeptide chain, which immunologically cross-reacts with porcine trypsin, but not with rabbit acrosomal proteinase. Sequence homology with other mammalian trypsins is seen at the amino terminus."}
{"id": "PMID:1087572", "title": "[Mechanism of excitation and contraction uncoupling in frog and guinea pig myocardial fibers during block of slow sodium-calcium channels by compound D-600].", "content": "In the experiments performed on the strips of frog's atria and ventricle it was found that rhythmic stimulation facilitated the dissociation of excitation--contraction coupling caused by D-600 compound through the block of calcium channels, i.e. D-600 prevented the increase of tension in the series of contractions or even converted positive staircase into negative one. The first contraction was affected by D-600 to a lesser degree than the subsequent ones. Guinea pig's left auriculus depressing action of D-600 was found to be strongly dependent on the frequency of stimulation. The data obtained were analysed by the model of excitation--contraction coupling. It was concluded that in amphibians as well as in mammalians tension amplitude during rhythmic activity of myocardial cells is determined mainly by Ca inflow through the excitable calcium channels. On the other hand the amplitude of the first contraction depends on the storage content determined by Ca inflow through the Ca channels open at rest. These latter channels have low sensitivity to D-600 and are better presented in atrium than in ventriculum. An increase of contraction tension observed in low sodium medium took place also under the action of D-600; in this case Ca storage was replenished by the Na-Ca exchange diffusion.", "contents": "[Mechanism of excitation and contraction uncoupling in frog and guinea pig myocardial fibers during block of slow sodium-calcium channels by compound D-600]. In the experiments performed on the strips of frog's atria and ventricle it was found that rhythmic stimulation facilitated the dissociation of excitation--contraction coupling caused by D-600 compound through the block of calcium channels, i.e. D-600 prevented the increase of tension in the series of contractions or even converted positive staircase into negative one. The first contraction was affected by D-600 to a lesser degree than the subsequent ones. Guinea pig's left auriculus depressing action of D-600 was found to be strongly dependent on the frequency of stimulation. The data obtained were analysed by the model of excitation--contraction coupling. It was concluded that in amphibians as well as in mammalians tension amplitude during rhythmic activity of myocardial cells is determined mainly by Ca inflow through the excitable calcium channels. On the other hand the amplitude of the first contraction depends on the storage content determined by Ca inflow through the Ca channels open at rest. These latter channels have low sensitivity to D-600 and are better presented in atrium than in ventriculum. An increase of contraction tension observed in low sodium medium took place also under the action of D-600; in this case Ca storage was replenished by the Na-Ca exchange diffusion."}
{"id": "PMID:1087575", "title": "Emergency abdominal surgery in the aged.", "content": "The results of 375 emergency abdominal operations in elderly patients over the age of 75 years are presented and discussed in detail. There has been a fourfold increase in the population over the age of 75 years in England and Wales during the 60-year period from 1911 to 1971. The overall mortality of these patients of 31-7 per cent is unrelated to the age of the patient, but appears to be directly related to the severity of the surgical condition and to cardiorespiratory complications. Our approach to these problems is presented and discussed.", "contents": "Emergency abdominal surgery in the aged. The results of 375 emergency abdominal operations in elderly patients over the age of 75 years are presented and discussed in detail. There has been a fourfold increase in the population over the age of 75 years in England and Wales during the 60-year period from 1911 to 1971. The overall mortality of these patients of 31-7 per cent is unrelated to the age of the patient, but appears to be directly related to the severity of the surgical condition and to cardiorespiratory complications. Our approach to these problems is presented and discussed."}
{"id": "PMID:1087577", "title": "Sensitivity of Trichomonas vaginalis to metronidazole, tinidazole, and nifuratel in vitro.", "content": "Prompted by the sensitivity of trichomonads to metronidazole and nifuratel in clinical practice, a study was conducted in 1971-1972 of 63 consecutive strains of Trichomonas vaginalis isolated from women with clinically refractory vaginal discharge. Their susceptibility to metronidazole, tinidazole, and nifuratel was tested, using a serial tube dilution technique. The minimum concentrations which in 48 hrs caused immobilization and lysis of trichomonads cultured in Diamond's medium was assessed. No differences in drug potency could be determined. The median trichomonistatic and trichomonicidal concentrations were 0-1 and 0-6 mug/ml. respectively when using an inoculum of 10,000 organisms per ml. An inoculum of 100,000 per ml. resulted in inhibitory concentrations of 1-0 and killing concentrations of 3-3 mug./ml. These levels are readily attained in blood and vaginal tissue after oral ingestion of the two imidazole derivatives. Thus, metronidazole has maintained its efficacy since it was first introduced more than a decade ago. The few therapeutic failures with metronidazole and tinidazole are considered to have been caused by pharmacokinetic deficiencies in the patients, or by re-infection.", "contents": "Sensitivity of Trichomonas vaginalis to metronidazole, tinidazole, and nifuratel in vitro. Prompted by the sensitivity of trichomonads to metronidazole and nifuratel in clinical practice, a study was conducted in 1971-1972 of 63 consecutive strains of Trichomonas vaginalis isolated from women with clinically refractory vaginal discharge. Their susceptibility to metronidazole, tinidazole, and nifuratel was tested, using a serial tube dilution technique. The minimum concentrations which in 48 hrs caused immobilization and lysis of trichomonads cultured in Diamond's medium was assessed. No differences in drug potency could be determined. The median trichomonistatic and trichomonicidal concentrations were 0-1 and 0-6 mug/ml. respectively when using an inoculum of 10,000 organisms per ml. An inoculum of 100,000 per ml. resulted in inhibitory concentrations of 1-0 and killing concentrations of 3-3 mug./ml. These levels are readily attained in blood and vaginal tissue after oral ingestion of the two imidazole derivatives. Thus, metronidazole has maintained its efficacy since it was first introduced more than a decade ago. The few therapeutic failures with metronidazole and tinidazole are considered to have been caused by pharmacokinetic deficiencies in the patients, or by re-infection."}
{"id": "PMID:1087578", "title": "Bimodal action of glycine on frog spinal motoneurones.", "content": "In the presence of procaine the changes in electrical potential caused by glycine in the ventral root of the isolated hemisected spinal cord of the frog have been shown to be compounded of both hyperpolarizing and depolarizing responses to the amino acid. From a comparison of the effects of changes in the perfusion medium on the potentials produced by glycine, beta-alanine and L-glutamate in the presence and absence of strychnine, it was concluded that glycine acts on a similar receptor to beta-alanine and causes a hyperpolarizing response which is blocked by strychnine. However, glycine has an additional, depolarizing, action which is usually the major effect and masks the hyperpolarizing response. The depolarizations produced by L-glutamate and glycine could be differentiated by their different ionic dependencies. The glycine depolarization was selectively decreased by a lowered sodium ion concentration in the medium while L-glutamate depolarizations were selectively enhanced by lowered potassium ion concentration.", "contents": "Bimodal action of glycine on frog spinal motoneurones. In the presence of procaine the changes in electrical potential caused by glycine in the ventral root of the isolated hemisected spinal cord of the frog have been shown to be compounded of both hyperpolarizing and depolarizing responses to the amino acid. From a comparison of the effects of changes in the perfusion medium on the potentials produced by glycine, beta-alanine and L-glutamate in the presence and absence of strychnine, it was concluded that glycine acts on a similar receptor to beta-alanine and causes a hyperpolarizing response which is blocked by strychnine. However, glycine has an additional, depolarizing, action which is usually the major effect and masks the hyperpolarizing response. The depolarizations produced by L-glutamate and glycine could be differentiated by their different ionic dependencies. The glycine depolarization was selectively decreased by a lowered sodium ion concentration in the medium while L-glutamate depolarizations were selectively enhanced by lowered potassium ion concentration."}
{"id": "PMID:1087580", "title": "Heart disease indicators in patients with aortocoronary bypass operation.", "content": "The incidence of cardiovascular disease risk factors and other indicators of cardiac impairment were studied in 478 patients prior to their aortocoronary vein bypass operation and 194 patients who had angiographic examination but did not undergo the operation because the clinical and coronary angiographic findings were not considered serious enough to warrant the bypass procedure. The patients referred for surgery had higher plasma cholesterol and triglyceride levels (259 and 219 vs 233 and 180 mg/100 ml), tended to be older (53 vs 49 years) and had more extensive occlusive disease (occlusion score 200 vs 70) than the group without the operation. There was little difference in the proportion of smokers (81 vs 83%) or prevalence of hypertension (33 vs 30%). In comparison with 9,964 participants of a local health screening program, both patient groups had markedly higher prevalence of smoking, hypertension, chest pain and previous myocardial infarcts.", "contents": "Heart disease indicators in patients with aortocoronary bypass operation. The incidence of cardiovascular disease risk factors and other indicators of cardiac impairment were studied in 478 patients prior to their aortocoronary vein bypass operation and 194 patients who had angiographic examination but did not undergo the operation because the clinical and coronary angiographic findings were not considered serious enough to warrant the bypass procedure. The patients referred for surgery had higher plasma cholesterol and triglyceride levels (259 and 219 vs 233 and 180 mg/100 ml), tended to be older (53 vs 49 years) and had more extensive occlusive disease (occlusion score 200 vs 70) than the group without the operation. There was little difference in the proportion of smokers (81 vs 83%) or prevalence of hypertension (33 vs 30%). In comparison with 9,964 participants of a local health screening program, both patient groups had markedly higher prevalence of smoking, hypertension, chest pain and previous myocardial infarcts."}
{"id": "PMID:1087582", "title": "[Kinetics and morphology of immune reactve cells in the spleen as observed uwing the immune adherence method. III. Spontaneous immunocytoadherence as influenced by total body irradiation].", "content": "The course of spontaneous immunocytoadherence as influenced by a total body 850 r irradiation was studied in the mouse spleen. Prior to the total body irradiation 0.87 RFC/100 cells occurring spontaneously, without any previous antigenic stimulus, were dislosed in the spleen. Twenty four hours after iraadiation 0.075 RFC/1000 cells were detected. This number dropped to 0.023 RFC/1000 cells at day 4 after irradiation. A qualitative morphological analysis showed the following values: 65,1% of the spontaneous RFC were small lymphocytes, 24.5% were medium-sized lymphocytes and 3% large lymphocytes; 1.1% were plasma cells and 5.4% macrophages. Twenty four hours after irradiation with a dose of 850 r the RFC contained 9.3% plasma cells, and 90.7% macrophages, the respective numbers on day 2 being 7.5% and 98.3%. At day 4, permitting to demonstrate the RFC in significant numbers there occurred macrophages only. The model experiment has shown that the formation of spontaneous rosettes represents a cirtually normal immune reaction.", "contents": "[Kinetics and morphology of immune reactve cells in the spleen as observed uwing the immune adherence method. III. Spontaneous immunocytoadherence as influenced by total body irradiation]. The course of spontaneous immunocytoadherence as influenced by a total body 850 r irradiation was studied in the mouse spleen. Prior to the total body irradiation 0.87 RFC/100 cells occurring spontaneously, without any previous antigenic stimulus, were dislosed in the spleen. Twenty four hours after iraadiation 0.075 RFC/1000 cells were detected. This number dropped to 0.023 RFC/1000 cells at day 4 after irradiation. A qualitative morphological analysis showed the following values: 65,1% of the spontaneous RFC were small lymphocytes, 24.5% were medium-sized lymphocytes and 3% large lymphocytes; 1.1% were plasma cells and 5.4% macrophages. Twenty four hours after irradiation with a dose of 850 r the RFC contained 9.3% plasma cells, and 90.7% macrophages, the respective numbers on day 2 being 7.5% and 98.3%. At day 4, permitting to demonstrate the RFC in significant numbers there occurred macrophages only. The model experiment has shown that the formation of spontaneous rosettes represents a cirtually normal immune reaction."}
{"id": "PMID:1087583", "title": "Definitions and terminology in cancer (tumour) etiology. An analysis aiming at proposals for a current internationally standardized terminology.", "content": "In an attempt to overcome the confusion that exists in the terminology of cancer (tumour) etiology, the author has analysed the current situation and proposed terms which, he hopes, will stimulate international discussion and lead eventually to an agreed standard terminology.", "contents": "Definitions and terminology in cancer (tumour) etiology. An analysis aiming at proposals for a current internationally standardized terminology. In an attempt to overcome the confusion that exists in the terminology of cancer (tumour) etiology, the author has analysed the current situation and proposed terms which, he hopes, will stimulate international discussion and lead eventually to an agreed standard terminology."}
{"id": "PMID:1087584", "title": "Poisoning caused by the consumption of organomercury-dressed seed in Iraq.", "content": "In 1971-72, a large outbreak of poisoning caused by the consumption of seed dressed with organomercury compound occurred in Iraq. International assistance was requested by the Government of Iraq in investigating this outbreak. The results of the investigations to date were presented at the Conference on Intoxication due to Alkylmercury Treated Seed, held in Baghdad, Iraq on 9-13 November 1974. This paper summarizes the main findings and recommendations of the Conference.", "contents": "Poisoning caused by the consumption of organomercury-dressed seed in Iraq. In 1971-72, a large outbreak of poisoning caused by the consumption of seed dressed with organomercury compound occurred in Iraq. International assistance was requested by the Government of Iraq in investigating this outbreak. The results of the investigations to date were presented at the Conference on Intoxication due to Alkylmercury Treated Seed, held in Baghdad, Iraq on 9-13 November 1974. This paper summarizes the main findings and recommendations of the Conference."}
{"id": "PMID:1087585", "title": "Feasibility of undertaking cancer incidence studies in rural areas of India.", "content": "In the rural areas of developing countries, modern medical facilities are well below optimum levels and death registration is not mandatory. In India, as a result of such a situation, very few studies have been undertaken on the incidence of cancer in the rural population, though 80% of the people live in villages. The paper presents cancer incidence rates observed in a rural area of India by means of a method involving the use of paramedical personnel for initial screening, to minimize the cost. A statistical evaluation of the results shows that the method can be used for registering common sites of cancer in an area where conventional cancer registration methods are not applicable.", "contents": "Feasibility of undertaking cancer incidence studies in rural areas of India. In the rural areas of developing countries, modern medical facilities are well below optimum levels and death registration is not mandatory. In India, as a result of such a situation, very few studies have been undertaken on the incidence of cancer in the rural population, though 80% of the people live in villages. The paper presents cancer incidence rates observed in a rural area of India by means of a method involving the use of paramedical personnel for initial screening, to minimize the cost. A statistical evaluation of the results shows that the method can be used for registering common sites of cancer in an area where conventional cancer registration methods are not applicable."}
{"id": "PMID:1087586", "title": "Intra-ocular pressure in onchocerciasis. Some preliminary results of a field evaluation.", "content": "Preliminary data are presented on the relationship between intra-ocular pressure and onchocerciasis. There was no significant difference in the mean ocular pressure between persons with no ocular onchocerciasis and those with microfilariae in the eye, but a significantly lower mean tension was observed in those with severe ocular onchocerciasis.", "contents": "Intra-ocular pressure in onchocerciasis. Some preliminary results of a field evaluation. Preliminary data are presented on the relationship between intra-ocular pressure and onchocerciasis. There was no significant difference in the mean ocular pressure between persons with no ocular onchocerciasis and those with microfilariae in the eye, but a significantly lower mean tension was observed in those with severe ocular onchocerciasis."}
{"id": "PMID:1087587", "title": "Immunization in India with trivalent and monovalent oral poliovirus vaccines of enhanced potency.", "content": "In an attempt to improve the serological response of infants in warm climates to oral poliovirus vaccine (OPV), the authors administered to 79 children between 6 and 41 weeks of age trivalent and monovalent OPV containing a virus dose 10 times as high as that found in the standard vaccine. The seroconversion rates following one dose of this trivalent OPV were 42% to type 1 poliovirus, 85% to type 2, and 31% to type 3. These rates are only slightly better than those previously reported after one dose of standard trivalent OPV and much lower than those achieved after 3 doses. The seroconversion rates following one dose of the monovalent OPV of enhanced potency were 89%, 93%, and 76%, respectively. These rates are comparable to those achieved after 5 doses of the standard trivalent OPV. Thus the refractoriness of host response was only partly overcome by enhancing the virus inoculum 10-fold.", "contents": "Immunization in India with trivalent and monovalent oral poliovirus vaccines of enhanced potency. In an attempt to improve the serological response of infants in warm climates to oral poliovirus vaccine (OPV), the authors administered to 79 children between 6 and 41 weeks of age trivalent and monovalent OPV containing a virus dose 10 times as high as that found in the standard vaccine. The seroconversion rates following one dose of this trivalent OPV were 42% to type 1 poliovirus, 85% to type 2, and 31% to type 3. These rates are only slightly better than those previously reported after one dose of standard trivalent OPV and much lower than those achieved after 3 doses. The seroconversion rates following one dose of the monovalent OPV of enhanced potency were 89%, 93%, and 76%, respectively. These rates are comparable to those achieved after 5 doses of the standard trivalent OPV. Thus the refractoriness of host response was only partly overcome by enhancing the virus inoculum 10-fold."}
{"id": "PMID:1087589", "title": "Yersinia enterocolitica : a review of its role in food hygiene.", "content": "Since Yersinia enterocolitica, now classified as a member of the Enterobacteriaceae, was recognized as a distinct species in 1964 it has been isolated with increasing frequency from man and animals (including dogs and pigs) and from some human foods. Y. enterocolitica infections are now seen as a cause for some concern in both human and veterinary medicine. The organism is commonly found in specimens from swine slaughterhouses and has been isolated from samples of market meat, vacuum-packed beef, mussels, oysters, and ice-cream. It has also been found in nonchlorinated well water used for drinking purposes. Infections in man therefore probably have an alimentary origin. Only 23 human infections were recorded in 1966 but the number increased to over 4000 in 1974. However, reported incidence is affected by growing awareness about the role of the organism in human and animal disease and by intensive laboratory analyses. While knowledge about the geographical distribution of Y. enterocolitica is still fragmentary it is clear that infections are very frequent in some parts of the world and probably common but unrecognized in many countries. The most common symptoms of Y. enterocolitica infections in man are fever, abdominal pain, and diarrhoea. In the USA most isolations in human infections were made from blood and mesenteric lymph node samples. The pathogenic mechanism is not known. In one experiment involving a human volunteer subject a dose of 3.5 x 10(9) organisms was required to produce an infection. Only recently has some success been obtained in establishing experimental infections in mice, guinea-pigs, rats, and rabbits. Laboratory cultivation techniques for Y. enterocolitica are described together with a table of minimal tests for characterizing the organism and two biotyping schema. Little is known about methods for controlling this disease, but environmental hygiene and sanitation with regard to food and water should apply.", "contents": "Yersinia enterocolitica : a review of its role in food hygiene. Since Yersinia enterocolitica, now classified as a member of the Enterobacteriaceae, was recognized as a distinct species in 1964 it has been isolated with increasing frequency from man and animals (including dogs and pigs) and from some human foods. Y. enterocolitica infections are now seen as a cause for some concern in both human and veterinary medicine. The organism is commonly found in specimens from swine slaughterhouses and has been isolated from samples of market meat, vacuum-packed beef, mussels, oysters, and ice-cream. It has also been found in nonchlorinated well water used for drinking purposes. Infections in man therefore probably have an alimentary origin. Only 23 human infections were recorded in 1966 but the number increased to over 4000 in 1974. However, reported incidence is affected by growing awareness about the role of the organism in human and animal disease and by intensive laboratory analyses. While knowledge about the geographical distribution of Y. enterocolitica is still fragmentary it is clear that infections are very frequent in some parts of the world and probably common but unrecognized in many countries. The most common symptoms of Y. enterocolitica infections in man are fever, abdominal pain, and diarrhoea. In the USA most isolations in human infections were made from blood and mesenteric lymph node samples. The pathogenic mechanism is not known. In one experiment involving a human volunteer subject a dose of 3.5 x 10(9) organisms was required to produce an infection. Only recently has some success been obtained in establishing experimental infections in mice, guinea-pigs, rats, and rabbits. Laboratory cultivation techniques for Y. enterocolitica are described together with a table of minimal tests for characterizing the organism and two biotyping schema. Little is known about methods for controlling this disease, but environmental hygiene and sanitation with regard to food and water should apply."}
{"id": "PMID:1087590", "title": "An international haematological survey.", "content": "Haematological surveys of adult population samples were conducted simultaneously in 12 countries, all but one of which are in Europe. Haematological estimations on samples from nine of the countries were made in one central laboratory. Differences between countries in the mean levels of haemoglobin (and haematocrit and red cell count) were found to be relatively small, and the prevalence of levels below the arbitrary WHO levels for anaemia were, on the whole, low. In males, the evidence suggests a fall in haemoglobin level throughout adult life, which increases slightly in advanced age. In females, there is no evidence of any important relationship between age and haemoglobin level.", "contents": "An international haematological survey. Haematological surveys of adult population samples were conducted simultaneously in 12 countries, all but one of which are in Europe. Haematological estimations on samples from nine of the countries were made in one central laboratory. Differences between countries in the mean levels of haemoglobin (and haematocrit and red cell count) were found to be relatively small, and the prevalence of levels below the arbitrary WHO levels for anaemia were, on the whole, low. In males, the evidence suggests a fall in haemoglobin level throughout adult life, which increases slightly in advanced age. In females, there is no evidence of any important relationship between age and haemoglobin level."}
{"id": "PMID:1087591", "title": "The practice of endocrinology in a rural setting: suggested guidelines.", "content": "For the purposes of medical practice, a ;rural setting' implies an environment with little or no facilities for the sophisticated laboratory investigations that may be needed for the diagnosis of certain endocrine disorders. Fortunately, however, most of the commoner endocrine disorders may be diagnosed from the patient's history and a physical examination, together if necessary with the observed response to therapy. A lack of facilities, therefore, need not hinder the effective practice of endocrinology in rural areas.", "contents": "The practice of endocrinology in a rural setting: suggested guidelines. For the purposes of medical practice, a ;rural setting' implies an environment with little or no facilities for the sophisticated laboratory investigations that may be needed for the diagnosis of certain endocrine disorders. Fortunately, however, most of the commoner endocrine disorders may be diagnosed from the patient's history and a physical examination, together if necessary with the observed response to therapy. A lack of facilities, therefore, need not hinder the effective practice of endocrinology in rural areas."}
{"id": "PMID:1087592", "title": "Plasma factors in delayed-type hypersensitivity. Augmentation of lymphocyte responses in borderline leprosy reactions.", "content": "The phytohaemagglutinin-induced responses of lymphocytes were found to be inhibited by plasma from patients with leprosy when compared with their responses in pooled serum from healthy donors. When patients developed reversal reactions, the initial inhibitory effect of their plasma was replaced by an augmentary effect on the responses to phytohaemagglutinin. The period of augmentation coincided with that of the reversal reaction in patients with borderline tuberculoid leprosy, but was delayed in patients with borderline lepromatous leprosy. The plasma from each leprosy patient was also observed to have the same effect on lymphocytes from unrelated individuals, showing that the inhibition and augmentation were due to factors in the plasma and not to a change in lymphocyte receptors. It is possible that the normal stable state of leprosy results from the presence of factors in plasma which act as a control mechanism, and that delayed hypersensitivity reactions may be caused by a breakdown of this control.", "contents": "Plasma factors in delayed-type hypersensitivity. Augmentation of lymphocyte responses in borderline leprosy reactions. The phytohaemagglutinin-induced responses of lymphocytes were found to be inhibited by plasma from patients with leprosy when compared with their responses in pooled serum from healthy donors. When patients developed reversal reactions, the initial inhibitory effect of their plasma was replaced by an augmentary effect on the responses to phytohaemagglutinin. The period of augmentation coincided with that of the reversal reaction in patients with borderline tuberculoid leprosy, but was delayed in patients with borderline lepromatous leprosy. The plasma from each leprosy patient was also observed to have the same effect on lymphocytes from unrelated individuals, showing that the inhibition and augmentation were due to factors in the plasma and not to a change in lymphocyte receptors. It is possible that the normal stable state of leprosy results from the presence of factors in plasma which act as a control mechanism, and that delayed hypersensitivity reactions may be caused by a breakdown of this control."}
{"id": "PMID:1087593", "title": "Studies on the immune status of children with acute lymphocytic leukaemia. I. Early phase before and after first remission.", "content": "Seventeen children of 2-15 years of age with newly diagnosed untreated acute lymphatic leukaemia (ALL) were evaluated with a number of immunological tests: for humoral immunity serum immunoglobulins and reactive antibody formation against three antigens (diphtheria, tetanus, KLH); for cell-mediated immunity in vitro response of blood lymphocytes against PHA; membrane characteristics of blood lymphocytes and lymphoid blasts for both B and T cells. The tests were repeated in thirteen patients who attained full remission. In the majority (twelve cases) no surface markers were detected (null-cell leukaemia), one patient fulfilled the criteria for a T-cell leukaemia with thymoma. Four patients had rather high absolute B-cell counts, but did not fulfill all the criteria for B-cell leukaemia; three of them died before remission. Immune globulin concentrations were only slightly changed, antibody formation, both primary and anamnestic, was possible. PHA response was extremely low in the initial phase, but normal immediately after remission. During remission all patients were markedly depleted in both their B- and T-cell compartment.", "contents": "Studies on the immune status of children with acute lymphocytic leukaemia. I. Early phase before and after first remission. Seventeen children of 2-15 years of age with newly diagnosed untreated acute lymphatic leukaemia (ALL) were evaluated with a number of immunological tests: for humoral immunity serum immunoglobulins and reactive antibody formation against three antigens (diphtheria, tetanus, KLH); for cell-mediated immunity in vitro response of blood lymphocytes against PHA; membrane characteristics of blood lymphocytes and lymphoid blasts for both B and T cells. The tests were repeated in thirteen patients who attained full remission. In the majority (twelve cases) no surface markers were detected (null-cell leukaemia), one patient fulfilled the criteria for a T-cell leukaemia with thymoma. Four patients had rather high absolute B-cell counts, but did not fulfill all the criteria for B-cell leukaemia; three of them died before remission. Immune globulin concentrations were only slightly changed, antibody formation, both primary and anamnestic, was possible. PHA response was extremely low in the initial phase, but normal immediately after remission. During remission all patients were markedly depleted in both their B- and T-cell compartment."}
{"id": "PMID:1087594", "title": "Studies on the immune status of children with acute lymphocytic leukaemia. II. In remission with and without cytostatic treatment.", "content": "In eleven children with acute lymphocytic leukaemia (ALL), cytostatic treatment was stopped after sustained remission lasting for 9 months--6 1/2 years. Their immunological status was monitored every 6--12 weeks during the first year after cessation of therapy. Rebound of depressed parameters was observed for absolute lymphocyte, B-cell and T-cell counts and for immunoglobulins. A simple follow-up scheme for such patients is proposed.", "contents": "Studies on the immune status of children with acute lymphocytic leukaemia. II. In remission with and without cytostatic treatment. In eleven children with acute lymphocytic leukaemia (ALL), cytostatic treatment was stopped after sustained remission lasting for 9 months--6 1/2 years. Their immunological status was monitored every 6--12 weeks during the first year after cessation of therapy. Rebound of depressed parameters was observed for absolute lymphocyte, B-cell and T-cell counts and for immunoglobulins. A simple follow-up scheme for such patients is proposed."}
{"id": "PMID:1087595", "title": "Lymphocyte response to IgG in patients with ankylosing spondylitis and their families.", "content": "Lymphocyte responsiveness to IgG was measured by an agarose method in nine patients with ankylosing spondylitis (AS), one patient with Reiter's Syndrome (RS), and thirty-six of their family members. Similar studies were also performed in five patients with rheumatoid arthritis (RA) and twenty-nine of their first degree relatives as well as in seven control families (twenty-seven subjects). Lymphocytes from the ten spondylitic patients and twenty-four of thirty-six family members responded in vitro to autologous IgG. Although most of these subjects had the histocompatibility antigen, B27, there was no association between B27 and response to IgG. Four of the five patients with RA and twenty of their twenty-nine first degree relatives responded in vitro to IgG, whereas only six of twenty-seven control family members gave a positive reaction. There was no difference in the incidence of antiglobulins (detected by agglutination tests) in the family members of patients with AS and RA or in control family members. These data indicate that lymphocyte responsiveness to IgG is the only aberrant immune response thus far described which is shared by patients with AS and RA and their family members.", "contents": "Lymphocyte response to IgG in patients with ankylosing spondylitis and their families. Lymphocyte responsiveness to IgG was measured by an agarose method in nine patients with ankylosing spondylitis (AS), one patient with Reiter's Syndrome (RS), and thirty-six of their family members. Similar studies were also performed in five patients with rheumatoid arthritis (RA) and twenty-nine of their first degree relatives as well as in seven control families (twenty-seven subjects). Lymphocytes from the ten spondylitic patients and twenty-four of thirty-six family members responded in vitro to autologous IgG. Although most of these subjects had the histocompatibility antigen, B27, there was no association between B27 and response to IgG. Four of the five patients with RA and twenty of their twenty-nine first degree relatives responded in vitro to IgG, whereas only six of twenty-seven control family members gave a positive reaction. There was no difference in the incidence of antiglobulins (detected by agglutination tests) in the family members of patients with AS and RA or in control family members. These data indicate that lymphocyte responsiveness to IgG is the only aberrant immune response thus far described which is shared by patients with AS and RA and their family members."}
{"id": "PMID:1087596", "title": "Intramural hematoma of the esophagus: a complication of carbon tetrachloride intoxication with acute renal failure.", "content": "Following a period of prolonged severe vomiting, an intramural esophageal hematoma could be demonstrated by endoscopy and by X-ray in a 21 year old patient with hepatic and renal failure after exposure to carbon tetrachloride. The hematoma resolved spontaneously. The intramural hematoma is thought to have developed from a microdissection of the esophageal wall in the presence of a hemorrhagic diathesis.", "contents": "Intramural hematoma of the esophagus: a complication of carbon tetrachloride intoxication with acute renal failure. Following a period of prolonged severe vomiting, an intramural esophageal hematoma could be demonstrated by endoscopy and by X-ray in a 21 year old patient with hepatic and renal failure after exposure to carbon tetrachloride. The hematoma resolved spontaneously. The intramural hematoma is thought to have developed from a microdissection of the esophageal wall in the presence of a hemorrhagic diathesis."}
{"id": "PMID:1087604", "title": "Time course of hypothalamic CRF activity after the administration of two different stresses.", "content": "The time course of hypothalamic corticotropin-releasing factor (CRF) activity after the administration of ether stress was different from that after immobilization stress. The maximal response CRF activity was observed 2 min after ether stress, followed by a precipitous decrease 5 min after the stress. A gradual increase of CRF activity was subsequently observed for several minutes with fluctuated changes. Thus, response pattern was vibratory. But under immobilization stress, markedly fluctuant changes of CRF activity seen in the case of ether stress did not appear after the maximal response observed at 2 min, indicating that the response pattern was not vibratory. On the other hand, the concentration of plasma corticosterone increased significantly 5 min after the ether or immobilization stress with the peak value around 17 min.", "contents": "Time course of hypothalamic CRF activity after the administration of two different stresses. The time course of hypothalamic corticotropin-releasing factor (CRF) activity after the administration of ether stress was different from that after immobilization stress. The maximal response CRF activity was observed 2 min after ether stress, followed by a precipitous decrease 5 min after the stress. A gradual increase of CRF activity was subsequently observed for several minutes with fluctuated changes. Thus, response pattern was vibratory. But under immobilization stress, markedly fluctuant changes of CRF activity seen in the case of ether stress did not appear after the maximal response observed at 2 min, indicating that the response pattern was not vibratory. On the other hand, the concentration of plasma corticosterone increased significantly 5 min after the ether or immobilization stress with the peak value around 17 min."}
{"id": "PMID:1087605", "title": "Deoxyribonucleotide pools in mouse-fibroblast cell lines with altered ribonucleotide reductase.", "content": "Mutant cells lines of 3T6 mouse fibroblasts, resistant to thymidine and deoxyadenosine, have an altered allosteric regulation of the enzyme ribonucleotide reductase (Meuth, M. and Green, H., Cell, 3, 367, 1974). Compared to 3T6, these lines contain larger pools of deoxynucleoside triphosphates, in particular deoxycytidine triphosphate, but show a normal rate of DNA synthesis. Addition of thymidine or deoxyadenosine to 3T6 cells results in large accumulations of the corresponding triphosphates and a dramatic decrease in the dCTP pool, concomitant with inhibition of DNA synthesis. Addition of thymidine to the mutant cell lines also leads to an increase in the dTTP pool but does not result in a depletion of dCTP or inhibition of DNA synthesis. Addition of deoxyadenosine only leads to a small increase of the dATP pool. In general the change in the allosteric regulation of bibonucleotide reductase is reflected in the deoxynucleotide pools.", "contents": "Deoxyribonucleotide pools in mouse-fibroblast cell lines with altered ribonucleotide reductase. Mutant cells lines of 3T6 mouse fibroblasts, resistant to thymidine and deoxyadenosine, have an altered allosteric regulation of the enzyme ribonucleotide reductase (Meuth, M. and Green, H., Cell, 3, 367, 1974). Compared to 3T6, these lines contain larger pools of deoxynucleoside triphosphates, in particular deoxycytidine triphosphate, but show a normal rate of DNA synthesis. Addition of thymidine or deoxyadenosine to 3T6 cells results in large accumulations of the corresponding triphosphates and a dramatic decrease in the dCTP pool, concomitant with inhibition of DNA synthesis. Addition of thymidine to the mutant cell lines also leads to an increase in the dTTP pool but does not result in a depletion of dCTP or inhibition of DNA synthesis. Addition of deoxyadenosine only leads to a small increase of the dATP pool. In general the change in the allosteric regulation of bibonucleotide reductase is reflected in the deoxynucleotide pools."}
{"id": "PMID:1087607", "title": "Visual-vestibular interactions in the vestibular nuclei of the goldfish.", "content": "The responses of vestibular nuclei neurons of relaxed unanaesthetized goldfish have been examined with trapezoid velocity stimuli under three conditions. Responses to horizontal body rotation in the dark (pure vestibular stimulation) resemble those observed in vestibular nerve afferents. Optokinetic responses to exclusive visual surround-motion are also direction-specific and, in contrast to vestibular responses, exhibit a tonic response to constant velocity. They show three different response profiles, classified A,B or C, based on the neuron's discharge rate: either increasing, decreasing or remaining constant once surround motion is maintained at constant velocity. Following these dynamic effects, optokinetic responses have a maintained modulation of resting discharge until deceleration commences. The time constants associated with the dynamic effects vary between 1 and 11 seconds. Steady-state modulation of optokinetic responses shows a weak relation to stimulus velocities exceeding 10 deg/sec. Responses to body rotation in the light were found to linearly combine the weighted vestibular and optokinetic responses so that accurate velocity information is available for sensory and motor functions independent of the neuron's vestibular (I,II) or optokinetic (A,B,C) response type. The principle of this visual-vestibular interaction is discussed with respect to multisensory processing within the vestibular nuclei.", "contents": "Visual-vestibular interactions in the vestibular nuclei of the goldfish. The responses of vestibular nuclei neurons of relaxed unanaesthetized goldfish have been examined with trapezoid velocity stimuli under three conditions. Responses to horizontal body rotation in the dark (pure vestibular stimulation) resemble those observed in vestibular nerve afferents. Optokinetic responses to exclusive visual surround-motion are also direction-specific and, in contrast to vestibular responses, exhibit a tonic response to constant velocity. They show three different response profiles, classified A,B or C, based on the neuron's discharge rate: either increasing, decreasing or remaining constant once surround motion is maintained at constant velocity. Following these dynamic effects, optokinetic responses have a maintained modulation of resting discharge until deceleration commences. The time constants associated with the dynamic effects vary between 1 and 11 seconds. Steady-state modulation of optokinetic responses shows a weak relation to stimulus velocities exceeding 10 deg/sec. Responses to body rotation in the light were found to linearly combine the weighted vestibular and optokinetic responses so that accurate velocity information is available for sensory and motor functions independent of the neuron's vestibular (I,II) or optokinetic (A,B,C) response type. The principle of this visual-vestibular interaction is discussed with respect to multisensory processing within the vestibular nuclei."}
{"id": "PMID:1087608", "title": "Experimental allergic encephalomyelitis.-- The effect of dexamethasone on growth and proliferation in the draining lymph node.", "content": "Dexamethasone, when administered from day 0 to day 9 following the sensitizing injection of encephalitogenic protein in complete Freund's adjuvant, suppressed both the characteristic enlargement of the paracortical area of the draining lymph node and the development of clinical and histopathological signs of experimental allergic encephalomyelitis. Division of cells within the node was not inhibited and therefore the effect of dexamethasone appeared to be a consequence of reduced migration of lymphocytes into the node.", "contents": "Experimental allergic encephalomyelitis.-- The effect of dexamethasone on growth and proliferation in the draining lymph node. Dexamethasone, when administered from day 0 to day 9 following the sensitizing injection of encephalitogenic protein in complete Freund's adjuvant, suppressed both the characteristic enlargement of the paracortical area of the draining lymph node and the development of clinical and histopathological signs of experimental allergic encephalomyelitis. Division of cells within the node was not inhibited and therefore the effect of dexamethasone appeared to be a consequence of reduced migration of lymphocytes into the node."}
{"id": "PMID:1087609", "title": "[Driving phenomenon in neurons of the spinal center of frog lymph hearts].", "content": "Bioelectrical activity of motor axons of the lower lymphatic hearts was recorded in frogs. When the 5th and 6th dorsal roots were stimulated at a frequency lower than the spontaneous frequency, artificial bursts of motor impulses were usually produced together with spontaneous impulses. Latency of artificial impulses depended upon the time of stimulation in the interburst interval. When the same roots were stimulated at a frequency higher than the spontaneous rate, only motor impulses corresponding to the artificial frequency were observed. The latency of these artificial impulses was 100--160 msec.", "contents": "[Driving phenomenon in neurons of the spinal center of frog lymph hearts]. Bioelectrical activity of motor axons of the lower lymphatic hearts was recorded in frogs. When the 5th and 6th dorsal roots were stimulated at a frequency lower than the spontaneous frequency, artificial bursts of motor impulses were usually produced together with spontaneous impulses. Latency of artificial impulses depended upon the time of stimulation in the interburst interval. When the same roots were stimulated at a frequency higher than the spontaneous rate, only motor impulses corresponding to the artificial frequency were observed. The latency of these artificial impulses was 100--160 msec."}
{"id": "PMID:1087610", "title": "[Possible participation of lactic acid in formation of a non-mediator agent stimulating the heart during exposure of the myocardium to sympathetic influences].", "content": "Release of A-factor, the physiologically active substance of a non--mediatory nature under sympathetic stimulation, depends upon the glycolytic process. To examine the assumption that A-factor may be a glycolytic product, lactic acid was studied in particular, and it was shown that A-factor is not identical to lactic acid. However it was found out that lactic acid participates in the release of A-factor in the heart. It was shown that under the effect of lactic acid a substance having high cardiostimulating activity was released in the frog heart muscle. This cardiostimulating substance as well as A-factor increases the amplitude of contractions of A-factor, was not abolished with beta-sympathicolytic inderal. The ability of both substances to stimulate heart activity was preserved after 20 min heating in a water bath. The cardiostimulating substance similar to A-factor increases heart sensitivity to sympathetic and decreases it to parasympathetic stimulations. It may be suggested that the cardiostimulating substance released in the heart under the effect of lactic acid is identical to A-factor released in the myocardium under the action of catecholamines and stimulation of the sympathetic nerve.", "contents": "[Possible participation of lactic acid in formation of a non-mediator agent stimulating the heart during exposure of the myocardium to sympathetic influences]. Release of A-factor, the physiologically active substance of a non--mediatory nature under sympathetic stimulation, depends upon the glycolytic process. To examine the assumption that A-factor may be a glycolytic product, lactic acid was studied in particular, and it was shown that A-factor is not identical to lactic acid. However it was found out that lactic acid participates in the release of A-factor in the heart. It was shown that under the effect of lactic acid a substance having high cardiostimulating activity was released in the frog heart muscle. This cardiostimulating substance as well as A-factor increases the amplitude of contractions of A-factor, was not abolished with beta-sympathicolytic inderal. The ability of both substances to stimulate heart activity was preserved after 20 min heating in a water bath. The cardiostimulating substance similar to A-factor increases heart sensitivity to sympathetic and decreases it to parasympathetic stimulations. It may be suggested that the cardiostimulating substance released in the heart under the effect of lactic acid is identical to A-factor released in the myocardium under the action of catecholamines and stimulation of the sympathetic nerve."}
{"id": "PMID:1087611", "title": "[Rsponses of the primary afferents of the frog lateral semicircular canal to caloric stimuli following section of the eighth nerve].", "content": "In curarized Rana temporaria, after transection of the 8th nerve, responses of 75 primary afferents of the LSC nerve to short local caloric stimule (0.5--12 degrees C over the canal temperature) creating theexcitatory current of LSC endolymph, were studied. In 41 per cent of the units (group A) the maximal (peak) frequencies (PF) of responses were shown to be associated either by linear or semilograithmic relations with the peak temperature (PT). In 23 per cent of the units (group B), PFPS revealed saturation at PT 8-12 degrees C. In 29  per cent of the units (group C), PFs were associated with PTs first by direct and then -- at increasing PTs-- by reverse proportionality. Duration of group A responses was related to PTs either in linear or semilogarithmic way. Comparison with previous data obtained in identical conditions but with intact 8th nerve indicates no noticeable difference in responses where efferent control was excluded.", "contents": "[Rsponses of the primary afferents of the frog lateral semicircular canal to caloric stimuli following section of the eighth nerve]. In curarized Rana temporaria, after transection of the 8th nerve, responses of 75 primary afferents of the LSC nerve to short local caloric stimule (0.5--12 degrees C over the canal temperature) creating theexcitatory current of LSC endolymph, were studied. In 41 per cent of the units (group A) the maximal (peak) frequencies (PF) of responses were shown to be associated either by linear or semilograithmic relations with the peak temperature (PT). In 23 per cent of the units (group B), PFPS revealed saturation at PT 8-12 degrees C. In 29  per cent of the units (group C), PFs were associated with PTs first by direct and then -- at increasing PTs-- by reverse proportionality. Duration of group A responses was related to PTs either in linear or semilogarithmic way. Comparison with previous data obtained in identical conditions but with intact 8th nerve indicates no noticeable difference in responses where efferent control was excluded."}
{"id": "PMID:1087612", "title": "[Effect of thrombin on chemoreceptors of the frog carotid labyrinth].", "content": "The frog's electrical activity of the carotid body chemoreceptors reacting to thrombin was studied. The chemoreceptor response was estimated by means of afferent impulsation in the n. glossopharingeus. The afferent impulsation frequency increases to a certain extent in proportion to the irritant concentration; too high a thrombin concentration is able to suppress the chemoreceptor activity. The direct dependence between the receptor response and the effector reaction diminishing the blood coagulation, was established. The receptors adapt slowly, their discharge is of an irregular character.", "contents": "[Effect of thrombin on chemoreceptors of the frog carotid labyrinth]. The frog's electrical activity of the carotid body chemoreceptors reacting to thrombin was studied. The chemoreceptor response was estimated by means of afferent impulsation in the n. glossopharingeus. The afferent impulsation frequency increases to a certain extent in proportion to the irritant concentration; too high a thrombin concentration is able to suppress the chemoreceptor activity. The direct dependence between the receptor response and the effector reaction diminishing the blood coagulation, was established. The receptors adapt slowly, their discharge is of an irregular character."}
{"id": "PMID:1087613", "title": "[Aorto-coronary by-pass. Personal experience based on 342 cases operated on between April 1971 and December 1974 (author's transl)].", "content": "The authors report on the casuistry of aorto-coronary by-pass operations they performed between April 1971 and December 1974, discussing the criteria which indicate the necessity of operating, the principles of the operative techniques, and the results obtained. Out of a total of 342 operated cases, there was an operative mortality (within 30 days) of 3% in isolated by-pass cases, and 4% if one includes associated operations. In 3,5% of patients, postoperative necrosis was present. Almost the same values were found in the smaller group (66 cases) which underwent urgent by-pass operations. The authors consider that the aorto-coronary by-pass operation is a very useful treatment, both for stable and unstable angina, as long as the anatomo-functional conditions exist.", "contents": "[Aorto-coronary by-pass. Personal experience based on 342 cases operated on between April 1971 and December 1974 (author's transl)]. The authors report on the casuistry of aorto-coronary by-pass operations they performed between April 1971 and December 1974, discussing the criteria which indicate the necessity of operating, the principles of the operative techniques, and the results obtained. Out of a total of 342 operated cases, there was an operative mortality (within 30 days) of 3% in isolated by-pass cases, and 4% if one includes associated operations. In 3,5% of patients, postoperative necrosis was present. Almost the same values were found in the smaller group (66 cases) which underwent urgent by-pass operations. The authors consider that the aorto-coronary by-pass operation is a very useful treatment, both for stable and unstable angina, as long as the anatomo-functional conditions exist."}
{"id": "PMID:1087614", "title": "[Operative and long term results in 52 patients operated on for chronic postinfarction left ventricular aneurysm (author's transl)].", "content": "Operative and late post-operative results in 52 patients undergoing operation for chronic postinfarction left ventricular aneurysm are described. Aneurysm resection was performed in 33 patients. This procedure was combined with myocardial revascularization by means of insertion of a venous aorto-coronary by-pass graft in 14 cases. Hospital mortality for the total group was 3,8% (2 deaths). Surviving patients were followed-up 3 to 66 months. Two late deaths occurred. Clinical and functinal improvement occurred in the majority of surviving patients. The impact of simultaneous aorto-coronary by-pass grafting on operative results is discussed.", "contents": "[Operative and long term results in 52 patients operated on for chronic postinfarction left ventricular aneurysm (author's transl)]. Operative and late post-operative results in 52 patients undergoing operation for chronic postinfarction left ventricular aneurysm are described. Aneurysm resection was performed in 33 patients. This procedure was combined with myocardial revascularization by means of insertion of a venous aorto-coronary by-pass graft in 14 cases. Hospital mortality for the total group was 3,8% (2 deaths). Surviving patients were followed-up 3 to 66 months. Two late deaths occurred. Clinical and functinal improvement occurred in the majority of surviving patients. The impact of simultaneous aorto-coronary by-pass grafting on operative results is discussed."}
{"id": "PMID:1087617", "title": "Mitotic variations in the lens epithelium of the frog IV. Studies with isolated anuran pituitary factors.", "content": "Proteins corresponding to growth hormone and prolactin have been electrophoretically separated from the pituitary gland of the frog (Rana pipiens berlandieri and Rana catesbeiana). These substances can independently stimulate mitosis in the lenses of animals where it had previously been inhibited by means of hypophysectomy. DNA synthesis is greatly augmented six days after the start of hormone administration to intact animals. Nine days are required before this occurs in hypophysectomized ones. No other anterior pituitary hormone has been found to achieve the effect described. Initial attempts at amino acid analysis, though incomplete, indicate that the two frog hormones are different from each other as well as from their bovine counterparts. Pituitary factors do not affect mitotic activity in either corneal epi or endothelium of the frog.", "contents": "Mitotic variations in the lens epithelium of the frog IV. Studies with isolated anuran pituitary factors. Proteins corresponding to growth hormone and prolactin have been electrophoretically separated from the pituitary gland of the frog (Rana pipiens berlandieri and Rana catesbeiana). These substances can independently stimulate mitosis in the lenses of animals where it had previously been inhibited by means of hypophysectomy. DNA synthesis is greatly augmented six days after the start of hormone administration to intact animals. Nine days are required before this occurs in hypophysectomized ones. No other anterior pituitary hormone has been found to achieve the effect described. Initial attempts at amino acid analysis, though incomplete, indicate that the two frog hormones are different from each other as well as from their bovine counterparts. Pituitary factors do not affect mitotic activity in either corneal epi or endothelium of the frog."}
{"id": "PMID:1087619", "title": "Cytologie and cytochemistry of colony cells in soft agar gel culture from normal and leukemic bone marrow.", "content": "In order to judge differentiation of cells in soft agar colonies, cytological and cytochemical classification of single cells within these colonies is necessary. In this study, 1,026 colonies from 15 normal and 95 leukemic bone marrows have been evaluated using cytological, cytochemical, and immunocytochemical techniques. In 180 colonies from 15 normal controls no segmented neutrophils have been observed. The colonies mostly consisted of monocytes and macrophages, rarely pure eosinophil colonies were observed. The number of monocyte/macrophage colonies in untreated AML and the percentage of pure eosinophil colonies in AML and ALL in remission are reduced, as compared to normal controls. In 174 colonies from a total of 926 colonies derived from bone marrows of leukemic patients, plasma cells and in 20 colonies, blast cells have been observed. In contrast to normal colonies, growth of colonies containing blast cells does not depend upon the conditioned medium of the leukocyte feederlayer. This investigation has demonstrated the necessity of cytological and cytochemical classification in addition to quantiative evaluation of soft agar colonies when studying the effect of factors on proliferation and differentiation of normal and leukemic stem cells.", "contents": "Cytologie and cytochemistry of colony cells in soft agar gel culture from normal and leukemic bone marrow. In order to judge differentiation of cells in soft agar colonies, cytological and cytochemical classification of single cells within these colonies is necessary. In this study, 1,026 colonies from 15 normal and 95 leukemic bone marrows have been evaluated using cytological, cytochemical, and immunocytochemical techniques. In 180 colonies from 15 normal controls no segmented neutrophils have been observed. The colonies mostly consisted of monocytes and macrophages, rarely pure eosinophil colonies were observed. The number of monocyte/macrophage colonies in untreated AML and the percentage of pure eosinophil colonies in AML and ALL in remission are reduced, as compared to normal controls. In 174 colonies from a total of 926 colonies derived from bone marrows of leukemic patients, plasma cells and in 20 colonies, blast cells have been observed. In contrast to normal colonies, growth of colonies containing blast cells does not depend upon the conditioned medium of the leukocyte feederlayer. This investigation has demonstrated the necessity of cytological and cytochemical classification in addition to quantiative evaluation of soft agar colonies when studying the effect of factors on proliferation and differentiation of normal and leukemic stem cells."}
{"id": "PMID:1087620", "title": "Immunological characterization of blast cells in patients with acute lymphoblastic leukemia. Evaluation of its clinical significance.", "content": "A panel of lymphocyte surface markers was used to identify blast cells from 111 patients with acute lymphoblastic leukemia (ALL). Three groups of patients were found. 1) 14 patients with B derived ALL. Only three patients had a common ALL; in the other cases the blastic proliferation was featured by Burkitt's tumor cells or supervened in patients affected with chronic lymphocytic leukemia (CLL). 2) The blast cells from 28% of the patients with common ALL had T cell properties. 3) The cells from the largest group of patients did not bear B or T cell markers but were featured by the presence of a leukemia-associated antigen revealed by a rabbit antiserum to CLL B cells. Studies with another antiserum to CLL B cells as well as with an antiserum to foetal thymocytes revealed also leukemia-associated antigens but these antigenic determinants were present on all acute leukemia cells which had been tested and were therefore of no help to classify various leukemias. A number of clinical and hematological findings were more frequent in the group of patients with T cell ALL: high white blood cell counts, tumoral disease, thymic enlargement, meningeal involvement, strong acid phosphatase activity in blast cells. However no difference in the survival curve is yet apparent at 30 months.", "contents": "Immunological characterization of blast cells in patients with acute lymphoblastic leukemia. Evaluation of its clinical significance. A panel of lymphocyte surface markers was used to identify blast cells from 111 patients with acute lymphoblastic leukemia (ALL). Three groups of patients were found. 1) 14 patients with B derived ALL. Only three patients had a common ALL; in the other cases the blastic proliferation was featured by Burkitt's tumor cells or supervened in patients affected with chronic lymphocytic leukemia (CLL). 2) The blast cells from 28% of the patients with common ALL had T cell properties. 3) The cells from the largest group of patients did not bear B or T cell markers but were featured by the presence of a leukemia-associated antigen revealed by a rabbit antiserum to CLL B cells. Studies with another antiserum to CLL B cells as well as with an antiserum to foetal thymocytes revealed also leukemia-associated antigens but these antigenic determinants were present on all acute leukemia cells which had been tested and were therefore of no help to classify various leukemias. A number of clinical and hematological findings were more frequent in the group of patients with T cell ALL: high white blood cell counts, tumoral disease, thymic enlargement, meningeal involvement, strong acid phosphatase activity in blast cells. However no difference in the survival curve is yet apparent at 30 months."}
{"id": "PMID:1087621", "title": "Immunological membrane markers of Hodgkin's cells.", "content": "Reed-Sternberg and other Hodgkin's giant cells derived from involved spleens and lymph nodes of patients with Hodgkin's disease were examined for surface markers of T and B cells and macrophages. Attachment and phagocytosis of untreated (E) or sensitized (EA and EAC) sheep red blood cells and yeast by Reed-Sternberg cells did not occur. IgG frequently detected at the membrane of Reed-Sternberg cells was partially removed by incubation and washing at 37 degrees C. Fluorescence with a specific anti-T cell serum was not seen on Reed-Sternberg and other Hodgkin's giant cells lack most detectable normal human lymphoid cell markers, but do exhibit membrane bound immunoglobulin possibly of exogenous origin.", "contents": "Immunological membrane markers of Hodgkin's cells. Reed-Sternberg and other Hodgkin's giant cells derived from involved spleens and lymph nodes of patients with Hodgkin's disease were examined for surface markers of T and B cells and macrophages. Attachment and phagocytosis of untreated (E) or sensitized (EA and EAC) sheep red blood cells and yeast by Reed-Sternberg cells did not occur. IgG frequently detected at the membrane of Reed-Sternberg cells was partially removed by incubation and washing at 37 degrees C. Fluorescence with a specific anti-T cell serum was not seen on Reed-Sternberg and other Hodgkin's giant cells lack most detectable normal human lymphoid cell markers, but do exhibit membrane bound immunoglobulin possibly of exogenous origin."}
{"id": "PMID:1087622", "title": "The phenotypic abnormality in leukemia: a defective cell-factor interaction?", "content": "Differentiation of hemopoietic cells appears to depend upon specific interactions of certain cell-factors. The phenotypic abnormality in leukemia may involve an impairment in these interactions. In this report we present some of our views of leukemogenesis with respect to cell-factor interaction and the feasibility of experimental approaches to this problem. In culture, the interaction of myelogenous cells with factor(s) leading to differentiation can be measured either with a suspension mass culture method or by a solid (semi-soft) clonal method. The protein factors that support the growth of hemopoietic cells in suspension culture are termed growth stimulating factors (GSA) and in semi-solid culture, colony stimulating factors (CSA). Studies using conditioned medium prepared from phytohemagglutinin stimulated human lymphocytes (PHA-LyCM) and whole human embryo cells (WHE) revealed that GSA and CSA were not identical for growth of either normal human or leukemic leukocytes. In some cases maturation of leukemic leukocytes was observed. Fractionation of PHA-LyCM showed that there are three peaks for CSA. Each peak contains different fractions for supporting cellular proliferation, differentiation, and self-renewal of precursor cells in suspension culture. Apparently, each contains heterogenous species of protein factors some of which functionally overlap, while others do not.", "contents": "The phenotypic abnormality in leukemia: a defective cell-factor interaction? Differentiation of hemopoietic cells appears to depend upon specific interactions of certain cell-factors. The phenotypic abnormality in leukemia may involve an impairment in these interactions. In this report we present some of our views of leukemogenesis with respect to cell-factor interaction and the feasibility of experimental approaches to this problem. In culture, the interaction of myelogenous cells with factor(s) leading to differentiation can be measured either with a suspension mass culture method or by a solid (semi-soft) clonal method. The protein factors that support the growth of hemopoietic cells in suspension culture are termed growth stimulating factors (GSA) and in semi-solid culture, colony stimulating factors (CSA). Studies using conditioned medium prepared from phytohemagglutinin stimulated human lymphocytes (PHA-LyCM) and whole human embryo cells (WHE) revealed that GSA and CSA were not identical for growth of either normal human or leukemic leukocytes. In some cases maturation of leukemic leukocytes was observed. Fractionation of PHA-LyCM showed that there are three peaks for CSA. Each peak contains different fractions for supporting cellular proliferation, differentiation, and self-renewal of precursor cells in suspension culture. Apparently, each contains heterogenous species of protein factors some of which functionally overlap, while others do not."}
{"id": "PMID:1087623", "title": "Prognostic criteria and staging in ALL in childhood.", "content": "On the basis of a retrospective review of 100 children with acute non-myeloid leukaemia the authors examined the effect on the prognosis of some initial features such as age, nodal and organ enlargement, initial white blood cell count, blast morphology and cytochemical characteristics, and platelet count. A remarkable correlation was found between age at diagnosis, initial white blood cell count and platelet count, and total survival and duration of remission. There was no significant correlation between PAS score and prognosis. The beta-glucoronidase reaction did not prove a useful index of prognosis. Adverse prognostic factors were an age below 2 or one above 10 years, a high initial white blood cell count and a low platelet count. The usefulness of attempts at staging in acute lymphoblastic leukaemia in childhood are discussed together with the predictive value of some features present at disgnosis.", "contents": "Prognostic criteria and staging in ALL in childhood. On the basis of a retrospective review of 100 children with acute non-myeloid leukaemia the authors examined the effect on the prognosis of some initial features such as age, nodal and organ enlargement, initial white blood cell count, blast morphology and cytochemical characteristics, and platelet count. A remarkable correlation was found between age at diagnosis, initial white blood cell count and platelet count, and total survival and duration of remission. There was no significant correlation between PAS score and prognosis. The beta-glucoronidase reaction did not prove a useful index of prognosis. Adverse prognostic factors were an age below 2 or one above 10 years, a high initial white blood cell count and a low platelet count. The usefulness of attempts at staging in acute lymphoblastic leukaemia in childhood are discussed together with the predictive value of some features present at disgnosis."}
{"id": "PMID:1087626", "title": "alpha1-antitrypsin: common subtypes of Pi M.", "content": "More than 20 different alleles are so far known at the Pi locus, corresponding to a total variant phenotype frequency of about 10% in most western Europeans. The common phenotype Pi M constitutes the remaining major group. Now it has been possible to identify three subtypes M1, M1M2 and M2, corresponding to the gene products of two common alleles PiM1 and PiM2, segregating as autosomal codominant alleles. Preliminary gene frequencies are reported for eight populations, the PiM2 frequency varying from 0.20 in Maris (USSR) to 0.02 in Bantus (Kenya).", "contents": "alpha1-antitrypsin: common subtypes of Pi M. More than 20 different alleles are so far known at the Pi locus, corresponding to a total variant phenotype frequency of about 10% in most western Europeans. The common phenotype Pi M constitutes the remaining major group. Now it has been possible to identify three subtypes M1, M1M2 and M2, corresponding to the gene products of two common alleles PiM1 and PiM2, segregating as autosomal codominant alleles. Preliminary gene frequencies are reported for eight populations, the PiM2 frequency varying from 0.20 in Maris (USSR) to 0.02 in Bantus (Kenya)."}
{"id": "PMID:1087627", "title": "Cellular changes in cultured lenses.", "content": "Observations were made on the frog lens epithelium after culture of the entire lens or of capsular explants. General deviations from normal lens structure as well as specific changes in two media were studied. DNA synthesis and mitosis were induced in the central epithelial cells. Disruption of the orderly, single, epithelial layer that is characteristic of normal lenses was accompanied by the appearance of multilayered plaques of epithelial cells and invasion of vacuolated regions of the lens fibers by epithelial cells. Cells that are fibroblast-like in appearance were observed in regions of the capsule depleted of cells and at the free edges of epithelial sheets in cell culture. Epithelial cells were surrounded by capsule-like material even situated in the lens interior. Nuclie derived from central epithelial cells of lenses cultured in L-15 medium and medium 199 had served as donors in previous nuclear transfer experiments in this laboratory. In our current observation of L-15-cultured lenses, cells were sparsely distributed on the capsule and nuclei were abnormally shaped; in 199-cultured lenses, cells were more densely distributed and nuclei resembled those of normal lenses. Medium 199 without serum could better maintain normal lens structure than L-15 medium without serum. In addition, the percentage of epithelial explants demonstrating cellular outgrowth was greater in medium 199. The differences in cellular behavior were shown not to be the result of different sugars, pH, or the presence of CO2. The nuclear transfer results may reflect the structural changes in the epithelium after lens culture in the two media.", "contents": "Cellular changes in cultured lenses. Observations were made on the frog lens epithelium after culture of the entire lens or of capsular explants. General deviations from normal lens structure as well as specific changes in two media were studied. DNA synthesis and mitosis were induced in the central epithelial cells. Disruption of the orderly, single, epithelial layer that is characteristic of normal lenses was accompanied by the appearance of multilayered plaques of epithelial cells and invasion of vacuolated regions of the lens fibers by epithelial cells. Cells that are fibroblast-like in appearance were observed in regions of the capsule depleted of cells and at the free edges of epithelial sheets in cell culture. Epithelial cells were surrounded by capsule-like material even situated in the lens interior. Nuclie derived from central epithelial cells of lenses cultured in L-15 medium and medium 199 had served as donors in previous nuclear transfer experiments in this laboratory. In our current observation of L-15-cultured lenses, cells were sparsely distributed on the capsule and nuclei were abnormally shaped; in 199-cultured lenses, cells were more densely distributed and nuclei resembled those of normal lenses. Medium 199 without serum could better maintain normal lens structure than L-15 medium without serum. In addition, the percentage of epithelial explants demonstrating cellular outgrowth was greater in medium 199. The differences in cellular behavior were shown not to be the result of different sugars, pH, or the presence of CO2. The nuclear transfer results may reflect the structural changes in the epithelium after lens culture in the two media."}
{"id": "PMID:1087632", "title": "Some biochemical changes in lenses of riboflavin deficient rats.", "content": "In order to better understand the role of riboflavin in the lens, we investigated the lenses of rats fed on a riboflavin deficient diet for 7 weeks to determine changes in the inactivation of cortisol, the glucose-6-phosphate dehydrogenase (G-6-PD) activity and the histidine or 5-hydroxytryptophan decarboxylase activity. The cortisol-binding capacity and G-6-PD activity in the lenses of rats fed on a riboflavin deficient diet were found to be decreased as compared with a control. Histidine decarboxylase activity was found twice as high in the riboflavin deficient rats as in the normal lenses however, 5-hydroxytryptophan decarboxylase was somewhat decreased. These findings show that catabolical changes in the metabolism of rat lenses are induced comprehensively by feeding on a riboflavin deficient diet in the same manner as changes are seen in the cataractous lens.", "contents": "Some biochemical changes in lenses of riboflavin deficient rats. In order to better understand the role of riboflavin in the lens, we investigated the lenses of rats fed on a riboflavin deficient diet for 7 weeks to determine changes in the inactivation of cortisol, the glucose-6-phosphate dehydrogenase (G-6-PD) activity and the histidine or 5-hydroxytryptophan decarboxylase activity. The cortisol-binding capacity and G-6-PD activity in the lenses of rats fed on a riboflavin deficient diet were found to be decreased as compared with a control. Histidine decarboxylase activity was found twice as high in the riboflavin deficient rats as in the normal lenses however, 5-hydroxytryptophan decarboxylase was somewhat decreased. These findings show that catabolical changes in the metabolism of rat lenses are induced comprehensively by feeding on a riboflavin deficient diet in the same manner as changes are seen in the cataractous lens."}
{"id": "PMID:1087635", "title": "Epidemiologic characteristics of women infected with Corynebacterium vaginale (Haemophilus vainalis).", "content": "In a group of 184 women infected with Corynebacterium vaginale (Haemophilus vaginalis), 34% over age 30 were divorced or separated and 8% gave a history of induced abortion. Fifty-one percent were taking an oral contraceptive drug, as compared to 36% of 140 women in a control group. Various sexually transmitted diseases were diagnosed either concomitantly or at another time in 52% of women in the study group and 38% of those in the control group. The rate of cervical neoplasia (invasive carcinoma, carcinoma in situ, and dysplasia) was 13.6% in the study group and 5.7% in the control group, the rate in the study group being several times that in the general population. These and other available epidemiologic data support the conclusion that C vainale is transmitted sexually.", "contents": "Epidemiologic characteristics of women infected with Corynebacterium vaginale (Haemophilus vainalis). In a group of 184 women infected with Corynebacterium vaginale (Haemophilus vaginalis), 34% over age 30 were divorced or separated and 8% gave a history of induced abortion. Fifty-one percent were taking an oral contraceptive drug, as compared to 36% of 140 women in a control group. Various sexually transmitted diseases were diagnosed either concomitantly or at another time in 52% of women in the study group and 38% of those in the control group. The rate of cervical neoplasia (invasive carcinoma, carcinoma in situ, and dysplasia) was 13.6% in the study group and 5.7% in the control group, the rate in the study group being several times that in the general population. These and other available epidemiologic data support the conclusion that C vainale is transmitted sexually."}
{"id": "PMID:1087637", "title": "Studies on urinary kallikreins. I. Purification and characterization of human urinary kallikreins.", "content": "Human urinary kallikrein [EC 3.4.21.8] (HUK) was purified about 200-fold with an overall yield of 40 percent from crude powder by DEAE-cellulose chromatography, acetone fractionation, Sephadex G-100 gel filtration and DEAE-Sephadex A-50 chromatography. Its activity was 200 kallikrein units (KU) per A280. HUK from active fractions obtained by DEAE-Sephadex A-50 chromatography was separated into three active components showing isoelectric points of 3.9 (HUK-1), 4.0 (HUK-2), and 4.2 (HUK-3) by isoelectric focusing: each HUK component was homogeneous on disc electrophoresis. The approximate molecular weights of HUK-1, -2 and -3 were estimated to be 2.7 X 10(4), 2.7 X 10(4), and 2.9 X 10(4), respectively, by gel filtration on a Sephadex G-100 column. The optimum pH's of HUK-1, -2, and -3 in esterolytic action were found to be 8.0, 8.3, and 7.5, respectively, and they were fairly heat stable in comparison with other glandular kallikreins. The three components of HUK were weakly inhibited by Trasylol, but were not affected by soybean and ovomucoid trypsin inhibitors. They were strongly resistant to treatment with urea and weakly resistant to treatment with guanidine. The activation energies of HUK-1, -2, and -3 were found to by 1.17 X 10(4), 5.1 X 10(3), and 1.45 X 10(4) cal per mole, respectively. The Km values were estimated toward N-alpha-tosyl-L-arginine methyl ester (TAME), N-alpha-benozyl-L-arginine ethyl ester (BAEE), and N-alpha-benozyl-L-arginine methyl ester (BAME).", "contents": "Studies on urinary kallikreins. I. Purification and characterization of human urinary kallikreins. Human urinary kallikrein [EC 3.4.21.8] (HUK) was purified about 200-fold with an overall yield of 40 percent from crude powder by DEAE-cellulose chromatography, acetone fractionation, Sephadex G-100 gel filtration and DEAE-Sephadex A-50 chromatography. Its activity was 200 kallikrein units (KU) per A280. HUK from active fractions obtained by DEAE-Sephadex A-50 chromatography was separated into three active components showing isoelectric points of 3.9 (HUK-1), 4.0 (HUK-2), and 4.2 (HUK-3) by isoelectric focusing: each HUK component was homogeneous on disc electrophoresis. The approximate molecular weights of HUK-1, -2 and -3 were estimated to be 2.7 X 10(4), 2.7 X 10(4), and 2.9 X 10(4), respectively, by gel filtration on a Sephadex G-100 column. The optimum pH's of HUK-1, -2, and -3 in esterolytic action were found to be 8.0, 8.3, and 7.5, respectively, and they were fairly heat stable in comparison with other glandular kallikreins. The three components of HUK were weakly inhibited by Trasylol, but were not affected by soybean and ovomucoid trypsin inhibitors. They were strongly resistant to treatment with urea and weakly resistant to treatment with guanidine. The activation energies of HUK-1, -2, and -3 were found to by 1.17 X 10(4), 5.1 X 10(3), and 1.45 X 10(4) cal per mole, respectively. The Km values were estimated toward N-alpha-tosyl-L-arginine methyl ester (TAME), N-alpha-benozyl-L-arginine ethyl ester (BAEE), and N-alpha-benozyl-L-arginine methyl ester (BAME)."}
{"id": "PMID:1087638", "title": "Laboratory diagnosis of rheumatoid arthritis: a solid phase radioassay for IgG and IgM antiglobulins.", "content": "A technique suitable for the routine estimation of IgM and IgG antiglobulins has been devised. The assay involves the binding of antiglobulins to plastic tubes coated with rabbit immunoglobulin: the amount of antiglobulin bound is then determined by adding radiolabelled antihuman IgG or IgM. The conditions for the assay have been examined and optimal incubation times and amounts of reagents established. Verification of the antibody nature of antiglobulin activity has been obtained. Both IgG and IgM antiglobulins were raised in virtually all seropositive rheumatoid arthritics, and most seronegative patients gave elevated values for either IgM or IgG rheumatoid factors. The use of an anti-light chain reagent as a screening test for total antiglobulins was investigated. These tests should prove valuable in diagnosis and permit quantitative evaluation of research studies.", "contents": "Laboratory diagnosis of rheumatoid arthritis: a solid phase radioassay for IgG and IgM antiglobulins. A technique suitable for the routine estimation of IgM and IgG antiglobulins has been devised. The assay involves the binding of antiglobulins to plastic tubes coated with rabbit immunoglobulin: the amount of antiglobulin bound is then determined by adding radiolabelled antihuman IgG or IgM. The conditions for the assay have been examined and optimal incubation times and amounts of reagents established. Verification of the antibody nature of antiglobulin activity has been obtained. Both IgG and IgM antiglobulins were raised in virtually all seropositive rheumatoid arthritics, and most seronegative patients gave elevated values for either IgM or IgG rheumatoid factors. The use of an anti-light chain reagent as a screening test for total antiglobulins was investigated. These tests should prove valuable in diagnosis and permit quantitative evaluation of research studies."}
{"id": "PMID:1087634", "title": "Freeze-fracture-autoradiography.", "content": "A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts.", "contents": "Freeze-fracture-autoradiography. A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing. The advantages and limitations of the new method are discussed in terms of monolayer quality, contact, histochemography, resolution, freezing and recrystallisation artefacts."}
{"id": "PMID:1087639", "title": "Failure to suppress theta antigenic expression in progeny derived from pre-immunized maternal recipients.", "content": "One possible theory concerning the success of the fetus as an allograft has been attributed to maternal modification of foreign fetal antigenic expression. In this respect progeny derived from pre-immunized maternal mouse recipients, have been examined for any modification (reduction) of corresponding theta antigen determinants. Two major groups of mice were examined. The first group of embryo transplantation derived AKR homozygotes born from pre-immunized CBA recipients whilst producing the corresponding anti-thetaAKR antibody activity. The second group of naturally derived reciprocal (CBA x AKR)F1 heterozygotes were also born from pre-immunized maternal recipients. In neither group was theta expression found to be modified and the significance of this finding is discussed in respect of other situations where fetal antigenic expression is known to be altered by maternal influence.", "contents": "Failure to suppress theta antigenic expression in progeny derived from pre-immunized maternal recipients. One possible theory concerning the success of the fetus as an allograft has been attributed to maternal modification of foreign fetal antigenic expression. In this respect progeny derived from pre-immunized maternal mouse recipients, have been examined for any modification (reduction) of corresponding theta antigen determinants. Two major groups of mice were examined. The first group of embryo transplantation derived AKR homozygotes born from pre-immunized CBA recipients whilst producing the corresponding anti-thetaAKR antibody activity. The second group of naturally derived reciprocal (CBA x AKR)F1 heterozygotes were also born from pre-immunized maternal recipients. In neither group was theta expression found to be modified and the significance of this finding is discussed in respect of other situations where fetal antigenic expression is known to be altered by maternal influence."}
{"id": "PMID:1087640", "title": "B and T lymphocytes in man. II. Circulating B and T lymphocytes in cancer patients.", "content": "B, T and \"Null\" lymphocytes from 87 coded peripheral blood samples from 33 normals and 54 nonoperable or progressive cancer patients were determined. The controls have higher mean numbers of circulating lymphocytes than the cancer patients. Radiation-treated cancer patients have lower values than non-irradiated patients. This phenomenon is also seen in the relative and absolute T, but not the B lymphocytes. There was no difference in the B lymphocytes among the different groups of patients. However, the controls have the highest and the radiation-treated cancer patients have the lowest absolute numbers of B lymphocytes. Although cancer patients have higher relative numbers of \"Null\" cells than the controls, only the difference between the controls and the radiation-treated cancer patients was statistically significant.", "contents": "B and T lymphocytes in man. II. Circulating B and T lymphocytes in cancer patients. B, T and \"Null\" lymphocytes from 87 coded peripheral blood samples from 33 normals and 54 nonoperable or progressive cancer patients were determined. The controls have higher mean numbers of circulating lymphocytes than the cancer patients. Radiation-treated cancer patients have lower values than non-irradiated patients. This phenomenon is also seen in the relative and absolute T, but not the B lymphocytes. There was no difference in the B lymphocytes among the different groups of patients. However, the controls have the highest and the radiation-treated cancer patients have the lowest absolute numbers of B lymphocytes. Although cancer patients have higher relative numbers of \"Null\" cells than the controls, only the difference between the controls and the radiation-treated cancer patients was statistically significant."}
{"id": "PMID:1087641", "title": "Effects of tetracaine on displacement currents and contraction of frog skeletal muscle.", "content": "The kinetics of mechanical activation of intact fibres were examined with a voltage-clamp technique. Tetracaine (2 mM) increases fifteen- to seventyfold the time required to produce a just visible contraction by cell membrane depolarization. 2. Displacement currents thought to be related to contractile activation remain in 2 mM tetracaine. Their characteristics are virtually identical to those found in the absence of the drug. Displacement currents also remain in fibres immobilized by treatment with 10 mM formaldehyde. 3. Despite its effect on contraction of intact fibres, tetracaine does not diminish contraction tension when Ca is applied directly to the contractile proteins of 'skinned' muscle fibres. The sensitivity of the myofilaments to Ca2+ also remains undiminished. 4. When acting on intact fibres the drug must therefore inhibit Ca2+-release from the sarcoplasmic reticulum. It is estimated that 2 mM tetracaine diminishes more than tenfold the capacity for Ca2+-release in response to cell membrane depolarization.5. If muscle displacement currents represent events linking depolarization to Ca2+-release, then tetracaine must be able to block the release without affecting the potential-sensing portion of the release regulating mechanism. 6. Further experiments on skinned fibres show that tetracaine blocks or greatly diminishes caffeine contractions, but that Cl-induced contractions of normal amplitude are still possible.", "contents": "Effects of tetracaine on displacement currents and contraction of frog skeletal muscle. The kinetics of mechanical activation of intact fibres were examined with a voltage-clamp technique. Tetracaine (2 mM) increases fifteen- to seventyfold the time required to produce a just visible contraction by cell membrane depolarization. 2. Displacement currents thought to be related to contractile activation remain in 2 mM tetracaine. Their characteristics are virtually identical to those found in the absence of the drug. Displacement currents also remain in fibres immobilized by treatment with 10 mM formaldehyde. 3. Despite its effect on contraction of intact fibres, tetracaine does not diminish contraction tension when Ca is applied directly to the contractile proteins of 'skinned' muscle fibres. The sensitivity of the myofilaments to Ca2+ also remains undiminished. 4. When acting on intact fibres the drug must therefore inhibit Ca2+-release from the sarcoplasmic reticulum. It is estimated that 2 mM tetracaine diminishes more than tenfold the capacity for Ca2+-release in response to cell membrane depolarization.5. If muscle displacement currents represent events linking depolarization to Ca2+-release, then tetracaine must be able to block the release without affecting the potential-sensing portion of the release regulating mechanism. 6. Further experiments on skinned fibres show that tetracaine blocks or greatly diminishes caffeine contractions, but that Cl-induced contractions of normal amplitude are still possible."}
{"id": "PMID:1087642", "title": "Differential effects of tetracaine on delayed potassium channels and displacement currents in frog skeletal muscle.", "content": "1. Delayed K+-currents and displacement currents were studied with a voltage-clamp technique. 2. In normal fibres, the conductance of the delayed channel grows e-fold per 3 millivolts at sufficiently negative potentials and reaches a limiting value of 2-10 m-mho/cm2 (mean 5-8 m-mho/cm2) at positive potentials. Adding tetracaine (2 mM) reduces the limiting conductance, shifts the voltage-dependence of the delayed channel to +25 mV more positive potentials and slows the kinetics fourfold. 3. By contrast, the displacement currents are virtually unaltered by 2 mM tetracaine. Their voltage-dependence is shifted by less than 5 mV and their kinetics are unaffected. 4. Tetraethylammonium ions (TEA) are known to slow the kinetics of delayed K+-channels fivefold but fail, like tetracaine, to change the kinetics of the displacement currents. 5. Both tetracaine and TEA have thus large effects on the 'gating' of the delayed channel, yet little or none on the displacement currents. This suggests that the displacement currents in skeletal muscle are for the most part unrelated to the opening and closing of delayed channels. It is estimated that 'gating' the delayed channel in muscle may require no more than 1 or 2% of the observed charge displacement.", "contents": "Differential effects of tetracaine on delayed potassium channels and displacement currents in frog skeletal muscle. 1. Delayed K+-currents and displacement currents were studied with a voltage-clamp technique. 2. In normal fibres, the conductance of the delayed channel grows e-fold per 3 millivolts at sufficiently negative potentials and reaches a limiting value of 2-10 m-mho/cm2 (mean 5-8 m-mho/cm2) at positive potentials. Adding tetracaine (2 mM) reduces the limiting conductance, shifts the voltage-dependence of the delayed channel to +25 mV more positive potentials and slows the kinetics fourfold. 3. By contrast, the displacement currents are virtually unaltered by 2 mM tetracaine. Their voltage-dependence is shifted by less than 5 mV and their kinetics are unaffected. 4. Tetraethylammonium ions (TEA) are known to slow the kinetics of delayed K+-channels fivefold but fail, like tetracaine, to change the kinetics of the displacement currents. 5. Both tetracaine and TEA have thus large effects on the 'gating' of the delayed channel, yet little or none on the displacement currents. This suggests that the displacement currents in skeletal muscle are for the most part unrelated to the opening and closing of delayed channels. It is estimated that 'gating' the delayed channel in muscle may require no more than 1 or 2% of the observed charge displacement."}
{"id": "PMID:1087643", "title": "Measurement of the conductance of the sodium channel from current fluctuations at the node of Ranvier.", "content": "Single myelinated nerve fibres of Rana esculenta were investigated under voltage clamp conditions at 13 degrees C. Fluctuations of steady-state membrane current were measured during the last 152 msec of 190-225 msec pulses depolarizing the membrane by 8-48 mV. Noise power spectral densities were calculated in the frequency range of 6-6-6757 Hz. 2. External application of 150 nM tetrodotoxin (TTX) and/or 10 mM tetraethylammonium (TEA) ion reduced the current fluctuations. The difference of current noise spectra measured in the presence and absence of TTX (TEA) was not changed by the presence of TEA (TTX) during both measurements, and was taken as the spectrum of the Na (K) current fluctuations. 3. Residual current noise during application of both TTX and TEA was, except for some excess noise at the low and high frequency ends of the spectrum, similar to the noise measured from a passive nerve model and could be understood in terms of Nyquist noise of the known resistances and the amplifier noise. 4. Na current fluctuation spectra were interpreted as the sum N/f+SNa(f) where SNa(F) represents the spectrum expected for a set of equal, independent Na channels with only two conductance states (open or closed) which follow Hodgkin-Huxley kinetics. With values of hinfinity, tauh and minfinity measured from macroscopic Na currents, the measured spectra were fitted well by optimizing N, SNa(0) and taum. Values of taum obtained by this method were in fair agreement with values found from macroscopic currents. 5. The 1/f component of Na current noise was roughly proportional to the square of the steady-state Na current, I2. The mean value of N/I2 was (1-1 +/- 0-3) X 10(-4). 6. The current carried by a single Na channel was calculated from fitted spectra and steady-state Na currents measured simultaneously with the current fluctuations. The single channel conductance gamma normalized to zero absolute membrane potential was calculated. The average gamma from twelve measurements at depolarizations of 8-40 mV was 7-9 +/- 0-9 pS (S.E. of mean). The apparent value of gamma was smallest with small depolarizations. Variations of the assumed kinetic properties of the model did not drastically affect the single channel conductance. 7. External application of 0-1 mM-Ni ion lengthened taum in the macroscopic currents and in the fluctuation spectra and enhanced both the steady-state Na current and the current fluctuations. In Ni-treated nodes gamma was smaller than in normal nodes.", "contents": "Measurement of the conductance of the sodium channel from current fluctuations at the node of Ranvier. Single myelinated nerve fibres of Rana esculenta were investigated under voltage clamp conditions at 13 degrees C. Fluctuations of steady-state membrane current were measured during the last 152 msec of 190-225 msec pulses depolarizing the membrane by 8-48 mV. Noise power spectral densities were calculated in the frequency range of 6-6-6757 Hz. 2. External application of 150 nM tetrodotoxin (TTX) and/or 10 mM tetraethylammonium (TEA) ion reduced the current fluctuations. The difference of current noise spectra measured in the presence and absence of TTX (TEA) was not changed by the presence of TEA (TTX) during both measurements, and was taken as the spectrum of the Na (K) current fluctuations. 3. Residual current noise during application of both TTX and TEA was, except for some excess noise at the low and high frequency ends of the spectrum, similar to the noise measured from a passive nerve model and could be understood in terms of Nyquist noise of the known resistances and the amplifier noise. 4. Na current fluctuation spectra were interpreted as the sum N/f+SNa(f) where SNa(F) represents the spectrum expected for a set of equal, independent Na channels with only two conductance states (open or closed) which follow Hodgkin-Huxley kinetics. With values of hinfinity, tauh and minfinity measured from macroscopic Na currents, the measured spectra were fitted well by optimizing N, SNa(0) and taum. Values of taum obtained by this method were in fair agreement with values found from macroscopic currents. 5. The 1/f component of Na current noise was roughly proportional to the square of the steady-state Na current, I2. The mean value of N/I2 was (1-1 +/- 0-3) X 10(-4). 6. The current carried by a single Na channel was calculated from fitted spectra and steady-state Na currents measured simultaneously with the current fluctuations. The single channel conductance gamma normalized to zero absolute membrane potential was calculated. The average gamma from twelve measurements at depolarizations of 8-40 mV was 7-9 +/- 0-9 pS (S.E. of mean). The apparent value of gamma was smallest with small depolarizations. Variations of the assumed kinetic properties of the model did not drastically affect the single channel conductance. 7. External application of 0-1 mM-Ni ion lengthened taum in the macroscopic currents and in the fluctuation spectra and enhanced both the steady-state Na current and the current fluctuations. In Ni-treated nodes gamma was smaller than in normal nodes."}
{"id": "PMID:1087644", "title": "Conductance of the sodium channel in myelinated nerve fibres with modified sodium inactivation.", "content": "1. Na current fluctuations in nodes of Ranvier were measured under voltage clamp conditions as described in the preceding paper (Conti, Hille, Neumcke, Nonner & St\u00e4mpfli, 1976) and analysed in terms of power spectral density calculated for frequencies between 30 Hz and 5 kHz. 2. External (10(-5) g/ml.) Leiurus scorpion venom or Anemonia Toxin II (3 X 10(-5) g/ml.) or internal 20 mM iodate were applied in order to remove or slow down inactivation in part of the Na channels. The treatment increased the steady-state Na current during the noise measurement one-to eight fold over that in normal fibres. 3. Noise spectra were interpreted as the sum of 1/f noise and noise SNa(f) due to all-or-none, open-close transitions of single Na channels. The drug effects on the inactivation could be accounted for either by assuming two populations of channels, one with and one without inactivation, or by postulating a single population with modified inactivation characteristics. 4. Except for an increase in amplitude, the fluctuation spectra SNa(f) were similar to the ones in normal nodes. Again, the time constants taum obtained from the fit of the spectra agreed within a factor of 2 with the values of taum found in the macroscopic Na currents. 5. From the fluctuation spectra, single Na channel conductances gamma of 5-4 +/- 0-4 pS (iodate), 6-7 +/- 0-5 pS (Leiurus) and 7-0 +/- 0-6 pS (Anemonia) were calculated. The value of gamma was not significantly voltage dependent. 6. Our observations indicate that inactivation of Na channels can be modified with at most small effects on the microscopic properties of the activation process and on the conductance of the open channel. They suggest that the h mechanism normally produces all-or-none, open-close changes of conductance.", "contents": "Conductance of the sodium channel in myelinated nerve fibres with modified sodium inactivation. 1. Na current fluctuations in nodes of Ranvier were measured under voltage clamp conditions as described in the preceding paper (Conti, Hille, Neumcke, Nonner & St\u00e4mpfli, 1976) and analysed in terms of power spectral density calculated for frequencies between 30 Hz and 5 kHz. 2. External (10(-5) g/ml.) Leiurus scorpion venom or Anemonia Toxin II (3 X 10(-5) g/ml.) or internal 20 mM iodate were applied in order to remove or slow down inactivation in part of the Na channels. The treatment increased the steady-state Na current during the noise measurement one-to eight fold over that in normal fibres. 3. Noise spectra were interpreted as the sum of 1/f noise and noise SNa(f) due to all-or-none, open-close transitions of single Na channels. The drug effects on the inactivation could be accounted for either by assuming two populations of channels, one with and one without inactivation, or by postulating a single population with modified inactivation characteristics. 4. Except for an increase in amplitude, the fluctuation spectra SNa(f) were similar to the ones in normal nodes. Again, the time constants taum obtained from the fit of the spectra agreed within a factor of 2 with the values of taum found in the macroscopic Na currents. 5. From the fluctuation spectra, single Na channel conductances gamma of 5-4 +/- 0-4 pS (iodate), 6-7 +/- 0-5 pS (Leiurus) and 7-0 +/- 0-6 pS (Anemonia) were calculated. The value of gamma was not significantly voltage dependent. 6. Our observations indicate that inactivation of Na channels can be modified with at most small effects on the microscopic properties of the activation process and on the conductance of the open channel. They suggest that the h mechanism normally produces all-or-none, open-close changes of conductance."}
{"id": "PMID:1087648", "title": "Excitability and functional organization of cutaneous tactile units of the bullfrog (R. catesbeiana).", "content": "Frog skin touch receptors are discrete structures which appear as dome-shaped translucent elevations of the epidermis. These domes are particularly responsive to direct touch. Tactile stimulation of adjacent skin never caused impulse activity, except when the skin distortion disturbed the domes. On stimulation at threshold intensity, the response of all units studied always consisted of a single impulse whether the stimulus energy was electrical or mechanical. While most tactile units discharged one impulse upon application of mechanical stimulation, a few also discharged one impulse upon stimulus release (on-off response). No after discharge was seen at stimulus strengths of three or four times threshold or after repetitive stimulation at 1,000 pps. The range of distribution of active domes/receptive field was 1--14. Generally the most sensitive domes appeared to be in or near center field. Twice as many of the tactile units responded more readily to cold than to warmth. The impulse frequency of units responding to thermal stimulation ranged from 3-11/sec. The conduction velocity of tactile units measured was within a range of 9--25 m/sec, with a mean of 12 m/sec. The results suggest that the following: 1) the existence of discrete structures which respond to tactile stimuli; a phylogenetic relationship might be postulated; 2) cold/touch receptors which appear to be present in greater quantity in the frog's skin than are warm/touch receptors, 3) touch units which should be considered primarily as mechanoreceptors with a possible secondary function as thermoreceptors.", "contents": "Excitability and functional organization of cutaneous tactile units of the bullfrog (R. catesbeiana). Frog skin touch receptors are discrete structures which appear as dome-shaped translucent elevations of the epidermis. These domes are particularly responsive to direct touch. Tactile stimulation of adjacent skin never caused impulse activity, except when the skin distortion disturbed the domes. On stimulation at threshold intensity, the response of all units studied always consisted of a single impulse whether the stimulus energy was electrical or mechanical. While most tactile units discharged one impulse upon application of mechanical stimulation, a few also discharged one impulse upon stimulus release (on-off response). No after discharge was seen at stimulus strengths of three or four times threshold or after repetitive stimulation at 1,000 pps. The range of distribution of active domes/receptive field was 1--14. Generally the most sensitive domes appeared to be in or near center field. Twice as many of the tactile units responded more readily to cold than to warmth. The impulse frequency of units responding to thermal stimulation ranged from 3-11/sec. The conduction velocity of tactile units measured was within a range of 9--25 m/sec, with a mean of 12 m/sec. The results suggest that the following: 1) the existence of discrete structures which respond to tactile stimuli; a phylogenetic relationship might be postulated; 2) cold/touch receptors which appear to be present in greater quantity in the frog's skin than are warm/touch receptors, 3) touch units which should be considered primarily as mechanoreceptors with a possible secondary function as thermoreceptors."}
{"id": "PMID:1087650", "title": "Studies on recovery mechanism from rinderpest virus infection in rabbits. I. Effect of anti-thymocyte serum and thymectomy.", "content": "The role of cell-mediated immunity in recovery from rinderpest virus infection in rabbits was investigated by application of immunosuppressive procedures, i.e., treatment with anti-thymocyte serum and combined treatment with thymectomy and anti-thymocyte serum, both of which were confirmed to depress significantly cell-mediated immunity in rabbits. The immunosuppressed animals recovered in almost the normal fashion in terms of clinical signs, of virus clearance from the blood and lymphoid tissues and of repair of the lesions. It was suggested that the thymus-dependent cell-mediated immunity may not be essential in recovery from rinderpest virus infection. Possibility of participation of other recovery mechanisms was discussed.", "contents": "Studies on recovery mechanism from rinderpest virus infection in rabbits. I. Effect of anti-thymocyte serum and thymectomy. The role of cell-mediated immunity in recovery from rinderpest virus infection in rabbits was investigated by application of immunosuppressive procedures, i.e., treatment with anti-thymocyte serum and combined treatment with thymectomy and anti-thymocyte serum, both of which were confirmed to depress significantly cell-mediated immunity in rabbits. The immunosuppressed animals recovered in almost the normal fashion in terms of clinical signs, of virus clearance from the blood and lymphoid tissues and of repair of the lesions. It was suggested that the thymus-dependent cell-mediated immunity may not be essential in recovery from rinderpest virus infection. Possibility of participation of other recovery mechanisms was discussed."}
{"id": "PMID:1087672", "title": "The receptive mechanism of various metallic ions in the lateral-line organ of the tadpoles of Rana catesbeiana.", "content": "The stimulating effect on the receptor organ of various mono-and divalent metallic ions on the lateral-line nerve of tadpoles was studied. The orders of the effectiveness were Ag + greater than T1+ greater than K+==Na+ greater than Li for monovalent ions and Sr2+ greater than Mg2+ greater than or equal too Ba2+ greater than Cd2+ greater than or equal too Co2+ greater than Mn2+ greater than Zn2+ for divalent ions. Both agree well with the order of Pearson's softness parameter for monovalent ions and Edwards' modified parameter for divalent ones. All sorts of divalent cations exhibited suppressive effects on the stimulating effect of the monovalent cations when they were applied together with the monovalent cations. A simple selection rule was found for the suppressive effect. The stimulating effect of Na+ or K+ was suppressed by all sorts of divalent cations. But the effect of Ag+ was suppressed by Cd2+, but not by Mg2+ nor by Ca2+. In order to remove the Ag+ effect, a dilute solution of DTT was used, although the effect of K+ or Na+ was removed easily by rinsing with distilled water. From these results the receptive mechanism of the endorgan was thought to be chemical adsorption of ions in the receptor cell membrane. Based on the principle of \"hard and soft acid and base\", it is suggested that there are two types of binding sites for the ions on the membrane, a soft site and a hard one.", "contents": "The receptive mechanism of various metallic ions in the lateral-line organ of the tadpoles of Rana catesbeiana. The stimulating effect on the receptor organ of various mono-and divalent metallic ions on the lateral-line nerve of tadpoles was studied. The orders of the effectiveness were Ag + greater than T1+ greater than K+==Na+ greater than Li for monovalent ions and Sr2+ greater than Mg2+ greater than or equal too Ba2+ greater than Cd2+ greater than or equal too Co2+ greater than Mn2+ greater than Zn2+ for divalent ions. Both agree well with the order of Pearson's softness parameter for monovalent ions and Edwards' modified parameter for divalent ones. All sorts of divalent cations exhibited suppressive effects on the stimulating effect of the monovalent cations when they were applied together with the monovalent cations. A simple selection rule was found for the suppressive effect. The stimulating effect of Na+ or K+ was suppressed by all sorts of divalent cations. But the effect of Ag+ was suppressed by Cd2+, but not by Mg2+ nor by Ca2+. In order to remove the Ag+ effect, a dilute solution of DTT was used, although the effect of K+ or Na+ was removed easily by rinsing with distilled water. From these results the receptive mechanism of the endorgan was thought to be chemical adsorption of ions in the receptor cell membrane. Based on the principle of \"hard and soft acid and base\", it is suggested that there are two types of binding sites for the ions on the membrane, a soft site and a hard one."}
{"id": "PMID:1087674", "title": "Rheumatoid disease without arthritis.", "content": "A middle-aged man developed multiple subcutaneous rheumatoid granulomata, high titer of rheumatoid factor, diffuse interstitial pulmonary fibrosis and digital clubbing in the absence of clinicoradiological evidence of synovial disease. This patient supports the concept of rheumatoid disease without arthritis.", "contents": "Rheumatoid disease without arthritis. A middle-aged man developed multiple subcutaneous rheumatoid granulomata, high titer of rheumatoid factor, diffuse interstitial pulmonary fibrosis and digital clubbing in the absence of clinicoradiological evidence of synovial disease. This patient supports the concept of rheumatoid disease without arthritis."}
{"id": "PMID:1087702", "title": "Effect of (+) amphetamine on monoamine synthesis and metabolism after axotomy in rat forebrain.", "content": "(+)Amphetamine is known to produce feedback inhibition of catecholamine-containing neurons in the central nervous system due to its indirect receptor stimulating properties. This feedback control may involve postsynaptic receptors and neuronal loops or presynaptic receptors and may be restricted to the catecholaminergic terminal system. In order to study the effect of (+)amphetamine on catecholamine synthesis and metabolism in the terminal system changes in impulse flow were eliminated by cutting the ascending monaminergic axons. Axotomy of the ascending monominergic fibers by means of a complete transverse cerebral hemisection resulted in a 3-fold increase in Dopa formation in the lesioned forebrain during 30 min after inhibition of the aromatic amino acid decarboxylase with 3-hydroxybenzylhydrazine HCl, 100 mg/kg i.p. (+)-Amphetamine sulfate, 10 mg/kg i.p. antagonized the hemisection-induced increase in Dopa formation and reduced the formation of 5-hydroxytryptophan. Pretreatment with haloperidol, 5 mg/kg i.p. failed to counteract the effect of (+)amphetamine. In the intact forebrain the stimulation of Dopa accumulation was more than additive after combined treatment with haloperidol and (+)amphetamine. Hemitransection retarded the disappearance of dopamine and noradrenaline after administration of alpha-methyl-p-tyrosine methylester HCl,300 mg/kg. (+)-Amphetamine, 10 mg/kg accelerated the utilization of dopamine on the lesioned side. Hemitransection reduced the formation of 3-methoxytyramine during 1 h after pargyline, 75 mg/kg i.p. After (+)amphetamine 3-methoxytyramine formation in the intact forebrain was 3 times higher than in the lesioned forebrain. The action of (+)amphetamine on dopamine synthesis and release appears to be dependent on the firing rate in dopamine neurons.", "contents": "Effect of (+) amphetamine on monoamine synthesis and metabolism after axotomy in rat forebrain. (+)Amphetamine is known to produce feedback inhibition of catecholamine-containing neurons in the central nervous system due to its indirect receptor stimulating properties. This feedback control may involve postsynaptic receptors and neuronal loops or presynaptic receptors and may be restricted to the catecholaminergic terminal system. In order to study the effect of (+)amphetamine on catecholamine synthesis and metabolism in the terminal system changes in impulse flow were eliminated by cutting the ascending monaminergic axons. Axotomy of the ascending monominergic fibers by means of a complete transverse cerebral hemisection resulted in a 3-fold increase in Dopa formation in the lesioned forebrain during 30 min after inhibition of the aromatic amino acid decarboxylase with 3-hydroxybenzylhydrazine HCl, 100 mg/kg i.p. (+)-Amphetamine sulfate, 10 mg/kg i.p. antagonized the hemisection-induced increase in Dopa formation and reduced the formation of 5-hydroxytryptophan. Pretreatment with haloperidol, 5 mg/kg i.p. failed to counteract the effect of (+)amphetamine. In the intact forebrain the stimulation of Dopa accumulation was more than additive after combined treatment with haloperidol and (+)amphetamine. Hemitransection retarded the disappearance of dopamine and noradrenaline after administration of alpha-methyl-p-tyrosine methylester HCl,300 mg/kg. (+)-Amphetamine, 10 mg/kg accelerated the utilization of dopamine on the lesioned side. Hemitransection reduced the formation of 3-methoxytyramine during 1 h after pargyline, 75 mg/kg i.p. After (+)amphetamine 3-methoxytyramine formation in the intact forebrain was 3 times higher than in the lesioned forebrain. The action of (+)amphetamine on dopamine synthesis and release appears to be dependent on the firing rate in dopamine neurons."}
{"id": "PMID:1087706", "title": "[Dimer-X in the intracranial subarachnoid space--its toxicity (author's transl)].", "content": "The reaction of the rats to injection of Dimer-X into the intracranial subarachnoid space or the cerebrum was examined. When 28% iodine Dimer-X was injected into the subarachnoid space, 7 of 10 rats showed agitation, which is the most severe complication, and all died within one hour. All of the reactions were reduced with the lower concentrations of Dimer-X. The rats which were premedicated with Valium and/or Decadron revealed fewer and less severe complications. It is believed that the intracerebral injection method has many disadvantages, the results of its experiment are therefore reported without further elaboration. From the results of the experiment it is concluded as follows: 1) Dimer-X should not be injected into the intracranial subarachnoid space. 2) Dimer-X of low concentration in the subarachnoid space reduces the complications. 3) Complications from the subarachnoid application of Dimer-X can be reduced by premedication with Valium and Decadron.", "contents": "[Dimer-X in the intracranial subarachnoid space--its toxicity (author's transl)]. The reaction of the rats to injection of Dimer-X into the intracranial subarachnoid space or the cerebrum was examined. When 28% iodine Dimer-X was injected into the subarachnoid space, 7 of 10 rats showed agitation, which is the most severe complication, and all died within one hour. All of the reactions were reduced with the lower concentrations of Dimer-X. The rats which were premedicated with Valium and/or Decadron revealed fewer and less severe complications. It is believed that the intracerebral injection method has many disadvantages, the results of its experiment are therefore reported without further elaboration. From the results of the experiment it is concluded as follows: 1) Dimer-X should not be injected into the intracranial subarachnoid space. 2) Dimer-X of low concentration in the subarachnoid space reduces the complications. 3) Complications from the subarachnoid application of Dimer-X can be reduced by premedication with Valium and Decadron."}
{"id": "PMID:1087712", "title": "Transmission of jaagsiekte (ovine pulmonary adenomatosis) by means of a permanent epithelial cell line established from affected lungs.", "content": "An epithelial cell line, designated JS-15,4, has been established in culture from jaagiekte lesions and subcultured in vitro for almost 2 years. It exhibits morphological and other features of transformed cells and has been shown by electron microscopy to consist of type B ovine alveolar epithelial cells. Jaagiekte was successfully transmitted to 3 new-born lambs by the intratracheal injection of cells following immunosuppressive treatment with either anti-thymocyte immunoglobulin alone or combined with anti-macrophage immunoglobulin. Incubation periods as short as 10 weeks were recorded. Evidence was also obtained that natural transmission may result from the inhalation of viable cells.", "contents": "Transmission of jaagsiekte (ovine pulmonary adenomatosis) by means of a permanent epithelial cell line established from affected lungs. An epithelial cell line, designated JS-15,4, has been established in culture from jaagiekte lesions and subcultured in vitro for almost 2 years. It exhibits morphological and other features of transformed cells and has been shown by electron microscopy to consist of type B ovine alveolar epithelial cells. Jaagiekte was successfully transmitted to 3 new-born lambs by the intratracheal injection of cells following immunosuppressive treatment with either anti-thymocyte immunoglobulin alone or combined with anti-macrophage immunoglobulin. Incubation periods as short as 10 weeks were recorded. Evidence was also obtained that natural transmission may result from the inhalation of viable cells."}
{"id": "PMID:1087713", "title": "Fossa posterior measurements: significance of sella to floor of 4th ventricle measurements (normal position of floor of 4th ventricle).", "content": "Two proportional methods are introduced to determine the normal position of the floor of the 4th ventricle expressed by two preventricular ratios: (see article); where ds = dorsum sellae, Ts = tuberculum sellae and Tw = Twining's line. T4 is the intersection between Tw and the floor of the 4th ventricle. The methods give information of the position of the 4th ventricle, the diameter of pons and the a-p diameter of the pituitary fossa.", "contents": "Fossa posterior measurements: significance of sella to floor of 4th ventricle measurements (normal position of floor of 4th ventricle). Two proportional methods are introduced to determine the normal position of the floor of the 4th ventricle expressed by two preventricular ratios: (see article); where ds = dorsum sellae, Ts = tuberculum sellae and Tw = Twining's line. T4 is the intersection between Tw and the floor of the 4th ventricle. The methods give information of the position of the 4th ventricle, the diameter of pons and the a-p diameter of the pituitary fossa."}
{"id": "PMID:1087720", "title": "Fetal hemopoiesis in diffusion chamber cultures: II. Cell proliferation and differentiation.", "content": "The growth and differenciation of hemopoietic cells from murine fetal liver (FL) and adult bone marrow (ABM) in diffusion chambers (DC) implanted into normal CF1 mice were evaluated. Initially FL suspensions were approximately 90% erythrocytic, but after 4 days in DC implanted into either normal or phenylhydrazine (anemic) pretreated hosts, growth was essentially restricted to the granulocyte-macrophage cell types. The number of in vitro colony forming cells (CFC), as assayed in a double layer soft-agar technique was determined after varying periods of growth in DC of both ABM and FL. Both groups showed a progressive decline in CFC number. FL and ABM CFC generated the same proportion of different morphologic types of soft agar colonies. After 7 days of DC culture, there was a decrease in the proportion of macrophage colonies from both groupes. During DC culture the ratio of clusters (3-50 cells to colonies progressively increased in both groups suggesting a parent-progeny relationship between CFC and cluster forming cells. The results of these studies provide further evidence that the environment rather than properties intrinsic to murine stem cell determines the pattern of proliferation.", "contents": "Fetal hemopoiesis in diffusion chamber cultures: II. Cell proliferation and differentiation. The growth and differenciation of hemopoietic cells from murine fetal liver (FL) and adult bone marrow (ABM) in diffusion chambers (DC) implanted into normal CF1 mice were evaluated. Initially FL suspensions were approximately 90% erythrocytic, but after 4 days in DC implanted into either normal or phenylhydrazine (anemic) pretreated hosts, growth was essentially restricted to the granulocyte-macrophage cell types. The number of in vitro colony forming cells (CFC), as assayed in a double layer soft-agar technique was determined after varying periods of growth in DC of both ABM and FL. Both groups showed a progressive decline in CFC number. FL and ABM CFC generated the same proportion of different morphologic types of soft agar colonies. After 7 days of DC culture, there was a decrease in the proportion of macrophage colonies from both groupes. During DC culture the ratio of clusters (3-50 cells to colonies progressively increased in both groups suggesting a parent-progeny relationship between CFC and cluster forming cells. The results of these studies provide further evidence that the environment rather than properties intrinsic to murine stem cell determines the pattern of proliferation."}
{"id": "PMID:1087721", "title": "[Clinical and biological studies in the Bernard-Soulier syndrome (author's transl)].", "content": "Bernard-Soulier syndrome is a rare constitutional thrombopathy, the main clinical feature is a bleeding tendency which is variable. The long bleeding time, in spite of a normal or little decreased platelet count, has been recently explained: the giant platelets have a defective adhesion to subendothelium. This abnormal adhesion could be related with an abnormal pattern of platelet membrane glycoproteins. Interactions between vessel wall, von Willebrand's factor and platelets are discussed.", "contents": "[Clinical and biological studies in the Bernard-Soulier syndrome (author's transl)]. Bernard-Soulier syndrome is a rare constitutional thrombopathy, the main clinical feature is a bleeding tendency which is variable. The long bleeding time, in spite of a normal or little decreased platelet count, has been recently explained: the giant platelets have a defective adhesion to subendothelium. This abnormal adhesion could be related with an abnormal pattern of platelet membrane glycoproteins. Interactions between vessel wall, von Willebrand's factor and platelets are discussed."}
{"id": "PMID:1087722", "title": "Effect of long-term hyperglucocorticoidism on the neuronal morphology of the trigeminal ganglion of the mouse.", "content": "Immature mice were treated for up to 12 weeks with daily doses of triamcinolone diacetate. The trigeminal ganglion was studied histologically at regular intervals. By the tenth injection significant morphological changes were noted in the various nerve cells, followed by marked cellular deterioration in the form of lysis or pyknosis. A possible explanation for the above findings is discussed.", "contents": "Effect of long-term hyperglucocorticoidism on the neuronal morphology of the trigeminal ganglion of the mouse. Immature mice were treated for up to 12 weeks with daily doses of triamcinolone diacetate. The trigeminal ganglion was studied histologically at regular intervals. By the tenth injection significant morphological changes were noted in the various nerve cells, followed by marked cellular deterioration in the form of lysis or pyknosis. A possible explanation for the above findings is discussed."}
{"id": "PMID:1087724", "title": "Technical possibilities for coronary artery bypass surgery and the outlook for graft patency.", "content": "In an attempt to analyse the results of saphenous bypass grafting 213 patients were re-investigated by repeat coronary, graft and left ventricular angiography. In this series, the presence of diffuse arterial disease was not considered as a contra-indication to operation. Instead, additional endarterectomy was performed. The overall patency rate was 88% and was not significantly altered by the passage of time, although the patency rate during the first 6 months was 94%. The patency rate did not appear to be influenced by the age of the patient, the severity of the lesion pre-operatively, the artery grafted nor additional endarterectomy. The run-off was judged to be good in 82% of grafts investigated before 6 months, compared to 70% of those investigated later. Progress of the disease in the native vessels appeared to be slightly accelerated in the segment proximal to the graft.", "contents": "Technical possibilities for coronary artery bypass surgery and the outlook for graft patency. In an attempt to analyse the results of saphenous bypass grafting 213 patients were re-investigated by repeat coronary, graft and left ventricular angiography. In this series, the presence of diffuse arterial disease was not considered as a contra-indication to operation. Instead, additional endarterectomy was performed. The overall patency rate was 88% and was not significantly altered by the passage of time, although the patency rate during the first 6 months was 94%. The patency rate did not appear to be influenced by the age of the patient, the severity of the lesion pre-operatively, the artery grafted nor additional endarterectomy. The run-off was judged to be good in 82% of grafts investigated before 6 months, compared to 70% of those investigated later. Progress of the disease in the native vessels appeared to be slightly accelerated in the segment proximal to the graft."}
{"id": "PMID:1087725", "title": "Emergency myocardial revascularization.", "content": "From 1969 to 1975, 175 patients with acute coronary insufficiency underwent emergency saphenous vein aorto-coronary bypass grafting (SVBG). The patients were divided into two groups: group I, unstable angina (165 patients) and group II, acute evolving myocardial infarction (ten patients). In group I, the hospital mortality was 8-4%, the incidence of post-operative myocardial infarction was 10-3%. Long-term follow-up was obtained for an average of 25 months, functional improvement was definite in the majority of the patients and actuarial survival curves show 87% patients alive at the end of 48 months. In group II, the hospital mortality was 30%; seven of ten patients had good results.", "contents": "Emergency myocardial revascularization. From 1969 to 1975, 175 patients with acute coronary insufficiency underwent emergency saphenous vein aorto-coronary bypass grafting (SVBG). The patients were divided into two groups: group I, unstable angina (165 patients) and group II, acute evolving myocardial infarction (ten patients). In group I, the hospital mortality was 8-4%, the incidence of post-operative myocardial infarction was 10-3%. Long-term follow-up was obtained for an average of 25 months, functional improvement was definite in the majority of the patients and actuarial survival curves show 87% patients alive at the end of 48 months. In group II, the hospital mortality was 30%; seven of ten patients had good results."}
{"id": "PMID:1087726", "title": "Correlation between anti-DNA antibody titre and psychiatric manifestations in systemic lupus erythematosus.", "content": "A patient with systemic lupus erythematosus with predominant psychiatric involvement was followed during two psychotic exacerbations of her disease. A high correlation was found between disease activity and the titre of anti-DNA antibodies. Disease activity was preceded and accompanied by a high titre, while remission was associated with low titre. The significance of this correlation for the pathogenesis, diagnosis and managment of the psychiatric symptoms of systemic lupus erythematosus are discussed.", "contents": "Correlation between anti-DNA antibody titre and psychiatric manifestations in systemic lupus erythematosus. A patient with systemic lupus erythematosus with predominant psychiatric involvement was followed during two psychotic exacerbations of her disease. A high correlation was found between disease activity and the titre of anti-DNA antibodies. Disease activity was preceded and accompanied by a high titre, while remission was associated with low titre. The significance of this correlation for the pathogenesis, diagnosis and managment of the psychiatric symptoms of systemic lupus erythematosus are discussed."}
{"id": "PMID:1087731", "title": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part 1: Implications of reproducible mood elevation.", "content": "L-5-hydroxytryptophan ethylester (Ro 3-5940), a new soluble form of this serotonin precursor, was administered to 26 healthy, non-depressed subjects after premedication with the peripheral decarboxylase inhibitor benserazide (Ro 4-6402) in a total of 51 infusions. Those conditions were chosen for the main trial which in a pre-trial investigation had proved to combine minimal side effects with clear central effects in particular the observed marked mood elevation after 1-5HTP. Using these standardized conditions of application, an interindividually similar pattern of the time course of substance effects could be shown, and convincing evidence was deducible for an objective mood elevating effect of 1-5HTP infusion. Amongst the most impressive results was the parallelity of the time course of mood changes and concomitant changes in serum growth hormone levels. Especially emphasized are the important questions of effectivity, specificity and clinical practicability or safety, which are essential for any precursor study. Arguments are presented supporting the assumption that primarily serotoninergic changes underlie these mood effects. In our opinion this mode of i.v. application of 1-5HTP represents a practicable strategy for investigating biochemical hypotheses as to serotonin mediated normal and deviant human behaviour, especially in affective disorders.", "contents": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part 1: Implications of reproducible mood elevation. L-5-hydroxytryptophan ethylester (Ro 3-5940), a new soluble form of this serotonin precursor, was administered to 26 healthy, non-depressed subjects after premedication with the peripheral decarboxylase inhibitor benserazide (Ro 4-6402) in a total of 51 infusions. Those conditions were chosen for the main trial which in a pre-trial investigation had proved to combine minimal side effects with clear central effects in particular the observed marked mood elevation after 1-5HTP. Using these standardized conditions of application, an interindividually similar pattern of the time course of substance effects could be shown, and convincing evidence was deducible for an objective mood elevating effect of 1-5HTP infusion. Amongst the most impressive results was the parallelity of the time course of mood changes and concomitant changes in serum growth hormone levels. Especially emphasized are the important questions of effectivity, specificity and clinical practicability or safety, which are essential for any precursor study. Arguments are presented supporting the assumption that primarily serotoninergic changes underlie these mood effects. In our opinion this mode of i.v. application of 1-5HTP represents a practicable strategy for investigating biochemical hypotheses as to serotonin mediated normal and deviant human behaviour, especially in affective disorders."}
{"id": "PMID:1087732", "title": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part II: profile of psychotropic effects derived from protocols and psychometric investigations.", "content": "L-5HTP was found to act primarily on mood, and unequivocally in the direction of elation. The subjective, protocols, psychometric results, and a double-blind study demonstrated convincingly and intra-individually consistent course of action of the substance under the chosen conditions of application. This course of effects can be described in three phases: In a first phase (about 0 to 1 1/2 hours after end of infusion) an intensive mood elevation (mostly experienced as a disharmonious feeling), psychomotoric activity, changed perception, as well as somatic side effects were observed. A second phase (about 1 1/2 to 3 1/2 hours after end of infusion) was characterized by elevated mood (feeling of well-being) together with a tendency to inactivity, whereas other phenomena were less often reported. A third phase (about 3 1/2 to 6 hours after end of infusion) was characterized by mood decline and the feeling of having again reached \"normality\". Side effects again tended to occur. It is shown that neither placebo nor situative effects greatly influenced elevation of mood or other substance effects. This is demonstrated by the homogenous time course and the blind studies.", "contents": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part II: profile of psychotropic effects derived from protocols and psychometric investigations. L-5HTP was found to act primarily on mood, and unequivocally in the direction of elation. The subjective, protocols, psychometric results, and a double-blind study demonstrated convincingly and intra-individually consistent course of action of the substance under the chosen conditions of application. This course of effects can be described in three phases: In a first phase (about 0 to 1 1/2 hours after end of infusion) an intensive mood elevation (mostly experienced as a disharmonious feeling), psychomotoric activity, changed perception, as well as somatic side effects were observed. A second phase (about 1 1/2 to 3 1/2 hours after end of infusion) was characterized by elevated mood (feeling of well-being) together with a tendency to inactivity, whereas other phenomena were less often reported. A third phase (about 3 1/2 to 6 hours after end of infusion) was characterized by mood decline and the feeling of having again reached \"normality\". Side effects again tended to occur. It is shown that neither placebo nor situative effects greatly influenced elevation of mood or other substance effects. This is demonstrated by the homogenous time course and the blind studies."}
{"id": "PMID:1087733", "title": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part III: Neuroendocrinological and biochemical changes.", "content": "In a precursor study with i.v. 1-5HTP (Ro 3-5940) after peripheral decarboxylase inhibition with benserazide (Ro 4-4602) in healthy human subjects the following biochemical changes were found: 1. Prolactin release was stimulated by benserazide alone, and further stimulated by 1-5HTP (more in women than in men). 2. Basal growth hormone secretion was not affected by benserazide, but was significantly stimulated by 1-5HTP, often to extremely high values. The time course of growth hormone release followed that of mood elevation in 8/11 subjects (and was even correlated in 3), providing data for speculation as to the interrelationships between affective state and neuroendocrinological function. 3. Cortisol levels were higher than normal throughout the experiment, but followed the diurnal decline. A late stimulation of cortisol release after 1-5HTP was observed. 4. The lack of increased platelet serotonin after 1-5HTP indicated efficacious peripheral decarboxylase inhibition by benserazide. 5. Benserazide treatment inhibited platelet MAO activity in certain subjects. Short-term increases in MAO activity after 1-5HTP were observed. 6. 1-5HTP displaced albumin-bound tryptophan by 20%.", "contents": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part III: Neuroendocrinological and biochemical changes. In a precursor study with i.v. 1-5HTP (Ro 3-5940) after peripheral decarboxylase inhibition with benserazide (Ro 4-4602) in healthy human subjects the following biochemical changes were found: 1. Prolactin release was stimulated by benserazide alone, and further stimulated by 1-5HTP (more in women than in men). 2. Basal growth hormone secretion was not affected by benserazide, but was significantly stimulated by 1-5HTP, often to extremely high values. The time course of growth hormone release followed that of mood elevation in 8/11 subjects (and was even correlated in 3), providing data for speculation as to the interrelationships between affective state and neuroendocrinological function. 3. Cortisol levels were higher than normal throughout the experiment, but followed the diurnal decline. A late stimulation of cortisol release after 1-5HTP was observed. 4. The lack of increased platelet serotonin after 1-5HTP indicated efficacious peripheral decarboxylase inhibition by benserazide. 5. Benserazide treatment inhibited platelet MAO activity in certain subjects. Short-term increases in MAO activity after 1-5HTP were observed. 6. 1-5HTP displaced albumin-bound tryptophan by 20%."}
{"id": "PMID:1087734", "title": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part IV: Effects on body temperature and cardiovascular functions.", "content": "In 14 healthy subjects the effect of a new soluble 1-5HTP ester (Ro 3-5940) in combination with a peripheral decarboxylase inhibitor on circulatory variables and body temperature was studied. The orthostatic adaptative functions were impaired while no consistent changes were observed in resting parameters. With the exception of an initial rise during infusion, measurements of oral temperature in the group as a whole showed no significant changes. However, if gender was considered, a long-lasting fall of temperature in males (N = 7) and rise in females (n = 6) was observed. The greatest difference between the hypothermic reaction in men and the hyperthermic reaction in women occured two hours after the end of infusion, at the same time where mood effects were most intense. The temperature findings support a modulatory function of central serotoninergic mechanisms in thermoregulation in man and emphasize the importance of sex specific factors in pharmacological studies.", "contents": "Intravenous L-5-hydroxytryptophan in normal subjects: an interdisciplinary precursor loading study. Part IV: Effects on body temperature and cardiovascular functions. In 14 healthy subjects the effect of a new soluble 1-5HTP ester (Ro 3-5940) in combination with a peripheral decarboxylase inhibitor on circulatory variables and body temperature was studied. The orthostatic adaptative functions were impaired while no consistent changes were observed in resting parameters. With the exception of an initial rise during infusion, measurements of oral temperature in the group as a whole showed no significant changes. However, if gender was considered, a long-lasting fall of temperature in males (N = 7) and rise in females (n = 6) was observed. The greatest difference between the hypothermic reaction in men and the hyperthermic reaction in women occured two hours after the end of infusion, at the same time where mood effects were most intense. The temperature findings support a modulatory function of central serotoninergic mechanisms in thermoregulation in man and emphasize the importance of sex specific factors in pharmacological studies."}
{"id": "PMID:1087743", "title": "Gastro-intestinal microbleeding under acetylsalicylic acid, ketoprofen and placebo.", "content": "A quantitative comparison of gastro-intestinal microbleeding induced by acetylsalicylic acid (ASA), 3.6 g daily, ketoprofen (KETO), 200 mg daily and placebo (P) was undertaken in 12 normal volunteers using a double-blind factorial design with repeated measures. We conclude that KETO induces less gastro-intestinal bleeding than ASA but more than placebo and that there is a significant residual bleeding under placebo following ASA.", "contents": "Gastro-intestinal microbleeding under acetylsalicylic acid, ketoprofen and placebo. A quantitative comparison of gastro-intestinal microbleeding induced by acetylsalicylic acid (ASA), 3.6 g daily, ketoprofen (KETO), 200 mg daily and placebo (P) was undertaken in 12 normal volunteers using a double-blind factorial design with repeated measures. We conclude that KETO induces less gastro-intestinal bleeding than ASA but more than placebo and that there is a significant residual bleeding under placebo following ASA."}
{"id": "PMID:1087744", "title": "[Possibilities of differential diagnosis for positive ventriculography in children (author's transl)].", "content": "On the basis of 112 positive ventriculographies showing enlargement of the ventrical system in children and adolescents and excluding supratentorial processes, the possibilities and limitations of ventriculographic differential diagnosis were presented. Pathognomonic findings were found in inflammatory aquaduct occlusions and in meningoceles combined with hydrocephaly. In neoplastic or otherwise induced ventricular enlargement, statements can be made only in regard to the localization and often to the extent of the process. In spite of these limitations, the advantages of the positive contrast are obvious.", "contents": "[Possibilities of differential diagnosis for positive ventriculography in children (author's transl)]. On the basis of 112 positive ventriculographies showing enlargement of the ventrical system in children and adolescents and excluding supratentorial processes, the possibilities and limitations of ventriculographic differential diagnosis were presented. Pathognomonic findings were found in inflammatory aquaduct occlusions and in meningoceles combined with hydrocephaly. In neoplastic or otherwise induced ventricular enlargement, statements can be made only in regard to the localization and often to the extent of the process. In spite of these limitations, the advantages of the positive contrast are obvious."}
{"id": "PMID:1087745", "title": "Evidence for the release of mitogenic factors by both T and B lymphocytes in the human.", "content": "Lymphocytes incubated with antigens or nonspecific stimulatory agents may release factors that induce DNA synthesis in other lymphocytes. The aim of this investigation was to determine whether such mitogenic factors (MF) are produced by both T and B cells in the human. Both T- or B-cell-enriched cell preparations from peripheral blood were found to release MF during or after incubation with phytohemagglutinin or pokeweed mitogen. Concanavalin A covalently bound to Sepharose triggered B-cell preparations to higher MF release than either T cells or unfractionated lymphocyte suspensions. These results strongly indicate that both T and B cells in the human are able to produce MF.", "contents": "Evidence for the release of mitogenic factors by both T and B lymphocytes in the human. Lymphocytes incubated with antigens or nonspecific stimulatory agents may release factors that induce DNA synthesis in other lymphocytes. The aim of this investigation was to determine whether such mitogenic factors (MF) are produced by both T and B cells in the human. Both T- or B-cell-enriched cell preparations from peripheral blood were found to release MF during or after incubation with phytohemagglutinin or pokeweed mitogen. Concanavalin A covalently bound to Sepharose triggered B-cell preparations to higher MF release than either T cells or unfractionated lymphocyte suspensions. These results strongly indicate that both T and B cells in the human are able to produce MF."}
{"id": "PMID:1087746", "title": "DNA-synthetic responses of fractionated human lymphocytes exposed to lymphocyte-derived mitogenic factor.", "content": "Human peripheral lymphocytes that have been exposed to phytohemagglutinin (PHA) release mitogenic factors (MF) on subsequent incubation in fresh medium. In this investigation, MF-rich supernatants were analyzed for their ability to induce DNA synthesis in various fractions of peripheral human lymphocytes. MF was found to induce higher DNA synthesis in T-cell preparations that in unfractionated lymphoid preparations or B-cell-rich fractions. Evidence is presented showing that some types of non-T-cell can inhibit the MF response of T cells. These cells are nonadherent and nonphagocytic and require DNA synthesis to express their inhibitory activity. These results suggest that the regulation of the action of lymphocyte-derived MF is cell-mediated.", "contents": "DNA-synthetic responses of fractionated human lymphocytes exposed to lymphocyte-derived mitogenic factor. Human peripheral lymphocytes that have been exposed to phytohemagglutinin (PHA) release mitogenic factors (MF) on subsequent incubation in fresh medium. In this investigation, MF-rich supernatants were analyzed for their ability to induce DNA synthesis in various fractions of peripheral human lymphocytes. MF was found to induce higher DNA synthesis in T-cell preparations that in unfractionated lymphoid preparations or B-cell-rich fractions. Evidence is presented showing that some types of non-T-cell can inhibit the MF response of T cells. These cells are nonadherent and nonphagocytic and require DNA synthesis to express their inhibitory activity. These results suggest that the regulation of the action of lymphocyte-derived MF is cell-mediated."}
{"id": "PMID:1087747", "title": "Chronic lymphocytic leukemia: studies on the effect of drug treatment on different lymphocytic subpopulations.", "content": "In the present work the effect of drug treatment on different lymphocyte populations in chronic lymphocytic leukemia was studied. During therapy there was evidence of an increased number of cells unidentifiable by conventional surface markers, provisionally termed lymphocyte surface-marker-negative cells. In addition, evidence of increased numbers of T lymphocytes in untreated patients and further evidence of IgG chronic lymphocytic leukemia cells were obtained.", "contents": "Chronic lymphocytic leukemia: studies on the effect of drug treatment on different lymphocytic subpopulations. In the present work the effect of drug treatment on different lymphocyte populations in chronic lymphocytic leukemia was studied. During therapy there was evidence of an increased number of cells unidentifiable by conventional surface markers, provisionally termed lymphocyte surface-marker-negative cells. In addition, evidence of increased numbers of T lymphocytes in untreated patients and further evidence of IgG chronic lymphocytic leukemia cells were obtained."}
{"id": "PMID:1087748", "title": "Suppressor cell activity in a male infant with T-and B-lymphocyte dysfunction treated with thymosin.", "content": "A male infant with bilateral iris coloboma who had had repeated infections and malabsorption was studied. The levels of total lymphocytes and of T and B cells were normal or high, but IgA became undectable and IgG low, whereas IgM was normal. His lymphocytes did not respond to phytohemagglutinin (PHA), concanavalin A, pokeweed mitogen (PWM) or in mixed lymphocyte reactions (MLR), nor did they respond in vitro when thymosin was included in the test systems. He was skin-test-negative, even to dinitrochlorobenzene. His crudely isolated T lymphocytes and the supernatant of his PHA-stimulated lymphocytes inhibit the response of normal lymphocytes to PHA, PWM, and in MLR. During thymosin treatment skin test and lymphocyte reactivity to mitogen remained negative. He became faintly positive in MLR, and the suppressor activity in the supernatant of his PHA-stimulated lymphocytes no longer inhibited the response of normal lymphocytes to PHA, PWM, or in MLR. In parallel with thymosin treatment he showed quite marked clinical improvement.", "contents": "Suppressor cell activity in a male infant with T-and B-lymphocyte dysfunction treated with thymosin. A male infant with bilateral iris coloboma who had had repeated infections and malabsorption was studied. The levels of total lymphocytes and of T and B cells were normal or high, but IgA became undectable and IgG low, whereas IgM was normal. His lymphocytes did not respond to phytohemagglutinin (PHA), concanavalin A, pokeweed mitogen (PWM) or in mixed lymphocyte reactions (MLR), nor did they respond in vitro when thymosin was included in the test systems. He was skin-test-negative, even to dinitrochlorobenzene. His crudely isolated T lymphocytes and the supernatant of his PHA-stimulated lymphocytes inhibit the response of normal lymphocytes to PHA, PWM, and in MLR. During thymosin treatment skin test and lymphocyte reactivity to mitogen remained negative. He became faintly positive in MLR, and the suppressor activity in the supernatant of his PHA-stimulated lymphocytes no longer inhibited the response of normal lymphocytes to PHA, PWM, or in MLR. In parallel with thymosin treatment he showed quite marked clinical improvement."}
{"id": "PMID:1087749", "title": "Lung function studies in asymptomatic individuals with moderately (Pi SZ) and severely (Pi Z) reduced levels of alpha1-antitrypsin.", "content": "Extensive physiological evaluation of lung function were conducted in two groups of asymptomatic individuals with deficiencies of alpha1-antitrypsin: seven subjects with phenotype Pi SZ and six subjects with phenotype Pi Z. Four symptomatic individuals of Pi Z type were also investigated. The asymptomatic individuals of both SZ and Z phenotypes revealed reduction of elastic recoil, hypoventilation and hypoperfusion of the lung bases with increased closing and nitogen wash-out volumes. No signs of bronchial obstruction were observed. The asymptomatic Pi SZ subjects revealed as many signs of subclinical disease as did the asymptomatic Pi Z subjects. These data suggest that Pi SZ subjects are at increased risk of emphysema similar to Pi Z subjects.", "contents": "Lung function studies in asymptomatic individuals with moderately (Pi SZ) and severely (Pi Z) reduced levels of alpha1-antitrypsin. Extensive physiological evaluation of lung function were conducted in two groups of asymptomatic individuals with deficiencies of alpha1-antitrypsin: seven subjects with phenotype Pi SZ and six subjects with phenotype Pi Z. Four symptomatic individuals of Pi Z type were also investigated. The asymptomatic individuals of both SZ and Z phenotypes revealed reduction of elastic recoil, hypoventilation and hypoperfusion of the lung bases with increased closing and nitogen wash-out volumes. No signs of bronchial obstruction were observed. The asymptomatic Pi SZ subjects revealed as many signs of subclinical disease as did the asymptomatic Pi Z subjects. These data suggest that Pi SZ subjects are at increased risk of emphysema similar to Pi Z subjects."}
{"id": "PMID:1087750", "title": "[Life expectancy and frequency of infarct after aorto-coronary bypass].", "content": "Survival rate and incidence of myocardial infarction after aortocoronary bypass operation in 274 patients are presented. Mortality was 5.1% within the first postoperative month and the 5-year-survival rate was 86%. There were 24 perioperative myocardial infarctions and another 21 infarctions over the next 5 years. Comparison of these data with the natural history of coronary heart disease suggests a possible prolongation of survival after bypass surgery. The incidence of myocardial infarction appears to be unchanged.", "contents": "[Life expectancy and frequency of infarct after aorto-coronary bypass]. Survival rate and incidence of myocardial infarction after aortocoronary bypass operation in 274 patients are presented. Mortality was 5.1% within the first postoperative month and the 5-year-survival rate was 86%. There were 24 perioperative myocardial infarctions and another 21 infarctions over the next 5 years. Comparison of these data with the natural history of coronary heart disease suggests a possible prolongation of survival after bypass surgery. The incidence of myocardial infarction appears to be unchanged."}
{"id": "PMID:1087751", "title": "[Work load hemodynamics before and after aortocoronary bypass].", "content": "Left ventricular enddiastolic pressure (LVEDP) was studied during exercise in 22 patients pre- and postoperatively under an identical work load. 13 patients showed improvement of LVEDP (normalization in 5), LVEDP was unchanged in 5 (w.n.l. in 2), and had deteriorated in 4. These results suggest that improvement or normalization of LVEDP under exercise following aortocoronary bypass surgery can be assumed if there is complete revascularization, if all grafts are functioning well, if there is no progression of the underlying disease, and if preoperative LV angiography is normal or shows only ischemic (reversible) hypokinesis.", "contents": "[Work load hemodynamics before and after aortocoronary bypass]. Left ventricular enddiastolic pressure (LVEDP) was studied during exercise in 22 patients pre- and postoperatively under an identical work load. 13 patients showed improvement of LVEDP (normalization in 5), LVEDP was unchanged in 5 (w.n.l. in 2), and had deteriorated in 4. These results suggest that improvement or normalization of LVEDP under exercise following aortocoronary bypass surgery can be assumed if there is complete revascularization, if all grafts are functioning well, if there is no progression of the underlying disease, and if preoperative LV angiography is normal or shows only ischemic (reversible) hypokinesis."}
{"id": "PMID:1087752", "title": "[\"Impending infarct\". Clinical, ergometric and angiographic pre- and postoperative results in 12 emergency operated patients. Preliminary report].", "content": "Pre- and postoperative results are presented in 12 patients who underwent emergency aortocoronary bypass. Operative mortality was zero. 1 patient died 8 months after surgery. Postoperative follow-up averaged 12.5 months (2-37 months). Postoperatively, 7 patients were totally angina-free, 1 was considerably improved and 3 were unchanged.", "contents": "[\"Impending infarct\". Clinical, ergometric and angiographic pre- and postoperative results in 12 emergency operated patients. Preliminary report]. Pre- and postoperative results are presented in 12 patients who underwent emergency aortocoronary bypass. Operative mortality was zero. 1 patient died 8 months after surgery. Postoperative follow-up averaged 12.5 months (2-37 months). Postoperatively, 7 patients were totally angina-free, 1 was considerably improved and 3 were unchanged."}
{"id": "PMID:1087753", "title": "[Left ventricular function at rest and during dynamic load before and after aortocoronary bypass. Preliminary report].", "content": "17 patients with coronary artery disease were studied before and 10 +/- 3 months (mean +/- 1 SD) after aortocoronary bypass surgery. Left ventricular performance was analyzed at rest and in 12 cases during dynamic exercise (work load 59 +/- 22 watts) with tip manometer pressure measurements. The degree of coronary artery obstruction was estimated pre- and postoperatively by vascularization index (VaI). Patients were separated into 2 groups (group A: 8 patients with postoperatively improved VaI, and group B: 9 patients with unchanged or worsened VaI). Apart from a significant fall in LVEDP (p less than 0.025) in group A, there was no significant change in left ventricular dynamics in either group at rest, while a significant improvement in mean LVEDP (p less than 0.05), max dP/dt (p less than 0.05), Vpm (p less than 0.005) and Vmax (p less than 0.005) during dynamic exercise was observed in group A, but no significant change in these terms in group B, after surgery.", "contents": "[Left ventricular function at rest and during dynamic load before and after aortocoronary bypass. Preliminary report]. 17 patients with coronary artery disease were studied before and 10 +/- 3 months (mean +/- 1 SD) after aortocoronary bypass surgery. Left ventricular performance was analyzed at rest and in 12 cases during dynamic exercise (work load 59 +/- 22 watts) with tip manometer pressure measurements. The degree of coronary artery obstruction was estimated pre- and postoperatively by vascularization index (VaI). Patients were separated into 2 groups (group A: 8 patients with postoperatively improved VaI, and group B: 9 patients with unchanged or worsened VaI). Apart from a significant fall in LVEDP (p less than 0.025) in group A, there was no significant change in left ventricular dynamics in either group at rest, while a significant improvement in mean LVEDP (p less than 0.05), max dP/dt (p less than 0.05), Vpm (p less than 0.005) and Vmax (p less than 0.005) during dynamic exercise was observed in group A, but no significant change in these terms in group B, after surgery."}
{"id": "PMID:1087754", "title": "[Study of cellular immunology in malignant and benign monoclonal gammapathies].", "content": "In 25 cases of myeloma and 15 cases of benign paraproteinemia the functional situation of circulating lymphocytes was studied. The technique used was the incorporation of 3H-thymidine after PHA stimulation. E rosette formation and immunoglobulin bearing cells were evaluated. A reduction of 3H-thymidine incorporation in the blastic cells was seen in the patients with myeloma (the difference was statistically significant: p less than 0.001). In benign monoclonal gammopathies the reduction was moderate and was not statistically significant. The different blastic response in vitro in the two group of patients tested confirms that the respective immunocompetent systems clearly differ.", "contents": "[Study of cellular immunology in malignant and benign monoclonal gammapathies]. In 25 cases of myeloma and 15 cases of benign paraproteinemia the functional situation of circulating lymphocytes was studied. The technique used was the incorporation of 3H-thymidine after PHA stimulation. E rosette formation and immunoglobulin bearing cells were evaluated. A reduction of 3H-thymidine incorporation in the blastic cells was seen in the patients with myeloma (the difference was statistically significant: p less than 0.001). In benign monoclonal gammopathies the reduction was moderate and was not statistically significant. The different blastic response in vitro in the two group of patients tested confirms that the respective immunocompetent systems clearly differ."}
{"id": "PMID:1087759", "title": "[Natamycin-S treatment of ringworm in cattle. Epidemiological aspects (author's transl)].", "content": "At a test centre for bulls the progress of a ringworm infection was studied. By treating only the affected animals with natamycin-S the progress of the disease as well as the spreading of the infection among untreated animals could be studied. By treating only the affected animals with natamycin-S the progress of the disease as well as information could be gathered on the difference of progress of the infection in both groups and also of the role of possible immunity.", "contents": "[Natamycin-S treatment of ringworm in cattle. Epidemiological aspects (author's transl)]. At a test centre for bulls the progress of a ringworm infection was studied. By treating only the affected animals with natamycin-S the progress of the disease as well as the spreading of the infection among untreated animals could be studied. By treating only the affected animals with natamycin-S the progress of the disease as well as information could be gathered on the difference of progress of the infection in both groups and also of the role of possible immunity."}
{"id": "PMID:1087761", "title": "Serological identification of Ia antigens: report of a British region Ia workshop.", "content": "In preparation for the 7th International Histocompatibility Workshop 13 laboratories in the British Region participated in a local workshop. One hundred and twenty-three sera which had been previously shown to have activity on either normal B cells, CLL cells or B cell lymphoid lines in the absence of HLA-A, B or C activity were exchanged between the laboratories. These sera were tested on a total of 212 B cells, 101 CLL cells, 76 T cells and 76 lymphoid cell lines. The data was collected and analyzed in Oxford. The analysis showed that six groups of sera could be distinguished. When these groups were compared with the D locus typing of some of the lymphoid lines which were derived from individuals used as MLC typing cells, they were seen to have significant associations with D locus antigens. The serological groups defined were therefore given numbers corresponding to the D locus numbers they associate with, i.e. UK1 is associated with DW1 and so on for UK2, 3, 4, 5 and 7. Comparison of typing techniques showed that long incubation both with antiserum and then with complement, 1 hour + 2 hours gave the best and most reproducible reactions on normal B cells. Residual anti-HLA-A, B or C activity in some of the sera even after platelet absorption showed the importance of adequate checking on T cells after absorption.", "contents": "Serological identification of Ia antigens: report of a British region Ia workshop. In preparation for the 7th International Histocompatibility Workshop 13 laboratories in the British Region participated in a local workshop. One hundred and twenty-three sera which had been previously shown to have activity on either normal B cells, CLL cells or B cell lymphoid lines in the absence of HLA-A, B or C activity were exchanged between the laboratories. These sera were tested on a total of 212 B cells, 101 CLL cells, 76 T cells and 76 lymphoid cell lines. The data was collected and analyzed in Oxford. The analysis showed that six groups of sera could be distinguished. When these groups were compared with the D locus typing of some of the lymphoid lines which were derived from individuals used as MLC typing cells, they were seen to have significant associations with D locus antigens. The serological groups defined were therefore given numbers corresponding to the D locus numbers they associate with, i.e. UK1 is associated with DW1 and so on for UK2, 3, 4, 5 and 7. Comparison of typing techniques showed that long incubation both with antiserum and then with complement, 1 hour + 2 hours gave the best and most reproducible reactions on normal B cells. Residual anti-HLA-A, B or C activity in some of the sera even after platelet absorption showed the importance of adequate checking on T cells after absorption."}
{"id": "PMID:1087766", "title": "Uveitis. A series of 368 patients.", "content": "During the first 10 years (1963-74) of a new Medical Ophthalmology Unit, 368 of 3,930 admissions had endogenous uveitis, anterior in 215 (58 per cent.), posterior in 65 (18 per cent.), and generalized in 88 (24 per cent.). Acute uveitis in 153 patients tended to have a short course counted in months, whereas in 215 with chronic uveitis the condition was measured over years, punctuated by relapses. Sex distribution was equal. At the time of initial presentation, 60 per cent. of patients were aged 20 to 40 years, 8 per cent. were under 20, and 10 per cent. were over 60 years of age.", "contents": "Uveitis. A series of 368 patients. During the first 10 years (1963-74) of a new Medical Ophthalmology Unit, 368 of 3,930 admissions had endogenous uveitis, anterior in 215 (58 per cent.), posterior in 65 (18 per cent.), and generalized in 88 (24 per cent.). Acute uveitis in 153 patients tended to have a short course counted in months, whereas in 215 with chronic uveitis the condition was measured over years, punctuated by relapses. Sex distribution was equal. At the time of initial presentation, 60 per cent. of patients were aged 20 to 40 years, 8 per cent. were under 20, and 10 per cent. were over 60 years of age."}
{"id": "PMID:1087767", "title": "Neglected lid deformities causing progressive corneal disease. Surgical correction of entropion, trichiasis, marginal keratinization, and functional lid shortening.", "content": "The potentially blinding abrasive deformities of the lids--trichiasis, districhiasis, entropion, and marginal keratinization--and the defects in lid closure resulting from functionally shortened upper or lower lids should be corrected surgically before contact lenses are fitted or corneal grafting undertaken. The choice of procedure for surgical correction of various degrees of trichiasis-entropion is indicated by the various associated findings, such as thickness of tarsal plate, presence of functional shortening of the upper lid, and involvement of the lower lid (Table II).", "contents": "Neglected lid deformities causing progressive corneal disease. Surgical correction of entropion, trichiasis, marginal keratinization, and functional lid shortening. The potentially blinding abrasive deformities of the lids--trichiasis, districhiasis, entropion, and marginal keratinization--and the defects in lid closure resulting from functionally shortened upper or lower lids should be corrected surgically before contact lenses are fitted or corneal grafting undertaken. The choice of procedure for surgical correction of various degrees of trichiasis-entropion is indicated by the various associated findings, such as thickness of tarsal plate, presence of functional shortening of the upper lid, and involvement of the lower lid (Table II)."}
{"id": "PMID:1087769", "title": "[Effect of cold acclimatization of grass frogs on the heat resistance of their somatic muscles in summer].", "content": "During cold acclimation (+5 degrees C) of the grass frog Rana temporaria L. changes in heat resistance were observed for six of seven skeletal muscles examined. Changes in the resistance of different muscles were directed differently. Low-resistant muscles demonstrated an increased resistance, wheras high-resistant muscles showed a decrease resistance. Within 24 hours of acclimation, all the muscles demonstrated totally decreased heat resistance. By the end of acclimation (30 days), the resistance of muscles increased to approach the initial level. On the background of those situation changes, seasonal changes in resistance of muscles proceeded.", "contents": "[Effect of cold acclimatization of grass frogs on the heat resistance of their somatic muscles in summer]. During cold acclimation (+5 degrees C) of the grass frog Rana temporaria L. changes in heat resistance were observed for six of seven skeletal muscles examined. Changes in the resistance of different muscles were directed differently. Low-resistant muscles demonstrated an increased resistance, wheras high-resistant muscles showed a decrease resistance. Within 24 hours of acclimation, all the muscles demonstrated totally decreased heat resistance. By the end of acclimation (30 days), the resistance of muscles increased to approach the initial level. On the background of those situation changes, seasonal changes in resistance of muscles proceeded."}
{"id": "PMID:1087771", "title": "[Relationship between the heat resistance of different muscles and frog viability during heat selection].", "content": "An attempt was made to elucidate the relation between the survival of mature frogs Rana temporaria during sudden (unforeseen) thermal selection and the heat resistance of several muscles: m. interfalangealis, m. ileofibularis, m. sartorius, m. gastrocnemius and m. rectus abdominis. It has been found that of greater selective value are the individuals with lower heat resistance not in all muscles, but in those having lesser heat resistance, such as m. ileofibularis and m. sartorius.", "contents": "[Relationship between the heat resistance of different muscles and frog viability during heat selection]. An attempt was made to elucidate the relation between the survival of mature frogs Rana temporaria during sudden (unforeseen) thermal selection and the heat resistance of several muscles: m. interfalangealis, m. ileofibularis, m. sartorius, m. gastrocnemius and m. rectus abdominis. It has been found that of greater selective value are the individuals with lower heat resistance not in all muscles, but in those having lesser heat resistance, such as m. ileofibularis and m. sartorius."}
{"id": "PMID:1087772", "title": "[Cell response to penetration by informational macromolecules].", "content": "A relation is shown between the kinetic for of DNA uptake by the thymocytes (extremal or monotonous), the level of uptake (low or high) and the origin of DNA (hetero-or homologous). Preliminary irradiation of cells promotes a manifestation of  a cytopathogenous effect of the heterologous DNA. The association between the cytopathogenous effect of DNA and that of its information characteristics has been confirmed. The demonstration of cytopathogenous effects of native and degraded DNA was studied as a function of time of their storage. The chromatographic analysis of dispersion of the molecular weights of preparations at different time of their starage allowed a conclusion of the important role played by structure defects in manifestation of cytopathogenous properties of DNA.", "contents": "[Cell response to penetration by informational macromolecules]. A relation is shown between the kinetic for of DNA uptake by the thymocytes (extremal or monotonous), the level of uptake (low or high) and the origin of DNA (hetero-or homologous). Preliminary irradiation of cells promotes a manifestation of  a cytopathogenous effect of the heterologous DNA. The association between the cytopathogenous effect of DNA and that of its information characteristics has been confirmed. The demonstration of cytopathogenous effects of native and degraded DNA was studied as a function of time of their storage. The chromatographic analysis of dispersion of the molecular weights of preparations at different time of their starage allowed a conclusion of the important role played by structure defects in manifestation of cytopathogenous properties of DNA."}
{"id": "PMID:1087773", "title": "Polyarthritis, hepatitis and anti-native DNA antibodies after treatment with ethambutol and rifampicin.", "content": "After seven months' continuous treatment for suspected tuberculosis with rifampicin and ethambutol a nine-year-old boy developed polyarthritis, rash and hepatitis in association with anti-native DNA antibodies and positive antinuclear factor. Six weeks after withdrawal of the antituberculosis drugs and conservative management, the boy was clinically well and ten months later he remained well clinically and liver function tests, anti-DNA antibody and antinuclear factor tests were normal.", "contents": "Polyarthritis, hepatitis and anti-native DNA antibodies after treatment with ethambutol and rifampicin. After seven months' continuous treatment for suspected tuberculosis with rifampicin and ethambutol a nine-year-old boy developed polyarthritis, rash and hepatitis in association with anti-native DNA antibodies and positive antinuclear factor. Six weeks after withdrawal of the antituberculosis drugs and conservative management, the boy was clinically well and ten months later he remained well clinically and liver function tests, anti-DNA antibody and antinuclear factor tests were normal."}
{"id": "PMID:1087775", "title": "[Effectiveness of vagotomy after repeated operations in duodenal ulcer].", "content": "The results of surgical therapy in 24 patients in whom reoperations on the stomach were supplemented with vagotomy are analysed. It is felt that in complicated peptic ulcers of gastroenteroanastomosis in operations after suturing perforated duodenal ulcer, in erosive gastritis of the gastric stump with hemorrhages reoperations on the stomach should be associated with vagotomy.", "contents": "[Effectiveness of vagotomy after repeated operations in duodenal ulcer]. The results of surgical therapy in 24 patients in whom reoperations on the stomach were supplemented with vagotomy are analysed. It is felt that in complicated peptic ulcers of gastroenteroanastomosis in operations after suturing perforated duodenal ulcer, in erosive gastritis of the gastric stump with hemorrhages reoperations on the stomach should be associated with vagotomy."}
{"id": "PMID:1087794", "title": "[Dissecatio profunda ventriculi as an uncommon complication of necrotizing pancreatitis (author's transl)].", "content": "Case report on an uncommon complication of a chronic-relapsing pancreatitis with a subacute necrotizing recidive and a retrogastrically located parapancreatic pseudocyst in a 44-yr-old man. An arrosion bleeding out of the A. lienalis into the pseudocyst with a pressure-induced perforation of the cyst led to a rupture of the outer layers of the wall of the stomach with a following splitting of the posterior gastric wall in the middle of the tunica muscularis in an extension of 30 x 10 cm. A rerupture into the abdominal cavity with an internal bleeding caused the death.", "contents": "[Dissecatio profunda ventriculi as an uncommon complication of necrotizing pancreatitis (author's transl)]. Case report on an uncommon complication of a chronic-relapsing pancreatitis with a subacute necrotizing recidive and a retrogastrically located parapancreatic pseudocyst in a 44-yr-old man. An arrosion bleeding out of the A. lienalis into the pseudocyst with a pressure-induced perforation of the cyst led to a rupture of the outer layers of the wall of the stomach with a following splitting of the posterior gastric wall in the middle of the tunica muscularis in an extension of 30 x 10 cm. A rerupture into the abdominal cavity with an internal bleeding caused the death."}
{"id": "PMID:1087792", "title": "[Effect of an excess of serotonin in the brain on consolidation of temporary connections].", "content": "As a result of intravenous administration of 100 mg/kg of 5-oxytryptophane (5-OTP) to mice, the serotonin content in the brain rises in ten minutes by 35 to 37 per cent, and in an hour, by 58 to 60 per cent, and declines in a day to its initial level. In case of elaboration of passive avoidance reflexes (PAR) an hour after 5-OTP injection, their preservation considerably decreases in a day's time, while 5-OTP administration immediately following PARs elaboration does not affect their preservation. However, 5-OTP injected after PARs elaboration to animals subjected to preliminary cooling, sharply deteriorates their preservation. These facts in conjunction with the previously obtained date attest that excess of serotonin in the brain has a negative effect on the earliest stages of consolidation. When the animals are cooled and the brain temperature is reduced, the consolidation processes are retarded; as a result, the temporary connections prove to be still vulnerable to the action of serotonin excess set up after PAR elaboration.", "contents": "[Effect of an excess of serotonin in the brain on consolidation of temporary connections]. As a result of intravenous administration of 100 mg/kg of 5-oxytryptophane (5-OTP) to mice, the serotonin content in the brain rises in ten minutes by 35 to 37 per cent, and in an hour, by 58 to 60 per cent, and declines in a day to its initial level. In case of elaboration of passive avoidance reflexes (PAR) an hour after 5-OTP injection, their preservation considerably decreases in a day's time, while 5-OTP administration immediately following PARs elaboration does not affect their preservation. However, 5-OTP injected after PARs elaboration to animals subjected to preliminary cooling, sharply deteriorates their preservation. These facts in conjunction with the previously obtained date attest that excess of serotonin in the brain has a negative effect on the earliest stages of consolidation. When the animals are cooled and the brain temperature is reduced, the consolidation processes are retarded; as a result, the temporary connections prove to be still vulnerable to the action of serotonin excess set up after PAR elaboration."}
{"id": "PMID:1087796", "title": "[Stem cell, T- and B-lymphocyte migration in mouse lines that are high and low reactors to sheep erythrocytes].", "content": "Migration of stem cells and B-lymphocytes from the bone marrow and of T-lymphocytes from the thymus was studied on special models in mice of the CBA and C57BL lines, responding to sheep erythrocytes oppositely. Genetically-determined differences in the height of the immune response between the CBA and C57BL mice in immunization with sheep erythrocytes depended to a certain extent on different expression of the process of intensification of migration of the stem cells, T- and B-lymphocytes in response to the antigen administration.", "contents": "[Stem cell, T- and B-lymphocyte migration in mouse lines that are high and low reactors to sheep erythrocytes]. Migration of stem cells and B-lymphocytes from the bone marrow and of T-lymphocytes from the thymus was studied on special models in mice of the CBA and C57BL lines, responding to sheep erythrocytes oppositely. Genetically-determined differences in the height of the immune response between the CBA and C57BL mice in immunization with sheep erythrocytes depended to a certain extent on different expression of the process of intensification of migration of the stem cells, T- and B-lymphocytes in response to the antigen administration."}
{"id": "PMID:1087793", "title": "[Psychodynamic aspects of severalsheumatic diseases as revealed in tests].", "content": "Three groups of patients with rheumafactor-negative diseases (Palindromic Rheumatism, Reiter's disease, Psoriatic Arthritis) took psychodiagnostic investigations. Tests administrated: MMPI, Rorschach-test, Szondi-test and TAT. The findings  of the qualitative psychoanalytic investigations and interpretations of the  three groups were compared with the psychological topics of patients with chronic Rheumatoid Arthritis as well as with the findings of the psychoanalytic explorations by Zander. Finally some considerations to the link as well as to the reduction of movement responses in the Rorschach-test are made.", "contents": "[Psychodynamic aspects of severalsheumatic diseases as revealed in tests]. Three groups of patients with rheumafactor-negative diseases (Palindromic Rheumatism, Reiter's disease, Psoriatic Arthritis) took psychodiagnostic investigations. Tests administrated: MMPI, Rorschach-test, Szondi-test and TAT. The findings  of the qualitative psychoanalytic investigations and interpretations of the  three groups were compared with the psychological topics of patients with chronic Rheumatoid Arthritis as well as with the findings of the psychoanalytic explorations by Zander. Finally some considerations to the link as well as to the reduction of movement responses in the Rorschach-test are made."}
{"id": "PMID:1087797", "title": "[Production of monospecific serum against secretory IgA (anti-SC) and a standard for quantitative analysis].", "content": "The authors describe a method of obtaining monospecific serum against secretory IgA and the corresponding standard. An immunochemically pure (11.6S) secretory human IgA was extracted from the colostrum by salt fractionation and gel-filtration through Sephadex G-200 and Sepharose 6B; this IgA was used as an antigen for the immunization and the standard for the quantitative determination of SIgA in the secretions. Monospecific anti-SC-serum was obtained by successive exhaustion of the antiserum against the S IgA immunosorbents prepared from normal human serum and the serum of a patient suffering from A myeloma containing polymeric IgA forms.", "contents": "[Production of monospecific serum against secretory IgA (anti-SC) and a standard for quantitative analysis]. The authors describe a method of obtaining monospecific serum against secretory IgA and the corresponding standard. An immunochemically pure (11.6S) secretory human IgA was extracted from the colostrum by salt fractionation and gel-filtration through Sephadex G-200 and Sepharose 6B; this IgA was used as an antigen for the immunization and the standard for the quantitative determination of SIgA in the secretions. Monospecific anti-SC-serum was obtained by successive exhaustion of the antiserum against the S IgA immunosorbents prepared from normal human serum and the serum of a patient suffering from A myeloma containing polymeric IgA forms."}
{"id": "PMID:1087798", "title": "[Effect of antibodies to rat and human cerebral cortex and white matter on heterologous T- and B-cells].", "content": "A study was made of the cytotoxic and complement-fixation activity of the antisera to the cortex and white matter of the rat and human brain upon the mouse and rat thymus and bone marrow cells. The cytotoxicity test proved to be more sensitive and precise. Cytotoxins to rodent thymocytes were revealed on ly in the antisera against the human brain cortex; at the same time they were revealed both in the antisera against the cortex and against the white matter of the rat brain (much more was found in the former). The sera against the rat brain cortex lost their cytotoxicity after the exhaustion with the same antigen, but retained it when the exhaustion was carried out by the white matter.", "contents": "[Effect of antibodies to rat and human cerebral cortex and white matter on heterologous T- and B-cells]. A study was made of the cytotoxic and complement-fixation activity of the antisera to the cortex and white matter of the rat and human brain upon the mouse and rat thymus and bone marrow cells. The cytotoxicity test proved to be more sensitive and precise. Cytotoxins to rodent thymocytes were revealed on ly in the antisera against the human brain cortex; at the same time they were revealed both in the antisera against the cortex and against the white matter of the rat brain (much more was found in the former). The sera against the rat brain cortex lost their cytotoxicity after the exhaustion with the same antigen, but retained it when the exhaustion was carried out by the white matter."}
{"id": "PMID:1087799", "title": "[Clinical electromyography and several problems in the neurophysiology of muscle tonus in man].", "content": "On the basis of a summarization of long-term studies of the different types of tonic reactions in normals and patients with different forms of dystonia, the author describes 7 types of such reactions. The paper deals as well as with possible physiological and pathophysiological mechanisms of their occurrence and the significance of these data for the theoretical analysis of the regulation of the human muscular tone.", "contents": "[Clinical electromyography and several problems in the neurophysiology of muscle tonus in man]. On the basis of a summarization of long-term studies of the different types of tonic reactions in normals and patients with different forms of dystonia, the author describes 7 types of such reactions. The paper deals as well as with possible physiological and pathophysiological mechanisms of their occurrence and the significance of these data for the theoretical analysis of the regulation of the human muscular tone."}
{"id": "PMID:1087800", "title": "[Complex therapy of movement disorders in lesions of the peripheral nervous system].", "content": "The authors conducted comprehensive treatment of 335 patients with traumatical injuries of the nervous trunks and plexi and of 361 patients with sacro-lumbar radiculoneuritis with a disturbances of the peroneus nerve. Positive results were attained in 80-85% of the patients with motor disorders when programmed bioelectrical stimulation was included into the therapeutical process. The possible mechanisms of action in automatized movements and disturbances of the motor functions are discussed.", "contents": "[Complex therapy of movement disorders in lesions of the peripheral nervous system]. The authors conducted comprehensive treatment of 335 patients with traumatical injuries of the nervous trunks and plexi and of 361 patients with sacro-lumbar radiculoneuritis with a disturbances of the peroneus nerve. Positive results were attained in 80-85% of the patients with motor disorders when programmed bioelectrical stimulation was included into the therapeutical process. The possible mechanisms of action in automatized movements and disturbances of the motor functions are discussed."}
{"id": "PMID:1087801", "title": "[Threshold in surveillance of pacemaker wearers].", "content": "Measurement of the threshold is important in the follow-up of pacemaker patients; it permits the assessment of the safety margin, between, on the one hand, the electrical impulse, and, on the other, the possible output of the pacemaker. In fact, one can only rarely obtain relative threshold measurements in the clinical situation using non-invasive techniques; this ability is limited to some units made only by a few pacemaker manufacturers. A decrease in the margin of safety may result from two main causes: an increase in the absolute threshold or a decrease in the pacemaker pulse amplitude due to depletion of the cells, or to electronic failure. If safety margin is reduced, and operative procedure is required to achieve the correct diagnosis by precise measurements of the absolute threshold.", "contents": "[Threshold in surveillance of pacemaker wearers]. Measurement of the threshold is important in the follow-up of pacemaker patients; it permits the assessment of the safety margin, between, on the one hand, the electrical impulse, and, on the other, the possible output of the pacemaker. In fact, one can only rarely obtain relative threshold measurements in the clinical situation using non-invasive techniques; this ability is limited to some units made only by a few pacemaker manufacturers. A decrease in the margin of safety may result from two main causes: an increase in the absolute threshold or a decrease in the pacemaker pulse amplitude due to depletion of the cells, or to electronic failure. If safety margin is reduced, and operative procedure is required to achieve the correct diagnosis by precise measurements of the absolute threshold."}
{"id": "PMID:1087802", "title": "[Pacemakers 16 years later].", "content": "In 1976, 10 years after the first successful implantations, the pacemaker technique is perfectly well accepted. Transvenous placement of the electrode is preferred in 95 % of the cases. Besides the 15 years nuclear power pacers (1970), conventional mercury pacemakers may reach a longevity of 4 to 5 years because of the reduction of the current drain. Lithium iodine seems a very promising source of energy. The mini-pacemakers with various iodine anodes are particularly attractive. The future is probably a well designed medium sized lithium pacemaker lasting more than 7 years. Patient follow-up is very much improved. All pacemakers exhibit an obvious rate reduction when their source of energy runs down. Therefore general practitioner and even the patients may detect this symptom. The main problem remains the lead resistance. The reduction of the frequency of pacemaker replacements and of the medical check-up makes life more simple for the pacemaker patient.", "contents": "[Pacemakers 16 years later]. In 1976, 10 years after the first successful implantations, the pacemaker technique is perfectly well accepted. Transvenous placement of the electrode is preferred in 95 % of the cases. Besides the 15 years nuclear power pacers (1970), conventional mercury pacemakers may reach a longevity of 4 to 5 years because of the reduction of the current drain. Lithium iodine seems a very promising source of energy. The mini-pacemakers with various iodine anodes are particularly attractive. The future is probably a well designed medium sized lithium pacemaker lasting more than 7 years. Patient follow-up is very much improved. All pacemakers exhibit an obvious rate reduction when their source of energy runs down. Therefore general practitioner and even the patients may detect this symptom. The main problem remains the lead resistance. The reduction of the frequency of pacemaker replacements and of the medical check-up makes life more simple for the pacemaker patient."}
{"id": "PMID:1087803", "title": "Pathology of chronic A-V Block.", "content": "Study of 177 cases of permanent A-V block shows idiopathic bilateral bundle branch fibrosis to be the commonest single cause (33%). This entity covers a spectrum of localised loss of conduction fibres in the proximal left bundle branch and bifurcating main bundle (Lev's disease) to moore periphery loss of conduction fibres in the bundle branches alone (Len\u00e8gre's disease). The aetiological factors in idiopathic bundle branch fibrosis are still obscure. Ischaemic damage is responsible for 17% of cases and are usually patients who have survived destruction of the bundle branches in septal infarction. Calcific A-V block is the term applied to destruction of the main bundle by large masses of calcification in the mitral or aortic valve rings and is responsible for 10% of cases of chronic A-V block. The mass of calcium is visible to the naked eye at autopsy or by X ray in life. Cardiomyopathies of all types (except hypertrophic obstructive cardiomyopathy) involve the conduction system and produce 14% of cases of A-V block. The remaining numerous causes of chronic A-V block are individually very rare ranging through tumour involvement, congenital defects, collagen diseases and surgical or traumatic damage.", "contents": "Pathology of chronic A-V Block. Study of 177 cases of permanent A-V block shows idiopathic bilateral bundle branch fibrosis to be the commonest single cause (33%). This entity covers a spectrum of localised loss of conduction fibres in the proximal left bundle branch and bifurcating main bundle (Lev's disease) to moore periphery loss of conduction fibres in the bundle branches alone (Len\u00e8gre's disease). The aetiological factors in idiopathic bundle branch fibrosis are still obscure. Ischaemic damage is responsible for 17% of cases and are usually patients who have survived destruction of the bundle branches in septal infarction. Calcific A-V block is the term applied to destruction of the main bundle by large masses of calcification in the mitral or aortic valve rings and is responsible for 10% of cases of chronic A-V block. The mass of calcium is visible to the naked eye at autopsy or by X ray in life. Cardiomyopathies of all types (except hypertrophic obstructive cardiomyopathy) involve the conduction system and produce 14% of cases of A-V block. The remaining numerous causes of chronic A-V block are individually very rare ranging through tumour involvement, congenital defects, collagen diseases and surgical or traumatic damage."}
{"id": "PMID:1087807", "title": "[Treatment by electrostimulation of chronic atrioventricular block: surveillance and future perspectives. Introduction].", "content": "After having reviewed the indications and the main modes of electrostimulation, the author introduces the problem of choice and especially of replacement of the pacemaker. In an overwhelming majority of cases, effective stimulation stops because the batteries are exhausted: this is announced in various ways, one of them being a reduced rate of discharge. The author's opinion is that a pacemaker should not be replaced preventively. On the contrary, appropriate follow-up during the last theoretical period of life, as mentioned by the manufacturers, will avoid losing many months of adequate stimulation. This problem will be developed in detail in the next papers.", "contents": "[Treatment by electrostimulation of chronic atrioventricular block: surveillance and future perspectives. Introduction]. After having reviewed the indications and the main modes of electrostimulation, the author introduces the problem of choice and especially of replacement of the pacemaker. In an overwhelming majority of cases, effective stimulation stops because the batteries are exhausted: this is announced in various ways, one of them being a reduced rate of discharge. The author's opinion is that a pacemaker should not be replaced preventively. On the contrary, appropriate follow-up during the last theoretical period of life, as mentioned by the manufacturers, will avoid losing many months of adequate stimulation. This problem will be developed in detail in the next papers."}
{"id": "PMID:1087808", "title": "[Activity of artificial pacemaker surveillance units at the H\u00f4pital Brugmann].", "content": "1. The pacemaker clinic is of invaluable help in the follow-up of patients with implanted cardiac pacemakers.  2. Photoanalysis provides important information in the study of functionating pacemakers and electrodes.", "contents": "[Activity of artificial pacemaker surveillance units at the H\u00f4pital Brugmann]. 1. The pacemaker clinic is of invaluable help in the follow-up of patients with implanted cardiac pacemakers.  2. Photoanalysis provides important information in the study of functionating pacemakers and electrodes."}
{"id": "PMID:1087809", "title": "Results of follow-up study in cardiac pacing at St. Raphael University Hosital in Leuven.", "content": "After this follow-up period some data seem important for the future: 1. Sudden failure never occurred for ventricular asynchronous units. Moreover battery survival time of fixed rate units is longer than that of ventricular-inhibited units. On the other hand, the use of fixed rate pacers avoids a number of demand function-related electrode difficulties. We must be aware of false signals generated by malfunctioning electrodes. 2. The reliability of a pacemaker clinic improves by replacing ventricular-inhibited units after 34 months. 3. Despite the additional work and cost we feel that photoanalysis is indispensable in today's pacemaker clinic.", "contents": "Results of follow-up study in cardiac pacing at St. Raphael University Hosital in Leuven. After this follow-up period some data seem important for the future: 1. Sudden failure never occurred for ventricular asynchronous units. Moreover battery survival time of fixed rate units is longer than that of ventricular-inhibited units. On the other hand, the use of fixed rate pacers avoids a number of demand function-related electrode difficulties. We must be aware of false signals generated by malfunctioning electrodes. 2. The reliability of a pacemaker clinic improves by replacing ventricular-inhibited units after 34 months. 3. Despite the additional work and cost we feel that photoanalysis is indispensable in today's pacemaker clinic."}
{"id": "PMID:1087811", "title": "[Malfunctions of pacemakers].", "content": "No pacemaker is absolutely reliable : more or less threatening failures can be observed after implantation. Pacemaker runaway has completely disappeared. Sudden battery failure, intermittent or permanent, remains too frequent. Other incidents, less frequent or severe, are also reviewed. Failures are often isolated and sporadic, but can sometimes involve a complete manufacture series. It is a medical duty  to follow-up carefully the patients and to announce the failures with a view to permit early recognition of imperfect series. Pacing failures do not play a major role in the mortality in the group of patients with permanently implanted pacemakers. Nevertheless, anticipated replacement of pacemakers increases the cost of the technique.", "contents": "[Malfunctions of pacemakers]. No pacemaker is absolutely reliable : more or less threatening failures can be observed after implantation. Pacemaker runaway has completely disappeared. Sudden battery failure, intermittent or permanent, remains too frequent. Other incidents, less frequent or severe, are also reviewed. Failures are often isolated and sporadic, but can sometimes involve a complete manufacture series. It is a medical duty  to follow-up carefully the patients and to announce the failures with a view to permit early recognition of imperfect series. Pacing failures do not play a major role in the mortality in the group of patients with permanently implanted pacemakers. Nevertheless, anticipated replacement of pacemakers increases the cost of the technique."}
{"id": "PMID:1087812", "title": "[Calculation of the mean pressure of the pulmonary artery and of the valve surface by ECG in mitral stenosis].", "content": "From a group of 50 patients affected by pure mitral stenosis with sinus rhythm, we have tried to obtain multiple correlation equations which enable in to calculate, in each particular case, the mean pressure of the pulmonary artery and mitral valvular area from purely electrocardiographic facts. In an additional group of 16 patients we have been able to verify the mentioned equations, placing all the calculated points within the presumed limits of tolerance. So it appears that the electrocardiographic calculation of these hemodynamic parameters is possible, with sufficient approximation for clinical requirements.", "contents": "[Calculation of the mean pressure of the pulmonary artery and of the valve surface by ECG in mitral stenosis]. From a group of 50 patients affected by pure mitral stenosis with sinus rhythm, we have tried to obtain multiple correlation equations which enable in to calculate, in each particular case, the mean pressure of the pulmonary artery and mitral valvular area from purely electrocardiographic facts. In an additional group of 16 patients we have been able to verify the mentioned equations, placing all the calculated points within the presumed limits of tolerance. So it appears that the electrocardiographic calculation of these hemodynamic parameters is possible, with sufficient approximation for clinical requirements."}
{"id": "PMID:1087813", "title": "[A radioisotope method of measuring pulmonary vascularization].", "content": "The study of regional perfusion of the lungs by a radioisotopic method using a multiple detectors system and 133 Xenon as radioactive tracer, seems to us, an interesting tool in the investigation of valvular or congenital cardiopathy. This method can be repeated as much as necessary and is not traumatic. The reproductibility is good. The radiation exposure is minimal as well for the patient as for the physician.", "contents": "[A radioisotope method of measuring pulmonary vascularization]. The study of regional perfusion of the lungs by a radioisotopic method using a multiple detectors system and 133 Xenon as radioactive tracer, seems to us, an interesting tool in the investigation of valvular or congenital cardiopathy. This method can be repeated as much as necessary and is not traumatic. The reproductibility is good. The radiation exposure is minimal as well for the patient as for the physician."}
{"id": "PMID:1087814", "title": "[Anatomy of the conduction system in an unusual case of a heart ventricle with double inlet (straddling tricuspid valve)].", "content": "The histological study of the conduction system has been done in a case of \"straddling tricuspid valve\". The main anomalies observed were an AV node located more anteriorly but at the left of the posterior interventricular septum and an hypertrophic bifasicular right bundle branch with an unusual anterior radiation. These morphological features allow to discuss the embryology of the conduction system and of the interventricular septum in that congenital cardiopathy.", "contents": "[Anatomy of the conduction system in an unusual case of a heart ventricle with double inlet (straddling tricuspid valve)]. The histological study of the conduction system has been done in a case of \"straddling tricuspid valve\". The main anomalies observed were an AV node located more anteriorly but at the left of the posterior interventricular septum and an hypertrophic bifasicular right bundle branch with an unusual anterior radiation. These morphological features allow to discuss the embryology of the conduction system and of the interventricular septum in that congenital cardiopathy."}
{"id": "PMID:1087815", "title": "Bidirectional tachycardia a study of five cases.", "content": "Five patients with bidirectional tachycardia due to digitalis toxicity associated with severe organic heart disease were studied. The origin of the abnormal rhythm was established with the aid of His bundle recordings in three cases and by indirect clues in the others two. In three cases the origin of bidirectional tachycardia was suprahisian while in two patients it was infrahisian. In one patient the transition from junctional to ventricular tachycardia could be observed. Bidirectional tachycardia appears to be a complex arrhythmia in which similar electrocardiographic configuration can be due to different mechanism. Digitalis toxicity was often a causal factor.", "contents": "Bidirectional tachycardia a study of five cases. Five patients with bidirectional tachycardia due to digitalis toxicity associated with severe organic heart disease were studied. The origin of the abnormal rhythm was established with the aid of His bundle recordings in three cases and by indirect clues in the others two. In three cases the origin of bidirectional tachycardia was suprahisian while in two patients it was infrahisian. In one patient the transition from junctional to ventricular tachycardia could be observed. Bidirectional tachycardia appears to be a complex arrhythmia in which similar electrocardiographic configuration can be due to different mechanism. Digitalis toxicity was often a causal factor."}
{"id": "PMID:1087816", "title": "Suicide with aprindine and digoxin.", "content": "A 48 year old man with severe coronary artery disease died approximately 33 hours after swallowing unknown amounts of aprindine, digoxin, furosemide, acenocoumarol, flurazepam, nitrazepam and lorazepam. Blood analysis carried out 40 min. before death showed no hydric or ionic imbalance; blood gases were normal. Serum digoxin concentration was 3.9 ng/ml; plasma concentration of aprindine was 2.5 mug/ml and plasma concentration of desethylaprindine was 386 ng/ml. Patient was profoundly comatose, hypotensive, and the electrocardiogram showed broad and bizarre QRS complexes; patient ultimately died in irreversible secondary ventricular fibrillation and asystole. Aprindine probably played a decisive role in the fatal outcome of this complex drug intoxication.", "contents": "Suicide with aprindine and digoxin. A 48 year old man with severe coronary artery disease died approximately 33 hours after swallowing unknown amounts of aprindine, digoxin, furosemide, acenocoumarol, flurazepam, nitrazepam and lorazepam. Blood analysis carried out 40 min. before death showed no hydric or ionic imbalance; blood gases were normal. Serum digoxin concentration was 3.9 ng/ml; plasma concentration of aprindine was 2.5 mug/ml and plasma concentration of desethylaprindine was 386 ng/ml. Patient was profoundly comatose, hypotensive, and the electrocardiogram showed broad and bizarre QRS complexes; patient ultimately died in irreversible secondary ventricular fibrillation and asystole. Aprindine probably played a decisive role in the fatal outcome of this complex drug intoxication."}
{"id": "PMID:1087817", "title": "Cardiac changes in Thevetia Nerifolia poisoning.", "content": "There is a dearth of literature on phytotoxicology. A case of poisoning with seed of Thevetia Nerifolia is reported here and the neurotoxic and cardiotoxic manifestations are discussed.", "contents": "Cardiac changes in Thevetia Nerifolia poisoning. There is a dearth of literature on phytotoxicology. A case of poisoning with seed of Thevetia Nerifolia is reported here and the neurotoxic and cardiotoxic manifestations are discussed."}
{"id": "PMID:1087818", "title": "Left bundle branch block: a rare manifestation of digitalis intoxication.", "content": "A case of LBBB due to digoxin intoxication is presented. The diagnosis was made by repeated electrocardiograms and demonstration of high serum digoxin level at the time of toxic manifestation. Differential diagnosis of LBBB may also include drugs like digoxin.", "contents": "Left bundle branch block: a rare manifestation of digitalis intoxication. A case of LBBB due to digoxin intoxication is presented. The diagnosis was made by repeated electrocardiograms and demonstration of high serum digoxin level at the time of toxic manifestation. Differential diagnosis of LBBB may also include drugs like digoxin."}
{"id": "PMID:1087819", "title": "[Incidence, development and prognosis of atrio-ventricular and intraventricular conduction disorders in acute myocardial infarct].", "content": "The incidence, the evolution and the prognosis of conduction disturbances associated with myocardial infarction are described in a serie of 450 consecutive unselected patients hospitalized in a coronary care unit. Pacemaking does not seem to influence largely the natural history of myocardial infarction with conduction disturbances, which is essentially determined by myocardial power failure. Indications of insertion of a pacing catheter are presented.", "contents": "[Incidence, development and prognosis of atrio-ventricular and intraventricular conduction disorders in acute myocardial infarct]. The incidence, the evolution and the prognosis of conduction disturbances associated with myocardial infarction are described in a serie of 450 consecutive unselected patients hospitalized in a coronary care unit. Pacemaking does not seem to influence largely the natural history of myocardial infarction with conduction disturbances, which is essentially determined by myocardial power failure. Indications of insertion of a pacing catheter are presented."}
{"id": "PMID:1087820", "title": "Advantages of side-to-side anastomosis in coronary surgery[(author's transl)].", "content": "It is of great importance to perform the most complete possible correction of multiple coronoronary vessels was shown at the Institut de Cardiologie de Montreal: early occlucion of grafts was low (1/32) and early occlusion of one of the anastomoses was acceptable (5/32). At one year follow-up (20 of the 32 grafts reviewed early), no additional total graft thrombosis was noted and only 2 more end-anastomes were lost. These rates of thrombosis are lover than those generally reported for series with a single anastomosis. The diagonal and anterior interventricular branches yield well to this technique; the main deterrent is the occurrence of unfavorable kinking of the graft.", "contents": "Advantages of side-to-side anastomosis in coronary surgery[(author's transl)]. It is of great importance to perform the most complete possible correction of multiple coronoronary vessels was shown at the Institut de Cardiologie de Montreal: early occlucion of grafts was low (1/32) and early occlusion of one of the anastomoses was acceptable (5/32). At one year follow-up (20 of the 32 grafts reviewed early), no additional total graft thrombosis was noted and only 2 more end-anastomes were lost. These rates of thrombosis are lover than those generally reported for series with a single anastomosis. The diagonal and anterior interventricular branches yield well to this technique; the main deterrent is the occurrence of unfavorable kinking of the graft."}
{"id": "PMID:1087825", "title": "[Unilateral caloric hyporeflexis].", "content": "Seventy-one patients with an unilateral caloric hypofunction were examined in order to establish the correlation with cochlear function. We divised our cases following the subjective clinical syndromes. Groupe A (12 patients) with a history of only one crise of vertigo. Groupe B (13 patients) with repeated vertiginous attacks. Groupe C (21 patients) with a positional vertigo only. Groupe D (15 patients) with rather undefinite complaints. Groupe E (7 patients) with no vertigo at all. We believe to discern the following syndromes in our cases of unilateral vestibular hypofunction: 9 cases with a history of a unique vertiginous crises could be considered as a vestibular \"neuronitis\" in the narrow sense of the word. One case with recidiving attacks could be considered as a neuronitis, or as a Meni\u00e8re without cochlear disturbances. Some cases with a positional vertigo only or with very vague complaints could be considered as a neuronitis in a large sense.", "contents": "[Unilateral caloric hyporeflexis]. Seventy-one patients with an unilateral caloric hypofunction were examined in order to establish the correlation with cochlear function. We divised our cases following the subjective clinical syndromes. Groupe A (12 patients) with a history of only one crise of vertigo. Groupe B (13 patients) with repeated vertiginous attacks. Groupe C (21 patients) with a positional vertigo only. Groupe D (15 patients) with rather undefinite complaints. Groupe E (7 patients) with no vertigo at all. We believe to discern the following syndromes in our cases of unilateral vestibular hypofunction: 9 cases with a history of a unique vertiginous crises could be considered as a vestibular \"neuronitis\" in the narrow sense of the word. One case with recidiving attacks could be considered as a neuronitis, or as a Meni\u00e8re without cochlear disturbances. Some cases with a positional vertigo only or with very vague complaints could be considered as a neuronitis in a large sense."}
{"id": "PMID:1087829", "title": "Reliability of chemical tests for fecal occult blood in hospitalized patients.", "content": "In 39 hospitalized patients with suspected gastrointestinal bleeding and given intravenous 51Cr-labeled red cells, reactions of three chemical spot tests for fecal occult blood were compared with the \"true\" blood loss as determined by stool radioassay. Guaiac reagent and orthotolidine (Hematest) tablets were extremely sensitive, but yielded false-positive reaction rates of 72% and 76%, respectively on the 240 stool specimens compared. A modified guaiac test (Hemoccult) exhibited a false positive rate of 12%. Of the 27 patients entering the study due to positive guaiac or Hematest screening tests, 17 (63%) were not bleeding. Hemoccult, approximately 1/4 as sensitive as guaiac and Hematest, could miss lesions with low rates of bleeding unless multiple stools were tested. While barium had no effect, iron therapy or laxatives tended to lower both false-positive and false-negative reactions for all reagents. A positive Hemoccult test usually indicated significant gastrointestinal bleeding and would appear to be the test of choice provided at least 3 stools are tested to minimize false-negative results.", "contents": "Reliability of chemical tests for fecal occult blood in hospitalized patients. In 39 hospitalized patients with suspected gastrointestinal bleeding and given intravenous 51Cr-labeled red cells, reactions of three chemical spot tests for fecal occult blood were compared with the \"true\" blood loss as determined by stool radioassay. Guaiac reagent and orthotolidine (Hematest) tablets were extremely sensitive, but yielded false-positive reaction rates of 72% and 76%, respectively on the 240 stool specimens compared. A modified guaiac test (Hemoccult) exhibited a false positive rate of 12%. Of the 27 patients entering the study due to positive guaiac or Hematest screening tests, 17 (63%) were not bleeding. Hemoccult, approximately 1/4 as sensitive as guaiac and Hematest, could miss lesions with low rates of bleeding unless multiple stools were tested. While barium had no effect, iron therapy or laxatives tended to lower both false-positive and false-negative reactions for all reagents. A positive Hemoccult test usually indicated significant gastrointestinal bleeding and would appear to be the test of choice provided at least 3 stools are tested to minimize false-negative results."}
{"id": "PMID:1087830", "title": "Atheromatous embolization to the stomach: an unusual cause of gastrointestinal bleeding.", "content": "This is a case report of an 80-year-old female with persistent blood loss from what was initially considered to be \"hemorrhagic gastritis.\" Distal gastrectomy showed extensive atheromatous embolism and resultant ischemic lesions of the stomach--the apparent cause of the chronic bleeding. The patient has remained well without further blood loss during a 15-month follow-up period. While a small number of cases of gastric hemorrhage associated with atheromatous embolism have been reported, they all have been diagnosed at autopsy. This case is unusual in that the lesion was successfully delt with by surgical resection of the involved area.", "contents": "Atheromatous embolization to the stomach: an unusual cause of gastrointestinal bleeding. This is a case report of an 80-year-old female with persistent blood loss from what was initially considered to be \"hemorrhagic gastritis.\" Distal gastrectomy showed extensive atheromatous embolism and resultant ischemic lesions of the stomach--the apparent cause of the chronic bleeding. The patient has remained well without further blood loss during a 15-month follow-up period. While a small number of cases of gastric hemorrhage associated with atheromatous embolism have been reported, they all have been diagnosed at autopsy. This case is unusual in that the lesion was successfully delt with by surgical resection of the involved area."}
{"id": "PMID:1087831", "title": "The influence of aspirin on gastrointestinal microbleeding in dogs with gastric ulcers.", "content": "Fecal blood volume was determined daily in 11 dogs with single gastric ulcers. Beginning 11 days after production of the ulcers and dogs received, in crossover fashion, 2 placebo or ordinary 325-mg aspirin tablets orally twice daily during two 7-day treatment periods separated and followed by 5-day periods of no treatment. Mean daily fecal blood volumes of 0.52 and 3.25 ml were observed during periods of treatment with placebo and aspirin, respectively. However, in 7 previous studies in this laboratory a total of 24 normal dogs have received 7-day courses of treatment with 650 mg ordinary aspirin twice daily on 105 occasions; during these 105 treatment periods fecal blood volume averaged 2.90 ml/day. Thus, it is concluded that the effect of ordinary aspirin in dogs with gastric ulcers is essentially the same as the effect in normal dogs, and that there is no tendency for dogs with gastric ulcers to bleed massively in response to aspirin.", "contents": "The influence of aspirin on gastrointestinal microbleeding in dogs with gastric ulcers. Fecal blood volume was determined daily in 11 dogs with single gastric ulcers. Beginning 11 days after production of the ulcers and dogs received, in crossover fashion, 2 placebo or ordinary 325-mg aspirin tablets orally twice daily during two 7-day treatment periods separated and followed by 5-day periods of no treatment. Mean daily fecal blood volumes of 0.52 and 3.25 ml were observed during periods of treatment with placebo and aspirin, respectively. However, in 7 previous studies in this laboratory a total of 24 normal dogs have received 7-day courses of treatment with 650 mg ordinary aspirin twice daily on 105 occasions; during these 105 treatment periods fecal blood volume averaged 2.90 ml/day. Thus, it is concluded that the effect of ordinary aspirin in dogs with gastric ulcers is essentially the same as the effect in normal dogs, and that there is no tendency for dogs with gastric ulcers to bleed massively in response to aspirin."}
{"id": "PMID:1087832", "title": "Polymorphism of human color vision.", "content": "The genetic polymorphism of human color vision is examined within the framework of a photopigment replacement model. An analysis of the X-linked recessive dichromacies and anomalous trichromacies indicates that one source of variability of normal color perception may be the inclusion of several distinct phenotypes in what is usually described as normal color vision. This analysis also reveals the cause of the dominance hierarchy at the protan and deutan loci, the perceptual effects of dosage compensation, and the phenotypes of various compound hemizygotes.", "contents": "Polymorphism of human color vision. The genetic polymorphism of human color vision is examined within the framework of a photopigment replacement model. An analysis of the X-linked recessive dichromacies and anomalous trichromacies indicates that one source of variability of normal color perception may be the inclusion of several distinct phenotypes in what is usually described as normal color vision. This analysis also reveals the cause of the dominance hierarchy at the protan and deutan loci, the perceptual effects of dosage compensation, and the phenotypes of various compound hemizygotes."}
{"id": "PMID:1087833", "title": "Statistical and methodological considerations for vision screening.", "content": "The statistical measures and definitions currently in use for screening programs are discussed. Sensitivity, specificity, false negative, and false positive rates are defined and illustrated. The positive and negative predictive values and the over- and underrefferal rates are also discussed, and an example of these measures is provided. The relationship of rates to prevalence and expected screening results is shown. A method for calculating the standard error of sensitivity and specificity is also given.", "contents": "Statistical and methodological considerations for vision screening. The statistical measures and definitions currently in use for screening programs are discussed. Sensitivity, specificity, false negative, and false positive rates are defined and illustrated. The positive and negative predictive values and the over- and underrefferal rates are also discussed, and an example of these measures is provided. The relationship of rates to prevalence and expected screening results is shown. A method for calculating the standard error of sensitivity and specificity is also given."}
{"id": "PMID:1087835", "title": "Electroretinographic evaluation of a treatment based on retina phosphatides in patients with various types of retinochoroid degeneration.", "content": "The authors treated 16 patients with various types of retinochoroid degeneration with a preparation containing phospholipids extracted from pig retina. The evaluation of the efficiency of the preparation was carried out using adaptoelectroretinography. Exogenous phospholipids caused a statistically significant increase at the level of the scotopic components of the ERG.", "contents": "Electroretinographic evaluation of a treatment based on retina phosphatides in patients with various types of retinochoroid degeneration. The authors treated 16 patients with various types of retinochoroid degeneration with a preparation containing phospholipids extracted from pig retina. The evaluation of the efficiency of the preparation was carried out using adaptoelectroretinography. Exogenous phospholipids caused a statistically significant increase at the level of the scotopic components of the ERG."}
{"id": "PMID:1087836", "title": "[Investigations of constitution in patients with primary amotio retinae (author's transl)].", "content": "In 53 mostly old patients with Amotio retinae (37 femeles and 16 males) the type of constitution, meaning the placement in the variation grade from leptomorph to pyknomorph, was determined by biometrical methods (discrimination analysis). The calculated mean values of the group of patients were compared to the results of a leptomorph and a pyknomorph group as well as those of a control group. On a linear scale from lepto- to pyknomorph the patients could be placed between the controls and the pyknomorphs. The tendency toward pyknomorphy was statistically significant in the male patients only.", "contents": "[Investigations of constitution in patients with primary amotio retinae (author's transl)]. In 53 mostly old patients with Amotio retinae (37 femeles and 16 males) the type of constitution, meaning the placement in the variation grade from leptomorph to pyknomorph, was determined by biometrical methods (discrimination analysis). The calculated mean values of the group of patients were compared to the results of a leptomorph and a pyknomorph group as well as those of a control group. On a linear scale from lepto- to pyknomorph the patients could be placed between the controls and the pyknomorphs. The tendency toward pyknomorphy was statistically significant in the male patients only."}
{"id": "PMID:1087837", "title": "[Early alterations of the conjunctiva after alkali-burning (author's transl)].", "content": "Early alterations of the conjunctiva after alkaliburning (with 0.25 N NaOH) are described. After chemical damage, there is a nearly total loss of conjunctival epithelium, with simultaneous marked edema in the lower conjunctival parts. Alterations of the surface are examined by scanning electronic microscopy (SEM). Within 24 h marked infiltration of round cells into the conjunctival stroma takes place. After burning, we find a rapid decrease in the ATP level in the conjunctiva to about one-tenth of the normal value, whereas glucose and lactate showed only small alterations before and after chemical injury.", "contents": "[Early alterations of the conjunctiva after alkali-burning (author's transl)]. Early alterations of the conjunctiva after alkaliburning (with 0.25 N NaOH) are described. After chemical damage, there is a nearly total loss of conjunctival epithelium, with simultaneous marked edema in the lower conjunctival parts. Alterations of the surface are examined by scanning electronic microscopy (SEM). Within 24 h marked infiltration of round cells into the conjunctival stroma takes place. After burning, we find a rapid decrease in the ATP level in the conjunctiva to about one-tenth of the normal value, whereas glucose and lactate showed only small alterations before and after chemical injury."}
{"id": "PMID:1087838", "title": "[Blood flow through different tissues under clonidine in rabbits (author's transl)].", "content": "The effect of i.v. administered clonidine on the blood flow through different tissues was investigated in six rabbits which had been anaesthetized with NembutalR. Six additional animals served as controls and received an equal quantity of a physiological sodium chloride solution. The blood flow was determined by means of radioactively labelled microspheres just before as well as 15, 30 and 45 min after the administration of the drug or the placebo respectively. In most of the investigated tissues a considerable decrease in blood flow was observed 15 min after the injection of clonidine, whereas no effect was to be seen after administration of sodium chloride. For the first time after 30 min, and even more after 45 min the blood flow diminished as well in the control group. The flow-decreasing effect of clonidine occured in lung, skin, choroid, the small intestine, the triceps muscle, the ciliary body, iris, and white matter, whereas the opposite reaction was observed in liver (hepatic artery), retina and the optic nerve.", "contents": "[Blood flow through different tissues under clonidine in rabbits (author's transl)]. The effect of i.v. administered clonidine on the blood flow through different tissues was investigated in six rabbits which had been anaesthetized with NembutalR. Six additional animals served as controls and received an equal quantity of a physiological sodium chloride solution. The blood flow was determined by means of radioactively labelled microspheres just before as well as 15, 30 and 45 min after the administration of the drug or the placebo respectively. In most of the investigated tissues a considerable decrease in blood flow was observed 15 min after the injection of clonidine, whereas no effect was to be seen after administration of sodium chloride. For the first time after 30 min, and even more after 45 min the blood flow diminished as well in the control group. The flow-decreasing effect of clonidine occured in lung, skin, choroid, the small intestine, the triceps muscle, the ciliary body, iris, and white matter, whereas the opposite reaction was observed in liver (hepatic artery), retina and the optic nerve."}
{"id": "PMID:1087839", "title": "A possible change of refractive index with age and its relevance to chromatic aberration.", "content": "The Purkinje-Sanson images of the eyes were photographed in red and blue light in two groups of subjects, young (19-38 years) and old (52-63 years). Densitometric examination of the negatives showed a significant increase in the amount of red light and a significant decrease in the amount of blue light reflected from the anterior surface of the lens (third image) in the group of old subjects as compared to the group of young persons. There is also a significant decline in the blue light reflected from the posterior surface of the lens (fourth image) but no change in the red light in old versus young eyes. From these results it is suggested that the index of refraction of the lens increases for red (nC) and decreases for blue light (nF) in the aging eye and similarly the index of the vitreous varies differentially in blue and red light. These specific changes in the indices of the lens and vitreous are believed to account for the large decline of axial chromatic aberration of the aging eye. In addition, the examination of the first Purkinje-Sanson image tends to indicate that the index of refraction of the cornea does not vary with age.", "contents": "A possible change of refractive index with age and its relevance to chromatic aberration. The Purkinje-Sanson images of the eyes were photographed in red and blue light in two groups of subjects, young (19-38 years) and old (52-63 years). Densitometric examination of the negatives showed a significant increase in the amount of red light and a significant decrease in the amount of blue light reflected from the anterior surface of the lens (third image) in the group of old subjects as compared to the group of young persons. There is also a significant decline in the blue light reflected from the posterior surface of the lens (fourth image) but no change in the red light in old versus young eyes. From these results it is suggested that the index of refraction of the lens increases for red (nC) and decreases for blue light (nF) in the aging eye and similarly the index of the vitreous varies differentially in blue and red light. These specific changes in the indices of the lens and vitreous are believed to account for the large decline of axial chromatic aberration of the aging eye. In addition, the examination of the first Purkinje-Sanson image tends to indicate that the index of refraction of the cornea does not vary with age."}
{"id": "PMID:1087840", "title": "[Biomicroscopic aspects of normal iris tissue in culture (author's transl)].", "content": "The cells shown were cultivated from iris pieces of 1-5 mg wet weight incubated in TCM 199 after adding 20% calf serum and antibiotics. Different morphologic aspects allowed some coordination of the cells to the corresponding iris layer. Even after 1 year's incubation, fibroblastic stroma cells showed a radiate growth from the insemination point. Pigment cell-like elements were packed with yellowish, bright, and intensely refracting spherical bodies, indicating a specific function. An analogous finding in the human eye after a perforating iris lesion was growth of chromatophores on the anterior lens capsule.", "contents": "[Biomicroscopic aspects of normal iris tissue in culture (author's transl)]. The cells shown were cultivated from iris pieces of 1-5 mg wet weight incubated in TCM 199 after adding 20% calf serum and antibiotics. Different morphologic aspects allowed some coordination of the cells to the corresponding iris layer. Even after 1 year's incubation, fibroblastic stroma cells showed a radiate growth from the insemination point. Pigment cell-like elements were packed with yellowish, bright, and intensely refracting spherical bodies, indicating a specific function. An analogous finding in the human eye after a perforating iris lesion was growth of chromatophores on the anterior lens capsule."}
{"id": "PMID:1087841", "title": "[A description of a method for objective perimetry (author's transl)].", "content": "An infrared-sensitive TV Camera has been fitted into a projection perimeter, which allows to control fixation independent of the particular background illumination. The consensual pupil light reflex, triggered by the test light of the perimeter, is measured and registered with a special TV processor designed for this purpose. The method was tested for its efficiency on four healthy volunteers. The course of sensory and pupillomotor thresholds were measured on the 135 degree meridian of the visual field. Determinations were conducted in the photopic range of 10 asb background illumination with two test points of 27' and 7'. A difference of 10 dB was found between the sensory and pupillomotor thresholds.", "contents": "[A description of a method for objective perimetry (author's transl)]. An infrared-sensitive TV Camera has been fitted into a projection perimeter, which allows to control fixation independent of the particular background illumination. The consensual pupil light reflex, triggered by the test light of the perimeter, is measured and registered with a special TV processor designed for this purpose. The method was tested for its efficiency on four healthy volunteers. The course of sensory and pupillomotor thresholds were measured on the 135 degree meridian of the visual field. Determinations were conducted in the photopic range of 10 asb background illumination with two test points of 27' and 7'. A difference of 10 dB was found between the sensory and pupillomotor thresholds."}
{"id": "PMID:1087842", "title": "[Autoimmunity in chronic inflammatory eye disease (author's transl)].", "content": "Preparations of allogenous and xenogenous retinal rod outer segments (ROS) were found to be adequately antigenic for detection of cellular immunity in human chorioretinitis. The electrophoretic mobility test was used for estimation of delayed-type hypersensitivity. No significant results with ROS were found in patients with panophthalmia or in control subjects. It is assumed that the results reported here in chronic ophthalmic inflammation may be produced by or connected with autoimmune processes.", "contents": "[Autoimmunity in chronic inflammatory eye disease (author's transl)]. Preparations of allogenous and xenogenous retinal rod outer segments (ROS) were found to be adequately antigenic for detection of cellular immunity in human chorioretinitis. The electrophoretic mobility test was used for estimation of delayed-type hypersensitivity. No significant results with ROS were found in patients with panophthalmia or in control subjects. It is assumed that the results reported here in chronic ophthalmic inflammation may be produced by or connected with autoimmune processes."}
{"id": "PMID:1087843", "title": "[The proof of two distinct types of collagen in the fibrils of the vitreous body and zonula fibers (author's transl)].", "content": "Vitreous body fibrils and zonula fibers are analyzed by disc-electrophoresis. In both tissues the presence of collagen is established. The disc-electrophoresis patterns of vitreous body fibrils and zonula fibers show that a different type of collagen is present in each tissue. The absence of the alpha 2-chain indicates that the tissues investigated contain no type I collagen but types consisting of three identical alpha-chains.", "contents": "[The proof of two distinct types of collagen in the fibrils of the vitreous body and zonula fibers (author's transl)]. Vitreous body fibrils and zonula fibers are analyzed by disc-electrophoresis. In both tissues the presence of collagen is established. The disc-electrophoresis patterns of vitreous body fibrils and zonula fibers show that a different type of collagen is present in each tissue. The absence of the alpha 2-chain indicates that the tissues investigated contain no type I collagen but types consisting of three identical alpha-chains."}
{"id": "PMID:1087845", "title": "Regulation of the immune response. I. Suppression of delayed-type hypersensitivity by T cells from mice expressing humoral immunity.", "content": "The ability of horse red blood cell (HRBC)-specific T cells from mice expressing humoral immunity to suppress the induction of HRBC-specific delayed-type hypersensitivity (DTH) was investigated. The transfer of Ig-negative spleen cells, from mice injected 4 days previously with HRBC, completely suppressed the development of DTH in mice treated with cyclophosphamide and sensitized with HRBC. The suppressor cell was found to be lysed by treatment with anti-theta serum and complement. Furthermore, hemocyanin-specific immune T cells were able to suppress the DTH induced to HRBC, provided these two antigens were coupled together. These studies suggest that T cells present under conditions were humoral immunity is induced can suppress DTH and that such cells play an important role in the regulation of the immune response.", "contents": "Regulation of the immune response. I. Suppression of delayed-type hypersensitivity by T cells from mice expressing humoral immunity. The ability of horse red blood cell (HRBC)-specific T cells from mice expressing humoral immunity to suppress the induction of HRBC-specific delayed-type hypersensitivity (DTH) was investigated. The transfer of Ig-negative spleen cells, from mice injected 4 days previously with HRBC, completely suppressed the development of DTH in mice treated with cyclophosphamide and sensitized with HRBC. The suppressor cell was found to be lysed by treatment with anti-theta serum and complement. Furthermore, hemocyanin-specific immune T cells were able to suppress the DTH induced to HRBC, provided these two antigens were coupled together. These studies suggest that T cells present under conditions were humoral immunity is induced can suppress DTH and that such cells play an important role in the regulation of the immune response."}
{"id": "PMID:1087846", "title": "The xenogeneic effect-- evidence for coparticipation of human monocytes and T lymphocytes in the restoration of nude mouse in vitro response to sheep red blood cells.", "content": "The restorative ability of human peripheral blood lymphocyte fractions on nude mouse spleen cell in vitro antibody response to SRBC was studied. Strongly adherent cells (monocytes) enhanced nude cell response, but not to the same extent as the optimal number (10(6)) of unfractionated human peripheral blood lymphocytes. T-depleted cells lost their ability to optimally restore the response, while T-enriched cells showed a definite restorative ability. The recombination of adherent cells with T-enriched cells produced an effect comparable to that of unfractionated cells, both in terms of magnitude and dose-response curve. These data suggest that both monocytes and T cells are necessary for an optimal xenogeneic effect in Mishell-Dutton cultures.", "contents": "The xenogeneic effect-- evidence for coparticipation of human monocytes and T lymphocytes in the restoration of nude mouse in vitro response to sheep red blood cells. The restorative ability of human peripheral blood lymphocyte fractions on nude mouse spleen cell in vitro antibody response to SRBC was studied. Strongly adherent cells (monocytes) enhanced nude cell response, but not to the same extent as the optimal number (10(6)) of unfractionated human peripheral blood lymphocytes. T-depleted cells lost their ability to optimally restore the response, while T-enriched cells showed a definite restorative ability. The recombination of adherent cells with T-enriched cells produced an effect comparable to that of unfractionated cells, both in terms of magnitude and dose-response curve. These data suggest that both monocytes and T cells are necessary for an optimal xenogeneic effect in Mishell-Dutton cultures."}
{"id": "PMID:1087847", "title": "Development of sheep thymus in relation to in utero thymectomy experiments.", "content": "The developing thymus of sheep embryos has been examined from 20 days of gestation onwards. The initial relationships of the thymus to the parathyroid III and the pericardium are maintained so that the thymus develops as an extensive and complex structure consisting of paired cords of tissues on either side of the neck continuous with a single, fused structure in the anterior mediastinum. Histological examination of the developing thymus revealed that thymic lymphopoiesis commences at 36 days of gestation (term = 150 days). The results indicate that conclusions drawn from sheep thymectomy, usually performed after 60 days of gestation, must be interpreted with caution.", "contents": "Development of sheep thymus in relation to in utero thymectomy experiments. The developing thymus of sheep embryos has been examined from 20 days of gestation onwards. The initial relationships of the thymus to the parathyroid III and the pericardium are maintained so that the thymus develops as an extensive and complex structure consisting of paired cords of tissues on either side of the neck continuous with a single, fused structure in the anterior mediastinum. Histological examination of the developing thymus revealed that thymic lymphopoiesis commences at 36 days of gestation (term = 150 days). The results indicate that conclusions drawn from sheep thymectomy, usually performed after 60 days of gestation, must be interpreted with caution."}
{"id": "PMID:1087850", "title": "[Theoretical models of chromatid exchange established after endoreplication analysi].", "content": "In endoreduplicating cells, two thirds of the chromatid exchanges observed after BrdU treatment are symetrical, like on the two elements of the diplochromosome. The other third seems to occur at random on one or the other element. Each double symetrical exchange would result from a single event occurring at the next to last cell cycle before the mitosis observed. The other exchanges would result from last events of the cycle. Thus the frequency of events would be the same at each cell generation. A theoretical model is proposed which explains the mechanism responsible for chromatid exchanges at the DNA level.", "contents": "[Theoretical models of chromatid exchange established after endoreplication analysi]. In endoreduplicating cells, two thirds of the chromatid exchanges observed after BrdU treatment are symetrical, like on the two elements of the diplochromosome. The other third seems to occur at random on one or the other element. Each double symetrical exchange would result from a single event occurring at the next to last cell cycle before the mitosis observed. The other exchanges would result from last events of the cycle. Thus the frequency of events would be the same at each cell generation. A theoretical model is proposed which explains the mechanism responsible for chromatid exchanges at the DNA level."}
{"id": "PMID:1087852", "title": "[Identification by Q and G bands of chromosome anomalies in spontaneous abortion].", "content": "Banding technique were applied to cell lines which had been established earlier from spontaneous human abortions and preserved frozen. Most of the autosomes were shown to be involved in lethal trisomies. As a result of the precise identification of affected chromosomes, the exact time of developmental arrest as well as certain phenotypes could be correlated with the various trisomies.", "contents": "[Identification by Q and G bands of chromosome anomalies in spontaneous abortion]. Banding technique were applied to cell lines which had been established earlier from spontaneous human abortions and preserved frozen. Most of the autosomes were shown to be involved in lethal trisomies. As a result of the precise identification of affected chromosomes, the exact time of developmental arrest as well as certain phenotypes could be correlated with the various trisomies."}
{"id": "PMID:1087853", "title": "Duplication-deficiency of the short arm of chromosome 8 following artificial insemination.", "content": "A chromosomally abnormal child with psychomotor retardation and multiple anomalies, including agenesis of the corpus callosum and cleft palate, was born following artificial insemination by donor. Chromosomal and conventional markers were used to ascertain paternity. Various banding techniques were employed to identify the origin of the extra chromosomal material as most likely a duplication-deficiency of the short arm of chromosome No. 8.", "contents": "Duplication-deficiency of the short arm of chromosome 8 following artificial insemination. A chromosomally abnormal child with psychomotor retardation and multiple anomalies, including agenesis of the corpus callosum and cleft palate, was born following artificial insemination by donor. Chromosomal and conventional markers were used to ascertain paternity. Various banding techniques were employed to identify the origin of the extra chromosomal material as most likely a duplication-deficiency of the short arm of chromosome No. 8."}
{"id": "PMID:1087854", "title": "Transformation of mouse cells by fusion with chronic granulocytic leukemia cells: possible role of human chromosome.", "content": "In vitro properties of somatic cell hybrids between non-malignant B82 mouse fibroblasts and human chronic granulocytic leukemia spleen cells include lack of cell density-dependent growth inhibition and growth in soft agar. These phenotypic expressions of cell transformation appear linked to the retention by the cells of chromosome 7 from the human leukemic genome.", "contents": "Transformation of mouse cells by fusion with chronic granulocytic leukemia cells: possible role of human chromosome. In vitro properties of somatic cell hybrids between non-malignant B82 mouse fibroblasts and human chronic granulocytic leukemia spleen cells include lack of cell density-dependent growth inhibition and growth in soft agar. These phenotypic expressions of cell transformation appear linked to the retention by the cells of chromosome 7 from the human leukemic genome."}
{"id": "PMID:1087855", "title": "Mapping of the gene for glutathione reductase on chromosome 8.", "content": "Red cell glutathione reductase (E-GSR) activity in 3 patients with mos46,XY/47,XY,+8 was higher than the mean value in controls, confirming the previous assignment of the E-GSR locus to chromosome 8. In a infant with a terminal deletion of the short arm of chromosome 8, 46,XX,del(8)(:p21 leads to qter), E-GSR activity was markedly lower than in infant controls. The authors suggest that the E-GSR locus is in the region 8p21 leads to pter.", "contents": "Mapping of the gene for glutathione reductase on chromosome 8. Red cell glutathione reductase (E-GSR) activity in 3 patients with mos46,XY/47,XY,+8 was higher than the mean value in controls, confirming the previous assignment of the E-GSR locus to chromosome 8. In a infant with a terminal deletion of the short arm of chromosome 8, 46,XX,del(8)(:p21 leads to qter), E-GSR activity was markedly lower than in infant controls. The authors suggest that the E-GSR locus is in the region 8p21 leads to pter."}
{"id": "PMID:1087856", "title": "Balanced translocations involving chromosome 12: report of a case and possible evidence for position effect.", "content": "A girl with mental retardation, a convulsive disorder and clinical findings resembling cerebral palsy had an apparently balanced 12/18 translocation. Three additional cases reported in the literature had an abnormal phenotype associated with the positioning of segments from other autosomes adjacent to the band 12q21. Penotype abnormalities were not present in 14 individuals in which the translocation involved breaks at other sites on chromosome no. 12. These findings suggest the possibility of a position effect on chromosome segments translocated adjacent to the region 12q21.", "contents": "Balanced translocations involving chromosome 12: report of a case and possible evidence for position effect. A girl with mental retardation, a convulsive disorder and clinical findings resembling cerebral palsy had an apparently balanced 12/18 translocation. Three additional cases reported in the literature had an abnormal phenotype associated with the positioning of segments from other autosomes adjacent to the band 12q21. Penotype abnormalities were not present in 14 individuals in which the translocation involved breaks at other sites on chromosome no. 12. These findings suggest the possibility of a position effect on chromosome segments translocated adjacent to the region 12q21."}
{"id": "PMID:1087857", "title": "12P trisomy: a syndrome?", "content": "A 2 2/3-year-old girl with 12p trisomy resulting from a maternal translocation is presented. Her clinical symptoms are strikingly similar to the other four cases known in the literature. A characteristic combination of symptoms for a 12p trisomy syndrome is delineated.", "contents": "12P trisomy: a syndrome? A 2 2/3-year-old girl with 12p trisomy resulting from a maternal translocation is presented. Her clinical symptoms are strikingly similar to the other four cases known in the literature. A characteristic combination of symptoms for a 12p trisomy syndrome is delineated."}
{"id": "PMID:1087858", "title": "Chemically defined media for growing anaerobic bacteria of the genus Veillonella.", "content": "A chemically defined medium for Veillonella parvula and V. alcalescens is described. Some nutritional aspects of the two strains used were examined: the optimum concentration of reducing agents, the requirements for amino acids, diamines, vitamins and other growth factors, and the conditions needed for well balanced nutrition. No specific requirements for single amino acids were observed. A combination of L-cysteine, DL-aspartic acid, L-glutamic acid, L-serine and L-tyrosine, promoted growth. In V. alcalescens, serine could substitute both arginine and tryptophan (or histidine). No growth was obtained with ammonium salts as the sole N source. Decarboxylation of L-ornithine, L-lysine and L-arginine was not demonstrated in the Veillonella parvula strain, which required putrescine or cadaverine for growth. Spermine, spermidine, L-lysine, L-ornithine and L-arginine, could not substitute putrescine in Veillonella parvula. Veillonella alcalescens, which does not require putrescine in the medium, was able to decarboxylate L-ornithine while forming putrescine.", "contents": "Chemically defined media for growing anaerobic bacteria of the genus Veillonella. A chemically defined medium for Veillonella parvula and V. alcalescens is described. Some nutritional aspects of the two strains used were examined: the optimum concentration of reducing agents, the requirements for amino acids, diamines, vitamins and other growth factors, and the conditions needed for well balanced nutrition. No specific requirements for single amino acids were observed. A combination of L-cysteine, DL-aspartic acid, L-glutamic acid, L-serine and L-tyrosine, promoted growth. In V. alcalescens, serine could substitute both arginine and tryptophan (or histidine). No growth was obtained with ammonium salts as the sole N source. Decarboxylation of L-ornithine, L-lysine and L-arginine was not demonstrated in the Veillonella parvula strain, which required putrescine or cadaverine for growth. Spermine, spermidine, L-lysine, L-ornithine and L-arginine, could not substitute putrescine in Veillonella parvula. Veillonella alcalescens, which does not require putrescine in the medium, was able to decarboxylate L-ornithine while forming putrescine."}
{"id": "PMID:1087859", "title": "Erwinia salicis: its metabolism and variability in vitro, and a method to demonstrate the pathogen in the host.", "content": "Morphological, biochemical and serological features of eleven Erwinia salicis isolates were examined. Three groups were demonstrated, one of which comprises the Dutch isolates. Serological techniques proved to be a valuable addition to conventional plating techniques for detecting the pathogen. Willow wood extract with 5% sucrose, 0.06% \"Lab Lemco\" broth and 1.5% agar appeared to be a suitable medium for the isolation of E. salicis, because of its selectivity.", "contents": "Erwinia salicis: its metabolism and variability in vitro, and a method to demonstrate the pathogen in the host. Morphological, biochemical and serological features of eleven Erwinia salicis isolates were examined. Three groups were demonstrated, one of which comprises the Dutch isolates. Serological techniques proved to be a valuable addition to conventional plating techniques for detecting the pathogen. Willow wood extract with 5% sucrose, 0.06% \"Lab Lemco\" broth and 1.5% agar appeared to be a suitable medium for the isolation of E. salicis, because of its selectivity."}
{"id": "PMID:1087860", "title": "Energy metabolism of some representatives of the Haemophilus group.", "content": "The purpose of this investigation was to characterize the carbohydrate catabolism and the constellation of the respiratory chain components of Haemophilus influenzae RAMC 18 Bensted, H. parainfluenzae 1 Fleming, H. parainfluenzae 429 Pittman and H. aegyptius 180a Pittman. These strains represent several physiological types with respect to respiratory quinones and glucose catabolism. On addition of glucose or lactate to the complex growth medium a remarkable increase in cell mass was observed. Depending on the growth rate, carbohydrate degradation varied with the strains examined so that at the end of the exponential growth phase only small amounts of the supplements could be demonstrated. All strains were found to possess functional enzymes of Embden-Meyerhof-Parnas-, Entner-Doudoroff-pathways, hexosemonophosphate shunt, tricarboxylic acid cycle and gluconeogenesis with an extremely high activity of malate dehydrogenase. The concentration of cytochromes varied according to culture conditions. The cytochromes a1, d, o and b + c were found to occur under aerobic conditions. In cells grown anaerobically in the presence of fumarate cytochromes a1 and d could not be demonstrated. Under aerobic conditions preparations of H. parainfluenzae 1 Fleming exhibited an alpha-maximum at 558 nm, whereas under anaerobic culture conditions with fumarate as terminal electron acceptor an alpha-maximum at 552 nm occurred, suggesting different roles of b and c type cytochromes in aerobic and anaerobic electron transport to fumarate, respectively.", "contents": "Energy metabolism of some representatives of the Haemophilus group. The purpose of this investigation was to characterize the carbohydrate catabolism and the constellation of the respiratory chain components of Haemophilus influenzae RAMC 18 Bensted, H. parainfluenzae 1 Fleming, H. parainfluenzae 429 Pittman and H. aegyptius 180a Pittman. These strains represent several physiological types with respect to respiratory quinones and glucose catabolism. On addition of glucose or lactate to the complex growth medium a remarkable increase in cell mass was observed. Depending on the growth rate, carbohydrate degradation varied with the strains examined so that at the end of the exponential growth phase only small amounts of the supplements could be demonstrated. All strains were found to possess functional enzymes of Embden-Meyerhof-Parnas-, Entner-Doudoroff-pathways, hexosemonophosphate shunt, tricarboxylic acid cycle and gluconeogenesis with an extremely high activity of malate dehydrogenase. The concentration of cytochromes varied according to culture conditions. The cytochromes a1, d, o and b + c were found to occur under aerobic conditions. In cells grown anaerobically in the presence of fumarate cytochromes a1 and d could not be demonstrated. Under aerobic conditions preparations of H. parainfluenzae 1 Fleming exhibited an alpha-maximum at 558 nm, whereas under anaerobic culture conditions with fumarate as terminal electron acceptor an alpha-maximum at 552 nm occurred, suggesting different roles of b and c type cytochromes in aerobic and anaerobic electron transport to fumarate, respectively."}
{"id": "PMID:1087861", "title": "Ultrastructure of the ascospores of some species of the Torulaspora group.", "content": "Development and germination of the ascospores in species of the Torulaspora group of yeasts have been described. Most species had warty spores which, in sections, showed a dark outer layer consisting of the outer unit membrane of the prospore wall and a layer underneath formed at an early stage of development of the spores. In mature spores the light inner layer of the wall was delimited at the outside by a thin dark layer. The warts often contained dark material. The ascospores of two Pichia and three Debaryomyces species were studied for comparison; they differed in sections from the Torulaspora spores. The taxonomic implications of the ultrastructural observations have been discussed.", "contents": "Ultrastructure of the ascospores of some species of the Torulaspora group. Development and germination of the ascospores in species of the Torulaspora group of yeasts have been described. Most species had warty spores which, in sections, showed a dark outer layer consisting of the outer unit membrane of the prospore wall and a layer underneath formed at an early stage of development of the spores. In mature spores the light inner layer of the wall was delimited at the outside by a thin dark layer. The warts often contained dark material. The ascospores of two Pichia and three Debaryomyces species were studied for comparison; they differed in sections from the Torulaspora spores. The taxonomic implications of the ultrastructural observations have been discussed."}
{"id": "PMID:1087862", "title": "Energetic aspects of the metabolism of reduced sulphur compounds in Thiobacillus dentrificans.", "content": "Yields of Thiobacillus dentrificans on different substrates were compared. The organism was grown in a chemostat at a dilution rate of 0.03 h-1. From the difference in the cell yields with (1) oxygen (6.40 g carbon per mole thiosulphate) and (2) nitrate (4.51 g carbon per mole thiosulphate) as an electron acceptor the experimental value for Y ATP was estimated to be 1.75. The efficiency of the biosynthetic system would be 42% if 1 ATP should be needed in reversed electron transport, and 57% if this was 2 ATP per electron pair. It could be calculated that during anaerobic oxidation of thiosulphate with nitrate 1.41 or 1.16 ATP per 2 electrons are generated if 1 or 2 ATP respectively per thiosulphate is formed in substrate-level phosphorylation. For aerobic oxidation these figures are 2.40 and 2.16, respectively.", "contents": "Energetic aspects of the metabolism of reduced sulphur compounds in Thiobacillus dentrificans. Yields of Thiobacillus dentrificans on different substrates were compared. The organism was grown in a chemostat at a dilution rate of 0.03 h-1. From the difference in the cell yields with (1) oxygen (6.40 g carbon per mole thiosulphate) and (2) nitrate (4.51 g carbon per mole thiosulphate) as an electron acceptor the experimental value for Y ATP was estimated to be 1.75. The efficiency of the biosynthetic system would be 42% if 1 ATP should be needed in reversed electron transport, and 57% if this was 2 ATP per electron pair. It could be calculated that during anaerobic oxidation of thiosulphate with nitrate 1.41 or 1.16 ATP per 2 electrons are generated if 1 or 2 ATP respectively per thiosulphate is formed in substrate-level phosphorylation. For aerobic oxidation these figures are 2.40 and 2.16, respectively."}
{"id": "PMID:1087863", "title": "Hereditary respiration deficiency in Saccharomycodes ludwigii.", "content": "Saccharomycodes ludwigii, supposed to be \"petite-negative,\" gave rise to respiration-deficient mutants when acriflavine and ultraviolet irradiation, respectively, were applied to this yeast, strain IFO 1194. The frequency of such mutants was very low as compared with that in Saccharomyces cervisiae and other \"petite-positive\" yeasts. Cytochrome composition was characterized by spectrophotometry at the temperature of liquid nitrogen. The respiratory mutants examined contained cytochrome c unaltered in quality and quantity. Cytochrome b was often present only in small amounts though never absent, while cytochrome a + a3 was either present or absent. The respiratory mutants could form zygotes after conjugation with a wild-type culture of opposite mating type (alpha vs. a). The hybridization and segregation analysis of spore tetrads showed the inheritance of respiratory mutant character to be either Mendelian or non-Mendelian and similar to that of pet (nuclear) and rho- (cytoplasmic) mutants, respectively, in Saccharomyces cerevisiae.", "contents": "Hereditary respiration deficiency in Saccharomycodes ludwigii. Saccharomycodes ludwigii, supposed to be \"petite-negative,\" gave rise to respiration-deficient mutants when acriflavine and ultraviolet irradiation, respectively, were applied to this yeast, strain IFO 1194. The frequency of such mutants was very low as compared with that in Saccharomyces cervisiae and other \"petite-positive\" yeasts. Cytochrome composition was characterized by spectrophotometry at the temperature of liquid nitrogen. The respiratory mutants examined contained cytochrome c unaltered in quality and quantity. Cytochrome b was often present only in small amounts though never absent, while cytochrome a + a3 was either present or absent. The respiratory mutants could form zygotes after conjugation with a wild-type culture of opposite mating type (alpha vs. a). The hybridization and segregation analysis of spore tetrads showed the inheritance of respiratory mutant character to be either Mendelian or non-Mendelian and similar to that of pet (nuclear) and rho- (cytoplasmic) mutants, respectively, in Saccharomyces cerevisiae."}
{"id": "PMID:1087864", "title": "Homothallism in wine yeasts.", "content": "In an attempt to improve by hybridisation strains of pure-culture wine yeasts it could be shown, that of the seven strains used five are homothallic. Evidence is presented suggesting that the remainder are also homothallic.", "contents": "Homothallism in wine yeasts. In an attempt to improve by hybridisation strains of pure-culture wine yeasts it could be shown, that of the seven strains used five are homothallic. Evidence is presented suggesting that the remainder are also homothallic."}
{"id": "PMID:1087865", "title": "Comparison between the yeast flora of Middle Eastern and Western European soft drinks.", "content": "Samples of a carbonated orange drink, raw materials, and intermediate products originating from 6 Iraqi bottling plants were examined. 69 drinks, 4 flavoured syrups and 19 simple syrups contained yeasts, whereas all samples from one plant and all samples of beverage base were free from viable yeasts. From the orange drink 2 species were isolated viz. Saccharomyces montanus and Torulopsis stellata. The following species were present in simple syrup: Hansenula anomala, Sacch. bisporus var. mellis, T. candida and T. stellata. Sacch. bisporus var. mellis was also isolated from favoured syrup. Representative strains were submitted to routine growth tests at reduced oxygen tension, at reduced water activity and on solid soft-drink media containing various amounts of anti-microbially active benzoic acid at pH 3.0. The results are discussed and compared to those obtained in European soft drinks.", "contents": "Comparison between the yeast flora of Middle Eastern and Western European soft drinks. Samples of a carbonated orange drink, raw materials, and intermediate products originating from 6 Iraqi bottling plants were examined. 69 drinks, 4 flavoured syrups and 19 simple syrups contained yeasts, whereas all samples from one plant and all samples of beverage base were free from viable yeasts. From the orange drink 2 species were isolated viz. Saccharomyces montanus and Torulopsis stellata. The following species were present in simple syrup: Hansenula anomala, Sacch. bisporus var. mellis, T. candida and T. stellata. Sacch. bisporus var. mellis was also isolated from favoured syrup. Representative strains were submitted to routine growth tests at reduced oxygen tension, at reduced water activity and on solid soft-drink media containing various amounts of anti-microbially active benzoic acid at pH 3.0. The results are discussed and compared to those obtained in European soft drinks."}
{"id": "PMID:1087866", "title": "Taxonomical examination and characterization of a methanol-utilizing yeast.", "content": "The morphological, cultural, and physiological characteristics are described of a yeast, LI70, which uses methanol as its source of energy and carbon; these characteristics have made it possible to identify the strain as Candida boidinii Ramirez. The identification was confirmed by a DNA-DNA genetic homology of 99.43% with the type strain of C. boidinii. Strain LI70 is not pathogenic.", "contents": "Taxonomical examination and characterization of a methanol-utilizing yeast. The morphological, cultural, and physiological characteristics are described of a yeast, LI70, which uses methanol as its source of energy and carbon; these characteristics have made it possible to identify the strain as Candida boidinii Ramirez. The identification was confirmed by a DNA-DNA genetic homology of 99.43% with the type strain of C. boidinii. Strain LI70 is not pathogenic."}
{"id": "PMID:1087867", "title": "Rickets associated with anticonvulsant therapy in children with tuberous sclerosis.", "content": "Five children with tuberous sclerosis and anti-convulsant-associated rickets have been seen in a general paediatric clinic over the last 5 years. It is suggested that vitamin D prophylaxis is particularly indicated in patients with tuberous sclerosis taking anticonvulsant medication.", "contents": "Rickets associated with anticonvulsant therapy in children with tuberous sclerosis. Five children with tuberous sclerosis and anti-convulsant-associated rickets have been seen in a general paediatric clinic over the last 5 years. It is suggested that vitamin D prophylaxis is particularly indicated in patients with tuberous sclerosis taking anticonvulsant medication."}
{"id": "PMID:1087868", "title": "[Microbiological drawings in pneumology (author's transl)].", "content": "Microbiological examinations of the sputum for clinical purposes is often not satisfactory or useless. Suitability, chances of oropharyngeal contamination, hazards for the patients and clinical indications of the various drawing methods for tracheobronchial secretions and exudates are different. Transcutaneous tracheal aspiration is easy, reliable and safe.", "contents": "[Microbiological drawings in pneumology (author's transl)]. Microbiological examinations of the sputum for clinical purposes is often not satisfactory or useless. Suitability, chances of oropharyngeal contamination, hazards for the patients and clinical indications of the various drawing methods for tracheobronchial secretions and exudates are different. Transcutaneous tracheal aspiration is easy, reliable and safe."}
{"id": "PMID:1087872", "title": "Effect of cordycepin on the replication of western equine encephalitis virus.", "content": "Cordycepin (3'-deoxyadenosine) inhibited viral RNA synthesis in the replication of western equine encephalitis virus, thereby causing a reduction of virus production. The rate of inhibition of viral RNA synthesis was dependent on drug concentration and the period of treatment with the drug. These results suggest that the virus RNA synthesizing system is sensitive to the drug.", "contents": "Effect of cordycepin on the replication of western equine encephalitis virus. Cordycepin (3'-deoxyadenosine) inhibited viral RNA synthesis in the replication of western equine encephalitis virus, thereby causing a reduction of virus production. The rate of inhibition of viral RNA synthesis was dependent on drug concentration and the period of treatment with the drug. These results suggest that the virus RNA synthesizing system is sensitive to the drug."}
{"id": "PMID:1087869", "title": "[Parasites of the respiratory system: research and significance (author's transl)].", "content": "A review is made of the methods of diagnosing both autochthonous and exotic protozoal and helminthic diseases of the respiratory system. Referring to protozooses, recent findings on respiratory pathology due to amoebae of the genus Acanthamoeba are commented, and modern methods are discussed of checking for Pneumocystis carinii in the patient, not just on autopsy material. Referring to helminthiases, in addition to pulmonary echinococcosis which is of prevalent interest in Italy, attention is also given to the pathology of migrant larvae of nematodes. Finally, the role of some microscopic mites in the pathogenesis of respiratory allergic disease from house dust is discussed.", "contents": "[Parasites of the respiratory system: research and significance (author's transl)]. A review is made of the methods of diagnosing both autochthonous and exotic protozoal and helminthic diseases of the respiratory system. Referring to protozooses, recent findings on respiratory pathology due to amoebae of the genus Acanthamoeba are commented, and modern methods are discussed of checking for Pneumocystis carinii in the patient, not just on autopsy material. Referring to helminthiases, in addition to pulmonary echinococcosis which is of prevalent interest in Italy, attention is also given to the pathology of migrant larvae of nematodes. Finally, the role of some microscopic mites in the pathogenesis of respiratory allergic disease from house dust is discussed."}
{"id": "PMID:1087873", "title": "[T- and B-lymphocytes. Distribution in the body, their functional and morphological characteristics and significance].", "content": "In a survey of the literature special attention is given to the history of development of concepts concerning the existence in the immunocompetent system of the lymphoid tissue of higher vertebrals and man of two independent to some extent populations of lymphocytes: one dependent on the thymus gland and therefore called T-lymphocytes population, implementing cellular reactions of immunity; and the other, similar one, dependent on Fabricius' bursa in birds and called B-lymphocytes, producing antibodies circulaing in the blood. Functional-morphological characteristics of T-and B-lymphocytes, their distribution in the peripheral organs of the lymphoid system and in the blood are given. The role of T-and B-lymphocytes in rejection of allotransplants and in the reaction of a transplant against the host development of reactions of hypersensitivity of the delayed and immediate types is discussed. The need for a further study of participation of T-and B-lymphocytes in pathogenesis of allergic, autoimmune and numerous other diseases that are accompanied by immune disorders, is emphasized.", "contents": "[T- and B-lymphocytes. Distribution in the body, their functional and morphological characteristics and significance]. In a survey of the literature special attention is given to the history of development of concepts concerning the existence in the immunocompetent system of the lymphoid tissue of higher vertebrals and man of two independent to some extent populations of lymphocytes: one dependent on the thymus gland and therefore called T-lymphocytes population, implementing cellular reactions of immunity; and the other, similar one, dependent on Fabricius' bursa in birds and called B-lymphocytes, producing antibodies circulaing in the blood. Functional-morphological characteristics of T-and B-lymphocytes, their distribution in the peripheral organs of the lymphoid system and in the blood are given. The role of T-and B-lymphocytes in rejection of allotransplants and in the reaction of a transplant against the host development of reactions of hypersensitivity of the delayed and immediate types is discussed. The need for a further study of participation of T-and B-lymphocytes in pathogenesis of allergic, autoimmune and numerous other diseases that are accompanied by immune disorders, is emphasized."}
{"id": "PMID:1087870", "title": "[Introduction to a study of the hydrosoluble proteins of the sputum (author's transl)].", "content": "Synthesis of a study of the proteins outline of the bronchial secretion with the qualitative and quantitative determination of six protein fractions (albumin, Alfa-antitrypsin, acid Alfa-glycoprotein, haptoglobin, transferrin); comparation of the concentration of these fractions of the expectoration with those of the serum of the patient himself.", "contents": "[Introduction to a study of the hydrosoluble proteins of the sputum (author's transl)]. Synthesis of a study of the proteins outline of the bronchial secretion with the qualitative and quantitative determination of six protein fractions (albumin, Alfa-antitrypsin, acid Alfa-glycoprotein, haptoglobin, transferrin); comparation of the concentration of these fractions of the expectoration with those of the serum of the patient himself."}
{"id": "PMID:1087876", "title": "Plasma membrane of a murine T cell lymphoma: surface labelling, membrane isolation, separation of membrane proteins and distribution of surface label amongst these proteins.", "content": "Two established techniques for analysis of plasma membranes, namely, lactoperoxidase catalyzed surface radioiodination of intact cells and bulk membrane isolation following disruption of cells by shear forces, were applied in studies of membrane proteins of continuously cultured cells of the monoclonal T lymphoma line WEHI-22. It was found that macromolecular 125I-iodide incorporated into plasma membrane proteins of intact cells was at least as good a marker for the plasma as was the commonly used enzyme 5'-nucleotidase. T lymphoma plasma membrane proteins were complex when analysed by polyacrylamide gel electrophoresis in sodium dodecylsulphate-containing buffers and more than thirty distinct components were resolved. More than fifteen of the components observed on a mass basis were also labelled with 125I-iodide. Certain bands, however, exhibited a degree of label disproportionate to their staining properties with Coomassie Blue. This was interpreted in terms of their accessibility to the solvent in the intact cells.", "contents": "Plasma membrane of a murine T cell lymphoma: surface labelling, membrane isolation, separation of membrane proteins and distribution of surface label amongst these proteins. Two established techniques for analysis of plasma membranes, namely, lactoperoxidase catalyzed surface radioiodination of intact cells and bulk membrane isolation following disruption of cells by shear forces, were applied in studies of membrane proteins of continuously cultured cells of the monoclonal T lymphoma line WEHI-22. It was found that macromolecular 125I-iodide incorporated into plasma membrane proteins of intact cells was at least as good a marker for the plasma as was the commonly used enzyme 5'-nucleotidase. T lymphoma plasma membrane proteins were complex when analysed by polyacrylamide gel electrophoresis in sodium dodecylsulphate-containing buffers and more than thirty distinct components were resolved. More than fifteen of the components observed on a mass basis were also labelled with 125I-iodide. Certain bands, however, exhibited a degree of label disproportionate to their staining properties with Coomassie Blue. This was interpreted in terms of their accessibility to the solvent in the intact cells."}
{"id": "PMID:1087877", "title": "Pulmonary elastic recoil and diffusing capacity in subjects with intermediate concentrations of alpha-1 antitrypsin.", "content": "Serum from 224 males investigated in a prospective study of respiratory disorders in a recently established asbestos industry has been estimated for alpha-1 antitrypsin (AAT) concentration by single radial immunodiffusion. Mean AAT concentration was 213-4 mg/dl). No subject with a markedly reduced level was found. A comprehensive range of lung function tests sensitive to changes anticipated in emphysema at a preclinical stage, included measurements of diffusing capacity and pulmonary elastic recoil. The 26 subjects with AAT concentration less than 150 mg/dl, were regarded as likely to comprise a majority of genotypes MZ and SS, and the 19 subjects with AAT concentration greater than 300 mg/dl were considered to comprise mainly MM. No difference in pulmonary function could be demonstrated between these two groups. Evidence of diminished pulmonary elastic recoil was found in nine smoking subjects whose AAT concentrations were normal and also in one young non-smoking subject with moderately severe asthma, whose AAT concentration was 140 mg/dl. It is concluded that in a male working population, evidence of diminished pulmonary elastic recoil is not a function of AAT concentration.", "contents": "Pulmonary elastic recoil and diffusing capacity in subjects with intermediate concentrations of alpha-1 antitrypsin. Serum from 224 males investigated in a prospective study of respiratory disorders in a recently established asbestos industry has been estimated for alpha-1 antitrypsin (AAT) concentration by single radial immunodiffusion. Mean AAT concentration was 213-4 mg/dl). No subject with a markedly reduced level was found. A comprehensive range of lung function tests sensitive to changes anticipated in emphysema at a preclinical stage, included measurements of diffusing capacity and pulmonary elastic recoil. The 26 subjects with AAT concentration less than 150 mg/dl, were regarded as likely to comprise a majority of genotypes MZ and SS, and the 19 subjects with AAT concentration greater than 300 mg/dl were considered to comprise mainly MM. No difference in pulmonary function could be demonstrated between these two groups. Evidence of diminished pulmonary elastic recoil was found in nine smoking subjects whose AAT concentrations were normal and also in one young non-smoking subject with moderately severe asthma, whose AAT concentration was 140 mg/dl. It is concluded that in a male working population, evidence of diminished pulmonary elastic recoil is not a function of AAT concentration."}
{"id": "PMID:1087878", "title": "Gastric ulcer: natural history and treatment.", "content": "The fragmentary knowledge of gastric ulcer aetiology is contrasted with the developing repertoire of short term drug treatments. Reasons are also advanced for believing that gastric ulcer may be a disease of diminishing importance and that this is due to environmental changes rather than to the introduction of effective treatments.", "contents": "Gastric ulcer: natural history and treatment. The fragmentary knowledge of gastric ulcer aetiology is contrasted with the developing repertoire of short term drug treatments. Reasons are also advanced for believing that gastric ulcer may be a disease of diminishing importance and that this is due to environmental changes rather than to the introduction of effective treatments."}
{"id": "PMID:1087879", "title": "Gastric damage by drugs and the role of the mucosal barrier.", "content": "There are six drugs usually implicated in peptic ulceration and these are adrenal corticosteroids, aspirin, phenylbutazone, indomethacin, ethanol and caffeine.  The types of data upon which these conclusions rest follows three lines of evidence.  First, the production of ulcers in experimental animals; all the drugs mentioned above can produce experimental ulcers.  Second, the cause and effect relationship in man, i.e. epidemiological evidence; the epidemiological evidence is very weak except for aspirin.  Third, a mechanism suspected of participating in the pathogenesis; the pathogenesis of drug ulceration is not fully understood but aspirin may be the only one with a body of data to support its ulcerogenic effect.  The pathogenesis of peptic ulcer is usually considered in terms of the equation, acid-pepsin versus mucosal resistance.  Caffeine is a moderately strong stimulus of acid secretion but corticosteroids, phenylbutazone, ethanol and indomethacin are very weak stimulants or have no effect.  Aspirin decreases acid secretion...", "contents": "Gastric damage by drugs and the role of the mucosal barrier. There are six drugs usually implicated in peptic ulceration and these are adrenal corticosteroids, aspirin, phenylbutazone, indomethacin, ethanol and caffeine.  The types of data upon which these conclusions rest follows three lines of evidence.  First, the production of ulcers in experimental animals; all the drugs mentioned above can produce experimental ulcers.  Second, the cause and effect relationship in man, i.e. epidemiological evidence; the epidemiological evidence is very weak except for aspirin.  Third, a mechanism suspected of participating in the pathogenesis; the pathogenesis of drug ulceration is not fully understood but aspirin may be the only one with a body of data to support its ulcerogenic effect.  The pathogenesis of peptic ulcer is usually considered in terms of the equation, acid-pepsin versus mucosal resistance.  Caffeine is a moderately strong stimulus of acid secretion but corticosteroids, phenylbutazone, ethanol and indomethacin are very weak stimulants or have no effect.  Aspirin decreases acid secretion..."}
{"id": "PMID:1087883", "title": "Effects of an antitumor multipeptide complex on immunocompetent and antigen-responsive cells.", "content": "The investigations, performed to study the possible mechanism of action of PTC, of its single constituents and of meta-levo-sarcolysin (m-L-SL) on the human immunocompetent system, are reported. The study entailed the evaluation of the effect of PTC, of its peptides and of m-L-SL on the various lymphocyte classes, employing all the more sophisticated immunological techniques such as: a) lymphocyte cultures stimulated with the various mitogens, (T and B lymphocytes); b) E rosettes (T lymphocytes); c) EA rosettes (lymphocytes with Fc receptors for IgG); d) EAC rosettes (lymphocytes with the C3 receptors); e) autoradiography and intracytoplasmic immunofluorescence (in vitro immunoglobulin neosynthesis); f) CdL (Complement-dependent Lymphocytotoxicity); g) LALI (Lymphocyte Antibody Lymphocytolytic Interaction); h) CML (antibody independent cytotoxicity i.e. Cell-Mediated Lympholysis). The evidence points out that the PTC effect on T and B lymphocytes is not comparable to that of m-L-SL which does not discriminate between the two lymphocyte populations while PTC, besides showing a scarce immunodepressive effect, preferentially affects B lymphocytes rather than T lymphocytes as shown also by the E rosette pattern. Significantly different appears also the influence of PTC, as compared with that of m-L-SL on the lymphocytes with Fc receptors for IgG (EA rosettes) and with receptors for the third component of complement (EAC rosettes) as well as on the cytotoxicity test and on the antibody dependent (LALI) and complement dependent (CdL) lytic effect. The data gleaned from this investigation allow evaluating both the effects of PTC on the immunocompetent system and the difference of its action from that of m-L-SL.", "contents": "Effects of an antitumor multipeptide complex on immunocompetent and antigen-responsive cells. The investigations, performed to study the possible mechanism of action of PTC, of its single constituents and of meta-levo-sarcolysin (m-L-SL) on the human immunocompetent system, are reported. The study entailed the evaluation of the effect of PTC, of its peptides and of m-L-SL on the various lymphocyte classes, employing all the more sophisticated immunological techniques such as: a) lymphocyte cultures stimulated with the various mitogens, (T and B lymphocytes); b) E rosettes (T lymphocytes); c) EA rosettes (lymphocytes with Fc receptors for IgG); d) EAC rosettes (lymphocytes with the C3 receptors); e) autoradiography and intracytoplasmic immunofluorescence (in vitro immunoglobulin neosynthesis); f) CdL (Complement-dependent Lymphocytotoxicity); g) LALI (Lymphocyte Antibody Lymphocytolytic Interaction); h) CML (antibody independent cytotoxicity i.e. Cell-Mediated Lympholysis). The evidence points out that the PTC effect on T and B lymphocytes is not comparable to that of m-L-SL which does not discriminate between the two lymphocyte populations while PTC, besides showing a scarce immunodepressive effect, preferentially affects B lymphocytes rather than T lymphocytes as shown also by the E rosette pattern. Significantly different appears also the influence of PTC, as compared with that of m-L-SL on the lymphocytes with Fc receptors for IgG (EA rosettes) and with receptors for the third component of complement (EAC rosettes) as well as on the cytotoxicity test and on the antibody dependent (LALI) and complement dependent (CdL) lytic effect. The data gleaned from this investigation allow evaluating both the effects of PTC on the immunocompetent system and the difference of its action from that of m-L-SL."}
{"id": "PMID:1087884", "title": "Complement receptors (C3b, C4b/C3d) unbalance on CLL lymphocytes.", "content": "A study of lymphocytes bearing C3b, C4b and C3d complement receptor (CRL) was performed on human peripheral blood from 16 healthy donors and 12 patients affected with Chronic Lymphatic Leukemia (CLL). In the latter group a clear rise of C3dCRL was demonstrated, when compared with immunoadherence receptors bearing lymphocytes. However, when compared with controls, also these latter were slightly augmented. Furthermore in CLL under treatment CRL populations showed the same profile as CLL: so it was suggested that the treatment reduced not selectively all the three types of CRL, within the population of sIg bearing lymphocytes. Here we discuss the hypothesis that, in CLL, the proliferating lymphocytes population is chiefly sIg+, C3d+/C3b-, C4b-, but also sIg+, C3d+/C3b+, C4b+.", "contents": "Complement receptors (C3b, C4b/C3d) unbalance on CLL lymphocytes. A study of lymphocytes bearing C3b, C4b and C3d complement receptor (CRL) was performed on human peripheral blood from 16 healthy donors and 12 patients affected with Chronic Lymphatic Leukemia (CLL). In the latter group a clear rise of C3dCRL was demonstrated, when compared with immunoadherence receptors bearing lymphocytes. However, when compared with controls, also these latter were slightly augmented. Furthermore in CLL under treatment CRL populations showed the same profile as CLL: so it was suggested that the treatment reduced not selectively all the three types of CRL, within the population of sIg bearing lymphocytes. Here we discuss the hypothesis that, in CLL, the proliferating lymphocytes population is chiefly sIg+, C3d+/C3b-, C4b-, but also sIg+, C3d+/C3b+, C4b+."}
{"id": "PMID:1087885", "title": "[Comparison between anti-lymphocyte antibodies in systemic lupus erythematosus and in Hodgkin's disease].", "content": "Anti-lymphocyte antibodies of cold-reactive IgM-type in Systemic Lupus Erythematosus (SLE) and IgG-type in Hodgkin's disease (HD) by means of immunofluorescent technique have been demonstrated. The contemporaneous attachment of SLE and HD sera on selected peripheral lymphocytes demonstrate that the target cell is T-lymphocyte and that the two kinds of antibodies coat the same cell. Co-capping experiments have shown that the phenomenon has the same behaviour as regard to SLE and HD antibody, indicating that the antigen is the same. Hypothesis about the meaning of these antibodyies have been made.", "contents": "[Comparison between anti-lymphocyte antibodies in systemic lupus erythematosus and in Hodgkin's disease]. Anti-lymphocyte antibodies of cold-reactive IgM-type in Systemic Lupus Erythematosus (SLE) and IgG-type in Hodgkin's disease (HD) by means of immunofluorescent technique have been demonstrated. The contemporaneous attachment of SLE and HD sera on selected peripheral lymphocytes demonstrate that the target cell is T-lymphocyte and that the two kinds of antibodies coat the same cell. Co-capping experiments have shown that the phenomenon has the same behaviour as regard to SLE and HD antibody, indicating that the antigen is the same. Hypothesis about the meaning of these antibodyies have been made."}
{"id": "PMID:1087887", "title": "[Hematopoietic stem cells and antiblastic drugs. II. Variations in the colony forming cells in vivo after administration of peptichemio].", "content": "Peptichemio, administered in a single LD50, dose, induces a significant reduction of mice bone marrow and spleen CFUs. The lesive action appears to be similar to that observed after a single dose of various antineoplastic agents. The effects of increasing doses of Peptichemio are also reported.", "contents": "[Hematopoietic stem cells and antiblastic drugs. II. Variations in the colony forming cells in vivo after administration of peptichemio]. Peptichemio, administered in a single LD50, dose, induces a significant reduction of mice bone marrow and spleen CFUs. The lesive action appears to be similar to that observed after a single dose of various antineoplastic agents. The effects of increasing doses of Peptichemio are also reported."}
{"id": "PMID:1087888", "title": "Enrichment of B lymphocytes from human peripheral blood by sedimentation of E rosette stabilized with dextran.", "content": "A simple method for the enrichment of B lymphocytes population from human peripheral blood by isolated of lymphocytes responsible for E rosette in presence of dextran is presented. Qualitative and quantitative results regarding the yield, the viability and the purity of the enriched population are discussed.", "contents": "Enrichment of B lymphocytes from human peripheral blood by sedimentation of E rosette stabilized with dextran. A simple method for the enrichment of B lymphocytes population from human peripheral blood by isolated of lymphocytes responsible for E rosette in presence of dextran is presented. Qualitative and quantitative results regarding the yield, the viability and the purity of the enriched population are discussed."}
{"id": "PMID:1087889", "title": "Peripheral blood T lymphocyte subpopulations in newborns.", "content": "Monocyte-depleted lymphocyte suspensions from the peripheral blood of 36 healthy newborns and 23 adults were compared as to percent of cells forming rosettes with sheep erythrocytes (T lymphocytes) and proliferative responses after in vitro culture with various doses of Phytohemagglutinin, Concanavalin A and Pokeweed mitogen. No significant difference was found between the proportions of T lymphocytes of newborns and adults. Mitogen responsiveness of the newborn lymphocytes showed dramatic between-subject variability completely unrelated to the proportion of T lymphocytes. It is suggested that during neonatal life there is massive variable recirculation of T lymphocytes subsets (and/or stages of maturation) characterized by differential degrees of responsiveness to the mitogens tested.", "contents": "Peripheral blood T lymphocyte subpopulations in newborns. Monocyte-depleted lymphocyte suspensions from the peripheral blood of 36 healthy newborns and 23 adults were compared as to percent of cells forming rosettes with sheep erythrocytes (T lymphocytes) and proliferative responses after in vitro culture with various doses of Phytohemagglutinin, Concanavalin A and Pokeweed mitogen. No significant difference was found between the proportions of T lymphocytes of newborns and adults. Mitogen responsiveness of the newborn lymphocytes showed dramatic between-subject variability completely unrelated to the proportion of T lymphocytes. It is suggested that during neonatal life there is massive variable recirculation of T lymphocytes subsets (and/or stages of maturation) characterized by differential degrees of responsiveness to the mitogens tested."}
{"id": "PMID:1087890", "title": "[Effect of L-DOPA on repair of neurogenic dystrophic damage to the gastric mucosa of rats].", "content": "Neurogenic dystrophy of the gastric mucosa was induced by a 3-hour immobilization and electrostimulation. By the end of the stimulation there developed in the gastric mucosa hemorrhagic erosions which persisted for two days after the stimulation. According to the data of macro- and microscopic studies injections of 1-DOPA to rats in a dose of 10 mg/kg of body weight for a period of two days after the cessation of stimulation accelerated the healing of hemorrhagic erosions of the gastric mucosa.", "contents": "[Effect of L-DOPA on repair of neurogenic dystrophic damage to the gastric mucosa of rats]. Neurogenic dystrophy of the gastric mucosa was induced by a 3-hour immobilization and electrostimulation. By the end of the stimulation there developed in the gastric mucosa hemorrhagic erosions which persisted for two days after the stimulation. According to the data of macro- and microscopic studies injections of 1-DOPA to rats in a dose of 10 mg/kg of body weight for a period of two days after the cessation of stimulation accelerated the healing of hemorrhagic erosions of the gastric mucosa."}
{"id": "PMID:1087891", "title": "Action of isoprenaline on the mechanical properties of lungs and airways in healthy people and patients with obstructive lung diseases.", "content": "Lung volumes, maximal expiratory flows, pulmonary resistance and static recoil pressure-volume curves were measured before and after inhalation of isoprenaline in four groups of subjects: heqlthy patients with mild and severe chronic bronchitis and asthmatics. The observed changes were qualitatively similar in the four groups. Whereas total lung capacity and dynamic compliance were not influenced, there was an increase in vital capacity (not in healthy subjects and mild bronchitics), in FEV1, in maximal expiratory flows and a decrease in pulmonary resistance. The static recoil pressure-volume relationship was shifted to the left over its ascending part. The effect of isoprenaline tended to be larger in patients. Among the latter, the increase of vital capacity was especially pronounced  in the severe bronchitics, whereas the maximal flows increased most in asthmatics. We suggest that the influence of isoprenaline on maximal flows results from a bronchodilation, associated with an increased collapsibility of the central intrathoracic airways, whereas the influence of VC might reflect a delayed onset of small airway closure.", "contents": "Action of isoprenaline on the mechanical properties of lungs and airways in healthy people and patients with obstructive lung diseases. Lung volumes, maximal expiratory flows, pulmonary resistance and static recoil pressure-volume curves were measured before and after inhalation of isoprenaline in four groups of subjects: heqlthy patients with mild and severe chronic bronchitis and asthmatics. The observed changes were qualitatively similar in the four groups. Whereas total lung capacity and dynamic compliance were not influenced, there was an increase in vital capacity (not in healthy subjects and mild bronchitics), in FEV1, in maximal expiratory flows and a decrease in pulmonary resistance. The static recoil pressure-volume relationship was shifted to the left over its ascending part. The effect of isoprenaline tended to be larger in patients. Among the latter, the increase of vital capacity was especially pronounced  in the severe bronchitics, whereas the maximal flows increased most in asthmatics. We suggest that the influence of isoprenaline on maximal flows results from a bronchodilation, associated with an increased collapsibility of the central intrathoracic airways, whereas the influence of VC might reflect a delayed onset of small airway closure."}
{"id": "PMID:1087898", "title": "The renal handling of biologically active peptides.", "content": "With the use of the protease inhibitor from bovine organs--Trasylol-- as a model, we studied the pinocytic transport of peptides in the kidney. Rats were injected with the 125I-labeled peptide and killed at different times thereafter. Kidney homogenates were subfractionated by differential and sucrose gradient centrifugation. Radioactivity was measured in the fractions in order to study the time-dependent fixation of the peptide to different cell organelles. With short survival periods, the protease inhibitor is recovered in the brush-border fraction, with longer periods, a shift towards the lysosome fraction takes place. Thus, the renal transport of the protease inhibitor consists of three steps: binding to the brush border, incorporation in micropinocytic vesicles and transport in phagolysosomes.", "contents": "The renal handling of biologically active peptides. With the use of the protease inhibitor from bovine organs--Trasylol-- as a model, we studied the pinocytic transport of peptides in the kidney. Rats were injected with the 125I-labeled peptide and killed at different times thereafter. Kidney homogenates were subfractionated by differential and sucrose gradient centrifugation. Radioactivity was measured in the fractions in order to study the time-dependent fixation of the peptide to different cell organelles. With short survival periods, the protease inhibitor is recovered in the brush-border fraction, with longer periods, a shift towards the lysosome fraction takes place. Thus, the renal transport of the protease inhibitor consists of three steps: binding to the brush border, incorporation in micropinocytic vesicles and transport in phagolysosomes."}
{"id": "PMID:1087901", "title": "Sunscreen dermatitis due to glyceryl PABA: significance of cross-reactions to this PABA Ester.", "content": "Glyceryl PABA (Escalol 106), present in many popular sunscreens, is a common sensitizer which invariably cross-reacts with benzocaine; it is present in over 400 over-the-counter products. Glyceryl PABA does not appear to cross-react with the sunscreening agents amyl (Escalol 506) and octyl (Escalol 507) dimethyl PABA. Glyceryl PABA, may, on occasion, cross-react with para-aminobenzoic acid (PABA), paraphenylenediamine, aniline, and sulfa drugs. Ironically, the sunscreening agent glyceryl PABA may, itself, act as a photosensitizing agent.", "contents": "Sunscreen dermatitis due to glyceryl PABA: significance of cross-reactions to this PABA Ester. Glyceryl PABA (Escalol 106), present in many popular sunscreens, is a common sensitizer which invariably cross-reacts with benzocaine; it is present in over 400 over-the-counter products. Glyceryl PABA does not appear to cross-react with the sunscreening agents amyl (Escalol 506) and octyl (Escalol 507) dimethyl PABA. Glyceryl PABA, may, on occasion, cross-react with para-aminobenzoic acid (PABA), paraphenylenediamine, aniline, and sulfa drugs. Ironically, the sunscreening agent glyceryl PABA may, itself, act as a photosensitizing agent."}
{"id": "PMID:1087902", "title": "Lentiginosis profusa. III: Aesthetic and sanguineous aspects.", "content": "Lentiginosis profusa syndrome and progressive cardiomyopathic lentiginosis are preferable terms to \"leopard syndrome\". Considerable cosmetic improvement followed facial dermabrasion and light electrodesiccation of lentigines on the exposed surfaces. Hematologic evaluation did not reveal hematopoietic defect in the patient studied.", "contents": "Lentiginosis profusa. III: Aesthetic and sanguineous aspects. Lentiginosis profusa syndrome and progressive cardiomyopathic lentiginosis are preferable terms to \"leopard syndrome\". Considerable cosmetic improvement followed facial dermabrasion and light electrodesiccation of lentigines on the exposed surfaces. Hematologic evaluation did not reveal hematopoietic defect in the patient studied."}
{"id": "PMID:1087904", "title": "Age, immunity and the skin.", "content": "With advancing age there is a decrease in T-lymphocyte functions leading to defective cell-mediated immune responses and decreased levels of immunoglobulin G, which is produced by B cells under the influence of certain T lymphocytes. Decreased resistance to viruses as a result of the depression of T-cell function may lead to some clinical viral infections which trigger autoimmune diseases. The diminished T cell's immune response and its inability to eliminate ultraviolet-light-damaged epidermal cells may be in part responsible for the increased incidence of skin malignancies in elderly patients.", "contents": "Age, immunity and the skin. With advancing age there is a decrease in T-lymphocyte functions leading to defective cell-mediated immune responses and decreased levels of immunoglobulin G, which is produced by B cells under the influence of certain T lymphocytes. Decreased resistance to viruses as a result of the depression of T-cell function may lead to some clinical viral infections which trigger autoimmune diseases. The diminished T cell's immune response and its inability to eliminate ultraviolet-light-damaged epidermal cells may be in part responsible for the increased incidence of skin malignancies in elderly patients."}
{"id": "PMID:1087905", "title": "Quantitative scintigraphic display of myocardial blood flow. Technique and clinical evaluation.", "content": "Immediately after coronarography 10 mCi 133Xenon were injected into the left or right coronary artery. A scintillation camera was placed above the thorax of the patient. A computer unit (KRUPP EPR 2500) with magnetic tapes and discs is available for data processing and data storage. Sequential images of the heart are taken over a period of 2 min in a two-second-frame mode and stored on magnetic tape. The total image was divided into 5 - 5 mm areas. From each area a functional curve was registered and the Myocardial Blood Flow (ml/min/100 g myocard) could be calculated from that. For a precise evaluation of the image of the Myocardial Blood Flow, any influences originating from other regions of the body had to be excluded. Therefore all curves are rejected which did not (a) reach their maximum value within 15s after injection, or (b) where the maximum was less than 10% of the highest maximum value of the reference curve. The values of the Myocardial Blood Flow calculated in this manner are displayed on a TV-screen. These values can be transformed into 10 colors or grey levels. This method has been proven to be useful in clinical practice for functional studies, especially for the judgement of the effectivity of pharmaceuticals. The quantitative display technique of the \"Myocardial Blood Flow\" improves the clinical statement in comparison to a scintigraphic picture.", "contents": "Quantitative scintigraphic display of myocardial blood flow. Technique and clinical evaluation. Immediately after coronarography 10 mCi 133Xenon were injected into the left or right coronary artery. A scintillation camera was placed above the thorax of the patient. A computer unit (KRUPP EPR 2500) with magnetic tapes and discs is available for data processing and data storage. Sequential images of the heart are taken over a period of 2 min in a two-second-frame mode and stored on magnetic tape. The total image was divided into 5 - 5 mm areas. From each area a functional curve was registered and the Myocardial Blood Flow (ml/min/100 g myocard) could be calculated from that. For a precise evaluation of the image of the Myocardial Blood Flow, any influences originating from other regions of the body had to be excluded. Therefore all curves are rejected which did not (a) reach their maximum value within 15s after injection, or (b) where the maximum was less than 10% of the highest maximum value of the reference curve. The values of the Myocardial Blood Flow calculated in this manner are displayed on a TV-screen. These values can be transformed into 10 colors or grey levels. This method has been proven to be useful in clinical practice for functional studies, especially for the judgement of the effectivity of pharmaceuticals. The quantitative display technique of the \"Myocardial Blood Flow\" improves the clinical statement in comparison to a scintigraphic picture."}
{"id": "PMID:1087906", "title": "Structure-activity relationship of some steroidal compounds in ocular inflammation.", "content": "The structure-activity relationship of some topically applied 21-desoxy-substituted steroids as anti-inflammatory agents was investigated; the anti-inflammatory activity remained unaltered or increased on introducing a methyl group in position 6alpha and a hydroxyl group in position 11beta, even after eliminating the hydroxyl group in position 21. The activity of the molecule was further increased by the introduction of a hydroxyl group in position 17alpha and a fluorine atom in position 9alpha.", "contents": "Structure-activity relationship of some steroidal compounds in ocular inflammation. The structure-activity relationship of some topically applied 21-desoxy-substituted steroids as anti-inflammatory agents was investigated; the anti-inflammatory activity remained unaltered or increased on introducing a methyl group in position 6alpha and a hydroxyl group in position 11beta, even after eliminating the hydroxyl group in position 21. The activity of the molecule was further increased by the introduction of a hydroxyl group in position 17alpha and a fluorine atom in position 9alpha."}
{"id": "PMID:1087908", "title": "[Influence of atropine on the chronotropic effects of stimulating the parasympathetic innervation of the heart].", "content": "Changes of the positive chronotropic effect in the frog atrium during stimulation of the extracardiac pathways or of the central end of intracardiac innervation after application of atropin (10(-4)--10(-5) on the sinus node, were studied. The positive chronotropic effects were abolished by the atropin application. The postganglionic impulse activity of the intracardiac nervous pathways did not change. Atropin had no influence on the sympathetic acceleration of the heart rate due to stimulation of sympathetic pathways. Positive chronotropic effects induced by stimulation of the parasympathetic extra or intracardiac pathways, are of cholinergic nature.", "contents": "[Influence of atropine on the chronotropic effects of stimulating the parasympathetic innervation of the heart]. Changes of the positive chronotropic effect in the frog atrium during stimulation of the extracardiac pathways or of the central end of intracardiac innervation after application of atropin (10(-4)--10(-5) on the sinus node, were studied. The positive chronotropic effects were abolished by the atropin application. The postganglionic impulse activity of the intracardiac nervous pathways did not change. Atropin had no influence on the sympathetic acceleration of the heart rate due to stimulation of sympathetic pathways. Positive chronotropic effects induced by stimulation of the parasympathetic extra or intracardiac pathways, are of cholinergic nature."}
{"id": "PMID:1087909", "title": "[Effect of ATP on the sensitivity of the from heart to adrenaline].", "content": "Adenosinthreephosphoric acid (ATP) in concentration 1-10(-6)--1-10(-5) g/ml decreases sensitivity of the frog heart muscle to adrenaline by half. It is suggested that ATP present in the specific granules of adrenals, c. n. s. and adrenergic axons together with catecholamines and excreted with the mediator during excitation of adrenergic nerve, alters the sensitivity of postsynaptic membrane to adrenaline.", "contents": "[Effect of ATP on the sensitivity of the from heart to adrenaline]. Adenosinthreephosphoric acid (ATP) in concentration 1-10(-6)--1-10(-5) g/ml decreases sensitivity of the frog heart muscle to adrenaline by half. It is suggested that ATP present in the specific granules of adrenals, c. n. s. and adrenergic axons together with catecholamines and excreted with the mediator during excitation of adrenergic nerve, alters the sensitivity of postsynaptic membrane to adrenaline."}
{"id": "PMID:1087910", "title": "Ultrastructure of macrophage-lymphocyte interaction in purpura pigmentosa progressiva.", "content": "Thin sections of pathologically changed skin lesions of patients suffering from purpura pigmentosa progressiva, a disorder which seems to be mediated by delayed type of allergy, were electron microscopically investigated. In 5 of the 7 cases we found cell infiltrates of the dermis consisting of many lymphocytes frequently in apposition to macrophages (histiocytes). The cell interactions observed occurred either by microvilli-like cytoplasmic protuberances or by local areas of the plasma membrane apposition. No submicroscopical alterations of the cytoplasm were found in the interacting cells although numerous macrophages show abundant microfilaments. The findings observed are interpreted as the ultrastructural substrate of cell cooperation for presenting and transfering antigens from macrophages to T lymphocytes. However, the question concerning the mechanism of the transfer remains open.", "contents": "Ultrastructure of macrophage-lymphocyte interaction in purpura pigmentosa progressiva. Thin sections of pathologically changed skin lesions of patients suffering from purpura pigmentosa progressiva, a disorder which seems to be mediated by delayed type of allergy, were electron microscopically investigated. In 5 of the 7 cases we found cell infiltrates of the dermis consisting of many lymphocytes frequently in apposition to macrophages (histiocytes). The cell interactions observed occurred either by microvilli-like cytoplasmic protuberances or by local areas of the plasma membrane apposition. No submicroscopical alterations of the cytoplasm were found in the interacting cells although numerous macrophages show abundant microfilaments. The findings observed are interpreted as the ultrastructural substrate of cell cooperation for presenting and transfering antigens from macrophages to T lymphocytes. However, the question concerning the mechanism of the transfer remains open."}
{"id": "PMID:1087912", "title": "Inhibition of human platelet aggregation by the proteolytic effect of streptokinase. Role of the human factor-VIII-related protein.", "content": "Defective ADP-induced aggregation was observed in in vitro streptokinases(SK)-treated normal platelet-rich plasma. Classic haemophilia and normal platelet poor plasma (PPP) treated with SK inhibit the aggregation of washed platelets; plasmin-treated normal human serum also shows an inhibitory effect on platelet aggregation. However, SK-treated von Willebrand plasmas do not inhibit the aggregation of washed platelets. This confirms the fact that the anti-aggregating effect is mainly linked to the digested factor VIII) but not to the digested fibrinogen. Defective ristocetin-induced platelet aggregation has also been observed in SK-treated plasmas. The presence of normal PPP does not modify the inhibition of the ADP-induced aggregation of washed platelets in SK-treated PPP. However, it does correct the ristocetin-induced aggregation. These results suggest that the inhibition of the ADP-induced aggregation is caused by the factor VIII degradation products, while the inhibition of the ristocetin-induced aggregation appears because of a defective von Willebrand activity of the factor VIII molecule.", "contents": "Inhibition of human platelet aggregation by the proteolytic effect of streptokinase. Role of the human factor-VIII-related protein. Defective ADP-induced aggregation was observed in in vitro streptokinases(SK)-treated normal platelet-rich plasma. Classic haemophilia and normal platelet poor plasma (PPP) treated with SK inhibit the aggregation of washed platelets; plasmin-treated normal human serum also shows an inhibitory effect on platelet aggregation. However, SK-treated von Willebrand plasmas do not inhibit the aggregation of washed platelets. This confirms the fact that the anti-aggregating effect is mainly linked to the digested factor VIII) but not to the digested fibrinogen. Defective ristocetin-induced platelet aggregation has also been observed in SK-treated plasmas. The presence of normal PPP does not modify the inhibition of the ADP-induced aggregation of washed platelets in SK-treated PPP. However, it does correct the ristocetin-induced aggregation. These results suggest that the inhibition of the ADP-induced aggregation is caused by the factor VIII degradation products, while the inhibition of the ristocetin-induced aggregation appears because of a defective von Willebrand activity of the factor VIII molecule."}
{"id": "PMID:1087913", "title": "Measurement of 5-hydroxyindole compounds during L-5-HTP treatment in depressed patients.", "content": "L-5-hydroxytryptophan (L-5-HTP), an immediate serotonin precursor, was given to the hospitalized depressed patients in an open clinical trial of the Phase 2 study for antidepressive effects of the agent. A relatively small dose, 150mg orally for seven days, was employed, and seven of 14 patients responded to the treatment with mild or moderate emelioration of their depressive symptoms. Urinary excretion levels and plasma concentrations of three 5-hydroxyindole compounds, 5-HTP, 5-HT and 5-HIAA, were measured during the drug treatment. Approximately 70% of the orally administered dose of L-5-HTP was recovered from the urine of depressed patients. Major part of urinary indoleamine metabolites was free and conjugate 5-HIAA. Excretion levels of these compounds in urine were not consistenly altered in the depressed patients as compared to those in normal subjects. Clinical response to L-5-HTP treatment appeared to have some correlation with the biochemical measures in the depressed patients, that is, non-responders exhibited significantly lower excretion levels of 5-HT and 5-HIAA in urine, and lower plasma levels of 5-HT than responders. Administered L-5-HTP may not be fully utilized in the depressed patients who did not react to the agent.", "contents": "Measurement of 5-hydroxyindole compounds during L-5-HTP treatment in depressed patients. L-5-hydroxytryptophan (L-5-HTP), an immediate serotonin precursor, was given to the hospitalized depressed patients in an open clinical trial of the Phase 2 study for antidepressive effects of the agent. A relatively small dose, 150mg orally for seven days, was employed, and seven of 14 patients responded to the treatment with mild or moderate emelioration of their depressive symptoms. Urinary excretion levels and plasma concentrations of three 5-hydroxyindole compounds, 5-HTP, 5-HT and 5-HIAA, were measured during the drug treatment. Approximately 70% of the orally administered dose of L-5-HTP was recovered from the urine of depressed patients. Major part of urinary indoleamine metabolites was free and conjugate 5-HIAA. Excretion levels of these compounds in urine were not consistenly altered in the depressed patients as compared to those in normal subjects. Clinical response to L-5-HTP treatment appeared to have some correlation with the biochemical measures in the depressed patients, that is, non-responders exhibited significantly lower excretion levels of 5-HT and 5-HIAA in urine, and lower plasma levels of 5-HT than responders. Administered L-5-HTP may not be fully utilized in the depressed patients who did not react to the agent."}
{"id": "PMID:1087914", "title": "Reduction of blood platelet serotonin levels in manic and depressed patients.", "content": "Blood platelet serotonin levels were measured in unmedicated 12 manic and 74 depressive patients with 118 normal control subjects employed. Blood platelets were separated by multiple contrifugation in the medium of Na2-EDTA solution, and the loss of serotonin during collecting procedures was about 11%. The mean value of blood platelet serotonin levels in depressed patients was 594+/-288ng/mg platelet protein (+/- S.D.), which was significantly lower than that for normal controls, 780 +/- 253ng/mg protein (p less than 0.001). Age does not account for the reduction of serotonin levels both in depressed and in normal population. Unipolar and involutional depressed patients exhibited to have the most pronounced reduced levels of serotonin of various subtypes of depression, while bipolar depressed patients, neurotic and chronic characterological depressed patients as well as patients with first-episode depression had the values which were comparable with those in normal controls. Manic patients did not show enhancement but did reduction of serotonin levels, the mean being 580+/-152ng/mg protein, which made a contrast with their clinical manifestations of exhilaration and hyperactivity. Changes in blood platelet serotonin levels were determined before, during and after administration of L-5-HTP with a maintenance dose of 300mg daily in nine depressed patients. Serotonin levels in all subjects were lifted to normal levels during the L-5-HTP treatment, while clinical symptoms were not improved with the treatment. Reduction of blood platelet serotonin levels in depressed patients may be due to their psychobiological distinction, which involves abnormal biogenic amine metabolism in the brain.", "contents": "Reduction of blood platelet serotonin levels in manic and depressed patients. Blood platelet serotonin levels were measured in unmedicated 12 manic and 74 depressive patients with 118 normal control subjects employed. Blood platelets were separated by multiple contrifugation in the medium of Na2-EDTA solution, and the loss of serotonin during collecting procedures was about 11%. The mean value of blood platelet serotonin levels in depressed patients was 594+/-288ng/mg platelet protein (+/- S.D.), which was significantly lower than that for normal controls, 780 +/- 253ng/mg protein (p less than 0.001). Age does not account for the reduction of serotonin levels both in depressed and in normal population. Unipolar and involutional depressed patients exhibited to have the most pronounced reduced levels of serotonin of various subtypes of depression, while bipolar depressed patients, neurotic and chronic characterological depressed patients as well as patients with first-episode depression had the values which were comparable with those in normal controls. Manic patients did not show enhancement but did reduction of serotonin levels, the mean being 580+/-152ng/mg protein, which made a contrast with their clinical manifestations of exhilaration and hyperactivity. Changes in blood platelet serotonin levels were determined before, during and after administration of L-5-HTP with a maintenance dose of 300mg daily in nine depressed patients. Serotonin levels in all subjects were lifted to normal levels during the L-5-HTP treatment, while clinical symptoms were not improved with the treatment. Reduction of blood platelet serotonin levels in depressed patients may be due to their psychobiological distinction, which involves abnormal biogenic amine metabolism in the brain."}
{"id": "PMID:1087916", "title": "[Intracellular endobiosis as a basic factor in acquired immunity in man (author's transl)].", "content": "An attempt is made to explain the significance of intracellular endobiosis for the human acquisition of immunity against cyclic infections diseases. It is pointed out that, taking into account the temporally limited reduplication of an immune-potent cell clone, a species-specific induction of immunity against infections and other diseases is sustained for a sufficiently long period of time only by a microorganism which is still living.", "contents": "[Intracellular endobiosis as a basic factor in acquired immunity in man (author's transl)]. An attempt is made to explain the significance of intracellular endobiosis for the human acquisition of immunity against cyclic infections diseases. It is pointed out that, taking into account the temporally limited reduplication of an immune-potent cell clone, a species-specific induction of immunity against infections and other diseases is sustained for a sufficiently long period of time only by a microorganism which is still living."}
{"id": "PMID:1087917", "title": "5-bromodeoxyuridine-light inactivation of human lymphocytes stimulated mitogens and allogeneic cells: evidence for distinct T-lymphocyte subsets.", "content": "The study of human peripheral blood currently permits enumeration of circulating B and T lymphocytes as well as the analysis of functional responses in vitro following stimulation with mitogens, antigens or allogeneic cells. In the present experiments, subsets of these major lymphocyte populations were analyzed by dissecting in vitro responses using an ablative technique. After an initial culture period of lymphocytes with a mitogen, the proliferating cells were inactivated with 5-bromodeoxyuridine and light, then the capacity of the remaining lymphocytes to respond to the same or a different mitogenic influence was tested. Responsiveness to a different stimulant in the presence of no further response to the first stimulant was taken as evidence for a different responding cell population. A large subset of peripheral blood lymphocytes was responsive to both phytohemagglutinin (PHA) and concanavalin A (Con A); ablation of the cells responsive to one left little or no cells responsive to the other. Pokeweed mitogen (PWM) stimulated a portion of the PHA-Con A-responsive subset and an approximately equal subset unresponsive to PHA or Con A. Other evidence indicates that with each of these mitogens (especially with PHA and Con A in a soluble form), most of the proliferative response of peripheral blood B lymphocytes is indirectly triggered and is dependent on T cell stimulation. The population of PHA-Con A-responsive cells is, therefore, interpreted to represent a major T cell subset plus recruited cells; the PWM-responsive population would include a T cell subset having also PHA and Con A responsiveness, and another subset of T (or perhaps B) cells. The mitogen-sensitive population showed no overlap with cells responsive to allogeneic stimulation in mixed leukocyte culture. Ablation of the mitogen-responsive cells potentiated the mixed leukocyte reaction, suggesting that a suppressive influence was removed with the inactivation of the mitogen-responsive cells. It appears, therefore, that distinct subsets of T lymphocytes differentially responsive to PHA-Con A, to PWM and to allogeneic stimulation are present in the human peripheral blood.", "contents": "5-bromodeoxyuridine-light inactivation of human lymphocytes stimulated mitogens and allogeneic cells: evidence for distinct T-lymphocyte subsets. The study of human peripheral blood currently permits enumeration of circulating B and T lymphocytes as well as the analysis of functional responses in vitro following stimulation with mitogens, antigens or allogeneic cells. In the present experiments, subsets of these major lymphocyte populations were analyzed by dissecting in vitro responses using an ablative technique. After an initial culture period of lymphocytes with a mitogen, the proliferating cells were inactivated with 5-bromodeoxyuridine and light, then the capacity of the remaining lymphocytes to respond to the same or a different mitogenic influence was tested. Responsiveness to a different stimulant in the presence of no further response to the first stimulant was taken as evidence for a different responding cell population. A large subset of peripheral blood lymphocytes was responsive to both phytohemagglutinin (PHA) and concanavalin A (Con A); ablation of the cells responsive to one left little or no cells responsive to the other. Pokeweed mitogen (PWM) stimulated a portion of the PHA-Con A-responsive subset and an approximately equal subset unresponsive to PHA or Con A. Other evidence indicates that with each of these mitogens (especially with PHA and Con A in a soluble form), most of the proliferative response of peripheral blood B lymphocytes is indirectly triggered and is dependent on T cell stimulation. The population of PHA-Con A-responsive cells is, therefore, interpreted to represent a major T cell subset plus recruited cells; the PWM-responsive population would include a T cell subset having also PHA and Con A responsiveness, and another subset of T (or perhaps B) cells. The mitogen-sensitive population showed no overlap with cells responsive to allogeneic stimulation in mixed leukocyte culture. Ablation of the mitogen-responsive cells potentiated the mixed leukocyte reaction, suggesting that a suppressive influence was removed with the inactivation of the mitogen-responsive cells. It appears, therefore, that distinct subsets of T lymphocytes differentially responsive to PHA-Con A, to PWM and to allogeneic stimulation are present in the human peripheral blood."}
{"id": "PMID:1087918", "title": "Cellular requirements for simulation by the purified streptococcal antigen DSR.", "content": "Delayed skin reactor (DSR) was prepared from streptokinase-streptodornase. Peripheral blood mononuclear cells, were collected from a Ficoll-Hypaque gradient and subsequently subdivided into T cell-, B cell-, or monocyte-depleted populations. Mononuclear cells with complement receptors (B cells) were removed by a nylon-wool column adsorption. Sheep erythrocyte rosette forming cells (T cells) were removed by centrifugation on a second gradient. Populations of cells which did not adhere to plastic and/or ingest iron filings were monocyte-depleted. While response to DSR as measured by incorporation of tritiated thymidine into DNA was not affected by removing B lymphocytes, it was significantly diminished by removing T lymphocytes. Monocyte-depleted cells incorporated less tritiated thymidine into DNA in both antigen stimulated and control cells, but the stimulation index was not significantly altered.", "contents": "Cellular requirements for simulation by the purified streptococcal antigen DSR. Delayed skin reactor (DSR) was prepared from streptokinase-streptodornase. Peripheral blood mononuclear cells, were collected from a Ficoll-Hypaque gradient and subsequently subdivided into T cell-, B cell-, or monocyte-depleted populations. Mononuclear cells with complement receptors (B cells) were removed by a nylon-wool column adsorption. Sheep erythrocyte rosette forming cells (T cells) were removed by centrifugation on a second gradient. Populations of cells which did not adhere to plastic and/or ingest iron filings were monocyte-depleted. While response to DSR as measured by incorporation of tritiated thymidine into DNA was not affected by removing B lymphocytes, it was significantly diminished by removing T lymphocytes. Monocyte-depleted cells incorporated less tritiated thymidine into DNA in both antigen stimulated and control cells, but the stimulation index was not significantly altered."}
{"id": "PMID:1087919", "title": "Occurrence of suppressor cells in lymph nodes and spleen at later times after immunization with contact-sensitizing agent picryl chloride.", "content": "The lymph node and spleen cells of mice painted on the skin with the contact sensitizing agent, picryl chloride, transfer contact sensitivity. Their ability to transfer reaches a peak 4 days after immunization and is absent by day 6 providing the recipient mice are challenged shortly after transfer (Chase type transfer). In contrast, when challenge of the recipients is delayed for 6 days (adoptive transfer), lymph node and spleen cells show the greatest ability to transfer 8-12 days after immunization. When cells taken 4 days after immunization (which transfer contact sensitivity) are mixed with cells taken at 6-11 days (which fail to transfer), the mixture shows little ability to transfer. This provides evidence for the occurrence of suppressor cells. Lymph node and spleen, and thymus cells show suppressor activity. The suppression is specific and cells from donors immunized with the contact-sensitizing agent oxazolone will not suppress passive transfer of contuse of the loss of ability of lymph node and spleen cells in transfer later than day 6 after immunization. Experiments on the loss of radioactivity from lymph nodes labelled with 125I-iododeoxyuridine (IUDR) suggest that loss of cells from the lymph nodes may be a contributory factor.", "contents": "Occurrence of suppressor cells in lymph nodes and spleen at later times after immunization with contact-sensitizing agent picryl chloride. The lymph node and spleen cells of mice painted on the skin with the contact sensitizing agent, picryl chloride, transfer contact sensitivity. Their ability to transfer reaches a peak 4 days after immunization and is absent by day 6 providing the recipient mice are challenged shortly after transfer (Chase type transfer). In contrast, when challenge of the recipients is delayed for 6 days (adoptive transfer), lymph node and spleen cells show the greatest ability to transfer 8-12 days after immunization. When cells taken 4 days after immunization (which transfer contact sensitivity) are mixed with cells taken at 6-11 days (which fail to transfer), the mixture shows little ability to transfer. This provides evidence for the occurrence of suppressor cells. Lymph node and spleen, and thymus cells show suppressor activity. The suppression is specific and cells from donors immunized with the contact-sensitizing agent oxazolone will not suppress passive transfer of contuse of the loss of ability of lymph node and spleen cells in transfer later than day 6 after immunization. Experiments on the loss of radioactivity from lymph nodes labelled with 125I-iododeoxyuridine (IUDR) suggest that loss of cells from the lymph nodes may be a contributory factor."}
{"id": "PMID:1087920", "title": "T and B lymphocyte populations in human normal lymph node, regional tumour lymph node and inflammatory lymph node.", "content": "We have determined the T and B lymphocyte subpopulations in normal human lymph nodes. The lymphocyte profile was the same irrespective of the anatomical distribution and was similar to that found in peripheral blood with identical T and B cell values but with a lower Fc and a higher C3-receptor-bearing lymphocyte subpopulation. This pattern showed a marked change in the regional nodes of patients with mammary carcinoma and nodes draining a variety of other solid tumours, with a fall in T and pronounced elevation of B, Fc and C3 cells but with a persistence of C3 predominance. The lymphocyte profile found in tonsils and nodes draining inflammatory foci was a similar but further exaggeration of the tumour node pattern, with reversal of T and B cell ratios. The T and B lymphocyte percentages in the peripheral blood of patients with clinically localized breast cancer are identical to those of the healthy controls. Different Fc/C3 subsets exist in peripheral blood and lymphoid structures and probably represent a differential functional heterogeneity. Proximity of tumour to the draining node modified this profile.", "contents": "T and B lymphocyte populations in human normal lymph node, regional tumour lymph node and inflammatory lymph node. We have determined the T and B lymphocyte subpopulations in normal human lymph nodes. The lymphocyte profile was the same irrespective of the anatomical distribution and was similar to that found in peripheral blood with identical T and B cell values but with a lower Fc and a higher C3-receptor-bearing lymphocyte subpopulation. This pattern showed a marked change in the regional nodes of patients with mammary carcinoma and nodes draining a variety of other solid tumours, with a fall in T and pronounced elevation of B, Fc and C3 cells but with a persistence of C3 predominance. The lymphocyte profile found in tonsils and nodes draining inflammatory foci was a similar but further exaggeration of the tumour node pattern, with reversal of T and B cell ratios. The T and B lymphocyte percentages in the peripheral blood of patients with clinically localized breast cancer are identical to those of the healthy controls. Different Fc/C3 subsets exist in peripheral blood and lymphoid structures and probably represent a differential functional heterogeneity. Proximity of tumour to the draining node modified this profile."}
{"id": "PMID:1087921", "title": "Characterization of K-cell activity by use of depletion experiments.", "content": "Normal human blood lymphocytes with affinity for ox red cells sensitized with IgG antibody, for normal or papain-treated sheep red cells and for ox red cells coated with mouse complement were used for rosette formation and the rosetting cells separated by density gradient centrifugation on Ficoll/hypaque. The non-rosetting cells at the interface were collected and compared with cell suspensions before treatment for direct and antibody-dependent cytotoxicity of human target cells. Depletion of Fc-receptor-bearing lymphocytes strongly decreased antibody-dependent cell-mediated cytotoxicity; reduction in the number (or depletion) of T cells and cells with C'3 receptor had no effect, showing the same or enhanced K-cell activity. It is concluded that one type of K or killer cell has Fc receptors but lacks C'3 receptors.", "contents": "Characterization of K-cell activity by use of depletion experiments. Normal human blood lymphocytes with affinity for ox red cells sensitized with IgG antibody, for normal or papain-treated sheep red cells and for ox red cells coated with mouse complement were used for rosette formation and the rosetting cells separated by density gradient centrifugation on Ficoll/hypaque. The non-rosetting cells at the interface were collected and compared with cell suspensions before treatment for direct and antibody-dependent cytotoxicity of human target cells. Depletion of Fc-receptor-bearing lymphocytes strongly decreased antibody-dependent cell-mediated cytotoxicity; reduction in the number (or depletion) of T cells and cells with C'3 receptor had no effect, showing the same or enhanced K-cell activity. It is concluded that one type of K or killer cell has Fc receptors but lacks C'3 receptors."}
{"id": "PMID:1087922", "title": "E and EAC rosette-forming cells in healthy donors and cancer patients.", "content": "The influence of age and sex on the lymphocyte count and numbers of E and EAC rosette-forming cells (RFC) was investigated using peripheral blood of healthy donors. In the female donors we found a lower total lymphocyte count and percentage of EAC RFC than in male donors. The percentage and total number of E RFC appeared to be low in younger donors. Total lymphocyte count and numbers of E and EAC RFC were investigated in untreated and treated patients with advanced solid tumors and in patients with untreated malignant lymphomas. The lymphocyte count in all these groups was decreased, but the E/EAC RFC ratio was normal, except in the group with untreated solid tumors, where we found an increased percentage of E RFC. It is suggested that in these patients a nonspecific impairment of bone marrow function might be expressed in numbers of peripheral T and B cells.", "contents": "E and EAC rosette-forming cells in healthy donors and cancer patients. The influence of age and sex on the lymphocyte count and numbers of E and EAC rosette-forming cells (RFC) was investigated using peripheral blood of healthy donors. In the female donors we found a lower total lymphocyte count and percentage of EAC RFC than in male donors. The percentage and total number of E RFC appeared to be low in younger donors. Total lymphocyte count and numbers of E and EAC RFC were investigated in untreated and treated patients with advanced solid tumors and in patients with untreated malignant lymphomas. The lymphocyte count in all these groups was decreased, but the E/EAC RFC ratio was normal, except in the group with untreated solid tumors, where we found an increased percentage of E RFC. It is suggested that in these patients a nonspecific impairment of bone marrow function might be expressed in numbers of peripheral T and B cells."}
{"id": "PMID:1087923", "title": "Studies on immune responses to parasite antigens in mice. I. Ascaris suum larvae numbers and antiphosphorylcholine responses in infected mice of various strains and in hypothymic nu/nu mice.", "content": "In terms of day 7 lung larvae numbers, mice vary markedly in their suscepibility to a first infection with the nematode worms, Ascaris suum, and the highly susceptible strain, C57Bl, is resistant to second infection. Time course studies suggested that the period of residence in the liver or migration to, or into, the lungs are stages of the life cycle in which natural or acquired resistance of the host is expressed. The traits, susceptibility and resistance to first infection, were under polygenic control and no linkage of susceptibility to the major histocompatibility complex of C57Bl mice (H-2b) was observed. Acquired resistance (to second infection) has not been dissected because of our inability to show adoptive transfer of resistance to naive recipeints. Studies in hypothymic BALB/c. nu/nu mice indicate that natural resistance (to first infection) is not affected by a lack of T cells. The T cell dependence of acquired resistance in C57Bl mice remains in doubt although in the relatively resistant strain BALB/c, hypothymic nu/nu mice after second infection contain as many larvae in their lungs and liver as are present after first infection. An eosinophilia is observed in infected intact mice but not in infected T cell-deficient mice. Partially T cell-dependent serum antibodies and plaque-forming cells to phosphorylcholine (PC) were present in mice infected with A. suum but no evidence was obtained that this anti-PC antibody response was in any way protective for the host. The cell membrane-acitive properties of PC and related molecules suggest that PC-containing parasite antigens may be tolerogens for certain of the B cells with specificity for parasite antigens. A state of partial tolerance involving high affinity antibody production may be one means whereby parasites survive in natural or unnatural hosts.", "contents": "Studies on immune responses to parasite antigens in mice. I. Ascaris suum larvae numbers and antiphosphorylcholine responses in infected mice of various strains and in hypothymic nu/nu mice. In terms of day 7 lung larvae numbers, mice vary markedly in their suscepibility to a first infection with the nematode worms, Ascaris suum, and the highly susceptible strain, C57Bl, is resistant to second infection. Time course studies suggested that the period of residence in the liver or migration to, or into, the lungs are stages of the life cycle in which natural or acquired resistance of the host is expressed. The traits, susceptibility and resistance to first infection, were under polygenic control and no linkage of susceptibility to the major histocompatibility complex of C57Bl mice (H-2b) was observed. Acquired resistance (to second infection) has not been dissected because of our inability to show adoptive transfer of resistance to naive recipeints. Studies in hypothymic BALB/c. nu/nu mice indicate that natural resistance (to first infection) is not affected by a lack of T cells. The T cell dependence of acquired resistance in C57Bl mice remains in doubt although in the relatively resistant strain BALB/c, hypothymic nu/nu mice after second infection contain as many larvae in their lungs and liver as are present after first infection. An eosinophilia is observed in infected intact mice but not in infected T cell-deficient mice. Partially T cell-dependent serum antibodies and plaque-forming cells to phosphorylcholine (PC) were present in mice infected with A. suum but no evidence was obtained that this anti-PC antibody response was in any way protective for the host. The cell membrane-acitive properties of PC and related molecules suggest that PC-containing parasite antigens may be tolerogens for certain of the B cells with specificity for parasite antigens. A state of partial tolerance involving high affinity antibody production may be one means whereby parasites survive in natural or unnatural hosts."}
{"id": "PMID:1087924", "title": "Studies on immune responses to parasite antigens in mice. II. Aspects of the T cell dependence of circulating reagin production to Ascaris suum antigens.", "content": "Heat-labile, rat skin-fixing antibodies were detected readily in the sera of young female mice dosed intranasally with the body fluid of Ascaris suum (ABF) and the adjuvant, Bordetella pertussis vaccine (BPV). In addition, washed cell suspensions prepared from spleen and the lymph nodes regional to the lungs were positive in an adoptive cutaneous anaphylaxis assay, an assay which may detect activities of reagins associated with mast cells rather than reaginic antibody-secreting cells. The intraperitoneal route was a poor means of inducing circulating anti-ABF reagins and an intraperitoneal injection of ABF + BPV delayed the appearance of circulating reagins in mice dosed at the same time with ABF + BPV intranasally. Hypothymic female BALB/c. nu/nu ('nude') mice failed to produce circulating reagins to ABF but an injection of normal thymocytes or cortisone-resistant thymocytes from syngeneic female mice led to higher titers of circulating reagins than found in normal female BALB/c. nu/+ littermates. Using cells from young male or female syngeneic donors and male and female BALB/c. nu/mu recpiients, evidence was obtained for a defect in the thymus of young male mice and conceivably this defect may extend to the peripheral T cell population in such mice. Cyclophosphamide pretreatment or adrenalectomy increased circulating reagin titers in normal mice dosed intranasally with ABF + BPV, and pretreatment with lipopolysaccharide intranasally markedly reduced titers of circulating anti-ABF reagins. In the discussion, emphasis is given to the hypothesis that potent allergens are T cell-stimulating, relatively persistent antigens which, when located in submucosal lymphoid sites and under conditions of limited antibody production as a result of limited recruitment of 'helper' T cells systemically, lead to the induction and sustained production of IgE by resident Bxi cells and their progeny.", "contents": "Studies on immune responses to parasite antigens in mice. II. Aspects of the T cell dependence of circulating reagin production to Ascaris suum antigens. Heat-labile, rat skin-fixing antibodies were detected readily in the sera of young female mice dosed intranasally with the body fluid of Ascaris suum (ABF) and the adjuvant, Bordetella pertussis vaccine (BPV). In addition, washed cell suspensions prepared from spleen and the lymph nodes regional to the lungs were positive in an adoptive cutaneous anaphylaxis assay, an assay which may detect activities of reagins associated with mast cells rather than reaginic antibody-secreting cells. The intraperitoneal route was a poor means of inducing circulating anti-ABF reagins and an intraperitoneal injection of ABF + BPV delayed the appearance of circulating reagins in mice dosed at the same time with ABF + BPV intranasally. Hypothymic female BALB/c. nu/nu ('nude') mice failed to produce circulating reagins to ABF but an injection of normal thymocytes or cortisone-resistant thymocytes from syngeneic female mice led to higher titers of circulating reagins than found in normal female BALB/c. nu/+ littermates. Using cells from young male or female syngeneic donors and male and female BALB/c. nu/mu recpiients, evidence was obtained for a defect in the thymus of young male mice and conceivably this defect may extend to the peripheral T cell population in such mice. Cyclophosphamide pretreatment or adrenalectomy increased circulating reagin titers in normal mice dosed intranasally with ABF + BPV, and pretreatment with lipopolysaccharide intranasally markedly reduced titers of circulating anti-ABF reagins. In the discussion, emphasis is given to the hypothesis that potent allergens are T cell-stimulating, relatively persistent antigens which, when located in submucosal lymphoid sites and under conditions of limited antibody production as a result of limited recruitment of 'helper' T cells systemically, lead to the induction and sustained production of IgE by resident Bxi cells and their progeny."}
{"id": "PMID:1087925", "title": "Studies on immune responses to parasite antigens in mice. III. Nippostrongylus brasiliensis infections in hypothymic nu/nu mice.", "content": "Normal mice of several strains reject the nematode worm Nippostronglyus brasiliensis from the intestine within 14 days (and most often within 10 days) of injection of high numbers of infective third stage larvae (L3). Previously-infected mice are markedly resistant to a second infection. Hypothymic, nu/nu ('nude') mice continue to harbor worms long after intact mice have rejected the worm burden and an inoculum of T cells at the time of, or several days after L3 injection, leads to worm elimination within 14 days in nu/nu mice. As yet no evidence is available to indicate whether or not T cells with specificity for parasite antigens are required in a reconstitutive inoculum in nu/nu mice. Pretreatment of normal mice with cyclophosphamide, at doses reported to lead to temporary B cell hypofunction, does not result in delayed rejection of worms but the participation of B cell products (antibodies) cannot be discounted on the basis of this result. The nu/nu mouse - N. brasiliensis system will be useful in the search for, and characterization of, parasite antigens which gain access to the lymphoid organs and circulation of parasitized mice and in the analysis of T cell subtypes (both functional and surface Thy and Ly alloantigenic subtypes) involved in worm elimination.", "contents": "Studies on immune responses to parasite antigens in mice. III. Nippostrongylus brasiliensis infections in hypothymic nu/nu mice. Normal mice of several strains reject the nematode worm Nippostronglyus brasiliensis from the intestine within 14 days (and most often within 10 days) of injection of high numbers of infective third stage larvae (L3). Previously-infected mice are markedly resistant to a second infection. Hypothymic, nu/nu ('nude') mice continue to harbor worms long after intact mice have rejected the worm burden and an inoculum of T cells at the time of, or several days after L3 injection, leads to worm elimination within 14 days in nu/nu mice. As yet no evidence is available to indicate whether or not T cells with specificity for parasite antigens are required in a reconstitutive inoculum in nu/nu mice. Pretreatment of normal mice with cyclophosphamide, at doses reported to lead to temporary B cell hypofunction, does not result in delayed rejection of worms but the participation of B cell products (antibodies) cannot be discounted on the basis of this result. The nu/nu mouse - N. brasiliensis system will be useful in the search for, and characterization of, parasite antigens which gain access to the lymphoid organs and circulation of parasitized mice and in the analysis of T cell subtypes (both functional and surface Thy and Ly alloantigenic subtypes) involved in worm elimination."}
{"id": "PMID:1087926", "title": "Unusual combination of immune and endocrine deficiencies. A possible case of early-onset Louis-Bar syndrome.", "content": "Immunodeficiency functionally limited to the B-cell system together with mild hypothyroidism and severe growth hormone deficiency was found in a 6 1/2-month-old female infant with recurrent infections and growth retardation. A lymph node biopsy and post mortem examination of the lymphoid organs surprisingly revealed severe deficiency of both thymus-dependent and bursa-equivalent systems. The unusual combination of immune and endocrine deficiencies posed a difficult diagnostic problem. The hypothesis of an early-onset Louis-Bar syndrome was suggested and apparently corroborated by the autopsy findings of ovarian dysgenesis and cerebellar degeneration. The dissociation between functional and morphological findings as regards the immunodeficiency, and the possible links between immune and endocrine derangements are discussed.", "contents": "Unusual combination of immune and endocrine deficiencies. A possible case of early-onset Louis-Bar syndrome. Immunodeficiency functionally limited to the B-cell system together with mild hypothyroidism and severe growth hormone deficiency was found in a 6 1/2-month-old female infant with recurrent infections and growth retardation. A lymph node biopsy and post mortem examination of the lymphoid organs surprisingly revealed severe deficiency of both thymus-dependent and bursa-equivalent systems. The unusual combination of immune and endocrine deficiencies posed a difficult diagnostic problem. The hypothesis of an early-onset Louis-Bar syndrome was suggested and apparently corroborated by the autopsy findings of ovarian dysgenesis and cerebellar degeneration. The dissociation between functional and morphological findings as regards the immunodeficiency, and the possible links between immune and endocrine derangements are discussed."}
{"id": "PMID:1087927", "title": "A comparative study of histochemical mapping on the distribution of acid phosphatase and 5-nucleotidase in the forebrain of frog (Rana tigrina).", "content": "The four well defined dorsal, lateral, medial and intermediate olfactory tracts of the frog have been studied. During their courses they become intermingled with fibers related to the primordial septum, the primordial hippocampus, the primordial dorsal pallium and the primordial piriform areas. In acid phosphatase preparations all these tracts are completely negative, and, therefore, are not identifiable. However, these tracts have intense staining reaction to 5-nucleotidase throughout their courses. Differences in the distribution of acid phosphatase and 5-nucleotidase are apparent in the hippocampal and anterior commissures. These are negative for acid phosphatase. In 5-nucleotidase preparations the anterior commissure has an intensely positive whereas the hippocampal commissure has a completely negative reaction. The medial septal nucleus is intensely positive for 5-nucleotidase at the anterior hemisphere level. There is a gradual decrease in intensity of staining in this nucleus towards the posterior hemisphere levels so that in the last few sections of the forebrain of the frog the medial septal nucleus is completely unstained or negative. In the acid phosphatase preparations at all levels the medial septal nucleus presents a moderately positive reaction. In 5-nucleotidase preparations, the dorsomedial part of primordial hippocampus is intensely positive whereas the dorsal part of the hippocampus is completely negative. In acid phosphatase preparations, both dorsal and dorsomedial parts of hippocampus are intensely positive. The striatal complex and the lateral septal nucleus are positive for acid phosphatase and negative for 5-nucleotidase. The possible significance of some of these similarities and differences in tracts and nuclei has been considered with reference to the functioning of the various parts. The present histoenzymological preparations have revealed distinct nerve pathways. In some instances clear-cut connections, which had hitherto been unrevealed by neuroanotomical methods, have been demonstrated.", "contents": "A comparative study of histochemical mapping on the distribution of acid phosphatase and 5-nucleotidase in the forebrain of frog (Rana tigrina). The four well defined dorsal, lateral, medial and intermediate olfactory tracts of the frog have been studied. During their courses they become intermingled with fibers related to the primordial septum, the primordial hippocampus, the primordial dorsal pallium and the primordial piriform areas. In acid phosphatase preparations all these tracts are completely negative, and, therefore, are not identifiable. However, these tracts have intense staining reaction to 5-nucleotidase throughout their courses. Differences in the distribution of acid phosphatase and 5-nucleotidase are apparent in the hippocampal and anterior commissures. These are negative for acid phosphatase. In 5-nucleotidase preparations the anterior commissure has an intensely positive whereas the hippocampal commissure has a completely negative reaction. The medial septal nucleus is intensely positive for 5-nucleotidase at the anterior hemisphere level. There is a gradual decrease in intensity of staining in this nucleus towards the posterior hemisphere levels so that in the last few sections of the forebrain of the frog the medial septal nucleus is completely unstained or negative. In the acid phosphatase preparations at all levels the medial septal nucleus presents a moderately positive reaction. In 5-nucleotidase preparations, the dorsomedial part of primordial hippocampus is intensely positive whereas the dorsal part of the hippocampus is completely negative. In acid phosphatase preparations, both dorsal and dorsomedial parts of hippocampus are intensely positive. The striatal complex and the lateral septal nucleus are positive for acid phosphatase and negative for 5-nucleotidase. The possible significance of some of these similarities and differences in tracts and nuclei has been considered with reference to the functioning of the various parts. The present histoenzymological preparations have revealed distinct nerve pathways. In some instances clear-cut connections, which had hitherto been unrevealed by neuroanotomical methods, have been demonstrated."}
{"id": "PMID:1087929", "title": "Histiocytosis X: a case report.", "content": "The rare disease entity of histocytosis X is presented. Features of unusual interests in the present case were the bilateral exophthalmus as the presenting sign, fever, ottorhoea and involvement of lung and long bones.", "contents": "Histiocytosis X: a case report. The rare disease entity of histocytosis X is presented. Features of unusual interests in the present case were the bilateral exophthalmus as the presenting sign, fever, ottorhoea and involvement of lung and long bones."}
{"id": "PMID:1087930", "title": "Corneal electroretinograms in children without sedation.", "content": "Many physicians assume that general anesthesia or sedation is necessary to perform electroretinograms (ERGs) on children and for this reason are reluctant to request the test. To challenge this assumption, routine contact lens ERGs, using topical anesthesia only, were attempted on 30 consecutively referred children (aged five months to six years). Mild restraint was sometimes needed, but successful oscilloscopic ERGs were obtained from all 30 children. The records were less stable than those from adults, but the response amplitude, wave-form and timing could always be evaluated and the cone and rod systems could be clearly differentiated.", "contents": "Corneal electroretinograms in children without sedation. Many physicians assume that general anesthesia or sedation is necessary to perform electroretinograms (ERGs) on children and for this reason are reluctant to request the test. To challenge this assumption, routine contact lens ERGs, using topical anesthesia only, were attempted on 30 consecutively referred children (aged five months to six years). Mild restraint was sometimes needed, but successful oscilloscopic ERGs were obtained from all 30 children. The records were less stable than those from adults, but the response amplitude, wave-form and timing could always be evaluated and the cone and rod systems could be clearly differentiated."}
{"id": "PMID:1087932", "title": "An evaluation of the membrane constants and the potassium conductance in metabolically exhausted muscle fibres.", "content": "1. The membrane characteristics of metabolically poisoned and mechanically exhausted frog skeletal muscle fibres were investigated with intracellular micro-electrodes. 2. When cyanide plus iodoacetate were applied as metabolic poisons twitch tension declined towards zero after 150-300 stimuli (0-3 Hz; temperature = 0 degrees C). At the beginning of stimulation the mean resting potenial fell from -75 to -69 mV; it rose subsequently to -83 mV. The membrane resistance decreased during this stimulation period along a sigmoid time course to 4-6% of the original value. 3. In completely exhausted fibres the following membrane constants were estimated (23 degrees C): length constant, 0-31 mm; input resistance, 31 komega; membrane resistance, 58 omega.cm2. These values were calculated under the assumption of a constant internal resistivity of 170 omega. cm. The Q10 values of these constants were similar to those in normal fibres. Afew experiments revealed that the membrane capacity remained roughly constant under these conditions. 4. The current-voltage relation of exhausted fibres was approximately linear in the range between -60 and -100 mV. At less negative potentials the conductance increased slightly while at more negative potentials it decreased. The latter, in particular, became more evident when the imput current was converted into membrane current density by applying Cole's theorem. 5TEA+ and Rb+ in the external solution increased the membrane resistance of exhausted fibres by more than one order of magnitude. The major part of the membrane conductance induced by exhaustion, however, could not be blocked by these ions or Zn2+. 6. Chloride-free test solutions were used to measure the relative contributions of potassium and chloride ions to the membrane conductance. The relation GK:GC1 changed from 2:3 in normal fibres to 5:1 in exhausted ones. In absolute terms GK rose from ca. 130 to 14,300 mumho/cm2 and GC1 from ca. 200 to 2900 mumho/cm2. The discrimination between K+ and Na+ by the resting membrane in exhausted fibres was probably equal to or even higher than that under normal conditions. 7. In normal fibres the input resistance decreased by up to 20% after the external application of 1-2 mM caffeine, which is known to release calcium ions from internal stores. The elevation in internal Ca2+ by direct injection caused a small and, as a rule, irreversible decrease in input resistance which was probably partly due to local damage to the surface membrane. 8. It is concluded that in metabolically exhausted muscle fibres the surface and tubular membranes are still intact and that the observed decrease in membrane resistance is mainly due to an increase in potassium conductance. In addition, the results indicate that the gating mechanism of the potassium channels (presumably those with the characteristics of the slow component) is affected when energy reserves diminish.", "contents": "An evaluation of the membrane constants and the potassium conductance in metabolically exhausted muscle fibres. 1. The membrane characteristics of metabolically poisoned and mechanically exhausted frog skeletal muscle fibres were investigated with intracellular micro-electrodes. 2. When cyanide plus iodoacetate were applied as metabolic poisons twitch tension declined towards zero after 150-300 stimuli (0-3 Hz; temperature = 0 degrees C). At the beginning of stimulation the mean resting potenial fell from -75 to -69 mV; it rose subsequently to -83 mV. The membrane resistance decreased during this stimulation period along a sigmoid time course to 4-6% of the original value. 3. In completely exhausted fibres the following membrane constants were estimated (23 degrees C): length constant, 0-31 mm; input resistance, 31 komega; membrane resistance, 58 omega.cm2. These values were calculated under the assumption of a constant internal resistivity of 170 omega. cm. The Q10 values of these constants were similar to those in normal fibres. Afew experiments revealed that the membrane capacity remained roughly constant under these conditions. 4. The current-voltage relation of exhausted fibres was approximately linear in the range between -60 and -100 mV. At less negative potentials the conductance increased slightly while at more negative potentials it decreased. The latter, in particular, became more evident when the imput current was converted into membrane current density by applying Cole's theorem. 5TEA+ and Rb+ in the external solution increased the membrane resistance of exhausted fibres by more than one order of magnitude. The major part of the membrane conductance induced by exhaustion, however, could not be blocked by these ions or Zn2+. 6. Chloride-free test solutions were used to measure the relative contributions of potassium and chloride ions to the membrane conductance. The relation GK:GC1 changed from 2:3 in normal fibres to 5:1 in exhausted ones. In absolute terms GK rose from ca. 130 to 14,300 mumho/cm2 and GC1 from ca. 200 to 2900 mumho/cm2. The discrimination between K+ and Na+ by the resting membrane in exhausted fibres was probably equal to or even higher than that under normal conditions. 7. In normal fibres the input resistance decreased by up to 20% after the external application of 1-2 mM caffeine, which is known to release calcium ions from internal stores. The elevation in internal Ca2+ by direct injection caused a small and, as a rule, irreversible decrease in input resistance which was probably partly due to local damage to the surface membrane. 8. It is concluded that in metabolically exhausted muscle fibres the surface and tubular membranes are still intact and that the observed decrease in membrane resistance is mainly due to an increase in potassium conductance. In addition, the results indicate that the gating mechanism of the potassium channels (presumably those with the characteristics of the slow component) is affected when energy reserves diminish."}
{"id": "PMID:1087933", "title": "An analysis of the influence of membrane potential and metabolic poisoning with azide on the sodium pump in skeletal muscle.", "content": "1. Activation of the Na pump in muscle by the external K concentration, [K]O, is independent of the membrane potential (Em) as shown by experiments in which Em was either stabilized during variation of [K]O or varied by application of azide at constant or zero [K]O. 2. Application of azide to Na-enriched muscles causes a  transient increase in 22Na efflux which occurs either in the presence or in the absence of external K. 3. The increased 22Na efflux induced by azide is abolished by addition of ouabain and is greatly reduced by removal of almost all of the external Na concentration, [Na]o. 4. Azide-treated muscles show a rather normal K sensitivity of 22Na efflux and [K]O induces a net Na extrusion from Na-enriched muscles in the presence of azide. 5. Azide reduces ouabain-sensitive K influx to low values thus interfering with K pump but not with the ability of K to activate the Na pump. 6. The experiments provide evidence that azide promotes a ouabainsensitive Na-Na exchange in Na-enriched muscles and that it partially uncouples the Na-K exchange normally observed.", "contents": "An analysis of the influence of membrane potential and metabolic poisoning with azide on the sodium pump in skeletal muscle. 1. Activation of the Na pump in muscle by the external K concentration, [K]O, is independent of the membrane potential (Em) as shown by experiments in which Em was either stabilized during variation of [K]O or varied by application of azide at constant or zero [K]O. 2. Application of azide to Na-enriched muscles causes a  transient increase in 22Na efflux which occurs either in the presence or in the absence of external K. 3. The increased 22Na efflux induced by azide is abolished by addition of ouabain and is greatly reduced by removal of almost all of the external Na concentration, [Na]o. 4. Azide-treated muscles show a rather normal K sensitivity of 22Na efflux and [K]O induces a net Na extrusion from Na-enriched muscles in the presence of azide. 5. Azide reduces ouabain-sensitive K influx to low values thus interfering with K pump but not with the ability of K to activate the Na pump. 6. The experiments provide evidence that azide promotes a ouabainsensitive Na-Na exchange in Na-enriched muscles and that it partially uncouples the Na-K exchange normally observed."}
{"id": "PMID:1087934", "title": "Experimental studies on the long term fate of the vein and artery grafts and its choice in aortocoronary bypass surgery.", "content": "Experimental studies of aorto-coronary bypass were performed using the technique of distal coronary artery perfusion in 47 dogs to ellucidate the flow characteristics at various grafts and long term fate of grafts up to 440 days after operation. On short term results, early graft occlusion seems to occur within 2 weeks with 6.5 days of mean in Group I and II (free grafts) when inflammatory reaction is most prominent. After 2 weeks patency rate was 65% in Group I and II, more than 80% of patency rate were obtained in Group III. In Group IV (prosthetic grafts), all but few (3 cases) could survive for more than 24 hours. In flow characteristic, artery and vein graft of which diameter is larger than that of the coronary artery can maintain enough the basal flow after ligation of the proximal coronary artery. In Group III (IMA-Circumflex anastomosis group), the internal mammary artery must be large enough to carry the blood to coronary artery lest the graft flow becomes below the basal flow. In Group IV, grafts can carry full flow but long term studies were not done because of early graft occlusion. There are much differences between free artery and vein grafts. In artery graft except IMA proper arterial structure is being kept until 440 days but has arteriosclerotic wall changes similar to human being. On the contrary, almost all free vein grafts have subintimal hyperplasia with collagen deposit and fibrous tissue and still progress after one year. These findings demonstrate that ischemia and hydrauiic effect in the graft must be important factor for the irreversible graft wall changes.", "contents": "Experimental studies on the long term fate of the vein and artery grafts and its choice in aortocoronary bypass surgery. Experimental studies of aorto-coronary bypass were performed using the technique of distal coronary artery perfusion in 47 dogs to ellucidate the flow characteristics at various grafts and long term fate of grafts up to 440 days after operation. On short term results, early graft occlusion seems to occur within 2 weeks with 6.5 days of mean in Group I and II (free grafts) when inflammatory reaction is most prominent. After 2 weeks patency rate was 65% in Group I and II, more than 80% of patency rate were obtained in Group III. In Group IV (prosthetic grafts), all but few (3 cases) could survive for more than 24 hours. In flow characteristic, artery and vein graft of which diameter is larger than that of the coronary artery can maintain enough the basal flow after ligation of the proximal coronary artery. In Group III (IMA-Circumflex anastomosis group), the internal mammary artery must be large enough to carry the blood to coronary artery lest the graft flow becomes below the basal flow. In Group IV, grafts can carry full flow but long term studies were not done because of early graft occlusion. There are much differences between free artery and vein grafts. In artery graft except IMA proper arterial structure is being kept until 440 days but has arteriosclerotic wall changes similar to human being. On the contrary, almost all free vein grafts have subintimal hyperplasia with collagen deposit and fibrous tissue and still progress after one year. These findings demonstrate that ischemia and hydrauiic effect in the graft must be important factor for the irreversible graft wall changes."}
{"id": "PMID:1087931", "title": "Rod monochromatism -- an incomplete form.", "content": "An incomplete form of rod monochromatism is described in a young man with normal visual acuity and absence of nystagmus or photophobia. ERG showed normal threshold sensitivity in white and blue lights but virtual absence of the first portion of response in red light. The sensory threshold curve lacked the typical rod-cone discontinuity, whereas the curve after full dark adaptation was normal. It is suggested that the condition is due to atypical disfunction of the cones.", "contents": "Rod monochromatism -- an incomplete form. An incomplete form of rod monochromatism is described in a young man with normal visual acuity and absence of nystagmus or photophobia. ERG showed normal threshold sensitivity in white and blue lights but virtual absence of the first portion of response in red light. The sensory threshold curve lacked the typical rod-cone discontinuity, whereas the curve after full dark adaptation was normal. It is suggested that the condition is due to atypical disfunction of the cones."}
{"id": "PMID:1087939", "title": "Transition in the character of immunological memory in mice after immunization. II. Memory in T and B cell populations.", "content": "Teh immunological memory in antibody response of mice to bovine serum albumin (BSA) was investigated at the level of antibody-producing cells or their precursor B cells and thymus-dependent helper T cells. Spleen cells obtained from mice previously primed with alum-precipitated BSA at various times were transferred to irradiated syngeneic mice. Spleen cells from mice immunized 8 days or 64 days before presented a high degree of adoptive secondary response, whereas the adoptive response of cells from mice immunized 2 days previously was found to be inferior even to that of unprimed spleen cells. Primed spleen cells treated with anti-mouse thymocyte rabbit serum plus complement were supplemented with normal thymus cells and the restoration of the responsiveness was examined. It was suggested that the memory was carried mainly by T cells in the earlier phases of the immunological memory (2 days or 8 days after the primary immunization). On the other hand, the immunological memory in the B-cell population was shown to grow gradually toward the later phase (later than 40 days).", "contents": "Transition in the character of immunological memory in mice after immunization. II. Memory in T and B cell populations. Teh immunological memory in antibody response of mice to bovine serum albumin (BSA) was investigated at the level of antibody-producing cells or their precursor B cells and thymus-dependent helper T cells. Spleen cells obtained from mice previously primed with alum-precipitated BSA at various times were transferred to irradiated syngeneic mice. Spleen cells from mice immunized 8 days or 64 days before presented a high degree of adoptive secondary response, whereas the adoptive response of cells from mice immunized 2 days previously was found to be inferior even to that of unprimed spleen cells. Primed spleen cells treated with anti-mouse thymocyte rabbit serum plus complement were supplemented with normal thymus cells and the restoration of the responsiveness was examined. It was suggested that the memory was carried mainly by T cells in the earlier phases of the immunological memory (2 days or 8 days after the primary immunization). On the other hand, the immunological memory in the B-cell population was shown to grow gradually toward the later phase (later than 40 days)."}
{"id": "PMID:1087942", "title": "[Primary hereditary band-shaped corneal dystrophy and its association with other hereditary corneal lesions (author's transl)].", "content": "The results of examinations of members of a large Tyrolian family tree with primary band-shaped corneal dystrophy and other hereditary corneal leasons are reported. Corneal lesions occurred in 16 family members. In three male members, the primary band-shaped corneal dystrophy is associated either with parenchymal opacification and endo-epithelial dystrophy or epithelial dystrophy or with parenchymal opacification and epithelial dystrophy. In some cases, various corneal dystrophies (parenchymal opacification, endo-epithelial dystrophy, cornea guttata, epithelial dystrophy, band-shaped nasal corneal dystrophy) exist without band-shaped corneal dystrophy. A polyphenic gene is responsible for the manifestation of the various corneal lesions. The occurrence of typical primary band-shaped corneal dystrophy only in male family members can be explained by sex-linked inheritance. Because of some flutuations in the expression, the primary band-shaped corneal dystrophy can only be manifested slightly on the nasal border. The hereditary character of the occurrence of the primary band-shaped corneal dystrophy and of the closely related other corneal dystrophies together with the already in 1974 observed association with keratokonus is stressed. Our observations show the variety of the manifestations of a polyphenic gene in the corneal region.", "contents": "[Primary hereditary band-shaped corneal dystrophy and its association with other hereditary corneal lesions (author's transl)]. The results of examinations of members of a large Tyrolian family tree with primary band-shaped corneal dystrophy and other hereditary corneal leasons are reported. Corneal lesions occurred in 16 family members. In three male members, the primary band-shaped corneal dystrophy is associated either with parenchymal opacification and endo-epithelial dystrophy or epithelial dystrophy or with parenchymal opacification and epithelial dystrophy. In some cases, various corneal dystrophies (parenchymal opacification, endo-epithelial dystrophy, cornea guttata, epithelial dystrophy, band-shaped nasal corneal dystrophy) exist without band-shaped corneal dystrophy. A polyphenic gene is responsible for the manifestation of the various corneal lesions. The occurrence of typical primary band-shaped corneal dystrophy only in male family members can be explained by sex-linked inheritance. Because of some flutuations in the expression, the primary band-shaped corneal dystrophy can only be manifested slightly on the nasal border. The hereditary character of the occurrence of the primary band-shaped corneal dystrophy and of the closely related other corneal dystrophies together with the already in 1974 observed association with keratokonus is stressed. Our observations show the variety of the manifestations of a polyphenic gene in the corneal region."}
{"id": "PMID:1087943", "title": "Fertilization and development of frog eggs after repeated spermiation induced by human chorionic gonadotropin.", "content": "Sperm released into the urine of adult male frogs, Rana pipiens, after intraperitoneal injection of human chorionic gonadotropin, were used to fertilize freshly ovulated eggs. Repeated spermiations from the same animal were induced with repeated injections of the hormone at intervals of four to 11 days. Individual frogs were induced to spermiate up to four times, with fertile sperm ensuing each time. Development of zygotes produced by this method did not differ from the development of ova fertilized by conventional methods.", "contents": "Fertilization and development of frog eggs after repeated spermiation induced by human chorionic gonadotropin. Sperm released into the urine of adult male frogs, Rana pipiens, after intraperitoneal injection of human chorionic gonadotropin, were used to fertilize freshly ovulated eggs. Repeated spermiations from the same animal were induced with repeated injections of the hormone at intervals of four to 11 days. Individual frogs were induced to spermiate up to four times, with fertile sperm ensuing each time. Development of zygotes produced by this method did not differ from the development of ova fertilized by conventional methods."}
{"id": "PMID:1087945", "title": "[Endoscopical findings and therapeutic decision in osteomyelosclerosis associated with portal hypertension and bleeding from esophageal varices (author's transl)].", "content": "Portal hypertension with hemorrhage from esophageal varices is a rare complication of osteomyelosclerosis; therapy is controversial. Hemostasis could be achieved in two patients by sclerozising the esophageal wall. In one of these cases hemorrhage could not be stopped on several occasions in the past by medical means; in the other patient the spleen had been removed. The patient without surgery did rather well and was in a better clinical condition than the other one; we therefore recommend to use splenectomy and splenorenal shunting in this disease rather cautiously.", "contents": "[Endoscopical findings and therapeutic decision in osteomyelosclerosis associated with portal hypertension and bleeding from esophageal varices (author's transl)]. Portal hypertension with hemorrhage from esophageal varices is a rare complication of osteomyelosclerosis; therapy is controversial. Hemostasis could be achieved in two patients by sclerozising the esophageal wall. In one of these cases hemorrhage could not be stopped on several occasions in the past by medical means; in the other patient the spleen had been removed. The patient without surgery did rather well and was in a better clinical condition than the other one; we therefore recommend to use splenectomy and splenorenal shunting in this disease rather cautiously."}
{"id": "PMID:1087944", "title": "[Vaginal flora in women from Gevgelija].", "content": "The author gives data on the vaginal flora of women aged 20--64 in the commune of Gevgelia obtained in the course of an early mass detection of the carcinoma of the uterine cervix in this area. The action covered 70.5% of women from urban areas and 85.3% of women from rural areas. The microbiological analysis of vaginal flora was made in two ways: first, by taking smears and by vital staining and classifying them after Jirovec, Peter, and Malik; another preparation was sent to the cytologist and, after fixing and staining, was interpreted microbiologically. The results of both methods are shown in tabular form, according to women's age and residence. They do not differ much between themselves. The purity of vaginal secretion in women from rural areas is higher than that in women from urban areas. The occurrence of Trichomonas vaginalis is twice as frequent in women from rural than from urban areas. There is a significant difference in the existence of D\u00f6derlein's bacillus in the postmenopausal period between women from urban and from rural parts of the commune. In women from urban areas over 60 D\u00f6derlein's bacillus was present only in 39.28% and in those from rural areas in as many as 63.07% of this group of women.", "contents": "[Vaginal flora in women from Gevgelija]. The author gives data on the vaginal flora of women aged 20--64 in the commune of Gevgelia obtained in the course of an early mass detection of the carcinoma of the uterine cervix in this area. The action covered 70.5% of women from urban areas and 85.3% of women from rural areas. The microbiological analysis of vaginal flora was made in two ways: first, by taking smears and by vital staining and classifying them after Jirovec, Peter, and Malik; another preparation was sent to the cytologist and, after fixing and staining, was interpreted microbiologically. The results of both methods are shown in tabular form, according to women's age and residence. They do not differ much between themselves. The purity of vaginal secretion in women from rural areas is higher than that in women from urban areas. The occurrence of Trichomonas vaginalis is twice as frequent in women from rural than from urban areas. There is a significant difference in the existence of D\u00f6derlein's bacillus in the postmenopausal period between women from urban and from rural parts of the commune. In women from urban areas over 60 D\u00f6derlein's bacillus was present only in 39.28% and in those from rural areas in as many as 63.07% of this group of women."}
{"id": "PMID:1087949", "title": "Transbronchial lung biopsy via the fiberoptic bronchoscope in the diagnosis of diffuse pulmonary infiltrates in the immunosuppressed host.", "content": "The differential diagnosis of diffuse pulmonary infiltrates in the immunosuppressed patient is broad. Many of the etiologies are amenable to specific therapy. Although endobronchial brush biopsy has been used to identify many infections, including Pneumocystis carinii, it was less effective in the diagnosis of noinfectious infiltrates. Transbronchial lung biopsy via the fiberoptic bronchoscope provided lung tissue that could be studied histologically, which increased the diagnostic yield without the morbidity and mortality of open or percutaneous lung biopsies.-", "contents": "Transbronchial lung biopsy via the fiberoptic bronchoscope in the diagnosis of diffuse pulmonary infiltrates in the immunosuppressed host. The differential diagnosis of diffuse pulmonary infiltrates in the immunosuppressed patient is broad. Many of the etiologies are amenable to specific therapy. Although endobronchial brush biopsy has been used to identify many infections, including Pneumocystis carinii, it was less effective in the diagnosis of noinfectious infiltrates. Transbronchial lung biopsy via the fiberoptic bronchoscope provided lung tissue that could be studied histologically, which increased the diagnostic yield without the morbidity and mortality of open or percutaneous lung biopsies.-"}
{"id": "PMID:1087950", "title": "Percutaneos pulmonary needle diagnosis of Pneumocystis carinii pneumonitis.", "content": "Percutaneous pulmonary needle aspiration (PC-A) is one of several diagnostic techniques capable of providing material in which Pneumocystis carinii organisms can be identified. This simple procedure, readily performed by most physicians, can be rapidly done under local anesthesia, requiring no sedation, general anesthetic, or multiple support personnel. When the chest roentgenographic evidence suggests diffuse involvement, PC-A may be performed at the bedside; if more patchy in distribution, under fluoroscopic guidance. Diagnostic yield approximates 80% or more on on the first attempt and approaches 100% if repeat PC-A is done after an initially negative PC-A procedure. Underlying severe bleeding disorders may lead to serious hemorrhage. Pneumothorax is not uncommon but is usually of no clinical significance and, if necessary,, is readily controlled. Percutaneous pulmonary needle biopsy with cutting or coring needles is not recommended because of a higher complication rate.", "contents": "Percutaneos pulmonary needle diagnosis of Pneumocystis carinii pneumonitis. Percutaneous pulmonary needle aspiration (PC-A) is one of several diagnostic techniques capable of providing material in which Pneumocystis carinii organisms can be identified. This simple procedure, readily performed by most physicians, can be rapidly done under local anesthesia, requiring no sedation, general anesthetic, or multiple support personnel. When the chest roentgenographic evidence suggests diffuse involvement, PC-A may be performed at the bedside; if more patchy in distribution, under fluoroscopic guidance. Diagnostic yield approximates 80% or more on on the first attempt and approaches 100% if repeat PC-A is done after an initially negative PC-A procedure. Underlying severe bleeding disorders may lead to serious hemorrhage. Pneumothorax is not uncommon but is usually of no clinical significance and, if necessary,, is readily controlled. Percutaneous pulmonary needle biopsy with cutting or coring needles is not recommended because of a higher complication rate."}
{"id": "PMID:1087951", "title": "Open lung biopsy in the diagnosis of Pneumocystis carinii pneumonia.", "content": "Lung biopsy remains the essential cornerstone in the diagnosis of pneumocystis pneumonia. Whatever technique can establish this diagnosis safely and early in order to get treatment started quickly should be used. Open lung biopsy remains a candidate for the diagnostic method of choice because if offers some advantages over percutaneous needle biopsy. There is a greater supply of competent thoracic surgeons in most hospitals than of physicians who are proficient with needle biopsy. The direct vision afforded by thoracotomy enables selective biopsy of grossly involved lung that can be appreciated by direct vision and palpation; needle biopsy is random and blind in its sampling. A more generous tissue specimen can be obtained for pathologic examination; the pathologic diagnosis of an \"unsatisfactory specimen\" is unlikely to result from open biopsy. Open lung biopsy achieves a higher diagnostic yield earlier in the pneumonitis when pneumocystis pneumonia is confined to the perihilar regions inaccessible to percutaneous needle biopsy. Better control of hemostasis is achieved by direct vision and ligature in a group of patients characteristically at high risk for bleeding tendencies, and pneumothorax is controlled in open biopsy; thoracostomy prevents the later, uncontroled collapse of the lung when the patient returns to the ward where there may be some diagnostic and therapeutic delay in the treatment of pneumothorax in a patient who can tolerate little further respiratory compromise.", "contents": "Open lung biopsy in the diagnosis of Pneumocystis carinii pneumonia. Lung biopsy remains the essential cornerstone in the diagnosis of pneumocystis pneumonia. Whatever technique can establish this diagnosis safely and early in order to get treatment started quickly should be used. Open lung biopsy remains a candidate for the diagnostic method of choice because if offers some advantages over percutaneous needle biopsy. There is a greater supply of competent thoracic surgeons in most hospitals than of physicians who are proficient with needle biopsy. The direct vision afforded by thoracotomy enables selective biopsy of grossly involved lung that can be appreciated by direct vision and palpation; needle biopsy is random and blind in its sampling. A more generous tissue specimen can be obtained for pathologic examination; the pathologic diagnosis of an \"unsatisfactory specimen\" is unlikely to result from open biopsy. Open lung biopsy achieves a higher diagnostic yield earlier in the pneumonitis when pneumocystis pneumonia is confined to the perihilar regions inaccessible to percutaneous needle biopsy. Better control of hemostasis is achieved by direct vision and ligature in a group of patients characteristically at high risk for bleeding tendencies, and pneumothorax is controlled in open biopsy; thoracostomy prevents the later, uncontroled collapse of the lung when the patient returns to the ward where there may be some diagnostic and therapeutic delay in the treatment of pneumothorax in a patient who can tolerate little further respiratory compromise."}
{"id": "PMID:1087952", "title": "Pneumocystis carinii infection: diagnosis and pathogenesis.", "content": "The methods of diagnosis of Pneumocystis carinii infection are reviewed; a high index of suspicion of the disease, particularly in the immunodeficient patient, is stressed and the classic morphology of the organism is described. Discussed are the known immunologic features gleaned from clinical and pathologic data from the literature and the reported cases from the University of Minnesota Hospitals. The importance of the complete investigation of the immune status of every patient who develops pneumocystis infection is stressed.", "contents": "Pneumocystis carinii infection: diagnosis and pathogenesis. The methods of diagnosis of Pneumocystis carinii infection are reviewed; a high index of suspicion of the disease, particularly in the immunodeficient patient, is stressed and the classic morphology of the organism is described. Discussed are the known immunologic features gleaned from clinical and pathologic data from the literature and the reported cases from the University of Minnesota Hospitals. The importance of the complete investigation of the immune status of every patient who develops pneumocystis infection is stressed."}
{"id": "PMID:1087954", "title": "Pulmonary and extrapulmonary support for patients with Pneumocystis carinii pneumonia.", "content": "Temporary extrapulmonary oxygenation may benefit selected patients with Pneumocystis carinii pneumonia who exhibit severe respiratory insufficiency. Four persons were considered candidates for extrapulmonary oxygenation with a membrane lung while under treatment for pneumocystis pneumonia. In one patient, attempts to institute membrane lung circulation were postponed until his condition was terminal. In another individual, membrane lung support was discontinued prematurely because of complications of anticoagulation. A third patient died of cardiac failure even though her oxygenation had improved during respiratory assistance. In the fourth, the membrane lung was used successfully to maintain the patient through therapy until lung recovery was adequate to resume vital function. The four cases presented are examples of the immunosuppression that creates susceptibility to pneumocystis pneumonia: In two patients, immunodeficiency was caused by lymphoma and combination chemotherapy for the underlying disease; in two others, immunosuppression was induced for the purposes of transplantation. Two patients underwent veno-venous perfusion for prepulmonary oxygenation, and one underwent venoarterial bypass with the membrane lung. Indications for, and techniques of, membrane lung bypass are reviewed. This method of extrapulmonary membrane lung support may save some patients with transient severe pulmonary insufficiency due to P, carinii pneumonia, and the membrane lung may be an adjunct to antimicrobial therapy.", "contents": "Pulmonary and extrapulmonary support for patients with Pneumocystis carinii pneumonia. Temporary extrapulmonary oxygenation may benefit selected patients with Pneumocystis carinii pneumonia who exhibit severe respiratory insufficiency. Four persons were considered candidates for extrapulmonary oxygenation with a membrane lung while under treatment for pneumocystis pneumonia. In one patient, attempts to institute membrane lung circulation were postponed until his condition was terminal. In another individual, membrane lung support was discontinued prematurely because of complications of anticoagulation. A third patient died of cardiac failure even though her oxygenation had improved during respiratory assistance. In the fourth, the membrane lung was used successfully to maintain the patient through therapy until lung recovery was adequate to resume vital function. The four cases presented are examples of the immunosuppression that creates susceptibility to pneumocystis pneumonia: In two patients, immunodeficiency was caused by lymphoma and combination chemotherapy for the underlying disease; in two others, immunosuppression was induced for the purposes of transplantation. Two patients underwent veno-venous perfusion for prepulmonary oxygenation, and one underwent venoarterial bypass with the membrane lung. Indications for, and techniques of, membrane lung bypass are reviewed. This method of extrapulmonary membrane lung support may save some patients with transient severe pulmonary insufficiency due to P, carinii pneumonia, and the membrane lung may be an adjunct to antimicrobial therapy."}
{"id": "PMID:1087955", "title": "Treatment of Pneumocystis carinii pneumonia: current status of the regimens of pentamidine isethionate and pyrimethamine-sulfadiazine.", "content": "A randomized clinical trial is under way comparing the relative merits of pentamidine isethionate and pyrimethamine-sulfadiazine in the management of patients with Pneumocystis carinii pneumonia. Twenty-one patients have entered the trial. Both regimens have produced at least one complete recovery in biopsy-proved pneumocystis pneumonia. Two patients have died from pneumocystis pneumonia after being treated with a combination of both regimens. Initial observations on comparative toxicity indicate pentamidine isethionate has more local toxicity, but pyrimethamine-sulfadiazine is difficult to administer to severely ill patients. Comparative merits of the two regimens will be analyzed at the completion of the present clinical trial.", "contents": "Treatment of Pneumocystis carinii pneumonia: current status of the regimens of pentamidine isethionate and pyrimethamine-sulfadiazine. A randomized clinical trial is under way comparing the relative merits of pentamidine isethionate and pyrimethamine-sulfadiazine in the management of patients with Pneumocystis carinii pneumonia. Twenty-one patients have entered the trial. Both regimens have produced at least one complete recovery in biopsy-proved pneumocystis pneumonia. Two patients have died from pneumocystis pneumonia after being treated with a combination of both regimens. Initial observations on comparative toxicity indicate pentamidine isethionate has more local toxicity, but pyrimethamine-sulfadiazine is difficult to administer to severely ill patients. Comparative merits of the two regimens will be analyzed at the completion of the present clinical trial."}
{"id": "PMID:1087956", "title": "Treatment of Pneumocystis carinii pneumonia in infancy.", "content": "Pentamidine is recommended for the therapy of Pneumocystis carinii pneumonia either alone or, in severe cases, combined with pyrimethamine. Attention is drawn to the importance of supporting symptomatic treatment, particularly with the promethazine-Hydergine cocktail, and to the possibilities for general and drug prophylaxis.", "contents": "Treatment of Pneumocystis carinii pneumonia in infancy. Pentamidine is recommended for the therapy of Pneumocystis carinii pneumonia either alone or, in severe cases, combined with pyrimethamine. Attention is drawn to the importance of supporting symptomatic treatment, particularly with the promethazine-Hydergine cocktail, and to the possibilities for general and drug prophylaxis."}
{"id": "PMID:1087958", "title": "Endemic infantile pneumocystis carinii infection: the Shiraz study.", "content": "Orphanage epidemics of interstitial plasma cell pneumonia (IPCP) occur among premature infants whose passive immunity due to maternally transferred antibodies has lost its effectiveness before the infants' humoral immune systems have reached sufficient maturity to respond effectively to Pneumocystis carinii. IPCP occurs also among mature newborns receiving substandard care in understaffed and crowded orphanages. Infantile diarrhea in these institutions leads to marasmus and deficient immune response in infants 3-4 months after birth. The P. carinii organisms in the alveoli elicit a plasma cell response in the interstitial tissues of the lung, which may be so massive that it interferes with respiration and leads to death by asphyxiation. Such observations at the Shiraz Orphanage are discussed.", "contents": "Endemic infantile pneumocystis carinii infection: the Shiraz study. Orphanage epidemics of interstitial plasma cell pneumonia (IPCP) occur among premature infants whose passive immunity due to maternally transferred antibodies has lost its effectiveness before the infants' humoral immune systems have reached sufficient maturity to respond effectively to Pneumocystis carinii. IPCP occurs also among mature newborns receiving substandard care in understaffed and crowded orphanages. Infantile diarrhea in these institutions leads to marasmus and deficient immune response in infants 3-4 months after birth. The P. carinii organisms in the alveoli elicit a plasma cell response in the interstitial tissues of the lung, which may be so massive that it interferes with respiration and leads to death by asphyxiation. Such observations at the Shiraz Orphanage are discussed."}
{"id": "PMID:1087959", "title": "Pneumocystis pneumonia in patients with cancer: clinical setting.", "content": "The most common clinical setting for the development of pneumocystis pneumonia in the United States is in a patient successfully treated with combination chemotherapy, usually including steroids, for a hematologic malignancy. The pneumonia is most likely to occur in the acute form during the reduction or withdrawal of chemotherapy and is the most common cause of infectious death in such cancer patients when they are in a clinical remission. The disease also develops in such patients on therapy who have uncontrolled growth of their tumor and occasionally in patients with other types of solid tumors. Given the appropriate clinical setting, all attempts should be made to establish the diagnosis histologically, but if this is not possible, empirical treatment with pentamidine isethionate is indicated.", "contents": "Pneumocystis pneumonia in patients with cancer: clinical setting. The most common clinical setting for the development of pneumocystis pneumonia in the United States is in a patient successfully treated with combination chemotherapy, usually including steroids, for a hematologic malignancy. The pneumonia is most likely to occur in the acute form during the reduction or withdrawal of chemotherapy and is the most common cause of infectious death in such cancer patients when they are in a clinical remission. The disease also develops in such patients on therapy who have uncontrolled growth of their tumor and occasionally in patients with other types of solid tumors. Given the appropriate clinical setting, all attempts should be made to establish the diagnosis histologically, but if this is not possible, empirical treatment with pentamidine isethionate is indicated."}
{"id": "PMID:1087960", "title": "Pneumocystis carinii pneumonia in the United States: epidemiologic, diagnostic, and clinical features.", "content": "Analysis of 194 patients with confirmed Pneumocystis carinii pneumonia in the United States over a 3-year period revealed that P. carinii pneumonia occurred almost exclusively in the immunosuppressed host who had a serious underlying disease. The epidemiologic features of pneumocystis pneumonia primarily reflected those of the underlying disease. P. carinii was identified antermortem in 81% of the cases usually by biopsy or needle aspiration of the lung, procedures associated with considerable morbidity and morbidity and mortality. Laboratory identification of P. carinii was usually accurate, but errors resulting from fault staining tecnique occurred. Most patients had been ill less than 2 weeks with bilateral diffuse interstitial pneumonia. Leukopenia (white blood cell count less than or equal to 3,00/mm3) and probably severe hypoxia were negative prognostic factors. Although treatment with pentamidine was effective, the drug frequently cased adverse reactions, particularly impaired renal function, when given with other nephrotoxic agents.", "contents": "Pneumocystis carinii pneumonia in the United States: epidemiologic, diagnostic, and clinical features. Analysis of 194 patients with confirmed Pneumocystis carinii pneumonia in the United States over a 3-year period revealed that P. carinii pneumonia occurred almost exclusively in the immunosuppressed host who had a serious underlying disease. The epidemiologic features of pneumocystis pneumonia primarily reflected those of the underlying disease. P. carinii was identified antermortem in 81% of the cases usually by biopsy or needle aspiration of the lung, procedures associated with considerable morbidity and morbidity and mortality. Laboratory identification of P. carinii was usually accurate, but errors resulting from fault staining tecnique occurred. Most patients had been ill less than 2 weeks with bilateral diffuse interstitial pneumonia. Leukopenia (white blood cell count less than or equal to 3,00/mm3) and probably severe hypoxia were negative prognostic factors. Although treatment with pentamidine was effective, the drug frequently cased adverse reactions, particularly impaired renal function, when given with other nephrotoxic agents."}
{"id": "PMID:1087961", "title": "Pneumocystis carinii pneumonia and primary immune deficiency diseases.", "content": "During a 3-year period, 50 cases of Pneumocystis carinii pneumonia in children less than 5 years old were reported to the Parasitic Disease Drug Service, Center for disease Control. Primary immune deficiency diseses constituted the most frequent underlying diseases in patients less than 1 year old (24/29 cases, 83%), whereas acute lymphatic leukemia was the most common underlying diseases in children 1-4 years old (17/21 cases, 81%). Severe combined immunodeficiency was the most common type of immune deficiency disease (15/25 cases, 60%). Six (24%) of the immunodeficient patients each had a sibling who died during infancy of an immunologic deficiency disease and P. carinii penumonia. Although the pathogenesis of the association between immune deficiency and P. carinii pneumonia is poorly understood, defects in both humora and cellular immunity appear to be operative.--Natl Cancer lst Monogr 43: 65-72, 1976.", "contents": "Pneumocystis carinii pneumonia and primary immune deficiency diseases. During a 3-year period, 50 cases of Pneumocystis carinii pneumonia in children less than 5 years old were reported to the Parasitic Disease Drug Service, Center for disease Control. Primary immune deficiency diseses constituted the most frequent underlying diseases in patients less than 1 year old (24/29 cases, 83%), whereas acute lymphatic leukemia was the most common underlying diseases in children 1-4 years old (17/21 cases, 81%). Severe combined immunodeficiency was the most common type of immune deficiency disease (15/25 cases, 60%). Six (24%) of the immunodeficient patients each had a sibling who died during infancy of an immunologic deficiency disease and P. carinii penumonia. Although the pathogenesis of the association between immune deficiency and P. carinii pneumonia is poorly understood, defects in both humora and cellular immunity appear to be operative.--Natl Cancer lst Monogr 43: 65-72, 1976."}
{"id": "PMID:1087962", "title": "Atypical radiographic features in Pneumocystis carinii pneumonia.", "content": "In a review of clinical, pathologic, and radiographic findings from 30 patients with confirmed pneumocystis pneumonia, the disease presented a typical radiographic picture of acute bilateral perihilar and basilar infiltrate progressing to diffuse alveolar consolidation within 3-5 days and unassociated with adenopathy or pleural changes in 13 patients. However, the incidence of atypical radiographic features was appreciable (17 of the 30 patients showed at least one atypical finding) and, in addition, few radiographic findings completely excluded the diagnosis of pneumocystis infection.", "contents": "Atypical radiographic features in Pneumocystis carinii pneumonia. In a review of clinical, pathologic, and radiographic findings from 30 patients with confirmed pneumocystis pneumonia, the disease presented a typical radiographic picture of acute bilateral perihilar and basilar infiltrate progressing to diffuse alveolar consolidation within 3-5 days and unassociated with adenopathy or pleural changes in 13 patients. However, the incidence of atypical radiographic features was appreciable (17 of the 30 patients showed at least one atypical finding) and, in addition, few radiographic findings completely excluded the diagnosis of pneumocystis infection."}
{"id": "PMID:1087963", "title": "Roentgenologic diagnosis of interstitial plasma cell pneumonia in infancy.", "content": "Sixt-two series of radiographs were analyzed. Interstitial plasma cell pneumonia (IPCP) was divided into six radiographic stages. A schematic description of these stages is given. The correlation between this scheme and the actual radiographs is illustrated with a series of nine antero-posterior and lateral radiographs from one infant. The patterns of the individual stages of the disease are then discussed with the aid of pulmonary radiographs of 12 infants and one 12-month-old child with dysgammaglobulinemia. From a prospective and retrospective analysis of these radiographs, we could delineate the development of the disease from the earliest radiologically recognizable lesions to resolution. The long incubation time of IPCP was established by a retograde analysis of several series of radiographs of proved cases; the time interval was determined between the earliest recognizable change and the full-blown picture of stage V, in which the interstitial infiltrative process may be accompained by various degrees of emphysema, edema (used synoymously with alveolar filling process), pneumothorax, and madiastinal emphysema. This scheme has proved its value during 20 years of radiographic diagnostic pactice with 30-35 annual consultations concerning IPCP; IT CORRELATES WELL WITH THE CLINICAL SYMPTOMATOLOGY AND THE RESPONSE TO THERAPY. Points of differential diagnostic importance concerning other interstitial infiltrative processes of the lungs are discussed for every stage of the disease.", "contents": "Roentgenologic diagnosis of interstitial plasma cell pneumonia in infancy. Sixt-two series of radiographs were analyzed. Interstitial plasma cell pneumonia (IPCP) was divided into six radiographic stages. A schematic description of these stages is given. The correlation between this scheme and the actual radiographs is illustrated with a series of nine antero-posterior and lateral radiographs from one infant. The patterns of the individual stages of the disease are then discussed with the aid of pulmonary radiographs of 12 infants and one 12-month-old child with dysgammaglobulinemia. From a prospective and retrospective analysis of these radiographs, we could delineate the development of the disease from the earliest radiologically recognizable lesions to resolution. The long incubation time of IPCP was established by a retograde analysis of several series of radiographs of proved cases; the time interval was determined between the earliest recognizable change and the full-blown picture of stage V, in which the interstitial infiltrative process may be accompained by various degrees of emphysema, edema (used synoymously with alveolar filling process), pneumothorax, and madiastinal emphysema. This scheme has proved its value during 20 years of radiographic diagnostic pactice with 30-35 annual consultations concerning IPCP; IT CORRELATES WELL WITH THE CLINICAL SYMPTOMATOLOGY AND THE RESPONSE TO THERAPY. Points of differential diagnostic importance concerning other interstitial infiltrative processes of the lungs are discussed for every stage of the disease."}
{"id": "PMID:1087964", "title": "[Placement of electrodes in transcutaneous stimulation for chronic pain].", "content": "Forty-six patients with chronic pain were treated with transcutaneous nerve stimulation (TNS). If there were any signs of sensory loss in the pain area, the electrodes were placed on the healthy side of the body. The effect of TNS was assessed quantitatively. After 9 months of repeated TNS, on average, the total pain score had improved by 39%. The subjective intensity of the pain, the frequency of pain and the need for analgesics had diminished by 46-47%. In those conditions in which total improvement was better than the mean (phantom limb pain, 65%; zoster neuralgia, 56%; thalamic pain, 45%), the healthy side of the body had been stimulated. In those in which the painful area had been stimulated (cancer pain, 32%; low back pain, 32%; brachialgia, 15%), the beneficial effect did not reach the mean for the whole series. This suggests that TNS of the healthy side of the body may give better long-term improvement than stimulation of the painful area. A theory of chronic pain and the mechanism of TNS is presented.", "contents": "[Placement of electrodes in transcutaneous stimulation for chronic pain]. Forty-six patients with chronic pain were treated with transcutaneous nerve stimulation (TNS). If there were any signs of sensory loss in the pain area, the electrodes were placed on the healthy side of the body. The effect of TNS was assessed quantitatively. After 9 months of repeated TNS, on average, the total pain score had improved by 39%. The subjective intensity of the pain, the frequency of pain and the need for analgesics had diminished by 46-47%. In those conditions in which total improvement was better than the mean (phantom limb pain, 65%; zoster neuralgia, 56%; thalamic pain, 45%), the healthy side of the body had been stimulated. In those in which the painful area had been stimulated (cancer pain, 32%; low back pain, 32%; brachialgia, 15%), the beneficial effect did not reach the mean for the whole series. This suggests that TNS of the healthy side of the body may give better long-term improvement than stimulation of the painful area. A theory of chronic pain and the mechanism of TNS is presented."}
{"id": "PMID:1087969", "title": "Varicose veins in New Zealand: prevalence and severity.", "content": "Three categories (none, mild, and moderate) of varicose veins are studied, and the independent variables studied are race, age, sex, height, weight. Quetelet index and parity. In Maoris the age standarised prevalence rates of varicose veins are 36.3 percent in males and 47.4 percent in females. In Pakehas the rates are 21.6 percent in males and 40.4 percent in females. In both sexes Maoris have a significantly higher prevalence of the more severe category of varicose veins. All variables studied were associated with the prevalence of varicose veins although the relationships vary by race and sex. In the univariate analyses the only variable that distinguished the three categories of varicose veins was parity in Maori females. In the multivariate analyses only in Maori males was a significant discrimination between the three groups of varicose veins achieved although \"suggestive\" discriminations were also achieved for females of both races.", "contents": "Varicose veins in New Zealand: prevalence and severity. Three categories (none, mild, and moderate) of varicose veins are studied, and the independent variables studied are race, age, sex, height, weight. Quetelet index and parity. In Maoris the age standarised prevalence rates of varicose veins are 36.3 percent in males and 47.4 percent in females. In Pakehas the rates are 21.6 percent in males and 40.4 percent in females. In both sexes Maoris have a significantly higher prevalence of the more severe category of varicose veins. All variables studied were associated with the prevalence of varicose veins although the relationships vary by race and sex. In the univariate analyses the only variable that distinguished the three categories of varicose veins was parity in Maori females. In the multivariate analyses only in Maori males was a significant discrimination between the three groups of varicose veins achieved although \"suggestive\" discriminations were also achieved for females of both races."}
{"id": "PMID:1087970", "title": "Gestational trophoblastic malignancy.", "content": "Gestational trophoblastic malignancy, although rare, offers the clinician the opportunity of being able to cure a malignant disease with cytotoxic chemotherapy. Such an opportunity is fulfilled only when the progress of the post-molar patients is monitored and chemotherapy instituted as soon as the malignant transformation of the mole is documented. The management and treatment of nine cases is presented and discussed.", "contents": "Gestational trophoblastic malignancy. Gestational trophoblastic malignancy, although rare, offers the clinician the opportunity of being able to cure a malignant disease with cytotoxic chemotherapy. Such an opportunity is fulfilled only when the progress of the post-molar patients is monitored and chemotherapy instituted as soon as the malignant transformation of the mole is documented. The management and treatment of nine cases is presented and discussed."}
{"id": "PMID:1087971", "title": "Enhanced tumor growth in chimeric mice.", "content": "Chimeras were produced by aggregation of B6D2F1 and SWA early embryos. Lewis lung tumor, syngeneic in B6D2F1, was used to study tumor growth and metastases in these chimeras. Enhanced tumor growth, low metastases rate and pronounced enlarged spleen could be observed in SWA equilibrium B6D2F1 chimeras 21 days after inoculation of tumor cells. Increased activity of suppressor T-cells which might be due to permanent allogeneic stimulation in chimeras is discussed as one of the possible mechanisms.", "contents": "Enhanced tumor growth in chimeric mice. Chimeras were produced by aggregation of B6D2F1 and SWA early embryos. Lewis lung tumor, syngeneic in B6D2F1, was used to study tumor growth and metastases in these chimeras. Enhanced tumor growth, low metastases rate and pronounced enlarged spleen could be observed in SWA equilibrium B6D2F1 chimeras 21 days after inoculation of tumor cells. Increased activity of suppressor T-cells which might be due to permanent allogeneic stimulation in chimeras is discussed as one of the possible mechanisms."}
{"id": "PMID:1087972", "title": "Aortocoronary bypass graft in dogs: late histological changes.", "content": "Late histological changes occurring in aortocoronary bypass vein grafts were studied by lignt and electron microscopy in three dogs killed one, two and three years after grafting. The changes consisted of intimal thickening due to a proliferation of modified smooth muscle cells (myointimal hyperplasia) and replacement of most of the medial smooth muscle by fibrocytes. Serial angiography in the dogs did not reveal progression of the intimal thickening after one month.", "contents": "Aortocoronary bypass graft in dogs: late histological changes. Late histological changes occurring in aortocoronary bypass vein grafts were studied by lignt and electron microscopy in three dogs killed one, two and three years after grafting. The changes consisted of intimal thickening due to a proliferation of modified smooth muscle cells (myointimal hyperplasia) and replacement of most of the medial smooth muscle by fibrocytes. Serial angiography in the dogs did not reveal progression of the intimal thickening after one month."}
{"id": "PMID:1087975", "title": "Angina pectoris after aortic valve replacement.", "content": "Angina pectoris after aortic valve replacement may be due to reduced myocardial blood flow (coronary artery stenosis or valvular dysfunction) or to increased myocardial oxygen demand (idiopathic hypertrophic subaortic stenosis or valvular dysfunction). If a patient does not do well after an aortic valve replacement, causes other than dysfunction of the prosthesis should be sought.", "contents": "Angina pectoris after aortic valve replacement. Angina pectoris after aortic valve replacement may be due to reduced myocardial blood flow (coronary artery stenosis or valvular dysfunction) or to increased myocardial oxygen demand (idiopathic hypertrophic subaortic stenosis or valvular dysfunction). If a patient does not do well after an aortic valve replacement, causes other than dysfunction of the prosthesis should be sought."}
{"id": "PMID:1087980", "title": "Chromosomal proteins in human B and T lymphocytes.", "content": "Histones and non-histone chromosomal proteins were characterized in B and T human lymphocytes by means of polyacrylamide disc gel electrophoresis. It was found that while histones do not present appreciable differences in the two examined populations, non-histone chromosomal proteins exhibit distinct electrophoretic profiles. Low molecular weight proteins predominate in B lymphocytes whereas high and intermediate proteins are largely represented in T lymphocytes. The latter proteins may be related to the capability of these resting cells to proliferate under appropriate antigenic stimuli.", "contents": "Chromosomal proteins in human B and T lymphocytes. Histones and non-histone chromosomal proteins were characterized in B and T human lymphocytes by means of polyacrylamide disc gel electrophoresis. It was found that while histones do not present appreciable differences in the two examined populations, non-histone chromosomal proteins exhibit distinct electrophoretic profiles. Low molecular weight proteins predominate in B lymphocytes whereas high and intermediate proteins are largely represented in T lymphocytes. The latter proteins may be related to the capability of these resting cells to proliferate under appropriate antigenic stimuli."}
{"id": "PMID:1087981", "title": "Isolation of kallikrein from mammary gland of cows.", "content": "Small amounts of kallikrein were isolated from bovine mammary tissue. Immediately after slaughter, the udder was removed, perfused with ice-cold xylocaine-Rheomacrodex solution, and homogenized in ice-cold acetic acid solution. Kallikrein-like substances were adsorbed on DEAE-cellulose, eluted with ammonium formate and fractionated by acetone precipitation. The 40-70% acetone fraction was separated on a Shephadex-G 75 column and aliquots of each fraction were incubated for 1 or 2 hr with kininogen. After incubation, extracts were tested for kinin activity on the isolated rat uterus, the rat duodenum, blood flow through the femoral artery of dogs and by intramammary pressure assay in sheep. Release of kinins was demonstrated by all these techniques, highest kallikrein activity being detected in the molecular weight range 50,000 to 60,000. Direct injection of the enzyme into the udder artery of sheep, without prior incubation with kinninogen, resulted in vasodilation and a rise in intramammary pressure. The kallikrein activity was strongly inhibited by aprotinin, but not by soybean trypsin inhibitor or ovomucoid. Kallikrein activity was always associated with esterase activity.", "contents": "Isolation of kallikrein from mammary gland of cows. Small amounts of kallikrein were isolated from bovine mammary tissue. Immediately after slaughter, the udder was removed, perfused with ice-cold xylocaine-Rheomacrodex solution, and homogenized in ice-cold acetic acid solution. Kallikrein-like substances were adsorbed on DEAE-cellulose, eluted with ammonium formate and fractionated by acetone precipitation. The 40-70% acetone fraction was separated on a Shephadex-G 75 column and aliquots of each fraction were incubated for 1 or 2 hr with kininogen. After incubation, extracts were tested for kinin activity on the isolated rat uterus, the rat duodenum, blood flow through the femoral artery of dogs and by intramammary pressure assay in sheep. Release of kinins was demonstrated by all these techniques, highest kallikrein activity being detected in the molecular weight range 50,000 to 60,000. Direct injection of the enzyme into the udder artery of sheep, without prior incubation with kinninogen, resulted in vasodilation and a rise in intramammary pressure. The kallikrein activity was strongly inhibited by aprotinin, but not by soybean trypsin inhibitor or ovomucoid. Kallikrein activity was always associated with esterase activity."}
{"id": "PMID:1087993", "title": "Chromosomal abnormalities in angio-immunoblastic lymphadenopathy.", "content": "Chromosomal studies have been performed in 2 patients with angio-immunoblastic lymphadenopathy. In both the cases the presence of abnormal cell lines characterized by marker chromosomes has been detected. Application of banding techniques allowed to detect the structural composition of the marker chromosome in 1 of the cases and to show a clonal evolutive pattern of the rearranged chromosomal set; In the same patient a consistent Y loss observed in the major fraction of the investigated metaphases did not appear to be related to any defined rearrangement of the karyotype. Longitudinal chromosomal studies are stressed in order to better correlate th cytogenetic abnormalities and the immunoreactive picture of the lymph nodes along the course of the disease.", "contents": "Chromosomal abnormalities in angio-immunoblastic lymphadenopathy. Chromosomal studies have been performed in 2 patients with angio-immunoblastic lymphadenopathy. In both the cases the presence of abnormal cell lines characterized by marker chromosomes has been detected. Application of banding techniques allowed to detect the structural composition of the marker chromosome in 1 of the cases and to show a clonal evolutive pattern of the rearranged chromosomal set; In the same patient a consistent Y loss observed in the major fraction of the investigated metaphases did not appear to be related to any defined rearrangement of the karyotype. Longitudinal chromosomal studies are stressed in order to better correlate th cytogenetic abnormalities and the immunoreactive picture of the lymph nodes along the course of the disease."}
{"id": "PMID:1087994", "title": "Membrane cell markers in human leukemias and lymphomas.", "content": "The availability of B- and T-cell membrane markers has provided new approaches and concepts in the study of lymphoproliferative diseases; The present status of knowledge in monoclonal B-cell proliferations, T-cell proliferations such as the S\u00e9zary syndrome and chronic lymphocytic leukemia of T-cell origin, acute lymphoblastic leukemias and non-Hodgkin lymphomas is briefly reviewed.", "contents": "Membrane cell markers in human leukemias and lymphomas. The availability of B- and T-cell membrane markers has provided new approaches and concepts in the study of lymphoproliferative diseases; The present status of knowledge in monoclonal B-cell proliferations, T-cell proliferations such as the S\u00e9zary syndrome and chronic lymphocytic leukemia of T-cell origin, acute lymphoblastic leukemias and non-Hodgkin lymphomas is briefly reviewed."}
{"id": "PMID:1087999", "title": "Studies on the ability to taste phenylthiocarbamide; red-green colour blindness and the age at menarche in Johannesburg Chinese school children.", "content": "The sample, although small, represents the total number of children attending the Johannesburg Chinese Kuo Ting Primary and High School in 1972 to 1974. The frequency of the non-taster gene was found to be 0,167 in the combined male and female sample. The gene for red-green colour blindness is present in the Johannesburg Chinese population but at low frequnecy, 0,012. The mean age at menarche was found to be 12,8+/-1,23 years and is younger than the mean ages recorded for a number of Caucasoid populations. It is suggested that this difference in mean age reflects possibly varying genetic rather than varying socio-economic factors.", "contents": "Studies on the ability to taste phenylthiocarbamide; red-green colour blindness and the age at menarche in Johannesburg Chinese school children. The sample, although small, represents the total number of children attending the Johannesburg Chinese Kuo Ting Primary and High School in 1972 to 1974. The frequency of the non-taster gene was found to be 0,167 in the combined male and female sample. The gene for red-green colour blindness is present in the Johannesburg Chinese population but at low frequnecy, 0,012. The mean age at menarche was found to be 12,8+/-1,23 years and is younger than the mean ages recorded for a number of Caucasoid populations. It is suggested that this difference in mean age reflects possibly varying genetic rather than varying socio-economic factors."}
{"id": "PMID:1088011", "title": "Defect in IgA secretion and in IgA specific suppressor cells in patients with selective IgA deficiency.", "content": "The nature of the defect in patients with selective IgA deficiency was investigated using a technique established to study terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. The peripheral blood lymphocytes from normal individuals had geometric mean synthetic rates of 4910 ng for IgM, 1625 ng for IgG and 1270 ng for IgA per 2 x 10(6) cells in culture for 7 days in the presence of pokeweed mitogen. The cultured lymphocytes from each of the 14 patients with selective IgA deficiency studied synthesized normal quantities of IgG and IgM but secreted less than 100 ng of IgA into the media. However, 11 of the 14 patients studied synthesized IgA by the 7th day in PWM stimulated cultures as assessed by staining for cytoplasmic IgA using fluorescein-labeled anti-IgA antisera. Synthesis and secretion of IgA by normal cells was not suppressed when they were co-cultured with lymphocytes from these patients that synthesize but do not secrete IgA. Three of the 14 patients did not have lymphocytes with IgA demonstrable in their cytoplasm following culture. When the lymphocytes from these 3 patients were co-cultured with normal lymphocytes and pokeweed mitogen the synthesis of IgA by the normal cells was depressed by 80 to 100%. Synthesis of IgG and IgM was not depressed. These studies suggest that lymphocytes cultured with pokeweed mitogen from the majority of patients with selective IgA deficiency can synthesize IgA but have a defect in IgA secretion. A smaller group of the patients do not synthesize IgA and have IgA specific suppressor cells that prevent B cells from maturing into IgA synthesizing and secreting cells.", "contents": "Defect in IgA secretion and in IgA specific suppressor cells in patients with selective IgA deficiency. The nature of the defect in patients with selective IgA deficiency was investigated using a technique established to study terminal differentiation of B lymphocytes into immunoglobulin synthesizing and secreting cells. The peripheral blood lymphocytes from normal individuals had geometric mean synthetic rates of 4910 ng for IgM, 1625 ng for IgG and 1270 ng for IgA per 2 x 10(6) cells in culture for 7 days in the presence of pokeweed mitogen. The cultured lymphocytes from each of the 14 patients with selective IgA deficiency studied synthesized normal quantities of IgG and IgM but secreted less than 100 ng of IgA into the media. However, 11 of the 14 patients studied synthesized IgA by the 7th day in PWM stimulated cultures as assessed by staining for cytoplasmic IgA using fluorescein-labeled anti-IgA antisera. Synthesis and secretion of IgA by normal cells was not suppressed when they were co-cultured with lymphocytes from these patients that synthesize but do not secrete IgA. Three of the 14 patients did not have lymphocytes with IgA demonstrable in their cytoplasm following culture. When the lymphocytes from these 3 patients were co-cultured with normal lymphocytes and pokeweed mitogen the synthesis of IgA by the normal cells was depressed by 80 to 100%. Synthesis of IgG and IgM was not depressed. These studies suggest that lymphocytes cultured with pokeweed mitogen from the majority of patients with selective IgA deficiency can synthesize IgA but have a defect in IgA secretion. A smaller group of the patients do not synthesize IgA and have IgA specific suppressor cells that prevent B cells from maturing into IgA synthesizing and secreting cells."}
{"id": "PMID:1088008", "title": "[Presentation of 25 cases of post-irradiation lesions of the brachial plexus and their treatment].", "content": "A study was carried out on a series of 25 patients who were operated on for breast cancer and who submitted to postoperative radiotherapy, in which lesions of the brachial nervous plexus from ionizing radiations were obtained. In almost all the cases, 24 out of 25, various symptoms such as paresthesiae, pain, and motorial disturbances, were observed even one year after the completion of the treatment. Motorial troubles, which in 13 cases were not detected at the moment of the diagnosis of the other lesions, always occurred later. Etiopathogenesis and diagnosis of these lesions have been considered, as well as the possibility of lowering the incidence of such troubles. The conclusion can be drawn that useful results can be obtained, to a certain extent, only if therapeutic treatment is performed early: in most cases, however, the lesions showed a progressive evolution.", "contents": "[Presentation of 25 cases of post-irradiation lesions of the brachial plexus and their treatment]. A study was carried out on a series of 25 patients who were operated on for breast cancer and who submitted to postoperative radiotherapy, in which lesions of the brachial nervous plexus from ionizing radiations were obtained. In almost all the cases, 24 out of 25, various symptoms such as paresthesiae, pain, and motorial disturbances, were observed even one year after the completion of the treatment. Motorial troubles, which in 13 cases were not detected at the moment of the diagnosis of the other lesions, always occurred later. Etiopathogenesis and diagnosis of these lesions have been considered, as well as the possibility of lowering the incidence of such troubles. The conclusion can be drawn that useful results can be obtained, to a certain extent, only if therapeutic treatment is performed early: in most cases, however, the lesions showed a progressive evolution."}
{"id": "PMID:1088009", "title": "Iridocorneal adhesions in posterior polymorphous dystrophy.", "content": "The variability of clinical expression in posterior polymorphous dystrophy is illustrated with emphasis on the occurrence of iridocorneal adhesions. These are believed to be synechiae and not Rieger's anomaly. The occurrence of glass-membrane-like material extending onto the iris from the cornea, causing synechiae and pupillary ectropion, is documented.", "contents": "Iridocorneal adhesions in posterior polymorphous dystrophy. The variability of clinical expression in posterior polymorphous dystrophy is illustrated with emphasis on the occurrence of iridocorneal adhesions. These are believed to be synechiae and not Rieger's anomaly. The occurrence of glass-membrane-like material extending onto the iris from the cornea, causing synechiae and pupillary ectropion, is documented."}
{"id": "PMID:1088014", "title": "[Study of cellular immunity in patients with testicular teratoblastoma by the method of rosette formation].", "content": "In 23 patients with teratoblastomas of the testes the authors studied nonspecific reactivity of T-lymphocytes by means of the reaction of spontaneous rosette-formation. A definite correlation was shown between the reaction indices, chorionic gonadotropin, the Abelev reaction and clinical course of the disease. It is concluded that the complex therapy should be conducted under the control of the corresponding immunological indices.", "contents": "[Study of cellular immunity in patients with testicular teratoblastoma by the method of rosette formation]. In 23 patients with teratoblastomas of the testes the authors studied nonspecific reactivity of T-lymphocytes by means of the reaction of spontaneous rosette-formation. A definite correlation was shown between the reaction indices, chorionic gonadotropin, the Abelev reaction and clinical course of the disease. It is concluded that the complex therapy should be conducted under the control of the corresponding immunological indices."}
{"id": "PMID:1088017", "title": "[Endoscopy and biopsy of the esophagus].", "content": "Since glass fibre instruments are used, the number of oesophagoscopies very much increased. The complication rate is small. The oesophagus can be inspected without any gaps in all sections. Our knowledge on pathogenesis and distribution of certain diseases of the oesophagus has considerably been enlarged by modern biopsy and endoscopy. In the urgent clarification of acute causes of haemorrhage in the upper alimentary tract endoscopy stands in the first place compared with all other diagnostic methods. The interpretation of the findings in the oesophagus demands an experienced and critical examinator, as the bioptic particles frequently are very small.", "contents": "[Endoscopy and biopsy of the esophagus]. Since glass fibre instruments are used, the number of oesophagoscopies very much increased. The complication rate is small. The oesophagus can be inspected without any gaps in all sections. Our knowledge on pathogenesis and distribution of certain diseases of the oesophagus has considerably been enlarged by modern biopsy and endoscopy. In the urgent clarification of acute causes of haemorrhage in the upper alimentary tract endoscopy stands in the first place compared with all other diagnostic methods. The interpretation of the findings in the oesophagus demands an experienced and critical examinator, as the bioptic particles frequently are very small."}
{"id": "PMID:1088018", "title": "[Diagnostic value of bulboscopy].", "content": "Bulboscopy is a proved routine examination in radiological suspicion of changes in the duodenum, in duodenal ulcers, haemorrhages from the superior gastrointestinal tract, also as emergency endoscopy, and in unclear epigastric complaints. The endoscopy of the duodenal bulb is better than the radiological examination, especially when a cicatricial bulb is present. Flat ulcers often cannot be proved by radiological examination. Nevertheless the radiological examination should always be carried out, as also endoscopically in the cicatricial bulb ulcers may be overseen. Endoscopically the florid duodenal ulcer can be differentiated in several groups. Some a prognostic importance is ascribed.", "contents": "[Diagnostic value of bulboscopy]. Bulboscopy is a proved routine examination in radiological suspicion of changes in the duodenum, in duodenal ulcers, haemorrhages from the superior gastrointestinal tract, also as emergency endoscopy, and in unclear epigastric complaints. The endoscopy of the duodenal bulb is better than the radiological examination, especially when a cicatricial bulb is present. Flat ulcers often cannot be proved by radiological examination. Nevertheless the radiological examination should always be carried out, as also endoscopically in the cicatricial bulb ulcers may be overseen. Endoscopically the florid duodenal ulcer can be differentiated in several groups. Some a prognostic importance is ascribed."}
{"id": "PMID:1088019", "title": "[Emergency endoscopy in upper gastrointestinal tract hemorrhages].", "content": "The urgent endoscopy of the superior gastrointestinal haemorrhage carefully and quickly helps in clarifying the following questions: Is the patient going on bleeding? Is conservative approach allowed? Must be operated? It is without danger in the hand of the experienced. In 95.20% we could get a positive evidence. In this the advantage compared with other methods is shown. The examination should possibly be done only by means of a glass fibre endoscope and may be performed only after combat against shock. It is reported on own results of 125 endoscopies in haemorrhage of the superior gastrointestinal tract.", "contents": "[Emergency endoscopy in upper gastrointestinal tract hemorrhages]. The urgent endoscopy of the superior gastrointestinal haemorrhage carefully and quickly helps in clarifying the following questions: Is the patient going on bleeding? Is conservative approach allowed? Must be operated? It is without danger in the hand of the experienced. In 95.20% we could get a positive evidence. In this the advantage compared with other methods is shown. The examination should possibly be done only by means of a glass fibre endoscope and may be performed only after combat against shock. It is reported on own results of 125 endoscopies in haemorrhage of the superior gastrointestinal tract."}
{"id": "PMID:1088020", "title": "[Hemorrhagic erosion as a cause for gastrointestinal hemorrhages].", "content": "It is necessary in clinical diagnosis more than hitherto done to take into consideration haemorrhagic erosions as the most frequent cause of gastrointestinal haemorrhages. They are found as concomitant diseases of certain basic diseases, whereby the spectre of causes deviates in adults and children. In adults dominate heart insufficiency and cerebral diseases including skull-brain trauma, in children, however, infections of the respiratory and digestive tract, followed by cerebral affections and haemoblastoses. A differentiation concerning age and sex cannot be proved.", "contents": "[Hemorrhagic erosion as a cause for gastrointestinal hemorrhages]. It is necessary in clinical diagnosis more than hitherto done to take into consideration haemorrhagic erosions as the most frequent cause of gastrointestinal haemorrhages. They are found as concomitant diseases of certain basic diseases, whereby the spectre of causes deviates in adults and children. In adults dominate heart insufficiency and cerebral diseases including skull-brain trauma, in children, however, infections of the respiratory and digestive tract, followed by cerebral affections and haemoblastoses. A differentiation concerning age and sex cannot be proved."}
{"id": "PMID:1088027", "title": "[Influence of homologous n-alkanoic acids on functional properties of isolated skeletal muscles. I. Muscle contraction].", "content": "1. The contractility of isolated muscles of the frog (and in some instances of the rat) was investigated at room temperature in Ringer's solutions containing homologous alkanoic acids (100 mM C4 to 0.4 mM C10). 2. Free fatty acids decrease the contraction amplitudes evoked by direct stimulation. The effects increase with concentration, exposure, and chain length of the fatty acids. In Ringer's solution the changes are totally or partly reversible. 3. The depression of contraction amplitude induced by free fatty acids is removed by small concentrations of caffeine (2--5 mM) in Ringer's solution. 4. Interactions of fatty acids with different structures of skeletal muscle (mitochondria, sarcolemma and membranes of sarcoplasmic vesicles) are discussed. The distinct effect of fatty acids on stimulated muscles and the importance of membranes in the regulation of the calcium ion concentration in the cytoplasm suggest that fatty acids interact with membrane lipids.", "contents": "[Influence of homologous n-alkanoic acids on functional properties of isolated skeletal muscles. I. Muscle contraction]. 1. The contractility of isolated muscles of the frog (and in some instances of the rat) was investigated at room temperature in Ringer's solutions containing homologous alkanoic acids (100 mM C4 to 0.4 mM C10). 2. Free fatty acids decrease the contraction amplitudes evoked by direct stimulation. The effects increase with concentration, exposure, and chain length of the fatty acids. In Ringer's solution the changes are totally or partly reversible. 3. The depression of contraction amplitude induced by free fatty acids is removed by small concentrations of caffeine (2--5 mM) in Ringer's solution. 4. Interactions of fatty acids with different structures of skeletal muscle (mitochondria, sarcolemma and membranes of sarcoplasmic vesicles) are discussed. The distinct effect of fatty acids on stimulated muscles and the importance of membranes in the regulation of the calcium ion concentration in the cytoplasm suggest that fatty acids interact with membrane lipids."}
{"id": "PMID:1088028", "title": "Reversal of acute myocardial ischemia in closed chest animals by retrograde perfusion of the coronary sinus with arterial blood.", "content": "The fundamental objective of this study was to develop a technique for salvaging aco the ischemic muscle segment is the coronary venous system. We have been able to ration) in closed chest dogs by placing a double lumen balloon catheter in the coronary sinus, and perfusing it with blood derived from a peripheral artery. We have been able to partially reverse these same manifestations in similar dogs with an ischemic period of 30-60 minutes.", "contents": "Reversal of acute myocardial ischemia in closed chest animals by retrograde perfusion of the coronary sinus with arterial blood. The fundamental objective of this study was to develop a technique for salvaging aco the ischemic muscle segment is the coronary venous system. We have been able to ration) in closed chest dogs by placing a double lumen balloon catheter in the coronary sinus, and perfusing it with blood derived from a peripheral artery. We have been able to partially reverse these same manifestations in similar dogs with an ischemic period of 30-60 minutes."}
{"id": "PMID:1088022", "title": "Choosing a vessel for aortocoronary bypass.", "content": "In practice, the surgeon is left with few choices of the proper ACB graft. His options are limited entirely to autologous tissues, of which the best is the great saphenous vein from the calf (Table 3). Actually, once leg veins and the internal mammary artery are no longer available there are few other choices worth considering. Small calibre allografts, however preserved, and synthetic grafts, are uniformly doomed to failure. The great saphenous vein from the calf is usually a single tube, of suitable diameter and strength for arterial replacement. Most of its tributaries lie in the upper third of the calf; this makes the lower segment preferable for the patient who requires only one or two bypasses. Variations in the normal anatomy have been illustrated.", "contents": "Choosing a vessel for aortocoronary bypass. In practice, the surgeon is left with few choices of the proper ACB graft. His options are limited entirely to autologous tissues, of which the best is the great saphenous vein from the calf (Table 3). Actually, once leg veins and the internal mammary artery are no longer available there are few other choices worth considering. Small calibre allografts, however preserved, and synthetic grafts, are uniformly doomed to failure. The great saphenous vein from the calf is usually a single tube, of suitable diameter and strength for arterial replacement. Most of its tributaries lie in the upper third of the calf; this makes the lower segment preferable for the patient who requires only one or two bypasses. Variations in the normal anatomy have been illustrated."}
{"id": "PMID:1088029", "title": "The contribution of right ventricular angiocardiography to the diagnosis of tricuspid valvular stenosis (Observations made on 3 patients).", "content": "The right ventricular angiocardiographic findings, in the anterioposterior projection, are described on three patients with tricuspid valvular stenosis. During diastole, the tricuspid valve was delineated as an arcline contour, placed between the non opacified right atrium and the opacified right ventricle, and it was displaced to the left of the spine. Its mobility was diminished. Right ventricular angiocardiography seems to be a useful method for the diagnosis of the tricuspid valvular stenosis and the estimation of the pathology and the functional condition of the tricuspid valve. The method is recommended for further evaluation.", "contents": "The contribution of right ventricular angiocardiography to the diagnosis of tricuspid valvular stenosis (Observations made on 3 patients). The right ventricular angiocardiographic findings, in the anterioposterior projection, are described on three patients with tricuspid valvular stenosis. During diastole, the tricuspid valve was delineated as an arcline contour, placed between the non opacified right atrium and the opacified right ventricle, and it was displaced to the left of the spine. Its mobility was diminished. Right ventricular angiocardiography seems to be a useful method for the diagnosis of the tricuspid valvular stenosis and the estimation of the pathology and the functional condition of the tricuspid valve. The method is recommended for further evaluation."}
{"id": "PMID:1088024", "title": "[Action potentials of the heart atria and ventricles of Rana temporaria frogs and Cyprinus carpio carp].", "content": "Using microelectrode technique, studies have been made on electrophysiological indices (amplitude of AP, amplitude of the plateau, latent period of AP, duration of maximal depolarization, duration of repolarization at different levels) of cells of isolated atrium and ventricles of the carp during both the spontaneous activity and electrical stimulation. The obtained amplitude-temporal parameters were compared to those of the heart in the frog R. temporaria. It was found that the amplitude of AP, the amplitude of the plateau and the duration of the latent period of AP in both the atrium and ventricles of the carp significantly (p less than 0.01) differ from the corresponding indices of the frog. On the contrary, the duration of maximal depolarization and repolarization in cells from homologous parts of the heart is very close in the species investigated.", "contents": "[Action potentials of the heart atria and ventricles of Rana temporaria frogs and Cyprinus carpio carp]. Using microelectrode technique, studies have been made on electrophysiological indices (amplitude of AP, amplitude of the plateau, latent period of AP, duration of maximal depolarization, duration of repolarization at different levels) of cells of isolated atrium and ventricles of the carp during both the spontaneous activity and electrical stimulation. The obtained amplitude-temporal parameters were compared to those of the heart in the frog R. temporaria. It was found that the amplitude of AP, the amplitude of the plateau and the duration of the latent period of AP in both the atrium and ventricles of the carp significantly (p less than 0.01) differ from the corresponding indices of the frog. On the contrary, the duration of maximal depolarization and repolarization in cells from homologous parts of the heart is very close in the species investigated."}
{"id": "PMID:1088023", "title": "Surgical versus medical treatment of coronary artery disease: long-term survival.", "content": "In an attempt to answer the question as to whether or not aortocoronary bypass (ACB) does increase life expectancy of patients with coronary artery occlusive disease (CAOD), 4,766 consecutive patients undergoing ACB at the Texas Heart Institute from October, 1969 through June, 1975, were reviewed and followed for five and one half years. Overall early mortality was reduced from 9.7 percent during the first full year (1970) of the study to 3.3 percent during the last full year (1974). Early mortality in males (86.5 percent) was reduced to 3 percent during 1975, but in females only to 8.4 percent. However, late mortality in females was only 2.6 percent as compared to 3.4 percent in males. Long-term survival was similar for both males and females at five and one-half years when early mortality was considered. Males also experienced better symptomatic results than females with 90.3 percent of males remaining asymptomatic, while only 86.6 percent of females remained in this category. Early mortality increased as more vessels were bypassed, but late mortality decreased and symptomatic results improved as more complete revascularization was performed. Only 55 percent of late deaths were cardiac related. Actuarial comparison of this surgical series with the most comparable series in the literature of patients treated medically, demonstrated significant (P less than 0.001) increased survival in the surgical group as compared to medically treated patients at every year up to five and one-half years, for patients with double and triple vessel disease and for the entire series. In those patients with single vessel disease, the survival curves were similar to four years, following which survival was increased in the surgical patients at the end of the fifth and sixth reporting years. In summary, these data appear to suggest that surgical treatment of coronary artery occlusive disease does provide a favorable effect upon life expectancy.", "contents": "Surgical versus medical treatment of coronary artery disease: long-term survival. In an attempt to answer the question as to whether or not aortocoronary bypass (ACB) does increase life expectancy of patients with coronary artery occlusive disease (CAOD), 4,766 consecutive patients undergoing ACB at the Texas Heart Institute from October, 1969 through June, 1975, were reviewed and followed for five and one half years. Overall early mortality was reduced from 9.7 percent during the first full year (1970) of the study to 3.3 percent during the last full year (1974). Early mortality in males (86.5 percent) was reduced to 3 percent during 1975, but in females only to 8.4 percent. However, late mortality in females was only 2.6 percent as compared to 3.4 percent in males. Long-term survival was similar for both males and females at five and one-half years when early mortality was considered. Males also experienced better symptomatic results than females with 90.3 percent of males remaining asymptomatic, while only 86.6 percent of females remained in this category. Early mortality increased as more vessels were bypassed, but late mortality decreased and symptomatic results improved as more complete revascularization was performed. Only 55 percent of late deaths were cardiac related. Actuarial comparison of this surgical series with the most comparable series in the literature of patients treated medically, demonstrated significant (P less than 0.001) increased survival in the surgical group as compared to medically treated patients at every year up to five and one-half years, for patients with double and triple vessel disease and for the entire series. In those patients with single vessel disease, the survival curves were similar to four years, following which survival was increased in the surgical patients at the end of the fifth and sixth reporting years. In summary, these data appear to suggest that surgical treatment of coronary artery occlusive disease does provide a favorable effect upon life expectancy."}
{"id": "PMID:1088030", "title": "Polycardiographic study of atrial flutter.", "content": "The modifications that atrial flutter determines on the phonocardiogram, apexcardiogram, carotid pulse tracing, jugular venous pulse tracing, and indirect (esophageal) left atrial pulse tracing were studied. On the basis of the data here presented and that of the literature, a polygraphic profile of atrial flutter has been constructed as follows: notable variability of the intensity and of the richness of the vibratory components of the first and second heart sound; regularly alternating intervals between successive atrial sounds, each one of which consists of two groups of vibrations; deformations of all mechanographic tracings corresponding with \"F\" waves of the ECG. The interpretation of various polygraphic reports contributes to the understanding of the physiopathogenesis of atrial flutter.", "contents": "Polycardiographic study of atrial flutter. The modifications that atrial flutter determines on the phonocardiogram, apexcardiogram, carotid pulse tracing, jugular venous pulse tracing, and indirect (esophageal) left atrial pulse tracing were studied. On the basis of the data here presented and that of the literature, a polygraphic profile of atrial flutter has been constructed as follows: notable variability of the intensity and of the richness of the vibratory components of the first and second heart sound; regularly alternating intervals between successive atrial sounds, each one of which consists of two groups of vibrations; deformations of all mechanographic tracings corresponding with \"F\" waves of the ECG. The interpretation of various polygraphic reports contributes to the understanding of the physiopathogenesis of atrial flutter."}
{"id": "PMID:1088031", "title": "Serum magnesium concentrations in atrial fibrillation.", "content": "Serum magnesium and digoxin levels were obtained in 13 nontoxic patients with atrial fibrillation due to chronic rheumatic heart disease receiving digoxin for the control of ventricular rate and heart failure. Fairly good correlations were made between serum digoxin levels and ventricular rates. Hypomagnesemia was quite common (7 out of 13) and mean magnesium serum levels were significantly lowered in total as well in 7 hypomagnesemic patients, as compared to in healthy controls. Magnesium sulphate was successfully used in patients with magnesium deficiency to control the ventricular rates.", "contents": "Serum magnesium concentrations in atrial fibrillation. Serum magnesium and digoxin levels were obtained in 13 nontoxic patients with atrial fibrillation due to chronic rheumatic heart disease receiving digoxin for the control of ventricular rate and heart failure. Fairly good correlations were made between serum digoxin levels and ventricular rates. Hypomagnesemia was quite common (7 out of 13) and mean magnesium serum levels were significantly lowered in total as well in 7 hypomagnesemic patients, as compared to in healthy controls. Magnesium sulphate was successfully used in patients with magnesium deficiency to control the ventricular rates."}
{"id": "PMID:1088032", "title": "Haemodynamic diversity and plasma renin activity in labile arterial hypertension.", "content": "Haemodynamic studies were made on 31 patients with labile hypertension at rest and during exercise. Plasma renin activity (PRA) was measured in 8 of them. Five haemodynamic types could be recognised and they could be arranged into two groups. The first was characterised by an increase of calculated peripheral resistance, sometimes permanent, sometimes revealed by effort, sometimes in relation to an increased cardiac output; this would appear to predict the development of permanent hypertension. The second group was characterised by normal systemic resistances, adapted to the cardiac output which was either normal or increased; the significance of labile hypertension in these cases was uncertain; from the haemodynamic studies one was unable to distinguish a transient emotive hypertension from potential permanent hypertension. The PRA was raised in the supine position and greatly increased by an orthostatic posture in the 8 patients tested, suggesting therefore an underlying neuro-adrenergic mechanism. In some patients with labile hypertension the haemodynamic tests were normal at rest and during effort. Others had different responses, which may be the result of varieties of hyper-sympathetic activity, either pure or predominantly beta-adrenergic (increased cardiac output, peripheral resistance adapted to the output) or combined beta and alpha (increased cardiac output with raised peripheral resistance) or mainly alpha-adrenergic (normal cardiac output, increased peripheral resistance).", "contents": "Haemodynamic diversity and plasma renin activity in labile arterial hypertension. Haemodynamic studies were made on 31 patients with labile hypertension at rest and during exercise. Plasma renin activity (PRA) was measured in 8 of them. Five haemodynamic types could be recognised and they could be arranged into two groups. The first was characterised by an increase of calculated peripheral resistance, sometimes permanent, sometimes revealed by effort, sometimes in relation to an increased cardiac output; this would appear to predict the development of permanent hypertension. The second group was characterised by normal systemic resistances, adapted to the cardiac output which was either normal or increased; the significance of labile hypertension in these cases was uncertain; from the haemodynamic studies one was unable to distinguish a transient emotive hypertension from potential permanent hypertension. The PRA was raised in the supine position and greatly increased by an orthostatic posture in the 8 patients tested, suggesting therefore an underlying neuro-adrenergic mechanism. In some patients with labile hypertension the haemodynamic tests were normal at rest and during effort. Others had different responses, which may be the result of varieties of hyper-sympathetic activity, either pure or predominantly beta-adrenergic (increased cardiac output, peripheral resistance adapted to the output) or combined beta and alpha (increased cardiac output with raised peripheral resistance) or mainly alpha-adrenergic (normal cardiac output, increased peripheral resistance)."}
{"id": "PMID:1088025", "title": "[Daily form of rest in the catfish Ictalurus nebulosus and the frog Rana temporaria].", "content": "By means of recording the cardiac rhythm, respiration rate and motor activity in unrestrained animals, studies have been made on the dynamics of formation of cataleptic-like immobilization in the teleost I. nebulosus. Rhythmic illumination at a rate 3/min. (10 sec light on - 10 sec light off) in the daytime gradually caused the onset of immobilization in the fish. This immobilization is characterized by the specific heart rate, type of respiration and the degree of plastic tone. Under the same experimental conditions, the formation of photogenic catalepsy in the frog Rana temporaria was confirmed. Typical heart rate, respiration pattern and the degree of plastic tone were determined as well. The role of illumination rhythm in the onset of all conditions which are characteristic for the \"awakefulness-rest\" cycle is discussed.", "contents": "[Daily form of rest in the catfish Ictalurus nebulosus and the frog Rana temporaria]. By means of recording the cardiac rhythm, respiration rate and motor activity in unrestrained animals, studies have been made on the dynamics of formation of cataleptic-like immobilization in the teleost I. nebulosus. Rhythmic illumination at a rate 3/min. (10 sec light on - 10 sec light off) in the daytime gradually caused the onset of immobilization in the fish. This immobilization is characterized by the specific heart rate, type of respiration and the degree of plastic tone. Under the same experimental conditions, the formation of photogenic catalepsy in the frog Rana temporaria was confirmed. Typical heart rate, respiration pattern and the degree of plastic tone were determined as well. The role of illumination rhythm in the onset of all conditions which are characteristic for the \"awakefulness-rest\" cycle is discussed."}
{"id": "PMID:1088033", "title": "Late spontaneous closure of a large ventricular septal defect.", "content": "The authors report a case of a large ventricular septal defect with pulmonary hypertension, cardiomegaly and heart failure in early infancy, exhibiting marked improvement at five years of age. Subsequent followup, revealed spontaneous closure of the defect demonstrated by hemodynamic studies between 7 and 8 years of age, at which time the patient became completely asymptomatic with disappearance of all abnormal physical findings.", "contents": "Late spontaneous closure of a large ventricular septal defect. The authors report a case of a large ventricular septal defect with pulmonary hypertension, cardiomegaly and heart failure in early infancy, exhibiting marked improvement at five years of age. Subsequent followup, revealed spontaneous closure of the defect demonstrated by hemodynamic studies between 7 and 8 years of age, at which time the patient became completely asymptomatic with disappearance of all abnormal physical findings."}
{"id": "PMID:1088034", "title": "Double atrial parasystole. Case report.", "content": "A 62 year-old man with hiatal hernia and complex atrial arrhythmia is reported. On the standard electrocardiogram a double atrial parasystole is found, with reversed coupling. During carotid sinus massage only one of the parasystolic foci is not influenced, and assumes the atrial activity, regularizing the cardiac rhythm.", "contents": "Double atrial parasystole. Case report. A 62 year-old man with hiatal hernia and complex atrial arrhythmia is reported. On the standard electrocardiogram a double atrial parasystole is found, with reversed coupling. During carotid sinus massage only one of the parasystolic foci is not influenced, and assumes the atrial activity, regularizing the cardiac rhythm."}
{"id": "PMID:1088035", "title": "Severe coarctation with similar pressure at four limbs. Clinical, catheterization, angiographic and surgical data of one case.", "content": "In patient with severe coarctation of the aorta, arterial pressures were similar at the four limbs due to departure of both subclavian arteries from the descending aorta. The clinical clues to the diagnosis and the hemodynamic, angiocardiographic and surgical data are presented.", "contents": "Severe coarctation with similar pressure at four limbs. Clinical, catheterization, angiographic and surgical data of one case. In patient with severe coarctation of the aorta, arterial pressures were similar at the four limbs due to departure of both subclavian arteries from the descending aorta. The clinical clues to the diagnosis and the hemodynamic, angiocardiographic and surgical data are presented."}
{"id": "PMID:1088036", "title": "Arthritis and heart lesions. A study of 25 cases with pericarditis or valvular lesions associated to inflammatory joint disease.", "content": "Clinical and laboratory data of 25 patients with inflammatory joint disease and pericarditis or valvular heart disease are reviewed. The patients were divided in two groups: 11 presented pericarditis and 14 valvular heart disease. The patients studied presented a spectrum of diseases ranging from classical sero-positive rheumatoid arthritis to ankylosing spondylitis. Acute rheumatic fever was in no case the actual joint disease. In contrast to the patients with valvular heart disease nearly all patients with pericarditis were rheumatoid arthritis factor positive and signs of a generalized systemic disease with vasculitis were also more frequent. In the pericarditis group there was no sex difference in contrast to the valvular group where females were more often affected. The heart lesions were usually detected late in the course of the chronic joint disease. Valvular heart disease occurs not only in ankylosing spondylitis but also in rheumatoid arthritis, usually the sero-negative type. In the light of a survey of the literature, the pertinent findings are discussed.", "contents": "Arthritis and heart lesions. A study of 25 cases with pericarditis or valvular lesions associated to inflammatory joint disease. Clinical and laboratory data of 25 patients with inflammatory joint disease and pericarditis or valvular heart disease are reviewed. The patients were divided in two groups: 11 presented pericarditis and 14 valvular heart disease. The patients studied presented a spectrum of diseases ranging from classical sero-positive rheumatoid arthritis to ankylosing spondylitis. Acute rheumatic fever was in no case the actual joint disease. In contrast to the patients with valvular heart disease nearly all patients with pericarditis were rheumatoid arthritis factor positive and signs of a generalized systemic disease with vasculitis were also more frequent. In the pericarditis group there was no sex difference in contrast to the valvular group where females were more often affected. The heart lesions were usually detected late in the course of the chronic joint disease. Valvular heart disease occurs not only in ankylosing spondylitis but also in rheumatoid arthritis, usually the sero-negative type. In the light of a survey of the literature, the pertinent findings are discussed."}
{"id": "PMID:1088037", "title": "[Immediate and long-term results of surgery of the tricuspid valve. APropos of 62 operated cases].", "content": "Operative and late results in a series of patients operated on the tricuspid valve (replacement or annuloplasty) at the moment of surgery upon mitral and aortic valves are reported. Functional tricuspid insufficiency carries a greater risk than organic tricuspid disease. Considering the duration of the underlying mitral disease, four grades are set up for the mitro-tricuspid patient allowing for prognosis and indication of the type of surgery to be done on the tricuspid valve.", "contents": "[Immediate and long-term results of surgery of the tricuspid valve. APropos of 62 operated cases]. Operative and late results in a series of patients operated on the tricuspid valve (replacement or annuloplasty) at the moment of surgery upon mitral and aortic valves are reported. Functional tricuspid insufficiency carries a greater risk than organic tricuspid disease. Considering the duration of the underlying mitral disease, four grades are set up for the mitro-tricuspid patient allowing for prognosis and indication of the type of surgery to be done on the tricuspid valve."}
{"id": "PMID:1088038", "title": "The angiocardiographic appearance of the endocardial cushion defect in selected transposition and malposition complexes.", "content": "Although the angiocardiographic features of the endocardial cushion defect (ECD) have been well described in the patient with two normally related ventricles (D-ventricular loop in situs solitus) and normally related great arteries, little attention has been focused on the angiocardiographic appearance of the ECD in patients with complex cardiovascular disease. Because of recent surgical advances in the treatment of single ventricle and double outlet right ventricle complexes, it has become increasingly important to document the status of the atrioventricular valves in these patients. The angiocardiographic features of the ECD are therefore described in two patients, one with D-loop, single left ventricle and outlet chamber, D-transposition of the great arteries, bilateral conus and pulmonary stenosis and the other with dextrocardia, situs inversus, double outlet right ventricle, common atrioventricular canal and pulmonary stenosis. In both patients, the left ventricular outflow tract, despite aortic-mitral discontinuity, is characterized by elongation, scalloping and serration, and the classic appearance of the \"gooseneck\" deformity is observed on left ventricular angiography. It thus appears that aortic-mitral continuity and two well-formed, normally related ventricles and great arteries are not invariable necessary to the manifestation of the \"gooseneck\" deformity in the patient with complex cardiovascular disease and associated endocardial cushion defect.", "contents": "The angiocardiographic appearance of the endocardial cushion defect in selected transposition and malposition complexes. Although the angiocardiographic features of the endocardial cushion defect (ECD) have been well described in the patient with two normally related ventricles (D-ventricular loop in situs solitus) and normally related great arteries, little attention has been focused on the angiocardiographic appearance of the ECD in patients with complex cardiovascular disease. Because of recent surgical advances in the treatment of single ventricle and double outlet right ventricle complexes, it has become increasingly important to document the status of the atrioventricular valves in these patients. The angiocardiographic features of the ECD are therefore described in two patients, one with D-loop, single left ventricle and outlet chamber, D-transposition of the great arteries, bilateral conus and pulmonary stenosis and the other with dextrocardia, situs inversus, double outlet right ventricle, common atrioventricular canal and pulmonary stenosis. In both patients, the left ventricular outflow tract, despite aortic-mitral discontinuity, is characterized by elongation, scalloping and serration, and the classic appearance of the \"gooseneck\" deformity is observed on left ventricular angiography. It thus appears that aortic-mitral continuity and two well-formed, normally related ventricles and great arteries are not invariable necessary to the manifestation of the \"gooseneck\" deformity in the patient with complex cardiovascular disease and associated endocardial cushion defect."}
{"id": "PMID:1088039", "title": "The electromechanical interval in normal subjects and after myocardial infarction.", "content": "The electromechanical interval (EMI) was measured in 25 normal males, 56 male patients with a myocardial infarction and 6 patients with a ventricular aneurysm. In the normal subjects the EMI was found to be unrelated to the heart rate but the isovolumic contraction time was inversely related to the heart rate. The EMI was increased after myocardial infarction but this increase did not occur in patientswho had a non-transmural infarction when they had not had any previous myocardial infarction. The increase was more marked in the patients with a ventricular aneurysm. It is suggested that the prolongation of EMI is due to areas of abnormal left ventricular wall movement and that this effect may be a useful aid to the diagnosis of ventricular aneurysm.", "contents": "The electromechanical interval in normal subjects and after myocardial infarction. The electromechanical interval (EMI) was measured in 25 normal males, 56 male patients with a myocardial infarction and 6 patients with a ventricular aneurysm. In the normal subjects the EMI was found to be unrelated to the heart rate but the isovolumic contraction time was inversely related to the heart rate. The EMI was increased after myocardial infarction but this increase did not occur in patientswho had a non-transmural infarction when they had not had any previous myocardial infarction. The increase was more marked in the patients with a ventricular aneurysm. It is suggested that the prolongation of EMI is due to areas of abnormal left ventricular wall movement and that this effect may be a useful aid to the diagnosis of ventricular aneurysm."}
{"id": "PMID:1088040", "title": "Haemodynamic alterations of the left ventricle during right atrial pacing.", "content": "The left ventricular haemodynamic alterations during right atrial pacing were studied in 12 cases. Cardiac index varied little: during maximal rate however, its mean value was slightly lower than the resting one. Stroke index decreased inversely to the heart rate. The course of these indices did not separate the normal from abnormal cases. Ventricular function curves (VFCs) were constructed by relating the changes of left ventricular (LV) end-diastolic pressure (EDP) to those of stroke index (SI). In 4 normal cases the curves were steep, showing a fall of EDP with relatively large decrease of SI; in 3 cases of congestive myocardiopathy they were flat, showing fall of EDP in two and increase in one, with relatively small decrease of SI; in 5 patients with effort angina LVEDP initially decreased. This initial fall of VFCs was steep in two with normal and flattened in three with impaired resting LV function. Increase of EDP, evidently due to development of ischaemia, followed in all; it exceeded resting EDP in two out of three cases developing angina and in one out of two not developing angina. Our findings support the view that the increase of LVEDP is due to decrease of both myocardial contractility and compliance.", "contents": "Haemodynamic alterations of the left ventricle during right atrial pacing. The left ventricular haemodynamic alterations during right atrial pacing were studied in 12 cases. Cardiac index varied little: during maximal rate however, its mean value was slightly lower than the resting one. Stroke index decreased inversely to the heart rate. The course of these indices did not separate the normal from abnormal cases. Ventricular function curves (VFCs) were constructed by relating the changes of left ventricular (LV) end-diastolic pressure (EDP) to those of stroke index (SI). In 4 normal cases the curves were steep, showing a fall of EDP with relatively large decrease of SI; in 3 cases of congestive myocardiopathy they were flat, showing fall of EDP in two and increase in one, with relatively small decrease of SI; in 5 patients with effort angina LVEDP initially decreased. This initial fall of VFCs was steep in two with normal and flattened in three with impaired resting LV function. Increase of EDP, evidently due to development of ischaemia, followed in all; it exceeded resting EDP in two out of three cases developing angina and in one out of two not developing angina. Our findings support the view that the increase of LVEDP is due to decrease of both myocardial contractility and compliance."}
{"id": "PMID:1088041", "title": "Anomalous coronary arterial pattern.", "content": "The clinical history of an adult patient with anginal-like pain is described. A coronary angiography revealed the existence of a hypoplastic left coronary artery, a right coronary artery which passed around the posterior surface of the heart and continued into the anterior surface of the heart to give off an anterior descending artery and the presence of a \"conus\" artery. The embryonic genesis of hypoplastic coronary artery and of a similar aberration, single coronary artery, are discussed.", "contents": "Anomalous coronary arterial pattern. The clinical history of an adult patient with anginal-like pain is described. A coronary angiography revealed the existence of a hypoplastic left coronary artery, a right coronary artery which passed around the posterior surface of the heart and continued into the anterior surface of the heart to give off an anterior descending artery and the presence of a \"conus\" artery. The embryonic genesis of hypoplastic coronary artery and of a similar aberration, single coronary artery, are discussed."}
{"id": "PMID:1088042", "title": "Multiple coronary arteriovenous fistulae.", "content": "A case with multiple congenital coronary arteriovenous fistulae is reported. The right coronary artery was communicating with the right ventricle. The left coronary artery was entering directly into the pulmonary trunk and two terminal branches of the anterior descending into the left ventricle. The direct communication of the left coronary artery without interposition of an accessory artery or a circoid plexus is met for the first time.", "contents": "Multiple coronary arteriovenous fistulae. A case with multiple congenital coronary arteriovenous fistulae is reported. The right coronary artery was communicating with the right ventricle. The left coronary artery was entering directly into the pulmonary trunk and two terminal branches of the anterior descending into the left ventricle. The direct communication of the left coronary artery without interposition of an accessory artery or a circoid plexus is met for the first time."}
{"id": "PMID:1088043", "title": "Pseudo-retrograde conduction in complete A-V heart block due to ectopic His bundle activity.", "content": "A case with complete A-V heart block in whom retrograde P- waves following some of the ventricular complexes were due to an ectopic junctional activity with the characteristics of parasystole is reported. His bundle electrogram showed an infrahisian block. After administration of 1 mg atropine intravenously the retrograde P- waves were detached from the ventricular automatic complexes and an H' deflection preceded each inverted P- wave with an H'-A' interval of 40 msec.", "contents": "Pseudo-retrograde conduction in complete A-V heart block due to ectopic His bundle activity. A case with complete A-V heart block in whom retrograde P- waves following some of the ventricular complexes were due to an ectopic junctional activity with the characteristics of parasystole is reported. His bundle electrogram showed an infrahisian block. After administration of 1 mg atropine intravenously the retrograde P- waves were detached from the ventricular automatic complexes and an H' deflection preceded each inverted P- wave with an H'-A' interval of 40 msec."}
{"id": "PMID:1088044", "title": "Prenylamine inhibition of isoproterenol induced myocardial lesions. Histochemical and ultrastructural findings.", "content": "Eighty Wistar rats were divided into 4 groups. All of them received 10 mg/kg i.p. isoproterenol (ISP). The animals were sacrificed at 5 min. (groups A and B) and 24 hours (groups C and D). Groups A and C served as control of group B and D. Group B also received 9 mg/kg prenylamine (P), 30 min before ISP and group D 9 mg/kg P, 1 hour before and 1 hour after ISP. Transversal slices of each heart underwent the following procedures: hematoxilineosin, Barbeito-Lopez trichromic, \"ischemia stain\", acid phosphatase stain and standard electron microscopy. Group A and C showed positivity for ischemic techniques; necrotic and ischemic zones represented, in average 25.1 +/- 1.4% of the myocardial volume. Group B and D (ISP + prenylamine) showed no significant lesions. Group D (ISP + prenylamine, sacrificed at 24 hours) presented complete absence of \"infarct-like\" lesions in 17 out of the 20 studied animals. No evident changes in acid-phosphatase enzymes were detected. Animals treated with ISP alone presented \"contraction bands\" at electron microscopy as well as myofibrillar fragmentation. Those receiving also P showed only light alterations, in the electron microscopy specimens. A primary action of ISP on the calcium pump might explain the infarct-like lesions found in our study. The inhibition of these above mentioned lesions by prenylamine, a drug which acts by slowing down the ca transport, also supports this interpretation.", "contents": "Prenylamine inhibition of isoproterenol induced myocardial lesions. Histochemical and ultrastructural findings. Eighty Wistar rats were divided into 4 groups. All of them received 10 mg/kg i.p. isoproterenol (ISP). The animals were sacrificed at 5 min. (groups A and B) and 24 hours (groups C and D). Groups A and C served as control of group B and D. Group B also received 9 mg/kg prenylamine (P), 30 min before ISP and group D 9 mg/kg P, 1 hour before and 1 hour after ISP. Transversal slices of each heart underwent the following procedures: hematoxilineosin, Barbeito-Lopez trichromic, \"ischemia stain\", acid phosphatase stain and standard electron microscopy. Group A and C showed positivity for ischemic techniques; necrotic and ischemic zones represented, in average 25.1 +/- 1.4% of the myocardial volume. Group B and D (ISP + prenylamine) showed no significant lesions. Group D (ISP + prenylamine, sacrificed at 24 hours) presented complete absence of \"infarct-like\" lesions in 17 out of the 20 studied animals. No evident changes in acid-phosphatase enzymes were detected. Animals treated with ISP alone presented \"contraction bands\" at electron microscopy as well as myofibrillar fragmentation. Those receiving also P showed only light alterations, in the electron microscopy specimens. A primary action of ISP on the calcium pump might explain the infarct-like lesions found in our study. The inhibition of these above mentioned lesions by prenylamine, a drug which acts by slowing down the ca transport, also supports this interpretation."}
{"id": "PMID:1088045", "title": "Mechanical alterations of the left ventricle during right atrial pacing.", "content": "The effect of increasing heart rate by right atrial pacing on the peak value of the first derivative of left ventricular (LV) pressure(dp/dt) and the maximal velocity of the contractile element (KVmax) was studied in 12 cases. Peak dp/dt was poor as regards its sensitivity in reflecting the changes of contractility, due to its strong dependence on LV end-diastolic (EDP) and systolic pressure. KVmax increased constantly in the 4 normals and in 2 cases of ischaemic heart disease which did not develop angina; the increase exceeded 90 ml sec-1 in the former and one of the latter cases in which resting LV function was normal. In contrast, it decreased during the development of ischaemia in two of the three cases which developed angina; in the third case, in which also resting LV function was seriously impaired, the course of KVmax was almost flat. A similar flat course was observed in the three cases of congestive myocardiopathy. the above alterations of KV max were independent of the EDP and proportional to the basic contractility and its anticipated changes during pacing.", "contents": "Mechanical alterations of the left ventricle during right atrial pacing. The effect of increasing heart rate by right atrial pacing on the peak value of the first derivative of left ventricular (LV) pressure(dp/dt) and the maximal velocity of the contractile element (KVmax) was studied in 12 cases. Peak dp/dt was poor as regards its sensitivity in reflecting the changes of contractility, due to its strong dependence on LV end-diastolic (EDP) and systolic pressure. KVmax increased constantly in the 4 normals and in 2 cases of ischaemic heart disease which did not develop angina; the increase exceeded 90 ml sec-1 in the former and one of the latter cases in which resting LV function was normal. In contrast, it decreased during the development of ischaemia in two of the three cases which developed angina; in the third case, in which also resting LV function was seriously impaired, the course of KVmax was almost flat. A similar flat course was observed in the three cases of congestive myocardiopathy. the above alterations of KV max were independent of the EDP and proportional to the basic contractility and its anticipated changes during pacing."}
{"id": "PMID:1088046", "title": "[Electrophysiologic study of fenoxedil chlorhydrate].", "content": "Electrophysiologic studies were carried out on 10 patients before and during therapy by Fenoxedil Chlorhydrate a new anti-arrhythmic drug, to determine the effects on cardiac conduction. Fenoxedil Chlorhydrate produces a constant alteration of intra-nodal conduction. Modification of sinus function are less evident. Auricular and ventricular refractory periods are increased in the majority of cases. These results give a better understanding of mecanism of this drug, and helps in its prescription in clinical practice.", "contents": "[Electrophysiologic study of fenoxedil chlorhydrate]. Electrophysiologic studies were carried out on 10 patients before and during therapy by Fenoxedil Chlorhydrate a new anti-arrhythmic drug, to determine the effects on cardiac conduction. Fenoxedil Chlorhydrate produces a constant alteration of intra-nodal conduction. Modification of sinus function are less evident. Auricular and ventricular refractory periods are increased in the majority of cases. These results give a better understanding of mecanism of this drug, and helps in its prescription in clinical practice."}
{"id": "PMID:1088047", "title": "Magnesium and the heart.", "content": "Magnesium is a most important cation in the body mostly distributed in the skeleton, muscles and liver. The most important causes of magnesium deficiency in the body include wide range of gastrointestinal disorders, diuretic therapy, endocrine disorders, renal diseases, alcoholism, etc. The demonstration of hypomagnesemia in patients of digitalis toxicity is of great clinical significance since magnesium can be replaced among these patients and toxicity and mortality due to this drug can be minimised. In patients with low serum magnesium, the cellular content of this ion may be normal. Therefore, it is of great interest to study the myocardial level of this ion in cardiovascular diseases. The clinical features of magnesium deficit are related to neuromuscular disorders. However, ST and T waves changes are quite commonly seen in the electrocardiogram and cardiac arrhythmias rarely have also been described. Magnesium administration on the other hand causes hypotention, bradycardia, and conduction defects. Magnesium depresses central nervous system, neuromuscular transmission and conductivity of the heart. Magnesium therapy is indicated when clinical features of magnesium deficiency are present in association with low serum or tissue levels.", "contents": "Magnesium and the heart. Magnesium is a most important cation in the body mostly distributed in the skeleton, muscles and liver. The most important causes of magnesium deficiency in the body include wide range of gastrointestinal disorders, diuretic therapy, endocrine disorders, renal diseases, alcoholism, etc. The demonstration of hypomagnesemia in patients of digitalis toxicity is of great clinical significance since magnesium can be replaced among these patients and toxicity and mortality due to this drug can be minimised. In patients with low serum magnesium, the cellular content of this ion may be normal. Therefore, it is of great interest to study the myocardial level of this ion in cardiovascular diseases. The clinical features of magnesium deficit are related to neuromuscular disorders. However, ST and T waves changes are quite commonly seen in the electrocardiogram and cardiac arrhythmias rarely have also been described. Magnesium administration on the other hand causes hypotention, bradycardia, and conduction defects. Magnesium depresses central nervous system, neuromuscular transmission and conductivity of the heart. Magnesium therapy is indicated when clinical features of magnesium deficiency are present in association with low serum or tissue levels."}
{"id": "PMID:1088050", "title": "Left ventricular aneurysmectomy for refractory tachyarrhythmias: report in 11 postinfarction patients.", "content": "Between January 1970 and August 1974, eleven postinfarction patients underwent left ventricular aneurysmectomy for refractory tachyarrhythmias. Ten suffered from ventricular tachycardias and one from supraventricular tachycardia. All had localized aneurysms and normal contractions of the surrounding myocardium on angiocardiography. None suffered from significant angina, congestive heart failure or papillary muscle dysfunction. The diagnosis was first suspected at fluoroscopy, the latter being in our opinion a most reliable screening method for the recognition of these cases. There was one hospital death after surgery. The remaining ten patients are now free from further arrhythmic attacks for a follow-up period between fourteen months to five and a half years. These results confirm that in some carefully selected postinfarction patients, left ventricular aneurysmectomy without concomittant aortocoronary bypass may result in the elimination of medically intractable tachyarrhythmias.", "contents": "Left ventricular aneurysmectomy for refractory tachyarrhythmias: report in 11 postinfarction patients. Between January 1970 and August 1974, eleven postinfarction patients underwent left ventricular aneurysmectomy for refractory tachyarrhythmias. Ten suffered from ventricular tachycardias and one from supraventricular tachycardia. All had localized aneurysms and normal contractions of the surrounding myocardium on angiocardiography. None suffered from significant angina, congestive heart failure or papillary muscle dysfunction. The diagnosis was first suspected at fluoroscopy, the latter being in our opinion a most reliable screening method for the recognition of these cases. There was one hospital death after surgery. The remaining ten patients are now free from further arrhythmic attacks for a follow-up period between fourteen months to five and a half years. These results confirm that in some carefully selected postinfarction patients, left ventricular aneurysmectomy without concomittant aortocoronary bypass may result in the elimination of medically intractable tachyarrhythmias."}
{"id": "PMID:1088051", "title": "Prognostic value of intraventricular conduction blocks in acute myocardial infarction.", "content": "An intraventricular conduction defect complicated 61 out of 765 cases of acute myocardial infarctions. The defect, with the exception of two cases, always developed in infarctions affecting the anterior wall of the heart. Compared with the other acute anterior myocardial infarctions in which the mortality in the Coronary Care Unit was 15%, infarctions complicated by intraventricular conduction defects have on the whole, presented a worse clinical course and a higher mortality rate (54%). It must be stressed that clinical course and mortality rate have been very different in the various conduction defects. The most innocuous conduction defects were the anterior fascicular block, and, with some reserve, the right bundle branch block too, defects in which the clinical course and the mortality rate were not significantly different from those of the other anterior myocardial infarctions not complicated by intraventricular conduction troubles. On the contrary clinical course was more serious and mortality rate higher (52%) in the cases in which the two defects were associated. Myocardial infarctions complicated by left bundle branch block, right bundle branch block with posterior fascicular block, alternating bundle branch block presented an extremely serious clinical course and a very high mortality rate (over 85%). These differences in the clinical course and in the mortality rate of the various intraventricular conduction defects are explained by the fact that they are associated with myocardial damage of different degree, which is clarified by knowledge of the blood supply of the intraventricular conduction system.", "contents": "Prognostic value of intraventricular conduction blocks in acute myocardial infarction. An intraventricular conduction defect complicated 61 out of 765 cases of acute myocardial infarctions. The defect, with the exception of two cases, always developed in infarctions affecting the anterior wall of the heart. Compared with the other acute anterior myocardial infarctions in which the mortality in the Coronary Care Unit was 15%, infarctions complicated by intraventricular conduction defects have on the whole, presented a worse clinical course and a higher mortality rate (54%). It must be stressed that clinical course and mortality rate have been very different in the various conduction defects. The most innocuous conduction defects were the anterior fascicular block, and, with some reserve, the right bundle branch block too, defects in which the clinical course and the mortality rate were not significantly different from those of the other anterior myocardial infarctions not complicated by intraventricular conduction troubles. On the contrary clinical course was more serious and mortality rate higher (52%) in the cases in which the two defects were associated. Myocardial infarctions complicated by left bundle branch block, right bundle branch block with posterior fascicular block, alternating bundle branch block presented an extremely serious clinical course and a very high mortality rate (over 85%). These differences in the clinical course and in the mortality rate of the various intraventricular conduction defects are explained by the fact that they are associated with myocardial damage of different degree, which is clarified by knowledge of the blood supply of the intraventricular conduction system."}
{"id": "PMID:1088052", "title": "Surface maps and myocardial scanning at rest and during exercise: comparison with coronary angiography.", "content": "The observations of surface electromaps recorded at rest and during exercise in two examplative cases are reported: the first one deals with a normal man while the second refers to a patient suffering from an old inferior myocardial infarction with bouts of angina pectoris; these surface maps are compared with myocardial scanning also performed at rest and during exercise. Special attention is devoted to the possible relation of the above described, non-invasive, techniques to the anatomical description of coronary lesions as demonstrated by coronary angiography. A close relationship is found between the surface potential distribution pattern and the area of hypofixation observed on the scan as far as the presumed location of ischemic myocardial tissue is concerned.", "contents": "Surface maps and myocardial scanning at rest and during exercise: comparison with coronary angiography. The observations of surface electromaps recorded at rest and during exercise in two examplative cases are reported: the first one deals with a normal man while the second refers to a patient suffering from an old inferior myocardial infarction with bouts of angina pectoris; these surface maps are compared with myocardial scanning also performed at rest and during exercise. Special attention is devoted to the possible relation of the above described, non-invasive, techniques to the anatomical description of coronary lesions as demonstrated by coronary angiography. A close relationship is found between the surface potential distribution pattern and the area of hypofixation observed on the scan as far as the presumed location of ischemic myocardial tissue is concerned."}
{"id": "PMID:1088053", "title": "Polysplenia with left ventricular hypoplasia and partial anomalous pulmonary venous connection.", "content": "A case is reported of left ventricular hypoplasia associated with anisosplenia. Even though congenital heart malformations are frequently found in cases of splenic anomalies, the association of polysplenia with hypoplastic left heart syndrome is extremely rare. The existence in addition of partial anomalous pulmonary venous connection of the right pulmonary veins to the right atrium, gave us the possibility of study the different changes in pulmonary arteries wall thickness in both lungs.", "contents": "Polysplenia with left ventricular hypoplasia and partial anomalous pulmonary venous connection. A case is reported of left ventricular hypoplasia associated with anisosplenia. Even though congenital heart malformations are frequently found in cases of splenic anomalies, the association of polysplenia with hypoplastic left heart syndrome is extremely rare. The existence in addition of partial anomalous pulmonary venous connection of the right pulmonary veins to the right atrium, gave us the possibility of study the different changes in pulmonary arteries wall thickness in both lungs."}
{"id": "PMID:1088054", "title": "Prinzmetal's variant angina.", "content": "Two patients are described with a typical Prinzmetal's variant angina. Both patients were young, active males with angina of recent onset. They experienced angina only at rest and in one patient the angina had a cyclic character appearing only between 4 and 10 a.m. In both patients there was no correlation whatsoever between the angina pectoris and effort, emotion or change in temperature and the angina reacted promptly to nitroglycerine sublingually. The number of attacks and the intensity of the pain were increasing rapidly over a short period of time in both cases. The stenoses in both patients did not exceed 50% of the lumen of the coronary arteries, but coronary surgery has been performed on clinical grounds because both patients were completely disabled and one of them suffered from life-threatening dysrhythmias. For respectively 18 and 6 months after saphenous vein bypass surgery both patients have been symptom-free and able to resume their work. The problems concerning the diagnosis Prinzmetal's variant of angina pectoris are discussed.", "contents": "Prinzmetal's variant angina. Two patients are described with a typical Prinzmetal's variant angina. Both patients were young, active males with angina of recent onset. They experienced angina only at rest and in one patient the angina had a cyclic character appearing only between 4 and 10 a.m. In both patients there was no correlation whatsoever between the angina pectoris and effort, emotion or change in temperature and the angina reacted promptly to nitroglycerine sublingually. The number of attacks and the intensity of the pain were increasing rapidly over a short period of time in both cases. The stenoses in both patients did not exceed 50% of the lumen of the coronary arteries, but coronary surgery has been performed on clinical grounds because both patients were completely disabled and one of them suffered from life-threatening dysrhythmias. For respectively 18 and 6 months after saphenous vein bypass surgery both patients have been symptom-free and able to resume their work. The problems concerning the diagnosis Prinzmetal's variant of angina pectoris are discussed."}
{"id": "PMID:1088055", "title": "[Hepatic lesions and coagulation disorders in pulmonary embolism: a clinico-pathological case].", "content": "The authors described the case of a 49-patient suffering from obstructive broncho-pneumopathy and ischemic myocardiopathy complicated by multiple pulmonary embolism. The clinics were dominated by signs of major hepatic deficiency and of intravascular disseminated coagulation with fibrinolysis. The clinical and biological parameters evoking pulmonary embolism in that intricated chart were discussed.", "contents": "[Hepatic lesions and coagulation disorders in pulmonary embolism: a clinico-pathological case]. The authors described the case of a 49-patient suffering from obstructive broncho-pneumopathy and ischemic myocardiopathy complicated by multiple pulmonary embolism. The clinics were dominated by signs of major hepatic deficiency and of intravascular disseminated coagulation with fibrinolysis. The clinical and biological parameters evoking pulmonary embolism in that intricated chart were discussed."}
{"id": "PMID:1088057", "title": "[X-ray documentation of the anamnesis and physical data in patients with rheumatism within the scope of the medical information system WAMIS].", "content": "A computerized on-line system of evaluating of symptoms and physical data in rheumatology is presented. Case history, previous treatment, and physical examination are documented. The documentation of the latter may be performed in two ways: one records only joint symptoms, the other includes joint measurements. In comparison to the former off-line method, all data are much better available. Moreover, we believe it to be considerably timesaving. This system should also allow an electronic differential diagnosis of rheumatic diseases, after completing laboratry and X-ray documentation.", "contents": "[X-ray documentation of the anamnesis and physical data in patients with rheumatism within the scope of the medical information system WAMIS]. A computerized on-line system of evaluating of symptoms and physical data in rheumatology is presented. Case history, previous treatment, and physical examination are documented. The documentation of the latter may be performed in two ways: one records only joint symptoms, the other includes joint measurements. In comparison to the former off-line method, all data are much better available. Moreover, we believe it to be considerably timesaving. This system should also allow an electronic differential diagnosis of rheumatic diseases, after completing laboratry and X-ray documentation."}
{"id": "PMID:1088058", "title": "[Effect of distraction by various forces on the elbow joint in rabbits].", "content": "The elbow joints of 115 adult live rabbits have been subjected to experimental distraction by various forces and for different intervals. The forces applied were one tenth and one half, respectively, of the mean body weights; thus, a calculated reduction of one eleventh and five elevenths, respectively, of the force transmitted through the articulating joint surfaces was achieved. The distraction was performed for periods of 1, 2, 4 and 8 weeks. The forces were administered by means of an extraarticular device which allowed motion of the joints. Controls had been provided for to prove the noneffectiveness of the distraction device per se to the joint structures under study. Both upper limbs--one side always served as control--were subjected to radiological, histological/histochemical, angiographic and cytological examination; cartilage in addition was treated by vital staining or, alternatively, by producing splitting lines. The results were as follows: (1) Extensive local and more generalized hyperemia involving the whole limb in question was produced regardless of strength and duration of distraction. (2) Under all experimental conditions subsynovial hemorrhage and hemarthrosis developed. (3) Subsynovial hemorrhage caused the development of granulomatous and, subsequently, fibrous synovitis with simultaneously demonstrable hemosiderin depots. (4) Hemarthrosis was persistent during the entire period of observation. (5) Articular cartilage revealed moderate signs of degeneration and also irregularities of the usual pattern of splitting lines after continuous distraction for 8 weeks. At the same time, subchondral bone atrophy appeared. Vital staining revealed increased cartilage staining under mild distraction; moderate and marked distraction caused definitively less staining. Subsynovial hemorrhage and hemarthrosis are explained as partial vacuum effects. Concerning the moderate cartilage changes, a multifactorial genesis is presumed; the possible role of chemical, nutritional and mechanical factors is discussed. The widespread hyperemia most likely is caused by mechanical stimulation going along with joint distraction; capsular hyperemia might be a phenomenon immediately related to synovitis. Finally, the experimental results are discussed relative to observations made during clinical traction therapy.", "contents": "[Effect of distraction by various forces on the elbow joint in rabbits]. The elbow joints of 115 adult live rabbits have been subjected to experimental distraction by various forces and for different intervals. The forces applied were one tenth and one half, respectively, of the mean body weights; thus, a calculated reduction of one eleventh and five elevenths, respectively, of the force transmitted through the articulating joint surfaces was achieved. The distraction was performed for periods of 1, 2, 4 and 8 weeks. The forces were administered by means of an extraarticular device which allowed motion of the joints. Controls had been provided for to prove the noneffectiveness of the distraction device per se to the joint structures under study. Both upper limbs--one side always served as control--were subjected to radiological, histological/histochemical, angiographic and cytological examination; cartilage in addition was treated by vital staining or, alternatively, by producing splitting lines. The results were as follows: (1) Extensive local and more generalized hyperemia involving the whole limb in question was produced regardless of strength and duration of distraction. (2) Under all experimental conditions subsynovial hemorrhage and hemarthrosis developed. (3) Subsynovial hemorrhage caused the development of granulomatous and, subsequently, fibrous synovitis with simultaneously demonstrable hemosiderin depots. (4) Hemarthrosis was persistent during the entire period of observation. (5) Articular cartilage revealed moderate signs of degeneration and also irregularities of the usual pattern of splitting lines after continuous distraction for 8 weeks. At the same time, subchondral bone atrophy appeared. Vital staining revealed increased cartilage staining under mild distraction; moderate and marked distraction caused definitively less staining. Subsynovial hemorrhage and hemarthrosis are explained as partial vacuum effects. Concerning the moderate cartilage changes, a multifactorial genesis is presumed; the possible role of chemical, nutritional and mechanical factors is discussed. The widespread hyperemia most likely is caused by mechanical stimulation going along with joint distraction; capsular hyperemia might be a phenomenon immediately related to synovitis. Finally, the experimental results are discussed relative to observations made during clinical traction therapy."}
{"id": "PMID:1088061", "title": "[Complete alpha-1-antitrypsin deficiency in a patient with Ehlers-Danlos syndrome].", "content": "Complete alpha-1-antitrypsin deficiency of the type PiOO associated with chronic obstructive lung disease, cutaneous hyperextensibility and hyperlaxity of joints were found in a nineteen-year-old Moroccan boy. On a nosological point of view, this patient could be included as a 8th form, in the Ehlers-Danlos syndrome which now groups seven clinical variants. A causal relationship between the biological deficiency and the clinical alterations (pulmonary, cutaneous and articular) could be assumed according to the biological and ultrastructural findings.", "contents": "[Complete alpha-1-antitrypsin deficiency in a patient with Ehlers-Danlos syndrome]. Complete alpha-1-antitrypsin deficiency of the type PiOO associated with chronic obstructive lung disease, cutaneous hyperextensibility and hyperlaxity of joints were found in a nineteen-year-old Moroccan boy. On a nosological point of view, this patient could be included as a 8th form, in the Ehlers-Danlos syndrome which now groups seven clinical variants. A causal relationship between the biological deficiency and the clinical alterations (pulmonary, cutaneous and articular) could be assumed according to the biological and ultrastructural findings."}
{"id": "PMID:1088063", "title": "The response of alpha-motoneurons of different size to stretch and vibration of extensor muscles after injection of 5-hydroxytryptophan in spinal cats.", "content": "1. Vibration of the GS muscle at 100/sec, and the peak-to-peak amplitude of about 100 mu, produced slight reflex activity of homonymous and heteronymous alpha-motoneurons in the spinal, unanesthetized cat. 2. Intravenous injection of the 5-HT precursor 5-HTP (25 mg/kg, L-form) performed in the spinal preparation facilitated the responses of single extensor alpha-motoneurons to both vibration and stretch of the corresponding muscle. The 5-HT antagonist methysergide (0-5 mg/kg i. v.) blocked almost completely the facilitation of the vibration and stretch induced motoneuronal discharges produced by 5-HTP in the spinal cat, indicating that the facilitation was 5-HT specific. 4. A comparison of the types of alpha-motoneurons participating in the reflex responses elicited by the different stimuli in the spinal cat following injection of 5-HTP showed that both small-tonic and large-phasic alpha-motoneurons take part in both stretch and vibration reflexes. 5. It is concluded that the alpha-motoneuron pool of the GS muscle represents an homogenous population, no matter what kind of synaptic drive is used for its activation, and that the susceptibility of a motoneuron to discharge depends on the amount of the afferent excitatory input rather than on the modalities of sensory stimulation.", "contents": "The response of alpha-motoneurons of different size to stretch and vibration of extensor muscles after injection of 5-hydroxytryptophan in spinal cats. 1. Vibration of the GS muscle at 100/sec, and the peak-to-peak amplitude of about 100 mu, produced slight reflex activity of homonymous and heteronymous alpha-motoneurons in the spinal, unanesthetized cat. 2. Intravenous injection of the 5-HT precursor 5-HTP (25 mg/kg, L-form) performed in the spinal preparation facilitated the responses of single extensor alpha-motoneurons to both vibration and stretch of the corresponding muscle. The 5-HT antagonist methysergide (0-5 mg/kg i. v.) blocked almost completely the facilitation of the vibration and stretch induced motoneuronal discharges produced by 5-HTP in the spinal cat, indicating that the facilitation was 5-HT specific. 4. A comparison of the types of alpha-motoneurons participating in the reflex responses elicited by the different stimuli in the spinal cat following injection of 5-HTP showed that both small-tonic and large-phasic alpha-motoneurons take part in both stretch and vibration reflexes. 5. It is concluded that the alpha-motoneuron pool of the GS muscle represents an homogenous population, no matter what kind of synaptic drive is used for its activation, and that the susceptibility of a motoneuron to discharge depends on the amount of the afferent excitatory input rather than on the modalities of sensory stimulation."}
{"id": "PMID:1088064", "title": "Preparation and testing of goat anti--thymocyte globulin.", "content": "This study details methods of production, purification and testing of large volumes of uniformly potent, non-toxic, anti-human thymocyte globulin (ATG). Plasma from 70 goats immunised with human thymic lymphocytes was fractionated by ammonium sulphate precipitation to its IgG component. Contaminating antibodies were removed by absorption methods. After fractionation and absorption, ATG retained its ability to inhibit the formation of rosettes by human lymphocytes with sheep red blood cells. Antibodies to human glomerular basement membrane were not detected in any tested preparation. Tests performed on monkeys, mice and rabbits revealed low toxicity.", "contents": "Preparation and testing of goat anti--thymocyte globulin. This study details methods of production, purification and testing of large volumes of uniformly potent, non-toxic, anti-human thymocyte globulin (ATG). Plasma from 70 goats immunised with human thymic lymphocytes was fractionated by ammonium sulphate precipitation to its IgG component. Contaminating antibodies were removed by absorption methods. After fractionation and absorption, ATG retained its ability to inhibit the formation of rosettes by human lymphocytes with sheep red blood cells. Antibodies to human glomerular basement membrane were not detected in any tested preparation. Tests performed on monkeys, mice and rabbits revealed low toxicity."}
{"id": "PMID:1088065", "title": "[Experimental studies on the possibility of influencing mast cells in experimental carrageenin-induced rat paw edema. Histological study on the effects of some non-steroidal anti-inflammatory agents (author's transl)].", "content": "The protective effect on mast cells of some non-steroid antiphlogistics in the carrageenin-induced edema of the rat hind paw was studied with histological methods. The number of the mast cells was counted in the inflamed connective tissue of unpretreated animals and also such pretreated with acetylsalicylacid, phenylbutazone and indometacin and the results of the test periods were compared with one another. The number of mast cells in the inflamed connective tissue of unpretreated animals was reduced in comparison to the controls during the first 5-35 min after inflammation. Six hours after inflammation the number of mast cells increased. Our investigations demonstrated that the used antiphlogistics produced a significant reduction of mast cell degeneration during the first 5-70 min after inflammation. The results are discussed.", "contents": "[Experimental studies on the possibility of influencing mast cells in experimental carrageenin-induced rat paw edema. Histological study on the effects of some non-steroidal anti-inflammatory agents (author's transl)]. The protective effect on mast cells of some non-steroid antiphlogistics in the carrageenin-induced edema of the rat hind paw was studied with histological methods. The number of the mast cells was counted in the inflamed connective tissue of unpretreated animals and also such pretreated with acetylsalicylacid, phenylbutazone and indometacin and the results of the test periods were compared with one another. The number of mast cells in the inflamed connective tissue of unpretreated animals was reduced in comparison to the controls during the first 5-35 min after inflammation. Six hours after inflammation the number of mast cells increased. Our investigations demonstrated that the used antiphlogistics produced a significant reduction of mast cell degeneration during the first 5-70 min after inflammation. The results are discussed."}
{"id": "PMID:1088062", "title": "[Activity inhibiting leukocyte migration in human sera of healthy subjects and of subjects with delayed hypersensitivity and findings of possible serum factors with antagonistic activity (author's transl)].", "content": "The AA. have tested human sera from subjects with a clinical condition of delayed hypersensitivity (TBC and eczematous contact dermatitis) in order to detect factors affecting leukocyte migration in agarose plates. They found either inhibiting or stimulating factors of cellular migration. According to the most recent data about the same area they emphasyze a sort a regulatory mechanism of LIF or factors with activity LIF-like, due to antagonistic substances present in sera of their patients. Furthermore they suggest a list of factors able to inhibit or to enhance cellular migration.", "contents": "[Activity inhibiting leukocyte migration in human sera of healthy subjects and of subjects with delayed hypersensitivity and findings of possible serum factors with antagonistic activity (author's transl)]. The AA. have tested human sera from subjects with a clinical condition of delayed hypersensitivity (TBC and eczematous contact dermatitis) in order to detect factors affecting leukocyte migration in agarose plates. They found either inhibiting or stimulating factors of cellular migration. According to the most recent data about the same area they emphasyze a sort a regulatory mechanism of LIF or factors with activity LIF-like, due to antagonistic substances present in sera of their patients. Furthermore they suggest a list of factors able to inhibit or to enhance cellular migration."}
{"id": "PMID:1088071", "title": "Aspecific transfer factor in children with Hodgkin's disease.", "content": "Six children suffering from Hodgkin's disease (HD), in different stages and free from chemo- and radiotherapy from at least four weeks, were treated with transfer factor (TF). PPD skin test, PHA-responsiveness, E-rosettes, B-lymphocytes were checked before TF therapy, after 6 and 9 TF doses and compared to the data at the onset of the disease. Two children with HD who did not receive TF, were examined as controls. PPD skin tests, PHA-responsiveness, E-rosettes did not ameliorate following TF treatment, while it has been noticed an increase in B-lymphocytes, both in percentage and absolute number. The Authors conclude that TF might have induced a slight B lymphoproliferation.", "contents": "Aspecific transfer factor in children with Hodgkin's disease. Six children suffering from Hodgkin's disease (HD), in different stages and free from chemo- and radiotherapy from at least four weeks, were treated with transfer factor (TF). PPD skin test, PHA-responsiveness, E-rosettes, B-lymphocytes were checked before TF therapy, after 6 and 9 TF doses and compared to the data at the onset of the disease. Two children with HD who did not receive TF, were examined as controls. PPD skin tests, PHA-responsiveness, E-rosettes did not ameliorate following TF treatment, while it has been noticed an increase in B-lymphocytes, both in percentage and absolute number. The Authors conclude that TF might have induced a slight B lymphoproliferation."}
{"id": "PMID:1088066", "title": "[Interposition meso-caval shunt for haemorrhage or ascites in hepatic cirrhosis. Report of twenty-eight cases (author's transl)].", "content": "The results are set out of emergency or electiver surgery for bleeding oesophageal carices or for intractable ascites in hepatic cirrhosis. The procedure adopted, a meso-caval shunt with prothesis interposition, led to a low percentage of thrombosis, markedly lowered portal pressure, and gave effective clinical results. Although angiography indicated that the portal flow to the liver had dropped considerably, or even ceased altogether, the bad effect on liver function and the incidence of encephalopathy seemed reduced than generally reported after porta-cava shunts. No deaths occurred among the patients for who surgery had been planned, but the rate was still high in emergency cases. This indicates that although well tolerated, the interposition meso-cava shunt is best performed after careful pre-operative selection and preparation.", "contents": "[Interposition meso-caval shunt for haemorrhage or ascites in hepatic cirrhosis. Report of twenty-eight cases (author's transl)]. The results are set out of emergency or electiver surgery for bleeding oesophageal carices or for intractable ascites in hepatic cirrhosis. The procedure adopted, a meso-caval shunt with prothesis interposition, led to a low percentage of thrombosis, markedly lowered portal pressure, and gave effective clinical results. Although angiography indicated that the portal flow to the liver had dropped considerably, or even ceased altogether, the bad effect on liver function and the incidence of encephalopathy seemed reduced than generally reported after porta-cava shunts. No deaths occurred among the patients for who surgery had been planned, but the rate was still high in emergency cases. This indicates that although well tolerated, the interposition meso-cava shunt is best performed after careful pre-operative selection and preparation."}
{"id": "PMID:1088072", "title": "[Receptors for murine red blood cells on normal lymphocytes and in chronic lymphocytic leukemia].", "content": "The ability of unfractionated and purified B and T peripheral lymphocytes to form rosettes EAC and E with sheep and mouse red blood cells was assessed in thirty healthy controls and in five patients with chronic lymphocytic leukaemia. E mouse rosettes is a marker of B cells in man and its percentage in peripheral blood lymphocytes is 9.3% in normal subjects on the contrary patients with CLL had a high rosette count with mouse red blood cells (mean 57%) and a low count with sheep red blood cells (mean 13.7%).", "contents": "[Receptors for murine red blood cells on normal lymphocytes and in chronic lymphocytic leukemia]. The ability of unfractionated and purified B and T peripheral lymphocytes to form rosettes EAC and E with sheep and mouse red blood cells was assessed in thirty healthy controls and in five patients with chronic lymphocytic leukaemia. E mouse rosettes is a marker of B cells in man and its percentage in peripheral blood lymphocytes is 9.3% in normal subjects on the contrary patients with CLL had a high rosette count with mouse red blood cells (mean 57%) and a low count with sheep red blood cells (mean 13.7%)."}
{"id": "PMID:1088073", "title": "Alfa-antitrypsin increase in lung cancer.", "content": "The Authors controlled the content of A1AT in the sera of 100 patients with L.Ca. As controls there were examined: 20 cases of metastatic neoplasm of the lung, 5 cases of systemic sarcomatosis, 10 cases of acute bacterial bronchopneumonia, 4 cases of bronchiectasis and emphysema. Moreover, the levels of A1AT was studied in 15 pl.fl., neoplastic and not, in comparison with the levels of the sera. None of the controls demonstrated an increase in A1AT, excepting the cases of acute bronchopneumonia, in which the levels were very high but only for a few days. The results indicate that in more than 90% of the L.Ca. the level of the A1AT is significantly higher than in the sera of the controls, with possible variations during the disease. There is a clear trend towards increase parallel with the worsening of the condition of the patients. A course of chemotherapy may bring down the levels, proportionally to the length and intensity of the treatment. After discontinuance, the level increases again more or less quickly, reaching the previous values.", "contents": "Alfa-antitrypsin increase in lung cancer. The Authors controlled the content of A1AT in the sera of 100 patients with L.Ca. As controls there were examined: 20 cases of metastatic neoplasm of the lung, 5 cases of systemic sarcomatosis, 10 cases of acute bacterial bronchopneumonia, 4 cases of bronchiectasis and emphysema. Moreover, the levels of A1AT was studied in 15 pl.fl., neoplastic and not, in comparison with the levels of the sera. None of the controls demonstrated an increase in A1AT, excepting the cases of acute bronchopneumonia, in which the levels were very high but only for a few days. The results indicate that in more than 90% of the L.Ca. the level of the A1AT is significantly higher than in the sera of the controls, with possible variations during the disease. There is a clear trend towards increase parallel with the worsening of the condition of the patients. A course of chemotherapy may bring down the levels, proportionally to the length and intensity of the treatment. After discontinuance, the level increases again more or less quickly, reaching the previous values."}
{"id": "PMID:1088067", "title": "[Present position of non-shunt surgery for bleeding oesophageal varices (author's transl)].", "content": "This is a critical retrospective study of a homogeneous group of 24 patients with hepatic cirrhosis and portal hypertension who underwent emergency surgery for bleeding oesophageal varices. The adopted procedure was the porta-azygos disconnection, according to Torres-Degni, with or without splenectomy. The criterion derived from the need to stop bleeding, from contraindications to a portal-systemic shunt, or from other situations which are illustrated. The difficulty of selecting in these cases the appropriate surgery and the high operative death rate (46%) is commented. In survivors, the long-term outcome depends on the progress of the underlying liver disease.", "contents": "[Present position of non-shunt surgery for bleeding oesophageal varices (author's transl)]. This is a critical retrospective study of a homogeneous group of 24 patients with hepatic cirrhosis and portal hypertension who underwent emergency surgery for bleeding oesophageal varices. The adopted procedure was the porta-azygos disconnection, according to Torres-Degni, with or without splenectomy. The criterion derived from the need to stop bleeding, from contraindications to a portal-systemic shunt, or from other situations which are illustrated. The difficulty of selecting in these cases the appropriate surgery and the high operative death rate (46%) is commented. In survivors, the long-term outcome depends on the progress of the underlying liver disease."}
{"id": "PMID:1088074", "title": "[Behavior of hemagglutination-inhibiting antibodies against measles and rubella in a group of children with chronic hepatitis].", "content": "The Authors studied the amount of anti-measles and anti-rubella heteroantibodies during chronic hepatitis and the possible role of these antibodies in eliciting and maintaining the hypergammaglobulinemic picture, characteristic of this morbid condition. The results they obtained in an almost exclusively pediatric case-list (37 cases) show, whenever the contact with these viruses (measles and rubella) occurred, a percentage of subjects with comparatively higher levels among chronic liver patients in comparison with control patients, of the same age. The percentage of subjects with previous rubella infection and hemagglutination-inhibiting antibody levels higher than 1:256 is about 45% among chronic liver patients, compared with 13% in healthy subjects. As far as measles virus is concerned the percentage drops from 11% to 4%. The most reliable hypotheses to explain this interesting phenomenon, which, however, does explain only partially, and concerning some cases only, the entity of the hypergammaglobulineamia, are set forth briefly.", "contents": "[Behavior of hemagglutination-inhibiting antibodies against measles and rubella in a group of children with chronic hepatitis]. The Authors studied the amount of anti-measles and anti-rubella heteroantibodies during chronic hepatitis and the possible role of these antibodies in eliciting and maintaining the hypergammaglobulinemic picture, characteristic of this morbid condition. The results they obtained in an almost exclusively pediatric case-list (37 cases) show, whenever the contact with these viruses (measles and rubella) occurred, a percentage of subjects with comparatively higher levels among chronic liver patients in comparison with control patients, of the same age. The percentage of subjects with previous rubella infection and hemagglutination-inhibiting antibody levels higher than 1:256 is about 45% among chronic liver patients, compared with 13% in healthy subjects. As far as measles virus is concerned the percentage drops from 11% to 4%. The most reliable hypotheses to explain this interesting phenomenon, which, however, does explain only partially, and concerning some cases only, the entity of the hypergammaglobulineamia, are set forth briefly."}
{"id": "PMID:1088069", "title": "[Further comments on changes in hepatic function after interposition meso-caval shunt (author's transl)].", "content": "Further to a previous paper, the Authors followed up ten cirrhotic patients who had all undergone meso-caval shunt with Dacron prosthesis, for ascites or haemorrhage, Functional and anzymatic investigations were carried on over periods up to 10 months. No constant changes in hepatic function parameters were found during the post-operative period, compared to those before surgery. This observation suggests that these investigations might be more useful in defining liver disease than in practical assessment of transient, post-operative liver failure.", "contents": "[Further comments on changes in hepatic function after interposition meso-caval shunt (author's transl)]. Further to a previous paper, the Authors followed up ten cirrhotic patients who had all undergone meso-caval shunt with Dacron prosthesis, for ascites or haemorrhage, Functional and anzymatic investigations were carried on over periods up to 10 months. No constant changes in hepatic function parameters were found during the post-operative period, compared to those before surgery. This observation suggests that these investigations might be more useful in defining liver disease than in practical assessment of transient, post-operative liver failure."}
{"id": "PMID:1088075", "title": "[Studies on various parameters of the T-dependent lymphocyte system in the course of osteosarcoma].", "content": "Some parameters of the T-lymphocyte dependent system have been studied in 20 patients with osteosarcoma. The functional condition of T lymphocytes, stimulated with mitogens (PHA and Con-A) has been investigated by evaluating DNA neosynthesis by means of the incorporation of the tritiated thymidine in lymphocyte cultures. The percentage of rosette-forming T l-mphocytes with sheep erythrocytes has been assessed. The findings would indicate a constant inhibition of DNA biosynthesis in patients with osteosarcoma, only in correspondence of PHA stimulation. This finding would agree with E rosette pattern. In this case also neoplastic patients do show, in comparison with the controls, a constant drop of the number of T lymphocytes forming rosettes with sheep erythrocytes.", "contents": "[Studies on various parameters of the T-dependent lymphocyte system in the course of osteosarcoma]. Some parameters of the T-lymphocyte dependent system have been studied in 20 patients with osteosarcoma. The functional condition of T lymphocytes, stimulated with mitogens (PHA and Con-A) has been investigated by evaluating DNA neosynthesis by means of the incorporation of the tritiated thymidine in lymphocyte cultures. The percentage of rosette-forming T l-mphocytes with sheep erythrocytes has been assessed. The findings would indicate a constant inhibition of DNA biosynthesis in patients with osteosarcoma, only in correspondence of PHA stimulation. This finding would agree with E rosette pattern. In this case also neoplastic patients do show, in comparison with the controls, a constant drop of the number of T lymphocytes forming rosettes with sheep erythrocytes."}
{"id": "PMID:1088068", "title": "[Dissection-ligation of the oesophagus according to Vosschulte for bleeding varices (author's transl)].", "content": "Ligation of the oesophagus on a Vossschulte prosthesis provides interruption of the venous back-flow in gastro-oesophageal wall occurring in portal hypertension, and therefore renders more effective the disconnection techniques used for oesophageal varices when liver function is too severely impaired to permit a portal-systemic shunt. According the literature and their own initial experience, the Authors stresse the possibilities of the method in controlling active haemorrhage and preventing further bleeding, and discusse its possible complications, represented by portal thrombosis, fistulae and oesophageal stenosis. Unlike shunts, this procedure does not give rise to any worsening in hepatic function, but should nevertheless seriously considered especially if the oesophago-gastric junction is radically isolated.", "contents": "[Dissection-ligation of the oesophagus according to Vosschulte for bleeding varices (author's transl)]. Ligation of the oesophagus on a Vossschulte prosthesis provides interruption of the venous back-flow in gastro-oesophageal wall occurring in portal hypertension, and therefore renders more effective the disconnection techniques used for oesophageal varices when liver function is too severely impaired to permit a portal-systemic shunt. According the literature and their own initial experience, the Authors stresse the possibilities of the method in controlling active haemorrhage and preventing further bleeding, and discusse its possible complications, represented by portal thrombosis, fistulae and oesophageal stenosis. Unlike shunts, this procedure does not give rise to any worsening in hepatic function, but should nevertheless seriously considered especially if the oesophago-gastric junction is radically isolated."}
{"id": "PMID:1088076", "title": "[Recent studies concerning the effect of phytohemagglutinins (PHA) on the rosette E formation from the part of peripheral human lymphocytes].", "content": "In a previous paper it was demonstrated that the treatment of human peripheral lymphocytes with phytohaemagglutinin increases their ability to form E-rosettes, probably inducing rosette formation also by B-lymphocytes. In this paper new data are reported concerning the machanism of this phenomenon.", "contents": "[Recent studies concerning the effect of phytohemagglutinins (PHA) on the rosette E formation from the part of peripheral human lymphocytes]. In a previous paper it was demonstrated that the treatment of human peripheral lymphocytes with phytohaemagglutinin increases their ability to form E-rosettes, probably inducing rosette formation also by B-lymphocytes. In this paper new data are reported concerning the machanism of this phenomenon."}
{"id": "PMID:1088070", "title": "[Postoperative gastrointestinal hemorrhages after pulmonary resections (author's transly].", "content": "Postoperative gastrointestinal hemorrhages are a infrequent complication of pulmonary resections. Nevertheless they are threatening seriously patient's life because a critical illness as gastrointestinal hemorrhage in such casses hit patients whose vital functions are compromised from recent thoracic surgery. The pathogenetic questions, as well critical problems of diagnosis and treatment, are discussed on the basis of three observed cases.", "contents": "[Postoperative gastrointestinal hemorrhages after pulmonary resections (author's transly]. Postoperative gastrointestinal hemorrhages are a infrequent complication of pulmonary resections. Nevertheless they are threatening seriously patient's life because a critical illness as gastrointestinal hemorrhage in such casses hit patients whose vital functions are compromised from recent thoracic surgery. The pathogenetic questions, as well critical problems of diagnosis and treatment, are discussed on the basis of three observed cases."}
{"id": "PMID:1088085", "title": "[Effect of damage to the zone of the medial forebrain bundle and preoptic area on the activiy of a penicillin-induced epileptiform focus (phenomenon of the hyperactive determinant dispatch station)].", "content": "It was shown in experiments on cats under nembutal anesthesia that a lesion of the medial forebrain bundle (MFB) and partly of the preoptic region at the side of local penicillin application on the cerebral cortex (g. suprasylvius medius) results in depression of the epileptiform activity in the penicillin-induced focus, as well as in the secondary \"mirror\" focus, which appeared in the symmetrical cortex area of the other hemisphere. The MFB lesion at the \"mirror\" focus side led to depression of the seizure spike potentials in this focus only and did not change the activity in the primary epileptiform one. The described effects are considered from the aspect of the conception on the role of the determinant dispatch station (DDS) in the central nervous system: the primary epileptiform focus plays the role of the hyperactive DDS which induces the secondary focus and determines the character of its activity. The results of the study substantiate a suggestion that the MFB can take part in the modulation of the cortical epileptiform activity.", "contents": "[Effect of damage to the zone of the medial forebrain bundle and preoptic area on the activiy of a penicillin-induced epileptiform focus (phenomenon of the hyperactive determinant dispatch station)]. It was shown in experiments on cats under nembutal anesthesia that a lesion of the medial forebrain bundle (MFB) and partly of the preoptic region at the side of local penicillin application on the cerebral cortex (g. suprasylvius medius) results in depression of the epileptiform activity in the penicillin-induced focus, as well as in the secondary \"mirror\" focus, which appeared in the symmetrical cortex area of the other hemisphere. The MFB lesion at the \"mirror\" focus side led to depression of the seizure spike potentials in this focus only and did not change the activity in the primary epileptiform one. The described effects are considered from the aspect of the conception on the role of the determinant dispatch station (DDS) in the central nervous system: the primary epileptiform focus plays the role of the hyperactive DDS which induces the secondary focus and determines the character of its activity. The results of the study substantiate a suggestion that the MFB can take part in the modulation of the cortical epileptiform activity."}
{"id": "PMID:1088086", "title": "[NAG-infection in grass frogs (Rana temporaria) subjected to hypothermia].", "content": "Rana temporaria kept under hypothermic conditions approaching anabiosis were inoculated with NAG-vibrios and examined clinically, bacteriologically, histologically, and electron microscopically. Oral inoculation of hypothermic frogs with NAG-vibrios resulted in 18 to 24 hours in the development of acute NAG-infection resembling the cholera-like syndrome, and characterized by general intoxication and local enteropathogenic effects. NAG-vibrios persisted in the frog gastrointestinal tract for a long time after the cessation of the acute period of the disease.", "contents": "[NAG-infection in grass frogs (Rana temporaria) subjected to hypothermia]. Rana temporaria kept under hypothermic conditions approaching anabiosis were inoculated with NAG-vibrios and examined clinically, bacteriologically, histologically, and electron microscopically. Oral inoculation of hypothermic frogs with NAG-vibrios resulted in 18 to 24 hours in the development of acute NAG-infection resembling the cholera-like syndrome, and characterized by general intoxication and local enteropathogenic effects. NAG-vibrios persisted in the frog gastrointestinal tract for a long time after the cessation of the acute period of the disease."}
{"id": "PMID:1088081", "title": "[Mathematical model of the immune response].", "content": "A model of immune reaction is suggested, ahich takes into account the delay in the development of an immune response. The model depending on parameters values describes: an asymptotic decrease of antigene quantity, approach of its quantity towards constant value, periodic course of the illness, unlimited growth of the antigene quantity. It is shown that the course of the reaction essentially depends on the duration of delay. Parameters regions corresponding to different regimes are determined.", "contents": "[Mathematical model of the immune response]. A model of immune reaction is suggested, ahich takes into account the delay in the development of an immune response. The model depending on parameters values describes: an asymptotic decrease of antigene quantity, approach of its quantity towards constant value, periodic course of the illness, unlimited growth of the antigene quantity. It is shown that the course of the reaction essentially depends on the duration of delay. Parameters regions corresponding to different regimes are determined."}
{"id": "PMID:1088087", "title": "[Phenomenon of human peripheral blood lymphocyte interaction with autologous erythrocytes].", "content": "Phenomenon of the lymphocyte interaction with autologous erythrocytes was revealed in the patients with auto-immune diseases. Macroscopically this phenomenon appeared in the form of hemagglutination, and microscopically--of \"rosette\" formation. No lymphocyte interaction with autologous erythrocytes was found in the group of normal subjects and patients without autoimmune disorders.", "contents": "[Phenomenon of human peripheral blood lymphocyte interaction with autologous erythrocytes]. Phenomenon of the lymphocyte interaction with autologous erythrocytes was revealed in the patients with auto-immune diseases. Macroscopically this phenomenon appeared in the form of hemagglutination, and microscopically--of \"rosette\" formation. No lymphocyte interaction with autologous erythrocytes was found in the group of normal subjects and patients without autoimmune disorders."}
{"id": "PMID:1088088", "title": "[Functional activity of splenic T- and B-cells during the process of tumor growth].", "content": "Functional activity of B- and T-cells was studied in mice during the growth of the inoculated tumours; it appeared that in the course of the tumour growth the capacity of B- and T-cells to the interaction in the immunization with sheep erythrocytes was disturbed. B-cells were affected earlier than T-cells.", "contents": "[Functional activity of splenic T- and B-cells during the process of tumor growth]. Functional activity of B- and T-cells was studied in mice during the growth of the inoculated tumours; it appeared that in the course of the tumour growth the capacity of B- and T-cells to the interaction in the immunization with sheep erythrocytes was disturbed. B-cells were affected earlier than T-cells."}
{"id": "PMID:1088082", "title": "[Histochemical studies of the sensitivity of frog retinal cells to stimulus movement and flashes].", "content": "The technique of quantitative UV-cytophotometry of RNA was used for comparative study of the sensitivity of frog retina cells to the movement of stimuli and flashings. A corresponding set of stimulators which includes the movement of mounted stimuli and heterogenous flashings of diffuse light showed that a type of cells exists in the interior nuclear layer of the retina which is specifically sensitive to the movement. It is suggested that in the formation of this property a dynamic reciprocal lateral inhibition on the level between bipolar and amacrine cells takes place. Application of heterogenous flashing light allowed also to reveal functional variety of bipolar cells.", "contents": "[Histochemical studies of the sensitivity of frog retinal cells to stimulus movement and flashes]. The technique of quantitative UV-cytophotometry of RNA was used for comparative study of the sensitivity of frog retina cells to the movement of stimuli and flashings. A corresponding set of stimulators which includes the movement of mounted stimuli and heterogenous flashings of diffuse light showed that a type of cells exists in the interior nuclear layer of the retina which is specifically sensitive to the movement. It is suggested that in the formation of this property a dynamic reciprocal lateral inhibition on the level between bipolar and amacrine cells takes place. Application of heterogenous flashing light allowed also to reveal functional variety of bipolar cells."}
{"id": "PMID:1088095", "title": "Progress in standardization: 4 immunological reagents.", "content": "The need for material standards in the field of clinical immunology, together with the mode of operation of the combined World Health Organization/International Union of Immunological Societies programme for the provision of such standards, are discussed. Attention is drawn to the importance of the use of International Units in reporting concentrations of complex constituents, e.g., immunoglobulins in body fluids, and to the availability of standard materials against which such components can be calibrated. The necessity for the standardization of nomenclature is also emphasized.", "contents": "Progress in standardization: 4 immunological reagents. The need for material standards in the field of clinical immunology, together with the mode of operation of the combined World Health Organization/International Union of Immunological Societies programme for the provision of such standards, are discussed. Attention is drawn to the importance of the use of International Units in reporting concentrations of complex constituents, e.g., immunoglobulins in body fluids, and to the availability of standard materials against which such components can be calibrated. The necessity for the standardization of nomenclature is also emphasized."}
{"id": "PMID:1088089", "title": "Prolonged medial forebrain bundle unit responses to rewarding and aversive intracranial stimuli.", "content": "In unanesthetized cerveau isol\u00e9 rats, brief medial forebrain bundle (MFB) and dorsal midbrain reticular (RET) stimulus trains elicited prolonged MFB unit responses lasting up to ten or more seconds. On the MFB unit responses studied, the effects of MFB and RET stimuli were basically similar, although the stimulations were probably rewarding and aversive respectively. These data agree with the previously reported anatomical localization, in medial regions of thalamus and pallidum, of opposite single cell responses to the behaviorally opposite inputs, and suggest that unit responses to rewarding stimuli should not be characterized as reward-related when aversive stimuli elicit similar responses in the same unit.", "contents": "Prolonged medial forebrain bundle unit responses to rewarding and aversive intracranial stimuli. In unanesthetized cerveau isol\u00e9 rats, brief medial forebrain bundle (MFB) and dorsal midbrain reticular (RET) stimulus trains elicited prolonged MFB unit responses lasting up to ten or more seconds. On the MFB unit responses studied, the effects of MFB and RET stimuli were basically similar, although the stimulations were probably rewarding and aversive respectively. These data agree with the previously reported anatomical localization, in medial regions of thalamus and pallidum, of opposite single cell responses to the behaviorally opposite inputs, and suggest that unit responses to rewarding stimuli should not be characterized as reward-related when aversive stimuli elicit similar responses in the same unit."}
{"id": "PMID:1088090", "title": "Infant rats: hypothalamic unit activity.", "content": "Single-unit recordings were made from lateral hypothalamic area (LHA) neurons of infant (8 through 21 days of age) male and female albino rats. Basal (or spontaneous) firing rates of LHA neurons were recorded for 20 min from rats 8 through 21 days of age, and mean basal firing rates did not differ across ages. Basal firing rates of LHA neurons in infant rats ranged from less than 1 spike/sec up to 27 spikes/sec, with 56% of the basal rates less than 1 spike/sec. Infant rats also received hypertonic saline injections (and control injections) to determine osmosensitivity characteristics of LHA neurons. Across ages, 56%--88% of LHA neurons were osmosensitive, with the highest percentages of osmosensitive units found in rats 14--21 days of age. Infant rats showed LHA unit electrophysiological characteristics that were quite similar to those obtained from LHA neurons in adult rats with respect to basal firing rates and osmosensitivity, however, LHA neuron activity in infants was not modulated by sensory stimuli as is frequently reported for adults. The results are discussed in terms of functional development of the LHA.", "contents": "Infant rats: hypothalamic unit activity. Single-unit recordings were made from lateral hypothalamic area (LHA) neurons of infant (8 through 21 days of age) male and female albino rats. Basal (or spontaneous) firing rates of LHA neurons were recorded for 20 min from rats 8 through 21 days of age, and mean basal firing rates did not differ across ages. Basal firing rates of LHA neurons in infant rats ranged from less than 1 spike/sec up to 27 spikes/sec, with 56% of the basal rates less than 1 spike/sec. Infant rats also received hypertonic saline injections (and control injections) to determine osmosensitivity characteristics of LHA neurons. Across ages, 56%--88% of LHA neurons were osmosensitive, with the highest percentages of osmosensitive units found in rats 14--21 days of age. Infant rats showed LHA unit electrophysiological characteristics that were quite similar to those obtained from LHA neurons in adult rats with respect to basal firing rates and osmosensitivity, however, LHA neuron activity in infants was not modulated by sensory stimuli as is frequently reported for adults. The results are discussed in terms of functional development of the LHA."}
{"id": "PMID:1088096", "title": "Observations on the swarming and mating behavior of Anopheles culicifacies Giles in nature.", "content": "The mating and swarming behaviour of A. culicifacies Giles was investigated during December 1975 at a cattle shed near the village of Sattoki, Lahore District, Punjab, Pakistan. On average, swarming commenced 20.9 min before sunset (light intensity 1414.4 lx) and ended 21.0 min after sunset (5.4 lx) with pairing restricted to the period from 6.1 min before (467.2 lx) to 15.8 min after sunset (26.9 lx). The swarms were principally composed of males, with females entering only for mating. On average, copulation lasted 27.2 s and was completed in flight. Most females (71.8%) collected while mating had taken a partial blood meal either the previous evening or on the same evening as mating. All females in the swarms were nulliparous and 82.6% had ovaries developed to at least Christophers' stage IIa.", "contents": "Observations on the swarming and mating behavior of Anopheles culicifacies Giles in nature. The mating and swarming behaviour of A. culicifacies Giles was investigated during December 1975 at a cattle shed near the village of Sattoki, Lahore District, Punjab, Pakistan. On average, swarming commenced 20.9 min before sunset (light intensity 1414.4 lx) and ended 21.0 min after sunset (5.4 lx) with pairing restricted to the period from 6.1 min before (467.2 lx) to 15.8 min after sunset (26.9 lx). The swarms were principally composed of males, with females entering only for mating. On average, copulation lasted 27.2 s and was completed in flight. Most females (71.8%) collected while mating had taken a partial blood meal either the previous evening or on the same evening as mating. All females in the swarms were nulliparous and 82.6% had ovaries developed to at least Christophers' stage IIa."}
{"id": "PMID:1088097", "title": "Field studies of a rapid, accurate means of quantifying Schistosoma haematobium eggs in urine samples.", "content": "A new means of quantifying schistosome eggs has been adapted from the laboratory to the field. Urine from 510 individuals in areas of Kenya with a high prevalence of schistosomiasis haematobia was injected in aliquots of 10, 5 and 1 ml on to transparent Nuclepore filters, 13 mm in diameter. The filters were placed face down on glass slides and were read without staining at x40 magnification. The method has been shown to be accurate, sensitive, and reproducible, and also extremely rapid.", "contents": "Field studies of a rapid, accurate means of quantifying Schistosoma haematobium eggs in urine samples. A new means of quantifying schistosome eggs has been adapted from the laboratory to the field. Urine from 510 individuals in areas of Kenya with a high prevalence of schistosomiasis haematobia was injected in aliquots of 10, 5 and 1 ml on to transparent Nuclepore filters, 13 mm in diameter. The filters were placed face down on glass slides and were read without staining at x40 magnification. The method has been shown to be accurate, sensitive, and reproducible, and also extremely rapid."}
{"id": "PMID:1088098", "title": "Effect of sulfadoxine on transmission of Vibrio cholerae infection among family contacts of cholera patients in Calcutta.", "content": "Sulfadoxine, a long-acting sulfonamide, and tetracycline were compared as regards their effectiveness in reducing transmission of cholera infection among the contacts of cholera patients in Calcutta. A total of 109 healthy family contacts of confirmed hospitalized cholera patients were treated with a single oral dose of sulfadoxine graded according to age. Another similar group of 101 contacts received 6 divided doses of oral tetracycline over a period of 3 days. All these contacts were bacteriologically examined for 15 days. Results showed that tetracycline was effective in significantly reducing the load of cholera infection from the 2nd to 6th day, while sulfadoxine was effective from the 3rd to the 6th day. The advantages and disadvantages of the two drugs as chemoprophylactic agents in cholera are discussed.", "contents": "Effect of sulfadoxine on transmission of Vibrio cholerae infection among family contacts of cholera patients in Calcutta. Sulfadoxine, a long-acting sulfonamide, and tetracycline were compared as regards their effectiveness in reducing transmission of cholera infection among the contacts of cholera patients in Calcutta. A total of 109 healthy family contacts of confirmed hospitalized cholera patients were treated with a single oral dose of sulfadoxine graded according to age. Another similar group of 101 contacts received 6 divided doses of oral tetracycline over a period of 3 days. All these contacts were bacteriologically examined for 15 days. Results showed that tetracycline was effective in significantly reducing the load of cholera infection from the 2nd to 6th day, while sulfadoxine was effective from the 3rd to the 6th day. The advantages and disadvantages of the two drugs as chemoprophylactic agents in cholera are discussed."}
{"id": "PMID:1088099", "title": "Doxycycline in the treatment of cholera.", "content": "Doxycycline was compared with tetracycline in the treatment of cholera. Four types of treatment were compared: Group A was given 200 mg of doxycycline on admission and 100 mg on the second day; Group B was given 200 mg of doxycycline on admission only; Group C was given 300 mg of doxycycline on admission only; and Group D received 500 mg of tetracycline every 6 h for 48 h. Tetracycline showed a slight advantage in respect of duration of diarrhoea and vibrio excretion compared with doxycycline given as a single dose of 300 mg, but fluid intake and output were about the same in these two groups. The other two doxycycline treatment schedules did not compare well with tetracycline treatment.", "contents": "Doxycycline in the treatment of cholera. Doxycycline was compared with tetracycline in the treatment of cholera. Four types of treatment were compared: Group A was given 200 mg of doxycycline on admission and 100 mg on the second day; Group B was given 200 mg of doxycycline on admission only; Group C was given 300 mg of doxycycline on admission only; and Group D received 500 mg of tetracycline every 6 h for 48 h. Tetracycline showed a slight advantage in respect of duration of diarrhoea and vibrio excretion compared with doxycycline given as a single dose of 300 mg, but fluid intake and output were about the same in these two groups. The other two doxycycline treatment schedules did not compare well with tetracycline treatment."}
{"id": "PMID:1088100", "title": "Global surveillance of antibiotic sensitivity of Vibrio cholerae.", "content": "Strains of Vibrio cholerae-1156 from various parts of the world-were examined by standardized antibiotic sensitivity tests in one centre, to determine the global incidence of antibiotic resistance in this organism and to assess the extent to which differences in methods of sensitivity testing might be responsible for discrepancies in the reported incidence of resistant strains. Of the strains examined, 1127 were fully sensitive to ampicillin, chloramphenicol, tetracycline, furazolidone, and three different sulphonamides, 27 showed stable and reproducible resistance to one or more of these agents, and 2 proved to contain a minority of cells with unstable, presumably plasmid-borne, resistance to chloram-phenicol. Unstable resistance to antibiotics may be common in V. cholerae but rarely recognized, and may account for some of the discrepancies in the reported incidence of resistant strains.", "contents": "Global surveillance of antibiotic sensitivity of Vibrio cholerae. Strains of Vibrio cholerae-1156 from various parts of the world-were examined by standardized antibiotic sensitivity tests in one centre, to determine the global incidence of antibiotic resistance in this organism and to assess the extent to which differences in methods of sensitivity testing might be responsible for discrepancies in the reported incidence of resistant strains. Of the strains examined, 1127 were fully sensitive to ampicillin, chloramphenicol, tetracycline, furazolidone, and three different sulphonamides, 27 showed stable and reproducible resistance to one or more of these agents, and 2 proved to contain a minority of cells with unstable, presumably plasmid-borne, resistance to chloram-phenicol. Unstable resistance to antibiotics may be common in V. cholerae but rarely recognized, and may account for some of the discrepancies in the reported incidence of resistant strains."}
{"id": "PMID:1088101", "title": "Value of phage typing of Vibrio cholerae biotype eltor in West Africa.", "content": "The epidemiological value of phage typing of El Tor vibrios in West Africa was assessed by testing 211 representative strains from all outbreaks of the cholera epidemic in Togo (1970-73) with the prophage typing method of Nicolle et al. and the lytic El Tor phages of Basu & Mukerjee. Prophage typing proved to be of limited epidemiological value in Togo since 203 of the 211 strains tested belonged to the same phage type-namely, type 2 of Nicolle et al., which corresponds to the Celebes type of Takeya. Six strains belonged to type 1, 1 strain to type 4, and 1 strain was untypable.Tested by the lytic El Tor phages, 175 strains were found to belong to type 4 of Basu & Mukerjee, 35 strains to type 1, and 1 strain was untypable. All but two strains of type 1 were isolated from 3 outbreaks in which this phage type was found almost exclusively. The results are in good agreement with epidemiological data collected during the epidemic. El Tor phage V reacted with a specificity of over 99% when tested against 601 strains of El Tor and NCV vibrios from Togo and may be recommended for the rapid identification of El Tor vibrios.", "contents": "Value of phage typing of Vibrio cholerae biotype eltor in West Africa. The epidemiological value of phage typing of El Tor vibrios in West Africa was assessed by testing 211 representative strains from all outbreaks of the cholera epidemic in Togo (1970-73) with the prophage typing method of Nicolle et al. and the lytic El Tor phages of Basu & Mukerjee. Prophage typing proved to be of limited epidemiological value in Togo since 203 of the 211 strains tested belonged to the same phage type-namely, type 2 of Nicolle et al., which corresponds to the Celebes type of Takeya. Six strains belonged to type 1, 1 strain to type 4, and 1 strain was untypable.Tested by the lytic El Tor phages, 175 strains were found to belong to type 4 of Basu & Mukerjee, 35 strains to type 1, and 1 strain was untypable. All but two strains of type 1 were isolated from 3 outbreaks in which this phage type was found almost exclusively. The results are in good agreement with epidemiological data collected during the epidemic. El Tor phage V reacted with a specificity of over 99% when tested against 601 strains of El Tor and NCV vibrios from Togo and may be recommended for the rapid identification of El Tor vibrios."}
{"id": "PMID:1088102", "title": "Type B and non-B viral hepatitis in Jerusalem.", "content": "In a two-year survey of adult patients hospitalized with acute viral hepatitis in Jerusalem, 27% were reactive for the hepatitis B surface antigen (HB(s)Ag) by radioimmunoassay and therefore diagnosed as having hepatitis B. The majority of patients (73%) were non-reactive for HB(s)Ag and their diagnosis was non-B hepatitis (\"type unspecifiable\"). Thirty-one per cent of patients with hepatitis B and only 5% of patients with non-B hepatitis had histories consistent with parenteral transmission of hepatitis by blood transfusion or drug use. An additional 19% of the patients with hepatitis B had possible parenteral exposure and 50% had no obvious parenteral exposure, indicating that non-parenteral transmission of the hepatitis B virus (HBV) may be a significant epidemiological factor (50-69%) in the Jerusalem area. The prominent role of non-parenteral transmission of HBV is further evidenced by the relatively high prevalences of HB(s)Ag (0.97%) and antibody to hepatitis B surface antigen (anti-HB(s)) (19.0%) in healthy blood donors. These findings are consistent with the view that personal contact and intra-familial spread may be important factors in the epidemiology of HBV and indicate that non-parenterally transmitted HBV contributes significantly to endemic viral hepatitis in the Jerusalem area.", "contents": "Type B and non-B viral hepatitis in Jerusalem. In a two-year survey of adult patients hospitalized with acute viral hepatitis in Jerusalem, 27% were reactive for the hepatitis B surface antigen (HB(s)Ag) by radioimmunoassay and therefore diagnosed as having hepatitis B. The majority of patients (73%) were non-reactive for HB(s)Ag and their diagnosis was non-B hepatitis (\"type unspecifiable\"). Thirty-one per cent of patients with hepatitis B and only 5% of patients with non-B hepatitis had histories consistent with parenteral transmission of hepatitis by blood transfusion or drug use. An additional 19% of the patients with hepatitis B had possible parenteral exposure and 50% had no obvious parenteral exposure, indicating that non-parenteral transmission of the hepatitis B virus (HBV) may be a significant epidemiological factor (50-69%) in the Jerusalem area. The prominent role of non-parenteral transmission of HBV is further evidenced by the relatively high prevalences of HB(s)Ag (0.97%) and antibody to hepatitis B surface antigen (anti-HB(s)) (19.0%) in healthy blood donors. These findings are consistent with the view that personal contact and intra-familial spread may be important factors in the epidemiology of HBV and indicate that non-parenterally transmitted HBV contributes significantly to endemic viral hepatitis in the Jerusalem area."}
{"id": "PMID:1088103", "title": "Rough determination of the cost-benefit balance point of sanitation programmes.", "content": "Resources for sanitation programmes in developing countries are limited and therefore must be used judiciously to obtain the best possible effect. Cost-benefit analysis is a tool that permits the better utilization of available resources. A simple method for rough determination of the cost-benefit balance point has been devised which requires little computation. To reduce the computations to a minimum, nomograms have been constructed which require little or no mathematical skill for their use. While the method falls short of perfection, its simplicity makes it useful for a rough evaluation of the benefits from sanitation programmes aimed at disease control in countries whose resources are not available for more sophisticated analysis.", "contents": "Rough determination of the cost-benefit balance point of sanitation programmes. Resources for sanitation programmes in developing countries are limited and therefore must be used judiciously to obtain the best possible effect. Cost-benefit analysis is a tool that permits the better utilization of available resources. A simple method for rough determination of the cost-benefit balance point has been devised which requires little computation. To reduce the computations to a minimum, nomograms have been constructed which require little or no mathematical skill for their use. While the method falls short of perfection, its simplicity makes it useful for a rough evaluation of the benefits from sanitation programmes aimed at disease control in countries whose resources are not available for more sophisticated analysis."}
{"id": "PMID:1088104", "title": "Exposure to pesticides in agriculture: a survey of spraymen using dimethoate in the Sudan.", "content": "There is a need for objective data on the exposure of spraymen to pesticides in agriculture in order to assess minimum requirements for practicable protection in tropical areas. To provide data, a survey was carried out in the Sudan on spraymen using dimethoate. No cholinesterase depressions were found and the calculated dose received by each man per day was within safe limits in the circumstances of this survey. Respiratory exposure was only a minor part of total exposure. The methodology of such surveys is discussed and the need for the use of a standard protocol is emphasized.", "contents": "Exposure to pesticides in agriculture: a survey of spraymen using dimethoate in the Sudan. There is a need for objective data on the exposure of spraymen to pesticides in agriculture in order to assess minimum requirements for practicable protection in tropical areas. To provide data, a survey was carried out in the Sudan on spraymen using dimethoate. No cholinesterase depressions were found and the calculated dose received by each man per day was within safe limits in the circumstances of this survey. Respiratory exposure was only a minor part of total exposure. The methodology of such surveys is discussed and the need for the use of a standard protocol is emphasized."}
{"id": "PMID:1088105", "title": "Validating a method of psychiatric case identification in Jamaica.", "content": "There is an urgent need for simple, reliable instruments for psychiatric research in developing countries. Limited resources, however, make the development of instruments de novo difficult. A simple method for establishing the validity of a method of case identification in a new population is described. A shortened version of a standard general health questionnaire was shown to be an effective method of identifying non-psychotic disorders in Jamaica, subject to a recalibration.", "contents": "Validating a method of psychiatric case identification in Jamaica. There is an urgent need for simple, reliable instruments for psychiatric research in developing countries. Limited resources, however, make the development of instruments de novo difficult. A simple method for establishing the validity of a method of case identification in a new population is described. A shortened version of a standard general health questionnaire was shown to be an effective method of identifying non-psychotic disorders in Jamaica, subject to a recalibration."}
{"id": "PMID:1088106", "title": "A comparison of serological tests for detecting antibody to plague.", "content": "A study was undertaken of the relationship between the passive haemagglutination and haemagglutination inhibition tests used in serum surveys for specific plague antibodies. Both techniques gave similar titres for sera of albino Rattus norvegicus convalescent from plague infection. They appear to be equally suitable for plague surveillance and control programmes.", "contents": "A comparison of serological tests for detecting antibody to plague. A study was undertaken of the relationship between the passive haemagglutination and haemagglutination inhibition tests used in serum surveys for specific plague antibodies. Both techniques gave similar titres for sera of albino Rattus norvegicus convalescent from plague infection. They appear to be equally suitable for plague surveillance and control programmes."}
{"id": "PMID:1088107", "title": "Chlamydiae as agents of sexually transmitted diseases.", "content": "Chlamydiae are being increasingly recognized as an important cause of human disease. The known geographical distribution of lymphogranuloma venereum and the role of chlamydiae as agents of sexually transmitted diseases are reviewed. The presence of chlamydiae in the urethra and the cervix, and their etiological relationship to genital infections, first recognized in connexion with ocular infections, have been proved in a number of studies in selected populations in a few countries. Chlamydiae appear to be the most important agent of nongonococcal urethritis, which in some cases appears now to be more frequent than gonococcal urethritis. In addition to their association with cervicitis, chlamydiae appear also to be fairly frequent in the cervix of apparently normal, asymptomatic, and sexually active women. The role of chlamydiae as agents of other human diseases still requires to be clarified. The organisms have been found in association with pelvic inflammatory disease, neonatal pneumonia, pharyngitis, and otitis. There is need for additional studies in view of the fact that effective chemotherapy is available. An outline is given of laboratory methods that may be useful for the diagnosis of chlamydial infections.", "contents": "Chlamydiae as agents of sexually transmitted diseases. Chlamydiae are being increasingly recognized as an important cause of human disease. The known geographical distribution of lymphogranuloma venereum and the role of chlamydiae as agents of sexually transmitted diseases are reviewed. The presence of chlamydiae in the urethra and the cervix, and their etiological relationship to genital infections, first recognized in connexion with ocular infections, have been proved in a number of studies in selected populations in a few countries. Chlamydiae appear to be the most important agent of nongonococcal urethritis, which in some cases appears now to be more frequent than gonococcal urethritis. In addition to their association with cervicitis, chlamydiae appear also to be fairly frequent in the cervix of apparently normal, asymptomatic, and sexually active women. The role of chlamydiae as agents of other human diseases still requires to be clarified. The organisms have been found in association with pelvic inflammatory disease, neonatal pneumonia, pharyngitis, and otitis. There is need for additional studies in view of the fact that effective chemotherapy is available. An outline is given of laboratory methods that may be useful for the diagnosis of chlamydial infections."}
{"id": "PMID:1088108", "title": "Large-scale use of freeze-dried smallpox vaccine prepared in primary cultures of rabbit kidney cells.", "content": "A lyophilized smallpox vaccine made from infected monolayer cultures of primary rabbit kidney cells was used together with a calf lymph vaccine in a field trial in Lombok, Indonesia, in 1973. About 60 000 children below 15 years of age were vaccinated: some 50 000 with the tissue culture vaccine and about 10 000 with calf lymph vaccine. Similar results were obtained with both vaccines in primary vaccinees and in revaccinees as regards the take rate, pock reactions, and serious secondary reactions.", "contents": "Large-scale use of freeze-dried smallpox vaccine prepared in primary cultures of rabbit kidney cells. A lyophilized smallpox vaccine made from infected monolayer cultures of primary rabbit kidney cells was used together with a calf lymph vaccine in a field trial in Lombok, Indonesia, in 1973. About 60 000 children below 15 years of age were vaccinated: some 50 000 with the tissue culture vaccine and about 10 000 with calf lymph vaccine. Similar results were obtained with both vaccines in primary vaccinees and in revaccinees as regards the take rate, pock reactions, and serious secondary reactions."}
{"id": "PMID:1088109", "title": "Preliminary parasitological results of a pilot mollusciciding campaign to control transmission of Schistosoma mansoni in St Lucia.", "content": "A mollusciciding campaign was begun in Cul-de-Sac Valley, St Lucia, at the end of 1970, following several years of epidemiological studies in which transmission of Schistosoma mansoni was found to be high in settlements on the valley floor but low in hillside settlements. Postcontrol (1971-73) findings in children, when compared with precontrol data and with data from an adjacent valley having a similar transmission pattern, show significant reductions in prevalence, incidence, and intensity of infection.", "contents": "Preliminary parasitological results of a pilot mollusciciding campaign to control transmission of Schistosoma mansoni in St Lucia. A mollusciciding campaign was begun in Cul-de-Sac Valley, St Lucia, at the end of 1970, following several years of epidemiological studies in which transmission of Schistosoma mansoni was found to be high in settlements on the valley floor but low in hillside settlements. Postcontrol (1971-73) findings in children, when compared with precontrol data and with data from an adjacent valley having a similar transmission pattern, show significant reductions in prevalence, incidence, and intensity of infection."}
{"id": "PMID:1088110", "title": "Medical management of mycetoma.", "content": "A number of drugs and drug combinations were tested both in vitro and in vivo against pathogens causing actinomycetoma and maduromycetoma. Of 144 patients with actinomycetoma treated, 63.2% were cured, 21.5% showed great improvement in their condition, and 11.1% showed some improvement. Treatment was successful even when there was advanced bone involvement. The most effective treatments involved dapsone plus streptomycin sulfate or sulfamethoxazole-trimethoprim plus streptomycin. Sulfadoxine-pyrimethamine plus streptomycin or rifampicin plus streptomycin also gave good results. Griseofulvin plus penicillin showed some promise in the management of maduromycetoma caused by Madurella mycetomi but at the present time this should be used only as an adjunct to surgery. All mycetoma patients undergoing drug therapy should be carefully monitored and treatment should be temporarily suspended if anaemia or leucopenia develop.", "contents": "Medical management of mycetoma. A number of drugs and drug combinations were tested both in vitro and in vivo against pathogens causing actinomycetoma and maduromycetoma. Of 144 patients with actinomycetoma treated, 63.2% were cured, 21.5% showed great improvement in their condition, and 11.1% showed some improvement. Treatment was successful even when there was advanced bone involvement. The most effective treatments involved dapsone plus streptomycin sulfate or sulfamethoxazole-trimethoprim plus streptomycin. Sulfadoxine-pyrimethamine plus streptomycin or rifampicin plus streptomycin also gave good results. Griseofulvin plus penicillin showed some promise in the management of maduromycetoma caused by Madurella mycetomi but at the present time this should be used only as an adjunct to surgery. All mycetoma patients undergoing drug therapy should be carefully monitored and treatment should be temporarily suspended if anaemia or leucopenia develop."}
{"id": "PMID:1088111", "title": "Nutritional status of children in Nepal, 1975.", "content": "Using a random start, fixed interval method of population proportionate sampling, the survey teams visited 219 villages in rural Nepal and collected data on height, weight, and age from 6501 preschool children. During training and at specific times during the survey, systematic standardization methods were utilized to improve the quality of the collected data. Using 80% of the reference median weight-for-height as the cut-off point, the prevalence of acute undernutrition was 6.7%. Using 90% of the reference median height-for-age as the cut-off point, the prevalence of chronic undernutrition was 52%. The prevalence of chronic undernutrition was significantly higher in the hilly areas. Mean haemoglobin values of blood samples collected from one-fifth of the survey population increased with age with no difference between hill and terai areas. A Nepal urban elite population was measured for comparison. The survey method used indicators that are economical in terms of money, time, and personnel and that provide objective data on the extent and distribution of protein/energy undernutrition and of low haemoglobin values in preschool children.", "contents": "Nutritional status of children in Nepal, 1975. Using a random start, fixed interval method of population proportionate sampling, the survey teams visited 219 villages in rural Nepal and collected data on height, weight, and age from 6501 preschool children. During training and at specific times during the survey, systematic standardization methods were utilized to improve the quality of the collected data. Using 80% of the reference median weight-for-height as the cut-off point, the prevalence of acute undernutrition was 6.7%. Using 90% of the reference median height-for-age as the cut-off point, the prevalence of chronic undernutrition was 52%. The prevalence of chronic undernutrition was significantly higher in the hilly areas. Mean haemoglobin values of blood samples collected from one-fifth of the survey population increased with age with no difference between hill and terai areas. A Nepal urban elite population was measured for comparison. The survey method used indicators that are economical in terms of money, time, and personnel and that provide objective data on the extent and distribution of protein/energy undernutrition and of low haemoglobin values in preschool children."}
{"id": "PMID:1088113", "title": "Residues of DDT and its metabolites in human blood samples in Delhi, India.", "content": "Blood samples from 182 people in Delhi, India, were examined for DDT residues. All except 8 contained DDT and its metabolites. The average total DDT concentration in the whole blood ranged from 0.177 to 0.683 mg/litre in males and from 0.166 to 0.329 mg/litre in females. The DDT metabolites detected were p,p'-DDE, p,p'-DDD, and o,p'-DDT. DDE accounted for most of the total DDT.", "contents": "Residues of DDT and its metabolites in human blood samples in Delhi, India. Blood samples from 182 people in Delhi, India, were examined for DDT residues. All except 8 contained DDT and its metabolites. The average total DDT concentration in the whole blood ranged from 0.177 to 0.683 mg/litre in males and from 0.166 to 0.329 mg/litre in females. The DDT metabolites detected were p,p'-DDE, p,p'-DDD, and o,p'-DDT. DDE accounted for most of the total DDT."}
{"id": "PMID:1088114", "title": "Resistance of Salmonella serotypes to chloramphenicol.", "content": "A total of 704 Salmonella strains isolated from various sources were tested for their resistance to chloramphenicol. The results indicate that 36 out of 46 serotypes, comprising 671 strains (95.4%), were sensitive to chloramphenicol up to a level of 10 mug per ml of medium. Most of the strains were sensitive at concentrations between 2 mug and 4 mug of chloramphenicol per ml of medium. None of the strains isolated from animals, including poultry, was resistant. The results indicate that in India resistance of Salmonella strains to chloramphenicol has not increased over the years though this may have occurred in other parts of the world.", "contents": "Resistance of Salmonella serotypes to chloramphenicol. A total of 704 Salmonella strains isolated from various sources were tested for their resistance to chloramphenicol. The results indicate that 36 out of 46 serotypes, comprising 671 strains (95.4%), were sensitive to chloramphenicol up to a level of 10 mug per ml of medium. Most of the strains were sensitive at concentrations between 2 mug and 4 mug of chloramphenicol per ml of medium. None of the strains isolated from animals, including poultry, was resistant. The results indicate that in India resistance of Salmonella strains to chloramphenicol has not increased over the years though this may have occurred in other parts of the world."}
{"id": "PMID:1088116", "title": "Periaortic denervation in reconstructive surgery of coronary vessels.", "content": "Stimulation of sympathetic nervous conductive pathways and ganglia innervating the heart leads to a sudden increase in the cardiac performance and to a qualitative change in the phasic blood flow within the coronary vascular bed. This so-called malignant increase in the blood flow is accompanied by relative coronary insufficiency and increased mechanical stress imposed upon the coronary arteries. A denervation operation according to Arnulf does not prevent the propagation of excitation from the right stellate ganglion to the heart. A complete denervation is obtained by additional cutting of the right-side postganglional fibres. Then in a dog no changes occur in the coronary arterial blood flow measured at rest during the operation. In reconstructive surgery of coronary vessels in man, after denervation the peroperative blood flow in the aortocoronary bypass to the anterior interventicular branch increases. After a surgical denervation operation, the prevention of propagation of sudden neurogenic stimuli via the cervical sympathetic segment to the heart ventricles is the most striking result. Such a \"protection\" of the heart might retard the development rate of coronary insufficiency accompanying the ischaemic heart disease.", "contents": "Periaortic denervation in reconstructive surgery of coronary vessels. Stimulation of sympathetic nervous conductive pathways and ganglia innervating the heart leads to a sudden increase in the cardiac performance and to a qualitative change in the phasic blood flow within the coronary vascular bed. This so-called malignant increase in the blood flow is accompanied by relative coronary insufficiency and increased mechanical stress imposed upon the coronary arteries. A denervation operation according to Arnulf does not prevent the propagation of excitation from the right stellate ganglion to the heart. A complete denervation is obtained by additional cutting of the right-side postganglional fibres. Then in a dog no changes occur in the coronary arterial blood flow measured at rest during the operation. In reconstructive surgery of coronary vessels in man, after denervation the peroperative blood flow in the aortocoronary bypass to the anterior interventicular branch increases. After a surgical denervation operation, the prevention of propagation of sudden neurogenic stimuli via the cervical sympathetic segment to the heart ventricles is the most striking result. Such a \"protection\" of the heart might retard the development rate of coronary insufficiency accompanying the ischaemic heart disease."}
{"id": "PMID:1088117", "title": "Primary mediastinal choriocarcinoma in a man.", "content": "Choriocarcinoma with a primary site in the chest is rarely found. Characteristically, it is seen in young men presenting with cough, gynecomastia and chest pain. The disease is invariably fatal. The presently accepted therapy (triple therapy), consisting of methotrexate, actinomycin D and chlorambucil, has been unsuccessful to date.", "contents": "Primary mediastinal choriocarcinoma in a man. Choriocarcinoma with a primary site in the chest is rarely found. Characteristically, it is seen in young men presenting with cough, gynecomastia and chest pain. The disease is invariably fatal. The presently accepted therapy (triple therapy), consisting of methotrexate, actinomycin D and chlorambucil, has been unsuccessful to date."}
{"id": "PMID:1088118", "title": "Ischemic myocardial injury following aorto-coronary bypass surgery.", "content": "To assess the incidence of acute myocardial injury following aorto-coronary bypass surgery 151 patients (136 men and 15 women) were evaluated by studying serial preoperative and postoperative ECGs and SGOT, LDH and CPK levels. The mean age of men was 49.1 and of women, 53.1 years. Following surgery 15 patients (group I, 9.9 percent) developed new myocardial infarction as judged by Q wave criteria, 33 patients (group II, 22.5 percent) developed significant ST-T changes, and 103 patients (group III, 68 percent) had no significant ECG change. Mean postoperative SGOT values were: group I,126.2; group II, 100.6; and group III, 72.8. Only the difference in SGOT values between group I and III was significant (P less than 0.01). There was no correlation between type and site of surgery and the incidence of myocardial infarction. There were five deaths (3.3 percent). The combination of death and diagnosed myocardial infarction amounted to 12.6 percent of patients for this widely used elective procedure.", "contents": "Ischemic myocardial injury following aorto-coronary bypass surgery. To assess the incidence of acute myocardial injury following aorto-coronary bypass surgery 151 patients (136 men and 15 women) were evaluated by studying serial preoperative and postoperative ECGs and SGOT, LDH and CPK levels. The mean age of men was 49.1 and of women, 53.1 years. Following surgery 15 patients (group I, 9.9 percent) developed new myocardial infarction as judged by Q wave criteria, 33 patients (group II, 22.5 percent) developed significant ST-T changes, and 103 patients (group III, 68 percent) had no significant ECG change. Mean postoperative SGOT values were: group I,126.2; group II, 100.6; and group III, 72.8. Only the difference in SGOT values between group I and III was significant (P less than 0.01). There was no correlation between type and site of surgery and the incidence of myocardial infarction. There were five deaths (3.3 percent). The combination of death and diagnosed myocardial infarction amounted to 12.6 percent of patients for this widely used elective procedure."}
{"id": "PMID:1088127", "title": "Effect of hypothalamic surgery on prolactin release induced by 5-hydroxytryptophan (5-HTP) in rats.", "content": "Intravenous injections of varying doses of 5-HTP (1, 3 and 5 mg/100 g body wt), a precursor of serotonin, caused a significant and dose-related increase in plasma prolactin concentrations in urethane-anesthetized rats. Increases in plasma prolactin concentrations caused by 5-HTP (1 mg/100 g body wt iv) were abolished by the concomitant administration of L-DOPA (2 mg/100 g body wt iv). Plasma prolactin levels were also significantly elevated following the injection of 5-HTP in rats with complete hypothalamic deafferentation, whereas 5-HTP had no significant effect on plasma prolactin levels in rats with extensive hypothalamic ablation. These results suggest that 5-HTP causes prolactin secretion by stimulating the serotoninergic mechanism in the hypothalamus.", "contents": "Effect of hypothalamic surgery on prolactin release induced by 5-hydroxytryptophan (5-HTP) in rats. Intravenous injections of varying doses of 5-HTP (1, 3 and 5 mg/100 g body wt), a precursor of serotonin, caused a significant and dose-related increase in plasma prolactin concentrations in urethane-anesthetized rats. Increases in plasma prolactin concentrations caused by 5-HTP (1 mg/100 g body wt iv) were abolished by the concomitant administration of L-DOPA (2 mg/100 g body wt iv). Plasma prolactin levels were also significantly elevated following the injection of 5-HTP in rats with complete hypothalamic deafferentation, whereas 5-HTP had no significant effect on plasma prolactin levels in rats with extensive hypothalamic ablation. These results suggest that 5-HTP causes prolactin secretion by stimulating the serotoninergic mechanism in the hypothalamus."}
{"id": "PMID:1088129", "title": "Destruction of afferent nerve terminals in the inner ear of frog by aminooxyactic acid.", "content": "The drug aminooxyacetic acid, which inhibits GABA-transaminase, destroys the afferent nerve ending in the inner ear of the frog. The efferent nerve endings and the sensory cells are not affected.", "contents": "Destruction of afferent nerve terminals in the inner ear of frog by aminooxyactic acid. The drug aminooxyacetic acid, which inhibits GABA-transaminase, destroys the afferent nerve ending in the inner ear of the frog. The efferent nerve endings and the sensory cells are not affected."}
{"id": "PMID:1088135", "title": "Dissociation between cytotoxic activity and delayed hypersensitivity against syngeneic or allogeneic tumor grafts.", "content": "Cytotoxicity against tumor-specific antigen and allogeneic antigens in mice was raised by immunization with viable tumor cells in saline, but not by immunization with tumor cells in complete or incomplete Freund's adjuvant. On the other hand, strong delayed hypersensitivity as demonstrated by macrophage migration inhibition was detected in the mice immunized with tumor cells in complete or incomplete Freund's adjuvant in both syngeneic and allogeneic systems. After immunization with viable tumor cells, delayed hypersensitivity was raised to a moderate degree in allogeneic, but not at all in syngeneic mice. Cytotoxicity was retained after a booster with syngeneic tumor cells in complete Freund's adjuvant in the mice which had been immunized with viable tumor cells. Cytotoxicity was raised after a booster with viable syngeneic tumor cells in the mice which had been immunized with tumor cells in complete or incomplete Freund's adjuvant. Thus, the development of cytotoxicity depended entirely on the presence of viable tumor cells as an immunogen. Activation of helper T cells was not found in the syngeneic mice immunized with viable tumor cells or tumor cells in complete or incomplete Freund's adjuvant. In allogeneic mice, the activity was facilitated slightly by immunization with viable tumor cells or tumor cells in complete Fruend's adjuvant but not with tumor cells in incomplete Freund's adjuvant. This sytem may be useful for analytical studies on cellular immunity, since cytotoxicity, delayed hypersensitivity, and helper cell activation can be induced in dissociated forms.", "contents": "Dissociation between cytotoxic activity and delayed hypersensitivity against syngeneic or allogeneic tumor grafts. Cytotoxicity against tumor-specific antigen and allogeneic antigens in mice was raised by immunization with viable tumor cells in saline, but not by immunization with tumor cells in complete or incomplete Freund's adjuvant. On the other hand, strong delayed hypersensitivity as demonstrated by macrophage migration inhibition was detected in the mice immunized with tumor cells in complete or incomplete Freund's adjuvant in both syngeneic and allogeneic systems. After immunization with viable tumor cells, delayed hypersensitivity was raised to a moderate degree in allogeneic, but not at all in syngeneic mice. Cytotoxicity was retained after a booster with syngeneic tumor cells in complete Freund's adjuvant in the mice which had been immunized with viable tumor cells. Cytotoxicity was raised after a booster with viable syngeneic tumor cells in the mice which had been immunized with tumor cells in complete or incomplete Freund's adjuvant. Thus, the development of cytotoxicity depended entirely on the presence of viable tumor cells as an immunogen. Activation of helper T cells was not found in the syngeneic mice immunized with viable tumor cells or tumor cells in complete or incomplete Freund's adjuvant. In allogeneic mice, the activity was facilitated slightly by immunization with viable tumor cells or tumor cells in complete Fruend's adjuvant but not with tumor cells in incomplete Freund's adjuvant. This sytem may be useful for analytical studies on cellular immunity, since cytotoxicity, delayed hypersensitivity, and helper cell activation can be induced in dissociated forms."}
{"id": "PMID:1088136", "title": "[Position and bony structures of the vestibular apparatus in the guinea pig].", "content": "The study of the inner ear of the guinea pig intended to give an explanation to what extent there are differences discernible in relation to the human labyrinth. Additional histological research should clarify the question, if structural differences exist in the osseous labyrinth capsule of the same animal. It has turned out that in normal headbearing the position of the semicircular canals deviates from the human vestibular apparatus. The semicircular canals are nearly vertical to each other, but in comparison to the human labyrinth they are shifted around the longitudinal axis of the utriculus caudal by ca. 30 degrees. In general the position of the vestibulo-cochlear organ is fixed to a great extent by the inclined course of the petrosal pyramid. This different position of the semicircular canals in man and animal is supposed to be due to the phylogenetic evolution and the adjustment to upright walk. Size and extension of the single semicircular canals are very different within the same animal. These differences in size indicate causalities of form and function. The relations in the build of the osseous labyrinth are extremely complicated. Compared to the other corporal regions the static parts of the petrosal pyramid are exceptional massive and of remarkable hard consistency. In the inner capsule of the ear there are three different bone strata to be seen. The characteristic lamel structure is most solid nearest to the semicircular canals. The fetal characteristics in the maturing process of the petrosal bone were traced a long while in the postnatal life. The typical building of the labyrinthal bone structures contributes to the mechanical stability of the capsule.", "contents": "[Position and bony structures of the vestibular apparatus in the guinea pig]. The study of the inner ear of the guinea pig intended to give an explanation to what extent there are differences discernible in relation to the human labyrinth. Additional histological research should clarify the question, if structural differences exist in the osseous labyrinth capsule of the same animal. It has turned out that in normal headbearing the position of the semicircular canals deviates from the human vestibular apparatus. The semicircular canals are nearly vertical to each other, but in comparison to the human labyrinth they are shifted around the longitudinal axis of the utriculus caudal by ca. 30 degrees. In general the position of the vestibulo-cochlear organ is fixed to a great extent by the inclined course of the petrosal pyramid. This different position of the semicircular canals in man and animal is supposed to be due to the phylogenetic evolution and the adjustment to upright walk. Size and extension of the single semicircular canals are very different within the same animal. These differences in size indicate causalities of form and function. The relations in the build of the osseous labyrinth are extremely complicated. Compared to the other corporal regions the static parts of the petrosal pyramid are exceptional massive and of remarkable hard consistency. In the inner capsule of the ear there are three different bone strata to be seen. The characteristic lamel structure is most solid nearest to the semicircular canals. The fetal characteristics in the maturing process of the petrosal bone were traced a long while in the postnatal life. The typical building of the labyrinthal bone structures contributes to the mechanical stability of the capsule."}
{"id": "PMID:1088137", "title": "[Studies of gamma-oryzanol. (2) The antiulcerogenic action].", "content": "Investigations were performed in male Wistar rats to determine the effects of gamma-oryzanol on circadian rhythm of stress ulcer and on various kinds of experimental ulcers, and the modification of antiulcerogenic action of this drug when combined with the inhibitors or precursors of amine metabolism. Circadian rhythms were observed in stress ulceration with a peak at 2 : 00 p.m. and a reduction at 8 : 00 p.m. The inhibitory action of gamma-oryzanol was significant when stress was loaded for 6 hr from 2 : 00 a.m., 5 : 00 a.m., 11 : 00 a.m. or 2:00 p.m. An 8-day treatment with gamma-oryzanol, 100 mg/kg, s.c., showed a significant inhibition of fasting ulcer, while the 5-day pretreatment exerted slight effects on ulcers by pylorus-ligation or stress-atropine. A 10-day treatment with the same dose of gamma-oyzanol in acetic acid ulcers lowered the serum level of gastrin. Treatment with reserpine, alpha-MT or PCPA prior to stress loading abolished the anti-ulcer effect of gamma-oryzanol, 100 mg/kg, s.c. for 5 days in stress ulcer. Treatment with L-DOPA or 5-HTP revealed a tendency toward restoration of the anti-ulcer effect of gamma-oryzanol. It is suggested that the monoaminergic neuron system is involved in the anti-ulcer action of gamma-oryzanol.", "contents": "[Studies of gamma-oryzanol. (2) The antiulcerogenic action]. Investigations were performed in male Wistar rats to determine the effects of gamma-oryzanol on circadian rhythm of stress ulcer and on various kinds of experimental ulcers, and the modification of antiulcerogenic action of this drug when combined with the inhibitors or precursors of amine metabolism. Circadian rhythms were observed in stress ulceration with a peak at 2 : 00 p.m. and a reduction at 8 : 00 p.m. The inhibitory action of gamma-oryzanol was significant when stress was loaded for 6 hr from 2 : 00 a.m., 5 : 00 a.m., 11 : 00 a.m. or 2:00 p.m. An 8-day treatment with gamma-oryzanol, 100 mg/kg, s.c., showed a significant inhibition of fasting ulcer, while the 5-day pretreatment exerted slight effects on ulcers by pylorus-ligation or stress-atropine. A 10-day treatment with the same dose of gamma-oyzanol in acetic acid ulcers lowered the serum level of gastrin. Treatment with reserpine, alpha-MT or PCPA prior to stress loading abolished the anti-ulcer effect of gamma-oryzanol, 100 mg/kg, s.c. for 5 days in stress ulcer. Treatment with L-DOPA or 5-HTP revealed a tendency toward restoration of the anti-ulcer effect of gamma-oryzanol. It is suggested that the monoaminergic neuron system is involved in the anti-ulcer action of gamma-oryzanol."}
{"id": "PMID:1088138", "title": "Measurement of 5-hydroxyindole compounds during L-5-HTP treatment in depressed patients.", "content": "L-5-hydroxytryptophan (L-5-HTP), and immediate serotonin precursor, was given to the hospitalized depressed patients in an open clinical trial of the Phase 2 study for antidepressive effects of the agent. A relatively small dose, 150 mg orally for seven days, was employed, and seven of 14 patients responded to the treatment with mild or moderate amelioration of their depressive symptoms. Urinary excretion levels and plasma concentrations of three 5-hydroxyindole compounds, 5-HTP, 5-HT and 5-HIAA, were measured during the drug treatment. Approximately 70% of the orally administered dose of L-5-HTP was recovered from the urine of depressed patients. Major part of urinary indoleamine metabolites was free and conjugate 5-HIAA. Excretion levels of these compounds in urine were not consistently altered in the depressed patients as compared to those in normal subjects. Clinical response to L-5-HTP treatment  appeared to have some correlation with the biochemical measures in the depressed patients, that is, non-responders exhibited significantly lower excretion levels of 5-HT and 5-HIAA in urine, and lower plasma levels of 5-HT than responders. Administered L-5-HTP may not be fully utilized in the depressed patients who did not react to the agent.", "contents": "Measurement of 5-hydroxyindole compounds during L-5-HTP treatment in depressed patients. L-5-hydroxytryptophan (L-5-HTP), and immediate serotonin precursor, was given to the hospitalized depressed patients in an open clinical trial of the Phase 2 study for antidepressive effects of the agent. A relatively small dose, 150 mg orally for seven days, was employed, and seven of 14 patients responded to the treatment with mild or moderate amelioration of their depressive symptoms. Urinary excretion levels and plasma concentrations of three 5-hydroxyindole compounds, 5-HTP, 5-HT and 5-HIAA, were measured during the drug treatment. Approximately 70% of the orally administered dose of L-5-HTP was recovered from the urine of depressed patients. Major part of urinary indoleamine metabolites was free and conjugate 5-HIAA. Excretion levels of these compounds in urine were not consistently altered in the depressed patients as compared to those in normal subjects. Clinical response to L-5-HTP treatment  appeared to have some correlation with the biochemical measures in the depressed patients, that is, non-responders exhibited significantly lower excretion levels of 5-HT and 5-HIAA in urine, and lower plasma levels of 5-HT than responders. Administered L-5-HTP may not be fully utilized in the depressed patients who did not react to the agent."}
{"id": "PMID:1088161", "title": "Optimal strategies in immunology. I. B-cell differentiation and proliferation.", "content": "The optimal strategy available to the immune system for responding to a non-replicating thymus-independent antigen is examined. By applying Pontryagin's maximum principle to a set of mathematical models of lymphocyte populations and their antibody production, it is found that the optimal strategy of bang-bang control appears robust. In a variety of structurely related biological models, similar behaviour is observed. The models that we consider assume that antigen triggers a population of B-lymphocytes. These triggered lymphocytes can either proliferate and secrete modest amounts of antibody or differentiate into nondividing plasma cells which secrete large amounts of antibody. For biologically reasonable parameter values it is found that for low doses of antigen, immediate differentiation into plasma cells is optimal, while for high antigen doses a proliferative state followed by differentiation is the best strategy.", "contents": "Optimal strategies in immunology. I. B-cell differentiation and proliferation. The optimal strategy available to the immune system for responding to a non-replicating thymus-independent antigen is examined. By applying Pontryagin's maximum principle to a set of mathematical models of lymphocyte populations and their antibody production, it is found that the optimal strategy of bang-bang control appears robust. In a variety of structurely related biological models, similar behaviour is observed. The models that we consider assume that antigen triggers a population of B-lymphocytes. These triggered lymphocytes can either proliferate and secrete modest amounts of antibody or differentiate into nondividing plasma cells which secrete large amounts of antibody. For biologically reasonable parameter values it is found that for low doses of antigen, immediate differentiation into plasma cells is optimal, while for high antigen doses a proliferative state followed by differentiation is the best strategy."}
{"id": "PMID:1088162", "title": "[Concordant deuteranomaly in monozygotic twin sisters (author's transl)].", "content": "Colorblind twin sisters were born from the marriage of a hemizygote with a carrier for colorblindness. The proof of monozygosity is given by the blood-group typing and the dermatoglyphs. The deuteranomaly is of the same degree in each but is more marked in one of the sisters. The mother has none of the microsymptoms sometimes found in carriers.", "contents": "[Concordant deuteranomaly in monozygotic twin sisters (author's transl)]. Colorblind twin sisters were born from the marriage of a hemizygote with a carrier for colorblindness. The proof of monozygosity is given by the blood-group typing and the dermatoglyphs. The deuteranomaly is of the same degree in each but is more marked in one of the sisters. The mother has none of the microsymptoms sometimes found in carriers."}
{"id": "PMID:1088163", "title": "The frequency of 7S rheumatoid factors and 22S complexes in human sera with positive latex tests for rheumatoid factor.", "content": "In sera with positive latex tests for rheumatoid factors, the immunoglobulin class of the antiglobulins is influenced by the quantity of immunoglobulins present in the serum. Sera with 7S antiglobulins have high levels of IgG and IgA immunoglobulins.  22S complexes tend to be found in sera with high levels of IgM immunoglobulins. These findings suggest that the occurrence of antiglobulins and 22S complexes may be influenced by the hypergammaglobulinemia.", "contents": "The frequency of 7S rheumatoid factors and 22S complexes in human sera with positive latex tests for rheumatoid factor. In sera with positive latex tests for rheumatoid factors, the immunoglobulin class of the antiglobulins is influenced by the quantity of immunoglobulins present in the serum. Sera with 7S antiglobulins have high levels of IgG and IgA immunoglobulins.  22S complexes tend to be found in sera with high levels of IgM immunoglobulins. These findings suggest that the occurrence of antiglobulins and 22S complexes may be influenced by the hypergammaglobulinemia."}
{"id": "PMID:1088164", "title": "Restoration of diminished T-cell function in adjuvant induced disease by methotrexate: evidence for two populations of splenic T-cell suppressors.", "content": "Diminished splenic T-cell function, as measured by their response to phytohemagglutinin and Concanavalin-A, was noted only during the active phase of adjuvant induced disease. This diminished function is due to at least two types of suppressor cell: (a) cells which adhere to glass but not plastic, and which are sensitive to methotrexate in vitro; (b) cells which adhere to plastic and glass, and which are relatively insensitive to methotrexate in vitro. Methotrexate treatment in vivo inhibits adjuvant induced disease, and is associated with the absence of both types of suppressor cells in the spleen.", "contents": "Restoration of diminished T-cell function in adjuvant induced disease by methotrexate: evidence for two populations of splenic T-cell suppressors. Diminished splenic T-cell function, as measured by their response to phytohemagglutinin and Concanavalin-A, was noted only during the active phase of adjuvant induced disease. This diminished function is due to at least two types of suppressor cell: (a) cells which adhere to glass but not plastic, and which are sensitive to methotrexate in vitro; (b) cells which adhere to plastic and glass, and which are relatively insensitive to methotrexate in vitro. Methotrexate treatment in vivo inhibits adjuvant induced disease, and is associated with the absence of both types of suppressor cells in the spleen."}
{"id": "PMID:1088165", "title": "Lymphopenia in Sj\u00f6gren's syndrome with rheumatoid arthritis: relationship to lymphocytotoxic antibodies, cryoglobulinemia, and impaired mitogen responsiveness.", "content": "Lymphocyte subpopulation studies in 21 patients with Sj\u00f6gren's syndrome and rheumatoid arthritis revealed an absolute lymphopenia and a normal percentage of T- and SIg-cells. In one patient, a large percentage of lymphocytes bore both IgG and IgM; after cell trypsinization only IgM was resynthesized. This surface IgM was capable of binding human IgG, suggesting that the presence of multiple classes of immunoglobulins on the surface of these lymphocytes was due to surface rheumatoid factor activity. Profound lymphopenia was associated with high concentrations of cryoglobulins and the presence of lymphocytotoxic antibodies. These antibodies were broadly reactive, causing cytotoxicity of T- and SIg-cells from normal subjects, from viral and lymphoproliferative disease subjects, from different organs, and SIg-cells from human lymphoblastoid cell lines. Lymphocyte transformation after phytohemagglutinin and pokeweed mitogen stimulation was impaired in comparison to normal subjects. Warm washing of the lymphocytes and purification to greater than 80 per cent T-cells did not restore mitogen responsiveness to normal, suggesting that cell coating by an antibody and diminished responder cell number were inadequate explanations for the impaired transformation.", "contents": "Lymphopenia in Sj\u00f6gren's syndrome with rheumatoid arthritis: relationship to lymphocytotoxic antibodies, cryoglobulinemia, and impaired mitogen responsiveness. Lymphocyte subpopulation studies in 21 patients with Sj\u00f6gren's syndrome and rheumatoid arthritis revealed an absolute lymphopenia and a normal percentage of T- and SIg-cells. In one patient, a large percentage of lymphocytes bore both IgG and IgM; after cell trypsinization only IgM was resynthesized. This surface IgM was capable of binding human IgG, suggesting that the presence of multiple classes of immunoglobulins on the surface of these lymphocytes was due to surface rheumatoid factor activity. Profound lymphopenia was associated with high concentrations of cryoglobulins and the presence of lymphocytotoxic antibodies. These antibodies were broadly reactive, causing cytotoxicity of T- and SIg-cells from normal subjects, from viral and lymphoproliferative disease subjects, from different organs, and SIg-cells from human lymphoblastoid cell lines. Lymphocyte transformation after phytohemagglutinin and pokeweed mitogen stimulation was impaired in comparison to normal subjects. Warm washing of the lymphocytes and purification to greater than 80 per cent T-cells did not restore mitogen responsiveness to normal, suggesting that cell coating by an antibody and diminished responder cell number were inadequate explanations for the impaired transformation."}
{"id": "PMID:1088166", "title": "Therapeutic trials with levamisole and other agents in NZB/W mice.", "content": "The effects of levamisole, ribovirin, and cyclophosphamide in preventing the spontaneous autoimmune disease of NZB/W mice have been evaluated. These drugs all had a significant effect, both in delaying mortality, and in postponing the development of antinuclear antibodies and proteinuria. Single-stranded DNA linked to IgG was also used but had no demonstrable effect. The results of therapeutic studies in murine lupus must be interpreted with caution in relation to the human disease, but as both levamisole and ribovirin are now being used in man, our results suggest that further studies with these drugs are warranted.", "contents": "Therapeutic trials with levamisole and other agents in NZB/W mice. The effects of levamisole, ribovirin, and cyclophosphamide in preventing the spontaneous autoimmune disease of NZB/W mice have been evaluated. These drugs all had a significant effect, both in delaying mortality, and in postponing the development of antinuclear antibodies and proteinuria. Single-stranded DNA linked to IgG was also used but had no demonstrable effect. The results of therapeutic studies in murine lupus must be interpreted with caution in relation to the human disease, but as both levamisole and ribovirin are now being used in man, our results suggest that further studies with these drugs are warranted."}
{"id": "PMID:1088168", "title": "Effects of metabolic inhibitors and ouabain on static afferent discharges from the isolated frog muscle spindle.", "content": "Effects of metabolic inhibitors and ouabain on the rate of afferent discharges from isolated muscle spindle of the bullfrog were examined in vitro to elucidate the mechanism for generating afferent action potentials. Dinitrophenol, NaCN, iodoacetic acid, and ouabain gradually reduced the rate of discharges. These drugs were without direct effect on the nerve axon. The rate of discharges was also reduced by Li+, which is known to be minimally extruded from the interior of cells by an active transport mechanism. The rate of discharges remained approximately 50% in the presence of NaCN or in the absence of oxygen, suggesting that the energy required for the maintenance of the Na+-pump can be supplied to some extent in an anaerobic condition. It appears that the metabolic inhibitors and ouabain reduce the afferent discharges from the frog muscle spindle by affecting the Na+-pump in fibers of nerve terminals.", "contents": "Effects of metabolic inhibitors and ouabain on static afferent discharges from the isolated frog muscle spindle. Effects of metabolic inhibitors and ouabain on the rate of afferent discharges from isolated muscle spindle of the bullfrog were examined in vitro to elucidate the mechanism for generating afferent action potentials. Dinitrophenol, NaCN, iodoacetic acid, and ouabain gradually reduced the rate of discharges. These drugs were without direct effect on the nerve axon. The rate of discharges was also reduced by Li+, which is known to be minimally extruded from the interior of cells by an active transport mechanism. The rate of discharges remained approximately 50% in the presence of NaCN or in the absence of oxygen, suggesting that the energy required for the maintenance of the Na+-pump can be supplied to some extent in an anaerobic condition. It appears that the metabolic inhibitors and ouabain reduce the afferent discharges from the frog muscle spindle by affecting the Na+-pump in fibers of nerve terminals."}
{"id": "PMID:1088212", "title": "Significance of T and B lymphocytes in different chronic internal diseases with immune processes.", "content": "The T and B lymphocytes were determined by the rosette test in the peripheral blood of 100 patients with internal chronic diseases with immune processes such as: chronic hepatitis, systemic lupus erythematosus, myasthenia gravis, chronic lymphocytic leukemia and lymphosarcoma. The values obtained were compared with those in 10 controls. The mean per cent values of the T lymphocytes in the controls were 33 (29-38%) and of the B lymphocytes 25.2 (16-43%). In the patients investigated the values varied, namely: in chronic hepatitis and systemic lupus erythematosus the T lymphocytes were generally low while the B lymphocytes were moderately high. In myasthenia gravis both T and B lymphocytes were below the control values. In chronic lymphocytic leukemias and lymphosarcomas the per cent values of the T lymphocytes were generally low while those of the B lymphocytes were high. The significance of these results for the immunologic implications of the lymphocyte populations is discussed and an attempt is made to correlate these data with the nucleologram variations.", "contents": "Significance of T and B lymphocytes in different chronic internal diseases with immune processes. The T and B lymphocytes were determined by the rosette test in the peripheral blood of 100 patients with internal chronic diseases with immune processes such as: chronic hepatitis, systemic lupus erythematosus, myasthenia gravis, chronic lymphocytic leukemia and lymphosarcoma. The values obtained were compared with those in 10 controls. The mean per cent values of the T lymphocytes in the controls were 33 (29-38%) and of the B lymphocytes 25.2 (16-43%). In the patients investigated the values varied, namely: in chronic hepatitis and systemic lupus erythematosus the T lymphocytes were generally low while the B lymphocytes were moderately high. In myasthenia gravis both T and B lymphocytes were below the control values. In chronic lymphocytic leukemias and lymphosarcomas the per cent values of the T lymphocytes were generally low while those of the B lymphocytes were high. The significance of these results for the immunologic implications of the lymphocyte populations is discussed and an attempt is made to correlate these data with the nucleologram variations."}
{"id": "PMID:1088229", "title": "[Effect of sensory and sympathetic denervation of the frog tongue on the catecholamine containing cells of the taste buds].", "content": "The structure of catecholamine-containing dumb-bell shaped cells of the taste buds was studied by luminescent microscopy in the epithelial layer of the frog's tongue (Rana temporaria). On the unilateral section of the lingual nerve, a maintained adrenergic innervation of vessels and of the epithelium was observed, a decreased number of dumb-bell shaped cells in the taste bud, and their significant enlargement, and increased cathecholamine luminescence. With desympathization, no adrenergic nerves were observed on the vessels and the epithelium of the tongue. The size of the taste buds in desympathized cells of the tongue is sharply decreased and their number is increased. There is a tendency to grouping of the dumbbell shaped cells into 3--4 taste buds in one fungiform papillina. The experiments with sensory and sympathetic denervation of the frog tongue distinctly showed the trophic action of sensory and sympathetic nerves on the taste organ of the frog.", "contents": "[Effect of sensory and sympathetic denervation of the frog tongue on the catecholamine containing cells of the taste buds]. The structure of catecholamine-containing dumb-bell shaped cells of the taste buds was studied by luminescent microscopy in the epithelial layer of the frog's tongue (Rana temporaria). On the unilateral section of the lingual nerve, a maintained adrenergic innervation of vessels and of the epithelium was observed, a decreased number of dumb-bell shaped cells in the taste bud, and their significant enlargement, and increased cathecholamine luminescence. With desympathization, no adrenergic nerves were observed on the vessels and the epithelium of the tongue. The size of the taste buds in desympathized cells of the tongue is sharply decreased and their number is increased. There is a tendency to grouping of the dumbbell shaped cells into 3--4 taste buds in one fungiform papillina. The experiments with sensory and sympathetic denervation of the frog tongue distinctly showed the trophic action of sensory and sympathetic nerves on the taste organ of the frog."}
{"id": "PMID:1088230", "title": "[Mechanisms of action of synthetic polyelectrolytes and polyampholytes on the immune response].", "content": "We investigated the effect of polyacrilic acid (PAA) on the immune response in mice of various strains on sheep red blood cells and also the influence of poly-2-methyl-5-vinyl-pyridine (PMVY), PAA and their statistical copolymers on antibody-forming cells (AFC) production in cultures of T- and B-lymphocytes in vivo. PAA was seen to increase accumulation of AFC in the spleen of mice depending on their genotypes. PMVP and PAA were found to intensify the cooperating interaction of T- and B-lymphocytes, whereas their copolymers exert quite an opposite effect. The injection of copolymers to the recipients of cooperating T- and B-lymphocytes practically results in the complete elimination of the cooperation effect between T- and B-lymphocytes in the immune response to sheep erythrocytes without cytostatic action of cell proliferation.", "contents": "[Mechanisms of action of synthetic polyelectrolytes and polyampholytes on the immune response]. We investigated the effect of polyacrilic acid (PAA) on the immune response in mice of various strains on sheep red blood cells and also the influence of poly-2-methyl-5-vinyl-pyridine (PMVY), PAA and their statistical copolymers on antibody-forming cells (AFC) production in cultures of T- and B-lymphocytes in vivo. PAA was seen to increase accumulation of AFC in the spleen of mice depending on their genotypes. PMVP and PAA were found to intensify the cooperating interaction of T- and B-lymphocytes, whereas their copolymers exert quite an opposite effect. The injection of copolymers to the recipients of cooperating T- and B-lymphocytes practically results in the complete elimination of the cooperation effect between T- and B-lymphocytes in the immune response to sheep erythrocytes without cytostatic action of cell proliferation."}
{"id": "PMID:1088241", "title": "Myasthenia gravis: mitotic activity of thymus-dependent and blood-lymphocytes.", "content": "The thymus and blood lymphocytes of 20 thymectomized patients with myasthenia gravis, including 3 cases of thymoma, have been studied for mitotic activity (spontaneous mitotic activity of native thymocytes, responsiveness of cultured cells to PHA, DNA-polymerase specific activity). The myasthenic thymus gland was found to be the site of a spontaneous DNA-synthesis of similar intensity as the mitotic activity of the thymus in infancy. This was also reflected by the DNA-polymerase activity of the cells. While the thymocyte cultures responded well to PHA stimulation in 50% of the cases, the blood-lymphocytes were found unresponsive. There was a partial correlation between the individual parameters of mitotic activity, but practically none between the mitotic activity and the clinical severity of myasthenia or the histological features of the thymus or of thymoma. Except in the latter cases, a correlation was found between mitotic activity and preoperative immunosuppressive treatment. It is suggested that the T-lymphocytes are not directly involved in the pathomechanism of myasthenia gravis.", "contents": "Myasthenia gravis: mitotic activity of thymus-dependent and blood-lymphocytes. The thymus and blood lymphocytes of 20 thymectomized patients with myasthenia gravis, including 3 cases of thymoma, have been studied for mitotic activity (spontaneous mitotic activity of native thymocytes, responsiveness of cultured cells to PHA, DNA-polymerase specific activity). The myasthenic thymus gland was found to be the site of a spontaneous DNA-synthesis of similar intensity as the mitotic activity of the thymus in infancy. This was also reflected by the DNA-polymerase activity of the cells. While the thymocyte cultures responded well to PHA stimulation in 50% of the cases, the blood-lymphocytes were found unresponsive. There was a partial correlation between the individual parameters of mitotic activity, but practically none between the mitotic activity and the clinical severity of myasthenia or the histological features of the thymus or of thymoma. Except in the latter cases, a correlation was found between mitotic activity and preoperative immunosuppressive treatment. It is suggested that the T-lymphocytes are not directly involved in the pathomechanism of myasthenia gravis."}
{"id": "PMID:1088242", "title": "Cellular immune reactivity to nuclear antigens in systemic lupus erythematosus.", "content": "The leucocyte migration test (LMT) was used for the study of cellular responsiveness to nuclear factors, i. e. to calf thymus desoxyribonucleic acid (DNA), calf thymus desoxyribonucleoprotein (DNP), calf liver ribonucleic acid (RNA), in 20 subjects with SLE and in 20 non-immunopathological cases serving as controls. The nucleic acid preparations were found to produce a positive LMT in the SLE-patients as well as in the controls. The proportion of positive responses reached its highest percentage in the cases of active SLE. Two cases were followed up by repeated LMT in the course of immunosuppressive treatment with cytostatics and/or antilymphocyte globulin (ALG) administration indicated by the activity of the process. The LMT proved suitable for assessing the intensity of immunosuppression by cytostatics or by ALG. A critical evaluation of the procedure is given. It is pointed out that inhibition and stimulation of migration are likewise manifestations of cellular immune responsiveness. Both inhibition and stimulation are more marked if serum-free nutrient is used.", "contents": "Cellular immune reactivity to nuclear antigens in systemic lupus erythematosus. The leucocyte migration test (LMT) was used for the study of cellular responsiveness to nuclear factors, i. e. to calf thymus desoxyribonucleic acid (DNA), calf thymus desoxyribonucleoprotein (DNP), calf liver ribonucleic acid (RNA), in 20 subjects with SLE and in 20 non-immunopathological cases serving as controls. The nucleic acid preparations were found to produce a positive LMT in the SLE-patients as well as in the controls. The proportion of positive responses reached its highest percentage in the cases of active SLE. Two cases were followed up by repeated LMT in the course of immunosuppressive treatment with cytostatics and/or antilymphocyte globulin (ALG) administration indicated by the activity of the process. The LMT proved suitable for assessing the intensity of immunosuppression by cytostatics or by ALG. A critical evaluation of the procedure is given. It is pointed out that inhibition and stimulation of migration are likewise manifestations of cellular immune responsiveness. Both inhibition and stimulation are more marked if serum-free nutrient is used."}
{"id": "PMID:1088244", "title": "Functional significance of the ventral and lateral funiculi in the frog's spinal cord.", "content": "The spinal cord was transected at the level of calamus scriptorius either completely (spinal preparation) or partially (funicular preparation). In the operated frogs and in normal controls cranial and peripheral receptive fields were stimulated by natural stimuli. Results indicated that the information necessary for somatic activation of the protective flight ascends in the dorsolateral funiculus, which can be characterized as an ascending part of the reflex arc of somatotopically guided avoidance behavior. The tectal region is apparently a center of responses. The information necessary for activating different patterns of locomotion adequate to environmental conditions goes unilaterally through the ventral and ventrolateral funiculus. A diminished specificity of these motor patterns observed after injury of the ventrolateral funiculus seems to be related to a reduced feedback from the periphery. Lacking of aquatic pattern of limb movements (even when terrestrial patterns of locomotion were undisturbed), and drowning in water seem to be related to an injury of a vestibulo-spinal system in the ventral funiculus. It is concluded that the ventrolateral area of the frog spinal cord, i.e., the area between median fissure and dorsal root, forms a closed system providing afferent and efferent information sufficient to evoke normal behavior.", "contents": "Functional significance of the ventral and lateral funiculi in the frog's spinal cord. The spinal cord was transected at the level of calamus scriptorius either completely (spinal preparation) or partially (funicular preparation). In the operated frogs and in normal controls cranial and peripheral receptive fields were stimulated by natural stimuli. Results indicated that the information necessary for somatic activation of the protective flight ascends in the dorsolateral funiculus, which can be characterized as an ascending part of the reflex arc of somatotopically guided avoidance behavior. The tectal region is apparently a center of responses. The information necessary for activating different patterns of locomotion adequate to environmental conditions goes unilaterally through the ventral and ventrolateral funiculus. A diminished specificity of these motor patterns observed after injury of the ventrolateral funiculus seems to be related to a reduced feedback from the periphery. Lacking of aquatic pattern of limb movements (even when terrestrial patterns of locomotion were undisturbed), and drowning in water seem to be related to an injury of a vestibulo-spinal system in the ventral funiculus. It is concluded that the ventrolateral area of the frog spinal cord, i.e., the area between median fissure and dorsal root, forms a closed system providing afferent and efferent information sufficient to evoke normal behavior."}
{"id": "PMID:1088246", "title": "Approaches to selectivity in cancer chemotherapy.", "content": "Most chemotherapeutic agents utilized in the treatment of cancer have toxic effects, not only on tumor cells but also on the normal replicating cells of the body. Some of these toxic effects on the normal tissues by the drug may be diminished, or more promising is the utilization of more selective drugs based on differences between the normal and the neoplastic cell.", "contents": "Approaches to selectivity in cancer chemotherapy. Most chemotherapeutic agents utilized in the treatment of cancer have toxic effects, not only on tumor cells but also on the normal replicating cells of the body. Some of these toxic effects on the normal tissues by the drug may be diminished, or more promising is the utilization of more selective drugs based on differences between the normal and the neoplastic cell."}
{"id": "PMID:1088249", "title": "[The genetics of the protease inhibitor system and osteospongiosis. Statistical study in 199 patients].", "content": "Since the trypsin activity of the inner ear fluid appears to be essential in the evolution of otosclerosis towards a cochlear deafness it was obvious that the protease inhibitor system which is an excellent genetic marker ought to be studied during the onset and the evolution of otosclerosis. No differences with the statistical data from the normal population was found the origin of the trypsin that destroys the inner ear is to be found locally the histiocytes of the otosclerosis focus.", "contents": "[The genetics of the protease inhibitor system and osteospongiosis. Statistical study in 199 patients]. Since the trypsin activity of the inner ear fluid appears to be essential in the evolution of otosclerosis towards a cochlear deafness it was obvious that the protease inhibitor system which is an excellent genetic marker ought to be studied during the onset and the evolution of otosclerosis. No differences with the statistical data from the normal population was found the origin of the trypsin that destroys the inner ear is to be found locally the histiocytes of the otosclerosis focus."}
{"id": "PMID:1088251", "title": "[Characteristics of epidemiology and pathomorphosis of tuberculosis at the present-day stage].", "content": "The article deals with data confirming changes in epidemiology of tuberculosis and its pathomorphism during the last years. A considerable decrease in the incidence rate among children, adolescents, young people is noted. Prevalence of tuberculosis and death rate have also dropped. Primary tuberculosis in children most often takes a form of minor bronchoadenitis, and in adults -- a form of a pulmonary process. As a result of natural and induced pathomorphism pulmonary tuberculosis in adults, particulary in old age, is accompanied by secondary nonspecific changes in various organs, which with years become more important in thanatogenesis.", "contents": "[Characteristics of epidemiology and pathomorphosis of tuberculosis at the present-day stage]. The article deals with data confirming changes in epidemiology of tuberculosis and its pathomorphism during the last years. A considerable decrease in the incidence rate among children, adolescents, young people is noted. Prevalence of tuberculosis and death rate have also dropped. Primary tuberculosis in children most often takes a form of minor bronchoadenitis, and in adults -- a form of a pulmonary process. As a result of natural and induced pathomorphism pulmonary tuberculosis in adults, particulary in old age, is accompanied by secondary nonspecific changes in various organs, which with years become more important in thanatogenesis."}
{"id": "PMID:1088261", "title": "The use of 5-hydroxytryptophan in a child with Lesch-Nyhan syndrome.", "content": "The effects of 5-hydroxytryptophan (5-HTP), a serotonin precursor, were studied in a boy with the Lesch-Nyhan syndrome. During the course of drug treatment, self-mutilation, crying, sleep state architecture, serum dopamine B-hydroxylase (DBH), and cerebrospinal fluid levels of 5-hydroxyindolylacetic acid and homovanillic acid were studied. Treatment with 5-HTP failed to affect this child's biting behavior. However, the drug significantly reduced irritability as measured by crying time. Moreover, 5-HTP may have affected sleep state architecture, making it more normal in character. Serum DBH levels were normal throughout the study. Neither the patient's mother nor his maternal grandmother showed a hypertensive response on the cold pressor test.", "contents": "The use of 5-hydroxytryptophan in a child with Lesch-Nyhan syndrome. The effects of 5-hydroxytryptophan (5-HTP), a serotonin precursor, were studied in a boy with the Lesch-Nyhan syndrome. During the course of drug treatment, self-mutilation, crying, sleep state architecture, serum dopamine B-hydroxylase (DBH), and cerebrospinal fluid levels of 5-hydroxyindolylacetic acid and homovanillic acid were studied. Treatment with 5-HTP failed to affect this child's biting behavior. However, the drug significantly reduced irritability as measured by crying time. Moreover, 5-HTP may have affected sleep state architecture, making it more normal in character. Serum DBH levels were normal throughout the study. Neither the patient's mother nor his maternal grandmother showed a hypertensive response on the cold pressor test."}
{"id": "PMID:1088263", "title": "[Pharmacologic activity of pyrimidoindole derivatives].", "content": "The spectrum of the psychotropic activity of a number of new pyrimidoindole derivatives and the relation between their chemical structure and activity were studied experimentally. The research was done on mice by employing tests usually applied in estimating neuroleptics and antidepressants of tricyclic structure. The study of indole derivatives are shown to display a sedative action. 5-methyl derivatives of pyrimido- and tetrahydropyrimido [3,4-a] indole without any substituent in the 2nd position are 5-oxytryptophan antagonists. The 2,5-dimethyltetrahydropyrimido [3,4-a] indole derivatives electively potentiate the central effect of 5-oxytryptophan and display in experiments a specific spectrum of the pharmacological action, resembling antidepressants by a number of tests. As concerns their activity these compounds, however, are inferior to amitriptyline and pyrasidol.", "contents": "[Pharmacologic activity of pyrimidoindole derivatives]. The spectrum of the psychotropic activity of a number of new pyrimidoindole derivatives and the relation between their chemical structure and activity were studied experimentally. The research was done on mice by employing tests usually applied in estimating neuroleptics and antidepressants of tricyclic structure. The study of indole derivatives are shown to display a sedative action. 5-methyl derivatives of pyrimido- and tetrahydropyrimido [3,4-a] indole without any substituent in the 2nd position are 5-oxytryptophan antagonists. The 2,5-dimethyltetrahydropyrimido [3,4-a] indole derivatives electively potentiate the central effect of 5-oxytryptophan and display in experiments a specific spectrum of the pharmacological action, resembling antidepressants by a number of tests. As concerns their activity these compounds, however, are inferior to amitriptyline and pyrasidol."}
{"id": "PMID:1088267", "title": "Handling of induced hypercalcaemia in children with rheumatic and rheumatoid affections.", "content": "Induced hypercalcemia in rheumatic and rheumatoid children revealed the following : 1. Rheumatic and rheumatoid patients deal more effectively with induced hypercalcemia than control children. 2. A higher percentage of infused calcium in these patients is retained. This may by due to higher calcitionin activity relative to controls. 3. Induced hypercalcemia resulted in an increase in urinary 17 OH C.S. This is suggested to be due to stimulation of the adrenal cortex. These findings suggest that calcium infusion is an effective additional measure in the treatment of rheumatic and rheumatoid affection in the absence of contraindication to calcium.", "contents": "Handling of induced hypercalcaemia in children with rheumatic and rheumatoid affections. Induced hypercalcemia in rheumatic and rheumatoid children revealed the following : 1. Rheumatic and rheumatoid patients deal more effectively with induced hypercalcemia than control children. 2. A higher percentage of infused calcium in these patients is retained. This may by due to higher calcitionin activity relative to controls. 3. Induced hypercalcemia resulted in an increase in urinary 17 OH C.S. This is suggested to be due to stimulation of the adrenal cortex. These findings suggest that calcium infusion is an effective additional measure in the treatment of rheumatic and rheumatoid affection in the absence of contraindication to calcium."}
{"id": "PMID:1088284", "title": "Incorporation of newly formed lecithin into peripheral nerve myelin.", "content": "Radioactive choline was used to study the metabolism and movement of choline-containing phospholipids in peripheral nerve myelin of adult mice. Incorporation at various times after intraperitoneal injection was measured in serial segments of sciatic nerve as well as in myelin isolated from those segments. At no time (1 h to 35 days) could a proximal-distal difference in the extent of labeling be demonstrated. This finding suggests that incorporation of precursor choline phospholipids into nerve membranes is a local event, with little contribution from the neuronal perikaryon via axoplasmic transport. Autoradiographic investigations were undertaken to elucidate the pattern of movement of radioactive choline-labeled phospholipids, predominantly lecithin, into the myelin sheaths of the sciatic nerve. A sequence of autoradiographs was prepared from animals sacrificed between 20 min and 35 days after a microinjection of precursor directly into the nerve. Analysis of these autoradiograms revealed that labeling is initially concentrated in the Schwann cell cytoplasm. Later, the label moves first into the outer regions of the myelin sheaths and is eventually distributed evenly throughout the inner and outer layers of the sheath. At no time is there a build-up of label in the axon. The rate of uptake of precursor and subsequent redistribution of lecithin into the myelin were also examined in frog sciatic nerve (18 degrees C). Both uptake and redistribution processes were considerably slower in the cold-blooded animal.", "contents": "Incorporation of newly formed lecithin into peripheral nerve myelin. Radioactive choline was used to study the metabolism and movement of choline-containing phospholipids in peripheral nerve myelin of adult mice. Incorporation at various times after intraperitoneal injection was measured in serial segments of sciatic nerve as well as in myelin isolated from those segments. At no time (1 h to 35 days) could a proximal-distal difference in the extent of labeling be demonstrated. This finding suggests that incorporation of precursor choline phospholipids into nerve membranes is a local event, with little contribution from the neuronal perikaryon via axoplasmic transport. Autoradiographic investigations were undertaken to elucidate the pattern of movement of radioactive choline-labeled phospholipids, predominantly lecithin, into the myelin sheaths of the sciatic nerve. A sequence of autoradiographs was prepared from animals sacrificed between 20 min and 35 days after a microinjection of precursor directly into the nerve. Analysis of these autoradiograms revealed that labeling is initially concentrated in the Schwann cell cytoplasm. Later, the label moves first into the outer regions of the myelin sheaths and is eventually distributed evenly throughout the inner and outer layers of the sheath. At no time is there a build-up of label in the axon. The rate of uptake of precursor and subsequent redistribution of lecithin into the myelin were also examined in frog sciatic nerve (18 degrees C). Both uptake and redistribution processes were considerably slower in the cold-blooded animal."}
{"id": "PMID:1088285", "title": "Control of chromosome behavior in amphibian oocytes. II. The effect of inhibitors of RNA and protein synthesis on the induction of chromosome condensation in transplanted brain nuclei by oocyte cytoplasm.", "content": "We studied the effects of actinomycin D, alpha-amanitin, puromycin, and cycloheximide on the cytoplasmic activity of maturing Rana pipiens oocytes that induces chromosome condensation in transplanted brain nuclei. Treatment of oocytes with each inhibitor suppressed the chromosome condensation induced by metaphase oocytes to varying degrees depending upon the dose of inhibitor, despite the fact that untreated metaphase I oocytes already possessed chromosome condensation activity (CCA). Treatment of brain nuclei before injection completely suppressed condensation at all doses used. Chromosome condensation induced by metaphase II oocyte cytoplasm, however, was insensitive to all the inhibitors, even when the brain nuclei were pretreated. Oocytes treated with alpha-amanitin throughout maturation induced chromosome condensation when tested at metaphase II. Removal of the oocyte chromosomes after the germinal vesicle (GV) broke down did not prevent the development of CCA, whereas removal of the entire GV before initiation of maturation deprived oocytes of CCA. The results suggest that metaphase I oocyte cytoplasm stimulates synthesis of brain nuclear RNAs that are translated into proteins necessary for chromosome condensation, whereas metaphase II oocytes possess all the factors for chromosome condensation. In both cases, GV nucleoplasm appears indispensable for the development of CCA, whereas immediate activity of the oocyte genome is not required.", "contents": "Control of chromosome behavior in amphibian oocytes. II. The effect of inhibitors of RNA and protein synthesis on the induction of chromosome condensation in transplanted brain nuclei by oocyte cytoplasm. We studied the effects of actinomycin D, alpha-amanitin, puromycin, and cycloheximide on the cytoplasmic activity of maturing Rana pipiens oocytes that induces chromosome condensation in transplanted brain nuclei. Treatment of oocytes with each inhibitor suppressed the chromosome condensation induced by metaphase oocytes to varying degrees depending upon the dose of inhibitor, despite the fact that untreated metaphase I oocytes already possessed chromosome condensation activity (CCA). Treatment of brain nuclei before injection completely suppressed condensation at all doses used. Chromosome condensation induced by metaphase II oocyte cytoplasm, however, was insensitive to all the inhibitors, even when the brain nuclei were pretreated. Oocytes treated with alpha-amanitin throughout maturation induced chromosome condensation when tested at metaphase II. Removal of the oocyte chromosomes after the germinal vesicle (GV) broke down did not prevent the development of CCA, whereas removal of the entire GV before initiation of maturation deprived oocytes of CCA. The results suggest that metaphase I oocyte cytoplasm stimulates synthesis of brain nuclear RNAs that are translated into proteins necessary for chromosome condensation, whereas metaphase II oocytes possess all the factors for chromosome condensation. In both cases, GV nucleoplasm appears indispensable for the development of CCA, whereas immediate activity of the oocyte genome is not required."}
{"id": "PMID:1088287", "title": "Verruca plantaris: a review of the problem.", "content": "Verruca plantaris, caused by papovavirus, is frequently seen in patients between the ages of 12 and 16 years. Some type of tissue destruction is used in most methods of treatment. The author discusses electrodesiccation which the considers the treatment of choice as excellent cosmetic results are usually obtained and recurrences are minimal.", "contents": "Verruca plantaris: a review of the problem. Verruca plantaris, caused by papovavirus, is frequently seen in patients between the ages of 12 and 16 years. Some type of tissue destruction is used in most methods of treatment. The author discusses electrodesiccation which the considers the treatment of choice as excellent cosmetic results are usually obtained and recurrences are minimal."}
{"id": "PMID:1088292", "title": "Immunogenicity of lysozyme derivatives lipid-conjugated to various degrees in mice treated with and without cyclophosphamide: dissociation of delayed-type hypersensitivity and helper function.", "content": "Lysozyme and a series of its lipid-conjugated derivatives without adjuvant were examined in mice for their abilities to induce delayed-type hypersensitivity (DTH), helper T-cell activity, and antibody formation. In addition, the effect of cyclophosphamide (CY) on the immune responses was assessed in mice immunized with these lysozyme derivatives. Precipitated lysozyme without lipid conjugation was a good inducer of both antibody and DTH responses. Lipid conjugation to lysozyme to intermediate degrees readily caused the failure only in inducing the antibody response. As lysozyme was lipid-conjugated more heavily, DTH response was also reduced and finally abolished. In contrast, the helper activity was little affected by any degree of lipid conjugation. These results indicate that the helper T-cell activity was dissociated from the both DTH response and the antibody production. CY pretreatment extensively enhanced DTH response induced by such lipid-conjugated derivatives that failed to induce antibody response. Furthermore, CY pretreatment in doses in a wide range enhanced not only DTH response but also antibody formation. It is, therefore, concluded that the enhancement of DTH response by CY does not necessarily entail suppression of antibody formation.", "contents": "Immunogenicity of lysozyme derivatives lipid-conjugated to various degrees in mice treated with and without cyclophosphamide: dissociation of delayed-type hypersensitivity and helper function. Lysozyme and a series of its lipid-conjugated derivatives without adjuvant were examined in mice for their abilities to induce delayed-type hypersensitivity (DTH), helper T-cell activity, and antibody formation. In addition, the effect of cyclophosphamide (CY) on the immune responses was assessed in mice immunized with these lysozyme derivatives. Precipitated lysozyme without lipid conjugation was a good inducer of both antibody and DTH responses. Lipid conjugation to lysozyme to intermediate degrees readily caused the failure only in inducing the antibody response. As lysozyme was lipid-conjugated more heavily, DTH response was also reduced and finally abolished. In contrast, the helper activity was little affected by any degree of lipid conjugation. These results indicate that the helper T-cell activity was dissociated from the both DTH response and the antibody production. CY pretreatment extensively enhanced DTH response induced by such lipid-conjugated derivatives that failed to induce antibody response. Furthermore, CY pretreatment in doses in a wide range enhanced not only DTH response but also antibody formation. It is, therefore, concluded that the enhancement of DTH response by CY does not necessarily entail suppression of antibody formation."}
{"id": "PMID:1088293", "title": "Analysis of the slow excitatory postsynaptic potential in bullfrog sympathetic ganglion cells.", "content": "The ionic mechanism of the slow excitatory postsynaptic potential (slow EPSP), i.e. the muscarinic action of acetylcholine (ACh), was studied either by stimulating preganglionic nerves or by applying ACh in curarized sympathetic ganglion cells of bullfrogs. There are three different types of cells characterized by the effects of membrane hyperpoliarization on the amplitude of slow EPSP. One group of cells showed an increase in amplitude (type 1 cell) and, in two other groups of cells, it remained unchanged (type 2 cell) or decreased (type 3 cell), when the membrane was hyperpolarized. Under the muscarinic effects of ACh, the slope membrane conductance was increased (type 1 cell), unchanged (type 2 cell) or decreased (type 3 cell) at 10-20 mV hyperpolarized levels, while it was unchanged (type 1 cell) or decreased (types 2 and 3 cells) at resting and depolarized levels. In all cells, the slow ACh potential, corresponding to the slow EPSP, was almost completely suppressed in a high K+, Ca2+-free, Na+-free solution. These results suggest that the slow EPSP is generated by increases in Na+ and Ca2+ conductance and also by a simultaneous decrease in the K+ conductance.", "contents": "Analysis of the slow excitatory postsynaptic potential in bullfrog sympathetic ganglion cells. The ionic mechanism of the slow excitatory postsynaptic potential (slow EPSP), i.e. the muscarinic action of acetylcholine (ACh), was studied either by stimulating preganglionic nerves or by applying ACh in curarized sympathetic ganglion cells of bullfrogs. There are three different types of cells characterized by the effects of membrane hyperpoliarization on the amplitude of slow EPSP. One group of cells showed an increase in amplitude (type 1 cell) and, in two other groups of cells, it remained unchanged (type 2 cell) or decreased (type 3 cell), when the membrane was hyperpolarized. Under the muscarinic effects of ACh, the slope membrane conductance was increased (type 1 cell), unchanged (type 2 cell) or decreased (type 3 cell) at 10-20 mV hyperpolarized levels, while it was unchanged (type 1 cell) or decreased (types 2 and 3 cells) at resting and depolarized levels. In all cells, the slow ACh potential, corresponding to the slow EPSP, was almost completely suppressed in a high K+, Ca2+-free, Na+-free solution. These results suggest that the slow EPSP is generated by increases in Na+ and Ca2+ conductance and also by a simultaneous decrease in the K+ conductance."}
{"id": "PMID:1088294", "title": "The muscarinic effects of acetylcholine on the action potential of bullfrog sympathetic ganglion cells.", "content": "The direct effects of acetylcholine (ACh) on Na+- or Ca2+-dependent action potentials of curarized sympathetic ganglion cells in bullfrogs were investigated under a condition where membrane depolarization caused by the muscarinic action of ACh was nullified by means of a hyperpolarizing current. ACh decreased the after-hyperpolarization of Na+-action potentials in Ringer's solution, and increased the after-depolarization of Ca2+-action potentials in the isotonic Ca2+ solution. In both solutions, the maximum rates of rise of the spikes were decreased and the slope membrane resistance at the original resting level was increased. The effects of ACh were abolished by atropine. On the other hand, ACh showed no significant effects on action potentials of bullfrog spinal ganglion cells which possessed no synapses. These results suggest that the ion conductance channels for generation of action potentials of sympathetic ganglion cells are under the direct control of transmitters, such as ACh.", "contents": "The muscarinic effects of acetylcholine on the action potential of bullfrog sympathetic ganglion cells. The direct effects of acetylcholine (ACh) on Na+- or Ca2+-dependent action potentials of curarized sympathetic ganglion cells in bullfrogs were investigated under a condition where membrane depolarization caused by the muscarinic action of ACh was nullified by means of a hyperpolarizing current. ACh decreased the after-hyperpolarization of Na+-action potentials in Ringer's solution, and increased the after-depolarization of Ca2+-action potentials in the isotonic Ca2+ solution. In both solutions, the maximum rates of rise of the spikes were decreased and the slope membrane resistance at the original resting level was increased. The effects of ACh were abolished by atropine. On the other hand, ACh showed no significant effects on action potentials of bullfrog spinal ganglion cells which possessed no synapses. These results suggest that the ion conductance channels for generation of action potentials of sympathetic ganglion cells are under the direct control of transmitters, such as ACh."}
{"id": "PMID:1088297", "title": "Spontaneous regression of sarcoma.", "content": "Three pathologic processes generally regarded as nonneoplastic conditions are presented and discussed as examples of poorly controlled proliferations of lymphoid tissue. A study of these three disorders (infectious mononucleosis, hyperthyroidism, and Dilantin-induced lymphadenopathy) provides clues to an understanding of the pathogenesis and occasional spontaneous regression of the malignant lymphomas.", "contents": "Spontaneous regression of sarcoma. Three pathologic processes generally regarded as nonneoplastic conditions are presented and discussed as examples of poorly controlled proliferations of lymphoid tissue. A study of these three disorders (infectious mononucleosis, hyperthyroidism, and Dilantin-induced lymphadenopathy) provides clues to an understanding of the pathogenesis and occasional spontaneous regression of the malignant lymphomas."}
{"id": "PMID:1088302", "title": "An unexpected effect of L-5 hydroxytryptophan-ethyl-ester combined with a peripheral decarboxylase inhibitor on human serum prolactin.", "content": "While the 5-HT precursors tryptophan and 1-5-HTP cause an increase in serum prolactin concentration, a combination of 1-5-HTP with a peripheral decarboxylase inhibitor was found to reduce the serum prolactin concentration. This combination seemed to behave like a DA agonist. This effect is not produced by the decarboxylase inhibitor per se. A possible explanation is that 5-HTP is converted to 5-HT in CA-ergic neurons, that 5-HT supersedes the CA from the stores, and that some of the CA reach the synaptic cleft and stimulate CA receptors. Another possible explanation is that 5-HTP decarboxylase is centrally inhibited as well, and that an effect of 5-HTP itself is involved here. In view of the observations made it is doubtful whether the therapeutic effect of 5-HTP combined with a peripheral decarboxylase inhibitor in depressions and myoclonus can in fact be atributed to activation of central serotonergic systems.", "contents": "An unexpected effect of L-5 hydroxytryptophan-ethyl-ester combined with a peripheral decarboxylase inhibitor on human serum prolactin. While the 5-HT precursors tryptophan and 1-5-HTP cause an increase in serum prolactin concentration, a combination of 1-5-HTP with a peripheral decarboxylase inhibitor was found to reduce the serum prolactin concentration. This combination seemed to behave like a DA agonist. This effect is not produced by the decarboxylase inhibitor per se. A possible explanation is that 5-HTP is converted to 5-HT in CA-ergic neurons, that 5-HT supersedes the CA from the stores, and that some of the CA reach the synaptic cleft and stimulate CA receptors. Another possible explanation is that 5-HTP decarboxylase is centrally inhibited as well, and that an effect of 5-HTP itself is involved here. In view of the observations made it is doubtful whether the therapeutic effect of 5-HTP combined with a peripheral decarboxylase inhibitor in depressions and myoclonus can in fact be atributed to activation of central serotonergic systems."}
{"id": "PMID:1088303", "title": "[Study on tumor affinity of technetium-99m-labeled radiopharmaceuticals. (1) 99mTc-pertechnetate, 99mTc-human serum albumin, and 99mTc-trasylol].", "content": "This paper describes biologic distributions, sequential images and macroautoradiograms of 99mTcO4 (pertechnetate), 99mTc-Sn-HSA (human serum albumin) and 99mTc-Sn-TSL (trasylol) in tumor-bearing mice as the first report on tumor affinity of 99mTc-labeled radiopharmaceutical. (1) Maximum tumor concentration (% administered dose/g of tissue weight) of 99mTcO4, 99mTc-HSA and 99mTc-TSL in Ehrlich's tumor-bearing mice resulted in 2.03+/-0.57 at 1 hr, 4.02+/-0.19 at 3 hr and 1.97+/-0.31 at 1 hr respectively. (2) However, tumor to blood concentration ratio of 99mTc-HSA was lowest among them. (3) The corrected tumor accumulation (% 100g dose/g of tissue wt.=% dose/% body weight) of 99mTc-TSL to Ehrlich's tumor in mouse was not different from that of Yoshida's sarcoma in rat, on the contrary to our expectation that the tumor concentration of 99mTc-TSL in them might be different due to differency of the tissue fibrinolytic activity between the respective tumors. (4) Sequential images of the implanted tumor in mouse was best positively delineated with 99mTc-HSA. (5) Macroautoradiograms of Ehrlich's tumor with 99mTcO4, 99mTc-HSA and 99mTc-TSL demonstrated the following findings: all of them were not only accumulated markedly into the tumor cells which were shown as basophilic tissue with H\u00e4matoxylin-Eosin staining but also accumulated around the tumor tissue and on the interstitial tissue which were stained as eosinophilic tissue with the above same staining.", "contents": "[Study on tumor affinity of technetium-99m-labeled radiopharmaceuticals. (1) 99mTc-pertechnetate, 99mTc-human serum albumin, and 99mTc-trasylol]. This paper describes biologic distributions, sequential images and macroautoradiograms of 99mTcO4 (pertechnetate), 99mTc-Sn-HSA (human serum albumin) and 99mTc-Sn-TSL (trasylol) in tumor-bearing mice as the first report on tumor affinity of 99mTc-labeled radiopharmaceutical. (1) Maximum tumor concentration (% administered dose/g of tissue weight) of 99mTcO4, 99mTc-HSA and 99mTc-TSL in Ehrlich's tumor-bearing mice resulted in 2.03+/-0.57 at 1 hr, 4.02+/-0.19 at 3 hr and 1.97+/-0.31 at 1 hr respectively. (2) However, tumor to blood concentration ratio of 99mTc-HSA was lowest among them. (3) The corrected tumor accumulation (% 100g dose/g of tissue wt.=% dose/% body weight) of 99mTc-TSL to Ehrlich's tumor in mouse was not different from that of Yoshida's sarcoma in rat, on the contrary to our expectation that the tumor concentration of 99mTc-TSL in them might be different due to differency of the tissue fibrinolytic activity between the respective tumors. (4) Sequential images of the implanted tumor in mouse was best positively delineated with 99mTc-HSA. (5) Macroautoradiograms of Ehrlich's tumor with 99mTcO4, 99mTc-HSA and 99mTc-TSL demonstrated the following findings: all of them were not only accumulated markedly into the tumor cells which were shown as basophilic tissue with H\u00e4matoxylin-Eosin staining but also accumulated around the tumor tissue and on the interstitial tissue which were stained as eosinophilic tissue with the above same staining."}
{"id": "PMID:1088317", "title": "Antigen perception by T lymphocytes. Influence of the major histocompatibility complex.", "content": "There is considerable evidence that T cell activation to soluble antigens occurs only if this is processed by macrophages and displayed appropriately on the plasma membrane in association with products of the genes of the major histocompatibility complex. The genes responsible differ according to the antigens involved. For cytotoxicity, targets and killer T cells must share K or D region gene products. For delayed type hypersensitivity to fowl gammaglobulin in mice, I-A identity is necessary; for dinitrofluorobenzene, identity at either the I, K or D region is sufficient. Experiments using three different approaches do not support the notion that these genetic constraints are due to the necessity for the T cell and stimulator cell to match an identical gene product or \"cell interaction molecule.\" Rather they favour the hypothesis that activated T cells recognize antigen and products of genes of the major histocompatibility complex. The implications of the results are discussed in terms of the mode of action of immune responsiveness (Ir) genes and of the possible parallel evolution of T cell receptors for antigen and gene products of the major histocompatibility complex.", "contents": "Antigen perception by T lymphocytes. Influence of the major histocompatibility complex. There is considerable evidence that T cell activation to soluble antigens occurs only if this is processed by macrophages and displayed appropriately on the plasma membrane in association with products of the genes of the major histocompatibility complex. The genes responsible differ according to the antigens involved. For cytotoxicity, targets and killer T cells must share K or D region gene products. For delayed type hypersensitivity to fowl gammaglobulin in mice, I-A identity is necessary; for dinitrofluorobenzene, identity at either the I, K or D region is sufficient. Experiments using three different approaches do not support the notion that these genetic constraints are due to the necessity for the T cell and stimulator cell to match an identical gene product or \"cell interaction molecule.\" Rather they favour the hypothesis that activated T cells recognize antigen and products of genes of the major histocompatibility complex. The implications of the results are discussed in terms of the mode of action of immune responsiveness (Ir) genes and of the possible parallel evolution of T cell receptors for antigen and gene products of the major histocompatibility complex."}
{"id": "PMID:1088331", "title": "Dependence of the heat production of active muscle on the length of muscle.", "content": "The dependence of the heat production of muscle on length was examined in isometric (with stretch) and isotonic (free of stretch) tetanuses of 2 s. The isometric heat production was significantly greater than the isotonic one at any length of muscle. The isometric heat coefficient (Flo/Q, where F is the active tension development of muscle measured in ponds, lo is the resting length measured in cm, Q is the heat production during the contraction at a given length measured in mcal) was not constant but dependent on the length of muscle, and had an extreme value near the resting length (lo). A close positive correlation was found between the active tension development and the heat production of the muscle.", "contents": "Dependence of the heat production of active muscle on the length of muscle. The dependence of the heat production of muscle on length was examined in isometric (with stretch) and isotonic (free of stretch) tetanuses of 2 s. The isometric heat production was significantly greater than the isotonic one at any length of muscle. The isometric heat coefficient (Flo/Q, where F is the active tension development of muscle measured in ponds, lo is the resting length measured in cm, Q is the heat production during the contraction at a given length measured in mcal) was not constant but dependent on the length of muscle, and had an extreme value near the resting length (lo). A close positive correlation was found between the active tension development and the heat production of the muscle."}
{"id": "PMID:1088332", "title": "The effect of anti-lymphocyte and anti-thymocyte serum on the pathogenesis of Rauscher leukaemia in inbred mouse strains.", "content": "Rauscher virus causes in inbred Balb/c mice a rapidly progressing hyperacute, in C57B1/10Sn mice an incipient, spontaneously healing leukaemia. In DBA/1 and DBA/2 mice the appearance of leukaemia is followed by a partial remissions then by an exacerbation of the disease. The infection in C57B1/10Sn, DBA/1 and DBA/2 mice results in a significant tumour-specific immune response. Inhibition of the immune response is followed by an increased progression of leukaemia in DBA/1 and DBA/2 mice only. It is assumed that in C57B1/10Sn mice the remission of incipient leukaemia is associated with a resistance determined in the target cells, whereas in DBA/1 and DBA/2 mice the remission is due to a tumour-specific immune response. As in animals treated with anti-lymphocyte and anti-thymocyte serum the course of the disease runs proportionally to the degree of the inhibition of the immune response, the tumour-specific antibodies play a decisive role in the elimination of the tumour cells. In Balb/c, DBA/1 and DBA/2 mouse strains failing to exhibit a spontaneous reversion of the tumour cells, the appearance of a significant tumour-specific immune response depends on the resistance against the helper component of the Rauscher virus complex.", "contents": "The effect of anti-lymphocyte and anti-thymocyte serum on the pathogenesis of Rauscher leukaemia in inbred mouse strains. Rauscher virus causes in inbred Balb/c mice a rapidly progressing hyperacute, in C57B1/10Sn mice an incipient, spontaneously healing leukaemia. In DBA/1 and DBA/2 mice the appearance of leukaemia is followed by a partial remissions then by an exacerbation of the disease. The infection in C57B1/10Sn, DBA/1 and DBA/2 mice results in a significant tumour-specific immune response. Inhibition of the immune response is followed by an increased progression of leukaemia in DBA/1 and DBA/2 mice only. It is assumed that in C57B1/10Sn mice the remission of incipient leukaemia is associated with a resistance determined in the target cells, whereas in DBA/1 and DBA/2 mice the remission is due to a tumour-specific immune response. As in animals treated with anti-lymphocyte and anti-thymocyte serum the course of the disease runs proportionally to the degree of the inhibition of the immune response, the tumour-specific antibodies play a decisive role in the elimination of the tumour cells. In Balb/c, DBA/1 and DBA/2 mouse strains failing to exhibit a spontaneous reversion of the tumour cells, the appearance of a significant tumour-specific immune response depends on the resistance against the helper component of the Rauscher virus complex."}
{"id": "PMID:1088326", "title": "[Role of salivary kallikrein in the pathogenesis of periodontosis].", "content": "In patients with parodontosis the distinct increase in salivary kallikrein secretion was found. In Wistar rats the intensity of age atrophy of alveoli dentalis correlated with BAEE-esterase activity of mixed saliva. Desalivation considerably decreased the rate of age atrophy of parodontosis in rats. Salivary kallikrein, penetrating in active form into parodontal tissues, caused the pronounced vasodilation in dogs and golden hamsters. By means of kallikrein preparations an intensive inflammatory-dystrophical process was reproduced in parodontal tissues of Wistar rats. The local treatment of patients with parodontosis by kallikrein inhibitors was found to be especially effective.", "contents": "[Role of salivary kallikrein in the pathogenesis of periodontosis]. In patients with parodontosis the distinct increase in salivary kallikrein secretion was found. In Wistar rats the intensity of age atrophy of alveoli dentalis correlated with BAEE-esterase activity of mixed saliva. Desalivation considerably decreased the rate of age atrophy of parodontosis in rats. Salivary kallikrein, penetrating in active form into parodontal tissues, caused the pronounced vasodilation in dogs and golden hamsters. By means of kallikrein preparations an intensive inflammatory-dystrophical process was reproduced in parodontal tissues of Wistar rats. The local treatment of patients with parodontosis by kallikrein inhibitors was found to be especially effective."}
{"id": "PMID:1088343", "title": "The rate of initiation of treatment for hypertension in a community, 1971--1975.", "content": "In 1971 persons aged 50-59 in a community were screened for hypertension and those with diastolic BP greater than or equal to 110 mmHg were notified. On reviewing the sample in 1975 the treatment rate had doubled to 22%. Screening in 1971 was followed by an immediate rise in numbers starting treatment, most being notified cases. Thereafter initiation of treatment at a rate of 3-8 cases per 100 persons per year chiefly reflected identification of subjects deemed to need treatment in the ordinary course of medical practice. Of those starting treatment during the four years 25% had stopped it by 1975. The influence of screening and other factors on the rate of initiation of treatment and adherence to it are discussed. With an already high attendance rate at doctors, regular screening of this whole age group seems feasible within the framework of general practice. The high treatment rate found in 1975 underlines the urgent need for the indications currently used for starting treatment to be validated.", "contents": "The rate of initiation of treatment for hypertension in a community, 1971--1975. In 1971 persons aged 50-59 in a community were screened for hypertension and those with diastolic BP greater than or equal to 110 mmHg were notified. On reviewing the sample in 1975 the treatment rate had doubled to 22%. Screening in 1971 was followed by an immediate rise in numbers starting treatment, most being notified cases. Thereafter initiation of treatment at a rate of 3-8 cases per 100 persons per year chiefly reflected identification of subjects deemed to need treatment in the ordinary course of medical practice. Of those starting treatment during the four years 25% had stopped it by 1975. The influence of screening and other factors on the rate of initiation of treatment and adherence to it are discussed. With an already high attendance rate at doctors, regular screening of this whole age group seems feasible within the framework of general practice. The high treatment rate found in 1975 underlines the urgent need for the indications currently used for starting treatment to be validated."}
{"id": "PMID:1088344", "title": "Thymus with B lymphocytes in chronic lymphocytic leukaemia.", "content": "A 67-year-old man with a diagnosis of chronic lymphocytic leukaemia died suddenly following a coronary occlusion. Peripheral blood lymphocytes labelled with fluorescein conjugated polyvalent antisera to immunoglobulin and immunoglobulin G, and failed to label with absorbed antithymocyte serum. At necropsy, a large thymic tumour, and enlarged mediastinal lymph nodes also contained immunoglobulin G bearing B lymphocytes. The paradoxical finding of B lymphocytes with monoclonal surface immunoglobulin in a thymic mass is most unusual in chronic lymphocytic leukaemia.", "contents": "Thymus with B lymphocytes in chronic lymphocytic leukaemia. A 67-year-old man with a diagnosis of chronic lymphocytic leukaemia died suddenly following a coronary occlusion. Peripheral blood lymphocytes labelled with fluorescein conjugated polyvalent antisera to immunoglobulin and immunoglobulin G, and failed to label with absorbed antithymocyte serum. At necropsy, a large thymic tumour, and enlarged mediastinal lymph nodes also contained immunoglobulin G bearing B lymphocytes. The paradoxical finding of B lymphocytes with monoclonal surface immunoglobulin in a thymic mass is most unusual in chronic lymphocytic leukaemia."}
{"id": "PMID:1088345", "title": "Immunological changes and liver disease associated with alpha1-antitrypsin deficiency.", "content": "A female aged 60 years with heterozygous alpha-1-antitrypsin deficiency developed a progressive and ultimately fatal liver disease with the clinical, biochemical, immunological and histological characteristics of active chronic hepatitis. It is suggested that the hepatic disease of A-AT deficiency be included among the types of liver disease which may initiate a progressive immunopathic response.", "contents": "Immunological changes and liver disease associated with alpha1-antitrypsin deficiency. A female aged 60 years with heterozygous alpha-1-antitrypsin deficiency developed a progressive and ultimately fatal liver disease with the clinical, biochemical, immunological and histological characteristics of active chronic hepatitis. It is suggested that the hepatic disease of A-AT deficiency be included among the types of liver disease which may initiate a progressive immunopathic response."}
{"id": "PMID:1088339", "title": "[Histiocytosis management (author's transl)].", "content": "Seven cases of Histiocytosis have been treated with VB and PR. Five of them that belong to grup C (according to Oberman's classification) experimented favorable resuts. Likewise, we have been able to prove that prognosis of these children depend on: their age at the onset of the disease; the original site of the disease; the extension of the lesions and the maintenance of prolonged therapy.", "contents": "[Histiocytosis management (author's transl)]. Seven cases of Histiocytosis have been treated with VB and PR. Five of them that belong to grup C (according to Oberman's classification) experimented favorable resuts. Likewise, we have been able to prove that prognosis of these children depend on: their age at the onset of the disease; the original site of the disease; the extension of the lesions and the maintenance of prolonged therapy."}
{"id": "PMID:1088347", "title": "[Study of the heterogenetic antigens in vaccinal preparations of V. cholerae].", "content": "There proved to be a serological similarity between the antigens of the human small intestine, the stomach and the liver, and the antigens of various cholera vibrio fractions. No antigenic similarity was revealed in examination of the heart and kidney. Heterogenous antigen was found not only in the somatic V. cholerae antigen, strain 569 (B), but also in the cholerogen-toxoid obtained from it. At present it is the most widespread prophylactic preparation.", "contents": "[Study of the heterogenetic antigens in vaccinal preparations of V. cholerae]. There proved to be a serological similarity between the antigens of the human small intestine, the stomach and the liver, and the antigens of various cholera vibrio fractions. No antigenic similarity was revealed in examination of the heart and kidney. Heterogenous antigen was found not only in the somatic V. cholerae antigen, strain 569 (B), but also in the cholerogen-toxoid obtained from it. At present it is the most widespread prophylactic preparation."}
{"id": "PMID:1088350", "title": "Impact of control measures on malaria transmission and general mortality.", "content": "This paper is an attempt to relate modifications observed in general and infant mortality rates with the dynamic changes in transmission induced by malaria control measures. The observations indicated relationships between the efficacy of control and a decrease in mortality. The daily parasitological inoculation rate was reduced from 0.00958 infective bites per individual before treatment to 0.00037 after treatment (a decrease of 96%). In two years, general mortality decreased from 23.9 to 13.5 deaths per 1000 population and infant mortality decreased from 157 to 93 per 1000 live births. This indirect benefit of malaria control deserves attention in a wider assessment of measures directed against vector-borne diseases.", "contents": "Impact of control measures on malaria transmission and general mortality. This paper is an attempt to relate modifications observed in general and infant mortality rates with the dynamic changes in transmission induced by malaria control measures. The observations indicated relationships between the efficacy of control and a decrease in mortality. The daily parasitological inoculation rate was reduced from 0.00958 infective bites per individual before treatment to 0.00037 after treatment (a decrease of 96%). In two years, general mortality decreased from 23.9 to 13.5 deaths per 1000 population and infant mortality decreased from 157 to 93 per 1000 live births. This indirect benefit of malaria control deserves attention in a wider assessment of measures directed against vector-borne diseases."}
{"id": "PMID:1088351", "title": "The impact of propoxur on Anopheles gambiae s.1. and some other anopheline populations, and its relationship with some pre-spraying variables.", "content": "A significant and stable difference in the residual night-biting collection (NBC, observed/expected) of A. gambiae s.l. was observed among different villages of the West African savannah when sprayed for 2 years with propoxur. The residual mosquito density of a given village was positively associated with some of its pre-spraying characteristics: the NBC/PSC (pyrethrum spray collections) ratio, the median biting hour, and possibly the proportion of species B. It was not significantly associated with several other pre-spraying characteristics (absolute mosquito density, the ratios between NBC indoors and NBC outdoors, between ETC (exit-trap collections) and PSC, between fed and gravid in the PSC or ETC, between males and females in the PSC), and was not associated with variations in recorded coverage, with latitude, or with distance from unsprayed villages.In a comparison between A. gambiae s.l., A. funestus, and A. pharoensis, the residual NBC (observed/expected) was positively associated with the pre-spraying NBC/PSC ratio, and was negatively associated with the median biting hour.The pre-spraying NBC/PSC ratio thus appears to be a predictor of the variation, between villages or species, in the reduction of the NBC by residual spraying, and may assist in forecasting the effect of a residual insecticide. The limitations of any absolute prediction must, however, be kept in mind.", "contents": "The impact of propoxur on Anopheles gambiae s.1. and some other anopheline populations, and its relationship with some pre-spraying variables. A significant and stable difference in the residual night-biting collection (NBC, observed/expected) of A. gambiae s.l. was observed among different villages of the West African savannah when sprayed for 2 years with propoxur. The residual mosquito density of a given village was positively associated with some of its pre-spraying characteristics: the NBC/PSC (pyrethrum spray collections) ratio, the median biting hour, and possibly the proportion of species B. It was not significantly associated with several other pre-spraying characteristics (absolute mosquito density, the ratios between NBC indoors and NBC outdoors, between ETC (exit-trap collections) and PSC, between fed and gravid in the PSC or ETC, between males and females in the PSC), and was not associated with variations in recorded coverage, with latitude, or with distance from unsprayed villages.In a comparison between A. gambiae s.l., A. funestus, and A. pharoensis, the residual NBC (observed/expected) was positively associated with the pre-spraying NBC/PSC ratio, and was negatively associated with the median biting hour.The pre-spraying NBC/PSC ratio thus appears to be a predictor of the variation, between villages or species, in the reduction of the NBC by residual spraying, and may assist in forecasting the effect of a residual insecticide. The limitations of any absolute prediction must, however, be kept in mind."}
{"id": "PMID:1088352", "title": "Aircraft disinfection: the physical and insecticidal characteristics of (+)-phenothrin applied by aerosol at \"blocks away\".", "content": "A 2% formulation of (+)-phenothrin dispensed from 340-g or break-off tip aerosol cans was highly effective against mosquitos in aircraft disinsection trials. Two minutes after application at \"blocks away\", the mass median diameter of the dispersed drops was 5.1-6.2 mum. The concentration of the insecticide in the cabin fell to 10% of the original concentration after 9.2 min. No odour or irritation was caused by the use of this insecticide.", "contents": "Aircraft disinfection: the physical and insecticidal characteristics of (+)-phenothrin applied by aerosol at \"blocks away\". A 2% formulation of (+)-phenothrin dispensed from 340-g or break-off tip aerosol cans was highly effective against mosquitos in aircraft disinsection trials. Two minutes after application at \"blocks away\", the mass median diameter of the dispersed drops was 5.1-6.2 mum. The concentration of the insecticide in the cabin fell to 10% of the original concentration after 9.2 min. No odour or irritation was caused by the use of this insecticide."}
{"id": "PMID:1088354", "title": "Techniques for estimating densities of Bulinus truncatus rohlfsi and its horizontal distribution in Volta Lake, Ghana.", "content": "Bulinus truncatus rohlfsi is the intermediate host of urinary schistosomiasis, which is highly endemic in the man-made Volta Lake. In 1971, a WHO/UNDP schistosomiasis project was established in the Pawmpawm branch and part of the Afram branch of Volta Lake. Malacological findings of the preliminary phase indicated that the snails were distributed in the littoral zone of the lake, and that this distribution was correlated with the presence of vegetation, especially Ceratophyllum. Transmission nearly always occurred in \"water contact sites\", i.e., places where people come into contact with water.A snail-sampling technique with palm-leaf mats was developed and standardized after it had been shown in sensitivity trials to compare favourably with a modified version of the \"man-time\" sampling method, in which the number of snails collected per man-hour is recorded. It is recommended that both these methods should be used to conduct ecological studies of B. rohlfsi in water contact sites.", "contents": "Techniques for estimating densities of Bulinus truncatus rohlfsi and its horizontal distribution in Volta Lake, Ghana. Bulinus truncatus rohlfsi is the intermediate host of urinary schistosomiasis, which is highly endemic in the man-made Volta Lake. In 1971, a WHO/UNDP schistosomiasis project was established in the Pawmpawm branch and part of the Afram branch of Volta Lake. Malacological findings of the preliminary phase indicated that the snails were distributed in the littoral zone of the lake, and that this distribution was correlated with the presence of vegetation, especially Ceratophyllum. Transmission nearly always occurred in \"water contact sites\", i.e., places where people come into contact with water.A snail-sampling technique with palm-leaf mats was developed and standardized after it had been shown in sensitivity trials to compare favourably with a modified version of the \"man-time\" sampling method, in which the number of snails collected per man-hour is recorded. It is recommended that both these methods should be used to conduct ecological studies of B. rohlfsi in water contact sites."}
{"id": "PMID:1088355", "title": "Effects of environmental factors on the molluscicidal activities of slow-release hexabutyldistannoxane and copper sulfate.", "content": "Laboratory experiments were conducted to study the molluscicidal activities of slow-release hexabutyldistannoxane (TBTO) and copper sulfate under various environmental conditions. Organic materials such as mud and weeds reduced the molluscicidal efficacy of both chemicals. TBTO can be considered a good long-lasting molluscicide but, because of uncertainty as to its general toxic effects, it should not be used in field trials. The molluscicidal activity of slow-release copper sulfate was short-lived in plain lake water and was nil in the presence of mud or weeds at the concentration used.", "contents": "Effects of environmental factors on the molluscicidal activities of slow-release hexabutyldistannoxane and copper sulfate. Laboratory experiments were conducted to study the molluscicidal activities of slow-release hexabutyldistannoxane (TBTO) and copper sulfate under various environmental conditions. Organic materials such as mud and weeds reduced the molluscicidal efficacy of both chemicals. TBTO can be considered a good long-lasting molluscicide but, because of uncertainty as to its general toxic effects, it should not be used in field trials. The molluscicidal activity of slow-release copper sulfate was short-lived in plain lake water and was nil in the presence of mud or weeds at the concentration used."}
{"id": "PMID:1088356", "title": "Snail control in urban sites in Brazil with slow-release hexabutyldistannoxane and pentachlorophenol.", "content": "Slow release formulations of hexabutyldistannoxane (TBTO) and pentachlorophenol (PCP) were tested for the control of Biomphalaria tenagophila in 52 urban sites in Rio de Janeiro. TBTO acted faster and lasted longer than PCP and at 15 g/m(2) it eliminated snails from 76% of the treated sites for 1 year. Water pollution and rate of flow had no significant influence on the molluscicidal properties of either compound, but alkalinity lowered the activity of TBTO. Failure to control snail populations was due mainly to human interference and to the non-treatment of adjacent breeding sites that were temporarily dry and therefore overlooked.", "contents": "Snail control in urban sites in Brazil with slow-release hexabutyldistannoxane and pentachlorophenol. Slow release formulations of hexabutyldistannoxane (TBTO) and pentachlorophenol (PCP) were tested for the control of Biomphalaria tenagophila in 52 urban sites in Rio de Janeiro. TBTO acted faster and lasted longer than PCP and at 15 g/m(2) it eliminated snails from 76% of the treated sites for 1 year. Water pollution and rate of flow had no significant influence on the molluscicidal properties of either compound, but alkalinity lowered the activity of TBTO. Failure to control snail populations was due mainly to human interference and to the non-treatment of adjacent breeding sites that were temporarily dry and therefore overlooked."}
{"id": "PMID:1088357", "title": "Assay of togavirus haemagglutination-inhibition antibodies by the micro method: loss of information and its rectification.", "content": "Haemagglutination inhibition (HI) activity was assayed by the micro and macro methods in immune sera prepared against four togaviruses. HI titres were always 4 to 8 times lower by the micro method, and coincided with 4 to 8 times lower haemagglutinin titres in micro method assays. Because of this phenomenon, positive sera with HI macro method titres lower than 1: 80 will be false negative for HI by the micro method when tests begin at a 1: 10 serum dilution. This was confirmed for a number of human sera tested for West Nile virus HI activity. As a consequence of the difference between titres given by the two assay methods, results of seroepidemiological studies may be distorted. Use of a system that combines both the macro and micro methods could rectify this distortion.", "contents": "Assay of togavirus haemagglutination-inhibition antibodies by the micro method: loss of information and its rectification. Haemagglutination inhibition (HI) activity was assayed by the micro and macro methods in immune sera prepared against four togaviruses. HI titres were always 4 to 8 times lower by the micro method, and coincided with 4 to 8 times lower haemagglutinin titres in micro method assays. Because of this phenomenon, positive sera with HI macro method titres lower than 1: 80 will be false negative for HI by the micro method when tests begin at a 1: 10 serum dilution. This was confirmed for a number of human sera tested for West Nile virus HI activity. As a consequence of the difference between titres given by the two assay methods, results of seroepidemiological studies may be distorted. Use of a system that combines both the macro and micro methods could rectify this distortion."}
{"id": "PMID:1088358", "title": "Characteristics of the spread of a wildlife rabies epidemic in Europe.", "content": "The control of rabies in wildlife by reducing the fox population has led in Europe to inconsistent results, since little was known of the dynamics of the fox population and the interaction between rabies epidemics, host populations, and control measures. As part of the WHO/FAO Coordinated Research Programme on Wildlife Rabies in Europe, data on epidemics and persisting reservoirs of the disease were processed by computer. The results led to a better understanding of the mechanism of spread of the epidemic and to proposals for the improvement of rabies control in animals and the protection of man.", "contents": "Characteristics of the spread of a wildlife rabies epidemic in Europe. The control of rabies in wildlife by reducing the fox population has led in Europe to inconsistent results, since little was known of the dynamics of the fox population and the interaction between rabies epidemics, host populations, and control measures. As part of the WHO/FAO Coordinated Research Programme on Wildlife Rabies in Europe, data on epidemics and persisting reservoirs of the disease were processed by computer. The results led to a better understanding of the mechanism of spread of the epidemic and to proposals for the improvement of rabies control in animals and the protection of man."}
{"id": "PMID:1088362", "title": "Gastric metastasis from breast carcinoma: emergency snare-ectomy for control of hemorrhage.", "content": "In a 65-year-old woman, radiological and endoscopical findings of gastric metastasis from breast carcinoma are described. Significant upper gastrointestinal bleeding necessitated an emergency gastroscopy. The ulcerated nodular lesion was bleeding and removed by diathermy loop via fiberoptic gastroscopy.", "contents": "Gastric metastasis from breast carcinoma: emergency snare-ectomy for control of hemorrhage. In a 65-year-old woman, radiological and endoscopical findings of gastric metastasis from breast carcinoma are described. Significant upper gastrointestinal bleeding necessitated an emergency gastroscopy. The ulcerated nodular lesion was bleeding and removed by diathermy loop via fiberoptic gastroscopy."}
{"id": "PMID:1088363", "title": "The Mallory-Weiss-syndrome: a clinical study of 20 cases.", "content": "This is an account of 20 cases of MWS, representing 8% of a series of 247 consecutive upper GI-bleedings. Men above 40 years and persons with severe concomitant disease are specially at risk. The prognosis is rather good, mortality from bleeding being 10%. Surgery should not be undertaken unless all conservative measures, including vasopressin infusion and even Sengstaken-Blakemore tube have been applied. The typical history, a hiatal hernia and alcohol intake are frequently missing. Neither studies of gastric acid secretion nor esophageal manometry disclosed new pathogenic aspects.", "contents": "The Mallory-Weiss-syndrome: a clinical study of 20 cases. This is an account of 20 cases of MWS, representing 8% of a series of 247 consecutive upper GI-bleedings. Men above 40 years and persons with severe concomitant disease are specially at risk. The prognosis is rather good, mortality from bleeding being 10%. Surgery should not be undertaken unless all conservative measures, including vasopressin infusion and even Sengstaken-Blakemore tube have been applied. The typical history, a hiatal hernia and alcohol intake are frequently missing. Neither studies of gastric acid secretion nor esophageal manometry disclosed new pathogenic aspects."}
{"id": "PMID:1088366", "title": "Psyche and rheuma.", "content": "The paper is based on discussions which took place in a seminar at the European League against Rheumatism Meeting in Helsinki in 1975. The importance of assessing the emotional response in rheumatoid disease, as well as the disease process, is stressed. It is important to consider the effect of the discovery by the patient that severe disease is present. Considerable support by general practitioners and consultants may be necessary at this time. There has been much discussion as to whether patients suffering from rheumatoid arthritis have a different type of personality than other subjects. The evidence is not convincing. The role of stress in the aetiology of rheumatoid arthritis is not properly understood. Anxiolytic and antidepressant drugs may help patients to overcome the anxiety and depression associated with rheumatoid arthritis. Children suffering from the disease present a special problem. The incapacity and isolation, especially in old age, which osteo-arthritis brings may lead to depression and antidepressant therapy is often required. When rheumatic pain seems out of proportion to the physical findings, psychogenic rheumatism should be considered. Rheumatic pains occur in masked depression and low backache may be a depressive equivalent. Pain may be used by some patients to communicate distress.", "contents": "Psyche and rheuma. The paper is based on discussions which took place in a seminar at the European League against Rheumatism Meeting in Helsinki in 1975. The importance of assessing the emotional response in rheumatoid disease, as well as the disease process, is stressed. It is important to consider the effect of the discovery by the patient that severe disease is present. Considerable support by general practitioners and consultants may be necessary at this time. There has been much discussion as to whether patients suffering from rheumatoid arthritis have a different type of personality than other subjects. The evidence is not convincing. The role of stress in the aetiology of rheumatoid arthritis is not properly understood. Anxiolytic and antidepressant drugs may help patients to overcome the anxiety and depression associated with rheumatoid arthritis. Children suffering from the disease present a special problem. The incapacity and isolation, especially in old age, which osteo-arthritis brings may lead to depression and antidepressant therapy is often required. When rheumatic pain seems out of proportion to the physical findings, psychogenic rheumatism should be considered. Rheumatic pains occur in masked depression and low backache may be a depressive equivalent. Pain may be used by some patients to communicate distress."}
{"id": "PMID:1088368", "title": "Electrical stimulation in multiple sclerosis.", "content": "When electrodes were implanted in the spinal cord of a patient with MS--for dorsal column stimulation to relieve intractable pain--she regained considerable movement in her legs. Subsequently such stimulation has been employed in more than 70 other patients, and many have regained voluntary control over their arms, legs, and sphincters. The procedure is detailed and its implications are explored.", "contents": "Electrical stimulation in multiple sclerosis. When electrodes were implanted in the spinal cord of a patient with MS--for dorsal column stimulation to relieve intractable pain--she regained considerable movement in her legs. Subsequently such stimulation has been employed in more than 70 other patients, and many have regained voluntary control over their arms, legs, and sphincters. The procedure is detailed and its implications are explored."}
{"id": "PMID:1088364", "title": "[Effect of lithium on the central serotonin- and adrenergic processes after its chronic administration].", "content": "With its chronic administration in a dose of 100 mg/kg lithium carbonate inhibited shaking of the head induced in mice with 5-hydroxytryptophan (5-HTP). This effect did not differ from the action following a single injection of lithium, when the interval between injection of lithium and of 5-HTP was one hour. With the interval lengthened to 24 hours the frequency of shaking diminished only under the effect of chronic administration. At the 5th, 10th and 21st day of a daily administration lithium failed to produce any effect on the hypothermal action of a reserpine-like agent Po 4-1284, but would reduce the protective action of imipramine in a ptosis test. A single injection of lithium made against the background of a chronic injection of water produced an opposite effect, viz. it significantly reduced the protective action of imipramine in hypothermia, but did not affect it with reference to ptosis. Hence, chronic administration of lithium leads to potentiation in its action of the serotonin-negative and central adreno-negative componets and to extenuating the peripheral adreno-negative component.", "contents": "[Effect of lithium on the central serotonin- and adrenergic processes after its chronic administration]. With its chronic administration in a dose of 100 mg/kg lithium carbonate inhibited shaking of the head induced in mice with 5-hydroxytryptophan (5-HTP). This effect did not differ from the action following a single injection of lithium, when the interval between injection of lithium and of 5-HTP was one hour. With the interval lengthened to 24 hours the frequency of shaking diminished only under the effect of chronic administration. At the 5th, 10th and 21st day of a daily administration lithium failed to produce any effect on the hypothermal action of a reserpine-like agent Po 4-1284, but would reduce the protective action of imipramine in a ptosis test. A single injection of lithium made against the background of a chronic injection of water produced an opposite effect, viz. it significantly reduced the protective action of imipramine in hypothermia, but did not affect it with reference to ptosis. Hence, chronic administration of lithium leads to potentiation in its action of the serotonin-negative and central adreno-negative componets and to extenuating the peripheral adreno-negative component."}
{"id": "PMID:1088365", "title": "[Toxicity of benzhydrylurea under experimental conditions].", "content": "Benzhydrylurea, which displays an anticonvulsant action and is of low acute toxicity in capable after a 3-month administration to rats to inhibit the growth of male-rats, intensify the excretion of protein with the urine, raise the blood sugar level (with its single administration--to reduce), provoke dystrophic changes in the liver, kidneys and central nervous system. Following a 1--3-week introduction of the substance guinea pigs and cats demonstrate hemorrhagic erosions in the stomach and small intestine (but not rabbits, dogs and rats), the appearance of pathological forms of erythrocytes, while guinea pigs suffer from leucopoietic disorders.", "contents": "[Toxicity of benzhydrylurea under experimental conditions]. Benzhydrylurea, which displays an anticonvulsant action and is of low acute toxicity in capable after a 3-month administration to rats to inhibit the growth of male-rats, intensify the excretion of protein with the urine, raise the blood sugar level (with its single administration--to reduce), provoke dystrophic changes in the liver, kidneys and central nervous system. Following a 1--3-week introduction of the substance guinea pigs and cats demonstrate hemorrhagic erosions in the stomach and small intestine (but not rabbits, dogs and rats), the appearance of pathological forms of erythrocytes, while guinea pigs suffer from leucopoietic disorders."}
{"id": "PMID:1088371", "title": "Cancer in a familial IgA deficiency patient: abnormal chromosomes and B lymphocytes.", "content": "Two primary cancers were found in a patient with a familial IgA deficiency. Although cytogenetic studies showed nonconsistent pseudodiploid chromosomal pattern in the peripheral blood lymphocyte cultures, there were two metaphases with a deleted short arm of an E (17-18) chromosome and several metaphases with an abnormally large chromosome, larger than chromosome number 1. Also, there were 37 B lymphocytes per mm3 in her peripheral blood which was much lower than other radiation-treated cancer patients. The absence of IgA, the decrease of peripheral B lymphocytes and the chromosomal abnormality might have played a role in her developing the cancers.", "contents": "Cancer in a familial IgA deficiency patient: abnormal chromosomes and B lymphocytes. Two primary cancers were found in a patient with a familial IgA deficiency. Although cytogenetic studies showed nonconsistent pseudodiploid chromosomal pattern in the peripheral blood lymphocyte cultures, there were two metaphases with a deleted short arm of an E (17-18) chromosome and several metaphases with an abnormally large chromosome, larger than chromosome number 1. Also, there were 37 B lymphocytes per mm3 in her peripheral blood which was much lower than other radiation-treated cancer patients. The absence of IgA, the decrease of peripheral B lymphocytes and the chromosomal abnormality might have played a role in her developing the cancers."}
{"id": "PMID:1088372", "title": "[Free radical processes in the embryogenesis of Anura].", "content": "The localization of free radical processes and changes in their level during the common frog development have been studied by means of grafted copolymerisation and autoradiography. The maps of distribution of relative concentrations of free radicals were obtained for the beginning of cleavage, blastula, gastrula and neurula. The distinct regionalization was found in the beginning of cleavage: the concentration of the free radicals in the cortical layer and dorsal half of embryo is lower than in the central area and ventral half, resp. At the early blastula stage this regionalization is preserved in its general features. The region of embryo characterized by active free radical processes corresponds to the presumptive endo- and mesoderm in the period of inductive interaction. The possible participation of regional changes in oxidative metabolism in the fertilized egg in the determination of cytoplasmic localization of morphogenetic potencies is discussed. At the later stages there were variations observed in free radicals concentration, which are discussed as being related to the determination and morphogenesis of some rudiments of embryo. A local rise of free radicals concentration was also found out in the eye rudiment just before the onset of its components differentiation.", "contents": "[Free radical processes in the embryogenesis of Anura]. The localization of free radical processes and changes in their level during the common frog development have been studied by means of grafted copolymerisation and autoradiography. The maps of distribution of relative concentrations of free radicals were obtained for the beginning of cleavage, blastula, gastrula and neurula. The distinct regionalization was found in the beginning of cleavage: the concentration of the free radicals in the cortical layer and dorsal half of embryo is lower than in the central area and ventral half, resp. At the early blastula stage this regionalization is preserved in its general features. The region of embryo characterized by active free radical processes corresponds to the presumptive endo- and mesoderm in the period of inductive interaction. The possible participation of regional changes in oxidative metabolism in the fertilized egg in the determination of cytoplasmic localization of morphogenetic potencies is discussed. At the later stages there were variations observed in free radicals concentration, which are discussed as being related to the determination and morphogenesis of some rudiments of embryo. A local rise of free radicals concentration was also found out in the eye rudiment just before the onset of its components differentiation."}
{"id": "PMID:1088373", "title": "[Study of development of ciliary folds and Zinn's zonules in Rana temporaria L. by means of scanning electron microscopy].", "content": "The development of ciliary folds and zonul of Zinn has been studied in the eyes of the common frog Rana temporaria L. by means of scanning electron microscopy. The development of ciliary folds begins at the stage 45 by the flexure of the external layer in the ciliary zone. At the stage 46 this process involves the internal layer and the folds become two-layered. The zonules of Zinn form before the folds of internal layer of the ciliary epithelium begin to form as separate bundles of fibers. At the stage 45 they are already distinct. Later the ciliary filaments fold in 2 felt-like layers -- zonula which pass from the equatorial lens zone and attach near orbiculum ciliaris. In the place of attachment the margin of zonul repeats the relief of folds, thus attaching to their whole surface, and individual filaments go farther, to orbiculum ciliaris. All these processes take place in they eye prior to the beginning of metamorphosis.", "contents": "[Study of development of ciliary folds and Zinn's zonules in Rana temporaria L. by means of scanning electron microscopy]. The development of ciliary folds and zonul of Zinn has been studied in the eyes of the common frog Rana temporaria L. by means of scanning electron microscopy. The development of ciliary folds begins at the stage 45 by the flexure of the external layer in the ciliary zone. At the stage 46 this process involves the internal layer and the folds become two-layered. The zonules of Zinn form before the folds of internal layer of the ciliary epithelium begin to form as separate bundles of fibers. At the stage 45 they are already distinct. Later the ciliary filaments fold in 2 felt-like layers -- zonula which pass from the equatorial lens zone and attach near orbiculum ciliaris. In the place of attachment the margin of zonul repeats the relief of folds, thus attaching to their whole surface, and individual filaments go farther, to orbiculum ciliaris. All these processes take place in they eye prior to the beginning of metamorphosis."}
{"id": "PMID:1088374", "title": "[Effect of various factors on the differentiation of hematopoietic stem cells].", "content": "The treatment in vitro of bone marrow cells in mice by phytohemagglutinin, concanavaline, or antilimphocytic globulin resulted in the suppression of exogenous hemopoietic colonies in the spleen of lethally irradiated (830r) syngenic recipients, whereas lipopolysaccharide, tuberculin, anti-theta serum or nati-gamma-globulin serum exerted no influence on the colony-forming function of hemopoietic stem cells. The morphological analysis of the ratio and cell composition of hemopoietic colonies has revealed no marked differences between the experimental and control groups. The suppression of hemopoietic stem cells by mitogens might be due both to their direct effect and indirect one, possibly, through a humoral factor.", "contents": "[Effect of various factors on the differentiation of hematopoietic stem cells]. The treatment in vitro of bone marrow cells in mice by phytohemagglutinin, concanavaline, or antilimphocytic globulin resulted in the suppression of exogenous hemopoietic colonies in the spleen of lethally irradiated (830r) syngenic recipients, whereas lipopolysaccharide, tuberculin, anti-theta serum or nati-gamma-globulin serum exerted no influence on the colony-forming function of hemopoietic stem cells. The morphological analysis of the ratio and cell composition of hemopoietic colonies has revealed no marked differences between the experimental and control groups. The suppression of hemopoietic stem cells by mitogens might be due both to their direct effect and indirect one, possibly, through a humoral factor."}
{"id": "PMID:1088379", "title": "Absence of Thy-1 antigen in L-cell X mouse lymphoma hybrids.", "content": "Hybrid clones arising after inactivated Sendai virus mediated cell fusion between a bromodeoxyuridine-resistant derivative of the BALB/c mouse lymphoma cell line, S49, and a thioguanine-resistant derivative (A9) of the C3H mouse fibroblast cell line, L929, were selected in HAT medium. The responses of the clones to growth inhibitors as well as their chromosome numbers were consistent with properties expected of hybrids. Hybrid clones expressed the major histocompatibility (H-2) surface antigens of both parental types, i.e., H-2d of BALB/c and H-2k of C3H. The Thy-1.2 antigen, expressed on the surface of the lymphoma parent but not the fibroblast parent, was not detected on the hybrids.", "contents": "Absence of Thy-1 antigen in L-cell X mouse lymphoma hybrids. Hybrid clones arising after inactivated Sendai virus mediated cell fusion between a bromodeoxyuridine-resistant derivative of the BALB/c mouse lymphoma cell line, S49, and a thioguanine-resistant derivative (A9) of the C3H mouse fibroblast cell line, L929, were selected in HAT medium. The responses of the clones to growth inhibitors as well as their chromosome numbers were consistent with properties expected of hybrids. Hybrid clones expressed the major histocompatibility (H-2) surface antigens of both parental types, i.e., H-2d of BALB/c and H-2k of C3H. The Thy-1.2 antigen, expressed on the surface of the lymphoma parent but not the fibroblast parent, was not detected on the hybrids."}
{"id": "PMID:1088386", "title": "Tumor virus effects on immunocyte precursor cells. Hemopoietic stem cell behavior and leukemogenic susceptibility.", "content": "Studies of Rauscher virus-induced erythroleukemia have demonstrated immunodepressive effects in the host and enhanced leukemogenesis with adjuvant administration. These observations led to the study of leukemic development in the NZB strain as a natural model of the experimentally adjuvant-stimulated animal. The results of such investigation would attribute the increased susceptibility of NZB mice to the possession of an enlarged population of pluripotent hemopoietic stem cells in active cell cycle. Studies with radiation chimeras have further shown that elevated endogenous spleen colony formation, the increased potential for autoimmunity, and for susceptibility to Rauscher viral leukemogenesis are all linked through the NZB hemopoietic system. It is concluded that the presence of an enlarged compartment of cyclically active stem cells may be an etiologic factor in the susceptibility to both virus-induced leukemia and the development of autoimmune disease.", "contents": "Tumor virus effects on immunocyte precursor cells. Hemopoietic stem cell behavior and leukemogenic susceptibility. Studies of Rauscher virus-induced erythroleukemia have demonstrated immunodepressive effects in the host and enhanced leukemogenesis with adjuvant administration. These observations led to the study of leukemic development in the NZB strain as a natural model of the experimentally adjuvant-stimulated animal. The results of such investigation would attribute the increased susceptibility of NZB mice to the possession of an enlarged population of pluripotent hemopoietic stem cells in active cell cycle. Studies with radiation chimeras have further shown that elevated endogenous spleen colony formation, the increased potential for autoimmunity, and for susceptibility to Rauscher viral leukemogenesis are all linked through the NZB hemopoietic system. It is concluded that the presence of an enlarged compartment of cyclically active stem cells may be an etiologic factor in the susceptibility to both virus-induced leukemia and the development of autoimmune disease."}
{"id": "PMID:1088387", "title": "Discussion paper: impairment of B-lymphocyte functions in concanavalin A-treated friend virus infected mice.", "content": "Friend leukemia virus (FLV) leukemogenesis was prevented by treatment of the virus with Concanavalin A (Con A). Mice infected with the lectin-treated virus, however, showed evidence of a dormant infection since infectious virus could be recovered for as long as 100 days. Humoral immune responses to sheep erythrocytes (SRBC), a thymus-dependent antigen, and to E. coli lipopolysaccharide (LPS), a thymus-independent antigen, were depressed (approximately 80-90%) in mice given the Con A-treated FLV. Cell transfer studies indicated that the impaired responsiveness to SRBC was related to a defect in B-lymphocyte function, similar to the impairment in mice infected with untreated FLV. The mitogenic response of splenocytes from Con A-FLV mice to E. coli LPS was also depressed as was the ability of Ig-bearing spleen cells to redistribute these immunoglobulin receptors into polar caps. The impaired immune responsiveness in the Con A-FLV infected mice appeared associated with the persistent virus infection and not to neoplastic transformation generally associated with leukemogenic process.", "contents": "Discussion paper: impairment of B-lymphocyte functions in concanavalin A-treated friend virus infected mice. Friend leukemia virus (FLV) leukemogenesis was prevented by treatment of the virus with Concanavalin A (Con A). Mice infected with the lectin-treated virus, however, showed evidence of a dormant infection since infectious virus could be recovered for as long as 100 days. Humoral immune responses to sheep erythrocytes (SRBC), a thymus-dependent antigen, and to E. coli lipopolysaccharide (LPS), a thymus-independent antigen, were depressed (approximately 80-90%) in mice given the Con A-treated FLV. Cell transfer studies indicated that the impaired responsiveness to SRBC was related to a defect in B-lymphocyte function, similar to the impairment in mice infected with untreated FLV. The mitogenic response of splenocytes from Con A-FLV mice to E. coli LPS was also depressed as was the ability of Ig-bearing spleen cells to redistribute these immunoglobulin receptors into polar caps. The impaired immune responsiveness in the Con A-FLV infected mice appeared associated with the persistent virus infection and not to neoplastic transformation generally associated with leukemogenic process."}
{"id": "PMID:1088391", "title": "Comparison of the shock-inducing effect of anti-mouse anti-thymocyte horse serum and normal horse serum. II. Tests with ten-day-old mice.", "content": "Studying the shock-inducing effect of ATS and NHS in young (10-day-old) mice weighing 10-11 g, the authors have found that normal serum proved to be a significantly stronger sensitizing agent. Such a difference in the shock-inducing effect of the two sera can no longer be observed in the course of challenge. The paper discusses the observed phenomenon, taking into account the data available on the ontogeny of the immune apparatus of the mice.", "contents": "Comparison of the shock-inducing effect of anti-mouse anti-thymocyte horse serum and normal horse serum. II. Tests with ten-day-old mice. Studying the shock-inducing effect of ATS and NHS in young (10-day-old) mice weighing 10-11 g, the authors have found that normal serum proved to be a significantly stronger sensitizing agent. Such a difference in the shock-inducing effect of the two sera can no longer be observed in the course of challenge. The paper discusses the observed phenomenon, taking into account the data available on the ontogeny of the immune apparatus of the mice."}
{"id": "PMID:1088390", "title": "Comparison of the shock-inducing effect of anti-mouse anti-thymocyte horse serum and normal horse serum. I. Experiments with three-week-old mice.", "content": "Sera administered intravenously to mice hypersensitized with normal horse serum and with horse serum of anti-lymphocytic activity, produce reactions that differ considerably in mice treated in different ways. The experiments of the authors indicate that the anaphylactogenic effect of ATS is substantially stronger than that or normal horse serum. The paper discusses the clinical and theoretical conclusions to be drawn from this phenomenon observed in the experiments.", "contents": "Comparison of the shock-inducing effect of anti-mouse anti-thymocyte horse serum and normal horse serum. I. Experiments with three-week-old mice. Sera administered intravenously to mice hypersensitized with normal horse serum and with horse serum of anti-lymphocytic activity, produce reactions that differ considerably in mice treated in different ways. The experiments of the authors indicate that the anaphylactogenic effect of ATS is substantially stronger than that or normal horse serum. The paper discusses the clinical and theoretical conclusions to be drawn from this phenomenon observed in the experiments."}
{"id": "PMID:1088392", "title": "The sensitizing properties of anti-mouse anti-thymocyte horse serum.", "content": "The authors have compared the anaphylaxis due to active and passive sensitization of mice. In the case of active sensitizing, anti-mouse anti-thymocyte horse serum (ATS), and/or normal horse serum (NHS), whereas in the case of passive sensitizing, plasma, peripheral leukocytes, spleen cells and thymocytes of sensitized animals were used. Provocation of shock was carried out by intravenous administration of ATS or NHS. Irreversible anaphylaxis occurred in a significantly higher rate in the case of ATS than NHS sensitivity, produced either actively, or passively. Differences have been found also between the 4 investigated materials according to their passive transfer potency.", "contents": "The sensitizing properties of anti-mouse anti-thymocyte horse serum. The authors have compared the anaphylaxis due to active and passive sensitization of mice. In the case of active sensitizing, anti-mouse anti-thymocyte horse serum (ATS), and/or normal horse serum (NHS), whereas in the case of passive sensitizing, plasma, peripheral leukocytes, spleen cells and thymocytes of sensitized animals were used. Provocation of shock was carried out by intravenous administration of ATS or NHS. Irreversible anaphylaxis occurred in a significantly higher rate in the case of ATS than NHS sensitivity, produced either actively, or passively. Differences have been found also between the 4 investigated materials according to their passive transfer potency."}
{"id": "PMID:1088393", "title": "Further investigations about the passive transfer of sensitivity caused by anti-thymocyte serum.", "content": "Soluble materials were obtained from the spleens and thymuses removed from mice sensitized by anti-thymocyte serum or normal horse serum. These materials were administered to mice. In the mice thus treated, anaphylaxis was provoked by iv inoculation of serum. Irreversible shock did not occur. Reversible anaphylactoid symptoms occured among the animals treated with the supernatants of cells of sensitized animals, in case when these cells have been disrupted by osmotic shock.", "contents": "Further investigations about the passive transfer of sensitivity caused by anti-thymocyte serum. Soluble materials were obtained from the spleens and thymuses removed from mice sensitized by anti-thymocyte serum or normal horse serum. These materials were administered to mice. In the mice thus treated, anaphylaxis was provoked by iv inoculation of serum. Irreversible shock did not occur. Reversible anaphylactoid symptoms occured among the animals treated with the supernatants of cells of sensitized animals, in case when these cells have been disrupted by osmotic shock."}
{"id": "PMID:1088395", "title": "[Cytological structure of the lymph nodes after administration of foreign protein].", "content": "Changes in the cellular compositon of popliteal lymphatic nodes of albino rats were studied in normality and after injection of a foreign protein. Allergic inflammation was caused by injection of 0,25 ml of the horse serum. Observations were made on the 1, 3, 8, 19 and 30 days after the beginning of experiment. The cellular composition was counted in 30 visual fileds in the cortical plateu, follicles and myelinated cords. The average percentage of cellular elements has been determined. It has been shown that the reticular cells have less changes as compared with other cells in response to antigen stimulation. An inverse correlative relationship was noted between small and medium-sized lymphocytes. The greatest percentage of plasmic cells was noted in myelinated cords; in the follicles they are found as solitary units. In the primary immunization the cellular composition of follicles was little changed as compared with the cellular composition of the cortical and medullary substance. The cellular reaction was most pronounced in myelinated cords. Thus, the investigation of the cellular composition of different structure components of the rat's lymphatic nodes after injection of the foreign protein has revealed a different degree of their reaction.", "contents": "[Cytological structure of the lymph nodes after administration of foreign protein]. Changes in the cellular compositon of popliteal lymphatic nodes of albino rats were studied in normality and after injection of a foreign protein. Allergic inflammation was caused by injection of 0,25 ml of the horse serum. Observations were made on the 1, 3, 8, 19 and 30 days after the beginning of experiment. The cellular composition was counted in 30 visual fileds in the cortical plateu, follicles and myelinated cords. The average percentage of cellular elements has been determined. It has been shown that the reticular cells have less changes as compared with other cells in response to antigen stimulation. An inverse correlative relationship was noted between small and medium-sized lymphocytes. The greatest percentage of plasmic cells was noted in myelinated cords; in the follicles they are found as solitary units. In the primary immunization the cellular composition of follicles was little changed as compared with the cellular composition of the cortical and medullary substance. The cellular reaction was most pronounced in myelinated cords. Thus, the investigation of the cellular composition of different structure components of the rat's lymphatic nodes after injection of the foreign protein has revealed a different degree of their reaction."}
{"id": "PMID:1088398", "title": "The effect of nutritional status on immune capacity and immune responses in preschool children in a rural community in India.", "content": "Cell-mediated immune response (CMI) and several aspects of humoral immune status and response were measured and related to nutritional status in preschool children in north India. CMI was measured by means of postvaccinal (BCG) tuberculin sensitivity and leucocytic blast cell transformation. Humoral immune response was measured by means of tetanus antibody production following vaccination with diphtheria-pertussis-tetanus vaccine. Immunoglobulins A, G, and M and complement (C(3)) were also determined. CMI, serum IgA, and C(3) were found to be directly correlated with weight-for-age status.", "contents": "The effect of nutritional status on immune capacity and immune responses in preschool children in a rural community in India. Cell-mediated immune response (CMI) and several aspects of humoral immune status and response were measured and related to nutritional status in preschool children in north India. CMI was measured by means of postvaccinal (BCG) tuberculin sensitivity and leucocytic blast cell transformation. Humoral immune response was measured by means of tetanus antibody production following vaccination with diphtheria-pertussis-tetanus vaccine. Immunoglobulins A, G, and M and complement (C(3)) were also determined. CMI, serum IgA, and C(3) were found to be directly correlated with weight-for-age status."}
{"id": "PMID:1088401", "title": "An assessment of the comparative advantages of paediatric activities using routine hospital records.", "content": "Comparison is made of the advantages, in terms of benefit to patients and salary costs, of employing a consultant paediatrician and non-specialist physicians in clinical work, management activities, research, and education. Comparisons are based on data derived by a simple method from inpatient records of 10 125 children aged 0-5 years admitted over a 23-month period to Mbale Hospital, Uganda. Benefits to inpatients were assessed from the quarterly death and failure rates and the proportion of failures occurring within 24 h of admission to hospital. The relative costs of the various items of medical work were based on the salary costs that they incurred. The advantages of one activity compared with another are demonstrated, and the results clearly indicate that greater benefit at lower cost was obtained by the use of physicians in management and supervisory work than by employing them in the routine care of inpatients.", "contents": "An assessment of the comparative advantages of paediatric activities using routine hospital records. Comparison is made of the advantages, in terms of benefit to patients and salary costs, of employing a consultant paediatrician and non-specialist physicians in clinical work, management activities, research, and education. Comparisons are based on data derived by a simple method from inpatient records of 10 125 children aged 0-5 years admitted over a 23-month period to Mbale Hospital, Uganda. Benefits to inpatients were assessed from the quarterly death and failure rates and the proportion of failures occurring within 24 h of admission to hospital. The relative costs of the various items of medical work were based on the salary costs that they incurred. The advantages of one activity compared with another are demonstrated, and the results clearly indicate that greater benefit at lower cost was obtained by the use of physicians in management and supervisory work than by employing them in the routine care of inpatients."}
{"id": "PMID:1088402", "title": "Epidemiological features of choriocarcinoma.", "content": "By means of hospital records and death certificates, 91 cases of choriocarcinoma were identified in the cities of Manila, Quezon, Pasay, and Caloocan in the Philippines during the 5 years 1970-74. The overall incidence was 17.4 per 100 000 live births. The other principal findings in this population-based study concerned maternal age, history of fetal wastage, and number of pregnancies. Very high incidence rates for choriocarcinoma were registered for mothers aged 40 years and over and there was a slightly higher than average risk for women under 20 years of age. Risk increased with the number of fetal losses. The effect of number of pregnancies was evident only for very old and very young mothers. A case-control study was also conducted on 28 patients with choriocarcinoma and on 187 age-matched controls; for all of these women, the disease had been diganosed (cases) or children had been born (controls) between 1970 and 1975. Compared with the control group, a higher proportion of the case group had a history of pulmonary tuberculosis and had previously used contraceptive pills. Because of the low and unequal levels of cooperation among patients and controls, these findings need further confirmation.", "contents": "Epidemiological features of choriocarcinoma. By means of hospital records and death certificates, 91 cases of choriocarcinoma were identified in the cities of Manila, Quezon, Pasay, and Caloocan in the Philippines during the 5 years 1970-74. The overall incidence was 17.4 per 100 000 live births. The other principal findings in this population-based study concerned maternal age, history of fetal wastage, and number of pregnancies. Very high incidence rates for choriocarcinoma were registered for mothers aged 40 years and over and there was a slightly higher than average risk for women under 20 years of age. Risk increased with the number of fetal losses. The effect of number of pregnancies was evident only for very old and very young mothers. A case-control study was also conducted on 28 patients with choriocarcinoma and on 187 age-matched controls; for all of these women, the disease had been diganosed (cases) or children had been born (controls) between 1970 and 1975. Compared with the control group, a higher proportion of the case group had a history of pulmonary tuberculosis and had previously used contraceptive pills. Because of the low and unequal levels of cooperation among patients and controls, these findings need further confirmation."}
{"id": "PMID:1088403", "title": "Variability of the diagnosis of stroke by clinical judgement and by a scoring method.", "content": "Existing criteria for the diagnosis of acute cerebrovascular disease (stroke) have not been satisfactory in epidemiological studies. Variability of the diagnosis of stroke, which had not been studied before, was investigated in a WHO collaborative study. Intra-observer and inter-observer variation of the diagnosis of stroke was studied by means of 45 case reports drawn at random from among those included in the study. Diagnosis of stroke and of the type of stroke was made by clinical judgement and by a scoring method. The clinical diagnosis of stroke was more consistent and more comparable than the diagnosis of the type of stroke. Inter-observer agreement in clinical diagnosis was improved by using the score method.", "contents": "Variability of the diagnosis of stroke by clinical judgement and by a scoring method. Existing criteria for the diagnosis of acute cerebrovascular disease (stroke) have not been satisfactory in epidemiological studies. Variability of the diagnosis of stroke, which had not been studied before, was investigated in a WHO collaborative study. Intra-observer and inter-observer variation of the diagnosis of stroke was studied by means of 45 case reports drawn at random from among those included in the study. Diagnosis of stroke and of the type of stroke was made by clinical judgement and by a scoring method. The clinical diagnosis of stroke was more consistent and more comparable than the diagnosis of the type of stroke. Inter-observer agreement in clinical diagnosis was improved by using the score method."}
{"id": "PMID:1088404", "title": "Experience from a multicentre stroke register: a preliminary report.", "content": "In collaboration with 15 centres in 10 countries of Africa, Asia, and Europe, WHO started a pilot study of a community-based stroke register, with standardized methods. Preliminary data were obtained on 6395 new cases of stroke in defined study communities, from May 1971 to September 1974. Information on incidence rates, clinical profiles, diagnosis, management, and course and prognosis for these patients is given.", "contents": "Experience from a multicentre stroke register: a preliminary report. In collaboration with 15 centres in 10 countries of Africa, Asia, and Europe, WHO started a pilot study of a community-based stroke register, with standardized methods. Preliminary data were obtained on 6395 new cases of stroke in defined study communities, from May 1971 to September 1974. Information on incidence rates, clinical profiles, diagnosis, management, and course and prognosis for these patients is given."}
{"id": "PMID:1088405", "title": "Characterization of avian influenza viruses. Designation of a newly recognized haemagglutinin.", "content": "Studies with specific antisera to the haemagglutinin and neuraminidase antigens of all the influenza A subtypes show that A/turkey/Wisconsin/66 influenza virus, originally included in the Hav6 subtype, does not react in either haemagglutinin inhibition or immunodiffusion tests with antisera to Hav6. It is therefore proposed that A/turkey/Wisconsin/66 be placed in a new subtype designated Hav9. The neuraminidase antigens of the Hav6 subtype were further characterized and were shown to be N1, N2, Neq2, and Nav5 subtypes. Hav6 influenza viruses isolated from turkeys over an 11-year period showed little antigenic drift. The haemagglutinin and neuraminidase of A/shearwater/Australia/72 (Hav6Nav5) were identical with those of a virus isolated 8 years previously from a turkey in California: A/turkey/California/64 (Hav6Nav5).", "contents": "Characterization of avian influenza viruses. Designation of a newly recognized haemagglutinin. Studies with specific antisera to the haemagglutinin and neuraminidase antigens of all the influenza A subtypes show that A/turkey/Wisconsin/66 influenza virus, originally included in the Hav6 subtype, does not react in either haemagglutinin inhibition or immunodiffusion tests with antisera to Hav6. It is therefore proposed that A/turkey/Wisconsin/66 be placed in a new subtype designated Hav9. The neuraminidase antigens of the Hav6 subtype were further characterized and were shown to be N1, N2, Neq2, and Nav5 subtypes. Hav6 influenza viruses isolated from turkeys over an 11-year period showed little antigenic drift. The haemagglutinin and neuraminidase of A/shearwater/Australia/72 (Hav6Nav5) were identical with those of a virus isolated 8 years previously from a turkey in California: A/turkey/California/64 (Hav6Nav5)."}
{"id": "PMID:1088406", "title": "A large-scale snail control trial with trifenmorph in the Gezira irrigation scheme, Sudan.", "content": "A large-scale field trial was carried out during December 1973 to assess the effect of trifenmorph on Bulinus truncatus and Biomphalaria pfeifferi in 379 000 feddans ( approximately 159 000 ha) of the Gezira irrigation system in the Sudan. The commercial formulation used (Frescon) is an emulsifiable concentrate containing 16.5% trifenmorph. Five dispensers were used to add the commercial product to the water continuously for 7.5 days; 18 121 litres were used to treat 28.4 million m(3) of water. In addition, each minor canal was hand-sprayed from the tail to 300 m upstream of the last open field outlet pipe; 360 litres of the commercial formulation were used for this operation.A minimum concentration of 0.035 mg trifenmorph per litre of water was produced at the head of each minor canal. The use of caged snails showed that a concentration as low as 0.015 mg/litre was sufficient to produce 100% mortality in B. truncatus in 7.5 days; this is equivalent to a concentration x time product of 0.12 mg/litre days.", "contents": "A large-scale snail control trial with trifenmorph in the Gezira irrigation scheme, Sudan. A large-scale field trial was carried out during December 1973 to assess the effect of trifenmorph on Bulinus truncatus and Biomphalaria pfeifferi in 379 000 feddans ( approximately 159 000 ha) of the Gezira irrigation system in the Sudan. The commercial formulation used (Frescon) is an emulsifiable concentrate containing 16.5% trifenmorph. Five dispensers were used to add the commercial product to the water continuously for 7.5 days; 18 121 litres were used to treat 28.4 million m(3) of water. In addition, each minor canal was hand-sprayed from the tail to 300 m upstream of the last open field outlet pipe; 360 litres of the commercial formulation were used for this operation.A minimum concentration of 0.035 mg trifenmorph per litre of water was produced at the head of each minor canal. The use of caged snails showed that a concentration as low as 0.015 mg/litre was sufficient to produce 100% mortality in B. truncatus in 7.5 days; this is equivalent to a concentration x time product of 0.12 mg/litre days."}
{"id": "PMID:1088407", "title": "A socioecological approach to the control of Schistosoma mansoni in St Lucia.", "content": "Systematic observations of man/water contact in a valley endemic for S. mansoni in St Lucia were carried out prior to the introduction of a household water supply. The observations indicated that exposure of the population to cercaria-infested rivers and streams could be broken down into different types related to distinct and measurable domestic and economic activities. The number and duration of daily contacts with water played an important role in determining the relative risk of infection and correlated significantly with the number of infected persons by age in a survey conducted immediately following the observations. Consideration should be given to measuring and assessing the relative importance of different activities involving contact with water in relation to transmission and the formulation of control strategies.", "contents": "A socioecological approach to the control of Schistosoma mansoni in St Lucia. Systematic observations of man/water contact in a valley endemic for S. mansoni in St Lucia were carried out prior to the introduction of a household water supply. The observations indicated that exposure of the population to cercaria-infested rivers and streams could be broken down into different types related to distinct and measurable domestic and economic activities. The number and duration of daily contacts with water played an important role in determining the relative risk of infection and correlated significantly with the number of infected persons by age in a survey conducted immediately following the observations. Consideration should be given to measuring and assessing the relative importance of different activities involving contact with water in relation to transmission and the formulation of control strategies."}
{"id": "PMID:1088408", "title": "Cellular alterations accompany opacification in the cultured lens.", "content": "An examination of frog lenses cultured in specific serum-enriched medium was undertaken in order to determine whether such lenses could serve as in vitro models for studying the role of the lens epithelial cell. Histological analysis after sectioning of the lens revealed multilayered epithelial plaques and epithelial invasion of vacuolated cortical fibres, accompanied by the deposition of capsule-like material. A comparison of the effects of two media, L-15 and 199, indicated a greater incidence of opacification induced by L-15, which may be correlated with changes in lens epithelial cell nuclei.", "contents": "Cellular alterations accompany opacification in the cultured lens. An examination of frog lenses cultured in specific serum-enriched medium was undertaken in order to determine whether such lenses could serve as in vitro models for studying the role of the lens epithelial cell. Histological analysis after sectioning of the lens revealed multilayered epithelial plaques and epithelial invasion of vacuolated cortical fibres, accompanied by the deposition of capsule-like material. A comparison of the effects of two media, L-15 and 199, indicated a greater incidence of opacification induced by L-15, which may be correlated with changes in lens epithelial cell nuclei."}
{"id": "PMID:1088414", "title": "Patterns of B-lymphocyte gene expression elicited by lipopolysaccharide mitogen.", "content": "When large proportions of B lymphocytes from the murine spleen are stimulated in vitro by bacterial lipopolysaccharide (LPS) B lymphoblasts with small amounts of intracellular immunoglobulin (Ig) and plasmablasts with large amounts of intracellular Ig concomitantly proliferate. It is likely that B lymphocytes are heterogeneous and LPS activates B cells to express their predetermined functional capacity since bromodeoxyuridine does not inhibit the initiation of Ig synthesis in plasmablasts, and Ig synthesis starts before these cells complete their first mitosis. The results suggest that LPS is a potent polyclonal activator (of a B-cell subset) but it is not a differentiation factor in the sense that it is unable to determine whether its target cell develops extensive endoplasmic reticulum or follows a different pathway. The results do not exclude that modulation of B cells' genetic programming might take place during T cell-dependent B-lymphocyte activation. The observed B-cell heterogeneity offers a possible explanation for the concomitant emergence of B memory cells and antibody producers during the early phase of immune responses in vivo.", "contents": "Patterns of B-lymphocyte gene expression elicited by lipopolysaccharide mitogen. When large proportions of B lymphocytes from the murine spleen are stimulated in vitro by bacterial lipopolysaccharide (LPS) B lymphoblasts with small amounts of intracellular immunoglobulin (Ig) and plasmablasts with large amounts of intracellular Ig concomitantly proliferate. It is likely that B lymphocytes are heterogeneous and LPS activates B cells to express their predetermined functional capacity since bromodeoxyuridine does not inhibit the initiation of Ig synthesis in plasmablasts, and Ig synthesis starts before these cells complete their first mitosis. The results suggest that LPS is a potent polyclonal activator (of a B-cell subset) but it is not a differentiation factor in the sense that it is unable to determine whether its target cell develops extensive endoplasmic reticulum or follows a different pathway. The results do not exclude that modulation of B cells' genetic programming might take place during T cell-dependent B-lymphocyte activation. The observed B-cell heterogeneity offers a possible explanation for the concomitant emergence of B memory cells and antibody producers during the early phase of immune responses in vivo."}
{"id": "PMID:1088415", "title": "Antigenic properties of subsets of splenic T lymphocytes responding to lectins.", "content": "The aim of the present study was to characterize the antigenic properties of spleen cells responding in vitro to various mitogenic stimuli. Mouse spleen cells were treated with alloantibodies and C and then cultured with various concentrations of lectins. The exposure of spleen cells to various dilutions of either anti-theta serum and C or anti-Ly serum and C inhibited the response to optimal concentrations of PHA to a greater extent than the response to optimal concentrations of Con A. With the exception of the optimal lectin concentration, theta-antiserum and C had a stronger inhibitory effect on the response to Con A than to PHA. The exposure of spleen cells to H-2 antiserum and C inhibited their response to Con A to a greater extent than to PHA. The inhibitory activity of H-2 antiserum and C was inversely correlated with the dose of PHA used for stimulation. The lower the concentration of PHA used for stimulation the more effective was the inhibitory effect of the antiserum. The inhibitory effect of H-2 antiserum and C on the response to Con A was highest at optimal concentrations of the mitogen, and somewhat less pronounced above or below this concentration. The present study suggests that subsets of splenic T-cells that react to various concentrations of mitogens, differ in their theta- and H-2 alloantigenicity.", "contents": "Antigenic properties of subsets of splenic T lymphocytes responding to lectins. The aim of the present study was to characterize the antigenic properties of spleen cells responding in vitro to various mitogenic stimuli. Mouse spleen cells were treated with alloantibodies and C and then cultured with various concentrations of lectins. The exposure of spleen cells to various dilutions of either anti-theta serum and C or anti-Ly serum and C inhibited the response to optimal concentrations of PHA to a greater extent than the response to optimal concentrations of Con A. With the exception of the optimal lectin concentration, theta-antiserum and C had a stronger inhibitory effect on the response to Con A than to PHA. The exposure of spleen cells to H-2 antiserum and C inhibited their response to Con A to a greater extent than to PHA. The inhibitory activity of H-2 antiserum and C was inversely correlated with the dose of PHA used for stimulation. The lower the concentration of PHA used for stimulation the more effective was the inhibitory effect of the antiserum. The inhibitory effect of H-2 antiserum and C on the response to Con A was highest at optimal concentrations of the mitogen, and somewhat less pronounced above or below this concentration. The present study suggests that subsets of splenic T-cells that react to various concentrations of mitogens, differ in their theta- and H-2 alloantigenicity."}
{"id": "PMID:1088416", "title": "Development of B-lymphocyte colony-forming cells in foetal mouse tissues.", "content": "In CBA and (CBA X C57B1)F1 mice, cells forming B-lymphocyte colonies in agar culture were first detected in the 17-day foetal liver and the following day in the spleen, bone marrow and peripheral blood. Colony-forming cells were not detected in the yolk sac or foetal thymus. Adult levels of colony-forming cells were achieved within 3 days of birth. In organ cultures of 15-day foetal liver or spleen, B-lymphocyte colony-forming cells developed during a 5-day incubation period, indicating that both organs can function as bursal analogues. Foetal liver colony-forming cells were of small size and generated colonies of cells with a pattern of membrane immunoglobulin similar to colony cells generated by cells from adult animals.", "contents": "Development of B-lymphocyte colony-forming cells in foetal mouse tissues. In CBA and (CBA X C57B1)F1 mice, cells forming B-lymphocyte colonies in agar culture were first detected in the 17-day foetal liver and the following day in the spleen, bone marrow and peripheral blood. Colony-forming cells were not detected in the yolk sac or foetal thymus. Adult levels of colony-forming cells were achieved within 3 days of birth. In organ cultures of 15-day foetal liver or spleen, B-lymphocyte colony-forming cells developed during a 5-day incubation period, indicating that both organs can function as bursal analogues. Foetal liver colony-forming cells were of small size and generated colonies of cells with a pattern of membrane immunoglobulin similar to colony cells generated by cells from adult animals."}
{"id": "PMID:1088411", "title": "[Chloroderivatives of phenoxyacetic acid as antagonists of thyroid hormones].", "content": "A 24-hour stay of the Rana temporaria tadpoles in solutions of the sodium and diethylamine salts of 2,4-dichlorophenoxyacitic acid (with 1 and 2 mg/l, respectively) significantly inhibits their metamorphosis. An addition of these salts to a solution containing thyroidin in a concentration of 1--5 mg/l hinders the action of hormone stimulating metamorphosis. The effect of the sodium salt of 2,4-dichlorophenoxyacetic acid prevents the destruction of some tadpoles by toxic concentrations of thyroidin. The above data justify referring the chloroderivatives of the phenoxyacetic acid to antagonists of the thyroid hormones.", "contents": "[Chloroderivatives of phenoxyacetic acid as antagonists of thyroid hormones]. A 24-hour stay of the Rana temporaria tadpoles in solutions of the sodium and diethylamine salts of 2,4-dichlorophenoxyacitic acid (with 1 and 2 mg/l, respectively) significantly inhibits their metamorphosis. An addition of these salts to a solution containing thyroidin in a concentration of 1--5 mg/l hinders the action of hormone stimulating metamorphosis. The effect of the sodium salt of 2,4-dichlorophenoxyacetic acid prevents the destruction of some tadpoles by toxic concentrations of thyroidin. The above data justify referring the chloroderivatives of the phenoxyacetic acid to antagonists of the thyroid hormones."}
{"id": "PMID:1088417", "title": "Cytotoxicity of normal rabbit serum for chicken lymphoid cells. I. Localization of the activity.", "content": "Normal rabbit sera were found to be highly cytotoxic for chicken thymus cells. When the sera were fractionated on Sephadex G-200, the natural cytotoixc activity resided exclusively in the first peak. It was found to be complement-dependent and heat stable at 56 degrees. On the other hand, in the serum of immunized animals the specific cytotoxic activity appeared in the second peak.", "contents": "Cytotoxicity of normal rabbit serum for chicken lymphoid cells. I. Localization of the activity. Normal rabbit sera were found to be highly cytotoxic for chicken thymus cells. When the sera were fractionated on Sephadex G-200, the natural cytotoixc activity resided exclusively in the first peak. It was found to be complement-dependent and heat stable at 56 degrees. On the other hand, in the serum of immunized animals the specific cytotoxic activity appeared in the second peak."}
{"id": "PMID:1088418", "title": "The effect of cyclophosphamide on the recovery from a local chlamydial infection. Guinea-pig inclusion conjunctivitis (GPIC).", "content": "The immune mechanism involved in the recovery from and resistance to guinea-pig inclusion conjunctivitis (GPIC) was studied. Guinea-pigs were injected with a dose of cyclophosphamide (CY) (300 mg/kg wt) that inhibits antibody synthesis. Such treatment was shown to produce a cellular depletion in the B-cell area without producing an appreciable change in the T-cell area of the spleen and lymph nodes. CY treatment markedly delayed the appearance of secretory immunoglobulin A antibody to GPIC in the tears, and other classes of antibodies to GPIC and sheep erythrocyte in the serum. Furthermore, recovery from infection was impaired and a subsequent injection of CY prolonged the duration of infection. The results indicate that B cells may play an important role in the control of this infection.", "contents": "The effect of cyclophosphamide on the recovery from a local chlamydial infection. Guinea-pig inclusion conjunctivitis (GPIC). The immune mechanism involved in the recovery from and resistance to guinea-pig inclusion conjunctivitis (GPIC) was studied. Guinea-pigs were injected with a dose of cyclophosphamide (CY) (300 mg/kg wt) that inhibits antibody synthesis. Such treatment was shown to produce a cellular depletion in the B-cell area without producing an appreciable change in the T-cell area of the spleen and lymph nodes. CY treatment markedly delayed the appearance of secretory immunoglobulin A antibody to GPIC in the tears, and other classes of antibodies to GPIC and sheep erythrocyte in the serum. Furthermore, recovery from infection was impaired and a subsequent injection of CY prolonged the duration of infection. The results indicate that B cells may play an important role in the control of this infection."}
{"id": "PMID:1088419", "title": "Rabbit lymphoid cells. II. Anti-allotype antisera, lipopolysaccharide and other bacterial and fungal mitogens as probes for the identification of B-cell subpopulations.", "content": "Removal of adherent cells or complement-mediated killing of rabbit thymus lymphocyte antigen (BTLA) bearing rabbit T lymphocytes did not abolish the responsiveness (increased thymidine incorporation) of lymphoid cells to antibody against immunoglobulin allotype, Nocardia water-soluble mitogen (NWSM), pneumococcal polysaccharide SIII (PPSIII), S. abortus lipopolysaccharide (LPS), lipid A conjugated to bovine serum albumin and a crude preparation containing C polysaccharide from the cell wall of Diplococcus pneumoniae. Isologous and heterologous antisera, directed against different portions of the Ig receptor, differed in their capacity to enhance thymidine incorporation. The difference in mitogenicity of these antisera was discussed in terms of the accessibility of cell-bound immunoglobulin receptor sites. Spleen cells, responsive to anti-allotype (Ab4) antiserum and B-cell mitogens, NWSM and PPSIII, were characterized by velocity sedimentation. The mean volume of NWSM- and PPSIII-responsive cells was larger than that of the cells responsive to anti-allotype antiserum. Fractionation of spleen cells on glass bead columns yielded a population of non-adherent cells which were two to six times as responsive to anti-Ab4 antiserum as the original spleen cell suspension. The responsiveness of peripheral blood lymphocytes to anti-Ab4 antiserum was significantly greater than that of spleen cells. On the other hand, spleen cells were more responsive to PPSIII than were cells from the peripheral blood, the popliteal and the mesenteric lymph nodes.", "contents": "Rabbit lymphoid cells. II. Anti-allotype antisera, lipopolysaccharide and other bacterial and fungal mitogens as probes for the identification of B-cell subpopulations. Removal of adherent cells or complement-mediated killing of rabbit thymus lymphocyte antigen (BTLA) bearing rabbit T lymphocytes did not abolish the responsiveness (increased thymidine incorporation) of lymphoid cells to antibody against immunoglobulin allotype, Nocardia water-soluble mitogen (NWSM), pneumococcal polysaccharide SIII (PPSIII), S. abortus lipopolysaccharide (LPS), lipid A conjugated to bovine serum albumin and a crude preparation containing C polysaccharide from the cell wall of Diplococcus pneumoniae. Isologous and heterologous antisera, directed against different portions of the Ig receptor, differed in their capacity to enhance thymidine incorporation. The difference in mitogenicity of these antisera was discussed in terms of the accessibility of cell-bound immunoglobulin receptor sites. Spleen cells, responsive to anti-allotype (Ab4) antiserum and B-cell mitogens, NWSM and PPSIII, were characterized by velocity sedimentation. The mean volume of NWSM- and PPSIII-responsive cells was larger than that of the cells responsive to anti-allotype antiserum. Fractionation of spleen cells on glass bead columns yielded a population of non-adherent cells which were two to six times as responsive to anti-Ab4 antiserum as the original spleen cell suspension. The responsiveness of peripheral blood lymphocytes to anti-Ab4 antiserum was significantly greater than that of spleen cells. On the other hand, spleen cells were more responsive to PPSIII than were cells from the peripheral blood, the popliteal and the mesenteric lymph nodes."}
{"id": "PMID:1088420", "title": "The relation between the cell-mediated immunological response and the induction of circulating antibodies to collagen in guinea-pigs.", "content": "Cutaneous delayed hypersensitivity reactions to collagen in guinea-pigs were partially but specifically suppressed if the animals had been pretreated with collagen and Freund's incomplete adjuvant. Such animals responded normally to skin-reactive factor prepared with ovalbumin. Lymphoid cells from animals with normal delayed hypersensitivity to collagen functioned normally in animals with suppressed skin reactivity. Cells from animals with suppressed delayed hypersensitivity were specifically, functionally impaired since they transferred delayed hypersensitivity into neutral recipients efficiently for PPD but not for collagen. Suppression could be induced in Cy-treated animals, and it persisted for at least 143 days. It is concluded that guinea-pigs with depressed delayed hypersensitivity to collagen are functionally impaired with respect to those T cells normally generated by induction of delayed hypersensitivity.", "contents": "The relation between the cell-mediated immunological response and the induction of circulating antibodies to collagen in guinea-pigs. Cutaneous delayed hypersensitivity reactions to collagen in guinea-pigs were partially but specifically suppressed if the animals had been pretreated with collagen and Freund's incomplete adjuvant. Such animals responded normally to skin-reactive factor prepared with ovalbumin. Lymphoid cells from animals with normal delayed hypersensitivity to collagen functioned normally in animals with suppressed skin reactivity. Cells from animals with suppressed delayed hypersensitivity were specifically, functionally impaired since they transferred delayed hypersensitivity into neutral recipients efficiently for PPD but not for collagen. Suppression could be induced in Cy-treated animals, and it persisted for at least 143 days. It is concluded that guinea-pigs with depressed delayed hypersensitivity to collagen are functionally impaired with respect to those T cells normally generated by induction of delayed hypersensitivity."}
{"id": "PMID:1088409", "title": "[Electroencephalographic analysis of the central action of pyrazidol].", "content": "Pyrasidol (1,10-trimethylene-8-methyl-1,2,3,4-tetrahydropyrasino/1,2-q/indol hydrochloride) produces an activating influence on the EEG of the brain cortex, the reticular formation of the mesencephalon and hippocampus. It potentiates the electroencephalographic effects of amphetamine, 1-DOPA and 5-oxytryptophan, displays no central cholinolytic activity and has no influence on the electrophysiological effects of anticholinesterase and cholinomimetic agents.", "contents": "[Electroencephalographic analysis of the central action of pyrazidol]. Pyrasidol (1,10-trimethylene-8-methyl-1,2,3,4-tetrahydropyrasino/1,2-q/indol hydrochloride) produces an activating influence on the EEG of the brain cortex, the reticular formation of the mesencephalon and hippocampus. It potentiates the electroencephalographic effects of amphetamine, 1-DOPA and 5-oxytryptophan, displays no central cholinolytic activity and has no influence on the electrophysiological effects of anticholinesterase and cholinomimetic agents."}
{"id": "PMID:1088421", "title": "The immune response to oxidized ferredoxin. II. Cross reactivity of cells and antisera to modified ferredoxins and the nature of the cells responding in vitro.", "content": "The cross reactivity of sera from rabbits sensitized to performic acid oxidized ferredoxin (O-Fd) and of spleen cells from mice sensitized to O-Fd was analysed using several chemically modified forms of ferredoxin in the complement fixation test and the in vitro lymphocyte stimulation assay. Only O-Fd and native ferredoxin (native-Fd) gave positive responses in both assays. Dinitrophenylated-O-Fd (DNP-O-Fd) and acid precipitated ferredoxin (TCA-Fd) were able to fix complement (C') but did not simulate DNA synthesis in vitro. Ferredoxin alkylated with N-ethylmaleimioxin (CM-Fd) was unable to stimulate DNA synthesis and was marginally able to fix C'. Methylated-O-Fd (meth-O-Fd) was not recognized in either assay. The various ferredoxin preparations were tested for their ability to sensitize mice for use in the in vitro lymphocyte stimulation assay. Only O-Fd, NEM-Fd and native-Fd were capable of sensitizing lymphocytes for a proliferative response in vitro to the test antigens. This correlates with the observation that only these antigens were able to induce DNA synthesis in O-Fd-sensitized lymphocytes. The nature of the cells responding in vitro was examined by treating the cells with rabbit anti-mouse immunoglobulin and C' or rabbit anti-mouse brain associated theta and C'. The 24-hr response was found to be sensitive to both sera while the 120-hr response was sensitive only to the anti-theta sera.", "contents": "The immune response to oxidized ferredoxin. II. Cross reactivity of cells and antisera to modified ferredoxins and the nature of the cells responding in vitro. The cross reactivity of sera from rabbits sensitized to performic acid oxidized ferredoxin (O-Fd) and of spleen cells from mice sensitized to O-Fd was analysed using several chemically modified forms of ferredoxin in the complement fixation test and the in vitro lymphocyte stimulation assay. Only O-Fd and native ferredoxin (native-Fd) gave positive responses in both assays. Dinitrophenylated-O-Fd (DNP-O-Fd) and acid precipitated ferredoxin (TCA-Fd) were able to fix complement (C') but did not simulate DNA synthesis in vitro. Ferredoxin alkylated with N-ethylmaleimioxin (CM-Fd) was unable to stimulate DNA synthesis and was marginally able to fix C'. Methylated-O-Fd (meth-O-Fd) was not recognized in either assay. The various ferredoxin preparations were tested for their ability to sensitize mice for use in the in vitro lymphocyte stimulation assay. Only O-Fd, NEM-Fd and native-Fd were capable of sensitizing lymphocytes for a proliferative response in vitro to the test antigens. This correlates with the observation that only these antigens were able to induce DNA synthesis in O-Fd-sensitized lymphocytes. The nature of the cells responding in vitro was examined by treating the cells with rabbit anti-mouse immunoglobulin and C' or rabbit anti-mouse brain associated theta and C'. The 24-hr response was found to be sensitive to both sera while the 120-hr response was sensitive only to the anti-theta sera."}
{"id": "PMID:1088410", "title": "[Antifibrinolytic and antimycotic properties of 2-thiontetrahydro-1,3,5-thiadiazine].", "content": "A series of tetrahydro-1,3,5-thiadiazine-2-thiones have been tested for their fungistatic and antifibrinolytic effects. The fungistatic activity depends upon the substitution of nitrogen in positions 3 and 5 of the ring system. But it is, however, only the derivatives that are able to split off the antifibrinolytically active w-amino acids by solvolysis which produces antifibrinolytic effect in vivo.", "contents": "[Antifibrinolytic and antimycotic properties of 2-thiontetrahydro-1,3,5-thiadiazine]. A series of tetrahydro-1,3,5-thiadiazine-2-thiones have been tested for their fungistatic and antifibrinolytic effects. The fungistatic activity depends upon the substitution of nitrogen in positions 3 and 5 of the ring system. But it is, however, only the derivatives that are able to split off the antifibrinolytically active w-amino acids by solvolysis which produces antifibrinolytic effect in vivo."}
{"id": "PMID:1088422", "title": "Differentiation of lymphoid cells: the non-mitogenic induction of immunoglobulin production by thymus cell extract and thymus cell culture filtrate.", "content": "The cell-free medium in which thymocytes have been cultured (filtrate) as well as sonic lysates of thymocytes (extract) enhance immunoglobulin production when added to spleen cells during tissue culture. In spite of the requirement for foetal calf serum in the culture medium, production of the enhancing factor in thymocyte culture filtrates occurred even in the presence of a variety of metabolic inhibitors including NaN3, puromycin and hydroxyurea. Although DNA synthesis is required as a prelude to the induction of immunoglobulin production, two lines of evidence indicate that the enhancement produced in response to filtrate and extract occurs via a non-mitogenic process. First, neither cell-free agent was mitogenic toward spleen cells. Secondly, the enhancement of immunoglobulin production due to filtrate or extract was observed even in the presence of inhibitors of DNA synthesis. Multiple functions for thymocytes in the induction of immunoglobulin production are indicated by the findings that thymocytes restore immunoglobulin production of anti-thymocyte serum-treated spleen cells, whereas filtrate and extract, alone or in combination, do not have this capability. Furthermore, filtrate and extract failed to enhance the induction of DNP-group-specific antibody production by cells incubated with DNP-protein, but filtrate and extract could partially restore anti-DNP antibody production of such anti-thymocyte serum-treated cells. The role of thymocytes, filtrate and extract in the antigen-independent and the antigen-dependent induction of immunoglobulin production is discussed.", "contents": "Differentiation of lymphoid cells: the non-mitogenic induction of immunoglobulin production by thymus cell extract and thymus cell culture filtrate. The cell-free medium in which thymocytes have been cultured (filtrate) as well as sonic lysates of thymocytes (extract) enhance immunoglobulin production when added to spleen cells during tissue culture. In spite of the requirement for foetal calf serum in the culture medium, production of the enhancing factor in thymocyte culture filtrates occurred even in the presence of a variety of metabolic inhibitors including NaN3, puromycin and hydroxyurea. Although DNA synthesis is required as a prelude to the induction of immunoglobulin production, two lines of evidence indicate that the enhancement produced in response to filtrate and extract occurs via a non-mitogenic process. First, neither cell-free agent was mitogenic toward spleen cells. Secondly, the enhancement of immunoglobulin production due to filtrate or extract was observed even in the presence of inhibitors of DNA synthesis. Multiple functions for thymocytes in the induction of immunoglobulin production are indicated by the findings that thymocytes restore immunoglobulin production of anti-thymocyte serum-treated spleen cells, whereas filtrate and extract, alone or in combination, do not have this capability. Furthermore, filtrate and extract failed to enhance the induction of DNP-group-specific antibody production by cells incubated with DNP-protein, but filtrate and extract could partially restore anti-DNP antibody production of such anti-thymocyte serum-treated cells. The role of thymocytes, filtrate and extract in the antigen-independent and the antigen-dependent induction of immunoglobulin production is discussed."}
{"id": "PMID:1088446", "title": "Analgesia induced by electrical stimulation of the inferior centralis nucleus of the raphe in the cat.", "content": "In the cat, electrical stimulation of the inferior central nucleus of the raphe induces a powerful analgesia. This stimulation totally suppresses the behavioural reactions elicited by strong pinches applied to the tail or to the four limbs; it strongly modifies the threshold of the jaw opening reflex obtained by tooth pulp stimulation and considerably affects the behavioural reactions elicited by continuing such stimulation. The results can be considered as evidence that the mechanism of analgesia from the inferior raphe nucleus is similar to that already described in the dorsal raphe nucleus. The analgesia obtained by stimulation of raphe nuclei seems to be sustained by serotoninergic mechanisms and relationships between these are discussed. In preliminary experiments, analgesia induced by CI stimulation has been suppressed by administration of naloxone, a specific opiate antagonist.", "contents": "Analgesia induced by electrical stimulation of the inferior centralis nucleus of the raphe in the cat. In the cat, electrical stimulation of the inferior central nucleus of the raphe induces a powerful analgesia. This stimulation totally suppresses the behavioural reactions elicited by strong pinches applied to the tail or to the four limbs; it strongly modifies the threshold of the jaw opening reflex obtained by tooth pulp stimulation and considerably affects the behavioural reactions elicited by continuing such stimulation. The results can be considered as evidence that the mechanism of analgesia from the inferior raphe nucleus is similar to that already described in the dorsal raphe nucleus. The analgesia obtained by stimulation of raphe nuclei seems to be sustained by serotoninergic mechanisms and relationships between these are discussed. In preliminary experiments, analgesia induced by CI stimulation has been suppressed by administration of naloxone, a specific opiate antagonist."}
{"id": "PMID:1088447", "title": "Influence on touch, vibration and cutaneous pain of dorsal column stimulation in man.", "content": "Five patients out of a group of ten who had dorsal column electrodes implanted for the relief of chronic pain were examined for the influence of the stimulation on the spontaneous pain and on the thresholds for touch, vibration and cutaneous pain induced by pinching. Stimulation producing paraesthesias resulted in an almost immediate abolishment of spontaneous pain and was accompanied by significant elevations of both tactile and vibratory thresholds. Elevation of thresholds was confined to segments below the site of implantation and occurred bilaterally also when the paraesthesias were restricted to one side. The changes of thresholds generally persisted for some time after the stimulation but these effects were short lasting in comparison with the effect on spontaneous pain. Elevation of sensory thresholds is presumably not due to blocking of the primary neurones but to central inhibitory mechanisms. The thresholds for induced cutaneous pain were not influenced by dorsal column stimulation except for one case in whom an abnormally low threshold within an hyperaestethic area became normalized.", "contents": "Influence on touch, vibration and cutaneous pain of dorsal column stimulation in man. Five patients out of a group of ten who had dorsal column electrodes implanted for the relief of chronic pain were examined for the influence of the stimulation on the spontaneous pain and on the thresholds for touch, vibration and cutaneous pain induced by pinching. Stimulation producing paraesthesias resulted in an almost immediate abolishment of spontaneous pain and was accompanied by significant elevations of both tactile and vibratory thresholds. Elevation of thresholds was confined to segments below the site of implantation and occurred bilaterally also when the paraesthesias were restricted to one side. The changes of thresholds generally persisted for some time after the stimulation but these effects were short lasting in comparison with the effect on spontaneous pain. Elevation of sensory thresholds is presumably not due to blocking of the primary neurones but to central inhibitory mechanisms. The thresholds for induced cutaneous pain were not influenced by dorsal column stimulation except for one case in whom an abnormally low threshold within an hyperaestethic area became normalized."}
{"id": "PMID:1088445", "title": "Pharmacological analysis of slow potentials recorded in frog olfactory bulb during natural stimulation.", "content": "Pharmacological agents (strychnine, picrotoxin, pentobarbital, chloralose, GABA, penicillin, morphine) were used to investigate the nature of the slow potential recorded in the frog olfactory bulb in response to natural stimulation. Three possible hypotheses were tested: 1) The slow potential is neuroglial in nature; 2) it is the analog of the dorsal-root potential of the spinal cord and reflects depolarization of primary afferents arising in the terminals of the olfactory nerve and responsible for presynaptic inhibition in the frog olfactory bulb; 3) the slow potential reflects postsynaptic processes. The results showed great similarity between changes in the slow and dorsal-root potentials of the spinal cord in response to the action of pharmacological agents. However, the slow potential is evidently a complex response and incorporates at least one other component - depolarization of the dendrites of unknown nature.", "contents": "Pharmacological analysis of slow potentials recorded in frog olfactory bulb during natural stimulation. Pharmacological agents (strychnine, picrotoxin, pentobarbital, chloralose, GABA, penicillin, morphine) were used to investigate the nature of the slow potential recorded in the frog olfactory bulb in response to natural stimulation. Three possible hypotheses were tested: 1) The slow potential is neuroglial in nature; 2) it is the analog of the dorsal-root potential of the spinal cord and reflects depolarization of primary afferents arising in the terminals of the olfactory nerve and responsible for presynaptic inhibition in the frog olfactory bulb; 3) the slow potential reflects postsynaptic processes. The results showed great similarity between changes in the slow and dorsal-root potentials of the spinal cord in response to the action of pharmacological agents. However, the slow potential is evidently a complex response and incorporates at least one other component - depolarization of the dendrites of unknown nature."}
{"id": "PMID:1088449", "title": "Effect of a proteinase inhibitor on intermittent claudication or on pain at rest in patients with peripheral arterial disease.", "content": "Twenty patients with peripheral arterial disease and 10 normal controls were submitted to i.v. injection of aprotinin, polypeptide (mol.wt. 6512) extracted from bovine lung, in order to examine its effects on: (a) lower limbs pain, (b) lower limbs sensibility, (c) calf blood flow. Aprotinin (100,000 Ku i.v. diluted in NaCl  0.9%) was given in a single dose or twice a day for a week; for control the same subject received, before or after aprotinin, an equivalent volume of diluent (0.9% NaCl). The results demonstrate that aprotinin is able to increase the initial pain limit walking tolerance and to decrease the intensity of pain at rest and of myalgic or \"trigger\" areas. No variation was observed on skin sensibility and on calf blood flow, both basal resting and hyperemic. The favorable effect of examined polypeptide on ischemic pain can be attributed neither to increase of calf blood flow nor to influence on perception of painful stimuli. It seems therefore to suggest that aprotinin acts on biochemical mechanisms that cause the ischemic pain. Presumably it inhibits kininogenases and tissue protein-hydrolyzine enzymes activated in the course of ischemia.", "contents": "Effect of a proteinase inhibitor on intermittent claudication or on pain at rest in patients with peripheral arterial disease. Twenty patients with peripheral arterial disease and 10 normal controls were submitted to i.v. injection of aprotinin, polypeptide (mol.wt. 6512) extracted from bovine lung, in order to examine its effects on: (a) lower limbs pain, (b) lower limbs sensibility, (c) calf blood flow. Aprotinin (100,000 Ku i.v. diluted in NaCl  0.9%) was given in a single dose or twice a day for a week; for control the same subject received, before or after aprotinin, an equivalent volume of diluent (0.9% NaCl). The results demonstrate that aprotinin is able to increase the initial pain limit walking tolerance and to decrease the intensity of pain at rest and of myalgic or \"trigger\" areas. No variation was observed on skin sensibility and on calf blood flow, both basal resting and hyperemic. The favorable effect of examined polypeptide on ischemic pain can be attributed neither to increase of calf blood flow nor to influence on perception of painful stimuli. It seems therefore to suggest that aprotinin acts on biochemical mechanisms that cause the ischemic pain. Presumably it inhibits kininogenases and tissue protein-hydrolyzine enzymes activated in the course of ischemia."}
{"id": "PMID:1088450", "title": "Transcutaneous electrical analgesia: a follow-up analysis.", "content": "One year follow-up data are analyzed for the effects of using transcutaneous neurostimulators on patients with chronic benign pain. Those who have successful surgery for pain relief have lower pain and analgesic intake levels than those who supplement their surgery with neurostimulation. Those who do not receive surgery for pain but use neurostimulators have greatly increased activity levels than those who do not use these devices. The neurostimulators lower the clinical pain level component of the tourniquet test score for non-surgery patients to a degree comparable to that of patients with successful surgical outcomes, but maximum pain tolerance is not significantly altered. This supports the hypothesis that the analgesic effect is primarily a peripheral one.", "contents": "Transcutaneous electrical analgesia: a follow-up analysis. One year follow-up data are analyzed for the effects of using transcutaneous neurostimulators on patients with chronic benign pain. Those who have successful surgery for pain relief have lower pain and analgesic intake levels than those who supplement their surgery with neurostimulation. Those who do not receive surgery for pain but use neurostimulators have greatly increased activity levels than those who do not use these devices. The neurostimulators lower the clinical pain level component of the tourniquet test score for non-surgery patients to a degree comparable to that of patients with successful surgical outcomes, but maximum pain tolerance is not significantly altered. This supports the hypothesis that the analgesic effect is primarily a peripheral one."}
{"id": "PMID:1088456", "title": "Tumor-host cell hybrids in radiochimeras reconstituted with bone marrow and thymus grafts.", "content": "CBA/H and CBA/HT676 radiation chimeras were prepared by lethal irradiation and subsequent reconstitution with bone marrow of the host karyotype and thymocytes of the opposite karyotype. After T- and B-cell chimerism had been established, the animals were inoculated with SEWA or TA3Ha ascites tumor--donor cell hybrids were isolated following the explantation of TA3Ha tumor in selective HAT medium and by selecting for adherent cells from SEWA. The T6T6 chromosomal marker served to distinguish between the type of cell involved in the fusion. In all hybrids the donor component was a nonthymus-derived cell.", "contents": "Tumor-host cell hybrids in radiochimeras reconstituted with bone marrow and thymus grafts. CBA/H and CBA/HT676 radiation chimeras were prepared by lethal irradiation and subsequent reconstitution with bone marrow of the host karyotype and thymocytes of the opposite karyotype. After T- and B-cell chimerism had been established, the animals were inoculated with SEWA or TA3Ha ascites tumor--donor cell hybrids were isolated following the explantation of TA3Ha tumor in selective HAT medium and by selecting for adherent cells from SEWA. The T6T6 chromosomal marker served to distinguish between the type of cell involved in the fusion. In all hybrids the donor component was a nonthymus-derived cell."}
{"id": "PMID:1088469", "title": "Prevention of peritoneal adhesions with aprotinin (trasylol).", "content": "An objective assessment of intraperitoneal trasylol in the prevention of abdominal adhesions in 20 patients has shown that Trasylol resulted in a marked reduction in adhesions in 16 patients who underwent 'second look' procedures. No side-effects were noted and all wounds healed without complications.", "contents": "Prevention of peritoneal adhesions with aprotinin (trasylol). An objective assessment of intraperitoneal trasylol in the prevention of abdominal adhesions in 20 patients has shown that Trasylol resulted in a marked reduction in adhesions in 16 patients who underwent 'second look' procedures. No side-effects were noted and all wounds healed without complications."}
{"id": "PMID:1088467", "title": "[Study of the monoaminergic mechanisms of the effect of haloperidol in experiments using cats].", "content": "Monoaminergic mechanisms of the haloperidol action on the systems \"rewards\" and \"punishments\" were studied in 22 cats with nichrome electrodes implanted into the hypothalamus. Haloperidol was introduced against the background of the dopaminomimetic amantadine, precursors of biogenic amines L-DOPA, tryptophan and 5-OTP. The inhibitory influence of haloperidol on the system \"rewards\" was found to be materialized largely at the expense of its serotonin-negative effect, while in the activating action of haloperidol on the system \"punishment\" its serotonin- and dopamino-negative effects partook.", "contents": "[Study of the monoaminergic mechanisms of the effect of haloperidol in experiments using cats]. Monoaminergic mechanisms of the haloperidol action on the systems \"rewards\" and \"punishments\" were studied in 22 cats with nichrome electrodes implanted into the hypothalamus. Haloperidol was introduced against the background of the dopaminomimetic amantadine, precursors of biogenic amines L-DOPA, tryptophan and 5-OTP. The inhibitory influence of haloperidol on the system \"rewards\" was found to be materialized largely at the expense of its serotonin-negative effect, while in the activating action of haloperidol on the system \"punishment\" its serotonin- and dopamino-negative effects partook."}
{"id": "PMID:1088471", "title": "Cell numbers in human lymphocyte cultures stimulated with pokeweed mitogen.", "content": "As determined by electronic cell counting, the cell numbers in pokeweed mitogen (PWM) stimulated cultures of normal lymphocytes decreased by about 13% during the first day and then remained almost constant up to day 8. In contrast, a progressive decrease of the cell count was observed in cultures of chronic lymphocytic leukaemia (CLL) lymphocytes reaching about 40% of the initial number on day 8. In PWM cultures of normal lymphocytes the transformed cells increased to about 20% of the cells present on day 4, whereas in cultures of CLL lymphocytes these cells reached only 11% on day 7.", "contents": "Cell numbers in human lymphocyte cultures stimulated with pokeweed mitogen. As determined by electronic cell counting, the cell numbers in pokeweed mitogen (PWM) stimulated cultures of normal lymphocytes decreased by about 13% during the first day and then remained almost constant up to day 8. In contrast, a progressive decrease of the cell count was observed in cultures of chronic lymphocytic leukaemia (CLL) lymphocytes reaching about 40% of the initial number on day 8. In PWM cultures of normal lymphocytes the transformed cells increased to about 20% of the cells present on day 4, whereas in cultures of CLL lymphocytes these cells reached only 11% on day 7."}
{"id": "PMID:1088468", "title": "[Morphological characteristics of the internal organs of experimental animals after administration of different doses of chanerol].", "content": "Experiments were conducted  with rats and dogs to study the influence of different hanerol doses on the condition of anumber of internal organs. The drug was introduced in a single dose: to rats in the form of a 0.1% solution in amounts of 6, 8, 10, 13, 20 and 30 mg/kg and to dogs as a 0.1% solution in doses of 24 mg/kg. Doses of 20 and 30 mg/kg for rats and 24 mg/kg for dogs proved intolerable and animals perished in consequence of circulatory disturbances (blood effusion, thrombosis of capillaries in the lungs, spleen, suprarenals and in organs of the gastro-intestinal tract). On application of doses amounting to 6 and 13 mg/kg in rats and 6-12 in dogs the animals remained alive, although the lungs, testes and the spleen were found to be afflicted with thrombosis of individual capillaries and there was demonstrable the the development of pneumosclerotic foci, atrophy of spermatogenic epithelium and slight splenic infarctions.", "contents": "[Morphological characteristics of the internal organs of experimental animals after administration of different doses of chanerol]. Experiments were conducted  with rats and dogs to study the influence of different hanerol doses on the condition of anumber of internal organs. The drug was introduced in a single dose: to rats in the form of a 0.1% solution in amounts of 6, 8, 10, 13, 20 and 30 mg/kg and to dogs as a 0.1% solution in doses of 24 mg/kg. Doses of 20 and 30 mg/kg for rats and 24 mg/kg for dogs proved intolerable and animals perished in consequence of circulatory disturbances (blood effusion, thrombosis of capillaries in the lungs, spleen, suprarenals and in organs of the gastro-intestinal tract). On application of doses amounting to 6 and 13 mg/kg in rats and 6-12 in dogs the animals remained alive, although the lungs, testes and the spleen were found to be afflicted with thrombosis of individual capillaries and there was demonstrable the the development of pneumosclerotic foci, atrophy of spermatogenic epithelium and slight splenic infarctions."}
{"id": "PMID:1088475", "title": "The effect of lesions of the accessory optic tract terminal nuclei on the gonadal response to light in ferrets.", "content": "Electrolytic lesions were made in the lateral (LTN), medial (MTN) or in both terminal nuclei of the accessory optic tract in female ferrets. Either the ipsilateral or contralateral eye was removed. Complete destruction of both nuclei, as assessed by both histological and autoradiographic criteria, did not prevent the acceleration of estrus by artificial long days (L:D, 14:10) in any animal. Although the ferret has no anterior (inferior) accessory tract, lesions were also made in the medial forebrain bundle (MFB) area in ipsilaterally and contralaterally enucleated animals. Estrus still occurred in response to artificial long days in these animals. The experiments suggest that the accessory optic system in the ferret does not transmit information to the pineal regulating the annual breeding season.", "contents": "The effect of lesions of the accessory optic tract terminal nuclei on the gonadal response to light in ferrets. Electrolytic lesions were made in the lateral (LTN), medial (MTN) or in both terminal nuclei of the accessory optic tract in female ferrets. Either the ipsilateral or contralateral eye was removed. Complete destruction of both nuclei, as assessed by both histological and autoradiographic criteria, did not prevent the acceleration of estrus by artificial long days (L:D, 14:10) in any animal. Although the ferret has no anterior (inferior) accessory tract, lesions were also made in the medial forebrain bundle (MFB) area in ipsilaterally and contralaterally enucleated animals. Estrus still occurred in response to artificial long days in these animals. The experiments suggest that the accessory optic system in the ferret does not transmit information to the pineal regulating the annual breeding season."}
{"id": "PMID:1088476", "title": "Normal haemoglobin values in urban Polynesian infants: the possible deleterious influence of artificial feeding.", "content": "A longitudinal survey of urban Polynesian infants in the first year of life provided data on gastro-intestinal bleeding and haemoglobin estimations. The results show that urban Polynesian infants are significantly more anaemic than their European counterparts, and that cow's milk-induced gastro-intestinal bleeding may be a significant cause of this anaemia.", "contents": "Normal haemoglobin values in urban Polynesian infants: the possible deleterious influence of artificial feeding. A longitudinal survey of urban Polynesian infants in the first year of life provided data on gastro-intestinal bleeding and haemoglobin estimations. The results show that urban Polynesian infants are significantly more anaemic than their European counterparts, and that cow's milk-induced gastro-intestinal bleeding may be a significant cause of this anaemia."}
{"id": "PMID:1088477", "title": "Na+ and K+ levels in living cells: do they depend on the rate of outward transport of Na+?", "content": "At 25 degrees C, frog sartorius muslces rapidly gained Na+ and lost K+ in iodoacetamide and pure nitrogen. Beginning at normal levels, the concentrations of these ions in the cells reached those in the surrounding Ringer solution in 140 min. Yet during that time the Na+ efflux rate showed no sign of the slowing down demanded by Na-pump theory. The data support the view that maintenance and alterations of N1+ levels in frog muslce cells reflect adsorption on protein sites and the solubility property of bulk phase water and are independent of the rate at which Na+ leaves the cell surface.", "contents": "Na+ and K+ levels in living cells: do they depend on the rate of outward transport of Na+? At 25 degrees C, frog sartorius muslces rapidly gained Na+ and lost K+ in iodoacetamide and pure nitrogen. Beginning at normal levels, the concentrations of these ions in the cells reached those in the surrounding Ringer solution in 140 min. Yet during that time the Na+ efflux rate showed no sign of the slowing down demanded by Na-pump theory. The data support the view that maintenance and alterations of N1+ levels in frog muslce cells reflect adsorption on protein sites and the solubility property of bulk phase water and are independent of the rate at which Na+ leaves the cell surface."}
{"id": "PMID:1088479", "title": "[Method for detecting the relationship between the initial degree of cellular resistance and its changes under the effect of the stimulants].", "content": "Methods have been elaborated for detecting the individual changes in the resistance of cells on the basis of the analysis of alcohol- and heat resistance of isolated muscles of Rana temporaria L. A correlation has been determined between the initial resistance level of cells and its change induced by experimental effects. This method allows to identify a really existing relation as opposed to the seeming one caused by random factors. The study of this makes it possible to detect the responses which fail to be found by traditional methods of statistical treatment of experimental material.", "contents": "[Method for detecting the relationship between the initial degree of cellular resistance and its changes under the effect of the stimulants]. Methods have been elaborated for detecting the individual changes in the resistance of cells on the basis of the analysis of alcohol- and heat resistance of isolated muscles of Rana temporaria L. A correlation has been determined between the initial resistance level of cells and its change induced by experimental effects. This method allows to identify a really existing relation as opposed to the seeming one caused by random factors. The study of this makes it possible to detect the responses which fail to be found by traditional methods of statistical treatment of experimental material."}
{"id": "PMID:1088480", "title": "[Effect of insulin and adrenaline on the respiration and glycolysis of the skeletal muscle of frog].", "content": "It was shown in the experiments on isolated sartorius muscles of the frog that insulin increased endogenous respiration by 10 mm3 O2 hr per gramm of wet weight. The effect of insulin and epinephrine on respiration is additive. Insulin in middle (5--50 milliunits/ml) and low, physiological, (50 microunits/ml) concentration does not induce acceleration of glycolyses. The conclusion was confirmed that the increase of the endogenous respiration by insulin cannot be accounted for by the mobilization of the substrate of glycolysis. In the presence of epinephrine, the low concentration (50 microunits/ml) of insulin induces the decrease of the content of glucose-6-phosphate. It is probably connected with the influence of insulin on the concentration level of the cAMP.", "contents": "[Effect of insulin and adrenaline on the respiration and glycolysis of the skeletal muscle of frog]. It was shown in the experiments on isolated sartorius muscles of the frog that insulin increased endogenous respiration by 10 mm3 O2 hr per gramm of wet weight. The effect of insulin and epinephrine on respiration is additive. Insulin in middle (5--50 milliunits/ml) and low, physiological, (50 microunits/ml) concentration does not induce acceleration of glycolyses. The conclusion was confirmed that the increase of the endogenous respiration by insulin cannot be accounted for by the mobilization of the substrate of glycolysis. In the presence of epinephrine, the low concentration (50 microunits/ml) of insulin induces the decrease of the content of glucose-6-phosphate. It is probably connected with the influence of insulin on the concentration level of the cAMP."}
{"id": "PMID:1088508", "title": "Development of tumours in Syrian hamsters during prolonged experimental exposure to nas.", "content": "In several series of experiments, nas was introduced into the buccal pouch or oesophagus of Syrian hamsters or applied to the dorsal skin. In one series, nas was administered to six consecutive generations of hamsters. No tumours appeared at the site of administration, although changes appeared in the mucosa of the buccal pouch and the oesophagus. Tumours in organs remote from the site of application developed only in those series in which nas was introduced into the buccal pouch. The commonest site of tumorigenesis was the liver, followed by the adrenal glands, the forestomach, and the genital organs. Tumours occurred in 17.6% of the experimental animals compared with 3.5% of the controls.", "contents": "Development of tumours in Syrian hamsters during prolonged experimental exposure to nas. In several series of experiments, nas was introduced into the buccal pouch or oesophagus of Syrian hamsters or applied to the dorsal skin. In one series, nas was administered to six consecutive generations of hamsters. No tumours appeared at the site of administration, although changes appeared in the mucosa of the buccal pouch and the oesophagus. Tumours in organs remote from the site of application developed only in those series in which nas was introduced into the buccal pouch. The commonest site of tumorigenesis was the liver, followed by the adrenal glands, the forestomach, and the genital organs. Tumours occurred in 17.6% of the experimental animals compared with 3.5% of the controls."}
{"id": "PMID:1088511", "title": "The role of the individual and the community in the research, development, and use of biologicals with criteria for guidelines: a memorandum.", "content": "In view of the widely recognized need to use available vaccines and other biologicals and to develop new ones to control many diseases of world-wide importance, this Memorandum considers the increasingly complex problems that face investigators and public authorities that must review and approve pre-licensing studies and also large-scale regular use. It is stressed that the proper conduct of biologicals research in human beings must be considered from the scientific, sociological, ethical, and legal points of view. The Declaration of Helsinki is regarded of fundamental importance and its applicability to biologicals research is discussed. Recommendations are made for continued international collaboration in this field and \"Criteria on the Role of the Individual and the Community in the Research, Development, and Use of Biologicals\" are formulated. General criteria and specific criteria related to the design of field trials, human involvement in field trials, and surveillance of safety and effectiveness of biologicals in routine use are discussed.", "contents": "The role of the individual and the community in the research, development, and use of biologicals with criteria for guidelines: a memorandum. In view of the widely recognized need to use available vaccines and other biologicals and to develop new ones to control many diseases of world-wide importance, this Memorandum considers the increasingly complex problems that face investigators and public authorities that must review and approve pre-licensing studies and also large-scale regular use. It is stressed that the proper conduct of biologicals research in human beings must be considered from the scientific, sociological, ethical, and legal points of view. The Declaration of Helsinki is regarded of fundamental importance and its applicability to biologicals research is discussed. Recommendations are made for continued international collaboration in this field and \"Criteria on the Role of the Individual and the Community in the Research, Development, and Use of Biologicals\" are formulated. General criteria and specific criteria related to the design of field trials, human involvement in field trials, and surveillance of safety and effectiveness of biologicals in routine use are discussed."}
{"id": "PMID:1088512", "title": "Influenza and other actue respiratory diseases in the Czech Socialist Republic, 1969-1974.", "content": "Since 1969, the incidence of acute respiratory diseases (ARD) in the Czech Socialist Republic of Czechoslovakia has been monitored by a special programme based on reports from 85 district epidemiological centres. In this paper, the incidence of ARD in three age groups, together with the incidence of complications and death rates, are presented for each season during the period 1969-1974. The significance of epidemiological observations and laboratory investigations relating to influenza and other ARD agents, such as parainfluenza viruses, adenoviruses, rhinoviruses, RS virus, coronaviruses, and Mycoplasma pneumoniae, is discussed.", "contents": "Influenza and other actue respiratory diseases in the Czech Socialist Republic, 1969-1974. Since 1969, the incidence of acute respiratory diseases (ARD) in the Czech Socialist Republic of Czechoslovakia has been monitored by a special programme based on reports from 85 district epidemiological centres. In this paper, the incidence of ARD in three age groups, together with the incidence of complications and death rates, are presented for each season during the period 1969-1974. The significance of epidemiological observations and laboratory investigations relating to influenza and other ARD agents, such as parainfluenza viruses, adenoviruses, rhinoviruses, RS virus, coronaviruses, and Mycoplasma pneumoniae, is discussed."}
{"id": "PMID:1088513", "title": "Simple tests for the diagnosis of picornavirus epidemic conjunctivitis (acute haemorrhagic conjunctivitis).", "content": "Simple tests for the study of picornavirus epidemic conjunctivitis are described. The virus was successfully isolated in wells of microtitration plates containing HeLa cell suspension and the isolates were easily identifiable by neutralization in the micrometabolic inhibition test. For the estimation of antibody titre in patients' sera, the latter procedure was found to be as reliable as neutralization in tissue culture tubes. These micromethods would enable virus laboratories not equipped for tissue culture work to study this new ocular disease, which has been endemic in a number of countries since the pandemic outbreak of 1969-72 in Africa, Asia, and Europe (London).", "contents": "Simple tests for the diagnosis of picornavirus epidemic conjunctivitis (acute haemorrhagic conjunctivitis). Simple tests for the study of picornavirus epidemic conjunctivitis are described. The virus was successfully isolated in wells of microtitration plates containing HeLa cell suspension and the isolates were easily identifiable by neutralization in the micrometabolic inhibition test. For the estimation of antibody titre in patients' sera, the latter procedure was found to be as reliable as neutralization in tissue culture tubes. These micromethods would enable virus laboratories not equipped for tissue culture work to study this new ocular disease, which has been endemic in a number of countries since the pandemic outbreak of 1969-72 in Africa, Asia, and Europe (London)."}
{"id": "PMID:1088514", "title": "Booster effect of Sabin poliomyelitis vaccine.", "content": "Studies were carried out on 86 children aged 1-14 years who had titres of antibodies against poliovirus type 3 lower than 1: 32 and against type 2 higher than 1: 4. After a booster dose of poliomyelitis vaccine type 2+3, seroconversion took place in 44 (90%) of 49 children whose original titre was lower than 1: 4 and the geometrical mean titre increased to 1: 244. Of 37 children with low original titres of antibodies against type 3, i.e., 1: 4, 1: 8, or 1: 16, the titre increased fourfold or more in 31 (84%) and the geometrical mean titre rose to 1: 175. In 11 children (13%), no significant increase in titre took place.", "contents": "Booster effect of Sabin poliomyelitis vaccine. Studies were carried out on 86 children aged 1-14 years who had titres of antibodies against poliovirus type 3 lower than 1: 32 and against type 2 higher than 1: 4. After a booster dose of poliomyelitis vaccine type 2+3, seroconversion took place in 44 (90%) of 49 children whose original titre was lower than 1: 4 and the geometrical mean titre increased to 1: 244. Of 37 children with low original titres of antibodies against type 3, i.e., 1: 4, 1: 8, or 1: 16, the titre increased fourfold or more in 31 (84%) and the geometrical mean titre rose to 1: 175. In 11 children (13%), no significant increase in titre took place."}
{"id": "PMID:1088516", "title": "A simple thick-smear technique for the diagnosis of Schistosoma mansoni infection.", "content": "A smear technique is described in which thick glass coverslips are substituted for cellulose acetate film soaked in glycerine as used in the Kato technique for stool examination. Estimates of the sensitivity and applicability of this technique for Schistosoma mansoni infections indicate that it compares favourably with the Kato technique, especially for field use.", "contents": "A simple thick-smear technique for the diagnosis of Schistosoma mansoni infection. A smear technique is described in which thick glass coverslips are substituted for cellulose acetate film soaked in glycerine as used in the Kato technique for stool examination. Estimates of the sensitivity and applicability of this technique for Schistosoma mansoni infections indicate that it compares favourably with the Kato technique, especially for field use."}
{"id": "PMID:1088517", "title": "Variations in infectivity for Biomphalaria glabrata in strains of Schistosoma mansoni from the same geographical area.", "content": "Genetic differences in infectivity for intermediate snail hosts in two strains of S. mansoni from the same geographical area and within a single isolated parasite population are reported. The importance of recognizing the potential for such genetic variation is stressed.", "contents": "Variations in infectivity for Biomphalaria glabrata in strains of Schistosoma mansoni from the same geographical area. Genetic differences in infectivity for intermediate snail hosts in two strains of S. mansoni from the same geographical area and within a single isolated parasite population are reported. The importance of recognizing the potential for such genetic variation is stressed."}
{"id": "PMID:1088518", "title": "Transmission in utero of microfilariae of Onchocerca volvulus.", "content": "Skin snips from newborn children and biopsy material from the umbilical cord and placenta of their mothers were examined. Microfilariae of O. volvulus were found in the skin of 2 out of 11 babies and in the umbilical cords of 2 out of 11 babies born to ten mothers infected with O. volvulus in Bawku Hospital, Ghana.", "contents": "Transmission in utero of microfilariae of Onchocerca volvulus. Skin snips from newborn children and biopsy material from the umbilical cord and placenta of their mothers were examined. Microfilariae of O. volvulus were found in the skin of 2 out of 11 babies and in the umbilical cords of 2 out of 11 babies born to ten mothers infected with O. volvulus in Bawku Hospital, Ghana."}
{"id": "PMID:1088523", "title": "Effect of malnutrition on several parameters of the immune system of children.", "content": "In order to study the relationship between nutritional status and immunity, certain biochemical and immunological parameters were examined in 53 children with mild or severe malnutrition and 35 normal controls. The levels of hemoglobin, total serum protein and complement (C'3) were not affected by malnutrition. There was a significant depression of serum albumin, transferrin and ceruloplasmin in the severely malnourished children. Serum IgM, IgG and IgD were normal in both malnourished groups. The level of serum IgA was elevated only in the severely malnourished children. The proportion of B cells was the same in all groups; howver, the frequency of T cells was reduced in the severely malnourished cases.", "contents": "Effect of malnutrition on several parameters of the immune system of children. In order to study the relationship between nutritional status and immunity, certain biochemical and immunological parameters were examined in 53 children with mild or severe malnutrition and 35 normal controls. The levels of hemoglobin, total serum protein and complement (C'3) were not affected by malnutrition. There was a significant depression of serum albumin, transferrin and ceruloplasmin in the severely malnourished children. Serum IgM, IgG and IgD were normal in both malnourished groups. The level of serum IgA was elevated only in the severely malnourished children. The proportion of B cells was the same in all groups; howver, the frequency of T cells was reduced in the severely malnourished cases."}
{"id": "PMID:1088538", "title": "Pathologic nystagmus: a classification based on electro-oculographic recordings.", "content": "The widespread availability of eye movement recording systems has added a new dimension to our ability to accurately describe nystagmus. Electrooculography (EOG) is the simplest and most readily available system. With EOG the effect of loss of fixation (either with eyes closed, or eyes open in darkness) can be accurately assessed and quantitative evaluation of nystagmus speed, frequency and amplitude is easily made. Of equal importance, it provides a permanent record that can be compared with nystagmus recorded in other patients. By comparing clinical observation with paper recordings, both students and experienced clinicians can become more efficient in recognizing different varieties of nystagmus.", "contents": "Pathologic nystagmus: a classification based on electro-oculographic recordings. The widespread availability of eye movement recording systems has added a new dimension to our ability to accurately describe nystagmus. Electrooculography (EOG) is the simplest and most readily available system. With EOG the effect of loss of fixation (either with eyes closed, or eyes open in darkness) can be accurately assessed and quantitative evaluation of nystagmus speed, frequency and amplitude is easily made. Of equal importance, it provides a permanent record that can be compared with nystagmus recorded in other patients. By comparing clinical observation with paper recordings, both students and experienced clinicians can become more efficient in recognizing different varieties of nystagmus."}
{"id": "PMID:1088542", "title": "[Conformations and interatomic distances in polymorphs of sulfanilamide].", "content": "By CNDO (Complete Neglect of Differential Overlap) molecular orbital method, interatomic distances and XYZ cartesian corrdinates were calculated in five polymorphs (monohydrated, alpha, two beta, and gamma) of sulfanilamide. Interatomic distances thus obtained are very close to those originally presented by Bells & Roblin and support the mechanism of action postulated long algo for sulfa drugs as being competitive antagonism with p-aminobenzoic acid.", "contents": "[Conformations and interatomic distances in polymorphs of sulfanilamide]. By CNDO (Complete Neglect of Differential Overlap) molecular orbital method, interatomic distances and XYZ cartesian corrdinates were calculated in five polymorphs (monohydrated, alpha, two beta, and gamma) of sulfanilamide. Interatomic distances thus obtained are very close to those originally presented by Bells & Roblin and support the mechanism of action postulated long algo for sulfa drugs as being competitive antagonism with p-aminobenzoic acid."}
{"id": "PMID:1088553", "title": "The effect of lithium chloride on morphine- and pyrogen-induced hyperthermia in rats.", "content": "In rats, doses of morphine between 2.5 and 20 mg/kg produced hyperthermia, 50-400 mg/kg lithium chloride induced dose-dependent hypothermia. These doses of lithium chloride inhibited morphine- and pyrogen-induced hyperthermia. The hypothermic effect of lithium may be due to the replacement of sodium ions by lithium ions in the hypothalamus. The antagonism between morphine and lithium is nonspecific.", "contents": "The effect of lithium chloride on morphine- and pyrogen-induced hyperthermia in rats. In rats, doses of morphine between 2.5 and 20 mg/kg produced hyperthermia, 50-400 mg/kg lithium chloride induced dose-dependent hypothermia. These doses of lithium chloride inhibited morphine- and pyrogen-induced hyperthermia. The hypothermic effect of lithium may be due to the replacement of sodium ions by lithium ions in the hypothalamus. The antagonism between morphine and lithium is nonspecific."}
{"id": "PMID:1088555", "title": "Hearing in patients with congenital deformity of the inner ear.", "content": "Congenital abnormalities of the bony labyrinth and internal auditory meatus range from complete absence to minor anomalies compatible with normal cochlea function. A 'follow-up' survey to assess the hearing of 56 patients with abnormalities of the inner ear shown by tomography was made. The hearing levels are discussed in relation to the tomographic appearances and comparison made with other series of inner ear abnormalities demonstrated both radiographically and histologically.", "contents": "Hearing in patients with congenital deformity of the inner ear. Congenital abnormalities of the bony labyrinth and internal auditory meatus range from complete absence to minor anomalies compatible with normal cochlea function. A 'follow-up' survey to assess the hearing of 56 patients with abnormalities of the inner ear shown by tomography was made. The hearing levels are discussed in relation to the tomographic appearances and comparison made with other series of inner ear abnormalities demonstrated both radiographically and histologically."}
{"id": "PMID:1088559", "title": "[The influence of antimetabolites on Trichomonas vaginalis as an experimental model].", "content": "The present study was concerned with the relationship between antimetabolites and Trichomonas vaginalis. In all of the strains (with p-nitrobenzylether of N-acetyldiiodtryrosine) tested, the growth of Trichomonas vaginalis was identical to that of the control series at concentrations up to 2 mg/ml. At higher concentrations a decrease in multiplication was noted. In the experimental series with antitrypsin, from 1 mg/ml produced an inhibiting effect upon the Trichomonas population. Further investigations on the combined inhibition test are envisaged.", "contents": "[The influence of antimetabolites on Trichomonas vaginalis as an experimental model]. The present study was concerned with the relationship between antimetabolites and Trichomonas vaginalis. In all of the strains (with p-nitrobenzylether of N-acetyldiiodtryrosine) tested, the growth of Trichomonas vaginalis was identical to that of the control series at concentrations up to 2 mg/ml. At higher concentrations a decrease in multiplication was noted. In the experimental series with antitrypsin, from 1 mg/ml produced an inhibiting effect upon the Trichomonas population. Further investigations on the combined inhibition test are envisaged."}
{"id": "PMID:1088564", "title": "[Effect of Solcoseryl on nerve tissue under in vitro conditions].", "content": "Explants from trigeminal ganglia and skin of chick embryos and hippocampus from fetal rats were cultivated in Maximow assembly in the presence of Solcoseryl (Solco AG, Basel), a blood extract of calf. Solcoseryl in vitro did not influence the regeneration of nerve fibers from CNS explants. A stimulatory effect of Solcoseryl in vitro by 1% concentration on the outgrowth of new processes in explants of PNS was demonstrated. It is discussed: under optimal concentration Solcoseryl may be important for the influence of the composition of the medium in which explants of the nerve system and skin are cultivated.", "contents": "[Effect of Solcoseryl on nerve tissue under in vitro conditions]. Explants from trigeminal ganglia and skin of chick embryos and hippocampus from fetal rats were cultivated in Maximow assembly in the presence of Solcoseryl (Solco AG, Basel), a blood extract of calf. Solcoseryl in vitro did not influence the regeneration of nerve fibers from CNS explants. A stimulatory effect of Solcoseryl in vitro by 1% concentration on the outgrowth of new processes in explants of PNS was demonstrated. It is discussed: under optimal concentration Solcoseryl may be important for the influence of the composition of the medium in which explants of the nerve system and skin are cultivated."}
{"id": "PMID:1088565", "title": "[Effect of orotic acid on the in vitro cultured nerve tissue].", "content": "Cell- and explantcultures of the hippocampus of 18 days old fetal rats and explantcultures of the ganglion trigeminal of 9 days old chick embryos were cultivated in Maximow-chambers. Orotic acid, Sodiumorotate and methylglucaminorotate by concentrations of 10(-3)...10(-7) g/ml medium were added to the cultures. By concentrations of 10(-7) g/ml medium in the CNS-cultures and 10(-6) g/ml medium in the PNS-cultures is stimulated the nerve fiber growth and is increased the nerve fiber index. After three weeks in vitro is a higher number of neurons in the cultures compared to the controls.", "contents": "[Effect of orotic acid on the in vitro cultured nerve tissue]. Cell- and explantcultures of the hippocampus of 18 days old fetal rats and explantcultures of the ganglion trigeminal of 9 days old chick embryos were cultivated in Maximow-chambers. Orotic acid, Sodiumorotate and methylglucaminorotate by concentrations of 10(-3)...10(-7) g/ml medium were added to the cultures. By concentrations of 10(-7) g/ml medium in the CNS-cultures and 10(-6) g/ml medium in the PNS-cultures is stimulated the nerve fiber growth and is increased the nerve fiber index. After three weeks in vitro is a higher number of neurons in the cultures compared to the controls."}
{"id": "PMID:1088566", "title": "[Morphometric studies of prepyramidal and pyramidal neurons of various vertebrates].", "content": "Praepyramidal and pyramidal neurons were demonstrated in homologous telencephalic areas of Salmo irideus (Gibbons 1855), Rana temporaria L., Rattus norvegicus, forma alba, by means of the Golgi-technique. A comparative morphometrical analysis was made concerning the following parameters: main dendritic length, length of the pericaryon, number of dendritic spines in the first 50-micrometer-dendritic segment and length of the spines-free zone. From these data a quotient was calculated and expressing the theoretical dendritic length per ten spines. There is an increase of length of the pericaryon, length of the spines-free zone, main dendritic length and a pseudo-decrease of the number of dendritic spines in the first 50 micrometer-dendritic segment in rats, as compared with Rana and Salmo. The results are discussed with respect to following problems: homology, phylogenesis and corticalisation.", "contents": "[Morphometric studies of prepyramidal and pyramidal neurons of various vertebrates]. Praepyramidal and pyramidal neurons were demonstrated in homologous telencephalic areas of Salmo irideus (Gibbons 1855), Rana temporaria L., Rattus norvegicus, forma alba, by means of the Golgi-technique. A comparative morphometrical analysis was made concerning the following parameters: main dendritic length, length of the pericaryon, number of dendritic spines in the first 50-micrometer-dendritic segment and length of the spines-free zone. From these data a quotient was calculated and expressing the theoretical dendritic length per ten spines. There is an increase of length of the pericaryon, length of the spines-free zone, main dendritic length and a pseudo-decrease of the number of dendritic spines in the first 50 micrometer-dendritic segment in rats, as compared with Rana and Salmo. The results are discussed with respect to following problems: homology, phylogenesis and corticalisation."}
{"id": "PMID:1088567", "title": "Action of ethanol and other solvents on the nerve tissue under in vitro conditions.", "content": "Explants and cells of nervous tissue were cultivated in the presence of aethanol, tween 80, dimethylformamid (DMF) and dimethylsulfoxid (DMSO) and the influence upon the index of area, the growth rate and fiber index was observed. They are important to solutions of drugs for tests in vitro. At the beginning of the cultivation aethanol in vitro influenced the regeneration of nerve fibers from explants and cells. A significant increase of the index of growth was observed. After a long term influence of tween 80, DMF and DMSO an inhibition of differentiation of neurons in vitro was observed.", "contents": "Action of ethanol and other solvents on the nerve tissue under in vitro conditions. Explants and cells of nervous tissue were cultivated in the presence of aethanol, tween 80, dimethylformamid (DMF) and dimethylsulfoxid (DMSO) and the influence upon the index of area, the growth rate and fiber index was observed. They are important to solutions of drugs for tests in vitro. At the beginning of the cultivation aethanol in vitro influenced the regeneration of nerve fibers from explants and cells. A significant increase of the index of growth was observed. After a long term influence of tween 80, DMF and DMSO an inhibition of differentiation of neurons in vitro was observed."}
{"id": "PMID:1088569", "title": "[X-ray cinematographic studies of local ventricular dynamics after aortocoronary bypass].", "content": "In 12 patients with high-grade coronary stenosis local motility before and after aortocoronary vein bypass was estimated by cineradiography of coronary bifurcations and surgically implanted myocardial metal markers. A group of patients with relief of angina showed significant increase of local segment shortening within the first postoperative days. During the following months no further alteration occurred. In three patients with persisting angina no improvement of local motility could be found.", "contents": "[X-ray cinematographic studies of local ventricular dynamics after aortocoronary bypass]. In 12 patients with high-grade coronary stenosis local motility before and after aortocoronary vein bypass was estimated by cineradiography of coronary bifurcations and surgically implanted myocardial metal markers. A group of patients with relief of angina showed significant increase of local segment shortening within the first postoperative days. During the following months no further alteration occurred. In three patients with persisting angina no improvement of local motility could be found."}
{"id": "PMID:1088570", "title": "Coronary and contractile reserve in the dog heart with chronic multiple coronary occlusions before and after aortocoronary bypass.", "content": "Peak isovolumetric left ventricular pressure and dp/dtmax before and after norepinephrine (N.E.) infusion and cross-clamping of the aorta was identical in normal dogs and in dogs with chronic coronary artery occlusion (C.C.O.) before and after aortocoronary bypass. In normal dogs, coronary reserve was 7.9 and 7.4 times control for the subendocardium and the subepicardium. After C.C.O., coronary reserve was 7.0 and 5.7 times control in the normal area and 2.4 and 3.5 times control in the collateral dependent area (endo vs. epi). After N.E. myocardial blood flow increased to 57% of the coronary reserve in normal dogs, and to 100% in dogs with C.C.O. After bypass, myocardial blood flow normalized. In dogs with C.C.O., N.E. stimulated the contractile reserve maximally and the coronary reserve is completely expended.", "contents": "Coronary and contractile reserve in the dog heart with chronic multiple coronary occlusions before and after aortocoronary bypass. Peak isovolumetric left ventricular pressure and dp/dtmax before and after norepinephrine (N.E.) infusion and cross-clamping of the aorta was identical in normal dogs and in dogs with chronic coronary artery occlusion (C.C.O.) before and after aortocoronary bypass. In normal dogs, coronary reserve was 7.9 and 7.4 times control for the subendocardium and the subepicardium. After C.C.O., coronary reserve was 7.0 and 5.7 times control in the normal area and 2.4 and 3.5 times control in the collateral dependent area (endo vs. epi). After N.E. myocardial blood flow increased to 57% of the coronary reserve in normal dogs, and to 100% in dogs with C.C.O. After bypass, myocardial blood flow normalized. In dogs with C.C.O., N.E. stimulated the contractile reserve maximally and the coronary reserve is completely expended."}
{"id": "PMID:1088571", "title": "[Stimulation and differentiation of lymphocytes after injuries].", "content": "In seven patients who underwent major trauma, stimulation of lymphocytes with phytohaemagglutinin, pokeweed mitogen, and concanavalin A was investigated. Furthermore the differentiation of lymphocytes into T- and B-cells was studied. The synthesis rates of DNA (3H-thymidine incorporation) and RNA (3H-uridine incorporation) are qualitatively depressed at different time intervals. The T- and B-cell relation is altered in favor of the B-lymphocytes.", "contents": "[Stimulation and differentiation of lymphocytes after injuries]. In seven patients who underwent major trauma, stimulation of lymphocytes with phytohaemagglutinin, pokeweed mitogen, and concanavalin A was investigated. Furthermore the differentiation of lymphocytes into T- and B-cells was studied. The synthesis rates of DNA (3H-thymidine incorporation) and RNA (3H-uridine incorporation) are qualitatively depressed at different time intervals. The T- and B-cell relation is altered in favor of the B-lymphocytes."}
{"id": "PMID:1088581", "title": "Tumor immunology. A neurosurgical perspective. I. General concepts of tumor immunology.", "content": "In the quest for the elusive cure of human neoplasms, considerable effort has been expended over the course of the past 10 years to define the immune system as it relates to the development and evolution of cancer in man. At this time thera is ample evidence for, but incomplete of, the existence of a multifactorial immunosurveillance system that is important in the production and control of neoplasms. The framework for this system has been defined and its modification, with the development and progression of various neoplasms, has been studied with great detail. These studies have been undertaken with the hope of harnessing immune mechanisms and altering their response in favor of the host's defenses related to the evolving tumor. This paper will review the major principles and concepts that have evolved.", "contents": "Tumor immunology. A neurosurgical perspective. I. General concepts of tumor immunology. In the quest for the elusive cure of human neoplasms, considerable effort has been expended over the course of the past 10 years to define the immune system as it relates to the development and evolution of cancer in man. At this time thera is ample evidence for, but incomplete of, the existence of a multifactorial immunosurveillance system that is important in the production and control of neoplasms. The framework for this system has been defined and its modification, with the development and progression of various neoplasms, has been studied with great detail. These studies have been undertaken with the hope of harnessing immune mechanisms and altering their response in favor of the host's defenses related to the evolving tumor. This paper will review the major principles and concepts that have evolved."}
{"id": "PMID:1088575", "title": "Sodium-calcium exchange in regulation of cardiac contraction.", "content": "The origin and possible regulatory mechanism of tonic tension (ICa-independent component of active contractile activity) were investigated in frog antrial muscle under voltage-clamp conditions. Replacement of NaCl by LiCl resulted in a fast decrease in tonic tension; a similar fast decrease of this contractile component was induced by Ca-free solution. When low Na Ringer's solution was applied, tonic tension increased transiently and then decreased to a steady amplitude; at return to normal Ringer's, a further, substantial decrease in tonic tension occurred before the original level was reached. Similar behavior of tonic tension was observed when both [Na] o and [Ca]o were lowered, but the ratio [Ca]o/[Na]o2 remained constant; the transient changes were prevented by using low Ca and Na solutions and keeping the ratio of [Ca]o/[Na]o5 constant. The significance of Na-Ca exchange in regulating tonic tension and the possibility that this exchange may be electrogenic are discussed.", "contents": "Sodium-calcium exchange in regulation of cardiac contraction. The origin and possible regulatory mechanism of tonic tension (ICa-independent component of active contractile activity) were investigated in frog antrial muscle under voltage-clamp conditions. Replacement of NaCl by LiCl resulted in a fast decrease in tonic tension; a similar fast decrease of this contractile component was induced by Ca-free solution. When low Na Ringer's solution was applied, tonic tension increased transiently and then decreased to a steady amplitude; at return to normal Ringer's, a further, substantial decrease in tonic tension occurred before the original level was reached. Similar behavior of tonic tension was observed when both [Na] o and [Ca]o were lowered, but the ratio [Ca]o/[Na]o2 remained constant; the transient changes were prevented by using low Ca and Na solutions and keeping the ratio of [Ca]o/[Na]o5 constant. The significance of Na-Ca exchange in regulating tonic tension and the possibility that this exchange may be electrogenic are discussed."}
{"id": "PMID:1088576", "title": "Relaxation of frog myocardium.", "content": "Tension fall of frog heart contraction was analyzed under voltage-clamp conditions. It appears mostly exponential. The rate of relaxation depends upon the extracellular and intracellular Na concentrations. This suggests that the relaxation is under the control of Na-Ca exchange. The speeding up of relaxation by adrenaline in frog heart is revealed by low Na solution, while it is hidden by the primordial Na-Ca exchange in Ringer's solution.", "contents": "Relaxation of frog myocardium. Tension fall of frog heart contraction was analyzed under voltage-clamp conditions. It appears mostly exponential. The rate of relaxation depends upon the extracellular and intracellular Na concentrations. This suggests that the relaxation is under the control of Na-Ca exchange. The speeding up of relaxation by adrenaline in frog heart is revealed by low Na solution, while it is hidden by the primordial Na-Ca exchange in Ringer's solution."}
{"id": "PMID:1088578", "title": "Dual effects of fluoride on the action potential, contraction, and membrane currents in frog atrial muscle.", "content": "The effects of NaF (10(-7) - 10(-2) M) on the membrane potential, current, and contractile tension in bullfrog atrial muscle were investigated under voltage-clamped or unclamped conditions. NaF showed two opposite actions, positive and negative inotropic effects, depending on the concentration of the drug. NaF in lower concentrations produced a marked increase in contractility, a moderate prolongation of action potential, an augmentation of calcium response, and an increase of ICa-dependent tension. Contrarily, NaF in higher concentrations produced an inhibition of contractile tension associated with a shortening of action potential, an increase in potassium conductance, and an inhibition of calcium response. These results indicate that NaF affects the contractile response by dual mechanisms.", "contents": "Dual effects of fluoride on the action potential, contraction, and membrane currents in frog atrial muscle. The effects of NaF (10(-7) - 10(-2) M) on the membrane potential, current, and contractile tension in bullfrog atrial muscle were investigated under voltage-clamped or unclamped conditions. NaF showed two opposite actions, positive and negative inotropic effects, depending on the concentration of the drug. NaF in lower concentrations produced a marked increase in contractility, a moderate prolongation of action potential, an augmentation of calcium response, and an increase of ICa-dependent tension. Contrarily, NaF in higher concentrations produced an inhibition of contractile tension associated with a shortening of action potential, an increase in potassium conductance, and an inhibition of calcium response. These results indicate that NaF affects the contractile response by dual mechanisms."}
{"id": "PMID:1088590", "title": "Inhibition of spontaneous E-rosetting by antithymocyte globulin in Graves disease.", "content": "The number of thymus-dependent (T) lymphocytes and the concentrations of antithymocyte-globulin causing 25% inhibition of E-rosetting were determined in 40 cases of Graves disease. No significant difference in T-lymphocyte count was found between the test groups and the control group. An increased resistance of T-lymphocytes to antithymocyte globulin was found in Graves disease, particularly in its form marked by progressive ophthalmopathy. The increase in resistance was significant in the first five years of the disease.", "contents": "Inhibition of spontaneous E-rosetting by antithymocyte globulin in Graves disease. The number of thymus-dependent (T) lymphocytes and the concentrations of antithymocyte-globulin causing 25% inhibition of E-rosetting were determined in 40 cases of Graves disease. No significant difference in T-lymphocyte count was found between the test groups and the control group. An increased resistance of T-lymphocytes to antithymocyte globulin was found in Graves disease, particularly in its form marked by progressive ophthalmopathy. The increase in resistance was significant in the first five years of the disease."}
{"id": "PMID:1088592", "title": "Determination of OH 5 tryptophan by chromato-spectrofluorimetry in carcinoid tumors.", "content": "We have suggested the determination of different indolic metabolites of tryptophan: OH 5 trptophan (5 HTP), OH 5 tryptamine (5 HT), OH 5 indolyl-acetic acid (5 HIA) by column chromatography on cationic coupled with spectrofluorimetry after amplification obtained with o-phtaldehyde. The different metabolites are determined alone or simultaneously depending on the buffer used. The following qualitates are examined: sensitivity, specificity, accuracy. We have established normal reference values. For 5 HTP the normal values in urine are less than or equal to 50 microgram per day. In 12 cases of carcinoid tumors we have measured 5 HIA, 5 HTP, 5 HT and calculated the ratio between 5 HT and 5 HTP in the urine which makes it possible to have an idea of the enzyme activity in the hydroxyindol pathway in cases of intestinal and bronkial carcinoids.", "contents": "Determination of OH 5 tryptophan by chromato-spectrofluorimetry in carcinoid tumors. We have suggested the determination of different indolic metabolites of tryptophan: OH 5 trptophan (5 HTP), OH 5 tryptamine (5 HT), OH 5 indolyl-acetic acid (5 HIA) by column chromatography on cationic coupled with spectrofluorimetry after amplification obtained with o-phtaldehyde. The different metabolites are determined alone or simultaneously depending on the buffer used. The following qualitates are examined: sensitivity, specificity, accuracy. We have established normal reference values. For 5 HTP the normal values in urine are less than or equal to 50 microgram per day. In 12 cases of carcinoid tumors we have measured 5 HIA, 5 HTP, 5 HT and calculated the ratio between 5 HT and 5 HTP in the urine which makes it possible to have an idea of the enzyme activity in the hydroxyindol pathway in cases of intestinal and bronkial carcinoids."}
{"id": "PMID:1088593", "title": "Enzymic studies on the metabolism of 5-hydroxyindoles in the brain.", "content": "It was shown that rat brain extract catalysed the formation of 5HIAA from 5HTP via a transamination pathway which does not contain 5HT but 5-hydroxyindolepyruvic acid as an intermediate.", "contents": "Enzymic studies on the metabolism of 5-hydroxyindoles in the brain. It was shown that rat brain extract catalysed the formation of 5HIAA from 5HTP via a transamination pathway which does not contain 5HT but 5-hydroxyindolepyruvic acid as an intermediate."}
{"id": "PMID:1088604", "title": "Solid-phase immunoradiometric assay of factor-VIII protein.", "content": "A solid phase immunoradiometric assay for factor-VIII protein has been developed using 125I-labelled rabbit antibody made against highly purified factor VIII. Antibody specific for high molecular weight factor-VIII protein was isolated using an immunoadsorbent consisting of highly purified factor VIII bound to diazotized m-aminobenzyl (oxymethyl)-cellulose. The purified anti-factor VIII antibody was labelled with 125I while bound to the immunoadsorbent and then eluted at pH 2.9. Dilutions of plasma samples for assay were incubated for 48 h in anti-factor VIII antibody-coated tubes. The tubes were washed to remove unbound proteins and the 125I-labelled, purified antibody was added. After 48 h incubation the tubes were washed to remove unbound antibody and counted. The concentration of immunoreactive factor-VIII protein in pooled normal human plasma was determined to be 8 microgram/ml. The minimum amount of factor VIII measured by the assay is less than 0.16 ng. Factor-VIII protein was present in normal concentration in haemophilia A plasma, and in reduced concentration in von Willebrand's disease plasma. The method has the advantages of improved sensitivity, specificity, efficiency and economy as compared with previous factor-VIII immunoassays.", "contents": "Solid-phase immunoradiometric assay of factor-VIII protein. A solid phase immunoradiometric assay for factor-VIII protein has been developed using 125I-labelled rabbit antibody made against highly purified factor VIII. Antibody specific for high molecular weight factor-VIII protein was isolated using an immunoadsorbent consisting of highly purified factor VIII bound to diazotized m-aminobenzyl (oxymethyl)-cellulose. The purified anti-factor VIII antibody was labelled with 125I while bound to the immunoadsorbent and then eluted at pH 2.9. Dilutions of plasma samples for assay were incubated for 48 h in anti-factor VIII antibody-coated tubes. The tubes were washed to remove unbound proteins and the 125I-labelled, purified antibody was added. After 48 h incubation the tubes were washed to remove unbound antibody and counted. The concentration of immunoreactive factor-VIII protein in pooled normal human plasma was determined to be 8 microgram/ml. The minimum amount of factor VIII measured by the assay is less than 0.16 ng. Factor-VIII protein was present in normal concentration in haemophilia A plasma, and in reduced concentration in von Willebrand's disease plasma. The method has the advantages of improved sensitivity, specificity, efficiency and economy as compared with previous factor-VIII immunoassays."}
{"id": "PMID:1088602", "title": "Urethane modification of EEG-like activity and acoustically evoked field potentials recorded from deep nuclei.", "content": "Average acoustic-evoked responses (AAER) and EEGs were recorded from the lateral hypothalamus (LH), ventromedial hypothalamus (VMH), medial forebrain bundle (MFB) and reticular formation (RF) of freely behaving rats before and after various doses (0.2, 0.4, 0.8, 1.2 g/5g) of the anesthetic drug, urethane. The effect of a surgical level of urethane (1.2 g/5g) over time (8--14 h) was also studied. Urethane produced a dose-dependent slowing in the EEG frequency. The effect of 1.2 g/kg over time was a bimodal depression of the EEG frequency. Three components of the AAER were recorded consistently and evaluated in terms of amplitude. With increasing doses, the early component (P2) was depressed in the LH2, VMH and MFB, but not in the RF which remained at control values or higher. The later components (N2 and P2) were depressed in all four structures. This suggests that urethane may be blocking the integrative function of the RF, as well as in the thalamus, resulting in lower sensory input to higher CNS structures.", "contents": "Urethane modification of EEG-like activity and acoustically evoked field potentials recorded from deep nuclei. Average acoustic-evoked responses (AAER) and EEGs were recorded from the lateral hypothalamus (LH), ventromedial hypothalamus (VMH), medial forebrain bundle (MFB) and reticular formation (RF) of freely behaving rats before and after various doses (0.2, 0.4, 0.8, 1.2 g/5g) of the anesthetic drug, urethane. The effect of a surgical level of urethane (1.2 g/5g) over time (8--14 h) was also studied. Urethane produced a dose-dependent slowing in the EEG frequency. The effect of 1.2 g/kg over time was a bimodal depression of the EEG frequency. Three components of the AAER were recorded consistently and evaluated in terms of amplitude. With increasing doses, the early component (P2) was depressed in the LH2, VMH and MFB, but not in the RF which remained at control values or higher. The later components (N2 and P2) were depressed in all four structures. This suggests that urethane may be blocking the integrative function of the RF, as well as in the thalamus, resulting in lower sensory input to higher CNS structures."}
{"id": "PMID:1088603", "title": "Technique for radiofrequency gasserian ganglionectomy.", "content": "Indications, technical procedures, and complications are discussed for the performance of thermal electrocoagulation of the trigeminal preganglionic rootlets and gasserian ganglion. Specific steps in preoperative evaluation, premedication, anesthetic management, and technical performance of graded lesion-making utilizing radiographic control, physiologic stimulation and intraoperative patient monitoring are discussed. A survey of complications is presented.", "contents": "Technique for radiofrequency gasserian ganglionectomy. Indications, technical procedures, and complications are discussed for the performance of thermal electrocoagulation of the trigeminal preganglionic rootlets and gasserian ganglion. Specific steps in preoperative evaluation, premedication, anesthetic management, and technical performance of graded lesion-making utilizing radiographic control, physiologic stimulation and intraoperative patient monitoring are discussed. A survey of complications is presented."}
{"id": "PMID:1088607", "title": "Innovations in health care delivery for patients with end-stage renal disease (ESRD).", "content": "Increasing requirements for dialysis treatment, financial restraints, quality control and the need to increase the efficiency of professionals involved in the delivery and supervision of chronic dialysis services have stimulated the development of innovative techniques in dialysis health care delivery. The Regional Kidney Disease Program, located at Hennepin County Medical Center, has pioneered the development and application of several innovations to achieve more effective regional delivery of dialysis therapy. Three of the major innovations we have developed are described. They include a computerized automated medical information system, an unique multimedia modified programmed learning training program for allied health personnel and a dialysis nurse practitioner program. Several of these innovations have potential application as models for other developments in modern health care delivery.", "contents": "Innovations in health care delivery for patients with end-stage renal disease (ESRD). Increasing requirements for dialysis treatment, financial restraints, quality control and the need to increase the efficiency of professionals involved in the delivery and supervision of chronic dialysis services have stimulated the development of innovative techniques in dialysis health care delivery. The Regional Kidney Disease Program, located at Hennepin County Medical Center, has pioneered the development and application of several innovations to achieve more effective regional delivery of dialysis therapy. Three of the major innovations we have developed are described. They include a computerized automated medical information system, an unique multimedia modified programmed learning training program for allied health personnel and a dialysis nurse practitioner program. Several of these innovations have potential application as models for other developments in modern health care delivery."}
{"id": "PMID:1088608", "title": "Effect of cholinesterase inhibitors on synaptic potentials of the frog neuromuscular junction.", "content": "The action of neostigmine and armin on the parameters of synaptic potentials of the m. cutaneus pectoris of the frog was investigated. Both drugs caused an approximately equal increase in amplitude of miniature end-plate potentials (MEPP) and end-plate potentials (EPP), and lengthened the rise time and the half-decay time of EPP, but the effect of armin developed faster. The presynaptic action of both inhibitors was demonstrated (an increase in the frequency of MEPP and in the quantum composition of EPP), but it developed much more slowly than the postsynaptic effects; it was more marked in the case of neostigmine. Administration of armin after preliminary contact with neostigmine increased the duration of EPP somewhat, but did not potentiate the presynaptic effects. No significant changes were observed when neostigmine was given after armin. The possible mechanism of the presynaptic effects of the cholinesterase inhibitors is discussed.", "contents": "Effect of cholinesterase inhibitors on synaptic potentials of the frog neuromuscular junction. The action of neostigmine and armin on the parameters of synaptic potentials of the m. cutaneus pectoris of the frog was investigated. Both drugs caused an approximately equal increase in amplitude of miniature end-plate potentials (MEPP) and end-plate potentials (EPP), and lengthened the rise time and the half-decay time of EPP, but the effect of armin developed faster. The presynaptic action of both inhibitors was demonstrated (an increase in the frequency of MEPP and in the quantum composition of EPP), but it developed much more slowly than the postsynaptic effects; it was more marked in the case of neostigmine. Administration of armin after preliminary contact with neostigmine increased the duration of EPP somewhat, but did not potentiate the presynaptic effects. No significant changes were observed when neostigmine was given after armin. The possible mechanism of the presynaptic effects of the cholinesterase inhibitors is discussed."}
{"id": "PMID:1088635", "title": "The effect of ubiquinone-7 and its metabolites on the immune response. II. Adjuvant activity on the circulating antibody production and the effect on the liver lysosomal membrane.", "content": "The effects of the fat-soluble vitamins (retinol, alpha-tocopherol, phylloquinone and ubiquinone) and their related compounds were investigated on the immune response in vivo. E acid-I, dihydro E acid-I, ES-6, K acid-I, Q acid-II and QS-3 act as adjuvants on the production of circulating antibody to bacterial alpha-amylase in mice when they are immunized with in the water phase of water-in-oil emulsion. These compounds, however, can not act as adjuvants in enhancing helper activity of carrier-primed T-cells using an adoptive transfer system in mice. It is also shown that these compounds have no adjuvant activity on the development of delayed type hypersensitivity to ABA-N-acetyltyrosine in guinea pigs. No definite correlation between the adjuvant activity of these compounds and their labilizing activity on rat-liver lysosomal membrane was found.", "contents": "The effect of ubiquinone-7 and its metabolites on the immune response. II. Adjuvant activity on the circulating antibody production and the effect on the liver lysosomal membrane. The effects of the fat-soluble vitamins (retinol, alpha-tocopherol, phylloquinone and ubiquinone) and their related compounds were investigated on the immune response in vivo. E acid-I, dihydro E acid-I, ES-6, K acid-I, Q acid-II and QS-3 act as adjuvants on the production of circulating antibody to bacterial alpha-amylase in mice when they are immunized with in the water phase of water-in-oil emulsion. These compounds, however, can not act as adjuvants in enhancing helper activity of carrier-primed T-cells using an adoptive transfer system in mice. It is also shown that these compounds have no adjuvant activity on the development of delayed type hypersensitivity to ABA-N-acetyltyrosine in guinea pigs. No definite correlation between the adjuvant activity of these compounds and their labilizing activity on rat-liver lysosomal membrane was found."}
{"id": "PMID:1088637", "title": "ad and ay subtypes of hepatitis B antigen in a case of hypogammaglobulinaemia.", "content": "We describe the finding of d and y specificities of hepatitis B surface antigen (HBsAg) in a case of hypogammaglobulinaemia of the 'common variable' type treated with fresh frozen plasma infusions. Absorption studies show that the two specificities are on separate particles, suggesting dual infection. It raises important questions regarding the relationship between HBsAg persistence and the immune status of the carrier.", "contents": "ad and ay subtypes of hepatitis B antigen in a case of hypogammaglobulinaemia. We describe the finding of d and y specificities of hepatitis B surface antigen (HBsAg) in a case of hypogammaglobulinaemia of the 'common variable' type treated with fresh frozen plasma infusions. Absorption studies show that the two specificities are on separate particles, suggesting dual infection. It raises important questions regarding the relationship between HBsAg persistence and the immune status of the carrier."}
{"id": "PMID:1088641", "title": "Retroperitoneal injuries of the duodenum caused by blunt abdominal trauma.", "content": "Five patients with blunt retroperitoneal injury of the duodenum are presented. In three of them the trauma was caused by a traffic accident, the most common mechanism of such injuries. One patient died. The high index of suspicion is still the best indication for laparotomy in retroperitoneal injuries of the duodenum, because no typical finding or reliably diagnostic test could be demonstrated even in the present study. The proper treatment in less severe injuries of the retroperitoneal duodenum is evacuation of the hematoma or simple suture of the rupture with drainage and naso-gastric suction. Internal drainage as an afferent jejunostomy is sometimes necessary in the treatment of more severe injuries. Pancreatoduodenectomy is to be reserved for only the very severe duodenal injuries, where the head of the pancreas is badly crushed.", "contents": "Retroperitoneal injuries of the duodenum caused by blunt abdominal trauma. Five patients with blunt retroperitoneal injury of the duodenum are presented. In three of them the trauma was caused by a traffic accident, the most common mechanism of such injuries. One patient died. The high index of suspicion is still the best indication for laparotomy in retroperitoneal injuries of the duodenum, because no typical finding or reliably diagnostic test could be demonstrated even in the present study. The proper treatment in less severe injuries of the retroperitoneal duodenum is evacuation of the hematoma or simple suture of the rupture with drainage and naso-gastric suction. Internal drainage as an afferent jejunostomy is sometimes necessary in the treatment of more severe injuries. Pancreatoduodenectomy is to be reserved for only the very severe duodenal injuries, where the head of the pancreas is badly crushed."}
{"id": "PMID:1088709", "title": "Fractionation of human blood lymphocytes on Helix pomatia A haemagglutinin coupled to sepharose beads.", "content": "Treatment of human blood lymphocytes with neuraminidase has previously been shown to uncover receptors for the A haemagglutinin of the snail Helix pomatia (HP). Neuraminidase-treated lymphocytes were now fractionated on columns charged with large Sepharose particles to which HP had been coupled covalently. HP-receptor negative (HP-) lymphocytes passed the columns while HP-receptor positive (HP+) lymphocytes were retained. The latter cells were eluted by addition of the competitive hapten N-acetyl-D-galactosamine (D-GalNAc). The total yield of cells recovered after fractional was 60-80% Surface marker studies indicated that there was no selective loss of any of the major lymphocyte subpopulations. The fraction that passed the columns (fraction I) consisted of approximately 10% of all lymphocytes. It contained approximately 1% HP+cells and approximately 3% of all lymphocytes forming rosettes which sheep erythrocytes (E+ cells) present before fractionation. 50-55% of the lymphocytes in this fraction had surface-bound immunoglobulin (SIg+ cells) and complement receptors (EAC+ cells). Of the SIg+ cells, approximately 60% were true B cells while the remaining 40% had IgG adsorbed to their surface. The majority of the B cells were recovered in this fraction. The lymphocytes of this fraction responded poorly to T-cell mitogen but had an enhanced K-cell activity to chicken erythrocytes. Elution of the cells retained on the column with 0.1 mg/ml D-GalNAc gave a fraction II, consisting of approximately 15% of all lymphocytes. This fraction had a mixed composition. The majority of the cells (approximately 45%) were recovered by subsequent elution with 1.0 mg/ml D-GalNAc. This fraction III was strongly enriched with HP+ and E+ cells (T cells). About 10% of the HP+ cells in this fraction were SIg+. However, on the majority of these cells this surface-bound immunoglobulin was probably externally absorbed IgG. These HP+-SIg\" cells were also EAC+ and had Fc receptors, as shown by rosette formation with IgG-coated bovine erythrocytes. The lymphocytes of fraction III responded most strongly to T-cell mitogen while their K-cell activity was weak.", "contents": "Fractionation of human blood lymphocytes on Helix pomatia A haemagglutinin coupled to sepharose beads. Treatment of human blood lymphocytes with neuraminidase has previously been shown to uncover receptors for the A haemagglutinin of the snail Helix pomatia (HP). Neuraminidase-treated lymphocytes were now fractionated on columns charged with large Sepharose particles to which HP had been coupled covalently. HP-receptor negative (HP-) lymphocytes passed the columns while HP-receptor positive (HP+) lymphocytes were retained. The latter cells were eluted by addition of the competitive hapten N-acetyl-D-galactosamine (D-GalNAc). The total yield of cells recovered after fractional was 60-80% Surface marker studies indicated that there was no selective loss of any of the major lymphocyte subpopulations. The fraction that passed the columns (fraction I) consisted of approximately 10% of all lymphocytes. It contained approximately 1% HP+cells and approximately 3% of all lymphocytes forming rosettes which sheep erythrocytes (E+ cells) present before fractionation. 50-55% of the lymphocytes in this fraction had surface-bound immunoglobulin (SIg+ cells) and complement receptors (EAC+ cells). Of the SIg+ cells, approximately 60% were true B cells while the remaining 40% had IgG adsorbed to their surface. The majority of the B cells were recovered in this fraction. The lymphocytes of this fraction responded poorly to T-cell mitogen but had an enhanced K-cell activity to chicken erythrocytes. Elution of the cells retained on the column with 0.1 mg/ml D-GalNAc gave a fraction II, consisting of approximately 15% of all lymphocytes. This fraction had a mixed composition. The majority of the cells (approximately 45%) were recovered by subsequent elution with 1.0 mg/ml D-GalNAc. This fraction III was strongly enriched with HP+ and E+ cells (T cells). About 10% of the HP+ cells in this fraction were SIg+. However, on the majority of these cells this surface-bound immunoglobulin was probably externally absorbed IgG. These HP+-SIg\" cells were also EAC+ and had Fc receptors, as shown by rosette formation with IgG-coated bovine erythrocytes. The lymphocytes of fraction III responded most strongly to T-cell mitogen while their K-cell activity was weak."}
{"id": "PMID:1088710", "title": "SRBC rosette formation as a human T lymphocyte marker.", "content": "This paper describes a technique utilizing rosette formation with sheep red blood cells as a human T lymphocyte marker, and separation of T cells by rosette sedimentation.", "contents": "SRBC rosette formation as a human T lymphocyte marker. This paper describes a technique utilizing rosette formation with sheep red blood cells as a human T lymphocyte marker, and separation of T cells by rosette sedimentation."}
{"id": "PMID:1088711", "title": "Lymphocyte binding of aggregated immunoglobulin.", "content": "This paper describes lymphocyte binding of aggregated immunoglobulin, which appears to bind to B lymphocytes and the third lymphocyte-like cell population. The applications of aggregated Ig binding are also discussed, and the relation to Fc receptors and Ia-like antigens.", "contents": "Lymphocyte binding of aggregated immunoglobulin. This paper describes lymphocyte binding of aggregated immunoglobulin, which appears to bind to B lymphocytes and the third lymphocyte-like cell population. The applications of aggregated Ig binding are also discussed, and the relation to Fc receptors and Ia-like antigens."}
{"id": "PMID:1088716", "title": "The role of macrophages in the generation of T helper cells. III. Influence of macrophage-derived factors in helper cell induction.", "content": "Helper cell induction to soluble or particulate antigens in vitro requires the cooperation of T cells and macrophages. A direct contact between macrophages and T cells is not obligatory for this cooperation and factors released from macrophages are as effective in activating T cells as the cells themselves. Two different types of macrophage-derived factors where found. The supernatant obtained from purified macrophages incubated with antigen for several days generates helper cells in absence of macrophages or additional antigen, but only if obtained from macrophages which were identical at the I-A subregion of the H-2 complex as the T cells. This factor was called genetically related macrophages factor (GRF). The other factor(s), which is present in the supernatant obtained from macrophages incubated for several days without antigen, replaces macrophages only if the antigen is particulate. This factor(s), called nonspecific macrophage factor (NMF) is not restricted genetically and is also obtained from allogeneic macrophages. The importance of both these factors in helper cell induction is discussed.", "contents": "The role of macrophages in the generation of T helper cells. III. Influence of macrophage-derived factors in helper cell induction. Helper cell induction to soluble or particulate antigens in vitro requires the cooperation of T cells and macrophages. A direct contact between macrophages and T cells is not obligatory for this cooperation and factors released from macrophages are as effective in activating T cells as the cells themselves. Two different types of macrophage-derived factors where found. The supernatant obtained from purified macrophages incubated with antigen for several days generates helper cells in absence of macrophages or additional antigen, but only if obtained from macrophages which were identical at the I-A subregion of the H-2 complex as the T cells. This factor was called genetically related macrophages factor (GRF). The other factor(s), which is present in the supernatant obtained from macrophages incubated for several days without antigen, replaces macrophages only if the antigen is particulate. This factor(s), called nonspecific macrophage factor (NMF) is not restricted genetically and is also obtained from allogeneic macrophages. The importance of both these factors in helper cell induction is discussed."}
{"id": "PMID:1088717", "title": "Selective intraarterial infusion of vasopressin for control of gastrointestinal bleeding: experience with 35 cases.", "content": "Selective intraarterial infusion of vasopressin was performed in 32 patients for 35 episodes of gastrointestinal bleeding. Active bleeding was from esophageal varices in 11 cases and from an arterial site in 22 (stomach 11, duodenum 1, jejunum 2, colon 7, liver 1), including a jejunal diverticulum and a colonic ulcer in Behcet's disease. Two patients, not actively bleeding, were infused for portal decompression before an elective mesocaval shunt. Active bleeding was controlled in 64% of patients with variceal hemorrhage and in 59% of those with arterial sources. Infusion periods ranged from 15 minutes to 70 hours. There were no significant complications directly attributable to this therapy.", "contents": "Selective intraarterial infusion of vasopressin for control of gastrointestinal bleeding: experience with 35 cases. Selective intraarterial infusion of vasopressin was performed in 32 patients for 35 episodes of gastrointestinal bleeding. Active bleeding was from esophageal varices in 11 cases and from an arterial site in 22 (stomach 11, duodenum 1, jejunum 2, colon 7, liver 1), including a jejunal diverticulum and a colonic ulcer in Behcet's disease. Two patients, not actively bleeding, were infused for portal decompression before an elective mesocaval shunt. Active bleeding was controlled in 64% of patients with variceal hemorrhage and in 59% of those with arterial sources. Infusion periods ranged from 15 minutes to 70 hours. There were no significant complications directly attributable to this therapy."}
{"id": "PMID:1088718", "title": "Intramural duodenal hematoma in children.  The role of the radiologist in its conservative management.", "content": "Excellent results can be obtained with conservative therapy of intramural duodenal hematomas, and thus the radiologist plays a crucial role in their management and followup. Six children in in whom intramural duodenal hematoma was diagnosed, based on clinical, laboratory, and radiographic findings, were managed conservatively. Treatment consisted of restricted oral intake, intravenous infusion of fluids and electrolytes, and nasogastric suction when indicated. The satisfactory response of these patients to nonoperative therapy was demonstrated on serial upper gastrointestinal studies and by regression of clinical signs and symptoms.", "contents": "Intramural duodenal hematoma in children.  The role of the radiologist in its conservative management. Excellent results can be obtained with conservative therapy of intramural duodenal hematomas, and thus the radiologist plays a crucial role in their management and followup. Six children in in whom intramural duodenal hematoma was diagnosed, based on clinical, laboratory, and radiographic findings, were managed conservatively. Treatment consisted of restricted oral intake, intravenous infusion of fluids and electrolytes, and nasogastric suction when indicated. The satisfactory response of these patients to nonoperative therapy was demonstrated on serial upper gastrointestinal studies and by regression of clinical signs and symptoms."}
{"id": "PMID:1088719", "title": "Intramural gastrointestinal hemorrhage. Clinical and radiographic manifestations.", "content": "The clinical and radiographic manifestations of intramural gastrointestinal hemorrhage are presented together with a review of the literature. The observations described are based on the analysis of 20 cases affecting different segments of the gastrointestinal tract. The pathologic process is induced principally by anticoagulant therapy, bleeding diathesis, or abdominal trauma and occurs as a localized, well-defined, intramural mass or as a more diffuse segmental involvement. The clinical features are nonspecific and can vary from mild crampy abdominal pain to severe shock. Other manifestations include rebound tenderness, muscle guarding, and small bowel obstruction. The duodenum and small bowel are the most frequent sites of involvement. The intestinal mucosal folds may be prominent, stretched, and sharply defined or completely affaced, accompanied by a smooth narrowing. There is rapid clinical and radiographic remission with conservative therapy. Intestinal perforations and fibrotic strictures resulting in small bowel obstruction are a rarity, observed only in the posttraumatic variety.", "contents": "Intramural gastrointestinal hemorrhage. Clinical and radiographic manifestations. The clinical and radiographic manifestations of intramural gastrointestinal hemorrhage are presented together with a review of the literature. The observations described are based on the analysis of 20 cases affecting different segments of the gastrointestinal tract. The pathologic process is induced principally by anticoagulant therapy, bleeding diathesis, or abdominal trauma and occurs as a localized, well-defined, intramural mass or as a more diffuse segmental involvement. The clinical features are nonspecific and can vary from mild crampy abdominal pain to severe shock. Other manifestations include rebound tenderness, muscle guarding, and small bowel obstruction. The duodenum and small bowel are the most frequent sites of involvement. The intestinal mucosal folds may be prominent, stretched, and sharply defined or completely affaced, accompanied by a smooth narrowing. There is rapid clinical and radiographic remission with conservative therapy. Intestinal perforations and fibrotic strictures resulting in small bowel obstruction are a rarity, observed only in the posttraumatic variety."}
{"id": "PMID:1088740", "title": "Hypertension and color blindness in young men.", "content": "Medical data from Selective Service registrants born from 1939 to 1941 were studied in Oregon and Colorado. Among 29,119 registrants with medical information (41.3% of all registrants), 1,073 (3.6%) had definite hypertension, and 1,226 (4.2%) had some type of color blindness. In both states, there was a highly significant association between the prevalence of hypertension and the prevalence of color blindness. Thus, definite hypertension was present in 6.0% of color clind individuals but in only 3.6% of those with unimparied color vision, while color blindness occurred in 6.8% with definite hypertension, in 5.8% with borderline hypertension, and in only 4.0% with normal blood pressure. The data did not differentiate among types of color blindness, and the reason for the association is not yet evident.", "contents": "Hypertension and color blindness in young men. Medical data from Selective Service registrants born from 1939 to 1941 were studied in Oregon and Colorado. Among 29,119 registrants with medical information (41.3% of all registrants), 1,073 (3.6%) had definite hypertension, and 1,226 (4.2%) had some type of color blindness. In both states, there was a highly significant association between the prevalence of hypertension and the prevalence of color blindness. Thus, definite hypertension was present in 6.0% of color clind individuals but in only 3.6% of those with unimparied color vision, while color blindness occurred in 6.8% with definite hypertension, in 5.8% with borderline hypertension, and in only 4.0% with normal blood pressure. The data did not differentiate among types of color blindness, and the reason for the association is not yet evident."}
{"id": "PMID:1088803", "title": "Oral complications of cancer chemotherapy.", "content": "No part of the body reflects the complications of cancer chemotherapy as visibly and vividly as the mouth. The hemorrhagic, infectious, nutritional, cytotoxic, and neurologic signs of drug toxicity are registered in the mouth by changes in the color, character, and continuity of the mucosa. Although some of the complications are an inevitable part of the price of treatment, each constitutes a threat that must be kept within manageable limits.", "contents": "Oral complications of cancer chemotherapy. No part of the body reflects the complications of cancer chemotherapy as visibly and vividly as the mouth. The hemorrhagic, infectious, nutritional, cytotoxic, and neurologic signs of drug toxicity are registered in the mouth by changes in the color, character, and continuity of the mucosa. Although some of the complications are an inevitable part of the price of treatment, each constitutes a threat that must be kept within manageable limits."}
{"id": "PMID:1088805", "title": "A typical case study correcting color deficiency.", "content": "Within one month after visiting his optometrist, a 14-year-old color deficient vocational high school student was able to sufficiently improve his color discrimination and his perception of objects which blend with their background, so as to continue with his study in electronics. The three prescribed sessions were adequate and the student was very pleased with his color improvement.", "contents": "A typical case study correcting color deficiency. Within one month after visiting his optometrist, a 14-year-old color deficient vocational high school student was able to sufficiently improve his color discrimination and his perception of objects which blend with their background, so as to continue with his study in electronics. The three prescribed sessions were adequate and the student was very pleased with his color improvement."}
{"id": "PMID:1088804", "title": "Intravitreal antibiotic injection and vitrectomy in acute bacterial endophthalmitis.", "content": "Two days after an unplanned extra-capsular cataract extraction with sector iridectomy, a patient showed typical signs of bacterial endophthalmitis. Vitreous and aqueous were aspirated for culture, and gentamicin and dexamethasone were injected. Twenty-four hours later, after isolation of Staphylococcus epidermidis, a vitrectomy to remove the central vitreous was done. Postoperatively, vision progressively improved; at the last review nine months after vitrectomy, visual acuity was 20/50.", "contents": "Intravitreal antibiotic injection and vitrectomy in acute bacterial endophthalmitis. Two days after an unplanned extra-capsular cataract extraction with sector iridectomy, a patient showed typical signs of bacterial endophthalmitis. Vitreous and aqueous were aspirated for culture, and gentamicin and dexamethasone were injected. Twenty-four hours later, after isolation of Staphylococcus epidermidis, a vitrectomy to remove the central vitreous was done. Postoperatively, vision progressively improved; at the last review nine months after vitrectomy, visual acuity was 20/50."}
{"id": "PMID:1088798", "title": "[Isolation and characterization of photochemical properties of the photosynthetic reaction centers from Rhodopseudomonas shperoides, strain 1760-1].", "content": "Photosynthetic reaction centres were isolated from the cells of Rhodopseudomonas spheroides, strain 1760-1, using sodium dodecyl sulphate. The preparations purified by precipitation with ammonium sulphate showed absorbance ratios of A280 : A800=2.1. and A765 : A800 : A870=1 : 2 : 1; about 75% of the bacteriochlorophyll absorbing at 870 nm (P870) were photochemically active. Both absolute and difference \"light minus dark\" absorption spectra were obtained for the reaction centre suspensions and vacuum-dried films at room and low temperatures. Shift to the longer wavelength of the 870 nm absorption band resulting from temperature lowering suggests the existence of temperature-determined conformations of the bacteriochlorophyll-protein complex of the reaction centres. Characteristic time of an electron transfer from the photoexcited P870 to the primary intermediate of photochemical process as evaluated from the data of pulsed laser fluorometry of the reaction centres was found to be (21--15)+/-8 picoseconds. The oxidized P870 dark reduction kinetics dependence on the actinic light intensity gives evidence for the functioning of heterogeneous pool of the secondary electron acceptors in the reaction centre preparations. Filling in of this pool with electrons is decreased under temperature lowering or vacuum drying and its electron capacity is limited under isooctane treatment resulting in ubiquinon extraction. The ability of the reaction centre preparations to catalyze the photochemical oxidation of iminoxyl aromatic radical was demonstrated.", "contents": "[Isolation and characterization of photochemical properties of the photosynthetic reaction centers from Rhodopseudomonas shperoides, strain 1760-1]. Photosynthetic reaction centres were isolated from the cells of Rhodopseudomonas spheroides, strain 1760-1, using sodium dodecyl sulphate. The preparations purified by precipitation with ammonium sulphate showed absorbance ratios of A280 : A800=2.1. and A765 : A800 : A870=1 : 2 : 1; about 75% of the bacteriochlorophyll absorbing at 870 nm (P870) were photochemically active. Both absolute and difference \"light minus dark\" absorption spectra were obtained for the reaction centre suspensions and vacuum-dried films at room and low temperatures. Shift to the longer wavelength of the 870 nm absorption band resulting from temperature lowering suggests the existence of temperature-determined conformations of the bacteriochlorophyll-protein complex of the reaction centres. Characteristic time of an electron transfer from the photoexcited P870 to the primary intermediate of photochemical process as evaluated from the data of pulsed laser fluorometry of the reaction centres was found to be (21--15)+/-8 picoseconds. The oxidized P870 dark reduction kinetics dependence on the actinic light intensity gives evidence for the functioning of heterogeneous pool of the secondary electron acceptors in the reaction centre preparations. Filling in of this pool with electrons is decreased under temperature lowering or vacuum drying and its electron capacity is limited under isooctane treatment resulting in ubiquinon extraction. The ability of the reaction centre preparations to catalyze the photochemical oxidation of iminoxyl aromatic radical was demonstrated."}
{"id": "PMID:1088800", "title": "[Chromatographic fractionation of scraping components of frog olfactory tissues. Preparation of fractions capable of sensitizing artificial lipid membrane to odorants].", "content": "The fractionation of frog olfactory preparation by ion-exchange chromatography and gel filtration permitted to obtain fractions capable of making artificial lipid membranes sensitive to odorants, such as camphora, musc ambrette and linalool. The sensitizing agent present in active fractions is a high-molecular-weight (m.w. 100,000) protein containing substance. It is suggested that this agent is a component of a special transport system which may carry the odorous molecules to olfactory receptor cells or to remove them from olfactory tissues.", "contents": "[Chromatographic fractionation of scraping components of frog olfactory tissues. Preparation of fractions capable of sensitizing artificial lipid membrane to odorants]. The fractionation of frog olfactory preparation by ion-exchange chromatography and gel filtration permitted to obtain fractions capable of making artificial lipid membranes sensitive to odorants, such as camphora, musc ambrette and linalool. The sensitizing agent present in active fractions is a high-molecular-weight (m.w. 100,000) protein containing substance. It is suggested that this agent is a component of a special transport system which may carry the odorous molecules to olfactory receptor cells or to remove them from olfactory tissues."}
{"id": "PMID:1088824", "title": "Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids.", "content": "1. Malic enzyme has been examined by starch-gel electrophoresis in human cultured fibroblasts and in 60 brain samples. The polymorphism of MEM has been confirmed and the electrophoretic pattern suggests that this enzyme, like MES, is a tetramer. 2. The appearance of the soluble and mitochondrial malic enzymes in mouse and Chinese hamster is described, and the identity of the enzymes in man, mouse and hamster has been established from a study of interspecific somatic cell hybrids. In man and in hamster MES migrates anodal to MEM, whereas in the mouse MES is relatively less anodal than MEM. 3. Segregation of human enzymes in human--mouse and human--hamster somatic cell hybrids confirms the synteny of MES and PGM3. Data from human--mouse hybrids suggest that MEM is probably not syntenic with MES is probably not syntenic with MES in man, nor is it syntenic with GPI.", "contents": "Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids. 1. Malic enzyme has been examined by starch-gel electrophoresis in human cultured fibroblasts and in 60 brain samples. The polymorphism of MEM has been confirmed and the electrophoretic pattern suggests that this enzyme, like MES, is a tetramer. 2. The appearance of the soluble and mitochondrial malic enzymes in mouse and Chinese hamster is described, and the identity of the enzymes in man, mouse and hamster has been established from a study of interspecific somatic cell hybrids. In man and in hamster MES migrates anodal to MEM, whereas in the mouse MES is relatively less anodal than MEM. 3. Segregation of human enzymes in human--mouse and human--hamster somatic cell hybrids confirms the synteny of MES and PGM3. Data from human--mouse hybrids suggest that MEM is probably not syntenic with MES is probably not syntenic with MES in man, nor is it syntenic with GPI."}
{"id": "PMID:1088825", "title": "Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function.", "content": "The nucleotide sequence of the region preceding the transcription initiation site of the tryptophan operon of Escherichia coli was determined. Essentially all of the trp operator precedes the transcribed portion of the operon. The deduced sequence contains the recognition site of endonuclease Hpa I. This site is protected from Hpa I cleavage by RNA polymerase and by trp repressor. Regions of 2-fold symmetry are present in the DNA sequence.", "contents": "Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function. The nucleotide sequence of the region preceding the transcription initiation site of the tryptophan operon of Escherichia coli was determined. Essentially all of the trp operator precedes the transcribed portion of the operon. The deduced sequence contains the recognition site of endonuclease Hpa I. This site is protected from Hpa I cleavage by RNA polymerase and by trp repressor. Regions of 2-fold symmetry are present in the DNA sequence."}
{"id": "PMID:1088828", "title": "Varicella in children with cancer: Seventy-seven cases.", "content": "The purpose of this study was to characterize varicella in childhood cancer patients. Seventeen of the 77 patients reviewed were in remission and off all therapy for 3 to 22 months. No one in this group died from varicella or had evidence of visceral dissemination. Among the remaining 60 patients, all of whom were receiving anticancer theapy when they developed varicella, 19 (32%) had visceral dissemination and 4 died, for a mortality rate of 7%. Each of the deaths was associated with primary varicella pneumonitis, with or without acute encephalitis. Visceral dissemination was not related to type or status of malignancy or to duration of specific anticancer therapy. Varicella was more likely to disseminate in children with absolute lymphopenia, less than 500 cells per cubic millimeter, than in patients with higher lymphocyte counts. Cessation of anticancer theapy prior to the onset of lesions appeared to lessen the risk of dissemination. These results show that varicella is more severe in cancer patients on therapy than the general population or in patients who have completed therapy, but is not highly fatal.", "contents": "Varicella in children with cancer: Seventy-seven cases. The purpose of this study was to characterize varicella in childhood cancer patients. Seventeen of the 77 patients reviewed were in remission and off all therapy for 3 to 22 months. No one in this group died from varicella or had evidence of visceral dissemination. Among the remaining 60 patients, all of whom were receiving anticancer theapy when they developed varicella, 19 (32%) had visceral dissemination and 4 died, for a mortality rate of 7%. Each of the deaths was associated with primary varicella pneumonitis, with or without acute encephalitis. Visceral dissemination was not related to type or status of malignancy or to duration of specific anticancer therapy. Varicella was more likely to disseminate in children with absolute lymphopenia, less than 500 cells per cubic millimeter, than in patients with higher lymphocyte counts. Cessation of anticancer theapy prior to the onset of lesions appeared to lessen the risk of dissemination. These results show that varicella is more severe in cancer patients on therapy than the general population or in patients who have completed therapy, but is not highly fatal."}
{"id": "PMID:1088832", "title": "Alcohol use in the Army: patterns and associated behaviors.", "content": "The authors assessed by questionnaire the alcohol use of 1,873 U.S. soldiers in the United States and Viet Nam. According to operational definitions based on total ethyl alcohol consumption and several behaviors associated with drinking, 7 percent were classed as alcoholics, 5 percent as borderline alcoholics, and 24 percent as potential alcoholics. Contrary to popular stereotypes, there was a disproportionate number of younger and lower ranking soldiers in these three groups; there was also a positive relationship between drinking and use of illicit drugs across groups. The authors state that future studies of alcohol use should include determinations of total ethyl alcohol intake to permit generalizability of their results.", "contents": "Alcohol use in the Army: patterns and associated behaviors. The authors assessed by questionnaire the alcohol use of 1,873 U.S. soldiers in the United States and Viet Nam. According to operational definitions based on total ethyl alcohol consumption and several behaviors associated with drinking, 7 percent were classed as alcoholics, 5 percent as borderline alcoholics, and 24 percent as potential alcoholics. Contrary to popular stereotypes, there was a disproportionate number of younger and lower ranking soldiers in these three groups; there was also a positive relationship between drinking and use of illicit drugs across groups. The authors state that future studies of alcohol use should include determinations of total ethyl alcohol intake to permit generalizability of their results."}
{"id": "PMID:1088833", "title": "Sensorimotor functions and cognitive disturbance in psychiatric patients.", "content": "The authors studied 109 consecutive admissions to an acute psychiatric unit in a general hospital to determine the relationship of specific sensorimotor impairments to cognitive disturbance. The results indicated a strong but not exclusive correlation between neurological impairment and thought disorder as well as between neurological impairment and schizophrenia. The theoretical and etiological implications are discussed, as well as the relationship of these findings to other variables.", "contents": "Sensorimotor functions and cognitive disturbance in psychiatric patients. The authors studied 109 consecutive admissions to an acute psychiatric unit in a general hospital to determine the relationship of specific sensorimotor impairments to cognitive disturbance. The results indicated a strong but not exclusive correlation between neurological impairment and thought disorder as well as between neurological impairment and schizophrenia. The theoretical and etiological implications are discussed, as well as the relationship of these findings to other variables."}
{"id": "PMID:1088834", "title": "Utilization review of treatment for suicide attempters.", "content": "The authors describe an effort to develop criteria for utilization review of treatment for suicide attempters. Explicit criteria proposed by a panel of experts as essential determinants for hospitalization of these patients were compared with actual clinical practice. It was found that according to the experts' criteria (which were operationalized into rating assessments), over half of the outpatient sample should have been hospitalized. After multiple regression analysis was carried out on the criteria, however, four predictors showed that only 20 percent of the outpatients should have been hospitalized. The authors discuss the issues these findings raise about the criteria of the experts, their utility for research, their validity, and their implications for utilization review.", "contents": "Utilization review of treatment for suicide attempters. The authors describe an effort to develop criteria for utilization review of treatment for suicide attempters. Explicit criteria proposed by a panel of experts as essential determinants for hospitalization of these patients were compared with actual clinical practice. It was found that according to the experts' criteria (which were operationalized into rating assessments), over half of the outpatient sample should have been hospitalized. After multiple regression analysis was carried out on the criteria, however, four predictors showed that only 20 percent of the outpatients should have been hospitalized. The authors discuss the issues these findings raise about the criteria of the experts, their utility for research, their validity, and their implications for utilization review."}
{"id": "PMID:1088835", "title": "Obstacles in the treatment of dying patients.", "content": "Theory and practice in the management of the dying patient have moved forward in the past two decades. However, the author believes the benefits of this progress have not reached a large segment of the population of dying patients--those individuals who have a higher level of psychopathology or are from a lower socioeconomic group. Obstacles in the treatment of such patients are described and illustrated by case histories; the author makes specific recommendations related to the care of these more difficult cases.", "contents": "Obstacles in the treatment of dying patients. Theory and practice in the management of the dying patient have moved forward in the past two decades. However, the author believes the benefits of this progress have not reached a large segment of the population of dying patients--those individuals who have a higher level of psychopathology or are from a lower socioeconomic group. Obstacles in the treatment of such patients are described and illustrated by case histories; the author makes specific recommendations related to the care of these more difficult cases."}
{"id": "PMID:1088836", "title": "Psychiatric controversy: man's ethical dimension.", "content": "Although determinism may permeate human existence, man's moral values raise him above the level of an animal and enable him to direct his own life. The author suggests that psychiatrists should influence the patient to exert his will, make conscious choices, and, above all, assume a sense of responsibility for his own actions. In order to free the patient from whatever conditions hinder his will and to help him make choices, psychiatric treatment must consider man's ethical dimension.", "contents": "Psychiatric controversy: man's ethical dimension. Although determinism may permeate human existence, man's moral values raise him above the level of an animal and enable him to direct his own life. The author suggests that psychiatrists should influence the patient to exert his will, make conscious choices, and, above all, assume a sense of responsibility for his own actions. In order to free the patient from whatever conditions hinder his will and to help him make choices, psychiatric treatment must consider man's ethical dimension."}
{"id": "PMID:1088837", "title": "The clinical research ward as a therapeutic community: incompatibilities.", "content": "The authors discuss the numerous incompatibilities between the clinical research ward, with its emphasis on the collection of baseline and treatment data, and the therapeutic milieu environment, with its emphasis on an open door, team orientation, and nonauthoritarianism. They posit that the two orientations may be mutually destructive and that the therapeutic milieu may not be the best treatment setting for patients with schizophrenic or certain affective disorders. Two case histories illustrating these ideas are included.", "contents": "The clinical research ward as a therapeutic community: incompatibilities. The authors discuss the numerous incompatibilities between the clinical research ward, with its emphasis on the collection of baseline and treatment data, and the therapeutic milieu environment, with its emphasis on an open door, team orientation, and nonauthoritarianism. They posit that the two orientations may be mutually destructive and that the therapeutic milieu may not be the best treatment setting for patients with schizophrenic or certain affective disorders. Two case histories illustrating these ideas are included."}
{"id": "PMID:1088838", "title": "Limitations and values of evaluation techniques in psychiatric education.", "content": "The author presents a summary of the major evaluation techniques now available and discusses the values and limitations of each. He concludes that only by the use of many evaluation instruments during the resident's period of training can some estimate of clinical competence be approached. Even with this multiplicity of testing instruments, the validity of predictions of a psychiatrist's performance can be determined only in terms of probabilities.", "contents": "Limitations and values of evaluation techniques in psychiatric education. The author presents a summary of the major evaluation techniques now available and discusses the values and limitations of each. He concludes that only by the use of many evaluation instruments during the resident's period of training can some estimate of clinical competence be approached. Even with this multiplicity of testing instruments, the validity of predictions of a psychiatrist's performance can be determined only in terms of probabilities."}
{"id": "PMID:1088839", "title": "The psychiatrist's involvement with aged patients.", "content": "The author studied via questionnaire psychiatrists' involvement with older patients in relation to their own ages and the nature of their practices. Older psychiatrists see a higher proportion of older patients, advocate more comprehensive treatment, and find older patients more interesting and gratifying. Psychiatrists in general psychiatry and those who have a primarily inpatient practice see the largest proportion of older patients.", "contents": "The psychiatrist's involvement with aged patients. The author studied via questionnaire psychiatrists' involvement with older patients in relation to their own ages and the nature of their practices. Older psychiatrists see a higher proportion of older patients, advocate more comprehensive treatment, and find older patients more interesting and gratifying. Psychiatrists in general psychiatry and those who have a primarily inpatient practice see the largest proportion of older patients."}
{"id": "PMID:1088840", "title": "Saliva lithium levels: clinical applications.", "content": "The authors note that despite a few reports in the literature indicating that saliva lithium levels could adequately replace serum determinations in monitoring patients being treated with lithium salts, this procedure has not received much clinical application. A study of 20 patients investigated correlations between serum and mixed saliva and parotid fluid lithium levels and documented the reliability of this procedure. The authors suggest that an individual patient's ratio of serum to saliva concentrations should be calculated and used as a constant in the determinations. Patients can collect saliva samples and send them to the laboratory prior to their visits, decreasing the facility's sample collection costs and the patients' inconvenience and discomfort.", "contents": "Saliva lithium levels: clinical applications. The authors note that despite a few reports in the literature indicating that saliva lithium levels could adequately replace serum determinations in monitoring patients being treated with lithium salts, this procedure has not received much clinical application. A study of 20 patients investigated correlations between serum and mixed saliva and parotid fluid lithium levels and documented the reliability of this procedure. The authors suggest that an individual patient's ratio of serum to saliva concentrations should be calculated and used as a constant in the determinations. Patients can collect saliva samples and send them to the laboratory prior to their visits, decreasing the facility's sample collection costs and the patients' inconvenience and discomfort."}
{"id": "PMID:1088841", "title": "Reasoned discourse or a holy war: postscript to a report on ECT.", "content": "The author points out that both favorable and unfavorable opinions regarding the value of electroconvulsive therapy have become entrenched in the absence of adequate data. These opinions are discussed in relation to the training, experience, and personal orientation of the psychiatrist. The inability of some psychiatrists to tolerate uncertainty is held responsible for their denial of the validity of alternative methods of treatment. The author recommends that psychiatrists tolerate a certain amount of ambiguity and uncertainty: postponing closure may lead to the conclusion that treatment styles (including use of psychotherapy and ECT) are not mutually exclusive.", "contents": "Reasoned discourse or a holy war: postscript to a report on ECT. The author points out that both favorable and unfavorable opinions regarding the value of electroconvulsive therapy have become entrenched in the absence of adequate data. These opinions are discussed in relation to the training, experience, and personal orientation of the psychiatrist. The inability of some psychiatrists to tolerate uncertainty is held responsible for their denial of the validity of alternative methods of treatment. The author recommends that psychiatrists tolerate a certain amount of ambiguity and uncertainty: postponing closure may lead to the conclusion that treatment styles (including use of psychotherapy and ECT) are not mutually exclusive."}
{"id": "PMID:1088842", "title": "How to avoid taking the boards but save face.", "content": "The author present several options for other psychiatrists who wish to avoid taking board examinations and still save face. Although the author states that none of these worked for him, he wishes to present his suggestions to others in the field.", "contents": "How to avoid taking the boards but save face. The author present several options for other psychiatrists who wish to avoid taking board examinations and still save face. Although the author states that none of these worked for him, he wishes to present his suggestions to others in the field."}
{"id": "PMID:1088843", "title": "The curandero's apprentice: a therapeutic integration of folk and medical healing.", "content": "The author discusses alternative methods for dealing with folk illness in psychotherapy. Two case reports are presented that describe psychotic Mexican-American patients who were successfully treated with an approach that integrated curanderismo, the Hispanic concept of healing, and traditional therapy. The author discusses ethical aspects of this integrated approach and suggests that cultural considerations can be successfully incorporated into a treatment plan of optimum benefit for the individual patient.", "contents": "The curandero's apprentice: a therapeutic integration of folk and medical healing. The author discusses alternative methods for dealing with folk illness in psychotherapy. Two case reports are presented that describe psychotic Mexican-American patients who were successfully treated with an approach that integrated curanderismo, the Hispanic concept of healing, and traditional therapy. The author discusses ethical aspects of this integrated approach and suggests that cultural considerations can be successfully incorporated into a treatment plan of optimum benefit for the individual patient."}
{"id": "PMID:1088851", "title": "Assessment of left ventricular wall motion.", "content": "A new method for the assessment of left ventricular wall motion, using a polar analysis, is proposed. The percent shortening of several radii, labeled at 30 degrees intervals, were plotted against Theta (degrees) and an excellent numerical and visual display of wall motion was obtained. Two methods for correcting the systolic upward shifting of apex were tested. The simplest one uses the intersection of the major and minor axes to realign both the end-diastolic and the end-systolic silhouettes. In the other method ventricular outlines were realigned using anatomical internal markers (diaphragm, ribs, or vertebrae). The intersection of both long axes, whether inside or outside the ventricular silhouettes was used as a pivot point around which the long axes were rotated and the outlines realigned. The mid-point of the long axis was used as a common center. There was no statistical difference in the two methods. The data from 14 normal subjects composed a normal percent shortening vs. Theta graph, which can be used as a frame of reference as compared with abnormal hearts.", "contents": "Assessment of left ventricular wall motion. A new method for the assessment of left ventricular wall motion, using a polar analysis, is proposed. The percent shortening of several radii, labeled at 30 degrees intervals, were plotted against Theta (degrees) and an excellent numerical and visual display of wall motion was obtained. Two methods for correcting the systolic upward shifting of apex were tested. The simplest one uses the intersection of the major and minor axes to realign both the end-diastolic and the end-systolic silhouettes. In the other method ventricular outlines were realigned using anatomical internal markers (diaphragm, ribs, or vertebrae). The intersection of both long axes, whether inside or outside the ventricular silhouettes was used as a pivot point around which the long axes were rotated and the outlines realigned. The mid-point of the long axis was used as a common center. There was no statistical difference in the two methods. The data from 14 normal subjects composed a normal percent shortening vs. Theta graph, which can be used as a frame of reference as compared with abnormal hearts."}
{"id": "PMID:1088852", "title": "Echocardiography of ventricular septal movement in left bundle branch block with left ventricular diastolic overload.", "content": "Four patients with left bundle branch block (LBBB) and left ventricular volume overload had sustained posterior ventricular septal systolic motion with no early septal posterior systolic motion. There was earlier than normal peak posterior septal systolic motion and earlier than normal anterior systolic septal motion. It is suggested that volume overload of the left ventricle alters ventricular septal motion in left bundle branch block.", "contents": "Echocardiography of ventricular septal movement in left bundle branch block with left ventricular diastolic overload. Four patients with left bundle branch block (LBBB) and left ventricular volume overload had sustained posterior ventricular septal systolic motion with no early septal posterior systolic motion. There was earlier than normal peak posterior septal systolic motion and earlier than normal anterior systolic septal motion. It is suggested that volume overload of the left ventricle alters ventricular septal motion in left bundle branch block."}
{"id": "PMID:1088857", "title": "Primary umbilical adenocarcinoma. A case report and review of literature.", "content": "A case of primary adenocarcinoma of the umbilicus is reported along with a review of the literature and discussion of possible origins of glandular tissue within this area. Multiple connections are present within the umbilicus (vascular, lymphatic, and embryologic) that may give this area access to metastatic lesions. The primary tumors may originate within the usual umbilical tissue (skin and soft tissue). Glands that are not normally present in the region of the umbilicus rarely develop malignant neoplasms. As postulated, glandular tissue may arise either as metaplasia from squamous epithelium or from glandular embryologic rests including omphalomesenteric duct remnants and urachal remnants.", "contents": "Primary umbilical adenocarcinoma. A case report and review of literature. A case of primary adenocarcinoma of the umbilicus is reported along with a review of the literature and discussion of possible origins of glandular tissue within this area. Multiple connections are present within the umbilicus (vascular, lymphatic, and embryologic) that may give this area access to metastatic lesions. The primary tumors may originate within the usual umbilical tissue (skin and soft tissue). Glands that are not normally present in the region of the umbilicus rarely develop malignant neoplasms. As postulated, glandular tissue may arise either as metaplasia from squamous epithelium or from glandular embryologic rests including omphalomesenteric duct remnants and urachal remnants."}
{"id": "PMID:1088853", "title": "[Synergic effects of intra-aortic counterpulsation and dopamine in experimental cardiogenic shock with closed thorax].", "content": "Having established an easily reproducible, stable, technique for producing cardiogenic shock in dogs, and for applying the intraaortic balloon pump (IABP), a complementary study was carried out in six dogs that were treated for cardiogenic shock with the IABP associated with 8 micrograms/kg/min. of continuous intravenous dopamine. The results were compared with those obtained using only the IABP. It was found that dopamine associated with the IABP reduced the left ventricular end-diastolic pressure by 75.3% and increased the dP/dt and Vmax by 90% and 41.6% respectively. The heart rate rose from 134 (+/- 2.45) to 153.5 (+/- 14.13) beats per minute two hours after treatment. There were no changes in the mean aortic pressure and left ventricular systolic pressure, and diuresis was decreased. It is concluded that the IABP alone produces a significant decrease in the preload and increase in the afterload, but when associated with dopamine, no changes are observed in the afterload, but the preload falls considerably. Moreover, myocardial contractility is increased. Therefore dopamine and the IABP used together have synergic beneficial effects.", "contents": "[Synergic effects of intra-aortic counterpulsation and dopamine in experimental cardiogenic shock with closed thorax]. Having established an easily reproducible, stable, technique for producing cardiogenic shock in dogs, and for applying the intraaortic balloon pump (IABP), a complementary study was carried out in six dogs that were treated for cardiogenic shock with the IABP associated with 8 micrograms/kg/min. of continuous intravenous dopamine. The results were compared with those obtained using only the IABP. It was found that dopamine associated with the IABP reduced the left ventricular end-diastolic pressure by 75.3% and increased the dP/dt and Vmax by 90% and 41.6% respectively. The heart rate rose from 134 (+/- 2.45) to 153.5 (+/- 14.13) beats per minute two hours after treatment. There were no changes in the mean aortic pressure and left ventricular systolic pressure, and diuresis was decreased. It is concluded that the IABP alone produces a significant decrease in the preload and increase in the afterload, but when associated with dopamine, no changes are observed in the afterload, but the preload falls considerably. Moreover, myocardial contractility is increased. Therefore dopamine and the IABP used together have synergic beneficial effects."}
{"id": "PMID:1088854", "title": "[Antiarrhythmic effects of verapamil].", "content": "The effect of verapamil were studied on 50 episodes of supraventricular and ventricular tachycardia in 44 patients. An i.v. dose of 0.10 to 0.15 mg/kg. was used. In 5 cases His bundle electrograms were obtained while maximal dp/dt was determined in 7 others. Sinus rhythm was obtained in 21 (81%) of 26 cases of PSVT. In all cases of rapid atrial fibrillation (n-11), an important decrease in the ventricular response was elicited. Of 7 cases of atrial flutter, verapamil induced sinus rhythm in 3 and a significant decrease in the ventricular rate in 3 others. In 2 out of 6 cases ventricular tachycardia reverted to sinus rhythm. The latency time between the injection and the manifestation of the effect ranged from 2 to 4 minutes. A slight and short-lasting depression of dp/dt was observed in all cases studied. Verapamil produced an increase in the A-H interval in 4 of the 5 cases studied with His bundle recordings. Verapamil was found to be a useful drug to suppress PSVT, to decrease the ventricular response in flutter or atrial fibrillation and to convert some ventricular tachycardias to sinus rhythm. Verapamil should be used with caution in previously digitalized patients and is contraindicated when there is S-A node dysfunction as in the tachycardia-bradycardia syndrome.", "contents": "[Antiarrhythmic effects of verapamil]. The effect of verapamil were studied on 50 episodes of supraventricular and ventricular tachycardia in 44 patients. An i.v. dose of 0.10 to 0.15 mg/kg. was used. In 5 cases His bundle electrograms were obtained while maximal dp/dt was determined in 7 others. Sinus rhythm was obtained in 21 (81%) of 26 cases of PSVT. In all cases of rapid atrial fibrillation (n-11), an important decrease in the ventricular response was elicited. Of 7 cases of atrial flutter, verapamil induced sinus rhythm in 3 and a significant decrease in the ventricular rate in 3 others. In 2 out of 6 cases ventricular tachycardia reverted to sinus rhythm. The latency time between the injection and the manifestation of the effect ranged from 2 to 4 minutes. A slight and short-lasting depression of dp/dt was observed in all cases studied. Verapamil produced an increase in the A-H interval in 4 of the 5 cases studied with His bundle recordings. Verapamil was found to be a useful drug to suppress PSVT, to decrease the ventricular response in flutter or atrial fibrillation and to convert some ventricular tachycardias to sinus rhythm. Verapamil should be used with caution in previously digitalized patients and is contraindicated when there is S-A node dysfunction as in the tachycardia-bradycardia syndrome."}
{"id": "PMID:1088858", "title": "Endocardial papillary elastofibromas. Histologic, histochemical, and electron microscopical findings.", "content": "The organization of cellular and extracellular components appeared similar and was distinctive in three endocardial papillary elastofibromas studied. Each tumor papilla contained (1) a dense, central core of collagen and elastic tissue, (2) a peripheral, myxomatous layer with deposits of acid mucopolysaccharides, and (3) an overlying, hyperplastic layer of endothelial cells. Ultrastructural study of one tumor showed that the cells in all three zones had numerous cytoplasmic filaments, 100 Angstroms in diameter, and dilated cisterns of endoplasmic reticulum; endothelial cells also had intercellular junctions and numerous pinocytotic vesicles. The myxomatous stroma varied from amorphous to fibrillar, and the collagenous cores showed focal degeneration. The name \"endocardial papillary elastofibroma\" is suggested to emphasize those features which are most conspicuous and which serve to differentiate this tumor from myxoma.", "contents": "Endocardial papillary elastofibromas. Histologic, histochemical, and electron microscopical findings. The organization of cellular and extracellular components appeared similar and was distinctive in three endocardial papillary elastofibromas studied. Each tumor papilla contained (1) a dense, central core of collagen and elastic tissue, (2) a peripheral, myxomatous layer with deposits of acid mucopolysaccharides, and (3) an overlying, hyperplastic layer of endothelial cells. Ultrastructural study of one tumor showed that the cells in all three zones had numerous cytoplasmic filaments, 100 Angstroms in diameter, and dilated cisterns of endoplasmic reticulum; endothelial cells also had intercellular junctions and numerous pinocytotic vesicles. The myxomatous stroma varied from amorphous to fibrillar, and the collagenous cores showed focal degeneration. The name \"endocardial papillary elastofibroma\" is suggested to emphasize those features which are most conspicuous and which serve to differentiate this tumor from myxoma."}
{"id": "PMID:1088855", "title": "[Clinical and hemodynamic long-term result of substituting the mitral valve using a Beall prosthesis].", "content": "A follow-up study of 31 patients, 1 1/2 years after they underwent a mitral valve replacement with a Beall prosthesis, is reported. Eighty-four per cent of the patients experienced marked improvement of symptoms and are fully active. Late cardiac recatheterization has showed a decrease of pulmonary wedge pressure, both at rest and under exercise. The prosthesis area has been found to be smaller than the original size. Hemolysis related to the prosthesis is of moderate degree and the incidence of systemic embolism was 3.1%.", "contents": "[Clinical and hemodynamic long-term result of substituting the mitral valve using a Beall prosthesis]. A follow-up study of 31 patients, 1 1/2 years after they underwent a mitral valve replacement with a Beall prosthesis, is reported. Eighty-four per cent of the patients experienced marked improvement of symptoms and are fully active. Late cardiac recatheterization has showed a decrease of pulmonary wedge pressure, both at rest and under exercise. The prosthesis area has been found to be smaller than the original size. Hemolysis related to the prosthesis is of moderate degree and the incidence of systemic embolism was 3.1%."}
{"id": "PMID:1088856", "title": "[Is closed mitral commissurotomy still indicated? Patient selection and postoperative problems. Analysis of 221 cases].", "content": "Indications, findings, complications, and immediate results in 221 patients in whom a closed mitral commissurotomy was practiced are presented. Emphasis is placed on the rigidity of the clinical criteria to select these patients with the object of diminishing to a minimum the accidents or problems and unexpected findings. The results are considered satisfactory and comparable to those reported in other great centers. Finally we considered that the results obtained with this criteria for selection were very satisfactory, with reduction of the immediate mortality to 0% in comparison with others reported, and maintaining an index of complications which we consider minimum in its clinical repercussion to the patient and less than in other series.", "contents": "[Is closed mitral commissurotomy still indicated? Patient selection and postoperative problems. Analysis of 221 cases]. Indications, findings, complications, and immediate results in 221 patients in whom a closed mitral commissurotomy was practiced are presented. Emphasis is placed on the rigidity of the clinical criteria to select these patients with the object of diminishing to a minimum the accidents or problems and unexpected findings. The results are considered satisfactory and comparable to those reported in other great centers. Finally we considered that the results obtained with this criteria for selection were very satisfactory, with reduction of the immediate mortality to 0% in comparison with others reported, and maintaining an index of complications which we consider minimum in its clinical repercussion to the patient and less than in other series."}
{"id": "PMID:1088888", "title": "The social control of organizations in the health care area.", "content": "Available data suggest that the influence and autonomy of health care professionals have been declining. Of course, professional impact remains higher in health care than perhaps any other economic sphere, but the locus of much health care decision making has been shifting from independent professionals to employed personnel of large-scale government, hospital, insurance, and research organizations. The question therefore arises as to what shall replace this previous reliance upon individual professional ethics to assure the society that its newly powerful health care organizations are functioning in a desirable manner. In other words, what are to be the preferred mechanisms for socially controlling health care organizations. This paper traces three dominant belief patterns about how the characteristics of health care organizations and their environments produce desired control. It proposes that belief patterns have emphasized (1) the non-profit motives of many health care organizations; (2) the system of interrelationships that surround health care organizations; and (3) the vast differences among health care consumers. Choices from among these models continue to depend less upon knowledge of organizational functioning than upon political dispositions and social fancy.", "contents": "The social control of organizations in the health care area. Available data suggest that the influence and autonomy of health care professionals have been declining. Of course, professional impact remains higher in health care than perhaps any other economic sphere, but the locus of much health care decision making has been shifting from independent professionals to employed personnel of large-scale government, hospital, insurance, and research organizations. The question therefore arises as to what shall replace this previous reliance upon individual professional ethics to assure the society that its newly powerful health care organizations are functioning in a desirable manner. In other words, what are to be the preferred mechanisms for socially controlling health care organizations. This paper traces three dominant belief patterns about how the characteristics of health care organizations and their environments produce desired control. It proposes that belief patterns have emphasized (1) the non-profit motives of many health care organizations; (2) the system of interrelationships that surround health care organizations; and (3) the vast differences among health care consumers. Choices from among these models continue to depend less upon knowledge of organizational functioning than upon political dispositions and social fancy."}
{"id": "PMID:1088893", "title": "New Q waves after bypass grafting: correlations between graft patency, ventriculogram and surgical venting technique.", "content": "New Q waves were observed in 35 (11%) of 321 patients undergoing saphenous vein bypass grafting with an overall mortality rate of 1.1%. Twenty-eight (80%) had postoperative arteriograms and ventriculograms and are reported. Ventricular venting was used intra-operatively in 17 patients and atrial venting in 11. The incidence of new Q wave was 22% in patients with ventricular venting and 5.5% in those with atrial venting (p less than 0.05). Complete or incomplete revascularization did not affect the incidence of new Q waves. New Q waves appeared in a zone of myocardium supplied by a grafted artery in all except two patients with ventricular venting in whom Q waves occurred within the zone of myocardium supplied by diseased ungrafted vessels. In the ventricular venting group, seven (41%) demonstrated an improved or unchanged postoperative ventriculogram and ten (59%) had deteriorated ventriculograms. In 11 patients with atrial venting, nine (82%) showed improved or unchanged postoperative ventriculograms and two (18%) had deteriorated ventriculograms. Ventricular venting patients with improved or unchanged postoperative ventriculograms had 7% graft closure as compared to 5% of those with atrial venting (pNS). Graft closure rate was 44% in ventricular venting and 20% (pNS) of patients with atrial venting who had deteriorated left ventriculograms. These findings indicate poor correlation between new Q waves and graft closure. Improved postoperative ventriculograms corrleated well with graft patency despite new Q waves. The etiology of new post bypass graft Q waves are varied. They include ventricular trauma and conduction delays resulting from surgery or venting, as well as infarction. This may be due to compromised arterial inflow either in nonoperated vessels or in the vessels distal to the anastomosis with patent grafts, or due to occluded grafts.", "contents": "New Q waves after bypass grafting: correlations between graft patency, ventriculogram and surgical venting technique. New Q waves were observed in 35 (11%) of 321 patients undergoing saphenous vein bypass grafting with an overall mortality rate of 1.1%. Twenty-eight (80%) had postoperative arteriograms and ventriculograms and are reported. Ventricular venting was used intra-operatively in 17 patients and atrial venting in 11. The incidence of new Q wave was 22% in patients with ventricular venting and 5.5% in those with atrial venting (p less than 0.05). Complete or incomplete revascularization did not affect the incidence of new Q waves. New Q waves appeared in a zone of myocardium supplied by a grafted artery in all except two patients with ventricular venting in whom Q waves occurred within the zone of myocardium supplied by diseased ungrafted vessels. In the ventricular venting group, seven (41%) demonstrated an improved or unchanged postoperative ventriculogram and ten (59%) had deteriorated ventriculograms. In 11 patients with atrial venting, nine (82%) showed improved or unchanged postoperative ventriculograms and two (18%) had deteriorated ventriculograms. Ventricular venting patients with improved or unchanged postoperative ventriculograms had 7% graft closure as compared to 5% of those with atrial venting (pNS). Graft closure rate was 44% in ventricular venting and 20% (pNS) of patients with atrial venting who had deteriorated left ventriculograms. These findings indicate poor correlation between new Q waves and graft closure. Improved postoperative ventriculograms corrleated well with graft patency despite new Q waves. The etiology of new post bypass graft Q waves are varied. They include ventricular trauma and conduction delays resulting from surgery or venting, as well as infarction. This may be due to compromised arterial inflow either in nonoperated vessels or in the vessels distal to the anastomosis with patent grafts, or due to occluded grafts."}
{"id": "PMID:1088895", "title": "The effects of growth hormone treatment of thyroid-deficient pregnant rats on maternal and fetal carbohydrate metabolism.", "content": "Maternal hypothyroidism in rats has been shown previously to result in alterations of maternal, placental, and fetal metabolism. Maternal treatment with 2 IU GH/day for three days prior to autopsy (on the 22nd day of pregnancy) corrected many of the observed alterations of carbohydrate metabolism in hypothyroidism. The maternal and fetal liver glycogen concentrations and the fetal serum glucose levels of the hypothyroid animals were elevated significantly by the GH treatment. In most cases, the utilization of a [1-14C]glucose tracer dose was returned to normal by GH treatment. These results suggest that the impairment of fetal metabolism occurring in maternal hypothyroidism may be due in part to insufficient maternal GH secretion. However, GH alone in the absence of sufficient thyroid hormones did not totally correct all of the observed fetal abnormalities.", "contents": "The effects of growth hormone treatment of thyroid-deficient pregnant rats on maternal and fetal carbohydrate metabolism. Maternal hypothyroidism in rats has been shown previously to result in alterations of maternal, placental, and fetal metabolism. Maternal treatment with 2 IU GH/day for three days prior to autopsy (on the 22nd day of pregnancy) corrected many of the observed alterations of carbohydrate metabolism in hypothyroidism. The maternal and fetal liver glycogen concentrations and the fetal serum glucose levels of the hypothyroid animals were elevated significantly by the GH treatment. In most cases, the utilization of a [1-14C]glucose tracer dose was returned to normal by GH treatment. These results suggest that the impairment of fetal metabolism occurring in maternal hypothyroidism may be due in part to insufficient maternal GH secretion. However, GH alone in the absence of sufficient thyroid hormones did not totally correct all of the observed fetal abnormalities."}
{"id": "PMID:1088905", "title": "History strikes again: two 20th-century reform ventures.", "content": "There are striking parallels between the rise and decline of the health center movement of the early decades of this century and the community mental health center movement of the sixties. Four ideas that dominated the early health center movement--district location, preventive care, community participation, and bureaucratic organization--were also important in the development of community mental health centers. Both movements initially were expressions of the reform spirit of the times and later were undermined by the shift to conservative domestic politics.", "contents": "History strikes again: two 20th-century reform ventures. There are striking parallels between the rise and decline of the health center movement of the early decades of this century and the community mental health center movement of the sixties. Four ideas that dominated the early health center movement--district location, preventive care, community participation, and bureaucratic organization--were also important in the development of community mental health centers. Both movements initially were expressions of the reform spirit of the times and later were undermined by the shift to conservative domestic politics."}
{"id": "PMID:1088908", "title": "Direct evidence of estrogen modulation of pituitary sensitivity to luteinizing hormone-releasing factor during the menstrual cycle.", "content": "To delineate the role of estradiol in the augmented pituitary gonadotropin responsiveness to synthetic luteinizing hormone releasing factor (LRF) seen during high-estrogen phases of the ovulatory cycles (late follicular and midluteal phases), the anti-estrogenic effect of clomiphene citrate (Clomid) on pituitary response to LRF was evaluated during different phases of the ovulatory cycle. Clomid administration (100 mg/day times 5 days) completely negates the augmented gonadotropin responses to LRF (150 mug) during late follicular and midluteal phases observed during the control studies. Thus, a quantitatively and qualitatively similar pituitary sensitivity to LRF during three distinct phases of the menstrual cycle was induced by Clomid treatment that resembles the LRF responsiveness of themale pituitary. The present study demonstrates the pituitary component of the estrogen-induced changes in the sensitivity to LRF. From this and previous data, we conclude that the increases of estradiol secretion associated with the follicular maturation and corpus luteum formation represent a major component of the feedback signal in the modulation of cyclic gonadotropin release occasioned in a large measure by the augmented pituitary sensitivity to LRF.", "contents": "Direct evidence of estrogen modulation of pituitary sensitivity to luteinizing hormone-releasing factor during the menstrual cycle. To delineate the role of estradiol in the augmented pituitary gonadotropin responsiveness to synthetic luteinizing hormone releasing factor (LRF) seen during high-estrogen phases of the ovulatory cycles (late follicular and midluteal phases), the anti-estrogenic effect of clomiphene citrate (Clomid) on pituitary response to LRF was evaluated during different phases of the ovulatory cycle. Clomid administration (100 mg/day times 5 days) completely negates the augmented gonadotropin responses to LRF (150 mug) during late follicular and midluteal phases observed during the control studies. Thus, a quantitatively and qualitatively similar pituitary sensitivity to LRF during three distinct phases of the menstrual cycle was induced by Clomid treatment that resembles the LRF responsiveness of themale pituitary. The present study demonstrates the pituitary component of the estrogen-induced changes in the sensitivity to LRF. From this and previous data, we conclude that the increases of estradiol secretion associated with the follicular maturation and corpus luteum formation represent a major component of the feedback signal in the modulation of cyclic gonadotropin release occasioned in a large measure by the augmented pituitary sensitivity to LRF."}
{"id": "PMID:1088909", "title": "Partial characterization and purification of a rabbit granulocyte factor that increases permeability of Escherichia coli.", "content": "Recently we reported that rapid killing of Escherichia coli by granulocytes or granulocyte fractions is accompanied by an equally rapid and discrete increase in permeability of the microbial envelope (Beckerdite, Mooney, Weiss, Franson, and Elsbach. 1974. J. Exp. Med. 140: 396-409). Most of this permeability-increasing activity (PI) is found in a crude granule preparation. PI is quantitatively recovered in a 23,000-g supernatant fraction (Sup II) after sulfuric acid extraction of granulocyte homogenates prepared in water. PI is nondialyzable, destroyed by pronase and trypsin, stable at 4degreesC for at least 2 mo, and destroyed by heating at 94degreesC. Anionic substances, such as heparin sulfate and isolated E. coli lipopolysaccharide, bind to and inhibit PI. PI has been purified up to 1,000-fold from homogenate in a yield of 50percent by acid extraction and carboxymethyl-Sephadex chromatography. Such purified fractions have bactericidal activity that equals that of disrupted granulocytes and Sup II, are similarly enriched with respect to granule-associated phospholipase, and protease activities. Whereas E. coli, sensitive to PI, binds or inactivates solubilized PI, a resistant strain of Serratia marcescens does not. Binding of PI to sensitive microorganisms seems to be necessary for expression of its biological activity since both the apparent binding to and the biological effect of PI on E. coli are completely blocked by 10-20 mM Mg2+ or Ca2+. Mg2+ or Ca2+ can reverse the effect on E. coli permeability produced by Sup II or the carboxymethyl-Sephadex fraction but not that produced by granulocyte homogenate. The close association of bactericidal, phospholipase A2, and permeability-increasing activities towards several gram-negative bacterial species suggests that they may be related.", "contents": "Partial characterization and purification of a rabbit granulocyte factor that increases permeability of Escherichia coli. Recently we reported that rapid killing of Escherichia coli by granulocytes or granulocyte fractions is accompanied by an equally rapid and discrete increase in permeability of the microbial envelope (Beckerdite, Mooney, Weiss, Franson, and Elsbach. 1974. J. Exp. Med. 140: 396-409). Most of this permeability-increasing activity (PI) is found in a crude granule preparation. PI is quantitatively recovered in a 23,000-g supernatant fraction (Sup II) after sulfuric acid extraction of granulocyte homogenates prepared in water. PI is nondialyzable, destroyed by pronase and trypsin, stable at 4degreesC for at least 2 mo, and destroyed by heating at 94degreesC. Anionic substances, such as heparin sulfate and isolated E. coli lipopolysaccharide, bind to and inhibit PI. PI has been purified up to 1,000-fold from homogenate in a yield of 50percent by acid extraction and carboxymethyl-Sephadex chromatography. Such purified fractions have bactericidal activity that equals that of disrupted granulocytes and Sup II, are similarly enriched with respect to granule-associated phospholipase, and protease activities. Whereas E. coli, sensitive to PI, binds or inactivates solubilized PI, a resistant strain of Serratia marcescens does not. Binding of PI to sensitive microorganisms seems to be necessary for expression of its biological activity since both the apparent binding to and the biological effect of PI on E. coli are completely blocked by 10-20 mM Mg2+ or Ca2+. Mg2+ or Ca2+ can reverse the effect on E. coli permeability produced by Sup II or the carboxymethyl-Sephadex fraction but not that produced by granulocyte homogenate. The close association of bactericidal, phospholipase A2, and permeability-increasing activities towards several gram-negative bacterial species suggests that they may be related."}
{"id": "PMID:1088910", "title": "Human lymphocyte surface immunoglobulin capping. Normal characteristics and anomalous behavior of chronic lymphocytic leukemic lymphocytes.", "content": "The phenomenon of redistribution of surface membrane immunoglobulin (Ig) components (capping) has been well described in mouse lymphoid cells. The characteristics of this process in human lymphocytes are less clear. This study characterizes the phenomenon of surface membrane Ig redistribution of normal and chronic lymphocytic leukemia (CLL) lymphocytes with the use of fluoroscein-labeled anti-Ig sera. Normal lymphocytes underwent rapid cap formation after incubation with anti-Ig serum in the cold and subsequent rewarming. The morphology was characteristic with aggregation over the pole of the cell opposite the nucleus and over the uropod when present. The process was energy dependent but independent of protein synthesis, and could be inhibited by vincristine, vinblastine, and colchicine but not by cytochalasin B. CLL cells, on the other hand, though showing fluorescent complex aggregation on the surface, rarely demonstrated unidirectional movement of these aggregates to form a cap. Cap formation in these cells could not be stimulated by supplementing the energy source or protein concentration of the medium nor by adding glutamic acid which could partially reverse the vincristine and vinblastine inhibition of normal capping. The failure of agents which inhibit motility to inhibit capping of the normal lymphocytes suggests that active locomotion is not a direct prerequisite for capping. The results also suggest the involvement of microtubules in normal capping and the possibility that abnormal membrane structure or microtubular function could explain the failure of CLL cells to behave normally in this regard. The role of this cellular defect in the immune deficiencies exhibited by many patients with CLL, however, is not established.", "contents": "Human lymphocyte surface immunoglobulin capping. Normal characteristics and anomalous behavior of chronic lymphocytic leukemic lymphocytes. The phenomenon of redistribution of surface membrane immunoglobulin (Ig) components (capping) has been well described in mouse lymphoid cells. The characteristics of this process in human lymphocytes are less clear. This study characterizes the phenomenon of surface membrane Ig redistribution of normal and chronic lymphocytic leukemia (CLL) lymphocytes with the use of fluoroscein-labeled anti-Ig sera. Normal lymphocytes underwent rapid cap formation after incubation with anti-Ig serum in the cold and subsequent rewarming. The morphology was characteristic with aggregation over the pole of the cell opposite the nucleus and over the uropod when present. The process was energy dependent but independent of protein synthesis, and could be inhibited by vincristine, vinblastine, and colchicine but not by cytochalasin B. CLL cells, on the other hand, though showing fluorescent complex aggregation on the surface, rarely demonstrated unidirectional movement of these aggregates to form a cap. Cap formation in these cells could not be stimulated by supplementing the energy source or protein concentration of the medium nor by adding glutamic acid which could partially reverse the vincristine and vinblastine inhibition of normal capping. The failure of agents which inhibit motility to inhibit capping of the normal lymphocytes suggests that active locomotion is not a direct prerequisite for capping. The results also suggest the involvement of microtubules in normal capping and the possibility that abnormal membrane structure or microtubular function could explain the failure of CLL cells to behave normally in this regard. The role of this cellular defect in the immune deficiencies exhibited by many patients with CLL, however, is not established."}
{"id": "PMID:1088911", "title": "Continuing dental education: reasonable answers to unreasonable questions.", "content": "Increasing government involvement in health care, interest in consumerism, and improved instructional technology have raised new challenges for continuing dental education. Arguments from educational research and evaluation theory are used to show the potential dangers involved in compulsory, mass-media continuing education with pre- and posttest measures of success.", "contents": "Continuing dental education: reasonable answers to unreasonable questions. Increasing government involvement in health care, interest in consumerism, and improved instructional technology have raised new challenges for continuing dental education. Arguments from educational research and evaluation theory are used to show the potential dangers involved in compulsory, mass-media continuing education with pre- and posttest measures of success."}
{"id": "PMID:1088912", "title": "Technetium-99m radionuclide bone imaging for evaluating mandibular osseous allografts.", "content": "Sequential interpretation of osseous repair, more sensitive than with conventional radiography, is possible with a noninvasive, nondestructive radio-nuclide method. The method was used in the evaluation of the progress of osteogenic activity in mandibular bone grafts in 24 beagle dogs.", "contents": "Technetium-99m radionuclide bone imaging for evaluating mandibular osseous allografts. Sequential interpretation of osseous repair, more sensitive than with conventional radiography, is possible with a noninvasive, nondestructive radio-nuclide method. The method was used in the evaluation of the progress of osteogenic activity in mandibular bone grafts in 24 beagle dogs."}
{"id": "PMID:1088913", "title": "Sj\u00f6gren's syndrome.", "content": "The diagnosis of Sj\u00f6gren's syndrome, a multi-systemic disease, is often difficult. Sialography is useful; however, biopsy of labial salivary glands is a simple procedure that aids significantly in arriving at a positive diagnosis. Treatment of the disease is usually on the basis of symptoms.", "contents": "Sj\u00f6gren's syndrome. The diagnosis of Sj\u00f6gren's syndrome, a multi-systemic disease, is often difficult. Sialography is useful; however, biopsy of labial salivary glands is a simple procedure that aids significantly in arriving at a positive diagnosis. Treatment of the disease is usually on the basis of symptoms."}
{"id": "PMID:1088916", "title": "Efficacy of an antihypertensive agent. Comparison of methyldopa and hydrochlorothiazide in combination and singly.", "content": "In a double-blind evaluation, the efficacy of a combination of methyldopa with hydrochlorothiazide was compared with that of its components, methyldopa and hydrochlorothiazide. The combination was found to be more efficacious than either of its components. It reduced the arithmetic mean of the pressures of all patients from 170/116 to 133/95 mm Hg in four weeks. Despite the greater effect of the combination, side effects were mild.", "contents": "Efficacy of an antihypertensive agent. Comparison of methyldopa and hydrochlorothiazide in combination and singly. In a double-blind evaluation, the efficacy of a combination of methyldopa with hydrochlorothiazide was compared with that of its components, methyldopa and hydrochlorothiazide. The combination was found to be more efficacious than either of its components. It reduced the arithmetic mean of the pressures of all patients from 170/116 to 133/95 mm Hg in four weeks. Despite the greater effect of the combination, side effects were mild."}
{"id": "PMID:1088926", "title": "Genetic regulation: the Lac control region.", "content": "The nucleotide sequence of the lac promoter-operator region has been determined. The 122 base pairs comprising this region include the recognition sites for RNA polymerase, the positive regulatory protein, CAP, and the negative regulatory protein, the repressor. Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.", "contents": "Genetic regulation: the Lac control region. The nucleotide sequence of the lac promoter-operator region has been determined. The 122 base pairs comprising this region include the recognition sites for RNA polymerase, the positive regulatory protein, CAP, and the negative regulatory protein, the repressor. Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter."}
{"id": "PMID:1088927", "title": "Mitosis in Tilia americana endosperm.", "content": "The endosperm cells of the American basswood Tilia americana are favorable experimental material for investigating the birefringence of living plant spindles and anaphase movement of chromosomes. The behavior of the chromosomes in anaphase and the formation of the phragmoplast are unique. The numerous (3 n equals 123), small chromosomes move in precise, parallel rows until midanaphase when they bow away from the poles. Such a pattern of anaphase chromosome distribution has been described once before, but was ascribed to fusion of the chromosomes. The bowing of chromosome rows in Tilia is explainable quantitatively by the constant poleward velocity of the chromosomes during anaphase. Peripheral chromosomes are moving both relative to the spindle axis and laterally closer to the axis, whereas chromosomes lying on the spindle axis possess no lateral component in their motion, and thus at uniform velocity progress more rapidly than peripheral chromosomes relative to the spindle axis. The chromosomes are moved poleward initially by pole-to-pole elongation of the spindle, then moved farther apart by shortening of the kinetochore fibers. In contrast to other plant cells where the phragmoplast forms in telophase, the phragmoplast in Tilia endosperm is formed before midanaphase and the cell during midanaphase, while the chromosomes are still in poleward transit.", "contents": "Mitosis in Tilia americana endosperm. The endosperm cells of the American basswood Tilia americana are favorable experimental material for investigating the birefringence of living plant spindles and anaphase movement of chromosomes. The behavior of the chromosomes in anaphase and the formation of the phragmoplast are unique. The numerous (3 n equals 123), small chromosomes move in precise, parallel rows until midanaphase when they bow away from the poles. Such a pattern of anaphase chromosome distribution has been described once before, but was ascribed to fusion of the chromosomes. The bowing of chromosome rows in Tilia is explainable quantitatively by the constant poleward velocity of the chromosomes during anaphase. Peripheral chromosomes are moving both relative to the spindle axis and laterally closer to the axis, whereas chromosomes lying on the spindle axis possess no lateral component in their motion, and thus at uniform velocity progress more rapidly than peripheral chromosomes relative to the spindle axis. The chromosomes are moved poleward initially by pole-to-pole elongation of the spindle, then moved farther apart by shortening of the kinetochore fibers. In contrast to other plant cells where the phragmoplast forms in telophase, the phragmoplast in Tilia endosperm is formed before midanaphase and the cell during midanaphase, while the chromosomes are still in poleward transit."}
{"id": "PMID:1088928", "title": "Radionuclides and the patient with coronary artery disease.", "content": "The emergence in the past 20 years of nuclear medicine as a distinct diagnostic discipline has been a major clinical advance. The relatively rapid evolution from the small laboratory limited primarily to the study of thyroid disease to the large unit in which radioactive tracers (radionuclides) are utilized to evaluate structure and function of most organ systems has been accelerated by major advances in instrumentation, new radioactive tracers and application of computer techniques. Application of these radionuclide techniques to the study of coronary artery disease has been quite recent, and has in part been limited by the need for a coordinated effort between the cardiologist and the specialist in nuclear medicine. However, realization of the potential usefulness of these techniques has fostered an increasingly productive liaison between the two specialties. The potential advantages of these radionuclides in evaluating patients with cardiovascular disease is twofold: first, they may permit the noninvasive or atraumatic acquisition of data that might otherwise be obtained only at the time of cardiac catheterization; second, and perhaps more important, they may permit the acquisition of physiologic measurements or observations not attainable by more conventional modes of study. Functionally, these techniques can be divided into those that evaluate cardiac performance and those that evaluate coronary blood flow, regional myocardial perfusion and myocardial viability.", "contents": "Radionuclides and the patient with coronary artery disease. The emergence in the past 20 years of nuclear medicine as a distinct diagnostic discipline has been a major clinical advance. The relatively rapid evolution from the small laboratory limited primarily to the study of thyroid disease to the large unit in which radioactive tracers (radionuclides) are utilized to evaluate structure and function of most organ systems has been accelerated by major advances in instrumentation, new radioactive tracers and application of computer techniques. Application of these radionuclide techniques to the study of coronary artery disease has been quite recent, and has in part been limited by the need for a coordinated effort between the cardiologist and the specialist in nuclear medicine. However, realization of the potential usefulness of these techniques has fostered an increasingly productive liaison between the two specialties. The potential advantages of these radionuclides in evaluating patients with cardiovascular disease is twofold: first, they may permit the noninvasive or atraumatic acquisition of data that might otherwise be obtained only at the time of cardiac catheterization; second, and perhaps more important, they may permit the acquisition of physiologic measurements or observations not attainable by more conventional modes of study. Functionally, these techniques can be divided into those that evaluate cardiac performance and those that evaluate coronary blood flow, regional myocardial perfusion and myocardial viability."}
{"id": "PMID:1088929", "title": "Cardiovascular investigations and fuzzy sets theory.", "content": "Examining the circumstances that often lead modern, mechanistic cardiovascular research into areas devoid of practical significance, we suggest that our approaches, instead of our technology, are inadequate. A method for processing information, built on the concept of \"fuzzy sets,\" is described as a potential way out of this dilemma. This system deserves application to clinical problems, perhaps together with more classic quantitative methods, because of its practical consequences and the conceptual revitalization it may bring.", "contents": "Cardiovascular investigations and fuzzy sets theory. Examining the circumstances that often lead modern, mechanistic cardiovascular research into areas devoid of practical significance, we suggest that our approaches, instead of our technology, are inadequate. A method for processing information, built on the concept of \"fuzzy sets,\" is described as a potential way out of this dilemma. This system deserves application to clinical problems, perhaps together with more classic quantitative methods, because of its practical consequences and the conceptual revitalization it may bring."}
{"id": "PMID:1088936", "title": "Myocardial blood flow response to isometric (handgrip) and treadmill exercise in coronary artery disease.", "content": "Thirty patients undergoing coronary cineangiography for diagnosis or evaluation of coronary artery disease had myocardial blood flow studies pre and post handgrip (isometric) exercise just prior to cineangiography. The handgrip was maintained at one-third maximum effort for three minutes. The patients also had treadmill exercise testing a day or two prior to the study. Treadmill testing was carried out until angina or positive ST-segment changes occurred or the patient attained 90% of predicted maximal heart rate. Of the 30 patients, seven had no coronary artery disease and 23 had significant disease, i.e., greater than 75% occlusion of at least one major coronary artery. Six of the 23 patients (26%) with disease had angina with handgrip and demonstrated a decreased myocardial blood flow, a significantly different response from the patients without agina (P less than 0.001). For the group without angina, the blood flow response was not significantly different from the normal group. Treadmill tests were positive in 19 of the 23 patients with coronary disease (83%). The arterial systolic and diastolic pressure rise was almost identical with the two stresses. The heart rate response, however, was significantly different (P less than 0.001), the rate increase being greater with treadmill exercise. The myocardial blood flow data demonstrate the relative insensitivity of handgrip exercise for the diagnosis of coronary artery disease. In terms of the supply demand ratio (diastolic pressure time/systolic pressure time) concept for subendocardial perfusion, it is possible that the difference between handgrip and treadmill stress may be due to the different heart rate response, more rapid heart rates having a relatively greater effect on diastolic than on systolic time indices, all other factors being equal.", "contents": "Myocardial blood flow response to isometric (handgrip) and treadmill exercise in coronary artery disease. Thirty patients undergoing coronary cineangiography for diagnosis or evaluation of coronary artery disease had myocardial blood flow studies pre and post handgrip (isometric) exercise just prior to cineangiography. The handgrip was maintained at one-third maximum effort for three minutes. The patients also had treadmill exercise testing a day or two prior to the study. Treadmill testing was carried out until angina or positive ST-segment changes occurred or the patient attained 90% of predicted maximal heart rate. Of the 30 patients, seven had no coronary artery disease and 23 had significant disease, i.e., greater than 75% occlusion of at least one major coronary artery. Six of the 23 patients (26%) with disease had angina with handgrip and demonstrated a decreased myocardial blood flow, a significantly different response from the patients without agina (P less than 0.001). For the group without angina, the blood flow response was not significantly different from the normal group. Treadmill tests were positive in 19 of the 23 patients with coronary disease (83%). The arterial systolic and diastolic pressure rise was almost identical with the two stresses. The heart rate response, however, was significantly different (P less than 0.001), the rate increase being greater with treadmill exercise. The myocardial blood flow data demonstrate the relative insensitivity of handgrip exercise for the diagnosis of coronary artery disease. In terms of the supply demand ratio (diastolic pressure time/systolic pressure time) concept for subendocardial perfusion, it is possible that the difference between handgrip and treadmill stress may be due to the different heart rate response, more rapid heart rates having a relatively greater effect on diastolic than on systolic time indices, all other factors being equal."}
{"id": "PMID:1088942", "title": "Changes in monoamine oxidase and monoamines with human development and aging.", "content": "A series of studies of monoamines and their metabolism in a variety of human tissues indicate that there are aging effects that may alter neurotransmitter substances. Monoamine oxidase (MAO) activity has a significant positive correlation with age in plasma and blood platelets of normal subjects and patients suffering from depressive disorders. Monoamine oxidase and age correlate positively in hindbrain and in eight separate ares of human brains from patients who died from a variety of causes. Hindbrain norepinephrine concentration progressively decreases with advancing age (r equals -0.44, P less than 0.01) while no changes were noted for serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA). Hindbrain norepinephrine concentration has a significant negative correlation with MAO (r equals -0.41, P less than 0.025) and hindbrain 5-HIAA has a significant positive correlation with MAO (r equals +0.66, P equals less than 0.05). These studies suggest that aging processes may significantly affect monoamine mechanisms and be a predisposing factor to the development of clinical diseases in man such as depression, parkinsonism and other disorders of central nervous system homeostasis.", "contents": "Changes in monoamine oxidase and monoamines with human development and aging. A series of studies of monoamines and their metabolism in a variety of human tissues indicate that there are aging effects that may alter neurotransmitter substances. Monoamine oxidase (MAO) activity has a significant positive correlation with age in plasma and blood platelets of normal subjects and patients suffering from depressive disorders. Monoamine oxidase and age correlate positively in hindbrain and in eight separate ares of human brains from patients who died from a variety of causes. Hindbrain norepinephrine concentration progressively decreases with advancing age (r equals -0.44, P less than 0.01) while no changes were noted for serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA). Hindbrain norepinephrine concentration has a significant negative correlation with MAO (r equals -0.41, P less than 0.025) and hindbrain 5-HIAA has a significant positive correlation with MAO (r equals +0.66, P equals less than 0.05). These studies suggest that aging processes may significantly affect monoamine mechanisms and be a predisposing factor to the development of clinical diseases in man such as depression, parkinsonism and other disorders of central nervous system homeostasis."}
{"id": "PMID:1088943", "title": "Towards a theory of development.", "content": "A theory of development would effectively enable one to compute the adult organism from the genetic information in the egg. The problem may be approached by viewing the egg as containing a program for development, and considering the logical nature of the program by treating cells as automata and ignoring the details of molecular mechanisms. It is suggested that development is essentially a simple process, the cells having a limited repertoire of overt activities and interacting with each other by means of simple signals, and that general principles may be discerned. The complexity lies in the specification of the internal state which may be described in terms of a gene-switching network. Pattern formation is a central feature in development; it is the process whereby states are assigned to the cells according to their position. such that the appropriate type of cytodifferentiation is selected from the repertoire. The morphogenesis of the chick limb is briefly discussed. Genetic networks that account for such features as memory, competence and interpretation of positional information are given. The question of how these component parts are organized into a complete control system for development is posed as a problem for future study.", "contents": "Towards a theory of development. A theory of development would effectively enable one to compute the adult organism from the genetic information in the egg. The problem may be approached by viewing the egg as containing a program for development, and considering the logical nature of the program by treating cells as automata and ignoring the details of molecular mechanisms. It is suggested that development is essentially a simple process, the cells having a limited repertoire of overt activities and interacting with each other by means of simple signals, and that general principles may be discerned. The complexity lies in the specification of the internal state which may be described in terms of a gene-switching network. Pattern formation is a central feature in development; it is the process whereby states are assigned to the cells according to their position. such that the appropriate type of cytodifferentiation is selected from the repertoire. The morphogenesis of the chick limb is briefly discussed. Genetic networks that account for such features as memory, competence and interpretation of positional information are given. The question of how these component parts are organized into a complete control system for development is posed as a problem for future study."}
{"id": "PMID:1088944", "title": "Growth and death of diploid and transformed human fibroblasts.", "content": "Three possible explanations are presented for the differences in growth potential between human diploid fibroblasts of finite life-span and permanent transformed lines: 1) Only diploid cells have a molecular clock mechanism which counts cell divisions prior to senescence. Two hypothetical examples of such mechanisms are described; however, the available evidence argues against a clock mechanism for aging in fibroblasts. 2) Cells become committed with a given probability to a slow buildup in protein errors, which leads after many divisions to a lethal error catastrophe. It can be shown that speeding up the rate at which the error catastrophe develops, as may occur in transformed cells, can convert a population of finite life-span to one with infinite growth. 3) The growth rate of diploid cells may not depend on the limiting concentration of any one protein. If so, cells with a low level of errors will not have a reduced generation time, and there will be no selection against them. On the other hand the uncontrolled growth of transformed cells may be reduced in rate by the presence of faulty proteins, so that there is continuous selection for those with the fewest errors. Finally, the analogous problem of the mortality of somatic cells and the immortality of the germ line is also briefly discussed.", "contents": "Growth and death of diploid and transformed human fibroblasts. Three possible explanations are presented for the differences in growth potential between human diploid fibroblasts of finite life-span and permanent transformed lines: 1) Only diploid cells have a molecular clock mechanism which counts cell divisions prior to senescence. Two hypothetical examples of such mechanisms are described; however, the available evidence argues against a clock mechanism for aging in fibroblasts. 2) Cells become committed with a given probability to a slow buildup in protein errors, which leads after many divisions to a lethal error catastrophe. It can be shown that speeding up the rate at which the error catastrophe develops, as may occur in transformed cells, can convert a population of finite life-span to one with infinite growth. 3) The growth rate of diploid cells may not depend on the limiting concentration of any one protein. If so, cells with a low level of errors will not have a reduced generation time, and there will be no selection against them. On the other hand the uncontrolled growth of transformed cells may be reduced in rate by the presence of faulty proteins, so that there is continuous selection for those with the fewest errors. Finally, the analogous problem of the mortality of somatic cells and the immortality of the germ line is also briefly discussed."}
{"id": "PMID:1088945", "title": "Pathological implications of cell aging in vitro.", "content": "The replicative capacity of cultured human fibroblasts is discussed in relation to three areas, diabetes mellitus, expression of HL-A antigens, and interactions with polymerizing fibrin. The replicative capacity of cells is dimished in diabetes mellitus and certain related disorders such as progeria and Werner's syndrome, all of which feature accelerated aging. Expression of HL-A antigens is reduced in progeria fibroblasts compared to normal cultures at corresponding stages of passage. Normal cells show more subtle alteration during aging in vitro probably related to clonal heterogeneity and/or selection within mass cultures. Early-passage fibroblasts interact rapidly with polymerizing fibrin to form a mature clot which is then retracted by a process dependent on cellular integrity and active metabolism. Late-passage cultures are less active in both parameters as are fibroblasts from a subject with progeria. These observations, in total, may relate to altered self-recognition and certain autoimmune concomitants of aging in vivo. They may also help to explain impaired wound healing and increased predisposition to atherothrombosis in aging and diabetic individuals. This system of cultured human fibroblasts should serve as an excellent model to investigate the cellular and molecular basis of diabetes mellitus, aging and related pathology.", "contents": "Pathological implications of cell aging in vitro. The replicative capacity of cultured human fibroblasts is discussed in relation to three areas, diabetes mellitus, expression of HL-A antigens, and interactions with polymerizing fibrin. The replicative capacity of cells is dimished in diabetes mellitus and certain related disorders such as progeria and Werner's syndrome, all of which feature accelerated aging. Expression of HL-A antigens is reduced in progeria fibroblasts compared to normal cultures at corresponding stages of passage. Normal cells show more subtle alteration during aging in vitro probably related to clonal heterogeneity and/or selection within mass cultures. Early-passage fibroblasts interact rapidly with polymerizing fibrin to form a mature clot which is then retracted by a process dependent on cellular integrity and active metabolism. Late-passage cultures are less active in both parameters as are fibroblasts from a subject with progeria. These observations, in total, may relate to altered self-recognition and certain autoimmune concomitants of aging in vivo. They may also help to explain impaired wound healing and increased predisposition to atherothrombosis in aging and diabetic individuals. This system of cultured human fibroblasts should serve as an excellent model to investigate the cellular and molecular basis of diabetes mellitus, aging and related pathology."}
{"id": "PMID:1088946", "title": "Hayflick's hypothesis: an approach to in vivo testing.", "content": "The experimental evidence relating to the hypothesis of finite cellular life is reviewed. It is emphasized that even if somatic cell production were limited its total potential would have to be vast to provide for extensive cellular regeneration. The actual limit of reproductive cell life would therefore not likely be reached in a normal life-span. It is proposed to test the hypothesis by deliberate exhaustion of stem-cell reserve, and iron-55 cytocide is described as an experimental system that might be applicable.", "contents": "Hayflick's hypothesis: an approach to in vivo testing. The experimental evidence relating to the hypothesis of finite cellular life is reviewed. It is emphasized that even if somatic cell production were limited its total potential would have to be vast to provide for extensive cellular regeneration. The actual limit of reproductive cell life would therefore not likely be reached in a normal life-span. It is proposed to test the hypothesis by deliberate exhaustion of stem-cell reserve, and iron-55 cytocide is described as an experimental system that might be applicable."}
{"id": "PMID:1088947", "title": "Current theories of biological aging.", "content": "Several lines of evidence have led to the notion that biological aging occurs as a result of changes in the information-containing molecules either at the genetic or epigenetic level. The error theory, the redundant message theory, the codon restriction theory, and the transcriptional event theory represent the major current conceptualizations of biological aging as held by most gerontologists. The finding that cultured normal human and animal cells undergo a finite number of population doublings in vitro has provided new insights into age changes at the cellular level. The number of mitotic events that cultured normal animal cells can undergo appears to be inversely related to the age of the donor. A direct proportionality exists, however, between the mean maximum life-span of a species and the number of population doublings that their cultured embryonic cells will undergo. The several biochemical decrements known to occur prior to the cessation of mitotic activity in vitro are thought to herald those manifestations of senescence seen in the whole animal. Yet to be explained is how those cell classes such as the germ plasm and continuously propagable cancer cells escape from the inevitability of biological aging.", "contents": "Current theories of biological aging. Several lines of evidence have led to the notion that biological aging occurs as a result of changes in the information-containing molecules either at the genetic or epigenetic level. The error theory, the redundant message theory, the codon restriction theory, and the transcriptional event theory represent the major current conceptualizations of biological aging as held by most gerontologists. The finding that cultured normal human and animal cells undergo a finite number of population doublings in vitro has provided new insights into age changes at the cellular level. The number of mitotic events that cultured normal animal cells can undergo appears to be inversely related to the age of the donor. A direct proportionality exists, however, between the mean maximum life-span of a species and the number of population doublings that their cultured embryonic cells will undergo. The several biochemical decrements known to occur prior to the cessation of mitotic activity in vitro are thought to herald those manifestations of senescence seen in the whole animal. Yet to be explained is how those cell classes such as the germ plasm and continuously propagable cancer cells escape from the inevitability of biological aging."}
{"id": "PMID:1088951", "title": "Haloperidol parenterally for treatment of vomiting and nausea from gastrointestinal disorders in a group of geriatric patients: double-blind, placebo-controlled study.", "content": "Twenty-eight geriatric residents of a nursing home participated in a double-blind study to compare the 12-hour therapeutic effectiveness of a single intramuscular injection (1.0 mg) of haloperidol with that of placebo for the relief of vomiting and nausea due to gastrointestinal disorders. Significantly fewer episodes of vomiting occurred in the haloperidol group than in the placebo group. Nausea also was less frequent in the haloperidol group. After four hours, symptoms recurred much more often in the placebo group. Global evaluations showed that a significantly greater number of haloperidol patients improved markedly than did those given placebo. There were no clinically significant changes in vital signs throughout the study in the haloperidol group. In 1 placebo patient the pulse rate was significantly increased; otherwise no adverse reactions were reported for this group. Thus, in a nursing-home population of geriatric patients who experienced vomiting and nausea due to gastrointestinal disorders, haloperidol administered parenterally proved to be a safe and highly effective antiemetic agent.", "contents": "Haloperidol parenterally for treatment of vomiting and nausea from gastrointestinal disorders in a group of geriatric patients: double-blind, placebo-controlled study. Twenty-eight geriatric residents of a nursing home participated in a double-blind study to compare the 12-hour therapeutic effectiveness of a single intramuscular injection (1.0 mg) of haloperidol with that of placebo for the relief of vomiting and nausea due to gastrointestinal disorders. Significantly fewer episodes of vomiting occurred in the haloperidol group than in the placebo group. Nausea also was less frequent in the haloperidol group. After four hours, symptoms recurred much more often in the placebo group. Global evaluations showed that a significantly greater number of haloperidol patients improved markedly than did those given placebo. There were no clinically significant changes in vital signs throughout the study in the haloperidol group. In 1 placebo patient the pulse rate was significantly increased; otherwise no adverse reactions were reported for this group. Thus, in a nursing-home population of geriatric patients who experienced vomiting and nausea due to gastrointestinal disorders, haloperidol administered parenterally proved to be a safe and highly effective antiemetic agent."}
{"id": "PMID:1088981", "title": "Effect of postmortem tissue fixation on tooth mobility and pocket depth in human beings.", "content": "With the exception of dry skulls, postmortem human material is rarely used in the study of periodontal disease. If the relationship between clinical periodontal measurements, such as mobility and pocket depth, were known antemortem versus postmortem, a new source of information would be available. In this study, such measurements were performed on a patient whose mandible was resected because of a malignant condition. Mobility was found to have decreased; pocket depths initially increased, and then returned to nearly preoperative levels.", "contents": "Effect of postmortem tissue fixation on tooth mobility and pocket depth in human beings. With the exception of dry skulls, postmortem human material is rarely used in the study of periodontal disease. If the relationship between clinical periodontal measurements, such as mobility and pocket depth, were known antemortem versus postmortem, a new source of information would be available. In this study, such measurements were performed on a patient whose mandible was resected because of a malignant condition. Mobility was found to have decreased; pocket depths initially increased, and then returned to nearly preoperative levels."}
{"id": "PMID:1088989", "title": "The cooling, analgesic, and rewarming effects of ice massage on localized skin.", "content": "The study was designed to produce objective data on the rate of local cooling following the ice massage technique of cold application and the rate of rewarming following termination of the ice application. More importantly, the onset of skin analgesia and its duration were evaluated. Sixteen normal subjects participated in four testing sessions, two of which were control session. A thermistor unit with probes monitored skin temperature and a standardized pain-evoking stimulus was used to verify analgesia. Analgesia was elicited by the ice massage only after the localized region had been cooled to, and maintained below, 13.6 degrees Celsius. The rate of cooling for the localized skin was more rapid than the rate of rewarming. The ten-minute ice massage was an effective procedure in achieving analgesia and, thus, may be sufficient to permit therapeutic exercise of selective painful conditions during, and for approximately three minutes immediately following, application.", "contents": "The cooling, analgesic, and rewarming effects of ice massage on localized skin. The study was designed to produce objective data on the rate of local cooling following the ice massage technique of cold application and the rate of rewarming following termination of the ice application. More importantly, the onset of skin analgesia and its duration were evaluated. Sixteen normal subjects participated in four testing sessions, two of which were control session. A thermistor unit with probes monitored skin temperature and a standardized pain-evoking stimulus was used to verify analgesia. Analgesia was elicited by the ice massage only after the localized region had been cooled to, and maintained below, 13.6 degrees Celsius. The rate of cooling for the localized skin was more rapid than the rate of rewarming. The ten-minute ice massage was an effective procedure in achieving analgesia and, thus, may be sufficient to permit therapeutic exercise of selective painful conditions during, and for approximately three minutes immediately following, application."}
{"id": "PMID:1088991", "title": "Effect of alterations in extracellular fluid volume on segmental sodium transport.", "content": "This review focuses on the segmental handling of sodium during alterations in extracellular fluid volume. There is abundant evidence that proximal tubular sodium reabsorption is inhibited by Ringer loading but there are also unequivocal data demonstrating that inhibition of a more distal nephron segment is required for a maximal natriuretic response. From the evidence at hand presently, it would seem that, of the distal nephron segments, only the collecting duct is inhibited by expansion of the extracellular volume per se.", "contents": "Effect of alterations in extracellular fluid volume on segmental sodium transport. This review focuses on the segmental handling of sodium during alterations in extracellular fluid volume. There is abundant evidence that proximal tubular sodium reabsorption is inhibited by Ringer loading but there are also unequivocal data demonstrating that inhibition of a more distal nephron segment is required for a maximal natriuretic response. From the evidence at hand presently, it would seem that, of the distal nephron segments, only the collecting duct is inhibited by expansion of the extracellular volume per se."}
{"id": "PMID:1088992", "title": "Control of the ductus arteriosus.", "content": "In the fetus the major function of the ductus arteriosus is to divert flow away from the lungs toward the placenta, therby avoiding unnecessary circulation through the lungs and reducing the work load of the heart. Constriction of the ductus arteriosus is produced by several mechanisms that may interact with one another in producing normal postnatal closure of the ductus arteriosus. The major mechanism is the constrictor effect of an increased O2 environment. This may directly affect the ductus arteriosus muscle or may work through the release of an intermediary substance. Other vasoactive substance circulating in the blood after birth may also play some role in constriction of the ductus arteriosus. There are major species differences in the degree and type of response to the various stimuli and the exact mechanisms ultimately responsible for closure are not yet fully understood.", "contents": "Control of the ductus arteriosus. In the fetus the major function of the ductus arteriosus is to divert flow away from the lungs toward the placenta, therby avoiding unnecessary circulation through the lungs and reducing the work load of the heart. Constriction of the ductus arteriosus is produced by several mechanisms that may interact with one another in producing normal postnatal closure of the ductus arteriosus. The major mechanism is the constrictor effect of an increased O2 environment. This may directly affect the ductus arteriosus muscle or may work through the release of an intermediary substance. Other vasoactive substance circulating in the blood after birth may also play some role in constriction of the ductus arteriosus. There are major species differences in the degree and type of response to the various stimuli and the exact mechanisms ultimately responsible for closure are not yet fully understood."}
{"id": "PMID:1088997", "title": "Physiology of chemotaxis and random motility.", "content": "The following aspects of random and directional migration of leukocytes have been considered: (1) basic cellular mechanisms involved in leukocyte locomotion; (2) the mechanisms of formation and the nature of various factors such as cytotaxins, cytotaxin-inactivators and inhibitors participating in the regulation of the chemotactic response, and (3) the relationship between chemotaxis and leukocyte emigration. The need for adequate techniques for measuring chemotaxis has been emphasized with special reference to clinical investigation.", "contents": "Physiology of chemotaxis and random motility. The following aspects of random and directional migration of leukocytes have been considered: (1) basic cellular mechanisms involved in leukocyte locomotion; (2) the mechanisms of formation and the nature of various factors such as cytotaxins, cytotaxin-inactivators and inhibitors participating in the regulation of the chemotactic response, and (3) the relationship between chemotaxis and leukocyte emigration. The need for adequate techniques for measuring chemotaxis has been emphasized with special reference to clinical investigation."}
{"id": "PMID:1089000", "title": "Homotransplant renal artery stenosis.", "content": "Transplant renal artery stenosis occurred in 12 of 101 consecutive kidney transplants. Stenoses were all located in the renal artery distal to the anastomosis. Two separate forms of stenosis are recognized: angulation and segmental. All transplant patients with severe diastolic hypertension, refractory to medical management, and an audible abdominal bruit should undergo angiography. Surgical correction of the stenosis was accomplished in nine of 12 patients with cure of their hypertension.", "contents": "Homotransplant renal artery stenosis. Transplant renal artery stenosis occurred in 12 of 101 consecutive kidney transplants. Stenoses were all located in the renal artery distal to the anastomosis. Two separate forms of stenosis are recognized: angulation and segmental. All transplant patients with severe diastolic hypertension, refractory to medical management, and an audible abdominal bruit should undergo angiography. Surgical correction of the stenosis was accomplished in nine of 12 patients with cure of their hypertension."}
{"id": "PMID:1089007", "title": "Current status of the treatment of syphilis.", "content": "Penicillin remains the treatment of choice for syphilis, with sustained low blood levels curing virtually all patients having early syphilis and halting disease progression in most patients with symptomatic syphilis. Tetracycline, erythromycin or cephalothin yields similar cure rates for patients with early syphilis who are allergic to penicillin. The efficacy of non-penicillin regimens for the treatment of late syphilis is uncertain. Results of Venereal Disease Research Laboratory (VDRL) or other reagin tests should become negative or remain at very low titer following adequate therapy, although results of Treponema pallidum immobilization (TPI) and fluorescent treponemal antibody-absorbed (FTA-ABS) tests often remain positive.", "contents": "Current status of the treatment of syphilis. Penicillin remains the treatment of choice for syphilis, with sustained low blood levels curing virtually all patients having early syphilis and halting disease progression in most patients with symptomatic syphilis. Tetracycline, erythromycin or cephalothin yields similar cure rates for patients with early syphilis who are allergic to penicillin. The efficacy of non-penicillin regimens for the treatment of late syphilis is uncertain. Results of Venereal Disease Research Laboratory (VDRL) or other reagin tests should become negative or remain at very low titer following adequate therapy, although results of Treponema pallidum immobilization (TPI) and fluorescent treponemal antibody-absorbed (FTA-ABS) tests often remain positive."}
{"id": "PMID:1089011", "title": "The role of the kidney in spontaneous hypertension.", "content": "There is direct and indirect evidence that the kidneys are involved in the onset of hypertension in spontaneously hypertensive animals. In the Dahl strain, rather convincing evidence exists for a primary, inherent renal defect that is worsened by high dietary salt. In the Okamoto and New Zealand strains, an intrinsic defect may be provoked by increased sympathetic nerve activity. Similarities between all of these strains and Goldblatt hypertension suggest a fluid volume abnormality, but the gradual onset of elevated pressure and continuing growth during development of hypertension may obscure critical volume changes. Theoretically, arterial pressure, somewhat independent of intermediate steps, will reach the level which is dictated by renal function as being necessary for the maintenance of salt and water homeostasis. While widespread use of different spontaneously hypertensive strains may currently be complicating our understanding of the intermediate steps, studies of dissimilar strains should, in time, enhance our understanding of the many different facets of long-term blood pressure control.", "contents": "The role of the kidney in spontaneous hypertension. There is direct and indirect evidence that the kidneys are involved in the onset of hypertension in spontaneously hypertensive animals. In the Dahl strain, rather convincing evidence exists for a primary, inherent renal defect that is worsened by high dietary salt. In the Okamoto and New Zealand strains, an intrinsic defect may be provoked by increased sympathetic nerve activity. Similarities between all of these strains and Goldblatt hypertension suggest a fluid volume abnormality, but the gradual onset of elevated pressure and continuing growth during development of hypertension may obscure critical volume changes. Theoretically, arterial pressure, somewhat independent of intermediate steps, will reach the level which is dictated by renal function as being necessary for the maintenance of salt and water homeostasis. While widespread use of different spontaneously hypertensive strains may currently be complicating our understanding of the intermediate steps, studies of dissimilar strains should, in time, enhance our understanding of the many different facets of long-term blood pressure control."}
{"id": "PMID:1089012", "title": "Interesting aspects of geriatric cardiology.", "content": "Geriatric cardiology requires special knowledge and experience. It is not possible to extrapolate directly experience obtained with young patients to old people. Because of the multiple illnesses, many serious, in the elderly cardiac patients, it is imperative for the cardiologist to be, first of all, a master internist at all times. Old patients with their multiple illnesses are also sensitive to drugs, including digitalis and diuretics. There is a need to train more physicians in geriatric cardiology in order to offer the old patient the best of care since so many old people are living today. There is also a need to learn the effects of the aging process itself on the human heart. Such studies should command priorities in financial and other forms of support.", "contents": "Interesting aspects of geriatric cardiology. Geriatric cardiology requires special knowledge and experience. It is not possible to extrapolate directly experience obtained with young patients to old people. Because of the multiple illnesses, many serious, in the elderly cardiac patients, it is imperative for the cardiologist to be, first of all, a master internist at all times. Old patients with their multiple illnesses are also sensitive to drugs, including digitalis and diuretics. There is a need to train more physicians in geriatric cardiology in order to offer the old patient the best of care since so many old people are living today. There is also a need to learn the effects of the aging process itself on the human heart. Such studies should command priorities in financial and other forms of support."}
{"id": "PMID:1089014", "title": "Use of chromium-adenosine triphosphate and lyxose to elucidate the kinetic mechanism and coordination state of the nucleotide substrate for yeast hexokinase.", "content": "When initial velocities are measured with yeast hexokinase at pH 7, 17 degrees, the inert coordination complex chromium-ATP is competitive vs. MgATP and noncompetitive with glucose, with a dissociation constant of 4-6 muM in either the presence or absence of glucose. These patterns confirm a random kinetic mechanism for this enzyme. With CrATP present, however, the reaction slows down over the first several minutes to a much slower rate, suggesting tighter binding of CrATP with time. When CrATP, MgATP, and D-lyxose are preincubated with the enzyme for 10 min and the reaction started by addition of excess glucose, the dissociation constand of CrATP in now 0.13 muM and the reaction is linear with time. When glucose, CrATP, and enzyme are incubated together and then placed on a Sephadex column, 1 mol each of CrATP and glucose per active center is tightly bound to the enzyme, thus providing a simple and precise method of determining the concentration of active sites. This tight complex, after denaturation with acid, releases 25% free glucose and 75% of a chromium complex containing both ADP and sugar-6-P. CrADP-glucose-6-P is also slowly released from the enzyme during incubation, so that CrATP is actually a very slow substrate. Binding of CrATP with the formation of CrADP-sugar-6-P complexes is also induced by mannose, fructose, glucosamine, 2,5-anhydro-D-glucitol, 2,5-anhydro-D-mannose, and 2,5-anhydro-D-mannitol, while glucose-6-P, 6-deoxyglucose, and lyxose also induce tight binding of CrATP. With excess enzyme, only 25% of CrATP is bound, and the rest does not inhibit the hexokinase reaction. Since bidentate Cr(NH3)4ATP and monodentate CrADP also display inhibition which is tighter with time, but since bidentate CrADP is a poor inhibitor, the actural substrates in the hexokinase reaction appear to be beta, gamma-bidentate MgATP and beta-monodentate MgADP. Tighter inhibition by Cr-8-BrATP than by CrATP suggests that ATP ASSUMES THE SYN CONFORMATION ON THE ENZYME. The substrate inhibition by MgATP induced by the presence of lyxose is shown to be competitive vs. glucose and partial, and, together with other data available, to suggest a kinetic mechanism that is random, but where (1) the rate constant for release of glucose from E-glucose is equal to Vmax, and that for release of glucose from central complexes is less than Vmas; (2) the majority of the reaction flux when both substrates are present at Km levels goes through the path with glucose adding before MgATP, but where at physiological levels the flux through the two paths is more equal. Contd.", "contents": "Use of chromium-adenosine triphosphate and lyxose to elucidate the kinetic mechanism and coordination state of the nucleotide substrate for yeast hexokinase. When initial velocities are measured with yeast hexokinase at pH 7, 17 degrees, the inert coordination complex chromium-ATP is competitive vs. MgATP and noncompetitive with glucose, with a dissociation constant of 4-6 muM in either the presence or absence of glucose. These patterns confirm a random kinetic mechanism for this enzyme. With CrATP present, however, the reaction slows down over the first several minutes to a much slower rate, suggesting tighter binding of CrATP with time. When CrATP, MgATP, and D-lyxose are preincubated with the enzyme for 10 min and the reaction started by addition of excess glucose, the dissociation constand of CrATP in now 0.13 muM and the reaction is linear with time. When glucose, CrATP, and enzyme are incubated together and then placed on a Sephadex column, 1 mol each of CrATP and glucose per active center is tightly bound to the enzyme, thus providing a simple and precise method of determining the concentration of active sites. This tight complex, after denaturation with acid, releases 25% free glucose and 75% of a chromium complex containing both ADP and sugar-6-P. CrADP-glucose-6-P is also slowly released from the enzyme during incubation, so that CrATP is actually a very slow substrate. Binding of CrATP with the formation of CrADP-sugar-6-P complexes is also induced by mannose, fructose, glucosamine, 2,5-anhydro-D-glucitol, 2,5-anhydro-D-mannose, and 2,5-anhydro-D-mannitol, while glucose-6-P, 6-deoxyglucose, and lyxose also induce tight binding of CrATP. With excess enzyme, only 25% of CrATP is bound, and the rest does not inhibit the hexokinase reaction. Since bidentate Cr(NH3)4ATP and monodentate CrADP also display inhibition which is tighter with time, but since bidentate CrADP is a poor inhibitor, the actural substrates in the hexokinase reaction appear to be beta, gamma-bidentate MgATP and beta-monodentate MgADP. Tighter inhibition by Cr-8-BrATP than by CrATP suggests that ATP ASSUMES THE SYN CONFORMATION ON THE ENZYME. The substrate inhibition by MgATP induced by the presence of lyxose is shown to be competitive vs. glucose and partial, and, together with other data available, to suggest a kinetic mechanism that is random, but where (1) the rate constant for release of glucose from E-glucose is equal to Vmax, and that for release of glucose from central complexes is less than Vmas; (2) the majority of the reaction flux when both substrates are present at Km levels goes through the path with glucose adding before MgATP, but where at physiological levels the flux through the two paths is more equal. Contd."}
{"id": "PMID:1089017", "title": "Past presidents I have known.", "content": "This paper is an account of the accomplishments of some of the early past presidents of the Medical Library Association known personally to the author in his career as a medical librarian. It demonstrates the qualities that made these librarians leaders of our profession and also indicates their personal attributes that contributed to the advancement of medical librarianship. It is hoped that the historical presentation of some of the giants of our profession will inspire present and future presidents and other medical librarians with an understanding of some of the qualities necessary to the continuing success of our profession. Sir William Osler, who was a great believer in libraries and librarians and himself a Past President of MLA, summed up four qualities in his advice to medical students equally applicable to past and present leadership in the library profession-(1) the art of detachment, (2) the virtue of method, (3) the quality of thoroughness, (4) the grace of humility (Osler, Sir William. Teacher and Student. In his Aequanimitas: with Other Addresses to Medical Students. 3d ed. Philadelphia, Blakiston Company, 1904. p. 27-31.). It is thought that our past presidents possessed all of these qualities.", "contents": "Past presidents I have known. This paper is an account of the accomplishments of some of the early past presidents of the Medical Library Association known personally to the author in his career as a medical librarian. It demonstrates the qualities that made these librarians leaders of our profession and also indicates their personal attributes that contributed to the advancement of medical librarianship. It is hoped that the historical presentation of some of the giants of our profession will inspire present and future presidents and other medical librarians with an understanding of some of the qualities necessary to the continuing success of our profession. Sir William Osler, who was a great believer in libraries and librarians and himself a Past President of MLA, summed up four qualities in his advice to medical students equally applicable to past and present leadership in the library profession-(1) the art of detachment, (2) the virtue of method, (3) the quality of thoroughness, (4) the grace of humility (Osler, Sir William. Teacher and Student. In his Aequanimitas: with Other Addresses to Medical Students. 3d ed. Philadelphia, Blakiston Company, 1904. p. 27-31.). It is thought that our past presidents possessed all of these qualities."}
{"id": "PMID:1089034", "title": "Death in amphetamine users: causes and rates.", "content": "The world medical literature contains 43 reports of deaths associated with amphetamines in a 35-year period. These included seven cerebrovascular accidents, six sudden cardiac deaths, three cases of hyperpyrexia, eight poisonings of uncertain mechanism and seven cases of medical complications of intravenous injection; the remainder were of uncertain cause. In contrast, in Ontario alone, in 1972 and 1973 there were 26 deaths in amphetamine users, of which 16 were due to accident suicide or homicide. Of the remaining cases, two were cardiac, two hepatic and the rest were mixed drug overdose. Pulmonary granulomata, subacute hepatitis and other lesions resulting from intravenous drug use were common findings at autopsy. On the basis of the estimated number of regular users of intravenous amphetamine in Ontario, the mortality rate in such users is at least four times as high as in the general population of the same age, and is comparable to that in alcoholics and heroin addicts. However, the absolute number of alcohol-related deaths is far greater than the number of deaths in amphetamine or heroin users.", "contents": "Death in amphetamine users: causes and rates. The world medical literature contains 43 reports of deaths associated with amphetamines in a 35-year period. These included seven cerebrovascular accidents, six sudden cardiac deaths, three cases of hyperpyrexia, eight poisonings of uncertain mechanism and seven cases of medical complications of intravenous injection; the remainder were of uncertain cause. In contrast, in Ontario alone, in 1972 and 1973 there were 26 deaths in amphetamine users, of which 16 were due to accident suicide or homicide. Of the remaining cases, two were cardiac, two hepatic and the rest were mixed drug overdose. Pulmonary granulomata, subacute hepatitis and other lesions resulting from intravenous drug use were common findings at autopsy. On the basis of the estimated number of regular users of intravenous amphetamine in Ontario, the mortality rate in such users is at least four times as high as in the general population of the same age, and is comparable to that in alcoholics and heroin addicts. However, the absolute number of alcohol-related deaths is far greater than the number of deaths in amphetamine or heroin users."}
{"id": "PMID:1089036", "title": "Surface immunoglobulin positive lymphocytes in human breast cancer tissue and homolateral axillary lymph nodes.", "content": "Surface immunoglobulins were determined on human lymph node lymphocytes by the use of immunofluorescence technique in 59 breast cancer patients undergoing radical mastectomy. In 10 of these cases, lymphocyte surface immunoglobulins were also studied on lymphocytes infiltrating the primary cancer mass. The most outstanding finding was a difference between the IgM lymphocyte populations in the lymph nodes of patients with and without lymph node metastases. When cancer tissue was present in one or more lymph nodes, the tumor-free as well as the tumor-positive nodes showed a higher percentage of IgM positive lymphocytes than did lymph nodes from patients without nodal metastases. The greatest difference was found when IgM lymphocytes from tumor-bearing lymph nodes were compared with those from the lymph nodes of patients without nodal metastases (p is less than .005). The lymphocyte populations infiltrating 5 of the 10 primary cancer masses studied showed no surface immunoglobulins; in the remainder, both IgG and IgM positive lymphocytes were found but in variable proportions. While  the findings are not definitive, this is the first study dealing with the quantitation of immunoglobulin specific lymphocytes in the lymph nodes and tumor tissue of patients with breast cancer.", "contents": "Surface immunoglobulin positive lymphocytes in human breast cancer tissue and homolateral axillary lymph nodes. Surface immunoglobulins were determined on human lymph node lymphocytes by the use of immunofluorescence technique in 59 breast cancer patients undergoing radical mastectomy. In 10 of these cases, lymphocyte surface immunoglobulins were also studied on lymphocytes infiltrating the primary cancer mass. The most outstanding finding was a difference between the IgM lymphocyte populations in the lymph nodes of patients with and without lymph node metastases. When cancer tissue was present in one or more lymph nodes, the tumor-free as well as the tumor-positive nodes showed a higher percentage of IgM positive lymphocytes than did lymph nodes from patients without nodal metastases. The greatest difference was found when IgM lymphocytes from tumor-bearing lymph nodes were compared with those from the lymph nodes of patients without nodal metastases (p is less than .005). The lymphocyte populations infiltrating 5 of the 10 primary cancer masses studied showed no surface immunoglobulins; in the remainder, both IgG and IgM positive lymphocytes were found but in variable proportions. While  the findings are not definitive, this is the first study dealing with the quantitation of immunoglobulin specific lymphocytes in the lymph nodes and tumor tissue of patients with breast cancer."}
{"id": "PMID:1089037", "title": "Nodular lymphoma: an ultrastructural study of its relationship to germinal centers and a correlation of light and electron microscopic findings.", "content": "This study describes the ultrastructural findings in 16 cases of nodular lymphoma, and compares these with normal germinal centers in order to determine the possible germinal center origin of the lymphomas. Long branching desmosome-associated dendritic reticulum cells, characteristic of germinal centers, were found in all 16 cases of nodular lymphoma. Desmosomes were observed only between dendritic cells and not between lymphoid cells, and they were seen in only 1 of 7 cases of diffuse lymphoma. Cells comprising the nodular lymphomas share cytologic features, such as nuclear blebs and marked nuclear indentations, with germinal center cells. It appears that nodular lymphomas have a close anatomical relationship to germinal centers, although their actual origin from these sites is not established by this study. They are composed of lymphoid cells; we have no evidence to confirm the view that nodular lymphomas are derived from dendritic cells. Light and electron microscopic findings have been compared in order to establish the nature of large \"histiocyte-like\" cells in the nodular lymphomas. Many of these cells appear to represent transformed lymphocytes rather than histiocytes. They are more numerous in so-called mixed \"histiocytic\" lymphocytic lymphomas than in poorly differentiated lymphocytic lymphomas, which otherwise are ultrastructurally similar. This study casts doubt upon the existence of a mixed lymphocytic and histiocytic lymphoma within the spectrum of the nodular lymphomas, and indicates that a cytologic continuum exists between cells interpreted as poorly differentiated lymphocytes and those heretofore identified as histiocytes.", "contents": "Nodular lymphoma: an ultrastructural study of its relationship to germinal centers and a correlation of light and electron microscopic findings. This study describes the ultrastructural findings in 16 cases of nodular lymphoma, and compares these with normal germinal centers in order to determine the possible germinal center origin of the lymphomas. Long branching desmosome-associated dendritic reticulum cells, characteristic of germinal centers, were found in all 16 cases of nodular lymphoma. Desmosomes were observed only between dendritic cells and not between lymphoid cells, and they were seen in only 1 of 7 cases of diffuse lymphoma. Cells comprising the nodular lymphomas share cytologic features, such as nuclear blebs and marked nuclear indentations, with germinal center cells. It appears that nodular lymphomas have a close anatomical relationship to germinal centers, although their actual origin from these sites is not established by this study. They are composed of lymphoid cells; we have no evidence to confirm the view that nodular lymphomas are derived from dendritic cells. Light and electron microscopic findings have been compared in order to establish the nature of large \"histiocyte-like\" cells in the nodular lymphomas. Many of these cells appear to represent transformed lymphocytes rather than histiocytes. They are more numerous in so-called mixed \"histiocytic\" lymphocytic lymphomas than in poorly differentiated lymphocytic lymphomas, which otherwise are ultrastructurally similar. This study casts doubt upon the existence of a mixed lymphocytic and histiocytic lymphoma within the spectrum of the nodular lymphomas, and indicates that a cytologic continuum exists between cells interpreted as poorly differentiated lymphocytes and those heretofore identified as histiocytes."}
{"id": "PMID:1089038", "title": "Aggressive growth and neoplastic potential of odontogenic cysts: with special reference to central epidermoid and mucoepidermoid carcinomas.", "content": "An aggressive form of odontogenic cyst known as the keratocyst has been reviewed with a report on the findings of 70 new instances. Recurrence after 2 years of followup was found to be 20%. From both a review of the literature and original cases, carcinomatous and ameloblastomatous transformation in odontogenic keratocysts seems to be an extremely rare phenomenon. Alternatively, epidermoid carcinoma and mucoepidermoid carcinoma arising centrally within the jaws are often associated with other types of dental cycts. Review of 36 instances of central epidermoid carcinoma has disclosed that 75% were associated with a cyst lining; the 2-year survival rate was found to be 53%. Central mucoepidermoid carcinoma was associated with a dental cyst or impacted tooth in 48% of the cases, and enjoyed a 100% 2-year and 5-year survival; however, 2 patients were recorded alive with disease at 4 months and 10 years, whereas another patient succumbed to disease 14 years postoperatively.", "contents": "Aggressive growth and neoplastic potential of odontogenic cysts: with special reference to central epidermoid and mucoepidermoid carcinomas. An aggressive form of odontogenic cyst known as the keratocyst has been reviewed with a report on the findings of 70 new instances. Recurrence after 2 years of followup was found to be 20%. From both a review of the literature and original cases, carcinomatous and ameloblastomatous transformation in odontogenic keratocysts seems to be an extremely rare phenomenon. Alternatively, epidermoid carcinoma and mucoepidermoid carcinoma arising centrally within the jaws are often associated with other types of dental cycts. Review of 36 instances of central epidermoid carcinoma has disclosed that 75% were associated with a cyst lining; the 2-year survival rate was found to be 53%. Central mucoepidermoid carcinoma was associated with a dental cyst or impacted tooth in 48% of the cases, and enjoyed a 100% 2-year and 5-year survival; however, 2 patients were recorded alive with disease at 4 months and 10 years, whereas another patient succumbed to disease 14 years postoperatively."}
{"id": "PMID:1089039", "title": "Techniques for inhibiting tumor metastases.", "content": "Of the four major biological mechanisms of cancer spread, hematogenous dissemination is perhaps the most significant, as it usually heralds a fatal outcome for the patient. Recent experimental approaches have shown ways of altering the metastatic process and even totally inhibiting it in some animal models. It appears that these models may be applicable to certain human cancers. To prevent hematogenous metastasis formation the process must be inhibited at any one of four levels: 1) growth of the primary; 2) invasion of vessel walls; 3) release of viable tumor cells; or 4) entrapment and growth in distant organs. Judicious handling of the primary can decrease metastasis by minimizing the shedding of tumor cells. New experimental agents prevent the release of tumor cells from the primary by normalizing the blood vessels of the tumor. Warfarin, heparin, and fibrinolytic agents inhibit the entrapment of circulating tumor cells, presumably by their effect on coagulative mechanisms. A better understanding of the benefits of combined approaches to cancer using chemotherapy, irradiation, and immunotherapy, alone and as adjuncts to surgery, offers new opportunity to study methods of controlling metastatic disease.", "contents": "Techniques for inhibiting tumor metastases. Of the four major biological mechanisms of cancer spread, hematogenous dissemination is perhaps the most significant, as it usually heralds a fatal outcome for the patient. Recent experimental approaches have shown ways of altering the metastatic process and even totally inhibiting it in some animal models. It appears that these models may be applicable to certain human cancers. To prevent hematogenous metastasis formation the process must be inhibited at any one of four levels: 1) growth of the primary; 2) invasion of vessel walls; 3) release of viable tumor cells; or 4) entrapment and growth in distant organs. Judicious handling of the primary can decrease metastasis by minimizing the shedding of tumor cells. New experimental agents prevent the release of tumor cells from the primary by normalizing the blood vessels of the tumor. Warfarin, heparin, and fibrinolytic agents inhibit the entrapment of circulating tumor cells, presumably by their effect on coagulative mechanisms. A better understanding of the benefits of combined approaches to cancer using chemotherapy, irradiation, and immunotherapy, alone and as adjuncts to surgery, offers new opportunity to study methods of controlling metastatic disease."}
{"id": "PMID:1089040", "title": "Combined modality therapy of gynecologic cancer.", "content": "In the treatment of gynecologic malignancies, various combinations of surgery, drugs, and radiation therapy are used with increasing frequency. An examination is made of data dealing with combined modalities in the treatment of tumors of the cervix, endometrium, and ovary. Although the efficacy of such treatments has not been established, they are increasingly popular. Newer studies now being conducted, together with newer drugs employed in combination with radiation therapy and surgery, can result in the benefits which to date have not been realized.", "contents": "Combined modality therapy of gynecologic cancer. In the treatment of gynecologic malignancies, various combinations of surgery, drugs, and radiation therapy are used with increasing frequency. An examination is made of data dealing with combined modalities in the treatment of tumors of the cervix, endometrium, and ovary. Although the efficacy of such treatments has not been established, they are increasingly popular. Newer studies now being conducted, together with newer drugs employed in combination with radiation therapy and surgery, can result in the benefits which to date have not been realized."}
{"id": "PMID:1089041", "title": "Preoperative irradiation and surgery for certain cancers.", "content": "Failure to cure cancer by surgery is caused by inability to remove all local extensions of the lesion or from prior or synchronous dissemination of tumor cells. Laboratory evidence has suggested that preoperative irradiation of the primary tumor can increase cure rates. In the clinical realm, the efficacy of preoperative irradiation appears to vary with specific tumors. Lung cancer shows no improvement in survival. Cancers of the bladder and breast may show enhancement of cure rate. Data indicate that cancer of the rectosigmoid does benefit from preoperative therapy. Adherence to important factors of dosage and timing of operation is necessary to prevent undesirable complications. Randomized clinical trials are needed to establish the efficacy of this combined modality treatment system. This is particularly true since both these modalities can be applied on a wide-scale basis if beneficial effects are conclusively demonstrated.", "contents": "Preoperative irradiation and surgery for certain cancers. Failure to cure cancer by surgery is caused by inability to remove all local extensions of the lesion or from prior or synchronous dissemination of tumor cells. Laboratory evidence has suggested that preoperative irradiation of the primary tumor can increase cure rates. In the clinical realm, the efficacy of preoperative irradiation appears to vary with specific tumors. Lung cancer shows no improvement in survival. Cancers of the bladder and breast may show enhancement of cure rate. Data indicate that cancer of the rectosigmoid does benefit from preoperative therapy. Adherence to important factors of dosage and timing of operation is necessary to prevent undesirable complications. Randomized clinical trials are needed to establish the efficacy of this combined modality treatment system. This is particularly true since both these modalities can be applied on a wide-scale basis if beneficial effects are conclusively demonstrated."}
{"id": "PMID:1089042", "title": "Immune inhibition of allogeneic lymphoma cells in the peritoneal cavity of mice.", "content": "Mice were sensitized with cells of normal spleen, transplantable syngeneic lymphomas, or allogeneic lymphomas differing for alloantigens specified by the major histocompatibility complex. From three to eleven days later, the allograft reactivity of these sensitized and appropriate control mice was evaluated in the peritoneal cavity by the disappearance of injected lymphoma cells or the inhibition of DNA synthesis. For the disappearance test, target cells were labeled with [125-I]-5-ido-2'-deoxyuridine before transfer. For the inhibition test, unlabeled target cells were transferred, but these cells were subsequently exposed to the DNA precursor [125-I]-5-iodo-2'-deoxyuridine. In both procedures, cells were recovered from the peritoneal cavity without killing the hosts to measure retained radioactivity. Both tests were immunogenetically specific in detecting secondary allograft reactions, but the disappearance test was less sensitive. By inhibition of DNA synthesis, it was possible to detect primary and secondary reactions, the latter three to eight days after sensitization. Alloantigens associated with the H-2K-Ir regions of Murine Linkage Group IX were more immunogenic than those associated with the Ss-H-2D-Tla regions in eliciting antilymphoma reactions, and female mice responded better than males. It was concluded that the peritoneal inhibition test is sensitive enough to monitor transplantation immunity in vivo and could be applied to animals bearing tumors in sites other than the peritoneum and undergoing chemotherapy.", "contents": "Immune inhibition of allogeneic lymphoma cells in the peritoneal cavity of mice. Mice were sensitized with cells of normal spleen, transplantable syngeneic lymphomas, or allogeneic lymphomas differing for alloantigens specified by the major histocompatibility complex. From three to eleven days later, the allograft reactivity of these sensitized and appropriate control mice was evaluated in the peritoneal cavity by the disappearance of injected lymphoma cells or the inhibition of DNA synthesis. For the disappearance test, target cells were labeled with [125-I]-5-ido-2'-deoxyuridine before transfer. For the inhibition test, unlabeled target cells were transferred, but these cells were subsequently exposed to the DNA precursor [125-I]-5-iodo-2'-deoxyuridine. In both procedures, cells were recovered from the peritoneal cavity without killing the hosts to measure retained radioactivity. Both tests were immunogenetically specific in detecting secondary allograft reactions, but the disappearance test was less sensitive. By inhibition of DNA synthesis, it was possible to detect primary and secondary reactions, the latter three to eight days after sensitization. Alloantigens associated with the H-2K-Ir regions of Murine Linkage Group IX were more immunogenic than those associated with the Ss-H-2D-Tla regions in eliciting antilymphoma reactions, and female mice responded better than males. It was concluded that the peritoneal inhibition test is sensitive enough to monitor transplantation immunity in vivo and could be applied to animals bearing tumors in sites other than the peritoneum and undergoing chemotherapy."}
{"id": "PMID:1089043", "title": "Stimulation of hemolysin plaque-forming cells by idoxuridine.", "content": "5-Iodo-2'-deoxyuridine markedly stimulates the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells in C3HeB/FeJ and A/J male and female mice. The degree of stimulation is dose dependent over a range of 50 to 200 mg/kg. Stimulation is observed when drug is given on the day of antigen administration, or up to 3 days thereafter, but not when given before antigen administration or 4 days thereafter. The stimulatory effect of IUdR given on day 2 after antigen is still noted on Day 15, but the time of HPFC production is not prolonged beyond that in controls. Both 19S and 7S HPFC are increased, although the effect on the latter is less pronounced. In addition to stimulating production of HPFC in immunized animals, 5-iodo-2'-deoxyuridine increases background HPFC production in nonimmunized mice by 250% when assayed on the day after treatment.", "contents": "Stimulation of hemolysin plaque-forming cells by idoxuridine. 5-Iodo-2'-deoxyuridine markedly stimulates the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells in C3HeB/FeJ and A/J male and female mice. The degree of stimulation is dose dependent over a range of 50 to 200 mg/kg. Stimulation is observed when drug is given on the day of antigen administration, or up to 3 days thereafter, but not when given before antigen administration or 4 days thereafter. The stimulatory effect of IUdR given on day 2 after antigen is still noted on Day 15, but the time of HPFC production is not prolonged beyond that in controls. Both 19S and 7S HPFC are increased, although the effect on the latter is less pronounced. In addition to stimulating production of HPFC in immunized animals, 5-iodo-2'-deoxyuridine increases background HPFC production in nonimmunized mice by 250% when assayed on the day after treatment."}
{"id": "PMID:1089044", "title": "Etiological factors, stages, and the role of the foreign body in foreign body tumorigenesis: a review.", "content": "Attempts were made to analyze the process of foreign body (FB) tumorigenesis and to identify etiologically significant factors by correlating information in the literature and recent experimental data from our labroatory. It appears that the process of FB tumorigenesis is dependent on sequence of specific conditions as expressed by the following criteria: (a) cellular proliferation and tissue infiltration during acute FB reaction; (b) fibrosis of the tissue capsule surrounding the FB; (c) quiescence of the tissue reaction, i. e., dormancy and phagocytic inactivity of FB-attached macrophages; and (d) availability of a FB surface for direct contact with clonal preneoplastic cells. There is no indication that the initial acquisition of neoplastic potential and the determination of specific tumor characteristics are based on direct physical or chemical reaction between cells and the FB. These etiological key events occur presumably in mesenchymal stem cells associated with the microvasculature no later than during the acute stage of FB reaction and certainly long before clonal descendants of these cells are first found in contact with the FB surface. In fact, there is no reason to assume that cells with neoplastic determination may be present in normal tissue prior to the introduction of a FB and that the FB would only create the conditions required for stepwise preneoplastic maturation.", "contents": "Etiological factors, stages, and the role of the foreign body in foreign body tumorigenesis: a review. Attempts were made to analyze the process of foreign body (FB) tumorigenesis and to identify etiologically significant factors by correlating information in the literature and recent experimental data from our labroatory. It appears that the process of FB tumorigenesis is dependent on sequence of specific conditions as expressed by the following criteria: (a) cellular proliferation and tissue infiltration during acute FB reaction; (b) fibrosis of the tissue capsule surrounding the FB; (c) quiescence of the tissue reaction, i. e., dormancy and phagocytic inactivity of FB-attached macrophages; and (d) availability of a FB surface for direct contact with clonal preneoplastic cells. There is no indication that the initial acquisition of neoplastic potential and the determination of specific tumor characteristics are based on direct physical or chemical reaction between cells and the FB. These etiological key events occur presumably in mesenchymal stem cells associated with the microvasculature no later than during the acute stage of FB reaction and certainly long before clonal descendants of these cells are first found in contact with the FB surface. In fact, there is no reason to assume that cells with neoplastic determination may be present in normal tissue prior to the introduction of a FB and that the FB would only create the conditions required for stepwise preneoplastic maturation."}
{"id": "PMID:1089060", "title": "LH-RH-containing neural elements in the rat hypothalamus.", "content": "With the use of rabbit anti-LH-RH serum, a system of delicate nerve fibers and terminals which contain immunoreactive LH-RH can be demonstrated in the median eminence of the rat, corresponding exactly to the tubero-infundibular tract as described earlier with the aid of classical neurophistological methods. The delicate fiber system can be traced to the region of the rectrochiasmatic area and especially to that of the arcuate nucleus but not farther. Nerve cells do not show any immunopositive reaction for LH-RH in the experimental conditions studied. LH-RH positive nerve fibers terminate on or in the immediate vicinity of the capillary loops penetrating the median eminence.", "contents": "LH-RH-containing neural elements in the rat hypothalamus. With the use of rabbit anti-LH-RH serum, a system of delicate nerve fibers and terminals which contain immunoreactive LH-RH can be demonstrated in the median eminence of the rat, corresponding exactly to the tubero-infundibular tract as described earlier with the aid of classical neurophistological methods. The delicate fiber system can be traced to the region of the rectrochiasmatic area and especially to that of the arcuate nucleus but not farther. Nerve cells do not show any immunopositive reaction for LH-RH in the experimental conditions studied. LH-RH positive nerve fibers terminate on or in the immediate vicinity of the capillary loops penetrating the median eminence."}
{"id": "PMID:1089061", "title": "Pineal methoxyindoles: new evidence concerning their function in the control of pineal-mediated changes in the reproductive physiology of male golden hamsters.", "content": "Maintaining adult male golden hamsters in short daily photoperiods (1hr of light and 23hr of darkness daily; LD 1.23) for 12 weeks caused the tests and accessory sex organs to atrophy and also led to significant depressions in pituitary LH and prolactin levels. If hamsters that were kept in LD 1.23 CYCLES RECEIVED WEEKLy subcutanceous implants of either a melanioninbeeswax or a 5-methoxtrypotophol-beeswax pellet (1 mgindole in 24 mg beeswax) the testes and accessory sex organs failed to involute and pituitary LH levels did not drop. Both treatments also retarded the depression in hypophyseal prolactin retarded the depression in hypophyseal prolactin levels. Treatment (twice daily on weekdays and once daily on weekends) with 1.5 mug injections of synthetic LRH (in 0.2 ml 8% gelatin) did not prevent gonadal or accessory organ atrophy while it further depressed hypophyseal LRH treatment, plasma LH titers were significantly elevated. The reproductive organs of hamsters that were moved from short (LD1;I 23) to long (LD 14;10) daily photoperiods regenerated within 8 weeks. This light-induced restoration of the gonads was not prevented or retarded by the weekly implantation of either melantonin-beeswax or 5-methoxytrptophol-beeswax pellets. The results suggest that in the male golden hamster neither melatonin nor 5-methoxtryptophol accounts for the antigonadotrophic activity of the pineal gland.", "contents": "Pineal methoxyindoles: new evidence concerning their function in the control of pineal-mediated changes in the reproductive physiology of male golden hamsters. Maintaining adult male golden hamsters in short daily photoperiods (1hr of light and 23hr of darkness daily; LD 1.23) for 12 weeks caused the tests and accessory sex organs to atrophy and also led to significant depressions in pituitary LH and prolactin levels. If hamsters that were kept in LD 1.23 CYCLES RECEIVED WEEKLy subcutanceous implants of either a melanioninbeeswax or a 5-methoxtrypotophol-beeswax pellet (1 mgindole in 24 mg beeswax) the testes and accessory sex organs failed to involute and pituitary LH levels did not drop. Both treatments also retarded the depression in hypophyseal prolactin retarded the depression in hypophyseal prolactin levels. Treatment (twice daily on weekdays and once daily on weekends) with 1.5 mug injections of synthetic LRH (in 0.2 ml 8% gelatin) did not prevent gonadal or accessory organ atrophy while it further depressed hypophyseal LRH treatment, plasma LH titers were significantly elevated. The reproductive organs of hamsters that were moved from short (LD1;I 23) to long (LD 14;10) daily photoperiods regenerated within 8 weeks. This light-induced restoration of the gonads was not prevented or retarded by the weekly implantation of either melantonin-beeswax or 5-methoxytrptophol-beeswax pellets. The results suggest that in the male golden hamster neither melatonin nor 5-methoxtryptophol accounts for the antigonadotrophic activity of the pineal gland."}
{"id": "PMID:1089062", "title": "Direct action of prostaglandins on the rat pituitary.", "content": "The purpose of this study is to ascertain whether or not prostaglandins (PGs) act directly on the pituitary. In the first experiment, the plasma LH levels were sharply decreased 24 hr after the production of hypothalamic lesions in ovariectomized mature rats. Ten min after the iv injection of PGE1, PGE2 or PGF2alpha, the plasma LH levels increased significantly as compared with the vehicle control. Also, the plasma LH levels were elevated following the iv injection of LH-releasing hormone (LH-RH). In the second experiment, after pretreatment of ovariectomized mature rats with estrogen and progesterone, animals received a SC injection of indomethacin, 2 mg in 0.2 ml of gelatin for 3 days. Twenty-four hr after the last injection, 100 ng of LH-RH were injected into the jugular vein. The response of the pituitary to the LH-RH was significantly lower in the indomethacin-treated rats than in the vehicle control rats. In the 3rd experiment, we tested several PGs for stimulatory effects on LH secretion by injecting them into the anterior pituitary. Following direct microinjection of PGE1 or PGE2 (50 or 100 mug/mul) into the rat pituitary, plasma LH was increased at 10 and 45 min after the injection. However, PGF2alpha at both 50 and 100 mug/5mul, failed to increase the plasma LH levels. In the last experiment, the effect of PGE1, PGE2 or PGF2alpha on in vitro rat anterior pituitary LH release was studied. Addition of PGE1, (2 or 20 mug), PGE2 (0.002 or 0.02 mug) or PGF2alpha (200 mug) to the incubation medium produced a significant increase in LH release into the incubation medium. These observations indicate that sufficient dosages of PGs can also stimulate LH secretion by acting directly on the pituitary.", "contents": "Direct action of prostaglandins on the rat pituitary. The purpose of this study is to ascertain whether or not prostaglandins (PGs) act directly on the pituitary. In the first experiment, the plasma LH levels were sharply decreased 24 hr after the production of hypothalamic lesions in ovariectomized mature rats. Ten min after the iv injection of PGE1, PGE2 or PGF2alpha, the plasma LH levels increased significantly as compared with the vehicle control. Also, the plasma LH levels were elevated following the iv injection of LH-releasing hormone (LH-RH). In the second experiment, after pretreatment of ovariectomized mature rats with estrogen and progesterone, animals received a SC injection of indomethacin, 2 mg in 0.2 ml of gelatin for 3 days. Twenty-four hr after the last injection, 100 ng of LH-RH were injected into the jugular vein. The response of the pituitary to the LH-RH was significantly lower in the indomethacin-treated rats than in the vehicle control rats. In the 3rd experiment, we tested several PGs for stimulatory effects on LH secretion by injecting them into the anterior pituitary. Following direct microinjection of PGE1 or PGE2 (50 or 100 mug/mul) into the rat pituitary, plasma LH was increased at 10 and 45 min after the injection. However, PGF2alpha at both 50 and 100 mug/5mul, failed to increase the plasma LH levels. In the last experiment, the effect of PGE1, PGE2 or PGF2alpha on in vitro rat anterior pituitary LH release was studied. Addition of PGE1, (2 or 20 mug), PGE2 (0.002 or 0.02 mug) or PGF2alpha (200 mug) to the incubation medium produced a significant increase in LH release into the incubation medium. These observations indicate that sufficient dosages of PGs can also stimulate LH secretion by acting directly on the pituitary."}
{"id": "PMID:1089082", "title": "Cirrhosis associated with partial deficiency of alpha-1-antitrypsin: a clinical and autopsy study.", "content": "A 63 year old woman with cryptogenic cirrhosis, ascites, portal hypertension, and intermediate levels of alpha-1-antitrypsin of protease inhibitor SZ phenotype who died of esophageal variceal hemorrhage is described. The partial deficiency of alpha-1-antitrypsin and the diagnosis of cirrhosis were suspected one year prior to death because a needle biopsy of liver showed PAS positive, diastase resistant cytoplasmic bodies within hepatocytes. This report illustrates three previously undescribed features: (1) Heterozygous protease inhibitor SZ phenotype may be associated with coarsely nodular cirrhosis in the older adult. (2) The large intracytoplasmic glycoprotein droplets that are distinctive by light microscopy are probably formalin induced aggregates of submicroscopic flocculent material. (3) In the older patients with aberrant alpha-1-antitrypsin the flocculent material is present not only in the granular endoplasmic reticulum but also in smooth endoplasmic reticulum vesicles and cytolysosomes.", "contents": "Cirrhosis associated with partial deficiency of alpha-1-antitrypsin: a clinical and autopsy study. A 63 year old woman with cryptogenic cirrhosis, ascites, portal hypertension, and intermediate levels of alpha-1-antitrypsin of protease inhibitor SZ phenotype who died of esophageal variceal hemorrhage is described. The partial deficiency of alpha-1-antitrypsin and the diagnosis of cirrhosis were suspected one year prior to death because a needle biopsy of liver showed PAS positive, diastase resistant cytoplasmic bodies within hepatocytes. This report illustrates three previously undescribed features: (1) Heterozygous protease inhibitor SZ phenotype may be associated with coarsely nodular cirrhosis in the older adult. (2) The large intracytoplasmic glycoprotein droplets that are distinctive by light microscopy are probably formalin induced aggregates of submicroscopic flocculent material. (3) In the older patients with aberrant alpha-1-antitrypsin the flocculent material is present not only in the granular endoplasmic reticulum but also in smooth endoplasmic reticulum vesicles and cytolysosomes."}
{"id": "PMID:1089083", "title": "Coxsackie viral infection of human myocardium.", "content": "The myocardiu m of a young patient who had clinically established cardiomyopathy and suspected Coxsackie virus B4 infection was studied. Coxsackie B4 viral antigen was found in the ventricular and atrial myocardium by specific immunofluorescent antibody staining. Histologic examination revealed varying degrees of myocardial damage. Interstitial fibrosis and edema, swelling and deterioration of hypertrophic muscle fibers, connective tissue proliferation, stasis of small coronary blood vessels, atrophy of myocardial fibers with pyknosis of nuclei, and lytic deterioration were observed. Electron microscopic examination showed portions of the Z bands to be either widened or displaced into the sarcomere. Adjacent cell membranes in the region of the intercalated disc in the myocardium of both the ventricle and the atrium were separated, forming large gaps. Morphologic changes were most pronounced in the atrium adjacent to the mitral valve, in which the mitochondria were grossly swollen, and large vesicles were present in the sarcoplasm. The pathologic changes found in the myocardium of all chambers of the heart apparently were due to Coxsackie B4 viral infection.", "contents": "Coxsackie viral infection of human myocardium. The myocardiu m of a young patient who had clinically established cardiomyopathy and suspected Coxsackie virus B4 infection was studied. Coxsackie B4 viral antigen was found in the ventricular and atrial myocardium by specific immunofluorescent antibody staining. Histologic examination revealed varying degrees of myocardial damage. Interstitial fibrosis and edema, swelling and deterioration of hypertrophic muscle fibers, connective tissue proliferation, stasis of small coronary blood vessels, atrophy of myocardial fibers with pyknosis of nuclei, and lytic deterioration were observed. Electron microscopic examination showed portions of the Z bands to be either widened or displaced into the sarcomere. Adjacent cell membranes in the region of the intercalated disc in the myocardium of both the ventricle and the atrium were separated, forming large gaps. Morphologic changes were most pronounced in the atrium adjacent to the mitral valve, in which the mitochondria were grossly swollen, and large vesicles were present in the sarcoplasm. The pathologic changes found in the myocardium of all chambers of the heart apparently were due to Coxsackie B4 viral infection."}
{"id": "PMID:1089084", "title": "Inflammatory bowel disease: the surgical pathology of Crohn's disease and ulcerative colitis.", "content": "Ulcerative colitis and granulomatous colitis are distinct entities, but up to 10 per cent of colectomy specimens remain unclassified. Ulcerative colitis is primarily a mucosal disease, and other changes appear to be secondary to this process. By contrast, Crohn's disease, or granulomatous colitis, involves the whole thickness of the bowel wall. About 20 per cent of the cases of Crohn's disease involve the small and large bowel, while another 20 per cent are restricted to the large bowel. Since granulomatous colitis is a patchy disease, and many of the changes are deep within the bowel wall, rectal biopsy may not be as helpful as in ulcerative colitis. Fully developed granulomas are present in only a small minority of cases, and a diagnostic report of granulomatous colitis may be given in the absence of granulomas. In biopsy material, the differentiation of inflammatory bowel disease from ischemic colitis and pseudomembranous colitis may be difficult. In the absence of specific demonstration of an organism it may also be impossible on rectal biopsy to distinguish amebic or bacillary dysentery from ulcerative colitis. Even by colectomy, 29 of 300 specimens were sufficiently atypical so as not to warrant a label of Crohn's disease, or ulcerative colitis. Cancer of the colon, which is common in ulcerative colitis, is rare in Crohn's disease, but may also represent a definite complication in the latter. Immunologic studies are still confusing, but it is suggested that patients with ulcerative colitis and Crohn's disease may have a state of altered immunologic reactivity.", "contents": "Inflammatory bowel disease: the surgical pathology of Crohn's disease and ulcerative colitis. Ulcerative colitis and granulomatous colitis are distinct entities, but up to 10 per cent of colectomy specimens remain unclassified. Ulcerative colitis is primarily a mucosal disease, and other changes appear to be secondary to this process. By contrast, Crohn's disease, or granulomatous colitis, involves the whole thickness of the bowel wall. About 20 per cent of the cases of Crohn's disease involve the small and large bowel, while another 20 per cent are restricted to the large bowel. Since granulomatous colitis is a patchy disease, and many of the changes are deep within the bowel wall, rectal biopsy may not be as helpful as in ulcerative colitis. Fully developed granulomas are present in only a small minority of cases, and a diagnostic report of granulomatous colitis may be given in the absence of granulomas. In biopsy material, the differentiation of inflammatory bowel disease from ischemic colitis and pseudomembranous colitis may be difficult. In the absence of specific demonstration of an organism it may also be impossible on rectal biopsy to distinguish amebic or bacillary dysentery from ulcerative colitis. Even by colectomy, 29 of 300 specimens were sufficiently atypical so as not to warrant a label of Crohn's disease, or ulcerative colitis. Cancer of the colon, which is common in ulcerative colitis, is rare in Crohn's disease, but may also represent a definite complication in the latter. Immunologic studies are still confusing, but it is suggested that patients with ulcerative colitis and Crohn's disease may have a state of altered immunologic reactivity."}
{"id": "PMID:1089085", "title": "Microcirculation in diabetes mellitus: a study of gingival biopsies.", "content": "Gingival biopsy specimens from 24 diabetic patients, six obese persons, and six controls were studied statistically and morphologically to detect vascular basement membrane changes using a proposed classification of microvessels. Statistically significant basement membrane thickening is seen in all diabetic groups in most true capillaries and vessels proximal to them, especially in terminal arterioles and metarterioles, although the degree and extent of the changes vary among different clinical groups and vessel types. The basement membrane thickening is characterized by increased amorphous, granular, and fibrillary material with occasional scattered collagenous fibrils. Although nonspecific, basement membrane thickening in microvessels at, and especially proximal to, the level of true capillaries is intimately related to diabetes. Gingival biopsy may possibly be used as an adjunct in the diagnosis of diabetes.", "contents": "Microcirculation in diabetes mellitus: a study of gingival biopsies. Gingival biopsy specimens from 24 diabetic patients, six obese persons, and six controls were studied statistically and morphologically to detect vascular basement membrane changes using a proposed classification of microvessels. Statistically significant basement membrane thickening is seen in all diabetic groups in most true capillaries and vessels proximal to them, especially in terminal arterioles and metarterioles, although the degree and extent of the changes vary among different clinical groups and vessel types. The basement membrane thickening is characterized by increased amorphous, granular, and fibrillary material with occasional scattered collagenous fibrils. Although nonspecific, basement membrane thickening in microvessels at, and especially proximal to, the level of true capillaries is intimately related to diabetes. Gingival biopsy may possibly be used as an adjunct in the diagnosis of diabetes."}
{"id": "PMID:1089088", "title": "Growth and transplantation of organ-cultured corneas.", "content": "Calf corneal buttons were grown in medium M199 supplemented with newborn calf serum (xenogeneic to the recipient) or rabbit serum (allongeneic to the recipient) and implanted interlamellarly into rabbit recipients. Implantation of buttons, grown in medium containing calf serum were rejected sooner than noncultured buttons. The rejection was quicker as the grafts were kept longer in culture. However, corneal buttons grown in medium containing rabbit serum showed, in general, a slower rejection process.", "contents": "Growth and transplantation of organ-cultured corneas. Calf corneal buttons were grown in medium M199 supplemented with newborn calf serum (xenogeneic to the recipient) or rabbit serum (allongeneic to the recipient) and implanted interlamellarly into rabbit recipients. Implantation of buttons, grown in medium containing calf serum were rejected sooner than noncultured buttons. The rejection was quicker as the grafts were kept longer in culture. However, corneal buttons grown in medium containing rabbit serum showed, in general, a slower rejection process."}
{"id": "PMID:1089089", "title": "Ciliary ganglion stimulation. I. Effects on aqueous humor inflow and outflow.", "content": "Stimulation of the ciliary ganglion in an enucleated, arterially perfused cat eye preparation produced a sustained increase in aqueous humor formation and an increase in the rate of aqueous humor outflow. The increased aqueous humor formation induced by ciliary ganglion stimulation has been found to be pressure-dependent and therefore suggests that ultrafiltration may be the underlying mechanism of action. No change in capillary permeability of the ciliary body could be demonstrated.", "contents": "Ciliary ganglion stimulation. I. Effects on aqueous humor inflow and outflow. Stimulation of the ciliary ganglion in an enucleated, arterially perfused cat eye preparation produced a sustained increase in aqueous humor formation and an increase in the rate of aqueous humor outflow. The increased aqueous humor formation induced by ciliary ganglion stimulation has been found to be pressure-dependent and therefore suggests that ultrafiltration may be the underlying mechanism of action. No change in capillary permeability of the ciliary body could be demonstrated."}
{"id": "PMID:1089090", "title": "Marijuana smoking and reduced pressure in human eyes: drug action or epiphenomenon?", "content": "Normal pressure within the human eye was reduced after smoking a socially relevant dose of marijuana (12 mg. delta9-9-tetrahydrocannabinol), but only for light to moderate users who experienced a substantial \"high\" and a state of peaceful relaxation from the experimental dose. Analysis suggests an indirect effect of the drug associated with relaxation-a psychophysiologic state that can be produced by drug and nondrug  means.", "contents": "Marijuana smoking and reduced pressure in human eyes: drug action or epiphenomenon? Normal pressure within the human eye was reduced after smoking a socially relevant dose of marijuana (12 mg. delta9-9-tetrahydrocannabinol), but only for light to moderate users who experienced a substantial \"high\" and a state of peaceful relaxation from the experimental dose. Analysis suggests an indirect effect of the drug associated with relaxation-a psychophysiologic state that can be produced by drug and nondrug  means."}
{"id": "PMID:1089095", "title": "Improved corneal storage for penetrating keratoplasties in man.", "content": "Twenty-six of 28 human corneas stored for up to seven days in a simple tissue culture medium (dextran dissolved in tissue culture medium with streptomycin-penicillin mixture) remained clear and thin after keratoplasty. Temperature reversal phenomenon and electron microscopy demonstrated high quality of such tissue after storage. The method is inexpensive, requires limited technical assistance, and provides the surgeon with excellent tissue at regular operation hours.", "contents": "Improved corneal storage for penetrating keratoplasties in man. Twenty-six of 28 human corneas stored for up to seven days in a simple tissue culture medium (dextran dissolved in tissue culture medium with streptomycin-penicillin mixture) remained clear and thin after keratoplasty. Temperature reversal phenomenon and electron microscopy demonstrated high quality of such tissue after storage. The method is inexpensive, requires limited technical assistance, and provides the surgeon with excellent tissue at regular operation hours."}
{"id": "PMID:1089096", "title": "Transvitreal chorioretinal biopsy in the rabbit.", "content": "Transvitreal chorioretinal biopsy was performed in nine Dutch rabbits by introducing a biopsy instrument through a small eye wall incision, passing the instrument across the eye through the vitreous to the biopsy site, and by trephinating and removing from the eye a 1.6-mm diameter specimen of the posterior eye wall. Biopsy was carried out under stereotaxic control by using a micromanipulator especially designed for ophthalmic surgery. Hemostasis was achieved by circumferential mechanical pressure around the biopsy site. The eye wall defect was sealed by a preplaced explant and repaired by fibrovascular ingrowth from the surrounding choroid and episclera. The normal histologic features of the biopsy specimen were preserved, except for separation of the neurosensory retina from the pigmented epithelium. Complications of the procedure included hemorrhage from the incision site, transvitreal strand formation, and lens abrasion. In no instance, however, did these complications result in significant opacification of the optical media. The biopsy technique has potential use as a clinical procedure in the diagnosis of choroidal tumors and other selected disorders of the choroid and retina and as a technique in investigational animal studies.", "contents": "Transvitreal chorioretinal biopsy in the rabbit. Transvitreal chorioretinal biopsy was performed in nine Dutch rabbits by introducing a biopsy instrument through a small eye wall incision, passing the instrument across the eye through the vitreous to the biopsy site, and by trephinating and removing from the eye a 1.6-mm diameter specimen of the posterior eye wall. Biopsy was carried out under stereotaxic control by using a micromanipulator especially designed for ophthalmic surgery. Hemostasis was achieved by circumferential mechanical pressure around the biopsy site. The eye wall defect was sealed by a preplaced explant and repaired by fibrovascular ingrowth from the surrounding choroid and episclera. The normal histologic features of the biopsy specimen were preserved, except for separation of the neurosensory retina from the pigmented epithelium. Complications of the procedure included hemorrhage from the incision site, transvitreal strand formation, and lens abrasion. In no instance, however, did these complications result in significant opacification of the optical media. The biopsy technique has potential use as a clinical procedure in the diagnosis of choroidal tumors and other selected disorders of the choroid and retina and as a technique in investigational animal studies."}
{"id": "PMID:1089097", "title": "Ocular reticulum cell sarcoma.", "content": "In four of eight cases of reticulum cell sarcoma with ocular involvement diagnosis was made by craniotomy when the signs of a brain tumor developed. A fifth case had an isolated intraocular tumor. Of the other three, two were diagnosed by vitreous aspiration and one by cerebrospinal fluid cytology. Ocular reticulum cell sarcoma frequently accompanies or precedes brain involvement. In this form of the disease the ocular lesion is usually a tumor cell infiltrate of the retina. The associated retinochoroiditis leads to marked vitreous clouding and eventual retinal detachment and glaucoma may occur. On the other hand, when intraocular reticulum cell sarcoma occurs in association with the systemic lymph node visceral form of the disease, choroidal involvement is the rule. Vitreous aspiration may confirm the diagnosis at an early stage when clinical and laboratory evaluation reveal no disease outside the globe. The retinal tumor foci are radiosensitive and local radiation, although not curative, may restore visual acuity. Immunosuppressive therapy given in low doses for anti-inflammatory effect may exacerbate the disease.", "contents": "Ocular reticulum cell sarcoma. In four of eight cases of reticulum cell sarcoma with ocular involvement diagnosis was made by craniotomy when the signs of a brain tumor developed. A fifth case had an isolated intraocular tumor. Of the other three, two were diagnosed by vitreous aspiration and one by cerebrospinal fluid cytology. Ocular reticulum cell sarcoma frequently accompanies or precedes brain involvement. In this form of the disease the ocular lesion is usually a tumor cell infiltrate of the retina. The associated retinochoroiditis leads to marked vitreous clouding and eventual retinal detachment and glaucoma may occur. On the other hand, when intraocular reticulum cell sarcoma occurs in association with the systemic lymph node visceral form of the disease, choroidal involvement is the rule. Vitreous aspiration may confirm the diagnosis at an early stage when clinical and laboratory evaluation reveal no disease outside the globe. The retinal tumor foci are radiosensitive and local radiation, although not curative, may restore visual acuity. Immunosuppressive therapy given in low doses for anti-inflammatory effect may exacerbate the disease."}
{"id": "PMID:1089099", "title": "Neutropenia and salmonellosis in hospitalized horses.", "content": "Colitis due to salmonellae was diagnosed in 9 horses following hospitalization for various reasons at the University of Missouri Veterinary Teaching Hospital, from May, 1971, to April, 1972. Diarrhea, fever, and either a neutrophil count of less than or equal to 3,600/cmm or a rapid decline in neutrophil numbers were specific for salmonellosis. The value of hematologic survelillance in hospitalized Equidae was demonstrated in another group of 9 horses with neutropenia, each of which was promptly treated and did not develop colitis. Bacteriologic culturing of fecal samples from 28 clinically normal horses yielded only 2 salmonella isolations, S manhattan in each case. The serotyped salmonellae isolated from the patients with colitis were all S typhimurium, with similar antibiotic resistance patterns.", "contents": "Neutropenia and salmonellosis in hospitalized horses. Colitis due to salmonellae was diagnosed in 9 horses following hospitalization for various reasons at the University of Missouri Veterinary Teaching Hospital, from May, 1971, to April, 1972. Diarrhea, fever, and either a neutrophil count of less than or equal to 3,600/cmm or a rapid decline in neutrophil numbers were specific for salmonellosis. The value of hematologic survelillance in hospitalized Equidae was demonstrated in another group of 9 horses with neutropenia, each of which was promptly treated and did not develop colitis. Bacteriologic culturing of fecal samples from 28 clinically normal horses yielded only 2 salmonella isolations, S manhattan in each case. The serotyped salmonellae isolated from the patients with colitis were all S typhimurium, with similar antibiotic resistance patterns."}
{"id": "PMID:1089100", "title": "Generalized mastocytosis in a foal, resembling urticaria pigmentosa of man.", "content": "A newborn foal with multiple, nodular, cutaneous lesions containing mast cells and with mast cell accumulations in bone marrow was observed for 1 year. Cutaneous nodules grew to several centimeters in size, ulcerated, and regressed. Only a few healed nodules remained when the foal was 1 year old. The disease was considered to be similar to urticaria pigmentosa of man.", "contents": "Generalized mastocytosis in a foal, resembling urticaria pigmentosa of man. A newborn foal with multiple, nodular, cutaneous lesions containing mast cells and with mast cell accumulations in bone marrow was observed for 1 year. Cutaneous nodules grew to several centimeters in size, ulcerated, and regressed. Only a few healed nodules remained when the foal was 1 year old. The disease was considered to be similar to urticaria pigmentosa of man."}
{"id": "PMID:1089102", "title": "Distribution of red and white blood cells in alveolar walls.", "content": "Dog lungs were perfused with blood and rapidly frozen with liquid Freon gas at various pulmonary artery and venous pressures. The numbers of red and white blood cells per mm-2 of alveolar wall were counted in lung sections and, in addition, the proportion (by area) of the wall occupied by the cells was measured by point counting. The number and proportional area of the red blood cells rapidly increased as perfusing pressure was raised. These findings are consistent with earlier observations of capillary recruitment and distension. An unexpected observation was the large number of leukocytes in the capillaries especially at low perfusing pressures. For example when arterial exceeded alveolar pressure by 5 cmH2O (as occurs near the apex of the upright human lung), there were about 5,000 red cells and 4,000 white cells per mm-2 of alveolar wall. As perfusing pressure was increased, the number of leukocytes paradoxically decreased in zone 3 but remained constant in zone 2. Most of the white cells were mononuclear cells. These results suggest that the lung behaves as a mechanical sieve for large cells and that the number of trapped cells depends on the capillary pressure.", "contents": "Distribution of red and white blood cells in alveolar walls. Dog lungs were perfused with blood and rapidly frozen with liquid Freon gas at various pulmonary artery and venous pressures. The numbers of red and white blood cells per mm-2 of alveolar wall were counted in lung sections and, in addition, the proportion (by area) of the wall occupied by the cells was measured by point counting. The number and proportional area of the red blood cells rapidly increased as perfusing pressure was raised. These findings are consistent with earlier observations of capillary recruitment and distension. An unexpected observation was the large number of leukocytes in the capillaries especially at low perfusing pressures. For example when arterial exceeded alveolar pressure by 5 cmH2O (as occurs near the apex of the upright human lung), there were about 5,000 red cells and 4,000 white cells per mm-2 of alveolar wall. As perfusing pressure was increased, the number of leukocytes paradoxically decreased in zone 3 but remained constant in zone 2. Most of the white cells were mononuclear cells. These results suggest that the lung behaves as a mechanical sieve for large cells and that the number of trapped cells depends on the capillary pressure."}
{"id": "PMID:1089103", "title": "Expiratory reserve volume and vital capacity of the lungs during immersion in water.", "content": "The effects of immersion by 5-cm increments on the expiratory reserve volume of the lungs (ERV) and on the vital capacity were studied in the sitting and supine positions. These effects were compared to those produced by continuous negative-pressure breathing when the subjects were in air and were counteracted by positive pressure breathing during immersion. The depth of immersion was also related to definable anatomic landmarks. In the sitting position about one-fourth of the decrease in the ERV was accounted for by the hydrostatic pressure of the water on the abdomen and the remainder by the pressure on the thorax. Immersion to the level of the spinous process of the seventh cervical vertebra was equivalent to 28 cmH2o continuous negative pressure breathing in air. In the supine position, a comparable value was 8 cmH2o. These observations agree well with those of others if differences in the levels of immersion are accounted for.", "contents": "Expiratory reserve volume and vital capacity of the lungs during immersion in water. The effects of immersion by 5-cm increments on the expiratory reserve volume of the lungs (ERV) and on the vital capacity were studied in the sitting and supine positions. These effects were compared to those produced by continuous negative-pressure breathing when the subjects were in air and were counteracted by positive pressure breathing during immersion. The depth of immersion was also related to definable anatomic landmarks. In the sitting position about one-fourth of the decrease in the ERV was accounted for by the hydrostatic pressure of the water on the abdomen and the remainder by the pressure on the thorax. Immersion to the level of the spinous process of the seventh cervical vertebra was equivalent to 28 cmH2o continuous negative pressure breathing in air. In the supine position, a comparable value was 8 cmH2o. These observations agree well with those of others if differences in the levels of immersion are accounted for."}
{"id": "PMID:1089115", "title": "Scanning electron microscopy of cavity margins finished with ultra-speed instruments.", "content": "The quality of the margins of the box portions of Class II cavities finished with rotating instruments at ultraspeed was assessed. In a closed row of teeth, an adequate finish was obtained at the embrasure margin where the burs rotated toward the tooth surface and into the cavity. The finish of the opposite embrasure margin and of the gingival margin was imperfect, regardless of the instrument used.", "contents": "Scanning electron microscopy of cavity margins finished with ultra-speed instruments. The quality of the margins of the box portions of Class II cavities finished with rotating instruments at ultraspeed was assessed. In a closed row of teeth, an adequate finish was obtained at the embrasure margin where the burs rotated toward the tooth surface and into the cavity. The finish of the opposite embrasure margin and of the gingival margin was imperfect, regardless of the instrument used."}
{"id": "PMID:1089114", "title": "Effect of temperature and humidity on the adhesive strength of orthodontic direct bonding materials.", "content": "The effects of temperature and humidity on the adhesive strength of two direct bonding orthodontic materials were investigated. Tensile testing showed that the adhesive bond was much stronger than the polycarbonate brackets. There is a significant increase in tensile and shearlike strength after 15-, 22-, and 30-day incubation at 37 C and 100% humidity, as compared with initial strength (after 30 minutes) at room temperature.", "contents": "Effect of temperature and humidity on the adhesive strength of orthodontic direct bonding materials. The effects of temperature and humidity on the adhesive strength of two direct bonding orthodontic materials were investigated. Tensile testing showed that the adhesive bond was much stronger than the polycarbonate brackets. There is a significant increase in tensile and shearlike strength after 15-, 22-, and 30-day incubation at 37 C and 100% humidity, as compared with initial strength (after 30 minutes) at room temperature."}
{"id": "PMID:1089125", "title": "Persistent individual differences in the bacterial flora of the skin of the forehead: numbers of propionibacteria.", "content": "The bacterial flora of the central area of the forehead of 48 adults was examined by a standardized swabbing procedure to determine the extent of individual variation. Propionibacteria were the most abundant organisms on most subjects. Their numbers per cm-2 of skin ranged from fewer than 6 to 1,100,000. Seventeen subjects had more than 100,000 and 16 subjects yielded fewer than 10,000. Six had fewer than 1,000. Differences with respect to sex and age were not statistically significant. Coagulase-negative cocci numbered from 25 to 75,000 per cm-2. There was no statistically significant correlation between numbers of propionibacteria and numbers of cocci. In order to distinguish trivial or transient individual differences from those of a more fundamental nature, 16 subjects were examined four times at intervals of several months with a total elapsed time of from 329 days to 523 dyas and a median of 385 days. Eight subjects were representative of the upper third and 8 the lower third of the population with respect to numbers of propionibacteria in the initial survey. Geometric mean values for propionibacteria per cm-2 of skin in the measurements of subjects with a rich population ranged from 152,686 to 280,867. Corresponding figures for the subjects with a sparse population were 1,215 to 3,639. Comparing geometric means by the t-test yielded p values of less than .001 in the second and fourth measurements and less than .01 in the third. It is concluded that a rich or a sparse population of propionibacteria is a stable individual characteristic of the skin of the forehead.", "contents": "Persistent individual differences in the bacterial flora of the skin of the forehead: numbers of propionibacteria. The bacterial flora of the central area of the forehead of 48 adults was examined by a standardized swabbing procedure to determine the extent of individual variation. Propionibacteria were the most abundant organisms on most subjects. Their numbers per cm-2 of skin ranged from fewer than 6 to 1,100,000. Seventeen subjects had more than 100,000 and 16 subjects yielded fewer than 10,000. Six had fewer than 1,000. Differences with respect to sex and age were not statistically significant. Coagulase-negative cocci numbered from 25 to 75,000 per cm-2. There was no statistically significant correlation between numbers of propionibacteria and numbers of cocci. In order to distinguish trivial or transient individual differences from those of a more fundamental nature, 16 subjects were examined four times at intervals of several months with a total elapsed time of from 329 days to 523 dyas and a median of 385 days. Eight subjects were representative of the upper third and 8 the lower third of the population with respect to numbers of propionibacteria in the initial survey. Geometric mean values for propionibacteria per cm-2 of skin in the measurements of subjects with a rich population ranged from 152,686 to 280,867. Corresponding figures for the subjects with a sparse population were 1,215 to 3,639. Comparing geometric means by the t-test yielded p values of less than .001 in the second and fourth measurements and less than .01 in the third. It is concluded that a rich or a sparse population of propionibacteria is a stable individual characteristic of the skin of the forehead."}
{"id": "PMID:1089116", "title": "Radioautographic evaluation of changes induced in the rat incisor by high-speed cavity preparation.", "content": "Rat incisors were evaluated radioautographically for metabolic changes induced by high-speed cavity preparation. Label uptake was reduced substantially in those odontoblasts whose processes had been cut; labeling also was diminished in all other areas of the pulp, especially in that area immediately adjacent to the cavity preparation.", "contents": "Radioautographic evaluation of changes induced in the rat incisor by high-speed cavity preparation. Rat incisors were evaluated radioautographically for metabolic changes induced by high-speed cavity preparation. Label uptake was reduced substantially in those odontoblasts whose processes had been cut; labeling also was diminished in all other areas of the pulp, especially in that area immediately adjacent to the cavity preparation."}
{"id": "PMID:1089126", "title": "Serum complement and immunity in experimental simian malaria. I. Cyclical alterations in C4 related to schizont rupture.", "content": "Previous studies indicated that the level of whole serum complement (C') falls during malaria, and that the fall in C' is linked to schizont rupture and the appearance of humoral antibody. For a more detailed study of the role of C' in malaria, levels of C4 were monitored in rhesus monkeys infected with Plasmodium coatneyi. After one to two weeks of infection, significant and abrupt declines in C4 levels occurred, and these were temporally related to the process of schizont rupture. The level of C4 began to fall within a few hours after the onset of schizont rupture and fell to values as low as 2% of prerupture levels. Over the next 24-36 hr, C4 returned to normal levels, and fell again during the next cycle of schizont rupture. In general, the decline in C4 levels was positively correlated with the onset of immune response (functional and serologic) and degree of parasitemia. This marked cyclincal depletion of C4 indicates tremendous turnover of C' in primates with malaria.", "contents": "Serum complement and immunity in experimental simian malaria. I. Cyclical alterations in C4 related to schizont rupture. Previous studies indicated that the level of whole serum complement (C') falls during malaria, and that the fall in C' is linked to schizont rupture and the appearance of humoral antibody. For a more detailed study of the role of C' in malaria, levels of C4 were monitored in rhesus monkeys infected with Plasmodium coatneyi. After one to two weeks of infection, significant and abrupt declines in C4 levels occurred, and these were temporally related to the process of schizont rupture. The level of C4 began to fall within a few hours after the onset of schizont rupture and fell to values as low as 2% of prerupture levels. Over the next 24-36 hr, C4 returned to normal levels, and fell again during the next cycle of schizont rupture. In general, the decline in C4 levels was positively correlated with the onset of immune response (functional and serologic) and degree of parasitemia. This marked cyclincal depletion of C4 indicates tremendous turnover of C' in primates with malaria."}
{"id": "PMID:1089127", "title": "Mouth lesions in iron-deficient anemia: relationship to Candida albicans in saliva and to impairment of lymphocyte transformation.", "content": "The atrophic glossitis and angular chilosis in patients with iron-deficient anemia are associated with infection of the mouth by Candida albicans. Saliva from these patients contained more Candida and supported the growth of Candida better than did saliva from a control group. The possibility that growth of Candida may be due to impaired lymphocyte function was investigated by measuring transformation of lymphocytes stimulated with phytohemagglutinin. Transformation was depressed in iron-deficient subjects and returned to normal after correction of their iron status. The lymphocyte count in the peripheral blood was also depressed and corrected by additional iron. Impaired lymphocyte function did not explain the mouth lesions and growth of Candida in saliva since lymphocyte transformation was equally depressed in patients with and without mouth lesions. A local factor, such as an effect of lack of iron on the resident vacterial flora of the mouth, may be important.", "contents": "Mouth lesions in iron-deficient anemia: relationship to Candida albicans in saliva and to impairment of lymphocyte transformation. The atrophic glossitis and angular chilosis in patients with iron-deficient anemia are associated with infection of the mouth by Candida albicans. Saliva from these patients contained more Candida and supported the growth of Candida better than did saliva from a control group. The possibility that growth of Candida may be due to impaired lymphocyte function was investigated by measuring transformation of lymphocytes stimulated with phytohemagglutinin. Transformation was depressed in iron-deficient subjects and returned to normal after correction of their iron status. The lymphocyte count in the peripheral blood was also depressed and corrected by additional iron. Impaired lymphocyte function did not explain the mouth lesions and growth of Candida in saliva since lymphocyte transformation was equally depressed in patients with and without mouth lesions. A local factor, such as an effect of lack of iron on the resident vacterial flora of the mouth, may be important."}
{"id": "PMID:1089128", "title": "The relation of K-antigen to virulence of Escherichia coli.", "content": "The content of K-antigen was determined for 100 strains of Escherichia coli isolated from the blood of patients with bacteremia. 30 strains isolated from urine cultures, and 30 strains isolated from the feces of patients recently admitted to the hospital. The K-antigen content of urinary isolates of E. coli was significantly greater than that of strains isolated from feces, as noted by other workers. In contrast, the amount of K-antigen of blood culture isolates was not significantly greater than that of fecal isolates and was significantly lower than that of urinary E. coli isolates. No correlation could be detected between the K-antigen content of blood culture isolates and the severity and outcome of the bacteremia.", "contents": "The relation of K-antigen to virulence of Escherichia coli. The content of K-antigen was determined for 100 strains of Escherichia coli isolated from the blood of patients with bacteremia. 30 strains isolated from urine cultures, and 30 strains isolated from the feces of patients recently admitted to the hospital. The K-antigen content of urinary isolates of E. coli was significantly greater than that of strains isolated from feces, as noted by other workers. In contrast, the amount of K-antigen of blood culture isolates was not significantly greater than that of fecal isolates and was significantly lower than that of urinary E. coli isolates. No correlation could be detected between the K-antigen content of blood culture isolates and the severity and outcome of the bacteremia."}
{"id": "PMID:1089129", "title": "Demonstration during life of rabies antigen in humans.", "content": "In three cases of human rabies, in which the diagnosis was proved postmortem, rabies antigen was detected by direct immunofluorescence of frozen sections of facial skin. The antigen was thought to be in nerve fibers in association with hair follicles. Development of this technique might enable the establishment of a method for the diagnosis of human rabies during life.", "contents": "Demonstration during life of rabies antigen in humans. In three cases of human rabies, in which the diagnosis was proved postmortem, rabies antigen was detected by direct immunofluorescence of frozen sections of facial skin. The antigen was thought to be in nerve fibers in association with hair follicles. Development of this technique might enable the establishment of a method for the diagnosis of human rabies during life."}
{"id": "PMID:1089131", "title": "Quantative analysis of the vectorcardiogram in obesity. The effects of weight reduction.", "content": "Vectorcardiograms (VCG) recorded in 37 subjects with marked chronic EXOGENOUS OBESITY (AVERAGE WEIGHT: 285 LBS.) WERE COMPARED before and after a significant weight reduction (average weight loss: 86 lbs). They were also compared with the VCGs of 293 age and sex matched controls with normal body weight. The SVEC III corrected orthogonal lead system was used, and out of several hundred vectorcardiographic measurements obtained by computer processing, 59 measurements representing various scalar, planar and spatial voltage and angular measurements were selected for study and comparisons. No significant differences were found between the measurements of obese subjects and those of the controls. Of 59 VCG measurements, 13 showed significant differences after weight reduction (paired t test) although they remained within the range of normal controls. There was a trend toward decrease of P and QRS amplitudes after weight reduction. There were no significant changes in the angular measurements. Although they are statistically significant these changes in voltage are too small to be detected in clinical vectorcardiography. The possible decrease of a preexisting myocardial hypertrophy superimposed onto the changes in the anatomy of the thorax might explain the effects of weight reduction. In general the reproducibility of VCG measurements obtained by the SVEC III system was greater than that reported in day-to-day operation with the Frank system in normal subjects.", "contents": "Quantative analysis of the vectorcardiogram in obesity. The effects of weight reduction. Vectorcardiograms (VCG) recorded in 37 subjects with marked chronic EXOGENOUS OBESITY (AVERAGE WEIGHT: 285 LBS.) WERE COMPARED before and after a significant weight reduction (average weight loss: 86 lbs). They were also compared with the VCGs of 293 age and sex matched controls with normal body weight. The SVEC III corrected orthogonal lead system was used, and out of several hundred vectorcardiographic measurements obtained by computer processing, 59 measurements representing various scalar, planar and spatial voltage and angular measurements were selected for study and comparisons. No significant differences were found between the measurements of obese subjects and those of the controls. Of 59 VCG measurements, 13 showed significant differences after weight reduction (paired t test) although they remained within the range of normal controls. There was a trend toward decrease of P and QRS amplitudes after weight reduction. There were no significant changes in the angular measurements. Although they are statistically significant these changes in voltage are too small to be detected in clinical vectorcardiography. The possible decrease of a preexisting myocardial hypertrophy superimposed onto the changes in the anatomy of the thorax might explain the effects of weight reduction. In general the reproducibility of VCG measurements obtained by the SVEC III system was greater than that reported in day-to-day operation with the Frank system in normal subjects."}
{"id": "PMID:1089133", "title": "Junctional reciprocating tachycardias. The permanent and paroxysmal forms of A-V nodal reciprocating tachycardias.", "content": "Stimulation technics have demonstrated a reciprocating mechanism in many supraventricular tachycardias previously classified from a purely morphologic point of view. Three conditions are required for the creation of a circus movement: a potential circuit pathway, undirectional block in this curcuit, and slowed conduction. While all three conditions are readily apparent in reciprocating tachycardias of the WPW syndrome, two or even all of these factors may be concealed in the others forms. Paroxysmal reciprocating tachycardias are characterized by prolongation of the P-R interval in the beat immediately preceding the tachycardia, and are generally accepted as being related to longitudinal dissociation of the A-V node, though the possibility of unidirectional (anterograde) block of an extra-nodal accessory pathway should be appreciated. Permanent reciprocating tachycardias start after a normal P-R interval when the sinus cycle reaches a critical value. Both paroxysmal and permanent forms of reciprocating tachycardia must be differentiated from tachycardias located in the atria: one of the most reliable features of reciprocating tachycardia is the existence of a 1:1 A-V relationship which cannot be altered without interrupting the tachycardia. Study of capture phenomena during the tachycardia, and the modes of termination not only permit the demonstration of the reentry mechanism but may also determine more precisely the actual location of the circus movement.", "contents": "Junctional reciprocating tachycardias. The permanent and paroxysmal forms of A-V nodal reciprocating tachycardias. Stimulation technics have demonstrated a reciprocating mechanism in many supraventricular tachycardias previously classified from a purely morphologic point of view. Three conditions are required for the creation of a circus movement: a potential circuit pathway, undirectional block in this curcuit, and slowed conduction. While all three conditions are readily apparent in reciprocating tachycardias of the WPW syndrome, two or even all of these factors may be concealed in the others forms. Paroxysmal reciprocating tachycardias are characterized by prolongation of the P-R interval in the beat immediately preceding the tachycardia, and are generally accepted as being related to longitudinal dissociation of the A-V node, though the possibility of unidirectional (anterograde) block of an extra-nodal accessory pathway should be appreciated. Permanent reciprocating tachycardias start after a normal P-R interval when the sinus cycle reaches a critical value. Both paroxysmal and permanent forms of reciprocating tachycardia must be differentiated from tachycardias located in the atria: one of the most reliable features of reciprocating tachycardia is the existence of a 1:1 A-V relationship which cannot be altered without interrupting the tachycardia. Study of capture phenomena during the tachycardia, and the modes of termination not only permit the demonstration of the reentry mechanism but may also determine more precisely the actual location of the circus movement."}
{"id": "PMID:1089136", "title": "Somatotopic representation of hindlimb skin in cat dorsal horn.", "content": "Single-unit exploration of the dorsal horn of segments L4-S2 of unanesthetized cats with the neuraxis transected at lower thoracic levels reveals a somototopic organization in the horizontal plane. The dorsal horn dermatomes correspond closely to the dermatomes of the corresponding dorsal roots, and the ML gradient is equally well described by two different projection schemes: a distoproximal gradient and a ventrodorsal one (5, 33). There is no evidence of segmental discontinuity of the map. As is the case in other nuclear regions of the CNS, the relative area devoted to projections from the foot is disproportionately large relative to the area devoted to skin regions of similar size which are located more proximally on the limb. From our data, and from the close correspondence to anatomical data obtained by others, we suggest that at least some cutaneous afferent fibers from a given skin area project directly to any dorsal horn region where that skin area is represented. This assumption, together with the organization of the dorsal horn map, yields a model which predicts a precise somatotopic organization of presynaptic neuropil in the substantia gelatinosa.", "contents": "Somatotopic representation of hindlimb skin in cat dorsal horn. Single-unit exploration of the dorsal horn of segments L4-S2 of unanesthetized cats with the neuraxis transected at lower thoracic levels reveals a somototopic organization in the horizontal plane. The dorsal horn dermatomes correspond closely to the dermatomes of the corresponding dorsal roots, and the ML gradient is equally well described by two different projection schemes: a distoproximal gradient and a ventrodorsal one (5, 33). There is no evidence of segmental discontinuity of the map. As is the case in other nuclear regions of the CNS, the relative area devoted to projections from the foot is disproportionately large relative to the area devoted to skin regions of similar size which are located more proximally on the limb. From our data, and from the close correspondence to anatomical data obtained by others, we suggest that at least some cutaneous afferent fibers from a given skin area project directly to any dorsal horn region where that skin area is represented. This assumption, together with the organization of the dorsal horn map, yields a model which predicts a precise somatotopic organization of presynaptic neuropil in the substantia gelatinosa."}
{"id": "PMID:1089137", "title": "Follow-up comparison of hydrocephalus with and without myelomeningocele.", "content": "A series of 454 hydrocephalic patients with and without myelomeningocele and with and without treatment is reviewed. The survival rates for hydrocephalus alone and for hydrocephalus with myelodysplasia are comparable. The authors reach the conclusion that treatment of the hycrocephalic process and its complications is the most critical therapeutic consideration. Mental retardation is the major unalterable cause for failure to develop independence; some lesser emotional causes can be modified by encouragement. Repeated reassessment of the patient's condition and adjustment are important. Before treatment is started parents or guardians should be fully informed of the child's future potential for independent life and mental development.", "contents": "Follow-up comparison of hydrocephalus with and without myelomeningocele. A series of 454 hydrocephalic patients with and without myelomeningocele and with and without treatment is reviewed. The survival rates for hydrocephalus alone and for hydrocephalus with myelodysplasia are comparable. The authors reach the conclusion that treatment of the hycrocephalic process and its complications is the most critical therapeutic consideration. Mental retardation is the major unalterable cause for failure to develop independence; some lesser emotional causes can be modified by encouragement. Repeated reassessment of the patient's condition and adjustment are important. Before treatment is started parents or guardians should be fully informed of the child's future potential for independent life and mental development."}
{"id": "PMID:1089139", "title": "A comparison of 111In with 52Fe and 99mTc-sulfur colloid for bone marrow scanning.", "content": "Under most circumstances 52Fe, 111In, and colloid show a similar distribution of marrow. The lesser uptake of 111In by liver and spleen may occasionally be of value in permitting visualization of that portion of the spinal marrow obscured by these organs in the colloid scan. However, in red cell aplasia, when there is dissociation between phagocytic and erythropoietic functions, scanning with 111In gives no information about erythropoietic tissue distribution. Therefore, indium cannot be used as an analog for iron in the study of the hematopoietic system.", "contents": "A comparison of 111In with 52Fe and 99mTc-sulfur colloid for bone marrow scanning. Under most circumstances 52Fe, 111In, and colloid show a similar distribution of marrow. The lesser uptake of 111In by liver and spleen may occasionally be of value in permitting visualization of that portion of the spinal marrow obscured by these organs in the colloid scan. However, in red cell aplasia, when there is dissociation between phagocytic and erythropoietic functions, scanning with 111In gives no information about erythropoietic tissue distribution. Therefore, indium cannot be used as an analog for iron in the study of the hematopoietic system."}
{"id": "PMID:1089140", "title": "The medical ethics of bone marrow transplantation in childhood.", "content": "Bone marrow transplantation has been utilized for the treatment of severe aplastic anemia in a program at The Children's Hospital Medical Center. A protocol has been developed to help ensure that the interests and rightful claims of all involved parties will be taken into account. Three cases are presented. In one case all involved parties concurred with the plan for bone marrow transplantation. In a second case the prospective donor was deemed mentally incompetent to give truly informed consent for the procedure. In a third case the parents were unwilling to have their child undergo transplantation. The ethical issues raised in these cases suggest the need for further development of a moral technology to enhance decision-making competence and to aid in the recognition and application of the rights of children without impeding the advancement of pediatric medicine.", "contents": "The medical ethics of bone marrow transplantation in childhood. Bone marrow transplantation has been utilized for the treatment of severe aplastic anemia in a program at The Children's Hospital Medical Center. A protocol has been developed to help ensure that the interests and rightful claims of all involved parties will be taken into account. Three cases are presented. In one case all involved parties concurred with the plan for bone marrow transplantation. In a second case the prospective donor was deemed mentally incompetent to give truly informed consent for the procedure. In a third case the parents were unwilling to have their child undergo transplantation. The ethical issues raised in these cases suggest the need for further development of a moral technology to enhance decision-making competence and to aid in the recognition and application of the rights of children without impeding the advancement of pediatric medicine."}
{"id": "PMID:1089142", "title": "Mesangiocapillary glomerulonephritis: changes in glomerular morphology with long-term alternate-day prednisone therapy.", "content": "In all of eight patients with mesangiocapillary glomerulonephritis who received prednisone in an alternate-day schedule for 3 to 9 hr, the glomerule showed a marked reduction in mesangial cellularity and complete regression of mesangial interposition. Subendothelial deposits disappeared but intramembranous dense deposits, when present previously, persisted. Repair was incomplete in that a few mesangial deposits were still present, immunoflourescence was unchanged, and several of the patients developed focal mesangial scarring and increased numbers of global bodies. Functional glomerule and glomerular tuft segments, however, showed greatly improved morphology. There was no evidence of deterioration of renal function over a follow-up period of 5 to 11.5 yr.", "contents": "Mesangiocapillary glomerulonephritis: changes in glomerular morphology with long-term alternate-day prednisone therapy. In all of eight patients with mesangiocapillary glomerulonephritis who received prednisone in an alternate-day schedule for 3 to 9 hr, the glomerule showed a marked reduction in mesangial cellularity and complete regression of mesangial interposition. Subendothelial deposits disappeared but intramembranous dense deposits, when present previously, persisted. Repair was incomplete in that a few mesangial deposits were still present, immunoflourescence was unchanged, and several of the patients developed focal mesangial scarring and increased numbers of global bodies. Functional glomerule and glomerular tuft segments, however, showed greatly improved morphology. There was no evidence of deterioration of renal function over a follow-up period of 5 to 11.5 yr."}
{"id": "PMID:1089144", "title": "Clinical observations following root amputation in maxillary molar teeth.", "content": "Thirty-four maxillary molar teeth were evaluated from 11 to 84 months after root amputation. The most consistent finding was that although 24 were neither splinted nor supported in any way, only three developed mobility. Two of these were used as abutments for partial dentures. One other tooth was extracted due to recurrent lateral abscesses and subsequent involvement of the mesial furca.", "contents": "Clinical observations following root amputation in maxillary molar teeth. Thirty-four maxillary molar teeth were evaluated from 11 to 84 months after root amputation. The most consistent finding was that although 24 were neither splinted nor supported in any way, only three developed mobility. Two of these were used as abutments for partial dentures. One other tooth was extracted due to recurrent lateral abscesses and subsequent involvement of the mesial furca."}
{"id": "PMID:1089145", "title": "Development of dental plaque on epoxy resin crowns in man. A light and electron microscopic study.", "content": "A method was presented to fabricate epoxy resin crowns to be worn by human subjects requiring full crown restorations. These crowns were utilized in six young adults to study the internal structure of plaque after plaque formation periods of 1 and 3 days, 1 and 3 weeks and 2 months. This study confirmed previous findings that early plaque contains primarily coccal forms, with a shift to predominantly filamentous forms by 3 weeks. Early plaque growth seems to occur by the formation of columnar microcolonies which coalesce and grow by cell division within the colony in a direction perpendicular to the crown surface. Filamentous microorganisms appear in large numbers by 1 week. They appear to colonize the surface of the predominantly coccal plaque, eventually growing into it and replacing the coccal forms. The subgingival, mature plaque contains many motile forms including bacteria with unusual cell wall ultrastructures. Certain bacteria combine into distinctive bacterial aggregations resembling \"corn cobs\" and \"test tube brushes,\" the latter occurring exclusively in subgingival plaque. Spirochetes appear to grow preferentially on the external surface of subgingival plaque in close contact to the gingival tissue of the deepened sulcus. Their high concentration in the external layer of subgingival plaque suggests that because of their strategic location they may play an important role in the etiology of periodontal disease. Studies of well preserved plaque, possibly combined with the use of serological markers, can serve a useful role in identifying certain microorganisms in dental plaque. Because of their numbers and/or location in relation to periodontal tissues, some of these bacteria may warrant further studies as potential etiologic agents of certain forms of periodontal disease.", "contents": "Development of dental plaque on epoxy resin crowns in man. A light and electron microscopic study. A method was presented to fabricate epoxy resin crowns to be worn by human subjects requiring full crown restorations. These crowns were utilized in six young adults to study the internal structure of plaque after plaque formation periods of 1 and 3 days, 1 and 3 weeks and 2 months. This study confirmed previous findings that early plaque contains primarily coccal forms, with a shift to predominantly filamentous forms by 3 weeks. Early plaque growth seems to occur by the formation of columnar microcolonies which coalesce and grow by cell division within the colony in a direction perpendicular to the crown surface. Filamentous microorganisms appear in large numbers by 1 week. They appear to colonize the surface of the predominantly coccal plaque, eventually growing into it and replacing the coccal forms. The subgingival, mature plaque contains many motile forms including bacteria with unusual cell wall ultrastructures. Certain bacteria combine into distinctive bacterial aggregations resembling \"corn cobs\" and \"test tube brushes,\" the latter occurring exclusively in subgingival plaque. Spirochetes appear to grow preferentially on the external surface of subgingival plaque in close contact to the gingival tissue of the deepened sulcus. Their high concentration in the external layer of subgingival plaque suggests that because of their strategic location they may play an important role in the etiology of periodontal disease. Studies of well preserved plaque, possibly combined with the use of serological markers, can serve a useful role in identifying certain microorganisms in dental plaque. Because of their numbers and/or location in relation to periodontal tissues, some of these bacteria may warrant further studies as potential etiologic agents of certain forms of periodontal disease."}
{"id": "PMID:1089153", "title": "Mating, ovulation and corpus luteum function in the vole, Microtus agrestis.", "content": "A study of the relationship between the mating vehaviour of the vole and the induction of ovulation and CL function is described. A single intromission or an injection of LH-RF constitute stimuli which induce ovulation, but normally give rise to CL that degenerate soon after formation. More prolonged mating, or mechanical stimulation of the vagina and cervix given after a separate ovulatory stimulus, result in the maintenance of the CL. Mechanical genital stimulation is effective in inducing CL MAINTENANCE WHEN GIVEN UP TO 48 HR AFTER AN LH-RF injection. Similarities, therefore, are apparent between the vole, an induced oculator, and spontaneous ovulator, and spontaneous ovulators such as the rat, mouse and hamster.", "contents": "Mating, ovulation and corpus luteum function in the vole, Microtus agrestis. A study of the relationship between the mating vehaviour of the vole and the induction of ovulation and CL function is described. A single intromission or an injection of LH-RF constitute stimuli which induce ovulation, but normally give rise to CL that degenerate soon after formation. More prolonged mating, or mechanical stimulation of the vagina and cervix given after a separate ovulatory stimulus, result in the maintenance of the CL. Mechanical genital stimulation is effective in inducing CL MAINTENANCE WHEN GIVEN UP TO 48 HR AFTER AN LH-RF injection. Similarities, therefore, are apparent between the vole, an induced oculator, and spontaneous ovulator, and spontaneous ovulators such as the rat, mouse and hamster."}
{"id": "PMID:1089154", "title": "Scanning electron microscopy of the luminal epithelium of the mouse uterus.", "content": "Four methods of manipulating mouse uterine tissue during fixation, preparatory to examination of the luminal epithelium by scanning electron microscopy (S.E.M.), are described and the results assessed. The appearance of the epithelium varies according to the technique used, the choice of method depending on the type of information required from the S.E.M. study. Surface topography is preserved in a condition most closely resembling that of fresh tissue by opening the uterus and fixing the tissue flat. Critical examination of cellular and subcellular surface detail, however, depends on adequate spreading of the epithelial layer during fixation by distension of the uterine lumen under positive pressure. The method of tissue manipulation has a more profound effect on the appearance of the sample than any of the dehydration techniques which follow. For routine S.E.M. examinations at medium magnifications (smaller than X10,000), specimens which were air dried from alcohol, acetone or ether, or freeze dried, gave satisfactory results. Air drying from amyl acetate, and CO2 critical-point drying gave superior results at higher magnifications (greater than X10,000) with better preservation of individual microvilli.", "contents": "Scanning electron microscopy of the luminal epithelium of the mouse uterus. Four methods of manipulating mouse uterine tissue during fixation, preparatory to examination of the luminal epithelium by scanning electron microscopy (S.E.M.), are described and the results assessed. The appearance of the epithelium varies according to the technique used, the choice of method depending on the type of information required from the S.E.M. study. Surface topography is preserved in a condition most closely resembling that of fresh tissue by opening the uterus and fixing the tissue flat. Critical examination of cellular and subcellular surface detail, however, depends on adequate spreading of the epithelial layer during fixation by distension of the uterine lumen under positive pressure. The method of tissue manipulation has a more profound effect on the appearance of the sample than any of the dehydration techniques which follow. For routine S.E.M. examinations at medium magnifications (smaller than X10,000), specimens which were air dried from alcohol, acetone or ether, or freeze dried, gave satisfactory results. Air drying from amyl acetate, and CO2 critical-point drying gave superior results at higher magnifications (greater than X10,000) with better preservation of individual microvilli."}
{"id": "PMID:1089155", "title": "Neuroendocrine mechanisms responsible for gonadotropin release.", "content": "Regulation of the ovulatory cycle involves the interdependence of the adenohypophysis, the ovaries, the CNS, and the external environment. Several distinctions in brain functions and the overlapping control of several neuro-endocrine systems are pointed out. Important neuro-anatomical relationships between basomedial hypothalamus and other brain regions are noted. The anatomical structure is correlated with the control of gonadotropin secretion. Reflexive ovulation is considered as a special instance of external environmental influence. Data suggests that both lower mammals and human primates may show reflexive as well as spontaneous ovulation.", "contents": "Neuroendocrine mechanisms responsible for gonadotropin release. Regulation of the ovulatory cycle involves the interdependence of the adenohypophysis, the ovaries, the CNS, and the external environment. Several distinctions in brain functions and the overlapping control of several neuro-endocrine systems are pointed out. Important neuro-anatomical relationships between basomedial hypothalamus and other brain regions are noted. The anatomical structure is correlated with the control of gonadotropin secretion. Reflexive ovulation is considered as a special instance of external environmental influence. Data suggests that both lower mammals and human primates may show reflexive as well as spontaneous ovulation."}
{"id": "PMID:1089156", "title": "Cesarean section in the massively obese.", "content": "A series of 24 abdominal sections were performed on patients weighing 114 Kg or over. A detailed analysis of the indications, vital statistics, and medical, surgical, and post operative complications were made. There were no serious complications. The authors conclude that reluctance to perform abdominal section because of obesity is not warranted.", "contents": "Cesarean section in the massively obese. A series of 24 abdominal sections were performed on patients weighing 114 Kg or over. A detailed analysis of the indications, vital statistics, and medical, surgical, and post operative complications were made. There were no serious complications. The authors conclude that reluctance to perform abdominal section because of obesity is not warranted."}
{"id": "PMID:1089157", "title": "The immature HPO axis.", "content": "One cause or anovulation may be an immature hypothalamic-pituitary-ovarian axis. The fact that initial menstrual cycles are usually irregular and often anovulatory implies that a maturation process is taking place in the HPO axis and that cyclic ovulatory menstruation begins only when adequate maturation occurs. Moreover, the external appearance of the ovary of a severely oligomenorrheic or amenorrheic female frequently is similar to that of a prepubertal female--this is, the ovary appears normal in size of slightly smaller, has a smooth, glistening surface without convolutions, and its capsule-like outer surface reveals few, if any, underlying follicles. A reasonable assumption is that there is inadequate gonadotropin stimulation of these ovaries possibly as a result of an immature HPO axis. The studies by radioimmunoassay of FSH and LH levels in prepubertal and pubertal  females offer no statistical data by which to measure the maturity of the HPO axis, although consistently low FSH and LH levels may prove meaningful. Studies of FSH and LH in patients exhibiting gonadal dysgenesis neither support or disprove the immature HPO axis theory, but studies of idiopathic sexual precocity tend to support it. Studies using LH-RF in prepubertal and pubertal females indicate a pattern of response which may give useful information in the area.", "contents": "The immature HPO axis. One cause or anovulation may be an immature hypothalamic-pituitary-ovarian axis. The fact that initial menstrual cycles are usually irregular and often anovulatory implies that a maturation process is taking place in the HPO axis and that cyclic ovulatory menstruation begins only when adequate maturation occurs. Moreover, the external appearance of the ovary of a severely oligomenorrheic or amenorrheic female frequently is similar to that of a prepubertal female--this is, the ovary appears normal in size of slightly smaller, has a smooth, glistening surface without convolutions, and its capsule-like outer surface reveals few, if any, underlying follicles. A reasonable assumption is that there is inadequate gonadotropin stimulation of these ovaries possibly as a result of an immature HPO axis. The studies by radioimmunoassay of FSH and LH levels in prepubertal and pubertal  females offer no statistical data by which to measure the maturity of the HPO axis, although consistently low FSH and LH levels may prove meaningful. Studies of FSH and LH in patients exhibiting gonadal dysgenesis neither support or disprove the immature HPO axis theory, but studies of idiopathic sexual precocity tend to support it. Studies using LH-RF in prepubertal and pubertal females indicate a pattern of response which may give useful information in the area."}
{"id": "PMID:1089158", "title": "Field trials of a gonococcal vaccine.", "content": "Several small, well controlled field trials with a gonococcus vaccine have been initiated. The results to date indicate that the vaccine elicits only mild reactions in humans, and that it will produce antibodies of possible significance in over 90 per cent of vaccines. Its value in preventing gonorrhea has yet to be established. Studies towards this end are now being considered.", "contents": "Field trials of a gonococcal vaccine. Several small, well controlled field trials with a gonococcus vaccine have been initiated. The results to date indicate that the vaccine elicits only mild reactions in humans, and that it will produce antibodies of possible significance in over 90 per cent of vaccines. Its value in preventing gonorrhea has yet to be established. Studies towards this end are now being considered."}
{"id": "PMID:1089160", "title": "Release of sediment-bound fecal coliforms by dredging.", "content": "Fecal coliform concentrations increased significantly (F test) in the immediate vicinity of a maintenance dredging operation in the Mississippi River navigation channel. Increased counts were attributed to the disturbance and relocation of bottom sediments by dredging and a concomitant release of sediment-bound fecal coliforms.", "contents": "Release of sediment-bound fecal coliforms by dredging. Fecal coliform concentrations increased significantly (F test) in the immediate vicinity of a maintenance dredging operation in the Mississippi River navigation channel. Increased counts were attributed to the disturbance and relocation of bottom sediments by dredging and a concomitant release of sediment-bound fecal coliforms."}
{"id": "PMID:1089161", "title": "Medium-scale production of citrinin by Penicillium citrinum in a semisynthetic medium.", "content": "A convenient method is described for the production of up to 1.75 g of citrinin per liter by Penicillium citrinum growing in stationary culture in a 5-gallon (18.925 liters) carboy containing 4 liters of 4% sucrose and 2% yeast extract medium.", "contents": "Medium-scale production of citrinin by Penicillium citrinum in a semisynthetic medium. A convenient method is described for the production of up to 1.75 g of citrinin per liter by Penicillium citrinum growing in stationary culture in a 5-gallon (18.925 liters) carboy containing 4 liters of 4% sucrose and 2% yeast extract medium."}
{"id": "PMID:1089162", "title": "Evaluation of a medium for the rapid recovery of Escherichia coli from shellfish.", "content": "A medium (A-1) which shortens the time necessary to identify and enumerate Escherichia coli found in estuarine water was evaluated for use for recovery of E. coli found in shellfish. Productivity of E. coli by this medium was comparable to that of the lengthier American Public Health Association method, and the occurrence of false positives was substantially reduced.", "contents": "Evaluation of a medium for the rapid recovery of Escherichia coli from shellfish. A medium (A-1) which shortens the time necessary to identify and enumerate Escherichia coli found in estuarine water was evaluated for use for recovery of E. coli found in shellfish. Productivity of E. coli by this medium was comparable to that of the lengthier American Public Health Association method, and the occurrence of false positives was substantially reduced."}
{"id": "PMID:1089163", "title": "Irradiation of Escherichia coli in the visible spectrum with a tunable organic-dye laser energy source.", "content": "Pulsed laser energy was shown to be effective in inhibiting the growth of Escherichia coli. The irradiation source was derived from a tunable organic-dye laser utilizing rhodamine 6G (590 plus or minus 5 nm) solutions as lasing media. The organisms, suspended in nutrient broth, were irradiated both with and without an exogenous photosensitizer. One photosensitizer (toluidine blue) did not appreciably alter the inhibitory effect observed. In the presence of acridine orange, however, some additional growth occurred.", "contents": "Irradiation of Escherichia coli in the visible spectrum with a tunable organic-dye laser energy source. Pulsed laser energy was shown to be effective in inhibiting the growth of Escherichia coli. The irradiation source was derived from a tunable organic-dye laser utilizing rhodamine 6G (590 plus or minus 5 nm) solutions as lasing media. The organisms, suspended in nutrient broth, were irradiated both with and without an exogenous photosensitizer. One photosensitizer (toluidine blue) did not appreciably alter the inhibitory effect observed. In the presence of acridine orange, however, some additional growth occurred."}
{"id": "PMID:1089167", "title": "Evaluation of a muscle relaxant: dantrolene sodium (Dantrium).", "content": "Dantrolene sodium (Dantrium) is a skeletal muscle relaxant, unique in that it acts on the muscle itself. It should be considered for use in patients with skeletal muscle spasticity who are in a stable neurological state. After careful adjustment of the dose, a substantial number of such patients will experience one or more of the following benefits: (1) a reduction in pain, (2) an increased ability to make use of residual motor function, (3) a reduction in the level of nursing care required, (4) an increased ability to utilize devices, and (5) an increased ability to participate in rehabilitation. The drug should not be used when reduced spasticity will decrease functional ability. The adverse effects generally are transient; some are the result of central nervous system depression.", "contents": "Evaluation of a muscle relaxant: dantrolene sodium (Dantrium). Dantrolene sodium (Dantrium) is a skeletal muscle relaxant, unique in that it acts on the muscle itself. It should be considered for use in patients with skeletal muscle spasticity who are in a stable neurological state. After careful adjustment of the dose, a substantial number of such patients will experience one or more of the following benefits: (1) a reduction in pain, (2) an increased ability to make use of residual motor function, (3) a reduction in the level of nursing care required, (4) an increased ability to utilize devices, and (5) an increased ability to participate in rehabilitation. The drug should not be used when reduced spasticity will decrease functional ability. The adverse effects generally are transient; some are the result of central nervous system depression."}
{"id": "PMID:1089173", "title": "Complexities of smoking education.", "content": "Cigarette smoking is a many-headed Hydra, which grows new smokers' heads as fast as they are lopped off. Efforts to change smokers' behavior usually reach a  point of diminishing returns, as evidenced by experiences in smoking withdrawal clinics and in public anti-smoking education campaigns. Obviously, many smokers are refactory to anti-smoking education, a situation which has generated a great deal of pharmacological, neurochemical, psychological, and sociological research. The National Clearinghouse for Smoking and Health in the U.S.A. has used a \"gradualist\" or permissive approach in anti-smoking messages, aimed at less hazardous smoking \"if you must smoke,\" rather than at complete cessation, and found this approach to be useful. U.S.A. school-children are exposed to anti-smoking education in lower grades than formerly, when it was offered in high school. Thus a population of as yet non-smoking children is being educated. Perhaps the adult non-smokers should be enlisted in an effort to create a social climate wherein smoking is not an acceptable behavior-an approach reflecting the concept of smoking as a social disease. Health education addressed to the receptive non-smoker would circumvent the refractory smoker. A theme with appeal to non-smokers is the protest against environmental pollution by smokers. It is possible that if a movement by non-smokers against smoking would gather momentum, it could turn the present accepting social climate into one rejecting smoking, thus striking at the regeneration of the Hydra: the incessant recruitment of smokers among ex-smokers and non-smokers. With recruitment below replacement level, the Hydra would in time wither away.", "contents": "Complexities of smoking education. Cigarette smoking is a many-headed Hydra, which grows new smokers' heads as fast as they are lopped off. Efforts to change smokers' behavior usually reach a  point of diminishing returns, as evidenced by experiences in smoking withdrawal clinics and in public anti-smoking education campaigns. Obviously, many smokers are refactory to anti-smoking education, a situation which has generated a great deal of pharmacological, neurochemical, psychological, and sociological research. The National Clearinghouse for Smoking and Health in the U.S.A. has used a \"gradualist\" or permissive approach in anti-smoking messages, aimed at less hazardous smoking \"if you must smoke,\" rather than at complete cessation, and found this approach to be useful. U.S.A. school-children are exposed to anti-smoking education in lower grades than formerly, when it was offered in high school. Thus a population of as yet non-smoking children is being educated. Perhaps the adult non-smokers should be enlisted in an effort to create a social climate wherein smoking is not an acceptable behavior-an approach reflecting the concept of smoking as a social disease. Health education addressed to the receptive non-smoker would circumvent the refractory smoker. A theme with appeal to non-smokers is the protest against environmental pollution by smokers. It is possible that if a movement by non-smokers against smoking would gather momentum, it could turn the present accepting social climate into one rejecting smoking, thus striking at the regeneration of the Hydra: the incessant recruitment of smokers among ex-smokers and non-smokers. With recruitment below replacement level, the Hydra would in time wither away."}
{"id": "PMID:1089174", "title": "Continuous sutures in replacement of mitral and tricuspid valves with prostheses.", "content": "Four years' experience with 363 patients, in whom 360 mitral and 65 tricuspid prosthetic valves have been implanted with single continuous sutures, is presented. The occurrence of prosthetic valve detachment is particularly discussed. There were 4 cases of detachment of the prosthetic mitral valve and no cases of tricupsid dehiscence. All 4 patients with detached valves required reoperation. Calcifications that penetrate into the annulus and myxomatous degeneration of the valve are considered more important factors in the production of prosthetic cases so long as a uniform distribution of stitches is maintained (not too close to each other) and excessive tension in the suture line is avoided.", "contents": "Continuous sutures in replacement of mitral and tricuspid valves with prostheses. Four years' experience with 363 patients, in whom 360 mitral and 65 tricuspid prosthetic valves have been implanted with single continuous sutures, is presented. The occurrence of prosthetic valve detachment is particularly discussed. There were 4 cases of detachment of the prosthetic mitral valve and no cases of tricupsid dehiscence. All 4 patients with detached valves required reoperation. Calcifications that penetrate into the annulus and myxomatous degeneration of the valve are considered more important factors in the production of prosthetic cases so long as a uniform distribution of stitches is maintained (not too close to each other) and excessive tension in the suture line is avoided."}
{"id": "PMID:1089175", "title": "Surgical considerations in patients undergoing repeat median sternotomy.", "content": "A retrospective review of 122 repeat median sternotomy incisions in 100 consecutive patients was made to evaluate complications and management. Eighty-one patients had one repeat sternotomy, )6 had two repeat sternotomies, and 3 had three repeat sternotomies. All had valve procedures in the past and were reoperated upon for progressive rheumatic valvular disease or for complications related to the prostheses. Complications included operative hemorrhage in 8 patients, postoperative hemorrhage in 2, seroma in 4, and dehiscence, wound infection, and hematoma in 1 patient each. The most serious complication was hemorrhage and was the cause of the only operative death. Seven other patients survived hemorrhage encountered during repeat sternotomy. Control of massive hemorrhage during repeat sternotomy has been possible due to an organized approach to the patient with adhesions from previous surgery.", "contents": "Surgical considerations in patients undergoing repeat median sternotomy. A retrospective review of 122 repeat median sternotomy incisions in 100 consecutive patients was made to evaluate complications and management. Eighty-one patients had one repeat sternotomy, )6 had two repeat sternotomies, and 3 had three repeat sternotomies. All had valve procedures in the past and were reoperated upon for progressive rheumatic valvular disease or for complications related to the prostheses. Complications included operative hemorrhage in 8 patients, postoperative hemorrhage in 2, seroma in 4, and dehiscence, wound infection, and hematoma in 1 patient each. The most serious complication was hemorrhage and was the cause of the only operative death. Seven other patients survived hemorrhage encountered during repeat sternotomy. Control of massive hemorrhage during repeat sternotomy has been possible due to an organized approach to the patient with adhesions from previous surgery."}
{"id": "PMID:1089176", "title": "Selective sputum cultures. A bronchial culture technique using a modified nasotracheal suction catheter with a sterile, inner telescoping cannula.", "content": "The contamination of expectorated or catheter-aspirated sputum specimens by pathogenic microorganisms which have colonized the nose and oropharynx remains a formidable obstacle to the accurate interpretation of sputum cultures. This problem is encountered in all forms of acute and chronic bronchopulmonary infection. A standard suctioning technique via a nasotracheal catheter has been modified with a telescoping sterile inner cannula to obtain uncontaminated bronchopulmonary secretions for culture. Bacteriologic results of selective bronchial cultures obtained in 18 patients following major chest surgery have provided important considerations concerning the prophylactic use of antibiotics. The telescoping cannula method is a simple, safe, and practical means of selectively monitoring the bacteriologic flora of the lower respiratory tract.", "contents": "Selective sputum cultures. A bronchial culture technique using a modified nasotracheal suction catheter with a sterile, inner telescoping cannula. The contamination of expectorated or catheter-aspirated sputum specimens by pathogenic microorganisms which have colonized the nose and oropharynx remains a formidable obstacle to the accurate interpretation of sputum cultures. This problem is encountered in all forms of acute and chronic bronchopulmonary infection. A standard suctioning technique via a nasotracheal catheter has been modified with a telescoping sterile inner cannula to obtain uncontaminated bronchopulmonary secretions for culture. Bacteriologic results of selective bronchial cultures obtained in 18 patients following major chest surgery have provided important considerations concerning the prophylactic use of antibiotics. The telescoping cannula method is a simple, safe, and practical means of selectively monitoring the bacteriologic flora of the lower respiratory tract."}
{"id": "PMID:1089182", "title": "Sociobehavioral determinants of compliance with health and medical care recommendations.", "content": "Over the past two decades, hundreds of articles, editorials, and commentaries have been published describing the considerable disruptive effects on quality of care of individuals noncompliance with health and medical advice. While much research has been directed at determining factors responsible for poor compliance, past studies have tended to focus upon easily measured characteristics of the patients, regimen, or illness which, unfortunately, are usually neither predictive nor alterable. This paper systematically reviews the literature on patient acceptance of recommended health behaviors, attempting to find social-psychological and related variables which have proven to be consistent predictors of compliance. The review suggests that certain health beliefs (especailly personal estimates of vulnerability to, and seriousness of, the disease, and faith in the efficacy of care), health-related motivations, perceptions of psychological and other costs of the recommended action, various aspects of the doctor-patient relationship, and social influence are the most productive dimensions for present intervention and further exploration. Building upon an earlier formulation, an hypothesized model is presented which combines these elements for explaining and predicting compliance behavior. Further research should, with standardized questionnaires and analysis techniques, employ prospective, experimental designs for a variety of population groups, settings, and regimens, to evaluate the ability of practical attempts to modify the model variables and thus enhance compliance.", "contents": "Sociobehavioral determinants of compliance with health and medical care recommendations. Over the past two decades, hundreds of articles, editorials, and commentaries have been published describing the considerable disruptive effects on quality of care of individuals noncompliance with health and medical advice. While much research has been directed at determining factors responsible for poor compliance, past studies have tended to focus upon easily measured characteristics of the patients, regimen, or illness which, unfortunately, are usually neither predictive nor alterable. This paper systematically reviews the literature on patient acceptance of recommended health behaviors, attempting to find social-psychological and related variables which have proven to be consistent predictors of compliance. The review suggests that certain health beliefs (especailly personal estimates of vulnerability to, and seriousness of, the disease, and faith in the efficacy of care), health-related motivations, perceptions of psychological and other costs of the recommended action, various aspects of the doctor-patient relationship, and social influence are the most productive dimensions for present intervention and further exploration. Building upon an earlier formulation, an hypothesized model is presented which combines these elements for explaining and predicting compliance behavior. Further research should, with standardized questionnaires and analysis techniques, employ prospective, experimental designs for a variety of population groups, settings, and regimens, to evaluate the ability of practical attempts to modify the model variables and thus enhance compliance."}
{"id": "PMID:1089189", "title": "Metabolic factors in the control of energy stores.", "content": "We have examined some of the factors involved in the control of food intake and integrated the data using methods of systems analysis from biomedical engineering. The central hypothesis is that energy stored in the body is a regulated variable. Alterations in the quantity of stored calories initiates changes designed to restore the store of calories to its original level. These responses are both short-term and long-term in nature. They involve integrating data on the quantities of protein, carbohydrate, and lipid stores in the body, probably through such feedback signals as amino acids, glucose, glycerol, and free fatty acids.", "contents": "Metabolic factors in the control of energy stores. We have examined some of the factors involved in the control of food intake and integrated the data using methods of systems analysis from biomedical engineering. The central hypothesis is that energy stored in the body is a regulated variable. Alterations in the quantity of stored calories initiates changes designed to restore the store of calories to its original level. These responses are both short-term and long-term in nature. They involve integrating data on the quantities of protein, carbohydrate, and lipid stores in the body, probably through such feedback signals as amino acids, glucose, glycerol, and free fatty acids."}
{"id": "PMID:1089191", "title": "Effects of corticosteroid therapy on human monocyte function.", "content": "Since high-dose corticosteroid therapy appears to impair cellular defense mechanisms, this study examined its effect on human monocyte function. Fifteen normal volunteers were studied before and after a three-day course of prednisone therapy (50 mg every 12 hours for six doses). A transient period of monocytopenia occurred during the first few hours of therapy. Monocyte killing of Staphylococcus aureus was reduced in nine subjects from 5.6 plus or minus 0.2 (plus or minus S.E.) X 10-6 organisms before to 1.3 plus or minus 0.4 x 10-6 organisms at completion of therapy (p less than 0.01). Similary, killing of Candida tropicalis four subjects fell from 9.3 plus or minus 0.6 to 0.6 plus or minus 0.3 x 10-6 organisma (p less than 0.01). Bactericidal activity returned to normal levels 48 hours after the last dose of prednisone. These same monocyte preparations had normal or increased chemotactic response, phagocytic rate of cryptococci, hexosemonophosphate-shunt response to phagocytosis and ultrastructural characteristics. This impairment of bactericidal and fungicidal activity during prednisone therapy may contribute to the infectious complications seen in patients receiving comparable doses of corticosteroids.", "contents": "Effects of corticosteroid therapy on human monocyte function. Since high-dose corticosteroid therapy appears to impair cellular defense mechanisms, this study examined its effect on human monocyte function. Fifteen normal volunteers were studied before and after a three-day course of prednisone therapy (50 mg every 12 hours for six doses). A transient period of monocytopenia occurred during the first few hours of therapy. Monocyte killing of Staphylococcus aureus was reduced in nine subjects from 5.6 plus or minus 0.2 (plus or minus S.E.) X 10-6 organisms before to 1.3 plus or minus 0.4 x 10-6 organisms at completion of therapy (p less than 0.01). Similary, killing of Candida tropicalis four subjects fell from 9.3 plus or minus 0.6 to 0.6 plus or minus 0.3 x 10-6 organisma (p less than 0.01). Bactericidal activity returned to normal levels 48 hours after the last dose of prednisone. These same monocyte preparations had normal or increased chemotactic response, phagocytic rate of cryptococci, hexosemonophosphate-shunt response to phagocytosis and ultrastructural characteristics. This impairment of bactericidal and fungicidal activity during prednisone therapy may contribute to the infectious complications seen in patients receiving comparable doses of corticosteroids."}
{"id": "PMID:1089192", "title": "Restoration of normal pituitary gonadotropin reserve by administration of luteinizing-hormone-releasing hormone in patients with hypogonadotropic hypogonadism.", "content": "To elucidate whether diminished pituitary gonadotropin reserve can be restored by repeated stimulation with luteinizing-hormone-releasing hormone, plasma luteinizing hormone and follicly-stimulating hormone responses were studied before and after daily intravenous infusion of 400 mug of luteinizing-hormone-releasing hormone for two to 23 days, in patients with hypogonadotropic hypogonadism of various causes. In five of nine patients with isolated gonadotropin deficiency, the impaired plasma luteinizing hormone response was restored to normal after treatment for seven days or more, whereas it was unchanged in four patients treated for less than five days. However, six patients with anorexia nervosa regained normal responses after three to five days' treatment. Five of nine patients with organic hypothalamopituitary lesions also showed normal responsiveness after five to seven days' treatment. These results suggest that the response to the test after repeated administration of luteinizing-hormone-releasing hormone is of value for the diagnosis of hypogonadism of hypothalamic origin.", "contents": "Restoration of normal pituitary gonadotropin reserve by administration of luteinizing-hormone-releasing hormone in patients with hypogonadotropic hypogonadism. To elucidate whether diminished pituitary gonadotropin reserve can be restored by repeated stimulation with luteinizing-hormone-releasing hormone, plasma luteinizing hormone and follicly-stimulating hormone responses were studied before and after daily intravenous infusion of 400 mug of luteinizing-hormone-releasing hormone for two to 23 days, in patients with hypogonadotropic hypogonadism of various causes. In five of nine patients with isolated gonadotropin deficiency, the impaired plasma luteinizing hormone response was restored to normal after treatment for seven days or more, whereas it was unchanged in four patients treated for less than five days. However, six patients with anorexia nervosa regained normal responses after three to five days' treatment. Five of nine patients with organic hypothalamopituitary lesions also showed normal responsiveness after five to seven days' treatment. These results suggest that the response to the test after repeated administration of luteinizing-hormone-releasing hormone is of value for the diagnosis of hypogonadism of hypothalamic origin."}
{"id": "PMID:1089193", "title": "Psychiatry: the battered child of medicine.", "content": "Psychiatry has had a troubled history. After the French Revolution \"moral treatment\" brought to America a period of effective and humane hospital treatment, but this progress was corrupted by the Industrial Revolution, and psychiatry was rejected by society as well as by medicine. Although the Freudian enlightenment and the introduction of social psychiatry have led to greater acceptance, today's criticisms are strident. Psychiatry is criticized for imprecise diagnosis, conceptual vagaries, jargon, therapeutic impotence and class bias. The American system of mental-health care is seen by many as a disaster. The federal comprehensive Community Mental Health Act has been aborted at an early stage. Skills involved in the practice of psychotherapy are not unique to the profession. Social ills, over which psychiatry has little control, play a large part in causing mental disability and retardation. Nevertheless, though embattled, psychiatry has contributed to medical practice and to the humane consciousness of society.", "contents": "Psychiatry: the battered child of medicine. Psychiatry has had a troubled history. After the French Revolution \"moral treatment\" brought to America a period of effective and humane hospital treatment, but this progress was corrupted by the Industrial Revolution, and psychiatry was rejected by society as well as by medicine. Although the Freudian enlightenment and the introduction of social psychiatry have led to greater acceptance, today's criticisms are strident. Psychiatry is criticized for imprecise diagnosis, conceptual vagaries, jargon, therapeutic impotence and class bias. The American system of mental-health care is seen by many as a disaster. The federal comprehensive Community Mental Health Act has been aborted at an early stage. Skills involved in the practice of psychotherapy are not unique to the profession. Social ills, over which psychiatry has little control, play a large part in causing mental disability and retardation. Nevertheless, though embattled, psychiatry has contributed to medical practice and to the humane consciousness of society."}
{"id": "PMID:1089210", "title": "The nucleotide sequence of an RNA polymerase binding site on bacteriophage fd DNA.", "content": "The nucleotide sequence of the RNA polymerase binding site at a promotor on fd RF DNA has been determined in comparison with the starting sequence of RNA initiated at this promoter. The RNA polymerase binding site contained the startpoint of transcription in the centre and a region with twofold symmetry in the non-transcribed part.", "contents": "The nucleotide sequence of an RNA polymerase binding site on bacteriophage fd DNA. The nucleotide sequence of the RNA polymerase binding site at a promotor on fd RF DNA has been determined in comparison with the starting sequence of RNA initiated at this promoter. The RNA polymerase binding site contained the startpoint of transcription in the centre and a region with twofold symmetry in the non-transcribed part."}
{"id": "PMID:1089225", "title": "Prospective bacteriologic study of women undergoing hysterectomy.", "content": "A prospective bacteriologic study on 67 women undergoing either vaginal or abdominal hysterectomy was done to elucidate causes of debrile morbidity. Forty-one percent of the vaginal and 35% of the abdominal hysterectomy patients met the criteria for febrile morbidity postoperatively. Preoperative and 24-hour postoperative urine samples were obtained by transvaginal bladder tap, and the freshly cut edge of the vaginal cuff was routinely swabbed following removal of the uterus. In addition, the subcutaneous layer was cultured during closure of the abdominal wound. Escherichia coli, Enterococcus, and alpha- and beta-Streptococcus were the predominant organisms recovered. The importance of routine vaginal cuff cultures at the time of surgery for the treatment of postoperative morbidity is discussed.", "contents": "Prospective bacteriologic study of women undergoing hysterectomy. A prospective bacteriologic study on 67 women undergoing either vaginal or abdominal hysterectomy was done to elucidate causes of debrile morbidity. Forty-one percent of the vaginal and 35% of the abdominal hysterectomy patients met the criteria for febrile morbidity postoperatively. Preoperative and 24-hour postoperative urine samples were obtained by transvaginal bladder tap, and the freshly cut edge of the vaginal cuff was routinely swabbed following removal of the uterus. In addition, the subcutaneous layer was cultured during closure of the abdominal wound. Escherichia coli, Enterococcus, and alpha- and beta-Streptococcus were the predominant organisms recovered. The importance of routine vaginal cuff cultures at the time of surgery for the treatment of postoperative morbidity is discussed."}
{"id": "PMID:1089237", "title": "Pharmaceutic factors affecting pediatric compliance.", "content": "Evaluation of treatment given at home was studied in children with otitis media who were seen in an outpatient clinic. Full compliance was present in only 5% of the initial 100 patients (Study A). Practical factors limiting their compliance included inadequate dispensing of medication at drug stores, 15%; incorrect therapy schedule, 36%; early termination, 37%; spilled medicine, 7%; therapy shared, 5%. Because of these findings, a plan was implemented (Study B) in which hospital pharmacy personnel gave patient families verbal and written instructions for administering medications that were dispensed, together with a calibrated measuring device and a calendar to record doses taken. Full compliance was raised to 51% in this pilot group (of 33 patients) as compared with 8.5% in 20 concurrent controls who went to neighborhood drug stores. The importance of detailed therapy instructions is stressed. The potential role of the pharmacist in improving compliance is demonstrated.", "contents": "Pharmaceutic factors affecting pediatric compliance. Evaluation of treatment given at home was studied in children with otitis media who were seen in an outpatient clinic. Full compliance was present in only 5% of the initial 100 patients (Study A). Practical factors limiting their compliance included inadequate dispensing of medication at drug stores, 15%; incorrect therapy schedule, 36%; early termination, 37%; spilled medicine, 7%; therapy shared, 5%. Because of these findings, a plan was implemented (Study B) in which hospital pharmacy personnel gave patient families verbal and written instructions for administering medications that were dispensed, together with a calibrated measuring device and a calendar to record doses taken. Full compliance was raised to 51% in this pilot group (of 33 patients) as compared with 8.5% in 20 concurrent controls who went to neighborhood drug stores. The importance of detailed therapy instructions is stressed. The potential role of the pharmacist in improving compliance is demonstrated."}
{"id": "PMID:1089238", "title": "The Japanese operation for biliary atresia: remedy or mischief?", "content": "A case illustration is given of an infant with biliary atresia who had the Kasai operation more than 1 1/2 years ago and is now, by all biochemical and histological criteria, cured. The early results with this operation in 14 patients are similar to those reported by the Japanese, that is, 25% have had sustained postoperative bile drainage. Success has never been achieved in infants older than 4 months. A long-held notion in this country to withhold operation in infants with jaundice until after 4 months has thus far militated against a critical and impartial evaluation of the Kasai operation. A plea is made to complete diagnostic work-up for jaundice during the first months of life so that the operation, if elected, may have a genuine chance for legitimate long-term analysis.", "contents": "The Japanese operation for biliary atresia: remedy or mischief? A case illustration is given of an infant with biliary atresia who had the Kasai operation more than 1 1/2 years ago and is now, by all biochemical and histological criteria, cured. The early results with this operation in 14 patients are similar to those reported by the Japanese, that is, 25% have had sustained postoperative bile drainage. Success has never been achieved in infants older than 4 months. A long-held notion in this country to withhold operation in infants with jaundice until after 4 months has thus far militated against a critical and impartial evaluation of the Kasai operation. A plea is made to complete diagnostic work-up for jaundice during the first months of life so that the operation, if elected, may have a genuine chance for legitimate long-term analysis."}
{"id": "PMID:1089240", "title": "A review of five years' experience with rubella vaccine in the United States.", "content": "National morbidity figures show a decline in reported rubella and congenital rubella syndrome since 1969, concurrent with widespread use of rubella vaccine. In addition, no nationwide outbreak, such as the 1963-1964 epidemic, has occurred, though on the basis of long-term secular trends, one would be expected between 1970 and 1974. Recent rubella outbreaks have occurred in unimmunized students in high schools and universities, and there appears to have been a slight upward shift in the age-specific incidence of rubella in the United States since the beginning of widespread immunization. Currently available vaccines have provided durable protection to date, and, although reinfection is known to occur following vaccination, it has not proven a risk to the pregnant woman. There is a small but significant incidence of adverse reactions and a potential risk to the woman who is vaccinated during pregnancy. These data indicate that rubella vaccines are safe and effective. They also imply that rubella vaccines, as they are currently applied, have been successful in reducing the morbidity of congenital rubella syndrome, although continued surveillance will be necessary to confirm this trend.", "contents": "A review of five years' experience with rubella vaccine in the United States. National morbidity figures show a decline in reported rubella and congenital rubella syndrome since 1969, concurrent with widespread use of rubella vaccine. In addition, no nationwide outbreak, such as the 1963-1964 epidemic, has occurred, though on the basis of long-term secular trends, one would be expected between 1970 and 1974. Recent rubella outbreaks have occurred in unimmunized students in high schools and universities, and there appears to have been a slight upward shift in the age-specific incidence of rubella in the United States since the beginning of widespread immunization. Currently available vaccines have provided durable protection to date, and, although reinfection is known to occur following vaccination, it has not proven a risk to the pregnant woman. There is a small but significant incidence of adverse reactions and a potential risk to the woman who is vaccinated during pregnancy. These data indicate that rubella vaccines are safe and effective. They also imply that rubella vaccines, as they are currently applied, have been successful in reducing the morbidity of congenital rubella syndrome, although continued surveillance will be necessary to confirm this trend."}
{"id": "PMID:1089241", "title": "Oxygen plus pressure plus time: the etiology of bronchopulmonary dysplasia.", "content": "Ten infants developed the chronic pulmonary syndrome designated bronchopulmonary dysplasia, following artificial (assisted) ventilation using a volume-regulated positive-pressure respirator. Despite previous reports implicating elevated oxygen concentrations for prolonged periods of time, only two infants required more than 80% oxygen for more than 24 hours, and only one infant required 60% oxygen for more than 100 hours. \"Immature\" lungs when exposed to inspired oxygen concentrations over 40% for as little as three days via positive-pressure ventilation may develop bronchopulmonary dysplasia. The role of the underlying disease and the relationship with Wilson-Mikity syndrome remain uncertain.", "contents": "Oxygen plus pressure plus time: the etiology of bronchopulmonary dysplasia. Ten infants developed the chronic pulmonary syndrome designated bronchopulmonary dysplasia, following artificial (assisted) ventilation using a volume-regulated positive-pressure respirator. Despite previous reports implicating elevated oxygen concentrations for prolonged periods of time, only two infants required more than 80% oxygen for more than 24 hours, and only one infant required 60% oxygen for more than 100 hours. \"Immature\" lungs when exposed to inspired oxygen concentrations over 40% for as little as three days via positive-pressure ventilation may develop bronchopulmonary dysplasia. The role of the underlying disease and the relationship with Wilson-Mikity syndrome remain uncertain."}
{"id": "PMID:1089242", "title": "Bronchopulmonary dysplasia and lung rupture in hyaline membrane disease: influence of continuous distending pressure.", "content": "Review of 158 patients with hyaline membrane disease was undertaken. The introduction of artificial ventilation with a positive end-expiratory pressure (IPPB and PEEP) has doubled the prevalence of pneumothorax, pneumomediastinum and interstitial emphysema from 20.7% to 39.7%). Continuous negative distending pressure during spontaneous ventilation (CNP) was associated with a prevalence of lung rupture similar to that occurring spontaneously (4.8%). No patient treated with CNP alone developed bronchopulmonary dysplasia. Patients treated with IPPB with PEEP had a marked decreased prevalence of bronchopulmonary dysplasia (17.2%) when compared to patients treated with IPPB alone (36.2%), probably related to the enhanced overdistension of relatively normal areas of the lung may be related to the increased prevalence of lung rupture seen during IPPB with PEEP.", "contents": "Bronchopulmonary dysplasia and lung rupture in hyaline membrane disease: influence of continuous distending pressure. Review of 158 patients with hyaline membrane disease was undertaken. The introduction of artificial ventilation with a positive end-expiratory pressure (IPPB and PEEP) has doubled the prevalence of pneumothorax, pneumomediastinum and interstitial emphysema from 20.7% to 39.7%). Continuous negative distending pressure during spontaneous ventilation (CNP) was associated with a prevalence of lung rupture similar to that occurring spontaneously (4.8%). No patient treated with CNP alone developed bronchopulmonary dysplasia. Patients treated with IPPB with PEEP had a marked decreased prevalence of bronchopulmonary dysplasia (17.2%) when compared to patients treated with IPPB alone (36.2%), probably related to the enhanced overdistension of relatively normal areas of the lung may be related to the increased prevalence of lung rupture seen during IPPB with PEEP."}
{"id": "PMID:1089252", "title": "The circulating plasma volume of the foetal lamb as an index of its weight and rate of weight gain (g/day) in the last third of gestation.", "content": "In a series of foetal lambs weighing between 1,100 and 5,228 g, the circulating plasma volume was estimated by the dye dilution method, using Evans Blue, to test the possibility that the plasma volume could be used as an index of foetal weight in chronic studies. The data, analysed by the method of least squares regression, indicate that plasma volume and foetal weight are closely correlated (R-2 equals 0.922) and linearly so in the range of data studied. There was no evidence that the relation differed for singlets and twins. A single equation, Y equals 71.8 plus 10.11 X--where Y is the estimated weight and X the plasma volume, can be used to predict the weight from plasma volume in both. Some results of the application of the method in chronic studies are presented.", "contents": "The circulating plasma volume of the foetal lamb as an index of its weight and rate of weight gain (g/day) in the last third of gestation. In a series of foetal lambs weighing between 1,100 and 5,228 g, the circulating plasma volume was estimated by the dye dilution method, using Evans Blue, to test the possibility that the plasma volume could be used as an index of foetal weight in chronic studies. The data, analysed by the method of least squares regression, indicate that plasma volume and foetal weight are closely correlated (R-2 equals 0.922) and linearly so in the range of data studied. There was no evidence that the relation differed for singlets and twins. A single equation, Y equals 71.8 plus 10.11 X--where Y is the estimated weight and X the plasma volume, can be used to predict the weight from plasma volume in both. Some results of the application of the method in chronic studies are presented."}
{"id": "PMID:1089259", "title": "Renal localization of gallium-67 citrate.", "content": "Renal localization of 67Ga citrate has been observed in 1.7% of 2,000 whole-body gallium scans in three major diseases: lymphoreticular neoplasms, leukemia, and malignant melanoma. Correlation of this finding with autopsy data in 13 cases revealed 9 with tumor and 2 with an inflammatory process. Findings in two cases were normal. In the remaining patients, correlation with abnormal urograms and laboratory data was poor. Nevertheless, gallium localization in the kidneys may be the first indicator of renal involvement with either tumor or inflammation.", "contents": "Renal localization of gallium-67 citrate. Renal localization of 67Ga citrate has been observed in 1.7% of 2,000 whole-body gallium scans in three major diseases: lymphoreticular neoplasms, leukemia, and malignant melanoma. Correlation of this finding with autopsy data in 13 cases revealed 9 with tumor and 2 with an inflammatory process. Findings in two cases were normal. In the remaining patients, correlation with abnormal urograms and laboratory data was poor. Nevertheless, gallium localization in the kidneys may be the first indicator of renal involvement with either tumor or inflammation."}
{"id": "PMID:1089308", "title": "Changes of the coliform populations of the shoreline waters, Shediac, New Brunswick.", "content": "This paper presents the results of a survey of the changes in the coliform populations of the shoreline waters, Shediac, New Brunswick. The sanitary quality of these waters showed more than 1000 organisms/100 ml of coliforms thereby indicating post-winter and pre-summer contamination of these waters. The membrane filter method is found to be equally efficient to that of the MPN method. The sanitary quality of these waters in relation to other ecological parameters like temperatures, pH salinity and availability of oxygen are considered.", "contents": "Changes of the coliform populations of the shoreline waters, Shediac, New Brunswick. This paper presents the results of a survey of the changes in the coliform populations of the shoreline waters, Shediac, New Brunswick. The sanitary quality of these waters showed more than 1000 organisms/100 ml of coliforms thereby indicating post-winter and pre-summer contamination of these waters. The membrane filter method is found to be equally efficient to that of the MPN method. The sanitary quality of these waters in relation to other ecological parameters like temperatures, pH salinity and availability of oxygen are considered."}
{"id": "PMID:1089309", "title": "Vesicular binesis: glucose effect on insulin secretory vesicles.", "content": "Binesis is a process whereby the membrane of the insuline secretory vesicle in the beta cell forms a lingula that indents the vesicular membrane of an adjoining secretory vesicle of the plasma membrane. Vesicular binesis in beta cells increases when islets of Langerhans are incubated at a stimulatory glucose concentration (300 miligrams per 100 milliliters). These vesicular membrane alterations may be the morphological concomitants of activation of the insulin secretory vesicle, and indicate an active role for the vesicle and its membrane in the release mechanism.", "contents": "Vesicular binesis: glucose effect on insulin secretory vesicles. Binesis is a process whereby the membrane of the insuline secretory vesicle in the beta cell forms a lingula that indents the vesicular membrane of an adjoining secretory vesicle of the plasma membrane. Vesicular binesis in beta cells increases when islets of Langerhans are incubated at a stimulatory glucose concentration (300 miligrams per 100 milliliters). These vesicular membrane alterations may be the morphological concomitants of activation of the insulin secretory vesicle, and indicate an active role for the vesicle and its membrane in the release mechanism."}
{"id": "PMID:1089310", "title": "F factor promotes turnover of stable RNA in escherichia coli.", "content": "Male bacteria that contain and srnA- mutant allele degrade their \"stable\" RNA massively after RNA synthesis is blocked at 42 degrees C; a normal F- female strain shows no such RNA breakdown unless both the srnA- allele and maleness (F factor) are introduced.", "contents": "F factor promotes turnover of stable RNA in escherichia coli. Male bacteria that contain and srnA- mutant allele degrade their \"stable\" RNA massively after RNA synthesis is blocked at 42 degrees C; a normal F- female strain shows no such RNA breakdown unless both the srnA- allele and maleness (F factor) are introduced."}
{"id": "PMID:1089322", "title": "Papilledema: its recognition and relation to increased intracranial pressure.", "content": "This has been a review on the subject of papilledema from a clinical, pathologic and experimental point of view. Terminology has been clarified. The term papilledema should be reserved for those patients with optic disc edema caused by increased intracranial pressure. The forms of papilledema which are identifiable are: 1. Early (incipient) form which develops into an acute type and then into a fully developed form; 2. Fully developed form, characterized by obscured disc margins, hemorrhages and ischemic infarcts; 3. Chronic papilledema which may persist (the name \"vintage\" papilledema may be applied to those patients affected in varying degrees up to several years); and 4. Chronic atrophic papilledema which, as the name implies, illustrates a form we do not see as frequently as in years past. It is the type we are now trying to prevent and in this effort we are fortunately achieveing some success. The importance of the intracranial expanding lesions and the influence of the rapidity of elevation and duration of intracranial hypertension on the optic nerve has been considered, based on the clinical and experimental work to date. The pathogenesis of papilledema has been considered in some detail. A unified whole is difficult to arrive at in such a complex situation. Recent advances in our understanding of anatomic, mechanical (sheath space pressure), tissue and vascular pressure as related to increased intracranial pressure have been described. A hydrostatic mechanism brings these factors together in a reasonable, although admittedly not completely proven concept of a mechanism for the development of papilledema. Clarification of terminology and clinical appearance of the various forms of disc edema related to intracranial pressure are of practical value in the diagnosis and management of each patient we see with this clinical entity.", "contents": "Papilledema: its recognition and relation to increased intracranial pressure. This has been a review on the subject of papilledema from a clinical, pathologic and experimental point of view. Terminology has been clarified. The term papilledema should be reserved for those patients with optic disc edema caused by increased intracranial pressure. The forms of papilledema which are identifiable are: 1. Early (incipient) form which develops into an acute type and then into a fully developed form; 2. Fully developed form, characterized by obscured disc margins, hemorrhages and ischemic infarcts; 3. Chronic papilledema which may persist (the name \"vintage\" papilledema may be applied to those patients affected in varying degrees up to several years); and 4. Chronic atrophic papilledema which, as the name implies, illustrates a form we do not see as frequently as in years past. It is the type we are now trying to prevent and in this effort we are fortunately achieveing some success. The importance of the intracranial expanding lesions and the influence of the rapidity of elevation and duration of intracranial hypertension on the optic nerve has been considered, based on the clinical and experimental work to date. The pathogenesis of papilledema has been considered in some detail. A unified whole is difficult to arrive at in such a complex situation. Recent advances in our understanding of anatomic, mechanical (sheath space pressure), tissue and vascular pressure as related to increased intracranial pressure have been described. A hydrostatic mechanism brings these factors together in a reasonable, although admittedly not completely proven concept of a mechanism for the development of papilledema. Clarification of terminology and clinical appearance of the various forms of disc edema related to intracranial pressure are of practical value in the diagnosis and management of each patient we see with this clinical entity."}
{"id": "PMID:1089323", "title": "Intraocular reticulum-cell sarcoma: clinico-pathologic study of four cases and review of the literature.", "content": "Four patients with primary intraocular reticulum-cell sarcoma were presented. All of the patients were followed at the Wilmer Institute for periods of up to ten years. All had decreased visual acuity and a uveitis that was refractory to standard forms of therapy. In one case, the correct diagnosis was made clinically. At autopsy, two of the patients had systemic reticulum-cell sarcoma, a third had intracranial reticulum-cell sarcoma, and in a fourth case the disease process was confined to the eye. The literature is reviewed and the clinico-pathologic features of the total of 14 cases are analyzed. It is emphasized that a recalcitrant uveitis was present in almost all of the cases reported. Differential diagnosis and methods of diagnosis are discussed.", "contents": "Intraocular reticulum-cell sarcoma: clinico-pathologic study of four cases and review of the literature. Four patients with primary intraocular reticulum-cell sarcoma were presented. All of the patients were followed at the Wilmer Institute for periods of up to ten years. All had decreased visual acuity and a uveitis that was refractory to standard forms of therapy. In one case, the correct diagnosis was made clinically. At autopsy, two of the patients had systemic reticulum-cell sarcoma, a third had intracranial reticulum-cell sarcoma, and in a fourth case the disease process was confined to the eye. The literature is reviewed and the clinico-pathologic features of the total of 14 cases are analyzed. It is emphasized that a recalcitrant uveitis was present in almost all of the cases reported. Differential diagnosis and methods of diagnosis are discussed."}
{"id": "PMID:1089338", "title": "[Usability of methods for the determination of minute cardiac volume under clinical conditions].", "content": "Methods for determination of cardiac output are described and their evaluation and possibilities to use them in clinical practice mentioned. Out of the invasive methods apart from the classical Fick's method, the dye dilution method is of advantage during catheterization, being relatively simple and ready to be easily repeated if necessary directly at the bed side. The common disadvantage of all invasive methods is the disturbance of the patient's basal state. Noninvasive methods yield results suitable more or less for orientation only. From these techniques the indirect method of Fick using the rebreathing of CO2 is simple and can be applied at the bed side. So called physical methods are already obsolete at present.", "contents": "[Usability of methods for the determination of minute cardiac volume under clinical conditions]. Methods for determination of cardiac output are described and their evaluation and possibilities to use them in clinical practice mentioned. Out of the invasive methods apart from the classical Fick's method, the dye dilution method is of advantage during catheterization, being relatively simple and ready to be easily repeated if necessary directly at the bed side. The common disadvantage of all invasive methods is the disturbance of the patient's basal state. Noninvasive methods yield results suitable more or less for orientation only. From these techniques the indirect method of Fick using the rebreathing of CO2 is simple and can be applied at the bed side. So called physical methods are already obsolete at present."}
{"id": "PMID:1089352", "title": "Bone changes in young mice with impaired lymphoid system.", "content": "Disturbances of osseal growth were observed in young mice with their lymphoid system affected by antihymocyte serum or mitolactol (dibromodulcitol) treatment. These bone changes were similar to those observed in germ-free and neonatally thymectomized mice as well as in mice suffering from a graft vs. host reaction. Their severity was in direct correlation with the disturbance of the thymus dependent lymphoid system. Not only immunological adaptation but also normal bone growth appears to require an intact thymus and thymus dependent lymphoid system.", "contents": "Bone changes in young mice with impaired lymphoid system. Disturbances of osseal growth were observed in young mice with their lymphoid system affected by antihymocyte serum or mitolactol (dibromodulcitol) treatment. These bone changes were similar to those observed in germ-free and neonatally thymectomized mice as well as in mice suffering from a graft vs. host reaction. Their severity was in direct correlation with the disturbance of the thymus dependent lymphoid system. Not only immunological adaptation but also normal bone growth appears to require an intact thymus and thymus dependent lymphoid system."}
{"id": "PMID:1089353", "title": "Chromosome aberrations in cells infected with various strains of measles virus.", "content": "The incidence of chromosome fragmentation and/or breakage has been studied in cultured human embryonic diploid cells infected with different wild and vaccinal strains of measles virus. It seemed that for wild strains the mean incidence of fragmentation was two times higher, that of breakage two times lower than for the vaccinal strains.", "contents": "Chromosome aberrations in cells infected with various strains of measles virus. The incidence of chromosome fragmentation and/or breakage has been studied in cultured human embryonic diploid cells infected with different wild and vaccinal strains of measles virus. It seemed that for wild strains the mean incidence of fragmentation was two times higher, that of breakage two times lower than for the vaccinal strains."}
{"id": "PMID:1089354", "title": "A clinical isolate of Salmonella typhi requiring anaerobic conditions for primary isolation.", "content": "An unusual isolate of Salmonella typhi from a patient with typhoid fever is described. The organism grew only on anaerobic subculture from multiple blood cultures. After repeated transfer the organism adapted to aerobic growth but still required carbon dioxide.", "contents": "A clinical isolate of Salmonella typhi requiring anaerobic conditions for primary isolation. An unusual isolate of Salmonella typhi from a patient with typhoid fever is described. The organism grew only on anaerobic subculture from multiple blood cultures. After repeated transfer the organism adapted to aerobic growth but still required carbon dioxide."}
{"id": "PMID:1089355", "title": "Cardiobacterium homonis endocarditis. Characterization of the unusual organisms and review of the literature.", "content": "A case of subacute bacterial endocarditis in which Cardiobacterium hominis was isolated from the blood of a 55-year old woman who had rheumatic heart disease is reported. A survey of the literature revealed very few reports in which this organism has been implicated in human lesions. The colonies grew after 48 hours of incubation in a candle jar. They were small, convex, nonhemolytic, and oxidase-positive. The indole reaction was positive, the catalase and nitrate reactions were negative, and acid reaction was obtained from the following carbohydrates: glucose, maltose, mannitol, sucrose, and sorbitol. The morphologic and biochemical properties served to distinguish these organisms from similar bacteria implicated in human disease, such as Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, Streptobacillus moniliformis, and HB-1.", "contents": "Cardiobacterium homonis endocarditis. Characterization of the unusual organisms and review of the literature. A case of subacute bacterial endocarditis in which Cardiobacterium hominis was isolated from the blood of a 55-year old woman who had rheumatic heart disease is reported. A survey of the literature revealed very few reports in which this organism has been implicated in human lesions. The colonies grew after 48 hours of incubation in a candle jar. They were small, convex, nonhemolytic, and oxidase-positive. The indole reaction was positive, the catalase and nitrate reactions were negative, and acid reaction was obtained from the following carbohydrates: glucose, maltose, mannitol, sucrose, and sorbitol. The morphologic and biochemical properties served to distinguish these organisms from similar bacteria implicated in human disease, such as Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, Streptobacillus moniliformis, and HB-1."}
{"id": "PMID:1089356", "title": "Comparison of isotonic and radiometric-hypertonic cultures for the recovery of organisms from cerebrospinal, pleural and synovial fluids.", "content": "Two hundred eighteen (218) specimens of body fluids were examined by both isotonic and hypertonic culture methods. The specimens examined included 108 pleural fluids, 43 synovial fluids, and 67 cerebrospinal fluids. Organisms were recovered in 99 instances by means of the isotonic system and in 140 instances by the hypertonic system. The hypertonic system also involved the use of radiometric assay for the detection of positive cultures. In all 140 cases, detection was accomplished within 36 hours after collection of the specimen by the radiometric method, while only 26 specimens showed any visual evidence of bacterial growth. Of the 218 patients involved, 198 were receiving antimicrobial therapy in which the antimicrobial agent attacked the bacterial cell wall.", "contents": "Comparison of isotonic and radiometric-hypertonic cultures for the recovery of organisms from cerebrospinal, pleural and synovial fluids. Two hundred eighteen (218) specimens of body fluids were examined by both isotonic and hypertonic culture methods. The specimens examined included 108 pleural fluids, 43 synovial fluids, and 67 cerebrospinal fluids. Organisms were recovered in 99 instances by means of the isotonic system and in 140 instances by the hypertonic system. The hypertonic system also involved the use of radiometric assay for the detection of positive cultures. In all 140 cases, detection was accomplished within 36 hours after collection of the specimen by the radiometric method, while only 26 specimens showed any visual evidence of bacterial growth. Of the 218 patients involved, 198 were receiving antimicrobial therapy in which the antimicrobial agent attacked the bacterial cell wall."}
{"id": "PMID:1089357", "title": "A simple method of inflation--fixation and air drying of lungs.", "content": "The various technics for pulmonary inflation are briefly discussed. A method of pulmonary inflation, fixation and drying is described. This preparation provides lung specimens suitable for many modes of study or demonstration, as well as excellent histologic detail. The technic is simple enough to use routinely in the study of pulmonary disease outside of a research center.", "contents": "A simple method of inflation--fixation and air drying of lungs. The various technics for pulmonary inflation are briefly discussed. A method of pulmonary inflation, fixation and drying is described. This preparation provides lung specimens suitable for many modes of study or demonstration, as well as excellent histologic detail. The technic is simple enough to use routinely in the study of pulmonary disease outside of a research center."}
{"id": "PMID:1089358", "title": "Identity conflicts in adoptees.", "content": "A review of the literature, and interviews with a large number of adoptees who have experienced reunions with their birth parents, indicates that adoptees are more vulnerable than the population at large to the development of identity problems in late adolescence and young adulthood.", "contents": "Identity conflicts in adoptees. A review of the literature, and interviews with a large number of adoptees who have experienced reunions with their birth parents, indicates that adoptees are more vulnerable than the population at large to the development of identity problems in late adolescence and young adulthood."}
{"id": "PMID:1089359", "title": "Hyperactivity in preschoolers and the effect of methylphenidate.", "content": "Observations of nursery behavior and tests of cognitive style and motor impulsivity were carried out with 28 hyperactive preschool children of normal intelligence and 26 matched control children. The hyperactive group was also observed and tested on methylphenidate and placebo. Methylphenidate was found to reduce hyperactivity at home, but it did not improve nursery behavior or psychological functioning. Unwanted side effects made the drug less useful at this age than in older hyperactive children.", "contents": "Hyperactivity in preschoolers and the effect of methylphenidate. Observations of nursery behavior and tests of cognitive style and motor impulsivity were carried out with 28 hyperactive preschool children of normal intelligence and 26 matched control children. The hyperactive group was also observed and tested on methylphenidate and placebo. Methylphenidate was found to reduce hyperactivity at home, but it did not improve nursery behavior or psychological functioning. Unwanted side effects made the drug less useful at this age than in older hyperactive children."}
{"id": "PMID:1089360", "title": "Peer behavior conceptualized as a variable influencing infant and toddler development.", "content": "It is hypothesized that interaction between infants may serve to facilitate their overall behavioral development, particularly if the environment is structured to maximize the developmental quality of the interaction. The developmental literature, which has largely ignorned the potential value of infant peer interaction is reviewed, and several lines of psychological theory and investigation are cited in support of the hypothesis. Development of a technology for enhancing infant interaction is proposed, and initial research questions are suggested.", "contents": "Peer behavior conceptualized as a variable influencing infant and toddler development. It is hypothesized that interaction between infants may serve to facilitate their overall behavioral development, particularly if the environment is structured to maximize the developmental quality of the interaction. The developmental literature, which has largely ignorned the potential value of infant peer interaction is reviewed, and several lines of psychological theory and investigation are cited in support of the hypothesis. Development of a technology for enhancing infant interaction is proposed, and initial research questions are suggested."}
{"id": "PMID:1089362", "title": "Psychiatric training and practice in the People's Republic of China.", "content": "Improved political relations between the United States and the People's Republic of China have led to closer contact in many areas. The authors trace the development of psychiatric training and practice in China with emphasis on the years after 1949. Since then, China has expanded psychiatric services to meet the needs of a vast population and has developed a theoretical approach to psychiatry that emphasizes sociopolitical factors. Additional information given to the authors in 1973 by psychiatrists in Nanking and Shanghai is presented and correlated with reports by other recent visitors to the People's Republic of China.", "contents": "Psychiatric training and practice in the People's Republic of China. Improved political relations between the United States and the People's Republic of China have led to closer contact in many areas. The authors trace the development of psychiatric training and practice in China with emphasis on the years after 1949. Since then, China has expanded psychiatric services to meet the needs of a vast population and has developed a theoretical approach to psychiatry that emphasizes sociopolitical factors. Additional information given to the authors in 1973 by psychiatrists in Nanking and Shanghai is presented and correlated with reports by other recent visitors to the People's Republic of China."}
{"id": "PMID:1089363", "title": "Affective disorder: studies with amine precursors.", "content": "The authors assessed the clinical antidepressant effects of L-tryptophan given alone and in combination with L-dopa in 12 patients with a diagnosis of primary affective disorder; Preliminary results did not demonstrate an antidepressant response when L-dopa was combined with L-tryptophan. Also, the results did not support the catecholamine or biogenic amine hypotheses of affective disorder.", "contents": "Affective disorder: studies with amine precursors. The authors assessed the clinical antidepressant effects of L-tryptophan given alone and in combination with L-dopa in 12 patients with a diagnosis of primary affective disorder; Preliminary results did not demonstrate an antidepressant response when L-dopa was combined with L-tryptophan. Also, the results did not support the catecholamine or biogenic amine hypotheses of affective disorder."}
{"id": "PMID:1089366", "title": "Investigation of the EVI antibody in parasitic diseases other than American trypanosomiasis. An anti-skeletal muscle antibody in leishmaniasis.", "content": "The specificity of a circulating antibody observed in American trypanosomiasis and reacting with endocardium, blood vessels, and the interstitium of striated muscle (EVI factor) was evaluated in the indirect fluorescent antibody test with 60 sera from patients with malaria, leishmaniasis, echinococcosis, amebiasis, African trypanosomiasis, toxoplasmosis, and trichinosis, collected from areas where Chagas' disease is not endemic. Two sera, 1 from a patient with Plasmodium falciparum malaria and 1 from a patient with a relapse pretreatment post kala-azar dermal leishmaniasis, were positive for the EVI factor. In the leishmaniasis group, 3 of 8 sera reacted with 0ovine, murine, and human skeletal muscle. In this reaction, which differs from the EVI test, the sarcolemma and the intracellular structures were stained.", "contents": "Investigation of the EVI antibody in parasitic diseases other than American trypanosomiasis. An anti-skeletal muscle antibody in leishmaniasis. The specificity of a circulating antibody observed in American trypanosomiasis and reacting with endocardium, blood vessels, and the interstitium of striated muscle (EVI factor) was evaluated in the indirect fluorescent antibody test with 60 sera from patients with malaria, leishmaniasis, echinococcosis, amebiasis, African trypanosomiasis, toxoplasmosis, and trichinosis, collected from areas where Chagas' disease is not endemic. Two sera, 1 from a patient with Plasmodium falciparum malaria and 1 from a patient with a relapse pretreatment post kala-azar dermal leishmaniasis, were positive for the EVI factor. In the leishmaniasis group, 3 of 8 sera reacted with 0ovine, murine, and human skeletal muscle. In this reaction, which differs from the EVI test, the sarcolemma and the intracellular structures were stained."}
{"id": "PMID:1089367", "title": "Studies of in vitro infection by Trypanosoma cruzi. I. Ultrastructural studies on the invasion of macrophages and L-cells.", "content": "The interactions of Trypanosoma cruzi with L-cells, and with normal and activated macrophages in vitro were studied by ultrastructural techniques. T. cruzi actively invades cultured L-cells and uniformly destroys them. Normal macrophages could control a 1:1 (parasite to host cell) infection, but were destroyed by a 10:1 infection. BCG-activated macrophages, however, controlled a 10:1 infection but not one at a ratio  of 100:1. It appears that parasites that survive within host cells do so outside cytoplasmic vacuoles, whereas when they are relegated to host cell phagosomes they are destroyed. Culture forms of T. cruzi have several means of access into host cells. Marcrophages are better able to survive infection than are non-phagocytic cells. Finally, it is suggested that control of an experimental infection in vitro is dependent upon numbers of parasites to macrophages as well as the state of the macrophages.", "contents": "Studies of in vitro infection by Trypanosoma cruzi. I. Ultrastructural studies on the invasion of macrophages and L-cells. The interactions of Trypanosoma cruzi with L-cells, and with normal and activated macrophages in vitro were studied by ultrastructural techniques. T. cruzi actively invades cultured L-cells and uniformly destroys them. Normal macrophages could control a 1:1 (parasite to host cell) infection, but were destroyed by a 10:1 infection. BCG-activated macrophages, however, controlled a 10:1 infection but not one at a ratio  of 100:1. It appears that parasites that survive within host cells do so outside cytoplasmic vacuoles, whereas when they are relegated to host cell phagosomes they are destroyed. Culture forms of T. cruzi have several means of access into host cells. Marcrophages are better able to survive infection than are non-phagocytic cells. Finally, it is suggested that control of an experimental infection in vitro is dependent upon numbers of parasites to macrophages as well as the state of the macrophages."}
{"id": "PMID:1089368", "title": "Onchocerciasis in Guatemala. II. Microfilariae in urine, blood, and sputum after diethylcarbamazine.", "content": "Guatermalan volunteers with onchocerciasis were given a dose of diethylcarbamazine to learn if this caused migration of microfilariae of Onchocerca volvulus into urine, blood, and sputum. In 5 of the 10 volunteers, the num0ers of microfilariae in the urine increased considerably following the drug. In the same 5, relatively large numbers of microfilariae were observed in the blood and sputum specimens. Response in the remaining 5 was negligible. Four controls given a placebo did not respond. Interestingly, however, 2 control subjects had onchocercal microfilariae in their concentrated blood specimens and 3 control subjects had microfilariae in the sputum. Therefore, we believe that microfilariae, if searched for, may be found in other parts of the patient not normally associated with the infection.", "contents": "Onchocerciasis in Guatemala. II. Microfilariae in urine, blood, and sputum after diethylcarbamazine. Guatermalan volunteers with onchocerciasis were given a dose of diethylcarbamazine to learn if this caused migration of microfilariae of Onchocerca volvulus into urine, blood, and sputum. In 5 of the 10 volunteers, the num0ers of microfilariae in the urine increased considerably following the drug. In the same 5, relatively large numbers of microfilariae were observed in the blood and sputum specimens. Response in the remaining 5 was negligible. Four controls given a placebo did not respond. Interestingly, however, 2 control subjects had onchocercal microfilariae in their concentrated blood specimens and 3 control subjects had microfilariae in the sputum. Therefore, we believe that microfilariae, if searched for, may be found in other parts of the patient not normally associated with the infection."}
{"id": "PMID:1089369", "title": "Effects of pattern of ventilation on pulmonary metabolism and mechanics.", "content": "Effects of three patterns of mechanical ventilation on pulmonary mechanics, lung phospholipid and surface activity were studied in the normal closed-chest dog. The patterns were continuous mechanical ventilation with: 1) tidal volume (VT)=15 ml/kg; 2) VT=15 ml/kg with 10 cm H2O positive end-expiratory pressure (PEEP); 3) VT=50 ml/kg. THE DOGS IN EACH GROUP WERE VENTILATED FOR 24 HOURS, WITH CAREFUL ATTENTION PAID TO MAINTENANCE OF NORMAL BLOOD GASES, FLUID BALANCE, AND CARDIAC OUTPUT. The animals were sacrificed and the lungs studied to determine pressure-volume curves, dry lung weight/wet lung weight ratios, phospholpid contents and surface activities. The results were compared with control values in acutely sacrificed unventilated dogs. No significant change from controls was found with any pattern of ventilation employed with the exception of the tendency for lungs ventilated with PEEP to retain fluid (decreased dry lung weight/wet lung weight ratio).", "contents": "Effects of pattern of ventilation on pulmonary metabolism and mechanics. Effects of three patterns of mechanical ventilation on pulmonary mechanics, lung phospholipid and surface activity were studied in the normal closed-chest dog. The patterns were continuous mechanical ventilation with: 1) tidal volume (VT)=15 ml/kg; 2) VT=15 ml/kg with 10 cm H2O positive end-expiratory pressure (PEEP); 3) VT=50 ml/kg. THE DOGS IN EACH GROUP WERE VENTILATED FOR 24 HOURS, WITH CAREFUL ATTENTION PAID TO MAINTENANCE OF NORMAL BLOOD GASES, FLUID BALANCE, AND CARDIAC OUTPUT. The animals were sacrificed and the lungs studied to determine pressure-volume curves, dry lung weight/wet lung weight ratios, phospholpid contents and surface activities. The results were compared with control values in acutely sacrificed unventilated dogs. No significant change from controls was found with any pattern of ventilation employed with the exception of the tendency for lungs ventilated with PEEP to retain fluid (decreased dry lung weight/wet lung weight ratio)."}
{"id": "PMID:1089370", "title": "Intestinal infusion of endotoxin in adrenalectomized calves.", "content": "Four adrenalectomized calves, 3 to 4 months old, were allowed to develop adrenocorticosteroid insufficiency and then were given 50 mg of Escherichia coli endotoxin by infusion via cannula into the duodenum. Several physiologic variables were studied to detect changes indicative of endotoxemia. It is concluded that adrenocorticosteroid insufficiency in calves is not an independent factor allowing an intestinal source of endotoxin to cause the development of clinical signs and physiologic changes characteristic of endotoxemia.", "contents": "Intestinal infusion of endotoxin in adrenalectomized calves. Four adrenalectomized calves, 3 to 4 months old, were allowed to develop adrenocorticosteroid insufficiency and then were given 50 mg of Escherichia coli endotoxin by infusion via cannula into the duodenum. Several physiologic variables were studied to detect changes indicative of endotoxemia. It is concluded that adrenocorticosteroid insufficiency in calves is not an independent factor allowing an intestinal source of endotoxin to cause the development of clinical signs and physiologic changes characteristic of endotoxemia."}
{"id": "PMID:1089371", "title": "A rapid batch method for the production of specific fluorescein isothiocyanate-labeled globulins to Dirofilaria immitis microfilaria.", "content": "A rapid, reproducible method for the production of specifically labeled anti-Dirofilaria immitis microfilaria (MFF) globulins (MAG) has been devised. These labeled antibodies have made the detection of D immitis in serum possible by a fluorescent-antibody technique.", "contents": "A rapid batch method for the production of specific fluorescein isothiocyanate-labeled globulins to Dirofilaria immitis microfilaria. A rapid, reproducible method for the production of specifically labeled anti-Dirofilaria immitis microfilaria (MFF) globulins (MAG) has been devised. These labeled antibodies have made the detection of D immitis in serum possible by a fluorescent-antibody technique."}
{"id": "PMID:1089372", "title": "Relative immunogenicity of streptomycin-susceptible and -resistant strains of BCG. II. Effect of the route of inoculation on growth and immunogenicity.", "content": "Normal CD-1 mice were infected intravenously, subcutaneously, or aerogenically with live bacille Calmette Guerin (BCG) Tice or BCG streptomycin resistant (SM-res) and growth of the organisms in the footpad, the draining popliteal lymph node, the blood, lung, liver, and spleen was followed for as long as 50 days. The vaccinated mice were then challenged on day 50 with 10-5 viable Mycobacterium tuberculosis Erdman organisms introduced intravenously or subcutaneously. The growth of the Erdman challenge was followed in the appropriate organs for the next 20 days. Measurement of tuberculin hypersensitivity was carried out by footpad tests. The BCG Tice introduced aerogenically or subcutaniously into normal mice induced degrees of antituberculous resistance equivalent to those seen earlier in intravenously infected mice. The BCG SM-res was still nonimmunogenic when introduced subcutaneously or by the aerogenic route. Suspension of the organisms in sterile mineral oil before their injection into the footpad slowed their rate of inactivation and marginally increased the immune response seen later in the host. Introduction of BCG SM-res into T-cell depleted mice by the 3 inoculation routes was associated with no marked improvement in the survival of this organism in vivo, suggesting that BCG SM-res is inactivated in vivo by a nonimmunologically mediated mechanism.", "contents": "Relative immunogenicity of streptomycin-susceptible and -resistant strains of BCG. II. Effect of the route of inoculation on growth and immunogenicity. Normal CD-1 mice were infected intravenously, subcutaneously, or aerogenically with live bacille Calmette Guerin (BCG) Tice or BCG streptomycin resistant (SM-res) and growth of the organisms in the footpad, the draining popliteal lymph node, the blood, lung, liver, and spleen was followed for as long as 50 days. The vaccinated mice were then challenged on day 50 with 10-5 viable Mycobacterium tuberculosis Erdman organisms introduced intravenously or subcutaneously. The growth of the Erdman challenge was followed in the appropriate organs for the next 20 days. Measurement of tuberculin hypersensitivity was carried out by footpad tests. The BCG Tice introduced aerogenically or subcutaniously into normal mice induced degrees of antituberculous resistance equivalent to those seen earlier in intravenously infected mice. The BCG SM-res was still nonimmunogenic when introduced subcutaneously or by the aerogenic route. Suspension of the organisms in sterile mineral oil before their injection into the footpad slowed their rate of inactivation and marginally increased the immune response seen later in the host. Introduction of BCG SM-res into T-cell depleted mice by the 3 inoculation routes was associated with no marked improvement in the survival of this organism in vivo, suggesting that BCG SM-res is inactivated in vivo by a nonimmunologically mediated mechanism."}
{"id": "PMID:1089373", "title": "Bronchofiberscopy.", "content": "Probably no other diagnostic interpretive technique has revolutionized pulmonary medical practice in so short a time as flexible bronchofiberscopy. This state of the art review deals with flexible bronchofiberscopy, as well as comparisons to rigid bronchoscopic techniques. Historic development, specifications, indications, contraindications, sterilization of the instrument, and complications of these methods are discussed. Accessory procedures to bronchofiberscopy including biopsy, brushing, lavage, foreign body extraction, selective bacterial cultures, and research applications are considered. Finally, recommendations are made as to the training of a physician who performs bronchofiberscopy.", "contents": "Bronchofiberscopy. Probably no other diagnostic interpretive technique has revolutionized pulmonary medical practice in so short a time as flexible bronchofiberscopy. This state of the art review deals with flexible bronchofiberscopy, as well as comparisons to rigid bronchoscopic techniques. Historic development, specifications, indications, contraindications, sterilization of the instrument, and complications of these methods are discussed. Accessory procedures to bronchofiberscopy including biopsy, brushing, lavage, foreign body extraction, selective bacterial cultures, and research applications are considered. Finally, recommendations are made as to the training of a physician who performs bronchofiberscopy."}
{"id": "PMID:1089374", "title": "Distribution of sodium and potassium in chronic obstructive pulmonary disease.", "content": "The body electrolyte composition of 34 patients with severe chronic obstructive pulmonary disease and without complications or medications known to influence body sodium or potassium was studied. Exchangeable sodium, exchangeable potassium, extracellular water, and total body water were measured using radioisotope dilution techniques. From these values and serum sodium and potassium levels, the intracellular water, intracellular concentrations of sodium and potassium, and factors to estimate lean body mass were calculated. Normal values were predicted for factors that vary with weight, age, and sex--exchangeable sodium, exchangeable potassium, total body water, extracellular water, intracellular water, and estimates of lean body mass--for each subject using regression equations that took into account the variables. For values that do not vary with weight or age--residual sodium and intracellular concentration of potassium--comparisons were made with values from our control subjects. The patients with chronic obstructive pulmonary disease showed a slower rate of potassium exchange than the control subjects, requiring at least 48 hours. There was also an increase in residual sodium composed of intracellular sodium and exchangeable bone sodium. The possibilities that the abnormalities could be ascribed to congestive heart failure, loss of lean tissue, or to a slowly functioning sodium pump were considered.", "contents": "Distribution of sodium and potassium in chronic obstructive pulmonary disease. The body electrolyte composition of 34 patients with severe chronic obstructive pulmonary disease and without complications or medications known to influence body sodium or potassium was studied. Exchangeable sodium, exchangeable potassium, extracellular water, and total body water were measured using radioisotope dilution techniques. From these values and serum sodium and potassium levels, the intracellular water, intracellular concentrations of sodium and potassium, and factors to estimate lean body mass were calculated. Normal values were predicted for factors that vary with weight, age, and sex--exchangeable sodium, exchangeable potassium, total body water, extracellular water, intracellular water, and estimates of lean body mass--for each subject using regression equations that took into account the variables. For values that do not vary with weight or age--residual sodium and intracellular concentration of potassium--comparisons were made with values from our control subjects. The patients with chronic obstructive pulmonary disease showed a slower rate of potassium exchange than the control subjects, requiring at least 48 hours. There was also an increase in residual sodium composed of intracellular sodium and exchangeable bone sodium. The possibilities that the abnormalities could be ascribed to congestive heart failure, loss of lean tissue, or to a slowly functioning sodium pump were considered."}
{"id": "PMID:1089375", "title": "Neural regulation of respiration.", "content": "The main respiratory muscles are under both voluntary and involuntary (automatic) control. These two control systems come from separate sites in the CNS and have separate descending pathways; the final integration of these outputs occurs at segmental levels in the cord. Voluntary control arises from the motor and premotor cortex and descends in the cord in the corticospinal tract. Involuntary control is mediated by both rhythmic and nonrhythmic systems located in the brainstem. Recent studies have associated the classic respiratory centers with specific nuclei in the brainstem. The pneumotaxic center is located in the nucleus parabrachialis, and the medullary respiratory centers are located in the vicinity of the nucleus of the solitary tract (dorsal respiratory group) and the nuclei ambiguus and retroambigualis (ventral respiratory group). Most axons from the medullary nuclei cross in the medulla and descend in the ventral and lateral columns to segmental levels. The classic medullary respiratory centers described by Pitts have been shown to be the site of origin of tonically firing long reticulospinal axons that descend in the ventral and lateral columns. This system is thought to provide a nonrhythmic involuntary biasing of the membrane potential of respiratory motoneurons in the cord. The site of generation of eupnic breathing and the mechanism of rhythm generation remain unknown. However, recent studies indicate that reciprocal inhibition between populations of inspiratory and expiratory cells (bistable oscillator model) does not occur in the medulla; rather we suggest that inhibitory phasing of inspiratory cells generates inspiratory rhythm, and periodic inhibition of tonically active expiratory neurons results in respiratory rhythm. We suggest that the inhibitory phasing occurs in the dorsal respiratory group, which is also the site of integration of respiratory afferents. The main site of integration of the voluntary and involuntary control systems is the cord where the respiratory motoneuron output is determined by descending information from these systems, as well as with intrasegmental and intersegmental reflexes. Separation of the voluntary and involuntary control systems also occurs in man and discrete lesions may interrupt one system without significant alteration of the other.", "contents": "Neural regulation of respiration. The main respiratory muscles are under both voluntary and involuntary (automatic) control. These two control systems come from separate sites in the CNS and have separate descending pathways; the final integration of these outputs occurs at segmental levels in the cord. Voluntary control arises from the motor and premotor cortex and descends in the cord in the corticospinal tract. Involuntary control is mediated by both rhythmic and nonrhythmic systems located in the brainstem. Recent studies have associated the classic respiratory centers with specific nuclei in the brainstem. The pneumotaxic center is located in the nucleus parabrachialis, and the medullary respiratory centers are located in the vicinity of the nucleus of the solitary tract (dorsal respiratory group) and the nuclei ambiguus and retroambigualis (ventral respiratory group). Most axons from the medullary nuclei cross in the medulla and descend in the ventral and lateral columns to segmental levels. The classic medullary respiratory centers described by Pitts have been shown to be the site of origin of tonically firing long reticulospinal axons that descend in the ventral and lateral columns. This system is thought to provide a nonrhythmic involuntary biasing of the membrane potential of respiratory motoneurons in the cord. The site of generation of eupnic breathing and the mechanism of rhythm generation remain unknown. However, recent studies indicate that reciprocal inhibition between populations of inspiratory and expiratory cells (bistable oscillator model) does not occur in the medulla; rather we suggest that inhibitory phasing of inspiratory cells generates inspiratory rhythm, and periodic inhibition of tonically active expiratory neurons results in respiratory rhythm. We suggest that the inhibitory phasing occurs in the dorsal respiratory group, which is also the site of integration of respiratory afferents. The main site of integration of the voluntary and involuntary control systems is the cord where the respiratory motoneuron output is determined by descending information from these systems, as well as with intrasegmental and intersegmental reflexes. Separation of the voluntary and involuntary control systems also occurs in man and discrete lesions may interrupt one system without significant alteration of the other."}
{"id": "PMID:1089377", "title": "Allergic bronchopulmonary aspergillosis: evidence of limited tissue invasion.", "content": "The cause of granulomatous parenchymal reaction in allergic bronchopulmonary aspergillosis has been attributed to a hypersensitivity reaction. A case is reported with Aspergillus fumigatus identified in lung tissue, suggesting that the pathologic changes in some cases can be due to limited parenchymal colonization.", "contents": "Allergic bronchopulmonary aspergillosis: evidence of limited tissue invasion. The cause of granulomatous parenchymal reaction in allergic bronchopulmonary aspergillosis has been attributed to a hypersensitivity reaction. A case is reported with Aspergillus fumigatus identified in lung tissue, suggesting that the pathologic changes in some cases can be due to limited parenchymal colonization."}
{"id": "PMID:1089380", "title": "Blood--its derivatives and its problems--factor VIII.", "content": "Unfortunately, all of the problems of the hemophiliac have not been solved by the availability of concentrated factor VIII products. Patients still are faced with the crippling effects of arthritis, problems with employment, problems with ignorance (both medical and lay), and an increased risk of premature death even in a sophisticated, treatment-oriented, community (Table 3). It can only be hoped that we can solve the problems of hepatitis transmission, availability, and economics so that concentrated forms of factor VIII can be made available to all patients with hemophilia. It seems appropriate to suggest that our severely affected patients should be placed on some prophylactic programs, since this would ease most of the long-term psychologic and physical disabilities common to this disease.", "contents": "Blood--its derivatives and its problems--factor VIII. Unfortunately, all of the problems of the hemophiliac have not been solved by the availability of concentrated factor VIII products. Patients still are faced with the crippling effects of arthritis, problems with employment, problems with ignorance (both medical and lay), and an increased risk of premature death even in a sophisticated, treatment-oriented, community (Table 3). It can only be hoped that we can solve the problems of hepatitis transmission, availability, and economics so that concentrated forms of factor VIII can be made available to all patients with hemophilia. It seems appropriate to suggest that our severely affected patients should be placed on some prophylactic programs, since this would ease most of the long-term psychologic and physical disabilities common to this disease."}
{"id": "PMID:1089384", "title": "Detachment of Descemet's membrane from grafts following wound separation: light and scanning electron microscopic study.", "content": "Examination of 4 specimens of failed grafts following wound rupture shows that separation of Descement's membrane from the periphery of the graft in the area of dehiscence is followed by morphological changes in endotelial cells and by the development of fibrous membranes. Defective wound healing after repair is probably caused by persistent edema of graft stroma. With sudden decompression of the globe, rupture and total detachment of Descemet's membrane may occur. Changes in Descemet's membrane and eventual failure of the graft may occur mostly on the basis of anatomical changes in the endothelium of the graft and not necessarily by inflammation which was not present in these 4 specimens.", "contents": "Detachment of Descemet's membrane from grafts following wound separation: light and scanning electron microscopic study. Examination of 4 specimens of failed grafts following wound rupture shows that separation of Descement's membrane from the periphery of the graft in the area of dehiscence is followed by morphological changes in endotelial cells and by the development of fibrous membranes. Defective wound healing after repair is probably caused by persistent edema of graft stroma. With sudden decompression of the globe, rupture and total detachment of Descemet's membrane may occur. Changes in Descemet's membrane and eventual failure of the graft may occur mostly on the basis of anatomical changes in the endothelium of the graft and not necessarily by inflammation which was not present in these 4 specimens."}
{"id": "PMID:1089385", "title": "Anterior segment fluorescein angiography: a simple modification of the Zeiss stereo slit lamp camera.", "content": "The addition of a small metal housing containing a rechargeable flash to a Zeiss stereo slit lamp camera provides a simple, inexpensive method for obtaining excellent quality anterior segment fluorescein angiograms. By placing a splice in the slit illumination cord the fluorescein flash unit can be hooked up to be existing Zeiss power pack. The easy insertion of the proper excitation and barrier filters completes the temporary conversion. Utilizing this equipment, rapid sequence photographs of high resolution can be achieved. In addition, stereophotography, variable magnification, color photography and a simultaneous combination of fluorescein and infrared angiography for pigmented lesions are possible.", "contents": "Anterior segment fluorescein angiography: a simple modification of the Zeiss stereo slit lamp camera. The addition of a small metal housing containing a rechargeable flash to a Zeiss stereo slit lamp camera provides a simple, inexpensive method for obtaining excellent quality anterior segment fluorescein angiograms. By placing a splice in the slit illumination cord the fluorescein flash unit can be hooked up to be existing Zeiss power pack. The easy insertion of the proper excitation and barrier filters completes the temporary conversion. Utilizing this equipment, rapid sequence photographs of high resolution can be achieved. In addition, stereophotography, variable magnification, color photography and a simultaneous combination of fluorescein and infrared angiography for pigmented lesions are possible."}
{"id": "PMID:1089392", "title": "A gastroplasty for short esophagus and reflux esophagitis: experimental and clinical studies.", "content": "A safe, simple, effective gastroplasty for short esophagus with reflux esophagitis is described. It has been evaluated in dogs for up to three years with flexible fiberesophagoscopy, esophagrams and intraluminal pressure studies. Successful clinical experience has been encouraging.", "contents": "A gastroplasty for short esophagus and reflux esophagitis: experimental and clinical studies. A safe, simple, effective gastroplasty for short esophagus with reflux esophagitis is described. It has been evaluated in dogs for up to three years with flexible fiberesophagoscopy, esophagrams and intraluminal pressure studies. Successful clinical experience has been encouraging."}
{"id": "PMID:1089393", "title": "Roux-en-Y hepaticojejunostomy: a reappraisal of its indications and results.", "content": "A critical evaluation is made of 131 patients submitted to choledocho or hepaticojejunostomy. The main indications for hepaticojejunostomy were iatrogenic strictures of CBD (60 patients), and choledocholithiasis with markedly dilated duct (41 patients). The overall mortality rate was 4% representing principally renal hepatic failure, bile peritonitis and bleeding. The complications following hepaticojejunostomy included only in one case biliary fistula which required reoperation. The long-term results of 80 patients available for a followup study were as follows: 63 patients (78.7%) were symptom-free at 2-13 years followup; 8 patients had brief episodes of cholangitis which responded to antibiotic and corticosteroid treatment; 9 patients required reoperation for stricture of anastomosis. These overall results are a strong argument for hepaticojejunostomy which, compared with choledochoduodenostomy, avoids the hazards of the so-called sump syndrome and of the reflux of enteric contents in the CBD. An increased incidence of peptic ulcer disease in the patients submitted to hepaticojejunostomy was not observed. In very high strictures and in reinterventions anastomosis between left hepatic duct and Roux-en-Y jejunal limb was carried out. The results achieved with this technique, which was performed in 26 patients, were about the same following hepaticojejunostomy.", "contents": "Roux-en-Y hepaticojejunostomy: a reappraisal of its indications and results. A critical evaluation is made of 131 patients submitted to choledocho or hepaticojejunostomy. The main indications for hepaticojejunostomy were iatrogenic strictures of CBD (60 patients), and choledocholithiasis with markedly dilated duct (41 patients). The overall mortality rate was 4% representing principally renal hepatic failure, bile peritonitis and bleeding. The complications following hepaticojejunostomy included only in one case biliary fistula which required reoperation. The long-term results of 80 patients available for a followup study were as follows: 63 patients (78.7%) were symptom-free at 2-13 years followup; 8 patients had brief episodes of cholangitis which responded to antibiotic and corticosteroid treatment; 9 patients required reoperation for stricture of anastomosis. These overall results are a strong argument for hepaticojejunostomy which, compared with choledochoduodenostomy, avoids the hazards of the so-called sump syndrome and of the reflux of enteric contents in the CBD. An increased incidence of peptic ulcer disease in the patients submitted to hepaticojejunostomy was not observed. In very high strictures and in reinterventions anastomosis between left hepatic duct and Roux-en-Y jejunal limb was carried out. The results achieved with this technique, which was performed in 26 patients, were about the same following hepaticojejunostomy."}
{"id": "PMID:1089388", "title": "Permanent deafness associated with furosemide administration.", "content": "Six cases of hearing impairment have been presented. Furosemide was administered to all of these patients at the time of the alleged onset of the deafness. None of the known or suspected causes of deafness occurring in transplant patients was evident in these cases. With the experimental evidence that furosemide does induce stria damage and the absence of other recognized causes of deafness in this series of patients, furosemide must be considered the etiologic agent responsible for the permanent sensorineural hearing loss. A characteristic audiometric pattern of hearing impairment emerged and a possible explanation for these features has been given. Certain precautionary steps in the use of ototoxic drugs are suggested.", "contents": "Permanent deafness associated with furosemide administration. Six cases of hearing impairment have been presented. Furosemide was administered to all of these patients at the time of the alleged onset of the deafness. None of the known or suspected causes of deafness occurring in transplant patients was evident in these cases. With the experimental evidence that furosemide does induce stria damage and the absence of other recognized causes of deafness in this series of patients, furosemide must be considered the etiologic agent responsible for the permanent sensorineural hearing loss. A characteristic audiometric pattern of hearing impairment emerged and a possible explanation for these features has been given. Certain precautionary steps in the use of ototoxic drugs are suggested."}
{"id": "PMID:1089394", "title": "The precision of a special purpose analog computer in clinical cardiac output determination.", "content": "Three hundred dye-dilution curves taken during our first year of clinical experience with the Waters CO-4 cardiac output computer were analyzed to estimate the errors involved in its use. Provided that calibration is accurate and 5.0 mg of dye are injected for each curve, then the percentage standard deviation of measurement using this computer is about 8.7%. Included in this are the errors inherent in the computer, errors due to baseline drift, errors in the injection of dye and acutal variation of cardiac output over a series of successive determinations. The size of this error is comparable to that involved in manual calculation. The mean value of five successive curves will be within 10% of the real value in 99 cases out of 100. Advances in methodology and equipment are discussed which make calibration simpler and more accurate, and which should also improve the quality of computer determination. A list of suggestions is given to minimize the errors involved in the clinical use of this equipment.", "contents": "The precision of a special purpose analog computer in clinical cardiac output determination. Three hundred dye-dilution curves taken during our first year of clinical experience with the Waters CO-4 cardiac output computer were analyzed to estimate the errors involved in its use. Provided that calibration is accurate and 5.0 mg of dye are injected for each curve, then the percentage standard deviation of measurement using this computer is about 8.7%. Included in this are the errors inherent in the computer, errors due to baseline drift, errors in the injection of dye and acutal variation of cardiac output over a series of successive determinations. The size of this error is comparable to that involved in manual calculation. The mean value of five successive curves will be within 10% of the real value in 99 cases out of 100. Advances in methodology and equipment are discussed which make calibration simpler and more accurate, and which should also improve the quality of computer determination. A list of suggestions is given to minimize the errors involved in the clinical use of this equipment."}
{"id": "PMID:1089389", "title": "Changes in the lymphocyte cytoplasmic refractive index following typhoid vaccination.", "content": "The refractive index of the circulating blood lymphocytes (LCRI) of healthy young adults vaccinated with typhoid vaccine has been measured by immersion refractometry and phase contrast microscopy. A rise in the LCRI preceeds the rise in the antibody titre by two to three days. A more pronounced rise also occurred in one patient prior to a rejection crises following a kidney transplant.", "contents": "Changes in the lymphocyte cytoplasmic refractive index following typhoid vaccination. The refractive index of the circulating blood lymphocytes (LCRI) of healthy young adults vaccinated with typhoid vaccine has been measured by immersion refractometry and phase contrast microscopy. A rise in the LCRI preceeds the rise in the antibody titre by two to three days. A more pronounced rise also occurred in one patient prior to a rejection crises following a kidney transplant."}
{"id": "PMID:1089387", "title": "Local anesthesia in otolaryngology. A re-evaluation.", "content": "Almost a half century following attempts to ban its use, cocaine remains at the pinnacle of topical anesthesia in otolaryngology. To understand how nonaddicting synthetic substitutes such as procaine, dibucaine, tetracaine and lidocaine have not totally supplanted cocaine, requires an in-depth analysis of its unique pharmological properties, untoward effects and potential substitutes. Almost all of the reported cocaine deaths occurred after subcutaneous injection; when used topically, cocaine's toxicity has been confined to an occasional reaction. Certain variables under physician control may be manipulated to reduce the chance of reaction to a minimum. For example, intermittent application of a particular dosage results in lower blood levels, and allowing sufficient time between doses reduces the amount necessary to obtain the desired anesthesia. If total dosage is kept below 200 mg there are few reactions. A singular advantage of cocaine over other topical anesthetics is its inherent ability to cause vasoconstriction, thus retarding its own absorption. The addition of a topical vasoconstrictor such as epinephrine is thus redundant, and may actually be harmful as cocaine sensitizes the patient to exogenous epinephrine. Finally, the usual preoperative dosages of barbiturates are entirely inadequate to prevent or treat cocaine reactions. Why, then, have synthetic local anesthetics not replaced cocaine? Inherent differences in topical effectiveness, duration of anesthesia and toxicity provide the answer. Of other local anesthetics possessing topical effectiveness tetracaine is about six times more toxic than cocaine. Dibucaine is as toxic as tetracaine, and lidocaine, while relatively nontoxic, provides only a 15 minute duration of topical anesthesia. A review of cocaine and its potential substitutes thus leads to the conclusion that cocaine is still a vital and necessary instrument in the otolaryngologist's armamentarium, singularly providing excellent topical anesthesia of usable duration, vasoconstriction, and shrinkage of mucous membranes, all with a quite acceptable margin of safety.", "contents": "Local anesthesia in otolaryngology. A re-evaluation. Almost a half century following attempts to ban its use, cocaine remains at the pinnacle of topical anesthesia in otolaryngology. To understand how nonaddicting synthetic substitutes such as procaine, dibucaine, tetracaine and lidocaine have not totally supplanted cocaine, requires an in-depth analysis of its unique pharmological properties, untoward effects and potential substitutes. Almost all of the reported cocaine deaths occurred after subcutaneous injection; when used topically, cocaine's toxicity has been confined to an occasional reaction. Certain variables under physician control may be manipulated to reduce the chance of reaction to a minimum. For example, intermittent application of a particular dosage results in lower blood levels, and allowing sufficient time between doses reduces the amount necessary to obtain the desired anesthesia. If total dosage is kept below 200 mg there are few reactions. A singular advantage of cocaine over other topical anesthetics is its inherent ability to cause vasoconstriction, thus retarding its own absorption. The addition of a topical vasoconstrictor such as epinephrine is thus redundant, and may actually be harmful as cocaine sensitizes the patient to exogenous epinephrine. Finally, the usual preoperative dosages of barbiturates are entirely inadequate to prevent or treat cocaine reactions. Why, then, have synthetic local anesthetics not replaced cocaine? Inherent differences in topical effectiveness, duration of anesthesia and toxicity provide the answer. Of other local anesthetics possessing topical effectiveness tetracaine is about six times more toxic than cocaine. Dibucaine is as toxic as tetracaine, and lidocaine, while relatively nontoxic, provides only a 15 minute duration of topical anesthesia. A review of cocaine and its potential substitutes thus leads to the conclusion that cocaine is still a vital and necessary instrument in the otolaryngologist's armamentarium, singularly providing excellent topical anesthesia of usable duration, vasoconstriction, and shrinkage of mucous membranes, all with a quite acceptable margin of safety."}
{"id": "PMID:1089390", "title": "Testicular changes associated with the initiation of spermatogenesis.", "content": "Testicular changes preceding and accompanying the initiation of spermatogenesis are reviewed. The complex cellular changes occurring in the developing testis are found to be particularly well defined with the use of electron microscopy. The findings suggest that electron microscopic examination may be useful in the evaluation of male infertility, especially in those cases in which clinical and laboratory findings are negative or inconclusive. The amount of tissue required is minimal and can be taken as part of the regular testicular biopsy. The additional information provided by electron microscopy can be used to clarify the observations made on routine light microscopic study and may also lead to better understanding of the pathologic changes occurring in some of the less well understood forms of male infertility.", "contents": "Testicular changes associated with the initiation of spermatogenesis. Testicular changes preceding and accompanying the initiation of spermatogenesis are reviewed. The complex cellular changes occurring in the developing testis are found to be particularly well defined with the use of electron microscopy. The findings suggest that electron microscopic examination may be useful in the evaluation of male infertility, especially in those cases in which clinical and laboratory findings are negative or inconclusive. The amount of tissue required is minimal and can be taken as part of the regular testicular biopsy. The additional information provided by electron microscopy can be used to clarify the observations made on routine light microscopic study and may also lead to better understanding of the pathologic changes occurring in some of the less well understood forms of male infertility."}
{"id": "PMID:1089395", "title": "Improvement in lower esophageal sphincter pressure following surgery for complicated gastroesophageal reflux.", "content": "A comparison was made of the pre- and postoperative lower esophageal sphincter (LES) pressures in nine patients undergoing a posterior gastropexy for complicated gastroesophageal reflux. LES pressure was increased from 4.4 plus or minus 0.4 mm Hg to 13.9 plus or minus 0.5 mm Hg following surgery (p less than .01). The ratio of the change in LES pressure compared to the change in gastric pressure during increases in intra-abdominal pressure delta S/delta G, was 0.59 plus or minus 0.05 preoperatively and 0.94 plus or minus .01 postoperatively (p less than .01). All patients were asymptomatic after surgery. Both the resting LES pressure and the S/G ratio following surgery were significantly less than the comparable values obtained in an age-matched control population (p less than .01). These studies suggest that the clinical improvement following surgery for gastroesophageal reflux may be due to the increase in resting LES pressure and the improved response of the LES to increased intra-abdominal pressure.", "contents": "Improvement in lower esophageal sphincter pressure following surgery for complicated gastroesophageal reflux. A comparison was made of the pre- and postoperative lower esophageal sphincter (LES) pressures in nine patients undergoing a posterior gastropexy for complicated gastroesophageal reflux. LES pressure was increased from 4.4 plus or minus 0.4 mm Hg to 13.9 plus or minus 0.5 mm Hg following surgery (p less than .01). The ratio of the change in LES pressure compared to the change in gastric pressure during increases in intra-abdominal pressure delta S/delta G, was 0.59 plus or minus 0.05 preoperatively and 0.94 plus or minus .01 postoperatively (p less than .01). All patients were asymptomatic after surgery. Both the resting LES pressure and the S/G ratio following surgery were significantly less than the comparable values obtained in an age-matched control population (p less than .01). These studies suggest that the clinical improvement following surgery for gastroesophageal reflux may be due to the increase in resting LES pressure and the improved response of the LES to increased intra-abdominal pressure."}
{"id": "PMID:1089396", "title": "Cellular kinetics in multiple myeloma. A new approach to staging and treatment.", "content": "Previous studies of myeloma immunoglobulin synthesis and tumor cell number, using a marker kinetic approach, have increased knowledge about body bruden of tumor cells, kinetics of myeloma cell growth, and response to chemotherapy. On the basis of these observations, we have developed a clinical staging system that permits accurate estimation of total myeloma cell number in patients who have not had specialized immunosynthetic measurements. We have incorporated this clinical staging into a nationwide time-sharing computer network called the Myeloma Study System (MSS). With the MSS we are able to establish a file for each patient, keep track of changes in tumor cell number with treatment, and evaluate toxicity. This resource should afford an excellent test of the value of cellular kinetics for the planning and uniform evaluation of cancer chemotherapy.", "contents": "Cellular kinetics in multiple myeloma. A new approach to staging and treatment. Previous studies of myeloma immunoglobulin synthesis and tumor cell number, using a marker kinetic approach, have increased knowledge about body bruden of tumor cells, kinetics of myeloma cell growth, and response to chemotherapy. On the basis of these observations, we have developed a clinical staging system that permits accurate estimation of total myeloma cell number in patients who have not had specialized immunosynthetic measurements. We have incorporated this clinical staging into a nationwide time-sharing computer network called the Myeloma Study System (MSS). With the MSS we are able to establish a file for each patient, keep track of changes in tumor cell number with treatment, and evaluate toxicity. This resource should afford an excellent test of the value of cellular kinetics for the planning and uniform evaluation of cancer chemotherapy."}
{"id": "PMID:1089397", "title": "Monoclonal gammopathy in lymphoma.", "content": "Serum protein electrophoresis was performed in 68% of 1,682 consecutive patients with lymphoma. Of 400 patients with chronic lymphocytic leukemia and lymphocytic lymphoma, 2.3% had an IgG peak, a frequency significantly higher than that found in normal individuals of comparable age. IgM peaks occurred in 4.5% of patients with lymphomas characterized by diffuse histologic infiltration of lymph nodes. This frequency was about 100 times greater than predicted and indicated that most IgM peaks detected by electrophoresis are produced by lymphomas. Clinical responses to therapy were associated with reductions of tumor mass usually ranging from 10% to 50% of the pretreatment level. A much greater magnitude of tumor reduction is needed to improve remission duration for patients with chronic lymphocytic leukemia and lymphocytic lymphoma.", "contents": "Monoclonal gammopathy in lymphoma. Serum protein electrophoresis was performed in 68% of 1,682 consecutive patients with lymphoma. Of 400 patients with chronic lymphocytic leukemia and lymphocytic lymphoma, 2.3% had an IgG peak, a frequency significantly higher than that found in normal individuals of comparable age. IgM peaks occurred in 4.5% of patients with lymphomas characterized by diffuse histologic infiltration of lymph nodes. This frequency was about 100 times greater than predicted and indicated that most IgM peaks detected by electrophoresis are produced by lymphomas. Clinical responses to therapy were associated with reductions of tumor mass usually ranging from 10% to 50% of the pretreatment level. A much greater magnitude of tumor reduction is needed to improve remission duration for patients with chronic lymphocytic leukemia and lymphocytic lymphoma."}
{"id": "PMID:1089398", "title": "Immunochemical, clical, and pathological features of alpha-chain disease.", "content": "Making the diagnosis of alpha-chain disease in the laboratory is difficult. Immunologic and structural characteristics of the abnormal protein were shown in results of cellular studies. There are clinicopathological features associated with the protein abnormality. The peculiar geographic origin of patients affected with alpha-chain disease and the possible reversibility of the hyperplastic process at its early stage lead to some considerations of cause.", "contents": "Immunochemical, clical, and pathological features of alpha-chain disease. Making the diagnosis of alpha-chain disease in the laboratory is difficult. Immunologic and structural characteristics of the abnormal protein were shown in results of cellular studies. There are clinicopathological features associated with the protein abnormality. The peculiar geographic origin of patients affected with alpha-chain disease and the possible reversibility of the hyperplastic process at its early stage lead to some considerations of cause."}
{"id": "PMID:1089399", "title": "Antidepressant response to tricyclics and urinary MHPG in unipolar patients. Clinical response to imipramine or amitriptyline.", "content": "The urinary excretion of the norepinephrine metabolite 3-methoxy-4-hydroxyphenylglycol (MHPG) was measured in unipolar depressed patients before and during the fourth week of treatment with either imipramine hydrocloride or amitriptyline hydroxhloride. On the basis of strict rating criteria, 24 patients were selected as either unequivocal responders or nonresponders. In the imipramine group the mean pretreatment MHPG was significantly lower in the nine responders in the seven nonresponders; the converse was found with the amitriptyline patients. Of particular interest is that there was no overlap in individual values between the responders and nonresponders to either drug. Treatment with eigher imipramine or amitriptyline was associated with a significant decrease in MHPG excretion, which was independent of clinical response.", "contents": "Antidepressant response to tricyclics and urinary MHPG in unipolar patients. Clinical response to imipramine or amitriptyline. The urinary excretion of the norepinephrine metabolite 3-methoxy-4-hydroxyphenylglycol (MHPG) was measured in unipolar depressed patients before and during the fourth week of treatment with either imipramine hydrocloride or amitriptyline hydroxhloride. On the basis of strict rating criteria, 24 patients were selected as either unequivocal responders or nonresponders. In the imipramine group the mean pretreatment MHPG was significantly lower in the nine responders in the seven nonresponders; the converse was found with the amitriptyline patients. Of particular interest is that there was no overlap in individual values between the responders and nonresponders to either drug. Treatment with eigher imipramine or amitriptyline was associated with a significant decrease in MHPG excretion, which was independent of clinical response."}
{"id": "PMID:1089400", "title": "Amine precursors and depression.", "content": "The amino acid precursors, levodopa and L-tryptophan, were given to a group of hospitalized depressed patients in a double-blind placebo controlled study. Relatively large doses were not associated with sufficient clinical improvement to allow the patients to leave the hospital. Previous studies using the precursor-load strategy have produced conflicting findings on the use of these compounds for depressed patients.", "contents": "Amine precursors and depression. The amino acid precursors, levodopa and L-tryptophan, were given to a group of hospitalized depressed patients in a double-blind placebo controlled study. Relatively large doses were not associated with sufficient clinical improvement to allow the patients to leave the hospital. Previous studies using the precursor-load strategy have produced conflicting findings on the use of these compounds for depressed patients."}
{"id": "PMID:1089401", "title": "Psychoactive drugs in mania. A controlled comparison of lithium carbonate, chlorpromazine, and haloperidol.", "content": "Lithium carbonate, haloperidol, and chlorpromazine hydrochloride were compared in a double-blind controlled study with severely ill hospitalized manics. Lithium carbonate and haloperidol produced a highly significant improvement of manic symptoms without sedation. Although producing considerable sedation, chlorpromazine did little to alter the underlying mania qualitatively. Qualitative differences between lithium carbonate and haloperidol indicate that, while haloperidol has a more dramatically rapid impact on behavior-motor activity, lithium carbonate acted more evenly on the entire manic picture, with total normalization realized during active treatment. The majority of lithium carbonate-treated patients met discharge criteria at study termination, but not the patients receiving either neuroleptic drug. The rating scales are not sensitive enough to monitor manic psychopathology; this accounts for the lack of statistically significant differences among drug groups at treatment termination, despite the widely disparate discharge rates.", "contents": "Psychoactive drugs in mania. A controlled comparison of lithium carbonate, chlorpromazine, and haloperidol. Lithium carbonate, haloperidol, and chlorpromazine hydrochloride were compared in a double-blind controlled study with severely ill hospitalized manics. Lithium carbonate and haloperidol produced a highly significant improvement of manic symptoms without sedation. Although producing considerable sedation, chlorpromazine did little to alter the underlying mania qualitatively. Qualitative differences between lithium carbonate and haloperidol indicate that, while haloperidol has a more dramatically rapid impact on behavior-motor activity, lithium carbonate acted more evenly on the entire manic picture, with total normalization realized during active treatment. The majority of lithium carbonate-treated patients met discharge criteria at study termination, but not the patients receiving either neuroleptic drug. The rating scales are not sensitive enough to monitor manic psychopathology; this accounts for the lack of statistically significant differences among drug groups at treatment termination, despite the widely disparate discharge rates."}
{"id": "PMID:1089402", "title": "Classification in schizophrenia.", "content": "Schizophrenia has been defined as an indentifiable disorder based on phenomenologic classification. Support for this concept is derived from consistent observations of a low frequency of the disorder in general populations throughout the world but substantially higher frequency of occurrence in siblings of affected individuals. The rates of concurrence in diagnosis for schizophrenia, surprisingly, vary in similar degree to those found for a series of physical disorders such as cervical cancer, emphysema and bronchitis, and electrocardiographic evaluations of cardiac disorder. The most recent findings from cross-national diagnostic studies of mental disorder uphold the need for a regular, more definitive classification system that is considered from both the service and research points of view.", "contents": "Classification in schizophrenia. Schizophrenia has been defined as an indentifiable disorder based on phenomenologic classification. Support for this concept is derived from consistent observations of a low frequency of the disorder in general populations throughout the world but substantially higher frequency of occurrence in siblings of affected individuals. The rates of concurrence in diagnosis for schizophrenia, surprisingly, vary in similar degree to those found for a series of physical disorders such as cervical cancer, emphysema and bronchitis, and electrocardiographic evaluations of cardiac disorder. The most recent findings from cross-national diagnostic studies of mental disorder uphold the need for a regular, more definitive classification system that is considered from both the service and research points of view."}
{"id": "PMID:1089403", "title": "Intravitreal injection of cephaloridine in the treatment of endophthalmitis.", "content": "Ocular toxic effects of intravitreal injection of cephaloridine in rabbits was evaluated, and none were produced in doses of 0.25 mg or less. Clinically, intravitreal injection of cephaloridine in doses of 5 mg or more produced small punctate hemorrhages in the vascularized portions of the rabbit retina within two days. With doses of 2.5 mg or less, no clinical changes were seen during the two weeks of observation. Histologically, one day following intravitreal injection of 0.5 to 10 mg of cephaloridine there was clumping of the outer segments of the photoreceptors and destruction of the retinal pigment epithelium. Ten milligrams of cephaloridine intravitreally injected caused definite electroretinogram (ERG) changes ten minutes after injection. After 24 hours, the ERG was extinguished. An experimentally induced Escherichia coli endophthalmitis was successfully treated after six hours by intravitreal injection of cephaloridine.", "contents": "Intravitreal injection of cephaloridine in the treatment of endophthalmitis. Ocular toxic effects of intravitreal injection of cephaloridine in rabbits was evaluated, and none were produced in doses of 0.25 mg or less. Clinically, intravitreal injection of cephaloridine in doses of 5 mg or more produced small punctate hemorrhages in the vascularized portions of the rabbit retina within two days. With doses of 2.5 mg or less, no clinical changes were seen during the two weeks of observation. Histologically, one day following intravitreal injection of 0.5 to 10 mg of cephaloridine there was clumping of the outer segments of the photoreceptors and destruction of the retinal pigment epithelium. Ten milligrams of cephaloridine intravitreally injected caused definite electroretinogram (ERG) changes ten minutes after injection. After 24 hours, the ERG was extinguished. An experimentally induced Escherichia coli endophthalmitis was successfully treated after six hours by intravitreal injection of cephaloridine."}
{"id": "PMID:1089426", "title": "Haemodynamic effects of the antiarrhythmic quaternary ammonium compound QX-572 in man.", "content": "The haemodynamic effects of N, N-bis(phenyl-carbamoylmethyl) dimethylammonium chloride (QX-572) in man were studied. A controlled study was performed to rule out a possible influence of the catheterization procedure as such on the results. Ten patients with mild to moderate aortic regurgitation were studied: based on clinical data the patients were divided into 2 groups of 5. Randomly it was decided that one group should constitute a control group receiving saline while the second group received QX-572 , MG/KG BODY WEIGHT. In both groups the administration was performed as a slow intravenous infusion during 30 minutes. Heart rate, pressures in brachial artery and right atrium, cardiac output, stroke volume, and systemic vascular resistance were determined before, during, and up to 30 minutes after completion of placebo or QX-572. These variable remained stable in the control group while QX-572 produced an increase in heart rate most pronounced at the end of the infusion period. A transient decrease in systolic and mean brachial artery pressure during the infusion, and during the same period a decrease in right atrial pressure. Cardiac output and systemic vascular resistance were unchanged by QX-572 but they were not measured during the infusion when the changes in pressures were most pronounced. QX-572 was thought to act as a peripheral vasodilator during the infusion. Left ventricular contractility was studied by means of pressure curves obtained from a catheter tip manometer placed in the left ventricle. The first derivative of the isovolumic left ventricular pressure at the highest level (45mmHg) common to all patients was used (dp/dt-45). No significant difference could be observed when comparing mean changes of dp/dt-45 for the two groups. In the control group there was a slight but significant increase in dp/dt-45 during the time of observation. In the QX-572 group the results varied between individuals. Two of the patients differed from all other patients in the control and QX-572 groups showing a decrease in dp/dt-45 which, when most pronounced at the end of the infusion period, was -31 and -28 per cent of the preinfusion levels, respectively. This decrease probably reflects reduction of contractility. It was concluded that QX-572 in a dose of 8 mg/kg body weight did not have any major haemodynamic drawbacks.", "contents": "Haemodynamic effects of the antiarrhythmic quaternary ammonium compound QX-572 in man. The haemodynamic effects of N, N-bis(phenyl-carbamoylmethyl) dimethylammonium chloride (QX-572) in man were studied. A controlled study was performed to rule out a possible influence of the catheterization procedure as such on the results. Ten patients with mild to moderate aortic regurgitation were studied: based on clinical data the patients were divided into 2 groups of 5. Randomly it was decided that one group should constitute a control group receiving saline while the second group received QX-572 , MG/KG BODY WEIGHT. In both groups the administration was performed as a slow intravenous infusion during 30 minutes. Heart rate, pressures in brachial artery and right atrium, cardiac output, stroke volume, and systemic vascular resistance were determined before, during, and up to 30 minutes after completion of placebo or QX-572. These variable remained stable in the control group while QX-572 produced an increase in heart rate most pronounced at the end of the infusion period. A transient decrease in systolic and mean brachial artery pressure during the infusion, and during the same period a decrease in right atrial pressure. Cardiac output and systemic vascular resistance were unchanged by QX-572 but they were not measured during the infusion when the changes in pressures were most pronounced. QX-572 was thought to act as a peripheral vasodilator during the infusion. Left ventricular contractility was studied by means of pressure curves obtained from a catheter tip manometer placed in the left ventricle. The first derivative of the isovolumic left ventricular pressure at the highest level (45mmHg) common to all patients was used (dp/dt-45). No significant difference could be observed when comparing mean changes of dp/dt-45 for the two groups. In the control group there was a slight but significant increase in dp/dt-45 during the time of observation. In the QX-572 group the results varied between individuals. Two of the patients differed from all other patients in the control and QX-572 groups showing a decrease in dp/dt-45 which, when most pronounced at the end of the infusion period, was -31 and -28 per cent of the preinfusion levels, respectively. This decrease probably reflects reduction of contractility. It was concluded that QX-572 in a dose of 8 mg/kg body weight did not have any major haemodynamic drawbacks."}
{"id": "PMID:1089427", "title": "Methylation of the ribosomal proteins in Escherichia coli. Nature and stoichiometry of the methylated amino acids in 50S ribosomal proteins.", "content": "Methylated ribosomal proteins from Escherichia coli 50S subunit are localized by growing cells in a medium containing (1-14C)methionine and (3H-methyl)-methionine and comparing the 3H/14C ratio for each of the 50S ribosomal proteins. The following proteins are methylated: L11, L1, L3, L5, L7, L8, L9, L12, L18, and L33. The nature and stoichiometry of the methylated amino acid(s) in each of the methylated proteins are determined. Protein L11 is the most heavily methylated of all the 50S subunit proteins. This protein has previously been implicated in the peptidyl transferase reaction during protein synthesis (K. H. Nierhaus and V. Montejo (1973), Proc. Nat. Acad. Sci. U. S. 47, 1588-1602). Three proteins (L1, L3, and L5) have intermediate levels of methylation and contain about 0.4-0.6 methyl groups each per molecule of protein. Five other proteins (L7, L8, L9, L12, and L18) are also methylated to a slight extent (-0.1 methyl group/molecule of protein). One unknown methylated neutral amino acid was detected in protein L11 and at least one and possibly two other unidentified methylated amino acids appeared to be present in protein L33.", "contents": "Methylation of the ribosomal proteins in Escherichia coli. Nature and stoichiometry of the methylated amino acids in 50S ribosomal proteins. Methylated ribosomal proteins from Escherichia coli 50S subunit are localized by growing cells in a medium containing (1-14C)methionine and (3H-methyl)-methionine and comparing the 3H/14C ratio for each of the 50S ribosomal proteins. The following proteins are methylated: L11, L1, L3, L5, L7, L8, L9, L12, L18, and L33. The nature and stoichiometry of the methylated amino acid(s) in each of the methylated proteins are determined. Protein L11 is the most heavily methylated of all the 50S subunit proteins. This protein has previously been implicated in the peptidyl transferase reaction during protein synthesis (K. H. Nierhaus and V. Montejo (1973), Proc. Nat. Acad. Sci. U. S. 47, 1588-1602). Three proteins (L1, L3, and L5) have intermediate levels of methylation and contain about 0.4-0.6 methyl groups each per molecule of protein. Five other proteins (L7, L8, L9, L12, and L18) are also methylated to a slight extent (-0.1 methyl group/molecule of protein). One unknown methylated neutral amino acid was detected in protein L11 and at least one and possibly two other unidentified methylated amino acids appeared to be present in protein L33."}
{"id": "PMID:1089428", "title": "Apparent high degree of asymmetry of protein arrangement in the Escherichia coli outer cell envelope membrane.", "content": "Ghosts from Escherichia coli have been oxidized with CuSO4-o-phenanthroline or ferricyanide-ferrocene. Upon oxidation they became resistant to boiling dodecyl sulfate. The resulting rod-shaped \"oxidation containers\" apparently held together by disulfide bridges, are practically pure protein. They are soluble in dodecyl sulfate when reduced and they contain a set of about 30 different polypeptide chains. The four major ghost membrane proteins are not represented among the \"oxidation proteins.\" Comparison of data obtained from digestion of ghosts with trypsin or particle-bound trypsin showed that most of the \"oxidation proteins\" appear to be located at the outer surface of the ghost membrane which is derived from the outer cell envelope membrane. One of the major ghost membrane proteins, II, is partially digested by trypsin, and it is shown that its trypsin sensitive part is also exposed only at the outer surface of the ghost membrane. Native cells could be oxidized only with low yields of \"oxidation containers.\" However, cell envelopes prepared without detergents or chelating agents, as well as cells depleted of phospholipid or treated with sucrose-Triton X-100, are completely accessible to oxidation. In each case, the same set of proteins as that present in \"oxidation containers\" from ghosts was found to be covalently linked. Treatment of cells with trypsin caused the loss of about five \"oxidation proteins\" and a complete loss of oxidizability of the ghosts derived from these cells. It therefore appears that arrangement and localization of the \"oxidation proteins\" are not greatly different in cells and in ghosts, i.e., that these proteins are also situated asymmetrically at the outer cell envelope membrane.", "contents": "Apparent high degree of asymmetry of protein arrangement in the Escherichia coli outer cell envelope membrane. Ghosts from Escherichia coli have been oxidized with CuSO4-o-phenanthroline or ferricyanide-ferrocene. Upon oxidation they became resistant to boiling dodecyl sulfate. The resulting rod-shaped \"oxidation containers\" apparently held together by disulfide bridges, are practically pure protein. They are soluble in dodecyl sulfate when reduced and they contain a set of about 30 different polypeptide chains. The four major ghost membrane proteins are not represented among the \"oxidation proteins.\" Comparison of data obtained from digestion of ghosts with trypsin or particle-bound trypsin showed that most of the \"oxidation proteins\" appear to be located at the outer surface of the ghost membrane which is derived from the outer cell envelope membrane. One of the major ghost membrane proteins, II, is partially digested by trypsin, and it is shown that its trypsin sensitive part is also exposed only at the outer surface of the ghost membrane. Native cells could be oxidized only with low yields of \"oxidation containers.\" However, cell envelopes prepared without detergents or chelating agents, as well as cells depleted of phospholipid or treated with sucrose-Triton X-100, are completely accessible to oxidation. In each case, the same set of proteins as that present in \"oxidation containers\" from ghosts was found to be covalently linked. Treatment of cells with trypsin caused the loss of about five \"oxidation proteins\" and a complete loss of oxidizability of the ghosts derived from these cells. It therefore appears that arrangement and localization of the \"oxidation proteins\" are not greatly different in cells and in ghosts, i.e., that these proteins are also situated asymmetrically at the outer cell envelope membrane."}
{"id": "PMID:1089429", "title": "Recognition of the 3' terminus of 2'-O-aminoacyl transfer ribonucleic acid by the acceptor site of ribosomal peptidyltransferase.", "content": "The interaction of the 3' terminus of 2'- and 3'-O-aminoacyl-tRNA with the peptidyltransferase A site of Escherichia coli ribosomes has been studied using the following aminoacyl oligonucleotides as models of the 3' terminus of AA-tRNA: C-A-Phe, C-A(2'Phe)H, C-A(2'H)Phe, C-A(2'Phe)Me, C-A(2'Me)Phe, C-A(2'Gly)H, and C-A(2'H)Gly. The transfer of Ac-(14C)Phe from the Ac-(14C)Phe-tRNA-oly(U)-70S ribosome complex to puromycin (10-4 and 10-5 M) was inhibited by C-A-Phe, C-A(2'Phe)H, C-A(2'H)Phe, C-A(2'Gly)H, and C-A(2'H)Gly. Kinetic analysis of the inhibition of Ac(14C)Phe-puromycin formation by C-A(2'Phe)H failed to show simple competitive inhibition. Binding of C-A-C-C-A-(14C)Phe to 70S ribosomes in the presence of an excess of deacylated tRNA was also inhibited by C-A-Phe, C-A(2'Phe)H, C-A(2'H)Phe, C-A(2'Phe)Me, and C-A(2'Me)Phe. It appears that the acceptor site of peptidyltransferase can recognize the 3' terminus of either 2'- or 3'-O-AA-tRNA, with preference for the 2' isomer. It therefore follows that 2'-O-AA-tRNA amy be bound to ribosomes prior to peptide bone formation and that 3'-O-AA-tRNA, which is used exclusively by peptidyltransferase as an acceptor, is supplied by 2' leads to 3' transacylation occuring at the peptidyltransferase A site.", "contents": "Recognition of the 3' terminus of 2'-O-aminoacyl transfer ribonucleic acid by the acceptor site of ribosomal peptidyltransferase. The interaction of the 3' terminus of 2'- and 3'-O-aminoacyl-tRNA with the peptidyltransferase A site of Escherichia coli ribosomes has been studied using the following aminoacyl oligonucleotides as models of the 3' terminus of AA-tRNA: C-A-Phe, C-A(2'Phe)H, C-A(2'H)Phe, C-A(2'Phe)Me, C-A(2'Me)Phe, C-A(2'Gly)H, and C-A(2'H)Gly. The transfer of Ac-(14C)Phe from the Ac-(14C)Phe-tRNA-oly(U)-70S ribosome complex to puromycin (10-4 and 10-5 M) was inhibited by C-A-Phe, C-A(2'Phe)H, C-A(2'H)Phe, C-A(2'Gly)H, and C-A(2'H)Gly. Kinetic analysis of the inhibition of Ac(14C)Phe-puromycin formation by C-A(2'Phe)H failed to show simple competitive inhibition. Binding of C-A-C-C-A-(14C)Phe to 70S ribosomes in the presence of an excess of deacylated tRNA was also inhibited by C-A-Phe, C-A(2'Phe)H, C-A(2'H)Phe, C-A(2'Phe)Me, and C-A(2'Me)Phe. It appears that the acceptor site of peptidyltransferase can recognize the 3' terminus of either 2'- or 3'-O-AA-tRNA, with preference for the 2' isomer. It therefore follows that 2'-O-AA-tRNA amy be bound to ribosomes prior to peptide bone formation and that 3'-O-AA-tRNA, which is used exclusively by peptidyltransferase as an acceptor, is supplied by 2' leads to 3' transacylation occuring at the peptidyltransferase A site."}
{"id": "PMID:1089430", "title": "Multiple forms of phosphodeoxyribomutase from Escherichia coli. Physical and chemical characterization.", "content": "Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different molecular forms of the enzyme thus isolated were shown not to be products of the column chromatography per se. The amino acid composition as well as the N-terminal amino acid were found to be identical for the different forms. Molecular weight determinations demonstrated that all four forms of the enzyme consist of a single polypeptide chain with a molecular weight of 45,000 plus or minus 1000. Measurements of partial specific volumes, sedimentation coefficients, and absorption coefficients for form I and form II did not reveal any differences. It is concluded that the multiple forms of phosphodeoxyribomutase are caused by modifications of the polypeptide chain. Evidence is presented that form II is formed in vitro from form I by deamidation. It is probable that this deamidation occurs in vivo also. The different forms displayed only minor changes with respect to KM for substrate and cofactor. Greater differences seem to exist among the four enzyme forms with respect to VM and to cobalt binding.", "contents": "Multiple forms of phosphodeoxyribomutase from Escherichia coli. Physical and chemical characterization. Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different molecular forms of the enzyme thus isolated were shown not to be products of the column chromatography per se. The amino acid composition as well as the N-terminal amino acid were found to be identical for the different forms. Molecular weight determinations demonstrated that all four forms of the enzyme consist of a single polypeptide chain with a molecular weight of 45,000 plus or minus 1000. Measurements of partial specific volumes, sedimentation coefficients, and absorption coefficients for form I and form II did not reveal any differences. It is concluded that the multiple forms of phosphodeoxyribomutase are caused by modifications of the polypeptide chain. Evidence is presented that form II is formed in vitro from form I by deamidation. It is probable that this deamidation occurs in vivo also. The different forms displayed only minor changes with respect to KM for substrate and cofactor. Greater differences seem to exist among the four enzyme forms with respect to VM and to cobalt binding."}
{"id": "PMID:1089431", "title": "Oxidation of methionine residues of porcine and bovine pepsins.", "content": "By treating porcine and bovine pepsins with H2O2 at pH 3.2, 3.5 of the 4 methionine residues of porcine pepsin and 1.6 of the 3 residues of bovine pepsin were oxidized to methionine sulfoxide. The effect of modification on activity varied with the substrate. There were no significant changes in catalytic constants in the hydrolysis of acetyl-L-phenylalanyl-L-tyrosine by both pepsins and in the hydrolysis of benzyloxycarbonyl-L-glutamyl-L-tyrosine by porcine pepsin. Hydrolysis of benzyloxycarbonyl-L-glutamyl-L-tyrosine by bovine pepsin was too slow to measure. With benzyloxycarbonyl-L-histidyl-L-phenylalanyl-L-tryptophan ethyl ester as substrate, the modification decreased the catalytic efficiency (kcat/Km) by two-thirds for porcine pepsin and by half for bovine pepsin. With hemoglobin substrate, digestion was significantly less than with native pepsin for modified porcine pepsin, and slightly less for modified bovine pepsin. The results are interpreted as indicating the presence of a methionine residue that participates in the binding of long substrates, but is not close enough to the active site to reach short substrates. Cleavage of the modified pepsins with cyanogen bromide identified the methionine nearest the carboxyl terminus of both pepsins as a resiude that remained partially unmodified.", "contents": "Oxidation of methionine residues of porcine and bovine pepsins. By treating porcine and bovine pepsins with H2O2 at pH 3.2, 3.5 of the 4 methionine residues of porcine pepsin and 1.6 of the 3 residues of bovine pepsin were oxidized to methionine sulfoxide. The effect of modification on activity varied with the substrate. There were no significant changes in catalytic constants in the hydrolysis of acetyl-L-phenylalanyl-L-tyrosine by both pepsins and in the hydrolysis of benzyloxycarbonyl-L-glutamyl-L-tyrosine by porcine pepsin. Hydrolysis of benzyloxycarbonyl-L-glutamyl-L-tyrosine by bovine pepsin was too slow to measure. With benzyloxycarbonyl-L-histidyl-L-phenylalanyl-L-tryptophan ethyl ester as substrate, the modification decreased the catalytic efficiency (kcat/Km) by two-thirds for porcine pepsin and by half for bovine pepsin. With hemoglobin substrate, digestion was significantly less than with native pepsin for modified porcine pepsin, and slightly less for modified bovine pepsin. The results are interpreted as indicating the presence of a methionine residue that participates in the binding of long substrates, but is not close enough to the active site to reach short substrates. Cleavage of the modified pepsins with cyanogen bromide identified the methionine nearest the carboxyl terminus of both pepsins as a resiude that remained partially unmodified."}
{"id": "PMID:1089432", "title": "Isolation and characterization of two outer membrane preparations from Escherichia coli.", "content": "A method was developed for releasing specifically a part of outer membrane during spheroplast formation. A highly purified outer membrane (outer membrane I) was obtained from the spheroplast medium by isopycnic sucrose gradient centrifugation. The remaining outer membrane (outer membrane II) and cytoplasmic membrane was also isolated from the spheroplasts by the isopycnic centrifugation. Two outer membrane preparations were different from the cytoplasmic membrane in protein composition, enzyme localization, phospholipid composition, lipopolysaccharide content and electron micrographs. Although outer membranes I and II were almost the same in various respects, they seemed to be different from each other under electron microscope and in cardiolipin content. It is suggested that the outer membrane I and the outer membrane II, at least a part of the outer membrane II, are integrated in a different fashion in the outer-most layer of Escherichia coli cell surface.", "contents": "Isolation and characterization of two outer membrane preparations from Escherichia coli. A method was developed for releasing specifically a part of outer membrane during spheroplast formation. A highly purified outer membrane (outer membrane I) was obtained from the spheroplast medium by isopycnic sucrose gradient centrifugation. The remaining outer membrane (outer membrane II) and cytoplasmic membrane was also isolated from the spheroplasts by the isopycnic centrifugation. Two outer membrane preparations were different from the cytoplasmic membrane in protein composition, enzyme localization, phospholipid composition, lipopolysaccharide content and electron micrographs. Although outer membranes I and II were almost the same in various respects, they seemed to be different from each other under electron microscope and in cardiolipin content. It is suggested that the outer membrane I and the outer membrane II, at least a part of the outer membrane II, are integrated in a different fashion in the outer-most layer of Escherichia coli cell surface."}
{"id": "PMID:1089433", "title": "Extracellular bicarbonate ions and insulin secretion.", "content": "The role of bicarbonate ions in insulin release was studied with incubated and perifused isolated rat islets of Langerhans. In the absence of NaHC03, the early phase of glucose-induced secretion was completely abolished and the second phase inhibited by approximately 65%. The insulinotropic effect of the sugar was totally restored after reintroduction of the ion in the medium. The monophasic secretory after reintroduction of the ion in the medium. The monophasic secretory response to tolbutamide was also markedly diminished by omission of NaHC03, WHereas the release evoked by a high concentration of K+ was very little affected. CO2 wwas unable to substitute for HC03minus, but small concentrations of the anion (3to 5mM) WEre sufficient to ensure a normal response to glucose.", "contents": "Extracellular bicarbonate ions and insulin secretion. The role of bicarbonate ions in insulin release was studied with incubated and perifused isolated rat islets of Langerhans. In the absence of NaHC03, the early phase of glucose-induced secretion was completely abolished and the second phase inhibited by approximately 65%. The insulinotropic effect of the sugar was totally restored after reintroduction of the ion in the medium. The monophasic secretory after reintroduction of the ion in the medium. The monophasic secretory response to tolbutamide was also markedly diminished by omission of NaHC03, WHereas the release evoked by a high concentration of K+ was very little affected. CO2 wwas unable to substitute for HC03minus, but small concentrations of the anion (3to 5mM) WEre sufficient to ensure a normal response to glucose."}
{"id": "PMID:1089435", "title": "[Focal myofibrillar disorganization in acute metabolic impairment of somatic musculature].", "content": "Skeletal muscles of rats and rabbits were studied by the target electron microscoptic and polarization-optic methods after administration of toxic substances and experimental acute ischemia of the extremities. Two types of foci of the myofibrillae disaggregation were described along with contracture injuries. The first occurred on the basis of relaxation and was expressed in total dysaggregation and lysis of myofibrillae; with the second type lysis occurred in the partially contracted fibers and began in the I-discs leading to diastasis of A-discs. The first type of the foci is characteristic chiefly of the white fibers, and the second one--of the red.", "contents": "[Focal myofibrillar disorganization in acute metabolic impairment of somatic musculature]. Skeletal muscles of rats and rabbits were studied by the target electron microscoptic and polarization-optic methods after administration of toxic substances and experimental acute ischemia of the extremities. Two types of foci of the myofibrillae disaggregation were described along with contracture injuries. The first occurred on the basis of relaxation and was expressed in total dysaggregation and lysis of myofibrillae; with the second type lysis occurred in the partially contracted fibers and began in the I-discs leading to diastasis of A-discs. The first type of the foci is characteristic chiefly of the white fibers, and the second one--of the red."}
{"id": "PMID:1089436", "title": "[Studies on the Concanavalin A-receptors of rabbit erythrocytes and of erythyrocyte shadows using immunoelectron microscopy].", "content": "The authors report on the immuno electron microscopic demonstration of Concanavalin A-receptors on the erythrocyte membrane. Immuno freeze-etching studies evidenced a focal arrangement of Concanavalin A-receptors on intact erythrocytes as well as on erythrocyte ghosts. Obviously hemolysis by distilled water does not produce changes in the pattern of distribution of Concanavalin A-receptors whereas slight aggregation of plasma membrane intramembraneous particles occured. No unambigous spatial relationship between Concanavalin A-receptors and the plasma membrane intramembraneous particles (intrinsic proteins) of erythrocytes could be observed.", "contents": "[Studies on the Concanavalin A-receptors of rabbit erythrocytes and of erythyrocyte shadows using immunoelectron microscopy]. The authors report on the immuno electron microscopic demonstration of Concanavalin A-receptors on the erythrocyte membrane. Immuno freeze-etching studies evidenced a focal arrangement of Concanavalin A-receptors on intact erythrocytes as well as on erythrocyte ghosts. Obviously hemolysis by distilled water does not produce changes in the pattern of distribution of Concanavalin A-receptors whereas slight aggregation of plasma membrane intramembraneous particles occured. No unambigous spatial relationship between Concanavalin A-receptors and the plasma membrane intramembraneous particles (intrinsic proteins) of erythrocytes could be observed."}
{"id": "PMID:1089437", "title": "The continent ileostomy of Kock (ileal intra-abdominal reservoir). Experience with 9 cases operated on for ulcerative colitis.", "content": "A series of 9 patients with ulcerative colitis undergoing proctocolectomy and ileal intra-adbominal reservoir is reported. Eight patients are continent and 1 is incontinent. Early and late postoperative complications, work capacity and social and sexual functions are described.", "contents": "The continent ileostomy of Kock (ileal intra-abdominal reservoir). Experience with 9 cases operated on for ulcerative colitis. A series of 9 patients with ulcerative colitis undergoing proctocolectomy and ileal intra-adbominal reservoir is reported. Eight patients are continent and 1 is incontinent. Early and late postoperative complications, work capacity and social and sexual functions are described."}
{"id": "PMID:1089434", "title": "[X-ray diffraction studies of muscles].", "content": "A brief account of the modern state of X-ray diffraction studies of muscles is presented. Recently many investigaters payed particular attention to concrete changes taking place in structural-executive (protofibrillar) apparatus of muscles at the change of their state. The drawback of these works is the necessity of interpreting the data on the basis of model ideas having definite variability. Our studies here presented differe by the approach to the problem and to a certain extent by the methods applied. Our main task was to study the dynamics of structural changes in muscles while the latter perform their working function. Special attention was payed to the studies of muscles at different shortening eithor on living or fixed samples. The effect of some conventional fixators on structural parameters evaluated by X-ray analysis was studied for the first time. A means of stroboscopic accumulation of X-ray diffraction patterns for periodic fast processes was developed. One of our main tasks was to find the range of applicability sliding mechanism hypothesis for describing muscle shortening. It was observed that the sliding mechanism held true for the desscription of the structure of muscles shortened in a physiological range. X-ray diffraction patterns were obtained which were different for the shortenings according to the sliding mechanism and those with A-disc shortening and during supercontraction. The limits of the sliding model were evaluated by some of these characteristics for muscle of different composition and properties. The problem of the forces realizing muscle shortening is discussed, some of the author's studies being taken into account. Muscle should be assessed as a complex heteromorph biological object and as a complex liquid crystalline system to create the models of its molecular structure and to elucidate the precise mechanism of its functional activity.", "contents": "[X-ray diffraction studies of muscles]. A brief account of the modern state of X-ray diffraction studies of muscles is presented. Recently many investigaters payed particular attention to concrete changes taking place in structural-executive (protofibrillar) apparatus of muscles at the change of their state. The drawback of these works is the necessity of interpreting the data on the basis of model ideas having definite variability. Our studies here presented differe by the approach to the problem and to a certain extent by the methods applied. Our main task was to study the dynamics of structural changes in muscles while the latter perform their working function. Special attention was payed to the studies of muscles at different shortening eithor on living or fixed samples. The effect of some conventional fixators on structural parameters evaluated by X-ray analysis was studied for the first time. A means of stroboscopic accumulation of X-ray diffraction patterns for periodic fast processes was developed. One of our main tasks was to find the range of applicability sliding mechanism hypothesis for describing muscle shortening. It was observed that the sliding mechanism held true for the desscription of the structure of muscles shortened in a physiological range. X-ray diffraction patterns were obtained which were different for the shortenings according to the sliding mechanism and those with A-disc shortening and during supercontraction. The limits of the sliding model were evaluated by some of these characteristics for muscle of different composition and properties. The problem of the forces realizing muscle shortening is discussed, some of the author's studies being taken into account. Muscle should be assessed as a complex heteromorph biological object and as a complex liquid crystalline system to create the models of its molecular structure and to elucidate the precise mechanism of its functional activity."}
{"id": "PMID:1089438", "title": "Phenol sympathectomy in the treatment of intermittent claudication: a controlled clinical trail.", "content": "Twenty-five patients with intermittent claudication were randomly allocated to treatment either by injection of phenol into the lumbar sympathetic chain or by injection of local anaesthetic subcutaneously. On the day after the injection, sympathectomy, assessed by skin temperature change, was achieved in 85 per cent of the phenol group. At 1 month subjective improvement was claimed by 45 per cent of the patients in the sympathectomized group and by 64 per cent in the control group; at 3 months the figures were 25 and 45 per cent respectively. Treadmill testing at two exercise loads showed no significant difference in claudication distance or stopping time between the two groups. Calf blood pressure response following exercise was not affected by sympathectomy. There is no subjective or objective evidence that phenol sympathectomy improves intermittent claudication.", "contents": "Phenol sympathectomy in the treatment of intermittent claudication: a controlled clinical trail. Twenty-five patients with intermittent claudication were randomly allocated to treatment either by injection of phenol into the lumbar sympathetic chain or by injection of local anaesthetic subcutaneously. On the day after the injection, sympathectomy, assessed by skin temperature change, was achieved in 85 per cent of the phenol group. At 1 month subjective improvement was claimed by 45 per cent of the patients in the sympathectomized group and by 64 per cent in the control group; at 3 months the figures were 25 and 45 per cent respectively. Treadmill testing at two exercise loads showed no significant difference in claudication distance or stopping time between the two groups. Calf blood pressure response following exercise was not affected by sympathectomy. There is no subjective or objective evidence that phenol sympathectomy improves intermittent claudication."}
{"id": "PMID:1089439", "title": "A clinical trial designed to discover if the primary treatment of varicose veins should be by Fegan's method or by an operation.", "content": "1. The current practice of operating upon patients with varicose veins as the treatment of choice costs more than xi15 million a year. 2. The bulk of this expense is due to the length of time that patients are kept in the ward. If they were treated as outpatients, either by Fegan's method or by operation, the cost would be reduced to one-tenth of what is now spent. Even if the operation were done on a \"short-stay\" basis the cost would be reduced to one-third or less. 3. Although the long term results of Fegan's method are uncertain there is no doubt that it gives better immediate results than an operation. Moreover, there is no particular type of varicosity of shape of limb which precludes its use.", "contents": "A clinical trial designed to discover if the primary treatment of varicose veins should be by Fegan's method or by an operation. 1. The current practice of operating upon patients with varicose veins as the treatment of choice costs more than xi15 million a year. 2. The bulk of this expense is due to the length of time that patients are kept in the ward. If they were treated as outpatients, either by Fegan's method or by operation, the cost would be reduced to one-tenth of what is now spent. Even if the operation were done on a \"short-stay\" basis the cost would be reduced to one-third or less. 3. Although the long term results of Fegan's method are uncertain there is no doubt that it gives better immediate results than an operation. Moreover, there is no particular type of varicosity of shape of limb which precludes its use."}
{"id": "PMID:1089440", "title": "Combined immunodeficiency disease associated with adenosine deaminase deficiency. Report on a workshop held in Albany, New York, October 1, 1973.", "content": "Fifty-five children with CID and known ADA status were studies at a workshop held in Albany, New York. Erythrocyte ADA determinations were performed in 22 of the 55 patients, 13 of whom were ADA negative. The ADA defect appears to be transmitted as an autosomal recessive trait. Some patients with CID and ADA deficiency have characteristic radiologic abnormalities of the skeleton, which are not found in other illnesses. The thymus glands of all patients with CID and ADA deficiency who could be examined have evidence of thymic involution manifested by presence of Hassall's corpuscles and differentiated germinal epithelium; this is in contrast to \"classic\" thymus findings in CID with normal ADA. Adenosine deaminase probably plays an important, although as yet undefined, role in lymphocyte development and/or function. The deficiency of ADA in CID is the first enzyme defect observed in a deficiency disease of specific immunity.", "contents": "Combined immunodeficiency disease associated with adenosine deaminase deficiency. Report on a workshop held in Albany, New York, October 1, 1973. Fifty-five children with CID and known ADA status were studies at a workshop held in Albany, New York. Erythrocyte ADA determinations were performed in 22 of the 55 patients, 13 of whom were ADA negative. The ADA defect appears to be transmitted as an autosomal recessive trait. Some patients with CID and ADA deficiency have characteristic radiologic abnormalities of the skeleton, which are not found in other illnesses. The thymus glands of all patients with CID and ADA deficiency who could be examined have evidence of thymic involution manifested by presence of Hassall's corpuscles and differentiated germinal epithelium; this is in contrast to \"classic\" thymus findings in CID with normal ADA. Adenosine deaminase probably plays an important, although as yet undefined, role in lymphocyte development and/or function. The deficiency of ADA in CID is the first enzyme defect observed in a deficiency disease of specific immunity."}
{"id": "PMID:1089442", "title": "Spina bifida children attending ordinary schools.", "content": "From September 1971 to September 1973 a policy was actively followed in South Gloucestershire of placing spina bifida children in ordinary schools wherever possible. This was achieved successfully in 14 cases out of a possible 24. Special attention was paid to the selection of children and schools. The main contraindications to attendance at ordinary school were: below average intelligence, special perceptual problems, the need for intensive physiotherapy, and problem incontinence-particularly infaecal continence. Success often depended on special provisions-for example, transport, aids to mobility, peripatetic physiotherapists. Personal attendants (welfare assistants) could be allocated to each of the more severely handicapped children. The lack of facilities for handicapped pupils in comprehensive schools is a matter needing urgent review.", "contents": "Spina bifida children attending ordinary schools. From September 1971 to September 1973 a policy was actively followed in South Gloucestershire of placing spina bifida children in ordinary schools wherever possible. This was achieved successfully in 14 cases out of a possible 24. Special attention was paid to the selection of children and schools. The main contraindications to attendance at ordinary school were: below average intelligence, special perceptual problems, the need for intensive physiotherapy, and problem incontinence-particularly infaecal continence. Success often depended on special provisions-for example, transport, aids to mobility, peripatetic physiotherapists. Personal attendants (welfare assistants) could be allocated to each of the more severely handicapped children. The lack of facilities for handicapped pupils in comprehensive schools is a matter needing urgent review."}
{"id": "PMID:1089443", "title": "Renal excretion of fluoride in renal failure and after renal transplantation.", "content": "We have compared the renal excretion of fluoride in a variety of patients with chronic renal failure maintained with and without protein restriction before and during regular dialysis treatment and after transplantation. The patients tended to continue to excrete normal dietary loads of fluoride quite well until renal function was seriously reduced. From a regression of function on excretion the mean level of creatinine clearance when a normal dietary load of fluoride 0.0526 plus or minus 0.019 mmol/2 h (1.0 plus or minus 0.36 mg/24h) has a 90% chance of being excreted lies around 16 ml/min, a level when most patients with renal failure will be symptomatic. Acute loading of such patients with additional fluoride in the form of sodium fluoride from 40 mg to 60 mg/day showed a twofold to threefold increase of serum fluoride concentrations, slight increases in urinary fluoride excretion, and heavy tissue absorption, suggesting that prior fluoride loading of the skeleton had not taken place. These effects contrasted with those in one patient with normal renal function and with those in one patient with skeletal saturation due to prolonged loading. After renal transplantation fluoride excretion increased but reached normal levels within three months of satisfactory function, suggesting that fluoride loading in renal failure and during regular dialysis therapy had not been excessive.", "contents": "Renal excretion of fluoride in renal failure and after renal transplantation. We have compared the renal excretion of fluoride in a variety of patients with chronic renal failure maintained with and without protein restriction before and during regular dialysis treatment and after transplantation. The patients tended to continue to excrete normal dietary loads of fluoride quite well until renal function was seriously reduced. From a regression of function on excretion the mean level of creatinine clearance when a normal dietary load of fluoride 0.0526 plus or minus 0.019 mmol/2 h (1.0 plus or minus 0.36 mg/24h) has a 90% chance of being excreted lies around 16 ml/min, a level when most patients with renal failure will be symptomatic. Acute loading of such patients with additional fluoride in the form of sodium fluoride from 40 mg to 60 mg/day showed a twofold to threefold increase of serum fluoride concentrations, slight increases in urinary fluoride excretion, and heavy tissue absorption, suggesting that prior fluoride loading of the skeleton had not taken place. These effects contrasted with those in one patient with normal renal function and with those in one patient with skeletal saturation due to prolonged loading. After renal transplantation fluoride excretion increased but reached normal levels within three months of satisfactory function, suggesting that fluoride loading in renal failure and during regular dialysis therapy had not been excessive."}
{"id": "PMID:1089445", "title": "Pituitary sensitivity to 50 micro g LH/FSH-RH in subjects with anorexia nervosa in acute and recovery stages.", "content": "Gonadotrophic output was measured in response to a 50-mug dose of luteinizing hormone/follicle stimulating hormone-releasing hormone (LH/FSH-RH) in subjects with anorexia nervosa. A diminished response was found in subjects of low body weight, and in some a response occurred with increments of plasma FSH greater than those of LH. With restoration of body weight the response to the releasing factor was similar to that usually observed in healthy men and women.", "contents": "Pituitary sensitivity to 50 micro g LH/FSH-RH in subjects with anorexia nervosa in acute and recovery stages. Gonadotrophic output was measured in response to a 50-mug dose of luteinizing hormone/follicle stimulating hormone-releasing hormone (LH/FSH-RH) in subjects with anorexia nervosa. A diminished response was found in subjects of low body weight, and in some a response occurred with increments of plasma FSH greater than those of LH. With restoration of body weight the response to the releasing factor was similar to that usually observed in healthy men and women."}
{"id": "PMID:1089446", "title": "Effect of different doses of chlorthalidone on blood pressure, serum potassium, and serum urate.", "content": "Chlorthalidone given to 40 hypertensive women significantly decreased blood pressure and serum potassium levels and increased the serum urate concentration.  There were no individual correlations between the reduction in blood pressure and the decrease in serum potassium or the increase in serum urate.  A reduction in dosage from 50 mg daily to 50 mg three times a week produced no significant changes in the diastolic or mean blood pressures though the systolic blood pressure was moderately increased.  Concomitantly, serum potassium increased and serum urate decreased significantly on the lower chlorthalidone dose.  We conclude that high doses of oral diuretics compared with lower ones are of limited further benefit and may increase the risk of clinically significant hypokalaemia and hyperuricaemia.", "contents": "Effect of different doses of chlorthalidone on blood pressure, serum potassium, and serum urate. Chlorthalidone given to 40 hypertensive women significantly decreased blood pressure and serum potassium levels and increased the serum urate concentration.  There were no individual correlations between the reduction in blood pressure and the decrease in serum potassium or the increase in serum urate.  A reduction in dosage from 50 mg daily to 50 mg three times a week produced no significant changes in the diastolic or mean blood pressures though the systolic blood pressure was moderately increased.  Concomitantly, serum potassium increased and serum urate decreased significantly on the lower chlorthalidone dose.  We conclude that high doses of oral diuretics compared with lower ones are of limited further benefit and may increase the risk of clinically significant hypokalaemia and hyperuricaemia."}
{"id": "PMID:1089455", "title": "Brain stem reticular units: some properties of the course and origin of the ascending trajectory.", "content": "Experiments were conducted in acutely prepared cats anesthetized with halothane-nitrous oxide. Single units were recorded in the mesencephalic and rostral rhombencephalic reticular core, and their ascending axons were stimulated in the mesencephalon, diencephalon, and telencephalon. The locations of stimulation and recording sites were determined by histological examination of the brains. Antidromic spikes were elicited by electrodes with a stimulus spread of approximately 300 mum and were collided with spontaneous (orthodromic) spikes to confirm antidromicity. Projecting axons were found to be scattered diffusely in the central mesencephalon in both the tegmentum and tectum. At the mesodiencephalic junction, the dorsal extent of the pathway lay in the posterior commissure and the ventral extent in the substantia nigra. More rostrally, a ventral group of axons was found in or near the supraoptic nucleus in the hypothalamus, and near the floor of the forebrain in the basal telencephalon. In agreement with previous reports, a dorsal component was located in the medial thalamus, most often in the nucleus centralis lateralis. The conduction velocity of each axon was determined, and they suggest a wide range of axon size in the ascending pathways. There may be a slight predominance of smaller fibers in the dorsal component and of larger fibers in the ventral component; however, all parts of the pathways were relatively heterogenous. The units from which projecting axons arose were found in the rostral rhombencephalon, and no units sending their axons along the restricted pathways investigated were identified rostral or caudal to this region. However, no conclusion is drawn concerning units within the region extending from 0.0 to 0.6 mm of the midline or other brain stem regions known to be monoamine-rich and to have ascending axons; these cellular populations were not examined.", "contents": "Brain stem reticular units: some properties of the course and origin of the ascending trajectory. Experiments were conducted in acutely prepared cats anesthetized with halothane-nitrous oxide. Single units were recorded in the mesencephalic and rostral rhombencephalic reticular core, and their ascending axons were stimulated in the mesencephalon, diencephalon, and telencephalon. The locations of stimulation and recording sites were determined by histological examination of the brains. Antidromic spikes were elicited by electrodes with a stimulus spread of approximately 300 mum and were collided with spontaneous (orthodromic) spikes to confirm antidromicity. Projecting axons were found to be scattered diffusely in the central mesencephalon in both the tegmentum and tectum. At the mesodiencephalic junction, the dorsal extent of the pathway lay in the posterior commissure and the ventral extent in the substantia nigra. More rostrally, a ventral group of axons was found in or near the supraoptic nucleus in the hypothalamus, and near the floor of the forebrain in the basal telencephalon. In agreement with previous reports, a dorsal component was located in the medial thalamus, most often in the nucleus centralis lateralis. The conduction velocity of each axon was determined, and they suggest a wide range of axon size in the ascending pathways. There may be a slight predominance of smaller fibers in the dorsal component and of larger fibers in the ventral component; however, all parts of the pathways were relatively heterogenous. The units from which projecting axons arose were found in the rostral rhombencephalon, and no units sending their axons along the restricted pathways investigated were identified rostral or caudal to this region. However, no conclusion is drawn concerning units within the region extending from 0.0 to 0.6 mm of the midline or other brain stem regions known to be monoamine-rich and to have ascending axons; these cellular populations were not examined."}
{"id": "PMID:1089458", "title": "Rostral projections from the hypothalamic arcuate nucleus.", "content": "The response of neurones in the medial basal hypothalamus to electrical stimulation in the suprachiasmatic/preoptic region has been studied in rats anaesthetised with urethane. Neurones in the hypothalamic arcuate, periventricular, ventromedial and dorsal premammillary nuclei were antidromically activated by the stimulation showing that such cells send processes in rostral direction outside the median eminence. Tuberoinfundibular and rostrally projecting units were found interspersed in the \"hypophysiotrophic\" area.", "contents": "Rostral projections from the hypothalamic arcuate nucleus. The response of neurones in the medial basal hypothalamus to electrical stimulation in the suprachiasmatic/preoptic region has been studied in rats anaesthetised with urethane. Neurones in the hypothalamic arcuate, periventricular, ventromedial and dorsal premammillary nuclei were antidromically activated by the stimulation showing that such cells send processes in rostral direction outside the median eminence. Tuberoinfundibular and rostrally projecting units were found interspersed in the \"hypophysiotrophic\" area."}
{"id": "PMID:1089460", "title": "The fine structural localization of glutamate decarboxylase in developing axonal processes and presynaptic terminals of rodent cerebellum.", "content": "The immunocytochemical localization of L-glutamate decarboxylase (GAD), the enzyme which which forms gamma-aminobutyric acid (GABA), has been studied in developing rodent cerebellum. During the first 3-4 postnatal days, GAD is distributed along non-terminal portions of axonal processes in close association with small vesicles. Some of the axonal processes emanate from profiles which resemble growth cone varicosities, and are presumed to be foliopodia which extend distally from axonal growth regions. At the end of the first postnatal week the GAD-containing axonal processes are seen to form protosynaptic contacts, and GAD is localized around synaptic vesicles and at presynaptic junctional membranes. During the second and third postnatal weeks GAD gradually becomes localized to mature synaptic terminals in association with synaptic vesicle, mitochondrial, and presynaptic junctional membranes. The results suggest that GAD is present in growing neurites in close association with small vesicles prior to the time the neurites make protosynaptic contacts, and that differentiation of these contacts results in a sequestering of GAD into synaptic terminals.", "contents": "The fine structural localization of glutamate decarboxylase in developing axonal processes and presynaptic terminals of rodent cerebellum. The immunocytochemical localization of L-glutamate decarboxylase (GAD), the enzyme which which forms gamma-aminobutyric acid (GABA), has been studied in developing rodent cerebellum. During the first 3-4 postnatal days, GAD is distributed along non-terminal portions of axonal processes in close association with small vesicles. Some of the axonal processes emanate from profiles which resemble growth cone varicosities, and are presumed to be foliopodia which extend distally from axonal growth regions. At the end of the first postnatal week the GAD-containing axonal processes are seen to form protosynaptic contacts, and GAD is localized around synaptic vesicles and at presynaptic junctional membranes. During the second and third postnatal weeks GAD gradually becomes localized to mature synaptic terminals in association with synaptic vesicle, mitochondrial, and presynaptic junctional membranes. The results suggest that GAD is present in growing neurites in close association with small vesicles prior to the time the neurites make protosynaptic contacts, and that differentiation of these contacts results in a sequestering of GAD into synaptic terminals."}
{"id": "PMID:1089461", "title": "Clinical and clinico-pathological effects of Escherichia coli endotoxin in mature cattle.", "content": "The effects of intravenous administration of Escherichia coli endotoxin were studied in eight mature lactating cows. Three cows were studied following intrammary infection with E. coli. Significant clinical findings are presented. Significant clinico-pathological findings include leukopenia, decreased blood serum calcium concentrations and increased levels of serum glutamic-oxaloacetic transaminase and serum ornithine-carbamyl transferase. Significant elevations of plasma corticosteroids were also noted.", "contents": "Clinical and clinico-pathological effects of Escherichia coli endotoxin in mature cattle. The effects of intravenous administration of Escherichia coli endotoxin were studied in eight mature lactating cows. Three cows were studied following intrammary infection with E. coli. Significant clinical findings are presented. Significant clinico-pathological findings include leukopenia, decreased blood serum calcium concentrations and increased levels of serum glutamic-oxaloacetic transaminase and serum ornithine-carbamyl transferase. Significant elevations of plasma corticosteroids were also noted."}
{"id": "PMID:1089462", "title": "The effect of antisera on porcine enteropathogenic Escherichia coli in ligated segments of pig intestine.", "content": "Nineteen antisera produced in pigs against 14 enteropathogenic and five nonenterotoxigenic porcine strains of Escherichia coli were tested for their ability to inhibit gut loop fluid accumulation induced by homologous and heterologous organisms. In addition, four antisera produced in pigs by an intensive series of intravenous inoculations and three by a less intensive series of intramuscular injections of a polyvalent E. coli vaccine were evaluated. Antisera were also produced in rabbits against eight strains of porcine enteropathogens and tested in pig gut loops. Fluid inhibiting activity was detected in prevaccinal sera of pigs but not of rabbits. This activity was significantly increased following immunization. When single strains of E. coli were used for immunization the activity of the antisera against heterologous organisms varied considerably from one test strain to another and was usually much less than that against the homologous organism. The activity against heterologous organisms could not be associated with relatedness of the O, K and H antigens of the vaccine and the test strains. Antisera produced against a vaccine made by combining three strains were shown to exert inhibitory effects on heterologous organisms similar to those against homologous organisms. Considerably less activity against homologous and heterologous organisms was present in antisera produced by the series of intramuscular compared with the series of intravenous injections.", "contents": "The effect of antisera on porcine enteropathogenic Escherichia coli in ligated segments of pig intestine. Nineteen antisera produced in pigs against 14 enteropathogenic and five nonenterotoxigenic porcine strains of Escherichia coli were tested for their ability to inhibit gut loop fluid accumulation induced by homologous and heterologous organisms. In addition, four antisera produced in pigs by an intensive series of intravenous inoculations and three by a less intensive series of intramuscular injections of a polyvalent E. coli vaccine were evaluated. Antisera were also produced in rabbits against eight strains of porcine enteropathogens and tested in pig gut loops. Fluid inhibiting activity was detected in prevaccinal sera of pigs but not of rabbits. This activity was significantly increased following immunization. When single strains of E. coli were used for immunization the activity of the antisera against heterologous organisms varied considerably from one test strain to another and was usually much less than that against the homologous organism. The activity against heterologous organisms could not be associated with relatedness of the O, K and H antigens of the vaccine and the test strains. Antisera produced against a vaccine made by combining three strains were shown to exert inhibitory effects on heterologous organisms similar to those against homologous organisms. Considerably less activity against homologous and heterologous organisms was present in antisera produced by the series of intramuscular compared with the series of intravenous injections."}
{"id": "PMID:1089463", "title": "A mixed bacterial population in a continuous culture with and without kaolinite.", "content": "Chromobacterium lividum and a Pseudomonas sp. were grown in pure and mixed continuous culture with and without the clay-mineral, kaolinite. Irrespective of the growth conditions, C. lividum adhered to the wall of the culture vessel whereas the Pseudomonas sp. showed no such tendency, at least visually. During mixed culture studies, the organism which was initially established in the culture dominated. The ratio between C. lividum and the Pseudomonas sp. was about 20:1 when C. lividum was first established and 1:2 when the Pseudomonas sp. was first grown. The indirect fluorescent antibody technique provided a rapid method for differentiating the mixed cultures when the bacterial concentration was sufficient for microscopic analysis. During both pure and mixed continuous culture studies, the addition of kaolinite reduced the C. lividum but not the Pseudomonas sp. population.", "contents": "A mixed bacterial population in a continuous culture with and without kaolinite. Chromobacterium lividum and a Pseudomonas sp. were grown in pure and mixed continuous culture with and without the clay-mineral, kaolinite. Irrespective of the growth conditions, C. lividum adhered to the wall of the culture vessel whereas the Pseudomonas sp. showed no such tendency, at least visually. During mixed culture studies, the organism which was initially established in the culture dominated. The ratio between C. lividum and the Pseudomonas sp. was about 20:1 when C. lividum was first established and 1:2 when the Pseudomonas sp. was first grown. The indirect fluorescent antibody technique provided a rapid method for differentiating the mixed cultures when the bacterial concentration was sufficient for microscopic analysis. During both pure and mixed continuous culture studies, the addition of kaolinite reduced the C. lividum but not the Pseudomonas sp. population."}
{"id": "PMID:1089466", "title": "Massive pulmonary gangrene: a severe complication of Klebsiella pneumonia.", "content": "Massive pulmonary gangrene developed in two patients. Review of the literature reveals 10 other case reports of pulmonary gangrene complicating lobar pneumonia. Among the total of 12 patients whose cases have now been reported, all 4 patients who were treated nonsurgically died and the 8 who underwent surgical resection of the gangrenous lung survived. The present report emphasizes the necessity of early recognition and appropriate surgical treatment for a successful outcome.", "contents": "Massive pulmonary gangrene: a severe complication of Klebsiella pneumonia. Massive pulmonary gangrene developed in two patients. Review of the literature reveals 10 other case reports of pulmonary gangrene complicating lobar pneumonia. Among the total of 12 patients whose cases have now been reported, all 4 patients who were treated nonsurgically died and the 8 who underwent surgical resection of the gangrenous lung survived. The present report emphasizes the necessity of early recognition and appropriate surgical treatment for a successful outcome."}
{"id": "PMID:1089468", "title": "Pathogenesis of human cholesterol cholelithiasis.", "content": "The pathogenesis of cholesterol cholelithiasis in humans has been studied by means of three techniques. The cholesterol-solubilizing capacity of bile may be determined by estimation of the relative composition of the three major lipid constituents of bile. Consistent reduction in the cholesterol-carrying capacity of gallbladder bile of persons with gallstones when compared with normal subjects has not been shown. Normal subjects frequently have supersaturated bile. Secretion rates of biliary lipids have been estimated by two methods; with the method that appears to be more physiologic no change in lipid secretion rates was found in gallstone patients. Bile acid pool size has been measured by isotope dilution techniques; it is reduced in patients with gallstones. It is not clear whether this reduction is important in the pathogenesis of cholesterol cholelithiasis, for the bile acid secretion rate is normal because of an increased rate of cycling of the pool through the enterohepatic circulation. The role of the gallbladder in the genesis of cholesterol cholelithiasis may be more important than has been realized.", "contents": "Pathogenesis of human cholesterol cholelithiasis. The pathogenesis of cholesterol cholelithiasis in humans has been studied by means of three techniques. The cholesterol-solubilizing capacity of bile may be determined by estimation of the relative composition of the three major lipid constituents of bile. Consistent reduction in the cholesterol-carrying capacity of gallbladder bile of persons with gallstones when compared with normal subjects has not been shown. Normal subjects frequently have supersaturated bile. Secretion rates of biliary lipids have been estimated by two methods; with the method that appears to be more physiologic no change in lipid secretion rates was found in gallstone patients. Bile acid pool size has been measured by isotope dilution techniques; it is reduced in patients with gallstones. It is not clear whether this reduction is important in the pathogenesis of cholesterol cholelithiasis, for the bile acid secretion rate is normal because of an increased rate of cycling of the pool through the enterohepatic circulation. The role of the gallbladder in the genesis of cholesterol cholelithiasis may be more important than has been realized."}
{"id": "PMID:1089469", "title": "The central nervous system in childhood leukemia. II. Subacute leukoencephalopathy.", "content": "A study was performed to evaluate the clinical and histopathologic characteristics of a distinctive degenerative lesion within the central nervous system (CNS) of children with acute lymphocytic leukemia (ALL). Of the 231 patients in this study, 13 were found to have specific degenerative changes in telencephalic white matter. Morphologically, the principal changes consisted of diffuse reactive astrocytosis and multiple, noninflammatory necrotic foci, often containing varying amount of mineralized cellular debris. Clinical features common to all patients with this leukoencephalopathy were: 1) cranial irradiation of 2000 rads or more and 2) methotrexate administered systemically after irradiation. Comparison of selected clinical features of patients with and without leukoencephalopathy showed that methotrexate administered intravenously after a cumulative dose of CNS irradiation of 2000 rads or more can result in degeneration of CNS white matter in patients with ALL. Age at time of irradiation, bacterial infections, nutrition, and CNS leukemia were not causally related to the development of this disease. This study suggests that chemotherapeutic agents may diffuse through the blood-brain barrier following CNS irradiation of 2000 rads or more.", "contents": "The central nervous system in childhood leukemia. II. Subacute leukoencephalopathy. A study was performed to evaluate the clinical and histopathologic characteristics of a distinctive degenerative lesion within the central nervous system (CNS) of children with acute lymphocytic leukemia (ALL). Of the 231 patients in this study, 13 were found to have specific degenerative changes in telencephalic white matter. Morphologically, the principal changes consisted of diffuse reactive astrocytosis and multiple, noninflammatory necrotic foci, often containing varying amount of mineralized cellular debris. Clinical features common to all patients with this leukoencephalopathy were: 1) cranial irradiation of 2000 rads or more and 2) methotrexate administered systemically after irradiation. Comparison of selected clinical features of patients with and without leukoencephalopathy showed that methotrexate administered intravenously after a cumulative dose of CNS irradiation of 2000 rads or more can result in degeneration of CNS white matter in patients with ALL. Age at time of irradiation, bacterial infections, nutrition, and CNS leukemia were not causally related to the development of this disease. This study suggests that chemotherapeutic agents may diffuse through the blood-brain barrier following CNS irradiation of 2000 rads or more."}
{"id": "PMID:1089470", "title": "Non-Hodgkin's lymphoma: patterns of relapse from complete remission after combination chemotherapy.", "content": "An analysis of patterns of relapse from complete remission in patients with non-Hodgkin's lymphoma treated with combination chemotherapy has demonstrated differences between histologic subgroups. Patients with diffuse histiocytic lymphoma who achieved a histologically proven complete remission after 6 months of treatment without maintenance have remained disease-free whereas those with nodular poorly differentiated lymphocytic and diffuse well-differentiated lymphocytic lymphomas have demonstrated a pattern of continuous late recurrence. The initial sites of relapse from complete remission in lymphocytic lymphoma were lymph nodes and bone marrow which were involved prior to treatment. Aggressive attempts at remission induction appear warranted in patients with diffuse histiocytic lymphoma because of the potential for extended disease-free survival. Patients with nodular poorly differentiated lymphocytic lymphoma may benefit from the use of maintenance chemotherapy, or radiotherapy to regions of previously known involvement after initiation of remission with chemotherapy.", "contents": "Non-Hodgkin's lymphoma: patterns of relapse from complete remission after combination chemotherapy. An analysis of patterns of relapse from complete remission in patients with non-Hodgkin's lymphoma treated with combination chemotherapy has demonstrated differences between histologic subgroups. Patients with diffuse histiocytic lymphoma who achieved a histologically proven complete remission after 6 months of treatment without maintenance have remained disease-free whereas those with nodular poorly differentiated lymphocytic and diffuse well-differentiated lymphocytic lymphomas have demonstrated a pattern of continuous late recurrence. The initial sites of relapse from complete remission in lymphocytic lymphoma were lymph nodes and bone marrow which were involved prior to treatment. Aggressive attempts at remission induction appear warranted in patients with diffuse histiocytic lymphoma because of the potential for extended disease-free survival. Patients with nodular poorly differentiated lymphocytic lymphoma may benefit from the use of maintenance chemotherapy, or radiotherapy to regions of previously known involvement after initiation of remission with chemotherapy."}
{"id": "PMID:1089474", "title": "The role of chemotherapy in the management of soft tissue sarcomas.", "content": "Prior to the use of chemotherapy, survival for rhabdomyosarcoma which had been completely resected was 50-60%. A controled study done by Children's Cancer Study Group showed the effectiveness of combined chemotherapy used as part of primary therapy in similar patients. Children who received actinomycin D (Act D) and vincristine (Vcr) for 1 year after surgery and radiotherapy had a lower metastatic rate than those who received none. This combination resulted in an 89% survival in patients with localized disease which was surgically resectable. Patients with microscopic residual disease had a 91% survival. With more aggressive use of combined chemotherapy, experience has accumulated demonstrating improved response and survival in both localized and gross residual disease. Combinations of Act D, Vcr, and cyclophosphamide can reduce initial tumor size, making large tumor masses more amenable to surgery and radiotherapy. Currently, an intergroup study is testing the response to four different drug combinations and the duration of therapy needed for various stages of the disease.", "contents": "The role of chemotherapy in the management of soft tissue sarcomas. Prior to the use of chemotherapy, survival for rhabdomyosarcoma which had been completely resected was 50-60%. A controled study done by Children's Cancer Study Group showed the effectiveness of combined chemotherapy used as part of primary therapy in similar patients. Children who received actinomycin D (Act D) and vincristine (Vcr) for 1 year after surgery and radiotherapy had a lower metastatic rate than those who received none. This combination resulted in an 89% survival in patients with localized disease which was surgically resectable. Patients with microscopic residual disease had a 91% survival. With more aggressive use of combined chemotherapy, experience has accumulated demonstrating improved response and survival in both localized and gross residual disease. Combinations of Act D, Vcr, and cyclophosphamide can reduce initial tumor size, making large tumor masses more amenable to surgery and radiotherapy. Currently, an intergroup study is testing the response to four different drug combinations and the duration of therapy needed for various stages of the disease."}
{"id": "PMID:1089475", "title": "Role of therapeutic radiology in cancer of bone.", "content": "Management of patients with Ewing's sarcoma has been discussed with reference to the need to achieve an excellent local result from radiation therapy now that patients are experiencing long-term survival. The technique of biopsy is important: small scar; located within treatment portal; positioning such that there is a good connective tissue bed (e.g. not immediately overlying bone); tissue obtained from soft tissue component and no cortical bone removed. Radiation therapy technique is based upon: special shaping of the irradiated volume to correspond to estimate distribution of tumor; distribution of dose in accordance with tumor cell number (progressive field reduction); precision setup of patient each day; and high total dose (6000-6600 rads in 6-6.6 weeks). This treatment is to be given in combination with multidrug multicycle chemotherapy. For osteosarcoma, special attention was given to lesions of mandible, where preoperative irradiation and hemimandibulectomy appears to be much more effective than surgery alone.", "contents": "Role of therapeutic radiology in cancer of bone. Management of patients with Ewing's sarcoma has been discussed with reference to the need to achieve an excellent local result from radiation therapy now that patients are experiencing long-term survival. The technique of biopsy is important: small scar; located within treatment portal; positioning such that there is a good connective tissue bed (e.g. not immediately overlying bone); tissue obtained from soft tissue component and no cortical bone removed. Radiation therapy technique is based upon: special shaping of the irradiated volume to correspond to estimate distribution of tumor; distribution of dose in accordance with tumor cell number (progressive field reduction); precision setup of patient each day; and high total dose (6000-6600 rads in 6-6.6 weeks). This treatment is to be given in combination with multidrug multicycle chemotherapy. For osteosarcoma, special attention was given to lesions of mandible, where preoperative irradiation and hemimandibulectomy appears to be much more effective than surgery alone."}
{"id": "PMID:1089476", "title": "Treatment of lymphoma.", "content": "Lymphomatous diseases (non-Hodgkin's) of children differ markedly from those of adults in histology, natural history, and response to therapy. Information obtained from treating adults with lymphoma cannot be applied to children with equal success; the progress in treating children has been slow. The following types of non-Hodgkin's lymphoma have been distinguished in children seen at the M. D. Anderson Hospital and Tumor Institute in the past 7 years: 1) diffuse undifferentiated lymphoma (Burkitt's lymphoma); 2) diffuse undifferentiated lymphoma (non-Burkitt's lymphoma lacking leukemic propensity); 3) diffuse poorly differentiated lymphocytic lymphoma (non-Burkitt's lymphoma with leukemic propensity (convoluted cell type); and 4) diffuse histiocytic lymphoma (histiocytic lymphoma). The interrelationships of age, sex, histology, and primary site are presented. The occurrence of mediastinal masses in non-Burkitt's tumor (convoluted cell type) is particularly striking. Therapeutic regimens designed for specific types of non-Hodgkin's lymphoma have improved the outcome of treatment in Burkitt's lymphoma, non-Burkitt's lymphoma (convoluted cell type), and histiocytic lymphoma (Stages I and II) as compared with treatment regimens used prior to 1967.", "contents": "Treatment of lymphoma. Lymphomatous diseases (non-Hodgkin's) of children differ markedly from those of adults in histology, natural history, and response to therapy. Information obtained from treating adults with lymphoma cannot be applied to children with equal success; the progress in treating children has been slow. The following types of non-Hodgkin's lymphoma have been distinguished in children seen at the M. D. Anderson Hospital and Tumor Institute in the past 7 years: 1) diffuse undifferentiated lymphoma (Burkitt's lymphoma); 2) diffuse undifferentiated lymphoma (non-Burkitt's lymphoma lacking leukemic propensity); 3) diffuse poorly differentiated lymphocytic lymphoma (non-Burkitt's lymphoma with leukemic propensity (convoluted cell type); and 4) diffuse histiocytic lymphoma (histiocytic lymphoma). The interrelationships of age, sex, histology, and primary site are presented. The occurrence of mediastinal masses in non-Burkitt's tumor (convoluted cell type) is particularly striking. Therapeutic regimens designed for specific types of non-Hodgkin's lymphoma have improved the outcome of treatment in Burkitt's lymphoma, non-Burkitt's lymphoma (convoluted cell type), and histiocytic lymphoma (Stages I and II) as compared with treatment regimens used prior to 1967."}
{"id": "PMID:1089491", "title": "Effect of digoxin on atioventricular conduction. Studies in patients with and without cardiac autonomic innervation.", "content": "The effect of digoxin on atrioventricular (a-v) conduction was compared in five patients with an intact cardiac autonomic nervous system (Group I) and seven patients who had undergone cardiac transplantation (Group II), in whom we have previously shown the transplanted heart to be completely denervated. Small decreases in the atrial effective refractory period (ERP) (from 262 plus or minus 12 to 254 plus or minus 11 msec) and atrial functional refractory period (FRP) (from 304 plus or minus 12 msec) were observed in Group I patients after digoxin, but these changes were not significant. However, significant increases in the A-V nodal ERP (from 315 plus or minus 18 msec to 351 plus or minus 17 msec, P less than 0.05), and A-V nodal FRP (from 426 plus or minus 42 to 460 plus or minus 46 msec, P less than 0.01) were produced by digoxin and were unrelated to changes in cycle length. In Group II patients with denervated hearts, changes in atrial ERP (from 246 plus or minus 4 to 243 plus or minus 6 during spontaneous sinus rhythm; from 204 plus or minus 10 to 216 plus or minus 8 msec during atrial pacing) and atrial FRP (from 311 plus or minus 12 to 316 plus or minus 11 msec during spontaneous sinus rhythm; from 254 plus or minus 12 to 260 plus or minus 10 msec during atrial pacing) were not significant. However, in contrast to the Group I patients, the digoxin-induced changes in A-V nodal ERP (from 280 plus or minus 22 to 297 plus or minus 18 msec during atrial pacing) and FRP (from 368 plus or minus 18 to 377 plus or minus 18 msec during spontaneous sinus rhythm; from 334 plus or minus 13 to 346 plus or minus 16 msec during atrial pacing) were also statistically insignificant. Our results demonstrate that the electrophysiologic effects of digoxin on atrioventricular conduction in man are most marked in the atrioventricular node and are dependent on cardiac innervation", "contents": "Effect of digoxin on atioventricular conduction. Studies in patients with and without cardiac autonomic innervation. The effect of digoxin on atrioventricular (a-v) conduction was compared in five patients with an intact cardiac autonomic nervous system (Group I) and seven patients who had undergone cardiac transplantation (Group II), in whom we have previously shown the transplanted heart to be completely denervated. Small decreases in the atrial effective refractory period (ERP) (from 262 plus or minus 12 to 254 plus or minus 11 msec) and atrial functional refractory period (FRP) (from 304 plus or minus 12 msec) were observed in Group I patients after digoxin, but these changes were not significant. However, significant increases in the A-V nodal ERP (from 315 plus or minus 18 msec to 351 plus or minus 17 msec, P less than 0.05), and A-V nodal FRP (from 426 plus or minus 42 to 460 plus or minus 46 msec, P less than 0.01) were produced by digoxin and were unrelated to changes in cycle length. In Group II patients with denervated hearts, changes in atrial ERP (from 246 plus or minus 4 to 243 plus or minus 6 during spontaneous sinus rhythm; from 204 plus or minus 10 to 216 plus or minus 8 msec during atrial pacing) and atrial FRP (from 311 plus or minus 12 to 316 plus or minus 11 msec during spontaneous sinus rhythm; from 254 plus or minus 12 to 260 plus or minus 10 msec during atrial pacing) were not significant. However, in contrast to the Group I patients, the digoxin-induced changes in A-V nodal ERP (from 280 plus or minus 22 to 297 plus or minus 18 msec during atrial pacing) and FRP (from 368 plus or minus 18 to 377 plus or minus 18 msec during spontaneous sinus rhythm; from 334 plus or minus 13 to 346 plus or minus 16 msec during atrial pacing) were also statistically insignificant. Our results demonstrate that the electrophysiologic effects of digoxin on atrioventricular conduction in man are most marked in the atrioventricular node and are dependent on cardiac innervation"}
{"id": "PMID:1089492", "title": "The potential of phosphorescence spectrometry in clinical chemistry--the new generation of instrumentation and methodology.", "content": "We review recent advances in instrumentation and methodology in phosphorimetry that should facilitate the use of phosphorimetry for clinical analyses and recent phosphorescence studies of interest to the clinical chemist. We indicate recent advances, particularly improvements in instrumentation, novel methodologies, and new chemical processes that result in either an increase in sensitivity or selectivity (or both) of measurement of compounds of clinical importance. The greatest use of phosphorimetry in the clinical laboratory will not be for the analysis of very large numbers of samples for one species via automatic instrumentation, but rather will be for the analysis of those molecular species difficult or impossible to measure by conventional methods (colorimetry, fluorometry, etc.). Although various instrumental and methodological advances are discussed separately here, the most important use of these advances in clinical chemistry will undoubtedly be when two or more of them are combined, for example, in the use of time- or frequency-resolved phosphorimetry for the selective measurement of the phosphorescence resulting with inorganic probes and the appropriate choice of solvent and pH (of course, the instrument could contain an image vidicon detector for rapid determination of the spectrum, the decay curve, or both).", "contents": "The potential of phosphorescence spectrometry in clinical chemistry--the new generation of instrumentation and methodology. We review recent advances in instrumentation and methodology in phosphorimetry that should facilitate the use of phosphorimetry for clinical analyses and recent phosphorescence studies of interest to the clinical chemist. We indicate recent advances, particularly improvements in instrumentation, novel methodologies, and new chemical processes that result in either an increase in sensitivity or selectivity (or both) of measurement of compounds of clinical importance. The greatest use of phosphorimetry in the clinical laboratory will not be for the analysis of very large numbers of samples for one species via automatic instrumentation, but rather will be for the analysis of those molecular species difficult or impossible to measure by conventional methods (colorimetry, fluorometry, etc.). Although various instrumental and methodological advances are discussed separately here, the most important use of these advances in clinical chemistry will undoubtedly be when two or more of them are combined, for example, in the use of time- or frequency-resolved phosphorimetry for the selective measurement of the phosphorescence resulting with inorganic probes and the appropriate choice of solvent and pH (of course, the instrument could contain an image vidicon detector for rapid determination of the spectrum, the decay curve, or both)."}
{"id": "PMID:1089504", "title": "Intramammary infections in the sow during the peripartum period.", "content": "The incidence and ype of aerobic bacteria causing intramammary infection (IMI) in two groups of peripartum sows were determined. The incidence of IMI in 24 sows near farrowing was 30.2 percent; the incidence in 16 machine-milked sows was 81.0 percent. Numerous mixed IMI were detected. The bacterium causing most IMI was Staphylococcus epidermidis, followed by beta hemolytic streptococci, aslpha hemolytic streptococci, Escherichia coli, and diphtheroids. The beta hemolytic streptococci and E. coli cultures were studied in detail. The relationship of bacterial IMI to agalactiae toxemia is discussed.", "contents": "Intramammary infections in the sow during the peripartum period. The incidence and ype of aerobic bacteria causing intramammary infection (IMI) in two groups of peripartum sows were determined. The incidence of IMI in 24 sows near farrowing was 30.2 percent; the incidence in 16 machine-milked sows was 81.0 percent. Numerous mixed IMI were detected. The bacterium causing most IMI was Staphylococcus epidermidis, followed by beta hemolytic streptococci, aslpha hemolytic streptococci, Escherichia coli, and diphtheroids. The beta hemolytic streptococci and E. coli cultures were studied in detail. The relationship of bacterial IMI to agalactiae toxemia is discussed."}
{"id": "PMID:1089505", "title": "Surgical treatment of Candida endocarditis.", "content": "Case reports from a group of 15 patients with Candida endocarditis seen and treated at the Los Angeles-University of Southern California Medical Center between 1960 and 1974, together with a survey of other reported cases, serve to re-emphasize the importance of early diagnosis of this disease and lead to certain conclusions regarding its treatment. With medical or surgical treatment alone, the mortality for Candida endocarditis is 82 per cent. With surgery and medical treatment combined, the mortality is 20 percent. It is important to institute medical treatment as soon as diagnosis of Candida endocarditis is made and surgery must be performed as soon as possible, preferably within 24 to 48 hours or, at most, a few days after the patient has left the hospital. Close observation of the patient after discharge is important.", "contents": "Surgical treatment of Candida endocarditis. Case reports from a group of 15 patients with Candida endocarditis seen and treated at the Los Angeles-University of Southern California Medical Center between 1960 and 1974, together with a survey of other reported cases, serve to re-emphasize the importance of early diagnosis of this disease and lead to certain conclusions regarding its treatment. With medical or surgical treatment alone, the mortality for Candida endocarditis is 82 per cent. With surgery and medical treatment combined, the mortality is 20 percent. It is important to institute medical treatment as soon as diagnosis of Candida endocarditis is made and surgery must be performed as soon as possible, preferably within 24 to 48 hours or, at most, a few days after the patient has left the hospital. Close observation of the patient after discharge is important."}
{"id": "PMID:1089506", "title": "Increased sensitivity of regional measurements in early detection of narcotic lung disease.", "content": "Intravascular foreign body thromboembolism and interstitial or vascular granulomata in the lungs are common sequelae to intravenous drug abuse. Early detection of such complications is important, because of the youthful population often involved. Conventional pulmonary function tests, including forced expiratory volumes, diffusion capacity for CO (DLco), chest radiograph and arterial blood gas analysis were performed in 23 narcotic addicts, most of whom had no pulmonary symptoms. The results were compared with radionuclide pulmonary function studies obtained following inhalation of 133Xe gas and injection of 99-mTc albumin microparticles. Thirteen subjects who had normal nonnuclear pulmonary function tests, including DLco and chest radiograph, demonstrated significant abnormalities of regional lung perfusion by lung scan. Of the ten subjects with abnormal DLco all had abnormal perfusion scans and five were noted to have hypoxia at rest. In addition, six of the ten subjects with abnormal DLco revealed a history of concomitant \"solid pill abuse\" along with heroin abuse, whereas only 2 of 13 subjects with normal DLco admitted tooccasional solid pill abuse. The perfusion lung scan is therefore a sensitive means for detection of early lung disease in narcotic addicts.", "contents": "Increased sensitivity of regional measurements in early detection of narcotic lung disease. Intravascular foreign body thromboembolism and interstitial or vascular granulomata in the lungs are common sequelae to intravenous drug abuse. Early detection of such complications is important, because of the youthful population often involved. Conventional pulmonary function tests, including forced expiratory volumes, diffusion capacity for CO (DLco), chest radiograph and arterial blood gas analysis were performed in 23 narcotic addicts, most of whom had no pulmonary symptoms. The results were compared with radionuclide pulmonary function studies obtained following inhalation of 133Xe gas and injection of 99-mTc albumin microparticles. Thirteen subjects who had normal nonnuclear pulmonary function tests, including DLco and chest radiograph, demonstrated significant abnormalities of regional lung perfusion by lung scan. Of the ten subjects with abnormal DLco all had abnormal perfusion scans and five were noted to have hypoxia at rest. In addition, six of the ten subjects with abnormal DLco revealed a history of concomitant \"solid pill abuse\" along with heroin abuse, whereas only 2 of 13 subjects with normal DLco admitted tooccasional solid pill abuse. The perfusion lung scan is therefore a sensitive means for detection of early lung disease in narcotic addicts."}
{"id": "PMID:1089507", "title": "Pulmonary function studies in ex-heroin users.", "content": "Pulmonary function tests were performed on a group of 22-ex-heroin addicts with no known pulmonary disease or symptoms. Vital capacity and total lung capacity were normal in all but four patients. Only two patients had evidence of moderately severe airway obstruction. However, the single breath diffusing capacity (Dco) was significantly reduced in many of the patients and averaged 81 percent of predicted for the entire group. Patients with most severe reduction of Dco also had reduction of lung volumes. In addition, six patients had increased lung recoil force (Pmax) and decreased lung compliance. There was no correlation between degree of functional impairment and duration or amount of durg use, incidence of overdose or intravenous injection of crushed tablets. Followup studies in four patients after several months on methadone maintenance showed improvement in lung volumes and/or Dco. Possible etiologic considerations to explain these abnormalities were previous subclinical pneumonias, foreign body granulomas, previous pulmonary edema or other direct toxic effect on alveolocapillary membrane as the result of use of drugs.", "contents": "Pulmonary function studies in ex-heroin users. Pulmonary function tests were performed on a group of 22-ex-heroin addicts with no known pulmonary disease or symptoms. Vital capacity and total lung capacity were normal in all but four patients. Only two patients had evidence of moderately severe airway obstruction. However, the single breath diffusing capacity (Dco) was significantly reduced in many of the patients and averaged 81 percent of predicted for the entire group. Patients with most severe reduction of Dco also had reduction of lung volumes. In addition, six patients had increased lung recoil force (Pmax) and decreased lung compliance. There was no correlation between degree of functional impairment and duration or amount of durg use, incidence of overdose or intravenous injection of crushed tablets. Followup studies in four patients after several months on methadone maintenance showed improvement in lung volumes and/or Dco. Possible etiologic considerations to explain these abnormalities were previous subclinical pneumonias, foreign body granulomas, previous pulmonary edema or other direct toxic effect on alveolocapillary membrane as the result of use of drugs."}
{"id": "PMID:1089508", "title": "The use of spontaneous ventilation with constant-positive airway pressure in the treatment of salt water near drowning.", "content": "Constant-positive airway pressure with spontaneous ventilation was successfully used in treatment of a patient who had suffered near-drowning in salt water. The patient was able to maintain adequate respiratory mechanics, although oxygenation without end-expiratory pressure was severely impaired. The rapid improvement in arterial oxygenation obviated the need for mechanical ventilation in this patient. It is suggested that the simplest modality of effective therapy should be used in such patients.", "contents": "The use of spontaneous ventilation with constant-positive airway pressure in the treatment of salt water near drowning. Constant-positive airway pressure with spontaneous ventilation was successfully used in treatment of a patient who had suffered near-drowning in salt water. The patient was able to maintain adequate respiratory mechanics, although oxygenation without end-expiratory pressure was severely impaired. The rapid improvement in arterial oxygenation obviated the need for mechanical ventilation in this patient. It is suggested that the simplest modality of effective therapy should be used in such patients."}
{"id": "PMID:1089516", "title": "[Bacteriological study of redon drains in accident surgery (author's transl)].", "content": "After 'clean' operations, 121 Redon drains from 80 patients were cultured. 17.4% of Redon tips were contaminated, but microorganisms were found in only 7.4% of wound secretions aspirated from them before they were removed. Only Gram-positive cocci, most of them of questionable pathogenicity, were found. Although drains or wound secretions were found to be contaminated in 21 patients, clinical evidence of disturbed healing occurred in only two patients. The presence of microorganisms provided no pointer to later healing difficulties. Culturing Redon tips is, therefore not a reliable method for early recognition of wound infections. Antibiotics should not be used merely on evidence of bacterial contamination of Redon tips without any clinical signs of infection.", "contents": "[Bacteriological study of redon drains in accident surgery (author's transl)]. After 'clean' operations, 121 Redon drains from 80 patients were cultured. 17.4% of Redon tips were contaminated, but microorganisms were found in only 7.4% of wound secretions aspirated from them before they were removed. Only Gram-positive cocci, most of them of questionable pathogenicity, were found. Although drains or wound secretions were found to be contaminated in 21 patients, clinical evidence of disturbed healing occurred in only two patients. The presence of microorganisms provided no pointer to later healing difficulties. Culturing Redon tips is, therefore not a reliable method for early recognition of wound infections. Antibiotics should not be used merely on evidence of bacterial contamination of Redon tips without any clinical signs of infection."}
{"id": "PMID:1089521", "title": "[Effect of aprotinin in acute myocardial infarction (clinical double-bind study (author's transl)].", "content": "In a double-bind study 164 patients with acute myocardial infarction, admitted to an intensive-care unit within 72 hours of infarction, were randomly treated for three days either with aprotinin (Trasylol-82 persons) or placebo (82 persons). Retrospective analysis proved both patient groups to have been comparable in all other respects. There was no definite influence on the course of the infarction, haemodynamic changes, arrythmias or mortality rate. Trasylol thus has no therapeutic importance in the treatment of acute myocardial infarction.", "contents": "[Effect of aprotinin in acute myocardial infarction (clinical double-bind study (author's transl)]. In a double-bind study 164 patients with acute myocardial infarction, admitted to an intensive-care unit within 72 hours of infarction, were randomly treated for three days either with aprotinin (Trasylol-82 persons) or placebo (82 persons). Retrospective analysis proved both patient groups to have been comparable in all other respects. There was no definite influence on the course of the infarction, haemodynamic changes, arrythmias or mortality rate. Trasylol thus has no therapeutic importance in the treatment of acute myocardial infarction."}
{"id": "PMID:1089522", "title": "[Clinical studies on the effect of drugs used in the treatment of angina].", "content": "The report is based on a four-centre study on 25 patients with coronary insufficiency (ECG signs). In random sequence the following were given to each patient: a nitrate (isosorbide dinitrate), carbocromene, practolol, and oxyphedrine. The initial criterion for inclusion was S-T depression of 0.2 mV on stepwise exercise (bicycle ergometry). S-T depression, systolic blood pressure and heart rate changes were used as criteria. For each drug there was an acute test and a long-term test of 12 days. The Friedman test was used for the statistical evaluation of the results. Isosorbide dinitrate decreased the extent of S-T depression in the acute tests while practolol did so in the long-term test. Practolol reduced systolic pressure on long-term administration, with no difference in the acute experiment. Heart rate was reduced by practolol both acutely and long-term. These results give no indication for any possible differential treatment of coronary insufficiency, but this may be due to the selection of patients in this study.", "contents": "[Clinical studies on the effect of drugs used in the treatment of angina]. The report is based on a four-centre study on 25 patients with coronary insufficiency (ECG signs). In random sequence the following were given to each patient: a nitrate (isosorbide dinitrate), carbocromene, practolol, and oxyphedrine. The initial criterion for inclusion was S-T depression of 0.2 mV on stepwise exercise (bicycle ergometry). S-T depression, systolic blood pressure and heart rate changes were used as criteria. For each drug there was an acute test and a long-term test of 12 days. The Friedman test was used for the statistical evaluation of the results. Isosorbide dinitrate decreased the extent of S-T depression in the acute tests while practolol did so in the long-term test. Practolol reduced systolic pressure on long-term administration, with no difference in the acute experiment. Heart rate was reduced by practolol both acutely and long-term. These results give no indication for any possible differential treatment of coronary insufficiency, but this may be due to the selection of patients in this study."}
{"id": "PMID:1089535", "title": "Inhibition of glucagon and insulin secretion by somatostatin in the rat pancreas perfused in situ.", "content": "Perfusion of growth hormone inhibitory factor (somatostatin) into rat pancreas inhibited secretion of glucagon and insulin into medium containing 5.5 mM glucose. A 15-min infusion of arginine (20 mM) greatly increased glucagon and insulin secretion. When perfused simultaneously with arginine, somatostatin (55 nM) abolished the increase in glucagon secretion. The acute phase of insulin secretion in response to arginine was attenuated by somatostatin, and subsequent secretion was decreased to control levels. Pretreatment for 5 min with somatostatin blocked even acute-phase insulin secretion in response to arginine. Somatostatin did not affect basal or glucose-stimulated secretion of insulin from rat pancreatic islets isolated by the collagenase technique. Arginine-stimulated secretion of insulin was enhanced by somatostatin in isolated islets. These results demonstrate a direct effect of somatostatin on the pancreas to inhibit secretion of glucagon and insulin. The failure of somatostatin to inhibit insulin secretion in pancreatic islets may be due to alterations in the beta cells produced by the isolation procedure. It is also possible that the effect of somatostatin on insulin secretion may be mediated indirectly.", "contents": "Inhibition of glucagon and insulin secretion by somatostatin in the rat pancreas perfused in situ. Perfusion of growth hormone inhibitory factor (somatostatin) into rat pancreas inhibited secretion of glucagon and insulin into medium containing 5.5 mM glucose. A 15-min infusion of arginine (20 mM) greatly increased glucagon and insulin secretion. When perfused simultaneously with arginine, somatostatin (55 nM) abolished the increase in glucagon secretion. The acute phase of insulin secretion in response to arginine was attenuated by somatostatin, and subsequent secretion was decreased to control levels. Pretreatment for 5 min with somatostatin blocked even acute-phase insulin secretion in response to arginine. Somatostatin did not affect basal or glucose-stimulated secretion of insulin from rat pancreatic islets isolated by the collagenase technique. Arginine-stimulated secretion of insulin was enhanced by somatostatin in isolated islets. These results demonstrate a direct effect of somatostatin on the pancreas to inhibit secretion of glucagon and insulin. The failure of somatostatin to inhibit insulin secretion in pancreatic islets may be due to alterations in the beta cells produced by the isolation procedure. It is also possible that the effect of somatostatin on insulin secretion may be mediated indirectly."}
{"id": "PMID:1089536", "title": "Subcellular localization of LH releasing activity in the rat hypothalamus.", "content": "Separation of particulate fractions associated with LH releasing activity has been effected by differential centrifugation followed by sucrose density gradient centrifugation. The fractions were monitored for LH releasing activity by an in vitro incubation with rat pituitaries and for subcellular organelles by electron microscopy. LH released into the incubation medium and contained in the pituitaries was measured by radioimmunoassay (RIA). After differential centrifugation only the \"crude mitochondrial fraction\" caused an increase of LH release accompanied by a depletion of pituitary LH and a rise in total LH. Further fractionation of this pellet on a discontinuous sucrose gradient resulted in three opaque bands and three clear areas which were removed as sic fractions. Only one fraction, not associated with myelin, synaptosomes, or mitochondria, caused an increase in LH release and total LH while pituitary LH remained unchanged. This fraction appears to be predominantly composed of electron dense vesicles of varying sizes. Also of interest is another fraction which decreased LH release and was again not associated with myelin, synaptosomes, or mitochondria. The decreased release was associated with an increased pituitary LH while total LH remained unchanged. Of the six subcellular fractions obtained from the \"crude mitochondrial pellet\" only these two caused significant changes in LH release. Two other neural tissues, the cerebellum and cerebral cortex, were similarly processed. None of these subcellular fractions significantly altered LH release and/or synthesis.", "contents": "Subcellular localization of LH releasing activity in the rat hypothalamus. Separation of particulate fractions associated with LH releasing activity has been effected by differential centrifugation followed by sucrose density gradient centrifugation. The fractions were monitored for LH releasing activity by an in vitro incubation with rat pituitaries and for subcellular organelles by electron microscopy. LH released into the incubation medium and contained in the pituitaries was measured by radioimmunoassay (RIA). After differential centrifugation only the \"crude mitochondrial fraction\" caused an increase of LH release accompanied by a depletion of pituitary LH and a rise in total LH. Further fractionation of this pellet on a discontinuous sucrose gradient resulted in three opaque bands and three clear areas which were removed as sic fractions. Only one fraction, not associated with myelin, synaptosomes, or mitochondria, caused an increase in LH release and total LH while pituitary LH remained unchanged. This fraction appears to be predominantly composed of electron dense vesicles of varying sizes. Also of interest is another fraction which decreased LH release and was again not associated with myelin, synaptosomes, or mitochondria. The decreased release was associated with an increased pituitary LH while total LH remained unchanged. Of the six subcellular fractions obtained from the \"crude mitochondrial pellet\" only these two caused significant changes in LH release. Two other neural tissues, the cerebellum and cerebral cortex, were similarly processed. None of these subcellular fractions significantly altered LH release and/or synthesis."}
{"id": "PMID:1089537", "title": "Effects of cervical stimulation and anti-LH releasing hormone serum on LH releasing hormone content in the hypothalamus.", "content": "Hypothalamic content of LHRH was radioimmunoassayed after cervical stimulation on the morning of day of estrus' LHRH content rose 3-fold by 120 min after the stimulation, reaching a level equivalent to that of day 5 of pseudopregnancy. The increase was not steady as there were two early periods when the content did not increase. Measurement of pituitary LH content revealed a marked increase followed by a sudden decrease which corresponded respectively to the two periods when LHRH levels failed ro tise. These changes in pituitary LH level suggest that both the synthesis and release of LH in the pituitary are promoted by the action of LHRH released from the hypothalamus. LH changes in the pituitary were abolished iv injection of 2 ml LHRH antiserum at the time of cervical stimulation, but pseudopregnancy was still induced and diestrus prolonged 4 days longer than in the control group. Progestin concentrations in ovarian vein blood were comparable between anti-LHRH-treated and control groups. These results suggest that the LH released by cervical stimulation is dispensable for the induction of pseudopregnancy. The marked elevation of LHRH in the hypothalamus observed immediately after cervical stimulation must be related to the regulatory mechanism of the characteristic secretory pattern of LH during the period of pseudopregnancy.", "contents": "Effects of cervical stimulation and anti-LH releasing hormone serum on LH releasing hormone content in the hypothalamus. Hypothalamic content of LHRH was radioimmunoassayed after cervical stimulation on the morning of day of estrus' LHRH content rose 3-fold by 120 min after the stimulation, reaching a level equivalent to that of day 5 of pseudopregnancy. The increase was not steady as there were two early periods when the content did not increase. Measurement of pituitary LH content revealed a marked increase followed by a sudden decrease which corresponded respectively to the two periods when LHRH levels failed ro tise. These changes in pituitary LH level suggest that both the synthesis and release of LH in the pituitary are promoted by the action of LHRH released from the hypothalamus. LH changes in the pituitary were abolished iv injection of 2 ml LHRH antiserum at the time of cervical stimulation, but pseudopregnancy was still induced and diestrus prolonged 4 days longer than in the control group. Progestin concentrations in ovarian vein blood were comparable between anti-LHRH-treated and control groups. These results suggest that the LH released by cervical stimulation is dispensable for the induction of pseudopregnancy. The marked elevation of LHRH in the hypothalamus observed immediately after cervical stimulation must be related to the regulatory mechanism of the characteristic secretory pattern of LH during the period of pseudopregnancy."}
{"id": "PMID:1089538", "title": "Hypothalamic control of the pituitary-testicular relation in fetal rats: measurement of collective volume of Leydig cells.", "content": "On the 20th day of gestation in pregnant rats, the male fetuses were subjected to surgical hypophysectomy by decapitation or to intracranial paraffin injection which compressed the fetal brain. Autopsy was done on the 22nd day of gestation. The collective volume of Leydig cells in the left fetal testis was estimated by Chalkley's method. Decapitation of a fetus caused a significant retardation in increase of the collective volume of Leydig cells 2 days later. In fetuses given an intracranial paraffin injection, the Leydig cell volume was increased significantly compared with normal fetuses on the 20th day of gestation but was far smaller than that in their intact littermates. This effect of paraffin compression was completely prevented by injections of LRH. The Leydig cell volume remained extremely small in decapitated fetuses given LRH. The observations suggest that in the male rat the hypothalamus begins to govern a pituitary gonadotropic function before birth.", "contents": "Hypothalamic control of the pituitary-testicular relation in fetal rats: measurement of collective volume of Leydig cells. On the 20th day of gestation in pregnant rats, the male fetuses were subjected to surgical hypophysectomy by decapitation or to intracranial paraffin injection which compressed the fetal brain. Autopsy was done on the 22nd day of gestation. The collective volume of Leydig cells in the left fetal testis was estimated by Chalkley's method. Decapitation of a fetus caused a significant retardation in increase of the collective volume of Leydig cells 2 days later. In fetuses given an intracranial paraffin injection, the Leydig cell volume was increased significantly compared with normal fetuses on the 20th day of gestation but was far smaller than that in their intact littermates. This effect of paraffin compression was completely prevented by injections of LRH. The Leydig cell volume remained extremely small in decapitated fetuses given LRH. The observations suggest that in the male rat the hypothalamus begins to govern a pituitary gonadotropic function before birth."}
{"id": "PMID:1089539", "title": "Absence of luteinizing hormone-releasing hormone (LH-RH) from catecholaminergic neurons.", "content": "6-Hydroxy-dopamine was administered into the third ventricle of adult male rats. After 2 successive 250 mug doses of 6-OH-dopamine, the levels of norepinephrine and dopamine in the median eminence and arcuate nucleus fell to 15-20% of the control value. The amount of luteinizing hormone releasing hormone (LH-RH) in these same hypothalamic areas, however, was unchanged. It is concluded that LH-RH containing cells are distinct from catecholaminergic neurons.", "contents": "Absence of luteinizing hormone-releasing hormone (LH-RH) from catecholaminergic neurons. 6-Hydroxy-dopamine was administered into the third ventricle of adult male rats. After 2 successive 250 mug doses of 6-OH-dopamine, the levels of norepinephrine and dopamine in the median eminence and arcuate nucleus fell to 15-20% of the control value. The amount of luteinizing hormone releasing hormone (LH-RH) in these same hypothalamic areas, however, was unchanged. It is concluded that LH-RH containing cells are distinct from catecholaminergic neurons."}
{"id": "PMID:1089540", "title": "Purification and characterization of 30-S ribosomal proteins from Bacillus stearothermophilus.", "content": "Twenty-three proteins were identified by two-dimensional eletrophoresis on polyacrylamide-gel slabs in the 30-S ribosomal subunit of Bacillus stearothermophilus strain 799. They were designated as B-S1 through B-S21, B-Sa and B-Sb and purified on carboxymethyl-cellulose and Sephadex G100 in the presence of 6 M urea. Their molecular weight was estimated by dodecyl-sulfate-gel electrophoresis and their amino acid composition was determined after acid hydrolysis. Results obtained for the individual proteins were essentially similar to those for Escherichia coli 30-S proteins with some characteristic differences.", "contents": "Purification and characterization of 30-S ribosomal proteins from Bacillus stearothermophilus. Twenty-three proteins were identified by two-dimensional eletrophoresis on polyacrylamide-gel slabs in the 30-S ribosomal subunit of Bacillus stearothermophilus strain 799. They were designated as B-S1 through B-S21, B-Sa and B-Sb and purified on carboxymethyl-cellulose and Sephadex G100 in the presence of 6 M urea. Their molecular weight was estimated by dodecyl-sulfate-gel electrophoresis and their amino acid composition was determined after acid hydrolysis. Results obtained for the individual proteins were essentially similar to those for Escherichia coli 30-S proteins with some characteristic differences."}
{"id": "PMID:1089541", "title": "Somatic antigen of Shigella dysenteriae type 3. Structural features of specific polysaccharide chain.", "content": "On mild acid hydrolysis of lipolysaccharide from Shigella dysenteriae type 3 the O-specific polysaccharide (hapten) was obtained which appeared to be acidic branched hexosaminoglycan. The repeating unit of this polysaccharide represents a pentasaccharide composed of two D-galactose residues, N-acetyl-D-galactosamine, D-glucose and unidentified acidic component. On the basis of methylation analysis, periodate oxidation, partial acid hydrolysis and chromic anhydride oxidation it is concluded that the structure of the chemical repeating unit of polysaccharide is (see article) where Glcp is glucopyranose, Galp is galactopyranose, Galf is galactofuranose, GalNAcp is 2-acetamido-2-deoxygalactopyranose and where the configuration of galactofuranoside glycosidic linkage and the structure of the acidic monosaccharide A are not known.", "contents": "Somatic antigen of Shigella dysenteriae type 3. Structural features of specific polysaccharide chain. On mild acid hydrolysis of lipolysaccharide from Shigella dysenteriae type 3 the O-specific polysaccharide (hapten) was obtained which appeared to be acidic branched hexosaminoglycan. The repeating unit of this polysaccharide represents a pentasaccharide composed of two D-galactose residues, N-acetyl-D-galactosamine, D-glucose and unidentified acidic component. On the basis of methylation analysis, periodate oxidation, partial acid hydrolysis and chromic anhydride oxidation it is concluded that the structure of the chemical repeating unit of polysaccharide is (see article) where Glcp is glucopyranose, Galp is galactopyranose, Galf is galactofuranose, GalNAcp is 2-acetamido-2-deoxygalactopyranose and where the configuration of galactofuranoside glycosidic linkage and the structure of the acidic monosaccharide A are not known."}
{"id": "PMID:1089568", "title": "Childhood mortality, family size and birth order in pre-industrial Europe.", "content": "Based on parish registers, demographic histories of Crulai (France), Tourouvre-au-Perche (France), and Geneva (Swizertland) established the childhood mortality experienced by complete sibships during periods of at least half a century before the Fench revolution. These observations may be presented as frequenceis in incomplete five-dimensional contingency tables. The five dimensions are: survival (living or dead), completed sibship size, birth order, type of family (according to completeness of information about family), and epoch (period in which the family lived). This paper reanalyzes these published data, using hierarchical log-linear models to discern which interations among the five variables can justifiably be inferred from the data. The neonatal and infant mortality rates of firstborn are probably higher than those of later sibs (in Crulai and Tourouvre). But mortality by age 20 (in Geneva) is associated strongly with the epoch, type of family, and family size, and not significantly with birth order. The increase in mortality with completed family size is insufficient to select, in an evolutionary sense, for limited family size.", "contents": "Childhood mortality, family size and birth order in pre-industrial Europe. Based on parish registers, demographic histories of Crulai (France), Tourouvre-au-Perche (France), and Geneva (Swizertland) established the childhood mortality experienced by complete sibships during periods of at least half a century before the Fench revolution. These observations may be presented as frequenceis in incomplete five-dimensional contingency tables. The five dimensions are: survival (living or dead), completed sibship size, birth order, type of family (according to completeness of information about family), and epoch (period in which the family lived). This paper reanalyzes these published data, using hierarchical log-linear models to discern which interations among the five variables can justifiably be inferred from the data. The neonatal and infant mortality rates of firstborn are probably higher than those of later sibs (in Crulai and Tourouvre). But mortality by age 20 (in Geneva) is associated strongly with the epoch, type of family, and family size, and not significantly with birth order. The increase in mortality with completed family size is insufficient to select, in an evolutionary sense, for limited family size."}
{"id": "PMID:1089575", "title": "Structure of the gastric mucosa in acute infectious bacterial gastroenteritis.", "content": "It is now well documented that a characteristic mucosal lesion of the proximal small intestine is present in acute nonbacterial gastroenteritis. To determine whether a gastric mucosal lesion also accompanies this illness, stool filtrate containing Norwalk agent was given orally to 15 volunteers after base line biopsies of gastric fundal and/or antral mucosa had been obtained. Gastric fundal and/or antral biopsies were then obtained serially between 24 and 168 hr after administration of the inoculum. Nine volunteers developed symptoms of gastroenteritis. gastric biopsies from those with normal base line fundal and/or antral biopsies remained normal during and after clinical illness. Those volunteers who had mild to moderate gastritis in their base line biopsies showed persistence but no progression of the lesion during illness. In 4 of the volunteers who became ill, intestinal biopsies were available and showed the typical gastroenteritis lesion. These results indicate that acute infectious nonbacterial gastroenteritis induced by Norwalk agent is not associated with histologically detectable gastric mucosal lesion.", "contents": "Structure of the gastric mucosa in acute infectious bacterial gastroenteritis. It is now well documented that a characteristic mucosal lesion of the proximal small intestine is present in acute nonbacterial gastroenteritis. To determine whether a gastric mucosal lesion also accompanies this illness, stool filtrate containing Norwalk agent was given orally to 15 volunteers after base line biopsies of gastric fundal and/or antral mucosa had been obtained. Gastric fundal and/or antral biopsies were then obtained serially between 24 and 168 hr after administration of the inoculum. Nine volunteers developed symptoms of gastroenteritis. gastric biopsies from those with normal base line fundal and/or antral biopsies remained normal during and after clinical illness. Those volunteers who had mild to moderate gastritis in their base line biopsies showed persistence but no progression of the lesion during illness. In 4 of the volunteers who became ill, intestinal biopsies were available and showed the typical gastroenteritis lesion. These results indicate that acute infectious nonbacterial gastroenteritis induced by Norwalk agent is not associated with histologically detectable gastric mucosal lesion."}
{"id": "PMID:1089576", "title": "A rapid pull-through technique for measuring lower esophageal sphincter pressure.", "content": "Because disadvantages are inherent in the conventional station pull-through technique (SPT) currently used for recording lower esophageal sphincter pressure (LESP), a more suitable recording method is needed. In study, we evaluated a rapid pull-through technique (RPT) for recording LESP and compared the results with those obtained by SPT. The RPT features rapid withdrawal of recording sensors across the LES during a 10- to 20-sec interval of suspended respiration. This method avoids recording \"artifact\" caused by respiratory LES motion and provides precise end points for scoring and measurement. In 12 normal subjects, LESP measured by RPT (24.3 plus or minus 0.5 mm Hg) was comparable, but significantly greater, than LESP measured by SPT (21.1 plus or minus 9.1 mm Hg). Analysis of LESP scores for the two recording methods revealed that both intraobserver and interobserver error were substantially less for RPT than SPT (P less than or equal to 0.01). Further correlation of LESP between studies was greater for the RPT than the SPT recording method. We conclude that RPT represents a convenient method for recording LESP which is easier to perform and yields more reproducible values than LESP recording by SPT.", "contents": "A rapid pull-through technique for measuring lower esophageal sphincter pressure. Because disadvantages are inherent in the conventional station pull-through technique (SPT) currently used for recording lower esophageal sphincter pressure (LESP), a more suitable recording method is needed. In study, we evaluated a rapid pull-through technique (RPT) for recording LESP and compared the results with those obtained by SPT. The RPT features rapid withdrawal of recording sensors across the LES during a 10- to 20-sec interval of suspended respiration. This method avoids recording \"artifact\" caused by respiratory LES motion and provides precise end points for scoring and measurement. In 12 normal subjects, LESP measured by RPT (24.3 plus or minus 0.5 mm Hg) was comparable, but significantly greater, than LESP measured by SPT (21.1 plus or minus 9.1 mm Hg). Analysis of LESP scores for the two recording methods revealed that both intraobserver and interobserver error were substantially less for RPT than SPT (P less than or equal to 0.01). Further correlation of LESP between studies was greater for the RPT than the SPT recording method. We conclude that RPT represents a convenient method for recording LESP which is easier to perform and yields more reproducible values than LESP recording by SPT."}
{"id": "PMID:1089577", "title": "Effect of glucagon on meal-induced gastric secretion in man.", "content": "The effect of glucagon on gastric acid and pepsin secretion, basal or stimulated by a meal, pentagastrin and histamine, was studied in duodenal ulcer patients. Intravenous glucagon infused in graded doses ranging from 6.2 to 50 mug per kg-hr produced a dose-related inhibition of pentagastrin-induced acid secretion reaching about 40% of the control level at the dose of 50 mug per kg-hr. Acid inhibition was paralleled by a decrease in the pepsin output and serum calcium level and was accompanied by a rise in the blood glucose concentration. Glucagon used in a standard dose of 25 mug per kg-hr produced about 50% inhibition of acid secretion induced by a meal (measured by intragastric titration) accompanied by a significant decrease in the serum gastrin level measured by radioimmunoassay. Histamine-induced secretion was only slightly inhibited by glucagon, and the degree of inhibition for acid (25%) and pepsin (20%) secretion was statistically insignificant.", "contents": "Effect of glucagon on meal-induced gastric secretion in man. The effect of glucagon on gastric acid and pepsin secretion, basal or stimulated by a meal, pentagastrin and histamine, was studied in duodenal ulcer patients. Intravenous glucagon infused in graded doses ranging from 6.2 to 50 mug per kg-hr produced a dose-related inhibition of pentagastrin-induced acid secretion reaching about 40% of the control level at the dose of 50 mug per kg-hr. Acid inhibition was paralleled by a decrease in the pepsin output and serum calcium level and was accompanied by a rise in the blood glucose concentration. Glucagon used in a standard dose of 25 mug per kg-hr produced about 50% inhibition of acid secretion induced by a meal (measured by intragastric titration) accompanied by a significant decrease in the serum gastrin level measured by radioimmunoassay. Histamine-induced secretion was only slightly inhibited by glucagon, and the degree of inhibition for acid (25%) and pepsin (20%) secretion was statistically insignificant."}
{"id": "PMID:1089597", "title": "Pathogenesis of Salmonella-associated arithritis in the rat.", "content": "The distribution of joint lesions in rats with Salmonella-associated arthritis (SSA), as determined in a detailed survey, resembles to a great extent the pattern of small joint involvement in human rheumatoid arthritis. Such lesions, though regularly induced in the rat by the intravenous injection of live S.enteritidis, could not be evoked by the heat-killed organisms injected by various routes with and without extrinsic adjuvants. Efforts to transfer SAA from sensitized donors to either normal or primed recipients, employing lymphoid ce-ls from several sources, also failed repeatedly. Two observations, however, virtually exclude the possibility that joint damage in SAA can be the direct result of sustained intra-articular sepsis. First, the inoculation of as many as 10-3 viable inflammation. Second, the incidence of SAA was significantly lower in weanling rats in the adult controls although the growth and distribution of intravenously injected S. enteritidis was virtually identical in the two groups. Together these observations indicate that the joint damage occurring in SAA is determined by the host and not by the infecting organism. From this, it seems fair to conclude that the destructive arthritis characteristic of this syndrome is immunologically mediated.", "contents": "Pathogenesis of Salmonella-associated arithritis in the rat. The distribution of joint lesions in rats with Salmonella-associated arthritis (SSA), as determined in a detailed survey, resembles to a great extent the pattern of small joint involvement in human rheumatoid arthritis. Such lesions, though regularly induced in the rat by the intravenous injection of live S.enteritidis, could not be evoked by the heat-killed organisms injected by various routes with and without extrinsic adjuvants. Efforts to transfer SAA from sensitized donors to either normal or primed recipients, employing lymphoid ce-ls from several sources, also failed repeatedly. Two observations, however, virtually exclude the possibility that joint damage in SAA can be the direct result of sustained intra-articular sepsis. First, the inoculation of as many as 10-3 viable inflammation. Second, the incidence of SAA was significantly lower in weanling rats in the adult controls although the growth and distribution of intravenously injected S. enteritidis was virtually identical in the two groups. Together these observations indicate that the joint damage occurring in SAA is determined by the host and not by the infecting organism. From this, it seems fair to conclude that the destructive arthritis characteristic of this syndrome is immunologically mediated."}
{"id": "PMID:1089598", "title": "Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae.", "content": "Delipidated lysozyme digests of Nocardia opaca, N. corallina, and N. rubra have been fractionated by Sephadex filtration. The mitogenic and adjuvant activities of the fractions thus obtained have been investigated. All fractions are mitogenic except the last fraction of N.rubra, but the N. opaca products induce a stronger stimulation of mouse spleen lymphocytes than the corresponding fractions of the two other species. The activity of the first Sephadex fractions of each strain has been compared to other mitogens (concanavalin A, lipopolysaccharide). All fractions are adjuvant, although one of them, the last Sephadex fraction of N. rubra, does not contain peptidoglycan; its activity must thus be attributed to another kind of molecule. Fractionation of the first Sephadex fraction of N. opaca by centrifugation in glacial acetic acid led to a separation of adjuvant and mitogenic activities.", "contents": "Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae. Delipidated lysozyme digests of Nocardia opaca, N. corallina, and N. rubra have been fractionated by Sephadex filtration. The mitogenic and adjuvant activities of the fractions thus obtained have been investigated. All fractions are mitogenic except the last fraction of N.rubra, but the N. opaca products induce a stronger stimulation of mouse spleen lymphocytes than the corresponding fractions of the two other species. The activity of the first Sephadex fractions of each strain has been compared to other mitogens (concanavalin A, lipopolysaccharide). All fractions are adjuvant, although one of them, the last Sephadex fraction of N. rubra, does not contain peptidoglycan; its activity must thus be attributed to another kind of molecule. Fractionation of the first Sephadex fraction of N. opaca by centrifugation in glacial acetic acid led to a separation of adjuvant and mitogenic activities."}
{"id": "PMID:1089599", "title": "Deregulation of mouse antibody-forming cells in vivo in cell culture by streptococcal pyrogenic exotoxin.", "content": "An unregulated, elevated rebound of antibody levels in rabbits was shown to follow late (10 to 15 days) after steptococcal pyrogenic exotoxin (SPE)-induced immunosuppression. Because of that result we have suggested that SPE acts by preferentially inhibiting a regulatory cell which normally limits the extent of full expression of antibody formation by B-cells. We are currently testing this hypothesis in mice. NIH (Swiss Webster) mice (+/+) or NIH (Swiss Webster) mice heterozygous (+/nu) for the mutant athymic nude gene and phenotypically normal showed an elevated plaque-forming cell (PFC) response to sheep erythrocytes (SE) late (10 to 15 days) after immunosuppressive SPE treatment similar to that described in rabbits. Homozygous nude mice (nu/nu) that are phenotypically athymic normally show a reduced early (4 day) PFC response to SE (a T-cell-dependent antigen) as compared with +/nu littermates or +/+ parent strain mice. This cryptic early 4-day response was improved by injection of purified endotoxin (a B-cell mitogen), but these relatively elevated nude PFC responses had decreased to normal control (SE only)nude PFC levels before 10 days. In similar SE-injected nude mice treated instead with SPE, no elevation at 4 days was observed and, more pertinently, the late (10 to 15 day) elevated rebound of PFC levels observed in normal response controls (+/nu or +/+) was not observed. Similar experiments were subsequently conducted in Marbrook-type spleen PFC cultures during periods of 12 days. The results of these experiments paralleled the in vivo results above, and in addition showed that SPE induced a large proliferation of either +/+ or +/nu cells (T-and B-cells) in culture but had no such effect on nu/nu cells (B-cells) in culture. Purified endotoxin, the Bcell mitogen, had a better sparing effect on nu/nu cells in this respect. These results are consistent with our premise that SPE inhibits preferentially the function of a regulator of the antibody response. The regulator appears to be a T-cell and is likely a suppressor T-cell.", "contents": "Deregulation of mouse antibody-forming cells in vivo in cell culture by streptococcal pyrogenic exotoxin. An unregulated, elevated rebound of antibody levels in rabbits was shown to follow late (10 to 15 days) after steptococcal pyrogenic exotoxin (SPE)-induced immunosuppression. Because of that result we have suggested that SPE acts by preferentially inhibiting a regulatory cell which normally limits the extent of full expression of antibody formation by B-cells. We are currently testing this hypothesis in mice. NIH (Swiss Webster) mice (+/+) or NIH (Swiss Webster) mice heterozygous (+/nu) for the mutant athymic nude gene and phenotypically normal showed an elevated plaque-forming cell (PFC) response to sheep erythrocytes (SE) late (10 to 15 days) after immunosuppressive SPE treatment similar to that described in rabbits. Homozygous nude mice (nu/nu) that are phenotypically athymic normally show a reduced early (4 day) PFC response to SE (a T-cell-dependent antigen) as compared with +/nu littermates or +/+ parent strain mice. This cryptic early 4-day response was improved by injection of purified endotoxin (a B-cell mitogen), but these relatively elevated nude PFC responses had decreased to normal control (SE only)nude PFC levels before 10 days. In similar SE-injected nude mice treated instead with SPE, no elevation at 4 days was observed and, more pertinently, the late (10 to 15 day) elevated rebound of PFC levels observed in normal response controls (+/nu or +/+) was not observed. Similar experiments were subsequently conducted in Marbrook-type spleen PFC cultures during periods of 12 days. The results of these experiments paralleled the in vivo results above, and in addition showed that SPE induced a large proliferation of either +/+ or +/nu cells (T-and B-cells) in culture but had no such effect on nu/nu cells (B-cells) in culture. Purified endotoxin, the Bcell mitogen, had a better sparing effect on nu/nu cells in this respect. These results are consistent with our premise that SPE inhibits preferentially the function of a regulator of the antibody response. The regulator appears to be a T-cell and is likely a suppressor T-cell."}
{"id": "PMID:1089600", "title": "Immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms.", "content": "Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous bacteria. PFC reacting with these bacterial antigens were not detected in infant SPF mice until 7 days after birth. Compared with nonvaccinated controls, SPF mice vaccinated parenterally with indigenous E. coli or Bacteroides produced a moderate increase in the numbers of specific PFC. Thus, the SPF mouse is capable of responding immunologically after vaccination with microbes indigenous to its intestinal tract. However, more PFC reacting with homologous vaccine antigens were detected after parenteral vaccination of SPF mice with nonindigenous E. coli O127:B8, E.coli O14, or B. fragilis than after parenteral vaccination with indigenous E. coli or Bacteroides. Gnotobiotic mice orally monoassociated with these nonindigenous bacteria exhibited greater immune responses to antigens of the bacteria used to monoassociation than did gnotobiotes monoassociated with the indigenous microbes. The results are consistent with the hypothesis that mice are more responsive immunologically to antigens of nonindigenous bacteria than they are to antigens of certain microbes indigenous to their gastrointestinal tracts.", "contents": "Immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms. Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous bacteria. PFC reacting with these bacterial antigens were not detected in infant SPF mice until 7 days after birth. Compared with nonvaccinated controls, SPF mice vaccinated parenterally with indigenous E. coli or Bacteroides produced a moderate increase in the numbers of specific PFC. Thus, the SPF mouse is capable of responding immunologically after vaccination with microbes indigenous to its intestinal tract. However, more PFC reacting with homologous vaccine antigens were detected after parenteral vaccination of SPF mice with nonindigenous E. coli O127:B8, E.coli O14, or B. fragilis than after parenteral vaccination with indigenous E. coli or Bacteroides. Gnotobiotic mice orally monoassociated with these nonindigenous bacteria exhibited greater immune responses to antigens of the bacteria used to monoassociation than did gnotobiotes monoassociated with the indigenous microbes. The results are consistent with the hypothesis that mice are more responsive immunologically to antigens of nonindigenous bacteria than they are to antigens of certain microbes indigenous to their gastrointestinal tracts."}
{"id": "PMID:1089601", "title": "Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.", "content": "A rapid, potentially clinically useful test for detection of enterotoxigenic Escherichia coli is described. Whole bacterial cultures of enterotoxigenic E. coli, when briefly exposed to Y1 adrenal cells in tissue miniculture, effect a rounding response in the tissue culture that can be discerned at 18 to 24 h. The tissue culture technique agreed with the rabbit ileal loop in all 58 enterotoxigenic and 52 non-enterotoxigenic E. coli strains tested.", "contents": "Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. A rapid, potentially clinically useful test for detection of enterotoxigenic Escherichia coli is described. Whole bacterial cultures of enterotoxigenic E. coli, when briefly exposed to Y1 adrenal cells in tissue miniculture, effect a rounding response in the tissue culture that can be discerned at 18 to 24 h. The tissue culture technique agreed with the rabbit ileal loop in all 58 enterotoxigenic and 52 non-enterotoxigenic E. coli strains tested."}
{"id": "PMID:1089602", "title": "In vitro interaction of Bacteroides fragilis with polymorphonuclear leukocytes and serum factors.", "content": "The sera and polymorphonuclear leukocytes (PMNs) of healthy human subjects were tested against several isolates of Bacteroides fragilis. All sera killed most of the fecal isolates, but were active aganist only one of the clinical isolates. The degree of killing was directly related to the serum concentration but was independent of the bacterial inoculum within the range of bacterial concentrations studied. The serum bactericidal activity was heat-labile. Engulfment and killing of B. fragilis by PMNs were demonstrated consistently.  The opsonim involved in phagocytosis was also heat liable. Calculations involving log-transformed data permitted the quantitative study of the separate and combined effects of serum and PMNs on bacteria which were killed by serum alone. Whereas serum alone usually killed 0.5 log of serum-sensitive bacteria, the addition of PMNs was usually associated with one log further killing. The studies reported here demonstrate the presence of heat-labile serum factors in normal human sera which killed B. fragilis directly and which promote its phagocytosis and killing by PMNs. These observations provide a foundation for investigations into host defense mechanisms against anaerobes.", "contents": "In vitro interaction of Bacteroides fragilis with polymorphonuclear leukocytes and serum factors. The sera and polymorphonuclear leukocytes (PMNs) of healthy human subjects were tested against several isolates of Bacteroides fragilis. All sera killed most of the fecal isolates, but were active aganist only one of the clinical isolates. The degree of killing was directly related to the serum concentration but was independent of the bacterial inoculum within the range of bacterial concentrations studied. The serum bactericidal activity was heat-labile. Engulfment and killing of B. fragilis by PMNs were demonstrated consistently.  The opsonim involved in phagocytosis was also heat liable. Calculations involving log-transformed data permitted the quantitative study of the separate and combined effects of serum and PMNs on bacteria which were killed by serum alone. Whereas serum alone usually killed 0.5 log of serum-sensitive bacteria, the addition of PMNs was usually associated with one log further killing. The studies reported here demonstrate the presence of heat-labile serum factors in normal human sera which killed B. fragilis directly and which promote its phagocytosis and killing by PMNs. These observations provide a foundation for investigations into host defense mechanisms against anaerobes."}
{"id": "PMID:1089603", "title": "Association of Salmonella typhimurium with, and its invasion of, the ileal mucosa in mice.", "content": "A wild-type strain of Salmonella typhimurium and three mutant rough colonial variants of the wild type were compared for their ability to become associated with and invade the ileal mucosa of germfree and specific-pathogen-free mice. The rough-mutant strains differed from the wild type in having incomplete lipopolysaccharides lacking one or more sugars in the polysaccharide moiety. The wild-type and mutant strains also differed one from the other in the types of appendages (flagella, pili) on their surfaces. Depending upon the dosage of bacteria given, all mutant strains as well as the wild type could associate with and invade the intestinal mucosa of infected gnotobiotic mice. If the infecting dosage was high enough, at least two of the mutant strains and the wild type invade the intestinal mucosa of the specific-pathogen-free animals. O antigen, flagella, or pili do not appear to be essential for the association of S. typhimurium with the mucosal surface of the mouse ileum. O antigen on the bacterial cell surface may be important, but not essential, for invasion of the ileal mucosa.", "contents": "Association of Salmonella typhimurium with, and its invasion of, the ileal mucosa in mice. A wild-type strain of Salmonella typhimurium and three mutant rough colonial variants of the wild type were compared for their ability to become associated with and invade the ileal mucosa of germfree and specific-pathogen-free mice. The rough-mutant strains differed from the wild type in having incomplete lipopolysaccharides lacking one or more sugars in the polysaccharide moiety. The wild-type and mutant strains also differed one from the other in the types of appendages (flagella, pili) on their surfaces. Depending upon the dosage of bacteria given, all mutant strains as well as the wild type could associate with and invade the intestinal mucosa of infected gnotobiotic mice. If the infecting dosage was high enough, at least two of the mutant strains and the wild type invade the intestinal mucosa of the specific-pathogen-free animals. O antigen, flagella, or pili do not appear to be essential for the association of S. typhimurium with the mucosal surface of the mouse ileum. O antigen on the bacterial cell surface may be important, but not essential, for invasion of the ileal mucosa."}
{"id": "PMID:1089604", "title": "Phagocytosis of Mycoplasma salivarium by human polymorphonuclear leukocytes and monocytes.", "content": "Mycoplasmacial activity was exhibited by human peripheal blood leukocytes in the absence of detectable specific antiserum. After incubation of varying concentrations of Mycoplasma salivarium with leukocytes, changes in colony-forming units (CFU) of this species per millilter occurred. The most noticeable decrease in CFU per milliliter was then the incubation mixtures contained five mycoplasmas per leukocyte. At this ratio, the mycoplasmacial min of incubation. Continued incubation demonstrated a tenfold decrease in CFU per milliliter by 4 h. Electron micrographs of incubated mixtures of human leukocytes and M. salivarium showed this mycoplasma to be phagocytized by monocytes and neutrophils whenever mutual contact or pseudopodial formation occurred. The process was continuous. Numerous phagocytic vacuoles developed which contained multiple ingested microorganisms. After the cytoplasmic granules of the leukocytes fused with the phagocytic vacuole, the phagocytized mycoplasmas became disrupted and unrecognizable.", "contents": "Phagocytosis of Mycoplasma salivarium by human polymorphonuclear leukocytes and monocytes. Mycoplasmacial activity was exhibited by human peripheal blood leukocytes in the absence of detectable specific antiserum. After incubation of varying concentrations of Mycoplasma salivarium with leukocytes, changes in colony-forming units (CFU) of this species per millilter occurred. The most noticeable decrease in CFU per milliliter was then the incubation mixtures contained five mycoplasmas per leukocyte. At this ratio, the mycoplasmacial min of incubation. Continued incubation demonstrated a tenfold decrease in CFU per milliliter by 4 h. Electron micrographs of incubated mixtures of human leukocytes and M. salivarium showed this mycoplasma to be phagocytized by monocytes and neutrophils whenever mutual contact or pseudopodial formation occurred. The process was continuous. Numerous phagocytic vacuoles developed which contained multiple ingested microorganisms. After the cytoplasmic granules of the leukocytes fused with the phagocytic vacuole, the phagocytized mycoplasmas became disrupted and unrecognizable."}
{"id": "PMID:1089605", "title": "Effect of local corticosteroids on antibody-forming cells in the eye and draining lymph nodes.", "content": "Significant numbers of antibody-forming cells (AFC) have been found in the cornea, uveal tract, and draining lymph nodes after the intracorneal injection of bovine gamma-globulin (BGG). To study the effect of locally administered corticosteroids on these antibody-forming tissues, we made unilateral intracorneal injections of rabbit eyes with BGG. These we followed immediately with subconjunctival injections of 10 mg. of triamcinolone suspension, and then with a second round of 10 mg. injections seven days later. A control group of animals received the BGG injections followed by two subconjunctival saline injections. We killed the animals on postinjection days 6, 9, 12, 15, and 21, and tested the draining lymph nodes, homolateral uveal tissue, and homolateral cornea for AFC by a modification of the Jerne placque technique. The local steroids had no effect on the number of AFC produced in the draining lymph nodes or on the circulating antibody response, but they reduced the number of AFC in the homolateral uveal tracts and corneas. Clinically there was less inflammatory response in the steroid-treated eyes than in the control eyes. The possible mechanisms by which corticosteroids achieve their anti-immunologic and anti-inflammatory benefits are discussed.", "contents": "Effect of local corticosteroids on antibody-forming cells in the eye and draining lymph nodes. Significant numbers of antibody-forming cells (AFC) have been found in the cornea, uveal tract, and draining lymph nodes after the intracorneal injection of bovine gamma-globulin (BGG). To study the effect of locally administered corticosteroids on these antibody-forming tissues, we made unilateral intracorneal injections of rabbit eyes with BGG. These we followed immediately with subconjunctival injections of 10 mg. of triamcinolone suspension, and then with a second round of 10 mg. injections seven days later. A control group of animals received the BGG injections followed by two subconjunctival saline injections. We killed the animals on postinjection days 6, 9, 12, 15, and 21, and tested the draining lymph nodes, homolateral uveal tissue, and homolateral cornea for AFC by a modification of the Jerne placque technique. The local steroids had no effect on the number of AFC produced in the draining lymph nodes or on the circulating antibody response, but they reduced the number of AFC in the homolateral uveal tracts and corneas. Clinically there was less inflammatory response in the steroid-treated eyes than in the control eyes. The possible mechanisms by which corticosteroids achieve their anti-immunologic and anti-inflammatory benefits are discussed."}
{"id": "PMID:1089606", "title": "Fracture faces of fenestrations and junctions of endothelial cells in human choroidal vessels.", "content": "Replicas of freeze-fractured endothelial cells of human choroidal capillaries show fenestrations with a central thickening, as described in thin-section studies. Between the endothelial cells there are discontinuous zonulae occludentes (i.e., maculae or fasciae occludentes), composed of ridges on face A and grooves on face B of the split-cell membrane. They are also present along the sutures of the endothelial cells of venules. Close to the intercellular suture gap junctions, probably connecting capillary endothelial cells and pericytes, are found.", "contents": "Fracture faces of fenestrations and junctions of endothelial cells in human choroidal vessels. Replicas of freeze-fractured endothelial cells of human choroidal capillaries show fenestrations with a central thickening, as described in thin-section studies. Between the endothelial cells there are discontinuous zonulae occludentes (i.e., maculae or fasciae occludentes), composed of ridges on face A and grooves on face B of the split-cell membrane. They are also present along the sutures of the endothelial cells of venules. Close to the intercellular suture gap junctions, probably connecting capillary endothelial cells and pericytes, are found."}
{"id": "PMID:1089607", "title": "Sutures and bladder wound healing in the experimental animal.", "content": "An incision was made in the bladder of each of 81 female rats, then closed with various types and sizes of sutures and by different methods in order to determine the optimum suture and method to facilitate watertight closure and wound healing. Chromic catgut, collagen, and polyglycolic acid sutures, of different sizes, were used. Pressure studies and microscopic examinations of the wound were done at different intervals. All sutures lasted long enough to allow healing of the wound and none broke during pressure studies. A two-layer continuous closure with fine suture was the most watertight. Tissue reaction was almost absent when polyglycolic acid suture was used and was minimal with fine chromic catgut.", "contents": "Sutures and bladder wound healing in the experimental animal. An incision was made in the bladder of each of 81 female rats, then closed with various types and sizes of sutures and by different methods in order to determine the optimum suture and method to facilitate watertight closure and wound healing. Chromic catgut, collagen, and polyglycolic acid sutures, of different sizes, were used. Pressure studies and microscopic examinations of the wound were done at different intervals. All sutures lasted long enough to allow healing of the wound and none broke during pressure studies. A two-layer continuous closure with fine suture was the most watertight. Tissue reaction was almost absent when polyglycolic acid suture was used and was minimal with fine chromic catgut."}
{"id": "PMID:1089609", "title": "Cutaneous everted nipple ureterostomy.", "content": "Normal dog ureter transplanted to the skin by the everted nipple ureterostomy technique was found to be far superior to the skin flap ureterostomy in nevovascularization and healing of the stoma. The results suggest a possibility of successful application in the human; recent clinical experience supports animal data.", "contents": "Cutaneous everted nipple ureterostomy. Normal dog ureter transplanted to the skin by the everted nipple ureterostomy technique was found to be far superior to the skin flap ureterostomy in nevovascularization and healing of the stoma. The results suggest a possibility of successful application in the human; recent clinical experience supports animal data."}
{"id": "PMID:1089611", "title": "Experimental lipid A-induced nephritis in the dog.", "content": "The effect of radioactive lipid A, obtained from Escherichia coli, on the kidney of adult dogs and puppies was investigated. Injection of lipid A into the temporarily occluded renal pelvis of adult dogs caused abacterial interstitial nephritis in all animals tested. The intensity and duration of the kidney response coorelated well with the lipid A dose administered. Lipid A was demonstrated autoradiographically in the renal cortex, extra- and intracellularly. Radioactivity was still present after 10 weeks in 16 of 20 examined dogs. In the remaining four dogs the interstitial nephritis had subsided. Thirteen of 14 puppies in which the immunologic defense mechanisms had not yet developed showed no histologic reaction in the kidney after the same procedure as in the adult dogs. Lipid A appeared in the renal parenchyma through the blood stream rather than through the retrograde route. Lipid A antibody titers could be detected only in adult dogs, never in puppies. Lipid A apparently provoked an immunologic process in the kidney damaged by the operative manipulations, thus resulting in an abacterial interstitial nephritis. The possibility that chronic abacterial pyelonephritis can be induced clinically through lipid A and thus may have a pathogenesis similar to abacterial interstitial nephritis is discussed.", "contents": "Experimental lipid A-induced nephritis in the dog. The effect of radioactive lipid A, obtained from Escherichia coli, on the kidney of adult dogs and puppies was investigated. Injection of lipid A into the temporarily occluded renal pelvis of adult dogs caused abacterial interstitial nephritis in all animals tested. The intensity and duration of the kidney response coorelated well with the lipid A dose administered. Lipid A was demonstrated autoradiographically in the renal cortex, extra- and intracellularly. Radioactivity was still present after 10 weeks in 16 of 20 examined dogs. In the remaining four dogs the interstitial nephritis had subsided. Thirteen of 14 puppies in which the immunologic defense mechanisms had not yet developed showed no histologic reaction in the kidney after the same procedure as in the adult dogs. Lipid A appeared in the renal parenchyma through the blood stream rather than through the retrograde route. Lipid A antibody titers could be detected only in adult dogs, never in puppies. Lipid A apparently provoked an immunologic process in the kidney damaged by the operative manipulations, thus resulting in an abacterial interstitial nephritis. The possibility that chronic abacterial pyelonephritis can be induced clinically through lipid A and thus may have a pathogenesis similar to abacterial interstitial nephritis is discussed."}
{"id": "PMID:1089612", "title": "Experimental pyelonephritis in the monkey. II. The prognostic value of radionuclide evaluation of the urinary tract.", "content": "The radionuclide scintiphoto study, a noninvasive technique, is abnormal in acute pyelonephritis. A delay in excretion of the isotope has also been observed in dehydrated studies in chronic pyelonephritis. To further elucidate this abnormal pattern in pyelonephritis, experimental renal infection was produced in monkeys. Dehydrated 131-I-hippuran scintiphoto studies were conducted weekly for 8 weeks after the initial infection. The scintiphoto studies showed significant delay in excretion of radionuclide in all bacteriuric monkeys at 1 week. After this time, delayed excretion was seen in only those monkeys later classified as having chronic pyelonephritis. This was statistically significant (P smaller than 0.01).", "contents": "Experimental pyelonephritis in the monkey. II. The prognostic value of radionuclide evaluation of the urinary tract. The radionuclide scintiphoto study, a noninvasive technique, is abnormal in acute pyelonephritis. A delay in excretion of the isotope has also been observed in dehydrated studies in chronic pyelonephritis. To further elucidate this abnormal pattern in pyelonephritis, experimental renal infection was produced in monkeys. Dehydrated 131-I-hippuran scintiphoto studies were conducted weekly for 8 weeks after the initial infection. The scintiphoto studies showed significant delay in excretion of radionuclide in all bacteriuric monkeys at 1 week. After this time, delayed excretion was seen in only those monkeys later classified as having chronic pyelonephritis. This was statistically significant (P smaller than 0.01)."}
{"id": "PMID:1089613", "title": "Benurestat, a urease inhibitor for the therapy of infected ureolysis.", "content": "A single oral administration of the urease inhibitor benurestat (2-(p-chlorobenz-amido)acetohydroxamic acid) to the human at 15 or 25 mg per kg produced, for 4 hr, mean urinary levels of inhibitory activity that were 700 to 1900 times that equivalent concentration of benurestat required to inhibit Proteus mirabilis urease by 90 per cent. In the rat these same dosage levels produced urinary inhibitory activity equivalent to 16 to 140 fold that required for 90 per cent urease inhibition. Benurestat administration, 25, 50, or 100 mg per kg, caused a decrease in the urinary excretion of ammonia from rats with experimental P. mirabilis genitourinary tract infection. The formation of struvite calculi was inhibited under these conditions. Nitrofurantoin, sulfamethoxazole, and ampicillin also slowed the formation of struvite calculi in infected rats and together with benurestat a potentiation of the inhibition of calculi formation was secured. Some combination therapies composed of benurestat plus an antibacterial agent, sulfamethoxazole or ampicillin, were effective in promoting the net dissolution of formed calculi. The number of viable bacteria present in the bladders of infected rats was significantly less after the administration of benurestat plus nitrofurantoin, sulfamethoxazole, or ampicillin than the respective numbers that were obtained from control infected rats or from rats administered either component of the combination separately.", "contents": "Benurestat, a urease inhibitor for the therapy of infected ureolysis. A single oral administration of the urease inhibitor benurestat (2-(p-chlorobenz-amido)acetohydroxamic acid) to the human at 15 or 25 mg per kg produced, for 4 hr, mean urinary levels of inhibitory activity that were 700 to 1900 times that equivalent concentration of benurestat required to inhibit Proteus mirabilis urease by 90 per cent. In the rat these same dosage levels produced urinary inhibitory activity equivalent to 16 to 140 fold that required for 90 per cent urease inhibition. Benurestat administration, 25, 50, or 100 mg per kg, caused a decrease in the urinary excretion of ammonia from rats with experimental P. mirabilis genitourinary tract infection. The formation of struvite calculi was inhibited under these conditions. Nitrofurantoin, sulfamethoxazole, and ampicillin also slowed the formation of struvite calculi in infected rats and together with benurestat a potentiation of the inhibition of calculi formation was secured. Some combination therapies composed of benurestat plus an antibacterial agent, sulfamethoxazole or ampicillin, were effective in promoting the net dissolution of formed calculi. The number of viable bacteria present in the bladders of infected rats was significantly less after the administration of benurestat plus nitrofurantoin, sulfamethoxazole, or ampicillin than the respective numbers that were obtained from control infected rats or from rats administered either component of the combination separately."}
{"id": "PMID:1089614", "title": "Succinic dehydrogenase as a measure of tissue viability in the cadaver kidney. Influence of perfusing solutions.", "content": "Succinic dehydrogenase (SDHG) has been studied in the rabbit cadaver kidney. Increasing warm ischemia time decreases the ability of the organ to utilize oxygen and therefore lowers its SDHG activity. The addition of perfusing solutions further decreases the SDHG activity; saline has a more adverse affect than either Perfudex or albumin.", "contents": "Succinic dehydrogenase as a measure of tissue viability in the cadaver kidney. Influence of perfusing solutions. Succinic dehydrogenase (SDHG) has been studied in the rabbit cadaver kidney. Increasing warm ischemia time decreases the ability of the organ to utilize oxygen and therefore lowers its SDHG activity. The addition of perfusing solutions further decreases the SDHG activity; saline has a more adverse affect than either Perfudex or albumin."}
{"id": "PMID:1089620", "title": "Canine granulocytopathy syndrome: neutrophil dysfunction in a dog with recurrent infections.", "content": "A male Irish Setter dog had a clinical history of recurrent life-threatening bacterial infections, with associated periods of pyrexia and severe neutrophilia. Examination of a mandibular lymph node biopsy made when the patient was 10 weeks old revealed subacute diffuse suppurative lymphadenitis with reticuloendothelial hyperplasia. Circulating leukocytes isolated from the dog when it was 5 months old had a marked bactericidal defect when compared with cells from clinically normal dogs of the same age. The clinical syndrome in the affected patient resembled that observed in the granulocytopathies described in man and other animals.", "contents": "Canine granulocytopathy syndrome: neutrophil dysfunction in a dog with recurrent infections. A male Irish Setter dog had a clinical history of recurrent life-threatening bacterial infections, with associated periods of pyrexia and severe neutrophilia. Examination of a mandibular lymph node biopsy made when the patient was 10 weeks old revealed subacute diffuse suppurative lymphadenitis with reticuloendothelial hyperplasia. Circulating leukocytes isolated from the dog when it was 5 months old had a marked bactericidal defect when compared with cells from clinically normal dogs of the same age. The clinical syndrome in the affected patient resembled that observed in the granulocytopathies described in man and other animals."}
{"id": "PMID:1089617", "title": "Stanford cardiac transplantation. A review.", "content": "The cardiac transplantation experience at Stanford University School of Medicine commenced in 1959 with the development of a comprehensive surgical method for successful orthotopic cardiac transplantation in experimental animals. The steps involved included atrial level transection and anastomosis, local myocardial hypothermia and cardiopulmonary bypass. From the first Stanford clinical case in January 1968 until May 1974, 73 transplants have been carried out in 71 patients. Cardiac transplantation is indicated in myopathic disease of an advanced nature with the prospect of imminent death. Contraindications are high pulmonary vascular resistance and intercurrent infection. Relative contraindications are advanced age and insulin-dependent diabetes. Previous cardiac surgery procedures are not contraindications to transplantation. Maintenance immunosuppression requires azathioprine and prednisone indefinitely. Antithymocyte globulin is used for a short time in the postoperative period. Anticoagulants and antiplatelet agents are also used. Therapy for rejection crises includes methylprednisolone, in gram amounts, and actinomycin D. The diagnosis of graft rejection is based on a decrease in electrocardiographic voltage, transvenous endomyocardial biopsy and clinical examination of the patient. The overall survival rate is 43% at one year and 39% at two years. The longest-living survivor in the series is doing a regular day's work five years after transplantation.", "contents": "Stanford cardiac transplantation. A review. The cardiac transplantation experience at Stanford University School of Medicine commenced in 1959 with the development of a comprehensive surgical method for successful orthotopic cardiac transplantation in experimental animals. The steps involved included atrial level transection and anastomosis, local myocardial hypothermia and cardiopulmonary bypass. From the first Stanford clinical case in January 1968 until May 1974, 73 transplants have been carried out in 71 patients. Cardiac transplantation is indicated in myopathic disease of an advanced nature with the prospect of imminent death. Contraindications are high pulmonary vascular resistance and intercurrent infection. Relative contraindications are advanced age and insulin-dependent diabetes. Previous cardiac surgery procedures are not contraindications to transplantation. Maintenance immunosuppression requires azathioprine and prednisone indefinitely. Antithymocyte globulin is used for a short time in the postoperative period. Anticoagulants and antiplatelet agents are also used. Therapy for rejection crises includes methylprednisolone, in gram amounts, and actinomycin D. The diagnosis of graft rejection is based on a decrease in electrocardiographic voltage, transvenous endomyocardial biopsy and clinical examination of the patient. The overall survival rate is 43% at one year and 39% at two years. The longest-living survivor in the series is doing a regular day's work five years after transplantation."}
{"id": "PMID:1089618", "title": "The modified bovine carotid artery as a vascular substitute in man. Personal experience and review of the literature.", "content": "The modified bovine heterograft is one of the vascular prostheses currently available for clinical use. This report reviews the mode of preparation of the heterograft and its physical and biological qualities. The experience of the authors with this heterograft for vascular reconstruction in 32 patients and for the construction of s.c. arteriovenous (A-V) fistulas in 80 patients is reported. The modified bovine heterograft was found to be a satisfactory prosthesis when used in vascular reconstruction above the inguinal ligament or for the construction of A-V fistulas for hemodialysis. In reconstructions in the femoropopliteal segment of the circulation, the use of the heterograft by the authors was associated with an unacceptable rate of morbidity and graft failures. Experience reported in the literature has so far been incomplete and inconclusive, with one-year patency rates ranging between 20 and 74%.", "contents": "The modified bovine carotid artery as a vascular substitute in man. Personal experience and review of the literature. The modified bovine heterograft is one of the vascular prostheses currently available for clinical use. This report reviews the mode of preparation of the heterograft and its physical and biological qualities. The experience of the authors with this heterograft for vascular reconstruction in 32 patients and for the construction of s.c. arteriovenous (A-V) fistulas in 80 patients is reported. The modified bovine heterograft was found to be a satisfactory prosthesis when used in vascular reconstruction above the inguinal ligament or for the construction of A-V fistulas for hemodialysis. In reconstructions in the femoropopliteal segment of the circulation, the use of the heterograft by the authors was associated with an unacceptable rate of morbidity and graft failures. Experience reported in the literature has so far been incomplete and inconclusive, with one-year patency rates ranging between 20 and 74%."}
{"id": "PMID:1089622", "title": "Dimensions of the pet population problem.", "content": "In 1973, an estimated $4.5 billion was spent on the combined care and feeding of pets, whereas about 18 million neglected pets were impounded, costing our society nearly $125 million. About 72,000 dogs and cats must die or be killed each day to maintain zero growth in the population. Humane shelters and pounds have become slaughterhouses. Neglected pets constitute a hazard to the health and quality of life of man and other animals. Sizable segments of society are becomming hostile toward dogs and cats.", "contents": "Dimensions of the pet population problem. In 1973, an estimated $4.5 billion was spent on the combined care and feeding of pets, whereas about 18 million neglected pets were impounded, costing our society nearly $125 million. About 72,000 dogs and cats must die or be killed each day to maintain zero growth in the population. Humane shelters and pounds have become slaughterhouses. Neglected pets constitute a hazard to the health and quality of life of man and other animals. Sizable segments of society are becomming hostile toward dogs and cats."}
{"id": "PMID:1089623", "title": "New azasteroidal antifungal antibiotics from Geotrichum flavo-brunneum. III. Biological activity.", "content": "The A25822 antibiotic complex consists of seven biologically active factors. A comparative study of these factors determined that factor B possessed the greatest antifungal activity. The minimal inhibitory concentration of A25822B against isolates of Candida albicans was less than 0.3 similar to 5.0 mug/ml, Trichophyton mentagrophytes was inhibited at less than 0.0312 mug/ml. Other pathogenic fungi such as Cryptococcus neoformans, Histoplasma capsulatum, Blastomyces dermatitidis, Sporotrichum schenckii, and Microsporium gypseum were very susceptible to A25822B. Only limited antibacterial activity of A25822B was found. Parenteral or oral administration of 50 mg/kg of A25822B significantly extended the average survival time of mice infected with C. albicans. Doses of 20 mg/kg of A25822B caused a greater than ten-fold reduction in the number of Candida cells recovered from kidneys of infected mice. A solution of 0.5% or 0.25% A25822B applied topically was effective against an experimental dermatophyte infection on guinea pigs. A peak blood level of 3 mug/ml was achieved in mice following a 100 mg/kg dose of A25822B. Combination of A25822B with a polyene antibiotic in vitro showed antagonism.", "contents": "New azasteroidal antifungal antibiotics from Geotrichum flavo-brunneum. III. Biological activity. The A25822 antibiotic complex consists of seven biologically active factors. A comparative study of these factors determined that factor B possessed the greatest antifungal activity. The minimal inhibitory concentration of A25822B against isolates of Candida albicans was less than 0.3 similar to 5.0 mug/ml, Trichophyton mentagrophytes was inhibited at less than 0.0312 mug/ml. Other pathogenic fungi such as Cryptococcus neoformans, Histoplasma capsulatum, Blastomyces dermatitidis, Sporotrichum schenckii, and Microsporium gypseum were very susceptible to A25822B. Only limited antibacterial activity of A25822B was found. Parenteral or oral administration of 50 mg/kg of A25822B significantly extended the average survival time of mice infected with C. albicans. Doses of 20 mg/kg of A25822B caused a greater than ten-fold reduction in the number of Candida cells recovered from kidneys of infected mice. A solution of 0.5% or 0.25% A25822B applied topically was effective against an experimental dermatophyte infection on guinea pigs. A peak blood level of 3 mug/ml was achieved in mice following a 100 mg/kg dose of A25822B. Combination of A25822B with a polyene antibiotic in vitro showed antagonism."}
{"id": "PMID:1089625", "title": "New azasteroidal antifungal antibotics from Geotrichum flavo-brunneum. I. Discovery and fermentation studies.", "content": "Although Geotrichum species occur ubiquitously, antibiotic production by members of this genus has not previously been reported. The antibiotic complex designated A25822, consisting of one major and six minor structurally-related components active primarily against Candida and Trichophyton, represents a new family of naturally-occurring compounds. Approximately 90% of the antibiotic activity synthesized remained associated with the fungal cell mass, from which it was recovered by multiple methanolic extractions for quantitation. Antibiotic production was enhanced by tryptophan, iron, zinc, and high levels of dextrin.", "contents": "New azasteroidal antifungal antibotics from Geotrichum flavo-brunneum. I. Discovery and fermentation studies. Although Geotrichum species occur ubiquitously, antibiotic production by members of this genus has not previously been reported. The antibiotic complex designated A25822, consisting of one major and six minor structurally-related components active primarily against Candida and Trichophyton, represents a new family of naturally-occurring compounds. Approximately 90% of the antibiotic activity synthesized remained associated with the fungal cell mass, from which it was recovered by multiple methanolic extractions for quantitation. Antibiotic production was enhanced by tryptophan, iron, zinc, and high levels of dextrin."}
{"id": "PMID:1089626", "title": "Uptake of adenosine 5'-monophosphate by Escherichia coli.", "content": "Adenosine 5'-monophosphate is dephosphorylated before its uptake by cells of Escherichia coli. This is demonstrated by using a radioactive double-labeled culture, and with a 5'-nucleotidase-deficient, mutant strain. The adenosine formed is further phosphorolyzed to adenine as a prerequisite for its uptake and incorporation. The cellular localization of the enzymes involved in the catabolism of adenosine 5'-monophosphate is discussed.", "contents": "Uptake of adenosine 5'-monophosphate by Escherichia coli. Adenosine 5'-monophosphate is dephosphorylated before its uptake by cells of Escherichia coli. This is demonstrated by using a radioactive double-labeled culture, and with a 5'-nucleotidase-deficient, mutant strain. The adenosine formed is further phosphorolyzed to adenine as a prerequisite for its uptake and incorporation. The cellular localization of the enzymes involved in the catabolism of adenosine 5'-monophosphate is discussed."}
{"id": "PMID:1089627", "title": "Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate.", "content": "The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fraction of ribosomes actively engaged in translation was estimated by sucrose gradient centrifugation of ribosomes and polysomes. These data were used in a calculation of the apparent time taken for the addition of an amino acid to the growing polypeptide chain; this value was found to vary linearly with growth rate over a fivefold range of doubling times.", "contents": "Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate. The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fraction of ribosomes actively engaged in translation was estimated by sucrose gradient centrifugation of ribosomes and polysomes. These data were used in a calculation of the apparent time taken for the addition of an amino acid to the growing polypeptide chain; this value was found to vary linearly with growth rate over a fivefold range of doubling times."}
{"id": "PMID:1089628", "title": "Characterization of lipopolysaccharides from Escherichia coli K-12 mutants.", "content": "Chemical analyses of the carbohydrate composition of lipopolysaccharides (LPS) from a number of LPS mutants were used to propose a schematic composition for the LPS from Escherichia coli K-12. The formula contains four regions: the first consists of lipid A, ketodeoxyoctonoic acid, and a phosphorous component; the second contains only heptose; the third only glucose; and the fourth additional glucose, galactose, and rhamnose. LPS from E. coli B may have a similar composition but lacks the galactose and rhamnose units. A set of LPS-specific bacteriophages were used for comparing three mutants of Salmonella with a number of LPS mutants of E. coli K-12. The results confirm that there are basic similarities in the first and second regions of the LPS structure; they also support the four region divisions of the LPS formula. Paper chromatography was used for characterization of 32-P-labeled LPS from different strains of E. coli and Salmonella. The Rf values for LPS varied from 0.27 to 0.75 depending on the amounts of carbohydrates in the molecule. LPS from all strains studied was homogenous except for strain D31 which produced two types of LPS. Mild acid hydrolysis of labeled LPS liberated lipid A and two other components with phosphate, one of which was assigned to the first region. It is suggested that paper chromatography can be used in biosynthetic studies concerning regions 2 to 4.", "contents": "Characterization of lipopolysaccharides from Escherichia coli K-12 mutants. Chemical analyses of the carbohydrate composition of lipopolysaccharides (LPS) from a number of LPS mutants were used to propose a schematic composition for the LPS from Escherichia coli K-12. The formula contains four regions: the first consists of lipid A, ketodeoxyoctonoic acid, and a phosphorous component; the second contains only heptose; the third only glucose; and the fourth additional glucose, galactose, and rhamnose. LPS from E. coli B may have a similar composition but lacks the galactose and rhamnose units. A set of LPS-specific bacteriophages were used for comparing three mutants of Salmonella with a number of LPS mutants of E. coli K-12. The results confirm that there are basic similarities in the first and second regions of the LPS structure; they also support the four region divisions of the LPS formula. Paper chromatography was used for characterization of 32-P-labeled LPS from different strains of E. coli and Salmonella. The Rf values for LPS varied from 0.27 to 0.75 depending on the amounts of carbohydrates in the molecule. LPS from all strains studied was homogenous except for strain D31 which produced two types of LPS. Mild acid hydrolysis of labeled LPS liberated lipid A and two other components with phosphate, one of which was assigned to the first region. It is suggested that paper chromatography can be used in biosynthetic studies concerning regions 2 to 4."}
{"id": "PMID:1089629", "title": "Blocking of bacteriophages phi W and phi 5 with lipopolysaccharides from Escherichia coli K-12 mutants.", "content": "In the preceding paper we presented a formula for the composition of lipopolysaccharides (LPS) from Escherichia coli K-12. This formula contains four regions defined from analyses of LPS from four key strains, the parent and mutants which had lost one, two, or three regions of their carbohydrates. Support for the formula was derived from the susceptibility of the key mutants to several bacteriophages. One of these, phage phi W, was found specific for strains which had lost region 4. In this paper we described inactivation in vitro of phage phi W and its host-range mutant phi 5, using LPS devoid of regions 2 to 4. The blocking of phi W was found to require about 0.15 M concentrations of monovalent cations and to be inhibited by low concentrations of calcium and magnesium ions. One particle of phage phi W required 2 times 10-16 g of LPS devoid of region 4 for stoichiometric inactivation. Phage phi 5 was blocked by both heptose-less LPS (devoid of regions 2 to 4) and glucose-less LPS (devoid of regions 3 to 4) but was unaffected by LPS devoid of region 4. LPS from a heptose-less mutant of Salmonella minnesota showed the same inactivation ability as did LPS from heptose-less strains of E. coli K-12. Lipid A was prepared from LPS containing all four regions. Such lipid A was found to inactivate phi 5, whereas both the polysaccharide moiety as well as the intact LPS were without effect. It is suggested that lipid A is part of the receptor site for phage phi 5.", "contents": "Blocking of bacteriophages phi W and phi 5 with lipopolysaccharides from Escherichia coli K-12 mutants. In the preceding paper we presented a formula for the composition of lipopolysaccharides (LPS) from Escherichia coli K-12. This formula contains four regions defined from analyses of LPS from four key strains, the parent and mutants which had lost one, two, or three regions of their carbohydrates. Support for the formula was derived from the susceptibility of the key mutants to several bacteriophages. One of these, phage phi W, was found specific for strains which had lost region 4. In this paper we described inactivation in vitro of phage phi W and its host-range mutant phi 5, using LPS devoid of regions 2 to 4. The blocking of phi W was found to require about 0.15 M concentrations of monovalent cations and to be inhibited by low concentrations of calcium and magnesium ions. One particle of phage phi W required 2 times 10-16 g of LPS devoid of region 4 for stoichiometric inactivation. Phage phi 5 was blocked by both heptose-less LPS (devoid of regions 2 to 4) and glucose-less LPS (devoid of regions 3 to 4) but was unaffected by LPS devoid of region 4. LPS from a heptose-less mutant of Salmonella minnesota showed the same inactivation ability as did LPS from heptose-less strains of E. coli K-12. Lipid A was prepared from LPS containing all four regions. Such lipid A was found to inactivate phi 5, whereas both the polysaccharide moiety as well as the intact LPS were without effect. It is suggested that lipid A is part of the receptor site for phage phi 5."}
{"id": "PMID:1089630", "title": "Genetic analysis of thymidine-resistant and low-thymine-requiring mutants of Escherichia coli K-12 induced by bacteriophage Mu-1.", "content": "Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymidylate synthetase and also thymidine-resistant mutants of a dra-strain, it has been possible to select for strains in which Mu-1 is inserted in this gene cluster. Making use of the polar effect of Mu-induced mutations on more distal genes in the same transcriptional unit, evidence is presented that dra and tpp are co-transcribed from a promoter to the left of dra, and drm and pup are co-transcribed from a promotor located between tpp and drm. Residual levels of purine nucleoside phosphorylase in drm- mutants induced by phage Mu seem to indicate that a weak promotor lies between drm and pup. From a strain in which Mu-1 is inserted in drm, a mutant has been isolated that has a deletion extending into tpp. Since this strain lacks thymidylate synthetase, it is unable to grow on minimal medium containing thymine. Mutants isolated from this strain that can grow on minimal medium containing thymine have been shown to have increased levels of the enzyme uridine phosphorylase.", "contents": "Genetic analysis of thymidine-resistant and low-thymine-requiring mutants of Escherichia coli K-12 induced by bacteriophage Mu-1. Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymidylate synthetase and also thymidine-resistant mutants of a dra-strain, it has been possible to select for strains in which Mu-1 is inserted in this gene cluster. Making use of the polar effect of Mu-induced mutations on more distal genes in the same transcriptional unit, evidence is presented that dra and tpp are co-transcribed from a promoter to the left of dra, and drm and pup are co-transcribed from a promotor located between tpp and drm. Residual levels of purine nucleoside phosphorylase in drm- mutants induced by phage Mu seem to indicate that a weak promotor lies between drm and pup. From a strain in which Mu-1 is inserted in drm, a mutant has been isolated that has a deletion extending into tpp. Since this strain lacks thymidylate synthetase, it is unable to grow on minimal medium containing thymine. Mutants isolated from this strain that can grow on minimal medium containing thymine have been shown to have increased levels of the enzyme uridine phosphorylase."}
{"id": "PMID:1089631", "title": "Effect of a leu-linked mutation on the valine sensitivity of acetohydroxy acid synthase activity in Escherichia coli.", "content": "A spontaneous leu-linked mutation (ilvH2015) in Escherichia coli K-12 made the strain resistant to 1 mM valine and l mM glycylvaline (Val-r) and caused the isoleucine and valine biosynthetic enzyme, acetohydroxy acid synthase, to be less sensitive to feedback inhibition by valine than the wild-type enzyme. Transfer of the ilvDAC deletion into a strain carrying ilvH2015 abolished the effect of the marker on the acetohydroxy acid synthase and rendered it as sensitive to valine as the enzyme in the isogenic control strain without the Val-r marker under both repressing and limiting conditions. In contrast, auxotrophy caused by transfer of an ilvC lesion into the Val-r strain did not interfere with the effect of ilvH2015 on valine sensitivity of acetohydroxy acid synthase. In addition, the presence of the Val-r marker produced minor but significant pleiotropic effects on several other isoleucine and valine biosynthetic enzymes but did not cause derepression of the ilv gene cluster. These studies suggest some type of interaction between a product produced by a gene close to leu and the isoleucine and valine biosynthetic enzymes.", "contents": "Effect of a leu-linked mutation on the valine sensitivity of acetohydroxy acid synthase activity in Escherichia coli. A spontaneous leu-linked mutation (ilvH2015) in Escherichia coli K-12 made the strain resistant to 1 mM valine and l mM glycylvaline (Val-r) and caused the isoleucine and valine biosynthetic enzyme, acetohydroxy acid synthase, to be less sensitive to feedback inhibition by valine than the wild-type enzyme. Transfer of the ilvDAC deletion into a strain carrying ilvH2015 abolished the effect of the marker on the acetohydroxy acid synthase and rendered it as sensitive to valine as the enzyme in the isogenic control strain without the Val-r marker under both repressing and limiting conditions. In contrast, auxotrophy caused by transfer of an ilvC lesion into the Val-r strain did not interfere with the effect of ilvH2015 on valine sensitivity of acetohydroxy acid synthase. In addition, the presence of the Val-r marker produced minor but significant pleiotropic effects on several other isoleucine and valine biosynthetic enzymes but did not cause derepression of the ilv gene cluster. These studies suggest some type of interaction between a product produced by a gene close to leu and the isoleucine and valine biosynthetic enzymes."}
{"id": "PMID:1089632", "title": "Mutation affecting regulation of synthesis of acetohydroxy acid synthetase in Escherichia coli K-12.", "content": "Altered regulation of synthesis of acetohydroxy acid synthetase (AHAS) was previously reported in a mutant of Escherichia coli strain K-12. The mutant strain, growing in minimal medium, exhibits a partial growth limiatation and derepression of AHAS, owing to deficient synthesis of isoleucine. The genetic lesion (ilvE503) causing the isoleucine limitation was shown to cause derepression of a valine-sensitive AHAS activity. The derepression effect of the ilvE503 mutation upon synthesis of AHAS was conclusively demonstrated by introducing both the ilvE503 allele and an altered AHAS (ilv-521) into the same cell. Evidence is presented that suggests the presence of multiple genetic regions for synthesis and control of the valine-sensitive AHAS activity.", "contents": "Mutation affecting regulation of synthesis of acetohydroxy acid synthetase in Escherichia coli K-12. Altered regulation of synthesis of acetohydroxy acid synthetase (AHAS) was previously reported in a mutant of Escherichia coli strain K-12. The mutant strain, growing in minimal medium, exhibits a partial growth limiatation and derepression of AHAS, owing to deficient synthesis of isoleucine. The genetic lesion (ilvE503) causing the isoleucine limitation was shown to cause derepression of a valine-sensitive AHAS activity. The derepression effect of the ilvE503 mutation upon synthesis of AHAS was conclusively demonstrated by introducing both the ilvE503 allele and an altered AHAS (ilv-521) into the same cell. Evidence is presented that suggests the presence of multiple genetic regions for synthesis and control of the valine-sensitive AHAS activity."}
{"id": "PMID:1089633", "title": "Enzymatic production of deoxyribonucleic acid double-strand breaks after ultraviolet irradiation of Escherichia coli K-12.", "content": "We have observed the enzymatic production of deoxyribonucleic acid (DNA) doublestrand breaks in Escherichia coli K12 after ultraviolet irradiation. Doublestrand breaks appeared in wild-type, polA1, recB21, recA, and exrA strains after incubation in minimal medium. THE UVRA6 strain showed no evidence of double-strand breakage under the same conditions. Our data suggest that uvr+ cells, which are proficient in the incision step of excision repair, accumulate double-strand breaks in their DNA as a result of the excision repair process, i.e., arising from closely matched incisions, excision gaps, or incisions and gaps on opposite strands of the DNA twin helix. Furthermore, strains deficient in excision repair subsequent to the incision step (i.e., polA, rec, exrA) showed more double-strand breaks than the wild type strain. The results raise the possibility that a significant fraction of the lethal events in ultraviolet-irradiated, repair-proficient (uvr+) cell may be enzymatically-induced DNA double-strand breaks.", "contents": "Enzymatic production of deoxyribonucleic acid double-strand breaks after ultraviolet irradiation of Escherichia coli K-12. We have observed the enzymatic production of deoxyribonucleic acid (DNA) doublestrand breaks in Escherichia coli K12 after ultraviolet irradiation. Doublestrand breaks appeared in wild-type, polA1, recB21, recA, and exrA strains after incubation in minimal medium. THE UVRA6 strain showed no evidence of double-strand breakage under the same conditions. Our data suggest that uvr+ cells, which are proficient in the incision step of excision repair, accumulate double-strand breaks in their DNA as a result of the excision repair process, i.e., arising from closely matched incisions, excision gaps, or incisions and gaps on opposite strands of the DNA twin helix. Furthermore, strains deficient in excision repair subsequent to the incision step (i.e., polA, rec, exrA) showed more double-strand breaks than the wild type strain. The results raise the possibility that a significant fraction of the lethal events in ultraviolet-irradiated, repair-proficient (uvr+) cell may be enzymatically-induced DNA double-strand breaks."}
{"id": "PMID:1089634", "title": "mut-25, a mutation to mutator linked to purA in Escherichia coli.", "content": "The mutation mut-25 that results in a mutator phenotype is closely linked to purA on the chromosome of Escherichia coli. The gene order in this region is ampA mut-25 purA. purA mut-25 double mutants retained mutator activity indicating that mut-25 is not a mutation in the purA gene. The repair mutations uvrA6, recA56, and exrA1 had no effect on mutation frequencies in mut-25 strains, and mut-25 strains were normally resistant to ultraviolet irradiation. Frequencies of host range mutations were not increased in phages T1, T2, and T7 grown on mut-25 strains. mut-25 could act trans, reverting the trpA46 mutation either on the chromosome or on an F episome. The transitions AT yields GC (adenine-thymine yields guanine-cytosine) and GC yields AT were induced by mut-25.", "contents": "mut-25, a mutation to mutator linked to purA in Escherichia coli. The mutation mut-25 that results in a mutator phenotype is closely linked to purA on the chromosome of Escherichia coli. The gene order in this region is ampA mut-25 purA. purA mut-25 double mutants retained mutator activity indicating that mut-25 is not a mutation in the purA gene. The repair mutations uvrA6, recA56, and exrA1 had no effect on mutation frequencies in mut-25 strains, and mut-25 strains were normally resistant to ultraviolet irradiation. Frequencies of host range mutations were not increased in phages T1, T2, and T7 grown on mut-25 strains. mut-25 could act trans, reverting the trpA46 mutation either on the chromosome or on an F episome. The transitions AT yields GC (adenine-thymine yields guanine-cytosine) and GC yields AT were induced by mut-25."}
{"id": "PMID:1089635", "title": "Suppression of initiation-negative strains of Escherichia coli by integration of the sex factor F.", "content": "Data are presented suggesting that the most critical factor determining whether an Hfr dnaAts strain can synthesize deoxyribonucleic acid and form colonies at temperatures that are nonpermissive for corresponding F- strains is neither the site of insertion of F nor the presence of additional mutations in the F particle or the chromosome; it is whether the particle is capable of autonomous replication at the temperature used. Consequently, suppression of the DnaA phenotype in Hfr strains occurs at 40 C but not, in most of them, at 42 C without the occurrence of additional mutations. The site of insertion of F may also be important since it is shown that in one Hfr dnaA strain partial suppression does occur at 42 C. In addition, it is shown that strains exhibiting suppression by integration of F at 40 C on minimal agar plates do not do so at this temperature on nutrient agar plates.", "contents": "Suppression of initiation-negative strains of Escherichia coli by integration of the sex factor F. Data are presented suggesting that the most critical factor determining whether an Hfr dnaAts strain can synthesize deoxyribonucleic acid and form colonies at temperatures that are nonpermissive for corresponding F- strains is neither the site of insertion of F nor the presence of additional mutations in the F particle or the chromosome; it is whether the particle is capable of autonomous replication at the temperature used. Consequently, suppression of the DnaA phenotype in Hfr strains occurs at 40 C but not, in most of them, at 42 C without the occurrence of additional mutations. The site of insertion of F may also be important since it is shown that in one Hfr dnaA strain partial suppression does occur at 42 C. In addition, it is shown that strains exhibiting suppression by integration of F at 40 C on minimal agar plates do not do so at this temperature on nutrient agar plates."}
{"id": "PMID:1089636", "title": "Positive selection of general amino acid permease mutants in Saccharomyces cerevisiae.", "content": "It was found that D-stereoisomers of natural amino acids inhibit the growth of Saccharomyces cerevisiae cells. Kinetic and genetic evidence showed that d-amino acids enter the cell by the general amino acid permease. Two classes of S. cerevisiae mutants resistant to d-amino acids were isolated. One class of mutants appeared to be defective in the general amino acid permease specified by the gene gap. In the second class, the activity of general amino acid permease was affected by ammonium ions. Mutants of the second class were isolated in a yeast strain with the general amino acid permease insensitive to the presence of ammonium ions in culture media. The mutation affecting the permease, amc, occurred in a locus unlinked to gap.", "contents": "Positive selection of general amino acid permease mutants in Saccharomyces cerevisiae. It was found that D-stereoisomers of natural amino acids inhibit the growth of Saccharomyces cerevisiae cells. Kinetic and genetic evidence showed that d-amino acids enter the cell by the general amino acid permease. Two classes of S. cerevisiae mutants resistant to d-amino acids were isolated. One class of mutants appeared to be defective in the general amino acid permease specified by the gene gap. In the second class, the activity of general amino acid permease was affected by ammonium ions. Mutants of the second class were isolated in a yeast strain with the general amino acid permease insensitive to the presence of ammonium ions in culture media. The mutation affecting the permease, amc, occurred in a locus unlinked to gap."}
{"id": "PMID:1089637", "title": "Urea transport in Saccharomyces cerevisiae.", "content": "Urea transport in Saccharomyces cerevisiae occurs by two pathways. The first mode of uptake is via an active transport system which: (i) has an apparent Km value of 14 muM, (ii) is absolutely dependent upon energy metabolism, (iii) requires pre-growth of the cultures in the presence of oxaluric acid, gratuitous inducer of the allantoin degradative enzymes, and (iv) is sensitive to nitrogen repression. The second mode of uptake which occurs at external urea concentrations in excess of 0.5 mM is via either passive or facilitated diffusion.", "contents": "Urea transport in Saccharomyces cerevisiae. Urea transport in Saccharomyces cerevisiae occurs by two pathways. The first mode of uptake is via an active transport system which: (i) has an apparent Km value of 14 muM, (ii) is absolutely dependent upon energy metabolism, (iii) requires pre-growth of the cultures in the presence of oxaluric acid, gratuitous inducer of the allantoin degradative enzymes, and (iv) is sensitive to nitrogen repression. The second mode of uptake which occurs at external urea concentrations in excess of 0.5 mM is via either passive or facilitated diffusion."}
{"id": "PMID:1089638", "title": "Genetic and phenotypic characterization of dnaC mutations.", "content": "The dna-1, dna-2, dna-7, and dna-28 mutations, all of which are located near min 89.5 on the E. coli linkage map, have been characterized further. As previously demonstrated for dna-2 and dna-28, neither the dna-1 nor dna-7 mutation affects the ability of a strain to produce bacteriophage lambda at temperatures non-permissive for the continued replication of the bacterial chromosome. The reported temperature-sensitive inhibition of lambda production in a strain carrying dna-7 is shown to be a consequence of a thermosensitive host specificity mutation in the hsm gene and not of the dna-7 mutation. The four dna mutations are recessive to the wild type and define a single dnaC cistron according to standard complementation criteria. Unlike other characterized dnaC mutants, however, strains carrying the dnaC1 or dnaC7 alleles exhibit an abrupt cessation of deoxyribonucleic acid synthesis at 42 C that appears to be more compatible with a defect in deoxyribonucleic acid chain elongation rather than in initiation. The possibility that the apparent elongation defect is actually a composite effect of residual synthesis and deoxyribonucleic acid degradation is raised by the net deoxyribonucleic acid degradation observed in the dnaC1 strain at 42 C. Several alternative possibilities for the function of the dnaC gene product are suggested.", "contents": "Genetic and phenotypic characterization of dnaC mutations. The dna-1, dna-2, dna-7, and dna-28 mutations, all of which are located near min 89.5 on the E. coli linkage map, have been characterized further. As previously demonstrated for dna-2 and dna-28, neither the dna-1 nor dna-7 mutation affects the ability of a strain to produce bacteriophage lambda at temperatures non-permissive for the continued replication of the bacterial chromosome. The reported temperature-sensitive inhibition of lambda production in a strain carrying dna-7 is shown to be a consequence of a thermosensitive host specificity mutation in the hsm gene and not of the dna-7 mutation. The four dna mutations are recessive to the wild type and define a single dnaC cistron according to standard complementation criteria. Unlike other characterized dnaC mutants, however, strains carrying the dnaC1 or dnaC7 alleles exhibit an abrupt cessation of deoxyribonucleic acid synthesis at 42 C that appears to be more compatible with a defect in deoxyribonucleic acid chain elongation rather than in initiation. The possibility that the apparent elongation defect is actually a composite effect of residual synthesis and deoxyribonucleic acid degradation is raised by the net deoxyribonucleic acid degradation observed in the dnaC1 strain at 42 C. Several alternative possibilities for the function of the dnaC gene product are suggested."}
{"id": "PMID:1089639", "title": "Growth of bacteriophages MS2 and T7 on streptomycin-resistant mutants of Escherichia coli.", "content": "Streptomycin-resistant mutants of an Hfr strain of Escherichia coli K were examined for their ability to support the growth of male-specific ribonucleic acid phage MS2 and female-specific deoxyribonucleic acid phage T7. Normally, the Hfr strain allows propagation of MS2 and is lysed by it (efficiency of plating equal to 1), whereas the same strain restricts propagation of T7 and is not lysed by it (efficiency of plating smaller than 10-7). Twenty-four isolates out of 26 independently obtained streptomycin-resistant mutants are partially or completely derestricted for propagation of T7; efficiency of plating of T7 in such strains ranges from 10-3-1. Depending on their response to plating of MS2 and T7, the streptomycin-resistant mutants can be divided into four classes. The mutants in all four classes continue to be \"male\" in conjugation with F- strains. Genetic analysis is presented to show that restriction of MS2, derestriction of T7, and resistance to streptomycin are the pleiotropic effects of a single mutation at the strA locus.", "contents": "Growth of bacteriophages MS2 and T7 on streptomycin-resistant mutants of Escherichia coli. Streptomycin-resistant mutants of an Hfr strain of Escherichia coli K were examined for their ability to support the growth of male-specific ribonucleic acid phage MS2 and female-specific deoxyribonucleic acid phage T7. Normally, the Hfr strain allows propagation of MS2 and is lysed by it (efficiency of plating equal to 1), whereas the same strain restricts propagation of T7 and is not lysed by it (efficiency of plating smaller than 10-7). Twenty-four isolates out of 26 independently obtained streptomycin-resistant mutants are partially or completely derestricted for propagation of T7; efficiency of plating of T7 in such strains ranges from 10-3-1. Depending on their response to plating of MS2 and T7, the streptomycin-resistant mutants can be divided into four classes. The mutants in all four classes continue to be \"male\" in conjugation with F- strains. Genetic analysis is presented to show that restriction of MS2, derestriction of T7, and resistance to streptomycin are the pleiotropic effects of a single mutation at the strA locus."}
{"id": "PMID:1089640", "title": "Ultraviolet light sensitivity of Escherichia coli K-12 strains carrying ruv mutations in combination with uvrA or lon mutant alleles.", "content": "Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ultraviolet light as the ruv minus single mutant.", "contents": "Ultraviolet light sensitivity of Escherichia coli K-12 strains carrying ruv mutations in combination with uvrA or lon mutant alleles. Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ultraviolet light as the ruv minus single mutant."}
{"id": "PMID:1089641", "title": "Nucleotide pools and regulation of ribonucleic acid synthesis in yeast.", "content": "Nucleotide pools were measured in growing and amino acid-starved Saccharomyces cerevisiae. During amino acid starvation there are neither significant changes in the endogeneous nucleoside triphosphate pool levels nor measurable synthesis of guanosine 5'-diphosphate, 3'-diphosphate. Stable ribonucleic acid synthesis does not appear to be regulated by changes in the triphosphate pools or by the unusual nucleotide guanosine 5'-diphosphate, 3'-diphosphate.", "contents": "Nucleotide pools and regulation of ribonucleic acid synthesis in yeast. Nucleotide pools were measured in growing and amino acid-starved Saccharomyces cerevisiae. During amino acid starvation there are neither significant changes in the endogeneous nucleoside triphosphate pool levels nor measurable synthesis of guanosine 5'-diphosphate, 3'-diphosphate. Stable ribonucleic acid synthesis does not appear to be regulated by changes in the triphosphate pools or by the unusual nucleotide guanosine 5'-diphosphate, 3'-diphosphate."}
{"id": "PMID:1089642", "title": "Sensitive methods for the detection and characterization of double helical ribonucleic acid.", "content": "We have evaluated three methods which respond specifically to stable RNA-RNA duplexes and have compared their utility for examining several sorts of nucleic acids. We find that these methods, stepwise chromatography on Whatman CF11-cellulose; digestion with Escherichia coli RNase III; and specific inhibition of globin synthesis in vitro in rabbit reticulocyte lysates, are able to distinguish between stable double-stranded RNA and single-stranded RNA in the expected manner. The most sensitive method, inhibition of globin synthesis, responds to double-stranded RNA concentrations below 0.1 ng per ml. We have used the predominantly single-stranded RNA from several RNA bacteriophages of E. coli to test both the sensitivity and selectivity of these methods. The three viral RNAs tested contain low levels of double-stranded RNA which can be readily removed, leaving RNA which is not recognized as double-stranded RNA, despite indications from physical and sequencing studies that secondary structure is present. In particular, a potential hairpir loop of known sequence has been isolated from phage f2 RNA. Its properties were found to depart significantly from those of RNA-RNA duplexes by those two of our three methods capable of testing RNA of this size. Analysis of two eukaryotic mRNA populations by these methods was complicated by the presence of poly(A). Synthetic poly(A) chromatographs like double-stranded RNA on cellulose CF11 columns, and we could distinguish it from reovirus double-stranded RNA only at elevated temperatures.", "contents": "Sensitive methods for the detection and characterization of double helical ribonucleic acid. We have evaluated three methods which respond specifically to stable RNA-RNA duplexes and have compared their utility for examining several sorts of nucleic acids. We find that these methods, stepwise chromatography on Whatman CF11-cellulose; digestion with Escherichia coli RNase III; and specific inhibition of globin synthesis in vitro in rabbit reticulocyte lysates, are able to distinguish between stable double-stranded RNA and single-stranded RNA in the expected manner. The most sensitive method, inhibition of globin synthesis, responds to double-stranded RNA concentrations below 0.1 ng per ml. We have used the predominantly single-stranded RNA from several RNA bacteriophages of E. coli to test both the sensitivity and selectivity of these methods. The three viral RNAs tested contain low levels of double-stranded RNA which can be readily removed, leaving RNA which is not recognized as double-stranded RNA, despite indications from physical and sequencing studies that secondary structure is present. In particular, a potential hairpir loop of known sequence has been isolated from phage f2 RNA. Its properties were found to depart significantly from those of RNA-RNA duplexes by those two of our three methods capable of testing RNA of this size. Analysis of two eukaryotic mRNA populations by these methods was complicated by the presence of poly(A). Synthetic poly(A) chromatographs like double-stranded RNA on cellulose CF11 columns, and we could distinguish it from reovirus double-stranded RNA only at elevated temperatures."}
{"id": "PMID:1089643", "title": "Deoxyribonucleic acid polymerase III of Escherichia coli. Purification and properties.", "content": "DNA polymerase III has been purified 4,500-fold from the Escherichis coli mutant, HMS83, which lacks DNA polymerases I and II. When subjected to disc gel electrophoresis, the most purified fraction exhibits a single major protein band from which enzymatic activity may be recovered. Polyacrylamide gel electrophoresis under denaturing conditions produces two protein bands with molecular weights of 140,000 and 40,000. The sedimentation coefficient of the enzyme is 7.0 S, and the Stokes radius is 62 A. Taken together these tow parameters indicate a native molecular weight of 180,000. Purified DNA polymerase III catalyzes the polymerization of nucleotides into DNA when provided with both a DNA template and a complementary primer strand. The newly synthesized DNA is covalently attached to the 3' terminus of the primer strand. Because the extent of polymerization is only 10 to 100 nucleotides, the best substrates are native DNA molecules with small single-stranded regions. The most purified enzyme preparation is devoid of endonuclease activities. In addition to the two exonuclease activities described in the accompanying paper, purified polymerase III also catalyzes pyrophosphorolysis and the exchange of pyrophosphate into deoxynucleoside triphosphates. DNA polymerase III has also been isolated from wild type E. coli containing the other two known DNA polymerases. Futhermore, the enzyme purified from three different polC mutants exhibits altered polymerase III activity, confirming that polC is the structural gene for DNA polymerase III (Gefter, M., Hirota, Y., Kornberb, T., Wechsler, J., and Barnoux, C. (1971) Proc. Natl. Acad. Sci. U. S. A. 68, 3150-3153).", "contents": "Deoxyribonucleic acid polymerase III of Escherichia coli. Purification and properties. DNA polymerase III has been purified 4,500-fold from the Escherichis coli mutant, HMS83, which lacks DNA polymerases I and II. When subjected to disc gel electrophoresis, the most purified fraction exhibits a single major protein band from which enzymatic activity may be recovered. Polyacrylamide gel electrophoresis under denaturing conditions produces two protein bands with molecular weights of 140,000 and 40,000. The sedimentation coefficient of the enzyme is 7.0 S, and the Stokes radius is 62 A. Taken together these tow parameters indicate a native molecular weight of 180,000. Purified DNA polymerase III catalyzes the polymerization of nucleotides into DNA when provided with both a DNA template and a complementary primer strand. The newly synthesized DNA is covalently attached to the 3' terminus of the primer strand. Because the extent of polymerization is only 10 to 100 nucleotides, the best substrates are native DNA molecules with small single-stranded regions. The most purified enzyme preparation is devoid of endonuclease activities. In addition to the two exonuclease activities described in the accompanying paper, purified polymerase III also catalyzes pyrophosphorolysis and the exchange of pyrophosphate into deoxynucleoside triphosphates. DNA polymerase III has also been isolated from wild type E. coli containing the other two known DNA polymerases. Futhermore, the enzyme purified from three different polC mutants exhibits altered polymerase III activity, confirming that polC is the structural gene for DNA polymerase III (Gefter, M., Hirota, Y., Kornberb, T., Wechsler, J., and Barnoux, C. (1971) Proc. Natl. Acad. Sci. U. S. A. 68, 3150-3153)."}
{"id": "PMID:1089644", "title": "Fluorescence studies on the active sites of porcine pepsin and Rhizopus-pepsin.", "content": "Fluorescence studies on the interaction, with porcine pepsin, of oligopeptides bearing a mansyl (Mns, 6-(N-methylanilino)-2-naphthalenesulfonyl) or dansyl (Dns, 5-dimethylaminonaphthalene-1-sulfonyl) group at the NH2 or COOH terminus have provided further evidence showing that the probe group is drawn into the extended active site largely as a consequence of the specific binding of the peptide portion of the substrate. The active site does not appear to have appreciable intrinsic affinity for the mansyl or dansyl group, and the principal contribution to the specific peptide-protein interaction is provided by the sensitive L-phenylalanyl-L-phenylalanyl (Phe-Phe) unit of the substrates tested. The pepsin inhibitor pepstatin can displace substrates such as Mns-(Gly)n-Phe-Phe-OR or Gly-Gly-Phe-Phe-NHNH-Mns from the active site of porcine pepsin; in these circumstances the mansyl group is bound weakly at a separate, nonspecific locus, distinct from the active site, which can accept the mansyl group of Mns-Gly-Gly-OR or mansylamide. In the interaction with substrates such as Mns-(Gly)n-Phe-Phe-OR or Dns-(Gly)n-Phe-Phe-OR, the above conclusions for porcine pepsin also apply to Rhizopus-pepsin. With substrates such as Gly-Gly-Phe-Phe-NHNH-Mns, however, the active site of Rhizopus-pepsin shows less affinity for the fluorescent probe group than does that of porcine pepsin, suggesting structural differences between the two acid proteinases in the region of their extended active sites which bind the COOH-terminal portion of small oligopeptide substrates.", "contents": "Fluorescence studies on the active sites of porcine pepsin and Rhizopus-pepsin. Fluorescence studies on the interaction, with porcine pepsin, of oligopeptides bearing a mansyl (Mns, 6-(N-methylanilino)-2-naphthalenesulfonyl) or dansyl (Dns, 5-dimethylaminonaphthalene-1-sulfonyl) group at the NH2 or COOH terminus have provided further evidence showing that the probe group is drawn into the extended active site largely as a consequence of the specific binding of the peptide portion of the substrate. The active site does not appear to have appreciable intrinsic affinity for the mansyl or dansyl group, and the principal contribution to the specific peptide-protein interaction is provided by the sensitive L-phenylalanyl-L-phenylalanyl (Phe-Phe) unit of the substrates tested. The pepsin inhibitor pepstatin can displace substrates such as Mns-(Gly)n-Phe-Phe-OR or Gly-Gly-Phe-Phe-NHNH-Mns from the active site of porcine pepsin; in these circumstances the mansyl group is bound weakly at a separate, nonspecific locus, distinct from the active site, which can accept the mansyl group of Mns-Gly-Gly-OR or mansylamide. In the interaction with substrates such as Mns-(Gly)n-Phe-Phe-OR or Dns-(Gly)n-Phe-Phe-OR, the above conclusions for porcine pepsin also apply to Rhizopus-pepsin. With substrates such as Gly-Gly-Phe-Phe-NHNH-Mns, however, the active site of Rhizopus-pepsin shows less affinity for the fluorescent probe group than does that of porcine pepsin, suggesting structural differences between the two acid proteinases in the region of their extended active sites which bind the COOH-terminal portion of small oligopeptide substrates."}
{"id": "PMID:1089645", "title": "The structural basis for the resistance of Escherichia coli formylmethionyl transfer ribonucleic acid to cleavage by Escherichia coli peptidyl transfer ribonucleic acid hydrolase.", "content": "Escherichia coli formylmethionly-tRNA-tMet is unique among N-acylaminoacyl-tRNAs in its resistance to cleavage by peptidyl-tRNA hydrolase. Chemical modification of tRNA-fMet with sodium bisulfite converts fMet-tRNA-fMet into a good substrate for the hydrolase. The products of the enzymatic cleavage are free tRNA-fMet and formylmethionine. Bisulfite treatment produces cytidine to uridine base changes at several sites in the tRNA structure. One of these modifications results in formation of a new hydrogen-bonded base pair at the end of the acceptor stem of tRNA-fMet. We have shown that this modification is responsible for the observed change in biological activity. Enzymatic cleavage appears to be facilitated by the presence of a 5-terminal phosphate at the end of a fully base-paired acceptor stem, because removal of the 5-phosphate group from N-acetylphenylalanyl-tRNA-Phe or bisulfite-modified fMet-tRNA-FMet reduced the rate of hydrolysis of these substrates. The unpaired base at the 5 terminus of unmodified fMet-tRNA-fMet appears to reduce susceptibility of the tRNA to hydrolytic attack both by positioning the 5-phosphate in an unfavorable orientation and by directly interfering with enzymatic binding. The unusual structure of the acceptor stem of this E. coli tRNA thus plays a critical role in maintaining the viability of the organism by preventing enzymatic cleavage of the fMet group from the bacterial initiator tRNA.", "contents": "The structural basis for the resistance of Escherichia coli formylmethionyl transfer ribonucleic acid to cleavage by Escherichia coli peptidyl transfer ribonucleic acid hydrolase. Escherichia coli formylmethionly-tRNA-tMet is unique among N-acylaminoacyl-tRNAs in its resistance to cleavage by peptidyl-tRNA hydrolase. Chemical modification of tRNA-fMet with sodium bisulfite converts fMet-tRNA-fMet into a good substrate for the hydrolase. The products of the enzymatic cleavage are free tRNA-fMet and formylmethionine. Bisulfite treatment produces cytidine to uridine base changes at several sites in the tRNA structure. One of these modifications results in formation of a new hydrogen-bonded base pair at the end of the acceptor stem of tRNA-fMet. We have shown that this modification is responsible for the observed change in biological activity. Enzymatic cleavage appears to be facilitated by the presence of a 5-terminal phosphate at the end of a fully base-paired acceptor stem, because removal of the 5-phosphate group from N-acetylphenylalanyl-tRNA-Phe or bisulfite-modified fMet-tRNA-FMet reduced the rate of hydrolysis of these substrates. The unpaired base at the 5 terminus of unmodified fMet-tRNA-fMet appears to reduce susceptibility of the tRNA to hydrolytic attack both by positioning the 5-phosphate in an unfavorable orientation and by directly interfering with enzymatic binding. The unusual structure of the acceptor stem of this E. coli tRNA thus plays a critical role in maintaining the viability of the organism by preventing enzymatic cleavage of the fMet group from the bacterial initiator tRNA."}
{"id": "PMID:1089646", "title": "Subunit interactions in aspartate transcarbamylase. The interaction between catalytic and regulatory subunits and the effect of ligands.", "content": "The interaction between the catalytic subunit (c3) and the regulatory subunit (r2) of aspartate transcarbamylase from Escherichia coli was studied by measuring the reversible formation of the c3r6 complex as a function of r2 concentration. Conversion to the native enzyme was prevented by using a very low concentration of c2 (40 ng per ml) in the presence of bovine serum albumin. A simple hyperbolic r2 saturation curve was obtained suggesting the presence of only one kind of c:r domain. From the association constant for the formation of c3r6, the free energy of c:r interaction can be estimated to be about -10 Cal per mole. Neither CTP nor ATP appears to affect the strength of c:r interaction in this complex. Succinate in the presence of carbamyl phosphate promotes tighter binding. At higher concentration of c3 and nonsaturating levels of r2, conversion to the native enzyme (c3r6) takes place. This renaturation process is second order with respect to the concentration of c3 and is virtually irreversible. Renaturation is inhibited by saturating levels of r2 and to some extent by both CTP and ATP. The effect of ligands on c:r interactions reported here may have significance in the allosteric mechanism of the native enzyme.", "contents": "Subunit interactions in aspartate transcarbamylase. The interaction between catalytic and regulatory subunits and the effect of ligands. The interaction between the catalytic subunit (c3) and the regulatory subunit (r2) of aspartate transcarbamylase from Escherichia coli was studied by measuring the reversible formation of the c3r6 complex as a function of r2 concentration. Conversion to the native enzyme was prevented by using a very low concentration of c2 (40 ng per ml) in the presence of bovine serum albumin. A simple hyperbolic r2 saturation curve was obtained suggesting the presence of only one kind of c:r domain. From the association constant for the formation of c3r6, the free energy of c:r interaction can be estimated to be about -10 Cal per mole. Neither CTP nor ATP appears to affect the strength of c:r interaction in this complex. Succinate in the presence of carbamyl phosphate promotes tighter binding. At higher concentration of c3 and nonsaturating levels of r2, conversion to the native enzyme (c3r6) takes place. This renaturation process is second order with respect to the concentration of c3 and is virtually irreversible. Renaturation is inhibited by saturating levels of r2 and to some extent by both CTP and ATP. The effect of ligands on c:r interactions reported here may have significance in the allosteric mechanism of the native enzyme."}
{"id": "PMID:1089647", "title": "Subunit interactions in aspartate transcarbamylase. A model for the allosteric mechanism.", "content": "The conformational changes in aspartate transcarbamylase upon binding of substrates or regulatory ligands and the effects of alterations in the subunit structure on the allosteric interactions are reviewed. The available information including recent results from studies of the c3r6 complex (c denotes the catalytic polypeptide and r, the regulatory polypeptide) is considered in terms of the existing models for the discrepancies between experimental observations and the present models could be resolved by postulating an important role for r:r interactions in the allosteric mechanism. A new model is presented in which an obligatory conformational change upon binding of substrates results in an alteration in the relative orientation of c versus r. As a consequence of symmetry conservation, the r:r domain is shifted to a position of higher potential energy. By favoring one or the other alternative r:r domains, CTP and ATP can respectively enhance and reduce the sigmoidal character of substrate saturation. The model is shown to be consistent with all of the important known properties of the enzyme. Because the heterotropic effects of CTP or ATP are postulated to operate via a mechanism separate from that for the homotropic effects of the substrates, this model accounts satisfactorily for the observation by Kerbiriou and Herve (Kerbiriou, D., and Herve, G. (1973) J. Mol. Biol. 78, 687-702) that homotropic effects can be abolished whereas heterotropic effects are retained in the altered enzyme from Escherichia coli grown in the presence of 2-thiouracil.", "contents": "Subunit interactions in aspartate transcarbamylase. A model for the allosteric mechanism. The conformational changes in aspartate transcarbamylase upon binding of substrates or regulatory ligands and the effects of alterations in the subunit structure on the allosteric interactions are reviewed. The available information including recent results from studies of the c3r6 complex (c denotes the catalytic polypeptide and r, the regulatory polypeptide) is considered in terms of the existing models for the discrepancies between experimental observations and the present models could be resolved by postulating an important role for r:r interactions in the allosteric mechanism. A new model is presented in which an obligatory conformational change upon binding of substrates results in an alteration in the relative orientation of c versus r. As a consequence of symmetry conservation, the r:r domain is shifted to a position of higher potential energy. By favoring one or the other alternative r:r domains, CTP and ATP can respectively enhance and reduce the sigmoidal character of substrate saturation. The model is shown to be consistent with all of the important known properties of the enzyme. Because the heterotropic effects of CTP or ATP are postulated to operate via a mechanism separate from that for the homotropic effects of the substrates, this model accounts satisfactorily for the observation by Kerbiriou and Herve (Kerbiriou, D., and Herve, G. (1973) J. Mol. Biol. 78, 687-702) that homotropic effects can be abolished whereas heterotropic effects are retained in the altered enzyme from Escherichia coli grown in the presence of 2-thiouracil."}
{"id": "PMID:1089648", "title": "Ethidium binding affinity of circular lambda deoxyribonucleic acid determined fluorometrically.", "content": "Ethidium-binding isotherms for purified circular lambda DNA, isolated from a superinfected lysogen, and for linear lambda DNA, isolated from the purified phage, were constructed from fluorescence measurements of ethidium-DNA MIXTURES. The measurements were made in 0.01 M Tris-HC1-0.001 M EDTA,pH 7.1, buffer at 20 degrees and in the same buffer containing 0.1, 0.4, or 1.0 M NaC1. When NaC1 was present, differences in the binding affinity for supercoiled and linear DNA could be quantitated. As the ethidium concentration was increased, supercoiled lambda DNA molecules bound the intercalating dye first more and then less avidly than nonsupercoiled ones. The number of potential supercoils in a circular lambda DNA molecular in the absence of dye was calculated from the amount of dye bound when it exhibited the same affinity for dye as its linear counterpart. The point of equivalent affinity shifted from 0.053 mol of dye bound per mol of nucleotide in 0.1 M NaC1 to 0.067 mol in 1.0 M NaC1. This corresponds to the removal of 164 and 206 supercoiling turns per molecule and superhelix densities in the absence of dye equal to 0.036 and 0.045 superhelical turns per 10 base pairs. If this difference in the number of supercoils reflects a salt-dependent change in the average rotation angle between base pairs of the Watson-Crick helix the angle differs by 0.32% in the two ionic environments.", "contents": "Ethidium binding affinity of circular lambda deoxyribonucleic acid determined fluorometrically. Ethidium-binding isotherms for purified circular lambda DNA, isolated from a superinfected lysogen, and for linear lambda DNA, isolated from the purified phage, were constructed from fluorescence measurements of ethidium-DNA MIXTURES. The measurements were made in 0.01 M Tris-HC1-0.001 M EDTA,pH 7.1, buffer at 20 degrees and in the same buffer containing 0.1, 0.4, or 1.0 M NaC1. When NaC1 was present, differences in the binding affinity for supercoiled and linear DNA could be quantitated. As the ethidium concentration was increased, supercoiled lambda DNA molecules bound the intercalating dye first more and then less avidly than nonsupercoiled ones. The number of potential supercoils in a circular lambda DNA molecular in the absence of dye was calculated from the amount of dye bound when it exhibited the same affinity for dye as its linear counterpart. The point of equivalent affinity shifted from 0.053 mol of dye bound per mol of nucleotide in 0.1 M NaC1 to 0.067 mol in 1.0 M NaC1. This corresponds to the removal of 164 and 206 supercoiling turns per molecule and superhelix densities in the absence of dye equal to 0.036 and 0.045 superhelical turns per 10 base pairs. If this difference in the number of supercoils reflects a salt-dependent change in the average rotation angle between base pairs of the Watson-Crick helix the angle differs by 0.32% in the two ionic environments."}
{"id": "PMID:1089649", "title": "Purification of closed circular lambda deoxyribonucleic acid and its sedimentation properties as a function of Sodium chloride concentration and ethidium binding.", "content": "The sedimentation of circular lambda DNA suggests that the molecular undergoes significant changes in shape and super-coiling as the NaC1 concentration increases. Closed circular lambda DNA, species I, isolated and purified from superinfected immune bacteria, sediments in sucrose gradients of low ionic strength at a rate 2.0 times faster than linear lambda DNA, species III. The addition of ethidium causes the sedimentation rate of species I DNA to decrease until enough dye is bound to remove 121 supercoils per molecule. At this point, species I co-sediments with nicked and nonsupercoiled species II. Futher additions of ethidium cause the sedimentation rate to increase until the relative rate of species I is again at least twice that of species III. This classical behavior is altered when NaC1 is present in the buffer. In 1.0 M NaC1 the changes in S are complex. Initially, species I sediments 1.55 times faster than species III. Titration with ethidium caused a decrease in S to an early minimum value, than an increase to a first maximum, followed by a decrease to the S of species II. At this point enough dye has intercalated to remove 208 superhelical turns. Further additions of dye introduce supercoils and cause S to increase again. In 0.1 to 0.4 M NaC1 the relative S of species I is 1.69 and 1.59, respectively. If titrated with ethidium, S first increases to a maximum value then decreases to the minimum rate when enough dye is bound to remove 158 and 183 supercoils, respectively. The results indicate an increase in the superhelix density from 0.026 turns per 10 base pairs in buffer alone to 0.045 in the same buffer with 1.0 M NaC1. If this change in superhelix density results from a concomitant change in the average rotation angle between base pairs in the Watson-Crick helix, the addition of 1.0 M NaC1 alters the rotation angle by 0.68 degrees per base pair.", "contents": "Purification of closed circular lambda deoxyribonucleic acid and its sedimentation properties as a function of Sodium chloride concentration and ethidium binding. The sedimentation of circular lambda DNA suggests that the molecular undergoes significant changes in shape and super-coiling as the NaC1 concentration increases. Closed circular lambda DNA, species I, isolated and purified from superinfected immune bacteria, sediments in sucrose gradients of low ionic strength at a rate 2.0 times faster than linear lambda DNA, species III. The addition of ethidium causes the sedimentation rate of species I DNA to decrease until enough dye is bound to remove 121 supercoils per molecule. At this point, species I co-sediments with nicked and nonsupercoiled species II. Futher additions of ethidium cause the sedimentation rate to increase until the relative rate of species I is again at least twice that of species III. This classical behavior is altered when NaC1 is present in the buffer. In 1.0 M NaC1 the changes in S are complex. Initially, species I sediments 1.55 times faster than species III. Titration with ethidium caused a decrease in S to an early minimum value, than an increase to a first maximum, followed by a decrease to the S of species II. At this point enough dye has intercalated to remove 208 superhelical turns. Further additions of dye introduce supercoils and cause S to increase again. In 0.1 to 0.4 M NaC1 the relative S of species I is 1.69 and 1.59, respectively. If titrated with ethidium, S first increases to a maximum value then decreases to the minimum rate when enough dye is bound to remove 158 and 183 supercoils, respectively. The results indicate an increase in the superhelix density from 0.026 turns per 10 base pairs in buffer alone to 0.045 in the same buffer with 1.0 M NaC1. If this change in superhelix density results from a concomitant change in the average rotation angle between base pairs in the Watson-Crick helix, the addition of 1.0 M NaC1 alters the rotation angle by 0.68 degrees per base pair."}
{"id": "PMID:1089650", "title": "The nucleotide sequence in the promoter region of the fene for an Escherichia coli tyrosine transfer ribonucleic acid.", "content": "The sequence of 29 nucleotides in the region preceding the initiation of the transcription of the Escherichia coli tyrosine tRNA gene has been determined. This is: (5')  ---GGGGCGCATCATATCAAATGACGCGCCGC--- (3') (3') ---CCCCGCGTAGTATAGTTTACTGCGGCGGCG--- (5'). The general approach used for the sequence determination involved the DNA polymerase I-catalyzed elongation of suitable deoxyribopolynucleotide primers when hybridized to the 1-strand of phi80psuIII plus DNA at the appropriate site. Sequences of the newly grown oligonucleotide chains were determined by a combination of two-dimensional fingerprinting following partial exonucleolytic degradation, nearst neighbor analyses, and determination of pyrimidine tracts. Primer elongations were carried out in a controlled and stepwise manner and the newly grown oligonucleotide chains were kept short by incorporating the following features into the method: (a) the insertion of a ribonucleotide unit at or near the 3' terminus of the primers; (b) the use of a maximum of three nucleoside 5'-triphosphates in the first stage of the elongation reaction isolation of the elongated primer, and its reuse in a second step together with different sets of deoxy-nucleoside triphosphates; and (c) elongation of the primer using all of the four nucleoside triphosphates with one of the triphosphates being supplied in a limiting concentration.", "contents": "The nucleotide sequence in the promoter region of the fene for an Escherichia coli tyrosine transfer ribonucleic acid. The sequence of 29 nucleotides in the region preceding the initiation of the transcription of the Escherichia coli tyrosine tRNA gene has been determined. This is: (5')  ---GGGGCGCATCATATCAAATGACGCGCCGC--- (3') (3') ---CCCCGCGTAGTATAGTTTACTGCGGCGGCG--- (5'). The general approach used for the sequence determination involved the DNA polymerase I-catalyzed elongation of suitable deoxyribopolynucleotide primers when hybridized to the 1-strand of phi80psuIII plus DNA at the appropriate site. Sequences of the newly grown oligonucleotide chains were determined by a combination of two-dimensional fingerprinting following partial exonucleolytic degradation, nearst neighbor analyses, and determination of pyrimidine tracts. Primer elongations were carried out in a controlled and stepwise manner and the newly grown oligonucleotide chains were kept short by incorporating the following features into the method: (a) the insertion of a ribonucleotide unit at or near the 3' terminus of the primers; (b) the use of a maximum of three nucleoside 5'-triphosphates in the first stage of the elongation reaction isolation of the elongated primer, and its reuse in a second step together with different sets of deoxy-nucleoside triphosphates; and (c) elongation of the primer using all of the four nucleoside triphosphates with one of the triphosphates being supplied in a limiting concentration."}
{"id": "PMID:1089651", "title": "Degradation of abnormal proteins in Escherichia coli. Formation of protein inclusions in cells exposed to amino acid analogs.", "content": "Cells of Escherichia coli selectively degrade proteins that have incorporated amino acid analogs. Within 1 hour after exposure of cells to canavanine, 50% of the analog-containing proteins were degraded to acid-soluble form. At the same time, no net loss of canavanine-containing protein occurred from the 100,000 X g supernatant. Instead, most of the proteins containing the analog, unlike normal ones, accumulated in particulate fractions sedimenting at 10,000 X g or 100,000 X g. They were then lost from these fractions concomitant with the degradation of the abnormal proteins. The loss of such proteins from particulate fractions accounted for all of the protein degraded to acid-soluble form. Similar observations were obtained after incorporation of other analogs or puromycin. The 10,000 X g pellets correspond to amorphous dense intracellular granules visible in electron micrographs of cells exposed to canavanine. Upon removal of the analog, these granules disappeared, simultaneously with the degradation of the analog-containing proteins. These pellets do not resemble a degradative organelle, like the lysosome; they are not osmotically sensitive, do not exclude inulin, are not enclosed by a membrane, and do not show autolytic activity. The proteins in the granules could be solubilized by sodium dodecyl sulfate but not by Triton, NaC1, dithiothreitol, RNase, DNase, or phospholipase. The proteins extracted from the pellet with sodium dodecyl sulfate tend to become particulate again upon removal of this detergent. Incorporation of canavanine caused a normally soluble polypeptide, the monomer of beta-galactosidase, to be inactive and found in the sedimentable fraction. These findings suggest that (a) the presence of amino acid analogs in proteins can make them less soluble, and (b) the inclusions are formed by the spontaneous precipitation of abnormal proteins rather than by an active granule-forming process.", "contents": "Degradation of abnormal proteins in Escherichia coli. Formation of protein inclusions in cells exposed to amino acid analogs. Cells of Escherichia coli selectively degrade proteins that have incorporated amino acid analogs. Within 1 hour after exposure of cells to canavanine, 50% of the analog-containing proteins were degraded to acid-soluble form. At the same time, no net loss of canavanine-containing protein occurred from the 100,000 X g supernatant. Instead, most of the proteins containing the analog, unlike normal ones, accumulated in particulate fractions sedimenting at 10,000 X g or 100,000 X g. They were then lost from these fractions concomitant with the degradation of the abnormal proteins. The loss of such proteins from particulate fractions accounted for all of the protein degraded to acid-soluble form. Similar observations were obtained after incorporation of other analogs or puromycin. The 10,000 X g pellets correspond to amorphous dense intracellular granules visible in electron micrographs of cells exposed to canavanine. Upon removal of the analog, these granules disappeared, simultaneously with the degradation of the analog-containing proteins. These pellets do not resemble a degradative organelle, like the lysosome; they are not osmotically sensitive, do not exclude inulin, are not enclosed by a membrane, and do not show autolytic activity. The proteins in the granules could be solubilized by sodium dodecyl sulfate but not by Triton, NaC1, dithiothreitol, RNase, DNase, or phospholipase. The proteins extracted from the pellet with sodium dodecyl sulfate tend to become particulate again upon removal of this detergent. Incorporation of canavanine caused a normally soluble polypeptide, the monomer of beta-galactosidase, to be inactive and found in the sedimentable fraction. These findings suggest that (a) the presence of amino acid analogs in proteins can make them less soluble, and (b) the inclusions are formed by the spontaneous precipitation of abnormal proteins rather than by an active granule-forming process."}
{"id": "PMID:1089652", "title": "Identification of a ribosomal protein necessary for thiostrepton binding to Escherichia coli ribosomes.", "content": "A ribosomal protein necessary for thiostrepton binding to Escherichia coli ribosomes has been identified using the following criteria: 1. A loss in the thiostrepton binding ability of the ribosome was correlated with the selective removal of ribosomal protein L11. This was achieved by a comparison of the thiostrepton binding ability of 50 S ribosomal subunits treated with 1 M NH4C1 and 50% ethanol at 37 degrees which still contained protein L11, and subunits treated successively at 0 degrees and 37 degrees in the same medium, from which protein L11 had been removed. 2. The thiostrepton binding ability of a ribosomal core containing only seven proteins, produced by treatment of 50 S subunits with 4 M LiC1, was fully restored by the rebinding of protein L11, obtained by Sephadex G-100 fractionation of the 1 M LiC1 split protein fraction from 50 S subunits. In addition, treatment of the 1 M LiC1 split protein fraction with an IgG specific for protein L11, uniquely inhibited the restoration of activity. 3. Thiostrepton binding to the 4 M LiC1 core, reconstituted with the 1 M LiC1 split protein fraction, was blocked by treatment with a monovalent antibody fragment (Fab) prepared against protein L11, but not by treatment with antibodies specific for the proteins of the 4 M LiC1 core. We conclude, therefore, that protein L11 is required for the ribosomal binding of thiostrepton.", "contents": "Identification of a ribosomal protein necessary for thiostrepton binding to Escherichia coli ribosomes. A ribosomal protein necessary for thiostrepton binding to Escherichia coli ribosomes has been identified using the following criteria: 1. A loss in the thiostrepton binding ability of the ribosome was correlated with the selective removal of ribosomal protein L11. This was achieved by a comparison of the thiostrepton binding ability of 50 S ribosomal subunits treated with 1 M NH4C1 and 50% ethanol at 37 degrees which still contained protein L11, and subunits treated successively at 0 degrees and 37 degrees in the same medium, from which protein L11 had been removed. 2. The thiostrepton binding ability of a ribosomal core containing only seven proteins, produced by treatment of 50 S subunits with 4 M LiC1, was fully restored by the rebinding of protein L11, obtained by Sephadex G-100 fractionation of the 1 M LiC1 split protein fraction from 50 S subunits. In addition, treatment of the 1 M LiC1 split protein fraction with an IgG specific for protein L11, uniquely inhibited the restoration of activity. 3. Thiostrepton binding to the 4 M LiC1 core, reconstituted with the 1 M LiC1 split protein fraction, was blocked by treatment with a monovalent antibody fragment (Fab) prepared against protein L11, but not by treatment with antibodies specific for the proteins of the 4 M LiC1 core. We conclude, therefore, that protein L11 is required for the ribosomal binding of thiostrepton."}
{"id": "PMID:1089653", "title": "Assembly of ribosomal proteins L7, L10, L11, and L12, on the 50 S subunit of Escherichia coli.", "content": "We have determined the in vitro assembly sequence of ribosomal proteins L7, L10, L11, and L12 on Escherichia coli 50 S subunits by reconstitution experiments with the use of various ribosomal core particles and split protein fractions produced by treatment of 50 S subunits with 1 m NH4Cl and 50% ethanol. Proteins L7, L10, L11, and L12 were removed by a two-step treatment, first at 0 degrees, then at 37 degrees. Small amounts of proteins L1, L5, and L6 were also removed under these conditions. A one-step extraction of 50 S subunits at 0 degrees removed only proteins L7 and L12, while a similar one-step extraction of intact 50 S subunits at 37 degrees removed proteins L7, L12, and L10. Two-dimensional gel electrophoresis of the protein components and measurement of the ribosome-elongation factor G-guanosine diphosphate complex formed with the various reconstituted particles showed that the binding of proteins L7 and L12 is dependent on the binding of protein L10 and in turn, that the binding of protein L10 is dependent on the binding of protein L11.", "contents": "Assembly of ribosomal proteins L7, L10, L11, and L12, on the 50 S subunit of Escherichia coli. We have determined the in vitro assembly sequence of ribosomal proteins L7, L10, L11, and L12 on Escherichia coli 50 S subunits by reconstitution experiments with the use of various ribosomal core particles and split protein fractions produced by treatment of 50 S subunits with 1 m NH4Cl and 50% ethanol. Proteins L7, L10, L11, and L12 were removed by a two-step treatment, first at 0 degrees, then at 37 degrees. Small amounts of proteins L1, L5, and L6 were also removed under these conditions. A one-step extraction of 50 S subunits at 0 degrees removed only proteins L7 and L12, while a similar one-step extraction of intact 50 S subunits at 37 degrees removed proteins L7, L12, and L10. Two-dimensional gel electrophoresis of the protein components and measurement of the ribosome-elongation factor G-guanosine diphosphate complex formed with the various reconstituted particles showed that the binding of proteins L7 and L12 is dependent on the binding of protein L10 and in turn, that the binding of protein L10 is dependent on the binding of protein L11."}
{"id": "PMID:1089654", "title": "Initiation and transcription of a set of transfer ribonucleic acid genes in vitro.", "content": "Using RNA polymerase purified from Escherichia coli, DNA isolated from the bacteriophage T4, and a bacterial supernatant fraction containing the necessary processing enzymes, a set of transfer RNAs can be formed in vitro. To characterize the site or sites of initiation of this tRNA transcription, rifampicin-resistant complexes of RNA polymerase, DNA, and either ATP (UTP and CTP) or GTP (UTP and CTP) were formed, and tRNA was transcribed from these stabilized sites. It is concluded that transcription of the entire set is initiated by ATP. To study the transcription of the tRNAs, the time sequence of the appearance of individual species was determined during synchronous transcription of a preformed RNA polymerase-DNA complex. The appearance of three RNA species is found to be consistent with the sequential transcription of a large polycistronic cluster; the order and distances, inferred from the times of transcription, are as required by the existing gene map. It is concluded that the initiation of tRNA transcription can occur, without accessory factors, with the insertion of ATP at a single or a few closely spaced sites, and that the tRNAs encoded by the bacteriophage T4 are present in a single operon.", "contents": "Initiation and transcription of a set of transfer ribonucleic acid genes in vitro. Using RNA polymerase purified from Escherichia coli, DNA isolated from the bacteriophage T4, and a bacterial supernatant fraction containing the necessary processing enzymes, a set of transfer RNAs can be formed in vitro. To characterize the site or sites of initiation of this tRNA transcription, rifampicin-resistant complexes of RNA polymerase, DNA, and either ATP (UTP and CTP) or GTP (UTP and CTP) were formed, and tRNA was transcribed from these stabilized sites. It is concluded that transcription of the entire set is initiated by ATP. To study the transcription of the tRNAs, the time sequence of the appearance of individual species was determined during synchronous transcription of a preformed RNA polymerase-DNA complex. The appearance of three RNA species is found to be consistent with the sequential transcription of a large polycistronic cluster; the order and distances, inferred from the times of transcription, are as required by the existing gene map. It is concluded that the initiation of tRNA transcription can occur, without accessory factors, with the insertion of ATP at a single or a few closely spaced sites, and that the tRNAs encoded by the bacteriophage T4 are present in a single operon."}
{"id": "PMID:1089655", "title": "Chemical modification of yeast 3-phosphoglycerate kinase.", "content": "Sulfhydryl reagents, as well as mild hydrogen peroxide oxidation, do not inhibit the activity of yeast phosphoglycerate kinase, indicating that the single thiol group and 3 methionine residues present in the enzyme are not essential for activity. Nitration of phosphoglycerate kinase by tetranitromethane inhibits the enzyme by reaction with a single tyrosine residue. Substrates provide partial protection against inactivation by nitration. Circular dichroism spectra indicate that no conformational changes occur upon nitration. However, perturbation of the microenvironment surrounding the aromatic amino acid residues, particularly tyrosine, was observed. The same perturbation was observed on addition of the substrate 3-phosphoglycerate kinase to native phosphoglycerate kinase. The role of lysine in the action of yeast phosphoglycerate kinase has been studied by modification with O-methylisourea, 2-methoxy-5-nitrotropone, and pyridoxal phosphate. Guanidination shows that there are lysines essential for phosphoglycerate kinase; extrapolation to zero activity indicates that there are three essential lysines as judged by nitrotroponylation and three essential lysines when the enzyme is reacted with pyridoxal phosphate. Substrates afford partial protection and extrapolation to total protection indicates that up to three lysines are protected by MgITP and one lysine by 3-phosphoglycerate. Spectrofluorescence and optical rotatory dispersion measurements show that there is no detectable conformational change for the guanidinated phosphoglycerate kinase and that there are slight changes in the spectra suggesting that there may be slight conformational changes for the nitrotroponylated and the pyridoxal phosphate-modified enzymes.", "contents": "Chemical modification of yeast 3-phosphoglycerate kinase. Sulfhydryl reagents, as well as mild hydrogen peroxide oxidation, do not inhibit the activity of yeast phosphoglycerate kinase, indicating that the single thiol group and 3 methionine residues present in the enzyme are not essential for activity. Nitration of phosphoglycerate kinase by tetranitromethane inhibits the enzyme by reaction with a single tyrosine residue. Substrates provide partial protection against inactivation by nitration. Circular dichroism spectra indicate that no conformational changes occur upon nitration. However, perturbation of the microenvironment surrounding the aromatic amino acid residues, particularly tyrosine, was observed. The same perturbation was observed on addition of the substrate 3-phosphoglycerate kinase to native phosphoglycerate kinase. The role of lysine in the action of yeast phosphoglycerate kinase has been studied by modification with O-methylisourea, 2-methoxy-5-nitrotropone, and pyridoxal phosphate. Guanidination shows that there are lysines essential for phosphoglycerate kinase; extrapolation to zero activity indicates that there are three essential lysines as judged by nitrotroponylation and three essential lysines when the enzyme is reacted with pyridoxal phosphate. Substrates afford partial protection and extrapolation to total protection indicates that up to three lysines are protected by MgITP and one lysine by 3-phosphoglycerate. Spectrofluorescence and optical rotatory dispersion measurements show that there is no detectable conformational change for the guanidinated phosphoglycerate kinase and that there are slight changes in the spectra suggesting that there may be slight conformational changes for the nitrotroponylated and the pyridoxal phosphate-modified enzymes."}
{"id": "PMID:1089656", "title": "Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein.", "content": "Fluorescent beta-galactosides (1-(N-dansyl)amino-beta-D-galactopyranoside (DG0), 2'-(N-dansyl)aminoethyl-beta-D-thiogalactopyranoside (DG2), 2'-(N-dansyl)aminoethyl-beta-D-galactopyranoside (oxy-DG2), and 6'-(N-dansyl)aminohexyl-beta-D-thiogalactopyranoside (DG6)) competitively inhibit lactose transport by membrane vesicles from Escherichia coli ML 309-225, but are not actively transported. An increase in the fluorescence of these dansylgalactosides is observed upon addition of D-lactate, imposition of a membrane diffusion potential (positive outside), or dilution-induced, carrier-mediated lactose efflux. The increase is not observed with 2'-(N-dansyl)aminoethyl-beta-D-thioglucopyranoside nor with membrane vesicles lacking the beta-galactoside transport system. Moreover, the D-lactate-induced fluorescence increase is blocked or rapidly reversed by addition of beta-galactosides, sulfhydryl reagents, inhibitors of D-lactate oxidation, or uncoupling agents. The fluorescence increase exhibits an emission maximum at 500 nm and excitation maxima at 345 nm and at 292 nm. The latter excitation maximum is absent unless D-lactate is added, indicating that the bound dansylgalactoside molecules are excited by energy transfer from the membrane proteins. Titration of vesicles with dansylgalactosides in the presence of D-lactate demonstrates that the lac carrier protein constitutes 3 to 4% oof the total membrane protein, and that the affinity of the carrier for substrate is directly related to the length of the alkyl chain between the galactosidic and the dansyl moieties of the dansylgalactosides. In addition, there is excellent agreement between the affinity constants of the various dansylgalactosides as determined by fluorimetric titration and their apparent Kis for lactose transport (KDs and/or apparent Kis are approximately 550, 3o, 40, and 5 muM FOR DG0, DG2, oxy-DG2, and DG6, respectively). Polarization of fluorescence measurements with DG2 and DG6 demonstrate a dramatic increase in polarization on addition of D-lactate which is reversed by addition of lactose or anaerobiosis. These findings provide strong evidence for the contention that the fluorescence changes observed on \"energization\" of the membrane are due to binding of the dansylgalactosides per se, rather than binding followed by transfer into the hydrophobic interior of the membrane", "contents": "Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein. Fluorescent beta-galactosides (1-(N-dansyl)amino-beta-D-galactopyranoside (DG0), 2'-(N-dansyl)aminoethyl-beta-D-thiogalactopyranoside (DG2), 2'-(N-dansyl)aminoethyl-beta-D-galactopyranoside (oxy-DG2), and 6'-(N-dansyl)aminohexyl-beta-D-thiogalactopyranoside (DG6)) competitively inhibit lactose transport by membrane vesicles from Escherichia coli ML 309-225, but are not actively transported. An increase in the fluorescence of these dansylgalactosides is observed upon addition of D-lactate, imposition of a membrane diffusion potential (positive outside), or dilution-induced, carrier-mediated lactose efflux. The increase is not observed with 2'-(N-dansyl)aminoethyl-beta-D-thioglucopyranoside nor with membrane vesicles lacking the beta-galactoside transport system. Moreover, the D-lactate-induced fluorescence increase is blocked or rapidly reversed by addition of beta-galactosides, sulfhydryl reagents, inhibitors of D-lactate oxidation, or uncoupling agents. The fluorescence increase exhibits an emission maximum at 500 nm and excitation maxima at 345 nm and at 292 nm. The latter excitation maximum is absent unless D-lactate is added, indicating that the bound dansylgalactoside molecules are excited by energy transfer from the membrane proteins. Titration of vesicles with dansylgalactosides in the presence of D-lactate demonstrates that the lac carrier protein constitutes 3 to 4% oof the total membrane protein, and that the affinity of the carrier for substrate is directly related to the length of the alkyl chain between the galactosidic and the dansyl moieties of the dansylgalactosides. In addition, there is excellent agreement between the affinity constants of the various dansylgalactosides as determined by fluorimetric titration and their apparent Kis for lactose transport (KDs and/or apparent Kis are approximately 550, 3o, 40, and 5 muM FOR DG0, DG2, oxy-DG2, and DG6, respectively). Polarization of fluorescence measurements with DG2 and DG6 demonstrate a dramatic increase in polarization on addition of D-lactate which is reversed by addition of lactose or anaerobiosis. These findings provide strong evidence for the contention that the fluorescence changes observed on \"energization\" of the membrane are due to binding of the dansylgalactosides per se, rather than binding followed by transfer into the hydrophobic interior of the membrane"}
{"id": "PMID:1089657", "title": "Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with 2-nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside.", "content": "2-Nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside (azidophenylgalactoside) is a competitive inhibitor of lactose transport in membrane vesicles isolated from Escherichia coli ML 308-225, exhibiting an apparent Ki of 75 muM. The initial rate and steady state level of [3H]azidophenylgalactoside accumulation are markedly stimulated by the addition of D-lactate to vesicles containing the lac transport system, and kinetic studies reveal an apparent Km of 75 muM. Membrane vesicles devoid of the lac transport system do not take up significant amounts of azidophenylgalactoside in the presence or absence of D-lactate. When exposed to visible light in the presence of D-lactate, azidophenylgalactoside irreversibly inactivates the lac transport system. Strikingly, photolytic inactivation is not observed in the absence of D-lactate. Kinetic studies of the inactivation process yield a KD of 77 muM. Since lactose protects against inactivation and azidophenylgalactoside does not inactivate amino acid transport, it is apparent that these effects are specific for the lac transport system. The results are consistent with the proposal that the lac carrier protein is inaccessible to substrate in the absence of energy coupling.", "contents": "Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with 2-nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside. 2-Nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside (azidophenylgalactoside) is a competitive inhibitor of lactose transport in membrane vesicles isolated from Escherichia coli ML 308-225, exhibiting an apparent Ki of 75 muM. The initial rate and steady state level of [3H]azidophenylgalactoside accumulation are markedly stimulated by the addition of D-lactate to vesicles containing the lac transport system, and kinetic studies reveal an apparent Km of 75 muM. Membrane vesicles devoid of the lac transport system do not take up significant amounts of azidophenylgalactoside in the presence or absence of D-lactate. When exposed to visible light in the presence of D-lactate, azidophenylgalactoside irreversibly inactivates the lac transport system. Strikingly, photolytic inactivation is not observed in the absence of D-lactate. Kinetic studies of the inactivation process yield a KD of 77 muM. Since lactose protects against inactivation and azidophenylgalactoside does not inactivate amino acid transport, it is apparent that these effects are specific for the lac transport system. The results are consistent with the proposal that the lac carrier protein is inaccessible to substrate in the absence of energy coupling."}
{"id": "PMID:1089658", "title": "Accumulation of lipid-soluble ions and of rubidium as indicators of the electrical potential in membrane vesicles of Escherichia coli.", "content": "We have studied the accumulation of dibenzyldimethyl-ammonium ion (DDA+) by respiring membrane vesicles of Escherichia coli, as an index of the generation of an electrical gradient during respiration. Nonrespiring vesicles accumulated DDA+ when K+ efflux was induced by valinomycin or monactin. By various criteria this was shown to be the exchange of one cation for another, independent of metabolism and coupled entirely by electrical forces. Uptake of DDA+ by respiring vesicles was inhibited by ionophores that translocate electrical charge and by reagents that block the respiratory chain. Oxamate and p-chloromercuribenzoate inhibited accumulation of DDA+ but did not dissipate a preformed pool; the reason appears to be that these reagents are less inhibitory to transport after lactate oxidation has begun than they are in resting vesicles. Uptake does not appear to involve a biological carrier, but requires trace amounts of a lipid-soluble anion such as tetraphenylboron, which has a catalytic role in DDA+ translocation. Respiring K+ vesicles accumulated substantially less DDA+ than did Na+ vesicles. Na+ was expelled from the vesicles concurrently with DDA+ uptake, whereas Rb+ and K+ were not. Thus, DDA+ uptake, whereas Rb+ and K+ were not. Thus, DDA+ uptake may be limited in the latter case by the availability of anionic groups. This explanation was supported by the finding that the addition of nigericin doubled the capacity of K+ vesicles to take up DDA+, presumably by providing a route for K+ to exit in exchange for H+. Parallel experiments on the valinomycin-dependent accumulation of Rb+ by respiring vesicles indicate that this process is analogous to the uptake of DDA+. Ionophores that elicit electrogenic K+ movement also induced respiration-linked transport. Proton-conducting ionophores and several inhibitors of respiration block Rb+ uptake and dissipated a preformed gradient. Preincubation of the vesicles with oxamate or p-chloromercuribenzoate inhibited Rb+ uptake, but their addition to respiring vesicles again did not cause efflux. Rb+ and DDA+ complete for uptake when present simultaneously. We conclude that the accumulation of both DDA+ and Rb+ occurs in response to an electrical gradient, vesicle interior negative, produced by respiration.", "contents": "Accumulation of lipid-soluble ions and of rubidium as indicators of the electrical potential in membrane vesicles of Escherichia coli. We have studied the accumulation of dibenzyldimethyl-ammonium ion (DDA+) by respiring membrane vesicles of Escherichia coli, as an index of the generation of an electrical gradient during respiration. Nonrespiring vesicles accumulated DDA+ when K+ efflux was induced by valinomycin or monactin. By various criteria this was shown to be the exchange of one cation for another, independent of metabolism and coupled entirely by electrical forces. Uptake of DDA+ by respiring vesicles was inhibited by ionophores that translocate electrical charge and by reagents that block the respiratory chain. Oxamate and p-chloromercuribenzoate inhibited accumulation of DDA+ but did not dissipate a preformed pool; the reason appears to be that these reagents are less inhibitory to transport after lactate oxidation has begun than they are in resting vesicles. Uptake does not appear to involve a biological carrier, but requires trace amounts of a lipid-soluble anion such as tetraphenylboron, which has a catalytic role in DDA+ translocation. Respiring K+ vesicles accumulated substantially less DDA+ than did Na+ vesicles. Na+ was expelled from the vesicles concurrently with DDA+ uptake, whereas Rb+ and K+ were not. Thus, DDA+ uptake, whereas Rb+ and K+ were not. Thus, DDA+ uptake may be limited in the latter case by the availability of anionic groups. This explanation was supported by the finding that the addition of nigericin doubled the capacity of K+ vesicles to take up DDA+, presumably by providing a route for K+ to exit in exchange for H+. Parallel experiments on the valinomycin-dependent accumulation of Rb+ by respiring vesicles indicate that this process is analogous to the uptake of DDA+. Ionophores that elicit electrogenic K+ movement also induced respiration-linked transport. Proton-conducting ionophores and several inhibitors of respiration block Rb+ uptake and dissipated a preformed gradient. Preincubation of the vesicles with oxamate or p-chloromercuribenzoate inhibited Rb+ uptake, but their addition to respiring vesicles again did not cause efflux. Rb+ and DDA+ complete for uptake when present simultaneously. We conclude that the accumulation of both DDA+ and Rb+ occurs in response to an electrical gradient, vesicle interior negative, produced by respiration."}
{"id": "PMID:1089659", "title": "Partial purification and properties of an endoribonuclease isolated from human KB cells.", "content": "With the use of a precursor to Escherichia coli tRNA-Tyr as a substrate, we have detected and partially purified a novel endoribonuclease from the cytoplasm of human KB tissue culture cells. This activity, which we have called RNase NU, cleaves the tRNA precursor at two sites in that part of the molecule which is not included in the mature tRNA sequence and which is normally degraded in vivo. In keeping with this observation, we have found that, of a variety of substrates tested, only those which are unstable in vivo are attacked by RNase NU. RNase NU can be purified from the 0.2 M NH4Cl wash of ribosomes followed by ammonium sulfate fractionation and DEAE-Sephadex chromatography. RNase NU cleaves RNA to create 3'-phosphate-terminated oligonucleotides. It has a pH optimum near 8.0, requires either a monovalent cation (NH4+ is most efficient) or Ca-2+ for optimal activity, and is inhibited by 0.1 M PO4-3-. In the course of purifying RNase NU we have detected and studied the intracellular distribution of other ribonuclease activities in human KB cells.", "contents": "Partial purification and properties of an endoribonuclease isolated from human KB cells. With the use of a precursor to Escherichia coli tRNA-Tyr as a substrate, we have detected and partially purified a novel endoribonuclease from the cytoplasm of human KB tissue culture cells. This activity, which we have called RNase NU, cleaves the tRNA precursor at two sites in that part of the molecule which is not included in the mature tRNA sequence and which is normally degraded in vivo. In keeping with this observation, we have found that, of a variety of substrates tested, only those which are unstable in vivo are attacked by RNase NU. RNase NU can be purified from the 0.2 M NH4Cl wash of ribosomes followed by ammonium sulfate fractionation and DEAE-Sephadex chromatography. RNase NU cleaves RNA to create 3'-phosphate-terminated oligonucleotides. It has a pH optimum near 8.0, requires either a monovalent cation (NH4+ is most efficient) or Ca-2+ for optimal activity, and is inhibited by 0.1 M PO4-3-. In the course of purifying RNase NU we have detected and studied the intracellular distribution of other ribonuclease activities in human KB cells."}
{"id": "PMID:1089660", "title": "Characterization of ribonuclease NU cleavage sites in a bacteriophage phi80-induced ribonucleic acid.", "content": "Ribonuclease NU, an endoribonuclease isolated from human KB tissue culture cells, can cleave a bacteriophage phi80-induced RNA at four distinct sites. Nucleotide sequence analysis of the eight cleavage products has shown that the enzyme produces oligonucleotides terminating in 3'-phosphate groups, and that the four cleavage sites are in the only nonhydrogen-bonded region of the substrate. Various aspects of the cleavage reaction with this RNA and with other substrates are discussed.", "contents": "Characterization of ribonuclease NU cleavage sites in a bacteriophage phi80-induced ribonucleic acid. Ribonuclease NU, an endoribonuclease isolated from human KB tissue culture cells, can cleave a bacteriophage phi80-induced RNA at four distinct sites. Nucleotide sequence analysis of the eight cleavage products has shown that the enzyme produces oligonucleotides terminating in 3'-phosphate groups, and that the four cleavage sites are in the only nonhydrogen-bonded region of the substrate. Various aspects of the cleavage reaction with this RNA and with other substrates are discussed."}
{"id": "PMID:1089661", "title": "Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.", "content": "The DNA-directed in vitro synthesis of beta-galactosidase has been investigated in a system dependent on Escherichia coli ribosomes, a salt wash of the ribosomes, and a supernatant fraction. Fractionation of the supernatant has made it possible to obtain dependencies on RNA polymerase and another protein factor for beta-galactosidase synthesis. The other factor (called L factor) cannot be replaced by a variety of proteins known to be required for transcription and translation. It has been purified to homogeneity and has a molecular weight of approximately 65,000. Although it is required for the in vitro synthesis of beta-galactosidase, it has no effect on total DNA-dependent amino acid incorporation under the conditions of the incubation. However, total RNA synthesis is depressed by the addition of L factor in a manner similar to what is observed with rho factor could not replace L factor in beta-galactosidase synthesis.", "contents": "Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase. The DNA-directed in vitro synthesis of beta-galactosidase has been investigated in a system dependent on Escherichia coli ribosomes, a salt wash of the ribosomes, and a supernatant fraction. Fractionation of the supernatant has made it possible to obtain dependencies on RNA polymerase and another protein factor for beta-galactosidase synthesis. The other factor (called L factor) cannot be replaced by a variety of proteins known to be required for transcription and translation. It has been purified to homogeneity and has a molecular weight of approximately 65,000. Although it is required for the in vitro synthesis of beta-galactosidase, it has no effect on total DNA-dependent amino acid incorporation under the conditions of the incubation. However, total RNA synthesis is depressed by the addition of L factor in a manner similar to what is observed with rho factor could not replace L factor in beta-galactosidase synthesis."}
{"id": "PMID:1089662", "title": "Threonine deaminase from Salmonella typhimurium. Relationship between regulatory sites.", "content": "Kinetic analysis of the biosynthetic threonine deaminase, EC 4.2.1.16, from Samonella typhimurium yields hyperbolic substrate saturation curves in the absence of, and higher order substrate saturation curves in the presence of, L-isoleucine. L-Valine reverses this effect of L-isoleucine by restoring the hyperbolic substrate saturation curves. The inhibition of enzyme activity and the reversal of valine stimulation is a function of a second order concentration of L-isoleucine, whereas antagonism of inhibition is a function of first order concentration of valine. The antagonistic effects on enzyme activity of L-isoleucine and of L-valine appear as competitive in diagnostic plots. Threonine deaminase possesses two L-isoleucine binding sites (Kd equals 3.6 muM) and one L-valine binding site (Kd equals 26 muM); the binding of these ligands appear competitive. Exclusion of L-valine requires the binding of 2 molecules of L-isoleucine whereas binding of a single L-valine molecule prevents the binding of 2 L-isoleucine molecules. Cooperative binding of L-isoleucine is not observed under any of the conditions tested. Two cases, expressed in terms of modified Adair equations and based upon the assumption that L-threonine also serves as an activator ligand which binds to the L-valine site, are presented. Case I states that liganding of the activator sites must percede substrate-binding at the active site, and Case II states that the activator site liganding is required solely for reactivation of the L-isoleucine-inhibited enzyme. Analysis of kinetic data by a curve-fitting process suggests that Case II described the relationship between the activator site and the L-isoleucine sites. An enzymatically inactive derivative of threonine deaminase, prepared by reduction with borohydride, binds isoleucine and valine in a manner similar to native holoenzyme. Binding of L-threonine and L-valine to the derivatized enzyme is competitive. The Kd for threonine binding is 3 mM, which is in excellent agreement with the Kd determined by the curve fitting process. It is concluded that the modulation of threonine deaminase activity is wrought by interaction between inhibitor sites and an activator site rather than inhibitor and active sites and that induced transitions rather than concerted transitions more adequately describe the underlying regulatory principle.", "contents": "Threonine deaminase from Salmonella typhimurium. Relationship between regulatory sites. Kinetic analysis of the biosynthetic threonine deaminase, EC 4.2.1.16, from Samonella typhimurium yields hyperbolic substrate saturation curves in the absence of, and higher order substrate saturation curves in the presence of, L-isoleucine. L-Valine reverses this effect of L-isoleucine by restoring the hyperbolic substrate saturation curves. The inhibition of enzyme activity and the reversal of valine stimulation is a function of a second order concentration of L-isoleucine, whereas antagonism of inhibition is a function of first order concentration of valine. The antagonistic effects on enzyme activity of L-isoleucine and of L-valine appear as competitive in diagnostic plots. Threonine deaminase possesses two L-isoleucine binding sites (Kd equals 3.6 muM) and one L-valine binding site (Kd equals 26 muM); the binding of these ligands appear competitive. Exclusion of L-valine requires the binding of 2 molecules of L-isoleucine whereas binding of a single L-valine molecule prevents the binding of 2 L-isoleucine molecules. Cooperative binding of L-isoleucine is not observed under any of the conditions tested. Two cases, expressed in terms of modified Adair equations and based upon the assumption that L-threonine also serves as an activator ligand which binds to the L-valine site, are presented. Case I states that liganding of the activator sites must percede substrate-binding at the active site, and Case II states that the activator site liganding is required solely for reactivation of the L-isoleucine-inhibited enzyme. Analysis of kinetic data by a curve-fitting process suggests that Case II described the relationship between the activator site and the L-isoleucine sites. An enzymatically inactive derivative of threonine deaminase, prepared by reduction with borohydride, binds isoleucine and valine in a manner similar to native holoenzyme. Binding of L-threonine and L-valine to the derivatized enzyme is competitive. The Kd for threonine binding is 3 mM, which is in excellent agreement with the Kd determined by the curve fitting process. It is concluded that the modulation of threonine deaminase activity is wrought by interaction between inhibitor sites and an activator site rather than inhibitor and active sites and that induced transitions rather than concerted transitions more adequately describe the underlying regulatory principle."}
{"id": "PMID:1089663", "title": "Investigations concerning the mode of action of 3,4-dihydroxybutyl-1-phosphonate on Escherichia coli.", "content": "Experiments were performed to evaluate the ability of the enzymes of Escherichia coli involved in glycerol 3-phosphate metabolism to recognize phosphonic acid analogues of the natural substrate. Neither the catabolic membrane-bound glycerol-3-phosphate dehydrogenase nor the acyl coenzyme A: glycerol-3-phosphate acyltransferase can use 3,4-dihydroxybutyl-1-phosphnate or 2,3-dihydroxypropyl-1-phosphonate are inhibitors of the reduction of dihydroxyactone phosphate as substrates. The 4-carbon phosphonic acid analogue does not exhibit inhibitory activity for either of these enzymes. While the 3-carbon phosphonic acid analogue has no inhibitory effect upon the catabolic dehydrogenase, it does appear to have a slight but reproducible inhibitory effect on the acyltransferase. Glycerol 3-phosphate and 3,4-dihydroxybutyl-1-phosphonate by glycerol 3-phosphate:NAD (P) oxidoreductase. rac-2,3-Dihydroxypropyl-1-phosphonate does not appear to be recognized by this enzyme. The apparent K-i for snglycerol 3-phosphate is 19 muM and for D-3,4-dihydroxybutyl-1-phosphonate it is 42 muM. In addition the glycerol 3-phosphate:NAD(P) oxidoreductase catalyzes the reduction of 4-hydroxy-3-oxobutyl-1-phosphonate (apparent K-m of 182 muM), a phosphonic acid analogue of dihydroxyacetone phosphate. 3,4-Dihydroxybutyl-1-phosphonate is both a competitive inhibitor (apparent Ki of 740 muM) and a substrate (apparent K-m of 450 muM) for the CDP-diglyceride: glycerol 3 phosphate phosphatidyltransferase but it has no effect upon CDP-diglyceride:L-serine phosphatidyltransferase. The relationship ofthese in vitro studies to in vivo investigations is discussed.", "contents": "Investigations concerning the mode of action of 3,4-dihydroxybutyl-1-phosphonate on Escherichia coli. Experiments were performed to evaluate the ability of the enzymes of Escherichia coli involved in glycerol 3-phosphate metabolism to recognize phosphonic acid analogues of the natural substrate. Neither the catabolic membrane-bound glycerol-3-phosphate dehydrogenase nor the acyl coenzyme A: glycerol-3-phosphate acyltransferase can use 3,4-dihydroxybutyl-1-phosphnate or 2,3-dihydroxypropyl-1-phosphonate are inhibitors of the reduction of dihydroxyactone phosphate as substrates. The 4-carbon phosphonic acid analogue does not exhibit inhibitory activity for either of these enzymes. While the 3-carbon phosphonic acid analogue has no inhibitory effect upon the catabolic dehydrogenase, it does appear to have a slight but reproducible inhibitory effect on the acyltransferase. Glycerol 3-phosphate and 3,4-dihydroxybutyl-1-phosphonate by glycerol 3-phosphate:NAD (P) oxidoreductase. rac-2,3-Dihydroxypropyl-1-phosphonate does not appear to be recognized by this enzyme. The apparent K-i for snglycerol 3-phosphate is 19 muM and for D-3,4-dihydroxybutyl-1-phosphonate it is 42 muM. In addition the glycerol 3-phosphate:NAD(P) oxidoreductase catalyzes the reduction of 4-hydroxy-3-oxobutyl-1-phosphonate (apparent K-m of 182 muM), a phosphonic acid analogue of dihydroxyacetone phosphate. 3,4-Dihydroxybutyl-1-phosphonate is both a competitive inhibitor (apparent Ki of 740 muM) and a substrate (apparent K-m of 450 muM) for the CDP-diglyceride: glycerol 3 phosphate phosphatidyltransferase but it has no effect upon CDP-diglyceride:L-serine phosphatidyltransferase. The relationship ofthese in vitro studies to in vivo investigations is discussed."}
{"id": "PMID:1089664", "title": "Studies on tyrosine phenol lyase. Modification of essential histidyl residues by diethylpyrocarbonate.", "content": "Tyrosine phenol-lyase of Escherichia intermedia is inactivated by treatment with diethylpyrocarbonate at pH 6.0 AND 4 degrees. Spectrophotometric studies show that the inactivation is stoichiometric, with a modification of 2 histidyl residues per molecule of the enzyme. Finding that this inactivation is largely reversed by treatment with hydroxylamine indicates that the inactivation is mainly due to modification of the histidyl residues. No changes in the sulfhydryl content or in the aromatic amino acids are observed as a result of this modification. The modified tyrosine phenol-lyase retains most of its ability to form a nearly normal complex with its coenzyme, pyridoxal phosphate. This has been shown by studies of its absorption, by the determination of pyridoxal phosphate, and by reduction of the holoenzyme with tritiated sodium borohydride. The modified enzyme also appears to form a Schiff base intermediate with L-alanine. The modified holoenzyme fails to catalyze the exchange of the alpha-hydrogen of L-alanine with tritium from tritiated water. This is consistent with a catalytic role for modified histidyl residues at the active site of the enzyme; this role is the removal of the alpha-hydrogen of substrates.", "contents": "Studies on tyrosine phenol lyase. Modification of essential histidyl residues by diethylpyrocarbonate. Tyrosine phenol-lyase of Escherichia intermedia is inactivated by treatment with diethylpyrocarbonate at pH 6.0 AND 4 degrees. Spectrophotometric studies show that the inactivation is stoichiometric, with a modification of 2 histidyl residues per molecule of the enzyme. Finding that this inactivation is largely reversed by treatment with hydroxylamine indicates that the inactivation is mainly due to modification of the histidyl residues. No changes in the sulfhydryl content or in the aromatic amino acids are observed as a result of this modification. The modified tyrosine phenol-lyase retains most of its ability to form a nearly normal complex with its coenzyme, pyridoxal phosphate. This has been shown by studies of its absorption, by the determination of pyridoxal phosphate, and by reduction of the holoenzyme with tritiated sodium borohydride. The modified enzyme also appears to form a Schiff base intermediate with L-alanine. The modified holoenzyme fails to catalyze the exchange of the alpha-hydrogen of L-alanine with tritium from tritiated water. This is consistent with a catalytic role for modified histidyl residues at the active site of the enzyme; this role is the removal of the alpha-hydrogen of substrates."}
{"id": "PMID:1089665", "title": "N-Acetylglutamate synthase of Escherichia coli regulation of synthesis and activity by arginine.", "content": "N-Acetylglutamate synthase, the first enzyme of arginine biosynthesis, was stabilized in crude extracts from Escherichia coli. At 4 degrees the enzyme lost less than 5% of activity per day. L-Arginine repressed the formation of N-acetylglutamate synthase. Under conditions of genetic or physiological derepression, a specific activity of approximately 50 nmol per min per mg of protein was measured. No activity (i.e. less than 0.2 nmol per min per mg of protein) could be detected in extracts from cells grown under conditions of repression, whereas an intermediate level was found in cell cultivated on minimal medium. In a 6-fold purified preparation L-arginine inhibited the enzyme. Of 11 precursors and analogues of arginine tested only O-[L-norvalyl-5]-isourea inhibited N-acetylglutamate synthase as strongly as L-agrinine.", "contents": "N-Acetylglutamate synthase of Escherichia coli regulation of synthesis and activity by arginine. N-Acetylglutamate synthase, the first enzyme of arginine biosynthesis, was stabilized in crude extracts from Escherichia coli. At 4 degrees the enzyme lost less than 5% of activity per day. L-Arginine repressed the formation of N-acetylglutamate synthase. Under conditions of genetic or physiological derepression, a specific activity of approximately 50 nmol per min per mg of protein was measured. No activity (i.e. less than 0.2 nmol per min per mg of protein) could be detected in extracts from cells grown under conditions of repression, whereas an intermediate level was found in cell cultivated on minimal medium. In a 6-fold purified preparation L-arginine inhibited the enzyme. Of 11 precursors and analogues of arginine tested only O-[L-norvalyl-5]-isourea inhibited N-acetylglutamate synthase as strongly as L-agrinine."}
{"id": "PMID:1089666", "title": "The fate of ribosomes in Escherichia coli cells starved for a carbon source.", "content": "The disappearance of ribosomes in Escherichia coli cells starved for a carbon source was studied. We used a series of mutants, some of them lacking in ribonuclease I(RNase I, EC 2.7.7.17), and other containing various combinations of modified polynucleotide phosphorylase (PNPase, EC 2.7.7.8) and modified ribonuclease II (RNase II, EC 3.1.4.1). RNA was prepared from the starved mutant cells and separated on polyacrylamide gels. The results obtained indicate that 23 S RNA degradation is similar in all strains that lack RNase I, and is slightly increased in the strain that contains this enzyme. The extent of 16 S RNA degradation is identical in all strains tested. RNA species in the size of 4 S and smaller accumulate in mutants containing modified forms of PNPase and RNase II. The appearance of an RNA species 10% smaller than 16 S RNA (d16 S RNA) was observed in all strains that contain unmodified RNase II. Analysis of ribosomes and polysomes and their RNA content indicated that polysomes are converted to monosomes and these, in turn, to ribosomal subunits. No RNA degradation products were found in polysomes, 70 S, OR 50 C particle; 30 S subunits contained 16 S RNA as well as the d16 S RNA species. Subunits are degraded to a similar extent in all strains lacking RNase I, and at a slightly faster rate in the strain that contains RNase I. The RNA to protein ratio in subunits prepared from starved cells is similar to that of unstarved cultures. Very little degradation of ribosomal proteins occurs in these mutants during carbon starvation. The proteins released from degraded ribosomes are found in the fast sedimenting (20,000 times g) pellet. Cell viability studies indicated a direct correlation between the capacity of the mutants to recovery from starvation and their capacity to degrade RNA. Thus a biological necessity for degradation of ribosomes during starvation is implied. Based on these data we propose that the endonucleolytic degradation of ribosomal RNA is the primary event in starvation degradation. It takes place in ribosomal subunits, which fall apart after the endonucleoltic attack. The RNA pieces produced by this cleavage are degraded to nucleotide by RNase II and PNPase. The ribosomal proteins attach to the cell membrane.", "contents": "The fate of ribosomes in Escherichia coli cells starved for a carbon source. The disappearance of ribosomes in Escherichia coli cells starved for a carbon source was studied. We used a series of mutants, some of them lacking in ribonuclease I(RNase I, EC 2.7.7.17), and other containing various combinations of modified polynucleotide phosphorylase (PNPase, EC 2.7.7.8) and modified ribonuclease II (RNase II, EC 3.1.4.1). RNA was prepared from the starved mutant cells and separated on polyacrylamide gels. The results obtained indicate that 23 S RNA degradation is similar in all strains that lack RNase I, and is slightly increased in the strain that contains this enzyme. The extent of 16 S RNA degradation is identical in all strains tested. RNA species in the size of 4 S and smaller accumulate in mutants containing modified forms of PNPase and RNase II. The appearance of an RNA species 10% smaller than 16 S RNA (d16 S RNA) was observed in all strains that contain unmodified RNase II. Analysis of ribosomes and polysomes and their RNA content indicated that polysomes are converted to monosomes and these, in turn, to ribosomal subunits. No RNA degradation products were found in polysomes, 70 S, OR 50 C particle; 30 S subunits contained 16 S RNA as well as the d16 S RNA species. Subunits are degraded to a similar extent in all strains lacking RNase I, and at a slightly faster rate in the strain that contains RNase I. The RNA to protein ratio in subunits prepared from starved cells is similar to that of unstarved cultures. Very little degradation of ribosomal proteins occurs in these mutants during carbon starvation. The proteins released from degraded ribosomes are found in the fast sedimenting (20,000 times g) pellet. Cell viability studies indicated a direct correlation between the capacity of the mutants to recovery from starvation and their capacity to degrade RNA. Thus a biological necessity for degradation of ribosomes during starvation is implied. Based on these data we propose that the endonucleolytic degradation of ribosomal RNA is the primary event in starvation degradation. It takes place in ribosomal subunits, which fall apart after the endonucleoltic attack. The RNA pieces produced by this cleavage are degraded to nucleotide by RNase II and PNPase. The ribosomal proteins attach to the cell membrane."}
{"id": "PMID:1089667", "title": "A kinetic study of dihydrolipoyl transacetylase from bovine kidney.", "content": "The mammalian pyruvate dehydrogenase complex contains a core, consisting of dihydrolipoyl transacetylase, to which pyruvate dehydrogenase and dihydrolipoyl dehydrogenase are joined. This report describes studies on the kinetic mechanism of the transacetylase-catalyzed reaction between [1-14C]acetyl-CoA and dihydrolipoamide. This reaction appears to be a model of the physiological reaction, in which the acetyl group is transferred from the S-acetyldihydrolipoyl moiety, bound covalently to the transacetylase, to CoA. The model reaction is not affected by pyruvate dehydrogenase or dihydrolipoyl dehydrogenase, their substrates and products, or by removal of the covalently bound lipoyl moiety. These findings, together with the results of initial velocity, product inhibition, and dead-end inhibition studies, indicate that the model reaction and, apparently, the physiological reaction as well, proceeds via the Random Bi Bi (rapid equilibrium) mechanism. It appears that at the catalytic center of the transacetylase there are two adjacent sites, one that binds CoA and acetyl-CoA and another that binds dihydrolipoamide and S-acetyldihydrolipoamide (or the corresponding forms of the covalently bound lipoyl moiety.", "contents": "A kinetic study of dihydrolipoyl transacetylase from bovine kidney. The mammalian pyruvate dehydrogenase complex contains a core, consisting of dihydrolipoyl transacetylase, to which pyruvate dehydrogenase and dihydrolipoyl dehydrogenase are joined. This report describes studies on the kinetic mechanism of the transacetylase-catalyzed reaction between [1-14C]acetyl-CoA and dihydrolipoamide. This reaction appears to be a model of the physiological reaction, in which the acetyl group is transferred from the S-acetyldihydrolipoyl moiety, bound covalently to the transacetylase, to CoA. The model reaction is not affected by pyruvate dehydrogenase or dihydrolipoyl dehydrogenase, their substrates and products, or by removal of the covalently bound lipoyl moiety. These findings, together with the results of initial velocity, product inhibition, and dead-end inhibition studies, indicate that the model reaction and, apparently, the physiological reaction as well, proceeds via the Random Bi Bi (rapid equilibrium) mechanism. It appears that at the catalytic center of the transacetylase there are two adjacent sites, one that binds CoA and acetyl-CoA and another that binds dihydrolipoamide and S-acetyldihydrolipoamide (or the corresponding forms of the covalently bound lipoyl moiety."}
{"id": "PMID:1089668", "title": "Angiography in the diagnosis of osteoid-osteoma of the hand.", "content": "Angiographic findings characteristic of osteoid-osteoma are described in two cases of osteoid-osteoma of the bones of the wrist. While the etiology of the disease is not understood, a developmental relationship between the osteoid-osteoma nidus and increased vascularity is suggested.", "contents": "Angiography in the diagnosis of osteoid-osteoma of the hand. Angiographic findings characteristic of osteoid-osteoma are described in two cases of osteoid-osteoma of the bones of the wrist. While the etiology of the disease is not understood, a developmental relationship between the osteoid-osteoma nidus and increased vascularity is suggested."}
{"id": "PMID:1089669", "title": "Pelvic displacement osteotomy for chronic hip dislocation in myelodysplasia.", "content": "Twelve children with lumbar-level myelodysplasia (average age, eight and three-quarter years) underwent twenty-one pelvic displacement osteotomies for subluxated or dislocated hips. Nineteen of the twenty-one hips remained reduced on three-year follow-up. Gains in gait pattern, ease of bracing, and reduced pelvic obliquity were noted. Active function about the hips was not improved, nor was there a decrease in the amount of bracing needed following the osteotomy. Pelvic displacement osteotomy can be utilized in selected cases as part of the over-all management of chronic hip dislocation in myelodysplasia.", "contents": "Pelvic displacement osteotomy for chronic hip dislocation in myelodysplasia. Twelve children with lumbar-level myelodysplasia (average age, eight and three-quarter years) underwent twenty-one pelvic displacement osteotomies for subluxated or dislocated hips. Nineteen of the twenty-one hips remained reduced on three-year follow-up. Gains in gait pattern, ease of bracing, and reduced pelvic obliquity were noted. Active function about the hips was not improved, nor was there a decrease in the amount of bracing needed following the osteotomy. Pelvic displacement osteotomy can be utilized in selected cases as part of the over-all management of chronic hip dislocation in myelodysplasia."}
{"id": "PMID:1089670", "title": "The immunogenicity of fresh and frozen allogeneic bone.", "content": "Both fresh and frozen allogeneic bone elicit both acellular and humoral immune response. This response includes the development of enhancing factors which block detectable immunity and probably protect the graft from rejection. There seems to be no evidence of an alteration in immunogenicity by freezing of the graft. The importance of these observations lies in the potential technique of employing fresh viable allografts; prior freezing and tissue matching for HL-A transplantation antigens should not be necessary.", "contents": "The immunogenicity of fresh and frozen allogeneic bone. Both fresh and frozen allogeneic bone elicit both acellular and humoral immune response. This response includes the development of enhancing factors which block detectable immunity and probably protect the graft from rejection. There seems to be no evidence of an alteration in immunogenicity by freezing of the graft. The importance of these observations lies in the potential technique of employing fresh viable allografts; prior freezing and tissue matching for HL-A transplantation antigens should not be necessary."}
{"id": "PMID:1089671", "title": "Physical and biological aspects of repair in dog cortical-bone transplants.", "content": "The amount of repair and the time required to accomplish repair of four-centimeter segmental fibular transplants in twenty-one male adult dogs were determined at from two to forty-eight weeks after transplantation by torsional stress testing, microradiography, and tetracycline labeling. The transplanted cortical bone was greatly weakened at from six weeks to six months but was nearly normal at one year. The strength of the transplant appeared to be related to the amount of porosity of the matrix rather than to the quality or completeness of biological repair. Spatially, the repair was ordered rather than random. The initial resorption caused increased porosity which was slowly offset by apposition of new bone, a process which was dependent on general skeletal metabolic activity. Although physical strength was near normal at forty-eight weeks, only 60 per cent of the transplants had been remodeled.", "contents": "Physical and biological aspects of repair in dog cortical-bone transplants. The amount of repair and the time required to accomplish repair of four-centimeter segmental fibular transplants in twenty-one male adult dogs were determined at from two to forty-eight weeks after transplantation by torsional stress testing, microradiography, and tetracycline labeling. The transplanted cortical bone was greatly weakened at from six weeks to six months but was nearly normal at one year. The strength of the transplant appeared to be related to the amount of porosity of the matrix rather than to the quality or completeness of biological repair. Spatially, the repair was ordered rather than random. The initial resorption caused increased porosity which was slowly offset by apposition of new bone, a process which was dependent on general skeletal metabolic activity. Although physical strength was near normal at forty-eight weeks, only 60 per cent of the transplants had been remodeled."}
{"id": "PMID:1089672", "title": "Neglected rupture of the Achilles tendon. Treatment by V-Y tendinous flap.", "content": "A method is described that achieves end-to-end anastomosis of ruptures of the Achilles tendon. A sliding tendinous flap is developed over the proximal portion of the tendon by making an inverted V incision which is then repaired in a Y fashion. The procedure is recommended for patients with neglected ruptures of the Achilles tendon in which end-to-end anastomosis is otherwise impossible.", "contents": "Neglected rupture of the Achilles tendon. Treatment by V-Y tendinous flap. A method is described that achieves end-to-end anastomosis of ruptures of the Achilles tendon. A sliding tendinous flap is developed over the proximal portion of the tendon by making an inverted V incision which is then repaired in a Y fashion. The procedure is recommended for patients with neglected ruptures of the Achilles tendon in which end-to-end anastomosis is otherwise impossible."}
{"id": "PMID:1089673", "title": "The torn shoulder capsule.", "content": "Tears of the shoulder capsule attended by retraction of the edges of the tear, inability to close the gap, and pathological changes in the biceps tendon may be very effectively managed by: (1) a transacromial exposure with a V-shaped osteotomy and subsequent replacement of the acromion with bone screws, and (2) standard repairs of the shoulder capsule after the biceps tendon has been reseated in a newly constructed, more laterally located groove in the head of the humerus. The follow-up results document the value of these procedures done on a group of fourteen patients.", "contents": "The torn shoulder capsule. Tears of the shoulder capsule attended by retraction of the edges of the tear, inability to close the gap, and pathological changes in the biceps tendon may be very effectively managed by: (1) a transacromial exposure with a V-shaped osteotomy and subsequent replacement of the acromion with bone screws, and (2) standard repairs of the shoulder capsule after the biceps tendon has been reseated in a newly constructed, more laterally located groove in the head of the humerus. The follow-up results document the value of these procedures done on a group of fourteen patients."}
{"id": "PMID:1089675", "title": "Observations on the male nucleus during fertilization in the fern Pteridium aquilinum.", "content": "Fertilization of eggs of Pteridium under controlled conditions has revealed that archegonia containing viable eggs require more than 30 min flooding before opening. Syngamy appears to take place by an engulfing action, and no evidence was found of a 'membrane-fusion' kind of conjugation. Although the gametic nucleus lacks a recognizable envelope, as soon as it comes into contact with the female cytoplasm an envelope is reconstituted from an opaque layer at the surface of its chromatin. This membrane also undergoes growth, the outer layer extending into the cytoplasm and surrounding spermatozoid mitochondria. The male envelope remains distinct from the female, and lacks evaginations and well defined pores. The male nucleus lies in irregular loops within a funnel, filled with egg cytoplasm, formed by the microtubular ribbon of the gamete. The mouth of the funnel is bordered by the multi-layered structure. Karyogamy is initiated at the anterior end of the male nucleus, the orifice connecting the 2 nuclei widening from about 0-1 to 2 mum in diameter during fusion. The contents of the male nucleus flow through the mouth of the funnel into the female, the chromatin relaxing locally, but retaining a rod-like form. The envelope of the male nucleus is probably resorbed at the site of junction. There is no evidence that it contributes significantly to the envelope of the zygotic nucleus.", "contents": "Observations on the male nucleus during fertilization in the fern Pteridium aquilinum. Fertilization of eggs of Pteridium under controlled conditions has revealed that archegonia containing viable eggs require more than 30 min flooding before opening. Syngamy appears to take place by an engulfing action, and no evidence was found of a 'membrane-fusion' kind of conjugation. Although the gametic nucleus lacks a recognizable envelope, as soon as it comes into contact with the female cytoplasm an envelope is reconstituted from an opaque layer at the surface of its chromatin. This membrane also undergoes growth, the outer layer extending into the cytoplasm and surrounding spermatozoid mitochondria. The male envelope remains distinct from the female, and lacks evaginations and well defined pores. The male nucleus lies in irregular loops within a funnel, filled with egg cytoplasm, formed by the microtubular ribbon of the gamete. The mouth of the funnel is bordered by the multi-layered structure. Karyogamy is initiated at the anterior end of the male nucleus, the orifice connecting the 2 nuclei widening from about 0-1 to 2 mum in diameter during fusion. The contents of the male nucleus flow through the mouth of the funnel into the female, the chromatin relaxing locally, but retaining a rod-like form. The envelope of the male nucleus is probably resorbed at the site of junction. There is no evidence that it contributes significantly to the envelope of the zygotic nucleus."}
{"id": "PMID:1089676", "title": "Observations on the ultrastructure of the choanoflagellate Codosiga botrytis (Ehr.) Saville-Kent with special reference to the flagellar apparatus.", "content": "The ultrastructure of the choanoflagellate Codosiga botrytis is described with particular reference to the flagellar appendages, the flagellar rootlet system, the transition zone, the basal body and accessory centrioles, and the stalk. The controversial early reports of flagellar appendages in this species have been confirmed and they have been detected in 2 further species, Salpingoeca frequentissima and Monosiga sp. The appendages consist of a delicate bilateral vane 2 mum wide on either side of the axis, composed of extremely fine overlapping or interwoven fibrils. The flagellar root system consists of a large number of radiating microtubules associated with bands of electron-dense material near the basal body; striated roots are absent. The microtubules extend from several separate foci, those in any one group originating near a composite electron-dense band, and for a distance of 300 nm from the basal body they are separated by blocks of interstitial material. The flagellar basal body forms one of a diplosome pair of centrioles. The triplet microtubules of the accessory centriole are embedded in amorphous electron-dense material and the whole is enveloped in a sheath of similar appearance. The existence of a third centriole close to the diplosome pair is also reported. The relatively complex structure of the flagellar transitional zone is described. The stalk is composed of a core of circular lacunae, which may or may not contain finger-like protoplasmic extensions of the posterior end of the cell, surrounded by a continuation of the sheath material which encloses the remainder of the protoplast. In the stalk only there is a further closely sheathing layer about 15 nm thick which is regularly striated, the spacing of the striations in shadowcast material and sections being about 3 times that measured by negative staining. The structure of choanoflagellates differs widely from that of the algal class Chrysophyceae, the group in which they are included in some classifications, and from the remainder of the algae; they do not appear to have a place in either the algae or the plant kingdom. The structure of Codosiga botrytis is briefly compared with that of sponge choanocytes and collared cells in the Metazoa and some of the possible phylogenetic implications of this are indicated.", "contents": "Observations on the ultrastructure of the choanoflagellate Codosiga botrytis (Ehr.) Saville-Kent with special reference to the flagellar apparatus. The ultrastructure of the choanoflagellate Codosiga botrytis is described with particular reference to the flagellar appendages, the flagellar rootlet system, the transition zone, the basal body and accessory centrioles, and the stalk. The controversial early reports of flagellar appendages in this species have been confirmed and they have been detected in 2 further species, Salpingoeca frequentissima and Monosiga sp. The appendages consist of a delicate bilateral vane 2 mum wide on either side of the axis, composed of extremely fine overlapping or interwoven fibrils. The flagellar root system consists of a large number of radiating microtubules associated with bands of electron-dense material near the basal body; striated roots are absent. The microtubules extend from several separate foci, those in any one group originating near a composite electron-dense band, and for a distance of 300 nm from the basal body they are separated by blocks of interstitial material. The flagellar basal body forms one of a diplosome pair of centrioles. The triplet microtubules of the accessory centriole are embedded in amorphous electron-dense material and the whole is enveloped in a sheath of similar appearance. The existence of a third centriole close to the diplosome pair is also reported. The relatively complex structure of the flagellar transitional zone is described. The stalk is composed of a core of circular lacunae, which may or may not contain finger-like protoplasmic extensions of the posterior end of the cell, surrounded by a continuation of the sheath material which encloses the remainder of the protoplast. In the stalk only there is a further closely sheathing layer about 15 nm thick which is regularly striated, the spacing of the striations in shadowcast material and sections being about 3 times that measured by negative staining. The structure of choanoflagellates differs widely from that of the algal class Chrysophyceae, the group in which they are included in some classifications, and from the remainder of the algae; they do not appear to have a place in either the algae or the plant kingdom. The structure of Codosiga botrytis is briefly compared with that of sponge choanocytes and collared cells in the Metazoa and some of the possible phylogenetic implications of this are indicated."}
{"id": "PMID:1089681", "title": "Hygiene in the prevention of udder infections. I. Comparative efficacy of four teat dips.", "content": "Three trials of teat dips were conducted with experimental exposure to mastitis pathogens. A control group of animals and a group treated with a hypochlorite preparation were included in all trials while a third group was dipped with a different iodophor preparation in each trial. Four teat dips were highly effective in reducing the incidence of udder infection. Some teat irritation was transitory during each trial; most occurred in the hypochlorite group.", "contents": "Hygiene in the prevention of udder infections. I. Comparative efficacy of four teat dips. Three trials of teat dips were conducted with experimental exposure to mastitis pathogens. A control group of animals and a group treated with a hypochlorite preparation were included in all trials while a third group was dipped with a different iodophor preparation in each trial. Four teat dips were highly effective in reducing the incidence of udder infection. Some teat irritation was transitory during each trial; most occurred in the hypochlorite group."}
{"id": "PMID:1089682", "title": "Hygiene in the prevention of udder infections. II. Evaluation of oil-based teat dips.", "content": "Oil-based teat dips containing iodine at .5 and 1% were compared in a series of studies with two water-based iodophor products of the same concentration and also with undipped controls. When the two types of products were tested at .5% under conditions of natural infection, the water-based dip was four times more effective than the oil-based dip at preventing Staphylococcus aureus infections. In three subsequent trials teats were experimentally exposed to S. aureus and Streptococcus agalactiae. Five times more S. aureus infections developed in a group dipped with the oil-based dip at 1% iodine with an undipped control yielded no significant difference for S. aureus infections. The 1% iodine water-based product was nine times more effective in preventing S. aureus infections than the 1% oil-based product. Differences in the incidence of S. agalactiae infections were nonsignificant within the individual trials. Though ineffective in preventing udder infections oil-based products were effective in creating a supple condition of teat skin.", "contents": "Hygiene in the prevention of udder infections. II. Evaluation of oil-based teat dips. Oil-based teat dips containing iodine at .5 and 1% were compared in a series of studies with two water-based iodophor products of the same concentration and also with undipped controls. When the two types of products were tested at .5% under conditions of natural infection, the water-based dip was four times more effective than the oil-based dip at preventing Staphylococcus aureus infections. In three subsequent trials teats were experimentally exposed to S. aureus and Streptococcus agalactiae. Five times more S. aureus infections developed in a group dipped with the oil-based dip at 1% iodine with an undipped control yielded no significant difference for S. aureus infections. The 1% iodine water-based product was nine times more effective in preventing S. aureus infections than the 1% oil-based product. Differences in the incidence of S. agalactiae infections were nonsignificant within the individual trials. Though ineffective in preventing udder infections oil-based products were effective in creating a supple condition of teat skin."}
{"id": "PMID:1089683", "title": "Hygiene in the prevention of udder infections. III. Effectiveness of 59 teat dips for reducing bacterial populations on teat skin.", "content": "Fifty-nine teat dips were tested for effectiveness in reducing populations of experimentally applied Streptococcus agalactiae and Staphylococcus aureus on teat skin. The results varied widely among products. Populations of S. agalactiae recovered from dipped teats ranged from a 98.3% reduction to a 147.4% increase as compared to undipped controls. Reductions in recoverable S. aureus ranged from 98.5 to 43.6%. The products yielding the highest germicidal activity did not conform to a consistent pattern in type and concentration of active ingredients, presence or lack of specific emollients, or pH. Approximately 20% of the products yielded a low activity. Each of six oil-based products performed poorly and frequently increased numbers of organisms.", "contents": "Hygiene in the prevention of udder infections. III. Effectiveness of 59 teat dips for reducing bacterial populations on teat skin. Fifty-nine teat dips were tested for effectiveness in reducing populations of experimentally applied Streptococcus agalactiae and Staphylococcus aureus on teat skin. The results varied widely among products. Populations of S. agalactiae recovered from dipped teats ranged from a 98.3% reduction to a 147.4% increase as compared to undipped controls. Reductions in recoverable S. aureus ranged from 98.5 to 43.6%. The products yielding the highest germicidal activity did not conform to a consistent pattern in type and concentration of active ingredients, presence or lack of specific emollients, or pH. Approximately 20% of the products yielded a low activity. Each of six oil-based products performed poorly and frequently increased numbers of organisms."}
{"id": "PMID:1089684", "title": "Estrus detection and estrus detection aids.", "content": "There is no substitute for good management. Each animal should be identified carefully. The herds man should know all signs of estrus or impending estrus. In addition, for visual detection of estrus to be highly effective, sufficient time must be taken at least twice a day to catch animals with a short estrus period. Presently there are two visual aids to estrus detection which provide potential 24-h surveillance. One aid is a pressure sensitive device mounted on the back of each cow which can be triggered when the cow stands for mounting. The second one is a marking device worn by sexually aggressive animals which will stripe with colored ink the back of estrous animals as the marker animal mounts and dismounts. Both devices are effective when used properly. Other tests of changes in cervical mucus, vaginal characteristics, temperature, blood flow, and hormone changes in blood and milk are either not suffciently reliable or else simple enough yet to be practical aids for routine detection of estrus in dairy cattle. Milk progesterone can assist in characterizing problem cows.", "contents": "Estrus detection and estrus detection aids. There is no substitute for good management. Each animal should be identified carefully. The herds man should know all signs of estrus or impending estrus. In addition, for visual detection of estrus to be highly effective, sufficient time must be taken at least twice a day to catch animals with a short estrus period. Presently there are two visual aids to estrus detection which provide potential 24-h surveillance. One aid is a pressure sensitive device mounted on the back of each cow which can be triggered when the cow stands for mounting. The second one is a marking device worn by sexually aggressive animals which will stripe with colored ink the back of estrous animals as the marker animal mounts and dismounts. Both devices are effective when used properly. Other tests of changes in cervical mucus, vaginal characteristics, temperature, blood flow, and hormone changes in blood and milk are either not suffciently reliable or else simple enough yet to be practical aids for routine detection of estrus in dairy cattle. Milk progesterone can assist in characterizing problem cows."}
{"id": "PMID:1089694", "title": "Rehabilitation of the atrophic edentulous maxilla by bone grafting.", "content": "Transoral bone grafting is a useful method for the reconstruction of the atrophic maxillary residual ridge in patients who cannot retain complete dentures. The indications, criteria for patient selection, and the technique used have been presented.", "contents": "Rehabilitation of the atrophic edentulous maxilla by bone grafting. Transoral bone grafting is a useful method for the reconstruction of the atrophic maxillary residual ridge in patients who cannot retain complete dentures. The indications, criteria for patient selection, and the technique used have been presented."}
{"id": "PMID:1089695", "title": "Further studies on gold alloys used in fabrication of porcelain-fused-to-metal restorations.", "content": "Composition, microstructure, castability, mechanical properties, and heat treatment characteristics of two gold-palladium-silver-based alloys were studied. The materials exhibited compositional as well as microstructural differences. Clinically acceptable castings could not be obtained when manufacturers' recommended casting temperatures were used. Ultimate tensile strength, yield strength, modulus of elasticity, and Brinell hardness values for the alloys were comparable. The elastic limit of Cameo, however, was significantly higher than that of vivo-star. Maximum rehardening of annealed castings occurred on reheat treatment at temperatures between 1,200 and 1,300 F. As-cast specimens, however, were not heat hardenable. The sequence of heat treatments used in the application of porcelain reduced slightly the hardness of both alloys. Hardness of the metal substructures was not increased by return of porcelain-coated specimens to a 1,250 F oven for final heat treatment.", "contents": "Further studies on gold alloys used in fabrication of porcelain-fused-to-metal restorations. Composition, microstructure, castability, mechanical properties, and heat treatment characteristics of two gold-palladium-silver-based alloys were studied. The materials exhibited compositional as well as microstructural differences. Clinically acceptable castings could not be obtained when manufacturers' recommended casting temperatures were used. Ultimate tensile strength, yield strength, modulus of elasticity, and Brinell hardness values for the alloys were comparable. The elastic limit of Cameo, however, was significantly higher than that of vivo-star. Maximum rehardening of annealed castings occurred on reheat treatment at temperatures between 1,200 and 1,300 F. As-cast specimens, however, were not heat hardenable. The sequence of heat treatments used in the application of porcelain reduced slightly the hardness of both alloys. Hardness of the metal substructures was not increased by return of porcelain-coated specimens to a 1,250 F oven for final heat treatment."}
{"id": "PMID:1089697", "title": "Humoral and cellular immunity in asthma.", "content": "Parameters of humoral and cellular immunity have been measured in 91 asthmatic patients. Mean serum levels of IgG and IgE were raised. IgG levels were higher in those with a family history of asthma. IgE levels were higher in those with a past history of atopic eczema, but intrinsic and extrinsic asthma could not be differentiated on the basis of IgE levels. Thirteen of 74 patients failed to respond to tetanus immunization, while only 1 failed to respond to Salmonella typhi H antigen. Tetanus nonresponders had a raised mean serum IgA level, reduced spontaneous lymphocyte tritiated thymidine uptake, and reduced thymidine uptake in fetal calf serum. Eight of 87 patients failed to mount delayed hypersensitivity reactions to a battery of five intradermal antigens. The tritiated thymidine uptake of lymphocytes stimulated with phytohemagglutinin was normal in autologous serum, but reduced in fetal calf serum. The data support the hypothesis that asthma may be associated with immunodeficiency states.", "contents": "Humoral and cellular immunity in asthma. Parameters of humoral and cellular immunity have been measured in 91 asthmatic patients. Mean serum levels of IgG and IgE were raised. IgG levels were higher in those with a family history of asthma. IgE levels were higher in those with a past history of atopic eczema, but intrinsic and extrinsic asthma could not be differentiated on the basis of IgE levels. Thirteen of 74 patients failed to respond to tetanus immunization, while only 1 failed to respond to Salmonella typhi H antigen. Tetanus nonresponders had a raised mean serum IgA level, reduced spontaneous lymphocyte tritiated thymidine uptake, and reduced thymidine uptake in fetal calf serum. Eight of 87 patients failed to mount delayed hypersensitivity reactions to a battery of five intradermal antigens. The tritiated thymidine uptake of lymphocytes stimulated with phytohemagglutinin was normal in autologous serum, but reduced in fetal calf serum. The data support the hypothesis that asthma may be associated with immunodeficiency states."}
{"id": "PMID:1089698", "title": "The presence of IgE on the surface of lymphocytes in nasal polyps.", "content": "The presence of IgE-bearing lymphocytes in nasal polyps was correlated with the patients' atopic status. Following surgical removal, the polyp tissue was treated with hyaluronidase and a single-cell suspension was obtained. Lymphocytes were isolated by gradient centrifugation, and the fluorescent antibody technique was used to study the presence of various immunoglobulin markers on the surface of lymphocytes. The presence of IgE-bearing cells was correlated with serum IgE levels, history of allergy, and skin test reactions. Patients with a positive atopic history had intermediate to high serum IgE levels. There was no correlation between IgE level and skin reactivity in these patients. Good correlation was obtained between the number of IgE-bearing cells in nasal polyps and serum IgE levels in atopic patients. The IgE-bearing cells represented 10 to 40 per cent of total immunoglobulin-bearing lymphocytes. No IgE-bearing cells were detected in five of six patients with a negative atopic history and negative skin tests. Thus IgE may be synthesized within nasal polyps of atopic patients, and the polyps in atopic patients may have a different etiology from those in nonatopic patients.", "contents": "The presence of IgE on the surface of lymphocytes in nasal polyps. The presence of IgE-bearing lymphocytes in nasal polyps was correlated with the patients' atopic status. Following surgical removal, the polyp tissue was treated with hyaluronidase and a single-cell suspension was obtained. Lymphocytes were isolated by gradient centrifugation, and the fluorescent antibody technique was used to study the presence of various immunoglobulin markers on the surface of lymphocytes. The presence of IgE-bearing cells was correlated with serum IgE levels, history of allergy, and skin test reactions. Patients with a positive atopic history had intermediate to high serum IgE levels. There was no correlation between IgE level and skin reactivity in these patients. Good correlation was obtained between the number of IgE-bearing cells in nasal polyps and serum IgE levels in atopic patients. The IgE-bearing cells represented 10 to 40 per cent of total immunoglobulin-bearing lymphocytes. No IgE-bearing cells were detected in five of six patients with a negative atopic history and negative skin tests. Thus IgE may be synthesized within nasal polyps of atopic patients, and the polyps in atopic patients may have a different etiology from those in nonatopic patients."}
{"id": "PMID:1089706", "title": "Clostridium botulinum in Scottish fish farms and farmed trout.", "content": "Rainbow trout and specimens of pond mud were collected from three fish farms and examined for the presence of Clostridium botulinum. Two of the farms were constructed with concrete channels and one was mud-bottomed. Cl. botulinum was isolated only from the mud-bottomed farm (24% of muds), and the isolates were all non-proteolytic type B. The implications of the presence of Cl. botulinum spores in the mud of fish farms is discussed.", "contents": "Clostridium botulinum in Scottish fish farms and farmed trout. Rainbow trout and specimens of pond mud were collected from three fish farms and examined for the presence of Clostridium botulinum. Two of the farms were constructed with concrete channels and one was mud-bottomed. Cl. botulinum was isolated only from the mud-bottomed farm (24% of muds), and the isolates were all non-proteolytic type B. The implications of the presence of Cl. botulinum spores in the mud of fish farms is discussed."}
{"id": "PMID:1089707", "title": "An \"in-production\" method for testing the sterility of infusion fluids.", "content": "The addition of dehydrated broth powder to a random selection of bottles from each batch of infusion fluids before sterilization, followed by incubation of the bottles after sterilization, provides a method of sterilty testing which possesses many advantages over the traditional method of culturing small samples from bottles after sterilization.", "contents": "An \"in-production\" method for testing the sterility of infusion fluids. The addition of dehydrated broth powder to a random selection of bottles from each batch of infusion fluids before sterilization, followed by incubation of the bottles after sterilization, provides a method of sterilty testing which possesses many advantages over the traditional method of culturing small samples from bottles after sterilization."}
{"id": "PMID:1089708", "title": "Identification of two populations of immunoglobulin-bearing lymphocytes in man.", "content": "Human peripheral blood lymphocytes bearing easily detectable surface immunoglobulin were quantitated with routine immunofluorescence procedures. The mean value in 17 healthy adults were 18% (range 9 to 30%). When the cells were preincubated at 37 degrees C and washed at the same temperature before staining, the mean value decreased to 9% (range 4.5 to 18%) (p less than 0.001). The mean value increased to 32% (range 15 to 49%) when the cells were prepared and washed at 4 degrees C, and this value was also significantly different from values at room temperature (p less than 0.01). These temperature-related effects were explained by IgG that remained bound to the cell membrane at 4 degrees C, but eluted at 37 degrees C in serum-free medium. The total number of Ig-bearing lymphocytes and T lymphocytes determined by rosette formation with sheep erythrocytes approached 100%. These studies indicate two populations of Ig-bearing lymphocytes in healthy subjects: one with surface-stable Ig determinants, and another that lacks these markers, but has receptors capable of binding IgG.", "contents": "Identification of two populations of immunoglobulin-bearing lymphocytes in man. Human peripheral blood lymphocytes bearing easily detectable surface immunoglobulin were quantitated with routine immunofluorescence procedures. The mean value in 17 healthy adults were 18% (range 9 to 30%). When the cells were preincubated at 37 degrees C and washed at the same temperature before staining, the mean value decreased to 9% (range 4.5 to 18%) (p less than 0.001). The mean value increased to 32% (range 15 to 49%) when the cells were prepared and washed at 4 degrees C, and this value was also significantly different from values at room temperature (p less than 0.01). These temperature-related effects were explained by IgG that remained bound to the cell membrane at 4 degrees C, but eluted at 37 degrees C in serum-free medium. The total number of Ig-bearing lymphocytes and T lymphocytes determined by rosette formation with sheep erythrocytes approached 100%. These studies indicate two populations of Ig-bearing lymphocytes in healthy subjects: one with surface-stable Ig determinants, and another that lacks these markers, but has receptors capable of binding IgG."}
{"id": "PMID:1089709", "title": "Subepidermal deposition of immunoglobulin in NZB/NZW F hybrid mice.", "content": "The dermal-epidermal junction of NZB/NZW F1 hybrid mice is a site of immunoglobulin (Ig) deposition. This produces a fluorescent band which is very similar to that seen in the skin of patients with systemic lupus erythematosus. The deposition of Ig in the mouse skin is first observed at six months of age and increases in frequency thereafter. After an initial lag of 1 to 2 months, it parallels the age-related deposition of Ig in the kidney. The observed temporal relationship between glomerular and cutaneous Ig deposition supports the concept that the presence of immunoglobulin in the skin is highly indicative of the presence of immune complexes in the kidney.", "contents": "Subepidermal deposition of immunoglobulin in NZB/NZW F hybrid mice. The dermal-epidermal junction of NZB/NZW F1 hybrid mice is a site of immunoglobulin (Ig) deposition. This produces a fluorescent band which is very similar to that seen in the skin of patients with systemic lupus erythematosus. The deposition of Ig in the mouse skin is first observed at six months of age and increases in frequency thereafter. After an initial lag of 1 to 2 months, it parallels the age-related deposition of Ig in the kidney. The observed temporal relationship between glomerular and cutaneous Ig deposition supports the concept that the presence of immunoglobulin in the skin is highly indicative of the presence of immune complexes in the kidney."}
{"id": "PMID:1089710", "title": "E receptors on blasts from untreated acute lymphocytic leukemia (ALL):  comparison of temperature dependence of E rosettes formed by normal and leukemic lymphoid cells.", "content": "The presence of receptors for sheep erythrocytes (E) and sufrace Ig on the bone marrow blasts was investigated in 29 children with untreated acute lymphocytic leukemia (ALL). In 6 of them more than 50% of the bone marrow blasts formed E rosettes, while in none of the 29 were surface Ig detected. The six children with rosette-forming blasts had WBS greater than 5 times 10-4/mm-3 at admission and 4 of them presented with thymic enlargement. E-positive ALL blasts and normal human thymocytes formed rosettes after incubation for 1 hr at 4 degrees C or 37 degrees C. In contrast, normal human peripheral T lymphocytes from blood, spleen, and pleural fluid also formed rosettes at 4 degrees C but these rosettes dissociated at 37 degrees C. In two patients with E-positive ALL, the disappearance from blood of cells forming rosettes at 37 degrees C during the 1st week of treatments paralleled the reduction in circulating blasts. Conversely, after 6 days of therapy almost one-half of the remaining cells formed rosettes at 4 degrees C and had the morphologic features of normal lymphocytes. We conclude that, in contrast to peripheral T cells, normal thymic cells and E-positive blasts share the property of forming E rosettes after 1-hr incubation at 37 degrees C. In patients with E-positive ALL this property may be used to evaluate drug effects upon leukemic and normal T lymphocytes.", "contents": "E receptors on blasts from untreated acute lymphocytic leukemia (ALL):  comparison of temperature dependence of E rosettes formed by normal and leukemic lymphoid cells. The presence of receptors for sheep erythrocytes (E) and sufrace Ig on the bone marrow blasts was investigated in 29 children with untreated acute lymphocytic leukemia (ALL). In 6 of them more than 50% of the bone marrow blasts formed E rosettes, while in none of the 29 were surface Ig detected. The six children with rosette-forming blasts had WBS greater than 5 times 10-4/mm-3 at admission and 4 of them presented with thymic enlargement. E-positive ALL blasts and normal human thymocytes formed rosettes after incubation for 1 hr at 4 degrees C or 37 degrees C. In contrast, normal human peripheral T lymphocytes from blood, spleen, and pleural fluid also formed rosettes at 4 degrees C but these rosettes dissociated at 37 degrees C. In two patients with E-positive ALL, the disappearance from blood of cells forming rosettes at 37 degrees C during the 1st week of treatments paralleled the reduction in circulating blasts. Conversely, after 6 days of therapy almost one-half of the remaining cells formed rosettes at 4 degrees C and had the morphologic features of normal lymphocytes. We conclude that, in contrast to peripheral T cells, normal thymic cells and E-positive blasts share the property of forming E rosettes after 1-hr incubation at 37 degrees C. In patients with E-positive ALL this property may be used to evaluate drug effects upon leukemic and normal T lymphocytes."}
{"id": "PMID:1089711", "title": "Depression of humoral immunity to sheep erythrocytes in vitro by Friend virus leukemic spleen cells: induction of resistance by statolon.", "content": "The in vitro antibody plaque-forming cell (PFC) response of normal spleen cells exposed to sheep erythrocytes in Marbrook chambers was depressed by the addition of Friend virus (FV) leukemic spleen cells. Fewer than 10-5 leukemic cells inhibited the PFC response of 10-7 normal cells. This immunodepression could not be produced with sonicated, irradiated, or mitomycin C-treated leukemic cells or with cellfree FV. It could be blocked by FV immune serum but was unaffected by high titers of purified interferon. Interferon did not inhibit the immune response of normal spleen cells to sheep erythrocytes. Spleen cells from mice treated with statolon or chlorite-oxidized oxyamylose statolon but not Newcastle disease virus or poly rI:poly rC were resistant to immunodepression in vitro by FV leukemic spleen cells. The unique ability of statolon to prevent immunodepression by leukemic spleen cells may be the basis of its FV leukemo-suppressive activity in vivo.", "contents": "Depression of humoral immunity to sheep erythrocytes in vitro by Friend virus leukemic spleen cells: induction of resistance by statolon. The in vitro antibody plaque-forming cell (PFC) response of normal spleen cells exposed to sheep erythrocytes in Marbrook chambers was depressed by the addition of Friend virus (FV) leukemic spleen cells. Fewer than 10-5 leukemic cells inhibited the PFC response of 10-7 normal cells. This immunodepression could not be produced with sonicated, irradiated, or mitomycin C-treated leukemic cells or with cellfree FV. It could be blocked by FV immune serum but was unaffected by high titers of purified interferon. Interferon did not inhibit the immune response of normal spleen cells to sheep erythrocytes. Spleen cells from mice treated with statolon or chlorite-oxidized oxyamylose statolon but not Newcastle disease virus or poly rI:poly rC were resistant to immunodepression in vitro by FV leukemic spleen cells. The unique ability of statolon to prevent immunodepression by leukemic spleen cells may be the basis of its FV leukemo-suppressive activity in vivo."}
{"id": "PMID:1089712", "title": "Immunosuppression by leukemia viruses. Effect of Friend leukemia virus on humoral immune competence of leukemia-resistant C57BL/6 mice.", "content": "Infection of genetically resistant C57BL/6 mice with Friend leukemia virus resulted in a marked but transient immunodepression of the humoral immune response to sheep erythrocytes. The primary immune response to sheep erythrocytes was depressed in mice infected 1 or 3 days before challenge immunization, but no suppression was observed when the interval was greater. The suppression coincided with the time of transient viremia in the mice. The secondary immune response to sheep erythrocytes was inhibited when virus was injected within a few days before booster immunization. Induction of immunologic \"memory\" to sheep erythrocytes was also blocked in C57BL/6 mice given virus before priming with the SRBC. The immunosuppressive activity appeared due to a marked but transient effect of the virus on antibody precursor cells, as ascertained by cell transfer experiments. These observations are pertinent to the general question to the cellular site of action of immunosuppressive tumor viruses and the relationship between immunosuppression and the neoplastic properties of the RNA viruses.", "contents": "Immunosuppression by leukemia viruses. Effect of Friend leukemia virus on humoral immune competence of leukemia-resistant C57BL/6 mice. Infection of genetically resistant C57BL/6 mice with Friend leukemia virus resulted in a marked but transient immunodepression of the humoral immune response to sheep erythrocytes. The primary immune response to sheep erythrocytes was depressed in mice infected 1 or 3 days before challenge immunization, but no suppression was observed when the interval was greater. The suppression coincided with the time of transient viremia in the mice. The secondary immune response to sheep erythrocytes was inhibited when virus was injected within a few days before booster immunization. Induction of immunologic \"memory\" to sheep erythrocytes was also blocked in C57BL/6 mice given virus before priming with the SRBC. The immunosuppressive activity appeared due to a marked but transient effect of the virus on antibody precursor cells, as ascertained by cell transfer experiments. These observations are pertinent to the general question to the cellular site of action of immunosuppressive tumor viruses and the relationship between immunosuppression and the neoplastic properties of the RNA viruses."}
{"id": "PMID:1089713", "title": "Differential IgA repopulation after transfer of autologous and allogeneic rabbit Peyer's patch cells.", "content": "The distribution and differentiation of rabbit Peyer's patch cells were studied in lethally x-irradiated autologou, and allogeneic recipients, 6 days after i.v. transfer. Immunoglobulin Ig-containing cells were detected in both frozen tissue sections and fixed smears of single cell suspensions of spleens using monospecific anti-alpha, -mu, and -iota antisera in direct immunofluorescence. The tissues of lethally x-irradiated rabbits which had not received any cells were almost devoid of Ig-containing cells whereas the intestinal lamina propria of both autologous and allogeneic Peyer's patch cell recipients had approximately equal numbers of predominantly IgA-containing cells. In the spleen of allogeneic recipients, many large colonies of IgA-containing cells were seen and these cells made up 4.6 to 16.9% (mean 8%) of all spleen cell suspension. The spleens of autologous recipients contained fewer and smaller colonies of IgA cells which made up only 0.5% of all cells in spleen cell suspensions. The numbers of IgM- and IgG-containing spleen cells were small in all animals, however, recipients of allogeneic Peyer's patch cells had four to five times as many as either non-reconstituted lethally irradiated rabbits or autologous recipients. These data confirm that rabbit Peyer's patches are relatively rich in precursors of IgA-producing cells and suggest that histocompatibility differences may either potentiate differentiation of IgA production or lead to trapping of allogeneic Peyer's patch cells in the spleen. Although these experiments did not elucidate the mechanisms of differentiation of IgA-producing cells, the fact that no differences were seen between numbers of IgA cells in gut lamina propria after either autologous or allogeneic cell transfer suggest that different mechanisms may be responsible for appearance of these cells in gut and spleen.", "contents": "Differential IgA repopulation after transfer of autologous and allogeneic rabbit Peyer's patch cells. The distribution and differentiation of rabbit Peyer's patch cells were studied in lethally x-irradiated autologou, and allogeneic recipients, 6 days after i.v. transfer. Immunoglobulin Ig-containing cells were detected in both frozen tissue sections and fixed smears of single cell suspensions of spleens using monospecific anti-alpha, -mu, and -iota antisera in direct immunofluorescence. The tissues of lethally x-irradiated rabbits which had not received any cells were almost devoid of Ig-containing cells whereas the intestinal lamina propria of both autologous and allogeneic Peyer's patch cell recipients had approximately equal numbers of predominantly IgA-containing cells. In the spleen of allogeneic recipients, many large colonies of IgA-containing cells were seen and these cells made up 4.6 to 16.9% (mean 8%) of all spleen cell suspension. The spleens of autologous recipients contained fewer and smaller colonies of IgA cells which made up only 0.5% of all cells in spleen cell suspensions. The numbers of IgM- and IgG-containing spleen cells were small in all animals, however, recipients of allogeneic Peyer's patch cells had four to five times as many as either non-reconstituted lethally irradiated rabbits or autologous recipients. These data confirm that rabbit Peyer's patches are relatively rich in precursors of IgA-producing cells and suggest that histocompatibility differences may either potentiate differentiation of IgA production or lead to trapping of allogeneic Peyer's patch cells in the spleen. Although these experiments did not elucidate the mechanisms of differentiation of IgA-producing cells, the fact that no differences were seen between numbers of IgA cells in gut lamina propria after either autologous or allogeneic cell transfer suggest that different mechanisms may be responsible for appearance of these cells in gut and spleen."}
{"id": "PMID:1089714", "title": "Studies on the composition of adjuvants which selectively enhance delayed-type hypersensitivity to lipid conjugated protein antigens.", "content": "Hen egg albumin (HEA), heavily conjugated with dodecanoic acid (D-HEA), stimulated sustained delayed type hypersensitivity (DTH) specific for HEA without detectable antibody formation in guinea pigs. An oil-in-water emulsion containing purified BCG cell walls attached to the oil drops was found to be a very effective adjuvant for enhancing DTH to D-HEA, but not to HEA. Animals immunized with D-HEA in the BCG cell wall emulsion produced skin test reactions 2.4 cm in diameter when challenged with HEA 21 days after a single immunization. Control animals immunized with D-HEA in saline produced skin reactions 1 cm in diameter to similar challenge. Neither group of animals produced detectable antibody to HEA-OR D-HEAmThe emulsion had no adjuvant effect if the BCG cell walls were suspended in the aqueous phase and not attached to the oil droplets. Dodecanoic acid conjugated Salmonella typhi organisms could be used in place of the BCG cell walls to produce effective adjuvant preparations. Fruend's complete adjuvant and other water-in-oil emulsions, however, were found to be ineffective adjuvants for enhancing the degree of DTH produced by D-HEA. Experiments with autoradiography demonstrated that effective adjuvant preparations promote the localization and retention of both 125-I-labeled D-HEA and 125-I-labeled BCG cell walls in the paracortical area of lymph nodes where they are in close proximity to many T-type lymphocytes which proliferate in the induction of DTH.", "contents": "Studies on the composition of adjuvants which selectively enhance delayed-type hypersensitivity to lipid conjugated protein antigens. Hen egg albumin (HEA), heavily conjugated with dodecanoic acid (D-HEA), stimulated sustained delayed type hypersensitivity (DTH) specific for HEA without detectable antibody formation in guinea pigs. An oil-in-water emulsion containing purified BCG cell walls attached to the oil drops was found to be a very effective adjuvant for enhancing DTH to D-HEA, but not to HEA. Animals immunized with D-HEA in the BCG cell wall emulsion produced skin test reactions 2.4 cm in diameter when challenged with HEA 21 days after a single immunization. Control animals immunized with D-HEA in saline produced skin reactions 1 cm in diameter to similar challenge. Neither group of animals produced detectable antibody to HEA-OR D-HEAmThe emulsion had no adjuvant effect if the BCG cell walls were suspended in the aqueous phase and not attached to the oil droplets. Dodecanoic acid conjugated Salmonella typhi organisms could be used in place of the BCG cell walls to produce effective adjuvant preparations. Fruend's complete adjuvant and other water-in-oil emulsions, however, were found to be ineffective adjuvants for enhancing the degree of DTH produced by D-HEA. Experiments with autoradiography demonstrated that effective adjuvant preparations promote the localization and retention of both 125-I-labeled D-HEA and 125-I-labeled BCG cell walls in the paracortical area of lymph nodes where they are in close proximity to many T-type lymphocytes which proliferate in the induction of DTH."}
{"id": "PMID:1089715", "title": "Suppression and enhancement of the T cell-dependent production of antibody to SRBC in vitro by bacterial lipopolysaccharide.", "content": "LPS induced the production of antibody to sheep red blood cells (SRBC) in cultures of spleen cells from normal and T cell-depleted mice, and addition of SRBC to the cultures enchanced this T cell-independent response very little. By contrast, the T cell-dependent production of antibody to SRBC in vitro was suppressed when lipopolysaccharide (LPS) was added at the time when the spleen cells were cultured. Later addition of LPS to spleen cell cultures caused enhancement of antibody production, but only when LPS had not been added before. Addition of T cells that had been primed with SRBC in vivo did not reverse the LPS-induced suppression of antibody production. The data are interpreted to mean that either B cells are rendered incapable of receiving T cell signals in the presence of LPS or that LPS interferes with the appropriate association of cellular components which cooperate in the immune response to SRBC.", "contents": "Suppression and enhancement of the T cell-dependent production of antibody to SRBC in vitro by bacterial lipopolysaccharide. LPS induced the production of antibody to sheep red blood cells (SRBC) in cultures of spleen cells from normal and T cell-depleted mice, and addition of SRBC to the cultures enchanced this T cell-independent response very little. By contrast, the T cell-dependent production of antibody to SRBC in vitro was suppressed when lipopolysaccharide (LPS) was added at the time when the spleen cells were cultured. Later addition of LPS to spleen cell cultures caused enhancement of antibody production, but only when LPS had not been added before. Addition of T cells that had been primed with SRBC in vivo did not reverse the LPS-induced suppression of antibody production. The data are interpreted to mean that either B cells are rendered incapable of receiving T cell signals in the presence of LPS or that LPS interferes with the appropriate association of cellular components which cooperate in the immune response to SRBC."}
{"id": "PMID:1089716", "title": "Synergistic effects on DNA synthesis of phytohemagglutinin or concanavalin A and lipopolysaccharide in human peripheral blood lymphocytes.", "content": "Mixtures of phytohemagglutinin (PHA) or Concanavalin A (Con A) and lipopolysaccharide (LPS) with human peripheral blood lymphocytes (PBL) resulted in a synergistic effect on DNA synthesis as measured by increased 3H-thymidine uptake. These data were compared to the mitogenic response of human PBL to PHA, Con A, or LPS alone. Various combinations of PHA and Con A usually suppressed DNA synthesis in human PBL. Titration experiments revealed that increasing amounts of LPS, in the presence of a constant amount of Con A, resulted in corresponding increases in the synergistic response of human PBL. Conversely, increasing amounts of Con A, in the presence of a constant amount of LPA did not lead to increases in the amount of synergism. The results are discussed in terms of the participation of additional cells in the synergistic response of human PBL.", "contents": "Synergistic effects on DNA synthesis of phytohemagglutinin or concanavalin A and lipopolysaccharide in human peripheral blood lymphocytes. Mixtures of phytohemagglutinin (PHA) or Concanavalin A (Con A) and lipopolysaccharide (LPS) with human peripheral blood lymphocytes (PBL) resulted in a synergistic effect on DNA synthesis as measured by increased 3H-thymidine uptake. These data were compared to the mitogenic response of human PBL to PHA, Con A, or LPS alone. Various combinations of PHA and Con A usually suppressed DNA synthesis in human PBL. Titration experiments revealed that increasing amounts of LPS, in the presence of a constant amount of Con A, resulted in corresponding increases in the synergistic response of human PBL. Conversely, increasing amounts of Con A, in the presence of a constant amount of LPA did not lead to increases in the amount of synergism. The results are discussed in terms of the participation of additional cells in the synergistic response of human PBL."}
{"id": "PMID:1089717", "title": "Functional heterogeneity among the T-derived lymphocytes of the mouse. II. Sensitivity of subpopulations to anti-thymocyte serum.", "content": "A number of T cell functions were examined in mice after treatment with rabbit anti-mouse thymocyte serum (ATS). Two populations of T cells were distinguished; the first, sensitive, and a second, resistant, to elimination by ATS. The helper cell involved in the primary humoral response to sheep red blood cells (SRBC) was found within the sensitive population, whereas the T-cells required for the priming of mice with SRBC for helper activity or delayed hypersensitivity were found within both populations. Previous work and experiments utilizing both adult thymectomy and ATS treatment indicated that the ATS-sensitive population is long-lived, whereas the ATS-resistant population is short-lived.", "contents": "Functional heterogeneity among the T-derived lymphocytes of the mouse. II. Sensitivity of subpopulations to anti-thymocyte serum. A number of T cell functions were examined in mice after treatment with rabbit anti-mouse thymocyte serum (ATS). Two populations of T cells were distinguished; the first, sensitive, and a second, resistant, to elimination by ATS. The helper cell involved in the primary humoral response to sheep red blood cells (SRBC) was found within the sensitive population, whereas the T-cells required for the priming of mice with SRBC for helper activity or delayed hypersensitivity were found within both populations. Previous work and experiments utilizing both adult thymectomy and ATS treatment indicated that the ATS-sensitive population is long-lived, whereas the ATS-resistant population is short-lived."}
{"id": "PMID:1089718", "title": "Effect of polycations and polyanions on behavior of sheep red blood cell rosettes of human lymphocytes.", "content": "The role of cell surface charges in the behavior of sheep red blood  cells (SRBC) rosettes with human lymphocytes was investigated by using various polycations and polyanions. Polycations (DEAE-dextran, polybrene, poly-L-lysine, spermadine) at appropriate concentrations (1) invariably increased the proportions of early rosettes. This was so irrespective of whether it was the SRBC or the lymphocytes that were being treated with the reagents. (2) The rosettes so formed also consisted of a greater number of red blood cells per rosette (3). On incubating the rosettes at 37 degrees C, they dissociated by capping. The rates of dissociation and capping were retarded by polycation treatment. (4) The resistance to rosette disruption was increased. These properties were very similar to those observed on cells treated with neuraminidase. Treatment of cells with the polyanions heparin and dextran sulfate induced the opposite effects: reduced the number of rosettes and increased the rate of dissociation, and decreased the resistance of rosettes to disruption. It was concluded that alterations of cell surface charges could drastically influence SRBC-lymphocyte interactions. To investigate whether rosette inhibitors could do so by altering cell surface charges, cell were treated with anti-lymphocytic sera, 2,4-dinitrophenol and sodium azide. All three agents inhibited rosette formation. Whereas DEAE-dextran could partially reverse the inhibition by the first two agents, it failed to do so with the third. This suggested that the first two may have inhibited rosette formation by altering the surface charges.", "contents": "Effect of polycations and polyanions on behavior of sheep red blood cell rosettes of human lymphocytes. The role of cell surface charges in the behavior of sheep red blood  cells (SRBC) rosettes with human lymphocytes was investigated by using various polycations and polyanions. Polycations (DEAE-dextran, polybrene, poly-L-lysine, spermadine) at appropriate concentrations (1) invariably increased the proportions of early rosettes. This was so irrespective of whether it was the SRBC or the lymphocytes that were being treated with the reagents. (2) The rosettes so formed also consisted of a greater number of red blood cells per rosette (3). On incubating the rosettes at 37 degrees C, they dissociated by capping. The rates of dissociation and capping were retarded by polycation treatment. (4) The resistance to rosette disruption was increased. These properties were very similar to those observed on cells treated with neuraminidase. Treatment of cells with the polyanions heparin and dextran sulfate induced the opposite effects: reduced the number of rosettes and increased the rate of dissociation, and decreased the resistance of rosettes to disruption. It was concluded that alterations of cell surface charges could drastically influence SRBC-lymphocyte interactions. To investigate whether rosette inhibitors could do so by altering cell surface charges, cell were treated with anti-lymphocytic sera, 2,4-dinitrophenol and sodium azide. All three agents inhibited rosette formation. Whereas DEAE-dextran could partially reverse the inhibition by the first two agents, it failed to do so with the third. This suggested that the first two may have inhibited rosette formation by altering the surface charges."}
{"id": "PMID:1089719", "title": "The effect of plasmacytomas on serum immunoglobulin levels of BALB/c mice.", "content": "The effect of tumor growth on serum immunoglobulin levels and on the immune response to sheep erythrocytes (SRBC) was studied in BALB/c mice bearing MOPC-315 (IgA), MOPC-460 (IgA), MOPC-173 (IgG2a) and MOPC-104E (IgM) to gain insight into the immunologic competence of the plasmacytoma-bearing host. The initial increase of myeloma protein coincided with the first appearance of the tumor and increased as the tumor progressed. However, at the time of death there was little correlation between spleen weights, tumor size, and myeloma-protein levels. The mean serum concentration of the myeloma proteins reached a higher level in the mice bearing tumors transplanted i.p. compared to those with tumors transferred subcutaneously (s.c.). Non-myeloma immunoglobulin levels in the serum were reduced: IgM was significantly lowered in the presence of MOPC-315 injected i.p. and MOPC-460 injected s.c. and the IgG2 levels were depressed in mice injected i.p. with MOPC-315 and MOPC-104E. The only significant reduction of IgA levels was seen when MOPC-173 was transplanted i.p. The decreases observed in immunoglobulin levels correlated with plasmacytoma growth. They were specific for myeloma and were not due to tumor growth per se since the levels of all immunoglobulins tested increased in the presence of Sarcoma 37, a pleomorphic neoplasm. The primary plaque-forming cell (PFC) response of tumor-bearing animals after the injection of 0.5 ml of 10% SRBC was either similar or enhanced compared to the controls. However, with a lower SRBC dosage (0.5 ml of 2% SRBC) the indirect PFC were reduced with mice bearing MOPC-104E and MOPC-173. Tumor sizes did not seem to correlate with reduction of the PFC response. MOPC-460-bearing mice had a comparable number of PFC per spleen to those of the controls, but reduced numbers when calculated per 10 spleen cells. Consistently, hemagglutination titers were reduced in all tumor-bearing animals. The number of direct and indirect PFC per spleen was increased in mice bearing Sarcoma 37, compared to the controls. The possible implications of these findings are discussed.", "contents": "The effect of plasmacytomas on serum immunoglobulin levels of BALB/c mice. The effect of tumor growth on serum immunoglobulin levels and on the immune response to sheep erythrocytes (SRBC) was studied in BALB/c mice bearing MOPC-315 (IgA), MOPC-460 (IgA), MOPC-173 (IgG2a) and MOPC-104E (IgM) to gain insight into the immunologic competence of the plasmacytoma-bearing host. The initial increase of myeloma protein coincided with the first appearance of the tumor and increased as the tumor progressed. However, at the time of death there was little correlation between spleen weights, tumor size, and myeloma-protein levels. The mean serum concentration of the myeloma proteins reached a higher level in the mice bearing tumors transplanted i.p. compared to those with tumors transferred subcutaneously (s.c.). Non-myeloma immunoglobulin levels in the serum were reduced: IgM was significantly lowered in the presence of MOPC-315 injected i.p. and MOPC-460 injected s.c. and the IgG2 levels were depressed in mice injected i.p. with MOPC-315 and MOPC-104E. The only significant reduction of IgA levels was seen when MOPC-173 was transplanted i.p. The decreases observed in immunoglobulin levels correlated with plasmacytoma growth. They were specific for myeloma and were not due to tumor growth per se since the levels of all immunoglobulins tested increased in the presence of Sarcoma 37, a pleomorphic neoplasm. The primary plaque-forming cell (PFC) response of tumor-bearing animals after the injection of 0.5 ml of 10% SRBC was either similar or enhanced compared to the controls. However, with a lower SRBC dosage (0.5 ml of 2% SRBC) the indirect PFC were reduced with mice bearing MOPC-104E and MOPC-173. Tumor sizes did not seem to correlate with reduction of the PFC response. MOPC-460-bearing mice had a comparable number of PFC per spleen to those of the controls, but reduced numbers when calculated per 10 spleen cells. Consistently, hemagglutination titers were reduced in all tumor-bearing animals. The number of direct and indirect PFC per spleen was increased in mice bearing Sarcoma 37, compared to the controls. The possible implications of these findings are discussed."}
{"id": "PMID:1089720", "title": "Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. II. Functional studies.", "content": "The alloantigenic specificity Ly-4.2 can be detected on a proportion of lymphocytes by the antiserum (BALB/c times SWR)F1 anti-B10.D2. In the preceding study it was shown that these lymphocytes were not thymus-derived (T) cells, as they were Thy-1 (theta)(-), and were therefore presumably B (bone marrow-derived) cells. Evidence is now presented for the reaction of the Ly-4.2 antiserum with functional B cells. Thus, the LY-4.2 and Thy-1.2 specificities were detected on antigen-binding rosette-forming cells (RFC) in mice both immune and non-immune to sheep red cells (SRC). RFC formed to endotoxin lipopolysaccharide (LPS) were also Ly-4.2(+). Memory cells to both SRC andLPS could be detected with anti-Ly-4.2 and anti-Thy-1.2 antisera, thereby indicating that both T and B cells are involved in memory to these antigens. Both direct and indirect antibody-forming cells (the PFC) could be inhibited, in vitro, by anti-Ly-4.2 antiserum, although it is likely that not all PFC are Ly-4.2(+). Neither of the specificities Ly-4.2 nor Thy-1.2 were detected on the bone marrow precursor of the splenic colony forming unit (the CFU). In an assay for B cells, the treatment of lymph node or spleen cells with anti-Ly-4.2 before transfer to irradiated recipients could inhibit the ability of these cells to make PFC to SRC, and this capacity could only be restored by bone marrow cells and not by thymus cells. These studies provide clear evidence for the presence of the Ly-4.2 specificity on antibody-forming cells and their precursor (B cells).", "contents": "Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. II. Functional studies. The alloantigenic specificity Ly-4.2 can be detected on a proportion of lymphocytes by the antiserum (BALB/c times SWR)F1 anti-B10.D2. In the preceding study it was shown that these lymphocytes were not thymus-derived (T) cells, as they were Thy-1 (theta)(-), and were therefore presumably B (bone marrow-derived) cells. Evidence is now presented for the reaction of the Ly-4.2 antiserum with functional B cells. Thus, the LY-4.2 and Thy-1.2 specificities were detected on antigen-binding rosette-forming cells (RFC) in mice both immune and non-immune to sheep red cells (SRC). RFC formed to endotoxin lipopolysaccharide (LPS) were also Ly-4.2(+). Memory cells to both SRC andLPS could be detected with anti-Ly-4.2 and anti-Thy-1.2 antisera, thereby indicating that both T and B cells are involved in memory to these antigens. Both direct and indirect antibody-forming cells (the PFC) could be inhibited, in vitro, by anti-Ly-4.2 antiserum, although it is likely that not all PFC are Ly-4.2(+). Neither of the specificities Ly-4.2 nor Thy-1.2 were detected on the bone marrow precursor of the splenic colony forming unit (the CFU). In an assay for B cells, the treatment of lymph node or spleen cells with anti-Ly-4.2 before transfer to irradiated recipients could inhibit the ability of these cells to make PFC to SRC, and this capacity could only be restored by bone marrow cells and not by thymus cells. These studies provide clear evidence for the presence of the Ly-4.2 specificity on antibody-forming cells and their precursor (B cells)."}
{"id": "PMID:1089721", "title": "Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity.", "content": "A transplantable, murine reticulum cell sarcoma is described which exhibits the cytologic, adherence, and phagocytic properties of macrophages. It forms specific rosettes with erythrocytes in the presence of the corresponding anti-serum. The ascites cells mediate antibody-dependent cellular immunity as assayed by release of radioactivity from 51Cr-labeled erythrocytes. The contribution of contaminating host cells in the cytotoxic reaction was ruled out by growing the tumor in F1 mice and removing the host cells by anti-H2 serum and complement. The tumor cells have receptors for IgG2a and IgG2b immunoglobulins. The availability of a pure population of effector cells in the immune system allows study of the biochemical processes pursuant to lysis of foreign cells.", "contents": "Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity. A transplantable, murine reticulum cell sarcoma is described which exhibits the cytologic, adherence, and phagocytic properties of macrophages. It forms specific rosettes with erythrocytes in the presence of the corresponding anti-serum. The ascites cells mediate antibody-dependent cellular immunity as assayed by release of radioactivity from 51Cr-labeled erythrocytes. The contribution of contaminating host cells in the cytotoxic reaction was ruled out by growing the tumor in F1 mice and removing the host cells by anti-H2 serum and complement. The tumor cells have receptors for IgG2a and IgG2b immunoglobulins. The availability of a pure population of effector cells in the immune system allows study of the biochemical processes pursuant to lysis of foreign cells."}
{"id": "PMID:1089722", "title": "The plaque cytogram assay. II. Correlation between morphology and density of linear bovine serum albumin buoyant density gradient-separated immunocompetent cells.", "content": "The plaque cytogram assay has been combined with pH 6.8, linear bovine serum albumin (BSA) gradients to demonstrate the correlation between the morphology and buoyant density of immunocompetent cells. Ten types of plaque-forming cells (PFC) and four types of plaques have been identified and enriched in the density gradients. Reproducible density profiles of mouse erythrocytes, lymphoid cells, and PFC have been obtained with BSA gradients. The density peak for mouse erythrocytes was about 1.080 g/ml, while the lymphoid cell density profile had several peaks with a maximum concentration at 1.064 g/ml. Three distinct peaks or shoulders were found in the density profile of hemolytic PFC. Most of the density distributions of the individual morphologic classes of PFC were considerably narrower than that of the entire PFC distribution and were discrete from each other. The combined result that the PFC could be subdivided by morphology and density, and that the two phenomena were correlated establishes that the PFC are truly a heterogeneous population. Whether this heterogeneity is due to different stages in development or due to the existence of differing classes of cells will have to await future experimentation.", "contents": "The plaque cytogram assay. II. Correlation between morphology and density of linear bovine serum albumin buoyant density gradient-separated immunocompetent cells. The plaque cytogram assay has been combined with pH 6.8, linear bovine serum albumin (BSA) gradients to demonstrate the correlation between the morphology and buoyant density of immunocompetent cells. Ten types of plaque-forming cells (PFC) and four types of plaques have been identified and enriched in the density gradients. Reproducible density profiles of mouse erythrocytes, lymphoid cells, and PFC have been obtained with BSA gradients. The density peak for mouse erythrocytes was about 1.080 g/ml, while the lymphoid cell density profile had several peaks with a maximum concentration at 1.064 g/ml. Three distinct peaks or shoulders were found in the density profile of hemolytic PFC. Most of the density distributions of the individual morphologic classes of PFC were considerably narrower than that of the entire PFC distribution and were discrete from each other. The combined result that the PFC could be subdivided by morphology and density, and that the two phenomena were correlated establishes that the PFC are truly a heterogeneous population. Whether this heterogeneity is due to different stages in development or due to the existence of differing classes of cells will have to await future experimentation."}
{"id": "PMID:1089723", "title": "Demonstration of in vitro lymphocyte-mediated cytotoxicity against measles virus in SSPE.", "content": "Patients with subacute sclerosing panencephalitis (SSPE) are presumed to lack specific cellular immunity against measles virus. In order to test this hypothesis in vitro, the interaction between peripheral lymphocytes and measles virus-infected tissue culture cells was investigated in 10 SSPE patients. Human fibroblasts, either uninfected or carrying a persistent measles virus infection, were labeled with 51Cr and incubated with lymphocytes for 18 to 20 hr in the absence of antibody and complement. Peripheral lymphocytes from measles sero-positive and sero-negative individuals were tested, and the system was found to be virus specific. The lymphocytes from the 10 SSPE patients caused specific cytotoxicity of target cells. A correlation was not found between antibody titer and specific 51Cr release. It could also be domonstrated that target cell destruction was not mediated by monocytes or B lymphocytes. These in vitro studies suggest that SSPE patients do not have a specific defect of cellular immunity against measles virus.", "contents": "Demonstration of in vitro lymphocyte-mediated cytotoxicity against measles virus in SSPE. Patients with subacute sclerosing panencephalitis (SSPE) are presumed to lack specific cellular immunity against measles virus. In order to test this hypothesis in vitro, the interaction between peripheral lymphocytes and measles virus-infected tissue culture cells was investigated in 10 SSPE patients. Human fibroblasts, either uninfected or carrying a persistent measles virus infection, were labeled with 51Cr and incubated with lymphocytes for 18 to 20 hr in the absence of antibody and complement. Peripheral lymphocytes from measles sero-positive and sero-negative individuals were tested, and the system was found to be virus specific. The lymphocytes from the 10 SSPE patients caused specific cytotoxicity of target cells. A correlation was not found between antibody titer and specific 51Cr release. It could also be domonstrated that target cell destruction was not mediated by monocytes or B lymphocytes. These in vitro studies suggest that SSPE patients do not have a specific defect of cellular immunity against measles virus."}
{"id": "PMID:1089724", "title": "Characterization of measles virus-specific cytotoxic antibodies by use of a chronically infected cell line.", "content": "The complement-dependent cytotoxic effects of measles virus specific antibodies on a chronically infected cell line were studied by a microcytotoxicity assay. Cell damage was determined by trypan blue staining. Monospecific antisera against the hemagglutinin (HA) and the hemolysin (HL) of the virus were both cytotoxic. Rabbits, immunized with measles virus, developed cytotoxic antibodies in parallel with the appearance of antibodies against the measles virus HA and HL. Human sera with high antibody titers against HA and HL also gave a specific cytotoxic reaction. The main part of the activity was carried by IgG in rabbit sera tested within 2 weeks after primary immunization and only IgM. The serum reaction was characterized by a prozone. The maximal degree of cytotoxicity varied between tests performed on different occasions. Complement, at higher concentrations than were used for analysis of antibody-mediated cytolysis, caused a significant lysis of infected cells. Cerebrospinal fluid (CSF) samples containing measles virus-specific antibodies were investigated. CSF from patients with subacute sclerosing panencephalitis displayed a pronounced cytotoxicity without a prozone. CSF samples from four patients with multiple sclerosis were identified by a specific cytotoxic reaction despite low titers of antibodies against HA and HL.", "contents": "Characterization of measles virus-specific cytotoxic antibodies by use of a chronically infected cell line. The complement-dependent cytotoxic effects of measles virus specific antibodies on a chronically infected cell line were studied by a microcytotoxicity assay. Cell damage was determined by trypan blue staining. Monospecific antisera against the hemagglutinin (HA) and the hemolysin (HL) of the virus were both cytotoxic. Rabbits, immunized with measles virus, developed cytotoxic antibodies in parallel with the appearance of antibodies against the measles virus HA and HL. Human sera with high antibody titers against HA and HL also gave a specific cytotoxic reaction. The main part of the activity was carried by IgG in rabbit sera tested within 2 weeks after primary immunization and only IgM. The serum reaction was characterized by a prozone. The maximal degree of cytotoxicity varied between tests performed on different occasions. Complement, at higher concentrations than were used for analysis of antibody-mediated cytolysis, caused a significant lysis of infected cells. Cerebrospinal fluid (CSF) samples containing measles virus-specific antibodies were investigated. CSF from patients with subacute sclerosing panencephalitis displayed a pronounced cytotoxicity without a prozone. CSF samples from four patients with multiple sclerosis were identified by a specific cytotoxic reaction despite low titers of antibodies against HA and HL."}
{"id": "PMID:1089725", "title": "Studies on the mechanism of stimulation of the humoral response of murine spleen cultures by culture fluids from human monocytes.", "content": "It has been demonstrated that media derived from cultures of human monocytes can stimulate the IgM and IgG anti-sheep erythrocyte response of normal or T cell-deficient murine splenocytes in vitro. This culture fluid also causes a slight stimulation in the rate of B cell division and in the number of antibody-secreting cells occurring in the absence of added antigen. The active factors, which are released from the monocytes during the first 2 days of culture, appear to affect the murine B cells throughout the culture period.", "contents": "Studies on the mechanism of stimulation of the humoral response of murine spleen cultures by culture fluids from human monocytes. It has been demonstrated that media derived from cultures of human monocytes can stimulate the IgM and IgG anti-sheep erythrocyte response of normal or T cell-deficient murine splenocytes in vitro. This culture fluid also causes a slight stimulation in the rate of B cell division and in the number of antibody-secreting cells occurring in the absence of added antigen. The active factors, which are released from the monocytes during the first 2 days of culture, appear to affect the murine B cells throughout the culture period."}
{"id": "PMID:1089726", "title": "Antigen-specific and nonspecific mediators of T cell/B cell cooperation. I. Evidence for their production by different T cells.", "content": "Evidence is presented for two types of helper T cells in the mouse specific for a protein antigen, keyhole limpet hemocyanin (KLH). The first cell is able to help B cells respond to the hapten, trinitrophenyl (TNP), when coupled to KLH presumably through the participation of KLH-specific T cell factors. The second helper cell when stimulated with KLH is able to help B cells respond to red blood cell antigens, but not TNP-KLH, through the production of a non-antigen-specific factor. Both types of helper cells were shown to be present in the spleens of KLH-primed mice, although they occurred with different frequencies. They were shown to be different T cells by their segregation from each other in vitro at limiting dilution.", "contents": "Antigen-specific and nonspecific mediators of T cell/B cell cooperation. I. Evidence for their production by different T cells. Evidence is presented for two types of helper T cells in the mouse specific for a protein antigen, keyhole limpet hemocyanin (KLH). The first cell is able to help B cells respond to the hapten, trinitrophenyl (TNP), when coupled to KLH presumably through the participation of KLH-specific T cell factors. The second helper cell when stimulated with KLH is able to help B cells respond to red blood cell antigens, but not TNP-KLH, through the production of a non-antigen-specific factor. Both types of helper cells were shown to be present in the spleens of KLH-primed mice, although they occurred with different frequencies. They were shown to be different T cells by their segregation from each other in vitro at limiting dilution."}
{"id": "PMID:1089727", "title": "Acute destruction of rat skin grafts by alloantisera.", "content": "Rat skin transplanted to mice or to allogeneic rats can be acutely and severely damaged by injecting the graft recipients with alloimmune serum of appropriate specificity. This susceptibility of rat skin to alloantisera has been obscured in previous studies by the fact that the extent of serum-mediated damage that occurs is decisively influenced by the interval of time between placement of the grafts and the injection of antiserum. Grafts are resistant to damage during the 1st week after transplantation; they rapidly develop sensitivity during the 2nd week, reaching a peak at 14 to 16 days; and during the next few weeks the sensitivity is lost. Grafts surviving beyond 35 to 40 days become totally resistant to antisera. LBNF1 GRAFTS Are more resistant than Le or BN grafts to BN anti-Le serum or Le anti-BN serum, respectively. However, combinations of the two sera can act synergistically to destroy LBN skin that has been grafted to immunosuppressed mice. This form of serum-mediated tissue damage is thought not to play a significant role in skin graft rejection in the usual circumstances of transplantation since cell mediated immunity is well developed before the acquisition by the grafts of sensitivity to humoral agents. Furthermore, antisera from animals that have rejected a single graft have little or no destructive potential, and hyperimmune animals reject skin very shortly after grafting, often before the grafts have become vascularized and hence before they are sensitive to antiserum.", "contents": "Acute destruction of rat skin grafts by alloantisera. Rat skin transplanted to mice or to allogeneic rats can be acutely and severely damaged by injecting the graft recipients with alloimmune serum of appropriate specificity. This susceptibility of rat skin to alloantisera has been obscured in previous studies by the fact that the extent of serum-mediated damage that occurs is decisively influenced by the interval of time between placement of the grafts and the injection of antiserum. Grafts are resistant to damage during the 1st week after transplantation; they rapidly develop sensitivity during the 2nd week, reaching a peak at 14 to 16 days; and during the next few weeks the sensitivity is lost. Grafts surviving beyond 35 to 40 days become totally resistant to antisera. LBNF1 GRAFTS Are more resistant than Le or BN grafts to BN anti-Le serum or Le anti-BN serum, respectively. However, combinations of the two sera can act synergistically to destroy LBN skin that has been grafted to immunosuppressed mice. This form of serum-mediated tissue damage is thought not to play a significant role in skin graft rejection in the usual circumstances of transplantation since cell mediated immunity is well developed before the acquisition by the grafts of sensitivity to humoral agents. Furthermore, antisera from animals that have rejected a single graft have little or no destructive potential, and hyperimmune animals reject skin very shortly after grafting, often before the grafts have become vascularized and hence before they are sensitive to antiserum."}
{"id": "PMID:1089728", "title": "Receptor sites for antigen-antibody complexes on cells derived from solid tumors: detection by means of antibody sensitized sheep erythrocytes labeled with technetium-99m.", "content": "Surface receptor sites for the Fc portion of antigen-antibody complexes were demonstrated on cells derived from three methylcholanthrene-induced fibrosarcomas, one of strain C3H and two of strain BALB/c origin, two spontaneously occurring malignant melanomas (B16 in strain C57BL/6 and Harding-Passey in strain BALB/c mice), a Moloney sarcoma virus-induced tumor of strain BALB/c origin and the Walker 256 carcinosarcoma of Holtzman rats. Primary cell cultures derived from these tumors adsorbed technetium-99m labeled, antibody-sensitized sheep erythrocytes (99mTc EA) as determined either by visual scoring of adherence or radioisotopic quantitation. Depending upon the tumor tested, from 20% to greater than 95% of the target cells absorbed 99mTc EA. All cells lost their reactivity after 1 or 2 passages in vitro, but this was regained after a single passage in vivo. Indicator erythrocytes coated with F(ab')2 fragments of the sensitizing sheep erythrocytes (SRBC) antiserum did not adhere thereby demonstrating that the hemadsorption required an intact Fc portion of the antibody molecule. Adherence of 99mTc EA was blocked by soluble immune complexes prepared with ovalbumin and rabbit antibody directed against it and Escherichia coli 055:B5 lipopolysaccharide and mouse antibody directed against it. Normal rabbit or mouse serum, immune serum, or antigen alone did not block adherence of 99mTc EA thereby demonstrating that the receptors had greater affinity for immune complexes than for either antigen or antibody alone. The existence of membrane receptors on tumor-derived cells which react with the Fc portion of antigen-antibody complexes may provide an explanation for the mechanism by which immune complexes are capable of blocking cell-mediated tumor cell destruction irrespective of whether the receptors are on the tumor cells themselves or on admixed lymphocytes and macrophages.", "contents": "Receptor sites for antigen-antibody complexes on cells derived from solid tumors: detection by means of antibody sensitized sheep erythrocytes labeled with technetium-99m. Surface receptor sites for the Fc portion of antigen-antibody complexes were demonstrated on cells derived from three methylcholanthrene-induced fibrosarcomas, one of strain C3H and two of strain BALB/c origin, two spontaneously occurring malignant melanomas (B16 in strain C57BL/6 and Harding-Passey in strain BALB/c mice), a Moloney sarcoma virus-induced tumor of strain BALB/c origin and the Walker 256 carcinosarcoma of Holtzman rats. Primary cell cultures derived from these tumors adsorbed technetium-99m labeled, antibody-sensitized sheep erythrocytes (99mTc EA) as determined either by visual scoring of adherence or radioisotopic quantitation. Depending upon the tumor tested, from 20% to greater than 95% of the target cells absorbed 99mTc EA. All cells lost their reactivity after 1 or 2 passages in vitro, but this was regained after a single passage in vivo. Indicator erythrocytes coated with F(ab')2 fragments of the sensitizing sheep erythrocytes (SRBC) antiserum did not adhere thereby demonstrating that the hemadsorption required an intact Fc portion of the antibody molecule. Adherence of 99mTc EA was blocked by soluble immune complexes prepared with ovalbumin and rabbit antibody directed against it and Escherichia coli 055:B5 lipopolysaccharide and mouse antibody directed against it. Normal rabbit or mouse serum, immune serum, or antigen alone did not block adherence of 99mTc EA thereby demonstrating that the receptors had greater affinity for immune complexes than for either antigen or antibody alone. The existence of membrane receptors on tumor-derived cells which react with the Fc portion of antigen-antibody complexes may provide an explanation for the mechanism by which immune complexes are capable of blocking cell-mediated tumor cell destruction irrespective of whether the receptors are on the tumor cells themselves or on admixed lymphocytes and macrophages."}
{"id": "PMID:1089729", "title": "Immune response to liposomal model membranes: restricted IgM and IgG anti-dinitrophenyl antibodies produced in guinea pigs.", "content": "Guinea pigs were immunized with liposomal model membranes actively sensitized with 2,4-dinitrophenyl-aminocaproylphosphatidyl-ethanolamine. The immune response was characterized by the formation of both IgM and IgG anti-DNP plaque-forming cells (PFC) which paralleled the appearance of anti-DNP antibodies in the serum. Plaque inhibition by DNP-lysine indicated that the PFC produced after liposomal immunization fell in a much narrower range of avidity groups than the PFC obtained after immunization with DNP-albumin and, in this regard, resembled MOPC 315. The restricted nature of the serum IgG anti-DNP antibodies was confirmed by isoelectric focusing and revealed the expression of a limited number of clones in all cases.", "contents": "Immune response to liposomal model membranes: restricted IgM and IgG anti-dinitrophenyl antibodies produced in guinea pigs. Guinea pigs were immunized with liposomal model membranes actively sensitized with 2,4-dinitrophenyl-aminocaproylphosphatidyl-ethanolamine. The immune response was characterized by the formation of both IgM and IgG anti-DNP plaque-forming cells (PFC) which paralleled the appearance of anti-DNP antibodies in the serum. Plaque inhibition by DNP-lysine indicated that the PFC produced after liposomal immunization fell in a much narrower range of avidity groups than the PFC obtained after immunization with DNP-albumin and, in this regard, resembled MOPC 315. The restricted nature of the serum IgG anti-DNP antibodies was confirmed by isoelectric focusing and revealed the expression of a limited number of clones in all cases."}
{"id": "PMID:1089740", "title": "Biological defense mechanisms. Evidence for the participation of superoxide in bacterial killing by xanthine oxidase.", "content": "Incubation of either Staphylococcus epidermidis or Escherichia coli with a sufficiently high concentration of xanthine oxidase, an enzyme capable of reducing oxygen to superoxide (O2-), resulted in the death of the microorganisms. Protection against the killing os S. epidermidis by xanthine oxidase was afforded by superoxide dismutase, an enzyme which converts O2- to O2 and H2O2, and also by catalase, which destroys H2O2. These findings indicate that neither O2- nor H2O2 were able to kill S. epidermidis under the experimental conditions, but that the bactericidal agent was the product of a reaction between O2- and H2O2. By contrast, E. coli was protected by catalase but not by superoxide dismutase. With this organism, therefore, H2O2 appears to have been the bactericidal agent.", "contents": "Biological defense mechanisms. Evidence for the participation of superoxide in bacterial killing by xanthine oxidase. Incubation of either Staphylococcus epidermidis or Escherichia coli with a sufficiently high concentration of xanthine oxidase, an enzyme capable of reducing oxygen to superoxide (O2-), resulted in the death of the microorganisms. Protection against the killing os S. epidermidis by xanthine oxidase was afforded by superoxide dismutase, an enzyme which converts O2- to O2 and H2O2, and also by catalase, which destroys H2O2. These findings indicate that neither O2- nor H2O2 were able to kill S. epidermidis under the experimental conditions, but that the bactericidal agent was the product of a reaction between O2- and H2O2. By contrast, E. coli was protected by catalase but not by superoxide dismutase. With this organism, therefore, H2O2 appears to have been the bactericidal agent."}
{"id": "PMID:1089741", "title": "The management of rhinoscleroma.", "content": "This investigation showed that the organism klebsiella rhinoscleromatis was present consistently in our cases. It was found to be difficult to eradicate completely although seemingly sensitive to common antibiotics in vitro. A prolonged treatment with bactericidal antibiotics is therefore necessary to eradicate it. Nasal dilatation rather than excision of granulations is required to restore nearly-normal physiological nasal function. Excision or cautery of granulations inside the nose is discouraged so as to avoid atropic changes.", "contents": "The management of rhinoscleroma. This investigation showed that the organism klebsiella rhinoscleromatis was present consistently in our cases. It was found to be difficult to eradicate completely although seemingly sensitive to common antibiotics in vitro. A prolonged treatment with bactericidal antibiotics is therefore necessary to eradicate it. Nasal dilatation rather than excision of granulations is required to restore nearly-normal physiological nasal function. Excision or cautery of granulations inside the nose is discouraged so as to avoid atropic changes."}
{"id": "PMID:1089743", "title": "The presence of IgA on the surface of rat thoractic duct lymphocytes which contain internal IgA.", "content": "The presence of lymphocytes with internal IgA among cells from rat thoracic duct lymph wdy. The number of cells detected was greater in animals kept in a convential animal house compared with those maintained under specific pathogen-free conditions. Thoracic duct lymph from B rats and adult thymectomized rats also contained cell with internal IgA. The surface Ig of the IgA-containing cells was studied using a double-labeling technique with (126I) anti-Ig to detect surface Ig, and fluorescein-conjugated anti-IgA  in large amounts, but very little IgM and no surface IgG2. The surface IgA was not acquired passively.", "contents": "The presence of IgA on the surface of rat thoractic duct lymphocytes which contain internal IgA. The presence of lymphocytes with internal IgA among cells from rat thoracic duct lymph wdy. The number of cells detected was greater in animals kept in a convential animal house compared with those maintained under specific pathogen-free conditions. Thoracic duct lymph from B rats and adult thymectomized rats also contained cell with internal IgA. The surface Ig of the IgA-containing cells was studied using a double-labeling technique with (126I) anti-Ig to detect surface Ig, and fluorescein-conjugated anti-IgA  in large amounts, but very little IgM and no surface IgG2. The surface IgA was not acquired passively."}
{"id": "PMID:1089744", "title": "Cross-reactivity between H-2K and H-2D products. I. Evidence for extensive and reciprocal serological cross-reactivity.", "content": "Serological cross-reactivity between the products of the H-2K and H-2D genes has been demonstrated by a design in which antibody was produced against determinants controlled by one locus (e .g . H-2K(k)), and then tested against the product of the opposite locus (e .g . H-2D(d)). A total of 13 out of 18 such test combinations exhibited H-2K-H-2D cross-reactivity. The presence or absence of cross-reactivity was reciprocal in most cases (i.e. antibody directed against the H-2K(k) gene product reacted with H-2(d) determinants, and antibody directed against the H-2D(d) gene product reacted with H-2K(k) determinants). An Ia-like reaction was detected with one antiserum which implied possible cross-reactivity between the products of two discrete la genes.", "contents": "Cross-reactivity between H-2K and H-2D products. I. Evidence for extensive and reciprocal serological cross-reactivity. Serological cross-reactivity between the products of the H-2K and H-2D genes has been demonstrated by a design in which antibody was produced against determinants controlled by one locus (e .g . H-2K(k)), and then tested against the product of the opposite locus (e .g . H-2D(d)). A total of 13 out of 18 such test combinations exhibited H-2K-H-2D cross-reactivity. The presence or absence of cross-reactivity was reciprocal in most cases (i.e. antibody directed against the H-2K(k) gene product reacted with H-2(d) determinants, and antibody directed against the H-2D(d) gene product reacted with H-2K(k) determinants). An Ia-like reaction was detected with one antiserum which implied possible cross-reactivity between the products of two discrete la genes."}
{"id": "PMID:1089745", "title": "Demonstration of an antibody-mediated tolerance state and its effect on antibody affinity.", "content": "We have described a model of immunological tolerance induced, in adult mice, by a single injection of a moderate dose of a hapten-protein conjugate. The data suggest that the mechanism of this tolerance state is the production of small amounts of high affinity antibody in response to the tolerance-inducing antigen injection. This antibody acts to inhibit the response to a subsequent challenge with antigen in complete Freund's adjuvant by a mechanism comparable to that of passive antibody-medicated immune suppression. It was shown that a small but high affinity. Tolerance was not terminated by transfer of normal syngeneic spleen or peritoneal cells into tolerant animals. Spleen cells from tolerant mice, when transferred into lethally irradiated, syngeneic animals, produced  a PFC response which is greater in magnitude and tolerance state had a significant degree of carrier specificity which was shown to be comparable to the carrier specificity of antibody-mediated immune suppression. hus, evidence was presented to show that one mechanism of tolerance in adult animals in the suppressive effect of small amounts of high affinity antibody formed in response to the tolerizing injection of antigen.", "contents": "Demonstration of an antibody-mediated tolerance state and its effect on antibody affinity. We have described a model of immunological tolerance induced, in adult mice, by a single injection of a moderate dose of a hapten-protein conjugate. The data suggest that the mechanism of this tolerance state is the production of small amounts of high affinity antibody in response to the tolerance-inducing antigen injection. This antibody acts to inhibit the response to a subsequent challenge with antigen in complete Freund's adjuvant by a mechanism comparable to that of passive antibody-medicated immune suppression. It was shown that a small but high affinity. Tolerance was not terminated by transfer of normal syngeneic spleen or peritoneal cells into tolerant animals. Spleen cells from tolerant mice, when transferred into lethally irradiated, syngeneic animals, produced  a PFC response which is greater in magnitude and tolerance state had a significant degree of carrier specificity which was shown to be comparable to the carrier specificity of antibody-mediated immune suppression. hus, evidence was presented to show that one mechanism of tolerance in adult animals in the suppressive effect of small amounts of high affinity antibody formed in response to the tolerizing injection of antigen."}
{"id": "PMID:1089746", "title": "Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products.", "content": "The multiplication of Toxoplasma gondii was quantitated in human monocytes in vitro by phase-contrast microscopy. Toxoplasma multiplication was identical in monocytes from subjects byt was significantly inhibited in cells from both sources if the monocytes were preincubated with immune lymphocytes and toxoplasma monocytes were preincubated with immune lymphocytes and toxoplasma antigen. Supernates prepared from toxoplasma-immune lymphocytes incubated with toxoplasma antigen were also effective in inducing in monocytes the capacity to inhibit toxoplasma multiplication. Supernative acitivty was evident after lymphocytes and antigen were incubated for as little as 15 min. The instruction of monocytes was also repid and reversible. Monocytes were fully induced to inhibit toxoplasma multiplication after a 2 h exposure to an active supernate, but they lost their inhibitory capacity on culture in vitro for 48 h in the absece of immune cells or their products. The lymphocytes particupating in the monocyte induction were identified as t cells. The in vitro stimulation of monocytes appeared to exhibit some specificity, since no inhibition of toxopreotein derivative and lymphocytes from tuberculin-positive subjects, concanavalin a-stimulated lymphocytes, or their supermates. Supernates which induced monocytes to inhibit toxoplasma multiplication did not influence parasite growth in HeLa cells.", "contents": "Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products. The multiplication of Toxoplasma gondii was quantitated in human monocytes in vitro by phase-contrast microscopy. Toxoplasma multiplication was identical in monocytes from subjects byt was significantly inhibited in cells from both sources if the monocytes were preincubated with immune lymphocytes and toxoplasma monocytes were preincubated with immune lymphocytes and toxoplasma antigen. Supernates prepared from toxoplasma-immune lymphocytes incubated with toxoplasma antigen were also effective in inducing in monocytes the capacity to inhibit toxoplasma multiplication. Supernative acitivty was evident after lymphocytes and antigen were incubated for as little as 15 min. The instruction of monocytes was also repid and reversible. Monocytes were fully induced to inhibit toxoplasma multiplication after a 2 h exposure to an active supernate, but they lost their inhibitory capacity on culture in vitro for 48 h in the absece of immune cells or their products. The lymphocytes particupating in the monocyte induction were identified as t cells. The in vitro stimulation of monocytes appeared to exhibit some specificity, since no inhibition of toxopreotein derivative and lymphocytes from tuberculin-positive subjects, concanavalin a-stimulated lymphocytes, or their supermates. Supernates which induced monocytes to inhibit toxoplasma multiplication did not influence parasite growth in HeLa cells."}
{"id": "PMID:1089747", "title": "Scanning electron microscopy of tobacco mosaic virus-labeled lymphocyte surface antigens.", "content": "The study of surface antigen by immunoelectron microscopy has been hampered by the fact that thin sections of cells provide only a view of the cell perimeter in an essentially two- dimensional fashion. Although the reconstruction of the entire cell from serial sections has been accomplished (1), it remains too exacting a technique and will find only exceptional application. Carbon-platinum replicas (2) allow the inspection of larger surface areas and therefore are better suited for studying the distribution of antigens (3). But since only relatively smooth surfaces will yield stable replicas, cells with large numbers of microvilli are not amenable to this technique. Despire its limited resolution, scanning electron microscopy (SEM) seems to be the method of choice because it can provide a view of almost half of the surface of a cell close to its natural configuration, particularly after critical point or freeze drying (4, 5). Immunological-labeling methods have not yet been routinely applied to SEM although both latex spheres (6) and hemocyanin (7) have been used with some success. The optimal visual marker should possess the following properties: be of a distinctive shape, chemically stable, and have per se a low binding affinity for cell surfaces. Tobacco mosaic virus (TMV), a marker with which we are familiar in transmission electron microscopy (8), seems to meet these demands; it has rod-like shape and defined dimensions (15 x 300 nm) and in addition it can easily be distinguished from surface microvilli. As the hybrid antibody technique (9) is also applicable to TMV, we have attempted to combine such immunological labeling with SEM. We present evidence that surface antigens can indeed be visualized by SEM, using the TMV marker in conjunction with the hybrid antibody technique.", "contents": "Scanning electron microscopy of tobacco mosaic virus-labeled lymphocyte surface antigens. The study of surface antigen by immunoelectron microscopy has been hampered by the fact that thin sections of cells provide only a view of the cell perimeter in an essentially two- dimensional fashion. Although the reconstruction of the entire cell from serial sections has been accomplished (1), it remains too exacting a technique and will find only exceptional application. Carbon-platinum replicas (2) allow the inspection of larger surface areas and therefore are better suited for studying the distribution of antigens (3). But since only relatively smooth surfaces will yield stable replicas, cells with large numbers of microvilli are not amenable to this technique. Despire its limited resolution, scanning electron microscopy (SEM) seems to be the method of choice because it can provide a view of almost half of the surface of a cell close to its natural configuration, particularly after critical point or freeze drying (4, 5). Immunological-labeling methods have not yet been routinely applied to SEM although both latex spheres (6) and hemocyanin (7) have been used with some success. The optimal visual marker should possess the following properties: be of a distinctive shape, chemically stable, and have per se a low binding affinity for cell surfaces. Tobacco mosaic virus (TMV), a marker with which we are familiar in transmission electron microscopy (8), seems to meet these demands; it has rod-like shape and defined dimensions (15 x 300 nm) and in addition it can easily be distinguished from surface microvilli. As the hybrid antibody technique (9) is also applicable to TMV, we have attempted to combine such immunological labeling with SEM. We present evidence that surface antigens can indeed be visualized by SEM, using the TMV marker in conjunction with the hybrid antibody technique."}
{"id": "PMID:1089748", "title": "Torulopsis bacarum, Torulopsis pustula and Torulopsis multis-gemmis sp. nov., three new yeasts from soft fruit.", "content": "Three new species of yeasts isolated from fresh soft fruit are described. Torulopsis pustula was isolated from blackcurrants, Torulopsis bacarum from blackcurrants, strawberries and raspberries, and Torulopsis multis-gemmis from raspberries. All differ from known species in the test substrates they utilize. In addition, cells of Torulopsis pustula often form a bulbous projection near their apices from which budding takes place. These buds are frequently pear- or heart-shaped.", "contents": "Torulopsis bacarum, Torulopsis pustula and Torulopsis multis-gemmis sp. nov., three new yeasts from soft fruit. Three new species of yeasts isolated from fresh soft fruit are described. Torulopsis pustula was isolated from blackcurrants, Torulopsis bacarum from blackcurrants, strawberries and raspberries, and Torulopsis multis-gemmis from raspberries. All differ from known species in the test substrates they utilize. In addition, cells of Torulopsis pustula often form a bulbous projection near their apices from which budding takes place. These buds are frequently pear- or heart-shaped."}
{"id": "PMID:1089749", "title": "Thermosensitive production of their transfer systems by group S plasmids.", "content": "Transfer of plasmids of group S is much more efficient at low temperatures (e.g. 22 degrees C) than at 37 degrees C. This is due to failure of the donor strain to produce the transfer system during growth at the higher temperature.", "contents": "Thermosensitive production of their transfer systems by group S plasmids. Transfer of plasmids of group S is much more efficient at low temperatures (e.g. 22 degrees C) than at 37 degrees C. This is due to failure of the donor strain to produce the transfer system during growth at the higher temperature."}
{"id": "PMID:1089750", "title": "Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans.", "content": "It has been possible to induce the yeast-mycelium transformation in Candida albicans by growth of the organism under completely defined conditions in batch culture. Protoplasts have been obtained from the two forms by using a lytic enzyme preparation from Streptomyces violaceus. A plasma membrane fraction was prepared by osmotic lysis of these protoplasts and fractionated by using a combination of differential and discontinuous sucrose density-gradient flotation centrifugation. The purity of this fraction was determined by radioactive dansylation and iodination of plasma membranes of intact protoplasts followed by localization of the radioactivity upon fractionation. This procedure demonstrated less than 4% contamination of the plasma membrane fraction with other cell membranes. Chemical analysis of this fraction revealed that the major components were protein and lipid. Membranes from the yeast form contained (w/w): 50% protein, 45% lipid, 9% carbohydrate and 0.3% nucleic acid. Plasma membranes from the mycelial form contained significantly more carbohydrate and were found to be composed of (w/w): 43% protein, 31% lipid, 25% carbohydrate and 0.5% nucleic acid. Marked differences were also observed between the phospholipid, free and esterified sterols, and total fatty acids of membranes from the two forms of the organism.", "contents": "Isolation and chemical characterization of plasma membranes from the yeast and mycelial forms of Candida albicans. It has been possible to induce the yeast-mycelium transformation in Candida albicans by growth of the organism under completely defined conditions in batch culture. Protoplasts have been obtained from the two forms by using a lytic enzyme preparation from Streptomyces violaceus. A plasma membrane fraction was prepared by osmotic lysis of these protoplasts and fractionated by using a combination of differential and discontinuous sucrose density-gradient flotation centrifugation. The purity of this fraction was determined by radioactive dansylation and iodination of plasma membranes of intact protoplasts followed by localization of the radioactivity upon fractionation. This procedure demonstrated less than 4% contamination of the plasma membrane fraction with other cell membranes. Chemical analysis of this fraction revealed that the major components were protein and lipid. Membranes from the yeast form contained (w/w): 50% protein, 45% lipid, 9% carbohydrate and 0.3% nucleic acid. Plasma membranes from the mycelial form contained significantly more carbohydrate and were found to be composed of (w/w): 43% protein, 31% lipid, 25% carbohydrate and 0.5% nucleic acid. Marked differences were also observed between the phospholipid, free and esterified sterols, and total fatty acids of membranes from the two forms of the organism."}
{"id": "PMID:1089751", "title": "Chromosome transfer in Proteus mirabilis mediated by hybrid plasmid.", "content": "A previously-described fused plasmid, P-lacRIdrd19, was found to mediate chromosomal transfer between cells of Proteus mirabilis strain PM5006; PM5006-(P-lacRIdrd19) was usually the donor and various auxotrophs of PM5006 resistant to nalidixic acid and/or streptomycin were recipients. The donor was usually counterselected with nalidixic acid and/or high concentrations of streptomycin. Recombination experiments with single markers indicated a 40-fold variation in recombination frequencies for different markers. Mapping double-auxotrophic markers by their gradient of transmission confirmed this variation and placed each of two independent isolates of eight markers in a linkage group his-ser-ura-pyrB-trp-cys-ade-ilv. Some donor markers did not register. Despite low recombination frequencies, interrupted mating experiments showed a polarity of early marker transfer. The segregation of unselected markers confirmed the order of some markers and showed that genetic material passed from the presumptive donor to the recipient. Recipients with two auxotrophic markers which could not be cotransduced by phage 5006M were converted to prototrophy by conjugation. The plasmid transferred to recipients at high frequency and all recombinants carried it. Recombinants could act as donors in further matings. Recombinants were fully susceptible to phage 5006M, unlike transductants of PM5006 by this phage. Direct involvement of the plasmid was indicated by drastically diminished recombination frequencies in crosses with recipients carrying P-lac as resident. P-lac had previously been shown to reduce the frequency of transfer of the hybrid plasmid to cells harbouring it. The histidine region was the first to register in recipients and recombined at the highest frequency of 5 times 10-6/donor cell. Some temporary association of plasmid and perhaps only the histidine region of the chromosome is favoured as the mechanism of chromosomal transfer. This could explain why not all donor markers could be mapped. Transduction and transformation were excluded as the cause of results.", "contents": "Chromosome transfer in Proteus mirabilis mediated by hybrid plasmid. A previously-described fused plasmid, P-lacRIdrd19, was found to mediate chromosomal transfer between cells of Proteus mirabilis strain PM5006; PM5006-(P-lacRIdrd19) was usually the donor and various auxotrophs of PM5006 resistant to nalidixic acid and/or streptomycin were recipients. The donor was usually counterselected with nalidixic acid and/or high concentrations of streptomycin. Recombination experiments with single markers indicated a 40-fold variation in recombination frequencies for different markers. Mapping double-auxotrophic markers by their gradient of transmission confirmed this variation and placed each of two independent isolates of eight markers in a linkage group his-ser-ura-pyrB-trp-cys-ade-ilv. Some donor markers did not register. Despite low recombination frequencies, interrupted mating experiments showed a polarity of early marker transfer. The segregation of unselected markers confirmed the order of some markers and showed that genetic material passed from the presumptive donor to the recipient. Recipients with two auxotrophic markers which could not be cotransduced by phage 5006M were converted to prototrophy by conjugation. The plasmid transferred to recipients at high frequency and all recombinants carried it. Recombinants could act as donors in further matings. Recombinants were fully susceptible to phage 5006M, unlike transductants of PM5006 by this phage. Direct involvement of the plasmid was indicated by drastically diminished recombination frequencies in crosses with recipients carrying P-lac as resident. P-lac had previously been shown to reduce the frequency of transfer of the hybrid plasmid to cells harbouring it. The histidine region was the first to register in recipients and recombined at the highest frequency of 5 times 10-6/donor cell. Some temporary association of plasmid and perhaps only the histidine region of the chromosome is favoured as the mechanism of chromosomal transfer. This could explain why not all donor markers could be mapped. Transduction and transformation were excluded as the cause of results."}
{"id": "PMID:1089752", "title": "Mutations which affect amino acid transport in Aspergillus nidulans.", "content": "Mutants deficient in amino acid transport (aau), and unable to utilize L-glutamate as a sole carbon and nitrogen source, have been isolated. There are four unlinked genes involved: aauA, aauB, aauC and aauD. The transport levels of certain amino acids, and the growth characteristics on certain nitrogen and carbon sources and toxic amino acid analogues, indicate that: aauAI has defective transport of acidic amino acids; aauBI has defective transport of acidic and neutral amino acids; aauCI and aauDI have defective transport of acid, neutral and aromatic amino acids. aauAI and aauBI are recessive for all three characteristics in the heterozygous diploid; aauCI and aauDI are dominant.", "contents": "Mutations which affect amino acid transport in Aspergillus nidulans. Mutants deficient in amino acid transport (aau), and unable to utilize L-glutamate as a sole carbon and nitrogen source, have been isolated. There are four unlinked genes involved: aauA, aauB, aauC and aauD. The transport levels of certain amino acids, and the growth characteristics on certain nitrogen and carbon sources and toxic amino acid analogues, indicate that: aauAI has defective transport of acidic amino acids; aauBI has defective transport of acidic and neutral amino acids; aauCI and aauDI have defective transport of acid, neutral and aromatic amino acids. aauAI and aauBI are recessive for all three characteristics in the heterozygous diploid; aauCI and aauDI are dominant."}
{"id": "PMID:1089755", "title": "Sub-cellular location of mercury in yeast grown in the presence of mercuric chloride.", "content": "The distribution of 203Hg in Saccharomyces cerevisiae grown in the presence of mercuric chloride has been examined by physical and chemical fractionation procedures and autoradiography. The major fraction of the bound mercury is tightly bound to the wall. A significant quantity of mercury penetrates to the cytoplasm but only a minor fraction is present as low molecular weight components. The wall associated mercury is not readily released by extraction with sodium hydroxide or ethylenediamine but a major fraction is solubilized by pronase and Helicase treatment. Isolated walls are capable of binding their own weight of mercury to high-affinity adsorption sites. The major role of the cell envelope in the in vivo binding of mercury and the penetration to the cytoplasm of mercury was confirmed by autoradiography.", "contents": "Sub-cellular location of mercury in yeast grown in the presence of mercuric chloride. The distribution of 203Hg in Saccharomyces cerevisiae grown in the presence of mercuric chloride has been examined by physical and chemical fractionation procedures and autoradiography. The major fraction of the bound mercury is tightly bound to the wall. A significant quantity of mercury penetrates to the cytoplasm but only a minor fraction is present as low molecular weight components. The wall associated mercury is not readily released by extraction with sodium hydroxide or ethylenediamine but a major fraction is solubilized by pronase and Helicase treatment. Isolated walls are capable of binding their own weight of mercury to high-affinity adsorption sites. The major role of the cell envelope in the in vivo binding of mercury and the penetration to the cytoplasm of mercury was confirmed by autoradiography."}
{"id": "PMID:1089756", "title": "R factors from Serratia marcescens.", "content": "In recent years, Serratia marcescens has become established in certain localities as an agent of hospital infection and cross-infection (Clayton & von Graevenitz, 1966; Wilfert, Barrett & Kass, 1968; Davis, Foltz & Blakemore, 1970; Wilkowske, Washington, Martin & Ritts, 1970). In general, strains of S. marcescens isolated from infective lesions differ from those from other sources in being non-pigmented and antibiotic resistant (Ewing, Johnson & David, 1962; Clayton & von Graevenitz, 1966). Medeiros & O'Brien (1969) and Schaefler et al. (1971) described strains of S. marcescens, isolated from hospital patients, which were able to transfer R factors to Escherichia coli. Transfer of resistance to E. coli has also been reported from strains of S. marcescens isolated in France (Grimont & Dulong de Rosnay, 1972; Scavizzi, 1972; Lemosquet-Villemon, Morel & Freymuth, 1973)9 We have collected strains of S. marcescens, most, but not all, clinical isolates, from widely separate geographical areas; each strain was tested for antibiotic resistance and for R factors transmissible to E. coli K129 The R factors were classified by compatibility in K12 (Datta, 1974). Our purpose was to find out how much of the antibiotic resistance observed in S. marcescens is characteristic of that genus and to what extent it is shared with other bacterial genera (Coetzee, Datta & Hedges, 1972; Datta & Hedges, 1972a; Hedges, 1974). The R factors described by Medeiros & O'Brien (1969) and Lemosquet-Villemon et al. (1973) were included in this study.", "contents": "R factors from Serratia marcescens. In recent years, Serratia marcescens has become established in certain localities as an agent of hospital infection and cross-infection (Clayton & von Graevenitz, 1966; Wilfert, Barrett & Kass, 1968; Davis, Foltz & Blakemore, 1970; Wilkowske, Washington, Martin & Ritts, 1970). In general, strains of S. marcescens isolated from infective lesions differ from those from other sources in being non-pigmented and antibiotic resistant (Ewing, Johnson & David, 1962; Clayton & von Graevenitz, 1966). Medeiros & O'Brien (1969) and Schaefler et al. (1971) described strains of S. marcescens, isolated from hospital patients, which were able to transfer R factors to Escherichia coli. Transfer of resistance to E. coli has also been reported from strains of S. marcescens isolated in France (Grimont & Dulong de Rosnay, 1972; Scavizzi, 1972; Lemosquet-Villemon, Morel & Freymuth, 1973)9 We have collected strains of S. marcescens, most, but not all, clinical isolates, from widely separate geographical areas; each strain was tested for antibiotic resistance and for R factors transmissible to E. coli K129 The R factors were classified by compatibility in K12 (Datta, 1974). Our purpose was to find out how much of the antibiotic resistance observed in S. marcescens is characteristic of that genus and to what extent it is shared with other bacterial genera (Coetzee, Datta & Hedges, 1972; Datta & Hedges, 1972a; Hedges, 1974). The R factors described by Medeiros & O'Brien (1969) and Lemosquet-Villemon et al. (1973) were included in this study."}
{"id": "PMID:1089757", "title": "Sphere-rod morphogenesis of Escherichia coli.", "content": "The morphogenetic capacity of E. coli was studied by converting the rod-shaped cells into spheres and then determining whether these spheres could revert to rods. The morphogenesis of cells was followed by immobilizing them in a viscous Methocel-containing medium. Two different types of spheres were prepared: cells which retained a mechanically intact sacculus, and osmotically sensitive sphaeroplasts lacking a sacculus. The sphaeroplasts were not able to revert to rods although they were able to synthesize a new sacculus. In contrast, spheres which had retained an intact sacculus were able to reshape themselves into rods. The were also able to form new ends at (or near) the sites of the ends on the original rods.", "contents": "Sphere-rod morphogenesis of Escherichia coli. The morphogenetic capacity of E. coli was studied by converting the rod-shaped cells into spheres and then determining whether these spheres could revert to rods. The morphogenesis of cells was followed by immobilizing them in a viscous Methocel-containing medium. Two different types of spheres were prepared: cells which retained a mechanically intact sacculus, and osmotically sensitive sphaeroplasts lacking a sacculus. The sphaeroplasts were not able to revert to rods although they were able to synthesize a new sacculus. In contrast, spheres which had retained an intact sacculus were able to reshape themselves into rods. The were also able to form new ends at (or near) the sites of the ends on the original rods."}
{"id": "PMID:1089758", "title": "Characterization of the growth of Pseudomonas putida LP on lipoate and its analogues: transport, oxidation, sulphur source, and enzyme induction.", "content": "Pseudomonas putida LP, which grows on lipoate, NH4NO3 and mineral salts, converts most of the organic substrate to bisnor-lipoate (1,2-dithiolane-3-propanoic acid) and acetyl-CoA. D-, L-, or DL-lipoate serve equally well as carbon and sulphur sources. There was no growth on or bacterial oxidation of the chemically synthesized bisnor- or tetranor-(1,2-dithiolane-3-carboxylic acid) chain-shortened analogues, but these, as well as lipoate, could supply the sulphur needed for growth when acetate was provided as the sole source of carbon. The uptake of lipoate by the bacterium is very slow and non-inducible, while the uptake of acetate is faster than octanoate. The oxidation of octanoate is more rapid and extensive than that of lipoate. Levels of acyl-CoA synthetase are not affected by the source of carbon, but activities of isocitrate lyase and malate synthase are higher when the cells are grown in acetate, octanoate or lipoate and lower when glucose is the carbon source. The glyoxylate cycle is induced to facilitate utilization of acetyl-CoA derived from lipoate, which is also degraded to water-soluble catabolites that yield the much smaller amount of sulphur required for growth.", "contents": "Characterization of the growth of Pseudomonas putida LP on lipoate and its analogues: transport, oxidation, sulphur source, and enzyme induction. Pseudomonas putida LP, which grows on lipoate, NH4NO3 and mineral salts, converts most of the organic substrate to bisnor-lipoate (1,2-dithiolane-3-propanoic acid) and acetyl-CoA. D-, L-, or DL-lipoate serve equally well as carbon and sulphur sources. There was no growth on or bacterial oxidation of the chemically synthesized bisnor- or tetranor-(1,2-dithiolane-3-carboxylic acid) chain-shortened analogues, but these, as well as lipoate, could supply the sulphur needed for growth when acetate was provided as the sole source of carbon. The uptake of lipoate by the bacterium is very slow and non-inducible, while the uptake of acetate is faster than octanoate. The oxidation of octanoate is more rapid and extensive than that of lipoate. Levels of acyl-CoA synthetase are not affected by the source of carbon, but activities of isocitrate lyase and malate synthase are higher when the cells are grown in acetate, octanoate or lipoate and lower when glucose is the carbon source. The glyoxylate cycle is induced to facilitate utilization of acetyl-CoA derived from lipoate, which is also degraded to water-soluble catabolites that yield the much smaller amount of sulphur required for growth."}
{"id": "PMID:1089759", "title": "Polarity of the cysJIH operon of Salmonella typhimurium.", "content": "Certain point and deletion mutants with lesions in the cysJ gene of Salmonella typhimurium have low levels of enzymes coded by the cysI and cysH genes. These results support the hypothesis that an operon exists comprising genes cysJ, I and H which is transcribed in the direction from cysJ to H. The nearby cysC and cysD genes do not form part of this cysJIH operon.", "contents": "Polarity of the cysJIH operon of Salmonella typhimurium. Certain point and deletion mutants with lesions in the cysJ gene of Salmonella typhimurium have low levels of enzymes coded by the cysI and cysH genes. These results support the hypothesis that an operon exists comprising genes cysJ, I and H which is transcribed in the direction from cysJ to H. The nearby cysC and cysD genes do not form part of this cysJIH operon."}
{"id": "PMID:1089763", "title": "Some morphometric methods for the central nervous system.", "content": "It is the quantitative study of the central nervous system to enumerate neurone populations. Since neurones are generally arranged in non-random patterns the conventional method of counting with a square lattice graticule in the ocular of the microscope is severely restricted. Appropriately tailored methods of (a) counting, or (b) estimating the density of a neurone population must therefore be used. Four types of neuronal distribution pattern are discussed and morphometric methods adapted to them are presented, as follows: (1) Where the whole population is circumscribed, as in the anterior horn motor neurones of the cord, direct counting is feasible. Only those cells whose nucleoli are visible should be included; this diminishes the bias in favour of large cells, and if one corrects for the fact that larger neurones have larger nucleoli the bias can be eliminated completely. (2) Where neurones are situated on an \"interface\", as Purkinje cells are, the length of its profile (in this instance the boundary between the molecular and granular zones) is estimated by superimposing parallel lines on the microscopical image and counting intersections as these lines cross the interface. The number of Purkinje cells in each particular field is counted at the same time. A \"linear dinsity\" (PL) is then calculated from the formula pl = 2n/pid where n is the quotient of (a) the number of cells counted along (b) an estimated length of profile, and d is the distance between the parallel lines of the graticule. For comparative studies between cerebella of different sizes a correction factor is easily introduced. Those who prefer to work on photomicrographs can use a mapping wheel to measure a length of profile and then proceed as before. (3) Where neurones are organised in a curving band, as in the dentate nucleus, another form of cell density can be established. The microscope is focused on a neurone at random, and the distance from this cell to its nearest neighbour is measured, preferably by means of a screw-micrometer eyepiece. The mean value r of a number of such measurements is substituted in a formula which gives the \"areal density\" of the neurone population: pa = 1/4r2. This method can be applied to photomicrographs by using a pair of dividers and/or an accurate rule to measure the set of r values. (4) In large collections of neurones, e.g., in the thalamus, three methods are available: (a) the nearest neighbour method; (b) a conventional squared graticule count, and (c) a count of cells intersected by a line probe as in Haug's (1972) technique (fig. 5), or a modified form of Strong's (1966) transect method.", "contents": "Some morphometric methods for the central nervous system. It is the quantitative study of the central nervous system to enumerate neurone populations. Since neurones are generally arranged in non-random patterns the conventional method of counting with a square lattice graticule in the ocular of the microscope is severely restricted. Appropriately tailored methods of (a) counting, or (b) estimating the density of a neurone population must therefore be used. Four types of neuronal distribution pattern are discussed and morphometric methods adapted to them are presented, as follows: (1) Where the whole population is circumscribed, as in the anterior horn motor neurones of the cord, direct counting is feasible. Only those cells whose nucleoli are visible should be included; this diminishes the bias in favour of large cells, and if one corrects for the fact that larger neurones have larger nucleoli the bias can be eliminated completely. (2) Where neurones are situated on an \"interface\", as Purkinje cells are, the length of its profile (in this instance the boundary between the molecular and granular zones) is estimated by superimposing parallel lines on the microscopical image and counting intersections as these lines cross the interface. The number of Purkinje cells in each particular field is counted at the same time. A \"linear dinsity\" (PL) is then calculated from the formula pl = 2n/pid where n is the quotient of (a) the number of cells counted along (b) an estimated length of profile, and d is the distance between the parallel lines of the graticule. For comparative studies between cerebella of different sizes a correction factor is easily introduced. Those who prefer to work on photomicrographs can use a mapping wheel to measure a length of profile and then proceed as before. (3) Where neurones are organised in a curving band, as in the dentate nucleus, another form of cell density can be established. The microscope is focused on a neurone at random, and the distance from this cell to its nearest neighbour is measured, preferably by means of a screw-micrometer eyepiece. The mean value r of a number of such measurements is substituted in a formula which gives the \"areal density\" of the neurone population: pa = 1/4r2. This method can be applied to photomicrographs by using a pair of dividers and/or an accurate rule to measure the set of r values. (4) In large collections of neurones, e.g., in the thalamus, three methods are available: (a) the nearest neighbour method; (b) a conventional squared graticule count, and (c) a count of cells intersected by a line probe as in Haug's (1972) technique (fig. 5), or a modified form of Strong's (1966) transect method."}
{"id": "PMID:1089765", "title": "Immunohistochemical demonstration of antigen associated with the post-synaptic lattice.", "content": "The binding of amtosera against synaptosomes from rat cerebral cortex to dissociated cells from both cerebral cortex and liver was assessed by immunofluorescent labelling. This showed that none of the liver cells bound antisynaptosome antibodies but that some of the cerebral cortex cells bound antibody on their surfaces. Immunofluorescent labelling showed that all the particles in the crude mitochondrial fraction from rat cortex bound antibodies present in the unadsorbed antisera. However, when the antisera were absorbed with purified mitochondria and myelin, only a proportion of the mitochondrial fraction particles then bound antibody. Isolated IgG from the adsorbed antisera was labelled with ferritin and incubated with the crude mitochondrial fraction. Examination in the electron microscope showed that the ferritin and hence the antisynaptosome antibody was bound to the post-synaptic thickenings of about 20% of synaptosomes having their junctions in the plane of section.", "contents": "Immunohistochemical demonstration of antigen associated with the post-synaptic lattice. The binding of amtosera against synaptosomes from rat cerebral cortex to dissociated cells from both cerebral cortex and liver was assessed by immunofluorescent labelling. This showed that none of the liver cells bound antisynaptosome antibodies but that some of the cerebral cortex cells bound antibody on their surfaces. Immunofluorescent labelling showed that all the particles in the crude mitochondrial fraction from rat cortex bound antibodies present in the unadsorbed antisera. However, when the antisera were absorbed with purified mitochondria and myelin, only a proportion of the mitochondrial fraction particles then bound antibody. Isolated IgG from the adsorbed antisera was labelled with ferritin and incubated with the crude mitochondrial fraction. Examination in the electron microscope showed that the ferritin and hence the antisynaptosome antibody was bound to the post-synaptic thickenings of about 20% of synaptosomes having their junctions in the plane of section."}
{"id": "PMID:1089766", "title": "Review of basic concepts of cell kinetics as applied to brain tumors.", "content": "The authors review and discuss the basic concepts of cell kinetics as applied to brain tumors. Uncontrolled growth of a neoplasm represents an expanding tumor cell population. Four growth parameters characterize the behavior of a neoplastic population: cell cycle time, growth fraction, tumor doubling time, and cell loss. The concept of provisionally nondividing cells explains the disparity between cell cycle time and tumor doubling time. Human gliomas, like many non-neural solid tumors, contain variable proportions of actively proliferating and nonproliferating tumor cells; this ratio is expressed by the growth fraction. The major kinetic difference between glioblastomas and differentiated astrocytomas resides in their respective growth fractions, in all likelihood an inherent biological characteristic of each tumor. Glioblastoma proliferates at a rapid rate, and only a high rate of cell loss prevents this tumor from doubling its volume in less than 1 week. The selection of drugs and design of drug schedules for treatment of glioblastomas should be made with the knowledge that 60% to 70% of the cells in this tumor are resting (nonproliferating). If experience with other solid tumors is any guide, judicious selection and combined use of drugs according to kinetically sound schedules will produce more effective chemotherapy of brain tumors.", "contents": "Review of basic concepts of cell kinetics as applied to brain tumors. The authors review and discuss the basic concepts of cell kinetics as applied to brain tumors. Uncontrolled growth of a neoplasm represents an expanding tumor cell population. Four growth parameters characterize the behavior of a neoplastic population: cell cycle time, growth fraction, tumor doubling time, and cell loss. The concept of provisionally nondividing cells explains the disparity between cell cycle time and tumor doubling time. Human gliomas, like many non-neural solid tumors, contain variable proportions of actively proliferating and nonproliferating tumor cells; this ratio is expressed by the growth fraction. The major kinetic difference between glioblastomas and differentiated astrocytomas resides in their respective growth fractions, in all likelihood an inherent biological characteristic of each tumor. Glioblastoma proliferates at a rapid rate, and only a high rate of cell loss prevents this tumor from doubling its volume in less than 1 week. The selection of drugs and design of drug schedules for treatment of glioblastomas should be made with the knowledge that 60% to 70% of the cells in this tumor are resting (nonproliferating). If experience with other solid tumors is any guide, judicious selection and combined use of drugs according to kinetically sound schedules will produce more effective chemotherapy of brain tumors."}
{"id": "PMID:1089769", "title": "Gallium-67-citrate scanning for the localization and staging of lymphomas.", "content": "One-hundred-eight 67Ga-citrate scans were evaluated in Hodgkin's and non-Hodgkin's lymphomas for staging, diagnosis of recurrence, and for determing the results of treatment. Accuracy as confirmed by comparing the scans with pathologic material or roentgenologic and clinical findings was found to be 83%. Known lymph node involvement was diagnosed correctly in 87% but accuracy was only 48% for extranodal areas. Bone lesions were diagnosed correctly in 83% compared with only 48% of lesions of the lungs and liver. Scans were 75% accurate in 28 patients scanned for initial staging. False negatives were present in 12%; recurrent tumor in an area of prior radiation therapy appeared to be the most common cause. There were 5% false positives. Gallium scanning is a useful adjunct to other methods of detecting lymphoma.", "contents": "Gallium-67-citrate scanning for the localization and staging of lymphomas. One-hundred-eight 67Ga-citrate scans were evaluated in Hodgkin's and non-Hodgkin's lymphomas for staging, diagnosis of recurrence, and for determing the results of treatment. Accuracy as confirmed by comparing the scans with pathologic material or roentgenologic and clinical findings was found to be 83%. Known lymph node involvement was diagnosed correctly in 87% but accuracy was only 48% for extranodal areas. Bone lesions were diagnosed correctly in 83% compared with only 48% of lesions of the lungs and liver. Scans were 75% accurate in 28 patients scanned for initial staging. False negatives were present in 12%; recurrent tumor in an area of prior radiation therapy appeared to be the most common cause. There were 5% false positives. Gallium scanning is a useful adjunct to other methods of detecting lymphoma."}
{"id": "PMID:1089770", "title": "A rapid method for evaluation of regional cerebral blood flow after intra-arterial injection of 133Xe.", "content": "A technique based on the stochastic method is described for the computation of regional blood flow (RBF) after intra-arterial infection of 133Xe. Results are presented on scintigrams, defined by 600-800 channels in a 64 times 64 matrix. The content of each channel is equal to the computed RBF value. The standard deviation is also computed for each channel. The total computation cycle is achieved within 10 min. The method has been used to investigate the influence of anesthesia or drugs on regional blood flow. For this purpose, a differential printout program was written in which variations of RBF values between the two measurements are expressed in units of a fixed percentage; provision is made for calculation of RBF variations in units of differential standard deviations. Data are in good accordance with other clinical findings. This method is suitable for clinical use when blood flow values in gray and white substances are not requested.", "contents": "A rapid method for evaluation of regional cerebral blood flow after intra-arterial injection of 133Xe. A technique based on the stochastic method is described for the computation of regional blood flow (RBF) after intra-arterial infection of 133Xe. Results are presented on scintigrams, defined by 600-800 channels in a 64 times 64 matrix. The content of each channel is equal to the computed RBF value. The standard deviation is also computed for each channel. The total computation cycle is achieved within 10 min. The method has been used to investigate the influence of anesthesia or drugs on regional blood flow. For this purpose, a differential printout program was written in which variations of RBF values between the two measurements are expressed in units of a fixed percentage; provision is made for calculation of RBF variations in units of differential standard deviations. Data are in good accordance with other clinical findings. This method is suitable for clinical use when blood flow values in gray and white substances are not requested."}
{"id": "PMID:1089771", "title": "Evaluation of portable radionuclide method for measurement of left ventricular ejection fraction and cardiac output.", "content": "Seventeen patients with coronary artery, valvular, or myopathic heart disease were studied to determine correlations of the cardiac output and ejection fraction when comparing the results obtained with a portable probe technique using 113mIn with those obtained with standard methods (cineangiographic, Fick, and dye dilution). With ejection fractions ranging from o.10 to 0.85, the coefficient of correlation was 0.90 when comparing cineangiographic and radionuclide techniques. Cardiac output determinations by the radionuclide technique also correlated well with standard methods (r equals 0.88). The radionuclide method shows promise as an accurate, safe, and simple method in the evaluation of cardiac function at the bedside.", "contents": "Evaluation of portable radionuclide method for measurement of left ventricular ejection fraction and cardiac output. Seventeen patients with coronary artery, valvular, or myopathic heart disease were studied to determine correlations of the cardiac output and ejection fraction when comparing the results obtained with a portable probe technique using 113mIn with those obtained with standard methods (cineangiographic, Fick, and dye dilution). With ejection fractions ranging from o.10 to 0.85, the coefficient of correlation was 0.90 when comparing cineangiographic and radionuclide techniques. Cardiac output determinations by the radionuclide technique also correlated well with standard methods (r equals 0.88). The radionuclide method shows promise as an accurate, safe, and simple method in the evaluation of cardiac function at the bedside."}
{"id": "PMID:1089772", "title": "Reconstruction of the Fresnel-coded gamma camera images by digital computer.", "content": "An algorithm for the digital reconstruction of the Fresnel-coded gamma camera image consists of the spatial Fourier transform of the hologram after applying a quadratic phase shift. Background subtraction is implemented by use of positive and negative zone plates. Tomographic planes are reconstructed in 27 sec from a single zone-plate shadow image by successively varying a parameter in the reconstruction algorithm on a small computer system.", "contents": "Reconstruction of the Fresnel-coded gamma camera images by digital computer. An algorithm for the digital reconstruction of the Fresnel-coded gamma camera image consists of the spatial Fourier transform of the hologram after applying a quadratic phase shift. Background subtraction is implemented by use of positive and negative zone plates. Tomographic planes are reconstructed in 27 sec from a single zone-plate shadow image by successively varying a parameter in the reconstruction algorithm on a small computer system."}
{"id": "PMID:1089774", "title": "An experimental study of autoclaved autogenous mandible in combination with cancellous bone and marrow.", "content": "Results of a study in eight dogs indicate that a combination of cancellous bone and marrow contained within autoclaved bone can produce a successful graft.  Four implants failed because of breakdown of the oral mucosa and exposure of the graft; rejection may have been the cause of the breakdown in one instance.  Although most of the factors that caused exposure of the grafts probably could be controlled in a clinical situation, the technique cannot be recommended without reservation.", "contents": "An experimental study of autoclaved autogenous mandible in combination with cancellous bone and marrow. Results of a study in eight dogs indicate that a combination of cancellous bone and marrow contained within autoclaved bone can produce a successful graft.  Four implants failed because of breakdown of the oral mucosa and exposure of the graft; rejection may have been the cause of the breakdown in one instance.  Although most of the factors that caused exposure of the grafts probably could be controlled in a clinical situation, the technique cannot be recommended without reservation."}
{"id": "PMID:1089775", "title": "Hepatitis B in a pediatric hemodialysis unit.", "content": "Serial HB Ag determinations were obtained on 62 children undergoing hemodialysis during a 31/2-year period. Thirty six (58%) of the patients had atleast one positive HB Ag determination (titer larger than 1:8) either during the period of dialysis or within 2 months after transplantation. Of the children who became HB Ag positive during the period of dialysis, 89% demonstrated hepatitis B antigenemia during the initial 6 months of dialysis. Becuase of the temporal relationship between the development of HB Ag positivity and the duration of dialysis, contamination of equipment was proposed as a contributing source for transmission of HB Ag. Although 67% of the patients who were HB Ag positive during dialysis developed biochemical evidence of hepatic dysfunction, only two patients had clinical manifestations. The incidence of hepatic involvement appeared to be related to age; only one of nine children less than 12 years of age had evidence of hepatic disease. Intermittent persistence of HB Ag for periods larger than 3 years was observed. Both the incidence and persistence of HB Ag indicate the need to prevent its acquisition by pediatric patients undergoing hemodialysis.", "contents": "Hepatitis B in a pediatric hemodialysis unit. Serial HB Ag determinations were obtained on 62 children undergoing hemodialysis during a 31/2-year period. Thirty six (58%) of the patients had atleast one positive HB Ag determination (titer larger than 1:8) either during the period of dialysis or within 2 months after transplantation. Of the children who became HB Ag positive during the period of dialysis, 89% demonstrated hepatitis B antigenemia during the initial 6 months of dialysis. Becuase of the temporal relationship between the development of HB Ag positivity and the duration of dialysis, contamination of equipment was proposed as a contributing source for transmission of HB Ag. Although 67% of the patients who were HB Ag positive during dialysis developed biochemical evidence of hepatic dysfunction, only two patients had clinical manifestations. The incidence of hepatic involvement appeared to be related to age; only one of nine children less than 12 years of age had evidence of hepatic disease. Intermittent persistence of HB Ag for periods larger than 3 years was observed. Both the incidence and persistence of HB Ag indicate the need to prevent its acquisition by pediatric patients undergoing hemodialysis."}
{"id": "PMID:1089776", "title": "Hypertension after renal transplantation in children.", "content": "Hypertension persisted for longer than 6 mo or developed de novo after the first month following transplantation in seven of 77 pediatric recipients of renal allografts; concomitantly there were an elevation of PRA and renal angiographic abnormalities. In two of the four patients who developed RAS there was evidence of diminished allograft function. Successful correction of the stenotic lesion in these two recipients resulted in a return of the blood pressure, PRA, and biochemical function of the allograft to normal. Unsuccessful attempts at surgical repair led to loss of the allograft in the other two patients with RAS. Intrarenal vascular and/or parenchymal lesions were evident in the other three recipients with hypertension. Although an explanation was not apparent, subclinical rejection was hypothesized. Treatment effected reduction of the hypertension in these three patients and no deterioration of allograft function was observed for periods of 5, 34, and 38 mo, respectively. Renal angiographic studies and determinations of PRA are recommended in any pediatric recipient of an allograft who develops hypertension after the first month following transplantation or has hypertension which persists for longer than 6 mo after transplantation.", "contents": "Hypertension after renal transplantation in children. Hypertension persisted for longer than 6 mo or developed de novo after the first month following transplantation in seven of 77 pediatric recipients of renal allografts; concomitantly there were an elevation of PRA and renal angiographic abnormalities. In two of the four patients who developed RAS there was evidence of diminished allograft function. Successful correction of the stenotic lesion in these two recipients resulted in a return of the blood pressure, PRA, and biochemical function of the allograft to normal. Unsuccessful attempts at surgical repair led to loss of the allograft in the other two patients with RAS. Intrarenal vascular and/or parenchymal lesions were evident in the other three recipients with hypertension. Although an explanation was not apparent, subclinical rejection was hypothesized. Treatment effected reduction of the hypertension in these three patients and no deterioration of allograft function was observed for periods of 5, 34, and 38 mo, respectively. Renal angiographic studies and determinations of PRA are recommended in any pediatric recipient of an allograft who develops hypertension after the first month following transplantation or has hypertension which persists for longer than 6 mo after transplantation."}
{"id": "PMID:1089778", "title": "Comparison of House-Tree-Person drawings of young deaf and hearing children.", "content": "The House-Tree-Person (H-T-P) drawings of a matched sample of 80 deaf and 80 hearing school children, ages 7 through 10 years, were compared to assess differences related to the handicap, and the capacity of the H-T-P to distinguish between children rated by their teachers as poorly adjusted and those rated well adjusted. No differences were found between deaf and hearing in the drawing of the ear or mouth of the human figure. There was a very significant difference in the drawing of the branch structure of the tree. There were no differences in numbers of indicators of disturbance in the drawings of the children rated as more or as less well adjusted.", "contents": "Comparison of House-Tree-Person drawings of young deaf and hearing children. The House-Tree-Person (H-T-P) drawings of a matched sample of 80 deaf and 80 hearing school children, ages 7 through 10 years, were compared to assess differences related to the handicap, and the capacity of the H-T-P to distinguish between children rated by their teachers as poorly adjusted and those rated well adjusted. No differences were found between deaf and hearing in the drawing of the ear or mouth of the human figure. There was a very significant difference in the drawing of the branch structure of the tree. There were no differences in numbers of indicators of disturbance in the drawings of the children rated as more or as less well adjusted."}
{"id": "PMID:1089779", "title": "A scaled-projective measure of interpersonal values.", "content": "A projective instrument entitled the \"Kilmann Insight Test\" (KIT) was devised to measure the Interpersonal Value Constructs (IVCs) of individuals, defined as; the mental categories through which an individual perceives and interprets the desirable and undesirable features of interpersonal behavior. The KIT requires an individual to differentiate on a seven-point scal, 18 IVCs according to how relevant they are to a series of six ambiguous pictures of interpersonal situations. A factor analysis of the KIT's values resulted in two personal Restraint versus Boldness. Comparing the KIT with a self-report instrument containing the same value items as the KIT, seems to be tapping a different concept of values than the self-report assessment.", "contents": "A scaled-projective measure of interpersonal values. A projective instrument entitled the \"Kilmann Insight Test\" (KIT) was devised to measure the Interpersonal Value Constructs (IVCs) of individuals, defined as; the mental categories through which an individual perceives and interprets the desirable and undesirable features of interpersonal behavior. The KIT requires an individual to differentiate on a seven-point scal, 18 IVCs according to how relevant they are to a series of six ambiguous pictures of interpersonal situations. A factor analysis of the KIT's values resulted in two personal Restraint versus Boldness. Comparing the KIT with a self-report instrument containing the same value items as the KIT, seems to be tapping a different concept of values than the self-report assessment."}
{"id": "PMID:1089780", "title": "Hostility measurement and its relationship to fantasy capacity.", "content": "Two-hundred and three male high school freshmen and sophomore students were administered a daydreaming questionnaire, Buss-Durkee Hostility Inventory, and Rotter ISB. The latter was scored for hostility by the Renner, Maher, and Campbell method. The Subjects also rated one another on a sociometric scale measuring behavioral hostility. Subjects were divided into high and low fantasy groups. Analyses of variance were computed on the hostility measure data for 108 Subjects. The independent variables were obtained for the fantasy capacity measure and not for the peer rating measure. There was no positive correlation between the sociometric ratings and the hostility measures. In view of the results, the hostility measurement instruments are interpreted as measures of an individual's capacity to regard himself as hostile, rather than as measures of the actual hostility of his behavoir.", "contents": "Hostility measurement and its relationship to fantasy capacity. Two-hundred and three male high school freshmen and sophomore students were administered a daydreaming questionnaire, Buss-Durkee Hostility Inventory, and Rotter ISB. The latter was scored for hostility by the Renner, Maher, and Campbell method. The Subjects also rated one another on a sociometric scale measuring behavioral hostility. Subjects were divided into high and low fantasy groups. Analyses of variance were computed on the hostility measure data for 108 Subjects. The independent variables were obtained for the fantasy capacity measure and not for the peer rating measure. There was no positive correlation between the sociometric ratings and the hostility measures. In view of the results, the hostility measurement instruments are interpreted as measures of an individual's capacity to regard himself as hostile, rather than as measures of the actual hostility of his behavoir."}
{"id": "PMID:1089781", "title": "Studies of biologic parameters for denture design. Part I. Comparison of masseter muscle activity during chewing of crisp and soggy wafers in denture and dentition groups.", "content": "The motor response to two textures was studied by comparing the EMG activities of the ipsilateral muscle during 5 minute periods of eating crisp and soggy wafers in each group of subjects. In both groups, the activity of the ipsilateral muscle was significantly greater for eating crisp wafers than for eating soggy wafers. The ratio of EMG activities for crisp and soggy textures was 1.48 in the dentition group and 1.72 for the denture wearers. These differences between the activities of ipsilateral muscles for the two textures were significant in both groups (P smaller than 0.05). Thus, in terms of muscle effort, denture wearers display discrimination of textural characteristics of food during chewing similar to that shown by subjects with natural teeth.", "contents": "Studies of biologic parameters for denture design. Part I. Comparison of masseter muscle activity during chewing of crisp and soggy wafers in denture and dentition groups. The motor response to two textures was studied by comparing the EMG activities of the ipsilateral muscle during 5 minute periods of eating crisp and soggy wafers in each group of subjects. In both groups, the activity of the ipsilateral muscle was significantly greater for eating crisp wafers than for eating soggy wafers. The ratio of EMG activities for crisp and soggy textures was 1.48 in the dentition group and 1.72 for the denture wearers. These differences between the activities of ipsilateral muscles for the two textures were significant in both groups (P smaller than 0.05). Thus, in terms of muscle effort, denture wearers display discrimination of textural characteristics of food during chewing similar to that shown by subjects with natural teeth."}
{"id": "PMID:1089782", "title": "Selectively retained vital roots for partial support of overdentures: a patient report.", "content": "Vital mandibular canine roots were submerged submucosally ina 52-year-old man. The events that occurred during 27 months of observation, while the patient wore dentures over the retained roots, were described.", "contents": "Selectively retained vital roots for partial support of overdentures: a patient report. Vital mandibular canine roots were submerged submucosally ina 52-year-old man. The events that occurred during 27 months of observation, while the patient wore dentures over the retained roots, were described."}
{"id": "PMID:1089784", "title": "A ceramic restoration bonded by etched enamel and resin for fractured incisors.", "content": "This report describes a technique for making ceramic restorations for fractured incisors without operative interference. The ceramic block is baked in the laboratory on a 24 karat gold matrix cast. A resin is bonded to a silane-treated porcelain block and etched enamel.", "contents": "A ceramic restoration bonded by etched enamel and resin for fractured incisors. This report describes a technique for making ceramic restorations for fractured incisors without operative interference. The ceramic block is baked in the laboratory on a 24 karat gold matrix cast. A resin is bonded to a silane-treated porcelain block and etched enamel."}
{"id": "PMID:1089792", "title": "Malignant lymphomas of follicular center cell origin in man. I. Immunologic studies.", "content": "Lymphomas with histologic features indicating a follicular center cell (FCC) origin were analyzed from 26 patients of a group of 45 consecutive non-Hodgkin's lymphoma patinets whose tumors were studied for B- and T-cell characteristics. They were compared with benign, reactive lymphoid tissue from 14 patients. Cell suspensions from biopsy material, blood, or bone marrow were examined for surface Ig and for rosette formation with sheep erythrocytes (E rosettes). Of the 26 patients with FCC lymphomas, 22 had 40% or more Ig-bearing cells; all patients with FCC lymphoma tissues had 25% or less E rosette-forming cells. Cells from most FCC lymphomas of the cleaved type had surfac IgM; those from several FCC lymphomas had both IgM and IgD. Cells from lymphomas of noncleaved cell type had surface IgG or IgA. Light-chain analysis showed that cells from FCC lymphomas bore a predominant light-chain type, which indicated their monoclonal nature. Neoplastic cells from several FCC lymphomas synthesized the surface Ig which they bore. Reactive tissues usually contained fewer Ig-bearing and more E rosette-forming cells than FCC lymphomas; the Ig-bearing cells, with one exception, had a polyclonal distribution. Correlation of histologic and immunologic observations indicates that most lymphomas identified as FCC in origin by light micorscopic criteria mark as B cells with the use of immunologic techniques and that FCC lymphomas are the most common type of non-Hodgkin's lymphoma.", "contents": "Malignant lymphomas of follicular center cell origin in man. I. Immunologic studies. Lymphomas with histologic features indicating a follicular center cell (FCC) origin were analyzed from 26 patients of a group of 45 consecutive non-Hodgkin's lymphoma patinets whose tumors were studied for B- and T-cell characteristics. They were compared with benign, reactive lymphoid tissue from 14 patients. Cell suspensions from biopsy material, blood, or bone marrow were examined for surface Ig and for rosette formation with sheep erythrocytes (E rosettes). Of the 26 patients with FCC lymphomas, 22 had 40% or more Ig-bearing cells; all patients with FCC lymphoma tissues had 25% or less E rosette-forming cells. Cells from most FCC lymphomas of the cleaved type had surfac IgM; those from several FCC lymphomas had both IgM and IgD. Cells from lymphomas of noncleaved cell type had surface IgG or IgA. Light-chain analysis showed that cells from FCC lymphomas bore a predominant light-chain type, which indicated their monoclonal nature. Neoplastic cells from several FCC lymphomas synthesized the surface Ig which they bore. Reactive tissues usually contained fewer Ig-bearing and more E rosette-forming cells than FCC lymphomas; the Ig-bearing cells, with one exception, had a polyclonal distribution. Correlation of histologic and immunologic observations indicates that most lymphomas identified as FCC in origin by light micorscopic criteria mark as B cells with the use of immunologic techniques and that FCC lymphomas are the most common type of non-Hodgkin's lymphoma."}
{"id": "PMID:1089793", "title": "Immune complexes in cancer: demonstration of complexes in mice bearing neuroblastomas.", "content": "Immune complexes occurred in a large proportion of mice with syngeneic neuroblastomas. Circulating immune complexes formed in all mice, whereas almost 50% had deposits of host IgG and complement in a granular pattern along the mesangia and basement membranes of the renal glomeruli. The bound IgG was eluted and recovered from the glomeruli and demonstrated immunologic specificity to neuroblastoma cell-surface antigens.", "contents": "Immune complexes in cancer: demonstration of complexes in mice bearing neuroblastomas. Immune complexes occurred in a large proportion of mice with syngeneic neuroblastomas. Circulating immune complexes formed in all mice, whereas almost 50% had deposits of host IgG and complement in a granular pattern along the mesangia and basement membranes of the renal glomeruli. The bound IgG was eluted and recovered from the glomeruli and demonstrated immunologic specificity to neuroblastoma cell-surface antigens."}
{"id": "PMID:1089794", "title": "Malignant lymphomas of follicular center cell origin in man. II. Ultrastructural and cytochemical studies.", "content": "Tissues from malignant lymphomas with both nodular and diffuse growth patterns, thought by light microscopy to be composed of cells of follicular center cell (FCC) origin, Were examined by electron microscopy; the tumor cells were similar to lymphoid cells found in reactive follicular centers. Tumor cells from neoplasms thought to be composed of cleaved FCC often had more pronounced nuclear folding than did cleaved FCC of reactive follicles, whereas cells in tumors of noncleaved FCC type were indistinguishable from their presumed counterparts in reactive follicles. Large cell noeplasms, previously classified as \"histiocytic\" lymphomas were composed of cells with ultrastructural characteristics of transformed lymphocytes; they showed neither ultrastructural nor cytochemical features of mononuclear phagocytes. These findings support the concept that a major group of lymphomas arises from lymphocytes of follicular centers.", "contents": "Malignant lymphomas of follicular center cell origin in man. II. Ultrastructural and cytochemical studies. Tissues from malignant lymphomas with both nodular and diffuse growth patterns, thought by light microscopy to be composed of cells of follicular center cell (FCC) origin, Were examined by electron microscopy; the tumor cells were similar to lymphoid cells found in reactive follicular centers. Tumor cells from neoplasms thought to be composed of cleaved FCC often had more pronounced nuclear folding than did cleaved FCC of reactive follicles, whereas cells in tumors of noncleaved FCC type were indistinguishable from their presumed counterparts in reactive follicles. Large cell noeplasms, previously classified as \"histiocytic\" lymphomas were composed of cells with ultrastructural characteristics of transformed lymphocytes; they showed neither ultrastructural nor cytochemical features of mononuclear phagocytes. These findings support the concept that a major group of lymphomas arises from lymphocytes of follicular centers."}
{"id": "PMID:1089795", "title": "Detection of T-cell surface antigens in a Marek's disease lymphoblastoid cell line.", "content": "Indirect membrane immunofluorescence was applied in the study of a lymphoblastoid cell line derived from Marek's disease lymphoma. The cell line tacked surface immunoglobulins and reacted specifically with antisera prepared against chicken thymus cells. Cells transformed by Marek's disease virus were of thymus origin.", "contents": "Detection of T-cell surface antigens in a Marek's disease lymphoblastoid cell line. Indirect membrane immunofluorescence was applied in the study of a lymphoblastoid cell line derived from Marek's disease lymphoma. The cell line tacked surface immunoglobulins and reacted specifically with antisera prepared against chicken thymus cells. Cells transformed by Marek's disease virus were of thymus origin."}
{"id": "PMID:1089796", "title": "Enhancement and retardation of spontaneous reticulum cell neoplasm development in SJL/J mice.", "content": "The enhancement or delay in the appearance of spontaneous reticulum cell neoplasms (RCN-B) in SJL/J mice was tested. Immunosuppressive treatment with antithymocyte serum and prolonged antigenic stimulation by repeated injections of sheep erythrocytes, leukemogenic cell-free centrifugates, and multichain synthetic polypeptides had no effect on the incidence or latency of spontaneous tumor appearance. Treatment with mineral oil or incomplete Freund adjuvant markedly enhanced tumor appearance (though the abundance of plasma cells induced by these treatments did not evoke the development of plasmacytomas, nor did it affect the incidence of serum paraproteinemia among mice with spontaneous tumors). Treatment with cortisone acetate, however, markedly retarded spontaneous tumor development. Tumors occurring early due to mineral oil and adjuvant treatment all had the characteristics of \"RCN-B plasma cell type,\" and tumors occurring late due to cortisone treatment were all of the \"RCN-B reticulum cell type.\" The possible involvement of the stem cell pool size in the enhancement or delay of spontaneous tumor development was discussed.", "contents": "Enhancement and retardation of spontaneous reticulum cell neoplasm development in SJL/J mice. The enhancement or delay in the appearance of spontaneous reticulum cell neoplasms (RCN-B) in SJL/J mice was tested. Immunosuppressive treatment with antithymocyte serum and prolonged antigenic stimulation by repeated injections of sheep erythrocytes, leukemogenic cell-free centrifugates, and multichain synthetic polypeptides had no effect on the incidence or latency of spontaneous tumor appearance. Treatment with mineral oil or incomplete Freund adjuvant markedly enhanced tumor appearance (though the abundance of plasma cells induced by these treatments did not evoke the development of plasmacytomas, nor did it affect the incidence of serum paraproteinemia among mice with spontaneous tumors). Treatment with cortisone acetate, however, markedly retarded spontaneous tumor development. Tumors occurring early due to mineral oil and adjuvant treatment all had the characteristics of \"RCN-B plasma cell type,\" and tumors occurring late due to cortisone treatment were all of the \"RCN-B reticulum cell type.\" The possible involvement of the stem cell pool size in the enhancement or delay of spontaneous tumor development was discussed."}
{"id": "PMID:1089800", "title": "Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells.", "content": "The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a hybridization assay, has indicated the presence in infected cells of 18 distinct RNA species with sizes up to and greater than the unit (viral) length. The sizes of phiX mRNA's were similar irrespective of whether material was analyzed on gels or in dimethyl sulfoxide gradients. When virus-induced RNA was detected by a double-label method, seven additional low-molecular weight species were observed on gels and the resolution of dimethyl sulfoxide gradients was enhanced. The present results lend support to aspects of the model of Hayashi and Hayashi (1970) for the generation of these discrete mRNA species; an alternative model is also discussed.", "contents": "Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells. The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a hybridization assay, has indicated the presence in infected cells of 18 distinct RNA species with sizes up to and greater than the unit (viral) length. The sizes of phiX mRNA's were similar irrespective of whether material was analyzed on gels or in dimethyl sulfoxide gradients. When virus-induced RNA was detected by a double-label method, seven additional low-molecular weight species were observed on gels and the resolution of dimethyl sulfoxide gradients was enhanced. The present results lend support to aspects of the model of Hayashi and Hayashi (1970) for the generation of these discrete mRNA species; an alternative model is also discussed."}
{"id": "PMID:1089801", "title": "Mutations in the new gene stIII of bacteriophage T4B suppressing the lysis defect of gene stII and a gene e mutant.", "content": "Mutations of bacteriophage T4B were found which suppress the lysis defect of both gene stII mutants and gene e mutants. The suppressor mutations belong to a new gene, stIII, of phage T4B. Gene stIII is located on the genetic map of T4B between genes stI and e. stIII mutants sometimes form star plaques on Escherichia coli B. The latent period on E. coli 594, but not E. coli B, is shorter with stIII mutants than that with wild-type phage. The premature lysis of E. coli 594 infected with stIII phage does not depend on the expression of both stII+ and e+ function. StIII allele is dominant over the stIII+ with respect to both the ability to suppress the stII defect and the early lysis of infected E. coli 594 cultures.", "contents": "Mutations in the new gene stIII of bacteriophage T4B suppressing the lysis defect of gene stII and a gene e mutant. Mutations of bacteriophage T4B were found which suppress the lysis defect of both gene stII mutants and gene e mutants. The suppressor mutations belong to a new gene, stIII, of phage T4B. Gene stIII is located on the genetic map of T4B between genes stI and e. stIII mutants sometimes form star plaques on Escherichia coli B. The latent period on E. coli 594, but not E. coli B, is shorter with stIII mutants than that with wild-type phage. The premature lysis of E. coli 594 infected with stIII phage does not depend on the expression of both stII+ and e+ function. StIII allele is dominant over the stIII+ with respect to both the ability to suppress the stII defect and the early lysis of infected E. coli 594 cultures."}
{"id": "PMID:1089802", "title": "Host DNA degradation after infection of Escherichia coli with bacteriophage T4: dependence of the alternate pathway of degradation which occurs in the absence of both T4 endonuclease II and nuclear disruption on T4 endonuclease IV.", "content": "Escherichia coli cells infected with T4 phage which are deficient in both nuclear disruption and endonuclease II exhibit a pathway of host DNA degradation which does not occur in cells infected with phage deficient only in endonuclease II. This alternate pathway of host DNA degradation requires T4 endonuclease IV.", "contents": "Host DNA degradation after infection of Escherichia coli with bacteriophage T4: dependence of the alternate pathway of degradation which occurs in the absence of both T4 endonuclease II and nuclear disruption on T4 endonuclease IV. Escherichia coli cells infected with T4 phage which are deficient in both nuclear disruption and endonuclease II exhibit a pathway of host DNA degradation which does not occur in cells infected with phage deficient only in endonuclease II. This alternate pathway of host DNA degradation requires T4 endonuclease IV."}
{"id": "PMID:1089803", "title": "Canavanine-mediated depletion of polyamine pools in Escherichia coli: effect of head morphogenesis and DNA synthesis.", "content": "We have found that L-canavanine inhibited the synthesis of polyamines in T4-infected Escherichia coli. These polyamines are known to be required for T4 DNA synthesis and may be involved in phage morphogenesis. The new data indicate that the inhibition of polyamine synthesis is not primarily responsible for the L-conavanine-mediated inhibition of DNA synthesis nor does it seem to be involved in the induction of lollipops. L-Canavanine does influence the relative amounts of putrescine and spermidine found in the phage particle, but it does not influence the amount of DNA phosphate neutralized by polyamines.", "contents": "Canavanine-mediated depletion of polyamine pools in Escherichia coli: effect of head morphogenesis and DNA synthesis. We have found that L-canavanine inhibited the synthesis of polyamines in T4-infected Escherichia coli. These polyamines are known to be required for T4 DNA synthesis and may be involved in phage morphogenesis. The new data indicate that the inhibition of polyamine synthesis is not primarily responsible for the L-conavanine-mediated inhibition of DNA synthesis nor does it seem to be involved in the induction of lollipops. L-Canavanine does influence the relative amounts of putrescine and spermidine found in the phage particle, but it does not influence the amount of DNA phosphate neutralized by polyamines."}
{"id": "PMID:1089804", "title": "Growth of a capsid mutant of bacteriophage phi X174 in a temperature-sensitive strain of Escherichia coli.", "content": "A capsid mutant of phiX174 is capable of forming replicative form and synthesizing single strands at the restrictive temperature in a dnaB mutant of Escherichia coli. Under similar conditions, the wild-type bacteriophage is incapable of either step in viral synthesis.", "contents": "Growth of a capsid mutant of bacteriophage phi X174 in a temperature-sensitive strain of Escherichia coli. A capsid mutant of phiX174 is capable of forming replicative form and synthesizing single strands at the restrictive temperature in a dnaB mutant of Escherichia coli. Under similar conditions, the wild-type bacteriophage is incapable of either step in viral synthesis."}
{"id": "PMID:1089805", "title": "Biological activity of T4 DNA synthesized in toluene-treated Escherichia coli cells.", "content": "T4 DNA synthesized in a toluene-treated cell system can act as the genetic donor in a DNA transformation assay. This material transforms a variety of markers at high efficiency. We present evidence that the genetic activity is due to newly synthesized, double-stranded DNA.", "contents": "Biological activity of T4 DNA synthesized in toluene-treated Escherichia coli cells. T4 DNA synthesized in a toluene-treated cell system can act as the genetic donor in a DNA transformation assay. This material transforms a variety of markers at high efficiency. We present evidence that the genetic activity is due to newly synthesized, double-stranded DNA."}
{"id": "PMID:1089806", "title": "In Vitro system for the study of bacteriophage phi chi 174 adsorption and eclipse.", "content": "An in vitro system was developed for the study of the initial stages of bacteriophage phi chi 174 infection. Escherichia coli C cells were incubated with 20% sucrose and then subjected to cold osmotic shock in 5 mM MgSO4. The concentrated supernatant shock fluid inactivated phi chi 174 with the same kinetics and requirements as for normal infection. Shock fluids prepared from phi chi 174-resistant strains of E. coli did not show this effect. The 114S phage were initially converted into 70S particles, the process termed \"eclipse\". These structurally altered phages then attached to a component of the shock fluid, producing fast-sedimenting complexes, and eventually released at least a part of their DNA into the medium. The fast-sedimenting complex could be radioactively labeled with oleic acid. Radioactivity was found to co-chromatograph with both biological activity and the majority of the high-molecular-weight carbohydrates present in the shock fluid. It is concluded that E. coli C osmotic shock fluid contains isolated phi chi 174-specific receptor sites composed of lipopolysaccharides. This system conveniently separates the early stages of phage phi chi 174 infection from the intracellular events.", "contents": "In Vitro system for the study of bacteriophage phi chi 174 adsorption and eclipse. An in vitro system was developed for the study of the initial stages of bacteriophage phi chi 174 infection. Escherichia coli C cells were incubated with 20% sucrose and then subjected to cold osmotic shock in 5 mM MgSO4. The concentrated supernatant shock fluid inactivated phi chi 174 with the same kinetics and requirements as for normal infection. Shock fluids prepared from phi chi 174-resistant strains of E. coli did not show this effect. The 114S phage were initially converted into 70S particles, the process termed \"eclipse\". These structurally altered phages then attached to a component of the shock fluid, producing fast-sedimenting complexes, and eventually released at least a part of their DNA into the medium. The fast-sedimenting complex could be radioactively labeled with oleic acid. Radioactivity was found to co-chromatograph with both biological activity and the majority of the high-molecular-weight carbohydrates present in the shock fluid. It is concluded that E. coli C osmotic shock fluid contains isolated phi chi 174-specific receptor sites composed of lipopolysaccharides. This system conveniently separates the early stages of phage phi chi 174 infection from the intracellular events."}
{"id": "PMID:1089808", "title": "Technique of reductive ureteroplasty in the management of megaureter.", "content": "Management of megaureter may be conservative or surgical. Most operative procedures are multistaged and directed toward segmental ureteral repair. We have treated succesfully a number of patients with the total linear ureteral reduction technique.", "contents": "Technique of reductive ureteroplasty in the management of megaureter. Management of megaureter may be conservative or surgical. Most operative procedures are multistaged and directed toward segmental ureteral repair. We have treated succesfully a number of patients with the total linear ureteral reduction technique."}
{"id": "PMID:1089807", "title": "Identification and characterization of the in vitro synthesized gene products of bacteriophage M13.", "content": "Bacteriophage M13 replicative form (RF) DNA was used to direct coupled transcription and translation in cell-free extracts prepared from Escherichia coli. By using RF DNA, isolated from cells infected with appropriate amber mutants of this phage, it has been possible to identify the products of genes I through IV. By using the same methods no gene-product relationship could be demonstrated for genes VI and VII. Coupled in vitro protein synthesis studies on RF-III DNA, a linear double-stranded DNA molecule, obtained after cleavage of either RF-I or RF-II DNA with the restriction endonuclease R.Hin11 from Haemophilus influenzae, indicated that the cleavage site for this enzyme is located in gene II. The in vitro products of both gene III and gene VIII are about 30 and six amino acids longer, respectively, than their native counterparts present within the virion. These results suggest that the latter proteins arise in vivo by cleavage of precursor molecules. Coupled transcription and translation studies on a DNA fragment which only contained the genetic information coding for gene IV protein, obtained after cleavage of RF DNA with the restriction endonuclease R.Hap11 from Haemophilus aphirophilus, indicated that a large number of the in vitro synthesized polypeptides are the result of premature chain termination.", "contents": "Identification and characterization of the in vitro synthesized gene products of bacteriophage M13. Bacteriophage M13 replicative form (RF) DNA was used to direct coupled transcription and translation in cell-free extracts prepared from Escherichia coli. By using RF DNA, isolated from cells infected with appropriate amber mutants of this phage, it has been possible to identify the products of genes I through IV. By using the same methods no gene-product relationship could be demonstrated for genes VI and VII. Coupled in vitro protein synthesis studies on RF-III DNA, a linear double-stranded DNA molecule, obtained after cleavage of either RF-I or RF-II DNA with the restriction endonuclease R.Hin11 from Haemophilus influenzae, indicated that the cleavage site for this enzyme is located in gene II. The in vitro products of both gene III and gene VIII are about 30 and six amino acids longer, respectively, than their native counterparts present within the virion. These results suggest that the latter proteins arise in vivo by cleavage of precursor molecules. Coupled transcription and translation studies on a DNA fragment which only contained the genetic information coding for gene IV protein, obtained after cleavage of RF DNA with the restriction endonuclease R.Hap11 from Haemophilus aphirophilus, indicated that a large number of the in vitro synthesized polypeptides are the result of premature chain termination."}
{"id": "PMID:1089809", "title": "Bench surgery for tumor in a solitary kidney.", "content": "Bench surgery which included ex vivo partial nephrectomy with subsequent autotransplantation was successfully performed on a solitary kidney containing renal cell carcinoma. Helpful adjuncts included perfusion with a hyperosmolar intracellular-like solution, perfusion with a preservation machine and upside-down ipsilateral transplantation.", "contents": "Bench surgery for tumor in a solitary kidney. Bench surgery which included ex vivo partial nephrectomy with subsequent autotransplantation was successfully performed on a solitary kidney containing renal cell carcinoma. Helpful adjuncts included perfusion with a hyperosmolar intracellular-like solution, perfusion with a preservation machine and upside-down ipsilateral transplantation."}
{"id": "PMID:1089813", "title": "Dorsal rectotomy and full thickness skin graft for repair of prostatic urethrorectal fistula.", "content": "Management of a case of postoperative prostatic urethrorectal fistula is presented wherein the standard perineal repair failed. Successful repair was achieved by using a full thickness penile skin patch graft placed through a dorsal rectotomy incision.", "contents": "Dorsal rectotomy and full thickness skin graft for repair of prostatic urethrorectal fistula. Management of a case of postoperative prostatic urethrorectal fistula is presented wherein the standard perineal repair failed. Successful repair was achieved by using a full thickness penile skin patch graft placed through a dorsal rectotomy incision."}
{"id": "PMID:1089814", "title": "A safe, simple and reliable method for percutaneous (closed) renal biopsies in children: results in 100 consecutive patients.", "content": "A safe, simple and reliable technique for percutaneous renal biopsy in children has been described. Specimens adequate for pathologic interpretation were obtained in 97 patients. Significant complications (gross hematuria lasting more than 36 hours, severe pain, perirenal mass or unexplained fever) occurred in 8 patients. No secondary operations or transfusions were required. No mortailty or late complications occurred.", "contents": "A safe, simple and reliable method for percutaneous (closed) renal biopsies in children: results in 100 consecutive patients. A safe, simple and reliable technique for percutaneous renal biopsy in children has been described. Specimens adequate for pathologic interpretation were obtained in 97 patients. Significant complications (gross hematuria lasting more than 36 hours, severe pain, perirenal mass or unexplained fever) occurred in 8 patients. No secondary operations or transfusions were required. No mortailty or late complications occurred."}
{"id": "PMID:1089815", "title": "Renal transplantation in pediatric patients.", "content": "Thirty-five transplants have been performed in 29 children, 1 week to 16 years old. Of these patients 79 per cent are surviving from 9 months to 14 years post-transplantation. Eighteen of these patients have required different surgical procedures for transplantation than adult patients. Immunosuppressive therapy has been essentially the same as in adult patients. A striking difference between the living related donor and the cadaver donor transplant functional survival as seen in this series is unexplained at the present time. Linear growth has been good in those children who have required minimal doses of corticosteroids to maintain adequate renal function.", "contents": "Renal transplantation in pediatric patients. Thirty-five transplants have been performed in 29 children, 1 week to 16 years old. Of these patients 79 per cent are surviving from 9 months to 14 years post-transplantation. Eighteen of these patients have required different surgical procedures for transplantation than adult patients. Immunosuppressive therapy has been essentially the same as in adult patients. A striking difference between the living related donor and the cadaver donor transplant functional survival as seen in this series is unexplained at the present time. Linear growth has been good in those children who have required minimal doses of corticosteroids to maintain adequate renal function."}
{"id": "PMID:1089816", "title": "Cryptococcus neoformans as a cause of lytic bone lesions.", "content": "Cryptococcosis is a disseminated infection of man and animals that involves many organs, especially the central nervous system. Isolated bone involvement may cause lytic bone lesions. Diagnosis may be made by pathologic sections and culture of specimens obtained through surgical biopsy. When Cryptococcus is recovered from bone, care must be taken to study other organ systems, especially the central nervous system. Isolated bone disease without meningitis is very uncommom. Intense medical therapy is necessary for treatment of meningitis; isolated osteomyelitis may respond to surgical drainage alone.", "contents": "Cryptococcus neoformans as a cause of lytic bone lesions. Cryptococcosis is a disseminated infection of man and animals that involves many organs, especially the central nervous system. Isolated bone involvement may cause lytic bone lesions. Diagnosis may be made by pathologic sections and culture of specimens obtained through surgical biopsy. When Cryptococcus is recovered from bone, care must be taken to study other organ systems, especially the central nervous system. Isolated bone disease without meningitis is very uncommom. Intense medical therapy is necessary for treatment of meningitis; isolated osteomyelitis may respond to surgical drainage alone."}
{"id": "PMID:1089817", "title": "Ascorbic acid and the common cold. Evaluation of its efficacy and toxicity.", "content": "We reviewed the clinical data relating to the efficacy and safety of pharmacologic doses of ascorbic acid in the prevention and treatment of the common cold. Although one study tentatively supports the hypothesis that such doses of ascorbic acid may be efficacious, a second study by the same group did not confirm the significant findings, and no clear, reproducible pattern of efficacy has emerged from the review of all the evidence. Similarly, there is currently little adequate evidence on either the presence or the absence of serious adverse reactions to such doses of ascorbic acid, although many such reactions have been hypothesized. The unrestricted use of ascorbic acid for these purposes cannot be advocated on the basis of the evidence currently available.", "contents": "Ascorbic acid and the common cold. Evaluation of its efficacy and toxicity. We reviewed the clinical data relating to the efficacy and safety of pharmacologic doses of ascorbic acid in the prevention and treatment of the common cold. Although one study tentatively supports the hypothesis that such doses of ascorbic acid may be efficacious, a second study by the same group did not confirm the significant findings, and no clear, reproducible pattern of efficacy has emerged from the review of all the evidence. Similarly, there is currently little adequate evidence on either the presence or the absence of serious adverse reactions to such doses of ascorbic acid, although many such reactions have been hypothesized. The unrestricted use of ascorbic acid for these purposes cannot be advocated on the basis of the evidence currently available."}
{"id": "PMID:1089819", "title": "Antimicrobial resistance of Shigellae isolated in Michigan.", "content": "Patterns of antimicrobial resistance were studied for 213 strains of shigellae isolated in rural and urban areas of Michigan by quantitative techniques: 66% of these organisms were resistant to ampicillin, tetracycline, cephalexin, or chloramphenicol or had multiple resistance to various combinations of these antibiotics. The incidence of resistant organisms was higher in the Detroit-Wayne County area, which is the most urban part of the state. Nalidixic acid and oxolinic acid were noted to be quite effective in vitro against shigellae and perhaps warrant further clinical study as potential agents for therapy of shigellosis, especially in view of the appearance of isolates with multiple resistance. Sulfamethoxazole alone was effective against only 62% of the Shigella isolates tested, but mixture with trimethoprim in a 20:1 ratio yielded a highly active in vitro combination.", "contents": "Antimicrobial resistance of Shigellae isolated in Michigan. Patterns of antimicrobial resistance were studied for 213 strains of shigellae isolated in rural and urban areas of Michigan by quantitative techniques: 66% of these organisms were resistant to ampicillin, tetracycline, cephalexin, or chloramphenicol or had multiple resistance to various combinations of these antibiotics. The incidence of resistant organisms was higher in the Detroit-Wayne County area, which is the most urban part of the state. Nalidixic acid and oxolinic acid were noted to be quite effective in vitro against shigellae and perhaps warrant further clinical study as potential agents for therapy of shigellosis, especially in view of the appearance of isolates with multiple resistance. Sulfamethoxazole alone was effective against only 62% of the Shigella isolates tested, but mixture with trimethoprim in a 20:1 ratio yielded a highly active in vitro combination."}
{"id": "PMID:1089828", "title": "A method for quantitative detection of tricuspid regurgitation with double injection--single sampling dye-dilution technique.", "content": "A method for quantitative detection of tricuspid regurgitation is proposed. In cases without tricuspid insufficiency or intracardiac shunt, 1/slope (ts-fv) of the earpiece dye-dilution curve after injection of dye into the femoreal vein (fv curve) was significantly correlated with that (ts-pa) of the curve after injection into the pulmonary artery (PA curve) within each range of right heart blood volume (rbv) calculated as a product of cardiac output and mean transit time difference between FV curve and PA curve. The relationship among the 3 parameters was expressed as a statistically significant regression equation; log (tsfv)  equals 0.9858 log (ts-pa) plus 0.2980 log (RBV) minus 0.6418 (p small than 0.005, sd of predicted ts-fv equals plus or minus 0.591). The assumption was made that tricuspid regurgitation caused prolongation ot ts-fv without any changes in ts-pa or RBV, being based on the concepts of the indicator dispersion and of the path-length distribution. The ratio of tricuspid regurgitation to cardiac output was calculated from (observed ts-fv-predicted ts-fv)/predicted ts-fv, in which predicted ts-fv is the value calculated from ts-pa and RBV using the equation shown above. In 2 patients who manifested clinical tricuspid insufficiency, large amount of tricuspid regurgitant fraction was determined by this method. It is suggested that the present method utilizing double injections--single sampling dye-dilution technique is useful for clinical quantitation of tricuspid regurgitation.", "contents": "A method for quantitative detection of tricuspid regurgitation with double injection--single sampling dye-dilution technique. A method for quantitative detection of tricuspid regurgitation is proposed. In cases without tricuspid insufficiency or intracardiac shunt, 1/slope (ts-fv) of the earpiece dye-dilution curve after injection of dye into the femoreal vein (fv curve) was significantly correlated with that (ts-pa) of the curve after injection into the pulmonary artery (PA curve) within each range of right heart blood volume (rbv) calculated as a product of cardiac output and mean transit time difference between FV curve and PA curve. The relationship among the 3 parameters was expressed as a statistically significant regression equation; log (tsfv)  equals 0.9858 log (ts-pa) plus 0.2980 log (RBV) minus 0.6418 (p small than 0.005, sd of predicted ts-fv equals plus or minus 0.591). The assumption was made that tricuspid regurgitation caused prolongation ot ts-fv without any changes in ts-pa or RBV, being based on the concepts of the indicator dispersion and of the path-length distribution. The ratio of tricuspid regurgitation to cardiac output was calculated from (observed ts-fv-predicted ts-fv)/predicted ts-fv, in which predicted ts-fv is the value calculated from ts-pa and RBV using the equation shown above. In 2 patients who manifested clinical tricuspid insufficiency, large amount of tricuspid regurgitant fraction was determined by this method. It is suggested that the present method utilizing double injections--single sampling dye-dilution technique is useful for clinical quantitation of tricuspid regurgitation."}
{"id": "PMID:1089835", "title": "Pattern of core and surface expression in liver tissue reflects state of specific immune response in hepatitis B.", "content": "A series of 67 liver biopsies (20 kidney transplant recipients and 47 outpatients with hepatitis) was investigated for the presence of hepatitis B antigen core (HBc) and surface (HBs) components by immunofluorescence and electron microscopy. The variable appearance of the core in liver cell nuclei and of the surface in the cytoplasm allowed the recognition of expression patterns which, together with histologic parameters, could be integrated into four reaction types of diagnostic and prognostic implications: Type I (Elimination Type). No components or only occasional expression of HBc; histologically, classic lobular hepatitis; clinically, acute, self-limited viral hepatitis. Type II (HBc Predominance, or Immunosuppression Type). Abundant core expression in each liver cell nucleus and moderate appearance of HBs; histologically, nonaggressive inflammation (nonspecific reactive or portal hepatitis); clinically, mild, chronic, persistent hepatitis in transplant patients. Type III (HBs Predominance, or Nonaggressive Type). Prominent HBs expression largely in the absence of HBc; histologically, nonaggressive inflammation (nonspecific reactive and portal hepatitis) or normal liver tissue, together with ground-glass hepatocytes in light microscopy, as a correlate of HBs-containing hepatocytes; clinically, hepatitis B antigen carrier, or chronic persistent hepatitis. Type IV (HBc+s Equivalence, or Aggressive Type). Spotty expression of both components, especially of core; histologically, periportal hepatitis; clinically, mainly corresponds to chronic aggressive hepatitis and to acute hepatitis with possible transition to chronicity. As a unifying concept for these types, it is suggested that immune responsiveness determines the reaction pattern, the key mechanism being immune elimination of affected cells. Between efficient elimination (type I) and effective immunosuppression (type II), a graded elimination insufficiency is found in chronic forms (types III and IV), explaining the persistence and probably also the aggressiveness of hepatitis B virus infection.", "contents": "Pattern of core and surface expression in liver tissue reflects state of specific immune response in hepatitis B. A series of 67 liver biopsies (20 kidney transplant recipients and 47 outpatients with hepatitis) was investigated for the presence of hepatitis B antigen core (HBc) and surface (HBs) components by immunofluorescence and electron microscopy. The variable appearance of the core in liver cell nuclei and of the surface in the cytoplasm allowed the recognition of expression patterns which, together with histologic parameters, could be integrated into four reaction types of diagnostic and prognostic implications: Type I (Elimination Type). No components or only occasional expression of HBc; histologically, classic lobular hepatitis; clinically, acute, self-limited viral hepatitis. Type II (HBc Predominance, or Immunosuppression Type). Abundant core expression in each liver cell nucleus and moderate appearance of HBs; histologically, nonaggressive inflammation (nonspecific reactive or portal hepatitis); clinically, mild, chronic, persistent hepatitis in transplant patients. Type III (HBs Predominance, or Nonaggressive Type). Prominent HBs expression largely in the absence of HBc; histologically, nonaggressive inflammation (nonspecific reactive and portal hepatitis) or normal liver tissue, together with ground-glass hepatocytes in light microscopy, as a correlate of HBs-containing hepatocytes; clinically, hepatitis B antigen carrier, or chronic persistent hepatitis. Type IV (HBc+s Equivalence, or Aggressive Type). Spotty expression of both components, especially of core; histologically, periportal hepatitis; clinically, mainly corresponds to chronic aggressive hepatitis and to acute hepatitis with possible transition to chronicity. As a unifying concept for these types, it is suggested that immune responsiveness determines the reaction pattern, the key mechanism being immune elimination of affected cells. Between efficient elimination (type I) and effective immunosuppression (type II), a graded elimination insufficiency is found in chronic forms (types III and IV), explaining the persistence and probably also the aggressiveness of hepatitis B virus infection."}
{"id": "PMID:1089836", "title": "Exocrine pancreatic dysfunction in guinea pigs with diabetes mellitus.", "content": "In this study, 37 guinea pigs were classified, on the basis of histologic examination of the pancreas, into three categories: nondiabetic, latent diabetic, and overt diabetic. In order to compare the exocrine pancreatic function in these three groups of animals, pancreatic secretion was collected from each animal following an intravenous infusion of secretin and pancreozymin. Pancreatic enzyme activity, bicarbonate concentration, and the total volume of pancreatic secretion were all significantly decreased in guinea pigs with overt diabetes, but not in those with latent diabetes mellitus. Pancreatic histologic changes characteristic of both latent and overt diabetes were beta-cell hyperplasia and generalized fatty degeneration of the acini. Only the animals with overt diabetes showed total degranulation and severe vacuolation of theta-cells. The same type of exocrine pancreatic dysfunction observed in guinea pigs with spontaneous overt diabetes mellitus is found in human diabetics, and is particularly common in the juvenile type. The guinea pig, therefore, appears to be a suitable animal model for the study of human juvenile diabetes mellitus.", "contents": "Exocrine pancreatic dysfunction in guinea pigs with diabetes mellitus. In this study, 37 guinea pigs were classified, on the basis of histologic examination of the pancreas, into three categories: nondiabetic, latent diabetic, and overt diabetic. In order to compare the exocrine pancreatic function in these three groups of animals, pancreatic secretion was collected from each animal following an intravenous infusion of secretin and pancreozymin. Pancreatic enzyme activity, bicarbonate concentration, and the total volume of pancreatic secretion were all significantly decreased in guinea pigs with overt diabetes, but not in those with latent diabetes mellitus. Pancreatic histologic changes characteristic of both latent and overt diabetes were beta-cell hyperplasia and generalized fatty degeneration of the acini. Only the animals with overt diabetes showed total degranulation and severe vacuolation of theta-cells. The same type of exocrine pancreatic dysfunction observed in guinea pigs with spontaneous overt diabetes mellitus is found in human diabetics, and is particularly common in the juvenile type. The guinea pig, therefore, appears to be a suitable animal model for the study of human juvenile diabetes mellitus."}
{"id": "PMID:1089837", "title": "Experimental focal glomerular lesions elicited by insoluble immune complexes. Ultrastructural and immunofluorescent studies.", "content": "Injection of small amounts of ferritin intravenously, into the aorta (above the renal arteries) or into the left renal artery of rabbits hyperimmunized against this protein, results in the formation of circulating, insoluble, antigen-antibody complexes. Some of these complexes localize focally in the renal glomerular capillaries, where they elicit severe lesions. The fate of the complexes and the evolution of the lesions have been followed by immunofluorescent and electron microscopic techniques. Within a few hours, the deposition of complexes in the glomeruli resulted in a massive accumulation of neutrophils platelets, and fibrin, sometimes leading to acute focal necroses of some loops. These lesions were quite similar to those developing in the small dermal vessels during the Arthus reaction. Most of the complexes were rapidly phagocytosed and degraded by neutrophils; swelling and proliferation of endothelial and mesangial cells usually followed the acute damage and contributed to the removal of remaining complexes, cell debris, and fibrin deposits. Later, focal areas of mesangial proliferation and sclerosis were observed, containing large amounts of basement membrane-like material and sometimes of collagen fibrils; synechiae and crescent formation were noted in certain places. These observations suggest that the glomerular localization of very large, poorly soluble or insoluble immune complexes may be responsible for the focal glomerular changes seen in association with subacute bacterial endocarditis, with anaphylactoid purpura, or for some of the most severe lesions developing during chronic immune complex diseases.", "contents": "Experimental focal glomerular lesions elicited by insoluble immune complexes. Ultrastructural and immunofluorescent studies. Injection of small amounts of ferritin intravenously, into the aorta (above the renal arteries) or into the left renal artery of rabbits hyperimmunized against this protein, results in the formation of circulating, insoluble, antigen-antibody complexes. Some of these complexes localize focally in the renal glomerular capillaries, where they elicit severe lesions. The fate of the complexes and the evolution of the lesions have been followed by immunofluorescent and electron microscopic techniques. Within a few hours, the deposition of complexes in the glomeruli resulted in a massive accumulation of neutrophils platelets, and fibrin, sometimes leading to acute focal necroses of some loops. These lesions were quite similar to those developing in the small dermal vessels during the Arthus reaction. Most of the complexes were rapidly phagocytosed and degraded by neutrophils; swelling and proliferation of endothelial and mesangial cells usually followed the acute damage and contributed to the removal of remaining complexes, cell debris, and fibrin deposits. Later, focal areas of mesangial proliferation and sclerosis were observed, containing large amounts of basement membrane-like material and sometimes of collagen fibrils; synechiae and crescent formation were noted in certain places. These observations suggest that the glomerular localization of very large, poorly soluble or insoluble immune complexes may be responsible for the focal glomerular changes seen in association with subacute bacterial endocarditis, with anaphylactoid purpura, or for some of the most severe lesions developing during chronic immune complex diseases."}
{"id": "PMID:1089838", "title": "Experimental glomerulonephritis in the guinea pig. II. Ultrastructural lesions of the basement membrane associated with proteinuria.", "content": "Humoral immune processes mediate alterations in glomerular basement membrane (GBM) permeability by two mechanisms. One requires complement and polymorphonuclear leukocytes and the second is complement- and polymorphonuclear leukocyte-independent. The structural basis for enhanced GBM permeability induced by anti-GBM antibody is not clear. Experimental anti-GBM glomerulopathy was induced in guinea pigs by immunization with human GBM in complete Freund's adjuvant. Control animals received injections of complete Freund's adjuvant alone. Light, immunofluorescent, and electron microscopic studies were done on eight heavily proteinuric animals, four immunized nonproteinuric animals, three controls, and two normal animals. All animals that received injections of GBM had intense linear deposits of gamma2 anti-GBM antibody. Complement deposition was not demonstrable in vivo, and anti-GBM antibody deposits did not fix complement in vitro. Histologic abnormalities in proteinuric animals were confined to the GBM, which was of variable density and had a characteristic beaded thickening, with numerous areas of electron lucency most prominent in the outer aspect of GBM in peripheral portions of capillary loops. The inner margin and endothelium were normal. Ultrastructural tracer studies with ferritin demonstrated increased permeability confined to portions of GBM demonstrating ultrastructural lesions. The urine protein excreted by animals with ultrastructural GBM lesions was largely albumin. The absence of complement deposition accompanying anti-GBM antibody deposits in vivo and the unique GBM lesion in this model differ from the findings in nephritis induced by most heterologous nephrotoxic antibodies and suggest that GBM injury in this model is mediated by autologous antibody through complement-independent mechanisms. The selective proteinuria and ultrastructural lesions suggest a derangement in glomerular permeability functionally localized to the epithelial side of the GBM and could reflect an antibody-mediated abnormality in GBM biosynthesis.", "contents": "Experimental glomerulonephritis in the guinea pig. II. Ultrastructural lesions of the basement membrane associated with proteinuria. Humoral immune processes mediate alterations in glomerular basement membrane (GBM) permeability by two mechanisms. One requires complement and polymorphonuclear leukocytes and the second is complement- and polymorphonuclear leukocyte-independent. The structural basis for enhanced GBM permeability induced by anti-GBM antibody is not clear. Experimental anti-GBM glomerulopathy was induced in guinea pigs by immunization with human GBM in complete Freund's adjuvant. Control animals received injections of complete Freund's adjuvant alone. Light, immunofluorescent, and electron microscopic studies were done on eight heavily proteinuric animals, four immunized nonproteinuric animals, three controls, and two normal animals. All animals that received injections of GBM had intense linear deposits of gamma2 anti-GBM antibody. Complement deposition was not demonstrable in vivo, and anti-GBM antibody deposits did not fix complement in vitro. Histologic abnormalities in proteinuric animals were confined to the GBM, which was of variable density and had a characteristic beaded thickening, with numerous areas of electron lucency most prominent in the outer aspect of GBM in peripheral portions of capillary loops. The inner margin and endothelium were normal. Ultrastructural tracer studies with ferritin demonstrated increased permeability confined to portions of GBM demonstrating ultrastructural lesions. The urine protein excreted by animals with ultrastructural GBM lesions was largely albumin. The absence of complement deposition accompanying anti-GBM antibody deposits in vivo and the unique GBM lesion in this model differ from the findings in nephritis induced by most heterologous nephrotoxic antibodies and suggest that GBM injury in this model is mediated by autologous antibody through complement-independent mechanisms. The selective proteinuria and ultrastructural lesions suggest a derangement in glomerular permeability functionally localized to the epithelial side of the GBM and could reflect an antibody-mediated abnormality in GBM biosynthesis."}
{"id": "PMID:1089839", "title": "Subacute sclerosing panencephalitis virus in immunosuppressed adult hamster.", "content": "A hamster-adapted subacute sclerosing panencephalitis agent (HBS virus) produced a subclinical, nonlethal, and cell-associated encephalitis after intracerebral inoculation of adult hamsters. When adult hamsters were immunosuppressed by intraperitoneal injections of rabbit origin anti-hamster lymphocyte serum for varying periods of time before and after intracerebral challenge with HBS virus, more than 50 per cent died in all experiments. Large titers of cell-free virus developed acutely in all animals and were maintained in continuously immunosuppressed hamsters, all but one of which died within 21 days. When animals were given transient anti-hamster lymphocyte serum treatment, those surviving for 21 to 54 days always displayed serum measles antibodies, and, from such animals, highly cell-associated virus could often be isolated from the brain. In such animals and in some others from which no virus could be isolated, the presence of measles antigen or inclusion cell pathology was noted in the brain. The neuropathology was characterized early by focal necrosis; small, multinucleated, ependymal giant cells; and minimal inflammatory reactions. Chronically, it was represented by mild perivascular inflammation, plasma cell accumulations, and inclusion-bearing cells. These studies indicate that it is the host immune response, rather than intrinsic central nervous system resistance to virus infection, which changes with age and thereby increases the chance of survival. The results suggest that antibodies modify the virus, making it more cell-associated or defective and thus enhancing its persistence in the immune host.", "contents": "Subacute sclerosing panencephalitis virus in immunosuppressed adult hamster. A hamster-adapted subacute sclerosing panencephalitis agent (HBS virus) produced a subclinical, nonlethal, and cell-associated encephalitis after intracerebral inoculation of adult hamsters. When adult hamsters were immunosuppressed by intraperitoneal injections of rabbit origin anti-hamster lymphocyte serum for varying periods of time before and after intracerebral challenge with HBS virus, more than 50 per cent died in all experiments. Large titers of cell-free virus developed acutely in all animals and were maintained in continuously immunosuppressed hamsters, all but one of which died within 21 days. When animals were given transient anti-hamster lymphocyte serum treatment, those surviving for 21 to 54 days always displayed serum measles antibodies, and, from such animals, highly cell-associated virus could often be isolated from the brain. In such animals and in some others from which no virus could be isolated, the presence of measles antigen or inclusion cell pathology was noted in the brain. The neuropathology was characterized early by focal necrosis; small, multinucleated, ependymal giant cells; and minimal inflammatory reactions. Chronically, it was represented by mild perivascular inflammation, plasma cell accumulations, and inclusion-bearing cells. These studies indicate that it is the host immune response, rather than intrinsic central nervous system resistance to virus infection, which changes with age and thereby increases the chance of survival. The results suggest that antibodies modify the virus, making it more cell-associated or defective and thus enhancing its persistence in the immune host."}
{"id": "PMID:1089840", "title": "Effect of beta-aminopropionitrile on in vitro bone lipid synthesis.", "content": "The effect of beta-aminopropionitrile (BAPN) on lipid synthesis and 14CO2 production from 14C-acetate by rat calvariae in vitro was investigated by biochemical, histochemical, and autoradiographic techniques. Exposure of bones to 5 mM BAPN throughout a 5-hour incubation period resulted in little effect on lipid synthesis when compared with control bones. Higher BAPN concentrations, up to 100 mM, during this short incubation period resulted in enhanced lipid radioactivity for most of the identified lipid classes. Longer preincubation periods of 20 hours and low BAPN (20 mM) concentrations resulted in enhanced lipogenesis. As BAPN concentrations above 20 mM were employed, lipogenesis decreased to such a level that some lipid classes failed to become labeled with 14C-acetate. 14CO2 production from 14C-acetate by a rat calvariae was affected by BAPN concentration and incubation time in a pattern similar to that of lipid synthesis. Histochemistry demonstrated neutral fat in the osteogenic cells and phospholipids in osteoblasts and the osteoid matrix. Autoradiographic localization of 14C-acetate revealed that the radioactivity was in the osteogenic cells and that this activity was markedly increased after pretreatment of the calvariae with BAPN. This study shows that some concentrations of BAPN enhance lipogenesis by rat calvariae and supports the earlier suggestion that lipids play a role in biologic calcification.", "contents": "Effect of beta-aminopropionitrile on in vitro bone lipid synthesis. The effect of beta-aminopropionitrile (BAPN) on lipid synthesis and 14CO2 production from 14C-acetate by rat calvariae in vitro was investigated by biochemical, histochemical, and autoradiographic techniques. Exposure of bones to 5 mM BAPN throughout a 5-hour incubation period resulted in little effect on lipid synthesis when compared with control bones. Higher BAPN concentrations, up to 100 mM, during this short incubation period resulted in enhanced lipid radioactivity for most of the identified lipid classes. Longer preincubation periods of 20 hours and low BAPN (20 mM) concentrations resulted in enhanced lipogenesis. As BAPN concentrations above 20 mM were employed, lipogenesis decreased to such a level that some lipid classes failed to become labeled with 14C-acetate. 14CO2 production from 14C-acetate by a rat calvariae was affected by BAPN concentration and incubation time in a pattern similar to that of lipid synthesis. Histochemistry demonstrated neutral fat in the osteogenic cells and phospholipids in osteoblasts and the osteoid matrix. Autoradiographic localization of 14C-acetate revealed that the radioactivity was in the osteogenic cells and that this activity was markedly increased after pretreatment of the calvariae with BAPN. This study shows that some concentrations of BAPN enhance lipogenesis by rat calvariae and supports the earlier suggestion that lipids play a role in biologic calcification."}
{"id": "PMID:1089834", "title": "[Biochemical changes in skeletal muscles during hypokinesia and possible means for counteracting them].", "content": "The changes in the metabolism of proteins, carbohydrates, fats, water and minerals as well as in the acitvity of enzymes of skeletal muscles during hypokinesia are discussed. The methods used to reverse metabolic disorders in skeletal muscles are recommended and evaluated. Among the suggested methods-the diet with predominant proteins, hypoxia, anabolic steroids and physical exercises-the latter seem to be most promising.", "contents": "[Biochemical changes in skeletal muscles during hypokinesia and possible means for counteracting them]. The changes in the metabolism of proteins, carbohydrates, fats, water and minerals as well as in the acitvity of enzymes of skeletal muscles during hypokinesia are discussed. The methods used to reverse metabolic disorders in skeletal muscles are recommended and evaluated. Among the suggested methods-the diet with predominant proteins, hypoxia, anabolic steroids and physical exercises-the latter seem to be most promising."}
{"id": "PMID:1089841", "title": "Immunoglobulin A glomerulonephritis: a clinicopathologic study.", "content": "Renal biopsies from 10 patients staining predominantly for immunoglobulin A are reviewed. Historically, nine of 10 patients were less than 32 years of age; they all presented with either microscopic or gross hematuria. The initial creatinine clearances demonstrated good function in all patients; only one patient experienced a progressive decline in the creatinine clearance. Similarly, proteinuria was mild. The light and electron microscopic findings presented a variable pattern, including a group with changes confined to the mesangium, another group also having glomerular basement membrane changes, and one patient with severe, progressive glomerular sclerosis. Immunogluorescence demonstrated large amounts of IgA predominantly within the mesangium but occasionally involving the peripheral capillary loops. Serum IgA levels were elevated in six of eight patients tested, and two of five patients had elevated nasal IgA concentrations. These data suggest that there is an immunologic entity, IgA glomerulonephritis, characterized by the above clincial findings in association with elevated serum and occasionally nasal IgA levels, but that the pathologic findings are highly variable. Neither the mechanism nor the particular pathogenetic significance of the raised IgA levels is presently known. The similarities of this entity to the reported findings in anaphylactoid purpura are mentioned.", "contents": "Immunoglobulin A glomerulonephritis: a clinicopathologic study. Renal biopsies from 10 patients staining predominantly for immunoglobulin A are reviewed. Historically, nine of 10 patients were less than 32 years of age; they all presented with either microscopic or gross hematuria. The initial creatinine clearances demonstrated good function in all patients; only one patient experienced a progressive decline in the creatinine clearance. Similarly, proteinuria was mild. The light and electron microscopic findings presented a variable pattern, including a group with changes confined to the mesangium, another group also having glomerular basement membrane changes, and one patient with severe, progressive glomerular sclerosis. Immunogluorescence demonstrated large amounts of IgA predominantly within the mesangium but occasionally involving the peripheral capillary loops. Serum IgA levels were elevated in six of eight patients tested, and two of five patients had elevated nasal IgA concentrations. These data suggest that there is an immunologic entity, IgA glomerulonephritis, characterized by the above clincial findings in association with elevated serum and occasionally nasal IgA levels, but that the pathologic findings are highly variable. Neither the mechanism nor the particular pathogenetic significance of the raised IgA levels is presently known. The similarities of this entity to the reported findings in anaphylactoid purpura are mentioned."}
{"id": "PMID:1089842", "title": "Cytoplasmic contractile apparatus in aortic endothelial cells of hypertensive rats.", "content": "Hypertension was produced in male Wistar rats (150 gm. body weight) by complete ligature of the aorta between the renal arteries. Electron  microscopic examination revealed that 1 week later the amount of cytoplasmic microfilaments in the endothelial cells of the aortic segment above the coarctation (mean blood pressure 160 mm. Hg) was strikingly increased as compared with normal animals. The endothelial cells in the segment below the coarctation (mean blood pressure 25 mm. Hg) contained few filaments and were similar to the cells in the aortic endothelium in controls (mean blood pressure 105 mm. Hg). Microfilaments measured 40 to 70 angstrom in diameter and were mostly located close to the endothelial clefts, where they formed longitudinal bundles or a network. The bundles of microfilaments contained electron-dense areas similar to the \"attachment sites\" of the underlying smooth muscle. By using en face preparations of aortic endothelial cells treated with antiactin autoantibodies (AAA) followed by anti-human IgG, it was seen that in hypertensive animals the cells above the ligature were intensely fluorescent when compared with those of the aortic portion below the ligature or those of the controls. The fluorescence was abolished after incubation of the AAA sera with thrombosthenin A. The correlation between electron microscopic and immunologic findings suggests that the microfilaments present in the endothelial cells of hypertensive animals are composed, at least in part, of actin. Endothelial cells so modified may play a role in permeability or may be related to other phenomena such as electrotonic coupling and synchronized contraction of aortic cells during hypertension.", "contents": "Cytoplasmic contractile apparatus in aortic endothelial cells of hypertensive rats. Hypertension was produced in male Wistar rats (150 gm. body weight) by complete ligature of the aorta between the renal arteries. Electron  microscopic examination revealed that 1 week later the amount of cytoplasmic microfilaments in the endothelial cells of the aortic segment above the coarctation (mean blood pressure 160 mm. Hg) was strikingly increased as compared with normal animals. The endothelial cells in the segment below the coarctation (mean blood pressure 25 mm. Hg) contained few filaments and were similar to the cells in the aortic endothelium in controls (mean blood pressure 105 mm. Hg). Microfilaments measured 40 to 70 angstrom in diameter and were mostly located close to the endothelial clefts, where they formed longitudinal bundles or a network. The bundles of microfilaments contained electron-dense areas similar to the \"attachment sites\" of the underlying smooth muscle. By using en face preparations of aortic endothelial cells treated with antiactin autoantibodies (AAA) followed by anti-human IgG, it was seen that in hypertensive animals the cells above the ligature were intensely fluorescent when compared with those of the aortic portion below the ligature or those of the controls. The fluorescence was abolished after incubation of the AAA sera with thrombosthenin A. The correlation between electron microscopic and immunologic findings suggests that the microfilaments present in the endothelial cells of hypertensive animals are composed, at least in part, of actin. Endothelial cells so modified may play a role in permeability or may be related to other phenomena such as electrotonic coupling and synchronized contraction of aortic cells during hypertension."}
{"id": "PMID:1089843", "title": "Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigens. II. Influence of medication with prednisone and azathioprine: a histologic and immunohistologic study at the light microscopic and the ultrastructural level.", "content": "In this paper, the effect of the administration of prednisone and azathioprine on an experimental immune complex glomerulonephritis is studied. During the observation period of 3 months, it appeared that prednisone had no effect either on the morphology of glomerulonephritis or on the proteinuria caused by it. Azathioprine, especially when given 4 days before or simultaneously with the induction of the experimental immune complex glomerulonephritis, showed a clear effect on the morphology of the glomerulonephritis as well as on the proteinuria. The damage to the glomerular basement membrane as a result of deposition of immune complexes was less and the proteinuria was significantly lower. The effect of azathioprine was not present when given 10 days after the induction of glomerulonephritis, at a time when the autologous phase had already developed.", "contents": "Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigens. II. Influence of medication with prednisone and azathioprine: a histologic and immunohistologic study at the light microscopic and the ultrastructural level. In this paper, the effect of the administration of prednisone and azathioprine on an experimental immune complex glomerulonephritis is studied. During the observation period of 3 months, it appeared that prednisone had no effect either on the morphology of glomerulonephritis or on the proteinuria caused by it. Azathioprine, especially when given 4 days before or simultaneously with the induction of the experimental immune complex glomerulonephritis, showed a clear effect on the morphology of the glomerulonephritis as well as on the proteinuria. The damage to the glomerular basement membrane as a result of deposition of immune complexes was less and the proteinuria was significantly lower. The effect of azathioprine was not present when given 10 days after the induction of glomerulonephritis, at a time when the autologous phase had already developed."}
{"id": "PMID:1089846", "title": "Extrathoracic bypass for stenosis of innominate artery.", "content": "Stenosis of the innominate artery usually occurs in older patients in whom the risks of conventional, corrective procedures such as transthoracic endarterectomy or aorto-innominate arterial bypass grafting are quite high. An axillo-axillary bypass procedure was performed in a high-risk patient with innominate arterial stenosis who had repeated episodes of transient cerebral ischemia due to decreased blood flow through the right carotid artery and reversal of blood flow through the right vertebral artery. Postoperatively, he has had dramatic improvement in his symptoms. Because of its simplicity, avoidance of major thoracotomy, avoidance of temporary occlusion of the carotid artery, and excellent late results, axillo-axillary bypass grafting is being proposed as the procedure of choice for stenosis of the innominate artery.", "contents": "Extrathoracic bypass for stenosis of innominate artery. Stenosis of the innominate artery usually occurs in older patients in whom the risks of conventional, corrective procedures such as transthoracic endarterectomy or aorto-innominate arterial bypass grafting are quite high. An axillo-axillary bypass procedure was performed in a high-risk patient with innominate arterial stenosis who had repeated episodes of transient cerebral ischemia due to decreased blood flow through the right carotid artery and reversal of blood flow through the right vertebral artery. Postoperatively, he has had dramatic improvement in his symptoms. Because of its simplicity, avoidance of major thoracotomy, avoidance of temporary occlusion of the carotid artery, and excellent late results, axillo-axillary bypass grafting is being proposed as the procedure of choice for stenosis of the innominate artery."}
{"id": "PMID:1089847", "title": "Hemodynamic observations in the early period after human heart transplantation.", "content": "Serial hemodynamic measurements, including determination of cardiac output by the Fick technique, were obtained in 10 human cardiac recipients for intervals up to 38 days after transplantation. Immediately postoperatively, donor cardiac output was severely depressed because of limitation of stroke volume. Spontaneous recovery of cardiac output and stroke volume then occurred gradually over the first 4 postoperative days to normal or nearly normal levels. Rate augmentation by arterial and/or ventricular pacing early after transplantation had little effect on donor heart performance, but isoproterenol caused significant enhancement of graft function and is now used routinely in postoperative management. Serial hemodynamic monitoring proved to be of little use in the prediction or confirmation of acute graft rejection episodes.", "contents": "Hemodynamic observations in the early period after human heart transplantation. Serial hemodynamic measurements, including determination of cardiac output by the Fick technique, were obtained in 10 human cardiac recipients for intervals up to 38 days after transplantation. Immediately postoperatively, donor cardiac output was severely depressed because of limitation of stroke volume. Spontaneous recovery of cardiac output and stroke volume then occurred gradually over the first 4 postoperative days to normal or nearly normal levels. Rate augmentation by arterial and/or ventricular pacing early after transplantation had little effect on donor heart performance, but isoproterenol caused significant enhancement of graft function and is now used routinely in postoperative management. Serial hemodynamic monitoring proved to be of little use in the prediction or confirmation of acute graft rejection episodes."}
{"id": "PMID:1089848", "title": "Surgical repair of total cleft sternum.", "content": "There are two objectives in the surgical repair of congenital total cleft sternum:(1) to afford normal bony protection to the mediastinal structures and (2) to provide stability of the anterior thorax so that respiratory function and circulation can be maintained physiologically within normal pressure relationships. A case is presented, and a new method for repair of this life-threatening congenital anomaly is described.", "contents": "Surgical repair of total cleft sternum. There are two objectives in the surgical repair of congenital total cleft sternum:(1) to afford normal bony protection to the mediastinal structures and (2) to provide stability of the anterior thorax so that respiratory function and circulation can be maintained physiologically within normal pressure relationships. A case is presented, and a new method for repair of this life-threatening congenital anomaly is described."}
{"id": "PMID:1089849", "title": "Evaluation of an outpatient pediatric practice through the use of consumer questionnaires.", "content": "Two questionnaires were given to parents accompanying children to an out-patient pediatric center. The first was given to all Ss directly after a care-receiving visit; the second was given one week after the visit only to Ss accompanying Ps receiving throat cultures. Care was judged extremely favorably in both instances. Quality of care measures intercorrelated very highly. No strong relationship was found between length of illness or illness versus absence and rated quality of care received.", "contents": "Evaluation of an outpatient pediatric practice through the use of consumer questionnaires. Two questionnaires were given to parents accompanying children to an out-patient pediatric center. The first was given to all Ss directly after a care-receiving visit; the second was given one week after the visit only to Ss accompanying Ps receiving throat cultures. Care was judged extremely favorably in both instances. Quality of care measures intercorrelated very highly. No strong relationship was found between length of illness or illness versus absence and rated quality of care received."}
{"id": "PMID:1089851", "title": "The significance of positive margins in hemilaryngectomy specimens.", "content": "This clinicopathologic study was undertaken to determine the meaning of surgical margins \"involved\" with carcinoma. The fate of hemilaryngectomy patients whose specimens had this finding was comparared with that of patients who had \"uninvolved\" margins. A consecutive series of 111 hemilaryngectomies performed for previously untreated invasive epidermoid carcinoma was analyzed. Serial step sections in a longitudinal plane were available for re-examination and re-evaluation of the surgical margins in each case. Clinical follow-up on every patient was current through 1972. Thirty-nine patients had cancer involvement of a margin in the hemilaryngectomy specimen. None of these patients received any immediate therapy but were followed only. Seven of these patients (18 percent) subsequently developed a biopsy proven local recurrence. Four of the 72 patients (6 percent) with uninvolved margins developed a local recurrence. The site of the positive margin in the specimen was compared with the clinical site of recurrence. The seven local recurrences in patients with positive margins were treated with full course irradiation or total laryngectomy. All of these patients are alive and free of cancer or have died of other causes without evidence of cancer. Of the four local recurrences in patients with negative margins one died of cancer; two are living and well, and one died of other causes. This study provides evidence to support the conservative management of those hemilaryngectomy patients who have involved margins in the resection specimen. No immediate treatment is required. Careful follow-up is indicated with 18 percent chance of clinical recurrence. These biopsy proven recurrences can then be successfully treated with total laryngectomy or full course irradiation. Utilizing this approach none of the 39 patients with involved margins died of cancer in the 5 to 12-year follow-up period.", "contents": "The significance of positive margins in hemilaryngectomy specimens. This clinicopathologic study was undertaken to determine the meaning of surgical margins \"involved\" with carcinoma. The fate of hemilaryngectomy patients whose specimens had this finding was comparared with that of patients who had \"uninvolved\" margins. A consecutive series of 111 hemilaryngectomies performed for previously untreated invasive epidermoid carcinoma was analyzed. Serial step sections in a longitudinal plane were available for re-examination and re-evaluation of the surgical margins in each case. Clinical follow-up on every patient was current through 1972. Thirty-nine patients had cancer involvement of a margin in the hemilaryngectomy specimen. None of these patients received any immediate therapy but were followed only. Seven of these patients (18 percent) subsequently developed a biopsy proven local recurrence. Four of the 72 patients (6 percent) with uninvolved margins developed a local recurrence. The site of the positive margin in the specimen was compared with the clinical site of recurrence. The seven local recurrences in patients with positive margins were treated with full course irradiation or total laryngectomy. All of these patients are alive and free of cancer or have died of other causes without evidence of cancer. Of the four local recurrences in patients with negative margins one died of cancer; two are living and well, and one died of other causes. This study provides evidence to support the conservative management of those hemilaryngectomy patients who have involved margins in the resection specimen. No immediate treatment is required. Careful follow-up is indicated with 18 percent chance of clinical recurrence. These biopsy proven recurrences can then be successfully treated with total laryngectomy or full course irradiation. Utilizing this approach none of the 39 patients with involved margins died of cancer in the 5 to 12-year follow-up period."}
{"id": "PMID:1089852", "title": "Certain effects of adenoidectomy of Eustachian tube ventilatory function.", "content": "In an effort to develop a simple and accurate method to identify children in whom adenoidectomy might prevent otitis media, the ventilatory function of the Eustachian tube was assessed by a manometric technique. Nasal pressures during swallowing were also determined in some. The study group consisted of 23 children with otitis media in whom tympanostomy tubes had been inserted. All were judged clinically and roentgenographically to have prominent adenoids. Inflation-deflation Eustachian tube ventilation studies were obtained in 36 ears that remained intubated, aerated and dry both before and eight weeks after adenoidectomy. Fifteen of the 36 (42 percent) ears had improvement in Eustachian tube ventilatory function postadenoidectomy which was attributed to relief of extrinsic mechanical obstruction of the tube. In the remaining 21 (58 percent) ears in which Eustachian tube function was not improved, mechanical obstruction was not apparent preoperatively. The effect of nasopharyngeal pressures on a pliant Eustachian tube (Toynbee phenomenon) due to obstruction of the posterior nasal choanae by the adenoid mass was suggested as a possible cause of functional Eustachian tube obstruction. In several instances in which preadenoidectomy mechanical obstruction of the Eustachian tube was not demonstrated, the tube appeared to have been made more pliant by the operation. This increase in compliance of the Eustachian tube was attributed to loss of adenoid support of the tube in the fossa of Rosenmuller. From this study, preliminary recommendations for selection of patients for adenoidectomy are the following: 1. Eustachian tube ventilation function tests in a dry, intubated middle ear; 2. if extrinsic mechanical obstruction of the Eustachian tube is present and chronic inflammation is absent, adenoidectomy will probably improve Eustachian tube function. The surgical technique should include adequate removal of the adenoid tissue in the fossa of Rosenmuller; 3. if the Eustachian tube does not appear to be mechanically obstructed, the adenoids should not be removed unless functional obstruction is suspected due to obstruction of the posterior nasal choanae. Adenoid tissue within the fossa of Rosenmuller should not be removed when such circumstances exist; and 4. in the abscence of obstructive adenoids to the nasal choanae or Eustachian tube, adenoidectomy probably will not improve Eustachian tube function and could make it worse. A more rational and effective approach to adenoidectomy for the prevention of otitis media in children may be possible through this type of preoperative evaluation.", "contents": "Certain effects of adenoidectomy of Eustachian tube ventilatory function. In an effort to develop a simple and accurate method to identify children in whom adenoidectomy might prevent otitis media, the ventilatory function of the Eustachian tube was assessed by a manometric technique. Nasal pressures during swallowing were also determined in some. The study group consisted of 23 children with otitis media in whom tympanostomy tubes had been inserted. All were judged clinically and roentgenographically to have prominent adenoids. Inflation-deflation Eustachian tube ventilation studies were obtained in 36 ears that remained intubated, aerated and dry both before and eight weeks after adenoidectomy. Fifteen of the 36 (42 percent) ears had improvement in Eustachian tube ventilatory function postadenoidectomy which was attributed to relief of extrinsic mechanical obstruction of the tube. In the remaining 21 (58 percent) ears in which Eustachian tube function was not improved, mechanical obstruction was not apparent preoperatively. The effect of nasopharyngeal pressures on a pliant Eustachian tube (Toynbee phenomenon) due to obstruction of the posterior nasal choanae by the adenoid mass was suggested as a possible cause of functional Eustachian tube obstruction. In several instances in which preadenoidectomy mechanical obstruction of the Eustachian tube was not demonstrated, the tube appeared to have been made more pliant by the operation. This increase in compliance of the Eustachian tube was attributed to loss of adenoid support of the tube in the fossa of Rosenmuller. From this study, preliminary recommendations for selection of patients for adenoidectomy are the following: 1. Eustachian tube ventilation function tests in a dry, intubated middle ear; 2. if extrinsic mechanical obstruction of the Eustachian tube is present and chronic inflammation is absent, adenoidectomy will probably improve Eustachian tube function. The surgical technique should include adequate removal of the adenoid tissue in the fossa of Rosenmuller; 3. if the Eustachian tube does not appear to be mechanically obstructed, the adenoids should not be removed unless functional obstruction is suspected due to obstruction of the posterior nasal choanae. Adenoid tissue within the fossa of Rosenmuller should not be removed when such circumstances exist; and 4. in the abscence of obstructive adenoids to the nasal choanae or Eustachian tube, adenoidectomy probably will not improve Eustachian tube function and could make it worse. A more rational and effective approach to adenoidectomy for the prevention of otitis media in children may be possible through this type of preoperative evaluation."}
{"id": "PMID:1089853", "title": "The inverted Schneiderian papilloma: a clinical and pathological study.", "content": "Inverted papillomas which arise from the lining membranes of the nose and paranasal sinuses are relatively unfamiliar lesions which have been reported in the literature under a variety of titles. The uncertainly surrounding their etiology, their relationship to nasal polyps and their malignant potential have resulted in an ill-defined clinical approach to their management. The designation Inverted Schneiderian Papilloma is suggested as an appropriate title that best conveys the qualities of inversion, location and distinctiveness of character. The characteristic microscopic feature is the increase in thickness of the covering epithelium with extensive invasion of this hyperplastic epithelium into the underlying stroma. In the absence of a better explanation of the origin, the tumor should be considered a true epithelial neoplasm. The clinical features in 24 previously unreported cases are presented. The most common presenting complaints are nasal obstruction and epistaxis. The common site of origin is the lateral nasal wall in the region of the middle meatus and ethmoid cells. In no instance was an isolated lesion of the maxillary, frontal or sphenoid sinus present. The most characteristic attributes of the tumor were its tendency to recur, its destructive capacity and its propensity to be associated with malignancy. The common radiographic abnormality on routine sinus films was unilateral opacification of the sinuses and nasal airway. Tomography is helpful in defining the extent of the lesion and in selecting an appropriate surgical approach. A philosophy of management has evolved based on the experiences gained from these 24 patients, combined with a review of the experience of others and a study of the regional anatomy. Surgical excision is the treatment of choice. A bold surgical approach has been used for tumors involving the lateral nasal wall and paranasal sinuses. A lateral rhinotomy incision is employed and when necessary, this exposure is increased by extending the incision of split the upper lip and reflect the cheek flap as is customarily done with the Weber-Ferfusson incision. Fifteen patients have been followed for more than two years and the results have been excellent with the exception of one patient who later developed an invasive squamous carcinoma. An associated malignancy was found in 12.5 percent of the cases.", "contents": "The inverted Schneiderian papilloma: a clinical and pathological study. Inverted papillomas which arise from the lining membranes of the nose and paranasal sinuses are relatively unfamiliar lesions which have been reported in the literature under a variety of titles. The uncertainly surrounding their etiology, their relationship to nasal polyps and their malignant potential have resulted in an ill-defined clinical approach to their management. The designation Inverted Schneiderian Papilloma is suggested as an appropriate title that best conveys the qualities of inversion, location and distinctiveness of character. The characteristic microscopic feature is the increase in thickness of the covering epithelium with extensive invasion of this hyperplastic epithelium into the underlying stroma. In the absence of a better explanation of the origin, the tumor should be considered a true epithelial neoplasm. The clinical features in 24 previously unreported cases are presented. The most common presenting complaints are nasal obstruction and epistaxis. The common site of origin is the lateral nasal wall in the region of the middle meatus and ethmoid cells. In no instance was an isolated lesion of the maxillary, frontal or sphenoid sinus present. The most characteristic attributes of the tumor were its tendency to recur, its destructive capacity and its propensity to be associated with malignancy. The common radiographic abnormality on routine sinus films was unilateral opacification of the sinuses and nasal airway. Tomography is helpful in defining the extent of the lesion and in selecting an appropriate surgical approach. A philosophy of management has evolved based on the experiences gained from these 24 patients, combined with a review of the experience of others and a study of the regional anatomy. Surgical excision is the treatment of choice. A bold surgical approach has been used for tumors involving the lateral nasal wall and paranasal sinuses. A lateral rhinotomy incision is employed and when necessary, this exposure is increased by extending the incision of split the upper lip and reflect the cheek flap as is customarily done with the Weber-Ferfusson incision. Fifteen patients have been followed for more than two years and the results have been excellent with the exception of one patient who later developed an invasive squamous carcinoma. An associated malignancy was found in 12.5 percent of the cases."}
{"id": "PMID:1089874", "title": "Failure of high dose 5-iodo-2'-deoxyuridine in the therapy of herpes simplex virus encephalitis. Evidence of unacceptable toxicity.", "content": "Two placebo-controlled double-blind studies were initiated to evaluate the therapeutic efficacy of 5-iodo-2-deoxyuridine (idoxuridine) in biopsy-provedcases of herpes simplex virus encephalitis. Twelve patients who on clinical grounds were thought to have herepes simplex virus encephalitis underwent brain biopsy; six of these patients were proved to have this disease, three were considered probable cases,and three were considered doubtful. The patients with proved or probably herpes simplex virus encephalitis were treated with parenteral idoxuridine (or placebo) at a dose of 100 mg per kiogram per day for five days. The occurrence of unacceptable myelosupperssion and the failure of idoxuridine therapy to prevent death led to the premature termination of both studies.", "contents": "Failure of high dose 5-iodo-2'-deoxyuridine in the therapy of herpes simplex virus encephalitis. Evidence of unacceptable toxicity. Two placebo-controlled double-blind studies were initiated to evaluate the therapeutic efficacy of 5-iodo-2-deoxyuridine (idoxuridine) in biopsy-provedcases of herpes simplex virus encephalitis. Twelve patients who on clinical grounds were thought to have herepes simplex virus encephalitis underwent brain biopsy; six of these patients were proved to have this disease, three were considered probable cases,and three were considered doubtful. The patients with proved or probably herpes simplex virus encephalitis were treated with parenteral idoxuridine (or placebo) at a dose of 100 mg per kiogram per day for five days. The occurrence of unacceptable myelosupperssion and the failure of idoxuridine therapy to prevent death led to the premature termination of both studies."}
{"id": "PMID:1089875", "title": "Treatment of gallstones with chenodeoxycholic acid and phenobarbital.", "content": "In a controlled trial, 36 patients with asymptomatic radiolucent gallstones were treated with chenodeoxycholic acid, 750 mg per day, phenobarbital, 180 mg per day, combination of both drugs, and placebo. After one year, chenodeoxycholic acid, phenobarbital and the combination, but not placebo, significantly decreased biliary cholesterol saturation. The effect was significantly greater with chenodeoxycholic acid and the combination than with phenobarbital. Gallstones size decreased more than 50 per cent in nine of 20 patients receiving chenodeoxycholic acid, either alone or combined with phenobarbital, but in no patient receiving only phenobarbital or placebo. Gallstones disappeared completely in tow patients. Abnormalities in liver-function tests in thriee of 36 patients and in five of 16 liver biopsies, occured with equal frequency in the four treatment groups. Thus, after one year, phenobarbital alone was ineffective in gallstone dissolution. Chenodeoxycholic acid alone or combined with phenobarbital, however, offered a partially effective and safe treatment for asymptomatic radiolucent gallstones.", "contents": "Treatment of gallstones with chenodeoxycholic acid and phenobarbital. In a controlled trial, 36 patients with asymptomatic radiolucent gallstones were treated with chenodeoxycholic acid, 750 mg per day, phenobarbital, 180 mg per day, combination of both drugs, and placebo. After one year, chenodeoxycholic acid, phenobarbital and the combination, but not placebo, significantly decreased biliary cholesterol saturation. The effect was significantly greater with chenodeoxycholic acid and the combination than with phenobarbital. Gallstones size decreased more than 50 per cent in nine of 20 patients receiving chenodeoxycholic acid, either alone or combined with phenobarbital, but in no patient receiving only phenobarbital or placebo. Gallstones disappeared completely in tow patients. Abnormalities in liver-function tests in thriee of 36 patients and in five of 16 liver biopsies, occured with equal frequency in the four treatment groups. Thus, after one year, phenobarbital alone was ineffective in gallstone dissolution. Chenodeoxycholic acid alone or combined with phenobarbital, however, offered a partially effective and safe treatment for asymptomatic radiolucent gallstones."}
{"id": "PMID:1089879", "title": "Relation between quality assessment and utilization review in a functioning PSRO.", "content": "The Utah State Medical Association, through its peer-review foundation, the Utah Professional Review Organization, has implemented an integrated system of utilization reveiw and quality assessment linked to a program of continuing education.  Nurse co-ordinators, employed by the Organization, screen all hospitalized patients covered by Titles XVIII, XIX and V as well as by subscribing private insurers. Approximately 10 per cent of hospitalized patients do not meet peer-generated criteria for admission review or length of stay and are screened out for peer review,  The nurse co-ordinators collect data for retrospective quality assessment, identifying targets for continuing medical education.  Preliminary data support the cost effectiveness of such a review program.  Continuing qualtiy audit will test the effectiveness of education programs.", "contents": "Relation between quality assessment and utilization review in a functioning PSRO. The Utah State Medical Association, through its peer-review foundation, the Utah Professional Review Organization, has implemented an integrated system of utilization reveiw and quality assessment linked to a program of continuing education.  Nurse co-ordinators, employed by the Organization, screen all hospitalized patients covered by Titles XVIII, XIX and V as well as by subscribing private insurers. Approximately 10 per cent of hospitalized patients do not meet peer-generated criteria for admission review or length of stay and are screened out for peer review,  The nurse co-ordinators collect data for retrospective quality assessment, identifying targets for continuing medical education.  Preliminary data support the cost effectiveness of such a review program.  Continuing qualtiy audit will test the effectiveness of education programs."}
{"id": "PMID:1089883", "title": "Severe combined immunodeficiency and adenosine deaminase deficiency.", "content": "Because others had described a lack of the enzyme adenosine deaminase as associated with severe combined immunodeficiency, we surveyed kindreds with infants affected with such an immunodeficiency. Three infants in two families with severe combined immunodeficiency were found to have no detectable erythrocyte adenosine deaminase. Eleven family members heterozygous for adenosine deaminase deficiency were encountered among the first-degree relatives; adenosine deaminase deficiency and severe combined immunodeficiency were associated and inherited as autosomal recessive traits in both kindreds. Successful bone-marrow transplantation was carried out in two of these infants. Normal immunologic function was established in both children, but the deficiency of adenosine deaminase persisted in their erythrocytes. The enzyme deficiency did not impair the successful establishment of normal humoral and cellular immunity by transplants of bone-marrow cells from siblings who were either normal or heterozygous for adenosine deaminase deficiency.", "contents": "Severe combined immunodeficiency and adenosine deaminase deficiency. Because others had described a lack of the enzyme adenosine deaminase as associated with severe combined immunodeficiency, we surveyed kindreds with infants affected with such an immunodeficiency. Three infants in two families with severe combined immunodeficiency were found to have no detectable erythrocyte adenosine deaminase. Eleven family members heterozygous for adenosine deaminase deficiency were encountered among the first-degree relatives; adenosine deaminase deficiency and severe combined immunodeficiency were associated and inherited as autosomal recessive traits in both kindreds. Successful bone-marrow transplantation was carried out in two of these infants. Normal immunologic function was established in both children, but the deficiency of adenosine deaminase persisted in their erythrocytes. The enzyme deficiency did not impair the successful establishment of normal humoral and cellular immunity by transplants of bone-marrow cells from siblings who were either normal or heterozygous for adenosine deaminase deficiency."}
{"id": "PMID:1089884", "title": "Value of early ambulation in patients with and without complications after acute myocardial infarction.", "content": "A prospective, controlled, randomized study was done to compare the effect of early and late ambulation in hospitalized patients with acute myocardial infarction. All patients surviving longer than the first five days were studied; 64 patients were mobilized on day six and discharged on day 12, and 65 were mobilized on day 13 and discharged on day 19. Follow-up observation lasted from six to 52 weeks. Of patients without complications until day six, eight out of 32 in the early and 16 of 35 in the late groups manifested complications during the follow-up period (p smaller than 0.05). Of those who had complications before day six, seven of 32 and 26 of 30 still had or acquired new complications until last seen (p small than 0.0001). The number of serious complications in the two groups was eight and 24 respectively (p smaller than 0.001). We conclude that early ambulation is beneficial irrespective of complications on admission.", "contents": "Value of early ambulation in patients with and without complications after acute myocardial infarction. A prospective, controlled, randomized study was done to compare the effect of early and late ambulation in hospitalized patients with acute myocardial infarction. All patients surviving longer than the first five days were studied; 64 patients were mobilized on day six and discharged on day 12, and 65 were mobilized on day 13 and discharged on day 19. Follow-up observation lasted from six to 52 weeks. Of patients without complications until day six, eight out of 32 in the early and 16 of 35 in the late groups manifested complications during the follow-up period (p smaller than 0.05). Of those who had complications before day six, seven of 32 and 26 of 30 still had or acquired new complications until last seen (p small than 0.0001). The number of serious complications in the two groups was eight and 24 respectively (p smaller than 0.001). We conclude that early ambulation is beneficial irrespective of complications on admission."}
{"id": "PMID:1089885", "title": "Filtration leukapheresis for granulocyte transfusion therapy. Clinical and laboratory studies.", "content": "To study the clinical efficacy of granulocytes obtained by filtration leukapheresis, patients with clinically evident infection and granulocyte counts of smaller than 500 per cubic millimeter were randomly assigned to receive conventional therapy alone or with a granulocyte transfusion obtained from a single donor each day for four days. Five of 19 control patients survived to day 20, and 15 of 17 in the transfused group survived. Comparison of the two populations for variables such as age, disease, and severity and type of infection revealed no other factor that could account for the difference in survival. Outcome was not demonstrated to be related to HL-A match, post-transfusion counts, or presence of leukocyte antibodies. Functional studies of granulocytes obtained by filtration leukapheresis showed only minor differences although appearance was altered. Granulocytes so obtained can be used safely and efficaciously as adjunctive therapy for infection associated with granulocytopenia.", "contents": "Filtration leukapheresis for granulocyte transfusion therapy. Clinical and laboratory studies. To study the clinical efficacy of granulocytes obtained by filtration leukapheresis, patients with clinically evident infection and granulocyte counts of smaller than 500 per cubic millimeter were randomly assigned to receive conventional therapy alone or with a granulocyte transfusion obtained from a single donor each day for four days. Five of 19 control patients survived to day 20, and 15 of 17 in the transfused group survived. Comparison of the two populations for variables such as age, disease, and severity and type of infection revealed no other factor that could account for the difference in survival. Outcome was not demonstrated to be related to HL-A match, post-transfusion counts, or presence of leukocyte antibodies. Functional studies of granulocytes obtained by filtration leukapheresis showed only minor differences although appearance was altered. Granulocytes so obtained can be used safely and efficaciously as adjunctive therapy for infection associated with granulocytopenia."}
{"id": "PMID:1089888", "title": "Immunopathogenesis of syphilitic glomerulonephritis. Elution of antitreponemal antibody from glomerular immune-complex deposits.", "content": "A renal biopsy was performed on a patient with syphilitic glomerulonephritis characterized clinically by the acute onset of nephrosis that responded dramatically to penicillin therapy. Histologic, electron microscopical and immunohistochemical studies revealed an early membranous glomerulonephritis characterized by the presence of subepithelial basement-membrane deposits containing IgG and C3. The glomerular lesion, however, differed from that in the usual case of membranous nephropathy in that distinctive segmental lesions were present at the tubular poles of the glomeruli, and all the glomeruli exhibited mild proliferation of mesangial cells in association with the presence of IgM within mesangial areas. Antibody elution studies performed on the renal-biopsy specimen demonstrated the presence of antitreponemal antibody within the glomerular immune-complex deposits. This finding indicates that the glomerular injury occasionally complicating secondary syphilis is due to the deposition of treponemal antigen-antitreponemal antibody complexes.", "contents": "Immunopathogenesis of syphilitic glomerulonephritis. Elution of antitreponemal antibody from glomerular immune-complex deposits. A renal biopsy was performed on a patient with syphilitic glomerulonephritis characterized clinically by the acute onset of nephrosis that responded dramatically to penicillin therapy. Histologic, electron microscopical and immunohistochemical studies revealed an early membranous glomerulonephritis characterized by the presence of subepithelial basement-membrane deposits containing IgG and C3. The glomerular lesion, however, differed from that in the usual case of membranous nephropathy in that distinctive segmental lesions were present at the tubular poles of the glomeruli, and all the glomeruli exhibited mild proliferation of mesangial cells in association with the presence of IgM within mesangial areas. Antibody elution studies performed on the renal-biopsy specimen demonstrated the presence of antitreponemal antibody within the glomerular immune-complex deposits. This finding indicates that the glomerular injury occasionally complicating secondary syphilis is due to the deposition of treponemal antigen-antitreponemal antibody complexes."}
{"id": "PMID:1089889", "title": "Clinical genetics: some neglected facets.", "content": "Like general medicine, clinical genetics has responsibilities to research, to dissemination and to service. Each exists at three broad levels. The elemental comprises acquisition of fact and development of technics. At the intermediate level coherence becomes a major concern: the discernment of relations among facts; the incorporation of data into ideas and insights; organization of clinical findings into a diagnosis; and the development of the rational management. The sophisticated level calls for theories and cosmologies at the scientific level, and cultivation of scholarship and of clinical wisdom. All nine compartments should be mutually correcting. If any of them is neglected or isolated from the rest, the whole will be impoverished-the student will suffocate in disconnected, empirical facts; fanciful theories will be spun from tenuous evidence; well established theory will be neglected by the practitioner; the best-intentioned schemes will have disastrous long-term consequences.", "contents": "Clinical genetics: some neglected facets. Like general medicine, clinical genetics has responsibilities to research, to dissemination and to service. Each exists at three broad levels. The elemental comprises acquisition of fact and development of technics. At the intermediate level coherence becomes a major concern: the discernment of relations among facts; the incorporation of data into ideas and insights; organization of clinical findings into a diagnosis; and the development of the rational management. The sophisticated level calls for theories and cosmologies at the scientific level, and cultivation of scholarship and of clinical wisdom. All nine compartments should be mutually correcting. If any of them is neglected or isolated from the rest, the whole will be impoverished-the student will suffocate in disconnected, empirical facts; fanciful theories will be spun from tenuous evidence; well established theory will be neglected by the practitioner; the best-intentioned schemes will have disastrous long-term consequences."}
{"id": "PMID:1089890", "title": "Atrioventricular block (first of two parts).", "content": "The development of His-bundle (H) electrocardiography and the concept of hemiblocks have prompted a re-examination of conventional thinking about the indications for treatment of atrioventricular (AV) block. The technic of treatment is not the issue; ventricular pacemaking is the only really effective and reliable method. The problem for this review is what these recent developments have contributed to deciding when and if a pacemaker should be installed.", "contents": "Atrioventricular block (first of two parts). The development of His-bundle (H) electrocardiography and the concept of hemiblocks have prompted a re-examination of conventional thinking about the indications for treatment of atrioventricular (AV) block. The technic of treatment is not the issue; ventricular pacemaking is the only really effective and reliable method. The problem for this review is what these recent developments have contributed to deciding when and if a pacemaker should be installed."}
{"id": "PMID:1089891", "title": "Reversible hydronephrosis in the neonate with urinary sepsis.", "content": "Hydroureteronephrosis without vesicoureteral reflux or lower-urinary-tract obstruction is uncommon in infants. There has been considerable interest in and controversy over the cause and management of this entity. We have cared for three neonates with severe hydroureteronephrosis after acute urinary tract infections, who were treated without operation.", "contents": "Reversible hydronephrosis in the neonate with urinary sepsis. Hydroureteronephrosis without vesicoureteral reflux or lower-urinary-tract obstruction is uncommon in infants. There has been considerable interest in and controversy over the cause and management of this entity. We have cared for three neonates with severe hydroureteronephrosis after acute urinary tract infections, who were treated without operation."}
{"id": "PMID:1089903", "title": "A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region.", "content": "Work reported here on the fungus Aspergillus nidulans has provided the first definitive demonstration of operon-type organisation in an eukaryote genome. It has been shown that the prnA and prnB genes concerned with proline metabolism form a gene cluster with the regulatory region lying between the two putative structural genes prnA and prnB. Regulatory mutations (prnd) probably leading to relief of carbon catabolite repression, map in between prnA and prnB and are cis-dominant with respect to both. The properties of these regulatory mutations and other findings suggest that carbon catabolite repression may be mediated by a negative control system in A. nidulans. This gene cluster is particularly interesting in view of its divergent orientation (with the regulatory region located in the centre of the operon) and for the fact that unlike the divergent operons known in prokaryotes, the divergent orientation is related to the way in which this particular operon may be regulated.", "contents": "A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region. Work reported here on the fungus Aspergillus nidulans has provided the first definitive demonstration of operon-type organisation in an eukaryote genome. It has been shown that the prnA and prnB genes concerned with proline metabolism form a gene cluster with the regulatory region lying between the two putative structural genes prnA and prnB. Regulatory mutations (prnd) probably leading to relief of carbon catabolite repression, map in between prnA and prnB and are cis-dominant with respect to both. The properties of these regulatory mutations and other findings suggest that carbon catabolite repression may be mediated by a negative control system in A. nidulans. This gene cluster is particularly interesting in view of its divergent orientation (with the regulatory region located in the centre of the operon) and for the fact that unlike the divergent operons known in prokaryotes, the divergent orientation is related to the way in which this particular operon may be regulated."}
{"id": "PMID:1089904", "title": "Initiator constitutive mutation with an 'up-promoter' effect in Aspergillus nidulans.", "content": "A mutation leading to strong constitutivity for a uric acid-xanthine permease in the lower eukaryote Aspergillus nidulans has been found to be tightly linked to the putative structural gene whose expression it controls in the cis configuration. In addition, it increases the maximal induced level of transport 2.5-fold. This is the first demonstration of an initiator or promoter mutation in an eukaryote.", "contents": "Initiator constitutive mutation with an 'up-promoter' effect in Aspergillus nidulans. A mutation leading to strong constitutivity for a uric acid-xanthine permease in the lower eukaryote Aspergillus nidulans has been found to be tightly linked to the putative structural gene whose expression it controls in the cis configuration. In addition, it increases the maximal induced level of transport 2.5-fold. This is the first demonstration of an initiator or promoter mutation in an eukaryote."}
{"id": "PMID:1089912", "title": "Cerebrospinal fluid cytology: practical clinical usefulness.", "content": "Sedimentation cytomorphology is a simple, inexpensive technique of collecting cells from cerebrospinal fluid and is of great clinical value. Important variables such as blood contamination, age of the patient, and general clincial condition must be considered. Specific conditions for which cerebrospinal fluid cytomorphology shows clinical usefulness include central nervous system leukemia, hemorrhage, immunopathies, infections, and brain tumors. Clinical examples of each of these conditions and their correlated cytograms are presented.", "contents": "Cerebrospinal fluid cytology: practical clinical usefulness. Sedimentation cytomorphology is a simple, inexpensive technique of collecting cells from cerebrospinal fluid and is of great clinical value. Important variables such as blood contamination, age of the patient, and general clincial condition must be considered. Specific conditions for which cerebrospinal fluid cytomorphology shows clinical usefulness include central nervous system leukemia, hemorrhage, immunopathies, infections, and brain tumors. Clinical examples of each of these conditions and their correlated cytograms are presented."}
{"id": "PMID:1089913", "title": "Serum clonazepam concentrations in children with absence seizures.", "content": "Clonazepam, a chlorinated derivative of nitrazepam, was administered to 10 children with absence seizures. Serum concentrations were measured after 8 weeks of treatment, at steady state. Seizure frequency reports and the 12-hour telemetered electroencephalogram were studied before and after 8 weeks of treatment to determine the frequency and duration of generalized spike-wave paroxysms. The clonazepam dosage ranged from 0.028 to 0.111 mg per kilogram and was reflected in serum levels ranging from 13 to 72 ng per milliliter, with an excellent correlation between dose and serum level. Eight of the 10 patients showed a significant decrease in seizure frequency, with three experiencing no seizures at all. Six patients had side effects, predominantly drowsiness and ataxia. This preliminary study shows clonazepam to be useful in the treatment of absence seizures in children and to merit further study.", "contents": "Serum clonazepam concentrations in children with absence seizures. Clonazepam, a chlorinated derivative of nitrazepam, was administered to 10 children with absence seizures. Serum concentrations were measured after 8 weeks of treatment, at steady state. Seizure frequency reports and the 12-hour telemetered electroencephalogram were studied before and after 8 weeks of treatment to determine the frequency and duration of generalized spike-wave paroxysms. The clonazepam dosage ranged from 0.028 to 0.111 mg per kilogram and was reflected in serum levels ranging from 13 to 72 ng per milliliter, with an excellent correlation between dose and serum level. Eight of the 10 patients showed a significant decrease in seizure frequency, with three experiencing no seizures at all. Six patients had side effects, predominantly drowsiness and ataxia. This preliminary study shows clonazepam to be useful in the treatment of absence seizures in children and to merit further study."}
{"id": "PMID:1089914", "title": "Abolition of electrically induced cortical seizures by stereotactic thalamic lesions. Evidence for descending thalamopontine medullar spinal connections in the centrencephalic epileptic system of the cat.", "content": "In experiments on the mechanism of epilepsy in normal adult cats, seizure patterns were established by electrical cortical and subcortical stimulation. Repeated cortical stimulation after bilateral coagulation of the lateral posterior thalamic nucleus resulted in a marked reduction in seizures, including complete abolishment of seizures in some animals. Histologic studies of the lateral posterior thalamic nucleus showed degeneration to the ventral anterior thalamic nucleus, the H zone of Forel, the mesencephalic-pontine reticular formations, the trigeminal motor nucleus, the lateral vestibular nucleus, and the intermediate horn of the entire spinal cord. This establishes a role for the lateral posterior thalamic nucleus in the centrencephalic system for all components of a typical epileptic attack.", "contents": "Abolition of electrically induced cortical seizures by stereotactic thalamic lesions. Evidence for descending thalamopontine medullar spinal connections in the centrencephalic epileptic system of the cat. In experiments on the mechanism of epilepsy in normal adult cats, seizure patterns were established by electrical cortical and subcortical stimulation. Repeated cortical stimulation after bilateral coagulation of the lateral posterior thalamic nucleus resulted in a marked reduction in seizures, including complete abolishment of seizures in some animals. Histologic studies of the lateral posterior thalamic nucleus showed degeneration to the ventral anterior thalamic nucleus, the H zone of Forel, the mesencephalic-pontine reticular formations, the trigeminal motor nucleus, the lateral vestibular nucleus, and the intermediate horn of the entire spinal cord. This establishes a role for the lateral posterior thalamic nucleus in the centrencephalic system for all components of a typical epileptic attack."}
{"id": "PMID:1089915", "title": "[Anesthesiological problems in renal transplantation].", "content": "The physiopathological changes observed in uraemia are briefly described and an account is given pharmacological considerations relevant to the induction and maintenance of anaesthesia and muscle relaxation, The anaesthesiological management of 20 kidneys transplants (17 from living donors and 3 from cadavers) is explained. Steps taken for intra-operative care and protection of the transplanted organ to aid its function recovery are explained.", "contents": "[Anesthesiological problems in renal transplantation]. The physiopathological changes observed in uraemia are briefly described and an account is given pharmacological considerations relevant to the induction and maintenance of anaesthesia and muscle relaxation, The anaesthesiological management of 20 kidneys transplants (17 from living donors and 3 from cadavers) is explained. Steps taken for intra-operative care and protection of the transplanted organ to aid its function recovery are explained."}
{"id": "PMID:1089919", "title": "[Hodgkin's disease in the malignant lymphoma category. Nosographic problems].", "content": "The classification of lymphoreticular tissue tumours is still confused. The tumours themselves take many forms and tissue reaction may be such as to preclude a clear distinction between neoplastic and inflammatory processes. This has led to what Willis called a chaos of definitions. The grouping proposed by Gall and Mallory in 1942 is still valid and includes Hodgkin's disease, lymphosarcoma, reticulum cell sarcoma, and follicular lymphoma. Its basis is both clinical and histological, particularly the latter; vary slight differences between the respective histological pictures and the possibility of transition forms. True leukaemias, however, must be excluded.", "contents": "[Hodgkin's disease in the malignant lymphoma category. Nosographic problems]. The classification of lymphoreticular tissue tumours is still confused. The tumours themselves take many forms and tissue reaction may be such as to preclude a clear distinction between neoplastic and inflammatory processes. This has led to what Willis called a chaos of definitions. The grouping proposed by Gall and Mallory in 1942 is still valid and includes Hodgkin's disease, lymphosarcoma, reticulum cell sarcoma, and follicular lymphoma. Its basis is both clinical and histological, particularly the latter; vary slight differences between the respective histological pictures and the possibility of transition forms. True leukaemias, however, must be excluded."}
{"id": "PMID:1089920", "title": "[Hodgkin's disease in the malignant lymphoma category. Relations between its histopathological and clinical aspects].", "content": "The need for active collaboration on the part of the histopathologist and the clinician in the management of hodgkin's disease is stressed, with particular reference to diagnosis, histological classification, and evaluation of the clinical stage.", "contents": "[Hodgkin's disease in the malignant lymphoma category. Relations between its histopathological and clinical aspects]. The need for active collaboration on the part of the histopathologist and the clinician in the management of hodgkin's disease is stressed, with particular reference to diagnosis, histological classification, and evaluation of the clinical stage."}
{"id": "PMID:1089921", "title": "[Current chemotherapeutic trends in the treatment of malignant lymphomas with special reference to Hodgkin's disease].", "content": "Sequential or simulatineous use is made of four types of antiproliferative drugs in the modern treatment of malignant lymphoma: alkylating drugs, including procarbazine; Vinca rosea alkaloids; antibiotics; corticosteroids. The simulataneous employment of two or more drugs appears to give best results, though it cannot eradicate the disease; this is possible with radiation or surgery in the early stages. Chemotherapy is nevertheless without alternative in the more advanced stages. Long periods of even complete remission may thus be achieved.", "contents": "[Current chemotherapeutic trends in the treatment of malignant lymphomas with special reference to Hodgkin's disease]. Sequential or simulatineous use is made of four types of antiproliferative drugs in the modern treatment of malignant lymphoma: alkylating drugs, including procarbazine; Vinca rosea alkaloids; antibiotics; corticosteroids. The simulataneous employment of two or more drugs appears to give best results, though it cannot eradicate the disease; this is possible with radiation or surgery in the early stages. Chemotherapy is nevertheless without alternative in the more advanced stages. Long periods of even complete remission may thus be achieved."}
{"id": "PMID:1089922", "title": "[On a case of exlusively splenic Hodgkin's disease, with histological detection alone].", "content": "A case of Hodgkin's disease confined to the spleen is described. A picture of sepsis with rapidly progessive cachexia was present from the outset. Necrosy revealed no gross evidence of the disease and diagnosis was solely dependent on the histological evidence.", "contents": "[On a case of exlusively splenic Hodgkin's disease, with histological detection alone]. A case of Hodgkin's disease confined to the spleen is described. A picture of sepsis with rapidly progessive cachexia was present from the outset. Necrosy revealed no gross evidence of the disease and diagnosis was solely dependent on the histological evidence."}
{"id": "PMID:1089927", "title": "Gonadotropin responses following luteinizing hormone releasing hormone administration in normal subjects.", "content": "Synthetic luteinizing hormone releasing hormone (LHRH), 100 mug, was administered intravenously to 29 normal individuals. In male subjects, both baseline and peak LH response values showed minimal variations between subjects. The FSH baseline and response were more variable. In normal females the FSH and LH baseline values and response to LHRH infusion showed greater variation than that seen in male subjects, and this variation correlated with the phase of the menstrual cycle. In perimenopausal women with elevated baseline gonadotropins, the absolute LH peak values were comparable to those seen during the periovulatory period of the menstrual cycle, while FSH baseline and peak values were unmistakably higher than those observed in the normally menstruating women. Three women under gonadotropic suppression by three different drugs were investigated; megestrol suppressed the LHRH response more effectively than either diethylstilbestrol or an oral contraceptive. The LHRH stimulation test reflects only the ability of the pituitary cell to release gonadotropins. In a normal individual, however, some justifiable assumptions can be made regarding the immediate past synthesis and release versus accumulation of pituitary LH.", "contents": "Gonadotropin responses following luteinizing hormone releasing hormone administration in normal subjects. Synthetic luteinizing hormone releasing hormone (LHRH), 100 mug, was administered intravenously to 29 normal individuals. In male subjects, both baseline and peak LH response values showed minimal variations between subjects. The FSH baseline and response were more variable. In normal females the FSH and LH baseline values and response to LHRH infusion showed greater variation than that seen in male subjects, and this variation correlated with the phase of the menstrual cycle. In perimenopausal women with elevated baseline gonadotropins, the absolute LH peak values were comparable to those seen during the periovulatory period of the menstrual cycle, while FSH baseline and peak values were unmistakably higher than those observed in the normally menstruating women. Three women under gonadotropic suppression by three different drugs were investigated; megestrol suppressed the LHRH response more effectively than either diethylstilbestrol or an oral contraceptive. The LHRH stimulation test reflects only the ability of the pituitary cell to release gonadotropins. In a normal individual, however, some justifiable assumptions can be made regarding the immediate past synthesis and release versus accumulation of pituitary LH."}
{"id": "PMID:1089928", "title": "Diagnostic use of luteinizing hormone releasing hormone in primary amenorrhea.", "content": "Thirty-eight patients with primary amenorrhea were evaluated by the administration of luteinizing hormone releasing hormone (LHRH). The LHRH stimulation test was not helpful in differentiating those patients who will proceed to normal menses from those who will continue to have prolonged dysfunction. Similar responses were observed in patients with different pathology, which indicates that the LHRH test must be utilized in conjunction with a carefully planned evaluation if it is to be useful in differentiating a hypothalamic from a pituitary defect. The finding of a patient with hypergonadotropism by serum radioimmunoassay and negative gonadotropins by bioassay suggests that the radioimmunoassay may detect a biologically inactive molecule and indicates that both assays are essential in the evaluation of primary amenorrhea.", "contents": "Diagnostic use of luteinizing hormone releasing hormone in primary amenorrhea. Thirty-eight patients with primary amenorrhea were evaluated by the administration of luteinizing hormone releasing hormone (LHRH). The LHRH stimulation test was not helpful in differentiating those patients who will proceed to normal menses from those who will continue to have prolonged dysfunction. Similar responses were observed in patients with different pathology, which indicates that the LHRH test must be utilized in conjunction with a carefully planned evaluation if it is to be useful in differentiating a hypothalamic from a pituitary defect. The finding of a patient with hypergonadotropism by serum radioimmunoassay and negative gonadotropins by bioassay suggests that the radioimmunoassay may detect a biologically inactive molecule and indicates that both assays are essential in the evaluation of primary amenorrhea."}
{"id": "PMID:1089929", "title": "Gonadotropin response to luteinizing hormone releasing hormone administration in secondary amenorrhea and galactorrhea syndromes.", "content": "Luteinizing hormone releasing hormone (LHRH), 100 mug, was administered to 60 patients with secondary amenorrhea. No side effects or adverse reactions occurred. Delayed FSH and LH peak outputs were observed in patients receiving LHRH subcutaneously rather than intravenously, but responses were otherwise comparable. The majority of patients with secondary amenorrhea had responses which fell into the 95% confidence limits of responses for normal ovulating women. Responses of patients with different forms of dysfunction may be similar, and patients with similar pathology may respond quite differently. The LHRH stimulation test may assist in the diagnosis of particular hypothalamic, pituitary, or gonadal dysfunction, but it must be used clinically in the context of a carefully integrated study.", "contents": "Gonadotropin response to luteinizing hormone releasing hormone administration in secondary amenorrhea and galactorrhea syndromes. Luteinizing hormone releasing hormone (LHRH), 100 mug, was administered to 60 patients with secondary amenorrhea. No side effects or adverse reactions occurred. Delayed FSH and LH peak outputs were observed in patients receiving LHRH subcutaneously rather than intravenously, but responses were otherwise comparable. The majority of patients with secondary amenorrhea had responses which fell into the 95% confidence limits of responses for normal ovulating women. Responses of patients with different forms of dysfunction may be similar, and patients with similar pathology may respond quite differently. The LHRH stimulation test may assist in the diagnosis of particular hypothalamic, pituitary, or gonadal dysfunction, but it must be used clinically in the context of a carefully integrated study."}
{"id": "PMID:1089930", "title": "Pituitary response to a new analog of luteinizing hormone releasing factor during the menstrual cycle.", "content": "A new analog of luteinizing-hormone-releasing factor, (Des-Gly-NH-2-10, Pro-ethylamide-9)-LHRF, was tested in healthy women with regular menstrual cycles. This synthetic nonapeptide amide was injected intravenously in a dose of 200 mug into 15 normal female volunteers in the follicular, preovulatory, and luteal phases of the menstrual cycle, and the serum LH and FSH responses were compared with those to the same dose of the synthetic decapeptide LHRF in 15 controls. The new analog exhibited significantly higher activity than the synthetic decapeptide LHRF in the release of LH in the follicular (P smaller than 0.05), preovulatory (P smaller than 0.01), and luteal phases (P smaller than 0.05). In contrast, no statistical significance in the magnitude of FSH response was demonstrated in any phase of the menstrual cycle. The serum LH and FSH response to the nonapeptide amide suggested a longer physiologic half-life of the new LHRF analog. The results are similar to a previous report in men with normal gonadal function, and a clinical usefulness for the new analog is expected.", "contents": "Pituitary response to a new analog of luteinizing hormone releasing factor during the menstrual cycle. A new analog of luteinizing-hormone-releasing factor, (Des-Gly-NH-2-10, Pro-ethylamide-9)-LHRF, was tested in healthy women with regular menstrual cycles. This synthetic nonapeptide amide was injected intravenously in a dose of 200 mug into 15 normal female volunteers in the follicular, preovulatory, and luteal phases of the menstrual cycle, and the serum LH and FSH responses were compared with those to the same dose of the synthetic decapeptide LHRF in 15 controls. The new analog exhibited significantly higher activity than the synthetic decapeptide LHRF in the release of LH in the follicular (P smaller than 0.05), preovulatory (P smaller than 0.01), and luteal phases (P smaller than 0.05). In contrast, no statistical significance in the magnitude of FSH response was demonstrated in any phase of the menstrual cycle. The serum LH and FSH response to the nonapeptide amide suggested a longer physiologic half-life of the new LHRF analog. The results are similar to a previous report in men with normal gonadal function, and a clinical usefulness for the new analog is expected."}
{"id": "PMID:1089932", "title": "Proplast in dental facial reconstruction.", "content": "Successful clinical applications since early 1970 have occurred with the use of temporomandibular condylar prosthesis and endosseous blade-vent implants coated with porous proplast. Proplast as a bulk material has also been used to augment atropic mandibular alveolar ridges and deficient facial contours in the mental, mandibular border, and zygomatic areas.", "contents": "Proplast in dental facial reconstruction. Successful clinical applications since early 1970 have occurred with the use of temporomandibular condylar prosthesis and endosseous blade-vent implants coated with porous proplast. Proplast as a bulk material has also been used to augment atropic mandibular alveolar ridges and deficient facial contours in the mental, mandibular border, and zygomatic areas."}
{"id": "PMID:1089951", "title": "Effect of various lysosomes and endotoxin on vascular permeability in frogs and mice.", "content": "Blood-lymph permeability increasing effects of frog liver lysosomes, Escherichia coli 0111 endotoxin, bradykinin and serotonin were demonstrated in frogs with a method developed by the authors. These actions were expressed in a faster dye saturation in the lymph as compared to that of the controls. 2. The method is based on the determinations of concentration of Evans blue transported as protein-bound dye into the lymph. 3. Frog liver and polymorphonuclear leukocyte lysosomes had a capillary permeability increasing action tested by local skin response when injecting Evans blue intravenously in mice. 4. All these phenomena are similar to events described earlier in mammalian systems.", "contents": "Effect of various lysosomes and endotoxin on vascular permeability in frogs and mice. Blood-lymph permeability increasing effects of frog liver lysosomes, Escherichia coli 0111 endotoxin, bradykinin and serotonin were demonstrated in frogs with a method developed by the authors. These actions were expressed in a faster dye saturation in the lymph as compared to that of the controls. 2. The method is based on the determinations of concentration of Evans blue transported as protein-bound dye into the lymph. 3. Frog liver and polymorphonuclear leukocyte lysosomes had a capillary permeability increasing action tested by local skin response when injecting Evans blue intravenously in mice. 4. All these phenomena are similar to events described earlier in mammalian systems."}
{"id": "PMID:1089949", "title": "[The state of knowledge of the class of trematodes and the chief problems in studying them].", "content": "The paper deals with the current state of knowledge of Trematoda, trends and aims of further investigations. Up to now the classification system of trematodes has been unsatisfactory though for several large groups it is elaborated with due regard to the biology and ecology of these worms. The main deficiency of the present-day taxonomy of trematodes consists in the existence of two separate classifications, for adult worms and for their larvae (cercariae). Among the existing systems the classification suggested by Odening (1960-1963) is of the greatest interest. We do not agree with his division of the class into three subclasses (including Aspidogastrea) and Diginea into two subclasses, \"Sporocystoinei\" and \"Redioinei\", but follow the composition of orders established by him. Great difficulties arise in establishing the characters, taxonomic criteria of trematodes. However, a number of characters can be suggested for differentiation of species and genera at various developmental stages. Deficiency in the taxonomy of these worms is due to a wrong evaluation of their characters and to the lack of common collection and treatment techniques. On describing species one should proceed, if possible, from the whole complex of morphological and biological peculiarities of different ontogenetic stages of the forms under study. A study of the ultrafine structure, hysto- and biochemistry of trematodes will enable us to associate better the morphology and the function of organs and to go deeper into the functional morphology and functional biochemistry. The main task of further investigations is closely connected with the elaboration of a natural system of Trematoda, a study of diverse and deep interrelations in the \"parasite-host\" system and with the development of scientific methods of control of pathogenic species on the biological basis.", "contents": "[The state of knowledge of the class of trematodes and the chief problems in studying them]. The paper deals with the current state of knowledge of Trematoda, trends and aims of further investigations. Up to now the classification system of trematodes has been unsatisfactory though for several large groups it is elaborated with due regard to the biology and ecology of these worms. The main deficiency of the present-day taxonomy of trematodes consists in the existence of two separate classifications, for adult worms and for their larvae (cercariae). Among the existing systems the classification suggested by Odening (1960-1963) is of the greatest interest. We do not agree with his division of the class into three subclasses (including Aspidogastrea) and Diginea into two subclasses, \"Sporocystoinei\" and \"Redioinei\", but follow the composition of orders established by him. Great difficulties arise in establishing the characters, taxonomic criteria of trematodes. However, a number of characters can be suggested for differentiation of species and genera at various developmental stages. Deficiency in the taxonomy of these worms is due to a wrong evaluation of their characters and to the lack of common collection and treatment techniques. On describing species one should proceed, if possible, from the whole complex of morphological and biological peculiarities of different ontogenetic stages of the forms under study. A study of the ultrafine structure, hysto- and biochemistry of trematodes will enable us to associate better the morphology and the function of organs and to go deeper into the functional morphology and functional biochemistry. The main task of further investigations is closely connected with the elaboration of a natural system of Trematoda, a study of diverse and deep interrelations in the \"parasite-host\" system and with the development of scientific methods of control of pathogenic species on the biological basis."}
{"id": "PMID:1089974", "title": "Oral-pharyngeal development and evaluation.", "content": "The recognition and evaluation of deficits and abnormalities related to the development of feeding require comprehensive and systematic observation. This article describes both gross and fine motor coordination, as well as primitive reflexive movement, which influence eating. Oral-pharyngeal reflexes, the function of the tongue, oral hygiene, and the condition of the teeth are described relative to their influence on the feeding process. The development of eating behaviors of children are briefly outlined.", "contents": "Oral-pharyngeal development and evaluation. The recognition and evaluation of deficits and abnormalities related to the development of feeding require comprehensive and systematic observation. This article describes both gross and fine motor coordination, as well as primitive reflexive movement, which influence eating. Oral-pharyngeal reflexes, the function of the tongue, oral hygiene, and the condition of the teeth are described relative to their influence on the feeding process. The development of eating behaviors of children are briefly outlined."}
{"id": "PMID:1089975", "title": "Legg-Calv\u00e9-Perthes disease.", "content": "The incidence, pathology, etiology, clinical characteristics, differential diagnosis, and treatment of Legg-Calv\u00e9-Perthes disease are reviewed. Physical therapy and follow-up care are discussed, with emphasis on the relationship between orthopedic and physical therapy services.", "contents": "Legg-Calv\u00e9-Perthes disease. The incidence, pathology, etiology, clinical characteristics, differential diagnosis, and treatment of Legg-Calv\u00e9-Perthes disease are reviewed. Physical therapy and follow-up care are discussed, with emphasis on the relationship between orthopedic and physical therapy services."}
{"id": "PMID:1089978", "title": "The current use of prophylactic antibiotics in plastic and reconstructive surgery.", "content": "A questionnaire distributed to plastic surgeons to determine their patterns of usage of systemic prophylactic antibacterial agents was answered by 1,025 respondents (73.7 percent). The patterns of usage among the respondents were determined for 29 circumstances and operations for the time of administration, and as influenced by 11 modifying factors. The data present the patterns of antibacterial use by plastic surgeons at this time.", "contents": "The current use of prophylactic antibiotics in plastic and reconstructive surgery. A questionnaire distributed to plastic surgeons to determine their patterns of usage of systemic prophylactic antibacterial agents was answered by 1,025 respondents (73.7 percent). The patterns of usage among the respondents were determined for 29 circumstances and operations for the time of administration, and as influenced by 11 modifying factors. The data present the patterns of antibacterial use by plastic surgeons at this time."}
{"id": "PMID:1089981", "title": "Surgical problems in the excision and repair of radiated tissue.", "content": "Surgical procedures for tissues damaged by radiation are fraught with the possibility of multiple and serious complications. A knowledge of the altered pathophysiology of such tissues (chiefly diminished blood supply, fibrosis, and direct cellular reparative potential) is necessary for the surgeon to make a proper judgment. Recognition of the pathognomic signs in the skin is important. The possibility of malignant change must be kept in mind. Surgical treatment must include the excision of radiated tissues back to good blood supply, and then proper and adequate coverage by the simplest and best means. If the surrounding local tissues are so altered that they are unsuitable for coverage, structures from a distance must be used. Prior planning of the coverage before the excision pays dividends by preventing disastrous complications.", "contents": "Surgical problems in the excision and repair of radiated tissue. Surgical procedures for tissues damaged by radiation are fraught with the possibility of multiple and serious complications. A knowledge of the altered pathophysiology of such tissues (chiefly diminished blood supply, fibrosis, and direct cellular reparative potential) is necessary for the surgeon to make a proper judgment. Recognition of the pathognomic signs in the skin is important. The possibility of malignant change must be kept in mind. Surgical treatment must include the excision of radiated tissues back to good blood supply, and then proper and adequate coverage by the simplest and best means. If the surrounding local tissues are so altered that they are unsuitable for coverage, structures from a distance must be used. Prior planning of the coverage before the excision pays dividends by preventing disastrous complications."}
{"id": "PMID:1089983", "title": "Skin graft survival on avascular defects.", "content": "Full-thickness skin grafts placed on bare rabbit ear cartilage were revascularized, whereas split-skin grafts on the same kinds of areas failed. The contrast is most likely due to differences in the skin graft thickness and the vascular patterns in the grafts. Full-thickness rat skin grafts placed over the same sized underlying silicone sheet implants did not survive. When the graft was made 3 mm larger on all sides than the implant, all or a portion of the graft over the implant survived. Quantitation of the area of graft survival is presented. Prepared grafts (replaced on their donor areas for 48 hours) on prepared beds and fresh grafts on prepared beds had slightly larger areas of graft surviving over an avascular defect. On the basis of this investigation, we suggest that a full-thickness skin graft placed on a prepared peripheral bed may make possible the greatest area of bridging over an avascular defect.", "contents": "Skin graft survival on avascular defects. Full-thickness skin grafts placed on bare rabbit ear cartilage were revascularized, whereas split-skin grafts on the same kinds of areas failed. The contrast is most likely due to differences in the skin graft thickness and the vascular patterns in the grafts. Full-thickness rat skin grafts placed over the same sized underlying silicone sheet implants did not survive. When the graft was made 3 mm larger on all sides than the implant, all or a portion of the graft over the implant survived. Quantitation of the area of graft survival is presented. Prepared grafts (replaced on their donor areas for 48 hours) on prepared beds and fresh grafts on prepared beds had slightly larger areas of graft surviving over an avascular defect. On the basis of this investigation, we suggest that a full-thickness skin graft placed on a prepared peripheral bed may make possible the greatest area of bridging over an avascular defect."}
{"id": "PMID:1089984", "title": "Marked prolongation of skin homograft survival with niridazole. A first report.", "content": "Niridazole, a nitroheterocyclic drug used to treat helminth infections is reported for the first time to suppress cell-mediated immunity, as ascertained by multiple in vivo assays. Skin homograft survival time was significantly prolonged in mice, without loss of animals due to drug toxicity.", "contents": "Marked prolongation of skin homograft survival with niridazole. A first report. Niridazole, a nitroheterocyclic drug used to treat helminth infections is reported for the first time to suppress cell-mediated immunity, as ascertained by multiple in vivo assays. Skin homograft survival time was significantly prolonged in mice, without loss of animals due to drug toxicity."}
{"id": "PMID:1089997", "title": "Stereology: methods for quantitative light and electron microscopy.", "content": "There are available to the biologist sophisticated methods for the quantitative determination of many structural parameters of tissues; the methds are covered by the term stereology and are based on statistical sampling techniques introduced by geologists and materials scientists. Methods for determining the volume fractions of various compartments within a cell or tissue are presented, together with methods for determination of surface area, surface-to-volume ratio, and particle size and size distribution. The problems involved in applying these methods to tissue sections are discussed, together with the problems arising from anisotropy within the tissues. Sampling methods and test systems in common use are described and discussed in relation to available counting and recording systems, and the potentialities of automatic image analysing equipment in this field are assessed. A discussion of shape recognition follows, with a survey of recent stereological work.", "contents": "Stereology: methods for quantitative light and electron microscopy. There are available to the biologist sophisticated methods for the quantitative determination of many structural parameters of tissues; the methds are covered by the term stereology and are based on statistical sampling techniques introduced by geologists and materials scientists. Methods for determining the volume fractions of various compartments within a cell or tissue are presented, together with methods for determination of surface area, surface-to-volume ratio, and particle size and size distribution. The problems involved in applying these methods to tissue sections are discussed, together with the problems arising from anisotropy within the tissues. Sampling methods and test systems in common use are described and discussed in relation to available counting and recording systems, and the potentialities of automatic image analysing equipment in this field are assessed. A discussion of shape recognition follows, with a survey of recent stereological work."}
{"id": "PMID:1089993", "title": "Echocardiography in valvular heart disease.", "content": "Echocardiography is a useful new technique that allows for the diagnosis and assessment of the severity of various forms of valvular heart disease. It is a safe and noninvasive procedure that can readily be used on the critically ill as well as the ambulatory patient. Since the examination can be easily repeated, echocardiography can be used to study a patient over an extended period of time to follow the severity of the disease. With proper care and experience in the performance and interpretation, the cardiologist can derive much useful information to aid in the initial evaluation and long-term follow-up of patients with various forms of valvular disease. With improvements in instrumentation and the use of newer techniques, the usefulness of ultrasound will be further enhanced.", "contents": "Echocardiography in valvular heart disease. Echocardiography is a useful new technique that allows for the diagnosis and assessment of the severity of various forms of valvular heart disease. It is a safe and noninvasive procedure that can readily be used on the critically ill as well as the ambulatory patient. Since the examination can be easily repeated, echocardiography can be used to study a patient over an extended period of time to follow the severity of the disease. With proper care and experience in the performance and interpretation, the cardiologist can derive much useful information to aid in the initial evaluation and long-term follow-up of patients with various forms of valvular disease. With improvements in instrumentation and the use of newer techniques, the usefulness of ultrasound will be further enhanced."}
{"id": "PMID:1089998", "title": "The perception of speech signals.", "content": "A brief outline of the mechanism of speech production is given. It is shown that the motion of the articulators is encoded in changes in the spectra of speech waveforms. Parameters derived from these spectra form the basis of a method of speech synthesis. The perception of speech is seen as a decoding of the acoustic features of the speech wave. The organs of the hearing system which are involved in this process are briefly described. An attempt is made to relate the results of auditory psychophysics to the physiology of the hearing mechanism. The acoustic features which are relevant for the perception of the various classes of speech sounds are described. These have mostly been obtained by experiments involving synthesized speech. Finally the results of some experiments in dichotic listening are presented which emphasize the differences in the perception of speech and other sounds.", "contents": "The perception of speech signals. A brief outline of the mechanism of speech production is given. It is shown that the motion of the articulators is encoded in changes in the spectra of speech waveforms. Parameters derived from these spectra form the basis of a method of speech synthesis. The perception of speech is seen as a decoding of the acoustic features of the speech wave. The organs of the hearing system which are involved in this process are briefly described. An attempt is made to relate the results of auditory psychophysics to the physiology of the hearing mechanism. The acoustic features which are relevant for the perception of the various classes of speech sounds are described. These have mostly been obtained by experiments involving synthesized speech. Finally the results of some experiments in dichotic listening are presented which emphasize the differences in the perception of speech and other sounds."}
{"id": "PMID:1089999", "title": "Glucagon: role in the hyperglycemia of diabetes mellitus.", "content": "Glucagon suppression by somatostatin reduces or abolishes hyperglycemia in dogs made insulin-deficient by somatostatin, alloxan, or total pancreatectomy. This suggests that the development of severe diabetic hyperglycemia requires the presence of glucagon, whether secreted by pancreatic or newly identified gastrointestinal A cells, as well as a lack of insulin. Glucagon suppression could improve therapeutic glucoregulation in diabetes.", "contents": "Glucagon: role in the hyperglycemia of diabetes mellitus. Glucagon suppression by somatostatin reduces or abolishes hyperglycemia in dogs made insulin-deficient by somatostatin, alloxan, or total pancreatectomy. This suggests that the development of severe diabetic hyperglycemia requires the presence of glucagon, whether secreted by pancreatic or newly identified gastrointestinal A cells, as well as a lack of insulin. Glucagon suppression could improve therapeutic glucoregulation in diabetes."}
{"id": "PMID:1090001", "title": "Ventral medial hypothalamus: involvement in hypoglycemic convulsions.", "content": "After the ventral medial hypothalamus of mice was lesioned with gold thioglucose, the dose of insulin required to produce convulsions in 50 percent of the animals was doubled compared to that in nonlesionad controls. No dose of insulin, up to 50 milliunits per gram, produced convulsions in more than 60 percent of the lesioned mice, even though blood glucose levels fell to approximately 24 milligram percent.", "contents": "Ventral medial hypothalamus: involvement in hypoglycemic convulsions. After the ventral medial hypothalamus of mice was lesioned with gold thioglucose, the dose of insulin required to produce convulsions in 50 percent of the animals was doubled compared to that in nonlesionad controls. No dose of insulin, up to 50 milliunits per gram, produced convulsions in more than 60 percent of the lesioned mice, even though blood glucose levels fell to approximately 24 milligram percent."}
{"id": "PMID:1090006", "title": "Yeast chromatin subunit structure.", "content": "Micrococcal nuclease digestion of in situ (intranuclear) and in vitro yeast chromatin produces distributions of DNA molecules of discrete sizes. In both cases, these molecules appear to be integral multiples of the smallest size on polyacrylamide gel electrophoresis. This result implies a widespread generic occurrence of the periodic organization of chromatin seen in mammalian systems.", "contents": "Yeast chromatin subunit structure. Micrococcal nuclease digestion of in situ (intranuclear) and in vitro yeast chromatin produces distributions of DNA molecules of discrete sizes. In both cases, these molecules appear to be integral multiples of the smallest size on polyacrylamide gel electrophoresis. This result implies a widespread generic occurrence of the periodic organization of chromatin seen in mammalian systems."}
{"id": "PMID:1090007", "title": "The problem of the origin of fibroblasts in the postnatal ontogenesis of mammals.", "content": "An analysis is made of the author's data and the literature information on the origin and patterns of differentiation of the fibroblasts of connective tissue during the postnatal ontogenesis of mammals. The hypothesis of the existence of two types of collagen-producing cells (fibroblasts) - protective-trophic (short-lived population) and supporting (long-lived population) - is substantiated. Hypotheses are advanced on the biological role of these types of fibroblasts and their origin in phylogenesis.", "contents": "The problem of the origin of fibroblasts in the postnatal ontogenesis of mammals. An analysis is made of the author's data and the literature information on the origin and patterns of differentiation of the fibroblasts of connective tissue during the postnatal ontogenesis of mammals. The hypothesis of the existence of two types of collagen-producing cells (fibroblasts) - protective-trophic (short-lived population) and supporting (long-lived population) - is substantiated. Hypotheses are advanced on the biological role of these types of fibroblasts and their origin in phylogenesis."}
{"id": "PMID:1090008", "title": "The possibility of using cytogenetic methods in the investigation of the origin of fibroblast-like cells of a focus of inflammation.", "content": "An improved method of study of chromosome preparations of fibroblast-like cells of a focus of inflammation in mammals is described. The essence of the method consists of a supplementary brief culturing in vitro of cells of a focus of inflammation at its proliferative phase.", "contents": "The possibility of using cytogenetic methods in the investigation of the origin of fibroblast-like cells of a focus of inflammation. An improved method of study of chromosome preparations of fibroblast-like cells of a focus of inflammation in mammals is described. The essence of the method consists of a supplementary brief culturing in vitro of cells of a focus of inflammation at its proliferative phase."}
{"id": "PMID:1090010", "title": "History of mental health services in South Africa. Part VI. The Transvaal.", "content": "Weskoppies Hospital began life in 1892 under the South African Republic. The clinical head of this hospital is now the Professor of Psychiatry of Pretoria University, and it has close links with the H. F. Verwoerd Hospital. Sterkfontein Hospital was opened in 1943, and admits most of its patients from Johannesburg. It has now been placed under the clinical control of the Professor of Psychiatry of the University of the Witwatersrand, and is thus linked with the psychiatric departments of the Johannesburg General and Tara Hospitals.", "contents": "History of mental health services in South Africa. Part VI. The Transvaal. Weskoppies Hospital began life in 1892 under the South African Republic. The clinical head of this hospital is now the Professor of Psychiatry of Pretoria University, and it has close links with the H. F. Verwoerd Hospital. Sterkfontein Hospital was opened in 1943, and admits most of its patients from Johannesburg. It has now been placed under the clinical control of the Professor of Psychiatry of the University of the Witwatersrand, and is thus linked with the psychiatric departments of the Johannesburg General and Tara Hospitals."}
{"id": "PMID:1090013", "title": "A therapeutic response to a single diagnostic dose of luteinising hormone-releasing hormone.", "content": "Luteinising hormone-releasing hormone (LH-RH) administration is a useful provocative test for the evaluation of the hypothalamic-pituitary-gonadal axis. Seven cases with oligomenorrhoea or amenorrhoea are reported, in which a single diagnostic injection of LH-RH produced an apparent therapeutic response. Six patients converted to a regular normal menstrual cycle, and 4 of these had evidence of ovulation. The seventh patient conceived. It is postulated that in some cases of hypothalamic menstrual dysfunction the gonadotrophic imbalance may be corrected by a single intravenous dose of LH-RH.", "contents": "A therapeutic response to a single diagnostic dose of luteinising hormone-releasing hormone. Luteinising hormone-releasing hormone (LH-RH) administration is a useful provocative test for the evaluation of the hypothalamic-pituitary-gonadal axis. Seven cases with oligomenorrhoea or amenorrhoea are reported, in which a single diagnostic injection of LH-RH produced an apparent therapeutic response. Six patients converted to a regular normal menstrual cycle, and 4 of these had evidence of ovulation. The seventh patient conceived. It is postulated that in some cases of hypothalamic menstrual dysfunction the gonadotrophic imbalance may be corrected by a single intravenous dose of LH-RH."}
{"id": "PMID:1090014", "title": "Influence of tetrahydro-aminacrine on muscle pains after suxamethonium.", "content": "The incidence and severity of muscle pains were investigated in patients who had been given suxamethonium and thiopentone anesthesia for bronchoscopy. Group I patients received no other drugs. Groups II and III received d-tubocurarine and THA respectively before suxamethonium. Administration of these drugs resulted in a highly significant reduction in the incidence of severe and moderate pains. The use of d-tubocurarine necessitated a significantly greater amount of suxamethonium to maintain paralysis.", "contents": "Influence of tetrahydro-aminacrine on muscle pains after suxamethonium. The incidence and severity of muscle pains were investigated in patients who had been given suxamethonium and thiopentone anesthesia for bronchoscopy. Group I patients received no other drugs. Groups II and III received d-tubocurarine and THA respectively before suxamethonium. Administration of these drugs resulted in a highly significant reduction in the incidence of severe and moderate pains. The use of d-tubocurarine necessitated a significantly greater amount of suxamethonium to maintain paralysis."}
{"id": "PMID:1090015", "title": "The present status of heart transplantation.", "content": "Heart transplantation still remains the only form of treatment for patients suffering from terminal heart failure as the result of diffuse myocardial disease. The lack of enthusiasm for this operation has no scientific or moral basis. Our own limited experience and the much wider experience of other workers in this field show that with proper patient and donor selection and careful postoperative management, the results of this operation compare very favourably with those of any transplantation of other organs and in the surgical treatment of other types of terminal diseases.", "contents": "The present status of heart transplantation. Heart transplantation still remains the only form of treatment for patients suffering from terminal heart failure as the result of diffuse myocardial disease. The lack of enthusiasm for this operation has no scientific or moral basis. Our own limited experience and the much wider experience of other workers in this field show that with proper patient and donor selection and careful postoperative management, the results of this operation compare very favourably with those of any transplantation of other organs and in the surgical treatment of other types of terminal diseases."}
{"id": "PMID:1090016", "title": "Left ventricular bypass.", "content": "The removal of the patient's own diseased heart in order to perform a total cardiac transplantation has several disadvantages. A new technique for bypassing the patient's diseased left ventricle by using a cardiac allograft has been developed in our laboratory and applied clinically in 2 patients. This technique carried no direct surgical mortality, and no complications resulting from the patient's own heart being left in situ have been observed thus far. The presence of a second heart lying partly in the anterior mediastinum and partly in the right pleural space appeared to have no deleterious effects. Several advantages over the conventional transplant have been noted and are discussed.", "contents": "Left ventricular bypass. The removal of the patient's own diseased heart in order to perform a total cardiac transplantation has several disadvantages. A new technique for bypassing the patient's diseased left ventricle by using a cardiac allograft has been developed in our laboratory and applied clinically in 2 patients. This technique carried no direct surgical mortality, and no complications resulting from the patient's own heart being left in situ have been observed thus far. The presence of a second heart lying partly in the anterior mediastinum and partly in the right pleural space appeared to have no deleterious effects. Several advantages over the conventional transplant have been noted and are discussed."}
{"id": "PMID:1090017", "title": "History of the mental health services in South Africa. Part V. Natal.", "content": "The early history of psychiatry in Natal is the history of Town Hill Hospital. A second hospital was opened at Fort Napier in 1927. They have, for clinical purposes, now been placed under the control of the Professor of Psychiatry at the medical school of Natal University, and it is planned to merge them into a single hospital.", "contents": "History of the mental health services in South Africa. Part V. Natal. The early history of psychiatry in Natal is the history of Town Hill Hospital. A second hospital was opened at Fort Napier in 1927. They have, for clinical purposes, now been placed under the control of the Professor of Psychiatry at the medical school of Natal University, and it is planned to merge them into a single hospital."}
{"id": "PMID:1090018", "title": "Septic complications following endoscopic retrograde cholangiopancreatography.", "content": "In four patients, septic complications developed following endoscopic retrograde cholangiopancreatography which required surgical intervention. The pathogenesis involves stasis in the pancreatic or biliary tree, but the source of infection is unclear. Prompt recognition and early surgical intervention should decrease the seriousness of these complications.", "contents": "Septic complications following endoscopic retrograde cholangiopancreatography. In four patients, septic complications developed following endoscopic retrograde cholangiopancreatography which required surgical intervention. The pathogenesis involves stasis in the pancreatic or biliary tree, but the source of infection is unclear. Prompt recognition and early surgical intervention should decrease the seriousness of these complications."}
{"id": "PMID:1090019", "title": "Cholecystokinin cholangiography and analysis of duodenal bile in the investigation of pain in the right upper quadrant of the abdomen without gallstones.", "content": "Thirty-one patients with recurrent symptoms of the biliary tract and repeated normal oral cholecystograms were studied by a combination of cholecystokinin cholangiography and biliary drainage. Ten patients had reduplication of their symptons because of dyskinetic contractions or obstruction of the cystic duct, and seven patients had delayed gallbladder emptying without pain due to hypokinetic contractions. Five patients had abnormal duodenal bile characterized by supersaturation and the presence of crystals or bacteria. Based upon these studies, 22 patients had cholecystectomy and 20 were cured, while two showed improvement. There were no therapeutic failures. Cholecystokinin cholangiography capably detects the presence of neuromuscular disease of the gallbladder wall, whereas the oral cholecystogram tests for mucosal function or the presence of filling defects. An additional group of patients who have cholesterosis, cholecystitis, or cholelithiasis missed by the oral cholecystogram will not be diagnosed by cholecystokinin cholangiography unless the duodenal bile is also examined.", "contents": "Cholecystokinin cholangiography and analysis of duodenal bile in the investigation of pain in the right upper quadrant of the abdomen without gallstones. Thirty-one patients with recurrent symptoms of the biliary tract and repeated normal oral cholecystograms were studied by a combination of cholecystokinin cholangiography and biliary drainage. Ten patients had reduplication of their symptons because of dyskinetic contractions or obstruction of the cystic duct, and seven patients had delayed gallbladder emptying without pain due to hypokinetic contractions. Five patients had abnormal duodenal bile characterized by supersaturation and the presence of crystals or bacteria. Based upon these studies, 22 patients had cholecystectomy and 20 were cured, while two showed improvement. There were no therapeutic failures. Cholecystokinin cholangiography capably detects the presence of neuromuscular disease of the gallbladder wall, whereas the oral cholecystogram tests for mucosal function or the presence of filling defects. An additional group of patients who have cholesterosis, cholecystitis, or cholelithiasis missed by the oral cholecystogram will not be diagnosed by cholecystokinin cholangiography unless the duodenal bile is also examined."}
{"id": "PMID:1090020", "title": "Subpopulations of thymus dependent and thymus independent lymphocytes in human gut associated lymphoid tissues.", "content": "Human gut associated lymphoid tissues were studied in terms of the presence of subpopulations of thymus dependent and thymus independent lymphocytes. Both thymus dependent and thymus independent lymphocytes were present in substantial numbers, as judged from their ability to form spontaneous and complement mediated rosettes with sheep red blood cells and the presence of surface bound immunoglobulins. The relationship between this finding and the question of what is the lymphoid organ which may be the human equivalent of the avian bursa is discussed.", "contents": "Subpopulations of thymus dependent and thymus independent lymphocytes in human gut associated lymphoid tissues. Human gut associated lymphoid tissues were studied in terms of the presence of subpopulations of thymus dependent and thymus independent lymphocytes. Both thymus dependent and thymus independent lymphocytes were present in substantial numbers, as judged from their ability to form spontaneous and complement mediated rosettes with sheep red blood cells and the presence of surface bound immunoglobulins. The relationship between this finding and the question of what is the lymphoid organ which may be the human equivalent of the avian bursa is discussed."}
{"id": "PMID:1090022", "title": "Has medical history importance for surgeons?", "content": "Surgeons will do well to remember that the two most important contributions to the growth and extension of surgery came from two disciplines, not then regarded as the most innovative. Anesthesia came from dentistry, the work primarily of W.T.G. Morton of Boston; prophylactic surgical antisepsis originated with the obstetrician Semmelweis, who developed a scheme of prophylactic chemical antisepsis that still remains the core of surgical antisepsis. In the mid 1880's, largely as a result of the work of Chamberland and others of the Pasteur school, surgeons in France and Germany substituted thermal for chemical antisepsis, whenever applicable. Whereas Lister's influence was tremendous in fostering acceptance of antisepsis by surgeons, by the end of his professorial career he had begun his capitulation to prophylactic antisepsis, which was complete by 1896 to the very practices that Semmelweis had proved the value of almost five decades previously. These were 19th century innovations. The greatest boon to surgery's advance in this century has been control of cellulitic infections through chemotherapeutic agencies, the sulfonamides and antibiotics. The tremendous upsurge of interest in research at the end of World War II brought surgeons to a fuller realization of the significant part they could play in the advance of their discipline. Intimate alignment of surgeons with physiologists of the circulation begot intracardiac surgery, a significant innovation with consequences of tremendous import for greater medicine's advance. Today, surgeons attacking the problem of tissue transplantation are aligning themselves with biochemists, geneticists, immunologists, experimental pathologists, and pharmacologists in their broad approach to the phenomenon of allograft rejection. The great extension of vascular surgery since World War II has made jewelers of surgeons of small tubular structures. The technical phases of these demanding operative procedures have largely been overcome. Solution of the biologic rejection phenomenon is awaited eagerly by all investigators, a discovery that will greatly enhance predictable success of transfer of skin as well as of organs. When will surgery experience another great catalytic forward thrust like that achieved through anesthesia, prophylactic antisepsis, and the antibiotics? No discipline in medicine can exist alone without privation. For its continuing advancement, surgery is dependent upon close and intimate relationships with many other medical disciplines. Apart from the enlightenment provided by a searching examination of the origins of our surgical discipline, the earnest and persistent pursuer will discover a lively pleasure and satisfaction that accrues as a special dividend.", "contents": "Has medical history importance for surgeons? Surgeons will do well to remember that the two most important contributions to the growth and extension of surgery came from two disciplines, not then regarded as the most innovative. Anesthesia came from dentistry, the work primarily of W.T.G. Morton of Boston; prophylactic surgical antisepsis originated with the obstetrician Semmelweis, who developed a scheme of prophylactic chemical antisepsis that still remains the core of surgical antisepsis. In the mid 1880's, largely as a result of the work of Chamberland and others of the Pasteur school, surgeons in France and Germany substituted thermal for chemical antisepsis, whenever applicable. Whereas Lister's influence was tremendous in fostering acceptance of antisepsis by surgeons, by the end of his professorial career he had begun his capitulation to prophylactic antisepsis, which was complete by 1896 to the very practices that Semmelweis had proved the value of almost five decades previously. These were 19th century innovations. The greatest boon to surgery's advance in this century has been control of cellulitic infections through chemotherapeutic agencies, the sulfonamides and antibiotics. The tremendous upsurge of interest in research at the end of World War II brought surgeons to a fuller realization of the significant part they could play in the advance of their discipline. Intimate alignment of surgeons with physiologists of the circulation begot intracardiac surgery, a significant innovation with consequences of tremendous import for greater medicine's advance. Today, surgeons attacking the problem of tissue transplantation are aligning themselves with biochemists, geneticists, immunologists, experimental pathologists, and pharmacologists in their broad approach to the phenomenon of allograft rejection. The great extension of vascular surgery since World War II has made jewelers of surgeons of small tubular structures. The technical phases of these demanding operative procedures have largely been overcome. Solution of the biologic rejection phenomenon is awaited eagerly by all investigators, a discovery that will greatly enhance predictable success of transfer of skin as well as of organs. When will surgery experience another great catalytic forward thrust like that achieved through anesthesia, prophylactic antisepsis, and the antibiotics? No discipline in medicine can exist alone without privation. For its continuing advancement, surgery is dependent upon close and intimate relationships with many other medical disciplines. Apart from the enlightenment provided by a searching examination of the origins of our surgical discipline, the earnest and persistent pursuer will discover a lively pleasure and satisfaction that accrues as a special dividend."}
{"id": "PMID:1090034", "title": "Cryostat microtomy of lung tissue in an expanded state.", "content": "A technique is reported for cryostat sectioning of lung tissue in an expanded state for use in viral immunofluorescence studies. A 1:2 mixture of O.C.T. embedding compound and phosphate-buffered saline is injected intratracheally into fresh lung tissue. The lung tissue is frozen in liquid nitrogen and sectioned with a cryostat. Compared to other published reports of lung sectioning for immunofluorescence microscopy, this method has the advantages of being easy and quick, maintaining the lung sections in an expanded rather than collapsed state and avoiding contact with chemicals potentially capable of altering sensitive viral antigen.", "contents": "Cryostat microtomy of lung tissue in an expanded state. A technique is reported for cryostat sectioning of lung tissue in an expanded state for use in viral immunofluorescence studies. A 1:2 mixture of O.C.T. embedding compound and phosphate-buffered saline is injected intratracheally into fresh lung tissue. The lung tissue is frozen in liquid nitrogen and sectioned with a cryostat. Compared to other published reports of lung sectioning for immunofluorescence microscopy, this method has the advantages of being easy and quick, maintaining the lung sections in an expanded rather than collapsed state and avoiding contact with chemicals potentially capable of altering sensitive viral antigen."}
{"id": "PMID:1090041", "title": "[Differentiation of urinary bacteria in urological practice].", "content": "The value of a new differential diagnostic nutrient medium was tested on 360 urines from urological patients. Simultaneously every urine specimen was examined according to classical bacteriological differential methods and colony counting. In 137 sterile urines there was agreement between the two methods in approximately 93%. On the basis of colony counting 96% of the 223 urinary tract infections could be diagnosed with the new test. Differentiation of the infecting organisms was possible in 71% of the infections.", "contents": "[Differentiation of urinary bacteria in urological practice]. The value of a new differential diagnostic nutrient medium was tested on 360 urines from urological patients. Simultaneously every urine specimen was examined according to classical bacteriological differential methods and colony counting. In 137 sterile urines there was agreement between the two methods in approximately 93%. On the basis of colony counting 96% of the 223 urinary tract infections could be diagnosed with the new test. Differentiation of the infecting organisms was possible in 71% of the infections."}
{"id": "PMID:1090042", "title": "Renal artery aneurysm. Polyaneurysmal lesion of kidney.", "content": "A case of innumerable renal artery aneurysms of probable congenital origin is reported. No other lesion could be found in any other abdominal organ studied. We think it unique as no similar case could be found in the medical literature. Except for right flank malaise, the patient was otherwise asymptomatic. The radiologic diagnostic methods are discussed, stressing the differential diagnosis of calcifications in the area of the kidney. The management of renal aneurysms is discussed in regard to the nephrectomy.", "contents": "Renal artery aneurysm. Polyaneurysmal lesion of kidney. A case of innumerable renal artery aneurysms of probable congenital origin is reported. No other lesion could be found in any other abdominal organ studied. We think it unique as no similar case could be found in the medical literature. Except for right flank malaise, the patient was otherwise asymptomatic. The radiologic diagnostic methods are discussed, stressing the differential diagnosis of calcifications in the area of the kidney. The management of renal aneurysms is discussed in regard to the nephrectomy."}
{"id": "PMID:1090043", "title": "Scrotal pouch techniques. Adjunct to orchiopexy.", "content": "Following orchiopexy, contraction of dartos and cremaster muscles may pull the testis toward the external inguinal ring. Fibrous tissue deposition resulting from testis mobilization and cord skeletonization may bind the testis in an unfavorably high scrotal position. The \"scrotal pouch technique,\" utilizing both a subcutaneous scrotal pocket for the testis external to the dartos layer and a gentle testis traction regimen, serves to diminish the chances of testis retraction after orchiopexy.", "contents": "Scrotal pouch techniques. Adjunct to orchiopexy. Following orchiopexy, contraction of dartos and cremaster muscles may pull the testis toward the external inguinal ring. Fibrous tissue deposition resulting from testis mobilization and cord skeletonization may bind the testis in an unfavorably high scrotal position. The \"scrotal pouch technique,\" utilizing both a subcutaneous scrotal pocket for the testis external to the dartos layer and a gentle testis traction regimen, serves to diminish the chances of testis retraction after orchiopexy."}
{"id": "PMID:1090044", "title": "Renal transplantation. Complications in patients with juvenile diabetes.", "content": "Four cases of patients with juvenile diabetes who received kidney transplants are reported. Problems with selection and management are discussed, and the high mortality and morbidity rates in this group of patients is emphasized.", "contents": "Renal transplantation. Complications in patients with juvenile diabetes. Four cases of patients with juvenile diabetes who received kidney transplants are reported. Problems with selection and management are discussed, and the high mortality and morbidity rates in this group of patients is emphasized."}
{"id": "PMID:1090045", "title": "Renal transplantation. In adult patients with end stage polycystic kidney disease.", "content": "Seventeen adult patients with end stage polycystic kidney disease underwent renal transplantation. Two groups were identified: (1) those transplanted with retained polycystic kidneys, (2) those prepared with preliminary bilateral nephrectomy. Although there was a shockingly higher incidence in the mortality rate in the non-nephrectomized group, no specific cause could be identified. The results do indicate, however, that bilateral nephrectomy is essential in all patients with a history of pyelonephritis or gross hematuria. Substantial benefit from retained kidneys was noted only during the initial period of hemodialysis.", "contents": "Renal transplantation. In adult patients with end stage polycystic kidney disease. Seventeen adult patients with end stage polycystic kidney disease underwent renal transplantation. Two groups were identified: (1) those transplanted with retained polycystic kidneys, (2) those prepared with preliminary bilateral nephrectomy. Although there was a shockingly higher incidence in the mortality rate in the non-nephrectomized group, no specific cause could be identified. The results do indicate, however, that bilateral nephrectomy is essential in all patients with a history of pyelonephritis or gross hematuria. Substantial benefit from retained kidneys was noted only during the initial period of hemodialysis."}
{"id": "PMID:1090046", "title": "Calculi complicating renal transplantation into ileal conduits.", "content": "In 2 patients with renal transplants into ileal conduits renal calculi developed proximal to stenosis of the ureteral-ileal anastomosis, These are the only cases of urinary calculi in 600 consecutive transplants. The pathogenetic factors contributing to sepsis and stasis in such cases are reviewed and precautions suggested.", "contents": "Calculi complicating renal transplantation into ileal conduits. In 2 patients with renal transplants into ileal conduits renal calculi developed proximal to stenosis of the ureteral-ileal anastomosis, These are the only cases of urinary calculi in 600 consecutive transplants. The pathogenetic factors contributing to sepsis and stasis in such cases are reviewed and precautions suggested."}
{"id": "PMID:1090047", "title": "Quantitative urine culture by reagent strip incubation.", "content": "The filter paper culture-tetrazolium test ressults have been compared with routine hospital streak plate cultures in 400 random urine specimens. Pseudomonas cultures tended to impart a color change to the test pads which rendered interpretation difficult. While a consistently accurate quantitative interpretation of these cultures did emerge during the course of the trial studies, some degree of observer accommodation and bias, in terms of the manufacturer's instructions and description, was necessary to achieve an appropriate reading. When the hospital laboratory results and culture strip test results are compared in terms of colony counts greater than 10-4 bacteria per milliliter and less than 10-4 bacteria per milliliter, the method was found to be 93% sensitive for the total count and 83% sensitive for the gram-negative count with a specificity correlation of 96% for the total and gram-negative counts, respectively.", "contents": "Quantitative urine culture by reagent strip incubation. The filter paper culture-tetrazolium test ressults have been compared with routine hospital streak plate cultures in 400 random urine specimens. Pseudomonas cultures tended to impart a color change to the test pads which rendered interpretation difficult. While a consistently accurate quantitative interpretation of these cultures did emerge during the course of the trial studies, some degree of observer accommodation and bias, in terms of the manufacturer's instructions and description, was necessary to achieve an appropriate reading. When the hospital laboratory results and culture strip test results are compared in terms of colony counts greater than 10-4 bacteria per milliliter and less than 10-4 bacteria per milliliter, the method was found to be 93% sensitive for the total count and 83% sensitive for the gram-negative count with a specificity correlation of 96% for the total and gram-negative counts, respectively."}
{"id": "PMID:1090048", "title": "Sexual function of males before and after renal transplantation.", "content": "In an attempt to define changes in sexual function before and after renal transplantation, a questionnaire was distributed to adult male patients with functioning kidney transplants. Significant changes in sexual performance were shown during the transition from the healthy state to end-stage renal failure and, finally, to the post-transplant state. Both potency and libido showed marked parallel impairment with the development of end-stage renal failure, followed by moderate recovery after renal transplantation. This study indicates that the majority of men with functioning kidneys can look forward to a return of sexual activity comparable to pre-illness level. However, sexual impairment may persist in some patients after transplantation, emphasizing the need for further evaluation in this group of patients.", "contents": "Sexual function of males before and after renal transplantation. In an attempt to define changes in sexual function before and after renal transplantation, a questionnaire was distributed to adult male patients with functioning kidney transplants. Significant changes in sexual performance were shown during the transition from the healthy state to end-stage renal failure and, finally, to the post-transplant state. Both potency and libido showed marked parallel impairment with the development of end-stage renal failure, followed by moderate recovery after renal transplantation. This study indicates that the majority of men with functioning kidneys can look forward to a return of sexual activity comparable to pre-illness level. However, sexual impairment may persist in some patients after transplantation, emphasizing the need for further evaluation in this group of patients."}
{"id": "PMID:1090049", "title": "Urinary incontinence. Treatment with electrical stimulation of the pelvic floor.", "content": "Electrical pelvic floor stimulation may be administered either externally by the transrectal tampon or internally with the implantable pelvic floor stimulator. This treatment modality requires intact pelvic floor innervation and therefore is unsuccessful in patients with pelvic floor denervation. Pelvic floor stimulation has been successful in patients with stress and with congenital, iatrogenic, and postoperative urinary incontinence.", "contents": "Urinary incontinence. Treatment with electrical stimulation of the pelvic floor. Electrical pelvic floor stimulation may be administered either externally by the transrectal tampon or internally with the implantable pelvic floor stimulator. This treatment modality requires intact pelvic floor innervation and therefore is unsuccessful in patients with pelvic floor denervation. Pelvic floor stimulation has been successful in patients with stress and with congenital, iatrogenic, and postoperative urinary incontinence."}
{"id": "PMID:1090050", "title": "Ureteral replacements.", "content": "The clinical necessities of replacing all or part of the diseased or destroyed ureter have plagued surgeons for many years. In the past seventy years more than 100 surgical techniques have been described for ureteral replacement. Methods of replacement have fallen into four categories; nonbiologic substitutes, pedicle grafts, free grafts, and implantation and diversion. This review discusses the history and present status, and presents a look to the future in the surgery of ureteral replacement.", "contents": "Ureteral replacements. The clinical necessities of replacing all or part of the diseased or destroyed ureter have plagued surgeons for many years. In the past seventy years more than 100 surgical techniques have been described for ureteral replacement. Methods of replacement have fallen into four categories; nonbiologic substitutes, pedicle grafts, free grafts, and implantation and diversion. This review discusses the history and present status, and presents a look to the future in the surgery of ureteral replacement."}
{"id": "PMID:1090051", "title": "Urinary tract calculi associated with enteritis and intestinal bypass.", "content": "There is a definite increased incidence of calculi associated with extensive small bowel disease or resection. Hyperoxaluria appears to play a major role and may be due to increased intestinal oxalate absorption. Forced fluids and dietary discretion are mainstays of stone prophylaxis.", "contents": "Urinary tract calculi associated with enteritis and intestinal bypass. There is a definite increased incidence of calculi associated with extensive small bowel disease or resection. Hyperoxaluria appears to play a major role and may be due to increased intestinal oxalate absorption. Forced fluids and dietary discretion are mainstays of stone prophylaxis."}
{"id": "PMID:1090052", "title": "Live donor nephrectomy.", "content": "Donor nephrectomy was performed on 39 living related renal donors. The preoperative evaluation, surgical technique, and postoperative follow-up are presented. Major or minor complications developed in 23 per cent of the patients and included acute myocardial infarction, brachial plexus injury, acute glomerulonephritis, ileus, pneumonia, and urinary tract infection. All complications had resolved by six months postoperatively.", "contents": "Live donor nephrectomy. Donor nephrectomy was performed on 39 living related renal donors. The preoperative evaluation, surgical technique, and postoperative follow-up are presented. Major or minor complications developed in 23 per cent of the patients and included acute myocardial infarction, brachial plexus injury, acute glomerulonephritis, ileus, pneumonia, and urinary tract infection. All complications had resolved by six months postoperatively."}
{"id": "PMID:1090053", "title": "Urologic complications in renal transplantation.", "content": "Our ten-year experience with 141 kidney transplants is reported. Fifty per cent of the grafts are presently functioning, and 22 patients have been followed more than five years. A urologic complication rate of 13 per cent occurred, but 84 per cent of those patients retained their grafts. Only 1.3 per cent of patients with urologic complications died. Improved results can be achieved by good operative technique and aggressive management of complications.", "contents": "Urologic complications in renal transplantation. Our ten-year experience with 141 kidney transplants is reported. Fifty per cent of the grafts are presently functioning, and 22 patients have been followed more than five years. A urologic complication rate of 13 per cent occurred, but 84 per cent of those patients retained their grafts. Only 1.3 per cent of patients with urologic complications died. Improved results can be achieved by good operative technique and aggressive management of complications."}
{"id": "PMID:1090055", "title": "Testicular lymphoma.", "content": "Testicular tumor in the sixty- to eighty-year age group is mostly lymphoma. It is usually a manifestation of a generalized lymphoma, even though it may appear localized. Prognosis is uniformly poor. A combined approach of radiation and chemotherapy gives the best results. Chemotherapy is used sequentially in multiple doses. Two cases are reported.", "contents": "Testicular lymphoma. Testicular tumor in the sixty- to eighty-year age group is mostly lymphoma. It is usually a manifestation of a generalized lymphoma, even though it may appear localized. Prognosis is uniformly poor. A combined approach of radiation and chemotherapy gives the best results. Chemotherapy is used sequentially in multiple doses. Two cases are reported."}
{"id": "PMID:1090056", "title": "Nephrogenic adenoma of bladder in immunosuppressed renal transplantation.", "content": "A case of nephrogenic adenoma is reported in an immunosuppressed renal transplant recipient. The nature of the lesion is discussed, and a possible relationship between benign tumors and impaired immunologic surveillance is considered.", "contents": "Nephrogenic adenoma of bladder in immunosuppressed renal transplantation. A case of nephrogenic adenoma is reported in an immunosuppressed renal transplant recipient. The nature of the lesion is discussed, and a possible relationship between benign tumors and impaired immunologic surveillance is considered."}
{"id": "PMID:1090065", "title": "The induction, persistence and loss of framycetin sulphate resistance in enteric E coli of pigs.", "content": "When framycetin sulphate was fed at prophylactic levels to pigs, resistance to this antibiotic was induced within days in the enteric E coli. The E coli of the gut did not become fully susceptible again until six to seven weeks after the anitbiotic was withdrawn from the diet.", "contents": "The induction, persistence and loss of framycetin sulphate resistance in enteric E coli of pigs. When framycetin sulphate was fed at prophylactic levels to pigs, resistance to this antibiotic was induced within days in the enteric E coli. The E coli of the gut did not become fully susceptible again until six to seven weeks after the anitbiotic was withdrawn from the diet."}
{"id": "PMID:1090069", "title": "Effects of domestication on animal behaviour.", "content": "Centuries of domestication of animals by civilised man have had many measurable effects on the various species involved, but only in relatively recent history has scientific curiosity been directed to assessing their extent. The process of domestication is analysed and its known effects on animals reviewed through observations of and experiments with various species of domesticated animals conducted by researchers into animal psychology and biology. The known facts on the effects of domestication in animals are extrapolated in an attempt to determine to what extent modern man himself has been domesticated in the urban environment.", "contents": "Effects of domestication on animal behaviour. Centuries of domestication of animals by civilised man have had many measurable effects on the various species involved, but only in relatively recent history has scientific curiosity been directed to assessing their extent. The process of domestication is analysed and its known effects on animals reviewed through observations of and experiments with various species of domesticated animals conducted by researchers into animal psychology and biology. The known facts on the effects of domestication in animals are extrapolated in an attempt to determine to what extent modern man himself has been domesticated in the urban environment."}
{"id": "PMID:1090089", "title": "Serological classification of anti-I sera.", "content": "Twelve anti-I sera were examined by means of broad panel of cells: OI, BI, OhI, Oicord, Oiadult, BI-F. Titration, fixation-elution, and haemagglutination-inhibition tests, and comparison of agglutination of untreated and neuraminidase-treated erythrocytes allowed to establish the specificity of the sera. For some sera the serological heterogeneity was revealed, which consisted in the following combinations of two or more kinds of specificity: anti-I-D plus anti-I-F, anti-I-D plus anti-i, anti-I-D plus anti-I-S, anti-I-D plus anti-I-F plus anti-I-S. Methodical problems of classification of anti-I sera are discussed.", "contents": "Serological classification of anti-I sera. Twelve anti-I sera were examined by means of broad panel of cells: OI, BI, OhI, Oicord, Oiadult, BI-F. Titration, fixation-elution, and haemagglutination-inhibition tests, and comparison of agglutination of untreated and neuraminidase-treated erythrocytes allowed to establish the specificity of the sera. For some sera the serological heterogeneity was revealed, which consisted in the following combinations of two or more kinds of specificity: anti-I-D plus anti-I-F, anti-I-D plus anti-i, anti-I-D plus anti-I-S, anti-I-D plus anti-I-F plus anti-I-S. Methodical problems of classification of anti-I sera are discussed."}
{"id": "PMID:1090097", "title": "Hans Schaefer's asystole: two case reports.", "content": "Two cases of myocardial infarction are reported with transient (case 1) or permanent (Case 2) recovery of an R wave in anterior chest leads in the acute epsiode. This observation confirms H. Schaefer's hypothesis of asystole of the affected area, i.e. electrical inexcitability without necrosis. This phase precedes necrosis in any acute infarction; in the large majority of cases, myocardial infarction ultimately develops; therefore, demonstration in a clinical follow-up is quite rare.", "contents": "Hans Schaefer's asystole: two case reports. Two cases of myocardial infarction are reported with transient (case 1) or permanent (Case 2) recovery of an R wave in anterior chest leads in the acute epsiode. This observation confirms H. Schaefer's hypothesis of asystole of the affected area, i.e. electrical inexcitability without necrosis. This phase precedes necrosis in any acute infarction; in the large majority of cases, myocardial infarction ultimately develops; therefore, demonstration in a clinical follow-up is quite rare."}
{"id": "PMID:1090098", "title": "[Availability of cryo-solutions for use in a thermodilution method].", "content": "A simple device was developed allowing the storage of cooled injectate at constant temperature. Cardiac output determinations by the thermodilution technique are simplified considerably.", "contents": "[Availability of cryo-solutions for use in a thermodilution method]. A simple device was developed allowing the storage of cooled injectate at constant temperature. Cardiac output determinations by the thermodilution technique are simplified considerably."}
{"id": "PMID:1090099", "title": "LH and FSH in serum after intramuscular administration of LH/FSH-releasing hormone in normal and hypogonadal men.", "content": "The increase in serum concentrations of LH and FSH after intramuscular administration of LH/FSH-RH was investigated in normal and hypogonadotrophic hypogonadal males. LH and FSH were measured by specific radio-immunoassays. To evaluate the appropriate dose of LH/FSH-RH for intramuscular administration different doses were administered and with 200 mu g an increase in LH and FSH was found comparable to the results obtained by other investigators using intravenous administration. After the initial studies a simple test including two determinations before LH/FSH-RH administration and one 60 min and 120 min after administration was developed and used in all cases. In 12 normal males a significant increase in the serum concentration of both LH and FSH was found averaging 381% and 148% respectively 60 min after 200 mu g LH/FSH-RH with a slight decrease in LH but not in FSH after 120 min. A positive response similar to that in normal men was observed in 11 hypogonadotrophic hypogonadal patients presenting different clinical pictures. This indicates that in all these patients the hypogonadotrophic state was due to a hypothalamic defect and not to a hypophyseal disorder.", "contents": "LH and FSH in serum after intramuscular administration of LH/FSH-releasing hormone in normal and hypogonadal men. The increase in serum concentrations of LH and FSH after intramuscular administration of LH/FSH-RH was investigated in normal and hypogonadotrophic hypogonadal males. LH and FSH were measured by specific radio-immunoassays. To evaluate the appropriate dose of LH/FSH-RH for intramuscular administration different doses were administered and with 200 mu g an increase in LH and FSH was found comparable to the results obtained by other investigators using intravenous administration. After the initial studies a simple test including two determinations before LH/FSH-RH administration and one 60 min and 120 min after administration was developed and used in all cases. In 12 normal males a significant increase in the serum concentration of both LH and FSH was found averaging 381% and 148% respectively 60 min after 200 mu g LH/FSH-RH with a slight decrease in LH but not in FSH after 120 min. A positive response similar to that in normal men was observed in 11 hypogonadotrophic hypogonadal patients presenting different clinical pictures. This indicates that in all these patients the hypogonadotrophic state was due to a hypothalamic defect and not to a hypophyseal disorder."}
{"id": "PMID:1090100", "title": "Hypothalamic-pituitary function in anorexia nervosa.", "content": "Hypothalamic-pituitary function was studied in 4 patients with anorexia nervosa of different degree of severity before and after refeeding. Gonadotrophin secretion was low in all subjects and improved in two after a prolonged period of feeding. In one subject there was a failure of pituitary gonadotrophin secretion after the administration of hypothalamic gonadotrophin-releasing hormone but the response was restored to normal after treatment. Thyroid function was reduced in one patient only but returned to normal after intravenous therapy for 6 days. Growth hormone and cortisol levels were elevated in all patients, and in one severe case the growth hormone values were extremely high. There was also a disturbance of the hypothalamic control of growth hormone and pituitary-adrenal function, which returned to normal after refeeding. One patient with severe hypothermia was resistant to the administration of a pyrogen, but developed a normal febrile response after treatment.", "contents": "Hypothalamic-pituitary function in anorexia nervosa. Hypothalamic-pituitary function was studied in 4 patients with anorexia nervosa of different degree of severity before and after refeeding. Gonadotrophin secretion was low in all subjects and improved in two after a prolonged period of feeding. In one subject there was a failure of pituitary gonadotrophin secretion after the administration of hypothalamic gonadotrophin-releasing hormone but the response was restored to normal after treatment. Thyroid function was reduced in one patient only but returned to normal after intravenous therapy for 6 days. Growth hormone and cortisol levels were elevated in all patients, and in one severe case the growth hormone values were extremely high. There was also a disturbance of the hypothalamic control of growth hormone and pituitary-adrenal function, which returned to normal after refeeding. One patient with severe hypothermia was resistant to the administration of a pyrogen, but developed a normal febrile response after treatment."}
{"id": "PMID:1090101", "title": "Further studies on the nature of the immunoreactive luteinizing hormone-releasing hormone (LH-RH)-like peptide in human urine.", "content": "The chemical nature of the immunoreactive LH-RH-like peptide found in human urine has been investigated using ion-exchange chromatography on carboxymethyl (CM)-cellulose and a radioimmunoassay for LH-RH. A single immunoreactive substance was found in urine after LH-RH administration and in urine samples from untreated subjects. This substance did not have the mobility of either the synthetic decapeptide nor the 3-10 octapeptide on CM-cellulose and the evidence suggests that it may be the 2-10 nonapeptide of LH-RH.", "contents": "Further studies on the nature of the immunoreactive luteinizing hormone-releasing hormone (LH-RH)-like peptide in human urine. The chemical nature of the immunoreactive LH-RH-like peptide found in human urine has been investigated using ion-exchange chromatography on carboxymethyl (CM)-cellulose and a radioimmunoassay for LH-RH. A single immunoreactive substance was found in urine after LH-RH administration and in urine samples from untreated subjects. This substance did not have the mobility of either the synthetic decapeptide nor the 3-10 octapeptide on CM-cellulose and the evidence suggests that it may be the 2-10 nonapeptide of LH-RH."}
{"id": "PMID:1090102", "title": "The postnatal occurrence of biogenic monoamines in pancreatic islets of golden hamsters.", "content": "Fluorescence histochemical studies in newborn and young golden hamster have revealed that some cells in the islets of Langerhans contain monoamine. These cells can be seen in newborns and up to ten day old animals, at least in three to five days old animals in the amine behaves like dopamine and is mostly stored in the A2-cells.", "contents": "The postnatal occurrence of biogenic monoamines in pancreatic islets of golden hamsters. Fluorescence histochemical studies in newborn and young golden hamster have revealed that some cells in the islets of Langerhans contain monoamine. These cells can be seen in newborns and up to ten day old animals, at least in three to five days old animals in the amine behaves like dopamine and is mostly stored in the A2-cells."}
{"id": "PMID:1090103", "title": "A double-blind trial with baclofen (Lioresal) and diazepam in spasticity due to multiple sclerosis.", "content": "In 17 in-patients suffering from spasticity due to multiple sclerosis, the effect and tolerability periods were 4 weeks each. As to efficacy, the variables: spasticity, clonus, flexor spasms, gait and bladder function were evaluated clinically. No significant difference was found between the two drugs. As far as side-effects are concerned, sedation was specifically inquired about. Apart from that, spontaneoulsy reported side-effects were recorded. Sedation was more often seen during treatment with diazepam, while the side-effects during baclofen treatment were more varied. The total number and severity of side-effects were equal in the two treatment groups. A preference for one of the two treatment periods was stated by the investigator before the code was broken. A significant difference (p less than 0.001) in favor of Lioresal was found. This is discussed in the light of the fact that no significant difference was found for the individual symptoms or side-effects.", "contents": "A double-blind trial with baclofen (Lioresal) and diazepam in spasticity due to multiple sclerosis. In 17 in-patients suffering from spasticity due to multiple sclerosis, the effect and tolerability periods were 4 weeks each. As to efficacy, the variables: spasticity, clonus, flexor spasms, gait and bladder function were evaluated clinically. No significant difference was found between the two drugs. As far as side-effects are concerned, sedation was specifically inquired about. Apart from that, spontaneoulsy reported side-effects were recorded. Sedation was more often seen during treatment with diazepam, while the side-effects during baclofen treatment were more varied. The total number and severity of side-effects were equal in the two treatment groups. A preference for one of the two treatment periods was stated by the investigator before the code was broken. A significant difference (p less than 0.001) in favor of Lioresal was found. This is discussed in the light of the fact that no significant difference was found for the individual symptoms or side-effects."}
{"id": "PMID:1090106", "title": "Successfully treated pneumopericardium in a newborn infant during IPPV.", "content": "The successful treatment of pneumopericardium and cardiac tamponade in a newborn infant is presented in this case report. Alert recognition of this complication in newborn infants on intermittent positive pressure ventilation (IPPV) is of the utmost importance as prompt and adequate treatment is life-saving.", "contents": "Successfully treated pneumopericardium in a newborn infant during IPPV. The successful treatment of pneumopericardium and cardiac tamponade in a newborn infant is presented in this case report. Alert recognition of this complication in newborn infants on intermittent positive pressure ventilation (IPPV) is of the utmost importance as prompt and adequate treatment is life-saving."}
{"id": "PMID:1090107", "title": "Treatment of meningitis and septicemia in infancy with a sulphamethoxazole/trimethorpim combination.", "content": "Ten infants, 8 days to 10 months old, with meningitis and/or septicemia were considered therapeutic failures after conventional antibiotic treatment (i.e. kanamycin, ampicillin and sulfonamides) and given sulphamethoxazole and trimethoprim parenterally. Nine patients recovered, 8 of them rapidly, and one after prolonged treatment for 34 days when kanamycin was added to the combination. One infant improved but later died of complications not related to the treatment. High concentrations in serum and cerebrospinal fluid were achieved with a daily dose of 30-40 mg sulphamethoxazole and 6-8 mg trimethoprim per kg without signs of accumulation. No change in resistance of the bacteria isolated was seen. A hemolytic reaction, probably due to the propylene glycol in the solution, was seen in one case. Other possible side-effects in this age-group are discussed. The antibiotic combination used seems to be a good alternative in the therapy of bacterial meningitis of infants caused by gram-negative bacteria. However it should still not be given to icteric or very immature infants and probably not during the first week of life.", "contents": "Treatment of meningitis and septicemia in infancy with a sulphamethoxazole/trimethorpim combination. Ten infants, 8 days to 10 months old, with meningitis and/or septicemia were considered therapeutic failures after conventional antibiotic treatment (i.e. kanamycin, ampicillin and sulfonamides) and given sulphamethoxazole and trimethoprim parenterally. Nine patients recovered, 8 of them rapidly, and one after prolonged treatment for 34 days when kanamycin was added to the combination. One infant improved but later died of complications not related to the treatment. High concentrations in serum and cerebrospinal fluid were achieved with a daily dose of 30-40 mg sulphamethoxazole and 6-8 mg trimethoprim per kg without signs of accumulation. No change in resistance of the bacteria isolated was seen. A hemolytic reaction, probably due to the propylene glycol in the solution, was seen in one case. Other possible side-effects in this age-group are discussed. The antibiotic combination used seems to be a good alternative in the therapy of bacterial meningitis of infants caused by gram-negative bacteria. However it should still not be given to icteric or very immature infants and probably not during the first week of life."}
{"id": "PMID:1090108", "title": "Pulmonary mechanics in infants surviving severe neonatal respiratory insufficiency.", "content": "Pulmonary mechanics was studied in 24 survivors of severe neonatal ventilatory insufficiency, 15 infants had idiopathic respiratory distress syndrome (IRDS), 6 recurrent severe apnoeic spells, and 3 postasphyxia syndrome. Of the infants with IRDS, 5 were treated with intermittent positive pressure ventilation (IPPV), 3 with continuous positive airway pressure (CPAP) via an endotracheal tube and 7 with CPAP applied via a face chamber. The other infants were all treated with IPPV. IPPV-treated infants generally had lower than expected values of dynamic compliance and pulmonary conductance, particularly after prolonged treatment. All infants treated with CPAP via a face chamber had normal mechanics, but a trend towards obstruction of the airways after varying periods of time was observed in most infants, irrespective of diagnosis or treatment. One infant treated with CPAP via an endotracheal tube and given pure oxygen for a long time had gross abnormalities suggesting bronchopulmonary dysplasia. Measurement of pulmonary conductance appears to be a reliable prognostic tool as concerns pulmonary symptoms later in infancy.", "contents": "Pulmonary mechanics in infants surviving severe neonatal respiratory insufficiency. Pulmonary mechanics was studied in 24 survivors of severe neonatal ventilatory insufficiency, 15 infants had idiopathic respiratory distress syndrome (IRDS), 6 recurrent severe apnoeic spells, and 3 postasphyxia syndrome. Of the infants with IRDS, 5 were treated with intermittent positive pressure ventilation (IPPV), 3 with continuous positive airway pressure (CPAP) via an endotracheal tube and 7 with CPAP applied via a face chamber. The other infants were all treated with IPPV. IPPV-treated infants generally had lower than expected values of dynamic compliance and pulmonary conductance, particularly after prolonged treatment. All infants treated with CPAP via a face chamber had normal mechanics, but a trend towards obstruction of the airways after varying periods of time was observed in most infants, irrespective of diagnosis or treatment. One infant treated with CPAP via an endotracheal tube and given pure oxygen for a long time had gross abnormalities suggesting bronchopulmonary dysplasia. Measurement of pulmonary conductance appears to be a reliable prognostic tool as concerns pulmonary symptoms later in infancy."}
{"id": "PMID:1090109", "title": "The immune response to urinary tract infections in childhood. I. Serological diagnosis of primary symptomatic infection in girls by indirect hemagglutination.", "content": "Determination of antibodies to E. Coli O antigen by indirect hemagglutination for detection of acute pyelonephritis was investigated in sequences of sera from 94 girls with their first known attack of symptomatic urinary tract infection. Using O antigen from the infecting bacterial strain or a standard strain increased antibody titres were found among pyelonephritis patients in 89% in unreduced sera (mainly 19 S antibodies) and in 81% in reduced sera (mainly 7 S antibodies) compared to a control material of 643 healthy children. Significant changes of these antibody titres occurred in 74 and 39% respectively. Analysis of maximal titres as well as titre changes in both unreduced and reduced sera showed diagnostically significant changes in two or more of these four parameters in 89% of patients with pyelonephritis. In contrast one parameter with such changes was found among the cystitis patients in only 5%. Employing a pool of 8 common O antigens, increased antibody titres were detected in 76% of patients with pyelonephritis. A larger pool of 68 antigens was no better and had a low capacity to detect 7 S antibodies. Optimal time for blood sampling was 2 to 3 weeks after onset of symptoms.", "contents": "The immune response to urinary tract infections in childhood. I. Serological diagnosis of primary symptomatic infection in girls by indirect hemagglutination. Determination of antibodies to E. Coli O antigen by indirect hemagglutination for detection of acute pyelonephritis was investigated in sequences of sera from 94 girls with their first known attack of symptomatic urinary tract infection. Using O antigen from the infecting bacterial strain or a standard strain increased antibody titres were found among pyelonephritis patients in 89% in unreduced sera (mainly 19 S antibodies) and in 81% in reduced sera (mainly 7 S antibodies) compared to a control material of 643 healthy children. Significant changes of these antibody titres occurred in 74 and 39% respectively. Analysis of maximal titres as well as titre changes in both unreduced and reduced sera showed diagnostically significant changes in two or more of these four parameters in 89% of patients with pyelonephritis. In contrast one parameter with such changes was found among the cystitis patients in only 5%. Employing a pool of 8 common O antigens, increased antibody titres were detected in 76% of patients with pyelonephritis. A larger pool of 68 antigens was no better and had a low capacity to detect 7 S antibodies. Optimal time for blood sampling was 2 to 3 weeks after onset of symptoms."}
{"id": "PMID:1090110", "title": "Ribonucleic acid synthesis after adsorption of the bacteriophage lambda on Escherichia coli minicells.", "content": "Lambda bacteriophage adsorbs on the chromosome-less small bodies (minicells) produced by aberrant cell division of Escherichia coli P678-54. The plasmid-less minicells, as well as the minicells harbouring the colicinogenic factor E1, reveal a considerable inhibition of total RNA synthesis after phage adsorption. Both kinds of minicells synthesize RNA hybridizable to lambda DNA, but in the plasmid-harbouring minicells lambda RNA synthesis is much more intensive.", "contents": "Ribonucleic acid synthesis after adsorption of the bacteriophage lambda on Escherichia coli minicells. Lambda bacteriophage adsorbs on the chromosome-less small bodies (minicells) produced by aberrant cell division of Escherichia coli P678-54. The plasmid-less minicells, as well as the minicells harbouring the colicinogenic factor E1, reveal a considerable inhibition of total RNA synthesis after phage adsorption. Both kinds of minicells synthesize RNA hybridizable to lambda DNA, but in the plasmid-harbouring minicells lambda RNA synthesis is much more intensive."}
{"id": "PMID:1090111", "title": "A search for Saccharomyces cerevisiae mutants with an increased sensitivity to nitrous acid.", "content": "Six strains with an increased nitrous acid sensitivity were isolated (Fig. 1). The putative HNO2-sensitive mutants, as well as the parental strain 55R5 behaved abnormally in crosses, so that studies on the segregation of the sensitivity were difficult and unreliable. During 1.5 years the oversensitivity of the mutants gradually decreased to disappear completely (Tab. V). The differences in HNO2 sensitivity between respiratory-sufficient and cytoplasmic respiratory-deficient strains (Tab. I), as well as between different respiratory-sufficient strains (Tab. II-IV) are analysed.", "contents": "A search for Saccharomyces cerevisiae mutants with an increased sensitivity to nitrous acid. Six strains with an increased nitrous acid sensitivity were isolated (Fig. 1). The putative HNO2-sensitive mutants, as well as the parental strain 55R5 behaved abnormally in crosses, so that studies on the segregation of the sensitivity were difficult and unreliable. During 1.5 years the oversensitivity of the mutants gradually decreased to disappear completely (Tab. V). The differences in HNO2 sensitivity between respiratory-sufficient and cytoplasmic respiratory-deficient strains (Tab. I), as well as between different respiratory-sufficient strains (Tab. II-IV) are analysed."}
{"id": "PMID:1090112", "title": "Observations on the serologic changes in the recombinants in Shigella flexneri Serotype 4a.", "content": "Studies on the antigenic composition of 84 diagnostic strains of S. flexneri serotype 4a and their Lac+ recombinants from a cross with E. coli HfrC were carried out. Considerable differentiation of agglutination reaction was found both in original material and in recombinants. An interesting group included recombinants which completely lost their capability to agglutinate with sera specific for type and group antigens and preserved only the capability to agglutinate in the polyvalent serum of S. flexneri subgroup. One of them (recombinant 140 Lac+) had been subject to detailed serological analysis and was shown to acquire as a result of recombination a new antigen appearing neither in any flexneri subgroup serotype, nor in dysenteriae and boydii subgroups. This antigen is of thermostable character.", "contents": "Observations on the serologic changes in the recombinants in Shigella flexneri Serotype 4a. Studies on the antigenic composition of 84 diagnostic strains of S. flexneri serotype 4a and their Lac+ recombinants from a cross with E. coli HfrC were carried out. Considerable differentiation of agglutination reaction was found both in original material and in recombinants. An interesting group included recombinants which completely lost their capability to agglutinate with sera specific for type and group antigens and preserved only the capability to agglutinate in the polyvalent serum of S. flexneri subgroup. One of them (recombinant 140 Lac+) had been subject to detailed serological analysis and was shown to acquire as a result of recombination a new antigen appearing neither in any flexneri subgroup serotype, nor in dysenteriae and boydii subgroups. This antigen is of thermostable character."}
{"id": "PMID:1090113", "title": "Effects of disintegration of incorporated 35S on mutation frequency.", "content": "The reverse mutation to the wild type of lambda sus 9 and T4 AM B17 bacteriophages, grown in bacteria prelabeled with 35S and stored at--196 degrees, was studied. No mutagenic effect of 35S was observed in both, lambda and T4 bacteriophages. It is suggested that there is no influence of 35S disintegrations on the properties of DNA polymerase to which the mutagenic activity could be attributed.", "contents": "Effects of disintegration of incorporated 35S on mutation frequency. The reverse mutation to the wild type of lambda sus 9 and T4 AM B17 bacteriophages, grown in bacteria prelabeled with 35S and stored at--196 degrees, was studied. No mutagenic effect of 35S was observed in both, lambda and T4 bacteriophages. It is suggested that there is no influence of 35S disintegrations on the properties of DNA polymerase to which the mutagenic activity could be attributed."}
{"id": "PMID:1090114", "title": "Comparison of the mutagenic effects of 35S decay in Escherichia coli strains WP-2 and WP-2S.", "content": "Comparison was made of the lethal and mutagenic efficiency of 35S yields 35Cl transmutation of incorporated 35S in cells of Escherichia coli strain WP-2 and WP-2S (UV-sensitive). Bacteria were stored at minus 196 degrees. 35S yields 35Cl transmutation induced a higher lethal effect in strain WP-2 than in the UV-sensitive strain WP-2S. Reversions try yields try+ were induced with an approximately similar efficiency in both strains compared.", "contents": "Comparison of the mutagenic effects of 35S decay in Escherichia coli strains WP-2 and WP-2S. Comparison was made of the lethal and mutagenic efficiency of 35S yields 35Cl transmutation of incorporated 35S in cells of Escherichia coli strain WP-2 and WP-2S (UV-sensitive). Bacteria were stored at minus 196 degrees. 35S yields 35Cl transmutation induced a higher lethal effect in strain WP-2 than in the UV-sensitive strain WP-2S. Reversions try yields try+ were induced with an approximately similar efficiency in both strains compared."}
{"id": "PMID:1090132", "title": "The supra-additive natriuretic effect addition of bendroflumethiazide and bumetanide in congestive heart failure. Permutation trial tests in patients in long-term treatment with bumetanide.", "content": "The additive natriuretic effect of a single dose of bendroflumethiazide, 5 mg., has been studied in patients with advanced congestive heart failure in long-term treatment with bumetanide, 4 mg., daily. Three permutation trial tests were performed including six patients each. In the first trial, the response to supplementary bendroflumethiazide, 5 mg., was definitely superior to that of additional bumetanide, 4 mg., in terms of renal output of sodium, chloride, potassium, water, and osmolar clearance. In the second trial, a similar pattern was found in patients receiving a combination of bumetanide, 4 mg., and spironolactone, 100 mg., daily. The third trial compared the effects of bendroflumethiazide, 5 mg., plus bumetanide, 4 mg.; of bendroflumethiazide, 5 mg.; and of bumetanide, 4 mg. In terms of natriuresis and chloruresis, the response to the combination of two drugs was significantly larger than the sum of the effects of other treatments. It is concluded that the combined effects of the drugs represent a supra-additive effect addition for sodium and chloride. A tentative explanation of the mechanism of interaction in terms of inhibition of renal tubular supplementary spironolactone, involve a tendency to development of hypokalemia, hypochloremia, and alkalosis, it is recommended that supplementary use of bendroflumethiazide in this setting is combined with the administration of potassium chloride or potassium-saving diuretics.", "contents": "The supra-additive natriuretic effect addition of bendroflumethiazide and bumetanide in congestive heart failure. Permutation trial tests in patients in long-term treatment with bumetanide. The additive natriuretic effect of a single dose of bendroflumethiazide, 5 mg., has been studied in patients with advanced congestive heart failure in long-term treatment with bumetanide, 4 mg., daily. Three permutation trial tests were performed including six patients each. In the first trial, the response to supplementary bendroflumethiazide, 5 mg., was definitely superior to that of additional bumetanide, 4 mg., in terms of renal output of sodium, chloride, potassium, water, and osmolar clearance. In the second trial, a similar pattern was found in patients receiving a combination of bumetanide, 4 mg., and spironolactone, 100 mg., daily. The third trial compared the effects of bendroflumethiazide, 5 mg., plus bumetanide, 4 mg.; of bendroflumethiazide, 5 mg.; and of bumetanide, 4 mg. In terms of natriuresis and chloruresis, the response to the combination of two drugs was significantly larger than the sum of the effects of other treatments. It is concluded that the combined effects of the drugs represent a supra-additive effect addition for sodium and chloride. A tentative explanation of the mechanism of interaction in terms of inhibition of renal tubular supplementary spironolactone, involve a tendency to development of hypokalemia, hypochloremia, and alkalosis, it is recommended that supplementary use of bendroflumethiazide in this setting is combined with the administration of potassium chloride or potassium-saving diuretics."}
{"id": "PMID:1090133", "title": "Inotropic effects of tolbutamide in man.", "content": "The hemodynamic responses of normal subjects to intravenous injections of tolbutamide, 250 mg., and 1,000 mg., were assessed by measurements of serial systolic time intervals. Analysis of results, compared to saline control, revealed evidence of minor inotropic effects during the period five to 10 minutes after infusion. Small but statistically significant (p less than 0.05) decreases in pre-ejection phase and electromechanical systole were noted. The time response of these changes did not correlate with dose of blood level of tolbutamide, and appeared to coincide with peak insulin levels. No inotropic or chronotropic effects were seen during the first four minutes after infusion, suggesting that the myocardial adenyl cyclase-stimulating properties of the drug, previously demonstrated in vitro, are not significant in intact man. The minor late inotropic effects are of doubtful clinical significance, and cannot be invoked to explain the reported increased cardiovascular mortality of patients treated with tolbutamide.", "contents": "Inotropic effects of tolbutamide in man. The hemodynamic responses of normal subjects to intravenous injections of tolbutamide, 250 mg., and 1,000 mg., were assessed by measurements of serial systolic time intervals. Analysis of results, compared to saline control, revealed evidence of minor inotropic effects during the period five to 10 minutes after infusion. Small but statistically significant (p less than 0.05) decreases in pre-ejection phase and electromechanical systole were noted. The time response of these changes did not correlate with dose of blood level of tolbutamide, and appeared to coincide with peak insulin levels. No inotropic or chronotropic effects were seen during the first four minutes after infusion, suggesting that the myocardial adenyl cyclase-stimulating properties of the drug, previously demonstrated in vitro, are not significant in intact man. The minor late inotropic effects are of doubtful clinical significance, and cannot be invoked to explain the reported increased cardiovascular mortality of patients treated with tolbutamide."}
{"id": "PMID:1090134", "title": "The use of echocardiography to measure isometric contraction time.", "content": "The current methods for estimating isometric contraction time were discussed. Ultrasonically derived isometric contraction time, using external carotid pulse tracing, phonocardiogram, and the B-point of the mitral echogram was also measured. Recordings were performed in 10 normal subjects, and 15 patients. Hypertrophic cardiomyopathy (5), congestive cardiomyopathy (6), and ischemic heart disease (4). In 11 patients, the results were correlated with the internal isometric contraction time. The ultrasound isometric contraction time showed good correlation with the internal isometric contraction time (r equals 0.92, P less than 0.01). The external isometric contraction time showed less correlation with the internal isometric contraction time and was significantly shorter (P less than 0.01). The ultrasound isometric contraction time showed a superior discriminating value to the external isometric contraction time for differentiation the normal subjects from the patients' group.", "contents": "The use of echocardiography to measure isometric contraction time. The current methods for estimating isometric contraction time were discussed. Ultrasonically derived isometric contraction time, using external carotid pulse tracing, phonocardiogram, and the B-point of the mitral echogram was also measured. Recordings were performed in 10 normal subjects, and 15 patients. Hypertrophic cardiomyopathy (5), congestive cardiomyopathy (6), and ischemic heart disease (4). In 11 patients, the results were correlated with the internal isometric contraction time. The ultrasound isometric contraction time showed good correlation with the internal isometric contraction time (r equals 0.92, P less than 0.01). The external isometric contraction time showed less correlation with the internal isometric contraction time and was significantly shorter (P less than 0.01). The ultrasound isometric contraction time showed a superior discriminating value to the external isometric contraction time for differentiation the normal subjects from the patients' group."}
{"id": "PMID:1090135", "title": "Relationship of pulmonary artery diastolic and pulmonary artery wedge pressures in mitral stenosis.", "content": "Resting and exercise hemodynamic studies were performed in 22 patients with mitral stenosis (14 men and 19 women; average age, 25 years) in normal sinus rhythm with normal pulmonary vascular resistances. A normal pulmonary vascular resistance was assumed when the resting pressure gradient between the pulmonary artery diastolic and mean pulmonary artery wedge pressures was 5 mm. Hg or less. A satisfactory correlation existed between the pulmonary artery wedge and pulmonary artery diastolic pressures at rest (r equals 0.9017) and during exercise (r equals 0.8670). A method of predicting pulmonary artery wedge pressure from pulmonary artery diastolic pressure during exercise was formulated. The correlation between the predicted and measured exercise pulmonary artery wedge pressures was very close (r equals 0.9561). It is suggested that during exercise the pulmonary artery diastolic pressure can be modified as above and substituted for mean pulmonary artery wedge pressure if the resting gradient between pulmonary artery wedge and pulmonary artery diastolic pressure is known.", "contents": "Relationship of pulmonary artery diastolic and pulmonary artery wedge pressures in mitral stenosis. Resting and exercise hemodynamic studies were performed in 22 patients with mitral stenosis (14 men and 19 women; average age, 25 years) in normal sinus rhythm with normal pulmonary vascular resistances. A normal pulmonary vascular resistance was assumed when the resting pressure gradient between the pulmonary artery diastolic and mean pulmonary artery wedge pressures was 5 mm. Hg or less. A satisfactory correlation existed between the pulmonary artery wedge and pulmonary artery diastolic pressures at rest (r equals 0.9017) and during exercise (r equals 0.8670). A method of predicting pulmonary artery wedge pressure from pulmonary artery diastolic pressure during exercise was formulated. The correlation between the predicted and measured exercise pulmonary artery wedge pressures was very close (r equals 0.9561). It is suggested that during exercise the pulmonary artery diastolic pressure can be modified as above and substituted for mean pulmonary artery wedge pressure if the resting gradient between pulmonary artery wedge and pulmonary artery diastolic pressure is known."}
{"id": "PMID:1090136", "title": "Measurement of right ventricular volumes using 131I-MAA.", "content": "A method has been presented for determining the right ventricular residual ratio, that is, the ratio of the end-systolic volume to the end-diastolic volume during each cardiac cycle. 131I-MAA was injected as a bolus into the right ventricle, and the ratio of isotope remaining in the chamber during the succeeding cardiac cycles was determined with a collimated scintillation counter placed over the right ventricle. Since the counter detected the radioactivity from the entire right ventricular cavity, potential errors from incomplete mixing were minimized. The washout curve from the ventricle was distorted somewhat by the accumulation of isotope in intervening lung tissue. This distortion was eliminated by subtracting the build-up curve of radioactivity in the lung recorded simultaneously with a second scintillation counter positioned over the lateral chest wall. In 14 dogs anesthetized with chloralose, the right ventricular residual ratio was relatively constant at 40.4 plus or minus 3.1 per cent. Duplicate measurements differed by less than 3 per cent indicating the good reproducibility of the method. Right ventricular stroke volume was determined from cardiac output (dye dilution) and heart rate. With this and the simultaneously determined residual ratio (131I-MAA), end-diastolic volume could be calculated. Stroke volume and stroke work were highly correlated with end-diastolic volume, in keeping with the Frank-Starling mechanism.", "contents": "Measurement of right ventricular volumes using 131I-MAA. A method has been presented for determining the right ventricular residual ratio, that is, the ratio of the end-systolic volume to the end-diastolic volume during each cardiac cycle. 131I-MAA was injected as a bolus into the right ventricle, and the ratio of isotope remaining in the chamber during the succeeding cardiac cycles was determined with a collimated scintillation counter placed over the right ventricle. Since the counter detected the radioactivity from the entire right ventricular cavity, potential errors from incomplete mixing were minimized. The washout curve from the ventricle was distorted somewhat by the accumulation of isotope in intervening lung tissue. This distortion was eliminated by subtracting the build-up curve of radioactivity in the lung recorded simultaneously with a second scintillation counter positioned over the lateral chest wall. In 14 dogs anesthetized with chloralose, the right ventricular residual ratio was relatively constant at 40.4 plus or minus 3.1 per cent. Duplicate measurements differed by less than 3 per cent indicating the good reproducibility of the method. Right ventricular stroke volume was determined from cardiac output (dye dilution) and heart rate. With this and the simultaneously determined residual ratio (131I-MAA), end-diastolic volume could be calculated. Stroke volume and stroke work were highly correlated with end-diastolic volume, in keeping with the Frank-Starling mechanism."}
{"id": "PMID:1090137", "title": "Direct and reflex cardiac bradydysrhythmias from small vagal nerve stiumaltions.", "content": "Alterations in cardiac pacemaker location, its rate of discharge, and A-V conduction patterns were induced in anesthetized adult dogs by electrical stimulation of the thoracic vagi and their small cardiac branches before and after cervical vagotomy. Electrical activity from small, contiguous bipolar silver electrodes was amplified and recorded by an optical oscillograph. The electrodes were located over the SA node, the three internodal pathways, the left atrium, and ventricular epicardium. A hoffman-type plaque electrode was placed over the A-V node to record a His bundle electrogram simultaneously with a Lead II electrocardiogram. Electrical stimulation of the intact left recurrent laryngeal nerve and its cardiac branches before and after vagotomy induced both direct and reflex effects on SA nodal cycle length. Efferent dromotropic effects on the A-V node varied from first- to third-degree heart block during stimulation of individual left recurrent cardiac branches. Stimulation of the right recurrent cardiac nerve induced atrial bradycardia with heart block above the His bundle. Stimulation of individual right vagal branches near the heart induced bradycardia, cardiac asystole, shifts in atrial pacemaker location, or activation of His pacemakers. Establishment of the His rhythm probably indicates selective inhibition of supraventricular but not of the His bundle. Asystole and His rhythms induced during stimulation of the more caudal branches of the right cardiac vagal nerves were generally reflexly mediated and were abolished by cervical vagotomy.", "contents": "Direct and reflex cardiac bradydysrhythmias from small vagal nerve stiumaltions. Alterations in cardiac pacemaker location, its rate of discharge, and A-V conduction patterns were induced in anesthetized adult dogs by electrical stimulation of the thoracic vagi and their small cardiac branches before and after cervical vagotomy. Electrical activity from small, contiguous bipolar silver electrodes was amplified and recorded by an optical oscillograph. The electrodes were located over the SA node, the three internodal pathways, the left atrium, and ventricular epicardium. A hoffman-type plaque electrode was placed over the A-V node to record a His bundle electrogram simultaneously with a Lead II electrocardiogram. Electrical stimulation of the intact left recurrent laryngeal nerve and its cardiac branches before and after vagotomy induced both direct and reflex effects on SA nodal cycle length. Efferent dromotropic effects on the A-V node varied from first- to third-degree heart block during stimulation of individual left recurrent cardiac branches. Stimulation of the right recurrent cardiac nerve induced atrial bradycardia with heart block above the His bundle. Stimulation of individual right vagal branches near the heart induced bradycardia, cardiac asystole, shifts in atrial pacemaker location, or activation of His pacemakers. Establishment of the His rhythm probably indicates selective inhibition of supraventricular but not of the His bundle. Asystole and His rhythms induced during stimulation of the more caudal branches of the right cardiac vagal nerves were generally reflexly mediated and were abolished by cervical vagotomy."}
{"id": "PMID:1090141", "title": "Myocardial imaging with indium- 113m- and technetium-99m-macroaggregated albumin. New procedure for identification of stress-induced regional ischemia.", "content": "Regional coronary blood flow distribution was studied by myocardial imaging after intracoronary injection of technetium-99m- and indium-113m-macroaggregated albumin at rest and during coronary hyperemia induced by intracoronary injection of Hypaque-M, 75 percent. The four- to five-fold increase in coronary flow after injection of radiographic contrast material was similar in magnitude to that occurring with maximal exercise stress. Experimentally, resting coronary blood flow and regional distribution of radioactive particles remains normal in spite of coronary stenosis of up to 85 percent. Less severe stenosis causes flow and distribution abnormalities only during periods of increased flow, and the degree of maldistribution is directly related to the physiologic severity of the stenosis. Of 49 patients with suspected coronary artery disease, 10 had no significant lesions by coronary arteriography and all had normal images at rest and during coronary hyperemia. Thirty-seven of 39 patients with significant obstructive coronary artery disease had abnormal images at rest or during contrast agent-induced hyperemia, or both. Patients with significant coronary artery disease without previous infarction usually demonstrated abnormalities in flow distribution only during coronary hyperemia. Patients with previous infarction demonstrated resting perfusion abnormalities that often became more abnormal during hyperemia. This technique provides a new method for assessing the physiologic effects of coronary stenosis in conjuntion with coronary arteriography.", "contents": "Myocardial imaging with indium- 113m- and technetium-99m-macroaggregated albumin. New procedure for identification of stress-induced regional ischemia. Regional coronary blood flow distribution was studied by myocardial imaging after intracoronary injection of technetium-99m- and indium-113m-macroaggregated albumin at rest and during coronary hyperemia induced by intracoronary injection of Hypaque-M, 75 percent. The four- to five-fold increase in coronary flow after injection of radiographic contrast material was similar in magnitude to that occurring with maximal exercise stress. Experimentally, resting coronary blood flow and regional distribution of radioactive particles remains normal in spite of coronary stenosis of up to 85 percent. Less severe stenosis causes flow and distribution abnormalities only during periods of increased flow, and the degree of maldistribution is directly related to the physiologic severity of the stenosis. Of 49 patients with suspected coronary artery disease, 10 had no significant lesions by coronary arteriography and all had normal images at rest and during coronary hyperemia. Thirty-seven of 39 patients with significant obstructive coronary artery disease had abnormal images at rest or during contrast agent-induced hyperemia, or both. Patients with significant coronary artery disease without previous infarction usually demonstrated abnormalities in flow distribution only during coronary hyperemia. Patients with previous infarction demonstrated resting perfusion abnormalities that often became more abnormal during hyperemia. This technique provides a new method for assessing the physiologic effects of coronary stenosis in conjuntion with coronary arteriography."}
{"id": "PMID:1090142", "title": "Scanning electron microscopy of cardiac endothelium of the dog.", "content": "Tissue obtained from young dogs was fixed in buffered aldehydes by vascular perfusion or direct immersion. Selected hearts were maintained in modified mammalian Ringer's solution for three to five minutes prior to fixation. The chambers of the heart and related valves were exposed by dissection and prepared by routine techniques for observation by scanning electron microscopy (SEM). Subsequent to SEM studies, selected specimens were embedded in Epon 812 and sectioned for transmission electron microscopy (TEM). The cardiac endothelium, when fixed immediately in buffered aldehydes, presents an essentially invariable surface throughout the interior of the heart. The predominant nuclear bulges and attenuated peripheral plasmatemma are consistently smooth, with occasional marginal ruffles, scattered microvilli and small blebs. Apart from the higher population of nuclear bulges on valvular surfaces, local variations in SEM of endocardium occur in response to the various stages of systole and diastole encountered. These physiological changes do not produce microappendages. Immersion for three to five minutes in (Chenoweth's) Ringer's solution, prior to fixation, produces a substantial population of microappendages. The cellular surface acquires a swirled appearance erupting in microvilli, blebs and ruffles. These exhibit considerable pleomorphism. There is great lability of the endocardial surface in response to a classic \"holding solution\" widely used in preparatory techniques. In preparing soft tissues for SEM caution must be used if physiological \"holding solutions\" are used prior to fixation.", "contents": "Scanning electron microscopy of cardiac endothelium of the dog. Tissue obtained from young dogs was fixed in buffered aldehydes by vascular perfusion or direct immersion. Selected hearts were maintained in modified mammalian Ringer's solution for three to five minutes prior to fixation. The chambers of the heart and related valves were exposed by dissection and prepared by routine techniques for observation by scanning electron microscopy (SEM). Subsequent to SEM studies, selected specimens were embedded in Epon 812 and sectioned for transmission electron microscopy (TEM). The cardiac endothelium, when fixed immediately in buffered aldehydes, presents an essentially invariable surface throughout the interior of the heart. The predominant nuclear bulges and attenuated peripheral plasmatemma are consistently smooth, with occasional marginal ruffles, scattered microvilli and small blebs. Apart from the higher population of nuclear bulges on valvular surfaces, local variations in SEM of endocardium occur in response to the various stages of systole and diastole encountered. These physiological changes do not produce microappendages. Immersion for three to five minutes in (Chenoweth's) Ringer's solution, prior to fixation, produces a substantial population of microappendages. The cellular surface acquires a swirled appearance erupting in microvilli, blebs and ruffles. These exhibit considerable pleomorphism. There is great lability of the endocardial surface in response to a classic \"holding solution\" widely used in preparatory techniques. In preparing soft tissues for SEM caution must be used if physiological \"holding solutions\" are used prior to fixation."}
{"id": "PMID:1090143", "title": "Embedding brain specimens in transparent plastic.", "content": "A simple, reliable and practical technic for permanently embedding brain specimens in plastic is discussed. The anatomic details, including the natural colors, are so well preserved that they give a real picture of the morphologic details, such as preservation of anatomic landmarks and pathologic alterations. The specimen can be observed from different angles, enhancing the student's opportunity for visual examination. Clearing of the specimen with visualization of the vascular tree after injection with opaque dyes may be accomplished. The procedure is recommended for museums and laboratory teaching.", "contents": "Embedding brain specimens in transparent plastic. A simple, reliable and practical technic for permanently embedding brain specimens in plastic is discussed. The anatomic details, including the natural colors, are so well preserved that they give a real picture of the morphologic details, such as preservation of anatomic landmarks and pathologic alterations. The specimen can be observed from different angles, enhancing the student's opportunity for visual examination. Clearing of the specimen with visualization of the vascular tree after injection with opaque dyes may be accomplished. The procedure is recommended for museums and laboratory teaching."}
{"id": "PMID:1090144", "title": "IgD deposition in glomerulonephritis. An immunopathologic study.", "content": "Diffuse deposits of IgD were found in glomerular basement membranes in eight of 263 consecutive renal biopsies by the direct immunofluorescent technic. In five biopsies a granular staining pattern was found and in three a linear pattern was found. IgG and beta1C were present in glomeruli of all eight patients, and IgM in four. Two patients had elevated titers of antithyroglobulin antibodies in the serum, and one patient had anti-glomerular basement membrane antibodies of the IgG class in both serum and renal eluate. This study suggests that IgD may play a role in immunologically mediated glomerular disease. (Key words: IgD; Glomerular deposits; Nephritis.)", "contents": "IgD deposition in glomerulonephritis. An immunopathologic study. Diffuse deposits of IgD were found in glomerular basement membranes in eight of 263 consecutive renal biopsies by the direct immunofluorescent technic. In five biopsies a granular staining pattern was found and in three a linear pattern was found. IgG and beta1C were present in glomeruli of all eight patients, and IgM in four. Two patients had elevated titers of antithyroglobulin antibodies in the serum, and one patient had anti-glomerular basement membrane antibodies of the IgG class in both serum and renal eluate. This study suggests that IgD may play a role in immunologically mediated glomerular disease. (Key words: IgD; Glomerular deposits; Nephritis.)"}
{"id": "PMID:1090145", "title": "Acute glomerulonephritis. Immunofluorescent and electron-microscopic observations in sporadic cases.", "content": "The authors studied by immunofluorescent and electron microscopy renal biopsy specimens from 29 patients with various glomerular diseases. Poststreptococcal glomerulonephritis was characterized by the presence of complement (beta1C) in the mesangium and/or on the basement membrane in all cases. Immunoglobulin G (IgG) was also present in less than half of the cases in the same distribution. Electron microscopy, carried out in six cases, revealed no uniform ultrastructural change: minimal subepi helial deposits were observed in three cases, intramembranous deposits were seen in two cases, and the basement membrane was normal in one case. Glomerular abnormalities during the courses of some systemic diseases were similar. Mild renal involvement was characterized by only beta1C deposition. This finding raises the question whether a mechanism other than or in addition to that involving immune complexes is operative in the pathogenesis of acute glomerulonephritis. There is circumstantial experimental evidence that an alternate pathway of complement activation and deposition may be operative in acute glomerulonephritis.", "contents": "Acute glomerulonephritis. Immunofluorescent and electron-microscopic observations in sporadic cases. The authors studied by immunofluorescent and electron microscopy renal biopsy specimens from 29 patients with various glomerular diseases. Poststreptococcal glomerulonephritis was characterized by the presence of complement (beta1C) in the mesangium and/or on the basement membrane in all cases. Immunoglobulin G (IgG) was also present in less than half of the cases in the same distribution. Electron microscopy, carried out in six cases, revealed no uniform ultrastructural change: minimal subepi helial deposits were observed in three cases, intramembranous deposits were seen in two cases, and the basement membrane was normal in one case. Glomerular abnormalities during the courses of some systemic diseases were similar. Mild renal involvement was characterized by only beta1C deposition. This finding raises the question whether a mechanism other than or in addition to that involving immune complexes is operative in the pathogenesis of acute glomerulonephritis. There is circumstantial experimental evidence that an alternate pathway of complement activation and deposition may be operative in acute glomerulonephritis."}
{"id": "PMID:1090146", "title": "Immunofluorescence of soft-tissue tumors with anti-smooth-muscle and anti-skeletal-muscle antibodies.", "content": "One hundred thirteen soft-tissue tumors were studied by immunofluorescence using anti-smooth-muscle (SMA) and anti-skeletal-muscle antibodies (MGA). Of the classic leiomyomas examined, 94% manifested bright fluorescence with SMA, whereas cellular leiomyomas and leiomyosarcomas failed to stain. Of the rhabdomyosarcomas studied, 21% showed fluorescent cross-striations or fluorescent granules after incubation with MGA. None of 48 control tumors fluoresced with either antibody. No tumor vessel seen showed smooth-muscle fluorescence in its walls. Although of little diagnostic value, immunofluorescence utilizing specific human antibodies is a valuable tool with which to study some of the antigenic components of these neoplasms.", "contents": "Immunofluorescence of soft-tissue tumors with anti-smooth-muscle and anti-skeletal-muscle antibodies. One hundred thirteen soft-tissue tumors were studied by immunofluorescence using anti-smooth-muscle (SMA) and anti-skeletal-muscle antibodies (MGA). Of the classic leiomyomas examined, 94% manifested bright fluorescence with SMA, whereas cellular leiomyomas and leiomyosarcomas failed to stain. Of the rhabdomyosarcomas studied, 21% showed fluorescent cross-striations or fluorescent granules after incubation with MGA. None of 48 control tumors fluoresced with either antibody. No tumor vessel seen showed smooth-muscle fluorescence in its walls. Although of little diagnostic value, immunofluorescence utilizing specific human antibodies is a valuable tool with which to study some of the antigenic components of these neoplasms."}
{"id": "PMID:1090147", "title": "Pulmonary granulomatous pneumocystosis following renal transplantation. Report of a case.", "content": "Pulmonary granulomas of sarcoid type in a patient who was on long-term immunosuppressive therapy following renal transplantation are described. Pneumocystis carinii was identified within the lesions. This is believed to be the first description of this type of reaction to Pneumocystis in the adult patient on immunosuppressive therapy.", "contents": "Pulmonary granulomatous pneumocystosis following renal transplantation. Report of a case. Pulmonary granulomas of sarcoid type in a patient who was on long-term immunosuppressive therapy following renal transplantation are described. Pneumocystis carinii was identified within the lesions. This is believed to be the first description of this type of reaction to Pneumocystis in the adult patient on immunosuppressive therapy."}
{"id": "PMID:1090148", "title": "Selective hypogammaglobulinemia with persistence of IgE, malabsorption and a nutritionally dependent, reversible defect in cell-mediated immunity.", "content": "A 46 year old man presented with selective hypogammaglobulinemia, malabsorption and long-standing secondary malnutrition. Although the patient had essentially unmeasurable levels of immunoglobulins G (IgG), M (IgA), he had normal levels of immunoglobulin E (IgE). He was found to be anergic when tested for the delayed cutaneous hypersensitivity reaction. Evaluation of his cell-mediated immunity in vivo and in vitro suggested one discrete lesion, a defective production of the lymphocyte mediator macrophage migration inhibitory factor. With improved nutrition the patient \"repaired\" this defect in the \"efferent\" limb of cellular immunity and was no longer anergic.", "contents": "Selective hypogammaglobulinemia with persistence of IgE, malabsorption and a nutritionally dependent, reversible defect in cell-mediated immunity. A 46 year old man presented with selective hypogammaglobulinemia, malabsorption and long-standing secondary malnutrition. Although the patient had essentially unmeasurable levels of immunoglobulins G (IgG), M (IgA), he had normal levels of immunoglobulin E (IgE). He was found to be anergic when tested for the delayed cutaneous hypersensitivity reaction. Evaluation of his cell-mediated immunity in vivo and in vitro suggested one discrete lesion, a defective production of the lymphocyte mediator macrophage migration inhibitory factor. With improved nutrition the patient \"repaired\" this defect in the \"efferent\" limb of cellular immunity and was no longer anergic."}
{"id": "PMID:1090149", "title": "Renal biogenesis of erythropoietin.", "content": "The widespread and ever expanding use of dialysis in the maintenance of patients with chronic renal disease has added an urgency to the study of the biogenesis of erythropoietin. It seems almost certain that erythropoietin could ameliorate, if not eliminate, the anemia of uremia, but unfortunately, erythropoietin is still not available in therapeutic quantities. Initially, erythropietin was though to be produced by the kidneys\" but then the attention became directed at the liver. It was proposed that erythropoietin was produced there as an inactive precursor and that the kidney only acted as an oxygen sensor and as a producer of an erythropoietin-activating enzyme. Recent studies summarized here show that an isolated perfused kidney in the absence of any extrarenal substrate or precursor can synthesize erythropoietin. Consequently, it appears almost certain that the kidney is the endocrine organ of origin of erythropoietin. Further studies suggest that erythropoietin formation involves a phase of oxygen sensing and programming and a phase of synthesis. These phases probably occur in the same cell, and the renal cortex appears to be the most likely location for such cells. The current inability to extract erythropoietin from kidney homogenates is discussed but, unfortunately, not adequately explained.", "contents": "Renal biogenesis of erythropoietin. The widespread and ever expanding use of dialysis in the maintenance of patients with chronic renal disease has added an urgency to the study of the biogenesis of erythropoietin. It seems almost certain that erythropoietin could ameliorate, if not eliminate, the anemia of uremia, but unfortunately, erythropoietin is still not available in therapeutic quantities. Initially, erythropietin was though to be produced by the kidneys\" but then the attention became directed at the liver. It was proposed that erythropoietin was produced there as an inactive precursor and that the kidney only acted as an oxygen sensor and as a producer of an erythropoietin-activating enzyme. Recent studies summarized here show that an isolated perfused kidney in the absence of any extrarenal substrate or precursor can synthesize erythropoietin. Consequently, it appears almost certain that the kidney is the endocrine organ of origin of erythropoietin. Further studies suggest that erythropoietin formation involves a phase of oxygen sensing and programming and a phase of synthesis. These phases probably occur in the same cell, and the renal cortex appears to be the most likely location for such cells. The current inability to extract erythropoietin from kidney homogenates is discussed but, unfortunately, not adequately explained."}
{"id": "PMID:1090150", "title": "Calcium metabolism in renal failure.", "content": "Osteodystrophy is almost universally present in chronic renal failure. Mild, but detectable, abnormalities--especially in parathyroid hormone (PTH) secretion--occur even when the glomerular filtration rate is greater than 30 cc/min. Osteomalacia is common in areas in which vitamin D intake and exposure to sunlight are minimal; when these factors are plentiful, osteitis fibrosa predominates. Osteoporosis is seen with increasing frequency in hemodialyzed patients. Nonosseous complications of secondary hyper-parathyroidism include hypercalcemia, metastatic calcification and pruritus. The most important factor in the medical therapy of osteodystrophy is control of serum phosphate levels. Next, a positive calcium balance must be provided either by giving vitamin D as dihyrdotachysterol, raising dialysate calcium or administering calcium orally. Parathyroidectomy is sometimes indicated, especially when the patients are transplant candidates and manifest hypercalcemia. Whether or not transplant is contemplated, patients with persistently high calcium-phosphate products, severe metastatic calcification or rapidly progressive osteodystrophy should be considered for parathyroidectomy. Newer, experimental vitamin D preparations, such as 1,25-dihydroxycholecalciferol or 1-alpha-hydroxycholecalciferol, should improve the managemet of patients with renal osteodystrophy and decrease the need for parathyroidectomies.", "contents": "Calcium metabolism in renal failure. Osteodystrophy is almost universally present in chronic renal failure. Mild, but detectable, abnormalities--especially in parathyroid hormone (PTH) secretion--occur even when the glomerular filtration rate is greater than 30 cc/min. Osteomalacia is common in areas in which vitamin D intake and exposure to sunlight are minimal; when these factors are plentiful, osteitis fibrosa predominates. Osteoporosis is seen with increasing frequency in hemodialyzed patients. Nonosseous complications of secondary hyper-parathyroidism include hypercalcemia, metastatic calcification and pruritus. The most important factor in the medical therapy of osteodystrophy is control of serum phosphate levels. Next, a positive calcium balance must be provided either by giving vitamin D as dihyrdotachysterol, raising dialysate calcium or administering calcium orally. Parathyroidectomy is sometimes indicated, especially when the patients are transplant candidates and manifest hypercalcemia. Whether or not transplant is contemplated, patients with persistently high calcium-phosphate products, severe metastatic calcification or rapidly progressive osteodystrophy should be considered for parathyroidectomy. Newer, experimental vitamin D preparations, such as 1,25-dihydroxycholecalciferol or 1-alpha-hydroxycholecalciferol, should improve the managemet of patients with renal osteodystrophy and decrease the need for parathyroidectomies."}
{"id": "PMID:1090151", "title": "Renal handling of low molecular weight proteins.", "content": "The renal filtration, absorption and final disposal of lysozyme (lysozyme--mol wt 14,000), insulin and growth hormone were studied to gain a better quantitative understanding of the fundamental variables involved in the renal handling of low molecular weight proteins. The glomerular barrier offers little hindrance to the filtration of lysozyme, the glomerular sieving coefficient being 0.8 plus or minus 0.1 (SD). The intrarenal route by which injected lysozyme accumulates in the kidney is via filtration and subsequent absorption (uptake) by renal tubular cells. Uptake or adsorption from the peritubular side is negligible compared to luminal uptake. Renal clearance and renal titration experiments in the intact dog and in the isolated perfused rat kidney showed that the lysozyme absorption process can be best characterized as high capacity, low affinity transport system which is directly or indirectly dependent on energy input. The final disposal of absorbed 125I-lysozyme, 125I-insulin and 125I-growth hormone was studied in the isolated perfused rat kidney by measuring the radioactivity by gel chromatography. The rate of release of radioactivity as well as its nature was dependent on the molecular species of the absorbed protein. The rate of release was higher for 125I-insulin and 125I-growth hormone and lower for 125I-lysozyme. Lysozyme absorbed from the luminal side was released to the perfusate both as intact protein molecules and as catabolic products, whereas absorbed 125I-insulin was almost entirely released to the perfusate as catabolic products. It is concluded that low molecular weight proteins are extensively filtered by the kidney, absorbed from the luminal side by renal tubular cells and released back to the circulation either as intact molecules or as catabolic products (amino acids and polypeptides). This process contributes in an important way to the plasma turn-over of low molecular weight proteins including peptides and proteins hormones.", "contents": "Renal handling of low molecular weight proteins. The renal filtration, absorption and final disposal of lysozyme (lysozyme--mol wt 14,000), insulin and growth hormone were studied to gain a better quantitative understanding of the fundamental variables involved in the renal handling of low molecular weight proteins. The glomerular barrier offers little hindrance to the filtration of lysozyme, the glomerular sieving coefficient being 0.8 plus or minus 0.1 (SD). The intrarenal route by which injected lysozyme accumulates in the kidney is via filtration and subsequent absorption (uptake) by renal tubular cells. Uptake or adsorption from the peritubular side is negligible compared to luminal uptake. Renal clearance and renal titration experiments in the intact dog and in the isolated perfused rat kidney showed that the lysozyme absorption process can be best characterized as high capacity, low affinity transport system which is directly or indirectly dependent on energy input. The final disposal of absorbed 125I-lysozyme, 125I-insulin and 125I-growth hormone was studied in the isolated perfused rat kidney by measuring the radioactivity by gel chromatography. The rate of release of radioactivity as well as its nature was dependent on the molecular species of the absorbed protein. The rate of release was higher for 125I-insulin and 125I-growth hormone and lower for 125I-lysozyme. Lysozyme absorbed from the luminal side was released to the perfusate both as intact protein molecules and as catabolic products, whereas absorbed 125I-insulin was almost entirely released to the perfusate as catabolic products. It is concluded that low molecular weight proteins are extensively filtered by the kidney, absorbed from the luminal side by renal tubular cells and released back to the circulation either as intact molecules or as catabolic products (amino acids and polypeptides). This process contributes in an important way to the plasma turn-over of low molecular weight proteins including peptides and proteins hormones."}
{"id": "PMID:1090152", "title": "Growth in children with renal failure.", "content": "Chronic renal insufficiency in childhood is often associated with growth delay. Undernutrition, osteodystrophy and diminished somatomedin levels appear to be importantly related to this growth delay. In many but not all children with renal failure and growth retardation, somatomedin levels are diminished, growth hormone levels are normal or elevated, and glucose tolerance is often abnormal. Following renal transplantation, growth restoration appears to be related to bone age, renal function, somatomedin and steroid therapy.", "contents": "Growth in children with renal failure. Chronic renal insufficiency in childhood is often associated with growth delay. Undernutrition, osteodystrophy and diminished somatomedin levels appear to be importantly related to this growth delay. In many but not all children with renal failure and growth retardation, somatomedin levels are diminished, growth hormone levels are normal or elevated, and glucose tolerance is often abnormal. Following renal transplantation, growth restoration appears to be related to bone age, renal function, somatomedin and steroid therapy."}
{"id": "PMID:1090153", "title": "Clinical effects of bilateral nephrectomy.", "content": "The effects of removal of all renal tissue on hematopoiesis, osteodystrophy, blood pressure regulation and metabolic functions are reviewed; and, the indications for, and results of, bilateral nephrectomy are discussed. Nephrectomy results in a more severe anemia in dialysis patients which is poorly responsive to androgen therapy. No differences were detected in the severity of osteodystrophy between nephric and anephric patients. However, bilateral nephrectomy can occasionally result in the acute onset of hypocalcemia. Blood pressure regulation must be accomplished in the absence of a functioning renin-angiotensin system. This is largely on the basis of volume, but changes in vascular tone may also be significant. Little is known about the metabolic consequences of nephrectomies. The effect on substances metabolized by the kidney is an area for further investigation. Kidney tissue should be preserved, if at all possible, and nephrectomy performed only for specific indications.", "contents": "Clinical effects of bilateral nephrectomy. The effects of removal of all renal tissue on hematopoiesis, osteodystrophy, blood pressure regulation and metabolic functions are reviewed; and, the indications for, and results of, bilateral nephrectomy are discussed. Nephrectomy results in a more severe anemia in dialysis patients which is poorly responsive to androgen therapy. No differences were detected in the severity of osteodystrophy between nephric and anephric patients. However, bilateral nephrectomy can occasionally result in the acute onset of hypocalcemia. Blood pressure regulation must be accomplished in the absence of a functioning renin-angiotensin system. This is largely on the basis of volume, but changes in vascular tone may also be significant. Little is known about the metabolic consequences of nephrectomies. The effect on substances metabolized by the kidney is an area for further investigation. Kidney tissue should be preserved, if at all possible, and nephrectomy performed only for specific indications."}
{"id": "PMID:1090155", "title": "Hereditary complement (C2) deficiency with dermatomyositis.", "content": "A 60 year old white man in previous good health presented with a 6 month history of progressive muscle weakness. Clinical and laboratory findings were typical of dermatomyositis. Muscle biopsy confirmed the presence of inflammatory myopathy; deposits of immunoglobulin G (IgG), immunoglobulin M (IgM) or third component of complement (C3) were not detected by immunofluorescence. No evidence was found for an associated neoplasm. An unexpected finding was the total absence of serum hemolytic complement activity. Further investigation revealed that the complement defect was attributable to a selective and total absence of the second component of complement (C2), as determined by both functional and immunoprecipitin assays. Family studies indicated that the defect was inherited in an autosomal recessive manner, as has been observed in the previously reported C2-deficient kindreds. This case demonstrates that typical muscle lesions of dermatomyositis can occur in the presence of a complement defect which would preclude activation of the classic (C1-C4-C2) complement pathway. The case is of further interest as one of a series of recently reported associations of rheumatic diseases with hereditary complement deficiencies. Study of the functional properties of the propositus' C2-deficient serum demonstrated normal generation of chemotactic activity in the presence of endotoxin or aggregated IgG, and normal or near normal bactericidal activity against Salmonella typhi O 901 and Hemophilus influenzae, type b. These findings emphasize the importance of the alternate (properdin) pathway of complement activiation in these functions.", "contents": "Hereditary complement (C2) deficiency with dermatomyositis. A 60 year old white man in previous good health presented with a 6 month history of progressive muscle weakness. Clinical and laboratory findings were typical of dermatomyositis. Muscle biopsy confirmed the presence of inflammatory myopathy; deposits of immunoglobulin G (IgG), immunoglobulin M (IgM) or third component of complement (C3) were not detected by immunofluorescence. No evidence was found for an associated neoplasm. An unexpected finding was the total absence of serum hemolytic complement activity. Further investigation revealed that the complement defect was attributable to a selective and total absence of the second component of complement (C2), as determined by both functional and immunoprecipitin assays. Family studies indicated that the defect was inherited in an autosomal recessive manner, as has been observed in the previously reported C2-deficient kindreds. This case demonstrates that typical muscle lesions of dermatomyositis can occur in the presence of a complement defect which would preclude activation of the classic (C1-C4-C2) complement pathway. The case is of further interest as one of a series of recently reported associations of rheumatic diseases with hereditary complement deficiencies. Study of the functional properties of the propositus' C2-deficient serum demonstrated normal generation of chemotactic activity in the presence of endotoxin or aggregated IgG, and normal or near normal bactericidal activity against Salmonella typhi O 901 and Hemophilus influenzae, type b. These findings emphasize the importance of the alternate (properdin) pathway of complement activiation in these functions."}
{"id": "PMID:1090157", "title": "Lymphocyte surface characteristics in malignant lymphoma.", "content": "The surface of lymphocytes obtained from fresh biopsy specimens from 41 patients with malignant lymphoma and from 30 normal subjects or patients with non-neoplastic lymphadenopathy were investigated. Immunoglobulin on the cell surface was used to identify B cells, whereas T cells were recognized by their reactivity with an antithymocyte antiserum and their ability to form rosettes with sheep erythrocytes. Normal and inflammatory lymph nodes were composed predominantly of T lymphocytes, as were nodes from 14 patients with Hodgkin's disease. Two thymomas were T cell proliferations, whereas a node from a patient with ataxia-telangiectasia was devoid of T lymphocytes. The presence of immunoglobulin on the cell surface indicated that 19 of 21 lymphocytic lymphomas were B cell proliferations, whereas the cells from 3 histiocytic lymphomas (reticulum cell sarcomas) and 1 mixed histiocytic and lymphocytic lymphoma were devoid of surface immunoglobulin. In immunoglobulin-positive tumors, one predominant heavy chain and one predominant light chain could usually be identified, thus establishing the clonal character of the neoplastic proliferation. Ten of 11 diffuse poorly differentiated lymphocytic lymphomas were composed of cells with large amounts of surface immunoglobulin, whereas only 1 of 5 diffuse well differentiated lymphocytic tumors contained such abundant surface immunoglobulin. The surface immunoglobulin data indicate the existence of at least two subspecies of B cell neoplasms. A small lymphocyte with sparse surface immunoglobulin proliferates as diffuse well differentiated lymphocytic lymphoma and chronic lymphocytic leukemia, whereas a larger lymphocyte with abundant surface immunoglobulin proliferates as diffuse poorly differentiated lymphocytic lymphoma and lymphosarcoma cell leukemia.", "contents": "Lymphocyte surface characteristics in malignant lymphoma. The surface of lymphocytes obtained from fresh biopsy specimens from 41 patients with malignant lymphoma and from 30 normal subjects or patients with non-neoplastic lymphadenopathy were investigated. Immunoglobulin on the cell surface was used to identify B cells, whereas T cells were recognized by their reactivity with an antithymocyte antiserum and their ability to form rosettes with sheep erythrocytes. Normal and inflammatory lymph nodes were composed predominantly of T lymphocytes, as were nodes from 14 patients with Hodgkin's disease. Two thymomas were T cell proliferations, whereas a node from a patient with ataxia-telangiectasia was devoid of T lymphocytes. The presence of immunoglobulin on the cell surface indicated that 19 of 21 lymphocytic lymphomas were B cell proliferations, whereas the cells from 3 histiocytic lymphomas (reticulum cell sarcomas) and 1 mixed histiocytic and lymphocytic lymphoma were devoid of surface immunoglobulin. In immunoglobulin-positive tumors, one predominant heavy chain and one predominant light chain could usually be identified, thus establishing the clonal character of the neoplastic proliferation. Ten of 11 diffuse poorly differentiated lymphocytic lymphomas were composed of cells with large amounts of surface immunoglobulin, whereas only 1 of 5 diffuse well differentiated lymphocytic tumors contained such abundant surface immunoglobulin. The surface immunoglobulin data indicate the existence of at least two subspecies of B cell neoplasms. A small lymphocyte with sparse surface immunoglobulin proliferates as diffuse well differentiated lymphocytic lymphoma and chronic lymphocytic leukemia, whereas a larger lymphocyte with abundant surface immunoglobulin proliferates as diffuse poorly differentiated lymphocytic lymphoma and lymphosarcoma cell leukemia."}
{"id": "PMID:1090158", "title": "Hodgkin's disease and acute leukemia. Report of eight cases and review of the literature.", "content": "Eight cases of Hodgkin's disease and acute leukemia are reported. An additional 74 cases of acute myelocytic leukemia or one of its variants, 11 cases of acute lymphocytic leukemia, 12 cases of chronic myelocytic leukemia and 37 cases of chronic lymphocytic leukemia associated with Hodgkin's disease are reviewed from the literature. In 3 of the 82 patients with acute myelocytic leukemia and Hodgkin's disease, the two diseases occurred simultaneously. Of the remaining 79 patients, 76 had received radiation therapy for their Hodgkin's disease and acute myelocytic leukemia had developed 1.2 to 19 years later (mean 6.5 years). Thirty-four of these patients also received antineoplastic chemotherapy. Only three patients with Hodgkin's disease were treated with multiple chemotherapy alone; in these, Hodgkin's disease developed 1.2, 1.5 and 3.2 years later. In 4 of 11 patients with acute lymphocytic leukemia and Hodgkin's disease, the two disorders occurred simultaneously. The other seven patients were all treated with radiation for their Hodgkin's disease, and acute lymphocytic leukemia developed 2 to 8 years later (mean 4.5 years). Three of the 7 patients also received alkylating agents. It is concluded that the development of acute leukemia, mostly acute myelocytic leukemia but also acute lymphocytic leukemia, during the course of Hodgkin's disease, is most likely related to radiation therapy. There is as yet insufficient evidence to implicate intensive chemotherapy in the causation of acute leukemia since in only three patients with Hodgkin's disease treated with chemotherapy alone has the development of acute leukemia been reported. It is possible, however, that chemotherapy potentiates the effect of radiotherapy. 2t is also possible that acute leukemia is part of the natural history of Hodgkin's disease and is occurring with greater frequency because of improved survival in Hodgkin's disease since the introduction of better radiotherapeutic and chemotherapeutic treatment regimens.", "contents": "Hodgkin's disease and acute leukemia. Report of eight cases and review of the literature. Eight cases of Hodgkin's disease and acute leukemia are reported. An additional 74 cases of acute myelocytic leukemia or one of its variants, 11 cases of acute lymphocytic leukemia, 12 cases of chronic myelocytic leukemia and 37 cases of chronic lymphocytic leukemia associated with Hodgkin's disease are reviewed from the literature. In 3 of the 82 patients with acute myelocytic leukemia and Hodgkin's disease, the two diseases occurred simultaneously. Of the remaining 79 patients, 76 had received radiation therapy for their Hodgkin's disease and acute myelocytic leukemia had developed 1.2 to 19 years later (mean 6.5 years). Thirty-four of these patients also received antineoplastic chemotherapy. Only three patients with Hodgkin's disease were treated with multiple chemotherapy alone; in these, Hodgkin's disease developed 1.2, 1.5 and 3.2 years later. In 4 of 11 patients with acute lymphocytic leukemia and Hodgkin's disease, the two disorders occurred simultaneously. The other seven patients were all treated with radiation for their Hodgkin's disease, and acute lymphocytic leukemia developed 2 to 8 years later (mean 4.5 years). Three of the 7 patients also received alkylating agents. It is concluded that the development of acute leukemia, mostly acute myelocytic leukemia but also acute lymphocytic leukemia, during the course of Hodgkin's disease, is most likely related to radiation therapy. There is as yet insufficient evidence to implicate intensive chemotherapy in the causation of acute leukemia since in only three patients with Hodgkin's disease treated with chemotherapy alone has the development of acute leukemia been reported. It is possible, however, that chemotherapy potentiates the effect of radiotherapy. 2t is also possible that acute leukemia is part of the natural history of Hodgkin's disease and is occurring with greater frequency because of improved survival in Hodgkin's disease since the introduction of better radiotherapeutic and chemotherapeutic treatment regimens."}
{"id": "PMID:1090159", "title": "Clinical experience with transfusion of granulocytes obtained by continuous flow filtration leukopheresis.", "content": "Granulocytes obtained by continuous flow filtration leukopheresis (CFFL) were transfused to 21 patients on 131 occasions. An average of 28.2 times 10-9 granulocytes were administered per transfusion. These cells were more than 90 per cent viable by dye exclusion, ingested latex particles normally and had almost normal bactericidal activity. Migration to skin windows was demonstrated on four of six attempts, but 51-Cr-labeling studies failed to show localization in infected areas on six occasions. Post-transfusion granulocyte count increments averaged 225/mul and were transient. Significant transfusion reactions occurred during 35 transfusions to 13 patients. Reactions occurred in some patients without demonstrable alloimmunization and after six infusions of HL-A identical or compatible cells. Definite clinical improvement was noted in three recipients. Stabilization of infection with patient survival occurred nine times, and progression of infection with death eight times. Granulocytes obtained by CFFL are viable and functional. Their transfusion is not without risk and must still be considered an investigative procedure of suggestive but as yet unproved clinical efficacy.", "contents": "Clinical experience with transfusion of granulocytes obtained by continuous flow filtration leukopheresis. Granulocytes obtained by continuous flow filtration leukopheresis (CFFL) were transfused to 21 patients on 131 occasions. An average of 28.2 times 10-9 granulocytes were administered per transfusion. These cells were more than 90 per cent viable by dye exclusion, ingested latex particles normally and had almost normal bactericidal activity. Migration to skin windows was demonstrated on four of six attempts, but 51-Cr-labeling studies failed to show localization in infected areas on six occasions. Post-transfusion granulocyte count increments averaged 225/mul and were transient. Significant transfusion reactions occurred during 35 transfusions to 13 patients. Reactions occurred in some patients without demonstrable alloimmunization and after six infusions of HL-A identical or compatible cells. Definite clinical improvement was noted in three recipients. Stabilization of infection with patient survival occurred nine times, and progression of infection with death eight times. Granulocytes obtained by CFFL are viable and functional. Their transfusion is not without risk and must still be considered an investigative procedure of suggestive but as yet unproved clinical efficacy."}
{"id": "PMID:1090160", "title": "Drug-induced immune hemolytic anemia.", "content": "Drug administration causes from 16 to 18 per cent of cases of acquired immune hemolytic anemia. The pathogenesis of erythrocyte sensitization by drug-related antibody with or without fixation of complement is variable, and there is a relationship between the responsible drug, the mechanism of red cell sensitization, clinical manifestations and laboratory methods of diagnosis. Drugs such as phenacetin and quinidine form a complex with the antidrug antibody, and the immune complex attaches to red cells usually fixing complement and causing acute intravascular hemolysis. Other drugs (e.g., penicillins), when given in large doses, coat normal red cells in vivo and in some patients a high titer IgG anti-drug antibody develops which reacts with the coated cells. Hemolytic anemia may develop with red cell destruction being primarily extravascular. Cephalosporins cause positive direct antiglobulin tests in a small percentage of patients either by the same mechanism as penicillins or by modification of the red cell membrane leading to nonimmunologic absorption of serum proteins. Hemolytic anemia has been reported only rarely. A few drugs (notably alpha methyldopa) cause the development of autoimmune hemolytic anemia. Knowledge of clinical manifestations and laboratory aids to diagnosis is necessary to distinguish immunohematologic abnormalities caused by drugs from other causes.", "contents": "Drug-induced immune hemolytic anemia. Drug administration causes from 16 to 18 per cent of cases of acquired immune hemolytic anemia. The pathogenesis of erythrocyte sensitization by drug-related antibody with or without fixation of complement is variable, and there is a relationship between the responsible drug, the mechanism of red cell sensitization, clinical manifestations and laboratory methods of diagnosis. Drugs such as phenacetin and quinidine form a complex with the antidrug antibody, and the immune complex attaches to red cells usually fixing complement and causing acute intravascular hemolysis. Other drugs (e.g., penicillins), when given in large doses, coat normal red cells in vivo and in some patients a high titer IgG anti-drug antibody develops which reacts with the coated cells. Hemolytic anemia may develop with red cell destruction being primarily extravascular. Cephalosporins cause positive direct antiglobulin tests in a small percentage of patients either by the same mechanism as penicillins or by modification of the red cell membrane leading to nonimmunologic absorption of serum proteins. Hemolytic anemia has been reported only rarely. A few drugs (notably alpha methyldopa) cause the development of autoimmune hemolytic anemia. Knowledge of clinical manifestations and laboratory aids to diagnosis is necessary to distinguish immunohematologic abnormalities caused by drugs from other causes."}
{"id": "PMID:1090161", "title": "Syndrome and pancreatic disease, subcutaneous fat necrosis and polyserositis. Case report and review of literature.", "content": "Immunologic evaluation of a patient with pancreatitis, subcutaneous fat necrosis, pleuritis, pericarditis and synovitis is presented. The previously recognized syndrome of pancreatic disease, subcutaneous fat necrosis and arthritis is reviewed. Based on analysis of all the cases described in the English language literature it is suggested that this syndrome be expanded to include polyserositis rather than arthritis alone. Although experimental and clinical evidence tends to implicate physiocochemical tissue injury by pancreatic lipase as the primary pathogenic mechanism in this syndrome, studies in our patient suggest the possible contribution of immune-mediated injury. Supporting data include eosinophilia, biopsy demonstration of vasculitis antedating the subcutaneous fat necrosis, immunofluorescent identification of immunoglobulin G (IgG) and C3 in the pleura, and reduced levels of total hemolytic complement in the serum, and pleural and pericardial effusions.", "contents": "Syndrome and pancreatic disease, subcutaneous fat necrosis and polyserositis. Case report and review of literature. Immunologic evaluation of a patient with pancreatitis, subcutaneous fat necrosis, pleuritis, pericarditis and synovitis is presented. The previously recognized syndrome of pancreatic disease, subcutaneous fat necrosis and arthritis is reviewed. Based on analysis of all the cases described in the English language literature it is suggested that this syndrome be expanded to include polyserositis rather than arthritis alone. Although experimental and clinical evidence tends to implicate physiocochemical tissue injury by pancreatic lipase as the primary pathogenic mechanism in this syndrome, studies in our patient suggest the possible contribution of immune-mediated injury. Supporting data include eosinophilia, biopsy demonstration of vasculitis antedating the subcutaneous fat necrosis, immunofluorescent identification of immunoglobulin G (IgG) and C3 in the pleura, and reduced levels of total hemolytic complement in the serum, and pleural and pericardial effusions."}
{"id": "PMID:1090162", "title": "Perturbations of humoral and cellular immunity in a patient with pigmented villonodular synovitis.", "content": "Several parameters of the immune system have been studied in a patient with pigmented villonodular synovitis. Numerous immunoglobulin-synthesizing cells were found by immunofluorescent technics in both synovium and synovial fluid. Phytohemagglutinin-induced in vitro blastogenesis of peripheral blood T lymphocytes was absent whereas B-lymphocyte blastogenesis was preserved. These results indicate a necessity for evaluating immune responsiveness in other patients with pigmented villonodular synovitis.", "contents": "Perturbations of humoral and cellular immunity in a patient with pigmented villonodular synovitis. Several parameters of the immune system have been studied in a patient with pigmented villonodular synovitis. Numerous immunoglobulin-synthesizing cells were found by immunofluorescent technics in both synovium and synovial fluid. Phytohemagglutinin-induced in vitro blastogenesis of peripheral blood T lymphocytes was absent whereas B-lymphocyte blastogenesis was preserved. These results indicate a necessity for evaluating immune responsiveness in other patients with pigmented villonodular synovitis."}
{"id": "PMID:1090163", "title": "Determining antibiotic levels in the blood.", "content": "Methods for the rapid determination of antibiotic blood levels require particular organisms or special conditions or long incubation. A method was developed for the rapid determination of antibiotic blood levels which requires no special equipment and is rapid. Special stains are not required. Utilizing bacteria from standard Kirby-Bauer sensitivity plates, a suspension is prepared and a pour plate made. Organisms which are sensitive to the questioned antibiotic and resistant to other antibiotics the patient is receiving are chosen. Muller-Hinton is the best general agar. Wells are cut, and antibiotic standards, antibiotic controls, and the patient's serum introduced. Within three to four hours, zone sizes can be read and the antibiotic blood level calculated. The materials and technical facility required should not preclude any laboratory from successfully performing the method.", "contents": "Determining antibiotic levels in the blood. Methods for the rapid determination of antibiotic blood levels require particular organisms or special conditions or long incubation. A method was developed for the rapid determination of antibiotic blood levels which requires no special equipment and is rapid. Special stains are not required. Utilizing bacteria from standard Kirby-Bauer sensitivity plates, a suspension is prepared and a pour plate made. Organisms which are sensitive to the questioned antibiotic and resistant to other antibiotics the patient is receiving are chosen. Muller-Hinton is the best general agar. Wells are cut, and antibiotic standards, antibiotic controls, and the patient's serum introduced. Within three to four hours, zone sizes can be read and the antibiotic blood level calculated. The materials and technical facility required should not preclude any laboratory from successfully performing the method."}
{"id": "PMID:1090164", "title": "Adaptive Behavior Scale as a screening measure for special-education placement.", "content": "The utiltiy of the Adaptive Behavior Scale in the special-education placement of 126 institutionalized mentally retarded children and adolescents was examined. An investigation of relationships among Adaptive Behavior Scale (Part 1) scores, Wide Range Achievement Test scores, and IQ scores revealed a significant relationship between Adaptive Behavior Scale scores as well as between IQ scores and achievement test scores. Adaptive Behavior Scale and IQ were the most effective scores in providing significant discrimination between the special-education training levels. Adaptive Behavior Scale subdomain percentage scores were discussed as criteria for a computer-assisted placement of mentally retarded students in a special-education program.", "contents": "Adaptive Behavior Scale as a screening measure for special-education placement. The utiltiy of the Adaptive Behavior Scale in the special-education placement of 126 institutionalized mentally retarded children and adolescents was examined. An investigation of relationships among Adaptive Behavior Scale (Part 1) scores, Wide Range Achievement Test scores, and IQ scores revealed a significant relationship between Adaptive Behavior Scale scores as well as between IQ scores and achievement test scores. Adaptive Behavior Scale and IQ were the most effective scores in providing significant discrimination between the special-education training levels. Adaptive Behavior Scale subdomain percentage scores were discussed as criteria for a computer-assisted placement of mentally retarded students in a special-education program."}
{"id": "PMID:1090165", "title": "Macrophage migration inhibitory factor in female sterility.", "content": "Cellular sensitization against spermatozoa as antigen was studied in 21 fertile and 41 infertile female subjects. In addition, 20 virgin girls and a group of 11 women presenting positive spermagglutinating (in equal 8) and immobilizing (n equal 3) antibodies were investigated. Cellular sensitization was expressed in terms of macrophage migration inhibitory activity (MIF-reaction). For this screening program washed and pooled spermatozoa (WPS) and sonicated spermatozoa solutions (sss) were used as antigens. In 52 to 72 per cent of all cases, including primary and secondary sterility of unexplained cause, a positive MIF-reaction was encountered. Women with humoral sperm antibodies always revealed a cellular sensitization. Only the group of virgin girls demonstrated in no case a positive MIF-reaction. The need for the identification of the specific fertility-diminishing antigens is stressed as the results indicate a cellular sensitization in all cases that had sperm contact.", "contents": "Macrophage migration inhibitory factor in female sterility. Cellular sensitization against spermatozoa as antigen was studied in 21 fertile and 41 infertile female subjects. In addition, 20 virgin girls and a group of 11 women presenting positive spermagglutinating (in equal 8) and immobilizing (n equal 3) antibodies were investigated. Cellular sensitization was expressed in terms of macrophage migration inhibitory activity (MIF-reaction). For this screening program washed and pooled spermatozoa (WPS) and sonicated spermatozoa solutions (sss) were used as antigens. In 52 to 72 per cent of all cases, including primary and secondary sterility of unexplained cause, a positive MIF-reaction was encountered. Women with humoral sperm antibodies always revealed a cellular sensitization. Only the group of virgin girls demonstrated in no case a positive MIF-reaction. The need for the identification of the specific fertility-diminishing antigens is stressed as the results indicate a cellular sensitization in all cases that had sperm contact."}
{"id": "PMID:1090166", "title": "In vitro biosynthesis of TSH- and LH-releasing factors by the human placenta.", "content": "Human chorionic membrane and human placental tissue contain substances with the same elution volume as hypothalamic TRF and LRF standards during separation by carboxyl-methyl-cellulose ion-exchange chromatography. Homogenates prepared from fresh human placentas delivered at term caused release of TSH (thyroid-stimulating hormone) and LH (luteinizing hormone) when bioassayed in rats. After dialysis of placental homogentes, synthesis of biologically active TSH-and LH-releasing factors can be shown to occur in vitro following the addition of appropriate precursor amino acids and cofactors.", "contents": "In vitro biosynthesis of TSH- and LH-releasing factors by the human placenta. Human chorionic membrane and human placental tissue contain substances with the same elution volume as hypothalamic TRF and LRF standards during separation by carboxyl-methyl-cellulose ion-exchange chromatography. Homogenates prepared from fresh human placentas delivered at term caused release of TSH (thyroid-stimulating hormone) and LH (luteinizing hormone) when bioassayed in rats. After dialysis of placental homogentes, synthesis of biologically active TSH-and LH-releasing factors can be shown to occur in vitro following the addition of appropriate precursor amino acids and cofactors."}
{"id": "PMID:1090167", "title": "Oral contraceptives and vitamins: a review.", "content": "The literature concerning the influence of estrogen-containing oral contraceptives on vitamins is reviewed. The appearance of an elevated plasma concentration of vitamin A is probably without clinical importance, while there seems to be a clear connection between disturbances of vitamin B6 metabolism and mental symptoms. Low levels of folic acid and vitamin B12 have also been noted. Despite this, it is very rare that hematologic abnormalities develop during hormonal contraception. A reduced concentration of vitamin C in plasma and blood corpuscles has been reported. The clinical significance of these alterations is unknown.", "contents": "Oral contraceptives and vitamins: a review. The literature concerning the influence of estrogen-containing oral contraceptives on vitamins is reviewed. The appearance of an elevated plasma concentration of vitamin A is probably without clinical importance, while there seems to be a clear connection between disturbances of vitamin B6 metabolism and mental symptoms. Low levels of folic acid and vitamin B12 have also been noted. Despite this, it is very rare that hematologic abnormalities develop during hormonal contraception. A reduced concentration of vitamin C in plasma and blood corpuscles has been reported. The clinical significance of these alterations is unknown."}
{"id": "PMID:1090168", "title": "Bacteremia on an obstetric-gynecologic service.", "content": "In a one-year evaluation of the Obstetric-Gynecologic Services of the Los Angeles County University of Southern California Medical Center, bacteremia was confirmed on 144 occasions in 139 patients. This represented an over-all incidence of 7/1,000 admissions with gram-negative bacteremia observed in 3.1/1,000 admissions. There were four deaths in this series. The most frequently recovered aerobes were Escherichia coli, enterococci, and beta hemolytic streptococci, not Group A or D, while the most commonly isolated anaerobes were peptostreptococci, peptococci, and Bacteroides. These patterns of bacterial isolation should be acknowledged in antibiotic strategies for septic patients. There is a positive correlation between the incidence of intrapartum maternal and fetal monitoring and postpartum maternal bacteremia. The oncology patients were the most seriously ill women with bacteremia.", "contents": "Bacteremia on an obstetric-gynecologic service. In a one-year evaluation of the Obstetric-Gynecologic Services of the Los Angeles County University of Southern California Medical Center, bacteremia was confirmed on 144 occasions in 139 patients. This represented an over-all incidence of 7/1,000 admissions with gram-negative bacteremia observed in 3.1/1,000 admissions. There were four deaths in this series. The most frequently recovered aerobes were Escherichia coli, enterococci, and beta hemolytic streptococci, not Group A or D, while the most commonly isolated anaerobes were peptostreptococci, peptococci, and Bacteroides. These patterns of bacterial isolation should be acknowledged in antibiotic strategies for septic patients. There is a positive correlation between the incidence of intrapartum maternal and fetal monitoring and postpartum maternal bacteremia. The oncology patients were the most seriously ill women with bacteremia."}
{"id": "PMID:1090169", "title": "Pituitary gonadotropin responses to synthetic luteinizing hormone-releasing hormone in patients with typical and atypical polycystic ovary disease.", "content": "Synthetic luteinizing hormone-releasing hormone (LH-RH) was administered intravenously to 17 women with polycystic ovary disease (PPCO), 16 women with hypothalamic amenorrhea (HA), and nine women with normal cycles. The serum levels of follicle-stimulating hormone (FHS) and luteinizing hormone (LH) were measured at frequent intervals before and after LH-RH injection. The PCO patients were arbitrarily divided into two groups on the basis of ovarian morphology. The eight patients with large, \"typical\" PCO's (Type I) showed a greater LH response and a lower FSH response to LH-RH than did the nine patients with smaller, \"atypical\" PCO's (Type II). The LH response in Type I co was also greater than that in HA, but the FSH response in both types of PCO was significantly less than that in HA. The gonadotropin responses to LH-RH in Type I PCO were quite similar to those occurring in normal women during the two to three days prededing ovulation. The results suggest that excessive LH secretion and/or impaired FSH secretion may be etiologically significant in PCO but do not clearly differentiate whether the stimulus for this pattern of gonadortopin secretion occurs at the hypothatamic or pituitary level.", "contents": "Pituitary gonadotropin responses to synthetic luteinizing hormone-releasing hormone in patients with typical and atypical polycystic ovary disease. Synthetic luteinizing hormone-releasing hormone (LH-RH) was administered intravenously to 17 women with polycystic ovary disease (PPCO), 16 women with hypothalamic amenorrhea (HA), and nine women with normal cycles. The serum levels of follicle-stimulating hormone (FHS) and luteinizing hormone (LH) were measured at frequent intervals before and after LH-RH injection. The PCO patients were arbitrarily divided into two groups on the basis of ovarian morphology. The eight patients with large, \"typical\" PCO's (Type I) showed a greater LH response and a lower FSH response to LH-RH than did the nine patients with smaller, \"atypical\" PCO's (Type II). The LH response in Type I co was also greater than that in HA, but the FSH response in both types of PCO was significantly less than that in HA. The gonadotropin responses to LH-RH in Type I PCO were quite similar to those occurring in normal women during the two to three days prededing ovulation. The results suggest that excessive LH secretion and/or impaired FSH secretion may be etiologically significant in PCO but do not clearly differentiate whether the stimulus for this pattern of gonadortopin secretion occurs at the hypothatamic or pituitary level."}
{"id": "PMID:1090171", "title": "The present status of prenatal detection of neural tube defects.", "content": "In experimentally induced myelocele in rats, efforts to find neural cells in amniotic fluid (AF) were unsuccessful. Creatine phosphokinase (CPK) and aldolase concentrations studied in serum of 118 and cerebrospinal fluid (CSF) in 9 patients with myelomeningocele showed serum CPK to be significantly elevated and more responsive to additional muscle injury than aldolase, but both enzymes appeared in lower concentrations in patients with myelomeningocele than those with infantile atrophy or cerebral palsy. In CSF, CPK, and aldolase concentrations averaged 4.2 I.U. and 2.7 S.L.U. per milliliter, respectively. Significant CPK elevation (p less than 0.001) was also found in AF from myeloschitic fetuses and maternal rat serum. Although these findings suggest that increased CPK concentration is an indicator of myelocele in rats, the technique is impractical for prenatal detection of human fetus occurs too late in gestation. This does not, however, preclude the value of CPK for detecting onset of paraparesis. In all myeloschitic human fetuses, the CSF communicates directly with AF for at least 3 to 4 weeks. This implies that CSF is probably the principal source of increased alpha-fetoprotein concentration encountered in AF of all pregnancies with NTD. When biological variables are recognized, it is evident that increased concentration of amniotic fluid alpha fetoprotein is a reliable indicator of fetuses with open myelocele and/or anenciphalus.", "contents": "The present status of prenatal detection of neural tube defects. In experimentally induced myelocele in rats, efforts to find neural cells in amniotic fluid (AF) were unsuccessful. Creatine phosphokinase (CPK) and aldolase concentrations studied in serum of 118 and cerebrospinal fluid (CSF) in 9 patients with myelomeningocele showed serum CPK to be significantly elevated and more responsive to additional muscle injury than aldolase, but both enzymes appeared in lower concentrations in patients with myelomeningocele than those with infantile atrophy or cerebral palsy. In CSF, CPK, and aldolase concentrations averaged 4.2 I.U. and 2.7 S.L.U. per milliliter, respectively. Significant CPK elevation (p less than 0.001) was also found in AF from myeloschitic fetuses and maternal rat serum. Although these findings suggest that increased CPK concentration is an indicator of myelocele in rats, the technique is impractical for prenatal detection of human fetus occurs too late in gestation. This does not, however, preclude the value of CPK for detecting onset of paraparesis. In all myeloschitic human fetuses, the CSF communicates directly with AF for at least 3 to 4 weeks. This implies that CSF is probably the principal source of increased alpha-fetoprotein concentration encountered in AF of all pregnancies with NTD. When biological variables are recognized, it is evident that increased concentration of amniotic fluid alpha fetoprotein is a reliable indicator of fetuses with open myelocele and/or anenciphalus."}
{"id": "PMID:1090172", "title": "Volume and sodium concentration studies in 300 saline-induced abortions.", "content": "In an attempt to clarify the role of volume changes and Na concentration in saline-induced midtrimester abortions, 800 amniotic fluid samples were studied in 300 cases. The immediate postinfusion values of Na ranged as high as 3,000 mEg. per liter and as low as 1,200 mEg. per liter, still resulting in abortion. In earlier gestation, higher Na concentration and greater volume increase was needed for successful induction of abortion. Volume increase, within limits, compensated for Na concentration decrease. Osmotically induced volume changes were proportionatenate to the advancing stage of gestation.", "contents": "Volume and sodium concentration studies in 300 saline-induced abortions. In an attempt to clarify the role of volume changes and Na concentration in saline-induced midtrimester abortions, 800 amniotic fluid samples were studied in 300 cases. The immediate postinfusion values of Na ranged as high as 3,000 mEg. per liter and as low as 1,200 mEg. per liter, still resulting in abortion. In earlier gestation, higher Na concentration and greater volume increase was needed for successful induction of abortion. Volume increase, within limits, compensated for Na concentration decrease. Osmotically induced volume changes were proportionatenate to the advancing stage of gestation."}
{"id": "PMID:1090173", "title": "Infections with mycoplasma and bacteria in induced midtrimester abortion and fetal loss.", "content": "Placentas and fetuses from cases of induced midtrimester abortion and fetal loss (13 weeks of pregnancy or more) were examined microbiologically. In the series of midtrimester fetal loss, bacteria were isolated in 14 per cent and genital Mycoplasma in 37 per cent of the placentas. In induced abortion, 18 per cent of the placentas grew bacteria and in only one case out of 27 was a Mycoplasma strain isolated. Fetal organs yielded cultures positive for bacteria in 16 per cent and for genital Mycoplasma in 23 per cent of the spontaneous fetal loss material, whereas pyogenic cocci were isolated from fetal organs in 18 per cent and Mycoplasma in no case of induced abortion. In the positive cases, the fetal lungs were most often infected (in 34 out of 37 positive cases of spontaneous and 4 out of 5 positive cases of induced abortion). On the other hand, microorganisms could be isolated from the brain in only 5 out of 28 cases of spontaneous and none of induced abortion. In the series of fetal loss, positive microbiologic findings from the fetal organs seemed correlated to prolonged bleeding before abortion or labor and to premature rupture of membranes.", "contents": "Infections with mycoplasma and bacteria in induced midtrimester abortion and fetal loss. Placentas and fetuses from cases of induced midtrimester abortion and fetal loss (13 weeks of pregnancy or more) were examined microbiologically. In the series of midtrimester fetal loss, bacteria were isolated in 14 per cent and genital Mycoplasma in 37 per cent of the placentas. In induced abortion, 18 per cent of the placentas grew bacteria and in only one case out of 27 was a Mycoplasma strain isolated. Fetal organs yielded cultures positive for bacteria in 16 per cent and for genital Mycoplasma in 23 per cent of the spontaneous fetal loss material, whereas pyogenic cocci were isolated from fetal organs in 18 per cent and Mycoplasma in no case of induced abortion. In the positive cases, the fetal lungs were most often infected (in 34 out of 37 positive cases of spontaneous and 4 out of 5 positive cases of induced abortion). On the other hand, microorganisms could be isolated from the brain in only 5 out of 28 cases of spontaneous and none of induced abortion. In the series of fetal loss, positive microbiologic findings from the fetal organs seemed correlated to prolonged bleeding before abortion or labor and to premature rupture of membranes."}
{"id": "PMID:1090174", "title": "Evaluation of Deladumone OB in the suppression of postpartum lactation.", "content": "Of 235 patients receiving Deladumone OB, more than 39 per cent complained of breast pain or swelling; 83 per cent were pleased with the medication. Of 215 patients receiving a placebo, more than 82 per cent complained of breast pain or swelling; 67.5 per cent were pleased with the medication.", "contents": "Evaluation of Deladumone OB in the suppression of postpartum lactation. Of 235 patients receiving Deladumone OB, more than 39 per cent complained of breast pain or swelling; 83 per cent were pleased with the medication. Of 215 patients receiving a placebo, more than 82 per cent complained of breast pain or swelling; 67.5 per cent were pleased with the medication."}
{"id": "PMID:1090175", "title": "Calcium metabolism in pregnancy: a review.", "content": "Calcium metabolism in pregnancy is a complex process involving calcium, phosphorus, vitamin D, parathyroid hormone (PTH), and calcitonin (CT). Calcium absorption is enhanced in pregnancy, and increased storage in the maternal skeleton probably occurs as well. Adequate amounts are provided by the current Recommended Dietary Allowance of 1,200 mg. daily which can be met readily by natural foods, specifically milk. If supplemental calcium is given, a nonphosphate salt is probably advisable, since some evidence suggests that excessive phosphate intake may be related to leg cramps in pregnancy. Vitamin D is necessary for optimal calcium utilization in pregnancy, although the possibility of fetal toxicity with overdosage has been suggested. From a review of available information with respect to maternal-perinatal calcium interrelationships, I propose the following hypothesis: While total maternal serum calcium declines during pregnancy because of the physiologic hypoalbuminemia, the level of ionic calcium remains constant, in part, at least, because of increasing maternal PTH output. The placenta plays a primary role in fetal calcium metabolism by transporting calcium ions from the mother to the fetus against a concentration gradient. Relatively high fetal ionic calcium levels cause suppression of PTH and stimulation of CT in the fetus, facilitating growth of the fetal skeleton. With sudden loss of the placental source of calcium at birth, the newborn infant becomes functionally hypoparathyroid and/or hypercalcitonemic, and the serum calcium level declines until 3 to 4 days of life when PTH rises and CT falls with a resultant slight rise in calcium.", "contents": "Calcium metabolism in pregnancy: a review. Calcium metabolism in pregnancy is a complex process involving calcium, phosphorus, vitamin D, parathyroid hormone (PTH), and calcitonin (CT). Calcium absorption is enhanced in pregnancy, and increased storage in the maternal skeleton probably occurs as well. Adequate amounts are provided by the current Recommended Dietary Allowance of 1,200 mg. daily which can be met readily by natural foods, specifically milk. If supplemental calcium is given, a nonphosphate salt is probably advisable, since some evidence suggests that excessive phosphate intake may be related to leg cramps in pregnancy. Vitamin D is necessary for optimal calcium utilization in pregnancy, although the possibility of fetal toxicity with overdosage has been suggested. From a review of available information with respect to maternal-perinatal calcium interrelationships, I propose the following hypothesis: While total maternal serum calcium declines during pregnancy because of the physiologic hypoalbuminemia, the level of ionic calcium remains constant, in part, at least, because of increasing maternal PTH output. The placenta plays a primary role in fetal calcium metabolism by transporting calcium ions from the mother to the fetus against a concentration gradient. Relatively high fetal ionic calcium levels cause suppression of PTH and stimulation of CT in the fetus, facilitating growth of the fetal skeleton. With sudden loss of the placental source of calcium at birth, the newborn infant becomes functionally hypoparathyroid and/or hypercalcitonemic, and the serum calcium level declines until 3 to 4 days of life when PTH rises and CT falls with a resultant slight rise in calcium."}
{"id": "PMID:1090177", "title": "Puerperal endometritis: a prospective microbiologic study.", "content": "This study was undertaken to determine the flora in the endometrial cavity of two groups of puerperal patients: 47 afebrile control subjects and 27 with endometritis. A transcervical culturing technique designed to avoid cervical contamination and to preserve aerobes, anaerobes, and mycoplasma was employed, and bacterial colony counts were reported semiquantitatively. No difference was found in the flora of these two groups. Furthermore, the most common pathogenic organisms (in decreasing order of frequency) were peptostreptococci, peptococci, Bacteroides sp., gamma-streptococci, enterococci, beta-streptococci, alpha-streptococci, and E. coli.", "contents": "Puerperal endometritis: a prospective microbiologic study. This study was undertaken to determine the flora in the endometrial cavity of two groups of puerperal patients: 47 afebrile control subjects and 27 with endometritis. A transcervical culturing technique designed to avoid cervical contamination and to preserve aerobes, anaerobes, and mycoplasma was employed, and bacterial colony counts were reported semiquantitatively. No difference was found in the flora of these two groups. Furthermore, the most common pathogenic organisms (in decreasing order of frequency) were peptostreptococci, peptococci, Bacteroides sp., gamma-streptococci, enterococci, beta-streptococci, alpha-streptococci, and E. coli."}
{"id": "PMID:1090178", "title": "Sperm-agglutinating antibodies and testicular morphology in fifty-nine men with azoospermia or cryptozoospermia.", "content": "The relationship between the state of the germinal epithelium and the type and titer of circulating sperm-agglutinating antibodies has been investigated in a series of 59 azoospermic or occasionally cryptozoospermic men. The patients were grouped according to the condition of the germinal epithelium as observed from testicular biopsy specimens, as well as to type and titer of circulating sperm-agglutinating antibodies investigated by a previously described microagglutination technique. Evidence is presented to suggest that the presence of mature spermatozoa in the testicular structures may be a prerequisite for the spontaneous production of circulating sperm-agglutinating antibodies, at least of the head-to-tail (H-T) agglutinating type. Furthermore, these circulating H-T sperm-agglutinating antibodies, once they are formed, do not seem to interfere adversely with the germinal epithelium of the carrier.", "contents": "Sperm-agglutinating antibodies and testicular morphology in fifty-nine men with azoospermia or cryptozoospermia. The relationship between the state of the germinal epithelium and the type and titer of circulating sperm-agglutinating antibodies has been investigated in a series of 59 azoospermic or occasionally cryptozoospermic men. The patients were grouped according to the condition of the germinal epithelium as observed from testicular biopsy specimens, as well as to type and titer of circulating sperm-agglutinating antibodies investigated by a previously described microagglutination technique. Evidence is presented to suggest that the presence of mature spermatozoa in the testicular structures may be a prerequisite for the spontaneous production of circulating sperm-agglutinating antibodies, at least of the head-to-tail (H-T) agglutinating type. Furthermore, these circulating H-T sperm-agglutinating antibodies, once they are formed, do not seem to interfere adversely with the germinal epithelium of the carrier."}
{"id": "PMID:1090179", "title": "The endothelium of failed corneal grafts.", "content": "Corneal buttons of failed grafts were obtained at the time of regrafting and studied with the scanning electron microscope. The condititions studied varied from failed donor tissue to graft rejections and herpetic endothelial disease. The scanning electron microscope offers a tridimensional, panoramic high-power view of pathologic endothelium that can be correlated to the clinical picture and light or transmission electron microscopy. The endothelium of recent grafts may be damaged by organized blood clots; leukocytes were present over the graft endothelium, possibly as a normal reaction to surgery. Herpetic infection of the graft may cause death of endothelial cells and formation of granulomatous lesions, with damage to Descemet's membrane. A large detachment and rupture of Descemet's membrane was found in a case of wound dehiscense.", "contents": "The endothelium of failed corneal grafts. Corneal buttons of failed grafts were obtained at the time of regrafting and studied with the scanning electron microscope. The condititions studied varied from failed donor tissue to graft rejections and herpetic endothelial disease. The scanning electron microscope offers a tridimensional, panoramic high-power view of pathologic endothelium that can be correlated to the clinical picture and light or transmission electron microscopy. The endothelium of recent grafts may be damaged by organized blood clots; leukocytes were present over the graft endothelium, possibly as a normal reaction to surgery. Herpetic infection of the graft may cause death of endothelial cells and formation of granulomatous lesions, with damage to Descemet's membrane. A large detachment and rupture of Descemet's membrane was found in a case of wound dehiscense."}
{"id": "PMID:1090180", "title": "Immunologic  aspects of cicatricial pemphigoid.", "content": "A 56-year-old woman presented with the typical findings of cicatricial pemphigoid: vesicular lesions of the mucous membranes and skin. Ocular involvement included symblepharon and dryness. A regimen of 80 mg of oral trimcinolone per day healed the lesions of the skin and mucous membrane. The patient received three injections of 0.5 ml of triamcinolone acetonide into the area of the symblepharon without apparent effect on the ocular disease. Direct immunofluorescent microscopy of the patient's conjunctiva revealed fluorescence of the basement membrane when incubated with fluorescein labeled anti-IgG. The finding of immunologlobulin fixed to be basement membrane in cicatricial pepmhigoid indicates some type of immune mechanism, and would seem to place this entity in the spectrum of the pemphigus-pemphigoid group.", "contents": "Immunologic  aspects of cicatricial pemphigoid. A 56-year-old woman presented with the typical findings of cicatricial pemphigoid: vesicular lesions of the mucous membranes and skin. Ocular involvement included symblepharon and dryness. A regimen of 80 mg of oral trimcinolone per day healed the lesions of the skin and mucous membrane. The patient received three injections of 0.5 ml of triamcinolone acetonide into the area of the symblepharon without apparent effect on the ocular disease. Direct immunofluorescent microscopy of the patient's conjunctiva revealed fluorescence of the basement membrane when incubated with fluorescein labeled anti-IgG. The finding of immunologlobulin fixed to be basement membrane in cicatricial pepmhigoid indicates some type of immune mechanism, and would seem to place this entity in the spectrum of the pemphigus-pemphigoid group."}
{"id": "PMID:1090183", "title": "Capillary density in developing and healing tuberculous lesions produced by BCG in rabbits. A quantitative study.", "content": "Dermal tuberculous lesions were produced in rabbits by the intradermal injection of BCG. At various times after infection, anesthetized animals were perfused with a gelatin-colloidal carbon medium via the abdominal aorta. The capillary density of the nonnecrotic granulation tissue in the lesions was determined quantitatively by counting the capillaries under an ocular grid of a microscope. The capillary density in normal skin near the lesions was 3.8 plus or minus 0.5 in millimetersof capillary lengths per square millimeter in 250-mu tissue sections. The capillary density of the nonnecrotic tissue in BCGlesions averaged 6.1 plus or minus 0.6 mm/sq mm, an increase of 60%. The capillary density remained more or less constant as the BCG lesions grew and then regressed. The development of delayed hypersensitivity seemed to increase the capillary density, but this increase may have been a response to an extension of the necrosis at the time delayed hypersensitivity developed. Capillary densities in tuberculin reactions resembled those in BCG lesions. In the early stages, the increaseed capillary network of dermal BCG lesions was derived mainly from the subpapillary vascular plexus of the deep dermis supplied branches that surrounded the lower half of the caseous necrotic center and anastomosed with capillaries from the subpapillary plexus supplying the upper  half. When the necrotic center extended, nearby capillaries thrombosed and in turn became necrotic. Peripherally, new capillaries formed and anastomosed with existing capillaries. From these vessels, mononuclear phagocytes emigrated, destroyed the tubercle bacilli, and enabled the lesion to heal. In the BCG lesions at all stages of development and healing, the capillary network in the nonnecrotic areas seemed adequate to supply and nourish the defense cells controlling the infection.", "contents": "Capillary density in developing and healing tuberculous lesions produced by BCG in rabbits. A quantitative study. Dermal tuberculous lesions were produced in rabbits by the intradermal injection of BCG. At various times after infection, anesthetized animals were perfused with a gelatin-colloidal carbon medium via the abdominal aorta. The capillary density of the nonnecrotic granulation tissue in the lesions was determined quantitatively by counting the capillaries under an ocular grid of a microscope. The capillary density in normal skin near the lesions was 3.8 plus or minus 0.5 in millimetersof capillary lengths per square millimeter in 250-mu tissue sections. The capillary density of the nonnecrotic tissue in BCGlesions averaged 6.1 plus or minus 0.6 mm/sq mm, an increase of 60%. The capillary density remained more or less constant as the BCG lesions grew and then regressed. The development of delayed hypersensitivity seemed to increase the capillary density, but this increase may have been a response to an extension of the necrosis at the time delayed hypersensitivity developed. Capillary densities in tuberculin reactions resembled those in BCG lesions. In the early stages, the increaseed capillary network of dermal BCG lesions was derived mainly from the subpapillary vascular plexus of the deep dermis supplied branches that surrounded the lower half of the caseous necrotic center and anastomosed with capillaries from the subpapillary plexus supplying the upper  half. When the necrotic center extended, nearby capillaries thrombosed and in turn became necrotic. Peripherally, new capillaries formed and anastomosed with existing capillaries. From these vessels, mononuclear phagocytes emigrated, destroyed the tubercle bacilli, and enabled the lesion to heal. In the BCG lesions at all stages of development and healing, the capillary network in the nonnecrotic areas seemed adequate to supply and nourish the defense cells controlling the infection."}
{"id": "PMID:1090184", "title": "An analysis of the Phagocytic potential of multinucleate foreign body giant cells.", "content": "Multinucleate giant cells were collected by subcutaneous implantation of plastic films into mice. The attached cells were challanged in vitro with staphylococci, yeasts and sheep erythrocytes treated with either glutaraldehyde or isologous or heterologous antiserum. Cells containing more than seven nuclei rarely phagocytized yeasts or staphylococci, and the uptake and ingestion of sheep erythrocytes treated with heterologous antiserum was equally infrequent. Many sheep erythrocytes treated with isologous antiserum or glutaraldehyde attached to giant cells. When the adherent erythrocytes were related to the increased size of the multinucleate cell by dividing the number attaching by the number of nuclei in the giant cell, a progressive relative reduction was demonstrated as the nuclear content increased. It is suggested that these phenomena are due to the loss of surface receptors subsequent to fusion during the formation of multinucleate cells.", "contents": "An analysis of the Phagocytic potential of multinucleate foreign body giant cells. Multinucleate giant cells were collected by subcutaneous implantation of plastic films into mice. The attached cells were challanged in vitro with staphylococci, yeasts and sheep erythrocytes treated with either glutaraldehyde or isologous or heterologous antiserum. Cells containing more than seven nuclei rarely phagocytized yeasts or staphylococci, and the uptake and ingestion of sheep erythrocytes treated with heterologous antiserum was equally infrequent. Many sheep erythrocytes treated with isologous antiserum or glutaraldehyde attached to giant cells. When the adherent erythrocytes were related to the increased size of the multinucleate cell by dividing the number attaching by the number of nuclei in the giant cell, a progressive relative reduction was demonstrated as the nuclear content increased. It is suggested that these phenomena are due to the loss of surface receptors subsequent to fusion during the formation of multinucleate cells."}
{"id": "PMID:1090186", "title": "Natural selection on the dentition of an Arikara population.", "content": "The tooth crowns of most mammals do not change in size except by attrition. Therefore, by comparing age groups within a population and finding differences in size or variation, the effects of such agencies as directional or stabilizing selection can be demonstrated. Mesiodistal and buccolingual measurements were taken on the maxillary and mandibular permanent dentition of a protohistoric Arikara Indian population from South Dakota. The tooth sizes of juveniles ages 6 through 15 were compared to those of adults ages 16 through 20. The two age groups were also compared by the cross-sectional areas of the posterior teeth. When a difference between age groups was demonstrated in either size or variation, a selection intensity was estimated using published graphs. Results from the comparisons show that adults, in general, have larger and less variable teeth than juveniles. The data suggest the possibility that both directional and stabilizing selection were operating on the Arikara dentition. Furthermore, the selection intensities suggest that selection was operating not on the individual teeth but on functional complexes such as the posterior teeth. It appears that in populations like the Arikara where crown attrition is severe, selection will be in the direction of large teeth.", "contents": "Natural selection on the dentition of an Arikara population. The tooth crowns of most mammals do not change in size except by attrition. Therefore, by comparing age groups within a population and finding differences in size or variation, the effects of such agencies as directional or stabilizing selection can be demonstrated. Mesiodistal and buccolingual measurements were taken on the maxillary and mandibular permanent dentition of a protohistoric Arikara Indian population from South Dakota. The tooth sizes of juveniles ages 6 through 15 were compared to those of adults ages 16 through 20. The two age groups were also compared by the cross-sectional areas of the posterior teeth. When a difference between age groups was demonstrated in either size or variation, a selection intensity was estimated using published graphs. Results from the comparisons show that adults, in general, have larger and less variable teeth than juveniles. The data suggest the possibility that both directional and stabilizing selection were operating on the Arikara dentition. Furthermore, the selection intensities suggest that selection was operating not on the individual teeth but on functional complexes such as the posterior teeth. It appears that in populations like the Arikara where crown attrition is severe, selection will be in the direction of large teeth."}
{"id": "PMID:1090187", "title": "Otosclerotic stapedial footplate fixation in an 18th century burial.", "content": "Fixation of the footplate of the stapes by otosclerosis had been found in the left ear of a probable Spaniard who died about 200 years ago and was buried in the Tuscon Presidio. Previous studies on North and South Dakota American Indian crania of about the same time period did not demonstrate any stapedial fixation in 2,600 burials (4,064 temporal bones), 1,416 of which were over 16 years of age at death. The Tucson burial would indicate that this abnormality can be demonstrated in old interred skeletal material. Our other studies suggest that this disease process was not common in the prehistoric and historic Indian populations of the Dakotas.", "contents": "Otosclerotic stapedial footplate fixation in an 18th century burial. Fixation of the footplate of the stapes by otosclerosis had been found in the left ear of a probable Spaniard who died about 200 years ago and was buried in the Tuscon Presidio. Previous studies on North and South Dakota American Indian crania of about the same time period did not demonstrate any stapedial fixation in 2,600 burials (4,064 temporal bones), 1,416 of which were over 16 years of age at death. The Tucson burial would indicate that this abnormality can be demonstrated in old interred skeletal material. Our other studies suggest that this disease process was not common in the prehistoric and historic Indian populations of the Dakotas."}
{"id": "PMID:1090188", "title": "The language capability of Neanderthal man.", "content": "Considerable publicity has been given a recent article by Lieberman and Crelin ('71) which argues that Neanderthal man lacked the physical features necessary for speech. This present paper presents statistics on some modern men with normal speech who show physical features in common with Neanderthal man. The brain of Neanderthal man was as large as, or larger than, that of modern man. The brain reflected by endocranial cast of the La Chapelle-aux-Saints skull resembles that of modern man in an area important for speech and therapy suggests Neanderthal man had the neural development necessary for language.", "contents": "The language capability of Neanderthal man. Considerable publicity has been given a recent article by Lieberman and Crelin ('71) which argues that Neanderthal man lacked the physical features necessary for speech. This present paper presents statistics on some modern men with normal speech who show physical features in common with Neanderthal man. The brain of Neanderthal man was as large as, or larger than, that of modern man. The brain reflected by endocranial cast of the La Chapelle-aux-Saints skull resembles that of modern man in an area important for speech and therapy suggests Neanderthal man had the neural development necessary for language."}
{"id": "PMID:1090189", "title": "Cribra orbitalia in the aborigines of Hawaii and Australia.", "content": "Cranii of 53 Hawaiian aboriginal infants and children, and 45 from Austrailian aboriginal children were inspected. Cribra orbitalia was present in 22.8% of the former and 26.6% of the latter; osteoporotic pitting (symmetrical osteoporosis; porotic hyperostosis) was also present in the latter. The frequency compares favorably with that found in pre-Columbian North American Pueblo Indians, 24.7%. It is associated with a widespread skeletal involvement suggestive of an active bone marrow. The findings support the concept that cribra orbitalia is related to symmetrical osteoporosis and that it may be associated with a blood disorder.", "contents": "Cribra orbitalia in the aborigines of Hawaii and Australia. Cranii of 53 Hawaiian aboriginal infants and children, and 45 from Austrailian aboriginal children were inspected. Cribra orbitalia was present in 22.8% of the former and 26.6% of the latter; osteoporotic pitting (symmetrical osteoporosis; porotic hyperostosis) was also present in the latter. The frequency compares favorably with that found in pre-Columbian North American Pueblo Indians, 24.7%. It is associated with a widespread skeletal involvement suggestive of an active bone marrow. The findings support the concept that cribra orbitalia is related to symmetrical osteoporosis and that it may be associated with a blood disorder."}
{"id": "PMID:1090190", "title": "Metrical analysis of Fontechevade II.", "content": "The Font\u00e9chevade pi calotte is analyzed by principal coordinates on the basis of 12 measurements. It most closely resembles Classic Neandertals and the Steinheim cranium in shape, showing no special similarity to modern man.", "contents": "Metrical analysis of Fontechevade II. The Font\u00e9chevade pi calotte is analyzed by principal coordinates on the basis of 12 measurements. It most closely resembles Classic Neandertals and the Steinheim cranium in shape, showing no special similarity to modern man."}
{"id": "PMID:1090191", "title": "Kinetics of unidirectional leucine transport into brain: effects of isoleucine, valine, and anoxia.", "content": "The rate of unidirectional uptake, v, of L-[4,5-3H]leucine was studied in 17 isolated dog brains by means of an indicator-dilution technique using 22Na as the intravascular reference. The arterial leucine concentration was varied in increments by adding unlabeled leucine to the blood and v was determined after each change. The valine and isoleucine concentrations were varied independently to permit evaluation of their effect on leucine transport. A preliminary analysis indicated that both valine and isoleucine are competitive inhibitors. Therefore, all data were fitted to an equation that describes Michaelis-Menten kinetics in the presence of two competitive inhibitors. These calculations yielded an apparent Km for leucine transport of 1.58 mM plus or minus .28 SE, a V max of 0.323 mumol/g per min plus or minus .035 SE, and an apparent K i for inhibition of leucine transport of 1.76 mM plus or minus .34 SE for valine and 0.73 mM plus or minus .14 SE for isoleucine. In four isolated brains perfused with blood having a constant leucine level, indicator-dilution injections were made before, and at 1, 5, and 10 min after the start of perfusion with anoxic blood. These findings showed that, unlike glucose transport (Brain Res. 67: 307-316, 1974), the rate of lucine transport is unaffected by 10 min of anoxia.", "contents": "Kinetics of unidirectional leucine transport into brain: effects of isoleucine, valine, and anoxia. The rate of unidirectional uptake, v, of L-[4,5-3H]leucine was studied in 17 isolated dog brains by means of an indicator-dilution technique using 22Na as the intravascular reference. The arterial leucine concentration was varied in increments by adding unlabeled leucine to the blood and v was determined after each change. The valine and isoleucine concentrations were varied independently to permit evaluation of their effect on leucine transport. A preliminary analysis indicated that both valine and isoleucine are competitive inhibitors. Therefore, all data were fitted to an equation that describes Michaelis-Menten kinetics in the presence of two competitive inhibitors. These calculations yielded an apparent Km for leucine transport of 1.58 mM plus or minus .28 SE, a V max of 0.323 mumol/g per min plus or minus .035 SE, and an apparent K i for inhibition of leucine transport of 1.76 mM plus or minus .34 SE for valine and 0.73 mM plus or minus .14 SE for isoleucine. In four isolated brains perfused with blood having a constant leucine level, indicator-dilution injections were made before, and at 1, 5, and 10 min after the start of perfusion with anoxic blood. These findings showed that, unlike glucose transport (Brain Res. 67: 307-316, 1974), the rate of lucine transport is unaffected by 10 min of anoxia."}
{"id": "PMID:1090192", "title": "Lipoate effect on carbohydrate and lipid metabolism and gastric H+ secretion.", "content": "Acid secretion (QH+) and oxygen consumption (Qo2) by frog gastric mucosae in vitro were sharply stimulated by lipoate. A rapid decline followed stimulation, subsequently falling below control values. Addition of only glucose or lactate had no effect on Qo2 or QH+. Pyruvate caused slight significant stimulation of Qo2. Any one of these compounds added to lipoate-treated mucosae increased the stimulatory effect of lipoate and markedly slowed the rate of decline subsequent to maximum stimulation. Various fatty acids had a moderate-to-high stimulatory effect on Qo2 and QH+. Lipoate added prior to the addition of fatty acids decreased the stimulatory effect of buryrate (minus 56%), decanoate (minus 87%), and palmitate (minus 60%). Propionate became an inhibitor in the presence of lipoate. Lipoate increased (plus 100%) the amount of glycogen oxidized and decreased (minus 69%) the amount of triglycerides oxidized. Lipoate-treated mucosae did not respond to histamine. Addition of glucose restored responsiveness. The results indicate that beta-oxidation of fatty acids plays a major role in the acid secretory process and is centrally involved in cyclic AMP and histamine stimulation of QH+.", "contents": "Lipoate effect on carbohydrate and lipid metabolism and gastric H+ secretion. Acid secretion (QH+) and oxygen consumption (Qo2) by frog gastric mucosae in vitro were sharply stimulated by lipoate. A rapid decline followed stimulation, subsequently falling below control values. Addition of only glucose or lactate had no effect on Qo2 or QH+. Pyruvate caused slight significant stimulation of Qo2. Any one of these compounds added to lipoate-treated mucosae increased the stimulatory effect of lipoate and markedly slowed the rate of decline subsequent to maximum stimulation. Various fatty acids had a moderate-to-high stimulatory effect on Qo2 and QH+. Lipoate added prior to the addition of fatty acids decreased the stimulatory effect of buryrate (minus 56%), decanoate (minus 87%), and palmitate (minus 60%). Propionate became an inhibitor in the presence of lipoate. Lipoate increased (plus 100%) the amount of glycogen oxidized and decreased (minus 69%) the amount of triglycerides oxidized. Lipoate-treated mucosae did not respond to histamine. Addition of glucose restored responsiveness. The results indicate that beta-oxidation of fatty acids plays a major role in the acid secretory process and is centrally involved in cyclic AMP and histamine stimulation of QH+."}
{"id": "PMID:1090193", "title": "One-year follow-up of hyperactive boys treated with imipramine or methylphenidate.", "content": "The authors report on a one-year follow-up of 76 hyperactive boys who had participated in a comparative study of methylphenidate, imipramine, and placebo. They found that there was a higher rate of discontinuance of imipramine than of methylphenidate in this sample and that both medications decreased the boys' rate of weight gain but had no significant effect on growth in height. This findings is of concern because larger doses than were used in this study are in use elsewhere. Teachers reported that the group of boys who had discontinued either medications showed continued hyperactivity and behavior problems in the classroom at one year. The two treatment groups showed continued improvement at one year in classroom and home behavior and did not suffer significantly from each other.", "contents": "One-year follow-up of hyperactive boys treated with imipramine or methylphenidate. The authors report on a one-year follow-up of 76 hyperactive boys who had participated in a comparative study of methylphenidate, imipramine, and placebo. They found that there was a higher rate of discontinuance of imipramine than of methylphenidate in this sample and that both medications decreased the boys' rate of weight gain but had no significant effect on growth in height. This findings is of concern because larger doses than were used in this study are in use elsewhere. Teachers reported that the group of boys who had discontinued either medications showed continued hyperactivity and behavior problems in the classroom at one year. The two treatment groups showed continued improvement at one year in classroom and home behavior and did not suffer significantly from each other."}
{"id": "PMID:1090199", "title": "Cervico-mediastinal haematoma following sneezing.", "content": "A case of spontaneous cervico-mediastinal haematoma is described. This is the ninth case recorded and is unusual in that the haematoma seems to have resulted from rupture of a para-oesophageal vein. The differential diagnosis and methods of investigation are discussed.", "contents": "Cervico-mediastinal haematoma following sneezing. A case of spontaneous cervico-mediastinal haematoma is described. This is the ninth case recorded and is unusual in that the haematoma seems to have resulted from rupture of a para-oesophageal vein. The differential diagnosis and methods of investigation are discussed."}
{"id": "PMID:1090200", "title": "Post-operative convulsions due to penicillin applied to the spinal theca.", "content": "Two cases are described and discussed in which penicillin powder applied to the theca at the end of laminectomy caused delayed convulsions.", "contents": "Post-operative convulsions due to penicillin applied to the spinal theca. Two cases are described and discussed in which penicillin powder applied to the theca at the end of laminectomy caused delayed convulsions."}
{"id": "PMID:1090201", "title": "Positive end-expiratory pressure during mechanical ventilation of the lungs.  A new vlave.", "content": "A valve is described which will provide positive end-expiratory pressure during mechanical ventilation of the lungs. The authors will be pleased to supply further details of specification and production on request.", "contents": "Positive end-expiratory pressure during mechanical ventilation of the lungs.  A new vlave. A valve is described which will provide positive end-expiratory pressure during mechanical ventilation of the lungs. The authors will be pleased to supply further details of specification and production on request."}
{"id": "PMID:1090202", "title": "Chloroform or ether-a literary question.", "content": "Thackeray's accurate knowledge of the pharmacology of chloroform and ether is considered and his source discussed.", "contents": "Chloroform or ether-a literary question. Thackeray's accurate knowledge of the pharmacology of chloroform and ether is considered and his source discussed."}
{"id": "PMID:1090203", "title": "Structural modifications of lutein cell gap junctions during pregnancy in the rat and the mouse.", "content": "By use of lanthanum tracer and freeze-fracture procedures it was found that granulosa-lutein cells of the pregnant mouse and rat ovaries are connected by gap junctions and septate-like zones of contact. Lutein cell gap junctions enlarge and become partially internalized by the end of the first week of gestation. Expansion of the gap junction domain appears to be due initially to intercalation of particles along borders of small gap junctions devoid of smaller non-junctional particles. The number of gap junction lined processes appearing at the cell border increases concomitantly with hypertrophy of the lutein cell during the second week of pregnancy. Strands of particulate or grooved membrane emanate from the margin of larger gap junctions undergoing interiorization. Most large gap junctions are intimately associated with elements of the smooth endoplasmic reticulum. Spherical gap junctional profiles assume a deeper location in the lutein cell and may form concentric arrays by term while true surface gap junctions appear to fragment in the post-partum corpus luteum. The modifications observed are interpreted with respect to biogenesis of the gap junction and the hormonal control of lutein cell function.", "contents": "Structural modifications of lutein cell gap junctions during pregnancy in the rat and the mouse. By use of lanthanum tracer and freeze-fracture procedures it was found that granulosa-lutein cells of the pregnant mouse and rat ovaries are connected by gap junctions and septate-like zones of contact. Lutein cell gap junctions enlarge and become partially internalized by the end of the first week of gestation. Expansion of the gap junction domain appears to be due initially to intercalation of particles along borders of small gap junctions devoid of smaller non-junctional particles. The number of gap junction lined processes appearing at the cell border increases concomitantly with hypertrophy of the lutein cell during the second week of pregnancy. Strands of particulate or grooved membrane emanate from the margin of larger gap junctions undergoing interiorization. Most large gap junctions are intimately associated with elements of the smooth endoplasmic reticulum. Spherical gap junctional profiles assume a deeper location in the lutein cell and may form concentric arrays by term while true surface gap junctions appear to fragment in the post-partum corpus luteum. The modifications observed are interpreted with respect to biogenesis of the gap junction and the hormonal control of lutein cell function."}
{"id": "PMID:1090204", "title": "SEM preparation and observations of the hen's oviduct.", "content": "The surface patterns of the oviduct of Gallus domesticus were examined by scanning electron microscopy (SEM) using several preparatory techniques. Ciliated epithelial cells predominate throughout the oviduct with non-ciliated cells approaching an equal proportion in the magnum and isthmus. Short, randomly oriented mucosal folds which characterize the infundibulum increase in height and longitudinal orientation as they approach the magnum. Here and in the isthmus they broaden and become dimpled with glandular apertures. The folds narrow again in the uterus and vagina. Cryofracture followed by SEM demonstrate the deeper mucosal folds and submucosal tubular system.", "contents": "SEM preparation and observations of the hen's oviduct. The surface patterns of the oviduct of Gallus domesticus were examined by scanning electron microscopy (SEM) using several preparatory techniques. Ciliated epithelial cells predominate throughout the oviduct with non-ciliated cells approaching an equal proportion in the magnum and isthmus. Short, randomly oriented mucosal folds which characterize the infundibulum increase in height and longitudinal orientation as they approach the magnum. Here and in the isthmus they broaden and become dimpled with glandular apertures. The folds narrow again in the uterus and vagina. Cryofracture followed by SEM demonstrate the deeper mucosal folds and submucosal tubular system."}
{"id": "PMID:1090210", "title": "Lung volumes and closing capacity with continuous positive airway pressure.", "content": "Total lung capacity, vital capacity, residual volume, and functional residual capacity were determined by body plethysmography and the single-breath oxygen (SBO2) test was performed at 0, 5, and 11 cm H20 continuous positive airway pressure in healthy, awake, seated, spontaneously breathing subjects. Mean values for the absolute lung volume at which phase IV of the SBO2 test begins (closing capacity) did not change significantly with continous positive airway pressure at 5 or 11 cm H2O. Mean total lung capacity, functional residual capacity, and residual volume increased significantly, and the mean closing volume, the lung volume above residual volume at which phase IV begins, decreased significantly with 11 cm H20 continuous positive airway pressure; differences at 5 cm H20 were not significant. The slope of the alveolar nitrogen plateau (phase III) obtained during the SBO-2 test did not change with continuous positive airway pressure.", "contents": "Lung volumes and closing capacity with continuous positive airway pressure. Total lung capacity, vital capacity, residual volume, and functional residual capacity were determined by body plethysmography and the single-breath oxygen (SBO2) test was performed at 0, 5, and 11 cm H20 continuous positive airway pressure in healthy, awake, seated, spontaneously breathing subjects. Mean values for the absolute lung volume at which phase IV of the SBO2 test begins (closing capacity) did not change significantly with continous positive airway pressure at 5 or 11 cm H2O. Mean total lung capacity, functional residual capacity, and residual volume increased significantly, and the mean closing volume, the lung volume above residual volume at which phase IV begins, decreased significantly with 11 cm H20 continuous positive airway pressure; differences at 5 cm H20 were not significant. The slope of the alveolar nitrogen plateau (phase III) obtained during the SBO-2 test did not change with continuous positive airway pressure."}
{"id": "PMID:1090205", "title": "Blood components in the treatment of acute blood loss: use of freeze-preserved red cells, platelets, and plasma proteins.", "content": "To avoid untoward reactions from blood transfusions and to make best use of the limited supply of blood, anesthesiologists and surgeons have many newly developed means at their disposal. Blood components should be separated from whole blood at the time of collection and prepared for either liquid or freeze-preservation. Citrate-phosphate-dextrose (CPD) blood should be separated into its components at room temperature within 4 hours of collection for greatest service from each collected unit. Red cell concentrates with hematocrits of 70 volumes percent can be prepared from the whole blood at the time of collection and frozen either shortly thereafter or after storage at 4 degrees C. for up to 3 weeks. Red-cell levels of 2, 3-diphosphoglycerate (2, 3-DPG) and adenosine triphosphate (ATP) can be increased by a rejuvenation process prior to freeze-preservation with either 40 percent W/V glycerol and storage at minus 80 degrees C. or with 20 percent W/V glycerol and storage at minus 150 degrees C. While hemorrhagic shock can best be managed with fresh whole blood, such blood is often not available; liquid- and freeze-preserved products serve as best substitutes. When previously-frozen washed red cells are used, crystalloid, colloid, coagulation factors, and platelets may also be required. Platelet concentrates stored at 4 degrees C. provide platelets that are hemostatically effective immediately upon infusion but have poor circulation. Platelet concentrates stored at 22 degrees C. provide platelets that have good circulation but upon transfusion have impaired hemostatic effectiveness. The coagulation factors and oncotic properties of plasma protein necessary for proper treatment of patients in hemorrhagic shock can be met by an adequate supply of fresh-frozen plasma and albumin. When liquid-stored red-cell concentrates or whole blood is given, filters must be used to remove the accumulated amorphous material, although the actual effects of the infused microaggregates are not yet known.", "contents": "Blood components in the treatment of acute blood loss: use of freeze-preserved red cells, platelets, and plasma proteins. To avoid untoward reactions from blood transfusions and to make best use of the limited supply of blood, anesthesiologists and surgeons have many newly developed means at their disposal. Blood components should be separated from whole blood at the time of collection and prepared for either liquid or freeze-preservation. Citrate-phosphate-dextrose (CPD) blood should be separated into its components at room temperature within 4 hours of collection for greatest service from each collected unit. Red cell concentrates with hematocrits of 70 volumes percent can be prepared from the whole blood at the time of collection and frozen either shortly thereafter or after storage at 4 degrees C. for up to 3 weeks. Red-cell levels of 2, 3-diphosphoglycerate (2, 3-DPG) and adenosine triphosphate (ATP) can be increased by a rejuvenation process prior to freeze-preservation with either 40 percent W/V glycerol and storage at minus 80 degrees C. or with 20 percent W/V glycerol and storage at minus 150 degrees C. While hemorrhagic shock can best be managed with fresh whole blood, such blood is often not available; liquid- and freeze-preserved products serve as best substitutes. When previously-frozen washed red cells are used, crystalloid, colloid, coagulation factors, and platelets may also be required. Platelet concentrates stored at 4 degrees C. provide platelets that are hemostatically effective immediately upon infusion but have poor circulation. Platelet concentrates stored at 22 degrees C. provide platelets that have good circulation but upon transfusion have impaired hemostatic effectiveness. The coagulation factors and oncotic properties of plasma protein necessary for proper treatment of patients in hemorrhagic shock can be met by an adequate supply of fresh-frozen plasma and albumin. When liquid-stored red-cell concentrates or whole blood is given, filters must be used to remove the accumulated amorphous material, although the actual effects of the infused microaggregates are not yet known."}
{"id": "PMID:1090207", "title": "\"Hyper-\" end-expiratory pressure in the treatment of adult respiratory insufficiency: a case report.", "content": "Postiive end-expiratory pressure (PEEP), in combination with mechanical ventilation, is efficacious in the treatment of some forms of adult respiratory insufficiency. PEEP in excess of 10 to 15 torr usually is not recommended; however, higher levels of PEEP may be required to reverse the pulmonary venous admixture. The efficacy of optimizing cardiopulmonary function with varying levels of PEEP is described.", "contents": "\"Hyper-\" end-expiratory pressure in the treatment of adult respiratory insufficiency: a case report. Postiive end-expiratory pressure (PEEP), in combination with mechanical ventilation, is efficacious in the treatment of some forms of adult respiratory insufficiency. PEEP in excess of 10 to 15 torr usually is not recommended; however, higher levels of PEEP may be required to reverse the pulmonary venous admixture. The efficacy of optimizing cardiopulmonary function with varying levels of PEEP is described."}
{"id": "PMID:1090209", "title": "Fibrinogen and albumin deficiencies associated with packed red blood cell transfusions.", "content": "Fibrinogen content was determined for each of 50 units of citrate-dextrose-phosphate (CPD)-preserved whole blood, packed red blood cells reconstituted with 250 ml. of saline, and packed red cells reconstituted with 250 ml. of purified plasma protein fraction (PPF). The total protein and albumin were measured, by electrophoresis, on each of 10 units of the three varieties of blood. The fibrinogen content of the two types of reconstituted cells was significantly lower than that of whole blood. Although the total protein/albumin content of whole blood and PPF-reconstituted red cells was similar, saline-reconstituted cells were markedly deficient in both total protein and albumin. Low fibrinogen and platelet levels subsequent to transfusion with reconstituted packed red cells can lead to an erroneous diagnosis of disseminated intravascular coagulation. Administration of large quantities of saline-reconstituted packed cells could be an etiologic factor in postoperative interstitial pulmonary edema.", "contents": "Fibrinogen and albumin deficiencies associated with packed red blood cell transfusions. Fibrinogen content was determined for each of 50 units of citrate-dextrose-phosphate (CPD)-preserved whole blood, packed red blood cells reconstituted with 250 ml. of saline, and packed red cells reconstituted with 250 ml. of purified plasma protein fraction (PPF). The total protein and albumin were measured, by electrophoresis, on each of 10 units of the three varieties of blood. The fibrinogen content of the two types of reconstituted cells was significantly lower than that of whole blood. Although the total protein/albumin content of whole blood and PPF-reconstituted red cells was similar, saline-reconstituted cells were markedly deficient in both total protein and albumin. Low fibrinogen and platelet levels subsequent to transfusion with reconstituted packed red cells can lead to an erroneous diagnosis of disseminated intravascular coagulation. Administration of large quantities of saline-reconstituted packed cells could be an etiologic factor in postoperative interstitial pulmonary edema."}
{"id": "PMID:1090220", "title": "Effects of prednisone on gastric secretion and development of stomach lesions in swine.", "content": "In pigs treated with various doses of prednisone, pathologic changes appeared in the glandular region of the stomach, mainly in the fundic area and rarely and less severely in the cardiac and pyloric mucosae. The characteristic lesion was hemorrhagic gastritis; this was associated with massive gastric hemorrhages in pigs given the larger doses of prednisone. Epithelial changes were found in the esophageal area, but there were no ulcers. In pigs with gastric fistulas, prednisone administered at dose levels of 10 mg/kg of body weight for 8 days produced a significant increase in the volume and the acidity of gastric secretion. Hexosamine output, but not concentration, increased significantly during the treatment period. Change in pepsin secretion was sit significant. Prednisone did not produce an immediate change in gastric secretion--there being a latent period lasting 1 to 2 days before marked changes were observed. After peak changes occurred (3rd or 4th day of treatment), acidity gradually decreased to pretreatment level toward the end of the treatment period. This indicates that although increased acidity may be involved in the development of lesions in the glandular region, other factors probably have a role also in the pathogenesis of steroid-induced stomach lesions in swine.", "contents": "Effects of prednisone on gastric secretion and development of stomach lesions in swine. In pigs treated with various doses of prednisone, pathologic changes appeared in the glandular region of the stomach, mainly in the fundic area and rarely and less severely in the cardiac and pyloric mucosae. The characteristic lesion was hemorrhagic gastritis; this was associated with massive gastric hemorrhages in pigs given the larger doses of prednisone. Epithelial changes were found in the esophageal area, but there were no ulcers. In pigs with gastric fistulas, prednisone administered at dose levels of 10 mg/kg of body weight for 8 days produced a significant increase in the volume and the acidity of gastric secretion. Hexosamine output, but not concentration, increased significantly during the treatment period. Change in pepsin secretion was sit significant. Prednisone did not produce an immediate change in gastric secretion--there being a latent period lasting 1 to 2 days before marked changes were observed. After peak changes occurred (3rd or 4th day of treatment), acidity gradually decreased to pretreatment level toward the end of the treatment period. This indicates that although increased acidity may be involved in the development of lesions in the glandular region, other factors probably have a role also in the pathogenesis of steroid-induced stomach lesions in swine."}
{"id": "PMID:1090221", "title": "Activity of penicillin and novobiocin against bovine mastitis pathogens.", "content": "Penicillin (P), novobiocin (N), and both (P-N) were evaluated in vitro against 143 clinical isolates of bovine mastitis, including, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis. Greater antistaphylococcal activities were demonstrated with N alone and P-N than with P alone. Streptococcus spp were more susceptible to P alone than to N alone, and the effectiveness of P-N corresponded with that of P alone. In vitro, P-N had a wider spectrum of antibacterial activity than did either P or N. Similar results were also obtained in vivo, using the mouse protection test. The P-N gave a greater protection rate in mice experimentally infected with S aureus and Str agalactiae than did P or N.", "contents": "Activity of penicillin and novobiocin against bovine mastitis pathogens. Penicillin (P), novobiocin (N), and both (P-N) were evaluated in vitro against 143 clinical isolates of bovine mastitis, including, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis. Greater antistaphylococcal activities were demonstrated with N alone and P-N than with P alone. Streptococcus spp were more susceptible to P alone than to N alone, and the effectiveness of P-N corresponded with that of P alone. In vitro, P-N had a wider spectrum of antibacterial activity than did either P or N. Similar results were also obtained in vivo, using the mouse protection test. The P-N gave a greater protection rate in mice experimentally infected with S aureus and Str agalactiae than did P or N."}
{"id": "PMID:1090222", "title": "Diazepam and paraldehyde for treatment of severe delirium tremens. A controlled trial.", "content": "Thirty-four patients with severe delirium tremens were allocated randomly to treatment with paraldehyde (10 ml rectally very 30 minutes) or diazepam (10 mg then 5 mg intravenously every 5 minutes) until they were calm but awake. Diazepam-treated patients became calm in one half the time needed to calm patients with paraldehyde. Half of the patients had delirium tremens in association with pneumonia, pancreatitis, or alcoholic hepatitis; these patients required twice as much paraldehyde or diazepam for initial calming as patients with delirium tremens alone. Maintenance of a calm state was accomplished easily with either diazepam, intramuscularly, or paraldehyde, rectally. Adverse reactions occurred in nine patients, all of whom had been treated with paraldehyde; these patients had greater degrees of fever, tachypnea, and tachycardia and required three times longer for initial calming than patients without adverse reactions. Diazepam given under this regimen is a safe and effective sedative for management of combative patients with severe delirium tremens.", "contents": "Diazepam and paraldehyde for treatment of severe delirium tremens. A controlled trial. Thirty-four patients with severe delirium tremens were allocated randomly to treatment with paraldehyde (10 ml rectally very 30 minutes) or diazepam (10 mg then 5 mg intravenously every 5 minutes) until they were calm but awake. Diazepam-treated patients became calm in one half the time needed to calm patients with paraldehyde. Half of the patients had delirium tremens in association with pneumonia, pancreatitis, or alcoholic hepatitis; these patients required twice as much paraldehyde or diazepam for initial calming as patients with delirium tremens alone. Maintenance of a calm state was accomplished easily with either diazepam, intramuscularly, or paraldehyde, rectally. Adverse reactions occurred in nine patients, all of whom had been treated with paraldehyde; these patients had greater degrees of fever, tachypnea, and tachycardia and required three times longer for initial calming than patients without adverse reactions. Diazepam given under this regimen is a safe and effective sedative for management of combative patients with severe delirium tremens."}
{"id": "PMID:1090223", "title": "Goodpasture's syndrome: normal renal diagnostic findings.", "content": "A patient with Goodpasture's syndrome is described in whom pulmonary manifestations were dramatic, but in whom renal abnormalities were minor and only found on fluorescent and electron microscopy. His urine showed no proteinuria and no increase in cells in quantitative counts, and renal function was normal. It is suggested that there may be an indication for carrying out renal biopsies in patients with idiopathic pulmonary haemosiderosis and that this may lead to an early diagnosis of Goodpasture's syndrome.", "contents": "Goodpasture's syndrome: normal renal diagnostic findings. A patient with Goodpasture's syndrome is described in whom pulmonary manifestations were dramatic, but in whom renal abnormalities were minor and only found on fluorescent and electron microscopy. His urine showed no proteinuria and no increase in cells in quantitative counts, and renal function was normal. It is suggested that there may be an indication for carrying out renal biopsies in patients with idiopathic pulmonary haemosiderosis and that this may lead to an early diagnosis of Goodpasture's syndrome."}
{"id": "PMID:1090224", "title": "Host failure in treatment of malaria with sulfalene and pyrimethamine.", "content": "An individual infected with a multidrug-resistant strain of Plasmodium falciparum failed to respond to treatment with sulfalene and pyrimethamine. Subinoculation studies showed that parasite resistance to the drug combination was not present. Plasma levels of sulfalene and pyrimethamine in this individual were similar to those of three individuals, subinoculated from him, who were cured by the drug combination. Erythrocyte levels of sulfalene in this individual were similar to those in an individual, subinoculated from him, who was cured by the drug combination. After treatment with the drug combination, in vitro tests showed similar antimalarial activity in the serum of this individual in comparison with the serum of this individual in comparison with the serum of an individual subinoculated from him. The failure of this individual to respond to treatment with sulfalene and pyrimethamine is attributed to an undefined host factor (or factors) that appear(s) to be present in his erythrocytes.", "contents": "Host failure in treatment of malaria with sulfalene and pyrimethamine. An individual infected with a multidrug-resistant strain of Plasmodium falciparum failed to respond to treatment with sulfalene and pyrimethamine. Subinoculation studies showed that parasite resistance to the drug combination was not present. Plasma levels of sulfalene and pyrimethamine in this individual were similar to those of three individuals, subinoculated from him, who were cured by the drug combination. Erythrocyte levels of sulfalene in this individual were similar to those in an individual, subinoculated from him, who was cured by the drug combination. After treatment with the drug combination, in vitro tests showed similar antimalarial activity in the serum of this individual in comparison with the serum of this individual in comparison with the serum of an individual subinoculated from him. The failure of this individual to respond to treatment with sulfalene and pyrimethamine is attributed to an undefined host factor (or factors) that appear(s) to be present in his erythrocytes."}
{"id": "PMID:1090225", "title": "Digitalis in acute myocardial infarction: help or hazard?", "content": "The use of digitalis in acute myocardial infarction remains controversial because of disagreement regarding the electrophysiologic, hemodynamic, and metabolic risks and benefits. However, there is no convincing evidence of an increased incidence of arrhythmias following digitalis therapy. Direct measurement of left ventricle function shows improvement of impaired left ventricular performance. The increased metabolic cost of enhanced inotropy following digitalis is countered by reductions in left ventricular end-diastolic pressure and volume, especially when left ventricular failure is present. Extension of infarct size shown in the dog after administration of digitalis may occur only in that experimental model or only if cardiac failure is absent. We conclude that digitalis may be recommended following acute myocardial infarction if the usual indications, supraventricular tachyarrhythmias or cardiac failure, are present.", "contents": "Digitalis in acute myocardial infarction: help or hazard? The use of digitalis in acute myocardial infarction remains controversial because of disagreement regarding the electrophysiologic, hemodynamic, and metabolic risks and benefits. However, there is no convincing evidence of an increased incidence of arrhythmias following digitalis therapy. Direct measurement of left ventricle function shows improvement of impaired left ventricular performance. The increased metabolic cost of enhanced inotropy following digitalis is countered by reductions in left ventricular end-diastolic pressure and volume, especially when left ventricular failure is present. Extension of infarct size shown in the dog after administration of digitalis may occur only in that experimental model or only if cardiac failure is absent. We conclude that digitalis may be recommended following acute myocardial infarction if the usual indications, supraventricular tachyarrhythmias or cardiac failure, are present."}
{"id": "PMID:1090228", "title": "Guidelines for prevention of catheter-associated urinary tract infections.", "content": "The most common site of nosocomial infection, involving more than 400 000 patients in this country annually, is the catheterized urinary tract. These infections cause significant morbidity, prolong hospital stay, increase hospitalization costs, and contribute to mortality caused by Gram-negative septicemia. Strict adherence to appropriate infection control procedures is necessary if the incidence and sequels of catheter-related infections are to be reduced. Based upon current understanding of the epidemiology of nosocomial urinary tract infections, guidelines for the prevention of catheter-associated urinary tract infections are presented.", "contents": "Guidelines for prevention of catheter-associated urinary tract infections. The most common site of nosocomial infection, involving more than 400 000 patients in this country annually, is the catheterized urinary tract. These infections cause significant morbidity, prolong hospital stay, increase hospitalization costs, and contribute to mortality caused by Gram-negative septicemia. Strict adherence to appropriate infection control procedures is necessary if the incidence and sequels of catheter-related infections are to be reduced. Based upon current understanding of the epidemiology of nosocomial urinary tract infections, guidelines for the prevention of catheter-associated urinary tract infections are presented."}
{"id": "PMID:1090229", "title": "Autoimmunity: present concepts.", "content": "Autoimmune disease may be effected by humoral mechanisms (direct antibody effect or pathogenic antigen-antibody complexes), cell-mediated mechanisms, and possibly complement in the absence of antibody. In view of recent work, self-tolerance can no longer be explained by the clonal selection theory; cells that are capable of recognizing self-antigens and effecting an autoimmune response seem to be present in the normal individual but actively regulated by a process involving antigen-antibody complexes or suppressor T cells. The determination that a clinical state is an autoimmune disease cannot, therefore, be made only on the basis of the presence of autoantibody as this may be part of the normal and active state of immunoregulation of autoimmunity. Indeed, the absence of autoantibody may be the immunodeficient state that allows a full-blown autoimmune disease to occur. In view of this knowledge, the basis for the diagnosis of autoimmune disease and the rationale for treatment based on the objective of inhibiting all immune responses to the autoantigen require re-examination.", "contents": "Autoimmunity: present concepts. Autoimmune disease may be effected by humoral mechanisms (direct antibody effect or pathogenic antigen-antibody complexes), cell-mediated mechanisms, and possibly complement in the absence of antibody. In view of recent work, self-tolerance can no longer be explained by the clonal selection theory; cells that are capable of recognizing self-antigens and effecting an autoimmune response seem to be present in the normal individual but actively regulated by a process involving antigen-antibody complexes or suppressor T cells. The determination that a clinical state is an autoimmune disease cannot, therefore, be made only on the basis of the presence of autoantibody as this may be part of the normal and active state of immunoregulation of autoimmunity. Indeed, the absence of autoantibody may be the immunodeficient state that allows a full-blown autoimmune disease to occur. In view of this knowledge, the basis for the diagnosis of autoimmune disease and the rationale for treatment based on the objective of inhibiting all immune responses to the autoantigen require re-examination."}
{"id": "PMID:1090249", "title": "Detection and growth of enteropathogenic Escherichia coli in soft ripened cheese.", "content": "The organism most frequently encountered during the 1971 outbreak of enteropathogenic Escherichia coli (EPEC) in soft ripened cheese was a strain that failed to ferment lactose broth within 48 h. Since existing methods for E. coli are dependent upon fermentation of this sugar, such strains can remain undetected, particularly when present in low numbers. Therefore a cultural testing procedure was developed to insure isolation of both lactose-positive and -negative strains. This method used GN broth, modified by substituting lactose and arabinose for glucose and D-mannitol, as an enrichment medium. MacConkey agar, used as a plating medium, was modified by substituting arabinose for half the lactose. The cultural procedure was used in conjunction with a fluorescent antibody method to screen cheese for the presence of presumptive enteropathogenic E. coli. Suspected isolates were subjected to further biochemical and serological testing and identified as members of specific serogroups. These methods were used for the analysis of over 2,000 wheels of cheese; over 10% of the samples tested were found to contain strains belonging to six different serogroups associated with diarrheal diseases. No attempt was made to confirm pathogenicity by in vivo tests. Enumeration of E. coli in cheese showed that numbers increased during storage. Cheese with less than 10 organisms/g initially increased to over 10-5 at room temperature and over 10-3 at 4 C within 10 days. With higher initial counts, levels up to 10-9 were found at 4 C. These studies showed that the high levels of E. coli encountered in these products cannot be used as a direct indicator of post-processing contamination.", "contents": "Detection and growth of enteropathogenic Escherichia coli in soft ripened cheese. The organism most frequently encountered during the 1971 outbreak of enteropathogenic Escherichia coli (EPEC) in soft ripened cheese was a strain that failed to ferment lactose broth within 48 h. Since existing methods for E. coli are dependent upon fermentation of this sugar, such strains can remain undetected, particularly when present in low numbers. Therefore a cultural testing procedure was developed to insure isolation of both lactose-positive and -negative strains. This method used GN broth, modified by substituting lactose and arabinose for glucose and D-mannitol, as an enrichment medium. MacConkey agar, used as a plating medium, was modified by substituting arabinose for half the lactose. The cultural procedure was used in conjunction with a fluorescent antibody method to screen cheese for the presence of presumptive enteropathogenic E. coli. Suspected isolates were subjected to further biochemical and serological testing and identified as members of specific serogroups. These methods were used for the analysis of over 2,000 wheels of cheese; over 10% of the samples tested were found to contain strains belonging to six different serogroups associated with diarrheal diseases. No attempt was made to confirm pathogenicity by in vivo tests. Enumeration of E. coli in cheese showed that numbers increased during storage. Cheese with less than 10 organisms/g initially increased to over 10-5 at room temperature and over 10-3 at 4 C within 10 days. With higher initial counts, levels up to 10-9 were found at 4 C. These studies showed that the high levels of E. coli encountered in these products cannot be used as a direct indicator of post-processing contamination."}
{"id": "PMID:1090250", "title": "Evaluation of methods for the isolation of Salmonella and Arizona organisms from pet turtles treated with antimicrobial agents.", "content": "Turtles infected with and actively excreting Salmonella-Arizona organisms were treated with various concentrations of both Neo-Terramycin (N-Te) and Terramycin (Te) (Pfizer) for various periods of time and then tested for the presence of these pathogens by two methods, excretion and blending.  Turtles treated with 200 mug of Te per ml of container water for 9, 12, or 14 weeks, whereas when representative turtles from treatment groups were blended 72 h posttreatment these organisms were isolated from the whole turtle homogenate.  Salmonella and Arizona could be recovered from homogenate prepared from turtles treated for 7 and 14 days with 400, 800, or 1,000 mug of Te or N-Te per ml.  These findings suggest that the blending methods is more sensistive than the excretion method for the detection of Salmonella-Arizona in the treated turtle.", "contents": "Evaluation of methods for the isolation of Salmonella and Arizona organisms from pet turtles treated with antimicrobial agents. Turtles infected with and actively excreting Salmonella-Arizona organisms were treated with various concentrations of both Neo-Terramycin (N-Te) and Terramycin (Te) (Pfizer) for various periods of time and then tested for the presence of these pathogens by two methods, excretion and blending.  Turtles treated with 200 mug of Te per ml of container water for 9, 12, or 14 weeks, whereas when representative turtles from treatment groups were blended 72 h posttreatment these organisms were isolated from the whole turtle homogenate.  Salmonella and Arizona could be recovered from homogenate prepared from turtles treated for 7 and 14 days with 400, 800, or 1,000 mug of Te or N-Te per ml.  These findings suggest that the blending methods is more sensistive than the excretion method for the detection of Salmonella-Arizona in the treated turtle."}
{"id": "PMID:1090251", "title": "Proficiency test specimens for water bacteriology.", "content": "A procedure for the preparation and distribution of simulated water specimens for coliform density testing is described.  Lyophilization of Escherichia coli in a cooked meat-glucose supporting substrate provides stable samples which may be distributed to participants in a proficiency testing program.  Logarithmic conversion of the data allows statistical evaluation of the results for inter- and intralaboratory variation.  Comparisons between the most probable number and membrane filter techniques indicate no significant difference in the accuracy of these techniques, although the membrane filter technique is more precise.", "contents": "Proficiency test specimens for water bacteriology. A procedure for the preparation and distribution of simulated water specimens for coliform density testing is described.  Lyophilization of Escherichia coli in a cooked meat-glucose supporting substrate provides stable samples which may be distributed to participants in a proficiency testing program.  Logarithmic conversion of the data allows statistical evaluation of the results for inter- and intralaboratory variation.  Comparisons between the most probable number and membrane filter techniques indicate no significant difference in the accuracy of these techniques, although the membrane filter technique is more precise."}
{"id": "PMID:1090252", "title": "Length of incubation for enumerating nitrifying bacteria present in various environments.", "content": "The effect of incubation time on most-probable-number estimates of autotrophic nitrifying bacteria was investigated by using waters, rooted aquatic plants, sediments, and slimes as inoculum sources. Maximum most probable numbers of the NH4+-oxidizing group were attained in 20 to 55 days (median, 25). Estimates of NO2-oxidizers were highest at termination (103 to 113) days.", "contents": "Length of incubation for enumerating nitrifying bacteria present in various environments. The effect of incubation time on most-probable-number estimates of autotrophic nitrifying bacteria was investigated by using waters, rooted aquatic plants, sediments, and slimes as inoculum sources. Maximum most probable numbers of the NH4+-oxidizing group were attained in 20 to 55 days (median, 25). Estimates of NO2-oxidizers were highest at termination (103 to 113) days."}
{"id": "PMID:1090253", "title": "Rapid gas chromatographic technique for presumptive detection of Clostridium botulinum in contaminated food.", "content": "A simple gas-liquid chromatography end-product assay is reported for butyric and other short-chain fatty acids as presumptive indicators of Clostridium botulinum contamination in food.", "contents": "Rapid gas chromatographic technique for presumptive detection of Clostridium botulinum in contaminated food. A simple gas-liquid chromatography end-product assay is reported for butyric and other short-chain fatty acids as presumptive indicators of Clostridium botulinum contamination in food."}
{"id": "PMID:1090254", "title": "Bacteriological survey of raw beef patties produced at establishments under federal inspection.", "content": "At the time of manufacture, 76% of 74 sets of raw beef patties collected in 42 federally inspected establishments had aerobic plate counts of 1,000,000 or fewer/g; 84% contained 100 or fewer coliforms/g; 92% contained 100 or fewer Escherichia coli/g; and 85% contained 100 or fewer staphylococcus aureus/g (geometric means of 10 patties/set). Salmonellae were isolated from only three (0.4%) of 735 beef patties.", "contents": "Bacteriological survey of raw beef patties produced at establishments under federal inspection. At the time of manufacture, 76% of 74 sets of raw beef patties collected in 42 federally inspected establishments had aerobic plate counts of 1,000,000 or fewer/g; 84% contained 100 or fewer coliforms/g; 92% contained 100 or fewer Escherichia coli/g; and 85% contained 100 or fewer staphylococcus aureus/g (geometric means of 10 patties/set). Salmonellae were isolated from only three (0.4%) of 735 beef patties."}
{"id": "PMID:1090255", "title": "Effect of exposure to hyperoxic, hypobaric, and hyperbaric environments on concentrations of selected and aerobic and anaerobic fecal flora of mice.", "content": "Alterations in selected aerobic and anaerobic fecal microflora of the mouse were determined during exposure to hyperoxic and normoxic hypo- and hyperbaric environments. Examination of fecal cultures obtained during exposure for 6 weeks to either 60 or 77% oxygen concentration 1 atmosphere absolute revealed little alteration in the aerobic or anaerobic flora. There appeared to be only a retardation in the reduction of the Klebsiella-Enterobacter flora which normally occurs after weaning. During exposure to hypobaric environments (100% O-2, 0.2 atmosphere absolute), significant alterations in concentrations of Escherichia coli, slow lactose fermenters, Klebsiella-Enterobacter, and enterococci were found in some instances. All alterations were toward increased concentrations. Variations in concentrations of different colony types of obligately anaerobic gram-positive (anGPR) and gram-negative (anGNR) rods cultured during the same experiments also occurred. One colony type of anGPR appeared to decrease while a second type increased in numbers. Concentrations of three colony types of anGNR were generally, but not always, increased. During hyperbaric exposure (2.8% O-2, 7.5 atmospheres absolute), increased concentrations of Klebsiella-Enterobacter, E. coli, slow lactose fermenters and enterococci were also noted. Changes in numbers of both colony types of anGPR, when occurring, were in the direction of lower numbers. Alteration in numbers of anGNR were in both directions but were more frequent in the direction of higher numbers. After return to normal air for 4 weeks of either hypo- or hyperbaric exposure, fecal concentrations of all organisms tended to revert toward control values with the exception of the anGPR which remained in lower concentrations after termination of the hyperbaric exposure. These observations indicate that, despite the great variation in the fecal flora among individual mice, it is possible to discover the effects induced by altered gaseous environments.", "contents": "Effect of exposure to hyperoxic, hypobaric, and hyperbaric environments on concentrations of selected and aerobic and anaerobic fecal flora of mice. Alterations in selected aerobic and anaerobic fecal microflora of the mouse were determined during exposure to hyperoxic and normoxic hypo- and hyperbaric environments. Examination of fecal cultures obtained during exposure for 6 weeks to either 60 or 77% oxygen concentration 1 atmosphere absolute revealed little alteration in the aerobic or anaerobic flora. There appeared to be only a retardation in the reduction of the Klebsiella-Enterobacter flora which normally occurs after weaning. During exposure to hypobaric environments (100% O-2, 0.2 atmosphere absolute), significant alterations in concentrations of Escherichia coli, slow lactose fermenters, Klebsiella-Enterobacter, and enterococci were found in some instances. All alterations were toward increased concentrations. Variations in concentrations of different colony types of obligately anaerobic gram-positive (anGPR) and gram-negative (anGNR) rods cultured during the same experiments also occurred. One colony type of anGPR appeared to decrease while a second type increased in numbers. Concentrations of three colony types of anGNR were generally, but not always, increased. During hyperbaric exposure (2.8% O-2, 7.5 atmospheres absolute), increased concentrations of Klebsiella-Enterobacter, E. coli, slow lactose fermenters and enterococci were also noted. Changes in numbers of both colony types of anGPR, when occurring, were in the direction of lower numbers. Alteration in numbers of anGNR were in both directions but were more frequent in the direction of higher numbers. After return to normal air for 4 weeks of either hypo- or hyperbaric exposure, fecal concentrations of all organisms tended to revert toward control values with the exception of the anGPR which remained in lower concentrations after termination of the hyperbaric exposure. These observations indicate that, despite the great variation in the fecal flora among individual mice, it is possible to discover the effects induced by altered gaseous environments."}
{"id": "PMID:1090256", "title": "Bacterial survival in laundered fabrics.", "content": "Bacterial survival was determined in linens (i) inoculated with Staphylococcus auerus (ii), taken from hospital isolation patients' beds, and (iii) used by students in their homes. Two different washers using temperatures of 38, 49, 54 and 60 C, respectively, for different times were empolyed along with a commercial tumbler dryer. Findings, after macerating the linens in Waring blender and enumerating on nonselective media, indicate that acceptable levels of survivors can be acheived in motel and hotel linens by an 8- to 10-min wash cycle at 54 C followed by adequate drying. However, it is recommended that a wash cycle with 60 C for 10 to 13 min be  employed for linens in health care factilities. The microbial significance of various laundering practices is discussed.", "contents": "Bacterial survival in laundered fabrics. Bacterial survival was determined in linens (i) inoculated with Staphylococcus auerus (ii), taken from hospital isolation patients' beds, and (iii) used by students in their homes. Two different washers using temperatures of 38, 49, 54 and 60 C, respectively, for different times were empolyed along with a commercial tumbler dryer. Findings, after macerating the linens in Waring blender and enumerating on nonselective media, indicate that acceptable levels of survivors can be acheived in motel and hotel linens by an 8- to 10-min wash cycle at 54 C followed by adequate drying. However, it is recommended that a wash cycle with 60 C for 10 to 13 min be  employed for linens in health care factilities. The microbial significance of various laundering practices is discussed."}
{"id": "PMID:1090257", "title": "Bacterial flora of the hemolymph of the blue crab, Callinectes sapidus: most probable numbers.", "content": "The hemolymph of 290 freshly collected blue crabs from Chincoteague Bay, Va., was sampled over a 15-month period from August 1968 through November 1969 and most probable numbers of bacteria were determined by tube dilution. The hemolymph of 18% of all crabs sampled was found to be sterile, with 16% sterility in summer and 23% in winter samples. Despite individual variations, male crabs as a group had a higher bacterial hemolymph burden than females, and among both sexes summer counts were higher than winter. The hemolymph of crabs with missing appendages had significantly higher counts than uninjuried crabs. The annual mean hemolymph most probable numbers per ml was 2,756 for males, 1,300 for females, and 1,876 for both sexes. The higher bacterial levels found in the hemolymph of male crabs may, in part, be explained by the fact that males, which predominated in the summer samples, had a higher incidence of injury and missing appendages than did females.", "contents": "Bacterial flora of the hemolymph of the blue crab, Callinectes sapidus: most probable numbers. The hemolymph of 290 freshly collected blue crabs from Chincoteague Bay, Va., was sampled over a 15-month period from August 1968 through November 1969 and most probable numbers of bacteria were determined by tube dilution. The hemolymph of 18% of all crabs sampled was found to be sterile, with 16% sterility in summer and 23% in winter samples. Despite individual variations, male crabs as a group had a higher bacterial hemolymph burden than females, and among both sexes summer counts were higher than winter. The hemolymph of crabs with missing appendages had significantly higher counts than uninjuried crabs. The annual mean hemolymph most probable numbers per ml was 2,756 for males, 1,300 for females, and 1,876 for both sexes. The higher bacterial levels found in the hemolymph of male crabs may, in part, be explained by the fact that males, which predominated in the summer samples, had a higher incidence of injury and missing appendages than did females."}
{"id": "PMID:1090258", "title": "Bacterial flora of the hemolymph of the blue crab, Callinectes sapidus: numerical taxonomy.", "content": "Bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly Vibrio sp., Pseudomonas sp., Acinetobacter sp., Bacillus sp., Flavobacterium sp., and coliforms. Vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of May to November and was identified in up to 21% of the hemolymphs sampled.", "contents": "Bacterial flora of the hemolymph of the blue crab, Callinectes sapidus: numerical taxonomy. Bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly Vibrio sp., Pseudomonas sp., Acinetobacter sp., Bacillus sp., Flavobacterium sp., and coliforms. Vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of May to November and was identified in up to 21% of the hemolymphs sampled."}
{"id": "PMID:1090259", "title": "Prophage induction of Escherichia coli (lambda) by N-nitrosamines.", "content": "Carcinogenic N-nitrosamines were tested for their ability to induce lambda in a lysogenic strain of Escherichia coli K-12 (58-161 F+ Dimethylnitrosamine, di-n-propylnitrosamine, methyl-n-propylnitrosamine, and N-nitrosopiperidine were shown to be inducers of prophage.", "contents": "Prophage induction of Escherichia coli (lambda) by N-nitrosamines. Carcinogenic N-nitrosamines were tested for their ability to induce lambda in a lysogenic strain of Escherichia coli K-12 (58-161 F+ Dimethylnitrosamine, di-n-propylnitrosamine, methyl-n-propylnitrosamine, and N-nitrosopiperidine were shown to be inducers of prophage."}
{"id": "PMID:1090260", "title": "Livedo vasculitis (the vasculitis of atrophie blanche). Immunohistopathologic study.", "content": "Hyalinizing segmental vasculitis or livedo vasculitis (atrophie blanche) is a clinical entity with a distinctive immunohistopathologic morphology that can be distinguished from other forms of cutaneous vasculitis by histologic and direct immunofluorescent studies. Our studies showed that immunoglobulins and complement components (C-1g, C-3, and properdin) were localized in diseased vessel walls, suggesting an immune pathogenesis.", "contents": "Livedo vasculitis (the vasculitis of atrophie blanche). Immunohistopathologic study. Hyalinizing segmental vasculitis or livedo vasculitis (atrophie blanche) is a clinical entity with a distinctive immunohistopathologic morphology that can be distinguished from other forms of cutaneous vasculitis by histologic and direct immunofluorescent studies. Our studies showed that immunoglobulins and complement components (C-1g, C-3, and properdin) were localized in diseased vessel walls, suggesting an immune pathogenesis."}
{"id": "PMID:1090261", "title": "Antinuclear antibodies. II. The prognostic significance of nuclear immunofluorescent patterns in lupus erythematosus.", "content": "Nuclear immunofluorescent patterns in lupus erythematosus (LE) were correlated with the clinical picture. Patterns were classified into three groups of prognostic significance: (1) Poor-Shrunken peripheral fluorescence was found in patients with severe systemic lupus erythematosus (SLE), 60% demonstrating renal involvement. (2) Intermediate-This included the peripheral and therapy patterns, associated with 33% renal involvement in patients with SLE. (3) Good-Large and small speckle-like threads were seen in cases of very benign LE.", "contents": "Antinuclear antibodies. II. The prognostic significance of nuclear immunofluorescent patterns in lupus erythematosus. Nuclear immunofluorescent patterns in lupus erythematosus (LE) were correlated with the clinical picture. Patterns were classified into three groups of prognostic significance: (1) Poor-Shrunken peripheral fluorescence was found in patients with severe systemic lupus erythematosus (SLE), 60% demonstrating renal involvement. (2) Intermediate-This included the peripheral and therapy patterns, associated with 33% renal involvement in patients with SLE. (3) Good-Large and small speckle-like threads were seen in cases of very benign LE."}
{"id": "PMID:1090262", "title": "Human melanoma FUHS-1. Description of a new cell line.", "content": "Presented is a new established cell line, which was cultivated from a melanoma originating from a melanosis praeblastomatosa circumscripta of the leg. Recently subculture No. 105 was reached after a period of 31 month. Medium of primary culture was TC Medium Eagle spinner modified, followed from subculture No. 1 by MEM/Hanks/0.35 g/l NaHCO3. From subculture No. 6 NCTC 135 was applied simultaneously-each respective supplements. The cells grow as monolayer; absolute plating efficiency of the established cell line lies by 800/0; epithelial-like and dendritic melanin-producing cells are prominent. The number of chromosomes is considerably fluctuating; the karyotype of the established cell line lies within the range of subtriploidy. Sizewise a marker in submedian position of centromer is to be compared with one of group.", "contents": "Human melanoma FUHS-1. Description of a new cell line. Presented is a new established cell line, which was cultivated from a melanoma originating from a melanosis praeblastomatosa circumscripta of the leg. Recently subculture No. 105 was reached after a period of 31 month. Medium of primary culture was TC Medium Eagle spinner modified, followed from subculture No. 1 by MEM/Hanks/0.35 g/l NaHCO3. From subculture No. 6 NCTC 135 was applied simultaneously-each respective supplements. The cells grow as monolayer; absolute plating efficiency of the established cell line lies by 800/0; epithelial-like and dendritic melanin-producing cells are prominent. The number of chromosomes is considerably fluctuating; the karyotype of the established cell line lies within the range of subtriploidy. Sizewise a marker in submedian position of centromer is to be compared with one of group."}
{"id": "PMID:1090263", "title": "Macrophage migration inhibition test in untreated syphilis.", "content": "Two modifications of macrophage migration inhibition test, one of George and Vaughan and the other one of Svejcar, were performed on a total of 78 cases of untreated syphilis at various stages. As specific antigens were used: Treponema Pallidum ultrasonate and cardiolipin. Inhibition of migration was observed in 87 percent patients with primary syphillis and in all patients with late and late congenital syphilis. After improving and standardisation of the technique of Treponema Pallidum antigen-the migration inhibition test may be recommended as a specific in vitro-test for detection of cell mediated immunity in syphilis.", "contents": "Macrophage migration inhibition test in untreated syphilis. Two modifications of macrophage migration inhibition test, one of George and Vaughan and the other one of Svejcar, were performed on a total of 78 cases of untreated syphilis at various stages. As specific antigens were used: Treponema Pallidum ultrasonate and cardiolipin. Inhibition of migration was observed in 87 percent patients with primary syphillis and in all patients with late and late congenital syphilis. After improving and standardisation of the technique of Treponema Pallidum antigen-the migration inhibition test may be recommended as a specific in vitro-test for detection of cell mediated immunity in syphilis."}
{"id": "PMID:1090264", "title": "Chrysanthemum allergy. Pt. II: Experimental studies on the causative agents.", "content": "Experimental studies on allergic contact dermatitis due to Chrysanthemum indicum 1. (Chrysanthemum x-morifolium) (chrysanthemum of the florists) have indicated the following results: 1. In 5 patients with allergic contact dermatitis from chrysanthemum oil of turpentine and its sensitizing compounds gave no patch test responses. No relationship between contact allergy due to chrysanthemus and to turpentine oil could be determined. 2. The pyrethrins, constituents with insecticidal activity, derived from certain Chrysanthemum species and often suspected as the causative agents, play no role in chrysanthemum allergy. 3. Tests on sensitized guinea pigs (pirl bright white strain) with flowers of chrysanthemum as well as with the two sesquiterpene lactones parthenolide and alantolactone, derived from different Composite species, gave positive patch test reactions. The results showed that parthenolide produced stronger reactions than alantolactone. 4. By thin layer chromatography neither parthenolide, nor alantolactone or pyrethrosin could be detected in extracts of chrysanthemum flowers of the florists. But the investigations indicated that several other terpenic compounds are present, which gave positive color reactions to certain lactone reagents. Five of them showed strong positive patch test reactions in our patients as well as in sensitized guinea pigs. Further studies are required to identify these compounds.", "contents": "Chrysanthemum allergy. Pt. II: Experimental studies on the causative agents. Experimental studies on allergic contact dermatitis due to Chrysanthemum indicum 1. (Chrysanthemum x-morifolium) (chrysanthemum of the florists) have indicated the following results: 1. In 5 patients with allergic contact dermatitis from chrysanthemum oil of turpentine and its sensitizing compounds gave no patch test responses. No relationship between contact allergy due to chrysanthemus and to turpentine oil could be determined. 2. The pyrethrins, constituents with insecticidal activity, derived from certain Chrysanthemum species and often suspected as the causative agents, play no role in chrysanthemum allergy. 3. Tests on sensitized guinea pigs (pirl bright white strain) with flowers of chrysanthemum as well as with the two sesquiterpene lactones parthenolide and alantolactone, derived from different Composite species, gave positive patch test reactions. The results showed that parthenolide produced stronger reactions than alantolactone. 4. By thin layer chromatography neither parthenolide, nor alantolactone or pyrethrosin could be detected in extracts of chrysanthemum flowers of the florists. But the investigations indicated that several other terpenic compounds are present, which gave positive color reactions to certain lactone reagents. Five of them showed strong positive patch test reactions in our patients as well as in sensitized guinea pigs. Further studies are required to identify these compounds."}
{"id": "PMID:1090265", "title": "Humoral immune response of mice with long-term exposure to cigarette smoke.", "content": "Mice were exposed to fresh cigarette smoke for 1,17, and 38 weeks. After each period the primary and secondary humoral immune response to intraperitoneal inoculation of sheep erythrocytes was studied. Direct and indirect plaque forming cell responses in the spleen and in a pool of cervical and mediastinal lymph nodes, as well as serum hemolytic and hemagglutinating antibodies were examined. Primary plaque forming cell and antibody response to sheep erythrocytes was first enhanced and then depressed by continual cigarette smoke exposure, while the secondary response was unaffected. Serum antibody response to polyvinylpyrrolidone, an immunogen independent of thymus-derived lymphocytes, was not impaired by 41 weeks of cigarette smoke exposure.", "contents": "Humoral immune response of mice with long-term exposure to cigarette smoke. Mice were exposed to fresh cigarette smoke for 1,17, and 38 weeks. After each period the primary and secondary humoral immune response to intraperitoneal inoculation of sheep erythrocytes was studied. Direct and indirect plaque forming cell responses in the spleen and in a pool of cervical and mediastinal lymph nodes, as well as serum hemolytic and hemagglutinating antibodies were examined. Primary plaque forming cell and antibody response to sheep erythrocytes was first enhanced and then depressed by continual cigarette smoke exposure, while the secondary response was unaffected. Serum antibody response to polyvinylpyrrolidone, an immunogen independent of thymus-derived lymphocytes, was not impaired by 41 weeks of cigarette smoke exposure."}
{"id": "PMID:1090271", "title": "Cardiac surgery in patients undergoing renal dialysis or transplantation.", "content": "The coexistence of organic cardiac disease and chronic renal failure presents a therapeutic dilemma. Cardiac operations have been performed on patients who were undergoing hemodialysis or who had had successful renal transplantation. There are several aspects of management of these patients which differ from those of the routine cardiac surgical patient. Guidelines for management are outlined and discussed.", "contents": "Cardiac surgery in patients undergoing renal dialysis or transplantation. The coexistence of organic cardiac disease and chronic renal failure presents a therapeutic dilemma. Cardiac operations have been performed on patients who were undergoing hemodialysis or who had had successful renal transplantation. There are several aspects of management of these patients which differ from those of the routine cardiac surgical patient. Guidelines for management are outlined and discussed."}
{"id": "PMID:1090272", "title": "The benefits of corticosteroids in endotoxic shock.", "content": "The experiments reported here were undertaken to study the effects of pharmacological doses of corticosteroids administered alone or in conjunction with prolonged (12-hour) assisted circulation in 22 dogs subjected to LD50-60 Escherichia coli endotoxin. The most striking findings were lengthened survival time, higher cardiac output, decreased fluid requirement, and minimal evidence of pulmonary congestion or injury in the animals treated with steroids only. Unexplained mesenteric infarction prematurely terminated the experiments in animals undergoing assisted circulation. The benefits of corticosteroids in experimentally induced endotoxic shock are clearly demonstrated in these experiments. Further studies are needed to clarify the supportive role of assisted circulation in endotoxic shock and to determine any possible advantage of hypothermia over normothermia during its course.", "contents": "The benefits of corticosteroids in endotoxic shock. The experiments reported here were undertaken to study the effects of pharmacological doses of corticosteroids administered alone or in conjunction with prolonged (12-hour) assisted circulation in 22 dogs subjected to LD50-60 Escherichia coli endotoxin. The most striking findings were lengthened survival time, higher cardiac output, decreased fluid requirement, and minimal evidence of pulmonary congestion or injury in the animals treated with steroids only. Unexplained mesenteric infarction prematurely terminated the experiments in animals undergoing assisted circulation. The benefits of corticosteroids in experimentally induced endotoxic shock are clearly demonstrated in these experiments. Further studies are needed to clarify the supportive role of assisted circulation in endotoxic shock and to determine any possible advantage of hypothermia over normothermia during its course."}
{"id": "PMID:1090273", "title": "Aromatic metabolism in the fungi. Growth of Rhodotorula mucilaginosa in p-hydroxybenzoate-limited chemostats and the effects of growth rate on the synthesis of enzymes of the 3-oxoadipate pathway.", "content": "Rhodotorula mucilaginosa was grown in p-hydroxybenzoate-limited chemostats over the dilution rate (D) range 0.01 to 0.17 h-1 and growth was adequately described by the Monod theory when maintenance energy requirements were considered. The p-hydroxy-benzoate affinity constant, K8, had the relatively high value of 270 mg/I. The yield from p-hydroxbenzoate varied with dilution rate but was constant above D equal 0.07 h-1 at 0.56 g yeast/g substrate utilised. The maintenance coefficeint for growth on the aromatic substrate was 20 mg/g yeast/hr. Culture viability decreased linearly as the dilution rate was reduced. 4-Hydroxybenzoate 3-mono-oxygenase, protocatechuate 3,4-dioxygenases, 3-carboxymuconate cyclase and 3-carboxymuconolactone hydrolase activities were dilution rate-dependent, results which accord with the substrate in inducibility of these enzymes. Under carbon-limited growth conditions the addition of glucose, a catabolite repressor of these enzymes, to the aromatic medium stimulated their synthesis. Data were also obtained which indicated that whereas the synthesis of the cyclase and the hydrolase was coordinately controlled, that of the first two enzymes of the 3-oxodipate pathway was under independent control.", "contents": "Aromatic metabolism in the fungi. Growth of Rhodotorula mucilaginosa in p-hydroxybenzoate-limited chemostats and the effects of growth rate on the synthesis of enzymes of the 3-oxoadipate pathway. Rhodotorula mucilaginosa was grown in p-hydroxybenzoate-limited chemostats over the dilution rate (D) range 0.01 to 0.17 h-1 and growth was adequately described by the Monod theory when maintenance energy requirements were considered. The p-hydroxy-benzoate affinity constant, K8, had the relatively high value of 270 mg/I. The yield from p-hydroxbenzoate varied with dilution rate but was constant above D equal 0.07 h-1 at 0.56 g yeast/g substrate utilised. The maintenance coefficeint for growth on the aromatic substrate was 20 mg/g yeast/hr. Culture viability decreased linearly as the dilution rate was reduced. 4-Hydroxybenzoate 3-mono-oxygenase, protocatechuate 3,4-dioxygenases, 3-carboxymuconate cyclase and 3-carboxymuconolactone hydrolase activities were dilution rate-dependent, results which accord with the substrate in inducibility of these enzymes. Under carbon-limited growth conditions the addition of glucose, a catabolite repressor of these enzymes, to the aromatic medium stimulated their synthesis. Data were also obtained which indicated that whereas the synthesis of the cyclase and the hydrolase was coordinately controlled, that of the first two enzymes of the 3-oxodipate pathway was under independent control."}
{"id": "PMID:1090274", "title": "Accuracy of the family history method in affective illness. Comparison with direct interviews in family studies.", "content": "We interviewed available spouses and first-degree relatives of 140 bipolar and unipolar probands for current and past psychopathology, and assessed interrater reliability. Diagnoses based on direct interviews of relatives were compared with those based on reports of the probands and of all other interviewed family members. Probands underestimated the prevalence of affective illness and other psychiatric disorders in their relatives, and overestimated the age of onset of illness in their ill relatives. Probands reported more accurately about illness in their spouses and parents than in their siblings and children, but accuracy reached acceptable levels for spouses only. Diagnoses on relatives derived by combining reports of all other interviewed family members, including the proband, were slightly more accurate than those based on the proband's reports alone. Good accuracy was obtained only for reports about spouses.", "contents": "Accuracy of the family history method in affective illness. Comparison with direct interviews in family studies. We interviewed available spouses and first-degree relatives of 140 bipolar and unipolar probands for current and past psychopathology, and assessed interrater reliability. Diagnoses based on direct interviews of relatives were compared with those based on reports of the probands and of all other interviewed family members. Probands underestimated the prevalence of affective illness and other psychiatric disorders in their relatives, and overestimated the age of onset of illness in their ill relatives. Probands reported more accurately about illness in their spouses and parents than in their siblings and children, but accuracy reached acceptable levels for spouses only. Diagnoses on relatives derived by combining reports of all other interviewed family members, including the proband, were slightly more accurate than those based on the proband's reports alone. Good accuracy was obtained only for reports about spouses."}
{"id": "PMID:1090278", "title": "Heterotopic bone formation and alkaline phosphatase.", "content": "Heterotopic bone formation is osteogenesis that occurs in a part of the body that does not normally form bone. Alkaline phosphatase (orthophosphoric monoester phosphorylase) has a wide substrate specificity for catalyzing the hydrolysis of phosphate esters at an alkaline pH. The various isoenzymes are found to originate in the liver, bones, intestinal mucosa, and placenta. Numerous disorders are associated with elevations of the individual isoenzymes. The physiologic function of alkaline phosphatase is as yet unknown. The bone isoenzyme (heat labile) is found elevated in cases of heterotopic bone formation and fracture repair. Osteoblasts (bone forming cells) are unusually rich in alkaline phosphatase but the relationship of this enzyme to the process of calcification and bone formation is not clear. Alkaline phosphatase appears to be involved with the elaboration of matrix rather than with the process of mineralization in osteogenesis.", "contents": "Heterotopic bone formation and alkaline phosphatase. Heterotopic bone formation is osteogenesis that occurs in a part of the body that does not normally form bone. Alkaline phosphatase (orthophosphoric monoester phosphorylase) has a wide substrate specificity for catalyzing the hydrolysis of phosphate esters at an alkaline pH. The various isoenzymes are found to originate in the liver, bones, intestinal mucosa, and placenta. Numerous disorders are associated with elevations of the individual isoenzymes. The physiologic function of alkaline phosphatase is as yet unknown. The bone isoenzyme (heat labile) is found elevated in cases of heterotopic bone formation and fracture repair. Osteoblasts (bone forming cells) are unusually rich in alkaline phosphatase but the relationship of this enzyme to the process of calcification and bone formation is not clear. Alkaline phosphatase appears to be involved with the elaboration of matrix rather than with the process of mineralization in osteogenesis."}
{"id": "PMID:1090281", "title": "A new antireflux procedure at the esophagogastric junction: experimental and clinical evaluation.", "content": "In posterior fundoplasty, a derivative of Nissen fundoplication, the fundus is passed behind the abdominal esophagus and fixed to the anterior aspect of the stomach, thus modifying the angle of entrance of the esophagus, creating a posterior gastric reservoir relatively inaccessible to the cardia. In dogs, it was a successful as a Nissen fundoplication in reversing esophagitis caused by reflux and daily administration of histamine. In another group of dogs, a cylindrical resection of the last 4 or 6 cm of the esophagus and a posterior fundoplasty were performed simultaneously. In spite of the daily administration of 30 mg of histamine, none of the dogs developed esophagitis.  Posterior fundoplasty has proved effective in 14 patients with hiatal hernia observed for three years. In five patients undergoing a Heller myotomy for achalasia, it was used successfully to prevent postoperative regurgitation.", "contents": "A new antireflux procedure at the esophagogastric junction: experimental and clinical evaluation. In posterior fundoplasty, a derivative of Nissen fundoplication, the fundus is passed behind the abdominal esophagus and fixed to the anterior aspect of the stomach, thus modifying the angle of entrance of the esophagus, creating a posterior gastric reservoir relatively inaccessible to the cardia. In dogs, it was a successful as a Nissen fundoplication in reversing esophagitis caused by reflux and daily administration of histamine. In another group of dogs, a cylindrical resection of the last 4 or 6 cm of the esophagus and a posterior fundoplasty were performed simultaneously. In spite of the daily administration of 30 mg of histamine, none of the dogs developed esophagitis.  Posterior fundoplasty has proved effective in 14 patients with hiatal hernia observed for three years. In five patients undergoing a Heller myotomy for achalasia, it was used successfully to prevent postoperative regurgitation."}
{"id": "PMID:1090282", "title": "Acinic cell carcinoma: collective review, including bilateral cases.", "content": "Bilateral acinic cell carcinoma of the parotid gland is rare. On the basis of one case and a review of 486 parotid acinic carcinomas, unilateral as well as bilateral, total parotidectomy seems to be the treatment of choice for this low-grade malignant neoplasm that recurs commonly following inadequate excision. If involved by tumor, the facial nerve must be taken. Metastases are more likely to lung and bone than to regional nodes. For this reason, radical neck dissection is not routinely indicated.", "contents": "Acinic cell carcinoma: collective review, including bilateral cases. Bilateral acinic cell carcinoma of the parotid gland is rare. On the basis of one case and a review of 486 parotid acinic carcinomas, unilateral as well as bilateral, total parotidectomy seems to be the treatment of choice for this low-grade malignant neoplasm that recurs commonly following inadequate excision. If involved by tumor, the facial nerve must be taken. Metastases are more likely to lung and bone than to regional nodes. For this reason, radical neck dissection is not routinely indicated."}
{"id": "PMID:1090283", "title": "Antilymphocyte globulin in renal transplantation: Nephrotic syndrome and infection as possible complications.", "content": "During a two-year period, nine patients received equine antilymphocyte globulin (ALG) as part of their immunosuppressive therapy following cadaver renal transplantation. Of these, three patients (33%) developed nephrotic syndrome. A fourth patient had proteinuria greater than 3 gm/24 hr not attributable to recurrent disease or rejection. Of 32 other patients with cadaver renal allografts done during the same time, none had nephrotic syndrome and two (6%) had proteinuria greater than 3 gm/24 hr, both with biopsy evidence of rejection. This suggests an association between ALG therapy and risk for developing nephrotic syndrome, but the mechanism is not clear and deserves further investigation. During the first three months following transplantation, the nine patients treated with ALG had 20 infections, which seems to be more per patient than in our other transplant recipients.", "contents": "Antilymphocyte globulin in renal transplantation: Nephrotic syndrome and infection as possible complications. During a two-year period, nine patients received equine antilymphocyte globulin (ALG) as part of their immunosuppressive therapy following cadaver renal transplantation. Of these, three patients (33%) developed nephrotic syndrome. A fourth patient had proteinuria greater than 3 gm/24 hr not attributable to recurrent disease or rejection. Of 32 other patients with cadaver renal allografts done during the same time, none had nephrotic syndrome and two (6%) had proteinuria greater than 3 gm/24 hr, both with biopsy evidence of rejection. This suggests an association between ALG therapy and risk for developing nephrotic syndrome, but the mechanism is not clear and deserves further investigation. During the first three months following transplantation, the nine patients treated with ALG had 20 infections, which seems to be more per patient than in our other transplant recipients."}
{"id": "PMID:1090284", "title": "Electrosurgical excision of full-thickness burns.", "content": "Massive intraoperative blood loss and poor graft take have been the major problems associated with early excision and immediate grafting of full-thickness burns. By employing electrosurgery, excessive blood loss was virtually eliminated in a series of major burn excisions. Immediate graft take was excellent on electrosurgical wounds after primary burn excisions and in late reconstructive procedures. Simplicity, improved hemostasia, good graft take, and the absence of special anesthetic requirements make this method particularly applicable to the management of patients with burn injury.", "contents": "Electrosurgical excision of full-thickness burns. Massive intraoperative blood loss and poor graft take have been the major problems associated with early excision and immediate grafting of full-thickness burns. By employing electrosurgery, excessive blood loss was virtually eliminated in a series of major burn excisions. Immediate graft take was excellent on electrosurgical wounds after primary burn excisions and in late reconstructive procedures. Simplicity, improved hemostasia, good graft take, and the absence of special anesthetic requirements make this method particularly applicable to the management of patients with burn injury."}
{"id": "PMID:1090285", "title": "Intestinal necrosis and perforation after renal transplantation.", "content": "An acute intestinal disorder occurred in seven patients soon after renal transplantation. The disorder was characterized initially by massive abdominal distention with progression to multiple bowel perforations in three patients and extensive bowel necrosis in two. Surgical manipulation did not appear to be the cause since the transplants were performed retroperitoneally in the iliac fossa. Ischemia also was not a factor since the mesenteric vessels were patent with no evidence of thrombus formation or vasculitis. The cause appeared to be related to immunosuppressive agents, in particular, high doses of corticosteroids. The disease process appeared to be reversible if immunosuppression was discontinued early, as occurred in two patients.", "contents": "Intestinal necrosis and perforation after renal transplantation. An acute intestinal disorder occurred in seven patients soon after renal transplantation. The disorder was characterized initially by massive abdominal distention with progression to multiple bowel perforations in three patients and extensive bowel necrosis in two. Surgical manipulation did not appear to be the cause since the transplants were performed retroperitoneally in the iliac fossa. Ischemia also was not a factor since the mesenteric vessels were patent with no evidence of thrombus formation or vasculitis. The cause appeared to be related to immunosuppressive agents, in particular, high doses of corticosteroids. The disease process appeared to be reversible if immunosuppression was discontinued early, as occurred in two patients."}
{"id": "PMID:1090286", "title": "Transsacral repair of rectal prolapse.", "content": "Whatever the basic pathogenesis of rectal prolapse, the structural alterations invariably include a defect in the pelvic floor fascia, a deep pouch anterior to the rectum, a patulous anus, loose fixation of the rectum to the pelvic structures, and a long sigmoid colon. A variety of corrective procedures have been described for rectal prolapse, but all suffer from various disadvantages. The transsacral approach to the rectum has several advantages: (1) it is simple and safe even for the elderly, (2) it avoids an abdominal incision with its concomitant postoperative complications, (3) it will allow complete correction of all abnormalities, and (4) it has been performed with a low recurrence rate.", "contents": "Transsacral repair of rectal prolapse. Whatever the basic pathogenesis of rectal prolapse, the structural alterations invariably include a defect in the pelvic floor fascia, a deep pouch anterior to the rectum, a patulous anus, loose fixation of the rectum to the pelvic structures, and a long sigmoid colon. A variety of corrective procedures have been described for rectal prolapse, but all suffer from various disadvantages. The transsacral approach to the rectum has several advantages: (1) it is simple and safe even for the elderly, (2) it avoids an abdominal incision with its concomitant postoperative complications, (3) it will allow complete correction of all abnormalities, and (4) it has been performed with a low recurrence rate."}
{"id": "PMID:1090287", "title": "An intraluminal technique for arterial occlusion during renal transplantation.", "content": "A simple technique was developed for occlusion of atherosclerotic vessels in patients undergoing renal transplantation. Fogarty catheters were placed within the iliac arteries via a stab wound and the balloons inflated to provide safe and gentle occlusion of atherosclerotic vessels during the transplant operation.", "contents": "An intraluminal technique for arterial occlusion during renal transplantation. A simple technique was developed for occlusion of atherosclerotic vessels in patients undergoing renal transplantation. Fogarty catheters were placed within the iliac arteries via a stab wound and the balloons inflated to provide safe and gentle occlusion of atherosclerotic vessels during the transplant operation."}
{"id": "PMID:1090290", "title": "Optic nerve anomalies in basal encephalocele.", "content": "Basal encephalocele should be suspected in child when hypertelorism, defect of the bridge of the nose, and other midfacial anomalies are present. Optic nerve anomalies may occur with basal encephalocele. Those previously described are pale discs, coloboma of the optic nerve head, and pit of the optic disc. Two additional disc anomalies that are associated with basal encephalocele are megalopapilla and optic nerve dysplasia. A mass in the nose or nasopharynx under these circumstances is most likely an encephalocele and biopsy is contraindicated.", "contents": "Optic nerve anomalies in basal encephalocele. Basal encephalocele should be suspected in child when hypertelorism, defect of the bridge of the nose, and other midfacial anomalies are present. Optic nerve anomalies may occur with basal encephalocele. Those previously described are pale discs, coloboma of the optic nerve head, and pit of the optic disc. Two additional disc anomalies that are associated with basal encephalocele are megalopapilla and optic nerve dysplasia. A mass in the nose or nasopharynx under these circumstances is most likely an encephalocele and biopsy is contraindicated."}
{"id": "PMID:1090291", "title": "Posterior uveitis and primary cerebral reticulum cell sarcoma.", "content": "Primary cerebral reticulum cell sarcoma appeared as unexplained posterior uveitis. A retrospective review of 19 cases of cerebral reticulum cell sarcoma seen at Massachusetts General Hospital has not confirmed the previously described high incidence of ocular involvement in patients with this tumor. The presence of posterior uveitis remote from a definite cerebral mass should suggest the possible diagnosis of primary cerebral reticulum cell sarcoma.", "contents": "Posterior uveitis and primary cerebral reticulum cell sarcoma. Primary cerebral reticulum cell sarcoma appeared as unexplained posterior uveitis. A retrospective review of 19 cases of cerebral reticulum cell sarcoma seen at Massachusetts General Hospital has not confirmed the previously described high incidence of ocular involvement in patients with this tumor. The presence of posterior uveitis remote from a definite cerebral mass should suggest the possible diagnosis of primary cerebral reticulum cell sarcoma."}
{"id": "PMID:1090292", "title": "Reconstruction of the central one half of an eyelid.", "content": "The technique recommended in this communication concerns the use of a semicircular graft from the lateral canthal area to close a lid margin defect comprising one half of the lid. When the defect is closed, the semicircular flap will straighten and become the lateral portion of the lid.", "contents": "Reconstruction of the central one half of an eyelid. The technique recommended in this communication concerns the use of a semicircular graft from the lateral canthal area to close a lid margin defect comprising one half of the lid. When the defect is closed, the semicircular flap will straighten and become the lateral portion of the lid."}
{"id": "PMID:1090293", "title": "Eye manifestations in acrodermatitis enteropathica.", "content": "A case of acrodermatitis enteropathica is presented that is the first to manifest punctal stenosis, the fourth to show corneal changes, and the sixth to survive into adulthood.", "contents": "Eye manifestations in acrodermatitis enteropathica. A case of acrodermatitis enteropathica is presented that is the first to manifest punctal stenosis, the fourth to show corneal changes, and the sixth to survive into adulthood."}
{"id": "PMID:1090295", "title": "Behavioral studies using genetically defined mice. A bibliography.", "content": "References in this bibliography have been selected from the Subject-Strain Bibliography of Inbred Strains of Mice, maintained at The Jackson Laboratory, which attempts to include all published papers dealing with specific inbred strains of mice, named genes in mice, or named transplantable tumors. We have selected all references which appear to be of behavioral interest, including reports of the effects of neurological mutations, but have omitted genetic studies conducted with these mutants. Studies using \"white\", \"Swiss\", or undesignated mice are not included. This bibliography covers literature published from 1922 through late 1973. The authors would like to be informed of omissions, and to receive reprints of omitted papers. The bibliography is divided into three sections. The first section includes all references in which a behavioral measure appears to be the variable of primary interest. This section is divided into 16 behavioral categories: activity, aggression, audiogenic seizures, communication, emotionality, feeding, learning, maternal, memory, psychomotor, regulation, reproduction, biorhythms, sensation, social, and miscellaneous. References are assigned to a category on the basis of their apparent emphasis. The second section includes all references in which the effects of a treatment on behavior appear to be the variables of primary interest. This section is divided into nine treatment categories: age, alcohol, central nervous system, mutations, neonatal and teratogenic, population size, pharmacological agents, genetic selection, and miscellaneous. Where multiple treatments were used, references are assigned to the category of the most important treatment. The third section contains reviews and theoretical references. Each item in the bibliography is assigned to a category in one of the three sections and given a reference number. At the end of each category is a list of the reference numbers from other categories which contain information pertinent to that category. References are arranged alphabetically within each category.", "contents": "Behavioral studies using genetically defined mice. A bibliography. References in this bibliography have been selected from the Subject-Strain Bibliography of Inbred Strains of Mice, maintained at The Jackson Laboratory, which attempts to include all published papers dealing with specific inbred strains of mice, named genes in mice, or named transplantable tumors. We have selected all references which appear to be of behavioral interest, including reports of the effects of neurological mutations, but have omitted genetic studies conducted with these mutants. Studies using \"white\", \"Swiss\", or undesignated mice are not included. This bibliography covers literature published from 1922 through late 1973. The authors would like to be informed of omissions, and to receive reprints of omitted papers. The bibliography is divided into three sections. The first section includes all references in which a behavioral measure appears to be the variable of primary interest. This section is divided into 16 behavioral categories: activity, aggression, audiogenic seizures, communication, emotionality, feeding, learning, maternal, memory, psychomotor, regulation, reproduction, biorhythms, sensation, social, and miscellaneous. References are assigned to a category on the basis of their apparent emphasis. The second section includes all references in which the effects of a treatment on behavior appear to be the variables of primary interest. This section is divided into nine treatment categories: age, alcohol, central nervous system, mutations, neonatal and teratogenic, population size, pharmacological agents, genetic selection, and miscellaneous. Where multiple treatments were used, references are assigned to the category of the most important treatment. The third section contains reviews and theoretical references. Each item in the bibliography is assigned to a category in one of the three sections and given a reference number. At the end of each category is a list of the reference numbers from other categories which contain information pertinent to that category. References are arranged alphabetically within each category."}
{"id": "PMID:1090298", "title": "The action of bacterial cytidine deaminase on 5,6-dihydrocytidine.", "content": "Cytidine deaminase from Escherichia coli was found to catalyze the hydrolytic deamination of 5,6-dihydrocytidine, at a rate slightly lower than its rate of action on the normal substrate. The results suggest that nucleophilic addition by the enzyme at the 5,6 position of the substrate is not an essential part of catalysis, unless the active site is so flexible that deamination can occur with addition in one case (cytidine) and without addition in another case (5,6-dihydrocytidine). 3,4,5,6-Tetrahydrouridine bears a close structural resemblance to a hypothetical \"tetrahedral\" intermediate formed by direct water addition to 5,6-dihydrocytidine. The hydrolytic activity of the enzyme toward 5,6-dihydrocytidine and its potent inhibition by 3,4,5,6-tetrahydrouridine are presumably related by the ability of the active site to stabilize structures of this kind by tight binding. Cytidine deaminase shows no detectable activity as a catalyst for the dehydration of 6-hydroxy-5,6-dihydrouridine.", "contents": "The action of bacterial cytidine deaminase on 5,6-dihydrocytidine. Cytidine deaminase from Escherichia coli was found to catalyze the hydrolytic deamination of 5,6-dihydrocytidine, at a rate slightly lower than its rate of action on the normal substrate. The results suggest that nucleophilic addition by the enzyme at the 5,6 position of the substrate is not an essential part of catalysis, unless the active site is so flexible that deamination can occur with addition in one case (cytidine) and without addition in another case (5,6-dihydrocytidine). 3,4,5,6-Tetrahydrouridine bears a close structural resemblance to a hypothetical \"tetrahedral\" intermediate formed by direct water addition to 5,6-dihydrocytidine. The hydrolytic activity of the enzyme toward 5,6-dihydrocytidine and its potent inhibition by 3,4,5,6-tetrahydrouridine are presumably related by the ability of the active site to stabilize structures of this kind by tight binding. Cytidine deaminase shows no detectable activity as a catalyst for the dehydration of 6-hydroxy-5,6-dihydrouridine."}
{"id": "PMID:1090299", "title": "DNA-protein interactions of the rat liver non-histone chromosomal protein.", "content": "Native rat liver NHC protein-DNA interactions have been investigated by use of a nitrocellulose filter assay sensitive in detection of protein-DNA complexes. Optimal conditions for DNA-protein interactions occurs at low ionic strength conditions (110 mM phosphate buffer). A fraction of NHC proteins was enriched 25-fold by their affinity for rat DNA immobilized on cellulose columns under these conditions. At higher ionic strength (260 mM-0.04M phosphate buffer and 0.15 M sodium chloride), this fraction binds approximately sevenfold less to rat DNA but with a substantial increase in stability of the complexes. Equilibrium competition experiments indicate that at the higher ionic strength there is a considerable DNA sequence specificity of the rat DNA binding NHC protein. Since rat DNA contains three components as defined by their reassociation kinetics: single copy DNA (C0t1/2pure = 1.6 times 103); middle repetitive DNA (C0t1?1PURE = 1.1); and highly repetitive (C0t1/2pure smaller than 0.02). The two former were isolated and employed in the DNA binding assays. At the high ionic strength criterion, the rat DNA binding NHC proteins showed a substantial preference for a subset of middle repetitive DNA sequences. This suggests a preferential interaction between a class of NHC proteins and a class of middle repetitive DNA sequences.", "contents": "DNA-protein interactions of the rat liver non-histone chromosomal protein. Native rat liver NHC protein-DNA interactions have been investigated by use of a nitrocellulose filter assay sensitive in detection of protein-DNA complexes. Optimal conditions for DNA-protein interactions occurs at low ionic strength conditions (110 mM phosphate buffer). A fraction of NHC proteins was enriched 25-fold by their affinity for rat DNA immobilized on cellulose columns under these conditions. At higher ionic strength (260 mM-0.04M phosphate buffer and 0.15 M sodium chloride), this fraction binds approximately sevenfold less to rat DNA but with a substantial increase in stability of the complexes. Equilibrium competition experiments indicate that at the higher ionic strength there is a considerable DNA sequence specificity of the rat DNA binding NHC protein. Since rat DNA contains three components as defined by their reassociation kinetics: single copy DNA (C0t1/2pure = 1.6 times 103); middle repetitive DNA (C0t1?1PURE = 1.1); and highly repetitive (C0t1/2pure smaller than 0.02). The two former were isolated and employed in the DNA binding assays. At the high ionic strength criterion, the rat DNA binding NHC proteins showed a substantial preference for a subset of middle repetitive DNA sequences. This suggests a preferential interaction between a class of NHC proteins and a class of middle repetitive DNA sequences."}
{"id": "PMID:1090300", "title": "Stepwise enzymatic oligoribonucleotide synthesis including modified nucleotides.", "content": "A method has been developed for the routine synthesis of 2'(3')-o-monoacyl ribonucleoside 5'-diphosphates for stepwise synthesis of oligoribonucleotides with Escherichia coli polynucleotide phosphorylase. The use of triethyl orthoisovalerate allows the facile preparation of 2'(3')-o-isovaleryl-UDP, -CDP, -ADP, -GDP, -IDP, -EPLISON-APD, eplison-CDP, and N6-isopentenyl-ADP. The synthesis of N6-isopentenyl-ADP from ADP by N1-alkylation and the Dimroth rearrangement to N6 is reported. The effects of several factors including the nature of the divalent cation, pH, SALT CONCENTRATION, AND TIME ON THE EFFICIENCY OF THE POLYNUCLEOTIDE PHPSPHORYLASE CATALYZED SINGLE ADDITIONS OF THE 2'(3')-O-ISOVALERYL RIBONUCLEOSIDE 5'-DIPHOSPHATES TO AN OLIGORIBONUCLEOTIDE PRIMER ARE REPORTED. The syntheses of many tetranucleoside triphosphates and two pentanucleoside tetraphosphates in yields of 20-75 per cent are reported. The 2'(3')-o-isovaleryl derivatives of IDP, eplison-ADP, eplison-CDP, and N6-isopentenyl-ADP were all accepted by polynucleotide phosphorylase as substrates for the monoaddition reaction. The extension of the method to include the syntheses of oligoribonucleotides containing modified nucleosides offers a means of studying the role s of these modification by the use of relatively simple model compounds.", "contents": "Stepwise enzymatic oligoribonucleotide synthesis including modified nucleotides. A method has been developed for the routine synthesis of 2'(3')-o-monoacyl ribonucleoside 5'-diphosphates for stepwise synthesis of oligoribonucleotides with Escherichia coli polynucleotide phosphorylase. The use of triethyl orthoisovalerate allows the facile preparation of 2'(3')-o-isovaleryl-UDP, -CDP, -ADP, -GDP, -IDP, -EPLISON-APD, eplison-CDP, and N6-isopentenyl-ADP. The synthesis of N6-isopentenyl-ADP from ADP by N1-alkylation and the Dimroth rearrangement to N6 is reported. The effects of several factors including the nature of the divalent cation, pH, SALT CONCENTRATION, AND TIME ON THE EFFICIENCY OF THE POLYNUCLEOTIDE PHPSPHORYLASE CATALYZED SINGLE ADDITIONS OF THE 2'(3')-O-ISOVALERYL RIBONUCLEOSIDE 5'-DIPHOSPHATES TO AN OLIGORIBONUCLEOTIDE PRIMER ARE REPORTED. The syntheses of many tetranucleoside triphosphates and two pentanucleoside tetraphosphates in yields of 20-75 per cent are reported. The 2'(3')-o-isovaleryl derivatives of IDP, eplison-ADP, eplison-CDP, and N6-isopentenyl-ADP were all accepted by polynucleotide phosphorylase as substrates for the monoaddition reaction. The extension of the method to include the syntheses of oligoribonucleotides containing modified nucleosides offers a means of studying the role s of these modification by the use of relatively simple model compounds."}
{"id": "PMID:1090301", "title": "A permeable cell system for studying DNA replication in synchronized HeLa cells.", "content": "Treatment of HeLa cells with a hypotonic buffer solution makes them permeable to nucleotides. Cells which are in S-phase at the time of treatment continue to synthesize DNA when supplied with the four deoxyriboside triphosphates, ATP, Mg2+, and the proper ionic environment. DNA replication extends from sites which were active in the cells prior to treatment. The product is confined to the nucleus and is sensitive to deoxyribonuclease. Under optimum conditions, up to 5% of the HeLa genome can be replicated from exogenous nucleotides. In synchronized cultures the level of DNA replicase activity, as measured in permeable cells at different points in the cell cycle, correlates with the rate of [14C] thymidine incorporation measured in the living, untreated cells.", "contents": "A permeable cell system for studying DNA replication in synchronized HeLa cells. Treatment of HeLa cells with a hypotonic buffer solution makes them permeable to nucleotides. Cells which are in S-phase at the time of treatment continue to synthesize DNA when supplied with the four deoxyriboside triphosphates, ATP, Mg2+, and the proper ionic environment. DNA replication extends from sites which were active in the cells prior to treatment. The product is confined to the nucleus and is sensitive to deoxyribonuclease. Under optimum conditions, up to 5% of the HeLa genome can be replicated from exogenous nucleotides. In synchronized cultures the level of DNA replicase activity, as measured in permeable cells at different points in the cell cycle, correlates with the rate of [14C] thymidine incorporation measured in the living, untreated cells."}
{"id": "PMID:1090302", "title": "Polypeptides of a glycoprotein antigen (SF) present in serum and surface of normal but not of transformed chicken fibroblasts.", "content": "A protein immunologically identical to a glycoprotein antigen from fibroblast plasma membrane (SF antigen complex) is present in chicken serum. This antigen disappears from cells transformed with tumor viruses. The antigen solubilized from fibroblasts using urea and detergents, and the serum component both gave a molecular weight of about 2-10(5) as estimated by gel filtration on Sepharose 4B. Ultracentrifugation in 5-20% sucrose gradients gave a value of 7-8 S for the antigen from both sources. Isolation of antigen from serum and fibroblasts extracts was achieved using immunochemical techniques. Analysis of the polypeptide chains of the cellular antigen by electrophoresis in the presence of sodium dodecylsulphate, disclosed three major components at molecular weights 210 000 and 145 000 and 45 000. The two high molecular weight bands were identified in electrophoretograms of whole cell extracts by absorption to and subsequent elution from immunoadsorbents. The 45 000-molecular weight components comigrated with purified actin in electrophoresis. After short pulses of (35S) methionine the high molecular weight polypeptides, the 145 000-molecular weight component in particular, were among the most prominent radioactive bands from whole cell extracts indicating that SF antigen has a rapid turn-over. The antigen isolated from serum showed a polypeptide composition similar to the one from cells except the 45 000-molecular weight component was not detectable.", "contents": "Polypeptides of a glycoprotein antigen (SF) present in serum and surface of normal but not of transformed chicken fibroblasts. A protein immunologically identical to a glycoprotein antigen from fibroblast plasma membrane (SF antigen complex) is present in chicken serum. This antigen disappears from cells transformed with tumor viruses. The antigen solubilized from fibroblasts using urea and detergents, and the serum component both gave a molecular weight of about 2-10(5) as estimated by gel filtration on Sepharose 4B. Ultracentrifugation in 5-20% sucrose gradients gave a value of 7-8 S for the antigen from both sources. Isolation of antigen from serum and fibroblasts extracts was achieved using immunochemical techniques. Analysis of the polypeptide chains of the cellular antigen by electrophoresis in the presence of sodium dodecylsulphate, disclosed three major components at molecular weights 210 000 and 145 000 and 45 000. The two high molecular weight bands were identified in electrophoretograms of whole cell extracts by absorption to and subsequent elution from immunoadsorbents. The 45 000-molecular weight components comigrated with purified actin in electrophoresis. After short pulses of (35S) methionine the high molecular weight polypeptides, the 145 000-molecular weight component in particular, were among the most prominent radioactive bands from whole cell extracts indicating that SF antigen has a rapid turn-over. The antigen isolated from serum showed a polypeptide composition similar to the one from cells except the 45 000-molecular weight component was not detectable."}
{"id": "PMID:1090297", "title": "Azathioprine in rheumatoid arthritis: a long-term follow-up study.", "content": "In 1973 we reported the beneficial effects of azathioprine in a double blind, cross-over study in 17 patients with classic rheumatoid arthritis. During subsequent follow-up over a mean period of 40 months, 4 patients had discontinued therapy because of poor therapeutic response and 1 because of nausea. Eleven of the 12 patients still taking azathioprine had maintained their initial beneficial response or showed further improvement. Adverse side effects during the follow-up period were minor. They included nausea in 1 patient and leukopenia with thrombocytopenia in another. An increased incidence of chromosomal abnormalities was detected in those patients still receiving azathioprine.", "contents": "Azathioprine in rheumatoid arthritis: a long-term follow-up study. In 1973 we reported the beneficial effects of azathioprine in a double blind, cross-over study in 17 patients with classic rheumatoid arthritis. During subsequent follow-up over a mean period of 40 months, 4 patients had discontinued therapy because of poor therapeutic response and 1 because of nausea. Eleven of the 12 patients still taking azathioprine had maintained their initial beneficial response or showed further improvement. Adverse side effects during the follow-up period were minor. They included nausea in 1 patient and leukopenia with thrombocytopenia in another. An increased incidence of chromosomal abnormalities was detected in those patients still receiving azathioprine."}
{"id": "PMID:1090303", "title": "Polynucleotides. XXVIII. Stimulation of the binding of aminoacyl-tRNA to ribosomes by tri- and polynucleotide analogs.", "content": "Messenger activity of synthetic tri- and polynucleotide analogs was studied by binding of 14C-labeled aminoacyl-tRNAs to ribosomes in the presence of the analogs. Synthetic messengers used were: poly(A) analogs in which adenosine was replaced by tubercidine (I), 3-deazaadenosine (II), 1-deazaadenosine (III) and 2-methyladenosine (IV); copolymers of adenosine and aristeromycin (V); cyclic triadenylate (VI); the heptanucleotide of 6,2'-O-cyclouridine (VII); the pentanucleotide of 8,2'-S-cycloadenosine (VIIIa); A-U-G analogs in which adenosine was replaced by 8,2'-O- and S-cycloadenosine (VIII), 8,5'-O- and S-cycloadenosine (IX); 8-oxyadenosine (x); 8-bromoadenosine (XI) and formycine (XII). Among these oligo- and polynucleotides, analogs which contained nucleotides of anti conformation having appropriate bases for Watson-Crick type hydrogen bonding stimulated the binding of corresponding tRNAs to ribosomes.", "contents": "Polynucleotides. XXVIII. Stimulation of the binding of aminoacyl-tRNA to ribosomes by tri- and polynucleotide analogs. Messenger activity of synthetic tri- and polynucleotide analogs was studied by binding of 14C-labeled aminoacyl-tRNAs to ribosomes in the presence of the analogs. Synthetic messengers used were: poly(A) analogs in which adenosine was replaced by tubercidine (I), 3-deazaadenosine (II), 1-deazaadenosine (III) and 2-methyladenosine (IV); copolymers of adenosine and aristeromycin (V); cyclic triadenylate (VI); the heptanucleotide of 6,2'-O-cyclouridine (VII); the pentanucleotide of 8,2'-S-cycloadenosine (VIIIa); A-U-G analogs in which adenosine was replaced by 8,2'-O- and S-cycloadenosine (VIII), 8,5'-O- and S-cycloadenosine (IX); 8-oxyadenosine (x); 8-bromoadenosine (XI) and formycine (XII). Among these oligo- and polynucleotides, analogs which contained nucleotides of anti conformation having appropriate bases for Watson-Crick type hydrogen bonding stimulated the binding of corresponding tRNAs to ribosomes."}
{"id": "PMID:1090304", "title": "Steffimycin B, a DNA binding agent.", "content": "The antibiotic steffimycin B binds to double-stranded DNA as evidenced by difference spectroscopy and an increase of the thermal stability of DNA in the presence of the antibiotic. Salmon sperm DNA-steffimycin B complexes show a drastic decrease in template activity for Escherichia coli DNA polymerase I but not for DNA-idrected RNA polymerase. The differences in template properties of poly[d(A-T)] and poly (dG) - poly(dC)-antibiotic complexes,respectively, for DNA polymerase I and RNA polymerase suggest that the antibiotic interacts primarily with adenine or thymine bases or both in double-stranded DNA.", "contents": "Steffimycin B, a DNA binding agent. The antibiotic steffimycin B binds to double-stranded DNA as evidenced by difference spectroscopy and an increase of the thermal stability of DNA in the presence of the antibiotic. Salmon sperm DNA-steffimycin B complexes show a drastic decrease in template activity for Escherichia coli DNA polymerase I but not for DNA-idrected RNA polymerase. The differences in template properties of poly[d(A-T)] and poly (dG) - poly(dC)-antibiotic complexes,respectively, for DNA polymerase I and RNA polymerase suggest that the antibiotic interacts primarily with adenine or thymine bases or both in double-stranded DNA."}
{"id": "PMID:1090305", "title": "Regulation of RNA synthesis in Escherichia coli. III. Degradation of guanosine 5'-diphosphate 3'-diphosphate in cold-shocked cells.", "content": "Cold-shocked cells of Escherichia coli can degrade intracellularly accumulated guanosine 5'-diphosphate 3'-diphosphate (ppGpp). The rate of ppGpp degradation is governed, as in whole cells, by the spoT gene; a rapid breakdown reaction is associated with the presence of the spoT+ allele and at least a five-fold slower decay occurs in spoT-minus mutants. The two degradation reactions in shocked cells display the following similarities: (i) the rates of degradation are equivalent to whole cell estimates, (ii) both require a full complement of activated amino acids, (iii) both are dependent upon supplements in the reaction mixture which stimulate the availability of energy-rich compounds and (iv) neither is inhibited by concentrations of ribosomal antibiotics which severely restrict protein synthesis. Apart from characteristic rate  differences, decay of ppGpp in shocked cells derived from spoT-minus strains is discerned from spoT+ mediated decay in shocked cells by sensitivity to high concentrations of tetracycline and by manganese ion dependence.", "contents": "Regulation of RNA synthesis in Escherichia coli. III. Degradation of guanosine 5'-diphosphate 3'-diphosphate in cold-shocked cells. Cold-shocked cells of Escherichia coli can degrade intracellularly accumulated guanosine 5'-diphosphate 3'-diphosphate (ppGpp). The rate of ppGpp degradation is governed, as in whole cells, by the spoT gene; a rapid breakdown reaction is associated with the presence of the spoT+ allele and at least a five-fold slower decay occurs in spoT-minus mutants. The two degradation reactions in shocked cells display the following similarities: (i) the rates of degradation are equivalent to whole cell estimates, (ii) both require a full complement of activated amino acids, (iii) both are dependent upon supplements in the reaction mixture which stimulate the availability of energy-rich compounds and (iv) neither is inhibited by concentrations of ribosomal antibiotics which severely restrict protein synthesis. Apart from characteristic rate  differences, decay of ppGpp in shocked cells derived from spoT-minus strains is discerned from spoT+ mediated decay in shocked cells by sensitivity to high concentrations of tetracycline and by manganese ion dependence."}
{"id": "PMID:1090310", "title": "Selection of patients for bone marrow transplantation in severe aplastic anemia.", "content": "Despite androgens and intensive supportive care, satisfactory survival in severe aplastic anemia remains at 20% or less. Histocompatible bone marrow transplantation can restore normal hematopoiesis in approximately 40% of similarly severe individuals. Delay of transplantation for 3 wk after diagnosis allows time for proper evaluation and for many spontaneous recoveries. Further delay increases risks of fatal complications and decreases chances for successful transplantation while the incidence of spontaneous remission declines. When available, early histocompatible bone marrow transplantation may be the treatment of choice for severe aplastic anemia.", "contents": "Selection of patients for bone marrow transplantation in severe aplastic anemia. Despite androgens and intensive supportive care, satisfactory survival in severe aplastic anemia remains at 20% or less. Histocompatible bone marrow transplantation can restore normal hematopoiesis in approximately 40% of similarly severe individuals. Delay of transplantation for 3 wk after diagnosis allows time for proper evaluation and for many spontaneous recoveries. Further delay increases risks of fatal complications and decreases chances for successful transplantation while the incidence of spontaneous remission declines. When available, early histocompatible bone marrow transplantation may be the treatment of choice for severe aplastic anemia."}
{"id": "PMID:1090311", "title": "Culture of autologous bone marrow in diffusion chambers: effect of host irradiation.", "content": "Autologous bone marrow (BM) cells were cultured in diffusion chambers (DC) implanted into whole-body irradiated, non-irradiated, or sham-irradiated goats. Proliferation was apparent in DC implanted in both irradiated and nonirradiated goats. However, cells in DC cultured in irradiated hosts increased in number beginning earlier, proceeded at a faster rate, and reached higher numbers than in DC in nonirradiated hosts. Growth enhancement could not have occurred as a result of radiation-induced immunosuppression in autologous hosts. The nonirradiated \"target cells\" in the DC therefore constituted an indicator system for stimulatory or inhibitory substances in the host. The simultaneous increase in the number of granulocytes in peripheral blood and in DC of irradiated hosts was paralleled by an initial rise in serum colony-stimulating factor (CSF). A second, prolonged period of severe granulocytopenia following irradiation of the host correlated with high levels of serum CSF. Increased numbers of megakaryocytes were seen in DC as thrombocytopenia developed in the irradiated host. DC erythropoiesis disappeared rapidly in nonirradiated goats; however, in DC of irradiated goats, the number of erythrocytic precursors increased exponentially during ablation of host erythroid marrow. Anemia did not develop in the host during the culture period. Proliferation of mononuclear cells in DC was markedly stimulated by irradiation of the host. Proliferation of macrophages appeared independent of host treatment. These observations provide strong evidence for diffusion of specific and/or nonspecific humoral hematopoietic stimulators from the host into the DC. This stimulation appears to be elicited and/or intensified by irradiation of the host.", "contents": "Culture of autologous bone marrow in diffusion chambers: effect of host irradiation. Autologous bone marrow (BM) cells were cultured in diffusion chambers (DC) implanted into whole-body irradiated, non-irradiated, or sham-irradiated goats. Proliferation was apparent in DC implanted in both irradiated and nonirradiated goats. However, cells in DC cultured in irradiated hosts increased in number beginning earlier, proceeded at a faster rate, and reached higher numbers than in DC in nonirradiated hosts. Growth enhancement could not have occurred as a result of radiation-induced immunosuppression in autologous hosts. The nonirradiated \"target cells\" in the DC therefore constituted an indicator system for stimulatory or inhibitory substances in the host. The simultaneous increase in the number of granulocytes in peripheral blood and in DC of irradiated hosts was paralleled by an initial rise in serum colony-stimulating factor (CSF). A second, prolonged period of severe granulocytopenia following irradiation of the host correlated with high levels of serum CSF. Increased numbers of megakaryocytes were seen in DC as thrombocytopenia developed in the irradiated host. DC erythropoiesis disappeared rapidly in nonirradiated goats; however, in DC of irradiated goats, the number of erythrocytic precursors increased exponentially during ablation of host erythroid marrow. Anemia did not develop in the host during the culture period. Proliferation of mononuclear cells in DC was markedly stimulated by irradiation of the host. Proliferation of macrophages appeared independent of host treatment. These observations provide strong evidence for diffusion of specific and/or nonspecific humoral hematopoietic stimulators from the host into the DC. This stimulation appears to be elicited and/or intensified by irradiation of the host."}
{"id": "PMID:1090312", "title": "Inhibition of intravascular fibrin deposition by dipyridamole in experimental animals.", "content": "Intravascular fibrin deposition was induced in rabbits by endotoxin, the infusion of fibrin monomer (FM), and by the infusion of thrombin and EACA. A previously developed radioisotope technique was used to measure the fibrin deposits in various organs. Dipyridamole treatment of rabbits caused significant inhibition of fibrin deposition in all three experimental models. The drug also inhibited platelet consumption and, in the thrombin- and EACA-infused animals, fibrinogen consumption as well. The results obtained with dipyridamole were compared with the effect of thorotrast. It was concluded from this comparison that the effect of dipyridamole could not be attributed to inhibition of the reticuloendothelial system. It is postulated that dipyridamole inhibits the final step at which soluble FM is precipitated as fibrin in vivo.", "contents": "Inhibition of intravascular fibrin deposition by dipyridamole in experimental animals. Intravascular fibrin deposition was induced in rabbits by endotoxin, the infusion of fibrin monomer (FM), and by the infusion of thrombin and EACA. A previously developed radioisotope technique was used to measure the fibrin deposits in various organs. Dipyridamole treatment of rabbits caused significant inhibition of fibrin deposition in all three experimental models. The drug also inhibited platelet consumption and, in the thrombin- and EACA-infused animals, fibrinogen consumption as well. The results obtained with dipyridamole were compared with the effect of thorotrast. It was concluded from this comparison that the effect of dipyridamole could not be attributed to inhibition of the reticuloendothelial system. It is postulated that dipyridamole inhibits the final step at which soluble FM is precipitated as fibrin in vivo."}
{"id": "PMID:1090313", "title": "Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75.", "content": "A method of separating rosette-forming cells on columns with an immunosorbent based on sephadex G-75 is described and has been tested. The protein antigen (bovine serum albumin-BSA) is attatched by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%.", "contents": "Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75. A method of separating rosette-forming cells on columns with an immunosorbent based on sephadex G-75 is described and has been tested. The protein antigen (bovine serum albumin-BSA) is attatched by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%."}
{"id": "PMID:1090318", "title": "A comparison of one layer and two layer techniques for colorectal anastomosis.", "content": "In a randomized prospective clinical trial and layer and two layer techniques have been compared in 92 patients undergoing colorectal anastomosis. The results were assessed radiologically by barium studies on the tenth postoperative day. There was no significant difference in the incidence of anastomotic breakdown with either technique when the anastomosis was performed above the pelvic peritoneal reflection. When the anastomosis was situated below the pelvic peritoneum the incidence of dehiscence was significantly greater when a two layer technique was used.", "contents": "A comparison of one layer and two layer techniques for colorectal anastomosis. In a randomized prospective clinical trial and layer and two layer techniques have been compared in 92 patients undergoing colorectal anastomosis. The results were assessed radiologically by barium studies on the tenth postoperative day. There was no significant difference in the incidence of anastomotic breakdown with either technique when the anastomosis was performed above the pelvic peritoneal reflection. When the anastomosis was situated below the pelvic peritoneum the incidence of dehiscence was significantly greater when a two layer technique was used."}
{"id": "PMID:1090319", "title": "Cervical spinal interbody fusion with Kiel bone.", "content": "Experience with the use of the Kiel bone graft in anterior cervical interbody fusion in cervical spondylosis is reported. The survey reviews the results of operations on 73 disc spaces in 65 consecutive patients, from 2  to 5 years after the operation. We have failed to confirm that any bony fusion in the true sense occurs with Kiel bone in anterior cervical interbody fusion. However, the aims of the operations are achieved, namely removal of the disc and osteophyte, fixation of the spine to prevent compression of the neural tissues, distraction of the adjacent vertebral bodies providing increased room in the intervertebral foramina and stability of the spine.", "contents": "Cervical spinal interbody fusion with Kiel bone. Experience with the use of the Kiel bone graft in anterior cervical interbody fusion in cervical spondylosis is reported. The survey reviews the results of operations on 73 disc spaces in 65 consecutive patients, from 2  to 5 years after the operation. We have failed to confirm that any bony fusion in the true sense occurs with Kiel bone in anterior cervical interbody fusion. However, the aims of the operations are achieved, namely removal of the disc and osteophyte, fixation of the spine to prevent compression of the neural tissues, distraction of the adjacent vertebral bodies providing increased room in the intervertebral foramina and stability of the spine."}
{"id": "PMID:1090327", "title": "IgE antibodies to Dermatophagoides pteronyssinus (Housedust mite), Aspergillus fumigatus, and beta-lactoglobulin in sudden infant death syndrome.", "content": "The prevalence of serum IgE antibodies to Dermatophagoides pteronyssinus (house-dust mite), Aspergillus fumigatus, and bovine beta-lactoglobulin was significantly greater in cases of sudden infancy death (S.I.D.) than in a control group of infants of the same age range. This difference was most pronounced with D. pteronyssinus antibodies, which suggests that hypersensitivity to house-dust mite may be a factor in the aetiology of S.I.D. in Western Australia. Both the S.I.D. and control infants had similar serum concentrations of immunoglobulins G, M, and E but IgA levels were significantly higher in the control group.", "contents": "IgE antibodies to Dermatophagoides pteronyssinus (Housedust mite), Aspergillus fumigatus, and beta-lactoglobulin in sudden infant death syndrome. The prevalence of serum IgE antibodies to Dermatophagoides pteronyssinus (house-dust mite), Aspergillus fumigatus, and bovine beta-lactoglobulin was significantly greater in cases of sudden infancy death (S.I.D.) than in a control group of infants of the same age range. This difference was most pronounced with D. pteronyssinus antibodies, which suggests that hypersensitivity to house-dust mite may be a factor in the aetiology of S.I.D. in Western Australia. Both the S.I.D. and control infants had similar serum concentrations of immunoglobulins G, M, and E but IgA levels were significantly higher in the control group."}
{"id": "PMID:1090329", "title": "Effects of ambulance transport in critically ill patients.", "content": "Two groups of critically ill patients were transferred by ambulance from other hospitals to a central intensive therapy unit. The effect of transport was reviewed retrospectively in 46 patients and prospectively in 20 patients. Of the 46 patients reviewed retrospectively six became hypotensive, six became hypertensive, and seven developed delayed hypotension. One patient developed fits and six out of 13 patients had a rise in arterial PCO-2 of 1-6-4-1 kPa (12-31 mm Hg). Of the 20 patients reviewed prospectively, one patient became hypertensive due to overtransfusion, one had a fit, but none became hypotensive. Three out of four cases of delayed hypotension were related to starting intermittent positive pressure ventilation. Arterial PCO-2 fell in one patient and arterial PCO-2 rose in two, each change being related to changed oxygen therapy or narcotics. There were no changes in other cardiovascular or respiratory indices, body temperature, or urine production. Earlier transfer, resuscitation before transfer, continuing medical care during the journey, and hence a slower smoother journey seemed to be important factors in the management of these patients. Our findings, may have important implications in the future regional organization of the care of critically ill patients.", "contents": "Effects of ambulance transport in critically ill patients. Two groups of critically ill patients were transferred by ambulance from other hospitals to a central intensive therapy unit. The effect of transport was reviewed retrospectively in 46 patients and prospectively in 20 patients. Of the 46 patients reviewed retrospectively six became hypotensive, six became hypertensive, and seven developed delayed hypotension. One patient developed fits and six out of 13 patients had a rise in arterial PCO-2 of 1-6-4-1 kPa (12-31 mm Hg). Of the 20 patients reviewed prospectively, one patient became hypertensive due to overtransfusion, one had a fit, but none became hypotensive. Three out of four cases of delayed hypotension were related to starting intermittent positive pressure ventilation. Arterial PCO-2 fell in one patient and arterial PCO-2 rose in two, each change being related to changed oxygen therapy or narcotics. There were no changes in other cardiovascular or respiratory indices, body temperature, or urine production. Earlier transfer, resuscitation before transfer, continuing medical care during the journey, and hence a slower smoother journey seemed to be important factors in the management of these patients. Our findings, may have important implications in the future regional organization of the care of critically ill patients."}
{"id": "PMID:1090336", "title": "Immune complexes in cystic fibrosis.", "content": "Circulating immune complexes were detected in serum and sputum of patients with cystic fibrosis (C.F.). There were extensive deposits of immunoglobulins and complement immune complexes in several of the C.F. organs, especially the respiratory and gastrointestinal tracts, but not in the kidneys. Significant concentrations of IgG and of complement complexes could be eluted from the lungs of the C.F. patients but not from those of controls. Studies involving immunoabsorption, autoradiography, and molecular sieving through Sephadex G-200 columns identified both bovine serum albumin and staphylococcal alpha-haemolysin as two of the antigens present in the immune complexes. The sedimentation constant of the immune complexes was about 8S to 11S. The clinical significance of these immune complexes and the wide variety of antibodies detected in C.F. patients are discussed.", "contents": "Immune complexes in cystic fibrosis. Circulating immune complexes were detected in serum and sputum of patients with cystic fibrosis (C.F.). There were extensive deposits of immunoglobulins and complement immune complexes in several of the C.F. organs, especially the respiratory and gastrointestinal tracts, but not in the kidneys. Significant concentrations of IgG and of complement complexes could be eluted from the lungs of the C.F. patients but not from those of controls. Studies involving immunoabsorption, autoradiography, and molecular sieving through Sephadex G-200 columns identified both bovine serum albumin and staphylococcal alpha-haemolysin as two of the antigens present in the immune complexes. The sedimentation constant of the immune complexes was about 8S to 11S. The clinical significance of these immune complexes and the wide variety of antibodies detected in C.F. patients are discussed."}
{"id": "PMID:1090337", "title": "Comparison of aerosol ipratropium bromide and salbutamol in chronic bronchitis and asthma.", "content": "The effects of inhaling 200 mu g of salbutamol were compared with those of inhaling 40 mu g of ipratropium bromide singly and in combination with salbutamol in eight patients with bronchitis and eight asthmatic patients in a double-blind controlled trial. Changes in airways resistance were assessed by measuring the forced expiratory volume in 1 second and specific airways conductance. Both drugs were significantly better in relieving airways obstruction than placebo. Salbutamol was significantly more effective than ipratropium bromide in patients with asthma, but in the patients with bronchitis there was no significant difference between salbutamol and ipratropium bromide. The combination of the two drugs produced a slightly greater and longer response than either drug alone but this was not significant.", "contents": "Comparison of aerosol ipratropium bromide and salbutamol in chronic bronchitis and asthma. The effects of inhaling 200 mu g of salbutamol were compared with those of inhaling 40 mu g of ipratropium bromide singly and in combination with salbutamol in eight patients with bronchitis and eight asthmatic patients in a double-blind controlled trial. Changes in airways resistance were assessed by measuring the forced expiratory volume in 1 second and specific airways conductance. Both drugs were significantly better in relieving airways obstruction than placebo. Salbutamol was significantly more effective than ipratropium bromide in patients with asthma, but in the patients with bronchitis there was no significant difference between salbutamol and ipratropium bromide. The combination of the two drugs produced a slightly greater and longer response than either drug alone but this was not significant."}
{"id": "PMID:1090338", "title": "Diseases causing end-stage renal failure in New South Wales.", "content": "The nature of the original renal disease was determined in 403 consecutive cases of end-stage renal failure, in 317 of which the clinical diagnosis was corroborated by histological examination of the kidney. Five diseases accounted for 20 or more cases--glomerulonephritis (31% of the total), analgesic nephropathy (29%), primary vesicoureteral reflux (8%), essential hypertension (6%), and polycystic kidneys (5%). In only four cases did renal failure result from chronic pyelonephritis without a demonstrable primary cause. Greater use of micturating cystography and cystoscopy and routine urine testing for salicylate are advocated for earlier diagnosis of the major causes of \"pyelonephritis\". The incidence of end-stage renal failure in people aged 15-55 in New South Wales was estimated to be at least 34 new cases per million of total population each year.", "contents": "Diseases causing end-stage renal failure in New South Wales. The nature of the original renal disease was determined in 403 consecutive cases of end-stage renal failure, in 317 of which the clinical diagnosis was corroborated by histological examination of the kidney. Five diseases accounted for 20 or more cases--glomerulonephritis (31% of the total), analgesic nephropathy (29%), primary vesicoureteral reflux (8%), essential hypertension (6%), and polycystic kidneys (5%). In only four cases did renal failure result from chronic pyelonephritis without a demonstrable primary cause. Greater use of micturating cystography and cystoscopy and routine urine testing for salicylate are advocated for earlier diagnosis of the major causes of \"pyelonephritis\". The incidence of end-stage renal failure in people aged 15-55 in New South Wales was estimated to be at least 34 new cases per million of total population each year."}
{"id": "PMID:1090347", "title": "Inhibition and amplification of the radiation-induced degradation of DNA in Escherichia coli.", "content": "The degradation of DNA in a repair-proficient strain (B/r) and in two repair-defective strains (Bs-1 and pol A) of Escherichia coli was studied in the presence and absence of three metabolic inhibitors after exposure of the cells to X-radiation. The radiation-induced degradation of DNA was dependent on energy production in freshly harvested log-phase cells and was closely related to the repair capacity of the cells. A different system, probably involving nonspecific deoxyribonucleases acting at radiation-induced strand breaks, appeared to be responsible for the degradative processes in stationary-phase cells or in log-phase cells which had been aged by standing in buffered saline for 2 days before irradiation. The three inhibitors tested (caffeine, rifampin, and carbonyl cyanide m-chlorophenyl hydrazone) were all found to inhibit partially the radiation-induced degradation of DNA in E. coli cells. Under other conditions, the same three compounds amplified the degradative process. The data suggested that the amplification was due to a selective inhibition of repair processes. The degradative processes which were stimulated by X-radiation of E. coli cells show many parallels to those which are evoked by phleomycin or colicine.", "contents": "Inhibition and amplification of the radiation-induced degradation of DNA in Escherichia coli. The degradation of DNA in a repair-proficient strain (B/r) and in two repair-defective strains (Bs-1 and pol A) of Escherichia coli was studied in the presence and absence of three metabolic inhibitors after exposure of the cells to X-radiation. The radiation-induced degradation of DNA was dependent on energy production in freshly harvested log-phase cells and was closely related to the repair capacity of the cells. A different system, probably involving nonspecific deoxyribonucleases acting at radiation-induced strand breaks, appeared to be responsible for the degradative processes in stationary-phase cells or in log-phase cells which had been aged by standing in buffered saline for 2 days before irradiation. The three inhibitors tested (caffeine, rifampin, and carbonyl cyanide m-chlorophenyl hydrazone) were all found to inhibit partially the radiation-induced degradation of DNA in E. coli cells. Under other conditions, the same three compounds amplified the degradative process. The data suggested that the amplification was due to a selective inhibition of repair processes. The degradative processes which were stimulated by X-radiation of E. coli cells show many parallels to those which are evoked by phleomycin or colicine."}
{"id": "PMID:1090348", "title": "The ecological significance of sinking to planktonic bacteria.", "content": "Morphological and density measurements were made on planktonic bacteria from a subalpine lake. The observations were then used to predict in situ sinking rates from certain theoretical considerations. The predicted rates agreed well with observed sinking rates (on the order of 1 mm day-1). A simple analysis showed that sinking terms can be neglected in explaining distributions of unattached bacteria, except perhaps in relation to the presence of \"bacterial plates.\"", "contents": "The ecological significance of sinking to planktonic bacteria. Morphological and density measurements were made on planktonic bacteria from a subalpine lake. The observations were then used to predict in situ sinking rates from certain theoretical considerations. The predicted rates agreed well with observed sinking rates (on the order of 1 mm day-1). A simple analysis showed that sinking terms can be neglected in explaining distributions of unattached bacteria, except perhaps in relation to the presence of \"bacterial plates.\""}
{"id": "PMID:1090349", "title": "Lysosomes and the \"toxicity\" of Rickettsias. VI. In vivo response of mouse peritoneal phagocytes to L-cell-grown Chlamydia psittaci 6BC strain.", "content": "The L-cell-grown 6BC strain of C. psittaci inoculated intraperitoneally in mice induced an injurious effect on mononuclear phagocytes and their lysosomes; the influx of polymorphonuclear phagocytes (PMN's) increased markedly and the PMN's showed karyorrhexis and lysis. Cytochemical methods failed to detect chlamydial forms in peritoneal fluids from day 1 and up to 6 days after inoculation of mice. Chlamydial infectivity was not detected in either the cell-bound or the cell-free fractions of peritoneal cells from 6 h up to 6 days after inoculation of mice. In contrast, after infection of mice with the egg-grown 6BC strain, readily identifiable multiplication of the parasites occurred from 4 days after inoculation of mice; the lysosomes of macrophages were refractory to egg-grown 6CB particles that were initially ingested and the macrophages were nonspecifically \"activated.\" Some of these \"transformed\" into large epitheloid cells containing numerous chlamydial inclusions in the cytoplasm. That lysosomes of \"professional phagocytes\" play a role in the \"toxic\" effect of virulent chlamydiae in the intact host is discussed.", "contents": "Lysosomes and the \"toxicity\" of Rickettsias. VI. In vivo response of mouse peritoneal phagocytes to L-cell-grown Chlamydia psittaci 6BC strain. The L-cell-grown 6BC strain of C. psittaci inoculated intraperitoneally in mice induced an injurious effect on mononuclear phagocytes and their lysosomes; the influx of polymorphonuclear phagocytes (PMN's) increased markedly and the PMN's showed karyorrhexis and lysis. Cytochemical methods failed to detect chlamydial forms in peritoneal fluids from day 1 and up to 6 days after inoculation of mice. Chlamydial infectivity was not detected in either the cell-bound or the cell-free fractions of peritoneal cells from 6 h up to 6 days after inoculation of mice. In contrast, after infection of mice with the egg-grown 6BC strain, readily identifiable multiplication of the parasites occurred from 4 days after inoculation of mice; the lysosomes of macrophages were refractory to egg-grown 6CB particles that were initially ingested and the macrophages were nonspecifically \"activated.\" Some of these \"transformed\" into large epitheloid cells containing numerous chlamydial inclusions in the cytoplasm. That lysosomes of \"professional phagocytes\" play a role in the \"toxic\" effect of virulent chlamydiae in the intact host is discussed."}
{"id": "PMID:1090350", "title": "A morphological study of anaerobic bacteria from the hypolimnia of two Michigan lakes.", "content": "Dense populations of anaerobic bacteria were found sequentially layered below the thermocline in two eutrophic lakes in southwest Michigan. Phase and electron microscopy of whole cells and thin sections were used to reveal the in situ morphology of the dominant members of the community. The predominant chlorophyll-containing bacteria were identified on the basis of their morphology to be members of the genera Pelodictyon, Prosthecochloris, Clathrochloris, Chlorochromatium, Pelochromatium, Thiopedia, Thiocystis, Thiospirillum, and Chromatium. The natural morphology of these organisms is described and compared with the morphology of reported isolates, the morphology of unisolated genera was compared with previous descriptions of natural samples. Most of the organisms near the sediment-water interface and two from the upper hypolimnion have not been previously described. They have been divided into six distinct groups based on morphology; the morphological features of each group are presented. This approach, based on the morphological uniqueness of the procaryotes present, provides a satisfactory method for grouping members of the hypolimnetic community for ecological studies.", "contents": "A morphological study of anaerobic bacteria from the hypolimnia of two Michigan lakes. Dense populations of anaerobic bacteria were found sequentially layered below the thermocline in two eutrophic lakes in southwest Michigan. Phase and electron microscopy of whole cells and thin sections were used to reveal the in situ morphology of the dominant members of the community. The predominant chlorophyll-containing bacteria were identified on the basis of their morphology to be members of the genera Pelodictyon, Prosthecochloris, Clathrochloris, Chlorochromatium, Pelochromatium, Thiopedia, Thiocystis, Thiospirillum, and Chromatium. The natural morphology of these organisms is described and compared with the morphology of reported isolates, the morphology of unisolated genera was compared with previous descriptions of natural samples. Most of the organisms near the sediment-water interface and two from the upper hypolimnion have not been previously described. They have been divided into six distinct groups based on morphology; the morphological features of each group are presented. This approach, based on the morphological uniqueness of the procaryotes present, provides a satisfactory method for grouping members of the hypolimnetic community for ecological studies."}
{"id": "PMID:1090351", "title": "The structure of anaerobic bacterial communities in the hypolimnia of several Michigan lakes.", "content": "The structure of bacterial communities, the distribution of sulfide and oxygen, bacteriochlorophyll concentrations, and the temperature profile were determined for the anaerobic hypolimnia of two lakes in southern Michigan. Information from these studies, plus  qualitative observations of two other lakes and two ponds over a 4-year period were used to correlate the spatial distribution of the populations, cell size, arrangement of photosynthetic vesicles or lamellae, presence of gas vacuoles or flagella, sulfur deposition, and environmental factors. On the basis of these results, three communities designated as A, B, and C were defined. The upper (A) community consisted of sequentially layered purple sulfur bacteria including two or more of the following genera: Thiopedia, Thiospirillum, Thiocystis, or Chromatium. The middle (B) community consisted of sequential layers of green bacteria from one or more of the following genera: Pelodictyon, Clathrochloris, Chlorochromatium, or Prosthecochloris. The lowest (C)community contained previously unreported gas-vacuolate colorless bacteria 0 to 0.7 m above the sediment. Microstratification (0.1- to 0.2-m layers) of populations was observed within the A and B communities.", "contents": "The structure of anaerobic bacterial communities in the hypolimnia of several Michigan lakes. The structure of bacterial communities, the distribution of sulfide and oxygen, bacteriochlorophyll concentrations, and the temperature profile were determined for the anaerobic hypolimnia of two lakes in southern Michigan. Information from these studies, plus  qualitative observations of two other lakes and two ponds over a 4-year period were used to correlate the spatial distribution of the populations, cell size, arrangement of photosynthetic vesicles or lamellae, presence of gas vacuoles or flagella, sulfur deposition, and environmental factors. On the basis of these results, three communities designated as A, B, and C were defined. The upper (A) community consisted of sequentially layered purple sulfur bacteria including two or more of the following genera: Thiopedia, Thiospirillum, Thiocystis, or Chromatium. The middle (B) community consisted of sequential layers of green bacteria from one or more of the following genera: Pelodictyon, Clathrochloris, Chlorochromatium, or Prosthecochloris. The lowest (C)community contained previously unreported gas-vacuolate colorless bacteria 0 to 0.7 m above the sediment. Microstratification (0.1- to 0.2-m layers) of populations was observed within the A and B communities."}
{"id": "PMID:1090352", "title": "Effect of chlorpromazine on the respiration and hexose monophosphate dehydrogenases of gram-negative bacteria.", "content": "The drug chlorpromazine inhibits respiration in several gram-negative bacteria. The enzymes glucose-6-phosphogluconate dehydrogenase and 6-phosphogluconate dehydrogenase from these microorganisms are not affected by the drug.", "contents": "Effect of chlorpromazine on the respiration and hexose monophosphate dehydrogenases of gram-negative bacteria. The drug chlorpromazine inhibits respiration in several gram-negative bacteria. The enzymes glucose-6-phosphogluconate dehydrogenase and 6-phosphogluconate dehydrogenase from these microorganisms are not affected by the drug."}
{"id": "PMID:1090354", "title": "Some aspects of wound healing research: a review.", "content": "Mammalian response to injury essentially is that of tissue repair and re-epithelialization. The most important component of repair tissue is collagen, and after injury collagen turnover is greatly increased. Collagen biosynthesis is initiated by nuclear DNA of fibroblasts; the steps in biosynthesis are complex but studies of collagen biosynthesis may eventually have clinical potential. Normally, wound healing lasts for up to 2 years but nutritional and metabolic factors, such as malnutrition, delay healing; hyperalimentation would likely be beneficial under these conditions. Other factors that influence wound healing are the oxygen tension in tissues, the hemodynamic status, and the effects of substances such as cortisone, vitamins A and C, and zinc.", "contents": "Some aspects of wound healing research: a review. Mammalian response to injury essentially is that of tissue repair and re-epithelialization. The most important component of repair tissue is collagen, and after injury collagen turnover is greatly increased. Collagen biosynthesis is initiated by nuclear DNA of fibroblasts; the steps in biosynthesis are complex but studies of collagen biosynthesis may eventually have clinical potential. Normally, wound healing lasts for up to 2 years but nutritional and metabolic factors, such as malnutrition, delay healing; hyperalimentation would likely be beneficial under these conditions. Other factors that influence wound healing are the oxygen tension in tissues, the hemodynamic status, and the effects of substances such as cortisone, vitamins A and C, and zinc."}
{"id": "PMID:1090358", "title": "A double-blind comparison of intensive course 5-flourouracil by oral vs. intravenous route in the treatment of colorectal carcinoma.", "content": "This radomized double-blind study was designed to compare the therapeutic effectiveness of the oral and i.v. routes for 5-FU administered in intensive courses to 100 patients with metastatic adenocarcinoma of the large bowel, treated to equivalent levels of toxicity. An oral dose of 20 mg/kg day times 5 was found to produce comparable G.I., mucocutaneous, and hematologic side effects to a dose of 13.5 mg/kg day times 5 by rapid i.v. injection. Courses were repeated at 5 weeks. Nine of 47, or 19.1%, treated by the oral route have shown objective response, compared to 14 of 53, or 26%, treated by the i.v. route. If malignant hepatomegaly is considered alone, the response rates are 8 of 23, or 34.8%, by the oral route, and 7 of 22, or 31.8%, by the i.v. route. The mean duration of response for the oral group, 11.1 weeks, was shorter than for the i.v. route, 20 weeks, a statistically significant (p less than 0.02) difference. Serial serum 5-FU levels after two doses of 5-FU were determined by microbiological assay in 19 patients. For i.v. administration the curves were comparable among different patients as well as in the same patient. There was striking variability, however, for oral administration.", "contents": "A double-blind comparison of intensive course 5-flourouracil by oral vs. intravenous route in the treatment of colorectal carcinoma. This radomized double-blind study was designed to compare the therapeutic effectiveness of the oral and i.v. routes for 5-FU administered in intensive courses to 100 patients with metastatic adenocarcinoma of the large bowel, treated to equivalent levels of toxicity. An oral dose of 20 mg/kg day times 5 was found to produce comparable G.I., mucocutaneous, and hematologic side effects to a dose of 13.5 mg/kg day times 5 by rapid i.v. injection. Courses were repeated at 5 weeks. Nine of 47, or 19.1%, treated by the oral route have shown objective response, compared to 14 of 53, or 26%, treated by the i.v. route. If malignant hepatomegaly is considered alone, the response rates are 8 of 23, or 34.8%, by the oral route, and 7 of 22, or 31.8%, by the i.v. route. The mean duration of response for the oral group, 11.1 weeks, was shorter than for the i.v. route, 20 weeks, a statistically significant (p less than 0.02) difference. Serial serum 5-FU levels after two doses of 5-FU were determined by microbiological assay in 19 patients. For i.v. administration the curves were comparable among different patients as well as in the same patient. There was striking variability, however, for oral administration."}
{"id": "PMID:1090359", "title": "Long-term remission in diffuse histiocytic lymphoma treated with combination sequential chemotherapy.", "content": "Seventeen patients with Stage III or IV reticulum cell sarcoma were treated with three cycles of a 5-drug regimen between February, 1969 and December, 1970. Of the 17 patients, 9 attained a complete remission and 6 had a parital remission; 2 were considered unevaluable. Recently, the original biopsies from these patients were reclassified according to the criteria of rappaport et al. The results were: diffuse histiocytic--8; nodular histiocytic--2; diffuse mixed--2; nodular mixed--3; diffuse lymphocytic poorly differentiated--1; nodular lymphocytic poorly differentiated--1. Of the 8 patients with diffuse histiocytic lymphoma 6 attained complete remission, 1 had a partial remission, and 1 was unevaluable. One of the 6 with complete remission relapsed at 7 months and died 2 months later. However, the other 5 are alive and in continued unmaintained remission for 55 to 65 months.", "contents": "Long-term remission in diffuse histiocytic lymphoma treated with combination sequential chemotherapy. Seventeen patients with Stage III or IV reticulum cell sarcoma were treated with three cycles of a 5-drug regimen between February, 1969 and December, 1970. Of the 17 patients, 9 attained a complete remission and 6 had a parital remission; 2 were considered unevaluable. Recently, the original biopsies from these patients were reclassified according to the criteria of rappaport et al. The results were: diffuse histiocytic--8; nodular histiocytic--2; diffuse mixed--2; nodular mixed--3; diffuse lymphocytic poorly differentiated--1; nodular lymphocytic poorly differentiated--1. Of the 8 patients with diffuse histiocytic lymphoma 6 attained complete remission, 1 had a partial remission, and 1 was unevaluable. One of the 6 with complete remission relapsed at 7 months and died 2 months later. However, the other 5 are alive and in continued unmaintained remission for 55 to 65 months."}
{"id": "PMID:1090360", "title": "Feminizing interstitial cell tumor of the testis: personal observations and a review of the literature.", "content": "A 28-year-old man with evidence of feminization was demonstrated after 4 years of investigation to have a estrogen-secreting interstitial cell tumor. Such feminizing neoplasms are uncommon, only 37 having been described. They are usually benign and are characterized by gynecomastia, a testicular mass and, with lesser frequency, by decreased libido and potency and poor semen quality. The urinary excretion and plasma levels of estrogen are increased and, by selective testicular catheterization, the site of increased estrogen production can be localized. Secondary histologic changes occur in the nontumorous portions of the testis as well as in the contralateral testis; they are most marked in the area immediately adjacent to the tumor. Postoperatively, the gynecomastia regresses, the excessive levels of estrogen return to normal, libido improves, and the sperm count increases to normal.", "contents": "Feminizing interstitial cell tumor of the testis: personal observations and a review of the literature. A 28-year-old man with evidence of feminization was demonstrated after 4 years of investigation to have a estrogen-secreting interstitial cell tumor. Such feminizing neoplasms are uncommon, only 37 having been described. They are usually benign and are characterized by gynecomastia, a testicular mass and, with lesser frequency, by decreased libido and potency and poor semen quality. The urinary excretion and plasma levels of estrogen are increased and, by selective testicular catheterization, the site of increased estrogen production can be localized. Secondary histologic changes occur in the nontumorous portions of the testis as well as in the contralateral testis; they are most marked in the area immediately adjacent to the tumor. Postoperatively, the gynecomastia regresses, the excessive levels of estrogen return to normal, libido improves, and the sperm count increases to normal."}
{"id": "PMID:1090361", "title": "Impact of the National Cancer Act on grant support.", "content": "The National Cancer Act of 1971 resulted in a threefold increase in appropriations for the National Cancer Institute (NCI) within a 4-year period. A major effect was the increase for the Grants Program from +93 million in fiscal year 1970 to more than +280 million in 1974. Grant programs, administered by the Division of Cancer Research Resources and Centers, account for more than 50% of the total NCI extramural research budget and fall into four broad categories: research, training (including fellowships), cancer control, and construction. With the exception of the training area, funding for all grant programs has increased dramatically as a result of the Act. The ocst of research has also risen, as reflected in the average twofold increase in cost per NCI traditional grant over the past 10 years. This rise in cost is due to a number of factors, including inflation, more sophisticated equipment and supplies and, in some cases, more ambitious projects. The principal type of research grants include traditional awarded for investigator-initiated research projects, and center, awarded for comprehensive and specialized cancer centers. While support for traditional grants has remained in the forefront of NCI funding, money for cancer center grants has increased at a greater rate in recent years, reflecting emphasis on the development of cancer centers throughout the country. Compared to other institutes at the NIH, NCI is in a very favorable funding position; in fiscal year 1974 NCI awarded more money for its research grant programs than all of the other institutes (with the exception of the National Heart and Lung Institue) obligated for their entire budgets. The Act has stimulated a large increase in new cancer applications received, and the increased funding has made it possible for NCI to award a greater number of grants. Young investigators have competed well for the additional monies made available by the Act and funding for cancer research outside the United States, still only a small part of NCI's budget, has increased.", "contents": "Impact of the National Cancer Act on grant support. The National Cancer Act of 1971 resulted in a threefold increase in appropriations for the National Cancer Institute (NCI) within a 4-year period. A major effect was the increase for the Grants Program from +93 million in fiscal year 1970 to more than +280 million in 1974. Grant programs, administered by the Division of Cancer Research Resources and Centers, account for more than 50% of the total NCI extramural research budget and fall into four broad categories: research, training (including fellowships), cancer control, and construction. With the exception of the training area, funding for all grant programs has increased dramatically as a result of the Act. The ocst of research has also risen, as reflected in the average twofold increase in cost per NCI traditional grant over the past 10 years. This rise in cost is due to a number of factors, including inflation, more sophisticated equipment and supplies and, in some cases, more ambitious projects. The principal type of research grants include traditional awarded for investigator-initiated research projects, and center, awarded for comprehensive and specialized cancer centers. While support for traditional grants has remained in the forefront of NCI funding, money for cancer center grants has increased at a greater rate in recent years, reflecting emphasis on the development of cancer centers throughout the country. Compared to other institutes at the NIH, NCI is in a very favorable funding position; in fiscal year 1974 NCI awarded more money for its research grant programs than all of the other institutes (with the exception of the National Heart and Lung Institue) obligated for their entire budgets. The Act has stimulated a large increase in new cancer applications received, and the increased funding has made it possible for NCI to award a greater number of grants. Young investigators have competed well for the additional monies made available by the Act and funding for cancer research outside the United States, still only a small part of NCI's budget, has increased."}
{"id": "PMID:1090362", "title": "Quantitation of cell shedding into efferent blood of mammary adenocarcinoma.", "content": "The rate of tumor cell shedding into efferent tumor blood was measured in growing and regressing MTW9 rat mammary carcinomas. The hormone-dependent tumor, grown as an 'isoession was induced by reduction of mammotropin level in the host. Tumor cells were differentiated from normal leukocytes by indirect immunofluorescence. Growing tumors shed 3.2 x 10-6 and regressing tumors shed 4.1 x 10-6 cells per 24 hr per g tissue. Cell shedding rates of growing versus regressing tumors were not siginificantly different over a tumor size range of  2 to 4 g. The number of tumor cells in the arterial blood was 12-fold smaller than in the efferent tumor blood. It is concluded that: (ay cell shedding via blod probably plays only a minor role in the total cell loss by gtowing MTW9 carinomas; (b) hormone-induced tumor regression does not depend on increased cell shedding; (c) tumor cells are rapidly cleared from circulating blood; and (d) a 2-g MTW9 carcinoma pours enough cells into the host circulation to transplant the tumor every 24 hr.", "contents": "Quantitation of cell shedding into efferent blood of mammary adenocarcinoma. The rate of tumor cell shedding into efferent tumor blood was measured in growing and regressing MTW9 rat mammary carcinomas. The hormone-dependent tumor, grown as an 'isoession was induced by reduction of mammotropin level in the host. Tumor cells were differentiated from normal leukocytes by indirect immunofluorescence. Growing tumors shed 3.2 x 10-6 and regressing tumors shed 4.1 x 10-6 cells per 24 hr per g tissue. Cell shedding rates of growing versus regressing tumors were not siginificantly different over a tumor size range of  2 to 4 g. The number of tumor cells in the arterial blood was 12-fold smaller than in the efferent tumor blood. It is concluded that: (ay cell shedding via blod probably plays only a minor role in the total cell loss by gtowing MTW9 carinomas; (b) hormone-induced tumor regression does not depend on increased cell shedding; (c) tumor cells are rapidly cleared from circulating blood; and (d) a 2-g MTW9 carcinoma pours enough cells into the host circulation to transplant the tumor every 24 hr."}
{"id": "PMID:1090363", "title": "In vitro metabolism and microsome-mediated mutagenicity of dialkylnitrosamines in rat, hamster, and mouse tissues.", "content": "Rates of conversion of 14C-labeled dimethyl-and diethylnitrosamine by rat and hamster tissue slices to 14CO2 and/or into mutagenic reactants were measured using Salmonella typhimurium G-46 r TA 1530 and fortified tissue fractions in vitro. A correlation between the CO2 production from dimethyl- or diethylnitrosamine in liver or lung and the organ distribution of induced tumors in vivo was observed. As an exception, hamster lung, which is a major target organ in diethylnitrosamine carcinogenesis, did not convert this nitrosamine into metabolites mutagenic for S. typhimurium TA 1530 although the 14CO2 production in vitro was even higher than in hamster liver. The effect of pretreating rats, hamsters, and mice with phenobarbitone on the mutation frequency produced by dimethyl-or diethylnitrosamine in in vitro assays was determined. The relationship between the site of metabolic activation, mutagenicity, and carcinogenicity of the dialkylnitrosamines and the effect of enzyme inducers are discussed.", "contents": "In vitro metabolism and microsome-mediated mutagenicity of dialkylnitrosamines in rat, hamster, and mouse tissues. Rates of conversion of 14C-labeled dimethyl-and diethylnitrosamine by rat and hamster tissue slices to 14CO2 and/or into mutagenic reactants were measured using Salmonella typhimurium G-46 r TA 1530 and fortified tissue fractions in vitro. A correlation between the CO2 production from dimethyl- or diethylnitrosamine in liver or lung and the organ distribution of induced tumors in vivo was observed. As an exception, hamster lung, which is a major target organ in diethylnitrosamine carcinogenesis, did not convert this nitrosamine into metabolites mutagenic for S. typhimurium TA 1530 although the 14CO2 production in vitro was even higher than in hamster liver. The effect of pretreating rats, hamsters, and mice with phenobarbitone on the mutation frequency produced by dimethyl-or diethylnitrosamine in in vitro assays was determined. The relationship between the site of metabolic activation, mutagenicity, and carcinogenicity of the dialkylnitrosamines and the effect of enzyme inducers are discussed."}
{"id": "PMID:1090364", "title": "Treatment of runting syndrome and prevention of primary lymphomas in friend virus-tolerant rats.", "content": "A series of experiments showed that the inoculation of spleen and lymph node cells from rats immunized with Friend lymphoma (WFT-13) CELLS INTO Friend virus-tolerant rats induced the runting syndrome in nearly all cases, and immunological tolerance to WFT-13 was not broken in any survivors. However, th inoculation of specific immune spleen and lymph node cells admixed with normal bone marrow cells suppressed the runting death. In addition, in these animals the primary lymphomas that ordinarily occur about 200 days after neonatal inoculation of Friend virus did not appear. The mixture of immune spleen and lymph node cells and normal spleen and lymph node cells or normal thymus cells was ineffective in preventing the runting death or the incidence of primary lymphoma. Spleen and lymph node cells from normal rats or rats immunized with antigenically different AH-66 cells were also without effect. Spleen and lymph node cells from rats immunized with sheep red blood cells had a relatively high incidence of the runting syndrome; a few survivors rejected the WFT-13 transplants and also did not develop primary lymphomas. These results suggest that a supplement of hematopoietic stem cells from bone marrow eill not only prevent the runting death of Friend virus-tolerant rats produced by inoculating immune lymphoid cells but will also prevent the expected occurrence of primary lymphomas.", "contents": "Treatment of runting syndrome and prevention of primary lymphomas in friend virus-tolerant rats. A series of experiments showed that the inoculation of spleen and lymph node cells from rats immunized with Friend lymphoma (WFT-13) CELLS INTO Friend virus-tolerant rats induced the runting syndrome in nearly all cases, and immunological tolerance to WFT-13 was not broken in any survivors. However, th inoculation of specific immune spleen and lymph node cells admixed with normal bone marrow cells suppressed the runting death. In addition, in these animals the primary lymphomas that ordinarily occur about 200 days after neonatal inoculation of Friend virus did not appear. The mixture of immune spleen and lymph node cells and normal spleen and lymph node cells or normal thymus cells was ineffective in preventing the runting death or the incidence of primary lymphoma. Spleen and lymph node cells from normal rats or rats immunized with antigenically different AH-66 cells were also without effect. Spleen and lymph node cells from rats immunized with sheep red blood cells had a relatively high incidence of the runting syndrome; a few survivors rejected the WFT-13 transplants and also did not develop primary lymphomas. These results suggest that a supplement of hematopoietic stem cells from bone marrow eill not only prevent the runting death of Friend virus-tolerant rats produced by inoculating immune lymphoid cells but will also prevent the expected occurrence of primary lymphomas."}
{"id": "PMID:1090365", "title": "Epithelial and muscle antigens in benign cystic teratomas of ovary.", "content": "Thirty beign cystic teratomas of the ovary were studied by immunofluroesecence, using specific human antisera for (a) squamous epithelial intercellular areas (pemphigus sera), (b) squamous epithelial basement membranes (bullous pemphigoid sera), (c) smooth muscle (chronic active hepatitis sera, and (d) striated muscle (thymona sera). Squamous epithelial intercellular staining was seen in all specimens and was present in the basal cell layers as well as in the prickle cell layers, suggesting a slow regeneration rate for the squamous epithelial lining of these tumors. Well-formed basement membranes were identified at the periphery of all squamous epithelial structures. Smooth muscle was found in 73% of the neoplasms, and the immunoflurorescent findings correlated with light microscopy. No skeletal muscle components were identified. Within the limits of the antibodies used and the antigens detected, these resutlts support the concept that benign ovarian cystic teratomas are composed of mature, heterotopic tissue elements.", "contents": "Epithelial and muscle antigens in benign cystic teratomas of ovary. Thirty beign cystic teratomas of the ovary were studied by immunofluroesecence, using specific human antisera for (a) squamous epithelial intercellular areas (pemphigus sera), (b) squamous epithelial basement membranes (bullous pemphigoid sera), (c) smooth muscle (chronic active hepatitis sera, and (d) striated muscle (thymona sera). Squamous epithelial intercellular staining was seen in all specimens and was present in the basal cell layers as well as in the prickle cell layers, suggesting a slow regeneration rate for the squamous epithelial lining of these tumors. Well-formed basement membranes were identified at the periphery of all squamous epithelial structures. Smooth muscle was found in 73% of the neoplasms, and the immunoflurorescent findings correlated with light microscopy. No skeletal muscle components were identified. Within the limits of the antibodies used and the antigens detected, these resutlts support the concept that benign ovarian cystic teratomas are composed of mature, heterotopic tissue elements."}
{"id": "PMID:1090366", "title": "Selection for high immunogenicity in drug-resistant sublines of murine lymphomas demonstrated by plaque assay.", "content": "The immunogenicity of lymphoma L1210 and three L1210 sublines, resistant to methylglyoxal bis(guanylhydrazone), 4,4'-diacetyldiphenylurea bis(guanylhydrazone), or guanazole (L1210/GZL), respectively, was evaluated. Syngeneic DBA/2J mice were given a single i.p. injection of serially diluted suspension of irradiated cells from L1210 or L1210 sublines. Five days later spleen cells from the immunized mice were tested for the presence of plaque-forming cells using the immunizing lymphoma cell lines as target. Sera collected from the animals were examined for cytolytic antibody activity by lysis in gel using the same target cells. For comparison, the H-2 immunogenicity of L1210 and its sublines was investigated in H-2-incompatible allogeneic mice. The following results were obtained. (a) All the sublines showed increased immunogenicity and susceptibility to lysis as compared to L1210 cells. The number of plaque-forming cells/spleen ranged from 100 for L1210 to 4450 for L1210/GZL, the most immunogenic subline, and the antibody titer ranged from 1/8 for L1210 to 1/128 for L1210/GZL. (b) All the sublines carried common tumor-associated antigens that apparently made primary contributions to the increased immunogenicity. (c) The common tumor-associated antigens were also expressed on L1210 cells, although in a lesser defree, as evidenced by the definite, albeit low, capacity of L1210 cells to absorb DBA/2J anti-L1210/GZL antibodies. (d) Spleen and thymus cells of DBA/2J mice as well as unrelated murine ascites tumor cells did not cause significant absorption of these antibodies. (e) Only a partial inverse relationship could be demonstrated between tumor-associated antigens but the lowest for H-2. The above results would seem compatible with the hypothesis that the increased immunogenicity of drug-resistant L1210 sublines is attributable to the selection of preexisting highly immunogenic cells during immunosuppression by treatments selecting for drug resistance.", "contents": "Selection for high immunogenicity in drug-resistant sublines of murine lymphomas demonstrated by plaque assay. The immunogenicity of lymphoma L1210 and three L1210 sublines, resistant to methylglyoxal bis(guanylhydrazone), 4,4'-diacetyldiphenylurea bis(guanylhydrazone), or guanazole (L1210/GZL), respectively, was evaluated. Syngeneic DBA/2J mice were given a single i.p. injection of serially diluted suspension of irradiated cells from L1210 or L1210 sublines. Five days later spleen cells from the immunized mice were tested for the presence of plaque-forming cells using the immunizing lymphoma cell lines as target. Sera collected from the animals were examined for cytolytic antibody activity by lysis in gel using the same target cells. For comparison, the H-2 immunogenicity of L1210 and its sublines was investigated in H-2-incompatible allogeneic mice. The following results were obtained. (a) All the sublines showed increased immunogenicity and susceptibility to lysis as compared to L1210 cells. The number of plaque-forming cells/spleen ranged from 100 for L1210 to 4450 for L1210/GZL, the most immunogenic subline, and the antibody titer ranged from 1/8 for L1210 to 1/128 for L1210/GZL. (b) All the sublines carried common tumor-associated antigens that apparently made primary contributions to the increased immunogenicity. (c) The common tumor-associated antigens were also expressed on L1210 cells, although in a lesser defree, as evidenced by the definite, albeit low, capacity of L1210 cells to absorb DBA/2J anti-L1210/GZL antibodies. (d) Spleen and thymus cells of DBA/2J mice as well as unrelated murine ascites tumor cells did not cause significant absorption of these antibodies. (e) Only a partial inverse relationship could be demonstrated between tumor-associated antigens but the lowest for H-2. The above results would seem compatible with the hypothesis that the increased immunogenicity of drug-resistant L1210 sublines is attributable to the selection of preexisting highly immunogenic cells during immunosuppression by treatments selecting for drug resistance."}
{"id": "PMID:1090367", "title": "Comparison of the allospecific and viral-specific immune responses to irradiated versus formaldehyde-fixed allogeneic Moloney lymphoma cells in CBA mice.", "content": "Two groups of adult CBA mice were immunized with 10-7 allogeneic Moloney lymphoma (YAC) cells. These YAC (H-2a) cells, which were either irradiated with 6000 R (Group i) or were formaldehyde fixed (Group II), were injected i.p. at weekly intervals for 3 weeks. Four days following the last injection, sera and lymphocytes were collected and tested in vitro for activity against either allospecific antigens (H-2d target cells) or viral-specific antigens, namely, Moloney leukemia virus (MLV). Both groups of animals developed measurable cellular and humoral immunity to the virally determined antigens. However, only the animals in Group i, immunized with irradiated cells, developed detectable immunity to H-2d. Immune and control lymphocytes were tested in microcytotoxicity tests and by 51Cr release. Antibody was assessed by complement-dependent cytotoxicity, indirect membrane immunofluorescence, virus neutralization, and antibody-dependent lymphocyte cytotoxicity. Group I serum, which had both anti-MLV and anti-H-2 antibodies, was absorbed with either living or formaldehyde-fixed YAC cells. The living cells were able to remove both H-2 and MLV antibodies. On the other hand, the formaldehyde-fixed cells removed no H-2 antibody but were able to remove MLV antibody, although less efficiently than living cells. These data indicate that formaldehyde fixation selectively impaired the H-2 antigens, leaving the viral antigenicity relatively intact. Differences between the immune responses to MLV-determined antigens and to H-2 antigens were demonstrated in many of the parallel in vitro tests.", "contents": "Comparison of the allospecific and viral-specific immune responses to irradiated versus formaldehyde-fixed allogeneic Moloney lymphoma cells in CBA mice. Two groups of adult CBA mice were immunized with 10-7 allogeneic Moloney lymphoma (YAC) cells. These YAC (H-2a) cells, which were either irradiated with 6000 R (Group i) or were formaldehyde fixed (Group II), were injected i.p. at weekly intervals for 3 weeks. Four days following the last injection, sera and lymphocytes were collected and tested in vitro for activity against either allospecific antigens (H-2d target cells) or viral-specific antigens, namely, Moloney leukemia virus (MLV). Both groups of animals developed measurable cellular and humoral immunity to the virally determined antigens. However, only the animals in Group i, immunized with irradiated cells, developed detectable immunity to H-2d. Immune and control lymphocytes were tested in microcytotoxicity tests and by 51Cr release. Antibody was assessed by complement-dependent cytotoxicity, indirect membrane immunofluorescence, virus neutralization, and antibody-dependent lymphocyte cytotoxicity. Group I serum, which had both anti-MLV and anti-H-2 antibodies, was absorbed with either living or formaldehyde-fixed YAC cells. The living cells were able to remove both H-2 and MLV antibodies. On the other hand, the formaldehyde-fixed cells removed no H-2 antibody but were able to remove MLV antibody, although less efficiently than living cells. These data indicate that formaldehyde fixation selectively impaired the H-2 antigens, leaving the viral antigenicity relatively intact. Differences between the immune responses to MLV-determined antigens and to H-2 antigens were demonstrated in many of the parallel in vitro tests."}
{"id": "PMID:1090389", "title": "Intermolecular linking and fragmentation of DNA by beta-propiolactone, a monoalkylating carcinogen.", "content": "Brief exposure to beta-propiolactone (BPL) increases the sedimentation rate of purified Escherichia coli DNA in neutral and alkaline sucrose gradients. However, when electrophoresed in polyacrylamide-agarose gels, this BPL-treated DNA moves ahead of the control. Longer incubation with BPL gives rise to two new fractions, the first one sedimenting as a heterogeneous material of 6-8S, and the second one of very high sedimentation velocity. In acrylamide-agarose gels, the first fraction is again recovered in the 6-8S area, while the second fraction does not enter the gel at all. The DNA at this stage is hyperchromic in ultraviolet light suggesting that as much as 20% may be denaturated. Coliphage lambda DNA treated briefly with BPL and spread in a protein monolayer appears under the electron microscope as a rigid, extended molecule, up to 15% longer than the control DNA, and usually in compact, folded configurations suggesting intramolecular linking. After longer exposure, localized denaturation associated with single-strand breaks is observed. The single-stranded \"whiskers\" then interact with other DNA molecules, creating highly complex branched networks of single- and multi-stranded DNA. The possible relevance of these observations to the mechanisms involved in carcinogenesis and mutagenesis is considered.", "contents": "Intermolecular linking and fragmentation of DNA by beta-propiolactone, a monoalkylating carcinogen. Brief exposure to beta-propiolactone (BPL) increases the sedimentation rate of purified Escherichia coli DNA in neutral and alkaline sucrose gradients. However, when electrophoresed in polyacrylamide-agarose gels, this BPL-treated DNA moves ahead of the control. Longer incubation with BPL gives rise to two new fractions, the first one sedimenting as a heterogeneous material of 6-8S, and the second one of very high sedimentation velocity. In acrylamide-agarose gels, the first fraction is again recovered in the 6-8S area, while the second fraction does not enter the gel at all. The DNA at this stage is hyperchromic in ultraviolet light suggesting that as much as 20% may be denaturated. Coliphage lambda DNA treated briefly with BPL and spread in a protein monolayer appears under the electron microscope as a rigid, extended molecule, up to 15% longer than the control DNA, and usually in compact, folded configurations suggesting intramolecular linking. After longer exposure, localized denaturation associated with single-strand breaks is observed. The single-stranded \"whiskers\" then interact with other DNA molecules, creating highly complex branched networks of single- and multi-stranded DNA. The possible relevance of these observations to the mechanisms involved in carcinogenesis and mutagenesis is considered."}
{"id": "PMID:1090390", "title": "Red cell carriage of label: its limiting effect on the exchange of materials in the liver.", "content": "The red cell membrane is a permeability barrier that limits the equilibration of a variety of solutes between red cell and plasma water. We utilized the multiple indicator dilution technique to investigate the effect of this barrier on the exchange in the liver of a group of tracer substances that are not removed in net fashion from the hepatic circulation: thiourea, urea, and chloride. We demonstrated that, after preequilibration of the label with red cells, a red cell carriage effect appeared (the trapping and translocation of label in the red cells), that this effect was most marked when the permeability of the red cell was relatively low for the substance under consideration (thiourea), and that the effect became small when the permeability of the red cells was large for the exchanging substance (urea and chloride). We developed a theoretical description of the retarding effect of the red cell permeability barrier on the extravascular exchange of label and were able to use this description to obtain estimates of the red cell permeability from the in vivo dilution curves. We examined the effect of plasma injection, of changing the input in such a fashion that the label was not preequilibrated with red cells, and found both experimentally and theoretically, that for substances of low permeability the transit time from these experiments, if multiplied by the total water flow or solute flux, gave an overestimate of both the apparent total volume of distribution and the mass of traced material in the system. This last effect is of great importance for the practical design of many biological experiments. Reliable volume and mass estimates can be made only when the labeled material has been preequilibrated with red cells.", "contents": "Red cell carriage of label: its limiting effect on the exchange of materials in the liver. The red cell membrane is a permeability barrier that limits the equilibration of a variety of solutes between red cell and plasma water. We utilized the multiple indicator dilution technique to investigate the effect of this barrier on the exchange in the liver of a group of tracer substances that are not removed in net fashion from the hepatic circulation: thiourea, urea, and chloride. We demonstrated that, after preequilibration of the label with red cells, a red cell carriage effect appeared (the trapping and translocation of label in the red cells), that this effect was most marked when the permeability of the red cell was relatively low for the substance under consideration (thiourea), and that the effect became small when the permeability of the red cells was large for the exchanging substance (urea and chloride). We developed a theoretical description of the retarding effect of the red cell permeability barrier on the extravascular exchange of label and were able to use this description to obtain estimates of the red cell permeability from the in vivo dilution curves. We examined the effect of plasma injection, of changing the input in such a fashion that the label was not preequilibrated with red cells, and found both experimentally and theoretically, that for substances of low permeability the transit time from these experiments, if multiplied by the total water flow or solute flux, gave an overestimate of both the apparent total volume of distribution and the mass of traced material in the system. This last effect is of great importance for the practical design of many biological experiments. Reliable volume and mass estimates can be made only when the labeled material has been preequilibrated with red cells."}
{"id": "PMID:1090391", "title": "Red cell permeability effect on the mean transit of an indicator transported through an organ by red cells and plasma.", "content": "When an indicator that permeates red blood cells with an equilibration time of the order of the mean transit time of the indicator through an organ is bolus injected solely into the plasma inflow to that organ, the mean transit time of the indicator is greater than it is when the indicator is injected preequilibrated between the plasma inflow and the red cell inflow. The mean transit time for equilibrated entry is in turn greater than that  for indicator injected solely into the red cell inflow. A simplified method of calculation of this effect is given in the present paper; it requires only a model solution for the indicator steady state as distinguished from a more complicated time-dependent solution. The dependence of the effect on red cell membrane permeability allows this permeability to suitable indicators to be deduced. The method was applied to existing data on urea and thiourea passage through the dog kidney.", "contents": "Red cell permeability effect on the mean transit of an indicator transported through an organ by red cells and plasma. When an indicator that permeates red blood cells with an equilibration time of the order of the mean transit time of the indicator through an organ is bolus injected solely into the plasma inflow to that organ, the mean transit time of the indicator is greater than it is when the indicator is injected preequilibrated between the plasma inflow and the red cell inflow. The mean transit time for equilibrated entry is in turn greater than that  for indicator injected solely into the red cell inflow. A simplified method of calculation of this effect is given in the present paper; it requires only a model solution for the indicator steady state as distinguished from a more complicated time-dependent solution. The dependence of the effect on red cell membrane permeability allows this permeability to suitable indicators to be deduced. The method was applied to existing data on urea and thiourea passage through the dog kidney."}
{"id": "PMID:1090392", "title": "Echocardiographic features of total anomalous pulmonary venous connection.", "content": "Seven patients, four months to ten years of age, with total anomalous pulmonary venous connection (TAPVC) were studied by echocardiography.  The diagnosis was confirmed by cardiac catheterization, angiography, and surgery in each.  The connection was to the left vertical vein in four patients, to the coronary sinus in one, to the inferior vena cava in one, and directly to the right atrium in one.  All patients exhibited the echocardiographic criteria of right ventricular diastolic volume overload (RVDVO).  In addition, an echo-free space was identified dorsal to the posterior wall of the left atrium.  Indocyanine green dye studies done in one of the patients provided evidence that this echo-free space represents the common pulmonary venous chamber.  The combination of echocardiographic evidence of RVDVO and demonstration of an echo-free space dorsal to the left atrium is strongly suggestive of TAPVC and should allow an early diagnosis in infants with this anomaly.", "contents": "Echocardiographic features of total anomalous pulmonary venous connection. Seven patients, four months to ten years of age, with total anomalous pulmonary venous connection (TAPVC) were studied by echocardiography.  The diagnosis was confirmed by cardiac catheterization, angiography, and surgery in each.  The connection was to the left vertical vein in four patients, to the coronary sinus in one, to the inferior vena cava in one, and directly to the right atrium in one.  All patients exhibited the echocardiographic criteria of right ventricular diastolic volume overload (RVDVO).  In addition, an echo-free space was identified dorsal to the posterior wall of the left atrium.  Indocyanine green dye studies done in one of the patients provided evidence that this echo-free space represents the common pulmonary venous chamber.  The combination of echocardiographic evidence of RVDVO and demonstration of an echo-free space dorsal to the left atrium is strongly suggestive of TAPVC and should allow an early diagnosis in infants with this anomaly."}
{"id": "PMID:1090393", "title": "Circulatory and metabolic effects of glycerol infusion in patients with recent cerebral infarction.", "content": "The effect of intravenous infusion of 10 per cent glycerol on regional cerebral blood flow (using hydrogen bolus and Xenon-133 (133Xe) clearance methods) and metabolism was investigated in 57 patients with recent cerebral infarction. Hemispheric blood flow (HBF) increased, together with increase in regional cerebral blood flow (rCBF) and cerebral blood volume (rCBV), in foci of brain ischemia. Hemispheric oxygen consumption (HMIO2) decreased together with hemispheric respiratory quotient. Systemic blood levels of glucose, lactate, pyruvate, and triglycerides also increased after glycerol while free fatty acids (FFA) and inorganic phosphate (Pi) decreased. Hemispheric glucose consumption was unaltered after glycerol so that hemispheric glucose to oxygen ratio tended to rise. Pyruvate and lactate production by brain was unchanged. Glycerol moved across the blood brain barrier into brain and cerebrospinal fluid (CSF). Release of FFA and Pi from infarcted brain was reversed by glycerol. Total phosphate balance was maintained actoss brain both before and after glycerol infusion. Triglycerides increased in CSF after glycerol, originating either from cerebral blood or as a result of lipogenesis in cerebral tissue. The EEG Recording and neurological status of the patients improved despite decreased brain oxygen consumption. Results of this study suggest that after intravenous infusion of 10 per cent glycerol in patients with recent cerebral infarction, glycerol rapidly enters the CSF and brain compartments and favorably affects the stroke process in two ways: first, by redistribution of cerebral blood flow with increase in rCBF and rCBV in ischemic brain secondary to reduction in focal cerebral edema; and second glycerol may become an alternative source of energy either by being directly metabolized by the brain, or indirectly, by enhancing lipogenesis, or by both processes. Involvement of glycerol in lipogenesis with esterification to accumulated FFA might lead to improved coupling of oxidative phosphorylation, a hypothesis that fits the finding of improved neuronal function despite further decrease in cerebral hemispheric oxygen consumption.", "contents": "Circulatory and metabolic effects of glycerol infusion in patients with recent cerebral infarction. The effect of intravenous infusion of 10 per cent glycerol on regional cerebral blood flow (using hydrogen bolus and Xenon-133 (133Xe) clearance methods) and metabolism was investigated in 57 patients with recent cerebral infarction. Hemispheric blood flow (HBF) increased, together with increase in regional cerebral blood flow (rCBF) and cerebral blood volume (rCBV), in foci of brain ischemia. Hemispheric oxygen consumption (HMIO2) decreased together with hemispheric respiratory quotient. Systemic blood levels of glucose, lactate, pyruvate, and triglycerides also increased after glycerol while free fatty acids (FFA) and inorganic phosphate (Pi) decreased. Hemispheric glucose consumption was unaltered after glycerol so that hemispheric glucose to oxygen ratio tended to rise. Pyruvate and lactate production by brain was unchanged. Glycerol moved across the blood brain barrier into brain and cerebrospinal fluid (CSF). Release of FFA and Pi from infarcted brain was reversed by glycerol. Total phosphate balance was maintained actoss brain both before and after glycerol infusion. Triglycerides increased in CSF after glycerol, originating either from cerebral blood or as a result of lipogenesis in cerebral tissue. The EEG Recording and neurological status of the patients improved despite decreased brain oxygen consumption. Results of this study suggest that after intravenous infusion of 10 per cent glycerol in patients with recent cerebral infarction, glycerol rapidly enters the CSF and brain compartments and favorably affects the stroke process in two ways: first, by redistribution of cerebral blood flow with increase in rCBF and rCBV in ischemic brain secondary to reduction in focal cerebral edema; and second glycerol may become an alternative source of energy either by being directly metabolized by the brain, or indirectly, by enhancing lipogenesis, or by both processes. Involvement of glycerol in lipogenesis with esterification to accumulated FFA might lead to improved coupling of oxidative phosphorylation, a hypothesis that fits the finding of improved neuronal function despite further decrease in cerebral hemispheric oxygen consumption."}
{"id": "PMID:1090399", "title": "Serum zinc, iron, and copper concentrations during typhoid fever in man: effect of chloramphenicol therapy.", "content": "In volunteers experimentally infected with Salmonella typhi, serum iron and zinc concentrations became significantly depressed and there was a concomitant rise in serum copper before the onset of overt clinical illness. However, after several days of fever and the initiation of chloramphenicol therapy, serum iron and zinc concentrations significantly increased. Additional studies--in volunteers with typhoid fever treated with chloramphenicol, in a volunteer with typhoid fever receiving cefazolin and gentamicin, and in untreated rhesus monkeys infected with Salmonella typhimurium--provided evidence that the increase in serum iron concentration during the febrile phase was the result of chloramphenicol therapy, whereas the increase in serum zinc concentrations was a disease-related phenomenon. The importance of trace-metal monitoring during infectious disease and chemotherapy is discussed.", "contents": "Serum zinc, iron, and copper concentrations during typhoid fever in man: effect of chloramphenicol therapy. In volunteers experimentally infected with Salmonella typhi, serum iron and zinc concentrations became significantly depressed and there was a concomitant rise in serum copper before the onset of overt clinical illness. However, after several days of fever and the initiation of chloramphenicol therapy, serum iron and zinc concentrations significantly increased. Additional studies--in volunteers with typhoid fever treated with chloramphenicol, in a volunteer with typhoid fever receiving cefazolin and gentamicin, and in untreated rhesus monkeys infected with Salmonella typhimurium--provided evidence that the increase in serum iron concentration during the febrile phase was the result of chloramphenicol therapy, whereas the increase in serum zinc concentrations was a disease-related phenomenon. The importance of trace-metal monitoring during infectious disease and chemotherapy is discussed."}
{"id": "PMID:1090405", "title": "Deformities of the midface resulting from malunited orbital and naso-orbital fractures.", "content": "In midfacial fractures the crucial area is the upper portion of the midfacial skeleton which lodges the orbits, the interorbital space with its anatomic relationship with the anterior cranial fossa, the lacrimal apparatus, the levator muscle, and the skeletal structures of the nose. Because of the severity of the injuries in a multisystem injured patient, these fractures may be neglected or receive inadequate primary treatment. In some cases the damage is so extensive that primary treatment can only accomplish part of the task. Late reconstruction is necessary, therefore, and is the subject of this paper.", "contents": "Deformities of the midface resulting from malunited orbital and naso-orbital fractures. In midfacial fractures the crucial area is the upper portion of the midfacial skeleton which lodges the orbits, the interorbital space with its anatomic relationship with the anterior cranial fossa, the lacrimal apparatus, the levator muscle, and the skeletal structures of the nose. Because of the severity of the injuries in a multisystem injured patient, these fractures may be neglected or receive inadequate primary treatment. In some cases the damage is so extensive that primary treatment can only accomplish part of the task. Late reconstruction is necessary, therefore, and is the subject of this paper."}
{"id": "PMID:1090406", "title": "Alcohol involvement in highway crashes. A review of the epidemiologic evidence.", "content": "There is probably no other single aspect of American behavior which is so underinvestigated as driving, and yet which has such an impact on our daily lives and on our annual death rate. Evidence is reviewed which indicates that the probability of being responsible for a fatal crash increases sharply as blood alcohol concentrations increase above those achieved by heavy social drinking.", "contents": "Alcohol involvement in highway crashes. A review of the epidemiologic evidence. There is probably no other single aspect of American behavior which is so underinvestigated as driving, and yet which has such an impact on our daily lives and on our annual death rate. Evidence is reviewed which indicates that the probability of being responsible for a fatal crash increases sharply as blood alcohol concentrations increase above those achieved by heavy social drinking."}
{"id": "PMID:1090407", "title": "Facial scars resulting from vehicular accidents.", "content": "The surgeon will be better able to cope with the problem of scars as he understands the nature of wound healing and as advances are made in the knowledge of the physiology and biochemistry of tissues. Still there do remain the all-important careful handling of tissues, adequate wound repair, and adequate postoperative care of the wound and the patient.", "contents": "Facial scars resulting from vehicular accidents. The surgeon will be better able to cope with the problem of scars as he understands the nature of wound healing and as advances are made in the knowledge of the physiology and biochemistry of tissues. Still there do remain the all-important careful handling of tissues, adequate wound repair, and adequate postoperative care of the wound and the patient."}
{"id": "PMID:1090408", "title": "Midfacial fractures from vehicular accidents.", "content": "An effort is made to simplify, categorize, and outline the various forms and treatment of midfacial trauma from automotive crashes. Panfacial fractures resulting from high speed automotive accidents will continue to be a challenge to the innovative and persistent surgeon seeking the best possible results.", "contents": "Midfacial fractures from vehicular accidents. An effort is made to simplify, categorize, and outline the various forms and treatment of midfacial trauma from automotive crashes. Panfacial fractures resulting from high speed automotive accidents will continue to be a challenge to the innovative and persistent surgeon seeking the best possible results."}
{"id": "PMID:1090409", "title": "Soft tissue facial injuries related to vehicular accidents.", "content": "Most facial injuries are not dire emergencies, except in the case of airway obstruction and in unrecognized, prolonged bleeding or oozing from facial wounds. Yet one must keep in mind the psychologic impact that facial injuries may have upon the patient and his family.", "contents": "Soft tissue facial injuries related to vehicular accidents. Most facial injuries are not dire emergencies, except in the case of airway obstruction and in unrecognized, prolonged bleeding or oozing from facial wounds. Yet one must keep in mind the psychologic impact that facial injuries may have upon the patient and his family."}
{"id": "PMID:1090410", "title": "Frontal sinus and supraorbital fractures from vehicle accidents.", "content": "Fractures of the upper third of the face from vehicle accidents are relatively uncommon but can have serious associated injuries of the dura and frontal lobe when they do occur. The injury may result from the victim's face either striking the dashboard or bouncing violently off the lower broken edge of the penetrated windshield.", "contents": "Frontal sinus and supraorbital fractures from vehicle accidents. Fractures of the upper third of the face from vehicle accidents are relatively uncommon but can have serious associated injuries of the dura and frontal lobe when they do occur. The injury may result from the victim's face either striking the dashboard or bouncing violently off the lower broken edge of the penetrated windshield."}
{"id": "PMID:1090415", "title": "A double-blind study in hypertensive patients of an original new compound, indapamide.", "content": "In a double-bind crossover study in 18 patients with essential hypertension, the hypotensive activity of 5 mg. indapamide daily was compared with 40 mg. frusemide daily over a period of 4 months after an initial 15 days on placebo. The overall clinical assessment showed satisfactory blood pressure control in 72% of patients receiving indapamide compared with 57% on frusemide. The weight of patients on active therapy dropped significantly with both products, but to a greater extent with indapamide. Indapamide was well-tolerated by all 18 patients; 3 patients on frusemide developed side-effects. The results of blood chemistry investigations are discussed. Variations in potassium levels during indapamide therapy were modest and did not warrant the use of potassium supplements.", "contents": "A double-blind study in hypertensive patients of an original new compound, indapamide. In a double-bind crossover study in 18 patients with essential hypertension, the hypotensive activity of 5 mg. indapamide daily was compared with 40 mg. frusemide daily over a period of 4 months after an initial 15 days on placebo. The overall clinical assessment showed satisfactory blood pressure control in 72% of patients receiving indapamide compared with 57% on frusemide. The weight of patients on active therapy dropped significantly with both products, but to a greater extent with indapamide. Indapamide was well-tolerated by all 18 patients; 3 patients on frusemide developed side-effects. The results of blood chemistry investigations are discussed. Variations in potassium levels during indapamide therapy were modest and did not warrant the use of potassium supplements."}
{"id": "PMID:1090416", "title": "The treatment of acute anxiety states in neurotic patients with intravenous lorazepam. A placebo-controlled study.", "content": "In a double-blind trial, 20 neurotic patients with acute anxiety were treated with 3 mg. lorazepan intravenously and 17 others were treated with placebo. A total of 24 injections of lorazepam and 26 of plter compared with 9 patients on placebo (p smaller than 0.05). Sixty minutes after the injections of lorazepam, patients were free of anxiety symptoms on 70.8% occasions, compared with only 7.7% occasions following placebo (p smaller than 0.01). The effects of lorazepam on anxiety were discernible 10 minutes after the injections. Considering the number of patients, not injections, 85.0% were free of symptoms after treatment with lorazepam and only 11.8% after treatment with placebo (p smaller than 0.01). Blood pressure, pulse rate and respiratory rate were favourably influenced by the drug and not by placebo; these effects were discernible 10 minutes after the injection.", "contents": "The treatment of acute anxiety states in neurotic patients with intravenous lorazepam. A placebo-controlled study. In a double-blind trial, 20 neurotic patients with acute anxiety were treated with 3 mg. lorazepan intravenously and 17 others were treated with placebo. A total of 24 injections of lorazepam and 26 of plter compared with 9 patients on placebo (p smaller than 0.05). Sixty minutes after the injections of lorazepam, patients were free of anxiety symptoms on 70.8% occasions, compared with only 7.7% occasions following placebo (p smaller than 0.01). The effects of lorazepam on anxiety were discernible 10 minutes after the injections. Considering the number of patients, not injections, 85.0% were free of symptoms after treatment with lorazepam and only 11.8% after treatment with placebo (p smaller than 0.01). Blood pressure, pulse rate and respiratory rate were favourably influenced by the drug and not by placebo; these effects were discernible 10 minutes after the injection."}
{"id": "PMID:1090417", "title": "double-blind\"double-bind.", "content": "In a double-blind trial over a 2-week period in 34 patients with psoriases vulgaris, clobetasol propionate cream was shown to be more effective than betamethasone valerate cream. In a further 16 patients whose psoriasis had not responded to betamethasone valerate ointment, treatment with clobetasol propionate ointment was substituted. Fourteen of the 16 patients experienced a rapid improvement of their lesions.", "contents": "double-blind\"double-bind. In a double-blind trial over a 2-week period in 34 patients with psoriases vulgaris, clobetasol propionate cream was shown to be more effective than betamethasone valerate cream. In a further 16 patients whose psoriasis had not responded to betamethasone valerate ointment, treatment with clobetasol propionate ointment was substituted. Fourteen of the 16 patients experienced a rapid improvement of their lesions."}
{"id": "PMID:1090418", "title": "Antihypertensive activity of a new agent, indapamide: a double-blind study.", "content": "In a double-blind trail in 22 patients with mild to moderate essential hypertension, indapamide was compared with chlorathiazide and placebo. Dosage levels were set at 5 mg. indapamide and 500 mg. chlorothiazide daily, for 5 days out of 7, and patients were treated alternately, in random sequence, with each drug for a month over a 3-month period. Blood pressure readings and blood chemistry investigations were carried out before and after each treatment period and a careful history was kept of subjective symptoms and patients' tolerance of therapy. The results show that both active treatments produced drops in systolic and diastolic pressures, but were only statisically significant and different from placebo in the case of indapamide. Indapamide also produce much greater subjective improvement (74%) in patients with functional symptoms compared with chlorothiazide (15%). In an overall assessment, indapamide produced an excellent to good response to treatment in 57% of patients. Comparable responses for chlorotiazide and placebo were 25% and 20% respectivlely. Using patients as their own controls to compare the relative effectiveness of the three periods of treatment, indapamide was shown to be more effective than placebo in 65% of cases and more effective han chlorothiazide in 60%. Although patient tolerance of indapamide was slightly better, both drugs were well accepted and no significant changes from baseline levels were noted in any of the laboratory parameters investigated.", "contents": "Antihypertensive activity of a new agent, indapamide: a double-blind study. In a double-blind trail in 22 patients with mild to moderate essential hypertension, indapamide was compared with chlorathiazide and placebo. Dosage levels were set at 5 mg. indapamide and 500 mg. chlorothiazide daily, for 5 days out of 7, and patients were treated alternately, in random sequence, with each drug for a month over a 3-month period. Blood pressure readings and blood chemistry investigations were carried out before and after each treatment period and a careful history was kept of subjective symptoms and patients' tolerance of therapy. The results show that both active treatments produced drops in systolic and diastolic pressures, but were only statisically significant and different from placebo in the case of indapamide. Indapamide also produce much greater subjective improvement (74%) in patients with functional symptoms compared with chlorothiazide (15%). In an overall assessment, indapamide produced an excellent to good response to treatment in 57% of patients. Comparable responses for chlorotiazide and placebo were 25% and 20% respectivlely. Using patients as their own controls to compare the relative effectiveness of the three periods of treatment, indapamide was shown to be more effective than placebo in 65% of cases and more effective han chlorothiazide in 60%. Although patient tolerance of indapamide was slightly better, both drugs were well accepted and no significant changes from baseline levels were noted in any of the laboratory parameters investigated."}
{"id": "PMID:1090419", "title": "Dissociation between indices of pump performance and contractility in patients with coronary artery disease and acute myocardial infarction.", "content": "An analysis of ventricular performance comparing pump function and muscle function indices was performed in 13 patients with acute myocardial infarction, ten patients with normal coronary arteries, and 15 patients with coronary artery disease. Pump function was described by plotting left ventricular stroke work index as a function of left ventricular end diastolic pressure. This description provided a clear separation between normal patients, and surviving and nonsurviving patients with acute myocardial infarction. Values of contractile element velocity (VCE5 as an estimate of Vmax) did not separate between normals and surviving or nonsurviving patients with acute myocardial infarction. In 15 patients with acute coronary artery disease there was no correlation between values of VCE5 and the ventricular function curve. Changes in performance following the stress of ventriculography, angiotensin infusion, or isometric hand grip exercise also did not show any correlation between pump function and muscle function indices. It is concluded that pump function indices are a better indicator of ventricular performance in patients with acute myocardial infarction and coronary artery disease.", "contents": "Dissociation between indices of pump performance and contractility in patients with coronary artery disease and acute myocardial infarction. An analysis of ventricular performance comparing pump function and muscle function indices was performed in 13 patients with acute myocardial infarction, ten patients with normal coronary arteries, and 15 patients with coronary artery disease. Pump function was described by plotting left ventricular stroke work index as a function of left ventricular end diastolic pressure. This description provided a clear separation between normal patients, and surviving and nonsurviving patients with acute myocardial infarction. Values of contractile element velocity (VCE5 as an estimate of Vmax) did not separate between normals and surviving or nonsurviving patients with acute myocardial infarction. In 15 patients with acute coronary artery disease there was no correlation between values of VCE5 and the ventricular function curve. Changes in performance following the stress of ventriculography, angiotensin infusion, or isometric hand grip exercise also did not show any correlation between pump function and muscle function indices. It is concluded that pump function indices are a better indicator of ventricular performance in patients with acute myocardial infarction and coronary artery disease."}
{"id": "PMID:1090420", "title": "High level positive end expiratory pressure (PEEP) in acute respiratory insufficiency.", "content": "Twenty-eight patients developed severe, progressive acute respiratory insufficiency despite aggressive application of conventional respiratory  therapy. Application of increased PEEP (18 torr or greater) resulted in a significant decrease in QA/QT. Selection of the optimal levle of PEEP for each patient required serial determinations of QA/QT and measurement of cardiovascular response. The overall survival rate was 61 percent. Acute respiratory insufficiency was a proximate cause of death in only one patient. Four of the patients (14 percent) developed a pneumothorax following institution of high PEEP therapy. Cardiac output was not affected adversely at any level of PEEP up to 32 torr (44 cm H2O). We conclude that high levels of PEEP can be therapeutic for patients with refractory respiratory failure when combined with intermittent mandatory ventilation and careful cardiovascular monitoring. As with any therapy, the optimum dose should be tailored to each patient according to his needs and response.", "contents": "High level positive end expiratory pressure (PEEP) in acute respiratory insufficiency. Twenty-eight patients developed severe, progressive acute respiratory insufficiency despite aggressive application of conventional respiratory  therapy. Application of increased PEEP (18 torr or greater) resulted in a significant decrease in QA/QT. Selection of the optimal levle of PEEP for each patient required serial determinations of QA/QT and measurement of cardiovascular response. The overall survival rate was 61 percent. Acute respiratory insufficiency was a proximate cause of death in only one patient. Four of the patients (14 percent) developed a pneumothorax following institution of high PEEP therapy. Cardiac output was not affected adversely at any level of PEEP up to 32 torr (44 cm H2O). We conclude that high levels of PEEP can be therapeutic for patients with refractory respiratory failure when combined with intermittent mandatory ventilation and careful cardiovascular monitoring. As with any therapy, the optimum dose should be tailored to each patient according to his needs and response."}
{"id": "PMID:1090422", "title": "Chicken soup rebound and relapse of pneumonia: report of a case.", "content": "A case is reported in which a previously healthy individual, having received an inadequate course of chicken soup in treatment of mild pneumococcal pneumonia, experienced a severe relapse, refractory to all medical treatment and eventually requiring thoracotomy. The pharmacology of chicken soup is reviewed and the dangers of abrupt termination of therapy are stressed.", "contents": "Chicken soup rebound and relapse of pneumonia: report of a case. A case is reported in which a previously healthy individual, having received an inadequate course of chicken soup in treatment of mild pneumococcal pneumonia, experienced a severe relapse, refractory to all medical treatment and eventually requiring thoracotomy. The pharmacology of chicken soup is reviewed and the dangers of abrupt termination of therapy are stressed."}
{"id": "PMID:1090423", "title": "Bronchiectasis and homozygous alpha1-antitrypsin deficiency.", "content": "A 34-year-old woman with homozygous a1-antitrypsin deficiency suffered from progressive, generalized cystic bronchiectasis. Although bronchiectasis was reported in the original monograph on the enzyme inhibitor deficiency, it has received minimal attention since then. Alpha1-antitrypsin levels should be measured in patients with severe bronchiectasis.", "contents": "Bronchiectasis and homozygous alpha1-antitrypsin deficiency. A 34-year-old woman with homozygous a1-antitrypsin deficiency suffered from progressive, generalized cystic bronchiectasis. Although bronchiectasis was reported in the original monograph on the enzyme inhibitor deficiency, it has received minimal attention since then. Alpha1-antitrypsin levels should be measured in patients with severe bronchiectasis."}
{"id": "PMID:1090424", "title": "Extensive linear \"blow-out\" of the thoracic membranes trachea with innominate artery avulsion secondary to blunt chest trauma.", "content": "The successful management of extensive rupture of the entire intrathoracic membranous trachea and an avulsed innominate artery is presented. Taken separately, the two entities are in themselves rare following blunt trauma to the chest. Ventilatory and anesthetic control during repair of this injury is presented.", "contents": "Extensive linear \"blow-out\" of the thoracic membranes trachea with innominate artery avulsion secondary to blunt chest trauma. The successful management of extensive rupture of the entire intrathoracic membranous trachea and an avulsed innominate artery is presented. Taken separately, the two entities are in themselves rare following blunt trauma to the chest. Ventilatory and anesthetic control during repair of this injury is presented."}
{"id": "PMID:1090426", "title": "Vector analysis of postcardiotomy behavioral phenomena.", "content": "The classification of postcardiotomy behavioral phenomena in Figure 1 is proposed for use as a clinical instrument to analyze etiological determinants. The utilization of a vector analysis analogy inherently denies absolutism. Classifications A-P are presented as prototypes of certain ratio imbalances of the metabolic, hemodynamic, environmental, and psychic vectors. Such a system allows for change from one type to another according to the individuality of the patient and the highly specific changes in his clinical presentation. A vector analysis also allows for infinite intermediary ratio imbalances between classification types as a function of time. Thus, postcardiotomy behavioral phenomena could be viewed as the vector summation of hemodynamic, metabolic, environmental, and psychic processes at a given point in time. Elaboration of unknown determinants in this complex syndrome appears to be task for the future.", "contents": "Vector analysis of postcardiotomy behavioral phenomena. The classification of postcardiotomy behavioral phenomena in Figure 1 is proposed for use as a clinical instrument to analyze etiological determinants. The utilization of a vector analysis analogy inherently denies absolutism. Classifications A-P are presented as prototypes of certain ratio imbalances of the metabolic, hemodynamic, environmental, and psychic vectors. Such a system allows for change from one type to another according to the individuality of the patient and the highly specific changes in his clinical presentation. A vector analysis also allows for infinite intermediary ratio imbalances between classification types as a function of time. Thus, postcardiotomy behavioral phenomena could be viewed as the vector summation of hemodynamic, metabolic, environmental, and psychic processes at a given point in time. Elaboration of unknown determinants in this complex syndrome appears to be task for the future."}
{"id": "PMID:1090427", "title": "Pipotiazine palmitate: an evaluation of a new long acting intramuscular antipsychotic agent in severely ill schizophrenic patients.", "content": "Findings in this study support earlier investigations in attesting to the antipsychotic efficacy and relatively low toxicity of pipotiazine palmitate. Results with all efficacy measures utilized were consistent in indicating a high level of efficacy for this investigational compound. Pipotiazine palmitate apparently has an average duration of action that extends beyond 4 weeks in severely ill schizophrenic patients. This particular long acting IM antipsychotic preparation appears to have an even longer duration of activity than some of the other available standard long acting agents. The optimal dosage range for severely ill schizophrenic patients appears to be between 100 and 600 mg once monthly. While this type of drug (as is the case with many antipsychotic drugs) does reduce the psychotic symptomatology and improves the thought associations sufficient to enable the patient to leave the hospital, it should be re-emphasized that socioeconomic and guidance counseling services are necessary to maintain the patient in the community. The availability of this type of long acting preparation is not only economical in terms of nursing care and hospital cost but it should also increase the efficacy of psychopharmacologic treatment of schizophrenics by reducing both patient errors and staff errors in administration of medication. In addition, this IM preparation should prove to be of invaluable help in maintaining the schizophrenic patient in his community by reducing the relapse and the rehospitalization rates. It should be noted that there are schizophrenic patients who either absorb compounds from the gastrointestinal tract in a very poor manner or too rapidly metabolize the antipsychotic agents with resultant suboptimal blood levels and these subjects may be called \"drug refractory.\" This type of long acting medication is an ideal preparation for the schizophrenic patient who has these types of absorption or metabolic problems since the \"circulatory pass\" through the liver is minimal after IM medication as compared to that encountered by an orally administered agent. The clinical disadvantage of pipotiazine palmitate is the delay in onset of therapeutic activity after injection. Significant improvement is first noted after 3 to 4 days after the highest IM dosage administration. Therefore, it may be necessary to use an oral or IM preparation of a neuroleptic with a more rapid onset of activity or utilize an oral dosage of the pipotiazine salt during the first week following IM administration of pipotiazine palmitate.", "contents": "Pipotiazine palmitate: an evaluation of a new long acting intramuscular antipsychotic agent in severely ill schizophrenic patients. Findings in this study support earlier investigations in attesting to the antipsychotic efficacy and relatively low toxicity of pipotiazine palmitate. Results with all efficacy measures utilized were consistent in indicating a high level of efficacy for this investigational compound. Pipotiazine palmitate apparently has an average duration of action that extends beyond 4 weeks in severely ill schizophrenic patients. This particular long acting IM antipsychotic preparation appears to have an even longer duration of activity than some of the other available standard long acting agents. The optimal dosage range for severely ill schizophrenic patients appears to be between 100 and 600 mg once monthly. While this type of drug (as is the case with many antipsychotic drugs) does reduce the psychotic symptomatology and improves the thought associations sufficient to enable the patient to leave the hospital, it should be re-emphasized that socioeconomic and guidance counseling services are necessary to maintain the patient in the community. The availability of this type of long acting preparation is not only economical in terms of nursing care and hospital cost but it should also increase the efficacy of psychopharmacologic treatment of schizophrenics by reducing both patient errors and staff errors in administration of medication. In addition, this IM preparation should prove to be of invaluable help in maintaining the schizophrenic patient in his community by reducing the relapse and the rehospitalization rates. It should be noted that there are schizophrenic patients who either absorb compounds from the gastrointestinal tract in a very poor manner or too rapidly metabolize the antipsychotic agents with resultant suboptimal blood levels and these subjects may be called \"drug refractory.\" This type of long acting medication is an ideal preparation for the schizophrenic patient who has these types of absorption or metabolic problems since the \"circulatory pass\" through the liver is minimal after IM medication as compared to that encountered by an orally administered agent. The clinical disadvantage of pipotiazine palmitate is the delay in onset of therapeutic activity after injection. Significant improvement is first noted after 3 to 4 days after the highest IM dosage administration. Therefore, it may be necessary to use an oral or IM preparation of a neuroleptic with a more rapid onset of activity or utilize an oral dosage of the pipotiazine salt during the first week following IM administration of pipotiazine palmitate."}
{"id": "PMID:1090428", "title": "[The effect of synthetic somatostatin in normal and acromegalic males].", "content": "The synthetic linear tetradekapeptide somatostatin (growth-hormone release inhibitory hormone: GHRIH) inhibits the liberation of growth hormone in normal persons in the insulin hypoglycaemia test without influencing the rise of cortisol and prolactin, while the concentrations of LH, FSH and TSH remain unchanged. In patients with florid acromegaly there occurs during administration of GHRIH a marked fall in the raised STH level without influencing the basal level of the other anterior-pituitary hormones. As a further effect there is suppression of the insulin level. The somatostatin at present available has a very short biological half-life and in its present form is, therefore, without therapeutic importance.", "contents": "[The effect of synthetic somatostatin in normal and acromegalic males]. The synthetic linear tetradekapeptide somatostatin (growth-hormone release inhibitory hormone: GHRIH) inhibits the liberation of growth hormone in normal persons in the insulin hypoglycaemia test without influencing the rise of cortisol and prolactin, while the concentrations of LH, FSH and TSH remain unchanged. In patients with florid acromegaly there occurs during administration of GHRIH a marked fall in the raised STH level without influencing the basal level of the other anterior-pituitary hormones. As a further effect there is suppression of the insulin level. The somatostatin at present available has a very short biological half-life and in its present form is, therefore, without therapeutic importance."}
{"id": "PMID:1090429", "title": "[Antinuclear factors in patients with pancreatitis of unknown aetiology: A pointer to an immunopathological cause? (Author's transl)].", "content": "Antinuclear factors in serum were determined (in addition to other tissue antibodies) in 9 patients with acute and 22 with chronic pancreatitis. Of the 11 with pancreatitis of unknown aetiology 9 had serum antinuclear factors, most of them in high titres. Of the 20 with pancreatitis of known aetiology only 3 had antinuclear factors.", "contents": "[Antinuclear factors in patients with pancreatitis of unknown aetiology: A pointer to an immunopathological cause? (Author's transl)]. Antinuclear factors in serum were determined (in addition to other tissue antibodies) in 9 patients with acute and 22 with chronic pancreatitis. Of the 11 with pancreatitis of unknown aetiology 9 had serum antinuclear factors, most of them in high titres. Of the 20 with pancreatitis of known aetiology only 3 had antinuclear factors."}
{"id": "PMID:1090431", "title": "[Amenorrhoea after oral contraceptives].", "content": "Eleven women with amenorrhoea after taking oral contraceptives were studied.  A clomiphene stimulation test was performed in ten, with six also having an LH-RH stimulation test.  In most of them there was no reaction or a severely impaired response to both clomiphene and LH-RH, but bleeding episodes could be provoked in most women by continuing clomiphene treatment, and some of these ovulated when HCG was added to the therapeutic scheme.  A possible relationship between preexisting psychosomatic disorders and the genesis of postcontraceptive amenorrhoea is discussed.", "contents": "[Amenorrhoea after oral contraceptives]. Eleven women with amenorrhoea after taking oral contraceptives were studied.  A clomiphene stimulation test was performed in ten, with six also having an LH-RH stimulation test.  In most of them there was no reaction or a severely impaired response to both clomiphene and LH-RH, but bleeding episodes could be provoked in most women by continuing clomiphene treatment, and some of these ovulated when HCG was added to the therapeutic scheme.  A possible relationship between preexisting psychosomatic disorders and the genesis of postcontraceptive amenorrhoea is discussed."}
{"id": "PMID:1090432", "title": "Effects of gonadal steroids on the basal and LRF-induced gonadotropin secretion by cultures of rat pituitary.", "content": "The effects of 17beta-estradiol (E2), progesterone (P4), 20alpha-hydroxypregn-4-en-3-one (20alpha-OHP4), and testosterone (T) on the basal and LRF-induced secretion of LH and FSH were studies in monolayer cultures prepared from the pituitaries of adult female rats. Day 4 cultures were used and all steroids were tested at 10-8M concentration for 4 hr. E2 (2.72 ng/ml) alone caused a nonsignificant increase in basal secretion of both LH and FSH; however, the same dose of E2 significantly (p less than 0.001) inhibited the LRF-induced secretion of LH but not of FSH (74% and 88% of 10-8M LRF-treated level, respectively), Testosterone (2.88 ng/ml) alone significantly increased the basal secretion of LH (136% of control level, p less than 0.05) and augmented the effect of LRF on FSH secretion to 130% of the LRF-treated level (p less than 0.05). Contrary to its negative feedback action on the basal secretion of FSH (46% of control level, P less than 0.05), 20ALPHA-OPH4 (3.15 ng/ml) augmented the effect of LRF on LH secretion (130% of LRF-treated level, P less than 0.05). On the other hand, P4 (3.14 ng/ml) did not cause any significant inhibition in the basal and LRF-induced secretion of either LH or FSH. These data indicate that both of the LH- and FSH-LRF; however, their secretory activities are modulated differently by various steroids.", "contents": "Effects of gonadal steroids on the basal and LRF-induced gonadotropin secretion by cultures of rat pituitary. The effects of 17beta-estradiol (E2), progesterone (P4), 20alpha-hydroxypregn-4-en-3-one (20alpha-OHP4), and testosterone (T) on the basal and LRF-induced secretion of LH and FSH were studies in monolayer cultures prepared from the pituitaries of adult female rats. Day 4 cultures were used and all steroids were tested at 10-8M concentration for 4 hr. E2 (2.72 ng/ml) alone caused a nonsignificant increase in basal secretion of both LH and FSH; however, the same dose of E2 significantly (p less than 0.001) inhibited the LRF-induced secretion of LH but not of FSH (74% and 88% of 10-8M LRF-treated level, respectively), Testosterone (2.88 ng/ml) alone significantly increased the basal secretion of LH (136% of control level, p less than 0.05) and augmented the effect of LRF on FSH secretion to 130% of the LRF-treated level (p less than 0.05). Contrary to its negative feedback action on the basal secretion of FSH (46% of control level, P less than 0.05), 20ALPHA-OPH4 (3.15 ng/ml) augmented the effect of LRF on LH secretion (130% of LRF-treated level, P less than 0.05). On the other hand, P4 (3.14 ng/ml) did not cause any significant inhibition in the basal and LRF-induced secretion of either LH or FSH. These data indicate that both of the LH- and FSH-LRF; however, their secretory activities are modulated differently by various steroids."}
{"id": "PMID:1090433", "title": "Radioimmunoassay for luteinizing hormone-releasing hormone (LHRH): its application to the measurement of LHRH in ovine and human plasma.", "content": "A radioimmunoassay for LHRH, MODIFIED FROM THe assay of Nett et al. (J Clin Endocrinol Metab 36: 880, 1973) and characterized in more detail, was applied to the measurement of endogenous plasma LHRH in the peripheral circulation of ewes and women around the time of gonadotropin release. Sensitivity was 1.2 pg/tube. Within- and between-assay coefficients of variation were 10% and 25%, respectively, over the range 10-100 pg/tube. Minimal cross reaction (less than 0.1%) was observed with LHRH analogues tested, except those which had undergone single amino acid alterations in the 4 or 8 positions (62-93%) and in the 1 position (1-3%). Inhibition curves parallel to synthetic LHRH were obtained with these immunoreactive analogs, and with crude hypothalamic and pituitary extracts from 16 cycling ewes and 3 pooled hypothalamic extracts from male rats. LHRH contents of ovine hypothalami and pituitaries ranged from 1.9-10.2 (4.5 plus or minus 2.1, mean plus or minus SD) and 0-27.5 (4.2 plus or minus 7.3, mean plus or minus SD) ng LHRH, respectively, with no obvious correlation between the contents of either tissue. LHRH contents of pooled hypothalami from normal, castrated-hypophysectomized male rats were 4.5, 2.9 and 1. 3 ng/hypothalamus. Recovery of synthetic LHRH added to plasma was quantitative, provided the storage time at 4 and 20 C was minimal. Synthetic LHRH  administered to ewes by intravenous infusion or subcutaneous injection was readily detectable in peripheral plasma. The metabolic clearance rates of synthetic LHRH' in 3 sheep were 14, 11 and 14-1/day/kg, respectively. Endogenous immunoreactive material in single, unextracted plasma samples from sheep in various physiological states ranged from 0-400 pg/ml. However, the endogenous plasma immunoreactivity in these samples bone no resemblance to synthetic LHRH added to ovine plasma in that it was stable to gentle heat, and was undetectable after methanol extraction. No endogenous LHRH could be detected in methanol extracts of peripheral plasma obtained from estrous ewes or oophorectomized ewes injected iv with estradiol-17beta (40mug) and bled at 15 min intervals prior to gonadotropin release. Single daily plasma samples obtained from three normal women over their entire menstrual cycles contained no significant levels of endogenous LHRH.", "contents": "Radioimmunoassay for luteinizing hormone-releasing hormone (LHRH): its application to the measurement of LHRH in ovine and human plasma. A radioimmunoassay for LHRH, MODIFIED FROM THe assay of Nett et al. (J Clin Endocrinol Metab 36: 880, 1973) and characterized in more detail, was applied to the measurement of endogenous plasma LHRH in the peripheral circulation of ewes and women around the time of gonadotropin release. Sensitivity was 1.2 pg/tube. Within- and between-assay coefficients of variation were 10% and 25%, respectively, over the range 10-100 pg/tube. Minimal cross reaction (less than 0.1%) was observed with LHRH analogues tested, except those which had undergone single amino acid alterations in the 4 or 8 positions (62-93%) and in the 1 position (1-3%). Inhibition curves parallel to synthetic LHRH were obtained with these immunoreactive analogs, and with crude hypothalamic and pituitary extracts from 16 cycling ewes and 3 pooled hypothalamic extracts from male rats. LHRH contents of ovine hypothalami and pituitaries ranged from 1.9-10.2 (4.5 plus or minus 2.1, mean plus or minus SD) and 0-27.5 (4.2 plus or minus 7.3, mean plus or minus SD) ng LHRH, respectively, with no obvious correlation between the contents of either tissue. LHRH contents of pooled hypothalami from normal, castrated-hypophysectomized male rats were 4.5, 2.9 and 1. 3 ng/hypothalamus. Recovery of synthetic LHRH added to plasma was quantitative, provided the storage time at 4 and 20 C was minimal. Synthetic LHRH  administered to ewes by intravenous infusion or subcutaneous injection was readily detectable in peripheral plasma. The metabolic clearance rates of synthetic LHRH' in 3 sheep were 14, 11 and 14-1/day/kg, respectively. Endogenous immunoreactive material in single, unextracted plasma samples from sheep in various physiological states ranged from 0-400 pg/ml. However, the endogenous plasma immunoreactivity in these samples bone no resemblance to synthetic LHRH added to ovine plasma in that it was stable to gentle heat, and was undetectable after methanol extraction. No endogenous LHRH could be detected in methanol extracts of peripheral plasma obtained from estrous ewes or oophorectomized ewes injected iv with estradiol-17beta (40mug) and bled at 15 min intervals prior to gonadotropin release. Single daily plasma samples obtained from three normal women over their entire menstrual cycles contained no significant levels of endogenous LHRH."}
{"id": "PMID:1090434", "title": "Effects of estradiol-17beta on LH-RH/FSH-RH-induced, and spontaneous, LH release in prepubertal female pigs.", "content": "The effect of estrogen (E2) on pituitary response to LH-RH/FSH-RH (LH-RH) was examined in immature female pigs (gilts). Eight gilts (9-10 weeks old) received iv injections of 2.5, 5.0, 10.0, 20.0, and 40.0 mug LH-RH, beginning 4 days after placement of indwelling jugular cannulae. Blood samples were drawn at 5-min intervals following LH-RH injection and plasma LH was determined by RIA and expressed as ng R786-3/ml. Pituitary responses were expressed as the increment in LH levels above baseline (delta LH). Pretreatment LH levels were 0.45-1.25 ng/ml. Responses to LH-RH (delta LH plus or minus SE) ranged from 3.7 plus or minus 0.6 (at 5 min) to 8.4 plus or minus 1.4 ng/ml (at 20 min) for 2.5 and 40 mug doses, respectively. Three additional prepubertal gilts were cannulated and implanted with E2. After 3 days plasma estrogens rose from approximately 30 pg/ml to 289 plus or minus 34 pg/ml and the dose-response studies were repeated. Prestimulation LH concentration was consistently below 0.4 ng/ml and delta LH levels ranged from 1.4 plus or minus 0.5 (at 10 min) to 5.7 plus or minus 1.3 ng/ml (at 20 min) for the 2.5 and 40.0 mug doses, respectively. Analysis of the regression equations for delta LH on log LH-RH dose showed that the adjusted means for LH response were significantly lower (P less than 0.02) in E2 implanted animals. However, the regression coefficients were not different (P greater than 0.5). On the basis of these and other analyses, it is concluded that chronic E2 treatment depresses both basal LH secretion and pituitary responsiveness, but that pituitary sensitivity to LH-RH is not modified. Several nonimplanted females showed bursts of LH release subsequent to the LH-RH induced peak, but multiple peaks were never observed in E2 implanted gilts. An explanation was sought and 7 of the nonimplanted gilts were used to study spontaneous LH release by determining plasma LH at 10-min intervals for 20 h. During this period spontaneous episodes of LH release were observed in control animals with a mean frequency of 1.3 LH peaks/h. When 4 animals were given 5 mug E2 iv and then sampled for 12 h, episodic secretion was suppressed and the latency to the first spontaneous LH release was 9.6 plus or minus 0.8 h, significantly greater (P less than 0.02) than the latency to the first peak after saline injections (4.2 plus or minus 1.5 h) in the controls. It is suggested that \"multiple\" LH peaks occurring after initial LH-RH stimulation may represent the superimposition of spontaneous episodic release upon the induced response.", "contents": "Effects of estradiol-17beta on LH-RH/FSH-RH-induced, and spontaneous, LH release in prepubertal female pigs. The effect of estrogen (E2) on pituitary response to LH-RH/FSH-RH (LH-RH) was examined in immature female pigs (gilts). Eight gilts (9-10 weeks old) received iv injections of 2.5, 5.0, 10.0, 20.0, and 40.0 mug LH-RH, beginning 4 days after placement of indwelling jugular cannulae. Blood samples were drawn at 5-min intervals following LH-RH injection and plasma LH was determined by RIA and expressed as ng R786-3/ml. Pituitary responses were expressed as the increment in LH levels above baseline (delta LH). Pretreatment LH levels were 0.45-1.25 ng/ml. Responses to LH-RH (delta LH plus or minus SE) ranged from 3.7 plus or minus 0.6 (at 5 min) to 8.4 plus or minus 1.4 ng/ml (at 20 min) for 2.5 and 40 mug doses, respectively. Three additional prepubertal gilts were cannulated and implanted with E2. After 3 days plasma estrogens rose from approximately 30 pg/ml to 289 plus or minus 34 pg/ml and the dose-response studies were repeated. Prestimulation LH concentration was consistently below 0.4 ng/ml and delta LH levels ranged from 1.4 plus or minus 0.5 (at 10 min) to 5.7 plus or minus 1.3 ng/ml (at 20 min) for the 2.5 and 40.0 mug doses, respectively. Analysis of the regression equations for delta LH on log LH-RH dose showed that the adjusted means for LH response were significantly lower (P less than 0.02) in E2 implanted animals. However, the regression coefficients were not different (P greater than 0.5). On the basis of these and other analyses, it is concluded that chronic E2 treatment depresses both basal LH secretion and pituitary responsiveness, but that pituitary sensitivity to LH-RH is not modified. Several nonimplanted females showed bursts of LH release subsequent to the LH-RH induced peak, but multiple peaks were never observed in E2 implanted gilts. An explanation was sought and 7 of the nonimplanted gilts were used to study spontaneous LH release by determining plasma LH at 10-min intervals for 20 h. During this period spontaneous episodes of LH release were observed in control animals with a mean frequency of 1.3 LH peaks/h. When 4 animals were given 5 mug E2 iv and then sampled for 12 h, episodic secretion was suppressed and the latency to the first spontaneous LH release was 9.6 plus or minus 0.8 h, significantly greater (P less than 0.02) than the latency to the first peak after saline injections (4.2 plus or minus 1.5 h) in the controls. It is suggested that \"multiple\" LH peaks occurring after initial LH-RH stimulation may represent the superimposition of spontaneous episodic release upon the induced response."}
{"id": "PMID:1090435", "title": "Modulation of pituitary responsiveness to luteinizing hormone-releasing factor during the estrous cycle of the rat.", "content": "This study was performed to investigate pituitary responsiveness to synthetic gonadotropic hormone-releasing hormone (Gn-RH) administered during the 5-day estrous cycle of the rat and to examine whether the observed differences can be attributed to ovarian secretion of estradiol. Gn-RH was injected intra-arterially at 4:00 PM to rats previously anesthetized with sodium pentothal to block the LH surge on proestrus and thus minimize changes in LH secretion which occur throughout the estrous cycle. Pituitary responsiveness was defined as the difference between serum LH concentrations in samples obtained immediately before and 15 min after administration of 200 ng Gn-RH, a time when maximal circulating levels of LH were observed. Administration of Gn-RH was followed by a significant increase in circulating LH on all days of the estrous cycle on which the response was tested (diestrus 2, diestrus 3, proestrus, estrus). Pituitary responsiveness was relatively low on diestrus 2 and estrus and was increased slightly on diestrus 3. On proestrus, however, pituitary response to Gn-RH increased markedly, a phenomenon abolished by ovariectomy at 8:00 AM on diestrus 3. The large increase in pituitary responsiveness observed on proestrus was not restored in such ovariectomized rats when circulating estradiol concentrations were increased and maintained at approximately 150 pg/ml by SC insertion of Silastic capsules containing estradiol-17beta immediately following ovariectomy. Nevertheless, this estradiol treatment consistently elicited an LH surge in another group of ovariectomized rats not treated with sodium pentothal or Gn-RH. Although these observations indicate that an ovarian hormone is essential for the increase in pituitary response to Gn-RH on proestrus, the identity of this hormone remains to be established.", "contents": "Modulation of pituitary responsiveness to luteinizing hormone-releasing factor during the estrous cycle of the rat. This study was performed to investigate pituitary responsiveness to synthetic gonadotropic hormone-releasing hormone (Gn-RH) administered during the 5-day estrous cycle of the rat and to examine whether the observed differences can be attributed to ovarian secretion of estradiol. Gn-RH was injected intra-arterially at 4:00 PM to rats previously anesthetized with sodium pentothal to block the LH surge on proestrus and thus minimize changes in LH secretion which occur throughout the estrous cycle. Pituitary responsiveness was defined as the difference between serum LH concentrations in samples obtained immediately before and 15 min after administration of 200 ng Gn-RH, a time when maximal circulating levels of LH were observed. Administration of Gn-RH was followed by a significant increase in circulating LH on all days of the estrous cycle on which the response was tested (diestrus 2, diestrus 3, proestrus, estrus). Pituitary responsiveness was relatively low on diestrus 2 and estrus and was increased slightly on diestrus 3. On proestrus, however, pituitary response to Gn-RH increased markedly, a phenomenon abolished by ovariectomy at 8:00 AM on diestrus 3. The large increase in pituitary responsiveness observed on proestrus was not restored in such ovariectomized rats when circulating estradiol concentrations were increased and maintained at approximately 150 pg/ml by SC insertion of Silastic capsules containing estradiol-17beta immediately following ovariectomy. Nevertheless, this estradiol treatment consistently elicited an LH surge in another group of ovariectomized rats not treated with sodium pentothal or Gn-RH. Although these observations indicate that an ovarian hormone is essential for the increase in pituitary response to Gn-RH on proestrus, the identity of this hormone remains to be established."}
{"id": "PMID:1090436", "title": "Cortisol metabolism in the baboon during pregnancy and the postpartum period.", "content": "The metabolism of iv-administered 14C-cortisol (F) by pregnant baboons (107, 124 and 150 days gestation) was compared with that previously reported for nonpregnant animals and with that of animals examined 6-18 h after spontaneous vaginal delivery (178 plus or minus 4 days). Unconjugated, glucuronoside (beta-glucuronidase) and sulfate (H2SO4-ethyl acetate) fractions were extracted with ethyl acetate from urine containing more than 80% of injected 14C. Metabolites of interest were isolated by paper partition chromatography and purified by crystallization and derivative formation. Compared with nonpregnant animals, the following changes (P less than 0.05) were observed in pregnancy: (1) an increase in the percent urinary 14C in the unconjugated fraction and a decrease in the proportion of 14C appearing in the glucuronoside fraction; (2) an increase in excretion of metabolites more polar than the cortols; (3) a decrease in excretion of metabolites less polar than cortisone in the glucuronoside fraction; (4) an increase in unconjugated F excretion. Production rate of F (11.9 plus or minus 0.7 mg/day) estimated by isotope dilution and percent urinary 14C in tetrahydrocortisol and tetrahydrocortisone from the glucuronoside fraction were as in nonpregnant animals. With the exception of an increase in F production (22.7 plus or minus 0.8 mg/day), presumably the result of the stress of labor, F metabolism in the immediate postpartum period was strikingly similar to timals indicates that changes in the mother alone can account for the altered metabolic disposition of F in pregnancy and suggests that the fetus takes little part in metabolism of maternal circulating F.", "contents": "Cortisol metabolism in the baboon during pregnancy and the postpartum period. The metabolism of iv-administered 14C-cortisol (F) by pregnant baboons (107, 124 and 150 days gestation) was compared with that previously reported for nonpregnant animals and with that of animals examined 6-18 h after spontaneous vaginal delivery (178 plus or minus 4 days). Unconjugated, glucuronoside (beta-glucuronidase) and sulfate (H2SO4-ethyl acetate) fractions were extracted with ethyl acetate from urine containing more than 80% of injected 14C. Metabolites of interest were isolated by paper partition chromatography and purified by crystallization and derivative formation. Compared with nonpregnant animals, the following changes (P less than 0.05) were observed in pregnancy: (1) an increase in the percent urinary 14C in the unconjugated fraction and a decrease in the proportion of 14C appearing in the glucuronoside fraction; (2) an increase in excretion of metabolites more polar than the cortols; (3) a decrease in excretion of metabolites less polar than cortisone in the glucuronoside fraction; (4) an increase in unconjugated F excretion. Production rate of F (11.9 plus or minus 0.7 mg/day) estimated by isotope dilution and percent urinary 14C in tetrahydrocortisol and tetrahydrocortisone from the glucuronoside fraction were as in nonpregnant animals. With the exception of an increase in F production (22.7 plus or minus 0.8 mg/day), presumably the result of the stress of labor, F metabolism in the immediate postpartum period was strikingly similar to timals indicates that changes in the mother alone can account for the altered metabolic disposition of F in pregnancy and suggests that the fetus takes little part in metabolism of maternal circulating F."}
{"id": "PMID:1090437", "title": "Ovarian modulation of immunoreactive gonadotropins-releasing hormone (Gn-RH) in the rat brain: evidence for a differential effect on the anterior and mid-hypothalamus.", "content": "Gonadotropins-releasing hormone (Gn-RH) in selected regions of the female rat brain was measured by radioimmunoassay. Detectable immunoreactive Gn-RH was found in the anterior hypothalamic-septal region and in the mid-hypothalamic (arcuate-median eminence) region. Gn-RH was several times higher in the middle region than in the anterior region. Gn-RH was undetectable in the posterior hypothalamic region, frontal cerebral cortex and pineal glands, as well as in random blood samples, and low to undetectable in anterior pituitary glands. Gn-RH activity varied during the estrous cycle and after castration. In the mid-hypothalamic region, Gn-RH content was lowest throughout diestrus and in late morning and early afternoon of proestrus, and highest early in the morning of proestrus and during estrus. A significant decrease at mid-day was only found on the day of proestrus, a few hours prior to the critical period for LH release. In the anterior hypothalamic region, low Gn-RH activity was found from 1200 h of estrus to 1200 h of diestrus-2. A comparatively higher activity was seen at 1700 h of diestrus-2 and also from 1400 h of proestrus to 0800 h of estrus. Twenty-one days after ovariectomy, Gn-RH in the mid-hypothalamic region was significantly lower than the lowest values seen during the estrous cycle, while Gn-RH in the anterior hypothalamic region remained between low and high values seen during the cycle, being significantly higher than the low values. The changes observed during the estrous cycle and after castration suggest that gonadal steroids play a direct role in the control of hypothalamic Gn-RH. These data also demonstrate that Gn-RH varies in a different way in the anterior and mid-hypothalamic regions.", "contents": "Ovarian modulation of immunoreactive gonadotropins-releasing hormone (Gn-RH) in the rat brain: evidence for a differential effect on the anterior and mid-hypothalamus. Gonadotropins-releasing hormone (Gn-RH) in selected regions of the female rat brain was measured by radioimmunoassay. Detectable immunoreactive Gn-RH was found in the anterior hypothalamic-septal region and in the mid-hypothalamic (arcuate-median eminence) region. Gn-RH was several times higher in the middle region than in the anterior region. Gn-RH was undetectable in the posterior hypothalamic region, frontal cerebral cortex and pineal glands, as well as in random blood samples, and low to undetectable in anterior pituitary glands. Gn-RH activity varied during the estrous cycle and after castration. In the mid-hypothalamic region, Gn-RH content was lowest throughout diestrus and in late morning and early afternoon of proestrus, and highest early in the morning of proestrus and during estrus. A significant decrease at mid-day was only found on the day of proestrus, a few hours prior to the critical period for LH release. In the anterior hypothalamic region, low Gn-RH activity was found from 1200 h of estrus to 1200 h of diestrus-2. A comparatively higher activity was seen at 1700 h of diestrus-2 and also from 1400 h of proestrus to 0800 h of estrus. Twenty-one days after ovariectomy, Gn-RH in the mid-hypothalamic region was significantly lower than the lowest values seen during the estrous cycle, while Gn-RH in the anterior hypothalamic region remained between low and high values seen during the cycle, being significantly higher than the low values. The changes observed during the estrous cycle and after castration suggest that gonadal steroids play a direct role in the control of hypothalamic Gn-RH. These data also demonstrate that Gn-RH varies in a different way in the anterior and mid-hypothalamic regions."}
{"id": "PMID:1090438", "title": "Dual action of antidepressant drugs (MAO inhibitors) on insulin release.", "content": "Experiments have been carried out to investigate the role of monoamine oxidase (MAO) in the mechanism of insulin release. Isolated islets and pieces of rat pancreas were incubated in media of high glucose content in the presence of various concentrations of MAO inhibitors. At the end of the incubation, the islet MAO activity and the concentration of insulin released into the medium was measured. The results have shown that: 1. Glucose-mediated insulin release was potentiated by low concentrations of MAO inhibitors, the highest effect being observed at 10 muM. 2. Islet MAO activity was completely abolished in the presence of 10 muM of MAO inhibitors, while at lower concentrations, partial inhibition of enzyme could be achieved. A rise in the concentration of MAO inhibitors was accompanied by a decline in their potentiating effect on insulin secretion. No potentiation was observed at 1 mM concentration, and total inhibition of glucose-mediated insulin release was obtained in the presence of 5-10 mM of MA9 inhibitors. The potentiation of insulin release caused by two of the MAO inhibitors studied was abolished by addition of 1 muM epinephrine into the incubation medium. The basal rate of insulin release was insensitive to low concentrations of MAO inhibitors, but an inhibitory effect was obtained when concentrations were raised to 10 mM. In a comparative study, it was found that MAO activity was greater in liver than in islet tissue, while islets contained three times the activity of exocrine pancreas, consistent with previous findings in islet-cell tumors. The data presented here clearly show that MAO inhibitors are capable of both potentiating and inhibiting insulin release in vitro, depending on their concentrations. It is concluded that the stimulation of glucose-mediated insulin secretion may be related to the MAO inhibitory effects of the drugs, while the inhibition observed at concentrations greater than 1 times 10-3M is due to some other unidentified mechanism.", "contents": "Dual action of antidepressant drugs (MAO inhibitors) on insulin release. Experiments have been carried out to investigate the role of monoamine oxidase (MAO) in the mechanism of insulin release. Isolated islets and pieces of rat pancreas were incubated in media of high glucose content in the presence of various concentrations of MAO inhibitors. At the end of the incubation, the islet MAO activity and the concentration of insulin released into the medium was measured. The results have shown that: 1. Glucose-mediated insulin release was potentiated by low concentrations of MAO inhibitors, the highest effect being observed at 10 muM. 2. Islet MAO activity was completely abolished in the presence of 10 muM of MAO inhibitors, while at lower concentrations, partial inhibition of enzyme could be achieved. A rise in the concentration of MAO inhibitors was accompanied by a decline in their potentiating effect on insulin secretion. No potentiation was observed at 1 mM concentration, and total inhibition of glucose-mediated insulin release was obtained in the presence of 5-10 mM of MA9 inhibitors. The potentiation of insulin release caused by two of the MAO inhibitors studied was abolished by addition of 1 muM epinephrine into the incubation medium. The basal rate of insulin release was insensitive to low concentrations of MAO inhibitors, but an inhibitory effect was obtained when concentrations were raised to 10 mM. In a comparative study, it was found that MAO activity was greater in liver than in islet tissue, while islets contained three times the activity of exocrine pancreas, consistent with previous findings in islet-cell tumors. The data presented here clearly show that MAO inhibitors are capable of both potentiating and inhibiting insulin release in vitro, depending on their concentrations. It is concluded that the stimulation of glucose-mediated insulin secretion may be related to the MAO inhibitory effects of the drugs, while the inhibition observed at concentrations greater than 1 times 10-3M is due to some other unidentified mechanism."}
{"id": "PMID:1090448", "title": "[Pyrazolic sulfanilamides. XIV. Hydroxyderivatives of 1-phenyl-5-sulfanilamidopyrazole and of 1-phenyl-3-methyl-5-sulfanilamidopyrazole].", "content": "A report is given of the variations in bacteriostatic activity on introduction of a hydrophilic group, the hydroxyl group (-OH), at positions 2',3' and 4' of the phenyl group linked to the heterocyclic nitrogen os 1-phenyl-5-sulfanilamidopyrazole (I: R = -H) and of 1-phenyl-3-methyl-5-sulfanilamidopyrazole (II: R = -H). The substances prepared for this purpose: 1-(hydroxyphenyl)-5-sulfanilamidopyrazoles (Ia)(Ib))(Ic)(-OH at 2', 3',4') and 1-(hydroxyphenyl)-3-methyl-5-sulfanilamidopyrazoles (IIa)(IIb)(IIc)(-OH at 2',3'4') in vitro tests of bacteriostatic activity against strains of S. aureus and E. coli gave the following results: See journal for results.", "contents": "[Pyrazolic sulfanilamides. XIV. Hydroxyderivatives of 1-phenyl-5-sulfanilamidopyrazole and of 1-phenyl-3-methyl-5-sulfanilamidopyrazole]. A report is given of the variations in bacteriostatic activity on introduction of a hydrophilic group, the hydroxyl group (-OH), at positions 2',3' and 4' of the phenyl group linked to the heterocyclic nitrogen os 1-phenyl-5-sulfanilamidopyrazole (I: R = -H) and of 1-phenyl-3-methyl-5-sulfanilamidopyrazole (II: R = -H). The substances prepared for this purpose: 1-(hydroxyphenyl)-5-sulfanilamidopyrazoles (Ia)(Ib))(Ic)(-OH at 2', 3',4') and 1-(hydroxyphenyl)-3-methyl-5-sulfanilamidopyrazoles (IIa)(IIb)(IIc)(-OH at 2',3'4') in vitro tests of bacteriostatic activity against strains of S. aureus and E. coli gave the following results: See journal for results."}
{"id": "PMID:1090450", "title": "Ontogeny of mouse T-lymphocyte function.", "content": "The development of lymphocytes within the fetal and neonatal BALB/c mouse thymus is reviewed with particular emphasis on the maturity of immunologic functions. Fetal thymocytes respond by vigorous proliferation to stimulation by allogeneic lymphoid cells or by phytohemagglutinin. Such reactivity is much diminished in neonatal thymus or thymic-derived (T) cells in neonatal spleen. Splenic T cells seem to mature more slowly than immunoglobulin-bearing B lymphocytes in the neonatal spleen, but the finding is confounded by the presence of large numbers of \"suppressor\" T cells in the neonatal spleen. For example, the in vitro antibody response to the T-independent antigen dinitrophenyl-lysine-Ficoll is optimal by 2 or 3 weeks of age, but the in vitro response to T-dependent sheep erythrocytes does not reach adult levels until 6 weeks of age, suggesting a deficiency in T \"helper cells.\" The response of neonatal spleen cells to sheep erythrocytes cannot be reconstituted by adult T cells however, unless neonatal splenic T cells are first depleted by anti-Thy 1 serum and complement. The target of this T suppressor cell seems to be only B cells, and not other T cells. The overall sequence of T lymphocyte maturation in the mouse seems to start with large numbers of reactive T cells as well as some functionally active helper or effector T cells in early neonatal life, and finally to achieve a stable equilibrium between T cell subpopulations between 5 and 6 weeks of age.", "contents": "Ontogeny of mouse T-lymphocyte function. The development of lymphocytes within the fetal and neonatal BALB/c mouse thymus is reviewed with particular emphasis on the maturity of immunologic functions. Fetal thymocytes respond by vigorous proliferation to stimulation by allogeneic lymphoid cells or by phytohemagglutinin. Such reactivity is much diminished in neonatal thymus or thymic-derived (T) cells in neonatal spleen. Splenic T cells seem to mature more slowly than immunoglobulin-bearing B lymphocytes in the neonatal spleen, but the finding is confounded by the presence of large numbers of \"suppressor\" T cells in the neonatal spleen. For example, the in vitro antibody response to the T-independent antigen dinitrophenyl-lysine-Ficoll is optimal by 2 or 3 weeks of age, but the in vitro response to T-dependent sheep erythrocytes does not reach adult levels until 6 weeks of age, suggesting a deficiency in T \"helper cells.\" The response of neonatal spleen cells to sheep erythrocytes cannot be reconstituted by adult T cells however, unless neonatal splenic T cells are first depleted by anti-Thy 1 serum and complement. The target of this T suppressor cell seems to be only B cells, and not other T cells. The overall sequence of T lymphocyte maturation in the mouse seems to start with large numbers of reactive T cells as well as some functionally active helper or effector T cells in early neonatal life, and finally to achieve a stable equilibrium between T cell subpopulations between 5 and 6 weeks of age."}
{"id": "PMID:1090452", "title": "Development of B lymphocytes-1,2.", "content": "A rough outline of the development pathway of stem cells to B lymphocytes in mammals is beginning to emerge. The stem cell is most probably either identical to or closely related to the spleen colony-forming unit or hemopoietic stem cell. The stem cell appears not to be specificity-restricted and to lack Ig surface receptors. Whether part of B-cell development in mammals takes place in a specialized lymphoid environment, analogous to the bursa of Fabricius in birds, is still unresolved. The bone marrow does not form such an environment. The bone marrow and spleen, but not the lymph nodes, of mice contain a nondividing B-cell precursor population at an intermediate stage of development between the stem cell and B cell. This population has Ig surface receptors and is specificity-restricted. It cannot be initiated into differentiating into antibody-producing cells. However, under appropriate conditions it can give rise to functional B cells within 3 days. Newly-formed B cells (designated B1) are physically different from mature B cells (designated B2) and appear to be a dividing population of cells differentiating from the B-cell precursor to B2 nondividing cell populations.", "contents": "Development of B lymphocytes-1,2. A rough outline of the development pathway of stem cells to B lymphocytes in mammals is beginning to emerge. The stem cell is most probably either identical to or closely related to the spleen colony-forming unit or hemopoietic stem cell. The stem cell appears not to be specificity-restricted and to lack Ig surface receptors. Whether part of B-cell development in mammals takes place in a specialized lymphoid environment, analogous to the bursa of Fabricius in birds, is still unresolved. The bone marrow does not form such an environment. The bone marrow and spleen, but not the lymph nodes, of mice contain a nondividing B-cell precursor population at an intermediate stage of development between the stem cell and B cell. This population has Ig surface receptors and is specificity-restricted. It cannot be initiated into differentiating into antibody-producing cells. However, under appropriate conditions it can give rise to functional B cells within 3 days. Newly-formed B cells (designated B1) are physically different from mature B cells (designated B2) and appear to be a dividing population of cells differentiating from the B-cell precursor to B2 nondividing cell populations."}
{"id": "PMID:1090453", "title": "Pre- and postnatal enzyme capacity for drug metabolite production.", "content": "Most lipid-soluble foreign compounds including drugs, insecticides and many environmental pollutants are metabolized in animals by cytochrome P-450 enzymes in the endoplasmic reticulum of liver. These enzymes are virtually absent in fetuses of laboratory animals, but their activities increase to adult levels within 3-8 weeks after birth. In human fetuses, the enzymes appear during the first half of pregnancy, and their activities during gestation reach about one third of those found in adults. The species differences in fetal activities apparently parallel the differences in the development of liver endoplasmic reticulum. In laboratory animals, the rough-surfaced reticulum does not develop until 4 days before birth adn the smooth-surfaced retuculum develops only after birth. In man, however, the rough-surfaced form appears at about 7 to 9 weeks of gestation, whereas the smooth-surfaced form appears at about the 3rd month of pregnancy. Despite the early development of these enzymes in humans, they probably play only a minor role in limiting the accumulation of most foreign compounds in human fetuses. Nevertheless, they may play an important role in drug-induced toxicities, particularly those that are mediated through the formation of chemically reactive metabolites.", "contents": "Pre- and postnatal enzyme capacity for drug metabolite production. Most lipid-soluble foreign compounds including drugs, insecticides and many environmental pollutants are metabolized in animals by cytochrome P-450 enzymes in the endoplasmic reticulum of liver. These enzymes are virtually absent in fetuses of laboratory animals, but their activities increase to adult levels within 3-8 weeks after birth. In human fetuses, the enzymes appear during the first half of pregnancy, and their activities during gestation reach about one third of those found in adults. The species differences in fetal activities apparently parallel the differences in the development of liver endoplasmic reticulum. In laboratory animals, the rough-surfaced reticulum does not develop until 4 days before birth adn the smooth-surfaced retuculum develops only after birth. In man, however, the rough-surfaced form appears at about 7 to 9 weeks of gestation, whereas the smooth-surfaced form appears at about the 3rd month of pregnancy. Despite the early development of these enzymes in humans, they probably play only a minor role in limiting the accumulation of most foreign compounds in human fetuses. Nevertheless, they may play an important role in drug-induced toxicities, particularly those that are mediated through the formation of chemically reactive metabolites."}
{"id": "PMID:1090454", "title": "Indirect consequences of low-level use of antimicrobial agents in animal feeds.", "content": "Antibiotics may be classified as \"therapeutic\" or \"feed\" depending on their use for treatment of disease or for promotion of growth in animals. The mechanism of growth promotion is largely unknown, but it has been shown that the specific feed additive causes defined lesions in the cell wall of Escherichia coli. This effect may sensitize the bacteria to the action of therapeutic drugs. Damage to the bacterial cell wall may also cause the bacteria to be more susceptible to the body defense mechanisms. One form of stress may be defined in terms of a disturbance of intestinal flora brought on by relocation, group mixing, change of diet, or travel. Prolonged feeding of antimicrobial agents to animals has not been shown to be responsible for the current high level of R factors in the intestinal tract of man.", "contents": "Indirect consequences of low-level use of antimicrobial agents in animal feeds. Antibiotics may be classified as \"therapeutic\" or \"feed\" depending on their use for treatment of disease or for promotion of growth in animals. The mechanism of growth promotion is largely unknown, but it has been shown that the specific feed additive causes defined lesions in the cell wall of Escherichia coli. This effect may sensitize the bacteria to the action of therapeutic drugs. Damage to the bacterial cell wall may also cause the bacteria to be more susceptible to the body defense mechanisms. One form of stress may be defined in terms of a disturbance of intestinal flora brought on by relocation, group mixing, change of diet, or travel. Prolonged feeding of antimicrobial agents to animals has not been shown to be responsible for the current high level of R factors in the intestinal tract of man."}
{"id": "PMID:1090455", "title": "Induction of timed ovulation with synthetic luteinizing hormone-releasing hormone in women undergoing insemination therapy. I. Effect of a single parenteral administration at midcycle.", "content": "Synthetic LH-RH was administered by various routes to 19 patients during 37 treatment cycles in an attempt to trigger ovulation in association with insemination therapy. Thirty-five cycles were ovulatory; four pregnancies occurred, but two of them ended in spontaneous abortion. In 20 cycles, the hyperthermia response occurred one to three days after LH-RH administration, suggesting that ovulation occurred as a direct consequence of administered LH-RH. Low levels and delayed peak secretion of progesterone were found in two patients who had serial progesterone determinations during the luteal phase after LH-RH administration. Ovulation timing with LH-RH appears feasible, but efforts to ensure the availability of a mature follicle are required.", "contents": "Induction of timed ovulation with synthetic luteinizing hormone-releasing hormone in women undergoing insemination therapy. I. Effect of a single parenteral administration at midcycle. Synthetic LH-RH was administered by various routes to 19 patients during 37 treatment cycles in an attempt to trigger ovulation in association with insemination therapy. Thirty-five cycles were ovulatory; four pregnancies occurred, but two of them ended in spontaneous abortion. In 20 cycles, the hyperthermia response occurred one to three days after LH-RH administration, suggesting that ovulation occurred as a direct consequence of administered LH-RH. Low levels and delayed peak secretion of progesterone were found in two patients who had serial progesterone determinations during the luteal phase after LH-RH administration. Ovulation timing with LH-RH appears feasible, but efforts to ensure the availability of a mature follicle are required."}
{"id": "PMID:1090456", "title": "Use of synthetic luteinizing hormone-releasing hormone in treatment of oligospermic men: a preliminary report.", "content": "Synthetic luteinizing hormone-releasing hormone (LH-RH) was administered to four normogonadotropic, oligospermic men (24 to 39 years of age) who had no endocrinologic, urologic, or associated vascular disease, to assess its possible therapeutic value in male infertility. Previous testicular biopsies of these subjects indicated alteration of spermiogenesis only. LH-RH (mean dose, 500 mug/day) was administered intramuscularly for 100 to 135 days. Each patient had at least two sperm count before starting therapy and had one every 20 to 30 days during and for two to five months after treatment. The sperm count, semen volume, sperm motility and morphology, and seminal plasma concentrations of fructose and citric acid were studied in each semen sample. In three of the four patients, urinary LH and FSH excretion and plasma testosterone levels were also measured. The sperm count increased clearly in two subjects 30 to 80 days after therapy started; the response was small in the third subject and negative in the fourth. The remaining parameters followed variable courses. Libido increased in all subjects. In the post-treatment period, the two patients who had shown the best response during treatment experienced a new and abrupt increase in the sperm count which remained well above initial values at the end of follow-up. LH-RH appears to be of value in the treatment of certain types of oligospermia, but several issues remain unsettled.", "contents": "Use of synthetic luteinizing hormone-releasing hormone in treatment of oligospermic men: a preliminary report. Synthetic luteinizing hormone-releasing hormone (LH-RH) was administered to four normogonadotropic, oligospermic men (24 to 39 years of age) who had no endocrinologic, urologic, or associated vascular disease, to assess its possible therapeutic value in male infertility. Previous testicular biopsies of these subjects indicated alteration of spermiogenesis only. LH-RH (mean dose, 500 mug/day) was administered intramuscularly for 100 to 135 days. Each patient had at least two sperm count before starting therapy and had one every 20 to 30 days during and for two to five months after treatment. The sperm count, semen volume, sperm motility and morphology, and seminal plasma concentrations of fructose and citric acid were studied in each semen sample. In three of the four patients, urinary LH and FSH excretion and plasma testosterone levels were also measured. The sperm count increased clearly in two subjects 30 to 80 days after therapy started; the response was small in the third subject and negative in the fourth. The remaining parameters followed variable courses. Libido increased in all subjects. In the post-treatment period, the two patients who had shown the best response during treatment experienced a new and abrupt increase in the sperm count which remained well above initial values at the end of follow-up. LH-RH appears to be of value in the treatment of certain types of oligospermia, but several issues remain unsettled."}
{"id": "PMID:1090457", "title": "Unusual arrangement of agranular endoplasmic reticulum in the interstitial cells of the cat ovary.", "content": "An unusual arrangement of agranular endoplasmic reticulum in the interstitial cells of the cat ovary is described. The structural unit of this arrangement in cross section consists of five membranous sacs (800 to 900 A long and 250 to 350 A wide) radially coverging toward a microtubule. The sacs are separated from each other by three angles of 60 degrees and two of 90 degrees. In longitudinal section, the sacs appear to be long parallel cisternae. The geometric, radial arrangement seems to arise from a gradual incurvation and convergence of parallel units in these membranes. The microtubules may act as guides for the polarization and resulting unusual arrangement of the membranes. The purpose of such polarization may be related to a change from protein to steriod synthesis in these cells.", "contents": "Unusual arrangement of agranular endoplasmic reticulum in the interstitial cells of the cat ovary. An unusual arrangement of agranular endoplasmic reticulum in the interstitial cells of the cat ovary is described. The structural unit of this arrangement in cross section consists of five membranous sacs (800 to 900 A long and 250 to 350 A wide) radially coverging toward a microtubule. The sacs are separated from each other by three angles of 60 degrees and two of 90 degrees. In longitudinal section, the sacs appear to be long parallel cisternae. The geometric, radial arrangement seems to arise from a gradual incurvation and convergence of parallel units in these membranes. The microtubules may act as guides for the polarization and resulting unusual arrangement of the membranes. The purpose of such polarization may be related to a change from protein to steriod synthesis in these cells."}
{"id": "PMID:1090459", "title": "History of resins in dentistry.", "content": "The period of 175 years from 1800 to 1975 represents one of significant advancement in prosthetic and restorative dental service. The transition from the time of ill-fitting dentures, fashioned from naturally occurring materials, to the application of synthetic resins for a long list of dental and surgical purposes described in this symposium represents a typical example of technical and professional advancement that has taken place throughout the world society during this same period. As noted in the beginning of this report, the advancement in dentistry has been possible through the cooperative efforts of contemporary scientists in many related fields. If denistry retains this good working relationship, as it is expected to do, then the advancements within the next two generations can significantly change and improve the practice of dentistry from what is presently known, by the application of additional new and modified dental resins.", "contents": "History of resins in dentistry. The period of 175 years from 1800 to 1975 represents one of significant advancement in prosthetic and restorative dental service. The transition from the time of ill-fitting dentures, fashioned from naturally occurring materials, to the application of synthetic resins for a long list of dental and surgical purposes described in this symposium represents a typical example of technical and professional advancement that has taken place throughout the world society during this same period. As noted in the beginning of this report, the advancement in dentistry has been possible through the cooperative efforts of contemporary scientists in many related fields. If denistry retains this good working relationship, as it is expected to do, then the advancements within the next two generations can significantly change and improve the practice of dentistry from what is presently known, by the application of additional new and modified dental resins."}
{"id": "PMID:1090461", "title": "Construction of denture bases from pour resins.", "content": "Satisfactory dentures can be produced from pour resins at a considerable saving of processing time. The problems of porosity, voids, and heavy anterior tooth contact resulting from shrinkage of the resin during curing present difficulties which must be understood to be compensated for. The use of alginate and a recently introduced modified gypsum product as investing media, as well as centrifugation of the pour resin into the flask, offer no significant advantages over the originally introduced procedure. Further critical research will conceivably improve the pour resins and the corresponding processing techniques to the point that they can challenge heat-curing resins for the largest percentage of denture bases.", "contents": "Construction of denture bases from pour resins. Satisfactory dentures can be produced from pour resins at a considerable saving of processing time. The problems of porosity, voids, and heavy anterior tooth contact resulting from shrinkage of the resin during curing present difficulties which must be understood to be compensated for. The use of alginate and a recently introduced modified gypsum product as investing media, as well as centrifugation of the pour resin into the flask, offer no significant advantages over the originally introduced procedure. Further critical research will conceivably improve the pour resins and the corresponding processing techniques to the point that they can challenge heat-curing resins for the largest percentage of denture bases."}
{"id": "PMID:1090464", "title": "The acrylic jacket crown.", "content": "An attempt has been made to cover briefly the many applications of the acrylic jacket crown. It is readily understandable that this type of restoration has many shortcomings but at the same time it has many useful and important applications in dentistry when properly employed. It is hoped that the specialist and generalist alike will have found some new and useful applications of the acrylic jacket crown.", "contents": "The acrylic jacket crown. An attempt has been made to cover briefly the many applications of the acrylic jacket crown. It is readily understandable that this type of restoration has many shortcomings but at the same time it has many useful and important applications in dentistry when properly employed. It is hoped that the specialist and generalist alike will have found some new and useful applications of the acrylic jacket crown."}
{"id": "PMID:1090474", "title": "Stimulation of glucagon secretion by ethanol-induced hypoglycemia in man.", "content": "In fasted man, ethanol lowers plasma glucose by inhibiting gluconeogenesis with concomitant suppression of insulin release. Since glucose regulation of glucagon (IRG) secretion may be insulin dependent, we have evaluated IRG secretion in this setting of hypoglycemia and insulin deficiency. Mean IRG levels in six men fasted for fifty-six hours rose gradually from a basal level of 57 pg/ml. to 101 pg/ml. During the subsequent four-hour ethanol infusion, mean glucose concentration fell only 18 mg. per 100 ml. (26 per cent of pre-infusion values) yet IRG tripled to 265 pg/ml. Insulin (IRI) fell to unmeasurably low values. Alcohol given after only eight hours of fasting has no effect on plasma levels of glucose, IRG and IRI. These results suggest that the small decrease in extracellular glucose combined with relative insulin deficiency may cause inordinate intra-alpha cell glucopenia and result in exaggerated glucagon release.", "contents": "Stimulation of glucagon secretion by ethanol-induced hypoglycemia in man. In fasted man, ethanol lowers plasma glucose by inhibiting gluconeogenesis with concomitant suppression of insulin release. Since glucose regulation of glucagon (IRG) secretion may be insulin dependent, we have evaluated IRG secretion in this setting of hypoglycemia and insulin deficiency. Mean IRG levels in six men fasted for fifty-six hours rose gradually from a basal level of 57 pg/ml. to 101 pg/ml. During the subsequent four-hour ethanol infusion, mean glucose concentration fell only 18 mg. per 100 ml. (26 per cent of pre-infusion values) yet IRG tripled to 265 pg/ml. Insulin (IRI) fell to unmeasurably low values. Alcohol given after only eight hours of fasting has no effect on plasma levels of glucose, IRG and IRI. These results suggest that the small decrease in extracellular glucose combined with relative insulin deficiency may cause inordinate intra-alpha cell glucopenia and result in exaggerated glucagon release."}
{"id": "PMID:1090476", "title": "Kinetics of taurocholate uptake by the perfused rat liver.", "content": "The uptake of taurocholate (TC) by the perfused rat liver was studied by the multiple indicator dilution technique using single injections of 14-C-labeled TC and 99m-Tc-labeled albumin. For determination of the intra- and extravascular space, 51-Cr-labeled erythrocytes and albumin were used. TC was found to be distributed into an extravascular space equivalent to that of albumin. Analysis of the dilution curves, according to Goresky (Am J Physiol 207:13-26, 1964), revealed that the initial TC uptake rate increased with increasingly TC dose in a nonlinear fashion, exhibiting saturation kinetics which obeyed the Michaelis-Menten equation. The initial maximal uptake rats (Vmax) was found to be 32.5 nmoles per s and g of liver, a value exceeding the maximal steady state capacity (Tm) for excretion of TC into the bile. The half-saturation constant (Km) was 90.6 nmoles per g of liver, indicating that this system is operating far from saturation at physiological levels of bile acids in portal blood.", "contents": "Kinetics of taurocholate uptake by the perfused rat liver. The uptake of taurocholate (TC) by the perfused rat liver was studied by the multiple indicator dilution technique using single injections of 14-C-labeled TC and 99m-Tc-labeled albumin. For determination of the intra- and extravascular space, 51-Cr-labeled erythrocytes and albumin were used. TC was found to be distributed into an extravascular space equivalent to that of albumin. Analysis of the dilution curves, according to Goresky (Am J Physiol 207:13-26, 1964), revealed that the initial TC uptake rate increased with increasingly TC dose in a nonlinear fashion, exhibiting saturation kinetics which obeyed the Michaelis-Menten equation. The initial maximal uptake rats (Vmax) was found to be 32.5 nmoles per s and g of liver, a value exceeding the maximal steady state capacity (Tm) for excretion of TC into the bile. The half-saturation constant (Km) was 90.6 nmoles per g of liver, indicating that this system is operating far from saturation at physiological levels of bile acids in portal blood."}
{"id": "PMID:1090478", "title": "Gastrin cell hyperplasia in rats with chronic antral stimulation.", "content": "Rats surgically prepared with the antrum transposed onto the colon were compared with suitable control rats, to investigate whether chronic antral stimulation modifies gastrin cell and other endocrine cell populations. Gastrin cell and argyrophil cell density per unit area were studied in antrum using a quantitative method after staining by immunofluorescence and Grimelius argyrophily, respectively. Both gastrin cells and argyrophil cells increased significantly in density per unit area after antrocolic transposition (P smaller than 0.001). The augmentation of gastrin cells per glandular tube (78%) was also significant (P smaller than 0.001). Electron-microscopic observations confirmed these results. On the other hand, in each group, the topographic distribution of the two categories of cells was different, and the number of gastrin cells was statistically greater than that of the argyrophil cells. Thus, it appeared evident that gastrin cells were not argyrophilic. Several hypotheses to explain the mechanism of this hyperplasia of endocrine cells are discussed. It is concluded that the increase in the gastrin cell density could be one possible explanation for the hypergastrinemia observed in the antrocolic transposition model.", "contents": "Gastrin cell hyperplasia in rats with chronic antral stimulation. Rats surgically prepared with the antrum transposed onto the colon were compared with suitable control rats, to investigate whether chronic antral stimulation modifies gastrin cell and other endocrine cell populations. Gastrin cell and argyrophil cell density per unit area were studied in antrum using a quantitative method after staining by immunofluorescence and Grimelius argyrophily, respectively. Both gastrin cells and argyrophil cells increased significantly in density per unit area after antrocolic transposition (P smaller than 0.001). The augmentation of gastrin cells per glandular tube (78%) was also significant (P smaller than 0.001). Electron-microscopic observations confirmed these results. On the other hand, in each group, the topographic distribution of the two categories of cells was different, and the number of gastrin cells was statistically greater than that of the argyrophil cells. Thus, it appeared evident that gastrin cells were not argyrophilic. Several hypotheses to explain the mechanism of this hyperplasia of endocrine cells are discussed. It is concluded that the increase in the gastrin cell density could be one possible explanation for the hypergastrinemia observed in the antrocolic transposition model."}
{"id": "PMID:1090479", "title": "Computer-aided diagnosis of lower gastrointestinal tract disorders.", "content": "This paper reports a survey of human and computer-aided diagnosis in a prospective consecutive series of 301 patients admitted to the hospital with lower gastrointestinal tract disease. At initial outpatient contact (at which time endoscopy was customarily performed), the clinicians' diagnostic accuracy was 64.5%. After biopsy, radiology, and other investigative procedures, the clinicians' preoperative diagnostic accuracy rose t0 82.7%. When data from the house surgeon's case notes were fed into the computer, its diagnostic prediction proved accurate in 77% of the 301 patients, and when details of the outpatient endoscopy were added to the house surgeon's case notes, the computer's diagnostic accuracy rose to 84.7%. It is suggested that computer-aided analysis may have a limited part to play in discriminating between the common causes of lower gastrointestinal disorders in routine clinical practice.", "contents": "Computer-aided diagnosis of lower gastrointestinal tract disorders. This paper reports a survey of human and computer-aided diagnosis in a prospective consecutive series of 301 patients admitted to the hospital with lower gastrointestinal tract disease. At initial outpatient contact (at which time endoscopy was customarily performed), the clinicians' diagnostic accuracy was 64.5%. After biopsy, radiology, and other investigative procedures, the clinicians' preoperative diagnostic accuracy rose t0 82.7%. When data from the house surgeon's case notes were fed into the computer, its diagnostic prediction proved accurate in 77% of the 301 patients, and when details of the outpatient endoscopy were added to the house surgeon's case notes, the computer's diagnostic accuracy rose to 84.7%. It is suggested that computer-aided analysis may have a limited part to play in discriminating between the common causes of lower gastrointestinal disorders in routine clinical practice."}
{"id": "PMID:1090480", "title": "Pathophysiology of Shigella diarrhea in the rhesus monkey: intestinal transport, morphological, and bacteriological studies.", "content": "In contrast to the \"toxigenic diarrheas\" caused by Vibrio cholerae and Escherichia coli, the site and mechanism of fluid loss in shigellosis are unknown. The occurrence of watery diarrhea in shigellosis suggests involvement of the small bowel. Therefore, jejunal, ileal, and colonic water and electrolyte transport was studied in Shigella flexneri 2a-infected monkeys. Infected animals fell into three groups: dysentery alone, diarrhea alone, or diarrhea and dysentery. In controls, net water, sodium, and chloride absorption was seen in the jejunum, ileum, and colon. All infected animals demonstrated diminished colonic absorption or net colonic secretion. In monkeys with dysentery alone, this was the only transport defect observed. In contrast, animals with diarrhea either alone or in combination with dysentery, exhibited net jejunum secretion. Ileal transport was normal in all animals. A severe colitis with intramucosal shigellae was seen in all symptomatic animals. In the jejunum or ileum, however, morphological changes were minimal and bacterial invasion was not seen. Therefore, unlike the \"toxigenic\" diarrheas,\" shigellosis is both a small and large intestinal disease. Mucosal invasion of the colon is essential to the development of a morphological and transport defect. Dysentery results from a colonic transport defect, while diarrhea is secondary to jejunal secretion superimposed on the defect in colonic absorption.", "contents": "Pathophysiology of Shigella diarrhea in the rhesus monkey: intestinal transport, morphological, and bacteriological studies. In contrast to the \"toxigenic diarrheas\" caused by Vibrio cholerae and Escherichia coli, the site and mechanism of fluid loss in shigellosis are unknown. The occurrence of watery diarrhea in shigellosis suggests involvement of the small bowel. Therefore, jejunal, ileal, and colonic water and electrolyte transport was studied in Shigella flexneri 2a-infected monkeys. Infected animals fell into three groups: dysentery alone, diarrhea alone, or diarrhea and dysentery. In controls, net water, sodium, and chloride absorption was seen in the jejunum, ileum, and colon. All infected animals demonstrated diminished colonic absorption or net colonic secretion. In monkeys with dysentery alone, this was the only transport defect observed. In contrast, animals with diarrhea either alone or in combination with dysentery, exhibited net jejunum secretion. Ileal transport was normal in all animals. A severe colitis with intramucosal shigellae was seen in all symptomatic animals. In the jejunum or ileum, however, morphological changes were minimal and bacterial invasion was not seen. Therefore, unlike the \"toxigenic\" diarrheas,\" shigellosis is both a small and large intestinal disease. Mucosal invasion of the colon is essential to the development of a morphological and transport defect. Dysentery results from a colonic transport defect, while diarrhea is secondary to jejunal secretion superimposed on the defect in colonic absorption."}
{"id": "PMID:1090481", "title": "Comparison of secretory and histological effects of shigella and cholera enterotoxins in rabbit jejunum.", "content": "The purpose of this study was to compare the actions of shigella toxin and cholera toxin to determine if mucosal damage is a prerequisite for shigella toxin-induced secretion. The secretory response to maximal doses of cholera toxin and shigella toxin were compared. The maximal rate of secretion and the electrolyte and protein concentration of the intestinal fluid were similar for both toxins. On the other hand, the time of onset after exposure to the toxin was 105 min for shigella toxin and 15 to 30 min for cholera toxin. In addition, cholera toxin-induced secretion was associated with depletion of goblet cell mucus, whereas no change was seen in association with the response to shigella toxin. Other than goblet cell depletion, there were no histological differences between loops secreting in response to cholera toxin and to shigella toxin. Finally, the secretory effect of the toxins are not additive. These studies suggest that, in spite of apparent differences in the patterns of secretory response to the two toxins, they may share a rate-limiting step in the secretory process.", "contents": "Comparison of secretory and histological effects of shigella and cholera enterotoxins in rabbit jejunum. The purpose of this study was to compare the actions of shigella toxin and cholera toxin to determine if mucosal damage is a prerequisite for shigella toxin-induced secretion. The secretory response to maximal doses of cholera toxin and shigella toxin were compared. The maximal rate of secretion and the electrolyte and protein concentration of the intestinal fluid were similar for both toxins. On the other hand, the time of onset after exposure to the toxin was 105 min for shigella toxin and 15 to 30 min for cholera toxin. In addition, cholera toxin-induced secretion was associated with depletion of goblet cell mucus, whereas no change was seen in association with the response to shigella toxin. Other than goblet cell depletion, there were no histological differences between loops secreting in response to cholera toxin and to shigella toxin. Finally, the secretory effect of the toxins are not additive. These studies suggest that, in spite of apparent differences in the patterns of secretory response to the two toxins, they may share a rate-limiting step in the secretory process."}
{"id": "PMID:1090491", "title": "A study on the effect of certain compound during elimination of plasmids in Escherichia coli.", "content": "Of several chemicals tested on the elimination of plasmids from Escherichia coli K-12, the compound designated ICR-170 was most effective, applied at 100 mu g/ml, the effect being comparable to that of acriflavin. It had no effect on the elimination of the R1 plasmid from Escherichia coli JC 5455.", "contents": "A study on the effect of certain compound during elimination of plasmids in Escherichia coli. Of several chemicals tested on the elimination of plasmids from Escherichia coli K-12, the compound designated ICR-170 was most effective, applied at 100 mu g/ml, the effect being comparable to that of acriflavin. It had no effect on the elimination of the R1 plasmid from Escherichia coli JC 5455."}
{"id": "PMID:1090493", "title": "DNA synthesis - dependent cell division of Escherichia coli 15 TAU after arginine and uracil starvation.", "content": "Extensive cell division after synchronization of Escherichia coli 15 TAU by arginine and uracil starvation occurs only when DNA synthesis is permitted to proceed by at least a short pulse of thymine applied between 30 and 60 min after transfer of synchronized culture to thymine-free medium with arginine and uracil. The time schedule of synchronized cell division in dependence on the schedule of intervals of DNA synthesis and inhibition of DNA synthesis was determined. The termination of replication cycles which were not completed to the very end during arginine and uracil starvation seems to be the decisive event for subsequent cell division after synchronization.", "contents": "DNA synthesis - dependent cell division of Escherichia coli 15 TAU after arginine and uracil starvation. Extensive cell division after synchronization of Escherichia coli 15 TAU by arginine and uracil starvation occurs only when DNA synthesis is permitted to proceed by at least a short pulse of thymine applied between 30 and 60 min after transfer of synchronized culture to thymine-free medium with arginine and uracil. The time schedule of synchronized cell division in dependence on the schedule of intervals of DNA synthesis and inhibition of DNA synthesis was determined. The termination of replication cycles which were not completed to the very end during arginine and uracil starvation seems to be the decisive event for subsequent cell division after synchronization."}
{"id": "PMID:1090515", "title": "Enhancement of Moloney sarcoma virus-induced tumor growth by concanavalin A.", "content": "Adult mice injected intraperitoneally with concanavalin A (con-A) commencing with the onset of 6mm. Moloney sarcoma virus (MSV)-induced tumors showed a marked enhancement of tumor growth compared to untreated control mice; whereas, treatment with con-A beginning on day 0 relative to MSV inoculation had no effect. Antibody production as measured by indirect membrane fluorescence was not affected by con-A. Since in vivo administered con-A has a specific suppressive effect on T-lymphocytes it is suggested that the primary immunologic response to MSV antigens is a function of T-cells.", "contents": "Enhancement of Moloney sarcoma virus-induced tumor growth by concanavalin A. Adult mice injected intraperitoneally with concanavalin A (con-A) commencing with the onset of 6mm. Moloney sarcoma virus (MSV)-induced tumors showed a marked enhancement of tumor growth compared to untreated control mice; whereas, treatment with con-A beginning on day 0 relative to MSV inoculation had no effect. Antibody production as measured by indirect membrane fluorescence was not affected by con-A. Since in vivo administered con-A has a specific suppressive effect on T-lymphocytes it is suggested that the primary immunologic response to MSV antigens is a function of T-cells."}
{"id": "PMID:1090516", "title": "Comparative mitogenic responses of T-cells and B-cells in spleens of mice of varying age.", "content": "Mouse spleen cell mitogenic responses to Phytohemagglutinin (PHA), conconavalin A (con A), staphylococcal enterotoxin B (SEB) and lipopolysaccharide (LPS) were studied as a function of age. In CD-1 mice, spleen cell DNA synthesis in response to stimulation by PHA, con A and SEB varied from 18% - 33% of adult values for the first 3 weeks of life. By 6 weeks, the PHA response was 63% of the adult response and achieved adult values by 8 weeks of life. In contrast to these T-cell responses, DNA spleen cell synthesis in response to LPS was approximately that of adults as early as 7 days after birth. When spleen cell mitogenic responses were studied in C57B1/6J mice a similar pattern emerged. C57 spleen cell responses to PHA, con A and SEB varied from 24% - 54% of adult values for the first 3 weeks of life. By 4 weeks, spleen cell responses to PHA and con A were approximately those of adults. Again, C57 spleen cell responses to LPS were equivalent to adult values as early as 7 days after birth.", "contents": "Comparative mitogenic responses of T-cells and B-cells in spleens of mice of varying age. Mouse spleen cell mitogenic responses to Phytohemagglutinin (PHA), conconavalin A (con A), staphylococcal enterotoxin B (SEB) and lipopolysaccharide (LPS) were studied as a function of age. In CD-1 mice, spleen cell DNA synthesis in response to stimulation by PHA, con A and SEB varied from 18% - 33% of adult values for the first 3 weeks of life. By 6 weeks, the PHA response was 63% of the adult response and achieved adult values by 8 weeks of life. In contrast to these T-cell responses, DNA spleen cell synthesis in response to LPS was approximately that of adults as early as 7 days after birth. When spleen cell mitogenic responses were studied in C57B1/6J mice a similar pattern emerged. C57 spleen cell responses to PHA, con A and SEB varied from 24% - 54% of adult values for the first 3 weeks of life. By 4 weeks, spleen cell responses to PHA and con A were approximately those of adults. Again, C57 spleen cell responses to LPS were equivalent to adult values as early as 7 days after birth."}
{"id": "PMID:1090517", "title": "The bursa dependence of chicken thymus-derived lymphocyte surface immunoglobulin.", "content": "Thirty-three plus or minus 5 per cent of chicken blood lymphocytes were positively labelled by radioiodinated rabbit anti-chicken light chain antibody within 7 days of exposure of autoradiographs. After thymectomy, 93 plus or minus 1 per cent of lymphocytes were positive: conversely, following bursectomy only 5 plus or minus 3 per cent of cells showed positive labelling and then at a very low level. Hence, under these conditions of short-term exposure autoradiography, only B cells carried readily demonstrable immunoglobulin. If the sensitivity of the technique was increased by long-term exposure of the autoradiographs, immunoglobulin was demonstrable on all blood lymphocytes, including the T cells. In addition, 39 plus or minus 6 per cent of thymocytes were shown to carry immunoglobulin by the more sensitive technique. In both cases, however, this T-lymphocyte surface immunoglobulin was reduced, and in some cases absent, after bursectomy. At no stage, with either technique, did the grain count on the erythrocytes reach the control level of lymphocytes treated with 125I-labelled anti-keyhole limpet haemocyanin antibody. It seems likely that T-cell immunoglobulin is not an endogenously synthesized product but is acquired indirectly from the B lymphocytes.", "contents": "The bursa dependence of chicken thymus-derived lymphocyte surface immunoglobulin. Thirty-three plus or minus 5 per cent of chicken blood lymphocytes were positively labelled by radioiodinated rabbit anti-chicken light chain antibody within 7 days of exposure of autoradiographs. After thymectomy, 93 plus or minus 1 per cent of lymphocytes were positive: conversely, following bursectomy only 5 plus or minus 3 per cent of cells showed positive labelling and then at a very low level. Hence, under these conditions of short-term exposure autoradiography, only B cells carried readily demonstrable immunoglobulin. If the sensitivity of the technique was increased by long-term exposure of the autoradiographs, immunoglobulin was demonstrable on all blood lymphocytes, including the T cells. In addition, 39 plus or minus 6 per cent of thymocytes were shown to carry immunoglobulin by the more sensitive technique. In both cases, however, this T-lymphocyte surface immunoglobulin was reduced, and in some cases absent, after bursectomy. At no stage, with either technique, did the grain count on the erythrocytes reach the control level of lymphocytes treated with 125I-labelled anti-keyhole limpet haemocyanin antibody. It seems likely that T-cell immunoglobulin is not an endogenously synthesized product but is acquired indirectly from the B lymphocytes."}
{"id": "PMID:1090518", "title": "A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.", "content": "Several methods for the dissociation of human tonsils into cell suspensions were compared. Dissociation of tonsils using 0-25 per cent trypsin gave both the largest number of total cells and the largest number of plasma cells per gram of tonsil. Lymphocytes and plasma cells were separated in a previously described isokinetic gradient of Ficoll in tissue culture medium. In the purest gradient fractions, lymphocytes were 97-2 plus or minus 1-9 per cent of nucleated cells. The purest gradient fractions contained 43-1 plus or minus 5-9 per cent plasma cells. More than 95 per cent of purified lymphocytes and plasma cells excluded Trypan Blue.", "contents": "A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium. Several methods for the dissociation of human tonsils into cell suspensions were compared. Dissociation of tonsils using 0-25 per cent trypsin gave both the largest number of total cells and the largest number of plasma cells per gram of tonsil. Lymphocytes and plasma cells were separated in a previously described isokinetic gradient of Ficoll in tissue culture medium. In the purest gradient fractions, lymphocytes were 97-2 plus or minus 1-9 per cent of nucleated cells. The purest gradient fractions contained 43-1 plus or minus 5-9 per cent plasma cells. More than 95 per cent of purified lymphocytes and plasma cells excluded Trypan Blue."}
{"id": "PMID:1090519", "title": "Proportional absorption. A method for determination of the relative specificity of antisera prepared against cells.", "content": "Antisera prepared against a complex of antigens such as a tissue cell may produce a mixture of antibodies of different specificities, affinities and types. Proportional absorption permits determination of the comparative specificity of such antisera. It is performed by absorbing the antisera with an amount of  absorbent proportional to the initial content of (usually undesired) antibody to this absorbent; the potencies of desired and undesired antibodies are then separately determined. The method has been used to determine the specificity of a conventionally raised rabbit anti-mouse leukaemia serum, relative to one prepared with leukaemia cells admixed with rabbit antiserum against normal mouse lymphocytes (to block normal antigen sites on the leukaemia cell inoculum). The latter antiserum was cells as compared to normal splenic lymphocytes.", "contents": "Proportional absorption. A method for determination of the relative specificity of antisera prepared against cells. Antisera prepared against a complex of antigens such as a tissue cell may produce a mixture of antibodies of different specificities, affinities and types. Proportional absorption permits determination of the comparative specificity of such antisera. It is performed by absorbing the antisera with an amount of  absorbent proportional to the initial content of (usually undesired) antibody to this absorbent; the potencies of desired and undesired antibodies are then separately determined. The method has been used to determine the specificity of a conventionally raised rabbit anti-mouse leukaemia serum, relative to one prepared with leukaemia cells admixed with rabbit antiserum against normal mouse lymphocytes (to block normal antigen sites on the leukaemia cell inoculum). The latter antiserum was cells as compared to normal splenic lymphocytes."}
{"id": "PMID:1090520", "title": "The effect of various immunosuppressive agents on mouse peritoneal macrophages and on the in vitro phagocytosis of Escherichia coli O4:K3:H5 and degradation of 125I-labelled HSA-antibody complexes by these cells.", "content": "Large doses of hydrocortisone, cyclophosphamide, and methotrexate injected subcutaneously, and whole-body irradiation (500 rads) caused a reduction in the number of peritoneal cells (PE cells) obtained after intraperitoneal injection of the treated mice with proteose-peptone. The same dose of cyclophosphamide and irradiation induced morphological changes in PE macrophages. There were more giant cells in the peritoneal exudates from treated mice as compared to control mice. 'Pharmacological' and larger doses of hydrocortisone, methotrexate and azathioprine or anti-lymphocyte globulin had no effect on the in vitro phagocytic capacity of proteose-peptone-stimulated mouse PE macrophages. This also applied to doses of up to 50 mg/kg of cyclophosphamide. In contrast, whole-body irradiation (500 rad) and 100 mg/kg of cyclophosphamide decreased the phagocytic capacity of mouse macrophages in vitro and reduced the ability of PE cells to degrade 125I-labelled HSA-antibody complexes in vitro. The greatest effect was noted 4-5 days after whole-body irradiation or four to five subcutaneous injections of cyclophosphamide.", "contents": "The effect of various immunosuppressive agents on mouse peritoneal macrophages and on the in vitro phagocytosis of Escherichia coli O4:K3:H5 and degradation of 125I-labelled HSA-antibody complexes by these cells. Large doses of hydrocortisone, cyclophosphamide, and methotrexate injected subcutaneously, and whole-body irradiation (500 rads) caused a reduction in the number of peritoneal cells (PE cells) obtained after intraperitoneal injection of the treated mice with proteose-peptone. The same dose of cyclophosphamide and irradiation induced morphological changes in PE macrophages. There were more giant cells in the peritoneal exudates from treated mice as compared to control mice. 'Pharmacological' and larger doses of hydrocortisone, methotrexate and azathioprine or anti-lymphocyte globulin had no effect on the in vitro phagocytic capacity of proteose-peptone-stimulated mouse PE macrophages. This also applied to doses of up to 50 mg/kg of cyclophosphamide. In contrast, whole-body irradiation (500 rad) and 100 mg/kg of cyclophosphamide decreased the phagocytic capacity of mouse macrophages in vitro and reduced the ability of PE cells to degrade 125I-labelled HSA-antibody complexes in vitro. The greatest effect was noted 4-5 days after whole-body irradiation or four to five subcutaneous injections of cyclophosphamide."}
{"id": "PMID:1090521", "title": "Inhibition of Escherichia coli by bovine colostrum and post-colostral milk. I. Complement-mediated bactericidal activity of antibodies to a serum susceptible strain of E. coli of the serotype O 111.", "content": "Bovine colostral whey diulted in unheated bovine milk or in Kolmer saline containing 5 per cent pre-colostral calf serum was bactericidal for Escherichia coli NCTC 9703 (serotype O 111). Whey diluted in saline without pre-colostral calf serum was inactive. Bactericidal activity was abolished by heating at 56 degrees for 30 minutes or by pre-incubating with N-acetyl-L-tyrosine ethylester, indicating that complement present in milk or pre-colostral calf serum was involved in the bactericidal activity. Undiluted colostral whey was inactive even in the presence of up to 20 per cent pre-colostral calf serum, probably due to a prozone effect. Milk heated at 80 degrees for 10 minutes was also shown to have a limited anti-complementary effect. Post-colostral milk from some cows had bactericidal activity, whereas similar milks from other cows were either inactive or active only after addition of pre-colostral calf serum, indicating that antibodies to E. coli or complement or both may or may not be present in the post-colostral milk of different cows. The implications of these findings in relation to the bacterial flora of the intestinal tract of neonates are discussed.", "contents": "Inhibition of Escherichia coli by bovine colostrum and post-colostral milk. I. Complement-mediated bactericidal activity of antibodies to a serum susceptible strain of E. coli of the serotype O 111. Bovine colostral whey diulted in unheated bovine milk or in Kolmer saline containing 5 per cent pre-colostral calf serum was bactericidal for Escherichia coli NCTC 9703 (serotype O 111). Whey diluted in saline without pre-colostral calf serum was inactive. Bactericidal activity was abolished by heating at 56 degrees for 30 minutes or by pre-incubating with N-acetyl-L-tyrosine ethylester, indicating that complement present in milk or pre-colostral calf serum was involved in the bactericidal activity. Undiluted colostral whey was inactive even in the presence of up to 20 per cent pre-colostral calf serum, probably due to a prozone effect. Milk heated at 80 degrees for 10 minutes was also shown to have a limited anti-complementary effect. Post-colostral milk from some cows had bactericidal activity, whereas similar milks from other cows were either inactive or active only after addition of pre-colostral calf serum, indicating that antibodies to E. coli or complement or both may or may not be present in the post-colostral milk of different cows. The implications of these findings in relation to the bacterial flora of the intestinal tract of neonates are discussed."}
{"id": "PMID:1090522", "title": "Inhibition of Escherichia coli by bovine colostrum and post-colostral milk. II. The bacteriostatic effect of lactoferrin on a serum susceptible and serum resistant strain of E. coli.", "content": "Two strains of Escherichia coli were inhibited by complement-inactivated cow serum and to a lesser extent by precolostral calf serum devoid of specific antibodies. They were not inhibited by undiluted colostral whey or milk but colostral whey became bacteriostatic after dialysis or dilution in Kolmer saline and addition of precolostral calf serum or lactoferrin. The inhibition in all these fluids was due to iron-binding proteins (transferrin or lactoferrin). Undiluted dialysed milk was not inhibitory because of its low content of lactoferrin but became inhibitory after addition of 1 mg/ml of lactoferrin. The lack of inhibition in undiluted whey is due to the high concentration of citrate in colostral whey (and milk) and it is suggested that citrate competes with the iron-binding proteins for iron and makes it availabe to the bacteria. Addition of bicarbonate, which is required for the binding of iron by transferrin and lactoferrin, can overcome the effect of citrate; hence, the bacteriostatic effect of cow serum and precolostral calf serum is due to the presence of both transferrin and bicarbonate as well as the low lefel of citrate.", "contents": "Inhibition of Escherichia coli by bovine colostrum and post-colostral milk. II. The bacteriostatic effect of lactoferrin on a serum susceptible and serum resistant strain of E. coli. Two strains of Escherichia coli were inhibited by complement-inactivated cow serum and to a lesser extent by precolostral calf serum devoid of specific antibodies. They were not inhibited by undiluted colostral whey or milk but colostral whey became bacteriostatic after dialysis or dilution in Kolmer saline and addition of precolostral calf serum or lactoferrin. The inhibition in all these fluids was due to iron-binding proteins (transferrin or lactoferrin). Undiluted dialysed milk was not inhibitory because of its low content of lactoferrin but became inhibitory after addition of 1 mg/ml of lactoferrin. The lack of inhibition in undiluted whey is due to the high concentration of citrate in colostral whey (and milk) and it is suggested that citrate competes with the iron-binding proteins for iron and makes it availabe to the bacteria. Addition of bicarbonate, which is required for the binding of iron by transferrin and lactoferrin, can overcome the effect of citrate; hence, the bacteriostatic effect of cow serum and precolostral calf serum is due to the presence of both transferrin and bicarbonate as well as the low lefel of citrate."}
{"id": "PMID:1090523", "title": "Suppression of extablished Friend virus leukemia by statolon: potentiation of statolon's leukemosuppressive activity by chlorite-oxidized oxyamylose.", "content": "Treatment of Friend virus (FV)-infected mice, 3 days after FV inoculation, with statolon, an extract of the mold Penicillium stoloniferum, induces interferon and restores immunocompetence to viral and nonviral antigens such as sheep erythrocytes. Clinical remissions are established in 20 to 70% of the infected mice. Cholorite-oxidized oxyamylose administered intraperitoneally 24 h before, 3 h before, or 3 h after statolon enhanced interferon production, but the increased number of mice protected against FV disease was more closely related to the associated enhanced synthesis of FV cytotoxic antibody. The prolonged selective immunodepression to intraperitoneal sheep erythrocytes after intraperitoneal administration of chlorite-oxidized oxyamylose-statolon appeared to be related to a stimulation in number and erythrocyte-phagocytic capacity of peritoneal macrophages. The marked activation of macrophages in FV leukemic mice after such treatment may also have contributed to the enhanced FV leukemosuppressive effects of chlorite-oxidized oxyamylose-statolon.", "contents": "Suppression of extablished Friend virus leukemia by statolon: potentiation of statolon's leukemosuppressive activity by chlorite-oxidized oxyamylose. Treatment of Friend virus (FV)-infected mice, 3 days after FV inoculation, with statolon, an extract of the mold Penicillium stoloniferum, induces interferon and restores immunocompetence to viral and nonviral antigens such as sheep erythrocytes. Clinical remissions are established in 20 to 70% of the infected mice. Cholorite-oxidized oxyamylose administered intraperitoneally 24 h before, 3 h before, or 3 h after statolon enhanced interferon production, but the increased number of mice protected against FV disease was more closely related to the associated enhanced synthesis of FV cytotoxic antibody. The prolonged selective immunodepression to intraperitoneal sheep erythrocytes after intraperitoneal administration of chlorite-oxidized oxyamylose-statolon appeared to be related to a stimulation in number and erythrocyte-phagocytic capacity of peritoneal macrophages. The marked activation of macrophages in FV leukemic mice after such treatment may also have contributed to the enhanced FV leukemosuppressive effects of chlorite-oxidized oxyamylose-statolon."}
{"id": "PMID:1090524", "title": "Immunoelectron microscopic identification and localization of Streptococcus sanguis with peroxidase-labeled antibody: localization of surface antigens in pure cultures.", "content": "An indirect method of localizing antigens with horseradish peroxidase-labeled antibody was used to identify and localize surface antigens of Streptococcus sanguis at the ultrastructural level. An electron dense layer surrounding the cell wall could be distinguished without any additional electron microscope staining. This labeled layer represents an immune complex consisting of bacterial surface antigens, specific rabbit antisera, and peroxidase-labeled goat anti-rabbit globulins. Although with undiluted antisera slight cross-reactions occurred with S. salivarius and S. miteor (mitis), these could be readily distinguished from the more intense homologous reaction by their patchiness and the difference in distribution of the label. These cross-reactions were eliminated by appropriate dilutions of the antiserum. No cross-reactions occurred with S. mutans, S. faecalis, Actinomyces species, or Bacterionema, microorganisms wents indicated that horseradish peroxidase can become non-specifically adsorbed to the membrane of certain bacterial cells. Appropriate controls must, therefore, be included for localization of membrane associated antigens with horseradish peroxidase.", "contents": "Immunoelectron microscopic identification and localization of Streptococcus sanguis with peroxidase-labeled antibody: localization of surface antigens in pure cultures. An indirect method of localizing antigens with horseradish peroxidase-labeled antibody was used to identify and localize surface antigens of Streptococcus sanguis at the ultrastructural level. An electron dense layer surrounding the cell wall could be distinguished without any additional electron microscope staining. This labeled layer represents an immune complex consisting of bacterial surface antigens, specific rabbit antisera, and peroxidase-labeled goat anti-rabbit globulins. Although with undiluted antisera slight cross-reactions occurred with S. salivarius and S. miteor (mitis), these could be readily distinguished from the more intense homologous reaction by their patchiness and the difference in distribution of the label. These cross-reactions were eliminated by appropriate dilutions of the antiserum. No cross-reactions occurred with S. mutans, S. faecalis, Actinomyces species, or Bacterionema, microorganisms wents indicated that horseradish peroxidase can become non-specifically adsorbed to the membrane of certain bacterial cells. Appropriate controls must, therefore, be included for localization of membrane associated antigens with horseradish peroxidase."}
{"id": "PMID:1090525", "title": "Regional immunosuppression induced by Plasmodium berghei yoelii infection in mice.", "content": "Plasmodium berghei yoelii infection in mice severely depressed the splenic antibody response to sheep erythrocytes but had lettle effect on antibody formation in lymph nodes.", "contents": "Regional immunosuppression induced by Plasmodium berghei yoelii infection in mice. Plasmodium berghei yoelii infection in mice severely depressed the splenic antibody response to sheep erythrocytes but had lettle effect on antibody formation in lymph nodes."}
{"id": "PMID:1090526", "title": "Growth of mycobacterium bovis (BCG) in T lymphocyte-depleted mice.", "content": "BCG Montreal (10-6 viable bacilli) injected intravenously into adult thymectomized, irradiated, and bone marrow-reconstituted (THXB) C57Bl times C3H F1 hybrid mice induced a progressive systemic infection which killed 95% of the animals within 60 days. Control mice infected with this dose of BCG did not die. The infected THXB mice failed to develop detectable levels of tuberculin hypersensitivity although they did show considerable Arthus (3 h) reactivity. The BCG-infected THXB mice lost weight progressively, and the root spleen and root lung indices increased substantially as the infection proceeded. None of the THXB mice developed an antibacterial immune response to the systemic BCG infection, and this was reflected by the continued persistence of macroscopic lung granuloma in these animals. The BCG-infected control mice developed as many surface tubercles as did the THXB animals, but the granulomas rapidly regressed in size and numbers in the normal mice. The lung changes correlated with the amount of tritiated thymidine incorporated by the lung cells in the later stages of the BCG infection. T cell depletion depressed the early splenic peak normally seen in BCG-infected controls, but, on the other hand, there was a progressive increase in lung counts in the THXB mice as the infection progressed and this late peak was not seen in the control animals. The significance of these findings is discussed in relation to the development of antituberculous immunity by BCG-infected mice.", "contents": "Growth of mycobacterium bovis (BCG) in T lymphocyte-depleted mice. BCG Montreal (10-6 viable bacilli) injected intravenously into adult thymectomized, irradiated, and bone marrow-reconstituted (THXB) C57Bl times C3H F1 hybrid mice induced a progressive systemic infection which killed 95% of the animals within 60 days. Control mice infected with this dose of BCG did not die. The infected THXB mice failed to develop detectable levels of tuberculin hypersensitivity although they did show considerable Arthus (3 h) reactivity. The BCG-infected THXB mice lost weight progressively, and the root spleen and root lung indices increased substantially as the infection proceeded. None of the THXB mice developed an antibacterial immune response to the systemic BCG infection, and this was reflected by the continued persistence of macroscopic lung granuloma in these animals. The BCG-infected control mice developed as many surface tubercles as did the THXB animals, but the granulomas rapidly regressed in size and numbers in the normal mice. The lung changes correlated with the amount of tritiated thymidine incorporated by the lung cells in the later stages of the BCG infection. T cell depletion depressed the early splenic peak normally seen in BCG-infected controls, but, on the other hand, there was a progressive increase in lung counts in the THXB mice as the infection progressed and this late peak was not seen in the control animals. The significance of these findings is discussed in relation to the development of antituberculous immunity by BCG-infected mice."}
{"id": "PMID:1090527", "title": "Control of salmonellosis pacifarin biosynthesis by iron.", "content": "Enterobacter cloacae strain SS4-56 produced salmonellosis pacifarins when grown in an iron-depleted synthetic medium to which 3.16 X 10-7 g-atom of iron had been added per liter, but did not do so when grown in iron-depleted medium supplemented with 3.16 X 10-4 g-atom of iron per liter. The addition of 3.16 X 10-4 g-atom of ferric iron per liter to a pacifarin-containing culture supernatant fluid had no significant effect upon the ability of the active pacifarins present, when administered per os, to protect mice from an otherwise fatal infection produced by a sequential injection of avirulent and virulent strains of Salmonella typhimurium.", "contents": "Control of salmonellosis pacifarin biosynthesis by iron. Enterobacter cloacae strain SS4-56 produced salmonellosis pacifarins when grown in an iron-depleted synthetic medium to which 3.16 X 10-7 g-atom of iron had been added per liter, but did not do so when grown in iron-depleted medium supplemented with 3.16 X 10-4 g-atom of iron per liter. The addition of 3.16 X 10-4 g-atom of ferric iron per liter to a pacifarin-containing culture supernatant fluid had no significant effect upon the ability of the active pacifarins present, when administered per os, to protect mice from an otherwise fatal infection produced by a sequential injection of avirulent and virulent strains of Salmonella typhimurium."}
{"id": "PMID:1090528", "title": "Phagocytosis of Cryptococcus neoformans by alveolar macrophages.", "content": "Guinea pig pulmonary macrophages phagocytized but did not kill nonencapsulated cells of Cryptococcus neoformans. The phagocytic process was inhibited by cryptococcal capsular polysaccharide. Pulmonary macrophages, activated by preinjecting heat-killed bacteria into intact animals, did not kill the engulfed yeast cells. Labeled cells of C. neoformans were neither killed nor cleared from guinea pig lungs 6 h postexposure. The results of our experiments indicate that during the first few hours after the lung is exposed to the infectious particle of C. neoformans the pulmonary macrophage does not function primarily to kill engulfed yeast cells. We believe that a rapid yet transient acute inflammatory response probably plays a major role in this process during the first few hours after C. neoformans enters the lung.", "contents": "Phagocytosis of Cryptococcus neoformans by alveolar macrophages. Guinea pig pulmonary macrophages phagocytized but did not kill nonencapsulated cells of Cryptococcus neoformans. The phagocytic process was inhibited by cryptococcal capsular polysaccharide. Pulmonary macrophages, activated by preinjecting heat-killed bacteria into intact animals, did not kill the engulfed yeast cells. Labeled cells of C. neoformans were neither killed nor cleared from guinea pig lungs 6 h postexposure. The results of our experiments indicate that during the first few hours after the lung is exposed to the infectious particle of C. neoformans the pulmonary macrophage does not function primarily to kill engulfed yeast cells. We believe that a rapid yet transient acute inflammatory response probably plays a major role in this process during the first few hours after C. neoformans enters the lung."}
{"id": "PMID:1090529", "title": "Correlation between phagocytic activity and metabolic response of polymorphonuclear leukocytes toward different strains of Escherichia coli.", "content": "The bactericidal activity, the phagocytic capacity, and the metabolic stimulation of polymorphonuclear leukocytes challenged with different strains of Escherichia coli were studied. It was found that only two strains out of 10 tested stimulated the oxygen consumption and carbohydrate metabolism of leukocytes and were readily killed by the phagocytes. The lack of killing of the other eight strains was shown to be due to absent or poor phagocytosis rather than to resistance to intracellular killing. Evidence was presented that the surface K antigen plays an important role in conferring antiphagocytic properties to some strains of E. coli. It was suggested that K antigen acts by interfering with the early step of the phagocytic process, that is, the attachment step.", "contents": "Correlation between phagocytic activity and metabolic response of polymorphonuclear leukocytes toward different strains of Escherichia coli. The bactericidal activity, the phagocytic capacity, and the metabolic stimulation of polymorphonuclear leukocytes challenged with different strains of Escherichia coli were studied. It was found that only two strains out of 10 tested stimulated the oxygen consumption and carbohydrate metabolism of leukocytes and were readily killed by the phagocytes. The lack of killing of the other eight strains was shown to be due to absent or poor phagocytosis rather than to resistance to intracellular killing. Evidence was presented that the surface K antigen plays an important role in conferring antiphagocytic properties to some strains of E. coli. It was suggested that K antigen acts by interfering with the early step of the phagocytic process, that is, the attachment step."}
{"id": "PMID:1090530", "title": "Immunosuppression by hydroxystilbamidine isethionate, a lysosome-stabilizing, anti-proteolytic, antifungal drug.", "content": "Hydroxystilbamidine (HSB) is a potent suppressor of the plaque-forming cell response of mice injected with heterologous erythrocytes. HSB, given in varying doses and injection schedules, suppressed both the primary and secondary immune responses to bovine serum albumin. Apparently the effect is not simply a toxic effect on spleen cells, because there was no appreciable difference in cell numbers between control and HSB-treated mice. The effect of HSB was most apparent in the early phase of the immune respone.", "contents": "Immunosuppression by hydroxystilbamidine isethionate, a lysosome-stabilizing, anti-proteolytic, antifungal drug. Hydroxystilbamidine (HSB) is a potent suppressor of the plaque-forming cell response of mice injected with heterologous erythrocytes. HSB, given in varying doses and injection schedules, suppressed both the primary and secondary immune responses to bovine serum albumin. Apparently the effect is not simply a toxic effect on spleen cells, because there was no appreciable difference in cell numbers between control and HSB-treated mice. The effect of HSB was most apparent in the early phase of the immune respone."}
{"id": "PMID:1090531", "title": "Genetic basis of toxin production and pathogenesis in Vibrio cholerae: evidence against phage conversion.", "content": "The pathogenicity of Vibrio cholerae strains \"cured\" of \"Kappa-type\" phage was not significantly altered relative to that of their \"Kappa\" lysogenic parental strains. Unlike Corynebacterium diphtheriae, the capacity of V. cholerae to produce exotoxin was not stimulated as a consequence of active phage multiplication. Toxin production in cultures in which Kappa-type phage multiplication was initiated either by inducing Kappa lysogens or by infecting naturally occurring or \"cured\" Kappa-sensitive strains was greatly reduced compared to normally growing control cultures. Kappa-sensitive El Tor strain Mak 757 and a Kappa lysogen derived from it did not differ in their capacity to colonize ligated rabbit ileal loops nor in their sensitivites to ultraviolet radiation, acidic pH, or osmotic shock. We conclude that Kappa-type phages do not directly affect the pathogenicity of these V. cholerae strains.", "contents": "Genetic basis of toxin production and pathogenesis in Vibrio cholerae: evidence against phage conversion. The pathogenicity of Vibrio cholerae strains \"cured\" of \"Kappa-type\" phage was not significantly altered relative to that of their \"Kappa\" lysogenic parental strains. Unlike Corynebacterium diphtheriae, the capacity of V. cholerae to produce exotoxin was not stimulated as a consequence of active phage multiplication. Toxin production in cultures in which Kappa-type phage multiplication was initiated either by inducing Kappa lysogens or by infecting naturally occurring or \"cured\" Kappa-sensitive strains was greatly reduced compared to normally growing control cultures. Kappa-sensitive El Tor strain Mak 757 and a Kappa lysogen derived from it did not differ in their capacity to colonize ligated rabbit ileal loops nor in their sensitivites to ultraviolet radiation, acidic pH, or osmotic shock. We conclude that Kappa-type phages do not directly affect the pathogenicity of these V. cholerae strains."}
{"id": "PMID:1090532", "title": "Cytotoxic effect of Mycoplasma fermentans on mouse thymocytes.", "content": "The in vitro response of mouse thymocytes to various mycoplasmas was evaluated. Cultures of thymus cells from BALB mice were prepared in Earle minimal essential medium with 10 per cent fetal calf serum. After an initial drop in viability, cell populations stabilized at approximately 10-5 viable cells/ml for 3 to 5 days. Concentration of 10-6 to 10-8 colony-forming units of toxic isolates of Mycoplasma fermentans per ml killed over 50 per cent of these cells in a dose-dependent fashion. Four other mycoplasmas (M. pneumoniae, M. hominis, M. arthritidis and a nontoxic strain of M. fermentans) did not induce cytotoxicity of mouse thymocytes. Toxic isolates of M. fermentans multiplied in the presence of thymus cells as they were being inactivated. However, nonviable membrane preparations of these mycoplasma were also toxic, indicating that growth of the organisms is not a prerequisite for the toxic effect. The relevance of these findings for the isolation and identification of the membrane-associated toxic factor is discussed.", "contents": "Cytotoxic effect of Mycoplasma fermentans on mouse thymocytes. The in vitro response of mouse thymocytes to various mycoplasmas was evaluated. Cultures of thymus cells from BALB mice were prepared in Earle minimal essential medium with 10 per cent fetal calf serum. After an initial drop in viability, cell populations stabilized at approximately 10-5 viable cells/ml for 3 to 5 days. Concentration of 10-6 to 10-8 colony-forming units of toxic isolates of Mycoplasma fermentans per ml killed over 50 per cent of these cells in a dose-dependent fashion. Four other mycoplasmas (M. pneumoniae, M. hominis, M. arthritidis and a nontoxic strain of M. fermentans) did not induce cytotoxicity of mouse thymocytes. Toxic isolates of M. fermentans multiplied in the presence of thymus cells as they were being inactivated. However, nonviable membrane preparations of these mycoplasma were also toxic, indicating that growth of the organisms is not a prerequisite for the toxic effect. The relevance of these findings for the isolation and identification of the membrane-associated toxic factor is discussed."}
{"id": "PMID:1090533", "title": "Characterization of Escherichia coli obtained from newborn calves with diarrhea.", "content": "A total of 373 isolates of Escherichia coli were obtained from one or more calves with diarrhea in 155 herds during the 1974 calving season in Montana. Sixty-seven (18 percent) of the isolates representing 59 of the 155 herds were found to be enterotoxigenic as indicated by their ability to cause distention of the calf ligated intestinal segment. The 67 isolates of enterotoxigenic E. coli (ETEC) were placed in one of six different antigenic groups based upon agglutination of formolized whole cells in KO antiserum. Eighty-seven percent (58 of 67) of the ETEC had antigen 1, 2, or 3, whereas only 2.3 per cent (7 of 310) of the non-ETEC (NETEC) had antigen 1, 2, or 3. This antigen numbering system was used for convenience and is not related to any established typing system. Antigens 1, 2, and 3 do not belong to any of the O groups 1 to 157 or K groups 1 to 93 of the International Schema. Colony color or morphology of ETEC and NETEC grown on Tergitol-7 agar with triphenyltetrazolium chloride added could not be used as an indicator of enterotoxigenicity, although there was a tendency for E. coli with the smooth and mucoid colony type to be enterotoxigenic whereas rough colonies were seldom enterotoxigenic. Among ETEC isolates with antigen 1, 2, or 3, there was good correlation between colony type and antigen number. All 13 ETEC isolates with antigen 1 had the smooth colony type, 17 of 19 ETEC isolates with antigen 2 had the smooth, mucoid colony type, and all 25 isolates of ETEC with antigen 3 had the intermediate colony type. Conversely, of the 310 isolates of NETEC, none had antigen 1 and the smooth colony type; none had antigen 2 and the smooth and mucoid colony type; and only one isolate of NETEC had antigen 3 and the intermediate colony type. Sixteen of the 67 isolates of ETEC (24 percent) were motile. Fifteen of the 16 motile isolates of ETEC had the intermediate colony type, and none of the ETEC with smooth or smooth and mucoid colonies were motile.", "contents": "Characterization of Escherichia coli obtained from newborn calves with diarrhea. A total of 373 isolates of Escherichia coli were obtained from one or more calves with diarrhea in 155 herds during the 1974 calving season in Montana. Sixty-seven (18 percent) of the isolates representing 59 of the 155 herds were found to be enterotoxigenic as indicated by their ability to cause distention of the calf ligated intestinal segment. The 67 isolates of enterotoxigenic E. coli (ETEC) were placed in one of six different antigenic groups based upon agglutination of formolized whole cells in KO antiserum. Eighty-seven percent (58 of 67) of the ETEC had antigen 1, 2, or 3, whereas only 2.3 per cent (7 of 310) of the non-ETEC (NETEC) had antigen 1, 2, or 3. This antigen numbering system was used for convenience and is not related to any established typing system. Antigens 1, 2, and 3 do not belong to any of the O groups 1 to 157 or K groups 1 to 93 of the International Schema. Colony color or morphology of ETEC and NETEC grown on Tergitol-7 agar with triphenyltetrazolium chloride added could not be used as an indicator of enterotoxigenicity, although there was a tendency for E. coli with the smooth and mucoid colony type to be enterotoxigenic whereas rough colonies were seldom enterotoxigenic. Among ETEC isolates with antigen 1, 2, or 3, there was good correlation between colony type and antigen number. All 13 ETEC isolates with antigen 1 had the smooth colony type, 17 of 19 ETEC isolates with antigen 2 had the smooth, mucoid colony type, and all 25 isolates of ETEC with antigen 3 had the intermediate colony type. Conversely, of the 310 isolates of NETEC, none had antigen 1 and the smooth colony type; none had antigen 2 and the smooth and mucoid colony type; and only one isolate of NETEC had antigen 3 and the intermediate colony type. Sixteen of the 67 isolates of ETEC (24 percent) were motile. Fifteen of the 16 motile isolates of ETEC had the intermediate colony type, and none of the ETEC with smooth or smooth and mucoid colonies were motile."}
{"id": "PMID:1090534", "title": "Interactions between Mycoplasma pneumoniae and guinea pig complement.", "content": "The \"toxic\" effect of guinea pig serum (GPS) on Mycoplasma pneumoniae cells was tested under various conditions, using rounding and killing of the cells as test systems. Both activities could be inhibited by heat inactivation (56 C, 30 min). Killing required both Ca2+ and Mg2+, rounding only Mg2+. Both activities were temperature dependent and no rounding or killing occurred at 4C. Incomplete complement sequences with natural of artificial defects in C1, C4, or C6 resulted in lost or reduced killing. The rounding activity was only slightly affected. Anti-C3 antiserum blocked both phenomena; incubation of GPS with 10 mg of inulin per ml reduced the rounding activity, and the same treatment of GPS deficient in C4 inhibited rounding totally. Properdin factor D was shown to be necessary for rounding by GPS, with defects in either C1 or C4. By immune adherence bound C3b could be demonstrated on M. pneumoniae cells after GPS treatment, no antibodies against M. pneumoniae could be found in GPS by immune fluorescence. The results give evidence for complement being the toxic factor in GPS. Efficient killing requires the intact complement sequence. Furthermore, M. pneumoniae cells are able to activate the alternate pathway of complement. Activation of this pathway results in rounding of the cells, which are partly able to recover after this reaction. Biological consequences for the mycoplasmas are death or damage and possibly opsonization, even in the absence of specific antibodies. The host, too, is possibly affected by products of the reaction. The interaction of M. pneumoniae and complement could be involved in the early stages of the development of M. pneumoniae disease.", "contents": "Interactions between Mycoplasma pneumoniae and guinea pig complement. The \"toxic\" effect of guinea pig serum (GPS) on Mycoplasma pneumoniae cells was tested under various conditions, using rounding and killing of the cells as test systems. Both activities could be inhibited by heat inactivation (56 C, 30 min). Killing required both Ca2+ and Mg2+, rounding only Mg2+. Both activities were temperature dependent and no rounding or killing occurred at 4C. Incomplete complement sequences with natural of artificial defects in C1, C4, or C6 resulted in lost or reduced killing. The rounding activity was only slightly affected. Anti-C3 antiserum blocked both phenomena; incubation of GPS with 10 mg of inulin per ml reduced the rounding activity, and the same treatment of GPS deficient in C4 inhibited rounding totally. Properdin factor D was shown to be necessary for rounding by GPS, with defects in either C1 or C4. By immune adherence bound C3b could be demonstrated on M. pneumoniae cells after GPS treatment, no antibodies against M. pneumoniae could be found in GPS by immune fluorescence. The results give evidence for complement being the toxic factor in GPS. Efficient killing requires the intact complement sequence. Furthermore, M. pneumoniae cells are able to activate the alternate pathway of complement. Activation of this pathway results in rounding of the cells, which are partly able to recover after this reaction. Biological consequences for the mycoplasmas are death or damage and possibly opsonization, even in the absence of specific antibodies. The host, too, is possibly affected by products of the reaction. The interaction of M. pneumoniae and complement could be involved in the early stages of the development of M. pneumoniae disease."}
{"id": "PMID:1090535", "title": "Opsonin-reversible resistance of Mycoplasma pneumoniae to in vitro phagocytosis by alveolar macrophages.", "content": "Several species of mycoplasmas are responsible for respiratory disease in animals and man. As yet, little is known about the interaction of these pathogens with alveolar macrophages, one of the primary components of pulmonary resistance to infections. The present study was undertaken to develop an in vitro model to examine this organism-cell interaction, using a human pathogen, mycoplasma pneumoniae, and normal guinea pig alveolar macrophages. During a 24-h incubation of M. pneumoniae with a monolayer of macrophages, mycoplasmas were found to attach directly to the surface of the cells without inducing significant phagocytosis. Ultrastructurally, the organisms appeared bound to the cell membrane by their characteristic attachment organelles. Only after the addition of specific anti-mycoplasma serum were cells able to engulf attached and surrounding organisms. These data suggest that the interaction of M. pneumoniae and alveolar macrophages is a potentially important aspect of disease pathogenesis, and immune factors which might alter this interaction merit further examination.", "contents": "Opsonin-reversible resistance of Mycoplasma pneumoniae to in vitro phagocytosis by alveolar macrophages. Several species of mycoplasmas are responsible for respiratory disease in animals and man. As yet, little is known about the interaction of these pathogens with alveolar macrophages, one of the primary components of pulmonary resistance to infections. The present study was undertaken to develop an in vitro model to examine this organism-cell interaction, using a human pathogen, mycoplasma pneumoniae, and normal guinea pig alveolar macrophages. During a 24-h incubation of M. pneumoniae with a monolayer of macrophages, mycoplasmas were found to attach directly to the surface of the cells without inducing significant phagocytosis. Ultrastructurally, the organisms appeared bound to the cell membrane by their characteristic attachment organelles. Only after the addition of specific anti-mycoplasma serum were cells able to engulf attached and surrounding organisms. These data suggest that the interaction of M. pneumoniae and alveolar macrophages is a potentially important aspect of disease pathogenesis, and immune factors which might alter this interaction merit further examination."}
{"id": "PMID:1090536", "title": "Calf ligated intestinal segment test to detect enterotoxigenic Escherichia coli.", "content": "The ligated intestinal segment test in the young calf was utilized to establish the enterotoxigenicity of approximately 600 Escherichia coli isolates obtained from fecal specimens intestinal contents of calves with diarrheal disease. One hundred and fifty isolates were routinely tested in a single calf. There was little problem with false-positive reactions. False-negative reactions normally occurred only if the isolate was tested in the posterior 3 m of the small intestine. The ligated small intestine of the calf was distended by E. coli isolates of bovine, porcine, and human origin. Use of the calf ligated intestinal segment test is recommended to determine the enterotoxigenicity of E. coli isolates of bovine origin.", "contents": "Calf ligated intestinal segment test to detect enterotoxigenic Escherichia coli. The ligated intestinal segment test in the young calf was utilized to establish the enterotoxigenicity of approximately 600 Escherichia coli isolates obtained from fecal specimens intestinal contents of calves with diarrheal disease. One hundred and fifty isolates were routinely tested in a single calf. There was little problem with false-positive reactions. False-negative reactions normally occurred only if the isolate was tested in the posterior 3 m of the small intestine. The ligated small intestine of the calf was distended by E. coli isolates of bovine, porcine, and human origin. Use of the calf ligated intestinal segment test is recommended to determine the enterotoxigenicity of E. coli isolates of bovine origin."}
{"id": "PMID:1090537", "title": "Variations in pulmonary antibacterial defenses among experimental animals.", "content": "Intrapulmonary killing of Staphylococcus aureus proceeded at equal rates in guinea pigs, hamsters, rats, and mice. In the lung of guinea pigs and hamsters, Proteus mirabilis was killed at virtually the same rate as S. aureus as compared with half the rate of inactivation in the lungs of rats and mice.", "contents": "Variations in pulmonary antibacterial defenses among experimental animals. Intrapulmonary killing of Staphylococcus aureus proceeded at equal rates in guinea pigs, hamsters, rats, and mice. In the lung of guinea pigs and hamsters, Proteus mirabilis was killed at virtually the same rate as S. aureus as compared with half the rate of inactivation in the lungs of rats and mice."}
{"id": "PMID:1090538", "title": "Antibody and cell-mediated immunity in phytohemagglutinin-treated chickens.", "content": "The effects of phytohemagglutinin (PHA) on humoral and homograft immunity in young chickens were studied. Intravenous (IV) injections of PHA stimulated the antibody production while intraperitoneal (IP) injections suppressed the antibody production. IP injected PHA failed to prolong the survival of the homografts. The route and time of PHA administration must be considered when evaluating the influence which PHA exerts on homograft survival and hemagglutinin production in the chiicken. The results obtained in this study suggest that there may be two different target cell lines involved in PHA's action. The reduced antibody production following IP injections of PHA may be due to its interference with the peritoneal macrophages and T cells and in the case ov IV administration PHA may be activating both macrophages and T cells. Possibly, there may be a difference in the effective PHA concentration after IP versus IV administration to account for different responses observed.", "contents": "Antibody and cell-mediated immunity in phytohemagglutinin-treated chickens. The effects of phytohemagglutinin (PHA) on humoral and homograft immunity in young chickens were studied. Intravenous (IV) injections of PHA stimulated the antibody production while intraperitoneal (IP) injections suppressed the antibody production. IP injected PHA failed to prolong the survival of the homografts. The route and time of PHA administration must be considered when evaluating the influence which PHA exerts on homograft survival and hemagglutinin production in the chiicken. The results obtained in this study suggest that there may be two different target cell lines involved in PHA's action. The reduced antibody production following IP injections of PHA may be due to its interference with the peritoneal macrophages and T cells and in the case ov IV administration PHA may be activating both macrophages and T cells. Possibly, there may be a difference in the effective PHA concentration after IP versus IV administration to account for different responses observed."}
{"id": "PMID:1090539", "title": "Effect of carrageenan on the development of hypersensitivity (Schistosoma mansoni egg) and foreign body (divinyl-benzene copolymer beads and bentonite) granulomas.", "content": "The effect of carrageenan on the development of hypersensitivity and foreign body granulomas was examined. Repeated intraperitoneal injections of the polysaccharide caused a strong suppression of the primary, hypersensitivity granulomas forming around the eggs of Schistosoma mansoni worms in the lungs of mice, but had no effect on the secondary, enhanced egg granulomas in the previously sensitized animals. Carrageenan treatment virtually abolished the granulomatous reactions forming around plastic beads or bentonite particles. It is concluded that carrageenan exerted its suppressive effect not through macrophage toxicity but rather by affecting plasma factors which regulate vascular permeability and cellular traffic during the process of inflammation.", "contents": "Effect of carrageenan on the development of hypersensitivity (Schistosoma mansoni egg) and foreign body (divinyl-benzene copolymer beads and bentonite) granulomas. The effect of carrageenan on the development of hypersensitivity and foreign body granulomas was examined. Repeated intraperitoneal injections of the polysaccharide caused a strong suppression of the primary, hypersensitivity granulomas forming around the eggs of Schistosoma mansoni worms in the lungs of mice, but had no effect on the secondary, enhanced egg granulomas in the previously sensitized animals. Carrageenan treatment virtually abolished the granulomatous reactions forming around plastic beads or bentonite particles. It is concluded that carrageenan exerted its suppressive effect not through macrophage toxicity but rather by affecting plasma factors which regulate vascular permeability and cellular traffic during the process of inflammation."}
{"id": "PMID:1090540", "title": "Effect of concanavalin A on immunologic abnormalities of New Zealand (NZB/W) mice.", "content": "The administration of Concanavalin A (Con A) to young New Zealand (NZB/W) mice has been previously found to render them tolerable to BGG. The corresponding mechanism is unknown but is consistent with other observations that Con A is capable of activating a suppressor T cell population. We have further explored this possibility by giving New Zealand mice Con A from 1 month until 11 months of age and monitoring several known immunologic abnormalities. Con-A-treated mice, in contrast to littermate controls, were found to have lower anti-DNA levels, less proteinuria, a better response to primary sheep red cell immunization and normally rejected skin allografts.", "contents": "Effect of concanavalin A on immunologic abnormalities of New Zealand (NZB/W) mice. The administration of Concanavalin A (Con A) to young New Zealand (NZB/W) mice has been previously found to render them tolerable to BGG. The corresponding mechanism is unknown but is consistent with other observations that Con A is capable of activating a suppressor T cell population. We have further explored this possibility by giving New Zealand mice Con A from 1 month until 11 months of age and monitoring several known immunologic abnormalities. Con-A-treated mice, in contrast to littermate controls, were found to have lower anti-DNA levels, less proteinuria, a better response to primary sheep red cell immunization and normally rejected skin allografts."}
{"id": "PMID:1090541", "title": "Intracellular localization of the migration inhibitory factor (MIF) in a long-term human lymphoid cell line.", "content": "Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: beta-glucuronidase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activity was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of exterase. No such correlation with MIF activity could be demonstrated for beta-glucuronidase or LDH.", "contents": "Intracellular localization of the migration inhibitory factor (MIF) in a long-term human lymphoid cell line. Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: beta-glucuronidase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activity was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of exterase. No such correlation with MIF activity could be demonstrated for beta-glucuronidase or LDH."}
{"id": "PMID:1090542", "title": "Drug-induced tolerance: selective induction with immunosuppressive drugs and their synergistic interaction.", "content": "Drug-induced tolerance to polyinosinic-polycytidylic acid was studied in NZB/W mice. A single injection of cyclophosphamide (CY) or methotrexate (MTX) induced tolerance when given 24 h after antigen, whereas azathioprine (AZ) and methylprednisolone (MP) did not. However, MP and MTX each synergized with a nontolerogenic dose of CY. In addition, MP and AZ further reduced the tolerance induced by low but tolerogenic doses of CY. Drug combinations in the induction of tolerance may have wide application in the fields of clinical immunology and transplantation.", "contents": "Drug-induced tolerance: selective induction with immunosuppressive drugs and their synergistic interaction. Drug-induced tolerance to polyinosinic-polycytidylic acid was studied in NZB/W mice. A single injection of cyclophosphamide (CY) or methotrexate (MTX) induced tolerance when given 24 h after antigen, whereas azathioprine (AZ) and methylprednisolone (MP) did not. However, MP and MTX each synergized with a nontolerogenic dose of CY. In addition, MP and AZ further reduced the tolerance induced by low but tolerogenic doses of CY. Drug combinations in the induction of tolerance may have wide application in the fields of clinical immunology and transplantation."}
{"id": "PMID:1090543", "title": "Rosette formation with sheep erythrocytes--a possible T-cell marker in the pig.", "content": "A proportion of pig lymphocytes form rosettes with sheep erythrocytes. Factors affecting their demonstration have been investigated, and a standard technique defined. Rosette-forming lymphocytes lacked surface immunoglobulin detected by immunofluorescence and formation of rosettes was not inhibited by anti-immunoglobulin or anti-PLA sera, but was by anti-thymus serum. Of 18 species' erythrocytes tested only sheep, Barbary sheep and Mouflon erythrocytes formed rosettes in similar percentages. Fetal sheep erythrocytes formed no rosettes at 6o days of gestation and developed adult levels by term. Rosettes were formed by the majority of thymus cells, by only few bone marrow cells and by intermediate proportions of cells in other lymphoid tissues correlating with the probable order of T cell content. In pig fetuses, thymus contained postnatal levels of rosette-forming cells by 69 days, when such cells were not detected in other tissues. These data support the contention that SRBC rosettes are formed by T lymphocytes.", "contents": "Rosette formation with sheep erythrocytes--a possible T-cell marker in the pig. A proportion of pig lymphocytes form rosettes with sheep erythrocytes. Factors affecting their demonstration have been investigated, and a standard technique defined. Rosette-forming lymphocytes lacked surface immunoglobulin detected by immunofluorescence and formation of rosettes was not inhibited by anti-immunoglobulin or anti-PLA sera, but was by anti-thymus serum. Of 18 species' erythrocytes tested only sheep, Barbary sheep and Mouflon erythrocytes formed rosettes in similar percentages. Fetal sheep erythrocytes formed no rosettes at 6o days of gestation and developed adult levels by term. Rosettes were formed by the majority of thymus cells, by only few bone marrow cells and by intermediate proportions of cells in other lymphoid tissues correlating with the probable order of T cell content. In pig fetuses, thymus contained postnatal levels of rosette-forming cells by 69 days, when such cells were not detected in other tissues. These data support the contention that SRBC rosettes are formed by T lymphocytes."}
{"id": "PMID:1090544", "title": "Subcellular fractions from dermis and epidermis in contact sensitization of guinea pigs to 1-chloro-2,4-dinitrobenzene.", "content": "Subcellular fractions were prepared from the epidermis of guinea pigs which had been painted with 1-chloro-2,4 dinitrobenzene 14 h previously. The microsomal fraction showed less activity than other fractions in the MIF test. Similarly, the microsomal fraction from the dermis of skin irritated with croton oil before DNCB painting was relatively inactive. However, the microsomal fractions from the epidermis and more especially from the dermis of skin painted with DNCB while undergoing allergic contact dermatitis from pentadecylcatechol showed significant MIF activities.", "contents": "Subcellular fractions from dermis and epidermis in contact sensitization of guinea pigs to 1-chloro-2,4-dinitrobenzene. Subcellular fractions were prepared from the epidermis of guinea pigs which had been painted with 1-chloro-2,4 dinitrobenzene 14 h previously. The microsomal fraction showed less activity than other fractions in the MIF test. Similarly, the microsomal fraction from the dermis of skin irritated with croton oil before DNCB painting was relatively inactive. However, the microsomal fractions from the epidermis and more especially from the dermis of skin painted with DNCB while undergoing allergic contact dermatitis from pentadecylcatechol showed significant MIF activities."}
{"id": "PMID:1090545", "title": "Use of immunofluorescent localization in the normal guinea pig eye to differentiate three autoantisera.", "content": "Three distinct immunofluorescent patterns were demonstrated in normal guinea pig eyes by autoantisera prepared to three different ocular tissue preparations. Anti-U serum (antiserum to ureal homogenate) exhibited specific fluorescence in the area of Bruch's membrane. Anti-P serum (antiserum to a suspension of the sedimentable portion of retina homogenate) exhibited specific fluorescence in the outer segments of the photoreceptor cells. Anti-S serum (antiserum to retinal homogenate extract) demonstrated specific fluorescence in the outer portion of the retina extending from the outer plexiform layer to the outer segments of the photoreceptor cells. The ability of anti-S and anti-P sera immunofluorescent activities to cross species lines was demonstrated.", "contents": "Use of immunofluorescent localization in the normal guinea pig eye to differentiate three autoantisera. Three distinct immunofluorescent patterns were demonstrated in normal guinea pig eyes by autoantisera prepared to three different ocular tissue preparations. Anti-U serum (antiserum to ureal homogenate) exhibited specific fluorescence in the area of Bruch's membrane. Anti-P serum (antiserum to a suspension of the sedimentable portion of retina homogenate) exhibited specific fluorescence in the outer segments of the photoreceptor cells. Anti-S serum (antiserum to retinal homogenate extract) demonstrated specific fluorescence in the outer portion of the retina extending from the outer plexiform layer to the outer segments of the photoreceptor cells. The ability of anti-S and anti-P sera immunofluorescent activities to cross species lines was demonstrated."}
{"id": "PMID:1090546", "title": "Observations on rabbit thymocytes and peripheral T cells. II. Rosette formation with rabbit erythrocytes.", "content": "The affinity of rabbit thymocytes and a proportion of lymphocytes for homologous and autologous erythrocytes has been investigated. Thymocytes were found to rosette strongly with untreated, washed erythrocytes, although reactions on peripheral lymphocytes from blood and lymph nodes were weaker. Enzyme treatment of the erythrocytes was used in attempts to improve reactions with peripheral lymphocytes. While neuraminidase-treated cells showed no increased reactivity, erythrocytes treated with papain were more firmly bound and reacted with greater numbers of blood and lymph node cells. Evidence was obtained to show that peripheral lymphocytes which have an affinity for papain-treated erythrocytes belong to the thymus-derived T cell population.", "contents": "Observations on rabbit thymocytes and peripheral T cells. II. Rosette formation with rabbit erythrocytes. The affinity of rabbit thymocytes and a proportion of lymphocytes for homologous and autologous erythrocytes has been investigated. Thymocytes were found to rosette strongly with untreated, washed erythrocytes, although reactions on peripheral lymphocytes from blood and lymph nodes were weaker. Enzyme treatment of the erythrocytes was used in attempts to improve reactions with peripheral lymphocytes. While neuraminidase-treated cells showed no increased reactivity, erythrocytes treated with papain were more firmly bound and reacted with greater numbers of blood and lymph node cells. Evidence was obtained to show that peripheral lymphocytes which have an affinity for papain-treated erythrocytes belong to the thymus-derived T cell population."}
{"id": "PMID:1090547", "title": "Shared antigenicity between chicken brain and bursa of fabricius.", "content": "Antisera to chicken brain, thymus and bursa of Fabricius were prepared in rabbits and rendered organ-specific by appropriate absorptions. Brain was shown by immunofluorescence and immune adherence to have common antigenicity with the bursa of Fabricius but not with the thymus.", "contents": "Shared antigenicity between chicken brain and bursa of fabricius. Antisera to chicken brain, thymus and bursa of Fabricius were prepared in rabbits and rendered organ-specific by appropriate absorptions. Brain was shown by immunofluorescence and immune adherence to have common antigenicity with the bursa of Fabricius but not with the thymus."}
{"id": "PMID:1090548", "title": "[Theories of cognitive disorder in schizophrenia based on psychological experiments (author's transl)].", "content": "Over the last few years, psychological experiments have provided a major contribution to research in schizophrenia. Historical, practical and theoretical grounds led to investigations mainly in the field of cognitive disorder. Independent psychological theories of schizophrenia emerged from the body of experimental results, at first restricted to mental performance of the patients but later generalized to center on the total problem of schizophrenia. In accordance with psychiatric thinking, psychologists favoured cognitive deficit as the essential schizophrenic characteristic which would possibly generate the basic symptomatology. Apart from methodological considerations, the main question arising is if this feature is truly typical of schizophrenia. The problem of the different concepts of schizophrenia has to be considered in this connection. A survey of theories of schizophrenia which are sufficiently consolidated by psychological experiments reveals that two lines of theoretical reasoning exist: one based on specific cognitive disorder, and the other where this is not the case. Encompassed by the first classification are the considerations of Bannister, Rodnick and Germezy, Shakow, McReynolds, Silvermann, L.J. Chapman, Payne, Mednick, and Epstein, as well as the interference theories (with the exception of Callaway's version)..", "contents": "[Theories of cognitive disorder in schizophrenia based on psychological experiments (author's transl)]. Over the last few years, psychological experiments have provided a major contribution to research in schizophrenia. Historical, practical and theoretical grounds led to investigations mainly in the field of cognitive disorder. Independent psychological theories of schizophrenia emerged from the body of experimental results, at first restricted to mental performance of the patients but later generalized to center on the total problem of schizophrenia. In accordance with psychiatric thinking, psychologists favoured cognitive deficit as the essential schizophrenic characteristic which would possibly generate the basic symptomatology. Apart from methodological considerations, the main question arising is if this feature is truly typical of schizophrenia. The problem of the different concepts of schizophrenia has to be considered in this connection. A survey of theories of schizophrenia which are sufficiently consolidated by psychological experiments reveals that two lines of theoretical reasoning exist: one based on specific cognitive disorder, and the other where this is not the case. Encompassed by the first classification are the considerations of Bannister, Rodnick and Germezy, Shakow, McReynolds, Silvermann, L.J. Chapman, Payne, Mednick, and Epstein, as well as the interference theories (with the exception of Callaway's version).."}
{"id": "PMID:1090549", "title": "A plea for a discipline of health and medical evaluation.", "content": "There is an expanding need for quantitative information to evaluate health and medical care procedures. Existing methods for designing and carrying out evaluation studies need to be improved to provide better techniques for answering questions on efficacy of health and medical procedures, and in providing a more adequate information base on which to set healthy policy. This paper reviews some of the problems in the design and conduct of evaluation research, and makes a plea for creation of a special discipline to support and develop the field of health and medical care evaluation.", "contents": "A plea for a discipline of health and medical evaluation. There is an expanding need for quantitative information to evaluate health and medical care procedures. Existing methods for designing and carrying out evaluation studies need to be improved to provide better techniques for answering questions on efficacy of health and medical procedures, and in providing a more adequate information base on which to set healthy policy. This paper reviews some of the problems in the design and conduct of evaluation research, and makes a plea for creation of a special discipline to support and develop the field of health and medical care evaluation."}
{"id": "PMID:1090552", "title": "Gamma-ray-induced thymine damage in the DNA in coliphage phi chi 174 and in E. coli.", "content": "The radiation chemical reactivity of thymine residues has been compared for single-stranded phi chi 174 DNA free in solution and in the intact bacteriophage, as well as for native E. coli DNA in solution and inside the cell. The gamma-ray-induced release of [3H]H2O from thymine-methyl[3H] was measured to assess the reactivity of the thymine methyl group. Formation of ring-saturated products of the 5,6-dihydroxy-dihydrothymine type (see article) was determined by an alkali-acid degradation assay. It was observed that the formation of that the formation of [3H]H2O was suppressed by a factor of 9 in intact phi chi 174 bacteriophage compared to phi chi 174 DNA, whereas the formation of (see article) was only slightly suppressed in the phage. For E. coli cells the formation of [3H]H29 was reduced 790-fold compared with free E. coli DNA, and (see article) formation was reduced 140-fold. The extents of survival after gamma-irradiation of phi chi 174 phage plaque-forming ability and E. coli colony-forming ability were also determined. Under the conditions used in these experiments, a linear relationship was observed between the loss of biological activity and the formation of thymine damage for both phi chi 174 phage and E. coli cells.", "contents": "Gamma-ray-induced thymine damage in the DNA in coliphage phi chi 174 and in E. coli. The radiation chemical reactivity of thymine residues has been compared for single-stranded phi chi 174 DNA free in solution and in the intact bacteriophage, as well as for native E. coli DNA in solution and inside the cell. The gamma-ray-induced release of [3H]H2O from thymine-methyl[3H] was measured to assess the reactivity of the thymine methyl group. Formation of ring-saturated products of the 5,6-dihydroxy-dihydrothymine type (see article) was determined by an alkali-acid degradation assay. It was observed that the formation of that the formation of [3H]H2O was suppressed by a factor of 9 in intact phi chi 174 bacteriophage compared to phi chi 174 DNA, whereas the formation of (see article) was only slightly suppressed in the phage. For E. coli cells the formation of [3H]H29 was reduced 790-fold compared with free E. coli DNA, and (see article) formation was reduced 140-fold. The extents of survival after gamma-irradiation of phi chi 174 phage plaque-forming ability and E. coli colony-forming ability were also determined. Under the conditions used in these experiments, a linear relationship was observed between the loss of biological activity and the formation of thymine damage for both phi chi 174 phage and E. coli cells."}
{"id": "PMID:1090553", "title": "Carotid endarterectomy utilizing the exclusion clamp.", "content": "Carotid endarterectomy is useful in the treatment of the uncomplicated pre-stroke patient. Use of the exclusion clamp makes possible a short period of carotid occlusion without the use of either internal or external shunts. Forty-two patients were operated on using this technic.", "contents": "Carotid endarterectomy utilizing the exclusion clamp. Carotid endarterectomy is useful in the treatment of the uncomplicated pre-stroke patient. Use of the exclusion clamp makes possible a short period of carotid occlusion without the use of either internal or external shunts. Forty-two patients were operated on using this technic."}
{"id": "PMID:1090556", "title": "Hydroureter and hydronephrosis of pregnancy presenting as acute obstetric or surgical emergencies.", "content": "The unusual clinical course of three cases of dilatation of ureters and renal pelvises in pregnancy was reported. The presenting symptom in each case was acute abdominal pain, which was at first attributed to other surgical or obstetric emergencies like premature separation of placenta, acute appendicitis and acute hydramnion.", "contents": "Hydroureter and hydronephrosis of pregnancy presenting as acute obstetric or surgical emergencies. The unusual clinical course of three cases of dilatation of ureters and renal pelvises in pregnancy was reported. The presenting symptom in each case was acute abdominal pain, which was at first attributed to other surgical or obstetric emergencies like premature separation of placenta, acute appendicitis and acute hydramnion."}
{"id": "PMID:1090557", "title": "Cornea-damaging proteases of Serratia marcescens.", "content": "Fractionation of the culture supernatant fluids of Serratia marcescens, strain BG, by ammonium sulfate precipitation, isoelectric focusing, ion-exchange chromatography, hydroxyapatite adsorption chromatography, and gel filtration failed to separate the rabbit cornea-damaging activity and the in vitro protease activity of the preparations. Two proteases having similar molecular weights (44,000), estimated by gel filtration, and isoelectric points of approximately 5.0 and 5.3 were obtained free of detectable amounts of other known extracellular serratia enzymes. Heating a mixture of the two proteases for 15 minutes at 60 degrees C. resulted in complete loss of protease and cornea-damaging activities. Production of protease and cornea-damaging activities was inhibited by ammonium sulfate. The results support the conclusion that extracellular proteases produced in vitro by S. marcescens can elicit rapid and extensive damage to the rabbit cornea.", "contents": "Cornea-damaging proteases of Serratia marcescens. Fractionation of the culture supernatant fluids of Serratia marcescens, strain BG, by ammonium sulfate precipitation, isoelectric focusing, ion-exchange chromatography, hydroxyapatite adsorption chromatography, and gel filtration failed to separate the rabbit cornea-damaging activity and the in vitro protease activity of the preparations. Two proteases having similar molecular weights (44,000), estimated by gel filtration, and isoelectric points of approximately 5.0 and 5.3 were obtained free of detectable amounts of other known extracellular serratia enzymes. Heating a mixture of the two proteases for 15 minutes at 60 degrees C. resulted in complete loss of protease and cornea-damaging activities. Production of protease and cornea-damaging activities was inhibited by ammonium sulfate. The results support the conclusion that extracellular proteases produced in vitro by S. marcescens can elicit rapid and extensive damage to the rabbit cornea."}
{"id": "PMID:1090558", "title": "Fluorescein diffusion in the human optic disc.", "content": "The characteristics of the transcapillary transfer of fluorescein dye in the optic disc of healthy individuals has been studied. A diffusible fluorescein dye and a nondiffusible reference substance, indocyanine green (ICG), which was assumed to remain in the capillaries, were injected into the circulatory system. The time courses of the concentrations of the two dyes in the optic disc were determined by simultaneously recording the fluorescence intensity of fluorescein and the infrared absorption by ICG with a fundus reflectometer. The difference between the fluorescein concentration curve and the reference ICG curve is a measure of the accumulation of fluorescein in the disc tissue. Our measurements indicate that fluorescein dye does not diffuse across the capillaries in the optic disc. The accumulation of fluorescein in the disc only starts at about one minute after the injection and seems to be due to diffusion of the dye from the surrounding choroid. The time constant of this diffusion process was found to be approximately one minute.", "contents": "Fluorescein diffusion in the human optic disc. The characteristics of the transcapillary transfer of fluorescein dye in the optic disc of healthy individuals has been studied. A diffusible fluorescein dye and a nondiffusible reference substance, indocyanine green (ICG), which was assumed to remain in the capillaries, were injected into the circulatory system. The time courses of the concentrations of the two dyes in the optic disc were determined by simultaneously recording the fluorescence intensity of fluorescein and the infrared absorption by ICG with a fundus reflectometer. The difference between the fluorescein concentration curve and the reference ICG curve is a measure of the accumulation of fluorescein in the disc tissue. Our measurements indicate that fluorescein dye does not diffuse across the capillaries in the optic disc. The accumulation of fluorescein in the disc only starts at about one minute after the injection and seems to be due to diffusion of the dye from the surrounding choroid. The time constant of this diffusion process was found to be approximately one minute."}
{"id": "PMID:1090559", "title": "Bilateral slipped upper femoral epiphysis: a rare manifestation of renal osteodystrophy. Case report with discussion of its pathogenesis.", "content": "Bilateral slipped upper femoral epiphysis is a rare manifestation of renal osteodystrophy. A case of bilateral slipped femoral epiphysis in an 18-year old male suffering from chronic renal failure due to oligomeganephronic renal hypoplasia with profound signs of renal osteodystrophy is presented. Serum growth hormone levels were high, while those of urinary 17-ketosteroids were decreased. Following subtotal parathyroidectomy, the progression of the process leading to slipped epiphysis was halted with closure of the epiphyses. The patient was subsequently treated with chronic hemodialysis for several months, after which successful renal transplantation was performed. The pathogenesis of renal osteodystrophy leading to slipped epiphysis is discussed and attention drawn to the fact that bilateral slipped femoral epiphysis may be the first clinical sign of chronic renal insufficiency.", "contents": "Bilateral slipped upper femoral epiphysis: a rare manifestation of renal osteodystrophy. Case report with discussion of its pathogenesis. Bilateral slipped upper femoral epiphysis is a rare manifestation of renal osteodystrophy. A case of bilateral slipped femoral epiphysis in an 18-year old male suffering from chronic renal failure due to oligomeganephronic renal hypoplasia with profound signs of renal osteodystrophy is presented. Serum growth hormone levels were high, while those of urinary 17-ketosteroids were decreased. Following subtotal parathyroidectomy, the progression of the process leading to slipped epiphysis was halted with closure of the epiphyses. The patient was subsequently treated with chronic hemodialysis for several months, after which successful renal transplantation was performed. The pathogenesis of renal osteodystrophy leading to slipped epiphysis is discussed and attention drawn to the fact that bilateral slipped femoral epiphysis may be the first clinical sign of chronic renal insufficiency."}
{"id": "PMID:1090560", "title": "The management of urologic complications after kidney transplantation.", "content": "Urologic complications occurred in four (20%) of 20 kidney transplants performed in 18 patients. The complications encountered were acute hemorrhage from the distal end of the ureter, a calyceal fistula, a ureteral stricture with associated fungus infection, and obstructive uropathy. In two of the four cases it was necessary to sacrifice the transplant.", "contents": "The management of urologic complications after kidney transplantation. Urologic complications occurred in four (20%) of 20 kidney transplants performed in 18 patients. The complications encountered were acute hemorrhage from the distal end of the ureter, a calyceal fistula, a ureteral stricture with associated fungus infection, and obstructive uropathy. In two of the four cases it was necessary to sacrifice the transplant."}
{"id": "PMID:1090565", "title": "On the history of basic fuchsin and aldehyde-Schiff reactions from 1862 to 1935.", "content": "The nature of products formed by aldehydes and Schiff's reagent, whether they are sulfonic or sulfinic acid compounds, has been the subject of much discussion. It seems therefore timely to review early studies of aldehyde-Schiff reactions, including the history of pararosanilin and related dyes. Dyes of the basic fuchsin group have been studied extensively since 1862, and their triphenylmethane structure was established in 1878. The currently used structural formulas were introduced around the turn of the century. Reactions of basic fuchsin with aldehydes, with and without addition of SO2, were investigated by Schiff in the 1860's i.e. before the structure of these dyes was known. In 1900 Prud'homme showed that the reaction products of basic fuschsin, sodium bisulfite and formaldehyde are alkylated and sulfonated derivatives of the parent compound; further chemical studies indicated attachment of the sulfonic acid group to the carbon atom of the aldehyde. Prud'homme's findings were repeatedly confirmed during the following decades. Wieland and Scheuing were apparently unaware of these studies and introduced the sulfinic acid theory in 1921; furthermore, they considered substitution at two amino group of Schiff's reagent essential for formation of the colored compound. However, later chemical and spectroscopic studies showed no evidence of-N-sulfinic acids but supported the sulfonic acid theory of Prud'homme.", "contents": "On the history of basic fuchsin and aldehyde-Schiff reactions from 1862 to 1935. The nature of products formed by aldehydes and Schiff's reagent, whether they are sulfonic or sulfinic acid compounds, has been the subject of much discussion. It seems therefore timely to review early studies of aldehyde-Schiff reactions, including the history of pararosanilin and related dyes. Dyes of the basic fuchsin group have been studied extensively since 1862, and their triphenylmethane structure was established in 1878. The currently used structural formulas were introduced around the turn of the century. Reactions of basic fuchsin with aldehydes, with and without addition of SO2, were investigated by Schiff in the 1860's i.e. before the structure of these dyes was known. In 1900 Prud'homme showed that the reaction products of basic fuschsin, sodium bisulfite and formaldehyde are alkylated and sulfonated derivatives of the parent compound; further chemical studies indicated attachment of the sulfonic acid group to the carbon atom of the aldehyde. Prud'homme's findings were repeatedly confirmed during the following decades. Wieland and Scheuing were apparently unaware of these studies and introduced the sulfinic acid theory in 1921; furthermore, they considered substitution at two amino group of Schiff's reagent essential for formation of the colored compound. However, later chemical and spectroscopic studies showed no evidence of-N-sulfinic acids but supported the sulfonic acid theory of Prud'homme."}
{"id": "PMID:1090568", "title": "Detection of nonintegrated plasmid deoxyribonucleic acid in the folded chromosome of Escherichia coli: physiochemical approach to studying the unit of segregation.", "content": "Physiocochemical evidence presented indicates plasmid deoxyribonucleic acid (DNA) can associate with host chromosome without linear insertion of the former into the latter. This conclusion is based on the observation that covalently closed circular (CCC) plasmid DNA can cosediment with undegraded host chromosome in a neutral sucrose gradient. When F plus bacteria are lysed under conditions that preserve chromosome, approximately 90% of CCC F sex factor plasmid (about 1% of the total DNA) is found in folded chromosomes sedimenting at rates between 1,500 and 4,000s. The remaining 10% of the CCC F DNA sediments at the rate (80S) indicative of the free CCC plasmid form. Reconstruction experiments in which 80S, CCC F DNA is added to F plus or F minus bacteria before cell lysis show that exogenous F DNA does not associate with folded chromosomes. In F plus bacteria, F plasmid is harbored at a level of one or two copies per chromosomal equivalent. In bacteria producing colicin E1, the genetic determinant of this colicin, the Col E1 plasmid, is harbored at levels of 10 to 13 copies per chromosomal equivalent; yet, greater than 90% of these plasmids do not cosediment with the 1,800S species of folded chromosome. However, preliminary evidence suggests one or two Col E1 plasmids may associate with the 1,800S folded chromosome. Based on evidence presented in this and other papers, we postulate F plasmid can link to folded chromosome because the physicochemical structure of the plasmid resembles a supercoiled region of the chromosome and, therefore, is able to interact with the ribonucleic acid that stabilizes the folded chromosome structure. Implications of this model for F plasmid replication and segregation are discussed.", "contents": "Detection of nonintegrated plasmid deoxyribonucleic acid in the folded chromosome of Escherichia coli: physiochemical approach to studying the unit of segregation. Physiocochemical evidence presented indicates plasmid deoxyribonucleic acid (DNA) can associate with host chromosome without linear insertion of the former into the latter. This conclusion is based on the observation that covalently closed circular (CCC) plasmid DNA can cosediment with undegraded host chromosome in a neutral sucrose gradient. When F plus bacteria are lysed under conditions that preserve chromosome, approximately 90% of CCC F sex factor plasmid (about 1% of the total DNA) is found in folded chromosomes sedimenting at rates between 1,500 and 4,000s. The remaining 10% of the CCC F DNA sediments at the rate (80S) indicative of the free CCC plasmid form. Reconstruction experiments in which 80S, CCC F DNA is added to F plus or F minus bacteria before cell lysis show that exogenous F DNA does not associate with folded chromosomes. In F plus bacteria, F plasmid is harbored at a level of one or two copies per chromosomal equivalent. In bacteria producing colicin E1, the genetic determinant of this colicin, the Col E1 plasmid, is harbored at levels of 10 to 13 copies per chromosomal equivalent; yet, greater than 90% of these plasmids do not cosediment with the 1,800S species of folded chromosome. However, preliminary evidence suggests one or two Col E1 plasmids may associate with the 1,800S folded chromosome. Based on evidence presented in this and other papers, we postulate F plasmid can link to folded chromosome because the physicochemical structure of the plasmid resembles a supercoiled region of the chromosome and, therefore, is able to interact with the ribonucleic acid that stabilizes the folded chromosome structure. Implications of this model for F plasmid replication and segregation are discussed."}
{"id": "PMID:1090569", "title": "Reinitiation of deoxyribonucleic acid synthesis by deoxyribonucleic acid initiation mutants of Escherichia coli: role of ribonucleic acid synthesis, protein synthesis, and cell division.", "content": "The dnaA and dnaC genes are thought to code for two proteins required for the initiation of chromosomal deoxyribonucleic acid replication in Escherichia coli. When a strain carrying a mutation in either of these genes is shifted from a permissive to a restrictive temperature, chromosome replication ceases after a period of residual synthesis. When the strains are reincubated at the permissive temperature, replication again resumes after a short lag. This reinitiation does not require either protein synthesis (as measured by resistance to chloramphenicol) or ribonucleic acid synthesis (as measured by resistance to rifampin). Thus, if there is a requirement for the synthesis of a specific ribonucleic acid to initiate deoxyribonucleic acid replication, this ribonucleic acid can be synthesized prior to the time of initiation and is relatively stable. Furthermore, the synthesis of this hypothetical ribonucleic acid does not require either the dnaA of dnaC gene products. The buildup at the restrictive temperature of the potential to reinitiate deoxyribonucleic acid synthesis at the permissive temperature shows rather complex kinetics the buildup roughly parallels the rate of mass increase of the culture for at least the first mass doubling at the restrictive temperature. At later times there appears to be a gradual loss of initiation potential despite a continued increase in mass. Under optimal conditions the increase in initiation potential can equal, but not exceed, the increase in cell division at the restrictive temperature. These results are most easily interpreted according to models that postulate a relationship between the initiation of deoxyribonucleic acid synthesis and the processes leading to cell division.", "contents": "Reinitiation of deoxyribonucleic acid synthesis by deoxyribonucleic acid initiation mutants of Escherichia coli: role of ribonucleic acid synthesis, protein synthesis, and cell division. The dnaA and dnaC genes are thought to code for two proteins required for the initiation of chromosomal deoxyribonucleic acid replication in Escherichia coli. When a strain carrying a mutation in either of these genes is shifted from a permissive to a restrictive temperature, chromosome replication ceases after a period of residual synthesis. When the strains are reincubated at the permissive temperature, replication again resumes after a short lag. This reinitiation does not require either protein synthesis (as measured by resistance to chloramphenicol) or ribonucleic acid synthesis (as measured by resistance to rifampin). Thus, if there is a requirement for the synthesis of a specific ribonucleic acid to initiate deoxyribonucleic acid replication, this ribonucleic acid can be synthesized prior to the time of initiation and is relatively stable. Furthermore, the synthesis of this hypothetical ribonucleic acid does not require either the dnaA of dnaC gene products. The buildup at the restrictive temperature of the potential to reinitiate deoxyribonucleic acid synthesis at the permissive temperature shows rather complex kinetics the buildup roughly parallels the rate of mass increase of the culture for at least the first mass doubling at the restrictive temperature. At later times there appears to be a gradual loss of initiation potential despite a continued increase in mass. Under optimal conditions the increase in initiation potential can equal, but not exceed, the increase in cell division at the restrictive temperature. These results are most easily interpreted according to models that postulate a relationship between the initiation of deoxyribonucleic acid synthesis and the processes leading to cell division."}
{"id": "PMID:1090570", "title": "Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids.", "content": "Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies reveal that the plasmids coding for the production of heat stable and heat labile enteroxtoxins of Escherichia coli, regardless of their origin, have a majority of their polynucleotide sequences in common, but are not related in any significant way to those plasmids coding for the synthesis of only ST toxin. The heat stable and heat labile plasmids also share a significant degree of their polynucleotide sequences with plasmids of the FI and FII incompatibility groups, but not with R factors belonging to the I, N, W, P, or X incompatibility groups.", "contents": "Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids. Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies reveal that the plasmids coding for the production of heat stable and heat labile enteroxtoxins of Escherichia coli, regardless of their origin, have a majority of their polynucleotide sequences in common, but are not related in any significant way to those plasmids coding for the synthesis of only ST toxin. The heat stable and heat labile plasmids also share a significant degree of their polynucleotide sequences with plasmids of the FI and FII incompatibility groups, but not with R factors belonging to the I, N, W, P, or X incompatibility groups."}
{"id": "PMID:1090571", "title": "Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions.", "content": "We have developed a convenient and specific positive selection for long deletions through the gal region of the chromosomes of Salmonella typhimurium and Escherichia coli. Through simultaneous selection for mutations in the two closely linked genes, gal and chlA, a variety of deletions of varying length, some extending through as much as 1 min of the chromosome, could be readily obtained. Many of these deletions resulted in the loss of a gene, which we named dhb, concerned with the ability of the bacterium to synthesize the iron chelating agent enterobactin. The selection was adapted for the screening of mutagens for their ability to generate long deletions in the bacterial deoxyribonucleic acid. Forty agents were screened for this capability. Nitrous acid, previously reported to be an efficient mutagen for this purpose, increased the frequency of deletion mutations 50-fold in our system. Three others, nitrogen mustard, mitomycin C, and fast neutrons, were shown to increase the frequency of long deletions between five- and eightfold. The remainder were found to be incapable of generating these deletions.", "contents": "Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions. We have developed a convenient and specific positive selection for long deletions through the gal region of the chromosomes of Salmonella typhimurium and Escherichia coli. Through simultaneous selection for mutations in the two closely linked genes, gal and chlA, a variety of deletions of varying length, some extending through as much as 1 min of the chromosome, could be readily obtained. Many of these deletions resulted in the loss of a gene, which we named dhb, concerned with the ability of the bacterium to synthesize the iron chelating agent enterobactin. The selection was adapted for the screening of mutagens for their ability to generate long deletions in the bacterial deoxyribonucleic acid. Forty agents were screened for this capability. Nitrous acid, previously reported to be an efficient mutagen for this purpose, increased the frequency of deletion mutations 50-fold in our system. Three others, nitrogen mustard, mitomycin C, and fast neutrons, were shown to increase the frequency of long deletions between five- and eightfold. The remainder were found to be incapable of generating these deletions."}
{"id": "PMID:1090572", "title": "Assay and regulation of S-adenosylmethionine synthetase in Saccharomyces cerevisiae and Candida utilis.", "content": "A simple and sensitive assay for S-adenosylmethionine (SAM) synthetase is described which depends on the quantitative separation of the product, [14CH3]S-adenosylmethionine, from the substrate, L-[14CH3]methionine, on a Bio-Rex 70 column. L-Methionine protects the enzyme during preparation of cell extracts by sonic treatment but causes repression of enzyme activity during growth of Candida utilis. The presence of 5 mM methionine in the growth medium repressed SAM synthetase specific activity threefold compared to the specific acitivity of the enzyme isolated from cells grown in unsupplemented medium. Conversely, the presence of methionine in the growth medium resulted in an 80-fold increase in the intracellular concentration of SAM as compared to the Sam accumulated intracellularly in unsupplemented cultures.", "contents": "Assay and regulation of S-adenosylmethionine synthetase in Saccharomyces cerevisiae and Candida utilis. A simple and sensitive assay for S-adenosylmethionine (SAM) synthetase is described which depends on the quantitative separation of the product, [14CH3]S-adenosylmethionine, from the substrate, L-[14CH3]methionine, on a Bio-Rex 70 column. L-Methionine protects the enzyme during preparation of cell extracts by sonic treatment but causes repression of enzyme activity during growth of Candida utilis. The presence of 5 mM methionine in the growth medium repressed SAM synthetase specific activity threefold compared to the specific acitivity of the enzyme isolated from cells grown in unsupplemented medium. Conversely, the presence of methionine in the growth medium resulted in an 80-fold increase in the intracellular concentration of SAM as compared to the Sam accumulated intracellularly in unsupplemented cultures."}
{"id": "PMID:1090573", "title": "Autolytic enzymes in hyphae of Aspergillus nidulans: their action on old and newly formed walls.", "content": "Walls, purified from hyphae of the ascomycete Aspergillus nidulans, autolyzed on incubation and liberated glucose, mannose, galactose, N-acetylglucosamine, and soluble oligosaccharides. Digestion proceeded at linear rates until approximately 3% of the wall polymers had been hydrolyzed and then slowed markedly. The change in the rate of autolysis was not due to loss of enzyme activity but was caused by the disappearance of a fraction of the wall which was highly susceptible to digestion. Radioactive labeling showed that this fraction was the newly formed wall. The new wall was highly susceptible to enzyme action both when it was deposited at the apex in growing hyphae or when deposited laterally in hyphae treated with cycloheximide. The relations between wall modification and apical growth are discussed.", "contents": "Autolytic enzymes in hyphae of Aspergillus nidulans: their action on old and newly formed walls. Walls, purified from hyphae of the ascomycete Aspergillus nidulans, autolyzed on incubation and liberated glucose, mannose, galactose, N-acetylglucosamine, and soluble oligosaccharides. Digestion proceeded at linear rates until approximately 3% of the wall polymers had been hydrolyzed and then slowed markedly. The change in the rate of autolysis was not due to loss of enzyme activity but was caused by the disappearance of a fraction of the wall which was highly susceptible to digestion. Radioactive labeling showed that this fraction was the newly formed wall. The new wall was highly susceptible to enzyme action both when it was deposited at the apex in growing hyphae or when deposited laterally in hyphae treated with cycloheximide. The relations between wall modification and apical growth are discussed."}
{"id": "PMID:1090574", "title": "Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.", "content": "A composite plasmid has been constructed in vitro from colicin E1 factor (mass of 4.2 megadaltons [Md]) and nontransmissible resistance factor RSF 1010 (mass, 5.5. Md) deoxyribonucleic acids (DNAs) by the sequential action of Escherichia coli endonuclease (RI (Eco RI) and T4 phage DNA ligase on the covalently closed circular forms of the constituents. The composite plasmid was selected and amplified in vivo by sequential transformation of E. coli C600 with the ligated mixture and selection of transformants in medium containing streptomycin plus colicin E1, followed by amplification in the presence of chloramphenicol and purification of the extracted plasmid by dye-buoyant density gradient centrifugation in ethidium bromide-cesium chloride solution. Treatment of the composite plasmid with Eco RI yielded two fragments with mobilities corresponding to the linear forms of the parental plasmids, whereas Serratia marscesens endonuclease R (SmaR), which introduces a single scission in the colicin E1 factor but not in RSF 1010, convErted the composite plasmid to a single linear molecule (mass, 9.7 Md). Sequential degradation of colicin E1 factor with Sma R and Eco RI produced two fragments with masses of 3.5 and 0.7 Md; sequential degradation of RSF 1010 produced only one fragment (due to the cleavage with Eco RI), and sequential degradation of the composite plasmid produced the expected three fragments--an RSF 1010 Eco RI linear and the two expected products from the colicin E1 factor moiety. The composite plasmid conferred on the host cell resistance to streptomycin, sulfonamides, and colicin E1, but colicin E1 itself was not synthesized. In contrast, colicin E1 was synthesized by cells containing simultaneously both colicin E1 factor and RSF 1010 as separate entities. In the presence of chloramphenicol, the composite plasmid continued to replicate for 6 h. whereas replication of RSF 1010 and chromosomal DNA stopped within 2 h. Continued replication in the presence of chloramphenicol suggests that the replicator of the colicin E1 factor is functional in the composite plasmid.", "contents": "Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. A composite plasmid has been constructed in vitro from colicin E1 factor (mass of 4.2 megadaltons [Md]) and nontransmissible resistance factor RSF 1010 (mass, 5.5. Md) deoxyribonucleic acids (DNAs) by the sequential action of Escherichia coli endonuclease (RI (Eco RI) and T4 phage DNA ligase on the covalently closed circular forms of the constituents. The composite plasmid was selected and amplified in vivo by sequential transformation of E. coli C600 with the ligated mixture and selection of transformants in medium containing streptomycin plus colicin E1, followed by amplification in the presence of chloramphenicol and purification of the extracted plasmid by dye-buoyant density gradient centrifugation in ethidium bromide-cesium chloride solution. Treatment of the composite plasmid with Eco RI yielded two fragments with mobilities corresponding to the linear forms of the parental plasmids, whereas Serratia marscesens endonuclease R (SmaR), which introduces a single scission in the colicin E1 factor but not in RSF 1010, convErted the composite plasmid to a single linear molecule (mass, 9.7 Md). Sequential degradation of colicin E1 factor with Sma R and Eco RI produced two fragments with masses of 3.5 and 0.7 Md; sequential degradation of RSF 1010 produced only one fragment (due to the cleavage with Eco RI), and sequential degradation of the composite plasmid produced the expected three fragments--an RSF 1010 Eco RI linear and the two expected products from the colicin E1 factor moiety. The composite plasmid conferred on the host cell resistance to streptomycin, sulfonamides, and colicin E1, but colicin E1 itself was not synthesized. In contrast, colicin E1 was synthesized by cells containing simultaneously both colicin E1 factor and RSF 1010 as separate entities. In the presence of chloramphenicol, the composite plasmid continued to replicate for 6 h. whereas replication of RSF 1010 and chromosomal DNA stopped within 2 h. Continued replication in the presence of chloramphenicol suggests that the replicator of the colicin E1 factor is functional in the composite plasmid."}
{"id": "PMID:1090575", "title": "Recombination and the Escherichia coli K-12 sex factor F.", "content": "Recombination between two Flac tra minus elements to give Flac tra plus recombinants was measured in Rec plus and Rec minus strains of Escherichia coli K-12. Polar tra mutations were used to increase the proportion of tra plus recombinants among the parental Flac tra minus elements transferred by complementation. The kinetics, measured in a rec plus strain, showed that recombination began about 1 h after the initiation of mating and was completed about 1 h later. Recombination was abolished in a recA minus strain, reduced by two-thirds in a recF minus strain, and unaffected in recB minus and recC minus strains. It is proposed that the part not due to the RecF pathway results from a RecBC- and RecF-independent system for formation of single-stranded joins. One such join could be followed either by transfer and a site-specific recombination event, or by a second single-stranded join and then transfer: in either case replication and inheritance of the recombinant molecule would be dependent upon the F transfer replication system. Chromosome mobilization by an F' element was normal in a recB plus recF minus strain, and was reduced only fourfold in a recB minus recF plus strain: in the latter strain, both the RecF pathway and the system for single-stranded joins may have contributed to mobilization. Measurement of post-conjugational chromosomal recombination in exponential-phase recipient cells carrying surface exclusion-deficient Flac mutants indicated that F does not itself determine a generalized recombination system able to replace the RecA plus product or the RecBC and RecF pathways.", "contents": "Recombination and the Escherichia coli K-12 sex factor F. Recombination between two Flac tra minus elements to give Flac tra plus recombinants was measured in Rec plus and Rec minus strains of Escherichia coli K-12. Polar tra mutations were used to increase the proportion of tra plus recombinants among the parental Flac tra minus elements transferred by complementation. The kinetics, measured in a rec plus strain, showed that recombination began about 1 h after the initiation of mating and was completed about 1 h later. Recombination was abolished in a recA minus strain, reduced by two-thirds in a recF minus strain, and unaffected in recB minus and recC minus strains. It is proposed that the part not due to the RecF pathway results from a RecBC- and RecF-independent system for formation of single-stranded joins. One such join could be followed either by transfer and a site-specific recombination event, or by a second single-stranded join and then transfer: in either case replication and inheritance of the recombinant molecule would be dependent upon the F transfer replication system. Chromosome mobilization by an F' element was normal in a recB plus recF minus strain, and was reduced only fourfold in a recB minus recF plus strain: in the latter strain, both the RecF pathway and the system for single-stranded joins may have contributed to mobilization. Measurement of post-conjugational chromosomal recombination in exponential-phase recipient cells carrying surface exclusion-deficient Flac mutants indicated that F does not itself determine a generalized recombination system able to replace the RecA plus product or the RecBC and RecF pathways."}
{"id": "PMID:1090576", "title": "Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.", "content": "Colicin plasmids E2 and E3 (Col E2 and Col E3) deoxyribonucleic acid (DNA) has been shown to contain, respectively, two and three EcoR1 restriction endonuclease-sensitive sites. This was determined by measuring the DNA fragments generated after EcoR1 endonuclease treatment by agarose gel electrophoresis and electron microscopy. The structure of heteroduplex Col E2-col E3 DNA molecules formed from EcoR1-generated fragments permitted a localization of the EcoR1-sensitive sites on the plasmid chromosomes.", "contents": "Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites. Colicin plasmids E2 and E3 (Col E2 and Col E3) deoxyribonucleic acid (DNA) has been shown to contain, respectively, two and three EcoR1 restriction endonuclease-sensitive sites. This was determined by measuring the DNA fragments generated after EcoR1 endonuclease treatment by agarose gel electrophoresis and electron microscopy. The structure of heteroduplex Col E2-col E3 DNA molecules formed from EcoR1-generated fragments permitted a localization of the EcoR1-sensitive sites on the plasmid chromosomes."}
{"id": "PMID:1090577", "title": "Rejoining of radiation-induced single-strand breaks in deoxyribonucleic acid of Escherichia coli: effect of phenethyl alcohol.", "content": "Single-strand breaks in deoxyribonucleic acid of Escherichia coli B/r cells exposed to 20 krads of gamma radiation could be rejoined by incubation of irradiated cells in growth medium. In the presence of 0.25% phenethyl alcohol, this repair was completely inhibited although deoxyribonucleic acid and protein syntheses were suppressed only partially.", "contents": "Rejoining of radiation-induced single-strand breaks in deoxyribonucleic acid of Escherichia coli: effect of phenethyl alcohol. Single-strand breaks in deoxyribonucleic acid of Escherichia coli B/r cells exposed to 20 krads of gamma radiation could be rejoined by incubation of irradiated cells in growth medium. In the presence of 0.25% phenethyl alcohol, this repair was completely inhibited although deoxyribonucleic acid and protein syntheses were suppressed only partially."}
{"id": "PMID:1090578", "title": "Lipid synthesis in stringent Escherichia coli: an artifact in acetate labeling of phospholipids during a shiftdown in growth rate.", "content": "The use of (14C)acetate to label the phospholipids of stringent Escherichia coli, after a decrease in agitation, leads to artifacts resulting from a decrease in the specific activity of the acetyl coenzyme A pool.", "contents": "Lipid synthesis in stringent Escherichia coli: an artifact in acetate labeling of phospholipids during a shiftdown in growth rate. The use of (14C)acetate to label the phospholipids of stringent Escherichia coli, after a decrease in agitation, leads to artifacts resulting from a decrease in the specific activity of the acetyl coenzyme A pool."}
{"id": "PMID:1090579", "title": "Genetic modification of substrate specificity of hypoxanthine phosphoribosyltransferase in Salmonella typhimurium.", "content": "Salmonella typhimurium strain GP660 (proAB-gpt deletion, purE) lacks guanine phosphoribosyltransferase and hence cannot utilize guanine as a purine source and is resistant to inhibition by 8-azaguanine. Strain GP660 was mutagenized and a derivative strain (GP36) was isolated for utilization of guanine and hypoxanthine, but not xanthine, as purine sources. This alteration was designated sug. The strain was then sensitive to inhibition by 8-azaguanine. Column chromatographic analysis revealed the altered phosphoribosyltransferase peaks for both hypoxanthine and guanine to be located together, in the same position as hypoxanthine phosphoribosyltransferase (hpt gene product) of the wild-type strain. Genetic analysis showed the sug mutation to be allelic with hpt. Therefore sug represented a modification of the substrate specificity of the hpt gene product.", "contents": "Genetic modification of substrate specificity of hypoxanthine phosphoribosyltransferase in Salmonella typhimurium. Salmonella typhimurium strain GP660 (proAB-gpt deletion, purE) lacks guanine phosphoribosyltransferase and hence cannot utilize guanine as a purine source and is resistant to inhibition by 8-azaguanine. Strain GP660 was mutagenized and a derivative strain (GP36) was isolated for utilization of guanine and hypoxanthine, but not xanthine, as purine sources. This alteration was designated sug. The strain was then sensitive to inhibition by 8-azaguanine. Column chromatographic analysis revealed the altered phosphoribosyltransferase peaks for both hypoxanthine and guanine to be located together, in the same position as hypoxanthine phosphoribosyltransferase (hpt gene product) of the wild-type strain. Genetic analysis showed the sug mutation to be allelic with hpt. Therefore sug represented a modification of the substrate specificity of the hpt gene product."}
{"id": "PMID:1090580", "title": "Characteristics of cold-sensitive mutants of Escherichia coli K-12 defective in deoxyribonucleic acid replication.", "content": "Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with toluene. All four mutations are located at or near the dnaA locus on the E. coli genetic map. They are recessive to the wild-type allele and two of them can be integratively suppressed by F episomes.", "contents": "Characteristics of cold-sensitive mutants of Escherichia coli K-12 defective in deoxyribonucleic acid replication. Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with toluene. All four mutations are located at or near the dnaA locus on the E. coli genetic map. They are recessive to the wild-type allele and two of them can be integratively suppressed by F episomes."}
{"id": "PMID:1090581", "title": "Sedimentation analysis of deoxyribonucleic acid from thymine-starved Escherichia coli.", "content": "During thymine starvation, strand breaks accumulate in the chromosomal deoxyribonucleic acid (DNA) of Escherichia coli. This effect occurs to a varying extent in different strains and is particularly enhanced in strains deficient in DNA polymerase I. The inhibition of ribonucleic acid or protein synthesis suppresses the accumulation of strand breaks. In a polA strain, rifampin is more effective than chloramphenicol or puromycin in suppressing strand break accumulation. To a certain extent the pehenomenon othymineless death correlates with the appearance of strand breaks. Although the killing can not be explained by the bulk of strand breaks, it is possible that some of them represent lethal events. On the basis of our observations we proposed the following model. (i) Transcription may be accompanied by single-strand breaks in DNA. (ii) DNA polymerase I is involved in the efficient repair of these breaks. (iii) Thymine deprivation results in the accumulation of unrepaired breaks. (iv) Polymerase I-mediated repair is less affected by thymine deprivation than are the alternative pathways because it closes the breaks with short patches, requiring less thymine.", "contents": "Sedimentation analysis of deoxyribonucleic acid from thymine-starved Escherichia coli. During thymine starvation, strand breaks accumulate in the chromosomal deoxyribonucleic acid (DNA) of Escherichia coli. This effect occurs to a varying extent in different strains and is particularly enhanced in strains deficient in DNA polymerase I. The inhibition of ribonucleic acid or protein synthesis suppresses the accumulation of strand breaks. In a polA strain, rifampin is more effective than chloramphenicol or puromycin in suppressing strand break accumulation. To a certain extent the pehenomenon othymineless death correlates with the appearance of strand breaks. Although the killing can not be explained by the bulk of strand breaks, it is possible that some of them represent lethal events. On the basis of our observations we proposed the following model. (i) Transcription may be accompanied by single-strand breaks in DNA. (ii) DNA polymerase I is involved in the efficient repair of these breaks. (iii) Thymine deprivation results in the accumulation of unrepaired breaks. (iv) Polymerase I-mediated repair is less affected by thymine deprivation than are the alternative pathways because it closes the breaks with short patches, requiring less thymine."}
{"id": "PMID:1090582", "title": "F deoxyribonucleic acid transferred to recipient cells in the presence of rifampin.", "content": "When Escherichia coli F+ cells resistant to rifampin and streptomycin are mated with F- cells sensitive to the antibiotics, a large fraction of F factor deoxyribonucleic acid (DNA), which is transferred to the recipient cells in the presence of either of the antibiotics, can be recovered as covalently closed, circular double-stranded DNA, as in the control mating in the absence of antibiotic. Similar results were obtained in the presence of chloramphenicol or chloramphenicol plus rifampin. It is suggested that the transferred single-stranded F DNA can be converted to the covalently closed, circular double-stranded form in the absence of protein synthesis, and that rifampin-sensitive transcription is not required for the conversion.", "contents": "F deoxyribonucleic acid transferred to recipient cells in the presence of rifampin. When Escherichia coli F+ cells resistant to rifampin and streptomycin are mated with F- cells sensitive to the antibiotics, a large fraction of F factor deoxyribonucleic acid (DNA), which is transferred to the recipient cells in the presence of either of the antibiotics, can be recovered as covalently closed, circular double-stranded DNA, as in the control mating in the absence of antibiotic. Similar results were obtained in the presence of chloramphenicol or chloramphenicol plus rifampin. It is suggested that the transferred single-stranded F DNA can be converted to the covalently closed, circular double-stranded form in the absence of protein synthesis, and that rifampin-sensitive transcription is not required for the conversion."}
{"id": "PMID:1090583", "title": "F deoxyribonucleic acid superinfected into phenocopies of donor strains.", "content": "When F+ donor cells of Escherichia coli are conjugated with F-, F+, or Hfr recipients under the conditions of phenocopy mating, the male recipients are found capable of accepting the F episome as effectively as the F- recipients. The F deoxyribonucleic acid (DNA) superinfected into the male recipients is converted to the covalently closed, circular duplex form, as in the F- recipients. It is also found that the synthesis of the strand complementary to the transferred single strand and its subsequent conversion to the covalently closed, circular duplex occur effectively in male recipients as well as in female recipients. Under these mating conditions, F-ilv+ episome superinfected to F+ and Hfr cells is diluted out during growth, whereas F-ilv+ transferred into F-cells is replicated and established in almost all progeny cells. These results suggest that the incompatibility of the F episome is not due to the reduction in the rate of the conversion of transferred single-straned F DNA to covalently closed, circular duplex, but, rather, to an inhibition of further replication of the covalently closed, circular F DNA.", "contents": "F deoxyribonucleic acid superinfected into phenocopies of donor strains. When F+ donor cells of Escherichia coli are conjugated with F-, F+, or Hfr recipients under the conditions of phenocopy mating, the male recipients are found capable of accepting the F episome as effectively as the F- recipients. The F deoxyribonucleic acid (DNA) superinfected into the male recipients is converted to the covalently closed, circular duplex form, as in the F- recipients. It is also found that the synthesis of the strand complementary to the transferred single strand and its subsequent conversion to the covalently closed, circular duplex occur effectively in male recipients as well as in female recipients. Under these mating conditions, F-ilv+ episome superinfected to F+ and Hfr cells is diluted out during growth, whereas F-ilv+ transferred into F-cells is replicated and established in almost all progeny cells. These results suggest that the incompatibility of the F episome is not due to the reduction in the rate of the conversion of transferred single-straned F DNA to covalently closed, circular duplex, but, rather, to an inhibition of further replication of the covalently closed, circular F DNA."}
{"id": "PMID:1090584", "title": "Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli.", "content": "fabD mutants of Escherichia coli contain a thermolabile malonyl-coenzyme A-acyl carrier protein transacylase which causes defective fatty acid synthesis and temperature-sensitive growth. By conjugation and P1 transduction the fabD locus has now been mapped at min 24, between pyrC and purB and close to cat. The order of sites is tentatively given as pyrC, cat, fabD, and purB, though the orientation of cat and fabD could be reversed. The possible relationship of fabD with another mutation lying in this region and also affecting acid synthesis is discussed. In the course of these studies we also confirmed the location of the fabA gene, determined that poaA lies between fabA and pyrC, and inadvertently found that the pyr mutation in strain AT3143 is probably pyrF and not pyrC.", "contents": "Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli. fabD mutants of Escherichia coli contain a thermolabile malonyl-coenzyme A-acyl carrier protein transacylase which causes defective fatty acid synthesis and temperature-sensitive growth. By conjugation and P1 transduction the fabD locus has now been mapped at min 24, between pyrC and purB and close to cat. The order of sites is tentatively given as pyrC, cat, fabD, and purB, though the orientation of cat and fabD could be reversed. The possible relationship of fabD with another mutation lying in this region and also affecting acid synthesis is discussed. In the course of these studies we also confirmed the location of the fabA gene, determined that poaA lies between fabA and pyrC, and inadvertently found that the pyr mutation in strain AT3143 is probably pyrF and not pyrC."}
{"id": "PMID:1090585", "title": "Vinylglycolate resistance in Escherichia coli.", "content": "Escherichia coli K-12 vinylglycolate-resistant mutants have been isolated and characterized. Two of the mutants, JSH 150 and JSH 151, have been determined to be double mutants, lacking both membrane-bound L-and D-lactate dehydrogenases. The lactate transport system is intact in all strains; both radioactive lactate and vinylglycolate are actively taken up. Likewise, the phosphoenolypyruvate-dependent phosphotransferase system for hexose uptake is active. Vinylglycolate, previously shown to inhibit the phosphoenolpyruvate-dependent phosphotransferase system, has very little effect in the double mutants. The extent of vinylglycolate inhibition in other mutants seems directly related to the activity of the lactate dehydrogenases. This indicates that vinylglycolate is oxidized to 2-keto-3-butenoate before inactivating the phosphoenolpyruvate-dependent phosphotransferase system. These results were found in whole cells and confirmed in isolated membrane vesicles.", "contents": "Vinylglycolate resistance in Escherichia coli. Escherichia coli K-12 vinylglycolate-resistant mutants have been isolated and characterized. Two of the mutants, JSH 150 and JSH 151, have been determined to be double mutants, lacking both membrane-bound L-and D-lactate dehydrogenases. The lactate transport system is intact in all strains; both radioactive lactate and vinylglycolate are actively taken up. Likewise, the phosphoenolypyruvate-dependent phosphotransferase system for hexose uptake is active. Vinylglycolate, previously shown to inhibit the phosphoenolpyruvate-dependent phosphotransferase system, has very little effect in the double mutants. The extent of vinylglycolate inhibition in other mutants seems directly related to the activity of the lactate dehydrogenases. This indicates that vinylglycolate is oxidized to 2-keto-3-butenoate before inactivating the phosphoenolpyruvate-dependent phosphotransferase system. These results were found in whole cells and confirmed in isolated membrane vesicles."}
{"id": "PMID:1090586", "title": "Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae.", "content": "Our previous work has shown that both induction, after addition of inducer, and loss of ability to produce allophanate hydrolase, after removal of inducer, proceed more rapidly than expected from the reported half-life of messenger ribonucleic acid in Saccharomyces cerevisiae. As a basis of rectifying these observations, we have characterized induction and repression of allophanate hydrolase synthesis and find that: (i) induction of the hydrolase begins immediately upon addition of inducer, (ii) once induction has been initiated removal of inducer does not result in immediate loss of synthetic capacity, (iii) induction of the capacity to produce hydrolase can occur in the absence of protein synthesis, (iv) the half-life of hydrolase synthetic capacity increases if protein synthesis is inhibited, (v) allophanate hydrolase itself is not degraded upon removal of inducer, and (vi) induction and repression of allophanate hydrolase synthetic capacity likely occurs at the level of transcription.", "contents": "Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae. Our previous work has shown that both induction, after addition of inducer, and loss of ability to produce allophanate hydrolase, after removal of inducer, proceed more rapidly than expected from the reported half-life of messenger ribonucleic acid in Saccharomyces cerevisiae. As a basis of rectifying these observations, we have characterized induction and repression of allophanate hydrolase synthesis and find that: (i) induction of the hydrolase begins immediately upon addition of inducer, (ii) once induction has been initiated removal of inducer does not result in immediate loss of synthetic capacity, (iii) induction of the capacity to produce hydrolase can occur in the absence of protein synthesis, (iv) the half-life of hydrolase synthetic capacity increases if protein synthesis is inhibited, (v) allophanate hydrolase itself is not degraded upon removal of inducer, and (vi) induction and repression of allophanate hydrolase synthetic capacity likely occurs at the level of transcription."}
{"id": "PMID:1090587", "title": "Escherichia coli K-12 mutant forming a temperature-sensitive D-serine deaminase.", "content": "A single-site mutant of Escherichia coli K-12 able to grow in minimal medium in the presence of D-serine at 30 C but not at 42 C was isolated. The mutant forms a D-serine deaminase that is much more sensitive to thermal denaturation in vitro at temperatures above but not below 47 C than that of the wild type. No detectable enzyme is formed by the mutant at 42 C, however, and very little is formed at 37 C. The mutant enzyme is probably more sensitive to intracellular inactivation at high temperatures than the wild-type enzyme. The mutation lies in the dsdA region. The mutant also contains a dsdO mutation, which does not permit hyperinduction of D-serine deaminase synthesis.", "contents": "Escherichia coli K-12 mutant forming a temperature-sensitive D-serine deaminase. A single-site mutant of Escherichia coli K-12 able to grow in minimal medium in the presence of D-serine at 30 C but not at 42 C was isolated. The mutant forms a D-serine deaminase that is much more sensitive to thermal denaturation in vitro at temperatures above but not below 47 C than that of the wild type. No detectable enzyme is formed by the mutant at 42 C, however, and very little is formed at 37 C. The mutant enzyme is probably more sensitive to intracellular inactivation at high temperatures than the wild-type enzyme. The mutation lies in the dsdA region. The mutant also contains a dsdO mutation, which does not permit hyperinduction of D-serine deaminase synthesis."}
{"id": "PMID:1090588", "title": "Isolation and characterization of D-serine deaminase constitutive mutants by utilization of D-serine as sole carbon or nitrogen source.", "content": "Mutants constitutive for D-serine deaminase (Dsdase) synthesis were isolated by utilizing D-serine as sole nitrogen or carbon source in the chemostat. This method generated only regulatory constitutive (dsdC) mutants. The altered dsdC gene product in these strains is apparently able to bind D-serine more efficiently than the wild-type dsdC+ gene product--a selective advantage. Constitutive synthesis of Dsdase in all of these dsdC mutants is extremely sensitive to catabolite repression, and catabolite repression is reversed by the addition of D-serine. Of the 15 mutants generated by this method, none are suppressible by supD, supE, or supF. Mutations to a low level of constitutivity (maximal specific activity of 9) occur much more frequently than mutations to a high level (maximal specific activity of 79). High level constitutive synthesis of Dsdase results from the synthesis of an altered dsdC gene product--not from loss of ability to form the dsdC product. Dsdase synthesis is not regulated by the nitrogen supply in the medium, as nitrogen starvation does not result in the derepression of Dsdase synthesis.", "contents": "Isolation and characterization of D-serine deaminase constitutive mutants by utilization of D-serine as sole carbon or nitrogen source. Mutants constitutive for D-serine deaminase (Dsdase) synthesis were isolated by utilizing D-serine as sole nitrogen or carbon source in the chemostat. This method generated only regulatory constitutive (dsdC) mutants. The altered dsdC gene product in these strains is apparently able to bind D-serine more efficiently than the wild-type dsdC+ gene product--a selective advantage. Constitutive synthesis of Dsdase in all of these dsdC mutants is extremely sensitive to catabolite repression, and catabolite repression is reversed by the addition of D-serine. Of the 15 mutants generated by this method, none are suppressible by supD, supE, or supF. Mutations to a low level of constitutivity (maximal specific activity of 9) occur much more frequently than mutations to a high level (maximal specific activity of 79). High level constitutive synthesis of Dsdase results from the synthesis of an altered dsdC gene product--not from loss of ability to form the dsdC product. Dsdase synthesis is not regulated by the nitrogen supply in the medium, as nitrogen starvation does not result in the derepression of Dsdase synthesis."}
{"id": "PMID:1090589", "title": "Positive control in the D-serine deaminase system of Escherichia coli K-12.", "content": "Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator negative) mutations. The mutations lie in the dsdC region, are cis and trans recessive to dsdC+, and give rise to revertants of novel regulatory phenotype. The second class consists of Dsdase-negative lysogens in which the phenotype is the result of the integration of lambdac1857 Sam7 into the dsdC region. Lambda lysates derived from two of the Dsdase-negative lysogens can transduce the structural gene for Dsdase (dsdA) but not the dsdC region. The dsdC+ gene product had no repressor effect on constitutive synthesis in a strain containing a dsdO (initiator constitutive) and a dsdC- mutation. These and other findings indicate that control of Dsdase synthesis is strictly positive. The partial trans effect of the dsdC+ gene product on constitutive synthesis in dsdCc (regulator constitutive) strains can thus be explained by \"subunit mixing\" between active dsdCc subunits and dsdC+ subunits which are inactive in the absence of the inducer, D-serine. The order of genes in the dsd region is supN-dsdC-dsdP-dsdA-aroC.", "contents": "Positive control in the D-serine deaminase system of Escherichia coli K-12. Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator negative) mutations. The mutations lie in the dsdC region, are cis and trans recessive to dsdC+, and give rise to revertants of novel regulatory phenotype. The second class consists of Dsdase-negative lysogens in which the phenotype is the result of the integration of lambdac1857 Sam7 into the dsdC region. Lambda lysates derived from two of the Dsdase-negative lysogens can transduce the structural gene for Dsdase (dsdA) but not the dsdC region. The dsdC+ gene product had no repressor effect on constitutive synthesis in a strain containing a dsdO (initiator constitutive) and a dsdC- mutation. These and other findings indicate that control of Dsdase synthesis is strictly positive. The partial trans effect of the dsdC+ gene product on constitutive synthesis in dsdCc (regulator constitutive) strains can thus be explained by \"subunit mixing\" between active dsdCc subunits and dsdC+ subunits which are inactive in the absence of the inducer, D-serine. The order of genes in the dsd region is supN-dsdC-dsdP-dsdA-aroC."}
{"id": "PMID:1090590", "title": "Solubilization of Escherichia coli nitrate reductase by a membrane-bound protease.", "content": "Nitrate reductase extracted from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit not present in the purified enzyme used to prepare the antibody. Nitrate reductase precipitated by antibody from alkaline heat extracts was composed of peptide fragments of various sizes. These fragments were produced by a membrane-bound protease which was activated by alkaline pH and heat. It is the action of this protease that releases the enzyme from the membrane, as shown by the observations that protease inhibitors decreased the amount of solubilization of the enzyme, and the enzyme remaining in the membrane after heating showed much less proteolytic cleavage than that which was released.", "contents": "Solubilization of Escherichia coli nitrate reductase by a membrane-bound protease. Nitrate reductase extracted from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit not present in the purified enzyme used to prepare the antibody. Nitrate reductase precipitated by antibody from alkaline heat extracts was composed of peptide fragments of various sizes. These fragments were produced by a membrane-bound protease which was activated by alkaline pH and heat. It is the action of this protease that releases the enzyme from the membrane, as shown by the observations that protease inhibitors decreased the amount of solubilization of the enzyme, and the enzyme remaining in the membrane after heating showed much less proteolytic cleavage than that which was released."}
{"id": "PMID:1090591", "title": "Anaerobic cytochrome b1 in Escherichia coli: association with and regulation of nitrate reductase.", "content": "Nitrate reductase solubilized from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit, not present in the purified enzyme used to prepare the antibody. This third subunit was identified as the cytochrome b1 apoprotein. This cytochrome is bound to nitrate reductase from wild-type E. coli in a ratio of 2 mol of cytochrome per mol of enzyme complex. In mutants unable to synthesize heme, this cytochrome b1 apoprotein is not bound to nitrate reductase. In these same mutants, the enzyme is overproduced and accumulates in the cytoplasm. The absence of cytochrome also affects the stability of the membrane-bound form of the enzyme.", "contents": "Anaerobic cytochrome b1 in Escherichia coli: association with and regulation of nitrate reductase. Nitrate reductase solubilized from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit, not present in the purified enzyme used to prepare the antibody. This third subunit was identified as the cytochrome b1 apoprotein. This cytochrome is bound to nitrate reductase from wild-type E. coli in a ratio of 2 mol of cytochrome per mol of enzyme complex. In mutants unable to synthesize heme, this cytochrome b1 apoprotein is not bound to nitrate reductase. In these same mutants, the enzyme is overproduced and accumulates in the cytoplasm. The absence of cytochrome also affects the stability of the membrane-bound form of the enzyme."}
{"id": "PMID:1090592", "title": "Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli.", "content": "Specific antibody to purified nitrate reductase from Escherichia coli was used to identify enzyme components present in mutants which lack functional nitrate reductase. chlA and B mutants contained all three subunits present in the wild-type enzyme. Different peptides with a broad range of molecular weights could be precipitated from chlCmutants, and chlE mutants contained either slightly degraded enzyme subunits or no precipitable protein. No mutants produced significant amounts of cytoplasmic enzyme. The chlA and B loci are suggested to function in the synthesis and attachment of a molybdenum-containing factor. The chlC locus is suggested to be the structural gene for nitrate reductase subunit A and chlE is suggested to be involved in the synthesis of the cytochrome b1 apoprotein.", "contents": "Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli. Specific antibody to purified nitrate reductase from Escherichia coli was used to identify enzyme components present in mutants which lack functional nitrate reductase. chlA and B mutants contained all three subunits present in the wild-type enzyme. Different peptides with a broad range of molecular weights could be precipitated from chlCmutants, and chlE mutants contained either slightly degraded enzyme subunits or no precipitable protein. No mutants produced significant amounts of cytoplasmic enzyme. The chlA and B loci are suggested to function in the synthesis and attachment of a molybdenum-containing factor. The chlC locus is suggested to be the structural gene for nitrate reductase subunit A and chlE is suggested to be involved in the synthesis of the cytochrome b1 apoprotein."}
{"id": "PMID:1090593", "title": "Effects of methotrexate on intraperiplasmic and axenic growth of Bdellovibrio bacteriovorus.", "content": "The intraperiplasmic growth rate and cell yield of wild-type Bdellovibrio bacteriovorus 109J, growing on Escherichia coli of normal composition as the substrate, were not markedly inhibited by 10-3 M methotrexate (4-amino-N10-methylpteroylglutamic acid). In contrast, the growth rate and cell yield of the mutant 109Ja, growing axenically in 0.5% yeast extract +0.15% peptone, were strongly inhibited by 10-4 and 10-3 M methotrexate. Thymine, thymidine, and thymidine-5'-monophosphate, in increasing order of effectiveness, partially or completely reversed the inhibition. E. coli depleted of tetrahydrofolate and having an abnormally high protein/deoxyribonucleic acid (DNA) ratio was obtained by growing it in the presence of methotrexate. B. bacteriovourus grew at a normal rate on these depleted E. coli cells but with somewhat reduced cell yield. Mexthotrexate (10-3 M) inhibited intraperiplasmic growth of bdellovibrio on the depleted E. coli somewhat more than it inhibited growth on normal E. coli, but the effects were small compared with inhibition of axenic growth of the  mutant. Total bdellovibrio DNA after growth on the depleted E. coli in the presence or absence of methotrexate exceeded the initial quanity of E. coli DNA present. Thymidine-5'-monophosphate (10-3 M) largely reversed the inhibition and increased the amount of net synthesis of DNA. The data are consistent with the prediction that intraperiplasmic growth of B. bacteriovorus should be insensitive to all metabolic inhibitors that act by specifically preventing synthesis of essential monomers. The data also indicate that B. bacteriovorus possesses thymidylate synthetase, thymidine phosphorylase, and thymidine kinase, and has the potential to carry out de novo DNA synthesis from non-DNA precursors during intraperiplasmic growth. The results also suggest that methionyl tRNAfMet is not required for initiation of protein synthesis by B. bacteriovorus.", "contents": "Effects of methotrexate on intraperiplasmic and axenic growth of Bdellovibrio bacteriovorus. The intraperiplasmic growth rate and cell yield of wild-type Bdellovibrio bacteriovorus 109J, growing on Escherichia coli of normal composition as the substrate, were not markedly inhibited by 10-3 M methotrexate (4-amino-N10-methylpteroylglutamic acid). In contrast, the growth rate and cell yield of the mutant 109Ja, growing axenically in 0.5% yeast extract +0.15% peptone, were strongly inhibited by 10-4 and 10-3 M methotrexate. Thymine, thymidine, and thymidine-5'-monophosphate, in increasing order of effectiveness, partially or completely reversed the inhibition. E. coli depleted of tetrahydrofolate and having an abnormally high protein/deoxyribonucleic acid (DNA) ratio was obtained by growing it in the presence of methotrexate. B. bacteriovourus grew at a normal rate on these depleted E. coli cells but with somewhat reduced cell yield. Mexthotrexate (10-3 M) inhibited intraperiplasmic growth of bdellovibrio on the depleted E. coli somewhat more than it inhibited growth on normal E. coli, but the effects were small compared with inhibition of axenic growth of the  mutant. Total bdellovibrio DNA after growth on the depleted E. coli in the presence or absence of methotrexate exceeded the initial quanity of E. coli DNA present. Thymidine-5'-monophosphate (10-3 M) largely reversed the inhibition and increased the amount of net synthesis of DNA. The data are consistent with the prediction that intraperiplasmic growth of B. bacteriovorus should be insensitive to all metabolic inhibitors that act by specifically preventing synthesis of essential monomers. The data also indicate that B. bacteriovorus possesses thymidylate synthetase, thymidine phosphorylase, and thymidine kinase, and has the potential to carry out de novo DNA synthesis from non-DNA precursors during intraperiplasmic growth. The results also suggest that methionyl tRNAfMet is not required for initiation of protein synthesis by B. bacteriovorus."}
{"id": "PMID:1090594", "title": "Utilization of nucleoside monophosphates per Se for intraperiplasmic growth of Bdellovibrio bacteriovorus.", "content": "During growth of Bdellovibrio bacteriovorus on Escherichia coli, there was a marked preferential use of E. coli phosphorus over exogenous orthophosphate even though the latter permeated into the intraperiplasmic space where the bdellovibrio was growing. This preferential use occurred to an equal extent for lipid phosphorus and nucleic acid phosphorus. Exogenous thymidine-5'-monophosphate competed effectively with [3H]thymine residues of E. coli as a precursor for bdellovibrio deoxyribonucleic acid; exogenous thymidine competed less effectively and thymine and uridine not at all. A mixture of exogenous nucleoside-5'-monophosphates equilibrated effectively with E. coli phosphorus as a phosphorus source for B. bacteriovorus; the nucleotide phosphorus entered preferentially into bdellovibrio nucleic acids. A comparable mixture of exogenous nucleosides plus orthophosphate had only a small effect on utilization of E. coli phosphorus by B. bacteriovorus, as did orthophosphate alone. A mixture of exogenous deoxyriboside monophosphates equilibrium effectively with E. coli phosphorus as a phosphorus source for bdellovibrio growth; the phosphorus from this source entered preferentially into deoxyribonucleic acid. These data show that nucleoside monophosphates derived from the substrate organism are utilized directly for n-cleic acid biosynthesis by B. bacteriovorus growing intraperiplasmically. As a consequence, the phosphate ester bonds preexisting in the nucleic acids of the substrate organism are conserved by the bdellovibrio, presumably lessening its energy requirement for intraperiplasmic growth. The data also suggest, but do not prove, that the phosphate ester bonds of phospholipids are also conserved.", "contents": "Utilization of nucleoside monophosphates per Se for intraperiplasmic growth of Bdellovibrio bacteriovorus. During growth of Bdellovibrio bacteriovorus on Escherichia coli, there was a marked preferential use of E. coli phosphorus over exogenous orthophosphate even though the latter permeated into the intraperiplasmic space where the bdellovibrio was growing. This preferential use occurred to an equal extent for lipid phosphorus and nucleic acid phosphorus. Exogenous thymidine-5'-monophosphate competed effectively with [3H]thymine residues of E. coli as a precursor for bdellovibrio deoxyribonucleic acid; exogenous thymidine competed less effectively and thymine and uridine not at all. A mixture of exogenous nucleoside-5'-monophosphates equilibrated effectively with E. coli phosphorus as a phosphorus source for B. bacteriovorus; the nucleotide phosphorus entered preferentially into bdellovibrio nucleic acids. A comparable mixture of exogenous nucleosides plus orthophosphate had only a small effect on utilization of E. coli phosphorus by B. bacteriovorus, as did orthophosphate alone. A mixture of exogenous deoxyriboside monophosphates equilibrium effectively with E. coli phosphorus as a phosphorus source for bdellovibrio growth; the phosphorus from this source entered preferentially into deoxyribonucleic acid. These data show that nucleoside monophosphates derived from the substrate organism are utilized directly for n-cleic acid biosynthesis by B. bacteriovorus growing intraperiplasmically. As a consequence, the phosphate ester bonds preexisting in the nucleic acids of the substrate organism are conserved by the bdellovibrio, presumably lessening its energy requirement for intraperiplasmic growth. The data also suggest, but do not prove, that the phosphate ester bonds of phospholipids are also conserved."}
{"id": "PMID:1090595", "title": "Incorporation of long-chain fatty acids of the substrate organism by Bdellovibrio bacteriovorus during intraperiplasmic growth.", "content": "Data are presented showing that a large proportion of the fatty acids of Bdellovibrio bacteriovorus grown intraperiplasmically are derived unaltered from the fatty acids of its substrate organism. Those fatty acids of the bdellovibrio not homologous with those of the substrate organism are derived mainly by metabolic alteration of preexisting fatty acids in the latter. De novo synthesis from acetate occurs only to a small extent. These characteristics of bdellovibrio physiology are in part responsible for its minimal energy expenditure for intraperiplasmic growth. The data presented also indicate that B. bacteriovorus is capable of hydrogenating unsaturated fatty acids, of beta-oxidation of fatty acids, and of regulating the proportion of saturated and unsaturated fatty acids in the lipids.", "contents": "Incorporation of long-chain fatty acids of the substrate organism by Bdellovibrio bacteriovorus during intraperiplasmic growth. Data are presented showing that a large proportion of the fatty acids of Bdellovibrio bacteriovorus grown intraperiplasmically are derived unaltered from the fatty acids of its substrate organism. Those fatty acids of the bdellovibrio not homologous with those of the substrate organism are derived mainly by metabolic alteration of preexisting fatty acids in the latter. De novo synthesis from acetate occurs only to a small extent. These characteristics of bdellovibrio physiology are in part responsible for its minimal energy expenditure for intraperiplasmic growth. The data presented also indicate that B. bacteriovorus is capable of hydrogenating unsaturated fatty acids, of beta-oxidation of fatty acids, and of regulating the proportion of saturated and unsaturated fatty acids in the lipids."}
{"id": "PMID:1090596", "title": "Energy efficiency of intraperiplasmic growth of Bdellovibrio bacteriovorus.", "content": "The Y-ATP (energy efficiency) of intraperiplasmic growth of Bdellovibrio bacteriovorus was determined from the distribution of radioactivity of the substrate organism ([U-14C]Escherichia coli) btween CO2 and bdellovibrio cells at the end of growth. A \"best\" Y-ATP value of 18.5 was obtained from single growth cycle experiments and an average value of 25.9 from multicycle experiments. Both values are much higher than the usual value of 10.5 for bacteria growing in rich media. The bases for the unusual energy efficiency for growth of B. bacteriovorus are discussed.", "contents": "Energy efficiency of intraperiplasmic growth of Bdellovibrio bacteriovorus. The Y-ATP (energy efficiency) of intraperiplasmic growth of Bdellovibrio bacteriovorus was determined from the distribution of radioactivity of the substrate organism ([U-14C]Escherichia coli) btween CO2 and bdellovibrio cells at the end of growth. A \"best\" Y-ATP value of 18.5 was obtained from single growth cycle experiments and an average value of 25.9 from multicycle experiments. Both values are much higher than the usual value of 10.5 for bacteria growing in rich media. The bases for the unusual energy efficiency for growth of B. bacteriovorus are discussed."}
{"id": "PMID:1090597", "title": "Molecular mechanisms of accommodation in Escherichia coli to toxic levels of Cd2+.", "content": "Cells of Escherichia coli strain B develop large intracellular vacuoles and exhibit an abnormally long lag phase when inoculated into a defined medium to glucose and salts containing 3 times 10-6 M Cd2+. Early in this lag, about 95% of the cells fail to form colonies when plated on nutrient broth-NaCl-agar. Prior to the initiation of proliferation, the morphology of these cells becomes normal. They regain viability in the absence of deoxyribonucleic acid replication. The rate and extent of growth are normal once proliferation begins. This reversible phenomenon of accommodation to a growth-inhibiting concentration of Cd2+ does not appear to result from a selection of mutant cells. Cells which are proliferating in the presence of Cd2+ accumulate the ion to a very high concentration. In membranes and 31% in the cytoplasm. In unaccommodated cells, the figures are 2%, 75%, and 23%, respectively. The activity of alkaline phosphatase, a zinc-metalloenzyme which is inhibited by cadmum and is located between cell wall and membrane, is not abnormally low in accommodated cells, suggesting that the cadmim is compartmentalized in these cells. Molecular sieve chromatography of cell extracts shows that the Cd2+ is associated with two classes of macromolecules. It appears that accommodation of E. coli to the presence of Cd2+ involves exclusion of the ion from the cell and reversal of damage caused by prior exposure to the ion.", "contents": "Molecular mechanisms of accommodation in Escherichia coli to toxic levels of Cd2+. Cells of Escherichia coli strain B develop large intracellular vacuoles and exhibit an abnormally long lag phase when inoculated into a defined medium to glucose and salts containing 3 times 10-6 M Cd2+. Early in this lag, about 95% of the cells fail to form colonies when plated on nutrient broth-NaCl-agar. Prior to the initiation of proliferation, the morphology of these cells becomes normal. They regain viability in the absence of deoxyribonucleic acid replication. The rate and extent of growth are normal once proliferation begins. This reversible phenomenon of accommodation to a growth-inhibiting concentration of Cd2+ does not appear to result from a selection of mutant cells. Cells which are proliferating in the presence of Cd2+ accumulate the ion to a very high concentration. In membranes and 31% in the cytoplasm. In unaccommodated cells, the figures are 2%, 75%, and 23%, respectively. The activity of alkaline phosphatase, a zinc-metalloenzyme which is inhibited by cadmum and is located between cell wall and membrane, is not abnormally low in accommodated cells, suggesting that the cadmim is compartmentalized in these cells. Molecular sieve chromatography of cell extracts shows that the Cd2+ is associated with two classes of macromolecules. It appears that accommodation of E. coli to the presence of Cd2+ involves exclusion of the ion from the cell and reversal of damage caused by prior exposure to the ion."}
{"id": "PMID:1090598", "title": "Correlation between thymineless elimination and absence of hsp II (EcoRII) specificity in N-group R factors.", "content": "Examination of R factors from 12 different compatibility groups shows that thymineless elimination is apparently confined to N-group plasmids that lack the hsp II (EcoRII) restriction specificity.", "contents": "Correlation between thymineless elimination and absence of hsp II (EcoRII) specificity in N-group R factors. Examination of R factors from 12 different compatibility groups shows that thymineless elimination is apparently confined to N-group plasmids that lack the hsp II (EcoRII) restriction specificity."}
{"id": "PMID:1090599", "title": "Role of ribonucleic acid synthesis in replication of deoxyribonucleic acid.", "content": "An experiment previously interpreted to show a ribonucleic acid requirement for propagation of deoxyribonucleic replication is reexamined and the earlier interpretation is shown to be incorrect.", "contents": "Role of ribonucleic acid synthesis in replication of deoxyribonucleic acid. An experiment previously interpreted to show a ribonucleic acid requirement for propagation of deoxyribonucleic replication is reexamined and the earlier interpretation is shown to be incorrect."}
{"id": "PMID:1090600", "title": "Procedure for isolating mutants defective in metabolite transport or utilization.", "content": "Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite.", "contents": "Procedure for isolating mutants defective in metabolite transport or utilization. Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite."}
{"id": "PMID:1090601", "title": "Stability of Escherichia coli polysomes at high hydrostatic pressure.", "content": "The stability of Escherichia coli polysomes at increased hydrostatic pressure was investigated in actively growing cells, in which the initiation of transcription was blocked by rifampin. In these cells, [3-H]uridine incorporation into messenger ribonucleic acid and the subsequent degradation of the message (and therefore of polysomes) by ribonuclease could be observed. Evidence is presented that the activity of the RNases is unaffected by a pressure of 680 atm, that protein synthesis is completely inhibited at 680 atm but immediately resumes at the 1 atm rate on release of pressure, and that no degradation of messenger ribonucleic acid in polysomes occurs at 680 atm. The effects of pressure; puromycin, and chloramphenicol on polysomal degradation are discussed. These results indicate that, contrary to some previous reports, polysomes are probably stabilized by high pressures. Therefore, we consider that polysomal instability is not a factor in the inhibition of protein synthesis by high pressures.", "contents": "Stability of Escherichia coli polysomes at high hydrostatic pressure. The stability of Escherichia coli polysomes at increased hydrostatic pressure was investigated in actively growing cells, in which the initiation of transcription was blocked by rifampin. In these cells, [3-H]uridine incorporation into messenger ribonucleic acid and the subsequent degradation of the message (and therefore of polysomes) by ribonuclease could be observed. Evidence is presented that the activity of the RNases is unaffected by a pressure of 680 atm, that protein synthesis is completely inhibited at 680 atm but immediately resumes at the 1 atm rate on release of pressure, and that no degradation of messenger ribonucleic acid in polysomes occurs at 680 atm. The effects of pressure; puromycin, and chloramphenicol on polysomal degradation are discussed. These results indicate that, contrary to some previous reports, polysomes are probably stabilized by high pressures. Therefore, we consider that polysomal instability is not a factor in the inhibition of protein synthesis by high pressures."}
{"id": "PMID:1090602", "title": "Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.", "content": "Selected mutant strains of Klebsiella pneumoniae that are unable to fix nitrogen have been characterized according to nitrogenase component activity as well as antigenic cross-reacting material. The lesions in these strains have been mapped by transduction, and the results indicate that there are at least five genes specifically responsible for nitrogen fixation in vivo. Besides genes that specify the structure of the two nitrogenase components, there is a gene for a factor that is required for component I activity and a gene that codes for a factor possibly involved in electron transport to component II. A mutation in another site does not allow the organism to produce either of the nitrogenase components. All of these genes are co-transducible with the gene that specifics the structure of histidinol dehydrogenase.", "contents": "Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2. Selected mutant strains of Klebsiella pneumoniae that are unable to fix nitrogen have been characterized according to nitrogenase component activity as well as antigenic cross-reacting material. The lesions in these strains have been mapped by transduction, and the results indicate that there are at least five genes specifically responsible for nitrogen fixation in vivo. Besides genes that specify the structure of the two nitrogenase components, there is a gene for a factor that is required for component I activity and a gene that codes for a factor possibly involved in electron transport to component II. A mutation in another site does not allow the organism to produce either of the nitrogenase components. All of these genes are co-transducible with the gene that specifics the structure of histidinol dehydrogenase."}
{"id": "PMID:1090603", "title": "Regulation of branched-chain aminoacyl-transfer ribonucleic acid synthetases in an ilvDAC deletion strain of Escherichia coli K-12.", "content": "Valyl-, isoleucyl-, and leucyl-transfer ribonucleic acid synthetase formation was compared in isogenic strains of Escherichia coli K-12 that differed only in that one strain carried a deletion of three genes of the ilv gene cluster, ilvD, -A, and -C. It was found that: (i) the activities of these synthetases in the deletion strain were less than those in the normal strain during growth in minimal medium supplemented with excess isoleucine, valine, and leucine, and (ii) their stability was reduced in the deletion strain during specific branched-chain amino acid limitations. The results of density-labeling experiments suggest that the in vivo stability of valyl-, isoleucyl-, and leucyl-transfer ribonucleic acid synthetases requires some product missing in the ilvDAC deletion strain.", "contents": "Regulation of branched-chain aminoacyl-transfer ribonucleic acid synthetases in an ilvDAC deletion strain of Escherichia coli K-12. Valyl-, isoleucyl-, and leucyl-transfer ribonucleic acid synthetase formation was compared in isogenic strains of Escherichia coli K-12 that differed only in that one strain carried a deletion of three genes of the ilv gene cluster, ilvD, -A, and -C. It was found that: (i) the activities of these synthetases in the deletion strain were less than those in the normal strain during growth in minimal medium supplemented with excess isoleucine, valine, and leucine, and (ii) their stability was reduced in the deletion strain during specific branched-chain amino acid limitations. The results of density-labeling experiments suggest that the in vivo stability of valyl-, isoleucyl-, and leucyl-transfer ribonucleic acid synthetases requires some product missing in the ilvDAC deletion strain."}
{"id": "PMID:1090604", "title": "Integration of R plasmid Rts1 to the gal region of the Escherichia coli chromosome.", "content": "An R plasmid Rts1 was integrated into the gal region of the chromosome of Escherichia coli XA-7012 (galE) strain by the directed transposition technique. The integration of the Rts1 genome was confirmed mainly by conjugation studies and also by transduction experiments using phage P1. As a result, it was found that the integrated genome contained genes responsible for kanamycin resistance, conjugal transferability, and for autonomous replication. As reported previously, Rts1 is temperature sensitive in replication and inhibits the growth of the host at nonpermissive temperature. However, although a plasmid derived from the integrated Rts1 genome still demonstrates temperature sensitivity upon transfer and high level of kanamycin resistance, this plasmid no longer displays temperature sensitivity in replication and the inhibitory effect on the host. These results indicate that the temperature sensitivity of replication of Rts1 and its inhibitory effect on the host cell are due to the presence of a gene or gene cluster on the Rts1 genome and that the gene(s) is clearly discriminated from the one responsible for the temperature sensitivity of transfer.", "contents": "Integration of R plasmid Rts1 to the gal region of the Escherichia coli chromosome. An R plasmid Rts1 was integrated into the gal region of the chromosome of Escherichia coli XA-7012 (galE) strain by the directed transposition technique. The integration of the Rts1 genome was confirmed mainly by conjugation studies and also by transduction experiments using phage P1. As a result, it was found that the integrated genome contained genes responsible for kanamycin resistance, conjugal transferability, and for autonomous replication. As reported previously, Rts1 is temperature sensitive in replication and inhibits the growth of the host at nonpermissive temperature. However, although a plasmid derived from the integrated Rts1 genome still demonstrates temperature sensitivity upon transfer and high level of kanamycin resistance, this plasmid no longer displays temperature sensitivity in replication and the inhibitory effect on the host. These results indicate that the temperature sensitivity of replication of Rts1 and its inhibitory effect on the host cell are due to the presence of a gene or gene cluster on the Rts1 genome and that the gene(s) is clearly discriminated from the one responsible for the temperature sensitivity of transfer."}
{"id": "PMID:1090605", "title": "Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens.", "content": "Temperate phage Mu-1, which is able to integrate at random in its host chromosome, is also able to mediate integration of other circular deoxyribonucleic acid, as a lambda-gal mutant unable to integrate by itself. After mixed infection with lambda-gal and Mucplus, galplus transductants are recovered that have the lambda-gal integrated in any circular permutation, sandwiched between two complete Mu genomes in the same orientation, the whole Mu-lambda-gal-Mu structure being found at any location in the bacterial chromosome. Here we show that such a lambda-gal can integrate in an induced Mu lysogen. In this case the lambda-gal is again in any circular permutation, between two Mu in the same orientation, but it is always located at the site of the original Mu prophage, and the two surrounding Mu have always the same genotype as the original Mu prophage. Active Mu replication functions are not essential for that process to occur. This suggests that bacterial replication may generate two Mu copies that in some way can regenerate a Mu attachment site that recombines with the lambda-gal. A model is presented that accounts for these observations, may be helpful for understanding some complex features of Mu development, and may possibly offer a basis for explaining spontaneous duplications.", "contents": "Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens. Temperate phage Mu-1, which is able to integrate at random in its host chromosome, is also able to mediate integration of other circular deoxyribonucleic acid, as a lambda-gal mutant unable to integrate by itself. After mixed infection with lambda-gal and Mucplus, galplus transductants are recovered that have the lambda-gal integrated in any circular permutation, sandwiched between two complete Mu genomes in the same orientation, the whole Mu-lambda-gal-Mu structure being found at any location in the bacterial chromosome. Here we show that such a lambda-gal can integrate in an induced Mu lysogen. In this case the lambda-gal is again in any circular permutation, between two Mu in the same orientation, but it is always located at the site of the original Mu prophage, and the two surrounding Mu have always the same genotype as the original Mu prophage. Active Mu replication functions are not essential for that process to occur. This suggests that bacterial replication may generate two Mu copies that in some way can regenerate a Mu attachment site that recombines with the lambda-gal. A model is presented that accounts for these observations, may be helpful for understanding some complex features of Mu development, and may possibly offer a basis for explaining spontaneous duplications."}
{"id": "PMID:1090606", "title": "Accumulation of the capacity of initiation of deoxyribonucleic acid replication in Escherichia coli.", "content": "Several temperature-sensitive initiation mutants of Escherichia coli were examined for the ability to initiate more than one round of replication after being held at nonpermissive temperature for approximately 1.5 generation equivalents. The capacity for initiation was measured by residual synthesis experiments and rate experiments under conditions where protein synthesis and ribonucleic acid synthesis were inhibited. Results of the rate and density transfer experiments suggest that the cells may initiate more than one round of replication in the absence of protein or ribonucleic acid synthesis. This contrasts with the results of the residual synthesis experiments which suggest that, under these conditions, only one round of synthesis is achieved. These findings suggest that the total amount of residual synthesis achieved in the presence of an inhibitor may be both a function of the number of initiation events which occur and the effect of the inhibitor of protein or ribonucleic acid synthesis on chain elongation.", "contents": "Accumulation of the capacity of initiation of deoxyribonucleic acid replication in Escherichia coli. Several temperature-sensitive initiation mutants of Escherichia coli were examined for the ability to initiate more than one round of replication after being held at nonpermissive temperature for approximately 1.5 generation equivalents. The capacity for initiation was measured by residual synthesis experiments and rate experiments under conditions where protein synthesis and ribonucleic acid synthesis were inhibited. Results of the rate and density transfer experiments suggest that the cells may initiate more than one round of replication in the absence of protein or ribonucleic acid synthesis. This contrasts with the results of the residual synthesis experiments which suggest that, under these conditions, only one round of synthesis is achieved. These findings suggest that the total amount of residual synthesis achieved in the presence of an inhibitor may be both a function of the number of initiation events which occur and the effect of the inhibitor of protein or ribonucleic acid synthesis on chain elongation."}
{"id": "PMID:1090607", "title": "Temperature-sensitive recA mutant of Escherichia coli K-12: deoxyribonucleic acid metabolism after ultraviolet irradiation.", "content": "A mutant of Escherichia coli K-12 temperature sensitive for genetic recombination was investigated and found to carry a mutation that could be cotransduced with cysC and hence could be in the recA gene. To determine whether recA+ can complement this mutation, matings were carried out at 35 and 40 C between Hfr donors that transfer recA+ or recA1 early and recipients carrying wild-type or mutant alleles. It was found that recA+ but not recA1 complements this mutation in zygotic temporary partial diploids. The mutant allele was accordingly designated recA44. A transductant carrying recA44 behaved normally at low temperatures but more like recA- strains at high temperatures with respect to recombinant colony formation in Hfr matings, cell survival, and deoxyribonucleic acid (DNA) synthesis after ultraviolet irradiation, cellular DNA breakdown, and prophage induction when lysogenic for lambda. Alkaline sucrose sedimentation studies of DNA from recA44 cells showed that short DNA molecules synthesized immediately after ultraviolet irradiation increased in molecular weight during subsequent incubation at 32 C but not at 45 C. Hence, recA+ is required for this molecular weight increase. Cells exposed to ultraviolet light synthesized DNA that remained of low molecular weight during a 40-min incubation at 32 C. This material increased in molecular weight in recArut not in recA44 cells during subsequent incubation at 45 C. Thus, the availability of recA+ during the first 40 min at 32 C after irradiation did not obviate the need for recA+ in the subsequent phases of this post-replication repair process.", "contents": "Temperature-sensitive recA mutant of Escherichia coli K-12: deoxyribonucleic acid metabolism after ultraviolet irradiation. A mutant of Escherichia coli K-12 temperature sensitive for genetic recombination was investigated and found to carry a mutation that could be cotransduced with cysC and hence could be in the recA gene. To determine whether recA+ can complement this mutation, matings were carried out at 35 and 40 C between Hfr donors that transfer recA+ or recA1 early and recipients carrying wild-type or mutant alleles. It was found that recA+ but not recA1 complements this mutation in zygotic temporary partial diploids. The mutant allele was accordingly designated recA44. A transductant carrying recA44 behaved normally at low temperatures but more like recA- strains at high temperatures with respect to recombinant colony formation in Hfr matings, cell survival, and deoxyribonucleic acid (DNA) synthesis after ultraviolet irradiation, cellular DNA breakdown, and prophage induction when lysogenic for lambda. Alkaline sucrose sedimentation studies of DNA from recA44 cells showed that short DNA molecules synthesized immediately after ultraviolet irradiation increased in molecular weight during subsequent incubation at 32 C but not at 45 C. Hence, recA+ is required for this molecular weight increase. Cells exposed to ultraviolet light synthesized DNA that remained of low molecular weight during a 40-min incubation at 32 C. This material increased in molecular weight in recArut not in recA44 cells during subsequent incubation at 45 C. Thus, the availability of recA+ during the first 40 min at 32 C after irradiation did not obviate the need for recA+ in the subsequent phases of this post-replication repair process."}
{"id": "PMID:1090608", "title": "Dose dependence of the excision of ultraviolet-induced pyrimidine dimers from nuclear deoxyribonucleic acids of haploid and diploid Saccharomyces cerevisiae.", "content": "The yield of ultraviolet-induced dimers is similar for a fixed dose in both haploid and diploid Saccharomyces cerevisiae. The excision of these photo-products from the nuclear deoxyribonucleic acids of cells of both ploidies after ultraviolet incident doses of 2 times 10-3 to 4 times 10-3 ergs/mm2 decreased with the corresponding increasing dose. Postirradiation incubation in saline followed by a further incubation in nutrient medium increases the excision as compared to that seen in either nutrient medium or saline alone. Previous data regarding both pyrimidine dimer removal and the survival of haploid and diploid cells after ultraviolet irradiation and either immediate or delayed plating are discussed.", "contents": "Dose dependence of the excision of ultraviolet-induced pyrimidine dimers from nuclear deoxyribonucleic acids of haploid and diploid Saccharomyces cerevisiae. The yield of ultraviolet-induced dimers is similar for a fixed dose in both haploid and diploid Saccharomyces cerevisiae. The excision of these photo-products from the nuclear deoxyribonucleic acids of cells of both ploidies after ultraviolet incident doses of 2 times 10-3 to 4 times 10-3 ergs/mm2 decreased with the corresponding increasing dose. Postirradiation incubation in saline followed by a further incubation in nutrient medium increases the excision as compared to that seen in either nutrient medium or saline alone. Previous data regarding both pyrimidine dimer removal and the survival of haploid and diploid cells after ultraviolet irradiation and either immediate or delayed plating are discussed."}
{"id": "PMID:1090609", "title": "Strain of Escherichia coli with a temperature-sensitive mutation affecting ribosomal ribonucleic acid accumulation.", "content": "A mutant of Escherichia coli has been isolated that has a temperature-sensitive mutation that results in specific loss of ribosomal ribonucleic acid (RNA) synthesis and some reduction in messenger RNA synthesis. When the strain was grown in glucose medium at a restrictive temperature, RNA accumulation ceased, but both messenger RNA and protein synthesis continued for an extended time. Because carbon metabolism was slowed drastically when strain AA-157 was placed at the restrictive temperature, this phenotype can be compared with carbon depletion conditions present during diauxic lag. However, the phenotype of mutant AA-157 differs from shift-down conditions in that guanosine-3',5'-tetraphosphate levels are unaffected; therefore, a different site is affected. This mutant strain (AA-157) thus shows many characteristics similar to an aldolase mutant previously reported (B\u00f6ck and Neidhardt, 1966). However, the mutation occurred in a different position on the E. coli genetic map, and furthermore, aldolase was not temperature sensitive in strain AA-157. In this paper we present a study of macromolecular biosynthesis in this mutant.", "contents": "Strain of Escherichia coli with a temperature-sensitive mutation affecting ribosomal ribonucleic acid accumulation. A mutant of Escherichia coli has been isolated that has a temperature-sensitive mutation that results in specific loss of ribosomal ribonucleic acid (RNA) synthesis and some reduction in messenger RNA synthesis. When the strain was grown in glucose medium at a restrictive temperature, RNA accumulation ceased, but both messenger RNA and protein synthesis continued for an extended time. Because carbon metabolism was slowed drastically when strain AA-157 was placed at the restrictive temperature, this phenotype can be compared with carbon depletion conditions present during diauxic lag. However, the phenotype of mutant AA-157 differs from shift-down conditions in that guanosine-3',5'-tetraphosphate levels are unaffected; therefore, a different site is affected. This mutant strain (AA-157) thus shows many characteristics similar to an aldolase mutant previously reported (B\u00f6ck and Neidhardt, 1966). However, the mutation occurred in a different position on the E. coli genetic map, and furthermore, aldolase was not temperature sensitive in strain AA-157. In this paper we present a study of macromolecular biosynthesis in this mutant."}
{"id": "PMID:1090610", "title": "Adenylate energy charge in Saccharomyces cerevisiae during starvation.", "content": "Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown anaerobically on glucose and cells in the early stages of aerobic growth on glucose exhibit a rapid decrease of energy charge if transferred to medium lacking on energy source. These results suggest that functional mitochondria or enzymes of balance of adenine nucleotides during starvation. Yeast cells remain viable at energy charge values below 0.1, in marked contrast to results previously obtained with Escherichia coli. In other respects, the engery charge responses of yeast to starvation and refeeding are generally similar to those previously reported for E. coli.", "contents": "Adenylate energy charge in Saccharomyces cerevisiae during starvation. Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown anaerobically on glucose and cells in the early stages of aerobic growth on glucose exhibit a rapid decrease of energy charge if transferred to medium lacking on energy source. These results suggest that functional mitochondria or enzymes of balance of adenine nucleotides during starvation. Yeast cells remain viable at energy charge values below 0.1, in marked contrast to results previously obtained with Escherichia coli. In other respects, the engery charge responses of yeast to starvation and refeeding are generally similar to those previously reported for E. coli."}
{"id": "PMID:1090611", "title": "Bacteriophage resistance in Escherichia coli K-12: general pattern of resistance.", "content": "Resistant mutants were isolated to 42 virulent bacteriophages in one strain of Escherichia coli K-12 and tested for resistance or sensitivity to a set of 56 bacteriophages. Most of the mutants fell into 11 groups with respect to their resistance patterns. It was possible to classify the bacteriophages broadly, according to the variety of mutants that were resistant to them.", "contents": "Bacteriophage resistance in Escherichia coli K-12: general pattern of resistance. Resistant mutants were isolated to 42 virulent bacteriophages in one strain of Escherichia coli K-12 and tested for resistance or sensitivity to a set of 56 bacteriophages. Most of the mutants fell into 11 groups with respect to their resistance patterns. It was possible to classify the bacteriophages broadly, according to the variety of mutants that were resistant to them."}
{"id": "PMID:1090612", "title": "Regulation of ribosomal protein synthesis in Escherichia coli B/r.", "content": "The differential synthesis rate of ribosomal protein (r-protein), alpha-r (synthesis rate of r-protein divided by synthesis rate of total protein), was measured during the cell division cycle. It was observed that alpha-r remained essentially constant and was not measurably affected by duplication of the r-protein gene cluster (i.e., str-spc region) during the process of chromosome replication. It was further observed that the rate of total protein synthesis and r-protein synthesis increased continuously and uniformly during the entire cell cycle. This gene dosage independence of the synthesis rate of r-protein was similar to that observed earlier for the synthesis of ribosomal ribonucleic acid (rRNA). These observations indicate that the synthesis rates of the protein and RNA components of the ribosome are coordinately balanced during the entire cell division cycle and are not significantly perturbed by duplication of the r-protein or rRNA genes. Furthermore, this balanced synthesis insures that neither free rRNA nor free r-protein accumulate in appreciable amounts during balanced growth.", "contents": "Regulation of ribosomal protein synthesis in Escherichia coli B/r. The differential synthesis rate of ribosomal protein (r-protein), alpha-r (synthesis rate of r-protein divided by synthesis rate of total protein), was measured during the cell division cycle. It was observed that alpha-r remained essentially constant and was not measurably affected by duplication of the r-protein gene cluster (i.e., str-spc region) during the process of chromosome replication. It was further observed that the rate of total protein synthesis and r-protein synthesis increased continuously and uniformly during the entire cell cycle. This gene dosage independence of the synthesis rate of r-protein was similar to that observed earlier for the synthesis of ribosomal ribonucleic acid (rRNA). These observations indicate that the synthesis rates of the protein and RNA components of the ribosome are coordinately balanced during the entire cell division cycle and are not significantly perturbed by duplication of the r-protein or rRNA genes. Furthermore, this balanced synthesis insures that neither free rRNA nor free r-protein accumulate in appreciable amounts during balanced growth."}
{"id": "PMID:1090613", "title": "The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.", "content": "The DNA unwinding protein of Escherichia coli (Sigal, N., Delius, H., Kornberg, T., Gefter, M., and Alberts, B. (1972) Proc. Nat. Acad. Sci. U.S.A. 69, 3537-3541) has been purified to homogeneity by a simple procedure which utilizes its stability to heating. The protein is an asymmetric tetramer of 18,500 dalton subunits which binds preferentially to single-stranded DNA at a ratio of one protein molecule per 32 nucleotides. Binding to DNA is complete in less than 10 s at 0 degrees while release of the protein from single-stranded DNA is relatively slow even at 37 degrees. A simple functional assay for unwinding protein depends on its essential role in the conversion of phage G4 single-stranded DNA to the replicative form. Unwinding protein stimulates initiation of replication of all single-stranded phage DNAs. Approximately 300 copies of unwinding protein are present per cell, as estimated by antibody titration, an amount sufficient to cover substantial lengths of DNA in several replicating forks.", "contents": "The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication. The DNA unwinding protein of Escherichia coli (Sigal, N., Delius, H., Kornberg, T., Gefter, M., and Alberts, B. (1972) Proc. Nat. Acad. Sci. U.S.A. 69, 3537-3541) has been purified to homogeneity by a simple procedure which utilizes its stability to heating. The protein is an asymmetric tetramer of 18,500 dalton subunits which binds preferentially to single-stranded DNA at a ratio of one protein molecule per 32 nucleotides. Binding to DNA is complete in less than 10 s at 0 degrees while release of the protein from single-stranded DNA is relatively slow even at 37 degrees. A simple functional assay for unwinding protein depends on its essential role in the conversion of phage G4 single-stranded DNA to the replicative form. Unwinding protein stimulates initiation of replication of all single-stranded phage DNAs. Approximately 300 copies of unwinding protein are present per cell, as estimated by antibody titration, an amount sufficient to cover substantial lengths of DNA in several replicating forks."}
{"id": "PMID:1090614", "title": "Reactive half-cystine peptides of the secretory component of human exocrine immunoglobulin A.", "content": "On the basis of previous work the two forms of human secretory component, namely that which is covalently bound as a part of the exocrine immunoglobulin A molecule and the free form, are probably different states of the same protein. From autoradiographs of trypic peptide maps of bound and free secretory components which were radioactively alkylated after partial reduction, it was concluded that the same half-cystines in each are sensitive to reduction. in the present work the easily reduced half-cystines of the bound and free secretory components have been studies in more detail. In each form there are two such half-cystines. In the case of bound secretory component they provide the linkage to the remainder of the exocrine immunoglobulin A molecule. Peptides from the sensitive half-cystines were isolated from tryptic-peptic digests of free secretory component and sequenced. By diagonal electrophoresis these two peptides were shown to be joined in an intrachain disulfide bridge. Therefore, it is proposed that the exocrine immunoglobulin A molecule becomes fully assembled when a single, reactive intrachain disulfide bridge in free secretory component rearranges to yeild two interchain bridges with dimeric serum-type immunoglobulin A. This process is thought to occur within the epithelial lining cells of mucous membranes.", "contents": "Reactive half-cystine peptides of the secretory component of human exocrine immunoglobulin A. On the basis of previous work the two forms of human secretory component, namely that which is covalently bound as a part of the exocrine immunoglobulin A molecule and the free form, are probably different states of the same protein. From autoradiographs of trypic peptide maps of bound and free secretory components which were radioactively alkylated after partial reduction, it was concluded that the same half-cystines in each are sensitive to reduction. in the present work the easily reduced half-cystines of the bound and free secretory components have been studies in more detail. In each form there are two such half-cystines. In the case of bound secretory component they provide the linkage to the remainder of the exocrine immunoglobulin A molecule. Peptides from the sensitive half-cystines were isolated from tryptic-peptic digests of free secretory component and sequenced. By diagonal electrophoresis these two peptides were shown to be joined in an intrachain disulfide bridge. Therefore, it is proposed that the exocrine immunoglobulin A molecule becomes fully assembled when a single, reactive intrachain disulfide bridge in free secretory component rearranges to yeild two interchain bridges with dimeric serum-type immunoglobulin A. This process is thought to occur within the epithelial lining cells of mucous membranes."}
{"id": "PMID:1090615", "title": "Small ribonucleic acid molecules produced during ribosome biosynthesis in Saccharomyces cerevisiae.", "content": "A 5.8 S ribosomal RNA (rRNA) is found noncovalently bound to the 25 S RNA in the 60 S subunit of yeast ribosomes. Both molecules appear to be derived from the 27 S RNA, a ribosomal precursor species (Uden, S. A., and Warner, J. R. (1972) J. Mol. biol. 65, 227-242). We have detected two other RNA species by pulse labeling which may also be produced during 27 S RNA processing. The first is approximately 5.9 S and appears by kinetics of synthesis to be a direct precursor of 5.8 S rRNA. The second species is about 7 S, is absent from uniformly labeled RNA preparations, and is produced coincidentally with the 5.8 S rRNA. Both the 5.9 S RNA and the 7 S RNA are not produced in mutants defective in ribosome biosynthesis (Hartwell, L. H., McLaughlin, C. S., and Warner, J. R. (1970) Mol. Gen. Genet. 109, 42-56) which are unable to make the 25 S-5.8 S rRNA complex from 27 S RNA. Thus, the maturation of 27 S RNA appears to involve the production of a 7 S RNA, which could be either a precursor or a nonconserved RNA region, and a 5.9 S RNA, most of which is trimmed to give the mature 5.8 S rRNA in the 25 S-5.8 S rRNA complex of 60 S subunits.", "contents": "Small ribonucleic acid molecules produced during ribosome biosynthesis in Saccharomyces cerevisiae. A 5.8 S ribosomal RNA (rRNA) is found noncovalently bound to the 25 S RNA in the 60 S subunit of yeast ribosomes. Both molecules appear to be derived from the 27 S RNA, a ribosomal precursor species (Uden, S. A., and Warner, J. R. (1972) J. Mol. biol. 65, 227-242). We have detected two other RNA species by pulse labeling which may also be produced during 27 S RNA processing. The first is approximately 5.9 S and appears by kinetics of synthesis to be a direct precursor of 5.8 S rRNA. The second species is about 7 S, is absent from uniformly labeled RNA preparations, and is produced coincidentally with the 5.8 S rRNA. Both the 5.9 S RNA and the 7 S RNA are not produced in mutants defective in ribosome biosynthesis (Hartwell, L. H., McLaughlin, C. S., and Warner, J. R. (1970) Mol. Gen. Genet. 109, 42-56) which are unable to make the 25 S-5.8 S rRNA complex from 27 S RNA. Thus, the maturation of 27 S RNA appears to involve the production of a 7 S RNA, which could be either a precursor or a nonconserved RNA region, and a 5.9 S RNA, most of which is trimmed to give the mature 5.8 S rRNA in the 25 S-5.8 S rRNA complex of 60 S subunits."}
{"id": "PMID:1090616", "title": "The activity of oligonucleotides containing guanosine 5'-triphosphate in protein synthesis. I. The interaction of protein synthesis elongation factor I with cytidylyl (5'-3')-guanosine 5'-triphosphate.", "content": "The interaction of protein synthesis elongation factor 1 (EF-1) from wheat embryos and elongation factor Tu from Escherichia coli with cytidylyl(5'-3')guanosine 5'-triphosphate(pppGpC) has been studied. The dinucleotide 5'-triphosphate interacts strongly with EF-1 as evidenced by its capacity to inhibit the binding of [3H]GTP to the factor. The analogs pGpC and GpC do not interfere with GTP binding to EF-1 but guanosine 5'-triphosphate cyclic 2',3'-monophosphate and ppGpC are also potent inhibitors. The binding of the dinucleotide 5'-triphosphate to EF-1 was also demonstrated directly by the nitrocellulose retention method and by Sephadex G-50 fractionation using a radioactive analog iodinated with 125I in the 5 position of the cytosine of pppGpC. The dinucleotide triphosphate can replace GTP in the formation of a ternary complex EF-1-aminoacyl-tRNA-GTP and in its requirement for the binding of aminoacyl-tRNA to ribosomes catalyzed by EF-1. The absolute requirement for GTP in an in vitro polypeptide-synthesizing system can also be met by pppGpC and by guanosine 5'-triphosphate cyclic 5',3'-monophosphate. The bacterial factor EF-Tu differs drastically from eukaryotic EF-1 in its nucleotide specificity since EF-Tu only interacts slightly (if at all) with pppGpC. The low inhibition of [3H]GTP binding to EF-Tu by pppGpC could be due to a slight contamination in the latter compound.", "contents": "The activity of oligonucleotides containing guanosine 5'-triphosphate in protein synthesis. I. The interaction of protein synthesis elongation factor I with cytidylyl (5'-3')-guanosine 5'-triphosphate. The interaction of protein synthesis elongation factor 1 (EF-1) from wheat embryos and elongation factor Tu from Escherichia coli with cytidylyl(5'-3')guanosine 5'-triphosphate(pppGpC) has been studied. The dinucleotide 5'-triphosphate interacts strongly with EF-1 as evidenced by its capacity to inhibit the binding of [3H]GTP to the factor. The analogs pGpC and GpC do not interfere with GTP binding to EF-1 but guanosine 5'-triphosphate cyclic 2',3'-monophosphate and ppGpC are also potent inhibitors. The binding of the dinucleotide 5'-triphosphate to EF-1 was also demonstrated directly by the nitrocellulose retention method and by Sephadex G-50 fractionation using a radioactive analog iodinated with 125I in the 5 position of the cytosine of pppGpC. The dinucleotide triphosphate can replace GTP in the formation of a ternary complex EF-1-aminoacyl-tRNA-GTP and in its requirement for the binding of aminoacyl-tRNA to ribosomes catalyzed by EF-1. The absolute requirement for GTP in an in vitro polypeptide-synthesizing system can also be met by pppGpC and by guanosine 5'-triphosphate cyclic 5',3'-monophosphate. The bacterial factor EF-Tu differs drastically from eukaryotic EF-1 in its nucleotide specificity since EF-Tu only interacts slightly (if at all) with pppGpC. The low inhibition of [3H]GTP binding to EF-Tu by pppGpC could be due to a slight contamination in the latter compound."}
{"id": "PMID:1090617", "title": "Methylation and processing of transfer ribonucleic acid in mammalian and bacterial cells.", "content": "The relationship between the methylation and processing of tRNA in both bacterial and mammalian cell systems was investigated by assessing the methylation of an existing population of precursor-tRNAs in the absence of tRNA synthesis. When the synthesis of tRNA in Escherichia coli B (rifampicin) and human KB cells (actinomycin D) was inhibited with the appropriate antibiotic, the incorporation of [3H[methyl groups into tRNA (via [methyl-3H]methionine labeling) rapidly declined with time and was essentially complete within 30 and 60 min, respectively. Although antibiotic treatment predictably reduced the incorporation of methyl groups into tRNA, it also resulted in significant changes in the distribution of the type of methylated products formed. Thus, for KB cells the marked increases in the per cent of radioactivity incorporated into 2'-0-methylribose derivatives, N2-methylguanine, and 3-methylcytosine of tRNA preparation pre-chased with actinomycin D for progressively longer periods of time prior to labeling with [methyl-3H]methionine led to the interpretation that these methylated constituents were formed predominantly during the late stages of tRNA maturation. Similarly, progessive and marked decreases in 1-, 7-, and N2, N2-methylguanine, and moderate decreases in 1-methyladenosine, 5-methylcytosine, and 5-methyluracil revealed that these methylated products were formed primarily during the early and intermediate stages of maturation, respectively. Similar analysis of E. coli B methylation products indicated that the bulk of methyl groups incorporated into the base moieties of tRNA (1- and 7-methylguanine, 2- and N6-methyladenine, and 5-methyluracil) occurred prior to the formation of 2'-0-methylribose derivatives. Additional evidence is presented which negates the possibility that an ancillary action of these antibiotics was the inhibition of specific tRNA-methyl-transferase enzymes.", "contents": "Methylation and processing of transfer ribonucleic acid in mammalian and bacterial cells. The relationship between the methylation and processing of tRNA in both bacterial and mammalian cell systems was investigated by assessing the methylation of an existing population of precursor-tRNAs in the absence of tRNA synthesis. When the synthesis of tRNA in Escherichia coli B (rifampicin) and human KB cells (actinomycin D) was inhibited with the appropriate antibiotic, the incorporation of [3H[methyl groups into tRNA (via [methyl-3H]methionine labeling) rapidly declined with time and was essentially complete within 30 and 60 min, respectively. Although antibiotic treatment predictably reduced the incorporation of methyl groups into tRNA, it also resulted in significant changes in the distribution of the type of methylated products formed. Thus, for KB cells the marked increases in the per cent of radioactivity incorporated into 2'-0-methylribose derivatives, N2-methylguanine, and 3-methylcytosine of tRNA preparation pre-chased with actinomycin D for progressively longer periods of time prior to labeling with [methyl-3H]methionine led to the interpretation that these methylated constituents were formed predominantly during the late stages of tRNA maturation. Similarly, progessive and marked decreases in 1-, 7-, and N2, N2-methylguanine, and moderate decreases in 1-methyladenosine, 5-methylcytosine, and 5-methyluracil revealed that these methylated products were formed primarily during the early and intermediate stages of maturation, respectively. Similar analysis of E. coli B methylation products indicated that the bulk of methyl groups incorporated into the base moieties of tRNA (1- and 7-methylguanine, 2- and N6-methyladenine, and 5-methyluracil) occurred prior to the formation of 2'-0-methylribose derivatives. Additional evidence is presented which negates the possibility that an ancillary action of these antibiotics was the inhibition of specific tRNA-methyl-transferase enzymes."}
{"id": "PMID:1090618", "title": "Evidence for the allosteric regulation of glycogen synthesis in the intact Escherichia coli cell. Agreement of the values of the parameters of the Hill equation fitted to data for glycogen synthesis in vivo with the abailable values obtained in vitro with adenosine diphosphoglucose synthetase.", "content": "In various nutrient-limited cultures of either Escherichia coli W4597(K) or G34 a 10-fold range of rates of glycogen synthesis is observed while the energy charge values (0.86 plus or minus 0.01) and glucose 6-phosphate levels are essentially the same in each condition. The steady state level of fructose 1,6-diphosphate in these cultures varies from experiment to experiment as a function of the observed rate of glycogen synthesis. These data were fitted to the Hill equation by a nonlinear regression analysis and the statistically most probable values obtained for the Hill coefficient (n), A0.5, and V were, respectively, 2.08, 0.82mM, and 1030 mumol/g of protein per hour. The values of the first two parameters agree well with values available at energy charge 0.85 for the in vitro synthesis of ADPG by the ADPG synthetase of E. coli. When the difference in the glucose 1-phosphate concentration used in the studies in vitro from the apparent glucose 1-phosphate concentration in vivo (estimated from the glucose 6-phosphate levels) is considered, the in vitro value of V (1140 mumol of ADPG synthesized per g of protein per hour) is quite similar to the value of V (1030 mumol of glucose incorporated into glycogen per g of protein per hour) for glycogen synthesis in vivo. The close agreement of the values of the parameters of the Hill equation for glycogen synthesis in vivo to the values obtained for ADPG synthesis in vitro provides the most quantitative evidence yet obtained that allosteric regulation of bacterial glycogen synthesis functions in vivo.", "contents": "Evidence for the allosteric regulation of glycogen synthesis in the intact Escherichia coli cell. Agreement of the values of the parameters of the Hill equation fitted to data for glycogen synthesis in vivo with the abailable values obtained in vitro with adenosine diphosphoglucose synthetase. In various nutrient-limited cultures of either Escherichia coli W4597(K) or G34 a 10-fold range of rates of glycogen synthesis is observed while the energy charge values (0.86 plus or minus 0.01) and glucose 6-phosphate levels are essentially the same in each condition. The steady state level of fructose 1,6-diphosphate in these cultures varies from experiment to experiment as a function of the observed rate of glycogen synthesis. These data were fitted to the Hill equation by a nonlinear regression analysis and the statistically most probable values obtained for the Hill coefficient (n), A0.5, and V were, respectively, 2.08, 0.82mM, and 1030 mumol/g of protein per hour. The values of the first two parameters agree well with values available at energy charge 0.85 for the in vitro synthesis of ADPG by the ADPG synthetase of E. coli. When the difference in the glucose 1-phosphate concentration used in the studies in vitro from the apparent glucose 1-phosphate concentration in vivo (estimated from the glucose 6-phosphate levels) is considered, the in vitro value of V (1140 mumol of ADPG synthesized per g of protein per hour) is quite similar to the value of V (1030 mumol of glucose incorporated into glycogen per g of protein per hour) for glycogen synthesis in vivo. The close agreement of the values of the parameters of the Hill equation for glycogen synthesis in vivo to the values obtained for ADPG synthesis in vitro provides the most quantitative evidence yet obtained that allosteric regulation of bacterial glycogen synthesis functions in vivo."}
{"id": "PMID:1090619", "title": "Cleavage of Nonglucosylated Bacteriophage T4 deoxyribonucleic acid by Restriction Endonuclease Eco RI.", "content": "DNAs lacking the glucosyl modification (Glc-) and additionally lacking the 6-methylaminopurine (N6-methyladenine) modification (Glc-, MeAde-) were prepared from appropriate T4 mutants. These DNAs were cleaved by the purified restriction endonuclease Eco TI from Escherichia coli. Normally modified DNA (Glc+, MeAde+) was not attached. The Eco RII and the hemophilus enzymes Hin dII and Hin dIII do not attack Glc-, MeAde- T DNA, possibly due to the presence of 6-hydroxymethylcytosine. Eco RI produces approximately 40 specific fragments from Glc- DNA ranging in molecular weights from 0.3 to 10.5 X 10-6.", "contents": "Cleavage of Nonglucosylated Bacteriophage T4 deoxyribonucleic acid by Restriction Endonuclease Eco RI. DNAs lacking the glucosyl modification (Glc-) and additionally lacking the 6-methylaminopurine (N6-methyladenine) modification (Glc-, MeAde-) were prepared from appropriate T4 mutants. These DNAs were cleaved by the purified restriction endonuclease Eco TI from Escherichia coli. Normally modified DNA (Glc+, MeAde+) was not attached. The Eco RII and the hemophilus enzymes Hin dII and Hin dIII do not attack Glc-, MeAde- T DNA, possibly due to the presence of 6-hydroxymethylcytosine. Eco RI produces approximately 40 specific fragments from Glc- DNA ranging in molecular weights from 0.3 to 10.5 X 10-6."}
{"id": "PMID:1090620", "title": "Escherichia coli ribosomal ribonucleic acids are not cut from an intact precursor molecule.", "content": "Ribonuclease III-deficient strains of Escherichia coli accumulate a \"30 S\" RNA species. Kinetic analysis of label incorporated into rRNA species shows, however, that 30 S RNA is not the major precursor to the 16 S and 23 S RNA species is in 7- to 10-fold excess over that into 30 S RNA. The 30 S RNA species turns over with a half-life of about 2.5 min, which could account for no more than one-tenth of the incorporation into 16 S plus 23 S RNA. Thus, under these conditions RNase III-strains of E. coli do not cut rRNAs from an intact tandem precursor molecule but rather from the elongating nascent transcript, possibly by an alternate pathway not involving RNase III.", "contents": "Escherichia coli ribosomal ribonucleic acids are not cut from an intact precursor molecule. Ribonuclease III-deficient strains of Escherichia coli accumulate a \"30 S\" RNA species. Kinetic analysis of label incorporated into rRNA species shows, however, that 30 S RNA is not the major precursor to the 16 S and 23 S RNA species is in 7- to 10-fold excess over that into 30 S RNA. The 30 S RNA species turns over with a half-life of about 2.5 min, which could account for no more than one-tenth of the incorporation into 16 S plus 23 S RNA. Thus, under these conditions RNase III-strains of E. coli do not cut rRNAs from an intact tandem precursor molecule but rather from the elongating nascent transcript, possibly by an alternate pathway not involving RNase III."}
{"id": "PMID:1090627", "title": "Observations on the induction of bone in soft tissues.", "content": "Using bone decalcified with 0-6 N hydrochloric acid as an inducing agent, the inductive capacity of different soft tissue sites was investigated. Muscle and fascia regularly permitted the induction of bone, while spleen, liver and kidney suppressed bone induction. Bone formation could be induced in these organs if living autologous fascia was implanted together with the inducing agent; while bone formation was inhibited when living autologous spleen tissue was implanted with the inducing agent to normally favourable sites. The administration of systemic heparin and the diphosphonate ethane-1-hydroxyl, 1-diphosphonic acid (EHDP) suppressed bone induction. It is suggested that for bone induction to occur in soft tissues, three conditions must be present: 1) an inducing agent; 2) an osteogenic precursor cell; and 3) an environment which is permissive to osteogenesis. The presence of osteogenic inhibitors in spleen, liver and kidney is postulated.", "contents": "Observations on the induction of bone in soft tissues. Using bone decalcified with 0-6 N hydrochloric acid as an inducing agent, the inductive capacity of different soft tissue sites was investigated. Muscle and fascia regularly permitted the induction of bone, while spleen, liver and kidney suppressed bone induction. Bone formation could be induced in these organs if living autologous fascia was implanted together with the inducing agent; while bone formation was inhibited when living autologous spleen tissue was implanted with the inducing agent to normally favourable sites. The administration of systemic heparin and the diphosphonate ethane-1-hydroxyl, 1-diphosphonic acid (EHDP) suppressed bone induction. It is suggested that for bone induction to occur in soft tissues, three conditions must be present: 1) an inducing agent; 2) an osteogenic precursor cell; and 3) an environment which is permissive to osteogenesis. The presence of osteogenic inhibitors in spleen, liver and kidney is postulated."}
{"id": "PMID:1090628", "title": "Posterior psoas transfer and hip instability in lumbar myelomeningocele.", "content": "Seventy-two posterior psoas transfer operations performed in forty-four children with lumbar myelomeningocele were reviewed one to eight years after operation in an attempt to assess its value. Muscle charting, an objective recording of the child's walking ability, and radiographic examination of the hips were done. Hip stability was improved: 49 per cent were stable at the time of psoas transfer and 94 per cent at review. Functional results depend mainly on the level of neurological activity present: 57 per cent of the children had an acceptable functional result. Usually, posterior psoas transfer should be done as soon after the age of nine months as the child's condition will allow. Over the age of two years it should be restricted to children with activity in the third and fourth segments of the lumbar cord.", "contents": "Posterior psoas transfer and hip instability in lumbar myelomeningocele. Seventy-two posterior psoas transfer operations performed in forty-four children with lumbar myelomeningocele were reviewed one to eight years after operation in an attempt to assess its value. Muscle charting, an objective recording of the child's walking ability, and radiographic examination of the hips were done. Hip stability was improved: 49 per cent were stable at the time of psoas transfer and 94 per cent at review. Functional results depend mainly on the level of neurological activity present: 57 per cent of the children had an acceptable functional result. Usually, posterior psoas transfer should be done as soon after the age of nine months as the child's condition will allow. Over the age of two years it should be restricted to children with activity in the third and fourth segments of the lumbar cord."}
{"id": "PMID:1090630", "title": "The basal apparatus. Mass isolation from the molluscan ciliated gill epithelium and a preliminary characterization of striated rootlets.", "content": "The basal apparatus, consisting of an array of interconnected basal bodies bearing bifurcating striated rootlets encompassing a nucleus, has been isolated from hypertonically deciliated columnar gill epithelial cells of the bay scallop Aequipecten irradians through gentle lysis with Triton X-100. The rootlets, 8-10 mum in length, were not easily preserved with conventional electron microscope fixatives, suggesting that the extent of their contribution to cellular architecture has been somewhat underestimated, even though Englemann described many of the structural details of the basal apparatus in 1880. The striated rootlets were soluble at high but not at low pH, in 2 M solutions of sodium azide and potassium thiocyanate but not sodium or potassium chloride, in 1% deoxycholate but not digitonin, and in the denaturing solvents 6 M guanidine-HC1, 8 M urea, and 1% sodium dodecylsulfate at 100 degrees C. The protein found consistently when rootlets were solubilized migrated on SDS-polyacrylamide gels as a closely spaced doublet with apparent molecular weights of 230,000 and 250,000 daltons. This unique protein, distinct from tropocollagen or various muscle components, has been named ankyrin because of the rootlet's anchor-like function in the cell.", "contents": "The basal apparatus. Mass isolation from the molluscan ciliated gill epithelium and a preliminary characterization of striated rootlets. The basal apparatus, consisting of an array of interconnected basal bodies bearing bifurcating striated rootlets encompassing a nucleus, has been isolated from hypertonically deciliated columnar gill epithelial cells of the bay scallop Aequipecten irradians through gentle lysis with Triton X-100. The rootlets, 8-10 mum in length, were not easily preserved with conventional electron microscope fixatives, suggesting that the extent of their contribution to cellular architecture has been somewhat underestimated, even though Englemann described many of the structural details of the basal apparatus in 1880. The striated rootlets were soluble at high but not at low pH, in 2 M solutions of sodium azide and potassium thiocyanate but not sodium or potassium chloride, in 1% deoxycholate but not digitonin, and in the denaturing solvents 6 M guanidine-HC1, 8 M urea, and 1% sodium dodecylsulfate at 100 degrees C. The protein found consistently when rootlets were solubilized migrated on SDS-polyacrylamide gels as a closely spaced doublet with apparent molecular weights of 230,000 and 250,000 daltons. This unique protein, distinct from tropocollagen or various muscle components, has been named ankyrin because of the rootlet's anchor-like function in the cell."}
{"id": "PMID:1090629", "title": "Messenger RNA metabolism of animal cells. Possible involvement of untranslated sequences and mRNA-associated proteins.", "content": "The past several years have seen a virtual revolution in the study of eukaryotic mRNA. Among the notable recent achievements are the positive identification of mRNA precursors in HnRNA, the enumeration of the DNA sequences from which mRNA is transcribed, and the finding that mRNA in cultured cells is much more stable than was previously believed. One of most far-reaching discoveries has been the finding that mRNA in eukaryotes contains poly A. This discovery, aside from providing a powerful tool for mRNA isolation, has generated a large body of research into the properties and metabolism of poly A itself. In addition, the finding of a poly A-associated protein has given a renewed stimulus to the study of proteins associated with mRNA. This review is devoted to a discussion of these and related achievements, and some of their implications", "contents": "Messenger RNA metabolism of animal cells. Possible involvement of untranslated sequences and mRNA-associated proteins. The past several years have seen a virtual revolution in the study of eukaryotic mRNA. Among the notable recent achievements are the positive identification of mRNA precursors in HnRNA, the enumeration of the DNA sequences from which mRNA is transcribed, and the finding that mRNA in cultured cells is much more stable than was previously believed. One of most far-reaching discoveries has been the finding that mRNA in eukaryotes contains poly A. This discovery, aside from providing a powerful tool for mRNA isolation, has generated a large body of research into the properties and metabolism of poly A itself. In addition, the finding of a poly A-associated protein has given a renewed stimulus to the study of proteins associated with mRNA. This review is devoted to a discussion of these and related achievements, and some of their implications"}
{"id": "PMID:1090631", "title": "Mitosis in the cellular slime mold Polysphondylium violaceum.", "content": "Myxamebas of Polysphondylium violaceum were grown in liquid medium and processed for electron microscopy. Mitosis is characterized by a persistent nuclear envelope, ring-shaped extranuclear spindle pole bodies (SPBs), a central spindle spatially separated from the chromosomal microtubules, well-differentiated kinetochores, and dispersion of the nucleoli. SPBs originate from the division, during prophase, of an electron-opaque body associated with the interphase nucleus. The nuclear nevelope becomes fenestrated in their vicinity, allowing the build-up of the intranuclear, central spindle and chromosomal microtubules as the SPBs migrate to opposite poles. At metaphase the chromosomes are in amphitelic orientation, each sister chromatid being directly connected to the corresponding SPB by a single microtubule. During ana- and telophase the central spindle elongates, the daughter chromosomes approach the SPBs, and the nucleus constricts in the equatorial region. The cytoplasm cleaves by furrowing in late telophase, which is in other respects characterized by a re-establishment of the interphase condition. Spindle elongation and poleward movement of chromosomes are discussed in relation to hypotheses of the mechanism of mitosis.", "contents": "Mitosis in the cellular slime mold Polysphondylium violaceum. Myxamebas of Polysphondylium violaceum were grown in liquid medium and processed for electron microscopy. Mitosis is characterized by a persistent nuclear envelope, ring-shaped extranuclear spindle pole bodies (SPBs), a central spindle spatially separated from the chromosomal microtubules, well-differentiated kinetochores, and dispersion of the nucleoli. SPBs originate from the division, during prophase, of an electron-opaque body associated with the interphase nucleus. The nuclear nevelope becomes fenestrated in their vicinity, allowing the build-up of the intranuclear, central spindle and chromosomal microtubules as the SPBs migrate to opposite poles. At metaphase the chromosomes are in amphitelic orientation, each sister chromatid being directly connected to the corresponding SPB by a single microtubule. During ana- and telophase the central spindle elongates, the daughter chromosomes approach the SPBs, and the nucleus constricts in the equatorial region. The cytoplasm cleaves by furrowing in late telophase, which is in other respects characterized by a re-establishment of the interphase condition. Spindle elongation and poleward movement of chromosomes are discussed in relation to hypotheses of the mechanism of mitosis."}
{"id": "PMID:1090632", "title": "Ultrastructural immunoperoxidase demonstration of autologous albumin in the alveolar capillary membrane and in the alveolar lining material in normal rats.", "content": "The location of autologous serum albumin within the alveolar-capillary membrane was studied in the rat under physiological conditions using antialbumin antibodies labeled with peroxidase. Albumin was detected in the lung interstitium, and in numerous pinocytic vesicles within endothelial cells and type I alveolar epithelial cells. The immunoreaction was also positive at the level of plasmalemmal membranes of both cell types and in the alveolar lining material.", "contents": "Ultrastructural immunoperoxidase demonstration of autologous albumin in the alveolar capillary membrane and in the alveolar lining material in normal rats. The location of autologous serum albumin within the alveolar-capillary membrane was studied in the rat under physiological conditions using antialbumin antibodies labeled with peroxidase. Albumin was detected in the lung interstitium, and in numerous pinocytic vesicles within endothelial cells and type I alveolar epithelial cells. The immunoreaction was also positive at the level of plasmalemmal membranes of both cell types and in the alveolar lining material."}
{"id": "PMID:1090634", "title": "Thermal chromatography of lysine-specific transfer ribonucleic acid from Escherichia coli B.", "content": "Two Escherichia coli B tRNALys isoacceptors have been separated by thermal chromatography on hydroxyapatite demonstrating the potential of this approach in the fractionation of single-stranded polynucleotides. Elution temperatures were reproducible and depended primarily on the cation present and its concentration. Cesium phosphate was a more effective eluent than sodium phosphate. The integrity of the polynucleotide following thermal chromatography was examined by ultracentrifugation in a 60% dimethyl sulfoxide solvent, electrophoresis on polyacrylamide gels containing urea and oligonucleotide mapping. Adsorption to hydroxyapatite did not noticeably increase phosphodiester bond breakage compared with that in solution. Minimal, if any, rupture of covalent linkages was detected under the conditions of preparative separation of the isoacceptors. Several observations suggest that only one tRNALys was transcribed by the E. coli B cell.", "contents": "Thermal chromatography of lysine-specific transfer ribonucleic acid from Escherichia coli B. Two Escherichia coli B tRNALys isoacceptors have been separated by thermal chromatography on hydroxyapatite demonstrating the potential of this approach in the fractionation of single-stranded polynucleotides. Elution temperatures were reproducible and depended primarily on the cation present and its concentration. Cesium phosphate was a more effective eluent than sodium phosphate. The integrity of the polynucleotide following thermal chromatography was examined by ultracentrifugation in a 60% dimethyl sulfoxide solvent, electrophoresis on polyacrylamide gels containing urea and oligonucleotide mapping. Adsorption to hydroxyapatite did not noticeably increase phosphodiester bond breakage compared with that in solution. Minimal, if any, rupture of covalent linkages was detected under the conditions of preparative separation of the isoacceptors. Several observations suggest that only one tRNALys was transcribed by the E. coli B cell."}
{"id": "PMID:1090635", "title": "Assessment of pituitary gonadotropin reserve using luteinizing hormone-releasing hormone (LRH) in states of altered thyroid function.", "content": "Menstrual irregularities commonly accompany hyper- and hypofunction of the thyroid gland. Investigation of the pituitary-ovarian axis was undertaken in 14 hyperthyroid and 8 hypothyroid patients. The pituitary luteinizing hormone (LH) and follicle stimulating hormone (FSH) responses to 100 mug luteinizing hormone-releasing hormone (LRH) was assessed. These responses were found to be similar to matched control groups in hyperthyroid premenopausal women with and without menstrual disturbances and in hyperthyroid men. The postmenopausal hyperthyroid women had an exaggerated LH response, although the FSH response was comparable to that of the control group. These results indicate normal pituitary LH and FSH reserve in hyperthyroidism. In hypothyroidism a gradation of gonadotropin response to LRH was found. In 2 of the patients there was an inadequate LH response in the presence of a normal FSH response. This demonstrates that in some cases of primary hypothyroidism there is limited pituitary LH reserve.", "contents": "Assessment of pituitary gonadotropin reserve using luteinizing hormone-releasing hormone (LRH) in states of altered thyroid function. Menstrual irregularities commonly accompany hyper- and hypofunction of the thyroid gland. Investigation of the pituitary-ovarian axis was undertaken in 14 hyperthyroid and 8 hypothyroid patients. The pituitary luteinizing hormone (LH) and follicle stimulating hormone (FSH) responses to 100 mug luteinizing hormone-releasing hormone (LRH) was assessed. These responses were found to be similar to matched control groups in hyperthyroid premenopausal women with and without menstrual disturbances and in hyperthyroid men. The postmenopausal hyperthyroid women had an exaggerated LH response, although the FSH response was comparable to that of the control group. These results indicate normal pituitary LH and FSH reserve in hyperthyroidism. In hypothyroidism a gradation of gonadotropin response to LRH was found. In 2 of the patients there was an inadequate LH response in the presence of a normal FSH response. This demonstrates that in some cases of primary hypothyroidism there is limited pituitary LH reserve."}
{"id": "PMID:1090636", "title": "Release of colony-stimulating activity from thymus-derived lymphocytes.", "content": "Colony-stimulating activity (CSA) is essential for in vitro differentiation of bone marrow cells into colonies of granulocytes and mononuclear cells. While blood monocytes and macrophages are a major source of CSA, recent studies have indicated that CSA may be produced by lymphocytes responding to immunologic stimulation. Lymphocytes, purified from spleens and thymuses of mice by glass wool columns, were incubated in CMRL-1066 medium with fetal calf serum in vitro. Lymphocytes from the thumus and spleen released CSA when cultured in vitro, with peak levels of CSA observed after 7 days of incubation. Stimulation of cultures with phytohemagglutinin, concanavalin A, or pokeweed mitogen resulted in a 2-5-fold increase in CSA release, with peak levels of CSA released after 4 days of incubation. Thymus-dependent lymphocytes were responsible for the release of CSA from unstimulated and mitogen-stimulated cultures, since the incubation of these cultures with rabbit anti-mouse T cell sera abolished their ability to release CSA. Anti-mouse B cell sera had no effect on the ability of lymphocyte cultures to release CSA. These studies suggest that thymocytes and thymus-derived lymphocytes can release CSA in vitro and may be responsible for the increase in CSA observed in certain immunologic reactions.", "contents": "Release of colony-stimulating activity from thymus-derived lymphocytes. Colony-stimulating activity (CSA) is essential for in vitro differentiation of bone marrow cells into colonies of granulocytes and mononuclear cells. While blood monocytes and macrophages are a major source of CSA, recent studies have indicated that CSA may be produced by lymphocytes responding to immunologic stimulation. Lymphocytes, purified from spleens and thymuses of mice by glass wool columns, were incubated in CMRL-1066 medium with fetal calf serum in vitro. Lymphocytes from the thumus and spleen released CSA when cultured in vitro, with peak levels of CSA observed after 7 days of incubation. Stimulation of cultures with phytohemagglutinin, concanavalin A, or pokeweed mitogen resulted in a 2-5-fold increase in CSA release, with peak levels of CSA released after 4 days of incubation. Thymus-dependent lymphocytes were responsible for the release of CSA from unstimulated and mitogen-stimulated cultures, since the incubation of these cultures with rabbit anti-mouse T cell sera abolished their ability to release CSA. Anti-mouse B cell sera had no effect on the ability of lymphocyte cultures to release CSA. These studies suggest that thymocytes and thymus-derived lymphocytes can release CSA in vitro and may be responsible for the increase in CSA observed in certain immunologic reactions."}
{"id": "PMID:1090637", "title": "The mechanism of acute renal failure after uranyl nitrate.", "content": "Administration of 25 mg/kg uranyl nitrate (UN) to rats leads to a brief period of polyuria followed by progressive oliguria with death at 5 days. Factors that determine glomerular filtration rate (GFR) were examined in control Munich-Wistar rats (n equals 16) and 2 h after either 15 mg/kg (n equals 8) or 25 mg/kg (n equals 7) of UN (i.v.) utilizing direct measurements of hydrostatic and oncotic pressures and plasma flow. Total kidney GFR was reduced to 47% of control in the low dose group and to 21% in the high dose group. The simultaneous nephron filtration rate (sngfr) was 28.6 plus or minus 0.8 nl/min/g kidney wt in control, 29.1 plus or minus 1.0 in the low dose group, and 18.1 plus or minus 1.2 (P less than 0.001) in the higher dose group. This disparity in UN effect upon GFR and sngfr was due to tubular back-diffusion of solute through damaged epithelia beyond the early proximal tubule as demonstrated by microinjection of inulin and mannitol in the proximal tubule. Inulin \"leak\" persisted at 6 h after UN when tubular pressure had returned to normal. Comparison of sngfr measured in early vs. late proximal tubule revealed no difference after high dose UN, suggesting no significant leak of inulin from the early proximal tubule, and that the decreased sngfr was due to primary reductions in ultrafiltration. Nephron plasma flow was equal to control at both doses of UN. Also directly measured hydrostatic pressure gradient across the glomerular capillary was not changed. The effective filtration pressure achieved equilibrium in control of animals but became significantly positive at the efferent end of the capillary at both doses of UN and increased. Total glomerular permeability (LpA) was progressively reduced from control (0.089 plus or minus 0.005 nl/s/g kidney wt/mm Hg) at low dose UN (0.047 plus or minus 0.013) and high dose 0.024 plus or minus 0.003 nl/s/g kidney wt/mm Hg). Therefore UN decreases GFR by two mechanisms: (1) tubular damage leading to back-diffusion of solutes and (b) a primary reduction in sngfr due to reduced LpA.", "contents": "The mechanism of acute renal failure after uranyl nitrate. Administration of 25 mg/kg uranyl nitrate (UN) to rats leads to a brief period of polyuria followed by progressive oliguria with death at 5 days. Factors that determine glomerular filtration rate (GFR) were examined in control Munich-Wistar rats (n equals 16) and 2 h after either 15 mg/kg (n equals 8) or 25 mg/kg (n equals 7) of UN (i.v.) utilizing direct measurements of hydrostatic and oncotic pressures and plasma flow. Total kidney GFR was reduced to 47% of control in the low dose group and to 21% in the high dose group. The simultaneous nephron filtration rate (sngfr) was 28.6 plus or minus 0.8 nl/min/g kidney wt in control, 29.1 plus or minus 1.0 in the low dose group, and 18.1 plus or minus 1.2 (P less than 0.001) in the higher dose group. This disparity in UN effect upon GFR and sngfr was due to tubular back-diffusion of solute through damaged epithelia beyond the early proximal tubule as demonstrated by microinjection of inulin and mannitol in the proximal tubule. Inulin \"leak\" persisted at 6 h after UN when tubular pressure had returned to normal. Comparison of sngfr measured in early vs. late proximal tubule revealed no difference after high dose UN, suggesting no significant leak of inulin from the early proximal tubule, and that the decreased sngfr was due to primary reductions in ultrafiltration. Nephron plasma flow was equal to control at both doses of UN. Also directly measured hydrostatic pressure gradient across the glomerular capillary was not changed. The effective filtration pressure achieved equilibrium in control of animals but became significantly positive at the efferent end of the capillary at both doses of UN and increased. Total glomerular permeability (LpA) was progressively reduced from control (0.089 plus or minus 0.005 nl/s/g kidney wt/mm Hg) at low dose UN (0.047 plus or minus 0.013) and high dose 0.024 plus or minus 0.003 nl/s/g kidney wt/mm Hg). Therefore UN decreases GFR by two mechanisms: (1) tubular damage leading to back-diffusion of solutes and (b) a primary reduction in sngfr due to reduced LpA."}
{"id": "PMID:1090644", "title": "Provisional tables on the zinc content of foods.", "content": "A critical and exhaustive review of published and unpublished data on the zinc content of foods showed that, with few exceptions, the major dietary sources of zinc were foods of animal origin. Oysters, which contained more than 5 mg. zinc per 3-oz. cooked portion, were an outstanding source of zinc. Lean beef and beef liver were also among the foods highest in zinc and ground beef was a good source. Dark meat of poultry contained more zinc than light meat, and turkey was higher in zinc than chicken. Egg yolk, but not egg white, was relatively high in zinc, as were nonfat dry milk and Cheddar cheese. Foods of plant origin, like those of animal origin, varied widely in zinc content. Cocoa and the milling fractions of bran and germ of wheat were high in zinc. Seeds of plants, such as muture dry legumes and peanuts, were better sources of zinc than were leaves, stalks, fruits, or roots. Vegetables, fruits, oils, fats, tea, coffee, and carbonated beverages contained little zinc. Factors influencing the zinc content of foods were evaluated. Provisional tables, giving representative values for zinc in more than two hundred foods, both in 100-gm. portions and in common household units, based on this research, are presented.", "contents": "Provisional tables on the zinc content of foods. A critical and exhaustive review of published and unpublished data on the zinc content of foods showed that, with few exceptions, the major dietary sources of zinc were foods of animal origin. Oysters, which contained more than 5 mg. zinc per 3-oz. cooked portion, were an outstanding source of zinc. Lean beef and beef liver were also among the foods highest in zinc and ground beef was a good source. Dark meat of poultry contained more zinc than light meat, and turkey was higher in zinc than chicken. Egg yolk, but not egg white, was relatively high in zinc, as were nonfat dry milk and Cheddar cheese. Foods of plant origin, like those of animal origin, varied widely in zinc content. Cocoa and the milling fractions of bran and germ of wheat were high in zinc. Seeds of plants, such as muture dry legumes and peanuts, were better sources of zinc than were leaves, stalks, fruits, or roots. Vegetables, fruits, oils, fats, tea, coffee, and carbonated beverages contained little zinc. Factors influencing the zinc content of foods were evaluated. Provisional tables, giving representative values for zinc in more than two hundred foods, both in 100-gm. portions and in common household units, based on this research, are presented."}
{"id": "PMID:1090640", "title": "Temperature ofthe pulp chamber during impression taking of full crown preparations with modelling compound.", "content": "Temperature elevations were measured in the pulp chamber of full cast crown preparations during impression taking with modelling compound in copper bands. Uncontrolled flaming, controlled flaming, and water heating of the bands produced temperatures of 53, 44, and 41.5 C, respectively. The time involved in restoring the temperature in the pulp to 37 C was determined. The implications of delayed chilling are discussed.", "contents": "Temperature ofthe pulp chamber during impression taking of full crown preparations with modelling compound. Temperature elevations were measured in the pulp chamber of full cast crown preparations during impression taking with modelling compound in copper bands. Uncontrolled flaming, controlled flaming, and water heating of the bands produced temperatures of 53, 44, and 41.5 C, respectively. The time involved in restoring the temperature in the pulp to 37 C was determined. The implications of delayed chilling are discussed."}
{"id": "PMID:1090639", "title": "Effect of ultrasonic ZnPO-4 cement removal on band adhesion and cement solubility under orthodontic bands.", "content": "The use of ultrasonic energy to remove excess ZnPO-4 cement from orthodontic bands reduces the solubility of the cement but does not affect band adhesion unless excessive band contact is made. Excessive band contact weakens band-to-tooth adhesion. The solubility of the cement is increased by decreasing the pH and is increased when organic anions capable of binding metal ions are present.", "contents": "Effect of ultrasonic ZnPO-4 cement removal on band adhesion and cement solubility under orthodontic bands. The use of ultrasonic energy to remove excess ZnPO-4 cement from orthodontic bands reduces the solubility of the cement but does not affect band adhesion unless excessive band contact is made. Excessive band contact weakens band-to-tooth adhesion. The solubility of the cement is increased by decreasing the pH and is increased when organic anions capable of binding metal ions are present."}
{"id": "PMID:1090646", "title": "Fluorescent antibody localization of the vitamin D-dependent calcium-binding protein in the oviduct of the laying hen.", "content": "Fluorescein isothiocyanate-conjugated sheep antirabbit gamma-globulin was used in the indirect fluorescent antibody technique to localize the vitamin D-dependent calcium-binding protein (CaBP) in the shell gland of the laying hen. Intense specific fluorescence, indicative of the presence of CaBP, was found only in the tubular gland cells and not in the surface epithelium. CaBP was primarily associated with the luminal surface and other cell boundaries, and in lesser concentrations within the cells except for occasional intracellular regions of high concentration. The magnum, isthmus and vagina were essentially negative. Since the concentrations of intestinal CaBP is highly and directly correlated with the efficiency of calcium absorption, the present results suggest that the tubular gland cells might have a primary role in the transport of calcium associated with egg shell formation.", "contents": "Fluorescent antibody localization of the vitamin D-dependent calcium-binding protein in the oviduct of the laying hen. Fluorescein isothiocyanate-conjugated sheep antirabbit gamma-globulin was used in the indirect fluorescent antibody technique to localize the vitamin D-dependent calcium-binding protein (CaBP) in the shell gland of the laying hen. Intense specific fluorescence, indicative of the presence of CaBP, was found only in the tubular gland cells and not in the surface epithelium. CaBP was primarily associated with the luminal surface and other cell boundaries, and in lesser concentrations within the cells except for occasional intracellular regions of high concentration. The magnum, isthmus and vagina were essentially negative. Since the concentrations of intestinal CaBP is highly and directly correlated with the efficiency of calcium absorption, the present results suggest that the tubular gland cells might have a primary role in the transport of calcium associated with egg shell formation."}
{"id": "PMID:1090648", "title": "Niridazole. II. A potent long-acting suppressant of cellular hypersensitivity.", "content": "Niridazole, a human anthelminthic drug, is shown to be a potent long-acting suppressor of delayed hypersensitivity. Much lower doses than used in the therapy of helminth infections suppressed granuloma formation around Schistosoma mansoni eggs for periods up to 32 days and inhibited delayed footpad swelling in mice previously sensitized with eggs. Marked prolongation of skin allograft survival in mice accross the H-2 area was produced. The drug is administered orally, has been used clinically on a massive scale, and appears to be relatively free of the cytotoxic effects of most immunosuppressive agents.", "contents": "Niridazole. II. A potent long-acting suppressant of cellular hypersensitivity. Niridazole, a human anthelminthic drug, is shown to be a potent long-acting suppressor of delayed hypersensitivity. Much lower doses than used in the therapy of helminth infections suppressed granuloma formation around Schistosoma mansoni eggs for periods up to 32 days and inhibited delayed footpad swelling in mice previously sensitized with eggs. Marked prolongation of skin allograft survival in mice accross the H-2 area was produced. The drug is administered orally, has been used clinically on a massive scale, and appears to be relatively free of the cytotoxic effects of most immunosuppressive agents."}
{"id": "PMID:1090649", "title": "Increased risistance of immunoglobulin A dimers to proteolytic degradation after binding of secretory component.", "content": "The contribution of secretory component to the stability of secretory IgA against proteolysis has been studied by a new approach, i.e., by comparing the proteolytic degradation of the complexes formed in vitro between these proteins and secretory component. The results show that attachment of secretory components to the dimeric backbone of the secretory IgA molecule is accompanied by a significantly increased resistance of this backbone against digestion by both trypsin and pepsin. This protective effect may be a physiologic function of secretory component or may be due merely to unspecific blocking by secretory component of one or more sensitive peptide bonds in the IgA backbone.", "contents": "Increased risistance of immunoglobulin A dimers to proteolytic degradation after binding of secretory component. The contribution of secretory component to the stability of secretory IgA against proteolysis has been studied by a new approach, i.e., by comparing the proteolytic degradation of the complexes formed in vitro between these proteins and secretory component. The results show that attachment of secretory components to the dimeric backbone of the secretory IgA molecule is accompanied by a significantly increased resistance of this backbone against digestion by both trypsin and pepsin. This protective effect may be a physiologic function of secretory component or may be due merely to unspecific blocking by secretory component of one or more sensitive peptide bonds in the IgA backbone."}
{"id": "PMID:1090650", "title": "Increased immunologic reactivity between human glomerular basement membrane and group A type 12 streptococcal cell membrane after carbohydrase treatment.", "content": "Anti-streptococcal cell membrane and anti-human glomerular antisera were evaluated on normal human kidney sections by an indirect immunofluorescent test both before and after absorptions with homologous and heterologous membrane preparations. The observed cross-reactions were potentiated by removal of carbohydrate units from both membrane-types by carbohydrase, a mixture of carbohydrate-cleaving enzymes.", "contents": "Increased immunologic reactivity between human glomerular basement membrane and group A type 12 streptococcal cell membrane after carbohydrase treatment. Anti-streptococcal cell membrane and anti-human glomerular antisera were evaluated on normal human kidney sections by an indirect immunofluorescent test both before and after absorptions with homologous and heterologous membrane preparations. The observed cross-reactions were potentiated by removal of carbohydrate units from both membrane-types by carbohydrase, a mixture of carbohydrate-cleaving enzymes."}
{"id": "PMID:1090641", "title": "Selection of a resin system for anterior fracture treatment.", "content": "The tensile strength of the bond formed between etched bovine enamel and selected resin systems was evaluated; also, the need for a resin pulpal barrier was examined. Results indicate that the BIS-GMA unfilled primer is unnecessary for strength or as a pulpal protectant. The large scatter of results substantiates the clinical hypothesis that the effectiveness of the acid etch is highly variable.", "contents": "Selection of a resin system for anterior fracture treatment. The tensile strength of the bond formed between etched bovine enamel and selected resin systems was evaluated; also, the need for a resin pulpal barrier was examined. Results indicate that the BIS-GMA unfilled primer is unnecessary for strength or as a pulpal protectant. The large scatter of results substantiates the clinical hypothesis that the effectiveness of the acid etch is highly variable."}
{"id": "PMID:1090642", "title": "Scanning electron microscopy of dentition: methodology and ultrastructural morphology of tooth wear.", "content": "Scanning electron micrographs were taken of sets of human molars-those of paleo-Indians used in mastication of, ostensibly, a highly abrasive diet, and those of contemporary Americans. Different ultrastructural patterns of enamel wear were observed between the groups.", "contents": "Scanning electron microscopy of dentition: methodology and ultrastructural morphology of tooth wear. Scanning electron micrographs were taken of sets of human molars-those of paleo-Indians used in mastication of, ostensibly, a highly abrasive diet, and those of contemporary Americans. Different ultrastructural patterns of enamel wear were observed between the groups."}
{"id": "PMID:1090651", "title": "Blast transformation of rabbit B-derived lymphocytes by a mitogen extracted from Nocardia.", "content": "Nocardia water soluble mitogen (NWSM) is known to stimulate mouse and rabbit lymphoid cells and to act selectively on murine B-derived lymphocytes. In this paper, evidence is presented that NWSM is also a mitogen for rabbit bursal equivalent cells and does not bring about blast transformation of thymus-derived rabbit cells. Pretreatment of rabbit spleen lymphocytes with antibody directed against rabbit thymus lymphocyte antigen (anti RTLA serum) and with complement did not affect the strong increase of thymidine incorporation which follows stimulation with NWSM. The mitogen-induced polyclonal activation of antibody-forming cells and resulted in the presence of 30% of cells with the ultrastructural characteristics of plasmocytes. These observations led to the conclusion that NWSM is a mitogen for a B-derived lymphocyte of the rabbit.", "contents": "Blast transformation of rabbit B-derived lymphocytes by a mitogen extracted from Nocardia. Nocardia water soluble mitogen (NWSM) is known to stimulate mouse and rabbit lymphoid cells and to act selectively on murine B-derived lymphocytes. In this paper, evidence is presented that NWSM is also a mitogen for rabbit bursal equivalent cells and does not bring about blast transformation of thymus-derived rabbit cells. Pretreatment of rabbit spleen lymphocytes with antibody directed against rabbit thymus lymphocyte antigen (anti RTLA serum) and with complement did not affect the strong increase of thymidine incorporation which follows stimulation with NWSM. The mitogen-induced polyclonal activation of antibody-forming cells and resulted in the presence of 30% of cells with the ultrastructural characteristics of plasmocytes. These observations led to the conclusion that NWSM is a mitogen for a B-derived lymphocyte of the rabbit."}
{"id": "PMID:1090652", "title": "Migration inhibitory factor and type II interferon in the circulation of mice sensitized with mycobacterial components.", "content": "Migration inhibitory factor (MIF) and type II interferon were released into the circulation after mice had been sensitized i.v. with a bacillus of Calmette and Guerin (BCG) cell-wall-in-oil vaccine and then challenged four weeks later with 50 mg old tuberculin. At least two components of BCG, protein (PPD) and lipid (P-3), have been identified which are essential for the type of sensitization in which mediators are released after appropriate challenge. The route of sensitization had a marked effect on the development of delayed footpad reactions, release of lymphokines, and resistance to infection with virulent tubercle bacilli. Sensitization by the subcutaneous route tended to induce more pronounced delayed footpad reactions, whereas the i.v. route of sensitization was associated with maximum release of mediators. A close correlation existed between the conditions of sensitization that resulted in maximum production of lymphokines (MIF and Type II interferon) and those that caused protection against aerosol challenge with a virulent strain of Mycobacterium tuberculosis.", "contents": "Migration inhibitory factor and type II interferon in the circulation of mice sensitized with mycobacterial components. Migration inhibitory factor (MIF) and type II interferon were released into the circulation after mice had been sensitized i.v. with a bacillus of Calmette and Guerin (BCG) cell-wall-in-oil vaccine and then challenged four weeks later with 50 mg old tuberculin. At least two components of BCG, protein (PPD) and lipid (P-3), have been identified which are essential for the type of sensitization in which mediators are released after appropriate challenge. The route of sensitization had a marked effect on the development of delayed footpad reactions, release of lymphokines, and resistance to infection with virulent tubercle bacilli. Sensitization by the subcutaneous route tended to induce more pronounced delayed footpad reactions, whereas the i.v. route of sensitization was associated with maximum release of mediators. A close correlation existed between the conditions of sensitization that resulted in maximum production of lymphokines (MIF and Type II interferon) and those that caused protection against aerosol challenge with a virulent strain of Mycobacterium tuberculosis."}
{"id": "PMID:1090653", "title": "Stimulation of a T-independent primary anti-hapten response in vitro by TNP-lipopolysaccharide (TNP-LPS).", "content": "The trinitrophenyl hapten (TNP) has been covalently conjugated to bacterial lipopolysaccharides (LPS) to give TNP-LPS. The site of attachment has been suggested to be in the core polysaccharide and lipid A region of the molecule and approximately 2.4 hapten molecules are bound per monomer LPS molecule. The TNP-LPS has been demonstrated to be immunogenic in vitro at very low concentration. This antigen has further been shown to initiate a T-independent TNP-PFC response. The immunogenicity of TNP-LPS is abrogated by mild alkaline hydrolysis, suggesting a requirement for intact lipid A in the initiation of an immune respose at the very low concentrations of antigen used.", "contents": "Stimulation of a T-independent primary anti-hapten response in vitro by TNP-lipopolysaccharide (TNP-LPS). The trinitrophenyl hapten (TNP) has been covalently conjugated to bacterial lipopolysaccharides (LPS) to give TNP-LPS. The site of attachment has been suggested to be in the core polysaccharide and lipid A region of the molecule and approximately 2.4 hapten molecules are bound per monomer LPS molecule. The TNP-LPS has been demonstrated to be immunogenic in vitro at very low concentration. This antigen has further been shown to initiate a T-independent TNP-PFC response. The immunogenicity of TNP-LPS is abrogated by mild alkaline hydrolysis, suggesting a requirement for intact lipid A in the initiation of an immune respose at the very low concentrations of antigen used."}
{"id": "PMID:1090654", "title": "Effects of concanavalin A on the in vitro responses of mouse spleen cells to T-dependent and T-independent antigens.", "content": "?The stimulatory and inhibitory effects of concanavalin A (Con A) on the in vitro primary immune responses to a T-dependent antigen, sheep erythrocytes (SRBC) and a T-independent antigen, TNP-lipopolysaccharide (TNP-LPS) have been studied. Inhibition of the response to both antigens was optimal when 2 mug Con A were added at the initiation of the culture period. The response to SRBC was considerably enhanced by the addition of Con A 24 hr later. In contrast, this late addition did not stimulate the TNP-LPS response and often inhibited it. Inhibition of the TNP-LPS response required the participation of T cells since it was not observed in cells from adult thymectomized irradiated bone marrow-reconstituted (ATXBM) mice. The response to TNP-LPS was somewhat enhanced in ATXBM cells, but the degree of enhancement was strikingly less than that observed for SRBC. LPS per se did not block the stimulatory effect of Con A on the SRBC response, and was observed to act synergistically with this lectin. None of the Con A effects observed required the participation of adherent cells. These observations are consistent with a model in which different subpopulations of T cells are responsible for the inhibitory and stimulatory effects. They further suggest that the Con A inhibitory activity acts via a T cell to inhibit directly the B cell response to antigen.", "contents": "Effects of concanavalin A on the in vitro responses of mouse spleen cells to T-dependent and T-independent antigens. ?The stimulatory and inhibitory effects of concanavalin A (Con A) on the in vitro primary immune responses to a T-dependent antigen, sheep erythrocytes (SRBC) and a T-independent antigen, TNP-lipopolysaccharide (TNP-LPS) have been studied. Inhibition of the response to both antigens was optimal when 2 mug Con A were added at the initiation of the culture period. The response to SRBC was considerably enhanced by the addition of Con A 24 hr later. In contrast, this late addition did not stimulate the TNP-LPS response and often inhibited it. Inhibition of the TNP-LPS response required the participation of T cells since it was not observed in cells from adult thymectomized irradiated bone marrow-reconstituted (ATXBM) mice. The response to TNP-LPS was somewhat enhanced in ATXBM cells, but the degree of enhancement was strikingly less than that observed for SRBC. LPS per se did not block the stimulatory effect of Con A on the SRBC response, and was observed to act synergistically with this lectin. None of the Con A effects observed required the participation of adherent cells. These observations are consistent with a model in which different subpopulations of T cells are responsible for the inhibitory and stimulatory effects. They further suggest that the Con A inhibitory activity acts via a T cell to inhibit directly the B cell response to antigen."}
{"id": "PMID:1090655", "title": "Induction of theta-positive lymphocytes and lymphoblasts in mouse bone marrow by mitogens.", "content": "With a sensitive technique for the identification of lymphocytes and lymphoblasts bearing the surface antigens phi, TL, and immunoglobulin, the induction of phi-positive small lymphocytes in bone marrow could be observed. Dibutyryl cyclic adenosine 3',5'-monophosphate and thymosin were both effective in inducing precursor cells to express phi antigen. Concanavalin A and bacterial lipopolysaccharide were also shown to be inducers. After treatment with concanavalin A but not with lipopolysaccharide, lymphoblasts developed in marrow cultures which were positive for phi and TL antigens; when spleen was cultured with concanavalin A, lymphoblasts were phi-positive and TL negative. It is concluded that the cell responsive to concanavalin A (and phytohemagglutinin) in bone marrow is a phi-negative precursor (stem) cell, which is induced first to express phi and TL antigens, and then to differentiate to a lymphoblast.", "contents": "Induction of theta-positive lymphocytes and lymphoblasts in mouse bone marrow by mitogens. With a sensitive technique for the identification of lymphocytes and lymphoblasts bearing the surface antigens phi, TL, and immunoglobulin, the induction of phi-positive small lymphocytes in bone marrow could be observed. Dibutyryl cyclic adenosine 3',5'-monophosphate and thymosin were both effective in inducing precursor cells to express phi antigen. Concanavalin A and bacterial lipopolysaccharide were also shown to be inducers. After treatment with concanavalin A but not with lipopolysaccharide, lymphoblasts developed in marrow cultures which were positive for phi and TL antigens; when spleen was cultured with concanavalin A, lymphoblasts were phi-positive and TL negative. It is concluded that the cell responsive to concanavalin A (and phytohemagglutinin) in bone marrow is a phi-negative precursor (stem) cell, which is induced first to express phi and TL antigens, and then to differentiate to a lymphoblast."}
{"id": "PMID:1090656", "title": "Role of the clotting system in cell-mediated hypersensitivity. II. Kinetics of fibrinogen/fibrin accumulation and vascular permeability changes in tuberculin and cutaneous basophil hypersensitivity reactions.", "content": "Radioactive tracers and immunofluorescence were employed to detect and quantitate fibrinogen/fibrin deposition in two types of cell-mediated hypersensitivity reactions in the guinea pig. Classic delayed hypersensitivity (DH) reactions to Old Tuberculin and to the azobenzenearsonate hapten were characterized by a progressive increase in the fibrinogen (125-I-HF) content which exceeded that of the albumin tracer (131-I-HSA) and paralleled the development of induration and erythema. Accumulation of 125-I-HF could be related both to increased vascular permeability to 125-I-HF and, more specifically, to retarded efflux of extra vascular 125-I-HF from tuberculin reaction sites. Warfarin inhibited 125-I-HF accumulation and the formation of urea-insoluble 125-I-HF (cross-linked fibrin) as well as induration in tuberculin reactions. Immunofluorescence studies revealed the site of Fib deposition to be extravascular, among the connective tissue fibers of the dermis, similar to that in DH reactions in man. In contrast, little 125-I-HF accumulated in cell-mediated reactions rich in basophils--cutaneous basophil hypersensitivity (CBH) reactions to keyhole limpet hemocyanin, ovalbumin, and dinitrochlorobenzene--due in part to less vascular leakage of macromolecules and to decreased formation of urea-insoluble fibrin. By immunofluorescence Fib deposits were found in CBH reactions in a pattern similar to that in DH reactions, but the intensity of staining was appreciably less. Thus, fibrin accumulation further distinguishes DH from CBH reactions and is very likely responsible for the induration characteristic of DH reactions.", "contents": "Role of the clotting system in cell-mediated hypersensitivity. II. Kinetics of fibrinogen/fibrin accumulation and vascular permeability changes in tuberculin and cutaneous basophil hypersensitivity reactions. Radioactive tracers and immunofluorescence were employed to detect and quantitate fibrinogen/fibrin deposition in two types of cell-mediated hypersensitivity reactions in the guinea pig. Classic delayed hypersensitivity (DH) reactions to Old Tuberculin and to the azobenzenearsonate hapten were characterized by a progressive increase in the fibrinogen (125-I-HF) content which exceeded that of the albumin tracer (131-I-HSA) and paralleled the development of induration and erythema. Accumulation of 125-I-HF could be related both to increased vascular permeability to 125-I-HF and, more specifically, to retarded efflux of extra vascular 125-I-HF from tuberculin reaction sites. Warfarin inhibited 125-I-HF accumulation and the formation of urea-insoluble 125-I-HF (cross-linked fibrin) as well as induration in tuberculin reactions. Immunofluorescence studies revealed the site of Fib deposition to be extravascular, among the connective tissue fibers of the dermis, similar to that in DH reactions in man. In contrast, little 125-I-HF accumulated in cell-mediated reactions rich in basophils--cutaneous basophil hypersensitivity (CBH) reactions to keyhole limpet hemocyanin, ovalbumin, and dinitrochlorobenzene--due in part to less vascular leakage of macromolecules and to decreased formation of urea-insoluble fibrin. By immunofluorescence Fib deposits were found in CBH reactions in a pattern similar to that in DH reactions, but the intensity of staining was appreciably less. Thus, fibrin accumulation further distinguishes DH from CBH reactions and is very likely responsible for the induration characteristic of DH reactions."}
{"id": "PMID:1090657", "title": "Activation of guinea pig macrophages by bacterial lipopolysaccharide requires bone marrow-derived lymphocytes.", "content": "The activation of guinea pig peritoneal macrophages by Salmonella typhimurium lipopolysaccharide (LPS) was studied by using 14C-glucosamine uptake. Peritoneal exudate cells incorporated significant amounts of 14C-glucosamine when stimulated with LPS but neither purified macrophages nor nonadherent lymphocytes by themselves incorporated glucosamine. The activation of macrophages could be restored by adding nonadherent peritoneal lymphocytes, spleen cells, and lymph node cells but not thymocytes. Removal of B lymphocytes abolished the restorative capacity from active lymphoid cell populations. In contrast, B lymphocytes would restore glucosamine incorporation by macrophages stimulated with LPS but T lymphocytes did not. In addition, cell-free supernatants from LPS stimulated B lymphocytes but not from T lymphocytes could restore glucosamine incorporation by macrophages. These experiments demonstrate that LPS does not directly activate macrophages as measured by glucosamine incorporation but stimulates B lymphocytes which in turn activate macrophages.", "contents": "Activation of guinea pig macrophages by bacterial lipopolysaccharide requires bone marrow-derived lymphocytes. The activation of guinea pig peritoneal macrophages by Salmonella typhimurium lipopolysaccharide (LPS) was studied by using 14C-glucosamine uptake. Peritoneal exudate cells incorporated significant amounts of 14C-glucosamine when stimulated with LPS but neither purified macrophages nor nonadherent lymphocytes by themselves incorporated glucosamine. The activation of macrophages could be restored by adding nonadherent peritoneal lymphocytes, spleen cells, and lymph node cells but not thymocytes. Removal of B lymphocytes abolished the restorative capacity from active lymphoid cell populations. In contrast, B lymphocytes would restore glucosamine incorporation by macrophages stimulated with LPS but T lymphocytes did not. In addition, cell-free supernatants from LPS stimulated B lymphocytes but not from T lymphocytes could restore glucosamine incorporation by macrophages. These experiments demonstrate that LPS does not directly activate macrophages as measured by glucosamine incorporation but stimulates B lymphocytes which in turn activate macrophages."}
{"id": "PMID:1090658", "title": "The role of antibody feedback inhibition in the regulation of the secondary antibody response after high and low dose priming.", "content": "Spleen cells from mice immunized with a low dose of SRBC produce a greater secondary response to subsequent antigenic challenge in vivo than spleen cells from mice initially immunized with a larger dose. However, when the spleen cells are removed from the mice and given a secondary challange in tissue culture, a greater response is produced by cells from animals primed with the larger dose. Serum from the mice receiving the high dose of antigen contains low, but significant levels of specific antibody, and can inhibit further antibody production by lymphoid cells in vivo and in vitro. The characteristics of this suppression are similar to those known for passively administered specific antibody. Thus, antibody produced as a result of primary immunization may act through an inhibitory feedback mechanism in specifically limiting the magnitude of the secondary response to subsequent antigenic challenge in the normal animal", "contents": "The role of antibody feedback inhibition in the regulation of the secondary antibody response after high and low dose priming. Spleen cells from mice immunized with a low dose of SRBC produce a greater secondary response to subsequent antigenic challenge in vivo than spleen cells from mice initially immunized with a larger dose. However, when the spleen cells are removed from the mice and given a secondary challange in tissue culture, a greater response is produced by cells from animals primed with the larger dose. Serum from the mice receiving the high dose of antigen contains low, but significant levels of specific antibody, and can inhibit further antibody production by lymphoid cells in vivo and in vitro. The characteristics of this suppression are similar to those known for passively administered specific antibody. Thus, antibody produced as a result of primary immunization may act through an inhibitory feedback mechanism in specifically limiting the magnitude of the secondary response to subsequent antigenic challenge in the normal animal"}
{"id": "PMID:1090659", "title": "Rosette formation by canine peripheral blood lymphocytes.", "content": "The surface membrane characteristics of canine peripheral blood lymphocytes were investigated using erythrocyte (E) and erythrocyte antibody complement (EAC) rosette assays and immunofluorescent staining techniques. Canine thymus-derived lymphocytes formed nonimmune E rosettes with human and guinea pig erythrocytes at approximately the same percentages (mean percentage 36.3 and 32.4, respectively). Rosettes did not form with erythrocytes from seven other animal species. Cell surface immunoglobulins were demonstrable in a small percentage (4%) of the E rosette-forming lymphocyte population. Fragments of human erythrocytes inhibited E rosette formation by intact human red cells, but did not result in a significant decrease in rosette formation by intact guinea pig erythrocytes; likewise, guinea pig fragments had no inhibitory effect on rosette formation by human erythrocytes, demonstrating that separate receptors were required for the two red cell types. EAC rosette formation was not affected by addition of intact or fragmented human and guinea pig erythrocytes. Canine bone marrow-derived lymphocytes were characterized by immunoglobulin on the cell surface and EAC rosette formation. Serial tests of lymphocytes from one dog revealed a wide variation in percentage of cells forming E and EAC rosettes. A close correlation was observed between the immunoglobulin-bearing cells (mean percentage 46.6) and those forming EAC rosettes (mean percentage 49.3).", "contents": "Rosette formation by canine peripheral blood lymphocytes. The surface membrane characteristics of canine peripheral blood lymphocytes were investigated using erythrocyte (E) and erythrocyte antibody complement (EAC) rosette assays and immunofluorescent staining techniques. Canine thymus-derived lymphocytes formed nonimmune E rosettes with human and guinea pig erythrocytes at approximately the same percentages (mean percentage 36.3 and 32.4, respectively). Rosettes did not form with erythrocytes from seven other animal species. Cell surface immunoglobulins were demonstrable in a small percentage (4%) of the E rosette-forming lymphocyte population. Fragments of human erythrocytes inhibited E rosette formation by intact human red cells, but did not result in a significant decrease in rosette formation by intact guinea pig erythrocytes; likewise, guinea pig fragments had no inhibitory effect on rosette formation by human erythrocytes, demonstrating that separate receptors were required for the two red cell types. EAC rosette formation was not affected by addition of intact or fragmented human and guinea pig erythrocytes. Canine bone marrow-derived lymphocytes were characterized by immunoglobulin on the cell surface and EAC rosette formation. Serial tests of lymphocytes from one dog revealed a wide variation in percentage of cells forming E and EAC rosettes. A close correlation was observed between the immunoglobulin-bearing cells (mean percentage 46.6) and those forming EAC rosettes (mean percentage 49.3)."}
{"id": "PMID:1090660", "title": "Inhibition of the primary in vitro antibody response by interferon preparations.", "content": "The primary in vitro plaque forming cell (PFC) response of mouse (C57BL/6J) spleen cells to sheep red blood cells (SRBC) was inhibited by two sources of crude and two sources of partially purified mouse interferon. The substance(s) in the interferon preparations which induced PFC and antiviral inhibitory activity have the following common characteristics: a) The interferon preparations were of different potencies and specific activities, but they inhibited the PFC response in proportion to their activities; 20 to 60 units were the minimal amounts required for greater than 90% inhibition of the PFC response. b) Both the antiviral activity and the PFC inhibitory activity of the interferons were neutralized by antibody specific for mouse interferon. c) Both activities were partially or completely inactivated by heating at 60 degrees C for 1 h. d) Human interferons had neither antiviral activity nor PFC inhibitory activity in mouse cells. e) Limited exposure (4 h) of cells to interferon significantly inhibits both viral infection and the PFC response. f) Both the antiviral activity and the PFC inhibitory activity of the interferon preparations are acid stable. It is concluded, therefore, that the inhibition of the primary in vitro PFC response is due to interferon in the preparations. Maximum inhibition of the PFC response occurred when interferon was added to cultured at the same time as SRBC. Significant inhibition was also observed when interferon was added 1 day later, and slight enhancement was observed when added at days 2 and 3 of SRBC addition. Kinetic data showed that the greater the concentration of interferon added to the cultures, the earlier the effect on the PFC response. The presence of interferon in cultures for the first 4 h is sufficient to inhibit the PFC response. Interferon, then, appears to affect some early event(s), which leads to inhibition of the PFC response. Since mature T cells can be one of the sources of interferon, we have demonstrated a suppressor effect of a T cell lymphokine. The precise relationship of interferon to various expressions of suppressor T cell effects remains to be determined.", "contents": "Inhibition of the primary in vitro antibody response by interferon preparations. The primary in vitro plaque forming cell (PFC) response of mouse (C57BL/6J) spleen cells to sheep red blood cells (SRBC) was inhibited by two sources of crude and two sources of partially purified mouse interferon. The substance(s) in the interferon preparations which induced PFC and antiviral inhibitory activity have the following common characteristics: a) The interferon preparations were of different potencies and specific activities, but they inhibited the PFC response in proportion to their activities; 20 to 60 units were the minimal amounts required for greater than 90% inhibition of the PFC response. b) Both the antiviral activity and the PFC inhibitory activity of the interferons were neutralized by antibody specific for mouse interferon. c) Both activities were partially or completely inactivated by heating at 60 degrees C for 1 h. d) Human interferons had neither antiviral activity nor PFC inhibitory activity in mouse cells. e) Limited exposure (4 h) of cells to interferon significantly inhibits both viral infection and the PFC response. f) Both the antiviral activity and the PFC inhibitory activity of the interferon preparations are acid stable. It is concluded, therefore, that the inhibition of the primary in vitro PFC response is due to interferon in the preparations. Maximum inhibition of the PFC response occurred when interferon was added to cultured at the same time as SRBC. Significant inhibition was also observed when interferon was added 1 day later, and slight enhancement was observed when added at days 2 and 3 of SRBC addition. Kinetic data showed that the greater the concentration of interferon added to the cultures, the earlier the effect on the PFC response. The presence of interferon in cultures for the first 4 h is sufficient to inhibit the PFC response. Interferon, then, appears to affect some early event(s), which leads to inhibition of the PFC response. Since mature T cells can be one of the sources of interferon, we have demonstrated a suppressor effect of a T cell lymphokine. The precise relationship of interferon to various expressions of suppressor T cell effects remains to be determined."}
{"id": "PMID:1090661", "title": "Effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. I. Depression of the afferent arc.", "content": "Bacterial lipopolysaccharide (LPS), being a B cell mitogen, enhances antibody production. When given prior to an injection of sheep red blood cells (SRBC) it caused a premature but not significantly increased production of antibody forming cells in regionally stimulated lymph nodes. There was a corresponding depression of T cell activity, as measured by the relative levels of delayed-type hypersensitivity (DTH) in normal and LPS-treated mice. Although LPS also induced hyperplasia of the reticuloendothelial system, and a consequent reduction in the effective antigenic dose of SRBC, this was not the reason for the reduced level of T cell activity. The opposing effects of LPS on T and B cell responses was due to premature inhibition of the T cell response by an accelerated production of antibody. This caused an earlier onset of the inhibitory mechanism which regulates the T cell response and is specific for the inducing antigen.", "contents": "Effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. I. Depression of the afferent arc. Bacterial lipopolysaccharide (LPS), being a B cell mitogen, enhances antibody production. When given prior to an injection of sheep red blood cells (SRBC) it caused a premature but not significantly increased production of antibody forming cells in regionally stimulated lymph nodes. There was a corresponding depression of T cell activity, as measured by the relative levels of delayed-type hypersensitivity (DTH) in normal and LPS-treated mice. Although LPS also induced hyperplasia of the reticuloendothelial system, and a consequent reduction in the effective antigenic dose of SRBC, this was not the reason for the reduced level of T cell activity. The opposing effects of LPS on T and B cell responses was due to premature inhibition of the T cell response by an accelerated production of antibody. This caused an earlier onset of the inhibitory mechanism which regulates the T cell response and is specific for the inducing antigen."}
{"id": "PMID:1090662", "title": "Thymic regeneration after lethal irradiation evidence for an intra-thymic radioresistant T cell precursor.", "content": "The data presented indicate the existence of a significant pool of radioresistant stem cells which are capable of partially restoring the thymus of heavily irradiated mice. 3-H-TdR incorporation by the thymus of lethally irradiated mice begins 48 to 72 hr after irradiation and increases throughout the next 8 days. By the 9th day after 760 rads, typical corticomedullary architecture has been restored. 890 rads markedly suppressed, but did not totally eliminate this regeneration. Injection of large numbers of syngeneic bone marrow cells immediately after irradiation was without effect on the rate or extent of regeneration. Mice whose bone marrow and spleen were shielded from irradiation showed an identical amount of thymic regeneration as those receiving total body irradiation indicating that the precursor cell pool responsible for the early post irradiation phase of thymic regeneration is most likely an intrathymic population. The cells repopulating the thymus were morphologically indistinguishable from normal thymocytes and were susceptible to cytotoxic antisera against the thymic differentiation antigens Thy-1, TL, LyA2 and LyC2.", "contents": "Thymic regeneration after lethal irradiation evidence for an intra-thymic radioresistant T cell precursor. The data presented indicate the existence of a significant pool of radioresistant stem cells which are capable of partially restoring the thymus of heavily irradiated mice. 3-H-TdR incorporation by the thymus of lethally irradiated mice begins 48 to 72 hr after irradiation and increases throughout the next 8 days. By the 9th day after 760 rads, typical corticomedullary architecture has been restored. 890 rads markedly suppressed, but did not totally eliminate this regeneration. Injection of large numbers of syngeneic bone marrow cells immediately after irradiation was without effect on the rate or extent of regeneration. Mice whose bone marrow and spleen were shielded from irradiation showed an identical amount of thymic regeneration as those receiving total body irradiation indicating that the precursor cell pool responsible for the early post irradiation phase of thymic regeneration is most likely an intrathymic population. The cells repopulating the thymus were morphologically indistinguishable from normal thymocytes and were susceptible to cytotoxic antisera against the thymic differentiation antigens Thy-1, TL, LyA2 and LyC2."}
{"id": "PMID:1090663", "title": "Immunoglobulin D-bearing lymphocytes in primary immunodeficiencies.", "content": "Surface IgD on blood lymphocytes was studied in 10 normal adults and 24 patients with primary immunodeficiencies by direct immunofluorescence, together with surface immunoglobulins of the other classes and with spontaneous rosette formation with sheep erythrocytes. In the normal adults, 8% of the lymphocytes bore delta chains (the figures for mu chains being 11%) and, among the cells positive for mu and/or delta, 70% were mixed stained, 22% and 8% being single stained for mu and delta respectively. In 10 patients with sex-linked agammaglobulinemia or variable immuno-deficiency, practically no cells bearing surface immunoglobulins, including IgD, were detectable. A normal distribution of surface immunoglobulins, including the results of double labeling for mu and delta, was found in five other immunodeficiency patients in whom there was a block of the terminal differentiation of B lymphocytes into plasma cells. A new kind of block in the differentiation of the B cell line was observed in two patients affected with sex-linked severe combined immunodeficiency and variable immuno-deficiency respectively. They showed high figures for IgD-bearing lymphocytes, some of which carried simultaneously mu chains, contrasting with the absence of lymphocytes carrying IgM without IgD and of IgG- or IgA-bearing cells. The data obtained in several other patients with low figures for IgG- and IgA-bearing lymphocytes and a predominance of IgD-carrying cells with an excess of single producers for delta chains over single producers for mu chains suggest an analogous but incomplete maturation arrest.", "contents": "Immunoglobulin D-bearing lymphocytes in primary immunodeficiencies. Surface IgD on blood lymphocytes was studied in 10 normal adults and 24 patients with primary immunodeficiencies by direct immunofluorescence, together with surface immunoglobulins of the other classes and with spontaneous rosette formation with sheep erythrocytes. In the normal adults, 8% of the lymphocytes bore delta chains (the figures for mu chains being 11%) and, among the cells positive for mu and/or delta, 70% were mixed stained, 22% and 8% being single stained for mu and delta respectively. In 10 patients with sex-linked agammaglobulinemia or variable immuno-deficiency, practically no cells bearing surface immunoglobulins, including IgD, were detectable. A normal distribution of surface immunoglobulins, including the results of double labeling for mu and delta, was found in five other immunodeficiency patients in whom there was a block of the terminal differentiation of B lymphocytes into plasma cells. A new kind of block in the differentiation of the B cell line was observed in two patients affected with sex-linked severe combined immunodeficiency and variable immuno-deficiency respectively. They showed high figures for IgD-bearing lymphocytes, some of which carried simultaneously mu chains, contrasting with the absence of lymphocytes carrying IgM without IgD and of IgG- or IgA-bearing cells. The data obtained in several other patients with low figures for IgG- and IgA-bearing lymphocytes and a predominance of IgD-carrying cells with an excess of single producers for delta chains over single producers for mu chains suggest an analogous but incomplete maturation arrest."}
{"id": "PMID:1090664", "title": "Enhancement of nitroblue tetrazolium dye reduction by leukocytes exposed to a component of complement in the absence of phagocytosis.", "content": "Enhancement of in vitro nitroblue tetrazolium dye reduction by human polymorphonuclear leukocytes may be mediated by a low molecular weight fluid phase component of the complement system. This occurs in the absence of phagocytosis and is associated with an increase in leukocyte hexose monophosphate shunt activity. The stimulatory component may be generated by activating the alternate complement pathway in serum, and shares many of the properties of human C5a. Thus, the enhanced spontaneous reduction of nitroblue tetrazolium by leukocytes from patients with bacterial sepsis may not necessarily be due to the phagocytic activity of these cells, but rather may occur as a consequence of in vivo complement activation by either intact microorganisms or their products.", "contents": "Enhancement of nitroblue tetrazolium dye reduction by leukocytes exposed to a component of complement in the absence of phagocytosis. Enhancement of in vitro nitroblue tetrazolium dye reduction by human polymorphonuclear leukocytes may be mediated by a low molecular weight fluid phase component of the complement system. This occurs in the absence of phagocytosis and is associated with an increase in leukocyte hexose monophosphate shunt activity. The stimulatory component may be generated by activating the alternate complement pathway in serum, and shares many of the properties of human C5a. Thus, the enhanced spontaneous reduction of nitroblue tetrazolium by leukocytes from patients with bacterial sepsis may not necessarily be due to the phagocytic activity of these cells, but rather may occur as a consequence of in vivo complement activation by either intact microorganisms or their products."}
{"id": "PMID:1090665", "title": "Effect of cortisone on the disappearance kinetics and tissue localization of soluble immune complexes.", "content": "The effect of cortisone treatment on the disappearance kinetics, the hepatic uptake, and the glomerular deposition of i.v. administered, soluble immune complexes (HSAantiHSA) was examined in mice. An initial rapid disappearance of complexes from the circulation occurred after the injection of complexes into control mice, which was caused by increased vascular permeability. This phase was absent in the cortisone-treated group. The half life of complexes composed of more than two antigen and two antibody molecules (greater than Ag2Ab2)was prolonged from 1.93 hr in control mice to 4.71 hr in cortisone-treated mice while the half life of Ag2Ab2 complexes was unchanged (11.40 hr vs 12.04 hr). Although the clearance velocity of greater than Ag2Ab2 complexes was suppressed in cortisone-treated mice, the quantity of complexes specifically located in the liver at 1, 2, and 4 hr was not significantly different in the two groups. Persistence of circulating greater than Ag1Ab2 complexes was associated with enhanced and prolonged glomerular depostion of complexes in the cortisone-treated mice.", "contents": "Effect of cortisone on the disappearance kinetics and tissue localization of soluble immune complexes. The effect of cortisone treatment on the disappearance kinetics, the hepatic uptake, and the glomerular deposition of i.v. administered, soluble immune complexes (HSAantiHSA) was examined in mice. An initial rapid disappearance of complexes from the circulation occurred after the injection of complexes into control mice, which was caused by increased vascular permeability. This phase was absent in the cortisone-treated group. The half life of complexes composed of more than two antigen and two antibody molecules (greater than Ag2Ab2)was prolonged from 1.93 hr in control mice to 4.71 hr in cortisone-treated mice while the half life of Ag2Ab2 complexes was unchanged (11.40 hr vs 12.04 hr). Although the clearance velocity of greater than Ag2Ab2 complexes was suppressed in cortisone-treated mice, the quantity of complexes specifically located in the liver at 1, 2, and 4 hr was not significantly different in the two groups. Persistence of circulating greater than Ag1Ab2 complexes was associated with enhanced and prolonged glomerular depostion of complexes in the cortisone-treated mice."}
{"id": "PMID:1090666", "title": "Partial characterization of leukocyte inhibitory factor by concanavalin A-stimulated human lymphocytes (LIF Con A).", "content": "Leukocyte inhibitory factor (LIF) obtained from normal human lymphocytes stimulated by Concanavalin A was characterized by Sephadex gel filtration, disc electrophoresis, isopycnic centrifugation, enzymatic treatment and by immunoabsorbent studies. LIF activity was found after Sephadex G-100 gel filtration in a fraction containing molecules having the size of albumin (68,000 daltons). This is the same region were LIF activity derived from antigen-stimulated lymphocytes has previously been found to elute. On disc gel electrophoresis at pH 9.1, peak LIF activity was eluted from the acrylamide gel fraction containing molecules that migrate with albumin. In isopycnic centrifugation studies, human LIF was shown to have a buoyant density similar to that of pure protein. Human LIF was inactivated by treatment with chymotrypsin but not by neuraminidase treatment. LIF activity could not be removed by incubating the material on columns containing Sepharose 6b coupled with anti-human serum albumin or anti-Fab. The characteristics of human LIF and migration inhibitory factor were compared.", "contents": "Partial characterization of leukocyte inhibitory factor by concanavalin A-stimulated human lymphocytes (LIF Con A). Leukocyte inhibitory factor (LIF) obtained from normal human lymphocytes stimulated by Concanavalin A was characterized by Sephadex gel filtration, disc electrophoresis, isopycnic centrifugation, enzymatic treatment and by immunoabsorbent studies. LIF activity was found after Sephadex G-100 gel filtration in a fraction containing molecules having the size of albumin (68,000 daltons). This is the same region were LIF activity derived from antigen-stimulated lymphocytes has previously been found to elute. On disc gel electrophoresis at pH 9.1, peak LIF activity was eluted from the acrylamide gel fraction containing molecules that migrate with albumin. In isopycnic centrifugation studies, human LIF was shown to have a buoyant density similar to that of pure protein. Human LIF was inactivated by treatment with chymotrypsin but not by neuraminidase treatment. LIF activity could not be removed by incubating the material on columns containing Sepharose 6b coupled with anti-human serum albumin or anti-Fab. The characteristics of human LIF and migration inhibitory factor were compared."}
{"id": "PMID:1090667", "title": "Non-random distribution of surface immunoglobulins on murine B lymphocytes.", "content": "An indirect labeling technique was used to map the surface immunoglobulin (Ig) on murine splenic B lymphocytes by freeze-etching. Cells were labeled first with fluorescein conjugates of monovalent (papain-digested) anti-Ig antibody followed by monovalent anti-fluorescein antibody coupled to ferritin. The technique avoids cross-linking and aggregation of surface Ig. Freeze-etched replicas of cells labeled at 4 degrees C, as well as of cells prefixed with paraformaldehyde showed that surface Ig was distributed in small clusters with interconnecting networks. The observed pattern was analyzed statistically by comparing it with the expected random (Poisson) distribution and shown to be non-random to a high degree of statistical significance. The deviation from randomness could be explained by the presence of clusters and relative excesses of bare membrane. Such an observed distribution of surface Ig suggests that this membrane macromolecule may be organized in a specific manner. The distribution may also play a role in the function of surface Ig as the antigen receptor on B lymphocytes.", "contents": "Non-random distribution of surface immunoglobulins on murine B lymphocytes. An indirect labeling technique was used to map the surface immunoglobulin (Ig) on murine splenic B lymphocytes by freeze-etching. Cells were labeled first with fluorescein conjugates of monovalent (papain-digested) anti-Ig antibody followed by monovalent anti-fluorescein antibody coupled to ferritin. The technique avoids cross-linking and aggregation of surface Ig. Freeze-etched replicas of cells labeled at 4 degrees C, as well as of cells prefixed with paraformaldehyde showed that surface Ig was distributed in small clusters with interconnecting networks. The observed pattern was analyzed statistically by comparing it with the expected random (Poisson) distribution and shown to be non-random to a high degree of statistical significance. The deviation from randomness could be explained by the presence of clusters and relative excesses of bare membrane. Such an observed distribution of surface Ig suggests that this membrane macromolecule may be organized in a specific manner. The distribution may also play a role in the function of surface Ig as the antigen receptor on B lymphocytes."}
{"id": "PMID:1090668", "title": "Capping of the lymphocyte C3 receptor and temperature-dependent loss of C3 rosettes.", "content": "The percentage of mouse spleen cells rosetted by bacteria-antibody-complement complexes (BAC) was found to be temperature dependent. Maximal rosetting was observed when BAC and spleen cells were incubated in ice (0.5 degrees C), whereas less rosetting resulted from 37 degrees C incubations. When rosetted cells were incubated at 37 degrees C, cap formation was found to occur, and indirect evidence suggested that the capped bacteria were subsequently shed from the lymphocyte surface. Neither capping nor shedding occurred at 0.5 degrees C, suggesting that these phenomena were responsible for the decreased percentage of rosetted cells found after incubation at 37 degrees C. An incidental observation indicated that a second factor potentially contributing to the loss of rosetted cells at 37 degrees C was the removal of BAC from the surface of cells by phagocytes.", "contents": "Capping of the lymphocyte C3 receptor and temperature-dependent loss of C3 rosettes. The percentage of mouse spleen cells rosetted by bacteria-antibody-complement complexes (BAC) was found to be temperature dependent. Maximal rosetting was observed when BAC and spleen cells were incubated in ice (0.5 degrees C), whereas less rosetting resulted from 37 degrees C incubations. When rosetted cells were incubated at 37 degrees C, cap formation was found to occur, and indirect evidence suggested that the capped bacteria were subsequently shed from the lymphocyte surface. Neither capping nor shedding occurred at 0.5 degrees C, suggesting that these phenomena were responsible for the decreased percentage of rosetted cells found after incubation at 37 degrees C. An incidental observation indicated that a second factor potentially contributing to the loss of rosetted cells at 37 degrees C was the removal of BAC from the surface of cells by phagocytes."}
{"id": "PMID:1090669", "title": "Electrophoretic fractionation of guniea pig lymphocytes: evidence for different subsets of T and B cells in spleen and lymph node.", "content": "Guinea pig lymph node and blood lymphocytes have been physically fractionated in preparative cell electrophoresis into two functionally viable populations, the high mobility cell population (HMC) and the low mobility cell population (LMC). By using cell surface markers and functional tests known to be specific for T and B lymphocytes, respectively, it is shown that the T lymphocytes localize in the HMC population and the B lymphocytes in the LMC population. The spleen lymphocytes do not separate into the two populations. They move into one single broad peak containing both T and B cells. This finding indicates the presence of electrokinetically different subsets of T and B lymphocytes in the spleen on one hand and in the lymph node and blood on the other hand.", "contents": "Electrophoretic fractionation of guniea pig lymphocytes: evidence for different subsets of T and B cells in spleen and lymph node. Guinea pig lymph node and blood lymphocytes have been physically fractionated in preparative cell electrophoresis into two functionally viable populations, the high mobility cell population (HMC) and the low mobility cell population (LMC). By using cell surface markers and functional tests known to be specific for T and B lymphocytes, respectively, it is shown that the T lymphocytes localize in the HMC population and the B lymphocytes in the LMC population. The spleen lymphocytes do not separate into the two populations. They move into one single broad peak containing both T and B cells. This finding indicates the presence of electrokinetically different subsets of T and B lymphocytes in the spleen on one hand and in the lymph node and blood on the other hand."}
{"id": "PMID:1090670", "title": "Humoral immunity in rodent malaria. III: Studies on the site of antibody action.", "content": "Serum which protects rats against Plasmodium berghei infections fails to sensitize parasitized erythrocytes in vitro for in vivo destruction. Further, the efflux of 86Rb from parasitized erythrocytes in the presence of complement is not accelerated. On administration to animals with preexisting malaria, it does, however, produce a relatively slow decline in parasitemia, a phenomenon interpreted in terms of the gradual production and/or release of reactive antigenic determinants associated with maturation of the parasites. Protective activity is elaborated in the serum of animals during the development of parasitemia, thus creating a situation in which there is apparently the coincident presence of antibody and its putative antigen in the circulation. Little absorption of protective activity by parasitized cells or parasites was observed in vitro. However,  in vivo removal of the protective activity during an ongoing infection could be demonstrated. These observations favor the notion that protective antibody exerts its influence not on the bulk of the parasitized erythrocytes, but rather on some subpopulation thereof. The cumulative evidence of this and other studies suggests that the stage(s) involved are the schizonts and/or merozoites.", "contents": "Humoral immunity in rodent malaria. III: Studies on the site of antibody action. Serum which protects rats against Plasmodium berghei infections fails to sensitize parasitized erythrocytes in vitro for in vivo destruction. Further, the efflux of 86Rb from parasitized erythrocytes in the presence of complement is not accelerated. On administration to animals with preexisting malaria, it does, however, produce a relatively slow decline in parasitemia, a phenomenon interpreted in terms of the gradual production and/or release of reactive antigenic determinants associated with maturation of the parasites. Protective activity is elaborated in the serum of animals during the development of parasitemia, thus creating a situation in which there is apparently the coincident presence of antibody and its putative antigen in the circulation. Little absorption of protective activity by parasitized cells or parasites was observed in vitro. However,  in vivo removal of the protective activity during an ongoing infection could be demonstrated. These observations favor the notion that protective antibody exerts its influence not on the bulk of the parasitized erythrocytes, but rather on some subpopulation thereof. The cumulative evidence of this and other studies suggests that the stage(s) involved are the schizonts and/or merozoites."}
{"id": "PMID:1090671", "title": "Activation of T and B lymphocytes in vitro. IV. Regulatory influence on specific T cell functions by a thymus extract factor.", "content": "In the presence of a preparation of purified thymus extract factors (TEF), spleen cells from athymic nude mice develop in vitro immune responses to soluble DNP-protein conjugates, to particulate sheep red blood cells, and to allogeneic cells. TEF has similar effects also on the in vitro antibody responses of normal mouse spleen cells, enhancing antigen-dependent plaque-forming cells responses to dinitrophenyl carrier complexes of unprimed and primed mice. TEF fails to reconstitute helper cell function in cultures of T cell-deprived spleen cells. The thymic factor increases the reactivity or normal thymus cells in the in vitro mixed lymphocyte reaction and has at high doses mitogenic effects. The role of humoral thymus factors on the differentiation process of T cells is discussed.", "contents": "Activation of T and B lymphocytes in vitro. IV. Regulatory influence on specific T cell functions by a thymus extract factor. In the presence of a preparation of purified thymus extract factors (TEF), spleen cells from athymic nude mice develop in vitro immune responses to soluble DNP-protein conjugates, to particulate sheep red blood cells, and to allogeneic cells. TEF has similar effects also on the in vitro antibody responses of normal mouse spleen cells, enhancing antigen-dependent plaque-forming cells responses to dinitrophenyl carrier complexes of unprimed and primed mice. TEF fails to reconstitute helper cell function in cultures of T cell-deprived spleen cells. The thymic factor increases the reactivity or normal thymus cells in the in vitro mixed lymphocyte reaction and has at high doses mitogenic effects. The role of humoral thymus factors on the differentiation process of T cells is discussed."}
{"id": "PMID:1090672", "title": "The role of macrophages in the production of lymphokines by T and B lymphocytes.", "content": "The role of macrophages in the production of two lymphokines, monocyte chemotactic factor and macrophage activating factor, was investigated. Lymphokine production by guinea pig lymph node and spleen cells required macrophages for thymus-dependent antigens and mitogens. In contrast, B cell stimulants which also induce the synthesis of lymphokines were macrophage independent. When populations of relatively pure B or T lymphocytes were isolated, it was found that T cells required viable macrophage cooperation to produce these two lymphokines and to undergo propliferation in response to specific antigens, whereas B cells could be directly activated in the absence of macrophages. These findings suggest that T and B cells have different requirements for activation and for macrophage cooperation. Futhermore, since lymphokine synthesis is evident within the first 4 hr of stimulant presentation, these observations demonstrate that macrophages play an essential role in the earliest events of lymphocyte activation.", "contents": "The role of macrophages in the production of lymphokines by T and B lymphocytes. The role of macrophages in the production of two lymphokines, monocyte chemotactic factor and macrophage activating factor, was investigated. Lymphokine production by guinea pig lymph node and spleen cells required macrophages for thymus-dependent antigens and mitogens. In contrast, B cell stimulants which also induce the synthesis of lymphokines were macrophage independent. When populations of relatively pure B or T lymphocytes were isolated, it was found that T cells required viable macrophage cooperation to produce these two lymphokines and to undergo propliferation in response to specific antigens, whereas B cells could be directly activated in the absence of macrophages. These findings suggest that T and B cells have different requirements for activation and for macrophage cooperation. Futhermore, since lymphokine synthesis is evident within the first 4 hr of stimulant presentation, these observations demonstrate that macrophages play an essential role in the earliest events of lymphocyte activation."}
{"id": "PMID:1090673", "title": "Regulation by antibody of phytolectin induced lymphocyte proliferation. I. Evidence for two mechanisms of suppression.", "content": "Studies were conducted to determine the mechanisms of antibody suppression of phytohemagglutinin (PHA)- and concanavalin A (Con A)-induced lymphocyte proliferation. Initial experiments incubated lymphocytes with either PHA or Con A, and at various times the respective anti-phytolectin was added to the cultures. DNA synthesis was less than 20% of the total response if anti-PHA was added within 2 hr and if anti-Con A was added within 10 hr of addition of mitogens to the culture. At the concentrations used, anti-PHA reduced PHA binding to the lymphocytes, while anti-Con A did not reduce Con A binding to the cells. Antibody was non-toxic and specific for its respective mitogen. Additional experiments incubated lymphocytes with either PHA or Con A and then the cells were washed and placed in mitogen-free media. The addition of antibody to cultures which had been washed markedly suppressed proliferation. Using radiolabeled PHA and Con A in one group of experiments and fluorescent labeled anti-mitogen antibodies in others, it was determined that antibody did not remove Con A or PHA from the cell, but instead antibody slowed the release of mitogen from the lymphocyte. Anti-mitogen antibody remained attached to mitogen on the surface of lymphocytes for at least 24 hr. The experiments suggest that antibody can suppress the lymphocyte reaction by blocking the necessary phytolectinlymphocyte interaction and interrupting stimulation by cell-bound mitogen.", "contents": "Regulation by antibody of phytolectin induced lymphocyte proliferation. I. Evidence for two mechanisms of suppression. Studies were conducted to determine the mechanisms of antibody suppression of phytohemagglutinin (PHA)- and concanavalin A (Con A)-induced lymphocyte proliferation. Initial experiments incubated lymphocytes with either PHA or Con A, and at various times the respective anti-phytolectin was added to the cultures. DNA synthesis was less than 20% of the total response if anti-PHA was added within 2 hr and if anti-Con A was added within 10 hr of addition of mitogens to the culture. At the concentrations used, anti-PHA reduced PHA binding to the lymphocytes, while anti-Con A did not reduce Con A binding to the cells. Antibody was non-toxic and specific for its respective mitogen. Additional experiments incubated lymphocytes with either PHA or Con A and then the cells were washed and placed in mitogen-free media. The addition of antibody to cultures which had been washed markedly suppressed proliferation. Using radiolabeled PHA and Con A in one group of experiments and fluorescent labeled anti-mitogen antibodies in others, it was determined that antibody did not remove Con A or PHA from the cell, but instead antibody slowed the release of mitogen from the lymphocyte. Anti-mitogen antibody remained attached to mitogen on the surface of lymphocytes for at least 24 hr. The experiments suggest that antibody can suppress the lymphocyte reaction by blocking the necessary phytolectinlymphocyte interaction and interrupting stimulation by cell-bound mitogen."}
{"id": "PMID:1090674", "title": "Normal function of immunologic stem cells from aged mice.", "content": "Marrow or spleen grafts from aged donor mice produced antibody-forming cells as effectively as did grafts from younger controls in recipients tested 3 to 10 months after the transplantation. All recipients were lethally irradiated, and the T6 chromosome marker was used to demonstrate that they were populated by donor cell lines. Recipients of aged or younger control grafts gave similar responses when stimulated with varying doses of antigen and when tested at different times after the transplantation except in two cases: 1) Recipients of aged spleen grafts gave significantly lower responses than younger controls for the first few weeks after the transplantation. 2) If recipients had been thymectomized before lethal irradiation, aged cell lines (pooled marrow and spleen cells) gave only 37% of the responses of younger controls. Given sufficient time and intact young recipients, immunologic stem cell lines from old donors populated recipients with cells having normal immune responses. These results suggest that age-related immunologic defects are not intrinisically timed in the precursor cell lines that populate the immune system.", "contents": "Normal function of immunologic stem cells from aged mice. Marrow or spleen grafts from aged donor mice produced antibody-forming cells as effectively as did grafts from younger controls in recipients tested 3 to 10 months after the transplantation. All recipients were lethally irradiated, and the T6 chromosome marker was used to demonstrate that they were populated by donor cell lines. Recipients of aged or younger control grafts gave similar responses when stimulated with varying doses of antigen and when tested at different times after the transplantation except in two cases: 1) Recipients of aged spleen grafts gave significantly lower responses than younger controls for the first few weeks after the transplantation. 2) If recipients had been thymectomized before lethal irradiation, aged cell lines (pooled marrow and spleen cells) gave only 37% of the responses of younger controls. Given sufficient time and intact young recipients, immunologic stem cell lines from old donors populated recipients with cells having normal immune responses. These results suggest that age-related immunologic defects are not intrinisically timed in the precursor cell lines that populate the immune system."}
{"id": "PMID:1090675", "title": "Antibody production and interaction with lymphoid cells in relation to tumor immunity in the Moloney sarcoma virus system.", "content": "The temporal development of antibodies to Moloney sarcoma virus (MSV)-induced antigens in relation to tumor progression was followed with the membrane immunofluorescence (MF) and antibody-dependent normal lymphocyte cytotoxicity (ADNLC) assays. Antibody was detected at 14 days following virus infection by MF. In mice that developed primary tumors which regressed, MF titers developed to high levels following a period between 2 and 4 weeks post-innoculation during which the titers remained at low and constant levels. The increase in MF titers corresponded closely on a temporal basis to the initiation of the regression process. In contrast, antibody remained at low levels in those mice that developed progressively growing tumors. ADNLC was  detected in the sera of regressor mice approximately 6 weeks post-inoculation but was not detectable at any of the time interavals in sera from mice with progressively growing tumors. Attempts to enhance the cytotoxic activity of normal spleen cells directly with immune serum were unsuccessful. The synergistic effect of serum on the cytotoxic activity of immune lymphocytes was less pronounced than with normal lymphocytes in this system. Temporal studies  indicated that there was an inverse relationship between the development of cellular immunity and the capablility of these cells to be activated in the ADNLC assay.", "contents": "Antibody production and interaction with lymphoid cells in relation to tumor immunity in the Moloney sarcoma virus system. The temporal development of antibodies to Moloney sarcoma virus (MSV)-induced antigens in relation to tumor progression was followed with the membrane immunofluorescence (MF) and antibody-dependent normal lymphocyte cytotoxicity (ADNLC) assays. Antibody was detected at 14 days following virus infection by MF. In mice that developed primary tumors which regressed, MF titers developed to high levels following a period between 2 and 4 weeks post-innoculation during which the titers remained at low and constant levels. The increase in MF titers corresponded closely on a temporal basis to the initiation of the regression process. In contrast, antibody remained at low levels in those mice that developed progressively growing tumors. ADNLC was  detected in the sera of regressor mice approximately 6 weeks post-inoculation but was not detectable at any of the time interavals in sera from mice with progressively growing tumors. Attempts to enhance the cytotoxic activity of normal spleen cells directly with immune serum were unsuccessful. The synergistic effect of serum on the cytotoxic activity of immune lymphocytes was less pronounced than with normal lymphocytes in this system. Temporal studies  indicated that there was an inverse relationship between the development of cellular immunity and the capablility of these cells to be activated in the ADNLC assay."}
{"id": "PMID:1090676", "title": "Antibody affinity. VIII. Measurement of affinity of anti-lactose antibody by fluorescence quenching with a DNP-containing ligand.", "content": "The method of fluorescence quenching by bound ligand for the measurement of antibody affinity has been extended to anti-lactose antibodies. The basis for this extension is the use of a lactosyl ligand covalently linked to the 2,4-dinitrophenyl (DNP) group. This group serves as a sensor of the bound state by causing fluorescence quenching similar to that observed with anti-DNP antibody. The synthesis and characterization of this new ligand, N-(Nalpha-acetyl,Nepsilon-DNP-L-lysyl)-rho-aminophenyl-beta-lactoside, are described. The affinity of its interaction with three rabbit IgG and one equine IgM antilactose antibody preparations has been measured. The Qmax values range from 24 to 47% and the calculated association constants from 2.5 times 10-5 M-1 to 7.2 times 10-5 M-1. For one IgG preparation, measurement of the association constant by equilibrium dialysis with the tritiated ligand gave a value of 6.75 times 10-5 M-1 compared to the value of 7.2 times 10-5 M-1 obtained by fluorescence quenching. It is evident that with monoclonal anti-lactose antibody the accurate determination of Qmax, which is possible in this system, provides another structurally dependent clonal characterization of the variable region of the antibody. This optical probe along with others can be used for the identification of individual clones of antibody-producing cells through time and inheritance.", "contents": "Antibody affinity. VIII. Measurement of affinity of anti-lactose antibody by fluorescence quenching with a DNP-containing ligand. The method of fluorescence quenching by bound ligand for the measurement of antibody affinity has been extended to anti-lactose antibodies. The basis for this extension is the use of a lactosyl ligand covalently linked to the 2,4-dinitrophenyl (DNP) group. This group serves as a sensor of the bound state by causing fluorescence quenching similar to that observed with anti-DNP antibody. The synthesis and characterization of this new ligand, N-(Nalpha-acetyl,Nepsilon-DNP-L-lysyl)-rho-aminophenyl-beta-lactoside, are described. The affinity of its interaction with three rabbit IgG and one equine IgM antilactose antibody preparations has been measured. The Qmax values range from 24 to 47% and the calculated association constants from 2.5 times 10-5 M-1 to 7.2 times 10-5 M-1. For one IgG preparation, measurement of the association constant by equilibrium dialysis with the tritiated ligand gave a value of 6.75 times 10-5 M-1 compared to the value of 7.2 times 10-5 M-1 obtained by fluorescence quenching. It is evident that with monoclonal anti-lactose antibody the accurate determination of Qmax, which is possible in this system, provides another structurally dependent clonal characterization of the variable region of the antibody. This optical probe along with others can be used for the identification of individual clones of antibody-producing cells through time and inheritance."}
{"id": "PMID:1090678", "title": "Interference of anti-allotype antisera with antigen-antibody binding.", "content": "Rabbit anti-b4 antisera are capable of inhibiting the antigen-binding ability of antibodies carrying b4 allotype. The data support the hypothesis of the localization of at least one b group allotypic determinant in the V region.", "contents": "Interference of anti-allotype antisera with antigen-antibody binding. Rabbit anti-b4 antisera are capable of inhibiting the antigen-binding ability of antibodies carrying b4 allotype. The data support the hypothesis of the localization of at least one b group allotypic determinant in the V region."}
{"id": "PMID:1090679", "title": "Demonstration and assaying of IgG antibodies in tissues and on cells by labeled staphylococcal protein A.", "content": "Staphylococcal protein  A (SpA) labeled with FITC was used to detect IgG antibodies to tissue and cell surface antigens. Radioiodinated SpA was employed in a radioimmuno technique to assay IgG antibodies which had reacted with cell membrane antigens. Red blood cells coated with SpA were used as an indicator system in the mixed haemadsorption technique to detect IgG antibodies on tissue culture cells. The results showed that SpA was highly specific in the various systems tested, although it was not as sensitive in the immunofluorescence test as FITC anti-IgG. It was confirmed that SpA reacts with IgG antibodies of human, monkey, rabbit, sheep and mice origin. The reactivity with human and monkey IgG was stronger than with IgG of the other species. We consider labeled SpA as an alternative to monospecific anti-IgG for detection and assaying of IgG antibodies to tissue and cell surface antigens.", "contents": "Demonstration and assaying of IgG antibodies in tissues and on cells by labeled staphylococcal protein A. Staphylococcal protein  A (SpA) labeled with FITC was used to detect IgG antibodies to tissue and cell surface antigens. Radioiodinated SpA was employed in a radioimmuno technique to assay IgG antibodies which had reacted with cell membrane antigens. Red blood cells coated with SpA were used as an indicator system in the mixed haemadsorption technique to detect IgG antibodies on tissue culture cells. The results showed that SpA was highly specific in the various systems tested, although it was not as sensitive in the immunofluorescence test as FITC anti-IgG. It was confirmed that SpA reacts with IgG antibodies of human, monkey, rabbit, sheep and mice origin. The reactivity with human and monkey IgG was stronger than with IgG of the other species. We consider labeled SpA as an alternative to monospecific anti-IgG for detection and assaying of IgG antibodies to tissue and cell surface antigens."}
{"id": "PMID:1090685", "title": "Combined activity of amphotericin B and 5-fluorocytosine against Cryptococcus neoformans in vitro and in vivo in mice.", "content": "The in vitro and in vivo activities of amphotericin B and 5-fluorocytosine (5-FC) alone and in combination were studied to determine possible drug interactions against two strains of Cryptococcus neoformans, one sensitive to 5-FC and one resistant to 5-FC. In vitro tube dilution studies demonstrated only additive effects with the 5-FC-sensitive organism but antagonism with eth 5-FC-resistant organism. A mouse model of cryptococcal meningitis allowed comparative drug trails in a new model for the detection of drug interactions. Drug combinations were no more effective against meningitis caused by the 5-FC-sensitive organism than the additive effects of the individual drugs. However, meningitis caused by the 5-FC-resistant Cryptococcus responded less to drug combinations than to either drug alone. Serum levels of amphotericin B and 5-FC were comparable in all groups. No evidence of toxicity from the drug combinations was found. No inhibition of development of resistance to 5-FC by the combination with amphotericin B was detected.", "contents": "Combined activity of amphotericin B and 5-fluorocytosine against Cryptococcus neoformans in vitro and in vivo in mice. The in vitro and in vivo activities of amphotericin B and 5-fluorocytosine (5-FC) alone and in combination were studied to determine possible drug interactions against two strains of Cryptococcus neoformans, one sensitive to 5-FC and one resistant to 5-FC. In vitro tube dilution studies demonstrated only additive effects with the 5-FC-sensitive organism but antagonism with eth 5-FC-resistant organism. A mouse model of cryptococcal meningitis allowed comparative drug trails in a new model for the detection of drug interactions. Drug combinations were no more effective against meningitis caused by the 5-FC-sensitive organism than the additive effects of the individual drugs. However, meningitis caused by the 5-FC-resistant Cryptococcus responded less to drug combinations than to either drug alone. Serum levels of amphotericin B and 5-FC were comparable in all groups. No evidence of toxicity from the drug combinations was found. No inhibition of development of resistance to 5-FC by the combination with amphotericin B was detected."}
{"id": "PMID:1090686", "title": "Use of erythrocytes sensitized with purified enterotoxin from Vibrio cholerae for the assay of antibody and antibody-forming cells.", "content": "A method was developed for the sensitization of ovine erythrocytes with a purified enterotoxin from Vibrio cholerae. Sensitized cells were used for the titration of serum antibody by passive hemagglutination and in a hemolytic plaque assay for both IgM and IgG antibody-secreting cells. Inhibition experiments with various antigens of V. cholerae indicated that the toxin, whether unheated or heat-inactivated, significantly reduced the expected antitoxic plaque-forming cell response, whereas a lipopolysaccharide-rich extract from homologous vibiros was not inhibitory.", "contents": "Use of erythrocytes sensitized with purified enterotoxin from Vibrio cholerae for the assay of antibody and antibody-forming cells. A method was developed for the sensitization of ovine erythrocytes with a purified enterotoxin from Vibrio cholerae. Sensitized cells were used for the titration of serum antibody by passive hemagglutination and in a hemolytic plaque assay for both IgM and IgG antibody-secreting cells. Inhibition experiments with various antigens of V. cholerae indicated that the toxin, whether unheated or heat-inactivated, significantly reduced the expected antitoxic plaque-forming cell response, whereas a lipopolysaccharide-rich extract from homologous vibiros was not inhibitory."}
{"id": "PMID:1090687", "title": "Microplaque reduction: new assay for neutralizing antibody to lymphocytic choriomeningitis virus.", "content": "A plaque-reduction neutralization test for lymphocytic choriomeningitis virus was developed; microtiter dilution techniques and assay plates were used for the new test. Plaques develop by day 4 after inoculation of serum and lymphocytic chorio-meningitis virus into 5-mm cups containing agarose suspension of baby hamster kidney cells. The method lends itself well to the titration of neutralizing antibody and gives rapid results with great economy of reagents. When tested with one batch of 55 lymphocytic choriomeningitis virus-positive sera, the plaque reduction test was more senstive for detection of specific antibody than either the immunofluorescence or the complement fixation method.", "contents": "Microplaque reduction: new assay for neutralizing antibody to lymphocytic choriomeningitis virus. A plaque-reduction neutralization test for lymphocytic choriomeningitis virus was developed; microtiter dilution techniques and assay plates were used for the new test. Plaques develop by day 4 after inoculation of serum and lymphocytic chorio-meningitis virus into 5-mm cups containing agarose suspension of baby hamster kidney cells. The method lends itself well to the titration of neutralizing antibody and gives rapid results with great economy of reagents. When tested with one batch of 55 lymphocytic choriomeningitis virus-positive sera, the plaque reduction test was more senstive for detection of specific antibody than either the immunofluorescence or the complement fixation method."}
{"id": "PMID:1090688", "title": "Rheumatoid behavior in normal human synovial fibroblasts induced by extracts of Gram-negative bacteria.", "content": "We have previously shown that exposure of normal human synovial cells to whole extracts of Gram-negative bacteria or purified endotoxins results in increased synthesis of hyaluronic acid, and increased glucose uptake and lactate output. Derivatives of Gram-negative bacteria, therefore, duplicate the major known actions of connective tissue activating peptide (CTAP). The studies presented here demonstrate that, in addition to the hypermetabolic changes, extract-treated synovial cells become hyposensitive to CTAP and acquire accelerated growth rates. Furthermore, the cellular alterations which occur following a short bacterial product-synovial fibroblast interaction period (72 hours) persist for varying lengths of time, in some cases for as long as the cells are followed in culture (up to 38 days). Additional data presented suggest that bacterial products induce CTAP formation or activation within synovial fibroblasts. Pre-incubation of bacterial endotoxin in fresh normal human serum does not alter its ability to activate synovial cells. Many of the cellular alterations induced by bacterial products and endotoxin (derivatives of infectious agents) resemble the abnormal metabolism of rheumatoid synovium in vivo and are characteristic of the behavior of rheumatoid connective tissue fibroblasts grown in vitro. Furthermore, the persistence of the bacterial extract-induced behavior resembles the semi-autonomy of the cultured rheumatoid synovial cell, at least for a limited period of observation.", "contents": "Rheumatoid behavior in normal human synovial fibroblasts induced by extracts of Gram-negative bacteria. We have previously shown that exposure of normal human synovial cells to whole extracts of Gram-negative bacteria or purified endotoxins results in increased synthesis of hyaluronic acid, and increased glucose uptake and lactate output. Derivatives of Gram-negative bacteria, therefore, duplicate the major known actions of connective tissue activating peptide (CTAP). The studies presented here demonstrate that, in addition to the hypermetabolic changes, extract-treated synovial cells become hyposensitive to CTAP and acquire accelerated growth rates. Furthermore, the cellular alterations which occur following a short bacterial product-synovial fibroblast interaction period (72 hours) persist for varying lengths of time, in some cases for as long as the cells are followed in culture (up to 38 days). Additional data presented suggest that bacterial products induce CTAP formation or activation within synovial fibroblasts. Pre-incubation of bacterial endotoxin in fresh normal human serum does not alter its ability to activate synovial cells. Many of the cellular alterations induced by bacterial products and endotoxin (derivatives of infectious agents) resemble the abnormal metabolism of rheumatoid synovium in vivo and are characteristic of the behavior of rheumatoid connective tissue fibroblasts grown in vitro. Furthermore, the persistence of the bacterial extract-induced behavior resembles the semi-autonomy of the cultured rheumatoid synovial cell, at least for a limited period of observation."}
{"id": "PMID:1090689", "title": "Effects of castration, and testosterone in vitro, on the hypothalamic synthesis of different peptide fractions.", "content": "The incorporation of [3H]tyrosine ([3H]tyr) into different hypothalamic peptide fractions isolated from normal and castrated rats on a Sephadex G-25 column has been studied in vitro. Luteinizing hormone releasing factor (LH-RF) activity was determined in the different fractions by measuring their ability to elicit release of radioimmunoassayable luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in ovariectomized rats treated with oestrogen and progesterone. For a further purification, the fraction with LH-RF activity was applied to a CM-Sephadex G-25 column eluted with a gradient of ammonium acetate. The large radioactive peptides emerged from the Sephadex G-25 column in fraction S-1, while the small peptides with LH-RF activity were eluted in fraction S-2. Gonadectomy significantly increased the incorporation of [3H]tyr into the peptides of fractions S-2. Only in the purified fraction with LH-RF activity was the radioactivity incorporated higher in gonadectomized than in normal rats. The enhanced incorporation in fraction CM-3 observed after castration implies an increase in the hypothalamic synthesis of peptides with LH-RF activity. The addition of testosterone (2 mug/ml) to the incubation medium of hypothalamus from gonadectomized rats, corrected these modifications. Gonadectomy decreased the incorporation of tyrosine into the large peptides, and incubation with testosterone corrected this change. The modifications in the incorporation of [3H]tyr into the large and small peptides produced by castration appear to indicate that gonadectomy, as well as stimulating the production of LH-RF, enhances the synthesis of other hypothalamic peptides while inhibiting the synthesis of proteins. On the other hand an increase in the breakdown of large peptides into small peptides cannot be excluded.", "contents": "Effects of castration, and testosterone in vitro, on the hypothalamic synthesis of different peptide fractions. The incorporation of [3H]tyrosine ([3H]tyr) into different hypothalamic peptide fractions isolated from normal and castrated rats on a Sephadex G-25 column has been studied in vitro. Luteinizing hormone releasing factor (LH-RF) activity was determined in the different fractions by measuring their ability to elicit release of radioimmunoassayable luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in ovariectomized rats treated with oestrogen and progesterone. For a further purification, the fraction with LH-RF activity was applied to a CM-Sephadex G-25 column eluted with a gradient of ammonium acetate. The large radioactive peptides emerged from the Sephadex G-25 column in fraction S-1, while the small peptides with LH-RF activity were eluted in fraction S-2. Gonadectomy significantly increased the incorporation of [3H]tyr into the peptides of fractions S-2. Only in the purified fraction with LH-RF activity was the radioactivity incorporated higher in gonadectomized than in normal rats. The enhanced incorporation in fraction CM-3 observed after castration implies an increase in the hypothalamic synthesis of peptides with LH-RF activity. The addition of testosterone (2 mug/ml) to the incubation medium of hypothalamus from gonadectomized rats, corrected these modifications. Gonadectomy decreased the incorporation of tyrosine into the large peptides, and incubation with testosterone corrected this change. The modifications in the incorporation of [3H]tyr into the large and small peptides produced by castration appear to indicate that gonadectomy, as well as stimulating the production of LH-RF, enhances the synthesis of other hypothalamic peptides while inhibiting the synthesis of proteins. On the other hand an increase in the breakdown of large peptides into small peptides cannot be excluded."}
{"id": "PMID:1090690", "title": "The temporal relationship between the secretion of luteinizing hormone and testosterone in man.", "content": "It has been suggested recently that testosterone secretion by the human testis may be controlled by factors other than luteinizing hormone (LH). In order to re-examine this hypothesis, plasma LH and testosterone concentrations were determined throughout the day in eight studies. A new method of data analysis revealed that the levels of the two hormones were closely related, but that the testicular response to LH was sluggish. These results explain some inconsistencies in the literature. It was demonstrated that average values for LH varied throughout the day, with a morning maximum and an evening minimum. It was also shown that injections of LH releasing hormone in man resulted in an increase in plasma testosterone above control levels. These results are consistent with the concept that LH controls the major changes in testosterone secretion in men. They do not exclude, however, the possible existence of other factors which might affect the peripheral concentration of testosterone, such as changes in testicular blood flow.", "contents": "The temporal relationship between the secretion of luteinizing hormone and testosterone in man. It has been suggested recently that testosterone secretion by the human testis may be controlled by factors other than luteinizing hormone (LH). In order to re-examine this hypothesis, plasma LH and testosterone concentrations were determined throughout the day in eight studies. A new method of data analysis revealed that the levels of the two hormones were closely related, but that the testicular response to LH was sluggish. These results explain some inconsistencies in the literature. It was demonstrated that average values for LH varied throughout the day, with a morning maximum and an evening minimum. It was also shown that injections of LH releasing hormone in man resulted in an increase in plasma testosterone above control levels. These results are consistent with the concept that LH controls the major changes in testosterone secretion in men. They do not exclude, however, the possible existence of other factors which might affect the peripheral concentration of testosterone, such as changes in testicular blood flow."}
{"id": "PMID:1090692", "title": "Luteinizing hormone release in entire and castrated rams following injection of synthetic luteinizing hormone releasing hormone, and effect of testosterone propionate pre-treatment.", "content": "Plasma luteinizing hormone (LH) levels and LH responses to intravenous administration of 100 mug luteinizing hormone releasing hormone (LH-RH) were studied in entire rams, long-term castrated animals (operation performed six months previously), long-term castrated animals treated with testosterone for the two prededing weeks and short-term castrated animals (castrated 3 h before LH-RH injection). LH was measured by radioimmunoassay in samples taken at 5 or 15 min intervals. Basal LH levels were lower in entire rams (0-9 ng/ml) than in long-term castrated animals (6-0 ng/nl). After LH-RH treatment the LH response was much smaller (peak level 9-6 ng/ml), total response 13-3 ng/ml/1 h) and slower (120 min to peak) in entire than in long-term castrated animals (peak level 61-8 ng/ml, total response 141-2 ng/ml/1 h, 29 min to peak). Testosterone treatment after long-term castration depressed the basal LH level and delayed the peak LH response after LH-RH to values similar to those for entire rams. After short-term castration the response to LH-RH was already as great (peak level 70-1 ng/ml, total response 133-6 ng/ml/1 h) as after long-term castration. The latency to peak LH level (82 min) was intermediate between that for untreated and testosterone-treated long-term castrated animals (130 min). Testosterone treatment was considered to have acted on the hypothalamus to depress basal levels. The results provided evidence for the presence of two inhibitory actions of the testis at the pituitary level in the ram: a qualitative delaying action of testosterone and a quantitative inhibitory action of the testis on LH release after LH-RH injection. The latter may also be related to plasma testosterone levels.", "contents": "Luteinizing hormone release in entire and castrated rams following injection of synthetic luteinizing hormone releasing hormone, and effect of testosterone propionate pre-treatment. Plasma luteinizing hormone (LH) levels and LH responses to intravenous administration of 100 mug luteinizing hormone releasing hormone (LH-RH) were studied in entire rams, long-term castrated animals (operation performed six months previously), long-term castrated animals treated with testosterone for the two prededing weeks and short-term castrated animals (castrated 3 h before LH-RH injection). LH was measured by radioimmunoassay in samples taken at 5 or 15 min intervals. Basal LH levels were lower in entire rams (0-9 ng/ml) than in long-term castrated animals (6-0 ng/nl). After LH-RH treatment the LH response was much smaller (peak level 9-6 ng/ml), total response 13-3 ng/ml/1 h) and slower (120 min to peak) in entire than in long-term castrated animals (peak level 61-8 ng/ml, total response 141-2 ng/ml/1 h, 29 min to peak). Testosterone treatment after long-term castration depressed the basal LH level and delayed the peak LH response after LH-RH to values similar to those for entire rams. After short-term castration the response to LH-RH was already as great (peak level 70-1 ng/ml, total response 133-6 ng/ml/1 h) as after long-term castration. The latency to peak LH level (82 min) was intermediate between that for untreated and testosterone-treated long-term castrated animals (130 min). Testosterone treatment was considered to have acted on the hypothalamus to depress basal levels. The results provided evidence for the presence of two inhibitory actions of the testis at the pituitary level in the ram: a qualitative delaying action of testosterone and a quantitative inhibitory action of the testis on LH release after LH-RH injection. The latter may also be related to plasma testosterone levels."}
{"id": "PMID:1090693", "title": "The secretion and metabolic clearance rates of oestradiol in the rat.", "content": "The secretion rate (SR) of oestradiol in rats at different stages of the oestrous cycle was determined by measuring the concentration of this hormone in ovarian vein plasma by a competitive protein binding method utilizing rabbit uterine cytosol. The plasma metabolic clearance rate (MCRP) for oestradiol was determined in groups of rats of the same strain and housed under the same conditions by giving a single intravenous injection of tritiated oestradiol-17beta and measuring the rate of disappearance of labelled hormone from the plasma. From the relationship SR equals MCRP times plasma concentration, predicted values for the plasma oestradiol concentration were obtained and shown to be close to those obtained by direct analysis of peripheral plasma. The MCRP for oestradiol was not greatly influenced by the stage of the oestrous cycle, the dose of oestradiol-17beta injected, ovariectomy 18 h previously or by a sham-operation immediately before the injection of labelled hormone. The anaesthetic used did affect the MCRP ; the value for adult female rats injected under ether anaesthesia was about 970 ml/h but under Avertin anaesthesia clearance rates of about 1500 ml/h were obtained. Ligation of the bile duct acutely reduced the MCRP but functional nephrectomy had no immediate effect. Sub-total hepatectomy 18 h previously reduced the MCRP considerably. A small decrease in MCRP was observed when the labelled hormone was administered in third trimester human pregnancy plasma and the MCRP was lower in rats on Day 14 of pregnancy than in rats examined during the oestrous cycle.", "contents": "The secretion and metabolic clearance rates of oestradiol in the rat. The secretion rate (SR) of oestradiol in rats at different stages of the oestrous cycle was determined by measuring the concentration of this hormone in ovarian vein plasma by a competitive protein binding method utilizing rabbit uterine cytosol. The plasma metabolic clearance rate (MCRP) for oestradiol was determined in groups of rats of the same strain and housed under the same conditions by giving a single intravenous injection of tritiated oestradiol-17beta and measuring the rate of disappearance of labelled hormone from the plasma. From the relationship SR equals MCRP times plasma concentration, predicted values for the plasma oestradiol concentration were obtained and shown to be close to those obtained by direct analysis of peripheral plasma. The MCRP for oestradiol was not greatly influenced by the stage of the oestrous cycle, the dose of oestradiol-17beta injected, ovariectomy 18 h previously or by a sham-operation immediately before the injection of labelled hormone. The anaesthetic used did affect the MCRP ; the value for adult female rats injected under ether anaesthesia was about 970 ml/h but under Avertin anaesthesia clearance rates of about 1500 ml/h were obtained. Ligation of the bile duct acutely reduced the MCRP but functional nephrectomy had no immediate effect. Sub-total hepatectomy 18 h previously reduced the MCRP considerably. A small decrease in MCRP was observed when the labelled hormone was administered in third trimester human pregnancy plasma and the MCRP was lower in rats on Day 14 of pregnancy than in rats examined during the oestrous cycle."}
{"id": "PMID:1090694", "title": "Development of pathways of insulin secretion in the rabbit.", "content": "Insulin release was studied in vitro using pieces of pancreas from rabbits of between 24 days gestational age and 6 weeks postnatal age. When allowance was made for the fraction of pancreas which was endocrine, 16-5mM-glucose caused increasing stimulation of insulin release as development advanced and 3-3 mM-glucose caused a similar rate of secretion at all ages. Secretion was not significantly influenced by insulin destruction in the incubation medium. Glucagon (5 mug/ml) did not stimulate insulin secretion from 24-day foetal pancreas but did so postnatally. Theophylline (1 mmol/1) stimulated insulin release at all ages and was equipotent on 24-day foetal pancreas in 3-3 or 16-5 mM-glucose. The stimulation of insulin release from 24-day foetal pancreas by 1 mM-theophylline occurred in the absence of extracellular glucose, pyruvate, fumarate and glutamate and in the presence of mannoheptulose and 2-deoxyglucose (each 3 mg/ml). Adrenaline (1 mumol/1) and diazoxide (250 mug/ml) abolished or attenuated the stimulation of insulin release by glucose, leucine plus arginine or theophylline from 24-day foetal, 1 day and 6 weeks postnatal pancreas. The stimulation of insulin release from 6-week-old pancreas by 1mM-barium was blocked by adrenaline and diazoxide but the effect became less with increasing immaturity. The experimental results illustrate some of the ways in which insulin secretion by the rabbit beta cell changes as a function of development and draw attention to the importance of glucose and cyclic adenosine monophosphate in this process.", "contents": "Development of pathways of insulin secretion in the rabbit. Insulin release was studied in vitro using pieces of pancreas from rabbits of between 24 days gestational age and 6 weeks postnatal age. When allowance was made for the fraction of pancreas which was endocrine, 16-5mM-glucose caused increasing stimulation of insulin release as development advanced and 3-3 mM-glucose caused a similar rate of secretion at all ages. Secretion was not significantly influenced by insulin destruction in the incubation medium. Glucagon (5 mug/ml) did not stimulate insulin secretion from 24-day foetal pancreas but did so postnatally. Theophylline (1 mmol/1) stimulated insulin release at all ages and was equipotent on 24-day foetal pancreas in 3-3 or 16-5 mM-glucose. The stimulation of insulin release from 24-day foetal pancreas by 1 mM-theophylline occurred in the absence of extracellular glucose, pyruvate, fumarate and glutamate and in the presence of mannoheptulose and 2-deoxyglucose (each 3 mg/ml). Adrenaline (1 mumol/1) and diazoxide (250 mug/ml) abolished or attenuated the stimulation of insulin release by glucose, leucine plus arginine or theophylline from 24-day foetal, 1 day and 6 weeks postnatal pancreas. The stimulation of insulin release from 6-week-old pancreas by 1mM-barium was blocked by adrenaline and diazoxide but the effect became less with increasing immaturity. The experimental results illustrate some of the ways in which insulin secretion by the rabbit beta cell changes as a function of development and draw attention to the importance of glucose and cyclic adenosine monophosphate in this process."}
{"id": "PMID:1090696", "title": "Mass isolation and culture of rat kupffer cells.", "content": "Collagenase perfusion of the liver followed by pronase treatment of the cell suspension thus obtained gave a quantitative recovery of viable nonparenchymal liver cells (NPC). From these NPC, Kupffer (K) cells can be purified by attachment to tissue culture dishes. Tail vein injection of carbon 1-2 h before liver perfusion permitted stepwise calculation as well as visualization of carbon-containing K cells. When these K cells have been put into tissue culture medium with serum and incubated overnight, they exhibit typical macrophage characteristics. Phase-contrast and transmission electron microscopy showed typical macrophage morphology and scanning electron microscopy revealed well-spread cells with cytoplasmic projections and ruffled membranes. Endocytosis studies using radioactive colloidal gold and inert latex particles also indicated that these cells are highly active in pinocytosis and phagocytosis. Further characterization of K cells is the identification of Fc receptor on their membranes. Studies on lysosomal enzymes showed that purified K cells possess higher specific activities in beta-glucuronidase, acid DNase, and cathepsin D than in purified parenchymal cells.", "contents": "Mass isolation and culture of rat kupffer cells. Collagenase perfusion of the liver followed by pronase treatment of the cell suspension thus obtained gave a quantitative recovery of viable nonparenchymal liver cells (NPC). From these NPC, Kupffer (K) cells can be purified by attachment to tissue culture dishes. Tail vein injection of carbon 1-2 h before liver perfusion permitted stepwise calculation as well as visualization of carbon-containing K cells. When these K cells have been put into tissue culture medium with serum and incubated overnight, they exhibit typical macrophage characteristics. Phase-contrast and transmission electron microscopy showed typical macrophage morphology and scanning electron microscopy revealed well-spread cells with cytoplasmic projections and ruffled membranes. Endocytosis studies using radioactive colloidal gold and inert latex particles also indicated that these cells are highly active in pinocytosis and phagocytosis. Further characterization of K cells is the identification of Fc receptor on their membranes. Studies on lysosomal enzymes showed that purified K cells possess higher specific activities in beta-glucuronidase, acid DNase, and cathepsin D than in purified parenchymal cells."}
{"id": "PMID:1090697", "title": "Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny.", "content": "An Ig molecule containing L chains and H chains similar to human delta-chains has been detected on the surface of radioiodinated murine lymphoid cells. Newborn mice have only IgM on their splenocytes. Between 10 and 15 days, the IgD-like molecule appears and increases in amount until 3 mo of age, when it is the predominant cell surface Ig in terms of radioactivity. IgD is found only in peripheral lymphoid tissues and is present in larger amounts on peripheral lymph node cells (approximately 85% of surface Ig) than on splenocytes (approximately 50%). IgD is also present in comparable amounts on cells from both nu/nu and germfree mice, indicating that its expression may be independent of both thymic influence and antigenic stimulation. These studies suggest that there is a switch from cell surface IgM to IgD that occurs during differentiation of virgin B lymphocytes in the spleen.", "contents": "Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny. An Ig molecule containing L chains and H chains similar to human delta-chains has been detected on the surface of radioiodinated murine lymphoid cells. Newborn mice have only IgM on their splenocytes. Between 10 and 15 days, the IgD-like molecule appears and increases in amount until 3 mo of age, when it is the predominant cell surface Ig in terms of radioactivity. IgD is found only in peripheral lymphoid tissues and is present in larger amounts on peripheral lymph node cells (approximately 85% of surface Ig) than on splenocytes (approximately 50%). IgD is also present in comparable amounts on cells from both nu/nu and germfree mice, indicating that its expression may be independent of both thymic influence and antigenic stimulation. These studies suggest that there is a switch from cell surface IgM to IgD that occurs during differentiation of virgin B lymphocytes in the spleen."}
{"id": "PMID:1090698", "title": "Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex.", "content": "The results of this study provide compelling evidence for the existence of the gene or genes controlling optimal T-B-cell cooperative interactions in the designated I region of the H-2 gene complex. Previously, we have speculated that the relevant gene(s) involved may well be located in this region based on several observations from our earlier work in this area (3, 5, 6). Thus, in the preceding paper, we showed that T and B cells from B10.BR and A strain mice developed effective cooperative interactions in vitro to DNP-KLH in a system identical to the one reported herein. Since these mice differ for genes in the S and D regions of H-2 but are identical for K and I region genes, we were able to localize the critical genes to the K-end of H-2.", "contents": "Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex. The results of this study provide compelling evidence for the existence of the gene or genes controlling optimal T-B-cell cooperative interactions in the designated I region of the H-2 gene complex. Previously, we have speculated that the relevant gene(s) involved may well be located in this region based on several observations from our earlier work in this area (3, 5, 6). Thus, in the preceding paper, we showed that T and B cells from B10.BR and A strain mice developed effective cooperative interactions in vitro to DNP-KLH in a system identical to the one reported herein. Since these mice differ for genes in the S and D regions of H-2 but are identical for K and I region genes, we were able to localize the critical genes to the K-end of H-2."}
{"id": "PMID:1090699", "title": "Guinea pig immune response-related histocompatibility antigens. Partial characterization and distribution.", "content": "We have previously demonstrated that guinea pig alloantisera directed at strain 2 and strain 13 membrane antigens block specific lymphocyte activation in immune response gene-controlled systems. In this communication we describe the partial characterization of the antigens against which these antisera are directed (the 2 and 13 antigens) and, in addition, that of the B antigen which by distribution resembles the human HL-A and mouse H-2 major histocompatibility antigens. Lymphoid cells from strain 2 and strain 13 guinea pigs were surface labeled with 125I by the lactoperoxidase technique. Nonidet P-40 extracts of these labeled cells were precipitated by sandwiches of strain 2 antistrain 13, strain 13 antistrain 2, or outbred anti-B antisera, followed by rabbit antiguinea pig immunoglobulin antisera. Precipitates were dissolved in sodium dodecyl sulfate (SDS) and electrophoresed on SDS polyacrylamide gels. Radioactive peaks representing the 2 and B-cell membrane antigens were obtained from strain 2 lymph node cells, as well as from a B-lymphoid cell population (L2C leukemia cells) and a T-lymphocyte population (STRAIN 2 PERITONEAL EXUDATE LYMPHOCYTES [PELs]). Radioactive peaks representing the 13 and B-cell membrane antigens were obtained from strain 13 lymph node cells and strain 13 PELs. All anti-B precipitates produced two peaks when electrophoresed on SDS polyacrylamide gels; one representing an antigen with a mol wt of approximately 45,000, and one representing an antigen with a mol wt of about 12,000. Both may be components of a single protein. All anti-2 and anti-13 precipitates produced a single peak when electrophoresed on SDS polyacrylamide gels. Both the 2 and 13 antigens were found by this technique to have mol wt of approximately 25,000. By molecular weight criteria, as well as by previously investigated distributional criteria, the B antigen is similar to the human LA and Four antigens, and to the mouse D and K antigens, and the 2 and 13 antigens are similar to the mouse Ia antigens.", "contents": "Guinea pig immune response-related histocompatibility antigens. Partial characterization and distribution. We have previously demonstrated that guinea pig alloantisera directed at strain 2 and strain 13 membrane antigens block specific lymphocyte activation in immune response gene-controlled systems. In this communication we describe the partial characterization of the antigens against which these antisera are directed (the 2 and 13 antigens) and, in addition, that of the B antigen which by distribution resembles the human HL-A and mouse H-2 major histocompatibility antigens. Lymphoid cells from strain 2 and strain 13 guinea pigs were surface labeled with 125I by the lactoperoxidase technique. Nonidet P-40 extracts of these labeled cells were precipitated by sandwiches of strain 2 antistrain 13, strain 13 antistrain 2, or outbred anti-B antisera, followed by rabbit antiguinea pig immunoglobulin antisera. Precipitates were dissolved in sodium dodecyl sulfate (SDS) and electrophoresed on SDS polyacrylamide gels. Radioactive peaks representing the 2 and B-cell membrane antigens were obtained from strain 2 lymph node cells, as well as from a B-lymphoid cell population (L2C leukemia cells) and a T-lymphocyte population (STRAIN 2 PERITONEAL EXUDATE LYMPHOCYTES [PELs]). Radioactive peaks representing the 13 and B-cell membrane antigens were obtained from strain 13 lymph node cells and strain 13 PELs. All anti-B precipitates produced two peaks when electrophoresed on SDS polyacrylamide gels; one representing an antigen with a mol wt of approximately 45,000, and one representing an antigen with a mol wt of about 12,000. Both may be components of a single protein. All anti-2 and anti-13 precipitates produced a single peak when electrophoresed on SDS polyacrylamide gels. Both the 2 and 13 antigens were found by this technique to have mol wt of approximately 25,000. By molecular weight criteria, as well as by previously investigated distributional criteria, the B antigen is similar to the human LA and Four antigens, and to the mouse D and K antigens, and the 2 and 13 antigens are similar to the mouse Ia antigens."}
{"id": "PMID:1090700", "title": "A stable form of delayed-type hypersensitivity.", "content": "An antigen dose below the level needed to provoke an antibody response produces in mice a persistent, but minor degree of delayed-type hypersensitivity (dth) to sheep red blood cells. The DTH is unstable. It is erased by larger doses of antigen and cannot be built upon by further antigenic stimulation. The much higher levels of DTH resulting from immunization under the modulating influence of cyclophosphamide (CY) or BCG persist under strong secondary antigenic stimulation, though the former is subject to partial suppression unless CY is used to prevent the secondary humoral response. The DTH produced by a BCG-modulated primary response is not subject to this suppressive effect of a secondary antibody response. In this case the anamnestic T-cell response is very brisk and cannont be potentiated by giving CY at the time of the secondary antigenic stimulus. This effect is not due to the modulating influence of a residual BCG infection. It results from a permanent change induced during the primary response. The mediator cells formed under the influence of BCG are apparently resistant to inhibition by blocking serum containing immune complexes. Even the actively dividing T cells which are susceptible to vinblastine, and most readily blocked in the absence of BCG, are highly resistant to blocking by immune complexes. It is not clear whether these cells are intrinsically different or whether their insensitivity to blocking results from features peculiar to the humoral response that accompanies a BCG-modulated primary response. The mediator cells produced by both BCG- and CY-modulated responses become vinblastine resistant, relatively insensitive to humoral blocking factors, and capable of surviving in a functionally active form in syngeneic recipients with an apparent half-life of about 50 days. There were indications, however, that their effective life-span may be greatly extended in some circumstances by persisting antigenic stimulation; and in the case of BCG-modulated immunity the prevailing level of T-cell activity can be greatly augmented by a further antigenic stimulus without the necessity for renewed exposure to BCG.", "contents": "A stable form of delayed-type hypersensitivity. An antigen dose below the level needed to provoke an antibody response produces in mice a persistent, but minor degree of delayed-type hypersensitivity (dth) to sheep red blood cells. The DTH is unstable. It is erased by larger doses of antigen and cannot be built upon by further antigenic stimulation. The much higher levels of DTH resulting from immunization under the modulating influence of cyclophosphamide (CY) or BCG persist under strong secondary antigenic stimulation, though the former is subject to partial suppression unless CY is used to prevent the secondary humoral response. The DTH produced by a BCG-modulated primary response is not subject to this suppressive effect of a secondary antibody response. In this case the anamnestic T-cell response is very brisk and cannont be potentiated by giving CY at the time of the secondary antigenic stimulus. This effect is not due to the modulating influence of a residual BCG infection. It results from a permanent change induced during the primary response. The mediator cells formed under the influence of BCG are apparently resistant to inhibition by blocking serum containing immune complexes. Even the actively dividing T cells which are susceptible to vinblastine, and most readily blocked in the absence of BCG, are highly resistant to blocking by immune complexes. It is not clear whether these cells are intrinsically different or whether their insensitivity to blocking results from features peculiar to the humoral response that accompanies a BCG-modulated primary response. The mediator cells produced by both BCG- and CY-modulated responses become vinblastine resistant, relatively insensitive to humoral blocking factors, and capable of surviving in a functionally active form in syngeneic recipients with an apparent half-life of about 50 days. There were indications, however, that their effective life-span may be greatly extended in some circumstances by persisting antigenic stimulation; and in the case of BCG-modulated immunity the prevailing level of T-cell activity can be greatly augmented by a further antigenic stimulus without the necessity for renewed exposure to BCG."}
{"id": "PMID:1090701", "title": "Rosette-forming ability of thymus-derived lymphocytes in humoral and cell-mediated immunity. II. Helper cell activity.", "content": "The technique of velocity sedimentation at unit gravity was used to determine the size and rosette-forming ability of helper cells in nonimmune and immune C57BL/6 mice. Helper T cells were assayed by the ability to cooperate with bone barrow (B) cells in the antibody response to sheep erythrocytes (SRBC) in vivo. 19S helper cells in nonimmune animals were nonrosette-forming small lymphocytes; after immunization with SRBC in complete Freund's adjuvant, 19S helper cells were nonrosette-forming medium lymphocytes. 7S helper cells in immune animals were small lymphocytes which did not form rosettes. No SRBC binding by helper cells was observed under the conditions of rosette formation used.", "contents": "Rosette-forming ability of thymus-derived lymphocytes in humoral and cell-mediated immunity. II. Helper cell activity. The technique of velocity sedimentation at unit gravity was used to determine the size and rosette-forming ability of helper cells in nonimmune and immune C57BL/6 mice. Helper T cells were assayed by the ability to cooperate with bone barrow (B) cells in the antibody response to sheep erythrocytes (SRBC) in vivo. 19S helper cells in nonimmune animals were nonrosette-forming small lymphocytes; after immunization with SRBC in complete Freund's adjuvant, 19S helper cells were nonrosette-forming medium lymphocytes. 7S helper cells in immune animals were small lymphocytes which did not form rosettes. No SRBC binding by helper cells was observed under the conditions of rosette formation used."}
{"id": "PMID:1090702", "title": "Role of C'3 and Fc receptors in B-lymphocyte activation.", "content": "Attempts were made to identify the non-Ig lymphocyte receptor responsible for B-cell induction by antigen and polyclonal B-cell activators (PBA). As a first step, the role of C'3 and Fc receptors was analyzed. It was shown that complement could be fixed onto B cells to such an extent that the lymphocytes could not bind complement-coated red cells, but this did not result in induction of polyclonal antibody synthesis, nor did it inhibit the lymphocytes response to PBA. However, the C'3 receptros possessed a passive focussing role in the induction of polyclonal antibody responses. Thus, PBA that had fixed complement activated polyclonal responses at lower concentrations than the same substances that had not fixed complement. Most likely the dual binding of PBA molecules to B cells by the PBA and the C'3 receptors caused more PBA molecules to be bound to each cell. However, the focussing function of the C'3 receptors was several orders of magnitude smaller than that of the Ig receptors. Analogous studies were carried out with Fc receptors. Binding of different types of antigen-antibody complexes did not cause activation of polyclonal or specific antibody synthesis, nor did it significantly interfere with induction of antibody synthesis by PBA substances.", "contents": "Role of C'3 and Fc receptors in B-lymphocyte activation. Attempts were made to identify the non-Ig lymphocyte receptor responsible for B-cell induction by antigen and polyclonal B-cell activators (PBA). As a first step, the role of C'3 and Fc receptors was analyzed. It was shown that complement could be fixed onto B cells to such an extent that the lymphocytes could not bind complement-coated red cells, but this did not result in induction of polyclonal antibody synthesis, nor did it inhibit the lymphocytes response to PBA. However, the C'3 receptros possessed a passive focussing role in the induction of polyclonal antibody responses. Thus, PBA that had fixed complement activated polyclonal responses at lower concentrations than the same substances that had not fixed complement. Most likely the dual binding of PBA molecules to B cells by the PBA and the C'3 receptors caused more PBA molecules to be bound to each cell. However, the focussing function of the C'3 receptors was several orders of magnitude smaller than that of the Ig receptors. Analogous studies were carried out with Fc receptors. Binding of different types of antigen-antibody complexes did not cause activation of polyclonal or specific antibody synthesis, nor did it significantly interfere with induction of antibody synthesis by PBA substances."}
{"id": "PMID:1090705", "title": "The Clifford Irving hoax of the Howard Hughes autobiography.", "content": "The problem was to determine whether Howard R. Hughes, Edith Irving, or Clifford Irving wrote the questioned material Exhibits Q-1 through Q-35. The findings were as follows. 1. Howard R. Hughes was eliminated as the possible writer of the questioned handwriting on Exhibits Q-1 through Q-35. Although there were pictorial resemblances between Hughes' handwriting and the questioned material enumerated above, significant characteristic differences were also present which unequivocally eliminated him as the writer. 2. Edith Irving was identified as the writer of the \"Edith Sommer for deposit only\" endorsement on Exhibit Q-11. 3. Edith Irving also very probably wrote the \"Hanne Rosenkrantz\" on Exhibit Q-27 through Q-34, but the questioned writings were restricted to the examination of poor quality photocopies which did not provide proper basis for the handwriting comparison. 4. There were indications that Edith Irving also may have written the questioned endorsement \"H. R. Hughes\" on Exhibit Q-3 and the questioned signatures \"H. R. Hughes\" on Exhibits Q-4, Q-5, Q-6, Q-7, and Q-35, by simulating the handwriting of Howard R. Hughes on those questioned exhibits. She could not be identified, however, based on the available handwriting specimens obtained from the federal grand jury on 8 Feb. 1972. 5. Clifford Irving was identified as the writer of the questioned material on Exhibits Q-1, Q-2, Q-8, Q-9, Q-12a and Q-13a and Q-13b, Q-14, Q-15a through Q-15c, Q-16a and Q-16b, Q-22a through Q-22i, Q-23, Q-24, Q-25, and Q-26. A study of the questioned handwriting with the known handwriting of Howard R. Hughes revealed a definite attempt on the part of Irving to simulate the handwriting of Hughes, having seen Hughes' writing in earlier issues of Time-Life.", "contents": "The Clifford Irving hoax of the Howard Hughes autobiography. The problem was to determine whether Howard R. Hughes, Edith Irving, or Clifford Irving wrote the questioned material Exhibits Q-1 through Q-35. The findings were as follows. 1. Howard R. Hughes was eliminated as the possible writer of the questioned handwriting on Exhibits Q-1 through Q-35. Although there were pictorial resemblances between Hughes' handwriting and the questioned material enumerated above, significant characteristic differences were also present which unequivocally eliminated him as the writer. 2. Edith Irving was identified as the writer of the \"Edith Sommer for deposit only\" endorsement on Exhibit Q-11. 3. Edith Irving also very probably wrote the \"Hanne Rosenkrantz\" on Exhibit Q-27 through Q-34, but the questioned writings were restricted to the examination of poor quality photocopies which did not provide proper basis for the handwriting comparison. 4. There were indications that Edith Irving also may have written the questioned endorsement \"H. R. Hughes\" on Exhibit Q-3 and the questioned signatures \"H. R. Hughes\" on Exhibits Q-4, Q-5, Q-6, Q-7, and Q-35, by simulating the handwriting of Howard R. Hughes on those questioned exhibits. She could not be identified, however, based on the available handwriting specimens obtained from the federal grand jury on 8 Feb. 1972. 5. Clifford Irving was identified as the writer of the questioned material on Exhibits Q-1, Q-2, Q-8, Q-9, Q-12a and Q-13a and Q-13b, Q-14, Q-15a through Q-15c, Q-16a and Q-16b, Q-22a through Q-22i, Q-23, Q-24, Q-25, and Q-26. A study of the questioned handwriting with the known handwriting of Howard R. Hughes revealed a definite attempt on the part of Irving to simulate the handwriting of Hughes, having seen Hughes' writing in earlier issues of Time-Life."}
{"id": "PMID:1090706", "title": "Lithium in the treatment of aggression.", "content": "Lithium has become a widely accepted treatment for manic-depressive psychosis. It is dramatically effective for many cases of mania and is useful in the prevention of manic and depressive episodes. Hyperaggressiveness and hypersexuality are frequent components of manic-depressive illness and abate under the influence of lithium. A brief review is presented of the behavioral and biochemical pharmacology of lithium. This documents the inhibitory role which lithium can play in several examples of animal aggressive behavior including pain-elicited aggression, mouse killing in rats, isolation-induced aggression in mice, p-chlorophenylalanine-induced aggression in rats, and hypothalamically induced aggression in cats. The use of lithium to control human aggressive behavior has resulted in controversial findings. In epileptic conditions, improvement has been reported in interseizure aggressivity, but other reports indicate the possibility of increased seizures. Improvement in aggressive behavior in childhood has occasionally been reported as well as in emotionally unstable character disorders in young female patients. Te was a single blind study and the other a large but uncontrolled study. Both studies reported an improvement in aggressiveness as indicated by fewer recorded reports (tickets) for fighting. The final study reported is a study of 12 male delinquents age 16 to 23. They received lithium or placebo for 4 months inside an institution and then a trial of lithium for 1 to 12 months on an outpatient basis. Analysis of results in terms of the number of aggressive antisocial acts showed fewer serious aggressive episodes when the lithium level was between 0.6 and 1 meq/liter than when it was between 0.0 and 0.6 meq/liter. These results must be viewed with caution and are only suggestive since the study was not double blind.", "contents": "Lithium in the treatment of aggression. Lithium has become a widely accepted treatment for manic-depressive psychosis. It is dramatically effective for many cases of mania and is useful in the prevention of manic and depressive episodes. Hyperaggressiveness and hypersexuality are frequent components of manic-depressive illness and abate under the influence of lithium. A brief review is presented of the behavioral and biochemical pharmacology of lithium. This documents the inhibitory role which lithium can play in several examples of animal aggressive behavior including pain-elicited aggression, mouse killing in rats, isolation-induced aggression in mice, p-chlorophenylalanine-induced aggression in rats, and hypothalamically induced aggression in cats. The use of lithium to control human aggressive behavior has resulted in controversial findings. In epileptic conditions, improvement has been reported in interseizure aggressivity, but other reports indicate the possibility of increased seizures. Improvement in aggressive behavior in childhood has occasionally been reported as well as in emotionally unstable character disorders in young female patients. Te was a single blind study and the other a large but uncontrolled study. Both studies reported an improvement in aggressiveness as indicated by fewer recorded reports (tickets) for fighting. The final study reported is a study of 12 male delinquents age 16 to 23. They received lithium or placebo for 4 months inside an institution and then a trial of lithium for 1 to 12 months on an outpatient basis. Analysis of results in terms of the number of aggressive antisocial acts showed fewer serious aggressive episodes when the lithium level was between 0.6 and 1 meq/liter than when it was between 0.0 and 0.6 meq/liter. These results must be viewed with caution and are only suggestive since the study was not double blind."}
{"id": "PMID:1090708", "title": "Computerized EEG: predictor of outcome in schizophrenia.", "content": "Based on a double blind cross-over study, it was determined that schizophrenic patient who have more high frequency fast activity and a lesser degree of alpha and slow waves in computerized EEG before the treatment have a better therapeutic outcome to the major tranquilizer (neuroleptic) treatment. The correlation between pretreatment high frequency computer EEG measurements and better therapeutic outcome reached the level of statistical significance. \"Therapy resistant\" schizophrenic patients were characterized by a lesser degree of very fast beta activity, more alpha waves and slow waves, higher amplitudes in computer EEG, and a lesser degree of acute (florid) psychotic symptomatology but more \"negative\" symptoms such as motor retardation and blunted affect. One of the most striking results of the study is the finding that schizophrenic patients with certain psychopathological profiles also have similar computer EEG profiles.", "contents": "Computerized EEG: predictor of outcome in schizophrenia. Based on a double blind cross-over study, it was determined that schizophrenic patient who have more high frequency fast activity and a lesser degree of alpha and slow waves in computerized EEG before the treatment have a better therapeutic outcome to the major tranquilizer (neuroleptic) treatment. The correlation between pretreatment high frequency computer EEG measurements and better therapeutic outcome reached the level of statistical significance. \"Therapy resistant\" schizophrenic patients were characterized by a lesser degree of very fast beta activity, more alpha waves and slow waves, higher amplitudes in computer EEG, and a lesser degree of acute (florid) psychotic symptomatology but more \"negative\" symptoms such as motor retardation and blunted affect. One of the most striking results of the study is the finding that schizophrenic patients with certain psychopathological profiles also have similar computer EEG profiles."}
{"id": "PMID:1090709", "title": "Brain function and behavior. I. Emotion and sensory phenomena in psychotic patients and in experimental animals.", "content": "For the past 25 years, the research program of the Tulane University Department of Psychiatry and Neurology has been directed primarily to the development of treatment for patients with certain psychiatric and neurological disorders that have been resistant to commonly used therapy. In the course of investigations, using a variety of approaches, new techniques have evolved which have permitted simultaneous exploration of brain activity and behavior. The data reported substantiate an anatomical localization in the brain for the syndrome of psychotic behavior. Further, observations in patients, coupled with animal investigations, have led to the demonstration of brain pathways and previously undisclosed anatomical connections which provide a physical substrate for the clinically observed relation between perception and emotionality. These findings provide a basis for the development of specific biological methods for the treatment of behavioral disorders.", "contents": "Brain function and behavior. I. Emotion and sensory phenomena in psychotic patients and in experimental animals. For the past 25 years, the research program of the Tulane University Department of Psychiatry and Neurology has been directed primarily to the development of treatment for patients with certain psychiatric and neurological disorders that have been resistant to commonly used therapy. In the course of investigations, using a variety of approaches, new techniques have evolved which have permitted simultaneous exploration of brain activity and behavior. The data reported substantiate an anatomical localization in the brain for the syndrome of psychotic behavior. Further, observations in patients, coupled with animal investigations, have led to the demonstration of brain pathways and previously undisclosed anatomical connections which provide a physical substrate for the clinically observed relation between perception and emotionality. These findings provide a basis for the development of specific biological methods for the treatment of behavioral disorders."}
{"id": "PMID:1090710", "title": "Sexual functioning in patients with chronic renal failure.", "content": "There is little information on the sexual activity of patients with end-stage renal failure in spite of its being mentioned as a common side effect of kidney disease and chronic hemodialysis. In this report 32 married, male dialysis patients, 19 of whom had also received renal homografts, were interviewed and a detailed sexual history obtained. Twenty per cent of this population had no decrease in sexual functioning after the onset of kidney disease or the institution of dialysis, 45 per cent had reduced sexual potency after the onset of kidney disease, and another 35 per cent after beginning dialysis. Forty per cent of those patients receiving a kidney transplant had a subsequent increase in sexual potency.", "contents": "Sexual functioning in patients with chronic renal failure. There is little information on the sexual activity of patients with end-stage renal failure in spite of its being mentioned as a common side effect of kidney disease and chronic hemodialysis. In this report 32 married, male dialysis patients, 19 of whom had also received renal homografts, were interviewed and a detailed sexual history obtained. Twenty per cent of this population had no decrease in sexual functioning after the onset of kidney disease or the institution of dialysis, 45 per cent had reduced sexual potency after the onset of kidney disease, and another 35 per cent after beginning dialysis. Forty per cent of those patients receiving a kidney transplant had a subsequent increase in sexual potency."}
{"id": "PMID:1090714", "title": "Fat embolism: a comprehensive review.", "content": "Fat embolism as a cause or as a contributing factor to the death of injured patients is probably not diagnosed in many instances. Treatment is beneficial in moderate cases of fat at embolism; severe cases result in death and mild cases usually resolve without treatment. Awarness of the symptoms is important. Treatment should be directed to the symptoms rather than to the disease. Respiratory support seems to be the most effective treatment.", "contents": "Fat embolism: a comprehensive review. Fat embolism as a cause or as a contributing factor to the death of injured patients is probably not diagnosed in many instances. Treatment is beneficial in moderate cases of fat at embolism; severe cases result in death and mild cases usually resolve without treatment. Awarness of the symptoms is important. Treatment should be directed to the symptoms rather than to the disease. Respiratory support seems to be the most effective treatment."}
{"id": "PMID:1090717", "title": "Development of infectivity by the Plasmodium berghei sporozoite.", "content": "Studies were done on the development of infectivity during ontogeny of the sporozoite of the rodent malaria parasite, Plasmodium berghei. Populations of sporozoites were separated from the oocysts, the hemocoel, and the salivary glands, with special precautions being taken to avoid cross-contamination between the different populations. The results indicated that populations of salivary gland sporozoites were more than 10,000 times as infective as populations of oocyst sporozoites from the same mosquitoes. The development of this infectivity appears to be asynchronous, in some cases taking place in the hemocoel, while in other cases not occurring until after the sporozoites have invaded the salivary glands. Thus, the development of infectivity seems to be time-dependent rather than site-dependent. There is also a continued increase in sporozoite infectivity during their residence in the salivary gland. The development of infectivity may be associated with other aspects of sporozoite maturation, including changes in their antigenicity and motility.", "contents": "Development of infectivity by the Plasmodium berghei sporozoite. Studies were done on the development of infectivity during ontogeny of the sporozoite of the rodent malaria parasite, Plasmodium berghei. Populations of sporozoites were separated from the oocysts, the hemocoel, and the salivary glands, with special precautions being taken to avoid cross-contamination between the different populations. The results indicated that populations of salivary gland sporozoites were more than 10,000 times as infective as populations of oocyst sporozoites from the same mosquitoes. The development of this infectivity appears to be asynchronous, in some cases taking place in the hemocoel, while in other cases not occurring until after the sporozoites have invaded the salivary glands. Thus, the development of infectivity seems to be time-dependent rather than site-dependent. There is also a continued increase in sporozoite infectivity during their residence in the salivary gland. The development of infectivity may be associated with other aspects of sporozoite maturation, including changes in their antigenicity and motility."}
{"id": "PMID:1090718", "title": "A controlled trial of intraperitoneal cephaloridine administration in peritonitis.", "content": "From a controlled therapeutic trial extending for more than 2 yr and involving 69 patients with appendicitis and peritonitis a clear-cut statistically significant result emerged. There was a major reduction in the incidence of intraperitoneal abscesses using cephaloridine by the intraperitoneal as opposed to the systemic route. After randomized selection into treatment and control groups, cephaloridine, 25 mg/kg was given by injection every 6 hr for 48 hr to the treatment group by i.p. installation and to the control group by systemic injection. Both groups received initial intraoperative peritoneal lavage with normal saline and also continued systemic injections of cephaloridine on postoperative days 3, 4, and 5. Only one out of 36 patients in the treatment group developed a residual intraperitoneal abscess, as opposed to six abscesses developing in 33 patients in the control group. Technical problems and complications of the method were trivial and have not prevented us from continuing and extending the applications of the method.", "contents": "A controlled trial of intraperitoneal cephaloridine administration in peritonitis. From a controlled therapeutic trial extending for more than 2 yr and involving 69 patients with appendicitis and peritonitis a clear-cut statistically significant result emerged. There was a major reduction in the incidence of intraperitoneal abscesses using cephaloridine by the intraperitoneal as opposed to the systemic route. After randomized selection into treatment and control groups, cephaloridine, 25 mg/kg was given by injection every 6 hr for 48 hr to the treatment group by i.p. installation and to the control group by systemic injection. Both groups received initial intraoperative peritoneal lavage with normal saline and also continued systemic injections of cephaloridine on postoperative days 3, 4, and 5. Only one out of 36 patients in the treatment group developed a residual intraperitoneal abscess, as opposed to six abscesses developing in 33 patients in the control group. Technical problems and complications of the method were trivial and have not prevented us from continuing and extending the applications of the method."}
{"id": "PMID:1090719", "title": "Hemodialysis and renal transplantation in childhood: description of a program.", "content": "Over a 2-yr period eight kidneys have been transplanted in seven patients at the University Children's Hospital of Berne. Six of these patients are alive 4-24 mo after transplantation. The results seem encouraging and the procedure justified, especially in view of the chance of full rehabilitation, which is obviously less complete with chronic hemodialysis.", "contents": "Hemodialysis and renal transplantation in childhood: description of a program. Over a 2-yr period eight kidneys have been transplanted in seven patients at the University Children's Hospital of Berne. Six of these patients are alive 4-24 mo after transplantation. The results seem encouraging and the procedure justified, especially in view of the chance of full rehabilitation, which is obviously less complete with chronic hemodialysis."}
{"id": "PMID:1090720", "title": "The effects of two ultrasonic instruments on root surfaces.", "content": "The Orbison ultrasonic instrument and the Cavitron ultrasonic dental unit were compared for the smoothness of the root surfaces they produced. Twenty teeth were analyzed with the scanning electron microscope and classified as to rough or smooth. Both instruments produced similar degrees of smoothness on the root surfaces with minimal scarring. Application of these instruments to the root surface apical to the epithelial attachment produced residual smearing of some of the collagenous fibrils.", "contents": "The effects of two ultrasonic instruments on root surfaces. The Orbison ultrasonic instrument and the Cavitron ultrasonic dental unit were compared for the smoothness of the root surfaces they produced. Twenty teeth were analyzed with the scanning electron microscope and classified as to rough or smooth. Both instruments produced similar degrees of smoothness on the root surfaces with minimal scarring. Application of these instruments to the root surface apical to the epithelial attachment produced residual smearing of some of the collagenous fibrils."}
{"id": "PMID:1090722", "title": "The subcutaneous implantation of xenogeneic decalcified teeth.", "content": "Decalcified matrices obtained from bone and dentin have been used in xenogeneic implantation experiments. It is observed that such implants generally do not induce new bone but in many cases result in recalcification of old matrices. Treatment of the matrices with the enzyme inhibitor, iodoacetic acid, prior to implantation also did not enhance or improve the osteoinductive potentiality of these matrices. It is therefore suggested that the bone morphogenetic protein (BMP) to which the osteoinductive potentiality has been attributed by Urist et al. is a highly species-specific moiety and that the xenogeneic implant is hence a poor choice for its determination.", "contents": "The subcutaneous implantation of xenogeneic decalcified teeth. Decalcified matrices obtained from bone and dentin have been used in xenogeneic implantation experiments. It is observed that such implants generally do not induce new bone but in many cases result in recalcification of old matrices. Treatment of the matrices with the enzyme inhibitor, iodoacetic acid, prior to implantation also did not enhance or improve the osteoinductive potentiality of these matrices. It is therefore suggested that the bone morphogenetic protein (BMP) to which the osteoinductive potentiality has been attributed by Urist et al. is a highly species-specific moiety and that the xenogeneic implant is hence a poor choice for its determination."}
{"id": "PMID:1090726", "title": "A diagnostic wax-up technique.", "content": "A procedural outline has been presented for the accomplishment of a diagnostic wax-up as a preliminary step to actual preparation of the teeth as suggested by the proponents of gnathologic techniques. 8.9) The use of this technique will decrease the possibility of error in the construction of any extensive fixed partial denture prosthesis. The success of any procedure required the willingness of general dentists and specialists to, 'Spend more time planning than doing.'", "contents": "A diagnostic wax-up technique. A procedural outline has been presented for the accomplishment of a diagnostic wax-up as a preliminary step to actual preparation of the teeth as suggested by the proponents of gnathologic techniques. 8.9) The use of this technique will decrease the possibility of error in the construction of any extensive fixed partial denture prosthesis. The success of any procedure required the willingness of general dentists and specialists to, 'Spend more time planning than doing.'"}
{"id": "PMID:1090727", "title": "Factors affecting airborne beryllium concentrations in dental spaces.", "content": "Air sampling for beryllium concentrations produced during finishing procedures for a beryllium-containing alloy was conducted in two rooms with capacities of 700 and  10,000 cubic feet. The clearance rate of beryllium in the air and the effect of ventilation and room size on these  concentrations were investigated. With local lathe ventilation, no beryllium was found. Without local lathe ventilation, mean  10 minutes concentrations of about 23 mug per cubic meter were found at the breathing zone of the lathe operator in both rooms. At 4 and 8 feet from the breathing zone, sizable concentrations of beryllium above the maximum acceptable standard were found only in the small room. These levels decreased to zero 10 minutes after completion of the finishing and polishing procedure. It was concluded that there was little hazard to dental personnel when local lathe ventilation was used; however, our finding of high concentrations of beryllium in the air when lathe ventilation was not used indicates that continued vigilance must be maintained.", "contents": "Factors affecting airborne beryllium concentrations in dental spaces. Air sampling for beryllium concentrations produced during finishing procedures for a beryllium-containing alloy was conducted in two rooms with capacities of 700 and  10,000 cubic feet. The clearance rate of beryllium in the air and the effect of ventilation and room size on these  concentrations were investigated. With local lathe ventilation, no beryllium was found. Without local lathe ventilation, mean  10 minutes concentrations of about 23 mug per cubic meter were found at the breathing zone of the lathe operator in both rooms. At 4 and 8 feet from the breathing zone, sizable concentrations of beryllium above the maximum acceptable standard were found only in the small room. These levels decreased to zero 10 minutes after completion of the finishing and polishing procedure. It was concluded that there was little hazard to dental personnel when local lathe ventilation was used; however, our finding of high concentrations of beryllium in the air when lathe ventilation was not used indicates that continued vigilance must be maintained."}
{"id": "PMID:1090728", "title": "Modified hygroscopic gold casting technique.", "content": "A  modified hygrosopic gold casting technique has been described. It utilizes a dual-wax pattern with controlled amounts of water added to the setting investment to better influence both the location and amount of investment expansion. This technique has been used since 1970 with excellent clinical results.", "contents": "Modified hygroscopic gold casting technique. A  modified hygrosopic gold casting technique has been described. It utilizes a dual-wax pattern with controlled amounts of water added to the setting investment to better influence both the location and amount of investment expansion. This technique has been used since 1970 with excellent clinical results."}
{"id": "PMID:1090732", "title": "An impression technique for cast restorations.", "content": "A technique has been described for making impressions for cast restorations. It can be used for a single preparation or for multiple preparations in a single arch. The most unique feature of the teachnique is that the tray is formed over the prepared teeth, either directly or indirectly. The impression material is confined to a relatively small area and is forced by positive pressure into the gingival sulci around the prepared teeth. The result is an impression that is dense and void free and which records marginal and coronal surfaces with accurate detail. A dentist who is trained and experienced in the use of tray techniques can easily make the transition to this method.", "contents": "An impression technique for cast restorations. A technique has been described for making impressions for cast restorations. It can be used for a single preparation or for multiple preparations in a single arch. The most unique feature of the teachnique is that the tray is formed over the prepared teeth, either directly or indirectly. The impression material is confined to a relatively small area and is forced by positive pressure into the gingival sulci around the prepared teeth. The result is an impression that is dense and void free and which records marginal and coronal surfaces with accurate detail. A dentist who is trained and experienced in the use of tray techniques can easily make the transition to this method."}
{"id": "PMID:1090736", "title": "A macromolecule-free partially defined medium for Trypanosoma cruzi.", "content": "A macromolecule-free medium, containing in its defined part 3 salts, glucose, hemin, 21 amino acids, 3 lipids, and some undefined components obtained by dialysis of liver infusion, was developed for serial cultivation of Trypanosoma cruzi at 28 C.  The medium allows prolonged cultivation of T. cruzi by serial transfers and growth comparable to that obtained in more complex media, including those containing blood serum.", "contents": "A macromolecule-free partially defined medium for Trypanosoma cruzi. A macromolecule-free medium, containing in its defined part 3 salts, glucose, hemin, 21 amino acids, 3 lipids, and some undefined components obtained by dialysis of liver infusion, was developed for serial cultivation of Trypanosoma cruzi at 28 C.  The medium allows prolonged cultivation of T. cruzi by serial transfers and growth comparable to that obtained in more complex media, including those containing blood serum."}
{"id": "PMID:1090737", "title": "Three centuries of protozoology: a brief tribute to its founding father, A. van Leeuwenhoek of Delft.", "content": "It was exactly 300 years ago this month (August 1974) that the 17th century modest draper from Delft, Holland--Antony van Leeuwenhoek--discovered protozoa.  Describing them, often with amazing accuracy considering the optical equipment he was using (simply a home-made \"glorified\" hand lens), in letters to the Royal Society of London, he established himself, certainly, as the founding father of protozoology.  It is particularly appropriate for an assemblage of protozoologists to pay homage to this intrepid \"philosopher in little things,\" a man with an insatiable curiosity about his wee animalcules, on the tricentenary of his discovery of them, since it was an event of such long-lasting significance.", "contents": "Three centuries of protozoology: a brief tribute to its founding father, A. van Leeuwenhoek of Delft. It was exactly 300 years ago this month (August 1974) that the 17th century modest draper from Delft, Holland--Antony van Leeuwenhoek--discovered protozoa.  Describing them, often with amazing accuracy considering the optical equipment he was using (simply a home-made \"glorified\" hand lens), in letters to the Royal Society of London, he established himself, certainly, as the founding father of protozoology.  It is particularly appropriate for an assemblage of protozoologists to pay homage to this intrepid \"philosopher in little things,\" a man with an insatiable curiosity about his wee animalcules, on the tricentenary of his discovery of them, since it was an event of such long-lasting significance."}
{"id": "PMID:1090740", "title": "Antibodies and sperm survival in the female tract of the mouse and rabbit.", "content": "Rabbit and mouse spermatozoa from male and female tracts have been examined for their species-antigenic surface character, and for adherent antibodies, by double immunofluorescence techniques. Mouse spermatozoa from the ductus deferens showed an area over the acrosome which was positive to anti-mouse serum that had been absorbed with some male mouse somatic tissues including blood, but those from the uterus and oviduct were not stained. Spermatozoa from the uterus were shown to have an antibody coat on the acrosome, with anti-mouse IgG, but those from the ductus deferens and oviduct did not. Rabbit spermatozoa were more variable but their activity was similar: ejaculated spermatozoa sometimes already had antibody of male origin; the majority of the spermatozoa arriving early in the uterus were coated, but in general those that attained the oviducts were not coated. The results are interpreted as evidence for selection by the female tract of a small antigenically different population; the majority of spermatozoa are rejected and/or destroyed.", "contents": "Antibodies and sperm survival in the female tract of the mouse and rabbit. Rabbit and mouse spermatozoa from male and female tracts have been examined for their species-antigenic surface character, and for adherent antibodies, by double immunofluorescence techniques. Mouse spermatozoa from the ductus deferens showed an area over the acrosome which was positive to anti-mouse serum that had been absorbed with some male mouse somatic tissues including blood, but those from the uterus and oviduct were not stained. Spermatozoa from the uterus were shown to have an antibody coat on the acrosome, with anti-mouse IgG, but those from the ductus deferens and oviduct did not. Rabbit spermatozoa were more variable but their activity was similar: ejaculated spermatozoa sometimes already had antibody of male origin; the majority of the spermatozoa arriving early in the uterus were coated, but in general those that attained the oviducts were not coated. The results are interpreted as evidence for selection by the female tract of a small antigenically different population; the majority of spermatozoa are rejected and/or destroyed."}
{"id": "PMID:1090741", "title": "Cervicectomy with uterovaginal anastomosis in the rhesus monkey: a new surgical model for reproductive studies.", "content": "Cervicectomy with uterovaginal anastomosis in the rhesus monkey allows easy and non-traumatic access to the uterine cavity through the vagina for such purposes as biopsy, flushing, aspiration of fluid, and placement of IUDs. Endometrial and tissue samples cannot be contaminated by cervical mucus. The surgical procedure and results on eleven animals are described. Neither uterine nor ovarian function appeared to be significantly altered. The surgical model seems well suited to long-term studies on IUDs.", "contents": "Cervicectomy with uterovaginal anastomosis in the rhesus monkey: a new surgical model for reproductive studies. Cervicectomy with uterovaginal anastomosis in the rhesus monkey allows easy and non-traumatic access to the uterine cavity through the vagina for such purposes as biopsy, flushing, aspiration of fluid, and placement of IUDs. Endometrial and tissue samples cannot be contaminated by cervical mucus. The surgical procedure and results on eleven animals are described. Neither uterine nor ovarian function appeared to be significantly altered. The surgical model seems well suited to long-term studies on IUDs."}
{"id": "PMID:1090742", "title": "Progesterone: sites of action in inhibiting ovulation in hamsters.", "content": "A single subcutaneous injection of progesterone (0.5 mg/animal at 17.00 hours) on Day 3 of the cycle (Day 1 = day of vaginal discharge) interfered with ovulation and external vaginal discharge in all the animals. Lower doses were only partly active. Intravenous administration of LH, FSH or their combination at 14.00 hours on Day 4 failed to restore ovulation in such animals, implying a direct action of the administered progesterone on the ovary. A large dose of oestradiol benzoate (400 mug/animal) also proved inactive suggesting that progesterone did not interfere with ovulation solely through antioestrogenic action. Depletion of the pituitary LH stores normally found in association with ovulation and ovulatory peak of LH in plasma seen in control animals were not observed in the progesterone-treated hamsters suggesting a further action of progesterone at the pituitary-hypothalamic level in inhibiting ovulation. Administration of synthetic LRF (100 ng/animal, i.v.) on Day 4 to hamsters treated with progesterone caused an increase in plasma LH 15 min later which was comparable to the rise observed in control animals given a similar injection of LH-RF, implying lack of action of progesterone at the pituitary level. It is concluded that progesterone acts centrally, principally on the hypothalamus, and peripherally on the ovary to interfere with ovulation in hamsters. It is possible that the anovulatory state accompanying pregnancy results from these actions.", "contents": "Progesterone: sites of action in inhibiting ovulation in hamsters. A single subcutaneous injection of progesterone (0.5 mg/animal at 17.00 hours) on Day 3 of the cycle (Day 1 = day of vaginal discharge) interfered with ovulation and external vaginal discharge in all the animals. Lower doses were only partly active. Intravenous administration of LH, FSH or their combination at 14.00 hours on Day 4 failed to restore ovulation in such animals, implying a direct action of the administered progesterone on the ovary. A large dose of oestradiol benzoate (400 mug/animal) also proved inactive suggesting that progesterone did not interfere with ovulation solely through antioestrogenic action. Depletion of the pituitary LH stores normally found in association with ovulation and ovulatory peak of LH in plasma seen in control animals were not observed in the progesterone-treated hamsters suggesting a further action of progesterone at the pituitary-hypothalamic level in inhibiting ovulation. Administration of synthetic LRF (100 ng/animal, i.v.) on Day 4 to hamsters treated with progesterone caused an increase in plasma LH 15 min later which was comparable to the rise observed in control animals given a similar injection of LH-RF, implying lack of action of progesterone at the pituitary level. It is concluded that progesterone acts centrally, principally on the hypothalamus, and peripherally on the ovary to interfere with ovulation in hamsters. It is possible that the anovulatory state accompanying pregnancy results from these actions."}
{"id": "PMID:1090743", "title": "The burn wound from the surgical point of view.", "content": "The surgical approach cannot, and should not, be different for a mechanical wound and for a burn wound. Thin autografts applied over an excised burn wound act as the best antibiotic and the best analgetic, and at the same time provide a definite cover. The removal of thin skin grafts does not represent any additional danger to the burned patient. Homografts applied on excised dermal surfaces act as the best protection for potentially damaged layers of dermis, assuring spontaneous epithelization after their rejection. The clinical results obtained by primary necrectomies in extensive deep burns justify the conclusion that more of our effects should be concentrated towards an earlier and more adequate treatment in the early postburn phase. I am sure that the only chance of survival of critically burned patients depends on efficient action. Like Evans, I do believe in the value of whole blood transfusion in badly burned patients. We have to try to save their lives by changing their blood, as we do without hesitation in cases of badly matched blood transfusions.", "contents": "The burn wound from the surgical point of view. The surgical approach cannot, and should not, be different for a mechanical wound and for a burn wound. Thin autografts applied over an excised burn wound act as the best antibiotic and the best analgetic, and at the same time provide a definite cover. The removal of thin skin grafts does not represent any additional danger to the burned patient. Homografts applied on excised dermal surfaces act as the best protection for potentially damaged layers of dermis, assuring spontaneous epithelization after their rejection. The clinical results obtained by primary necrectomies in extensive deep burns justify the conclusion that more of our effects should be concentrated towards an earlier and more adequate treatment in the early postburn phase. I am sure that the only chance of survival of critically burned patients depends on efficient action. Like Evans, I do believe in the value of whole blood transfusion in badly burned patients. We have to try to save their lives by changing their blood, as we do without hesitation in cases of badly matched blood transfusions."}
{"id": "PMID:1090747", "title": "Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.", "content": "A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased.", "contents": "Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase. A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased."}
{"id": "PMID:1090748", "title": "Occurrence of the bacteriophage lambda receptor in some enterobacteriaceae.", "content": "In Escherichia coli K-12, the receptor for phage lambda is an outer membrane protein which inactivates the phage in vitro. Lambda receptor activity was found in extracts from all wild strains of E. coli tested, although most of them fail to support growth of the phage. In some cases this failure is due to a masking of the receptor in vivo, the bacteria being unable to adsorb the phage or to react with antireceptor antibodies. In other cases, adsorption does occur, and the nature of the block in phage growth was not investigated. Most Mal+ strains of Shigella have lambda receptor, whereas most Mal- strains do not have it. Synthesis of the lambda receptor in Shigella is thus presumably controlled by the positive regulator gene of the maltose regulon as is the case in E. coli K-12. Phage lambda adsorbs on many Mal+ strains of Shigella and even yields plaques on some of them, although at a low frequency. No lambda receptor activity could be found in extracts of several strains of Salmonella and Levinea.", "contents": "Occurrence of the bacteriophage lambda receptor in some enterobacteriaceae. In Escherichia coli K-12, the receptor for phage lambda is an outer membrane protein which inactivates the phage in vitro. Lambda receptor activity was found in extracts from all wild strains of E. coli tested, although most of them fail to support growth of the phage. In some cases this failure is due to a masking of the receptor in vivo, the bacteria being unable to adsorb the phage or to react with antireceptor antibodies. In other cases, adsorption does occur, and the nature of the block in phage growth was not investigated. Most Mal+ strains of Shigella have lambda receptor, whereas most Mal- strains do not have it. Synthesis of the lambda receptor in Shigella is thus presumably controlled by the positive regulator gene of the maltose regulon as is the case in E. coli K-12. Phage lambda adsorbs on many Mal+ strains of Shigella and even yields plaques on some of them, although at a low frequency. No lambda receptor activity could be found in extracts of several strains of Salmonella and Levinea."}
{"id": "PMID:1090749", "title": "Growth studies of three phi chi 174 mutants in tsDNA mutants Escherichia coli.", "content": "Three mutants of phi chi 174 were examined for their abilities to grow in temperature-sensitive dna,A, dnaC, dnaE, or dnaG mutants of Escherichia coli. The results indicate that the phage mutants have acquired the ability to grow in some tsDNA mutants that normally block the replication of wild-type phi chi 174. Evidence is presented indicating that the phage mutants contain one or more altered structural proteins. Several models are presented to explain how altered phage structural proteins could affect phi chi 174 replication.", "contents": "Growth studies of three phi chi 174 mutants in tsDNA mutants Escherichia coli. Three mutants of phi chi 174 were examined for their abilities to grow in temperature-sensitive dna,A, dnaC, dnaE, or dnaG mutants of Escherichia coli. The results indicate that the phage mutants have acquired the ability to grow in some tsDNA mutants that normally block the replication of wild-type phi chi 174. Evidence is presented indicating that the phage mutants contain one or more altered structural proteins. Several models are presented to explain how altered phage structural proteins could affect phi chi 174 replication."}
{"id": "PMID:1090750", "title": "Bacteriophage-host interaction and restriction of nonglucosylated T6.", "content": "Nonglucosylated T6 phage (T6gtam 16am30, hereafter called T6alpha gt-) were found to have two structural anomalies when compared with wild-type T6. The DNA of T6alpha gt- phage contains single-strand interruptions. These can be seen both during infection, in the pool of replicating DNA, and in DNA extracted from purified phage. In addition, the sodium dodecyl sulfate-polyacrylamide gel pattern of T6alpha gt- phage structural proteins reveals a protein band not found in T6. The altered protein has a mobility slightly faster than that of the major head protein, and it is not removed by osmotic shock. The restriction activity of Escherichia coli B directed against T6alpha gt- phage is abolished by preinfection of the cells for 4 min with T4 im m2. The shut-off of restriction is observed either by the rescue of superinfecting T6alpha gt- or by the failure to detect degradation of incoming T6alpha gt- DNA. This effect is resistant to rifampin and chloramphenicol.", "contents": "Bacteriophage-host interaction and restriction of nonglucosylated T6. Nonglucosylated T6 phage (T6gtam 16am30, hereafter called T6alpha gt-) were found to have two structural anomalies when compared with wild-type T6. The DNA of T6alpha gt- phage contains single-strand interruptions. These can be seen both during infection, in the pool of replicating DNA, and in DNA extracted from purified phage. In addition, the sodium dodecyl sulfate-polyacrylamide gel pattern of T6alpha gt- phage structural proteins reveals a protein band not found in T6. The altered protein has a mobility slightly faster than that of the major head protein, and it is not removed by osmotic shock. The restriction activity of Escherichia coli B directed against T6alpha gt- phage is abolished by preinfection of the cells for 4 min with T4 im m2. The shut-off of restriction is observed either by the rescue of superinfecting T6alpha gt- or by the failure to detect degradation of incoming T6alpha gt- DNA. This effect is resistant to rifampin and chloramphenicol."}
{"id": "PMID:1090751", "title": "Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. II. Location of the structural gene for thymidine kinase.", "content": "Amber mutants of bacteriophage T4 have been isolated that induce thymidine kinase activity only after infection of a strain of Escherichia coli carrying a suppressor mutation. The activity induced when one of these mutants infected this suppressor strain is much more heat sensitive than the activity induced by wild-type T4. This indicates that this amber mutation lies within the structural gene for thymidine kinase. This gene is between fI and v on the standard T4 genetic map. A mutant of tt4 that is unable to induce thymidine kinase activity incorporates only about one-eighth as much thymidine into its DNA as phage that do induce thymidine kinase. This contrasts to the findings that the total thymidine kinase activity in extracts prepared from cells infected with phage able to induce thymidine kinase in only twice as great as the activity in cells infected with the mutant unable to induce the enzyme.", "contents": "Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. II. Location of the structural gene for thymidine kinase. Amber mutants of bacteriophage T4 have been isolated that induce thymidine kinase activity only after infection of a strain of Escherichia coli carrying a suppressor mutation. The activity induced when one of these mutants infected this suppressor strain is much more heat sensitive than the activity induced by wild-type T4. This indicates that this amber mutation lies within the structural gene for thymidine kinase. This gene is between fI and v on the standard T4 genetic map. A mutant of tt4 that is unable to induce thymidine kinase activity incorporates only about one-eighth as much thymidine into its DNA as phage that do induce thymidine kinase. This contrasts to the findings that the total thymidine kinase activity in extracts prepared from cells infected with phage able to induce thymidine kinase in only twice as great as the activity in cells infected with the mutant unable to induce the enzyme."}
{"id": "PMID:1090752", "title": "Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B.", "content": "A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-specific antigens and infectious virus. By contrast, JE failed to replicate or produce virus-specific antigen in cells which had been enucleated before or even 2 h post infection. Studies of the effect of enulceation at various times after infection demonstrated that a nucleus must be present at least 2 and possibly as long as 4 h after infection to produce either JE-specific antigen or infectious JE virus. These studies demonstrate that the replication of SIN, a group A arbovirus (togavirus), which has no nuclear requirement, contrasts sharply with that of a group B arbovirus (togavirus), JE, which may have an initial dependence on a nucleus-associated process.", "contents": "Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B. A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-specific antigens and infectious virus. By contrast, JE failed to replicate or produce virus-specific antigen in cells which had been enucleated before or even 2 h post infection. Studies of the effect of enulceation at various times after infection demonstrated that a nucleus must be present at least 2 and possibly as long as 4 h after infection to produce either JE-specific antigen or infectious JE virus. These studies demonstrate that the replication of SIN, a group A arbovirus (togavirus), which has no nuclear requirement, contrasts sharply with that of a group B arbovirus (togavirus), JE, which may have an initial dependence on a nucleus-associated process."}
{"id": "PMID:1090753", "title": "Characterization of new regulatory mutants of bacteriophage T4. II. New class of mutants.", "content": "New mutants of bacteriophage T4 that overproduce the enzyme dihydrofolate reductase were investigated. Unlike previously described overproducers of this enzyme (Johnson and Hall, 1974), these mutants did not overproduce deoxycytidylate deaminase. Overproduction of dihydrofolate reductase by the new mutants occurred because enzymatic activity continued to increase for a longer period of time in cells infected by the mutants than in cells infected by wild-type phage. This continued increase occurred even in the presence of rifampin, indicating that the overproduction is probably due to a post-transcriptional event. Both these new overproducers and the previously  described overproducers were studied by using polyacrylamide gel electrophoresis. The two types of overproducers appeared to be very different. The previously described overproducers showed a delay and/or reduction in the synthesis of several proteins that normally started to be made 4 to 6 min after infection. Several proteins could be seen to be overproduced on the gels. The new overproducers did not show the delay in the synthesis of some proteins and only overproduced a few proteins. The new gene defined by the new overproducers is between the gene coding for thymidine kinase and the gene coding for lysozyme.", "contents": "Characterization of new regulatory mutants of bacteriophage T4. II. New class of mutants. New mutants of bacteriophage T4 that overproduce the enzyme dihydrofolate reductase were investigated. Unlike previously described overproducers of this enzyme (Johnson and Hall, 1974), these mutants did not overproduce deoxycytidylate deaminase. Overproduction of dihydrofolate reductase by the new mutants occurred because enzymatic activity continued to increase for a longer period of time in cells infected by the mutants than in cells infected by wild-type phage. This continued increase occurred even in the presence of rifampin, indicating that the overproduction is probably due to a post-transcriptional event. Both these new overproducers and the previously  described overproducers were studied by using polyacrylamide gel electrophoresis. The two types of overproducers appeared to be very different. The previously described overproducers showed a delay and/or reduction in the synthesis of several proteins that normally started to be made 4 to 6 min after infection. Several proteins could be seen to be overproduced on the gels. The new overproducers did not show the delay in the synthesis of some proteins and only overproduced a few proteins. The new gene defined by the new overproducers is between the gene coding for thymidine kinase and the gene coding for lysozyme."}
{"id": "PMID:1090754", "title": "Escherichia coli capsule bacteriophages. III. Fragments of bacteriophage 29.", "content": "A glycanase activity, catalyzing the depolymerization of host capsular polysaccharide, is associated with Escherichia coli capsule bacteriophage no. 29, a small virus with an isometric head, carrying a base plate with a set of spikes. The bacteriophage particles were disrupted by mild acid treatment (5 to 8 min at pH 3.5 and 37 C), and the enzymatically active fragments were isolated and subjected to sodium dodecyl sulfate-gel electrophoresis as well as to electron microscopy. Of the at least nine different polypeptide chains found in the complete virion, three (of 57,000 plus or minus 3,000, 29,500 plus or minus 2,000 and 13,500 plus or minus 1,000 daltons) were detected in detached base plates. They had the appearance of six-pointed stars of about 14 nm in outer diameter, with a central hole or prop, carrying six (or, possibly, a multiple thereof) spikes. Two sizes of polypeptide chains (57,000 and 29,500) were found in pure spikes, cylindrical particles of about 14.5 to 15 nm in length and 5 nm in diameter, and one (57,000) in -- still capsule depolymerizing -- spike subunits of roughly 5 nm in diameter. Phage 29 spike preparations, homogeneous in analytical ultracentrifugation and immunoelectrophoresis, were found to have a molecular weight of 245,000, as determined from the sedimentation equilibrium, and to contain equimolar amounts of the two polypeptides, probably three copies of each per organelle. The amino acid analysis of the isolated spikes revealed that aspartic acid, alanine, serine, and glycine are their dominant constituents; no amino sugars or other carbohydrates were detected in the preparations.", "contents": "Escherichia coli capsule bacteriophages. III. Fragments of bacteriophage 29. A glycanase activity, catalyzing the depolymerization of host capsular polysaccharide, is associated with Escherichia coli capsule bacteriophage no. 29, a small virus with an isometric head, carrying a base plate with a set of spikes. The bacteriophage particles were disrupted by mild acid treatment (5 to 8 min at pH 3.5 and 37 C), and the enzymatically active fragments were isolated and subjected to sodium dodecyl sulfate-gel electrophoresis as well as to electron microscopy. Of the at least nine different polypeptide chains found in the complete virion, three (of 57,000 plus or minus 3,000, 29,500 plus or minus 2,000 and 13,500 plus or minus 1,000 daltons) were detected in detached base plates. They had the appearance of six-pointed stars of about 14 nm in outer diameter, with a central hole or prop, carrying six (or, possibly, a multiple thereof) spikes. Two sizes of polypeptide chains (57,000 and 29,500) were found in pure spikes, cylindrical particles of about 14.5 to 15 nm in length and 5 nm in diameter, and one (57,000) in -- still capsule depolymerizing -- spike subunits of roughly 5 nm in diameter. Phage 29 spike preparations, homogeneous in analytical ultracentrifugation and immunoelectrophoresis, were found to have a molecular weight of 245,000, as determined from the sedimentation equilibrium, and to contain equimolar amounts of the two polypeptides, probably three copies of each per organelle. The amino acid analysis of the isolated spikes revealed that aspartic acid, alanine, serine, and glycine are their dominant constituents; no amino sugars or other carbohydrates were detected in the preparations."}
{"id": "PMID:1090755", "title": "Escherichia coli capsule bacteriophages. IV. Free capsule depolymerase 29.", "content": "The free host capsule depolymerase, induced by Escherichia coli capsule bacteriophage no. 29, and causing the formation of haloes around its plaques, has been purified to homogeneity. As judged from the following facts, this \"enzyme\" consists of free phage 29 spikes. (i) Detached phage organelles and depolymerase 29 particles exhibit the same molecular weight (about 245,000, as determined from the sedimentation equilibrium), contain polypeptide chains of the same two sizes (57,000 plus or minus 3,000 and 29,500 plus or minus 2,000, as determined by SDS-PAA gel electrophoresis), and have (within experimental error) the same sedimentation coefficient, isoelectric point, and amino acid composition. (ii) Isolated depolymerase and phage spikes in situ both catalyze the hydrolysis of glucosidic bonds in host capsular polysaccharide, leading ultimately to the formation of oligosaccharide fragments of one, two, and three hexasaccharide repeating units. (iii) Depolymerase 29 and phage 29 spikes have roughly the same electron optical dimensions. As tentatively estimated from the total and the virus-associated capsule depolymerase activity in the lysates, phage 29 infection seems to produce eight to seventeen times more free than incorporated spikes.", "contents": "Escherichia coli capsule bacteriophages. IV. Free capsule depolymerase 29. The free host capsule depolymerase, induced by Escherichia coli capsule bacteriophage no. 29, and causing the formation of haloes around its plaques, has been purified to homogeneity. As judged from the following facts, this \"enzyme\" consists of free phage 29 spikes. (i) Detached phage organelles and depolymerase 29 particles exhibit the same molecular weight (about 245,000, as determined from the sedimentation equilibrium), contain polypeptide chains of the same two sizes (57,000 plus or minus 3,000 and 29,500 plus or minus 2,000, as determined by SDS-PAA gel electrophoresis), and have (within experimental error) the same sedimentation coefficient, isoelectric point, and amino acid composition. (ii) Isolated depolymerase and phage spikes in situ both catalyze the hydrolysis of glucosidic bonds in host capsular polysaccharide, leading ultimately to the formation of oligosaccharide fragments of one, two, and three hexasaccharide repeating units. (iii) Depolymerase 29 and phage 29 spikes have roughly the same electron optical dimensions. As tentatively estimated from the total and the virus-associated capsule depolymerase activity in the lysates, phage 29 infection seems to produce eight to seventeen times more free than incorporated spikes."}
{"id": "PMID:1090756", "title": "Escherichia coli capsule bacteriophages. V. Lysozyme 29.", "content": "In addition to the spike-associated host capsule depolymerase, infection by Escherichia coli capsule bacteriophage no. 29 also induces the synthesis of a large bacteriolytic enzyme which has been purified to homogeneity. On incubation of isolated host murein sacculi with this enzyme, no amino groups but reducing sugar groups were liberated, and muraminitol, but no glucosaminitol, was found in the degraded sacculi after subsequent reduction with NaBH4. The bacteriolytic enzyme is thus another lysozyme (mucopeptide N-acetylmuramylhydrolase; EC 3.2.1.17). Electron optical visualization of negatively stained lysozyme specimens showed oblong particles of roughly 4.5 to 5.5 nm in diameter and 15 to 19 nm in length. Although the material tended to dissociate, a crude estimate of its molecular weight (270,000 plus or minus 30,000) could be obtained from these dimensions, from its sedimentation equilibrium, and from its behavior in gel chromatography. After disintegration of homogeneous lysozyme 29 by heating in solution with sodium dodecyl sulfate and dithiothreitol, polypeptides of one size only (about 46,000 dalton, probably six copies per molecule) were found in sodium dodecyl sulfate-polyacrylamide electrophoresis. The amino acid analysis of the enzyme accounted for more than 90% of its dry weight. One percent or less of the bacteriolytic activity in phage 29 lysates was found to be associated with the intact or disrupted virus particles, and a polypeptide of 46,000 daltons was not detected in the virions. These results strongly suggest that, in contrast to the host capsule depolymerase also induced by the same phage, and in spite of its comparatively large size, \"lysozyme 29\" does not constitute an integral part also of the homologous bacteriophage particles.", "contents": "Escherichia coli capsule bacteriophages. V. Lysozyme 29. In addition to the spike-associated host capsule depolymerase, infection by Escherichia coli capsule bacteriophage no. 29 also induces the synthesis of a large bacteriolytic enzyme which has been purified to homogeneity. On incubation of isolated host murein sacculi with this enzyme, no amino groups but reducing sugar groups were liberated, and muraminitol, but no glucosaminitol, was found in the degraded sacculi after subsequent reduction with NaBH4. The bacteriolytic enzyme is thus another lysozyme (mucopeptide N-acetylmuramylhydrolase; EC 3.2.1.17). Electron optical visualization of negatively stained lysozyme specimens showed oblong particles of roughly 4.5 to 5.5 nm in diameter and 15 to 19 nm in length. Although the material tended to dissociate, a crude estimate of its molecular weight (270,000 plus or minus 30,000) could be obtained from these dimensions, from its sedimentation equilibrium, and from its behavior in gel chromatography. After disintegration of homogeneous lysozyme 29 by heating in solution with sodium dodecyl sulfate and dithiothreitol, polypeptides of one size only (about 46,000 dalton, probably six copies per molecule) were found in sodium dodecyl sulfate-polyacrylamide electrophoresis. The amino acid analysis of the enzyme accounted for more than 90% of its dry weight. One percent or less of the bacteriolytic activity in phage 29 lysates was found to be associated with the intact or disrupted virus particles, and a polypeptide of 46,000 daltons was not detected in the virions. These results strongly suggest that, in contrast to the host capsule depolymerase also induced by the same phage, and in spite of its comparatively large size, \"lysozyme 29\" does not constitute an integral part also of the homologous bacteriophage particles."}
{"id": "PMID:1090757", "title": "Extravesical ureteroneocystostomy in 170 renal transplant patients.", "content": "Urinary tract reconstitution was done in 170 consecutive renal transplant patients, using an extravesical ureteronecystostomy. The urologic complication rate was 8.5 per cent but complications associated directly with the anastomosis occurred in only 5 per cent of the cases. There have been no anastomotic complications in the last 104 transplants. Vesicoureteral reflux occurred in 20 per cent of 50 patients surveyed. Death in 2 cases and loss of the kidney in 1 were associated with urologic complications.", "contents": "Extravesical ureteroneocystostomy in 170 renal transplant patients. Urinary tract reconstitution was done in 170 consecutive renal transplant patients, using an extravesical ureteronecystostomy. The urologic complication rate was 8.5 per cent but complications associated directly with the anastomosis occurred in only 5 per cent of the cases. There have been no anastomotic complications in the last 104 transplants. Vesicoureteral reflux occurred in 20 per cent of 50 patients surveyed. Death in 2 cases and loss of the kidney in 1 were associated with urologic complications."}
{"id": "PMID:1090758", "title": "Transverse colon conduit: a preferred method of urinary diversion for radiation-treated pelvic malignancies.", "content": "In the management of pelvic malignancies treated by radiation the standard ileal conduit is subject to many hazards related to the use of damaged tissues. The transverse colon affords the use of a short isolated segment of non-irradiated bowel as a urinary conduit. The ureters can be dissected well above the field of pelvic irradiation. Eight patients with bladder or cervical carcinoma treated with high doses of external radiotherapy are presented to demonstrate the usefulness of the transverse colon in supravesical urinary diversions.", "contents": "Transverse colon conduit: a preferred method of urinary diversion for radiation-treated pelvic malignancies. In the management of pelvic malignancies treated by radiation the standard ileal conduit is subject to many hazards related to the use of damaged tissues. The transverse colon affords the use of a short isolated segment of non-irradiated bowel as a urinary conduit. The ureters can be dissected well above the field of pelvic irradiation. Eight patients with bladder or cervical carcinoma treated with high doses of external radiotherapy are presented to demonstrate the usefulness of the transverse colon in supravesical urinary diversions."}
{"id": "PMID:1090759", "title": "The ileocecal bladder: a new method for urinary diversion after radical cystectomy (a preliminary report).", "content": "Ten patients underwent radical cystectomy and ileocecal bladder reconstruction for carcinoma of the bladder. The ileocecal region was used as an artificial bladder, its valve protecting the kidneys from back pressure exerted by the external urethral sphincter. The ureters were anastomosed to the ileal part and the cecum was anastomosed to the prostatic urethra in 7 men and to the bladder neck in 2 women and 1 man. Two patients died postoperatively. All patients had perfect control of micturition during the day. Those in whom the bladder neck was preserved were also fully continent at night. The remaining patients had a minor degree of nocturnal enuresis with only a few drops of urine voided involuntarily during sleep. All patients feel the desire to void when the bladder is full. Voiding cystograms in all patients did not reveal reflux in the ureters. IVP's done up to 11 months postoperatively showed preservation and improvement of kidney function and configuration. Followup cystometric studies and serum electrolyte determinations are under investigation.", "contents": "The ileocecal bladder: a new method for urinary diversion after radical cystectomy (a preliminary report). Ten patients underwent radical cystectomy and ileocecal bladder reconstruction for carcinoma of the bladder. The ileocecal region was used as an artificial bladder, its valve protecting the kidneys from back pressure exerted by the external urethral sphincter. The ureters were anastomosed to the ileal part and the cecum was anastomosed to the prostatic urethra in 7 men and to the bladder neck in 2 women and 1 man. Two patients died postoperatively. All patients had perfect control of micturition during the day. Those in whom the bladder neck was preserved were also fully continent at night. The remaining patients had a minor degree of nocturnal enuresis with only a few drops of urine voided involuntarily during sleep. All patients feel the desire to void when the bladder is full. Voiding cystograms in all patients did not reveal reflux in the ureters. IVP's done up to 11 months postoperatively showed preservation and improvement of kidney function and configuration. Followup cystometric studies and serum electrolyte determinations are under investigation."}
{"id": "PMID:1090760", "title": "Ileocecal conduit for temporary and permanent urinary diversion.", "content": "The ileocecal intestinal segment has been used as a diverting conduit with a satisfactory colonic stoma in 6 patients with potentially reversible bladder disorders. Followup has been from 1 to 5 years. The ileocecal valve has been modified successfully by a fundoplication procedure similar to the Nissen esophagogastric junction operation to prevent ileocecal and ureteral reflux. IVP and renal function studies revealed resolution of pre-existing hydronephrosis and preservation of previously normal upper urinary tracts. One patient has undergone reversal of the diversion by cecocystoplasty and simultaneous bladder augmentation, and has been followed for 5 years with sterile urine and normal IVPs. The anatomic and functional advantages of a conduit with an antireflux mechanism that is applicable to the hydronephrotic collecting system are discussed.", "contents": "Ileocecal conduit for temporary and permanent urinary diversion. The ileocecal intestinal segment has been used as a diverting conduit with a satisfactory colonic stoma in 6 patients with potentially reversible bladder disorders. Followup has been from 1 to 5 years. The ileocecal valve has been modified successfully by a fundoplication procedure similar to the Nissen esophagogastric junction operation to prevent ileocecal and ureteral reflux. IVP and renal function studies revealed resolution of pre-existing hydronephrosis and preservation of previously normal upper urinary tracts. One patient has undergone reversal of the diversion by cecocystoplasty and simultaneous bladder augmentation, and has been followed for 5 years with sterile urine and normal IVPs. The anatomic and functional advantages of a conduit with an antireflux mechanism that is applicable to the hydronephrotic collecting system are discussed."}
{"id": "PMID:1090762", "title": "Intermittent catheterization rather than urinary diversion in children with meningomyelocele.", "content": "Fifteen girls more than 4 years old and with neuromuscular defects of meningomyelocele are maintaining adequate urinary continence through the use of intraurethral catheterization done by themselves or their parents. Urinary diversion has been necessary in only 1 child. The indwelling catheter has allowed heavily trabeculated bladders to become smooth enough for an antirflux operation to be successful, although the period of followup is only 2 years. Upper tract deterioration has not been observed and urine has been maintained sterile without the help of medication 50 per cent of the time when catheterizations have been done on a routine 3-hour schedule. For this method to be sucessful and replace conduit deversion, parents, child, nurse and physician must be a well-knit team. With the hope that a reliable artificial urinary sphincter will eventually provide contience for some children, one is further justified in pursuing urinary continence by use of the catheter, instead of resorting early to permanent conduit conversion.", "contents": "Intermittent catheterization rather than urinary diversion in children with meningomyelocele. Fifteen girls more than 4 years old and with neuromuscular defects of meningomyelocele are maintaining adequate urinary continence through the use of intraurethral catheterization done by themselves or their parents. Urinary diversion has been necessary in only 1 child. The indwelling catheter has allowed heavily trabeculated bladders to become smooth enough for an antirflux operation to be successful, although the period of followup is only 2 years. Upper tract deterioration has not been observed and urine has been maintained sterile without the help of medication 50 per cent of the time when catheterizations have been done on a routine 3-hour schedule. For this method to be sucessful and replace conduit deversion, parents, child, nurse and physician must be a well-knit team. With the hope that a reliable artificial urinary sphincter will eventually provide contience for some children, one is further justified in pursuing urinary continence by use of the catheter, instead of resorting early to permanent conduit conversion."}
{"id": "PMID:1090763", "title": "Renal transplantation in cystinosis.", "content": "Three children from 6 1/2 to 10 years old received kidney allografts from their parents 2 1/2 to 4 1/2 years ago. Renal function has been stable and the patients have been doing well. Studies of renal tubular function as well as morphologic studies by light and electron microscopy, and microdissection of renal tubules fail to reveal evidence of recurrent disease in the allografted kidneys.", "contents": "Renal transplantation in cystinosis. Three children from 6 1/2 to 10 years old received kidney allografts from their parents 2 1/2 to 4 1/2 years ago. Renal function has been stable and the patients have been doing well. Studies of renal tubular function as well as morphologic studies by light and electron microscopy, and microdissection of renal tubules fail to reveal evidence of recurrent disease in the allografted kidneys."}
{"id": "PMID:1090764", "title": "Hyperosmolar nonketotic syndrome and steroid diabetes. Occurrence after renal transplantation.", "content": "So-called steroid (glucocorticoids) diabetes developed in 11 (5.5%) of 202 patients receiving 216 renal allografts between December 1963 and June 1974. In three of the 11 patients, hyperosmolar nonketotic syndrome was present at diagnosis; all of the three recovered. Eight of the 11 patients survived with functioning allografts, and only one of the eight requires insulin. Hyperglycemia has been detected in most transplant patients tested in the immediate postoperative period. Factors (including stress from surgery and treatment with drugs such as furosemide) predispose to this condition.", "contents": "Hyperosmolar nonketotic syndrome and steroid diabetes. Occurrence after renal transplantation. So-called steroid (glucocorticoids) diabetes developed in 11 (5.5%) of 202 patients receiving 216 renal allografts between December 1963 and June 1974. In three of the 11 patients, hyperosmolar nonketotic syndrome was present at diagnosis; all of the three recovered. Eight of the 11 patients survived with functioning allografts, and only one of the eight requires insulin. Hyperglycemia has been detected in most transplant patients tested in the immediate postoperative period. Factors (including stress from surgery and treatment with drugs such as furosemide) predispose to this condition."}
{"id": "PMID:1090766", "title": "T rosettes in alcoholic cirrhosis of the liver.", "content": "Thirty patients with alcoholic cirrhosis of the liver were studied for in vivo and in vitro correlates of cellular immunity. Seventy-seven percent failed to be sensitized to dinitrochlorobenzene, indicating impairment of the in vivo cellular immune response. A significant decrease in the number of T-rosette-forming cells was observed in this group of patients (.01 smaller than P smaller than .025). This finding suggests that the active T-rosette test is a valuable tool in detecting partial alterations in cell-mediated immunity in alcoholic cirrhosis of the liver. Our results also suggest that rosette formation is a more sensitive indicator of cell-mediated immunity than phytohemagglutinin-stimulated blastogenesis in patients with alcoholic cirrhosis of the liver.", "contents": "T rosettes in alcoholic cirrhosis of the liver. Thirty patients with alcoholic cirrhosis of the liver were studied for in vivo and in vitro correlates of cellular immunity. Seventy-seven percent failed to be sensitized to dinitrochlorobenzene, indicating impairment of the in vivo cellular immune response. A significant decrease in the number of T-rosette-forming cells was observed in this group of patients (.01 smaller than P smaller than .025). This finding suggests that the active T-rosette test is a valuable tool in detecting partial alterations in cell-mediated immunity in alcoholic cirrhosis of the liver. Our results also suggest that rosette formation is a more sensitive indicator of cell-mediated immunity than phytohemagglutinin-stimulated blastogenesis in patients with alcoholic cirrhosis of the liver."}
{"id": "PMID:1090767", "title": "Conventional coronary care unit monitoring. Nondetection of transient rhythm disturbances.", "content": "Conventional electrocardiographic monitoring techniques depend on human observation of an oscilloscopic display for detection of transient rhythm disturbances such as premature ventricular contractions (PVCs). It is not surprising that such events are often missed. We have found that transient events are regularly identified only when they occur with a frequency exceeding one per minute. Rhythm disturbances such as coupled PVCs or PVCs in which R waves interrupt the preceding T wave (\"R on T\"), which constitute indications for antiarrhythmic therapy, may go undetected if they occur less frequently than this.", "contents": "Conventional coronary care unit monitoring. Nondetection of transient rhythm disturbances. Conventional electrocardiographic monitoring techniques depend on human observation of an oscilloscopic display for detection of transient rhythm disturbances such as premature ventricular contractions (PVCs). It is not surprising that such events are often missed. We have found that transient events are regularly identified only when they occur with a frequency exceeding one per minute. Rhythm disturbances such as coupled PVCs or PVCs in which R waves interrupt the preceding T wave (\"R on T\"), which constitute indications for antiarrhythmic therapy, may go undetected if they occur less frequently than this."}
{"id": "PMID:1090770", "title": "Shiga bacillus dysentery associated with marked leukocytosis and erythrocyte fragmentation.", "content": "Granulocytic leukemoid reactions (white blood cell counts greater than 50,000 with myelocytes and promyelocytes in the peripheral blood) were documented in 15 per cent of 273 patients with dysentery due to Shigella dysenteriae, type 1 (Shiga bacillus) in Bangladesh. Peak granulocytosis occurred during the second week of illness, when the children were commonly afebrile and diarrhea had ceased or was subsiding. More than half of the patients with leukemoid reactions subsequently developed a fall in hematocrit associated with striking erythrocyte fragmentation on blood smears. Thrombocytopenia occurred during the period of hemolysis in most. Transient oliguric renal failure developed in several patients. Most made a complete recovery. The pathogenesis of the syndrome and the reason for its high incidence were not determined.", "contents": "Shiga bacillus dysentery associated with marked leukocytosis and erythrocyte fragmentation. Granulocytic leukemoid reactions (white blood cell counts greater than 50,000 with myelocytes and promyelocytes in the peripheral blood) were documented in 15 per cent of 273 patients with dysentery due to Shigella dysenteriae, type 1 (Shiga bacillus) in Bangladesh. Peak granulocytosis occurred during the second week of illness, when the children were commonly afebrile and diarrhea had ceased or was subsiding. More than half of the patients with leukemoid reactions subsequently developed a fall in hematocrit associated with striking erythrocyte fragmentation on blood smears. Thrombocytopenia occurred during the period of hemolysis in most. Transient oliguric renal failure developed in several patients. Most made a complete recovery. The pathogenesis of the syndrome and the reason for its high incidence were not determined."}
{"id": "PMID:1090776", "title": "[Analysis of the structure of cardiac rhythm in auricular fibrillation with the aid of a specialized computer].", "content": "In 37 patients with atrial fibrillation of varying etiology the electron-computer technique was used in recording the cardiointervalogram and interval histogram and also in determining a number of indices characterizing distinctive features of the R-R intervals distribution. When analyzing the available data not was taken of a tendency towards appearance of definite types of the interval histograms, variations in the mean level of the cardiointervalogram, resembling the so-called slow waves observable in the sinus rhythm. During repeated investigations made in the same patients features of a single type in the distribution of the R-R intervals were apparent. In the course of conservative therapy of atrial fibrillation a certain stereotypy in the R-R intervals distribution was noted, it being indicative of an increased arrhythmicity in the work of the heart occurring simultaneously with the slowing down of the cardiac contractions.", "contents": "[Analysis of the structure of cardiac rhythm in auricular fibrillation with the aid of a specialized computer]. In 37 patients with atrial fibrillation of varying etiology the electron-computer technique was used in recording the cardiointervalogram and interval histogram and also in determining a number of indices characterizing distinctive features of the R-R intervals distribution. When analyzing the available data not was taken of a tendency towards appearance of definite types of the interval histograms, variations in the mean level of the cardiointervalogram, resembling the so-called slow waves observable in the sinus rhythm. During repeated investigations made in the same patients features of a single type in the distribution of the R-R intervals were apparent. In the course of conservative therapy of atrial fibrillation a certain stereotypy in the R-R intervals distribution was noted, it being indicative of an increased arrhythmicity in the work of the heart occurring simultaneously with the slowing down of the cardiac contractions."}
{"id": "PMID:1090777", "title": "[Results of perforating keratoplasty in hereditary corneal diseases (author's transl)].", "content": "Operation results of keratoplasty in 50 eyes having keratoconus are presented and discusses. Furthermore a report is made on keratoplasty in cases of keratotorus in reticulate, maculate, and granulate dystrophies as well as in congenital ichthyosis with corneal involvement. The prognosis and complications of this intervention are presented.", "contents": "[Results of perforating keratoplasty in hereditary corneal diseases (author's transl)]. Operation results of keratoplasty in 50 eyes having keratoconus are presented and discusses. Furthermore a report is made on keratoplasty in cases of keratotorus in reticulate, maculate, and granulate dystrophies as well as in congenital ichthyosis with corneal involvement. The prognosis and complications of this intervention are presented."}
{"id": "PMID:1090784", "title": "Saccular aneurysm of ascending aorta caused by granulomatous aortitis in a child.", "content": "This report describes a 7-year-old boy who underwent successful surgical excision of a saccular aneurysm of the ascending aorta caused by giant cell aortitis. The aneurysm was removed by lateral aortorrhaphy without cardiopulmonary bypass. The clinical manifestations and appropriate surgical therapy of granulomatous aortitis in childhood are discussed.", "contents": "Saccular aneurysm of ascending aorta caused by granulomatous aortitis in a child. This report describes a 7-year-old boy who underwent successful surgical excision of a saccular aneurysm of the ascending aorta caused by giant cell aortitis. The aneurysm was removed by lateral aortorrhaphy without cardiopulmonary bypass. The clinical manifestations and appropriate surgical therapy of granulomatous aortitis in childhood are discussed."}
{"id": "PMID:1090785", "title": "Accumulation of 131I hippurate in rat heart allografts during rejection.", "content": "The accumulation of 131I hippurate in heterotopic (abdominal) rat heart allografts (Brown-Norway to Lewis) in comparison with native (autologous) hearts 6 and 7 days after transplantation was studied by noninvasive scintigraphic imaging and by direct isotope counting of excised hearts. Six hours after intravenous isotope injection, isotope accumulation in the allografted heart was clearly identified by scintigraphic study in comparison with the background, kidneys, urinary bladder, and native heart. Six and 7 days after transplantation, excised allografted hearts were significantly increased in weight in comparison with native hearts. A significant increase in isotope accumulation in allografted hearts in comparison with native hearts was found both per heart and per gram of heart. The findings of this study suggests the potential usefulness of 131I hippurate in the immunoinflammatory monitoring of organ allograft rejection by direct counting as well as by noninvasive scintigraphic study if sufficient time is allowed to permit clearance of high concentrations of isotope from the background areas, kidneys, and urinary bladder.", "contents": "Accumulation of 131I hippurate in rat heart allografts during rejection. The accumulation of 131I hippurate in heterotopic (abdominal) rat heart allografts (Brown-Norway to Lewis) in comparison with native (autologous) hearts 6 and 7 days after transplantation was studied by noninvasive scintigraphic imaging and by direct isotope counting of excised hearts. Six hours after intravenous isotope injection, isotope accumulation in the allografted heart was clearly identified by scintigraphic study in comparison with the background, kidneys, urinary bladder, and native heart. Six and 7 days after transplantation, excised allografted hearts were significantly increased in weight in comparison with native hearts. A significant increase in isotope accumulation in allografted hearts in comparison with native hearts was found both per heart and per gram of heart. The findings of this study suggests the potential usefulness of 131I hippurate in the immunoinflammatory monitoring of organ allograft rejection by direct counting as well as by noninvasive scintigraphic study if sufficient time is allowed to permit clearance of high concentrations of isotope from the background areas, kidneys, and urinary bladder."}
{"id": "PMID:1090786", "title": "Tracheal reconstruction with a prosthesis of marlex mesh and pericardium.", "content": "Tracheal reconstruction with a new type of prosthesis made of heavy Marlex mesh and pericardium is described in 13 patients suffering from malignancy. Two patients had circumferential excision of the trachea, and the remaining 11 had partial circumferential excision and a patch graft. In 10 of them, tracheal excision was a part of block pulmonary resection. The early results are good enough to allow us to continue using the prosthesis in suitable cases.", "contents": "Tracheal reconstruction with a prosthesis of marlex mesh and pericardium. Tracheal reconstruction with a new type of prosthesis made of heavy Marlex mesh and pericardium is described in 13 patients suffering from malignancy. Two patients had circumferential excision of the trachea, and the remaining 11 had partial circumferential excision and a patch graft. In 10 of them, tracheal excision was a part of block pulmonary resection. The early results are good enough to allow us to continue using the prosthesis in suitable cases."}
{"id": "PMID:1090787", "title": "Blunt injuries to branches of the aortic arch.", "content": "Blunt injuries to branches of the aortic arch are not unusual and must be considered in any patient surviving deceleration or crush injury. Review of 36 case reports, including own own case, revealed 22 injuries to the innominate artery (4 with injuries to other arch branches), seven to the right subclavian, seven to the left subclavian, and eleven associated injuries to the thoracic aorta. Thirty patients (83 per cent) survived. Mediastinal widening (92 per cent) was the most frequent manifestation of vascular injury and is an indication for immediate aortography to delineate the entire thoracic aorta. Distal circulation was clinically decreased in less than 50 per cent, with symptomatic ischemia in only a few instances. Death was due to associated head injury in 3 of 6 cases. Earlier operation would have avoided exsanguination (one death) and late complications of false aneurysm or vascular insufficiency (10 patients).", "contents": "Blunt injuries to branches of the aortic arch. Blunt injuries to branches of the aortic arch are not unusual and must be considered in any patient surviving deceleration or crush injury. Review of 36 case reports, including own own case, revealed 22 injuries to the innominate artery (4 with injuries to other arch branches), seven to the right subclavian, seven to the left subclavian, and eleven associated injuries to the thoracic aorta. Thirty patients (83 per cent) survived. Mediastinal widening (92 per cent) was the most frequent manifestation of vascular injury and is an indication for immediate aortography to delineate the entire thoracic aorta. Distal circulation was clinically decreased in less than 50 per cent, with symptomatic ischemia in only a few instances. Death was due to associated head injury in 3 of 6 cases. Earlier operation would have avoided exsanguination (one death) and late complications of false aneurysm or vascular insufficiency (10 patients)."}
{"id": "PMID:1090789", "title": "Blood cultures: principles and techniques.", "content": "Detecting the presence of microorganisms in blood is an important function of the clinical microbiology laboratory. With prompt isolation, identification, and antimicrobial susceptibility testing of bacterial isolates, the clinician can modify the selection and dosage of the antimicrobics used initially to treat a suspected septicemia. Principles and techniques for blood collection, culture, examination, and reporting are reviewed.", "contents": "Blood cultures: principles and techniques. Detecting the presence of microorganisms in blood is an important function of the clinical microbiology laboratory. With prompt isolation, identification, and antimicrobial susceptibility testing of bacterial isolates, the clinician can modify the selection and dosage of the antimicrobics used initially to treat a suspected septicemia. Principles and techniques for blood collection, culture, examination, and reporting are reviewed."}
{"id": "PMID:1090790", "title": "Focal sclerosing glomerulonephropathy: a clinicopathologic study.", "content": "Forty cases of focal sclerosing glomerulonephropathy with nephrotic syndrome or proteinuria were studied retrospectively in regard to clinical presentation, response to steroid therapy and clinical course, and histopathology of the lesion.  Morphologically there was a focal segmental and global sclerosis with subendothelial hyaline deposits, collapse of the capillary loops, intracapillary hyaline material or foam cells, filling and widening of the mesangium with mesangial matrix, focal tubular atrophy, and focal interstitial fibrosis.  Thirty-four patients had been treated with prednisone; initial complete remission of the nephrotic syndrome occurred in only 4 patients and partial remission in 10.  Nine of these 14 patients had nephrotic relapse or became resistant to steroids.  Thirty-three percent of the patients progressed to end-stage renal failure and an additional 25 percent had impairment of renal function after a mean of 8 years from onset.  Three patients received kidney allografts, and in two the disease recurred in the transplanted kidney.  Focal sclerosing glomerulonephropathy associated with nephrotic syndrome or proteinuria appears to be a clinicopathologic entity characterized by resistance to steroid treatment, frequent progression to end-stage renal disease, and recurrence in the transplanted kidney.", "contents": "Focal sclerosing glomerulonephropathy: a clinicopathologic study. Forty cases of focal sclerosing glomerulonephropathy with nephrotic syndrome or proteinuria were studied retrospectively in regard to clinical presentation, response to steroid therapy and clinical course, and histopathology of the lesion.  Morphologically there was a focal segmental and global sclerosis with subendothelial hyaline deposits, collapse of the capillary loops, intracapillary hyaline material or foam cells, filling and widening of the mesangium with mesangial matrix, focal tubular atrophy, and focal interstitial fibrosis.  Thirty-four patients had been treated with prednisone; initial complete remission of the nephrotic syndrome occurred in only 4 patients and partial remission in 10.  Nine of these 14 patients had nephrotic relapse or became resistant to steroids.  Thirty-three percent of the patients progressed to end-stage renal failure and an additional 25 percent had impairment of renal function after a mean of 8 years from onset.  Three patients received kidney allografts, and in two the disease recurred in the transplanted kidney.  Focal sclerosing glomerulonephropathy associated with nephrotic syndrome or proteinuria appears to be a clinicopathologic entity characterized by resistance to steroid treatment, frequent progression to end-stage renal disease, and recurrence in the transplanted kidney."}
{"id": "PMID:1090791", "title": "Left ventricular function and myocardial contractility: analysis by roentgen videoangiographic techniques.", "content": "Conventional attempts to derive indices of myocardial contractility from left ventricular pump function appear to be unsound in practice and theory, and the small number of dimensional measuremets on which these computations are based obscure the regional differences in ventricular myocardial function.  It is not surprising that these indices have not proved to be fully satisfactory, either in research or in clinical laboratory settings.  Both theoretical considerations and laboratory results support the need for more detailed measurements of regional myocardial function.  These can be obtained by use of multiplanar videoroentgenographic techniques for cross-sectional reconstructions of the epicardial and endocardial surfaces of the heart throughout the cardiac cycle and the application of computer-based sophisticated methods of analysis, such as the finiteelement technique, as a basis for more accurate assessment of myocardial function.", "contents": "Left ventricular function and myocardial contractility: analysis by roentgen videoangiographic techniques. Conventional attempts to derive indices of myocardial contractility from left ventricular pump function appear to be unsound in practice and theory, and the small number of dimensional measuremets on which these computations are based obscure the regional differences in ventricular myocardial function.  It is not surprising that these indices have not proved to be fully satisfactory, either in research or in clinical laboratory settings.  Both theoretical considerations and laboratory results support the need for more detailed measurements of regional myocardial function.  These can be obtained by use of multiplanar videoroentgenographic techniques for cross-sectional reconstructions of the epicardial and endocardial surfaces of the heart throughout the cardiac cycle and the application of computer-based sophisticated methods of analysis, such as the finiteelement technique, as a basis for more accurate assessment of myocardial function."}
{"id": "PMID:1090795", "title": "The hypereosinophilic syndrome: analysis of fourteen cases with review of the literature.", "content": "Several closely related disease entities make up the idiopathic hypereosinophilic syndrome (HES). The syndrome is manifest by persistent and prolonged eosinophilia with organ damage. A group of 14 patients had hematologic, cardiac, and neurologic abnormalities attributable to this disease. Patient survival and response to chemotherapy was significantly better in this group than in previously reported patients. The etiology of HES remains unknown, as does the mechanism of tissue damage.", "contents": "The hypereosinophilic syndrome: analysis of fourteen cases with review of the literature. Several closely related disease entities make up the idiopathic hypereosinophilic syndrome (HES). The syndrome is manifest by persistent and prolonged eosinophilia with organ damage. A group of 14 patients had hematologic, cardiac, and neurologic abnormalities attributable to this disease. Patient survival and response to chemotherapy was significantly better in this group than in previously reported patients. The etiology of HES remains unknown, as does the mechanism of tissue damage."}
{"id": "PMID:1090796", "title": "Management of mycosis fungoides--current status and future prospects.", "content": "Current knowledge concerning the course of mycosis fungoides and recognized prognostic factors have been reviewed. Those factors with prognostic significance at the time of biopsy diagnosis include age and the clinical findings of skin tumors, ulceration or palpable lymphadenopathy. During the course of disease, the development of skin tumors, ulceration or palpable lymphadenopathy were each associated with a poor prognosis and median survival was only 12 months if all those clinical parameters were present. Patients who developed overt visceral mycosis fungoides rarely survived more than a few months. Lymphocytopenia and the presence of malignant lymphoma in biopsied lymph nodes were also poor prognostic findings. The various modalities of therapy for proven mycosis fungoides were reviewed. Topical therapy and external irradiation were generally of symptomatic benefit only, but two recent studies have shown that aggressive use of topical nitrogen mustard and electron beam therapy are associated with long-term responses in patients with disease confined to the skin. Single agent chemotherapy often resulted in transient responses in advanced and refractory mycosis fungoides. Future approaches to the management of mycosis fungoides have been suggested. These include a thorough review of the histological features, a thorough and systematic pretreatment evaluation and randomized studies of the various treatment modalities including combination therapy in appropriately staged patients.", "contents": "Management of mycosis fungoides--current status and future prospects. Current knowledge concerning the course of mycosis fungoides and recognized prognostic factors have been reviewed. Those factors with prognostic significance at the time of biopsy diagnosis include age and the clinical findings of skin tumors, ulceration or palpable lymphadenopathy. During the course of disease, the development of skin tumors, ulceration or palpable lymphadenopathy were each associated with a poor prognosis and median survival was only 12 months if all those clinical parameters were present. Patients who developed overt visceral mycosis fungoides rarely survived more than a few months. Lymphocytopenia and the presence of malignant lymphoma in biopsied lymph nodes were also poor prognostic findings. The various modalities of therapy for proven mycosis fungoides were reviewed. Topical therapy and external irradiation were generally of symptomatic benefit only, but two recent studies have shown that aggressive use of topical nitrogen mustard and electron beam therapy are associated with long-term responses in patients with disease confined to the skin. Single agent chemotherapy often resulted in transient responses in advanced and refractory mycosis fungoides. Future approaches to the management of mycosis fungoides have been suggested. These include a thorough review of the histological features, a thorough and systematic pretreatment evaluation and randomized studies of the various treatment modalities including combination therapy in appropriately staged patients."}
{"id": "PMID:1090805", "title": "Live attenuated versus killed virus vaccines.", "content": "The discussion of the problems of production and use of killed and live virus vaccines shows that the theoretical, basic concepts of the alternative vaccination procedures are equivalent in regard to the ideals of calculable potency and safety. If one compares the present stages of development, then it becomes evident that the advantages of live viral vaccination are due to greater potency. However, killed virus vaccines are safer. Nevertheless, these differences are not without limits. In some vaccinating procedures, the live virus vaccine is the safer of the two.", "contents": "Live attenuated versus killed virus vaccines. The discussion of the problems of production and use of killed and live virus vaccines shows that the theoretical, basic concepts of the alternative vaccination procedures are equivalent in regard to the ideals of calculable potency and safety. If one compares the present stages of development, then it becomes evident that the advantages of live viral vaccination are due to greater potency. However, killed virus vaccines are safer. Nevertheless, these differences are not without limits. In some vaccinating procedures, the live virus vaccine is the safer of the two."}
{"id": "PMID:1090809", "title": "[The influence of cardiogreen on the albumin binding of bilirubin in the neonatal period].", "content": "The albumin-binding of bilirubin may be disturbed by pharmacologically inert substances. This is true for Cardiogreen as used for dye dilution curves. The displacement of bilirubin from its albumin-bindung, examined in vitro by Sephadex filtration, is stronger for Cardiogreen than for Gantrisin known to cause kernicterus even in mild hyperbilirubinemia. However, from differences in dosage and the pharmacokinetics of the two substances it may be concluded, that the risk of possibly causing a kernicterus by Cardiogreen is limited to only extrem cases.", "contents": "[The influence of cardiogreen on the albumin binding of bilirubin in the neonatal period]. The albumin-binding of bilirubin may be disturbed by pharmacologically inert substances. This is true for Cardiogreen as used for dye dilution curves. The displacement of bilirubin from its albumin-bindung, examined in vitro by Sephadex filtration, is stronger for Cardiogreen than for Gantrisin known to cause kernicterus even in mild hyperbilirubinemia. However, from differences in dosage and the pharmacokinetics of the two substances it may be concluded, that the risk of possibly causing a kernicterus by Cardiogreen is limited to only extrem cases."}
{"id": "PMID:1090810", "title": "[Antibiotics in newborns and young infants].", "content": "Localized and generalized infections in newborns nowadays are mostly due to gram negative bacteria (E. coli, Klebsiella, Enterobacter, Pseudomonas pyocyanea, Serratia marcescens et al.). Unspecific treatment causes an increasing rate of resistance, as shown by an account of our own experiences. Tobramycin, Gentamycin, and Cefazolin appear to be particularly promising antibiotics to avoid the development of resistance though their pharmacokinetics in the newborn have to be considered carefully. Based on the determination of serum levels of Tobramycin and Amoxycillin in newborns general recommendations for the dosage of these new antibiotics are given.", "contents": "[Antibiotics in newborns and young infants]. Localized and generalized infections in newborns nowadays are mostly due to gram negative bacteria (E. coli, Klebsiella, Enterobacter, Pseudomonas pyocyanea, Serratia marcescens et al.). Unspecific treatment causes an increasing rate of resistance, as shown by an account of our own experiences. Tobramycin, Gentamycin, and Cefazolin appear to be particularly promising antibiotics to avoid the development of resistance though their pharmacokinetics in the newborn have to be considered carefully. Based on the determination of serum levels of Tobramycin and Amoxycillin in newborns general recommendations for the dosage of these new antibiotics are given."}
{"id": "PMID:1090811", "title": "Induction of base-pair substitution and frameshift mutations in wild-type and repair-deficient strains of Salmonella typhimurium by the photodynamic action of methylene blue.", "content": "Induction of back mutations to prototrophy by methylene blue (MB)-sensitized photodynamic (PD) treatment has been studied in wild-type and repair-deficient strains of Salmonella typhimurium carrying either the base-pair substitution mutation hisG46 or the frameshift mutation hisD30529 We found that reversion of the hisG46 mutation was increased in a strain carrying a uvrB deletion and decreased in a strain carrying a recA-type mutation. Reversion of the hisD3052 (frameshift) mutation, on the other hand, was decreased in both uvrB deletion and recA-type strains. The former results are consistent with the hypothesis that the majority of MB-sensitized PD-INDUCED BASE-PAIR SUBSTITUTION MUTATIONS ARIse by a mechanism similar to that currently believed to be involved in UV mutagenesis. The latter results suggest that PD-induced frameshift mutations may arise in some other way, and two possible mechanisms involving sequential action of the excision repair and recombinational repair pathways are considered.", "contents": "Induction of base-pair substitution and frameshift mutations in wild-type and repair-deficient strains of Salmonella typhimurium by the photodynamic action of methylene blue. Induction of back mutations to prototrophy by methylene blue (MB)-sensitized photodynamic (PD) treatment has been studied in wild-type and repair-deficient strains of Salmonella typhimurium carrying either the base-pair substitution mutation hisG46 or the frameshift mutation hisD30529 We found that reversion of the hisG46 mutation was increased in a strain carrying a uvrB deletion and decreased in a strain carrying a recA-type mutation. Reversion of the hisD3052 (frameshift) mutation, on the other hand, was decreased in both uvrB deletion and recA-type strains. The former results are consistent with the hypothesis that the majority of MB-sensitized PD-INDUCED BASE-PAIR SUBSTITUTION MUTATIONS ARIse by a mechanism similar to that currently believed to be involved in UV mutagenesis. The latter results suggest that PD-induced frameshift mutations may arise in some other way, and two possible mechanisms involving sequential action of the excision repair and recombinational repair pathways are considered."}
{"id": "PMID:1090812", "title": "Test of recessive lethals in the mouse.", "content": "The presence of spontaneous lethal mutations in inbred strains is discussed with special reference to their variation and influence on estimates of induced mutations. A model is presented that will facilitate classification of lethal-free and lethal heterozygotes. The model is used in classification of sons to lethal heterozygous males carrying a spontaneous mutation. The observed results are in good agreement with the model. From experience it is concluded that the most efficient way to use the facilities in lethal tests it to examine 10 or more full brothers to the P parents. By doing so pre-existing spontaneous lethals can be excluded by eliminating families in which any of the P parents were lethal heterozygous.  The observed total rate of recessive lethals gives slight over-estimation of the induced rate of mutations, as spontaneous mutations in the gametes forming the F1 cannot be excluded.", "contents": "Test of recessive lethals in the mouse. The presence of spontaneous lethal mutations in inbred strains is discussed with special reference to their variation and influence on estimates of induced mutations. A model is presented that will facilitate classification of lethal-free and lethal heterozygotes. The model is used in classification of sons to lethal heterozygous males carrying a spontaneous mutation. The observed results are in good agreement with the model. From experience it is concluded that the most efficient way to use the facilities in lethal tests it to examine 10 or more full brothers to the P parents. By doing so pre-existing spontaneous lethals can be excluded by eliminating families in which any of the P parents were lethal heterozygous.  The observed total rate of recessive lethals gives slight over-estimation of the induced rate of mutations, as spontaneous mutations in the gametes forming the F1 cannot be excluded."}
{"id": "PMID:1090813", "title": "Spontaneous recessive lethal mutations in the mouse.", "content": "The estimation of the load of recessive lethals in our inbred CBA strain is based on samples drawn from 1964 to 1971. 407 control F1 males were back-crossed to their daughters. The first examined females which for each F1  male gave 50 implants were included in the test. their rate of intra-uterine death was compared to that from lethal-free males. The distribution of the number of F1 males according to the number of dead in the first 50 implants were compared to a model of l\u00fcning. Three estimates of the proportion of lethal heterozygotes gave 4.2, 5.35 and 6.2% respectively. From the way the inbred strain was kept it was estimated that the mean load among F1 males was 11.5mu (mu equal to mutation rate per gamete). These data indicate that about 0.5% lethals occur per gamete with an upper limit of 1%. Comparisons are made with previous estimates of the spontaneous rate of lethal mutations per gamete. The low proportion of lethal mutations indicate that the number of loci giving lethal mutations is low. Both spontaneous and induced mutations give similar low numbers, more than one order of magnitude below the number commonly assumed. The uncertainties are stressed of estimating risk by multiplying mutation rate per locus and assumed number of loci.", "contents": "Spontaneous recessive lethal mutations in the mouse. The estimation of the load of recessive lethals in our inbred CBA strain is based on samples drawn from 1964 to 1971. 407 control F1 males were back-crossed to their daughters. The first examined females which for each F1  male gave 50 implants were included in the test. their rate of intra-uterine death was compared to that from lethal-free males. The distribution of the number of F1 males according to the number of dead in the first 50 implants were compared to a model of l\u00fcning. Three estimates of the proportion of lethal heterozygotes gave 4.2, 5.35 and 6.2% respectively. From the way the inbred strain was kept it was estimated that the mean load among F1 males was 11.5mu (mu equal to mutation rate per gamete). These data indicate that about 0.5% lethals occur per gamete with an upper limit of 1%. Comparisons are made with previous estimates of the spontaneous rate of lethal mutations per gamete. The low proportion of lethal mutations indicate that the number of loci giving lethal mutations is low. Both spontaneous and induced mutations give similar low numbers, more than one order of magnitude below the number commonly assumed. The uncertainties are stressed of estimating risk by multiplying mutation rate per locus and assumed number of loci."}
{"id": "PMID:1090815", "title": "Interactive mutagenicity of sodium nitrite, dimethylamine, methylurea and ethylurea.", "content": "Groups of mice were treated per os with sodium nitrite either alone or in combination with nitrosatable amino compounds and tested in the host mediated assay. When mice were treated with sodium nitrite in combination with dimethylamine a small(4-fold) but significant increase in mutant frequency (MF) was observed. Ethylurea or methylurea in combination with sodium nitrite induced 10- or 850-fold increases in MF, respectively. The response to methylurea was dose-dependent with a 6- and 30-fold increase in MF at 5.4 and 11.5 mg/kg NaNO2 and a 6-fold increase at 108 mg/kg methylurea. That this response reflected gastric nitrosation was shown by the disappearance of the response if NaNO2 administration preceded methylurea treatment by 10 min. High MF's were observed if NaNO2 was administered 10 or 20 min after methylurea.", "contents": "Interactive mutagenicity of sodium nitrite, dimethylamine, methylurea and ethylurea. Groups of mice were treated per os with sodium nitrite either alone or in combination with nitrosatable amino compounds and tested in the host mediated assay. When mice were treated with sodium nitrite in combination with dimethylamine a small(4-fold) but significant increase in mutant frequency (MF) was observed. Ethylurea or methylurea in combination with sodium nitrite induced 10- or 850-fold increases in MF, respectively. The response to methylurea was dose-dependent with a 6- and 30-fold increase in MF at 5.4 and 11.5 mg/kg NaNO2 and a 6-fold increase at 108 mg/kg methylurea. That this response reflected gastric nitrosation was shown by the disappearance of the response if NaNO2 administration preceded methylurea treatment by 10 min. High MF's were observed if NaNO2 was administered 10 or 20 min after methylurea."}
{"id": "PMID:1090818", "title": "Quantitative relationship between carcinogenicity and mutagenicity of polyaromatic hydrocarbons in Salmonella typhimurium mutants.", "content": "Mutagenic activities of various polyaromatic hydrocarbons (PAHs) in air pollutants, which are different in carcinogenic activities from each other, were examined with a set of four strains of Salmonella typhimurium (TAI535 series; deep rough strains without excision repair). All the compounds tested were converted to frameshift mutagens when they were metabolized by rat liver homogenate. There was a clear quantitative correlation between carcinogenicity and mutagenicity of PAHs tested in strain TAI538 using the rat liver enzyme induced with both dibenz(a,h)-anthracene and phenobarbital. On the other hand, such a correlation was not obvious in strain TAI537.", "contents": "Quantitative relationship between carcinogenicity and mutagenicity of polyaromatic hydrocarbons in Salmonella typhimurium mutants. Mutagenic activities of various polyaromatic hydrocarbons (PAHs) in air pollutants, which are different in carcinogenic activities from each other, were examined with a set of four strains of Salmonella typhimurium (TAI535 series; deep rough strains without excision repair). All the compounds tested were converted to frameshift mutagens when they were metabolized by rat liver homogenate. There was a clear quantitative correlation between carcinogenicity and mutagenicity of PAHs tested in strain TAI538 using the rat liver enzyme induced with both dibenz(a,h)-anthracene and phenobarbital. On the other hand, such a correlation was not obvious in strain TAI537."}
{"id": "PMID:1090826", "title": "Zinc therapy of acrodermatitis enteropathica.", "content": "The therapeutic effect of orally administered zinc was evaluated in an adult woman with acrodermatitis enteropathica. When she was off therapy and in clinical relapse the plasma zinc concentration (10 mug per 100 ml), serum alkaline phosphatase (3 1U per liter) and urine zinc excretion rate (39 mug per 24 hours) were extremely low. Di-iodohydroxyquin therapy was accompanied by a modest increase in plasma zinc concentrations. Oral zinc sulfate (220 mg three times a day or 50 mg twice a day) resulted in rapid and complete clinical remission, and in a return of plasma zinc, serum alkaline phosphatase and urinary zinc excretion to normal. These data are compatible with a severe zinc deficiency state and indicate that the inherited defect in this disease is either in or closely related to zinc metabolism. The beneficial effects of zinc therapy in this patient provide further confirmation of the efficacy of oral zinc in the treatment of acrodermatitis enteropathica.", "contents": "Zinc therapy of acrodermatitis enteropathica. The therapeutic effect of orally administered zinc was evaluated in an adult woman with acrodermatitis enteropathica. When she was off therapy and in clinical relapse the plasma zinc concentration (10 mug per 100 ml), serum alkaline phosphatase (3 1U per liter) and urine zinc excretion rate (39 mug per 24 hours) were extremely low. Di-iodohydroxyquin therapy was accompanied by a modest increase in plasma zinc concentrations. Oral zinc sulfate (220 mg three times a day or 50 mg twice a day) resulted in rapid and complete clinical remission, and in a return of plasma zinc, serum alkaline phosphatase and urinary zinc excretion to normal. These data are compatible with a severe zinc deficiency state and indicate that the inherited defect in this disease is either in or closely related to zinc metabolism. The beneficial effects of zinc therapy in this patient provide further confirmation of the efficacy of oral zinc in the treatment of acrodermatitis enteropathica."}
{"id": "PMID:1090841", "title": "Daily variations of FSH, LH and testosterone response to intravenous luteinizing hormone-releasing factor (LRF) in normal men.", "content": "The responses of FSH, LH and testosterone to acute stimulation with synthetic LRF were studied in 6 healthy, fertile men aged 33.4 plus or minus 1.6 yr (X plus or minus SE). Fifty mug of LRF were given, iv at 0600 h, 1200 h, 1800 h and 0000 h, at 1-week intervals, to all 6 volunteers simultaneously. Blood samples were collected by venipuncture before (-5 and 0 min) and 8, 16, 32, 64 and 128 min after LRF injection. Plasma levels of FSH, LH and testosterone were determined by double antibody radioimmunoassay techniques. The responses of FSH and LH to LRF injection showed a clear difference at the times studied. Maximal values were obtained at 0600 h and 1800 h while the response at noon was not significant for LH and absent for FSH. Testosterone secretion showed a clear-cut response to LRF in all the subjects. At three of the four studied times (0600 h, 1800 h, 000 h) plasma testosterone was already increased at 8 min reaching its maximum at 16 min and persisted high until the end of the study. The noon response reached its maximum at the end of the test period. The daily variations of FSH and LH responses to acute LRF stimulation should be taken into consideration in clinical practive and the increment in testosterone secretion makes this test a useful indicator for androgenic testicular reserve.", "contents": "Daily variations of FSH, LH and testosterone response to intravenous luteinizing hormone-releasing factor (LRF) in normal men. The responses of FSH, LH and testosterone to acute stimulation with synthetic LRF were studied in 6 healthy, fertile men aged 33.4 plus or minus 1.6 yr (X plus or minus SE). Fifty mug of LRF were given, iv at 0600 h, 1200 h, 1800 h and 0000 h, at 1-week intervals, to all 6 volunteers simultaneously. Blood samples were collected by venipuncture before (-5 and 0 min) and 8, 16, 32, 64 and 128 min after LRF injection. Plasma levels of FSH, LH and testosterone were determined by double antibody radioimmunoassay techniques. The responses of FSH and LH to LRF injection showed a clear difference at the times studied. Maximal values were obtained at 0600 h and 1800 h while the response at noon was not significant for LH and absent for FSH. Testosterone secretion showed a clear-cut response to LRF in all the subjects. At three of the four studied times (0600 h, 1800 h, 000 h) plasma testosterone was already increased at 8 min reaching its maximum at 16 min and persisted high until the end of the study. The noon response reached its maximum at the end of the test period. The daily variations of FSH and LH responses to acute LRF stimulation should be taken into consideration in clinical practive and the increment in testosterone secretion makes this test a useful indicator for androgenic testicular reserve."}
{"id": "PMID:1090842", "title": "The response of pituitary gonadotropes to synthetic LRF in children with glucocorticoid-treated congenital adrenal hyperplasia: lack of effect of intrauterine and neonatal androgen excess.", "content": "Previous studies have demonstrated that the secretory reserve of the pituitary gland during childhood and adolescence is characterized by an age-related increase in LH secretion and a sex dichotomy of FSH secretion evoked by LRF. To determine if prenatal and neonatal androgen excess alters hypothalamic-pituitary-gonadal function, as in the neonatally androgenized rodent, we assessed pituitary sensitivity to 100 mug synthetic LRF in 9 girls and 2 boys with glucorticoid-treated congenital virilizing adrenal hyperplasia (CAH). In the 7 prepubertal girls with CAH, plasma LH following LRF rose to 2.0 plus or minus 0.4 (SE) ng/ml (LER 960) and did not differ from normal prepubertal children (1.7 plus or minus 0.1) and was lower (P less than 0.002) than in normal pubertal children (4.9 plus or minus 0.3). The 2 pubertal girls had LH rises in the pubertal range. In the 2 boys with CAH, the one with the more advanced bone age, 11-6/12 yr, developed true precocious puberty following initiation of cortisone treatment and had a pubertal LRF-evoked LH release on 3 occasions; the more immature boy, bone age 10, had a prepubertal LH rise. In the 7 prepubertal girls with CAH, plasma FSH rose to 8.1 plus or minus 1.6 (SE) ng/ml (LER 869), which did not differ from normal prepubertal girls (5.3 plus or minus 1.9), but was significantly (P less than 0.001) greater than in normal prepubertal boys (2.9 plus or minus 0.4). In these girls with CAH, basal plasma T (27.4 plus or minus 4.0 (SE) ng/dl) and 17-OHP (2703 plus or minus 1143 (SE) ng/dl) were greater than in prepubertal children, but plasma E1 and E2 were in the normal range. The pubertal boy had plasma T, E1, and E2 levels appropriate for his degree of sexual maturation. The results suggest: (1) age-related LH secretion evoked by LRF in glucocorticoid-treated CAH is appropriate for skeletal maturation; (2) the sex dichotomy of FSH secretion following LRF is preserved in CAH despite intrauterine and variable postnatal exposure to excess androgen and continuing slightly elevated plasma T during childhood; and (3) precocious puberty may follow initiation of cortisone treatment in a child with CAH whose bone age and secretory reserve of pituitary gonadotropins are in the pubertal range.", "contents": "The response of pituitary gonadotropes to synthetic LRF in children with glucocorticoid-treated congenital adrenal hyperplasia: lack of effect of intrauterine and neonatal androgen excess. Previous studies have demonstrated that the secretory reserve of the pituitary gland during childhood and adolescence is characterized by an age-related increase in LH secretion and a sex dichotomy of FSH secretion evoked by LRF. To determine if prenatal and neonatal androgen excess alters hypothalamic-pituitary-gonadal function, as in the neonatally androgenized rodent, we assessed pituitary sensitivity to 100 mug synthetic LRF in 9 girls and 2 boys with glucorticoid-treated congenital virilizing adrenal hyperplasia (CAH). In the 7 prepubertal girls with CAH, plasma LH following LRF rose to 2.0 plus or minus 0.4 (SE) ng/ml (LER 960) and did not differ from normal prepubertal children (1.7 plus or minus 0.1) and was lower (P less than 0.002) than in normal pubertal children (4.9 plus or minus 0.3). The 2 pubertal girls had LH rises in the pubertal range. In the 2 boys with CAH, the one with the more advanced bone age, 11-6/12 yr, developed true precocious puberty following initiation of cortisone treatment and had a pubertal LRF-evoked LH release on 3 occasions; the more immature boy, bone age 10, had a prepubertal LH rise. In the 7 prepubertal girls with CAH, plasma FSH rose to 8.1 plus or minus 1.6 (SE) ng/ml (LER 869), which did not differ from normal prepubertal girls (5.3 plus or minus 1.9), but was significantly (P less than 0.001) greater than in normal prepubertal boys (2.9 plus or minus 0.4). In these girls with CAH, basal plasma T (27.4 plus or minus 4.0 (SE) ng/dl) and 17-OHP (2703 plus or minus 1143 (SE) ng/dl) were greater than in prepubertal children, but plasma E1 and E2 were in the normal range. The pubertal boy had plasma T, E1, and E2 levels appropriate for his degree of sexual maturation. The results suggest: (1) age-related LH secretion evoked by LRF in glucocorticoid-treated CAH is appropriate for skeletal maturation; (2) the sex dichotomy of FSH secretion following LRF is preserved in CAH despite intrauterine and variable postnatal exposure to excess androgen and continuing slightly elevated plasma T during childhood; and (3) precocious puberty may follow initiation of cortisone treatment in a child with CAH whose bone age and secretory reserve of pituitary gonadotropins are in the pubertal range."}
{"id": "PMID:1090851", "title": "Bacterial behaviour.", "content": "Bacteria swim by rotating their flagella. They back up or choose new directions at random by changing the direction of the rotation. The probability of such changes is biased by sensory reception. The bias depends on the way in which the intensity ofthe stimulus changes with time, so that the bacteria tend to swim up a gradient of attractant and down a gradient of repellent chemicals.", "contents": "Bacterial behaviour. Bacteria swim by rotating their flagella. They back up or choose new directions at random by changing the direction of the rotation. The probability of such changes is biased by sensory reception. The bias depends on the way in which the intensity ofthe stimulus changes with time, so that the bacteria tend to swim up a gradient of attractant and down a gradient of repellent chemicals."}
{"id": "PMID:1090853", "title": "Chemical structure and biological activity of endotoxins (lipopolysaccharides) and lipid A.", "content": "Lipopolysaccharides (endotoxins) of gram-negative bacteria consist of two components with distinct physico-chemical character: a heteropolysaccharide and a covalently linked lipid, termed lipid A.  Chemically, lipid A is made up of acylated glucosamine disaccharides, which are interlinked by pyrophosphate bridges.  Lipid A represents the toxic center of lipopolysaccharides.  In rabbits, lipid A also induces pyrogen tolerance as well as pyrogen cross-tolerance.  Fever tolerance can be passively transferred with serum from rabbits immunized with lipid A.  The protective power of lipid A antiserum, however, is only expressed in amimals which have been pretreated with lipid A or lipopolysaccharide, indicating that other than humoral factors, perhaps cellular, also participate in endotoxin tolerance.  Lipid A antiserum also prevents the local Shwartzman reaction in rabbits.  The possible potency of lipid A antiserum to prevent other endotoxin effects such as lethal shock is presently investigated.", "contents": "Chemical structure and biological activity of endotoxins (lipopolysaccharides) and lipid A. Lipopolysaccharides (endotoxins) of gram-negative bacteria consist of two components with distinct physico-chemical character: a heteropolysaccharide and a covalently linked lipid, termed lipid A.  Chemically, lipid A is made up of acylated glucosamine disaccharides, which are interlinked by pyrophosphate bridges.  Lipid A represents the toxic center of lipopolysaccharides.  In rabbits, lipid A also induces pyrogen tolerance as well as pyrogen cross-tolerance.  Fever tolerance can be passively transferred with serum from rabbits immunized with lipid A.  The protective power of lipid A antiserum, however, is only expressed in amimals which have been pretreated with lipid A or lipopolysaccharide, indicating that other than humoral factors, perhaps cellular, also participate in endotoxin tolerance.  Lipid A antiserum also prevents the local Shwartzman reaction in rabbits.  The possible potency of lipid A antiserum to prevent other endotoxin effects such as lethal shock is presently investigated."}
{"id": "PMID:1090860", "title": "[Prevention and treatment of edema due to arterial revascularization].", "content": "Mean daily doses of 4-800,000 units iant. Cal. Trasylol administered i.v. for about 7 days prevented oedema and improved circulation after revascularisation surgery of the lower extremities involving the femoropopliteal axis in a large number of clinical cases.", "contents": "[Prevention and treatment of edema due to arterial revascularization]. Mean daily doses of 4-800,000 units iant. Cal. Trasylol administered i.v. for about 7 days prevented oedema and improved circulation after revascularisation surgery of the lower extremities involving the femoropopliteal axis in a large number of clinical cases."}
{"id": "PMID:1090863", "title": "Pelvic exenteration for vaginal embryonal rhabdomyosarcoma: a review.", "content": "The English language literature for the years 1935-1972 was reviewed to assess the effect of pelvic exenteration on vaginal embryonal rhabdomyosarcoma. Twenty-one cases were determined eligible for review. Tumor was confined to the vagina in 8 cases and extended beyond the vagina in 13 cases. Treatment by pelvic exenteration was found to be relatively ineffective means of curing patients with tumor extending beyond the limits of the vagina. Recent evidence from the literature indicates that better results can be obtained by coordinated treatment, ie, surgery, radiation therapy, and/or chemotherapy, than by surgery alone.", "contents": "Pelvic exenteration for vaginal embryonal rhabdomyosarcoma: a review. The English language literature for the years 1935-1972 was reviewed to assess the effect of pelvic exenteration on vaginal embryonal rhabdomyosarcoma. Twenty-one cases were determined eligible for review. Tumor was confined to the vagina in 8 cases and extended beyond the vagina in 13 cases. Treatment by pelvic exenteration was found to be relatively ineffective means of curing patients with tumor extending beyond the limits of the vagina. Recent evidence from the literature indicates that better results can be obtained by coordinated treatment, ie, surgery, radiation therapy, and/or chemotherapy, than by surgery alone."}
{"id": "PMID:1090869", "title": "Long-range effects of Ivalon sponge containing isobutyl cyanoacrylates on rat tissue. A quantitative planimetric study.", "content": "This study on seventy rats was undertaken to determine the long-range effects of Ivalon sponge containing isobutyl cyanoacrylates (IBC). Histologic examination revealed that IBC sponge was well tolerated by the connective tissue. IBC sponge was most effective during the first 2 weeks of healing. At observation periods of 1 and 2 weeks, the percentage values for connective tissue formed in IBC-treated animals were 2.02 and 1.58 times higher than those in the untreated animals.", "contents": "Long-range effects of Ivalon sponge containing isobutyl cyanoacrylates on rat tissue. A quantitative planimetric study. This study on seventy rats was undertaken to determine the long-range effects of Ivalon sponge containing isobutyl cyanoacrylates (IBC). Histologic examination revealed that IBC sponge was well tolerated by the connective tissue. IBC sponge was most effective during the first 2 weeks of healing. At observation periods of 1 and 2 weeks, the percentage values for connective tissue formed in IBC-treated animals were 2.02 and 1.58 times higher than those in the untreated animals."}
{"id": "PMID:1090870", "title": "A rare and complex case of multifocal mucocutaneous lupus tuberculosis with isolated lesion of the tongue.", "content": "We have described a case of plurifocal tuberculous lupus with a localization limited to the tongue. This finding is remarkable because of its rarity. The weakly positive tuberculin reaction and the discovery of a concomitant myeloproliferative syndrome add interest and complexity to the case.", "contents": "A rare and complex case of multifocal mucocutaneous lupus tuberculosis with isolated lesion of the tongue. We have described a case of plurifocal tuberculous lupus with a localization limited to the tongue. This finding is remarkable because of its rarity. The weakly positive tuberculin reaction and the discovery of a concomitant myeloproliferative syndrome add interest and complexity to the case."}
{"id": "PMID:1090871", "title": "Evaluation of root canal preparation with two automated endodontic handpieces.", "content": "An evaluation of automated low-speed handpiece preparation of canals was made by a silicone injection technique. These preparations were compared and rated with canals prepared by conventional hand instrumentation. Canals were graded on the criteria of shape, smoothness, elimation of morphologic aberrations, and apical preparations. Conventional hand istrumentation proved to be superior and required approximately the same amount of time as automated ins-rumentation in this vitro study.", "contents": "Evaluation of root canal preparation with two automated endodontic handpieces. An evaluation of automated low-speed handpiece preparation of canals was made by a silicone injection technique. These preparations were compared and rated with canals prepared by conventional hand instrumentation. Canals were graded on the criteria of shape, smoothness, elimation of morphologic aberrations, and apical preparations. Conventional hand istrumentation proved to be superior and required approximately the same amount of time as automated ins-rumentation in this vitro study."}
{"id": "PMID:1090872", "title": "Nonspecific stomatitis-a presenting sign in pernicious anemia.", "content": "Recurrent nonspecific stomatitis may be an oral sign of pernicious anemia. Repeated examinations and blood studies are important when the cause of stomatitis is not clear on initial evaluation of a complaint of sore mouth. Three cases of pernicious anemia are presented to illustrate the similarities and differences in oral signs of pernicious anemia.", "contents": "Nonspecific stomatitis-a presenting sign in pernicious anemia. Recurrent nonspecific stomatitis may be an oral sign of pernicious anemia. Repeated examinations and blood studies are important when the cause of stomatitis is not clear on initial evaluation of a complaint of sore mouth. Three cases of pernicious anemia are presented to illustrate the similarities and differences in oral signs of pernicious anemia."}
{"id": "PMID:1090885", "title": "Symposium on sensorineural hearing loss in children: early detection and intervention. Delivery systems in the management of hearing impaired children.", "content": "The management of a hearing impaired child involves the family as well as the child himself, especially when the child is very young. Delivery systems therefore must include the parents as an integral part of the delivery chain and be sensitive to the parents' need to understand the treatment or therapy and, when appropriate, to sustain it in the home. Health care services that have educational implications have been termed eduhealth, which in the case of the hearing impaired child, refers to such services as the early detection of hearing loss, the proper fitting of hearing aids, the prompt treatment of upper respiratory infections that might lead to further hearing loss, and vision screening. The otolaryngologist, audiologist, and parent all have eduhealth responsibilities. Professionals in medicine and allied fields, although well trained and experienced in their specialities, are rarely prepared to meet all the needs of the young hearing impaired child and his parents with appropriate services and in the manner most appropriate for them. Some agencies, however, have demonstrated comprehensive capabilities in this area. In referring a family to a delivery system, the otolaryngologist should establish to his own satisfaction that the requisite skills are present. Common complaints of parents of hearing impaired children are the lack of communication with the physician and clinician, which is frequently the result of the vocabulary used by the specialist and the limited time spent with the parents during visits. The result can be parental failure to follow instructions and possibly irreversible damage to the child's development of listening, language, and speech skills. The eduhealth delivery service index is a new attempt to measure the effectiveness of systems for the delivery of education and health care services to young hearing impaired children and their parents. It has been demonstrated in a limited population and may prove useful in the comparison of delivery systems. The otolaryngologist is urged to become better acquainted with the various elements of delivery systems and to be cautious in accepting claims of comprehensive quality services until verified by his own experience or the reports of actual consumers of the services offered.", "contents": "Symposium on sensorineural hearing loss in children: early detection and intervention. Delivery systems in the management of hearing impaired children. The management of a hearing impaired child involves the family as well as the child himself, especially when the child is very young. Delivery systems therefore must include the parents as an integral part of the delivery chain and be sensitive to the parents' need to understand the treatment or therapy and, when appropriate, to sustain it in the home. Health care services that have educational implications have been termed eduhealth, which in the case of the hearing impaired child, refers to such services as the early detection of hearing loss, the proper fitting of hearing aids, the prompt treatment of upper respiratory infections that might lead to further hearing loss, and vision screening. The otolaryngologist, audiologist, and parent all have eduhealth responsibilities. Professionals in medicine and allied fields, although well trained and experienced in their specialities, are rarely prepared to meet all the needs of the young hearing impaired child and his parents with appropriate services and in the manner most appropriate for them. Some agencies, however, have demonstrated comprehensive capabilities in this area. In referring a family to a delivery system, the otolaryngologist should establish to his own satisfaction that the requisite skills are present. Common complaints of parents of hearing impaired children are the lack of communication with the physician and clinician, which is frequently the result of the vocabulary used by the specialist and the limited time spent with the parents during visits. The result can be parental failure to follow instructions and possibly irreversible damage to the child's development of listening, language, and speech skills. The eduhealth delivery service index is a new attempt to measure the effectiveness of systems for the delivery of education and health care services to young hearing impaired children and their parents. It has been demonstrated in a limited population and may prove useful in the comparison of delivery systems. The otolaryngologist is urged to become better acquainted with the various elements of delivery systems and to be cautious in accepting claims of comprehensive quality services until verified by his own experience or the reports of actual consumers of the services offered."}
{"id": "PMID:1090886", "title": "Symposium on sensorineural hearing loss in children: early detection and intervention. Genetic factors in deafness of early life.", "content": "One half of all cases of childhood deafness are genetically caused, and this proportion will become increasingly larger as other causes come under control. The mode of transmission of hereditary hearing loss may be dominant, recessive, or X linked. Many varieties of childhood deafness can be distinguished by their accompanying anomalies. Other types of hereditary deafness occur, without associated abnormalities. Certain of these types may be delineated by the mode of transmission, age of onset, stability or progression, and audiometric findings. Further research on delineation of nonsyndromic hearing loss in necessary if treatment of specific kinds is to become a reality. Research also is needed to detect the carrier state in recessive and X linked types of hearing loss. In the absence of means of prevention and treatment for most cases of hereditary deafness, genetic counseling remains the most valuable course.", "contents": "Symposium on sensorineural hearing loss in children: early detection and intervention. Genetic factors in deafness of early life. One half of all cases of childhood deafness are genetically caused, and this proportion will become increasingly larger as other causes come under control. The mode of transmission of hereditary hearing loss may be dominant, recessive, or X linked. Many varieties of childhood deafness can be distinguished by their accompanying anomalies. Other types of hereditary deafness occur, without associated abnormalities. Certain of these types may be delineated by the mode of transmission, age of onset, stability or progression, and audiometric findings. Further research on delineation of nonsyndromic hearing loss in necessary if treatment of specific kinds is to become a reality. Research also is needed to detect the carrier state in recessive and X linked types of hearing loss. In the absence of means of prevention and treatment for most cases of hereditary deafness, genetic counseling remains the most valuable course."}
{"id": "PMID:1090888", "title": "Symposium on sensorineural hearing loss in children: early detection and intervention. Classifications of auditory impairment.", "content": "Over the past 25 years there has been a consistently growing interest in the identification of hearing disorders in children. Basic to the identification of hearing loss are the degree of hearing loss, the age of onset, the etiology, and the site of the lesion. In this modern era, hearing loss should not go undetected, and a child with a hearing loss no longer has to wait until he is six years of age before he can be treated and educated. Very young children can be successfully tested for auditory problems, and every effort should be taken to detect and identify such children with hearing problems. The audiometric tests devised for adults have been adapted, in part, for the measurement of hearing in children. The threshold values derived from standard audiometry and play audiometry are equivalent to those in adults. If some consistency is used in classifying children with auditory impairment, future research will be enhanced in the areas of prevention and social and educational management of children with auditory impairment.", "contents": "Symposium on sensorineural hearing loss in children: early detection and intervention. Classifications of auditory impairment. Over the past 25 years there has been a consistently growing interest in the identification of hearing disorders in children. Basic to the identification of hearing loss are the degree of hearing loss, the age of onset, the etiology, and the site of the lesion. In this modern era, hearing loss should not go undetected, and a child with a hearing loss no longer has to wait until he is six years of age before he can be treated and educated. Very young children can be successfully tested for auditory problems, and every effort should be taken to detect and identify such children with hearing problems. The audiometric tests devised for adults have been adapted, in part, for the measurement of hearing in children. The threshold values derived from standard audiometry and play audiometry are equivalent to those in adults. If some consistency is used in classifying children with auditory impairment, future research will be enhanced in the areas of prevention and social and educational management of children with auditory impairment."}
{"id": "PMID:1090891", "title": "Responsivity of pituitary gonadotropes to luteinizing hormone-releasing factor in idiopathic precocious puberty, precocious thelarche, precocious adrenarche, and in patients treated with medroxyprogesterone acetate.", "content": "One hundred micrograms synthetic luteinizing hormone-releasing factor (LRF) were administered to 13 girls and 2 boys with idiopathic precocious puberty, 3 girls with precocious thelarche, 2 girls with precocious adrenarche, and 5 children treated with medroxyprogesterone acetate (MPA). Luteinizing hormone (LH), follicle-stimulating hormone (FSH), and sex steroid responses were assessed. The mean readily releasable LH rose to a peak of 8.4 plus or minus 1. 8 ng/ml (LER 960) in the children with idiopathic precocious puberty and was significantly greater than in normal prepubertal (1.8 plus or minus 0.14) or pubertal children (4.9 ng/ml) (LER 869) was higher in precocious puberty but not significantly greater than in normal prepubertal (5.3 plus or minus 1.9) or pubertal girls (6.0 plus or minus 1.2). The mean concentration of plasma estradiol rose significantly above resting levels after LRF in the girls with idiopathic precocious puberty. The LH response in girls with precocious the larche was in the prepubertal range. In 4 of 5 children with sexual precocity treated with MPA, the LH release evoked by LRF was diminished.", "contents": "Responsivity of pituitary gonadotropes to luteinizing hormone-releasing factor in idiopathic precocious puberty, precocious thelarche, precocious adrenarche, and in patients treated with medroxyprogesterone acetate. One hundred micrograms synthetic luteinizing hormone-releasing factor (LRF) were administered to 13 girls and 2 boys with idiopathic precocious puberty, 3 girls with precocious thelarche, 2 girls with precocious adrenarche, and 5 children treated with medroxyprogesterone acetate (MPA). Luteinizing hormone (LH), follicle-stimulating hormone (FSH), and sex steroid responses were assessed. The mean readily releasable LH rose to a peak of 8.4 plus or minus 1. 8 ng/ml (LER 960) in the children with idiopathic precocious puberty and was significantly greater than in normal prepubertal (1.8 plus or minus 0.14) or pubertal children (4.9 ng/ml) (LER 869) was higher in precocious puberty but not significantly greater than in normal prepubertal (5.3 plus or minus 1.9) or pubertal girls (6.0 plus or minus 1.2). The mean concentration of plasma estradiol rose significantly above resting levels after LRF in the girls with idiopathic precocious puberty. The LH response in girls with precocious the larche was in the prepubertal range. In 4 of 5 children with sexual precocity treated with MPA, the LH release evoked by LRF was diminished."}
{"id": "PMID:1090892", "title": "Pharmacologic manipulation of human erythrocyte 2,3-diphosphoglycerate levels by prednisone administration.", "content": "Erythrocyte 2,3-diphosphoglycerate (2,3-DPG) concentrations in 10 patients with acute lymphoblastic leukemia rose 21.3%(P smaller than 0.02) after 3 weeks of prednisone and vincristine therapy, and returned to pretreatment level 2 weeks after therapy had been discontinued. The mean 2,3-DPG level of three patients on vincristine alone did not vary significantly from the control level of the leukemia patients on prednisone and vincristine. No significant change in serum inorganic phosphate level was observed. The mean erythrocyte 2,3-DPG concentration of 17 nephrotic syndrome patients being treated with chronic prednisone therapy was 14.0% higher than a control group of 20 nephrotic syndrome patients not being treated with prednisone (P small than 0.01). A significant positive correlation was observed between the dose of prednisone and 2,3-DPG level.", "contents": "Pharmacologic manipulation of human erythrocyte 2,3-diphosphoglycerate levels by prednisone administration. Erythrocyte 2,3-diphosphoglycerate (2,3-DPG) concentrations in 10 patients with acute lymphoblastic leukemia rose 21.3%(P smaller than 0.02) after 3 weeks of prednisone and vincristine therapy, and returned to pretreatment level 2 weeks after therapy had been discontinued. The mean 2,3-DPG level of three patients on vincristine alone did not vary significantly from the control level of the leukemia patients on prednisone and vincristine. No significant change in serum inorganic phosphate level was observed. The mean erythrocyte 2,3-DPG concentration of 17 nephrotic syndrome patients being treated with chronic prednisone therapy was 14.0% higher than a control group of 20 nephrotic syndrome patients not being treated with prednisone (P small than 0.01). A significant positive correlation was observed between the dose of prednisone and 2,3-DPG level."}
{"id": "PMID:1090893", "title": "T and B lymphocyte subpopulations in children.", "content": "T and B lymphocyte subpopulations were examined in the peripheral blood of children using the E and EAC rosette assays. Children under 18 months of age were found to have a decreased percentage of E-binding (T) lymphocytes and an increased percentage of EAC-binding (B) lymphocytes compared to older children (18 months to 10 years) and adults. The absolute number of E-binding and EAC-binding lymphocytes was increased in children under 18 months of age.", "contents": "T and B lymphocyte subpopulations in children. T and B lymphocyte subpopulations were examined in the peripheral blood of children using the E and EAC rosette assays. Children under 18 months of age were found to have a decreased percentage of E-binding (T) lymphocytes and an increased percentage of EAC-binding (B) lymphocytes compared to older children (18 months to 10 years) and adults. The absolute number of E-binding and EAC-binding lymphocytes was increased in children under 18 months of age."}
{"id": "PMID:1090895", "title": "Transcutaneous oxygen tension measurements in newborn infants.", "content": "Attention is called in this brief review article to the development and clinical uses of a new technique for monitoring blood oxygen tensions in newborn infants. This technique uses an electrode recently developed by Huch et al. in Germany to measure arterial oxygen tensions directly through the skin. This device allows for continuous monitoring with a non-invasive technique. The method is now in use in European hospitals. Clinical experiences are cited to document its usefulness.", "contents": "Transcutaneous oxygen tension measurements in newborn infants. Attention is called in this brief review article to the development and clinical uses of a new technique for monitoring blood oxygen tensions in newborn infants. This technique uses an electrode recently developed by Huch et al. in Germany to measure arterial oxygen tensions directly through the skin. This device allows for continuous monitoring with a non-invasive technique. The method is now in use in European hospitals. Clinical experiences are cited to document its usefulness."}
{"id": "PMID:1090896", "title": "Suprarenal abscess in the neonate: a case report and review of diagnosis and management.", "content": "A case of unilateral suprarenal abscess is reported. This is the third such reported case, and the first reported case successfully treated with preservation of the kidney. Diagnosis and treatment were aided significantly by a thorough preoperative radiological evaluation, including angiography.", "contents": "Suprarenal abscess in the neonate: a case report and review of diagnosis and management. A case of unilateral suprarenal abscess is reported. This is the third such reported case, and the first reported case successfully treated with preservation of the kidney. Diagnosis and treatment were aided significantly by a thorough preoperative radiological evaluation, including angiography."}
{"id": "PMID:1090897", "title": "The use of indwelling radial artery catheters in neonates.", "content": "Indwelling radial artery catheterization was performed on 20 infants. In only one patient was it unsuccessful. Mean duration of indwelling catheterization was 44.1 hours. Advantages of this procedure include constant availability of arterial blood gas sampling and blood pressure monitoring. It is a relatively safe procedure offering a low incidence of clinical infection and serious vascular complications. Further experience will delineate its exact role in the care of the acutely ill neonate.", "contents": "The use of indwelling radial artery catheters in neonates. Indwelling radial artery catheterization was performed on 20 infants. In only one patient was it unsuccessful. Mean duration of indwelling catheterization was 44.1 hours. Advantages of this procedure include constant availability of arterial blood gas sampling and blood pressure monitoring. It is a relatively safe procedure offering a low incidence of clinical infection and serious vascular complications. Further experience will delineate its exact role in the care of the acutely ill neonate."}
{"id": "PMID:1090905", "title": "The efficacy of group therapy by nurses for hospitalized patients.", "content": "To investigate the effect of group therapy by nurses on chronic psychiatric patients, 100 chronic psychiatric patients between the ages of 30 and 59, hospitalized on two regressed, continued care wards and one community care preparation ward at the Veterans Administration Hospital, Augusta, Georgia, were paired, using the variables of age, diagnosis, and number and duration of hospitalizations, and assigned to five control and five experimental groups (ten patients per group). Experimental subjects attended group therapy sessions, led by two nurses, twice a week; control subjects did not. All subjects were evaluated by two nursing assistants on the NOSIE-30 scale prior to group therapy, after ten sessions, and at the end of 20 sessions. Paired t-tests were computed on pre- and postdata for six factors. Data from an evaluation scale of the most regressed ward indicated leader-patient interaction. Sociogram evaluation revealed 79 percent positive interactions. Topics most frequently mentioned were nature in regard to delusional systems and interpersonal relations in the hospital and home. Findings supported the use of group therapy with chronic regressed psychiatric patients.", "contents": "The efficacy of group therapy by nurses for hospitalized patients. To investigate the effect of group therapy by nurses on chronic psychiatric patients, 100 chronic psychiatric patients between the ages of 30 and 59, hospitalized on two regressed, continued care wards and one community care preparation ward at the Veterans Administration Hospital, Augusta, Georgia, were paired, using the variables of age, diagnosis, and number and duration of hospitalizations, and assigned to five control and five experimental groups (ten patients per group). Experimental subjects attended group therapy sessions, led by two nurses, twice a week; control subjects did not. All subjects were evaluated by two nursing assistants on the NOSIE-30 scale prior to group therapy, after ten sessions, and at the end of 20 sessions. Paired t-tests were computed on pre- and postdata for six factors. Data from an evaluation scale of the most regressed ward indicated leader-patient interaction. Sociogram evaluation revealed 79 percent positive interactions. Topics most frequently mentioned were nature in regard to delusional systems and interpersonal relations in the hospital and home. Findings supported the use of group therapy with chronic regressed psychiatric patients."}
{"id": "PMID:1090929", "title": "T4-induced RNA ligase joins single-stranded oligoribonucleotides.", "content": "RNA ligase isolated from Escherichia coli infected with bacteriophage T4 will catalyze the formation of an intermolecular 3' leads to 5' phosphodiester linkage between an oligoribonucleotide with a free 3'-hydroxyl and another oligoribonucleotide with a 5'-phosphate. Upon reaction with (Ap)5C, nearly quantitative conversion of the hexamer [5'-32P]p(Up)5U to the dodecamer (Ap)5C[3' leads to 5'-32P]p(Up)5U was observed. The product was identified by its mobility on RPC-5 column chromatography, its resistance to alkaline phosphatase, and the appearance of the expected radiolabeled products on hydrolysis with alkali, ribonuclease A, snake venom phosphodiesterase, and spleen phosphodiesterase. The coupling of other pairs of single-stranded oligoribonucleotides has also been demonstrated. The intermolecular joining reaction is probably mechanistically similar to the intramolecular cyclization activity previously reported for Tr RNA ligase. It is expected that this enzyme will be useful for the synthesis of RNA fragments of defined sequence.", "contents": "T4-induced RNA ligase joins single-stranded oligoribonucleotides. RNA ligase isolated from Escherichia coli infected with bacteriophage T4 will catalyze the formation of an intermolecular 3' leads to 5' phosphodiester linkage between an oligoribonucleotide with a free 3'-hydroxyl and another oligoribonucleotide with a 5'-phosphate. Upon reaction with (Ap)5C, nearly quantitative conversion of the hexamer [5'-32P]p(Up)5U to the dodecamer (Ap)5C[3' leads to 5'-32P]p(Up)5U was observed. The product was identified by its mobility on RPC-5 column chromatography, its resistance to alkaline phosphatase, and the appearance of the expected radiolabeled products on hydrolysis with alkali, ribonuclease A, snake venom phosphodiesterase, and spleen phosphodiesterase. The coupling of other pairs of single-stranded oligoribonucleotides has also been demonstrated. The intermolecular joining reaction is probably mechanistically similar to the intramolecular cyclization activity previously reported for Tr RNA ligase. It is expected that this enzyme will be useful for the synthesis of RNA fragments of defined sequence."}
{"id": "PMID:1090930", "title": "Microbial production of ammonium ion from nitrogen.", "content": "Genetic manipulation of nitrogenase and key glutamate-forming enzymes can provide mutants that excrete fixed N2 as NH4+. A derepressed N2 fxation mutant (SK-24) has been isolated , which excretes up to 20.2 mumol of fixed N2 as NH4+ per mg of cell protein in 24 hr at room temperature. Biochemical analysis shows that this mutant, which requires glutamate for growth, releases fixed N2 as NH4+ into the environment because of (i) constitutive synthesis of nitrogenase and (ii) genetic blocks resulting in losses of glutamate synthase [L-glutamine:2-oxoglutarate aminotransferase (NADPH oxidizing), EC 2.6.1.53] and glutamate dehydrogenase [L-glutamate:NADP oxidoreductase (deaminating), EC 1.4.1.4] activities, enzymes essential for NH4+ assimilation into cell material. The parent strain (asm-1), missing only glutamate synthase activity, also actively excretes NH4+ during early phases of its growth but eventually reutilizes the NN4+. A miximum yield of 4.0 mumol of NH4+/ml per 24 hr has been noted for asm-1 only during the growth period. Biosynthesis of NH4+ PROCEEDS AT THE EXPENSE OF A Variety of fermentable sugars, such as sucrose or glucose, with a maximum energy conversion efficiency of about 5 glucose degraded per NH4+ formed. The use of microbes for production of NH4+ fertilizer is discussed.", "contents": "Microbial production of ammonium ion from nitrogen. Genetic manipulation of nitrogenase and key glutamate-forming enzymes can provide mutants that excrete fixed N2 as NH4+. A derepressed N2 fxation mutant (SK-24) has been isolated , which excretes up to 20.2 mumol of fixed N2 as NH4+ per mg of cell protein in 24 hr at room temperature. Biochemical analysis shows that this mutant, which requires glutamate for growth, releases fixed N2 as NH4+ into the environment because of (i) constitutive synthesis of nitrogenase and (ii) genetic blocks resulting in losses of glutamate synthase [L-glutamine:2-oxoglutarate aminotransferase (NADPH oxidizing), EC 2.6.1.53] and glutamate dehydrogenase [L-glutamate:NADP oxidoreductase (deaminating), EC 1.4.1.4] activities, enzymes essential for NH4+ assimilation into cell material. The parent strain (asm-1), missing only glutamate synthase activity, also actively excretes NH4+ during early phases of its growth but eventually reutilizes the NN4+. A miximum yield of 4.0 mumol of NH4+/ml per 24 hr has been noted for asm-1 only during the growth period. Biosynthesis of NH4+ PROCEEDS AT THE EXPENSE OF A Variety of fermentable sugars, such as sucrose or glucose, with a maximum energy conversion efficiency of about 5 glucose degraded per NH4+ formed. The use of microbes for production of NH4+ fertilizer is discussed."}
{"id": "PMID:1090931", "title": "Proteolytic cleavage of bacteriophage lambda repressor in induction.", "content": "The bacteriophage lambda repressor, a protein that maintains the lysogenic state of a bacterium containing a lambda prophage, is cleaved when the lysogen is induced by mitomycin C or ultraviolet light. This cleavage does not occur when induction is prevented by mutational alteration either of the phage repressor or of the host recA gene product. Proteolytic cleavage may be the primary mechanism of repressor inactivation in this induction pathway, or it may follow a different event which causes the initial inactivation.", "contents": "Proteolytic cleavage of bacteriophage lambda repressor in induction. The bacteriophage lambda repressor, a protein that maintains the lysogenic state of a bacterium containing a lambda prophage, is cleaved when the lysogen is induced by mitomycin C or ultraviolet light. This cleavage does not occur when induction is prevented by mutational alteration either of the phage repressor or of the host recA gene product. Proteolytic cleavage may be the primary mechanism of repressor inactivation in this induction pathway, or it may follow a different event which causes the initial inactivation."}
{"id": "PMID:1090932", "title": "DNA strand breaks measured within 100 milliseconds of irradiation of Escherichia coli by 4 MeV electrons.", "content": "A method was developed in which E. coli cells were irradiated with four MeV electrons and transferred to alkaline detergent within a fraction of a second. This technique minimizes the amount of repair of radiation damage before analysis without the necessity of using physical or chemical treatments to inhibit repair and alter the physiological condition of the cells. The yield of DNA strans breaks formed in covalent circular superhelical lambda DNA molecules superinfecting E. coli lysogens was about 4-fold greater when the cells were irradiated in oxygen than when they were irradiated under nitrogen anoxia. The same yields were obtained in phosphate buffer at 3 degrees and 22 degrees as well as in growth medium at 37 degrees, and the yields were not altered by the polA1 mutation. When E. coli lysogenic cells superinfected with lambda were irradiated with doses sufficient to introduce at least seven breaks in the phage DNA, the chromosomal DNA and the superinfecting phage DNA sedimented similarly in alkaline sucrose gradients, indicating that both DNAs were broken to a similar extent during irradiation. However, the yield of breaks calculated for chromosomal DNA in similar experiments was greater than the yield calculated from the first break introduced into covalent circular lambda DNA molecules. These apparently contradictory results are explicable either if the initial break in a superhelical molecule occurs with an efficiency different from that for subsequent breaks, or if the pulsed electron radiation produces a high proportion of double-strand breaks.", "contents": "DNA strand breaks measured within 100 milliseconds of irradiation of Escherichia coli by 4 MeV electrons. A method was developed in which E. coli cells were irradiated with four MeV electrons and transferred to alkaline detergent within a fraction of a second. This technique minimizes the amount of repair of radiation damage before analysis without the necessity of using physical or chemical treatments to inhibit repair and alter the physiological condition of the cells. The yield of DNA strans breaks formed in covalent circular superhelical lambda DNA molecules superinfecting E. coli lysogens was about 4-fold greater when the cells were irradiated in oxygen than when they were irradiated under nitrogen anoxia. The same yields were obtained in phosphate buffer at 3 degrees and 22 degrees as well as in growth medium at 37 degrees, and the yields were not altered by the polA1 mutation. When E. coli lysogenic cells superinfected with lambda were irradiated with doses sufficient to introduce at least seven breaks in the phage DNA, the chromosomal DNA and the superinfecting phage DNA sedimented similarly in alkaline sucrose gradients, indicating that both DNAs were broken to a similar extent during irradiation. However, the yield of breaks calculated for chromosomal DNA in similar experiments was greater than the yield calculated from the first break introduced into covalent circular lambda DNA molecules. These apparently contradictory results are explicable either if the initial break in a superhelical molecule occurs with an efficiency different from that for subsequent breaks, or if the pulsed electron radiation produces a high proportion of double-strand breaks."}
{"id": "PMID:1090933", "title": "Chromatin as a template for RNA synthesis in vitro.", "content": "RNA transcribed in vitro from myeloblast chromatin by exogenously added RNA polymerase B predominantly consists of short chains that remain in hybrid structure with the template; the remainder of the product is free RNA of heterogeneous size. Addition of polyanions during synthesis caused an increase in the size and amount of free RNA with a concomitant decrease in the proportion of small RNA. The large molecular weight RNA is derived from the short RNA chains, which are synthesized de novo during the reaction in vitro. The effect of polyanions on the size and nature of the product may be related to structural changes induced in the template rather than to an inhibition of nuclease activity.", "contents": "Chromatin as a template for RNA synthesis in vitro. RNA transcribed in vitro from myeloblast chromatin by exogenously added RNA polymerase B predominantly consists of short chains that remain in hybrid structure with the template; the remainder of the product is free RNA of heterogeneous size. Addition of polyanions during synthesis caused an increase in the size and amount of free RNA with a concomitant decrease in the proportion of small RNA. The large molecular weight RNA is derived from the short RNA chains, which are synthesized de novo during the reaction in vitro. The effect of polyanions on the size and nature of the product may be related to structural changes induced in the template rather than to an inhibition of nuclease activity."}
{"id": "PMID:1090934", "title": "A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation.", "content": "A new technique, which utilizes the chemical reaction between [32P]diimidazolidate of orthophosphate and the cetyltrimethylammonium salt of high-molecular-weight RNA in nonaqueous dimethyl formamide, has been developed for the 32P-labeling of RNAs after isolation. The radioactive label of high specific activity is introduced onto a phosphorylated 5'-end of the RNA and renders it suitable for 5'-terminal group analysis. When the labeling reaction was applied to the 70S RNA of avian myeloblastosis virus, a labeled 35S RNA was isolated on sucrose-dimethyl sulfoxide gradients without apparent degradation.", "contents": "A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation. A new technique, which utilizes the chemical reaction between [32P]diimidazolidate of orthophosphate and the cetyltrimethylammonium salt of high-molecular-weight RNA in nonaqueous dimethyl formamide, has been developed for the 32P-labeling of RNAs after isolation. The radioactive label of high specific activity is introduced onto a phosphorylated 5'-end of the RNA and renders it suitable for 5'-terminal group analysis. When the labeling reaction was applied to the 70S RNA of avian myeloblastosis virus, a labeled 35S RNA was isolated on sucrose-dimethyl sulfoxide gradients without apparent degradation."}
{"id": "PMID:1090935", "title": "Temperature-sensitive cell mutations that inhibit adenovirus 2 replication.", "content": "Five temperature-sensitive growth mutants of the hamster cell line BHK-21 were tested for the ability to support adenovirus 2 multiplication at 39 degrees and 33 degrees. Wild-type BHK-21 and mutants ts 422E and ts BCH yielded comparable amounts of virus at 33 degrees and 39 degrees, whereas in three other mutants, ts T22, ts T23, and ts AF8, virus production at 39 degrees was reduced to about 1% of that at 33 degrees. Virus yield in the three mutants was not reduced because of a delay in virus production; for all cells tested maximal virus yield at 39 degrees was obtained by 40-50 hr after infection. Normal yields of infectious virus were not obtained from ts AF8 even with a very high multiplicity of infection. In contrast, the virus yield from ts T22 and ts T23 was multiplicity-dependent. Shiftup experiments demonstrated that in ts AF8, a cell cycle mutant which at 39 degrees becomes arrested in G1, virus multiplication was thermosensitive for the first 40 hr of infection. In ts T22 and ts T23, the thermosensitivity was only for the first 3-4 hr of the infection. In all three mutants viral DNA synthesis was reduced by at least 95% at the higher temperature. The cell function specified by the ts AF8 mutation seems to be required for the early period of adenovirus 2 replication, after virus entry into the cell but before the onset of viral DNA replication.", "contents": "Temperature-sensitive cell mutations that inhibit adenovirus 2 replication. Five temperature-sensitive growth mutants of the hamster cell line BHK-21 were tested for the ability to support adenovirus 2 multiplication at 39 degrees and 33 degrees. Wild-type BHK-21 and mutants ts 422E and ts BCH yielded comparable amounts of virus at 33 degrees and 39 degrees, whereas in three other mutants, ts T22, ts T23, and ts AF8, virus production at 39 degrees was reduced to about 1% of that at 33 degrees. Virus yield in the three mutants was not reduced because of a delay in virus production; for all cells tested maximal virus yield at 39 degrees was obtained by 40-50 hr after infection. Normal yields of infectious virus were not obtained from ts AF8 even with a very high multiplicity of infection. In contrast, the virus yield from ts T22 and ts T23 was multiplicity-dependent. Shiftup experiments demonstrated that in ts AF8, a cell cycle mutant which at 39 degrees becomes arrested in G1, virus multiplication was thermosensitive for the first 40 hr of infection. In ts T22 and ts T23, the thermosensitivity was only for the first 3-4 hr of the infection. In all three mutants viral DNA synthesis was reduced by at least 95% at the higher temperature. The cell function specified by the ts AF8 mutation seems to be required for the early period of adenovirus 2 replication, after virus entry into the cell but before the onset of viral DNA replication."}
{"id": "PMID:1090936", "title": "Complementation in cytoplasmic petite mutants of yeast to form respiratory competent cells.", "content": "Complementation has been observed in cytoplasmic respiratory deficient yeast cells (petites) to yield respiratory competent diploids. This successful demonstration depended on the use of spontaneous petites of recent origin and on crosses involving all possible apirwise combinations between the many different petite isolates of opposite mating type. The possibility of deletion of a single unique region of yeast mitochondrial DNA as the initial lesion in petite formation has been eliminated by using strains isogenic for their mitochondrial DNA.", "contents": "Complementation in cytoplasmic petite mutants of yeast to form respiratory competent cells. Complementation has been observed in cytoplasmic respiratory deficient yeast cells (petites) to yield respiratory competent diploids. This successful demonstration depended on the use of spontaneous petites of recent origin and on crosses involving all possible apirwise combinations between the many different petite isolates of opposite mating type. The possibility of deletion of a single unique region of yeast mitochondrial DNA as the initial lesion in petite formation has been eliminated by using strains isogenic for their mitochondrial DNA."}
{"id": "PMID:1090937", "title": "Regulation of proline catabolism by leucyl,phenylalanyl-tRNA-protein transferase.", "content": "A mutant of Escherichia coli lacking leucyl,phenylalanyl-tRNA:protein leucyltransferase, EC 2.3.2.6) exhibited several abnormal growth characteristics relative to the wild type or a revertant when grown with glycerol as a carbon source. All three strains were auxotrophic for proline. The mutant required higher levels of this amino acid than did the other strains to attain a normal growth yield and metabolized exogenous [14C]proline more rapidly. The greater rate of proline utilization was associated with a 4-fold increase in specific activity of proline oxidase. When glucose rather than glycerol was employed as a carbon source, proline oxidase activity was reduced by catabolite repression and the growth ccharacteristics of the mutant were similar to those of the parental and revertant strains. These results suggest that the mutant growth phenotype is due to an altered rate of proline catabolism and constitue evidence for regulation of a specific metabolic pathway by leucyl,phenylalanyl-tRNA-protein transferase.", "contents": "Regulation of proline catabolism by leucyl,phenylalanyl-tRNA-protein transferase. A mutant of Escherichia coli lacking leucyl,phenylalanyl-tRNA:protein leucyltransferase, EC 2.3.2.6) exhibited several abnormal growth characteristics relative to the wild type or a revertant when grown with glycerol as a carbon source. All three strains were auxotrophic for proline. The mutant required higher levels of this amino acid than did the other strains to attain a normal growth yield and metabolized exogenous [14C]proline more rapidly. The greater rate of proline utilization was associated with a 4-fold increase in specific activity of proline oxidase. When glucose rather than glycerol was employed as a carbon source, proline oxidase activity was reduced by catabolite repression and the growth ccharacteristics of the mutant were similar to those of the parental and revertant strains. These results suggest that the mutant growth phenotype is due to an altered rate of proline catabolism and constitue evidence for regulation of a specific metabolic pathway by leucyl,phenylalanyl-tRNA-protein transferase."}
{"id": "PMID:1090938", "title": "Specialized transducing phages for ribosomal protein genes of Escherichia coli.", "content": "Specialized lambda transducing phages have been isolated carrying approximately half the ribosomal protein genes of E. coli. These phages carry regions of the bacterial chromosome between aroE and fus. The ribosomal protein genes on these phages have been identified by the stimulation of ribosomal protein synthesis in ultraviolet-irradiated bacteria following infection by the transducing phage, and by the in vitro synthesis of ribosomal proteins in a DNA-dependent protein synthesizing system. The results indicate lambdadspcl probably carries at least 22 ribosomal protein genes and lambdadspc2 at least 26 genes. All these genes are clustered between trkA and strA. At least 13 of them have not been previously mapped.", "contents": "Specialized transducing phages for ribosomal protein genes of Escherichia coli. Specialized lambda transducing phages have been isolated carrying approximately half the ribosomal protein genes of E. coli. These phages carry regions of the bacterial chromosome between aroE and fus. The ribosomal protein genes on these phages have been identified by the stimulation of ribosomal protein synthesis in ultraviolet-irradiated bacteria following infection by the transducing phage, and by the in vitro synthesis of ribosomal proteins in a DNA-dependent protein synthesizing system. The results indicate lambdadspcl probably carries at least 22 ribosomal protein genes and lambdadspc2 at least 26 genes. All these genes are clustered between trkA and strA. At least 13 of them have not been previously mapped."}
{"id": "PMID:1090951", "title": "The deltopectoral flap: an anatomical and hemodynamic approach.", "content": "An anatomical and hemodynamic study of the deltopectoral flap in cadaver dissections and clinical cases is described. Anatomically, the deltopectoral flap represents a fusion of 3 vascular regions supplied by 3 different sets of vessels. Hemodynamically, the flap consists of a lateral cutaneous flap nourished by a medial arterial pedicle. The method of choice for delay of a deltopectoral flap is discussed.", "contents": "The deltopectoral flap: an anatomical and hemodynamic approach. An anatomical and hemodynamic study of the deltopectoral flap in cadaver dissections and clinical cases is described. Anatomically, the deltopectoral flap represents a fusion of 3 vascular regions supplied by 3 different sets of vessels. Hemodynamically, the flap consists of a lateral cutaneous flap nourished by a medial arterial pedicle. The method of choice for delay of a deltopectoral flap is discussed."}
{"id": "PMID:1090953", "title": "Large Indian forehead rhinoplasty. Case report.", "content": "The classic Indian rhinoplasty, with a midline forehead flap, was performed on 4 young adult patients with \"cut noses\". The rather large flaps survived in all cases, without a delay procedure, and the results were surprisingly good. The appearance of the donor area is, however, a problem-especially in patients with pigmented skins. The method is relatively simple and we believe it to be of value in selected cases.", "contents": "Large Indian forehead rhinoplasty. Case report. The classic Indian rhinoplasty, with a midline forehead flap, was performed on 4 young adult patients with \"cut noses\". The rather large flaps survived in all cases, without a delay procedure, and the results were surprisingly good. The appearance of the donor area is, however, a problem-especially in patients with pigmented skins. The method is relatively simple and we believe it to be of value in selected cases."}
{"id": "PMID:1090956", "title": "Scalping injury.", "content": "The history of scalp avulsions is presented, and one case is reported. A treatment plan for scalped patients is outlined.", "contents": "Scalping injury. The history of scalp avulsions is presented, and one case is reported. A treatment plan for scalped patients is outlined."}
{"id": "PMID:1090958", "title": "Use of parieto-occipital flaps in the surgical treatment of baldness.", "content": "I present a surgical procedure for the treatment of baldness, using parieto-occipital and occipito-parietal flaps. I believe these flaps are somewhat different from those advocated earlier by others, 1,3-5. We have treated about 400 cases (Tables I, II), and the overall results have been excellent with this method. The only complications have been dehiscences of the donor wound when the wound was under tension (15) and necrosis of the distal two cm of the flap (10).", "contents": "Use of parieto-occipital flaps in the surgical treatment of baldness. I present a surgical procedure for the treatment of baldness, using parieto-occipital and occipito-parietal flaps. I believe these flaps are somewhat different from those advocated earlier by others, 1,3-5. We have treated about 400 cases (Tables I, II), and the overall results have been excellent with this method. The only complications have been dehiscences of the donor wound when the wound was under tension (15) and necrosis of the distal two cm of the flap (10)."}
{"id": "PMID:1090959", "title": "The effect of commonly used antiseptics on wound healing.", "content": "Acetic acid, hydrogen peroxide, and povidone-iodine solutions were applied to experimental wounds in rats and to human donor sites to test their effects on wound healing. Control donor sites were treated with saline or dry Owens gauze. The acetic acid and povidone-iodine solutions had no significant gross or microscopic effect on the wounds. The hydrogen peroxide solution seemed to hasten the separation of the scab and to shorten the healing time, though characteristic bullae and ulceration appeared if the hydrogen peroxide treatment was applied after the crust had separated, when new epithelium was visible. We believe that the use of hydrogen peroxide should be avoided after crust separation. When only dry Owens gauze was used to treat split-skin graft donor areas, the result was a 3-day prolongation of the scab separation (compared to the saline controls) and greater subepidermal reactive and inflammatory changes.", "contents": "The effect of commonly used antiseptics on wound healing. Acetic acid, hydrogen peroxide, and povidone-iodine solutions were applied to experimental wounds in rats and to human donor sites to test their effects on wound healing. Control donor sites were treated with saline or dry Owens gauze. The acetic acid and povidone-iodine solutions had no significant gross or microscopic effect on the wounds. The hydrogen peroxide solution seemed to hasten the separation of the scab and to shorten the healing time, though characteristic bullae and ulceration appeared if the hydrogen peroxide treatment was applied after the crust had separated, when new epithelium was visible. We believe that the use of hydrogen peroxide should be avoided after crust separation. When only dry Owens gauze was used to treat split-skin graft donor areas, the result was a 3-day prolongation of the scab separation (compared to the saline controls) and greater subepidermal reactive and inflammatory changes."}
{"id": "PMID:1090960", "title": "Autotransplant of omentum to cover large denudation of the scalp. Case report.", "content": "Omentum was transplanted and covered with a split-skin graft, for repair of a denuding injury of the scalp in a 5-year-old girl. With the many successful recent reports of free skin flaps, the indications for omental transplantation have decreased somewhat, but some peculiar situations may require it.", "contents": "Autotransplant of omentum to cover large denudation of the scalp. Case report. Omentum was transplanted and covered with a split-skin graft, for repair of a denuding injury of the scalp in a 5-year-old girl. With the many successful recent reports of free skin flaps, the indications for omental transplantation have decreased somewhat, but some peculiar situations may require it."}
{"id": "PMID:1090967", "title": "Double-blind procedure: an assessment in a study of lithium prophylaxis.", "content": "Fifty-seven patients with primary affective disorder who were in a double-blind outpatient study to evaluate the prophylaxis of lithium were questioned as to whether they believed they were receiving lithium or placebo. Research nurses who were 'blind' to the patients' medication and a close relative living with each patient were also questioned. Nearly all patients (96%) said they beleived they were receiving lithium, ascribing this belief in 63% of the cases to a perceived improvement in condition. In only 14% of the cases on lithium was the presence of side-effects implicated in the patients' beliefs. One of the three nurses had a correct guess rate in excess of chance expectancy. All nurses tended to be more accurate in their guesses in the cases of patients who had been in prophylactic trials of over 15 months' duration than for patients with briefer periods in the study. Patients' relatives were, as a group, extremely accurate, their correct guess rate exceeding chance expectancy at the 0-001 level.", "contents": "Double-blind procedure: an assessment in a study of lithium prophylaxis. Fifty-seven patients with primary affective disorder who were in a double-blind outpatient study to evaluate the prophylaxis of lithium were questioned as to whether they believed they were receiving lithium or placebo. Research nurses who were 'blind' to the patients' medication and a close relative living with each patient were also questioned. Nearly all patients (96%) said they beleived they were receiving lithium, ascribing this belief in 63% of the cases to a perceived improvement in condition. In only 14% of the cases on lithium was the presence of side-effects implicated in the patients' beliefs. One of the three nurses had a correct guess rate in excess of chance expectancy. All nurses tended to be more accurate in their guesses in the cases of patients who had been in prophylactic trials of over 15 months' duration than for patients with briefer periods in the study. Patients' relatives were, as a group, extremely accurate, their correct guess rate exceeding chance expectancy at the 0-001 level."}
{"id": "PMID:1090970", "title": "Melorheostosis with linear sclerodermatous skin changes.", "content": "A case of melorheostosis with overlying linear skin changes is reported. The radiographic appearance is that of a linear hyperostosis which appears to flow along the cortex of the bone. This is believed to be the twelfth such case in the literature.", "contents": "Melorheostosis with linear sclerodermatous skin changes. A case of melorheostosis with overlying linear skin changes is reported. The radiographic appearance is that of a linear hyperostosis which appears to flow along the cortex of the bone. This is believed to be the twelfth such case in the literature."}
{"id": "PMID:1090971", "title": "Gastric pseudo-ulcers: membrana angularis and pyloric torus defects.", "content": "The membrana angularis and pyloric torus defects are two physiologic bulges which can simulate ulcerations along the lesser curvature of the stomach. The muscular anatomy of the stomach and the mechanism which produces these pseudo-ulcers are discussed. Both pseudoniches can be seen transiently in normal individuals but occasionally are such prominence as to become diagnostic pitfalls. The features and significance of each pseudo-ulcer are reviewed in an attempt to facilitate recognition on the upper gastrointestinal barium examination.", "contents": "Gastric pseudo-ulcers: membrana angularis and pyloric torus defects. The membrana angularis and pyloric torus defects are two physiologic bulges which can simulate ulcerations along the lesser curvature of the stomach. The muscular anatomy of the stomach and the mechanism which produces these pseudo-ulcers are discussed. Both pseudoniches can be seen transiently in normal individuals but occasionally are such prominence as to become diagnostic pitfalls. The features and significance of each pseudo-ulcer are reviewed in an attempt to facilitate recognition on the upper gastrointestinal barium examination."}
{"id": "PMID:1090972", "title": "Angiography of renal transplant using ipsilateral femoral artery compression.", "content": "A technique is described for performing pelvic renal transplant arteriography using compression of the common femoral artery on the same side as the transplant. This method is an alternative to selective catherization.", "contents": "Angiography of renal transplant using ipsilateral femoral artery compression. A technique is described for performing pelvic renal transplant arteriography using compression of the common femoral artery on the same side as the transplant. This method is an alternative to selective catherization."}
{"id": "PMID:1090968", "title": "Radiological concepts of cholecystokinin cholecystography.", "content": "Cholecystokinin-cholecystography originated in Stockholm in 1955. At the present time the radiological procedure is conducted under supervision of medical research teams in the United States. The cholecystokininpancreozymin preparation is imported for clinical investigation under \"Investigational Drug\" regulations. Dr. Donald Hanscom, gastroenterologist, who is conducting clinical investigations in the Midwest, submitted radiographs, publications and evaluations from his files for this manuscript. This gastrointestinal hormone \"squeezes out a diagnosis\" as it is observed fluoroscopically. Dr. LeRoy B. Garbe, radiologist, evaluates these findings in the cases presented. The effects of cholecystokinin on the contrast-filled gall bladder give enhanced diagnostic information for the patient suffering with chronic cholecystitis and cystic ductitis.", "contents": "Radiological concepts of cholecystokinin cholecystography. Cholecystokinin-cholecystography originated in Stockholm in 1955. At the present time the radiological procedure is conducted under supervision of medical research teams in the United States. The cholecystokininpancreozymin preparation is imported for clinical investigation under \"Investigational Drug\" regulations. Dr. Donald Hanscom, gastroenterologist, who is conducting clinical investigations in the Midwest, submitted radiographs, publications and evaluations from his files for this manuscript. This gastrointestinal hormone \"squeezes out a diagnosis\" as it is observed fluoroscopically. Dr. LeRoy B. Garbe, radiologist, evaluates these findings in the cases presented. The effects of cholecystokinin on the contrast-filled gall bladder give enhanced diagnostic information for the patient suffering with chronic cholecystitis and cystic ductitis."}
{"id": "PMID:1090969", "title": "Where we stand on proficiency testing.", "content": "Because of the lack of comprehensive information in radiologic technology publications on the history, purpose and future implications of a process technologists hear talked about as \"proficiency testing,\" and because of a lack of understanding of the role The American Society of Radiologic Technologists is playing in the project, the authors responded to a request by the editor to prepare this article.  Since evaluation of the proficiency testing concept is still going on, this article is only a beginning and cannot be conclusive as to possible implementation, but it serves to provide substantial understanding through which the reader can evaluate future information.", "contents": "Where we stand on proficiency testing. Because of the lack of comprehensive information in radiologic technology publications on the history, purpose and future implications of a process technologists hear talked about as \"proficiency testing,\" and because of a lack of understanding of the role The American Society of Radiologic Technologists is playing in the project, the authors responded to a request by the editor to prepare this article.  Since evaluation of the proficiency testing concept is still going on, this article is only a beginning and cannot be conclusive as to possible implementation, but it serves to provide substantial understanding through which the reader can evaluate future information."}
{"id": "PMID:1090975", "title": "Arteriographic bleeding: a new sign.", "content": "A new sign of arteriographic bleeding, diastolic clearance of angiographic contrast medium selectively from the bleeding vessel, is described. The use of this sign must be confined to rapidly bleeding end-vessel distributions or when nonopacified collateral circulation is not a consideration. It should not be confused with the normal washout of contrast substance in areas of multiple parent vessel collateral circulation where intermittent opacification is a normal feature.", "contents": "Arteriographic bleeding: a new sign. A new sign of arteriographic bleeding, diastolic clearance of angiographic contrast medium selectively from the bleeding vessel, is described. The use of this sign must be confined to rapidly bleeding end-vessel distributions or when nonopacified collateral circulation is not a consideration. It should not be confused with the normal washout of contrast substance in areas of multiple parent vessel collateral circulation where intermittent opacification is a normal feature."}
{"id": "PMID:1090976", "title": "Ultrasonographic determination of the splenic size and its clinical usefulness in various liver diseases.", "content": "Contact ultrasonic tomography was employed to obtain the size of the spleen in vivo. The scanning was done parallel to the ribs, and the splenotomograms obtained by this method showed three basic patterns of enlargement: a soft, bulging spleen, splenic congestion, or a firm spleen. Of the 96 patients with liver disease studied by this technique, 95% gave evidence of splenic enlargement. Useful information on prognosis may be obtained by observing the changes in the sectional areas of the spleen.", "contents": "Ultrasonographic determination of the splenic size and its clinical usefulness in various liver diseases. Contact ultrasonic tomography was employed to obtain the size of the spleen in vivo. The scanning was done parallel to the ribs, and the splenotomograms obtained by this method showed three basic patterns of enlargement: a soft, bulging spleen, splenic congestion, or a firm spleen. Of the 96 patients with liver disease studied by this technique, 95% gave evidence of splenic enlargement. Useful information on prognosis may be obtained by observing the changes in the sectional areas of the spleen."}
{"id": "PMID:1090977", "title": "Detection of canine kidney allograft rejection with 51Cr-labeled lymphocytes.", "content": "Mongrel dogs received transplanted kidneys following total nephrectomy. The whole blood was centrifuged to separate out the lymphocytic fraction, which was tagged with 51Cr. Renal accumulation of 51Cr was followed using imaging detectors. All allograft rejections were accompanied by a simultaneous rise in the count rate. Labeled lymphocytes can be used to detect canine renal rejection, and the authors feel that this technique can be applied to man.", "contents": "Detection of canine kidney allograft rejection with 51Cr-labeled lymphocytes. Mongrel dogs received transplanted kidneys following total nephrectomy. The whole blood was centrifuged to separate out the lymphocytic fraction, which was tagged with 51Cr. Renal accumulation of 51Cr was followed using imaging detectors. All allograft rejections were accompanied by a simultaneous rise in the count rate. Labeled lymphocytes can be used to detect canine renal rejection, and the authors feel that this technique can be applied to man."}
{"id": "PMID:1090978", "title": "The effect of contrast media on patient motion during cerebral angiography.", "content": "Conray, Renografin-60, and Reno-M-60 were compared in 40 patients undergoing cerebral angiography. Pain during injection, as evidenced by head motion, was documented using subtraction. Conray produced less had head motion and therefore a superior subtraction study than either Renografin-60 or Reno-M-60 and was also subjectively the least painful of the three.", "contents": "The effect of contrast media on patient motion during cerebral angiography. Conray, Renografin-60, and Reno-M-60 were compared in 40 patients undergoing cerebral angiography. Pain during injection, as evidenced by head motion, was documented using subtraction. Conray produced less had head motion and therefore a superior subtraction study than either Renografin-60 or Reno-M-60 and was also subjectively the least painful of the three."}
{"id": "PMID:1090979", "title": "The effect of glucagon on barium-enema examination.", "content": "Fifty barium-enema studies were performed with glucagon and 50 with a placebo to compare their effect on colonic spasm, patient discomfort, and diagnostic quality. Each drug was administered in a randomized double-blind fashion and was injected intramuscularly 10 minutes before beginning the enema. Bowel relaxation during fluoroscopy was graded. Patients were questioned about discomfort during and immediately after the enema, and radiographs were reviewed blindly for diagnostic quality and degree of spasm. Studies done with glucagon produced significantly less spasm and discomfort and better diagnostic quality compared to the placebo (p less than 0.01).", "contents": "The effect of glucagon on barium-enema examination. Fifty barium-enema studies were performed with glucagon and 50 with a placebo to compare their effect on colonic spasm, patient discomfort, and diagnostic quality. Each drug was administered in a randomized double-blind fashion and was injected intramuscularly 10 minutes before beginning the enema. Bowel relaxation during fluoroscopy was graded. Patients were questioned about discomfort during and immediately after the enema, and radiographs were reviewed blindly for diagnostic quality and degree of spasm. Studies done with glucagon produced significantly less spasm and discomfort and better diagnostic quality compared to the placebo (p less than 0.01)."}
{"id": "PMID:1090980", "title": "Releif of severe fecal impactions with water-soluble contrast enemas.", "content": "High fecal impaction resulted in complete bowel obstruction in 2 children following renal transplantation and in one adult on chemotherapy and narcotics. The usual methods of relief failed, and water-soluble contrast enemas with a high osmolality were employed. Fluoroscopic control assured placement of the enema fluid at the site of obstruction, where it was very effective in relieving the impaction. It is suggested that such high-osmolality enemas be considered in patients with severe high fecal impactions before surgery is contemplated.", "contents": "Releif of severe fecal impactions with water-soluble contrast enemas. High fecal impaction resulted in complete bowel obstruction in 2 children following renal transplantation and in one adult on chemotherapy and narcotics. The usual methods of relief failed, and water-soluble contrast enemas with a high osmolality were employed. Fluoroscopic control assured placement of the enema fluid at the site of obstruction, where it was very effective in relieving the impaction. It is suggested that such high-osmolality enemas be considered in patients with severe high fecal impactions before surgery is contemplated."}
{"id": "PMID:1090984", "title": "The effect of bithionol sulphoxide on Echinococcus granulosus and Taenia hydatigena infections in dogs.", "content": "Double Thiry-Vella loops in calves were used to show that when heat stable E coli enterotoxin was in contact with only one of the loops, a net increase in water secretion occured in both. It was concluded that either enterotoxin itself or a mediator substance was absorbed and acted via the systemic circulation.", "contents": "The effect of bithionol sulphoxide on Echinococcus granulosus and Taenia hydatigena infections in dogs. Double Thiry-Vella loops in calves were used to show that when heat stable E coli enterotoxin was in contact with only one of the loops, a net increase in water secretion occured in both. It was concluded that either enterotoxin itself or a mediator substance was absorbed and acted via the systemic circulation."}
{"id": "PMID:1090985", "title": "The effects of Escherichia coli endotoxin on the concentrations of mineral elements in the plasma of the domestic fowl.", "content": "The intravenous injection of E coli endotoxin (serogroup 0111 : B4) into eight to nine week old disease-free fowls (0-025-3-0 mg/kg) produced successive falls in the plasma potassium and calcium levels during the 9 h following the injection. This change in the potassium concentration was accompanied by a slight rise in that of sodium, and in some cases a slight rise in that of sodium, and in some cases a slight reduction in the magnesium concentration was detected when that of calcium was reduced. No effect was observed 24 h after the injection. The metal-binding properties of endotoxin, its affinity for membranes and its effect on the secretion of adrenocortical hormones may be involved in the production of these responses. Their pathophysiological significance is also discussed.", "contents": "The effects of Escherichia coli endotoxin on the concentrations of mineral elements in the plasma of the domestic fowl. The intravenous injection of E coli endotoxin (serogroup 0111 : B4) into eight to nine week old disease-free fowls (0-025-3-0 mg/kg) produced successive falls in the plasma potassium and calcium levels during the 9 h following the injection. This change in the potassium concentration was accompanied by a slight rise in that of sodium, and in some cases a slight rise in that of sodium, and in some cases a slight reduction in the magnesium concentration was detected when that of calcium was reduced. No effect was observed 24 h after the injection. The metal-binding properties of endotoxin, its affinity for membranes and its effect on the secretion of adrenocortical hormones may be involved in the production of these responses. Their pathophysiological significance is also discussed."}
{"id": "PMID:1090986", "title": "Studies on the development of chloramphenicol resistance in Salmonella typhimurium.", "content": "Chloramphenicol therapy of acute and chronic Salmonella typhimurium infection in mice did not lead to the development of chloramphenicol resistant mutants of this organism. However, chloramphenicol resistant organisms were readily produced in vitro. Transfer of chloramphenicol resistance from a donor strain of Escherichia coli X12 to a suitable recipient strain of S typhimurium 5235 occurred readily in the intestine of 15 out of 20 conventional mice, following oral administration of large doses of these strains supplemented by chloramphenicol therapy. When a similar system was used in untreated animals, only a small number of chloramphenicol resistant S typhimurium were isolated from three out of 18 mice. The virulence of chloramphenicol resistant S typhimurium produced in vitro and in vivo was similar to that of the sensitive parent strain.", "contents": "Studies on the development of chloramphenicol resistance in Salmonella typhimurium. Chloramphenicol therapy of acute and chronic Salmonella typhimurium infection in mice did not lead to the development of chloramphenicol resistant mutants of this organism. However, chloramphenicol resistant organisms were readily produced in vitro. Transfer of chloramphenicol resistance from a donor strain of Escherichia coli X12 to a suitable recipient strain of S typhimurium 5235 occurred readily in the intestine of 15 out of 20 conventional mice, following oral administration of large doses of these strains supplemented by chloramphenicol therapy. When a similar system was used in untreated animals, only a small number of chloramphenicol resistant S typhimurium were isolated from three out of 18 mice. The virulence of chloramphenicol resistant S typhimurium produced in vitro and in vivo was similar to that of the sensitive parent strain."}
{"id": "PMID:1090987", "title": "Respiratory and cough mechanics in antitussive trials. Responsivity of objective indices to the treatment of acute upper respiratory tract infections.", "content": "The relative responsitivy of nine expirographic and airway indices to antitussive therapy was studied in a randomized double-blind trial in which 60 adults without bronchopulmonary disorders, but suffering from common colds, were given oral doses of five drug formulations or placebo during their initial 48 h of symptoms. Thoracic gas volume (Vtg), nasal (Rn), lower (Ra), total t[Rn + Ra]) and specific (sRa) airway resistances were determined plethysmographically; forced vital capacity (FVC), forced expiratory volume for the first second (FEV1.0) and maximal mid-expiratory flow rate (MMEFR) were recorded spirometrically. Cough pressure at peak flow furnished an index of cough (CI). The measurements were made at baseline and for 4 h after drug; medication was continued for two additional doses and the indices determined again the following morning. Abnormal control means were noted for Rn and [Rn + Ra] (90.8%), cough resistance (83.3%), MMEFR (58.3%), Ra and sRa (30.0%), but less ofter for FEV1.0, Vtg and FVC (6.6, 5.1 and 0.0%, respectively). Nasal and total airways flow resistance means were as sensitive to active therapy and in discriminating the relative worth of medication as direct CI values, Ra and sRa were less reliable, and the three spirometric functions were the lease responsive. This ranking was consistent with the common central airways location of subepithelial receptors responsible for cough and the associated reflex bronchoconstriction. The greatest changes in cough resistance followed treatment with the most significant activity in the nasal, as well as the lower tract, component of total airway resistance.", "contents": "Respiratory and cough mechanics in antitussive trials. Responsivity of objective indices to the treatment of acute upper respiratory tract infections. The relative responsitivy of nine expirographic and airway indices to antitussive therapy was studied in a randomized double-blind trial in which 60 adults without bronchopulmonary disorders, but suffering from common colds, were given oral doses of five drug formulations or placebo during their initial 48 h of symptoms. Thoracic gas volume (Vtg), nasal (Rn), lower (Ra), total t[Rn + Ra]) and specific (sRa) airway resistances were determined plethysmographically; forced vital capacity (FVC), forced expiratory volume for the first second (FEV1.0) and maximal mid-expiratory flow rate (MMEFR) were recorded spirometrically. Cough pressure at peak flow furnished an index of cough (CI). The measurements were made at baseline and for 4 h after drug; medication was continued for two additional doses and the indices determined again the following morning. Abnormal control means were noted for Rn and [Rn + Ra] (90.8%), cough resistance (83.3%), MMEFR (58.3%), Ra and sRa (30.0%), but less ofter for FEV1.0, Vtg and FVC (6.6, 5.1 and 0.0%, respectively). Nasal and total airways flow resistance means were as sensitive to active therapy and in discriminating the relative worth of medication as direct CI values, Ra and sRa were less reliable, and the three spirometric functions were the lease responsive. This ranking was consistent with the common central airways location of subepithelial receptors responsible for cough and the associated reflex bronchoconstriction. The greatest changes in cough resistance followed treatment with the most significant activity in the nasal, as well as the lower tract, component of total airway resistance."}
{"id": "PMID:1090988", "title": "Aerosol chemotherapy in bronchopulmonary candidiasis.", "content": "One of the most frequent complications encountered in non-specific respiratory pathology of recent years is overinfection by Candida albicans. An important contributive factor is the recent massive antibiotherapy, above all with tetracyclines, favouring this overinfection. Since at present the treatment of bronchopulmonary processes is difficult owing to the lack of an effective oral or parenteral therapy, a study was carried out of 33 patients treated with nystatin and amphotericin B in aerosol form, with 3-4 sessions of treatment per day, during a minimum of 10 days. At each session either 50,000 U of nystatin or 5 mg of amphotericin B were administered. The results obtained showed that after treatment, C. albicans was no longer present in the sputum of 84% of cases treated. In view of these results it is considered that - at present - the two most suitable substances for the treatment of pulmonary candidiases are nystatin and amphotericin B in aerosol form.", "contents": "Aerosol chemotherapy in bronchopulmonary candidiasis. One of the most frequent complications encountered in non-specific respiratory pathology of recent years is overinfection by Candida albicans. An important contributive factor is the recent massive antibiotherapy, above all with tetracyclines, favouring this overinfection. Since at present the treatment of bronchopulmonary processes is difficult owing to the lack of an effective oral or parenteral therapy, a study was carried out of 33 patients treated with nystatin and amphotericin B in aerosol form, with 3-4 sessions of treatment per day, during a minimum of 10 days. At each session either 50,000 U of nystatin or 5 mg of amphotericin B were administered. The results obtained showed that after treatment, C. albicans was no longer present in the sputum of 84% of cases treated. In view of these results it is considered that - at present - the two most suitable substances for the treatment of pulmonary candidiases are nystatin and amphotericin B in aerosol form."}
{"id": "PMID:1090989", "title": "Regional distribution of pulmonary blood flow in normal high-altitude dwellers at 3,650 m (12,200 ft).", "content": "Simultaneous isotope dilution curves were recorded from the right upper (QRUZ) and right lower lung zones (QRLZ) by surface scanning in the sitting and recumbent positions in 15 normal high-altitude-born (HAD) males and in 1 HAD female as well as from 3 male newcomers, using 10 muCi of 131I-HSA as a bolus injection into the right ventricle. Similar information was also obtained at sea level from 5 normal males. The mean percent distribution of total pulmonary blood flow (Q) to RUZ and RLZ in the two body postures indicate (1) that in the vertical position RUZ in males receives about 17% of Q regardless of altitude and elevation in mean pulmonary artery pressure (MPAP) in HAD of 8.6 mm Hg above that extant at sea level; (2) recumbency at high altitude showed QRUZ also to be lower than at sea level; (3) elevation in MPAP at altitude has no significant effect on changing the sea-level distribution pattern of pulmonary blood flow.", "contents": "Regional distribution of pulmonary blood flow in normal high-altitude dwellers at 3,650 m (12,200 ft). Simultaneous isotope dilution curves were recorded from the right upper (QRUZ) and right lower lung zones (QRLZ) by surface scanning in the sitting and recumbent positions in 15 normal high-altitude-born (HAD) males and in 1 HAD female as well as from 3 male newcomers, using 10 muCi of 131I-HSA as a bolus injection into the right ventricle. Similar information was also obtained at sea level from 5 normal males. The mean percent distribution of total pulmonary blood flow (Q) to RUZ and RLZ in the two body postures indicate (1) that in the vertical position RUZ in males receives about 17% of Q regardless of altitude and elevation in mean pulmonary artery pressure (MPAP) in HAD of 8.6 mm Hg above that extant at sea level; (2) recumbency at high altitude showed QRUZ also to be lower than at sea level; (3) elevation in MPAP at altitude has no significant effect on changing the sea-level distribution pattern of pulmonary blood flow."}
{"id": "PMID:1090997", "title": "Blood volume.", "content": "During the past decade the diagnostic use of blood volume determinations has declined as a result of the generation of largely inaccurate results and inappropriate normalization and interpretation. After historical development of more than 50 years, current methodology employs 125I-labeled human serum albumin and 51Cr-labeled red blood cells to determine plasma volume and red cell volume, respectively. Accurate blood volume determinations require (1) abandoning the use of the mean body hematocrit:venous hematocrit ratio and using simultaneous independent measurements of both volumes; (2) delaying multiple postinjection patient samples until complete mixing and equilibration are complete; (3) backextrapolation of plasma concentrations of 125I to account for albumin loss from the plasma, and, rarely, back-extrapolation of red cell concentrations to account for dilution by red cells transfused during the procedure; (4) normalization of volumes by adjusting patient weight to normal correspondence with lean tissue mass, whenever necessary. A rapid, routine method that fulfills these four requirements is presented. A number of surgical and medical conditions in which blood volume determinations are very useful in diagnosis and therapy are discussed. Recently developed techniques for blood volume measurements include neutron acativation analysis and fluorescent excitation analysis. Correct normalization of accurate blood volume measurements will provide a valuable service to the entire medical community.", "contents": "Blood volume. During the past decade the diagnostic use of blood volume determinations has declined as a result of the generation of largely inaccurate results and inappropriate normalization and interpretation. After historical development of more than 50 years, current methodology employs 125I-labeled human serum albumin and 51Cr-labeled red blood cells to determine plasma volume and red cell volume, respectively. Accurate blood volume determinations require (1) abandoning the use of the mean body hematocrit:venous hematocrit ratio and using simultaneous independent measurements of both volumes; (2) delaying multiple postinjection patient samples until complete mixing and equilibration are complete; (3) backextrapolation of plasma concentrations of 125I to account for albumin loss from the plasma, and, rarely, back-extrapolation of red cell concentrations to account for dilution by red cells transfused during the procedure; (4) normalization of volumes by adjusting patient weight to normal correspondence with lean tissue mass, whenever necessary. A rapid, routine method that fulfills these four requirements is presented. A number of surgical and medical conditions in which blood volume determinations are very useful in diagnosis and therapy are discussed. Recently developed techniques for blood volume measurements include neutron acativation analysis and fluorescent excitation analysis. Correct normalization of accurate blood volume measurements will provide a valuable service to the entire medical community."}
{"id": "PMID:1090998", "title": "Use of radioisotope techniques in the clinical evaluation of patients with megaloblastic anemia.", "content": "Because virtually all cases of vitamin B12 deficiency seen in this country are due to malabsorption, the availability of radioactive vitamin B12 for direct measurement of absorption of this essential nutrient has proved to be of great clinical value. These tests are useful not only in demonstrating vitamin B12  malabsorption but also often in defining the pathophysiological mechanism responsible for this abnormality. The urinary excretion test of Schilling remains the most useful test for vitamin B12 absorption. Minor precautions and modifications in technique make the test results more reliable and easier to interpret. The 8-hr plasma test for vitamin B12 absorption can no longer be considered acceptable. Some patients with vitamin B12 malabsorption have results in the normal range when studied by this method. Serum vitamin B12 assays utilizing radioactive vitamin B12 and the isotope dilution principle are not widely used and are useful screening tests. Low normal or borderline results observed in patients with clinical evidence suggestive of vitamin B12 deficiency should be interpreted with caution or confirmed by radioactive vitamin B12 absorption studies. Radioactive vitamin B12 can also be used for rapid, reliable assay of gastric intrinsic factor, antibody to intrinsic factor and unsaturated vitamin B12 serum. Methods using radioactive folate compounds for similar in vivo and in vitro studies are not yet applicable for routine use in nuclear medicine laboratories.", "contents": "Use of radioisotope techniques in the clinical evaluation of patients with megaloblastic anemia. Because virtually all cases of vitamin B12 deficiency seen in this country are due to malabsorption, the availability of radioactive vitamin B12 for direct measurement of absorption of this essential nutrient has proved to be of great clinical value. These tests are useful not only in demonstrating vitamin B12  malabsorption but also often in defining the pathophysiological mechanism responsible for this abnormality. The urinary excretion test of Schilling remains the most useful test for vitamin B12 absorption. Minor precautions and modifications in technique make the test results more reliable and easier to interpret. The 8-hr plasma test for vitamin B12 absorption can no longer be considered acceptable. Some patients with vitamin B12 malabsorption have results in the normal range when studied by this method. Serum vitamin B12 assays utilizing radioactive vitamin B12 and the isotope dilution principle are not widely used and are useful screening tests. Low normal or borderline results observed in patients with clinical evidence suggestive of vitamin B12 deficiency should be interpreted with caution or confirmed by radioactive vitamin B12 absorption studies. Radioactive vitamin B12 can also be used for rapid, reliable assay of gastric intrinsic factor, antibody to intrinsic factor and unsaturated vitamin B12 serum. Methods using radioactive folate compounds for similar in vivo and in vitro studies are not yet applicable for routine use in nuclear medicine laboratories."}
{"id": "PMID:1091001", "title": "Colestipol hydrochloride, a new hypolipidemic drug: a two-year study.", "content": "Colestipol hydrochloride is an insoluble, nonabsorbable copolymer with bile-acid-binding capacity. It prevents reabsorption of cholates from the intestinal tract into the enterohepatic circulation causing a net loss of bile acids, and therefore of cholesterol. Sixty subjects with cholesterol levels over 250 mg/100 ml were studied for 104 weeks. Patients with normal phenotypes, types 2,3, and 4, were given 5 gm three times daily and experienced an average drop of 40 mg/100 ml (14%). While patients with types 2,3, and 4 hyperlipidemia responded effectively, cholesterol levels in type 2 patients dropped earliest and most consistently with an average decrease of 58 mg/100 ml (19%). A comparable group of patients with hyperlipidemia taking placebo showed on average no change in serum cholesterol. Serum triglyceride values were not altered significantly. The resin is not absorbed from the gastrointestinal tract and produces a slight increase in fecal volume. Results of chemistries, enzyme assays, prothrombin times, hematology, and urinalysis and body weights wer unaltered. There was no evidence of lithogenic bile production. Colestipol is a tasteless and ordorless copolymer with high acceptability. Side effects were limited to occasional bloating, gas, and constipation. The drug is a safe, effective, palatable hypolipedmic agent.", "contents": "Colestipol hydrochloride, a new hypolipidemic drug: a two-year study. Colestipol hydrochloride is an insoluble, nonabsorbable copolymer with bile-acid-binding capacity. It prevents reabsorption of cholates from the intestinal tract into the enterohepatic circulation causing a net loss of bile acids, and therefore of cholesterol. Sixty subjects with cholesterol levels over 250 mg/100 ml were studied for 104 weeks. Patients with normal phenotypes, types 2,3, and 4, were given 5 gm three times daily and experienced an average drop of 40 mg/100 ml (14%). While patients with types 2,3, and 4 hyperlipidemia responded effectively, cholesterol levels in type 2 patients dropped earliest and most consistently with an average decrease of 58 mg/100 ml (19%). A comparable group of patients with hyperlipidemia taking placebo showed on average no change in serum cholesterol. Serum triglyceride values were not altered significantly. The resin is not absorbed from the gastrointestinal tract and produces a slight increase in fecal volume. Results of chemistries, enzyme assays, prothrombin times, hematology, and urinalysis and body weights wer unaltered. There was no evidence of lithogenic bile production. Colestipol is a tasteless and ordorless copolymer with high acceptability. Side effects were limited to occasional bloating, gas, and constipation. The drug is a safe, effective, palatable hypolipedmic agent."}
{"id": "PMID:1091002", "title": "Primary prevention of atherosclerosis: a challenge to the physician caring for children.", "content": "This review article stresses the importance of prevention in the reduction of the incidence of artherosclerotic disease, especially coronary heart disease. Evidence accumulated from autopsy studies, animal experimentation, and long-term epidemiologic studies supports the concept that atherosclerosis has its inception in childhood. Adult programs designed to change life styles and attitudes or reverse morphologic changes appear doomed to failure. Therefore, if nutritional, physical activity, and smoking habits are to be altered, it is essential to begin these efforts in infancy and childhood.", "contents": "Primary prevention of atherosclerosis: a challenge to the physician caring for children. This review article stresses the importance of prevention in the reduction of the incidence of artherosclerotic disease, especially coronary heart disease. Evidence accumulated from autopsy studies, animal experimentation, and long-term epidemiologic studies supports the concept that atherosclerosis has its inception in childhood. Adult programs designed to change life styles and attitudes or reverse morphologic changes appear doomed to failure. Therefore, if nutritional, physical activity, and smoking habits are to be altered, it is essential to begin these efforts in infancy and childhood."}
{"id": "PMID:1091003", "title": "Primary prevention of atherosclerosis: nutritional aspects.", "content": "The changes suggested for reducing consumption of calories, cholesterol, and sodium are not radical but sensible. These modifications are nutritionally sound suggestions which will lead to the development of good dietary habits which may be continued throughout life. Hopefully, this will prevent the child from entering adulthood with habits inappropriate for longevity and subsequently having to face the extremely difficult task of changing well-established habits.", "contents": "Primary prevention of atherosclerosis: nutritional aspects. The changes suggested for reducing consumption of calories, cholesterol, and sodium are not radical but sensible. These modifications are nutritionally sound suggestions which will lead to the development of good dietary habits which may be continued throughout life. Hopefully, this will prevent the child from entering adulthood with habits inappropriate for longevity and subsequently having to face the extremely difficult task of changing well-established habits."}
{"id": "PMID:1091004", "title": "Salmonella empyema and Hodgkin's disease.", "content": "Bilateral Salmonella empyema was diagnosed in a 34-year old woman with active stage IV-B Hodgkin's disease and marked depression of immunologic function. Factors which might have predisposed to her infectious complication are discussed. The patient's dramatic response to treatment is stressed.", "contents": "Salmonella empyema and Hodgkin's disease. Bilateral Salmonella empyema was diagnosed in a 34-year old woman with active stage IV-B Hodgkin's disease and marked depression of immunologic function. Factors which might have predisposed to her infectious complication are discussed. The patient's dramatic response to treatment is stressed."}
{"id": "PMID:1091011", "title": "Modern approach to the diagnosis of anaerobic surgical sepsis.", "content": "Oxygen-sensitive anaerobic bacteria comprise the largest group of organisms among the human endogenous microflora. The oral cavity, the vagina, and the colon are the areas of the human body where the obligate anaerobes are predominant and can be isolated in very high numbers. Sepsis following surgery of any of these organs is frequently due to the escape of these endogenous bacteria during the course of the operative procedure. To enable the isolation and successful identification of most of these anaerobic microorganisms from clinical exudate, special collection and bacteriologic techniques are necessary. The acceptable anaerobic collection techniques have been reviewed in this paper. All of these techniques minimize the exposure of the clinical specimen to atmospheric oxygen during collection and transfer. The modern equipment used in sophisticated anaerobic bacteriologic workup has been discussed. Clinical clues that indicate anaerobic sepsis include a putrid odor of the exudate and evidence of abscess, necrosis, or associated gas formation. The importance and value of the gram-stain in the early identification of surgical sepsis has been stressed.", "contents": "Modern approach to the diagnosis of anaerobic surgical sepsis. Oxygen-sensitive anaerobic bacteria comprise the largest group of organisms among the human endogenous microflora. The oral cavity, the vagina, and the colon are the areas of the human body where the obligate anaerobes are predominant and can be isolated in very high numbers. Sepsis following surgery of any of these organs is frequently due to the escape of these endogenous bacteria during the course of the operative procedure. To enable the isolation and successful identification of most of these anaerobic microorganisms from clinical exudate, special collection and bacteriologic techniques are necessary. The acceptable anaerobic collection techniques have been reviewed in this paper. All of these techniques minimize the exposure of the clinical specimen to atmospheric oxygen during collection and transfer. The modern equipment used in sophisticated anaerobic bacteriologic workup has been discussed. Clinical clues that indicate anaerobic sepsis include a putrid odor of the exudate and evidence of abscess, necrosis, or associated gas formation. The importance and value of the gram-stain in the early identification of surgical sepsis has been stressed."}
{"id": "PMID:1091019", "title": "Platelet heparin-neutralizing factor (platelet factor 4).", "content": "The heparin-neutralizing platelet factor (PF4) is released from platelets under the influence of inducers of aggregation and nucleotide-release in the form of a high-molecular weight complex. The heparin-neutralizing activity is carried by a basic protein of molecular weight 29,700, four of which combine with a proteoglycan carrier, which in turn consists of 4 chondroitin sulfate A residues and a peptide moiety.", "contents": "Platelet heparin-neutralizing factor (platelet factor 4). The heparin-neutralizing platelet factor (PF4) is released from platelets under the influence of inducers of aggregation and nucleotide-release in the form of a high-molecular weight complex. The heparin-neutralizing activity is carried by a basic protein of molecular weight 29,700, four of which combine with a proteoglycan carrier, which in turn consists of 4 chondroitin sulfate A residues and a peptide moiety."}
{"id": "PMID:1091025", "title": "A direct visualisation of helicoidal architecture in Carcinus maenas and Halocynthia papillosa by scanning electron microscopy.", "content": "The test of the Tunicate Halocynthia papillosa is shown to have a laminate organization. Three properties of a helicoidal model can be directly observed: (1) Fibres in each lamina run parallel. (2) Fibres in adjacent laminae run at an angle. (3) In oblique section, discontinuous parabolic arcs made up of straight lengths of fibre from succeeding laminae are observed. These properties are confirmed in the cuticle of Carcinus maenas and the relationship of the pore canals to the laminar organization is discussed. It is shown by matching light and electron micrographs that the 'macrofibres' of recent workers are displaced pore canal tubules.", "contents": "A direct visualisation of helicoidal architecture in Carcinus maenas and Halocynthia papillosa by scanning electron microscopy. The test of the Tunicate Halocynthia papillosa is shown to have a laminate organization. Three properties of a helicoidal model can be directly observed: (1) Fibres in each lamina run parallel. (2) Fibres in adjacent laminae run at an angle. (3) In oblique section, discontinuous parabolic arcs made up of straight lengths of fibre from succeeding laminae are observed. These properties are confirmed in the cuticle of Carcinus maenas and the relationship of the pore canals to the laminar organization is discussed. It is shown by matching light and electron micrographs that the 'macrofibres' of recent workers are displaced pore canal tubules."}
{"id": "PMID:1091026", "title": "On the ultrastructure and permeability of taste buds of the marine teleost Ciliata mustela.", "content": "The abundant taste buds of the barbels and free fin rays of the five bearded rockling, Ciliata mustela contain an average of 100-150 cells, falling into two types. Tubule-containing cells ('t-cells'), tentatively identified as receptor cells, and each surrounded by fibril-containing cells ('f-cells') in the central part of the bud. t-Cells also occur in two concentric shells separated by indifferent epithelial cells at the periphery of the bud. f-Cells are characterized by their concentrations of fine fibrils, and by granules or vesicles of 180-190 mmu diameter. The 100 or so receptor cells in a taste bud are innervated by some 250 axons. Lanthanum penetrates more deeply into the extracellular space of taste buds than into the extracellular space of the general epithelium, perhaps indicating that a greater area than the mere protruding tip of receptor cells may be accessible to chemical stimulation. Degenerating cells may provide an important route of entry for such external agents.", "contents": "On the ultrastructure and permeability of taste buds of the marine teleost Ciliata mustela. The abundant taste buds of the barbels and free fin rays of the five bearded rockling, Ciliata mustela contain an average of 100-150 cells, falling into two types. Tubule-containing cells ('t-cells'), tentatively identified as receptor cells, and each surrounded by fibril-containing cells ('f-cells') in the central part of the bud. t-Cells also occur in two concentric shells separated by indifferent epithelial cells at the periphery of the bud. f-Cells are characterized by their concentrations of fine fibrils, and by granules or vesicles of 180-190 mmu diameter. The 100 or so receptor cells in a taste bud are innervated by some 250 axons. Lanthanum penetrates more deeply into the extracellular space of taste buds than into the extracellular space of the general epithelium, perhaps indicating that a greater area than the mere protruding tip of receptor cells may be accessible to chemical stimulation. Degenerating cells may provide an important route of entry for such external agents."}
{"id": "PMID:1091030", "title": "A computerized system for the selection of organ transplant recipients.", "content": "The Southeastern Regional Organ Procurement Program has developed a computerized system for the selection of organ transplant recipients.  This system has proven to be a useful method for the rapid selection of histocompatible recipients for organ transplants from a large pool of potential recipients in a wide geographical area.  In addition, the system provides a readily accessible source of data that can be used to analyze the relationship between histocompatibility and transplant survival.", "contents": "A computerized system for the selection of organ transplant recipients. The Southeastern Regional Organ Procurement Program has developed a computerized system for the selection of organ transplant recipients.  This system has proven to be a useful method for the rapid selection of histocompatible recipients for organ transplants from a large pool of potential recipients in a wide geographical area.  In addition, the system provides a readily accessible source of data that can be used to analyze the relationship between histocompatibility and transplant survival."}
{"id": "PMID:1091031", "title": "Detergent solubilisation of baboon histocompatibility antigens and their use in prolonging liver allograft survival.", "content": "Histocompatibility antigens have been solubilised from baboon spleen cell membranes by brief exposure to sodium deoxycholate (DOC).  Preparations contained a high molecular weight species (DOCH-Ag) excluded by Sephadex G-200, and a low molecular weight species (DOCL-Ag) which eluted in the included fraction (molecular weight 46,000).  The low molecular weight antigen was stable and was not sedimented by high speed centrifugation even after dialysis against a low molar salt solution.  It elicited a delayed-type hypersensitivity response in sensitised baboons but not in normal animals.  DOCL-Ag cultured in vitro with sensitised allogeneic lymphocytes produced a 1.3- to 24.9-fold stimulation, whereas when cultured with normal lymphocytes the maximum stimulation index was 2.  Intravenous administration of donor DOCL-Ag (2 mg/kg) at the time of transplantation and without additional immunosuppression significantly prolonged the mean survival time of baboon liver allografts.  The same treatment with donor DOCH-Ag did not prolong graft survival.", "contents": "Detergent solubilisation of baboon histocompatibility antigens and their use in prolonging liver allograft survival. Histocompatibility antigens have been solubilised from baboon spleen cell membranes by brief exposure to sodium deoxycholate (DOC).  Preparations contained a high molecular weight species (DOCH-Ag) excluded by Sephadex G-200, and a low molecular weight species (DOCL-Ag) which eluted in the included fraction (molecular weight 46,000).  The low molecular weight antigen was stable and was not sedimented by high speed centrifugation even after dialysis against a low molar salt solution.  It elicited a delayed-type hypersensitivity response in sensitised baboons but not in normal animals.  DOCL-Ag cultured in vitro with sensitised allogeneic lymphocytes produced a 1.3- to 24.9-fold stimulation, whereas when cultured with normal lymphocytes the maximum stimulation index was 2.  Intravenous administration of donor DOCL-Ag (2 mg/kg) at the time of transplantation and without additional immunosuppression significantly prolonged the mean survival time of baboon liver allografts.  The same treatment with donor DOCH-Ag did not prolong graft survival."}
{"id": "PMID:1091033", "title": "Prolongation of liver allograft survival by donor-specific soluble transplantation antigens and antigen-antibody complexes.", "content": "Soluble transplantation antigens were prepared from chacma baboon spleen cell membranes by a modified 3 M KCl technique.  The extracts were soluble according to usually accepted criteria.  Molecular weight was 37,700 daltons.  Antigenicity was confirmed by inhibition of cytotoxicity of alloantibody.  The antigens stimulated sensitized lymphocytes in culture but failed to stimulate unsensitized lymphocytes.  Antigen-antibody complexes were assembled in vitro in the regions of equivalence and marked antigen excess.  Administration of a single dose of soluble donor antigen at the time of orthotopic liver allotransplantation prolonged graft survival significantly.  Concomitant administration of a polyspecific alloantibody, previously shown to prolong baboon liver allograft survival, abrogated the graft prolongation produced by the antigen or antibody given alone.  When the administration of the soluble antigen was preceded by a dose of cyclophosphamide (20 mg/kg), graft prolongation was slightly curtailed. Antecedent administration of azathioprine and prednisolone for 3 days seemed to render the antigen immunogenic in that liver allograft rejection was accelerated.  Antigen-antibody complexes at equivalence prolonged liver allograft survival beyond that seen with antigen alone, but complexes in marked antigen excess were immunogenic, inasmuch as graft survival was not prolonged and the histological features were those of accelerated rejection.", "contents": "Prolongation of liver allograft survival by donor-specific soluble transplantation antigens and antigen-antibody complexes. Soluble transplantation antigens were prepared from chacma baboon spleen cell membranes by a modified 3 M KCl technique.  The extracts were soluble according to usually accepted criteria.  Molecular weight was 37,700 daltons.  Antigenicity was confirmed by inhibition of cytotoxicity of alloantibody.  The antigens stimulated sensitized lymphocytes in culture but failed to stimulate unsensitized lymphocytes.  Antigen-antibody complexes were assembled in vitro in the regions of equivalence and marked antigen excess.  Administration of a single dose of soluble donor antigen at the time of orthotopic liver allotransplantation prolonged graft survival significantly.  Concomitant administration of a polyspecific alloantibody, previously shown to prolong baboon liver allograft survival, abrogated the graft prolongation produced by the antigen or antibody given alone.  When the administration of the soluble antigen was preceded by a dose of cyclophosphamide (20 mg/kg), graft prolongation was slightly curtailed. Antecedent administration of azathioprine and prednisolone for 3 days seemed to render the antigen immunogenic in that liver allograft rejection was accelerated.  Antigen-antibody complexes at equivalence prolonged liver allograft survival beyond that seen with antigen alone, but complexes in marked antigen excess were immunogenic, inasmuch as graft survival was not prolonged and the histological features were those of accelerated rejection."}
{"id": "PMID:1091029", "title": "The renal effect of intravenous adenine in humans.", "content": "The purine base, adenine, improves the posttransfusion viability of liquid stored blood. However, adenine in high doses may cause kidney damage becuase of the precipitation in renal tubules of its incoluble metabolite, 2,8-dioxyadenine. Adenine is not licensed for use in the United States because it may be nephrotoxic. In a controlled, randomized, double-blind study, eight human subjects received 10 mg/kg of adenine infused intravenously over one hour, four subjects received 5 mg/kg, and four subjects received no adenine. Renal function tests were performed on each subject before adenine infusion and one day and one week following the infusion. Tests included an assessment of glomerular function (serum creatinine, creatinine clearance, protein excretion), proximal tubular function (amino acid and glucose excretion), and distal tubular function (maximal acidifying and concentrating ability). Plasma and urine levels of adenine and 2,8-dioxyadenine were measured. Renal function tests showed no evidence of kidney damage secondary to adenine.", "contents": "The renal effect of intravenous adenine in humans. The purine base, adenine, improves the posttransfusion viability of liquid stored blood. However, adenine in high doses may cause kidney damage becuase of the precipitation in renal tubules of its incoluble metabolite, 2,8-dioxyadenine. Adenine is not licensed for use in the United States because it may be nephrotoxic. In a controlled, randomized, double-blind study, eight human subjects received 10 mg/kg of adenine infused intravenously over one hour, four subjects received 5 mg/kg, and four subjects received no adenine. Renal function tests were performed on each subject before adenine infusion and one day and one week following the infusion. Tests included an assessment of glomerular function (serum creatinine, creatinine clearance, protein excretion), proximal tubular function (amino acid and glucose excretion), and distal tubular function (maximal acidifying and concentrating ability). Plasma and urine levels of adenine and 2,8-dioxyadenine were measured. Renal function tests showed no evidence of kidney damage secondary to adenine."}
{"id": "PMID:1091036", "title": "Evidence of cytotoxic T and B immunoblasts in the thoracic duct of rats bearing tumour grafts.", "content": "Wistar rats were immunised with allogeneic or xenogeneic tumour before collection of their thoracic duct lymph. Specifically cytotoxic effector cells were found in the lymph between 3 and 8 days after immunisation, and their occurrence coincided with an increased number of immunoblasts in the lymphocyte population. The immune response in lymph to allogeneic cells appeared to be affected solely by radiosensitive thymus-dependent lymphocytes; no complement-dependent killing was evident and cytotoxic cells failed to appear when immunised animals were deprived of thymus-dependent lymphocytes. In contrast, the response to immunisation with xenogeneic cells elicited both complement-dependent and complement-independent cytotoxicity, but only the former could be detected in animals deprived of thymus-dependent lymphocytes. In normal animals and in animals deprived of thymus-dependent cells, the cytotoxic cells in the thoracic duct lymph appeared to be large lymphocytes or immunoblasts.", "contents": "Evidence of cytotoxic T and B immunoblasts in the thoracic duct of rats bearing tumour grafts. Wistar rats were immunised with allogeneic or xenogeneic tumour before collection of their thoracic duct lymph. Specifically cytotoxic effector cells were found in the lymph between 3 and 8 days after immunisation, and their occurrence coincided with an increased number of immunoblasts in the lymphocyte population. The immune response in lymph to allogeneic cells appeared to be affected solely by radiosensitive thymus-dependent lymphocytes; no complement-dependent killing was evident and cytotoxic cells failed to appear when immunised animals were deprived of thymus-dependent lymphocytes. In contrast, the response to immunisation with xenogeneic cells elicited both complement-dependent and complement-independent cytotoxicity, but only the former could be detected in animals deprived of thymus-dependent lymphocytes. In normal animals and in animals deprived of thymus-dependent cells, the cytotoxic cells in the thoracic duct lymph appeared to be large lymphocytes or immunoblasts."}
{"id": "PMID:1091037", "title": "Studies on the specific suppression of renal allograft rejection in presensitised rats. Theoretical and clinical implications.", "content": "DA and Lewis rats were sensitised by two (DA times Lewis)F1 skin allografts 6 weeks apart and were given a (DA times Lewis)F1 renal allograft 4 weeks after the second skin allograft. Passive enhancement was tbsted in both strain combinations, the (DA times Lewis)F1 to DA model representing the weaker histocompatibility barrier as judged by renal allograft rejection. Passive enhancemnt was much less effective in the sensitised weaker model and was sometimes virtually ineffective in the sensitised stronger model. The powerful combination of antilymphocyte serum and enhancing serum could only delay but not prevent rejection in the latter model. Thus, the avoidance of sensitisation might be of critical importance for the clinical applicability of passive enhancement. Furthermore, it was shown that a sensitised cellular immune system is susceptible to specific immunosuppression, but the result depends on the strain combination used and whether the test graft is skin or kidney. A hypothesis is presented to explain the basis for the difference in strength of different histocompatibility barriers and also the differences between skin and kidney allograft rejection, based on the affinity of surface receptors of lymphocytes for histocompatibility antigens. It was also shown that sensitisation to minor histocompatibility antigens in the Ag-B-compatible AS to Lewis combination led to fierce rejection of a subsequent renal allograft, which was not rejected by the nonsensitised recipient.", "contents": "Studies on the specific suppression of renal allograft rejection in presensitised rats. Theoretical and clinical implications. DA and Lewis rats were sensitised by two (DA times Lewis)F1 skin allografts 6 weeks apart and were given a (DA times Lewis)F1 renal allograft 4 weeks after the second skin allograft. Passive enhancement was tbsted in both strain combinations, the (DA times Lewis)F1 to DA model representing the weaker histocompatibility barrier as judged by renal allograft rejection. Passive enhancemnt was much less effective in the sensitised weaker model and was sometimes virtually ineffective in the sensitised stronger model. The powerful combination of antilymphocyte serum and enhancing serum could only delay but not prevent rejection in the latter model. Thus, the avoidance of sensitisation might be of critical importance for the clinical applicability of passive enhancement. Furthermore, it was shown that a sensitised cellular immune system is susceptible to specific immunosuppression, but the result depends on the strain combination used and whether the test graft is skin or kidney. A hypothesis is presented to explain the basis for the difference in strength of different histocompatibility barriers and also the differences between skin and kidney allograft rejection, based on the affinity of surface receptors of lymphocytes for histocompatibility antigens. It was also shown that sensitisation to minor histocompatibility antigens in the Ag-B-compatible AS to Lewis combination led to fierce rejection of a subsequent renal allograft, which was not rejected by the nonsensitised recipient."}
{"id": "PMID:1091038", "title": "Prolongation of allogeneic heart graft survival in the rat after implantation on portal vein.", "content": "In rats, heart allografts with venous drainage via the portal vein were performed and compared with similar grafts draining into the inferior vena cava. A new technique was developed using the complete thoracic descending aorta and ligation of all its branches. The descending thoracic aorta was anastomosed to the recipient's abdominal aorta below the left renal vein. The pulmonary artery was implanted on the portal vein in an end to side fashion. In two rat strain combinations the allograft survival time was significantly prolonged when compared to that of the control grafts with pulmonary-caval anastomosis. The presumed mechanism for this prolonged graft survival might be the sequestration or degradation of transplantation antigens in the liver during their first passage through this organ. In this way only a small amount of antigen reaches the systemic circulation.", "contents": "Prolongation of allogeneic heart graft survival in the rat after implantation on portal vein. In rats, heart allografts with venous drainage via the portal vein were performed and compared with similar grafts draining into the inferior vena cava. A new technique was developed using the complete thoracic descending aorta and ligation of all its branches. The descending thoracic aorta was anastomosed to the recipient's abdominal aorta below the left renal vein. The pulmonary artery was implanted on the portal vein in an end to side fashion. In two rat strain combinations the allograft survival time was significantly prolonged when compared to that of the control grafts with pulmonary-caval anastomosis. The presumed mechanism for this prolonged graft survival might be the sequestration or degradation of transplantation antigens in the liver during their first passage through this organ. In this way only a small amount of antigen reaches the systemic circulation."}
{"id": "PMID:1091039", "title": "Cardiac heterotransplantation. Morphological and immunohistological studies.", "content": "A vascularized heterograft model using outbred strains of animals was developed by transplanting mouse hearts heterotopically into rats. With this species desparity rapid but not immediate graft rejection was observed, with a predictably narrow range of graft survival times. Morphological and immunohistological studies showed early deposition of fibrinogen and vascular and myocardial inflammation without prominent or consistent localization of either IgG or C3. Later more extensive changes were observed, and deposition of IgG and C3 were more prominent in the grafts. Pretreatment of the recipient with cyclophosphamide alone or cyclophosphamide plus antigen prolonged graft survival; however, no statistically significant difference was noted between these groups. Morphological and immunohistological alterations preceded clinical rejection, and tissue injury appeared to be mediated by humoral and cellular immune mechanisms and by the coagulation system. This model is potentially useful for the study of heterotransplantation.", "contents": "Cardiac heterotransplantation. Morphological and immunohistological studies. A vascularized heterograft model using outbred strains of animals was developed by transplanting mouse hearts heterotopically into rats. With this species desparity rapid but not immediate graft rejection was observed, with a predictably narrow range of graft survival times. Morphological and immunohistological studies showed early deposition of fibrinogen and vascular and myocardial inflammation without prominent or consistent localization of either IgG or C3. Later more extensive changes were observed, and deposition of IgG and C3 were more prominent in the grafts. Pretreatment of the recipient with cyclophosphamide alone or cyclophosphamide plus antigen prolonged graft survival; however, no statistically significant difference was noted between these groups. Morphological and immunohistological alterations preceded clinical rejection, and tissue injury appeared to be mediated by humoral and cellular immune mechanisms and by the coagulation system. This model is potentially useful for the study of heterotransplantation."}
{"id": "PMID:1091040", "title": "Immunological enhancement of skin allografts in the rat. Role of vascular and lymphatic reconstitution.", "content": "Four skin graft models are created which vary in the rates of lymphatic and vascular reconstitution. Rat allografts (Brown Norway or (L times BN)F1 hybrid) transplanted to Lewis recipients by conventional (immediate lymphatic, delayed vascular), surgical anastomosis (immediate lymphatic, immediate vascular) or isolated anastomosis (delayed lymphatic, immediate vascular) techniques have similar mean survival times (7.8 days). Grafts placed on an isolated recipient pedicle (delayed lymphatic, delayed vascular) double the mean survival time to 15.6 days. Treatment with alloimmune serum, able to indefinitely prolong survival of similarly mismatched renal grafts, prolongs only isolated anastomosed grafts. In contrast, cyclophosphamide treatment prolongs survival of all groups. These results suggest, first, that both vascular and lymphatic routes of sensitization are equally effective and, second, that immunological enhancement requires either prompt vascular continuity or a persistent lack of lymphatic reconstitution.", "contents": "Immunological enhancement of skin allografts in the rat. Role of vascular and lymphatic reconstitution. Four skin graft models are created which vary in the rates of lymphatic and vascular reconstitution. Rat allografts (Brown Norway or (L times BN)F1 hybrid) transplanted to Lewis recipients by conventional (immediate lymphatic, delayed vascular), surgical anastomosis (immediate lymphatic, immediate vascular) or isolated anastomosis (delayed lymphatic, immediate vascular) techniques have similar mean survival times (7.8 days). Grafts placed on an isolated recipient pedicle (delayed lymphatic, delayed vascular) double the mean survival time to 15.6 days. Treatment with alloimmune serum, able to indefinitely prolong survival of similarly mismatched renal grafts, prolongs only isolated anastomosed grafts. In contrast, cyclophosphamide treatment prolongs survival of all groups. These results suggest, first, that both vascular and lymphatic routes of sensitization are equally effective and, second, that immunological enhancement requires either prompt vascular continuity or a persistent lack of lymphatic reconstitution."}
{"id": "PMID:1091041", "title": "A modified rosette inhibition test in renal allotransplantation.", "content": "The rosette inhibition test, with a modification of the technique which enables highly accurate marking of T lymphocytes, has been employed in the followup of 55 renal transplant patients. The minimal inhibitory concentration of antilymphocyte globulin (that is, that concentration of antilymphocyte globulin causing 25% inhibition of rosette formation) was higher than 1:16,000 in 63 (97%) of 65 separate determinations made during acute rejection episodes, and lower than 1:16,000 in 377 (92%) of 410 determinations after which no clinical evidence of rejection developed. The results presented in this paper indicate that this modified test is a useful tool either to predict the occurrence of or to confirm the diagnosis of rejection.", "contents": "A modified rosette inhibition test in renal allotransplantation. The rosette inhibition test, with a modification of the technique which enables highly accurate marking of T lymphocytes, has been employed in the followup of 55 renal transplant patients. The minimal inhibitory concentration of antilymphocyte globulin (that is, that concentration of antilymphocyte globulin causing 25% inhibition of rosette formation) was higher than 1:16,000 in 63 (97%) of 65 separate determinations made during acute rejection episodes, and lower than 1:16,000 in 377 (92%) of 410 determinations after which no clinical evidence of rejection developed. The results presented in this paper indicate that this modified test is a useful tool either to predict the occurrence of or to confirm the diagnosis of rejection."}
{"id": "PMID:1091043", "title": "Characteristics of histocompatibility barriers in congenic strains of mice. III. Passive enhancement of skin allografts in X-irradiated hosts.", "content": "Passive immunological enhancement of skin allografts was investigated in three donor-host combinations of congenic mice disparate at non-H-2 loci. Serum against the graft donor was derived from mice that had received donor strain lymphoid cells as neonates, and thereby were rendered specifically tolerant of a skin allograft. We refer to this serum as \"allograft-tolerant\" serum. Each strain combination was chosen to provide only two non-H-2 histoincompatibilities present in the donor and absent in the host. The differences are categorized as immunogenetically strong, moderate, or weak, on the basis of skin allograft survival times. With passively administered allograft-tolerant serum significantly prolonged graft survivals were noted for the weakest combination only. Combined treatment with sublethal X-irradiation and allograft-tolerant serum signigicantly prolonged graft survivals were noted for the weakest combination only. Combined treatment with sublethal X-irradiation and allograft-tolerant serum significantly prolonged graft survival in both the moderate and weak combinations, with the largest effect present in the weakest disparity. A hyperimmune alloantiserum (produced in adults) directed against the graft donor prolonged allograft survival in the strongest disparity when given in combination with irradiation. In this combination, graft survival time was increased in hosts exposed to X-ray alone, but joint treatment with X-ray and the alloantiserum gave the largest increment. In contrast, combined treatment with the serum and an antithymocyte alloantiserum did not affect graft survival times. Treatment with both radiation and antithymocyte serum did not prolong graft survival beyond that in mice given only X-radiation. Immunological enhancement with central inhibition is assumed as the mechanism underlying prolonged graft survival, and it is suggested that a population of thymus-derived \"killer\" cells, sensitive to X-irradiation, is required for normal graft rejection.", "contents": "Characteristics of histocompatibility barriers in congenic strains of mice. III. Passive enhancement of skin allografts in X-irradiated hosts. Passive immunological enhancement of skin allografts was investigated in three donor-host combinations of congenic mice disparate at non-H-2 loci. Serum against the graft donor was derived from mice that had received donor strain lymphoid cells as neonates, and thereby were rendered specifically tolerant of a skin allograft. We refer to this serum as \"allograft-tolerant\" serum. Each strain combination was chosen to provide only two non-H-2 histoincompatibilities present in the donor and absent in the host. The differences are categorized as immunogenetically strong, moderate, or weak, on the basis of skin allograft survival times. With passively administered allograft-tolerant serum significantly prolonged graft survivals were noted for the weakest combination only. Combined treatment with sublethal X-irradiation and allograft-tolerant serum signigicantly prolonged graft survivals were noted for the weakest combination only. Combined treatment with sublethal X-irradiation and allograft-tolerant serum significantly prolonged graft survival in both the moderate and weak combinations, with the largest effect present in the weakest disparity. A hyperimmune alloantiserum (produced in adults) directed against the graft donor prolonged allograft survival in the strongest disparity when given in combination with irradiation. In this combination, graft survival time was increased in hosts exposed to X-ray alone, but joint treatment with X-ray and the alloantiserum gave the largest increment. In contrast, combined treatment with the serum and an antithymocyte alloantiserum did not affect graft survival times. Treatment with both radiation and antithymocyte serum did not prolong graft survival beyond that in mice given only X-radiation. Immunological enhancement with central inhibition is assumed as the mechanism underlying prolonged graft survival, and it is suggested that a population of thymus-derived \"killer\" cells, sensitive to X-irradiation, is required for normal graft rejection."}
{"id": "PMID:1091045", "title": "Corticosteroids in organ preservation.", "content": "1. Glucocorticoids have been empirically employed during perfusion preservation of transplantable organs. 2. Use of corticosteroids in organ preservation is based on their pharmocologic binding of released lysosomal enzymes, as membrane stabilizers, peripheral vasodilators, and inhibitors of the inflammatory response. 3. The incidence post-transplant of ATN in cadaveric kidney preservation by pulsa-tile perfusion has been reduced from 57% to 18% when the donor was protected with large doses of M-P. 4. There is sufficient clinical and laboratory evidence to substantiate the use of large doses of M-P given to the prospective donor as a protective agent against ischemic injury in organ recovery.", "contents": "Corticosteroids in organ preservation. 1. Glucocorticoids have been empirically employed during perfusion preservation of transplantable organs. 2. Use of corticosteroids in organ preservation is based on their pharmocologic binding of released lysosomal enzymes, as membrane stabilizers, peripheral vasodilators, and inhibitors of the inflammatory response. 3. The incidence post-transplant of ATN in cadaveric kidney preservation by pulsa-tile perfusion has been reduced from 57% to 18% when the donor was protected with large doses of M-P. 4. There is sufficient clinical and laboratory evidence to substantiate the use of large doses of M-P given to the prospective donor as a protective agent against ischemic injury in organ recovery."}
{"id": "PMID:1091052", "title": "Corticosteroids in clinical and experimental lung transplantation.", "content": "Lung allograft rejection can usually be diagnosed by the appearance of infiltrates on plain chest roentgenograms when these are interpreted in the light of other clinical and bacteriologic information. Large pulsed intravenous doses of methylprednisolone were usually effective in reversing lung allograft rejection that occurred in immunosuppressed dogs. In 10 of 15 animals the presence of moderate to severe rejection and its effective reversal with treatment were documented with roentgenograms and histologic sections. This ability to reverse the manifestations of lung allograft rejection, when they occur, has helped in the management of human lung allograft recipients.", "contents": "Corticosteroids in clinical and experimental lung transplantation. Lung allograft rejection can usually be diagnosed by the appearance of infiltrates on plain chest roentgenograms when these are interpreted in the light of other clinical and bacteriologic information. Large pulsed intravenous doses of methylprednisolone were usually effective in reversing lung allograft rejection that occurred in immunosuppressed dogs. In 10 of 15 animals the presence of moderate to severe rejection and its effective reversal with treatment were documented with roentgenograms and histologic sections. This ability to reverse the manifestations of lung allograft rejection, when they occur, has helped in the management of human lung allograft recipients."}
{"id": "PMID:1091053", "title": "[Apparatus for automatic recording of the intensity of ultraviolet fluorescence from individual cells].", "content": "The device enables to examine about 40 000--60 000 cells per min. The results are presented by the distribution curves of the ultraviolet fluorescence intensity.", "contents": "[Apparatus for automatic recording of the intensity of ultraviolet fluorescence from individual cells]. The device enables to examine about 40 000--60 000 cells per min. The results are presented by the distribution curves of the ultraviolet fluorescence intensity."}
{"id": "PMID:1091054", "title": "Case study using simultaneous bladder pressure and urine loss measurements.", "content": "Using an \"electronic nappy\" of improved design and a miniature pressure manometer of the strain-gauge type mounted at the tip of a catheter, the case of a patient with spina bifida and a large lipoma has been investigated. Some retention exists, but areas on the lipoma surface can be touched lightly, causing an increase in bladder pressure with subsequent voiding as measured by the nappy. Investigations of the same area by electrical stimulation produced rises in bladder pressure sustained for only a few seconds, as in the case of manual stimulation. Local anaesthetic in the \"trigger\" area prevented bladder pressure changes and urine loss, but triggering returned as the anaesthetic wore off.", "contents": "Case study using simultaneous bladder pressure and urine loss measurements. Using an \"electronic nappy\" of improved design and a miniature pressure manometer of the strain-gauge type mounted at the tip of a catheter, the case of a patient with spina bifida and a large lipoma has been investigated. Some retention exists, but areas on the lipoma surface can be touched lightly, causing an increase in bladder pressure with subsequent voiding as measured by the nappy. Investigations of the same area by electrical stimulation produced rises in bladder pressure sustained for only a few seconds, as in the case of manual stimulation. Local anaesthetic in the \"trigger\" area prevented bladder pressure changes and urine loss, but triggering returned as the anaesthetic wore off."}
{"id": "PMID:1091056", "title": "[Congenital bulbular urethral constrictures. Pathogenesis and treatment of diseases of the bladder neck and urogenital border regions(author's transl)].", "content": "This paper is a survey of the literature concerning diseases of the bladder neck, posterior urethra and the urogenital border region. Urethrographic, uroflowmetric and urethrocystoscopic examinations of more than 200 patients ranging in age from one day to over 70 years demonstrated a clear-cut bulbular urethral constricture in this broad-spectrum disease picture. Its a congenital appearance is explained embryologically. A case analysis allows for classification based on the functional value of the urethral constricture. Diseases of as yet unexplained etiology such as Marion's disease, bladder neck sclerosis, urethral valves and chronic prostatitis-prostatopathy are clarified, based on the demonstrated urodynamics.", "contents": "[Congenital bulbular urethral constrictures. Pathogenesis and treatment of diseases of the bladder neck and urogenital border regions(author's transl)]. This paper is a survey of the literature concerning diseases of the bladder neck, posterior urethra and the urogenital border region. Urethrographic, uroflowmetric and urethrocystoscopic examinations of more than 200 patients ranging in age from one day to over 70 years demonstrated a clear-cut bulbular urethral constricture in this broad-spectrum disease picture. Its a congenital appearance is explained embryologically. A case analysis allows for classification based on the functional value of the urethral constricture. Diseases of as yet unexplained etiology such as Marion's disease, bladder neck sclerosis, urethral valves and chronic prostatitis-prostatopathy are clarified, based on the demonstrated urodynamics."}
{"id": "PMID:1091063", "title": "Late ureteropelvic necrosis after transplantation.", "content": "Total ureteropelvic necrosis of the transplanted kidney occurred more than one month after transplantation in 5 of 575 consecutive renal transplants performed at the University of Minnesota Hospital since 1963. Necrosis became evident long after normal renal function had been established. Histologic signs of rejection were minimal, but perinephric or periureteral hematomas were found in 3 of 5 patients: post-transplant acute tubular necorsis requiring hemodialysis occurred in all. The pathogenesis of this complication probably involves (1) a primary deficit of blood supply from the renal vessels to the pelvis and ureter, (2) a failure to develop a new ureteral blood supply because of surrounding hematoma, (3) early swelling of the ischemic ureter resulting in oliguria interpreted as acute tubular necrosis, (4) resolution of edema resulting in diuresis, and (5) late patchy ureteral necrosis and fistula formation due to ureteral ischemia.", "contents": "Late ureteropelvic necrosis after transplantation. Total ureteropelvic necrosis of the transplanted kidney occurred more than one month after transplantation in 5 of 575 consecutive renal transplants performed at the University of Minnesota Hospital since 1963. Necrosis became evident long after normal renal function had been established. Histologic signs of rejection were minimal, but perinephric or periureteral hematomas were found in 3 of 5 patients: post-transplant acute tubular necorsis requiring hemodialysis occurred in all. The pathogenesis of this complication probably involves (1) a primary deficit of blood supply from the renal vessels to the pelvis and ureter, (2) a failure to develop a new ureteral blood supply because of surrounding hematoma, (3) early swelling of the ischemic ureter resulting in oliguria interpreted as acute tubular necrosis, (4) resolution of edema resulting in diuresis, and (5) late patchy ureteral necrosis and fistula formation due to ureteral ischemia."}
{"id": "PMID:1091065", "title": "Use of procine zenografts in treatment of Fournier's gangrene.", "content": "The use of procine xenografts as a biologic dressing has become increasingly popular, particularly in cases of extensive tissue loss, as in severe thermal injuries. It not only minimizes protein and fluid loss, acts as a barrier to infection, but also prevents conversion of second-degree burns through its ability to control existing infection. In this case, it was used successfully to treat Fournier's gangrene. We believe it significantly eased and shortened the patient's hospital stay and enabled us to apply autogenous split thickness skin grafts sooner than with conventional modes of wound care.", "contents": "Use of procine zenografts in treatment of Fournier's gangrene. The use of procine xenografts as a biologic dressing has become increasingly popular, particularly in cases of extensive tissue loss, as in severe thermal injuries. It not only minimizes protein and fluid loss, acts as a barrier to infection, but also prevents conversion of second-degree burns through its ability to control existing infection. In this case, it was used successfully to treat Fournier's gangrene. We believe it significantly eased and shortened the patient's hospital stay and enabled us to apply autogenous split thickness skin grafts sooner than with conventional modes of wound care."}
{"id": "PMID:1091067", "title": "Complete duplication of urethra.", "content": "Complete duplication of the urethra with a single bladder is an exceptional finding. The anomalous urethral canal originates from the bladder separately, runs parallel and usually dorsal to the normally situated urethra, and opens on the dorsum of the penis. To our knowledge there are 41 reported cases of this anomaly. We present 2 cases of complete urethral duplication originating from a single bladder, one in a male patient and the other in a female. It is concluded that conservative therapy is the treatment of choice, and that surgery should be reserved for incontinent patients only. The complications of surgery have been emphasized.", "contents": "Complete duplication of urethra. Complete duplication of the urethra with a single bladder is an exceptional finding. The anomalous urethral canal originates from the bladder separately, runs parallel and usually dorsal to the normally situated urethra, and opens on the dorsum of the penis. To our knowledge there are 41 reported cases of this anomaly. We present 2 cases of complete urethral duplication originating from a single bladder, one in a male patient and the other in a female. It is concluded that conservative therapy is the treatment of choice, and that surgery should be reserved for incontinent patients only. The complications of surgery have been emphasized."}
{"id": "PMID:1091069", "title": "[Comprative evaluation of the immediate results of gastrectomy with different types of esophago-intestinal anastomosis].", "content": "Using the experience with 207 gastrectomies the author presents an analysis of a dependence of incompetency of enteroesophageal anastomosis sutures on the operative approach, extent of surgery, method of enteroesophagostomy, number of suture rows. The author's data evidence that incompetency of anastomosis sutures in gastrectomy is the principal cause of mortality in the postoperative period. The mortality due to suture incompetency while using a thoracoabdominal access is considerably higher than in transabdominal approach. The Sapozhnikov-Judin technic yields the least per cent of anastomotic suture incompetency (2%), whereas the Gilyarovich method--the highest (18.7%). The use of three-row sutures in formation of the anastomosis decreases twice the percent of suture incompetency. Extension of gastrectomy to a combined one does not increase the percentage of suture incompetency. Constant improvement of the Sapozhnikov-Judin method might permit to reduce to a minimum the anastomosis suture incompetency in gastrectomy.", "contents": "[Comprative evaluation of the immediate results of gastrectomy with different types of esophago-intestinal anastomosis]. Using the experience with 207 gastrectomies the author presents an analysis of a dependence of incompetency of enteroesophageal anastomosis sutures on the operative approach, extent of surgery, method of enteroesophagostomy, number of suture rows. The author's data evidence that incompetency of anastomosis sutures in gastrectomy is the principal cause of mortality in the postoperative period. The mortality due to suture incompetency while using a thoracoabdominal access is considerably higher than in transabdominal approach. The Sapozhnikov-Judin technic yields the least per cent of anastomotic suture incompetency (2%), whereas the Gilyarovich method--the highest (18.7%). The use of three-row sutures in formation of the anastomosis decreases twice the percent of suture incompetency. Extension of gastrectomy to a combined one does not increase the percentage of suture incompetency. Constant improvement of the Sapozhnikov-Judin method might permit to reduce to a minimum the anastomosis suture incompetency in gastrectomy."}
{"id": "PMID:1091074", "title": "[Selection of pancreato-intestinal anastomosis in pancreatoduodenal resection].", "content": "41 pancreatoduodenal resections with 11 lethal issues (26.8 plus or minus 7%) were analysed. In 21 cases an end-to-end pancreatointestinal anastomosis was employed, in 12 cases an end-to-side one with peritonization of a suture line with the jejunal wall after the A. A. Shalimov technic, but in 8 cases- the technic of pancreatojejunostomy elaborated by the author, an end-to-end side type enveloping the anastomosis with a cecal segment of the jejunum. This method of peritonization does not impede passage of the intestinal content, thus rendering unnecessary the production of enteroenterostomy. Suture divergency of the end-to-end pancreatointestinal anastomosis was noted postoperatively in 6 of 21 patients (28.6 plus or minus 10%). Whereas, among 20 pancreatojejunostomies with peritonization of sutures by the jejunal wall after the A. A. Shalimov technic or in the author's modification this complication was noted only in 2 cases (10 plus or minus 7%).", "contents": "[Selection of pancreato-intestinal anastomosis in pancreatoduodenal resection]. 41 pancreatoduodenal resections with 11 lethal issues (26.8 plus or minus 7%) were analysed. In 21 cases an end-to-end pancreatointestinal anastomosis was employed, in 12 cases an end-to-side one with peritonization of a suture line with the jejunal wall after the A. A. Shalimov technic, but in 8 cases- the technic of pancreatojejunostomy elaborated by the author, an end-to-end side type enveloping the anastomosis with a cecal segment of the jejunum. This method of peritonization does not impede passage of the intestinal content, thus rendering unnecessary the production of enteroenterostomy. Suture divergency of the end-to-end pancreatointestinal anastomosis was noted postoperatively in 6 of 21 patients (28.6 plus or minus 10%). Whereas, among 20 pancreatojejunostomies with peritonization of sutures by the jejunal wall after the A. A. Shalimov technic or in the author's modification this complication was noted only in 2 cases (10 plus or minus 7%)."}
{"id": "PMID:1091075", "title": "[Results of treatment of primary bone neoplasms of the sacrococcygeal region].", "content": "In the clinic 53 patients with primary bone tumors of the sacrococcygeal region and sacroiliac junctions were observed. Surgical methods of treatment were used in 33, combined--in 7, radiation therapy--in 10, drug therapy--in 3. Recurrences developed in 7 cases, metastases were revealed in 14. The results of treatment in patients with neoplasms in this region were studied. Twenty five patients died from generalization of the process, metastases, intestinal obstruction and uremia. The remaining 28 patients have been followed up within the terms from 1 to 5 years and longer.", "contents": "[Results of treatment of primary bone neoplasms of the sacrococcygeal region]. In the clinic 53 patients with primary bone tumors of the sacrococcygeal region and sacroiliac junctions were observed. Surgical methods of treatment were used in 33, combined--in 7, radiation therapy--in 10, drug therapy--in 3. Recurrences developed in 7 cases, metastases were revealed in 14. The results of treatment in patients with neoplasms in this region were studied. Twenty five patients died from generalization of the process, metastases, intestinal obstruction and uremia. The remaining 28 patients have been followed up within the terms from 1 to 5 years and longer."}
{"id": "PMID:1091076", "title": "[Results of bone homoplasty in ununited fractures, pseudarthroses and defects of the long tumular bones].", "content": "In the article, observations on application of bone homoplasty in 99 patients with unhealing fractures, pseudarthrosis and defects of long tumular bones are described. Homoplasty per se was performed in 22 cases, in combination with autoplasty--in 3, with metallic osteosynthesis--in 74. Tubular homografts were used in 14 cases, grafts in a kind of cortical plates--in 85. Postoperatively, the patients were followed within the terms up to 5 years. In 88 cases the results of surgery were satisfactory, in 11--poor. An analysis of the material concerned have shown that issues of bone homoplasty are greatly effected by the character of the graft, surgical technic and the state of a recipient bed.", "contents": "[Results of bone homoplasty in ununited fractures, pseudarthroses and defects of the long tumular bones]. In the article, observations on application of bone homoplasty in 99 patients with unhealing fractures, pseudarthrosis and defects of long tumular bones are described. Homoplasty per se was performed in 22 cases, in combination with autoplasty--in 3, with metallic osteosynthesis--in 74. Tubular homografts were used in 14 cases, grafts in a kind of cortical plates--in 85. Postoperatively, the patients were followed within the terms up to 5 years. In 88 cases the results of surgery were satisfactory, in 11--poor. An analysis of the material concerned have shown that issues of bone homoplasty are greatly effected by the character of the graft, surgical technic and the state of a recipient bed."}
{"id": "PMID:1091081", "title": "Factors influencing occurrence of colibacillosis in calves.", "content": "This investigation describes some of the husbandry factors influencing the occurrence of colibacillosis in calves. Diarrhoea and mortality were usually associated with an increase in the proportion of \"pathogenic\" to total E coli to about 50 per cent in the faeces although there were occasions when the proportion of \"pathogenic\" E coli increased but no disease occurred. These increases often followed changes of diet which appeared to be more important than environmental conditions. The use of uncontaminated houses to break the cycle of infection appeared to have the greatest influence on the disease pattern.", "contents": "Factors influencing occurrence of colibacillosis in calves. This investigation describes some of the husbandry factors influencing the occurrence of colibacillosis in calves. Diarrhoea and mortality were usually associated with an increase in the proportion of \"pathogenic\" to total E coli to about 50 per cent in the faeces although there were occasions when the proportion of \"pathogenic\" E coli increased but no disease occurred. These increases often followed changes of diet which appeared to be more important than environmental conditions. The use of uncontaminated houses to break the cycle of infection appeared to have the greatest influence on the disease pattern."}
{"id": "PMID:1091082", "title": "Chromosomes of cattle: present clinical status and promise.", "content": "Current information on cytogenetics in veterinary medicine is presented as a resume for the veterinarian in cattle practice; previous reviews of the subject are recorded. The literature depicting the recognised chromosome abnormalities in cattle is breifly reviewed. It is suggested that chromosome screening already has a role to play in the economics of cattle production.", "contents": "Chromosomes of cattle: present clinical status and promise. Current information on cytogenetics in veterinary medicine is presented as a resume for the veterinarian in cattle practice; previous reviews of the subject are recorded. The literature depicting the recognised chromosome abnormalities in cattle is breifly reviewed. It is suggested that chromosome screening already has a role to play in the economics of cattle production."}
{"id": "PMID:1091089", "title": "[Specific binding of progesterone to the proteins of human endometrial cells].", "content": "A specific complex of protein-receptor with hormone, which had a sedimentation coefficient 3.8S, was formed in cell nuclei on incubation of endometrial tissue from pregnant woman with 3H-progestrone at 37 degrees C in vitro. In cell cytoplasm of endometrium the progesterone-protein complex was observed which sedimented at 6.8S and 4.2S. Progesterone was accumulated mainly in nuclei; lower concentrations of the hormone were found in cell cytoplasm of endometrium. Fractions of mitochondria, microsomes and ribosomes also bound the hormone but less distinct as compared with the nuclei. The formation of cytoplasmic complex of progesterone with protein-receptor occurred on incubation of endometrial cytoplasmic fraction with 3h-progesterone at 4 degrees C.", "contents": "[Specific binding of progesterone to the proteins of human endometrial cells]. A specific complex of protein-receptor with hormone, which had a sedimentation coefficient 3.8S, was formed in cell nuclei on incubation of endometrial tissue from pregnant woman with 3H-progestrone at 37 degrees C in vitro. In cell cytoplasm of endometrium the progesterone-protein complex was observed which sedimented at 6.8S and 4.2S. Progesterone was accumulated mainly in nuclei; lower concentrations of the hormone were found in cell cytoplasm of endometrium. Fractions of mitochondria, microsomes and ribosomes also bound the hormone but less distinct as compared with the nuclei. The formation of cytoplasmic complex of progesterone with protein-receptor occurred on incubation of endometrial cytoplasmic fraction with 3h-progesterone at 4 degrees C."}
{"id": "PMID:1091090", "title": "[Metabolic stability of the protein-synthesizing system of the myocardium in compensatory cardiac hyperfunction in actinomycin D block of RNA synthesis].", "content": "Under conditions of complete actinomycin block in rat heart muscle an inhibition of protein synthesis and dissociation of myocardial polyribosome structures were observed (half-life was equal to 6.5 hrs). In compensatory hyperfunction of heart muscle, caused by stenosis of aorta, the inhibitory effect of actinomycin D on the protein synthesis was manifested earlier than in control and was exhibited more distinctly. In myocardium under compensatory hyperfunction of heart muscle the protein synthesis was decreased by 50% within 4 hrs after administration of actinomycin into animals and it was completely inhibited within 10 hrs after the antibiotic administration. The alteration in stability of polyritobosomes under compensatory hyperfunction of heart muscle was specific for myocardium and was not observed in liver tissue.", "contents": "[Metabolic stability of the protein-synthesizing system of the myocardium in compensatory cardiac hyperfunction in actinomycin D block of RNA synthesis]. Under conditions of complete actinomycin block in rat heart muscle an inhibition of protein synthesis and dissociation of myocardial polyribosome structures were observed (half-life was equal to 6.5 hrs). In compensatory hyperfunction of heart muscle, caused by stenosis of aorta, the inhibitory effect of actinomycin D on the protein synthesis was manifested earlier than in control and was exhibited more distinctly. In myocardium under compensatory hyperfunction of heart muscle the protein synthesis was decreased by 50% within 4 hrs after administration of actinomycin into animals and it was completely inhibited within 10 hrs after the antibiotic administration. The alteration in stability of polyritobosomes under compensatory hyperfunction of heart muscle was specific for myocardium and was not observed in liver tissue."}
{"id": "PMID:1091092", "title": "[Noncoagulative catabolism of fibrinogen].", "content": "In catabolism of fibrinogen a function of Cupfer endothelial cells isolated from dog and rabbit liver tissue, was studied. In lysosomal-mitochondrial fraction of Cupfer cells and activator of plasminogen was found. Proteolytic and esterase (hydrolysis of benzoyll-agronine methyl ester) activities were the most typical for lysosomal-mitochondril and supernatant fractions of Cupfer cells. The enzymatic hydrolysis of fibrinogen was not observed in cytoplasmic matrix. The data obtained suggest that under the physiological conditions non-coagulational catabolism of fibrinogen occurred mainly intracellularly.", "contents": "[Noncoagulative catabolism of fibrinogen]. In catabolism of fibrinogen a function of Cupfer endothelial cells isolated from dog and rabbit liver tissue, was studied. In lysosomal-mitochondrial fraction of Cupfer cells and activator of plasminogen was found. Proteolytic and esterase (hydrolysis of benzoyll-agronine methyl ester) activities were the most typical for lysosomal-mitochondril and supernatant fractions of Cupfer cells. The enzymatic hydrolysis of fibrinogen was not observed in cytoplasmic matrix. The data obtained suggest that under the physiological conditions non-coagulational catabolism of fibrinogen occurred mainly intracellularly."}
{"id": "PMID:1091093", "title": "[Blood proteolytic enzyme inhibitors and their clinical study].", "content": "Data on biochemistry, physiological significance and clinical investigations of two main inhibitors of proteinases in human blood serum (alpha 1-antitrypsin and alpha 2-macroglobulin) are reviewed. Physico-chemical properties of the inhibitors, mechanisms of their interaction with proteinases and methods for determination are discussed. Modification of the specificity of some proteinases by alpha 2-macroglobulin, which probably plays a significant role in the regulation of enzymatic systems of proteolysis in blood, is considered. The use of the inhibitors of proteolysis for diagnostics of pathological processes is discussed. Data on the alpha 1-antitrypsin deficiency and its relation to the development of degenerative diseases in lungs are reviewed.", "contents": "[Blood proteolytic enzyme inhibitors and their clinical study]. Data on biochemistry, physiological significance and clinical investigations of two main inhibitors of proteinases in human blood serum (alpha 1-antitrypsin and alpha 2-macroglobulin) are reviewed. Physico-chemical properties of the inhibitors, mechanisms of their interaction with proteinases and methods for determination are discussed. Modification of the specificity of some proteinases by alpha 2-macroglobulin, which probably plays a significant role in the regulation of enzymatic systems of proteolysis in blood, is considered. The use of the inhibitors of proteolysis for diagnostics of pathological processes is discussed. Data on the alpha 1-antitrypsin deficiency and its relation to the development of degenerative diseases in lungs are reviewed."}
{"id": "PMID:1091100", "title": "[Napkin thrush clinical picture, mycology, histology, therapy (author's transl)].", "content": "A well-defined, characteristic disease has been observed in babies at the Department of Dermatology, Innsbruck University, over the past 7 years. On account of the localisation and probable pathogenesis of the condition the term \"Windelsoor\" (napkin thrush) has been coined by us for it. The clinical picture, the mycological and histological findings, the therapeutic results and the follow up are reported and the aetiology and pathogenesis of this disease discussed.", "contents": "[Napkin thrush clinical picture, mycology, histology, therapy (author's transl)]. A well-defined, characteristic disease has been observed in babies at the Department of Dermatology, Innsbruck University, over the past 7 years. On account of the localisation and probable pathogenesis of the condition the term \"Windelsoor\" (napkin thrush) has been coined by us for it. The clinical picture, the mycological and histological findings, the therapeutic results and the follow up are reported and the aetiology and pathogenesis of this disease discussed."}
{"id": "PMID:1091116", "title": "The measurement of hypothalamic releasing hormone content and activity in the unanaesthetized rat by means of carotid artery cannulation.", "content": "To gain insights into quantitative changes in hypothalamic substances which affect the function of the anterior hypophyseal lobe, we developed a technique for carotid cannulation allowing sequential measurements of plasma levels of an affected hormone after injection of such a factor. The principle involves the use of a previously cannulated rat in an unanaesthetized unstressed condition. The hypothalamic extract or LH-RH injected into the cannulas of such animals leads to rapid peaks of LH in peripheral blood removed from the same cannulas within 6 to 8 min, followed by a slow decrease. At least 50% of the peak value is usually found within 3 min. The technique yields quantitatively reproducible results, and appears suitable for measuring relative potencies of test extracts against pure standards and, within as yet undetermined limits, for kinetic studies.", "contents": "The measurement of hypothalamic releasing hormone content and activity in the unanaesthetized rat by means of carotid artery cannulation. To gain insights into quantitative changes in hypothalamic substances which affect the function of the anterior hypophyseal lobe, we developed a technique for carotid cannulation allowing sequential measurements of plasma levels of an affected hormone after injection of such a factor. The principle involves the use of a previously cannulated rat in an unanaesthetized unstressed condition. The hypothalamic extract or LH-RH injected into the cannulas of such animals leads to rapid peaks of LH in peripheral blood removed from the same cannulas within 6 to 8 min, followed by a slow decrease. At least 50% of the peak value is usually found within 3 min. The technique yields quantitatively reproducible results, and appears suitable for measuring relative potencies of test extracts against pure standards and, within as yet undetermined limits, for kinetic studies."}
{"id": "PMID:1091117", "title": "Measurement of rat LH with a double-antibody solid-phase radioimmunoassay: effect of LH-RH and of testosterone oenanthate in castrated animals.", "content": "A double-antibody solid-phase radioimmunoassay for the measurement of rat LH was described. The use of a solid-phase method for the second incubation resulted in a considerable saving of time as compared to the conventional double-antibody method. The sensitivity and accuracy of the method were not affected by this modification. It was found that there was a cross-reaction between the NIAMD-anti-rat-LH-serum-1 used and NIAMD-rat-FSH-RP-1. This indicates that the test system does not specifically measure rat LH but gonadotrophic activity. The RIA was tested under different experimental conditions. In male rats, the effect of castration and of subsequent treatment with testosterone oenanthate upon serum LH, and upon the weight of the seminal vesicles, the ventral prostate, and the levator ani, was examined. Serum LH rose rapidly after castration and reached 2 to 4 weeks after orchidectomy a maximum (8-9 fold increase). Subsequent treatment with testosterone oenanthate caused a rapid decrease of serum LH. The accessory sex organs showed reciprocal changes of weight. In oophorectomized rats whose pituitaries had been blocked with oestradiol monobenzoate and progesterone, a linear log-dose response of plasma LH was found after administration of various doses of synthetic LH-RH.", "contents": "Measurement of rat LH with a double-antibody solid-phase radioimmunoassay: effect of LH-RH and of testosterone oenanthate in castrated animals. A double-antibody solid-phase radioimmunoassay for the measurement of rat LH was described. The use of a solid-phase method for the second incubation resulted in a considerable saving of time as compared to the conventional double-antibody method. The sensitivity and accuracy of the method were not affected by this modification. It was found that there was a cross-reaction between the NIAMD-anti-rat-LH-serum-1 used and NIAMD-rat-FSH-RP-1. This indicates that the test system does not specifically measure rat LH but gonadotrophic activity. The RIA was tested under different experimental conditions. In male rats, the effect of castration and of subsequent treatment with testosterone oenanthate upon serum LH, and upon the weight of the seminal vesicles, the ventral prostate, and the levator ani, was examined. Serum LH rose rapidly after castration and reached 2 to 4 weeks after orchidectomy a maximum (8-9 fold increase). Subsequent treatment with testosterone oenanthate caused a rapid decrease of serum LH. The accessory sex organs showed reciprocal changes of weight. In oophorectomized rats whose pituitaries had been blocked with oestradiol monobenzoate and progesterone, a linear log-dose response of plasma LH was found after administration of various doses of synthetic LH-RH."}
{"id": "PMID:1091118", "title": "Studies on the inhibitory effect of somatostatin on glucose induced insulin release in the isolated perfused rat pancreas.", "content": "Somatostatin, the recently discovered growth hormone release-inhibiting hormone in the hypothalamus, also inhibited glucose induced insulin release from the isolated perfused rat pancreas. The lowest effective dose of somatostatin was 1 ng/ml of perfusate. By increasing the dose to 100 ng/ml an almost complete inhibition of basal as well as stimulated insulin release was obtained. The inhibition of glucose stimulated insulin release mediated by somatostatin might be of competitive nature since somatostatin seemed to dislocate the glucose-insulin dose-response curve to the right.", "contents": "Studies on the inhibitory effect of somatostatin on glucose induced insulin release in the isolated perfused rat pancreas. Somatostatin, the recently discovered growth hormone release-inhibiting hormone in the hypothalamus, also inhibited glucose induced insulin release from the isolated perfused rat pancreas. The lowest effective dose of somatostatin was 1 ng/ml of perfusate. By increasing the dose to 100 ng/ml an almost complete inhibition of basal as well as stimulated insulin release was obtained. The inhibition of glucose stimulated insulin release mediated by somatostatin might be of competitive nature since somatostatin seemed to dislocate the glucose-insulin dose-response curve to the right."}
{"id": "PMID:1091119", "title": "Studies on the mechanism of somatostatin action on insulin release in man. I. Effect of blockade of alpha-adrenergic receptors.", "content": "The present studies have demonstrated that the growth hormone releaseinhibiting factor of the hypothalamus, somatostatin, exerts a prominent inhibiting action on glucose induced insulin release in man. This inhibition of insulin release could not be reversed by infusion of the alpha-adrenergic blocking agent, phentolamine. Our findings therefore suggest that the inhibiting effect of somatostatin on glucose induced insulin release is not mediated by the alpha-adrenergic receptors.", "contents": "Studies on the mechanism of somatostatin action on insulin release in man. I. Effect of blockade of alpha-adrenergic receptors. The present studies have demonstrated that the growth hormone releaseinhibiting factor of the hypothalamus, somatostatin, exerts a prominent inhibiting action on glucose induced insulin release in man. This inhibition of insulin release could not be reversed by infusion of the alpha-adrenergic blocking agent, phentolamine. Our findings therefore suggest that the inhibiting effect of somatostatin on glucose induced insulin release is not mediated by the alpha-adrenergic receptors."}
{"id": "PMID:1091120", "title": "Insulin components released by the normal and subtotal degranulated perifused islets of Langerhans of the rat.", "content": "The insulin components released from normal and tolbutamide treated islets of the rat were studied in a perifusion system. The treated islets showed diminished quantities of insulin secreted as compared to the controls and an increase in the proportions of \"big insulin\" secreted during the second phase of this release. On the other hand, the main component of insulin released during the first phase was \"little insulin\". These studies confirm that the subtotally degranulated islets are less efficient than the controls in producing insulin release.", "contents": "Insulin components released by the normal and subtotal degranulated perifused islets of Langerhans of the rat. The insulin components released from normal and tolbutamide treated islets of the rat were studied in a perifusion system. The treated islets showed diminished quantities of insulin secreted as compared to the controls and an increase in the proportions of \"big insulin\" secreted during the second phase of this release. On the other hand, the main component of insulin released during the first phase was \"little insulin\". These studies confirm that the subtotally degranulated islets are less efficient than the controls in producing insulin release."}
{"id": "PMID:1091122", "title": "Clinical and pharmacological evaluation of the effects of piribedil in patients with parkinsonism.", "content": "Nine patients with Parkinsonism were studied before and after treatment with piribedil, a dopamine-receptor stimulator. Piribedil appeared to have a slight anti-Parkinsonism effect upon bradykinesia, and possibly upon tremor, but did not improve rigidity. The chief toxic effects were drowsiness and confusion, and two patients experienced nausea. Changes in homovanillic acid in the cerebrospinal fluid indicated that the drug reduced the turnover of endogenous dopamine. In spite of this definite neuropharmacological action, no clear-cut associated clinical benefit was demonstrated. The significance of these findings is discussed.", "contents": "Clinical and pharmacological evaluation of the effects of piribedil in patients with parkinsonism. Nine patients with Parkinsonism were studied before and after treatment with piribedil, a dopamine-receptor stimulator. Piribedil appeared to have a slight anti-Parkinsonism effect upon bradykinesia, and possibly upon tremor, but did not improve rigidity. The chief toxic effects were drowsiness and confusion, and two patients experienced nausea. Changes in homovanillic acid in the cerebrospinal fluid indicated that the drug reduced the turnover of endogenous dopamine. In spite of this definite neuropharmacological action, no clear-cut associated clinical benefit was demonstrated. The significance of these findings is discussed."}
{"id": "PMID:1091123", "title": "Plasma and erythrocyte magnesium levels in patients with primary affective disorder during chronic lithium treatment.", "content": "Plasma and erythrocyte magnesium concentrations were measured in 79 clinic outpatients who had histories of bipolar or unipolar primary affective disorder and who were being chronically treated with lithium carbonate (60 patients) or placebo (19 patients). Although slight differences in the mean concentration of magnesium in plasma and erythrocytes were noted with lithium treatment, diagnosis, and sex, the differences overall failed to achieve statistical significance. In contrast to prior reports demonstrating increases in plasma magnesium during acute lithium carbonate treatment of affectively ill patients, these data suggest that the pre-lithium steady state is achieved during chronic lithium treatment.", "contents": "Plasma and erythrocyte magnesium levels in patients with primary affective disorder during chronic lithium treatment. Plasma and erythrocyte magnesium concentrations were measured in 79 clinic outpatients who had histories of bipolar or unipolar primary affective disorder and who were being chronically treated with lithium carbonate (60 patients) or placebo (19 patients). Although slight differences in the mean concentration of magnesium in plasma and erythrocytes were noted with lithium treatment, diagnosis, and sex, the differences overall failed to achieve statistical significance. In contrast to prior reports demonstrating increases in plasma magnesium during acute lithium carbonate treatment of affectively ill patients, these data suggest that the pre-lithium steady state is achieved during chronic lithium treatment."}
{"id": "PMID:1091124", "title": "A comparative trail of fluphenazine decanoate and flupenthixol decanoate.", "content": "A double-blind cross-over study is reported which compares the antipsychotic properties and the side effects of depot flupenthixol with fluphenazine decanoate in chronic schizophrenic inpatients. Special emphasis was laid on examining changes in the target symptoms of apathy/anergia and depression in which flupenthixol has been claimed to be particulary effective. No significant differences were found between treatments on schizophrenic symptoms, or as regards the extrapyramidal side effects produced by equipotent doses of the two drugs. Reasons for the essentially negative results are discussed against a background review of earlier optimistic studies of flupenthixol.", "contents": "A comparative trail of fluphenazine decanoate and flupenthixol decanoate. A double-blind cross-over study is reported which compares the antipsychotic properties and the side effects of depot flupenthixol with fluphenazine decanoate in chronic schizophrenic inpatients. Special emphasis was laid on examining changes in the target symptoms of apathy/anergia and depression in which flupenthixol has been claimed to be particulary effective. No significant differences were found between treatments on schizophrenic symptoms, or as regards the extrapyramidal side effects produced by equipotent doses of the two drugs. Reasons for the essentially negative results are discussed against a background review of earlier optimistic studies of flupenthixol."}
{"id": "PMID:1091125", "title": "Age-related changes of cells involved in immune responses.", "content": "The interaction between T- and B-lymphocytes in the immune response to sheep reythrocytes was revealed by estimating the direct plaque-forming cells in mice CMA of various age. Investigations with a transfer of cells to irradiated and intact recipients were performed. We found a 23-fold diminution of the immune response in mice aged 26-28 months, when compared with that in mice aged 8 months. The first signs of a disturbed activity of the immune system were found in animals whose age did not exceed the half of their average lifespan. The injection of B-lymphocytes from young animals does not give rise to the immune response of old nonirradiated recipients. The injection of young b-lymphocytes to old irradiated recipients, being primed by antigen using Playfair's technique before irradiation, results in the highest response than it is in other combinations. The effect of disturbances in the interaction between  T- and B-cells on the mechanism of age-related changes of the immune response is discussed.", "contents": "Age-related changes of cells involved in immune responses. The interaction between T- and B-lymphocytes in the immune response to sheep reythrocytes was revealed by estimating the direct plaque-forming cells in mice CMA of various age. Investigations with a transfer of cells to irradiated and intact recipients were performed. We found a 23-fold diminution of the immune response in mice aged 26-28 months, when compared with that in mice aged 8 months. The first signs of a disturbed activity of the immune system were found in animals whose age did not exceed the half of their average lifespan. The injection of B-lymphocytes from young animals does not give rise to the immune response of old nonirradiated recipients. The injection of young b-lymphocytes to old irradiated recipients, being primed by antigen using Playfair's technique before irradiation, results in the highest response than it is in other combinations. The effect of disturbances in the interaction between  T- and B-cells on the mechanism of age-related changes of the immune response is discussed."}
{"id": "PMID:1091127", "title": "Effect of clofibrate on progression of coronary disease: a prospective angiographic study in man.", "content": "Lowering blood lipids has been invoked as a means of controlling future coronary events.  In this prospective study, the effect of a lipid-reducing agent clofibrate (2 gm. daily) on extent of coronary artery disease was investigated.  Forty patients, 32 having aortocoronary bypass, six having Vineberg operations, two having neither, were placed double-blind in placebo (24 patients) and clofibrate (16 patients) groups, and restudied by selective coronary angiography one year later.  An additional 24 patients dropped from the study due to adverse drug reactions in eight.  Each patient's right, left, anterior descending, and circumflex coronary arteries (with their branches) were separately rated according to degree of obstruction.  The clofibrate group showed a significantly greater fall in triglyceride than did the placebo group (minus 13.7 per cent versus plus 2.3 per cent; p equal to 0.45).  In the clofibrate group, 19 out of 64 coronary arteries (29.6 per cent) showed progressive coronary narrowing, not significantly different from the placebo group (24 out of 96 coronary arteries narrowed, 25 per cent; p equal to 0.26).  No significant differences between drug groups emerged when the data were corrected for degree of fall of blood lipids, initial lipoprotein type, or effect in bypassed versus nonbypassed vessels (p always greater than 0.2).  Regression of coronary artery disease was not seen.  We conclude that clofibrate did not significantly influence the rate of progression of coronary artery disease in a one-year period.", "contents": "Effect of clofibrate on progression of coronary disease: a prospective angiographic study in man. Lowering blood lipids has been invoked as a means of controlling future coronary events.  In this prospective study, the effect of a lipid-reducing agent clofibrate (2 gm. daily) on extent of coronary artery disease was investigated.  Forty patients, 32 having aortocoronary bypass, six having Vineberg operations, two having neither, were placed double-blind in placebo (24 patients) and clofibrate (16 patients) groups, and restudied by selective coronary angiography one year later.  An additional 24 patients dropped from the study due to adverse drug reactions in eight.  Each patient's right, left, anterior descending, and circumflex coronary arteries (with their branches) were separately rated according to degree of obstruction.  The clofibrate group showed a significantly greater fall in triglyceride than did the placebo group (minus 13.7 per cent versus plus 2.3 per cent; p equal to 0.45).  In the clofibrate group, 19 out of 64 coronary arteries (29.6 per cent) showed progressive coronary narrowing, not significantly different from the placebo group (24 out of 96 coronary arteries narrowed, 25 per cent; p equal to 0.26).  No significant differences between drug groups emerged when the data were corrected for degree of fall of blood lipids, initial lipoprotein type, or effect in bypassed versus nonbypassed vessels (p always greater than 0.2).  Regression of coronary artery disease was not seen.  We conclude that clofibrate did not significantly influence the rate of progression of coronary artery disease in a one-year period."}
{"id": "PMID:1091128", "title": "Ultrasound in the diagnosis of congenital heart disease.", "content": "In addition to recording the motion of the mitral, tricuspid, aortic, and pulmonic valves, echocardiography can identify right and left ventricular cavities and the interventricular septum.  Disorders such as atrial-septal defect, valvular and subvalvular aortic stenosis, pulmonic stenosis, Ebstein's anomaly of the tricuspid valve, and the hypoplastic left-heart syndrome can readily be evaluated by echocardiography.  In tetralogy of Fallot and truncus arteriosus, discontinuity between the anterior aortic wall and septum with overriding aorta has been demonstrated.  Doubleoutlet right ventricle is associated with posterior aortic wall and mitral valve discontinuity.  In disorders such as single ventricle, tricuspid atresia, and endocardial cushion defect with common A-V canal, echocardiographic demonstration of the absence of the interventricular septum has provided the clinician with valuable information.  Newer techniques such as compound-B ultrasonography, which produces a two-dimensional cross-sectional image of intracardiac structures, and multiscan echocardiography will enhance the use of conventional echocardiography by providing a more accurate anatomic display of cardiac chambers and outflow vessels.", "contents": "Ultrasound in the diagnosis of congenital heart disease. In addition to recording the motion of the mitral, tricuspid, aortic, and pulmonic valves, echocardiography can identify right and left ventricular cavities and the interventricular septum.  Disorders such as atrial-septal defect, valvular and subvalvular aortic stenosis, pulmonic stenosis, Ebstein's anomaly of the tricuspid valve, and the hypoplastic left-heart syndrome can readily be evaluated by echocardiography.  In tetralogy of Fallot and truncus arteriosus, discontinuity between the anterior aortic wall and septum with overriding aorta has been demonstrated.  Doubleoutlet right ventricle is associated with posterior aortic wall and mitral valve discontinuity.  In disorders such as single ventricle, tricuspid atresia, and endocardial cushion defect with common A-V canal, echocardiographic demonstration of the absence of the interventricular septum has provided the clinician with valuable information.  Newer techniques such as compound-B ultrasonography, which produces a two-dimensional cross-sectional image of intracardiac structures, and multiscan echocardiography will enhance the use of conventional echocardiography by providing a more accurate anatomic display of cardiac chambers and outflow vessels."}
{"id": "PMID:1091129", "title": "Electrophysiology and pharmacology of cardiac arrhythmias.  VI.  Cardiac effects of verapamil.", "content": "It is clear from clinical and experimental data that have been reported thus far that verapamil is highly effective in the therapy of cardiac arrhythmias, and that it acts by a different mechanism than most of the commonly used antiarrhythmic drugs.  The available clinical data indicate that on intravenous administration verapamil is as good as and perhaps superior to quinidine, procainamide and propranolol for the therapy of many atrial arrhythmias.  Unfortunately the extent to which it is useful as longterm prophylaxis has not yet been reported, nor has its toxicity during protracted oral administration.  The effects of verapamil on cardiac action potentials clearly indicate that it modifies the slow response to a much greater extent than the fast response.  Studies of cardiac tissues from diseased human atria have indicated that slow response action potentials occur frequently.  It is possible that such action potentials are responsible for the reentrant and automatic arrhythmias which occur in association with clinical cardiac disease.  Whether the efficacy of verapamil in the therapy of atrial arrhythmias is primarily due to abolishing slow response activity in diseased atrial tissues or to suppression of propagation through the atrioventricular node is uncertain.  However, it is likely that the therapeutic action of the drug may result from altered propagation of an arrhythmia through the atrioventricular junction as well as from the effects of the drug on diseased atrial and ventricular tissues.", "contents": "Electrophysiology and pharmacology of cardiac arrhythmias.  VI.  Cardiac effects of verapamil. It is clear from clinical and experimental data that have been reported thus far that verapamil is highly effective in the therapy of cardiac arrhythmias, and that it acts by a different mechanism than most of the commonly used antiarrhythmic drugs.  The available clinical data indicate that on intravenous administration verapamil is as good as and perhaps superior to quinidine, procainamide and propranolol for the therapy of many atrial arrhythmias.  Unfortunately the extent to which it is useful as longterm prophylaxis has not yet been reported, nor has its toxicity during protracted oral administration.  The effects of verapamil on cardiac action potentials clearly indicate that it modifies the slow response to a much greater extent than the fast response.  Studies of cardiac tissues from diseased human atria have indicated that slow response action potentials occur frequently.  It is possible that such action potentials are responsible for the reentrant and automatic arrhythmias which occur in association with clinical cardiac disease.  Whether the efficacy of verapamil in the therapy of atrial arrhythmias is primarily due to abolishing slow response activity in diseased atrial tissues or to suppression of propagation through the atrioventricular node is uncertain.  However, it is likely that the therapeutic action of the drug may result from altered propagation of an arrhythmia through the atrioventricular junction as well as from the effects of the drug on diseased atrial and ventricular tissues."}
{"id": "PMID:1091130", "title": "Effects of alpha-tocopherol acetate on the swimming endurance of trained swimmers.", "content": "Well-trained, competitive swimmers were divided into two groups. Group A was given 900 IU alpha-tocopherol acetate daily for 6 months while group B was given placebos. A swimming endurance test was given before the start of supplementation and after 1, 2, 5 and 6 months. No difference in swimmers' endurance was observed between the two groups during the 6-month period. There was also no difference in postexercise serum lactic acid levels. Younger, less well-trained, competitive swimmers were also divided into two groups. Group A received 900 IU alpha-tocopherol acetate daily while group B received placebos. Swimming times for these swimmers were erratic, reflecting a lack of training. alpha-Tocopherol did not appear to have any effect on their swimming endurance.", "contents": "Effects of alpha-tocopherol acetate on the swimming endurance of trained swimmers. Well-trained, competitive swimmers were divided into two groups. Group A was given 900 IU alpha-tocopherol acetate daily for 6 months while group B was given placebos. A swimming endurance test was given before the start of supplementation and after 1, 2, 5 and 6 months. No difference in swimmers' endurance was observed between the two groups during the 6-month period. There was also no difference in postexercise serum lactic acid levels. Younger, less well-trained, competitive swimmers were also divided into two groups. Group A received 900 IU alpha-tocopherol acetate daily while group B received placebos. Swimming times for these swimmers were erratic, reflecting a lack of training. alpha-Tocopherol did not appear to have any effect on their swimming endurance."}
{"id": "PMID:1091131", "title": "Physiology of the menstrual cycle.", "content": "Modern techniques of bioassay have permitted correlation of hormonal secretion with genital tissue changes during the normal menstrual cycle. During the follicular phase, estrogen secretion rises while other hormone levels are low. At ovulation luteinizing hormone and follicle-stimulating hormone surges are associated with falling estrogen levels. Secretions of progesterone and estrogen again are characteristic of the luteal phase ending with menstruation. Gonadotrophin-releasing hormones are detectable just before the luteinizing hormone and follicle-stimulating hormone surges. Basal body temperature rises with ovulation and is still the most reliable clinical indicator, although ferning and spinnbarkeit (when present) are also quite helpful. Vaginal smears are probably less useful except in the hands of experienced observers.", "contents": "Physiology of the menstrual cycle. Modern techniques of bioassay have permitted correlation of hormonal secretion with genital tissue changes during the normal menstrual cycle. During the follicular phase, estrogen secretion rises while other hormone levels are low. At ovulation luteinizing hormone and follicle-stimulating hormone surges are associated with falling estrogen levels. Secretions of progesterone and estrogen again are characteristic of the luteal phase ending with menstruation. Gonadotrophin-releasing hormones are detectable just before the luteinizing hormone and follicle-stimulating hormone surges. Basal body temperature rises with ovulation and is still the most reliable clinical indicator, although ferning and spinnbarkeit (when present) are also quite helpful. Vaginal smears are probably less useful except in the hands of experienced observers."}
{"id": "PMID:1091132", "title": "Oral contraceptive hormones, folate metabolism, and the cervical epithelium.", "content": "The currently available evidence concerning disorders of folate metabolism in women taking oral contraceptives has been reviewed. A disturbance in folate balance serious enough to cause symptoms (i.e., megaloblastic anemia) occurs very rarely. In some series, but not in others, serum and/or red cell folate concentrations have been reduced in oral contraceptive users. It is doubtful whether sex steroids affect polyglutamate folate absorption. About 20 percent of women taking contraceptive hormones manifest mild megaloblastic changes on Papanicolaou smears of the cervicovaginal epithelium which disappear after folic acid therapy. The current evidence, however, would not indicate that any significant benefit would ensue from routine folate supplementation in women on oral contraceptives.", "contents": "Oral contraceptive hormones, folate metabolism, and the cervical epithelium. The currently available evidence concerning disorders of folate metabolism in women taking oral contraceptives has been reviewed. A disturbance in folate balance serious enough to cause symptoms (i.e., megaloblastic anemia) occurs very rarely. In some series, but not in others, serum and/or red cell folate concentrations have been reduced in oral contraceptive users. It is doubtful whether sex steroids affect polyglutamate folate absorption. About 20 percent of women taking contraceptive hormones manifest mild megaloblastic changes on Papanicolaou smears of the cervicovaginal epithelium which disappear after folic acid therapy. The current evidence, however, would not indicate that any significant benefit would ensue from routine folate supplementation in women on oral contraceptives."}
{"id": "PMID:1091133", "title": "Adverse reactions, contraindications and complications of acupuncture and moxibustion.", "content": "This article discusses the various adverse reactions, contraindications and complications of employing acupuncture and moxibustion in therapy. A historical review of these phenomena is also presented, as well as a section on the side effects of auriculotherapy.", "contents": "Adverse reactions, contraindications and complications of acupuncture and moxibustion. This article discusses the various adverse reactions, contraindications and complications of employing acupuncture and moxibustion in therapy. A historical review of these phenomena is also presented, as well as a section on the side effects of auriculotherapy."}
{"id": "PMID:1091135", "title": "Diagnosis of human rabies by the cornea test.", "content": "The second death due to rabies encephalitis occurring among American servicemen stationed in the Republic of Vietnam is reported. The clinical diagnosis was confirmed by performing the direct fluorescent antibody test for rabies on smears of corneal epithelial cells obtained on the second day of hospitalization. The reliability of various laboratory procedures in confirming the diagnosis of human rabies early in the course of clinical illness is discussed. The cornea test is a useful and rapid method of diagnosing human rabies prior to the development of significant serum antibody titers.", "contents": "Diagnosis of human rabies by the cornea test. The second death due to rabies encephalitis occurring among American servicemen stationed in the Republic of Vietnam is reported. The clinical diagnosis was confirmed by performing the direct fluorescent antibody test for rabies on smears of corneal epithelial cells obtained on the second day of hospitalization. The reliability of various laboratory procedures in confirming the diagnosis of human rabies early in the course of clinical illness is discussed. The cornea test is a useful and rapid method of diagnosing human rabies prior to the development of significant serum antibody titers."}
{"id": "PMID:1091136", "title": "Detection of bacteriuria by dip-slide. Routine use in a large general hospital.", "content": "The use of a dip-slide (Uricult) procedure for the culture of voided urine specimens in a large general hospital was found to give equivalent results to those obtained by conventional culture technics. There was a significant decrease in the number of unsatisfactory speciments that needed to be repeated. The dip-slide procedure was readily accepted by physicians, nurses and laboratory personnel, and significant savings for the institution were realized.", "contents": "Detection of bacteriuria by dip-slide. Routine use in a large general hospital. The use of a dip-slide (Uricult) procedure for the culture of voided urine specimens in a large general hospital was found to give equivalent results to those obtained by conventional culture technics. There was a significant decrease in the number of unsatisfactory speciments that needed to be repeated. The dip-slide procedure was readily accepted by physicians, nurses and laboratory personnel, and significant savings for the institution were realized."}
{"id": "PMID:1091137", "title": "Computer data processing of medical diagnoses in pathology.", "content": "Modes of insertion of pathology diagnoses into a computer data storage and retrieval system are reviewed. The conversion of free-flowing diagnostic sentences into internal code is considered, and the advantages of coding are discussed from two aspects: (a) to minimize storage, and (b) to help alleviate difficulties in retrieval of synonymous terminology. Methods of manually pre-coding diagnoses into Systemized Nomenclature of Pathology (SNOP) code are discussed. Data encoding produces a fixed format record which provides significant economy in data handling. The potential use of a real-time visual display unit in data gathering and automatic coding is presented.", "contents": "Computer data processing of medical diagnoses in pathology. Modes of insertion of pathology diagnoses into a computer data storage and retrieval system are reviewed. The conversion of free-flowing diagnostic sentences into internal code is considered, and the advantages of coding are discussed from two aspects: (a) to minimize storage, and (b) to help alleviate difficulties in retrieval of synonymous terminology. Methods of manually pre-coding diagnoses into Systemized Nomenclature of Pathology (SNOP) code are discussed. Data encoding produces a fixed format record which provides significant economy in data handling. The potential use of a real-time visual display unit in data gathering and automatic coding is presented."}
{"id": "PMID:1091138", "title": "Dietary supplement and nutrition in children with cystic fibrosis.", "content": "Assessment of nutritional status of patients with cystic fibrosis of the pancreas (CFP) showed that poor growth was associated with low concentrations of albumin, urea nitrogen, and cholesterol in serum and with elevated white blood cell (WBC) counts. Patients with CFP maintained weight approximately 1 standard deviation below the mean until 8 years, after which there was a progressive decline in growth rate compared to normal. A complete dietary supplement consisting of a beef serum hydrolysate, a glucose polymer, and medium-chain triglycerides was given to 15 patients for a year. Patients who received the diet showed significant gains in weight, significant increase in clinical score, significant increase in serum albumin level, and a significant drop in the WBC count compared to control patients who did not receive the supplement.", "contents": "Dietary supplement and nutrition in children with cystic fibrosis. Assessment of nutritional status of patients with cystic fibrosis of the pancreas (CFP) showed that poor growth was associated with low concentrations of albumin, urea nitrogen, and cholesterol in serum and with elevated white blood cell (WBC) counts. Patients with CFP maintained weight approximately 1 standard deviation below the mean until 8 years, after which there was a progressive decline in growth rate compared to normal. A complete dietary supplement consisting of a beef serum hydrolysate, a glucose polymer, and medium-chain triglycerides was given to 15 patients for a year. Patients who received the diet showed significant gains in weight, significant increase in clinical score, significant increase in serum albumin level, and a significant drop in the WBC count compared to control patients who did not receive the supplement."}
{"id": "PMID:1091139", "title": "Computer-assisted pediatric diagnosis.", "content": "A system for computer aid to diagnosis was tested on 90 children hospitalized with systemic illness and discharged with a final diagnosis. A medical secretary and a pediatrician were equally successful (78% vs 77%) in having the system list the main final diagnosis when each independently supplied a list of details extracted only from the pediatric intern's admission notes. Even when the final diagnosis was not considered by the pediatric house staff and attending physicians caring for the patient until after the day of admission, the system was accurate in listing the diagnosis (or in raising the correct line of diagnostic inquiry) in at least 85% of the patients.", "contents": "Computer-assisted pediatric diagnosis. A system for computer aid to diagnosis was tested on 90 children hospitalized with systemic illness and discharged with a final diagnosis. A medical secretary and a pediatrician were equally successful (78% vs 77%) in having the system list the main final diagnosis when each independently supplied a list of details extracted only from the pediatric intern's admission notes. Even when the final diagnosis was not considered by the pediatric house staff and attending physicians caring for the patient until after the day of admission, the system was accurate in listing the diagnosis (or in raising the correct line of diagnostic inquiry) in at least 85% of the patients."}
{"id": "PMID:1091140", "title": "Impact of a system of computer-assisted diagnosis. Initial evaluation of the hospitalized patient.", "content": "Computer assisted diagnosis was evaluated for its ability to hasten the time to initial diagnosis and reduce laboratory studies. The time to diagnosis averaged 2.8 days for a control group and 1.9 days for the group in which the computer printout was made available. In a small group in whom the diagnosis was not established until after 48 hours, there was a further trend to reduction in number of days to diagnosis and irrelevant laboratory studies. The ability of the computer to include the final diagnosis in the initial list of differential diagnoses remained unchanged for patients whose conditions were diagnosed before or after 48 hours, while the physicians' ability decreased. In a university hospital setting, computer-assisted diagnosis may be most beneficial for patients whose conditions have not been diagnosed within 48 hours of admission.", "contents": "Impact of a system of computer-assisted diagnosis. Initial evaluation of the hospitalized patient. Computer assisted diagnosis was evaluated for its ability to hasten the time to initial diagnosis and reduce laboratory studies. The time to diagnosis averaged 2.8 days for a control group and 1.9 days for the group in which the computer printout was made available. In a small group in whom the diagnosis was not established until after 48 hours, there was a further trend to reduction in number of days to diagnosis and irrelevant laboratory studies. The ability of the computer to include the final diagnosis in the initial list of differential diagnoses remained unchanged for patients whose conditions were diagnosed before or after 48 hours, while the physicians' ability decreased. In a university hospital setting, computer-assisted diagnosis may be most beneficial for patients whose conditions have not been diagnosed within 48 hours of admission."}
{"id": "PMID:1091141", "title": "Shunt fluid aspiration: an adjunct in the diagnosis of cerebrospinal fluid shunt infection.", "content": "Bacterial isolates were obtained from 42 of 59 cultures of shunt fluid aspirated from patients with infected cerebrospinal fluid shunts. The etiologic agent was recovered from 24 to 25 aspirates from patients who were not receiving antibiotics and from 18 of 34 aspirates from patients who were receiving antibiotics. Aspiration, culture, and Gram stain of shunt fluid should be considered in patients suspected of having a shunt infection.", "contents": "Shunt fluid aspiration: an adjunct in the diagnosis of cerebrospinal fluid shunt infection. Bacterial isolates were obtained from 42 of 59 cultures of shunt fluid aspirated from patients with infected cerebrospinal fluid shunts. The etiologic agent was recovered from 24 to 25 aspirates from patients who were not receiving antibiotics and from 18 of 34 aspirates from patients who were receiving antibiotics. Aspiration, culture, and Gram stain of shunt fluid should be considered in patients suspected of having a shunt infection."}
{"id": "PMID:1091142", "title": "Thoracic arteriovenous fistula: venous connection to right iliac vein.", "content": "A 5-year-old boy had clinical, cardiac catheterization, and operative findings of a thoracic arteriovenous fistula arising from the right subclavian artery. The venous connection was to the paravertebral plexus with eventual drainage into the inferior vena cava through the right common iliac vein. To our knowledge, this type of fistula has not been reported previously and is believed to represent embryological defects of the first or second posterior intercostal arteries as well as maldevelopment of the venous connections of azygos vein to the superior vena cava, which resulted in the observed caudal venous pathways becoming operative.", "contents": "Thoracic arteriovenous fistula: venous connection to right iliac vein. A 5-year-old boy had clinical, cardiac catheterization, and operative findings of a thoracic arteriovenous fistula arising from the right subclavian artery. The venous connection was to the paravertebral plexus with eventual drainage into the inferior vena cava through the right common iliac vein. To our knowledge, this type of fistula has not been reported previously and is believed to represent embryological defects of the first or second posterior intercostal arteries as well as maldevelopment of the venous connections of azygos vein to the superior vena cava, which resulted in the observed caudal venous pathways becoming operative."}
{"id": "PMID:1091143", "title": "The use of antibiotics in biliary tract disease.", "content": "Antibotics are often used in the treatment of patients with biliary tract disease. Certain drugs are relatively contraindicated if there is preexisting liver dysfunction. Not all antibiotis achieve a high level in bile, and  knowledge of their individual excretion rate is important for intelligent use.", "contents": "The use of antibiotics in biliary tract disease. Antibotics are often used in the treatment of patients with biliary tract disease. Certain drugs are relatively contraindicated if there is preexisting liver dysfunction. Not all antibiotis achieve a high level in bile, and  knowledge of their individual excretion rate is important for intelligent use."}
{"id": "PMID:1091144", "title": "The use of metoclopramide in acute pyloric obstruction.  A clinical trial.", "content": "Twelve patients with complete, or nearly complete, pyloric obstruction due to duodenal ulcer, were treated conservatively with metoclopramide.  The purpose of this therapeutic trial was to avoid emergency operations in these patients and to enable a better radiological evaluation and clinical preparation before surgery.  The treatment, in addition to the usual gastric decompression and fluid and electrolyte replacement therapy, was effective in 10 patients (83.4 percent).  In eight of these patients the results of the trial was regarded as \"very good\".  Six of them were operated upon electively later and two were not operated upon at all.  In two patients the results were regarded as \"good\".  These patients underwent early elective surgery.  In the remaining two patients (16.6 percent) the trial failed and semiurgent operation could not be avoided.  As no side-effects are evident following the administration of metoclopramide and no harm is done by delaying surgery for three days, it is suggested that this therapeutic trial should be undertaken in every case of pyloric stenosis or obstruction.  This may markedly reduce the need for emergency operation and prevent many postoperative complications which not uncommonly follow emergency surgery for pyloric stenosis.", "contents": "The use of metoclopramide in acute pyloric obstruction.  A clinical trial. Twelve patients with complete, or nearly complete, pyloric obstruction due to duodenal ulcer, were treated conservatively with metoclopramide.  The purpose of this therapeutic trial was to avoid emergency operations in these patients and to enable a better radiological evaluation and clinical preparation before surgery.  The treatment, in addition to the usual gastric decompression and fluid and electrolyte replacement therapy, was effective in 10 patients (83.4 percent).  In eight of these patients the results of the trial was regarded as \"very good\".  Six of them were operated upon electively later and two were not operated upon at all.  In two patients the results were regarded as \"good\".  These patients underwent early elective surgery.  In the remaining two patients (16.6 percent) the trial failed and semiurgent operation could not be avoided.  As no side-effects are evident following the administration of metoclopramide and no harm is done by delaying surgery for three days, it is suggested that this therapeutic trial should be undertaken in every case of pyloric stenosis or obstruction.  This may markedly reduce the need for emergency operation and prevent many postoperative complications which not uncommonly follow emergency surgery for pyloric stenosis."}
{"id": "PMID:1091146", "title": "Female excess in anencephaly and spina bifida: possible gestational influences.", "content": "Data are presented on th sex-specific frequency and the sex ratios of anencephaly and spina bifida from 1945 through 1971. Dramatic changes in the overall rate of these malformations occurred during that period. The purpose of this study was to evaluate the concept of sex-specific thresholds for an unidentified environmental teratogen, a concept which had recently been proposed. The observed epidemiologic relationship between the frequencies and the sex ratios of these malformations is too complex to reconcile with the action of a single teratogen. On hypothetical grounds, a deficiency of human chorionic gonadotrophin (HCG) could explain the sex ratio pattern. These malformations might be caused by yet unidentified factors which produce a deficiency in HCG function. These factors could be placental teratogens, or they could be some other type of environmental agent which is capable of producing a long-term effect on maternal endocrine function.", "contents": "Female excess in anencephaly and spina bifida: possible gestational influences. Data are presented on th sex-specific frequency and the sex ratios of anencephaly and spina bifida from 1945 through 1971. Dramatic changes in the overall rate of these malformations occurred during that period. The purpose of this study was to evaluate the concept of sex-specific thresholds for an unidentified environmental teratogen, a concept which had recently been proposed. The observed epidemiologic relationship between the frequencies and the sex ratios of these malformations is too complex to reconcile with the action of a single teratogen. On hypothetical grounds, a deficiency of human chorionic gonadotrophin (HCG) could explain the sex ratio pattern. These malformations might be caused by yet unidentified factors which produce a deficiency in HCG function. These factors could be placental teratogens, or they could be some other type of environmental agent which is capable of producing a long-term effect on maternal endocrine function."}
{"id": "PMID:1091148", "title": "The effectiveness of human placental lactogen measurements as an adjunct in decreasing perinatal deaths. Results of a retrospective and a randomized controlled prospective study.", "content": "Serum human placental lactogen (HPL) levels were measured in high-risk pregnancies. A retrospective analysis of 101 gestations with a perinatal loss showed that the 71 stillbirths had abnormally low fetal-danger zone (F-D zone) HPL levels (50.7%) before the fetus died (normal smaller than 1%). With severe hypertension, 85.7% of the cases had F-D zone HPL values prior to fetal death. Abnormal low F-D zone HPL values were found in 20% of the 30 neonatal deaths. A 3 year randomized controlled treatment series was done with 2,7333 women (1,362 treatment and 1,371 control) who had serial serum HPL measurements performed at each prenatal visit. The frequency of F-D zone HPL values was similar (8.6% treatment and 8.2% control). The results showed that although the neonatal mortality rates were similar, the HPL treatment group had a significantly lower fetal death rate (2.6 vs. 14.2%) and thus perinatal mortality rate (3.4 vs. 15.0%). This study supports the usefulness of routine prenatal HPL measurements in the management of high-risk pregnancies.", "contents": "The effectiveness of human placental lactogen measurements as an adjunct in decreasing perinatal deaths. Results of a retrospective and a randomized controlled prospective study. Serum human placental lactogen (HPL) levels were measured in high-risk pregnancies. A retrospective analysis of 101 gestations with a perinatal loss showed that the 71 stillbirths had abnormally low fetal-danger zone (F-D zone) HPL levels (50.7%) before the fetus died (normal smaller than 1%). With severe hypertension, 85.7% of the cases had F-D zone HPL values prior to fetal death. Abnormal low F-D zone HPL values were found in 20% of the 30 neonatal deaths. A 3 year randomized controlled treatment series was done with 2,7333 women (1,362 treatment and 1,371 control) who had serial serum HPL measurements performed at each prenatal visit. The frequency of F-D zone HPL values was similar (8.6% treatment and 8.2% control). The results showed that although the neonatal mortality rates were similar, the HPL treatment group had a significantly lower fetal death rate (2.6 vs. 14.2%) and thus perinatal mortality rate (3.4 vs. 15.0%). This study supports the usefulness of routine prenatal HPL measurements in the management of high-risk pregnancies."}
{"id": "PMID:1091149", "title": "Guidelines for antibiotic prophylaxis in gynecology.", "content": "Using a random table of numbers, 100 premenopausal women undergoing vaginal hysterectomy were assigned to a short-term or long-term antibiotic prophylaxis regimen. The short-term regimen patients received a total of 3 Gm. of cephaloridine in divided doses on the day of operation, while the long-term regimen patients also received oral cephalexin postoperatively. The preoperative profiles of the two study groups were similar, and the postoperative results were not significantly different. A quantitative evaluation of aerobic and anaerobic bacteria recovered from the surface of the vagina was done, and cephaloridine content of vaginal washings, serum, and vaginal tissue was determined. This information was utilized to suggest guidelines for antibiotic prophylaxis in gynecology.", "contents": "Guidelines for antibiotic prophylaxis in gynecology. Using a random table of numbers, 100 premenopausal women undergoing vaginal hysterectomy were assigned to a short-term or long-term antibiotic prophylaxis regimen. The short-term regimen patients received a total of 3 Gm. of cephaloridine in divided doses on the day of operation, while the long-term regimen patients also received oral cephalexin postoperatively. The preoperative profiles of the two study groups were similar, and the postoperative results were not significantly different. A quantitative evaluation of aerobic and anaerobic bacteria recovered from the surface of the vagina was done, and cephaloridine content of vaginal washings, serum, and vaginal tissue was determined. This information was utilized to suggest guidelines for antibiotic prophylaxis in gynecology."}
{"id": "PMID:1091150", "title": "Cooperative clinical trials. The Second Ticho Memorial Lecture.", "content": "As exemplified by the collaborative Diabetic Retinopathy Study, all the appropriate techniques involved in a clinical trial-random assignment of treatment, appropriate controls, and avoidance of investigator bias-should be incorporated during the earliest testing of a new therapy for a chronic disease, for the sake of the patient and for the progress of medical science. This approach to clinical trials in the testing of new ocular therapies can provide the most scientifically sound and rational therapy to the patient.", "contents": "Cooperative clinical trials. The Second Ticho Memorial Lecture. As exemplified by the collaborative Diabetic Retinopathy Study, all the appropriate techniques involved in a clinical trial-random assignment of treatment, appropriate controls, and avoidance of investigator bias-should be incorporated during the earliest testing of a new therapy for a chronic disease, for the sake of the patient and for the progress of medical science. This approach to clinical trials in the testing of new ocular therapies can provide the most scientifically sound and rational therapy to the patient."}
{"id": "PMID:1091151", "title": "Intravenous adenine arabinoside against herpes simplex keratouveitis in humans.", "content": "Adenine arabinoside possessed all of the desirable characteristics for an antiviral agent: (1) not incorporated into host DNA; (2) broad spectrum activity against the DNA family of viruses; (3) lack of resistance in vitro; (4) rapid excretion; (5) did not cause immunosuppression; (6) low toxicity in significantly effective doses; and (m7) relatively inexpensive. Adenine arabinoside employed in 20 mg/kg1day intravenous infusions for seven days in a controlled series of patients was clearly effective in treating herpetic keratouveitis with only minimal adverse reactions. For the first time there may be an effective specific treatment for herpetic stromal disease and iritis. Further controlled studies comparing the effectiveness of adenine arabinoside with and without concommitant topical corticosteroid therapy are necessary.", "contents": "Intravenous adenine arabinoside against herpes simplex keratouveitis in humans. Adenine arabinoside possessed all of the desirable characteristics for an antiviral agent: (1) not incorporated into host DNA; (2) broad spectrum activity against the DNA family of viruses; (3) lack of resistance in vitro; (4) rapid excretion; (5) did not cause immunosuppression; (6) low toxicity in significantly effective doses; and (m7) relatively inexpensive. Adenine arabinoside employed in 20 mg/kg1day intravenous infusions for seven days in a controlled series of patients was clearly effective in treating herpetic keratouveitis with only minimal adverse reactions. For the first time there may be an effective specific treatment for herpetic stromal disease and iritis. Further controlled studies comparing the effectiveness of adenine arabinoside with and without concommitant topical corticosteroid therapy are necessary."}
{"id": "PMID:1091152", "title": "Metabolic bone disease in chronic renal failure. II. Renal transplant patients.", "content": "Trabecular vertebral bone of renal transplant patients was quantitatively compared with bone from normal individuals and dialyzed and nondialyzed patienets with chronic renal failure reported in detail in an earlier study. Long- and short-term transplant patients have increased bone resorption and mineralization defects similar to renal osteodystrophy in dialyzed and nondialyzed patients. However, in transplant patients the magnitude of resorption is greater, and bone volume tends to decrease rather than increase. Resorptive activity in transplant patients is maximal during the first year after transplantation. Bone volume decreases continuously for at least 96 months after transplantation. Only decreased bone volume correlated with success or failure of the renal transplant. Morphologic findings in this study correlate with other clinical and morphologic data to suggest that reduction in bone volume in transplant patients results from a combination of persistent hyperparathyroidism and suppression of bone formation by steroid therapy.", "contents": "Metabolic bone disease in chronic renal failure. II. Renal transplant patients. Trabecular vertebral bone of renal transplant patients was quantitatively compared with bone from normal individuals and dialyzed and nondialyzed patienets with chronic renal failure reported in detail in an earlier study. Long- and short-term transplant patients have increased bone resorption and mineralization defects similar to renal osteodystrophy in dialyzed and nondialyzed patients. However, in transplant patients the magnitude of resorption is greater, and bone volume tends to decrease rather than increase. Resorptive activity in transplant patients is maximal during the first year after transplantation. Bone volume decreases continuously for at least 96 months after transplantation. Only decreased bone volume correlated with success or failure of the renal transplant. Morphologic findings in this study correlate with other clinical and morphologic data to suggest that reduction in bone volume in transplant patients results from a combination of persistent hyperparathyroidism and suppression of bone formation by steroid therapy."}
{"id": "PMID:1091153", "title": "Effects of intravascular clotting on the activation of the complement system: The role of the platelet.", "content": "Total hemolytic complement activity and the third component of complement were found to be significantly depressed in vivo in rabbits following the induction of disseminated intravascular coagulation by both thrombin and thromboplastin. Production of severe thrombocytopenia by the administration of platelet antiserum prior to the infusion of thrombin or thromboplastin partially prevented complement activation. The data show that, when clotting is triggered, complement activation takes place and that platelets are required to some extent for this reaction.", "contents": "Effects of intravascular clotting on the activation of the complement system: The role of the platelet. Total hemolytic complement activity and the third component of complement were found to be significantly depressed in vivo in rabbits following the induction of disseminated intravascular coagulation by both thrombin and thromboplastin. Production of severe thrombocytopenia by the administration of platelet antiserum prior to the infusion of thrombin or thromboplastin partially prevented complement activation. The data show that, when clotting is triggered, complement activation takes place and that platelets are required to some extent for this reaction."}
{"id": "PMID:1091158", "title": "Pancreatic insulin release and peripheral tissue resistance in Zucker obese rats fed high- and low-carbohydrate diets.", "content": "An effort was made to determine to what degree the hyperinsulinemia found in the genetically obese Zucker rat is the result of the carbohydrate content of the diet. When Zucker obese rats are fed precisely the same amount of carbohydrate as lean controls and allowed to become obese by drinking vegetable oil, their pancreatic islets still release 59% more insulin than do those from lean controls. When their diet contains even more carbohydrate, fed from weaning, and they become equivalently obese, their islet insulin release is increased by an additional 46%. An obese Zucker rat fed a high-carbohydrate diet possesses muscle sensitivity to insulin and enlarged adipocytes undergoing active lipogenesis. A rat becoming equivalently obese on a high-fat diet has an absence of insulin sensitivity in muscle and diminished lipogenesis in  adipocytes. Clearly, the composition of the diet plays an important role in the metabolic consequences of obesity, but neither  diet nor changes in peripheral glucose metabolism can completely explain the hyperinsulinemia.", "contents": "Pancreatic insulin release and peripheral tissue resistance in Zucker obese rats fed high- and low-carbohydrate diets. An effort was made to determine to what degree the hyperinsulinemia found in the genetically obese Zucker rat is the result of the carbohydrate content of the diet. When Zucker obese rats are fed precisely the same amount of carbohydrate as lean controls and allowed to become obese by drinking vegetable oil, their pancreatic islets still release 59% more insulin than do those from lean controls. When their diet contains even more carbohydrate, fed from weaning, and they become equivalently obese, their islet insulin release is increased by an additional 46%. An obese Zucker rat fed a high-carbohydrate diet possesses muscle sensitivity to insulin and enlarged adipocytes undergoing active lipogenesis. A rat becoming equivalently obese on a high-fat diet has an absence of insulin sensitivity in muscle and diminished lipogenesis in  adipocytes. Clearly, the composition of the diet plays an important role in the metabolic consequences of obesity, but neither  diet nor changes in peripheral glucose metabolism can completely explain the hyperinsulinemia."}
{"id": "PMID:1091159", "title": "Psychotherapy: historical roots, universal elements, and cultural variations.", "content": "The authors review various forms of traditional psychotherapeutic interventions to trace the historical development of psychotherapy, which demonstrates a shift in focus from the supernatural world, to the natural world, to the physical person, then to the psychological person. The basic processes of identifying problems, providing explanations, and prescribing for change are observed among various kinds of treatment systems. Universal elements fundamental to successful treatment can be identified in each of these processes, and each is strongly modified by cultural factors. The authors conclude that the special cultural dimension of psychotherapy consists of defining cultural norms, reinforcing culturally sanctioned coping mechanisms, and providing \"time out\" from usual cultural expectations.", "contents": "Psychotherapy: historical roots, universal elements, and cultural variations. The authors review various forms of traditional psychotherapeutic interventions to trace the historical development of psychotherapy, which demonstrates a shift in focus from the supernatural world, to the natural world, to the physical person, then to the psychological person. The basic processes of identifying problems, providing explanations, and prescribing for change are observed among various kinds of treatment systems. Universal elements fundamental to successful treatment can be identified in each of these processes, and each is strongly modified by cultural factors. The authors conclude that the special cultural dimension of psychotherapy consists of defining cultural norms, reinforcing culturally sanctioned coping mechanisms, and providing \"time out\" from usual cultural expectations."}
{"id": "PMID:1091160", "title": "Current and emerging models of residential psychiatric treatment, with special reference to the California situation.", "content": "The author reviews the steps that have led to the progressive deemphasis of psychiatric hospitalization in California and surveys the empirical basis for rejecting the usefulness of hospitalization. He discusses the four alternative residential models that have emerged in California as substitutes for state mental hospitals--general hospital psychiatric units, board and care homes, private psychiatric facilities available through vendor contracts, and convalescent hospitals. The author also touches briefly upon some important problems, including the possible fate of public general hospital psychiatric units, community mental health services, and the commitment law in California.", "contents": "Current and emerging models of residential psychiatric treatment, with special reference to the California situation. The author reviews the steps that have led to the progressive deemphasis of psychiatric hospitalization in California and surveys the empirical basis for rejecting the usefulness of hospitalization. He discusses the four alternative residential models that have emerged in California as substitutes for state mental hospitals--general hospital psychiatric units, board and care homes, private psychiatric facilities available through vendor contracts, and convalescent hospitals. The author also touches briefly upon some important problems, including the possible fate of public general hospital psychiatric units, community mental health services, and the commitment law in California."}
{"id": "PMID:1091161", "title": "Depression and anxiety in heroin addicts: a placebo-controlled study of doxepin in combination with methadone.", "content": "Narcotic addicts may manifest symptoms of depression that aggravate their addiction. In this double-blind study of 35 mildly depressed patients in a methadone maintenance program, subjects who received doxepin improved significantly more than control subjects. Even in this short-term study, the reduction in depression was associated with a trend toward better performance in other areas of rehabilitation.", "contents": "Depression and anxiety in heroin addicts: a placebo-controlled study of doxepin in combination with methadone. Narcotic addicts may manifest symptoms of depression that aggravate their addiction. In this double-blind study of 35 mildly depressed patients in a methadone maintenance program, subjects who received doxepin improved significantly more than control subjects. Even in this short-term study, the reduction in depression was associated with a trend toward better performance in other areas of rehabilitation."}
{"id": "PMID:1091165", "title": "The prevalence and relationships of malaria, anemia, and malnutrition in a coastal area of El Salvador.", "content": "To study the relationships between malaria, anemia and malnutrition, 853 school-age children from a high malaria incidence area and an adjacent low incidence area were surveyed in September 1972. For the high incidence area the malaria slide positivity rate was 3.5%, spleen rate 7.6% and malaria (indirect fluorescent antibody) serology positivity 24.7%. Contrasted to this, no positive slides, only 3 palpable spleens and a 3.4% serology positivity rate were found for the low incidence area. Twenty-three percent of those studied were anemic, but the prevalence of anemia was the same in both the high and low incidence areas. However, a selected group of children with known history of recent or actual malaria was found to be more likely to have deficient hematocrit values than were children without such history. Hypochromia and microcytosis were prominent morphologic findings in children with anemia, suggesting a diagnosis of iron deficiency. Weights and heights for age were considerably below those of a U.S. reference population but similar to nationwide Salvadoran figures. In both the high and low incidence groups, 62% had arm circumference values below 90% of standard. The distribution of weight-to-height ratios was also similar for both groups. No difference in nutritional status between the two groups could be found.", "contents": "The prevalence and relationships of malaria, anemia, and malnutrition in a coastal area of El Salvador. To study the relationships between malaria, anemia and malnutrition, 853 school-age children from a high malaria incidence area and an adjacent low incidence area were surveyed in September 1972. For the high incidence area the malaria slide positivity rate was 3.5%, spleen rate 7.6% and malaria (indirect fluorescent antibody) serology positivity 24.7%. Contrasted to this, no positive slides, only 3 palpable spleens and a 3.4% serology positivity rate were found for the low incidence area. Twenty-three percent of those studied were anemic, but the prevalence of anemia was the same in both the high and low incidence areas. However, a selected group of children with known history of recent or actual malaria was found to be more likely to have deficient hematocrit values than were children without such history. Hypochromia and microcytosis were prominent morphologic findings in children with anemia, suggesting a diagnosis of iron deficiency. Weights and heights for age were considerably below those of a U.S. reference population but similar to nationwide Salvadoran figures. In both the high and low incidence groups, 62% had arm circumference values below 90% of standard. The distribution of weight-to-height ratios was also similar for both groups. No difference in nutritional status between the two groups could be found."}
{"id": "PMID:1091166", "title": "Rodent systems (Plasmodium berghei-Anopheles Stephensi) for screening compounds for potential causal prophylaxis.", "content": "An in vivo screening system is described in which drugs administered to rats or mice and challenged with sporozoites are evaluated for their antimalarial properties (causal prophylaxis, suppression, therapy) by the presence or absence of exoerythrocytic forms and parasitemia. The system is composed of A/J mice, Sprague-Dawley rats, Plasmodium berghei, and Anopheles stephensi. Good correlation has been found between test results and practical application.", "contents": "Rodent systems (Plasmodium berghei-Anopheles Stephensi) for screening compounds for potential causal prophylaxis. An in vivo screening system is described in which drugs administered to rats or mice and challenged with sporozoites are evaluated for their antimalarial properties (causal prophylaxis, suppression, therapy) by the presence or absence of exoerythrocytic forms and parasitemia. The system is composed of A/J mice, Sprague-Dawley rats, Plasmodium berghei, and Anopheles stephensi. Good correlation has been found between test results and practical application."}
{"id": "PMID:1091167", "title": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. I. Study design and perspective.", "content": "A large-scale prospective study was designed to test the effects of aerial ultralow volume (ULV) application of malathion on epidemic Plasmodium falciparum malaria. The study was conducted during 1972 to 1973, in the Miragoane Valley of Haiti, an area having annual anticipated outbreaks of malaria, which allowed prospective assessment. Spraying of malathion at a dosage of 4.5 fluid ounces per acre reduced populations of adult Anopheles albimanus to less than 1% of prespray levels and interrupted epidemic transmission of P. falciparum malaria. No change was measured in susceptibility of the vector mosquito to malathion after six applications of spray during a period of 50 days. Ecologic study revealed no significant impact on nontarget vertebrates. Factors that contributed to the success of this method in Haiti were: 1) a susceptible population of mosquitoes; 2) suitable topography and climate conditions for spraying; and 3) treatment of an area sufficiently large to minimize the influence of immigration of mosquitoes from unsprayed areas.", "contents": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. I. Study design and perspective. A large-scale prospective study was designed to test the effects of aerial ultralow volume (ULV) application of malathion on epidemic Plasmodium falciparum malaria. The study was conducted during 1972 to 1973, in the Miragoane Valley of Haiti, an area having annual anticipated outbreaks of malaria, which allowed prospective assessment. Spraying of malathion at a dosage of 4.5 fluid ounces per acre reduced populations of adult Anopheles albimanus to less than 1% of prespray levels and interrupted epidemic transmission of P. falciparum malaria. No change was measured in susceptibility of the vector mosquito to malathion after six applications of spray during a period of 50 days. Ecologic study revealed no significant impact on nontarget vertebrates. Factors that contributed to the success of this method in Haiti were: 1) a susceptible population of mosquitoes; 2) suitable topography and climate conditions for spraying; and 3) treatment of an area sufficiently large to minimize the influence of immigration of mosquitoes from unsprayed areas."}
{"id": "PMID:1091168", "title": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. II. Entomologic and operational aspects.", "content": "In a large-scale study in the Miragoane Valley of Haiti, designed to test the effects of aerial ultralow volume (ULV) malathion on epidemic Plasmodium falciparum malaria, spray operations resulted in an immediate and sharp decline in numbers of the vector, Anopheles albimanus. The adult population of this mosquito remained at less than 1% of previous levels until several weeks after a 50-day spray period (27 October-16 December 1972) during which six cycles were completed. The study area offered ideal conditions of wind, temperature, humidity, and mountain barriers. Mosquitoes in the area were highly susceptible to malathion. Results indicated that aerial ULV treatment with malathion can reduce A. albimanus populations rapidly and effectively when applications are made over an area as large as 20,000 acres. Preliminary results showed that effective control was not achieved in areas one-quarter that size; these areas were not sufficiently large, and infiltration of mosquitoes from adjacent untreated areas was possible.", "contents": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. II. Entomologic and operational aspects. In a large-scale study in the Miragoane Valley of Haiti, designed to test the effects of aerial ultralow volume (ULV) malathion on epidemic Plasmodium falciparum malaria, spray operations resulted in an immediate and sharp decline in numbers of the vector, Anopheles albimanus. The adult population of this mosquito remained at less than 1% of previous levels until several weeks after a 50-day spray period (27 October-16 December 1972) during which six cycles were completed. The study area offered ideal conditions of wind, temperature, humidity, and mountain barriers. Mosquitoes in the area were highly susceptible to malathion. Results indicated that aerial ULV treatment with malathion can reduce A. albimanus populations rapidly and effectively when applications are made over an area as large as 20,000 acres. Preliminary results showed that effective control was not achieved in areas one-quarter that size; these areas were not sufficiently large, and infiltration of mosquitoes from adjacent untreated areas was possible."}
{"id": "PMID:1091169", "title": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. III. Ecologic aspects.", "content": "The effects of aerial ultralow volume (ULV) malathion on selected species of nontarget animals in Haiti are reported. Mortality of certain groups of insects such as bees, flies, beetles, and butterflies was observed immediately following spray application. Minor fish mortality occurred only in shallow water exposed to direct spray. The brain acetylcholine esterase levels of living fish, tree lizards, birds and bats collected from treated areas were not significantly reduced. No ill or dead animals, besides the few fish, were seen even when maximum exposure occurred. Only minor changes in the feeding behavior of some insectivorous birds were observed. The relative bird density decreased substantially for only one species during the study, and other factors besides treatment were considered to be the reasons for the decline. Aerial applications of ULV malathion at dosages sufficient to dramatically reduce anopheline populations did not significantly affect nontarget vertebrates in this tropical environment.", "contents": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. III. Ecologic aspects. The effects of aerial ultralow volume (ULV) malathion on selected species of nontarget animals in Haiti are reported. Mortality of certain groups of insects such as bees, flies, beetles, and butterflies was observed immediately following spray application. Minor fish mortality occurred only in shallow water exposed to direct spray. The brain acetylcholine esterase levels of living fish, tree lizards, birds and bats collected from treated areas were not significantly reduced. No ill or dead animals, besides the few fish, were seen even when maximum exposure occurred. Only minor changes in the feeding behavior of some insectivorous birds were observed. The relative bird density decreased substantially for only one species during the study, and other factors besides treatment were considered to be the reasons for the decline. Aerial applications of ULV malathion at dosages sufficient to dramatically reduce anopheline populations did not significantly affect nontarget vertebrates in this tropical environment."}
{"id": "PMID:1091170", "title": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. IV. Epidemiologic aspects.", "content": "In the Miragoane Valley of Haiti a consistent pattern in the incidence of Plasmodium falciparum malaria over a 10-year period made it possible to predict an annual outbreak and perform a prospective study to test the effects of aerial ultralow volume (ULV) malathion on epidemic levels of this disease. At the end of October 1972, after epidemic levels (100 cases/month/10,000 population) had been reached, spray operations were begun. The first spray cycle produced a sharp and immediate drop in populations of the vector Anopheles albimanus, followed 4 weeks later by a decrease in the incidence of malaria throughout the valley. Although the incidence of malaria was similar in sprayed and unsprayed areas prior to the effect of ULV malathion (176.1 and 198.7 cases/month/10,000 population, respectively), it was significantly different during the subsequent 3 months (16.8 cases/month/10,000 population in sprayed areas and 65.4 in unsprayed; p less than 0.001). Travel histories indicated that only 4% of all cases had spent a night away from home during the 4 weeks prior to onset of symptoms; therefore, we concluded that these incidence data represent malaria transmission in the valley. Results of the study indicate that aerial spraying of ULV malathion can interrupt epidemic transmission of P. falciparum malaria by a susceptible vector.", "contents": "A prospective study of the effects of ultralow volume (ULV) aerial application of malathion on epidemic Plasmodium falciparum malaria. IV. Epidemiologic aspects. In the Miragoane Valley of Haiti a consistent pattern in the incidence of Plasmodium falciparum malaria over a 10-year period made it possible to predict an annual outbreak and perform a prospective study to test the effects of aerial ultralow volume (ULV) malathion on epidemic levels of this disease. At the end of October 1972, after epidemic levels (100 cases/month/10,000 population) had been reached, spray operations were begun. The first spray cycle produced a sharp and immediate drop in populations of the vector Anopheles albimanus, followed 4 weeks later by a decrease in the incidence of malaria throughout the valley. Although the incidence of malaria was similar in sprayed and unsprayed areas prior to the effect of ULV malathion (176.1 and 198.7 cases/month/10,000 population, respectively), it was significantly different during the subsequent 3 months (16.8 cases/month/10,000 population in sprayed areas and 65.4 in unsprayed; p less than 0.001). Travel histories indicated that only 4% of all cases had spent a night away from home during the 4 weeks prior to onset of symptoms; therefore, we concluded that these incidence data represent malaria transmission in the valley. Results of the study indicate that aerial spraying of ULV malathion can interrupt epidemic transmission of P. falciparum malaria by a susceptible vector."}
{"id": "PMID:1091171", "title": "Attempted prevention of diarrheal disease in Apache children with a non-absorbable broad-spectrum antimicrobial.", "content": "A double-blind study was conducted to test the prophylactic effect of a non-absorbable broad-spectrum antimicrobial (oral colistin sulfate) against acute diarrhea in Apache children. Children 1 to 6 months old had over twice the morbidity from diarrhea if assigned to the antimicrobial group as compared to placebo, while the toddler group (7-30 months) taking the antimicrobial had somewhat less diarrhea. Enteropathogenic E. coli were significantly more often isolated from the antimicrobial group (but only in well children). No special effect on the children's growth by the antimicrobial was discerned.", "contents": "Attempted prevention of diarrheal disease in Apache children with a non-absorbable broad-spectrum antimicrobial. A double-blind study was conducted to test the prophylactic effect of a non-absorbable broad-spectrum antimicrobial (oral colistin sulfate) against acute diarrhea in Apache children. Children 1 to 6 months old had over twice the morbidity from diarrhea if assigned to the antimicrobial group as compared to placebo, while the toddler group (7-30 months) taking the antimicrobial had somewhat less diarrhea. Enteropathogenic E. coli were significantly more often isolated from the antimicrobial group (but only in well children). No special effect on the children's growth by the antimicrobial was discerned."}
{"id": "PMID:1091172", "title": "Antimalarial effects of clindamycin in man.", "content": "Clindamycin 450 mg every 8 hours for 3 days cured three non-immune patients of falciparum malaria, although the response was slow. The addition of quinine to this regimen provided an accelerated response and cured 3 of 5 other patients. Single doses of clindamycin given daily for 3 days, with or without quinine, cured 1 of 3 patients. Gastric intolerance to the drugs, probably accentuated by the clinical condition, was pronounced in some cases, the course of treatment being interrupted in three patients for this reason. Chesson strain Plasmodium vivax relapses in man were not inhibited by ingestion of 450 mg of clindamycin every 6 hours for 14 days.", "contents": "Antimalarial effects of clindamycin in man. Clindamycin 450 mg every 8 hours for 3 days cured three non-immune patients of falciparum malaria, although the response was slow. The addition of quinine to this regimen provided an accelerated response and cured 3 of 5 other patients. Single doses of clindamycin given daily for 3 days, with or without quinine, cured 1 of 3 patients. Gastric intolerance to the drugs, probably accentuated by the clinical condition, was pronounced in some cases, the course of treatment being interrupted in three patients for this reason. Chesson strain Plasmodium vivax relapses in man were not inhibited by ingestion of 450 mg of clindamycin every 6 hours for 14 days."}
{"id": "PMID:1091174", "title": "Gastrointestinal and hepatic complications affecting patients with renal allografts.", "content": "Of 126 renal allograft recipients, 34 were found to have gastrointestinal and hepatic complications. In order of frequency, these included: mild liver dysfunction, severe hepatitis usually associated with cytomegalovirus infection, peptic ulceration complicated by bleeding, intestinal obstruction, and pancreatitis. These complications did not appear to influence the long-term survival or function of the renal allograft, but proved to be fatal when massive infection of cytomegalovirus affected the gastrointestinal tract and especially the liver. Gastrointestinal and pancreatic complications occurring in renal allograft recipients can be managed in the same manner as in patients who are not receiving immunosuppression. When surgical intervention is required, it should be performed promptly. The fact that these patients are receiving immunosuppressive therapy should not be a contraindication to early surgical intervention. When the presence of ulcerative lesions of the gastrointestinal mucosa, pancreatitis, or hepatitis is confirmed, the possibility of these lesions being caused by viral agents, especially cytomegalovirus, should be considered and attempts to confirm this diagnosis should be made. If cytomegalovirus infection is confirmed and the patient is experiencing rejection of the allograft, careful consideration should be given to immediate discontinuation of immunosuppressive therapy followed by removal of the renal allograft. In this way the relentless and fatal course of the cytomegalovirus infection seen in some of the patients reported in this study may be avoided.", "contents": "Gastrointestinal and hepatic complications affecting patients with renal allografts. Of 126 renal allograft recipients, 34 were found to have gastrointestinal and hepatic complications. In order of frequency, these included: mild liver dysfunction, severe hepatitis usually associated with cytomegalovirus infection, peptic ulceration complicated by bleeding, intestinal obstruction, and pancreatitis. These complications did not appear to influence the long-term survival or function of the renal allograft, but proved to be fatal when massive infection of cytomegalovirus affected the gastrointestinal tract and especially the liver. Gastrointestinal and pancreatic complications occurring in renal allograft recipients can be managed in the same manner as in patients who are not receiving immunosuppression. When surgical intervention is required, it should be performed promptly. The fact that these patients are receiving immunosuppressive therapy should not be a contraindication to early surgical intervention. When the presence of ulcerative lesions of the gastrointestinal mucosa, pancreatitis, or hepatitis is confirmed, the possibility of these lesions being caused by viral agents, especially cytomegalovirus, should be considered and attempts to confirm this diagnosis should be made. If cytomegalovirus infection is confirmed and the patient is experiencing rejection of the allograft, careful consideration should be given to immediate discontinuation of immunosuppressive therapy followed by removal of the renal allograft. In this way the relentless and fatal course of the cytomegalovirus infection seen in some of the patients reported in this study may be avoided."}
{"id": "PMID:1091175", "title": "Hereditary polypoid diseases of the gastrointestinal tract: a working classification.", "content": "Almost all published cases of hereditary intestinal polypoid diseases can be meaningfully classified into a relatively few distinct syndromes including familial polyposis of the colon, Peutz-Jeghers syndrome, and juvenile polyposis. Familial polyposis is characterized by the development of numerous adenomatous polyps of the colon and subsequent development of colorectal carcinoma in nearly all patients. Extracolonic manifestations are common but do not influence the premalignant nature of this syndrome. Peutz-Jeghers syndrome is identifiable by a combination of circumoral melanin pigmentation and hamartomatous polyps. These polypoid lesions have an unusually wide distribution and may occur in the respiratory, gastrointestinal, or genitourinary tract. There is a small but definite increased incidence of gastrointestinal cancer in these patients. Juvenile polyposis presents a more variable spectrum. In one form there is extensive intestinal involvement leading to diarrhea, inanition, and increased susceptibility to infection. Another form is limited to the colon and easily confused with familial polyposis. With the third form, there is involvement of the stomach, intestines, and colon, which makes it easily mistaken for the Peutz-Jeghers syndrome.", "contents": "Hereditary polypoid diseases of the gastrointestinal tract: a working classification. Almost all published cases of hereditary intestinal polypoid diseases can be meaningfully classified into a relatively few distinct syndromes including familial polyposis of the colon, Peutz-Jeghers syndrome, and juvenile polyposis. Familial polyposis is characterized by the development of numerous adenomatous polyps of the colon and subsequent development of colorectal carcinoma in nearly all patients. Extracolonic manifestations are common but do not influence the premalignant nature of this syndrome. Peutz-Jeghers syndrome is identifiable by a combination of circumoral melanin pigmentation and hamartomatous polyps. These polypoid lesions have an unusually wide distribution and may occur in the respiratory, gastrointestinal, or genitourinary tract. There is a small but definite increased incidence of gastrointestinal cancer in these patients. Juvenile polyposis presents a more variable spectrum. In one form there is extensive intestinal involvement leading to diarrhea, inanition, and increased susceptibility to infection. Another form is limited to the colon and easily confused with familial polyposis. With the third form, there is involvement of the stomach, intestines, and colon, which makes it easily mistaken for the Peutz-Jeghers syndrome."}
{"id": "PMID:1091176", "title": "Silastic T tube splints for biliary repair.", "content": "Silastic rubber T tubes have been compared with latex rubber T tubes in the biliary system of dogs. It was found that Silastic rubber T tubes evoked a significant foreign body reaction within the biliary system, producing bile salt precipitation and debris. Alarmingly, in these studies in dogs, Silastic rubber T tubes did not cause sufficient intraperitoneal adhesions around the tube to form an intraperitoneal tract walling off the tube from the general peritoneal cavity. Thus, when the Silastic rubber T tube was removed, bile leakage into the peritoneal cavity occurred. Based on studies in dogs, Silastic rubber T tubes should not be used in the clinical situation in which the T tube would be removed postoperatively.", "contents": "Silastic T tube splints for biliary repair. Silastic rubber T tubes have been compared with latex rubber T tubes in the biliary system of dogs. It was found that Silastic rubber T tubes evoked a significant foreign body reaction within the biliary system, producing bile salt precipitation and debris. Alarmingly, in these studies in dogs, Silastic rubber T tubes did not cause sufficient intraperitoneal adhesions around the tube to form an intraperitoneal tract walling off the tube from the general peritoneal cavity. Thus, when the Silastic rubber T tube was removed, bile leakage into the peritoneal cavity occurred. Based on studies in dogs, Silastic rubber T tubes should not be used in the clinical situation in which the T tube would be removed postoperatively."}
{"id": "PMID:1091177", "title": "Reduction of acute tubular necrosis (ATN) by furosemide and steroids in cadaveric kidney recovery.", "content": "One hundred fifty human cadaver kidneys have been preserved by a combination of topical hypothermia and pulsatile perfusion with cryoprecipitated plasma in our laboratory. Post-transplant acute renal failure was reduced from 58 to 21 per cent in those kidneys protected with furosemide and methylprednisolone.", "contents": "Reduction of acute tubular necrosis (ATN) by furosemide and steroids in cadaveric kidney recovery. One hundred fifty human cadaver kidneys have been preserved by a combination of topical hypothermia and pulsatile perfusion with cryoprecipitated plasma in our laboratory. Post-transplant acute renal failure was reduced from 58 to 21 per cent in those kidneys protected with furosemide and methylprednisolone."}
{"id": "PMID:1091178", "title": "Calculi complicating a renal transplant.", "content": "Four months after a cadaver kidney transplant, kidney stones were found in the renal allograft. Three major predisposing causes of nephrolithiasis were found in the patient, including hyperparathyroidism, renal tubular acidosis, and urinary tract infection. Hypercalcemia was corrected by parathyroidectomy. During the subsequent three years there was no enlargement of the renal stones and adequate kidney function was maintained. Renal tubular acidosis was not severe and seemed to be related to chronic rejection. Urinary tract infection was readily corrected with antibiotics and did not recur after the immediate post-transplant period. Surgical therapy for nephrolithiasis involving a kidney allograft was defferred since urinary flow was not obstructed. This course of management is recommended for use in patients with calculi complicating renal transplantation.", "contents": "Calculi complicating a renal transplant. Four months after a cadaver kidney transplant, kidney stones were found in the renal allograft. Three major predisposing causes of nephrolithiasis were found in the patient, including hyperparathyroidism, renal tubular acidosis, and urinary tract infection. Hypercalcemia was corrected by parathyroidectomy. During the subsequent three years there was no enlargement of the renal stones and adequate kidney function was maintained. Renal tubular acidosis was not severe and seemed to be related to chronic rejection. Urinary tract infection was readily corrected with antibiotics and did not recur after the immediate post-transplant period. Surgical therapy for nephrolithiasis involving a kidney allograft was defferred since urinary flow was not obstructed. This course of management is recommended for use in patients with calculi complicating renal transplantation."}
{"id": "PMID:1091179", "title": "Skin closure using a new skin clip.", "content": "A new skin clip that allows rapid skin closure with minimal trauma to the skin has been devised. The clip can be used in most conventional incisions on the neck, extremities, thorax, and abdomen. It cannot be used where skin is tightly adherent, as in the scalp, palm, and soles of the feet. Closure with this skin clip has proved to be dependable and to provide an aesthetic hairline scar.", "contents": "Skin closure using a new skin clip. A new skin clip that allows rapid skin closure with minimal trauma to the skin has been devised. The clip can be used in most conventional incisions on the neck, extremities, thorax, and abdomen. It cannot be used where skin is tightly adherent, as in the scalp, palm, and soles of the feet. Closure with this skin clip has proved to be dependable and to provide an aesthetic hairline scar."}
{"id": "PMID:1091180", "title": "[Further investigations for the demonstration of a specific microorganism in sympathetik ophthalmia].", "content": "19 Following the inoculation of tissue culture cells with material obtained from 3 human eyes suffering from sympathetic ophthalmia (2 sympathizing = sy-l, 1 sympathized = sy-2), no growth of bacteria was observed. On the other hand, changes occurred such as are typical for a massive infection with mycoplasmas. The morphology of these microbes corresponds, down to the details, with micro-organisms which the author has frequently demonstrated and illustrated since 1948 in human, chicken and monkey eyes suffering from sympathetic ophthalmia. 2. With the cultures of mycoplasmas obtained from the eyes of human patients suffering from sympathetic ophthalmia, it was possible to produce the same symptoms in chickens as were described by the author in 1950 in sympathizing and sympathized human eyes, namely: torpid uveitis and papillitis, which dragged on for months, and affected not only the inoculated right eye, but also, after 3 weeks and more, the untouched left eye. 3. Identical phenomena involving both eyes have been observed in chickens, the right eyes of which were inoculated with a strain of mycoplasma hominis orale 1 cultivated from other material in the Institut f\u00fcr medizinische Mikrobiologie der Universit\u00e4t Mainz (Prof. Bredt) and given to the author. A chronic intraocular inflammation, which persisted for several months--up to 6 months and more--developed not only in the inoculated right eye of the animals, but also, after an interval of several weeks, in the untouched left eye. 4. Viewed critically and with due care, these findings suggest that mycoplasmas might well play a role in triggering off sympathetic ophthalmia. 5. The results reported on here indicate that intraocular inoculation with mycoplasmas is of importance both in microbiology and in general medicine.", "contents": "[Further investigations for the demonstration of a specific microorganism in sympathetik ophthalmia]. 19 Following the inoculation of tissue culture cells with material obtained from 3 human eyes suffering from sympathetic ophthalmia (2 sympathizing = sy-l, 1 sympathized = sy-2), no growth of bacteria was observed. On the other hand, changes occurred such as are typical for a massive infection with mycoplasmas. The morphology of these microbes corresponds, down to the details, with micro-organisms which the author has frequently demonstrated and illustrated since 1948 in human, chicken and monkey eyes suffering from sympathetic ophthalmia. 2. With the cultures of mycoplasmas obtained from the eyes of human patients suffering from sympathetic ophthalmia, it was possible to produce the same symptoms in chickens as were described by the author in 1950 in sympathizing and sympathized human eyes, namely: torpid uveitis and papillitis, which dragged on for months, and affected not only the inoculated right eye, but also, after 3 weeks and more, the untouched left eye. 3. Identical phenomena involving both eyes have been observed in chickens, the right eyes of which were inoculated with a strain of mycoplasma hominis orale 1 cultivated from other material in the Institut f\u00fcr medizinische Mikrobiologie der Universit\u00e4t Mainz (Prof. Bredt) and given to the author. A chronic intraocular inflammation, which persisted for several months--up to 6 months and more--developed not only in the inoculated right eye of the animals, but also, after an interval of several weeks, in the untouched left eye. 4. Viewed critically and with due care, these findings suggest that mycoplasmas might well play a role in triggering off sympathetic ophthalmia. 5. The results reported on here indicate that intraocular inoculation with mycoplasmas is of importance both in microbiology and in general medicine."}
{"id": "PMID:1091181", "title": "The immune system of the nine-banded armadillo (Dasypus novemcinctus, Linn).", "content": "The armadillo is rapidly emerging as an animal for biomedical research. A morphological study of the immune system of the armadillo was undertaken to try to demonstrate a possible defect in the morphogenesis of the lymphoid organs that could account for their susceptibility to leprosy. Blood from 20 domesticated adult armadillos revealed lymphocytes predominated among circulating leukocytes in the majority of animals, whereas in wild animals neutrophils predominated. Necropsies revealed well-developed thymus glands and the thymus-dependent areas in lymph nodes and spleens contained abundant lymphoid cells. Hence, we conclude that the susceptibility of the armadillo to leprosy cannot be explained on a morphological basis.", "contents": "The immune system of the nine-banded armadillo (Dasypus novemcinctus, Linn). The armadillo is rapidly emerging as an animal for biomedical research. A morphological study of the immune system of the armadillo was undertaken to try to demonstrate a possible defect in the morphogenesis of the lymphoid organs that could account for their susceptibility to leprosy. Blood from 20 domesticated adult armadillos revealed lymphocytes predominated among circulating leukocytes in the majority of animals, whereas in wild animals neutrophils predominated. Necropsies revealed well-developed thymus glands and the thymus-dependent areas in lymph nodes and spleens contained abundant lymphoid cells. Hence, we conclude that the susceptibility of the armadillo to leprosy cannot be explained on a morphological basis."}
{"id": "PMID:1091183", "title": "The use of haloperidol for treatment of postoperative nausea and vomiting--a double-blind placebo-controlled trial.", "content": "Sixty-two postoperative patients were admitted to a double-blind study to compare the therapeutic effectiveness of a single intramuscular injection of 1 mg of haloperidol with that of a placebo for the relief of vomiting and nausea following surgical procedures. Significantly fewer patients continued to vomit or experience nausea in the haloperidol-treatment group than in the placebo-treatment group. No statistically or clinically significant change in vital signs occurred in the haloperidol-treatment group. No serious side effect occurred in either group.", "contents": "The use of haloperidol for treatment of postoperative nausea and vomiting--a double-blind placebo-controlled trial. Sixty-two postoperative patients were admitted to a double-blind study to compare the therapeutic effectiveness of a single intramuscular injection of 1 mg of haloperidol with that of a placebo for the relief of vomiting and nausea following surgical procedures. Significantly fewer patients continued to vomit or experience nausea in the haloperidol-treatment group than in the placebo-treatment group. No statistically or clinically significant change in vital signs occurred in the haloperidol-treatment group. No serious side effect occurred in either group."}
{"id": "PMID:1091184", "title": "A computer-assisted instruction course in the diagnosis and treatment of respiratory diseases.", "content": "A computer-assisted simulation of the 'chart method' of teaching has been developed and was used to provide instruction in clinical decision-making in the diagonosis and treatment of pulmonary diseases. The course requires a student to reach and to defend a diagonisis and to provide appropriate treatment for each of 10 simulated cases. Evaluation of performance and immediate feedback and correction of errors of commission and ommission are an integral part of the course. The course provides a model for development of additional programs in other subject areas.", "contents": "A computer-assisted instruction course in the diagnosis and treatment of respiratory diseases. A computer-assisted simulation of the 'chart method' of teaching has been developed and was used to provide instruction in clinical decision-making in the diagonosis and treatment of pulmonary diseases. The course requires a student to reach and to defend a diagonisis and to provide appropriate treatment for each of 10 simulated cases. Evaluation of performance and immediate feedback and correction of errors of commission and ommission are an integral part of the course. The course provides a model for development of additional programs in other subject areas."}
{"id": "PMID:1091185", "title": "Effect of prostaglandins F2 alpha and E2 on airway conductance in healthy subjects and asthmatic patients.", "content": "Effects of aerosol inhalation of prostaglandins F2alpha and E2 on specific airway conductance were studied in 12 healthy subjects and 17 patients with bronchial asthma. Approximately 50 per cent reduction of specific airway conductance was obtained with 250 to 1000 mug of prostaglandin F2alpha in healthy subjects and 4 to 512 ng of prostaglandin F2alpha in asthmatics. In healthy subjects, repeated administration of prostaglandin F2alpha within 1 session elicited nearly identical changes specific airway conductance, whereas a degree of tachyphylaxis possible existed in asthmatics. The sensitivity to prostaglandin F2alpha during a 17 month period remained quite constand; test-retest responses did not differ significantly. Pretreatment with 1 mg of atropine did not modify the effect of prostaglandin F2alpha in health subjects. In contrast. bronchoconstriction in asthmatics was diminished 1 and 5 but not 10 minutes after inhalation prostaglandin F2alpha. Prostaglandin E2 was a bronchodilator in healthy subjects but caused either dilation or constriction (the direction of the change in specific airway conductance being consistent for each subject) in asthamatics.", "contents": "Effect of prostaglandins F2 alpha and E2 on airway conductance in healthy subjects and asthmatic patients. Effects of aerosol inhalation of prostaglandins F2alpha and E2 on specific airway conductance were studied in 12 healthy subjects and 17 patients with bronchial asthma. Approximately 50 per cent reduction of specific airway conductance was obtained with 250 to 1000 mug of prostaglandin F2alpha in healthy subjects and 4 to 512 ng of prostaglandin F2alpha in asthmatics. In healthy subjects, repeated administration of prostaglandin F2alpha within 1 session elicited nearly identical changes specific airway conductance, whereas a degree of tachyphylaxis possible existed in asthmatics. The sensitivity to prostaglandin F2alpha during a 17 month period remained quite constand; test-retest responses did not differ significantly. Pretreatment with 1 mg of atropine did not modify the effect of prostaglandin F2alpha in health subjects. In contrast. bronchoconstriction in asthmatics was diminished 1 and 5 but not 10 minutes after inhalation prostaglandin F2alpha. Prostaglandin E2 was a bronchodilator in healthy subjects but caused either dilation or constriction (the direction of the change in specific airway conductance being consistent for each subject) in asthamatics."}
{"id": "PMID:1091187", "title": "Subfascial ligation (Linton operation) of the perforating leg veins to treat post-thrombophlebitic syndrome.", "content": "A series of 71 subfascial ligations of the perforating veins of the lower leg is discussed, and the technique of the operation outlined. Forty-four per cent of the limbs we treated developed a postoperative slough, and 25 per cent required skin grafting. There was an ulcer recurrence rate of 33.8 per cent in our series, but these ulcers usually promptly healed with compression bandaging. In 13 per cent of the limbs, further operative procedures were required. There was no operative mortality in this series.", "contents": "Subfascial ligation (Linton operation) of the perforating leg veins to treat post-thrombophlebitic syndrome. A series of 71 subfascial ligations of the perforating veins of the lower leg is discussed, and the technique of the operation outlined. Forty-four per cent of the limbs we treated developed a postoperative slough, and 25 per cent required skin grafting. There was an ulcer recurrence rate of 33.8 per cent in our series, but these ulcers usually promptly healed with compression bandaging. In 13 per cent of the limbs, further operative procedures were required. There was no operative mortality in this series."}
{"id": "PMID:1091188", "title": "Decreasing reliability of acid-fast smear techniques for detection of tuberculosis.", "content": "The ability of any screening test to correctly identify diseased patients is directly related to the prevalence of the disease in question. The continuing use of smears for the detection of acid-fast bacilli when the prevalence of tuberculosis is declining will produce increasing numbers of false-positive results. To determine the usefulness of smear techniques, we carried out a retrospective study of approximately 10 000 consecutive smears and cultures. Positive smears were classified as true- or false-positive on the basis of whether the corresponding cultures grew mycobacteria. The overall incidence of false-positive smears was 0.7%; however, the ratio of the number of false-positive to total positive smear was about 0.5. Thus, for each true-positive result, one false-positive result was obtained. Patients in the true-positive group had clinical and radiological evidence to support the diagnosis of tuberculosis, while those in the false-positive group had new findings of the disease. These data suggest that the smear is a poor screening technique in a population where the prevalence of tuberculosis is low.", "contents": "Decreasing reliability of acid-fast smear techniques for detection of tuberculosis. The ability of any screening test to correctly identify diseased patients is directly related to the prevalence of the disease in question. The continuing use of smears for the detection of acid-fast bacilli when the prevalence of tuberculosis is declining will produce increasing numbers of false-positive results. To determine the usefulness of smear techniques, we carried out a retrospective study of approximately 10 000 consecutive smears and cultures. Positive smears were classified as true- or false-positive on the basis of whether the corresponding cultures grew mycobacteria. The overall incidence of false-positive smears was 0.7%; however, the ratio of the number of false-positive to total positive smear was about 0.5. Thus, for each true-positive result, one false-positive result was obtained. Patients in the true-positive group had clinical and radiological evidence to support the diagnosis of tuberculosis, while those in the false-positive group had new findings of the disease. These data suggest that the smear is a poor screening technique in a population where the prevalence of tuberculosis is low."}
{"id": "PMID:1091189", "title": "Hyperglucagonemia in uremia: reversal by renal transplantation.", "content": "Chronic renal failure in man is associated with hyperglucagonemia that is not corrected by hemodialysis. Plasma glucagon concentrations were measured in nine patients before and after renal transplantation. Mean plasma glucagon concentration in eight patients with chronic renal failure before transplantation was 295 plus or minus 171 pg/ml (plus or minus SD). After successful transplantation, mean plasma glucagon concentration fell to 134 plus or minus 81 pg/ml (plus or minus SD) (P less than 0.001). Plasma glucagon concentration remained elevated in an additional patient who received a cadaveric graft that never functioned. Immunologic rejection of transplanted kidneys was associated with a dramatic increase of plasma glucagon concentration.", "contents": "Hyperglucagonemia in uremia: reversal by renal transplantation. Chronic renal failure in man is associated with hyperglucagonemia that is not corrected by hemodialysis. Plasma glucagon concentrations were measured in nine patients before and after renal transplantation. Mean plasma glucagon concentration in eight patients with chronic renal failure before transplantation was 295 plus or minus 171 pg/ml (plus or minus SD). After successful transplantation, mean plasma glucagon concentration fell to 134 plus or minus 81 pg/ml (plus or minus SD) (P less than 0.001). Plasma glucagon concentration remained elevated in an additional patient who received a cadaveric graft that never functioned. Immunologic rejection of transplanted kidneys was associated with a dramatic increase of plasma glucagon concentration."}
{"id": "PMID:1091194", "title": "A review on neurosecretory granules: their contents and mechanisms of release.", "content": "The available evidence suggests that hormones and neurophysins are associated exclusively with the neurosecretory granules, each of which contains approximately 6 times 10-4 molecules of each. Hormones and carrier proteins are complexed within the granules and the complexes are densely packed. The processes that keep the intragranular space in osmotic equilibrium with the axoplasm require further study. Freeze-fracture data, as well as studies in which histochemical methods for the detection of glycoproteins were used, suggest that the intragranular aspect of the granule membrane mostly resembles the extracellular half of the plasma membrane; on the other hand, the cytoplasmic aspects of plasma and granule membrane have similar characteristics, which may be important in permitting membrane fusion to take place prior to secretion. Little is known about the molecular species involved in this interaction between granule and plasma membrane, except that calcium is a cofactor in this process. Release is triggered in vivo by propagated action potentials which cause an influx of calcium into the secretory endings. Newly formed granules, and other granules located at the periphery of the endings are preferentially released. Irrespective of the type of stimulation of secretion, release involves the diffusion into the extracellular space of granule core constituents. The best evidence so far in support of this view comes from ultrastructural studies showing images of exocytosis, as well as from biochemical studies demonstrating that hormones and carrier proteins are secreted concomitantly in a great variety of experimental or clinical conditions, without an associated release of granule membrane constituents or of enzymes of cytoplasmic origin. Recovery mechanisms following secretion require new synthesis of granule constituents and restoration of the resting internal concentrations of potassium, sodium, and calcium. Membrane surface area is restored following exocytosis by compensatory endocytosis which involves indiscriminate uptake of extracellular medium into the secretory axon terminals. While much progress has been made in research on the cellular and subcellular processes that take place in neurons which produce, store, and secrete neurohypophyseal hormones and their carrier proteins, neurophysins, many pressing questions remain to be answered. New developments, such as organ culture of supraoptic nuclei94-96 and the recent isolation of a clone of mouse hypothalamic cells capable of synthesizing vasopressin and neurophysin,97 will hopefully allow some of these problems to be solved in the future.", "contents": "A review on neurosecretory granules: their contents and mechanisms of release. The available evidence suggests that hormones and neurophysins are associated exclusively with the neurosecretory granules, each of which contains approximately 6 times 10-4 molecules of each. Hormones and carrier proteins are complexed within the granules and the complexes are densely packed. The processes that keep the intragranular space in osmotic equilibrium with the axoplasm require further study. Freeze-fracture data, as well as studies in which histochemical methods for the detection of glycoproteins were used, suggest that the intragranular aspect of the granule membrane mostly resembles the extracellular half of the plasma membrane; on the other hand, the cytoplasmic aspects of plasma and granule membrane have similar characteristics, which may be important in permitting membrane fusion to take place prior to secretion. Little is known about the molecular species involved in this interaction between granule and plasma membrane, except that calcium is a cofactor in this process. Release is triggered in vivo by propagated action potentials which cause an influx of calcium into the secretory endings. Newly formed granules, and other granules located at the periphery of the endings are preferentially released. Irrespective of the type of stimulation of secretion, release involves the diffusion into the extracellular space of granule core constituents. The best evidence so far in support of this view comes from ultrastructural studies showing images of exocytosis, as well as from biochemical studies demonstrating that hormones and carrier proteins are secreted concomitantly in a great variety of experimental or clinical conditions, without an associated release of granule membrane constituents or of enzymes of cytoplasmic origin. Recovery mechanisms following secretion require new synthesis of granule constituents and restoration of the resting internal concentrations of potassium, sodium, and calcium. Membrane surface area is restored following exocytosis by compensatory endocytosis which involves indiscriminate uptake of extracellular medium into the secretory axon terminals. While much progress has been made in research on the cellular and subcellular processes that take place in neurons which produce, store, and secrete neurohypophyseal hormones and their carrier proteins, neurophysins, many pressing questions remain to be answered. New developments, such as organ culture of supraoptic nuclei94-96 and the recent isolation of a clone of mouse hypothalamic cells capable of synthesizing vasopressin and neurophysin,97 will hopefully allow some of these problems to be solved in the future."}
{"id": "PMID:1091202", "title": "[Possible mechanisms of the rifampicin suppression of the conjugation transfer of R-factors in Escherichia coli].", "content": "The studies on the effect of a number of antibiotics on transfer of R-factors with the use of a kinetic conjugation system showed that rifampicin inhibited the transfer of various R-factors independent of the microbial host strain and R-factor type (the inhibition coefficient of I infinity equals 7.5-16.8). The early conjugation stages preceding the transfer of R-factor DNA were most sensitive to the antibiotic effect. Decreased number of R+ conjugants observed on the donor strain growth in the presence of rifampicin were due rather to selection of spontaneous R- variants with the antibiotic found in our experiments than to its direct effect on the donor competence of R+ cells. It was also found that exposure of E. coli strains to rifampicin for 30 minutes increased their sensitivity to chloramphenicol and tetracycline, this being more pronounced in the cells which not long ago acquired the R-factor. The mechanism of the phenomenon is not clear yet. It was shown to be not associated with the cell permeability impairement under the effect of rifampicin. It was noted that rifampicin combination with neomycin chloramphenicol, tetracycline or streptomycin had a synergistic effect on the strains of E. coli.", "contents": "[Possible mechanisms of the rifampicin suppression of the conjugation transfer of R-factors in Escherichia coli]. The studies on the effect of a number of antibiotics on transfer of R-factors with the use of a kinetic conjugation system showed that rifampicin inhibited the transfer of various R-factors independent of the microbial host strain and R-factor type (the inhibition coefficient of I infinity equals 7.5-16.8). The early conjugation stages preceding the transfer of R-factor DNA were most sensitive to the antibiotic effect. Decreased number of R+ conjugants observed on the donor strain growth in the presence of rifampicin were due rather to selection of spontaneous R- variants with the antibiotic found in our experiments than to its direct effect on the donor competence of R+ cells. It was also found that exposure of E. coli strains to rifampicin for 30 minutes increased their sensitivity to chloramphenicol and tetracycline, this being more pronounced in the cells which not long ago acquired the R-factor. The mechanism of the phenomenon is not clear yet. It was shown to be not associated with the cell permeability impairement under the effect of rifampicin. It was noted that rifampicin combination with neomycin chloramphenicol, tetracycline or streptomycin had a synergistic effect on the strains of E. coli."}
{"id": "PMID:1091203", "title": "[Recipient capacity of B. alcalescens strains in the conjugation transfer of R-factors to them for E. coli strains].", "content": "Unlike many enterobacteria the strains of B. alkalescens isolated from patients with the intestine dysfunction and healthy persons preserved sensitivity to the antibiotics used in the medical practice. This served as a basis for the study of the recipient competence for reception of R-factors from E. coli by 6 strains of the alkali producer. The strains of E. coli with the derepressive R-factors J 5-3 R1, J 5-3 R64 and CSH-2 R1-19 were introduced into the conjugation mixture as the donors. Only I strain of E. coli, i.e. J 5-3 R1 with multiple antibiotic resistance proved to be a competent donor. A possibility of transfering on1 I determinant of resistance (to neomycin) to 3 out of 6 strains of B. alkalescens at a low rate of10-7 and 10-9 was shown. The R+ conjugants appeared to be immune to further reception of R-factors of both the competent and the incompetent donors. The low recipient capacity of the strains of B. alkalescens must, to some extent promote circulation mainly of antibiotic sensitive strains of the microbes under natural conditions.", "contents": "[Recipient capacity of B. alcalescens strains in the conjugation transfer of R-factors to them for E. coli strains]. Unlike many enterobacteria the strains of B. alkalescens isolated from patients with the intestine dysfunction and healthy persons preserved sensitivity to the antibiotics used in the medical practice. This served as a basis for the study of the recipient competence for reception of R-factors from E. coli by 6 strains of the alkali producer. The strains of E. coli with the derepressive R-factors J 5-3 R1, J 5-3 R64 and CSH-2 R1-19 were introduced into the conjugation mixture as the donors. Only I strain of E. coli, i.e. J 5-3 R1 with multiple antibiotic resistance proved to be a competent donor. A possibility of transfering on1 I determinant of resistance (to neomycin) to 3 out of 6 strains of B. alkalescens at a low rate of10-7 and 10-9 was shown. The R+ conjugants appeared to be immune to further reception of R-factors of both the competent and the incompetent donors. The low recipient capacity of the strains of B. alkalescens must, to some extent promote circulation mainly of antibiotic sensitive strains of the microbes under natural conditions."}
{"id": "PMID:1091204", "title": "[Bacteriocinogeny in Yersinia enterocolitica].", "content": "Bacteriocinogeny was found in 19.8 per cent of Y. enterocolitica strains tested. It was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. Bacteriocinogeny was found among the collection strains isolated in the South Primorye and not among the strains isolated abroad. Some cultures of Y. enterocolitica isolated abroad and in the South Primorye were used as indicator strains for revealing enterocoliticacinogeny. Enterocoliticacine inhibited growth only of some strains of Y. enterocolitica and had no inhibitory effect on growth of Coli bacteria, plague and pseudotuberculosis microbes. The latter 3 bacterial species did not inhibit growth of the causative agent of gastric yearsiniosis, which may be of some diagnostic importance. The diverse spectrum of enterocoliticacine and the character of inhibition of sensitive culture growth are indicative of the presence of several types of bacteriocines in Y. enterocolitica.", "contents": "[Bacteriocinogeny in Yersinia enterocolitica]. Bacteriocinogeny was found in 19.8 per cent of Y. enterocolitica strains tested. It was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. Bacteriocinogeny was found among the collection strains isolated in the South Primorye and not among the strains isolated abroad. Some cultures of Y. enterocolitica isolated abroad and in the South Primorye were used as indicator strains for revealing enterocoliticacinogeny. Enterocoliticacine inhibited growth only of some strains of Y. enterocolitica and had no inhibitory effect on growth of Coli bacteria, plague and pseudotuberculosis microbes. The latter 3 bacterial species did not inhibit growth of the causative agent of gastric yearsiniosis, which may be of some diagnostic importance. The diverse spectrum of enterocoliticacine and the character of inhibition of sensitive culture growth are indicative of the presence of several types of bacteriocines in Y. enterocolitica."}
{"id": "PMID:1091205", "title": "[Effectiveness of prodigiozan in the therapy of chronic nonspecific diseases of the lungs].", "content": "The efficiency of the Soviet prodigiozan in therapy of chronic non-specific diseases of the lungs (18 cases with bronchial asthma and 26 cases with chronic pneumonia of the 1st and 2nd stages) in the phase of exacerbation was studied. The results of the treatment were estimated by the clinico-roentgenological pictures. The effect of prodigiozan on breathing and the functional state of the adrenal gland was studied in particular. Satisfactory results were obtained in the patients suffering from chronic pneumonia of the 1st and 2nd stages without clinical signs of the bronchial tree affection and in the patients with infection-allergic forms of bronchial asthma. Modest results were observed in the patients suffering from chronic pneumonia of the 1st and 2nd stages with the bronchial tree affection, especially when exacerbated by the asthma syndrome. In some patients the results of the treatment with prodigiozan were not stable. Prodigiozan improved the bronchial permeability, stimulated the sympatho-adrenal system and had no effect on the adrenal cortex.", "contents": "[Effectiveness of prodigiozan in the therapy of chronic nonspecific diseases of the lungs]. The efficiency of the Soviet prodigiozan in therapy of chronic non-specific diseases of the lungs (18 cases with bronchial asthma and 26 cases with chronic pneumonia of the 1st and 2nd stages) in the phase of exacerbation was studied. The results of the treatment were estimated by the clinico-roentgenological pictures. The effect of prodigiozan on breathing and the functional state of the adrenal gland was studied in particular. Satisfactory results were obtained in the patients suffering from chronic pneumonia of the 1st and 2nd stages without clinical signs of the bronchial tree affection and in the patients with infection-allergic forms of bronchial asthma. Modest results were observed in the patients suffering from chronic pneumonia of the 1st and 2nd stages with the bronchial tree affection, especially when exacerbated by the asthma syndrome. In some patients the results of the treatment with prodigiozan were not stable. Prodigiozan improved the bronchial permeability, stimulated the sympatho-adrenal system and had no effect on the adrenal cortex."}
{"id": "PMID:1091206", "title": "[Dynamics of the antibiotic sensitivity of the staphylococci in suppurative, inflammatory foci in patients during the period of the hospital stay].", "content": "The dynamics of staphylococci sensitivity to antibiotics in purulent foci of 242 in patients was studied. Two criteria were used for estimation of the above process, i.e. (I) sensitivity to the antibiotics of the whole staphylococcal population isolated and (2) sensitivity to the antibiotics of separate strains isolated from the population. In 10 or more days the number of the in-patients with staphylococci resistant to streptomycin, monomycin or oxacillin in the purulent foci increased. The number of the in-patients with penicillin, neomycin or chloramphenicol resistant staphylocci was statistically doubtful. The average number of the antibiotic resistant microbial cells in the staphylococcal populations of the foci in the patients during their stay in the hospital increased 2-4 times.", "contents": "[Dynamics of the antibiotic sensitivity of the staphylococci in suppurative, inflammatory foci in patients during the period of the hospital stay]. The dynamics of staphylococci sensitivity to antibiotics in purulent foci of 242 in patients was studied. Two criteria were used for estimation of the above process, i.e. (I) sensitivity to the antibiotics of the whole staphylococcal population isolated and (2) sensitivity to the antibiotics of separate strains isolated from the population. In 10 or more days the number of the in-patients with staphylococci resistant to streptomycin, monomycin or oxacillin in the purulent foci increased. The number of the in-patients with penicillin, neomycin or chloramphenicol resistant staphylocci was statistically doubtful. The average number of the antibiotic resistant microbial cells in the staphylococcal populations of the foci in the patients during their stay in the hospital increased 2-4 times."}
{"id": "PMID:1091207", "title": "[Comparative clinical electroencephalographic study of cycloserine and terizidon tolerance].", "content": "The aim of the study was comparative investigation of cycloserin and terizidone tolerance by patients with the lung tuberculosis. The investigation procedure included parallel clinical observations and electroencephalographic studies (EEG) using light and sonic stimulation. Sixty six and 26 patients treated with cycloserin and terizidone respectively were observed. The results of the studies showed that by the mechanism of their action on the central nervous system terizidone and cycloserine were similar and increased the general reactivity of the brain. However, terizidone promoted an increase of the activating effect of the ascending part of the reticular formation of the brain stem to a lesser extent than cycloserine. Terizidone in a dose of 0.25 g 3 times a day was well tolerated by the patients with concomitant psycho-neurological impairements.", "contents": "[Comparative clinical electroencephalographic study of cycloserine and terizidon tolerance]. The aim of the study was comparative investigation of cycloserin and terizidone tolerance by patients with the lung tuberculosis. The investigation procedure included parallel clinical observations and electroencephalographic studies (EEG) using light and sonic stimulation. Sixty six and 26 patients treated with cycloserin and terizidone respectively were observed. The results of the studies showed that by the mechanism of their action on the central nervous system terizidone and cycloserine were similar and increased the general reactivity of the brain. However, terizidone promoted an increase of the activating effect of the ascending part of the reticular formation of the brain stem to a lesser extent than cycloserine. Terizidone in a dose of 0.25 g 3 times a day was well tolerated by the patients with concomitant psycho-neurological impairements."}
{"id": "PMID:1091213", "title": "Tumor angiogenic factor and human skin tumors.", "content": "A transparent acrylic hamster cheek-pouch chamber was used to investigate the elaboration of a tumor angiogenic factor (TAF) by human cutaneous neoplasms; direct tumor implantations, transfilter diffusion, and soluble tumor extracts were used in the study. A diffusible and filterable TAF was extracted from cutaneous tumors and produced distinctive patterns of sequential vasodilatation, tortuosity, and neovascular proliferation in the cheek-pouch membrane. Malignant human neoplasms (eg, melanoma, basal cell epithelioma, squamous cell carcinoma, lymphoma) produced striking neovascularization; vascular tumors (eg, Kaposi sarcoma, pyogenic granuloma, vascular histiocytoma) stimulated dramatic hyperemia and ectasia. Angiogenesis was conspicuously absent after implantation of control materials and nevoid or normal cutaneous components (with the exception of epidermis). Tumor angiogenic factor appears to induce direct stimulation of endothelial cell mitosis and may be essential for survival of nutritionally ravenous neoplastic tissues. The interference with TAF has therapeutic implications.", "contents": "Tumor angiogenic factor and human skin tumors. A transparent acrylic hamster cheek-pouch chamber was used to investigate the elaboration of a tumor angiogenic factor (TAF) by human cutaneous neoplasms; direct tumor implantations, transfilter diffusion, and soluble tumor extracts were used in the study. A diffusible and filterable TAF was extracted from cutaneous tumors and produced distinctive patterns of sequential vasodilatation, tortuosity, and neovascular proliferation in the cheek-pouch membrane. Malignant human neoplasms (eg, melanoma, basal cell epithelioma, squamous cell carcinoma, lymphoma) produced striking neovascularization; vascular tumors (eg, Kaposi sarcoma, pyogenic granuloma, vascular histiocytoma) stimulated dramatic hyperemia and ectasia. Angiogenesis was conspicuously absent after implantation of control materials and nevoid or normal cutaneous components (with the exception of epidermis). Tumor angiogenic factor appears to induce direct stimulation of endothelial cell mitosis and may be essential for survival of nutritionally ravenous neoplastic tissues. The interference with TAF has therapeutic implications."}
{"id": "PMID:1091214", "title": "Lupus erythematosus profundus following thrombocytopenic purpura.", "content": "A patient with thrombocytopenic purpura subsequently exhibited skin lesions of lupus erythematosus profundus, serositis, and serum antinuclear antibodies. Immunoglobulins and complement were found in the dermoepidermal junction of active skin lesions. Also of note was the presence of possible systemic lupus erythematosus in his mother.", "contents": "Lupus erythematosus profundus following thrombocytopenic purpura. A patient with thrombocytopenic purpura subsequently exhibited skin lesions of lupus erythematosus profundus, serositis, and serum antinuclear antibodies. Immunoglobulins and complement were found in the dermoepidermal junction of active skin lesions. Also of note was the presence of possible systemic lupus erythematosus in his mother."}
{"id": "PMID:1091215", "title": "Uses for immunofluorescence tests of skin and sera. Utilization of immunofluorescence in the diagnosis of bullous diseases, lupus erythematosus, and certain other dermatoses.", "content": "Sera and frozen biopsy specimens taken for immunofluorescence can be of diagnostic value in studies of vesiculobullous eruptions and connective tissue diseases. Appropriate handling of specimens is important. In cases of pemphigus and pemphigoid, findings of typical antibodies in sera and immunoglobulin deposits in skin sections serve to establish the diagnoses. Fluctuations in titers of pemphigus antibodies afford a prognistic guide. IgA deposits at the dermoepidermal junction in patients with vesiculobullous eruptions are diagnostic of dermatitis herpetiformis. Elevated titers of antinuclear antibodies, frequently with a peripheral pattern, and DNA antibodies as well as junctional deposits of immunoglobulins or complement or both in the apparently uninvolved skin are highly characteristic of systemic lupus erythematosus, more or less typical patterns of immunoglobulin and complement deposits in the lesion appear in discoid lupus erythematosus, porphyrias, and some other dermatoses.", "contents": "Uses for immunofluorescence tests of skin and sera. Utilization of immunofluorescence in the diagnosis of bullous diseases, lupus erythematosus, and certain other dermatoses. Sera and frozen biopsy specimens taken for immunofluorescence can be of diagnostic value in studies of vesiculobullous eruptions and connective tissue diseases. Appropriate handling of specimens is important. In cases of pemphigus and pemphigoid, findings of typical antibodies in sera and immunoglobulin deposits in skin sections serve to establish the diagnoses. Fluctuations in titers of pemphigus antibodies afford a prognistic guide. IgA deposits at the dermoepidermal junction in patients with vesiculobullous eruptions are diagnostic of dermatitis herpetiformis. Elevated titers of antinuclear antibodies, frequently with a peripheral pattern, and DNA antibodies as well as junctional deposits of immunoglobulins or complement or both in the apparently uninvolved skin are highly characteristic of systemic lupus erythematosus, more or less typical patterns of immunoglobulin and complement deposits in the lesion appear in discoid lupus erythematosus, porphyrias, and some other dermatoses."}
{"id": "PMID:1091216", "title": "Controlled double-blind comparative trial with fluprednylidene acetate cream and its base.", "content": "The effect of combined therapy with fluprednylidene acetate cream applied once daily plus cream base of the same formulation twice daily was tested in controlled double-blind contralateral investigations carried out on 31 in-patients with skin diseases which react to corticoids. Comparison with the 3 times daily application of the cream containing fluprednylidene and the 3 times daily application of a cream containing betamethasone 17-valerate did not show any differences in the result of treatment which was good to very good in each case within a 15-day trial period. No side effects were determined. The combined treatment with fluprednylidene acetate cream (once daily) and the cream base of the same formulation means that by economising on the use of the corticoid the risk involved in treatment is reduced and a therapeutic concept can be propounded which is suitable for all individual circumstances because the cream base used is kept constant.", "contents": "Controlled double-blind comparative trial with fluprednylidene acetate cream and its base. The effect of combined therapy with fluprednylidene acetate cream applied once daily plus cream base of the same formulation twice daily was tested in controlled double-blind contralateral investigations carried out on 31 in-patients with skin diseases which react to corticoids. Comparison with the 3 times daily application of the cream containing fluprednylidene and the 3 times daily application of a cream containing betamethasone 17-valerate did not show any differences in the result of treatment which was good to very good in each case within a 15-day trial period. No side effects were determined. The combined treatment with fluprednylidene acetate cream (once daily) and the cream base of the same formulation means that by economising on the use of the corticoid the risk involved in treatment is reduced and a therapeutic concept can be propounded which is suitable for all individual circumstances because the cream base used is kept constant."}
{"id": "PMID:1091217", "title": "Evidence for a neuromuscular disorder in methylmercury poisoning.", "content": "Data suggestive of a neuromuscular disorder responsive to neostigmine was uncovered in the course of electrophysiological testing of Iraqi patients poisoned by methylmercury. Subsequent neostigmine therapy produced a remarkable clinical improvement of the patients. Placebo substitution resulted in a substantial loss of testable strength that was restored when drug therapy was resumed.", "contents": "Evidence for a neuromuscular disorder in methylmercury poisoning. Data suggestive of a neuromuscular disorder responsive to neostigmine was uncovered in the course of electrophysiological testing of Iraqi patients poisoned by methylmercury. Subsequent neostigmine therapy produced a remarkable clinical improvement of the patients. Placebo substitution resulted in a substantial loss of testable strength that was restored when drug therapy was resumed."}
{"id": "PMID:1091218", "title": "Stability of normal human fecal flora during a chemically defined, low residue liquid diet.", "content": "Ten adult male volunteers (medical students) subsisted for seven days upon a chemically defined, low residue liquid diet, and consumed 1200-1800 calories per day. All stools were collected; three were cultured within the hour-a prediet stool, one collected on the seventh day, and a postdiet stool. Specimens were diluted anaerobically, and anaerobic cultures were streaked upon plates of prereduced agar media and incubated in Brewer jars. During the low residue diet, total fecal mass was relatively small and each subject passed only two or three stools. The mean reduction in daily fecal output was 70%. Mean counts of total aerobes were 10-7/gm throughout the study, and mean counts of total anaerobes were 10-10/gm. There was no overgrowth by opportunistic bacteria or fungi. The low residue food did not alter fecal flora; there was neither disappearance nor reduction of any bacterial group.", "contents": "Stability of normal human fecal flora during a chemically defined, low residue liquid diet. Ten adult male volunteers (medical students) subsisted for seven days upon a chemically defined, low residue liquid diet, and consumed 1200-1800 calories per day. All stools were collected; three were cultured within the hour-a prediet stool, one collected on the seventh day, and a postdiet stool. Specimens were diluted anaerobically, and anaerobic cultures were streaked upon plates of prereduced agar media and incubated in Brewer jars. During the low residue diet, total fecal mass was relatively small and each subject passed only two or three stools. The mean reduction in daily fecal output was 70%. Mean counts of total aerobes were 10-7/gm throughout the study, and mean counts of total anaerobes were 10-10/gm. There was no overgrowth by opportunistic bacteria or fungi. The low residue food did not alter fecal flora; there was neither disappearance nor reduction of any bacterial group."}
{"id": "PMID:1091219", "title": "Pyeloureterostomy reconstruction in human renal transplantation.", "content": "Pyelouretersotomy or ureteroureterostomy was the urinary tract reconstructive procedure in 114 of 132 consecutive renal transplants performed at the Massachusetts General Hospital from September 1967 to December 1973. Anastomotic leakage was the only complication related to failure of pyeloureterostomy or ureteroureterostomy. This occurred in nine transplants (7.9%). Since 1970, only four anastomotic leaks have occurred in 79 consecutive pyeloureterostomies or ureteroureterostomies. Six of the nine primary anastomotic leaks were corrected either by retrograde ureteral catheter drainage or re-suturing of the anastomotic defect. Only one patient died as a result of complications following a leak. The operative technique is described with emphasis on the important details believed necessary for consistent success in performing this procedure.", "contents": "Pyeloureterostomy reconstruction in human renal transplantation. Pyelouretersotomy or ureteroureterostomy was the urinary tract reconstructive procedure in 114 of 132 consecutive renal transplants performed at the Massachusetts General Hospital from September 1967 to December 1973. Anastomotic leakage was the only complication related to failure of pyeloureterostomy or ureteroureterostomy. This occurred in nine transplants (7.9%). Since 1970, only four anastomotic leaks have occurred in 79 consecutive pyeloureterostomies or ureteroureterostomies. Six of the nine primary anastomotic leaks were corrected either by retrograde ureteral catheter drainage or re-suturing of the anastomotic defect. Only one patient died as a result of complications following a leak. The operative technique is described with emphasis on the important details believed necessary for consistent success in performing this procedure."}
{"id": "PMID:1091220", "title": "Pneumonectomy by \"en masse\" stapling of hilar vessels.", "content": "Since the development of automatic surgical stapling instruments in the Soviet Union in the 1950s and their description in the American literature in 1961, American surgeons have used them for a variety of pulmonary resections. However, there is no account of their use in this country for pneumonectomy by \"en masse\" stapling of the hilar structures. We have used this whenever it was applicable in 41 consecutive pneumonectomies since 1967. There were 2 postoperative deaths, 1 due to pulmonary embolism and the other to myocardial infarction. There were no complications attributable to the operative technique used. The only empyema developed nine months postoperatively and was not accompanied by bronchial fistula. Cancer-free survival was 43% at two and one-half years and 33% at five years. The technique of en masse stapling of hilar vessels renders pneumonectomy easier, safer, and faster than the technique of individual ligation.", "contents": "Pneumonectomy by \"en masse\" stapling of hilar vessels. Since the development of automatic surgical stapling instruments in the Soviet Union in the 1950s and their description in the American literature in 1961, American surgeons have used them for a variety of pulmonary resections. However, there is no account of their use in this country for pneumonectomy by \"en masse\" stapling of the hilar structures. We have used this whenever it was applicable in 41 consecutive pneumonectomies since 1967. There were 2 postoperative deaths, 1 due to pulmonary embolism and the other to myocardial infarction. There were no complications attributable to the operative technique used. The only empyema developed nine months postoperatively and was not accompanied by bronchial fistula. Cancer-free survival was 43% at two and one-half years and 33% at five years. The technique of en masse stapling of hilar vessels renders pneumonectomy easier, safer, and faster than the technique of individual ligation."}
{"id": "PMID:1091224", "title": "The spectrum concept of schizophrenia. Problems for diagnostic practice.", "content": "The spectrum concept of schizophrenia posits that a number of psychopathologic states, both psychotic and nonpsychotic, may share some genetic basis with schizophrenia, and may therefore constitute, together with schizophrenia itself, a genetic spectrum of schizophrenic disorders. While this is a valuable and promising research concept, its application to diagnostic practice could broaden the boundaries of schizophrenia to include patients with other conditions, thus rendering a larger population at risk for the untoward effects of the schizophrenia disgnosis itself. This may have already occurred in the Soviet Union, where a diagnostic system derived from a variant of the spectrum concept is used in routine diagnostic practice. The adoption of a spectrum-based diagnostic system should await further research on the boundaries of the spectrum, and more detailed descriptions of confirmed spectrum states.", "contents": "The spectrum concept of schizophrenia. Problems for diagnostic practice. The spectrum concept of schizophrenia posits that a number of psychopathologic states, both psychotic and nonpsychotic, may share some genetic basis with schizophrenia, and may therefore constitute, together with schizophrenia itself, a genetic spectrum of schizophrenic disorders. While this is a valuable and promising research concept, its application to diagnostic practice could broaden the boundaries of schizophrenia to include patients with other conditions, thus rendering a larger population at risk for the untoward effects of the schizophrenia disgnosis itself. This may have already occurred in the Soviet Union, where a diagnostic system derived from a variant of the spectrum concept is used in routine diagnostic practice. The adoption of a spectrum-based diagnostic system should await further research on the boundaries of the spectrum, and more detailed descriptions of confirmed spectrum states."}
{"id": "PMID:1091236", "title": "Sex research and social change.", "content": "The history of scientific sex research which emerged at the turn of the century with the exemplary work of Sigmund Freud and Havelock Ellis has been complexly interactive with changing general social conditions, specific trends in sexual conduct, the content of sexual ideologies, and the developing techniques of scientific inquiry. The earliest sex researchers, although serving to bring sexuality out of the Victorian cold and into the center of human development based their views of sexuality on control-repression and drive models. The Freudian tradition was especially influential in general intellectual matters and was probably the most important in the development of twentieth-century sexual ideologies. Beginning in the 1920s and culminating in the work of Kinsey in the 1940s and 1950s, a tradiition of social bookkeeping began focusing on the sexual behavior of relatively normal persons. Methodologically such studies moved away from the case history and from populations who were defined as criminal or neurotic. At the same time, general social changes were occurring that were directly affecting the rates and directions of sexual conduct in the society. The work of Alfred Kinsey charted these changes and in turn influenced public attitudes, public policy, and research interests during the 1950s and 1960s. The work of other researchers began to fill in the picture of sexual conduct in the society from a survey point of view, and some workers began studies in sexual deviance that focused anew on homosexuality and prostitution. The work of William Masters and Virginia Johnson served to open the door to studies of sexual anatomy and physiology by applying well-known techniques to the laboratory study of the sexual. While the biological tradition is still strong in the discussion of the sexual, new emphases are being placed on a cognitive-social learning persepctive that emphasizes the nonbiological factors in sexual development. Major changes have occurred in the sexual backdrop of the society in the 1960s, and while changes in sexual conduct have been less than revolutionary, they have occurred in a number of areas (contraception, abortion) that have directly influenced societal practices. Sex research and the sex researcher have played an important role in providing benchmarks for sexual practices, illuminating general understanding, and providing the content for ideiolicaal debates about the right and wrong of sexuality in the society. In few areas of research have researchers had such an important role in the debate over the measning and significance of the behavior they have studied.", "contents": "Sex research and social change. The history of scientific sex research which emerged at the turn of the century with the exemplary work of Sigmund Freud and Havelock Ellis has been complexly interactive with changing general social conditions, specific trends in sexual conduct, the content of sexual ideologies, and the developing techniques of scientific inquiry. The earliest sex researchers, although serving to bring sexuality out of the Victorian cold and into the center of human development based their views of sexuality on control-repression and drive models. The Freudian tradition was especially influential in general intellectual matters and was probably the most important in the development of twentieth-century sexual ideologies. Beginning in the 1920s and culminating in the work of Kinsey in the 1940s and 1950s, a tradiition of social bookkeeping began focusing on the sexual behavior of relatively normal persons. Methodologically such studies moved away from the case history and from populations who were defined as criminal or neurotic. At the same time, general social changes were occurring that were directly affecting the rates and directions of sexual conduct in the society. The work of Alfred Kinsey charted these changes and in turn influenced public attitudes, public policy, and research interests during the 1950s and 1960s. The work of other researchers began to fill in the picture of sexual conduct in the society from a survey point of view, and some workers began studies in sexual deviance that focused anew on homosexuality and prostitution. The work of William Masters and Virginia Johnson served to open the door to studies of sexual anatomy and physiology by applying well-known techniques to the laboratory study of the sexual. While the biological tradition is still strong in the discussion of the sexual, new emphases are being placed on a cognitive-social learning persepctive that emphasizes the nonbiological factors in sexual development. Major changes have occurred in the sexual backdrop of the society in the 1960s, and while changes in sexual conduct have been less than revolutionary, they have occurred in a number of areas (contraception, abortion) that have directly influenced societal practices. Sex research and the sex researcher have played an important role in providing benchmarks for sexual practices, illuminating general understanding, and providing the content for ideiolicaal debates about the right and wrong of sexuality in the society. In few areas of research have researchers had such an important role in the debate over the measning and significance of the behavior they have studied."}
{"id": "PMID:1091237", "title": "Molecular pathogenesis of systemic lupus erythematosus.", "content": "The study of patients with systemic lupus erythematosus revealed the close association of the disease with measles--or a related virus. High titres of antibodies to measles virus were found in patients that correlated with the course of the disease. Immunofluorescence tests revealed measles virus or a related antigen in lupus-affected tissues. Inclusion bodies consisting of paramyxovirus-like ribonucleoprotein structures were regularly detected in both affected tissues and leukocytes. Molecular hybridization of measles virus RNA with DNA from the affected tissues showed that DNA transcripts of measles or a closely related virus are integrated in the cellular nuclear DNA. Possible pathogenetic mechanisms of the disease are discussed.", "contents": "Molecular pathogenesis of systemic lupus erythematosus. The study of patients with systemic lupus erythematosus revealed the close association of the disease with measles--or a related virus. High titres of antibodies to measles virus were found in patients that correlated with the course of the disease. Immunofluorescence tests revealed measles virus or a related antigen in lupus-affected tissues. Inclusion bodies consisting of paramyxovirus-like ribonucleoprotein structures were regularly detected in both affected tissues and leukocytes. Molecular hybridization of measles virus RNA with DNA from the affected tissues showed that DNA transcripts of measles or a closely related virus are integrated in the cellular nuclear DNA. Possible pathogenetic mechanisms of the disease are discussed."}
{"id": "PMID:1091238", "title": "Early events in myxovirus replication: immunofluorescent spots.", "content": "Indirect immunofluorescent staining of Ehrlich ascites tumor cells infected with two influenza A strains, WSA (HON1) and TUR (Hav1 Nav3), revealed early fluorescent spots which became detectable in the cytoplasm within 30 minutes of infection, before the nucleoprotein antigen appeared in the nucleus. These spots seemed to be linked to some structural antigen of the virus not identical with hemagglutinin, neuraminidase, or nucleoprotein. Their formation was not inhibited by actinomycin, p-fluorophenylalanine or amantadine in concentrations sufficient to block viral replication; amantadine led to an altered time course of spot evolution and to the emergence of coarser spots. The exact serologic specificity of early spots remains to be worked out but appears to differ from that of known influenza A antigens.", "contents": "Early events in myxovirus replication: immunofluorescent spots. Indirect immunofluorescent staining of Ehrlich ascites tumor cells infected with two influenza A strains, WSA (HON1) and TUR (Hav1 Nav3), revealed early fluorescent spots which became detectable in the cytoplasm within 30 minutes of infection, before the nucleoprotein antigen appeared in the nucleus. These spots seemed to be linked to some structural antigen of the virus not identical with hemagglutinin, neuraminidase, or nucleoprotein. Their formation was not inhibited by actinomycin, p-fluorophenylalanine or amantadine in concentrations sufficient to block viral replication; amantadine led to an altered time course of spot evolution and to the emergence of coarser spots. The exact serologic specificity of early spots remains to be worked out but appears to differ from that of known influenza A antigens."}
{"id": "PMID:1091243", "title": "The lens.", "content": "The subjects to be reviewed are as follows: (1) Anatomy, histology, and pathology. (2) Genetic and congenital anomalies. (3) Cataract. (Under cataract we shall consider the following: incidence and prevention of blindness from cataract; formation of an experimental production of cataract; cataract related to ocular and systemic disease; cataract related to medication, trauma, and radiation; medical treatment of cataract; cataract surgery; complications of cataract surgery; optical correction of aphakia.) (4) Exfoliation syndrome.", "contents": "The lens. The subjects to be reviewed are as follows: (1) Anatomy, histology, and pathology. (2) Genetic and congenital anomalies. (3) Cataract. (Under cataract we shall consider the following: incidence and prevention of blindness from cataract; formation of an experimental production of cataract; cataract related to ocular and systemic disease; cataract related to medication, trauma, and radiation; medical treatment of cataract; cataract surgery; complications of cataract surgery; optical correction of aphakia.) (4) Exfoliation syndrome."}
{"id": "PMID:1091245", "title": "Current management of non-Hodgkin malignant lymphoma of the head and neck.", "content": "Older nomenclature for non-Hodgkin malignant lymphoma has undergone revision to more accurate terminology based on cytology and histological pattern. Presentation of non-Hodgkin malignant lymphoma above the supraclavicular fossa demands through investigation to rule out occult disease in bone marrow and the intra-abdominal space. This is accomplished by appropriate clinical evaluation including lymphangiogram and exploratory laparotomy. However, the value of exploratory laparotomy for staging on increased survival rates in non-Hodgkin malignant lymphoma of the head and neck has not been established.", "contents": "Current management of non-Hodgkin malignant lymphoma of the head and neck. Older nomenclature for non-Hodgkin malignant lymphoma has undergone revision to more accurate terminology based on cytology and histological pattern. Presentation of non-Hodgkin malignant lymphoma above the supraclavicular fossa demands through investigation to rule out occult disease in bone marrow and the intra-abdominal space. This is accomplished by appropriate clinical evaluation including lymphangiogram and exploratory laparotomy. However, the value of exploratory laparotomy for staging on increased survival rates in non-Hodgkin malignant lymphoma of the head and neck has not been established."}
{"id": "PMID:1091246", "title": "Transsternal radical neck dissection. Postoperative complications and management.", "content": "In 1962, Sisson et al reported the use of the transsternal radical neck dissection for carcinoma recurring in the peristomal area after laryngectomy. We have performed over 50 transsternal radical neck dissections in the past 14 years. A significant number of early cases succumbed to the postoperative problems of fistula, infection, and large vessel rupture. Our two cases illustrate intraoperative and postoperative complications. Management of these complications is discussed. Morbidity and mortality has decreased as we have gained experience in the management of these problems.", "contents": "Transsternal radical neck dissection. Postoperative complications and management. In 1962, Sisson et al reported the use of the transsternal radical neck dissection for carcinoma recurring in the peristomal area after laryngectomy. We have performed over 50 transsternal radical neck dissections in the past 14 years. A significant number of early cases succumbed to the postoperative problems of fistula, infection, and large vessel rupture. Our two cases illustrate intraoperative and postoperative complications. Management of these complications is discussed. Morbidity and mortality has decreased as we have gained experience in the management of these problems."}
{"id": "PMID:1091247", "title": "Repair of extended laryngotracheal stenosis.", "content": "The most challenging laryngotracheal stenosis are those that exceed 5 cm in length and involve more than one area of the larynx and trachea contiguously. A successful technique for the repair of these injuries with a three-stage laryngotracheal trough was created followed by anterior tracheal wall replacement with a skin-Marlex-muscle pedicle flap.", "contents": "Repair of extended laryngotracheal stenosis. The most challenging laryngotracheal stenosis are those that exceed 5 cm in length and involve more than one area of the larynx and trachea contiguously. A successful technique for the repair of these injuries with a three-stage laryngotracheal trough was created followed by anterior tracheal wall replacement with a skin-Marlex-muscle pedicle flap."}
{"id": "PMID:1091248", "title": "Surgical repair of facial nerve branches. An analysis of different sheathing and suturing techniques.", "content": "The distal branches of the facial nerve are sometimes severed in various forms of trauma. These distal branches respond to repair differently than do the main trunk or other peripheral nerves. Actual axon counts must be done to determine the percentage of regrowth rather than measuring nerve diameter. Physiologic and histologic techniques were used to analyze sheathing and suturing techniques in the repair of facial nerve branches in rabbits. Repair sites were examined at various intervals up to four months after repair. Very small suture material (10-0) must be used. Vein sheaths caused decreased axon regrowth, and strictures occurred when silicone elastomer sheaths were used. Seven stitches provided the best results in terms of axon regrowth, prevention of neuromas, and return of function. Three sutures or a continuous stitch produced poorer overall results.", "contents": "Surgical repair of facial nerve branches. An analysis of different sheathing and suturing techniques. The distal branches of the facial nerve are sometimes severed in various forms of trauma. These distal branches respond to repair differently than do the main trunk or other peripheral nerves. Actual axon counts must be done to determine the percentage of regrowth rather than measuring nerve diameter. Physiologic and histologic techniques were used to analyze sheathing and suturing techniques in the repair of facial nerve branches in rabbits. Repair sites were examined at various intervals up to four months after repair. Very small suture material (10-0) must be used. Vein sheaths caused decreased axon regrowth, and strictures occurred when silicone elastomer sheaths were used. Seven stitches provided the best results in terms of axon regrowth, prevention of neuromas, and return of function. Three sutures or a continuous stitch produced poorer overall results."}
{"id": "PMID:1091249", "title": "Modified nondelayed forehead flap.", "content": "The versatility and benefit of the forehead flap need not be lost when the external carotid artery system has been ligated. By basing the forehead flap laterally on the scalp, nourishment from the opposite external carotid artery gives the head and neck surgeon another advantage in reconstruction with nondelayed expedience. We report three cases of wide cheek ablative surgery, external carotid ligation, and utilization of the forehead flap. The occasions to use this modified nondelayed forehead flap are few, but it offers the same advantage without staging. It definitely can be used in any situation requiring a forehead flap when the external carotid artery system has been ligated.", "contents": "Modified nondelayed forehead flap. The versatility and benefit of the forehead flap need not be lost when the external carotid artery system has been ligated. By basing the forehead flap laterally on the scalp, nourishment from the opposite external carotid artery gives the head and neck surgeon another advantage in reconstruction with nondelayed expedience. We report three cases of wide cheek ablative surgery, external carotid ligation, and utilization of the forehead flap. The occasions to use this modified nondelayed forehead flap are few, but it offers the same advantage without staging. It definitely can be used in any situation requiring a forehead flap when the external carotid artery system has been ligated."}
{"id": "PMID:1091250", "title": "Incus window in transposition.", "content": "In aiding the positioning of the incus in incus transposition, a hole, the incus window, is drilled completely through the body of the incus. Through the window the surgeon has a visual as well as a tactile reference to the head of the stapes. At one year results show that 182 of 201 patients closed the air-bone gap to within 20 dB of the preoperative bone level.", "contents": "Incus window in transposition. In aiding the positioning of the incus in incus transposition, a hole, the incus window, is drilled completely through the body of the incus. Through the window the surgeon has a visual as well as a tactile reference to the head of the stapes. At one year results show that 182 of 201 patients closed the air-bone gap to within 20 dB of the preoperative bone level."}
{"id": "PMID:1091252", "title": "A quantitative study of the effect of endotoxin on embryonating chicken eggs.", "content": "A quantitative study of the effect of endotoxin on embryonating chicken eggs revealed the importance of several variables. In the study, the chorioallanotic membrane was detached (i.e.,dropped) from the eggshell membrane on the 9th and 11th incubation days. Various doses of endotoxin in various amounts of diluent were applied on the dropped membrane on the 11th day. Embryo deaths were greater when the membrane was dropped on the 11th day. Within the limits of our trial, the variables were, in decreasing order of significance: 1) the day the membrane was dropped; 2) the amount of endotoxin applied; and 3) an interaction between 1 and 2. The volume of diluent had no significant effect on mortality. The importance of these variables is discussed with reference to the design of additional experiments with endotoxin.", "contents": "A quantitative study of the effect of endotoxin on embryonating chicken eggs. A quantitative study of the effect of endotoxin on embryonating chicken eggs revealed the importance of several variables. In the study, the chorioallanotic membrane was detached (i.e.,dropped) from the eggshell membrane on the 9th and 11th incubation days. Various doses of endotoxin in various amounts of diluent were applied on the dropped membrane on the 11th day. Embryo deaths were greater when the membrane was dropped on the 11th day. Within the limits of our trial, the variables were, in decreasing order of significance: 1) the day the membrane was dropped; 2) the amount of endotoxin applied; and 3) an interaction between 1 and 2. The volume of diluent had no significant effect on mortality. The importance of these variables is discussed with reference to the design of additional experiments with endotoxin."}
{"id": "PMID:1091253", "title": "An outbreak of type-C botulism in three-weeek-old broilers.", "content": "Botulism was diagnosed in 3-week-old broilers from clinical signs, absence of postmortem and histopathological lesions, and demonstration of toxin in the serum of comatose birds. Passive immunization of mice with Clostridium botulinum type-C antitoxin protected against a challenge with serum from comatose birds containing the Cl. botulinum toxin. Total mortality for the grow-out period exceeded 27% and was not altered by water medication with penicillin. Bacitracin at 100 g per ton reduced mortality to 5-7 birds per day.", "contents": "An outbreak of type-C botulism in three-weeek-old broilers. Botulism was diagnosed in 3-week-old broilers from clinical signs, absence of postmortem and histopathological lesions, and demonstration of toxin in the serum of comatose birds. Passive immunization of mice with Clostridium botulinum type-C antitoxin protected against a challenge with serum from comatose birds containing the Cl. botulinum toxin. Total mortality for the grow-out period exceeded 27% and was not altered by water medication with penicillin. Bacitracin at 100 g per ton reduced mortality to 5-7 birds per day."}
{"id": "PMID:1091254", "title": "Plastic embedding of avian tissues for diagnostic histopathology.", "content": "A series of photomicrographs show the histologic features of avian tissues embedded in plastic and sectioned on glass knives. The techniques described gave superior resolution, less cell shrinkage, and fewer sectioning artifacts than sections prepared with conventional paraffin methods.", "contents": "Plastic embedding of avian tissues for diagnostic histopathology. A series of photomicrographs show the histologic features of avian tissues embedded in plastic and sectioned on glass knives. The techniques described gave superior resolution, less cell shrinkage, and fewer sectioning artifacts than sections prepared with conventional paraffin methods."}
{"id": "PMID:1091255", "title": "Direct immunofluorescent technique in diagnosis of experimental salmonellosis of turkeys.", "content": "Young turkey poults were experimentally infected with Salmonella heidelberg to evaluate the standard tube agglutination test (TAT) and direct fluorescent antibody test (DFAT), and to correlate results of these procedures with the spread pattern of the organisms in the feces using conventional culture procedures for the detection and isolation of S heidelberg from cloacal swabs. The DFAT test detected fecal excretors considerably longer than bacterial isolation and gave better results. The cultural method was not the most reliable and sensitive method, especially when compared with the DFAT. All control birds remained negative bacteriologically serologically, and with the DFAT during the observation period.", "contents": "Direct immunofluorescent technique in diagnosis of experimental salmonellosis of turkeys. Young turkey poults were experimentally infected with Salmonella heidelberg to evaluate the standard tube agglutination test (TAT) and direct fluorescent antibody test (DFAT), and to correlate results of these procedures with the spread pattern of the organisms in the feces using conventional culture procedures for the detection and isolation of S heidelberg from cloacal swabs. The DFAT test detected fecal excretors considerably longer than bacterial isolation and gave better results. The cultural method was not the most reliable and sensitive method, especially when compared with the DFAT. All control birds remained negative bacteriologically serologically, and with the DFAT during the observation period."}
{"id": "PMID:1091270", "title": "[Pivampicillin and ampicillin in experimental E. coli pyelonephritis].", "content": "The antimicrobiol activity of 6-[D(--)-alpha-aminophenylacetamido]-penicillanic acid-(pivaloyloxymethyl)-ester (privampicillin) was tested in comparison to ampicillin during the acute ascending pyelonephritis of the rat caused by E. coli. Two groups of rats were treated with different drug concentrations; group I:3 times 70 mg/kg/day pivampicillin and 3 times 50 mg/kg/day ampicillin, group II: 3 times 70 mg/kg/day pivampicillin and 3 times 150 mg/kg/day ampicillin; controls were inoculated three times daily with 1.0 ml physiological saline. A higher activity of pivampicillin was found in the first group as proved by bacterial counting in the left and the right renal parenchyma as well as in the urine. In the second group the efficacy of ampicillin was increased.", "contents": "[Pivampicillin and ampicillin in experimental E. coli pyelonephritis]. The antimicrobiol activity of 6-[D(--)-alpha-aminophenylacetamido]-penicillanic acid-(pivaloyloxymethyl)-ester (privampicillin) was tested in comparison to ampicillin during the acute ascending pyelonephritis of the rat caused by E. coli. Two groups of rats were treated with different drug concentrations; group I:3 times 70 mg/kg/day pivampicillin and 3 times 50 mg/kg/day ampicillin, group II: 3 times 70 mg/kg/day pivampicillin and 3 times 150 mg/kg/day ampicillin; controls were inoculated three times daily with 1.0 ml physiological saline. A higher activity of pivampicillin was found in the first group as proved by bacterial counting in the left and the right renal parenchyma as well as in the urine. In the second group the efficacy of ampicillin was increased."}
{"id": "PMID:1091271", "title": "[Cellular kinetics of the primary immune response after application of phytohemagglutinin].", "content": "The Jerne technique, a proved method for detecting the number of plaque-forming cells (PFC) in spleen, shows an immunosuppressive effect of phytohemagglutine (PHA) on primary immune response. Kinetic studies are done on 300 NMRI mice, considering the number of PFC/10-8 spleen cells. Significant suppressions of the primary immune response are found if PHA is applied either one day before or together with antigen provided that studies are done 3, 4 or 5 days after immunization with sheep red blood cells (SRBC). The role of activated T-lymphocytes concerning the diminution of immune response is discussed.", "contents": "[Cellular kinetics of the primary immune response after application of phytohemagglutinin]. The Jerne technique, a proved method for detecting the number of plaque-forming cells (PFC) in spleen, shows an immunosuppressive effect of phytohemagglutine (PHA) on primary immune response. Kinetic studies are done on 300 NMRI mice, considering the number of PFC/10-8 spleen cells. Significant suppressions of the primary immune response are found if PHA is applied either one day before or together with antigen provided that studies are done 3, 4 or 5 days after immunization with sheep red blood cells (SRBC). The role of activated T-lymphocytes concerning the diminution of immune response is discussed."}
{"id": "PMID:1091272", "title": "[Clinical study on five depot neuroleptics.  Comparison of effective profiles of fluphenazinedecanoate, fluspirilene, penfluridol, perphenazine-enanthate and pipothiazinepalmitate].", "content": "Five depot neuroleptics (fluphenazinedeconoate, fluspirilene, pipothiazinepalmitate, penfluridol and perphenazine-enanthate) were compared based on clinical trials in subacute and chronic schizophrenic patients.  The psychopathological symptoms were documented by means of the AMP-system.  Statistical analyses showed several differences between the effects of the five substances.  The AMP-system proved a useful instrument to differentiate similar drugs.", "contents": "[Clinical study on five depot neuroleptics.  Comparison of effective profiles of fluphenazinedecanoate, fluspirilene, penfluridol, perphenazine-enanthate and pipothiazinepalmitate]. Five depot neuroleptics (fluphenazinedeconoate, fluspirilene, pipothiazinepalmitate, penfluridol and perphenazine-enanthate) were compared based on clinical trials in subacute and chronic schizophrenic patients.  The psychopathological symptoms were documented by means of the AMP-system.  Statistical analyses showed several differences between the effects of the five substances.  The AMP-system proved a useful instrument to differentiate similar drugs."}
{"id": "PMID:1091282", "title": "Intermediate coronary care. A controlled trial.", "content": "A controlled trial of intermediate coronary care was carried out over a five-year period at a district general hospital. One thousand male patients under 65 were allocated at random into a group kept in the same ward as the coronary care unit (CCU) and a control group discharged from the CCU to a general medical ward. The intermediate care patients were nursed by the CCU staff, resuscitation equipment was immediately available and there was an efficient emergency call system. The mortality was the same in both groups and no more patients survived cardiac arrest to leave hospital in the intermediate care group than among the controls, though initial resuscitation was more often successful. The failure of intermediate coronary care was attributed to the rarity of primary ventricular fibrillation after discharge from the CCU.", "contents": "Intermediate coronary care. A controlled trial. A controlled trial of intermediate coronary care was carried out over a five-year period at a district general hospital. One thousand male patients under 65 were allocated at random into a group kept in the same ward as the coronary care unit (CCU) and a control group discharged from the CCU to a general medical ward. The intermediate care patients were nursed by the CCU staff, resuscitation equipment was immediately available and there was an efficient emergency call system. The mortality was the same in both groups and no more patients survived cardiac arrest to leave hospital in the intermediate care group than among the controls, though initial resuscitation was more often successful. The failure of intermediate coronary care was attributed to the rarity of primary ventricular fibrillation after discharge from the CCU."}
{"id": "PMID:1091283", "title": "Maximal intestinal absorption of digoxin, and its relation to steady state plasma concentration.", "content": "In a group of 8 volunteers, peak plasma digoxin concentrations and areas under 80-hour plasma concentration curves were significantly greater after 1 mg digoxin in paediatric elixir than after 4 0,25 mg tablets. Mean cumulative urinary excretion of digoxin over 12 days was 46.4 per cent after tablets, 53.6 per cent after elixir, and 70.8 per cent after intravenous injection. Mean percentage absorption was estimated to be 63 per cent from tablets and 75 per cent from elixir, but considerable between-subject variation was noted. Individual estimates of percentage absorption were significantly correlated with plasma concentrations in the steady state. Computer programmes to relate steady state plasma concentration to oral digoxin dosage take no account of absorptive capacity, are limited to gross approximations, and cannot replace determination of plasma concentration to assess the degree of digitalization.", "contents": "Maximal intestinal absorption of digoxin, and its relation to steady state plasma concentration. In a group of 8 volunteers, peak plasma digoxin concentrations and areas under 80-hour plasma concentration curves were significantly greater after 1 mg digoxin in paediatric elixir than after 4 0,25 mg tablets. Mean cumulative urinary excretion of digoxin over 12 days was 46.4 per cent after tablets, 53.6 per cent after elixir, and 70.8 per cent after intravenous injection. Mean percentage absorption was estimated to be 63 per cent from tablets and 75 per cent from elixir, but considerable between-subject variation was noted. Individual estimates of percentage absorption were significantly correlated with plasma concentrations in the steady state. Computer programmes to relate steady state plasma concentration to oral digoxin dosage take no account of absorptive capacity, are limited to gross approximations, and cannot replace determination of plasma concentration to assess the degree of digitalization."}
{"id": "PMID:1091284", "title": "Fluorescence energy transfer between ligand binding sites on aspartate transcarbamylase.", "content": "The method of fluorescence energy transfer is used to measure the distances between several sites on aspartate transcarbamylase. Both fluorescence steady-state and lifetime techniques are used. When the tryptophans on the catalytic subunit are the fluorescent donor groups, either pyridoxamine phosphate, covalently bound to an amino group at the active site, or 8-anilino-1-naphthalenesulfonate, noncovalently bound at the active site, is the acceptor group. The distance between tryptophan and the active site is calculated to be 2e A assuming that the fluorescence of only one tryptophan per catalytic polypeptide chain is quenched by the acceptor or 27 A assuming that both tryptophans on a catalytic chain are equally quenched. The pyridoxamine phosphate label is also used as the fluorescent donor with mercurinitrophenol bound to the sulfhydryl group of the catalytic subunit as the energy acceptor. For this pair of labels the active site is determined to be very close to the sulfhydryl group on the same catalytic chain and 26 A from the sulfhydryl groups on the other chains of the catalytic trimer. In experiments with pyridoxamine phosphate at the active site as the donor and 8-anilino-1-naphthalenesulfonate at the active site as the acceptor, a distance of 26 A between active sites of a catalytic trimer is found. No energy transfer is observed from pyridoxamine phosphate at the active site to a fluorescamine derivative of cytidine 5'-triphosphate at the regulatory site. This implies that these groups are separated by at least 42 A in the native enzyme. All of the distances are calculated using the assumption of rapid rotation of donor and acceptor dipole moments relative to the donor fluorescence lifetime. Fluorescence polarization measurements suggest this assumption does not produce a significant error in the calculated distances. The distances between the various sites are related to the subunit structure of aspartate transcarbamylase.", "contents": "Fluorescence energy transfer between ligand binding sites on aspartate transcarbamylase. The method of fluorescence energy transfer is used to measure the distances between several sites on aspartate transcarbamylase. Both fluorescence steady-state and lifetime techniques are used. When the tryptophans on the catalytic subunit are the fluorescent donor groups, either pyridoxamine phosphate, covalently bound to an amino group at the active site, or 8-anilino-1-naphthalenesulfonate, noncovalently bound at the active site, is the acceptor group. The distance between tryptophan and the active site is calculated to be 2e A assuming that the fluorescence of only one tryptophan per catalytic polypeptide chain is quenched by the acceptor or 27 A assuming that both tryptophans on a catalytic chain are equally quenched. The pyridoxamine phosphate label is also used as the fluorescent donor with mercurinitrophenol bound to the sulfhydryl group of the catalytic subunit as the energy acceptor. For this pair of labels the active site is determined to be very close to the sulfhydryl group on the same catalytic chain and 26 A from the sulfhydryl groups on the other chains of the catalytic trimer. In experiments with pyridoxamine phosphate at the active site as the donor and 8-anilino-1-naphthalenesulfonate at the active site as the acceptor, a distance of 26 A between active sites of a catalytic trimer is found. No energy transfer is observed from pyridoxamine phosphate at the active site to a fluorescamine derivative of cytidine 5'-triphosphate at the regulatory site. This implies that these groups are separated by at least 42 A in the native enzyme. All of the distances are calculated using the assumption of rapid rotation of donor and acceptor dipole moments relative to the donor fluorescence lifetime. Fluorescence polarization measurements suggest this assumption does not produce a significant error in the calculated distances. The distances between the various sites are related to the subunit structure of aspartate transcarbamylase."}
{"id": "PMID:1091285", "title": "The quaternary structure of citrate synthase from Escherichia coli K12.", "content": "A combination of equilibrium ultracentrifugation and polyacrylamide gel electrophoresis techniques has been used to establish the quaternary structure of citrate synthase from acetate-grown Escherichia coli K12 3000. In polyacrylamide gels containing 0.1% sodium dodecyl sulfate (SDS), the pure enzyme showed one major band whose mobility was consistent with a molecular weight of 46,000 plus or minus 2000 g/mol, and a little material of 87,000 plus or minus 5000 g/mol. When first cross-linked with dimethyl suberimidate and then submitted to electrophoresis in SDS, citrate synthase showed six bands, in widely different amounts, whose apparent molecular weights were almost integral multiples of 47,000 g/mol. The dimer was the major product of the cross-linking procedure. In 6 M guanidine HCl at pH 7.0, citrate synthase behaved as a single component in high-speed sedimentation equilibrium experiments, with a weight average molecular weight of 43,400 plus or minus 300 g/mol. The molecular weight of native citrate synthase was investigated by high-speed sedimentation equilibrium ultracentrifugation under different conditions of pH and KCl concentration. In 0.02 M Tris-Cl at pH 7.0 and 7.8, the enzyme was a mixture of oligomers, with species ranging from monomer (47,000 g/mol) to greater than decamer being present. At pH 9.0, only dimer was seen (94,000 g/mol). Large aggregates were present at pH 10.0. The addition of small amounts of KCl, a potent activator of the enzyme, simplified the mixture of oligomers considerably at pH 7.8. A detailed analysis of the data with 0.05 M KCl indicated that dimer and hexamer were the only species present, with marked nonideality. Increasing the KCl concentration to 0.10 M converted all the enzyme to hexamer. The amino acid composition of E. coli citrate synthase was presented. Taken together with peptide mapping experiments of others (J. A. Wright and B. D. Sanwal (1971), J. Biol. Chem. 246 1689), it indicates that the subunits have all the same or very similar amino acid sequences. The dansylation method revealed only methionine at the N-termini of the citrate synthase polypeptide chains. Citrate synthase from E. coli thus resembles the enzyme from eukaryotes in that it consists of subunits weighing just under 50,000 g/mol, although these subunits are more highly aggregated in the bacterial enzyme under most conditions. This conclusion is in disagreement with that of Wright and Sanwal (1971, see above), who reported a subunit size of 62,000 g/mol.", "contents": "The quaternary structure of citrate synthase from Escherichia coli K12. A combination of equilibrium ultracentrifugation and polyacrylamide gel electrophoresis techniques has been used to establish the quaternary structure of citrate synthase from acetate-grown Escherichia coli K12 3000. In polyacrylamide gels containing 0.1% sodium dodecyl sulfate (SDS), the pure enzyme showed one major band whose mobility was consistent with a molecular weight of 46,000 plus or minus 2000 g/mol, and a little material of 87,000 plus or minus 5000 g/mol. When first cross-linked with dimethyl suberimidate and then submitted to electrophoresis in SDS, citrate synthase showed six bands, in widely different amounts, whose apparent molecular weights were almost integral multiples of 47,000 g/mol. The dimer was the major product of the cross-linking procedure. In 6 M guanidine HCl at pH 7.0, citrate synthase behaved as a single component in high-speed sedimentation equilibrium experiments, with a weight average molecular weight of 43,400 plus or minus 300 g/mol. The molecular weight of native citrate synthase was investigated by high-speed sedimentation equilibrium ultracentrifugation under different conditions of pH and KCl concentration. In 0.02 M Tris-Cl at pH 7.0 and 7.8, the enzyme was a mixture of oligomers, with species ranging from monomer (47,000 g/mol) to greater than decamer being present. At pH 9.0, only dimer was seen (94,000 g/mol). Large aggregates were present at pH 10.0. The addition of small amounts of KCl, a potent activator of the enzyme, simplified the mixture of oligomers considerably at pH 7.8. A detailed analysis of the data with 0.05 M KCl indicated that dimer and hexamer were the only species present, with marked nonideality. Increasing the KCl concentration to 0.10 M converted all the enzyme to hexamer. The amino acid composition of E. coli citrate synthase was presented. Taken together with peptide mapping experiments of others (J. A. Wright and B. D. Sanwal (1971), J. Biol. Chem. 246 1689), it indicates that the subunits have all the same or very similar amino acid sequences. The dansylation method revealed only methionine at the N-termini of the citrate synthase polypeptide chains. Citrate synthase from E. coli thus resembles the enzyme from eukaryotes in that it consists of subunits weighing just under 50,000 g/mol, although these subunits are more highly aggregated in the bacterial enzyme under most conditions. This conclusion is in disagreement with that of Wright and Sanwal (1971, see above), who reported a subunit size of 62,000 g/mol."}
{"id": "PMID:1091286", "title": "Biosynthesis of bacterial menaquinones. Menaquinone mutants of Escherichia coli.", "content": "The isolation of six menaquinone mutants of Escherichia coli is described. It was shown that the mutants fall into two genetic classes. The first class carries mutations in a gene designated menA, which was located at minute 78 on the E. coli chromosome by cotransduction with the glpK and metB genes. The second class carries mutations in a gene designated menB. It was shown that this gene was not cotransducible with the menA gene. The biosynthesis of menaquinone in E. coli was studied using a variety of mutants blocked in aromatic biosynthesis together with the two classes of menaquinone mutants. It was demonstrated that chorismate is the branch point compound leading to menaquinone, and that 2-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid can serve as menaquinoone precursors in E. coli. It was also shown that menA- and menB- strains accumulate 1,4-dihydroxy-2-naphthoic acid and 2-succinylbenzoic acid, respectively, in their culture supernatants. The accumulation of the two compounds by the mutants together with their activity as menaquinone precursors provide strong evidence that they ar true intermediates in menaquinone biosynthesis. A pathway is proposed for the biosynthesis of bacterial menaquinones in which each intermediate has been adequately characterized.", "contents": "Biosynthesis of bacterial menaquinones. Menaquinone mutants of Escherichia coli. The isolation of six menaquinone mutants of Escherichia coli is described. It was shown that the mutants fall into two genetic classes. The first class carries mutations in a gene designated menA, which was located at minute 78 on the E. coli chromosome by cotransduction with the glpK and metB genes. The second class carries mutations in a gene designated menB. It was shown that this gene was not cotransducible with the menA gene. The biosynthesis of menaquinone in E. coli was studied using a variety of mutants blocked in aromatic biosynthesis together with the two classes of menaquinone mutants. It was demonstrated that chorismate is the branch point compound leading to menaquinone, and that 2-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid can serve as menaquinoone precursors in E. coli. It was also shown that menA- and menB- strains accumulate 1,4-dihydroxy-2-naphthoic acid and 2-succinylbenzoic acid, respectively, in their culture supernatants. The accumulation of the two compounds by the mutants together with their activity as menaquinone precursors provide strong evidence that they ar true intermediates in menaquinone biosynthesis. A pathway is proposed for the biosynthesis of bacterial menaquinones in which each intermediate has been adequately characterized."}
{"id": "PMID:1091287", "title": "Endonuclease II of Escherichia coli: degradation of gammairradiated DNA.", "content": "Irradiation of DNA in a nitrogen atmosphere with 60Co gamma-radiation produces at least two types of damage. The first type leads to single strand breaks in the DNA observed after exposure to alkali. This type of alkali-labile bond will be designated a spontaneous break. The second type of damage to DNA is an alteration which makes the DNA susceptible to phosphodiester bond hydrolysis by a 1600-fold purified preparation of endonuclease II of Escherichia coli and is designated an enzyme-sensitive site. This site is not alkali-labile. After irradiation, preincubation of the DNA either for days at 0 degrees or for 4 hr at 37 degrees increases both the spontaneous breaks and the enzyme sensitive sites. There is a greater increase of spontaneous breaks when the preincubation is in O2 compared to N2. The increase of enzyme sensitive sites due to the preincubation is not altered significantly by O2. The increase of spontaneous breaks during the preincubation is almost completely prevented by addition of either NaBH4 or NH2OH after the irradiation. The treatment can be before or after the preincubation. This effect indicates that these breaks are due to alkali-labile bonds possibly produced by depurination or depyrimidination reactions. That the spontaneous breaks are due primarily to alkali-labile bonds is supported by an experiment in which formamide gradients were used. Neither NaBH4 nor NH2OH has any effect on the enzyme sensitive sites. Addition of beta-mercaptoethanol (0.5 M) at the start of the preincubation prevents in part the appearance of both spontaneous breaks and enzyme-sensitive sites. It has no effect when added at the end of the preincubation. Catalase added before the preincubation has no effect on either type of damage. It is postulated that the spontaneous breaks occur because purine or pyrimidine radicals are formed (possibly hydroxyl radicals) which can then interact with oxygen to produce unstable intermediates. The intermediates then undergo either depurination or depyrimidination. The subsequent alkali catalyzed beta-elimination reaction of depurinated or depyriminidinated DNA is prevented by NaBH4 or NH2OH. An alternative hypothesis would involve damage to the sugar rather than to bases. The enzyme-sensitive sites represent another form of base damage which is not oxygen dependent. The chemical nature of either form of primary damage is not known.", "contents": "Endonuclease II of Escherichia coli: degradation of gammairradiated DNA. Irradiation of DNA in a nitrogen atmosphere with 60Co gamma-radiation produces at least two types of damage. The first type leads to single strand breaks in the DNA observed after exposure to alkali. This type of alkali-labile bond will be designated a spontaneous break. The second type of damage to DNA is an alteration which makes the DNA susceptible to phosphodiester bond hydrolysis by a 1600-fold purified preparation of endonuclease II of Escherichia coli and is designated an enzyme-sensitive site. This site is not alkali-labile. After irradiation, preincubation of the DNA either for days at 0 degrees or for 4 hr at 37 degrees increases both the spontaneous breaks and the enzyme sensitive sites. There is a greater increase of spontaneous breaks when the preincubation is in O2 compared to N2. The increase of enzyme sensitive sites due to the preincubation is not altered significantly by O2. The increase of spontaneous breaks during the preincubation is almost completely prevented by addition of either NaBH4 or NH2OH after the irradiation. The treatment can be before or after the preincubation. This effect indicates that these breaks are due to alkali-labile bonds possibly produced by depurination or depyrimidination reactions. That the spontaneous breaks are due primarily to alkali-labile bonds is supported by an experiment in which formamide gradients were used. Neither NaBH4 nor NH2OH has any effect on the enzyme sensitive sites. Addition of beta-mercaptoethanol (0.5 M) at the start of the preincubation prevents in part the appearance of both spontaneous breaks and enzyme-sensitive sites. It has no effect when added at the end of the preincubation. Catalase added before the preincubation has no effect on either type of damage. It is postulated that the spontaneous breaks occur because purine or pyrimidine radicals are formed (possibly hydroxyl radicals) which can then interact with oxygen to produce unstable intermediates. The intermediates then undergo either depurination or depyrimidination. The subsequent alkali catalyzed beta-elimination reaction of depurinated or depyriminidinated DNA is prevented by NaBH4 or NH2OH. An alternative hypothesis would involve damage to the sugar rather than to bases. The enzyme-sensitive sites represent another form of base damage which is not oxygen dependent. The chemical nature of either form of primary damage is not known."}
{"id": "PMID:1091288", "title": "Transcription of bacteriophage T4 genome in vitro. Heterogeneity of RNA polymerase in crude extracts of normal and T4-infected Escherichia coli B.", "content": "In order to obtain RNA polymerase preparations carrying the necessary specificity determinants to transcribe the delayed-early genes of bacteriophage T4, crude extracts of uninfected and T4-infected Escherichia coli were fractionated in glycerol gradients of low ionic strength. In contrast to the reported sedimentation behavior of the purified enzyme, the RNA polymerase activity in crude extracts of normal and infected cells sedimented heterogeneously over a wide range of sedimentation coefficients. When the \"heavy\" (24-33 S) and \"light\" (14-20 S) regions of the gradient were precipitated with ammonium sulfate and recentrifuged, the former split into two subfractions, one again sedimenting heavy and the other sedimenting light. The latter did not split under the same conditions. The resulting subfractions from uninfected cell extracts had different thermal thermal stabilities at 50 degrees (half-lives ranging from 2-3 to 25 min) while those from T4-infected cell extracts were very thermolabile (half-life of 1-2 min). All the subfractions were more active on T4 DNA than on calf-thymus DNA. They also formed rifampicin-resistant, RNA chain initiation complexes with T4 DNA. Based on the kinetics of heat inactivation with T4 and calf thymus DNAs as templates and preferential transcription of T4 DNA, it is proposed that the T4-infected cell enzymes prepared as described here harbor heat-labile initiation factor(s). During infection the heavy sedimenting RNA polymerase activity disappears after 2.5 min at 37 degrees. This appears to require phage-specific protein synthesis because (a) it does not happen in the presence of chloramphenicol and (b) it does not happen in T4 ghost-infected cells.", "contents": "Transcription of bacteriophage T4 genome in vitro. Heterogeneity of RNA polymerase in crude extracts of normal and T4-infected Escherichia coli B. In order to obtain RNA polymerase preparations carrying the necessary specificity determinants to transcribe the delayed-early genes of bacteriophage T4, crude extracts of uninfected and T4-infected Escherichia coli were fractionated in glycerol gradients of low ionic strength. In contrast to the reported sedimentation behavior of the purified enzyme, the RNA polymerase activity in crude extracts of normal and infected cells sedimented heterogeneously over a wide range of sedimentation coefficients. When the \"heavy\" (24-33 S) and \"light\" (14-20 S) regions of the gradient were precipitated with ammonium sulfate and recentrifuged, the former split into two subfractions, one again sedimenting heavy and the other sedimenting light. The latter did not split under the same conditions. The resulting subfractions from uninfected cell extracts had different thermal thermal stabilities at 50 degrees (half-lives ranging from 2-3 to 25 min) while those from T4-infected cell extracts were very thermolabile (half-life of 1-2 min). All the subfractions were more active on T4 DNA than on calf-thymus DNA. They also formed rifampicin-resistant, RNA chain initiation complexes with T4 DNA. Based on the kinetics of heat inactivation with T4 and calf thymus DNAs as templates and preferential transcription of T4 DNA, it is proposed that the T4-infected cell enzymes prepared as described here harbor heat-labile initiation factor(s). During infection the heavy sedimenting RNA polymerase activity disappears after 2.5 min at 37 degrees. This appears to require phage-specific protein synthesis because (a) it does not happen in the presence of chloramphenicol and (b) it does not happen in T4 ghost-infected cells."}
{"id": "PMID:1091289", "title": "The outer membrane of Proteus mirabilis. I. Isolation and characterization of the outer and cytoplasmic membrane fractions.", "content": "1. The crude envelope preparation obtained by sonication of Proteus mirabilis cells in the presence of lysozyme was separated into outer and cytoplasmic membrane fractions by sucrose density gradient centrifugation. The outer membrane fraction accounted for about two thirds of the dry weight of the envelope preparation. 2. In thin sections, the outer and cytoplasmic membrane fractions were shown to consist of vesicles bounded by a single trilaminar membrane, but those of the outer membrane were considerably smaller and were frequently open, forming C-shaped structures. The cytoplasmic membrane vesicles were cleaved by freeze fracturing to expose fracture faces studded with particles, while the outer membrane fragments resisted cleavage. 3. The outer membrane fraction consisted of protein (similar to 40%), lipopolysaccharide (similar to 36%) and lipid (similar to 18%) and had a density of about 1.22 g/cm3. The cytoplasmic membrane fraction consisted mostly of protein (similar to 56%) and lipid (similar to 38%), had a density of about 1.16 g/cm3, and contained almost all the NADH oxidase, succinate and D-lactate dehydrogenase activities of the crude envelope preparation. 4. Electrophoresis in polyacrylamide gels containing sodium dodecylsulfate revealed over 20 polypeptide bands in the cytoplasmic membrane fraction and only 6-7 in the outer membrane fraction. The outer membrane electrophorogram was dominated by a major band (mol. wt 40 000) which was resolved into two bands when electrophoresed in an acidic gel system. Amino acid analysis revealed a higher content of polar amino acids in the protein moiety of the outer membrane.", "contents": "The outer membrane of Proteus mirabilis. I. Isolation and characterization of the outer and cytoplasmic membrane fractions. 1. The crude envelope preparation obtained by sonication of Proteus mirabilis cells in the presence of lysozyme was separated into outer and cytoplasmic membrane fractions by sucrose density gradient centrifugation. The outer membrane fraction accounted for about two thirds of the dry weight of the envelope preparation. 2. In thin sections, the outer and cytoplasmic membrane fractions were shown to consist of vesicles bounded by a single trilaminar membrane, but those of the outer membrane were considerably smaller and were frequently open, forming C-shaped structures. The cytoplasmic membrane vesicles were cleaved by freeze fracturing to expose fracture faces studded with particles, while the outer membrane fragments resisted cleavage. 3. The outer membrane fraction consisted of protein (similar to 40%), lipopolysaccharide (similar to 36%) and lipid (similar to 18%) and had a density of about 1.22 g/cm3. The cytoplasmic membrane fraction consisted mostly of protein (similar to 56%) and lipid (similar to 38%), had a density of about 1.16 g/cm3, and contained almost all the NADH oxidase, succinate and D-lactate dehydrogenase activities of the crude envelope preparation. 4. Electrophoresis in polyacrylamide gels containing sodium dodecylsulfate revealed over 20 polypeptide bands in the cytoplasmic membrane fraction and only 6-7 in the outer membrane fraction. The outer membrane electrophorogram was dominated by a major band (mol. wt 40 000) which was resolved into two bands when electrophoresed in an acidic gel system. Amino acid analysis revealed a higher content of polar amino acids in the protein moiety of the outer membrane."}
{"id": "PMID:1091291", "title": "Relationships between non-extractable DNA and the bacterial growth cycle.", "content": "Escherichia coli DNA has been fractionated into extractable and non-extractable DNA after deproteinization of detergent-lysed cell preparations with chloroform-isoamyl alcohol. The former was extracted with dilut buffered saline whereas the latter remained in the interphase layer associated with residual cellular debris from which almost 40 percent could be released by incubating with pronase. About 20-25 percent more amino acid residues were bound to the pronase-released DNA than to the extractable DNA, but the relative distribution of the residues in the two DNA samples was virtually identical. The specific activities and the relative amounts of denser (1.709g-cm-3) and lighter (on the surface of CsCl gradients) DNA fractions from E. coli, grown in the presence of labeled thymidine, indicated that these two corresponded to extractable and non-extractable DNA, respectively. The relative amounts of the two fractions varied with the growth phase primarily as a function of the growth rate. Age and metabolic state of cells in the culture or those used as inocula could modify this relative distribution. When growth rate was maximal, the ratio of the two remained at about 1. During lag phase when no appreciable net synthesis of DNA could be detected, there was a rapid and preferential incorporation of labeled thymidine into non-extractable DNA. A disproportionate increase in the fraction of the total DNA which was extractable, was also abserved but only when stationary phase cultures were used as inocula. Complete equilibration of the label in the two DNA fractions was attained only after cultures had reached mid-log phase of growth. Similar results were obtained when prelabeled cells were used. These data have been interpreted as suggesting that the rate of cell growth and DNA synthesis are related to the number or size of sites of attachment of DNA to some cellular structure. Newly synthesized DNA would be attached to different sites on this structure and initiation of DNA replication in lag phase would require reorientation of the two kinds of DNA. Small peptides which are firmly bound to the DNA and which vary quantitatively with the rate of DNA synthesis could perhaps be involved in the attachment to the sites.", "contents": "Relationships between non-extractable DNA and the bacterial growth cycle. Escherichia coli DNA has been fractionated into extractable and non-extractable DNA after deproteinization of detergent-lysed cell preparations with chloroform-isoamyl alcohol. The former was extracted with dilut buffered saline whereas the latter remained in the interphase layer associated with residual cellular debris from which almost 40 percent could be released by incubating with pronase. About 20-25 percent more amino acid residues were bound to the pronase-released DNA than to the extractable DNA, but the relative distribution of the residues in the two DNA samples was virtually identical. The specific activities and the relative amounts of denser (1.709g-cm-3) and lighter (on the surface of CsCl gradients) DNA fractions from E. coli, grown in the presence of labeled thymidine, indicated that these two corresponded to extractable and non-extractable DNA, respectively. The relative amounts of the two fractions varied with the growth phase primarily as a function of the growth rate. Age and metabolic state of cells in the culture or those used as inocula could modify this relative distribution. When growth rate was maximal, the ratio of the two remained at about 1. During lag phase when no appreciable net synthesis of DNA could be detected, there was a rapid and preferential incorporation of labeled thymidine into non-extractable DNA. A disproportionate increase in the fraction of the total DNA which was extractable, was also abserved but only when stationary phase cultures were used as inocula. Complete equilibration of the label in the two DNA fractions was attained only after cultures had reached mid-log phase of growth. Similar results were obtained when prelabeled cells were used. These data have been interpreted as suggesting that the rate of cell growth and DNA synthesis are related to the number or size of sites of attachment of DNA to some cellular structure. Newly synthesized DNA would be attached to different sites on this structure and initiation of DNA replication in lag phase would require reorientation of the two kinds of DNA. Small peptides which are firmly bound to the DNA and which vary quantitatively with the rate of DNA synthesis could perhaps be involved in the attachment to the sites."}
{"id": "PMID:1091292", "title": "Aminoacylation of tRNA-Leu species from Escherichia coli and from the cytoplasm, chloroplasts and mitochondria of Phaseolus vulgaris by homologous and heterologous enzymes.", "content": "Leucyl-tRNA synthetase from Phaseolus vulgaris chloroplasts could be separated from its cytoplasmic counterpart upon chromatography on hydroxyapatite, but the cytoplasmic and mitochondrial leucyl-tRNA synthetases could not be distinguished. The tRNALeu species from the various plant cell compartments and from Escherichia coli were aminoacylated using either homologous or heterologous enzymes; the levels of aminoacylation and the profiles of the leucyl-tRNAs upon reverse-phase chromatography were studied. Cytoplasmic tRNALeu species could be aminoacylated by the cytoplasmic or by the mitochondrial enzymes and in both cases yielded two peaks upon reverse-phase chromatography (RPC-5). But they could not be charged by the chloroplast-specific or by the E. coli enzynes. Mitochondrial tRNALeu species could be charged by the mitochondrial or by the cytoplasmic enzymes and in both cases yielded four peaks upon reverse phase (RPC-5) chromatography. But they could not be aminoacylated using the chloroplast-specific or the E. coli leucyl-tRNA synthetases. Chloroplastic tRNALeu species can be divided into two classes: the first class contains four isoacceptor species which can be charged by the cytoplasmic or mitochondrial enzymes, but not by the chloroplast-specific or the E. coli enzymes; the second class contains three chloroplast-specific tRNALeu species which can be charged by the chloroplast-specific or the E. coli enzymes but not by the cytoplasmic or the mitochondrial enzymes. There are five isoacceptor tRNALeu species in E. coli; all are charged by the E. coli or the chloroplast-specific enzymes, while only one is aminoacylated by the plant cytoplasmic or mitochondrial enzymes.", "contents": "Aminoacylation of tRNA-Leu species from Escherichia coli and from the cytoplasm, chloroplasts and mitochondria of Phaseolus vulgaris by homologous and heterologous enzymes. Leucyl-tRNA synthetase from Phaseolus vulgaris chloroplasts could be separated from its cytoplasmic counterpart upon chromatography on hydroxyapatite, but the cytoplasmic and mitochondrial leucyl-tRNA synthetases could not be distinguished. The tRNALeu species from the various plant cell compartments and from Escherichia coli were aminoacylated using either homologous or heterologous enzymes; the levels of aminoacylation and the profiles of the leucyl-tRNAs upon reverse-phase chromatography were studied. Cytoplasmic tRNALeu species could be aminoacylated by the cytoplasmic or by the mitochondrial enzymes and in both cases yielded two peaks upon reverse-phase chromatography (RPC-5). But they could not be charged by the chloroplast-specific or by the E. coli enzynes. Mitochondrial tRNALeu species could be charged by the mitochondrial or by the cytoplasmic enzymes and in both cases yielded four peaks upon reverse phase (RPC-5) chromatography. But they could not be aminoacylated using the chloroplast-specific or the E. coli leucyl-tRNA synthetases. Chloroplastic tRNALeu species can be divided into two classes: the first class contains four isoacceptor species which can be charged by the cytoplasmic or mitochondrial enzymes, but not by the chloroplast-specific or the E. coli enzymes; the second class contains three chloroplast-specific tRNALeu species which can be charged by the chloroplast-specific or the E. coli enzymes but not by the cytoplasmic or the mitochondrial enzymes. There are five isoacceptor tRNALeu species in E. coli; all are charged by the E. coli or the chloroplast-specific enzymes, while only one is aminoacylated by the plant cytoplasmic or mitochondrial enzymes."}
{"id": "PMID:1091293", "title": "Escherichia coli mutants deficient in RNA accumulation at high temperature.", "content": "A selection technique is described which has permitted isolation of 10 mutants that continue to form protein, but are deficient in the accumulation of rRNA at 42 degrees C. DNA-RNA hybridization experiments have demonstrated that most of these mutants are specifically defective in their ability to synthesize rRNA at the restrictive temperature. The bulk of the pulse-labeled RNA formed in such mutants at 42 degrees C appears to be mRNA, with normal instability as measured in the presence of rifampicin. The remaining mutants appear to synthesize normal levels of rRNA, but that rRNA does not accumulate in a stable form. Since all of these mutants continue to form protein, and most do not accumulate significant levels of ppGpp at 42 degrees C, it appears likely that the shut-off of rRNA synthesis at 42 degrees C does not act through a lesion in the rel locus. Thus, these mutants may reveal another element(s) required to promote ribosomal RNA formation.", "contents": "Escherichia coli mutants deficient in RNA accumulation at high temperature. A selection technique is described which has permitted isolation of 10 mutants that continue to form protein, but are deficient in the accumulation of rRNA at 42 degrees C. DNA-RNA hybridization experiments have demonstrated that most of these mutants are specifically defective in their ability to synthesize rRNA at the restrictive temperature. The bulk of the pulse-labeled RNA formed in such mutants at 42 degrees C appears to be mRNA, with normal instability as measured in the presence of rifampicin. The remaining mutants appear to synthesize normal levels of rRNA, but that rRNA does not accumulate in a stable form. Since all of these mutants continue to form protein, and most do not accumulate significant levels of ppGpp at 42 degrees C, it appears likely that the shut-off of rRNA synthesis at 42 degrees C does not act through a lesion in the rel locus. Thus, these mutants may reveal another element(s) required to promote ribosomal RNA formation."}
{"id": "PMID:1091294", "title": "Contractile proteins. Major components of nuclear and chromosome non-histone proteins.", "content": "Two of the major non-histone proteins from Physarum polycephalum have been isolated under nondenaturing conditions and identified as actin and myosin. A third protein has been purified from crude nuclear actomyosin and from residual nonhistone fractions and found to bind actomyosin in the presence of Mg2+. In Physarum these proteins are not components of the nuclear membrane. Based on sodium dodecylsulfate-polyacrylamide gel electrophoresis, similar proteins are also present in nuclei of HeLa cells and mouse embryo fibroblasts. Isolated metaphase chromosomes from Physarum show a several-fold enrichment in myosin and an altered ratio of actin to the Mg2+-dependent actomyosin binding protein as compared to interphase nuclei. When non-proliferative states are induced in any of these cells, the Mg2+-dependent actomyosin binding protein decreases while actin increases several fold in intranuclear concentration; concomitantly, there is a generalized condensation and inactivation of chromatin. Experiments with added purified radioactive nuclear actomyosin; comparative studies on nuclear protein during stepwise nuclear purification; and studies on isolated metaphase chromosomes indicate that these proteins exist in nuclei in vivo. These observations suggest that contractile proteins may function in the structural interconversions of chromatin and in the regulation of cell proliferation;", "contents": "Contractile proteins. Major components of nuclear and chromosome non-histone proteins. Two of the major non-histone proteins from Physarum polycephalum have been isolated under nondenaturing conditions and identified as actin and myosin. A third protein has been purified from crude nuclear actomyosin and from residual nonhistone fractions and found to bind actomyosin in the presence of Mg2+. In Physarum these proteins are not components of the nuclear membrane. Based on sodium dodecylsulfate-polyacrylamide gel electrophoresis, similar proteins are also present in nuclei of HeLa cells and mouse embryo fibroblasts. Isolated metaphase chromosomes from Physarum show a several-fold enrichment in myosin and an altered ratio of actin to the Mg2+-dependent actomyosin binding protein as compared to interphase nuclei. When non-proliferative states are induced in any of these cells, the Mg2+-dependent actomyosin binding protein decreases while actin increases several fold in intranuclear concentration; concomitantly, there is a generalized condensation and inactivation of chromatin. Experiments with added purified radioactive nuclear actomyosin; comparative studies on nuclear protein during stepwise nuclear purification; and studies on isolated metaphase chromosomes indicate that these proteins exist in nuclei in vivo. These observations suggest that contractile proteins may function in the structural interconversions of chromatin and in the regulation of cell proliferation;"}
{"id": "PMID:1091295", "title": "13C-enriched phosphatidylethanolamines from Escherichia coli.", "content": "Escherichia coli have been grown using [1-13C]acetate and [2-13C]acetate as the sole carbon source. The 13C NMR spectra of the whole cells and spheroplasts can be readily obtained but give limited information. The 13C NMR spectra of t-e isolated 13C-enriched phosphatidylethanolamines are assigned and analysed to give the biosynthetic pathway for acetate incorporation. This method provides a ready source of 13C-enriched phospholipids.", "contents": "13C-enriched phosphatidylethanolamines from Escherichia coli. Escherichia coli have been grown using [1-13C]acetate and [2-13C]acetate as the sole carbon source. The 13C NMR spectra of the whole cells and spheroplasts can be readily obtained but give limited information. The 13C NMR spectra of t-e isolated 13C-enriched phosphatidylethanolamines are assigned and analysed to give the biosynthetic pathway for acetate incorporation. This method provides a ready source of 13C-enriched phospholipids."}
{"id": "PMID:1091296", "title": "Uridine diphosphate 2-deoxyglucose. Chemical synthesis, enzymic oxidation and epimerization.", "content": "The paper describes chemical synthesis of uridine diphosphate 2-deocyglucose (UDPdGLc) through reaction of uridine 5'-phosphomorpholidate with 2-deoxy-a-D-glucopyranosyl phosphate. The prepared analog of uridine diphosphate glucose (UDPGlc) served as a substrate for calf liver UDPGlc dehydrogenases (EC 1.1.1.22), the reaction product was identified as nucleotide deoxyhexuronic acid derivative. The apparent Km for UDPdGlc was found to be 60 times that of UDPGlc, and the relative V value for the analog was 0.09. The peculiar lag-eriod in reaction kinetics has been observed for the analog and is presumably connected with the slow rate of the initial stages of the reaction. UDPdGlc was found to be quite an efficient substrate for UDPGlc 4-epimerases (EC 5.13.2) from yeast, calf liver and mung bean seedlings.", "contents": "Uridine diphosphate 2-deoxyglucose. Chemical synthesis, enzymic oxidation and epimerization. The paper describes chemical synthesis of uridine diphosphate 2-deocyglucose (UDPdGLc) through reaction of uridine 5'-phosphomorpholidate with 2-deoxy-a-D-glucopyranosyl phosphate. The prepared analog of uridine diphosphate glucose (UDPGlc) served as a substrate for calf liver UDPGlc dehydrogenases (EC 1.1.1.22), the reaction product was identified as nucleotide deoxyhexuronic acid derivative. The apparent Km for UDPdGlc was found to be 60 times that of UDPGlc, and the relative V value for the analog was 0.09. The peculiar lag-eriod in reaction kinetics has been observed for the analog and is presumably connected with the slow rate of the initial stages of the reaction. UDPdGlc was found to be quite an efficient substrate for UDPGlc 4-epimerases (EC 5.13.2) from yeast, calf liver and mung bean seedlings."}
{"id": "PMID:1091297", "title": "Alteration of regulation of arginine biosynthesis in Escherichia coli W by mutation to rifampin resistance.", "content": "The synthesis of acetylornithine delta-transaminase is induced by arginine and by rifampin in an arginine-inducible mutant of Escherichia coli W. A mutant of the arginine-inducible strain was isolated which is resistant to rifampin. The mutation which has brought about rifampin resistance has altered RNA polymerase and has simultaneously altered the regulation of arginine biosynthesis. Three of the enzymes of arginine biosynthesis, acetylornithinase, ornithine transcarbamylase, and argininosuccinase, show a greater rate of derepression and a 2--12-fold higher level of enzyme activity in the rifampin-resistant mutant than in the parent arginine-inducible strain. Acetylornithine delta-transaminase is no longer inducible by rifampin alone, and the level of inducibility by arginine plus rifampin has been reduced by 70%. The results indicate that RNA polymerase is involved in regulation of arginine biosynthesis in E. coli W.", "contents": "Alteration of regulation of arginine biosynthesis in Escherichia coli W by mutation to rifampin resistance. The synthesis of acetylornithine delta-transaminase is induced by arginine and by rifampin in an arginine-inducible mutant of Escherichia coli W. A mutant of the arginine-inducible strain was isolated which is resistant to rifampin. The mutation which has brought about rifampin resistance has altered RNA polymerase and has simultaneously altered the regulation of arginine biosynthesis. Three of the enzymes of arginine biosynthesis, acetylornithinase, ornithine transcarbamylase, and argininosuccinase, show a greater rate of derepression and a 2--12-fold higher level of enzyme activity in the rifampin-resistant mutant than in the parent arginine-inducible strain. Acetylornithine delta-transaminase is no longer inducible by rifampin alone, and the level of inducibility by arginine plus rifampin has been reduced by 70%. The results indicate that RNA polymerase is involved in regulation of arginine biosynthesis in E. coli W."}
{"id": "PMID:1091298", "title": "Purification of an inhibitor of DNA photolyase with fluorescent spectra similar to those of the enzyme.", "content": "1. The binding of DNA photolyase, the enzyme which uncouples cyclobutadipyrimidines in DNA upon illumination, to its substrate was enhanced by a substance isolated from acidified autolysates of baker's yeast. 2. This substance, referred to as activator, was partially purified by chloroform extraction, ion-exchange chromatography on Dowex 50 and DEAE-cellulose, and gel filtration on Sephadex G-10. Upon lyophilization and storage at -20 degrees C, it was converted to a potent inhibitor of enzyme binding to substrate. 3. The inhibitor appeared as a single band in two thin-layer chromatography systems and was detected by ultraviolet absorbance, fluorescence, and ninhydrin staining. 4. The similarity between the fluorescence spectra of the inhibitor and the enzyme suggested that the inhibitor was structurally analogous to a chromophore in the enzyme and that the activator from which the inhibitor was derived may be the active chromophore of the enzyme.", "contents": "Purification of an inhibitor of DNA photolyase with fluorescent spectra similar to those of the enzyme. 1. The binding of DNA photolyase, the enzyme which uncouples cyclobutadipyrimidines in DNA upon illumination, to its substrate was enhanced by a substance isolated from acidified autolysates of baker's yeast. 2. This substance, referred to as activator, was partially purified by chloroform extraction, ion-exchange chromatography on Dowex 50 and DEAE-cellulose, and gel filtration on Sephadex G-10. Upon lyophilization and storage at -20 degrees C, it was converted to a potent inhibitor of enzyme binding to substrate. 3. The inhibitor appeared as a single band in two thin-layer chromatography systems and was detected by ultraviolet absorbance, fluorescence, and ninhydrin staining. 4. The similarity between the fluorescence spectra of the inhibitor and the enzyme suggested that the inhibitor was structurally analogous to a chromophore in the enzyme and that the activator from which the inhibitor was derived may be the active chromophore of the enzyme."}
{"id": "PMID:1091299", "title": "Action of exonuclease V (the recBC enzyme) on ultraviolet-irradiated DNA.", "content": "Exonuclease V (the recBC enzyme) of Escherichia coli can release pyrimidine dimers from ultraviolet-irradiated linear duplex DNA though it acts more slowly on irradiated DNA than on non-irradiated DAN. However, close circular lambda-dv DNA or phi X174 replicative form I DNA is not attacked by exonuclease V even though the DNA has been irradiated and treated with T4 endonuclease V to produce single-stranded breaks at the 5'-side of pyrimidine dimers. When irradiated circular DNA, previously nicked by T4 endonuclease V, is briefly exposed to elevated temperature, the DAN becomes susceptible to the action of exonuclease V, and pyrimidine dimers are selectively released. The increased susceptibility to exonuclease V may be resulted from locarized denaturation, or \"fraying\" of the 5'-termini at the nicks. The preferential release of pyrimidine dimers was observed when irradiated DNA, treated with T4 endonuclease V, was incubated with crude extracts of Escherichia coli. The activity was found in various strains defective in exonuclease V and/or DNA polymerase I.", "contents": "Action of exonuclease V (the recBC enzyme) on ultraviolet-irradiated DNA. Exonuclease V (the recBC enzyme) of Escherichia coli can release pyrimidine dimers from ultraviolet-irradiated linear duplex DNA though it acts more slowly on irradiated DNA than on non-irradiated DAN. However, close circular lambda-dv DNA or phi X174 replicative form I DNA is not attacked by exonuclease V even though the DNA has been irradiated and treated with T4 endonuclease V to produce single-stranded breaks at the 5'-side of pyrimidine dimers. When irradiated circular DNA, previously nicked by T4 endonuclease V, is briefly exposed to elevated temperature, the DAN becomes susceptible to the action of exonuclease V, and pyrimidine dimers are selectively released. The increased susceptibility to exonuclease V may be resulted from locarized denaturation, or \"fraying\" of the 5'-termini at the nicks. The preferential release of pyrimidine dimers was observed when irradiated DNA, treated with T4 endonuclease V, was incubated with crude extracts of Escherichia coli. The activity was found in various strains defective in exonuclease V and/or DNA polymerase I."}
{"id": "PMID:1091300", "title": "Excision of pyrimidine dimers in normal and T4-infected Escherichia coli: effect of polA and other mutations.", "content": "Strains carrying both polA1 and recBts1 mutations, which are defective in DNA polymerase I and have thermolabile exonuclease V (the recBC enzyme), are viable at 30 degrees C but not at 42 degrees C. These mutants exhibit almost normal rate of dimer excision in vivo even at the restrictive temperature. Similar results were obtained with other polA minus strains. We have also investigated effect of host and phage mutations on excision of dimers in T4-infected cells. Only a small amount of dimer is excised in T4v-1-infected cells whereas an extensive and selective release of dimers takes place in T4D-infected cells. Other phage mutations, including mutations in gene 43 and gene 30, do not affect excision of dimers in infected cells.", "contents": "Excision of pyrimidine dimers in normal and T4-infected Escherichia coli: effect of polA and other mutations. Strains carrying both polA1 and recBts1 mutations, which are defective in DNA polymerase I and have thermolabile exonuclease V (the recBC enzyme), are viable at 30 degrees C but not at 42 degrees C. These mutants exhibit almost normal rate of dimer excision in vivo even at the restrictive temperature. Similar results were obtained with other polA minus strains. We have also investigated effect of host and phage mutations on excision of dimers in T4-infected cells. Only a small amount of dimer is excised in T4v-1-infected cells whereas an extensive and selective release of dimers takes place in T4D-infected cells. Other phage mutations, including mutations in gene 43 and gene 30, do not affect excision of dimers in infected cells."}
{"id": "PMID:1091301", "title": "Cell division in Agmenellium quadruplicatum: evidence for the negative control by a protein.", "content": "A high temperature conditional snake mutant, strain D1, of Agmenellum quadruplicatum was isolated which immediately stopped dividing following a shift to 41 degrees C following treatment with nitrosoguanidine. This mutant was stimulated to divide at 41 degrees C by the addition of inhibitors of RNA or protein synthesis: rifampicin, streptomycin, puromycin and chloramphenicol. Each of these inhibitors exhibited a discrete concentration optimum. The optimal concentration of chloramphenicol for cell division corresponded to the minimal concentrations necessary for the rapid inhibition of protein synthesis. The ability of chloramphenicol and other inhibitors to induce cell division in filaments decayed rapidly upon shifting to 30 degrees C. These results are interpreted as evidence for a protein acting as a negative regulator late in the cell cycle. At 41 degrees C, DNA was found distributed as a continuous zone throughout the length of the filaments. The addition of inhibitors of protein or RNA synthesis resulted in a rapid condensation of this nuclear material into multiple discrete nuclear regions suggesting that the negative control may be at the level of nuclear compartmentalization.", "contents": "Cell division in Agmenellium quadruplicatum: evidence for the negative control by a protein. A high temperature conditional snake mutant, strain D1, of Agmenellum quadruplicatum was isolated which immediately stopped dividing following a shift to 41 degrees C following treatment with nitrosoguanidine. This mutant was stimulated to divide at 41 degrees C by the addition of inhibitors of RNA or protein synthesis: rifampicin, streptomycin, puromycin and chloramphenicol. Each of these inhibitors exhibited a discrete concentration optimum. The optimal concentration of chloramphenicol for cell division corresponded to the minimal concentrations necessary for the rapid inhibition of protein synthesis. The ability of chloramphenicol and other inhibitors to induce cell division in filaments decayed rapidly upon shifting to 30 degrees C. These results are interpreted as evidence for a protein acting as a negative regulator late in the cell cycle. At 41 degrees C, DNA was found distributed as a continuous zone throughout the length of the filaments. The addition of inhibitors of protein or RNA synthesis resulted in a rapid condensation of this nuclear material into multiple discrete nuclear regions suggesting that the negative control may be at the level of nuclear compartmentalization."}
{"id": "PMID:1091305", "title": "A double-blind study of electrosleep for anxiety and insomnia.", "content": "Despite largely negative findings, several subjects reported a remarkable improvement in their symptoms some two to three weeks after electrosleep (ES) treatment was concluded, so that it remains unclear whether or not ES may be an effective treatment. The clinical experience reported suggests that five half-hour ES treatments may not be sufficient to produce significant changes in the patient's anxiety and insomnia. Further investigations are required to examine the effects of varying durations of treatment.", "contents": "A double-blind study of electrosleep for anxiety and insomnia. Despite largely negative findings, several subjects reported a remarkable improvement in their symptoms some two to three weeks after electrosleep (ES) treatment was concluded, so that it remains unclear whether or not ES may be an effective treatment. The clinical experience reported suggests that five half-hour ES treatments may not be sufficient to produce significant changes in the patient's anxiety and insomnia. Further investigations are required to examine the effects of varying durations of treatment."}
{"id": "PMID:1091307", "title": "Light-scattering study of the temperature dependence of Escherichia coli motility.", "content": "Two light-scattering techniques are used to study the temperature dependence of translational and rotational motility in Escherichia coli. The method of number fluctuation spectroscopy is developed theoretically and experimentally to measure the translational swimming speed of a smooth swimming strain of E. coli. Interference fluctuation techniques are used to study the rotational component of the motion. The results demonstrate that the thrust remains proportional the the torque generated by the flagella throughout the range studied and also show that relative changes in translational swimming speed may be inferred from the dynamics of rotational motion.", "contents": "Light-scattering study of the temperature dependence of Escherichia coli motility. Two light-scattering techniques are used to study the temperature dependence of translational and rotational motility in Escherichia coli. The method of number fluctuation spectroscopy is developed theoretically and experimentally to measure the translational swimming speed of a smooth swimming strain of E. coli. Interference fluctuation techniques are used to study the rotational component of the motion. The results demonstrate that the thrust remains proportional the the torque generated by the flagella throughout the range studied and also show that relative changes in translational swimming speed may be inferred from the dynamics of rotational motion."}
{"id": "PMID:1091308", "title": "Adverse effects of intrathecal methotrexate in children with acute leukemia in remission.", "content": "A toxic syndrome characterized by fever, headache, and vomiting, lasting 2-5 days, occurred in 61% of 39 children with acute leukemia in complete remission, receiving central nervous system prophylaxis with intrathecal methotrexate, and in 14% of 34 children receiving the same plus cranial radiation. The syndrome was accompanied by pleocytosis with lymphocytes, monocytoid cells, and neutrophils. There was evidence of cumulative Mtx toxicity, since the toxic syndrome occurred mostly after the third and fourth dose and did not recur with longer intervals between doses. The incidence of the syndrome was significantly reduced by the use of Elliott's B solution as Mtx diluent, rather than water or normal saline. The occurrence of pleocytosis and toxic clinical syndrome was also significantly reduced in patients receiving concomitant cranial radiation, probably due to the lympholytic action of radiotherapy and the depressed cellular response of irradiated tissues. The use of Elliott's B solution as diluent for IT Mtx and an appropriate interval between Mtx doses are suggested for prevention of this toxic syndrome.", "contents": "Adverse effects of intrathecal methotrexate in children with acute leukemia in remission. A toxic syndrome characterized by fever, headache, and vomiting, lasting 2-5 days, occurred in 61% of 39 children with acute leukemia in complete remission, receiving central nervous system prophylaxis with intrathecal methotrexate, and in 14% of 34 children receiving the same plus cranial radiation. The syndrome was accompanied by pleocytosis with lymphocytes, monocytoid cells, and neutrophils. There was evidence of cumulative Mtx toxicity, since the toxic syndrome occurred mostly after the third and fourth dose and did not recur with longer intervals between doses. The incidence of the syndrome was significantly reduced by the use of Elliott's B solution as Mtx diluent, rather than water or normal saline. The occurrence of pleocytosis and toxic clinical syndrome was also significantly reduced in patients receiving concomitant cranial radiation, probably due to the lympholytic action of radiotherapy and the depressed cellular response of irradiated tissues. The use of Elliott's B solution as diluent for IT Mtx and an appropriate interval between Mtx doses are suggested for prevention of this toxic syndrome."}
{"id": "PMID:1091309", "title": "Effect of bleeding on hematopoiesis following irradiation and marrow transplantation.", "content": "In previous studies, bleeding after irradiation did not affect the rate of regeneration of endogenous spleen colony-forming cells, but induced an early (4-6 days after irradiation) appearance of erythrocytic colonies which differentiated and disappeared by days 7-8. This \"abortive\" wave was associated with a similarly abortive wave of splenic 59Fe uptake. The present experiments were done to determine whether or not an abortive wave of erythropoiesis could be induced in the transplanted, exogenous stem cell system. Lethally irradiated mice were given normal bone marrow cells and one-half of the group were bled of about one-third their blood volume within 4 hr of irradiation. Groups were killed on days 3-10 after irradiation. Seventeen to twenty hours prior to killing, 59Fe was injected. Hematocrits, spleen weights, colony numbers, and per cent 59Fe uptake were determined. Hematocrits of bled mice averaged about 70% of those of cell-injected controls. Spleen weights, colony counts, and per cent 59Fe uptake per spleen began to increase about 1 day earlier in bled mice (days 4-5 as compared to days 5-6), and rates of increase were the same as those of controls. However, no abortive wave of erythropoiesis was detected. A large cell dose resulted in earlier increases in all parameters than a small dose. Thus, bleeding after injection of cells produced results similar to those obtained by increasing the cell dose. The inability of bleeding to induce an early abortive wave of erythropoiesis in transplanted as compared to endogenous colony-forming systems may reflect differences in the cell cycling characteristics of these systems.", "contents": "Effect of bleeding on hematopoiesis following irradiation and marrow transplantation. In previous studies, bleeding after irradiation did not affect the rate of regeneration of endogenous spleen colony-forming cells, but induced an early (4-6 days after irradiation) appearance of erythrocytic colonies which differentiated and disappeared by days 7-8. This \"abortive\" wave was associated with a similarly abortive wave of splenic 59Fe uptake. The present experiments were done to determine whether or not an abortive wave of erythropoiesis could be induced in the transplanted, exogenous stem cell system. Lethally irradiated mice were given normal bone marrow cells and one-half of the group were bled of about one-third their blood volume within 4 hr of irradiation. Groups were killed on days 3-10 after irradiation. Seventeen to twenty hours prior to killing, 59Fe was injected. Hematocrits, spleen weights, colony numbers, and per cent 59Fe uptake were determined. Hematocrits of bled mice averaged about 70% of those of cell-injected controls. Spleen weights, colony counts, and per cent 59Fe uptake per spleen began to increase about 1 day earlier in bled mice (days 4-5 as compared to days 5-6), and rates of increase were the same as those of controls. However, no abortive wave of erythropoiesis was detected. A large cell dose resulted in earlier increases in all parameters than a small dose. Thus, bleeding after injection of cells produced results similar to those obtained by increasing the cell dose. The inability of bleeding to induce an early abortive wave of erythropoiesis in transplanted as compared to endogenous colony-forming systems may reflect differences in the cell cycling characteristics of these systems."}
{"id": "PMID:1091310", "title": "The role of granulocytes in the activation of intravascular coagulation and the precipitation of soluble fibrin by endotoxin.", "content": "This study examines the role of neutrophils (PMN) in the pathogenesis of endotoxin-induced microclot formation. It is intended to clarify whether granulocytes are involved in endotoxin-induced activation of intravascular coagulation (generation of soluble fibrin) and/or in endotoxin-induced precipitation of soluble fibrin. Precipitation of soluble fibrin was achieved by injection of endotoxin into ancrod-infused rabbits with circulating soluble fibrin (first model). Activation of intravascular coagulation was elicited by two intravenous injections of endotoxin into rabbits (second model). Seventy-two and ninety-six hours after injection of nitrogen mustard, leukopenic rabbits had PMN counts between 0 and 50 cells per mul. Neutropenia did not prevent the occurrence of glomerular microclots after infusion of ancrod and injection of endotoxin (first model). Neutropenia influenced neither the decrease in mean fibrinogen concentrations nor the drop in mean platelet counts after ancrod and endotoxin administration. In contrast to the first model, neutropenia prevented the occurrence of glomerular microclots and of circulating soluble fibrin after two injections of endotoxin (second model). It did not, however, protect rabbits from the decrease in mean platelet counts after endotoxin administration. These data indicate that granulocytes are involved in endotoxin-induced activation of intravascular coagulation and the production of soluble fibrin but are not essential to endotoxin-induced precipitation of soluble fibrin.", "contents": "The role of granulocytes in the activation of intravascular coagulation and the precipitation of soluble fibrin by endotoxin. This study examines the role of neutrophils (PMN) in the pathogenesis of endotoxin-induced microclot formation. It is intended to clarify whether granulocytes are involved in endotoxin-induced activation of intravascular coagulation (generation of soluble fibrin) and/or in endotoxin-induced precipitation of soluble fibrin. Precipitation of soluble fibrin was achieved by injection of endotoxin into ancrod-infused rabbits with circulating soluble fibrin (first model). Activation of intravascular coagulation was elicited by two intravenous injections of endotoxin into rabbits (second model). Seventy-two and ninety-six hours after injection of nitrogen mustard, leukopenic rabbits had PMN counts between 0 and 50 cells per mul. Neutropenia did not prevent the occurrence of glomerular microclots after infusion of ancrod and injection of endotoxin (first model). Neutropenia influenced neither the decrease in mean fibrinogen concentrations nor the drop in mean platelet counts after ancrod and endotoxin administration. In contrast to the first model, neutropenia prevented the occurrence of glomerular microclots and of circulating soluble fibrin after two injections of endotoxin (second model). It did not, however, protect rabbits from the decrease in mean platelet counts after endotoxin administration. These data indicate that granulocytes are involved in endotoxin-induced activation of intravascular coagulation and the production of soluble fibrin but are not essential to endotoxin-induced precipitation of soluble fibrin."}
{"id": "PMID:1091311", "title": "[Nitroblue-tetrazolium test in isolated human monocytes].", "content": "Depending upon the NBT charge 50 to 80% of isolated non stimulated human monocytes reduce NBT ro morphologically demonstrable Formazan. Similar to neutrophils two different patterns of reaction are exhibited: discrete load with finely distributed Formazan granula surrounding the nucleus; massive reaction with large distributed Formazan granula surrounding the nucleus; massive reaction with large Formazan deposits throughout the cytoplasma. In a low percentage morphologically desintegrated, massive loaden cells appear. After an additional incubation in medium without NBT the number of these necrotic cells increases. The phagocytosis of IgG-coated red cells is impaired after incubation of the monocytes in NBT. In the presence of an alkylating agent not only the NBT-reaction is inhibited; the number of necrotic cells is also diminished. Apparently the Formazan is cytotoxic. The significance of these results in particular in cases of enhanced NBT reduction is discussed. Compared with the findings of other authors concerning the neutrophils the monocytes possess a markedly stronger NBT reducing activity. Considering the lower bactericidal capacity of the monocytes NBT reduction does not in every case parallel bactericidal activity.", "contents": "[Nitroblue-tetrazolium test in isolated human monocytes]. Depending upon the NBT charge 50 to 80% of isolated non stimulated human monocytes reduce NBT ro morphologically demonstrable Formazan. Similar to neutrophils two different patterns of reaction are exhibited: discrete load with finely distributed Formazan granula surrounding the nucleus; massive reaction with large distributed Formazan granula surrounding the nucleus; massive reaction with large Formazan deposits throughout the cytoplasma. In a low percentage morphologically desintegrated, massive loaden cells appear. After an additional incubation in medium without NBT the number of these necrotic cells increases. The phagocytosis of IgG-coated red cells is impaired after incubation of the monocytes in NBT. In the presence of an alkylating agent not only the NBT-reaction is inhibited; the number of necrotic cells is also diminished. Apparently the Formazan is cytotoxic. The significance of these results in particular in cases of enhanced NBT reduction is discussed. Compared with the findings of other authors concerning the neutrophils the monocytes possess a markedly stronger NBT reducing activity. Considering the lower bactericidal capacity of the monocytes NBT reduction does not in every case parallel bactericidal activity."}
{"id": "PMID:1091319", "title": "Assessing psychological conflict.", "content": "A method was constructed to assess cognitive conflict. It uses a large number of a subject's beliefs and attitudes which may be connected with his personal problems. The subject rates the direction and the degree of these beliefs and attitudes and the proportion of conflict involved in them is then calculated by computer according to a model basically derived from Heider's cognitive balance theory.", "contents": "Assessing psychological conflict. A method was constructed to assess cognitive conflict. It uses a large number of a subject's beliefs and attitudes which may be connected with his personal problems. The subject rates the direction and the degree of these beliefs and attitudes and the proportion of conflict involved in them is then calculated by computer according to a model basically derived from Heider's cognitive balance theory."}
{"id": "PMID:1091321", "title": "Single layer anastomosis after rectosigmoid resection.", "content": "Anastomosis after resection of the rectosigmoid colon is often followed by leakage at the suture line. When occult leakage discovered on routine contrast study is included incidence rates of over 50 per cent have recently been reported. A single layer interrupted seromuscular inverting technique was used for the anastomosis after elective rectosigmoid resection in 52 consecutive patients. The total incidence of suture line leakage was 6 per cent. Therefore a high incidence of anastomotic dehiscence in the rectum can be avoided and it is concluded that a single layer technique is sound.", "contents": "Single layer anastomosis after rectosigmoid resection. Anastomosis after resection of the rectosigmoid colon is often followed by leakage at the suture line. When occult leakage discovered on routine contrast study is included incidence rates of over 50 per cent have recently been reported. A single layer interrupted seromuscular inverting technique was used for the anastomosis after elective rectosigmoid resection in 52 consecutive patients. The total incidence of suture line leakage was 6 per cent. Therefore a high incidence of anastomotic dehiscence in the rectum can be avoided and it is concluded that a single layer technique is sound."}
{"id": "PMID:1091333", "title": "Selective labelling ot the methyl carboxylate substituents found in the anticodon sequences of some species of yeast transfer RNA.", "content": "(1) By incubation in 0.1 M NaOH for 10 min at room temperature, it is possible to \"saponify\" some of the methyl carboxylate linkages in bulk yeast tRNA. By incubation with S-adenosyl(Me-14-C)methionine and either homologous (yeast) or heterologous (wheat-embryo) enzymes, it is then possible to \"re-esterify\" the \"saponified\" tRNA and thereby effect selective labelling at 5-carboxymethyluridine (Me-14-C)methyl ester residues. (2) There is also selective labelling at 2-thio-5-carboxymethyluridine (Me-14-C)methyl ester residues when \"saponified\" yeast tRNA is incubated with S-adenosyl(Me-14-C)methionine and homologous (but not heterologous) enzymes. (3) When selectively labelled yeast tRNA is hydrolyzed by RNase T-1, both 5-carboxymethyluridine (Me-14-C)methyl ester and its 2-thio-analogue are released as part of large oligonucleotides, each of which contains roughly 10 nucleotide residues. (4) There are at least three, and possibly four (Me-14-C)methyl ester-containing oligonucleotides released by RNase T-1 digestion of selectively labelled \"saponified\" yeast tRNA. A comparison of the chromatographic properties of the different (Me-14-C)oligonucleotides suggests that the same 5-carboxymethyluridine residues are probably targets for both homologous and heterologous enzymes. (5) The properties of the selectively labelled oligonucleotides are consistent with the view that some of them probably are derived from yeast tRNA-3-Glu, tRNA-2-Lys, and tRNA-3-Arg, all of which are known to contain 5-carboxymethyl methyl esters as part of their anticodon sequences.", "contents": "Selective labelling ot the methyl carboxylate substituents found in the anticodon sequences of some species of yeast transfer RNA. (1) By incubation in 0.1 M NaOH for 10 min at room temperature, it is possible to \"saponify\" some of the methyl carboxylate linkages in bulk yeast tRNA. By incubation with S-adenosyl(Me-14-C)methionine and either homologous (yeast) or heterologous (wheat-embryo) enzymes, it is then possible to \"re-esterify\" the \"saponified\" tRNA and thereby effect selective labelling at 5-carboxymethyluridine (Me-14-C)methyl ester residues. (2) There is also selective labelling at 2-thio-5-carboxymethyluridine (Me-14-C)methyl ester residues when \"saponified\" yeast tRNA is incubated with S-adenosyl(Me-14-C)methionine and homologous (but not heterologous) enzymes. (3) When selectively labelled yeast tRNA is hydrolyzed by RNase T-1, both 5-carboxymethyluridine (Me-14-C)methyl ester and its 2-thio-analogue are released as part of large oligonucleotides, each of which contains roughly 10 nucleotide residues. (4) There are at least three, and possibly four (Me-14-C)methyl ester-containing oligonucleotides released by RNase T-1 digestion of selectively labelled \"saponified\" yeast tRNA. A comparison of the chromatographic properties of the different (Me-14-C)oligonucleotides suggests that the same 5-carboxymethyluridine residues are probably targets for both homologous and heterologous enzymes. (5) The properties of the selectively labelled oligonucleotides are consistent with the view that some of them probably are derived from yeast tRNA-3-Glu, tRNA-2-Lys, and tRNA-3-Arg, all of which are known to contain 5-carboxymethyl methyl esters as part of their anticodon sequences."}
{"id": "PMID:1091334", "title": "An improved method for the isolation of surface membranes from cells in monolayer culture.", "content": "A modification of the procedure reported by Scher and Barland (Biochim. Biophys. Acta 255 (1972) 580-588) for the isolation of the surface membranes of cells grown in monolayer culture is described. The modification involves addition of a purification step utilizing the two-phase polymer system described by Brunette and Till (J. Membr. Biol. 5 (1971) 215-224). The modified method permits the isolation of surface membranes from cells not yet at confluence, when they may easily become detached from the substratum.", "contents": "An improved method for the isolation of surface membranes from cells in monolayer culture. A modification of the procedure reported by Scher and Barland (Biochim. Biophys. Acta 255 (1972) 580-588) for the isolation of the surface membranes of cells grown in monolayer culture is described. The modification involves addition of a purification step utilizing the two-phase polymer system described by Brunette and Till (J. Membr. Biol. 5 (1971) 215-224). The modified method permits the isolation of surface membranes from cells not yet at confluence, when they may easily become detached from the substratum."}
{"id": "PMID:1091335", "title": "Degradation of RNA in nuclei from Ehrlich ascites cells.", "content": "Nuclei were prepared from Ehrlich ascites cells in 80% yield by homogenization of the cells in an aqueous solution containing Triton N-101 and washing of the nuclear fraction by centrifugation and resuspension. Compared to the enzyme activities present in cell extracts, approximately 47% exo-RNase I, 15% alkaline RNase II, 9% acid RNase II and 7% acid phosphatase were associated with the nuclear fraction after isolation. Exo-RNase I and alkaline RNase II were rapidly lost from nuclei during incubation at 37 degrees C. The degradation of newly synthesized RNA in nuclei incubated at 37 degrees C was followed by polyacrylamide gel electrophoresis and by characterization of acid-soluble degradation products. The rate of hydrolysis of the nuclear RNA was rapid during the initial stages of incubation and then proceeded at a much reduced rate. Nucleoside 5'-phosphates were the major acid-soluble degradation products, in agreement with the presence of exo-RNase I. Although a considerable amount of alkaline RNase II was associated with the nuclear fraction, extensive endonucleolytic cleavage of the nuclear RNA was not apparent. Compared to the processing of nuclear RNA in whole cells, however, the degradation in isolated nuclei was relatively non-specific.", "contents": "Degradation of RNA in nuclei from Ehrlich ascites cells. Nuclei were prepared from Ehrlich ascites cells in 80% yield by homogenization of the cells in an aqueous solution containing Triton N-101 and washing of the nuclear fraction by centrifugation and resuspension. Compared to the enzyme activities present in cell extracts, approximately 47% exo-RNase I, 15% alkaline RNase II, 9% acid RNase II and 7% acid phosphatase were associated with the nuclear fraction after isolation. Exo-RNase I and alkaline RNase II were rapidly lost from nuclei during incubation at 37 degrees C. The degradation of newly synthesized RNA in nuclei incubated at 37 degrees C was followed by polyacrylamide gel electrophoresis and by characterization of acid-soluble degradation products. The rate of hydrolysis of the nuclear RNA was rapid during the initial stages of incubation and then proceeded at a much reduced rate. Nucleoside 5'-phosphates were the major acid-soluble degradation products, in agreement with the presence of exo-RNase I. Although a considerable amount of alkaline RNase II was associated with the nuclear fraction, extensive endonucleolytic cleavage of the nuclear RNA was not apparent. Compared to the processing of nuclear RNA in whole cells, however, the degradation in isolated nuclei was relatively non-specific."}
{"id": "PMID:1091336", "title": "An experimentally pathogenic Bacillus species. I. Description and characterization of the organism.", "content": "A previously underscribed Bacillus species has been characterized. The organism was isolated from a blood transfusion bottle implicated in a fatal bacteriogenic transfusion reaction and has been examined from a number of different aspects to characterize it as completely as necessary to provide identification. It was placed into group 3 of the genus Bacillus on the basis of its cellular morphology, the morphology of the sporangium, and the location of the spore within the cell. There are only three other species described in this group. Since the organism differed markedly from each of these, it appears to represent a new species.", "contents": "An experimentally pathogenic Bacillus species. I. Description and characterization of the organism. A previously underscribed Bacillus species has been characterized. The organism was isolated from a blood transfusion bottle implicated in a fatal bacteriogenic transfusion reaction and has been examined from a number of different aspects to characterize it as completely as necessary to provide identification. It was placed into group 3 of the genus Bacillus on the basis of its cellular morphology, the morphology of the sporangium, and the location of the spore within the cell. There are only three other species described in this group. Since the organism differed markedly from each of these, it appears to represent a new species."}
{"id": "PMID:1091337", "title": "Fungal fimbriae. III. The effect on flocculation in Saccharomyces.", "content": "Flocculent strains of Saccharomyces cerevisiae and S. carlsbergensis (S. uvarum) produced many short (0.5 mum) hairs (fimbriae) on the outer cell walls. Non-flocculent strains produce few fimbriae. Cells that are flocculent in wort but not in defined medium produce fimbriae only in the former medium. Cells treated with pronase lose both their fimbriae and the ability to flocculate. Cells treated with alpha-amylase retain some fimbriae but lose the ability to flocculate. It is suggested that the fimbriae may be the surface mannan-protein complexes known to be involved in flocculation and also in protein secretion. Haploid cells of both mating types also produced fimbriae, some of which apparently have a terminal knob.", "contents": "Fungal fimbriae. III. The effect on flocculation in Saccharomyces. Flocculent strains of Saccharomyces cerevisiae and S. carlsbergensis (S. uvarum) produced many short (0.5 mum) hairs (fimbriae) on the outer cell walls. Non-flocculent strains produce few fimbriae. Cells that are flocculent in wort but not in defined medium produce fimbriae only in the former medium. Cells treated with pronase lose both their fimbriae and the ability to flocculate. Cells treated with alpha-amylase retain some fimbriae but lose the ability to flocculate. It is suggested that the fimbriae may be the surface mannan-protein complexes known to be involved in flocculation and also in protein secretion. Haploid cells of both mating types also produced fimbriae, some of which apparently have a terminal knob."}
{"id": "PMID:1091339", "title": "Evaluation of polyglycolic aicd sutures in colon anastomoses.", "content": "An experimental study was carried out in dogs to compare the healing characteristics of colon anastomoses constructed with silk and catgut, with those in which Dexon sutures of the same gauge were used. Anastomoses constructed with Dexon sutures showed minimal suture reaction on the serosal surfaces, and excellent healing strength as determined by bursting-pressure studies. Inspection of the mucosal surfaces in these anastomoses revealed flawless, smooth, healing suture lines. The flexibility of tissues at the anastomotic line was found to be good, and friability of tissue minimal.", "contents": "Evaluation of polyglycolic aicd sutures in colon anastomoses. An experimental study was carried out in dogs to compare the healing characteristics of colon anastomoses constructed with silk and catgut, with those in which Dexon sutures of the same gauge were used. Anastomoses constructed with Dexon sutures showed minimal suture reaction on the serosal surfaces, and excellent healing strength as determined by bursting-pressure studies. Inspection of the mucosal surfaces in these anastomoses revealed flawless, smooth, healing suture lines. The flexibility of tissues at the anastomotic line was found to be good, and friability of tissue minimal."}
{"id": "PMID:1091340", "title": "Cystic adventitial disease of the popliteal artery.", "content": "The first case of cystic adventitial disease of the popliteal artery recorded in Canada is reported and discussed in conjunction with a review of the world literature of 45 reported cases. This rare cause of arterial insufficiency occurs usually in young men and should be considered in the differential diagnosis of claudication. Angiographic findings are diagnostic and surgery is usually corrective. The theories of etiology and pathogenesis are discussed and the gross and microscopic pathology is demonstrated.", "contents": "Cystic adventitial disease of the popliteal artery. The first case of cystic adventitial disease of the popliteal artery recorded in Canada is reported and discussed in conjunction with a review of the world literature of 45 reported cases. This rare cause of arterial insufficiency occurs usually in young men and should be considered in the differential diagnosis of claudication. Angiographic findings are diagnostic and surgery is usually corrective. The theories of etiology and pathogenesis are discussed and the gross and microscopic pathology is demonstrated."}
{"id": "PMID:1091342", "title": "Isolation of dermatophytes, Candida species and systemic fungi from dermatologic specimens in Montr\u00e9al, 1963 to 1973.", "content": "Of 10 057 specimens of scrapings from skin, nails and scalp examined for dermatophytes, yeasts, pityriasis versicolor and systemic mycoses between 1963 and 1973, 30.4 percent were positive for fungi. Skin produced the highest proportion (68.6 percent) of positive scrapings, scalp the lowest (4.2 percent). Trichophyton rubrum was the predominant species (23.6 percent); of lesser prevalence were Microsporum canis (9.3 percent), T. mentagrophytes (8.4 percent) and Epidermophyton floccosum (4.8 percent). Double infections were encountered on 102 occasions; T. rubrum and Candida parapsilosis were the most frequent cohabiting species. The introduction in 1966 of periodic acid-Schiff staining for routine examination of scrapings resulted in better diagnostic results, particularly in the case of culturally nonproductive specimens and cases of pityriasis versicolor. Blastomyces dermatitidis and Cryptococcus neoformans were isolated from two patients in the course of routine investigation for dermatophytes.", "contents": "Isolation of dermatophytes, Candida species and systemic fungi from dermatologic specimens in Montr\u00e9al, 1963 to 1973. Of 10 057 specimens of scrapings from skin, nails and scalp examined for dermatophytes, yeasts, pityriasis versicolor and systemic mycoses between 1963 and 1973, 30.4 percent were positive for fungi. Skin produced the highest proportion (68.6 percent) of positive scrapings, scalp the lowest (4.2 percent). Trichophyton rubrum was the predominant species (23.6 percent); of lesser prevalence were Microsporum canis (9.3 percent), T. mentagrophytes (8.4 percent) and Epidermophyton floccosum (4.8 percent). Double infections were encountered on 102 occasions; T. rubrum and Candida parapsilosis were the most frequent cohabiting species. The introduction in 1966 of periodic acid-Schiff staining for routine examination of scrapings resulted in better diagnostic results, particularly in the case of culturally nonproductive specimens and cases of pityriasis versicolor. Blastomyces dermatitidis and Cryptococcus neoformans were isolated from two patients in the course of routine investigation for dermatophytes."}
{"id": "PMID:1091343", "title": "Winter illness and vitamin C: the effect of relatively low doses.", "content": "After their random -llocation to one of three treatment aroups, 622 volunteers received either vitamin C or placebo in a maintenance dose of 500 mg once weekly and a therapeutic dose of 1500 mg daily on the 1st day and 1000 mg on the next 4 days of any illness. Two forms of vitamin C were employed: a sustained-release capsule containing ascorbic acid and a regular tabet containing a mixture of sodium and calcium ascorbate. In the 448 subjects who completed an average of 15 weeks in the study of total of 635 episodes of illness were recroded. Respiratory symptoms were recorded on at least 1 day in 92 per cent of these episodes. There were no consistent or significant differences in the sickness experience of the subjects receiving the sustained-release vitamin capsules compared to those receiving the vitamin tablets, but subjects in both vitamin groups experienced less severe illness than subjects in the placebo group, with approximately 25 per cent fewer days spent indoors because of the illness (P smaller than 0.05). These results are  compatible with the belief that supplementary vitamin C can reduce the burden of winter illness, but the intake need not be as high as has sometimes been claimed.", "contents": "Winter illness and vitamin C: the effect of relatively low doses. After their random -llocation to one of three treatment aroups, 622 volunteers received either vitamin C or placebo in a maintenance dose of 500 mg once weekly and a therapeutic dose of 1500 mg daily on the 1st day and 1000 mg on the next 4 days of any illness. Two forms of vitamin C were employed: a sustained-release capsule containing ascorbic acid and a regular tabet containing a mixture of sodium and calcium ascorbate. In the 448 subjects who completed an average of 15 weeks in the study of total of 635 episodes of illness were recroded. Respiratory symptoms were recorded on at least 1 day in 92 per cent of these episodes. There were no consistent or significant differences in the sickness experience of the subjects receiving the sustained-release vitamin capsules compared to those receiving the vitamin tablets, but subjects in both vitamin groups experienced less severe illness than subjects in the placebo group, with approximately 25 per cent fewer days spent indoors because of the illness (P smaller than 0.05). These results are  compatible with the belief that supplementary vitamin C can reduce the burden of winter illness, but the intake need not be as high as has sometimes been claimed."}
{"id": "PMID:1091344", "title": "Extensive intraglomerular fibrin deposition after renal transplantation: recovery without anticoagulation.", "content": "Acute renal failure developed in a 55-year-old man 6 days after he had received a cadaver renal allograft. This was associated with thrombocytopenia. Extensive intraglomerular fibrin deposition was seen in a renal biopsy specimen. He was treated with corticosteroids, azathioprine, cyclophosphamide and hemodialysis with regional heparinization but not with systemic anticoagulation. This was followed by complete recovery of both renal function and histologic damage despite the fact that he did not receive anticoagulant therapy. This suggests that treatment with anticoagulants may not be necessary for all patients with intraglomerular deposits of fibrin.", "contents": "Extensive intraglomerular fibrin deposition after renal transplantation: recovery without anticoagulation. Acute renal failure developed in a 55-year-old man 6 days after he had received a cadaver renal allograft. This was associated with thrombocytopenia. Extensive intraglomerular fibrin deposition was seen in a renal biopsy specimen. He was treated with corticosteroids, azathioprine, cyclophosphamide and hemodialysis with regional heparinization but not with systemic anticoagulation. This was followed by complete recovery of both renal function and histologic damage despite the fact that he did not receive anticoagulant therapy. This suggests that treatment with anticoagulants may not be necessary for all patients with intraglomerular deposits of fibrin."}
{"id": "PMID:1091348", "title": "Reticulum cell sarcoma of the brain. A review of the literature and a study of 19 cases.", "content": "Reticulum cell sarcoma of the brain is a rare neoplasm. However, patients who have been recipients of transplants have had a much higher than expected incidence of this tumor; reticulum cell sarcomas of the brain may be seen more often in the future. Most of the information available in the literature deals with the pathology and clinical course of the disease. Although several authors have commented on the radioresponsiveness of the tumor, little detail as to the exact treatment methods has been provided. Furthermore, it has been assumed that the prognosis of this tumor, after some form of surgery and postoperative radiotherapy, may be fairly good. This paper presents 19 patients who have had surgery followed by postoperative radiotherapy. Although a few patients have had good relief of symptoms for 3 to 4 years, only 1 patient is alive and free of disease over 5 years. This patient is the sixth reported long-term survival in the literature. Suggestions for the management of this disease are provided. If possible, limited surgery to remove circumscribed tumors should be done, but extensive resection should be avoided. Postoperative irradiation should include the whole brain to at least 4500 rads at 1000 rads per week, with a boost to residual tumor, if present, of 500 to 1000 rads in two to five treatments.", "contents": "Reticulum cell sarcoma of the brain. A review of the literature and a study of 19 cases. Reticulum cell sarcoma of the brain is a rare neoplasm. However, patients who have been recipients of transplants have had a much higher than expected incidence of this tumor; reticulum cell sarcomas of the brain may be seen more often in the future. Most of the information available in the literature deals with the pathology and clinical course of the disease. Although several authors have commented on the radioresponsiveness of the tumor, little detail as to the exact treatment methods has been provided. Furthermore, it has been assumed that the prognosis of this tumor, after some form of surgery and postoperative radiotherapy, may be fairly good. This paper presents 19 patients who have had surgery followed by postoperative radiotherapy. Although a few patients have had good relief of symptoms for 3 to 4 years, only 1 patient is alive and free of disease over 5 years. This patient is the sixth reported long-term survival in the literature. Suggestions for the management of this disease are provided. If possible, limited surgery to remove circumscribed tumors should be done, but extensive resection should be avoided. Postoperative irradiation should include the whole brain to at least 4500 rads at 1000 rads per week, with a boost to residual tumor, if present, of 500 to 1000 rads in two to five treatments."}
{"id": "PMID:1091349", "title": "Antipyretic effect of cycloheximide, and inhibitor of protein synthesis, in patients with Hodgkin's disease or other malignant neoplasms.", "content": "Infusion of cycloheximide i.v., an antibiotic known to inhibit synthesis of protein, at a rate of 0.2 mg/kg/hr, reliably caused lysis of fever in 15 chronically febrile patients with Hodgkin's disease who did not have detectable bacterial, fungal, or viral infection. Antipyretic effects were also seen in some patients with reticulum cell sarcoma, lymphosarcoma, acute leukemia, histiocytic medullary reticulosis, plasma cell myeloma, carcinoma of the lung, and carcinoma of the cervix. The drug failed to produce defervescence in four patients with normal granulocyte reserves, who were febrile due to bacterial infection. When infused at a rate of 0.2 mg/kg/hr, the drug apparently caused an acute alteration of protein metabolism in man in that plasma amino acid nitrogen rose acutely while plasma levels of muramidase and ribonuclease fell during the period of the infusion. The data suggest that continuing synthesis of protein may be involved in nonbacterial fever of neoplastic disease. Mammalian granulocytes and monocytes are known to elaborate a pyrogenic protein following appropriate stimulation; it is suggested that in some types of neoplastic disease, particularly Hodgkin's disease, tumor cells may produce and release a pyrogenic protein and that drug-induced inhibition of its synthesis is responsible for the observed lysis of fever.", "contents": "Antipyretic effect of cycloheximide, and inhibitor of protein synthesis, in patients with Hodgkin's disease or other malignant neoplasms. Infusion of cycloheximide i.v., an antibiotic known to inhibit synthesis of protein, at a rate of 0.2 mg/kg/hr, reliably caused lysis of fever in 15 chronically febrile patients with Hodgkin's disease who did not have detectable bacterial, fungal, or viral infection. Antipyretic effects were also seen in some patients with reticulum cell sarcoma, lymphosarcoma, acute leukemia, histiocytic medullary reticulosis, plasma cell myeloma, carcinoma of the lung, and carcinoma of the cervix. The drug failed to produce defervescence in four patients with normal granulocyte reserves, who were febrile due to bacterial infection. When infused at a rate of 0.2 mg/kg/hr, the drug apparently caused an acute alteration of protein metabolism in man in that plasma amino acid nitrogen rose acutely while plasma levels of muramidase and ribonuclease fell during the period of the infusion. The data suggest that continuing synthesis of protein may be involved in nonbacterial fever of neoplastic disease. Mammalian granulocytes and monocytes are known to elaborate a pyrogenic protein following appropriate stimulation; it is suggested that in some types of neoplastic disease, particularly Hodgkin's disease, tumor cells may produce and release a pyrogenic protein and that drug-induced inhibition of its synthesis is responsible for the observed lysis of fever."}
{"id": "PMID:1091350", "title": "Effect of Acinetobacter glutaminase-asparaginase treatment on free amino acids in mouse tissues.", "content": "Acinetobacter glutaminase-asparaginase (AGA) and Escherichia coli asparaginase were compared for their effects on plasma and tissue levels of amino acids, ammonia, and glutamyl transferase activity in the mouse. Free asparagine was depleted similarly in plasma and tissues by both enzymes. AGA treatment produced partial depletion of glutamine concentrations in muscle, spleen, small intestine, and liver. Brain and kidney glutamine concentrations actually rose with treatment. Despite over 100-fold increase in plasma glutamate, only the kidney showed a substantial increase in free glutamate levels during AGA treatment. Glutamine biosynthesis measured by glutamyl transferase activity showed an appreciable increase only in the kidney. Ammonia levels in tissues and plasma rose 1.3- to 4.3-fold. In general, E. coli asparaginase treatment had much less effect on these measurements than did AGA. The changes in these levels are discussed in relation to sites of possible toxicity and antitumor effects.", "contents": "Effect of Acinetobacter glutaminase-asparaginase treatment on free amino acids in mouse tissues. Acinetobacter glutaminase-asparaginase (AGA) and Escherichia coli asparaginase were compared for their effects on plasma and tissue levels of amino acids, ammonia, and glutamyl transferase activity in the mouse. Free asparagine was depleted similarly in plasma and tissues by both enzymes. AGA treatment produced partial depletion of glutamine concentrations in muscle, spleen, small intestine, and liver. Brain and kidney glutamine concentrations actually rose with treatment. Despite over 100-fold increase in plasma glutamate, only the kidney showed a substantial increase in free glutamate levels during AGA treatment. Glutamine biosynthesis measured by glutamyl transferase activity showed an appreciable increase only in the kidney. Ammonia levels in tissues and plasma rose 1.3- to 4.3-fold. In general, E. coli asparaginase treatment had much less effect on these measurements than did AGA. The changes in these levels are discussed in relation to sites of possible toxicity and antitumor effects."}
{"id": "PMID:1091351", "title": "Electrophysiological effects of the antiarrhythmic agent QX-572 in the human heart with special reference to rate-induced changes in effective refractory periods.", "content": "The antiarrhythmic agent, N,N-bis(phenylcarbamoylmethyl)dimethylammonium chloride (QX-572) 8 mg/kg body weight produced a decreased conduction time through the atrioventricular node but did not influence intra-atrial or intraventricular conduction. Effective refractory periods of the atrium, the atrioventricular conduction system, and the ventricle were shortened by the drug. The observed effects were present at the end of drug infusion but had disapnerbd 30 min later. The probable explanation is an increased sympathetic tone induced by QX-572.", "contents": "Electrophysiological effects of the antiarrhythmic agent QX-572 in the human heart with special reference to rate-induced changes in effective refractory periods. The antiarrhythmic agent, N,N-bis(phenylcarbamoylmethyl)dimethylammonium chloride (QX-572) 8 mg/kg body weight produced a decreased conduction time through the atrioventricular node but did not influence intra-atrial or intraventricular conduction. Effective refractory periods of the atrium, the atrioventricular conduction system, and the ventricle were shortened by the drug. The observed effects were present at the end of drug infusion but had disapnerbd 30 min later. The probable explanation is an increased sympathetic tone induced by QX-572."}
{"id": "PMID:1091353", "title": "Immunohistochemical localization of human pancreatic polypeptide (HPP) to a population of islet cells.", "content": "HPP is the human counterpart of APP--a polypeptide that has been isolated from avian pancreas and shown to exert pronounced biological effects. The availability of anti-HPP serum enabled us to use immunohistochemical procedures for the localization of HPP in human pancreas. A small population of pancreatic islet cells showed strong immunofluorescence after staining with anti-HPP serum. The HPP cells were mainly localized at the periphery of the islets. Sometimes they were also seen scattered in the exocrine parenchyma as well as within the epithelium of small to medium-sized ducts. While rare in the pancreas of adult man, HPP cells were abundant in certain parts of the pancreas of foetuses (18-20 weeks gestational age). Their staining properties showed them to be distinct from the A, Band D cells of the pancreatic islets.", "contents": "Immunohistochemical localization of human pancreatic polypeptide (HPP) to a population of islet cells. HPP is the human counterpart of APP--a polypeptide that has been isolated from avian pancreas and shown to exert pronounced biological effects. The availability of anti-HPP serum enabled us to use immunohistochemical procedures for the localization of HPP in human pancreas. A small population of pancreatic islet cells showed strong immunofluorescence after staining with anti-HPP serum. The HPP cells were mainly localized at the periphery of the islets. Sometimes they were also seen scattered in the exocrine parenchyma as well as within the epithelium of small to medium-sized ducts. While rare in the pancreas of adult man, HPP cells were abundant in certain parts of the pancreas of foetuses (18-20 weeks gestational age). Their staining properties showed them to be distinct from the A, Band D cells of the pancreatic islets."}
{"id": "PMID:1091352", "title": "Fluorescence histochemical and elec-ron microscopical observations on sympathetic ganglia of the chick embryo cultured with and without hydrocortisone.", "content": "Lumbar sympathetic ganglia of 12-day-old chick embryos were cultured in organ  cultures for 14 days with 1, 10 or 100 mg/l of hydrocortisone or without it. Catecholamines were demonstrated by the formaldehyde-induced fluorescence method. For electron microscopy, the cultures were fixed with glutarialdehyde and osmium tetroxide. Two types of cells with catecholamine fluoresecence were observed in the control cultures: (1) weakly fluorescent sympathetic neurons and sympathicoblasts with long nerve fibres, which were the most common cell type in the explant, and (2) brightly fluorescent cells with or without fluorescent processes, which were less common and were scattered in the explant. Hydrocortisone caused a great increase in the number of the brightly fluorescent cells. With 10 mg/l of hydrocortisone the increase was about ten-fold as compared with the control cultures. There was no change in the morphology of the cells, nor could any change be observed in the fluorescence intensity by eye. Electron microscopically the mature neurons were the most common cell type on the surface of the culture, while more immature sympathicoblasts were seen in the deeper layers. Cells were also found which contained large numbers of catecholamine-strong granular vesicles 105-275 nm in diameter. These cells were infrequent. They had round vesicular nuclei and resembled also in other respects sympathicoblasts or young nerve cells. One such cell was found in mitotic division by electron microscopy. Hydrocortisone caused a marked increase in the number of these granule-containing cells and their processes. Cells which could have been classified as the small intensely fluorescent cells of the mammalian ganglion type or their electron microscopic equivalent, the granule-containing cells were found neither in the control cultures nor in the hydrocortisone-containing cultures. It is concluded that most brightly fluorescent cells in cultured sympathetic ganglia of the chick are nerve cells or sympathicoblasts rich in amine-storing granular vesicles.", "contents": "Fluorescence histochemical and elec-ron microscopical observations on sympathetic ganglia of the chick embryo cultured with and without hydrocortisone. Lumbar sympathetic ganglia of 12-day-old chick embryos were cultured in organ  cultures for 14 days with 1, 10 or 100 mg/l of hydrocortisone or without it. Catecholamines were demonstrated by the formaldehyde-induced fluorescence method. For electron microscopy, the cultures were fixed with glutarialdehyde and osmium tetroxide. Two types of cells with catecholamine fluoresecence were observed in the control cultures: (1) weakly fluorescent sympathetic neurons and sympathicoblasts with long nerve fibres, which were the most common cell type in the explant, and (2) brightly fluorescent cells with or without fluorescent processes, which were less common and were scattered in the explant. Hydrocortisone caused a great increase in the number of the brightly fluorescent cells. With 10 mg/l of hydrocortisone the increase was about ten-fold as compared with the control cultures. There was no change in the morphology of the cells, nor could any change be observed in the fluorescence intensity by eye. Electron microscopically the mature neurons were the most common cell type on the surface of the culture, while more immature sympathicoblasts were seen in the deeper layers. Cells were also found which contained large numbers of catecholamine-strong granular vesicles 105-275 nm in diameter. These cells were infrequent. They had round vesicular nuclei and resembled also in other respects sympathicoblasts or young nerve cells. One such cell was found in mitotic division by electron microscopy. Hydrocortisone caused a marked increase in the number of these granule-containing cells and their processes. Cells which could have been classified as the small intensely fluorescent cells of the mammalian ganglion type or their electron microscopic equivalent, the granule-containing cells were found neither in the control cultures nor in the hydrocortisone-containing cultures. It is concluded that most brightly fluorescent cells in cultured sympathetic ganglia of the chick are nerve cells or sympathicoblasts rich in amine-storing granular vesicles."}
{"id": "PMID:1091354", "title": "Electron microscope study on the occurrence of electron-dense deposits at the cell membrane of chicken erythrocytes.", "content": "Electron-dense deposits were found along the inner side of the cell membrane of chicken erythrocytes when fixed in a CaCl2-containing glutaraldehyde solution. Osteoblasts and osteoclasts were studied in an attempt to determine whether the deposits are related to calcium metabolism. No electron-dense deposits adjacent to the cell membrane were found in either these cells or in rat erythrocytes. Therefore, it is assumed that the deposits are not calcium-binding protein necessary for the cellular calcium uptake.", "contents": "Electron microscope study on the occurrence of electron-dense deposits at the cell membrane of chicken erythrocytes. Electron-dense deposits were found along the inner side of the cell membrane of chicken erythrocytes when fixed in a CaCl2-containing glutaraldehyde solution. Osteoblasts and osteoclasts were studied in an attempt to determine whether the deposits are related to calcium metabolism. No electron-dense deposits adjacent to the cell membrane were found in either these cells or in rat erythrocytes. Therefore, it is assumed that the deposits are not calcium-binding protein necessary for the cellular calcium uptake."}
{"id": "PMID:1091355", "title": "Electron microscopie and enzyme cytochemical studies on the guinia pig metaphysis with special reference to the lysosomal system of different cell types.", "content": "A transmission electron microscopic study of demineralized, methaphyseal bone of the young guinea pig is presented. Special attention is paid to the lysosomal system of the different cell types. Visualization of acid phosphatase and aryl sulfatase activity was used to identify tissue components as belonging thereto. The distribution of alkaline phosphatase activity, a plasma membrane marker, was also examined. Osteoblasts were distinguished by a marked development of the granular endoplasmic reticulum and the Golgi complex. Perivascular cells type A, morphologically resembled the osteoblasts, and are believed to represent an early stage in the specialization of the latter. A few lysosomes were normally found in the osteoblasts; they were less common in the type A cells. In contrasts to their regular occurrence in guinea pig epiphyseal cartilage, dense bodies of lysosomal nature (\"type I vesicles\") were only rarely seen in the bone matrix. Structures analogous to the type II vesicles in cartilage were, however, normally present. Their membrane showed activity of alkaline phosphatase. Possible functions of lysosomes and matrix vesicles in osteogenesis are discussed. Perivascular cells type B and chondroclasts both contained a prominent Golgi complex and large numbers of free ribosomes, mitochondria and lysosomes. In the type B cells, inclusion material of varying appearance often occurred in the lysosomes and in endocytic vesicles. The chondroclasts sometimes presented a ruffled border, with associated vacuoles and lysosomes in the subjacent cytoplasm. It is suggested that both cell types participate in the resorption of the epiphyseal cartilage. Chondroclasts presumably arise by fusion of type B cells and/or monocytic precursors from the peripheral blood.", "contents": "Electron microscopie and enzyme cytochemical studies on the guinia pig metaphysis with special reference to the lysosomal system of different cell types. A transmission electron microscopic study of demineralized, methaphyseal bone of the young guinea pig is presented. Special attention is paid to the lysosomal system of the different cell types. Visualization of acid phosphatase and aryl sulfatase activity was used to identify tissue components as belonging thereto. The distribution of alkaline phosphatase activity, a plasma membrane marker, was also examined. Osteoblasts were distinguished by a marked development of the granular endoplasmic reticulum and the Golgi complex. Perivascular cells type A, morphologically resembled the osteoblasts, and are believed to represent an early stage in the specialization of the latter. A few lysosomes were normally found in the osteoblasts; they were less common in the type A cells. In contrasts to their regular occurrence in guinea pig epiphyseal cartilage, dense bodies of lysosomal nature (\"type I vesicles\") were only rarely seen in the bone matrix. Structures analogous to the type II vesicles in cartilage were, however, normally present. Their membrane showed activity of alkaline phosphatase. Possible functions of lysosomes and matrix vesicles in osteogenesis are discussed. Perivascular cells type B and chondroclasts both contained a prominent Golgi complex and large numbers of free ribosomes, mitochondria and lysosomes. In the type B cells, inclusion material of varying appearance often occurred in the lysosomes and in endocytic vesicles. The chondroclasts sometimes presented a ruffled border, with associated vacuoles and lysosomes in the subjacent cytoplasm. It is suggested that both cell types participate in the resorption of the epiphyseal cartilage. Chondroclasts presumably arise by fusion of type B cells and/or monocytic precursors from the peripheral blood."}
{"id": "PMID:1091356", "title": "The fate of injected human IgG in the mouse liver: Uptake, immunological inactivation, and lysosomal reactions.", "content": "1. Human IgG is stored in the form of remarkable droplets in the cytoplasm of mouse hepatocytes following intravenous injection. 2. The earliest protein uptake occurs within 1 minute after injection and droplets can be found up to 32 hours thereafter; 64 hours p.i. the foreign protein is no longer visible in hepatocytes. 3. Electron microscopy reveals that the uptake occurs by a process of \"macropinocytosis\". The resulting protein droplets fuse with primary lysosomes and are transformed into phagolysosomes. 4. At the same time as the phagolysosomes are formed the IgG-droplets loose their immunological activity--as early as 2 hours after injection.", "contents": "The fate of injected human IgG in the mouse liver: Uptake, immunological inactivation, and lysosomal reactions. 1. Human IgG is stored in the form of remarkable droplets in the cytoplasm of mouse hepatocytes following intravenous injection. 2. The earliest protein uptake occurs within 1 minute after injection and droplets can be found up to 32 hours thereafter; 64 hours p.i. the foreign protein is no longer visible in hepatocytes. 3. Electron microscopy reveals that the uptake occurs by a process of \"macropinocytosis\". The resulting protein droplets fuse with primary lysosomes and are transformed into phagolysosomes. 4. At the same time as the phagolysosomes are formed the IgG-droplets loose their immunological activity--as early as 2 hours after injection."}
{"id": "PMID:1091357", "title": "Subcellular localization of calcium in the mouse hypophysis. I. Calcium distribution in the adeno- and neurohypophysis under normal conditions.", "content": "Application of the K-pyroantimonate technique combined with glutaraldehydeosmium fixation results in a reproducible intracellular distribution of mineral precipitates in the mouse hypophysis. Control experiments--with chelators and electron probe microanalysis--reveal that these precipitates consist mainly of calcium. Regularly present in the mitochondria, Ca also seems to be stored in the Golgi apparatus of the glandular cells and in the axoplasmic reticulum and the \"synaptic\" vesicles of the neurosecretory fibres. These structures thus appear able to control intracytoplasmic calcium movements. These observations agree with physiological data showing the existence of an intracellular Ca pool that can be mobilized by specific stimulation. The presence of diffuse precipitates in the pituicytes, together with the existence of gap junctions between them, suggest that these cells regulate the ionic environment of the neurosecretory nerve fibres; in this way, they too might participate in neurohypophysial hormonal release.", "contents": "Subcellular localization of calcium in the mouse hypophysis. I. Calcium distribution in the adeno- and neurohypophysis under normal conditions. Application of the K-pyroantimonate technique combined with glutaraldehydeosmium fixation results in a reproducible intracellular distribution of mineral precipitates in the mouse hypophysis. Control experiments--with chelators and electron probe microanalysis--reveal that these precipitates consist mainly of calcium. Regularly present in the mitochondria, Ca also seems to be stored in the Golgi apparatus of the glandular cells and in the axoplasmic reticulum and the \"synaptic\" vesicles of the neurosecretory fibres. These structures thus appear able to control intracytoplasmic calcium movements. These observations agree with physiological data showing the existence of an intracellular Ca pool that can be mobilized by specific stimulation. The presence of diffuse precipitates in the pituicytes, together with the existence of gap junctions between them, suggest that these cells regulate the ionic environment of the neurosecretory nerve fibres; in this way, they too might participate in neurohypophysial hormonal release."}
{"id": "PMID:1091361", "title": "Replicating circular DNA molecules in yeast.", "content": "The yeast Saccharomyces cerevisiae contains a class of small circular DNA molecules, approximately 2 mum in contour length (Sinclair et al., 1967). In this report, it is shown that these molecules replicate as double-branched circles, similar to those observed during replication of the bacteriophage lambda and Escherichia coli chromosomes. A normal rate of replication of these DNA circles requires the function of a nuclear gene, cdc 8.", "contents": "Replicating circular DNA molecules in yeast. The yeast Saccharomyces cerevisiae contains a class of small circular DNA molecules, approximately 2 mum in contour length (Sinclair et al., 1967). In this report, it is shown that these molecules replicate as double-branched circles, similar to those observed during replication of the bacteriophage lambda and Escherichia coli chromosomes. A normal rate of replication of these DNA circles requires the function of a nuclear gene, cdc 8."}
{"id": "PMID:1091362", "title": "Properties of membrane-associated folded chromosomes of E. coli related to initiation and termination of DNA replication.", "content": "Analysis of folded chromosomes prepared from amino acid-starved E. coli cells of from a dnaC initiation mutant indicates that a unique structure is associated with completion or near completion of rounds of chromosome replication in E. coli. Chromosomes remain associated with portions of the bacterial cell envelope throughout the DNA replication cycle, but become more rapidly sedimenting as replication proceeds in the absence of reinitiation. Before reinitiation of chromosome replication occurs after restoring required amino acids to amino acid-starved cells or after lowering the temperature in a thermosensitive dnaC mutant, sedimentation velocities of the membrane-associated folded chromosomes decrease substantially. The decrease in sedimentation velocity does not depend on renewed DNA synthesis, but does require the activity of at least the dnaC gene product.", "contents": "Properties of membrane-associated folded chromosomes of E. coli related to initiation and termination of DNA replication. Analysis of folded chromosomes prepared from amino acid-starved E. coli cells of from a dnaC initiation mutant indicates that a unique structure is associated with completion or near completion of rounds of chromosome replication in E. coli. Chromosomes remain associated with portions of the bacterial cell envelope throughout the DNA replication cycle, but become more rapidly sedimenting as replication proceeds in the absence of reinitiation. Before reinitiation of chromosome replication occurs after restoring required amino acids to amino acid-starved cells or after lowering the temperature in a thermosensitive dnaC mutant, sedimentation velocities of the membrane-associated folded chromosomes decrease substantially. The decrease in sedimentation velocity does not depend on renewed DNA synthesis, but does require the activity of at least the dnaC gene product."}
{"id": "PMID:1091368", "title": "The effects of unsubstituted polycyclic aromatic hydrocarbons on the growth of Escherichia coli.", "content": "The effect on growth of E. coli of a series of 3-, 4-, and 5-ring unsubstituted polycyclic aromatic hydrocarbons (PAH) is determined at concentrations of 10-5M, 10-6M, and 10-7M. The angular hydrocarbon configurations of 1,2-benzanthracene, 1,2,5,6-dibenzanthracene (DIBA), and 3,4-benzpyrene (BP) promote growth; tetracene and pyrene have little or no effect on growth while at most concentrations anthracene, phenanthrene chrysene, 1,2,3,4-dibenzanthracene, and pentacene inhibit bacterial growth. No universal pattern of concentration effect is manifest, but based on the results an angular acene configuration is a necessary condition for growth stimulation.", "contents": "The effects of unsubstituted polycyclic aromatic hydrocarbons on the growth of Escherichia coli. The effect on growth of E. coli of a series of 3-, 4-, and 5-ring unsubstituted polycyclic aromatic hydrocarbons (PAH) is determined at concentrations of 10-5M, 10-6M, and 10-7M. The angular hydrocarbon configurations of 1,2-benzanthracene, 1,2,5,6-dibenzanthracene (DIBA), and 3,4-benzpyrene (BP) promote growth; tetracene and pyrene have little or no effect on growth while at most concentrations anthracene, phenanthrene chrysene, 1,2,3,4-dibenzanthracene, and pentacene inhibit bacterial growth. No universal pattern of concentration effect is manifest, but based on the results an angular acene configuration is a necessary condition for growth stimulation."}
{"id": "PMID:1091369", "title": "Neutral lipids in the cells and cell envelope fractions of aerobic baker's yeast and anaerobic brewer's yeast.", "content": "The neutral lipids from whole cells and cell envelopes of aerobic Saccharomyces cerevisiae and anaerobic Sacch. carlsbergensis and the cell walls isolated from the cell envelopes were analysed. The effect of anaerobiosis was particularly clear on the neutral lipid composition of the plasma membrane. Compared to the anaerobic membrane, the aerobic membrane contained more C16:1, C18:1 and other unsaturated fatty acids, more total sterol, more than ten times as much ergosterol and less than one tenth as much squalene, reflecting differences between the aerobic and anaerobic whole cellmthe main sterol in the aerobic membrane ergosterol, was mainly in the free form, whereas zymosterol, 24(28)-dehydroergosterol, epi- or fecosterol and lanosterol were predominantly esterified. In contrast, the anerobic membrane contained small amounts of biosynthetic sterol precursors of ergosterol (mainly esterified), and was clearly richer in saturated fatty acids having a greater variation in chain length and in 18:2 acid. Both plasma membranes contained a considerable amount of triacyglycerols, while the amount of lower acylglycerols was clearly higher in the anaerobic plasma membrane. The lipid composition of both cell walls were relatively similar, consisting mainly of triacylglycerols and lower acylglycerols.", "contents": "Neutral lipids in the cells and cell envelope fractions of aerobic baker's yeast and anaerobic brewer's yeast. The neutral lipids from whole cells and cell envelopes of aerobic Saccharomyces cerevisiae and anaerobic Sacch. carlsbergensis and the cell walls isolated from the cell envelopes were analysed. The effect of anaerobiosis was particularly clear on the neutral lipid composition of the plasma membrane. Compared to the anaerobic membrane, the aerobic membrane contained more C16:1, C18:1 and other unsaturated fatty acids, more total sterol, more than ten times as much ergosterol and less than one tenth as much squalene, reflecting differences between the aerobic and anaerobic whole cellmthe main sterol in the aerobic membrane ergosterol, was mainly in the free form, whereas zymosterol, 24(28)-dehydroergosterol, epi- or fecosterol and lanosterol were predominantly esterified. In contrast, the anerobic membrane contained small amounts of biosynthetic sterol precursors of ergosterol (mainly esterified), and was clearly richer in saturated fatty acids having a greater variation in chain length and in 18:2 acid. Both plasma membranes contained a considerable amount of triacyglycerols, while the amount of lower acylglycerols was clearly higher in the anaerobic plasma membrane. The lipid composition of both cell walls were relatively similar, consisting mainly of triacylglycerols and lower acylglycerols."}
{"id": "PMID:1091370", "title": "A new method of measuring propagation coefficients and characteristic impedance in blood vessels.", "content": "True propagation coefficients of pulse wave harmonics in an artery can be determined in vivo by measuring pulsatile blood pressure and flow at each of two points along the length of the vessel. These coefficients, which are complex numbers that describe the attenuation and the phase shift imposed on a traveling wave, are independent of the reflected waves in the circulation and thus provide information about the viscoelastic state and other properties of an artery. The equations involved are implicit in standard transmission-line theory, but they have not previously been applied in this particular way to blood vessels. The femoral artery, exposed in situ, was studied in 11 anesthetized dogs. At 1.5 Hz, true attenuation constants averaged 0.0151 nepers/cm, and true phase constants averaged 0.0155 radians/cm. As frequency increased, the apparent phase velocity of flow, in contrast, was relatively low at the first harmonic and rose as frequency increased. True phase velocities lay between the apparent pressure and flow values. Characteristic impedance at 1.5 Hz had an average modulus of 1.76 times 10-4 dyne sec/cm5 and a phase of minus 0.31 radians. The modulus diminished as frequency increased, and the phase became less negative. These results show that true phase constants and characteristic impedances determined by this method are consistent with data reported by others and provide information not previously available about flow wave propagation.", "contents": "A new method of measuring propagation coefficients and characteristic impedance in blood vessels. True propagation coefficients of pulse wave harmonics in an artery can be determined in vivo by measuring pulsatile blood pressure and flow at each of two points along the length of the vessel. These coefficients, which are complex numbers that describe the attenuation and the phase shift imposed on a traveling wave, are independent of the reflected waves in the circulation and thus provide information about the viscoelastic state and other properties of an artery. The equations involved are implicit in standard transmission-line theory, but they have not previously been applied in this particular way to blood vessels. The femoral artery, exposed in situ, was studied in 11 anesthetized dogs. At 1.5 Hz, true attenuation constants averaged 0.0151 nepers/cm, and true phase constants averaged 0.0155 radians/cm. As frequency increased, the apparent phase velocity of flow, in contrast, was relatively low at the first harmonic and rose as frequency increased. True phase velocities lay between the apparent pressure and flow values. Characteristic impedance at 1.5 Hz had an average modulus of 1.76 times 10-4 dyne sec/cm5 and a phase of minus 0.31 radians. The modulus diminished as frequency increased, and the phase became less negative. These results show that true phase constants and characteristic impedances determined by this method are consistent with data reported by others and provide information not previously available about flow wave propagation."}
{"id": "PMID:1091378", "title": "Quantitative studies of lipoprotein-X in familial lecithin: cholesterol acyltransferase deficiency and during cholesterol esterification.", "content": "In eight patients with familial lecithin: cholesterol acyltransferase (LCAT) deficiency the plasma concentration of the abnormal lipoprotein LP-X ranged from 43 mg/100 ml to 251 mg/100 ml with a mean of 127 mg/100 ml. This is above the mean level of LP-X found in a group of patients with intrahepatic cholestasis (49 mg/100 ml) and below the mean level found in patients with extrahepatic cholestasis (341 mg/100 ml). Following blood transfusions, an increase in LCAT activity and a decrease in the plasma concentration of LP-X were observed. This decrease was not a simple dilution phenomenon. Quantitation of LP-X during long-term incubation of a whole plasma system demonstrated that LP-X was a source of free cholesterol (FC) in the LCAT reaction. More free cholesterol was derived from LP-X during cholesterol esterification than corresponding to the percentage of the plasma free cholesterol found in the LP-X fraction. The present investigation did not answer the question whether LP-X only was an easily accessible source of free cholesterol for cholesterol esterification or whether LP-X acted directly as a substrate for the LCAT reaction.", "contents": "Quantitative studies of lipoprotein-X in familial lecithin: cholesterol acyltransferase deficiency and during cholesterol esterification. In eight patients with familial lecithin: cholesterol acyltransferase (LCAT) deficiency the plasma concentration of the abnormal lipoprotein LP-X ranged from 43 mg/100 ml to 251 mg/100 ml with a mean of 127 mg/100 ml. This is above the mean level of LP-X found in a group of patients with intrahepatic cholestasis (49 mg/100 ml) and below the mean level found in patients with extrahepatic cholestasis (341 mg/100 ml). Following blood transfusions, an increase in LCAT activity and a decrease in the plasma concentration of LP-X were observed. This decrease was not a simple dilution phenomenon. Quantitation of LP-X during long-term incubation of a whole plasma system demonstrated that LP-X was a source of free cholesterol (FC) in the LCAT reaction. More free cholesterol was derived from LP-X during cholesterol esterification than corresponding to the percentage of the plasma free cholesterol found in the LP-X fraction. The present investigation did not answer the question whether LP-X only was an easily accessible source of free cholesterol for cholesterol esterification or whether LP-X acted directly as a substrate for the LCAT reaction."}
{"id": "PMID:1091381", "title": "Neonatal and childhood gonococcal infections.", "content": "Maternal, fetal, and neonatal morbidity and mortality can be significantly decreased by selective screening of prenatal patients for gonorrhea, early treatment, and follow-up cultures after treatment for gonorrhea. Ophthalmia is the most significant neonatal gonococcal infection. Administration of silver nitrate at delivery is the best prophylaxis against gonococcal ophthalmia. Treatment of gonococcal ophthalmia, however, requires parenteral penicillin, conjunctival antimicrobial therapy, and hospitalization. Treatment of the gonococci-infected mother is also indicated. Childhood gonorrhea is most commonly manifested as vulvovaginitis in girls or urethritis in boys, usually without associated disseminated gonococcal infection. Transmission can occur by indirect contact with an infected parent or involuntary or voluntary sexual activity: in children over age 10 years transmission of gonococci most commonly involves voluntary sexual activity. Emphasis should be placed on early treatment of the infected child, follow-up cultures, and contact tracing. Diagnosis of gonococcal infection requires adequate specimens, gram stains, and cultures for N. gonorrhoeae. In disseminated neonatal gonococcal disease, gram strains and cultures of the conjunctiva, oropharynx, orogastric aspirates, anogenital area, umbilicus, and external ear canal frequently aid in the diagnosis. Frequently, a gram strain of the urethral discharge in boys is sufficient for diagnosis of gonorrhea; in girls cultures are necessary. Since the incidence of gonorrhea in children has increased, the importance of epidemiologic analysis and follow-up after treatment can not be overemphasized.", "contents": "Neonatal and childhood gonococcal infections. Maternal, fetal, and neonatal morbidity and mortality can be significantly decreased by selective screening of prenatal patients for gonorrhea, early treatment, and follow-up cultures after treatment for gonorrhea. Ophthalmia is the most significant neonatal gonococcal infection. Administration of silver nitrate at delivery is the best prophylaxis against gonococcal ophthalmia. Treatment of gonococcal ophthalmia, however, requires parenteral penicillin, conjunctival antimicrobial therapy, and hospitalization. Treatment of the gonococci-infected mother is also indicated. Childhood gonorrhea is most commonly manifested as vulvovaginitis in girls or urethritis in boys, usually without associated disseminated gonococcal infection. Transmission can occur by indirect contact with an infected parent or involuntary or voluntary sexual activity: in children over age 10 years transmission of gonococci most commonly involves voluntary sexual activity. Emphasis should be placed on early treatment of the infected child, follow-up cultures, and contact tracing. Diagnosis of gonococcal infection requires adequate specimens, gram stains, and cultures for N. gonorrhoeae. In disseminated neonatal gonococcal disease, gram strains and cultures of the conjunctiva, oropharynx, orogastric aspirates, anogenital area, umbilicus, and external ear canal frequently aid in the diagnosis. Frequently, a gram strain of the urethral discharge in boys is sufficient for diagnosis of gonorrhea; in girls cultures are necessary. Since the incidence of gonorrhea in children has increased, the importance of epidemiologic analysis and follow-up after treatment can not be overemphasized."}
{"id": "PMID:1091390", "title": "Meperidine kinetics in man. Intravenous injection in surgical patients and volunteers.", "content": "The plasma concentration-time profiles of meperidine following intravenous injection in surgical patients and volunteers were investigated by reference to a classical two-compartment open model. Physiologic characteristics of the subject and variables associated with the surgery and anesthesia were screened as determinants of the kinetic patterns observed. When meperidine administration preceded induction of anesthesia, induction was consistently followed by an increase in venous plasma concentrations that prevented classical kinetic analysis. To facilitate calculations in subsequent studies in patients, meperidine injections were made following induction of anesthesia. Type of anesthesia or premedication, patients' sex, or cigarette smoking did not appear to be important factors in this evaluation. Increasing alcohol consumption was associated with increasing volumes of distribution. Increasing age was associated with increasing fraction of drug unbound in plasma. These factors may relate directly to clinical observations that heavy alcohol consumers tend to be more refractory to central nervous system (CNS) depressants and that elderly patients are more susceptible to respiratory depression from narcotics.", "contents": "Meperidine kinetics in man. Intravenous injection in surgical patients and volunteers. The plasma concentration-time profiles of meperidine following intravenous injection in surgical patients and volunteers were investigated by reference to a classical two-compartment open model. Physiologic characteristics of the subject and variables associated with the surgery and anesthesia were screened as determinants of the kinetic patterns observed. When meperidine administration preceded induction of anesthesia, induction was consistently followed by an increase in venous plasma concentrations that prevented classical kinetic analysis. To facilitate calculations in subsequent studies in patients, meperidine injections were made following induction of anesthesia. Type of anesthesia or premedication, patients' sex, or cigarette smoking did not appear to be important factors in this evaluation. Increasing alcohol consumption was associated with increasing volumes of distribution. Increasing age was associated with increasing fraction of drug unbound in plasma. These factors may relate directly to clinical observations that heavy alcohol consumers tend to be more refractory to central nervous system (CNS) depressants and that elderly patients are more susceptible to respiratory depression from narcotics."}
{"id": "PMID:1091391", "title": "Long-term treatment of hypercholesterolemia with colestipol hydrochloride.", "content": "Colestipol hydroxhloride (15 gm/day) (an anion exchange resin that binds bile acids) of placebo was administered to 92 patients with hypercholesterolemia who were followed for periods up to 36 months. There was a prompt (1 month), significant (p smaller than 0.05 minus 0.001), and sustained (36 months) lowering of serum cholesterol in the colestipol HCl-treated group, but no significant change in the placebo group. Serum triglycerides increased in both treatment groups in parallel; the reason was not apparent. Side effects were equally distributed between colestipol HCl and placebo and were primarily gastrointestinal (upper abdominal distress, constipation). Colestipol HCl appears to be a safe and effective treatment for hypercholesterolemia; tolerance does not seem to develop.", "contents": "Long-term treatment of hypercholesterolemia with colestipol hydrochloride. Colestipol hydroxhloride (15 gm/day) (an anion exchange resin that binds bile acids) of placebo was administered to 92 patients with hypercholesterolemia who were followed for periods up to 36 months. There was a prompt (1 month), significant (p smaller than 0.05 minus 0.001), and sustained (36 months) lowering of serum cholesterol in the colestipol HCl-treated group, but no significant change in the placebo group. Serum triglycerides increased in both treatment groups in parallel; the reason was not apparent. Side effects were equally distributed between colestipol HCl and placebo and were primarily gastrointestinal (upper abdominal distress, constipation). Colestipol HCl appears to be a safe and effective treatment for hypercholesterolemia; tolerance does not seem to develop."}
{"id": "PMID:1091392", "title": "Interaction of physostigmine and delta-9-tetrahydrocannabinol in man.", "content": "To investigate the hypothesis that delta-9-tetrahydrocannabinol (THC), the major psychoactive ingredient of marihuana, acts by interfering with cholinergic brain mechanisms, 0.75 to 1.25 mg of physostigmine, a centrally active cholinergic drug, was given intravenously to 5 normal volunteers who had ingested 20 to 40 mg of THC 2 hours earlier. Physostigmine decreased the degree of tachycardia and conjunctival injection produced by THC. The major psychologic effects of physostigmine were amplification of the lethargy and somnolence which occur late in the course of THC intoxication. We interpret the lack of physostigmine counteraction of the peak psychologic effects of THC as evidence against the hypothesis that THC acts predominantly by an anticholinergic mechanism.", "contents": "Interaction of physostigmine and delta-9-tetrahydrocannabinol in man. To investigate the hypothesis that delta-9-tetrahydrocannabinol (THC), the major psychoactive ingredient of marihuana, acts by interfering with cholinergic brain mechanisms, 0.75 to 1.25 mg of physostigmine, a centrally active cholinergic drug, was given intravenously to 5 normal volunteers who had ingested 20 to 40 mg of THC 2 hours earlier. Physostigmine decreased the degree of tachycardia and conjunctival injection produced by THC. The major psychologic effects of physostigmine were amplification of the lethargy and somnolence which occur late in the course of THC intoxication. We interpret the lack of physostigmine counteraction of the peak psychologic effects of THC as evidence against the hypothesis that THC acts predominantly by an anticholinergic mechanism."}
{"id": "PMID:1091393", "title": "Hypnotic efficacy of diphenhydramine, methapyrilene, and pentobarbital.", "content": "The antihistamines diphenhydramine and methapyrilene were compared with pentobarbital for hypnotic effect in two Veterans Administration Hospital populations using subjective-response methods. In the first part of the study, 60 mg and 180 mg of pentobarbital were compared with 50 mg and 150 mg of diphenhydramine. A positive dose-response relationship was obtained only for pentobarbital; neither dose of diphenhydramine was significantly different from 60 mg of pentobarbital for any response variable. In the second part of the study, 100 mg of pentobarbital, 50 mg of diphenhydramine, and 50 mg of diphenhydramine, and 50 mg of methapyrilene were compared with placebo. One hundred mg of pentobarbital and 50 mg of diphenhydramine were significantly different from placebo, but 50 mg of methapyrilene was not.", "contents": "Hypnotic efficacy of diphenhydramine, methapyrilene, and pentobarbital. The antihistamines diphenhydramine and methapyrilene were compared with pentobarbital for hypnotic effect in two Veterans Administration Hospital populations using subjective-response methods. In the first part of the study, 60 mg and 180 mg of pentobarbital were compared with 50 mg and 150 mg of diphenhydramine. A positive dose-response relationship was obtained only for pentobarbital; neither dose of diphenhydramine was significantly different from 60 mg of pentobarbital for any response variable. In the second part of the study, 100 mg of pentobarbital, 50 mg of diphenhydramine, and 50 mg of diphenhydramine, and 50 mg of methapyrilene were compared with placebo. One hundred mg of pentobarbital and 50 mg of diphenhydramine were significantly different from placebo, but 50 mg of methapyrilene was not."}
{"id": "PMID:1091395", "title": "Commentary. The normal volunteer in clinical investigation: how rigid should selection criteria be?", "content": "Twenty-nine healthy young male volunteers were screened by history, physical examination, urinalysis, 24 different blood tests, and electrocardiography for admission to a Phase I drug study. The protocol submitted to, and approved by, the FDA listed the normal ranges for the local laboratories and stipulated that the test results for any volunteers accepted should all be within these ranges. During screening 46 abnormal values were found in the laboratory tests. Only 4 subjects had all their test data within \"normal\" range, a result that could have been anticipated simply form the number of tests performed. These findings indicate the need for deciding on realistic protocol criteria for studies involving healthy volunteers.", "contents": "Commentary. The normal volunteer in clinical investigation: how rigid should selection criteria be? Twenty-nine healthy young male volunteers were screened by history, physical examination, urinalysis, 24 different blood tests, and electrocardiography for admission to a Phase I drug study. The protocol submitted to, and approved by, the FDA listed the normal ranges for the local laboratories and stipulated that the test results for any volunteers accepted should all be within these ranges. During screening 46 abnormal values were found in the laboratory tests. Only 4 subjects had all their test data within \"normal\" range, a result that could have been anticipated simply form the number of tests performed. These findings indicate the need for deciding on realistic protocol criteria for studies involving healthy volunteers."}
{"id": "PMID:1091396", "title": "Etorphine in man. I. Subjective effects and suppression of morphine abstinence.", "content": "The effects of etorphine, a potent morphine-like drug, were qualitatively and quantitatively compared to those of morphine. In nondependent subjects, etorphine in doses of 0.025, 0.050, and 0.100 mg produced pupillary constriction and morphine-like subjective effects and euphoria. Etorphine was 500 times as potent as morphine, with a very rapid onset and short duration of action. In morphine-dependent subjects, etorphine suppressed abstinence but for a shorter period than morphine. These studies indicate that in man etorphine is a morphine-like drug with a high abuse potential.", "contents": "Etorphine in man. I. Subjective effects and suppression of morphine abstinence. The effects of etorphine, a potent morphine-like drug, were qualitatively and quantitatively compared to those of morphine. In nondependent subjects, etorphine in doses of 0.025, 0.050, and 0.100 mg produced pupillary constriction and morphine-like subjective effects and euphoria. Etorphine was 500 times as potent as morphine, with a very rapid onset and short duration of action. In morphine-dependent subjects, etorphine suppressed abstinence but for a shorter period than morphine. These studies indicate that in man etorphine is a morphine-like drug with a high abuse potential."}
{"id": "PMID:1091397", "title": "Bioavailability of different lots of digoxin tablets from the same manufacturer.", "content": "The bioavailability of single doses from 4 randomly selected lots of digoxin from a single manufacturer that were recently marketed in the United States was compared. In a crossover study in 6 normal volunteers, serum digoxin concentrations for 0 to 6 hr and urinary glycoside excretion for 24 hr after 0.5-mg doses were measured by radioimmunoassay. The variation in bioavailability between the 4 lots was no greater than that seen when one of them was taken twice. Intersubject variation in areas under the serum concentration-time curves and in the 24-hr urinary digoxin measurements was more than twice that of intrasubject variation. The variability of the urinary digoxin determinations was somewhat less than that of the areas under the serum concentration-time curves.", "contents": "Bioavailability of different lots of digoxin tablets from the same manufacturer. The bioavailability of single doses from 4 randomly selected lots of digoxin from a single manufacturer that were recently marketed in the United States was compared. In a crossover study in 6 normal volunteers, serum digoxin concentrations for 0 to 6 hr and urinary glycoside excretion for 24 hr after 0.5-mg doses were measured by radioimmunoassay. The variation in bioavailability between the 4 lots was no greater than that seen when one of them was taken twice. Intersubject variation in areas under the serum concentration-time curves and in the 24-hr urinary digoxin measurements was more than twice that of intrasubject variation. The variability of the urinary digoxin determinations was somewhat less than that of the areas under the serum concentration-time curves."}
{"id": "PMID:1091398", "title": "Effect of foodstuffs on the absorption of zinc sulfate.", "content": "Single doses of zinc sulfate were given to healthy young volunteers, either in the fasting state or with various types of meals. Dairy products (milk and cheese) and brown bread decreased zinc absorption, as indicated by a significant drop in peak serum zinc levels. Zinc absorption was decreased when zinc was given in the fasting state with the same amounts of purified phosphate or phytate as those found in foods above. Experiments in vitro have shown that zinc is precipitated by phosphate and phytate at pH values close to that of the intestinal lumen. Coffee also seems to inhibit zinc absorption.", "contents": "Effect of foodstuffs on the absorption of zinc sulfate. Single doses of zinc sulfate were given to healthy young volunteers, either in the fasting state or with various types of meals. Dairy products (milk and cheese) and brown bread decreased zinc absorption, as indicated by a significant drop in peak serum zinc levels. Zinc absorption was decreased when zinc was given in the fasting state with the same amounts of purified phosphate or phytate as those found in foods above. Experiments in vitro have shown that zinc is precipitated by phosphate and phytate at pH values close to that of the intestinal lumen. Coffee also seems to inhibit zinc absorption."}
{"id": "PMID:1091417", "title": "Cryptogenic fibrosing alveolitis.", "content": "Clinical, radiographic, and histologic data, together with pulmonary function features, in eleven patients with cryptogenic fibrosing alveolitis, are described in the following study. All these patients presented with a history of exertional dyspnea. Eight of the eleven had radiographic evidence of pleural involvement as manifested by blunting of one or both costophrenic angles. A restrictive defect was present in eight patients, all of whom had x-ray changes showing diffuse involvement. One of the patients presented showed marked improvement in all parameters including a repeat lung biopsy. Immunofluorescent studies of the lung biopsies from six patients revealed no evidence of immunologic damage. Based upon the histologic pattern of pulmonary reaction in the biopsies and the intervals between the onset of symptoms and the time of biopsy, a tenative scheme of temporal evolution of this disease is presented. Lung injury may lead either directly to diffuse interstitial pulmonary fibrosis (DIPF) or lungs may react to injury by an initial alveolar, mural or mixed pattern, all of which might ultimately progress to DIPF. An initial alveolar or mural pattern might change to a mixed pattern.", "contents": "Cryptogenic fibrosing alveolitis. Clinical, radiographic, and histologic data, together with pulmonary function features, in eleven patients with cryptogenic fibrosing alveolitis, are described in the following study. All these patients presented with a history of exertional dyspnea. Eight of the eleven had radiographic evidence of pleural involvement as manifested by blunting of one or both costophrenic angles. A restrictive defect was present in eight patients, all of whom had x-ray changes showing diffuse involvement. One of the patients presented showed marked improvement in all parameters including a repeat lung biopsy. Immunofluorescent studies of the lung biopsies from six patients revealed no evidence of immunologic damage. Based upon the histologic pattern of pulmonary reaction in the biopsies and the intervals between the onset of symptoms and the time of biopsy, a tenative scheme of temporal evolution of this disease is presented. Lung injury may lead either directly to diffuse interstitial pulmonary fibrosis (DIPF) or lungs may react to injury by an initial alveolar, mural or mixed pattern, all of which might ultimately progress to DIPF. An initial alveolar or mural pattern might change to a mixed pattern."}
{"id": "PMID:1091418", "title": "Diphenidol treatment of arrhythmias.", "content": "The antiarrhythmic activity of diphenidol, an antiemetic, has been demonstrated both in electrophysiologic studies of patiens and in experimental arrhythmias in animals. Accordingly, 18 patients with tachyarrhythmias were treated with intravenous diphenidol in doses of 0.5 to 1.5 mg/kg. In six patients with atrial arrhythmias, there was no notable effect. Similarly, 12 patients with premature ventricular contractions were treated and studied. In six of them, ectopic beats were abolished, at least transiently; in three the number of ventricular premature contractions decreased; in two there was no effect; and in one, the number of premature beats was increased. Of the total number of 18 patients, 14 suffered adverse effects related to the central nervous system. These adverse effects were of such severity as to suggest that further studies with diphenidol as an antiarrhythmic are not warranted.", "contents": "Diphenidol treatment of arrhythmias. The antiarrhythmic activity of diphenidol, an antiemetic, has been demonstrated both in electrophysiologic studies of patiens and in experimental arrhythmias in animals. Accordingly, 18 patients with tachyarrhythmias were treated with intravenous diphenidol in doses of 0.5 to 1.5 mg/kg. In six patients with atrial arrhythmias, there was no notable effect. Similarly, 12 patients with premature ventricular contractions were treated and studied. In six of them, ectopic beats were abolished, at least transiently; in three the number of ventricular premature contractions decreased; in two there was no effect; and in one, the number of premature beats was increased. Of the total number of 18 patients, 14 suffered adverse effects related to the central nervous system. These adverse effects were of such severity as to suggest that further studies with diphenidol as an antiarrhythmic are not warranted."}
{"id": "PMID:1091423", "title": "Suture-line recurrence in carcinoma of the colon and rectum.", "content": "Seventy-three patients with adenocarcinoma of the sigmoid, rectosigmoid and rectum were reviewed to determine the incidence of suture-line recurrence. Four patients (5.5 per cent) developed suture-line recurrence despite the use of a number of techniques to reduce this problem. All four recurrences were in patients who had Dukes' C lesions of the rectum. It would appear that such recurrences are not related to implantation factors alone.", "contents": "Suture-line recurrence in carcinoma of the colon and rectum. Seventy-three patients with adenocarcinoma of the sigmoid, rectosigmoid and rectum were reviewed to determine the incidence of suture-line recurrence. Four patients (5.5 per cent) developed suture-line recurrence despite the use of a number of techniques to reduce this problem. All four recurrences were in patients who had Dukes' C lesions of the rectum. It would appear that such recurrences are not related to implantation factors alone."}
{"id": "PMID:1091430", "title": "[Coagulation tests in von Willebrand's disease(author's transl)].", "content": "The diagnosis of von Willebrand's disease can be made by a selected series of easily performed clotting tests. A STudy of 36 patients with the disease (all but one with a mild form) revealed a significantly increased reaction time in the thrombelastogram from 210 plus or minus 33 percent to 270 plus or minus 69 percent (P less than 0.001), and of the partial thromboplastin time from 43 plus or minus 3s to 54 plus or minus 9s (P less than 0.001 than 0.001). Factor VIII activity was corresponding significantly reduced from normal (127 plus or minus 51 percent) to 51 plus or minus 12 percent (P less than 0.001). Other plasma factors and platelet counts were within normal limits. A platelet defect was indicated by an abnormal change in the speed of clot formation (k-time) from 162 plus or minus 39 percent to 341 plus or minus 121 percent (P less than 0.001), maximal thrombelasticity from 121 plus or minus 16 percent to 75 plus or minus 22 percent (P less than 0.001), and marked reduction in platelet-factor 3 liberation from 122 plus or minus 59 percent to 23 plus or minus 16 percent (P less than 0.001). An increase in bleeding time, from 162 plus or minus 41s to 204 plus or minus 128s (P less than 0.05), was not always present. Recently introduced immunological methods for determining factor VIII and factor VIII-associated protein, as well as special platelet-function tests, give further information on the pathogenesis of the bleeding disease, but their complexity restricts them to special laboratories.", "contents": "[Coagulation tests in von Willebrand's disease(author's transl)]. The diagnosis of von Willebrand's disease can be made by a selected series of easily performed clotting tests. A STudy of 36 patients with the disease (all but one with a mild form) revealed a significantly increased reaction time in the thrombelastogram from 210 plus or minus 33 percent to 270 plus or minus 69 percent (P less than 0.001), and of the partial thromboplastin time from 43 plus or minus 3s to 54 plus or minus 9s (P less than 0.001 than 0.001). Factor VIII activity was corresponding significantly reduced from normal (127 plus or minus 51 percent) to 51 plus or minus 12 percent (P less than 0.001). Other plasma factors and platelet counts were within normal limits. A platelet defect was indicated by an abnormal change in the speed of clot formation (k-time) from 162 plus or minus 39 percent to 341 plus or minus 121 percent (P less than 0.001), maximal thrombelasticity from 121 plus or minus 16 percent to 75 plus or minus 22 percent (P less than 0.001), and marked reduction in platelet-factor 3 liberation from 122 plus or minus 59 percent to 23 plus or minus 16 percent (P less than 0.001). An increase in bleeding time, from 162 plus or minus 41s to 204 plus or minus 128s (P less than 0.05), was not always present. Recently introduced immunological methods for determining factor VIII and factor VIII-associated protein, as well as special platelet-function tests, give further information on the pathogenesis of the bleeding disease, but their complexity restricts them to special laboratories."}
{"id": "PMID:1091431", "title": "[Glossopharyngeal neuralgia with cardiac arrhythmias (author's transl)].", "content": "In a 68-year-old female patient glossopharyngeal neuralgia of unknown origin occurred accompained by cardiac arrhythmias (bradycardia to asystole) and loss of consciousness. Subcutaneous atropine injections improved bradycardia but did not influence attacks of pain. Complete and permanent freedom from symptoms was established only after treatment with carbamazepine (Tegretal) 200 mg t.d.s.", "contents": "[Glossopharyngeal neuralgia with cardiac arrhythmias (author's transl)]. In a 68-year-old female patient glossopharyngeal neuralgia of unknown origin occurred accompained by cardiac arrhythmias (bradycardia to asystole) and loss of consciousness. Subcutaneous atropine injections improved bradycardia but did not influence attacks of pain. Complete and permanent freedom from symptoms was established only after treatment with carbamazepine (Tegretal) 200 mg t.d.s."}
{"id": "PMID:1091434", "title": "[Studies on the bio-availability of tolbutamide (author's transl)].", "content": "Two different batches of Rastinon 1,0 Hoechst were administered, a year apart, to two groups of healthy subjects (ten and six men, respectively) without any difference in effect on blood sugar being found. The blood sugar concentration was measured in six healthy men before and after oral administration of 1,000 mg tolbutamide (as Rastinon 1,0 Hoechst or tolbutamid tablets Ratiopharm), in a blind test. After tolbutamide Ratiopharm, the area under the blood sugar concentration-time curves was only 29 percent (0-4 hafter medication) and 32 percent (4-8 h after medication) of that after Rastinon 1,0 (P 2alpha less than 0.01), with marked scatter between individuals. Maximal serum concentration was 80 percent below that after Rastinon. The first measurable value was reached 0.8 plus or minus 0.2 h after medication of Rastinon and 3.6 plus or minus 0.8 h after tolbutamide Ratiopharm. The areas under the serum concentration under the curves after tolbutamide Ratiopharm were only 16 percent (0-4 h after medication) and 19 percent (0-8 h after medication) of those after Rastinon (P 2alpha less than 0.05 and 0.01, respectively). The differences demonstrate that tolbutamide Ratiopharm tablets and Rastinon 1,0 Hoechst are not equivalent biologically and therapeutically.", "contents": "[Studies on the bio-availability of tolbutamide (author's transl)]. Two different batches of Rastinon 1,0 Hoechst were administered, a year apart, to two groups of healthy subjects (ten and six men, respectively) without any difference in effect on blood sugar being found. The blood sugar concentration was measured in six healthy men before and after oral administration of 1,000 mg tolbutamide (as Rastinon 1,0 Hoechst or tolbutamid tablets Ratiopharm), in a blind test. After tolbutamide Ratiopharm, the area under the blood sugar concentration-time curves was only 29 percent (0-4 hafter medication) and 32 percent (4-8 h after medication) of that after Rastinon 1,0 (P 2alpha less than 0.01), with marked scatter between individuals. Maximal serum concentration was 80 percent below that after Rastinon. The first measurable value was reached 0.8 plus or minus 0.2 h after medication of Rastinon and 3.6 plus or minus 0.8 h after tolbutamide Ratiopharm. The areas under the serum concentration under the curves after tolbutamide Ratiopharm were only 16 percent (0-4 h after medication) and 19 percent (0-8 h after medication) of those after Rastinon (P 2alpha less than 0.05 and 0.01, respectively). The differences demonstrate that tolbutamide Ratiopharm tablets and Rastinon 1,0 Hoechst are not equivalent biologically and therapeutically."}
{"id": "PMID:1091437", "title": "[Abnormal hypothalamic-hypophyseal regulation as a new endocrine sign in the testicular feminisation syndrome (author's transl)].", "content": "In a 38-year-old woman with the testicular feminisation syndrome (TFS) there were increased serum levels of LH and FSH, despite normal testosterone levels, ranging from 808 to 1330 ng/dl. The patient exhibited complete end-organ resistance to androgens, as well as decreased free testosterone fraction of 1.5 percent. On stimulation with LH-RH there was enhanced LH reaction to 15 ng/ml, while FSH levels remained unchanged. Fluoxymesterone did not suppress LH and testosterone, as it did in normal controls. But the functional Leydig-cell reserve could be normally stimulated with HCG. Our results apparently confirm the suspected reduction in sensitivity of the hypothalamic-pituitary axis to androgens and suggest that this plays an important part in the pathogenesis of TES.", "contents": "[Abnormal hypothalamic-hypophyseal regulation as a new endocrine sign in the testicular feminisation syndrome (author's transl)]. In a 38-year-old woman with the testicular feminisation syndrome (TFS) there were increased serum levels of LH and FSH, despite normal testosterone levels, ranging from 808 to 1330 ng/dl. The patient exhibited complete end-organ resistance to androgens, as well as decreased free testosterone fraction of 1.5 percent. On stimulation with LH-RH there was enhanced LH reaction to 15 ng/ml, while FSH levels remained unchanged. Fluoxymesterone did not suppress LH and testosterone, as it did in normal controls. But the functional Leydig-cell reserve could be normally stimulated with HCG. Our results apparently confirm the suspected reduction in sensitivity of the hypothalamic-pituitary axis to androgens and suggest that this plays an important part in the pathogenesis of TES."}
{"id": "PMID:1091455", "title": "[The cast structure after vacuum-pressure casting and centrifugal casting].", "content": "Evaluation of polished sections revealed the following: 1. Grain size in marginal areas of the crown is, in general, smaller than it is in the occlusal region. 2. Grain size varies depending on the casting method applied. Differences in hardness were found only to a limited extent. They were not always in line with the grain structure. This phenomenon deviating from the rule cannot satisfactorily be explained. The piping distribution was in accordance with observations made so far.", "contents": "[The cast structure after vacuum-pressure casting and centrifugal casting]. Evaluation of polished sections revealed the following: 1. Grain size in marginal areas of the crown is, in general, smaller than it is in the occlusal region. 2. Grain size varies depending on the casting method applied. Differences in hardness were found only to a limited extent. They were not always in line with the grain structure. This phenomenon deviating from the rule cannot satisfactorily be explained. The piping distribution was in accordance with observations made so far."}
{"id": "PMID:1091456", "title": "[Effect of thermal expansion on the accuracy of fit of cast crowns].", "content": "Extent and direction of dimensional changes in the finished cast crown resulting from the thermic expansion of the wax model are determined as a function of the time the crown was subjected to tempering as a function of tempering temperature. Expansion of the wax model does not come up to the expectation of preventing a reduction of the cast object.", "contents": "[Effect of thermal expansion on the accuracy of fit of cast crowns]. Extent and direction of dimensional changes in the finished cast crown resulting from the thermic expansion of the wax model are determined as a function of the time the crown was subjected to tempering as a function of tempering temperature. Expansion of the wax model does not come up to the expectation of preventing a reduction of the cast object."}
{"id": "PMID:1091460", "title": "[Gingival hyperplasia in bar joint prostheses].", "content": "The occurrence of mucosal hyperplasias under 57 bars (according to Dolder) was examined. It was found that apart from the time factor, cleaning habits of the patient and the construction of the dentures played an important role. Short bars are more difficult to clean than those with wide distances between the abutments. The influence of additional factors can only be demonstrated in combination with other factors, this suggests rather complex connections.", "contents": "[Gingival hyperplasia in bar joint prostheses]. The occurrence of mucosal hyperplasias under 57 bars (according to Dolder) was examined. It was found that apart from the time factor, cleaning habits of the patient and the construction of the dentures played an important role. Short bars are more difficult to clean than those with wide distances between the abutments. The influence of additional factors can only be demonstrated in combination with other factors, this suggests rather complex connections."}
{"id": "PMID:1091461", "title": "[Bacteriological studies of removable splints and dentures].", "content": "The risk of germ contamination through already used dental replacement cannot or only with difficulties be solved by disinfection. As to new dental replacements, which may constitute a germ vehicle between the dental laboratory and the dental office, specific consequences can be drawn. It is tried to explain where contamination may start so that such contamination can be prevented.", "contents": "[Bacteriological studies of removable splints and dentures]. The risk of germ contamination through already used dental replacement cannot or only with difficulties be solved by disinfection. As to new dental replacements, which may constitute a germ vehicle between the dental laboratory and the dental office, specific consequences can be drawn. It is tried to explain where contamination may start so that such contamination can be prevented."}
{"id": "PMID:1091463", "title": "[Prosthetic rehabilitation of dysgnathic patients].", "content": "There are only few reports on the prosthetical care of patients with dysgnathias. Thus, it seems appropriate first to delimit the field by well-defined terms from maxillo-facial surgery and orthodontics. Reichenbach, K\u00f6le, and Br\u00fcckl introduced the term \"principal symptom\" (\"Leitsymptom\") for the most significant dysgnathic finding. With a view to prosthetical aspects, this term is broadened to include primary and secondary dysgnathias, after several proposals of nomenclature were made. It is reported on the number of dysgnathic patients needing treatment. The description of some of the goals aimed at by prosthetical care concludes the paper.", "contents": "[Prosthetic rehabilitation of dysgnathic patients]. There are only few reports on the prosthetical care of patients with dysgnathias. Thus, it seems appropriate first to delimit the field by well-defined terms from maxillo-facial surgery and orthodontics. Reichenbach, K\u00f6le, and Br\u00fcckl introduced the term \"principal symptom\" (\"Leitsymptom\") for the most significant dysgnathic finding. With a view to prosthetical aspects, this term is broadened to include primary and secondary dysgnathias, after several proposals of nomenclature were made. It is reported on the number of dysgnathic patients needing treatment. The description of some of the goals aimed at by prosthetical care concludes the paper."}
{"id": "PMID:1091464", "title": "[Special problems in the prosthetic rehabilitation of deep bite].", "content": "In cases of deep bite where the lower front teeth contact the palatal gingiva or where the upper teeth contact the labial gingiva of the lower jaw, the attempt to improve the situation by grinding-in the teeth was always crowned with temporary success only. The question as to whether the front teeth may be maintained determines further measures to be taken. If they can and if they are to be kept it is necessary to change the vertical relation of practically all teeth by safeguarding the antagonistic contract also in the front area. Therapeutical means and risks involved are considerable. If the teeth concerned have to be removed, plastic correction of the alveolar ridge is necessary before bridges and removable dentures are fitted in. This solution is even less satisfactory and disregards the significance of natural teeth for the well-being of the patient.", "contents": "[Special problems in the prosthetic rehabilitation of deep bite]. In cases of deep bite where the lower front teeth contact the palatal gingiva or where the upper teeth contact the labial gingiva of the lower jaw, the attempt to improve the situation by grinding-in the teeth was always crowned with temporary success only. The question as to whether the front teeth may be maintained determines further measures to be taken. If they can and if they are to be kept it is necessary to change the vertical relation of practically all teeth by safeguarding the antagonistic contract also in the front area. Therapeutical means and risks involved are considerable. If the teeth concerned have to be removed, plastic correction of the alveolar ridge is necessary before bridges and removable dentures are fitted in. This solution is even less satisfactory and disregards the significance of natural teeth for the well-being of the patient."}
{"id": "PMID:1091466", "title": "[Control studies on periodontal and periodontal-gingival retention of partial prosthesis].", "content": "Follow-ups of 722 removable partial dentures that have been used for an average 5.2 years reveal that the way they were used and the type of balancing as well as the construction of the denture have an influence on the inflammation of the denture-bearing area and the loosening of the abutment teeth. In this investigation it was not possible to demonstrate the influence of daily personal oral hygience.", "contents": "[Control studies on periodontal and periodontal-gingival retention of partial prosthesis]. Follow-ups of 722 removable partial dentures that have been used for an average 5.2 years reveal that the way they were used and the type of balancing as well as the construction of the denture have an influence on the inflammation of the denture-bearing area and the loosening of the abutment teeth. In this investigation it was not possible to demonstrate the influence of daily personal oral hygience."}
{"id": "PMID:1091467", "title": "[Follow-up studies on dentures in patients with teeth in both jaws which do not occlude (Group C-1 according to Eichner)].", "content": "Follow-ups in class C 1 patients who underwent prosthetical treatment seemed indicated since little was known about the chances of success. It was possible to examine 20 patients of this groups mainly with regard to objective functional efficiency of the dental replacement worn from 1 to 8 years. Furthermore, articulation occlusion, relationship of upper and lower jaws, and frequency of required repairs were checked. Purely gingivally borne prostheses as well as periodontally supported and gingivally borne dentures had the same rate of success. The mixed form, i.e. gingivally borne in the upper jaw and periodontally supported and gingivally borne in the lower jaw, seemed to be less favorable due to differing load tolerances.", "contents": "[Follow-up studies on dentures in patients with teeth in both jaws which do not occlude (Group C-1 according to Eichner)]. Follow-ups in class C 1 patients who underwent prosthetical treatment seemed indicated since little was known about the chances of success. It was possible to examine 20 patients of this groups mainly with regard to objective functional efficiency of the dental replacement worn from 1 to 8 years. Furthermore, articulation occlusion, relationship of upper and lower jaws, and frequency of required repairs were checked. Purely gingivally borne prostheses as well as periodontally supported and gingivally borne dentures had the same rate of success. The mixed form, i.e. gingivally borne in the upper jaw and periodontally supported and gingivally borne in the lower jaw, seemed to be less favorable due to differing load tolerances."}
{"id": "PMID:1091468", "title": "[Current studies on shape changes of total prostheses in relation to the method of their construction].", "content": "It was shown that marginal gaps occurring after the preparation of upper complete dentures vary depending on the material or method applied and that in all tested materials the gaps become smaller again during subsequent swelling, but are not fully compensated.", "contents": "[Current studies on shape changes of total prostheses in relation to the method of their construction]. It was shown that marginal gaps occurring after the preparation of upper complete dentures vary depending on the material or method applied and that in all tested materials the gaps become smaller again during subsequent swelling, but are not fully compensated."}
{"id": "PMID:1091469", "title": "[Shape retention of plastic prostheses manufactured by the pouning method].", "content": "In investigations was shown that PMMA dentures fabricated by injection molding have a better dimensional stability than polycarbonate dentures (Andoran) and are slightly better than packed Paladon 65 dentures. When using the injection molding technique a slight increase in occlusion could not be prevented in any of the dentures.", "contents": "[Shape retention of plastic prostheses manufactured by the pouning method]. In investigations was shown that PMMA dentures fabricated by injection molding have a better dimensional stability than polycarbonate dentures (Andoran) and are slightly better than packed Paladon 65 dentures. When using the injection molding technique a slight increase in occlusion could not be prevented in any of the dentures."}
{"id": "PMID:1091470", "title": "[Studies on the retention of total lower dentures in relation to the time of wearing].", "content": "Clinical experience in the prosthetical care of edentulous patients shows that primarily patients wearing dentures for the first time express the desire, after a few months, to have original denture adhesion restored. Especially for complete lower dentures diminishing adhesion is unfavorable. Measurements regarding the adhesive strength of complete lower dentures of 34 patients showed that adhesive strength is decreasing. This was measured and statistically evaluated in this investigation. 33 percent of the original adhesive strength is lost within 6 months. With originally strong adhesion (2, 000 p) this reduction is more drastic than with originally small adhesion (200 p). It was not possible to establish that adhesive strength is declining preferably as a result of a specific anatomic situation.", "contents": "[Studies on the retention of total lower dentures in relation to the time of wearing]. Clinical experience in the prosthetical care of edentulous patients shows that primarily patients wearing dentures for the first time express the desire, after a few months, to have original denture adhesion restored. Especially for complete lower dentures diminishing adhesion is unfavorable. Measurements regarding the adhesive strength of complete lower dentures of 34 patients showed that adhesive strength is decreasing. This was measured and statistically evaluated in this investigation. 33 percent of the original adhesive strength is lost within 6 months. With originally strong adhesion (2, 000 p) this reduction is more drastic than with originally small adhesion (200 p). It was not possible to establish that adhesive strength is declining preferably as a result of a specific anatomic situation."}
{"id": "PMID:1091471", "title": "Synthesis of DNA in isolated pancreatic islets maintained in tissue culture.", "content": "The DNA synthesis of isolated pancreatic islets has been determined after culture in vitro of the islets for 5 days in media containing either various glucose concentrations or tolbutamide or cortisone. Only glucose stimulated the replication of DNA, while cortisone was inhibitory and tolbutamide without effect. It is concluded that glucose by itself stimulates the mitotic rate of the islet cells and that the corresponding effect of steroids in vivo may be mediated through the ensuing hyperglycemia.", "contents": "Synthesis of DNA in isolated pancreatic islets maintained in tissue culture. The DNA synthesis of isolated pancreatic islets has been determined after culture in vitro of the islets for 5 days in media containing either various glucose concentrations or tolbutamide or cortisone. Only glucose stimulated the replication of DNA, while cortisone was inhibitory and tolbutamide without effect. It is concluded that glucose by itself stimulates the mitotic rate of the islet cells and that the corresponding effect of steroids in vivo may be mediated through the ensuing hyperglycemia."}
{"id": "PMID:1091472", "title": "Failure to detect luteinizing hormone-releasing hormone in third ventricle cerebral spinal fluid under a variety of experimental conditions.", "content": "Cerebral spinal fluid (CSF) was examined for the presence of luteinizing hormone-releasing hormone (LH-RH) at times when copious amounts of LH were being secreted by the adenohypophysis. A \"push-pull\" apparatus was designed which permitted the perfusion of artificial CSF through the 3rd ventricle from the region of the medial preoptic area (MPOA) to the site of recollection adjacent to the median eminence (ME). It was observed on radioimmunoassay that LH-RH concentrations were low in CSF and peripheral plasma of Nembutal-blocked proestrous rats. Intraarterial injections of LH-RH did not increase CSF concentrations of this decapeptide, although a significant release of pituitary LH occurred. Further, electrochemical stimulation of the MPOA also elevated plasma LH without altering CSF-LH-RH levels. When synthetic LH-RH, dissolved in artificial CSF, was perfused through the 3rd ventricle, plasma LH levels rose dramatically, and a significant decrease in the concentration of LH-RH in the CSF efflux was observed on collection and assay. CSF collected from MPOA-stimulated rats also was tested for biologically active LH-RH by its reinjection into Nembutal-blocked proestrous recipients. While such injections failed to induce pituitary LH release, it was observed that LH-RH, when mixed with artificial CSF, and allowed to stand at room temperature (24 C), also lost all biological potency even though immunoreactivity was retained. The results suggest that CSF does not serve as a vehicle for transport of LH-RH to the median eminence under physiological conditions.", "contents": "Failure to detect luteinizing hormone-releasing hormone in third ventricle cerebral spinal fluid under a variety of experimental conditions. Cerebral spinal fluid (CSF) was examined for the presence of luteinizing hormone-releasing hormone (LH-RH) at times when copious amounts of LH were being secreted by the adenohypophysis. A \"push-pull\" apparatus was designed which permitted the perfusion of artificial CSF through the 3rd ventricle from the region of the medial preoptic area (MPOA) to the site of recollection adjacent to the median eminence (ME). It was observed on radioimmunoassay that LH-RH concentrations were low in CSF and peripheral plasma of Nembutal-blocked proestrous rats. Intraarterial injections of LH-RH did not increase CSF concentrations of this decapeptide, although a significant release of pituitary LH occurred. Further, electrochemical stimulation of the MPOA also elevated plasma LH without altering CSF-LH-RH levels. When synthetic LH-RH, dissolved in artificial CSF, was perfused through the 3rd ventricle, plasma LH levels rose dramatically, and a significant decrease in the concentration of LH-RH in the CSF efflux was observed on collection and assay. CSF collected from MPOA-stimulated rats also was tested for biologically active LH-RH by its reinjection into Nembutal-blocked proestrous recipients. While such injections failed to induce pituitary LH release, it was observed that LH-RH, when mixed with artificial CSF, and allowed to stand at room temperature (24 C), also lost all biological potency even though immunoreactivity was retained. The results suggest that CSF does not serve as a vehicle for transport of LH-RH to the median eminence under physiological conditions."}
{"id": "PMID:1091473", "title": "Effects of porcine stalk median eminence and prostaglandin E2 on rat growth hormone secretion in vivo and their inhibition by somatostatin.", "content": "The studies described were performed to develop a technically simple, yet sensitive in vivo model for growth hormone (GH) releasing activity in porcine stalk median eminence (pSME) extracts, to compare the GH releasing effects of pSME with those of prostaglandin E2 (PGE2), and to study the effect of somatostatin (SRIF) on the above stimuli. The use of the one day estrogen-primed male rat in conjunction with intracarotid injection of test materials provided a model sensitive to the injection of one pSME. Neither increasing the duration of estrogen pre-treatment nor reserpine resulted in a greater response. The GH releasing effects of pSME were directly related to the preinjection GH level. Two successive injections of pSME at 30 minute intervals evoked similar responses. In contrast, PGE2 effects were not potentiated by estrogen pre-treatment and were independent of the preinjection GH level. The GH releasing effect of pSME was not related to its content of TRH or K+. Extracts of porcine cerebral cortex also contained GH releasing activity, although at a lower concentration than in pSME. Somatostatin inhibited the GH releasing effects of pSME, PGE2, and cerebral cortex extract. These results provide evidence for the direct inhibitory effect of SRIF on GH secretion in vivo and suggest that SRIF is capable of blocking a variety of different stimuli to GH release.", "contents": "Effects of porcine stalk median eminence and prostaglandin E2 on rat growth hormone secretion in vivo and their inhibition by somatostatin. The studies described were performed to develop a technically simple, yet sensitive in vivo model for growth hormone (GH) releasing activity in porcine stalk median eminence (pSME) extracts, to compare the GH releasing effects of pSME with those of prostaglandin E2 (PGE2), and to study the effect of somatostatin (SRIF) on the above stimuli. The use of the one day estrogen-primed male rat in conjunction with intracarotid injection of test materials provided a model sensitive to the injection of one pSME. Neither increasing the duration of estrogen pre-treatment nor reserpine resulted in a greater response. The GH releasing effects of pSME were directly related to the preinjection GH level. Two successive injections of pSME at 30 minute intervals evoked similar responses. In contrast, PGE2 effects were not potentiated by estrogen pre-treatment and were independent of the preinjection GH level. The GH releasing effect of pSME was not related to its content of TRH or K+. Extracts of porcine cerebral cortex also contained GH releasing activity, although at a lower concentration than in pSME. Somatostatin inhibited the GH releasing effects of pSME, PGE2, and cerebral cortex extract. These results provide evidence for the direct inhibitory effect of SRIF on GH secretion in vivo and suggest that SRIF is capable of blocking a variety of different stimuli to GH release."}
{"id": "PMID:1091474", "title": "Augmentation of pituitary responsiveness to luteinizing hormone/follicle stimulating hormone-releasing factor (LH-RF) as a result of acute ovariectomy in the four-day cyclic rat.", "content": "Within 6 h of ovariectomy in adult rats with 4-day estrous cycles, plasma FSH titers were significantly elevated above those of sham-operated controls at all stages of the estrous cycle, whereas plasma LH concentrations were raised by ovariectomy only during diestrus day 2, proestrus or estrus. A single intravenous injection of a partially purified extract of ovine stalk-median eminence tissue (LH-RF) into sham-operated control rats or incubation of pituitaries from similar rats with the same releasing factor extract elevated LH and FSH output at all stages of the cycle. This response to LH-RF was related to the stage of the estrous cycle, maximal pituitary responsiveness occurring on proestrus. Ovariectomy 6 h before administration of LH-RF significantly augmented pituitary LH and FSH responsiveness both in vivo and in vitro. This elevation in hypophyseal responsiveness observed in vitro followed a cyclic pattern which could be superimposed on the normal cyclic changes in pituitary responsiveness, whereas the cyclic increase in pituitary LH responsiveness to LH-RF in vivo following acute ovariectomy was first detectable 16 to 18 h earlier than in intact controls, that is, on late diestrus day 2 rather than on the morning of proestrus. These results indicate that acute ovariectomy can augment pituitary responsiveness to LH-RF and can also advance the onset of the cyclic augmentation of pituitary responsiveness to LH-RF during the 24 h immediately preceding the ovulatory gonadotropin surge. Possible explanations for these effects are discussed.", "contents": "Augmentation of pituitary responsiveness to luteinizing hormone/follicle stimulating hormone-releasing factor (LH-RF) as a result of acute ovariectomy in the four-day cyclic rat. Within 6 h of ovariectomy in adult rats with 4-day estrous cycles, plasma FSH titers were significantly elevated above those of sham-operated controls at all stages of the estrous cycle, whereas plasma LH concentrations were raised by ovariectomy only during diestrus day 2, proestrus or estrus. A single intravenous injection of a partially purified extract of ovine stalk-median eminence tissue (LH-RF) into sham-operated control rats or incubation of pituitaries from similar rats with the same releasing factor extract elevated LH and FSH output at all stages of the cycle. This response to LH-RF was related to the stage of the estrous cycle, maximal pituitary responsiveness occurring on proestrus. Ovariectomy 6 h before administration of LH-RF significantly augmented pituitary LH and FSH responsiveness both in vivo and in vitro. This elevation in hypophyseal responsiveness observed in vitro followed a cyclic pattern which could be superimposed on the normal cyclic changes in pituitary responsiveness, whereas the cyclic increase in pituitary LH responsiveness to LH-RF in vivo following acute ovariectomy was first detectable 16 to 18 h earlier than in intact controls, that is, on late diestrus day 2 rather than on the morning of proestrus. These results indicate that acute ovariectomy can augment pituitary responsiveness to LH-RF and can also advance the onset of the cyclic augmentation of pituitary responsiveness to LH-RF during the 24 h immediately preceding the ovulatory gonadotropin surge. Possible explanations for these effects are discussed."}
{"id": "PMID:1091475", "title": "An in vivo assay for anti-LH-RH and anti-FSH-RH activity of inhibitory analogues of LH-RH.", "content": "A convenient and sensitive method for testing anti-LH- and FSH-releasing activities of antagonists of LH-RH was devised. The peptides to be tested were injected subcutaneously together with synthetic LH-releasing hormone (LH-RH) in immature male rats. Serum LH and FSH concentrations were measured 30 min later by radioimmunoassay. In this system, [DesHis2]-LH-RH failed to block the pituitary response to LH-RH in agreement with previous data. The administration of [DesHis2, desGly10]-LH-RH ethylamide, [Des-His2, Leu3, des Gly10]-LH-RH ethylamide, [Des-His2, D-Ala6, des-Gly10]-LH-RH ethylamide, and [DESHis2, D-Ala6]-LH-RH in doses of 200 mug/rat, and [DesHis2, Leu3, D-Ala, desGly10]-LH-RH ethylamide in doses ranging from 100-400 mug/rat caused a significant inhibition of LH-RH-induced release of LH and FSH. On the other hand, [DesHis2, desGly10]-LH-RH propylamide, and [Leu2, Leu3, D-Ala6, desGly10]-LH-RH ethylamide failed to block the response to LH-RH. Among the peptides tested, [DesHis2, d-ala6, desGly10]-LH-RH etylamide, [Leu2, Leu3, D-Ala, desGly10]-LH-RH ethylamide in doses of 200 mug and [DesHis2, Leu3, D-Ala6, desGly10]-LH-RH ethylamide in a dose of 400 mug/rat showed some intrinsic LH and FSH-releasing activities. The method described here is simple and convenient for studying the anti-LH-RH release activity of inhibitory analogues of LH-RH, and also enabled us to demonstrate for the first time an in vivo inhibition of FSH release by these analogues.", "contents": "An in vivo assay for anti-LH-RH and anti-FSH-RH activity of inhibitory analogues of LH-RH. A convenient and sensitive method for testing anti-LH- and FSH-releasing activities of antagonists of LH-RH was devised. The peptides to be tested were injected subcutaneously together with synthetic LH-releasing hormone (LH-RH) in immature male rats. Serum LH and FSH concentrations were measured 30 min later by radioimmunoassay. In this system, [DesHis2]-LH-RH failed to block the pituitary response to LH-RH in agreement with previous data. The administration of [DesHis2, desGly10]-LH-RH ethylamide, [Des-His2, Leu3, des Gly10]-LH-RH ethylamide, [Des-His2, D-Ala6, des-Gly10]-LH-RH ethylamide, and [DESHis2, D-Ala6]-LH-RH in doses of 200 mug/rat, and [DesHis2, Leu3, D-Ala, desGly10]-LH-RH ethylamide in doses ranging from 100-400 mug/rat caused a significant inhibition of LH-RH-induced release of LH and FSH. On the other hand, [DesHis2, desGly10]-LH-RH propylamide, and [Leu2, Leu3, D-Ala6, desGly10]-LH-RH ethylamide failed to block the response to LH-RH. Among the peptides tested, [DesHis2, d-ala6, desGly10]-LH-RH etylamide, [Leu2, Leu3, D-Ala, desGly10]-LH-RH ethylamide in doses of 200 mug and [DesHis2, Leu3, D-Ala6, desGly10]-LH-RH ethylamide in a dose of 400 mug/rat showed some intrinsic LH and FSH-releasing activities. The method described here is simple and convenient for studying the anti-LH-RH release activity of inhibitory analogues of LH-RH, and also enabled us to demonstrate for the first time an in vivo inhibition of FSH release by these analogues."}
{"id": "PMID:1091476", "title": "In vitro release of lutenizing hormone from anterior pituitary fragments superfused with constant or pulsatile amounts of luteinizing hormone-releasing factor.", "content": "Anterior pituitary fragments from ovariectomized rats were superfused with medium to which lutenising hormone-releasing factor (LH-RF) was added for either prolonged or short intervals. Samples of superfusate were collected at 2-min intervals before and after the onset of LH-RF administration. In control experiments without anterior pituitary fragments, infusions of constant amounts of NIAMDD-Rat LH were made into the superfusion chamber. The superfusate was collected in all experiments and luteinizing hormone (LH) levels measured by radioimmunoassay. LH release was increased 100-200% following superfusion with concentrations of LH-RF ranging from 10-9 to 10-5M. The percent coefficient of variation (%CV) was determined for LH to quantitate fluctuations in hormone release. A nonepisodic pattern of LH release was obtained prior to LH-RF administration and during superfusion with constant levels of LH-RF. There was no significant difference in the %CV in these periods as compared to that obtained during infusion of constant amounts of LH into the superfusion chamber. However stimulation by LH-RF for only 2-5 min caused episodes of LH release lasting 30-40 min. The %CV of superfusate LH during these pulsatile stimulation studies was significantly increased compared to that obtained during superfusion with constant levels of LH-RF. These results suggest that the pituitary is not the site of origin of the mechanism underlying episodic release of LH, but can release LH in a pulsatile manner when driven by pulsatile LH-RF stimulation.", "contents": "In vitro release of lutenizing hormone from anterior pituitary fragments superfused with constant or pulsatile amounts of luteinizing hormone-releasing factor. Anterior pituitary fragments from ovariectomized rats were superfused with medium to which lutenising hormone-releasing factor (LH-RF) was added for either prolonged or short intervals. Samples of superfusate were collected at 2-min intervals before and after the onset of LH-RF administration. In control experiments without anterior pituitary fragments, infusions of constant amounts of NIAMDD-Rat LH were made into the superfusion chamber. The superfusate was collected in all experiments and luteinizing hormone (LH) levels measured by radioimmunoassay. LH release was increased 100-200% following superfusion with concentrations of LH-RF ranging from 10-9 to 10-5M. The percent coefficient of variation (%CV) was determined for LH to quantitate fluctuations in hormone release. A nonepisodic pattern of LH release was obtained prior to LH-RF administration and during superfusion with constant levels of LH-RF. There was no significant difference in the %CV in these periods as compared to that obtained during infusion of constant amounts of LH into the superfusion chamber. However stimulation by LH-RF for only 2-5 min caused episodes of LH release lasting 30-40 min. The %CV of superfusate LH during these pulsatile stimulation studies was significantly increased compared to that obtained during superfusion with constant levels of LH-RF. These results suggest that the pituitary is not the site of origin of the mechanism underlying episodic release of LH, but can release LH in a pulsatile manner when driven by pulsatile LH-RF stimulation."}
{"id": "PMID:1091477", "title": "Insulin biosynthesis: the monoaminergic mechanisms and the specificity of \"glucoreceptor\".", "content": "Islets of Langerhans, isolated from the rat, did not synthesize insulin in the presence of L-glucose, fructose, galactose, 3-0-methyl glucose or sorbitol. A small amount of insulin was synthesized in the presence of glucosamine. Addition of caffeine to these compounds did not enhance the biosynthesis of insulin. It is suggested that the specificity of the membrane surface \"glucoreceptor\", if it exists, may be rather narrow, at least with respect to insulin biosynthesis. Serotonin, dopamine and isoproterenol did not inhibit or enhance insulin biosynthesis induced by glucose. Propranolol also failed to modify insulin synthesis in the absence or presence of isoproterenol. It is concluded that the monoaminergic mechanisms do not affect insulin biosynthesis in spite of their significant regulatory influence on insulin release. Methysergide, at the concentration reported to potentiate insulin release induced by glucose and tolbutamide in rabbit, strongly inhibited insulin biosynthesis.", "contents": "Insulin biosynthesis: the monoaminergic mechanisms and the specificity of \"glucoreceptor\". Islets of Langerhans, isolated from the rat, did not synthesize insulin in the presence of L-glucose, fructose, galactose, 3-0-methyl glucose or sorbitol. A small amount of insulin was synthesized in the presence of glucosamine. Addition of caffeine to these compounds did not enhance the biosynthesis of insulin. It is suggested that the specificity of the membrane surface \"glucoreceptor\", if it exists, may be rather narrow, at least with respect to insulin biosynthesis. Serotonin, dopamine and isoproterenol did not inhibit or enhance insulin biosynthesis induced by glucose. Propranolol also failed to modify insulin synthesis in the absence or presence of isoproterenol. It is concluded that the monoaminergic mechanisms do not affect insulin biosynthesis in spite of their significant regulatory influence on insulin release. Methysergide, at the concentration reported to potentiate insulin release induced by glucose and tolbutamide in rabbit, strongly inhibited insulin biosynthesis."}
{"id": "PMID:1091478", "title": "Chromatographic evidence for the existence of another species of luteinizing hormone-releasing factor (LRF).", "content": "Fractionation of ultrafiltrates of rat hypothalamic extracts has been performed on Sephadex LH-20 in dimethylformamide/water. The fractions were examined for their content of luteinizing hormone-releasing activity by bioassay in vitro utilizing LH radioimmunoassay, and also for their content of LRF decapeptide-like material by direct radioimmunoassay. Each method revealed the same pattern of activity. This consisted of a major peak whose elution position coincided with that of authentic decapeptide, and a minor region which closely preceded it, often taking the form of a shoulder on the leading edge of the main peak. The active component in the minor peak could be completely resolved by repeated filtration in the same system thus indicating that it represented another gonadotropin-releasing species rather than a chromatographic artefact although its biological and immunological activities indicate that its structure must resemble closely that of the decapeptide.", "contents": "Chromatographic evidence for the existence of another species of luteinizing hormone-releasing factor (LRF). Fractionation of ultrafiltrates of rat hypothalamic extracts has been performed on Sephadex LH-20 in dimethylformamide/water. The fractions were examined for their content of luteinizing hormone-releasing activity by bioassay in vitro utilizing LH radioimmunoassay, and also for their content of LRF decapeptide-like material by direct radioimmunoassay. Each method revealed the same pattern of activity. This consisted of a major peak whose elution position coincided with that of authentic decapeptide, and a minor region which closely preceded it, often taking the form of a shoulder on the leading edge of the main peak. The active component in the minor peak could be completely resolved by repeated filtration in the same system thus indicating that it represented another gonadotropin-releasing species rather than a chromatographic artefact although its biological and immunological activities indicate that its structure must resemble closely that of the decapeptide."}
{"id": "PMID:1091479", "title": "Relationship of thyrotropin to exophthalmos-producing substance: formation of an exophthalmos-producing factor by pepsin digestion of mouse pituitary tumor and human thyrotropin preparations.", "content": "The present report demonstrates that mouse tumor thyrotropin preprarations have exophthalmogenic activity. In addition it shows that the exophthalmogenic activity of mouse tumor thyrotropin can be increased by partial pepsin digestion, whereas the thyroid stimulating activity of mouse tumor thyrotropin is rapidly destroyed by such treatment. Thus, after 30 min of pepsin digestion, mouse tumor thyrotropin has 130% of its initial exophthalmogenic action but only 10% of its thyroid stimulating activity. Preparations of human thyrotropin are similarly sensitive to partial pepsin digestion, i.e., there is a rapid destruction of thyroid stimulating activity but a very much slower destruction of exophthalmogenic activity. Thus, after 30 min of pepsin digestion, preparations of human thyrotropin retain 80% of their exophthalmogenic activity but only 20-30% of their thyroid stimulating action. Since these results are analogous to those obtained in studies of the partial pepsin digestion of bovine thyrotropin (1), partial pepsin digestion of both purified human and purified mouse tumor thyrotropin preparations should yield an exophthalmogenic fragment of the TSH molecule devoid of thyroid stimulating action.", "contents": "Relationship of thyrotropin to exophthalmos-producing substance: formation of an exophthalmos-producing factor by pepsin digestion of mouse pituitary tumor and human thyrotropin preparations. The present report demonstrates that mouse tumor thyrotropin preprarations have exophthalmogenic activity. In addition it shows that the exophthalmogenic activity of mouse tumor thyrotropin can be increased by partial pepsin digestion, whereas the thyroid stimulating activity of mouse tumor thyrotropin is rapidly destroyed by such treatment. Thus, after 30 min of pepsin digestion, mouse tumor thyrotropin has 130% of its initial exophthalmogenic action but only 10% of its thyroid stimulating activity. Preparations of human thyrotropin are similarly sensitive to partial pepsin digestion, i.e., there is a rapid destruction of thyroid stimulating activity but a very much slower destruction of exophthalmogenic activity. Thus, after 30 min of pepsin digestion, preparations of human thyrotropin retain 80% of their exophthalmogenic activity but only 20-30% of their thyroid stimulating action. Since these results are analogous to those obtained in studies of the partial pepsin digestion of bovine thyrotropin (1), partial pepsin digestion of both purified human and purified mouse tumor thyrotropin preparations should yield an exophthalmogenic fragment of the TSH molecule devoid of thyroid stimulating action."}
{"id": "PMID:1091480", "title": "Entomopathogens as insecticides.", "content": "Entomopathogens, diseases of insects, are suggested as a possible new generation of safe, selective insecticides. Over a thousand pathogens have been isolated from insects. Many of these, associated with major insect pests, are potential candidates for development into microbial insecticides. Phases in the development of a microbial insecticide are discussed as well as factors (production, safety, efficacy, registration, non-technical) which may influence this development.", "contents": "Entomopathogens as insecticides. Entomopathogens, diseases of insects, are suggested as a possible new generation of safe, selective insecticides. Over a thousand pathogens have been isolated from insects. Many of these, associated with major insect pests, are potential candidates for development into microbial insecticides. Phases in the development of a microbial insecticide are discussed as well as factors (production, safety, efficacy, registration, non-technical) which may influence this development."}
{"id": "PMID:1091481", "title": "Manipulating sex pheromones for insect suppression.", "content": "A review is given of different methods of using sex pheromones in pest control programs. Survey and monitoring traps have been used extensively for determining the presence and abundance of pest populations are commercially available. Pheromone trapping studies for insect suppression have shown success with some species and could be a useful tool in some situations. The technique of attracting insects to the host for insect removal or exposure to insecticide has also proved to be useful in some programs. Mating disruption studies using widely-separated pheromone evaporators or encapsulated pheromone distributed throughout an area have indicated a great potential for this technique in insect suppression programs.", "contents": "Manipulating sex pheromones for insect suppression. A review is given of different methods of using sex pheromones in pest control programs. Survey and monitoring traps have been used extensively for determining the presence and abundance of pest populations are commercially available. Pheromone trapping studies for insect suppression have shown success with some species and could be a useful tool in some situations. The technique of attracting insects to the host for insect removal or exposure to insecticide has also proved to be useful in some programs. Mating disruption studies using widely-separated pheromone evaporators or encapsulated pheromone distributed throughout an area have indicated a great potential for this technique in insect suppression programs."}
{"id": "PMID:1091482", "title": "Control of insects by sexual sterilization.", "content": "The control of insect populations by restricting their birth rate offers several advantages over methods based on increasing the death rate. The sterile-male technique scored practical success in the control of the screwworm, Cochliomyia hominivorax (Coquerel), but more general applications of the sterility procedure may be expected in conjunction with the development of integrated procedures for pest insect management.", "contents": "Control of insects by sexual sterilization. The control of insect populations by restricting their birth rate offers several advantages over methods based on increasing the death rate. The sterile-male technique scored practical success in the control of the screwworm, Cochliomyia hominivorax (Coquerel), but more general applications of the sterility procedure may be expected in conjunction with the development of integrated procedures for pest insect management."}
{"id": "PMID:1091483", "title": "Development of morphogenetic agents in insect control.", "content": "Chemicals which interfere with the growth and development of insects (morphogenetic agents) have been receiving major attention as potential means of selective insect control. Major advances in this field resulted from the identification of Juvenile Hormones -1, -2, and -3, and the discovery that various terpenoid and sesquiterpenoid derivatives were more potent morphogenetic agents than the three known Juvenile Hormones. Several highly active compounds have emerged from these research programs. Their field performance, problems, and prospects in selective insect control are considered here.", "contents": "Development of morphogenetic agents in insect control. Chemicals which interfere with the growth and development of insects (morphogenetic agents) have been receiving major attention as potential means of selective insect control. Major advances in this field resulted from the identification of Juvenile Hormones -1, -2, and -3, and the discovery that various terpenoid and sesquiterpenoid derivatives were more potent morphogenetic agents than the three known Juvenile Hormones. Several highly active compounds have emerged from these research programs. Their field performance, problems, and prospects in selective insect control are considered here."}
{"id": "PMID:1091484", "title": "Two-dimensional polyacrylamide-gel electrophoresis for purification of small RNAs specified by virulent coliphages T4, T5, T7 and BF23.", "content": "RNAs synthesized in Escherichia coli infected with virulent phages T4, T5, T7 and BF23 were labelled with 32PO4 3- after phage infection. [32P]RNAs of low molecular weight were separated by two-dimensional polyacrylamide gel electrophoresis, in which electrophoresis was carried out in two dimensions at different concentrations of acrylamide. The fractionated RNAs were characterized by RNA-fingerprint patterns made after T1 ribonuclease digestion. The two-dimensional gel of 10% yields 20% acrylamide was suitable for RNA of less than 200 nucleotides, while that of 5% yields 10% was preferred for RNAs of about 150--400 nucleotides. With T4 phage, 16 RNA species were separable on a single slab gel. Among those, 11 were identified as the known RNA species, including eight T4 tRNAs, one tRNA precursor and two non-tRNA molecules. In the case of T5 and BF23, more than 20 RNA species were separated on a slab gel; 15 or more RNAs were found in the 4-S RNA region, and several in 5-S and 6-S region. The RNA-fingerprint patterns of many BF23 RNAs were very similar to those of corresponding RNAs of T5. Pseudouridine and ribosylthymidine, minor nucleosides generally present in tRNA, were found in several BF23 4-S RNAs tested. Possibility of those BF23 4-S RNAs as tRNAs is discussed. With phage T7, three RNAs were detected, two of which were much smaller than tRNAs.", "contents": "Two-dimensional polyacrylamide-gel electrophoresis for purification of small RNAs specified by virulent coliphages T4, T5, T7 and BF23. RNAs synthesized in Escherichia coli infected with virulent phages T4, T5, T7 and BF23 were labelled with 32PO4 3- after phage infection. [32P]RNAs of low molecular weight were separated by two-dimensional polyacrylamide gel electrophoresis, in which electrophoresis was carried out in two dimensions at different concentrations of acrylamide. The fractionated RNAs were characterized by RNA-fingerprint patterns made after T1 ribonuclease digestion. The two-dimensional gel of 10% yields 20% acrylamide was suitable for RNA of less than 200 nucleotides, while that of 5% yields 10% was preferred for RNAs of about 150--400 nucleotides. With T4 phage, 16 RNA species were separable on a single slab gel. Among those, 11 were identified as the known RNA species, including eight T4 tRNAs, one tRNA precursor and two non-tRNA molecules. In the case of T5 and BF23, more than 20 RNA species were separated on a slab gel; 15 or more RNAs were found in the 4-S RNA region, and several in 5-S and 6-S region. The RNA-fingerprint patterns of many BF23 RNAs were very similar to those of corresponding RNAs of T5. Pseudouridine and ribosylthymidine, minor nucleosides generally present in tRNA, were found in several BF23 4-S RNAs tested. Possibility of those BF23 4-S RNAs as tRNAs is discussed. With phage T7, three RNAs were detected, two of which were much smaller than tRNAs."}
{"id": "PMID:1091485", "title": "Comparative studies on the properties of tryptophanase and tyrosine phenol-lyase immobilized directly on Sepharose or by use of Sepharose-bound pyridoxal 5'-phosphate.", "content": "Tryptophanase from Escherichia coli B/qt 7-A and tyrosine phenol-lyase (beta-tyrosinase) from Escherichia intermedia were immobilized on Sepharose 4B by several direct coupling reactions or through pyridoxal 5'-phosphate previously bound to Sepharose. The most active preparation of immobilized tryptophanase was obtained by coupling tetrameric apoenzyme to pyridoxal-P bound on Sepharose at the 6-position through a diazo linkage. This immobilization procedure involves the formation to Schiff base linkage between 4-formyl group of Sepharose-bound pyridoxal-P and the epsilon-amino group of the lysine residue at the active center of one subunit of tetrameric apo-tryptophanase, followed by the fixation of the Schiff base linkage by reduction with NaBH4. In the case of beta-tyrosinase having two catalytic centers, however, this method was not so suitable as the case of tryptophanase. Direct coupling of the apoenzyme to CNBr-activated Sepharose or to a bromoacetyl derivative of Sepharose gave better results. In each case, the affinity for substrate or coenzyme was scarcely influenced by the immobilization. When used repeatedly in a batch system or continuously in a flow system in the absence of added pyridoxal-P, immobilized holo-tryptophanase of holo-beta-tyrosinase gradually lost its original activity; however, supplement of pyridoxal-P to the reaction system restored its initial activity. From the kinetic analyses of these phenomena, the rate constants of coenzyme dissociation from immobilized tryptophanase and beta-tyrosinase were calculated. Upon immobilization, the pH optima of both enzymes shifted 0.5 to 1.0 pH unit to the alkaline side. Both immobilized enzymes showed higher thermal stability and resistance to a denaturing agent such as guinidine-HCl than their free counterpart. Furthermore, the reactivity of sulfhydryl group of beta-tyrosinase, in connection with its coenzyme-binding property, was conveniently studied by use of the immobilized enzyme.", "contents": "Comparative studies on the properties of tryptophanase and tyrosine phenol-lyase immobilized directly on Sepharose or by use of Sepharose-bound pyridoxal 5'-phosphate. Tryptophanase from Escherichia coli B/qt 7-A and tyrosine phenol-lyase (beta-tyrosinase) from Escherichia intermedia were immobilized on Sepharose 4B by several direct coupling reactions or through pyridoxal 5'-phosphate previously bound to Sepharose. The most active preparation of immobilized tryptophanase was obtained by coupling tetrameric apoenzyme to pyridoxal-P bound on Sepharose at the 6-position through a diazo linkage. This immobilization procedure involves the formation to Schiff base linkage between 4-formyl group of Sepharose-bound pyridoxal-P and the epsilon-amino group of the lysine residue at the active center of one subunit of tetrameric apo-tryptophanase, followed by the fixation of the Schiff base linkage by reduction with NaBH4. In the case of beta-tyrosinase having two catalytic centers, however, this method was not so suitable as the case of tryptophanase. Direct coupling of the apoenzyme to CNBr-activated Sepharose or to a bromoacetyl derivative of Sepharose gave better results. In each case, the affinity for substrate or coenzyme was scarcely influenced by the immobilization. When used repeatedly in a batch system or continuously in a flow system in the absence of added pyridoxal-P, immobilized holo-tryptophanase of holo-beta-tyrosinase gradually lost its original activity; however, supplement of pyridoxal-P to the reaction system restored its initial activity. From the kinetic analyses of these phenomena, the rate constants of coenzyme dissociation from immobilized tryptophanase and beta-tyrosinase were calculated. Upon immobilization, the pH optima of both enzymes shifted 0.5 to 1.0 pH unit to the alkaline side. Both immobilized enzymes showed higher thermal stability and resistance to a denaturing agent such as guinidine-HCl than their free counterpart. Furthermore, the reactivity of sulfhydryl group of beta-tyrosinase, in connection with its coenzyme-binding property, was conveniently studied by use of the immobilized enzyme."}
{"id": "PMID:1091486", "title": "An investigation of the 16-S RNA binding sites of ribosomal proteins S4, S8, S15, and S20 FROM Escherichia coli.", "content": "The RNA binding sites of four 30-S ribosomal subunit proteins from Escherichia coli, namely S4, S8, S15, and S20 were prepared from reconstituted single protein - 16-S-RNA complexes by mild enzymic digestion of non-protected RNA regions. Oligonucleotide fingerprints of the protected RNA regions were obtained and their positions were located within the 16-S-RNA sequence. They were not completely contiguous regions of RNA; oligonucleotides had been excised from each of them. The binding sites of S4 and S20, and those of S8 and S15 showed overlapping. The specificity of the RNA binding sites was confirmed by a reconstitution method.", "contents": "An investigation of the 16-S RNA binding sites of ribosomal proteins S4, S8, S15, and S20 FROM Escherichia coli. The RNA binding sites of four 30-S ribosomal subunit proteins from Escherichia coli, namely S4, S8, S15, and S20 were prepared from reconstituted single protein - 16-S-RNA complexes by mild enzymic digestion of non-protected RNA regions. Oligonucleotide fingerprints of the protected RNA regions were obtained and their positions were located within the 16-S-RNA sequence. They were not completely contiguous regions of RNA; oligonucleotides had been excised from each of them. The binding sites of S4 and S20, and those of S8 and S15 showed overlapping. The specificity of the RNA binding sites was confirmed by a reconstitution method."}
{"id": "PMID:1091487", "title": "The primary structure of the major cytoplasmic valine tRNA of mouse myeloma cells.", "content": "This paper describes the derivation of the primary structure of the major valine tRNA in the cytoplasm of mouse myeloma cells. Approximately 75% of the nucleotide sequence of this tRNA is also shared by the tRNA1-Val of yeast, this homology serving as a further indication of the extreme conservation of the structures of the tRNAs of different eukaryotic organisms. A novel feature of mouse myeloma tRNA1-Val is its loop IV sequence: -U-PSI-C-G-M1A-A-A-. This particular loop IV sequence has not previously been found in a tRNA structure. In addition, tRNA1-Val possesses some unusual nucleoside modifications. 5-Methyluridine (T) was not found to occur within loop IV of this tRNA, although this minor nucleoside is also absent from certain other mammalian tRNAs. Only one other tRNA, mammalian tRNAf-Met, has been found to possess 2-methylguanosine (m2G) in the position between the (b) and (c) stems of the cloverleaf. Numerous tRNAs have m2-2G in this location, and it would appear that the second methylation of this guanosine is characteristically absent from certain mammalian tRNA species.", "contents": "The primary structure of the major cytoplasmic valine tRNA of mouse myeloma cells. This paper describes the derivation of the primary structure of the major valine tRNA in the cytoplasm of mouse myeloma cells. Approximately 75% of the nucleotide sequence of this tRNA is also shared by the tRNA1-Val of yeast, this homology serving as a further indication of the extreme conservation of the structures of the tRNAs of different eukaryotic organisms. A novel feature of mouse myeloma tRNA1-Val is its loop IV sequence: -U-PSI-C-G-M1A-A-A-. This particular loop IV sequence has not previously been found in a tRNA structure. In addition, tRNA1-Val possesses some unusual nucleoside modifications. 5-Methyluridine (T) was not found to occur within loop IV of this tRNA, although this minor nucleoside is also absent from certain other mammalian tRNAs. Only one other tRNA, mammalian tRNAf-Met, has been found to possess 2-methylguanosine (m2G) in the position between the (b) and (c) stems of the cloverleaf. Numerous tRNAs have m2-2G in this location, and it would appear that the second methylation of this guanosine is characteristically absent from certain mammalian tRNA species."}
{"id": "PMID:1091518", "title": "The influence of histocompatibility and antithymocyte serum on the ability of alloantigenic pretreatment to prolong the survival of mouse skin grafts.", "content": "Test skin allografts enjoy prolonged survival when alloantigenic pretreatment (implants of small fragments of liver or kidney on the host's kidney cortex) is separated from test grafting by 9 days and donor and host strains differ only at the relatively weak H-3 + H-13 histocompatibility barrier (Baldwin and Cohen 1971, 1974). However, test skin grafts are rejected in an accelerated fashion when this protocol is followed but the donor strains differ at stronger non-H-2 barriers. In 2 such strain combinations in which control first-set grafts are rejected acutely, varying the implant-to-test graft interval, using F-1 mice as implant donors, or varying the sex of the donors and hosts, all failed to prolong test graft SURVIVAL. However, survival of some test grafts was significantly greater following a combined host treatment of alloimplanatation and ATS than it was following either procedure alone. This synergism suggests that across stronger H-barriers, alloantigenic pretreatment does elicit an immunosuppressive component whose effective presence is normally masked by thymus-dependent cell-mediated immunity.", "contents": "The influence of histocompatibility and antithymocyte serum on the ability of alloantigenic pretreatment to prolong the survival of mouse skin grafts. Test skin allografts enjoy prolonged survival when alloantigenic pretreatment (implants of small fragments of liver or kidney on the host's kidney cortex) is separated from test grafting by 9 days and donor and host strains differ only at the relatively weak H-3 + H-13 histocompatibility barrier (Baldwin and Cohen 1971, 1974). However, test skin grafts are rejected in an accelerated fashion when this protocol is followed but the donor strains differ at stronger non-H-2 barriers. In 2 such strain combinations in which control first-set grafts are rejected acutely, varying the implant-to-test graft interval, using F-1 mice as implant donors, or varying the sex of the donors and hosts, all failed to prolong test graft SURVIVAL. However, survival of some test grafts was significantly greater following a combined host treatment of alloimplanatation and ATS than it was following either procedure alone. This synergism suggests that across stronger H-barriers, alloantigenic pretreatment does elicit an immunosuppressive component whose effective presence is normally masked by thymus-dependent cell-mediated immunity."}
{"id": "PMID:1091524", "title": "[The ideal computer for ECG analysis (author's transl)].", "content": "An on-line minicomputer with a suitable structure turns out to be the ideal system for ECG interpretation. The motivations for this conclusion come both from the nature of the ECG analysis process and from the solution that the present state of the process and from the solution that the present state of the computer technology suggests for the controversy on large-scale computers versus minicomputers in the medical environment. In this paper both these aspects of the problem are briefly discussed and an ideal structure is proposed for a system based on a minicomputer. The main goals of this system are flexibility, adequate man-machine interfacing, low cost, high reliability and minimal maintenance, minimum installation space and site preparation.", "contents": "[The ideal computer for ECG analysis (author's transl)]. An on-line minicomputer with a suitable structure turns out to be the ideal system for ECG interpretation. The motivations for this conclusion come both from the nature of the ECG analysis process and from the solution that the present state of the process and from the solution that the present state of the computer technology suggests for the controversy on large-scale computers versus minicomputers in the medical environment. In this paper both these aspects of the problem are briefly discussed and an ideal structure is proposed for a system based on a minicomputer. The main goals of this system are flexibility, adequate man-machine interfacing, low cost, high reliability and minimal maintenance, minimum installation space and site preparation."}
{"id": "PMID:1091527", "title": "Research approaches in illicit drug use: a critical review.", "content": "Research approached and substantive findings pertaining to determinants of the nonmedical use of illicit psychoactive drugs are reviewed. Discussion focuses upon the dimensions of drug use as a behaviorial phenomenon, the findings and limitations of four basic approaches to research in the ares--sociocultural, psychopathological, functionalist, and social learning-- and upon methodological problems. Drug use must be treated as a multidimensional phenomenon, with analyses extending well beyond simple user vs. nonuser comparisons or arbitrary categories of users. Research must be conducted within a coherent field-theoretical conceptual framework, directed toward the formulation of nomothetic laws concerning patterns of drug use. Inadequacies in sampling and measurement and the absence of longitudinal design have compromised the utility of a vast research literature.", "contents": "Research approaches in illicit drug use: a critical review. Research approached and substantive findings pertaining to determinants of the nonmedical use of illicit psychoactive drugs are reviewed. Discussion focuses upon the dimensions of drug use as a behaviorial phenomenon, the findings and limitations of four basic approaches to research in the ares--sociocultural, psychopathological, functionalist, and social learning-- and upon methodological problems. Drug use must be treated as a multidimensional phenomenon, with analyses extending well beyond simple user vs. nonuser comparisons or arbitrary categories of users. Research must be conducted within a coherent field-theoretical conceptual framework, directed toward the formulation of nomothetic laws concerning patterns of drug use. Inadequacies in sampling and measurement and the absence of longitudinal design have compromised the utility of a vast research literature."}
{"id": "PMID:1091532", "title": "The effect of wheat bran on intestinal transit.", "content": "In 18 students and two members of staff at a boys' boarding school, the time taken to pass 20 out of 25 radiopaque pellets varied from one to seven days while the subjects were eating a normal English diet. After the additon of bran, about 20 g daily, to this diet transit time fell from 2-75 plus or minus 1-6 to 2-0 plus or minus 0-9 days (P smaller than 0-025). Transit became faster in all nine subjects who had an initial time of three days or more, and in three of seven with an initial time of two days, but became slower in all four boys with an initial one-day transit. Frequency of defaecation correlated poorly with transit time (a once daily bowel action being found with transit times ranging from one to four days), and did not increase significantly with bran. In 10 additional adults with slow initial transit (three or four days) the effect of bran was compared with that of an equal volume of ground oatflakes in a double-blind crossover trial. Bran caused a significant acceleration of transit, wherease oatmeal had no effect. These studies confirm that bran accelerates slow intestinal transit and show that this is not simply a psychological effect. Bran may also slow down fast transit.", "contents": "The effect of wheat bran on intestinal transit. In 18 students and two members of staff at a boys' boarding school, the time taken to pass 20 out of 25 radiopaque pellets varied from one to seven days while the subjects were eating a normal English diet. After the additon of bran, about 20 g daily, to this diet transit time fell from 2-75 plus or minus 1-6 to 2-0 plus or minus 0-9 days (P smaller than 0-025). Transit became faster in all nine subjects who had an initial time of three days or more, and in three of seven with an initial time of two days, but became slower in all four boys with an initial one-day transit. Frequency of defaecation correlated poorly with transit time (a once daily bowel action being found with transit times ranging from one to four days), and did not increase significantly with bran. In 10 additional adults with slow initial transit (three or four days) the effect of bran was compared with that of an equal volume of ground oatflakes in a double-blind crossover trial. Bran caused a significant acceleration of transit, wherease oatmeal had no effect. These studies confirm that bran accelerates slow intestinal transit and show that this is not simply a psychological effect. Bran may also slow down fast transit."}
{"id": "PMID:1091533", "title": "Complete identification of endocrine cells in the gastrointestinal tract using semithin-thin sections to identify motilin cells in human and animal intestine.", "content": "The present (Wiesbaden) classification of gut endocrine cells relies mainly on ultrastructure and the results of silver impregnation. Correlation with hormone production requires parallel immunology but conventional immune cytochemistry is, in most cases, difficult or at present impossible. The serial, semithin-thin-section technique offers an alternative, which provides absolute correlation between cell type and hormone production. The successful use of the technique is illustrated here by localization of motilin in the enterochromaffin cells of the small intestine.", "contents": "Complete identification of endocrine cells in the gastrointestinal tract using semithin-thin sections to identify motilin cells in human and animal intestine. The present (Wiesbaden) classification of gut endocrine cells relies mainly on ultrastructure and the results of silver impregnation. Correlation with hormone production requires parallel immunology but conventional immune cytochemistry is, in most cases, difficult or at present impossible. The serial, semithin-thin-section technique offers an alternative, which provides absolute correlation between cell type and hormone production. The successful use of the technique is illustrated here by localization of motilin in the enterochromaffin cells of the small intestine."}
{"id": "PMID:1091543", "title": "Separation of T lymphocytes from normal individuals and patients with B lymphocyte chronic lymphocytic leukaemia.", "content": "Previous studies have shown that lymphocytes from patients with chronic lymphocytic leukaemia have a diminished response to mitogens which stimulate T cells. Chronic lymphocytic leukaemia is most often a disease of accumulating B cells so that T lymphocytes are diluted by large numbers of leukaemic cells. Direct comparison with the responses of normal lymphocytes to mitogenic stimulation is therefore suspect. To circumvent this difficulty, a method of isolating T cells from normal individuals and patients with chronic lymphocytic leukaemia was developed. Lymphocytes containing an average of 16.1 per cent B cells from normal individuals were applied to IgG-anti-IgG-coated Degalan bead columns and held at 4 degrees for 2 hours. The eluted cells contained less than 2 per cent B cells. When chronic lymphocytic leukaemic lymphocytes, containing an average of 68.6 per cent B cells, were applied to IgG-anti-IgG columns, the eluted cells contained 36.4 per cent B cells. To improve the purification of T lymphocytes, columns of uncoated Degalan beads were used to remove non-specifically adherent cells. Eluted lymphocytes were then applied to IgG-anti-IgG columns. This resulted in the recovery of purified populations of T cells with less than 2 per cent contamination with B cells. Patients with chronic lymphocytic leukaemia were found to have lymphocytes with either a normal density or a low density of surface immunoglobulins. B cells were successfully removed from lymphocyte suspensions in all cases of chronic lymphocytic leukaemia with a normal density of lymphocyte surface immunoglobulins. In the three cases of chronic lymphocytic leukaemia with low density surface immunoglobulins, separation by this method was unsuccessful. However, an enriched T-cell population was obtained when leukaemic lymphocytes which had lost all detectable surface immunoglobulins were passed through a column coated with heat-aggregated IgG.", "contents": "Separation of T lymphocytes from normal individuals and patients with B lymphocyte chronic lymphocytic leukaemia. Previous studies have shown that lymphocytes from patients with chronic lymphocytic leukaemia have a diminished response to mitogens which stimulate T cells. Chronic lymphocytic leukaemia is most often a disease of accumulating B cells so that T lymphocytes are diluted by large numbers of leukaemic cells. Direct comparison with the responses of normal lymphocytes to mitogenic stimulation is therefore suspect. To circumvent this difficulty, a method of isolating T cells from normal individuals and patients with chronic lymphocytic leukaemia was developed. Lymphocytes containing an average of 16.1 per cent B cells from normal individuals were applied to IgG-anti-IgG-coated Degalan bead columns and held at 4 degrees for 2 hours. The eluted cells contained less than 2 per cent B cells. When chronic lymphocytic leukaemic lymphocytes, containing an average of 68.6 per cent B cells, were applied to IgG-anti-IgG columns, the eluted cells contained 36.4 per cent B cells. To improve the purification of T lymphocytes, columns of uncoated Degalan beads were used to remove non-specifically adherent cells. Eluted lymphocytes were then applied to IgG-anti-IgG columns. This resulted in the recovery of purified populations of T cells with less than 2 per cent contamination with B cells. Patients with chronic lymphocytic leukaemia were found to have lymphocytes with either a normal density or a low density of surface immunoglobulins. B cells were successfully removed from lymphocyte suspensions in all cases of chronic lymphocytic leukaemia with a normal density of lymphocyte surface immunoglobulins. In the three cases of chronic lymphocytic leukaemia with low density surface immunoglobulins, separation by this method was unsuccessful. However, an enriched T-cell population was obtained when leukaemic lymphocytes which had lost all detectable surface immunoglobulins were passed through a column coated with heat-aggregated IgG."}
{"id": "PMID:1091544", "title": "Thymus dependence of the IgA response to sheep erythrocytes.", "content": "The thumus dependence of the IgA response to sheep red blooc cells (SRBC) was studied by means of cell transfer experiments in mice. Only low numbers of IgM-, IgG- and IgA-plaque-forming cells (PFC) were observed in those recipients which received only spleen cells from adult thymectomized, irradiated and bone marrow-reconstituted mice (B cells). High numbers of IgM-, IgG- and IgA-PFC were observed when B cells and educated T cells were transferred to the recipients. Evidence is provided that B cells committed to IgA synthesis require the same degree of interaction with T cells as B cells committed to IgM synthesis, but a lower degree of interaction with T cells than B cells committed to IgG synthesis.", "contents": "Thymus dependence of the IgA response to sheep erythrocytes. The thumus dependence of the IgA response to sheep red blooc cells (SRBC) was studied by means of cell transfer experiments in mice. Only low numbers of IgM-, IgG- and IgA-plaque-forming cells (PFC) were observed in those recipients which received only spleen cells from adult thymectomized, irradiated and bone marrow-reconstituted mice (B cells). High numbers of IgM-, IgG- and IgA-PFC were observed when B cells and educated T cells were transferred to the recipients. Evidence is provided that B cells committed to IgA synthesis require the same degree of interaction with T cells as B cells committed to IgM synthesis, but a lower degree of interaction with T cells than B cells committed to IgG synthesis."}
{"id": "PMID:1091545", "title": "The possible presence of a bursa-independent, IgM-producing system in chicks.", "content": "The number of splenic plaque-forming cells significantly increased in bursectomized chicks immunized secondarily with SRBC. Further experiments using rabbit antiserum specific to the bursa of Fabricius indicate that the different types of antibody-producing cells might be engaged in the synthesis of IgM in bursectomized chicks. The possible presence of a bursa-independent, IgM-producing system in chicks is suggested.", "contents": "The possible presence of a bursa-independent, IgM-producing system in chicks. The number of splenic plaque-forming cells significantly increased in bursectomized chicks immunized secondarily with SRBC. Further experiments using rabbit antiserum specific to the bursa of Fabricius indicate that the different types of antibody-producing cells might be engaged in the synthesis of IgM in bursectomized chicks. The possible presence of a bursa-independent, IgM-producing system in chicks is suggested."}
{"id": "PMID:1091546", "title": "Growth of several cariogenic strains of oral streptococci in a chemically defined medium.", "content": "A chemically defined medium in which Streptococcus mutans strains AHT, BHT, GS-5, JC-2, Ingbritt, At6T, At9T, 6715, and OMZ-176 and Streptococcus salivarius strain HHT grew rapidly to high turbidities was formulated. Maximal turbidities of each strain were observed after 8 to 12 h of aerobic growth. The subsequent transfer of exponentially growing cells into fresh medium resulted in growth at the same rate without lag. Growth of these strains occurred with rates at least one-half of those observed in an organic medium, such as Todd-Hewitt broth. S. mutans strains FA-1 and OMZ-61 grew at relatively slow rates in the defined medium, but more rapidly growth to higher turbidities of both strains was obtained when sodium bicarbonate was added to the medium. Streptococcus sanguis strain OMZ-9 and another group H streptococcus (strain 72 times 46) grew rapidly in the defined medium after the addition of sodium carbonate. The presence of carbonate or bicarbonate yielded higher turbidities of all the other strains, and the growth rates of several of the strains tested were also increased.", "contents": "Growth of several cariogenic strains of oral streptococci in a chemically defined medium. A chemically defined medium in which Streptococcus mutans strains AHT, BHT, GS-5, JC-2, Ingbritt, At6T, At9T, 6715, and OMZ-176 and Streptococcus salivarius strain HHT grew rapidly to high turbidities was formulated. Maximal turbidities of each strain were observed after 8 to 12 h of aerobic growth. The subsequent transfer of exponentially growing cells into fresh medium resulted in growth at the same rate without lag. Growth of these strains occurred with rates at least one-half of those observed in an organic medium, such as Todd-Hewitt broth. S. mutans strains FA-1 and OMZ-61 grew at relatively slow rates in the defined medium, but more rapidly growth to higher turbidities of both strains was obtained when sodium bicarbonate was added to the medium. Streptococcus sanguis strain OMZ-9 and another group H streptococcus (strain 72 times 46) grew rapidly in the defined medium after the addition of sodium carbonate. The presence of carbonate or bicarbonate yielded higher turbidities of all the other strains, and the growth rates of several of the strains tested were also increased."}
{"id": "PMID:1091547", "title": "Amino acid requirements of Streptococcus mutans and other oral streptococci.", "content": "The amino acid requirements of Streptococcus mutans strains AHT, OMZ-61, FA-1, BHT, GS-5, JC-2, Ingbritt, At6T, OMZ-176, 6715, Streptococcus salivarius HHT, Streptococcus sanguis OMZ-9, and strain 72x46 were determined in a chemically defined medium. When grown anaerobically in the presence of sodium carbonate (or bicarbonate for a few strains), few amino acids were required. All strains tested required cystine (or cystine) as a nutrient. Three strains (S. mutans OMZ-176, FA-1, and BHT) required glutamate (and/or glutamine). A third amino acid (lysine for S. mutans FA-1 and histidine for S. mutans OMZ-176) was required by two of the three strains which required glutamate. The amino acids mentioned above were required for all conditions of incubation (and inoculum) tested. The requirements for several other amino acids were conditional, that is, dependent on the incubation conditions and inoculum used. For example, when carbonate was not added, glutamate was required by S. mutans GS-5. Aerobic incubations, with carbonate or bicarbonate added, resluted in requirements for glutamate and leucine by several strains. With these incubation conditions, one strain required isoleucine (S. mutans FA-1), another valine (S. mutans AHT), and a third tyrosine (72x46). Aerobic incubations in the absence of carbonate or bicarbonate further increased the number of amino acids required by several strains. Furthermore, when stationary-phase cultures replaced exponentially growing cultures as an inoculum, several strains required additional amino acids, presumably for the initiation of growth.", "contents": "Amino acid requirements of Streptococcus mutans and other oral streptococci. The amino acid requirements of Streptococcus mutans strains AHT, OMZ-61, FA-1, BHT, GS-5, JC-2, Ingbritt, At6T, OMZ-176, 6715, Streptococcus salivarius HHT, Streptococcus sanguis OMZ-9, and strain 72x46 were determined in a chemically defined medium. When grown anaerobically in the presence of sodium carbonate (or bicarbonate for a few strains), few amino acids were required. All strains tested required cystine (or cystine) as a nutrient. Three strains (S. mutans OMZ-176, FA-1, and BHT) required glutamate (and/or glutamine). A third amino acid (lysine for S. mutans FA-1 and histidine for S. mutans OMZ-176) was required by two of the three strains which required glutamate. The amino acids mentioned above were required for all conditions of incubation (and inoculum) tested. The requirements for several other amino acids were conditional, that is, dependent on the incubation conditions and inoculum used. For example, when carbonate was not added, glutamate was required by S. mutans GS-5. Aerobic incubations, with carbonate or bicarbonate added, resluted in requirements for glutamate and leucine by several strains. With these incubation conditions, one strain required isoleucine (S. mutans FA-1), another valine (S. mutans AHT), and a third tyrosine (72x46). Aerobic incubations in the absence of carbonate or bicarbonate further increased the number of amino acids required by several strains. Furthermore, when stationary-phase cultures replaced exponentially growing cultures as an inoculum, several strains required additional amino acids, presumably for the initiation of growth."}
{"id": "PMID:1091548", "title": "Phage conversion of Shigella flexneri group antigens.", "content": "A temperate phage, designated Sf6, has been isolated from Shigella flexneri 3a. Characterization of Sf6 revealed that it possesses the capacity for converting the S. flexneri 3,4 group antigen complex to group factor 6. Serological studies and chemical analysis of lipopolysaccharide from converted strains suggest that group factor 6 is a reflection of an acetylation of the preexisting 3,4 antigen complex. Evidence is provided that the 3,4 group antigen complex functions, at least in part, as a cell surface receptor site for Sf6 adsorption.", "contents": "Phage conversion of Shigella flexneri group antigens. A temperate phage, designated Sf6, has been isolated from Shigella flexneri 3a. Characterization of Sf6 revealed that it possesses the capacity for converting the S. flexneri 3,4 group antigen complex to group factor 6. Serological studies and chemical analysis of lipopolysaccharide from converted strains suggest that group factor 6 is a reflection of an acetylation of the preexisting 3,4 antigen complex. Evidence is provided that the 3,4 group antigen complex functions, at least in part, as a cell surface receptor site for Sf6 adsorption."}
{"id": "PMID:1091549", "title": "Neutralization of Chlamydia trachomatis in cell culture.", "content": "Neutralization of Chlamydia trachomatis was assayed by the decrease in inclusion-forming units in baby hamster kidney cells grown in culture. Five percent fresh guinea pig sera increased neutralization titers of rabbit antisera 100- to 1,000-fold but had no effect when normal rabbit sera were tested. Neutralization of a type A or B trachoma isolate was strain specific. Neutralization by human eye secretions and sera also was demonstrated when guinea pig sera were included in the test. All of the six human sera tested showed strain specificity against types A or B, in agreement with typing by the fluorescent antibody technique.", "contents": "Neutralization of Chlamydia trachomatis in cell culture. Neutralization of Chlamydia trachomatis was assayed by the decrease in inclusion-forming units in baby hamster kidney cells grown in culture. Five percent fresh guinea pig sera increased neutralization titers of rabbit antisera 100- to 1,000-fold but had no effect when normal rabbit sera were tested. Neutralization of a type A or B trachoma isolate was strain specific. Neutralization by human eye secretions and sera also was demonstrated when guinea pig sera were included in the test. All of the six human sera tested showed strain specificity against types A or B, in agreement with typing by the fluorescent antibody technique."}
{"id": "PMID:1091550", "title": "Predominant cultivable flora isolated from human root surface caries plaque.", "content": "Plaque samples were obtained from tooth surfaces exhibiting typical lesions of root surface caries and were immediately cultured by a continuous anaerobic procedure. The bacterial composition of root caries flora was determined on individual samples. Representative isolates from each specimen were characterized by morphological and physiological criteria. In addition, fluorescent antibody reagents were used to confirm the identification of Streptococcus mutans and Actinomyces viscosus. The plaque samples could be divided into two groups on the basis of the presence or absence of S. mutans in the plaque. In group I plaques, S. mutans comprised 30 percent of the total cultivable flora. S. sanguis was either not found or was present in very low number. In group II plaques, S. mutans was not detected, and S. sanguis formed 48 percent of the total plaque flora. A. viscosus was the dominant organism in all plaque samples, accounting for 47 percent of the group I isolates and 41 percent of the group II isolates.", "contents": "Predominant cultivable flora isolated from human root surface caries plaque. Plaque samples were obtained from tooth surfaces exhibiting typical lesions of root surface caries and were immediately cultured by a continuous anaerobic procedure. The bacterial composition of root caries flora was determined on individual samples. Representative isolates from each specimen were characterized by morphological and physiological criteria. In addition, fluorescent antibody reagents were used to confirm the identification of Streptococcus mutans and Actinomyces viscosus. The plaque samples could be divided into two groups on the basis of the presence or absence of S. mutans in the plaque. In group I plaques, S. mutans comprised 30 percent of the total cultivable flora. S. sanguis was either not found or was present in very low number. In group II plaques, S. mutans was not detected, and S. sanguis formed 48 percent of the total plaque flora. A. viscosus was the dominant organism in all plaque samples, accounting for 47 percent of the group I isolates and 41 percent of the group II isolates."}
{"id": "PMID:1091551", "title": "Evidence for the non-protein nature of the receptor for the enterotoxin in Vibrio cholerae on murine lymphoid cells.", "content": "Lymphoid cells from A/J and BALB/c strains of mice were iodinated with 125I by the lactoperoxidase method and the plasma membranes were disrupted by freezing and thawing or with 0.5 percent Nonidet P-40, a nonionic detergent. Attempts to find choleragen reactive iodinated material in 0.5 percent Nonidet P-40 lysates were unsuccessful even when the cells were incubated with choleragen before lysis. Freezing and thawing the cells resulted in the release of iodinated choleragen reactive material. The interaction of choleragen with the iodinated material could be inhibited (at low choleragen concentrations) or enhanced (at high choleragen concentrations) by the addition of the ganglioside G-M1 to the the immune precipitation system. The results are consistent with the hypothesis that the receptor for choleragen is a glycolipid and reduce, but do not totally eliminate, the likelihood that the receptor is glycoprotein in nature.", "contents": "Evidence for the non-protein nature of the receptor for the enterotoxin in Vibrio cholerae on murine lymphoid cells. Lymphoid cells from A/J and BALB/c strains of mice were iodinated with 125I by the lactoperoxidase method and the plasma membranes were disrupted by freezing and thawing or with 0.5 percent Nonidet P-40, a nonionic detergent. Attempts to find choleragen reactive iodinated material in 0.5 percent Nonidet P-40 lysates were unsuccessful even when the cells were incubated with choleragen before lysis. Freezing and thawing the cells resulted in the release of iodinated choleragen reactive material. The interaction of choleragen with the iodinated material could be inhibited (at low choleragen concentrations) or enhanced (at high choleragen concentrations) by the addition of the ganglioside G-M1 to the the immune precipitation system. The results are consistent with the hypothesis that the receptor for choleragen is a glycolipid and reduce, but do not totally eliminate, the likelihood that the receptor is glycoprotein in nature."}
{"id": "PMID:1091552", "title": "Effects of human and rabbit serum on viability, permeability, and envelope lipids of Serratia marcescens.", "content": "The major action of serum on gram-negative organisms is thought to be on the microbial envelope. We compared the effects of normal human and rabbit serum on the envelope lipids of two strains of Serratia marcescens, one sensitive and one resistant to the bactericidal effects of serum. During killing by either serum, the sensitive strain underwent rapid permeability changes coincident with degradation of microbial phospholipids. The resistant strain exhibited none of these effects. The phospholipid degradation that accompanies killing of the sensitive strain by serum could be caused by phospholipases present in serum or by Serratia's own phospholipid-splitting enzymes. The results indicate that phospholipid breakdown is caused by activation of bacterial of bacterial phospholipases and not by serum phospholipases. This conclusion is based upon the following findings.(i1 Although rabbit serum phospholipase A was at least 10 times more active than human serum phospholipase A, phospholipid degradation in the sensitive Serratia strain was comparable during (equally rapid) killing by human or rabbit serum. (ii) Heat treatment (56 C) of both sera eliminated bactericidal activity as well as microbial lipid degradation but abolished phospholipase activity of human serum only. (iii) Virtually complete removal of phospholipase A activity from human serum by adsorption onto autoclaved Micrococcus lysodeikticus had no effect on the extent of phospholipid hydrolysis or on bactericidal activity. Activation by serum of endogenous phospholipase activity in S. marcescens was accompanied by enhanced incorporation of lipid precursors into bacterial lipids. No evidence was found for increased turnover of protein or ribonucleic acid during killing by serum.", "contents": "Effects of human and rabbit serum on viability, permeability, and envelope lipids of Serratia marcescens. The major action of serum on gram-negative organisms is thought to be on the microbial envelope. We compared the effects of normal human and rabbit serum on the envelope lipids of two strains of Serratia marcescens, one sensitive and one resistant to the bactericidal effects of serum. During killing by either serum, the sensitive strain underwent rapid permeability changes coincident with degradation of microbial phospholipids. The resistant strain exhibited none of these effects. The phospholipid degradation that accompanies killing of the sensitive strain by serum could be caused by phospholipases present in serum or by Serratia's own phospholipid-splitting enzymes. The results indicate that phospholipid breakdown is caused by activation of bacterial of bacterial phospholipases and not by serum phospholipases. This conclusion is based upon the following findings.(i1 Although rabbit serum phospholipase A was at least 10 times more active than human serum phospholipase A, phospholipid degradation in the sensitive Serratia strain was comparable during (equally rapid) killing by human or rabbit serum. (ii) Heat treatment (56 C) of both sera eliminated bactericidal activity as well as microbial lipid degradation but abolished phospholipase activity of human serum only. (iii) Virtually complete removal of phospholipase A activity from human serum by adsorption onto autoclaved Micrococcus lysodeikticus had no effect on the extent of phospholipid hydrolysis or on bactericidal activity. Activation by serum of endogenous phospholipase activity in S. marcescens was accompanied by enhanced incorporation of lipid precursors into bacterial lipids. No evidence was found for increased turnover of protein or ribonucleic acid during killing by serum."}
{"id": "PMID:1091553", "title": "Persistence of rubellavirus-specific immunoglobulin M and immunoglobulin A antibodies: investigation of successive serum samples with lowered immunoglobulin G concentration.", "content": "The persistence of rubellavirus-specific immunoglobulin (Ig) M and IgA antibodies has been studied in seven patients with primary postnatal rubella infections. Successive blood samples obtained over a period of several years after the onset of disease have been investigated, employing the fluorescent antibody technique and the sucrose gradient centrifugation method. IgM antibodies were found to persist for 4 to 5 weeks after the onset of disease, with only moderate variation being observed with respect to the different patients and the method of investigation being studied. The persistence of IgA antibodies (as examined by the fluorescent antibody technique) varied from a few weeks to several years after the onset of the disease. The detection of IgA antibodies cannot be considered as conclusive for the diagnosis of recent rubella infections.", "contents": "Persistence of rubellavirus-specific immunoglobulin M and immunoglobulin A antibodies: investigation of successive serum samples with lowered immunoglobulin G concentration. The persistence of rubellavirus-specific immunoglobulin (Ig) M and IgA antibodies has been studied in seven patients with primary postnatal rubella infections. Successive blood samples obtained over a period of several years after the onset of disease have been investigated, employing the fluorescent antibody technique and the sucrose gradient centrifugation method. IgM antibodies were found to persist for 4 to 5 weeks after the onset of disease, with only moderate variation being observed with respect to the different patients and the method of investigation being studied. The persistence of IgA antibodies (as examined by the fluorescent antibody technique) varied from a few weeks to several years after the onset of the disease. The detection of IgA antibodies cannot be considered as conclusive for the diagnosis of recent rubella infections."}
{"id": "PMID:1091554", "title": "Virologic and serologic studies of zoo birds for Marek's disease virus infection.", "content": "One hundred and eleven zoo birds representing 49 species in 14 orders were examined for Marek's disease (MD) herpesvirus (MDHV) infection. MDHV was isolated from 10 birds, all belonging to genus Gallus. The precipitating antibodies against MDHV were demonstrated only in the Gallus birds, when 51 selected birds including 34 Galliformes and 17 other birds representing 12 species from nine orders were examined. The 10 MDHV isolates all induced morphologically similar plaques in cell cultures closely resembling those of HN strain, a low pathogenic isolate of MDHV. Six of the 10 isolates, when inoculated into an experimental line of chickens highly susceptible to MD, caused only a minimal degree of histologic lesions without causing clinical MD, gross MD lesions, or deaths from MD. Natural hosts of MD are probably Galliformes, primarily affecting Gallus and less often other genera of Galliformes.", "contents": "Virologic and serologic studies of zoo birds for Marek's disease virus infection. One hundred and eleven zoo birds representing 49 species in 14 orders were examined for Marek's disease (MD) herpesvirus (MDHV) infection. MDHV was isolated from 10 birds, all belonging to genus Gallus. The precipitating antibodies against MDHV were demonstrated only in the Gallus birds, when 51 selected birds including 34 Galliformes and 17 other birds representing 12 species from nine orders were examined. The 10 MDHV isolates all induced morphologically similar plaques in cell cultures closely resembling those of HN strain, a low pathogenic isolate of MDHV. Six of the 10 isolates, when inoculated into an experimental line of chickens highly susceptible to MD, caused only a minimal degree of histologic lesions without causing clinical MD, gross MD lesions, or deaths from MD. Natural hosts of MD are probably Galliformes, primarily affecting Gallus and less often other genera of Galliformes."}
{"id": "PMID:1091555", "title": "Detection of a leukocytic endogenous mediator-like mediator of serum amino acid and zinc depression during various infectious illnesses.", "content": "Serum samples from patients with many different kinds of infection were shown to contain a substance characteristic of leukocytic endogenous mediator. Generalized bacterial infections produced more leukocytic endogenous mediator than did localized ones; viral infections produced very little. In typhoid fever, mediator concentrations increased before the onset of illness.", "contents": "Detection of a leukocytic endogenous mediator-like mediator of serum amino acid and zinc depression during various infectious illnesses. Serum samples from patients with many different kinds of infection were shown to contain a substance characteristic of leukocytic endogenous mediator. Generalized bacterial infections produced more leukocytic endogenous mediator than did localized ones; viral infections produced very little. In typhoid fever, mediator concentrations increased before the onset of illness."}
{"id": "PMID:1091556", "title": "Significane of intravascular coagulation in canine endotoxin shock.", "content": "The contribution of disseminated fibrin clot formation to the pathogenesis of canine endotoxin shock was explored in control dogs and in those defibrinated with a purified fraction of Malayan pit viper venom. The hemodynamic and humoral responses after the administration of an intravenous challenge dose of Escherichia coli endotoxin were comparable as was mortality. It is concluded that, although the role of the coagulation sequence in canine endotoxin shock is unclear, it does not appear to be determinative.", "contents": "Significane of intravascular coagulation in canine endotoxin shock. The contribution of disseminated fibrin clot formation to the pathogenesis of canine endotoxin shock was explored in control dogs and in those defibrinated with a purified fraction of Malayan pit viper venom. The hemodynamic and humoral responses after the administration of an intravenous challenge dose of Escherichia coli endotoxin were comparable as was mortality. It is concluded that, although the role of the coagulation sequence in canine endotoxin shock is unclear, it does not appear to be determinative."}
{"id": "PMID:1091557", "title": "Factors affecting filamentation in Candida albicans: relationship of the uptake and distribution of proline to morphogenesis.", "content": "When glucose was present in high concentration, Candida albicans formed filaments in a phosphate-buffered medium, regardless of the nitrogen source. In lower concentrations of glucose, filamentation occurred only when various members of the glutamate, succinyl, or acetoacetyl-coenzyme A families of amino acids were used as sole nitrogen sources. Yeast morphology could be maintained either by replacing the amino acids in the medium with ammonium chloride or by making the medium high in phosphate or biotin. Studies using [U-14C]proline indicated that proline was catabolized in a manner consistent with the generation of increased cellular reducing potential and that the proline label entered into the Kreb's cycle. A reduction in Kreb's cycle activity was evidenced by an initial increase and then a rapid drop of the total organic acid content of the cells as well as in specific Kreb's cycle intermediates. Filamentation under conditions of low phosphate, high glucose, and increased cellular reduction potential, accompanied by a decrease in Kreb's cycle activity, suggests that morphogenesis in C. albicans is correlated with a Crabtree-like effect, i.e., repression of mitochondrial activity.", "contents": "Factors affecting filamentation in Candida albicans: relationship of the uptake and distribution of proline to morphogenesis. When glucose was present in high concentration, Candida albicans formed filaments in a phosphate-buffered medium, regardless of the nitrogen source. In lower concentrations of glucose, filamentation occurred only when various members of the glutamate, succinyl, or acetoacetyl-coenzyme A families of amino acids were used as sole nitrogen sources. Yeast morphology could be maintained either by replacing the amino acids in the medium with ammonium chloride or by making the medium high in phosphate or biotin. Studies using [U-14C]proline indicated that proline was catabolized in a manner consistent with the generation of increased cellular reducing potential and that the proline label entered into the Kreb's cycle. A reduction in Kreb's cycle activity was evidenced by an initial increase and then a rapid drop of the total organic acid content of the cells as well as in specific Kreb's cycle intermediates. Filamentation under conditions of low phosphate, high glucose, and increased cellular reduction potential, accompanied by a decrease in Kreb's cycle activity, suggests that morphogenesis in C. albicans is correlated with a Crabtree-like effect, i.e., repression of mitochondrial activity."}
{"id": "PMID:1091558", "title": "Immunodepression by Rowson-Parr virus in mice: effect of Rowson-Parr virus and Friend leukemia complex infections on background antibody-forming cells to various erythrocytes.", "content": "The numbers of background antibody-forming cells (BPFC) toward erythrocytes of various species present in the lymphoid organs of unimmunized susceptible BALB/c and resistant C57BL/6 mice were investigated at various times after infection with Friend leukemia complex (FLC) or Rowson-Parr virus (RPV). Both virus preparations induced an increase of BPFC numbers in both animal strains, but the rate and magnitude of the enhancements produced by RPV were much lower. The degree of potentiation varied with the specificity of the BPFC populations and was more pronounced in the spleen than in the lymph nodes and in BALB/c than in C57BL/6 mice. In the late stage of FLC infection, the numbers of splenic BPFC to some erythrocytes underwent a dramatic fall, which was not observed in RPV-infected mice. BPFC present in BALB/c splenocytes cultured in diffusion chambers implanted in the peritoneal cavity of isogeneic normal mice were not affected by viral infection of the chambers.", "contents": "Immunodepression by Rowson-Parr virus in mice: effect of Rowson-Parr virus and Friend leukemia complex infections on background antibody-forming cells to various erythrocytes. The numbers of background antibody-forming cells (BPFC) toward erythrocytes of various species present in the lymphoid organs of unimmunized susceptible BALB/c and resistant C57BL/6 mice were investigated at various times after infection with Friend leukemia complex (FLC) or Rowson-Parr virus (RPV). Both virus preparations induced an increase of BPFC numbers in both animal strains, but the rate and magnitude of the enhancements produced by RPV were much lower. The degree of potentiation varied with the specificity of the BPFC populations and was more pronounced in the spleen than in the lymph nodes and in BALB/c than in C57BL/6 mice. In the late stage of FLC infection, the numbers of splenic BPFC to some erythrocytes underwent a dramatic fall, which was not observed in RPV-infected mice. BPFC present in BALB/c splenocytes cultured in diffusion chambers implanted in the peritoneal cavity of isogeneic normal mice were not affected by viral infection of the chambers."}
{"id": "PMID:1091559", "title": "Heteroimmunization to the capsular polysaccharide of Haemophilus influenzae type b induced by enteric cross-reacting bacteria.", "content": "Cross-reacting Escherichia coli strains Easter and 89 and Bacillus pumilis fed to newborn rabbits and E. coli fed to adult rhesus monkeys did not exert untoward reactions. The E. coli regularly colonized the newborns' intestinal tract from 1 to 7 weeks. High doses of E. coli were necessary to colonize adult primates. Colonization occurred in fewer newborn rabbits and lasted only 1 to 3 weeks with B. pumilis. Colonized newborn rabbits and adult rhesus had an active Haemophilus influenzae type b (HITB) immune response. In the rabbit, colonization resulted in accelerated induction of immunoglobulin (Ig) M-. IgA-, and IgG-producing cells in the spleen, mesenteric lymph nodes, and Peyer's patches after HITB challenge. E. coli-fed and control newborn primates were naturally colonized with nasopharyngeal and enteric cross-reacting bacteria and both groups rapidly developed HITB antibodies in the absence of the homologous organisms. Human newborn stool cultures, taken at the time of discharge from the nursery, showed a 0.9% carriage rate for cross-reacting E. coli. These \"carrier\" infants acquired HITB antibodies more rapidly than their age-matched \"noncarrier\" controls.", "contents": "Heteroimmunization to the capsular polysaccharide of Haemophilus influenzae type b induced by enteric cross-reacting bacteria. Cross-reacting Escherichia coli strains Easter and 89 and Bacillus pumilis fed to newborn rabbits and E. coli fed to adult rhesus monkeys did not exert untoward reactions. The E. coli regularly colonized the newborns' intestinal tract from 1 to 7 weeks. High doses of E. coli were necessary to colonize adult primates. Colonization occurred in fewer newborn rabbits and lasted only 1 to 3 weeks with B. pumilis. Colonized newborn rabbits and adult rhesus had an active Haemophilus influenzae type b (HITB) immune response. In the rabbit, colonization resulted in accelerated induction of immunoglobulin (Ig) M-. IgA-, and IgG-producing cells in the spleen, mesenteric lymph nodes, and Peyer's patches after HITB challenge. E. coli-fed and control newborn primates were naturally colonized with nasopharyngeal and enteric cross-reacting bacteria and both groups rapidly developed HITB antibodies in the absence of the homologous organisms. Human newborn stool cultures, taken at the time of discharge from the nursery, showed a 0.9% carriage rate for cross-reacting E. coli. These \"carrier\" infants acquired HITB antibodies more rapidly than their age-matched \"noncarrier\" controls."}
{"id": "PMID:1091560", "title": "Effects of canine distemper virus infection on lymphoid function in vitro and in vivo.", "content": "In the present study, the immunodepressive effects of canine distemper virus (CDV) infection of dogs on two parameters of lymphocyte function, namely phytomitogen-induced cellular proliferation and skin allograft rejection, were investigated. Infection of susceptible gnotobiotic dogs with virulent R252-CDV resulted in a depression of peripheral blood lymphocyte mitogen response as measured by (3H)thymidine incorporation for up to 10 weeks after inoculation. This effect coincided with the appearance of viral antigen by immunofluorescence in leukocytes but persisted after the virus was no longer detectable. Loss of mitogen reactivity was seen in all infected dogs. However, when these same CDV-infected dogs were challenged with foreign skin allografts, no significant retention of grafts over controls was observed despite the depressed lymphocyte activity. Considering the in vitro and in vivo data it was concluded that, although immunodepressive effects of CDV were demonstrated in vitro, paralled in vivo experiments indicated that less than complete suppression of immune functions occurs during the course of CDV infection.", "contents": "Effects of canine distemper virus infection on lymphoid function in vitro and in vivo. In the present study, the immunodepressive effects of canine distemper virus (CDV) infection of dogs on two parameters of lymphocyte function, namely phytomitogen-induced cellular proliferation and skin allograft rejection, were investigated. Infection of susceptible gnotobiotic dogs with virulent R252-CDV resulted in a depression of peripheral blood lymphocyte mitogen response as measured by (3H)thymidine incorporation for up to 10 weeks after inoculation. This effect coincided with the appearance of viral antigen by immunofluorescence in leukocytes but persisted after the virus was no longer detectable. Loss of mitogen reactivity was seen in all infected dogs. However, when these same CDV-infected dogs were challenged with foreign skin allografts, no significant retention of grafts over controls was observed despite the depressed lymphocyte activity. Considering the in vitro and in vivo data it was concluded that, although immunodepressive effects of CDV were demonstrated in vitro, paralled in vivo experiments indicated that less than complete suppression of immune functions occurs during the course of CDV infection."}
{"id": "PMID:1091561", "title": "Superinfection in mice previously infected with Mycobacterium leprae.", "content": "Previous studies of the protection of mice by prior infection with Mycobacterium leprae in one hind footpad against challenge with M.leprae in the opposite hind footpad had produced conflicting results; therefore, the problem was restudied. In several experiments, BALB/c mice were inoculated first in the right hind footpad with 5,000 M. leprae and then challenged in the left hind footpad with 5,000 M. leprae of the same strain at intervals after primary infection, at the same time that uninfected mice were inoculated. Multiplication of the M. leprae of the secondary challenge inoculum occurred at the same rate and to the same level as multiplication in uninfected mice when challenges were made soon after primary infection. Multiplication was slowed but proceeded to the same level in previously infected as in uninfected mice when the challenges were administered between 76 and 106 days after primary infection (47 to 17 days before the M. leprae of the primary inoculum had multiplied to the level of 10-6 organisms per footpad). Finally, the M. leprae of a secondary challenge administered at the time that the organisms of the primary inoculum had multiplied to 10-6 per footpad or later not only multiplied more slowly in previously infected than in control animals, but multiplication in the previously infected animals reached a lower maximum. These results are similar to those observed when mice previously infected with M. bovis (BCG), M. marinum, Toxoplasma gondii, or Besnoitia jellisoni were challenged with M. leprae.", "contents": "Superinfection in mice previously infected with Mycobacterium leprae. Previous studies of the protection of mice by prior infection with Mycobacterium leprae in one hind footpad against challenge with M.leprae in the opposite hind footpad had produced conflicting results; therefore, the problem was restudied. In several experiments, BALB/c mice were inoculated first in the right hind footpad with 5,000 M. leprae and then challenged in the left hind footpad with 5,000 M. leprae of the same strain at intervals after primary infection, at the same time that uninfected mice were inoculated. Multiplication of the M. leprae of the secondary challenge inoculum occurred at the same rate and to the same level as multiplication in uninfected mice when challenges were made soon after primary infection. Multiplication was slowed but proceeded to the same level in previously infected as in uninfected mice when the challenges were administered between 76 and 106 days after primary infection (47 to 17 days before the M. leprae of the primary inoculum had multiplied to the level of 10-6 organisms per footpad). Finally, the M. leprae of a secondary challenge administered at the time that the organisms of the primary inoculum had multiplied to 10-6 per footpad or later not only multiplied more slowly in previously infected than in control animals, but multiplication in the previously infected animals reached a lower maximum. These results are similar to those observed when mice previously infected with M. bovis (BCG), M. marinum, Toxoplasma gondii, or Besnoitia jellisoni were challenged with M. leprae."}
{"id": "PMID:1091562", "title": "Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.", "content": "The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. In addition, T. pallidum attained intracellularity as early as 30 min after inoculation of the cell monolayers. In sharp contrast, T. phagedenis biotype Reiter and T. denticola did not attach and did not enter cultured cells. Most importantly, intracellular and/or attached T. pallidum retained virulence for at least 24 h. Similar observations of attachment and retention of virulence were detected with ME-180, a cell line derived from a human cervical carcinoma. Preliminary studies with superoxide dismutase indicated that this enzyme prolonged treponemal motility and retention of virulence in the presence of cultured cells. These data provide guidelines for further investigations of in vitro cultivation of T. pallidum.", "contents": "Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. In addition, T. pallidum attained intracellularity as early as 30 min after inoculation of the cell monolayers. In sharp contrast, T. phagedenis biotype Reiter and T. denticola did not attach and did not enter cultured cells. Most importantly, intracellular and/or attached T. pallidum retained virulence for at least 24 h. Similar observations of attachment and retention of virulence were detected with ME-180, a cell line derived from a human cervical carcinoma. Preliminary studies with superoxide dismutase indicated that this enzyme prolonged treponemal motility and retention of virulence in the presence of cultured cells. These data provide guidelines for further investigations of in vitro cultivation of T. pallidum."}
{"id": "PMID:1091563", "title": "Motility as a virulence factor for Vibrio cholerae.", "content": "The ability of motile strains of the Ogawa and Inaba serotypes of classical Vibrio cholerae and of the El Tor biotypes to kill suckling mice after oral challenge with 10-8 colony-forming units (representing at least 100 to 1,000 minimal lethal doses) was compared to that of nonmotile derivatives of the same strains. Loss of motility, in each case, resulted in a marked reduction in virulence. The mortality (at 36 h) caused by 10 of the 13 nonmotile strains was 32% or less. whereas the motile wild-type strains resulted in nearly 100% deaths. The reduced virulence of the nonmotile strains was associated with reduced capacity to adsorb to the surface of segments of mouse intestine. The mutants were tested for alterations in enterotoxin production and surface properties. The results suggest that motility may contribute to virulence by increasing the chance for association of the vibrios with the intestinal mucosa.", "contents": "Motility as a virulence factor for Vibrio cholerae. The ability of motile strains of the Ogawa and Inaba serotypes of classical Vibrio cholerae and of the El Tor biotypes to kill suckling mice after oral challenge with 10-8 colony-forming units (representing at least 100 to 1,000 minimal lethal doses) was compared to that of nonmotile derivatives of the same strains. Loss of motility, in each case, resulted in a marked reduction in virulence. The mortality (at 36 h) caused by 10 of the 13 nonmotile strains was 32% or less. whereas the motile wild-type strains resulted in nearly 100% deaths. The reduced virulence of the nonmotile strains was associated with reduced capacity to adsorb to the surface of segments of mouse intestine. The mutants were tested for alterations in enterotoxin production and surface properties. The results suggest that motility may contribute to virulence by increasing the chance for association of the vibrios with the intestinal mucosa."}
{"id": "PMID:1091564", "title": "Serotyping of Chlamydia: isolates of bovine origin.", "content": "Chlamydial isolates of bovine origin were serotyped by a plaque reduction method. Of the two major serotypes observed, type 1 included isolates from bovine abortion and enteric infections, whereas type 2 isolates were associated with polyarthritis or encephalomyelitis. These two serotypes were identical to those with a similar disease distribution previously observed in isolates of ovine origin. The two groups did not cross-react and they were serologically unrelated to chlamydiae of avian origin. Thus, it appears that many chlamydial isolates causing intestinal infections or abortion in sheep or cattle are closely related antigenically, as are those producing polyarthritis, encephalomyelitis, and conjunctivitis, and that the two groups are distinct.", "contents": "Serotyping of Chlamydia: isolates of bovine origin. Chlamydial isolates of bovine origin were serotyped by a plaque reduction method. Of the two major serotypes observed, type 1 included isolates from bovine abortion and enteric infections, whereas type 2 isolates were associated with polyarthritis or encephalomyelitis. These two serotypes were identical to those with a similar disease distribution previously observed in isolates of ovine origin. The two groups did not cross-react and they were serologically unrelated to chlamydiae of avian origin. Thus, it appears that many chlamydial isolates causing intestinal infections or abortion in sheep or cattle are closely related antigenically, as are those producing polyarthritis, encephalomyelitis, and conjunctivitis, and that the two groups are distinct."}
{"id": "PMID:1091565", "title": "Inhibition of the steroidogenic effects of cholera and heat-labile Escherichia coli enterotoxins by GM1 ganglioside: evidence for a similar receptor site for the two toxins.", "content": "The effects of three different ganglioside preparations on cholera enterotoxin (CT) and heat-labile Escherichia coli enterotoxin (ECT)-induced steroidogenesis in Y1 and OS3 adrenal tumor cells in tissue culture were examined. Only with GM1 ganglioside was any inhibition of the toxins' effects noted. Concentrations of the crude ECT preparation that gave similar morphogenic and steroidogenic effects as CT were inhibited by the same amount or less of GM1 as that required to inhibit the effects of CT. The results of competition experiments also demonstrated that previous incubation of GM1 with one toxin could inhibit the ganglioside's ability to inactivate the other toxin. These findings indicate that at least for Y1 and OS3 adrenal tumor cells, GM1 may resemble or be the receptor for both CT and ECT.", "contents": "Inhibition of the steroidogenic effects of cholera and heat-labile Escherichia coli enterotoxins by GM1 ganglioside: evidence for a similar receptor site for the two toxins. The effects of three different ganglioside preparations on cholera enterotoxin (CT) and heat-labile Escherichia coli enterotoxin (ECT)-induced steroidogenesis in Y1 and OS3 adrenal tumor cells in tissue culture were examined. Only with GM1 ganglioside was any inhibition of the toxins' effects noted. Concentrations of the crude ECT preparation that gave similar morphogenic and steroidogenic effects as CT were inhibited by the same amount or less of GM1 as that required to inhibit the effects of CT. The results of competition experiments also demonstrated that previous incubation of GM1 with one toxin could inhibit the ganglioside's ability to inactivate the other toxin. These findings indicate that at least for Y1 and OS3 adrenal tumor cells, GM1 may resemble or be the receptor for both CT and ECT."}
{"id": "PMID:1091566", "title": "Detection of virus-specific antibody-forming cells of mice immunized with Newcastle disease virus.", "content": "The hemolytic plaque assay was adapted to the detection of antibodies to Newcastle disease virus (NDV) in an in vivo, an in vitro system, and a combined in vivo-in vitro system. Several conditions were tested for coupling of sheep erythrocytes to NDV and for the kinetics of plaque formation in the in vivo and in vitro systems. The one set of conditions which provided the best responses is presented. The effect of multiple injections of NDV into mice on plaque formation was optimized.", "contents": "Detection of virus-specific antibody-forming cells of mice immunized with Newcastle disease virus. The hemolytic plaque assay was adapted to the detection of antibodies to Newcastle disease virus (NDV) in an in vivo, an in vitro system, and a combined in vivo-in vitro system. Several conditions were tested for coupling of sheep erythrocytes to NDV and for the kinetics of plaque formation in the in vivo and in vitro systems. The one set of conditions which provided the best responses is presented. The effect of multiple injections of NDV into mice on plaque formation was optimized."}
{"id": "PMID:1091567", "title": "On the question of permeability of the blood-brain barrier to botulinum toxin.", "content": "The clinical symptoms of botulinum intoxication suggest that besides the involvement of the peripheral nervous system, the central nervous system is also affected. Studies were undertaken to determine whether pure toxin of Clostridium botulinum type A could be demonstrated in the brains of poisoned mice. With the aid of autoradiography of the toxin marked with 125-I and indirect fluorescent labeling it was possible to show the presence of the toxin in the parenchyma of the brain.", "contents": "On the question of permeability of the blood-brain barrier to botulinum toxin. The clinical symptoms of botulinum intoxication suggest that besides the involvement of the peripheral nervous system, the central nervous system is also affected. Studies were undertaken to determine whether pure toxin of Clostridium botulinum type A could be demonstrated in the brains of poisoned mice. With the aid of autoradiography of the toxin marked with 125-I and indirect fluorescent labeling it was possible to show the presence of the toxin in the parenchyma of the brain."}
{"id": "PMID:1091570", "title": "Infection versus disease in South American blastomycosis.", "content": "The mechanism of infection in South American blastomycosis is discussed, and the probability of a mild or asymptomatic form of the disease, regressing spontaneously with subsequent immunity, in persons living in endemic areas. Primary lesions in the oral mucosa or in the skin have not been convincingly shown to occur in paracoccidioidomycosis. Several reports of systemic involvement of many organs with no evidence of skin and mucosal lesions support the theory of pulmonary entry.", "contents": "Infection versus disease in South American blastomycosis. The mechanism of infection in South American blastomycosis is discussed, and the probability of a mild or asymptomatic form of the disease, regressing spontaneously with subsequent immunity, in persons living in endemic areas. Primary lesions in the oral mucosa or in the skin have not been convincingly shown to occur in paracoccidioidomycosis. Several reports of systemic involvement of many organs with no evidence of skin and mucosal lesions support the theory of pulmonary entry."}
{"id": "PMID:1091573", "title": "The effects of rifampicin on electron- and neutron-irradiated E. coli B/r and BS-1: Survival, DNA degradation and DNA synthesis by membrane fragments.", "content": "The effect of rifampicin after irradiation with electrons or neutrons on cell-killing, DNA degradation and the ability of DNA-membrane complex extracted from irradiated bacteria to synthesize DNA was studied in strains E. coli B/r and BS minus 1. Post-irradiation treatment with rifampicin greatly enhanced the lethal effects of both electrons and neutrons on the repair-proficient strain E. coli B/r under anoxic conditions. However, the same post-irradiation treatment of the repair-deficient strain E. coli BS minus 1 with rifampicin gave no consequent increase in lethality. Rifampicin did not alter the extent of DNA degradation in either strain after irradiation with electrons or neutrons. The DNA-synthetic activity of DNA-membrane complex from electron-irradiated E. coli B/r and BS minus 1 was not altered by post-irradiation treatment with rifampicin, nor did this treatment affect synthesis by DNA-membrane complex isolated from neutron-irradiated BS minus 1; but the complex, isolated from E. coli B/r which had been irradiated under anoxic conditions with neutrons, showed a marked decrease in activity. We suggest that post-irradiation sensitization with rifampicin of the repair-proficient E. coli B/r, especially after irradiation under anoxic conditions, indicates that initiation of the synthesis of an RNA molecule is involved. In addition our results suggest that the structure of the membrane complex in E. coli B/r and BS minus 1 is different.", "contents": "The effects of rifampicin on electron- and neutron-irradiated E. coli B/r and BS-1: Survival, DNA degradation and DNA synthesis by membrane fragments. The effect of rifampicin after irradiation with electrons or neutrons on cell-killing, DNA degradation and the ability of DNA-membrane complex extracted from irradiated bacteria to synthesize DNA was studied in strains E. coli B/r and BS minus 1. Post-irradiation treatment with rifampicin greatly enhanced the lethal effects of both electrons and neutrons on the repair-proficient strain E. coli B/r under anoxic conditions. However, the same post-irradiation treatment of the repair-deficient strain E. coli BS minus 1 with rifampicin gave no consequent increase in lethality. Rifampicin did not alter the extent of DNA degradation in either strain after irradiation with electrons or neutrons. The DNA-synthetic activity of DNA-membrane complex from electron-irradiated E. coli B/r and BS minus 1 was not altered by post-irradiation treatment with rifampicin, nor did this treatment affect synthesis by DNA-membrane complex isolated from neutron-irradiated BS minus 1; but the complex, isolated from E. coli B/r which had been irradiated under anoxic conditions with neutrons, showed a marked decrease in activity. We suggest that post-irradiation sensitization with rifampicin of the repair-proficient E. coli B/r, especially after irradiation under anoxic conditions, indicates that initiation of the synthesis of an RNA molecule is involved. In addition our results suggest that the structure of the membrane complex in E. coli B/r and BS minus 1 is different."}
{"id": "PMID:1091576", "title": "Improved surgical treatment of cancer of the lower lip.", "content": "Combination (with some minor modifications) of the classic plastic procedures of broad resection of the lower lip with V-Y partial closure, specially designed Abbe flap and cheek transposition after a skin resection lateral of the nostril represents a most satisfactory surgical treatment for cancer of the lower lip.", "contents": "Improved surgical treatment of cancer of the lower lip. Combination (with some minor modifications) of the classic plastic procedures of broad resection of the lower lip with V-Y partial closure, specially designed Abbe flap and cheek transposition after a skin resection lateral of the nostril represents a most satisfactory surgical treatment for cancer of the lower lip."}
{"id": "PMID:1091577", "title": "Acute abdomen in a patient treated with lamprene.", "content": "Patients receiving Lamprene may develop acute abdominal symptoms which simulate an abdominal emergency. Withdrawal of the drug relieves these symptoms. The absorption of Lamprene can be increased and deposition in the reticuloendothelial system as crystals can be avoided if it is administered in an alcoholic medium.", "contents": "Acute abdomen in a patient treated with lamprene. Patients receiving Lamprene may develop acute abdominal symptoms which simulate an abdominal emergency. Withdrawal of the drug relieves these symptoms. The absorption of Lamprene can be increased and deposition in the reticuloendothelial system as crystals can be avoided if it is administered in an alcoholic medium."}
{"id": "PMID:1091578", "title": "Radical excision and reconstruction in advanced maxillofacial carcinomas.", "content": "Some problems on excision and reconstruction in maxillofacial cancer surgery were briefly discussed and three representative cases presented. One innovation presented concerned the use of an almost circular cheek skin flap containing branches of the superficial temporalis artery preserved intact.", "contents": "Radical excision and reconstruction in advanced maxillofacial carcinomas. Some problems on excision and reconstruction in maxillofacial cancer surgery were briefly discussed and three representative cases presented. One innovation presented concerned the use of an almost circular cheek skin flap containing branches of the superficial temporalis artery preserved intact."}
{"id": "PMID:1091591", "title": "Excision of the donor cornea instead of enucleation.", "content": "Shortage of corneal grafts is common in many countries.  The method presented here has been developed to relieve this situation and make it easier, especially from the psychological point of view, to obtain a license for excising corneas from cadavers. An 11 mm. corneal button is excised from the donor eye in situ with a Draeger electric rotor trephine and the donor eyes remain apparently normal looking.  These excised corneas without sclerocorneal rims are placed in adequate without sclerocorneal rims are placed in adequate storage media for long-term cryopreservation according to Capella, Kaufman, and Robbins or for short-term storage according to McCarey and Kaufman. The preliminary results are promising and the technique seems not to be too delicate or complicated to be carried out by an experienced eye bank technician.  It has improved our possibilities for storing donor corneas and for collecting many tissue-typed corneas in our eye bank.", "contents": "Excision of the donor cornea instead of enucleation. Shortage of corneal grafts is common in many countries.  The method presented here has been developed to relieve this situation and make it easier, especially from the psychological point of view, to obtain a license for excising corneas from cadavers. An 11 mm. corneal button is excised from the donor eye in situ with a Draeger electric rotor trephine and the donor eyes remain apparently normal looking.  These excised corneas without sclerocorneal rims are placed in adequate without sclerocorneal rims are placed in adequate storage media for long-term cryopreservation according to Capella, Kaufman, and Robbins or for short-term storage according to McCarey and Kaufman. The preliminary results are promising and the technique seems not to be too delicate or complicated to be carried out by an experienced eye bank technician.  It has improved our possibilities for storing donor corneas and for collecting many tissue-typed corneas in our eye bank."}
{"id": "PMID:1091592", "title": "The inhibiting effect of indomethacin on the disruption of the blood-aqueous barrier in the rabbit eye.", "content": "The aqueous flare (AF) of an intact rabbit eye was measured by a photoelectric instrument. Local application of prostaglandin E2 (PGE2) and its precursor arachidonic acid (AA) gave an almost identical increase of the AF. The response to AA but not to PGE2 was inhibited by pretreating the eye locally with a solution of indomethacin. The ability of indomethacin to inhibit the aqueous flare response (AFR) to an agent is assumed to indicate that a kind of prostaglandin is the effector of the AF. Indomethacin blocked the AFR to infrared irradiation of the iris and to intravenous administration of endotoxin but not to subcutaneous administration of alpha-melanocyte-stimulating hormone (alpha-MSH).", "contents": "The inhibiting effect of indomethacin on the disruption of the blood-aqueous barrier in the rabbit eye. The aqueous flare (AF) of an intact rabbit eye was measured by a photoelectric instrument. Local application of prostaglandin E2 (PGE2) and its precursor arachidonic acid (AA) gave an almost identical increase of the AF. The response to AA but not to PGE2 was inhibited by pretreating the eye locally with a solution of indomethacin. The ability of indomethacin to inhibit the aqueous flare response (AFR) to an agent is assumed to indicate that a kind of prostaglandin is the effector of the AF. Indomethacin blocked the AFR to infrared irradiation of the iris and to intravenous administration of endotoxin but not to subcutaneous administration of alpha-melanocyte-stimulating hormone (alpha-MSH)."}
{"id": "PMID:1091593", "title": "The effects of BCG on the dog bladder.", "content": "Immunostimulation with agents such as Bacillus Calmette-Gu\u00e9rin (BCG) may represent an adjunctive treatment modality in patients with cancer of the bladder. To investigate the effects of direct inoculation, BCG was injected into the bladder of sensitized (PPD) and nonsensitized dogs. A marked and predictable inflammatory reaction was seen in all of the sensitized and in some of the nonsensitized dogs. This reaction was characterized by an extensive histiocytic infiltration with varying proportions of polymorphonuclear leukocytes, plasma cells, and lymphocytes. Follow-up examination of one dog 7 weeks after initial biopsy showed that this marked inflammatory reaction had resolved. Our findings suggest that in the future nonspecific immunopotentiators may play a role in the treatment of cancer of the bladder.", "contents": "The effects of BCG on the dog bladder. Immunostimulation with agents such as Bacillus Calmette-Gu\u00e9rin (BCG) may represent an adjunctive treatment modality in patients with cancer of the bladder. To investigate the effects of direct inoculation, BCG was injected into the bladder of sensitized (PPD) and nonsensitized dogs. A marked and predictable inflammatory reaction was seen in all of the sensitized and in some of the nonsensitized dogs. This reaction was characterized by an extensive histiocytic infiltration with varying proportions of polymorphonuclear leukocytes, plasma cells, and lymphocytes. Follow-up examination of one dog 7 weeks after initial biopsy showed that this marked inflammatory reaction had resolved. Our findings suggest that in the future nonspecific immunopotentiators may play a role in the treatment of cancer of the bladder."}
{"id": "PMID:1091594", "title": "Evaluation of a magnetic bladder pump for neurogenic bladders.", "content": "A new bladder pump prosthesis has been designed and evaluated in the normal dog bladder. This encompasses a magnetic pump located subcutaneously with internal silicone catheters from bladder to the urethra. The pump is powered from a hand-held activator which is magnetically coupled externally across the skin. This pump was evaluated in 12 dogs for an average of 65 days and provided adequate emptying of the bladder without alteration of the normal urinary tract or pump malfunction. The potential and limitations of this new approach are discussed.", "contents": "Evaluation of a magnetic bladder pump for neurogenic bladders. A new bladder pump prosthesis has been designed and evaluated in the normal dog bladder. This encompasses a magnetic pump located subcutaneously with internal silicone catheters from bladder to the urethra. The pump is powered from a hand-held activator which is magnetically coupled externally across the skin. This pump was evaluated in 12 dogs for an average of 65 days and provided adequate emptying of the bladder without alteration of the normal urinary tract or pump malfunction. The potential and limitations of this new approach are discussed."}
{"id": "PMID:1091595", "title": "Intrarenal distribution of flow during perfusion.", "content": "Intrarenal flow distribution in ischemic and nonischemic dog kidneys has been studied using the 133Xe washout technique and angiography. These preliminary studies showed two different hemodynamic alterations during perfusion, vasoconstriction, and diminution of cortical flow. Vasoconstriction of the renal artery and branches noted in both groups was observed to be a reversible phenomenon and disappeared within 1 hr of perfusion. A decrease in cortical flow was also seen at the beginning of perfusion in both groups, being much more severe in the ischemic group. Significant improvement in cortical flow was noted during the 1st hr of perfusion by both angiographic and washout techniques. This was most pronounced in the nonischemic group. The determination of flow distribution by 133Xe washout technique appears to correlate well with concomitant angiography. Future studies directed toward modification of preservation methods to minimize these changes may well lead to improvement of the present state of renal preservation.", "contents": "Intrarenal distribution of flow during perfusion. Intrarenal flow distribution in ischemic and nonischemic dog kidneys has been studied using the 133Xe washout technique and angiography. These preliminary studies showed two different hemodynamic alterations during perfusion, vasoconstriction, and diminution of cortical flow. Vasoconstriction of the renal artery and branches noted in both groups was observed to be a reversible phenomenon and disappeared within 1 hr of perfusion. A decrease in cortical flow was also seen at the beginning of perfusion in both groups, being much more severe in the ischemic group. Significant improvement in cortical flow was noted during the 1st hr of perfusion by both angiographic and washout techniques. This was most pronounced in the nonischemic group. The determination of flow distribution by 133Xe washout technique appears to correlate well with concomitant angiography. Future studies directed toward modification of preservation methods to minimize these changes may well lead to improvement of the present state of renal preservation."}
{"id": "PMID:1091604", "title": "Extrachromosomal elements in a super-suppression system of yeast. III. Enhanced detection of weak suppressors in certain non-suppressed psi-strains.", "content": "Individual colonies of an adenine-requiring strain of the genotype ade 2-1, SUPQ5, [psi minus] exhibit high and uniform frequencies of ADE+ revertants (5-1 times 10-5). The frequencies for strains of the genotypes ade 2-1, SUPQ5+, [psi+] and ade 2-1 SUPQ5+, [psi minus] are by contrast 1-2 times 10-7 and 0-5 times 10-7 respectively. Mutation from [psi minus] to [psi+] can occur. This suggests that the original mutation from [psi+] to [psi minus] is not necessarily a physical loss of, or extensive deletion, the extrachromosomal elements. Although mutation from [psi minus] to [psi+] occurs, the majority of ADE+ revertants from ade 2-1, SUPQ5, [psi minus] strains contain other types of mutation. Crosses between most ADE+ revertants and ade 2-1, SUPO5+, [psi minus] strains yield adenine-requiring diploids and tetrad segregations of 0:4, 1:3 and 2:2 ADE+ :ade minus. It is argued that most mutations give rise to weak recessive suppressors (SUPX) which are themselves incapable of suppressing the ochre allele ade 2-1, unless the allele SUPQ5 is also present. Tests with two suppressors, S-theta and S-zeta, isolated by Dr R. A. Gilmore, which are themselves incapable of suppressing ade 2-1, show that they do not do so even in the presence of SUPQ5. Thus the set of suppressors isolated by us is a special class which can perhaps only be observed in the genetic background ade 2-1, SUPQ5, [psi minus]. There is evidence that some of the new suppressors are capable, in the absence of SUPQ5, of weakly suppressing trp 5-48 and perhaps can 1-100. All ochre alleles are, however, suppressed by the combination SUPQ5, SUPX. SUPQ5 is able to suppress trp 5-48, his 5-2, lys 1-1 and can 1-100 in a [psi minus] background, although it does so variably. It is incapable of suppressing ade 2-1 in such a background. It is argued that the alteration in the [psi] determinant results in a lowered efficiency of suppression by SUPQ5 rather than a changed specificity.", "contents": "Extrachromosomal elements in a super-suppression system of yeast. III. Enhanced detection of weak suppressors in certain non-suppressed psi-strains. Individual colonies of an adenine-requiring strain of the genotype ade 2-1, SUPQ5, [psi minus] exhibit high and uniform frequencies of ADE+ revertants (5-1 times 10-5). The frequencies for strains of the genotypes ade 2-1, SUPQ5+, [psi+] and ade 2-1 SUPQ5+, [psi minus] are by contrast 1-2 times 10-7 and 0-5 times 10-7 respectively. Mutation from [psi minus] to [psi+] can occur. This suggests that the original mutation from [psi+] to [psi minus] is not necessarily a physical loss of, or extensive deletion, the extrachromosomal elements. Although mutation from [psi minus] to [psi+] occurs, the majority of ADE+ revertants from ade 2-1, SUPQ5, [psi minus] strains contain other types of mutation. Crosses between most ADE+ revertants and ade 2-1, SUPO5+, [psi minus] strains yield adenine-requiring diploids and tetrad segregations of 0:4, 1:3 and 2:2 ADE+ :ade minus. It is argued that most mutations give rise to weak recessive suppressors (SUPX) which are themselves incapable of suppressing the ochre allele ade 2-1, unless the allele SUPQ5 is also present. Tests with two suppressors, S-theta and S-zeta, isolated by Dr R. A. Gilmore, which are themselves incapable of suppressing ade 2-1, show that they do not do so even in the presence of SUPQ5. Thus the set of suppressors isolated by us is a special class which can perhaps only be observed in the genetic background ade 2-1, SUPQ5, [psi minus]. There is evidence that some of the new suppressors are capable, in the absence of SUPQ5, of weakly suppressing trp 5-48 and perhaps can 1-100. All ochre alleles are, however, suppressed by the combination SUPQ5, SUPX. SUPQ5 is able to suppress trp 5-48, his 5-2, lys 1-1 and can 1-100 in a [psi minus] background, although it does so variably. It is incapable of suppressing ade 2-1 in such a background. It is argued that the alteration in the [psi] determinant results in a lowered efficiency of suppression by SUPQ5 rather than a changed specificity."}
{"id": "PMID:1091610", "title": "A disease in dogs resembling human pemphigus vulgaris: case reports.", "content": "A pemphigus-like disease occurred in 5 dogs. The canine disease closely resembled pemphigus vulgaris in man and was characterized by ulcerative lesions in the oral cavity, on the skin, and at mucocutaneous junctions. Histologically, the first sign of the disease was disappearance of the intercellular bridges in the lower epidermis. The resulting acantholysis was diagnostic for pemphigus. Antibodies to the intercellular space substance were detected by immunofluorescent staining in serum of 4 of the dogs. High doses of corticosteroids were effective in controlling the disease.", "contents": "A disease in dogs resembling human pemphigus vulgaris: case reports. A pemphigus-like disease occurred in 5 dogs. The canine disease closely resembled pemphigus vulgaris in man and was characterized by ulcerative lesions in the oral cavity, on the skin, and at mucocutaneous junctions. Histologically, the first sign of the disease was disappearance of the intercellular bridges in the lower epidermis. The resulting acantholysis was diagnostic for pemphigus. Antibodies to the intercellular space substance were detected by immunofluorescent staining in serum of 4 of the dogs. High doses of corticosteroids were effective in controlling the disease."}
{"id": "PMID:1091611", "title": "Toxicity of hexachlorobenzene and associated residues in edible animal tissues.", "content": "The USDA has detected hexachlorobenzene (HCB) in the body fat of domestic animals and poultry in at least 14 states. In Louisiana, industrial contamination appeared to be largely responsible for the HCB residues detected in cattle. Following the episode in Louisiana, the Environmental Protection Agency (EPA) established an interim tolerance of 0.5 ppm for HCB in the fat of cattle, sheep, goats, and horses. Edible meat products containing HCB at higher concentrations are violative and cannot be used as a source of food for human consumption.", "contents": "Toxicity of hexachlorobenzene and associated residues in edible animal tissues. The USDA has detected hexachlorobenzene (HCB) in the body fat of domestic animals and poultry in at least 14 states. In Louisiana, industrial contamination appeared to be largely responsible for the HCB residues detected in cattle. Following the episode in Louisiana, the Environmental Protection Agency (EPA) established an interim tolerance of 0.5 ppm for HCB in the fat of cattle, sheep, goats, and horses. Edible meat products containing HCB at higher concentrations are violative and cannot be used as a source of food for human consumption."}
{"id": "PMID:1091615", "title": "Methodological aspects of blood flow measurement in ovaries containing corpora lutea.", "content": "Ovarian blood flow measurements were carried out in pseudopregnant anesthetized rabbits in order to compare three different techniques. Direct measurement by venous cannulation and flow determination by 15-mum radioactive microspheres resulted in values 5 and 10 times higher, respectively, than indicator fraction technique using -86Rb. The direct method inevitably involves surgical handling of the ovary and its vessels, which may account for the lower values found with this method than with microspheres. A comparison between the dynamics of -86Rb uptake in the luteal ovary and the hindleg tissues, mainly consisting of striated muscle, indicated that this technique will underestimate ovarian blood flow due to a low ovarian extraction of the radionuclide. In the preparation for direct measurement the relation between blood pressure and flow was linear. This lack of autorgeulation is an important factor to consider in studies of ovarian steroid secretion rates. The radioactive microsphere technique appears to reflect physiological blood flow in the ovary and corpus leteum of the rabbit and can be useful in studies of vascular mechanisms in this gland.", "contents": "Methodological aspects of blood flow measurement in ovaries containing corpora lutea. Ovarian blood flow measurements were carried out in pseudopregnant anesthetized rabbits in order to compare three different techniques. Direct measurement by venous cannulation and flow determination by 15-mum radioactive microspheres resulted in values 5 and 10 times higher, respectively, than indicator fraction technique using -86Rb. The direct method inevitably involves surgical handling of the ovary and its vessels, which may account for the lower values found with this method than with microspheres. A comparison between the dynamics of -86Rb uptake in the luteal ovary and the hindleg tissues, mainly consisting of striated muscle, indicated that this technique will underestimate ovarian blood flow due to a low ovarian extraction of the radionuclide. In the preparation for direct measurement the relation between blood pressure and flow was linear. This lack of autorgeulation is an important factor to consider in studies of ovarian steroid secretion rates. The radioactive microsphere technique appears to reflect physiological blood flow in the ovary and corpus leteum of the rabbit and can be useful in studies of vascular mechanisms in this gland."}
{"id": "PMID:1091616", "title": "Effect of aeration on minimal medium recovery of heated Salmonella typhimurium.", "content": "The effect of presence or absence of air on minimal medium recovery of heated Salmonella typhimurium was investigated. It was determined that the expression of minimal medium recovery is not only dependent on heat and a nutritionally complex medium but also on air. Unlike in the presence of air, in the presence of nitrogen, cells were able to recover their ability to grow on Trypticase soy agar enriched with 0.5% yeast extract (TSY) when incubated in TSY broth. It was established that in the presence of nitrogen the number of heat-TSY- induced, single-straneded breaks in deoxyribonucleic acid (DNA) were less than in the presence of air. Furthermore, the DNA breaks in nitrogen were repaired, whereas DNA breaks in air were not. The ability of cells to grow on TSY agar corresponded well with their ability to repair damage to DNA.", "contents": "Effect of aeration on minimal medium recovery of heated Salmonella typhimurium. The effect of presence or absence of air on minimal medium recovery of heated Salmonella typhimurium was investigated. It was determined that the expression of minimal medium recovery is not only dependent on heat and a nutritionally complex medium but also on air. Unlike in the presence of air, in the presence of nitrogen, cells were able to recover their ability to grow on Trypticase soy agar enriched with 0.5% yeast extract (TSY) when incubated in TSY broth. It was established that in the presence of nitrogen the number of heat-TSY- induced, single-straneded breaks in deoxyribonucleic acid (DNA) were less than in the presence of air. Furthermore, the DNA breaks in nitrogen were repaired, whereas DNA breaks in air were not. The ability of cells to grow on TSY agar corresponded well with their ability to repair damage to DNA."}
{"id": "PMID:1091617", "title": "Regulation of methionine transport activity in Escherichia coli.", "content": "Methionine transport activity in cells of Escherichia coli K-12 is regulated by the  level of the internal methionine pool. Transport activity is depressed either in cells grown in the presence of methionine or in cells exposed to methionine immediately prior to harvest. Alpha-Keto-gamma-methiol-butyrate, D-methionine, or methionine sulfoxide have little effect on the initial rate of uptake of L-methionine when they are added simultaneously with the substrate. However, methionine transport is markedly reduced in cells exposed to these sources of L-methionine before the addition of substrate. This reduction is prevented if the cells are treated with amino oxyacetic acid. The initial rate of uptake into L-methionine-loaded cells was lower than that into unloaded cells. This inhibition affected both methionine transport systems and the inhibition by the internal pool appeared to be non-competitive with the external methionine concentration. Two classes of mutants with increased methionine pools have decreased rates of uptake. Conversely, starvation for methionine in a methionine auxotroph with high rates of methionine degradation resulted in a substantial increase in the rate of methionine transport. Thus, these transport systems are subject to regulation by the internal pool size and possibly by repression.", "contents": "Regulation of methionine transport activity in Escherichia coli. Methionine transport activity in cells of Escherichia coli K-12 is regulated by the  level of the internal methionine pool. Transport activity is depressed either in cells grown in the presence of methionine or in cells exposed to methionine immediately prior to harvest. Alpha-Keto-gamma-methiol-butyrate, D-methionine, or methionine sulfoxide have little effect on the initial rate of uptake of L-methionine when they are added simultaneously with the substrate. However, methionine transport is markedly reduced in cells exposed to these sources of L-methionine before the addition of substrate. This reduction is prevented if the cells are treated with amino oxyacetic acid. The initial rate of uptake into L-methionine-loaded cells was lower than that into unloaded cells. This inhibition affected both methionine transport systems and the inhibition by the internal pool appeared to be non-competitive with the external methionine concentration. Two classes of mutants with increased methionine pools have decreased rates of uptake. Conversely, starvation for methionine in a methionine auxotroph with high rates of methionine degradation resulted in a substantial increase in the rate of methionine transport. Thus, these transport systems are subject to regulation by the internal pool size and possibly by repression."}
{"id": "PMID:1091618", "title": "Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli.", "content": "Specialized lambda transducing phages for the sul+ (supD-) amber suppressor in Escherichia coli K-12 have been isolated, using a secondary site lambda-cI857 lysogen in which we have shown the prophage to be closely linked to sul+.sul+ transducing particles were detected frequently, at 10-5 per plaque-forming unit, in lysates prepared from the secondary-site lysogens. High-frequency transducing lysates were obtained from several independently isolated sul+ transductants and were analyzed by CsCl equilibrium density gradient centrifugation. The transducing phages are defective; marker rescue analysis indicates that the lambda-N gene is not present. In lambda-cI857DELTANdSul+, a bio-type transducing phage, the genes specifying recombination and excision functions have been replaced by bacterial deoxyribonucleic acid.", "contents": "Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli. Specialized lambda transducing phages for the sul+ (supD-) amber suppressor in Escherichia coli K-12 have been isolated, using a secondary site lambda-cI857 lysogen in which we have shown the prophage to be closely linked to sul+.sul+ transducing particles were detected frequently, at 10-5 per plaque-forming unit, in lysates prepared from the secondary-site lysogens. High-frequency transducing lysates were obtained from several independently isolated sul+ transductants and were analyzed by CsCl equilibrium density gradient centrifugation. The transducing phages are defective; marker rescue analysis indicates that the lambda-N gene is not present. In lambda-cI857DELTANdSul+, a bio-type transducing phage, the genes specifying recombination and excision functions have been replaced by bacterial deoxyribonucleic acid."}
{"id": "PMID:1091619", "title": "Deoxyribonucleic acid-cytosine methylation by host- and plasmid-controlled enzymes.", "content": "Deoxyribonucleic acid (DNA)-cytosine methylation specified by the wild-type Escherichia coli K 12 mec+ gene and by the N-3 drug resistance (R) factor was studied in vivo and in vitro. Phage lambda and fd were propagated in the presence of L-[methyl-3H]methionine in various host bacteria. The in vivo labeled DNA was isolated from purified phage and depurinated by formic acid-diphenylamine treatment. The resulting pyrimidine oligonucleotide tracts were separated according to size and base composition by chromatography on diethylaminoethyl-cellulose in 7 M urea at pH 5.5 and 3.5, respectively. The distribution of labeled 5-methylcytosine in DNA pyrimidine tracts was identical for phage grown in mec+ and mec minus (N-3) cells. For phage lambda the major 5-methylcytosine containing tract was the tripyrimidine, C2T; for both fd-mec minus (N-3) DNA and fd-mec+DNA, C2T was the sole 5-methylcytosine-containing tract. When various lambda DNAs were methylated to saturation in vitro by crude extracts from mec+ and mec minus (N-3) cells, the extent of cytosine methylation was the same. This is in contrast to in vivo methylation where lambda-mec minus (N-3) DNA contains twice as many 5-methylcytosines per genome as lambda-mec+ DNA. Therefore, we suggest that the K12 met+ cytosine methylase and the N-3 plasmid modification methylase are capable of recognizing the same nucleotide sequences, but that the in vivo methylation rate is lower in mec+ cells.", "contents": "Deoxyribonucleic acid-cytosine methylation by host- and plasmid-controlled enzymes. Deoxyribonucleic acid (DNA)-cytosine methylation specified by the wild-type Escherichia coli K 12 mec+ gene and by the N-3 drug resistance (R) factor was studied in vivo and in vitro. Phage lambda and fd were propagated in the presence of L-[methyl-3H]methionine in various host bacteria. The in vivo labeled DNA was isolated from purified phage and depurinated by formic acid-diphenylamine treatment. The resulting pyrimidine oligonucleotide tracts were separated according to size and base composition by chromatography on diethylaminoethyl-cellulose in 7 M urea at pH 5.5 and 3.5, respectively. The distribution of labeled 5-methylcytosine in DNA pyrimidine tracts was identical for phage grown in mec+ and mec minus (N-3) cells. For phage lambda the major 5-methylcytosine containing tract was the tripyrimidine, C2T; for both fd-mec minus (N-3) DNA and fd-mec+DNA, C2T was the sole 5-methylcytosine-containing tract. When various lambda DNAs were methylated to saturation in vitro by crude extracts from mec+ and mec minus (N-3) cells, the extent of cytosine methylation was the same. This is in contrast to in vivo methylation where lambda-mec minus (N-3) DNA contains twice as many 5-methylcytosines per genome as lambda-mec+ DNA. Therefore, we suggest that the K12 met+ cytosine methylase and the N-3 plasmid modification methylase are capable of recognizing the same nucleotide sequences, but that the in vivo methylation rate is lower in mec+ cells."}
{"id": "PMID:1091620", "title": "Stimulation of adenosine 5'-triphosphate-dependent in vitro deoxyribonucleic acid replication by factors from the periplasmic space of Escherichia coli.", "content": "In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode, of in vitro DNA synthesis is exhibited which is sensitivie to nalidixic acid.  These systems require growth of the agar-embedded cells in a preincubation medium before spheroplast formation and osmotic lysis. Inhibitor studies suggest that one or more required macromolecular species are synthesized during this preincubation growth period. Osmotic shock fluid from E. coli contains macromolecular factors which preferentially stimulate the ATP- dependent semiconservative mode of in vitro DNA synthesis. In some cases, the ATP independent mode of synthesis is inhibited by shock fluid. Evidence is presented that the stimulating factors found in the osmotic shock fluid come from the E. coli periplasmic space. This stimulation is observed using either toluene-treated cells or lysed agar-embedded ethylene glycol-bis-(beta-aminoethyl ether) N,N'-tetraacetate-lysozyme spheroplasts, and is thus independent of the in vitro DNA synthesis system used. Shock fluid obtained from a given E. coli dna mutant does not stimulate in vitro DNA synthesis by that mutant. However, in some cases, shock fluid from one class of dna mutants does stimulate ATP dependent in vitro DNA synthesis by another class of dna mutants, in a thermosensitive reacaction. Gently prepared cell extracts also stimulate ATP-dependent in vitro DNA synthesis, whereas cell extracts prepared by more severe procedures inhibit this in vitro synthesis. Severl stimulating DNA replication factors may be present in the osmotic shock fluid, including products of E. coli dna genes.", "contents": "Stimulation of adenosine 5'-triphosphate-dependent in vitro deoxyribonucleic acid replication by factors from the periplasmic space of Escherichia coli. In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode, of in vitro DNA synthesis is exhibited which is sensitivie to nalidixic acid.  These systems require growth of the agar-embedded cells in a preincubation medium before spheroplast formation and osmotic lysis. Inhibitor studies suggest that one or more required macromolecular species are synthesized during this preincubation growth period. Osmotic shock fluid from E. coli contains macromolecular factors which preferentially stimulate the ATP- dependent semiconservative mode of in vitro DNA synthesis. In some cases, the ATP independent mode of synthesis is inhibited by shock fluid. Evidence is presented that the stimulating factors found in the osmotic shock fluid come from the E. coli periplasmic space. This stimulation is observed using either toluene-treated cells or lysed agar-embedded ethylene glycol-bis-(beta-aminoethyl ether) N,N'-tetraacetate-lysozyme spheroplasts, and is thus independent of the in vitro DNA synthesis system used. Shock fluid obtained from a given E. coli dna mutant does not stimulate in vitro DNA synthesis by that mutant. However, in some cases, shock fluid from one class of dna mutants does stimulate ATP dependent in vitro DNA synthesis by another class of dna mutants, in a thermosensitive reacaction. Gently prepared cell extracts also stimulate ATP-dependent in vitro DNA synthesis, whereas cell extracts prepared by more severe procedures inhibit this in vitro synthesis. Severl stimulating DNA replication factors may be present in the osmotic shock fluid, including products of E. coli dna genes."}
{"id": "PMID:1091621", "title": "Gentic mapping of Salmonella typhimurium peptidase mutations.", "content": "The map positions of three loci, each specifying a different peptidase, have been determined in Salmonella typhimurium. Mutations in pepN (leading to loss of peptidase N [1974] are co-transducible with pyrD. The order of markers in this region is put pyrD pepN. Mutations in pepA (leading to loss of peptidase A [1974] are co-transducible with pyrB and argI. The relative orientation of these markers is pepA argI pyrB. Mutations in pepDP (leading to loss of dipeptidase, peptidase D) are co-transducible with proBA and gxu. The order of these markers is pepD gxu pro.", "contents": "Gentic mapping of Salmonella typhimurium peptidase mutations. The map positions of three loci, each specifying a different peptidase, have been determined in Salmonella typhimurium. Mutations in pepN (leading to loss of peptidase N [1974] are co-transducible with pyrD. The order of markers in this region is put pyrD pepN. Mutations in pepA (leading to loss of peptidase A [1974] are co-transducible with pyrB and argI. The relative orientation of these markers is pepA argI pyrB. Mutations in pepDP (leading to loss of dipeptidase, peptidase D) are co-transducible with proBA and gxu. The order of these markers is pepD gxu pro."}
{"id": "PMID:1091622", "title": "Characterization of mucoid mutants of Escherichia coli K-12 isolated after exposure to ozone.", "content": "Seventy mucoid mutants retaining the phenotypic properties of the ultraviolet-induced lon strains of Escherichia coli K-12 were isolated after treatment of strain MQ259 (lon+) with ozone. They produce mucoid colonies at 37 C on minimal agar, are abnormally sensitive to radiation, and tend to grow in long aseptate filaments after irradiation with ultraviolet light. Results indicate also that ozone sensitivity, radiosensitivity, and mucoidy are pleiotropic properties of the lon gene.", "contents": "Characterization of mucoid mutants of Escherichia coli K-12 isolated after exposure to ozone. Seventy mucoid mutants retaining the phenotypic properties of the ultraviolet-induced lon strains of Escherichia coli K-12 were isolated after treatment of strain MQ259 (lon+) with ozone. They produce mucoid colonies at 37 C on minimal agar, are abnormally sensitive to radiation, and tend to grow in long aseptate filaments after irradiation with ultraviolet light. Results indicate also that ozone sensitivity, radiosensitivity, and mucoidy are pleiotropic properties of the lon gene."}
{"id": "PMID:1091623", "title": "Tryptophan photoproduct(s): sensitized induction of strand breaks (or alkali-labile bonds) in bacterial deoxyribonucleic acid during near-ultraviolet irradiation.", "content": "Long-wavelength ultraviolet light (300 to 400 nm) converts L-tryptophan to a photoproduct that is toxic for bacterial cells in dark conditions. We now report that similar photoproducts of l-tryptophan sensitize bacterial deoxyribonucleic acid to 365-nm radiation, increasing the yield of deoxyribonucleic acid strand breaks (or alkali-labile bonds) by approximately 11.5-fold. Evidence is also presented which indicates that thse sensitized deoxyribonucleic acid lesions contribute to lethality for Escherichia coli irradiated with 365-nm ultraviolet light in suspensions of tryptophan photoproducts.", "contents": "Tryptophan photoproduct(s): sensitized induction of strand breaks (or alkali-labile bonds) in bacterial deoxyribonucleic acid during near-ultraviolet irradiation. Long-wavelength ultraviolet light (300 to 400 nm) converts L-tryptophan to a photoproduct that is toxic for bacterial cells in dark conditions. We now report that similar photoproducts of l-tryptophan sensitize bacterial deoxyribonucleic acid to 365-nm radiation, increasing the yield of deoxyribonucleic acid strand breaks (or alkali-labile bonds) by approximately 11.5-fold. Evidence is also presented which indicates that thse sensitized deoxyribonucleic acid lesions contribute to lethality for Escherichia coli irradiated with 365-nm ultraviolet light in suspensions of tryptophan photoproducts."}
{"id": "PMID:1091624", "title": "Maltose chemoreceptor of Escherichia coli.", "content": "Strains carrying mutations in the maltose system of Escherichia coli were assayed for maltose taxis, maltose uptake at 1 and 10 muM maltose, and maltose-binding activity released by osmotic shock. An earlier conclusion that the metabolism of maltose is not necessary for chemoreception is extended to include the functioning of maltodextrin phosphorylase, the product of malP, and the genetic control of the maltose receptor by the product of malT is confirmed. Mutants in malF and malK are defective in maltose transport at low concentrations as well as high concentrations, as previously shown, but are essentially normal in maltose taxis. The product of malE has been previously shown to be the maltose-binding protein and was implicated in maltose transport. Most malE mutants are defective in maltose taxis, and all those tested are defective in maltose transport at low concentrations. Thus, as previously suggested, the maltose-binding protein probably serves as the recognition component of the maltose receptor, as well as a component of the transport system. tsome malE mutants release maltose-binding activity and are tactic toward maltose, although defective in maltose transport, implying that the binding protein has separate sites for interaction with the chemotaxis and transport systems. Some mutations in lamB, whose product is the receptor for the bacteriophage lamba, cause defects in maltose taxis, indicating some involvement of that product in maltose reception.", "contents": "Maltose chemoreceptor of Escherichia coli. Strains carrying mutations in the maltose system of Escherichia coli were assayed for maltose taxis, maltose uptake at 1 and 10 muM maltose, and maltose-binding activity released by osmotic shock. An earlier conclusion that the metabolism of maltose is not necessary for chemoreception is extended to include the functioning of maltodextrin phosphorylase, the product of malP, and the genetic control of the maltose receptor by the product of malT is confirmed. Mutants in malF and malK are defective in maltose transport at low concentrations as well as high concentrations, as previously shown, but are essentially normal in maltose taxis. The product of malE has been previously shown to be the maltose-binding protein and was implicated in maltose transport. Most malE mutants are defective in maltose taxis, and all those tested are defective in maltose transport at low concentrations. Thus, as previously suggested, the maltose-binding protein probably serves as the recognition component of the maltose receptor, as well as a component of the transport system. tsome malE mutants release maltose-binding activity and are tactic toward maltose, although defective in maltose transport, implying that the binding protein has separate sites for interaction with the chemotaxis and transport systems. Some mutations in lamB, whose product is the receptor for the bacteriophage lamba, cause defects in maltose taxis, indicating some involvement of that product in maltose reception."}
{"id": "PMID:1091625", "title": "Origin of the TEM-beta-lactamase gene found on plasmids.", "content": "A sequence of deoxyribonucleic acid of 2.7 times 10-6 to 3.3 times 10-6 daltons which includes the TEM beta-lactamase gene is present on the small plasmid RSF 1030 (R-Amp). This same sequence is present on plasmid derivatives that have received a translocation of deoxyribonucleic acid specifying the TEM beta-lactamase and is also present on naturally occurring plasmids of the F1, F11, N, X, O, I, C, and W incompatibility groups that do not specify ampicillin resistance or specify O-type beta-lactamases.", "contents": "Origin of the TEM-beta-lactamase gene found on plasmids. A sequence of deoxyribonucleic acid of 2.7 times 10-6 to 3.3 times 10-6 daltons which includes the TEM beta-lactamase gene is present on the small plasmid RSF 1030 (R-Amp). This same sequence is present on plasmid derivatives that have received a translocation of deoxyribonucleic acid specifying the TEM beta-lactamase and is also present on naturally occurring plasmids of the F1, F11, N, X, O, I, C, and W incompatibility groups that do not specify ampicillin resistance or specify O-type beta-lactamases."}
{"id": "PMID:1091626", "title": "Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases.", "content": "Seven transfer ribonucleic acid (tRNA) methylase mutants were isolated from Escherichia coli K-12 by examining the ability of RNA prepared from clones of unselected mutagenized cells to accept methyl groups from S-adenosylmethionine catalyzed by crude enzymes from wild-type cells. Five of the mutants had an altered uracil-tRNA methylase; consequently their tRNA's lacked ribothymidine. One mutant had tRNA deficient in 7-methylguanosine, and one mutant contained tRNA lacking 2-thio-5-methylaminomethyluridine. The genetic loci of the three tRNA methylase mutants were distributed over the E. coli genome. The mutant strain deficient in 7-methylguanosine biosynthesis showed a reduced efficiency in the suppression of amber mutations carried by T4 or lambda phages.", "contents": "Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. Seven transfer ribonucleic acid (tRNA) methylase mutants were isolated from Escherichia coli K-12 by examining the ability of RNA prepared from clones of unselected mutagenized cells to accept methyl groups from S-adenosylmethionine catalyzed by crude enzymes from wild-type cells. Five of the mutants had an altered uracil-tRNA methylase; consequently their tRNA's lacked ribothymidine. One mutant had tRNA deficient in 7-methylguanosine, and one mutant contained tRNA lacking 2-thio-5-methylaminomethyluridine. The genetic loci of the three tRNA methylase mutants were distributed over the E. coli genome. The mutant strain deficient in 7-methylguanosine biosynthesis showed a reduced efficiency in the suppression of amber mutations carried by T4 or lambda phages."}
{"id": "PMID:1091627", "title": "Chromosomal location of mutations affecting the electrophoretic mobility of malate dehydrogenase in Escherichia coli K-12.", "content": "The structural locus for a soluble malate dehydrogenase (l-malate:NAD oxidoreductase, EC 1.1.1.37), mdh, lies about 1.2 min from aspB on the Escherichia coli chromosome in the sequence argG, aspB, mdh.", "contents": "Chromosomal location of mutations affecting the electrophoretic mobility of malate dehydrogenase in Escherichia coli K-12. The structural locus for a soluble malate dehydrogenase (l-malate:NAD oxidoreductase, EC 1.1.1.37), mdh, lies about 1.2 min from aspB on the Escherichia coli chromosome in the sequence argG, aspB, mdh."}
{"id": "PMID:1091628", "title": "Analysis of glutamate exit in Escherichia coli.", "content": "Escherichia coli B exhibits carrier-mediated first-order exit of glutamate with a half-time of less than 4 min, similar to that observed in K-12 strains. Glutamate exit in both B and K-12 strains is inhibited by arsenite. Practically all of the radioactivity lost during exit by K-12 cells has been accounted for as glutamate in the cell filtrate.", "contents": "Analysis of glutamate exit in Escherichia coli. Escherichia coli B exhibits carrier-mediated first-order exit of glutamate with a half-time of less than 4 min, similar to that observed in K-12 strains. Glutamate exit in both B and K-12 strains is inhibited by arsenite. Practically all of the radioactivity lost during exit by K-12 cells has been accounted for as glutamate in the cell filtrate."}
{"id": "PMID:1091629", "title": "Modified penicillin enrichment procedure for the selection of bacterial mutants.", "content": "Penicillin enrichment and selection of biochemical mutants was performed on agar plates. With this technique, there is an optimum penicillin exposure time for the greatest yield of a particular auxotroph.", "contents": "Modified penicillin enrichment procedure for the selection of bacterial mutants. Penicillin enrichment and selection of biochemical mutants was performed on agar plates. With this technique, there is an optimum penicillin exposure time for the greatest yield of a particular auxotroph."}
{"id": "PMID:1091630", "title": "Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells carrying a derepressed F-like R factor.", "content": "The outer membranes of Escherichia coli K-12 cells carrying a derepressed F-like R factor contain about 7 times 10-4 molecules per cell of a protein similar to the subunit of the sex pili specified by the R factor. This protein pool is absent in cells carrying the repressed variant of the R factor. The size of the pool is about one-half of the amount of protein incorporated into mature sex pili at the peak of production and is independent of the phase of growth of the culture. The molecular weight of the protein in the pool and of the subunit of the sex pili specified by the cells is 12,500 plus or minus 600.", "contents": "Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells carrying a derepressed F-like R factor. The outer membranes of Escherichia coli K-12 cells carrying a derepressed F-like R factor contain about 7 times 10-4 molecules per cell of a protein similar to the subunit of the sex pili specified by the R factor. This protein pool is absent in cells carrying the repressed variant of the R factor. The size of the pool is about one-half of the amount of protein incorporated into mature sex pili at the peak of production and is independent of the phase of growth of the culture. The molecular weight of the protein in the pool and of the subunit of the sex pili specified by the cells is 12,500 plus or minus 600."}
{"id": "PMID:1091631", "title": "Properties of alpha-dehydrobiotin-resistant mutants of Escherichia coli K-12.", "content": "We have isolated four classes of mutants resistant to alpha-dehydrobiotin, a biotin analogue. One mutant group, referred to as bioR shows high excretion levels of biotin vitamers, derepressed levels of the biotin biosynthetic enzymes, and resistance to repression by biotin. The mutation has been mapped between argC and bfe at min 79. A second class  of mutants, with lesions in the bioA operon at min 17.5, shows derepressed levels of the dethiobiotin synthetase enzyme and has been tentatively designated as bioO mutants. The other two mutant groups  show alterations in permeability: biotin uptake is markedly reduced in one, whereas in the other proline uptake is also affected. The former mutation lies near metE at min 75 and has been designated as bioP. The permeability mutants in the second group also show poor growth on minimal media, suggesting a generalized permeability effect. This mutation, designated as P, has not been mapped.", "contents": "Properties of alpha-dehydrobiotin-resistant mutants of Escherichia coli K-12. We have isolated four classes of mutants resistant to alpha-dehydrobiotin, a biotin analogue. One mutant group, referred to as bioR shows high excretion levels of biotin vitamers, derepressed levels of the biotin biosynthetic enzymes, and resistance to repression by biotin. The mutation has been mapped between argC and bfe at min 79. A second class  of mutants, with lesions in the bioA operon at min 17.5, shows derepressed levels of the dethiobiotin synthetase enzyme and has been tentatively designated as bioO mutants. The other two mutant groups  show alterations in permeability: biotin uptake is markedly reduced in one, whereas in the other proline uptake is also affected. The former mutation lies near metE at min 75 and has been designated as bioP. The permeability mutants in the second group also show poor growth on minimal media, suggesting a generalized permeability effect. This mutation, designated as P, has not been mapped."}
{"id": "PMID:1091632", "title": "Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli.", "content": "An efficient method for isolation of a large number of plasmid mutants is described. It is based on the fact that N-methyl-N'-nitro-N-nitrosoguanidine induces a number of closely linked mutations within a short segment of the bacterial chromosome. Thus, selection for reversions of an auxotrophic marker located on the ColVBtrp plasmid yielded a large fraction (more than 50 percent) of mutants defective in some plasmid functions, including its own maintenance in the host bacteria. The results of preliminary characterization of strains carrying these mutated plasmids are presented.", "contents": "Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli. An efficient method for isolation of a large number of plasmid mutants is described. It is based on the fact that N-methyl-N'-nitro-N-nitrosoguanidine induces a number of closely linked mutations within a short segment of the bacterial chromosome. Thus, selection for reversions of an auxotrophic marker located on the ColVBtrp plasmid yielded a large fraction (more than 50 percent) of mutants defective in some plasmid functions, including its own maintenance in the host bacteria. The results of preliminary characterization of strains carrying these mutated plasmids are presented."}
{"id": "PMID:1091633", "title": "Variation of ribosomal proteins with bacterial growth rate.", "content": "The composition of ribosomal proteins has been examined as a function of the growth rate of Escherichia coli cells. Seven sets of cultural conditions, utilizing different combinations of carbon and nitrogen sources, were employed to provide a 36-fold spread in growth rate. The cellular content of most of the ribosomal proteins in ribosomes decreased to a similar extent in the very slow-growing cultures. Major exceptions were proteins S6 and L12, which exhibited a much more pronounced decrease , and S21, which exhibited an increase. None of the proteins remained invariant with growth rate.", "contents": "Variation of ribosomal proteins with bacterial growth rate. The composition of ribosomal proteins has been examined as a function of the growth rate of Escherichia coli cells. Seven sets of cultural conditions, utilizing different combinations of carbon and nitrogen sources, were employed to provide a 36-fold spread in growth rate. The cellular content of most of the ribosomal proteins in ribosomes decreased to a similar extent in the very slow-growing cultures. Major exceptions were proteins S6 and L12, which exhibited a much more pronounced decrease , and S21, which exhibited an increase. None of the proteins remained invariant with growth rate."}
{"id": "PMID:1091634", "title": "Effects of colicin Ia on transport and respiration in Escherichia coli.", "content": "Treatment of Escherichia coli with colicin Ia leads to an inhibition in the active transport of exogenously supplied proline, thiomethyl-beta-D-galactoside and potassium ion. Furthermore, the addition of colicin Ia to cells preloaded with these substances leads to their almost immediate efflux. In contrast, colicin tia treatment enhances by as much as 10-fold the level of accumulation of alpha-methyl-D-glucoside. The colicin Ia-induced stimulation of glucoside accumulation is mediated by the phosphotransferase system. Cells treated with colicin Ia exhibit an increased rate of respiration when glucose is the substrate and a decreased rate when glycerol or succinate is the substrate; The decreased rate of succinate-dependent respiration is probably due to the failure of Ia-treated cells to accumulate succinate.", "contents": "Effects of colicin Ia on transport and respiration in Escherichia coli. Treatment of Escherichia coli with colicin Ia leads to an inhibition in the active transport of exogenously supplied proline, thiomethyl-beta-D-galactoside and potassium ion. Furthermore, the addition of colicin Ia to cells preloaded with these substances leads to their almost immediate efflux. In contrast, colicin tia treatment enhances by as much as 10-fold the level of accumulation of alpha-methyl-D-glucoside. The colicin Ia-induced stimulation of glucoside accumulation is mediated by the phosphotransferase system. Cells treated with colicin Ia exhibit an increased rate of respiration when glucose is the substrate and a decreased rate when glycerol or succinate is the substrate; The decreased rate of succinate-dependent respiration is probably due to the failure of Ia-treated cells to accumulate succinate."}
{"id": "PMID:1091635", "title": "Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092.", "content": "A protein which binds both glutamate (K-D = 0.8 muM) and aspartate (K-D = 1.2 muM) has been purified to homogeneity (290-fold) from the periplasmic fraction released from Escherichia coli W3092 by the cold osmotic shock procedure. The apparent molecular weight of the glutamate-aspartate binding protein is approximately 31,000 as judged by gel electrophoresis, gel filtration, and sedimentation equilibrium centrifugation; and the protein has a pI of 9.69. This protein contains 2 half-cystine residues and is dependent on a dithiothreitol-sensitive component for renaturation to an active conformation following urea or guanidine treatment. Of the natural amino acids only the L isomers of glutamate, aspartate, glutamine, asparagine, and alanine were inhibitors of either [C]glutamate or [14C]aspartate binding and the inhibitions were competitive. Only one binding site is indicated per molecule of protein. Antibody prepared against the glutamate-asparate binding protein does not cross-react with purified glutamine binding protein or any other component of osmotic shock fluid. The antibody does cross-react with osmotic shock fluids obtained from E. coli strains B and W and Salmonella typhimurium OT2. The glutamate-aspartate binding protein-antibody complex does not bind either glutamate or aspartate. The protein may be similar to the glutamate binding activity detected in the periplasmic fraction released from E. coli strain B (Miner, K.M., and Frank, L. (1974) J. Bacteriol. 117, 1093-1098) and strain K12 CS (Barash, H., and Halpern, Y.S. (1971) Biochem. Biophys. Res. Commun. 45, 681-688). This protein appears to function in the transport of glutamate by E. coli strain W cultured in minimal medium with succinate as the carbon source (Willis, R.C., and Furlong, C.E. (1975) J. Biol. Chem. 250, 2581-2586.", "contents": "Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092. A protein which binds both glutamate (K-D = 0.8 muM) and aspartate (K-D = 1.2 muM) has been purified to homogeneity (290-fold) from the periplasmic fraction released from Escherichia coli W3092 by the cold osmotic shock procedure. The apparent molecular weight of the glutamate-aspartate binding protein is approximately 31,000 as judged by gel electrophoresis, gel filtration, and sedimentation equilibrium centrifugation; and the protein has a pI of 9.69. This protein contains 2 half-cystine residues and is dependent on a dithiothreitol-sensitive component for renaturation to an active conformation following urea or guanidine treatment. Of the natural amino acids only the L isomers of glutamate, aspartate, glutamine, asparagine, and alanine were inhibitors of either [C]glutamate or [14C]aspartate binding and the inhibitions were competitive. Only one binding site is indicated per molecule of protein. Antibody prepared against the glutamate-asparate binding protein does not cross-react with purified glutamine binding protein or any other component of osmotic shock fluid. The antibody does cross-react with osmotic shock fluids obtained from E. coli strains B and W and Salmonella typhimurium OT2. The glutamate-aspartate binding protein-antibody complex does not bind either glutamate or aspartate. The protein may be similar to the glutamate binding activity detected in the periplasmic fraction released from E. coli strain B (Miner, K.M., and Frank, L. (1974) J. Bacteriol. 117, 1093-1098) and strain K12 CS (Barash, H., and Halpern, Y.S. (1971) Biochem. Biophys. Res. Commun. 45, 681-688). This protein appears to function in the transport of glutamate by E. coli strain W cultured in minimal medium with succinate as the carbon source (Willis, R.C., and Furlong, C.E. (1975) J. Biol. Chem. 250, 2581-2586."}
{"id": "PMID:1091636", "title": "Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli.", "content": "Escherichia coli cells cultured with succinate as the carbon source display apparent K-m values for the uptake of L-glutamate of 10 muM in the absence of added sodium ion and 0.7 muM in the presence of an optimal level of sodium ion (15 to 50 mM). The glutamate transport system of the  succinate cultured cells is noncompetitively inhibited by L-aspartate. A protein which binds glutamate and aspartate with K-D values of 0.7 and 1.2 muM, respectively, is released from the succinate cultured cells by osmotic shock or with the formation of spheroplasts during the preparation of membrane vesicles. The membrane vesicles of succinate cultured cells do not retain the whole cell capacity for L-glutamate uptake, but do retain much of the whole cell capacity for L-aspartate uptake. Culture of E. coli cells with glucose as carbon source causes a 2- to 3-fold repression of glutamate-aspartate binding protein but does not affect the velocity component of glutamate transport. As shown by other workers, the glutamate transport system of glucose cultured cells displays a sodium affected K-m value (FRANK, L., AND HOPKINS, I. (1969) J. Bacteriol. 100, 329-336) and is noncompetitively inhibited by L-aspartate (HALPERN, Y. S., AND EVEN-SHOSHAN, A. (1967) J. Bacteriol. 93, 1009-1016). Membrane vesicles prepared from glucose cultured cells retain the whole cell capacity for the uptake of glutamate (LOMBARDI, J. F., AND KABACK, H. R. (1972) J. Biol. Chem. 247, 7844-7857). The glutamate transport system of E. coli strain W appears to be conditionally dependent on the presence of the osmotic shock-releasable glutamate-aspartate binding protein. The results are interpreted to suggest that the binding protein-ligand complex acts as a substrate which is competitive with unbound substrate(s) for a sodium affected translocation process; the organization and specificity of which are dependent on the carbon source of the culture.", "contents": "Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli. Escherichia coli cells cultured with succinate as the carbon source display apparent K-m values for the uptake of L-glutamate of 10 muM in the absence of added sodium ion and 0.7 muM in the presence of an optimal level of sodium ion (15 to 50 mM). The glutamate transport system of the  succinate cultured cells is noncompetitively inhibited by L-aspartate. A protein which binds glutamate and aspartate with K-D values of 0.7 and 1.2 muM, respectively, is released from the succinate cultured cells by osmotic shock or with the formation of spheroplasts during the preparation of membrane vesicles. The membrane vesicles of succinate cultured cells do not retain the whole cell capacity for L-glutamate uptake, but do retain much of the whole cell capacity for L-aspartate uptake. Culture of E. coli cells with glucose as carbon source causes a 2- to 3-fold repression of glutamate-aspartate binding protein but does not affect the velocity component of glutamate transport. As shown by other workers, the glutamate transport system of glucose cultured cells displays a sodium affected K-m value (FRANK, L., AND HOPKINS, I. (1969) J. Bacteriol. 100, 329-336) and is noncompetitively inhibited by L-aspartate (HALPERN, Y. S., AND EVEN-SHOSHAN, A. (1967) J. Bacteriol. 93, 1009-1016). Membrane vesicles prepared from glucose cultured cells retain the whole cell capacity for the uptake of glutamate (LOMBARDI, J. F., AND KABACK, H. R. (1972) J. Biol. Chem. 247, 7844-7857). The glutamate transport system of E. coli strain W appears to be conditionally dependent on the presence of the osmotic shock-releasable glutamate-aspartate binding protein. The results are interpreted to suggest that the binding protein-ligand complex acts as a substrate which is competitive with unbound substrate(s) for a sodium affected translocation process; the organization and specificity of which are dependent on the carbon source of the culture."}
{"id": "PMID:1091637", "title": "Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.", "content": "Intracistronic alpha-complementation between a cyanogen bromide digest of beta-galactosidase and an extract of the lac Zminus operator-proximal deletion mutant M15 was used to monitor the purification of a cyanogen bromide peptide (CB2) responsible for the complementation. Key steps in the purification were ion exchange chromatography on carboxymethylcellulose and sulfopropyl-Sephadex in the presence of urea, and Sephadex gel filtration. CB2 contains residues 3 to 92 of beta-galactosidase. Its sequence is: Ile-Thr-Asp-Ser-Leu-Ala-Val-Val-Leu-Gln-Arg-Arg-Asp-Trp-Glu-Asn-Pro-Gly-Val-Thr-Gln-Leu-Asn-Arg-Leu-Ala-Ala-His-Pro-Pro-Phe-Ala-Ser-Trp-Arg-Asn-Ser-Glu-Glu-Ala-Arg-Thr-Asp-Arg-Pro-Ser-Gln-Gln-Leu-Arg-Ser-Leu-Asn-Gly-Glu-Trp-Arg-Phe-Ala-Trp-Phe-Pro-Ala-Pro-Glu-Ala-Val-Pro-Glu-Ser-Trp-Leu-Glu-Cys-Asp-Leu-Pro-Glu-Ala-Asp-Thr-Val-Val-Val-Pro-Ser-Asn-Trp-Gln-Met. Thus no more than 1/13 of the beta-galactosidase polypeptide chain, starting 2 residues from the NH2 terminus, is necessary for alpha-complementation with M15 as alpha-acceptor.", "contents": "Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92. Intracistronic alpha-complementation between a cyanogen bromide digest of beta-galactosidase and an extract of the lac Zminus operator-proximal deletion mutant M15 was used to monitor the purification of a cyanogen bromide peptide (CB2) responsible for the complementation. Key steps in the purification were ion exchange chromatography on carboxymethylcellulose and sulfopropyl-Sephadex in the presence of urea, and Sephadex gel filtration. CB2 contains residues 3 to 92 of beta-galactosidase. Its sequence is: Ile-Thr-Asp-Ser-Leu-Ala-Val-Val-Leu-Gln-Arg-Arg-Asp-Trp-Glu-Asn-Pro-Gly-Val-Thr-Gln-Leu-Asn-Arg-Leu-Ala-Ala-His-Pro-Pro-Phe-Ala-Ser-Trp-Arg-Asn-Ser-Glu-Glu-Ala-Arg-Thr-Asp-Arg-Pro-Ser-Gln-Gln-Leu-Arg-Ser-Leu-Asn-Gly-Glu-Trp-Arg-Phe-Ala-Trp-Phe-Pro-Ala-Pro-Glu-Ala-Val-Pro-Glu-Ser-Trp-Leu-Glu-Cys-Asp-Leu-Pro-Glu-Ala-Asp-Thr-Val-Val-Val-Pro-Ser-Asn-Trp-Gln-Met. Thus no more than 1/13 of the beta-galactosidase polypeptide chain, starting 2 residues from the NH2 terminus, is necessary for alpha-complementation with M15 as alpha-acceptor."}
{"id": "PMID:1091638", "title": "Purification and properties of rabbit reticulocyte protein synthesis elongation factor 2.", "content": "A homogeneous preparation of elongation factor 2 (EF-2) has been obtained from rabbit reticulocytes. EF-2, purified 1,960-fold, appears to be active as a single polypeptide chain with a molecular weight of approximately 100,000 based upon the following determinations: sodium dodecyl sulfate gel electrophoresis (95,000); sedimentation equilibrium centrifugation (112,000); gel filtration (97,000); ADP-ribosylation (103,000). The amino acid composition of rabbit reticulocyte EF-2 is almost identical with that of rat liver EF-2. The unknown amino acid in rat liver EF-2 which can be ADP-ribosylated appears also to be present in rabbit reticulocyte EF-2. A comparison of the amino acid composition of rabbit reticulocyte and rat liver EF-2 with Escherichia coli EF-G shows a high degree of similarity with only four amino acids differing by more than 10% (alanine, lysine, cysteine, and leucine).", "contents": "Purification and properties of rabbit reticulocyte protein synthesis elongation factor 2. A homogeneous preparation of elongation factor 2 (EF-2) has been obtained from rabbit reticulocytes. EF-2, purified 1,960-fold, appears to be active as a single polypeptide chain with a molecular weight of approximately 100,000 based upon the following determinations: sodium dodecyl sulfate gel electrophoresis (95,000); sedimentation equilibrium centrifugation (112,000); gel filtration (97,000); ADP-ribosylation (103,000). The amino acid composition of rabbit reticulocyte EF-2 is almost identical with that of rat liver EF-2. The unknown amino acid in rat liver EF-2 which can be ADP-ribosylated appears also to be present in rabbit reticulocyte EF-2. A comparison of the amino acid composition of rabbit reticulocyte and rat liver EF-2 with Escherichia coli EF-G shows a high degree of similarity with only four amino acids differing by more than 10% (alanine, lysine, cysteine, and leucine)."}
{"id": "PMID:1091639", "title": "Enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin II.", "content": "Porcine beta-melanocyte-stimulating hormone and angiotensin II were examined as acceptors in the reaction catalyzed by arginyl-tRNA-protein transferase. Both inhibited enzymatic transfer of [14C]arginine from tRNA to bovine albumin. Inhibition was competitive with albumin and the K-i values were, respectively, 15 and 0.8 muM. The expected arginylated compounds were isolated and characterized. Beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epithelium bioassay. In contrast, the biological activity of angiotensin II was diminished by enzymatic arginylation. The pressor effect of the arginylated derivative on anesthetized rats and its activity on the isolated rat uterus were, respectively, approximately 60% and 20% of those found for the unmodified peptide.", "contents": "Enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin II. Porcine beta-melanocyte-stimulating hormone and angiotensin II were examined as acceptors in the reaction catalyzed by arginyl-tRNA-protein transferase. Both inhibited enzymatic transfer of [14C]arginine from tRNA to bovine albumin. Inhibition was competitive with albumin and the K-i values were, respectively, 15 and 0.8 muM. The expected arginylated compounds were isolated and characterized. Beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epithelium bioassay. In contrast, the biological activity of angiotensin II was diminished by enzymatic arginylation. The pressor effect of the arginylated derivative on anesthetized rats and its activity on the isolated rat uterus were, respectively, approximately 60% and 20% of those found for the unmodified peptide."}
{"id": "PMID:1091641", "title": "Coupling of alanine racemase and D-alanine dehydrogenase to active transport of amino acids in Escherichia coli B membrane vesicles.", "content": "Isolated membrane vesicles from Escherichia coli B grown on DL-alanine-glycerol carry out amino acid active transport coupled to D-alanine oxidation by a membrane-bound dehydrogenase. Several other D-amino acids are substrates for this D-alanine dehydrogenase and also drive concentrative uptake of solutes. Additionally, L-alanine and L-serine can energize solute transport by virtue of conversion to oxidizable D isomers by a membrane-bound alanine racemase. No other physiological L-amino acids were effective. Both membrane enzymes and consequent solute transport are markedly reduced in vesicles from glucose-grown cells. Respiratory chain uncouplers abolish the racemase-dehydrogenase-supported transport activity. When amino-oxyacetate at 10-4 M is added to the vesicles, the racemase activity and transport driven by L-alanine and L-serine is specifically and reversibly inhibited. D-Alanine-driven transport is unaffected. Similarly beta-chloro-L-alanine is an irreversible inactivator of the bound racemase but not the D-alanine dehydrogenase. Both the D and L isomers of beta-chloroalanine support oxygen uptake by the vesicles and initially stimulate L-(14C)proline active transport. However, oxidation of the beta-chloro-D-alanine rapidly uncouples active transport from substrate oxidation. This transport inactivation can be protected partially by dithiothreitol, putatively scavenging a reactive product of chloroalanine oxidation. Authentic beta-chloropyruvate produces the same transport uncoupling. When beta-chloro-L-alanine is employed as a substrate, no such transport inactivation is observed. This difference may stem from the possibility that the alanine racemase eliminates HCl from beta-chloro-L-alanine producing pyruvate, not the beta-chloropyruvate that would arise from racemization and then dehydrogenation. We have shown that exogenous pyruvate is oxidized by the vesicles and will also stimulate active transport of amino acids.", "contents": "Coupling of alanine racemase and D-alanine dehydrogenase to active transport of amino acids in Escherichia coli B membrane vesicles. Isolated membrane vesicles from Escherichia coli B grown on DL-alanine-glycerol carry out amino acid active transport coupled to D-alanine oxidation by a membrane-bound dehydrogenase. Several other D-amino acids are substrates for this D-alanine dehydrogenase and also drive concentrative uptake of solutes. Additionally, L-alanine and L-serine can energize solute transport by virtue of conversion to oxidizable D isomers by a membrane-bound alanine racemase. No other physiological L-amino acids were effective. Both membrane enzymes and consequent solute transport are markedly reduced in vesicles from glucose-grown cells. Respiratory chain uncouplers abolish the racemase-dehydrogenase-supported transport activity. When amino-oxyacetate at 10-4 M is added to the vesicles, the racemase activity and transport driven by L-alanine and L-serine is specifically and reversibly inhibited. D-Alanine-driven transport is unaffected. Similarly beta-chloro-L-alanine is an irreversible inactivator of the bound racemase but not the D-alanine dehydrogenase. Both the D and L isomers of beta-chloroalanine support oxygen uptake by the vesicles and initially stimulate L-(14C)proline active transport. However, oxidation of the beta-chloro-D-alanine rapidly uncouples active transport from substrate oxidation. This transport inactivation can be protected partially by dithiothreitol, putatively scavenging a reactive product of chloroalanine oxidation. Authentic beta-chloropyruvate produces the same transport uncoupling. When beta-chloro-L-alanine is employed as a substrate, no such transport inactivation is observed. This difference may stem from the possibility that the alanine racemase eliminates HCl from beta-chloro-L-alanine producing pyruvate, not the beta-chloropyruvate that would arise from racemization and then dehydrogenation. We have shown that exogenous pyruvate is oxidized by the vesicles and will also stimulate active transport of amino acids."}
{"id": "PMID:1091642", "title": "Transcription of bacteriophage deoxyribonucleic acid. Comparison of Escherichia coli and Azotobacter vinelandii sigma subunits.", "content": "The effect of the sigma subunit of RNA polymerase on the rate and asymmetry of the in vitro transcription of Escherichia coli and Azotobacter vinelandii phage DNAs has been studied with purified E. coli and A. vinelandii RNA polymerases and hybrid enzymes containing the core subunits of one enzyme and sigma from the other. The effect of sigma on the rate of transcription is characteristic of the template and not of the enzyme and depends on ionic strength. The rate of transcription of A. vinelandii phage A21 DNA is decreased by sigma at high ionic strength, but shows the more characteristic stimulation at KCl concentrations below 0.05 M. In contrast, the stimulation by sigma of T4 DNA transcription increased with an increase in the KCl concentrations. All combinations of core and sigma subunits behaved similarly with respect to stimulation or inhibition by sigma and with respect to asymmetric transcription of S13 replicative form (RF)DNA. However, the heterologous, but not the homologous combinations of core and sigma transcribed A21 symmetrically. S13 RF DNA in the superhelical, but not in the relaxed configuration, is transcribed asymmetrically by the A. vinelandii core enzyme. A role for the core subunits in specific site recognition is indicated by this observation.", "contents": "Transcription of bacteriophage deoxyribonucleic acid. Comparison of Escherichia coli and Azotobacter vinelandii sigma subunits. The effect of the sigma subunit of RNA polymerase on the rate and asymmetry of the in vitro transcription of Escherichia coli and Azotobacter vinelandii phage DNAs has been studied with purified E. coli and A. vinelandii RNA polymerases and hybrid enzymes containing the core subunits of one enzyme and sigma from the other. The effect of sigma on the rate of transcription is characteristic of the template and not of the enzyme and depends on ionic strength. The rate of transcription of A. vinelandii phage A21 DNA is decreased by sigma at high ionic strength, but shows the more characteristic stimulation at KCl concentrations below 0.05 M. In contrast, the stimulation by sigma of T4 DNA transcription increased with an increase in the KCl concentrations. All combinations of core and sigma subunits behaved similarly with respect to stimulation or inhibition by sigma and with respect to asymmetric transcription of S13 replicative form (RF)DNA. However, the heterologous, but not the homologous combinations of core and sigma transcribed A21 symmetrically. S13 RF DNA in the superhelical, but not in the relaxed configuration, is transcribed asymmetrically by the A. vinelandii core enzyme. A role for the core subunits in specific site recognition is indicated by this observation."}
{"id": "PMID:1091643", "title": "Transcription of Azotobacter phage deoxyribonucleic acid. Salt-dependent equilibrium between steps in initiation.", "content": "The transcription of Azotobacter phage A21 DNA by Escherichia coli or Azotobacter vinelandii RNA polymerase differs from that of some other DNAs in its inhibition by moderate concentrations of KCl. This characteristic results in an apparent low template activity for this DNA as compared with T4 DNA under standard assay conditions. From an analysis of the dependence of the various steps in initiation on KCl it is concluded that the effect is exerted on an equilibrium between an inactive polymerase-DNA complex and an active preintitiation complex. This salt-sensitive equilibrium favors the inactive complex at a lower KCl concentration than with other templates. It can be approached from other low or high salt concentrations at a measurably slow rate.", "contents": "Transcription of Azotobacter phage deoxyribonucleic acid. Salt-dependent equilibrium between steps in initiation. The transcription of Azotobacter phage A21 DNA by Escherichia coli or Azotobacter vinelandii RNA polymerase differs from that of some other DNAs in its inhibition by moderate concentrations of KCl. This characteristic results in an apparent low template activity for this DNA as compared with T4 DNA under standard assay conditions. From an analysis of the dependence of the various steps in initiation on KCl it is concluded that the effect is exerted on an equilibrium between an inactive polymerase-DNA complex and an active preintitiation complex. This salt-sensitive equilibrium favors the inactive complex at a lower KCl concentration than with other templates. It can be approached from other low or high salt concentrations at a measurably slow rate."}
{"id": "PMID:1091644", "title": "Ribonucleic acid processing activity of Escherichia coli ribonuclease III.", "content": "We have studied the nuclease activities present in preparations of Escherichia coli RNase III and the \"sizing factor\" responsible for specific processing of several RNA species. RNase III preparations contain three activities: one which solubilizes stable RNA:RNA duplexes; one which solubilizes the RNA of DNA:RNA hybrids; and one which processes the polycistronic mRNA of bacteriophage T7 in a manner identical with sizing factor. We show that the activity against the  RNA of DNA:RNA hybrids can be removed, but that the activity which cleaves RNA:RNA duplexes and that responsible for specific processing of phage T7 polycistronic mRNA appear to be identical by several biochemical criteria. In addition, partially purified enzyme fractions from mutants lacking these two activities contain substantial amounts of activity against the RNA of DNA:RNA hybrids. We have also defined several properties of the two activities solubilize RNA:RNA duplexes and RNA of DNA:RNA hybrids. Average oligonucleotide chain length in an exhaustive digest of double-stranded RNA is about 15 bases, while that in a digest of the RNA in DNA:RNA hybrids is less than 10 bases. Direct analysis shows that both activities cleave RNA chains to yield 5'-phosphate and 3'-hydroxyl termini. All four bases can reside at the 5' end of the resulting oligonucleotides, although both activities show a mild preference for certain bases. These results and previous findings allow us to specify the probably size and structure of potential cleavage sites for these enzymes in biological RNA molecules.", "contents": "Ribonucleic acid processing activity of Escherichia coli ribonuclease III. We have studied the nuclease activities present in preparations of Escherichia coli RNase III and the \"sizing factor\" responsible for specific processing of several RNA species. RNase III preparations contain three activities: one which solubilizes stable RNA:RNA duplexes; one which solubilizes the RNA of DNA:RNA hybrids; and one which processes the polycistronic mRNA of bacteriophage T7 in a manner identical with sizing factor. We show that the activity against the  RNA of DNA:RNA hybrids can be removed, but that the activity which cleaves RNA:RNA duplexes and that responsible for specific processing of phage T7 polycistronic mRNA appear to be identical by several biochemical criteria. In addition, partially purified enzyme fractions from mutants lacking these two activities contain substantial amounts of activity against the RNA of DNA:RNA hybrids. We have also defined several properties of the two activities solubilize RNA:RNA duplexes and RNA of DNA:RNA hybrids. Average oligonucleotide chain length in an exhaustive digest of double-stranded RNA is about 15 bases, while that in a digest of the RNA in DNA:RNA hybrids is less than 10 bases. Direct analysis shows that both activities cleave RNA chains to yield 5'-phosphate and 3'-hydroxyl termini. All four bases can reside at the 5' end of the resulting oligonucleotides, although both activities show a mild preference for certain bases. These results and previous findings allow us to specify the probably size and structure of potential cleavage sites for these enzymes in biological RNA molecules."}
{"id": "PMID:1091645", "title": "Synthesis and turnover of ribosomal ribonucleic acid in guanine-starved cells of Escherichia coli.", "content": "The relationship between the rate of RNA accumulation and the level of guanosine triphosphate was examined. Cells auxotrophic for guanine show a 6-fold drop in the intracellular level of GTP in response to the exhaustion of the exogenous guanosine supply. Contraction of the GTP pool results in a 2.5-fold reduction in the rate of RNA synthesis and the cessation of RNA accumulation. The decrease in the rate of RNA synthesis is seen to occur at the level of chain elongation. Analysis of RNA made in guanine-starved cells by competition-hybridization and sucrose gradient sedimentation suggests that the turnover of newly synthesized ribosomal RNA accounts for the observed failure of RNA to accumulate.", "contents": "Synthesis and turnover of ribosomal ribonucleic acid in guanine-starved cells of Escherichia coli. The relationship between the rate of RNA accumulation and the level of guanosine triphosphate was examined. Cells auxotrophic for guanine show a 6-fold drop in the intracellular level of GTP in response to the exhaustion of the exogenous guanosine supply. Contraction of the GTP pool results in a 2.5-fold reduction in the rate of RNA synthesis and the cessation of RNA accumulation. The decrease in the rate of RNA synthesis is seen to occur at the level of chain elongation. Analysis of RNA made in guanine-starved cells by competition-hybridization and sucrose gradient sedimentation suggests that the turnover of newly synthesized ribosomal RNA accounts for the observed failure of RNA to accumulate."}
{"id": "PMID:1091646", "title": "Comparison of fMet-tRNAf and Met-tRNAf from Escherichia coli and rabbit liver in initiation of hemoglobin synthesis.", "content": "A comparison has been made of the ability of the formylated and unformylated initiator tRNAs of Escherichia coli and rabbit liver to participate in a number of model reactions of protein synthesis. These reactions include: (a) formation of a ternary complex composed of the initiator tRNA, GTP, and initiation factor MP; (b) ApUpG-directed binding of the initiator tRNA to 40 S subunits with initiation factor Ml; (c) formation of the artificial dipeptide, methionylpuromycin; (d) formation of the natural initial globin dipeptide, methionylvaline; and (e) synthesis of sheep alpha and betaB-globin chains on reticulocyte polysomes from a type BB sheep. The results of these studies indicate that although the prokaryotic initiator tRNA species function efficiently in the partial reactions which involve only binding, the methionine donated by the prokaryotic tRNA is not incorporated efficiently into peptide linkage. This suggests that the initial high level of binding of the E. coli initiator tRNAs may be nonspecific, and that the structure of the tRNA itself is important for specific recognition by eukaryotic initiation factors. The effect of formylation on the effectiveness of the initiator tRNA is not clear; it reduces activity in ternary complex formation, does not affect ApUpG-directed binding to 40 S subunits, and increases the rate or extent of incorporation of methionine, or both, into methionylpuromycin and globin chains.", "contents": "Comparison of fMet-tRNAf and Met-tRNAf from Escherichia coli and rabbit liver in initiation of hemoglobin synthesis. A comparison has been made of the ability of the formylated and unformylated initiator tRNAs of Escherichia coli and rabbit liver to participate in a number of model reactions of protein synthesis. These reactions include: (a) formation of a ternary complex composed of the initiator tRNA, GTP, and initiation factor MP; (b) ApUpG-directed binding of the initiator tRNA to 40 S subunits with initiation factor Ml; (c) formation of the artificial dipeptide, methionylpuromycin; (d) formation of the natural initial globin dipeptide, methionylvaline; and (e) synthesis of sheep alpha and betaB-globin chains on reticulocyte polysomes from a type BB sheep. The results of these studies indicate that although the prokaryotic initiator tRNA species function efficiently in the partial reactions which involve only binding, the methionine donated by the prokaryotic tRNA is not incorporated efficiently into peptide linkage. This suggests that the initial high level of binding of the E. coli initiator tRNAs may be nonspecific, and that the structure of the tRNA itself is important for specific recognition by eukaryotic initiation factors. The effect of formylation on the effectiveness of the initiator tRNA is not clear; it reduces activity in ternary complex formation, does not affect ApUpG-directed binding to 40 S subunits, and increases the rate or extent of incorporation of methionine, or both, into methionylpuromycin and globin chains."}
{"id": "PMID:1091647", "title": "Stoichiometry of compounds bound to human erythrocytes in relation to morphology.", "content": "Most work on human erythrocyte interaction with drugs and other compounds has been reported on the basis of total concentrations. Total concentrations alone do not reveal numbers of molecules bound per cell, v. This paper emphasizes determination of v and of binding isotherms, in conjunction with changes in cell morphologies and in hypotonic shock behavior as v is varied. Four drugs and five other compounds were studied, with fresh erythrocytes. The principal findings are: (1) the intact erythrocyte engages in two kinds of binding mechanisms, statistical binding and cooperative binding, depending on the compound. In the case of a detergent, dodecylbenzene sulfonate, the binding is nearly quantitative. (2) The compounds often induce considerable protection against hypotonic hemolysis. However, the binding levels at which maximum protection occurs are rather close to the levels, vL, that occur upon complete conversion to the first distorted morphology. Therefore, the maximally protected erythrocyte may be a distorted erythrocyte. (3) The value n is the apparent total number of sites from Scatchard plotting for compounds which bind in a statistical manner. Levels vp and vw characterize maxima in cooperative binding behavior, also from Scatchard plotting of the data. Despite the wide diversity of over-all levels at which compounds exert their effects, the critical binding levels of and numbers of sites fall into a narrow range:n, vL, vP, and vw are all between 1 and 8 times 10-7 molecules or sites per cell. Most of our data, and that from some other laboratories, indicate that about 2 plus and minus 1 times 10-7 sites per erythrocyte are available for compound binding by the intact cell. Beyond that level, the cell in suspension almost always will be forced into the first obvious morphology change, as seen by phase contrast microscopy. (4) Once stoichiometries are established, the total binding capacity of erythrocytes for such compounds, in blood, can be estimated. An intruding organic molecule would encounter about 6 times as many plasma albumin sites as erythrocyte sites, if the plasma albumin sites were free. However, because albumin in vivo usually forms a complex with one to two fatty acids, the erythrocyte itself is rather likely to act as a transport particle for such compounds.", "contents": "Stoichiometry of compounds bound to human erythrocytes in relation to morphology. Most work on human erythrocyte interaction with drugs and other compounds has been reported on the basis of total concentrations. Total concentrations alone do not reveal numbers of molecules bound per cell, v. This paper emphasizes determination of v and of binding isotherms, in conjunction with changes in cell morphologies and in hypotonic shock behavior as v is varied. Four drugs and five other compounds were studied, with fresh erythrocytes. The principal findings are: (1) the intact erythrocyte engages in two kinds of binding mechanisms, statistical binding and cooperative binding, depending on the compound. In the case of a detergent, dodecylbenzene sulfonate, the binding is nearly quantitative. (2) The compounds often induce considerable protection against hypotonic hemolysis. However, the binding levels at which maximum protection occurs are rather close to the levels, vL, that occur upon complete conversion to the first distorted morphology. Therefore, the maximally protected erythrocyte may be a distorted erythrocyte. (3) The value n is the apparent total number of sites from Scatchard plotting for compounds which bind in a statistical manner. Levels vp and vw characterize maxima in cooperative binding behavior, also from Scatchard plotting of the data. Despite the wide diversity of over-all levels at which compounds exert their effects, the critical binding levels of and numbers of sites fall into a narrow range:n, vL, vP, and vw are all between 1 and 8 times 10-7 molecules or sites per cell. Most of our data, and that from some other laboratories, indicate that about 2 plus and minus 1 times 10-7 sites per erythrocyte are available for compound binding by the intact cell. Beyond that level, the cell in suspension almost always will be forced into the first obvious morphology change, as seen by phase contrast microscopy. (4) Once stoichiometries are established, the total binding capacity of erythrocytes for such compounds, in blood, can be estimated. An intruding organic molecule would encounter about 6 times as many plasma albumin sites as erythrocyte sites, if the plasma albumin sites were free. However, because albumin in vivo usually forms a complex with one to two fatty acids, the erythrocyte itself is rather likely to act as a transport particle for such compounds."}
{"id": "PMID:1091648", "title": "Decay of ribosomal ribonucleic acid in Escherichia coli cells starved for various nutrients.", "content": "Decay of pre-existing ribonucleic acid was studied in Escherichia coli cells subjected to high temperature or to starvation for nitrogen, phosphate, amino acids, or a carbon source. In these studies a series of mutants affected in ribonucleic I(RNase I, EC 3.1.4.22) polynucleotide phosphorylase (EC 2.7.7.8) or ribonuclease II (RNase II, EC 3.1.4.23) were used. Degradation of total RNA and the disappearance of 23 S and 16 S rRNA were followed. The results obtained indicated that, by and large, decay of 23 S and 16 S RNA parallels that of total RNA. Decay of RNA depended on the nuclease content of the cells as well as on the treatment of applied. It was most pronounced during carbon starvation and least in cells deprived of phosphate ions. It was most effective in strains containing all three nucleases and least in the strain defective in all three. The exonucleases polynucleotide phosphorylase and RNase II did not seem to affect the extent of 23 S and 16 S RNA disappearance. Strains with modified exonucleases did accumulate low molecular weight RNA species during treatments which induced considerable degradation of 23 S and 16 S RNA. Based on the above date and previous observations, we suggest that during various starvations a similar mechanism is operative. The 23 S and 16 S RNAs are degraded endonucleolytically, and this is the rate-limiting step during starvation. The exonucleases  polynucleotide phosphorylase and RNase II seem to participate primarily in the decay of the low molecular weight RNA species formed by the endonuclease(s), not as yet identified.", "contents": "Decay of ribosomal ribonucleic acid in Escherichia coli cells starved for various nutrients. Decay of pre-existing ribonucleic acid was studied in Escherichia coli cells subjected to high temperature or to starvation for nitrogen, phosphate, amino acids, or a carbon source. In these studies a series of mutants affected in ribonucleic I(RNase I, EC 3.1.4.22) polynucleotide phosphorylase (EC 2.7.7.8) or ribonuclease II (RNase II, EC 3.1.4.23) were used. Degradation of total RNA and the disappearance of 23 S and 16 S rRNA were followed. The results obtained indicated that, by and large, decay of 23 S and 16 S RNA parallels that of total RNA. Decay of RNA depended on the nuclease content of the cells as well as on the treatment of applied. It was most pronounced during carbon starvation and least in cells deprived of phosphate ions. It was most effective in strains containing all three nucleases and least in the strain defective in all three. The exonucleases polynucleotide phosphorylase and RNase II did not seem to affect the extent of 23 S and 16 S RNA disappearance. Strains with modified exonucleases did accumulate low molecular weight RNA species during treatments which induced considerable degradation of 23 S and 16 S RNA. Based on the above date and previous observations, we suggest that during various starvations a similar mechanism is operative. The 23 S and 16 S RNAs are degraded endonucleolytically, and this is the rate-limiting step during starvation. The exonucleases  polynucleotide phosphorylase and RNase II seem to participate primarily in the decay of the low molecular weight RNA species formed by the endonuclease(s), not as yet identified."}
{"id": "PMID:1091650", "title": "Primary structure of the L chain from a rabbit homogeneous antibody to streptococcal carbohydrate. II. Sequence determination of peptides from tryptic and peptic digests.", "content": "A complete amino acid sequence containing 210 residues of a rabbit light chain of a homogeneous Group C streptococcal antibody has been determined as: See journal for formula. This light chain is allotype b4 and is of the V-K1 subgroup.", "contents": "Primary structure of the L chain from a rabbit homogeneous antibody to streptococcal carbohydrate. II. Sequence determination of peptides from tryptic and peptic digests. A complete amino acid sequence containing 210 residues of a rabbit light chain of a homogeneous Group C streptococcal antibody has been determined as: See journal for formula. This light chain is allotype b4 and is of the V-K1 subgroup."}
{"id": "PMID:1091651", "title": "Kinetic and equilibrium studies on the activation of Escherichia coli K12 tryptophanase by pyridoxal 5'-phosphate and monovalent cations.", "content": "An improved purification of Escherichia coli K12 tryptophanase is presented. It is shown that the apoenzyme crystals, oxidized by exposure to air, can be reactivated by treatment with a reducing agent. The titration of sulfhydryl groups shows that four --SH groups are exposed and two are masked per protomer. The influence of two effectors, monovalent cations and the coenzyme pyridoxal 5'-phosphate, on the reactivity of --SH groups and the enzymatic activity was investigated. The --SH groups react more slowly in holo- than in apoenzyme in the presence of potassium ions. If these ions are replaced by sodium ions, the reactivity becomes the same. Potassium and ammonium ions, both activators, give sigmoidal activation curves. The sodium ion is a Michaelian inhibitor of potassium activation. The binding of pyridoxal 5'-phosphate was examined by kinetics and at equilibrium. The kinetics are shown to be very slow; the rate constants of the forward and reverse reactions have been measured. The binding equilibrium, examined with 3H-labeled pyridoxal 5'-phosphate, gives one site per protomer with a K-D value of (3.2 plus or minus 0.8) times 10-7 M. The K-m for pyridoxal-P was determined by activity measurements. The binding equilibrium is attained after several hours, giving a value of 4.2 times 10-7 M, being nearly identical with the dissociation constant and 5 times smaller than previously reported.", "contents": "Kinetic and equilibrium studies on the activation of Escherichia coli K12 tryptophanase by pyridoxal 5'-phosphate and monovalent cations. An improved purification of Escherichia coli K12 tryptophanase is presented. It is shown that the apoenzyme crystals, oxidized by exposure to air, can be reactivated by treatment with a reducing agent. The titration of sulfhydryl groups shows that four --SH groups are exposed and two are masked per protomer. The influence of two effectors, monovalent cations and the coenzyme pyridoxal 5'-phosphate, on the reactivity of --SH groups and the enzymatic activity was investigated. The --SH groups react more slowly in holo- than in apoenzyme in the presence of potassium ions. If these ions are replaced by sodium ions, the reactivity becomes the same. Potassium and ammonium ions, both activators, give sigmoidal activation curves. The sodium ion is a Michaelian inhibitor of potassium activation. The binding of pyridoxal 5'-phosphate was examined by kinetics and at equilibrium. The kinetics are shown to be very slow; the rate constants of the forward and reverse reactions have been measured. The binding equilibrium, examined with 3H-labeled pyridoxal 5'-phosphate, gives one site per protomer with a K-D value of (3.2 plus or minus 0.8) times 10-7 M. The K-m for pyridoxal-P was determined by activity measurements. The binding equilibrium is attained after several hours, giving a value of 4.2 times 10-7 M, being nearly identical with the dissociation constant and 5 times smaller than previously reported."}
{"id": "PMID:1091652", "title": "Basement membrane collagen of renal glomerulus.", "content": "Collagen has been prepared from steer glomerular basement membrane by controlled pepsin solubilization. Four collagen polypeptides of potential alpha chain size have been isolated following denaturation and reduction with mercaptoethanol. Purification was obtained through sequential chromatography by gel filtration on agarose and by ion exchange on carboxymethyl- and diethylaminoethylcellulose and confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fractions A and B resemble interstitial alpha chains in apparent molecular weight by gel filtration (93,000). Fractions C and D were released from a single high molecular weight fraction (II) by reduction with mercaptoethanol and had a larger apparent molecular weight by gel filtration (140,000). Amino acid composition of all fractions demonstrated that they are closely interrelated by generally distinctive from interstitial collagens. Cysteine was present in all fractions except Fraction A. Prior to reduction, all mercaptan groups were inaccessible to iodoacetate and p-chloromercuribenzoate but became completely titrable after treatment with mercaptoethanol. The starting material and all fractions contained large amounts of hexose. Glucose and galactose predominated; but mannose, glucosamine, and galactosamine were also present in substantial amounts. Small amounts of fucose and sialic acids were found in starting material only.", "contents": "Basement membrane collagen of renal glomerulus. Collagen has been prepared from steer glomerular basement membrane by controlled pepsin solubilization. Four collagen polypeptides of potential alpha chain size have been isolated following denaturation and reduction with mercaptoethanol. Purification was obtained through sequential chromatography by gel filtration on agarose and by ion exchange on carboxymethyl- and diethylaminoethylcellulose and confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fractions A and B resemble interstitial alpha chains in apparent molecular weight by gel filtration (93,000). Fractions C and D were released from a single high molecular weight fraction (II) by reduction with mercaptoethanol and had a larger apparent molecular weight by gel filtration (140,000). Amino acid composition of all fractions demonstrated that they are closely interrelated by generally distinctive from interstitial collagens. Cysteine was present in all fractions except Fraction A. Prior to reduction, all mercaptan groups were inaccessible to iodoacetate and p-chloromercuribenzoate but became completely titrable after treatment with mercaptoethanol. The starting material and all fractions contained large amounts of hexose. Glucose and galactose predominated; but mannose, glucosamine, and galactosamine were also present in substantial amounts. Small amounts of fucose and sialic acids were found in starting material only."}
{"id": "PMID:1091653", "title": "Compression-plate fixation in acute diaphyseal fractures of the radius and ulna.", "content": "At the Campbell Clinic and City of Memphis Hospital from 1960 to 1970, 244 patients (216 with closed and twenty-eight with open fractures) had 330 acute diaphyseal fractures of the radius and ulna which were treated with ASIF compression plates and followed for from four months to nine years. One hundred and twelve patients had fractures of both bones of the forearm; fifty, single fractures of the ulna; and eighty-two, single fractures of the radius. In all, 193 fractures of the radius and 137 fractures of the ulna were treated by compression plating. Sixty-three patients (25.9 per cent) with severely comminuted fractures also had iliac-bone grafts. The over-all rate of union for the radius was 97.9 per cent and for the ulna, 96.3 per cent. ASIF compression plates, therefore, provided a successful method for obtaining union and restoring optimum function after acute diaphyseal fractures of the forearm.", "contents": "Compression-plate fixation in acute diaphyseal fractures of the radius and ulna. At the Campbell Clinic and City of Memphis Hospital from 1960 to 1970, 244 patients (216 with closed and twenty-eight with open fractures) had 330 acute diaphyseal fractures of the radius and ulna which were treated with ASIF compression plates and followed for from four months to nine years. One hundred and twelve patients had fractures of both bones of the forearm; fifty, single fractures of the ulna; and eighty-two, single fractures of the radius. In all, 193 fractures of the radius and 137 fractures of the ulna were treated by compression plating. Sixty-three patients (25.9 per cent) with severely comminuted fractures also had iliac-bone grafts. The over-all rate of union for the radius was 97.9 per cent and for the ulna, 96.3 per cent. ASIF compression plates, therefore, provided a successful method for obtaining union and restoring optimum function after acute diaphyseal fractures of the forearm."}
{"id": "PMID:1091654", "title": "Surgical treatment of the symptomatic unstable cervical spine in rheumatoid arthritis.", "content": "In rheumatoid arthritis spontaneous subluxation of cervical vertebrae is not infrequent and can usually be managed by conservative treatment. Surgical treatment, however, is necessary when there is neural involvement. Of twelve patients who had spine fusion for atlanto-axial subluxation only six had solid union. When conservative treatment fails, reduction by halo traction and arthrodesis of the cervical spine is the method recommended, but the best method of post-operative care remains uncertain. In these patients postoperative immobilization was provided by various methods, including a halo cast or brace, but no method appeared to be superior.", "contents": "Surgical treatment of the symptomatic unstable cervical spine in rheumatoid arthritis. In rheumatoid arthritis spontaneous subluxation of cervical vertebrae is not infrequent and can usually be managed by conservative treatment. Surgical treatment, however, is necessary when there is neural involvement. Of twelve patients who had spine fusion for atlanto-axial subluxation only six had solid union. When conservative treatment fails, reduction by halo traction and arthrodesis of the cervical spine is the method recommended, but the best method of post-operative care remains uncertain. In these patients postoperative immobilization was provided by various methods, including a halo cast or brace, but no method appeared to be superior."}
{"id": "PMID:1091656", "title": "Genetic expression of aryl hydrocarbon hydroxylase activity in the mouse.", "content": "Monooxygenases require NADPH and molecular oxygen during the metabolism of numerous endogenous hydrophobic substrates and carcinogenic and toxic exogenous chemicals. The complexity of these membrane-bound multicomponent drug-metabolizing enzyme systems is reviewed. What \"aryl hydrocarbon (benzo[a]pyrene) hydroxylase activity\" actually represents is reviewed and discussed. At least two forms of the hydroxylase activity exist and we suggest that they are associated with different molecular species of membrane-bound CO-binding hemoprotein (i.e., they are associated with different enzyme active-sties). At least two, and probably more than two, nonlinked loci are responsible for the genetic expression of new cytochrome P1450 formation and aryl hydrocarbon hydroxylase induction--and the stimulation of 10 other monooxygenase \"activities\"--in the mouse treated with certain aromatic hydrocarbons. The individual variability of hydroxylase activity in an inbred and in a random-bred strain of micr is illustrated. The basal hydroxylase activity appears to be inherited differently from the aromatic hydrocarbon-inducible hydroxylase activity. The potent inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin can stimulate increases in these hepatic monooxygenase activities and p1450 formation in so-called \"nonresponsive\" mice, whereas inducers such as beta-naphthoflavone and 3-methylcholanthrene cannot. Thus, the genetically \"nonresponsive\" micr apparently possess the structural and regulatory genes necessary for expression of these inducible monooxygenase activities and associated new formation of cytochrome P1450. We suggest that a mutation has occurred in the \"nonresponsive\" inbred strains that results in production of an inducer-binding receptor having a diminished affinity for aromatic hydrocarbons.", "contents": "Genetic expression of aryl hydrocarbon hydroxylase activity in the mouse. Monooxygenases require NADPH and molecular oxygen during the metabolism of numerous endogenous hydrophobic substrates and carcinogenic and toxic exogenous chemicals. The complexity of these membrane-bound multicomponent drug-metabolizing enzyme systems is reviewed. What \"aryl hydrocarbon (benzo[a]pyrene) hydroxylase activity\" actually represents is reviewed and discussed. At least two forms of the hydroxylase activity exist and we suggest that they are associated with different molecular species of membrane-bound CO-binding hemoprotein (i.e., they are associated with different enzyme active-sties). At least two, and probably more than two, nonlinked loci are responsible for the genetic expression of new cytochrome P1450 formation and aryl hydrocarbon hydroxylase induction--and the stimulation of 10 other monooxygenase \"activities\"--in the mouse treated with certain aromatic hydrocarbons. The individual variability of hydroxylase activity in an inbred and in a random-bred strain of micr is illustrated. The basal hydroxylase activity appears to be inherited differently from the aromatic hydrocarbon-inducible hydroxylase activity. The potent inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin can stimulate increases in these hepatic monooxygenase activities and p1450 formation in so-called \"nonresponsive\" mice, whereas inducers such as beta-naphthoflavone and 3-methylcholanthrene cannot. Thus, the genetically \"nonresponsive\" micr apparently possess the structural and regulatory genes necessary for expression of these inducible monooxygenase activities and associated new formation of cytochrome P1450. We suggest that a mutation has occurred in the \"nonresponsive\" inbred strains that results in production of an inducer-binding receptor having a diminished affinity for aromatic hydrocarbons."}
{"id": "PMID:1091657", "title": "Human T-cell heterogeneity as delineated with a specific human thymus lymphocyte antiserum. In vitro effects on mitogen response mixed leukocyte culture, cell-mediated lymphocytotoxicity, and lymphokine production.", "content": "Human peripheral blood lymphocytes (PBL) were evaluated by their responses to phytohemmagglutinin (PHA-P), concanavallin A (con-A), and pokeweed mitogen (PWM), both before and after treatment with an antiserum against human thymic lymphocyte antigens (HTLA) that had been made T-cell-specific by multiple absorptions with immunoglobulin EAC-positive lymphoblast cell lines (B cells). Cells treated with HTLA were examined for their ability to react in a mixed lymphocyte culture (MLC) and to form killer cells in a cell-mediated lymphocytotoxicity (CML) system. Sensitized cells were also examined for their ability to respond to purified protein derivative (PPD) by blastogenesis, migration inhibitory factor release (MIP), and lymphotoxin (LT) production, both before and after treatment with HTLA and complement. The HTLA was in itself highly stimulatory to PBL. However, with the addition of complement and subsequent cell destruction, a marked decrease in its stimulatory response was noted. PBL treated with HTLA and complement exhibited marked inhibition of responsiveness to con-A with little decrease in PHA-P -OR PWM stimulation except at very high concentration of HTLA. MLC reaction was inhibited only when responder cells were treated with HTLA + C'. Treatment of stimulator cells with HTLA + C' did not significantly alter the MLC response. The HTLA + C'-treated cells failed to form killer cells in the CML reaction and inhibited PPD-induced blasto-genesis from PPD-sensitized individuals; however, treatment of sensitized cells with HTLA + C' had little effects on the release of MIF and LT. It is suggested that subpopulations of T-cells carry surface antigens that bind with this specific antisera, and that the con-A-responsive cells, the responder cells in the MLC, and killer T-cells comprise a separate subset from cells responding to PHA-P or PWM, OR THE MIF-and LT-producing cells.", "contents": "Human T-cell heterogeneity as delineated with a specific human thymus lymphocyte antiserum. In vitro effects on mitogen response mixed leukocyte culture, cell-mediated lymphocytotoxicity, and lymphokine production. Human peripheral blood lymphocytes (PBL) were evaluated by their responses to phytohemmagglutinin (PHA-P), concanavallin A (con-A), and pokeweed mitogen (PWM), both before and after treatment with an antiserum against human thymic lymphocyte antigens (HTLA) that had been made T-cell-specific by multiple absorptions with immunoglobulin EAC-positive lymphoblast cell lines (B cells). Cells treated with HTLA were examined for their ability to react in a mixed lymphocyte culture (MLC) and to form killer cells in a cell-mediated lymphocytotoxicity (CML) system. Sensitized cells were also examined for their ability to respond to purified protein derivative (PPD) by blastogenesis, migration inhibitory factor release (MIP), and lymphotoxin (LT) production, both before and after treatment with HTLA and complement. The HTLA was in itself highly stimulatory to PBL. However, with the addition of complement and subsequent cell destruction, a marked decrease in its stimulatory response was noted. PBL treated with HTLA and complement exhibited marked inhibition of responsiveness to con-A with little decrease in PHA-P -OR PWM stimulation except at very high concentration of HTLA. MLC reaction was inhibited only when responder cells were treated with HTLA + C'. Treatment of stimulator cells with HTLA + C' did not significantly alter the MLC response. The HTLA + C'-treated cells failed to form killer cells in the CML reaction and inhibited PPD-induced blasto-genesis from PPD-sensitized individuals; however, treatment of sensitized cells with HTLA + C' had little effects on the release of MIF and LT. It is suggested that subpopulations of T-cells carry surface antigens that bind with this specific antisera, and that the con-A-responsive cells, the responder cells in the MLC, and killer T-cells comprise a separate subset from cells responding to PHA-P or PWM, OR THE MIF-and LT-producing cells."}
{"id": "PMID:1091658", "title": "Peripheral blood T and B lymphocytes during acute rheumatic fever.", "content": "Proportions and total numbers of thymus-derived (T) and bone marrow-derived (B) peripheral blood lymphocytes were studied in 53 patients with acute rheumatic fever, diagnosed on the basis of modifified Jones criteria. An elevation in both proportions and absolute numbers of cells bearing surface Ig was found in most patients, particularly during the first 7 days after onset. Conversely, T-cell proportions and numbers were often found to be depressed early in the acue phases of rheumatic fever. Proportions of cells bearing surface Ig did not correlate with another B-cell marker, the aggregated gamma globulin receptor, suggesting that such cells bearing surface Ig were not all B lymphocytes. Incuvation for 20 h at 37 per cent C of cells showing high proportions of surface Ig-bearing surface Ig in both normal and rheumatic fever subjects, although there was no appreciable increment in proportions of lymphocytes expressing T-cell markers. Patients with initial attacks showed higher percentages and total numbers of Ig-bearing lymphocytes (P smaller than 0.01) than did those with rneumatic fever recurrences. Elevations in numbers and proportions of peripheral blood lymphocytes bearing Ig appeared to correlate with the relative acute nature of the rheumatic fever attack.", "contents": "Peripheral blood T and B lymphocytes during acute rheumatic fever. Proportions and total numbers of thymus-derived (T) and bone marrow-derived (B) peripheral blood lymphocytes were studied in 53 patients with acute rheumatic fever, diagnosed on the basis of modifified Jones criteria. An elevation in both proportions and absolute numbers of cells bearing surface Ig was found in most patients, particularly during the first 7 days after onset. Conversely, T-cell proportions and numbers were often found to be depressed early in the acue phases of rheumatic fever. Proportions of cells bearing surface Ig did not correlate with another B-cell marker, the aggregated gamma globulin receptor, suggesting that such cells bearing surface Ig were not all B lymphocytes. Incuvation for 20 h at 37 per cent C of cells showing high proportions of surface Ig-bearing surface Ig in both normal and rheumatic fever subjects, although there was no appreciable increment in proportions of lymphocytes expressing T-cell markers. Patients with initial attacks showed higher percentages and total numbers of Ig-bearing lymphocytes (P smaller than 0.01) than did those with rneumatic fever recurrences. Elevations in numbers and proportions of peripheral blood lymphocytes bearing Ig appeared to correlate with the relative acute nature of the rheumatic fever attack."}
{"id": "PMID:1091659", "title": "Excess intravascular coagulation complicating low cardiac output.", "content": "In 42 children with congenital heart disease coagulation factor levels were studied serially during the first 20 hours following cardiopulmonary bypass surgery. The acyanotic patients, and also cyanotic patients who survived the operation, showed a progressive improvement in their coagulation profile from initial low postoperative levels. In 12 cyanotic patients who died within 72 hours, however, the coagulation factor levels either remained low, or fell further, until death. Fresh frozen plasma was administered to eight of these patients without apparent benefit. The abnormal coagulation profile correlated significantly with low skin temperature and increased blood loss and was considered to represent excess intravascular coagulation secondary to low cardiac output and poor tissue perfusion.", "contents": "Excess intravascular coagulation complicating low cardiac output. In 42 children with congenital heart disease coagulation factor levels were studied serially during the first 20 hours following cardiopulmonary bypass surgery. The acyanotic patients, and also cyanotic patients who survived the operation, showed a progressive improvement in their coagulation profile from initial low postoperative levels. In 12 cyanotic patients who died within 72 hours, however, the coagulation factor levels either remained low, or fell further, until death. Fresh frozen plasma was administered to eight of these patients without apparent benefit. The abnormal coagulation profile correlated significantly with low skin temperature and increased blood loss and was considered to represent excess intravascular coagulation secondary to low cardiac output and poor tissue perfusion."}
{"id": "PMID:1091660", "title": "The morphological identification of pathogenic yeasts using carbohydrate media.", "content": "Eight isolates of C. albicans were used to determine the frequency with which germ tube formation occurred: on rice extract -Tween 80 agar, on its components, and on 1% bactopeptone agar after three hr at 37 degrees C; in 0.5% aqueous solution of various carbohydrates; in various concentrations of glucose; on 0.5 and 0.1% glucose agar and on various types of agar alone. Subsequently 250 isolates of yeast of the genera Candida, Torulopsis, Trichosporon, Cryptococcus, and Saccharomyces, which were obtained from a clinical laboratory, were spread on rice extract -Tween 80 agar and on 0.1% glucose agar and covered with coverslips. Direct microscopic examination after incubation for three hours at 37 degrees C demonstrated germ tube formation by all 140 isolates of C. albicans, but by none of the other yeasts. The characteristic features of the pseudomycelia of isolates of Candida and Trichosporon were evident on reexamination after a further 45 to 69 hours at room temperature (22 degrees C). These morphological observations suggested the identity of the isolates of Torulopsis, Cryptococcus, and Saccharomyces but identified virtually all (98.2%) of those of the genera which formed pseudomycelia. Of the latter group only four isolates required fermentation and assimilation tests to determine whether they were C. parapsilosis (1) or C. guilliermondii (3).", "contents": "The morphological identification of pathogenic yeasts using carbohydrate media. Eight isolates of C. albicans were used to determine the frequency with which germ tube formation occurred: on rice extract -Tween 80 agar, on its components, and on 1% bactopeptone agar after three hr at 37 degrees C; in 0.5% aqueous solution of various carbohydrates; in various concentrations of glucose; on 0.5 and 0.1% glucose agar and on various types of agar alone. Subsequently 250 isolates of yeast of the genera Candida, Torulopsis, Trichosporon, Cryptococcus, and Saccharomyces, which were obtained from a clinical laboratory, were spread on rice extract -Tween 80 agar and on 0.1% glucose agar and covered with coverslips. Direct microscopic examination after incubation for three hours at 37 degrees C demonstrated germ tube formation by all 140 isolates of C. albicans, but by none of the other yeasts. The characteristic features of the pseudomycelia of isolates of Candida and Trichosporon were evident on reexamination after a further 45 to 69 hours at room temperature (22 degrees C). These morphological observations suggested the identity of the isolates of Torulopsis, Cryptococcus, and Saccharomyces but identified virtually all (98.2%) of those of the genera which formed pseudomycelia. Of the latter group only four isolates required fermentation and assimilation tests to determine whether they were C. parapsilosis (1) or C. guilliermondii (3)."}
{"id": "PMID:1091661", "title": "Comparison of different media for bacteriocine typing of Proteus mirabilis.", "content": "The effect of a variety of nutrient bases on the production of bacteriocines by 10 standard Cradock-Watson types of Proteus mirabilis has been investigated. Differences in yield of greater than 10,000 fold were observed. Maximum yields were obtained with proteose peptone no. 3 (Difco) and a medium containing this with oxgall (Difco) as an antiswarming agent was developed for routine typing.", "contents": "Comparison of different media for bacteriocine typing of Proteus mirabilis. The effect of a variety of nutrient bases on the production of bacteriocines by 10 standard Cradock-Watson types of Proteus mirabilis has been investigated. Differences in yield of greater than 10,000 fold were observed. Maximum yields were obtained with proteose peptone no. 3 (Difco) and a medium containing this with oxgall (Difco) as an antiswarming agent was developed for routine typing."}
{"id": "PMID:1091662", "title": "Titres and cross reactions of commercial antisera for the capsular typing of Klebsiella species.", "content": "Ninety commercially supplied sera for the capsular typing of Klebsiella species were tested in a full chequerboard against 72 type strains. Only four of 144 homologous reactions could not be detected. Of the 6336 possible heterologous reactions, 91 actual cross reactions were found. All positive reactions were titrated and working dilutions deduced. This provides essential information for the successful typing of unknown strains with these sera.", "contents": "Titres and cross reactions of commercial antisera for the capsular typing of Klebsiella species. Ninety commercially supplied sera for the capsular typing of Klebsiella species were tested in a full chequerboard against 72 type strains. Only four of 144 homologous reactions could not be detected. Of the 6336 possible heterologous reactions, 91 actual cross reactions were found. All positive reactions were titrated and working dilutions deduced. This provides essential information for the successful typing of unknown strains with these sera."}
{"id": "PMID:1091663", "title": "The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis.", "content": "Three types of antibody curve have been demonstrated by testing sera during the course of acquired toxoplasmosis by six different techniques. These three types are due to cell-wall antibody, (demonstrated by four of the techniques), to antibody to soluble antigen, and to IgM antibody to the cell wall. These findings have been supported by absorption experiments. A scheme is presented for testing single sera by two or three different tests to indicate the stage and duration of the infection.", "contents": "The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis. Three types of antibody curve have been demonstrated by testing sera during the course of acquired toxoplasmosis by six different techniques. These three types are due to cell-wall antibody, (demonstrated by four of the techniques), to antibody to soluble antigen, and to IgM antibody to the cell wall. These findings have been supported by absorption experiments. A scheme is presented for testing single sera by two or three different tests to indicate the stage and duration of the infection."}
{"id": "PMID:1091667", "title": "Relations between auditory nerve endings and cell types in the cat's anteroventral cochlear nucleus seen with the Golgi method and Nomarski optics.", "content": "Rapid Golgi impregnations of the ascending branches of the auditory nerve fibers and of the types of neurons in the anteroventral cochlear nucleus (AVCN) were studied. Entire ascending branches could be observed, some of these branches project to each subdivision, others do not. There are two main typesof large neurons: the bushy and stellate cells. Criteria were established for identifying unimpregnated bushy and stellate perikarya by means of Nomarski optics, and these criteria were checked by Momarski observations on neurons which had either impregnated dendrites and unimpregnated cell bodies or impregnated portions of perikarya. In this way, the relations of unimpregnated cell bodies to auditory nerve endings were observed. Furthermore, with Nomarski optics, the cytoarchitectonic subdivisions of AVCN could be determined. Differences in the end-bulbs and collateral endings formed by the auditory nerve fibers were distinguished in three of the cytoarchitectonic subdivisions of the AVCN. End-bulbs in the anterior division were much larger than those in the dorsal and ventral parts of the posterior division. The large end-bulbs of Held in the anterior division of the AVCN were consistently associated with the perikarya of bushy cells and not with those of stellate cells. The large end-bulbs are not observed in the posterior division. Thus, bushy cells in the posterior division, although morphologically similar to those in the anterior division, must have a different synaptic organization. This difference may correspond to electrophysiological distinctions in the time-patterns of response recorded in these regions following acoustic stimulation.", "contents": "Relations between auditory nerve endings and cell types in the cat's anteroventral cochlear nucleus seen with the Golgi method and Nomarski optics. Rapid Golgi impregnations of the ascending branches of the auditory nerve fibers and of the types of neurons in the anteroventral cochlear nucleus (AVCN) were studied. Entire ascending branches could be observed, some of these branches project to each subdivision, others do not. There are two main typesof large neurons: the bushy and stellate cells. Criteria were established for identifying unimpregnated bushy and stellate perikarya by means of Nomarski optics, and these criteria were checked by Momarski observations on neurons which had either impregnated dendrites and unimpregnated cell bodies or impregnated portions of perikarya. In this way, the relations of unimpregnated cell bodies to auditory nerve endings were observed. Furthermore, with Nomarski optics, the cytoarchitectonic subdivisions of AVCN could be determined. Differences in the end-bulbs and collateral endings formed by the auditory nerve fibers were distinguished in three of the cytoarchitectonic subdivisions of the AVCN. End-bulbs in the anterior division were much larger than those in the dorsal and ventral parts of the posterior division. The large end-bulbs of Held in the anterior division of the AVCN were consistently associated with the perikarya of bushy cells and not with those of stellate cells. The large end-bulbs are not observed in the posterior division. Thus, bushy cells in the posterior division, although morphologically similar to those in the anterior division, must have a different synaptic organization. This difference may correspond to electrophysiological distinctions in the time-patterns of response recorded in these regions following acoustic stimulation."}
{"id": "PMID:1091668", "title": "Prefrontal control of conditioned suppression and associated cardiovascular variables in the monkey (Macaca mulatta).", "content": "Rhesus monkeys were trained in a discriminated conditioned emotional responses paradigm. The discriminated conditioned response (CR) complex consisted of bar-press suppression, increased terminal aortic blood flow, and increased arterial pressure. Bar-press suppression and blood flow responses were temporarily eliminated by single-stage bilateral prefrontal lobectomy but not by extensive posterior cortical ablation, 2-stage prefrontal lobe removal, or 3-stage prefrontal cortical surface ablation. The blood pressure CR was attenuated by almost every surgical operation. The changes in heart rate paralleled changes in general activity. These data indicate that the prefrontal lobes influence the conditioned suppression of ongoing rewarded behavior and the vascular responses accompanying the suppression. The time course for recovery of the somatic CR after lobectomy differed from those of the cardiovascular CRs.", "contents": "Prefrontal control of conditioned suppression and associated cardiovascular variables in the monkey (Macaca mulatta). Rhesus monkeys were trained in a discriminated conditioned emotional responses paradigm. The discriminated conditioned response (CR) complex consisted of bar-press suppression, increased terminal aortic blood flow, and increased arterial pressure. Bar-press suppression and blood flow responses were temporarily eliminated by single-stage bilateral prefrontal lobectomy but not by extensive posterior cortical ablation, 2-stage prefrontal lobe removal, or 3-stage prefrontal cortical surface ablation. The blood pressure CR was attenuated by almost every surgical operation. The changes in heart rate paralleled changes in general activity. These data indicate that the prefrontal lobes influence the conditioned suppression of ongoing rewarded behavior and the vascular responses accompanying the suppression. The time course for recovery of the somatic CR after lobectomy differed from those of the cardiovascular CRs."}
{"id": "PMID:1091669", "title": "Separation of the glycopeptides released by the action of rennin on whole milk.", "content": "A comparison has been made of a number of different elution conditions for the separation by ion-exchange chromatography of the glycopeptides released by the action of rennin on whole milk. It was found that the most satisfactory separation was achieved using an NaCl gradient (0.10-0.18 M) in 0.049 M-KH2PO4+0.001 M-Na2HPO4 at a constant pH of 5.1.", "contents": "Separation of the glycopeptides released by the action of rennin on whole milk. A comparison has been made of a number of different elution conditions for the separation by ion-exchange chromatography of the glycopeptides released by the action of rennin on whole milk. It was found that the most satisfactory separation was achieved using an NaCl gradient (0.10-0.18 M) in 0.049 M-KH2PO4+0.001 M-Na2HPO4 at a constant pH of 5.1."}
{"id": "PMID:1091664", "title": "Analgesic effect of delta-9-tetrahydrocannabinol.", "content": "A preliminary trial of oral delta-9-tetrahydrocannabinol (THC) demonstrated an analgesic effect of the drug in patients experiencing cancer pain. Placebo and 5, 10, 15, and 20 mg THC were administered double blind to ten patients. Pain relief significantly superior to placebo was demonstrated at high dose levels (15 and 20 mg). At these levels, substantial sedation and mental clouding were reported.", "contents": "Analgesic effect of delta-9-tetrahydrocannabinol. A preliminary trial of oral delta-9-tetrahydrocannabinol (THC) demonstrated an analgesic effect of the drug in patients experiencing cancer pain. Placebo and 5, 10, 15, and 20 mg THC were administered double blind to ten patients. Pain relief significantly superior to placebo was demonstrated at high dose levels (15 and 20 mg). At these levels, substantial sedation and mental clouding were reported."}
{"id": "PMID:1091665", "title": "Plasma levels and clinical effects of thioridazine and thiothixene.", "content": "The effects of thioridazine and thiothixene were studied by a double-blind technique on 40 schizophrenic patients. The doses were adjusted for optimal clinical and therapeutic effects and side effects were rated after three and eight weeks of treatment. No statistically significant differences were observed between the two drugs or between either of the two drugs and the previous medication. Plasma levels were estimated by a fluorometric technique after three and eight weeks of treatment. No correlation was found between plasma levels and clinical effects for either thioridazine or thiothixene. Plasma levels of both drugs were clearly correlated to dosage after three weeks of treatment. After eight weeks this correlation persisted for thioridazine but not for thiothixene. By that time plasma levels of thiothixene had decreased to about 30 per cent of the initial value, indicating strong enzyme induction.", "contents": "Plasma levels and clinical effects of thioridazine and thiothixene. The effects of thioridazine and thiothixene were studied by a double-blind technique on 40 schizophrenic patients. The doses were adjusted for optimal clinical and therapeutic effects and side effects were rated after three and eight weeks of treatment. No statistically significant differences were observed between the two drugs or between either of the two drugs and the previous medication. Plasma levels were estimated by a fluorometric technique after three and eight weeks of treatment. No correlation was found between plasma levels and clinical effects for either thioridazine or thiothixene. Plasma levels of both drugs were clearly correlated to dosage after three weeks of treatment. After eight weeks this correlation persisted for thioridazine but not for thiothixene. By that time plasma levels of thiothixene had decreased to about 30 per cent of the initial value, indicating strong enzyme induction."}
{"id": "PMID:1091685", "title": "Histologic studies of human skin test responses to ragweed and compound 48/80. II. Effects of corticosteroid therapy.", "content": "In a controlled study, a 1-week course of a moderate dosage of corticosteroids given to ragweed-sensitive subjects was associated with a statistically significant decrease in blood eosinophi, levels and tissue esosinophil, but not mast cell, responses to ragweed antibody levels or gross whealing responses to antigen or compound 48/80. While these findings suggest indirectly that a major component of the corticosteroid effect is likely an alteration of the mobilization of eosinophils to the inflammatory site, additional studies will be required to more directly characterize the mechanisms involved.", "contents": "Histologic studies of human skin test responses to ragweed and compound 48/80. II. Effects of corticosteroid therapy. In a controlled study, a 1-week course of a moderate dosage of corticosteroids given to ragweed-sensitive subjects was associated with a statistically significant decrease in blood eosinophi, levels and tissue esosinophil, but not mast cell, responses to ragweed antibody levels or gross whealing responses to antigen or compound 48/80. While these findings suggest indirectly that a major component of the corticosteroid effect is likely an alteration of the mobilization of eosinophils to the inflammatory site, additional studies will be required to more directly characterize the mechanisms involved."}
{"id": "PMID:1091686", "title": "Effects of diphenylhydantoin on mental abilities in the elderly.", "content": "An assessment was made of the effects of diphenylhydantoin (DPH) on the mental functions of clinically normal elderly persons. Their basic intelligence levels ranged between normal and bright-normal and they represented middle and lower-upper socio-economic group. The 10 subjects, 4 male and 6 female volunteers (average age, 69.3 years), were given pretrial intelligence tests, repeated twice during a double-blind, cross-over study of drug and placebo effects. Each cross-over period lasted three weeks. The dosage of DPH (Dilantin) was 100 mg twice daily, and placebo capsuales of identical appearance were given on the same schedule. The Wechsler Intelligence Scale series in three alternate forms was used to measure specific mental functions. The weighted scores of the altervate forms were directly compared to measure the difference between drug and placebo conditions. Significant increases in favor of DPH were observed in the Information, Comprehension, and Digit Symbol subtests, and in the Verbal Scale and Full Scale Intelligence Quotients. The first two measures indicated significant improvement in long-term memory and social comprehension. The third reflected an increase in the ability to learn new material and increased speed in visual-motor coordination. The fourth and fifth showed the effectiveness of DPH in improving general mental functioning. Apparently the underlying factor synthesizing these general DPH-associated improvements is mental concentration.", "contents": "Effects of diphenylhydantoin on mental abilities in the elderly. An assessment was made of the effects of diphenylhydantoin (DPH) on the mental functions of clinically normal elderly persons. Their basic intelligence levels ranged between normal and bright-normal and they represented middle and lower-upper socio-economic group. The 10 subjects, 4 male and 6 female volunteers (average age, 69.3 years), were given pretrial intelligence tests, repeated twice during a double-blind, cross-over study of drug and placebo effects. Each cross-over period lasted three weeks. The dosage of DPH (Dilantin) was 100 mg twice daily, and placebo capsuales of identical appearance were given on the same schedule. The Wechsler Intelligence Scale series in three alternate forms was used to measure specific mental functions. The weighted scores of the altervate forms were directly compared to measure the difference between drug and placebo conditions. Significant increases in favor of DPH were observed in the Information, Comprehension, and Digit Symbol subtests, and in the Verbal Scale and Full Scale Intelligence Quotients. The first two measures indicated significant improvement in long-term memory and social comprehension. The third reflected an increase in the ability to learn new material and increased speed in visual-motor coordination. The fourth and fifth showed the effectiveness of DPH in improving general mental functioning. Apparently the underlying factor synthesizing these general DPH-associated improvements is mental concentration."}
{"id": "PMID:1091692", "title": "Specificity of brain-reactive antibodies in serum of old mice.", "content": "Previous studies in our laboratory demonstrated that brain-reactive antibodies are present in the serum of old mice and these are separated from the brain antigen by the blood-brain barrier. In this paper the specificity of these antibodies to neuronal antigen has been studied using sera from old mice with or without absorption by the homogenates of different organs and also by direct treatment with sections of those organs. The results indicate that these brain-reactive antibodies found in sera of old mice are specific to neuronal antigen in the central nervous system.", "contents": "Specificity of brain-reactive antibodies in serum of old mice. Previous studies in our laboratory demonstrated that brain-reactive antibodies are present in the serum of old mice and these are separated from the brain antigen by the blood-brain barrier. In this paper the specificity of these antibodies to neuronal antigen has been studied using sera from old mice with or without absorption by the homogenates of different organs and also by direct treatment with sections of those organs. The results indicate that these brain-reactive antibodies found in sera of old mice are specific to neuronal antigen in the central nervous system."}
{"id": "PMID:1091693", "title": "Normal function of transplanted marrow cell lines from aged mice.", "content": "Transplantation experiments indicated that losses with age in erythrocyte production were not intrinsically timed within marrow cell lines. In most cases marrow cell lines from old donors functioned as well as those from young donors after transplantation into either W/W-v anemic or lethally irradiated normal recipients. After normal marrow cells had been serially transplanted into successive W/W-v mice 5 times, both old and young cell lines began to fail; the old cell lines had produced erythrocytes normally for 77 to 84 mo. Transplanted old and young control marrow cell lines, identified by T6 chromosomes, saved the lives of lethally irradiated recipients; the oldest cell lines functioned normally for 54 mo. The hypothesis is suggested that senescence of an organism is caused by intrinsically timed functional declines in only a few vital cell types. In transplantation experiments to identify these cell types, four criteria--function, identification, control, and health--should be met. The marrow transplantation experiments led to and illustrate the hypothesis and the four criteria.", "contents": "Normal function of transplanted marrow cell lines from aged mice. Transplantation experiments indicated that losses with age in erythrocyte production were not intrinsically timed within marrow cell lines. In most cases marrow cell lines from old donors functioned as well as those from young donors after transplantation into either W/W-v anemic or lethally irradiated normal recipients. After normal marrow cells had been serially transplanted into successive W/W-v mice 5 times, both old and young cell lines began to fail; the old cell lines had produced erythrocytes normally for 77 to 84 mo. Transplanted old and young control marrow cell lines, identified by T6 chromosomes, saved the lives of lethally irradiated recipients; the oldest cell lines functioned normally for 54 mo. The hypothesis is suggested that senescence of an organism is caused by intrinsically timed functional declines in only a few vital cell types. In transplantation experiments to identify these cell types, four criteria--function, identification, control, and health--should be met. The marrow transplantation experiments led to and illustrate the hypothesis and the four criteria."}
{"id": "PMID:1091694", "title": "Defective erythropoietic responses of aged mice not improved by young marrow.", "content": "Defective responses to severe bleeding in 25-mo.-old C57BL/6J mice were not improved by injections of 8-mo.-old marrow cells. Such injections greatly improved responses of genetically anemic W/W-v mice that have defective erythropoietic stem cells. Apparently the defective responses of old mice were not intrinsic to their erythropoietic stem cells.", "contents": "Defective erythropoietic responses of aged mice not improved by young marrow. Defective responses to severe bleeding in 25-mo.-old C57BL/6J mice were not improved by injections of 8-mo.-old marrow cells. Such injections greatly improved responses of genetically anemic W/W-v mice that have defective erythropoietic stem cells. Apparently the defective responses of old mice were not intrinsic to their erythropoietic stem cells."}
{"id": "PMID:1091696", "title": "Diarrhoea in general practice: a sixteen-year report of investigations in a microbiology laboratory, with epidemiological assessment.", "content": "Results are presented of the laboratory examination of faeces specimens from  20,273 patients with acute diarrhoea. These were household index cases seen in general practice in a London borough during the years 1953-68. An annual average of about 2 per cent of households in the area were affected, but there was considerable fluctuation with year and season. Half the patients were children although only one-fifth of the population at risk was under 15 years of age. The greatest incidence of diarrhoea was among children under 5 years old. Male children, but female adults predominated. Specimens were sent for laboratory diagnosis at the discretion of the general practitioner. The laboratory found some abnormality in nearly a third and there were indications that transmissible infection was involved in about one-fifth of patients. The most common diagnosis was Sonne dysentery (9 per cent) which came in epidemic waves and made its greatest impact among young school children. Microscopy was useful, and giardiasis was diagnosed in 1-4 per cent of index patients. Other parasites were less commonly found. Fatty globules characteristic of an infectious condition we have called 'fatty diarrhoea' were frequently observed by microscopy in stools from young children and occasionally from older persons. Blood or pus cells were seen in less than half the shigella and salmonella infections and in a much smaller proportion of the remainder. A test for occult blood performed on specimens from all patients of 40 years or older was positive, in the absence of visible red cells, in a tenth of these cases. Other studies on the bacteriology of diarrhoea in general practice are referred to and some epidemiological comparisons made. The possible place of unidentified infective agents in the aetiology of undiagnosed diarrhoeas and of 'fatty diarrhoea' is discussed.", "contents": "Diarrhoea in general practice: a sixteen-year report of investigations in a microbiology laboratory, with epidemiological assessment. Results are presented of the laboratory examination of faeces specimens from  20,273 patients with acute diarrhoea. These were household index cases seen in general practice in a London borough during the years 1953-68. An annual average of about 2 per cent of households in the area were affected, but there was considerable fluctuation with year and season. Half the patients were children although only one-fifth of the population at risk was under 15 years of age. The greatest incidence of diarrhoea was among children under 5 years old. Male children, but female adults predominated. Specimens were sent for laboratory diagnosis at the discretion of the general practitioner. The laboratory found some abnormality in nearly a third and there were indications that transmissible infection was involved in about one-fifth of patients. The most common diagnosis was Sonne dysentery (9 per cent) which came in epidemic waves and made its greatest impact among young school children. Microscopy was useful, and giardiasis was diagnosed in 1-4 per cent of index patients. Other parasites were less commonly found. Fatty globules characteristic of an infectious condition we have called 'fatty diarrhoea' were frequently observed by microscopy in stools from young children and occasionally from older persons. Blood or pus cells were seen in less than half the shigella and salmonella infections and in a much smaller proportion of the remainder. A test for occult blood performed on specimens from all patients of 40 years or older was positive, in the absence of visible red cells, in a tenth of these cases. Other studies on the bacteriology of diarrhoea in general practice are referred to and some epidemiological comparisons made. The possible place of unidentified infective agents in the aetiology of undiagnosed diarrhoeas and of 'fatty diarrhoea' is discussed."}
{"id": "PMID:1091697", "title": "Incubation at raised temperature or enrichment media, combined with secondary enrichment in Rappaport's medium, for the isolation of salmonellas from sewage.", "content": "Two FMDV strains which had been previously differentiated by complement-fixation were compared by guinea-pig protection test, kinetic neutralization and micro-neutralization tests. It was found that these tests, which have not been previously applied by the methods described, were all capable of FMDV STRAIN DIFFERENTIATION. Similar differences were found by all methods, which suggests that comparisons made by cross-CF, cross-neutr-alization or cross-protection involve measurement of the same antigen/antibody interactions.", "contents": "Incubation at raised temperature or enrichment media, combined with secondary enrichment in Rappaport's medium, for the isolation of salmonellas from sewage. Two FMDV strains which had been previously differentiated by complement-fixation were compared by guinea-pig protection test, kinetic neutralization and micro-neutralization tests. It was found that these tests, which have not been previously applied by the methods described, were all capable of FMDV STRAIN DIFFERENTIATION. Similar differences were found by all methods, which suggests that comparisons made by cross-CF, cross-neutr-alization or cross-protection involve measurement of the same antigen/antibody interactions."}
{"id": "PMID:1091698", "title": "The problem and implications of chloramphenicol resistance in the typhoid bacillus.", "content": "Transferable chloramphenicol resistance has become common in the typhoid bacillus in countries such as Mexico, India, Vietnam and Thailand. Situations such as this, and others analogous to it in many parts of the world, are the result of the long-term indiscriminate use of chloramphenicol and other antibiotics in the affected areas. They can be rectified only by more rational antibiotic usage.", "contents": "The problem and implications of chloramphenicol resistance in the typhoid bacillus. Transferable chloramphenicol resistance has become common in the typhoid bacillus in countries such as Mexico, India, Vietnam and Thailand. Situations such as this, and others analogous to it in many parts of the world, are the result of the long-term indiscriminate use of chloramphenicol and other antibiotics in the affected areas. They can be rectified only by more rational antibiotic usage."}
{"id": "PMID:1091699", "title": "Canine marrow transplantation: are serum blocking factors necessary to maintain the stable chimeric state?", "content": "Nine long-term canine radiation chimeras and their canine histocompatibility locus (DL-A) compatible, mixed leukocyte culture negative littermate marrow donors were studied between 545 and 1282 days after 1,200 R total body irradiation and marrow grafting. Before the time of testing, marrow donors were immunized against their chimeras by repeated skin grafts which they rejected. Skin fibroblasts from chimeras and their donors were tested for cell inhibition (CI) in the microcytotoxicity assay be exposure to lymphocytes from chimeras, donors and normal dogs and the effects of various sera on CI were evaluated. Lymphocytes from sensitized marrow donors consistently inhibited fibroblasts from the chimeras (eight of nine dogs); CI was abrogated by chimeric serum in only three of eight cases. Only two chimeras showed consistent CI of their \"own\" fibroblasts; CI was blocked by chimeric serum in one of the two. The remaining seven chimeras did not show consistent CI. Sequential studies in 16 additional recipients of DL-A compatible littermate marrow were carried out from 45 to 439 days after marrow grafting. Seven of the 16 did not show CI of chimeric fibroblasts by chimeric lymphocytes at any time. Nine showed CI on one or several occasions. Serum blocking factors were seen on one occasion in each of two chimeras. In conclusion, the CI assay is able to detect immunity against \"minor\" histocompatibility systems in dogs. Both long- and short-term chimeras occasionally demonstrated CI of chimeric fibroblasts but serum blocking factors did not appear to be necessary for maintaining stable graft-host \"tolerance.\"", "contents": "Canine marrow transplantation: are serum blocking factors necessary to maintain the stable chimeric state? Nine long-term canine radiation chimeras and their canine histocompatibility locus (DL-A) compatible, mixed leukocyte culture negative littermate marrow donors were studied between 545 and 1282 days after 1,200 R total body irradiation and marrow grafting. Before the time of testing, marrow donors were immunized against their chimeras by repeated skin grafts which they rejected. Skin fibroblasts from chimeras and their donors were tested for cell inhibition (CI) in the microcytotoxicity assay be exposure to lymphocytes from chimeras, donors and normal dogs and the effects of various sera on CI were evaluated. Lymphocytes from sensitized marrow donors consistently inhibited fibroblasts from the chimeras (eight of nine dogs); CI was abrogated by chimeric serum in only three of eight cases. Only two chimeras showed consistent CI of their \"own\" fibroblasts; CI was blocked by chimeric serum in one of the two. The remaining seven chimeras did not show consistent CI. Sequential studies in 16 additional recipients of DL-A compatible littermate marrow were carried out from 45 to 439 days after marrow grafting. Seven of the 16 did not show CI of chimeric fibroblasts by chimeric lymphocytes at any time. Nine showed CI on one or several occasions. Serum blocking factors were seen on one occasion in each of two chimeras. In conclusion, the CI assay is able to detect immunity against \"minor\" histocompatibility systems in dogs. Both long- and short-term chimeras occasionally demonstrated CI of chimeric fibroblasts but serum blocking factors did not appear to be necessary for maintaining stable graft-host \"tolerance.\""}
{"id": "PMID:1091700", "title": "Canine radiation chimeras: an attempt to demonstrate serum blocking factors by an in vivo approach.", "content": "An attempt was made to demonstrate in vivo the presence of blocking factors in the serum of canine chimeras with grafts of chimeric skin onto the marrow donor as an indicator system. Even very large amounts of chimeric serum (one-fourth of the plasma volume infused daily from day 2 before skin grafting until rejection) failed to modify first and third set skin graft rejection patterns. Thus, serum blocking factors could not be demonstrated by this in vivo approach.", "contents": "Canine radiation chimeras: an attempt to demonstrate serum blocking factors by an in vivo approach. An attempt was made to demonstrate in vivo the presence of blocking factors in the serum of canine chimeras with grafts of chimeric skin onto the marrow donor as an indicator system. Even very large amounts of chimeric serum (one-fourth of the plasma volume infused daily from day 2 before skin grafting until rejection) failed to modify first and third set skin graft rejection patterns. Thus, serum blocking factors could not be demonstrated by this in vivo approach."}
{"id": "PMID:1091701", "title": "The production of anti-guinea pig lymphokine antibody.", "content": "Rabbit antiserum to lymphokine-containing supernatant fluids has been prepared by means of a two-stage immunization procedure. Migration inhibiting factor (MIF)-containing and control supernatants were obtained from guinea pig lymphocyte cultures. Both were fractionated on Sephadex G-100. Fractions of control supernatants corresponding to active fractions from the MIF-rich material were used to immunize rabbits. The anti-\"control\" antibody so obtained was conjugated to agarose beads to prepare an immunoadsorbent column. The MIF-rich Sephadex fractions were passed through this column to remove materials corresponding to those found in control supernatants and which could react with the anti-control antibody. The eluted MIF-rich fraction was concentrated and used to immunize a second group of rabbits. The anti-\"lymphokine\" antiserum so obtained was studied for its effect in vitro and in vivo. It was found capable of suppressing the activity of MIF, macrophage chemotactic factor, and skin reactive factor. It could also suppress delayed hypersensitivity skin reactions in actively immunized guinea pigs.", "contents": "The production of anti-guinea pig lymphokine antibody. Rabbit antiserum to lymphokine-containing supernatant fluids has been prepared by means of a two-stage immunization procedure. Migration inhibiting factor (MIF)-containing and control supernatants were obtained from guinea pig lymphocyte cultures. Both were fractionated on Sephadex G-100. Fractions of control supernatants corresponding to active fractions from the MIF-rich material were used to immunize rabbits. The anti-\"control\" antibody so obtained was conjugated to agarose beads to prepare an immunoadsorbent column. The MIF-rich Sephadex fractions were passed through this column to remove materials corresponding to those found in control supernatants and which could react with the anti-control antibody. The eluted MIF-rich fraction was concentrated and used to immunize a second group of rabbits. The anti-\"lymphokine\" antiserum so obtained was studied for its effect in vitro and in vivo. It was found capable of suppressing the activity of MIF, macrophage chemotactic factor, and skin reactive factor. It could also suppress delayed hypersensitivity skin reactions in actively immunized guinea pigs."}
{"id": "PMID:1091702", "title": "Differential regulatory effects of accessory cells on the generation of cell-mediated immune reactions.", "content": "Conditions for the in vitro generation of cell-mediated immunity to H-2 and non-H-2 alloantigens by mouse spleen cells were compared to conditions for generation of in vitro humoral immunity to sheep red cells. Normal spleen cells grown in standard or allogeneic cell-conditioned media developed strong cellular responses to H-2 antigens but little or no response to non-H-2 antigens. Subpopulations of spleen cells from which the adherent cells had been removed generated strong cellular responses to both H-2 and non-H-2 antigens, provided the cells were cultured in conditioned media. Both cellular responses were shown to be mediated by T cells. Accessory cells from the peritoneum and/or spleen differentially inhibited the non H-2 cellular responses at doses which did not inhibit humoral responses.", "contents": "Differential regulatory effects of accessory cells on the generation of cell-mediated immune reactions. Conditions for the in vitro generation of cell-mediated immunity to H-2 and non-H-2 alloantigens by mouse spleen cells were compared to conditions for generation of in vitro humoral immunity to sheep red cells. Normal spleen cells grown in standard or allogeneic cell-conditioned media developed strong cellular responses to H-2 antigens but little or no response to non-H-2 antigens. Subpopulations of spleen cells from which the adherent cells had been removed generated strong cellular responses to both H-2 and non-H-2 antigens, provided the cells were cultured in conditioned media. Both cellular responses were shown to be mediated by T cells. Accessory cells from the peritoneum and/or spleen differentially inhibited the non H-2 cellular responses at doses which did not inhibit humoral responses."}
{"id": "PMID:1091703", "title": "Immune mechanisms in leukemia: evaluation of immunocompetent cell populations.", "content": "Dose-response curves of the cellular immune response of C58/wm mice to syngeneic line Ib malignant lymphoid cells (Ib cells) were computer analyzed by the PROBT subroutine in the IBM Scientific Subroutine Package. An analysis of the relative immunogenicity of various admixtures of x-irradiated (XIb) and viable Ib cells (VIb) after i.p. injection showed that the ratio had to be approximately 100:1 to be immunogenic. Viable Ib cells contained in immunogenic mixtures multiplied in vivo at a logarithmic rate up to 5 or 6 days but were eliminated immunologically by 8 or 9 days. Adoptive cell transfer techniques were used to quantify the protective effect of immune spleen cells (ISC). Essentially a constant dose of ISC (10-6.4) protected mice against a challenge dose of 10-2 to 10-5 VIb cells; more than 10-5 VIb cells were lethal. Two techniques were used to quantify immunity even though mice ultimately died of transplanted leukemia, viz., mean survival time (MST) with a fixed challenge dose of VI b cells, or MST with a fixed time for death. The sensitivity and statistical limitations of these assays are presented.  To amplify the sensitivity of assays for adoptive cellular immunity a technique of antigenic stimulation was used, viz., 1 day after x-irradiated mice (600 R) received an i.p. injection of normal or immune spleen, bone marrow or thymic cells they received an i.p. injection of XIb cells containing an admixture of VIb cells. The technique amplified the sensitivity of ISC transfer tests approximately 100-fold and made it possible to detect protective effects of bone marrow and thymic cell populations.", "contents": "Immune mechanisms in leukemia: evaluation of immunocompetent cell populations. Dose-response curves of the cellular immune response of C58/wm mice to syngeneic line Ib malignant lymphoid cells (Ib cells) were computer analyzed by the PROBT subroutine in the IBM Scientific Subroutine Package. An analysis of the relative immunogenicity of various admixtures of x-irradiated (XIb) and viable Ib cells (VIb) after i.p. injection showed that the ratio had to be approximately 100:1 to be immunogenic. Viable Ib cells contained in immunogenic mixtures multiplied in vivo at a logarithmic rate up to 5 or 6 days but were eliminated immunologically by 8 or 9 days. Adoptive cell transfer techniques were used to quantify the protective effect of immune spleen cells (ISC). Essentially a constant dose of ISC (10-6.4) protected mice against a challenge dose of 10-2 to 10-5 VIb cells; more than 10-5 VIb cells were lethal. Two techniques were used to quantify immunity even though mice ultimately died of transplanted leukemia, viz., mean survival time (MST) with a fixed challenge dose of VI b cells, or MST with a fixed time for death. The sensitivity and statistical limitations of these assays are presented.  To amplify the sensitivity of assays for adoptive cellular immunity a technique of antigenic stimulation was used, viz., 1 day after x-irradiated mice (600 R) received an i.p. injection of normal or immune spleen, bone marrow or thymic cells they received an i.p. injection of XIb cells containing an admixture of VIb cells. The technique amplified the sensitivity of ISC transfer tests approximately 100-fold and made it possible to detect protective effects of bone marrow and thymic cell populations."}
{"id": "PMID:1091704", "title": "Chemotaxis of basophils by lymphocyte-dependent and lymphocyte-independent mechanisms.", "content": "Guine pigs basophils obtained from blood or bone marrow have been studied for their chemotactic responsiveness. Chemotactic factors for basophils include a substance (lymphokine) present in culture fluids from antigen-stimulated lymphocytes, a material generated in zymosan-activated guinea pig serum, a C5 cleavage factor, and a bacterial factor. When compared with homologous neutrophils and monocytes, basophils respond most rapidly to a chemotactic stimulus. The lymphokine basophil chemotactic factor is physicochemically similar to the previously described monocyte chemotactic factor but appears to be distinct from it as well as MIF and neutrophil chemotactic factor present in the same fluids, Part of the evidence for this is the ability to detect basophil chemotactic factor in the absence of other lymphokine activities under appropriate experimental conditions. More evidence, specifically relating to the monocyte factor, is that monocytes can adsorb basophil chemotactic activity but not vice versa. This latter observation may have implications for the mechanism whereby the accumulation of basophils is controlled and limited in vivo. In addition, it was noted that specific antigen could also suppress basophil chemotaxis. Although the mechanism of this phenomenon is unclear, it could serve as a second means by which basophil accumulation may be controlled in the intact animal. Taken together, these observations provide further definition of the chemotactic behavior of basophils in general, and underscore some of the ways in which lymphocytes can influence basophils through lymphokine-dependent mechanisms.", "contents": "Chemotaxis of basophils by lymphocyte-dependent and lymphocyte-independent mechanisms. Guine pigs basophils obtained from blood or bone marrow have been studied for their chemotactic responsiveness. Chemotactic factors for basophils include a substance (lymphokine) present in culture fluids from antigen-stimulated lymphocytes, a material generated in zymosan-activated guinea pig serum, a C5 cleavage factor, and a bacterial factor. When compared with homologous neutrophils and monocytes, basophils respond most rapidly to a chemotactic stimulus. The lymphokine basophil chemotactic factor is physicochemically similar to the previously described monocyte chemotactic factor but appears to be distinct from it as well as MIF and neutrophil chemotactic factor present in the same fluids, Part of the evidence for this is the ability to detect basophil chemotactic factor in the absence of other lymphokine activities under appropriate experimental conditions. More evidence, specifically relating to the monocyte factor, is that monocytes can adsorb basophil chemotactic activity but not vice versa. This latter observation may have implications for the mechanism whereby the accumulation of basophils is controlled and limited in vivo. In addition, it was noted that specific antigen could also suppress basophil chemotaxis. Although the mechanism of this phenomenon is unclear, it could serve as a second means by which basophil accumulation may be controlled in the intact animal. Taken together, these observations provide further definition of the chemotactic behavior of basophils in general, and underscore some of the ways in which lymphocytes can influence basophils through lymphokine-dependent mechanisms."}
{"id": "PMID:1091705", "title": "Effects of an acidic polysaccharide procuced by Serratia piscatorum on immune responses in mice. I. mitogenicity and stimulation of plaque-forming cells (PFC) in vitro.", "content": "An acidic polysaccharide of Serratia piscatorum, consisting of L-rhamnose, D-galactose and D-galacturonic acid, stimulated the primary IgM PFC response of spleen cell cultures against sheep erythrocytes. The polysaccharide (PS) caused dose-dependent stimulation of 3H-thymidine incorporation into DNA of spleen cell cultures and the maximum response was seen after about 48 hr. Nonadherent B (ATXBM) cells were strongly reactive to PS, whereas adherent spleen cells were less reactive. Higher doses of PS suppressed the peak PFC response, but enhanced the earlier PFC responses. Lower doses of PS stimulated the PFC responses significantly throughout the period of PFC production. The optimal dose (0.1 mug/ml) of PS increased the PFC responses in mixed cell cultures containing spleen cells and either B cells, T cells activated with a different antigen, or hydrocortisone-resistant thymus cells of syngeneic origin. However, this dose of PS interfered with the enhanced PFC response of spleen cells due to addition of antigen-primed T cells. Since these characteristic activities of PS were like those of lipopolysaccharides (LPS), this acidic polysaccharide may be useful for further elucidating the modes of LPS action.", "contents": "Effects of an acidic polysaccharide procuced by Serratia piscatorum on immune responses in mice. I. mitogenicity and stimulation of plaque-forming cells (PFC) in vitro. An acidic polysaccharide of Serratia piscatorum, consisting of L-rhamnose, D-galactose and D-galacturonic acid, stimulated the primary IgM PFC response of spleen cell cultures against sheep erythrocytes. The polysaccharide (PS) caused dose-dependent stimulation of 3H-thymidine incorporation into DNA of spleen cell cultures and the maximum response was seen after about 48 hr. Nonadherent B (ATXBM) cells were strongly reactive to PS, whereas adherent spleen cells were less reactive. Higher doses of PS suppressed the peak PFC response, but enhanced the earlier PFC responses. Lower doses of PS stimulated the PFC responses significantly throughout the period of PFC production. The optimal dose (0.1 mug/ml) of PS increased the PFC responses in mixed cell cultures containing spleen cells and either B cells, T cells activated with a different antigen, or hydrocortisone-resistant thymus cells of syngeneic origin. However, this dose of PS interfered with the enhanced PFC response of spleen cells due to addition of antigen-primed T cells. Since these characteristic activities of PS were like those of lipopolysaccharides (LPS), this acidic polysaccharide may be useful for further elucidating the modes of LPS action."}
{"id": "PMID:1091706", "title": "DNP-Lys-ficoll: a T-independent antigen which elicits both IgM and IgG anti-DNP antibody-secreting cells.", "content": "The 2,4-dinitrophenyl-lysyl derivative of Ficoll (DNP-Lys-Ficoll) was prepared and examined for immunogenicity. This antigen elicited large numbers of DNP-specific plaque-forming cells (PFC) of the IgM and IgG2 class in the spleens of C57BL/6 mice. Similar responses were observed in congenitally athymic (nu/nu) mice and in their littermates indicating that DNP-Lys-Ficoll is a T-independent antigen. The responses of nu/nu mice included a large number of IgG2 DNP-specific PFC, indicating that IgG responses can be initiated in the absence of mature thymus-dependent (T) lymphocytes. Cell transfer studies confirmed the T independence of the response and indicated that priming with DNP-Lys-Ficoll induces only a very meager degree of memory. Because they can be obtained in large quantities and in relatively pure form, DNP-Lys-Ficoll and other hapten conjugates of Ficoll should prove most valuable in the delineation of the mode of activation of precursors of antibody-secreting cells by T-independent antigens.", "contents": "DNP-Lys-ficoll: a T-independent antigen which elicits both IgM and IgG anti-DNP antibody-secreting cells. The 2,4-dinitrophenyl-lysyl derivative of Ficoll (DNP-Lys-Ficoll) was prepared and examined for immunogenicity. This antigen elicited large numbers of DNP-specific plaque-forming cells (PFC) of the IgM and IgG2 class in the spleens of C57BL/6 mice. Similar responses were observed in congenitally athymic (nu/nu) mice and in their littermates indicating that DNP-Lys-Ficoll is a T-independent antigen. The responses of nu/nu mice included a large number of IgG2 DNP-specific PFC, indicating that IgG responses can be initiated in the absence of mature thymus-dependent (T) lymphocytes. Cell transfer studies confirmed the T independence of the response and indicated that priming with DNP-Lys-Ficoll induces only a very meager degree of memory. Because they can be obtained in large quantities and in relatively pure form, DNP-Lys-Ficoll and other hapten conjugates of Ficoll should prove most valuable in the delineation of the mode of activation of precursors of antibody-secreting cells by T-independent antigens."}
{"id": "PMID:1091707", "title": "Repopulation with IgA-containing cells of bronchial and intestinal lamina propria after transfer of homologous Peyer's patch and bronchial lymphocytes.", "content": "Transfer of 50 million rabbit allogeneic lymphocytes from either bronchus-associated lymphoid tissue (BALT) or Peyer's patches into 1000 R x-irradiated recipients results, 6 days later, in predominant repopulation of gut and bronchial lamina propria, as well as spleen with IgA-containing cells. After repopulation with BALT or Peyer's patch cells, lymphoid follicles in both gut and lung showed peripheral cellular membrane type of fluorescence with fluorescein-conjugated anti-IgA antisera only. Six days after x-irradiation alone, little evidence of repopulation was seen and immunofluorescent qualitative observations of gut and lung, and quantitative data in the spleen, confirmed these findings. After transfer of 50 million lymph node cells, very few immunoglobulin-containing cells were seen in the gut or bronchial lamina propria. These results suggest that there may be a common mucosal immunologic system, and that repopulation of gut and lung lamina propria may be through the organized lymphoid tissue therein.", "contents": "Repopulation with IgA-containing cells of bronchial and intestinal lamina propria after transfer of homologous Peyer's patch and bronchial lymphocytes. Transfer of 50 million rabbit allogeneic lymphocytes from either bronchus-associated lymphoid tissue (BALT) or Peyer's patches into 1000 R x-irradiated recipients results, 6 days later, in predominant repopulation of gut and bronchial lamina propria, as well as spleen with IgA-containing cells. After repopulation with BALT or Peyer's patch cells, lymphoid follicles in both gut and lung showed peripheral cellular membrane type of fluorescence with fluorescein-conjugated anti-IgA antisera only. Six days after x-irradiation alone, little evidence of repopulation was seen and immunofluorescent qualitative observations of gut and lung, and quantitative data in the spleen, confirmed these findings. After transfer of 50 million lymph node cells, very few immunoglobulin-containing cells were seen in the gut or bronchial lamina propria. These results suggest that there may be a common mucosal immunologic system, and that repopulation of gut and lung lamina propria may be through the organized lymphoid tissue therein."}
{"id": "PMID:1091708", "title": "Regulation of the immune response: production of a soluble suppressor by immune spleen cells in vitro.", "content": "The addition of ovalbumin-immune spleen cells (Ova ISC) to sheep erythrocytes-immune spleen cells (SRBC ISC) in Mishell-Dutton-type cultures resulted in a dramatic reduction of PFC to SRBC and was dependent upon the addition of soluble Ova at low concentrations of Ova ISC. The suppressing cells in the Ova ISC were shown to be irradiation sensitive, depleted by anti-theta antiserum and complement treatment, and did not absorb to glass bead columns. Ova ISC-induced inhibition also occurred in culture chambers across a cell-impermeable membrane and a soluble inhibitor was recovered in chambers opposite the Ova ISC. This suppressor factor was sensitive to trypsin treatment and to heating at 80 degrees C, but not to 70 degrees C, for 30 min. The molecular weight, as determined by sucrose gradient analysis, was between 55,000 and 60,000 daltons. This suppressor factor appears to be distinct from a T-cell \"helper\" factor which was found to be sensitive to heating at 70 degrees C for 30 min. We propose that this suppressor factor participates in the termination of most immunologic responses and is responsible for the antigenic competition phenomenon.", "contents": "Regulation of the immune response: production of a soluble suppressor by immune spleen cells in vitro. The addition of ovalbumin-immune spleen cells (Ova ISC) to sheep erythrocytes-immune spleen cells (SRBC ISC) in Mishell-Dutton-type cultures resulted in a dramatic reduction of PFC to SRBC and was dependent upon the addition of soluble Ova at low concentrations of Ova ISC. The suppressing cells in the Ova ISC were shown to be irradiation sensitive, depleted by anti-theta antiserum and complement treatment, and did not absorb to glass bead columns. Ova ISC-induced inhibition also occurred in culture chambers across a cell-impermeable membrane and a soluble inhibitor was recovered in chambers opposite the Ova ISC. This suppressor factor was sensitive to trypsin treatment and to heating at 80 degrees C, but not to 70 degrees C, for 30 min. The molecular weight, as determined by sucrose gradient analysis, was between 55,000 and 60,000 daltons. This suppressor factor appears to be distinct from a T-cell \"helper\" factor which was found to be sensitive to heating at 70 degrees C for 30 min. We propose that this suppressor factor participates in the termination of most immunologic responses and is responsible for the antigenic competition phenomenon."}
{"id": "PMID:1091713", "title": "Unilateral renal disease in the rat. I. Clinical, morphologic, and glomerular mesangial functional features of the experimental model produced by renal perfusion with aminonucleoside.", "content": "Unilateral renal perfusion with the aminonucleoside of puromycin (60 mg. per kilogram) was used to produce unilateral renal disease in rats characterized by marked proteinuria, hypoalbuminemia, and hypercholesterolemia. Urine albumin excretion increased from 0.21 plus or minus 0.16 mg. per 24 hours prior to perfusion to 126 plus or minus 30 mg. on the fourteenth day after perfusion. If the perfused kidney was removed after 10 days, urine albumin excretion immediately decreased to normal, whereas marked proteinuria continued after contralateral nephrectomy. Immunofluorescent studies revealed abundant protein reabsorption droplets (beta1C and albumin) in proximal tubules of aminonucleoside-perfused kidneys but not in contralateral kidneys thus indicating that the increased proteinuria was of unilateral origin. Less marked proteinuria was observed following unilateral renal perfusion with lower doses of aminonucleoside. Glomerular mesangial function was studied 10 days after renal perfusion. Renal specimens were obtained 12 hours after intravenous injection of 37.5 mg. per 100 Gm. of body weight of aggregated human IgG and evaluated by immunofluorescent microscopy. Glomerular mesangial staining for human IgG in aminonucleoside-perfused kidneys was markedly increased when compared to contralateral kidneys. In contrast, mesangial staining in kidneys perfused with saline was equal to that of contralateral kidneys and proteinuria following perfusion with saline was not increased. These studies provide further evidence that increased proteinuria following administration of aminonucleoside to rats is the result of a rapid direct on the kidney and that alterations of glomerular mesangial function are related to renal factors rather than changes in systemic milieu.", "contents": "Unilateral renal disease in the rat. I. Clinical, morphologic, and glomerular mesangial functional features of the experimental model produced by renal perfusion with aminonucleoside. Unilateral renal perfusion with the aminonucleoside of puromycin (60 mg. per kilogram) was used to produce unilateral renal disease in rats characterized by marked proteinuria, hypoalbuminemia, and hypercholesterolemia. Urine albumin excretion increased from 0.21 plus or minus 0.16 mg. per 24 hours prior to perfusion to 126 plus or minus 30 mg. on the fourteenth day after perfusion. If the perfused kidney was removed after 10 days, urine albumin excretion immediately decreased to normal, whereas marked proteinuria continued after contralateral nephrectomy. Immunofluorescent studies revealed abundant protein reabsorption droplets (beta1C and albumin) in proximal tubules of aminonucleoside-perfused kidneys but not in contralateral kidneys thus indicating that the increased proteinuria was of unilateral origin. Less marked proteinuria was observed following unilateral renal perfusion with lower doses of aminonucleoside. Glomerular mesangial function was studied 10 days after renal perfusion. Renal specimens were obtained 12 hours after intravenous injection of 37.5 mg. per 100 Gm. of body weight of aggregated human IgG and evaluated by immunofluorescent microscopy. Glomerular mesangial staining for human IgG in aminonucleoside-perfused kidneys was markedly increased when compared to contralateral kidneys. In contrast, mesangial staining in kidneys perfused with saline was equal to that of contralateral kidneys and proteinuria following perfusion with saline was not increased. These studies provide further evidence that increased proteinuria following administration of aminonucleoside to rats is the result of a rapid direct on the kidney and that alterations of glomerular mesangial function are related to renal factors rather than changes in systemic milieu."}
{"id": "PMID:1091717", "title": "Oxime derivatives of erythromycin: inhibitors of Rous sarcoma virus reverse transcriptase activity and focus formation.", "content": "Bovine adenovirus type 3 (BAV-3), strain WBR-1, quantitatively transforms hamster embryo carcass cells with a maximum efficiency of 4-7 X 10-4 p.f.u./focus-forming units (f.f.u). This frequency is signigicantly inhibited by the presence of 1-8 mM of Ca++. The transformed cells are highly tumourigenic in hamsters and possess T- and tumour-specific transplantation antigens. BAV-3 is one of the most oncogenic of the adenoviruses and an ideal model virus for the study of transformation.", "contents": "Oxime derivatives of erythromycin: inhibitors of Rous sarcoma virus reverse transcriptase activity and focus formation. Bovine adenovirus type 3 (BAV-3), strain WBR-1, quantitatively transforms hamster embryo carcass cells with a maximum efficiency of 4-7 X 10-4 p.f.u./focus-forming units (f.f.u). This frequency is signigicantly inhibited by the presence of 1-8 mM of Ca++. The transformed cells are highly tumourigenic in hamsters and possess T- and tumour-specific transplantation antigens. BAV-3 is one of the most oncogenic of the adenoviruses and an ideal model virus for the study of transformation."}
{"id": "PMID:1091718", "title": "Occurrence of polyamines in coliphages T5, phiX174 and in phage-infected bacteria.", "content": "The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and phiX174. Polyamines were identified by thin-layer chromatography and mass-spectrometry of dansyl derivatives, as well as by ion-exchange chromatography. In phiX174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity.", "contents": "Occurrence of polyamines in coliphages T5, phiX174 and in phage-infected bacteria. The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and phiX174. Polyamines were identified by thin-layer chromatography and mass-spectrometry of dansyl derivatives, as well as by ion-exchange chromatography. In phiX174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity."}
{"id": "PMID:1091723", "title": "Electrostimulation of the neuropathic bladder.", "content": "Intravesical electrostimulation offers promise in management of neurogenic bladder in children with myelocele. In the past 2 yr, we carried out about 500 treatments in 25 children aged 2 wk to 13 yr. After repeated stimulation, the starting intravesical pressure increases considerably in all cases; however, this was not always accompanied by clinical improvement. Our early results are encouraging, especially after repeated 10-day courses of electrostimulation; bladder contractions increase slowly and micturition begins to appear. Seventeen of 25 patients showed significant improvement, in two patients no improvement was observed. The remaining six have not been treated long enough to evaluate.", "contents": "Electrostimulation of the neuropathic bladder. Intravesical electrostimulation offers promise in management of neurogenic bladder in children with myelocele. In the past 2 yr, we carried out about 500 treatments in 25 children aged 2 wk to 13 yr. After repeated stimulation, the starting intravesical pressure increases considerably in all cases; however, this was not always accompanied by clinical improvement. Our early results are encouraging, especially after repeated 10-day courses of electrostimulation; bladder contractions increase slowly and micturition begins to appear. Seventeen of 25 patients showed significant improvement, in two patients no improvement was observed. The remaining six have not been treated long enough to evaluate."}
{"id": "PMID:1091726", "title": "The effects of endotoxemia and bacteremia on gastrointestinal drug absorption in mice and rats.", "content": "Endotoxin [lipopolysaccharide (LPS)] from four Gram-negative bacteria injected i.v. delayed absorption of drugs administered in solution by gastric tube to mice and rats. Salicylate and guinine absorption was delayed at LPS doses from 50 to 400 mug/kg. Salicylate absorption was delayed by LPS when drug was given by gastric tube, while LPS did not affect drug levels when salicylate was given i.p. or intraduodenally. Bethanechol prevented the LPS effect and LPS pretreatment also protected against delayed absorption. LPS- treated rats retained more drugs in their stomachs after 30 minutes and their plasma salicylate levels were lowered. Everted intestinal sacs from LPS-treated rats transferred salicylate as well as controls. Thus, LPS delays gastrointestinal drug absorption solely by retarding gastric emptying. Escherichia coli urinary tract infection did not reproduce LPS delay of drug absorption, but the effects of systemic bacteria were similar to those of endotoxemia.", "contents": "The effects of endotoxemia and bacteremia on gastrointestinal drug absorption in mice and rats. Endotoxin [lipopolysaccharide (LPS)] from four Gram-negative bacteria injected i.v. delayed absorption of drugs administered in solution by gastric tube to mice and rats. Salicylate and guinine absorption was delayed at LPS doses from 50 to 400 mug/kg. Salicylate absorption was delayed by LPS when drug was given by gastric tube, while LPS did not affect drug levels when salicylate was given i.p. or intraduodenally. Bethanechol prevented the LPS effect and LPS pretreatment also protected against delayed absorption. LPS- treated rats retained more drugs in their stomachs after 30 minutes and their plasma salicylate levels were lowered. Everted intestinal sacs from LPS-treated rats transferred salicylate as well as controls. Thus, LPS delays gastrointestinal drug absorption solely by retarding gastric emptying. Escherichia coli urinary tract infection did not reproduce LPS delay of drug absorption, but the effects of systemic bacteria were similar to those of endotoxemia."}
{"id": "PMID:1091727", "title": "A calcium ionophore stimulating the secretion of catecholamines from the cat adrenal.", "content": "1. Experiments were performed on perfused cat adrenal glands to examine the effect of a calcium ionophore A-23187 in the secretion of catecholamines. 2. Ionophore (1-10 muM) caused a dose-dependent release of catecholamines and the output was about 100-fold greater at 10 mum than at 1 mum. 3. Release of catecholamines by the ionophore was dependent on the calcium concentration of the perfusion medium. Omission of calcium blocked the response to the ionophore while excess calcium facilitated it. 4. Magnesium antagonized the secretory response to the ionophore. Excess calcium overcame the inhibitory effect of magnesium. 5. The ionophore did not modify release of catecholamines by induced splanchnic nerve stimulation. 6. The results suggest that the ionophore, like depolarization, introduces calcium into the chromaffin cell to cause release of catecholamines.", "contents": "A calcium ionophore stimulating the secretion of catecholamines from the cat adrenal. 1. Experiments were performed on perfused cat adrenal glands to examine the effect of a calcium ionophore A-23187 in the secretion of catecholamines. 2. Ionophore (1-10 muM) caused a dose-dependent release of catecholamines and the output was about 100-fold greater at 10 mum than at 1 mum. 3. Release of catecholamines by the ionophore was dependent on the calcium concentration of the perfusion medium. Omission of calcium blocked the response to the ionophore while excess calcium facilitated it. 4. Magnesium antagonized the secretory response to the ionophore. Excess calcium overcame the inhibitory effect of magnesium. 5. The ionophore did not modify release of catecholamines by induced splanchnic nerve stimulation. 6. The results suggest that the ionophore, like depolarization, introduces calcium into the chromaffin cell to cause release of catecholamines."}
{"id": "PMID:1091731", "title": "Diurnal fluctuations of testosterone and LH in the ram: effect of HCG and gonadotrophin-releasing hormone.", "content": "To examine a possible relationship between the release of LH and increasing testosterone levels, blood samples were collected at 2-hr intervals for 24 hr from six mature rams with indwelling jugular cannulae. Although high levels of LH often preceded increases of testosterone, a consistent time relationship between these two hormones was not observed, nor was a diurnal rhythm established for either hormone. The following day, two rams were each injected intravenously with saline, 500 i.u. HCG, or 100 mug synthetic gonadotrophin-releasing hormone (Gn-RH) respectively. Immediately after the injection, blood was collected every 15 min for approximately 4 hr. After Gn-RH, the plasma LH rose 25- to 50-fold within 12 min and was elevated for more than 3 hr. A twofold rise of plasma testosterone followed this increase of LH and testosterone remained high until the end of the experiment. After HCG treatment, the level of testosterone rose threefold within 30 min and did not decline during the experiment, but there was no effect on the LH level. Saline treatment did not influence levels of LH or testosterone. It was concluded that daily fluctuations of testosterone and LH may occur independently, and no pattern is common to all rams. Treatment with Gn-RH results in a rise in both the testosterone and LH levels, but only testosterone is affected by HCG.", "contents": "Diurnal fluctuations of testosterone and LH in the ram: effect of HCG and gonadotrophin-releasing hormone. To examine a possible relationship between the release of LH and increasing testosterone levels, blood samples were collected at 2-hr intervals for 24 hr from six mature rams with indwelling jugular cannulae. Although high levels of LH often preceded increases of testosterone, a consistent time relationship between these two hormones was not observed, nor was a diurnal rhythm established for either hormone. The following day, two rams were each injected intravenously with saline, 500 i.u. HCG, or 100 mug synthetic gonadotrophin-releasing hormone (Gn-RH) respectively. Immediately after the injection, blood was collected every 15 min for approximately 4 hr. After Gn-RH, the plasma LH rose 25- to 50-fold within 12 min and was elevated for more than 3 hr. A twofold rise of plasma testosterone followed this increase of LH and testosterone remained high until the end of the experiment. After HCG treatment, the level of testosterone rose threefold within 30 min and did not decline during the experiment, but there was no effect on the LH level. Saline treatment did not influence levels of LH or testosterone. It was concluded that daily fluctuations of testosterone and LH may occur independently, and no pattern is common to all rams. Treatment with Gn-RH results in a rise in both the testosterone and LH levels, but only testosterone is affected by HCG."}
{"id": "PMID:1091734", "title": "Somatostatin analogs. Relative importance of the disulfide bridge and of the Ala-Gly side chain for biological activity.", "content": "Four analogs of ovine somatostatin (SRIF, PSOMATOTROPIN RELEASE INhibiting factor), the sequence of which is H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-trp-Lys-Thr-Phe-Thr-Ser-Cys-OH, have been synthesized by the solid-phase methodology. The compounds were assayed and were found to possess the following somatotropin release inhibiting potencies relative to pure synthetic somatostatin in vitro and in vivo, respectively: [Ala3,14]somatostatin, 0.6 and 2.0%; [SMe-Cys3,14]somatostatin, 4 and 0.6%; [NAc-Cys3]somatostatin, 39 and 105%; [des-Ala1-Gly2]somatostatin, 65 and 71%. The dihydrosomatostatin analogs [NAc-Cys3-H2]somatostatin and [des-Ala1Gly2-H2]somatostatin after two purifications by gel filtration were assessed to be at least 80% homogeneous and had respectively 99 and 89% of somatostatin potencied in vivo. Structure-activity relationships are discussed.", "contents": "Somatostatin analogs. Relative importance of the disulfide bridge and of the Ala-Gly side chain for biological activity. Four analogs of ovine somatostatin (SRIF, PSOMATOTROPIN RELEASE INhibiting factor), the sequence of which is H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-trp-Lys-Thr-Phe-Thr-Ser-Cys-OH, have been synthesized by the solid-phase methodology. The compounds were assayed and were found to possess the following somatotropin release inhibiting potencies relative to pure synthetic somatostatin in vitro and in vivo, respectively: [Ala3,14]somatostatin, 0.6 and 2.0%; [SMe-Cys3,14]somatostatin, 4 and 0.6%; [NAc-Cys3]somatostatin, 39 and 105%; [des-Ala1-Gly2]somatostatin, 65 and 71%. The dihydrosomatostatin analogs [NAc-Cys3-H2]somatostatin and [des-Ala1Gly2-H2]somatostatin after two purifications by gel filtration were assessed to be at least 80% homogeneous and had respectively 99 and 89% of somatostatin potencied in vivo. Structure-activity relationships are discussed."}
{"id": "PMID:1091735", "title": "Cyclopenta[f]isoquinoline derivatives designed to bind specifically to native deoxyribonucleic acid. 2. Synthesis of 6-carbamylmethyl-8-methyl-7(5)H-cyclopenta[f]isoquinolin-3(2H)-one and its interaction with deoxyribonucleic acids and poly(deoxyribonucleotides).", "content": "3-Ethoxy-8-methyl-5,6-dihydro-7H-cyclopenta[f]isoquinolin-5-one (2) was converted to 6-carbethoxymethyl-3-ethoxy-8-methyl-5,6-dihydro-7H-cyclopenta[f]isoquinolin-5-one (6) through an oxalyl derivative. Treatment of 6 with ammonia gave the corresponding amide 7 which on sodium borohydride reduction and subsequent dehydration yielded 6-carbamylmethyl-3-ethoxy-8-methyl-7(5)H-cyclopenta[f]isoquinoline (9). The analogous ester 10 was similarly obtained from 6. Numerous attempts to dealkylate the 3-ethoxy group of 9 or 10 failed. However, 6 coould easily be dealkylated on heating with 25% hydrochloric acid in a sealed tube.The ester, 6-carbethoxymethyl-8-methyl-5,6-dihydro-7H-cyclopenta[f]isoquinoline-3(2H),5-dione (11), so obtained was converted to the corresponding amide 12 which on reduction with sodium borohydride and subsequent dehydration afforded the desired compound, 6-car-bamylmethyl-8-methyl-7(5)H-cyclopental[f]isoquinolin-3-(2H)-one (1). 1 was found to be mildly cytotoxic againstL5178Y mouse leukemia cells in culture.1 was also found to bind to native calf thymus DNA. 1 inhibited RNA synthesis by a DNA-dependent RNA polymerase and a higher inhibition of RNA synthesis was observed when poly(dG-dC) was used as a template than when poly(dA-dT) was used. A significant increase of thermal transition temperature of calf thymus DNA and poly(dG)-poly(dC) was observed in the presence of 1. The accumulated evidence demonstrates that 1 interacts weakly with calf thymus DNA and interacts preferentially with poly(deoxyribonucleotides)-containing GC pairs.", "contents": "Cyclopenta[f]isoquinoline derivatives designed to bind specifically to native deoxyribonucleic acid. 2. Synthesis of 6-carbamylmethyl-8-methyl-7(5)H-cyclopenta[f]isoquinolin-3(2H)-one and its interaction with deoxyribonucleic acids and poly(deoxyribonucleotides). 3-Ethoxy-8-methyl-5,6-dihydro-7H-cyclopenta[f]isoquinolin-5-one (2) was converted to 6-carbethoxymethyl-3-ethoxy-8-methyl-5,6-dihydro-7H-cyclopenta[f]isoquinolin-5-one (6) through an oxalyl derivative. Treatment of 6 with ammonia gave the corresponding amide 7 which on sodium borohydride reduction and subsequent dehydration yielded 6-carbamylmethyl-3-ethoxy-8-methyl-7(5)H-cyclopenta[f]isoquinoline (9). The analogous ester 10 was similarly obtained from 6. Numerous attempts to dealkylate the 3-ethoxy group of 9 or 10 failed. However, 6 coould easily be dealkylated on heating with 25% hydrochloric acid in a sealed tube.The ester, 6-carbethoxymethyl-8-methyl-5,6-dihydro-7H-cyclopenta[f]isoquinoline-3(2H),5-dione (11), so obtained was converted to the corresponding amide 12 which on reduction with sodium borohydride and subsequent dehydration afforded the desired compound, 6-car-bamylmethyl-8-methyl-7(5)H-cyclopental[f]isoquinolin-3-(2H)-one (1). 1 was found to be mildly cytotoxic againstL5178Y mouse leukemia cells in culture.1 was also found to bind to native calf thymus DNA. 1 inhibited RNA synthesis by a DNA-dependent RNA polymerase and a higher inhibition of RNA synthesis was observed when poly(dG-dC) was used as a template than when poly(dA-dT) was used. A significant increase of thermal transition temperature of calf thymus DNA and poly(dG)-poly(dC) was observed in the presence of 1. The accumulated evidence demonstrates that 1 interacts weakly with calf thymus DNA and interacts preferentially with poly(deoxyribonucleotides)-containing GC pairs."}
{"id": "PMID:1091736", "title": "Chemistry of cephalosporin antibiotics. 30. 3-methoxy- and 3-halo-3-cephems.", "content": "The exo-methylene group in esters of 7-acylamido- and 7-amino-3-methylenecephams was ozonized to give 3-hydroxy-3-cephems. Conditions are described to effect a selective N-acylation of a 3-hydroxy-3-cephem nucleus ester. Vilsmeier reagents converted 7-acylamido-3-hydroxy compounds to 3-halo-3-cephem derivatives. Diazomethane converted the 3-hydroxy compounds to 3-methoxy-3-cephem derivatives. Removal of the ester-protecting group at the C4-carboxyl afforded a select group of cephalosporins with direct halo and methoxy substitution at C3. A number of these compounds are potent antibiotics.", "contents": "Chemistry of cephalosporin antibiotics. 30. 3-methoxy- and 3-halo-3-cephems. The exo-methylene group in esters of 7-acylamido- and 7-amino-3-methylenecephams was ozonized to give 3-hydroxy-3-cephems. Conditions are described to effect a selective N-acylation of a 3-hydroxy-3-cephem nucleus ester. Vilsmeier reagents converted 7-acylamido-3-hydroxy compounds to 3-halo-3-cephem derivatives. Diazomethane converted the 3-hydroxy compounds to 3-methoxy-3-cephem derivatives. Removal of the ester-protecting group at the C4-carboxyl afforded a select group of cephalosporins with direct halo and methoxy substitution at C3. A number of these compounds are potent antibiotics."}
{"id": "PMID:1091737", "title": "Pseudosymmetry in the structure of luteinizing hormone-releasing hormone. Studies on a series of novel analogs.", "content": "Pseudosymmetry in the LH-RH structure is described. Eleven analogs of LH-RH (SMALLER THAN Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2) have been synthesized by the fragment condensation method and the repetitive excess mixed anhydride method. Multiple substitutions have been made in the LH-RH sequence, which retain the pseudosymmetry of the LH-RH molecule, while presenting fewer problems of synthesis than the corresponding residues in the natural decapeptide. Thus Trp3, Ser4, Tyr5, Gly6, Leu7, and Arg8 residues were replaced by amino acids having similar properties to the residues that they replace. In all but one of the peptides the Gly10-NH2 residue was replaced by ethylamide, while in the remaining peptide, 1-methyl-5-aminomethyltetrazole (AMT-Me) was substituted at position 10. The compounds were assayed in vitro and in vivo. The following analogs had in vivo and in vitro activities in the range 1-28 percent relative to LH-RH: I, smaller than Glu-His-Phe-Ala-Tyr-Gly-Leu-Arg-Pro-NHEt; II, smaller than Glu-His-Phe-Gly-Tyr-Gly-Leu-Arg-Pro-NHEt; VII, smaller than Glu-His-Phe-Ala-Tyr-Gly-Phe-Arg-Pro-NHEt; IX, smaller than Glu-His-Phe-Ala-Tyr-D-Ala-Leu-Arg-Pro-NHEt; XI, smaller than Glu-His-Phe-Gly-Tyr-Gly-Leu-Arg-Pro-AMT-Me.", "contents": "Pseudosymmetry in the structure of luteinizing hormone-releasing hormone. Studies on a series of novel analogs. Pseudosymmetry in the LH-RH structure is described. Eleven analogs of LH-RH (SMALLER THAN Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2) have been synthesized by the fragment condensation method and the repetitive excess mixed anhydride method. Multiple substitutions have been made in the LH-RH sequence, which retain the pseudosymmetry of the LH-RH molecule, while presenting fewer problems of synthesis than the corresponding residues in the natural decapeptide. Thus Trp3, Ser4, Tyr5, Gly6, Leu7, and Arg8 residues were replaced by amino acids having similar properties to the residues that they replace. In all but one of the peptides the Gly10-NH2 residue was replaced by ethylamide, while in the remaining peptide, 1-methyl-5-aminomethyltetrazole (AMT-Me) was substituted at position 10. The compounds were assayed in vitro and in vivo. The following analogs had in vivo and in vitro activities in the range 1-28 percent relative to LH-RH: I, smaller than Glu-His-Phe-Ala-Tyr-Gly-Leu-Arg-Pro-NHEt; II, smaller than Glu-His-Phe-Gly-Tyr-Gly-Leu-Arg-Pro-NHEt; VII, smaller than Glu-His-Phe-Ala-Tyr-Gly-Phe-Arg-Pro-NHEt; IX, smaller than Glu-His-Phe-Ala-Tyr-D-Ala-Leu-Arg-Pro-NHEt; XI, smaller than Glu-His-Phe-Gly-Tyr-Gly-Leu-Arg-Pro-AMT-Me."}
{"id": "PMID:1091740", "title": "Specific and nonspecific antitumor immunity. I. Description of an in vitro assay based on inhibition of DNA synthesis in tumor cells.", "content": "As in vitro assay of cell-mediated antitumor immunity based on the inhibition of tumor cell DNA synthesis (IDS) was devised. It was reasoned that both cytolytic and cytostatic antitumor effects could be measured in a quantitative yet generalized manner with this approach. By the use of microculture techniques and simplified methods for the determination of tritiated-thymidine incorporation by all cells in individual cultures, normal and immune effector (E) cell populations were compared in their ability to inhibit the DNA synthesis of a fixed initial number of various target tumor cells. Doubling dilutions of E cells were used to titrate the antitumor effects of normal and immune cells at many E/T (effector to target) ratios. Under conditions of alloimmunization, significant immunologically specific IDS effects could routinely be detected at an E/T ratio of less than 1 or 0.1:1, and under certain conditions at 1:100 or less. Results were highly reproducible with respect to the individual E cell donors, replicate cultures, and repeat experiments. The effects were proportional to visually determined cell destruction and independent of obvious culture artifacts. The IDS method was compared with the 51Cr release technique under various experimental conditions. The results demonstrated that decreases in E/T ratio and/or the addition of excess nonimmune cells to immune effector populations had a similar effect in both assays, which was to decrease the magnitude of the immune cell activity. Addition of excess normal cells reduced the activity of immune cells to a level below that of an equal number of immune cells tested at the same E/T ratio without added nonimmune cells. Both assays detected primarily a T lymphocyte-mediated lytic event when effectors generated by the described alloimmunizations were used. The IDS assay also detected a weak non-T-cell activity in anti-theta plus complement-treated alloimmune spleen. The possibility that this represented antibody-dependent cell-mediated cytotoxicity was raised by the finding that normal spleen ceels plus antitarget antibody had significant activity in the IDS system. The sensitivities of the two methods were compared and the potential of the IDS method was evaluated.", "contents": "Specific and nonspecific antitumor immunity. I. Description of an in vitro assay based on inhibition of DNA synthesis in tumor cells. As in vitro assay of cell-mediated antitumor immunity based on the inhibition of tumor cell DNA synthesis (IDS) was devised. It was reasoned that both cytolytic and cytostatic antitumor effects could be measured in a quantitative yet generalized manner with this approach. By the use of microculture techniques and simplified methods for the determination of tritiated-thymidine incorporation by all cells in individual cultures, normal and immune effector (E) cell populations were compared in their ability to inhibit the DNA synthesis of a fixed initial number of various target tumor cells. Doubling dilutions of E cells were used to titrate the antitumor effects of normal and immune cells at many E/T (effector to target) ratios. Under conditions of alloimmunization, significant immunologically specific IDS effects could routinely be detected at an E/T ratio of less than 1 or 0.1:1, and under certain conditions at 1:100 or less. Results were highly reproducible with respect to the individual E cell donors, replicate cultures, and repeat experiments. The effects were proportional to visually determined cell destruction and independent of obvious culture artifacts. The IDS method was compared with the 51Cr release technique under various experimental conditions. The results demonstrated that decreases in E/T ratio and/or the addition of excess nonimmune cells to immune effector populations had a similar effect in both assays, which was to decrease the magnitude of the immune cell activity. Addition of excess normal cells reduced the activity of immune cells to a level below that of an equal number of immune cells tested at the same E/T ratio without added nonimmune cells. Both assays detected primarily a T lymphocyte-mediated lytic event when effectors generated by the described alloimmunizations were used. The IDS assay also detected a weak non-T-cell activity in anti-theta plus complement-treated alloimmune spleen. The possibility that this represented antibody-dependent cell-mediated cytotoxicity was raised by the finding that normal spleen ceels plus antitarget antibody had significant activity in the IDS system. The sensitivities of the two methods were compared and the potential of the IDS method was evaluated."}
{"id": "PMID:1091741", "title": "Specific and nonspecific antitumor immunity. II. Macrophage-mediated nonspecific effector activity induced by BCG and similar agents.", "content": "The recently described inhibition of DNA synthesis (IDS) assay, which measures antitumor effector (E) cell function by the quantitation of decreases in tritiated thymidine incorporation of target tumor cells, was used to analyze the nonspecific effector activity of peritoneal exudate cells (PEC) from mice infected intraperitoneally with BCG. These PEC could inhibit growth of and then kill all tumor target (effector to target) cells tested at E/T ratios as low as 1:1. This activity was not due to alterations in media, nor could any activity be shown for cell-free supernatants prepared from active cells. The principal cell type mediating this effector function was the \"activated\" macrophage-no activity was found in lymphoid or polymorphonuclear leukocytes. Direct effector to target contact was necessary for the cytotoxic reaction. The time course of these effects and comparative 51Cr release data were reported. The removal of the adherent nonspecific effectors from PEC of mice immunized to both BCG and a specific syngeneic tumor revealed a specific cytotoxicity of the remaining lymphoid cells. These results indicated that nonspecific effector activity by \"activated\" macrophages induced by BCG infection could be a potent antitumor mechanism, at least in vitro, and the IDS assay provided an accurate, reproducible, and quantitative method for measurement of the function of such E cells.", "contents": "Specific and nonspecific antitumor immunity. II. Macrophage-mediated nonspecific effector activity induced by BCG and similar agents. The recently described inhibition of DNA synthesis (IDS) assay, which measures antitumor effector (E) cell function by the quantitation of decreases in tritiated thymidine incorporation of target tumor cells, was used to analyze the nonspecific effector activity of peritoneal exudate cells (PEC) from mice infected intraperitoneally with BCG. These PEC could inhibit growth of and then kill all tumor target (effector to target) cells tested at E/T ratios as low as 1:1. This activity was not due to alterations in media, nor could any activity be shown for cell-free supernatants prepared from active cells. The principal cell type mediating this effector function was the \"activated\" macrophage-no activity was found in lymphoid or polymorphonuclear leukocytes. Direct effector to target contact was necessary for the cytotoxic reaction. The time course of these effects and comparative 51Cr release data were reported. The removal of the adherent nonspecific effectors from PEC of mice immunized to both BCG and a specific syngeneic tumor revealed a specific cytotoxicity of the remaining lymphoid cells. These results indicated that nonspecific effector activity by \"activated\" macrophages induced by BCG infection could be a potent antitumor mechanism, at least in vitro, and the IDS assay provided an accurate, reproducible, and quantitative method for measurement of the function of such E cells."}
{"id": "PMID:1091742", "title": "Differential cytotoxicity of tumorigenic and nontumorigenic strain-2 guinea pig cells as mediated by syngeneic phytohemagglutinin-stimulated peritoneal exudate cells.", "content": "Phytohemagglutinin (PHA)-stimulated peritoneal exudate (PE) cells from strain-2 guinea pigs were more cytotoxic in culture to syngeneic tumorigenic cells than to syngeneic nontumorigenic cells. Cytotoxicity was measured by the release of 3H-thymidine from prelabeled target cells. Tumor-producing guinea pig fetal cells transformed in culture by chemical carcinogen released up to 70 percent of their label in the presence of PHA-stimulated PE cells. Non-tumor-producing cells, regardless of their morphologic characteristics, were less affected by PHA-stimulated PE cells. Nontumorigenic cultures included untreated early passage and long-term cultures previously treated with a carcinogenic or noncarcinogenic chemical. Differential cytotoxicity to tumorigenic cultures were best distinguished from nontumorigenic cultures when 0.5-1 times 10-6 PE cells and 50-100 mug PHA were incubated with 1 times 10-4 3H-thymidine-labeled target cells for 48 hours.", "contents": "Differential cytotoxicity of tumorigenic and nontumorigenic strain-2 guinea pig cells as mediated by syngeneic phytohemagglutinin-stimulated peritoneal exudate cells. Phytohemagglutinin (PHA)-stimulated peritoneal exudate (PE) cells from strain-2 guinea pigs were more cytotoxic in culture to syngeneic tumorigenic cells than to syngeneic nontumorigenic cells. Cytotoxicity was measured by the release of 3H-thymidine from prelabeled target cells. Tumor-producing guinea pig fetal cells transformed in culture by chemical carcinogen released up to 70 percent of their label in the presence of PHA-stimulated PE cells. Non-tumor-producing cells, regardless of their morphologic characteristics, were less affected by PHA-stimulated PE cells. Nontumorigenic cultures included untreated early passage and long-term cultures previously treated with a carcinogenic or noncarcinogenic chemical. Differential cytotoxicity to tumorigenic cultures were best distinguished from nontumorigenic cultures when 0.5-1 times 10-6 PE cells and 50-100 mug PHA were incubated with 1 times 10-4 3H-thymidine-labeled target cells for 48 hours."}
{"id": "PMID:1091743", "title": "In vivo and in vitro tests of inhibitory effect of progesterone on cell-mediated immunity in rats bearing a syngeneic uterine adenocarcinoma.", "content": "Growth inhibition or stimulation of target adenocarcinoma cells in rats sensitized with spleen cells from syngeneic tumor-bearing rats was significantly suppressed in colony inhibition assays when the spleen cells were treated in vitro with 0.8 mug progesterone or more/ml medium. In addition, when tumor-bearing rats were treated with 1 mg medroxyprogesterone acetate weekly in vivo, the inhibiting action of the spleen cells from rats with regressed tumors was also suppressed. Progesterone thus suppressed immune spleen cells in vivo and in vitro.", "contents": "In vivo and in vitro tests of inhibitory effect of progesterone on cell-mediated immunity in rats bearing a syngeneic uterine adenocarcinoma. Growth inhibition or stimulation of target adenocarcinoma cells in rats sensitized with spleen cells from syngeneic tumor-bearing rats was significantly suppressed in colony inhibition assays when the spleen cells were treated in vitro with 0.8 mug progesterone or more/ml medium. In addition, when tumor-bearing rats were treated with 1 mg medroxyprogesterone acetate weekly in vivo, the inhibiting action of the spleen cells from rats with regressed tumors was also suppressed. Progesterone thus suppressed immune spleen cells in vivo and in vitro."}
{"id": "PMID:1091744", "title": "Mouse mononuclear cell chemotaxis: description of system.", "content": "The mononuclear cell chemotaxis assay was adapted for use with normal mouse peritoneal cells. Mouse peritoneal macrophages responded well to endotoxinactivated mouse serum and to chemotactic factors produced by mouse spleen cell cultures stimulated with mitogens or specific antigen. The assay was quantitative, reproducible, and applicable to several mouse strains.", "contents": "Mouse mononuclear cell chemotaxis: description of system. The mononuclear cell chemotaxis assay was adapted for use with normal mouse peritoneal cells. Mouse peritoneal macrophages responded well to endotoxinactivated mouse serum and to chemotactic factors produced by mouse spleen cell cultures stimulated with mitogens or specific antigen. The assay was quantitative, reproducible, and applicable to several mouse strains."}
{"id": "PMID:1091747", "title": "Self-screening for significant bacteriuria. Evaluation of dip-strip combination nitrite/culture test.", "content": "A self-administered dip-strip device was used to screen for urinary tract infection in female subjects. Fifty-two cases of significant persistent bacteriuria were detected among 2,250 subjects by simultaneous use of pourplate quantitative cultures. The dip-strip culture test had good reliability in detecting Gram-negative organisms. Repeated nitrite tests, performed on first-morning specimens and read by the patient, also had good reliability in detecting Gram-negative bacteria. No false-positive test occurred. The device is effective in screening out contaminants, thus decreasing the need for repeated testing.", "contents": "Self-screening for significant bacteriuria. Evaluation of dip-strip combination nitrite/culture test. A self-administered dip-strip device was used to screen for urinary tract infection in female subjects. Fifty-two cases of significant persistent bacteriuria were detected among 2,250 subjects by simultaneous use of pourplate quantitative cultures. The dip-strip culture test had good reliability in detecting Gram-negative organisms. Repeated nitrite tests, performed on first-morning specimens and read by the patient, also had good reliability in detecting Gram-negative bacteria. No false-positive test occurred. The device is effective in screening out contaminants, thus decreasing the need for repeated testing."}
{"id": "PMID:1091748", "title": "Treatment of persons exposed to rabies.", "content": "Several clinical and epidemiologic criteria have been formulated by the Center for Disease Control in advising physicians on the treatment of persons exposed to possibly rabit animals. When deciding whether or not to institute rabies prophylaxis, the physician must consider (1) the type of exposure, (2) the animal species involved in the exposure and the regional epidemiology of rabies, (3) the circumstances surrounding the exposure, and (4) the treatment alternatives and complications.", "contents": "Treatment of persons exposed to rabies. Several clinical and epidemiologic criteria have been formulated by the Center for Disease Control in advising physicians on the treatment of persons exposed to possibly rabit animals. When deciding whether or not to institute rabies prophylaxis, the physician must consider (1) the type of exposure, (2) the animal species involved in the exposure and the regional epidemiology of rabies, (3) the circumstances surrounding the exposure, and (4) the treatment alternatives and complications."}
{"id": "PMID:1091749", "title": "Psoas abscess localization by gallium scan in aplastic anemia.", "content": "Gallium 67 scanning is an effective method of detecting inflammatory lesions, especially abscesses. A 10-year-old boy with aplastic anemia and severe leukopenia and granulocytopenia had a psoas abscess diagnosed by gallium scan. The patient died with Candida spesis 18 days after bone marrow transplantation. At autopsy, a chronic psoas abscess with Candida was found. The gallium scan offers a clinically effective and noninvasive means of evaluating suspected infection in the granulocytopenic patient.", "contents": "Psoas abscess localization by gallium scan in aplastic anemia. Gallium 67 scanning is an effective method of detecting inflammatory lesions, especially abscesses. A 10-year-old boy with aplastic anemia and severe leukopenia and granulocytopenia had a psoas abscess diagnosed by gallium scan. The patient died with Candida spesis 18 days after bone marrow transplantation. At autopsy, a chronic psoas abscess with Candida was found. The gallium scan offers a clinically effective and noninvasive means of evaluating suspected infection in the granulocytopenic patient."}
{"id": "PMID:1091750", "title": "Lymphocytic choriomeningitis virus in man. Serologic evidence of association with pet hamsters;.", "content": "Laboratory evidence of recent or current lymphocytic choriomeningitis (LCM) virus infection was obtained in 60 patients. Twelve had diagnosis of central nervous system (CNS) infection: four of meningoencephalitis and eight of meningitis. Thirty-four patients had a grippe-like syndrome. Fifty-nine had had contact with pet hamsters. All of the 24 patients whose pets were studied had been exposed to one or more hamsters with serologic evidence of past LCM virus infection. The data implicate pet hamsters as a source of LCM in man. A continuous effective control of LCM virus in pet hamsters appears impractical. At present, the only feasible way to prevent further cases is the physician's special attention to the possibility of rodent contacts of patients with CNS disease and early laboratory confirmation of suspected cases of human LCM virus infections.", "contents": "Lymphocytic choriomeningitis virus in man. Serologic evidence of association with pet hamsters;. Laboratory evidence of recent or current lymphocytic choriomeningitis (LCM) virus infection was obtained in 60 patients. Twelve had diagnosis of central nervous system (CNS) infection: four of meningoencephalitis and eight of meningitis. Thirty-four patients had a grippe-like syndrome. Fifty-nine had had contact with pet hamsters. All of the 24 patients whose pets were studied had been exposed to one or more hamsters with serologic evidence of past LCM virus infection. The data implicate pet hamsters as a source of LCM in man. A continuous effective control of LCM virus in pet hamsters appears impractical. At present, the only feasible way to prevent further cases is the physician's special attention to the possibility of rodent contacts of patients with CNS disease and early laboratory confirmation of suspected cases of human LCM virus infections."}
{"id": "PMID:1091760", "title": "Difficulties in obtaining kidneys from potential postmortem donors.", "content": "Disease outcome and kidney disposition of 86 patients referred as potential postmortem kidney donors were followed up over a one-year period to determine factors that led to \"organ wastage.\" Six patients died before fulfilling the electorencephalographic criteria for death; another six were referred too late, leaving insufficient time to contact the relatives; and in 16 patients, permission was refused by the next of kin. In eight cases, no relatives could be traced and, finally, 14 patients died wtthout fulfilling our criteria of acceptability. Organs were obtained from only 22 donors--25.5% of the total referrals. Any data presenting the number of potential postmortem kidney donors must be considered in thp light of factors incorporating the ease or difficulty with which kidney grafts can be obtained. An awareness of these factors is necessary for maximum utilization of potential kidney donors.", "contents": "Difficulties in obtaining kidneys from potential postmortem donors. Disease outcome and kidney disposition of 86 patients referred as potential postmortem kidney donors were followed up over a one-year period to determine factors that led to \"organ wastage.\" Six patients died before fulfilling the electorencephalographic criteria for death; another six were referred too late, leaving insufficient time to contact the relatives; and in 16 patients, permission was refused by the next of kin. In eight cases, no relatives could be traced and, finally, 14 patients died wtthout fulfilling our criteria of acceptability. Organs were obtained from only 22 donors--25.5% of the total referrals. Any data presenting the number of potential postmortem kidney donors must be considered in thp light of factors incorporating the ease or difficulty with which kidney grafts can be obtained. An awareness of these factors is necessary for maximum utilization of potential kidney donors."}
{"id": "PMID:1091761", "title": "Decisive factors in the tolbutamide controversy.", "content": "No increased mortality trend attributable to tolbutamide is shown by an analysis of variance on logit-transformed data from the University Group Diabetes Program (UGDP) study. The UGDP's controversial finding of an increased rate with mortality subgrouped by \"cardiovascular\" causes is confirmed by the Biometric Committee's report, with reservations that failed to include overriding decisive factors. The basic problem is that inspected data set up the hypothesis (the increased cardiovascular mortality), and that the same data were used to test the hypothesis, so that resulting probability values no longer have the usual meaning. The problem was compounded by multiple testing of the data without adjusting the probability levels. When cardiovascular deaths were redefined as myocardial infarcts and sudden deaths, in an attempt to test a proposed etiologic inotropic hypothesis, no significant increase in cardiovascular mortality was found.", "contents": "Decisive factors in the tolbutamide controversy. No increased mortality trend attributable to tolbutamide is shown by an analysis of variance on logit-transformed data from the University Group Diabetes Program (UGDP) study. The UGDP's controversial finding of an increased rate with mortality subgrouped by \"cardiovascular\" causes is confirmed by the Biometric Committee's report, with reservations that failed to include overriding decisive factors. The basic problem is that inspected data set up the hypothesis (the increased cardiovascular mortality), and that the same data were used to test the hypothesis, so that resulting probability values no longer have the usual meaning. The problem was compounded by multiple testing of the data without adjusting the probability levels. When cardiovascular deaths were redefined as myocardial infarcts and sudden deaths, in an attempt to test a proposed etiologic inotropic hypothesis, no significant increase in cardiovascular mortality was found."}
{"id": "PMID:1091770", "title": "[Extracorporeal attachment of the allo-and xenokidneys to recipients' body for temporary decrease of the level of antibodies and complement activity].", "content": "Data given in our investigation put questions important for clinical aspects of transplantation on efficacy of perfusional immunosorption for subsequent kidney transplantation with a temporarily reduced level of preexistent antibodies, on the character of evolution of the secondary response under the same conditions, on the level of immunodepression required in treating such patients etc.", "contents": "[Extracorporeal attachment of the allo-and xenokidneys to recipients' body for temporary decrease of the level of antibodies and complement activity]. Data given in our investigation put questions important for clinical aspects of transplantation on efficacy of perfusional immunosorption for subsequent kidney transplantation with a temporarily reduced level of preexistent antibodies, on the character of evolution of the secondary response under the same conditions, on the level of immunodepression required in treating such patients etc."}
{"id": "PMID:1091772", "title": "[Hemodynamics in patients with circulatory insufficiency depending on the activity of the rheumatic process].", "content": "In 384 rheumatic patients, including 344 with mitral defect of the heart central hemodynamics, phasic structure of the left ventricle systole and renal hemodynamics were studied. With the help of the sought out algorythm and the computer technique qualitative information on the activity of the rheumatic process, expressed in terms of plausability proportions for each patient, was obtained. This was followed by a search for a correlation between the values of these proportions and the parameters of the cardiac function. An inverse correlation was found only relative to the stroke index and the ejection rate and merely in patients with mitral defect and circulatory insufficiency of the IIB-III stage. No essential difference was found between the values characterizing the hemodynamics, the duration of the left ventricle systole phases recorded in the groups of patients with active and inactive rheumatism, but at equal stages of ciruclatory insufficiency.", "contents": "[Hemodynamics in patients with circulatory insufficiency depending on the activity of the rheumatic process]. In 384 rheumatic patients, including 344 with mitral defect of the heart central hemodynamics, phasic structure of the left ventricle systole and renal hemodynamics were studied. With the help of the sought out algorythm and the computer technique qualitative information on the activity of the rheumatic process, expressed in terms of plausability proportions for each patient, was obtained. This was followed by a search for a correlation between the values of these proportions and the parameters of the cardiac function. An inverse correlation was found only relative to the stroke index and the ejection rate and merely in patients with mitral defect and circulatory insufficiency of the IIB-III stage. No essential difference was found between the values characterizing the hemodynamics, the duration of the left ventricle systole phases recorded in the groups of patients with active and inactive rheumatism, but at equal stages of ciruclatory insufficiency."}
{"id": "PMID:1091773", "title": "[Additional gadget for new techniques of artifical respiration with the Bennett repirator PR 2, particulary suitable for use in children (author's transl)].", "content": "A new gadget for the Bennett PR 2 repirator is described which in modified form can also be used with other types of apparatus. It makes possible new techniques of artificial respiration like IPPB with PEEP or compensated PEEP, the gasp respiration with CPAP or CPAP by itself, in a simple way. In neonates one may do without the often problematical assisted respiration when weaning from the respirator. Further it is now possible to achieve sufficient warming and humidification of the gas mixture by using an auxiliary gas flow in the expiratory phase.", "contents": "[Additional gadget for new techniques of artifical respiration with the Bennett repirator PR 2, particulary suitable for use in children (author's transl)]. A new gadget for the Bennett PR 2 repirator is described which in modified form can also be used with other types of apparatus. It makes possible new techniques of artificial respiration like IPPB with PEEP or compensated PEEP, the gasp respiration with CPAP or CPAP by itself, in a simple way. In neonates one may do without the often problematical assisted respiration when weaning from the respirator. Further it is now possible to achieve sufficient warming and humidification of the gas mixture by using an auxiliary gas flow in the expiratory phase."}
{"id": "PMID:1091774", "title": "Experimental oliguric acute renal failure: protective effects of renomedullary autotransplants.", "content": "An acute circulatory renal failure (ARF) was induced in 18 rabbits by temporary ischemia of the remaining kidney 8 days after unilateral nephrectomy and subcuteaneous autotransplantation of renomedullary tissue.--Mortality in the postischemic course was 50% in treated animals but 100% in the control group (n = 18) without autotransplantation. In the postischemic period plasma urea concentration was significantly lower (p smaller than 0.005) in the surviving transplanted animals and excretion of sodium and water significantly higher (p smaller than 0.005) as compared with the control group. Plasma renin values which were significantly lower than thos of the control(p smaller than 0.005) had decreased significantly even as compared with the initial values. These results indicate that hormonal substances are produced in interstitial cells of renomedullary autotransplants exerting a distinct protective effect against experimental acute renal failure. Decreased plasma renin activity may point to an inhibition of circulating and/or intrarenal renin by lipids originating from the transplants. Changes in sodium and water excretion indicate effects of circulating prostaglandins", "contents": "Experimental oliguric acute renal failure: protective effects of renomedullary autotransplants. An acute circulatory renal failure (ARF) was induced in 18 rabbits by temporary ischemia of the remaining kidney 8 days after unilateral nephrectomy and subcuteaneous autotransplantation of renomedullary tissue.--Mortality in the postischemic course was 50% in treated animals but 100% in the control group (n = 18) without autotransplantation. In the postischemic period plasma urea concentration was significantly lower (p smaller than 0.005) in the surviving transplanted animals and excretion of sodium and water significantly higher (p smaller than 0.005) as compared with the control group. Plasma renin values which were significantly lower than thos of the control(p smaller than 0.005) had decreased significantly even as compared with the initial values. These results indicate that hormonal substances are produced in interstitial cells of renomedullary autotransplants exerting a distinct protective effect against experimental acute renal failure. Decreased plasma renin activity may point to an inhibition of circulating and/or intrarenal renin by lipids originating from the transplants. Changes in sodium and water excretion indicate effects of circulating prostaglandins"}
{"id": "PMID:1091775", "title": "[5-Aminoacridine hydrochloride for fixation of human blood basophils (author's transl)].", "content": "5-aminoacridine hydrochloride (0.4 percent in 50 percent aqueous ethanol) proved to be a useful fixative for the preservation of human blood basophils pretreated with methanol-formaldehyde (9:1 V/V). Leaching of the water-soluble basophil granules or other artificial cell alterations common to fixatives in current use were not observed. The organic compound used did not induce any chemographic artifacts in nuclear tracers, in contrast to most of the other fixatives hitherto applied. 5-aminoacridine hydrochloride is therefore particularly suitable for autoradiography of the human blood basophils.", "contents": "[5-Aminoacridine hydrochloride for fixation of human blood basophils (author's transl)]. 5-aminoacridine hydrochloride (0.4 percent in 50 percent aqueous ethanol) proved to be a useful fixative for the preservation of human blood basophils pretreated with methanol-formaldehyde (9:1 V/V). Leaching of the water-soluble basophil granules or other artificial cell alterations common to fixatives in current use were not observed. The organic compound used did not induce any chemographic artifacts in nuclear tracers, in contrast to most of the other fixatives hitherto applied. 5-aminoacridine hydrochloride is therefore particularly suitable for autoradiography of the human blood basophils."}
{"id": "PMID:1091776", "title": "Technic for serial bone sampling in canine skeletal labeling studies.", "content": "A technic was described for repetitive bone sampling in beagle dogs. The procedure has provided ideal samples for quantitative histologic technics following 135 biopsies.", "contents": "Technic for serial bone sampling in canine skeletal labeling studies. A technic was described for repetitive bone sampling in beagle dogs. The procedure has provided ideal samples for quantitative histologic technics following 135 biopsies."}
{"id": "PMID:1091777", "title": "Colitis cystica profunda in rhesus monkeys.", "content": "Spontaneously occurring colitis cystica profunda, characterized by the presence of non-neoplastic glands and mucin-containing cysts in the submucosa of the large intestine, was observed post mortem in 4 of 28 rhesus monkeys (Macaca mulatta) but in none of 20 squirrel monkeys (Saimiri sciuresu) necropsied during the same period of time. An analogous lesion in the stomach was present in 2 of the monkeys with the colonic condition. The submucosal glands and cysts resulted from extension of the mucosa through the muscularis mucosae, and inflammation was considered to have played a primary role in this process. The cause of the intestinal inflammation was not determined, and there was no known exposure to toxic chemicals, including pesticides and polychlorinated biphenyls. Colitis cystica profunda affecting man and other animals has been described infrequently in the literature, but the importance of differentiating it from intestinal adenocarcinoma has been emphasized.", "contents": "Colitis cystica profunda in rhesus monkeys. Spontaneously occurring colitis cystica profunda, characterized by the presence of non-neoplastic glands and mucin-containing cysts in the submucosa of the large intestine, was observed post mortem in 4 of 28 rhesus monkeys (Macaca mulatta) but in none of 20 squirrel monkeys (Saimiri sciuresu) necropsied during the same period of time. An analogous lesion in the stomach was present in 2 of the monkeys with the colonic condition. The submucosal glands and cysts resulted from extension of the mucosa through the muscularis mucosae, and inflammation was considered to have played a primary role in this process. The cause of the intestinal inflammation was not determined, and there was no known exposure to toxic chemicals, including pesticides and polychlorinated biphenyls. Colitis cystica profunda affecting man and other animals has been described infrequently in the literature, but the importance of differentiating it from intestinal adenocarcinoma has been emphasized."}
{"id": "PMID:1091778", "title": "A report of fourteen spontaneous tumors of the guinea pig.", "content": "Fourteen guinea pig tumors were received by the Purdue University Animal Disease Diagnostic Laboratories over a period of 5 yr. Among them were 2 mammary gland adenocarcinomas, a mammary gland adenoma, a malignant mixed tumor of the mammary gland, a Schwannoma, 2 limpomas, an inflammatory polyp of the ear canal, a uterine fibroma, 3 trichoepitheliomas, an undifferentiated carcinoma, and a histiocytic lymphosarcoma. The pathologic features of these neoplasms were presented in this report.", "contents": "A report of fourteen spontaneous tumors of the guinea pig. Fourteen guinea pig tumors were received by the Purdue University Animal Disease Diagnostic Laboratories over a period of 5 yr. Among them were 2 mammary gland adenocarcinomas, a mammary gland adenoma, a malignant mixed tumor of the mammary gland, a Schwannoma, 2 limpomas, an inflammatory polyp of the ear canal, a uterine fibroma, 3 trichoepitheliomas, an undifferentiated carcinoma, and a histiocytic lymphosarcoma. The pathologic features of these neoplasms were presented in this report."}
{"id": "PMID:1091779", "title": "Hexachlorophene retinopathy in rats.", "content": "The primary effect of hexachlorophene on rat retina is disruption of the disc membranes of photoreceptor outer segments and irreversible destruction of their cell bodies. The neurons of the ganglion cell layer of the retina also become affected, probably secondary to optic nerve lesions present in hexachlorophene intoxication. The early and probably reversible changes of the discs are vacuolation and vesiculotubular degeneration. Vacuolation in the discs results from separation of their inner aspects. This lesion is analogous to the separation of myelin lamellae at the intraperiod line, also produced by hexachlorophene.", "contents": "Hexachlorophene retinopathy in rats. The primary effect of hexachlorophene on rat retina is disruption of the disc membranes of photoreceptor outer segments and irreversible destruction of their cell bodies. The neurons of the ganglion cell layer of the retina also become affected, probably secondary to optic nerve lesions present in hexachlorophene intoxication. The early and probably reversible changes of the discs are vacuolation and vesiculotubular degeneration. Vacuolation in the discs results from separation of their inner aspects. This lesion is analogous to the separation of myelin lamellae at the intraperiod line, also produced by hexachlorophene."}
{"id": "PMID:1091780", "title": "Characteristic immunohistochemical and ultrastructural glomerular lesions in Nude mice.", "content": "Kidney glomerular lesions were studied by light microscopy and immunofluorescence in 17 congenitally athymic Nude mice and 25 of their phenotypically normal littermates. The 3-month-old mice had glomerular deposits of total IgG, IgG2, IgM, and C3, but IgG1 was consistently absent and IgA was present in only 30 per cent of cases. Conversely, the 6-month-old littermates showed no deposits. However, the 9- and 12-month-old littermates had glomerular deposits of all of the immunoglobulins and C3. Kidney tissue from five Nude mice was studied by electron microscopy. In three of these five mice the mesangial and subendothelial deposits contained characteristic double layered lamellar structures approximately 130 A wide. Antinuclear antibodies were found in the serum of 3 or 10 Nude mice and 1 of 12 littermates. Different hypotheses on the origin of the characteristic lamellar structures are proposed. The significance of the early development of autoimmunity and glomerular lesions in Nude mice is discussed.", "contents": "Characteristic immunohistochemical and ultrastructural glomerular lesions in Nude mice. Kidney glomerular lesions were studied by light microscopy and immunofluorescence in 17 congenitally athymic Nude mice and 25 of their phenotypically normal littermates. The 3-month-old mice had glomerular deposits of total IgG, IgG2, IgM, and C3, but IgG1 was consistently absent and IgA was present in only 30 per cent of cases. Conversely, the 6-month-old littermates showed no deposits. However, the 9- and 12-month-old littermates had glomerular deposits of all of the immunoglobulins and C3. Kidney tissue from five Nude mice was studied by electron microscopy. In three of these five mice the mesangial and subendothelial deposits contained characteristic double layered lamellar structures approximately 130 A wide. Antinuclear antibodies were found in the serum of 3 or 10 Nude mice and 1 of 12 littermates. Different hypotheses on the origin of the characteristic lamellar structures are proposed. The significance of the early development of autoimmunity and glomerular lesions in Nude mice is discussed."}
{"id": "PMID:1091783", "title": "The expressive portion of the NSST compared to a spontaneous language sample.", "content": "This study was undertaken to determine the relationship between syntactic structures produced on the expressive portion of the NSST and those produced in a spontaneous language sample. The NSST was administered to 12 children previously diagnosed as delayed in language. In addition, spontaneous language samples were collected by a speech clinician and by the child's mother. The children ranged in age from four years one month to five years 11 months. We found that 30 percent of those syntactic structures incorrectly produced on the NSST were correctly produced spontaneously in the language sample. Furthermore, the sample obtained by the clinician was significantly richer in terms of number of structures produced correctly than the sample collected by the mother. Overall results indicate that an item analysis of the expressive portion of the NSST does not present an accurate representation of the child's language performance and therefore cannot be interpreted beyond its stated purpose, namely that of a screening instrument.", "contents": "The expressive portion of the NSST compared to a spontaneous language sample. This study was undertaken to determine the relationship between syntactic structures produced on the expressive portion of the NSST and those produced in a spontaneous language sample. The NSST was administered to 12 children previously diagnosed as delayed in language. In addition, spontaneous language samples were collected by a speech clinician and by the child's mother. The children ranged in age from four years one month to five years 11 months. We found that 30 percent of those syntactic structures incorrectly produced on the NSST were correctly produced spontaneously in the language sample. Furthermore, the sample obtained by the clinician was significantly richer in terms of number of structures produced correctly than the sample collected by the mother. Overall results indicate that an item analysis of the expressive portion of the NSST does not present an accurate representation of the child's language performance and therefore cannot be interpreted beyond its stated purpose, namely that of a screening instrument."}
{"id": "PMID:1091786", "title": "Encephalopathy and fatty infiltration of the viscera (Reye-Johnson syndrome): a 17-year experience.", "content": "Reye-Johnson syndrome was found in 17 patients in a retrospective study of 235 children with acute encephalopathy seen at the Mayo Clinic in the period 1955 through 1971. Eight of the nine patients under 4 years of age developed the syndrome prior to 1968; eight children 10 to 15 years of age acquired the disorder from 1968 through 1971. Seven patients were admitted during the month of February. Prodromal symptoms preceded the encephalopathy in 13 patients. Eight patients had seizures. Progressive deterioration with brainstem signs led to death in 15 patients. Electroencephalographic findings correlated well with the clinical course and eventual outcome. However, no correlation was found between seizure activity and the electroencephalogram. Autopsy findings in 14 cases included cerebral edema, tonsillar herniation, hypoxic neuronal degeneration, and fatty inflitration of the liver and kidneys.", "contents": "Encephalopathy and fatty infiltration of the viscera (Reye-Johnson syndrome): a 17-year experience. Reye-Johnson syndrome was found in 17 patients in a retrospective study of 235 children with acute encephalopathy seen at the Mayo Clinic in the period 1955 through 1971. Eight of the nine patients under 4 years of age developed the syndrome prior to 1968; eight children 10 to 15 years of age acquired the disorder from 1968 through 1971. Seven patients were admitted during the month of February. Prodromal symptoms preceded the encephalopathy in 13 patients. Eight patients had seizures. Progressive deterioration with brainstem signs led to death in 15 patients. Electroencephalographic findings correlated well with the clinical course and eventual outcome. However, no correlation was found between seizure activity and the electroencephalogram. Autopsy findings in 14 cases included cerebral edema, tonsillar herniation, hypoxic neuronal degeneration, and fatty inflitration of the liver and kidneys."}
{"id": "PMID:1091787", "title": "A review of the research on general health status indexes.", "content": "During the mid-1960's, recognition of the spiraling cost of health care motivated Congress to enact several major pieces of legislation designed to underwrite efforts to improve the delivery of health services. The increased level of federal fiscal participation in the health service system has forced greater consciousness of the need for better accountability of the effectiveness and efficiency of the allocation of the dollar. Demands were articulated for precision tools which could evaluate the imput to output linkage between need and response; however, the tools were nascent and had limited applicability. Cost-effectiveness and cost-benefit analysis are two procedures that have since been applied with varying levels of success. A third tool, also developed as an outgrowth of the desire for a more accurate characterization of the planning concerns of an efficient/effective health service system, is the general health status index. The purpose of this article is to provide the reader with a comprehensive review of the literature on general health status indexes. Common objectives and constraints are presented, as well as a discussion of the expanding role of general health status indexes.", "contents": "A review of the research on general health status indexes. During the mid-1960's, recognition of the spiraling cost of health care motivated Congress to enact several major pieces of legislation designed to underwrite efforts to improve the delivery of health services. The increased level of federal fiscal participation in the health service system has forced greater consciousness of the need for better accountability of the effectiveness and efficiency of the allocation of the dollar. Demands were articulated for precision tools which could evaluate the imput to output linkage between need and response; however, the tools were nascent and had limited applicability. Cost-effectiveness and cost-benefit analysis are two procedures that have since been applied with varying levels of success. A third tool, also developed as an outgrowth of the desire for a more accurate characterization of the planning concerns of an efficient/effective health service system, is the general health status index. The purpose of this article is to provide the reader with a comprehensive review of the literature on general health status indexes. Common objectives and constraints are presented, as well as a discussion of the expanding role of general health status indexes."}
{"id": "PMID:1091788", "title": "An assessment of institutional review committees in human experimentation.", "content": "A study of the functioning of an institutional review committee, when combined with the Barber et al. survey data on 300 institutions, allows for a useful provisional assessment of the strengths and weaknesses of existing procedures for protecting human research subjects. The evidence shows that there is an important role for such committees; however, most committees are relatively permissive. While on committees' performance showed that such committees can have an impact on proposed research, the absence of effective monitoring procedures and lack of feedback from research subjects limits the impact of the review procedure on the actual conduct of research. The question is also raised whether the review procedure, because of its legitimizing functions, may have some regressive consequences for the protection of human subjects.", "contents": "An assessment of institutional review committees in human experimentation. A study of the functioning of an institutional review committee, when combined with the Barber et al. survey data on 300 institutions, allows for a useful provisional assessment of the strengths and weaknesses of existing procedures for protecting human research subjects. The evidence shows that there is an important role for such committees; however, most committees are relatively permissive. While on committees' performance showed that such committees can have an impact on proposed research, the absence of effective monitoring procedures and lack of feedback from research subjects limits the impact of the review procedure on the actual conduct of research. The question is also raised whether the review procedure, because of its legitimizing functions, may have some regressive consequences for the protection of human subjects."}
{"id": "PMID:1091792", "title": "Cell proliferation kinetics in tumors after treatments.", "content": "Analysis of data on cell reproductive death, cell proliferation kinetics, tumor volume responses and tumor eradication probability after single and fractionated doses of radiation given to experimental tumors, can yield several conclusions which may be relevant for the interpretation of clinical data: 1. considerable differences are observed among various cell types with respect to intrinsic radiosensitivity, but no specific differences are demonstrated for cells from tumors as compared to cells from normal tissue; 2. cell survival curves measured by in vitro cloning techniques for cells irradiated in an experimental rat rhabdomyosarcoma are consistent with the observed probability of tumor eradication, and 3. after a sufficiently large dose of radiation and after two weeks of fractionated treatments, important changes in cell proliferation kinetics have been observed with respect to cell cycle, cell production rate and cell loss rate, which may significantly influence the time of recurrence as well as the probability of attaining tumor eradication.", "contents": "Cell proliferation kinetics in tumors after treatments. Analysis of data on cell reproductive death, cell proliferation kinetics, tumor volume responses and tumor eradication probability after single and fractionated doses of radiation given to experimental tumors, can yield several conclusions which may be relevant for the interpretation of clinical data: 1. considerable differences are observed among various cell types with respect to intrinsic radiosensitivity, but no specific differences are demonstrated for cells from tumors as compared to cells from normal tissue; 2. cell survival curves measured by in vitro cloning techniques for cells irradiated in an experimental rat rhabdomyosarcoma are consistent with the observed probability of tumor eradication, and 3. after a sufficiently large dose of radiation and after two weeks of fractionated treatments, important changes in cell proliferation kinetics have been observed with respect to cell cycle, cell production rate and cell loss rate, which may significantly influence the time of recurrence as well as the probability of attaining tumor eradication."}
{"id": "PMID:1091793", "title": "Carcinogenesis of laryngeal carcinoma.", "content": "Experimental models have been developed for the induction of carcinoma of the larynx and for the study of its pathogenesis. The hamster has been the animal of choice. Polynuclear hydrocarbons, administered intratracheally, induce mostly squamous cell carcinomas. Diethylnitrosamine given systemically induces papillary tumors; some other N-nitroso-compounds given topically or systemically also induce laryngeal tumors, including carcinomas. Cigarette smoke inhalation induces preneoplastic and early neoplastic lesions of the larynx, including some invasive carcinomas. In vivo and in vitro methods have been developed for the morphological and biochemical study of target tissues in respiratory epithelial carcinogenesis to identify critical pathogenetic steps and their inhibition. These methods are now applicable to the in vitro study of human laryngeal epithelium and its susceptibility to cancer induction and prevention.", "contents": "Carcinogenesis of laryngeal carcinoma. Experimental models have been developed for the induction of carcinoma of the larynx and for the study of its pathogenesis. The hamster has been the animal of choice. Polynuclear hydrocarbons, administered intratracheally, induce mostly squamous cell carcinomas. Diethylnitrosamine given systemically induces papillary tumors; some other N-nitroso-compounds given topically or systemically also induce laryngeal tumors, including carcinomas. Cigarette smoke inhalation induces preneoplastic and early neoplastic lesions of the larynx, including some invasive carcinomas. In vivo and in vitro methods have been developed for the morphological and biochemical study of target tissues in respiratory epithelial carcinogenesis to identify critical pathogenetic steps and their inhibition. These methods are now applicable to the in vitro study of human laryngeal epithelium and its susceptibility to cancer induction and prevention."}
{"id": "PMID:1091795", "title": "Indirect laryngoscopy.", "content": "Indirect laryngoscopy is one of the procedural methods used for achieving a histopathologic diagnosis of cancer of the larynx. A chronologic historical summary of the development of mirror laryngoscopy is presented. The details of the procedure employed for obtaining a biopsy with the aid of topical anesthesia is discussed.", "contents": "Indirect laryngoscopy. Indirect laryngoscopy is one of the procedural methods used for achieving a histopathologic diagnosis of cancer of the larynx. A chronologic historical summary of the development of mirror laryngoscopy is presented. The details of the procedure employed for obtaining a biopsy with the aid of topical anesthesia is discussed."}
{"id": "PMID:1091797", "title": "Post-laryngectomy vocal rehabilitation using expired air and an external fistula method.", "content": "The advantages and problems of fistula speech in post-laryngectomy vocal rehabilitation are discussed. A pilot study of laryngectomees has shown the practical advantages and low risk of a primary one-stage external vocal fistula construction at the time of laryngectomy, allowing very early fistula speech. These results indicate the need for an intensive program to develop a definitive surgical procedure and prosthesis, with a view to applying the method to all types of laryngectomees.", "contents": "Post-laryngectomy vocal rehabilitation using expired air and an external fistula method. The advantages and problems of fistula speech in post-laryngectomy vocal rehabilitation are discussed. A pilot study of laryngectomees has shown the practical advantages and low risk of a primary one-stage external vocal fistula construction at the time of laryngectomy, allowing very early fistula speech. These results indicate the need for an intensive program to develop a definitive surgical procedure and prosthesis, with a view to applying the method to all types of laryngectomees."}
{"id": "PMID:1091798", "title": "Medial conchal excision in otoplasty.", "content": "The most common cartilaginous deformities in protruding ears include an inadequate antihelical fold and an enlarged concha. We have employed medial conchal excision to correct deep conchas in 25 patients, all of whom were pleased with the postoperative result. Obvious wrinkling of the anterior skin occurred in four patients, and several patients displayed slight distortion of the helical crus. Medial conchal excision does not impede management of the antihelical fold with mattress sutures or by other means. We consider the risk of minor alterations of the concha justified if even slight distortion of the more conspicuous lateral auricle can be avoided.", "contents": "Medial conchal excision in otoplasty. The most common cartilaginous deformities in protruding ears include an inadequate antihelical fold and an enlarged concha. We have employed medial conchal excision to correct deep conchas in 25 patients, all of whom were pleased with the postoperative result. Obvious wrinkling of the anterior skin occurred in four patients, and several patients displayed slight distortion of the helical crus. Medial conchal excision does not impede management of the antihelical fold with mattress sutures or by other means. We consider the risk of minor alterations of the concha justified if even slight distortion of the more conspicuous lateral auricle can be avoided."}
{"id": "PMID:1091822", "title": "Treatment of hypertension with an antihypertensive agent possessing vasodilator activity.", "content": "Administration of the vasodilator agent, prazosin, in dosages from 3-0 to 7-5 mg per day, significantly reduced diastolic and systolic blood pressure in a group of 14 patients with mild or moderate hypertension. Nine of these patients had a lowering of diastolic blood pressure which was at least 10 mm Hg greater than that caused by placebo. Side effects, chiefly dizziness or lassitude, occurred in over half the patients, but were mild and of short duration and did not necessitate suspension of treatment. The antihypertensive effect of prazosin was similar to that produced by methyldopa or propranolol given in comparable doses. If prazosin and propranolol were given concurrently, blood pressure fell to a level which was significantly lower than that associated with the use of either of these agents alone.", "contents": "Treatment of hypertension with an antihypertensive agent possessing vasodilator activity. Administration of the vasodilator agent, prazosin, in dosages from 3-0 to 7-5 mg per day, significantly reduced diastolic and systolic blood pressure in a group of 14 patients with mild or moderate hypertension. Nine of these patients had a lowering of diastolic blood pressure which was at least 10 mm Hg greater than that caused by placebo. Side effects, chiefly dizziness or lassitude, occurred in over half the patients, but were mild and of short duration and did not necessitate suspension of treatment. The antihypertensive effect of prazosin was similar to that produced by methyldopa or propranolol given in comparable doses. If prazosin and propranolol were given concurrently, blood pressure fell to a level which was significantly lower than that associated with the use of either of these agents alone."}
{"id": "PMID:1091831", "title": "[Season and psychiatric disease (author's transl)].", "content": "Clinical experience shows that certain psychiatric patients have to be hospitalized more frequently during certain periods of the year. We compared the data of the hospitalisation of 17268 psychiatric patients, hospitalized over a period of 13 years (1956-1969) with the time of the year. Schizophrenics are hospitalized mostly during the warm period, least during the cold period, regardless of their sex. The same can be said about the diagnostic subgroups (paranoics, catatonics, hebephrenics, schizophrenia simplex) and the periodic depressions and depressions of involution. Reactive and neurotic depressions and depressions of exhaustion, neurotics (crisis during puberty, neuroses of character, obsessional and anxiety neuroses), arteriosclerotics and psychopaths do not show an even pattern of distribution. Male alcoholics, too, do not show a maximum of admissions. Female alcoholics are admitted more during the warm periods, less often during the cold periods. Drug addicts, too, show a minimum in winter, an even level over the rest of the time of the year. It is suggested that the patterns of admissions for certain psychiatric patients are to be explained by the different sensibility of meteorological factors.", "contents": "[Season and psychiatric disease (author's transl)]. Clinical experience shows that certain psychiatric patients have to be hospitalized more frequently during certain periods of the year. We compared the data of the hospitalisation of 17268 psychiatric patients, hospitalized over a period of 13 years (1956-1969) with the time of the year. Schizophrenics are hospitalized mostly during the warm period, least during the cold period, regardless of their sex. The same can be said about the diagnostic subgroups (paranoics, catatonics, hebephrenics, schizophrenia simplex) and the periodic depressions and depressions of involution. Reactive and neurotic depressions and depressions of exhaustion, neurotics (crisis during puberty, neuroses of character, obsessional and anxiety neuroses), arteriosclerotics and psychopaths do not show an even pattern of distribution. Male alcoholics, too, do not show a maximum of admissions. Female alcoholics are admitted more during the warm periods, less often during the cold periods. Drug addicts, too, show a minimum in winter, an even level over the rest of the time of the year. It is suggested that the patterns of admissions for certain psychiatric patients are to be explained by the different sensibility of meteorological factors."}
{"id": "PMID:1091838", "title": "[Balkan-nephropathy, a particular form of interstitial nephritis (author's transl)].", "content": "Because of epidemiological, clinical, pathomorphological, and etiological criteria the Balkan-nephropathy is suggested to be a particular form of chronic interstitial nephritis with super-imposed pyelonephritis in about 30 p.c. of the patients. A basic scheme illustrates the origin and the development of the endemia as well as etiology and clinical course of the disease. Another scheme shows pathogenesis and pathomorphogenesis of the nephritis. This analysis about the characteristics of the endemic Balkan-nephropathy allows for the clarification of the triad: endemic occurrance, familial susceptibility, and mosaik like morbidity. The following important aspects of the disease are given: rarely occuring hypertension, facultative leukuria, and bacteriuria, smooth nephrocirrhosis. Prophylactic and therapeutic prospects are given.", "contents": "[Balkan-nephropathy, a particular form of interstitial nephritis (author's transl)]. Because of epidemiological, clinical, pathomorphological, and etiological criteria the Balkan-nephropathy is suggested to be a particular form of chronic interstitial nephritis with super-imposed pyelonephritis in about 30 p.c. of the patients. A basic scheme illustrates the origin and the development of the endemia as well as etiology and clinical course of the disease. Another scheme shows pathogenesis and pathomorphogenesis of the nephritis. This analysis about the characteristics of the endemic Balkan-nephropathy allows for the clarification of the triad: endemic occurrance, familial susceptibility, and mosaik like morbidity. The following important aspects of the disease are given: rarely occuring hypertension, facultative leukuria, and bacteriuria, smooth nephrocirrhosis. Prophylactic and therapeutic prospects are given."}
{"id": "PMID:1091849", "title": "The protein topography of the E. coli 30S ribosomal subunit: a preliminary model E. coli/30S subunit/ribosome/spatial arrangement of proteins.", "content": "A three dimensional model is presented which shows the apatial arrangement of 20 of the 21 proteins of the 30S ribosomal subunit of Escherichia coli. The model fulfills several purposes: (a) It summarizes currently available structural and functional data on ribosomal proteins; (b) It suggests an interesting correlation between stoichiometry and function. Functional proteins are both clustered and fractional; (c) It can be evaluated in relationships or point out critical experiments by which it can be tested.", "contents": "The protein topography of the E. coli 30S ribosomal subunit: a preliminary model E. coli/30S subunit/ribosome/spatial arrangement of proteins. A three dimensional model is presented which shows the apatial arrangement of 20 of the 21 proteins of the 30S ribosomal subunit of Escherichia coli. The model fulfills several purposes: (a) It summarizes currently available structural and functional data on ribosomal proteins; (b) It suggests an interesting correlation between stoichiometry and function. Functional proteins are both clustered and fractional; (c) It can be evaluated in relationships or point out critical experiments by which it can be tested."}
{"id": "PMID:1091851", "title": "Isocitrate dehydrogenases and oxoglutarate dehydrogenase activities of baker's yeast grown in a variety of hypoxic conditions.", "content": "The activities of isocitrate dehydrogenase (NAD), isocitrate dehydrogenase (NADP) and oxoglutarate dehydrogenase have been investigated in Saccharomyces cerevisiae grown in a variety of aerobic and hypoxic conditions, the latter including oxygen deprivation, high glucose concentration, addition of inhibitors of mitochondrial protein synthesis, respiratory inhibition by azide, and impaired respiration mutants. All hypoxic conditions led to a marked decrease of oxoglutarate dehydrogenase and significant decreases of the two isocitrate dehydrogenases. According to its kinetic properties, the NAD-isocitrate dehydrogenase will not be operative in hypoxia \"in vivo\". From these and other related facts it is concluded that hypoxic conditions in yeast generally lead to a splitting of the tricarboxylic acid cycle and that glutamate synthesis in these conditions takes place through the coupling of the NADP-linked isocitrate and glutamate dehydrogenases.", "contents": "Isocitrate dehydrogenases and oxoglutarate dehydrogenase activities of baker's yeast grown in a variety of hypoxic conditions. The activities of isocitrate dehydrogenase (NAD), isocitrate dehydrogenase (NADP) and oxoglutarate dehydrogenase have been investigated in Saccharomyces cerevisiae grown in a variety of aerobic and hypoxic conditions, the latter including oxygen deprivation, high glucose concentration, addition of inhibitors of mitochondrial protein synthesis, respiratory inhibition by azide, and impaired respiration mutants. All hypoxic conditions led to a marked decrease of oxoglutarate dehydrogenase and significant decreases of the two isocitrate dehydrogenases. According to its kinetic properties, the NAD-isocitrate dehydrogenase will not be operative in hypoxia \"in vivo\". From these and other related facts it is concluded that hypoxic conditions in yeast generally lead to a splitting of the tricarboxylic acid cycle and that glutamate synthesis in these conditions takes place through the coupling of the NADP-linked isocitrate and glutamate dehydrogenases."}
{"id": "PMID:1091850", "title": "The adrenal medulla: a model for studies of hormonal and neuronal storage and release mechanisms.", "content": "We have observed that phospholipids and protein of the catecholamine (CA) storage granules, i.e. the chromaffin granules, interact in an in vitro system to form liposomal particles, which in many respects resemble the intact matrix of the bovine chromaffin granule. A model has been suggested which consists of an aqueous phase, containing the acidic chromogranins and intact dopamine-beta-hydroxylase (DBH) ATP and CA, embedded in a liquid crystal of the matrix phospholipids. Ca2+ may play a significant role in the sequence of functional transitions of such an organelle, not only in the accumulation of Ca2+, as during the secretory phase of the intact cell, but also as the agent inducing a separation of the outer membrane bilayer from the matrix phase to be released, as during exocytosis. Furthermore, a liposome model of the matrix may also tentatively explain the occurrence of intact matrices in the interstitium of stimulated glands. Recent evidence for the identity between chromogranin A and DBH subunits have been summarized and a possible role for the inactive subunits in the ionic binding of ATP and CA in the aqueous phase of the matrix is discussed. A role of Ca2+ and cyclic AMP in the mediation of beta-adrenergic modulation is postulated on the basis of our recent work on acetylcholine-induced release of CA from perfused and stimulated bovine adrenals. We conclude that such a beta-adrenergic modulation is secondary to that of the cholinergic response. Hence, this activation is able to enhance the output induced by mild cholinergic stimulation although insufficient to evoke a CA release by itself.", "contents": "The adrenal medulla: a model for studies of hormonal and neuronal storage and release mechanisms. We have observed that phospholipids and protein of the catecholamine (CA) storage granules, i.e. the chromaffin granules, interact in an in vitro system to form liposomal particles, which in many respects resemble the intact matrix of the bovine chromaffin granule. A model has been suggested which consists of an aqueous phase, containing the acidic chromogranins and intact dopamine-beta-hydroxylase (DBH) ATP and CA, embedded in a liquid crystal of the matrix phospholipids. Ca2+ may play a significant role in the sequence of functional transitions of such an organelle, not only in the accumulation of Ca2+, as during the secretory phase of the intact cell, but also as the agent inducing a separation of the outer membrane bilayer from the matrix phase to be released, as during exocytosis. Furthermore, a liposome model of the matrix may also tentatively explain the occurrence of intact matrices in the interstitium of stimulated glands. Recent evidence for the identity between chromogranin A and DBH subunits have been summarized and a possible role for the inactive subunits in the ionic binding of ATP and CA in the aqueous phase of the matrix is discussed. A role of Ca2+ and cyclic AMP in the mediation of beta-adrenergic modulation is postulated on the basis of our recent work on acetylcholine-induced release of CA from perfused and stimulated bovine adrenals. We conclude that such a beta-adrenergic modulation is secondary to that of the cholinergic response. Hence, this activation is able to enhance the output induced by mild cholinergic stimulation although insufficient to evoke a CA release by itself."}
{"id": "PMID:1091854", "title": "Effects of p-fluorophenylalanine on the induction of mutations in bacteriophage T4. I. 5-Bromouracil mutagenesis.", "content": "The amino acid analogue rho-fluorophenylalanine (PFPA) was found to have no mutagenic activity in the gamma system of bacteriophage T4. However, under standard conditions for 5-bromouracil (5-BU) mutagenesis, PFPA depressed the induced frequencies for both forward and reverse mutations. When the folate antagonist sulphanilamide (SU) was omitted from the mutangenic treatment medium or when it was replaced by Trimethoprim (TM), another folate antagonist, this depressive effect was abolished. It was proposed that PFPA alleviated the inhibitory action of SU.", "contents": "Effects of p-fluorophenylalanine on the induction of mutations in bacteriophage T4. I. 5-Bromouracil mutagenesis. The amino acid analogue rho-fluorophenylalanine (PFPA) was found to have no mutagenic activity in the gamma system of bacteriophage T4. However, under standard conditions for 5-bromouracil (5-BU) mutagenesis, PFPA depressed the induced frequencies for both forward and reverse mutations. When the folate antagonist sulphanilamide (SU) was omitted from the mutangenic treatment medium or when it was replaced by Trimethoprim (TM), another folate antagonist, this depressive effect was abolished. It was proposed that PFPA alleviated the inhibitory action of SU."}
{"id": "PMID:1091855", "title": "Enterotoxigenic Escherichia-coli-associated diarrheal disease in Apache children.", "content": "A search for intestinal enterotoxigenic Escherichia coli was made in 59 Apache children hospitalized with 64 episodes of acute diarrhea. Esch. coli isolates from acute-phase and convalescent-phase specimens of small-bowel fluid and stool were tested in three currently recognized models: the adult-rabbit ileal loop; infant rabbit; and the adrenal-cell assay. Enterotoxigenic strains were isolated from 10 children during acute diarrheal episodes (16 per cent); none were isolated from convalescent-phase specimens. None of 64 \"enteropathogenic\" serotypes of Esch. coli from 43 children with diarrhea, however, caused fluid production in the ileal-loop model. These results suggest that enterotoxigenic Esch. coli may be the cause of considerable diarrhea in this population and that the term \"enteropathogenic\" as applied to serotypes of Esch. coli needs to be redefined.", "contents": "Enterotoxigenic Escherichia-coli-associated diarrheal disease in Apache children. A search for intestinal enterotoxigenic Escherichia coli was made in 59 Apache children hospitalized with 64 episodes of acute diarrhea. Esch. coli isolates from acute-phase and convalescent-phase specimens of small-bowel fluid and stool were tested in three currently recognized models: the adult-rabbit ileal loop; infant rabbit; and the adrenal-cell assay. Enterotoxigenic strains were isolated from 10 children during acute diarrheal episodes (16 per cent); none were isolated from convalescent-phase specimens. None of 64 \"enteropathogenic\" serotypes of Esch. coli from 43 children with diarrhea, however, caused fluid production in the ileal-loop model. These results suggest that enterotoxigenic Esch. coli may be the cause of considerable diarrhea in this population and that the term \"enteropathogenic\" as applied to serotypes of Esch. coli needs to be redefined."}
{"id": "PMID:1091856", "title": "Management of heparin therapy: Controlled prospective trial.", "content": "Among 100 consecutive patients receiving heparin in therapeutic dosage, major bleeding occurred in 21, and minor bleeding in 16. Two patients died from bleeding, and two had recurrent pulmonary embolism. Major bleeding occurred in 21% when therapy was regulated with whole-blood clotting time and in 20% when heparin was given without clotting tests. In a subsequent prospective trial patients received heparin by intermittent intravenous injection with or without laboratory control according to the partial thromboplastin time or continuously by intravenous infusion. Recurrent thromboembolism occurred once in each group. Major bleeding was seven times more frequent with intermittent injection than with continuous infusion. Control with the partial thromboplastin time did not prevent major bleeding in patients receiving intermittent injections. With continuous infusion, one-fourth less heparin was required than with intermittent injections. Administration of heparin by continuous infusion appears safer than intermittent injection with or without laboratory control and is no less effective for prevention of thromboembolism.", "contents": "Management of heparin therapy: Controlled prospective trial. Among 100 consecutive patients receiving heparin in therapeutic dosage, major bleeding occurred in 21, and minor bleeding in 16. Two patients died from bleeding, and two had recurrent pulmonary embolism. Major bleeding occurred in 21% when therapy was regulated with whole-blood clotting time and in 20% when heparin was given without clotting tests. In a subsequent prospective trial patients received heparin by intermittent intravenous injection with or without laboratory control according to the partial thromboplastin time or continuously by intravenous infusion. Recurrent thromboembolism occurred once in each group. Major bleeding was seven times more frequent with intermittent injection than with continuous infusion. Control with the partial thromboplastin time did not prevent major bleeding in patients receiving intermittent injections. With continuous infusion, one-fourth less heparin was required than with intermittent injections. Administration of heparin by continuous infusion appears safer than intermittent injection with or without laboratory control and is no less effective for prevention of thromboembolism."}
{"id": "PMID:1091864", "title": "Trilobite eyes and the optics of Des Cartes and Huygens.", "content": "The thick lenses in the aggregate eyes of a group of trilobites were double structures designed to eliminate spherical aberration. The shape of the optically correcting interface is in accord with constructions by Des Cartes and Huygens and is dictated by a fundamental law of physics. Trilobites may have evolved such sophisticated eye-lenses to maximise optic neurone response in a dimly lit environment.", "contents": "Trilobite eyes and the optics of Des Cartes and Huygens. The thick lenses in the aggregate eyes of a group of trilobites were double structures designed to eliminate spherical aberration. The shape of the optically correcting interface is in accord with constructions by Des Cartes and Huygens and is dictated by a fundamental law of physics. Trilobites may have evolved such sophisticated eye-lenses to maximise optic neurone response in a dimly lit environment."}
{"id": "PMID:1091866", "title": "Nasal infection of Alcaligenes bronchisepticus (Bordetella bronchiseptica) and lesions in newborn rabbits.", "content": "The mode of experimental infection with Alcaligenes bronchisepticus (Bordetella bronchiseptica) of pig and rabbit origin, and lesions in the respiratory organs were examined in three groups, A, B and C, of newborn rabbits. Groups A and C were free from the organism and agglutinating antibody and inoculated with the organisms of pig and rabbit origin, respectively. Group B had maternal antibody and was inoculated with the organism of pig origin. Establishment and persistence of infection with the organism were certified in the nasal cavities, trachea, or lungs of groups A and C 3 days after inoculation or later. Late establishment of infection occurred in trachea and lungs of group B. Agglutinating antibody was detected in groups A and B mostly 14 days after inoculation or later. Ventral turbinate atrophy occurred in groups A and B mostly 10 days after inoculation or later. Histologically, it was hypo-osteogenesis caused by degeneration of osteoblasts and proliferation of fibroblast-like cells in the osseous tissue. Catarrhal inflammation in the nasal and tracheal mucous membranes, and bronchopneumonia with peribronchiolitis developed commonly in all the groups. The fluorescent antibody technique revealed antigen of the organism of pig origin on the nasal mucosa, mostly of the dorsal and ventral meatus, and on the tracheal and bronchiolar mucosa in groups A and B.", "contents": "Nasal infection of Alcaligenes bronchisepticus (Bordetella bronchiseptica) and lesions in newborn rabbits. The mode of experimental infection with Alcaligenes bronchisepticus (Bordetella bronchiseptica) of pig and rabbit origin, and lesions in the respiratory organs were examined in three groups, A, B and C, of newborn rabbits. Groups A and C were free from the organism and agglutinating antibody and inoculated with the organisms of pig and rabbit origin, respectively. Group B had maternal antibody and was inoculated with the organism of pig origin. Establishment and persistence of infection with the organism were certified in the nasal cavities, trachea, or lungs of groups A and C 3 days after inoculation or later. Late establishment of infection occurred in trachea and lungs of group B. Agglutinating antibody was detected in groups A and B mostly 14 days after inoculation or later. Ventral turbinate atrophy occurred in groups A and B mostly 10 days after inoculation or later. Histologically, it was hypo-osteogenesis caused by degeneration of osteoblasts and proliferation of fibroblast-like cells in the osseous tissue. Catarrhal inflammation in the nasal and tracheal mucous membranes, and bronchopneumonia with peribronchiolitis developed commonly in all the groups. The fluorescent antibody technique revealed antigen of the organism of pig origin on the nasal mucosa, mostly of the dorsal and ventral meatus, and on the tracheal and bronchiolar mucosa in groups A and B."}
{"id": "PMID:1091870", "title": "Pharmacologic approach to the maintenance of urate homeostasis.", "content": "A reduction in the serum urate concentration in hyperuricemic subjects can be achieved by increasing the renal excretion of uric acid, by inhibiting its synthesis, or by a combination of both modalities. The three most commonly used hypouricemic drugs are probenecid, sulfinpyrazone and allopurinol. Each drug has been considered in detail with regard to its mechanism of action, metabolism, diverse metabolic effects, side effects and interaction with other drugs. In addition, guidelines for the use of these drugs in hyperuricemic subjects are proposed.", "contents": "Pharmacologic approach to the maintenance of urate homeostasis. A reduction in the serum urate concentration in hyperuricemic subjects can be achieved by increasing the renal excretion of uric acid, by inhibiting its synthesis, or by a combination of both modalities. The three most commonly used hypouricemic drugs are probenecid, sulfinpyrazone and allopurinol. Each drug has been considered in detail with regard to its mechanism of action, metabolism, diverse metabolic effects, side effects and interaction with other drugs. In addition, guidelines for the use of these drugs in hyperuricemic subjects are proposed."}
{"id": "PMID:1091873", "title": "Stimulatory effect of prostaglandin E2 on LH release in the rat: evidence for hypothalamic site of action.", "content": "The intravenous administration of prostaglandin E2 (PGE2; 100 mug/rat) to immature male and female rats increased the serum level of lutenizing hormone (LH). This effect was prevented by the prior administration of an antiserum to the hypothalamic gonadotropin-releasing hormone. LH release from rat pituitaries in vitro was not stimulated by PGE2 (10 mug/ml). It is inferred that the stimulatory effect of PGE2, on LH release is not a direct one on the pituitary gland, but is exerted at the level of the hypothalamus.", "contents": "Stimulatory effect of prostaglandin E2 on LH release in the rat: evidence for hypothalamic site of action. The intravenous administration of prostaglandin E2 (PGE2; 100 mug/rat) to immature male and female rats increased the serum level of lutenizing hormone (LH). This effect was prevented by the prior administration of an antiserum to the hypothalamic gonadotropin-releasing hormone. LH release from rat pituitaries in vitro was not stimulated by PGE2 (10 mug/ml). It is inferred that the stimulatory effect of PGE2, on LH release is not a direct one on the pituitary gland, but is exerted at the level of the hypothalamus."}
{"id": "PMID:1091874", "title": "Variation of the pituitary response to LH-releasing hormone (LH-RH) during a 24-hour period in male, diestrous female and androgenized female rats.", "content": "The pituitary response to LH-RH was studied at 8 a.m., 4 p.m. and 12 p.m. in normal male rats, female rats on day 1 of diestrus and female rats injected with testosterone propionate on the 2nd day of life. Androgenized rats responded to LH-RH with a greater release of LH than did female diestrous and male rats. In the 3 groups the lowest response to LH-RH was observed at 12 p.m., and the highest at 8 a.m., with the exception of androgenized rats which responded with a similar magnitude of LH release at 8 a.m. and 4 p.m. The release of FSH after the injection of LH-RH was studied only at 4 p.m. and only normal male rats responded with a significant FSH release.", "contents": "Variation of the pituitary response to LH-releasing hormone (LH-RH) during a 24-hour period in male, diestrous female and androgenized female rats. The pituitary response to LH-RH was studied at 8 a.m., 4 p.m. and 12 p.m. in normal male rats, female rats on day 1 of diestrus and female rats injected with testosterone propionate on the 2nd day of life. Androgenized rats responded to LH-RH with a greater release of LH than did female diestrous and male rats. In the 3 groups the lowest response to LH-RH was observed at 12 p.m., and the highest at 8 a.m., with the exception of androgenized rats which responded with a similar magnitude of LH release at 8 a.m. and 4 p.m. The release of FSH after the injection of LH-RH was studied only at 4 p.m. and only normal male rats responded with a significant FSH release."}
{"id": "PMID:1091895", "title": "Treatment of adenocarcinoma of the endometrium.", "content": "The evidence concerning those factors related to prognosis in endometrial carcinoma has been reviewed and age, histologic differentiation of the tumor, depth of myometrial invasion and extent of disease have been found to be definitely correlated with survival. The development of different treatment methods has been traced and various modern treatment techniques have been discussed. On the basis of these prognostic indicators and reported experience with alternative methods of therapy, a plan of management for patients with endometrial carcinoma has been proposed. Although we believe this is a logical method with proven effectiveness, we wish to emphasize the importance of individualized therapy based on an understanding of the clinical-pathological findings.", "contents": "Treatment of adenocarcinoma of the endometrium. The evidence concerning those factors related to prognosis in endometrial carcinoma has been reviewed and age, histologic differentiation of the tumor, depth of myometrial invasion and extent of disease have been found to be definitely correlated with survival. The development of different treatment methods has been traced and various modern treatment techniques have been discussed. On the basis of these prognostic indicators and reported experience with alternative methods of therapy, a plan of management for patients with endometrial carcinoma has been proposed. Although we believe this is a logical method with proven effectiveness, we wish to emphasize the importance of individualized therapy based on an understanding of the clinical-pathological findings."}
{"id": "PMID:1091896", "title": "Gynecologic malignancies in immunosuppressed organ homograft recipients.", "content": "Immunosuppressed organ homograft recipients have a 5 to 6% incidence of de novo malignancies at some time after transplantation. Gynecologic cancers were encountered in 21 of 224 patients (9%) with these tumors. The predominant lesion was carcinoma of the cervix (18 cases), of which 16 were intraepithelial and 2 were invasive. Gynecologic malignancies have also been encountered in non-transplant patients who were treated with immunosuppressive agents or cancer chemotherapy. All such individuals require gynecologic examination before commencement of treatment and at regular intervals thereafter so that malignancies may be diagnosed at an early stage and treated effectively. Most neoplasms respond well to conventional cancer therapy, but high-grade malignancies may necessitate reduction or cessation of immunosuppressive therapy as well.", "contents": "Gynecologic malignancies in immunosuppressed organ homograft recipients. Immunosuppressed organ homograft recipients have a 5 to 6% incidence of de novo malignancies at some time after transplantation. Gynecologic cancers were encountered in 21 of 224 patients (9%) with these tumors. The predominant lesion was carcinoma of the cervix (18 cases), of which 16 were intraepithelial and 2 were invasive. Gynecologic malignancies have also been encountered in non-transplant patients who were treated with immunosuppressive agents or cancer chemotherapy. All such individuals require gynecologic examination before commencement of treatment and at regular intervals thereafter so that malignancies may be diagnosed at an early stage and treated effectively. Most neoplasms respond well to conventional cancer therapy, but high-grade malignancies may necessitate reduction or cessation of immunosuppressive therapy as well."}
{"id": "PMID:1091897", "title": "Relation of lichen sclerosus et atrophicus of the vulva to development of carcinoma.", "content": "One hundred and seven patients with lichen sclerosus et atrophicus (LS&A) of the vulva were studies to determine the malignant potential of the LS&A. Five patients had coexisting invasive carcinoma of the vulva or perineum with the LS&A, and 1 patient had coexisting intraepithelial vulvar carcinoma on the clitoris. None of these, however, was known to have LS&A prior to the biopsy for carcinoma of the vulva. The high association of carcinoma and LS*A is probably a result of selection of 2 unusual lesions sent for consultation and evaluation. Squamous hyperplasia in the vulva occurred in association with LS&A in 37 (35%) patients, but only 6 patients had areas of dysplasia coexisting with LS&A. These areas of dysplasia, like the 5 invasive carcinomas, occurred in an area of the vulva where the LS&A was minimal or absent. Follow-up data were obtained on 92 patients with LS&A. Only 1 developed carcinoma of the vulva, which occurred 12 years after identification of the LS&A. When carcinoma arises in the vulva in a patient with vulvar LS&A, it tends to arise in areas of minomal LS&A or isolated areas of relatively normal vulvar skin. This study did not provide evidence of carcinoma arising from LS&A. Five of the 92 patients developed 6 malignant neoplasms in other sites, including carcinoma of the endometrium (3 patients), lung (1 patient), and simultaneous carcinomas of the colon and cervix (1 patient).", "contents": "Relation of lichen sclerosus et atrophicus of the vulva to development of carcinoma. One hundred and seven patients with lichen sclerosus et atrophicus (LS&A) of the vulva were studies to determine the malignant potential of the LS&A. Five patients had coexisting invasive carcinoma of the vulva or perineum with the LS&A, and 1 patient had coexisting intraepithelial vulvar carcinoma on the clitoris. None of these, however, was known to have LS&A prior to the biopsy for carcinoma of the vulva. The high association of carcinoma and LS*A is probably a result of selection of 2 unusual lesions sent for consultation and evaluation. Squamous hyperplasia in the vulva occurred in association with LS&A in 37 (35%) patients, but only 6 patients had areas of dysplasia coexisting with LS&A. These areas of dysplasia, like the 5 invasive carcinomas, occurred in an area of the vulva where the LS&A was minimal or absent. Follow-up data were obtained on 92 patients with LS&A. Only 1 developed carcinoma of the vulva, which occurred 12 years after identification of the LS&A. When carcinoma arises in the vulva in a patient with vulvar LS&A, it tends to arise in areas of minomal LS&A or isolated areas of relatively normal vulvar skin. This study did not provide evidence of carcinoma arising from LS&A. Five of the 92 patients developed 6 malignant neoplasms in other sites, including carcinoma of the endometrium (3 patients), lung (1 patient), and simultaneous carcinomas of the colon and cervix (1 patient)."}
{"id": "PMID:1091899", "title": "Responsibility of the obstetrician to the fetus. II. Influence of prepregnancy weight and pregnancy weight gain on birthweight.", "content": "The diet of pregnant women has been restricted in various ways since earliest history. Dietary restriction may have severely deleterious effects on the fetus. Our study revealed a positive correlation of prepregnancy weight and pregnancy weight gain to term infant birthweight. Their influences are independent and additive. It is of the utmost importance to the fetus and the future of the human race that the diet of pregnant women contain the caloric value and essential nutrients recommended by the National Research Council.", "contents": "Responsibility of the obstetrician to the fetus. II. Influence of prepregnancy weight and pregnancy weight gain on birthweight. The diet of pregnant women has been restricted in various ways since earliest history. Dietary restriction may have severely deleterious effects on the fetus. Our study revealed a positive correlation of prepregnancy weight and pregnancy weight gain to term infant birthweight. Their influences are independent and additive. It is of the utmost importance to the fetus and the future of the human race that the diet of pregnant women contain the caloric value and essential nutrients recommended by the National Research Council."}
{"id": "PMID:1091900", "title": "Enchancement of deficient pituitary response to luteinizing hormone releasing hormone in patients with primary amenorrhea.", "content": "Although the absence of pituitary response to the luteinizing hormone releasing hormone (LHRH) test has been considered proof of a lesion primarily localized at the pituitary level, the possibility exists that an absent pituitary response may represent only the effect of a chronic deficiency of hypothalamic secretion. To verify this hypothesis, 4 patients with primary amenorrhea, hypogonadotropic hypogonadism, and deficient or absent responses to a 25 mug LHRH rapid IV test were treated with 400 mug LHRH infused in 7 hours during each of 3 successive days. The finding that patients with deficient LH responses to a rapid LHRH test became normally responsive to a second equivalent test after a slow and prolonged treatment with the decapeptide suggests that, in these patients, besides a lesion at the pituitary level, a primary defect at the hypothalamic or higher centers may also be suspected.", "contents": "Enchancement of deficient pituitary response to luteinizing hormone releasing hormone in patients with primary amenorrhea. Although the absence of pituitary response to the luteinizing hormone releasing hormone (LHRH) test has been considered proof of a lesion primarily localized at the pituitary level, the possibility exists that an absent pituitary response may represent only the effect of a chronic deficiency of hypothalamic secretion. To verify this hypothesis, 4 patients with primary amenorrhea, hypogonadotropic hypogonadism, and deficient or absent responses to a 25 mug LHRH rapid IV test were treated with 400 mug LHRH infused in 7 hours during each of 3 successive days. The finding that patients with deficient LH responses to a rapid LHRH test became normally responsive to a second equivalent test after a slow and prolonged treatment with the decapeptide suggests that, in these patients, besides a lesion at the pituitary level, a primary defect at the hypothalamic or higher centers may also be suspected."}
{"id": "PMID:1091904", "title": "[The graphic test \"the enchanted family\" and its application in educational counselling and paediatric clinics (author's transl)].", "content": "Projectional tests using pictures, drawings, story-telling and puppets induce children to exhibit their conflicts within the family. Among such drawing tests, \"The Enchanted Family\" asks the child to put the members of a family under the spell of a magician, without any limits being set on the child's imagination in the choice of objects to be drawn. The psychological content is then underlined by the child telling a story about the casting of the spell. Four thousand children and juveniles, of which 1562 had behavioural disturbances or were neurotically or psychosomatically ill, were examined with this test and 1225 of these were statistically evaluated for the formal aspects of their drawings. A group of healthy school children were evaluated as controls. In addition to the formal results, the depth-psychological results of the tests offered valuable pointers to the individual conflict situations of the test subjects, in particular in relation to the relationships within the family, the projections and identifications in \"key situations\" of the neurotic family behaviour patterns. This was demonstrated in individual case studies. The symbolism in the choice of animals receives specific interpretation. The use of this test in educational advisory practice and psychotherapy is recommended.", "contents": "[The graphic test \"the enchanted family\" and its application in educational counselling and paediatric clinics (author's transl)]. Projectional tests using pictures, drawings, story-telling and puppets induce children to exhibit their conflicts within the family. Among such drawing tests, \"The Enchanted Family\" asks the child to put the members of a family under the spell of a magician, without any limits being set on the child's imagination in the choice of objects to be drawn. The psychological content is then underlined by the child telling a story about the casting of the spell. Four thousand children and juveniles, of which 1562 had behavioural disturbances or were neurotically or psychosomatically ill, were examined with this test and 1225 of these were statistically evaluated for the formal aspects of their drawings. A group of healthy school children were evaluated as controls. In addition to the formal results, the depth-psychological results of the tests offered valuable pointers to the individual conflict situations of the test subjects, in particular in relation to the relationships within the family, the projections and identifications in \"key situations\" of the neurotic family behaviour patterns. This was demonstrated in individual case studies. The symbolism in the choice of animals receives specific interpretation. The use of this test in educational advisory practice and psychotherapy is recommended."}
{"id": "PMID:1091910", "title": "Skin and soft tissue injuries of the hand in children.", "content": "Three pediatric hand injury subtypes and their management have been presented. Finger tip injuries, while often minor and self-healing in children, may require surgical repair if the nailbed is disrupted or if there has been significant skin or pad loss from the tip. The decision between local repair and skin graft or pedicle reconstruction depends on the nature and extent of the injury. Multiple digit injuries should be carefully evaluated and manged with a view toward retention of digit length and restoration of function. Immediate closure of all wounds, while desirable, is not necessary. Minor hand burns are quite easily managed but burn injuries more severe in extent and/or depth must be treated with careful attention to detail. Splinting and elevation of the hand, early incisional decompression, the institution of early and diligent active motion and expeditious wound closure with skin grafts where full thickness loss has occurred will hasten the time of healing, minimize deformity and assist in the recovery of normal hand function.", "contents": "Skin and soft tissue injuries of the hand in children. Three pediatric hand injury subtypes and their management have been presented. Finger tip injuries, while often minor and self-healing in children, may require surgical repair if the nailbed is disrupted or if there has been significant skin or pad loss from the tip. The decision between local repair and skin graft or pedicle reconstruction depends on the nature and extent of the injury. Multiple digit injuries should be carefully evaluated and manged with a view toward retention of digit length and restoration of function. Immediate closure of all wounds, while desirable, is not necessary. Minor hand burns are quite easily managed but burn injuries more severe in extent and/or depth must be treated with careful attention to detail. Splinting and elevation of the hand, early incisional decompression, the institution of early and diligent active motion and expeditious wound closure with skin grafts where full thickness loss has occurred will hasten the time of healing, minimize deformity and assist in the recovery of normal hand function."}
{"id": "PMID:1091911", "title": "A review: new thoughts on growth.", "content": "There is a critical point in development when the size of an animal, arising from its previous plane of nutrition, determines its appetite thereafter, and hence its rate of growth and dimensions at maturity. A small size at this critical time, brought about by undernutrition, is not followed by \"catch-up\" growth, however liberal the diet. A full diet produces catch-up growth only if the undernutrition, whatever its cause, has occurred after this critical period is over. It can, moreover, only restore a young animal to its percentile channel of growth, and its ability to do this after long periods of undernutrition becomes progressively limited by the animals chronologic age when the catch-up growth became possible.", "contents": "A review: new thoughts on growth. There is a critical point in development when the size of an animal, arising from its previous plane of nutrition, determines its appetite thereafter, and hence its rate of growth and dimensions at maturity. A small size at this critical time, brought about by undernutrition, is not followed by \"catch-up\" growth, however liberal the diet. A full diet produces catch-up growth only if the undernutrition, whatever its cause, has occurred after this critical period is over. It can, moreover, only restore a young animal to its percentile channel of growth, and its ability to do this after long periods of undernutrition becomes progressively limited by the animals chronologic age when the catch-up growth became possible."}
{"id": "PMID:1091914", "title": "[Determination of intracapillary HbO2 saturation with a cryo-microphotometric method, applied to the rabbit myocardium (author's transl)].", "content": "We succeeded in determining the O2 saturation of Hb in capillaries of the rabbit myocardium with a cryo-microphotometric method. Myocardial samples (2-3 mm) were removed in situ with a pair of nitrogin-cooled copper tongs, and rapidly frozen in Freon 13. The samples were sectioned into 10-12 mum slices in a cryotome at about minus 60 degrees C. Hemoglobin absorption spectra were measured on the capillary sections of the frozen slices at minus 100 degrees C in a vacumm-isolated microscope cooling chamber which we developed for the UMSP 1 (C. Zeiss, Oberkochen). The spectra were subdivided into three basic components (oxygenated Hb, desoxygenated Gb, and desoxygenated dehydrated Hb) with a weighted multi-component analysis (L\u00fcbbers and Wodick, 1969). The basic components were measured in Hb solutions. The accuracy of the method was tested both with Hb solutions inserted into glass capillaries, and Hb droplets. O2 saturations of the Hb solutions were measured at room temperature. Subsequently, both test solutions were frozen, HbO2 saturation measured in the glass capillaries at low temperatures agreed well with those recorded at room temperature. HbO2 saturation of the sectioned hemoglobin droplets was found to be systematically increased with low Hb32 saturations as compared with the records made at room temperature. The systematic error was determined and the intracapillary HbO2 saturations were corrected accordingly. Measurements on samples of the rabbit myocardium showed that the histogram of capillary HbO2 saturations has a maximum at saturations of 20-30%. The lowest saturation values were found to be between 0 and 10%, but these values were recorded less frequently. The arterial HbO2 saturation in the femoral artery was about 98%.", "contents": "[Determination of intracapillary HbO2 saturation with a cryo-microphotometric method, applied to the rabbit myocardium (author's transl)]. We succeeded in determining the O2 saturation of Hb in capillaries of the rabbit myocardium with a cryo-microphotometric method. Myocardial samples (2-3 mm) were removed in situ with a pair of nitrogin-cooled copper tongs, and rapidly frozen in Freon 13. The samples were sectioned into 10-12 mum slices in a cryotome at about minus 60 degrees C. Hemoglobin absorption spectra were measured on the capillary sections of the frozen slices at minus 100 degrees C in a vacumm-isolated microscope cooling chamber which we developed for the UMSP 1 (C. Zeiss, Oberkochen). The spectra were subdivided into three basic components (oxygenated Hb, desoxygenated Gb, and desoxygenated dehydrated Hb) with a weighted multi-component analysis (L\u00fcbbers and Wodick, 1969). The basic components were measured in Hb solutions. The accuracy of the method was tested both with Hb solutions inserted into glass capillaries, and Hb droplets. O2 saturations of the Hb solutions were measured at room temperature. Subsequently, both test solutions were frozen, HbO2 saturation measured in the glass capillaries at low temperatures agreed well with those recorded at room temperature. HbO2 saturation of the sectioned hemoglobin droplets was found to be systematically increased with low Hb32 saturations as compared with the records made at room temperature. The systematic error was determined and the intracapillary HbO2 saturations were corrected accordingly. Measurements on samples of the rabbit myocardium showed that the histogram of capillary HbO2 saturations has a maximum at saturations of 20-30%. The lowest saturation values were found to be between 0 and 10%, but these values were recorded less frequently. The arterial HbO2 saturation in the femoral artery was about 98%."}
{"id": "PMID:1091915", "title": "Study of the role of the acceptor stem in the interactions between tRNAs and aminoacyl-tRNA synthetases.", "content": "Several studies have clearly demonstrated that the end of the acceptor stem was a very important area determining the aminoacylation properties of tRNAs. However the attempts to measure the contribution of this region to the binding of tRNAs to aminoacyl-tRNA synthetases have led to contradictory results. We report here the stepwise degradation of yeast tRNA-Phe and tRNA-Val from their 3' terminus, up to the seventh nucleotide : the affinity of each of the degraded-tRNA for their cognate aminoacyl-tRNA synthetase was compared to that of intact tRNA and it was found that these affinities are not significantly decreased when compared to those of the intact tRNAs.", "contents": "Study of the role of the acceptor stem in the interactions between tRNAs and aminoacyl-tRNA synthetases. Several studies have clearly demonstrated that the end of the acceptor stem was a very important area determining the aminoacylation properties of tRNAs. However the attempts to measure the contribution of this region to the binding of tRNAs to aminoacyl-tRNA synthetases have led to contradictory results. We report here the stepwise degradation of yeast tRNA-Phe and tRNA-Val from their 3' terminus, up to the seventh nucleotide : the affinity of each of the degraded-tRNA for their cognate aminoacyl-tRNA synthetase was compared to that of intact tRNA and it was found that these affinities are not significantly decreased when compared to those of the intact tRNAs."}
{"id": "PMID:1091916", "title": "In vivo and in vitro effects of rifampicin and streptolydigin on transcription of Kluyveromyces lactis in the presence of nystatin.", "content": "Rifampicin and streptolydigin, if used in conjunction with nystatin, depress the growth of Kluyveromyces lactis. The incorporation of labeled leucine into protein is inhibited by nystatin whereas the incorporation of labeled uracil into RNA is inhibited by rifampicin in nystatin-treated cells. In order to study the mechanism of inhibition of RNA synthesis we purified by DEAE-Sephadex column chromatography four forms of RNA polymerase from K.lactis cells. The general properties of these enyzmes are similar to those of Saccharomyces cerevisiae and of other eukaryotic RNA polymerases. In particular, enzymes IA, IB and III are more active with poly[d(A-T)] template and Mn-2+ than with native or denatured calf thymus DNA. Enzyme II shows optimal activity with denatured calf thymus DNA and Mn2+. When challenged with native calf thymus DNA all enzymes prefer Mg-2+ as a divalent cation whereas with denatured calf thymus DNA all enzymes are more active with Mn-2+. Enzyme II is inhibited by lambda-amanitin but no enzyme is sensitive to rifampicin and streptolydigin. The inhibition of growth and uracil uptake observed when rifampicin is added to nystatin treated cells is probably not caused by a specific inhibition of transcription.", "contents": "In vivo and in vitro effects of rifampicin and streptolydigin on transcription of Kluyveromyces lactis in the presence of nystatin. Rifampicin and streptolydigin, if used in conjunction with nystatin, depress the growth of Kluyveromyces lactis. The incorporation of labeled leucine into protein is inhibited by nystatin whereas the incorporation of labeled uracil into RNA is inhibited by rifampicin in nystatin-treated cells. In order to study the mechanism of inhibition of RNA synthesis we purified by DEAE-Sephadex column chromatography four forms of RNA polymerase from K.lactis cells. The general properties of these enyzmes are similar to those of Saccharomyces cerevisiae and of other eukaryotic RNA polymerases. In particular, enzymes IA, IB and III are more active with poly[d(A-T)] template and Mn-2+ than with native or denatured calf thymus DNA. Enzyme II shows optimal activity with denatured calf thymus DNA and Mn2+. When challenged with native calf thymus DNA all enzymes prefer Mg-2+ as a divalent cation whereas with denatured calf thymus DNA all enzymes are more active with Mn-2+. Enzyme II is inhibited by lambda-amanitin but no enzyme is sensitive to rifampicin and streptolydigin. The inhibition of growth and uracil uptake observed when rifampicin is added to nystatin treated cells is probably not caused by a specific inhibition of transcription."}
{"id": "PMID:1091917", "title": "Synthesis of pppGpN type dinucleotide derivatives: the 5' end sequence of some RNAs.", "content": "A rapid and simple synthesis of pppGpN type /N equals C, U or A/ diribonucleotide derivatives is described by coupling guanosine 2', 3'-cyclic phosphate 5'-triphosphate with the appropriate ribonucleoside in the presence of ribonuclease T-1.", "contents": "Synthesis of pppGpN type dinucleotide derivatives: the 5' end sequence of some RNAs. A rapid and simple synthesis of pppGpN type /N equals C, U or A/ diribonucleotide derivatives is described by coupling guanosine 2', 3'-cyclic phosphate 5'-triphosphate with the appropriate ribonucleoside in the presence of ribonuclease T-1."}
{"id": "PMID:1091918", "title": "Primary sequence of the 16S ribosomal RNA of Escherichia coli.", "content": "Recent progress in the nucleotide sequence analysis of the 16S ribosomal RNA from E. coli is described. The sequence which has been partially or completely determined so far encompasses 1520 nucleotides, i.e. about 95% of the molecule. Possible features of the secondary structure are suggested on the basis of the nucleotide sequence and data on sequence heterogeneities, repetitions and the location of modified nucleotides are presented. In the accompanying paper, the use of the nucleotide sequence data in studies of the ribosomal protein binding sites is described.", "contents": "Primary sequence of the 16S ribosomal RNA of Escherichia coli. Recent progress in the nucleotide sequence analysis of the 16S ribosomal RNA from E. coli is described. The sequence which has been partially or completely determined so far encompasses 1520 nucleotides, i.e. about 95% of the molecule. Possible features of the secondary structure are suggested on the basis of the nucleotide sequence and data on sequence heterogeneities, repetitions and the location of modified nucleotides are presented. In the accompanying paper, the use of the nucleotide sequence data in studies of the ribosomal protein binding sites is described."}
{"id": "PMID:1091919", "title": "Location and characteristics of ribosomal protein binding sites in the 16S RNA of Escherichia coli.", "content": "Specific binding sites for five proteins of the Escherichia coli 30S ribosomal subunit have been located within the 16S RNA. The sites are structurally diverse and range in size from 40 to 500 nucleotides; their functional integrity appears to depend upon both the secondary structure and conformation of the RNA molecule. Evidence is presented which indicates that additional proteins interact with the RNA at later stages of subunit assembly.", "contents": "Location and characteristics of ribosomal protein binding sites in the 16S RNA of Escherichia coli. Specific binding sites for five proteins of the Escherichia coli 30S ribosomal subunit have been located within the 16S RNA. The sites are structurally diverse and range in size from 40 to 500 nucleotides; their functional integrity appears to depend upon both the secondary structure and conformation of the RNA molecule. Evidence is presented which indicates that additional proteins interact with the RNA at later stages of subunit assembly."}
{"id": "PMID:1091924", "title": "Interpreting cardiac catheterization data. Part 2.", "content": "Data obtained by cardiac catheterization, properly interpreted, are an extremely useful supplement to information obtained from the medical history, physical examination, electrocardiography, chest x-ray examination, and other noninvasive diagnostic techniques. Elevations of pressure in the cardiac chambers and great vessels and corresponding patterns of pressure curves are discerned in the presence of various pathologic conditions affecting the heart. Blood samples are taken during cardiac catheterization for measurement of cardiac output and to detect intracardiac shunting. Attempts have been made to estimate the magnitude of shunts from abnormal dye-dilution curves. Exercise stress is often used during cardiac catheterization to evaluate overall cardiovascular performance. Angiocardiography gives a permanent graphic record of structural abnormalities. Myocardial lactate metabolism provides a means of evaluating the adequacy of coronary blood flow.", "contents": "Interpreting cardiac catheterization data. Part 2. Data obtained by cardiac catheterization, properly interpreted, are an extremely useful supplement to information obtained from the medical history, physical examination, electrocardiography, chest x-ray examination, and other noninvasive diagnostic techniques. Elevations of pressure in the cardiac chambers and great vessels and corresponding patterns of pressure curves are discerned in the presence of various pathologic conditions affecting the heart. Blood samples are taken during cardiac catheterization for measurement of cardiac output and to detect intracardiac shunting. Attempts have been made to estimate the magnitude of shunts from abnormal dye-dilution curves. Exercise stress is often used during cardiac catheterization to evaluate overall cardiovascular performance. Angiocardiography gives a permanent graphic record of structural abnormalities. Myocardial lactate metabolism provides a means of evaluating the adequacy of coronary blood flow."}
{"id": "PMID:1091925", "title": "Problems of aging: sex in the aging.", "content": "For the aged and aging of today, sex is not taboo. With the change in attitudes of younger people toward sex, the elderly are now seeking enrichment of their lives through continued sexual experience. Physicians with knowledge of the physiologic, endocrinologic, and sociologic aspects of sexuality can help to relieve the loneliness and isolation of older people by giving appropriate encouragement and counsel.", "contents": "Problems of aging: sex in the aging. For the aged and aging of today, sex is not taboo. With the change in attitudes of younger people toward sex, the elderly are now seeking enrichment of their lives through continued sexual experience. Physicians with knowledge of the physiologic, endocrinologic, and sociologic aspects of sexuality can help to relieve the loneliness and isolation of older people by giving appropriate encouragement and counsel."}
{"id": "PMID:1091926", "title": "Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12.", "content": "Three genes, mgl A, B, and C, are required for active transport of substrate by the methylgalactose permease of E. coli K12. We report here that only two of these genes are required for substrate translocation, as seen by the ability or inability of isogenic mgl mutants (referred to as Tra+ and Tra minus, respectively) to grow on methyl-beta-D-galactopyranoside, supplied as sole carbon source. Individual mutants of both the Tra+ and Tra minus classes exhibited no detectable intracellular accumulation of methyl-beta-D-galactopyranoside; thus, the Tra+ phenotype cannot be explained by the mutants' levels of residual active transport. The phosphotransferase (Pts), the beta-galactoside (LacY), and the arabinose (Ara E and Ara F) transport systems are not required for substrate translocation by Tra+ cells. The Tra+ phenotype was identified with mutants defective in the mgl B, locus of the galactose-binding protein, by genetic complementation; the Tra minus phenotype was observed with both mgl A and mgl C mutants. The conclusion that the galactose-binding protein is not required for substrate translocation was supported by direct assays of the mgl mutants' binding protein activity. Mutants capable of translocation all showed reduced galactose-binding protein activity; mutants incapable of translocation exhibited binding protein activity equal to that of the mgl+ parent.", "contents": "Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12. Three genes, mgl A, B, and C, are required for active transport of substrate by the methylgalactose permease of E. coli K12. We report here that only two of these genes are required for substrate translocation, as seen by the ability or inability of isogenic mgl mutants (referred to as Tra+ and Tra minus, respectively) to grow on methyl-beta-D-galactopyranoside, supplied as sole carbon source. Individual mutants of both the Tra+ and Tra minus classes exhibited no detectable intracellular accumulation of methyl-beta-D-galactopyranoside; thus, the Tra+ phenotype cannot be explained by the mutants' levels of residual active transport. The phosphotransferase (Pts), the beta-galactoside (LacY), and the arabinose (Ara E and Ara F) transport systems are not required for substrate translocation by Tra+ cells. The Tra+ phenotype was identified with mutants defective in the mgl B, locus of the galactose-binding protein, by genetic complementation; the Tra minus phenotype was observed with both mgl A and mgl C mutants. The conclusion that the galactose-binding protein is not required for substrate translocation was supported by direct assays of the mgl mutants' binding protein activity. Mutants capable of translocation all showed reduced galactose-binding protein activity; mutants incapable of translocation exhibited binding protein activity equal to that of the mgl+ parent."}
{"id": "PMID:1091927", "title": "Methionine and formylmethionine specific tRNAs coded by bacteriophage T5.", "content": "By RNA-DNA hybridization, as well as chemical and chromatographic analysis, evidence is provided that the bacteriophage T5 codes for the synthesis of two isoacceptor methionine transfer RNA species, tRNA-Met and tRNA-f-Met. Because of the differences in chromatographic properties of T5 phage and host methionine tRNAs, the phage tRNA species are readily distinguishable.", "contents": "Methionine and formylmethionine specific tRNAs coded by bacteriophage T5. By RNA-DNA hybridization, as well as chemical and chromatographic analysis, evidence is provided that the bacteriophage T5 codes for the synthesis of two isoacceptor methionine transfer RNA species, tRNA-Met and tRNA-f-Met. Because of the differences in chromatographic properties of T5 phage and host methionine tRNAs, the phage tRNA species are readily distinguishable."}
{"id": "PMID:1091928", "title": "Nature of R-factor replication in the presence of chloramphenicol.", "content": "Covalently closed circular deoxyribonucleic acid molecules of RSF1030, a nonconjugative R-factor, initiate and complete rounds of semiconservative replication in the absence of protein synthesis long after the bacterial chromosome has ceased its replication. RSF1030 replication under these conditions is sensitive to the inhibitor of ribonucleic acid synthesis, rifampicin. The product of this replication, a covalently closed DNA molecule, shows, in contrast to those molecules produced during the replication in a logarithmically growing culture of Escherichia coli, a transition to the open circular form upon treatment with ribonucleases of alkali. Analysis of the product resulting from alkali treatment indicates that there is a single break in one strand of the original circular duplex. This alkali-sensitive site occurs with equal probability in either of the complementary strands. These results are interpreted as a requirement for an RNA primer for the initiation of RSF1030 DNA synthesis and as showing that its removal from the covalently closed molecule is inhibited in the absence of protein synthesis.", "contents": "Nature of R-factor replication in the presence of chloramphenicol. Covalently closed circular deoxyribonucleic acid molecules of RSF1030, a nonconjugative R-factor, initiate and complete rounds of semiconservative replication in the absence of protein synthesis long after the bacterial chromosome has ceased its replication. RSF1030 replication under these conditions is sensitive to the inhibitor of ribonucleic acid synthesis, rifampicin. The product of this replication, a covalently closed DNA molecule, shows, in contrast to those molecules produced during the replication in a logarithmically growing culture of Escherichia coli, a transition to the open circular form upon treatment with ribonucleases of alkali. Analysis of the product resulting from alkali treatment indicates that there is a single break in one strand of the original circular duplex. This alkali-sensitive site occurs with equal probability in either of the complementary strands. These results are interpreted as a requirement for an RNA primer for the initiation of RSF1030 DNA synthesis and as showing that its removal from the covalently closed molecule is inhibited in the absence of protein synthesis."}
{"id": "PMID:1091929", "title": "Spectator-ion effect on the passage of ions through membranes.", "content": "In this paper, we investigate the interplay between geometric and dielectric factors in influencing the image force acting on an ion passing through a membrane, for a system having the approximate dimensions of Escherichia coli. We also study the effect of one ion in a membrane on the passage of a second ion through the membrane, by calculating the radial and angular forces experienced by the second ion due to the presence of the \"spectator ion.\" Our conclusions follow from numerical studies on expressions obtained by solving (exactly) Laplace's equation for the model assumed in this paper. The conclusions are: (i) small changes in the dielectric constant of the membrane are far more significant in determining the image force acting on an ion in a membrane than dramatic changes in the dielectric character of the regions interior and/or exterior to the cell; (ii) a spectator ion in a membrane situated near a boundary may influence in a significant way the passage of a second ion through the middle third of the membrane. We suggest that this latter result should be taken into account in discussing the mechanism of ion migration across membranes.", "contents": "Spectator-ion effect on the passage of ions through membranes. In this paper, we investigate the interplay between geometric and dielectric factors in influencing the image force acting on an ion passing through a membrane, for a system having the approximate dimensions of Escherichia coli. We also study the effect of one ion in a membrane on the passage of a second ion through the membrane, by calculating the radial and angular forces experienced by the second ion due to the presence of the \"spectator ion.\" Our conclusions follow from numerical studies on expressions obtained by solving (exactly) Laplace's equation for the model assumed in this paper. The conclusions are: (i) small changes in the dielectric constant of the membrane are far more significant in determining the image force acting on an ion in a membrane than dramatic changes in the dielectric character of the regions interior and/or exterior to the cell; (ii) a spectator ion in a membrane situated near a boundary may influence in a significant way the passage of a second ion through the middle third of the membrane. We suggest that this latter result should be taken into account in discussing the mechanism of ion migration across membranes."}
{"id": "PMID:1091930", "title": "Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli.", "content": "We studied mutants of E. coli originally identified as being deficient in either endonuclease II (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.30) or exonuclease III [deoxyribonucleate (double-stranded) 5'-nucleotidohydrolase, EC 3.1.4.27] activity. Twelve independently derived mutants were tested, including three new endonuclease II mutants. Deficiency of one enzyme was always accompanied by deficiency of the other. Furthermore, temperature-sensitivity of one activity was always accompanied by temperature-sensitivity of the other, and the enzymes were co-purified. The results suggested a physical association between exonuclease III and endonuclease II, which may be of advantage in the excision-repair of DNA. A thermolabile endonuclease II was purified from one of the new mutants, indicating that it had an altered structural gene. This mutation, and all similar ones mapped by genetic transduction, was located between the pncA and aroD genes on the E. coli chromosome. One mutant had a prolonged generation time, an increased sensitivity to the alkylating agents methyl-methanesulfonate and mitomycin C, and a decreased plating efficiency for bacteriophage lambda, but no marked sensitivity to ultraviolet or gamma-irradiation. Its enzymatic and biological abnormalities were simultaneously revertible, suggesting they were caused by a single mutation. These results suggested a role for these enzymes in normal cell growth processes and in the repair of alkylation damage.", "contents": "Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli. We studied mutants of E. coli originally identified as being deficient in either endonuclease II (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.30) or exonuclease III [deoxyribonucleate (double-stranded) 5'-nucleotidohydrolase, EC 3.1.4.27] activity. Twelve independently derived mutants were tested, including three new endonuclease II mutants. Deficiency of one enzyme was always accompanied by deficiency of the other. Furthermore, temperature-sensitivity of one activity was always accompanied by temperature-sensitivity of the other, and the enzymes were co-purified. The results suggested a physical association between exonuclease III and endonuclease II, which may be of advantage in the excision-repair of DNA. A thermolabile endonuclease II was purified from one of the new mutants, indicating that it had an altered structural gene. This mutation, and all similar ones mapped by genetic transduction, was located between the pncA and aroD genes on the E. coli chromosome. One mutant had a prolonged generation time, an increased sensitivity to the alkylating agents methyl-methanesulfonate and mitomycin C, and a decreased plating efficiency for bacteriophage lambda, but no marked sensitivity to ultraviolet or gamma-irradiation. Its enzymatic and biological abnormalities were simultaneously revertible, suggesting they were caused by a single mutation. These results suggested a role for these enzymes in normal cell growth processes and in the repair of alkylation damage."}
{"id": "PMID:1091931", "title": "Quantitation of the sensory response in bacterial chemotaxis.", "content": "A quantitative assay for the stimulus-response relationship in bacterial chemotaxis has been developed by measurement of tumble frequency. Application of the assay has shown an additive relationship between changes in receptor occupancy and recovery times. Tumble suppression is related to the change in receptor occupancy and not to its rate of change. The results can be explained in terms of varying levels of a tumble regulator.", "contents": "Quantitation of the sensory response in bacterial chemotaxis. A quantitative assay for the stimulus-response relationship in bacterial chemotaxis has been developed by measurement of tumble frequency. Application of the assay has shown an additive relationship between changes in receptor occupancy and recovery times. Tumble suppression is related to the change in receptor occupancy and not to its rate of change. The results can be explained in terms of varying levels of a tumble regulator."}
{"id": "PMID:1091932", "title": "Preferential and cooperative binding of histone I to chromosomal mammalian DNA.", "content": "There is a strong preferential binding of histone I to lymphocyte DNA as compared to Escherichia coli DNA when large DNA fragments (2 times 10-6 daltons) are used. The binding of histone I to lymphocyte DNA and to E. coli DNA is cooperative. The distribution of preferential binding sites has been investigated on fragmented DNA. Most of the 2 times 10-6 dalton fragments were found to have at least one preferential histone I binding site, whereas most of the 2 times 10-5 dalton fragments have none.", "contents": "Preferential and cooperative binding of histone I to chromosomal mammalian DNA. There is a strong preferential binding of histone I to lymphocyte DNA as compared to Escherichia coli DNA when large DNA fragments (2 times 10-6 daltons) are used. The binding of histone I to lymphocyte DNA and to E. coli DNA is cooperative. The distribution of preferential binding sites has been investigated on fragmented DNA. Most of the 2 times 10-6 dalton fragments were found to have at least one preferential histone I binding site, whereas most of the 2 times 10-5 dalton fragments have none."}
{"id": "PMID:1091933", "title": "Immunosuppression in vitro induced by leukemia virus-infected splenocytes (38544).", "content": "Immunization of dispersed spleen cells from normal mice in vitro with SRBC was suppressed by simultaneous incubation of the spleen cell cultures with splenocytes from mice previously infected with FLV. Cell-free virus preparations alone did not suppress the antibody response. In contrast, relatively small numbers of splenocytes from infected mice, even when present at a ratio of 1-500 normal spleen cells, significantly suppressed the in vitro immune response to SRBC. Viable leukemic splenocytes were necessary for immunosuppression although the leukemic cells did not have to be in direct contact with the normal spleen cells. Specific anti-FLV serum, when added to the leukemic splenocytes or to normal spleen cells separated from infected cells by cell-impermeable membranes, prevented immunodepression.", "contents": "Immunosuppression in vitro induced by leukemia virus-infected splenocytes (38544). Immunization of dispersed spleen cells from normal mice in vitro with SRBC was suppressed by simultaneous incubation of the spleen cell cultures with splenocytes from mice previously infected with FLV. Cell-free virus preparations alone did not suppress the antibody response. In contrast, relatively small numbers of splenocytes from infected mice, even when present at a ratio of 1-500 normal spleen cells, significantly suppressed the in vitro immune response to SRBC. Viable leukemic splenocytes were necessary for immunosuppression although the leukemic cells did not have to be in direct contact with the normal spleen cells. Specific anti-FLV serum, when added to the leukemic splenocytes or to normal spleen cells separated from infected cells by cell-impermeable membranes, prevented immunodepression."}
{"id": "PMID:1091934", "title": "Effect of a long-acting estrogen on the pituitary response to LH-releasing hormone (38582).", "content": "The effect of Quinestrol, an estrogenic substance of long-lasting activity, on the pituitary responsiveness to LH-RH was studied in ovariectomized rats.  Quinestrol administered as a single 200 pg-dose significantly augmented the pituitary response to LH-RH 36 hr, 9 days, and although not significantly, also at 24 days after its oral administration.  No effect could be detected 36 days after its administration.  At a 20 pg-dose Quinestrol significantly augmented the pituitary response to LH-RH only at the 36 hr-period studied, this effect not being apparent in later time periods.", "contents": "Effect of a long-acting estrogen on the pituitary response to LH-releasing hormone (38582). The effect of Quinestrol, an estrogenic substance of long-lasting activity, on the pituitary responsiveness to LH-RH was studied in ovariectomized rats.  Quinestrol administered as a single 200 pg-dose significantly augmented the pituitary response to LH-RH 36 hr, 9 days, and although not significantly, also at 24 days after its oral administration.  No effect could be detected 36 days after its administration.  At a 20 pg-dose Quinestrol significantly augmented the pituitary response to LH-RH only at the 36 hr-period studied, this effect not being apparent in later time periods."}
{"id": "PMID:1091935", "title": "An in vivo study of an antimicrobial mouth rinse on supragingival and subgingival plaque and calculus formation.", "content": "An antibiotic mouth rinse (CC10232) was used twice a day for one week in human subjects in a supervised double blind clinical and histological study. Nylon strips were placed supra and subgingivally for one week and then sectioned, stained and examined. Results showed that the rinse containing CC-10232 reduced the amount of plaque and reduced the amount of mineralization in 4 of 5 subjects.Also, no spirochetes were found in subjects who utilized this rinse.", "contents": "An in vivo study of an antimicrobial mouth rinse on supragingival and subgingival plaque and calculus formation. An antibiotic mouth rinse (CC10232) was used twice a day for one week in human subjects in a supervised double blind clinical and histological study. Nylon strips were placed supra and subgingivally for one week and then sectioned, stained and examined. Results showed that the rinse containing CC-10232 reduced the amount of plaque and reduced the amount of mineralization in 4 of 5 subjects.Also, no spirochetes were found in subjects who utilized this rinse."}
{"id": "PMID:1091939", "title": "A comparison of circling models for the detection of antiparkinson activity.", "content": "Apomorphine, d-amphetamine, methylphenidate, nomifensine, ET495 and amantadine each induced a dose-dependent stereotyped behaviour in the rat. L-Dopa was inactive in the absence of any pretreatment. The behaviour induced by apomorphine, methylphenidate, nomifensine and amantadine persisted following pretreatment with a-methylparatyrosine. However, only the effect of apomorphine developed following combined pretreatment with reserpine/a-methylparatyrosine. The effects of all stereotypic agents were inhibited by haloperidol, apomorphine, methylphenidate and nomifensine being most resistant. All agents, including L-Dopa, induced a dose-dependent contralateral circling behaviour in animals with asymmetric lesion of the medial raph\u00e9 nucleus and a dose-dependent ipsilateral circling after unilateral lesion of the substantia nigra. Following unilateral lesions in the ventromedial area of the medial forebrain bundle (medial to the substantia nigra and carrying 5-hydroxytryptamine neurones) amphetamine caused an ipsilateral circling behaviour, amantadine a behaviour which was characterised by definite bursts in either direction, and all other agents caused contralateral circling.", "contents": "A comparison of circling models for the detection of antiparkinson activity. Apomorphine, d-amphetamine, methylphenidate, nomifensine, ET495 and amantadine each induced a dose-dependent stereotyped behaviour in the rat. L-Dopa was inactive in the absence of any pretreatment. The behaviour induced by apomorphine, methylphenidate, nomifensine and amantadine persisted following pretreatment with a-methylparatyrosine. However, only the effect of apomorphine developed following combined pretreatment with reserpine/a-methylparatyrosine. The effects of all stereotypic agents were inhibited by haloperidol, apomorphine, methylphenidate and nomifensine being most resistant. All agents, including L-Dopa, induced a dose-dependent contralateral circling behaviour in animals with asymmetric lesion of the medial raph\u00e9 nucleus and a dose-dependent ipsilateral circling after unilateral lesion of the substantia nigra. Following unilateral lesions in the ventromedial area of the medial forebrain bundle (medial to the substantia nigra and carrying 5-hydroxytryptamine neurones) amphetamine caused an ipsilateral circling behaviour, amantadine a behaviour which was characterised by definite bursts in either direction, and all other agents caused contralateral circling."}
{"id": "PMID:1091940", "title": "Propranolol in the treatment of opiate dependence -- a controlled study.", "content": "Two doublebling placebo controlled experiments designed to elucidate the alleged narcotic blocking effect of propranolol wer performed. In the first experiment, propranolol 40 mg or 20 mg was given together with methadone during the acute withdrawal phase of opiate addiction. The second experiment assessed whether 10 mg of propranolol, given 2 hrs before 30 mg of morphine i.v., reduced the euphoric effects of the latter drug. In none of the experiments could narcotic blocking effects be detected. The group receiving 40 mg propranolol during detoxification exhibited the highest proportion of patients staying for the whole prescribed detoxification period.", "contents": "Propranolol in the treatment of opiate dependence -- a controlled study. Two doublebling placebo controlled experiments designed to elucidate the alleged narcotic blocking effect of propranolol wer performed. In the first experiment, propranolol 40 mg or 20 mg was given together with methadone during the acute withdrawal phase of opiate addiction. The second experiment assessed whether 10 mg of propranolol, given 2 hrs before 30 mg of morphine i.v., reduced the euphoric effects of the latter drug. In none of the experiments could narcotic blocking effects be detected. The group receiving 40 mg propranolol during detoxification exhibited the highest proportion of patients staying for the whole prescribed detoxification period."}
{"id": "PMID:1091946", "title": "[View of the past: Roentgenography of an Egyptian mummy].", "content": "Report concerning roentgenographic examinations of an Egyptian mummy dating back to 900 B.C. from the \"Museum f\u00fcr V\u00f6lkerkunde Freiburg/Breisgau\". The study was performed to detect funeral gifts which can be found otherwise only if the linnen bandage of the mummy is destroyed. Extraordinary findings were degenerative osteoarthritis of the spine and joints, pseudo-gout, marked caries of several teeth and signs for a healed occipital osteomyelitis. Suggestions are discussed about a particular compression of thorax and pelvis which is apparently due to the technique of bandaging. This was not observed in examinations of other mummies. Fractures of the first to third phalanges suggest forceful removal of precious rings. A circular-shaped opening in the parietal bone and a small radiodense foreign body which projects over the pharynx remain unexplained.", "contents": "[View of the past: Roentgenography of an Egyptian mummy]. Report concerning roentgenographic examinations of an Egyptian mummy dating back to 900 B.C. from the \"Museum f\u00fcr V\u00f6lkerkunde Freiburg/Breisgau\". The study was performed to detect funeral gifts which can be found otherwise only if the linnen bandage of the mummy is destroyed. Extraordinary findings were degenerative osteoarthritis of the spine and joints, pseudo-gout, marked caries of several teeth and signs for a healed occipital osteomyelitis. Suggestions are discussed about a particular compression of thorax and pelvis which is apparently due to the technique of bandaging. This was not observed in examinations of other mummies. Fractures of the first to third phalanges suggest forceful removal of precious rings. A circular-shaped opening in the parietal bone and a small radiodense foreign body which projects over the pharynx remain unexplained."}
{"id": "PMID:1091942", "title": "Alcohol and affect in dyadic social interaction.", "content": "The effects of alcohol on emotional behavior during social interaction were studied in an experiment utilizing unstructured discussions between male-female couples. All subjects participated in an alcohol (1.0 ml/kg) and placebo session, and a small number took part in a third higher dose session (1.5 ml/kg). Quantitative and qualitative ratings of affect were made from the recorded interactions. Alcohol produced significant increases in the affects of elation--giddiness and happiness--and an overall increase in total emotional expression. Only some aspects of anxiety were affected by alcohol. Hostility and aggressiveness were not increased on two quantitative measures but showed a significant increase on qualitative scores in the low dose session. Although there was no consistent relationship between blood alcohol level and emotional response , drinking history significantly altered emotional response to alcohol (tolerance effects). The euphoric effects of alcohol that we found are discussed in terms of the unique aspects of the experimental situation employed. Some important variable are delineated that may influence responses to alcohol.", "contents": "Alcohol and affect in dyadic social interaction. The effects of alcohol on emotional behavior during social interaction were studied in an experiment utilizing unstructured discussions between male-female couples. All subjects participated in an alcohol (1.0 ml/kg) and placebo session, and a small number took part in a third higher dose session (1.5 ml/kg). Quantitative and qualitative ratings of affect were made from the recorded interactions. Alcohol produced significant increases in the affects of elation--giddiness and happiness--and an overall increase in total emotional expression. Only some aspects of anxiety were affected by alcohol. Hostility and aggressiveness were not increased on two quantitative measures but showed a significant increase on qualitative scores in the low dose session. Although there was no consistent relationship between blood alcohol level and emotional response , drinking history significantly altered emotional response to alcohol (tolerance effects). The euphoric effects of alcohol that we found are discussed in terms of the unique aspects of the experimental situation employed. Some important variable are delineated that may influence responses to alcohol."}
{"id": "PMID:1091954", "title": "The effects of plumbous ion on protein biosynthesis in reticulocytes.", "content": "Plumbous ion a potent inhibitor of hemoglobin synthesis by reticulocytes in vivo, is not as effective in inhibiting in vitro globin synthesizing systems prepared from these cells. The reticulocyte is considerably more sensitive to Pb2+ than are leukemic leukocytes, HeLa cells or bacteria. In fact, protein synthesis in leukemic leukocytes is actually stimulated by Pb2+. The synthesis of non-heme protein as well as hemoglobin is inhibited by Pb2+ in reticulocytes and the synthesis of alpha chains is inhibited to a greater degree tcids into reticulocytes. All of these observations are consistent with the biosynthesis of heme rather than any of the steps in protein biosynthesis being the locus for inhibition of hemoglobin synthesis by low levels of plumbous ion. Also, there is marked biological variations in the susceptibilities of reticulocytes from different rabbits to Pb2+.", "contents": "The effects of plumbous ion on protein biosynthesis in reticulocytes. Plumbous ion a potent inhibitor of hemoglobin synthesis by reticulocytes in vivo, is not as effective in inhibiting in vitro globin synthesizing systems prepared from these cells. The reticulocyte is considerably more sensitive to Pb2+ than are leukemic leukocytes, HeLa cells or bacteria. In fact, protein synthesis in leukemic leukocytes is actually stimulated by Pb2+. The synthesis of non-heme protein as well as hemoglobin is inhibited by Pb2+ in reticulocytes and the synthesis of alpha chains is inhibited to a greater degree tcids into reticulocytes. All of these observations are consistent with the biosynthesis of heme rather than any of the steps in protein biosynthesis being the locus for inhibition of hemoglobin synthesis by low levels of plumbous ion. Also, there is marked biological variations in the susceptibilities of reticulocytes from different rabbits to Pb2+."}
{"id": "PMID:1091959", "title": "Comparison of two steroid preparations used to treat tennis elbow, using the hypospray.", "content": "Methyl prednisolone acetate and hydrocortisone acetate were compared in a trial using the Hypospray to treat tennis elbow. There was little difference between the two preparations as far as side-effects and short-term suppression of symptoms were concerned, both being successful. However, late recurrence was very common in both groups, suggesting that a reappraisal of local steroid in this condition is needed.", "contents": "Comparison of two steroid preparations used to treat tennis elbow, using the hypospray. Methyl prednisolone acetate and hydrocortisone acetate were compared in a trial using the Hypospray to treat tennis elbow. There was little difference between the two preparations as far as side-effects and short-term suppression of symptoms were concerned, both being successful. However, late recurrence was very common in both groups, suggesting that a reappraisal of local steroid in this condition is needed."}
{"id": "PMID:1091962", "title": "A comparison of stable and 3H- labelled polyethylene glycol 4000 as non-absorbable water phase markers in the human ileum and faeces.", "content": "Polyethylene glycol  4000 (PEG plus 3H-PEG) was used as volume indicator during ileal perfusion in man. Faeces, urine, ileal infusates, and aspirates were investigated. Turbidimetrical assay and liquid scintillation counting (in the faeces radioassay was done after conbustion) yielded similar estimates of water absorption and secretion. Tracer amounts of 3H were found in the urine, indicating a minimal absorption. The simplicity of 3H radioassay and the reliability of 3H-PEG as a non-absorbable water phase marker is emphasized.", "contents": "A comparison of stable and 3H- labelled polyethylene glycol 4000 as non-absorbable water phase markers in the human ileum and faeces. Polyethylene glycol  4000 (PEG plus 3H-PEG) was used as volume indicator during ileal perfusion in man. Faeces, urine, ileal infusates, and aspirates were investigated. Turbidimetrical assay and liquid scintillation counting (in the faeces radioassay was done after conbustion) yielded similar estimates of water absorption and secretion. Tracer amounts of 3H were found in the urine, indicating a minimal absorption. The simplicity of 3H radioassay and the reliability of 3H-PEG as a non-absorbable water phase marker is emphasized."}
{"id": "PMID:1091963", "title": "Studies of gastrointestinal interactions. VII. Characteristics of the absorption pattern of sugar, fat and protein from composite meals in man. A quantitative study.", "content": "The absorption of sugar, fat, and protein after feeding two meals, differing in their content of glucose, was investigated in 7 healthy subjects, in double experiments with the multiple indicator dilution method. An average of 75% of the emptied amounts of sugar and 80% of the emptied fat and protein was absorbed during transit of the proximal 70 cm of the intestine. Differences in the absorption pattern between the meals were recorded, resulting from their different intragastric behaviour and gastric emptying pattern. Within the first hour significantly higher amounts of fat and protein were absorbed after the glucose-free than after the glucose-containing meal, whereas from the latter most of the glucose was absorbed early after ingestion. The absorptive capacity of the investigated segment was not exceeded for any component of the meals, owing to compensatory mechanisms by which the gastric emptying rate was inhibited and the transit time through the segment prolonged. The efficiency of sugar absorption was related to the transit time through the segment, in that a constant fraction was eliminated from the intestinal lumen per minute increase of the mean transit time. The net endogenous contribution of protein and fat at the exit of the segment amounted to an average of 3.0 g protein and 1.2 g fat during 3 hours.", "contents": "Studies of gastrointestinal interactions. VII. Characteristics of the absorption pattern of sugar, fat and protein from composite meals in man. A quantitative study. The absorption of sugar, fat, and protein after feeding two meals, differing in their content of glucose, was investigated in 7 healthy subjects, in double experiments with the multiple indicator dilution method. An average of 75% of the emptied amounts of sugar and 80% of the emptied fat and protein was absorbed during transit of the proximal 70 cm of the intestine. Differences in the absorption pattern between the meals were recorded, resulting from their different intragastric behaviour and gastric emptying pattern. Within the first hour significantly higher amounts of fat and protein were absorbed after the glucose-free than after the glucose-containing meal, whereas from the latter most of the glucose was absorbed early after ingestion. The absorptive capacity of the investigated segment was not exceeded for any component of the meals, owing to compensatory mechanisms by which the gastric emptying rate was inhibited and the transit time through the segment prolonged. The efficiency of sugar absorption was related to the transit time through the segment, in that a constant fraction was eliminated from the intestinal lumen per minute increase of the mean transit time. The net endogenous contribution of protein and fat at the exit of the segment amounted to an average of 3.0 g protein and 1.2 g fat during 3 hours."}
{"id": "PMID:1091964", "title": "In vitro pathogenetic studies of coeliac disease. Effects of protein digests on coeliac intestinal biopsy specimens maintained in culture for 48 hours.", "content": "Jos, J., Lenoir, G., de Ritis, G. & Rey, J. In vitro pathogenetic studies of coeliac disease. Effects of protein digests on coeliac intestinal biopsy specimens maintained in culture for 48 hours. Scand. J. Gastroent. 1975, 10, 121-128. Intestinal biopsies from controls and from children with treated or untreated coeliac disease were cultured for 48 hours in the presence or absence of various peptic-tryptic (P.T.) or peptic-tryptic-chymotryptic (P.T.C.) digests of gliadin and casein, using a modified organ culture method. In flat biopsy specimens obtained from children with active coeliac disease and maintained in culture in the presence of P.T. or P.T.C. digests of gliadin, whether autoclaved or not, a cytotoxic effect was obvious; but this effect was in part unspecific, since P.T. digests of casein were also slightly noxious in such experimental conditions. Biopsies from controls or from children with coeliac disease in remission were not affected by the presence in culture medium of casein or gliadin digests, whereas coeliac biopsies, also obtained from patients in remission but after gluten challenge, were specifically injured during culture with gliadin peptides. On the other hand, a thorough P.T.C. hydrolysis of gliadin abolished all its in vitro noxious effects, suggesting that its toxicity is related to a relatively large peptide.", "contents": "In vitro pathogenetic studies of coeliac disease. Effects of protein digests on coeliac intestinal biopsy specimens maintained in culture for 48 hours. Jos, J., Lenoir, G., de Ritis, G. & Rey, J. In vitro pathogenetic studies of coeliac disease. Effects of protein digests on coeliac intestinal biopsy specimens maintained in culture for 48 hours. Scand. J. Gastroent. 1975, 10, 121-128. Intestinal biopsies from controls and from children with treated or untreated coeliac disease were cultured for 48 hours in the presence or absence of various peptic-tryptic (P.T.) or peptic-tryptic-chymotryptic (P.T.C.) digests of gliadin and casein, using a modified organ culture method. In flat biopsy specimens obtained from children with active coeliac disease and maintained in culture in the presence of P.T. or P.T.C. digests of gliadin, whether autoclaved or not, a cytotoxic effect was obvious; but this effect was in part unspecific, since P.T. digests of casein were also slightly noxious in such experimental conditions. Biopsies from controls or from children with coeliac disease in remission were not affected by the presence in culture medium of casein or gliadin digests, whereas coeliac biopsies, also obtained from patients in remission but after gluten challenge, were specifically injured during culture with gliadin peptides. On the other hand, a thorough P.T.C. hydrolysis of gliadin abolished all its in vitro noxious effects, suggesting that its toxicity is related to a relatively large peptide."}
{"id": "PMID:1091965", "title": "Acetylsalicylic acid and gastrointestinal bleeding measurement of blood loss using a modified radioactive chromium method.", "content": "A comparative study of the gastrointestinal blood loss during intake of a new buffered acetylsalicylic acid (ASA) preparation and plain ASA tablets has been performed. A modified radioactive chromium method was used. The 51Cr activity was considerably less than that used in previous methods, and no feces homogenization was necessary. The study was performed in 18 volunteers using a randomized crossover design. A significant reduction in gastrointestinal bleeding was registered during ingestion of the buffered ASA in comparison with the plain ASA tablets.", "contents": "Acetylsalicylic acid and gastrointestinal bleeding measurement of blood loss using a modified radioactive chromium method. A comparative study of the gastrointestinal blood loss during intake of a new buffered acetylsalicylic acid (ASA) preparation and plain ASA tablets has been performed. A modified radioactive chromium method was used. The 51Cr activity was considerably less than that used in previous methods, and no feces homogenization was necessary. The study was performed in 18 volunteers using a randomized crossover design. A significant reduction in gastrointestinal bleeding was registered during ingestion of the buffered ASA in comparison with the plain ASA tablets."}
{"id": "PMID:1091966", "title": "Effects of 13-nle-motilin in man--inhibition of gastric evacuation and stimulation of pepsin secretion.", "content": "In 3 healthy male volunteers, graded doses of 13-norleucine-motilin (13-nle-motilin)--synthetic analogue of motilin and biologically equivalent to the natural polypeptide--were given intravenously on separate days. Gastric emptying, as determined by means of a radioisotope method, was slowed down dose-dependently by increasing doses of 13-nle-motilin. This was indicated by increasing \"half-lives\" and \"starting indices\" of the gastric emptying process, and by decreasing pyloric loss rates, respectively. The gastric pepsin output rose markedly from the basal level during intravenous infusion of 13-nle-motilin.", "contents": "Effects of 13-nle-motilin in man--inhibition of gastric evacuation and stimulation of pepsin secretion. In 3 healthy male volunteers, graded doses of 13-norleucine-motilin (13-nle-motilin)--synthetic analogue of motilin and biologically equivalent to the natural polypeptide--were given intravenously on separate days. Gastric emptying, as determined by means of a radioisotope method, was slowed down dose-dependently by increasing doses of 13-nle-motilin. This was indicated by increasing \"half-lives\" and \"starting indices\" of the gastric emptying process, and by decreasing pyloric loss rates, respectively. The gastric pepsin output rose markedly from the basal level during intravenous infusion of 13-nle-motilin."}
{"id": "PMID:1091967", "title": "Gastric secretory inhibition by two 16-methylated analogs of prostaglandin E2 given intragastrically to patients with duodenal ulcer disease.", "content": "The effects of two methylated analogs of prostaglandin E2 (PGE2), namely 16,16-dimethyl PGE2 and its methyl ester, on gastric acid and pepsin secretion have been studied in 5 ulcer patients. Both analogs produced significant inhibition of acid secretion in a dose-dependent fashion, but the effect was less pronounced than the inhibition found in a group of healthy volunteers, and it was necessary to give 140 mug of each analog to reach about the ED50. The mean values of pepsin output and pepsin concentration were decreased following administration of PGE2, but the individual results varied.", "contents": "Gastric secretory inhibition by two 16-methylated analogs of prostaglandin E2 given intragastrically to patients with duodenal ulcer disease. The effects of two methylated analogs of prostaglandin E2 (PGE2), namely 16,16-dimethyl PGE2 and its methyl ester, on gastric acid and pepsin secretion have been studied in 5 ulcer patients. Both analogs produced significant inhibition of acid secretion in a dose-dependent fashion, but the effect was less pronounced than the inhibition found in a group of healthy volunteers, and it was necessary to give 140 mug of each analog to reach about the ED50. The mean values of pepsin output and pepsin concentration were decreased following administration of PGE2, but the individual results varied."}
{"id": "PMID:1091968", "title": "Needle-like crystals in plasma cells in a patient with a plasma cell proliferative disorder.", "content": "A patient with a plasma cell proliferative disorder and a monoclonal IgG-kappa protein in the serum was observed over a period of four years. Two unusual features were seen in this patient: (1) An apparently benign course in spite of relatively large and slowly increasing amounts of Bence Jones protein (monoclonal kappa light chains) in the urine, and (2) crystalline structures located outside the cisternae of the endoplasmic reticulum in a large number of bone marrow plasma cells. The crystals, which ultrastructurally appeared to be composed of protein material, were not stained by immunofluorescent antisera to immunoglobulins. They were, however, observed only in cells staining for gamma heavy and kappa light chains. This suggest an association between the crystalline structures and the synthesis of monoclonal immunoglobulin proteins.", "contents": "Needle-like crystals in plasma cells in a patient with a plasma cell proliferative disorder. A patient with a plasma cell proliferative disorder and a monoclonal IgG-kappa protein in the serum was observed over a period of four years. Two unusual features were seen in this patient: (1) An apparently benign course in spite of relatively large and slowly increasing amounts of Bence Jones protein (monoclonal kappa light chains) in the urine, and (2) crystalline structures located outside the cisternae of the endoplasmic reticulum in a large number of bone marrow plasma cells. The crystals, which ultrastructurally appeared to be composed of protein material, were not stained by immunofluorescent antisera to immunoglobulins. They were, however, observed only in cells staining for gamma heavy and kappa light chains. This suggest an association between the crystalline structures and the synthesis of monoclonal immunoglobulin proteins."}
{"id": "PMID:1091969", "title": "[Treatment of psoriasis with an orally administered effective new vitamin A acid derivative. Preliminary report].", "content": "The retinoic acid derivative Ro 10-9359 has been tested in 24 patients with psoriasis. In all patients the therapeutic activity was good to excellent. The symptoms of the hypervitaminosis A syndrome were slight to marked. The ratio between the therapeutic effectiveness and the side effects was favourable only in cases with severe psoriasis.", "contents": "[Treatment of psoriasis with an orally administered effective new vitamin A acid derivative. Preliminary report]. The retinoic acid derivative Ro 10-9359 has been tested in 24 patients with psoriasis. In all patients the therapeutic activity was good to excellent. The symptoms of the hypervitaminosis A syndrome were slight to marked. The ratio between the therapeutic effectiveness and the side effects was favourable only in cases with severe psoriasis."}
{"id": "PMID:1091970", "title": "[Leukocyte transfusion. An overview].", "content": "Today the increased risk of infection in patients with transient severe neutropenia can be reduced effectively by three different approaches:1.gnotobiotic patient isolation with whole body decontamination by non-resorbable antibiotics;2. treatment of documented or suspected infection with a conbination of bactericidal antibiotics and/or antimycotics; 3. granulocyte transfusions in the case of life-threatening antibiotic-resistant infections. This third therapeutic approach against severe resistant infection is proving increasingly effective and cannot at present be replaced by different therapeutic measures. The efficiency of granulocyte transfusions depends on: 1. substitution of at least 1 X 10-10 granulocytes per transfusions; 2. repeated transfusions of granulocytes within several consecutive days in the event of only partial success; 3. the transfused granulocytes should not be immediately destroyed in the recipient by preformed antibodies, a factor which makes donor-recipient selection a vital part of the granulocyte replacement program. Severe complications of granulocyte transfusion can be minimized by slow application of the transfused cells. The required number of at least 1 X 10-10 granulocytes for effective transfusion can be collected without significant risk from healthy donors by means of either the \"continuous flow centrifuge\" (CFC) or the technique of \"filtration leukapheresis' (FL). Filtration leukapheresis offers the advantage of a simple collection procedure and much lower cost, coupled with the same numerical and functional effectiveness of cell collection and therapeutic results. Since after transfusion vital granulocytes accumulate in the marginal neutrophil pool, recording of the \"post-transfusion increment\" is not an accurate parameter of transfusion effectiveness. There is marked accumulation of transfused granulocytes in areas of infection.", "contents": "[Leukocyte transfusion. An overview]. Today the increased risk of infection in patients with transient severe neutropenia can be reduced effectively by three different approaches:1.gnotobiotic patient isolation with whole body decontamination by non-resorbable antibiotics;2. treatment of documented or suspected infection with a conbination of bactericidal antibiotics and/or antimycotics; 3. granulocyte transfusions in the case of life-threatening antibiotic-resistant infections. This third therapeutic approach against severe resistant infection is proving increasingly effective and cannot at present be replaced by different therapeutic measures. The efficiency of granulocyte transfusions depends on: 1. substitution of at least 1 X 10-10 granulocytes per transfusions; 2. repeated transfusions of granulocytes within several consecutive days in the event of only partial success; 3. the transfused granulocytes should not be immediately destroyed in the recipient by preformed antibodies, a factor which makes donor-recipient selection a vital part of the granulocyte replacement program. Severe complications of granulocyte transfusion can be minimized by slow application of the transfused cells. The required number of at least 1 X 10-10 granulocytes for effective transfusion can be collected without significant risk from healthy donors by means of either the \"continuous flow centrifuge\" (CFC) or the technique of \"filtration leukapheresis' (FL). Filtration leukapheresis offers the advantage of a simple collection procedure and much lower cost, coupled with the same numerical and functional effectiveness of cell collection and therapeutic results. Since after transfusion vital granulocytes accumulate in the marginal neutrophil pool, recording of the \"post-transfusion increment\" is not an accurate parameter of transfusion effectiveness. There is marked accumulation of transfused granulocytes in areas of infection."}
{"id": "PMID:1091975", "title": "Disorders of hemostatic function in patients with systemic lupus erythematosus.", "content": "A wide spectrum of hemostatic abnormalities is found in patients with SLE. Thrombocytopenia and qualitative platelet disorder (impaired aggregation to collagen) are probably both due to antiplatelet antibodies, which can be found in most patients with the disease. About 10% of patients with SLE have a circulating anticoagulant. These circulating anticoagulants are broadly heterogeneous. Although most reported cases act at the level of the prothrombin converting complex, 15 of the 74 cases here reviewed had other points of action. The anticoagulants are probably all antibodies; they differ (with rare exceptions) from other naturally occurring circulating anticoagulants in having an immediate rather than a progressive effect, and in acting, not against pre-existing procoagulants, but against unstable complexes. An anticoagulant of the type found in SLE is only rarely observed in the absence of SLE; its presence in a patient is thus of some diagnostic importance. Hypoprothrombinemia is a common second lesion in patients with circulating anticoagulants. Its pathogenesis is obscure. Two patients with acquired von Willebrand's disease have been observed. All the hemostatic abnormalities found in SLE probably have immunologic bases; all respond to glucocorticoid treatment.", "contents": "Disorders of hemostatic function in patients with systemic lupus erythematosus. A wide spectrum of hemostatic abnormalities is found in patients with SLE. Thrombocytopenia and qualitative platelet disorder (impaired aggregation to collagen) are probably both due to antiplatelet antibodies, which can be found in most patients with the disease. About 10% of patients with SLE have a circulating anticoagulant. These circulating anticoagulants are broadly heterogeneous. Although most reported cases act at the level of the prothrombin converting complex, 15 of the 74 cases here reviewed had other points of action. The anticoagulants are probably all antibodies; they differ (with rare exceptions) from other naturally occurring circulating anticoagulants in having an immediate rather than a progressive effect, and in acting, not against pre-existing procoagulants, but against unstable complexes. An anticoagulant of the type found in SLE is only rarely observed in the absence of SLE; its presence in a patient is thus of some diagnostic importance. Hypoprothrombinemia is a common second lesion in patients with circulating anticoagulants. Its pathogenesis is obscure. Two patients with acquired von Willebrand's disease have been observed. All the hemostatic abnormalities found in SLE probably have immunologic bases; all respond to glucocorticoid treatment."}
{"id": "PMID:1091976", "title": "Recent observations on central nervous system lupus erythematosus.", "content": "The recent literature on CNS-SLE has been reviewed. An improved prognosis is noted that is thought to be due to the use of high-dose corticosteroids. The frequencies of the various neurologic and psychiatric findings are discussed, and a distinction is noted between organic psychoses and functional psychiatric complaints. The question of corticosteroids versus cerebral vasculitis as the cause of the neuropsychiatric symptomatology in SLE is examined, and the necessity of clear psychiatric diagnosis and treatment is stressed. Recent observations on HL-A antigens, complement, immunoglobulins, virus, and immunocomplexes suggest that the latter are prominent in CNS-SLE, but that an infectious agent may be etiologic in the genesis of SLE. Fifty-four patients not previously reported are discussed. Thirty-eight of them had neuropsychiatric manifestations. The treatment of CNS-SLE with cytotoxic agents, in addition to corticosteroids, is considered, and the experience of the authors with such treatment is presented.", "contents": "Recent observations on central nervous system lupus erythematosus. The recent literature on CNS-SLE has been reviewed. An improved prognosis is noted that is thought to be due to the use of high-dose corticosteroids. The frequencies of the various neurologic and psychiatric findings are discussed, and a distinction is noted between organic psychoses and functional psychiatric complaints. The question of corticosteroids versus cerebral vasculitis as the cause of the neuropsychiatric symptomatology in SLE is examined, and the necessity of clear psychiatric diagnosis and treatment is stressed. Recent observations on HL-A antigens, complement, immunoglobulins, virus, and immunocomplexes suggest that the latter are prominent in CNS-SLE, but that an infectious agent may be etiologic in the genesis of SLE. Fifty-four patients not previously reported are discussed. Thirty-eight of them had neuropsychiatric manifestations. The treatment of CNS-SLE with cytotoxic agents, in addition to corticosteroids, is considered, and the experience of the authors with such treatment is presented."}
{"id": "PMID:1091977", "title": "The surface chemistry of bone mineral and related calcium phosphates.", "content": "A review of the surface chemistry of bone mineral, hydroxyapatite and amorphous calcium phosphate is presented. Small-angle x-ray scattering and low-temperature nitrogen adsorption measurements show the magnitude of bone mineral surface to range from 100-200 m-2/g; the synthetic hydroxyapatite surface can vary from 25-200 m-2/g, while synthetic amorphous calcium phosphate ranges in surface from 20-60 m-2/g, according to the respective preparation conditions. The magnitude of heats of adsorption of certain small molecules (CO, Ar, N2, H2O, CH3OH) on bone mineral and hydroxyapatite show that these are polarizing surfaces that form strong bonds with polar or polarizable molecules; water is hydrogen-bonded to these surfaces with energies ranging from 23 kcal/mole for low coverage to 11 kcal/mole after two full monolayers; concomitantly, methanol ranges from 24 kcal/mole to 9 kcal/mole after the adsorption of one and a half monolayers. Stearic acid will close-pack perpendicularly on bone apatite surfaces when adsorbed from cyclohexane solution in a way reminiscent of the adsorption of this long, straight-chain molecule on water surface. It is believed that these molecules are hydrogen-bonded to electronegative ions on the apatite surface. Synthetic hydroxyapatite has long been used in chromatographic adsorption columns because of the specific bonding capacity the surfaces have for certain proteins and polynucleotides. The metabolic interrelationship of bone mineral and the body fluids is in great part dependent upon the nature and magnitude of mineral surface. From the surface studies described herein it was suggested that a chemical linkage could exist in bone between the mineral surface and certain free polar groups of collagen.", "contents": "The surface chemistry of bone mineral and related calcium phosphates. A review of the surface chemistry of bone mineral, hydroxyapatite and amorphous calcium phosphate is presented. Small-angle x-ray scattering and low-temperature nitrogen adsorption measurements show the magnitude of bone mineral surface to range from 100-200 m-2/g; the synthetic hydroxyapatite surface can vary from 25-200 m-2/g, while synthetic amorphous calcium phosphate ranges in surface from 20-60 m-2/g, according to the respective preparation conditions. The magnitude of heats of adsorption of certain small molecules (CO, Ar, N2, H2O, CH3OH) on bone mineral and hydroxyapatite show that these are polarizing surfaces that form strong bonds with polar or polarizable molecules; water is hydrogen-bonded to these surfaces with energies ranging from 23 kcal/mole for low coverage to 11 kcal/mole after two full monolayers; concomitantly, methanol ranges from 24 kcal/mole to 9 kcal/mole after the adsorption of one and a half monolayers. Stearic acid will close-pack perpendicularly on bone apatite surfaces when adsorbed from cyclohexane solution in a way reminiscent of the adsorption of this long, straight-chain molecule on water surface. It is believed that these molecules are hydrogen-bonded to electronegative ions on the apatite surface. Synthetic hydroxyapatite has long been used in chromatographic adsorption columns because of the specific bonding capacity the surfaces have for certain proteins and polynucleotides. The metabolic interrelationship of bone mineral and the body fluids is in great part dependent upon the nature and magnitude of mineral surface. From the surface studies described herein it was suggested that a chemical linkage could exist in bone between the mineral surface and certain free polar groups of collagen."}
{"id": "PMID:1091978", "title": "Primary peritonitis in adults.", "content": "Infection within the peritoneal cavity without an identifiable source of contamination is rare. The diagnosis is one of exclusion, and can be made with certainty only after a thorough laparotomy. The response to antibiotic therapy is usually prompt and gratifying.", "contents": "Primary peritonitis in adults. Infection within the peritoneal cavity without an identifiable source of contamination is rare. The diagnosis is one of exclusion, and can be made with certainty only after a thorough laparotomy. The response to antibiotic therapy is usually prompt and gratifying."}
{"id": "PMID:1091979", "title": "Surgical treatment for nonunion of the scaphoid.", "content": "Fracture of the scaphoid is the most common injury of the carpal bones. Most scaphoid fractures heal after immobilization. When the fracture does not unite, degenerative arthritic changes and disability may occur. Twenty-four symptomatic nonunions treated surgically were reviewed. Based on presence of union, percent of normal motion, and patients' symptoms, results were rated good in 19 cases and fair in five. Nine of ten patients treated with bone grafting and styloidectomy had union of fractures with good results. All five patients with bone grafting and internal fixation had union. Excision of proximal fragments of more than one third to one half of the scaphoid, and internal fixation alone, had unsatisfactory results. A styloidectomy should excise approximately one half of the radius articulating with the scaphoid. If not enough styloid is excised, osteophytic overgrowth will occur; if too much is excised, the carpus will sublux radially.", "contents": "Surgical treatment for nonunion of the scaphoid. Fracture of the scaphoid is the most common injury of the carpal bones. Most scaphoid fractures heal after immobilization. When the fracture does not unite, degenerative arthritic changes and disability may occur. Twenty-four symptomatic nonunions treated surgically were reviewed. Based on presence of union, percent of normal motion, and patients' symptoms, results were rated good in 19 cases and fair in five. Nine of ten patients treated with bone grafting and styloidectomy had union of fractures with good results. All five patients with bone grafting and internal fixation had union. Excision of proximal fragments of more than one third to one half of the scaphoid, and internal fixation alone, had unsatisfactory results. A styloidectomy should excise approximately one half of the radius articulating with the scaphoid. If not enough styloid is excised, osteophytic overgrowth will occur; if too much is excised, the carpus will sublux radially."}
{"id": "PMID:1091980", "title": "Thalamotomy for psychopathic behavior.", "content": "Six patients characterized by psychopathic behavior were treated with lesions placed in the center median nucleus of the thalamus. There was marked improvement of the psychopathic behavior in five patients. One patient with unilateral thalamotomy was parqially improved. Four of the six patients have been productive and earn a living. The other two have been partially productive. There were no long-lasting postoperative complications after thalamotomy for psychopathic behavior. The various and hyperirritability, and previously have been collectively identified as the \"hyperresponsive syndrome\". The psychopathic behavior in these condition of the diencephalon.", "contents": "Thalamotomy for psychopathic behavior. Six patients characterized by psychopathic behavior were treated with lesions placed in the center median nucleus of the thalamus. There was marked improvement of the psychopathic behavior in five patients. One patient with unilateral thalamotomy was parqially improved. Four of the six patients have been productive and earn a living. The other two have been partially productive. There were no long-lasting postoperative complications after thalamotomy for psychopathic behavior. The various and hyperirritability, and previously have been collectively identified as the \"hyperresponsive syndrome\". The psychopathic behavior in these condition of the diencephalon."}
{"id": "PMID:1091983", "title": "Liver disease, phlegmonous colitis, and gram-negative sepsis.", "content": "Phlegmonous colitis, regarded as a terminal event in serious liver disease and hepatic coma, can also occur in reversible liver disease and can be the source of gram-negative sepsis. This paper presented such a case. Improved management of serious liver disease and hepatic coma should include consideration of colonic inflammation as another site of infection that must be treated to avoid complications of sepsis or peritonitis. Abdominal pain and loose or diarrheal stools should arouse a suspicion of the presence of phlegmonous colitis, and should be an indication for treating it and preventing sepsis.", "contents": "Liver disease, phlegmonous colitis, and gram-negative sepsis. Phlegmonous colitis, regarded as a terminal event in serious liver disease and hepatic coma, can also occur in reversible liver disease and can be the source of gram-negative sepsis. This paper presented such a case. Improved management of serious liver disease and hepatic coma should include consideration of colonic inflammation as another site of infection that must be treated to avoid complications of sepsis or peritonitis. Abdominal pain and loose or diarrheal stools should arouse a suspicion of the presence of phlegmonous colitis, and should be an indication for treating it and preventing sepsis."}
{"id": "PMID:1091985", "title": "Immunofluorescent study of thyroglobulin during development of embryonic thyroid gland function.", "content": "The synthesis of the protein component of thyroglobulin was studies by the method of immunofluorescence in the thyroid glands of rat, guinea pig, chicken, and calf embryos. Diffuse specific fluorescence of the cytoplasm was observed in the prefollicular stage of thyroid gland differentiation. It was assumed that the specific fluorescence is due to low molecular weight precursors of thyroglobulin. During the formation of the follicles specific fluorescence is found at the apical poles of the cells and later it is concentrated at the cell-colloid boundary and in the follicular cavity. In calf fetuses in the second half of embryonic development specific fluorescence is observed in addition in the cytoplasm of the follicular cells.", "contents": "Immunofluorescent study of thyroglobulin during development of embryonic thyroid gland function. The synthesis of the protein component of thyroglobulin was studies by the method of immunofluorescence in the thyroid glands of rat, guinea pig, chicken, and calf embryos. Diffuse specific fluorescence of the cytoplasm was observed in the prefollicular stage of thyroid gland differentiation. It was assumed that the specific fluorescence is due to low molecular weight precursors of thyroglobulin. During the formation of the follicles specific fluorescence is found at the apical poles of the cells and later it is concentrated at the cell-colloid boundary and in the follicular cavity. In calf fetuses in the second half of embryonic development specific fluorescence is observed in addition in the cytoplasm of the follicular cells."}
{"id": "PMID:1091989", "title": "Differentiation of Klebsiella-Enterobacter-Serratia organisms.", "content": "Differentiation of 625 stains of bacteria which fulfilled the requirements laid down for the definition of the tribe Klebsielleae was carried out using 6 biochemical tests. Five hundred and forty-six strains were identified as members of the genus Klebsiella, 46 of the genus Enterobacter and 33 of the genus Serratia.", "contents": "Differentiation of Klebsiella-Enterobacter-Serratia organisms. Differentiation of 625 stains of bacteria which fulfilled the requirements laid down for the definition of the tribe Klebsielleae was carried out using 6 biochemical tests. Five hundred and forty-six strains were identified as members of the genus Klebsiella, 46 of the genus Enterobacter and 33 of the genus Serratia."}
{"id": "PMID:1091992", "title": "Injuries to the skin of the male external genitalia in Southern Africa.", "content": "The more common injuries to the skin of the male external genital organs are described; some of these mutilations are unique to the African continent. The extent of trauma ranges from small penile skin defects after circumcision to complete avulsion of the skin of the penis and scrotum. The techniques of reconstruction are described.", "contents": "Injuries to the skin of the male external genitalia in Southern Africa. The more common injuries to the skin of the male external genital organs are described; some of these mutilations are unique to the African continent. The extent of trauma ranges from small penile skin defects after circumcision to complete avulsion of the skin of the penis and scrotum. The techniques of reconstruction are described."}
{"id": "PMID:1091993", "title": "The prevention of postoperative deep vein thrombosis.", "content": "Numerous recent reports have appeared dealing with the prevention of postoperative deep vein thrombosis. Simple mechanical measures are inadequate, electrical stimulation has complications, but  the motorised foot mover and pneumatic compression appear effective. Drugs such as coumadin, aspirin and dipyridamole are ineffective, but dextran and sodium pentosan polysulphate appear to have a place. However, if the efficacy and safety of low-dose subcutaneous heparin is statistically confirmed in a multicentre trial, it may lead to the elimination of the morbidity and mortality of postoperative thrombo-embolism.", "contents": "The prevention of postoperative deep vein thrombosis. Numerous recent reports have appeared dealing with the prevention of postoperative deep vein thrombosis. Simple mechanical measures are inadequate, electrical stimulation has complications, but  the motorised foot mover and pneumatic compression appear effective. Drugs such as coumadin, aspirin and dipyridamole are ineffective, but dextran and sodium pentosan polysulphate appear to have a place. However, if the efficacy and safety of low-dose subcutaneous heparin is statistically confirmed in a multicentre trial, it may lead to the elimination of the morbidity and mortality of postoperative thrombo-embolism."}
{"id": "PMID:1091994", "title": "Analgesic studies in osteo-arthritis.", "content": "Two studies on a total of 45 patients with chronic osteoarthritis using fenoprofen calcium are presented. Different methods using only subjective criteria (pain intensity scores and pain relief scores) are applied. Measurable analgesic qualities were demonstrated for fenoprofen, which is seen as a valuable addition to the drug therapy of osteo-arthritis.", "contents": "Analgesic studies in osteo-arthritis. Two studies on a total of 45 patients with chronic osteoarthritis using fenoprofen calcium are presented. Different methods using only subjective criteria (pain intensity scores and pain relief scores) are applied. Measurable analgesic qualities were demonstrated for fenoprofen, which is seen as a valuable addition to the drug therapy of osteo-arthritis."}
{"id": "PMID:1091996", "title": "Candid albicans associated with the gastrointestinal tract of the common pigeon (Columbia livia).", "content": "Candida albicans was cultured from the upper gastrointestinal tract of 44% and from the lower alimentary tract in 6% of 50 common pigeons (Columba livia). Thirty per cent of samples of pigeon droppings collected from different locations were positive for the yeast. The colony forming units for positive samples ranged from 2 times 10-2-2-8times 10-6 per g dry feces. The percentage of antigenic Groups A and B for the strains of C. albicans isolated was approximately the same as for strains isolated from human sources.", "contents": "Candid albicans associated with the gastrointestinal tract of the common pigeon (Columbia livia). Candida albicans was cultured from the upper gastrointestinal tract of 44% and from the lower alimentary tract in 6% of 50 common pigeons (Columba livia). Thirty per cent of samples of pigeon droppings collected from different locations were positive for the yeast. The colony forming units for positive samples ranged from 2 times 10-2-2-8times 10-6 per g dry feces. The percentage of antigenic Groups A and B for the strains of C. albicans isolated was approximately the same as for strains isolated from human sources."}
{"id": "PMID:1091997", "title": "Growth curves of the yeast-like form of Paracocidioides bradiliensis.", "content": "Growth curves of the yeast-like form of 4 strains of Paracoccidioides brasiliensis based on colony counts on GGY agar and on cell counts weree made. Mean generation times wer 17, 13 and 21 h. for 3 of the strains. The kinetics of growth for all strains showed that dead cells were present in approximately the same proportion as living cells in the initial inocula and their number increased through the exponential phase of growth at a similar rate as that of living cells. Viability counts made in 2-week old cultures of all strains were negative. It is suggested that during growth P. brasiliensis continuously diffuses a metabolite which is lethal to the fungus. Strains of Blastomyces dermatitidis and Histoplasma capsulatum were also sensitive to this lethal effects.", "contents": "Growth curves of the yeast-like form of Paracocidioides bradiliensis. Growth curves of the yeast-like form of 4 strains of Paracoccidioides brasiliensis based on colony counts on GGY agar and on cell counts weree made. Mean generation times wer 17, 13 and 21 h. for 3 of the strains. The kinetics of growth for all strains showed that dead cells were present in approximately the same proportion as living cells in the initial inocula and their number increased through the exponential phase of growth at a similar rate as that of living cells. Viability counts made in 2-week old cultures of all strains were negative. It is suggested that during growth P. brasiliensis continuously diffuses a metabolite which is lethal to the fungus. Strains of Blastomyces dermatitidis and Histoplasma capsulatum were also sensitive to this lethal effects."}
{"id": "PMID:1091998", "title": "Isolation of keratinophilic fungi from soil in Pavia, Italy.", "content": "The examination of keratinophilic fungi in 125 soil samples collected in Pavia, Italy revealed the following organism: Microsporum gypseum 23, M. vanbreuseghemii 4, Arthroderma uncinatum 4, Ctenomyces serratus 5, Chrysosporium tropicum 5, unidentified gymnoascacean 1. Arthroderma uncinatum, M. gypseum, M. vanbreuseghemii were isolated from a city park and cultivated soils; C. tropicum, Ct. serratus and an unidentified gymnoascacean were found only in soils from natural woods. The distribution of the different species is discussed.", "contents": "Isolation of keratinophilic fungi from soil in Pavia, Italy. The examination of keratinophilic fungi in 125 soil samples collected in Pavia, Italy revealed the following organism: Microsporum gypseum 23, M. vanbreuseghemii 4, Arthroderma uncinatum 4, Ctenomyces serratus 5, Chrysosporium tropicum 5, unidentified gymnoascacean 1. Arthroderma uncinatum, M. gypseum, M. vanbreuseghemii were isolated from a city park and cultivated soils; C. tropicum, Ct. serratus and an unidentified gymnoascacean were found only in soils from natural woods. The distribution of the different species is discussed."}
{"id": "PMID:1091999", "title": "The action of miconazole of the growth of Candida albicans.", "content": "The growth of Candida albicans was studied in control cultures and in the presence of miconazole or clotrimazole. Each drug prolonged the lag phase and reduced the total final population. Although miconazole, at the low concentrations used, was a less potent inhibitor than clotrimazole in the main logarithmic phase, it was more fungicidal. The antifungal activity of miconazole on C. albicans was inversely proportional to the number of cells inoculated in the media. The effects of miconazole on growth depended on the nutrients in the medium and were most pronouncedwhen it was added to cultures of C. albicans in the lag and main logarithmic phase of growth. The growth inhibitory effects of sub-fungicidal doses of micronazole (smaller than or equal to 10-6 M) on C. albicans seemed to be reversible.", "contents": "The action of miconazole of the growth of Candida albicans. The growth of Candida albicans was studied in control cultures and in the presence of miconazole or clotrimazole. Each drug prolonged the lag phase and reduced the total final population. Although miconazole, at the low concentrations used, was a less potent inhibitor than clotrimazole in the main logarithmic phase, it was more fungicidal. The antifungal activity of miconazole on C. albicans was inversely proportional to the number of cells inoculated in the media. The effects of miconazole on growth depended on the nutrients in the medium and were most pronouncedwhen it was added to cultures of C. albicans in the lag and main logarithmic phase of growth. The growth inhibitory effects of sub-fungicidal doses of micronazole (smaller than or equal to 10-6 M) on C. albicans seemed to be reversible."}
{"id": "PMID:1092000", "title": "A re-evaluation of the effect of cysteine or Candida albicans.", "content": "The effect of cysteine on Candida albicans has been re-examined. Concentrations between 0-1 and 10-0 mM were tested on 12 isolates in yeast and hyphal form. The growth of both forms was inhibited by 10 mM cysteine. Though the rate of hyphal elongation was decreased there was no suppression of initiation of hyphal formation. All isolates showed a random variation in their general inhibitory response to cysteine. We found no support for the concept of cysteine-mediated from determination in rapidly growing C. albicans in submerged culture. High concentrations of cystiene cause a general inhibition of growth of C. albicans.", "contents": "A re-evaluation of the effect of cysteine or Candida albicans. The effect of cysteine on Candida albicans has been re-examined. Concentrations between 0-1 and 10-0 mM were tested on 12 isolates in yeast and hyphal form. The growth of both forms was inhibited by 10 mM cysteine. Though the rate of hyphal elongation was decreased there was no suppression of initiation of hyphal formation. All isolates showed a random variation in their general inhibitory response to cysteine. We found no support for the concept of cysteine-mediated from determination in rapidly growing C. albicans in submerged culture. High concentrations of cystiene cause a general inhibition of growth of C. albicans."}
{"id": "PMID:1092001", "title": "Treatment of accelerated hypertension and end stage renal failure by bilateral nephrectomy and renal transplantation.", "content": "Accelerated hypertension with end stage renal failure not responsive to hemodialysis and ultrafiltration was an indication for bilateral nephrectomy in 22 patients, five of whom required the procedure as an emergency. Normotension occurred in every patient after removal of the kidneys, but hypertension returned in 12 persons after renal transplantation. Five of these required long term antihypertensive medication, and in an additional seven, some form of diuretic was necessary. There were no distinguishing features among those patients in whom post-transplant hypertension developed from those in whom it did not. In spite of severe accelerated hypertension in the patient with well documented chronic renal disease, prompt nephrectomy and renal transplantation were compatible, with an 86 per cent patient survival rate. Seventy-seven per cent of the kidneys functioned for an average of 29 months.", "contents": "Treatment of accelerated hypertension and end stage renal failure by bilateral nephrectomy and renal transplantation. Accelerated hypertension with end stage renal failure not responsive to hemodialysis and ultrafiltration was an indication for bilateral nephrectomy in 22 patients, five of whom required the procedure as an emergency. Normotension occurred in every patient after removal of the kidneys, but hypertension returned in 12 persons after renal transplantation. Five of these required long term antihypertensive medication, and in an additional seven, some form of diuretic was necessary. There were no distinguishing features among those patients in whom post-transplant hypertension developed from those in whom it did not. In spite of severe accelerated hypertension in the patient with well documented chronic renal disease, prompt nephrectomy and renal transplantation were compatible, with an 86 per cent patient survival rate. Seventy-seven per cent of the kidneys functioned for an average of 29 months."}
{"id": "PMID:1092013", "title": "Use of the axillary artery in complex cerebral revascularization.", "content": "Two cases of complex common carotid and innominate artery disease managed by axillocarotid autogenous saphenous vein bypass are presented, including a detailed description of the operative technique. The results in each case were gratifying. Although the concept of extra-anatomic repair is not new, this modification in operative technique expands the surgeon's armamentarium. The ease and safety of exposure and anastomosis of the axillary artery combine to make it the preferable donor vessel for extra-anatomic cerebral revascularization in the poor-risk, elderly patient population.", "contents": "Use of the axillary artery in complex cerebral revascularization. Two cases of complex common carotid and innominate artery disease managed by axillocarotid autogenous saphenous vein bypass are presented, including a detailed description of the operative technique. The results in each case were gratifying. Although the concept of extra-anatomic repair is not new, this modification in operative technique expands the surgeon's armamentarium. The ease and safety of exposure and anastomosis of the axillary artery combine to make it the preferable donor vessel for extra-anatomic cerebral revascularization in the poor-risk, elderly patient population."}
{"id": "PMID:1092014", "title": "The surgical amphitheatre, history of its origins, functions, and fate.", "content": "The Surgical Amphitheatre, patterned after the earlier model of Anatomists, came into being in the 17th century and served the purpose of teaching the anatomy of operation to surgeons, but without hospital connections. A great decline of student and visitor interest in viewing operations attended disappearance of the gambler's risk of operations, followed at the turn of this century by a lesser interest of medical students in attending surgical lectures, a circumstance that provoked consternation in the ranks of surgical teachers. The demise of the surgical amphitheatre as a suitable site for surgical teaching and viewing operations marked the beginnings of a gradual decline of influence of surgery in medical school faculties. Acceptance by reluctant surgeons of the inescapable microbiologic principle that silence of the entire Operating Team is mandatory, when operations are in process, will obviously erode further usefulness of the operating arena for instruction of medical students. Over the past decade, the current sharpened focus upon dispersion of medical care has served to de-emphasize in our medical faculties the post-World War II stress upon research for medicine's advance. A gradual decrease of student interest in surgery has occasioned medical faculties to question the justification of continuing surgery as a major curricular discipline for undergraduate medical students. Surgeons need to cultivate the intimate teacher-student relationship reflected in the practices of Pierre Louis and William Osler. A critical reassessment by professors of surgery of their teaching responsibilities should serve to indicate to medical students and their faculties that surgery has much important and useful information to impart, and that their professors are ready, prepared, and anxious to meet the challenge.", "contents": "The surgical amphitheatre, history of its origins, functions, and fate. The Surgical Amphitheatre, patterned after the earlier model of Anatomists, came into being in the 17th century and served the purpose of teaching the anatomy of operation to surgeons, but without hospital connections. A great decline of student and visitor interest in viewing operations attended disappearance of the gambler's risk of operations, followed at the turn of this century by a lesser interest of medical students in attending surgical lectures, a circumstance that provoked consternation in the ranks of surgical teachers. The demise of the surgical amphitheatre as a suitable site for surgical teaching and viewing operations marked the beginnings of a gradual decline of influence of surgery in medical school faculties. Acceptance by reluctant surgeons of the inescapable microbiologic principle that silence of the entire Operating Team is mandatory, when operations are in process, will obviously erode further usefulness of the operating arena for instruction of medical students. Over the past decade, the current sharpened focus upon dispersion of medical care has served to de-emphasize in our medical faculties the post-World War II stress upon research for medicine's advance. A gradual decrease of student interest in surgery has occasioned medical faculties to question the justification of continuing surgery as a major curricular discipline for undergraduate medical students. Surgeons need to cultivate the intimate teacher-student relationship reflected in the practices of Pierre Louis and William Osler. A critical reassessment by professors of surgery of their teaching responsibilities should serve to indicate to medical students and their faculties that surgery has much important and useful information to impart, and that their professors are ready, prepared, and anxious to meet the challenge."}
{"id": "PMID:1092015", "title": "Experience with lymphoceles after renal transplantation.", "content": "Of 215 patients who underwent renal transplantations at the Mayo Clinic, six (2.8 percent) developed perirenal lymphoceles. Diagnosis was made between 5 weeks and 14 months after operation. In all six patients, renal function decreased and eventually a lower quadrant abdominal mass developed. Ipsilateral leg edema, fluid retention, and development of diastolic hypertension should also increase suspicion as to the possibility of lymph collection in the perirenal space. Excretory urography with tomography delineated the lymphocele in each instance; B scan ultrasound may be an additional valuable diagnostic tool. Drainage procedures readily restored normal renal function and, in one instance, drainage also restored the blood pressure to normal. Percutaneous needle aspiration seems to be a temporary measure only, and marsupialization is the preferred definite procedure.", "contents": "Experience with lymphoceles after renal transplantation. Of 215 patients who underwent renal transplantations at the Mayo Clinic, six (2.8 percent) developed perirenal lymphoceles. Diagnosis was made between 5 weeks and 14 months after operation. In all six patients, renal function decreased and eventually a lower quadrant abdominal mass developed. Ipsilateral leg edema, fluid retention, and development of diastolic hypertension should also increase suspicion as to the possibility of lymph collection in the perirenal space. Excretory urography with tomography delineated the lymphocele in each instance; B scan ultrasound may be an additional valuable diagnostic tool. Drainage procedures readily restored normal renal function and, in one instance, drainage also restored the blood pressure to normal. Percutaneous needle aspiration seems to be a temporary measure only, and marsupialization is the preferred definite procedure."}
{"id": "PMID:1092016", "title": "Analysis of the optimal perfusion pressure and flow rate of the renal vascular resistance and oxygen consumption in the hypothermic perfused kidney.", "content": "Thirty-six dog kidneys were perfused with different perfusion pressures (between 15 and 60 mm. Hg) for 72 hours and then transplanted. Hypothermic human albumin was the perfusion fluid.  Enzyme release, kidney weight, and renal oxygen consumption were measured during perfusion. Kidneys perfused with a flow rate of 0.8 ml. per gram per minute (21 mm. Hg mean perfusion pressure) showed the smallest increase in kidney weight and the best function after transplantation. Renal vascular resistance was independent of the level of the perfusion pressure and renal oxygen consumption was independent of the applied flow rate. It is concluded that the perfusion pressure applied with hypothermic perfusion should be as low as possible because in this way kidney damage caused by perfusion can be avoided most easily.", "contents": "Analysis of the optimal perfusion pressure and flow rate of the renal vascular resistance and oxygen consumption in the hypothermic perfused kidney. Thirty-six dog kidneys were perfused with different perfusion pressures (between 15 and 60 mm. Hg) for 72 hours and then transplanted. Hypothermic human albumin was the perfusion fluid.  Enzyme release, kidney weight, and renal oxygen consumption were measured during perfusion. Kidneys perfused with a flow rate of 0.8 ml. per gram per minute (21 mm. Hg mean perfusion pressure) showed the smallest increase in kidney weight and the best function after transplantation. Renal vascular resistance was independent of the level of the perfusion pressure and renal oxygen consumption was independent of the applied flow rate. It is concluded that the perfusion pressure applied with hypothermic perfusion should be as low as possible because in this way kidney damage caused by perfusion can be avoided most easily."}
{"id": "PMID:1092019", "title": "An improved nonsuture method for portacaval anastomosis in the rat.", "content": "A nonsuture technique is described for performing portacaval anastomoses in rats. Improvements include reduced clamping time on critical vessels, reduced blood loss, uniformly patent anastomoses, easily manufactured buttons, and decreased mortality rates. The new procedure allows a single operator to perform 25 such operations per day, thus substantially increasing the availability of these animals for studies in hepatic metabolism following portacaval anastomoses.", "contents": "An improved nonsuture method for portacaval anastomosis in the rat. A nonsuture technique is described for performing portacaval anastomoses in rats. Improvements include reduced clamping time on critical vessels, reduced blood loss, uniformly patent anastomoses, easily manufactured buttons, and decreased mortality rates. The new procedure allows a single operator to perform 25 such operations per day, thus substantially increasing the availability of these animals for studies in hepatic metabolism following portacaval anastomoses."}
{"id": "PMID:1092022", "title": "Experience with a modification of Duhamel-Grob-Martin operation for the treatment of Hirschsprung's disease.", "content": "Forty-two patients have been operated upon with the Duhamel-Grob-Martin operation to which has been added a partial sphincterotomy. Three patients developed anastomotic leaks and one of these died. In follow-up studies from 2 months to 9 years after operation, all patients were relieved of distension and constipation, except two, who were considered to have had an incomplete proximal resection of aganglionic bowel. There were two children with repeated attacks of enterocolitis after operation. Fecal control was good in all and, in spite of the retention of a considerable spur, there have been no problems from this. It is considered that the internal sphincter is involved in the disease and the possibility of persistent obstructive symptoms is reduced by the internal sphincterotomy which has not resulted in incontinence.", "contents": "Experience with a modification of Duhamel-Grob-Martin operation for the treatment of Hirschsprung's disease. Forty-two patients have been operated upon with the Duhamel-Grob-Martin operation to which has been added a partial sphincterotomy. Three patients developed anastomotic leaks and one of these died. In follow-up studies from 2 months to 9 years after operation, all patients were relieved of distension and constipation, except two, who were considered to have had an incomplete proximal resection of aganglionic bowel. There were two children with repeated attacks of enterocolitis after operation. Fecal control was good in all and, in spite of the retention of a considerable spur, there have been no problems from this. It is considered that the internal sphincter is involved in the disease and the possibility of persistent obstructive symptoms is reduced by the internal sphincterotomy which has not resulted in incontinence."}
{"id": "PMID:1092023", "title": "Adjustable razor dermatome: another efficient, economical, and portable dermatome.", "content": "The adjustable razor dermatome is another simple, portable, inexpensive, yet efficient mechanical tool for skin grafting. The dermatome is constructed easily from an ordinary household adjustable safety razor. This model, unlike those described earlier will cut grafts of various thicknesses without the use of a different cutting head or shim.", "contents": "Adjustable razor dermatome: another efficient, economical, and portable dermatome. The adjustable razor dermatome is another simple, portable, inexpensive, yet efficient mechanical tool for skin grafting. The dermatome is constructed easily from an ordinary household adjustable safety razor. This model, unlike those described earlier will cut grafts of various thicknesses without the use of a different cutting head or shim."}
{"id": "PMID:1092032", "title": "The metabolism of saccharin in animals.", "content": "Rats given oral doses of [3-14C] saccharin excreted 56-87% of the labeled dose in the urine and 10-40% in the feces during 7 days. Distribution studies showed that the highest levels of 14C were in the kidneys and bladders. The bile was not a significant route of excretion. The presence of labeled CO2 in expired air indicated that saccharin was decarbosylated to a slight degree. DEAE chromatographic separation and isolation of labeled compounds from urine samples showed that more than 99% of the urinary 14C was unchanged saccharin. Up to 1.0% of the 14C was a metabolite identified as 0-sulfamoylbenzoic acid. Comparative metabolic profiles of a dog, rabbit, guinea pig and hamster indicated that there was little difference in the pattern due to animal species or dose level.", "contents": "The metabolism of saccharin in animals. Rats given oral doses of [3-14C] saccharin excreted 56-87% of the labeled dose in the urine and 10-40% in the feces during 7 days. Distribution studies showed that the highest levels of 14C were in the kidneys and bladders. The bile was not a significant route of excretion. The presence of labeled CO2 in expired air indicated that saccharin was decarbosylated to a slight degree. DEAE chromatographic separation and isolation of labeled compounds from urine samples showed that more than 99% of the urinary 14C was unchanged saccharin. Up to 1.0% of the 14C was a metabolite identified as 0-sulfamoylbenzoic acid. Comparative metabolic profiles of a dog, rabbit, guinea pig and hamster indicated that there was little difference in the pattern due to animal species or dose level."}
{"id": "PMID:1092033", "title": "A comparison of betahistine hydrochloride with placebo for vertebral-basilar insufficiency: a double-blind study.", "content": "To test the effectiveness of betahistine HC1 in reducing the frequency of transient ischemic attacks (TIAs) caused by vertebral-basilar artery insufficiency, we randomly assigned 26 patients with a typical history of the condition to a placebo-drug or a drug-placebo sequence, each sequence lasting two months. During the study, the frequency of TIAs did not differ significantly between the placebo and the drug groups. Subjective responses indicated some value for betahistine as a palliative agent.", "contents": "A comparison of betahistine hydrochloride with placebo for vertebral-basilar insufficiency: a double-blind study. To test the effectiveness of betahistine HC1 in reducing the frequency of transient ischemic attacks (TIAs) caused by vertebral-basilar artery insufficiency, we randomly assigned 26 patients with a typical history of the condition to a placebo-drug or a drug-placebo sequence, each sequence lasting two months. During the study, the frequency of TIAs did not differ significantly between the placebo and the drug groups. Subjective responses indicated some value for betahistine as a palliative agent."}
{"id": "PMID:1092064", "title": "Erythrocyte polyagglutination showing properties of both T and Tk, probably induced by Bacteroides fragilis infection.", "content": "A new type of polyagglutination is described in which the T and Tk receptors are both exposed on the erythrocyte membrane. It is suggested that there may be an association between this type of polyagglutination and infection by Bacteroides fragilis.", "contents": "Erythrocyte polyagglutination showing properties of both T and Tk, probably induced by Bacteroides fragilis infection. A new type of polyagglutination is described in which the T and Tk receptors are both exposed on the erythrocyte membrane. It is suggested that there may be an association between this type of polyagglutination and infection by Bacteroides fragilis."}
{"id": "PMID:1092065", "title": "Qualitative and quantitative study of the antigens and antibodies of the HL-A system-automatic fluorochrome method.", "content": "It was possible to obtain a successful evaluation of the quantity of HL-A antigens on lymphocytes using a fluorochromatic test with double marking by fluorescine diacetate and ethidium bromide. It was also possible to detect anti-HL-A antibodies, to study their cross-reactions and to perform tissue typing using this automatic, sensitive and repeatable method.", "contents": "Qualitative and quantitative study of the antigens and antibodies of the HL-A system-automatic fluorochrome method. It was possible to obtain a successful evaluation of the quantity of HL-A antigens on lymphocytes using a fluorochromatic test with double marking by fluorescine diacetate and ethidium bromide. It was also possible to detect anti-HL-A antibodies, to study their cross-reactions and to perform tissue typing using this automatic, sensitive and repeatable method."}
{"id": "PMID:1092072", "title": "[Method for preparing monospecific antiserum to fowl plague virus hemagglutinins].", "content": "A simple method for preparation of monospecific antiserum for the hemagglutinin of fowl plague virus has been developed. It is based on selective inactivation of the enzymatic and antigenic properties of neuraminidase by heating of the virus at 56degrees for 3 hours followed by disruption of the preparation with detergent and removal of the inner proteins by ultracentrifugation. Immunization of animals with such preparations produced antiserum containing considerable amounts of antibody for the hemagglutinin in the absence of antibody for other proteins of fowl plague virus.", "contents": "[Method for preparing monospecific antiserum to fowl plague virus hemagglutinins]. A simple method for preparation of monospecific antiserum for the hemagglutinin of fowl plague virus has been developed. It is based on selective inactivation of the enzymatic and antigenic properties of neuraminidase by heating of the virus at 56degrees for 3 hours followed by disruption of the preparation with detergent and removal of the inner proteins by ultracentrifugation. Immunization of animals with such preparations produced antiserum containing considerable amounts of antibody for the hemagglutinin in the absence of antibody for other proteins of fowl plague virus."}
{"id": "PMID:1092073", "title": "[Fibrin removal from mouse immune ascitic fluids to arbovirus using beta-propiolactone].", "content": "A modification of acid precipitation of fibrin in immune ascitic fluids (IAF) employing beta-propiolactone has been developed. The procedure removes fibrin completely without affecting the titres of specific antibody. This modification is not inferior to previously described methods for fibrin removal from IAF and has a number of advantages. In addition to removing fibrin from IAF, beta-propiolactone prevents possible virus contamination of IAF and sterilizes the preparation additionally.", "contents": "[Fibrin removal from mouse immune ascitic fluids to arbovirus using beta-propiolactone]. A modification of acid precipitation of fibrin in immune ascitic fluids (IAF) employing beta-propiolactone has been developed. The procedure removes fibrin completely without affecting the titres of specific antibody. This modification is not inferior to previously described methods for fibrin removal from IAF and has a number of advantages. In addition to removing fibrin from IAF, beta-propiolactone prevents possible virus contamination of IAF and sterilizes the preparation additionally."}
{"id": "PMID:1092074", "title": "[Clinical and epidemiological aspects of influenza chemoprophylaxis with bonaphthone].", "content": "The results of double blind clinical, phisiological, biochemical immunological and virological studies carried out in 460 normal volunteers gave sufficiently objective evaluation of safety and antiinfluenza activity of bonaphthone. The new antiviral drug is recommended for wide epidemiological trials. In epidemiological, coded and strictly controlled experiment during influenza A/England epidemic in 1973 involving 1393 persons the prophylactic effectiveness of bonaphthone was established (the index of effectiveness 2.09).", "contents": "[Clinical and epidemiological aspects of influenza chemoprophylaxis with bonaphthone]. The results of double blind clinical, phisiological, biochemical immunological and virological studies carried out in 460 normal volunteers gave sufficiently objective evaluation of safety and antiinfluenza activity of bonaphthone. The new antiviral drug is recommended for wide epidemiological trials. In epidemiological, coded and strictly controlled experiment during influenza A/England epidemic in 1973 involving 1393 persons the prophylactic effectiveness of bonaphthone was established (the index of effectiveness 2.09)."}
{"id": "PMID:1092075", "title": "[Biological and physicochemical properties of virus PBV-1, isolated from Penicillium brevi compactum].", "content": "High infectivity of PBV-1 virus for different strains of E. coli has been demonstrated. Sizes of the virus particle are; head diameter 513 angstrom, length of the process 1500 angstrom, diameter of the process 60 angstrom. The buoyant density of the virus in cesium chloride density gradient is 1.49 g/cm3, in cesium sulphate-1.39 g/cm3, the sedimentation constant of the virus particle is 380S; molecular weight is 52.8 plus or minus 1.9 times 10-6 daltons. The PBV-1 virus contains double-stranded DNA with the melting temperature in 1 SSC (0.15 M NaCl plus 0.0015 M sodium citrate, pH 7.0) and in 0.1 SSC-88.5 degrees C, respectively. The buoyant density of DNA in cesium chloride gradient is 1.7058 g/cm3 and in cesium sulphate 1.424 g/cm3. The content of GC-pairs calculated by the melting temperature is 46.5% and by the buoyant density 46.7%. On the basis of DNA sedimentation constant value (30S) the average molecular weight of DNA has been calculated to be 23.7 times 10-6 daltons.", "contents": "[Biological and physicochemical properties of virus PBV-1, isolated from Penicillium brevi compactum]. High infectivity of PBV-1 virus for different strains of E. coli has been demonstrated. Sizes of the virus particle are; head diameter 513 angstrom, length of the process 1500 angstrom, diameter of the process 60 angstrom. The buoyant density of the virus in cesium chloride density gradient is 1.49 g/cm3, in cesium sulphate-1.39 g/cm3, the sedimentation constant of the virus particle is 380S; molecular weight is 52.8 plus or minus 1.9 times 10-6 daltons. The PBV-1 virus contains double-stranded DNA with the melting temperature in 1 SSC (0.15 M NaCl plus 0.0015 M sodium citrate, pH 7.0) and in 0.1 SSC-88.5 degrees C, respectively. The buoyant density of DNA in cesium chloride gradient is 1.7058 g/cm3 and in cesium sulphate 1.424 g/cm3. The content of GC-pairs calculated by the melting temperature is 46.5% and by the buoyant density 46.7%. On the basis of DNA sedimentation constant value (30S) the average molecular weight of DNA has been calculated to be 23.7 times 10-6 daltons."}
{"id": "PMID:1092076", "title": "[Hemagglutination tests with insect viruses].", "content": "Nucleopolyhedrosis viruses isolated from larvae of Mamestra brassicae L. and larvae of Ocneria dispar L. as well as granulosis virus isolated from Apamea anceps Shiff. were found to agglutinate red blood cells from a variety of animal species. Chicken and goose erythrocytes proved to be most sensitive. The haemagglutination-inhibition test with the virus of Mamestra brassicae and with specific mouse immune ascitic fluid (IAF) was positive. The titer of IAF was 1:160.", "contents": "[Hemagglutination tests with insect viruses]. Nucleopolyhedrosis viruses isolated from larvae of Mamestra brassicae L. and larvae of Ocneria dispar L. as well as granulosis virus isolated from Apamea anceps Shiff. were found to agglutinate red blood cells from a variety of animal species. Chicken and goose erythrocytes proved to be most sensitive. The haemagglutination-inhibition test with the virus of Mamestra brassicae and with specific mouse immune ascitic fluid (IAF) was positive. The titer of IAF was 1:160."}
{"id": "PMID:1092090", "title": "[Sensitivity of different strains of E. coli and Shigella to the inhibitory action of Ploskirev's bactoagar].", "content": "A study was made of the sensitivity of 190 strains of E. coli and 107 strains of Shigellae of different serological types to the inhibitory action of Ploskirev's bactoagar on the capacity of these bacteria to form colonies. The strains under study proved to be nonhomogenous in this respect: between different strains of E. coli there were revealed 10-, 100- and even 1000-fold differences by this property; the differences between Shigellae strains were less marked--in over 90% of Shigellae strains the capacity to form colonies was depressed less than 4-fold. Only with the aid of some of E. coli and Shigellae strains was it possible to detect differences between the tested 20 commercial Ploskirev's bactoagar batches. Apparently, the use in practical bacteriological laboratories of accidental strains of local origin for control of Ploskirev's bactoagar could not aid in improving the quality of the medium, and consequently bacteriological diagnosis of infections.", "contents": "[Sensitivity of different strains of E. coli and Shigella to the inhibitory action of Ploskirev's bactoagar]. A study was made of the sensitivity of 190 strains of E. coli and 107 strains of Shigellae of different serological types to the inhibitory action of Ploskirev's bactoagar on the capacity of these bacteria to form colonies. The strains under study proved to be nonhomogenous in this respect: between different strains of E. coli there were revealed 10-, 100- and even 1000-fold differences by this property; the differences between Shigellae strains were less marked--in over 90% of Shigellae strains the capacity to form colonies was depressed less than 4-fold. Only with the aid of some of E. coli and Shigellae strains was it possible to detect differences between the tested 20 commercial Ploskirev's bactoagar batches. Apparently, the use in practical bacteriological laboratories of accidental strains of local origin for control of Ploskirev's bactoagar could not aid in improving the quality of the medium, and consequently bacteriological diagnosis of infections."}
{"id": "PMID:1092091", "title": "[Lytic antibodies in antibacterial sera. IV. Lytic activity of H-antibodies].", "content": "The authors studied the lytic and agglutinating activity of H-antibodies against S. typhimurium. Sera containing H-antibodies proved to possess a distinct lytic activity expressed in the presence of a complement and caused by specific antibodies. In H-antibodies specific lytic activity (as per 1 agglutinating unit) was much weaker than in O-antibodies. A relatively low lytic action of H-antibodies was apparently associated with topography of corresponding antigenic determinants of the microbial cell which determined the low possibility of fatal injuries. In examination of H-bacteriolysins there was revealed the same regularity as noted in studying the O-bacteriolysins: reduction of the lytic activity of antibodies in hyperimmunization.", "contents": "[Lytic antibodies in antibacterial sera. IV. Lytic activity of H-antibodies]. The authors studied the lytic and agglutinating activity of H-antibodies against S. typhimurium. Sera containing H-antibodies proved to possess a distinct lytic activity expressed in the presence of a complement and caused by specific antibodies. In H-antibodies specific lytic activity (as per 1 agglutinating unit) was much weaker than in O-antibodies. A relatively low lytic action of H-antibodies was apparently associated with topography of corresponding antigenic determinants of the microbial cell which determined the low possibility of fatal injuries. In examination of H-bacteriolysins there was revealed the same regularity as noted in studying the O-bacteriolysins: reduction of the lytic activity of antibodies in hyperimmunization."}
{"id": "PMID:1092092", "title": "[Regression analysis of the relation between antitoxic activity of sera (Jensen method) and antibody titers in the passive hemagglutination reaction].", "content": "Antidiphtherial and antitetanus antitoxic immunity was determined in the sera of 1588 children (20 experiments) and 611 children and adults (12 experiments) by two methods--on animals and in the passive hemagglutination test (PHT). Factual data were grouped in accordance with a conditioned scale of the antitoxin values in IU composed of the values average for each interval. The series of two-fold dilutions represented a scale of titres of the same sera in the PHT. Mathematical analysis showed that there was a direct linear regressive relationship between the indices of antitoxic immunity obtained by different methods. As a rule, regression coefficients were below a unit, i. e. the rate of increase of the values by the HDT titre scale was somewhat retarded from the growth by the IU titre, this pointing to the difference in their value. The free member of the regression equation (a) characterizing this choice of the sera varied widely. Between the regression coefficients calculated for individual experiments statistically significant differences were revealed in almost 50% of cases. Transformation of quantitative indices of one test into quantitative characteristics obtained by the other method, with the aid of regression equation calculated outside the definite experiment, proved to be unfounded.", "contents": "[Regression analysis of the relation between antitoxic activity of sera (Jensen method) and antibody titers in the passive hemagglutination reaction]. Antidiphtherial and antitetanus antitoxic immunity was determined in the sera of 1588 children (20 experiments) and 611 children and adults (12 experiments) by two methods--on animals and in the passive hemagglutination test (PHT). Factual data were grouped in accordance with a conditioned scale of the antitoxin values in IU composed of the values average for each interval. The series of two-fold dilutions represented a scale of titres of the same sera in the PHT. Mathematical analysis showed that there was a direct linear regressive relationship between the indices of antitoxic immunity obtained by different methods. As a rule, regression coefficients were below a unit, i. e. the rate of increase of the values by the HDT titre scale was somewhat retarded from the growth by the IU titre, this pointing to the difference in their value. The free member of the regression equation (a) characterizing this choice of the sera varied widely. Between the regression coefficients calculated for individual experiments statistically significant differences were revealed in almost 50% of cases. Transformation of quantitative indices of one test into quantitative characteristics obtained by the other method, with the aid of regression equation calculated outside the definite experiment, proved to be unfounded."}
{"id": "PMID:1092094", "title": "[Genetic control of the ability of Sh. flexneri to produce a lethal effect on macrophages. I. Study of the possible extrachromosomal nature of the cyt+-determinant].", "content": "It was shown that cyt+-determinant could not be eliminated when the Sh. flexneri cultures were treated with ethidium bromide under the regimen providing the elimination of the F'lac-factor in a part of shigellae population. The cytotoxic Sh. flexneri strains which acquired the R-factor transmitted the multiple drug resistance to the cyt minus -strain of Sh. flexneri, but not the cyt+-properties. There was found no correlation between the cytoxicity of the Sh. flexneri strains and their capacity to mobilize the nontransmissive STr-ex-episome in the triple cross experiments. The results obtained were in favour of the chromosomal nature of cyt+-determinants controlling the capacity of Sh. flexneri to produce the lethal action on the macrophages.", "contents": "[Genetic control of the ability of Sh. flexneri to produce a lethal effect on macrophages. I. Study of the possible extrachromosomal nature of the cyt+-determinant]. It was shown that cyt+-determinant could not be eliminated when the Sh. flexneri cultures were treated with ethidium bromide under the regimen providing the elimination of the F'lac-factor in a part of shigellae population. The cytotoxic Sh. flexneri strains which acquired the R-factor transmitted the multiple drug resistance to the cyt minus -strain of Sh. flexneri, but not the cyt+-properties. There was found no correlation between the cytoxicity of the Sh. flexneri strains and their capacity to mobilize the nontransmissive STr-ex-episome in the triple cross experiments. The results obtained were in favour of the chromosomal nature of cyt+-determinants controlling the capacity of Sh. flexneri to produce the lethal action on the macrophages."}
{"id": "PMID:1092096", "title": "[Typing properties of bacteriophages isolated from Sh. sonnei strains].", "content": "On the basis of studying the lytic activity of eight bacteriophages the authors elaborated a scheme of bacteriophage typing of Sh. sonnei consisting of nine bacteriophage types with two subtypes. In typing 2348 strains isolated in various cities of the USSR bacteriophage pattern of Sh. sonnei was found to differ in different towns of the USSR. All the strains isolated repeatedly from the same patients were referred to the same bacteriophage type, this pointing to the stability  of the type-determining properties of bacteriophages. Stability of bacteriophage types, a possibility of typing the strains of the I, II phases and of R-forms, simplicity and distinctness of the scheme of bacteriophage typing determined future prospects in using the type bacteriophages selected at the Tbilisi Institute of Vaccine and Sera for intraspecies differentiation of Sh. sonnei.", "contents": "[Typing properties of bacteriophages isolated from Sh. sonnei strains]. On the basis of studying the lytic activity of eight bacteriophages the authors elaborated a scheme of bacteriophage typing of Sh. sonnei consisting of nine bacteriophage types with two subtypes. In typing 2348 strains isolated in various cities of the USSR bacteriophage pattern of Sh. sonnei was found to differ in different towns of the USSR. All the strains isolated repeatedly from the same patients were referred to the same bacteriophage type, this pointing to the stability  of the type-determining properties of bacteriophages. Stability of bacteriophage types, a possibility of typing the strains of the I, II phases and of R-forms, simplicity and distinctness of the scheme of bacteriophage typing determined future prospects in using the type bacteriophages selected at the Tbilisi Institute of Vaccine and Sera for intraspecies differentiation of Sh. sonnei."}
{"id": "PMID:1092098", "title": "[Polyamines in species of microorganisms of the intestinal group].", "content": "A study was made of polyamines (putrescine, spermidine and spermin) in various representatives of the Enterobacteriaceae family--E. coli, Sh, sonnei, Sh. flexneri and S. typhi abdominalis. All the strains under study contained putrescine and spermidine, and many Shigella and Salmonella strains had spermin in addition. There were significant differences in the quantitative content of polyamines in the individual species. By the ratio of nitrogen of polyamine to phosphorus of nucleic acid it is possible to assess the correlation in the content of polyamines and nucleic acids in the bacterial cells.", "contents": "[Polyamines in species of microorganisms of the intestinal group]. A study was made of polyamines (putrescine, spermidine and spermin) in various representatives of the Enterobacteriaceae family--E. coli, Sh, sonnei, Sh. flexneri and S. typhi abdominalis. All the strains under study contained putrescine and spermidine, and many Shigella and Salmonella strains had spermin in addition. There were significant differences in the quantitative content of polyamines in the individual species. By the ratio of nitrogen of polyamine to phosphorus of nucleic acid it is possible to assess the correlation in the content of polyamines and nucleic acids in the bacterial cells."}
{"id": "PMID:1092101", "title": "[Antibodies to erythrogenic toxin B in scarlatina patients].", "content": "Coupled sera of 240 children suffering from scarlet fever obtained on the 1st-5th and 12th-15th days of the disease were examined in the PHA test with the A and B toxins. Antibodies to toxin B were revealed in 63-69% and to toxin A in 60-66% of the sera. In 64.5% of the cases the sera under study reacted simultaneously with both types of the toxin; antibodies to toxin B were contained in higher titres than to toxin A in 1/3 of the sera. The highest antibody titres to toxin B were revealed during summer.", "contents": "[Antibodies to erythrogenic toxin B in scarlatina patients]. Coupled sera of 240 children suffering from scarlet fever obtained on the 1st-5th and 12th-15th days of the disease were examined in the PHA test with the A and B toxins. Antibodies to toxin B were revealed in 63-69% and to toxin A in 60-66% of the sera. In 64.5% of the cases the sera under study reacted simultaneously with both types of the toxin; antibodies to toxin B were contained in higher titres than to toxin A in 1/3 of the sera. The highest antibody titres to toxin B were revealed during summer."}
{"id": "PMID:1092102", "title": "[An experimental study of auto-allergy evoked by staphylococcus under conditions of primary and secondary immunological response].", "content": "Experiments were conducted on rabbits and albino mice; it was shown that the capacity of the animal organism to reject the skin autotransplant formed 7 to 10 days after the primary immunization and persisted for a period of 45 days. Repeated vaccination of mice 24 hours before the operation aided the take of the skin autotransplants, and 7 days before--promoted their rejection. Intravenous revaccination of the operated animals also intensified the rejection reactions; the process of the transplant rejection was accompanied by a marked fall in the level of staphylococcus- and autoantibodies. In the unoperated animals intravenous revaccination with staphylovaccine caused production of antibodies by the secondary type. Deposits of fixed gamma-globulin were found on the 6th-8th day after the transplantation in the autotransplant and its bed in mice primarily immunized with staphylovaccine. Marked circulatory disturbances, necrobiotic processes, mononuclear infiltration of the derma with neutrophils were revealed pathomorphologically in the rejecting skin transplant.", "contents": "[An experimental study of auto-allergy evoked by staphylococcus under conditions of primary and secondary immunological response]. Experiments were conducted on rabbits and albino mice; it was shown that the capacity of the animal organism to reject the skin autotransplant formed 7 to 10 days after the primary immunization and persisted for a period of 45 days. Repeated vaccination of mice 24 hours before the operation aided the take of the skin autotransplants, and 7 days before--promoted their rejection. Intravenous revaccination of the operated animals also intensified the rejection reactions; the process of the transplant rejection was accompanied by a marked fall in the level of staphylococcus- and autoantibodies. In the unoperated animals intravenous revaccination with staphylovaccine caused production of antibodies by the secondary type. Deposits of fixed gamma-globulin were found on the 6th-8th day after the transplantation in the autotransplant and its bed in mice primarily immunized with staphylovaccine. Marked circulatory disturbances, necrobiotic processes, mononuclear infiltration of the derma with neutrophils were revealed pathomorphologically in the rejecting skin transplant."}
{"id": "PMID:1092103", "title": "[Palatine tonsils and immunity. III. T- and B-lymphocytes in the palatine tonsils of patients with chronic tonsillitis].", "content": "Both B- and T-cells are contained in the palatine tonsils of patients suffering from chronic tonsillitis. The former respond by blast-transformation to the in vitro stimulation by the streptococcus and staphylococcus antigens and the latter--to PHA, and produce soluble substances inducing blast-transformation reaction of lymphoid cells of the palatine tonsils under the effect of streptococcus and staphylococcus antigens.", "contents": "[Palatine tonsils and immunity. III. T- and B-lymphocytes in the palatine tonsils of patients with chronic tonsillitis]. Both B- and T-cells are contained in the palatine tonsils of patients suffering from chronic tonsillitis. The former respond by blast-transformation to the in vitro stimulation by the streptococcus and staphylococcus antigens and the latter--to PHA, and produce soluble substances inducing blast-transformation reaction of lymphoid cells of the palatine tonsils under the effect of streptococcus and staphylococcus antigens."}
{"id": "PMID:1092106", "title": "[The ultrastructure of L-forms. III. The L-forms of S. typhi and Pr. vulgaris].", "content": "A marked polymorphism was revealed in the colonies of 5--7 day L-forms of S. typhi and Pr. vulgaris: among different types of the cells there prevailed spherical or oval forms with a distinct nucleoid. Three-layer cytoplasmic membranes--the site of formazan TNBT deposition in the reaction for succinic dehydrogenase--were detected on the greater part of the cell surface. Sometimes there were revealed on the surface of the membrane residues of the cell wall in the form of structureless material of low electron density or pieces of external membrane. Myelin-like structures, mesosomes of ring or lamellar structure and two-contour vesicles located directly in the cytoplasm were found in the cytoplasm. L-form cells displayed binary fission or reproduced by budding; in the latter case there formed elementary bodies surrounded by a three-layer membrane, not infrequently with a visible nucleoid and structures resembling mesosomes of the lamellar type.", "contents": "[The ultrastructure of L-forms. III. The L-forms of S. typhi and Pr. vulgaris]. A marked polymorphism was revealed in the colonies of 5--7 day L-forms of S. typhi and Pr. vulgaris: among different types of the cells there prevailed spherical or oval forms with a distinct nucleoid. Three-layer cytoplasmic membranes--the site of formazan TNBT deposition in the reaction for succinic dehydrogenase--were detected on the greater part of the cell surface. Sometimes there were revealed on the surface of the membrane residues of the cell wall in the form of structureless material of low electron density or pieces of external membrane. Myelin-like structures, mesosomes of ring or lamellar structure and two-contour vesicles located directly in the cytoplasm were found in the cytoplasm. L-form cells displayed binary fission or reproduced by budding; in the latter case there formed elementary bodies surrounded by a three-layer membrane, not infrequently with a visible nucleoid and structures resembling mesosomes of the lamellar type."}
{"id": "PMID:1092107", "title": "[A comparative immunochemical study of the subtypes of serologic type I of the agent of pseudotuberculosis].", "content": "It was shown that Y. pseudotuberculosis strains causing the Far-Eastern scarlatina-like fever in the Primorsk region belonged to subtype IB-ipopolysaccharides of the standard strain of subtype IB and of the local strain were closely affiliated by the analytic data and monosaccharide composition, but differed from the lipopolysaccharide of strains belonging to subtype IA. Living vaccines should be used to obtain the sera against the subtypes IA and IB of the causative agent.", "contents": "[A comparative immunochemical study of the subtypes of serologic type I of the agent of pseudotuberculosis]. It was shown that Y. pseudotuberculosis strains causing the Far-Eastern scarlatina-like fever in the Primorsk region belonged to subtype IB-ipopolysaccharides of the standard strain of subtype IB and of the local strain were closely affiliated by the analytic data and monosaccharide composition, but differed from the lipopolysaccharide of strains belonging to subtype IA. Living vaccines should be used to obtain the sera against the subtypes IA and IB of the causative agent."}
{"id": "PMID:1092108", "title": "[The role of the ability of Sh. flexneri to synthesize pili of the common type in the development of dysenteric infections].", "content": "HEp-2 epithelial cells infected with isogenic Sh. flexneri strains characterized by capacity to synthesize common pili (confirmed by electron microscopy) were studied. It was shown that although pili promoted an earlier adhesion of shigellae to the cells, they were not very significant for the penetration and intracellular development of dysentery bacilli in the epithelium. The use of bacteria labeled with 3H-glucose offers a possibility of an objective rapid and precise determination of the capacity of the microbes to adhere to the cell surface.", "contents": "[The role of the ability of Sh. flexneri to synthesize pili of the common type in the development of dysenteric infections]. HEp-2 epithelial cells infected with isogenic Sh. flexneri strains characterized by capacity to synthesize common pili (confirmed by electron microscopy) were studied. It was shown that although pili promoted an earlier adhesion of shigellae to the cells, they were not very significant for the penetration and intracellular development of dysentery bacilli in the epithelium. The use of bacteria labeled with 3H-glucose offers a possibility of an objective rapid and precise determination of the capacity of the microbes to adhere to the cell surface."}
{"id": "PMID:1092109", "title": "[A method of determining the efficiency of the projected process of uninterrupted microorganism cultivation for vaccine production].", "content": "The authors suggest the method of study of the process of periodic cultivation of microorganisms for prognostication of the optimal process of biomass accumulation at the level of microbial population, homogenous by the spectrum of biochemical properties. A regimen of continuous growth of the microorganisms (calculated with the use of this method), corresponding to the phase of delay of the growth rate, was economically more effective in comparison with the traditional process used in the vaccine production.", "contents": "[A method of determining the efficiency of the projected process of uninterrupted microorganism cultivation for vaccine production]. The authors suggest the method of study of the process of periodic cultivation of microorganisms for prognostication of the optimal process of biomass accumulation at the level of microbial population, homogenous by the spectrum of biochemical properties. A regimen of continuous growth of the microorganisms (calculated with the use of this method), corresponding to the phase of delay of the growth rate, was economically more effective in comparison with the traditional process used in the vaccine production."}
{"id": "PMID:1092110", "title": "[Antibodies to type antigens of group A streptococcal cell walls in erysipelas patients].", "content": "The passive hemagglutination test was used to determine antibodies to type antigens of the cell wall of streptococcus group A in 167 patients suffering from erysipelas, from whom 488 sera were obtained. Antibodies were revealed in 97.2% of the sera. There was a definite relationship between the clinical form of the disease and the level of antibodies: their lowest level and the absence of elevation in the course of the disease was noted in continuous-relapsing form of erysipelas; the appearance of antibodies to the new types of streptococcus was usually noted in late relapses of the disease, which permitted to attribute their occurrence to infection by the new types of the microbes. In individual cases the appearance of antibodies to the new types of streptococcus was observed at the early relapses of erysipelas; this could be explained by the exacerbation of a focus of chronic (endogenous) infection in the skin in the absence of reinfection. Continuous prophylactic administration of bicillin-5 produced no depressive effect on the antibody level in patients with relapsing erysipelas.", "contents": "[Antibodies to type antigens of group A streptococcal cell walls in erysipelas patients]. The passive hemagglutination test was used to determine antibodies to type antigens of the cell wall of streptococcus group A in 167 patients suffering from erysipelas, from whom 488 sera were obtained. Antibodies were revealed in 97.2% of the sera. There was a definite relationship between the clinical form of the disease and the level of antibodies: their lowest level and the absence of elevation in the course of the disease was noted in continuous-relapsing form of erysipelas; the appearance of antibodies to the new types of streptococcus was usually noted in late relapses of the disease, which permitted to attribute their occurrence to infection by the new types of the microbes. In individual cases the appearance of antibodies to the new types of streptococcus was observed at the early relapses of erysipelas; this could be explained by the exacerbation of a focus of chronic (endogenous) infection in the skin in the absence of reinfection. Continuous prophylactic administration of bicillin-5 produced no depressive effect on the antibody level in patients with relapsing erysipelas."}
{"id": "PMID:1092111", "title": "The effect of platelet function inhibitors on experimental venous thrombosis formation in rabbits.", "content": "Inhibitors of the platelet functions, collagen-induced aggregation and release reaction (ASA and sulfinpyrazone) and ADP-induced aggregation (dipyridamole and VK 744), were investigated as potential preventive agents against venous thrombosis formation in rabbits. An experimental model combining endothelial damage (sodium morrhuate) and controlled stasis of the femoral venous flow was used. None of the agents showed any significant protective effect against venous thrombosis, a finding which is in keeping with the results of recent clinical investigation.", "contents": "The effect of platelet function inhibitors on experimental venous thrombosis formation in rabbits. Inhibitors of the platelet functions, collagen-induced aggregation and release reaction (ASA and sulfinpyrazone) and ADP-induced aggregation (dipyridamole and VK 744), were investigated as potential preventive agents against venous thrombosis formation in rabbits. An experimental model combining endothelial damage (sodium morrhuate) and controlled stasis of the femoral venous flow was used. None of the agents showed any significant protective effect against venous thrombosis, a finding which is in keeping with the results of recent clinical investigation."}
{"id": "PMID:1092112", "title": "Plasma substitute induced impairment of the reticuloendothelial system function.", "content": "Currently used plasma substitutes are large colloids and are partly engulfed by reticuloendothelial (RE) cells. This study was designed to investigate the magnitude and duration of the possible RES dysfunction induced by plasma substitutes. The following substances were studied in both normal and burned (LD-10) CBA mice: allogen albumin 3%, and commercial preparations of dextran 40, dextran 70, hydroxyethyl starch, gelatin and PVP. Infusion volume was 20 ml/kg and i.v. infusion time 1 hour. Animals given allogen plasma served as controls. One, 3 and 6 hours after the i.v. injection of 51-Cr-rabbit RBC, we determined the disappearance rate, and uptake and distribution of the 51-Cr-RBC in the main RES organs (liver, spleen and lungs). In normal mice the disappearance rate was significantly decreased one and three hours post infusion, while it was decreased even after 6 hours in burned mice. Groups given albumin or plasma stayed close to non-infused animals. In order to clarify the physiologic significance of these findings we used another test substance known to be handled by the RES. Salmonella enteritidis endotoxin was tested with regard both to toxicity and rate of elimination. The toxicity, after i.p. injection, was significantly intensified both one and 3 hours post-infusion in normal mice. In burned mice toxicity became even more intense. The ability to eliminate 51-Cr-labelled endotoxin was significantly decreased following infusion in both normal and burned mice, one as well as three hours later. The in vitro liver phagocytosis was tested utilizing the liver slice technique. Standardized slices were incubated in Krebs-Ringer buffer together with 125-I-HSA aggregates as RES test substance with and without plasma from mice given plasma substitute infusions. Allogen plasma enhanced the phagocytic uptake (four times), while post-infusion plasma did not. It is concluded from these experiments, utilizing two different RES test substances in both normal and hypovolemic animals, that the plasma expanders tested do impair RES phagocytosis. The mechanism seems to be an interaction with phagocytosis-promoting plasma factors. Even though this impairment is temporary, it may jeopardize such important RES functions as elimination of microorganisms, toxins, cell debris and tumour cells, or spoil the afferent limb of the immune response to many antigens.", "contents": "Plasma substitute induced impairment of the reticuloendothelial system function. Currently used plasma substitutes are large colloids and are partly engulfed by reticuloendothelial (RE) cells. This study was designed to investigate the magnitude and duration of the possible RES dysfunction induced by plasma substitutes. The following substances were studied in both normal and burned (LD-10) CBA mice: allogen albumin 3%, and commercial preparations of dextran 40, dextran 70, hydroxyethyl starch, gelatin and PVP. Infusion volume was 20 ml/kg and i.v. infusion time 1 hour. Animals given allogen plasma served as controls. One, 3 and 6 hours after the i.v. injection of 51-Cr-rabbit RBC, we determined the disappearance rate, and uptake and distribution of the 51-Cr-RBC in the main RES organs (liver, spleen and lungs). In normal mice the disappearance rate was significantly decreased one and three hours post infusion, while it was decreased even after 6 hours in burned mice. Groups given albumin or plasma stayed close to non-infused animals. In order to clarify the physiologic significance of these findings we used another test substance known to be handled by the RES. Salmonella enteritidis endotoxin was tested with regard both to toxicity and rate of elimination. The toxicity, after i.p. injection, was significantly intensified both one and 3 hours post-infusion in normal mice. In burned mice toxicity became even more intense. The ability to eliminate 51-Cr-labelled endotoxin was significantly decreased following infusion in both normal and burned mice, one as well as three hours later. The in vitro liver phagocytosis was tested utilizing the liver slice technique. Standardized slices were incubated in Krebs-Ringer buffer together with 125-I-HSA aggregates as RES test substance with and without plasma from mice given plasma substitute infusions. Allogen plasma enhanced the phagocytic uptake (four times), while post-infusion plasma did not. It is concluded from these experiments, utilizing two different RES test substances in both normal and hypovolemic animals, that the plasma expanders tested do impair RES phagocytosis. The mechanism seems to be an interaction with phagocytosis-promoting plasma factors. Even though this impairment is temporary, it may jeopardize such important RES functions as elimination of microorganisms, toxins, cell debris and tumour cells, or spoil the afferent limb of the immune response to many antigens."}
{"id": "PMID:1092113", "title": "Immunoglobulin synthesis and its induction in B-lymphoid cells.", "content": "Several aspects of synthesis and assembly of Ig molecules in small lymphocytes and in differentiated plasma cells are summarized. Some attempt has been made to analyse at the molecular level what happens on conversion of a lymphocyte to a mature cell secreting high levels of Ig. For this study LPS activation of murine B-lymphocytes into division and maturation served as a model system. A rapid rate of mitosis and high rate of H-mRNA synthesis appeared to precede the high levels of Ig production, suggesting a translational block on H-mRNA until the cell had organized its endoplasmic reticulum and matured. In vivo, with the exception of T-independent antigens, it is unlikely that B-cell maturationis an automatic sequel to division stimuli. We are largely ignorant in this area but possible regulatory signals which achieve a dissociation of B-cell division and maturation are discussed.", "contents": "Immunoglobulin synthesis and its induction in B-lymphoid cells. Several aspects of synthesis and assembly of Ig molecules in small lymphocytes and in differentiated plasma cells are summarized. Some attempt has been made to analyse at the molecular level what happens on conversion of a lymphocyte to a mature cell secreting high levels of Ig. For this study LPS activation of murine B-lymphocytes into division and maturation served as a model system. A rapid rate of mitosis and high rate of H-mRNA synthesis appeared to precede the high levels of Ig production, suggesting a translational block on H-mRNA until the cell had organized its endoplasmic reticulum and matured. In vivo, with the exception of T-independent antigens, it is unlikely that B-cell maturationis an automatic sequel to division stimuli. We are largely ignorant in this area but possible regulatory signals which achieve a dissociation of B-cell division and maturation are discussed."}
{"id": "PMID:1092115", "title": "Effects of immunization with LDH-X on fertility.", "content": "Immunological regulation of fertility can be best achieved if a defined macromolecule is used to provide the antigenic stimulus. Proteins with known enzymatic properties are attractive molecules in this area if it can be demonstrated unequivocally that they are unique constituents of spermatozoa, and are completely foreign to the female. This paper describes immunosuppression of fertility by the sperm-specific isozyme of lactate dehydrogenase (LDH-X). In addition, evidence is reviewed which suggests that acrosomal proteinase and hyaluronidase may be unique to sperm. Data are presented which confirm the sperm specificity of LDH-X and the immunochemical homogeneity of purified preparations of this isozyme. Immunization of female rabbits with LDH-X significantly reduces the fertility of these animals. Experimental results indicate that the primary effect of immunization involves blockage of fertilization. While those ova which are fertilized in an immune environment develop and implant normally, there is a high rate of post-implantation embryo mortality. Rabbit blastocysts transferred to immune recipients implant at the same rate as in non-immune controls. These findings are consistent with the conclusion that the developing embryo must interact with antibody during the pre-implantation stages of pregnancy when antigenic sites conferred by the sperm LDH-X are available. Such interaction can be demonstrated directly by immunofluorescence techniques.", "contents": "Effects of immunization with LDH-X on fertility. Immunological regulation of fertility can be best achieved if a defined macromolecule is used to provide the antigenic stimulus. Proteins with known enzymatic properties are attractive molecules in this area if it can be demonstrated unequivocally that they are unique constituents of spermatozoa, and are completely foreign to the female. This paper describes immunosuppression of fertility by the sperm-specific isozyme of lactate dehydrogenase (LDH-X). In addition, evidence is reviewed which suggests that acrosomal proteinase and hyaluronidase may be unique to sperm. Data are presented which confirm the sperm specificity of LDH-X and the immunochemical homogeneity of purified preparations of this isozyme. Immunization of female rabbits with LDH-X significantly reduces the fertility of these animals. Experimental results indicate that the primary effect of immunization involves blockage of fertilization. While those ova which are fertilized in an immune environment develop and implant normally, there is a high rate of post-implantation embryo mortality. Rabbit blastocysts transferred to immune recipients implant at the same rate as in non-immune controls. These findings are consistent with the conclusion that the developing embryo must interact with antibody during the pre-implantation stages of pregnancy when antigenic sites conferred by the sperm LDH-X are available. Such interaction can be demonstrated directly by immunofluorescence techniques."}
{"id": "PMID:1092116", "title": "Macrophage activation induced by lymphocyte mediators.", "content": "This paper is a brief review of studies which demonstrate that lymphocyte mediators can activate macrophages in vitro. Macrophages which have been incubated in lymphocyte mediator-rich Sephadex fractions show changed morphology, metabolism and function. These changes include an increase in adherence to glass. ruffled membrane movement, phagocytosis of some particles, glucose oxidation through the hexose monophosphate shunt and an increase in the activity of a membrane enzyme, adenylate cyclase. Such mediator-activated macrophages show enhanced bacteriostasis and tumoricidal activity. In addition, studies describing the role of membrane sugars and esterases in the interaction of migration inhibition factor and macrophages are reviewed.", "contents": "Macrophage activation induced by lymphocyte mediators. This paper is a brief review of studies which demonstrate that lymphocyte mediators can activate macrophages in vitro. Macrophages which have been incubated in lymphocyte mediator-rich Sephadex fractions show changed morphology, metabolism and function. These changes include an increase in adherence to glass. ruffled membrane movement, phagocytosis of some particles, glucose oxidation through the hexose monophosphate shunt and an increase in the activity of a membrane enzyme, adenylate cyclase. Such mediator-activated macrophages show enhanced bacteriostasis and tumoricidal activity. In addition, studies describing the role of membrane sugars and esterases in the interaction of migration inhibition factor and macrophages are reviewed."}
{"id": "PMID:1092117", "title": "Local immunological response in the vagina, cervix and endometrium.", "content": "The mechanism of the local excretion of secretory IgA (SIgA) in exocrine secretions has been reviewed. Numerous local IgA-plasma cells, in the lamina propria of the glandular mucosa, synthesize dimeric IgA with J-chain. Free secretory component (FSC) is synthesized and accumulated in the Golgi area of the columnar epithelial cells. It is then supposed to get onto their cell membranes. Dimeric IgA (and some IgM) reaches tn dimeric IgA (and some IgM) and the FSC located in the cell-membrane, whereby SIgA is formed. The complex becomes mobilized and is transported toward the apical part of the cell, where it will be excreted into the mucous coat covering the epithelium. In the female genital tract, the cervical mucosa appears to be better adapted to achieve a local secretory immune system. The endometrium seems less suitable, being normally short of local plasma cells. The vaginal wall appears almost incompatible with the proposed mechanism of local antibody secretion. Criteria for establishing a local immune response in the female genital tract comprise: 1) a lack of correlation between antibody titers in secretions and serum; 2) the demonstration that the secretory antibodies are mainly of IgA class and 3) that they are SIgA molecules, possessing bound secretory component. However, the best criterion would be 4) the observation that antibody is actually synthesized in samples of mucosa, by in vitro culture or immunohistology. Reviewing the literature, relatively few examples were found where SIgA antibodies were demonstrated, and unambiguous evidence for their local synthesis is almost non-existent. In addition, the authors were unable to detect antibody-containing cells in cervical and endometrial biopsies of women locally \"immunized\" with horse spleen ferritin and bovine serum albumin. The need for further investigation with simple antigens and adequate immunological reagents is stressed.", "contents": "Local immunological response in the vagina, cervix and endometrium. The mechanism of the local excretion of secretory IgA (SIgA) in exocrine secretions has been reviewed. Numerous local IgA-plasma cells, in the lamina propria of the glandular mucosa, synthesize dimeric IgA with J-chain. Free secretory component (FSC) is synthesized and accumulated in the Golgi area of the columnar epithelial cells. It is then supposed to get onto their cell membranes. Dimeric IgA (and some IgM) reaches tn dimeric IgA (and some IgM) and the FSC located in the cell-membrane, whereby SIgA is formed. The complex becomes mobilized and is transported toward the apical part of the cell, where it will be excreted into the mucous coat covering the epithelium. In the female genital tract, the cervical mucosa appears to be better adapted to achieve a local secretory immune system. The endometrium seems less suitable, being normally short of local plasma cells. The vaginal wall appears almost incompatible with the proposed mechanism of local antibody secretion. Criteria for establishing a local immune response in the female genital tract comprise: 1) a lack of correlation between antibody titers in secretions and serum; 2) the demonstration that the secretory antibodies are mainly of IgA class and 3) that they are SIgA molecules, possessing bound secretory component. However, the best criterion would be 4) the observation that antibody is actually synthesized in samples of mucosa, by in vitro culture or immunohistology. Reviewing the literature, relatively few examples were found where SIgA antibodies were demonstrated, and unambiguous evidence for their local synthesis is almost non-existent. In addition, the authors were unable to detect antibody-containing cells in cervical and endometrial biopsies of women locally \"immunized\" with horse spleen ferritin and bovine serum albumin. The need for further investigation with simple antigens and adequate immunological reagents is stressed."}
{"id": "PMID:1092119", "title": "Fertility control through active immunization using placenta proteins.", "content": "Numerous studies have demonstrated that antibodies to placental proteins in a variety of species are capable of preventing or disrupting gestation. Early work in this area was primarily directed towards the passive immunization of rodents with heterologous antisera to whole placental extracts. Toxicity and renal damage often accompanied fertility inhibition. More recent studies reported less toxicity and a higher specificity of antibodies to reproductive function when anti-placental antibodies were absorbed with serum and extracts of non-reproductive organs. Few studies have been reported in which active immunization with placental proteins was employed. The most detailed studies of active immunization have employed highly purified placental hormones. Immunizations of rats and rabbits with human placental lactogen have resulted in marked reduction in reproductive function. Immunization of human females with chemically altered (hapten-coupled/ HCG resulted in the production of antibodies reacting with unaltered HCG and pituitary LH. These antibodies were capable of reducing the level of endogenous serum LH in pre- and post-menopausal women. The also altered the events of the menstrual cycle in premenopausal women. More specific inhibition of chorionic gonadotrophin has been obtained by immunization of baboons with the beta subunit of HCG. Antifertility effects without alterations in the menstrual cycle of female baboons immunized with the beta subunit of HCG have been reported. The antibodies produced in these animals reacted significantly with human LH in vitro. The possibility of using hormonal and non-hormonal placental proteins as antigens for the specific immunological inhibition of fertility remains.", "contents": "Fertility control through active immunization using placenta proteins. Numerous studies have demonstrated that antibodies to placental proteins in a variety of species are capable of preventing or disrupting gestation. Early work in this area was primarily directed towards the passive immunization of rodents with heterologous antisera to whole placental extracts. Toxicity and renal damage often accompanied fertility inhibition. More recent studies reported less toxicity and a higher specificity of antibodies to reproductive function when anti-placental antibodies were absorbed with serum and extracts of non-reproductive organs. Few studies have been reported in which active immunization with placental proteins was employed. The most detailed studies of active immunization have employed highly purified placental hormones. Immunizations of rats and rabbits with human placental lactogen have resulted in marked reduction in reproductive function. Immunization of human females with chemically altered (hapten-coupled/ HCG resulted in the production of antibodies reacting with unaltered HCG and pituitary LH. These antibodies were capable of reducing the level of endogenous serum LH in pre- and post-menopausal women. The also altered the events of the menstrual cycle in premenopausal women. More specific inhibition of chorionic gonadotrophin has been obtained by immunization of baboons with the beta subunit of HCG. Antifertility effects without alterations in the menstrual cycle of female baboons immunized with the beta subunit of HCG have been reported. The antibodies produced in these animals reacted significantly with human LH in vitro. The possibility of using hormonal and non-hormonal placental proteins as antigens for the specific immunological inhibition of fertility remains."}
{"id": "PMID:1092120", "title": "The use of antibodies developed by infertile women to identify relevant antigens.", "content": "In the first half of this century several studies demonstrated that temporary infertility may be induced by immunizing women with semen. Isolated examples of acute hypersensitivity reactions to sexual intercourse provide dramatic evidence that natural isoimmunization to semen occurs in man. The afferent immunological pathway in this process can be influenced by adjuvant effects of genital tract infection. This may be important in assessing the significance of antisperm antibodies in infertile women. In immunological studies of female infertility, the 3 most commonly used tests for humoral antisperm activity are microagglutination, macroagglutination and immobilization (complement dependent cytotoxicity). The sperm microagglutination test detects both antibody and non-antibody activity. The macroagglutination and immobilization tests both detect antibodies but to date the latter has provided the more meaningful results in infertile women. A prospective study of reproductive patterns in infertility patients indicated a lower subsequent fertility rate and a markedly higher abortion rate in women with antisperm antibodies compared to those without them. However, the association of antisperm antibodies with continuing infertility only reached a statistically significant level in women with a past history of unexplained infertility of at least 3 years' duration. Occlusion (condom) therapy in patients with antisperm antibodies has proved unsuccessful when compared with the natural reproductive patterns in untreated patients. Immunofluorescence studies are described and correlated with results of other tests for antisperm antibodies in serum and cerivcal mucus. The results of these and of other studies of naturally occurring antibodies in infertile women have identified antigens of actual or potential relevance to infertility and fertility control. These include (i) intrinsic spermatozoal antigens, (ii) seminal plasma antigens, (iii) blood group antigens, (iv) histocompatibility antigens and (v) nucleoprotein antigens.", "contents": "The use of antibodies developed by infertile women to identify relevant antigens. In the first half of this century several studies demonstrated that temporary infertility may be induced by immunizing women with semen. Isolated examples of acute hypersensitivity reactions to sexual intercourse provide dramatic evidence that natural isoimmunization to semen occurs in man. The afferent immunological pathway in this process can be influenced by adjuvant effects of genital tract infection. This may be important in assessing the significance of antisperm antibodies in infertile women. In immunological studies of female infertility, the 3 most commonly used tests for humoral antisperm activity are microagglutination, macroagglutination and immobilization (complement dependent cytotoxicity). The sperm microagglutination test detects both antibody and non-antibody activity. The macroagglutination and immobilization tests both detect antibodies but to date the latter has provided the more meaningful results in infertile women. A prospective study of reproductive patterns in infertility patients indicated a lower subsequent fertility rate and a markedly higher abortion rate in women with antisperm antibodies compared to those without them. However, the association of antisperm antibodies with continuing infertility only reached a statistically significant level in women with a past history of unexplained infertility of at least 3 years' duration. Occlusion (condom) therapy in patients with antisperm antibodies has proved unsuccessful when compared with the natural reproductive patterns in untreated patients. Immunofluorescence studies are described and correlated with results of other tests for antisperm antibodies in serum and cerivcal mucus. The results of these and of other studies of naturally occurring antibodies in infertile women have identified antigens of actual or potential relevance to infertility and fertility control. These include (i) intrinsic spermatozoal antigens, (ii) seminal plasma antigens, (iii) blood group antigens, (iv) histocompatibility antigens and (v) nucleoprotein antigens."}
{"id": "PMID:1092122", "title": "Kinetics of antibody formation and regulatory aspects of immunity.", "content": "This paper, which is to be read in conjunction with that of Dr. J.F.A.P. Miller earlier in this volume, addresses itself to the kinetics of antibody production and to the regulation of the immune response. It is divided into four main sections. The first examines the kinetics of antibody production to a single, strong challenge injection of antigen. The cellular events involved in the latent period and the subsequent exponential phase of antibody production are examined from the viewpoint of four key concepts: triggering, clonal expansion, IgM to IgG switch and affinity maturation. The second section examines the major regulatory influences and feedback loops in immunity. Antigen is discussed as an agent capable of causing tolerance as well as triggering. Antibody is considered an important regulator, and the mechanisms of IgM-positive feedback, IgG-negative feedback, and tolerance and blockade effects of immune complexes are outlined. Immunological memory, the opposite phenomenon to tolerance, is briefly described, as is the importance of genetic factors. The third sections looks at more complex models of the immune response, and in particular at the lessons to be learnt from tumour progression.", "contents": "Kinetics of antibody formation and regulatory aspects of immunity. This paper, which is to be read in conjunction with that of Dr. J.F.A.P. Miller earlier in this volume, addresses itself to the kinetics of antibody production and to the regulation of the immune response. It is divided into four main sections. The first examines the kinetics of antibody production to a single, strong challenge injection of antigen. The cellular events involved in the latent period and the subsequent exponential phase of antibody production are examined from the viewpoint of four key concepts: triggering, clonal expansion, IgM to IgG switch and affinity maturation. The second section examines the major regulatory influences and feedback loops in immunity. Antigen is discussed as an agent capable of causing tolerance as well as triggering. Antibody is considered an important regulator, and the mechanisms of IgM-positive feedback, IgG-negative feedback, and tolerance and blockade effects of immune complexes are outlined. Immunological memory, the opposite phenomenon to tolerance, is briefly described, as is the importance of genetic factors. The third sections looks at more complex models of the immune response, and in particular at the lessons to be learnt from tumour progression."}
{"id": "PMID:1092125", "title": "Production of lymphoid tissue in the rat brain by implants containing phytohemagglutinin.", "content": "Large numbers of plasmacytes and lymphocytic cells develop at the tip of miniature pipettes containing phytohemagglutinin (PHA) implanted into the brain of adult Lewis rats. The accumulation of lymphoid elements is present in tissue exposed to PHA for 1 week, and it persists in animals which were allowed to survive up to 10 weeks. It did not occur around control or non-active implants. Lymphoid cells usually serve as a morphologic index of immune mediated phenomena, but no evidence was seen in the present study to indicate that they produce damage to axons, dendrites or glial cells of the central nervous system despite direct contact with them. Plasma cells in the brain appear to develop from \"dark\" cells resembling lymphocytes that migrate into the central nervous system from reactive lymphoid tissue near the tip o.f the implant. Nerve, glial and subependymal cells do not respond with mitogenic activity to PHA.", "contents": "Production of lymphoid tissue in the rat brain by implants containing phytohemagglutinin. Large numbers of plasmacytes and lymphocytic cells develop at the tip of miniature pipettes containing phytohemagglutinin (PHA) implanted into the brain of adult Lewis rats. The accumulation of lymphoid elements is present in tissue exposed to PHA for 1 week, and it persists in animals which were allowed to survive up to 10 weeks. It did not occur around control or non-active implants. Lymphoid cells usually serve as a morphologic index of immune mediated phenomena, but no evidence was seen in the present study to indicate that they produce damage to axons, dendrites or glial cells of the central nervous system despite direct contact with them. Plasma cells in the brain appear to develop from \"dark\" cells resembling lymphocytes that migrate into the central nervous system from reactive lymphoid tissue near the tip o.f the implant. Nerve, glial and subependymal cells do not respond with mitogenic activity to PHA."}
{"id": "PMID:1092127", "title": "Persistent neonatal hypoglycaemia. A clinical and histopathological study of three cases treated with diazoxide and subtotal pancreatectomy.", "content": "Two boys and one girl developed persisting hypoglycaemia 12, 24, and 48 hours after birth. Although there was no known history of hereditary diabetes mellitus, the birth weight was high in two cases, and some additional traits of foetopathia diabetica could not be excluded clinically. All had high serum insulin levels with frank hyperinsulinaemia in one case. Glucose tolerance tests also indicated hyperinsulinism. Diazoxide (8 to 27 mg/kg) elevated the blood glucose levels, but did not prevent severe hypoglycaemic episodes. The effects of subtotal pancreatectomy were only transient. The patients have now been kept on permanent diazoxide therapy for 2-4 years at dosages slightly lower than those used before operation. No islet-cell tumour was found at the subtotal pancreatectomy. In all 3 cases, the pancreatic islets were markedly hyperplastic and of irregular shape with the occurrence of large B-cells with giant hyperchromatic nuclei and chromophobe \"agranular\" or sparsely granulated cells. The predominating kind of islet cells showed tinctorial features of A2-cells but--in the absence of available material set aside for ultrastructural analysis--it could not be settled whether this was a result of a proliferation of A2-cells only or of \"type IV cells\" as well. Against the background of previously published reports, the present cases serve to illustrate that additional accuracy of diagnosis and classification of neonatal persistent hypoglycaemia requires quantitative information about the structural changes in the pancreatic islet cells, and that this can be obtained from conventional biopsy specimens.", "contents": "Persistent neonatal hypoglycaemia. A clinical and histopathological study of three cases treated with diazoxide and subtotal pancreatectomy. Two boys and one girl developed persisting hypoglycaemia 12, 24, and 48 hours after birth. Although there was no known history of hereditary diabetes mellitus, the birth weight was high in two cases, and some additional traits of foetopathia diabetica could not be excluded clinically. All had high serum insulin levels with frank hyperinsulinaemia in one case. Glucose tolerance tests also indicated hyperinsulinism. Diazoxide (8 to 27 mg/kg) elevated the blood glucose levels, but did not prevent severe hypoglycaemic episodes. The effects of subtotal pancreatectomy were only transient. The patients have now been kept on permanent diazoxide therapy for 2-4 years at dosages slightly lower than those used before operation. No islet-cell tumour was found at the subtotal pancreatectomy. In all 3 cases, the pancreatic islets were markedly hyperplastic and of irregular shape with the occurrence of large B-cells with giant hyperchromatic nuclei and chromophobe \"agranular\" or sparsely granulated cells. The predominating kind of islet cells showed tinctorial features of A2-cells but--in the absence of available material set aside for ultrastructural analysis--it could not be settled whether this was a result of a proliferation of A2-cells only or of \"type IV cells\" as well. Against the background of previously published reports, the present cases serve to illustrate that additional accuracy of diagnosis and classification of neonatal persistent hypoglycaemia requires quantitative information about the structural changes in the pancreatic islet cells, and that this can be obtained from conventional biopsy specimens."}
{"id": "PMID:1092128", "title": "Experimental murine leprosy. 3. Early local reaction to mycobacterium lepraemurium in C3H and C57/BL mice.", "content": "M. lepraemurium was injected subcutaneously into two inbred strains of mice, C3H and C57/BL, in order to study the local reaction at various time intervals. Within 6 hours an acute inflammatory reaction developed at the site of injection. In the course of the following days it was replaced by a mononuclear infiltrate. The influx of mononuclear cells appeared to be somewhat greater in C57/BL than in C3H mice. Apart from this, little difference was observed between the two strains until at 4 weeks when a vigorous granulomatous reaction developed in the C57/BL strain. This reaction apparently arrested further local spread of the infection. The histological appearance of the inflitrate indicated that a delayed hypersensitivity reaction was taking place. No signs of such reaction was observed in the C3H strain.", "contents": "Experimental murine leprosy. 3. Early local reaction to mycobacterium lepraemurium in C3H and C57/BL mice. M. lepraemurium was injected subcutaneously into two inbred strains of mice, C3H and C57/BL, in order to study the local reaction at various time intervals. Within 6 hours an acute inflammatory reaction developed at the site of injection. In the course of the following days it was replaced by a mononuclear infiltrate. The influx of mononuclear cells appeared to be somewhat greater in C57/BL than in C3H mice. Apart from this, little difference was observed between the two strains until at 4 weeks when a vigorous granulomatous reaction developed in the C57/BL strain. This reaction apparently arrested further local spread of the infection. The histological appearance of the inflitrate indicated that a delayed hypersensitivity reaction was taking place. No signs of such reaction was observed in the C3H strain."}
{"id": "PMID:1092129", "title": "Experimental murine leprosy. 4. The gross appearance and microscopic features of the local infiltrate after subcutaneous inoculation of C3H and C57/BL mice with mycobacterium lepraemurium.", "content": "Mice with the inbred strains C57/BL and C3H were inoculated subcutaneously on the thorax with M. lepraemurium. In C57/BL mice a firm, raised, sharply defined nodular infiltrate developed 4 weeks afterwards, while in the C3H strain of the infection produced a soft, flattened infiltrate with ill-defined margins, which did not become palpable until 10 weeks after inoculation. A limited spread of the infection occurred early in both strains, but apparently multiplication of the microorganisms was very restricted in C57/BL mice; progressive, disseminated growth of the bacilli was observed in the C3H strain only. In C57/BL mice the granulomatous reaction, developing 4 weeks after inoculation and leading to abcess formation, ulceration and scar formation, apparently inhibited both local multiplication and further spread of the bacilli. In C3H mice no host reaction was detected and the bacilli appeared to grow unrestrictedly. In some C57/BL animals, decrease in host resistance occurred during the infection, causing reactivation of the local lesion and an apparently rapid proliferation of bacilli. Observations regarding the lesions in superinfected animals indicated that a systemic immune reaction develops in the C57/BL strain about 4 weeks after inoculation, whereas this does not occur in the C3H strain.", "contents": "Experimental murine leprosy. 4. The gross appearance and microscopic features of the local infiltrate after subcutaneous inoculation of C3H and C57/BL mice with mycobacterium lepraemurium. Mice with the inbred strains C57/BL and C3H were inoculated subcutaneously on the thorax with M. lepraemurium. In C57/BL mice a firm, raised, sharply defined nodular infiltrate developed 4 weeks afterwards, while in the C3H strain of the infection produced a soft, flattened infiltrate with ill-defined margins, which did not become palpable until 10 weeks after inoculation. A limited spread of the infection occurred early in both strains, but apparently multiplication of the microorganisms was very restricted in C57/BL mice; progressive, disseminated growth of the bacilli was observed in the C3H strain only. In C57/BL mice the granulomatous reaction, developing 4 weeks after inoculation and leading to abcess formation, ulceration and scar formation, apparently inhibited both local multiplication and further spread of the bacilli. In C3H mice no host reaction was detected and the bacilli appeared to grow unrestrictedly. In some C57/BL animals, decrease in host resistance occurred during the infection, causing reactivation of the local lesion and an apparently rapid proliferation of bacilli. Observations regarding the lesions in superinfected animals indicated that a systemic immune reaction develops in the C57/BL strain about 4 weeks after inoculation, whereas this does not occur in the C3H strain."}
{"id": "PMID:1092132", "title": "Liver impairment during chronic hemodialysis and after renal transplantation.", "content": "Liver impairment has been evaluated in a consecutive series of 79 patients with chronic renal failure of whom 23 were treated with hemodialysis alone and 37 with hemodialysis and renal transplantation alone. In half of the chronic hemodialysis patients and half of the patients receiving a renal allograft elevation of serum alanine aminotransferases was observed for a shorter or longer period during the study. In half of these cases from both groups the clinical course, laboratory data and liver histology were consistent with virus hepatitis and four patients died from fulminant hepatic failure. In the other half of the patients with elevated transaminases, this was either asymptomatic and unexplained or due to other causes such as septicaemia or urinary leakage. Liver biopsy showed unspecific changes. Renal transplantation was not performed in patients suffering from virus hepatitis, but 12 of the 37 patients who received a renal allograft had elevated aminotransferases at the time of transplantation. In seven of them a marked increase in aminotransferase was observed postoperatively, but none developed clinical sign of liver disease. It is concluded that elevated aminotransferase activity per se is no contraindication to surgical procedures, including renal transplatation, in these patients. However, a liver biopsy should be performed to detect a possible liver disease.", "contents": "Liver impairment during chronic hemodialysis and after renal transplantation. Liver impairment has been evaluated in a consecutive series of 79 patients with chronic renal failure of whom 23 were treated with hemodialysis alone and 37 with hemodialysis and renal transplantation alone. In half of the chronic hemodialysis patients and half of the patients receiving a renal allograft elevation of serum alanine aminotransferases was observed for a shorter or longer period during the study. In half of these cases from both groups the clinical course, laboratory data and liver histology were consistent with virus hepatitis and four patients died from fulminant hepatic failure. In the other half of the patients with elevated transaminases, this was either asymptomatic and unexplained or due to other causes such as septicaemia or urinary leakage. Liver biopsy showed unspecific changes. Renal transplantation was not performed in patients suffering from virus hepatitis, but 12 of the 37 patients who received a renal allograft had elevated aminotransferases at the time of transplantation. In seven of them a marked increase in aminotransferase was observed postoperatively, but none developed clinical sign of liver disease. It is concluded that elevated aminotransferase activity per se is no contraindication to surgical procedures, including renal transplatation, in these patients. However, a liver biopsy should be performed to detect a possible liver disease."}
{"id": "PMID:1092133", "title": "Transfer of E1 and V colicinogenic factors to Shigella flexneri.", "content": "From Escherichia coli strains, ColE1-ML and ColV-K94 factors were transferred to a virulent Shigella flexneri 2a strain at 1-2% frequency. S. flexneri ColV+ and ColE1+V+ produced Col- segregants. The ColE1 factor was stably incorporated into S. flexneri. The ColV+ culture lost its virulence to the guinea pig eye.", "contents": "Transfer of E1 and V colicinogenic factors to Shigella flexneri. From Escherichia coli strains, ColE1-ML and ColV-K94 factors were transferred to a virulent Shigella flexneri 2a strain at 1-2% frequency. S. flexneri ColV+ and ColE1+V+ produced Col- segregants. The ColE1 factor was stably incorporated into S. flexneri. The ColV+ culture lost its virulence to the guinea pig eye."}
{"id": "PMID:1092134", "title": "Effect of some aminoglycosides upon a drug dependent Escherichia coli strain.", "content": "Escherichia coli Sd-4-73 drug dependent strain was sensitive to kanamycin, gentamicin and neomycin in the absence of drugs stimulating cell multiplication. This senstivity was not considerably influenced by streptomycin, while paromomycin afforded protection against both bacteriostatic and bactericidal effects. The paromomycin concentration optimum for protection was different from its concentration optimum for multiplication.", "contents": "Effect of some aminoglycosides upon a drug dependent Escherichia coli strain. Escherichia coli Sd-4-73 drug dependent strain was sensitive to kanamycin, gentamicin and neomycin in the absence of drugs stimulating cell multiplication. This senstivity was not considerably influenced by streptomycin, while paromomycin afforded protection against both bacteriostatic and bactericidal effects. The paromomycin concentration optimum for protection was different from its concentration optimum for multiplication."}
{"id": "PMID:1092130", "title": "Changes in certain iron metabolism variables after a single blood donation.", "content": "Signs of iron deficiency have been studied after the first blood donation in 11 healthy men. Six were given 100 mg iron daily, and five received placebo tablets. The total iron-binding capacity and iron absoprtion remained raised for more than 26 days, but had almost returned to the initial values after 70 days. A significant decrease in the stainable bone marrow iron could be shown in all subjects after 26 days; later some restitution was seen in subjects given iron supplements, but not in those given placebo. As the restitution times are long, the interval after blood donation must be taken into account when judging iron metabolism variables in active donors.", "contents": "Changes in certain iron metabolism variables after a single blood donation. Signs of iron deficiency have been studied after the first blood donation in 11 healthy men. Six were given 100 mg iron daily, and five received placebo tablets. The total iron-binding capacity and iron absoprtion remained raised for more than 26 days, but had almost returned to the initial values after 70 days. A significant decrease in the stainable bone marrow iron could be shown in all subjects after 26 days; later some restitution was seen in subjects given iron supplements, but not in those given placebo. As the restitution times are long, the interval after blood donation must be taken into account when judging iron metabolism variables in active donors."}
{"id": "PMID:1092131", "title": "Iron supplement to blood donors. I. Trials with intermittent iron supply.", "content": "A series of 58 male blood donors has been studied with regard to stainable bone marrow iron, desferrioxamine test, sideroblasts, total iron-binding capacity, serum iron, and haemoglobin values. With one conventional blood donation every second month the storage iron was found to be significantly decreased after four blood donations both when placebo and oral ferrous iron in doses of up to 2000 mg were given over a period of two weeks after each blood-letting. The iron state in donors given 2000 mg was superior to that in donors given 1000 mg, and better in the latter group than when placebo was given. A moderate increase in the total iron-binding capacity could be discerned in subjects treated with placebo or only 1000 mg iron after each donation, but no changes in serum iron or haemoglobin were noted. A smaller series of 13 donors was also investigated after six donations and was found to show essentially the same pattern in the iron state variables as after four donations. When the interval between donations was four months and 2000 mg ferrous iron was given over two weeks after each donation, all variables including the amount of bone marrow iron and the desferrioxamine test remained unchanged in ten subjects after four donations. Two subjects showed a moderate decrease in the stainable bone marrow iron, but it did not disappear completely.", "contents": "Iron supplement to blood donors. I. Trials with intermittent iron supply. A series of 58 male blood donors has been studied with regard to stainable bone marrow iron, desferrioxamine test, sideroblasts, total iron-binding capacity, serum iron, and haemoglobin values. With one conventional blood donation every second month the storage iron was found to be significantly decreased after four blood donations both when placebo and oral ferrous iron in doses of up to 2000 mg were given over a period of two weeks after each blood-letting. The iron state in donors given 2000 mg was superior to that in donors given 1000 mg, and better in the latter group than when placebo was given. A moderate increase in the total iron-binding capacity could be discerned in subjects treated with placebo or only 1000 mg iron after each donation, but no changes in serum iron or haemoglobin were noted. A smaller series of 13 donors was also investigated after six donations and was found to show essentially the same pattern in the iron state variables as after four donations. When the interval between donations was four months and 2000 mg ferrous iron was given over two weeks after each donation, all variables including the amount of bone marrow iron and the desferrioxamine test remained unchanged in ten subjects after four donations. Two subjects showed a moderate decrease in the stainable bone marrow iron, but it did not disappear completely."}
{"id": "PMID:1092149", "title": "Clinical application of a second generation electrocardiographic computer program.", "content": "An electrocardiographic computer program based on multivariate analysis of orthogonal leads (Frank) was applied to records transmitted daily by telephone from the Veterans Administration Hospital, West Roxbury, Mass., to the Veterans Administration Hospital, Washington, D. C. A Bayesian classification procedure was used to compute probabilities for all diagnostic categories that might be encountered in a given record. Computer results were compared with interpretations of conventional 12 lead tracings. Of 1,663 records transmitted, 1,192 were selected for the study because the clinical diagnosis in these cases could be firmly established on the basis of independent, nonelectrocardiographic information. Twenty-one percent of the records were obtained from patients without evidence of cardiac disease and 79 percent from patients with various cardiovascular illnesses. Diagnostic electrocardiographic classifications were considered correct when in agreement with documented clinical diagnoses. Of the total sample of 1,192 recordings, 86 percent were classified correctly by computer as compared with 68 percent by conventional 12 lead electrocardiographic analysis. Improvement in diagnostic recognition by computer was most striking in patients with hypertensive cardiovascular disease or chronic obstructive lung disease. The multivariate classification scheme functioned most efficiently when a problem-oriented approach to diagnosis was simulated. This was accomplished by a simple method of adjusting prior probabilities according to the diagnostic problem under consideration.", "contents": "Clinical application of a second generation electrocardiographic computer program. An electrocardiographic computer program based on multivariate analysis of orthogonal leads (Frank) was applied to records transmitted daily by telephone from the Veterans Administration Hospital, West Roxbury, Mass., to the Veterans Administration Hospital, Washington, D. C. A Bayesian classification procedure was used to compute probabilities for all diagnostic categories that might be encountered in a given record. Computer results were compared with interpretations of conventional 12 lead tracings. Of 1,663 records transmitted, 1,192 were selected for the study because the clinical diagnosis in these cases could be firmly established on the basis of independent, nonelectrocardiographic information. Twenty-one percent of the records were obtained from patients without evidence of cardiac disease and 79 percent from patients with various cardiovascular illnesses. Diagnostic electrocardiographic classifications were considered correct when in agreement with documented clinical diagnoses. Of the total sample of 1,192 recordings, 86 percent were classified correctly by computer as compared with 68 percent by conventional 12 lead electrocardiographic analysis. Improvement in diagnostic recognition by computer was most striking in patients with hypertensive cardiovascular disease or chronic obstructive lung disease. The multivariate classification scheme functioned most efficiently when a problem-oriented approach to diagnosis was simulated. This was accomplished by a simple method of adjusting prior probabilities according to the diagnostic problem under consideration."}
{"id": "PMID:1092150", "title": "Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.", "content": "A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized \"gonadotropes\". Most, but not all, \"gonadotropes\" show significant nuclear concentration of radioactivity. A large percentage of non-\"gonadotropes\" also show nuclear uptake of label.", "contents": "Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary. A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized \"gonadotropes\". Most, but not all, \"gonadotropes\" show significant nuclear concentration of radioactivity. A large percentage of non-\"gonadotropes\" also show nuclear uptake of label."}
{"id": "PMID:1092151", "title": "Effect of acupuncture of pain threshold and pain tolerance determined by electrical stimulation of the skin: a controlled study.", "content": "The effect of needling traditional acupuncture loci (Ho-Ku, LI4; Nei-Kuan, EH-6) compared to arbitarily chosen points was measured in twelve healthy subjects.  In a crossover blind study pain tolerance and pain threshold were measured by electrical stimulation of  the skin in the thyroid area.  Traditional acupuncture was found to be significantly more effective in elevating pain  threshold than needling of arbitarily chosen points.  Pain tolerance also rose more under \"real\" acupuncture, the difference, however, being statistically not significant.  In one subject, threshold and tolerance increased more under needling of nonacupuncture loci, while two subjects did not show any changes under either procedure.", "contents": "Effect of acupuncture of pain threshold and pain tolerance determined by electrical stimulation of the skin: a controlled study. The effect of needling traditional acupuncture loci (Ho-Ku, LI4; Nei-Kuan, EH-6) compared to arbitarily chosen points was measured in twelve healthy subjects.  In a crossover blind study pain tolerance and pain threshold were measured by electrical stimulation of  the skin in the thyroid area.  Traditional acupuncture was found to be significantly more effective in elevating pain  threshold than needling of arbitarily chosen points.  Pain tolerance also rose more under \"real\" acupuncture, the difference, however, being statistically not significant.  In one subject, threshold and tolerance increased more under needling of nonacupuncture loci, while two subjects did not show any changes under either procedure."}
{"id": "PMID:1092152", "title": "Susceptibilities of bacteria to different antibiotic regimens. Study in two nursery populations.", "content": "Hospital nursery A has used chloramphenicol and nursery B has used the combination of penicillin G sodium and kanamycin sulfate routinely in the treatment of neonatal sepsis and other bacterial infections. A hypothesis was formulated that these different antibiotic pressures would select out a substantial number of populations of resistant bacteria in each of the two nurseries. This was tested by periodic sampling of the skin, mouth, and rectal flora of babies and the permanent personnel in these nurseries. These bacteria were studied for susceptibility to a number of antibiotics. The population of resistant strains selected out was correlated with the antibiotics used in each nursery. There is a need for continuing surveillance of hospital nursery strains of bacteria for in vitro susceptibilities to commonly prescribed antimicrobials.", "contents": "Susceptibilities of bacteria to different antibiotic regimens. Study in two nursery populations. Hospital nursery A has used chloramphenicol and nursery B has used the combination of penicillin G sodium and kanamycin sulfate routinely in the treatment of neonatal sepsis and other bacterial infections. A hypothesis was formulated that these different antibiotic pressures would select out a substantial number of populations of resistant bacteria in each of the two nurseries. This was tested by periodic sampling of the skin, mouth, and rectal flora of babies and the permanent personnel in these nurseries. These bacteria were studied for susceptibility to a number of antibiotics. The population of resistant strains selected out was correlated with the antibiotics used in each nursery. There is a need for continuing surveillance of hospital nursery strains of bacteria for in vitro susceptibilities to commonly prescribed antimicrobials."}
{"id": "PMID:1092153", "title": "Management of acute epiglottitis by nasotracheal intubation.", "content": "In 40 cases of acute epiglottitis in children, intubation was the chosen method for the management of airway obstruction. Six patients were treated without the establishment of an artificial airway, and no tracheostomies were done. No patients who were admitted to the hospital died of airway obstruction, although one sustained irreversible brain damage before admission, and two died of overwhelming infectionmthe average duration of intubation was 2.days and the average hospital stay was 5.days. Two children developed subglottic granulation tissue that was removed successfully and did not recur. Nasotracheal intubation is an acceptable method of management of epiglottitis.", "contents": "Management of acute epiglottitis by nasotracheal intubation. In 40 cases of acute epiglottitis in children, intubation was the chosen method for the management of airway obstruction. Six patients were treated without the establishment of an artificial airway, and no tracheostomies were done. No patients who were admitted to the hospital died of airway obstruction, although one sustained irreversible brain damage before admission, and two died of overwhelming infectionmthe average duration of intubation was 2.days and the average hospital stay was 5.days. Two children developed subglottic granulation tissue that was removed successfully and did not recur. Nasotracheal intubation is an acceptable method of management of epiglottitis."}
{"id": "PMID:1092154", "title": "Outbreak of lymphocytic choriomeningitis virus infections in medical center personnel.", "content": "An outbreak of lymphocytic choriomeningitis (LCM) virus infections occurred in Medical Center personnel at the University of Rochester in 1972-1973. A total of 48 infections was discovered, 31 in staff of the radiation therapy area, 12 in the staff of the Vivarium facility, and 5 in other individuals who worked in the Medical Center. Twenty-one of the infections were associated with recent febrile illness with myalgia. Epidemiologic and virologic studies indicated that the source of the infection was Syrian hamsters which were used in tumor research; the tumor cell lines themselves were shown to be contaminated with LCM virus and probably represented the original source of contamination of the Rochester facility. Infection occurred not only through direct contact with infected animals but also from mere presence in the room where the animals were held. This outbreak emphasizes the need for restricting access to animal quarters and for surveillance of hamsters and tumor cell lines and indicate the necessity of separating animal facilities from patient-care facilities.", "contents": "Outbreak of lymphocytic choriomeningitis virus infections in medical center personnel. An outbreak of lymphocytic choriomeningitis (LCM) virus infections occurred in Medical Center personnel at the University of Rochester in 1972-1973. A total of 48 infections was discovered, 31 in staff of the radiation therapy area, 12 in the staff of the Vivarium facility, and 5 in other individuals who worked in the Medical Center. Twenty-one of the infections were associated with recent febrile illness with myalgia. Epidemiologic and virologic studies indicated that the source of the infection was Syrian hamsters which were used in tumor research; the tumor cell lines themselves were shown to be contaminated with LCM virus and probably represented the original source of contamination of the Rochester facility. Infection occurred not only through direct contact with infected animals but also from mere presence in the room where the animals were held. This outbreak emphasizes the need for restricting access to animal quarters and for surveillance of hamsters and tumor cell lines and indicate the necessity of separating animal facilities from patient-care facilities."}
{"id": "PMID:1092155", "title": "Shigellosis at sea: an outbreak aboard a passenger cruise ship.", "content": "Between June 23 and June 30, 1973, 90% of 650 passengers and at least 35% of 299 crew members experienced a diarrheal illness during a 7-day Caribbean cruise aboard a passenger cruise liner. Symptoms were consistent with shigellosis, and Shigella flexneri 6, Boyd 88 biotype, was isolated from rectal swabs taken from 8 to 35 ill passengers and 33 of 294 crew members. Epidemiologic evidence incriminated the ship's water, including ice, as the probable vehicle of transmission, and elevated coliform counts were found in potable water samples obtained aboard the vessel at the peak of the outbreak. Potential sources of contamination of the vessel's potable water supply were investigated, and improvements in the loading and chlorination of potable water were recommended.", "contents": "Shigellosis at sea: an outbreak aboard a passenger cruise ship. Between June 23 and June 30, 1973, 90% of 650 passengers and at least 35% of 299 crew members experienced a diarrheal illness during a 7-day Caribbean cruise aboard a passenger cruise liner. Symptoms were consistent with shigellosis, and Shigella flexneri 6, Boyd 88 biotype, was isolated from rectal swabs taken from 8 to 35 ill passengers and 33 of 294 crew members. Epidemiologic evidence incriminated the ship's water, including ice, as the probable vehicle of transmission, and elevated coliform counts were found in potable water samples obtained aboard the vessel at the peak of the outbreak. Potential sources of contamination of the vessel's potable water supply were investigated, and improvements in the loading and chlorination of potable water were recommended."}
{"id": "PMID:1092156", "title": "An epidemiologic study of renal failure. I. The need for maintenance hemodialysis.", "content": "A nationwide morbidity study of end-stage kidney disease in Israel revealed a mean annual incidence rate, in the two-year period 1965-1966, of 70 cases per million per year requiring maintenance hemodialysis in the age 15-59 Jewish population. Chronic glomerulonephritis (38%) chronic pyelonephritis (24.9%) and polycystic disease (8.1%) represented the three most common diagnostic entities. It is suggested that the load of new cases on available dialysis units will not increase indefinitely but that an equilibrium will be established between the intake of new candidates and the outflow due to deaths and transplantation.", "contents": "An epidemiologic study of renal failure. I. The need for maintenance hemodialysis. A nationwide morbidity study of end-stage kidney disease in Israel revealed a mean annual incidence rate, in the two-year period 1965-1966, of 70 cases per million per year requiring maintenance hemodialysis in the age 15-59 Jewish population. Chronic glomerulonephritis (38%) chronic pyelonephritis (24.9%) and polycystic disease (8.1%) represented the three most common diagnostic entities. It is suggested that the load of new cases on available dialysis units will not increase indefinitely but that an equilibrium will be established between the intake of new candidates and the outflow due to deaths and transplantation."}
{"id": "PMID:1092157", "title": "Waterborne shigellosis at a public school.", "content": "In November 1972 an outbreak of gastrointestinal illness occurred at a public school in Stockport, Iowa. One hundred ninety-four (72%) of 269 pupils and 14 (16%) of 23 staff members were affected. The etiologic agent was a strain of Shigella sonnei resistant to multiple antimicrobials. Waterborne transmission of shigellosis was documented epidemiologically and by isolation of the organism from the school water system. Ninety-seven (14%) of 698 of the students' household contacts developed diarrhea, and possible secondary cases also occurred in 3 (9%) of 32 household contacts of school staff.", "contents": "Waterborne shigellosis at a public school. In November 1972 an outbreak of gastrointestinal illness occurred at a public school in Stockport, Iowa. One hundred ninety-four (72%) of 269 pupils and 14 (16%) of 23 staff members were affected. The etiologic agent was a strain of Shigella sonnei resistant to multiple antimicrobials. Waterborne transmission of shigellosis was documented epidemiologically and by isolation of the organism from the school water system. Ninety-seven (14%) of 698 of the students' household contacts developed diarrhea, and possible secondary cases also occurred in 3 (9%) of 32 household contacts of school staff."}
{"id": "PMID:1092159", "title": "Chemotherapy of infections in cancer patients: a review.", "content": "The factors altering host-defense mechanisms and the nature and significance of the resultant infections in cancer patients by a wide variety of invasive organisms are described. The principle and method of selection of antimicrobial agents as therapy in initial empiric treatment of presumed infection in febrile cancer patients, as well as therapeutic agents fothe therapy of infections by specfic organisms, are discussed. Special emphasis is placed on the more recently developed agents for the therapy of infections by Pseudomonas and other gram-negative bacilli.", "contents": "Chemotherapy of infections in cancer patients: a review. The factors altering host-defense mechanisms and the nature and significance of the resultant infections in cancer patients by a wide variety of invasive organisms are described. The principle and method of selection of antimicrobial agents as therapy in initial empiric treatment of presumed infection in febrile cancer patients, as well as therapeutic agents fothe therapy of infections by specfic organisms, are discussed. Special emphasis is placed on the more recently developed agents for the therapy of infections by Pseudomonas and other gram-negative bacilli."}
{"id": "PMID:1092160", "title": "Digitalis pharmacokinetics and metabolism.", "content": "The pharmacokinetics of the cardiac glycofides have been elucidated as a result of the development of assays of sufficient sensitivity to measure the concentration of digitalis compounds in biological fluids. Digoxin can accumulate in the body without the administration of a loading dose, and a steady state blood concentration will be reached in 5 to 7 days. Digitoxin requires 35 days to accumulate to a plateau. If a loading dose of digoxin is used, it should be approximately three times the estimated daily maintenance dose. Factors that determine the selection of the appropriate maintenance dose of digoxin include renal function and lean body mass. Digitoxin is less dependent on renal function for its elimination than is digoxin. Knowledge of the pharmacokinetics of digitalis preparations is useful in determining how to change from one cardiac glycoside to another, each with different half-lives. One should wait 3 days before starting digoxin therapy when changing from maintenance digitoxin to digoxin (assuming normal renal function). The pharmacokinetics of changing from ouabain to digoxin without loss of digitalis effect are described. The metabolism of the commonly used digitalis preparations are summarized.", "contents": "Digitalis pharmacokinetics and metabolism. The pharmacokinetics of the cardiac glycofides have been elucidated as a result of the development of assays of sufficient sensitivity to measure the concentration of digitalis compounds in biological fluids. Digoxin can accumulate in the body without the administration of a loading dose, and a steady state blood concentration will be reached in 5 to 7 days. Digitoxin requires 35 days to accumulate to a plateau. If a loading dose of digoxin is used, it should be approximately three times the estimated daily maintenance dose. Factors that determine the selection of the appropriate maintenance dose of digoxin include renal function and lean body mass. Digitoxin is less dependent on renal function for its elimination than is digoxin. Knowledge of the pharmacokinetics of digitalis preparations is useful in determining how to change from one cardiac glycoside to another, each with different half-lives. One should wait 3 days before starting digoxin therapy when changing from maintenance digitoxin to digoxin (assuming normal renal function). The pharmacokinetics of changing from ouabain to digoxin without loss of digitalis effect are described. The metabolism of the commonly used digitalis preparations are summarized."}
{"id": "PMID:1092161", "title": "Serum digitalis measurements in the assessment of digitalis resistance and sensitivity.", "content": "Antibodies to digitalis glycosides have been elicited in experimental animals and have been utilized in the development of rapid, sensitive, specific and convenient radioimmunoassay methods for the clinical measurement of digoxin and other cardiac glycosides in man. The use of these assay methods has supplemented earlier studies with radiolabeled digitalis preparations and has made it possible to obtain much new information concerning factors which may contribute to the well known patient to patient variability in digitalis dosage requirements and in sensitivity to the toxic effects of cardiac glycosides. In some patients with a poor clinical response to digitalis, the finding of a serum concentration which is relatively low for the dose prescribed may suggest that true digitalis resistance is not present and may raise questions of poor patient compliance, tablet inadequacies, intestinal malabsorption, increased metabolic degradation or hyperthyroidism; if the cause of the low serum level cannot be identified or corrected, serial serum measurements should enable safe and rational upward adjustment of dosage. In some patients with digitalis toxicity, the finding of a serum level which is relativity high for the dose prescribed may suggest that the patient is not sensitive to digitalis but rather is excreting it slowly; in such instances in elderly patients (with decreased glomerular filtration rates) and in patients with renal disease, serial digitalis measurements are useful adjuncts to clinical observation in determining optimal digitalis dosage schedules. A knowledge of serum digitalis concentrations should enable us to develop sound principles for a more rational approach to the clinical administration of cardiac glycosides, especially in patients with unusually high dosage requirements or with unusual sensitivity to relatively small doses of digitalis.", "contents": "Serum digitalis measurements in the assessment of digitalis resistance and sensitivity. Antibodies to digitalis glycosides have been elicited in experimental animals and have been utilized in the development of rapid, sensitive, specific and convenient radioimmunoassay methods for the clinical measurement of digoxin and other cardiac glycosides in man. The use of these assay methods has supplemented earlier studies with radiolabeled digitalis preparations and has made it possible to obtain much new information concerning factors which may contribute to the well known patient to patient variability in digitalis dosage requirements and in sensitivity to the toxic effects of cardiac glycosides. In some patients with a poor clinical response to digitalis, the finding of a serum concentration which is relatively low for the dose prescribed may suggest that true digitalis resistance is not present and may raise questions of poor patient compliance, tablet inadequacies, intestinal malabsorption, increased metabolic degradation or hyperthyroidism; if the cause of the low serum level cannot be identified or corrected, serial serum measurements should enable safe and rational upward adjustment of dosage. In some patients with digitalis toxicity, the finding of a serum level which is relativity high for the dose prescribed may suggest that the patient is not sensitive to digitalis but rather is excreting it slowly; in such instances in elderly patients (with decreased glomerular filtration rates) and in patients with renal disease, serial digitalis measurements are useful adjuncts to clinical observation in determining optimal digitalis dosage schedules. A knowledge of serum digitalis concentrations should enable us to develop sound principles for a more rational approach to the clinical administration of cardiac glycosides, especially in patients with unusually high dosage requirements or with unusual sensitivity to relatively small doses of digitalis."}
{"id": "PMID:1092162", "title": "Digitalis toxicity: epidemiology and clinical use of serum concentration measurements.", "content": "Despite continuing advances in understanding of the basic pharmacology of the cardiac glycosides, digitalis intoxication remains a common clinical problem. Physician education programs and increasing use of serum or plasma concentration data have, however, been shown to be capable of substantially reducing the incidence of digitalis toxicity. Methodologic progress and availability of commercial radioimmunoassay kits have placed measurement of clinically relevant serum or plasma cardiac glycoside concentrations within the capability of most well equipped clinical laboratories. Extensive experience with serum digitalis levels now provides a basis for ongoing examination of the role of these measurements in clinical practice. Results of studies to date demonstrate that mean serum digoxin and digitoxin levels are significantly higher in patients with electrocardiographic evidence of toxicity compared with patients without such evidence. It must be emphasized, however, that because of overlap in serum digitalis levels between these two groups, sole dependence on these levels for established of a diagnosis of digitalis toxicity is not warranted. Multiple factors influence individual responses to cardiac glycosides, and serum concentration data must be interpreted in the over-all clinical context. Type and extent of underlying heart disease are important determinants of the clinical response to any given dose or concentration of cardiac glycoside. Knowledge of the serum digitalis concentration is likely to be helpful in the setting of suspected digitalis intoxication in the absence of an adequate history, or in the presence of fluctuating renal function, overt or suspected malabsorption, or uncertain bioavailability. More generally, such measurements may prove useful whenever an unanticipated response to digitalis is encountered, whether it be suspected toxicity or the absence of an expected therapeutic effect.", "contents": "Digitalis toxicity: epidemiology and clinical use of serum concentration measurements. Despite continuing advances in understanding of the basic pharmacology of the cardiac glycosides, digitalis intoxication remains a common clinical problem. Physician education programs and increasing use of serum or plasma concentration data have, however, been shown to be capable of substantially reducing the incidence of digitalis toxicity. Methodologic progress and availability of commercial radioimmunoassay kits have placed measurement of clinically relevant serum or plasma cardiac glycoside concentrations within the capability of most well equipped clinical laboratories. Extensive experience with serum digitalis levels now provides a basis for ongoing examination of the role of these measurements in clinical practice. Results of studies to date demonstrate that mean serum digoxin and digitoxin levels are significantly higher in patients with electrocardiographic evidence of toxicity compared with patients without such evidence. It must be emphasized, however, that because of overlap in serum digitalis levels between these two groups, sole dependence on these levels for established of a diagnosis of digitalis toxicity is not warranted. Multiple factors influence individual responses to cardiac glycosides, and serum concentration data must be interpreted in the over-all clinical context. Type and extent of underlying heart disease are important determinants of the clinical response to any given dose or concentration of cardiac glycoside. Knowledge of the serum digitalis concentration is likely to be helpful in the setting of suspected digitalis intoxication in the absence of an adequate history, or in the presence of fluctuating renal function, overt or suspected malabsorption, or uncertain bioavailability. More generally, such measurements may prove useful whenever an unanticipated response to digitalis is encountered, whether it be suspected toxicity or the absence of an expected therapeutic effect."}
{"id": "PMID:1092163", "title": "Pharmacologic and clinical control of antiarrhythmic drugs.", "content": "Knowledge of pharmacokinetics and pharmacodynamics is a powerful tool for controlling cardiac arrhythmias with drugs even though antiarrhythmic drugs are potentially quite toxic. If the diagnosis and drug selection are correct at the outset of therapy, the clinician can use his knowledge of pharmacokinetics to achieve arrhythmia control with a minimum of personal effort and risk to his patient.", "contents": "Pharmacologic and clinical control of antiarrhythmic drugs. Knowledge of pharmacokinetics and pharmacodynamics is a powerful tool for controlling cardiac arrhythmias with drugs even though antiarrhythmic drugs are potentially quite toxic. If the diagnosis and drug selection are correct at the outset of therapy, the clinician can use his knowledge of pharmacokinetics to achieve arrhythmia control with a minimum of personal effort and risk to his patient."}
{"id": "PMID:1092164", "title": "Effects of ascorbic acid on the common cold. An evaluation of the evidence.", "content": "Of 14 clinical trials of ascorbic acid in the prevention and treatment of the common cold, the data from 8 were considered well enough gathered to be creditable and to warrant combining for an over-all assessment of efficacy. Differences in mean prorated numbers of colds per year and durations of illness were 0.09 plus or minus 0.06 (plus or minus 1 standard error) and 0.11 plus or minus 0.24, respectively, favoring ascorbic acid over the placebo. These are minor and insignificant differences, but in most studies the severity of symptoms was significantly worse in the patients who received the placebo. In one study lasting 9 months, a large number of the volunteers tasted their capsules and correctly guessed what group they were in. All differences in severity and duration were eliminated by analyzing only the data from those who did not know which drug they were taking. Since there are no data on the long-term toxicity of ascorbic acid when given in doses of 1 g or more per day, it is concluded that the minor benefits of questionable validity are not worth the potential risk, no matter how small that might be.", "contents": "Effects of ascorbic acid on the common cold. An evaluation of the evidence. Of 14 clinical trials of ascorbic acid in the prevention and treatment of the common cold, the data from 8 were considered well enough gathered to be creditable and to warrant combining for an over-all assessment of efficacy. Differences in mean prorated numbers of colds per year and durations of illness were 0.09 plus or minus 0.06 (plus or minus 1 standard error) and 0.11 plus or minus 0.24, respectively, favoring ascorbic acid over the placebo. These are minor and insignificant differences, but in most studies the severity of symptoms was significantly worse in the patients who received the placebo. In one study lasting 9 months, a large number of the volunteers tasted their capsules and correctly guessed what group they were in. All differences in severity and duration were eliminated by analyzing only the data from those who did not know which drug they were taking. Since there are no data on the long-term toxicity of ascorbic acid when given in doses of 1 g or more per day, it is concluded that the minor benefits of questionable validity are not worth the potential risk, no matter how small that might be."}
{"id": "PMID:1092166", "title": "Persistent lymphocytosis with chromosomal evidence of malignancy.", "content": "An inappropriate, sustained and absolute lymphocytosis in a young man was investigated using technics which attempted to define the more detailed features of these cells and their T and B subpopulations. The results of the tests for lymphocyte RFC (E) and complement (EAC) rosette formation, immunofluorescence, in vitro phytohemagglutin (PHA) stimulation and 14C-cyclo-phosphamide binding assays, when evaluated in a combined and interrelated fashion, indicated that the lymphocytosis was mainly but not solely, due to an absolute elevation in circulating T lymphocytes. Moreover, an aneuploid cell line with 47 chromosomes, presumably T cells, also was detected in PHA-stimulated cultures. These data, associated with the patient's anemia, lymphocyte-infiltrated bone marrow, hepatosplenomegaly, pharyngeal lymphoid tumor and the clonal proliferation of lymphocytes bearing an abnormal karyotype, furnish evidence that the lymphocytosis is more than an unremitting leukemoid reaction and is potentially neoplastic. The detection of the chromosomal abnormality at this patient's age and point in disease may make him especially unique in that he could be a clinical prototype for other patients with lymphoreticular tumors which remain undetected at their onset.", "contents": "Persistent lymphocytosis with chromosomal evidence of malignancy. An inappropriate, sustained and absolute lymphocytosis in a young man was investigated using technics which attempted to define the more detailed features of these cells and their T and B subpopulations. The results of the tests for lymphocyte RFC (E) and complement (EAC) rosette formation, immunofluorescence, in vitro phytohemagglutin (PHA) stimulation and 14C-cyclo-phosphamide binding assays, when evaluated in a combined and interrelated fashion, indicated that the lymphocytosis was mainly but not solely, due to an absolute elevation in circulating T lymphocytes. Moreover, an aneuploid cell line with 47 chromosomes, presumably T cells, also was detected in PHA-stimulated cultures. These data, associated with the patient's anemia, lymphocyte-infiltrated bone marrow, hepatosplenomegaly, pharyngeal lymphoid tumor and the clonal proliferation of lymphocytes bearing an abnormal karyotype, furnish evidence that the lymphocytosis is more than an unremitting leukemoid reaction and is potentially neoplastic. The detection of the chromosomal abnormality at this patient's age and point in disease may make him especially unique in that he could be a clinical prototype for other patients with lymphoreticular tumors which remain undetected at their onset."}
{"id": "PMID:1092170", "title": "In vitro studies on human cervical epithelium, benign and neoplastic.", "content": "The early neoplastic lesions of the cervix offer an excellent model to study the neoplastic process in the human; In vitro studies indicate that these lesions are intermediate between normal epithelium and invasive carcinoma in many aspects. More recent studies show that the in vitro cells retain many of the characteristics of their in vivo counterparts. The techniques are available for short-term culture of normal and CIN cells, but no cell lines have been established. Spontaneous transformation of normal cervical epithelial cells in culture has been rare, but planned transformation with chemical carcinogens and HSV-2 has not been successful. Even though much preliminary work has been done, the establishment of a cell line from the human cervix and methods for transforming normal cell in vitro are needed. Studies toward these objectives are continuing in this laboratory.", "contents": "In vitro studies on human cervical epithelium, benign and neoplastic. The early neoplastic lesions of the cervix offer an excellent model to study the neoplastic process in the human; In vitro studies indicate that these lesions are intermediate between normal epithelium and invasive carcinoma in many aspects. More recent studies show that the in vitro cells retain many of the characteristics of their in vivo counterparts. The techniques are available for short-term culture of normal and CIN cells, but no cell lines have been established. Spontaneous transformation of normal cervical epithelial cells in culture has been rare, but planned transformation with chemical carcinogens and HSV-2 has not been successful. Even though much preliminary work has been done, the establishment of a cell line from the human cervix and methods for transforming normal cell in vitro are needed. Studies toward these objectives are continuing in this laboratory."}
{"id": "PMID:1092171", "title": "Histologic and nuclear grading and stromal reactions as indices for prognosis in ovarian cancer.", "content": "Ovarian cancer is one of the most frustrating problems in gynecology. Each year about 14,000 new cases are diagnosed in the United States and almost 11,000 patients die from their ovarian cancer. The results from treatment are no better in 1973 than they were in the previous two decades; Early diagnosis is a matter of chance rather than a scientific method. Until early diagnosis can be made and treatment instituted with predictable promise for cure, attention must be directed to study the natural history of disease, its histologic and nuclear grading, as well as the stromal reaction of the cancer. This approach should further validate end results and, in the future, help improve the survival rate in ovarian carcinoma. With these criteria, 174 fresh cases were evaluated. The study showed that undifferentiated cancers were mainly in the unfavorable histologic and nuclear grades, while the better differentiated tumors were in the more favorable group. Mucinous cancers were characterized by the greatest number of tumors with favorable histologic and nuclear grades and generally had a better survival rate. Since the potency of tumors vary within a given stage, treatment should be tailored to the cancer rather than the stage of disease; In general, stage of disease was more important than histologic or nuclear grades in determining prognosis.", "contents": "Histologic and nuclear grading and stromal reactions as indices for prognosis in ovarian cancer. Ovarian cancer is one of the most frustrating problems in gynecology. Each year about 14,000 new cases are diagnosed in the United States and almost 11,000 patients die from their ovarian cancer. The results from treatment are no better in 1973 than they were in the previous two decades; Early diagnosis is a matter of chance rather than a scientific method. Until early diagnosis can be made and treatment instituted with predictable promise for cure, attention must be directed to study the natural history of disease, its histologic and nuclear grading, as well as the stromal reaction of the cancer. This approach should further validate end results and, in the future, help improve the survival rate in ovarian carcinoma. With these criteria, 174 fresh cases were evaluated. The study showed that undifferentiated cancers were mainly in the unfavorable histologic and nuclear grades, while the better differentiated tumors were in the more favorable group. Mucinous cancers were characterized by the greatest number of tumors with favorable histologic and nuclear grades and generally had a better survival rate. Since the potency of tumors vary within a given stage, treatment should be tailored to the cancer rather than the stage of disease; In general, stage of disease was more important than histologic or nuclear grades in determining prognosis."}
{"id": "PMID:1092172", "title": "Ocular clinical findings and basement membrane changes in Goodpasture's syndrome.", "content": "Goodpasture's syndrome is a rapidly progressive disease characterized by the onset of pulmonary hemorrhage with glomerulonephritis, leading to pulmonary and renal failure. The hallmark of this disease is linear deposition of antibasement membrane IgG in the basement membranes of the kidney and lung. In two cases of nonrhegmatogenous retinal detachment in patients with Goodpasture's syndrome, the first patient demonstrated multiple ischemic areas in the choroid on fluorescein angiography. Histopathologic and immunologic studies of the eyes in the second patient demonstrated macular edema and elevation of the retina in association with choroidal infarction and linear deposition of IgG in Burch's membrane and the basement membranes of the choroidal vessels.", "contents": "Ocular clinical findings and basement membrane changes in Goodpasture's syndrome. Goodpasture's syndrome is a rapidly progressive disease characterized by the onset of pulmonary hemorrhage with glomerulonephritis, leading to pulmonary and renal failure. The hallmark of this disease is linear deposition of antibasement membrane IgG in the basement membranes of the kidney and lung. In two cases of nonrhegmatogenous retinal detachment in patients with Goodpasture's syndrome, the first patient demonstrated multiple ischemic areas in the choroid on fluorescein angiography. Histopathologic and immunologic studies of the eyes in the second patient demonstrated macular edema and elevation of the retina in association with choroidal infarction and linear deposition of IgG in Burch's membrane and the basement membranes of the choroidal vessels."}
{"id": "PMID:1092173", "title": "Pseudorheumatoid nodules of the ocular adnexa.", "content": "A clinicopathologic study of 21 cases revealed pseudorheumatoid subcutaneous nodules occurring in the eyelids and periorbital region of children and young adults. These nodules were most frequently located in the lateral upper eyelids and outer canthal region, and in five cases they were multiple. The lesions recurred in about 20% of the cases. In addition, new lesions located elsewhere were observed in 26% of cases. Histopathologically they resembled the subcutaneous nodules of rheumatoid arthritis and rheumatic fever. Follow-up information on 15 patients (from two to 12 years) and the results of laboratory studies on seven patients had failed to reveal any association with these two systemic diseases. Pseudorheumatoid nodules may be indicated when a healthy child or a young adult presents with solitary or multiple subcutaneous nodules, especially in the eyelids. These lesions pursued a benign clinical course and local excision, which is usually performed for diagnostic purposes, was the only treatment indicated. The lesions had a tendency to regress spontaneously; therefore, local recurrences should be treated conservatively by periodic follow-up examinations.", "contents": "Pseudorheumatoid nodules of the ocular adnexa. A clinicopathologic study of 21 cases revealed pseudorheumatoid subcutaneous nodules occurring in the eyelids and periorbital region of children and young adults. These nodules were most frequently located in the lateral upper eyelids and outer canthal region, and in five cases they were multiple. The lesions recurred in about 20% of the cases. In addition, new lesions located elsewhere were observed in 26% of cases. Histopathologically they resembled the subcutaneous nodules of rheumatoid arthritis and rheumatic fever. Follow-up information on 15 patients (from two to 12 years) and the results of laboratory studies on seven patients had failed to reveal any association with these two systemic diseases. Pseudorheumatoid nodules may be indicated when a healthy child or a young adult presents with solitary or multiple subcutaneous nodules, especially in the eyelids. These lesions pursued a benign clinical course and local excision, which is usually performed for diagnostic purposes, was the only treatment indicated. The lesions had a tendency to regress spontaneously; therefore, local recurrences should be treated conservatively by periodic follow-up examinations."}
{"id": "PMID:1092175", "title": "ABO Blood groups and corneal transplantation.", "content": "A study of 150 corneal donor-recipient grafts, examined for ABO type, revealed that 25% of the grafts were from corneas from incompatible donors. Recipients with histocompatible sensitization exposures such as prior corneal transplant, one or more pregnancies, or one or more blood transfusions were no more likely to experience graft failure if the graft were from an ABO incompatible donor than from a compatible donor. Of 13 graft failures (8%), three of the failed grafts were from AB donors, a significant result. No detrimental effect of transplanting across the ABO barrier could be proven with assurance for any parameter.", "contents": "ABO Blood groups and corneal transplantation. A study of 150 corneal donor-recipient grafts, examined for ABO type, revealed that 25% of the grafts were from corneas from incompatible donors. Recipients with histocompatible sensitization exposures such as prior corneal transplant, one or more pregnancies, or one or more blood transfusions were no more likely to experience graft failure if the graft were from an ABO incompatible donor than from a compatible donor. Of 13 graft failures (8%), three of the failed grafts were from AB donors, a significant result. No detrimental effect of transplanting across the ABO barrier could be proven with assurance for any parameter."}
{"id": "PMID:1092174", "title": "Cyclocryotherapy for glaucoma after penetrating keratoplasty.", "content": "Thirty-six eyes underwent cyclocryotherapy after penetrating keratoplasty for medically unmanageable elevated intraocular pressures. Thirty were controlled with one procedure, five required three procedures. Eighty-two percent of the transplants remained clear after cryotherapy and 19 of 23 with clear grafts obtained useful visual acuity. Our results including the number of patients whose glaucoma was controlled by this procedure and the percent of clear grafts following cryotherapy are far better than results of other forms of glaucoma surgery after keratoplasty. The complication rate was 14% for all procedures. Most procedures were performed within 12 weeks of the corneal transplant. Cyclocryotherapy is necessary for the successful management of glaucoma after penetrating keratoplasty to avoid graft failure and must be performed if acceptable visual acuity is to be attained.", "contents": "Cyclocryotherapy for glaucoma after penetrating keratoplasty. Thirty-six eyes underwent cyclocryotherapy after penetrating keratoplasty for medically unmanageable elevated intraocular pressures. Thirty were controlled with one procedure, five required three procedures. Eighty-two percent of the transplants remained clear after cryotherapy and 19 of 23 with clear grafts obtained useful visual acuity. Our results including the number of patients whose glaucoma was controlled by this procedure and the percent of clear grafts following cryotherapy are far better than results of other forms of glaucoma surgery after keratoplasty. The complication rate was 14% for all procedures. Most procedures were performed within 12 weeks of the corneal transplant. Cyclocryotherapy is necessary for the successful management of glaucoma after penetrating keratoplasty to avoid graft failure and must be performed if acceptable visual acuity is to be attained."}
{"id": "PMID:1092176", "title": "Endogenous Aspergillus endophthalmitis occurring after kidney transplant.", "content": "Two months after renal transplantation, a 26-year-old man developed pneumonia that was recalcitrant to antibiotic therapy and proved by biopsy to be due to cytomegalovirus and Aspergillus fumigatus. Ten days later while on amphotericin B therapy, he developed an endophthalmitis proved by smear and culture of a vitreous aspiration to be caused by A. fumigatus. Despite intravitreous and systemic amphotericin B the vision deteriorated and the eye was enucleated. Microscopic examination disclosed an intense endophthalmitis with vitreous and retinal abscesses. The second patient was a 29-year-old woman who developed severe hypertension and graft rejection one month after renal transplant, despite massive immunosuppressive therapy with prednisone, azathioprine, and cobalt 60 irradiation. She developed pneumonia, meningitis, and died. A postmortem examination revealed disseminated aspergillosis. A single choroidal abscess due to Aspergillus with an associated retinal hemorrhage was observed in the left eye.", "contents": "Endogenous Aspergillus endophthalmitis occurring after kidney transplant. Two months after renal transplantation, a 26-year-old man developed pneumonia that was recalcitrant to antibiotic therapy and proved by biopsy to be due to cytomegalovirus and Aspergillus fumigatus. Ten days later while on amphotericin B therapy, he developed an endophthalmitis proved by smear and culture of a vitreous aspiration to be caused by A. fumigatus. Despite intravitreous and systemic amphotericin B the vision deteriorated and the eye was enucleated. Microscopic examination disclosed an intense endophthalmitis with vitreous and retinal abscesses. The second patient was a 29-year-old woman who developed severe hypertension and graft rejection one month after renal transplant, despite massive immunosuppressive therapy with prednisone, azathioprine, and cobalt 60 irradiation. She developed pneumonia, meningitis, and died. A postmortem examination revealed disseminated aspergillosis. A single choroidal abscess due to Aspergillus with an associated retinal hemorrhage was observed in the left eye."}
{"id": "PMID:1092187", "title": "The pulmonary inflammatory response. Cellular events in experimental pulmonary arterial hypersensitivity disease.", "content": "Horseradish peroxidase (HRP) or bovine serum albumin (BSA) were covalently linked to polyacrylamide or agarose beads and were injected into control Syrian hamsters and hamsters previously immunized with either HRP or BSA. Animals sensitized to soluble antigen and subsequently challenged intravenously with the same antigen immobilized on beads developed an acute focal inflammatory response within 2 to 6 hours after injection. The acute response involved local deposition of IgG and complement (beta1A/beta1C globulin), polymorphonuclear leukocyte exudation, and variable amounts of hemorrhage. A focal vasculitis was occasionally present. Within 72 hours the reaction had become largely mononuclear or granulomatous in nature, and giant cell formation was seen within 4 days after immobilized antigen injection. Severe reactions developed only upon recognition of specific antigenic determinants; thus hamsters immunized against soluble HRP developed characteristic lesions only upon intravenous challenge with HRP-coated beads but not with beads coated with unrelated antigen (BSA). The beads elicited only a mild foreign body reaction in the control hamsters at 5 to 7 days after injection which was temporally and histopathologically distinct from the lesions in immunized hamsters. Thus, the state of existing immunity can influence the character and severity of the local pulmonary inflammatory response.", "contents": "The pulmonary inflammatory response. Cellular events in experimental pulmonary arterial hypersensitivity disease. Horseradish peroxidase (HRP) or bovine serum albumin (BSA) were covalently linked to polyacrylamide or agarose beads and were injected into control Syrian hamsters and hamsters previously immunized with either HRP or BSA. Animals sensitized to soluble antigen and subsequently challenged intravenously with the same antigen immobilized on beads developed an acute focal inflammatory response within 2 to 6 hours after injection. The acute response involved local deposition of IgG and complement (beta1A/beta1C globulin), polymorphonuclear leukocyte exudation, and variable amounts of hemorrhage. A focal vasculitis was occasionally present. Within 72 hours the reaction had become largely mononuclear or granulomatous in nature, and giant cell formation was seen within 4 days after immobilized antigen injection. Severe reactions developed only upon recognition of specific antigenic determinants; thus hamsters immunized against soluble HRP developed characteristic lesions only upon intravenous challenge with HRP-coated beads but not with beads coated with unrelated antigen (BSA). The beads elicited only a mild foreign body reaction in the control hamsters at 5 to 7 days after injection which was temporally and histopathologically distinct from the lesions in immunized hamsters. Thus, the state of existing immunity can influence the character and severity of the local pulmonary inflammatory response."}
{"id": "PMID:1092189", "title": "A primate model of hyperacute renal allograft rejection.", "content": "Hyperacute renal allograft rejection is initiated by primary immune injury to vascular endothelium and is propagated by secondary vasoconstriction, platelet aggregation and intravascular coagulation. Previous dissociation of these primary and secondary events, with graft survival in one human, suggested that the more usual graft failure was due to secondary injury. As a basis for further modification studies, this primate model most closely resembled its counterpart in man, as the onset and intensity of functional, morphologic and biochemical alterations were similar. Unmodified allografts failed within 5 minutes. The earliest and most abnormal finding was marked reduction in renal blood flow affecting all compartments. By 5 minutes, histologic changes of hyperacute rejection as well as antibody and faint C3 deposits were noted, but biopsies suggested that the initial flow reduction was more likely due to vasoconstriction, which was then followed by vascular obstruction. Glomeruli appeared most damaged, but at the highest antibody titer arterial injury was more prominent. Early red cell sequestration and stasis was marked, followed by progressive platelet clumping and neutrophil infiltration. While the decline in renal venous C3 levels was prompt, as in man, early intrarenal activation of the coagulation, fibrinolytic and kinin-forming systems could not be demonstrated, and fibrin formation was sparse by light and fluorescence microscopy. Qualitatively similar histologic and functional alterations were noted in autograft controls. While the initiating event was unclear and may have been accentuated by the arteriovenous shunts utilized, the final mechanism was probably marked vasoconstriction with renal ischemia. Intrarenal C3 consumption was an important finding and was not associated with tissue deposits of antibody or complement; it may provide a parallel with the progressive complement-mediated injury associated with acute myocardial ischemia noted by others. Endothelial injury was not seen in arteries, and all alterations were delayed in onset and progressed more slowly than in allografts. These findings may elucidate the mechanism of early malfunction of most autografts. Treatment of additional autografts with increasing doses of heparin progressively reversed these changes and even prevented the initial reduction in blood flow. Therefore, many alterations consistent with hyperacute rejection which are probably immediately responsible for graft failure can also be initiated by nonspecific, nonimmunologic events and, where injury is less intense, can be prevented pharmacologically. This model provides a means of dissecting the injurious events and subsequent evaluation of the effectiveness and interaction of various agents on the damaging secondary alterations which occur during hyperacute rejection.", "contents": "A primate model of hyperacute renal allograft rejection. Hyperacute renal allograft rejection is initiated by primary immune injury to vascular endothelium and is propagated by secondary vasoconstriction, platelet aggregation and intravascular coagulation. Previous dissociation of these primary and secondary events, with graft survival in one human, suggested that the more usual graft failure was due to secondary injury. As a basis for further modification studies, this primate model most closely resembled its counterpart in man, as the onset and intensity of functional, morphologic and biochemical alterations were similar. Unmodified allografts failed within 5 minutes. The earliest and most abnormal finding was marked reduction in renal blood flow affecting all compartments. By 5 minutes, histologic changes of hyperacute rejection as well as antibody and faint C3 deposits were noted, but biopsies suggested that the initial flow reduction was more likely due to vasoconstriction, which was then followed by vascular obstruction. Glomeruli appeared most damaged, but at the highest antibody titer arterial injury was more prominent. Early red cell sequestration and stasis was marked, followed by progressive platelet clumping and neutrophil infiltration. While the decline in renal venous C3 levels was prompt, as in man, early intrarenal activation of the coagulation, fibrinolytic and kinin-forming systems could not be demonstrated, and fibrin formation was sparse by light and fluorescence microscopy. Qualitatively similar histologic and functional alterations were noted in autograft controls. While the initiating event was unclear and may have been accentuated by the arteriovenous shunts utilized, the final mechanism was probably marked vasoconstriction with renal ischemia. Intrarenal C3 consumption was an important finding and was not associated with tissue deposits of antibody or complement; it may provide a parallel with the progressive complement-mediated injury associated with acute myocardial ischemia noted by others. Endothelial injury was not seen in arteries, and all alterations were delayed in onset and progressed more slowly than in allografts. These findings may elucidate the mechanism of early malfunction of most autografts. Treatment of additional autografts with increasing doses of heparin progressively reversed these changes and even prevented the initial reduction in blood flow. Therefore, many alterations consistent with hyperacute rejection which are probably immediately responsible for graft failure can also be initiated by nonspecific, nonimmunologic events and, where injury is less intense, can be prevented pharmacologically. This model provides a means of dissecting the injurious events and subsequent evaluation of the effectiveness and interaction of various agents on the damaging secondary alterations which occur during hyperacute rejection."}
{"id": "PMID:1092190", "title": "Medical practice without antianxiety drugs.", "content": "The benzodiazepines and hydroxyzine were removed from a statewide Medicaid pharmacopeia. Barbiturate sedatives, hypnotic and antipsychotic medication, and tricyclic antidepressants remained available, but no substitution was made for about two-thirds of the deleted ataractic medications. The authors conclide that most physicans regard ataractics as unique and do not use them interchangeably with the other drugs.", "contents": "Medical practice without antianxiety drugs. The benzodiazepines and hydroxyzine were removed from a statewide Medicaid pharmacopeia. Barbiturate sedatives, hypnotic and antipsychotic medication, and tricyclic antidepressants remained available, but no substitution was made for about two-thirds of the deleted ataractic medications. The authors conclide that most physicans regard ataractics as unique and do not use them interchangeably with the other drugs."}
{"id": "PMID:1092194", "title": "Reoperation after esophagomyotomy for achalasia of the esophagus.", "content": "Eleven patients who required reoperation for persistent or recurrent symptoms after esophagomyotomy are reported on. Failure of the original operation was attributed to inadequate myotomy in three, healed myotomy in four, and reflux esophagitis in four. The myotomy was extended or a new myotomy created in the first two categories of patients. Three of the four patients with reflux esophagitis were treated by antrectomy and Roux-en-Y esophagojejunostomy; one of these also required concomitant exision of the esophagogastric junction because of stricture. The fourth patient with esophagitis ultimately required colonic interposition for relief of symptoms. Ten of the eleven patients were improved after reoperation, but results were less good than those achieved by a properly performed primary esophagomyotomy. This -re-emphasizes the need for attention to the technical details of esophagomyotomy if good results are to be achieved.", "contents": "Reoperation after esophagomyotomy for achalasia of the esophagus. Eleven patients who required reoperation for persistent or recurrent symptoms after esophagomyotomy are reported on. Failure of the original operation was attributed to inadequate myotomy in three, healed myotomy in four, and reflux esophagitis in four. The myotomy was extended or a new myotomy created in the first two categories of patients. Three of the four patients with reflux esophagitis were treated by antrectomy and Roux-en-Y esophagojejunostomy; one of these also required concomitant exision of the esophagogastric junction because of stricture. The fourth patient with esophagitis ultimately required colonic interposition for relief of symptoms. Ten of the eleven patients were improved after reoperation, but results were less good than those achieved by a properly performed primary esophagomyotomy. This -re-emphasizes the need for attention to the technical details of esophagomyotomy if good results are to be achieved."}
{"id": "PMID:1092195", "title": "Clinical applications of thermodilution cardiac output determinations.", "content": "The pulmonary arterial catheter was shown to be safe and easily positioned in a group of 200 patients. Thermodilution cardiac output measurement was shown to be simple, rapid, and safe. The technic was as accurate as the simultaneously measured indocyanine green cardiac output, and more reproducible, particularly at high and low flows. The construction of myocardial performance curves during a fluid challenge is described. The value of these curves is guiding fluid, drug, and respirator therapy is illustrated by case presentations.", "contents": "Clinical applications of thermodilution cardiac output determinations. The pulmonary arterial catheter was shown to be safe and easily positioned in a group of 200 patients. Thermodilution cardiac output measurement was shown to be simple, rapid, and safe. The technic was as accurate as the simultaneously measured indocyanine green cardiac output, and more reproducible, particularly at high and low flows. The construction of myocardial performance curves during a fluid challenge is described. The value of these curves is guiding fluid, drug, and respirator therapy is illustrated by case presentations."}
{"id": "PMID:1092197", "title": "[Experimental erysipelas in different species as a model for systemic connective tissue disease. I. Systemic vascular processes during organ manifestation (author's transl)].", "content": "The similarities between erysipelas in animals and rheumatic diseases in man have been discussed since the work of Nieberle (1931). The present work sets out to investigate the course of organ manifestations in pigs, rats, and mice using germ-free or specific pathogen-free experimental animals. Particular consideration will be given to the initial systemic vascular processes as well as to the significance of the erysipelas antigen. In several experiments, a total of 166 pigs--partly gnotobiotic or specific pathogen-free animals--37 specific pathogen free Wistar rats and 57 albino mice were orally and/or parenterally infected with standardized erysipelas strains of serotype B. Clinical examination post infection were carried out with the EKG and by x-raying the joints of the extremities. All large parenchymatous organs, as well as heart valves, aorta and synovia were examined histologically in paraffin sections. In mice and rats, joints of the extremities were embedded in toto in metracrylate. Besides various histological staining methods, histochemical reactions were used to demonstrate mucopolysaccharides and fibrin. The myocardium, central nervous system and synovia of several joints were examined with the electron microscope. In the pig, immunohistological methods demonstrating the presence of fibrin, complement and IgG, as described by Seidler et al. (1971) and Trautwein et al. (1972), were used. The most important changes in joints, heart valves heart musculature and blood vessels occur during the early bacteriemic phase. A distinct sticking effect develops in the mouse 3.5 hours p.i., in the rat 24 hours p.i., and in the pig 36 hours p.i. Simultaneously, hyaline thrombi occur in capillaries and venules; these are seen as parallel, loosely-packed fibrin fibers in the electron microscope. With the aid of immunofluorescence fibrin, IgG and complement C3 can also be demonstrated here. Exudates rich in fibrin develop parallel to the microthrombosis. In pigs and rats vascular and myocardial necroses develop to 3  days p.i. The mice do not survive the 3rd p.i. 39% of the pigs showed edema and mesenchymal activation of varying intensity in the heart valves between the 3rd and 8th day p.i. Besides the insudation of the valves, endocardial thromboses developed in 80% of the mice. Endocarditis, aand in addition large aortic thromboses were recognized in more than 50% of the rats. As early as the 4th day p.i., coagulopathy, angionecrosis and exudation led to acute arthritic symptoms..", "contents": "[Experimental erysipelas in different species as a model for systemic connective tissue disease. I. Systemic vascular processes during organ manifestation (author's transl)]. The similarities between erysipelas in animals and rheumatic diseases in man have been discussed since the work of Nieberle (1931). The present work sets out to investigate the course of organ manifestations in pigs, rats, and mice using germ-free or specific pathogen-free experimental animals. Particular consideration will be given to the initial systemic vascular processes as well as to the significance of the erysipelas antigen. In several experiments, a total of 166 pigs--partly gnotobiotic or specific pathogen-free animals--37 specific pathogen free Wistar rats and 57 albino mice were orally and/or parenterally infected with standardized erysipelas strains of serotype B. Clinical examination post infection were carried out with the EKG and by x-raying the joints of the extremities. All large parenchymatous organs, as well as heart valves, aorta and synovia were examined histologically in paraffin sections. In mice and rats, joints of the extremities were embedded in toto in metracrylate. Besides various histological staining methods, histochemical reactions were used to demonstrate mucopolysaccharides and fibrin. The myocardium, central nervous system and synovia of several joints were examined with the electron microscope. In the pig, immunohistological methods demonstrating the presence of fibrin, complement and IgG, as described by Seidler et al. (1971) and Trautwein et al. (1972), were used. The most important changes in joints, heart valves heart musculature and blood vessels occur during the early bacteriemic phase. A distinct sticking effect develops in the mouse 3.5 hours p.i., in the rat 24 hours p.i., and in the pig 36 hours p.i. Simultaneously, hyaline thrombi occur in capillaries and venules; these are seen as parallel, loosely-packed fibrin fibers in the electron microscope. With the aid of immunofluorescence fibrin, IgG and complement C3 can also be demonstrated here. Exudates rich in fibrin develop parallel to the microthrombosis. In pigs and rats vascular and myocardial necroses develop to 3  days p.i. The mice do not survive the 3rd p.i. 39% of the pigs showed edema and mesenchymal activation of varying intensity in the heart valves between the 3rd and 8th day p.i. Besides the insudation of the valves, endocardial thromboses developed in 80% of the mice. Endocarditis, aand in addition large aortic thromboses were recognized in more than 50% of the rats. As early as the 4th day p.i., coagulopathy, angionecrosis and exudation led to acute arthritic symptoms.."}
{"id": "PMID:1092198", "title": "[Corticosteroid cataracts following kidney transplantation. Investigations on the influence of additional factors upon the development of opacities (author's transl)].", "content": "15 patients with kidney transplants were followed up for a period of 3 to 45 months. All but one developed lenticular opacities in the posterior subsapsular region. The opacities were classified according to their severity. It could be shown that the cataract index was correlated to the total amount of corticosteroids given. There is probably an additional effect of age and azathioprin therapy. Possibly, the time of treatment with intermittent hemodialysis also influences cataract development.", "contents": "[Corticosteroid cataracts following kidney transplantation. Investigations on the influence of additional factors upon the development of opacities (author's transl)]. 15 patients with kidney transplants were followed up for a period of 3 to 45 months. All but one developed lenticular opacities in the posterior subsapsular region. The opacities were classified according to their severity. It could be shown that the cataract index was correlated to the total amount of corticosteroids given. There is probably an additional effect of age and azathioprin therapy. Possibly, the time of treatment with intermittent hemodialysis also influences cataract development."}
{"id": "PMID:1092199", "title": "[Clinical studies on the narcotic effects of nitrous oxide (author's transl)].", "content": "In 340 male and 450 female patients of various age the depth of anaesthesia acquired during induction with nitrous oxide (75%) and oxygen (25%) was investigated by means of clinical methods. Premedication consisted of atropine (0,1 mg/1o kg body weight) in 26% and atropine plus pethidine (or morphine) in 74% of the patients, respectively of the total, 2.4% of the patients failed to enter the analgesic-amnestic level, determined as I-3, while 97.6% occupied and more than 50% of the patients surpassed it. Thus the MAC of nitrous oxide in man seems to be 75 volume per cent or less. The depth of anaesthesia varied significantly with duration of inhalation (p smaller than 0.001). Up to five minutes of inhalation 5% of the patients failed to arrive at I-3, while 95% reached this plane and 46% surpassed it. After more than five minutes of inhalation the corresponding values were 1%, 99% and 56%, respectively. Significantly deeper levels of anaesthesia were acquired by females than by males (p smaller than 0.001) during the initial 10 minutes of inhalation. After that time sexual differences disappeared. Patients of good physical condition reached deeper planes of anaesthesia than bad risk cases (p smaller than 0.001). The use of analgesics as premedicants reduced the effectiveness of nitrous oxide, as compared to premedicated patients with atropine alone (p smaller than 0.01 in men and p smaller than 0.05 in women). Adults below 30 years of age entered the stages II and III-1 significantly less frequently than older patients (p smaller than 0.01), whereas children (up to 14 years of age) reached those stages significantly more frequently than adult patients in general (p smaller than 0.01).", "contents": "[Clinical studies on the narcotic effects of nitrous oxide (author's transl)]. In 340 male and 450 female patients of various age the depth of anaesthesia acquired during induction with nitrous oxide (75%) and oxygen (25%) was investigated by means of clinical methods. Premedication consisted of atropine (0,1 mg/1o kg body weight) in 26% and atropine plus pethidine (or morphine) in 74% of the patients, respectively of the total, 2.4% of the patients failed to enter the analgesic-amnestic level, determined as I-3, while 97.6% occupied and more than 50% of the patients surpassed it. Thus the MAC of nitrous oxide in man seems to be 75 volume per cent or less. The depth of anaesthesia varied significantly with duration of inhalation (p smaller than 0.001). Up to five minutes of inhalation 5% of the patients failed to arrive at I-3, while 95% reached this plane and 46% surpassed it. After more than five minutes of inhalation the corresponding values were 1%, 99% and 56%, respectively. Significantly deeper levels of anaesthesia were acquired by females than by males (p smaller than 0.001) during the initial 10 minutes of inhalation. After that time sexual differences disappeared. Patients of good physical condition reached deeper planes of anaesthesia than bad risk cases (p smaller than 0.001). The use of analgesics as premedicants reduced the effectiveness of nitrous oxide, as compared to premedicated patients with atropine alone (p smaller than 0.01 in men and p smaller than 0.05 in women). Adults below 30 years of age entered the stages II and III-1 significantly less frequently than older patients (p smaller than 0.01), whereas children (up to 14 years of age) reached those stages significantly more frequently than adult patients in general (p smaller than 0.01)."}
{"id": "PMID:1092205", "title": "Attention, learning, and personality during ketamine emergence: a pilot study.", "content": "To analyze the memory, learning, attention, and personality effects associated with emergence from partial ketamine induction, 11 resident physicians were tested. Ketamine was found to be truly dissociative in that it seemed to impair learning of and memory for unusual word associations, at least in young physicians.", "contents": "Attention, learning, and personality during ketamine emergence: a pilot study. To analyze the memory, learning, attention, and personality effects associated with emergence from partial ketamine induction, 11 resident physicians were tested. Ketamine was found to be truly dissociative in that it seemed to impair learning of and memory for unusual word associations, at least in young physicians."}
{"id": "PMID:1092210", "title": "Alupent syrup: results of a six-month trial in asthmatic children.", "content": "A six-month clinical trial of Alupent Syrup was conducted in 23 school-age children with bronchial asthma to assess the long-term efficacy and safety of this dosage form of metaproterenol sulfate. At three intervals during the study period, double-bind crossover tests against placebo were performed. Pulmonary function measurements revealed significant improvement in PEFR and FEV, after metaproterenol, and the results obtained at the end of ths study demonstrated that no tolerance to the drug had developed. The absence of adverse reactions attested to the safety of the drug in long-term management of childhood asthma.", "contents": "Alupent syrup: results of a six-month trial in asthmatic children. A six-month clinical trial of Alupent Syrup was conducted in 23 school-age children with bronchial asthma to assess the long-term efficacy and safety of this dosage form of metaproterenol sulfate. At three intervals during the study period, double-bind crossover tests against placebo were performed. Pulmonary function measurements revealed significant improvement in PEFR and FEV, after metaproterenol, and the results obtained at the end of ths study demonstrated that no tolerance to the drug had developed. The absence of adverse reactions attested to the safety of the drug in long-term management of childhood asthma."}
{"id": "PMID:1092207", "title": "The adult respiratory distress syndrome and pancuronium bromide.", "content": "The institution and maintenance of artificial ventilation for a patient with the adult respiratory distress syndrome (ARDS) is frequently difficult because the hypoxic patient is often confused, agitated, or combative. Pancuronium bromide, a recently introduced neuromuscular blocking agent, was used to facilitate artificial ventilation in 6 patients with ARDS. As compared with morphine sulfate and diazepam, pancuronium bromide has fewer cardiovascular and central-nervous system effects. It also has fewer cardiovascular effects than does curare, and its prolonged administration does not lead to a desensitization neuromuscular block as is seen with succinylcholine. However, continuous high-quality nursing care is necessary if this drug is used.", "contents": "The adult respiratory distress syndrome and pancuronium bromide. The institution and maintenance of artificial ventilation for a patient with the adult respiratory distress syndrome (ARDS) is frequently difficult because the hypoxic patient is often confused, agitated, or combative. Pancuronium bromide, a recently introduced neuromuscular blocking agent, was used to facilitate artificial ventilation in 6 patients with ARDS. As compared with morphine sulfate and diazepam, pancuronium bromide has fewer cardiovascular and central-nervous system effects. It also has fewer cardiovascular effects than does curare, and its prolonged administration does not lead to a desensitization neuromuscular block as is seen with succinylcholine. However, continuous high-quality nursing care is necessary if this drug is used."}
{"id": "PMID:1092214", "title": "Binding of anti-human immunoglobulins to human epidermal cells.", "content": "According to this study human skin scrapings bind anti-human IgE and IgM immunoglobulins as demonstrated by fluorescent miscroscopy; this is not dependent on the atopy of the subject.", "contents": "Binding of anti-human immunoglobulins to human epidermal cells. According to this study human skin scrapings bind anti-human IgE and IgM immunoglobulins as demonstrated by fluorescent miscroscopy; this is not dependent on the atopy of the subject."}
{"id": "PMID:1092222", "title": "Immune response to Escherichia coli.", "content": "Chickens from a line artifically selected for lew level of plasma corticosterone (LPC) produced higher antibody titers, produced antibody earlier, continued the production of antibody longer, responded to lower dosages of antigen, and had greater body weight gain than those from a line slected for a high level of plasma corticosterone (hpc). the HPC line resisted Escherichia coli challenge and antigen more effectively than the LPC line. It appears that, although the HPC line is more effective in its defense against bacterial infections, the LPC line can be aided more by vaccines.", "contents": "Immune response to Escherichia coli. Chickens from a line artifically selected for lew level of plasma corticosterone (LPC) produced higher antibody titers, produced antibody earlier, continued the production of antibody longer, responded to lower dosages of antigen, and had greater body weight gain than those from a line slected for a high level of plasma corticosterone (hpc). the HPC line resisted Escherichia coli challenge and antigen more effectively than the LPC line. It appears that, although the HPC line is more effective in its defense against bacterial infections, the LPC line can be aided more by vaccines."}
{"id": "PMID:1092224", "title": "Properties of free endotoxin from Pasteurella multocida.", "content": "Free endotoxin (FET) from virulent encapsulated Pasteurella multocida or from an avirulent nonencapsulated mutant is capable of inducing active immunity, but the lipopolysaccharide (LPS) moiety of the endotoxin is not. These results suggest that a protein of P multocida is involved in the stimulation of active immunity. The serologic specificity of the FET is associated with the LPS moiety, which is related to a heat extracted antigen that is used for serotyping P multocida. The FET is capable of producing widespread vascular alteration and death. It is present in the vascular system of turkeys with acute fowl cholera, and it can be detected with the \"Limulus\" test for endotoxins and with the gel diffusion precipitin test.", "contents": "Properties of free endotoxin from Pasteurella multocida. Free endotoxin (FET) from virulent encapsulated Pasteurella multocida or from an avirulent nonencapsulated mutant is capable of inducing active immunity, but the lipopolysaccharide (LPS) moiety of the endotoxin is not. These results suggest that a protein of P multocida is involved in the stimulation of active immunity. The serologic specificity of the FET is associated with the LPS moiety, which is related to a heat extracted antigen that is used for serotyping P multocida. The FET is capable of producing widespread vascular alteration and death. It is present in the vascular system of turkeys with acute fowl cholera, and it can be detected with the \"Limulus\" test for endotoxins and with the gel diffusion precipitin test."}
{"id": "PMID:1092226", "title": "Avian salmonellosis: detection and characterization of serologic response to major serogroup infections.", "content": "Serologic procedures for the diagnosis of salmonella infections in poultry flocks have evolved from laboratory and field macrotest procedures to recently reported microtests with savings of cost, time, and space. Microtest methods when used as the sensitive and specific microantiglobulin test provide a means of effectively detecting birds exposed to salmonella serogroup B, C, and D infections. Progress will be made in the better detection and control salmonella infections in poultry when attention is given to all major salmonella serogroups rather than a few selected serotypes.", "contents": "Avian salmonellosis: detection and characterization of serologic response to major serogroup infections. Serologic procedures for the diagnosis of salmonella infections in poultry flocks have evolved from laboratory and field macrotest procedures to recently reported microtests with savings of cost, time, and space. Microtest methods when used as the sensitive and specific microantiglobulin test provide a means of effectively detecting birds exposed to salmonella serogroup B, C, and D infections. Progress will be made in the better detection and control salmonella infections in poultry when attention is given to all major salmonella serogroups rather than a few selected serotypes."}
{"id": "PMID:1092227", "title": "Progress in the control of coccidiosis with anticoccidials and planned immunization.", "content": "New anticoccidials continue to be introduced to the poultry industry on an average of about one every 2 years. Revised requirements have increased costs of discovery, development, and approval by the Food and Drug Administration to several million dollars for each product. Gross sales of anticoccidials are now estimated to be in excess of $30,000,000 annually. Anticoccidials are almost universally used in starter rations for meat-type birds being raised under floor-pen management. Protection is more important with these fast-growing birds than with egg-producing types where immunity and cage layer management modify demands for anticoccidials. Coccidiosis mortality has generally been eliminated by all 25 approved anticoccidials if properly used. Selection of specific anticoccidials is based on the ability of the anticoccidial to: (1) improve weights and (2) feed conversion, and (3) to suppress development of lesions. Costs of the product may influence decisions on which one to use. With some anticoccidials the rapid emergence of drug resistant strains has been the biggest problem. \"Switching\" among the 13 classes of anticoccidials and the so-called \"shuttle program\" are common methods used to avoid development of resistance. Preventive medication will probably continue to constitute the major method of coccidiosis control with meat-type poultry if attempts to develop economic cage-type management do not supplant current litter and floor-pen management. Immunity acquired through incidental or planned immunization is more important in control of coccidiosis with layer and breeder flocks raised in floor-pens. Some progress in development of avirulent immunogenic strains of different species of coccidia has been reported and further research efforts in this direction should be encouraged.", "contents": "Progress in the control of coccidiosis with anticoccidials and planned immunization. New anticoccidials continue to be introduced to the poultry industry on an average of about one every 2 years. Revised requirements have increased costs of discovery, development, and approval by the Food and Drug Administration to several million dollars for each product. Gross sales of anticoccidials are now estimated to be in excess of $30,000,000 annually. Anticoccidials are almost universally used in starter rations for meat-type birds being raised under floor-pen management. Protection is more important with these fast-growing birds than with egg-producing types where immunity and cage layer management modify demands for anticoccidials. Coccidiosis mortality has generally been eliminated by all 25 approved anticoccidials if properly used. Selection of specific anticoccidials is based on the ability of the anticoccidial to: (1) improve weights and (2) feed conversion, and (3) to suppress development of lesions. Costs of the product may influence decisions on which one to use. With some anticoccidials the rapid emergence of drug resistant strains has been the biggest problem. \"Switching\" among the 13 classes of anticoccidials and the so-called \"shuttle program\" are common methods used to avoid development of resistance. Preventive medication will probably continue to constitute the major method of coccidiosis control with meat-type poultry if attempts to develop economic cage-type management do not supplant current litter and floor-pen management. Immunity acquired through incidental or planned immunization is more important in control of coccidiosis with layer and breeder flocks raised in floor-pens. Some progress in development of avirulent immunogenic strains of different species of coccidia has been reported and further research efforts in this direction should be encouraged."}
{"id": "PMID:1092229", "title": "Pathogenesis of Trypanosoma equiperdum in rabbits.", "content": "Seventeen rabbits experimentally infected with Trypanosoma equiperdum were examined for lesions at different times during a 5-month period. A chancroid developed at the inoculation site in the skin, and later, similar lesions appeared in skin of the ears, eyelids, and nose. The inflammatory reaction was primarily granulomatous, and viable trypanosomes were present in all skin lesions. The rabbits had reticuloendothelial hyperplasia of spleen and lymph nodes. In 3 rabbits, there was amyloid deposition in splenic lymph nodules and renal glomeruli. Amyloid had typical fibrillar appearance by electron microscopy (EM) and was reactive for immunoglobulin M (IgM) and immunoglobulin G (IgG) with fluorescent antibody (FA). With time, there was lessening capability of T equiperdum to agglutinate in antiserums of infected rabbits, suggesting that antigenic variation occurred during infection. The serum concentrations of IgM and IgG increased significantly in the infected rabbits and then remained elevated or they decreased, though fluctuating widely. Three of 5 infected rabbits demonstrated depressed antibody response to injected ovine erythrocytes.", "contents": "Pathogenesis of Trypanosoma equiperdum in rabbits. Seventeen rabbits experimentally infected with Trypanosoma equiperdum were examined for lesions at different times during a 5-month period. A chancroid developed at the inoculation site in the skin, and later, similar lesions appeared in skin of the ears, eyelids, and nose. The inflammatory reaction was primarily granulomatous, and viable trypanosomes were present in all skin lesions. The rabbits had reticuloendothelial hyperplasia of spleen and lymph nodes. In 3 rabbits, there was amyloid deposition in splenic lymph nodules and renal glomeruli. Amyloid had typical fibrillar appearance by electron microscopy (EM) and was reactive for immunoglobulin M (IgM) and immunoglobulin G (IgG) with fluorescent antibody (FA). With time, there was lessening capability of T equiperdum to agglutinate in antiserums of infected rabbits, suggesting that antigenic variation occurred during infection. The serum concentrations of IgM and IgG increased significantly in the infected rabbits and then remained elevated or they decreased, though fluctuating widely. Three of 5 infected rabbits demonstrated depressed antibody response to injected ovine erythrocytes."}
{"id": "PMID:1092230", "title": "Evaluation of an indirect method for estimating myofiber number in transverse sections of skeletal muscle.", "content": "The total number of myofibers contained in the transverse sections of 6 canine pectineus muscles was counted, using direct- and indirect-counting methods. For the direct-counting method, the transverse section of each muscle was photographed, from which a montage of each section was constructed. Then, the total number of myofibers contained in each montage was counted. The number obtained by the direct-counting method was used to determine the accuracy of the indirect-counting method. For the indirect-counting method, 40 randomly selected areas in each section were photographed. Then, the total number of myofibers contained in the randomly selected areas of each section was counted. From these counts, the total number of myofibers contained in each transverse section was calculated after the entire cross-sectional area of each section was determined by planimetry. The indirect-counting method was performed 3 times for each section. By direct count, the number of myofibers in a section was between 12,368 and 163,223. Generally, the indirect-counting method tended to overestimate the number of myofibers per section. The mean percentage error of estimates plus or minus standard error for all muscles and trials was 3.89 plus or minus 1.17; the range was minus 6.17 to +11.97. The percentage errors of the indirect counts were significantly correlated with the number of myofibers/sq mm and total number of myofibers/section; for every 1,000 myofibers/sq mm or 100,000 myofibers/section, an error of 2 or 5%, respectively, can be anticipated. The indirect-counting method utilized 10 to 20% of the man-hour effort required by the direct-counting method.", "contents": "Evaluation of an indirect method for estimating myofiber number in transverse sections of skeletal muscle. The total number of myofibers contained in the transverse sections of 6 canine pectineus muscles was counted, using direct- and indirect-counting methods. For the direct-counting method, the transverse section of each muscle was photographed, from which a montage of each section was constructed. Then, the total number of myofibers contained in each montage was counted. The number obtained by the direct-counting method was used to determine the accuracy of the indirect-counting method. For the indirect-counting method, 40 randomly selected areas in each section were photographed. Then, the total number of myofibers contained in the randomly selected areas of each section was counted. From these counts, the total number of myofibers contained in each transverse section was calculated after the entire cross-sectional area of each section was determined by planimetry. The indirect-counting method was performed 3 times for each section. By direct count, the number of myofibers in a section was between 12,368 and 163,223. Generally, the indirect-counting method tended to overestimate the number of myofibers per section. The mean percentage error of estimates plus or minus standard error for all muscles and trials was 3.89 plus or minus 1.17; the range was minus 6.17 to +11.97. The percentage errors of the indirect counts were significantly correlated with the number of myofibers/sq mm and total number of myofibers/section; for every 1,000 myofibers/sq mm or 100,000 myofibers/section, an error of 2 or 5%, respectively, can be anticipated. The indirect-counting method utilized 10 to 20% of the man-hour effort required by the direct-counting method."}
{"id": "PMID:1092231", "title": "Immunofluorescent studies on chronic pneumonia in swine with experimentally induced African swine fever.", "content": "Chronic pneumonia experimentally produced in 14 pigs with African swine fever (ASF) virus was studied by immunofluorescene (IF) and histopathologic techniques. Frozen sections prepared from pulmonary tissues of the infected pigs were stained with fluorescein-conjugated antiserums against ASF viral antigen, porcine immunoglobulin G (IgG), procine complement (C), and porcine fibrinogen. The viral antigen(s) was mainly seen in macrophages and cell debris in alveolar walls and lumens. This finding indicates that the virus replicated in the cytoplasm of alveolar macrophages that subsequently degenerated and released the viral antigen. Diffuse immunoglobulin (Ig) deposition was found in necrotic cells and debris. Immunoglobulin also was seen bound to intracytoplasmic inclusion bodies in some degenerating alveolar macrophages. This finding indicates that antibody against ASF viral antigen(s) excluded from blood circulation or produced by local immunocytes (or both) reacted with viral antigen at intramacrophage and extramacrophage levels and resulted in the formation of insoluble antigen-antibody (Ag-Ab) complexes. The participation of C in the immune complex was evident in the early stage of the pneumonia, but was less evident in the subsequent extensive, progressive necrotic processes. Fibrin deposits were visible only in the early necrotic area of alveolar walls and lumens. Possible mechanisms inducing extensive necrosis are discussed.", "contents": "Immunofluorescent studies on chronic pneumonia in swine with experimentally induced African swine fever. Chronic pneumonia experimentally produced in 14 pigs with African swine fever (ASF) virus was studied by immunofluorescene (IF) and histopathologic techniques. Frozen sections prepared from pulmonary tissues of the infected pigs were stained with fluorescein-conjugated antiserums against ASF viral antigen, porcine immunoglobulin G (IgG), procine complement (C), and porcine fibrinogen. The viral antigen(s) was mainly seen in macrophages and cell debris in alveolar walls and lumens. This finding indicates that the virus replicated in the cytoplasm of alveolar macrophages that subsequently degenerated and released the viral antigen. Diffuse immunoglobulin (Ig) deposition was found in necrotic cells and debris. Immunoglobulin also was seen bound to intracytoplasmic inclusion bodies in some degenerating alveolar macrophages. This finding indicates that antibody against ASF viral antigen(s) excluded from blood circulation or produced by local immunocytes (or both) reacted with viral antigen at intramacrophage and extramacrophage levels and resulted in the formation of insoluble antigen-antibody (Ag-Ab) complexes. The participation of C in the immune complex was evident in the early stage of the pneumonia, but was less evident in the subsequent extensive, progressive necrotic processes. Fibrin deposits were visible only in the early necrotic area of alveolar walls and lumens. Possible mechanisms inducing extensive necrosis are discussed."}
{"id": "PMID:1092232", "title": "Development of specific in vitro lymphocyte responses in cattle infected with Mycobacterium bovis and with Mycobacterium avium.", "content": "Two calves infected with Mycobacterium avium and another 2 with Mycobacterium bovis were studied for development and specificity of lymphocyte activation responses and skin reactivity to purified protein derivatives (PPD). Lymphocytes cultured in vitro from infected calves manifested significant uptake of tritiated thymidine in response to the PPD prepared from the homologous organisms, whereas heterologous PPD produced little or no stimulation. The time of appearance of specifically sensitized lymphocytes was studied, as was the specificity of skin reactivity.", "contents": "Development of specific in vitro lymphocyte responses in cattle infected with Mycobacterium bovis and with Mycobacterium avium. Two calves infected with Mycobacterium avium and another 2 with Mycobacterium bovis were studied for development and specificity of lymphocyte activation responses and skin reactivity to purified protein derivatives (PPD). Lymphocytes cultured in vitro from infected calves manifested significant uptake of tritiated thymidine in response to the PPD prepared from the homologous organisms, whereas heterologous PPD produced little or no stimulation. The time of appearance of specifically sensitized lymphocytes was studied, as was the specificity of skin reactivity."}
{"id": "PMID:1092234", "title": "Interacinar pathways in the human lung.", "content": "Normal lung specimens from patients 18 to 86 years of age were inflated, fixed, and cleared. After micropuncture of the distal airspaces and injection of silicone rubber, the dissemination pattern was studied by cinematography. Free interacinar flow was commonly observed. The major pathways of spread among adjacent acini were the interacinar ducts. These were short, tubular structures 200 mum in diameter that were continuous with respiratory bronchioles and alveolar ducts. The flow of silicone rubber was impeded only by the septa of the secondary lobule of Miller. Our finding support the view that the smallest morphologic unit of airspace disease is more likely to be the secondary lobule than the acinus.", "contents": "Interacinar pathways in the human lung. Normal lung specimens from patients 18 to 86 years of age were inflated, fixed, and cleared. After micropuncture of the distal airspaces and injection of silicone rubber, the dissemination pattern was studied by cinematography. Free interacinar flow was commonly observed. The major pathways of spread among adjacent acini were the interacinar ducts. These were short, tubular structures 200 mum in diameter that were continuous with respiratory bronchioles and alveolar ducts. The flow of silicone rubber was impeded only by the septa of the secondary lobule of Miller. Our finding support the view that the smallest morphologic unit of airspace disease is more likely to be the secondary lobule than the acinus."}
{"id": "PMID:1092237", "title": "Cavitary pulmonary cryptococcosis complicated by aspergilloma.", "content": "A roentgenogram of a 31-year-old office worker who had developed pleuritic pain showed multiple abscess cavities in the left lower lobe, and a clinical diagnosis of cryptococcosis was considered because of cryptococci recovered from a sputum smear and a positive indirect fluorescent antibody test. Lobectomy (after unsuccessful amphotericin therapy) proved the presence of a destructive cryptococcosis with Aspergillus proliferating in the cavities.", "contents": "Cavitary pulmonary cryptococcosis complicated by aspergilloma. A roentgenogram of a 31-year-old office worker who had developed pleuritic pain showed multiple abscess cavities in the left lower lobe, and a clinical diagnosis of cryptococcosis was considered because of cryptococci recovered from a sputum smear and a positive indirect fluorescent antibody test. Lobectomy (after unsuccessful amphotericin therapy) proved the presence of a destructive cryptococcosis with Aspergillus proliferating in the cavities."}
{"id": "PMID:1092239", "title": "Effect of continuous positive pressure ventilation on cardiac output.", "content": "Ten mongrel dogs were placed on 5 and 10 cm. H20 of CPPV. Cardiac output, pulmonary vascular resistance and pleural pressures were measured. At 5 cm. H20/CPPV there was no significant change in cardiac output or pulmonary vascular resistance. At 10 cm. H20 CPPV cardiac output significantly decreased and pulmonary vascular resistance increased. The physiologic basis for these changes is discussed and related to the clinical situation.", "contents": "Effect of continuous positive pressure ventilation on cardiac output. Ten mongrel dogs were placed on 5 and 10 cm. H20 of CPPV. Cardiac output, pulmonary vascular resistance and pleural pressures were measured. At 5 cm. H20/CPPV there was no significant change in cardiac output or pulmonary vascular resistance. At 10 cm. H20 CPPV cardiac output significantly decreased and pulmonary vascular resistance increased. The physiologic basis for these changes is discussed and related to the clinical situation."}
{"id": "PMID:1092240", "title": "Changing surgical indications in azotemic secondary hyperparathyroidism.", "content": "It would appear that the initial enthusiasm for surgery in azotemic secondary hyperparathyroidism was unsupported by conclusive data, but that a therapeutic alternative recommending surgical abstinence represents an equally extreme position, particularly since the long-term effects of sustained mild hyperparathyroidism are unknown. The proper course at present for the patient's best interests might best be described as selective. Final resolution of conditional therapy in azotemic hyperparathyroidism awaits the completion of objective studies.", "contents": "Changing surgical indications in azotemic secondary hyperparathyroidism. It would appear that the initial enthusiasm for surgery in azotemic secondary hyperparathyroidism was unsupported by conclusive data, but that a therapeutic alternative recommending surgical abstinence represents an equally extreme position, particularly since the long-term effects of sustained mild hyperparathyroidism are unknown. The proper course at present for the patient's best interests might best be described as selective. Final resolution of conditional therapy in azotemic hyperparathyroidism awaits the completion of objective studies."}
{"id": "PMID:1092243", "title": "Penetrating keratoplasty in a patient with 75% third degree burns.", "content": "A 25-year-old woman was severely burned in a gasoline explosion in her home. One month after her admission to the hospital a mycotic corneal ulcer, caused by Candida albicans, developed and progressed to a descemetocele in spite of medical therapy. A therapeutic penetrating keratoplasty was performed and the graft remained clear until the present time (10 months of follow-up). The one episode of clinical corneal graft rejection occurred at the same time the first evidence of return of immune competence was seen in her skin grafts. The corneal rejection episode was successfully treated by topical corticosteroids.", "contents": "Penetrating keratoplasty in a patient with 75% third degree burns. A 25-year-old woman was severely burned in a gasoline explosion in her home. One month after her admission to the hospital a mycotic corneal ulcer, caused by Candida albicans, developed and progressed to a descemetocele in spite of medical therapy. A therapeutic penetrating keratoplasty was performed and the graft remained clear until the present time (10 months of follow-up). The one episode of clinical corneal graft rejection occurred at the same time the first evidence of return of immune competence was seen in her skin grafts. The corneal rejection episode was successfully treated by topical corticosteroids."}
{"id": "PMID:1092244", "title": "A simple levator resection operation.", "content": "A simple procedure for identification and isolation of the levator muscle is presented. With this technique the conjunctiva need not be cut from its tarsal connection. No extra sutures are used to create a lid fold. It is emphasized again that the criterion for the amount of levator resection is not the degree of ptosis but the amount of levator action.", "contents": "A simple levator resection operation. A simple procedure for identification and isolation of the levator muscle is presented. With this technique the conjunctiva need not be cut from its tarsal connection. No extra sutures are used to create a lid fold. It is emphasized again that the criterion for the amount of levator resection is not the degree of ptosis but the amount of levator action."}
{"id": "PMID:1092241", "title": "[Right ventricle with double outlet associated to total anomalous pulmonary venous drainage to innominate vein].", "content": "The clinical findings of 2 patients are studied with an association of malformations not previously reported: double outlet right ventricle and total anomalous pulmonary venous connection to the innominate vein. The following features are worth while to be underlined: 1. Both patients showed a discordance between viscero-atrial situs and direction of the apex; 2. In both cases, particularly in case 2, there existed a very unusual spacial relationship between ventricles, the right ventricle being superior with respect to the left ventricular cavity; 3. From the embryological viewpoint, this ventricular arrangement is supposed to be due to an arrest of the development of the heart in the straight tube stage, there being, however, an upwards expansion of the atrioventricular canal and common atrium; 4. An accurate diagnosis of the common or divided nature of the atrioventricular valves has been impossible in these cases; 5. These combination of lesions constitutes a challenge to cardiologists and surgeons as far as the surgical indication is concerned. In that sense, the simple anastomosis of the common pulmonary vein to the left atrium, although is not a radical cure, may be a feasible and beneficial procedure.", "contents": "[Right ventricle with double outlet associated to total anomalous pulmonary venous drainage to innominate vein]. The clinical findings of 2 patients are studied with an association of malformations not previously reported: double outlet right ventricle and total anomalous pulmonary venous connection to the innominate vein. The following features are worth while to be underlined: 1. Both patients showed a discordance between viscero-atrial situs and direction of the apex; 2. In both cases, particularly in case 2, there existed a very unusual spacial relationship between ventricles, the right ventricle being superior with respect to the left ventricular cavity; 3. From the embryological viewpoint, this ventricular arrangement is supposed to be due to an arrest of the development of the heart in the straight tube stage, there being, however, an upwards expansion of the atrioventricular canal and common atrium; 4. An accurate diagnosis of the common or divided nature of the atrioventricular valves has been impossible in these cases; 5. These combination of lesions constitutes a challenge to cardiologists and surgeons as far as the surgical indication is concerned. In that sense, the simple anastomosis of the common pulmonary vein to the left atrium, although is not a radical cure, may be a feasible and beneficial procedure."}
{"id": "PMID:1092245", "title": "Benign paroxysmal positonal vertigo: A clinical study.", "content": "Summary--Ru-Vert, a combination product, containing in each tablet, 25 mg of pentylenetetrazol, 12.5 mg of pheniramine maleate, and 50 mg of nictonic acid, was evaluated in the treatment of seventeen patients with benign paroxysmal positional vertigo. The study was double-blind with crossover. Ru-Vert at a dosage of two tablets t.i.d. was found to reduce significantly the nystagmus and the vertigo induced by Hallpike maneuvers in these patients.", "contents": "Benign paroxysmal positonal vertigo: A clinical study. Summary--Ru-Vert, a combination product, containing in each tablet, 25 mg of pentylenetetrazol, 12.5 mg of pheniramine maleate, and 50 mg of nictonic acid, was evaluated in the treatment of seventeen patients with benign paroxysmal positional vertigo. The study was double-blind with crossover. Ru-Vert at a dosage of two tablets t.i.d. was found to reduce significantly the nystagmus and the vertigo induced by Hallpike maneuvers in these patients."}
{"id": "PMID:1092246", "title": "Serum and urine polyamines in cancer.", "content": "Polyamines in serum and urine have been found to be elevated in patients with cancer. A variety of methods employed for these measurements are discussed. Normal values obtained by the most recent methods are presented, and a survey has been made of polyamine levels in cancer patients.", "contents": "Serum and urine polyamines in cancer. Polyamines in serum and urine have been found to be elevated in patients with cancer. A variety of methods employed for these measurements are discussed. Normal values obtained by the most recent methods are presented, and a survey has been made of polyamine levels in cancer patients."}
{"id": "PMID:1092247", "title": "McArdle's disease: a review.", "content": "The clinical features of McArdle's disease (inherited deficiency of skeletal muscle phosphorylase) and the histological and biochemical changes are described. Their possible causes are discussed in the light of recent knowledge of the biochemistry of muscular contraction. Diagnostic tests are detailed. Attention is drawn to the possibility that the disease may be due to primary defect of motoneurons.", "contents": "McArdle's disease: a review. The clinical features of McArdle's disease (inherited deficiency of skeletal muscle phosphorylase) and the histological and biochemical changes are described. Their possible causes are discussed in the light of recent knowledge of the biochemistry of muscular contraction. Diagnostic tests are detailed. Attention is drawn to the possibility that the disease may be due to primary defect of motoneurons."}
{"id": "PMID:1092248", "title": "Current status of zinc deficiency in the pathogenesis of neurological, dermatological and musculoskeletal disorders.", "content": "Recent clinical and experimental studies suggest that zinc deficiency may play an important role in the pathogenesis of (1) acrodermatitis enteropathica, and in certain cases of (2) hypogonadal dwarfism, (3) congenital malformations, (4) hypogeusia and hyposmia, (5) nyctalopia and (6) impaired wound healing. Distrubances of zinc metabolism also occur in a broad spectrum of other clinical disorders. The pathophysiological factors which are responsible for hypozincemia include: (1) nutritional deficiency and/or intestinal malabsorption of zinc; (2) hyperzincuria secondary to aminoaciduria; (3) hormonal effects (cortisol, growth hormone, estrogens); (4) hypoalbuminemia; and (5) effects of leukocytic endogenous mediator. The clinical diagnosis of zinc deficiency in patients with specific neurological, dermatological and musculoskeletal disorders is complicated by the complex interactions of these pathophysiological factors and by the need for more dependable laboratory indices of zinc deprivation.", "contents": "Current status of zinc deficiency in the pathogenesis of neurological, dermatological and musculoskeletal disorders. Recent clinical and experimental studies suggest that zinc deficiency may play an important role in the pathogenesis of (1) acrodermatitis enteropathica, and in certain cases of (2) hypogonadal dwarfism, (3) congenital malformations, (4) hypogeusia and hyposmia, (5) nyctalopia and (6) impaired wound healing. Distrubances of zinc metabolism also occur in a broad spectrum of other clinical disorders. The pathophysiological factors which are responsible for hypozincemia include: (1) nutritional deficiency and/or intestinal malabsorption of zinc; (2) hyperzincuria secondary to aminoaciduria; (3) hormonal effects (cortisol, growth hormone, estrogens); (4) hypoalbuminemia; and (5) effects of leukocytic endogenous mediator. The clinical diagnosis of zinc deficiency in patients with specific neurological, dermatological and musculoskeletal disorders is complicated by the complex interactions of these pathophysiological factors and by the need for more dependable laboratory indices of zinc deprivation."}
{"id": "PMID:1092249", "title": "The measurement of erythropoietin.", "content": "The bioassay of erythropoietin in animals is reviewed. The techniques employed are those of hypertransfusion and hypoxia using polycythemic mice.", "contents": "The measurement of erythropoietin. The bioassay of erythropoietin in animals is reviewed. The techniques employed are those of hypertransfusion and hypoxia using polycythemic mice."}
{"id": "PMID:1092257", "title": "[Effect of antibiotics on the indices on nonspecific immunity].", "content": "Multiple (for 14 and 20 days) administrations of antibiotics, such as tetraolean, tetracycline and penicillin from the beginning of the animal immunization (rabbits and albino rats) with typhoid fever and candidosis vaccines inhibited activity of both the non-specific and the specific immunity.", "contents": "[Effect of antibiotics on the indices on nonspecific immunity]. Multiple (for 14 and 20 days) administrations of antibiotics, such as tetraolean, tetracycline and penicillin from the beginning of the animal immunization (rabbits and albino rats) with typhoid fever and candidosis vaccines inhibited activity of both the non-specific and the specific immunity."}
{"id": "PMID:1092258", "title": "[Caffeine as an inhibitor of the conjugation transfer of R-factors. A study of the quantitative effect of caffeine on the conjugation transfer of R-factors].", "content": "The effect of caffeine on the conjugation transfer of R-factors was studied using a standartized kinetic conjugation system described earlier. It was shown that caffeine had a pronounced inhibitory effect on R-factor transfer. The inhibition coefficients at a caffeine concentration of 2000 gamma/ml were from 2.5 (when using strain CSH-2 R222 of E. coli as the donor) to 13.8 (when using strain J5-3 RI drd of E. coli as the donor). Higher concentrations of caffeine induced a significant increase in the coefficient. Since R-transfer inhibition was induced by rather high concentrations of caffeine, the effect of equivalent concentrations of some normal metabolites of the purine and pyrimidine series, such as ATP, guanine, cytosine and thymine was studied. It was shown that the substances had no inhibitory effect on R-factor transfer. Inhibition of P-transfer by caffeine did not depend on either the type of R-factor or the microbial host.", "contents": "[Caffeine as an inhibitor of the conjugation transfer of R-factors. A study of the quantitative effect of caffeine on the conjugation transfer of R-factors]. The effect of caffeine on the conjugation transfer of R-factors was studied using a standartized kinetic conjugation system described earlier. It was shown that caffeine had a pronounced inhibitory effect on R-factor transfer. The inhibition coefficients at a caffeine concentration of 2000 gamma/ml were from 2.5 (when using strain CSH-2 R222 of E. coli as the donor) to 13.8 (when using strain J5-3 RI drd of E. coli as the donor). Higher concentrations of caffeine induced a significant increase in the coefficient. Since R-transfer inhibition was induced by rather high concentrations of caffeine, the effect of equivalent concentrations of some normal metabolites of the purine and pyrimidine series, such as ATP, guanine, cytosine and thymine was studied. It was shown that the substances had no inhibitory effect on R-factor transfer. Inhibition of P-transfer by caffeine did not depend on either the type of R-factor or the microbial host."}
{"id": "PMID:1092259", "title": "[Study of the conditions for mass transfer in the nystatin biosynthetic process].", "content": "The kinetics of the biomass growth, nystatin production and carbohydrate consumption in cultures of act. noursei in 180 liter fermenters was studied. Under conditions of intensive agitation (Klas greater than 6) dependence of the specific productivity on the specific rates of growth and carbohydrate consumption was found. No such dependence between the above parameters were observed under conditions of poor agitation (Klas smaller than 3.5). It was shown that at the beginning of the cultivation process the coefficient of oxygen transfer Kla rapidly decreased. Later the value of Kla decreased slightly. Dependence of the maximum activity of the culture fluid on the average coefficient of masstransfer with respect to oxygen was observed.", "contents": "[Study of the conditions for mass transfer in the nystatin biosynthetic process]. The kinetics of the biomass growth, nystatin production and carbohydrate consumption in cultures of act. noursei in 180 liter fermenters was studied. Under conditions of intensive agitation (Klas greater than 6) dependence of the specific productivity on the specific rates of growth and carbohydrate consumption was found. No such dependence between the above parameters were observed under conditions of poor agitation (Klas smaller than 3.5). It was shown that at the beginning of the cultivation process the coefficient of oxygen transfer Kla rapidly decreased. Later the value of Kla decreased slightly. Dependence of the maximum activity of the culture fluid on the average coefficient of masstransfer with respect to oxygen was observed."}
{"id": "PMID:1092260", "title": "[Study of the effect of nystatin and mycoheptin on the intrauterine development of the rat fetus].", "content": "The effect of nystatin and mycoheptyne on pregnancy and fetus of rats of Wister line was studied after single and multiple oral administrations of the drugs. Nystatin was used in single doses of 3000, 500 and 100 mg/kg. In chronic experiments the dose was 500 mg/kg. It was found that nystatin had a slight abortive effect when used during the whole period of pregnancy. Mycoheptyne was used in single doses of 1000, 100 and 20 mg/kg. In chronic experiments the dose was 100 mg/kg. It was noted that mycoheptyne had a higher toxic effect on the pregnant rats than nystatin. The animal death and abortus were observed more often. The level of the changes depended on the dose, time and period of the antibiotic use. No defects in the rat fetus were found.", "contents": "[Study of the effect of nystatin and mycoheptin on the intrauterine development of the rat fetus]. The effect of nystatin and mycoheptyne on pregnancy and fetus of rats of Wister line was studied after single and multiple oral administrations of the drugs. Nystatin was used in single doses of 3000, 500 and 100 mg/kg. In chronic experiments the dose was 500 mg/kg. It was found that nystatin had a slight abortive effect when used during the whole period of pregnancy. Mycoheptyne was used in single doses of 1000, 100 and 20 mg/kg. In chronic experiments the dose was 100 mg/kg. It was noted that mycoheptyne had a higher toxic effect on the pregnant rats than nystatin. The animal death and abortus were observed more often. The level of the changes depended on the dose, time and period of the antibiotic use. No defects in the rat fetus were found."}
{"id": "PMID:1092261", "title": "[\"Typical\" and \"atypical\" damages of the organ of hearing from the administration of streptomycin].", "content": "Two areas of the damage localization in the organs of hearing after streptomycin use were found in the experiments with guinea pigs, i.e. (1) the Corti's organ of the lower part of the basal helix, the zone of perception of the maximum frequency sounds and (2) the tympanic muscles. Administration of streptomycin subcutaneously in a daily dose of 300 mg/kg for 50 days resulted in pronounced atrophic changes in the fibres of m. tensor tympani and some atony of m. stapedius. On the basis of the histological examination of the tympanic muscles after prolonged use of streptomycin and comparison of the periods of the tonus reduction in the skeletal muscles and Preier reflex after a single administration of streptomycin in maximum tolerating doses, it was concluded that streptomycin affected the state of the tympanic muscles as a myorelaxant. The atrophic changes in the tensor as a result of prolonged streptomycin use was due to chronic atony of the muscle. The decrease in the contraction capacity of the tensor must result in loosening of the drum membrane tension, impairement of the muscle activity coordination, decreased ability for elimation of the auditory ossicle fluctuation. Increased audibility limits within middle and low frequencies and noise in the ears during prolonged treatment of patients with streptomycin may be associated with chronic atony of the tympanic muscles and mainly tensor.", "contents": "[\"Typical\" and \"atypical\" damages of the organ of hearing from the administration of streptomycin]. Two areas of the damage localization in the organs of hearing after streptomycin use were found in the experiments with guinea pigs, i.e. (1) the Corti's organ of the lower part of the basal helix, the zone of perception of the maximum frequency sounds and (2) the tympanic muscles. Administration of streptomycin subcutaneously in a daily dose of 300 mg/kg for 50 days resulted in pronounced atrophic changes in the fibres of m. tensor tympani and some atony of m. stapedius. On the basis of the histological examination of the tympanic muscles after prolonged use of streptomycin and comparison of the periods of the tonus reduction in the skeletal muscles and Preier reflex after a single administration of streptomycin in maximum tolerating doses, it was concluded that streptomycin affected the state of the tympanic muscles as a myorelaxant. The atrophic changes in the tensor as a result of prolonged streptomycin use was due to chronic atony of the muscle. The decrease in the contraction capacity of the tensor must result in loosening of the drum membrane tension, impairement of the muscle activity coordination, decreased ability for elimation of the auditory ossicle fluctuation. Increased audibility limits within middle and low frequencies and noise in the ears during prolonged treatment of patients with streptomycin may be associated with chronic atony of the tympanic muscles and mainly tensor."}
{"id": "PMID:1092262", "title": "Toxigenic thermophilic and thermotolerant fungi.", "content": "Twenty-three isolates of fungi, representing 13 thermophilic and thermotolerant species, were bioassayed for toxigenicity to brine shrimp, chicken embryos, and rats. Thirteen isolates representing nine genera were highly toxic to at least two of the three systems. Seven additional isolates of five genera were slightly toxic.", "contents": "Toxigenic thermophilic and thermotolerant fungi. Twenty-three isolates of fungi, representing 13 thermophilic and thermotolerant species, were bioassayed for toxigenicity to brine shrimp, chicken embryos, and rats. Thirteen isolates representing nine genera were highly toxic to at least two of the three systems. Seven additional isolates of five genera were slightly toxic."}
{"id": "PMID:1092263", "title": "Rapid determination of the amount of cetylpyridinium chloride bound by bacteria.", "content": "A modification of the Colowick and Womack procedure for measuring ligand binding by macromolecules is described for drug binding by bacteria. This technique is based on the determination of drug concentration in the dialysate from a bacteria-drug mixture at equilibrium. The dialysis cell of the original procedure was replaced by a Minibeaker (Bio-Rad), which has a larger membrane surface area, and the dialysate was continuously monitored with a spectrophotometer equipped with a flow cell. With this system, only 3 min was required to determine the amount of cetylpyridinium chloride bound by Escherichia coli K-12 strain P678. Possible applications of the technique are discussed.", "contents": "Rapid determination of the amount of cetylpyridinium chloride bound by bacteria. A modification of the Colowick and Womack procedure for measuring ligand binding by macromolecules is described for drug binding by bacteria. This technique is based on the determination of drug concentration in the dialysate from a bacteria-drug mixture at equilibrium. The dialysis cell of the original procedure was replaced by a Minibeaker (Bio-Rad), which has a larger membrane surface area, and the dialysate was continuously monitored with a spectrophotometer equipped with a flow cell. With this system, only 3 min was required to determine the amount of cetylpyridinium chloride bound by Escherichia coli K-12 strain P678. Possible applications of the technique are discussed."}
{"id": "PMID:1092264", "title": "Improved membrane filter method for fecal coliform analysis.", "content": "A two-layer agar method has been developed which consistently yields higher recovery of fecal coliforms on membrane filters when compared to the existing membrane fecal coliform procedure. This method has been evaluated by three laboratories using samples of raw and chlorinated waste water, and reservoir, river, and marine waters. Verification of 1,013 fecal coliform colonies isolated from 61 water samples averaged 92% on this proposed procedure. Comparison with the Standard Methods membrane fecal coliform procedure revealed the two-layer agar method had an overall increased sensitivity to fecal coliform detection in these waters. It is therefore proposed that this procedure be evaluated as an alternative to the Standard Methods fecal coliform membrane Filter test in the examination of chlorinated secondary effluents, marine waters, and any natural waters that may contain pollutants with heavy metal ions.", "contents": "Improved membrane filter method for fecal coliform analysis. A two-layer agar method has been developed which consistently yields higher recovery of fecal coliforms on membrane filters when compared to the existing membrane fecal coliform procedure. This method has been evaluated by three laboratories using samples of raw and chlorinated waste water, and reservoir, river, and marine waters. Verification of 1,013 fecal coliform colonies isolated from 61 water samples averaged 92% on this proposed procedure. Comparison with the Standard Methods membrane fecal coliform procedure revealed the two-layer agar method had an overall increased sensitivity to fecal coliform detection in these waters. It is therefore proposed that this procedure be evaluated as an alternative to the Standard Methods fecal coliform membrane Filter test in the examination of chlorinated secondary effluents, marine waters, and any natural waters that may contain pollutants with heavy metal ions."}
{"id": "PMID:1092265", "title": "Violet red bile 2 agar for stressed coliforms.", "content": "Counts on a new, autoclave-sterilizable violet red bile (VRB-2) agar were compared with counts on freshly boiled VRB agar. Yields on VRB-2 agar averaged 217, 180, 130, and 112% of counts obtained on the control medium for samples of water, cottage cheese, frozen vegetables, and raw milk, respectively. The general principle used for the development of VRB-2 agar could be applied to many other kinds of selective plating media.", "contents": "Violet red bile 2 agar for stressed coliforms. Counts on a new, autoclave-sterilizable violet red bile (VRB-2) agar were compared with counts on freshly boiled VRB agar. Yields on VRB-2 agar averaged 217, 180, 130, and 112% of counts obtained on the control medium for samples of water, cottage cheese, frozen vegetables, and raw milk, respectively. The general principle used for the development of VRB-2 agar could be applied to many other kinds of selective plating media."}
{"id": "PMID:1092266", "title": "Repair and enumeration of injured coliforms by a plating procedure.", "content": "Surface plating of coliforms on Trypticase soy agar, followed by 1 to 2 h of incubation at 25 C and subsequent overlay with violet red bile agar, was found to be a useful method for the repair and enumeration of coliforms injured by freezing.", "contents": "Repair and enumeration of injured coliforms by a plating procedure. Surface plating of coliforms on Trypticase soy agar, followed by 1 to 2 h of incubation at 25 C and subsequent overlay with violet red bile agar, was found to be a useful method for the repair and enumeration of coliforms injured by freezing."}
{"id": "PMID:1092272", "title": "Jejunal microflora in malnourished Gambian children.", "content": "Growth of bacteria greater than 10-5 organisms/ml was found in 22 children, of whom 17 gave a histroy of chronic diarrhoea. The other 8 children had either no diarrhoea or where having an acute attack lasting for a few days. In those with chronic diarrhoea, Esch. coli, bacteroides, and enterococci tended to occur more frequently, whereas streptococci occurred more frequently in those with acute diarrhoea. Bacilli, staphylococci, micrococci, klebsiellas, pseudomonads, and candidas often occurred in both groups and in large numbers in those with chronic diarrhoea. This confirms previous reports in other parts of the world that some children with malnutrition have considerable bacterial contamination of the jejunum, and that this may be of aetiological significance as a cause of much of the diarrhoea seen in malnourished children. It is possible too that this may be important in the pathogenesis of malnutrition. The presence of intestinal parasites in these malnourished children is also noted. A double-blind trial in the use of antibiotics in this condition is advocated to determine whether it is possible to break the diarrhoea-malabsorption-malnutrition cycle. At the same time the effect of simply removing the child to a more sanitary environment, together with an estimate of the natural clearance of bacteria from the upper intestine, should be evaluated.", "contents": "Jejunal microflora in malnourished Gambian children. Growth of bacteria greater than 10-5 organisms/ml was found in 22 children, of whom 17 gave a histroy of chronic diarrhoea. The other 8 children had either no diarrhoea or where having an acute attack lasting for a few days. In those with chronic diarrhoea, Esch. coli, bacteroides, and enterococci tended to occur more frequently, whereas streptococci occurred more frequently in those with acute diarrhoea. Bacilli, staphylococci, micrococci, klebsiellas, pseudomonads, and candidas often occurred in both groups and in large numbers in those with chronic diarrhoea. This confirms previous reports in other parts of the world that some children with malnutrition have considerable bacterial contamination of the jejunum, and that this may be of aetiological significance as a cause of much of the diarrhoea seen in malnourished children. It is possible too that this may be important in the pathogenesis of malnutrition. The presence of intestinal parasites in these malnourished children is also noted. A double-blind trial in the use of antibiotics in this condition is advocated to determine whether it is possible to break the diarrhoea-malabsorption-malnutrition cycle. At the same time the effect of simply removing the child to a more sanitary environment, together with an estimate of the natural clearance of bacteria from the upper intestine, should be evaluated."}
{"id": "PMID:1092273", "title": "Phagocytosis and killing ability of Candida albicans by blood leucocytes of healthy term and preterm babies.", "content": "The phagocytosis and killing ability of leucocytes of 24 term and 22 preterm babies against Candida albicans were studies during the first 20 days of life because of the increased incidence of monilia infection at this time. The leucocytes of 14 adults aged 20 to 30 years served as controls. The phagocytosis ability of the leucocytes in adults, term, and preterm babies was not significantly different, mean values being respectively 66-7%, 57%, and 56-9%. The killing ability of the leycocytes in term and preterm babies was lower when compared with that of adult leucocytes (P less than 0-001 for term and P less than 0-01 for preterm infants). The mean value in adults was 27-5%, in term infants 9-7%, and in preterm infants 9-5%. It is suggested that as the addition of adult serum did not improve the candidacidal ability of newborn leucocytes, the killing defect should be sought in the leucocyte itself and not in serum factors.", "contents": "Phagocytosis and killing ability of Candida albicans by blood leucocytes of healthy term and preterm babies. The phagocytosis and killing ability of leucocytes of 24 term and 22 preterm babies against Candida albicans were studies during the first 20 days of life because of the increased incidence of monilia infection at this time. The leucocytes of 14 adults aged 20 to 30 years served as controls. The phagocytosis ability of the leucocytes in adults, term, and preterm babies was not significantly different, mean values being respectively 66-7%, 57%, and 56-9%. The killing ability of the leycocytes in term and preterm babies was lower when compared with that of adult leucocytes (P less than 0-001 for term and P less than 0-01 for preterm infants). The mean value in adults was 27-5%, in term infants 9-7%, and in preterm infants 9-5%. It is suggested that as the addition of adult serum did not improve the candidacidal ability of newborn leucocytes, the killing defect should be sought in the leucocyte itself and not in serum factors."}
{"id": "PMID:1092274", "title": "Macrophage-lymphocyte clustering in rheumatoid arthritis.", "content": "The cells in synovial fluid from patients with rheumatoid arthritis contain a small percentage of macrophages. Such macrophages were isolated and cultured alone and with homologous and heterologous lymphocytes for 24 hours, in an attempt to identify possible contact between living lymphocytes and macrophages. Such contact was found, with clustering of lymphocytes around macrophages, and was particularly well shown by scanning electron microscopy.", "contents": "Macrophage-lymphocyte clustering in rheumatoid arthritis. The cells in synovial fluid from patients with rheumatoid arthritis contain a small percentage of macrophages. Such macrophages were isolated and cultured alone and with homologous and heterologous lymphocytes for 24 hours, in an attempt to identify possible contact between living lymphocytes and macrophages. Such contact was found, with clustering of lymphocytes around macrophages, and was particularly well shown by scanning electron microscopy."}
{"id": "PMID:1092276", "title": "Suppression of malaria with monthly administration of combined sulphadoxine and pyrimethamine.", "content": "A trial of suppression of malaria by administration of combined sulphadoxine-pyrimethamine tablets every 28 days was undertaken in West Malaysia during 1972. One thousand subjects were followed over a 10-month period, including control groups on placebo and on weekly chloroquine. Subjects were examined monthly for parasitaemia, drug reactions, leucopenia, teratogenicity and haemolysis among the subjects deficient in glucose-6-phosphate dehydrogenase. Rates of new infections in the placebo group were 8.0% with Plasmodium falciparum and 6.2% with P. vivax; in the group receiving weekly chloroquine, 5.1% P. falciparum and 0.3% P. vivax; and in the group receiving monthly sulphadoxine-pyrimethamine, 0.3% P. Falciparum and 1.0% P. vivax. The effective rate of cure of new infections with P. falciparum by a single suppressive dose of combined sulphadoxine-pyrimethamine given the following month was 88.7%. No serious side effects were observed.", "contents": "Suppression of malaria with monthly administration of combined sulphadoxine and pyrimethamine. A trial of suppression of malaria by administration of combined sulphadoxine-pyrimethamine tablets every 28 days was undertaken in West Malaysia during 1972. One thousand subjects were followed over a 10-month period, including control groups on placebo and on weekly chloroquine. Subjects were examined monthly for parasitaemia, drug reactions, leucopenia, teratogenicity and haemolysis among the subjects deficient in glucose-6-phosphate dehydrogenase. Rates of new infections in the placebo group were 8.0% with Plasmodium falciparum and 6.2% with P. vivax; in the group receiving weekly chloroquine, 5.1% P. falciparum and 0.3% P. vivax; and in the group receiving monthly sulphadoxine-pyrimethamine, 0.3% P. Falciparum and 1.0% P. vivax. The effective rate of cure of new infections with P. falciparum by a single suppressive dose of combined sulphadoxine-pyrimethamine given the following month was 88.7%. No serious side effects were observed."}
{"id": "PMID:1092275", "title": "Liver disease in rheumatoid arthritis and Sj\u00f8gren's syndrome. Prospective study using biochemical and serological markers of hepatic dysfunction.", "content": "Inter-relationships of biochemical and immunological tests of liver function have been studied in a prospective study of 216 patients with rheumatoid arthritis (RA), 32 patients with Sjogren's syndrome, and 27 patients with the sicca syndrome, and these results have been compared with those obtained 289 patients with osteoarthrosis or with a form of seronegative polyarthropathy. In general the prevalence of abnormalities in serum alkaline phosphatase, bromsulphthalein excretion, smooth muscle antibody, and mitochondrial antibody in the former three groups was higher than in patients with osteoarthrosis. Patients with Sjogren's syndrome with RA had a higher prevalence of abnormalities of bromsulphthalein excretion, salivary duct antibody than patients with the sicca syndrome. Patients with RA had a higher pervalence of rheumatoid factor than those with the sicca syndrome. Patients with a positive smooth muscle or mitochondrial antibody were found to have a higher prevalence of hepatomegaly and splenomegaly, of abnormal liver function tests, of other autoantibodies, and of histological abnromalitis of liver than those in whom these tests were negative.", "contents": "Liver disease in rheumatoid arthritis and Sj\u00f8gren's syndrome. Prospective study using biochemical and serological markers of hepatic dysfunction. Inter-relationships of biochemical and immunological tests of liver function have been studied in a prospective study of 216 patients with rheumatoid arthritis (RA), 32 patients with Sjogren's syndrome, and 27 patients with the sicca syndrome, and these results have been compared with those obtained 289 patients with osteoarthrosis or with a form of seronegative polyarthropathy. In general the prevalence of abnormalities in serum alkaline phosphatase, bromsulphthalein excretion, smooth muscle antibody, and mitochondrial antibody in the former three groups was higher than in patients with osteoarthrosis. Patients with Sjogren's syndrome with RA had a higher prevalence of abnormalities of bromsulphthalein excretion, salivary duct antibody than patients with the sicca syndrome. Patients with RA had a higher pervalence of rheumatoid factor than those with the sicca syndrome. Patients with a positive smooth muscle or mitochondrial antibody were found to have a higher prevalence of hepatomegaly and splenomegaly, of abnormal liver function tests, of other autoantibodies, and of histological abnromalitis of liver than those in whom these tests were negative."}
{"id": "PMID:1092278", "title": "Metrifonate in urinary schistosomiasis. A field trial in northern Nigeria.", "content": "A field trial of metrifonate in the form of Bilarcil tablets (Bayer) was carried out in 39 northern Nigerian schoolchildren heavily infected with Schistosoma haematobium. Three oral doses of 7.5 mg/kg were given at four-week intervals. The drug proved effective (mean reduction in egg counts eight weeks after completion of treatment 94.5%) and was excellently tolerated. Its use on a larger scale is suggested.", "contents": "Metrifonate in urinary schistosomiasis. A field trial in northern Nigeria. A field trial of metrifonate in the form of Bilarcil tablets (Bayer) was carried out in 39 northern Nigerian schoolchildren heavily infected with Schistosoma haematobium. Three oral doses of 7.5 mg/kg were given at four-week intervals. The drug proved effective (mean reduction in egg counts eight weeks after completion of treatment 94.5%) and was excellently tolerated. Its use on a larger scale is suggested."}
{"id": "PMID:1092279", "title": "Management of acute aortic dissection.", "content": "The therapy for acute dissecting aneurysm of the aorta remains a difficult problem for thoracic surgeons. Because of an excessive operative mortality in patients with acute dissection who were operated on within 24 hours of hospital admission, we have utilized intensive medical management to delay surgical intervention. Even patients with acute aortic insufficiency can be supported medically, allowing their operations to be delayed at least 3 weeks or longer. Since this policy has been implemented, there has been no operative mortality in our last 13 patients with acute dissection. Medical therapy as the definitive treatment is now reserved solely for Type III dissections or for patients who cannot be operated on for other reasons. This report outlines our rationale for therapy and our current method of managing acute dissection.", "contents": "Management of acute aortic dissection. The therapy for acute dissecting aneurysm of the aorta remains a difficult problem for thoracic surgeons. Because of an excessive operative mortality in patients with acute dissection who were operated on within 24 hours of hospital admission, we have utilized intensive medical management to delay surgical intervention. Even patients with acute aortic insufficiency can be supported medically, allowing their operations to be delayed at least 3 weeks or longer. Since this policy has been implemented, there has been no operative mortality in our last 13 patients with acute dissection. Medical therapy as the definitive treatment is now reserved solely for Type III dissections or for patients who cannot be operated on for other reasons. This report outlines our rationale for therapy and our current method of managing acute dissection."}
{"id": "PMID:1092280", "title": "Calcified postoperative epicardial granuloma.", "content": "A sterile calcified granuloma on the external surface of a right ventriculotomy in an 8-year-old boy was identified four years after closure of a ventricular septal defect; He had no symptoms, but a systolic ejection murmur was identified and disappeared after resection of the lesion. Sensitivity to silk suture material is suggested by the operative findings and a history of multiple silk suture sinuses developing in his uninfected original thoracotomy incision.", "contents": "Calcified postoperative epicardial granuloma. A sterile calcified granuloma on the external surface of a right ventriculotomy in an 8-year-old boy was identified four years after closure of a ventricular septal defect; He had no symptoms, but a systolic ejection murmur was identified and disappeared after resection of the lesion. Sensitivity to silk suture material is suggested by the operative findings and a history of multiple silk suture sinuses developing in his uninfected original thoracotomy incision."}
{"id": "PMID:1092281", "title": "Overview of recent research in depression. Integration of ten conceptual models into a comprehensive clinical frame.", "content": "Disciplinary fragmentation and nosological and semantic controversies have obscured the impressive advances made in the area of depressive disorders during the past decade. This article is an attempt to translate data derived from psychodynamic, sociobehavioral, and neurobiologic research into a clinically meaningful framework. We review ten models of depression with special emphasis on newer models supported by empirical and experimental studies, and present a new model, which incorporates and synthesizes findings from different schools. Depressive illness is conceptualized as the feedback interaction of three sets of variables at chemical, experiential, and behavioral levels with the diencephalon serving as the field of action.", "contents": "Overview of recent research in depression. Integration of ten conceptual models into a comprehensive clinical frame. Disciplinary fragmentation and nosological and semantic controversies have obscured the impressive advances made in the area of depressive disorders during the past decade. This article is an attempt to translate data derived from psychodynamic, sociobehavioral, and neurobiologic research into a clinically meaningful framework. We review ten models of depression with special emphasis on newer models supported by empirical and experimental studies, and present a new model, which incorporates and synthesizes findings from different schools. Depressive illness is conceptualized as the feedback interaction of three sets of variables at chemical, experiential, and behavioral levels with the diencephalon serving as the field of action."}
{"id": "PMID:1092282", "title": "Interaction of drug therapy with marital therapy in depressive patients.", "content": "Comparative effects of the antidepressant drug, amitriptyline hydrochloride, marital therapy, and the drug-psychotherapy interaction effects were studied in a 12-week course of treatment of outpatient depressives. Patients were assigned randomly to four treatment groups, in a 2 times 2 factorial design: (1) drug-marital therapy; (2) drug-minimal contact; (3) placebo-marital therapy; and (4) placebo-minimal contact. Both drug and marital therapy showed substantial beneficial advantages over their control conditions, but drug therapy was faster and generally superior in symptom relief and clinical improvement. Marital therapy was superior in family role task performance and perception of the marital relationship. For reducing hostility and enhancing the perception of the marital relationship, drug therapy had a better early effect, but marital therapy had superior effects by the end of treatment.", "contents": "Interaction of drug therapy with marital therapy in depressive patients. Comparative effects of the antidepressant drug, amitriptyline hydrochloride, marital therapy, and the drug-psychotherapy interaction effects were studied in a 12-week course of treatment of outpatient depressives. Patients were assigned randomly to four treatment groups, in a 2 times 2 factorial design: (1) drug-marital therapy; (2) drug-minimal contact; (3) placebo-marital therapy; and (4) placebo-minimal contact. Both drug and marital therapy showed substantial beneficial advantages over their control conditions, but drug therapy was faster and generally superior in symptom relief and clinical improvement. Marital therapy was superior in family role task performance and perception of the marital relationship. For reducing hostility and enhancing the perception of the marital relationship, drug therapy had a better early effect, but marital therapy had superior effects by the end of treatment."}
{"id": "PMID:1092283", "title": "Antidepressants in black and white inpatients. Differential response to a controlled trial of chlorpromazine and imipramine.", "content": "Differential effects of chlorpromazine, imipramine hydrochloride, and a placebo were examined in 159 black and 555 white depressed patients in a multihospital collaborative study. In making these comparisons, the effects of age and social class were controlled. The major study findings were the differential effects of the active drugs for the black men and women. Chlorpromazine was the most efficacious treatment for black women, whereas imipramine was most efficacious for black men. These differences occurred on global ratings of improvement as well as on specific symptoms such as depression, anxiety, guilt-worthlessness, sleep disturbances, and social participation. Black patients also evidenced a higher improvement rate at one week, irrespective of treatment, than did the white patients.", "contents": "Antidepressants in black and white inpatients. Differential response to a controlled trial of chlorpromazine and imipramine. Differential effects of chlorpromazine, imipramine hydrochloride, and a placebo were examined in 159 black and 555 white depressed patients in a multihospital collaborative study. In making these comparisons, the effects of age and social class were controlled. The major study findings were the differential effects of the active drugs for the black men and women. Chlorpromazine was the most efficacious treatment for black women, whereas imipramine was most efficacious for black men. These differences occurred on global ratings of improvement as well as on specific symptoms such as depression, anxiety, guilt-worthlessness, sleep disturbances, and social participation. Black patients also evidenced a higher improvement rate at one week, irrespective of treatment, than did the white patients."}
{"id": "PMID:1092284", "title": "An adrenergic drug in depression.", "content": "It has been suggested that depression is a disease of cholinergic dominance and since the beta-adrenergic blocking drug propranolol hydrochloride can cause depression, there arises the possibility that a beta-adrenergic stimulant could benefit the condition. For ethical reasons, the adrenergic drug metaproterenol sulfate was combined with chlordiazepoxide hydrochloride and compared to placebo and chlordiazepoxide in a formal double-blind trial. However, the results did not show any advantage for the addition of metaproterenol either in respect of enhanced antidepressant effect or a reduced incidence, nature, or severity of side-effects.", "contents": "An adrenergic drug in depression. It has been suggested that depression is a disease of cholinergic dominance and since the beta-adrenergic blocking drug propranolol hydrochloride can cause depression, there arises the possibility that a beta-adrenergic stimulant could benefit the condition. For ethical reasons, the adrenergic drug metaproterenol sulfate was combined with chlordiazepoxide hydrochloride and compared to placebo and chlordiazepoxide in a formal double-blind trial. However, the results did not show any advantage for the addition of metaproterenol either in respect of enhanced antidepressant effect or a reduced incidence, nature, or severity of side-effects."}
{"id": "PMID:1092285", "title": "Double-blind comparison of lithium carbonate and imipramine in treatment of depression.", "content": "The effect of lithium carbonate therapy on patients with depression is still unconfirmed. Our past studies have shown a favorable response to the drug in patients with depression of mild or moderate severity. Therefore, we performed a controlled double-blind study of lithium carbonate and imipramine hydrochloride in 64 patients with depression. No significant differences were noted in the overall therapeutic response, depression scale scores, or clinical effects between the two drug groups.", "contents": "Double-blind comparison of lithium carbonate and imipramine in treatment of depression. The effect of lithium carbonate therapy on patients with depression is still unconfirmed. Our past studies have shown a favorable response to the drug in patients with depression of mild or moderate severity. Therefore, we performed a controlled double-blind study of lithium carbonate and imipramine hydrochloride in 64 patients with depression. No significant differences were noted in the overall therapeutic response, depression scale scores, or clinical effects between the two drug groups."}
{"id": "PMID:1092290", "title": "[The alumina oxide ceramic-metal composite endoprosthesis. A new hip endoprosthesis for partially cement free implantation (author's transl)].", "content": "The favorable combination of properties of dense, pure Al2O3-ceramics as a material for endoprostheses of the high load bearing joints have been confirmed in more than 4 years of experimental work with different types of animals. During this period Al2O3-ceramic parts for total hip replacement have already been developed and tested as well as the special instrumentation and the technique of the surgical procedure concerned. Based on these experiences a new total hip endoprostheses together with its complete technique for the operation is presented with which an improved alternative for replacements of Weber-Huggler-prostheses is offered and the application of total hip replacements can be extended to patients below 60 years of age.", "contents": "[The alumina oxide ceramic-metal composite endoprosthesis. A new hip endoprosthesis for partially cement free implantation (author's transl)]. The favorable combination of properties of dense, pure Al2O3-ceramics as a material for endoprostheses of the high load bearing joints have been confirmed in more than 4 years of experimental work with different types of animals. During this period Al2O3-ceramic parts for total hip replacement have already been developed and tested as well as the special instrumentation and the technique of the surgical procedure concerned. Based on these experiences a new total hip endoprostheses together with its complete technique for the operation is presented with which an improved alternative for replacements of Weber-Huggler-prostheses is offered and the application of total hip replacements can be extended to patients below 60 years of age."}
{"id": "PMID:1092291", "title": "Isolation of the spirochetes in the perilymph despite prior antisyphilitic therapy: a case report.", "content": "For the congenital syphilitic patient to develop sensorineural deafness is uncommon. For the congenital syphilitic patient to have persistence of treponemes after treatment is rare. To demonstrate viable organisms in the inner ear perilymph of the congenital syphilitic patient, despite treatment with penicillin, has not been recorded to date. This is a case report of such a patient.", "contents": "Isolation of the spirochetes in the perilymph despite prior antisyphilitic therapy: a case report. For the congenital syphilitic patient to develop sensorineural deafness is uncommon. For the congenital syphilitic patient to have persistence of treponemes after treatment is rare. To demonstrate viable organisms in the inner ear perilymph of the congenital syphilitic patient, despite treatment with penicillin, has not been recorded to date. This is a case report of such a patient."}
{"id": "PMID:1092292", "title": "Misadventures with injectable polytef (Teflon).", "content": "The intracordal injection of polytef (Teflon)-glycerine suspension has proved most effective in relieving dysphonia due to unilateral recurrent nerve paralysis. However, if errors in the technique of injection are committed or if patients are not properly selected, a potentially good result will not be achieved or the voice will be made worse.", "contents": "Misadventures with injectable polytef (Teflon). The intracordal injection of polytef (Teflon)-glycerine suspension has proved most effective in relieving dysphonia due to unilateral recurrent nerve paralysis. However, if errors in the technique of injection are committed or if patients are not properly selected, a potentially good result will not be achieved or the voice will be made worse."}
{"id": "PMID:1092293", "title": "Accuracy of the Bing and Rinne tuning fork tests.", "content": "Fifty children seen in an ENT clinic received the Bing and Rinne tuning fork tests, otoscopic examination, and puretone audiometry to determine the accuracy of the tuning fork tests in predicting otoscopic abnormality or conductive hearing loss. Neither tuning fork test showed high accuracy. The Bing test overidentified by yielding negative results (conductive loss) for many ears with normal hearing or sensorineural loss. It correctly identified conductive loss or otoscopic abnormality only slightly better than chance57% to 66% of the time. The Rinne test identified with high accuracy those ears with a 40 dB or greater air-bone gap. For air-bone gaps of 10-35 dB, only 27% of the ears gave negative results, meaning correct identification. However, when a negative Rinne occurred, the result was in error only 2% of the time.", "contents": "Accuracy of the Bing and Rinne tuning fork tests. Fifty children seen in an ENT clinic received the Bing and Rinne tuning fork tests, otoscopic examination, and puretone audiometry to determine the accuracy of the tuning fork tests in predicting otoscopic abnormality or conductive hearing loss. Neither tuning fork test showed high accuracy. The Bing test overidentified by yielding negative results (conductive loss) for many ears with normal hearing or sensorineural loss. It correctly identified conductive loss or otoscopic abnormality only slightly better than chance57% to 66% of the time. The Rinne test identified with high accuracy those ears with a 40 dB or greater air-bone gap. For air-bone gaps of 10-35 dB, only 27% of the ears gave negative results, meaning correct identification. However, when a negative Rinne occurred, the result was in error only 2% of the time."}
{"id": "PMID:1092294", "title": "Effects of injected deposteroid on posttonsillectomy morbidity: a double-blind study.", "content": "There are numerous studies using various agents to reduce posttonsillectomy morbidity. Due to lack of conclusive results a double-blind study using methylprednisolone acetate (Depo Medrol) was conducted. A total of 165 patients were randomly divided into two groups. Following tonsillectomy 2.5 ml of solution was injected into the base of each tonsillar fossa. The study group received 20 mg of deposteroid to each fossa while the control group got normal saline. The deposteroid reduced postoperative pain but did not significantly alter other factors contributing to morbidity such as difficulty in swallowing or resumption of a normal diet. The deposteroid appeared to have no effect on the rate of healing of the tonsillar fossa.", "contents": "Effects of injected deposteroid on posttonsillectomy morbidity: a double-blind study. There are numerous studies using various agents to reduce posttonsillectomy morbidity. Due to lack of conclusive results a double-blind study using methylprednisolone acetate (Depo Medrol) was conducted. A total of 165 patients were randomly divided into two groups. Following tonsillectomy 2.5 ml of solution was injected into the base of each tonsillar fossa. The study group received 20 mg of deposteroid to each fossa while the control group got normal saline. The deposteroid reduced postoperative pain but did not significantly alter other factors contributing to morbidity such as difficulty in swallowing or resumption of a normal diet. The deposteroid appeared to have no effect on the rate of healing of the tonsillar fossa."}
{"id": "PMID:1092295", "title": "Silicone elastomer sheeting in the development of pedicle flaps.", "content": "A \"delay\" of a pedicle flap stimulates improvement in the flap circulation, probably through partial ischemia. The degree of stimulation prsent at any given time is inversely proportional to the length of time that has passed after the delay because progressive revascularization across the delay lines reduces the ischemis stimulus. This revascularization can ve prevented by placing silicone elastomers (Silastic) sheeting between the flap and its bed.", "contents": "Silicone elastomer sheeting in the development of pedicle flaps. A \"delay\" of a pedicle flap stimulates improvement in the flap circulation, probably through partial ischemia. The degree of stimulation prsent at any given time is inversely proportional to the length of time that has passed after the delay because progressive revascularization across the delay lines reduces the ischemis stimulus. This revascularization can ve prevented by placing silicone elastomers (Silastic) sheeting between the flap and its bed."}
{"id": "PMID:1092297", "title": "[Influence of hypothyreodism on the quantitative Karyo- and cytoarchitecture of rat hepatocytes. (A morphometrical and cytophotometrical study on rat hepatocytes 3 weeks after thyreoidectomy) (author's transl)].", "content": "Thyroid hormoned influence the quantitative cytoarchitecture of hepatocytes by stimulating the membrane synthesis of mitochondria and ergastoplasm. The cellular and nuclear changes produced by thyroidectomy were analysed cytophotometrically and morphometrically. 21 days after thyroidectomy the liver tissues of 10 male Wistar rats were examined. 5 sham-operated animals served as controls. All rats received Altromin-R-standard diet. The cytophotometric analysis is based on the method described by Sandritter. The morphometric study was carried out following Weibel's methods. As a consequence of the changed cellular metabolism, smaller hepatocytes and nuclei (but larger nucleoli) are found in the hypothyroid rats. As compared with controls, hepatocytes hardly show tetraploid nuclei, which is due to inhibited polyploidisation. The mitochondrial content has become considerably smaller in the hypothyroid hepatocytes. The cristal membranes, the mitochondrial and peroxysomal volume per hepatocyte show a similar reaction pattern, which is considered to be a sign of reduced cellular metabolism. The volume loss of the ergastoplasm confirms this assumption.", "contents": "[Influence of hypothyreodism on the quantitative Karyo- and cytoarchitecture of rat hepatocytes. (A morphometrical and cytophotometrical study on rat hepatocytes 3 weeks after thyreoidectomy) (author's transl)]. Thyroid hormoned influence the quantitative cytoarchitecture of hepatocytes by stimulating the membrane synthesis of mitochondria and ergastoplasm. The cellular and nuclear changes produced by thyroidectomy were analysed cytophotometrically and morphometrically. 21 days after thyroidectomy the liver tissues of 10 male Wistar rats were examined. 5 sham-operated animals served as controls. All rats received Altromin-R-standard diet. The cytophotometric analysis is based on the method described by Sandritter. The morphometric study was carried out following Weibel's methods. As a consequence of the changed cellular metabolism, smaller hepatocytes and nuclei (but larger nucleoli) are found in the hypothyroid rats. As compared with controls, hepatocytes hardly show tetraploid nuclei, which is due to inhibited polyploidisation. The mitochondrial content has become considerably smaller in the hypothyroid hepatocytes. The cristal membranes, the mitochondrial and peroxysomal volume per hepatocyte show a similar reaction pattern, which is considered to be a sign of reduced cellular metabolism. The volume loss of the ergastoplasm confirms this assumption."}
{"id": "PMID:1092313", "title": "Further investigations on M. leprae. Growth promotion and inhibition by organic substances and observations on antagonistic and syntergistic effects.", "content": "The multiplication of 2 strains of M. leprae on a medium containing a sonic extract (SE), prepared from M. smegmatis, was promoted by cysteine, tryptophane and dimethylasulfoxide (DMSO), while glutamic acid, glutamine and histidine exerted variable effects.  The final effects of glutamic acid and glutamine were determined by the total concentration of both compounds together.  The presence of cysteine and glutamic acid alone or together with DMSO abolished all inhibitory effects.  Desferal did not enable the multiplication of M. leprae on media devoid of SE prepared from M. smegmatis.  However with SE and 0.005 per cent and 0.002 per cent concentrations of Desferal its initial growth was accelerated.  Its final counts, noted after an 8-month incubation, did not exceed those observed without Desferal.  Puring and pyrimidine compounds promoted markekly the multiplication of M. leprae (counts greater than 3 times 10-7/ml).  The highest counts were observed with pyrimidines (thymine, thymidine, cytosine) applied single or combined.", "contents": "Further investigations on M. leprae. Growth promotion and inhibition by organic substances and observations on antagonistic and syntergistic effects. The multiplication of 2 strains of M. leprae on a medium containing a sonic extract (SE), prepared from M. smegmatis, was promoted by cysteine, tryptophane and dimethylasulfoxide (DMSO), while glutamic acid, glutamine and histidine exerted variable effects.  The final effects of glutamic acid and glutamine were determined by the total concentration of both compounds together.  The presence of cysteine and glutamic acid alone or together with DMSO abolished all inhibitory effects.  Desferal did not enable the multiplication of M. leprae on media devoid of SE prepared from M. smegmatis.  However with SE and 0.005 per cent and 0.002 per cent concentrations of Desferal its initial growth was accelerated.  Its final counts, noted after an 8-month incubation, did not exceed those observed without Desferal.  Puring and pyrimidine compounds promoted markekly the multiplication of M. leprae (counts greater than 3 times 10-7/ml).  The highest counts were observed with pyrimidines (thymine, thymidine, cytosine) applied single or combined."}
{"id": "PMID:1092320", "title": "Effect of propranolol on left ventricular function, segmental wall motion, and diastolic pressure-volume relation in man.", "content": "Precise quantitation of the effects of the non-selective beta adrenergic blocking drug propranolol (3.15 mg/kg body weight) on left ventricular function, segmental wall motion, and diastolic pressure-volume relation in man has been performed. High fidelity left ventricular pressure measurements and simultaneous single-plane angiocardiograms were recorded on a video disc and volumes calculated by a light-pen computer system. Systolic segmental wall motion was computer analysed using the long axis-quadrasection method. Patients were transvenously atrially paced to maintain a constant heart rate. The haemodynamic effects of propranolol may vary depending upon the extent of pre-existing myocardial disease. In some patients ventricular function, as measured by ejection fraction, may be reduced. This reduction in ejection fraction appears to result from overall reduction in segmental wall motion, but also from accentuation of segmental wall abnormalities. These results are consistent with the thesis that beta adrenergic blocking drugs may inhibit compensatory sympathetic mechanisms. The diastolic effects of propranolol may include quite substantial increases in ventricular volumes in those patients with impaired cardiac function. With respect to the intact human ventricle, propranolol may increase diastolic volume for a given level of ventricular pressure. Thus, in a static sense, the ventricle in these patients could be viewed as being more compliant after propranolol administration. However, the fact that the length-tension relation, as measured by the slope of the logarithmic pressure versus volume plot is unaltered by propranolol, suggests that the muscle comprising the ventricle itself exhibits no alteration in its passive elastic properties.", "contents": "Effect of propranolol on left ventricular function, segmental wall motion, and diastolic pressure-volume relation in man. Precise quantitation of the effects of the non-selective beta adrenergic blocking drug propranolol (3.15 mg/kg body weight) on left ventricular function, segmental wall motion, and diastolic pressure-volume relation in man has been performed. High fidelity left ventricular pressure measurements and simultaneous single-plane angiocardiograms were recorded on a video disc and volumes calculated by a light-pen computer system. Systolic segmental wall motion was computer analysed using the long axis-quadrasection method. Patients were transvenously atrially paced to maintain a constant heart rate. The haemodynamic effects of propranolol may vary depending upon the extent of pre-existing myocardial disease. In some patients ventricular function, as measured by ejection fraction, may be reduced. This reduction in ejection fraction appears to result from overall reduction in segmental wall motion, but also from accentuation of segmental wall abnormalities. These results are consistent with the thesis that beta adrenergic blocking drugs may inhibit compensatory sympathetic mechanisms. The diastolic effects of propranolol may include quite substantial increases in ventricular volumes in those patients with impaired cardiac function. With respect to the intact human ventricle, propranolol may increase diastolic volume for a given level of ventricular pressure. Thus, in a static sense, the ventricle in these patients could be viewed as being more compliant after propranolol administration. However, the fact that the length-tension relation, as measured by the slope of the logarithmic pressure versus volume plot is unaltered by propranolol, suggests that the muscle comprising the ventricle itself exhibits no alteration in its passive elastic properties."}
{"id": "PMID:1092321", "title": "Functional and post-oral contraceptive amenorrhoea: response to luteinizing hormone releasing hormone (LH-RH).", "content": "Basal levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in serum and their response to the luteinizing hormone releasing hormone (LH-RH) were measured in a group of patients with functional amenorrhoea. The results in this group were compared to those obtained in a second group of patients with secondary amenorrhoea which had followed cessation of a combined oral contraceptive. No difference either in the basal levels or in the response to LH-RH could be found. These data indicate that there is no detectable difference in pituitary gonadotrophin status between the two groups and that the two types of amenorrhoea have ghe same aetiological background.", "contents": "Functional and post-oral contraceptive amenorrhoea: response to luteinizing hormone releasing hormone (LH-RH). Basal levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) in serum and their response to the luteinizing hormone releasing hormone (LH-RH) were measured in a group of patients with functional amenorrhoea. The results in this group were compared to those obtained in a second group of patients with secondary amenorrhoea which had followed cessation of a combined oral contraceptive. No difference either in the basal levels or in the response to LH-RH could be found. These data indicate that there is no detectable difference in pituitary gonadotrophin status between the two groups and that the two types of amenorrhoea have ghe same aetiological background."}
{"id": "PMID:1092322", "title": "Pathological mechanisms to explain some cases of amenorrhoea without organic disease.", "content": "A test is described to define the site of the lesion causing amenorrhoea in women of child-bearing age who have normal ovaries and a normal response to luteinizing hormone-releasing hormone (LH-RH). Patients able to produce a release of LH following the administration of 1 mg. of oestradiol benzoate (EB) have normal hypothalamic function and thus the site of the defect must lie more centrally in the brain. Patients unable to show these LH surges have either a primary or secondary abnormality affecting their hypothalamic oestrogen \"receptor\" mechanisms or their ability to produce LH-RH.", "contents": "Pathological mechanisms to explain some cases of amenorrhoea without organic disease. A test is described to define the site of the lesion causing amenorrhoea in women of child-bearing age who have normal ovaries and a normal response to luteinizing hormone-releasing hormone (LH-RH). Patients able to produce a release of LH following the administration of 1 mg. of oestradiol benzoate (EB) have normal hypothalamic function and thus the site of the defect must lie more centrally in the brain. Patients unable to show these LH surges have either a primary or secondary abnormality affecting their hypothalamic oestrogen \"receptor\" mechanisms or their ability to produce LH-RH."}
{"id": "PMID:1092324", "title": "Translation of poly(riboadenylic acid)-enriched messenger RNAs from the yeast, Saccharomyces cerevisiae, in heterologous cell-free systems.", "content": "Poly(riboadenylic acid) [poly(A)] enriched messenger RNAs from yeast have been used to direct the synthesis of yeast polypeptides in mouse Krebs II ascites and wheat embryo extracts. Both cell-free systems, synthesize polypeptides over a molecular weight range of 10,000-100,000. Autoradiograms of sodium dodecyl sulfate-polyacrylamide slab gels used to fractionate [35S]methionine-labeled in vitro products reveal that about 25 major bands (each of them possibly representing multiple polypeptides) are produced by each cell-free system. Each of these coelectrophoreses with a major polypeptide labeled in vivo or in a yeast lysate. These results suggest that cell-free translational machinery from eukaryotes is not able to discriminate in an all or none fashion against messenger RNAs which are available to it. While yeast poly(A)-enriched messenger RNA directs the synthesis polypeptides over approximately the same molecular weight range in both cell-free systems, the wheat germ system directs the incorporation of 45 times the amount of [3H]serine into Cl3CCOOH-precipitable polypeptides. This is in contrast to the 2.5-fold more efficient translation of hemoglobin mRNA in the wheat embryo extract. Thus, the extract from mammalian cells is able to translate mRNA from a lower plant with a much lower efficiency than it translates hemoglobin mRNA, and at a lower efficiency than is observed using a cell-free system from wheat embryos. This indicates that the wheat embryo system is the one of choice for translation of yeast messenger RNA.", "contents": "Translation of poly(riboadenylic acid)-enriched messenger RNAs from the yeast, Saccharomyces cerevisiae, in heterologous cell-free systems. Poly(riboadenylic acid) [poly(A)] enriched messenger RNAs from yeast have been used to direct the synthesis of yeast polypeptides in mouse Krebs II ascites and wheat embryo extracts. Both cell-free systems, synthesize polypeptides over a molecular weight range of 10,000-100,000. Autoradiograms of sodium dodecyl sulfate-polyacrylamide slab gels used to fractionate [35S]methionine-labeled in vitro products reveal that about 25 major bands (each of them possibly representing multiple polypeptides) are produced by each cell-free system. Each of these coelectrophoreses with a major polypeptide labeled in vivo or in a yeast lysate. These results suggest that cell-free translational machinery from eukaryotes is not able to discriminate in an all or none fashion against messenger RNAs which are available to it. While yeast poly(A)-enriched messenger RNA directs the synthesis polypeptides over approximately the same molecular weight range in both cell-free systems, the wheat germ system directs the incorporation of 45 times the amount of [3H]serine into Cl3CCOOH-precipitable polypeptides. This is in contrast to the 2.5-fold more efficient translation of hemoglobin mRNA in the wheat embryo extract. Thus, the extract from mammalian cells is able to translate mRNA from a lower plant with a much lower efficiency than it translates hemoglobin mRNA, and at a lower efficiency than is observed using a cell-free system from wheat embryos. This indicates that the wheat embryo system is the one of choice for translation of yeast messenger RNA."}
{"id": "PMID:1092325", "title": "The irreversible step in formation of initiation complexes of Escherichia coli.", "content": "At some stage during initiation the ribosomal subunits of Escherichia coli must become irreversibly coupled, since polysomal ribosomes, in contrast to free ribosomes, are not dissociated by initiation factor IF-3. To determine when irreversibility develops we have compared the response to IF-3 of mature, puromycin-reactive initiation complexes, made with GTP, and of intermediate, puromycin-unreactive complexes, made with GMPPCP. The latter complexes initially appeared to be dissociated by the factor but this effect was found to be due to artificial loss of the ligands at the Mg2+ concentration customary in the test for dissociation. At a slightly higher Mg2+ concentration (4 mM), sufficient to retain the ligands, the GMPPCP complexes were not significantly dissociated by IF-3, at concentrations that caused complex dissociation of free ribosomes. It thus appears that the intermediate 70S initiation complex, though less stable to ionic dissociation than the mature complex, is in effect irreversible under physiological conditions.", "contents": "The irreversible step in formation of initiation complexes of Escherichia coli. At some stage during initiation the ribosomal subunits of Escherichia coli must become irreversibly coupled, since polysomal ribosomes, in contrast to free ribosomes, are not dissociated by initiation factor IF-3. To determine when irreversibility develops we have compared the response to IF-3 of mature, puromycin-reactive initiation complexes, made with GTP, and of intermediate, puromycin-unreactive complexes, made with GMPPCP. The latter complexes initially appeared to be dissociated by the factor but this effect was found to be due to artificial loss of the ligands at the Mg2+ concentration customary in the test for dissociation. At a slightly higher Mg2+ concentration (4 mM), sufficient to retain the ligands, the GMPPCP complexes were not significantly dissociated by IF-3, at concentrations that caused complex dissociation of free ribosomes. It thus appears that the intermediate 70S initiation complex, though less stable to ionic dissociation than the mature complex, is in effect irreversible under physiological conditions."}
{"id": "PMID:1092326", "title": "The predicted secondary structure of the N-terminal sequence of the lac repressor and proposed models for its complexation to the lac operator.", "content": "Rules for the prediction of protein conformation (Chou, P. Y., and Fasman, G. D. (1974), Biochemistry 13, 211, 222) have been applied to the N-terminal sequence 1-60 of the lac repressor. This analysis predicts beta structure at sequences 4-9 and 15-20, helices at 26-32, 38-45, and 52-57, and beta turns at 48-51 and 14-17. Repressor mutants lacking operator binding capacity in which Pro replaces Ser-16 and Ala replaces Thr-19 (Weber, K., Platt, T., Ganem, D., and Miller, J. H. (1972), Proc. Natl. Acad. Sci. U.S.A. 69, 3624) have no effect on the prediction of beta structure at residues 15 to 20, which suggests that the polar side chains of Ser-16, Tyr-17, Gln-18, and Thr-19 participate in intermolecular hydrogen bonding with conplementary polar groups on the lac operator. The loss of operator binding capacity on replacement of Ala by Val at position 53 in the repressor results from a predicted secondary structural change from helix to beta structure for residues 52-57 which can be transmitted to the N-terminal sequence via a beta turn at residues 48-51. The basic residues at positions 33, 35, and 37 between the helical regions 26-32 and 38-45 probably bind to the phosphate groups on the operator on complexation. It is proposed that complex formation involves the interaction of either a beta structure (residues 15-20) or a right-hand twisted antiparallel beta-pleated sheet (residues 4-9 and 12-20) with operator DNA.", "contents": "The predicted secondary structure of the N-terminal sequence of the lac repressor and proposed models for its complexation to the lac operator. Rules for the prediction of protein conformation (Chou, P. Y., and Fasman, G. D. (1974), Biochemistry 13, 211, 222) have been applied to the N-terminal sequence 1-60 of the lac repressor. This analysis predicts beta structure at sequences 4-9 and 15-20, helices at 26-32, 38-45, and 52-57, and beta turns at 48-51 and 14-17. Repressor mutants lacking operator binding capacity in which Pro replaces Ser-16 and Ala replaces Thr-19 (Weber, K., Platt, T., Ganem, D., and Miller, J. H. (1972), Proc. Natl. Acad. Sci. U.S.A. 69, 3624) have no effect on the prediction of beta structure at residues 15 to 20, which suggests that the polar side chains of Ser-16, Tyr-17, Gln-18, and Thr-19 participate in intermolecular hydrogen bonding with conplementary polar groups on the lac operator. The loss of operator binding capacity on replacement of Ala by Val at position 53 in the repressor results from a predicted secondary structural change from helix to beta structure for residues 52-57 which can be transmitted to the N-terminal sequence via a beta turn at residues 48-51. The basic residues at positions 33, 35, and 37 between the helical regions 26-32 and 38-45 probably bind to the phosphate groups on the operator on complexation. It is proposed that complex formation involves the interaction of either a beta structure (residues 15-20) or a right-hand twisted antiparallel beta-pleated sheet (residues 4-9 and 12-20) with operator DNA."}
{"id": "PMID:1092327", "title": "Binding of Met-tRNA-f to native and derived 40S ribosomal subunits.", "content": "Our previous work has shown that the native 40S ribosomal subunits (those found free in the cell sap) but not polyribosomal 40S subunits have additional associated proteins that are removed by 0.5 M KCl. In this communication we present evidence that in the Ehrlich cell one of the native subunit associated proteins is the mammalian initiation factor that forms a Met-tRNA-f-factor-GTP complex, and is required for the binding of Met-tRNA-f to the 40S subunit. Initial examination of the KCl wash of the Ehrlich cell total ribosomal pellet revealed a factor which (1) shifted the elution of Met-tRNA-f and of GTP from the included to the excluded volume on Sephadex G-100 chromatography, (2) stimulated the binding of Met-tRNA-f to Millipore filters, and (3) stimulated the binding of Met-tRNA-f to salt-washed 40S subunits. These activities were dependent upon or enhanced by GTP; were inhibited by GDP; were much greater for Met-tRNA-f than for Met-tRNA-m or for lysyl-tRNA; and were concentrated in the KCl ribosomal wash and were not detected in the cell soluble fraction. Met-tRNA-f bound in conjunction with a specific amount of KCl wash protein, to form a distinctive particle of bouyant density 1.40 g cm minus 3 in CsCl, identical in density to one form of the native 40S subunit. Native 40S subunits, but no other subunits, contained a factor which was eluted by 0.5 M KCl and which (1) stimulated the binding of Met-tRNA-f to Millipore filters, and (2) stimulated the binding of Met-tRNA-f to salt-washed 40S subunits. The factor appeared to be localized on the native 40S subunit of density 1.40 g cm minus 3.", "contents": "Binding of Met-tRNA-f to native and derived 40S ribosomal subunits. Our previous work has shown that the native 40S ribosomal subunits (those found free in the cell sap) but not polyribosomal 40S subunits have additional associated proteins that are removed by 0.5 M KCl. In this communication we present evidence that in the Ehrlich cell one of the native subunit associated proteins is the mammalian initiation factor that forms a Met-tRNA-f-factor-GTP complex, and is required for the binding of Met-tRNA-f to the 40S subunit. Initial examination of the KCl wash of the Ehrlich cell total ribosomal pellet revealed a factor which (1) shifted the elution of Met-tRNA-f and of GTP from the included to the excluded volume on Sephadex G-100 chromatography, (2) stimulated the binding of Met-tRNA-f to Millipore filters, and (3) stimulated the binding of Met-tRNA-f to salt-washed 40S subunits. These activities were dependent upon or enhanced by GTP; were inhibited by GDP; were much greater for Met-tRNA-f than for Met-tRNA-m or for lysyl-tRNA; and were concentrated in the KCl ribosomal wash and were not detected in the cell soluble fraction. Met-tRNA-f bound in conjunction with a specific amount of KCl wash protein, to form a distinctive particle of bouyant density 1.40 g cm minus 3 in CsCl, identical in density to one form of the native 40S subunit. Native 40S subunits, but no other subunits, contained a factor which was eluted by 0.5 M KCl and which (1) stimulated the binding of Met-tRNA-f to Millipore filters, and (2) stimulated the binding of Met-tRNA-f to salt-washed 40S subunits. The factor appeared to be localized on the native 40S subunit of density 1.40 g cm minus 3."}
{"id": "PMID:1092328", "title": "Physical properties of some ribosomal proteins in solution and evidence for molecular interactions between isolated ribosomal proteins.", "content": "Many previous studies have been directed toward obtaining a physical visualization of the relationship between the protein and RNA in the ribosomal subunits isolated from Escherichia coli. The current study is the first report where an attempt has been made to directly assess interactions between a pair of isolated ribosomal proteins separate from the intact system by means of sedimentation equilibrium analysis. The molecular weights of the proteins S3, S4, S5, S6, S7, S8, and S20 from the 30S subunit of the E. coli ribosome were determined under conditions of assembly of the subunit by sedimentation equilibrium. All of the proteins exhibited molecular weights consistent with monomeric behavior (i.e., in agreement with the measurement of the ultimate molecular weight in denaturing solvents as reported in other studies as well as in the current study) except S8 which indicates a tendency to self-associate. Hydrodynamic measurements on the proteins indicate that these proteins are not completely disorganized in solution such as a random coil, although not as compact as globular proteins. The frictional coefficient ratios found for these ribosomal proteins range from 1.4 to 1.9. The hydrodynamic data are discussed as containing some evidence that stable interaction sites could exist in the proteins. The molecular weight data are considered pertinent to a sedimentation equilibrium study of protein-protein interactions that may be occurring in the ribosomal subunits. Two proteins, S3 and S5, considered in this investigation were found to exhibit no tendency to self-associate under conditions of reassembly. When the two proteins are mixed under those same conditions, however, a species with a molecular weight greater than that of either S3 or S5 is observed to be formed. The interpretation is presented that a molecular interaction between S3 and S5 is the cause. The system is described as containing S3, S5, and a complex between S3 and S5 with a stoichiometry of 1:1 and an association equilibrium constant of 5.7 times 10-5 l./mol (delta G-o equals minus 7.25 kcal/mol). Since the association appears to be specific and of moderate strength, it is concluded that the interaction could have some pertinence with respect to conferring a structural arrangement in the ribosomal subunit. Moreover, it is concluded that protein-protein interactions, in general, must be considered in addition to the well documented significant RNA-protein relationships when models for ribosome structure and assembly are formulated.", "contents": "Physical properties of some ribosomal proteins in solution and evidence for molecular interactions between isolated ribosomal proteins. Many previous studies have been directed toward obtaining a physical visualization of the relationship between the protein and RNA in the ribosomal subunits isolated from Escherichia coli. The current study is the first report where an attempt has been made to directly assess interactions between a pair of isolated ribosomal proteins separate from the intact system by means of sedimentation equilibrium analysis. The molecular weights of the proteins S3, S4, S5, S6, S7, S8, and S20 from the 30S subunit of the E. coli ribosome were determined under conditions of assembly of the subunit by sedimentation equilibrium. All of the proteins exhibited molecular weights consistent with monomeric behavior (i.e., in agreement with the measurement of the ultimate molecular weight in denaturing solvents as reported in other studies as well as in the current study) except S8 which indicates a tendency to self-associate. Hydrodynamic measurements on the proteins indicate that these proteins are not completely disorganized in solution such as a random coil, although not as compact as globular proteins. The frictional coefficient ratios found for these ribosomal proteins range from 1.4 to 1.9. The hydrodynamic data are discussed as containing some evidence that stable interaction sites could exist in the proteins. The molecular weight data are considered pertinent to a sedimentation equilibrium study of protein-protein interactions that may be occurring in the ribosomal subunits. Two proteins, S3 and S5, considered in this investigation were found to exhibit no tendency to self-associate under conditions of reassembly. When the two proteins are mixed under those same conditions, however, a species with a molecular weight greater than that of either S3 or S5 is observed to be formed. The interpretation is presented that a molecular interaction between S3 and S5 is the cause. The system is described as containing S3, S5, and a complex between S3 and S5 with a stoichiometry of 1:1 and an association equilibrium constant of 5.7 times 10-5 l./mol (delta G-o equals minus 7.25 kcal/mol). Since the association appears to be specific and of moderate strength, it is concluded that the interaction could have some pertinence with respect to conferring a structural arrangement in the ribosomal subunit. Moreover, it is concluded that protein-protein interactions, in general, must be considered in addition to the well documented significant RNA-protein relationships when models for ribosome structure and assembly are formulated."}
{"id": "PMID:1092329", "title": "Interaction of steroids with nucleic acids.", "content": "17beta-Estradiol and testosterone bind to both native and denatured DNA, and to RNA and poly(A)-poly(U). Binding affinity depends on the conformation of nucleic acid. Lowering the electrolyte concentration and raising the temperature increase the binding of 17beta-estradiol to native DNA and decrease that to denatured DNA. In 0.01 M NaCl and at 37 degrees, more 17beta-estradiol is bound to native DNA than to denatured DNA. Higher binding of steroid to denatured DNA relative to native DNA at low temperature and high ionic strength is related to larger fraction of binding sites per unit nucleotide in denatured DNA. In addition to 17beta-estradiol and testosterone, 17alpha-estradiol, 17beta-estradiol-3-methyl ether and 19-nortestosterone also stabilize the structure of nucleic acids and poly(A)-poly(U) against thermal denaturation. The 17beta-estradiol induced elevation of the T-m of DNA is diminished by methanol or high NaCl concentration. These results indicate the involvement of hydrogen bonding and hydrophobic interactions between steroids and nucleic acids. The results of binding isotherms and optical studies suggest a conformational dependence of the binding of steroids to nucleic acids.", "contents": "Interaction of steroids with nucleic acids. 17beta-Estradiol and testosterone bind to both native and denatured DNA, and to RNA and poly(A)-poly(U). Binding affinity depends on the conformation of nucleic acid. Lowering the electrolyte concentration and raising the temperature increase the binding of 17beta-estradiol to native DNA and decrease that to denatured DNA. In 0.01 M NaCl and at 37 degrees, more 17beta-estradiol is bound to native DNA than to denatured DNA. Higher binding of steroid to denatured DNA relative to native DNA at low temperature and high ionic strength is related to larger fraction of binding sites per unit nucleotide in denatured DNA. In addition to 17beta-estradiol and testosterone, 17alpha-estradiol, 17beta-estradiol-3-methyl ether and 19-nortestosterone also stabilize the structure of nucleic acids and poly(A)-poly(U) against thermal denaturation. The 17beta-estradiol induced elevation of the T-m of DNA is diminished by methanol or high NaCl concentration. These results indicate the involvement of hydrogen bonding and hydrophobic interactions between steroids and nucleic acids. The results of binding isotherms and optical studies suggest a conformational dependence of the binding of steroids to nucleic acids."}
{"id": "PMID:1092330", "title": "Nucleotide specificity of stringent factor and the synthesis of analogs of guanosine 5'-diphosphate 3'-diphosphate and guanosine 5'-triphosphate 3'-diphosphate.", "content": "The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5'-monophosphate 3'-diphosphate and guanosine 5'-tetraphosphate 3'-diphosphate were the products of these reactions. Furthermore, 3'-linked analogs of guanosine 5'-diphosphate 3'-diphosphate and guanosine 5'-triphosphate 3'-diphosphate were synthesized from the corresponding ATP analog, adenosine 5'-O-(3-thiotriphosphate). The stringent factor catalyzed reverse reaction was found to be specific for guanosine 5'-diphosphate 3'-diphosphate, and was essentially inactive to the isomeric form, guanosine 5'-diphosphate 2'-diphosphate.", "contents": "Nucleotide specificity of stringent factor and the synthesis of analogs of guanosine 5'-diphosphate 3'-diphosphate and guanosine 5'-triphosphate 3'-diphosphate. The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5'-monophosphate 3'-diphosphate and guanosine 5'-tetraphosphate 3'-diphosphate were the products of these reactions. Furthermore, 3'-linked analogs of guanosine 5'-diphosphate 3'-diphosphate and guanosine 5'-triphosphate 3'-diphosphate were synthesized from the corresponding ATP analog, adenosine 5'-O-(3-thiotriphosphate). The stringent factor catalyzed reverse reaction was found to be specific for guanosine 5'-diphosphate 3'-diphosphate, and was essentially inactive to the isomeric form, guanosine 5'-diphosphate 2'-diphosphate."}
{"id": "PMID:1092331", "title": "The regulation of transcription in bacteriophage T5-infected Escherichia coli.", "content": "The expression of bacteriophage T5-specific RNA and protein in infected cells is temporally separated into three classes: class I (preearly), class II (early), and class III (late). By immunoprecipitation techniques we have shown that T5 infection of cells leads to the synthesis of one class I polypeptide (11,000 daltons) and two class II polypeptides (90,000 and 15,000 daltons) capable of binding to the RNA polymerase of the host Escherichia coli cell. One of the class II polypeptides (90,000 daltons) is the product of gene C2, which is an essential gene product required for the initiation of class III RNA synthesis. The colicinogenic factor, ColIb, is a plasmid which prevents the normal synthesis of class II and the III bacteriophage T5-specific RNA in infected colicinogenic (ColIb+) cells. In T5-infected colicinogenic cells, only the T5 class I polypeptide is found associated with the RNA polymerase. Mutants of T5, designated T5h minus, are capable of growth on both noncolicinogenic and ColIb+ hosts. Extracts of T5h minus infected ColIb+ cells were shown to lack a small class I polypeptide (12,000 daltons) as compared to T5-infected cells. The h minus mutation, however, has no effect on the levels of the class I T5 polypeptide of similar molecular weight which is bound to the RNA polymerase. One effect of the h minus mutation is to enhance the quantities of the two class II polypeptides bound to the enzyme.", "contents": "The regulation of transcription in bacteriophage T5-infected Escherichia coli. The expression of bacteriophage T5-specific RNA and protein in infected cells is temporally separated into three classes: class I (preearly), class II (early), and class III (late). By immunoprecipitation techniques we have shown that T5 infection of cells leads to the synthesis of one class I polypeptide (11,000 daltons) and two class II polypeptides (90,000 and 15,000 daltons) capable of binding to the RNA polymerase of the host Escherichia coli cell. One of the class II polypeptides (90,000 daltons) is the product of gene C2, which is an essential gene product required for the initiation of class III RNA synthesis. The colicinogenic factor, ColIb, is a plasmid which prevents the normal synthesis of class II and the III bacteriophage T5-specific RNA in infected colicinogenic (ColIb+) cells. In T5-infected colicinogenic cells, only the T5 class I polypeptide is found associated with the RNA polymerase. Mutants of T5, designated T5h minus, are capable of growth on both noncolicinogenic and ColIb+ hosts. Extracts of T5h minus infected ColIb+ cells were shown to lack a small class I polypeptide (12,000 daltons) as compared to T5-infected cells. The h minus mutation, however, has no effect on the levels of the class I T5 polypeptide of similar molecular weight which is bound to the RNA polymerase. One effect of the h minus mutation is to enhance the quantities of the two class II polypeptides bound to the enzyme."}
{"id": "PMID:1092332", "title": "Amino acid sequence of dogfish trypsin.", "content": "The amino acid sequence of pancreatic trypsin from the spiny Pacific dogfish (Squalus acanthias) has been determined and compared with the sequences of bovine and porcine trypsin. Dogfish trypsin contains one less amino acid residue (222) than the other two enzymes. Two-thirds of the residues in corresponding positions in dogfish and bovine trypsin are identical and the sequences ofall three enzymes are homologous. Of the 223 amino acid residues of bovine trypsin, 77 are replaced without significant changes in function. Seven replacements, all conservative, occur in the interior of the protein; the remainder are on the surface. All residues known to be components of the active site of bovine trypsin are present in corresponding positions in dogfish trypsin. Comparison of the three enzymes suggests calcium binding sites in dogfish trypsin. A corrected sequence of bovine trypsin identifies residue 67 as Asn and residues 84-87 as Ser-Asn-Thr-Leu.", "contents": "Amino acid sequence of dogfish trypsin. The amino acid sequence of pancreatic trypsin from the spiny Pacific dogfish (Squalus acanthias) has been determined and compared with the sequences of bovine and porcine trypsin. Dogfish trypsin contains one less amino acid residue (222) than the other two enzymes. Two-thirds of the residues in corresponding positions in dogfish and bovine trypsin are identical and the sequences ofall three enzymes are homologous. Of the 223 amino acid residues of bovine trypsin, 77 are replaced without significant changes in function. Seven replacements, all conservative, occur in the interior of the protein; the remainder are on the surface. All residues known to be components of the active site of bovine trypsin are present in corresponding positions in dogfish trypsin. Comparison of the three enzymes suggests calcium binding sites in dogfish trypsin. A corrected sequence of bovine trypsin identifies residue 67 as Asn and residues 84-87 as Ser-Asn-Thr-Leu."}
{"id": "PMID:1092333", "title": "The nature of protein association with chromatin.", "content": "The reality of the nonrandom distribution of histones along the chromatin strand was investigated in several ways. It does not appear to derive from histone exchange during shearing and is evident in chromatin fixed with formaldehyde prior to shearing. Endogenous or Escherichia coli polymerase are preferentially associated with regions of chromatin with a low protein/DNAratio. Although RNA polymerase and histones are fixed to chromatin after formaldehyde treatment with high efficiency, only a minor fraction of non-histone protein is fixed under similar conditions. Even after washing in high salt to minimize adventitious association, most remaining non-histone protein fails to be fixed. The utility of this approach for defining chromosomal proteins is discussed.", "contents": "The nature of protein association with chromatin. The reality of the nonrandom distribution of histones along the chromatin strand was investigated in several ways. It does not appear to derive from histone exchange during shearing and is evident in chromatin fixed with formaldehyde prior to shearing. Endogenous or Escherichia coli polymerase are preferentially associated with regions of chromatin with a low protein/DNAratio. Although RNA polymerase and histones are fixed to chromatin after formaldehyde treatment with high efficiency, only a minor fraction of non-histone protein is fixed under similar conditions. Even after washing in high salt to minimize adventitious association, most remaining non-histone protein fails to be fixed. The utility of this approach for defining chromosomal proteins is discussed."}
{"id": "PMID:1092334", "title": "Comparative ability of RNA and DNA to prime DNA synthesis in vitro: role of sequence, sugar, and structure of template-primer.", "content": "The priming efficiency of oligo(RNA) vs. oligo(DNA) in a homopolymer template-homooligomer primer system was compared using four DNA polymerases. The templates included (dT)n, (dA)n, (dC)n, and (dI)n. Primers were the oligomers of the complementary DNA OR RNA with chain lengths of 6 to 23. The DNA polymerases used were from Micrococcus luteus, avian myeloblastosis virus (AMV), and Escherichia coli (polymerase I and polymerase III). The polymerases demonstrated a preference for the DNA primers with (dC)n, (dA)n, and (dI)n templates. However, when (dT)n was the template, all but the AMV polymerase preferred (rA)11 more than 200-fold better than (dA)12. This preference was due to the physical structure of the initiation complex. The structures of the oligo-polymer complexes were characterized by mixing curves, melting curves, and analytical bouyant density analyses. (rA)11 + (dT)n formed predominatly a duplex structure, whereas (dA)12 + (dT)n formed the three- stranded structure, (dA12-2(dT)n. The Km of the duplex with E. coli Pol III was 2.9 mugM (rA)11. The Ki of the triplex was 2.2 mugM (dA)12, indicating that Pol III could bind to the triplex but would not elongate the (dA)12 primer. The influence of structure on priming also was demonstrated with longer oligomers, (dA)23 and (rA)23, where the (dA)23 formed more duplex-like structures and primed more than the (dA)12.(dT)10 + (dA)n complexes also were shown to form triplex structures that inhibited priming. These results show that template-primer structure has more influence on priming than the sugar moiety or the sequence of the nucleic acid.", "contents": "Comparative ability of RNA and DNA to prime DNA synthesis in vitro: role of sequence, sugar, and structure of template-primer. The priming efficiency of oligo(RNA) vs. oligo(DNA) in a homopolymer template-homooligomer primer system was compared using four DNA polymerases. The templates included (dT)n, (dA)n, (dC)n, and (dI)n. Primers were the oligomers of the complementary DNA OR RNA with chain lengths of 6 to 23. The DNA polymerases used were from Micrococcus luteus, avian myeloblastosis virus (AMV), and Escherichia coli (polymerase I and polymerase III). The polymerases demonstrated a preference for the DNA primers with (dC)n, (dA)n, and (dI)n templates. However, when (dT)n was the template, all but the AMV polymerase preferred (rA)11 more than 200-fold better than (dA)12. This preference was due to the physical structure of the initiation complex. The structures of the oligo-polymer complexes were characterized by mixing curves, melting curves, and analytical bouyant density analyses. (rA)11 + (dT)n formed predominatly a duplex structure, whereas (dA)12 + (dT)n formed the three- stranded structure, (dA12-2(dT)n. The Km of the duplex with E. coli Pol III was 2.9 mugM (rA)11. The Ki of the triplex was 2.2 mugM (dA)12, indicating that Pol III could bind to the triplex but would not elongate the (dA)12 primer. The influence of structure on priming also was demonstrated with longer oligomers, (dA)23 and (rA)23, where the (dA)23 formed more duplex-like structures and primed more than the (dA)12.(dT)10 + (dA)n complexes also were shown to form triplex structures that inhibited priming. These results show that template-primer structure has more influence on priming than the sugar moiety or the sequence of the nucleic acid."}
{"id": "PMID:1092335", "title": "The effect of modification of T7 DNA by the carcinogen N-1-acetylaminofluorene: termination of transcription in vitro.", "content": "To study the effects of N-2-acetylaminofluorene (AAF) modification of DNA on transcription, purified DNA from bacteriophage T7 was modified in vitro to varying extent with AAF and transcribed by DNA-dependent RNA polymerase from Escherichia coli. The main effects of AAF modification on transcription are a marked inhibition of the rate and extent of trna synthesis with relatively little effect on initiation except at very high AAF doses. Calibration of the percent modification with [14-C]AAF and analysis of the size of the RNA product by double isotope labeling and polyacrylamide gel electrophoresis support the following mechanism of transcription inhibition: most of the AAF residues bound to the coding strand of the DNA cause premature termination of transcription, at or near the site of modification, with release of RNA polymerase. This results in the production of shorter RNA chains with increasing amounts of bound carcinogen. The data are consistent with there being no reinitiation and/or synthesis of RNA distal to the AAF-modification site.", "contents": "The effect of modification of T7 DNA by the carcinogen N-1-acetylaminofluorene: termination of transcription in vitro. To study the effects of N-2-acetylaminofluorene (AAF) modification of DNA on transcription, purified DNA from bacteriophage T7 was modified in vitro to varying extent with AAF and transcribed by DNA-dependent RNA polymerase from Escherichia coli. The main effects of AAF modification on transcription are a marked inhibition of the rate and extent of trna synthesis with relatively little effect on initiation except at very high AAF doses. Calibration of the percent modification with [14-C]AAF and analysis of the size of the RNA product by double isotope labeling and polyacrylamide gel electrophoresis support the following mechanism of transcription inhibition: most of the AAF residues bound to the coding strand of the DNA cause premature termination of transcription, at or near the site of modification, with release of RNA polymerase. This results in the production of shorter RNA chains with increasing amounts of bound carcinogen. The data are consistent with there being no reinitiation and/or synthesis of RNA distal to the AAF-modification site."}
{"id": "PMID:1092336", "title": "Europium as a fluorescent probe of transfer RNA structure.", "content": "The binding of europium(III) to Escherichia coli tRNA-fMet,Glu and to unfractionated E. coli tRNA has been investigated by using the 4-thiouridine sensitization of europium 5-Do yields 7-F1 emission and changes in the lifetime of the 5-Do state of europium reported earlier (J. M. Wolfson and D. R. Kearns (1974), J. Am. Chem. Soc. 96, 3653). Binding of the first 3-4 europium ions is independent and sequential, approximately 600 times stronger than the magnesium binding, and the binding sites are located near the 4-thiouridine residue found at position 8 in a number of E. coli tRNA. Competition experiments suggest the strong binding sites are the same for magnesium and europium. The europium binding properties of both unfractionated E. coli tRNA and purified tRNA-fMet are quite similar, indicating that the location of the strong binding sites and their binding constants are nearly the same for a large group of tRNA. The europium binding properties of native and denatured tRNA are quite different, however.", "contents": "Europium as a fluorescent probe of transfer RNA structure. The binding of europium(III) to Escherichia coli tRNA-fMet,Glu and to unfractionated E. coli tRNA has been investigated by using the 4-thiouridine sensitization of europium 5-Do yields 7-F1 emission and changes in the lifetime of the 5-Do state of europium reported earlier (J. M. Wolfson and D. R. Kearns (1974), J. Am. Chem. Soc. 96, 3653). Binding of the first 3-4 europium ions is independent and sequential, approximately 600 times stronger than the magnesium binding, and the binding sites are located near the 4-thiouridine residue found at position 8 in a number of E. coli tRNA. Competition experiments suggest the strong binding sites are the same for magnesium and europium. The europium binding properties of both unfractionated E. coli tRNA and purified tRNA-fMet are quite similar, indicating that the location of the strong binding sites and their binding constants are nearly the same for a large group of tRNA. The europium binding properties of native and denatured tRNA are quite different, however."}
{"id": "PMID:1092337", "title": "Kinetic studies on coenzyme binding and coenzyme dissociation in tryptophanase immobilized on sepharose.", "content": "The binding rate of pyridoxal '5-phosphate (Pxa-P) to apotryptophanase and the dissociation rate of the coenzyme from holotryptophanase were able to be determined by following the enzyme activity in continuous flow reactions on a column of immobilized tryptophanase. When the enzyme activity was assayed continuously in the flow system in the absence of coenzyme added to the reaction mixture, immobilized holotryptophanase lost gradually its initial activity owing to dissociation of coenzyme. The coenzyme dissociation at a given concentration of substrate (tryptophan) followed first-order kineticsmin a low substrate concentration range below the Km value, a more decreased rate constant was obtained for the coenzyme dissociation. This indicates that the coenzyme is more dissociable from the apoenzyme-coenzyme-substrate complex (ECS complex) rather than from the apoenzyme-coenzymecomplex (holoenzyme). Immobilized tryptophanase freed of coenzyme restored rapidly its original activity, when the assay mixture containing a given concentration of substrate and Pxa-P was passed through the immobilized enzyme column. The coenzyme binding at a given coenzyme concentration followed first-order kinetics, but the rate was not first order in regard to the coenzyme concentration. A plot of the reciprocal of the first-order rate constant obtained vs. the reciprocal of the coenzyme binding occurs in a two-step fashion; the first step is rapid and the second step is rate determining. Both the dissociation constant for the first step and the rate constant for the second step were shown to be independent of the substrate concentration. This means that Schiff base formation between Pxa-P and tryptophan in the assay mixture has no effect on the binding of Pxa-P to apoenzyme. The coenzyme dissociation constant at a given substrate concentration was calculated from both the rate constant of the coenzyme binding and the rate constant of the coenzyme dissociation. The values obtained by this method at different substrate concentrations were almost identical with those measured at the corresponding substrate concentrations directly by an ordinary method.", "contents": "Kinetic studies on coenzyme binding and coenzyme dissociation in tryptophanase immobilized on sepharose. The binding rate of pyridoxal '5-phosphate (Pxa-P) to apotryptophanase and the dissociation rate of the coenzyme from holotryptophanase were able to be determined by following the enzyme activity in continuous flow reactions on a column of immobilized tryptophanase. When the enzyme activity was assayed continuously in the flow system in the absence of coenzyme added to the reaction mixture, immobilized holotryptophanase lost gradually its initial activity owing to dissociation of coenzyme. The coenzyme dissociation at a given concentration of substrate (tryptophan) followed first-order kineticsmin a low substrate concentration range below the Km value, a more decreased rate constant was obtained for the coenzyme dissociation. This indicates that the coenzyme is more dissociable from the apoenzyme-coenzyme-substrate complex (ECS complex) rather than from the apoenzyme-coenzymecomplex (holoenzyme). Immobilized tryptophanase freed of coenzyme restored rapidly its original activity, when the assay mixture containing a given concentration of substrate and Pxa-P was passed through the immobilized enzyme column. The coenzyme binding at a given coenzyme concentration followed first-order kinetics, but the rate was not first order in regard to the coenzyme concentration. A plot of the reciprocal of the first-order rate constant obtained vs. the reciprocal of the coenzyme binding occurs in a two-step fashion; the first step is rapid and the second step is rate determining. Both the dissociation constant for the first step and the rate constant for the second step were shown to be independent of the substrate concentration. This means that Schiff base formation between Pxa-P and tryptophan in the assay mixture has no effect on the binding of Pxa-P to apoenzyme. The coenzyme dissociation constant at a given substrate concentration was calculated from both the rate constant of the coenzyme binding and the rate constant of the coenzyme dissociation. The values obtained by this method at different substrate concentrations were almost identical with those measured at the corresponding substrate concentrations directly by an ordinary method."}
{"id": "PMID:1092338", "title": "Isolation and partial characterization of anglefish proglucagon.", "content": "Evidence is presented that proglucagon from anglefish islets is a single chain polypeptide with 78 amino acid residues and that the glucagon portion of it is liberated after tryptic cleavage. The most striking characteristic in the conversion of the anglerfish proglucagon to glucagon is that the cleaved peptide bonds display enormous sensitivity toward trypsin. Thus, conversion of the prohormone to glucagon occurs very rapidly within 3-10 min with a 1:500-1:1000 molar ratio of enzyme to substrate. Further, trypic cleavage of the anglerfish glucagon requires higher concentrations of trypsin (molar ratio 1:25 enzyme to substrate) and longer incubation time. The behavior of proglucagon and glucagon toward trypsin shows striking similarities with the tryptic conversion of anglerfish proinsulin to insulin.", "contents": "Isolation and partial characterization of anglefish proglucagon. Evidence is presented that proglucagon from anglefish islets is a single chain polypeptide with 78 amino acid residues and that the glucagon portion of it is liberated after tryptic cleavage. The most striking characteristic in the conversion of the anglerfish proglucagon to glucagon is that the cleaved peptide bonds display enormous sensitivity toward trypsin. Thus, conversion of the prohormone to glucagon occurs very rapidly within 3-10 min with a 1:500-1:1000 molar ratio of enzyme to substrate. Further, trypic cleavage of the anglerfish glucagon requires higher concentrations of trypsin (molar ratio 1:25 enzyme to substrate) and longer incubation time. The behavior of proglucagon and glucagon toward trypsin shows striking similarities with the tryptic conversion of anglerfish proinsulin to insulin."}
{"id": "PMID:1092339", "title": "Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations.", "content": "The Mg2+-dependent equilibrium of ribosomal subunits was studied by light scattering in the absence of any other factor of protein synthesis. The difference of intensity in the light scattered by dissociated 30S-50S couples and by 70S particles was used as a method to measure the percentage of association as a function of Mg2+ under conditions of nonperturbation of the equilibrium. We found that pressure-resistant (type A) and non-pressure-resistant (type B) ribosomes may be characterized by the different behavior of their association equilibrium curves. The thermodynamic parameters of this equilibrium and their comparison for the two classes of ribosomes were calculated from experiments at different temperatures.", "contents": "Ribosomal subunit interaction as studied by light scattering. Evidence of different classes of ribosome preparations. The Mg2+-dependent equilibrium of ribosomal subunits was studied by light scattering in the absence of any other factor of protein synthesis. The difference of intensity in the light scattered by dissociated 30S-50S couples and by 70S particles was used as a method to measure the percentage of association as a function of Mg2+ under conditions of nonperturbation of the equilibrium. We found that pressure-resistant (type A) and non-pressure-resistant (type B) ribosomes may be characterized by the different behavior of their association equilibrium curves. The thermodynamic parameters of this equilibrium and their comparison for the two classes of ribosomes were calculated from experiments at different temperatures."}
{"id": "PMID:1092340", "title": "Identification of the collagenous proteins synthesized by cultured cells from human skin.", "content": "Cells cultured from human skin synthesize precursor forms of types I and III collagens. After denaturation and reduction, the polypeptide chains obtained from these molecules had molecular weights estimated to be 140,000 and 120, 000. The larger chains, the pro alpha chains, are believed to be derived from the original precursor molecules. The smaller chains arise from altered forms, p-collagens, that may be normal intermediates in the conversion of both procollagens to collagens I and III.", "contents": "Identification of the collagenous proteins synthesized by cultured cells from human skin. Cells cultured from human skin synthesize precursor forms of types I and III collagens. After denaturation and reduction, the polypeptide chains obtained from these molecules had molecular weights estimated to be 140,000 and 120, 000. The larger chains, the pro alpha chains, are believed to be derived from the original precursor molecules. The smaller chains arise from altered forms, p-collagens, that may be normal intermediates in the conversion of both procollagens to collagens I and III."}
{"id": "PMID:1092341", "title": "Some characteristics of and structural requirements for the interaction of 24,25-dihydrofusidic acid with ribosome - elongation factor g Complexes.", "content": "Fusidic acid inhibits polypeptide chain elongation by binding to the ribosome - elongation factor-G - GDP complex and thereby preventing its dissociation. The experiments reported here quantitate the interaction of the antibiotic [3H]-24,25-dihydrofusidic acid, an active analog of fusidic acid, with the ribosome - elongation factor-G - GDP comples. All components of the complex are essential for [3H]-24,25-dihydrofusidic acid binding. The stoichiometry of the interaction is ca. 1:1, and the Ka apparent, as determined by equilibrium dialysis, is 2.6 times 10-6 M-minus 1. It is further shown that GTP and GDP are equally effective in forming complexes to which the antibiotic may bind, whereas GMP and beta,gamma-methyleneguanosine triphosphate will not form complexes to which the antibiotic may bind. In order to examine the structural basis of the mode of antibiotic action shown by fusidic acid, we have considered two activities of 21 structural analogs of this antibiotic: ability to bind to the aforementioned ternary complex and ability to stabilize this complex. The comparative binding capability of the analogs were extablished through competition experiments with [3H]-24,25-dihydrofusidic acid. The data obtained from these experiments can be summarized as follows. (1) The C17-20 double bond of fusidic acid appears to be critical for both binding and complex stabilization activities. (2) A carboxyl group in the vicinity of the C20 carbon is also essential for both activities. (3) Modifications of other functional groups in the molecule can lead to significantly decreased stabilization of the ternary ribosome complex and/or ability to compete with [3H]-24,25-dihydrofusidic acid for binding to the complex, but do not demonstrate absolute structural requirements for either activity.", "contents": "Some characteristics of and structural requirements for the interaction of 24,25-dihydrofusidic acid with ribosome - elongation factor g Complexes. Fusidic acid inhibits polypeptide chain elongation by binding to the ribosome - elongation factor-G - GDP complex and thereby preventing its dissociation. The experiments reported here quantitate the interaction of the antibiotic [3H]-24,25-dihydrofusidic acid, an active analog of fusidic acid, with the ribosome - elongation factor-G - GDP comples. All components of the complex are essential for [3H]-24,25-dihydrofusidic acid binding. The stoichiometry of the interaction is ca. 1:1, and the Ka apparent, as determined by equilibrium dialysis, is 2.6 times 10-6 M-minus 1. It is further shown that GTP and GDP are equally effective in forming complexes to which the antibiotic may bind, whereas GMP and beta,gamma-methyleneguanosine triphosphate will not form complexes to which the antibiotic may bind. In order to examine the structural basis of the mode of antibiotic action shown by fusidic acid, we have considered two activities of 21 structural analogs of this antibiotic: ability to bind to the aforementioned ternary complex and ability to stabilize this complex. The comparative binding capability of the analogs were extablished through competition experiments with [3H]-24,25-dihydrofusidic acid. The data obtained from these experiments can be summarized as follows. (1) The C17-20 double bond of fusidic acid appears to be critical for both binding and complex stabilization activities. (2) A carboxyl group in the vicinity of the C20 carbon is also essential for both activities. (3) Modifications of other functional groups in the molecule can lead to significantly decreased stabilization of the ternary ribosome complex and/or ability to compete with [3H]-24,25-dihydrofusidic acid for binding to the complex, but do not demonstrate absolute structural requirements for either activity."}
{"id": "PMID:1092342", "title": "A comparative study of the 50S ribosomal subunit and several 50S subparticles in EF-T-and EF-G-dependent activities.", "content": "A series of ribosomal subparticles derived from the 50S subunit has been studied and compared in EF-T- and EF-G-dependent reactions. Three different 50S cores were prepared by CsC1 isophycnic centrifugation and one by NH(4)Cl-ethanol extractionm the 50S CsCl core a had lost proteins L1, L7, L8, L10, L12, L16, L25, L33, and some L6 and L11. The 50S CsCl core b additionally lacked protein L6, and 50S CsCl core c also lacked protein L5, L15, L18, L27, L28, L30, and most of L9, L14, L19, and L21. The 50S NH(4)Cl-ethanol core had lost up to 90 percent of proteins L7, L12 and 30-60 percent of proteins L8, L10, and L29. The 50S CsCl core a had much reduced activity in EF-G and none in EF-T GTPase reactions while 50S CsCl cores b and c were inactive. Addition of proteins L7, L12 restored the activity for both the EF-T- and EF-G-dependent GTPase with all of the three 50S CsCl cores, increasing stepwise from core c to core a; The 50S NH(4)Cl-ethanol core was partially active in the EF-G GTPase over the 2-30 mM MG-2+ range tested, while EF-T only showed some activity inthe upper portion of this range...", "contents": "A comparative study of the 50S ribosomal subunit and several 50S subparticles in EF-T-and EF-G-dependent activities. A series of ribosomal subparticles derived from the 50S subunit has been studied and compared in EF-T- and EF-G-dependent reactions. Three different 50S cores were prepared by CsC1 isophycnic centrifugation and one by NH(4)Cl-ethanol extractionm the 50S CsCl core a had lost proteins L1, L7, L8, L10, L12, L16, L25, L33, and some L6 and L11. The 50S CsCl core b additionally lacked protein L6, and 50S CsCl core c also lacked protein L5, L15, L18, L27, L28, L30, and most of L9, L14, L19, and L21. The 50S NH(4)Cl-ethanol core had lost up to 90 percent of proteins L7, L12 and 30-60 percent of proteins L8, L10, and L29. The 50S CsCl core a had much reduced activity in EF-G and none in EF-T GTPase reactions while 50S CsCl cores b and c were inactive. Addition of proteins L7, L12 restored the activity for both the EF-T- and EF-G-dependent GTPase with all of the three 50S CsCl cores, increasing stepwise from core c to core a; The 50S NH(4)Cl-ethanol core was partially active in the EF-G GTPase over the 2-30 mM MG-2+ range tested, while EF-T only showed some activity inthe upper portion of this range..."}
{"id": "PMID:1092343", "title": "Polyfluoroalkylamine derivatives of luteinizing hormone-releasing hormone.", "content": "A series of C-terminally fluorinated analogs of luteinizing hormone-releasing hormone (LH-RH) was synthesized by a combination of solid-phase and classical techniques and found to have interesting properties when assayed for LH- and FSH-releasing activities over a prolonged period of time in immature male rats. One of the peptides, desGly-10-LH-RH-2,2,2-trifluoroethylamide, was found to be about nine times more effective than LH-RH in releasing LH, or approximately twice as active as the corresponding alkylamide peptide, desGly-10-LH-RH-ethylamide. However, desGly-10-LH-RH-2,2,3,3,3-pentafluoropropylamide was only slightly more active than LH-RH and considerably less active than the corresponding propylamide analog. A family of peptides was also prepared containing D-alanine in position six of the chain in conjunction with the C-terminal modifications. D-Ala-6,desGly-10-LH-RH-2,2,2-trifluoroethylamide, surprisingly, gave patterns of gonadotropin release which were only as intense and virtually identical with those obtained with D-Ala-6,desGly-10-LH-RH-ethylamide which in turn releases about 20 times more LH than a similar dose of LH-RH. D-Ala-6,desGly-10-LH-RH-propylamide also gave almost identical patterns of gonadotropin release. D-Ala-6,desGly-10,LH-RH-2,2,3,3,3-pentafluoropropylamide was considerably less potent, being only five times more effective than LH-RH.", "contents": "Polyfluoroalkylamine derivatives of luteinizing hormone-releasing hormone. A series of C-terminally fluorinated analogs of luteinizing hormone-releasing hormone (LH-RH) was synthesized by a combination of solid-phase and classical techniques and found to have interesting properties when assayed for LH- and FSH-releasing activities over a prolonged period of time in immature male rats. One of the peptides, desGly-10-LH-RH-2,2,2-trifluoroethylamide, was found to be about nine times more effective than LH-RH in releasing LH, or approximately twice as active as the corresponding alkylamide peptide, desGly-10-LH-RH-ethylamide. However, desGly-10-LH-RH-2,2,3,3,3-pentafluoropropylamide was only slightly more active than LH-RH and considerably less active than the corresponding propylamide analog. A family of peptides was also prepared containing D-alanine in position six of the chain in conjunction with the C-terminal modifications. D-Ala-6,desGly-10-LH-RH-2,2,2-trifluoroethylamide, surprisingly, gave patterns of gonadotropin release which were only as intense and virtually identical with those obtained with D-Ala-6,desGly-10-LH-RH-ethylamide which in turn releases about 20 times more LH than a similar dose of LH-RH. D-Ala-6,desGly-10-LH-RH-propylamide also gave almost identical patterns of gonadotropin release. D-Ala-6,desGly-10,LH-RH-2,2,3,3,3-pentafluoropropylamide was considerably less potent, being only five times more effective than LH-RH."}
{"id": "PMID:1092344", "title": "Evidence for fidelity of chromatin reconstitution.", "content": "Several lines of evidence are presented which support the contention that chromatin may be dissociated, fractionated, and reconstituted without altering the compositional, structural, or transcriptional integrity of the genome. The similar compositions of native and reconstituted chromatins are suggested by the absence of significant differences in their protein/DNA ratios and in the polyacrylamide gel electrophoretic profiles of their histones and nonhistone chromosomal proteins. Criteria for fidelity of genome structure in reconstituted chromatin include binding of reporter molecules with specificity for the minor groove of DNA, binding of histones, number of sites available for addition of nucleotides, and circular dichroism spectra. When the transcriptional activities of native and reconstituted chromatins were compared under conditions where reinitiation is prohibited, significant changes were not observed. Taken together, the present results strongly suggest, but do not conclusively establish, fidelity of chromatin reconstitution.", "contents": "Evidence for fidelity of chromatin reconstitution. Several lines of evidence are presented which support the contention that chromatin may be dissociated, fractionated, and reconstituted without altering the compositional, structural, or transcriptional integrity of the genome. The similar compositions of native and reconstituted chromatins are suggested by the absence of significant differences in their protein/DNA ratios and in the polyacrylamide gel electrophoretic profiles of their histones and nonhistone chromosomal proteins. Criteria for fidelity of genome structure in reconstituted chromatin include binding of reporter molecules with specificity for the minor groove of DNA, binding of histones, number of sites available for addition of nucleotides, and circular dichroism spectra. When the transcriptional activities of native and reconstituted chromatins were compared under conditions where reinitiation is prohibited, significant changes were not observed. Taken together, the present results strongly suggest, but do not conclusively establish, fidelity of chromatin reconstitution."}
{"id": "PMID:1092345", "title": "Conformations and structural transitions in polydeoxynucleotides.", "content": "Polydeoxynucleotides of different base sequence, the alternating poly[d(A-T)]-poly[d(A-T)], crab satellite DNA, on the one hand, and double-stranded homopolymer complexes poly[d(A)]-poly[d(T)], poly[d(I)]-poly[d(C)], on the other, display significant differences in their conformation and conformational transitions. Infrared linear dichroism investigations indicate that the alternating poly[d(A-T)]-poly[d(A-T)], enzymatically synthesized, adopts a lower humidity a well-expressed A* form in which stability is relatively small,i.e., restricted to limited relative humidity. This A form is characterized by the orientation of the bisector of the phosphate OPO group at 34 degrees with respect to the helical axis, which is slightly lower than that of DNA. In contrast, for the homopolynucleotide double-stranded complex poly(dA)-poly(dT) and also for poly(dI)-poly(dC), the B yields A conformational change is not observed. Instead poly(dA)-poly(dT) exists at lower humidity in a stable modified B form. Thus the present results indicate that homo(dA)-homo(dT) double-stranded sequences prevent the B yields A structural transition. All AT-containing polydeoxynucleotides and crab satellite DNA adopt a high humidity a modified B form characterized by the orientation of the bisector of the phosphate group OPO at 64 degrees with respect to the helical axis which is significantly lower than 68-74 degrees observed in DNAs. The base pairing geometry in poly(dA)-poly(dT), poly[d(A-T)]-poly[d(A-T)], and also in poly(dI)-poly(dC) is apparently a Watson and Crick type. Thus the observed differences in conformation are not due to different base pairing scheme. It is suggested that in DNAs of high AT content the presence of homo(dT)-homo(dA) sequences and the relatively low stability of the A form of d(A-T) alternating sequences may inhibit the change to the A form. A possible role of these sequences in DNA recognition by protein is suggested.", "contents": "Conformations and structural transitions in polydeoxynucleotides. Polydeoxynucleotides of different base sequence, the alternating poly[d(A-T)]-poly[d(A-T)], crab satellite DNA, on the one hand, and double-stranded homopolymer complexes poly[d(A)]-poly[d(T)], poly[d(I)]-poly[d(C)], on the other, display significant differences in their conformation and conformational transitions. Infrared linear dichroism investigations indicate that the alternating poly[d(A-T)]-poly[d(A-T)], enzymatically synthesized, adopts a lower humidity a well-expressed A* form in which stability is relatively small,i.e., restricted to limited relative humidity. This A form is characterized by the orientation of the bisector of the phosphate OPO group at 34 degrees with respect to the helical axis, which is slightly lower than that of DNA. In contrast, for the homopolynucleotide double-stranded complex poly(dA)-poly(dT) and also for poly(dI)-poly(dC), the B yields A conformational change is not observed. Instead poly(dA)-poly(dT) exists at lower humidity in a stable modified B form. Thus the present results indicate that homo(dA)-homo(dT) double-stranded sequences prevent the B yields A structural transition. All AT-containing polydeoxynucleotides and crab satellite DNA adopt a high humidity a modified B form characterized by the orientation of the bisector of the phosphate group OPO at 64 degrees with respect to the helical axis which is significantly lower than 68-74 degrees observed in DNAs. The base pairing geometry in poly(dA)-poly(dT), poly[d(A-T)]-poly[d(A-T)], and also in poly(dI)-poly(dC) is apparently a Watson and Crick type. Thus the observed differences in conformation are not due to different base pairing scheme. It is suggested that in DNAs of high AT content the presence of homo(dT)-homo(dA) sequences and the relatively low stability of the A form of d(A-T) alternating sequences may inhibit the change to the A form. A possible role of these sequences in DNA recognition by protein is suggested."}
{"id": "PMID:1092346", "title": "Amino acid sequence of two functional sites in yeast glycogen phosphorylase.", "content": "The structure of two functional sites in baker's yeast (Saccharomyces cerevisiae) glycogen phosphorylase (EC 2.4 1.1) was determined as part of a study on the evolution of regulatory enzymes. S-Carboxymethylated, MaBH4-reduced 32-P-labeled yeast phosphorylase a was cleaved with CNBr, thermolysin, and pepsin. Peptides labeled with 32-P or carrying the fluorescent pyridoxyl marker were isolated and purified using ion-exchange chromatography and gel filtration. CNBr cleavage yielded a single radioactive phosphopeptide (42 residues long) and one small fluorescent peptide with the unique sequence epsilon-Pxy-Lys-Phe-Val-Met. Thermolysin digestion gave rise to one radioactive octapeptide and two fluorescent peptides, 15 and 2 residues long, respectively. From a combination of substractive Edman degradations and digestion with yeast protease C, the sequence of the 32-P-labeled octapeptide was established. Phosphothreonine was identified as the sole phosphorylated amino acid, giving the following structure for the site involved in the covalent regulation of yeast phosphorylase: Leu-Thr(P) -Gly-Phe-Leu-Pro-Gln-Glu. The two fluorescent thermolytic peptides, together with two additional pyridoxyl peptides isolated after peptic digestion of the enzyme yielded the following sequence around the site binding pyridoxal-5'-P, the cofactor essential for phosphorylase activity: Ile-Ser-Thr-Ala-Gly-Thr-Glu-Ala-Ser-Gly-Thr-Ser-Asn-Met-Lys(P Pxy)-Phe-Val-Met. While the phosphorylated site bears no resemblance to the site of covalent control in vertebrate phosphorylases, the pyridoxal-P binding site in the yeast enayme displays remarkable homologies with its animal counterparts; the finding that 14 out of 18 amino acids are identical strongly suggests that the cofactor must be directly involved in catalysis.", "contents": "Amino acid sequence of two functional sites in yeast glycogen phosphorylase. The structure of two functional sites in baker's yeast (Saccharomyces cerevisiae) glycogen phosphorylase (EC 2.4 1.1) was determined as part of a study on the evolution of regulatory enzymes. S-Carboxymethylated, MaBH4-reduced 32-P-labeled yeast phosphorylase a was cleaved with CNBr, thermolysin, and pepsin. Peptides labeled with 32-P or carrying the fluorescent pyridoxyl marker were isolated and purified using ion-exchange chromatography and gel filtration. CNBr cleavage yielded a single radioactive phosphopeptide (42 residues long) and one small fluorescent peptide with the unique sequence epsilon-Pxy-Lys-Phe-Val-Met. Thermolysin digestion gave rise to one radioactive octapeptide and two fluorescent peptides, 15 and 2 residues long, respectively. From a combination of substractive Edman degradations and digestion with yeast protease C, the sequence of the 32-P-labeled octapeptide was established. Phosphothreonine was identified as the sole phosphorylated amino acid, giving the following structure for the site involved in the covalent regulation of yeast phosphorylase: Leu-Thr(P) -Gly-Phe-Leu-Pro-Gln-Glu. The two fluorescent thermolytic peptides, together with two additional pyridoxyl peptides isolated after peptic digestion of the enzyme yielded the following sequence around the site binding pyridoxal-5'-P, the cofactor essential for phosphorylase activity: Ile-Ser-Thr-Ala-Gly-Thr-Glu-Ala-Ser-Gly-Thr-Ser-Asn-Met-Lys(P Pxy)-Phe-Val-Met. While the phosphorylated site bears no resemblance to the site of covalent control in vertebrate phosphorylases, the pyridoxal-P binding site in the yeast enayme displays remarkable homologies with its animal counterparts; the finding that 14 out of 18 amino acids are identical strongly suggests that the cofactor must be directly involved in catalysis."}
{"id": "PMID:1092347", "title": "Red cell surface structure. Stabilization by cholesterol sulfate as evidenced by scanning electron microscopy.", "content": "Scanning electron microscopic studies demonstrate that the normal biconcave shape of the human erythrocyte is maintained in hypotonic saline when physiological levels (10 minus 5M) of cholesterol sulfate are added to the solutions. Cholesterol sulfate is a naturally occurring sterol conjugate in plasma and erythrocyte membranes and we propose that it may belong to that class of amphipathic molecules responsible for the maintenance of structure of the erythrocyte by interaction with membrane components.", "contents": "Red cell surface structure. Stabilization by cholesterol sulfate as evidenced by scanning electron microscopy. Scanning electron microscopic studies demonstrate that the normal biconcave shape of the human erythrocyte is maintained in hypotonic saline when physiological levels (10 minus 5M) of cholesterol sulfate are added to the solutions. Cholesterol sulfate is a naturally occurring sterol conjugate in plasma and erythrocyte membranes and we propose that it may belong to that class of amphipathic molecules responsible for the maintenance of structure of the erythrocyte by interaction with membrane components."}
{"id": "PMID:1092348", "title": "Redox properties of beta-type cytochromes in Escherichia coli and rat liver mitochondria and techniques for their analysis.", "content": "We describe here apparatus and procedures for conducting potentiometric titrations and for analyzing the collected data in terms of the number of components present, their amounts and their midpoint potentials. Using these procedures we have determined the presence of three forms of cytochrome b1 in Escherichia coli with midpoint potentials at pH 7.1 OF about minus 50, plus 110 and plus 220 mV. We were not able to demonstrate a change in any of these potentials by the addition of phosphate, ATP, or 2, 4-dinitrophenol. We have been able to confirm the presence of two forms of cytochrome b in non-energized mitochondria and the apparent conversion of the low-potential component to a new high potential component upon energization of the mitochondria. However we cite further experimental data that question the actual conversion of one form of cytochrome b to another. An alternative interpretation based on our analysis suggests that the high voltage component may be present in a masked form in the non-energized mitochondria.", "contents": "Redox properties of beta-type cytochromes in Escherichia coli and rat liver mitochondria and techniques for their analysis. We describe here apparatus and procedures for conducting potentiometric titrations and for analyzing the collected data in terms of the number of components present, their amounts and their midpoint potentials. Using these procedures we have determined the presence of three forms of cytochrome b1 in Escherichia coli with midpoint potentials at pH 7.1 OF about minus 50, plus 110 and plus 220 mV. We were not able to demonstrate a change in any of these potentials by the addition of phosphate, ATP, or 2, 4-dinitrophenol. We have been able to confirm the presence of two forms of cytochrome b in non-energized mitochondria and the apparent conversion of the low-potential component to a new high potential component upon energization of the mitochondria. However we cite further experimental data that question the actual conversion of one form of cytochrome b to another. An alternative interpretation based on our analysis suggests that the high voltage component may be present in a masked form in the non-energized mitochondria."}
{"id": "PMID:1092349", "title": "Sequence homology of nuclear and mitochondrial DNAs of different yeasts.", "content": "1. Both nuclear and mtDNA of four different yeasts show approximately 10% homology as measured by DNA-DNA filter hybridization. These homologous sequences are mainly attributable to the ribosomal cistrons. 2. Melting curve analysis shows that the heterologous mitochondrial DNA-DNA hybrids contain several times more mismatching than the nuclear DNA-DNA hybrids. 3. DNA-rRNA hybridization shows that the sequences of the ribosomal cistrons in both the nuclear and the mitochondrial genome have been conserved during evolution. 4. However, melting curve analysis of the DNA-RNA hybrids shows that the sequence of the nuclear ribosomal cistrons have undergone considerable fewer nucleotide substitutions than their mitochondrial counterparts. 5. The results suggest that the mitochondrial ribosomal cistrons have evolved more rapidly than the nuclear cistrons. This is discussed in the light of theories on the rat of molecular evolutin.", "contents": "Sequence homology of nuclear and mitochondrial DNAs of different yeasts. 1. Both nuclear and mtDNA of four different yeasts show approximately 10% homology as measured by DNA-DNA filter hybridization. These homologous sequences are mainly attributable to the ribosomal cistrons. 2. Melting curve analysis shows that the heterologous mitochondrial DNA-DNA hybrids contain several times more mismatching than the nuclear DNA-DNA hybrids. 3. DNA-rRNA hybridization shows that the sequences of the ribosomal cistrons in both the nuclear and the mitochondrial genome have been conserved during evolution. 4. However, melting curve analysis of the DNA-RNA hybrids shows that the sequence of the nuclear ribosomal cistrons have undergone considerable fewer nucleotide substitutions than their mitochondrial counterparts. 5. The results suggest that the mitochondrial ribosomal cistrons have evolved more rapidly than the nuclear cistrons. This is discussed in the light of theories on the rat of molecular evolutin."}
{"id": "PMID:1092350", "title": "Size, conformation and purity of chloroplast DNA of some higher plants.", "content": "1. Chloroplast DNA of Antirrhinum majus, Oenothera hookeri, Beta vulgaris and Spinacia oleracea band at the same buoyant density of 1.697 g-cm-3 in neutral CsCl equilibrium gradients. The corresponding nuclear DNAs band at 1.691, 1.703, 1.695 and 1.695 g-cm-3, respectively. The purity of chloroplast and nuclear DNA can be assessed objectively only in the cases of Antirrhinum and Oenothera. 2. Electron microscopic analysis of chloroplast DNA, purified in CsCl or CsCl/ethidium bromide gradients, revealed up to 80% circular molecules. Of these about 15% were of supertwisted conformation. Best yields of circular molecules were recovered when populations of unbroken chloroplasts were subjected to DNAase and phosphodiesterase treatment, and when the DNA was purified from viscous lysates by centrifugation into a CsCl cushion. Treatment of plastids with DNAase alone did not guarantee complete degradation of nuclear DNA. 3. The average contour length of the open circular chloroplast DNA molecules was basically similar for all four plants. They were 45.9 plus or minus 2.1 mum for Antirrhinum, 45.7 plus or minus 1.9 mum for Spinacia, 44.9 plus or minus 1.7 mum for Beta and 45.2 mum for Oenothera. This is comparable to the size derived for the coding capacity of chloroplast DNA from reassociation experiments. As much as 15% of the total population of circles in chloroplast DNA of Spinacia were circular dimers.", "contents": "Size, conformation and purity of chloroplast DNA of some higher plants. 1. Chloroplast DNA of Antirrhinum majus, Oenothera hookeri, Beta vulgaris and Spinacia oleracea band at the same buoyant density of 1.697 g-cm-3 in neutral CsCl equilibrium gradients. The corresponding nuclear DNAs band at 1.691, 1.703, 1.695 and 1.695 g-cm-3, respectively. The purity of chloroplast and nuclear DNA can be assessed objectively only in the cases of Antirrhinum and Oenothera. 2. Electron microscopic analysis of chloroplast DNA, purified in CsCl or CsCl/ethidium bromide gradients, revealed up to 80% circular molecules. Of these about 15% were of supertwisted conformation. Best yields of circular molecules were recovered when populations of unbroken chloroplasts were subjected to DNAase and phosphodiesterase treatment, and when the DNA was purified from viscous lysates by centrifugation into a CsCl cushion. Treatment of plastids with DNAase alone did not guarantee complete degradation of nuclear DNA. 3. The average contour length of the open circular chloroplast DNA molecules was basically similar for all four plants. They were 45.9 plus or minus 2.1 mum for Antirrhinum, 45.7 plus or minus 1.9 mum for Spinacia, 44.9 plus or minus 1.7 mum for Beta and 45.2 mum for Oenothera. This is comparable to the size derived for the coding capacity of chloroplast DNA from reassociation experiments. As much as 15% of the total population of circles in chloroplast DNA of Spinacia were circular dimers."}
{"id": "PMID:1092351", "title": "The 5'-termini of bacterial RNA. I. The monophosphorylated termini.", "content": "A two-dimensional fingerprint technique is described which separated oligonucleotides by length and number of phosphates present at the termini. The diversity of terminal nucleotide sequences was found to be quite restricted in bacterial 4-5 S RNA: in the strain used here the predominant dinucleotide species was pG-C, and the only trinucleotides produced were pG-G-Up and pG-G-Cp. Examination of total pulse-labeled RNA revealed the same restricted set of terminal trinucleotides.", "contents": "The 5'-termini of bacterial RNA. I. The monophosphorylated termini. A two-dimensional fingerprint technique is described which separated oligonucleotides by length and number of phosphates present at the termini. The diversity of terminal nucleotide sequences was found to be quite restricted in bacterial 4-5 S RNA: in the strain used here the predominant dinucleotide species was pG-C, and the only trinucleotides produced were pG-G-Up and pG-G-Cp. Examination of total pulse-labeled RNA revealed the same restricted set of terminal trinucleotides."}
{"id": "PMID:1092352", "title": "Simultaneous ribosomal resistance to trichodermin and anisomycin in Saccharomyces cerevisiae mutants.", "content": "A spontaneous mutant of Saccharomyces cerevisiae resistant to trichodermin has been isolated. It displays cross resistance both in vivo and in vitro to a number of sesquiterpene antibiotics (fusarenon X, trichothecin and verrucarin A) and to the chemically unrelated antibiotic anisomycin. The mutation conferring resistance to anisomycin and trichodermin is expressed in the 60-S subunit of the yeast 80-S ribosome. Mutant ribosomes bind [-14C]trichodermin much less efficiently than wild type ribosomes, suggesting that resistance may be due, at least in part, to this property. However, both types of ribosomes bind [-3H] anisomycin equally. These results suggest that anisomycin and trichodermin have different binding sites on the 60-S subunit of eukaryotic ribosomes, even though previous results have shown that both antibiotics bind to mutually exclusive sites.", "contents": "Simultaneous ribosomal resistance to trichodermin and anisomycin in Saccharomyces cerevisiae mutants. A spontaneous mutant of Saccharomyces cerevisiae resistant to trichodermin has been isolated. It displays cross resistance both in vivo and in vitro to a number of sesquiterpene antibiotics (fusarenon X, trichothecin and verrucarin A) and to the chemically unrelated antibiotic anisomycin. The mutation conferring resistance to anisomycin and trichodermin is expressed in the 60-S subunit of the yeast 80-S ribosome. Mutant ribosomes bind [-14C]trichodermin much less efficiently than wild type ribosomes, suggesting that resistance may be due, at least in part, to this property. However, both types of ribosomes bind [-3H] anisomycin equally. These results suggest that anisomycin and trichodermin have different binding sites on the 60-S subunit of eukaryotic ribosomes, even though previous results have shown that both antibiotics bind to mutually exclusive sites."}
{"id": "PMID:1092353", "title": "Ineffectiveness of rifampicin in inhibiting RNA synthesis in Escherichia coli and T(4)-infected Escherichia coli cells after exposure to ultraviolet radiation.", "content": "Resdiual RNA synthesis in Escherichia coli cells and T(4)-infected E. coli cells exposed to ultraviolet radiation is insensitive to rifampicin. On the other hand, residual RNA synthesis in these cells after gamma irradiation is further inhibited by rifampicin. The specific effect exerted by ultraviolet irradiation on transcription seems to arise from alterations in the DNA structure rather than in bacterial RNA polymerase since RNA synthesis in ultraviolet-irradiated E. coli cells infected with unirradiated T(4) is sensitive to rifampicin.", "contents": "Ineffectiveness of rifampicin in inhibiting RNA synthesis in Escherichia coli and T(4)-infected Escherichia coli cells after exposure to ultraviolet radiation. Resdiual RNA synthesis in Escherichia coli cells and T(4)-infected E. coli cells exposed to ultraviolet radiation is insensitive to rifampicin. On the other hand, residual RNA synthesis in these cells after gamma irradiation is further inhibited by rifampicin. The specific effect exerted by ultraviolet irradiation on transcription seems to arise from alterations in the DNA structure rather than in bacterial RNA polymerase since RNA synthesis in ultraviolet-irradiated E. coli cells infected with unirradiated T(4) is sensitive to rifampicin."}
{"id": "PMID:1092354", "title": "Tertiary structure in E. coli tRNA Arg and tRNA Val.", "content": "300 MHz proton NMR has been used to demonstrate that both Escherichia coli tRNA Arg and tRNA(1) Val have a tertiary structure base pair between 4-thiouridine (s-4U) at position 8 and A at position 14. Formation of this s-4U(8)-A(14) base pair leads to a very low field (14.8 ppm) resonance in the spectra of both molecules. When s-4U is converted to U the 14.8 resonance is replaced by a new resonance which appears at 14.3 ppm. The presence of the tertiary structure s-4U(8)-A(14) base pair greatly constrains the folding of these tRNAs in solution.", "contents": "Tertiary structure in E. coli tRNA Arg and tRNA Val. 300 MHz proton NMR has been used to demonstrate that both Escherichia coli tRNA Arg and tRNA(1) Val have a tertiary structure base pair between 4-thiouridine (s-4U) at position 8 and A at position 14. Formation of this s-4U(8)-A(14) base pair leads to a very low field (14.8 ppm) resonance in the spectra of both molecules. When s-4U is converted to U the 14.8 resonance is replaced by a new resonance which appears at 14.3 ppm. The presence of the tertiary structure s-4U(8)-A(14) base pair greatly constrains the folding of these tRNAs in solution."}
{"id": "PMID:1092355", "title": "A simple method for the preparation of large quantities of pure plasmid DNA.", "content": "Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures.", "contents": "A simple method for the preparation of large quantities of pure plasmid DNA. Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures."}
{"id": "PMID:1092356", "title": "Studies of insulin release and rat pancreatic islet metabolism with diastereomers of D-glucose.", "content": "Studies of insulin release with diastereomers and other analogues of D-glucose demonstrated that only sugars which undergo oxidation to CO2 stimulated insulin release by the pancreatic islet. None of the non-metabolizable diastereomers of glucose stimulated insulin release in the presence of a sub-stimulatory concentration of glucose for fuel. Although 5.5 mM glucose formed 77% as much CO2 as 16.7 mM mannose and twice that of 16.7 mM fructose, 5.5 mM glucose did not stimulate insulin release whereas 16.7 mM mannose and fructose did stimulate insulin release. These results indicate that the important stimulus for glucose-induced insulin release involves metabolism of glucose, but that the stimulus does not involve solely a fuel function of glucose.", "contents": "Studies of insulin release and rat pancreatic islet metabolism with diastereomers of D-glucose. Studies of insulin release with diastereomers and other analogues of D-glucose demonstrated that only sugars which undergo oxidation to CO2 stimulated insulin release by the pancreatic islet. None of the non-metabolizable diastereomers of glucose stimulated insulin release in the presence of a sub-stimulatory concentration of glucose for fuel. Although 5.5 mM glucose formed 77% as much CO2 as 16.7 mM mannose and twice that of 16.7 mM fructose, 5.5 mM glucose did not stimulate insulin release whereas 16.7 mM mannose and fructose did stimulate insulin release. These results indicate that the important stimulus for glucose-induced insulin release involves metabolism of glucose, but that the stimulus does not involve solely a fuel function of glucose."}
{"id": "PMID:1092357", "title": "The effect of p-aminosalicyclic acid on iron transport and assimilation in mycobacteria.", "content": "p-Aminosalicylic acid inhibits growth of Mycobacterium bovis BCG and Mycobacterium smegmatis more effectively if cells are growing with a sufficiency of iron (more than 1 mu g Fe/ml) in the medium than if cells are deficient in iron (smaller than 0.1 mu g Fe/ml). In iron-deficient cultures formation of mycobactin, an ionophore for iron transport, is strongly inhibited by p-aminosalicylic acid. Uptake of iron into cell suspensions is also inhibited and the activity of several iron-containing enzymes declines in cells exposed to p-aminosalicylic acid during their growth. p-Aminosalicylic acid is about 50 times more effective towards a mutant of M. smegmatis which required mycobactin under iron-deficient growth conditions than towards the wild-type parent. p-Aminosalicylate is taken up into cells by an active process independent of the salicylate uptake system, possibly by the route used for assimilation of p-aminobenzoate. (This could account for why p-aminobenzoic acid, but not salicylic acid, antagonizes the action of p-aminosalicylic acid.) With iron-deficient cells, salicylate assimilation is about 50 times greater than either p-aminosalicylate or p-aminobenzoate but with iron-sufficient cells and with the mycobactin mutant salicylate uptake is negligible whereas p-aminobenzoate and p-aminosalicylate uptakes are unaffected. p-Aminosalicylic acid at 3.3 mM (500 mu g/ml) partially inhibits the uptake of both p-aminobenzoate and, if it is occurring, that of salicylate as well. As p-aminosalicylic acid is always more effective when the intracellular concentration of salicylic acid is low, it probably acts as an anti-metabolite of salicylic acid, not, however, by inhibiting the conversion of salicylic acid to mycobactin, but probably somewhere along the metabolic pathway of iron uptake.", "contents": "The effect of p-aminosalicyclic acid on iron transport and assimilation in mycobacteria. p-Aminosalicylic acid inhibits growth of Mycobacterium bovis BCG and Mycobacterium smegmatis more effectively if cells are growing with a sufficiency of iron (more than 1 mu g Fe/ml) in the medium than if cells are deficient in iron (smaller than 0.1 mu g Fe/ml). In iron-deficient cultures formation of mycobactin, an ionophore for iron transport, is strongly inhibited by p-aminosalicylic acid. Uptake of iron into cell suspensions is also inhibited and the activity of several iron-containing enzymes declines in cells exposed to p-aminosalicylic acid during their growth. p-Aminosalicylic acid is about 50 times more effective towards a mutant of M. smegmatis which required mycobactin under iron-deficient growth conditions than towards the wild-type parent. p-Aminosalicylate is taken up into cells by an active process independent of the salicylate uptake system, possibly by the route used for assimilation of p-aminobenzoate. (This could account for why p-aminobenzoic acid, but not salicylic acid, antagonizes the action of p-aminosalicylic acid.) With iron-deficient cells, salicylate assimilation is about 50 times greater than either p-aminosalicylate or p-aminobenzoate but with iron-sufficient cells and with the mycobactin mutant salicylate uptake is negligible whereas p-aminobenzoate and p-aminosalicylate uptakes are unaffected. p-Aminosalicylic acid at 3.3 mM (500 mu g/ml) partially inhibits the uptake of both p-aminobenzoate and, if it is occurring, that of salicylate as well. As p-aminosalicylic acid is always more effective when the intracellular concentration of salicylic acid is low, it probably acts as an anti-metabolite of salicylic acid, not, however, by inhibiting the conversion of salicylic acid to mycobactin, but probably somewhere along the metabolic pathway of iron uptake."}
{"id": "PMID:1092358", "title": "Structure of the glycopeptide storage material in GM 1 gangliosidosis. Sequence determination with specific endo- and exoglycosidases.", "content": "1. An endo-beta-galactosidase from Escherichia freundii, specific for the hydrolysis of desulfated keratan sulfate, quantitatively liberated a trisaccharide (Gal-GlcNAc-Gal) from a glycopeptide (Mr 1800) isolated from the liver of a patient with GM 1 (generalized) gangliosidosis. 2. The remaining glycopeptide was susceptible to sequential digestion with purified beta-N-acetylhexosaminidase and exo-beta-galactosidase from Jack Bean meal. 3. These and other studies established the structure of the stored glycopeptide to be:(see article) which probably represents the desulfated linkage region of skeletal keratan sulfate.", "contents": "Structure of the glycopeptide storage material in GM 1 gangliosidosis. Sequence determination with specific endo- and exoglycosidases. 1. An endo-beta-galactosidase from Escherichia freundii, specific for the hydrolysis of desulfated keratan sulfate, quantitatively liberated a trisaccharide (Gal-GlcNAc-Gal) from a glycopeptide (Mr 1800) isolated from the liver of a patient with GM 1 (generalized) gangliosidosis. 2. The remaining glycopeptide was susceptible to sequential digestion with purified beta-N-acetylhexosaminidase and exo-beta-galactosidase from Jack Bean meal. 3. These and other studies established the structure of the stored glycopeptide to be:(see article) which probably represents the desulfated linkage region of skeletal keratan sulfate."}
{"id": "PMID:1092359", "title": "The S-n-propyl analogue of S-adenosylmethionine.", "content": "A special strain of Saccharomyces cerevisiae responded to a supplement of S-n-propyl-L-homocysteine in the culture medium by synthesizing S-adenosyl-(S-n-propyl)L-homocysteine, the S-n-propyl analogue of S-adenosylmethionine. S-n-Butyl-L-homocysteine reacted sparingly with this strain, but S-isopropyl-L-homocysteine failed to form detectable quantities of the corresponding S-adenosylsulfonium compound. The S-n-propyl compound was isolated by extraction of the cells, followed by ion-exchange chromatography, which separated it from endogenous S-adenosylmethionine. The structure was determined by hydrolytic procedures leading to overlapping fragments of known structure, 5'-n-propylthioadenosine and S-n-propyl-L-homocysteine. The new sulfonium compound was examined for its activity as n-propyl donor by substituting it for S-adenosylmethionine in methyltransferase systems. Enzymatic transpropylation was observed with S-adenosylmethionine : L-homocysteine S-methyltransferase (EC 2.1.1.10). Its rate was low in the S-adenosylmethionine : N-acetylserotonin O-methyltransferase system (EC 2.1.1.4), and below recognition with S-adenosylmethionine : guanidinoacetate methyltransferase (EC 2.1.1.2) and S-adenosylmethionine : histamine N-methyltransferase (EC 2.1.1.8).", "contents": "The S-n-propyl analogue of S-adenosylmethionine. A special strain of Saccharomyces cerevisiae responded to a supplement of S-n-propyl-L-homocysteine in the culture medium by synthesizing S-adenosyl-(S-n-propyl)L-homocysteine, the S-n-propyl analogue of S-adenosylmethionine. S-n-Butyl-L-homocysteine reacted sparingly with this strain, but S-isopropyl-L-homocysteine failed to form detectable quantities of the corresponding S-adenosylsulfonium compound. The S-n-propyl compound was isolated by extraction of the cells, followed by ion-exchange chromatography, which separated it from endogenous S-adenosylmethionine. The structure was determined by hydrolytic procedures leading to overlapping fragments of known structure, 5'-n-propylthioadenosine and S-n-propyl-L-homocysteine. The new sulfonium compound was examined for its activity as n-propyl donor by substituting it for S-adenosylmethionine in methyltransferase systems. Enzymatic transpropylation was observed with S-adenosylmethionine : L-homocysteine S-methyltransferase (EC 2.1.1.10). Its rate was low in the S-adenosylmethionine : N-acetylserotonin O-methyltransferase system (EC 2.1.1.4), and below recognition with S-adenosylmethionine : guanidinoacetate methyltransferase (EC 2.1.1.2) and S-adenosylmethionine : histamine N-methyltransferase (EC 2.1.1.8)."}
{"id": "PMID:1092360", "title": "Microheterogeneity in yeast invertase.", "content": "Yeast external invertase (EC 3.2.1.25), a glycoenzyme consisting of equal parts by weight of protein and mannan, has been found to contain covalently bound phosphate. Three preparations (from two yeast strains) had mannose/PO4 ratios of 31-35, equivalent to 24-27 PO4 residues per mol of enzyme, while a fourth had only 7 PO4 residues per mol. From one of the high-PO4 enzymes, approx. 69% of the phosphorus was recovered as mannose 6-phosphate. No correlation was found between invertase activity and phosphorus content. The PO4 contents of the invertases exceeded those of the cell wall mannans from the respective yeasts. Thus, contamination of the invertases by cell wall phosphomannan is unlikely. Electrofocusing of the low-PO4 invertase yielded four components with pI values from 3.96 to 4.40, and yeast internal invertase (a mannan- and PO4-free, cytoplasmic isozyme) was isoelectric at approx. pH 4.5. The high-PO4 invertase was considerably more heterogeneous, with two major species of pI 3.65 and 3.32 and a highly acidic component of pI smaller than 2.7; however, the mannose/PO4 ratio of each species was approximately the same. PO4-gradient elution from hydroxyapatite resolved the high-PO4 invertase into five isozymes of increasing acidity and mannan content. Since the mannose/PO4 ratios of these invertase species are constant, the increase in the mannan/protein (and, therefore PO4/protein ratio is apparently responsible for the microheterogeneity of phosphoinvertase.23", "contents": "Microheterogeneity in yeast invertase. Yeast external invertase (EC 3.2.1.25), a glycoenzyme consisting of equal parts by weight of protein and mannan, has been found to contain covalently bound phosphate. Three preparations (from two yeast strains) had mannose/PO4 ratios of 31-35, equivalent to 24-27 PO4 residues per mol of enzyme, while a fourth had only 7 PO4 residues per mol. From one of the high-PO4 enzymes, approx. 69% of the phosphorus was recovered as mannose 6-phosphate. No correlation was found between invertase activity and phosphorus content. The PO4 contents of the invertases exceeded those of the cell wall mannans from the respective yeasts. Thus, contamination of the invertases by cell wall phosphomannan is unlikely. Electrofocusing of the low-PO4 invertase yielded four components with pI values from 3.96 to 4.40, and yeast internal invertase (a mannan- and PO4-free, cytoplasmic isozyme) was isoelectric at approx. pH 4.5. The high-PO4 invertase was considerably more heterogeneous, with two major species of pI 3.65 and 3.32 and a highly acidic component of pI smaller than 2.7; however, the mannose/PO4 ratio of each species was approximately the same. PO4-gradient elution from hydroxyapatite resolved the high-PO4 invertase into five isozymes of increasing acidity and mannan content. Since the mannose/PO4 ratios of these invertase species are constant, the increase in the mannan/protein (and, therefore PO4/protein ratio is apparently responsible for the microheterogeneity of phosphoinvertase.23"}
{"id": "PMID:1092361", "title": "The primary structure of the ribosomal protein L29 from Escherichia coli.", "content": "The amino acid sequence of ribosomal protein L29 was determined utilizing an improved Beckman sequenator, a solid phase peptide sequenator, and more conventional techniques. L29 is composed of 63 amino acid residues and has a molecular weight of 7262. It lacks proline, cysteine, isoleucine, tyrosine and tryptophan. The N-terminal and C-terminal regions (residues 1-9 and 50-63) are basic, while the rest of the molecule is hydrophobic and neutral. Calculated P-a values show two helical regions: one from residues 1 through 32 and the other from residues 37 through 47. No beta-sheet regions are predicted. Short identical regions occur within the sequence of L29 as well as between L29 and other ribosomal proteins.", "contents": "The primary structure of the ribosomal protein L29 from Escherichia coli. The amino acid sequence of ribosomal protein L29 was determined utilizing an improved Beckman sequenator, a solid phase peptide sequenator, and more conventional techniques. L29 is composed of 63 amino acid residues and has a molecular weight of 7262. It lacks proline, cysteine, isoleucine, tyrosine and tryptophan. The N-terminal and C-terminal regions (residues 1-9 and 50-63) are basic, while the rest of the molecule is hydrophobic and neutral. Calculated P-a values show two helical regions: one from residues 1 through 32 and the other from residues 37 through 47. No beta-sheet regions are predicted. Short identical regions occur within the sequence of L29 as well as between L29 and other ribosomal proteins."}
{"id": "PMID:1092362", "title": "Energy requirement for the initiation of colicin action in Escherichia coli.", "content": "1. Starved cells of a strain of Escherichia coli and its mutant uncA, treated with colicin K, E2 or E3, remained fully rescuable upon trypsin treatment (stage I in colicin action). The transition to stage II in colicin action (cells no longer rescuable by trypsin) was promoted by the addition of either glucose or D-lactate. 2. Aerobically glucose-grown cells of the normal strain were irreversibly killed by colicin K, E2 or E3 under anerobic conditions, while similarly treated cells ot its mutant uncA remained fully rescuable. The stage I-stage II transition in colicin action was blocked in normal cells under anaerobic conditions when succinate was the sole carbon source. 3. Arsenate alone had little effect on the progression of the stage I-stage II transition in normal cells, treated with colicin K. However, this transition was abolished in the presence of both arsenate and anaerobic conditions. 4. The initiation of colicin action could be coupled to the anaerobic electron transfer systems formate dehydrogenase-nitrate reductase and alpha-glycerophosphate dehydrogenase-fumarate reductase. 5. These results indicate that an energized state of the cytoplasmic membrane is required for the initiation of colicin action and that no high-energy phosphorylated compounds are necessary.", "contents": "Energy requirement for the initiation of colicin action in Escherichia coli. 1. Starved cells of a strain of Escherichia coli and its mutant uncA, treated with colicin K, E2 or E3, remained fully rescuable upon trypsin treatment (stage I in colicin action). The transition to stage II in colicin action (cells no longer rescuable by trypsin) was promoted by the addition of either glucose or D-lactate. 2. Aerobically glucose-grown cells of the normal strain were irreversibly killed by colicin K, E2 or E3 under anerobic conditions, while similarly treated cells ot its mutant uncA remained fully rescuable. The stage I-stage II transition in colicin action was blocked in normal cells under anaerobic conditions when succinate was the sole carbon source. 3. Arsenate alone had little effect on the progression of the stage I-stage II transition in normal cells, treated with colicin K. However, this transition was abolished in the presence of both arsenate and anaerobic conditions. 4. The initiation of colicin action could be coupled to the anaerobic electron transfer systems formate dehydrogenase-nitrate reductase and alpha-glycerophosphate dehydrogenase-fumarate reductase. 5. These results indicate that an energized state of the cytoplasmic membrane is required for the initiation of colicin action and that no high-energy phosphorylated compounds are necessary."}
{"id": "PMID:1092363", "title": "Substrate activity of phosphonic acid analogues of CDPdiglyceride in the synthesis of phosphoglycerides in Escherichia coli.", "content": "Two phosphonic acid analogues of CDPdiglyceride, D L-2-hexadecoxy-3-octadecoxypropylphosphonyl-O-(cytidine 5'-phosphate) (analogue (I), and D L-3,4-dioctadecoxybutylphosphonyl-O-(cytidine 5'-phosphate) (analogue (II), have been synthesized and examined as substrates for the enzymes involved in the synthesis of phosphoglycerides in Escherichia coli. Both compounds were substrates for CDPdiglyceride:sn-glycerol-3-phosphate phosphatidyl transferase. The analogues had similar Km values (Km of 0.060 mM for analogue (II): Km of 0.080 mM for analogue (I) and a V identical to that of CDPdipalmitin (Km of 0.044 mM). In contrast, the analogues were poor substrates for CDPdiglyceride:L-serine phosphatidyl transferase. The analogues had lower Km values (Km of 0.40 mM for analogue (II); Km of 0.80 mM for analogue (I) than CDPdipalmitin (Km of 1.4 mM). The V, although identical for both analogues, was ten-fold lower than that observed with the natural substrate. An analysis of the products of these enzymatic reactions suggests that phosphatidylglycerophosphate phosphatase and phosphatidylserine decarboxylase may also possess a certain degree of substrate specificity.", "contents": "Substrate activity of phosphonic acid analogues of CDPdiglyceride in the synthesis of phosphoglycerides in Escherichia coli. Two phosphonic acid analogues of CDPdiglyceride, D L-2-hexadecoxy-3-octadecoxypropylphosphonyl-O-(cytidine 5'-phosphate) (analogue (I), and D L-3,4-dioctadecoxybutylphosphonyl-O-(cytidine 5'-phosphate) (analogue (II), have been synthesized and examined as substrates for the enzymes involved in the synthesis of phosphoglycerides in Escherichia coli. Both compounds were substrates for CDPdiglyceride:sn-glycerol-3-phosphate phosphatidyl transferase. The analogues had similar Km values (Km of 0.060 mM for analogue (II): Km of 0.080 mM for analogue (I) and a V identical to that of CDPdipalmitin (Km of 0.044 mM). In contrast, the analogues were poor substrates for CDPdiglyceride:L-serine phosphatidyl transferase. The analogues had lower Km values (Km of 0.40 mM for analogue (II); Km of 0.80 mM for analogue (I) than CDPdipalmitin (Km of 1.4 mM). The V, although identical for both analogues, was ten-fold lower than that observed with the natural substrate. An analysis of the products of these enzymatic reactions suggests that phosphatidylglycerophosphate phosphatase and phosphatidylserine decarboxylase may also possess a certain degree of substrate specificity."}
{"id": "PMID:1092364", "title": "Mitochondrial and cellular inhibition by the folate analogue pyrimethamine in Saccharomyces cerevisiae: reversal of cellular effects by TMP.", "content": "The inhibitory effect of pyrimethamine on the growth of TMP-permeable strains of Saccharomyces cerevisiae in a fermentable medium supplemented with adenine, glycine, methionine and pantothenate was substantially reduced by exogenous TMP. This compound also suppressed the drug's killing effect, and to some extent its ability to induce the mitochondrial petite mutation. In a non-fermentable medium, TMP failed to reduce growth inhibition, in line with our earlier finding that as well as blocking synthesis pyrimethamine prevents mitochondrial protein synthesis.", "contents": "Mitochondrial and cellular inhibition by the folate analogue pyrimethamine in Saccharomyces cerevisiae: reversal of cellular effects by TMP. The inhibitory effect of pyrimethamine on the growth of TMP-permeable strains of Saccharomyces cerevisiae in a fermentable medium supplemented with adenine, glycine, methionine and pantothenate was substantially reduced by exogenous TMP. This compound also suppressed the drug's killing effect, and to some extent its ability to induce the mitochondrial petite mutation. In a non-fermentable medium, TMP failed to reduce growth inhibition, in line with our earlier finding that as well as blocking synthesis pyrimethamine prevents mitochondrial protein synthesis."}
{"id": "PMID:1092365", "title": "Synthesis of heteropolyribonucleotides by toluene-treated Escherichia coli cells.", "content": "Escherichia coli cells, made permeable to ribonucleoside-5'-diphosphates by treatment with toluene, efficiently promote the synthesis of homo- and heteropolynucleotides. This synthesis is catalyzed by polynucleotide phosphorylase because, among other things, it is inhibited by orthophosphate, and E. coli Q13, a mutant having a Mn-2+-dependent polynucleotide phosphorylase, promotes polynucleotide synthesis in the presence of Mn-2+ but not of Mg-2+. Cells of E. coli B and E. coli MRE 600 (A Mutant lacking ribonuclease I) are about equally active in promoting poly(A, U, G, C) synthesis. Sucrose density gradient and agarose gel electrophoretic analysis of the product show that it is polydisperse with sedimentation coefficients ranging between 4 S and 27 S. The synthesized polynucleotides can be translated by the toluene-treated cells.", "contents": "Synthesis of heteropolyribonucleotides by toluene-treated Escherichia coli cells. Escherichia coli cells, made permeable to ribonucleoside-5'-diphosphates by treatment with toluene, efficiently promote the synthesis of homo- and heteropolynucleotides. This synthesis is catalyzed by polynucleotide phosphorylase because, among other things, it is inhibited by orthophosphate, and E. coli Q13, a mutant having a Mn-2+-dependent polynucleotide phosphorylase, promotes polynucleotide synthesis in the presence of Mn-2+ but not of Mg-2+. Cells of E. coli B and E. coli MRE 600 (A Mutant lacking ribonuclease I) are about equally active in promoting poly(A, U, G, C) synthesis. Sucrose density gradient and agarose gel electrophoretic analysis of the product show that it is polydisperse with sedimentation coefficients ranging between 4 S and 27 S. The synthesized polynucleotides can be translated by the toluene-treated cells."}
{"id": "PMID:1092368", "title": "Gene activation in human diploid cells. Age-dependent modifications in the stability of messenger RNAs for nonhistone chromosomal proteins.", "content": "Serum stimulation of early as well as late passages of nondividing WI-38 human diploid fibroblasts to proliferate, results in DNA synthesis beginning at 12 hours and MITOSIS AT 20 HOURS. A 2-fold increase in the transcriptional activity of chromatin isolated from early and late passage W2-38 cells is evident one hour following serum stimulation. An increased synthesis and association with the genome of two defined molecular weight classes of nonhistone chromosomal proteins one hour following serum stimulation of early and late passage cells is also observed. The increased chromatin template activity and nonhistone chromosomal protein synthesis occur in early passage cells stimulated to proliferate in the presence of actinomycin D. However, when late passage WI-38 cells are stimulated in the presence of antinomycin D, increases in chromatin template activity and nonhistone chromosomal protein synthesis are not observed one hour following serum stimulation. The possibility that nonhistone chromosomal protein synthesis and activation of transcription early during the prereplicative phase of the cell cycle in early passage human diploid fibroblasts may be regulated at the translational level is discussed. Consideration is also given to the possibility that there may be an age-dependent modification in such a regulatory mechanism.", "contents": "Gene activation in human diploid cells. Age-dependent modifications in the stability of messenger RNAs for nonhistone chromosomal proteins. Serum stimulation of early as well as late passages of nondividing WI-38 human diploid fibroblasts to proliferate, results in DNA synthesis beginning at 12 hours and MITOSIS AT 20 HOURS. A 2-fold increase in the transcriptional activity of chromatin isolated from early and late passage W2-38 cells is evident one hour following serum stimulation. An increased synthesis and association with the genome of two defined molecular weight classes of nonhistone chromosomal proteins one hour following serum stimulation of early and late passage cells is also observed. The increased chromatin template activity and nonhistone chromosomal protein synthesis occur in early passage cells stimulated to proliferate in the presence of actinomycin D. However, when late passage WI-38 cells are stimulated in the presence of antinomycin D, increases in chromatin template activity and nonhistone chromosomal protein synthesis are not observed one hour following serum stimulation. The possibility that nonhistone chromosomal protein synthesis and activation of transcription early during the prereplicative phase of the cell cycle in early passage human diploid fibroblasts may be regulated at the translational level is discussed. Consideration is also given to the possibility that there may be an age-dependent modification in such a regulatory mechanism."}
{"id": "PMID:1092369", "title": "The effect of double-stranded cowpea mosaic viral RNA on protein synthesis.", "content": "The effect of double-stranded cowpea mosaic viral RNA on several in vitro protein synthesizing systems was studied. No inhibitory effect of this RNA at concentrations up to 16 mug/ml was found in wheat germ protein synthesis programmed with cowpea mosaic viral-, alfalfa mosaic viral- or globin 9 S-RNA. At high concentrations of this double-stranded RNA, inhibition of Escherichia coli in vitro protein synthesis programmed with MS-2- and cowpea mosaic viral-RNA was found, whereas at low concentrations its inhibitory effect on rabbit reticulocyte in vitro protein synthesis was strong. The implications of these observations for the suggestions of other authors, based on the study of mammalian protein synthesizing systems, are discussed.", "contents": "The effect of double-stranded cowpea mosaic viral RNA on protein synthesis. The effect of double-stranded cowpea mosaic viral RNA on several in vitro protein synthesizing systems was studied. No inhibitory effect of this RNA at concentrations up to 16 mug/ml was found in wheat germ protein synthesis programmed with cowpea mosaic viral-, alfalfa mosaic viral- or globin 9 S-RNA. At high concentrations of this double-stranded RNA, inhibition of Escherichia coli in vitro protein synthesis programmed with MS-2- and cowpea mosaic viral-RNA was found, whereas at low concentrations its inhibitory effect on rabbit reticulocyte in vitro protein synthesis was strong. The implications of these observations for the suggestions of other authors, based on the study of mammalian protein synthesizing systems, are discussed."}
{"id": "PMID:1092370", "title": "Stimulation of insulin release by thiols.", "content": "The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob micemin control experiments the reactivity of thiols against 6,6'-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mMD-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the beta-cells.", "contents": "Stimulation of insulin release by thiols. The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob micemin control experiments the reactivity of thiols against 6,6'-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mMD-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the beta-cells."}
{"id": "PMID:1092371", "title": "Stereoselective reduction of cobalt(III) complexes by bacteria.", "content": "A strain of Enterobacter cloacae, isolated from soil, reduces racemic abdtriglycinatocobalt(III), triglycinatocobalt(III), stereoselectively under anaerobic conditions; Washed suspensions oc cells, grown anaerobically in the absence of triglycinatocobalt(III) initially reduce the L-enantiomer stereospecifically under argon yielding a labile Co(II) chelate. An approximately 5-fold increase in the rate of reduction of the complex occurs using cells disrupted by 2-phenylethanol; decomposition now proceeds with a slight preference for the D-enantiomermreduction of triglycinatocobalt(III) under argon by cell-free extracts, prepared by sonication, exhibits similar stereoselectivities to cells disrupted by 2-phenylethanolmhowever, extracts contain a factor capable of reducing D-triglycinatocobalt(III) with high stereoselectivity under molecular hydrogenmthe behaviour of Enterobacter cloacae is compared with that of Aerobacter aerogenes, Proteus vulgaris, Escherichia anaerogenes and Serratia marcescensmstereoselective effects are also observed in the bacterial reduction of other cobalt(III) complexes.", "contents": "Stereoselective reduction of cobalt(III) complexes by bacteria. A strain of Enterobacter cloacae, isolated from soil, reduces racemic abdtriglycinatocobalt(III), triglycinatocobalt(III), stereoselectively under anaerobic conditions; Washed suspensions oc cells, grown anaerobically in the absence of triglycinatocobalt(III) initially reduce the L-enantiomer stereospecifically under argon yielding a labile Co(II) chelate. An approximately 5-fold increase in the rate of reduction of the complex occurs using cells disrupted by 2-phenylethanol; decomposition now proceeds with a slight preference for the D-enantiomermreduction of triglycinatocobalt(III) under argon by cell-free extracts, prepared by sonication, exhibits similar stereoselectivities to cells disrupted by 2-phenylethanolmhowever, extracts contain a factor capable of reducing D-triglycinatocobalt(III) with high stereoselectivity under molecular hydrogenmthe behaviour of Enterobacter cloacae is compared with that of Aerobacter aerogenes, Proteus vulgaris, Escherichia anaerogenes and Serratia marcescensmstereoselective effects are also observed in the bacterial reduction of other cobalt(III) complexes."}
{"id": "PMID:1092372", "title": "Mode of action of the cloacin DF13-immunity protein.", "content": "1. Cells of Enterobacter cloacae harbouring the bacteriocinogenic factor Clo DF13 produce an immunity protein which inbhbits the in vitro activity of cloacin DF13. The amino acid composition of purified immunity protein was determined. 2. Experiments about the protection of ribosomes against cloacin DF13 in the presence of the immunity protein show that one molecule of immunity protein neutralized the activity of one molecule cloacin. 3. Direct and specific interaction of cloacin DF13 with the immunity protein has been demonstrated by the analysis of mixtures of both proteins on polyacrylamide gels and by changes in the fluorescence response of cloacin DF13-bound 1-anilinonaphthalene-8-sulfonate in the presence of immunity protein.", "contents": "Mode of action of the cloacin DF13-immunity protein. 1. Cells of Enterobacter cloacae harbouring the bacteriocinogenic factor Clo DF13 produce an immunity protein which inbhbits the in vitro activity of cloacin DF13. The amino acid composition of purified immunity protein was determined. 2. Experiments about the protection of ribosomes against cloacin DF13 in the presence of the immunity protein show that one molecule of immunity protein neutralized the activity of one molecule cloacin. 3. Direct and specific interaction of cloacin DF13 with the immunity protein has been demonstrated by the analysis of mixtures of both proteins on polyacrylamide gels and by changes in the fluorescence response of cloacin DF13-bound 1-anilinonaphthalene-8-sulfonate in the presence of immunity protein."}
{"id": "PMID:1092373", "title": "Preparation, properties and biological activities of succinyl derivatives of vitamin B12.", "content": "Three new derivatives of vitamin B12,0-2'-succinyl-, 0-5'-succinyl-, and 0-2', 0-5'-disuccinyl-vitamin B12, whose alpha-ribose moieties of the nucleotide ligand are succinylated, were prepared by reaction of the vitamin with succinic anhydride. The first succinylation took place rapidly and almost predominantly on 5'-OH of alpha-ribose, and the second succinylation much more slowly on 2'-OH. From the behaviors in paper electrophoresis and the lability to CH- of cobalt-base bond of 0-2'-succinylated vitamin B12 derivatives, it was suggested that the terminal COOH of the 0-2'-succinyl group forms an inner salt with the imidazole nucleus of 5,6-dimethylbenzimidazole. Monosuccinyland disuccinyl-vitamin B12 by mild acid or base hydrolysis. Heating at 130 degrees for 5 min also led to the complete severance of the succinyl group of 0-5'-succinyl-vitamin B12. None of the three succinly derivatives inhibited the diol dehydrase reaction when added with coenzyme B12, SUggesting that the ability to bind to the apoenzyme is strongly diminished or almost lost by succinylation on 2'- or 5'-OH of alpha-ribose. None of the succinyl vitamin B12 compounds showed either biological activity or anti-vitamin B12 activity when tested with Escherichia coli 215, a methionine-B12 auxotroph. None of them significantly inhibited [3-H] vitamin B12 uptake by E. coli 215 cells. This observation implies that succinyl derivatives of vitamin B12 are hardly incorporated into the cells of E. coli.", "contents": "Preparation, properties and biological activities of succinyl derivatives of vitamin B12. Three new derivatives of vitamin B12,0-2'-succinyl-, 0-5'-succinyl-, and 0-2', 0-5'-disuccinyl-vitamin B12, whose alpha-ribose moieties of the nucleotide ligand are succinylated, were prepared by reaction of the vitamin with succinic anhydride. The first succinylation took place rapidly and almost predominantly on 5'-OH of alpha-ribose, and the second succinylation much more slowly on 2'-OH. From the behaviors in paper electrophoresis and the lability to CH- of cobalt-base bond of 0-2'-succinylated vitamin B12 derivatives, it was suggested that the terminal COOH of the 0-2'-succinyl group forms an inner salt with the imidazole nucleus of 5,6-dimethylbenzimidazole. Monosuccinyland disuccinyl-vitamin B12 by mild acid or base hydrolysis. Heating at 130 degrees for 5 min also led to the complete severance of the succinyl group of 0-5'-succinyl-vitamin B12. None of the three succinly derivatives inhibited the diol dehydrase reaction when added with coenzyme B12, SUggesting that the ability to bind to the apoenzyme is strongly diminished or almost lost by succinylation on 2'- or 5'-OH of alpha-ribose. None of the succinyl vitamin B12 compounds showed either biological activity or anti-vitamin B12 activity when tested with Escherichia coli 215, a methionine-B12 auxotroph. None of them significantly inhibited [3-H] vitamin B12 uptake by E. coli 215 cells. This observation implies that succinyl derivatives of vitamin B12 are hardly incorporated into the cells of E. coli."}
{"id": "PMID:1092374", "title": "Cardiac output measurement by indicator dilution.", "content": "The measurement of cardiac output by the indicator dilution method is reviewed. Theoretical and practical problems relating to the direct and indirect Fick methods and the continuous and bolus injection methods are considered in detail. Choice of indicator is also discussed. Problems remain with all of these methods, but reasonable accuracy and consistency may be achieved if attention is paid to detail.", "contents": "Cardiac output measurement by indicator dilution. The measurement of cardiac output by the indicator dilution method is reviewed. Theoretical and practical problems relating to the direct and indirect Fick methods and the continuous and bolus injection methods are considered in detail. Choice of indicator is also discussed. Problems remain with all of these methods, but reasonable accuracy and consistency may be achieved if attention is paid to detail."}
{"id": "PMID:1092381", "title": "Nerve excitability--toward an integrating concept.", "content": "Although numerous experimental data have been accumulated in the various fields of research on bioelectricity, the mechanism of nerve excitability is still an unsolved problem. Many mechanistic interpretations of nerve behavior cover only a part of the facts, are thus selective and unsatisfactory. An attempt at an integral interpretation of basic data well-established by electrophysiological, biochemical, and biophysical investigations was inspired by the late Aharon Katchalsky and a first attempt had been made previously (Neumann et al., 1973). The present account is a further step toward a quantitative physiochemical theory of bioelectricity. We have further explored the previously introduced notion of a basic excitation unit in excitable membranes. This notion is of fundamental importance for modeling details of sub- and suprathreshold responses, such as threshold behavior and strength-duration curves, in terms of kinetic parameters for specific membrane processes. Our integral model of excitability is based on the original chemical hypothesis for the control of bioelectricity (Nachmansohn, 1959, 1971b). This specific approach includes some frequently ignored experimental facts on acetylcholine-processing proteins in excitable membranes. According to the integral model, acetylcholine ions are continuously processed through the basic excitation units within excitable membranes: axonal, presynaptic, and postsynaptic parts. Excitability, i.e., the generation and propagation of nerve impulses, is due to a cooperative increase in the rate of AcCh translocation through the cholinergic control system.", "contents": "Nerve excitability--toward an integrating concept. Although numerous experimental data have been accumulated in the various fields of research on bioelectricity, the mechanism of nerve excitability is still an unsolved problem. Many mechanistic interpretations of nerve behavior cover only a part of the facts, are thus selective and unsatisfactory. An attempt at an integral interpretation of basic data well-established by electrophysiological, biochemical, and biophysical investigations was inspired by the late Aharon Katchalsky and a first attempt had been made previously (Neumann et al., 1973). The present account is a further step toward a quantitative physiochemical theory of bioelectricity. We have further explored the previously introduced notion of a basic excitation unit in excitable membranes. This notion is of fundamental importance for modeling details of sub- and suprathreshold responses, such as threshold behavior and strength-duration curves, in terms of kinetic parameters for specific membrane processes. Our integral model of excitability is based on the original chemical hypothesis for the control of bioelectricity (Nachmansohn, 1959, 1971b). This specific approach includes some frequently ignored experimental facts on acetylcholine-processing proteins in excitable membranes. According to the integral model, acetylcholine ions are continuously processed through the basic excitation units within excitable membranes: axonal, presynaptic, and postsynaptic parts. Excitability, i.e., the generation and propagation of nerve impulses, is due to a cooperative increase in the rate of AcCh translocation through the cholinergic control system."}
{"id": "PMID:1092382", "title": "Anomalous sedimentation behaviour of the E.coli ribosomal system: 50S-30S forms 50S PLUS 30S.", "content": "An experimenal and theoretical study has been made into the effect of association-dissociation reactions on the sedimentation of the E.coli ribosomal 50S-30S forms 50S plus 30S. It has been found that (a) the sedimentation pattern is strongly dependent on the rotor speed: (b) the ratio of components as measured using high-speed ultracentrifugation (30000-40000 r.p.m.) is independent of rotor speed; and (c) the speed of ultracentrifugation has a strong effect on the sedimentation coefficeint of the ribosomal system as determined by the mean square second moment. The results of this paper demonstrate that ribosome sedimentation at low-speed ultracentrifugation is affected by some artefactual processes. A theoretical analysis of the experimental findings has shown that the observed effects cannot be attributed to the effect of the association-dissociation reaction nor to the pressure dependence of the equilibrium constant of that reaction. On the other hand, at high-speed ultracentrifugation the ribosomal system sediments as a heterogeneous mixture of non-interacting components. Consequently, the shape of the boundary in this case will reflect the equilibrium composition of the ribosomal system.", "contents": "Anomalous sedimentation behaviour of the E.coli ribosomal system: 50S-30S forms 50S PLUS 30S. An experimenal and theoretical study has been made into the effect of association-dissociation reactions on the sedimentation of the E.coli ribosomal 50S-30S forms 50S plus 30S. It has been found that (a) the sedimentation pattern is strongly dependent on the rotor speed: (b) the ratio of components as measured using high-speed ultracentrifugation (30000-40000 r.p.m.) is independent of rotor speed; and (c) the speed of ultracentrifugation has a strong effect on the sedimentation coefficeint of the ribosomal system as determined by the mean square second moment. The results of this paper demonstrate that ribosome sedimentation at low-speed ultracentrifugation is affected by some artefactual processes. A theoretical analysis of the experimental findings has shown that the observed effects cannot be attributed to the effect of the association-dissociation reaction nor to the pressure dependence of the equilibrium constant of that reaction. On the other hand, at high-speed ultracentrifugation the ribosomal system sediments as a heterogeneous mixture of non-interacting components. Consequently, the shape of the boundary in this case will reflect the equilibrium composition of the ribosomal system."}
{"id": "PMID:1092383", "title": "High pressure effects on the activity of glycolytic enzymes.", "content": "High hydrostatic pressure inhibits growth in most organisms; this may be explained by a deactivation of enzymes involved in essential metabolic pathways. In order to check this hypothesis the enzymic activity of rabbit muscle lactic dehydrogenase and yeast glyceraldehyde-3-phosphate dehydrogenase was investigated in the presence of the coenzyme and excess of substrate at pressures up to 2kbar. Kinetic analysis of an initial phase of pressure induced activation and of a second phase of reversible deactivation shows that the two enzymes respond to high pressures in different ways leading to a volume of activation of increment V is not equal to (LDH) equal 0 plus or minus 1 cm-3 mol-1 and increment V is not equal to (GAPDH) equals 60 plus or minus 4 cm-3 mol-1, respectively. Comparing the lower limits of pressure deactivation, LDH is found to be more stable towards pressure than GAPDH. At p is approximately equal to 2 kbar total deactivation of both enzymes is observed. A concentration dependent lag of GAPDH reactivation proves dissocation to participate in the process of deactivation, while the effects for LDH are explicable on the basis of reversible denaturation alone.", "contents": "High pressure effects on the activity of glycolytic enzymes. High hydrostatic pressure inhibits growth in most organisms; this may be explained by a deactivation of enzymes involved in essential metabolic pathways. In order to check this hypothesis the enzymic activity of rabbit muscle lactic dehydrogenase and yeast glyceraldehyde-3-phosphate dehydrogenase was investigated in the presence of the coenzyme and excess of substrate at pressures up to 2kbar. Kinetic analysis of an initial phase of pressure induced activation and of a second phase of reversible deactivation shows that the two enzymes respond to high pressures in different ways leading to a volume of activation of increment V is not equal to (LDH) equal 0 plus or minus 1 cm-3 mol-1 and increment V is not equal to (GAPDH) equals 60 plus or minus 4 cm-3 mol-1, respectively. Comparing the lower limits of pressure deactivation, LDH is found to be more stable towards pressure than GAPDH. At p is approximately equal to 2 kbar total deactivation of both enzymes is observed. A concentration dependent lag of GAPDH reactivation proves dissocation to participate in the process of deactivation, while the effects for LDH are explicable on the basis of reversible denaturation alone."}
{"id": "PMID:1092386", "title": "Defective mononuclear leukocyte chemotaxis in the Chediak-Higashi syndrome of humans, mink, and cattle.", "content": "Chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic Boyden chamber technique and a new 51-Cr-labeled mononuclear radioassay with a double micropore filter system. Significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle Chediak-Higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. A similar Chediak-Higashi mononuclear leukocyte defect was noted in humans when kallikrein or dialyzable transfer factor were used as the chemotactic stimulus. Studies using smaller pore filters in the chemotactic chamber exaggerated the chemotactic defect. Serum from Chediak-Higashi subjects had normal chemotactic activity. Additional studies on the spontaneous (random) locomotion of Chediak-Higashi mononuclear leukocytes revealed normal results when a capillary tube assay system was used, but abnormal results were obtained when a Boyden chamber micropore filter assay was used, demonstrating fundamental differences in these two assays of random locomotion. It is clear from these studies that defective mononuclear leukocyte chemotaxis is another feature of the imparied host defenses in the Chediak-Higashi syndrome that may contribute to the marked susceptibility to pyogenic infections so characteristic of this dease.", "contents": "Defective mononuclear leukocyte chemotaxis in the Chediak-Higashi syndrome of humans, mink, and cattle. Chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic Boyden chamber technique and a new 51-Cr-labeled mononuclear radioassay with a double micropore filter system. Significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle Chediak-Higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. A similar Chediak-Higashi mononuclear leukocyte defect was noted in humans when kallikrein or dialyzable transfer factor were used as the chemotactic stimulus. Studies using smaller pore filters in the chemotactic chamber exaggerated the chemotactic defect. Serum from Chediak-Higashi subjects had normal chemotactic activity. Additional studies on the spontaneous (random) locomotion of Chediak-Higashi mononuclear leukocytes revealed normal results when a capillary tube assay system was used, but abnormal results were obtained when a Boyden chamber micropore filter assay was used, demonstrating fundamental differences in these two assays of random locomotion. It is clear from these studies that defective mononuclear leukocyte chemotaxis is another feature of the imparied host defenses in the Chediak-Higashi syndrome that may contribute to the marked susceptibility to pyogenic infections so characteristic of this dease."}
{"id": "PMID:1092389", "title": "Further experience with lateral wedge resection mammaplasties.", "content": "The lateral wedge resection method of reduction mammaplasty results in breasts with a single oblique radial scar in the outer quadrant, good contour, the nipples with sensation, erectile capacity and forward projection. It is suitable for cases of moderate enlargement and for ptotic breasts.", "contents": "Further experience with lateral wedge resection mammaplasties. The lateral wedge resection method of reduction mammaplasty results in breasts with a single oblique radial scar in the outer quadrant, good contour, the nipples with sensation, erectile capacity and forward projection. It is suitable for cases of moderate enlargement and for ptotic breasts."}
{"id": "PMID:1092391", "title": "Relay transplantation: a new method of expanding a free skin graft.", "content": "A method of skin transplantation is presented whereby a wound may be successively sown with epithelium by the repeated transfer of the same strips of split thickness skin.", "contents": "Relay transplantation: a new method of expanding a free skin graft. A method of skin transplantation is presented whereby a wound may be successively sown with epithelium by the repeated transfer of the same strips of split thickness skin."}
{"id": "PMID:1092393", "title": "The back tube pedicle in head and neck reconstruction.", "content": "The use of a back tube pedicle in 19 patients, presenting for reconstruction of defects in the head and neck region, is shown and aspects of surgical technique discussed. In general, satisfactory results were achieved and it is recommended that the back should be considered more often as a donor site when the more convenient deltopectoral flap is not available, not desirable.", "contents": "The back tube pedicle in head and neck reconstruction. The use of a back tube pedicle in 19 patients, presenting for reconstruction of defects in the head and neck region, is shown and aspects of surgical technique discussed. In general, satisfactory results were achieved and it is recommended that the back should be considered more often as a donor site when the more convenient deltopectoral flap is not available, not desirable."}
{"id": "PMID:1092395", "title": "Somersault flap for reconstruction of the helix.", "content": "An original technique for reconstruction of the helix has been successfully applied. Posterior auricular skin ia attached to the medical aspect of the helical rim, then somersaulted laterally into position, recreating the helical curl.", "contents": "Somersault flap for reconstruction of the helix. An original technique for reconstruction of the helix has been successfully applied. Posterior auricular skin ia attached to the medical aspect of the helical rim, then somersaulted laterally into position, recreating the helical curl."}
{"id": "PMID:1092397", "title": "Lip-plug carcinoma and its management by modified Abbe flap.", "content": "From Tanzania 2 cases are reported, and reference made to a third, of carcinoma arising in the upper lip in relation to a lip-plug, a traditional wooden adornment worm within an orifice in the tissues of the upper lip. Mangement was by excision and repair with a modified Abbe flap.", "contents": "Lip-plug carcinoma and its management by modified Abbe flap. From Tanzania 2 cases are reported, and reference made to a third, of carcinoma arising in the upper lip in relation to a lip-plug, a traditional wooden adornment worm within an orifice in the tissues of the upper lip. Mangement was by excision and repair with a modified Abbe flap."}
{"id": "PMID:1092398", "title": "Lithium in non-manic-depressives: antiaggressive effect and red blood cell lithium values.", "content": "Lithium was given to eight aggressive, non-manic-depressive female defectives in a doubleblind placebo-controlled study. The group as a whole showed a reduction in aggression scores while on lithium (p greater than 0.01): three patients became less aggressive,one became worse and two were unchanged. Both affective and predatory aggression seemed to be reduced. Two patients had to be withdrawn from the trial at an early stage because of the development of neurotoxicity.", "contents": "Lithium in non-manic-depressives: antiaggressive effect and red blood cell lithium values. Lithium was given to eight aggressive, non-manic-depressive female defectives in a doubleblind placebo-controlled study. The group as a whole showed a reduction in aggression scores while on lithium (p greater than 0.01): three patients became less aggressive,one became worse and two were unchanged. Both affective and predatory aggression seemed to be reduced. Two patients had to be withdrawn from the trial at an early stage because of the development of neurotoxicity."}
{"id": "PMID:1092399", "title": "The lesion in stereotactic suscaudate tractotomy.", "content": "The anatomical distribution of 25 stereotactic tractotomy lesions is described. The posterior half of these lesions lie in a subcaudate position and the anterior half, for the most part, lies beneath the central segment of frontal white matter.", "contents": "The lesion in stereotactic suscaudate tractotomy. The anatomical distribution of 25 stereotactic tractotomy lesions is described. The posterior half of these lesions lie in a subcaudate position and the anterior half, for the most part, lies beneath the central segment of frontal white matter."}
{"id": "PMID:1092401", "title": "Mathematical method to utilize a computer for diagnosis of site and type of intracerebral mass lesions.", "content": "A mathematical process has been devised which can utilize full clinical data of symptoms and signs, neuroradiological procedures, EEG and isotope scanning to predict the site and pathological process in a patient presenting with disturbance of central nervous system function. The work is a further expansion and modification of the computer program described by Du Boulay and Price (1968).", "contents": "Mathematical method to utilize a computer for diagnosis of site and type of intracerebral mass lesions. A mathematical process has been devised which can utilize full clinical data of symptoms and signs, neuroradiological procedures, EEG and isotope scanning to predict the site and pathological process in a patient presenting with disturbance of central nervous system function. The work is a further expansion and modification of the computer program described by Du Boulay and Price (1968)."}
{"id": "PMID:1092402", "title": "Movement of criticall ill patients within hospital.", "content": "Critically ill patients were observed during routine movement inside the hospital to and from the intensive therapy unit. One patient a month suffered major cardiorespiratory collapse or death as a direct result of movement. Renewed bleeding of a pelvic fracture, cardiac arrhythmia, cardiac embarrassment due to a haemothorax, and cardiovascular decompensation were seen. It was difficult to continue treatment during movement, especially maintaining an airway or providing adequate intermittent positive pressure ventilation. Seventy postoperative patients suffered few ill effects on being moved. Greater awareness of the dangers of moving critically ill patients within hospital is needed. Thorough preparation for the move and adequate maintenance of treatment during movement requires the skill of experienced medical staff.", "contents": "Movement of criticall ill patients within hospital. Critically ill patients were observed during routine movement inside the hospital to and from the intensive therapy unit. One patient a month suffered major cardiorespiratory collapse or death as a direct result of movement. Renewed bleeding of a pelvic fracture, cardiac arrhythmia, cardiac embarrassment due to a haemothorax, and cardiovascular decompensation were seen. It was difficult to continue treatment during movement, especially maintaining an airway or providing adequate intermittent positive pressure ventilation. Seventy postoperative patients suffered few ill effects on being moved. Greater awareness of the dangers of moving critically ill patients within hospital is needed. Thorough preparation for the move and adequate maintenance of treatment during movement requires the skill of experienced medical staff."}
{"id": "PMID:1092407", "title": "Untoward effects associated with practolol: demonstration of antibody binding to epithelial tissue.", "content": "An antibody which sticks to the intercellular region of xenogenic epidermal tissue has been shown by indirect immunofluorescence to be present in the serum of patients with practolol-induced eye damage. These antibodies and those found in patients with pemphigus were compared for their ability to bind to isolated epidermal cells. Binding was achieved only with the pemphigus antibody, which suggests that it may have a different specificity from the antibody associated with practolol-induced eye damage.", "contents": "Untoward effects associated with practolol: demonstration of antibody binding to epithelial tissue. An antibody which sticks to the intercellular region of xenogenic epidermal tissue has been shown by indirect immunofluorescence to be present in the serum of patients with practolol-induced eye damage. These antibodies and those found in patients with pemphigus were compared for their ability to bind to isolated epidermal cells. Binding was achieved only with the pemphigus antibody, which suggests that it may have a different specificity from the antibody associated with practolol-induced eye damage."}
{"id": "PMID:1092408", "title": "Prolactin studies in \"functionless\" pituitary tumours.", "content": "Hyperprolactinaemia was found in all 17 women and in one out of six men who presented with hypogonadism and a radiologically enlarged sella turcica but no other clinical endocrine dysfunction. Some of the women also had galactorrhoea. The greater the level of hyperprolactinaemia in these 18 patients the larger their sellae turcica except in two patients with unusual features. The sella turcica was usually asymmetrically enlarged and there was rearly an upward extension of tumour, though the sella floor often showed some erosion on tomography. An oral dose of bromocriptine suppressed the hyperprolactinaemia in mose patients at the same rate as in normal post-partum women. Nine of the 18 patients with hyperprolactinaemia had low basal luteinizing hormone (LH) levels. The LH responsiveness to 100 mug of LH-releasing hormone (LHRH) was tested in 12, and eight showed subnormal values. Of eight biopsy specimens obtained four showed acidophil granules on light microscopy, and in five granules of various sizes were seen on electron microscopy.", "contents": "Prolactin studies in \"functionless\" pituitary tumours. Hyperprolactinaemia was found in all 17 women and in one out of six men who presented with hypogonadism and a radiologically enlarged sella turcica but no other clinical endocrine dysfunction. Some of the women also had galactorrhoea. The greater the level of hyperprolactinaemia in these 18 patients the larger their sellae turcica except in two patients with unusual features. The sella turcica was usually asymmetrically enlarged and there was rearly an upward extension of tumour, though the sella floor often showed some erosion on tomography. An oral dose of bromocriptine suppressed the hyperprolactinaemia in mose patients at the same rate as in normal post-partum women. Nine of the 18 patients with hyperprolactinaemia had low basal luteinizing hormone (LH) levels. The LH responsiveness to 100 mug of LH-releasing hormone (LHRH) was tested in 12, and eight showed subnormal values. Of eight biopsy specimens obtained four showed acidophil granules on light microscopy, and in five granules of various sizes were seen on electron microscopy."}
{"id": "PMID:1092411", "title": "Prophylaxis of postoperative leg vine thrombosis by low dose subcutaneous heparin or peroperative calf muscle stimulation: a controlled clinical trial.", "content": "In a randomized, controlled clinical trial of two methods of preventing postoperative leg vein thrombosis patients undergoing major surgery were divided into three groups. One received intermittent electrical calf muscle stimulation during surgery, the second subcutaneous heparin calcium 5000 IU every eight hours for six days, and the third no specific prophylaxis. Leg vein thrombosis was detected by the 125-I-fibrinogen uptake test. Neither method was effective in patients undergoing open bladder or prostatic surgery. Stimulation did not reduce the incidence of leg vein thrombosis in patients with malignant disease undergoing laparotomy, but heparin calcium was highly successful in this group (P smaller than 0-001). When the laparotomy was for a benign condition, however, both heparin calcium (P smaller than 0-001) and stimulation (P smaller than 0-01) were effective.", "contents": "Prophylaxis of postoperative leg vine thrombosis by low dose subcutaneous heparin or peroperative calf muscle stimulation: a controlled clinical trial. In a randomized, controlled clinical trial of two methods of preventing postoperative leg vein thrombosis patients undergoing major surgery were divided into three groups. One received intermittent electrical calf muscle stimulation during surgery, the second subcutaneous heparin calcium 5000 IU every eight hours for six days, and the third no specific prophylaxis. Leg vein thrombosis was detected by the 125-I-fibrinogen uptake test. Neither method was effective in patients undergoing open bladder or prostatic surgery. Stimulation did not reduce the incidence of leg vein thrombosis in patients with malignant disease undergoing laparotomy, but heparin calcium was highly successful in this group (P smaller than 0-001). When the laparotomy was for a benign condition, however, both heparin calcium (P smaller than 0-001) and stimulation (P smaller than 0-01) were effective."}
{"id": "PMID:1092412", "title": "New serological test for malaria antibodies.", "content": "In an enzyme-linked immunosorbent assay test for malaria antibodies, antibodies to Plasmodium vivax and P. falciparum in man are detected using a crude antigen prepared from the simian malaria parasite P. knowlesi. The test may be suitable for epidemiological studies.", "contents": "New serological test for malaria antibodies. In an enzyme-linked immunosorbent assay test for malaria antibodies, antibodies to Plasmodium vivax and P. falciparum in man are detected using a crude antigen prepared from the simian malaria parasite P. knowlesi. The test may be suitable for epidemiological studies."}
{"id": "PMID:1092419", "title": "Dextran 70 in prophylaxis of thromboembolic disease after surgery: a clinically oriented randomized double-blind trial.", "content": "A randomized double-blind trial of prophylaxis of thromboembolism in surgical patients judged by clinical morbidity has been completed. Altogether 831 patients over 40 years of age who underwent elective surgery of the stomach, biliary system, or colon received either dextran 70 or normal saline before the operation. Thirteen of the 435 patients on saline and three of the 396 on dextran developed pulmonary embolism. Eight of these 16 patients died of pulmonary embolism--seven in the saline group and one in the dextran group. As detected either clinically or by 125I-fibrinogen scanning the incidence of deep vein thrombosis was similar in the two groups. There was no increased incidence of excessive bleeding in patients on dextran though clinical impression suggested that some patients on dextran bled excessively. This trial showed that dextran 70 administered by intravenous drip during operation is effective in preventing pulmonary embolism and, in particular, reducing mortality from this cause. It seems to be as effective as subcutaneous heparin but is easier to administer and places less of a burden on nursing services.", "contents": "Dextran 70 in prophylaxis of thromboembolic disease after surgery: a clinically oriented randomized double-blind trial. A randomized double-blind trial of prophylaxis of thromboembolism in surgical patients judged by clinical morbidity has been completed. Altogether 831 patients over 40 years of age who underwent elective surgery of the stomach, biliary system, or colon received either dextran 70 or normal saline before the operation. Thirteen of the 435 patients on saline and three of the 396 on dextran developed pulmonary embolism. Eight of these 16 patients died of pulmonary embolism--seven in the saline group and one in the dextran group. As detected either clinically or by 125I-fibrinogen scanning the incidence of deep vein thrombosis was similar in the two groups. There was no increased incidence of excessive bleeding in patients on dextran though clinical impression suggested that some patients on dextran bled excessively. This trial showed that dextran 70 administered by intravenous drip during operation is effective in preventing pulmonary embolism and, in particular, reducing mortality from this cause. It seems to be as effective as subcutaneous heparin but is easier to administer and places less of a burden on nursing services."}
{"id": "PMID:1092420", "title": "Comparison of two methods of administering bronchodilator aerosol to asthmatic patients.", "content": "In 78 patients with chronic asthma the increase in forced expiratory volume in one second (FEV-1) after the administration by intermittent positive-pressure ventilation (I.P.P.V.) of 10 mg of salbutamol was compared with that recorded after the inhalation of a conventional dose (200 mug) from a pressurized canister. The mean increase was significantly greater after I.P.P.V. administration, and the superiority of this method was greatest in patients with the lowest pretreatment FEV-1.", "contents": "Comparison of two methods of administering bronchodilator aerosol to asthmatic patients. In 78 patients with chronic asthma the increase in forced expiratory volume in one second (FEV-1) after the administration by intermittent positive-pressure ventilation (I.P.P.V.) of 10 mg of salbutamol was compared with that recorded after the inhalation of a conventional dose (200 mug) from a pressurized canister. The mean increase was significantly greater after I.P.P.V. administration, and the superiority of this method was greatest in patients with the lowest pretreatment FEV-1."}
{"id": "PMID:1092423", "title": "Treponema pallidum in nerve fibres.", "content": "Ultrathin sections of a rabbit scrotal syphiloma were examined by electron microscopy. Treponemes were observed in the endo-, peri-, and epineurium of the nerve fibre. The significance of these findings, in that infection may be transmitted via the nerve fibres and pain reduced by damage to the afferent fibres, are discussed.", "contents": "Treponema pallidum in nerve fibres. Ultrathin sections of a rabbit scrotal syphiloma were examined by electron microscopy. Treponemes were observed in the endo-, peri-, and epineurium of the nerve fibre. The significance of these findings, in that infection may be transmitted via the nerve fibres and pain reduced by damage to the afferent fibres, are discussed."}
{"id": "PMID:1092424", "title": "Pharmacokinetics of tinidazole and metronidazole in women after single large oral doses.", "content": "Serum concentrations of tinidazole and metronidazole have been measured in healthy female volunteers who received a single dose of 2 g. of each drug in a cross-over study. Bioassays against T. vaginalis showed that metronidazole achieved higher peak concentrations (mean 81 mug./ml.) than did tinidazole (mean 67 mug./ml.), while assays for unchanged drug showed higher peak concentrations of tinidazole )mean 51 mug./ml;) than of metronidazole (mean 40 mug./ml9). This discrepancy is probably due to the presence in the serum of active metabolites of metronidazole. The longer half-life of tinidazole led to significantly higher serum concentrations (by bioassay and chemical assay) of tinidazole than of metronidazole from 6 hrs onwards.", "contents": "Pharmacokinetics of tinidazole and metronidazole in women after single large oral doses. Serum concentrations of tinidazole and metronidazole have been measured in healthy female volunteers who received a single dose of 2 g. of each drug in a cross-over study. Bioassays against T. vaginalis showed that metronidazole achieved higher peak concentrations (mean 81 mug./ml.) than did tinidazole (mean 67 mug./ml.), while assays for unchanged drug showed higher peak concentrations of tinidazole )mean 51 mug./ml;) than of metronidazole (mean 40 mug./ml9). This discrepancy is probably due to the presence in the serum of active metabolites of metronidazole. The longer half-life of tinidazole led to significantly higher serum concentrations (by bioassay and chemical assay) of tinidazole than of metronidazole from 6 hrs onwards."}
{"id": "PMID:1092425", "title": "Trichomonal vaginitis. A 24-hr regimen of nimorazole compared with a 7-day regimen of metronidazole.", "content": "A 24-hr regimen of nimorazole (1 g. orally at 12-hrly intervals for three doses) was compared with metronidazole (200 mg. three times daily for 7 days) in the treatment of trichomonal vaginitis. The two treatment schedules were given to alternate patients, pregnant women being excluded. One hundred cases were treated on each schedule; roughly one-fifth of the patients in each group defaulted. There were no observed failures with either schedule. The reasons for these exceptionally good results are discussed.", "contents": "Trichomonal vaginitis. A 24-hr regimen of nimorazole compared with a 7-day regimen of metronidazole. A 24-hr regimen of nimorazole (1 g. orally at 12-hrly intervals for three doses) was compared with metronidazole (200 mg. three times daily for 7 days) in the treatment of trichomonal vaginitis. The two treatment schedules were given to alternate patients, pregnant women being excluded. One hundred cases were treated on each schedule; roughly one-fifth of the patients in each group defaulted. There were no observed failures with either schedule. The reasons for these exceptionally good results are discussed."}
{"id": "PMID:1092433", "title": "Radiological changes in controlled hypervolaemic pulmonary oedema in dogs.", "content": "Chest radiographs taken under ideal conditions and reviewed by a radiologist experienced in looking for pulmonary oedema are an accurate technique for detecting early oedema, differentiating between congestion and oedema, monitoring the increases in oedema up to the stage of alveolar flooding and in determining the effects of CPPV on an oedematous lung. However, ideal conditions do not exist in the I.C.U. Our radiologist had the advantage of a fast exposure, a normal film for comparison in the sequence and only one lesion, conditions unlikely to occur in the I.C.U. It becomes apparent that it is important to obtain the best possible image in these complicated patients. Films in the erect position, for vascular distribution, as well as fast exposures with a high MAS factor give the sharp detail required to best assess lung changes. Serial studies of the chest are a requirement of the I.C.U. patient and it is helpful if comparable films are obtained by using a fixed target-to-film distance in association with the short exposure factors. If this care is taken, chest radiographs are the most accurate non-invasive technique for detecting pulmonary oedema.", "contents": "Radiological changes in controlled hypervolaemic pulmonary oedema in dogs. Chest radiographs taken under ideal conditions and reviewed by a radiologist experienced in looking for pulmonary oedema are an accurate technique for detecting early oedema, differentiating between congestion and oedema, monitoring the increases in oedema up to the stage of alveolar flooding and in determining the effects of CPPV on an oedematous lung. However, ideal conditions do not exist in the I.C.U. Our radiologist had the advantage of a fast exposure, a normal film for comparison in the sequence and only one lesion, conditions unlikely to occur in the I.C.U. It becomes apparent that it is important to obtain the best possible image in these complicated patients. Films in the erect position, for vascular distribution, as well as fast exposures with a high MAS factor give the sharp detail required to best assess lung changes. Serial studies of the chest are a requirement of the I.C.U. patient and it is helpful if comparable films are obtained by using a fixed target-to-film distance in association with the short exposure factors. If this care is taken, chest radiographs are the most accurate non-invasive technique for detecting pulmonary oedema."}
{"id": "PMID:1092436", "title": "Coupling of glycine and alanine transport to respiration in cells of Escherichia coli.", "content": "Energy coupling for uptake of glycine and alanine in glycerol grown cells of Escherichia coli differs from that of the aromatic amino acids. Respiration and uptake of glycine and alanine show similar inactivations in cells exposed to high intensity violet light or to various concentrations of cyanide. In contrast,uptake of phenylalanine, tyrosine, and tryptophan is resistant to effects of light or cyanide. Anoxia largely inhibits uptake of glycine and alanine while that of the aromatic amino acids is only partially affected. Furthermore, ferricyanide (but not ferrocyanide) completely restores active uptake of aromatic amino acids under anoxic conditions but is without effect on glycine and alanine uptake. Adenosine 5'-triphosphate (ATP) concentration does not increase in anoxic cells exposed to ferricyanide, indicating that ATP cannot be responsible for this restoration. The data suggest that glycine and alanine represent amino acids whose transport shows a complete dependence on energy derived from respiration, while the energy for transport of the aromatic amino acids may be obtained from other sources", "contents": "Coupling of glycine and alanine transport to respiration in cells of Escherichia coli. Energy coupling for uptake of glycine and alanine in glycerol grown cells of Escherichia coli differs from that of the aromatic amino acids. Respiration and uptake of glycine and alanine show similar inactivations in cells exposed to high intensity violet light or to various concentrations of cyanide. In contrast,uptake of phenylalanine, tyrosine, and tryptophan is resistant to effects of light or cyanide. Anoxia largely inhibits uptake of glycine and alanine while that of the aromatic amino acids is only partially affected. Furthermore, ferricyanide (but not ferrocyanide) completely restores active uptake of aromatic amino acids under anoxic conditions but is without effect on glycine and alanine uptake. Adenosine 5'-triphosphate (ATP) concentration does not increase in anoxic cells exposed to ferricyanide, indicating that ATP cannot be responsible for this restoration. The data suggest that glycine and alanine represent amino acids whose transport shows a complete dependence on energy derived from respiration, while the energy for transport of the aromatic amino acids may be obtained from other sources"}
{"id": "PMID:1092437", "title": "Chemical modification of ribosomes with dimethyl sulfate: a probe to the structural organization of ribosomal proteins and RNA.", "content": "Ribosomal proteins from [14-C]dimethyl sulfate treated with 30S and 50S subunits of Escherichia coli ribosomes were separated by two-dimensional polyacrylamide gel electrophoresis and the degree of methylation of each protein was determined. Comparison of the results from this relatively non-specific chemical modification procedure with results from the milerd lysine-specific reductive alkylation procedure reported previously (Moore, G. & Crichton, R.R. (1974) Biochem. J. 143, 607-612) has permitted a topographical classification of ribosomal proteins in terms of 'degree of exposure' in the 30S and 50S subunits. The reaction of dimethyl sulfate with ribosomal RNA, both in intact subunits and after isolation from the subunits, has indicated that approximately half of the RNA in 30S and 50S subunits is exposed on the surface of the ribonucleoprotein complexes, and that no large sections (extended sequences) of 16S RNA are concealed in the 30S subunit. It is proposed that modification of ribosomes with dimethyl sulfate is a potentially useful technique for probing exposed and hidden regions and also exposed single-stranded regions of RNA in ribosomes.", "contents": "Chemical modification of ribosomes with dimethyl sulfate: a probe to the structural organization of ribosomal proteins and RNA. Ribosomal proteins from [14-C]dimethyl sulfate treated with 30S and 50S subunits of Escherichia coli ribosomes were separated by two-dimensional polyacrylamide gel electrophoresis and the degree of methylation of each protein was determined. Comparison of the results from this relatively non-specific chemical modification procedure with results from the milerd lysine-specific reductive alkylation procedure reported previously (Moore, G. & Crichton, R.R. (1974) Biochem. J. 143, 607-612) has permitted a topographical classification of ribosomal proteins in terms of 'degree of exposure' in the 30S and 50S subunits. The reaction of dimethyl sulfate with ribosomal RNA, both in intact subunits and after isolation from the subunits, has indicated that approximately half of the RNA in 30S and 50S subunits is exposed on the surface of the ribonucleoprotein complexes, and that no large sections (extended sequences) of 16S RNA are concealed in the 30S subunit. It is proposed that modification of ribosomes with dimethyl sulfate is a potentially useful technique for probing exposed and hidden regions and also exposed single-stranded regions of RNA in ribosomes."}
{"id": "PMID:1092438", "title": "Acute undifferentiated neonatal diarrhea in beef calves. I. Occurence and distribution of infectious agents.", "content": "Beef calves in a 48-cow herd were studied during one calving season from birth to ten days of age to determine the presence or absence of potentially enteropathogenic bacteria, viruses, and/or chlamydia in both normal and diarrheic calves. Calves were born and raised outside in large pens unless the ambient temperature was below minus 10 degrees F when calving was done inside. Fecal swabs, fecal aliquots, and nasal swabs were taken from each calf at 32, 128 plus or minus 3, and 248 plus or minus 3 hours of age and as soon after the onset of diarrhea as possible. Diarrhea was defined as that condition in which the feces contained less than 10% dry matter. Enteropathogenic Escherichia coli in feces were identified using the ligated gut loop procedure in calves and by feeding broth cultures to colostrum fed lambs seven to 16 hours old. Potentially enteropathogenic viruses were detected using a variety of methods which included tissue culture, fluorescent antibody, hemadsorption, and electron microscope techniques. Of the 40 calves studied, 32 (80%) developed diarrhea before ten days of age. Twenty-two strains of Escherichia coli which caused dilation of calf ligated intestinal loops were isolated from 11 scouring calves and from one normal calf. Nine out of ten strains of Escherichia coli which dilated ligated loops also caused diarrhea when fed to colostrum-fed lambs seven to 16 hours old. Using antibody technique a Reo-like virus was detected in the feces of 15 calves before, during, and after the onset of diarrhea. Four calves excreted both loop dilating strains of E. coli and Reo-like virus in the feces before ten days of age; in all cases the loop dilating E. coli were isolated from the feces prior to the demonstration of Reo-like virus. A Corona-like virus was also demonstrated in three of the 15 calves infected with Reo-like virus and a noncytopathogenic strain of bovine virus diarrhea virus was isolated from two of the 15 calves infected with Reo-like virus. A loop dilating strain of Citrobacter was isolated from one diarrheic calf. There was no consistent pattern of onset or duration of diarrhea in calves which excreted different infectious agents. Salmonella species, infectious bovine rhinotracheitis virus, parvovirus, adenoviruses, parainfluenza-3 virus, and Chlamydia species could not be demonstrated in any of the calves or their dams. No potentially enteropathogenic agents could be demonstrated in 11 of the 32 calves which scoured. These findings emphasize the complexity of the infectious aspect of the neonatal diarrhea syndrome and illustrate the difficulty in making an etiological diagnosis in field outbreaks of the calf scours complex.", "contents": "Acute undifferentiated neonatal diarrhea in beef calves. I. Occurence and distribution of infectious agents. Beef calves in a 48-cow herd were studied during one calving season from birth to ten days of age to determine the presence or absence of potentially enteropathogenic bacteria, viruses, and/or chlamydia in both normal and diarrheic calves. Calves were born and raised outside in large pens unless the ambient temperature was below minus 10 degrees F when calving was done inside. Fecal swabs, fecal aliquots, and nasal swabs were taken from each calf at 32, 128 plus or minus 3, and 248 plus or minus 3 hours of age and as soon after the onset of diarrhea as possible. Diarrhea was defined as that condition in which the feces contained less than 10% dry matter. Enteropathogenic Escherichia coli in feces were identified using the ligated gut loop procedure in calves and by feeding broth cultures to colostrum fed lambs seven to 16 hours old. Potentially enteropathogenic viruses were detected using a variety of methods which included tissue culture, fluorescent antibody, hemadsorption, and electron microscope techniques. Of the 40 calves studied, 32 (80%) developed diarrhea before ten days of age. Twenty-two strains of Escherichia coli which caused dilation of calf ligated intestinal loops were isolated from 11 scouring calves and from one normal calf. Nine out of ten strains of Escherichia coli which dilated ligated loops also caused diarrhea when fed to colostrum-fed lambs seven to 16 hours old. Using antibody technique a Reo-like virus was detected in the feces of 15 calves before, during, and after the onset of diarrhea. Four calves excreted both loop dilating strains of E. coli and Reo-like virus in the feces before ten days of age; in all cases the loop dilating E. coli were isolated from the feces prior to the demonstration of Reo-like virus. A Corona-like virus was also demonstrated in three of the 15 calves infected with Reo-like virus and a noncytopathogenic strain of bovine virus diarrhea virus was isolated from two of the 15 calves infected with Reo-like virus. A loop dilating strain of Citrobacter was isolated from one diarrheic calf. There was no consistent pattern of onset or duration of diarrhea in calves which excreted different infectious agents. Salmonella species, infectious bovine rhinotracheitis virus, parvovirus, adenoviruses, parainfluenza-3 virus, and Chlamydia species could not be demonstrated in any of the calves or their dams. No potentially enteropathogenic agents could be demonstrated in 11 of the 32 calves which scoured. These findings emphasize the complexity of the infectious aspect of the neonatal diarrhea syndrome and illustrate the difficulty in making an etiological diagnosis in field outbreaks of the calf scours complex."}
{"id": "PMID:1092439", "title": "Histochemical localization of hydrolytic enzymes during corneal graft reaction.", "content": "We studied hydrolytic enzymes in the graft bed during the corneal graft reaction. Two enzymes, beta-glucuronidase and acid phosphatase, were used as the markers of lysosomal hydrolytic enzymes. These were traced histochemically at various times before and after the onset of the graft reaction in a corneal xenograft model. Results showed a relationship between the degree of graft opacity, cellular infiltration and enzyme localization.  It appeared that during the early stages of graft reaction, the granulocytes, and during the late stages, the agranulocytes were the main source of the enzymes.", "contents": "Histochemical localization of hydrolytic enzymes during corneal graft reaction. We studied hydrolytic enzymes in the graft bed during the corneal graft reaction. Two enzymes, beta-glucuronidase and acid phosphatase, were used as the markers of lysosomal hydrolytic enzymes. These were traced histochemically at various times before and after the onset of the graft reaction in a corneal xenograft model. Results showed a relationship between the degree of graft opacity, cellular infiltration and enzyme localization.  It appeared that during the early stages of graft reaction, the granulocytes, and during the late stages, the agranulocytes were the main source of the enzymes."}
{"id": "PMID:1092440", "title": "Lethality of Candida strains as influenced by the host.", "content": "A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydospore production, germ tube formation, nor nutritional resquirements was related to pathogenicity in mice. Statistical analysis indicated that the culture media of the organisms and the strain and sex of the mice into which the cells were injected were important in the development of pathogenicity.", "contents": "Lethality of Candida strains as influenced by the host. A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydospore production, germ tube formation, nor nutritional resquirements was related to pathogenicity in mice. Statistical analysis indicated that the culture media of the organisms and the strain and sex of the mice into which the cells were injected were important in the development of pathogenicity."}
{"id": "PMID:1092441", "title": "Determination of guanine plus cytosine content in Streptococcus pyogenes.", "content": "The guanine and cytosine content of Streptococcus pyogenes DNA was determined by thermal denaturation and buoyant density analysis to be 36.7% and 38.7%, respectively.", "contents": "Determination of guanine plus cytosine content in Streptococcus pyogenes. The guanine and cytosine content of Streptococcus pyogenes DNA was determined by thermal denaturation and buoyant density analysis to be 36.7% and 38.7%, respectively."}
{"id": "PMID:1092442", "title": "Two modifications making membrane filtration more economical.", "content": "Two economical membrane filtration techniques are described: (1) vacuum filtration through a single funnel into several sterile containers and (2) pressure filtration using an inexpensive combination of pressure source, vessel, and valve.", "contents": "Two modifications making membrane filtration more economical. Two economical membrane filtration techniques are described: (1) vacuum filtration through a single funnel into several sterile containers and (2) pressure filtration using an inexpensive combination of pressure source, vessel, and valve."}
{"id": "PMID:1092443", "title": "Surgery for coronary artery disease and congestive heart failure.", "content": "A group of 41 patients presenting primarily with symptoms of congestive heart failure was investigated by coronary arteriography and myocardial revascularization. Of these patients 20 underwent resection of left ventricular aneurysm or left ventricular plication as well as revascularization. Revascularization plus resection of left ventricular aneurysm gives gratifying results, but revascularization in the presence of severe generalized hypokinesis is less satisfactory; however, the improvement in clinical condition in a sufficient number of patients is such that this approach to their management is justified. Accordingly, every patient with coronary artery disease and congestive heart failure should be investigated by angiography. Numerous factors influence the results of revascularization for the relief of congestive heart failure; the most useful as a criterion to aid patient selection is left ventriculography. In this group of 41 patients 66% were helped by myocardial revascularization.", "contents": "Surgery for coronary artery disease and congestive heart failure. A group of 41 patients presenting primarily with symptoms of congestive heart failure was investigated by coronary arteriography and myocardial revascularization. Of these patients 20 underwent resection of left ventricular aneurysm or left ventricular plication as well as revascularization. Revascularization plus resection of left ventricular aneurysm gives gratifying results, but revascularization in the presence of severe generalized hypokinesis is less satisfactory; however, the improvement in clinical condition in a sufficient number of patients is such that this approach to their management is justified. Accordingly, every patient with coronary artery disease and congestive heart failure should be investigated by angiography. Numerous factors influence the results of revascularization for the relief of congestive heart failure; the most useful as a criterion to aid patient selection is left ventriculography. In this group of 41 patients 66% were helped by myocardial revascularization."}
{"id": "PMID:1092444", "title": "Prophylactic antibiotic therapy in surgery.", "content": "Prophylactic (preventive) antibiotic therapy initiated preoperatively, with antibiotics administered in moderately high dosage for short periods, is recommended on the basis of experimental and prospective, randomized clinical trials for patients who require surgery that is likely to expose tissue planes to contamination. The value of prophylactic antibiotics in clean operations is not presently supported but must be considered in patients with decreased resistance and in those in whom infection of a prosthesis would have catastrophic results. In these patients topical antibiotics might prove useful and less dangerous. It is clear that surgical technique remains an important but as yet unmeasured factor in wound infection.", "contents": "Prophylactic antibiotic therapy in surgery. Prophylactic (preventive) antibiotic therapy initiated preoperatively, with antibiotics administered in moderately high dosage for short periods, is recommended on the basis of experimental and prospective, randomized clinical trials for patients who require surgery that is likely to expose tissue planes to contamination. The value of prophylactic antibiotics in clean operations is not presently supported but must be considered in patients with decreased resistance and in those in whom infection of a prosthesis would have catastrophic results. In these patients topical antibiotics might prove useful and less dangerous. It is clear that surgical technique remains an important but as yet unmeasured factor in wound infection."}
{"id": "PMID:1092446", "title": "Host defence mechanisms: evaluation and roles of acquired defects and immunotherapy.", "content": "The development of an infectious process is a result of the interaction of the three determinants of infection: the infecting organism, the local site of infection and systemic host defences. Interaction of the various components is important in an understanding of the development of sepsis. Acquired defects of host defence mechanisms are attributable to a variety of factors and can be both the cause and the result of sepsis. The burn injury illustrates alterations in host defence and the interaction of the determinants of sepsis. Methods of evaluating the components of host defence are available, though sometimes difficult; in the future, evaluation of the effect of one component on another will become clinically feasible, allowing more complete assessment of acquired defects. Immunotherapy is not yet widely available; however, definition of the acquired defects of host defence mechanisms in surgical patients may eventually lead to introduction of effective treatment.", "contents": "Host defence mechanisms: evaluation and roles of acquired defects and immunotherapy. The development of an infectious process is a result of the interaction of the three determinants of infection: the infecting organism, the local site of infection and systemic host defences. Interaction of the various components is important in an understanding of the development of sepsis. Acquired defects of host defence mechanisms are attributable to a variety of factors and can be both the cause and the result of sepsis. The burn injury illustrates alterations in host defence and the interaction of the determinants of sepsis. Methods of evaluating the components of host defence are available, though sometimes difficult; in the future, evaluation of the effect of one component on another will become clinically feasible, allowing more complete assessment of acquired defects. Immunotherapy is not yet widely available; however, definition of the acquired defects of host defence mechanisms in surgical patients may eventually lead to introduction of effective treatment."}
{"id": "PMID:1092447", "title": "Results of minor foot amputations for ischemia of the lower extremity in diabetics and nondiabetics.", "content": "Results of 208 minor amputations were analyzed in 179 patients who had no food pulses. Wound healing was assessed at 3 months in relation to diabetes and previous vascular surgery. The results suggest that simple removal of the toe or toes is not advisable unless the blood supply to the foot can be improved by vascular reconstruction or sympathectomy, or both. Transmetatarsal amputation should be considered more often as a conservative amputation for gangrene of the toes. The absence of a palpable posterior tibial pulse is a contraindication to the Syme's amputation.", "contents": "Results of minor foot amputations for ischemia of the lower extremity in diabetics and nondiabetics. Results of 208 minor amputations were analyzed in 179 patients who had no food pulses. Wound healing was assessed at 3 months in relation to diabetes and previous vascular surgery. The results suggest that simple removal of the toe or toes is not advisable unless the blood supply to the foot can be improved by vascular reconstruction or sympathectomy, or both. Transmetatarsal amputation should be considered more often as a conservative amputation for gangrene of the toes. The absence of a palpable posterior tibial pulse is a contraindication to the Syme's amputation."}
{"id": "PMID:1092449", "title": "The role of fomites in the transmission of vaginitis.", "content": "A role for fomites such as toilet seats in the transmission of vaginitis has never been proved or disproved. A compilation of clinical data from a university community showed that the organisms found in vaginal cultures of patients with vaginitis were, in order of frequency. Candida albicans, Escherichia coli, beta-hemolytic streptococci, Hemophilus vaginalis and Trichomonas vaginalis. In a concurrent bacteriologic survey of washroom fixtures, staphylococci and other micrococci were isolated most frequently. The overt pathogens associated with vaginitis were never found, and gram-negative organisms appeared to be suppressed by the disinfectant used by the cleaning staff. It is clear that fomites are not an important mode of transmission in vaginitis, although a search for specific pathogens on toilets is to be continued.", "contents": "The role of fomites in the transmission of vaginitis. A role for fomites such as toilet seats in the transmission of vaginitis has never been proved or disproved. A compilation of clinical data from a university community showed that the organisms found in vaginal cultures of patients with vaginitis were, in order of frequency. Candida albicans, Escherichia coli, beta-hemolytic streptococci, Hemophilus vaginalis and Trichomonas vaginalis. In a concurrent bacteriologic survey of washroom fixtures, staphylococci and other micrococci were isolated most frequently. The overt pathogens associated with vaginitis were never found, and gram-negative organisms appeared to be suppressed by the disinfectant used by the cleaning staff. It is clear that fomites are not an important mode of transmission in vaginitis, although a search for specific pathogens on toilets is to be continued."}
{"id": "PMID:1092451", "title": "Diseases associated with specific HL-A antigens.", "content": "Significantly increased prevalences of particular HL-A antigens have been reported for many human diseases. The correlation is particularly striking in ankylosing spondylitis, Reiter's syndrome, psoriasis and some immunopathic disorders, so that HL-A typing may be of great value in diagnosis. The possible mechanisms whereby these associations may occur suggest the cause of certain disorders, and further investiatation will likely help in the understanding of the pathogenesis of many diseases.", "contents": "Diseases associated with specific HL-A antigens. Significantly increased prevalences of particular HL-A antigens have been reported for many human diseases. The correlation is particularly striking in ankylosing spondylitis, Reiter's syndrome, psoriasis and some immunopathic disorders, so that HL-A typing may be of great value in diagnosis. The possible mechanisms whereby these associations may occur suggest the cause of certain disorders, and further investiatation will likely help in the understanding of the pathogenesis of many diseases."}
{"id": "PMID:1092455", "title": "Psychogenesis and somatogenesis of common symptoms.", "content": "There are situations in clinical practice in which the physican should keep in mind the influence of emotional factors in the elaboration of symptoms and yet should not conclude hastily that \"all is in the patient's mind\". Symptoms are often the result of complex etiologic factors including life-threatening illnesses presenting psychologic symptoms as an early manifestation. Psychologic disorders and physical illnesses with similar symptoms may coexist in the same patient. There are also cases in which the symptoms are the result of the constant interaction of psychologic and physical factors. Some suggestions to help to clarify the diagnosis are given and a classification of the different clinical situations involved is presented. Becuase physicians should be constantly aware of the complexity of the factors involved in the elaboration of obscure symptoms, some recommendations are given in regard to undergraduate and graduate medical education.", "contents": "Psychogenesis and somatogenesis of common symptoms. There are situations in clinical practice in which the physican should keep in mind the influence of emotional factors in the elaboration of symptoms and yet should not conclude hastily that \"all is in the patient's mind\". Symptoms are often the result of complex etiologic factors including life-threatening illnesses presenting psychologic symptoms as an early manifestation. Psychologic disorders and physical illnesses with similar symptoms may coexist in the same patient. There are also cases in which the symptoms are the result of the constant interaction of psychologic and physical factors. Some suggestions to help to clarify the diagnosis are given and a classification of the different clinical situations involved is presented. Becuase physicians should be constantly aware of the complexity of the factors involved in the elaboration of obscure symptoms, some recommendations are given in regard to undergraduate and graduate medical education."}
{"id": "PMID:1092461", "title": "Human blood-group MN and precursor specificities: structural and biological aspects.", "content": "The human blood-group MM and NN antigens carry 2 to 4 immunodominant groupings per repeating subunit and differ only by one sialic acid residue per immunodominant group. This residue covers in the MM antigen the beta-D-galactopyranosyl group that is terminal in the N immunodominant structure and that, together with a terminal alpha-linked N-acetylneuraminic acid residue, is responsible for N specificity. M specificity was readily converted into N specificity by mild acid treatment. N structure is the immediate biochemical precursor of M structure, and M and N antigenic specificities are not determined by two allelic genes as believed hitherto. The NN antigen was inactivated by beta-D-galactosidase as well as by removal of N-acetylneuraminic acid. Some of the reactivities of the NN antigen, lost upon beta-D-galactosidase treatment, reappeared on subsequent partial N-acetylneuraminic acid removal. The structure uncovered by complete sialic acid depletion of MN antigens is the Thomsen-Friedenreich T antigen, the specificity of which is determined by beta-D-galactopyranosyl groups. Beta-D-Galactosidase treatment transformed the T antigen into one possessing Tnactivity. The significance of blood-group MN active substances extends to human breast cancer, where MN antigens were found in benign and malignant glands, but some of their precursors in cancerous tissue only.", "contents": "Human blood-group MN and precursor specificities: structural and biological aspects. The human blood-group MM and NN antigens carry 2 to 4 immunodominant groupings per repeating subunit and differ only by one sialic acid residue per immunodominant group. This residue covers in the MM antigen the beta-D-galactopyranosyl group that is terminal in the N immunodominant structure and that, together with a terminal alpha-linked N-acetylneuraminic acid residue, is responsible for N specificity. M specificity was readily converted into N specificity by mild acid treatment. N structure is the immediate biochemical precursor of M structure, and M and N antigenic specificities are not determined by two allelic genes as believed hitherto. The NN antigen was inactivated by beta-D-galactosidase as well as by removal of N-acetylneuraminic acid. Some of the reactivities of the NN antigen, lost upon beta-D-galactosidase treatment, reappeared on subsequent partial N-acetylneuraminic acid removal. The structure uncovered by complete sialic acid depletion of MN antigens is the Thomsen-Friedenreich T antigen, the specificity of which is determined by beta-D-galactopyranosyl groups. Beta-D-Galactosidase treatment transformed the T antigen into one possessing Tnactivity. The significance of blood-group MN active substances extends to human breast cancer, where MN antigens were found in benign and malignant glands, but some of their precursors in cancerous tissue only."}
{"id": "PMID:1092467", "title": "Lymphocyte: erythrocyte (L.E.) rosettes as indicators of the heterogeneity of lymphocytes in a variety of mammalian species.", "content": "Lymphocytes and red cells from various mammalian species have been mixed in vitro in conditions which favor their aggregation in the form of rosettes. The frequencies of rosette formation taken in conjunction with observations on surface bound immunoglobulin on the lymphocytes favor the interpretation that, in many species of animal, rosette formation can be used as an indicator of the thymic (T) or bursal equivalent (B) origin of lymphocytes.", "contents": "Lymphocyte: erythrocyte (L.E.) rosettes as indicators of the heterogeneity of lymphocytes in a variety of mammalian species. Lymphocytes and red cells from various mammalian species have been mixed in vitro in conditions which favor their aggregation in the form of rosettes. The frequencies of rosette formation taken in conjunction with observations on surface bound immunoglobulin on the lymphocytes favor the interpretation that, in many species of animal, rosette formation can be used as an indicator of the thymic (T) or bursal equivalent (B) origin of lymphocytes."}
{"id": "PMID:1092468", "title": "The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes.", "content": "By equilibrium competition experiments, the dissociation constant (K(RD)) of lac repressor for E. coli DNA carrying a deletion of the lac operon was measured at a variety of salt concentrations. These data are used in the consideration of several aspects of protein-DNA interaction: Quantitative estimates of specificity are made. Specificity changes only slightly with salt concentration. We calculate that in vivo, 98 percent or more of repressor is bound to DNA predominately at sites other than the lac operator. Inducers shift repressor from operator to nonoperator DNA, but do not free it from DNA. The general affinity of repressor for E. coli DNA is sufficient to support a model where repressor slides along DNA for significant distances. The effective dissociation constant of repressor for operator (K(eff)) is very sensitive to the total DNA concentration. We propose that \"junk\" DNA in eucaryotes functions to maintain total DNA at an optimum concentration. We consider the lac operon in the nucleus of a lymphocyte, point out that severe difficulties would be encountered, and suggest possible solutions.", "contents": "The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes. By equilibrium competition experiments, the dissociation constant (K(RD)) of lac repressor for E. coli DNA carrying a deletion of the lac operon was measured at a variety of salt concentrations. These data are used in the consideration of several aspects of protein-DNA interaction: Quantitative estimates of specificity are made. Specificity changes only slightly with salt concentration. We calculate that in vivo, 98 percent or more of repressor is bound to DNA predominately at sites other than the lac operator. Inducers shift repressor from operator to nonoperator DNA, but do not free it from DNA. The general affinity of repressor for E. coli DNA is sufficient to support a model where repressor slides along DNA for significant distances. The effective dissociation constant of repressor for operator (K(eff)) is very sensitive to the total DNA concentration. We propose that \"junk\" DNA in eucaryotes functions to maintain total DNA at an optimum concentration. We consider the lac operon in the nucleus of a lymphocyte, point out that severe difficulties would be encountered, and suggest possible solutions."}
{"id": "PMID:1092469", "title": "Thy 1 (theta) antigen on the surface of morphologically distinct brain cell types.", "content": "Surface cultures of 15-19 day old fetal mouse brain that do not initially express the Thy 1 antigen develop the antigen between 4 and 11 days in culture. The antigen can be detected by immunofluorescence of live cells on the culture plate on two morphologically distinct types of cells. Both neuronal-looking cells and a more general category of cells which includes fibroblastic cells can express the Thy 1 antigen. The antigen is not expressed on certain cells of the glial type. This method enables one to distinguish between different types of brain cells in culture both morphologically and immunologically at the same time.", "contents": "Thy 1 (theta) antigen on the surface of morphologically distinct brain cell types. Surface cultures of 15-19 day old fetal mouse brain that do not initially express the Thy 1 antigen develop the antigen between 4 and 11 days in culture. The antigen can be detected by immunofluorescence of live cells on the culture plate on two morphologically distinct types of cells. Both neuronal-looking cells and a more general category of cells which includes fibroblastic cells can express the Thy 1 antigen. The antigen is not expressed on certain cells of the glial type. This method enables one to distinguish between different types of brain cells in culture both morphologically and immunologically at the same time."}
{"id": "PMID:1092486", "title": "Physical Factors in Denture Retention.", "content": "AAThis investigation was carried out to analyze the physical factors of saliva affecting denture retention. A model of examining denture retention is given by two parallel disks separated by a liquid layer. Metal, polyisobutylene (PIB) and poly (methyl methacrylate)(PMMA) were used instead of a denture and mucous membrane; and glycerol, olive oil and castor oil instead of saliva. The experiments were performed with three disk conditions: (1) Both upper and lower disks of metal, (2) both upper and lower disks of PMMA, (3) upper disk with PIB lining and lower of PMMA soley. A strain gauge was used in the experimental apparatus in order to obtain a measurement of high accuracy. In the experiments, the retentive forces developed in layers of 50 mu tickness were measured and compared with the values calculated from theoretical equations. The results are summarized as follows: (1)Retentive force must be resolved into static adhesive and separating forces, (2) surface tension of liquid may not highly influence the retention, and (3) viscosity of liquid plays an important role when two disks are separated.", "contents": "Physical Factors in Denture Retention. AAThis investigation was carried out to analyze the physical factors of saliva affecting denture retention. A model of examining denture retention is given by two parallel disks separated by a liquid layer. Metal, polyisobutylene (PIB) and poly (methyl methacrylate)(PMMA) were used instead of a denture and mucous membrane; and glycerol, olive oil and castor oil instead of saliva. The experiments were performed with three disk conditions: (1) Both upper and lower disks of metal, (2) both upper and lower disks of PMMA, (3) upper disk with PIB lining and lower of PMMA soley. A strain gauge was used in the experimental apparatus in order to obtain a measurement of high accuracy. In the experiments, the retentive forces developed in layers of 50 mu tickness were measured and compared with the values calculated from theoretical equations. The results are summarized as follows: (1)Retentive force must be resolved into static adhesive and separating forces, (2) surface tension of liquid may not highly influence the retention, and (3) viscosity of liquid plays an important role when two disks are separated."}
{"id": "PMID:1092500", "title": "Reticulosarcoma occurring during long-term hemodialysis.", "content": "Reticulosarcoma occuring during long-term hemodialysis. A case of chronic renal failure due to polycystic disease of the kidneys with development of a reticulosarcoma with cerebral involvement during maintenance hemodialysis is described. Immunosuppression from uremia could have played an important part in tumor induction in this case and might also be a significant factor in the high incidence of de-novo malignancies in renal transplant recipients.", "contents": "Reticulosarcoma occurring during long-term hemodialysis. Reticulosarcoma occuring during long-term hemodialysis. A case of chronic renal failure due to polycystic disease of the kidneys with development of a reticulosarcoma with cerebral involvement during maintenance hemodialysis is described. Immunosuppression from uremia could have played an important part in tumor induction in this case and might also be a significant factor in the high incidence of de-novo malignancies in renal transplant recipients."}
{"id": "PMID:1092501", "title": "Endotoxinemia in renal transplant recipients.", "content": "Endotoxinemia in renal transplant recipients. In immunosuppressed renal transplant recipients a high incidence of endotoxinemia was found during chest or urinary tract infections. Although the endotoxinemia was protracted, indicating inability to eradicate the infection, yet in general the effects were benign and adverse renal damage was not seen. The Limulus gelation test has a positive contribution to make in the diagnosis of illness in transplant recipients.", "contents": "Endotoxinemia in renal transplant recipients. Endotoxinemia in renal transplant recipients. In immunosuppressed renal transplant recipients a high incidence of endotoxinemia was found during chest or urinary tract infections. Although the endotoxinemia was protracted, indicating inability to eradicate the infection, yet in general the effects were benign and adverse renal damage was not seen. The Limulus gelation test has a positive contribution to make in the diagnosis of illness in transplant recipients."}
{"id": "PMID:1092502", "title": "A new approach to total hip replacement without osteotomy of the greater trochanter.", "content": "To be able to perform total hip replacement without osteotomy of the greater trochanter has specific advantages including reduced operating time, reduced blood loss and shorter rehabilitation time. However, the exposure is less wide than when the greater trochanter is osteotomized and likelihood of technical error is increased. A new approach is presented, involving complete capsulectomy without osteotomy of the greater trochanter, which provides wide exposure of the acetabulum and of the femoral neck. It permits total hip replacement to be done without osteotomy of the greater trochanter with satisfactory exposure in many instances. Specific contraindications against the use of this approach are those circumstances which require shortening of the limb, cases with severe distortion of the anatomy, cases of severe protrusio acetabulum and cases with marked scarring from previous surgery.", "contents": "A new approach to total hip replacement without osteotomy of the greater trochanter. To be able to perform total hip replacement without osteotomy of the greater trochanter has specific advantages including reduced operating time, reduced blood loss and shorter rehabilitation time. However, the exposure is less wide than when the greater trochanter is osteotomized and likelihood of technical error is increased. A new approach is presented, involving complete capsulectomy without osteotomy of the greater trochanter, which provides wide exposure of the acetabulum and of the femoral neck. It permits total hip replacement to be done without osteotomy of the greater trochanter with satisfactory exposure in many instances. Specific contraindications against the use of this approach are those circumstances which require shortening of the limb, cases with severe distortion of the anatomy, cases of severe protrusio acetabulum and cases with marked scarring from previous surgery."}
{"id": "PMID:1092504", "title": "The present status of the problem of pes cavus.", "content": "Notable historical aspects relating to the etiology and treatment of pes cavus have been critically examined. The characteristic features of the deformity are described and an explanation offered for the mechanism of their production. Although its etiology remains uncertain, a study of the literature and a great deal of clinical material has established certain well supported conclusions regarding the etiology and pathology of the condition. Certain aspects of cerebral palsy serve to strengthen impressions of earlier authors that the primary center of origin of pes cavus lies somewhere in the central nervous system. Localized foci of partial damage lying adjacent to tracts of nerve cells more seriously affected by a neurological disease could emit irritating stimuli capable of producing degrees of over-action of the invertor muscles varying from obvious spasm to clinically undetectable increase in muscle tone. Biral or other factors which stop short at creating nothing more than such a focus of irration could explain the insidious onset of the deformity in the idiopathic group. Over-action of invertor muscles for one reason or another, including ischemia, is almost certainly responsible for initiating the deformity, though primary contracture of the plantar fascia could possibly do so. With the appearance of supination of the heel, the calcanean tendon becomes an active invertor adding its force to that of the plantar fascia to produce structural varus of the calcaneum. Contracture of the plantar fascia and supination of the heel are regarded as features of major importance. Correction of the latter can be achieved more effectively by suitable osteotomy than by subtaloid fusion, which is regarded with great disfavor. Conservative treatment consists of exercises and shoe appliances. Surgical correction is based on calcanean osteotomy and plantar fasciotomy supplemented where necessary by suitable tendon transplantations, correction of clawing of the toes, and tarsal or metatarsal wedge resections. Preservation of the midtarsal subtaloid joint complex is essential. With the heel correctly aligned the degree of improvement to be expected in the forefoot deformity is such that any structural operation on it should be deferred until a fair period of walking has been tried.", "contents": "The present status of the problem of pes cavus. Notable historical aspects relating to the etiology and treatment of pes cavus have been critically examined. The characteristic features of the deformity are described and an explanation offered for the mechanism of their production. Although its etiology remains uncertain, a study of the literature and a great deal of clinical material has established certain well supported conclusions regarding the etiology and pathology of the condition. Certain aspects of cerebral palsy serve to strengthen impressions of earlier authors that the primary center of origin of pes cavus lies somewhere in the central nervous system. Localized foci of partial damage lying adjacent to tracts of nerve cells more seriously affected by a neurological disease could emit irritating stimuli capable of producing degrees of over-action of the invertor muscles varying from obvious spasm to clinically undetectable increase in muscle tone. Biral or other factors which stop short at creating nothing more than such a focus of irration could explain the insidious onset of the deformity in the idiopathic group. Over-action of invertor muscles for one reason or another, including ischemia, is almost certainly responsible for initiating the deformity, though primary contracture of the plantar fascia could possibly do so. With the appearance of supination of the heel, the calcanean tendon becomes an active invertor adding its force to that of the plantar fascia to produce structural varus of the calcaneum. Contracture of the plantar fascia and supination of the heel are regarded as features of major importance. Correction of the latter can be achieved more effectively by suitable osteotomy than by subtaloid fusion, which is regarded with great disfavor. Conservative treatment consists of exercises and shoe appliances. Surgical correction is based on calcanean osteotomy and plantar fasciotomy supplemented where necessary by suitable tendon transplantations, correction of clawing of the toes, and tarsal or metatarsal wedge resections. Preservation of the midtarsal subtaloid joint complex is essential. With the heel correctly aligned the degree of improvement to be expected in the forefoot deformity is such that any structural operation on it should be deferred until a fair period of walking has been tried."}
{"id": "PMID:1092505", "title": "Fracture treatment. The still unsolved problem.", "content": "The problem of the best method for fracture treatment is unsolved and awaits in genuity and intensive research. It is not known whether it is better to treat a fracture by rigid immobilization or by cyclical loading systems. Reports on the treatment of healing bones by the various contemporary methods are contradictory and inconclusive owing to the inherent lack of control in clinical studies. Selected experimental studies on the various biomechanical factors in fracture healing suggest that mechanical variables definitely alter qualitatively the healing of fractured bone. Although no studies have been specifically designed to show which mechanical factors resulted in the most efficient fracture healing, investigations suggest that healing under conditions of rigid fixation results in qualitative structural differences which constitute a weaker bone.", "contents": "Fracture treatment. The still unsolved problem. The problem of the best method for fracture treatment is unsolved and awaits in genuity and intensive research. It is not known whether it is better to treat a fracture by rigid immobilization or by cyclical loading systems. Reports on the treatment of healing bones by the various contemporary methods are contradictory and inconclusive owing to the inherent lack of control in clinical studies. Selected experimental studies on the various biomechanical factors in fracture healing suggest that mechanical variables definitely alter qualitatively the healing of fractured bone. Although no studies have been specifically designed to show which mechanical factors resulted in the most efficient fracture healing, investigations suggest that healing under conditions of rigid fixation results in qualitative structural differences which constitute a weaker bone."}
{"id": "PMID:1092506", "title": "The orthopaedic research society. Its first twenty years.", "content": "The Orthopaedic Research Society represents one of the many organizations which has contributed to advances necessary to establish orthopedics as a distinct specialty. The foresight and dedication of its founders and leaders undoubtedly stimulated vital musculoskeletal research upon which much of our present day therapy is based. The prodigious growth of the society in a brief twenty years in number of members, papers presented, and, most importantly, in quality of research engendered, reflects the vitality of contemporary orthopedics. Undoubtedly the Society will contunue to serve as a forum where investigators, both experienced and beginner, may present results of their latest attempts to solve the all too many mysteries of the musculoskeletal system.", "contents": "The orthopaedic research society. Its first twenty years. The Orthopaedic Research Society represents one of the many organizations which has contributed to advances necessary to establish orthopedics as a distinct specialty. The foresight and dedication of its founders and leaders undoubtedly stimulated vital musculoskeletal research upon which much of our present day therapy is based. The prodigious growth of the society in a brief twenty years in number of members, papers presented, and, most importantly, in quality of research engendered, reflects the vitality of contemporary orthopedics. Undoubtedly the Society will contunue to serve as a forum where investigators, both experienced and beginner, may present results of their latest attempts to solve the all too many mysteries of the musculoskeletal system."}
{"id": "PMID:1092507", "title": "Synovectomy of the hip in juvenile rheumatoid arthritis.", "content": "Review of the results of subtotal and complete synovectomy of 9 hips in 5 female patients with subluxation or progressive joint destruction (roentgenographically) than any 2 years showed improvement or retention of joint motion in 4 patients. Complete synovectomy, performed in 7 hips, necessitated surgical dislocation of the joint, but there was no evidence that this surgical dislocation comprised the proximal femoral blood supply. The mildest synovial and cartilagenous changes were present in the patient who has bilateral partial synovectomies. At follow-up these hips showed more severe destruction evaluated during a minimum of the hips treated by total synovectomy. No postoperative dislocations occurred. Synovectomy of the hip may be useful for progressive hip involvement in younger patients whose skeletal immaturity contraindicates major reconstructive procedures.", "contents": "Synovectomy of the hip in juvenile rheumatoid arthritis. Review of the results of subtotal and complete synovectomy of 9 hips in 5 female patients with subluxation or progressive joint destruction (roentgenographically) than any 2 years showed improvement or retention of joint motion in 4 patients. Complete synovectomy, performed in 7 hips, necessitated surgical dislocation of the joint, but there was no evidence that this surgical dislocation comprised the proximal femoral blood supply. The mildest synovial and cartilagenous changes were present in the patient who has bilateral partial synovectomies. At follow-up these hips showed more severe destruction evaluated during a minimum of the hips treated by total synovectomy. No postoperative dislocations occurred. Synovectomy of the hip may be useful for progressive hip involvement in younger patients whose skeletal immaturity contraindicates major reconstructive procedures."}
{"id": "PMID:1092510", "title": "Bacteremia in pediatric patients following dental manipulations.", "content": "A recent study has claimed that children who do not have gingivitis do not have bacteremia following dental manipulations and that these children do not require antibiotic prophylaxis. Postoperative blood cultures from 53 children and adolescents with and without gingivitis drawn within ten minutes of elevation of the gums, extraction of teeth, and extensive gingivectomies were sterile in all cases. The collection and laboratory methods and media were carefully evaluated and found satisfactory. However, the time interval between the dental procedure and the collection of the blood is believed to have been too short for reliable results and that the abandonment of the antibiotic prophylaxis in children is unwarranted.", "contents": "Bacteremia in pediatric patients following dental manipulations. A recent study has claimed that children who do not have gingivitis do not have bacteremia following dental manipulations and that these children do not require antibiotic prophylaxis. Postoperative blood cultures from 53 children and adolescents with and without gingivitis drawn within ten minutes of elevation of the gums, extraction of teeth, and extensive gingivectomies were sterile in all cases. The collection and laboratory methods and media were carefully evaluated and found satisfactory. However, the time interval between the dental procedure and the collection of the blood is believed to have been too short for reliable results and that the abandonment of the antibiotic prophylaxis in children is unwarranted."}
{"id": "PMID:1092508", "title": "Intertrochanteric osteotomy for degenerative hip disease. Indications.", "content": "A retrospective study was carried out to determine what were the features of either success or failure of intertrochanteric osteotomy of the hip for degenerative hip disease. Seventy-three patients (78 hips) were reviewed clinically and roentgenographically with a follow-up of two to 12 years. The hips were classified into the superior, medial and intermediate types of the primary degenerative hip disease and into the dysplastic and coxa magna variety for the secondary degenerative hip disease and then further classified into Stage I and Stage II. Stage I was defined by the roentgenographic criteria common to most of the successful procedures (78%) and Stage II was defined by criteria common to the failures (77%). Each of the stages was defined for the various types of degenerative hip disease. The roentgenographic criteria for Stage I are suggested as prerequisitive for indicating intertrochanteric osteotomy for treatment of degenerative hip disease. The roentgenographic criteria for Stage II are suggested as an indication for an operative procedure other than osteotomy. The postoperative course in the successful case characteristically showed an immediate pain relief and gradual improvement in walking, motion and function over a period of at least 6 years.", "contents": "Intertrochanteric osteotomy for degenerative hip disease. Indications. A retrospective study was carried out to determine what were the features of either success or failure of intertrochanteric osteotomy of the hip for degenerative hip disease. Seventy-three patients (78 hips) were reviewed clinically and roentgenographically with a follow-up of two to 12 years. The hips were classified into the superior, medial and intermediate types of the primary degenerative hip disease and into the dysplastic and coxa magna variety for the secondary degenerative hip disease and then further classified into Stage I and Stage II. Stage I was defined by the roentgenographic criteria common to most of the successful procedures (78%) and Stage II was defined by criteria common to the failures (77%). Each of the stages was defined for the various types of degenerative hip disease. The roentgenographic criteria for Stage I are suggested as prerequisitive for indicating intertrochanteric osteotomy for treatment of degenerative hip disease. The roentgenographic criteria for Stage II are suggested as an indication for an operative procedure other than osteotomy. The postoperative course in the successful case characteristically showed an immediate pain relief and gradual improvement in walking, motion and function over a period of at least 6 years."}
{"id": "PMID:1092511", "title": "Effect of tricyclic antidepressants and alcohol in psychomotor skills related to driving.", "content": "Twenty healthy subjects took amitriptyline, doxepin, and placebo for 2 wk each in a double-blind crossover trial, and another 20 subjects similarly took nortriptyline, chlorimipramine, and placebo. The antidepressants were given three times daily in doses generally used for neurotic patients. The presence of antidepressants in tissues was checked with the tyramine pressor test. On the seventh and fourteenth days of each period, psychomotor skills (choice reaction, coordination, and attention) were measured after the administration of drugs in combination with an alcoholic or placebo drink. Dose-response graphs for the tyramine pressor effect were shifted to the right during the antidepressant treatment, indicating a blockade of the membrane pump in peripheral sympathetic terminals. This antityramine effect of antidepressants did not correlate with their psychomotor effects. No drug alone importantly impaired psychomotor skills. Amitriptyline in combination with alcohol increased cumulative choice reaction times, and doxepin in combination with alcohol increased both cumulative choice reaction times and inaccuracy of reactions. Coordination was impaired after both of these combinations on the seventh day. It seems as if doxepin and amitriptyline but not nortiriptyline or chlorimipramine, in combination with 0.5 gm/kg of alcohol, may be especially dangerous in driving.", "contents": "Effect of tricyclic antidepressants and alcohol in psychomotor skills related to driving. Twenty healthy subjects took amitriptyline, doxepin, and placebo for 2 wk each in a double-blind crossover trial, and another 20 subjects similarly took nortriptyline, chlorimipramine, and placebo. The antidepressants were given three times daily in doses generally used for neurotic patients. The presence of antidepressants in tissues was checked with the tyramine pressor test. On the seventh and fourteenth days of each period, psychomotor skills (choice reaction, coordination, and attention) were measured after the administration of drugs in combination with an alcoholic or placebo drink. Dose-response graphs for the tyramine pressor effect were shifted to the right during the antidepressant treatment, indicating a blockade of the membrane pump in peripheral sympathetic terminals. This antityramine effect of antidepressants did not correlate with their psychomotor effects. No drug alone importantly impaired psychomotor skills. Amitriptyline in combination with alcohol increased cumulative choice reaction times, and doxepin in combination with alcohol increased both cumulative choice reaction times and inaccuracy of reactions. Coordination was impaired after both of these combinations on the seventh day. It seems as if doxepin and amitriptyline but not nortiriptyline or chlorimipramine, in combination with 0.5 gm/kg of alcohol, may be especially dangerous in driving."}
{"id": "PMID:1092512", "title": "Comparative respiratory depression of tillidine and morphine.", "content": "Respiratory depression induced by tilidine was compared with that of morphine in a crossover study in 6 healthy subjects. Increments of tilidine, 150 mg/70 kg, and morphine, 10 mg/70 kg, were given intravenously and displacement of each subject's CO2 response curve was measured after each dose increment. Both tilidine and morphine caused dose-related displacement of the CO2 response curves to the right. Approximately 80 to 120 mg of tilidine can be expected to induce the respiratory depression of morphine, 10 mg, when given intravenously. Subjective effects after tilidine were qualitatively similar to those of morphine but were of longer duration with nausea and vomiting more frequent. The respiratory depression of both drugs was effectively antagonized by naloxone.", "contents": "Comparative respiratory depression of tillidine and morphine. Respiratory depression induced by tilidine was compared with that of morphine in a crossover study in 6 healthy subjects. Increments of tilidine, 150 mg/70 kg, and morphine, 10 mg/70 kg, were given intravenously and displacement of each subject's CO2 response curve was measured after each dose increment. Both tilidine and morphine caused dose-related displacement of the CO2 response curves to the right. Approximately 80 to 120 mg of tilidine can be expected to induce the respiratory depression of morphine, 10 mg, when given intravenously. Subjective effects after tilidine were qualitatively similar to those of morphine but were of longer duration with nausea and vomiting more frequent. The respiratory depression of both drugs was effectively antagonized by naloxone."}
{"id": "PMID:1092509", "title": "The pathology of Legg-Perthes disease and its comparison with aseptic necrosis.", "content": "A review of pathological material obtained in cases of Legg-Perthes disease and in aseptic necrosis in the adult shows some similarities in distribution of the involved segment of the femoral head. X-ray signs of separation of the central avascular segment from the superior lateral and medial viable segments of the femoral head are recognizable. Crushing in of the weight-bearing segment then occurs and is recognized by flattening of the femoral head outline. At this point the child is in danger of suffering lateral displacement of the viable lateral segment leading to an incongruous extruded femoral head in relation to the acetabulum. Treatment measures must be taken prior to the occurrence of this lateral epiphyseal fracture in order to prevent the development of the stage of the disease where the prognosis is poor.", "contents": "The pathology of Legg-Perthes disease and its comparison with aseptic necrosis. A review of pathological material obtained in cases of Legg-Perthes disease and in aseptic necrosis in the adult shows some similarities in distribution of the involved segment of the femoral head. X-ray signs of separation of the central avascular segment from the superior lateral and medial viable segments of the femoral head are recognizable. Crushing in of the weight-bearing segment then occurs and is recognized by flattening of the femoral head outline. At this point the child is in danger of suffering lateral displacement of the viable lateral segment leading to an incongruous extruded femoral head in relation to the acetabulum. Treatment measures must be taken prior to the occurrence of this lateral epiphyseal fracture in order to prevent the development of the stage of the disease where the prognosis is poor."}
{"id": "PMID:1092513", "title": "Deanol and methylphenidate in minimal brain dysfunction.", "content": "Deanol, a putative acetylcholine precursor, has been used as a treatment for childhood hyperactivity for years. Efficacy has not been satisfactorily established, however. Seventy-four children referred for problems with learning, including many with hyperactivity, were screened for neurological or psychiatric illness, then given deanol, methylphenidate, or placebo in a double-blind fashion for 3 months. Maintenance dose for methylphenidate was 40 mg daily; for deanol, 500 mg. Behavior rating forms, reaction time, and a series of standard psychometric tests were given before and after treatment. Both drugs showed significant improvement on a number of tests; the pattern and degree of change differed slightly for the two. In this paradigm, deanol thus appeared to improve performance in children with learning and behavior disorders. The mechanism of action remains speculative; proof that deanol increases acetylcholine is scanty, and there is a theoretical basis for actually assuming an anticholinergic effect. Further clinical studies on deanol are indicated.", "contents": "Deanol and methylphenidate in minimal brain dysfunction. Deanol, a putative acetylcholine precursor, has been used as a treatment for childhood hyperactivity for years. Efficacy has not been satisfactorily established, however. Seventy-four children referred for problems with learning, including many with hyperactivity, were screened for neurological or psychiatric illness, then given deanol, methylphenidate, or placebo in a double-blind fashion for 3 months. Maintenance dose for methylphenidate was 40 mg daily; for deanol, 500 mg. Behavior rating forms, reaction time, and a series of standard psychometric tests were given before and after treatment. Both drugs showed significant improvement on a number of tests; the pattern and degree of change differed slightly for the two. In this paradigm, deanol thus appeared to improve performance in children with learning and behavior disorders. The mechanism of action remains speculative; proof that deanol increases acetylcholine is scanty, and there is a theoretical basis for actually assuming an anticholinergic effect. Further clinical studies on deanol are indicated."}
{"id": "PMID:1092514", "title": "Interaction of ephedrine and theophylline.", "content": "The effectiveness and safety of individualized theophylline doses administered as such and in combination with ephedrine and hydroxyzine was investigated in 23 children with chronic asthma who received these medications and a placebo for 1 week each, double-blind and in randomized sequence. Theophylline, determined by prior clinical titration in each patient, averaged 7.3 mg/kg per dose administered every 6 hr, with peak serum theophylline concentrations averaging about 16 mug/ml. Asthmatic symptoms were controlled with theophylline. Ephedrine, alone or in combination, was relatively ineffectual in these patients. None of the drugs given alone was associated with significant adverse effects but both ephedrine-theophylline combinations (with and without hydroxyzine) were associated with insomnia, nervousness, and gastrointestinal complaints. Since the combinations were not significantly more effective than theophylline alone, the apparent toxicity of these combinations suggests a contraindication for the routine use of ephedrine with theophylline in the management of chronic asthma.", "contents": "Interaction of ephedrine and theophylline. The effectiveness and safety of individualized theophylline doses administered as such and in combination with ephedrine and hydroxyzine was investigated in 23 children with chronic asthma who received these medications and a placebo for 1 week each, double-blind and in randomized sequence. Theophylline, determined by prior clinical titration in each patient, averaged 7.3 mg/kg per dose administered every 6 hr, with peak serum theophylline concentrations averaging about 16 mug/ml. Asthmatic symptoms were controlled with theophylline. Ephedrine, alone or in combination, was relatively ineffectual in these patients. None of the drugs given alone was associated with significant adverse effects but both ephedrine-theophylline combinations (with and without hydroxyzine) were associated with insomnia, nervousness, and gastrointestinal complaints. Since the combinations were not significantly more effective than theophylline alone, the apparent toxicity of these combinations suggests a contraindication for the routine use of ephedrine with theophylline in the management of chronic asthma."}
{"id": "PMID:1092515", "title": "Controlled trial of propranolol in intermittent claudication.", "content": "Seven patients (5 with arteriosclerosis obliterans and 2 with Buerger's disease) completed a two-phase double-blind crossover trial of propranolol in intermittent claudication. Performance was measured on a moving treadmill. In the initial phase, the patients were hospitalized in order to determine an \"effective\" dose of propranolol. Improvement was noted in all: after 1,600 mg in 5 and after 240 mg and 600 mg in the others. The controlled phase was carried out on an outpatient basis over 8 weeks, the patients receiving propranolol and placebo in a random manner, each for two 2-week periods. Comparison of matched periods of drug and placebo revealed no advantage for propranolol. Patients' performances deteriorated with time. None of the patients evidenced deterioration of occlusive peripheral arterial disease that could be attributed to propranolol, in spite of the high doses used.", "contents": "Controlled trial of propranolol in intermittent claudication. Seven patients (5 with arteriosclerosis obliterans and 2 with Buerger's disease) completed a two-phase double-blind crossover trial of propranolol in intermittent claudication. Performance was measured on a moving treadmill. In the initial phase, the patients were hospitalized in order to determine an \"effective\" dose of propranolol. Improvement was noted in all: after 1,600 mg in 5 and after 240 mg and 600 mg in the others. The controlled phase was carried out on an outpatient basis over 8 weeks, the patients receiving propranolol and placebo in a random manner, each for two 2-week periods. Comparison of matched periods of drug and placebo revealed no advantage for propranolol. Patients' performances deteriorated with time. None of the patients evidenced deterioration of occlusive peripheral arterial disease that could be attributed to propranolol, in spite of the high doses used."}
{"id": "PMID:1092516", "title": "Fenfluramine in man: hypophagia associated with diminished serotonin turnover.", "content": "A double-blind trial of orally administered fenfluramine was conducted in 7 non-obese adults with various neurological disorders. Caloric intake and body weight fell significantly after 8 days of treatment although there was no definite change in appetite ratings. Average central turnover of serotonin, as estimated by the cerebrospinal fluid (CSF) accumulation of 5-hydroxyindoleacetic acid (5-HIAA) during probenecid loading, decreased by 66%. No significant change in homovanillic acid, the major dopamine product, was apparent. The results support the contention that the effect of fenfluramine on human dietary intake may be mediated by alterations in serotonergic rather than dopaminergic mechanisms.", "contents": "Fenfluramine in man: hypophagia associated with diminished serotonin turnover. A double-blind trial of orally administered fenfluramine was conducted in 7 non-obese adults with various neurological disorders. Caloric intake and body weight fell significantly after 8 days of treatment although there was no definite change in appetite ratings. Average central turnover of serotonin, as estimated by the cerebrospinal fluid (CSF) accumulation of 5-hydroxyindoleacetic acid (5-HIAA) during probenecid loading, decreased by 66%. No significant change in homovanillic acid, the major dopamine product, was apparent. The results support the contention that the effect of fenfluramine on human dietary intake may be mediated by alterations in serotonergic rather than dopaminergic mechanisms."}
{"id": "PMID:1092517", "title": "Lack of effect of rheumatoid arthritis on clonixin metabolism.", "content": "The effect of rheumatoid arthritis on the metabolism of the analgesic, 2-(3-chloro-o-toluidino) nicotinic acid (clonixin), was evaluated in 12 patients with rheumatoid arthritis and in 12 matched healthy control subjects. Males, age-matched by decades, had no renal, gastrointestinal, or hepatic disease, and took no drugs during the study. Lower (p less than 0.02) serum albumins and higher globulins in the patients (albumin: 3.87 plus or minus 0.18; globulin: 3.14 plus or minus 0.28 gm/100 ml) than in the control subjects (albumin: 4.42 plus or minus 0.10; globulin: 2.36 plus or minus 0.08 gm/100 ml) were considered to be manifestations of rheumatoid arthritis. Fasting subjects were given single oral doses of 750 mg of clonixin. A spectrophotometric method was used to determine drug blood levels. Serum half-life was 1.45 plus or minus 0.12 hr in patients and 1.50 plus or minus 0.13 hr in control subjects (p greater than 0.5). Mean peak concentration developed at 1.7 hr and was 40.0 plus or minus 2.6 mug/ml for patients and 46.1 plus or minus 3.1 mug/ml for control subjects. Thus a single oral dose of clonixin results in comparable blood levels in male patients suffering from rheumatoid arthritis and in healthy control subjects.", "contents": "Lack of effect of rheumatoid arthritis on clonixin metabolism. The effect of rheumatoid arthritis on the metabolism of the analgesic, 2-(3-chloro-o-toluidino) nicotinic acid (clonixin), was evaluated in 12 patients with rheumatoid arthritis and in 12 matched healthy control subjects. Males, age-matched by decades, had no renal, gastrointestinal, or hepatic disease, and took no drugs during the study. Lower (p less than 0.02) serum albumins and higher globulins in the patients (albumin: 3.87 plus or minus 0.18; globulin: 3.14 plus or minus 0.28 gm/100 ml) than in the control subjects (albumin: 4.42 plus or minus 0.10; globulin: 2.36 plus or minus 0.08 gm/100 ml) were considered to be manifestations of rheumatoid arthritis. Fasting subjects were given single oral doses of 750 mg of clonixin. A spectrophotometric method was used to determine drug blood levels. Serum half-life was 1.45 plus or minus 0.12 hr in patients and 1.50 plus or minus 0.13 hr in control subjects (p greater than 0.5). Mean peak concentration developed at 1.7 hr and was 40.0 plus or minus 2.6 mug/ml for patients and 46.1 plus or minus 3.1 mug/ml for control subjects. Thus a single oral dose of clonixin results in comparable blood levels in male patients suffering from rheumatoid arthritis and in healthy control subjects."}
{"id": "PMID:1092518", "title": "Variation in the proportions of individual pepsins secreted by the cat in response to vagal stimulation and hypoglycaemia.", "content": "1. Gastric juice was collected at regular intervals during electrical stimulation of the vagus in anaesthetized cats and during insulin hypoglycaemia in both anaesthetized and conscious cats. The total amounts of acid and pepsin secreted were similar in the three groups. 2. Pepsins were examined by agar-gel electrophoresis. Resting juice contained two pepsins, and up to nine pepsins could be detected after stimulation. Three patterns of pepsin secretion were found. 3. The most noticeable feature was the variation in the proportion of total pepsin attributable to the pepsin which migrated most rapidly during electrophoresis (pepsin 1). In response to insulin hypoglycaemia, anaesthetized cats secreted only a small proportion of total pepsin 1 and conscious cats secreted a large proportion as pepsin 1. During direct electrical stimulation of the vagus, the proportion of pepsin 1 rose. 4. The possibility of a dependence of pepsin 1 secretion on vagal stimulation is discussed and the relevance of this to peptic ulcer and to vagotomy is considered.", "contents": "Variation in the proportions of individual pepsins secreted by the cat in response to vagal stimulation and hypoglycaemia. 1. Gastric juice was collected at regular intervals during electrical stimulation of the vagus in anaesthetized cats and during insulin hypoglycaemia in both anaesthetized and conscious cats. The total amounts of acid and pepsin secreted were similar in the three groups. 2. Pepsins were examined by agar-gel electrophoresis. Resting juice contained two pepsins, and up to nine pepsins could be detected after stimulation. Three patterns of pepsin secretion were found. 3. The most noticeable feature was the variation in the proportion of total pepsin attributable to the pepsin which migrated most rapidly during electrophoresis (pepsin 1). In response to insulin hypoglycaemia, anaesthetized cats secreted only a small proportion of total pepsin 1 and conscious cats secreted a large proportion as pepsin 1. During direct electrical stimulation of the vagus, the proportion of pepsin 1 rose. 4. The possibility of a dependence of pepsin 1 secretion on vagal stimulation is discussed and the relevance of this to peptic ulcer and to vagotomy is considered."}
{"id": "PMID:1092519", "title": "Permanent hypertension after renal homotransplantation in man.", "content": "1. In forty-one patients who underwent renal homotransplantation the following measurements were made: (a) blood flow and its distribution in the transplanted kidney as measured by the 85-Kr washout method; (b) renin release in the renal vein of the transplant; (c) arteriovenous difference in plasma renin activity (PRA) of the recipient's remaining left kidney. 2. Eleven transplanted patients were normotensive. Renal haemodynamic data were comparable with those obtained in potential kidney donors. 3. Three hypertensive patients had chronic rejection. The mean renal blood flow and the percentage flow in the first component of the washout curve were reduced. Renin release from the transplant, however, was normal. 4. Ten hypertensive patients had transplant artery stenosis. In eight of them renin release from the grafts as well as peripheral PRA were within normal range. This result is similar to experimental data obtained in Goldblatt renovascular hypertension. The two patients with the tightest artery stenosis had an elevated renin release from the transplant. 5. Thirteen hypertensive patients had elevated arteriovenous difference in PRA of the recipient's own left kidney. Peripheral PRA was significantly higher than in normotensive patients. Left nephrectomy relieved hypertension in ten of them; three have not so far undergone nephrectomy. 6. In four other cases hypertension was also relieved by removal of the patient's own kidney; however, the arteriovenous difference in PRA of that kidney fell within normal range.", "contents": "Permanent hypertension after renal homotransplantation in man. 1. In forty-one patients who underwent renal homotransplantation the following measurements were made: (a) blood flow and its distribution in the transplanted kidney as measured by the 85-Kr washout method; (b) renin release in the renal vein of the transplant; (c) arteriovenous difference in plasma renin activity (PRA) of the recipient's remaining left kidney. 2. Eleven transplanted patients were normotensive. Renal haemodynamic data were comparable with those obtained in potential kidney donors. 3. Three hypertensive patients had chronic rejection. The mean renal blood flow and the percentage flow in the first component of the washout curve were reduced. Renin release from the transplant, however, was normal. 4. Ten hypertensive patients had transplant artery stenosis. In eight of them renin release from the grafts as well as peripheral PRA were within normal range. This result is similar to experimental data obtained in Goldblatt renovascular hypertension. The two patients with the tightest artery stenosis had an elevated renin release from the transplant. 5. Thirteen hypertensive patients had elevated arteriovenous difference in PRA of the recipient's own left kidney. Peripheral PRA was significantly higher than in normotensive patients. Left nephrectomy relieved hypertension in ten of them; three have not so far undergone nephrectomy. 6. In four other cases hypertension was also relieved by removal of the patient's own kidney; however, the arteriovenous difference in PRA of that kidney fell within normal range."}
{"id": "PMID:1092525", "title": "The fluorescent treponemal antibody-absorption (FTA-ABS) test for syphilis.", "content": "The FTA test was developed at a time when immunofluorescence procedures were not well-defined. Through technique control and research, a modification of the FTA test, the FTA-ABS, has attained a position as one of the leading treponemal tests to confirm the reagin tests for syphilis. In this review of the FTA-ABS test, attention has been focused on reagent development, with the anticipation that reagent standardization may soon become a reality. The T. pallidum antigen obtained by extracting infected rabbit testicular tissue has evolved from a preparation in which the treponemes remained in the initial extracting fluid to a reagent that can be free of rabbit tissue and globulin. These washed antigen preparations improve visibility of the treponemes on the microscope slide, reduce background fluorescence, and reduce or prevent from occurring nonspecific reactions that are a result of tissue and globulin components. Both washed and nonwashed antigens are available commercially, and, to date, little differentiation has appeared on the product label. The predominant immunoglobulin that reacts with T. pallidum in the indirect fluorescent antibody tests appears to be IgG. This is the major immunoglobulin detected in the FTA-ABS test. IgM, although increased in early syphilis, is also increased in other clinical conditions. Several reports suggest that adult IgM detection in the present FTA-ABS test would be nonspecific. Until specific IgM antibody in adult syphilis can be detected without a risk to test specificity, the conjugate for the FTA-ABS test should continue to be an anti-IgG reagent. Class-specific, anti-IgG reagents are more expensive than other reagents; however, their use may eliminate the problem of nonspecificity resulting from IgM detection. Additionally, micromethods can be used to reduce cost, and this possibility should be investigated. The sorbent that contains an antigen to the Reiter treponeme may or may not specifically absorb the reactivity that occurs in normal sera; certainly, there are questionable aspects about this reagent. Group antibodies not related to Reiter treponemes may be responsible for some nonspecific reactivity; additionally, antiglobulin factors have been reported to participate in the reaction. Antigens free of rabbit serum factors and class-specific, antiimmunoglobulin reagents are available, and may lead to a better understanding of nonspecific reactions. These reagents should allow resolution of the possible multiplicity of reactivity. In this interim period, the sorbent, with its possible nonspecific nature, appears to maintain a biological balance between natural or group and immune antibodies when used to detect IgG antibody.", "contents": "The fluorescent treponemal antibody-absorption (FTA-ABS) test for syphilis. The FTA test was developed at a time when immunofluorescence procedures were not well-defined. Through technique control and research, a modification of the FTA test, the FTA-ABS, has attained a position as one of the leading treponemal tests to confirm the reagin tests for syphilis. In this review of the FTA-ABS test, attention has been focused on reagent development, with the anticipation that reagent standardization may soon become a reality. The T. pallidum antigen obtained by extracting infected rabbit testicular tissue has evolved from a preparation in which the treponemes remained in the initial extracting fluid to a reagent that can be free of rabbit tissue and globulin. These washed antigen preparations improve visibility of the treponemes on the microscope slide, reduce background fluorescence, and reduce or prevent from occurring nonspecific reactions that are a result of tissue and globulin components. Both washed and nonwashed antigens are available commercially, and, to date, little differentiation has appeared on the product label. The predominant immunoglobulin that reacts with T. pallidum in the indirect fluorescent antibody tests appears to be IgG. This is the major immunoglobulin detected in the FTA-ABS test. IgM, although increased in early syphilis, is also increased in other clinical conditions. Several reports suggest that adult IgM detection in the present FTA-ABS test would be nonspecific. Until specific IgM antibody in adult syphilis can be detected without a risk to test specificity, the conjugate for the FTA-ABS test should continue to be an anti-IgG reagent. Class-specific, anti-IgG reagents are more expensive than other reagents; however, their use may eliminate the problem of nonspecificity resulting from IgM detection. Additionally, micromethods can be used to reduce cost, and this possibility should be investigated. The sorbent that contains an antigen to the Reiter treponeme may or may not specifically absorb the reactivity that occurs in normal sera; certainly, there are questionable aspects about this reagent. Group antibodies not related to Reiter treponemes may be responsible for some nonspecific reactivity; additionally, antiglobulin factors have been reported to participate in the reaction. Antigens free of rabbit serum factors and class-specific, antiimmunoglobulin reagents are available, and may lead to a better understanding of nonspecific reactions. These reagents should allow resolution of the possible multiplicity of reactivity. In this interim period, the sorbent, with its possible nonspecific nature, appears to maintain a biological balance between natural or group and immune antibodies when used to detect IgG antibody."}
{"id": "PMID:1092531", "title": "Response of patients with chronic obstructive lung disease to the regular administration of nebulized isoproterenol. A double-blind crossover study.", "content": "The effect of the regular use of neublized isoproterenol in 14 patients with symptomatic chronic obstructive lung disease (COLD) was evaluated in a double-blind crossover 16-week study. FEV1, FVC and SGaw were measured before and 45 minutes after bronchodilator therapy every two weeks, while arterial blood gases were measured every eight weeks, before and 45 minutes after bronchodilator therapy. When the patients were considered as a group, there was no significant difference in mean symptom scores or objective pulmonary functions during the drug and placebo periods. Four patients had significantly higher (p less than .05) and two patients significantly lower mean values for at least one of the pulmonary function tests during the isoproterenol period. The patient who is most likely to benefit from isoproterenol on a regular basis appears to have the following characteristics; (1) consistent improvement in pulmonary function tests 45 minutes after use of nebulized bronchodilator; (2) moderate rather than severe COLD; and (3) a relatively normal DLCO.", "contents": "Response of patients with chronic obstructive lung disease to the regular administration of nebulized isoproterenol. A double-blind crossover study. The effect of the regular use of neublized isoproterenol in 14 patients with symptomatic chronic obstructive lung disease (COLD) was evaluated in a double-blind crossover 16-week study. FEV1, FVC and SGaw were measured before and 45 minutes after bronchodilator therapy every two weeks, while arterial blood gases were measured every eight weeks, before and 45 minutes after bronchodilator therapy. When the patients were considered as a group, there was no significant difference in mean symptom scores or objective pulmonary functions during the drug and placebo periods. Four patients had significantly higher (p less than .05) and two patients significantly lower mean values for at least one of the pulmonary function tests during the isoproterenol period. The patient who is most likely to benefit from isoproterenol on a regular basis appears to have the following characteristics; (1) consistent improvement in pulmonary function tests 45 minutes after use of nebulized bronchodilator; (2) moderate rather than severe COLD; and (3) a relatively normal DLCO."}
{"id": "PMID:1092532", "title": "Postoperative bronchopleural fistula: clinical and experimental study.", "content": "It is a well-known fact that in pulmonary tuberculosis patients treated by resection, the quality of the suture material used for closing the bronchial stump plays an important role in the pathogenesis of postoperative bronchopleural fistula. Of 426 cases treated surgically and in whom silk suture thread was used, 23 developed bronchopleural fistula, whereas none of the 220 cases sutured with nylon monofilament developed abnormality. Statistical analysis of 100 surgical cases with silk thread suture and of 100 cases with nylon monofilament suture showed that the two groups had no marked differences as to background factors. Howevers, as compared with the silk-thread suture group, the nylon-monofilament suture group revealed more consistently favorable postoperative bronchoscopic findings. Experimental studies with dogs showed a similar lack of complications when the monofilament suture material was used, as contrasted were conducted in hospital by the same surgical personnel using the same procedures, it can be said that, to insure prevention of complications, the suture material for bronchial stump closure should be of non-irritating nature and preferably of monofilament strength and quality, such as nylon monofilament.", "contents": "Postoperative bronchopleural fistula: clinical and experimental study. It is a well-known fact that in pulmonary tuberculosis patients treated by resection, the quality of the suture material used for closing the bronchial stump plays an important role in the pathogenesis of postoperative bronchopleural fistula. Of 426 cases treated surgically and in whom silk suture thread was used, 23 developed bronchopleural fistula, whereas none of the 220 cases sutured with nylon monofilament developed abnormality. Statistical analysis of 100 surgical cases with silk thread suture and of 100 cases with nylon monofilament suture showed that the two groups had no marked differences as to background factors. Howevers, as compared with the silk-thread suture group, the nylon-monofilament suture group revealed more consistently favorable postoperative bronchoscopic findings. Experimental studies with dogs showed a similar lack of complications when the monofilament suture material was used, as contrasted were conducted in hospital by the same surgical personnel using the same procedures, it can be said that, to insure prevention of complications, the suture material for bronchial stump closure should be of non-irritating nature and preferably of monofilament strength and quality, such as nylon monofilament."}
{"id": "PMID:1092533", "title": "Treatment of cholerheic diarrhea with cholestyramine and a hydrophilic colloid.", "content": "Eight patients with ileal-resection diarrhea who were being treated with cholestyramine took part in a trial comparing the effectiveness of cholestyramine with that of a hydrophilic colloid. Both drugs reduced overall stool frequency and improved consistency, but a larger dose of colloid was required. One patient planned to use the hydrophilic colloid instead of cholestyramine.", "contents": "Treatment of cholerheic diarrhea with cholestyramine and a hydrophilic colloid. Eight patients with ileal-resection diarrhea who were being treated with cholestyramine took part in a trial comparing the effectiveness of cholestyramine with that of a hydrophilic colloid. Both drugs reduced overall stool frequency and improved consistency, but a larger dose of colloid was required. One patient planned to use the hydrophilic colloid instead of cholestyramine."}
{"id": "PMID:1092534", "title": "Low end-to-side rectoenteric anastomosis with single-layer wire.", "content": "A study of 41 cases where a single layer of 5-0 monofilament stainless steel wire is used for low end-to-side rectosigmoid or ileorectal anastomosis has shown satisfactory results. The procedure should be considered particularly where there is gross disparity between the sizes of the approximated cut ends.", "contents": "Low end-to-side rectoenteric anastomosis with single-layer wire. A study of 41 cases where a single layer of 5-0 monofilament stainless steel wire is used for low end-to-side rectosigmoid or ileorectal anastomosis has shown satisfactory results. The procedure should be considered particularly where there is gross disparity between the sizes of the approximated cut ends."}
{"id": "PMID:1092535", "title": "Simplified anterior resection: Use of the ta stapler.", "content": "Forty consecutive anterior resections using TA stapler were studied. No mortality, no clinical leak, and no infection were encountered in this group. The stapler is recommended as an aid in simplifying the operation and lessening the operative time.", "contents": "Simplified anterior resection: Use of the ta stapler. Forty consecutive anterior resections using TA stapler were studied. No mortality, no clinical leak, and no infection were encountered in this group. The stapler is recommended as an aid in simplifying the operation and lessening the operative time."}
{"id": "PMID:1092542", "title": "Alterations during the estrous cycle in the responsiveness of the pituitary to subcutaneous administration of synthetic LH-releasing hormone (LHRH).", "content": "To  further evaluate the alterations in responsiveness of the pituitary to synthetic LHRH during the proestrous discharge of gonadotropins, LHRH was given SC in the hope of producing a release of FSH as well as LH, and blood samples were removed prior to and at various intervals after the injections of the neurohormone while the rats were anesthetized with tribromoethanol. The procedure of anesthesia and blood sampling produced small declines in both FSH and LH, but these only achieved significance in the case of FSH on the morning of estrus and diestrus day 2. An increase in plasma LH occurred in response to LHRH at all stages of the estrous cycle but was minimal during diestrus. The increment in plasma LH was already increased by 9 AM on proestrus and the titer remained elevated for 2 h. A further increase in response to LHRH occured at 2 PM associated with an increase in initial plasma LH. The maximum response occurred at 5 PM together with maximal initial plasma LH, and the characteristics of the response changed such that there was a much larger increase in plasma LH at 20 min and a rapid decline thereafter, so that the response became pulselike. Initial plasma LH had already declined significantly by 9 PM, and this was associated with a dimished pulselike release of LH in response to LHRH. By the morning of estrus, an even smaller pulse-like release occurred. Significant FSH release in response to LHRH occurred at all stages of the cycle as indicated by the increments in plasma FSH. The relative increase in plasma titers was much less than that for LH and responsiveness to LHRH did not increase until 5 PM on proestrus. Responsiveness had already begun to decline by 9 PM on proestrus and by the morning of estrus was similar to that obtained on diestrus. Initial plasma FSH titers were first elevated by 2 PM on proestrus and they remained elevated on the morning of estrus. The results indicate that maximal responsiveness to LHRH in terms of LH release is associated with the proestrous discharge of LH, but that the maximal responsiveness in terms of FSH release terminates long before the endogenous release of hormone ceases sometime on estrus. The increased responsiveness in terms of LH release may be related to prior estrogen secretion and to the priming action of endogenous LHRH. The continued release of FSH in the face of a return of responsiveness to LHRH to low levels indicates that release of FSH on late proestrus and early estrus is not caused by endogenous release to LHRH.", "contents": "Alterations during the estrous cycle in the responsiveness of the pituitary to subcutaneous administration of synthetic LH-releasing hormone (LHRH). To  further evaluate the alterations in responsiveness of the pituitary to synthetic LHRH during the proestrous discharge of gonadotropins, LHRH was given SC in the hope of producing a release of FSH as well as LH, and blood samples were removed prior to and at various intervals after the injections of the neurohormone while the rats were anesthetized with tribromoethanol. The procedure of anesthesia and blood sampling produced small declines in both FSH and LH, but these only achieved significance in the case of FSH on the morning of estrus and diestrus day 2. An increase in plasma LH occurred in response to LHRH at all stages of the estrous cycle but was minimal during diestrus. The increment in plasma LH was already increased by 9 AM on proestrus and the titer remained elevated for 2 h. A further increase in response to LHRH occured at 2 PM associated with an increase in initial plasma LH. The maximum response occurred at 5 PM together with maximal initial plasma LH, and the characteristics of the response changed such that there was a much larger increase in plasma LH at 20 min and a rapid decline thereafter, so that the response became pulselike. Initial plasma LH had already declined significantly by 9 PM, and this was associated with a dimished pulselike release of LH in response to LHRH. By the morning of estrus, an even smaller pulse-like release occurred. Significant FSH release in response to LHRH occurred at all stages of the cycle as indicated by the increments in plasma FSH. The relative increase in plasma titers was much less than that for LH and responsiveness to LHRH did not increase until 5 PM on proestrus. Responsiveness had already begun to decline by 9 PM on proestrus and by the morning of estrus was similar to that obtained on diestrus. Initial plasma FSH titers were first elevated by 2 PM on proestrus and they remained elevated on the morning of estrus. The results indicate that maximal responsiveness to LHRH in terms of LH release is associated with the proestrous discharge of LH, but that the maximal responsiveness in terms of FSH release terminates long before the endogenous release of hormone ceases sometime on estrus. The increased responsiveness in terms of LH release may be related to prior estrogen secretion and to the priming action of endogenous LHRH. The continued release of FSH in the face of a return of responsiveness to LHRH to low levels indicates that release of FSH on late proestrus and early estrus is not caused by endogenous release to LHRH."}
{"id": "PMID:1092543", "title": "Quick release of FSH stimulated by repeated applications of synthetic luteinizing hormone-releasing hormone (LH-RH) in immature male rats.", "content": "Serum FSH and LH levels were estimated by radioimmunoassay following single subcutaneous administration, and repeated subcutaneous administration at various intervals in immature male rats. Single injection of LH-RH induced slight increase in FSH levels in comparison with considerable increase in LH. By two successive injections, the release of FSH was markedly stimulated when the time intervals were 15, 30, and 60 minutes,and rapidly elicited to release after the second injection. The greatest response was observed by two injections at 60 minutes interval. Moreover, the elevation of serum FSH level after the third injection was particularly remarkable, and the response was much stronger than that to the second injection. On the other hand, serum LH levels following the repeated injections were quite similar to that of FSH. And both FSH and LHwere linearly responded to doses of LH-RH repeatedly administered. These results indicated that the repeated quick administrations of LH-RH under appropriate intervals caused the acute high release of FSH, which was quite similar to LH release, and that these acute release of FSH and LH showed the dose-response relationship.", "contents": "Quick release of FSH stimulated by repeated applications of synthetic luteinizing hormone-releasing hormone (LH-RH) in immature male rats. Serum FSH and LH levels were estimated by radioimmunoassay following single subcutaneous administration, and repeated subcutaneous administration at various intervals in immature male rats. Single injection of LH-RH induced slight increase in FSH levels in comparison with considerable increase in LH. By two successive injections, the release of FSH was markedly stimulated when the time intervals were 15, 30, and 60 minutes,and rapidly elicited to release after the second injection. The greatest response was observed by two injections at 60 minutes interval. Moreover, the elevation of serum FSH level after the third injection was particularly remarkable, and the response was much stronger than that to the second injection. On the other hand, serum LH levels following the repeated injections were quite similar to that of FSH. And both FSH and LHwere linearly responded to doses of LH-RH repeatedly administered. These results indicated that the repeated quick administrations of LH-RH under appropriate intervals caused the acute high release of FSH, which was quite similar to LH release, and that these acute release of FSH and LH showed the dose-response relationship."}
{"id": "PMID:1092544", "title": "Immobilized L-asparginase embedded in fibrin polymer.", "content": "Immobilized asparaginase was prepared by embedding asparaginase (which is effective for remission in children with leukemia) into fibrin polymer formed by fibrinogen-fibrin conversion in the presence of thrombin. The immobilized asparaginase film did not dissolve in 6 mol/1 urea, suggesting that blood coagulation factor XIII participates in the cross-linking between fibrins and between fibrin and asparaginase.", "contents": "Immobilized L-asparginase embedded in fibrin polymer. Immobilized asparaginase was prepared by embedding asparaginase (which is effective for remission in children with leukemia) into fibrin polymer formed by fibrinogen-fibrin conversion in the presence of thrombin. The immobilized asparaginase film did not dissolve in 6 mol/1 urea, suggesting that blood coagulation factor XIII participates in the cross-linking between fibrins and between fibrin and asparaginase."}
{"id": "PMID:1092540", "title": "Platelets, thrombosis and drugs.", "content": "The development of thrombosis involves 4 main factors: the vessel wall, the formed elements of the blood, blood coagulation, and blood flow. In venous thrombosis, however, the major part in both the initiation and growth of thrombi is played by the platelets. In selecting drugs which inhibit platelet function it is helful to know which of the platelet reactions that contribute to thrombus formation can be inhibited by various agents. Platelets adhere to the damaged vessel wall, collagen being probably the most important constituent involved. They are then stimulated to release the contents of their storage granules. Release-inducing agents promote the discharge of adenosine diphosphate (ADP) which causes platelets in the vicinity to swell to a more spherical shape, extend pseudopods and adhere to each other. Platelet aggregation is reversible, and a number of drugs have been shown to be capable of inhibiting platelet function at various stages, both in vitro and in vivo. Adrenaline, noradrenaline, oestrogens and nicotine enhance aggregation. Drugs which inhibit platelet function include the non-steroidal anti-inflammatory drugs, the pyrimido-pyrimidines (e.g. dipyridamole), hydroxychloroquine, clofibrate, and dextran. In this review the effects of drugs which inhibit platelet function are outlined and the extent to which they can be used to influence the course of thromboembolic disease in man is discussed. It is suggested that combination of anti-platelet drugs with anticoagulants could prove clinically useful.", "contents": "Platelets, thrombosis and drugs. The development of thrombosis involves 4 main factors: the vessel wall, the formed elements of the blood, blood coagulation, and blood flow. In venous thrombosis, however, the major part in both the initiation and growth of thrombi is played by the platelets. In selecting drugs which inhibit platelet function it is helful to know which of the platelet reactions that contribute to thrombus formation can be inhibited by various agents. Platelets adhere to the damaged vessel wall, collagen being probably the most important constituent involved. They are then stimulated to release the contents of their storage granules. Release-inducing agents promote the discharge of adenosine diphosphate (ADP) which causes platelets in the vicinity to swell to a more spherical shape, extend pseudopods and adhere to each other. Platelet aggregation is reversible, and a number of drugs have been shown to be capable of inhibiting platelet function at various stages, both in vitro and in vivo. Adrenaline, noradrenaline, oestrogens and nicotine enhance aggregation. Drugs which inhibit platelet function include the non-steroidal anti-inflammatory drugs, the pyrimido-pyrimidines (e.g. dipyridamole), hydroxychloroquine, clofibrate, and dextran. In this review the effects of drugs which inhibit platelet function are outlined and the extent to which they can be used to influence the course of thromboembolic disease in man is discussed. It is suggested that combination of anti-platelet drugs with anticoagulants could prove clinically useful."}
{"id": "PMID:1092545", "title": "The involvement of sulphydryl groups in the peptidyl transferase centre of eukaryotic ribosomes.", "content": "Treatment of mammalian ribosomes with N-ethylmaleimide enhances up to 100% the ribosome efficiency in the \"fragment reaction assay\" for peptide bond formation by increasing the affinity of the substrate C-A-C-C-A-Leu-Ac for the donor site. This stimulation in peptidyl transferase activity was not observed when yeast ribosomes were treated in a similar manner. Stimulation of the peptidyl transferase activity of mammalian ribosomes was also observed by treatment with either p-chloromercuribenzoic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) or 5,5'-dithiobis-(2-nitropyridine) or the maleimide-derived antibiotic showdomycin. N-Ethylmaleimide treatment also enhances C-A-C-C-A-Leu binding to the acceptor site of the peptidyl transferase centre. However, neither binding of N-Ac-Phe-tRNA in the presence of ethanol, nor binding of Phe-tRNA to the ribosomes is stimulated by N-ethylmaleimide. The antibiotic tenuazionic acid (a selective inhibitor of peptide bond formation by mammalian ribosomes) appears to require for its inhibitory effect the ribosome sulphydryl residues, since its inhibitory action on the fragment reaction is greatly decreased in ribosomes treated with N-ethylmaleimide.", "contents": "The involvement of sulphydryl groups in the peptidyl transferase centre of eukaryotic ribosomes. Treatment of mammalian ribosomes with N-ethylmaleimide enhances up to 100% the ribosome efficiency in the \"fragment reaction assay\" for peptide bond formation by increasing the affinity of the substrate C-A-C-C-A-Leu-Ac for the donor site. This stimulation in peptidyl transferase activity was not observed when yeast ribosomes were treated in a similar manner. Stimulation of the peptidyl transferase activity of mammalian ribosomes was also observed by treatment with either p-chloromercuribenzoic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) or 5,5'-dithiobis-(2-nitropyridine) or the maleimide-derived antibiotic showdomycin. N-Ethylmaleimide treatment also enhances C-A-C-C-A-Leu binding to the acceptor site of the peptidyl transferase centre. However, neither binding of N-Ac-Phe-tRNA in the presence of ethanol, nor binding of Phe-tRNA to the ribosomes is stimulated by N-ethylmaleimide. The antibiotic tenuazionic acid (a selective inhibitor of peptide bond formation by mammalian ribosomes) appears to require for its inhibitory effect the ribosome sulphydryl residues, since its inhibitory action on the fragment reaction is greatly decreased in ribosomes treated with N-ethylmaleimide."}
{"id": "PMID:1092541", "title": "Diuretics: mechanism of action and clinical application.", "content": "Despite the bewildering number of diuretics available to the physician, these drugs can be divided into 4 main groups, characterised by their site of action on sodium reabsorption in the kidney. Drugs acting on the ascending limb of the loop of Henle have a powerful but short acting diuretic effect; they include frusemide, ethacrynic acid and bumetanide. The benzothiadiazines and related compounds have a moderate diuretic action spread over a longer period, whilst the potassium-sparing diuretics, triamterene, amiloride and spironolactone, have only a weak diuretic effect but a marked ability to diminish urinary potassium excretion. The fourth group is made up of miscellaneous substances which function as vasodilator or osmotic agents. The pathogenesis of oedema formation in heart failure is outlined and a logical approach to treatment suggested. Duiretics are being increasingly used in the treatment of non-oedematous states, in particular hypertension, diabetes insipidus and hypercalciuria; their exact role in pregnancy and acute renal failure remains controversial. Side-effects can be related to their effect on electrolyte excretion and include hypokalaemia, hyponatraemia, hyperkalaemia and hyperuricaemia. The incidence of disturbed carbohydrate tolerance in previously normal individuals is low. Other less common side-effects are also discussed.", "contents": "Diuretics: mechanism of action and clinical application. Despite the bewildering number of diuretics available to the physician, these drugs can be divided into 4 main groups, characterised by their site of action on sodium reabsorption in the kidney. Drugs acting on the ascending limb of the loop of Henle have a powerful but short acting diuretic effect; they include frusemide, ethacrynic acid and bumetanide. The benzothiadiazines and related compounds have a moderate diuretic action spread over a longer period, whilst the potassium-sparing diuretics, triamterene, amiloride and spironolactone, have only a weak diuretic effect but a marked ability to diminish urinary potassium excretion. The fourth group is made up of miscellaneous substances which function as vasodilator or osmotic agents. The pathogenesis of oedema formation in heart failure is outlined and a logical approach to treatment suggested. Duiretics are being increasingly used in the treatment of non-oedematous states, in particular hypertension, diabetes insipidus and hypercalciuria; their exact role in pregnancy and acute renal failure remains controversial. Side-effects can be related to their effect on electrolyte excretion and include hypokalaemia, hyponatraemia, hyperkalaemia and hyperuricaemia. The incidence of disturbed carbohydrate tolerance in previously normal individuals is low. Other less common side-effects are also discussed."}
{"id": "PMID:1092546", "title": "Properties of ATP and UTP analogues with P-S-C-5' bonds.", "content": "Analogues of ATP and UTP bearing C-5'-S-P ester bonds were found not to be substrates but weak competitive inhibitors of Escherichia coli RNA polymerase. The K-i values of the analogues obtained in the transcription of poly[d(A-T)] or poly(dT) under various conditions are in the order of millimolar. Evidence was derived from proton magnetic resonance spectra that nucleotides with C-5'-S-P bonds do not exist in gauche-gauche conformation normally adopted by natural occurring nucleotides. This leads us to assume that the gauche-gauche conformation is an essental prerequisite for substrates of RNA polymerase. Ado-5'-S-PPP substituted for ATP as substrate of hexokinase from yeast rather effectively thus indicating that a distinct stereochemical orientation of the alpha-phosphate ester bond is not a stringent requirement for substrates of this enzyme.", "contents": "Properties of ATP and UTP analogues with P-S-C-5' bonds. Analogues of ATP and UTP bearing C-5'-S-P ester bonds were found not to be substrates but weak competitive inhibitors of Escherichia coli RNA polymerase. The K-i values of the analogues obtained in the transcription of poly[d(A-T)] or poly(dT) under various conditions are in the order of millimolar. Evidence was derived from proton magnetic resonance spectra that nucleotides with C-5'-S-P bonds do not exist in gauche-gauche conformation normally adopted by natural occurring nucleotides. This leads us to assume that the gauche-gauche conformation is an essental prerequisite for substrates of RNA polymerase. Ado-5'-S-PPP substituted for ATP as substrate of hexokinase from yeast rather effectively thus indicating that a distinct stereochemical orientation of the alpha-phosphate ester bond is not a stringent requirement for substrates of this enzyme."}
{"id": "PMID:1092547", "title": "Determination of the kinetic constants of glucose-6-phosphate 1-epimerase by non-linear optimization.", "content": "1. The overall kinetic constants of the reversible anomerisation of d-glucopyranose 6-phosphate from alpha to beta non-enzymatically as well as catalysed by glucose-6-phosphate 1-epimerase are determined by application of a novel computerized non-linear optimization technique. 2. The non-enzymic rate constants for the anomerisation of d-glucopyranose 6-phosphate from alpha to beta and reverse are 0.0658 and 0.0389s-minus 1, respectively. The Michaelis constants of the enzymic reaction are (see journal for formulas) with the turnover numbers of 1950s-minus 1 and 446s-minus 1 for the conversion of d-glucopyranose 6-phosphate from alpha to beta and reverse, respectively.", "contents": "Determination of the kinetic constants of glucose-6-phosphate 1-epimerase by non-linear optimization. 1. The overall kinetic constants of the reversible anomerisation of d-glucopyranose 6-phosphate from alpha to beta non-enzymatically as well as catalysed by glucose-6-phosphate 1-epimerase are determined by application of a novel computerized non-linear optimization technique. 2. The non-enzymic rate constants for the anomerisation of d-glucopyranose 6-phosphate from alpha to beta and reverse are 0.0658 and 0.0389s-minus 1, respectively. The Michaelis constants of the enzymic reaction are (see journal for formulas) with the turnover numbers of 1950s-minus 1 and 446s-minus 1 for the conversion of d-glucopyranose 6-phosphate from alpha to beta and reverse, respectively."}
{"id": "PMID:1092548", "title": "Left ventricular systolic time intervals during acute myocardial infarction.", "content": "Left ventricular systolic time intervals (LVSTI) were measured several times daily during 10 days in 47 patients with acute myocardial infarction without major complications. Left ventricular ejection time (LVET) and the interval between the beginning of depolarization and the aortic component of the second heart sound (Q-A2) decreased progressively during the first 72 hr. Shortening of Q--A2 and LVET was most marked in patients with heart failure and persisted till the end of the observation period. Q-upstroke (Q-U) prolonged progressively during the first 3 days, mainly in patients with heart failure. After 10 days, Q-U tended to return to normal except in decompensated patients. Definite diurnal variation in LVSTI were observed; LVET and Q--A2 were longest in the morning hours. Multiple regression analysis of LVSTI with a series of clinical variables revealed that R-R interval, age, sex, digitalis administration, day after infarction, hour of the day, peak of SGOT, and survival are significantly and independently correlated with the changes in LVSTI.", "contents": "Left ventricular systolic time intervals during acute myocardial infarction. Left ventricular systolic time intervals (LVSTI) were measured several times daily during 10 days in 47 patients with acute myocardial infarction without major complications. Left ventricular ejection time (LVET) and the interval between the beginning of depolarization and the aortic component of the second heart sound (Q-A2) decreased progressively during the first 72 hr. Shortening of Q--A2 and LVET was most marked in patients with heart failure and persisted till the end of the observation period. Q-upstroke (Q-U) prolonged progressively during the first 3 days, mainly in patients with heart failure. After 10 days, Q-U tended to return to normal except in decompensated patients. Definite diurnal variation in LVSTI were observed; LVET and Q--A2 were longest in the morning hours. Multiple regression analysis of LVSTI with a series of clinical variables revealed that R-R interval, age, sex, digitalis administration, day after infarction, hour of the day, peak of SGOT, and survival are significantly and independently correlated with the changes in LVSTI."}
{"id": "PMID:1092549", "title": "Effects of the intra-arterial injection of bradykinin into the limbs, upon the activity of mesencephalic reticular units.", "content": "The changes in firing rate of mesencephalic reticular units after intra-arterial injection into the limbs of a potent nociceptive agent, bradykinin, were studied in cats (unanesthetized, immobilized with flaxedil and hyperventilated). 30 per cent of the d35 studied cells were affected, 56 per cent were excited, 23 per cent inhibited and 5 per cent had mixed effects. Among the 75 excited cells, the activation of 16 of them seemed to related to the arousa- processes (group A); for 56 cells the increase seemed dire-tly dependent on the nociceptive stimulation itself (group B). The changes of firing rate were repruducible; their latencies and durations were of the same order as the latencies and duration of the nociceptive reactions and painful sensation s, which have been obtained in animals and men after bradykinin injections. The modifications induced by bradykinin administration were suppressed by Ketamin and Thiopental.", "contents": "Effects of the intra-arterial injection of bradykinin into the limbs, upon the activity of mesencephalic reticular units. The changes in firing rate of mesencephalic reticular units after intra-arterial injection into the limbs of a potent nociceptive agent, bradykinin, were studied in cats (unanesthetized, immobilized with flaxedil and hyperventilated). 30 per cent of the d35 studied cells were affected, 56 per cent were excited, 23 per cent inhibited and 5 per cent had mixed effects. Among the 75 excited cells, the activation of 16 of them seemed to related to the arousa- processes (group A); for 56 cells the increase seemed dire-tly dependent on the nociceptive stimulation itself (group B). The changes of firing rate were repruducible; their latencies and durations were of the same order as the latencies and duration of the nociceptive reactions and painful sensation s, which have been obtained in animals and men after bradykinin injections. The modifications induced by bradykinin administration were suppressed by Ketamin and Thiopental."}
{"id": "PMID:1092560", "title": "Ethical and social aspects of experimental gene manipulation.", "content": "New genetic techniques for the isolation and rejoining of segments of DNA now permit construction of biologically active recombinant DNA molecules. Such recombinant DNA molecules can be multiplied many times by inserting them into the bacterium Escherichia coli. Thus, we now possess a powerful technology for cloning segments of DNA from any source and for adding new genetic capabilities to bacteria. Application of this technology promises to facilitate the solution of a variety of theoretical and practical biological questions. However, use of this technology will also result in the creation of novel organisms whose biological properties may not be completely predictable in advance. Some of these organisms may be hazardous to man or to the environment. This paper discusses some ethical and social control of the new genetic technologies.", "contents": "Ethical and social aspects of experimental gene manipulation. New genetic techniques for the isolation and rejoining of segments of DNA now permit construction of biologically active recombinant DNA molecules. Such recombinant DNA molecules can be multiplied many times by inserting them into the bacterium Escherichia coli. Thus, we now possess a powerful technology for cloning segments of DNA from any source and for adding new genetic capabilities to bacteria. Application of this technology promises to facilitate the solution of a variety of theoretical and practical biological questions. However, use of this technology will also result in the creation of novel organisms whose biological properties may not be completely predictable in advance. Some of these organisms may be hazardous to man or to the environment. This paper discusses some ethical and social control of the new genetic technologies."}
{"id": "PMID:1092561", "title": "Incidence of pregnancy, changes in menstrual pattern, and recovery of endometrial function after discontinuation of medroxyprogesterone acetate therapy.", "content": "The incidence of pregnancy, recovery of endometrial function, and changes in menstrual pattern were studied in 62 women who had received two or more intramuscular injections (150 mg) of medroxyprogesterone acetate (Depo-Provera) every three months and oral diethylstilbestrol (1 mg) daily for seven days every month. Immediately after discontinuing this therapy, 71% of the patients had regular menstrual cycles, 10% had irregular cycles, and 19% had amenorrhea. All the patients had regular cycles by the end of one year except one women who was probably menopausal. The average time required to conceive after stopping therapy was 9.3 months, range, 3 to 21 months). Conception was definitely delayed as shown by endometrial biopsies and the incidence of pregnancy. Within six months, 25% of the patients conceived and within one year, 69% conceived. By two years, four had not conceived. The percentage of pregnancies seemed to decrease with increased patient age.", "contents": "Incidence of pregnancy, changes in menstrual pattern, and recovery of endometrial function after discontinuation of medroxyprogesterone acetate therapy. The incidence of pregnancy, recovery of endometrial function, and changes in menstrual pattern were studied in 62 women who had received two or more intramuscular injections (150 mg) of medroxyprogesterone acetate (Depo-Provera) every three months and oral diethylstilbestrol (1 mg) daily for seven days every month. Immediately after discontinuing this therapy, 71% of the patients had regular menstrual cycles, 10% had irregular cycles, and 19% had amenorrhea. All the patients had regular cycles by the end of one year except one women who was probably menopausal. The average time required to conceive after stopping therapy was 9.3 months, range, 3 to 21 months). Conception was definitely delayed as shown by endometrial biopsies and the incidence of pregnancy. Within six months, 25% of the patients conceived and within one year, 69% conceived. By two years, four had not conceived. The percentage of pregnancies seemed to decrease with increased patient age."}
{"id": "PMID:1092562", "title": "Preliminary human trial of a new male sterilization procedure: vas sclerosing.", "content": "A new procedure developed in animals has been applied to produce male human sterilization. This involves the percutaneous injection of an ethanol and formaldehyde solution into the vasa deferentia. An initial clinical trial of eight human volunteers indicates that this is an effective method of producing male sterilization.", "contents": "Preliminary human trial of a new male sterilization procedure: vas sclerosing. A new procedure developed in animals has been applied to produce male human sterilization. This involves the percutaneous injection of an ethanol and formaldehyde solution into the vasa deferentia. An initial clinical trial of eight human volunteers indicates that this is an effective method of producing male sterilization."}
{"id": "PMID:1092574", "title": "Influence of repeated local stimulation on the splenic response to mucosal and parenteral immunization.", "content": "It has been suggested that a regional immune response may be induced by local administration of small doses of antigen, whereas larger doses are capable of inducing not only a local but also a systemic immune response. Except with fluorescence, the study at the cellular level and in the mucosa of specific antibody-producing cells appears difficult. The circulation in the body of lymphocytes from the lamina propria of the gut allows the detection of antibody-producing cells in extra-intestinal lymphoid tissues. The administration of 4 times 10-9 sheep red blood cells (SRBC) by gastric intubation to BALB/c mice (repeated daily for 4 days) was found to cause the appearance in the spleen of plaque-forming cells which predominantly belonged to the IgA class and whose numbers reached a maximum at about day 8 or 9 after the onset of the immunization. This observation is relevant to splenic colonies from cells that had received their antigenic stimulation in the gut wall and thence had seeded out in the body. Booster administration of SRBC performed 3 months after the first antigenic contact induced in the spleen an immunological response similar to that observed in newly primed mice and in no way suggestive of immunologic memory. A secondary response is completely absent when the second intragastric immunization is initiated 10 days after the end of the first. The parenteral administration of 2 times 10-8 SRBC to mice primed by the oral route 15 days earlier is followed by a marked hyporeactivity of the spleen. In any case, the IgA system appears endowed with only a minimum, if any, immunological memory to non-living antigen. In some conditions local immunological stimulation may even cause tolerance.", "contents": "Influence of repeated local stimulation on the splenic response to mucosal and parenteral immunization. It has been suggested that a regional immune response may be induced by local administration of small doses of antigen, whereas larger doses are capable of inducing not only a local but also a systemic immune response. Except with fluorescence, the study at the cellular level and in the mucosa of specific antibody-producing cells appears difficult. The circulation in the body of lymphocytes from the lamina propria of the gut allows the detection of antibody-producing cells in extra-intestinal lymphoid tissues. The administration of 4 times 10-9 sheep red blood cells (SRBC) by gastric intubation to BALB/c mice (repeated daily for 4 days) was found to cause the appearance in the spleen of plaque-forming cells which predominantly belonged to the IgA class and whose numbers reached a maximum at about day 8 or 9 after the onset of the immunization. This observation is relevant to splenic colonies from cells that had received their antigenic stimulation in the gut wall and thence had seeded out in the body. Booster administration of SRBC performed 3 months after the first antigenic contact induced in the spleen an immunological response similar to that observed in newly primed mice and in no way suggestive of immunologic memory. A secondary response is completely absent when the second intragastric immunization is initiated 10 days after the end of the first. The parenteral administration of 2 times 10-8 SRBC to mice primed by the oral route 15 days earlier is followed by a marked hyporeactivity of the spleen. In any case, the IgA system appears endowed with only a minimum, if any, immunological memory to non-living antigen. In some conditions local immunological stimulation may even cause tolerance."}
{"id": "PMID:1092575", "title": "Assessment of the efficacy of influenza vaccines against natural challenge.", "content": "Assessment of influenza vaccines by serological conversion rates or by protection against artificial challenge in animals or man give only a preliminary and indirect guide to their expected efficiency in controlling the natural epidemic disease. Field trials are essential but demand careful advance planning and control before meaningful results can be obtained. This paper reviews critically the various attempts that have been made to establish an ideal trial procedure to overcome the many inherent difficulties in studying 'wild' influenza. Vaccines of appropriate antigenic content can prevent influenza in 75 percent or more of recipients for up to a year, and can decrease the severity and duration of the disease in those who do succumb. Attack rates can be reduced even in epidemics occurring within 8-14 days of vaccination. If a high proportion of a population is vaccinated, infection rates in the unvaccinated in the same environment may fall. The desirability of vaccinating (a) only high risk groups or (b) entire open population or (c) school children is discussed.", "contents": "Assessment of the efficacy of influenza vaccines against natural challenge. Assessment of influenza vaccines by serological conversion rates or by protection against artificial challenge in animals or man give only a preliminary and indirect guide to their expected efficiency in controlling the natural epidemic disease. Field trials are essential but demand careful advance planning and control before meaningful results can be obtained. This paper reviews critically the various attempts that have been made to establish an ideal trial procedure to overcome the many inherent difficulties in studying 'wild' influenza. Vaccines of appropriate antigenic content can prevent influenza in 75 percent or more of recipients for up to a year, and can decrease the severity and duration of the disease in those who do succumb. Attack rates can be reduced even in epidemics occurring within 8-14 days of vaccination. If a high proportion of a population is vaccinated, infection rates in the unvaccinated in the same environment may fall. The desirability of vaccinating (a) only high risk groups or (b) entire open population or (c) school children is discussed."}
{"id": "PMID:1092576", "title": "An attenuated influenza virus vaccine: reactogenicity, transmissibility, immunogenicity and protective efficacy.", "content": "A more effective influenza virus vaccine must be capable of inducing a greater degree of protection against the disease while causing fewer unwanted side effects. If the vaccine is an attenuated one, it should not be transmissible unless it can incontrovertably be shown to be genetically stable and not mutate to a more virulent form. One approach for producing an attenuated strain has been to select for horse serum inhibitor resistance. Such a strain (ANN strain, Recherche et Industrie Th\u00e9rapeutique) was tested for the above characteristics. The vaccine (containing 10-7 50 percent egg infectious doses) and placebo was administered in a double-blind fashion to 37 volunteers in 2 doses 2-3 weeks apart. Volunteers were housed so that a vaccinee and control shared a room for 4 days following immunization. The only side effects noted were mild rhinorrhea and sore throat in 3 vaccinees. There was no evidence of vaccine virus having been transmitted from a vaccinee to a control individual.A 4-fold or greater rise in serum antibody was noted in 13/19 vaccinees following either the 1st or 2nd dose. Only 1/18 controls showed such a rise. A similar nasal antibody rise was noted in 15/19 and 1/18 of the vaccine and placebo groups respectively. Following virulent challenge 7 of 18 in the control group, and 1 of 19 vaccinees, became ill.", "contents": "An attenuated influenza virus vaccine: reactogenicity, transmissibility, immunogenicity and protective efficacy. A more effective influenza virus vaccine must be capable of inducing a greater degree of protection against the disease while causing fewer unwanted side effects. If the vaccine is an attenuated one, it should not be transmissible unless it can incontrovertably be shown to be genetically stable and not mutate to a more virulent form. One approach for producing an attenuated strain has been to select for horse serum inhibitor resistance. Such a strain (ANN strain, Recherche et Industrie Th\u00e9rapeutique) was tested for the above characteristics. The vaccine (containing 10-7 50 percent egg infectious doses) and placebo was administered in a double-blind fashion to 37 volunteers in 2 doses 2-3 weeks apart. Volunteers were housed so that a vaccinee and control shared a room for 4 days following immunization. The only side effects noted were mild rhinorrhea and sore throat in 3 vaccinees. There was no evidence of vaccine virus having been transmitted from a vaccinee to a control individual.A 4-fold or greater rise in serum antibody was noted in 13/19 vaccinees following either the 1st or 2nd dose. Only 1/18 controls showed such a rise. A similar nasal antibody rise was noted in 15/19 and 1/18 of the vaccine and placebo groups respectively. Following virulent challenge 7 of 18 in the control group, and 1 of 19 vaccinees, became ill."}
{"id": "PMID:1092577", "title": "The coronaviruses.", "content": "Coronaviruses of human origin have emerged as probable important etiologic agents of acute upper respiratory tract illnesses in adults; their role in the etiology of respiratory illnesses of childhood is less certain. The first coronavirus of human origin, B814, was described in 1965 and since that time only 31 additional strains have been recovered. Ten of these were originally recovered in human embryonic tracheal organ culturesonly, and the remainder in monolayer cell cultures. All of the latter group were antigenically related to a prototype strain designated 229e. As a result of the fastidious growth requirements of these agents, most of the knowledge concerning the clinical syndromes associated with them has come from chajlenge studies in volunteers and seroepidemiologic investigations. Coronaviruses have been shown to be associated with a substantial number of adult respiratory illnesses especially during certain periods when rhinovirus infections are infrequent. Progress in propagating coronaviruses and in detecting coronavirus infections has been achieved recently: viruses similar to 229E were successfully recovered in human embryonic intestine cell cultures from patients with upper respiratory tract illnesses; 30C strains (B814, LP and EVS) were recovered in L132 cell cultures from infectious nasal washings; OC43(and OC38) viruses which were originally recovered in OC and later adapted to grow in suckling mice were not only shown to directly hemagglutinate various erythrocytes but were also adapted to grow in MK cell cultures; OC43 virus was also found to induce hemadsorption of rat and mouse erythrocytes in certain cell cultures; and a coronavirus strain was detected by immune electron microscopy. Coronaviruses are also associated with certain diseases in animals.", "contents": "The coronaviruses. Coronaviruses of human origin have emerged as probable important etiologic agents of acute upper respiratory tract illnesses in adults; their role in the etiology of respiratory illnesses of childhood is less certain. The first coronavirus of human origin, B814, was described in 1965 and since that time only 31 additional strains have been recovered. Ten of these were originally recovered in human embryonic tracheal organ culturesonly, and the remainder in monolayer cell cultures. All of the latter group were antigenically related to a prototype strain designated 229e. As a result of the fastidious growth requirements of these agents, most of the knowledge concerning the clinical syndromes associated with them has come from chajlenge studies in volunteers and seroepidemiologic investigations. Coronaviruses have been shown to be associated with a substantial number of adult respiratory illnesses especially during certain periods when rhinovirus infections are infrequent. Progress in propagating coronaviruses and in detecting coronavirus infections has been achieved recently: viruses similar to 229E were successfully recovered in human embryonic intestine cell cultures from patients with upper respiratory tract illnesses; 30C strains (B814, LP and EVS) were recovered in L132 cell cultures from infectious nasal washings; OC43(and OC38) viruses which were originally recovered in OC and later adapted to grow in suckling mice were not only shown to directly hemagglutinate various erythrocytes but were also adapted to grow in MK cell cultures; OC43 virus was also found to induce hemadsorption of rat and mouse erythrocytes in certain cell cultures; and a coronavirus strain was detected by immune electron microscopy. Coronaviruses are also associated with certain diseases in animals."}
{"id": "PMID:1092578", "title": "Contagious bovine pleuropneumonia-protection following natural infection and vaccination.", "content": "Cattle that recover from contagious bovine pleuropneumonia, the disease caused by Mycoplasma mycoides var. mycoides, are usually immune to reinfection. Prohylactic inoculation against the disease has been practiced for over a century and utilizes the fact that even virulent strains of M. mycoides inoculated subcutaneously into fully susceptible cattle only very rarely produce pneumonia, but instead produce subcutaneous lesions which may or may not kill the animals depending on various factors including the site of the inoculation and virulence of the strains. Animals that survive are immune to further local infections or to the natural disease. The most successful vaccines at present consist of live attenuated strains of M. mycoides which produce a slight local reaction at the site of inoculation. It appears that vaccines made from inactivated organisms are poorly immunogenic and may even sensitize the animals thereby causing them to react more severely to subsequent challenge.", "contents": "Contagious bovine pleuropneumonia-protection following natural infection and vaccination. Cattle that recover from contagious bovine pleuropneumonia, the disease caused by Mycoplasma mycoides var. mycoides, are usually immune to reinfection. Prohylactic inoculation against the disease has been practiced for over a century and utilizes the fact that even virulent strains of M. mycoides inoculated subcutaneously into fully susceptible cattle only very rarely produce pneumonia, but instead produce subcutaneous lesions which may or may not kill the animals depending on various factors including the site of the inoculation and virulence of the strains. Animals that survive are immune to further local infections or to the natural disease. The most successful vaccines at present consist of live attenuated strains of M. mycoides which produce a slight local reaction at the site of inoculation. It appears that vaccines made from inactivated organisms are poorly immunogenic and may even sensitize the animals thereby causing them to react more severely to subsequent challenge."}
{"id": "PMID:1092579", "title": "The importance of mycoplasmas in respiratory infections.", "content": "In contrast to the uncertain role of mycoplasmas in genital disease, there is no doubt that they produce respiratory disease. The first mycoplasma isolated, M. mycoidesis is the aetiological agent of bovine pleuropneumonia, a disease now confined to parts of Africa and Australia. M. capri causes a similar disease in goats which is geographically more widespread. Enzootic pneumonia of pigs, seen in various parts of the world, is due primarily to M. suipneumoniae. Although some strains of M. hyorhinis also seem capable of causing disease. Not all strains of a mycoplasma species are equally pathogenic; only some strains of M. pulmonis produce severe respiratory disease in mice and rats. Further, concomitant infection with other agents may result in more severe disease; this is particularly seen in M. gallisepticum infections of poultry. Of those mycoplasmas which infect man, only M. pneumoniae is known to cause respiratory disease, mainly involving the younger age groups. Mycoplasmas spread by close contact so that disease is seen in family groups or where there is crowding or herding. Factors in resistance to respiratory disease caused by mycoplasmas have been poorly understood but the importance of local immunity is becoming more clear.", "contents": "The importance of mycoplasmas in respiratory infections. In contrast to the uncertain role of mycoplasmas in genital disease, there is no doubt that they produce respiratory disease. The first mycoplasma isolated, M. mycoidesis is the aetiological agent of bovine pleuropneumonia, a disease now confined to parts of Africa and Australia. M. capri causes a similar disease in goats which is geographically more widespread. Enzootic pneumonia of pigs, seen in various parts of the world, is due primarily to M. suipneumoniae. Although some strains of M. hyorhinis also seem capable of causing disease. Not all strains of a mycoplasma species are equally pathogenic; only some strains of M. pulmonis produce severe respiratory disease in mice and rats. Further, concomitant infection with other agents may result in more severe disease; this is particularly seen in M. gallisepticum infections of poultry. Of those mycoplasmas which infect man, only M. pneumoniae is known to cause respiratory disease, mainly involving the younger age groups. Mycoplasmas spread by close contact so that disease is seen in family groups or where there is crowding or herding. Factors in resistance to respiratory disease caused by mycoplasmas have been poorly understood but the importance of local immunity is becoming more clear."}
{"id": "PMID:1092580", "title": "Multiple metabolic functions of glucose in rat pancreatic islets.", "content": "Metabolic interactions between glucose and amino acids were studied with isolated rat islets using glucose utilization and lactate formation as indicators. Certain amino acids (8-10 mM) are capable of greatly stimulating lactate formation from 5mM glucose. On a molar basis L-isoleucine is the most potent stimulator in a group of twenty-six amino acids. Aphysiological amino acid mixture (7.5-14 mM) or L-isoleucine (8 mM) profoundly altered the basic sigmoidal relation between glucose concentration in the medium and the rate of glucose utilization and lactate formation: with basal glucose (5 mM) both glucose utilization and lactate production were stimulated by the amino ACID MIXTURE and by L-isoleucine; at high glucose levels utilization was decreased by the amino acid mixture, but was unaffected by L-isoleucine, whereas lactate formation was decreased by both additions. The data indicate that amino acids may play a significant role in regulating the extent to which glucose serves as a fuel of pancreatic islet cells and in determining the pathways of glucose metabolism. In order to elucidate the mechanisms of the amino acid effect, studies with phloridzin, ouabain, iodoacetate, cytochalasin B, and Na+-deficiency were performed with the most effective amino acid, L-isoleucine. Each of these agents and Na+-deficiency substantially reduced or completely blocked the extra lactate formation induced by L-isoleucine (8-10 mM). The intracellular uptake of 14-CL-isoleucine by isolated islets was found to be Na+-independent, and uphill transport of this amino acid was not detectable, whether basal glucose was present in the medium or not. The action of iodoacetate in blocking glycolysis was reinvestigated. After forty-five minutes of exposure, 0.2mM iodoacetate completely blocks lactate formation as well as glucose utilization. Thisconfirms and extends earlier data for this laboratory and suggests that this SH-reagent indeed allows dissociation of the fuel and releasing functions of glucose.", "contents": "Multiple metabolic functions of glucose in rat pancreatic islets. Metabolic interactions between glucose and amino acids were studied with isolated rat islets using glucose utilization and lactate formation as indicators. Certain amino acids (8-10 mM) are capable of greatly stimulating lactate formation from 5mM glucose. On a molar basis L-isoleucine is the most potent stimulator in a group of twenty-six amino acids. Aphysiological amino acid mixture (7.5-14 mM) or L-isoleucine (8 mM) profoundly altered the basic sigmoidal relation between glucose concentration in the medium and the rate of glucose utilization and lactate formation: with basal glucose (5 mM) both glucose utilization and lactate production were stimulated by the amino ACID MIXTURE and by L-isoleucine; at high glucose levels utilization was decreased by the amino acid mixture, but was unaffected by L-isoleucine, whereas lactate formation was decreased by both additions. The data indicate that amino acids may play a significant role in regulating the extent to which glucose serves as a fuel of pancreatic islet cells and in determining the pathways of glucose metabolism. In order to elucidate the mechanisms of the amino acid effect, studies with phloridzin, ouabain, iodoacetate, cytochalasin B, and Na+-deficiency were performed with the most effective amino acid, L-isoleucine. Each of these agents and Na+-deficiency substantially reduced or completely blocked the extra lactate formation induced by L-isoleucine (8-10 mM). The intracellular uptake of 14-CL-isoleucine by isolated islets was found to be Na+-independent, and uphill transport of this amino acid was not detectable, whether basal glucose was present in the medium or not. The action of iodoacetate in blocking glycolysis was reinvestigated. After forty-five minutes of exposure, 0.2mM iodoacetate completely blocks lactate formation as well as glucose utilization. Thisconfirms and extends earlier data for this laboratory and suggests that this SH-reagent indeed allows dissociation of the fuel and releasing functions of glucose."}
{"id": "PMID:1092581", "title": "Electrical and secretory manifestations of glucose and amino acid interactions in rat pancreatic islets.", "content": "Interactions between glucose and amino acids in rat pancreatic islets were studied by recording the intracellular membrane potential and spike discharges from the isolated perfused pancreas.  It was found that L-isoleucine requires the presence of basal glucose (5 mM) in order to increase spike discharge from islet cells and depolarize the cell membrane.  Similarly basal glucose is needed for insulin release by L-isoleucine.  A physilolgical mixture of twenty amino acids also required the presence of basal glucose in order to increase spike activity and insulin release.  In contrast to L-isoleucine the amino acid mixture did not depolarize the beta-cells.  Iodoacetate, at concentrations previously shown to block glycolysis completely, did not interfere with any of these permissive actions of glucose, nor did iodoacetate alter the well known electrical manifestations of high levels of glucose itself (i.e. depolarization and increased spike discharge).  These data show that glucose plays a pre-eminent role as regulator of islet cell function, governing the efficacy of amino acids as beta-cells stimulants.  The results are most easily interpreted if one assumes that glycolysis is not required for glucose to exert its action.", "contents": "Electrical and secretory manifestations of glucose and amino acid interactions in rat pancreatic islets. Interactions between glucose and amino acids in rat pancreatic islets were studied by recording the intracellular membrane potential and spike discharges from the isolated perfused pancreas.  It was found that L-isoleucine requires the presence of basal glucose (5 mM) in order to increase spike discharge from islet cells and depolarize the cell membrane.  Similarly basal glucose is needed for insulin release by L-isoleucine.  A physilolgical mixture of twenty amino acids also required the presence of basal glucose in order to increase spike activity and insulin release.  In contrast to L-isoleucine the amino acid mixture did not depolarize the beta-cells.  Iodoacetate, at concentrations previously shown to block glycolysis completely, did not interfere with any of these permissive actions of glucose, nor did iodoacetate alter the well known electrical manifestations of high levels of glucose itself (i.e. depolarization and increased spike discharge).  These data show that glucose plays a pre-eminent role as regulator of islet cell function, governing the efficacy of amino acids as beta-cells stimulants.  The results are most easily interpreted if one assumes that glycolysis is not required for glucose to exert its action."}
{"id": "PMID:1092582", "title": "Pregnancy in a juvenile diabetic after renal transplantation (class T diabetes mellitus).", "content": "The successful outcome of a pregnancy in a juvenile diabetic after renal transplantation is reported.  It is proposed that class T be added to the classification of pregnancies complicated by diabetes mellitus.  Pregnancy prevention should be considered until significant longevity can be demonstrated in diabetics receiving renal transplants.", "contents": "Pregnancy in a juvenile diabetic after renal transplantation (class T diabetes mellitus). The successful outcome of a pregnancy in a juvenile diabetic after renal transplantation is reported.  It is proposed that class T be added to the classification of pregnancies complicated by diabetes mellitus.  Pregnancy prevention should be considered until significant longevity can be demonstrated in diabetics receiving renal transplants."}
{"id": "PMID:1092583", "title": "Studies of alloxan toxicity on the beta cell.", "content": "The ability of sugars to protect the beta cell  from alloxan diabetes is highly stereospecific.  The alpha anomer, which is present in equilibrium in both glucose and 3-O-methyl glucose (3-OMG) at approximately 34 per cent, provides greater protection than the beta anomer.  The greater protection of the alpha anomer of glucose is present fifteen seconds between its administration and alloxan, but there is no difference in protection following a thirty-second interval.  The nonmetabolized analogue, 3-OMG, provides even greater protection than glucose, and this higher affinity is expressed both by the lower dose necessary to provide protection, as well as by the higher dose of mannoheptulose needed to remove thr protection.  Mannoheptulose not only removes the protection provided by exogenous glucose but sensitizes the beta cell to the toxic effects of alloxan in the fasting state, probably by inhibiting the protection provided by endogenous glucose.  Mannoheptulose is able to remove glucose protection before, with, or after the administration of glucose prior to alloxan injection.  Finally, the protective effect of both glucose and 3-OMG is time-related, and the protection not only is due to absolute concentration but also appears to be affected by a changing concentration.", "contents": "Studies of alloxan toxicity on the beta cell. The ability of sugars to protect the beta cell  from alloxan diabetes is highly stereospecific.  The alpha anomer, which is present in equilibrium in both glucose and 3-O-methyl glucose (3-OMG) at approximately 34 per cent, provides greater protection than the beta anomer.  The greater protection of the alpha anomer of glucose is present fifteen seconds between its administration and alloxan, but there is no difference in protection following a thirty-second interval.  The nonmetabolized analogue, 3-OMG, provides even greater protection than glucose, and this higher affinity is expressed both by the lower dose necessary to provide protection, as well as by the higher dose of mannoheptulose needed to remove thr protection.  Mannoheptulose not only removes the protection provided by exogenous glucose but sensitizes the beta cell to the toxic effects of alloxan in the fasting state, probably by inhibiting the protection provided by endogenous glucose.  Mannoheptulose is able to remove glucose protection before, with, or after the administration of glucose prior to alloxan injection.  Finally, the protective effect of both glucose and 3-OMG is time-related, and the protection not only is due to absolute concentration but also appears to be affected by a changing concentration."}
{"id": "PMID:1092585", "title": "Comparative effects of metoclopramide and bethanechol on lower esophageal sphincter pressure in reflux patients.", "content": "The effects of oral metoclopramide, 10 and 20 mg, bethanechol, 25 mg, and placebo on lower esophageal sphincter pressure (LESP) were studied in 15 men with symptoms of gastroesophageal reflux and basal LESP less than 11 mm Hg. Each drug produced a significant increase in LESP when compared to placebo. Metoclopramide, 20 mg, produced a greater increase than either metoclopramide, 10 mg, or bethanechol, 25 mg. Serum gastrin concentrations were not altered by any of the drugs. Side effects were unremarkable. The LESP increasing effect of metoclopramide might be useful in treatment of gastro-esophageal reflux.", "contents": "Comparative effects of metoclopramide and bethanechol on lower esophageal sphincter pressure in reflux patients. The effects of oral metoclopramide, 10 and 20 mg, bethanechol, 25 mg, and placebo on lower esophageal sphincter pressure (LESP) were studied in 15 men with symptoms of gastroesophageal reflux and basal LESP less than 11 mm Hg. Each drug produced a significant increase in LESP when compared to placebo. Metoclopramide, 20 mg, produced a greater increase than either metoclopramide, 10 mg, or bethanechol, 25 mg. Serum gastrin concentrations were not altered by any of the drugs. Side effects were unremarkable. The LESP increasing effect of metoclopramide might be useful in treatment of gastro-esophageal reflux."}
{"id": "PMID:1092586", "title": "Fluorescence histochemistry of the gastrin cell infetal and adult man.", "content": "Immunoreactive gastrin cells were demonstrated in the human antral and duodenal mucosa. No gastrin immunoreactivity was seen in pancreatic islets. Formaldehyde- or Bouin-fixed material was used throughout for immunohistochemistry. Antral and duodenal gastrin cells, as well as pancreatic A2-cells, displayed strong formaldehyde- ozone- and formaldehyde-HCl-induced fluorescence, whereas the pancreatic A1-cells (claimed to store gastrin) were nonfluorescent. In the fetus, gastrin cells appeared first in the duodenal mucosa (earliest stage examined, 11 weeks) and much later in the antrum. The fluorescence histochemical properties of the fetal gastrin cells were not different from the adult.", "contents": "Fluorescence histochemistry of the gastrin cell infetal and adult man. Immunoreactive gastrin cells were demonstrated in the human antral and duodenal mucosa. No gastrin immunoreactivity was seen in pancreatic islets. Formaldehyde- or Bouin-fixed material was used throughout for immunohistochemistry. Antral and duodenal gastrin cells, as well as pancreatic A2-cells, displayed strong formaldehyde- ozone- and formaldehyde-HCl-induced fluorescence, whereas the pancreatic A1-cells (claimed to store gastrin) were nonfluorescent. In the fetus, gastrin cells appeared first in the duodenal mucosa (earliest stage examined, 11 weeks) and much later in the antrum. The fluorescence histochemical properties of the fetal gastrin cells were not different from the adult."}
{"id": "PMID:1092587", "title": "Hepatitis B surface antigen (HBS Ag) subtypes and indices of clinical disease.", "content": "Serial serum samples of prospectively studied patients exposed to plasma containing both adw and ayw subtypes of hepatitis B surface antigen (HBS Ag) were studied for the relationship of HBS Ag subtype to several indices of clinical type B hepatitis. Seventy-four patients circulated HBS Ag that could be subtyped; 50 subtype adw, 22 ayw, and 2 adyw. Although the average incubation period among adw cases was shorter than among ayw cases (P less than 0.05), no additional significant differences were detected between these two groups of patients when analyzed for severity and chronicity of disease. The chronic HBS Ag carrier state developed in 16% (8 of 50) adw cases and in 9% (2 of 22) ayw cases, but this difference was not statistically significant. Judging from these data, it was concluded that the severity and outcome of type B hepatitis are probably independent of HBS Ag subtypes d and y.", "contents": "Hepatitis B surface antigen (HBS Ag) subtypes and indices of clinical disease. Serial serum samples of prospectively studied patients exposed to plasma containing both adw and ayw subtypes of hepatitis B surface antigen (HBS Ag) were studied for the relationship of HBS Ag subtype to several indices of clinical type B hepatitis. Seventy-four patients circulated HBS Ag that could be subtyped; 50 subtype adw, 22 ayw, and 2 adyw. Although the average incubation period among adw cases was shorter than among ayw cases (P less than 0.05), no additional significant differences were detected between these two groups of patients when analyzed for severity and chronicity of disease. The chronic HBS Ag carrier state developed in 16% (8 of 50) adw cases and in 9% (2 of 22) ayw cases, but this difference was not statistically significant. Judging from these data, it was concluded that the severity and outcome of type B hepatitis are probably independent of HBS Ag subtypes d and y."}
{"id": "PMID:1092589", "title": "Colonoscopy. A review.", "content": "The technique of colonoscopy has introduced an exciting new modality for the diagnosis and treatment of diseases of the colon. It is not a panacrea, for, as with any technique, limitations are always present; but it offers the single most far reaching advance for the diagnosis and treatment of diseases of the colon that has been offered in the last decade. The next 10 years should indeed prove exciting.", "contents": "Colonoscopy. A review. The technique of colonoscopy has introduced an exciting new modality for the diagnosis and treatment of diseases of the colon. It is not a panacrea, for, as with any technique, limitations are always present; but it offers the single most far reaching advance for the diagnosis and treatment of diseases of the colon that has been offered in the last decade. The next 10 years should indeed prove exciting."}
{"id": "PMID:1092592", "title": "Action of morphine sulfphate on stimulated gastric secretion in dogs.", "content": "In dogs morphine sulphate suppresses both 2-deoxy-D-glucose and pentagastrin-stimulated acid and pepsin secretion, but not histimaine- and pilocarpine-stimulated acid. This is consistent with the view that morphine both depresses acetylcholine release from cholinergic endings in the stomach and that pentagastrin acts by liberation of acetylcholine from these endings.", "contents": "Action of morphine sulfphate on stimulated gastric secretion in dogs. In dogs morphine sulphate suppresses both 2-deoxy-D-glucose and pentagastrin-stimulated acid and pepsin secretion, but not histimaine- and pilocarpine-stimulated acid. This is consistent with the view that morphine both depresses acetylcholine release from cholinergic endings in the stomach and that pentagastrin acts by liberation of acetylcholine from these endings."}
{"id": "PMID:1092593", "title": "[Single dose treatment of trichomoniasis].", "content": "The present regiman for the treatment of trichomoniasis with Tinidazol is 150 mg. twice daily for 7 days with a total dose of 2,100 mg. The success rate of this regimen is 85-90%. With a single dosage treatment of 2,000 mg. (Four 500 mg. tablets for the patient and her sexual partner) the success rate improved to 100%. Toxic side effects were not observed. The treatment was well tolerated and well accepted.", "contents": "[Single dose treatment of trichomoniasis]. The present regiman for the treatment of trichomoniasis with Tinidazol is 150 mg. twice daily for 7 days with a total dose of 2,100 mg. The success rate of this regimen is 85-90%. With a single dosage treatment of 2,000 mg. (Four 500 mg. tablets for the patient and her sexual partner) the success rate improved to 100%. Toxic side effects were not observed. The treatment was well tolerated and well accepted."}
{"id": "PMID:1092594", "title": "[Present situation of prenatal diagnosis of neural tube defects].", "content": "Anencephaly and spina bifida both are malformations of unknown etiology which are caused by an absent or incomplete closure of the neural tube. The risk of recurrence for the malformations is 5% in families with one child with a neural tube defect and 10%, if two such children have already been born. Neural tube defects can be diagnosed already before 20th week of gestation by ultrasonics and alpha-fetoprotein measurement in amniotic fluid, if an underdeveloped neurocranium or a free communication between amniotic and cerebrospinal fluid is present. By these diagnostic techniques and possibly following interruption the probability in risk families can be significantly lowered.", "contents": "[Present situation of prenatal diagnosis of neural tube defects]. Anencephaly and spina bifida both are malformations of unknown etiology which are caused by an absent or incomplete closure of the neural tube. The risk of recurrence for the malformations is 5% in families with one child with a neural tube defect and 10%, if two such children have already been born. Neural tube defects can be diagnosed already before 20th week of gestation by ultrasonics and alpha-fetoprotein measurement in amniotic fluid, if an underdeveloped neurocranium or a free communication between amniotic and cerebrospinal fluid is present. By these diagnostic techniques and possibly following interruption the probability in risk families can be significantly lowered."}
{"id": "PMID:1092596", "title": "Reciprocal translocations and translocation disomics of aspergillus and their use for genetic mapping.", "content": "Two new techniques are described for genetic mapping of reciprocal translocations in A. nidulans, which can be used to locate centromeres and meiotically unlinked markers. They both make use of unbalanced disomics from heterozygous translocation crosses. These are mainly hyperhaploids of two classes: either typical-looking n + 1 with a normal chromosome in addition to a haploid set containing the translocation, or translocation disomics. When large chromosome segments are involved, such disomics, as well as stable aneuploids and duplication types, show characteristic phenotypes and can be classified visually. The first method maps translocation breaks qualitatively, since translocated markers can be identified when translocation disomics are analyzed for heterozygous markers. The second method measures meiotic linkage of any marker to the translocation breaks when allele ratios in the balanced haploid sectors of either or both classes of disomics are determined: linked markers show reciprocal deviations from 1:1--In addition, it can be shown that frequencies of nondisjunction and recovery of specific translocation disomics both depend on the relative position of the break within a chromosome arm. Such information can provide a rough estimate of the positions of breaks for a new translocation.--Using these techniques, as well as mitotic mapping in homo- and heterozygous translocation diploids, four reciprocal translocations were mapped. from these results, information on the sequence and orientation of most of the \"meiotic fragments\" of the current maps (groups III, VI, VII and VIII) was obtained, and the position of the centromeres of groups VI and VII were identified. Translocation disomics are also used to map meiotically unlinked single genes, e.g. oliA of group VII, to specify chromosome segments.", "contents": "Reciprocal translocations and translocation disomics of aspergillus and their use for genetic mapping. Two new techniques are described for genetic mapping of reciprocal translocations in A. nidulans, which can be used to locate centromeres and meiotically unlinked markers. They both make use of unbalanced disomics from heterozygous translocation crosses. These are mainly hyperhaploids of two classes: either typical-looking n + 1 with a normal chromosome in addition to a haploid set containing the translocation, or translocation disomics. When large chromosome segments are involved, such disomics, as well as stable aneuploids and duplication types, show characteristic phenotypes and can be classified visually. The first method maps translocation breaks qualitatively, since translocated markers can be identified when translocation disomics are analyzed for heterozygous markers. The second method measures meiotic linkage of any marker to the translocation breaks when allele ratios in the balanced haploid sectors of either or both classes of disomics are determined: linked markers show reciprocal deviations from 1:1--In addition, it can be shown that frequencies of nondisjunction and recovery of specific translocation disomics both depend on the relative position of the break within a chromosome arm. Such information can provide a rough estimate of the positions of breaks for a new translocation.--Using these techniques, as well as mitotic mapping in homo- and heterozygous translocation diploids, four reciprocal translocations were mapped. from these results, information on the sequence and orientation of most of the \"meiotic fragments\" of the current maps (groups III, VI, VII and VIII) was obtained, and the position of the centromeres of groups VI and VII were identified. Translocation disomics are also used to map meiotically unlinked single genes, e.g. oliA of group VII, to specify chromosome segments."}
{"id": "PMID:1092597", "title": "Mitotic chromosome loss in a disomic haploid of Saccharomyces cerevisiae.", "content": "Experiments designed to characterize the incidence of mitotic chromosome loss in a yeast disomic haploid were performed; The selective methods employed utulize the non-mating property of strains disomic for linkage group III and heterozygous at the mating type locus. The principal findings are: (1) The grequency of spontaneous chromosome loss in the disome is of the order 10- minus 4 per cell; this value approximates the frequency in the same population of spontaneous mitotic exchange resulting in homozygosity at the mating type locus. (2) The recovered diploids are pure clones, and thus represent unique events in the disomic haploid. (3) Of the euploid chromosomes recovered after events leading to chromosome loss, approximately 90% retain the parental marker configuration expected from segregation alone; however, the remainder are recombinant for marker genes, and are the result of mitotic exchanges in the disome, especially in regions near the centromere. The recombinant proportion significantly exceeds that expected if chromosome loss and mitotic exchange in the disome were independent events. The data are consistent with a model proposing mitotic nondisjunction as the event responsible for chromosome loss in the disomic haploid.", "contents": "Mitotic chromosome loss in a disomic haploid of Saccharomyces cerevisiae. Experiments designed to characterize the incidence of mitotic chromosome loss in a yeast disomic haploid were performed; The selective methods employed utulize the non-mating property of strains disomic for linkage group III and heterozygous at the mating type locus. The principal findings are: (1) The grequency of spontaneous chromosome loss in the disome is of the order 10- minus 4 per cell; this value approximates the frequency in the same population of spontaneous mitotic exchange resulting in homozygosity at the mating type locus. (2) The recovered diploids are pure clones, and thus represent unique events in the disomic haploid. (3) Of the euploid chromosomes recovered after events leading to chromosome loss, approximately 90% retain the parental marker configuration expected from segregation alone; however, the remainder are recombinant for marker genes, and are the result of mitotic exchanges in the disome, especially in regions near the centromere. The recombinant proportion significantly exceeds that expected if chromosome loss and mitotic exchange in the disome were independent events. The data are consistent with a model proposing mitotic nondisjunction as the event responsible for chromosome loss in the disomic haploid."}
{"id": "PMID:1092601", "title": "The distribution of mucosal antibodies in the bowel of patients with Crohn's disease.", "content": "Specimens of intestine from 24 patients with histologically proven Crohn's disease have been studies by an immunofluorescent technique for the distribution of immunoglobulins A, G, M, E, and D. A marked reduction of IgA in lymphoid and epithelial cells was noted in those areas of the bowel which, although histologically affected, were not ulcerated; in the histologically unaffected areas of the bowel the distribution of IgA was similar to that found in control sections of normal bowel. There was an increase in IG7 in the affected areas but the numbers of lymphoid cells staining for IgD did not differ from those found in normal controls; in 12 cases of Crohn's disease, however, IgG appeared to be present in bound form on the surface of, and in, the epithelial cells. It is postulated that the local deficiency in IgA in the affected areas may be secondary to a local cell-mediated immune reaction, to a focal metaplasia of the surface epithelium, or to patchy bindings of anticolon antibodies to the surface epithelium. The effect of this local deficiency of IgA will be to allow for an increased focal entry of antigenic material into the bowel wall, and it is suggested that many of the pathological features of Crohn's disease are explicable on the basis of deep penetration of a variety of antigens.", "contents": "The distribution of mucosal antibodies in the bowel of patients with Crohn's disease. Specimens of intestine from 24 patients with histologically proven Crohn's disease have been studies by an immunofluorescent technique for the distribution of immunoglobulins A, G, M, E, and D. A marked reduction of IgA in lymphoid and epithelial cells was noted in those areas of the bowel which, although histologically affected, were not ulcerated; in the histologically unaffected areas of the bowel the distribution of IgA was similar to that found in control sections of normal bowel. There was an increase in IG7 in the affected areas but the numbers of lymphoid cells staining for IgD did not differ from those found in normal controls; in 12 cases of Crohn's disease, however, IgG appeared to be present in bound form on the surface of, and in, the epithelial cells. It is postulated that the local deficiency in IgA in the affected areas may be secondary to a local cell-mediated immune reaction, to a focal metaplasia of the surface epithelium, or to patchy bindings of anticolon antibodies to the surface epithelium. The effect of this local deficiency of IgA will be to allow for an increased focal entry of antigenic material into the bowel wall, and it is suggested that many of the pathological features of Crohn's disease are explicable on the basis of deep penetration of a variety of antigens."}
{"id": "PMID:1092602", "title": "Influence of exocrine and endocrine pancreatic function on intestinal brush border enaymatic activities.", "content": "Digestive enzymatic activities (disaccharidases, alkaline phosphatase, peptide hydrolases) have been determined in the mucosa of 14 patients with chronic pancreatitis. All had an abnormal secretin-pancreozymin test. Four patients had insulin-dependent diabetes mellitus, four a pathological glucose tolerance test. Nine patients had steatorrhoea. Maltase, sucrase, and alkaline phosphatase activity was significantly elevated in patients with exocrine pancreatic insufficiency, whereas those of lactase, trehalase, and peptide hydrolase were normal. Patients with steatorrhoea had higher maltase and sucrase activity than those without steatorrhoea, whereas decreased glucose tolerance had no effect on brush border enzymatic activity. It is suggested thatdecreased exocrine rather than decreased endocrine pancreatic function is responsible for the increase in intestinal disaccharidase and alkaline phosphatase activity, possible by the influence of pacreatic enzymes on the turnover of brush border enzymes from the luminal side of the mucosal membranes or by direct hormonal stimulation though cholecystokinin.", "contents": "Influence of exocrine and endocrine pancreatic function on intestinal brush border enaymatic activities. Digestive enzymatic activities (disaccharidases, alkaline phosphatase, peptide hydrolases) have been determined in the mucosa of 14 patients with chronic pancreatitis. All had an abnormal secretin-pancreozymin test. Four patients had insulin-dependent diabetes mellitus, four a pathological glucose tolerance test. Nine patients had steatorrhoea. Maltase, sucrase, and alkaline phosphatase activity was significantly elevated in patients with exocrine pancreatic insufficiency, whereas those of lactase, trehalase, and peptide hydrolase were normal. Patients with steatorrhoea had higher maltase and sucrase activity than those without steatorrhoea, whereas decreased glucose tolerance had no effect on brush border enzymatic activity. It is suggested thatdecreased exocrine rather than decreased endocrine pancreatic function is responsible for the increase in intestinal disaccharidase and alkaline phosphatase activity, possible by the influence of pacreatic enzymes on the turnover of brush border enzymes from the luminal side of the mucosal membranes or by direct hormonal stimulation though cholecystokinin."}
{"id": "PMID:1092608", "title": "Release of 14C label and complement killing of Escherichia coli.", "content": "When Escherichia coli labelled with 14C were killed by complement, characteristic 14C compounds were released even when complete cell disintegration was prevented or delayed by removal of lysozyme. Treatment with heated serum only resulted in the loss of small molecular weight compounds. Separation of the products was made easier if whole serum was replaced by a salt-precipitated fraction which contained no albumin or lysozyme but retained antibody and complement. Fractionation of the bacterial products on Sephadex G-200 showed two radioactive peaks containing lipids and proteins of which a preliminary examination was made. The release of these compounds was related to complement action, since they were not found when bacteria were killed by streptomycin or ultra-violet light in the absence of complement. Nor were they found when resistant bacteria were treated with complement and survived. The possible modes of action of complement on bacterial cell walls are discussed.", "contents": "Release of 14C label and complement killing of Escherichia coli. When Escherichia coli labelled with 14C were killed by complement, characteristic 14C compounds were released even when complete cell disintegration was prevented or delayed by removal of lysozyme. Treatment with heated serum only resulted in the loss of small molecular weight compounds. Separation of the products was made easier if whole serum was replaced by a salt-precipitated fraction which contained no albumin or lysozyme but retained antibody and complement. Fractionation of the bacterial products on Sephadex G-200 showed two radioactive peaks containing lipids and proteins of which a preliminary examination was made. The release of these compounds was related to complement action, since they were not found when bacteria were killed by streptomycin or ultra-violet light in the absence of complement. Nor were they found when resistant bacteria were treated with complement and survived. The possible modes of action of complement on bacterial cell walls are discussed."}
{"id": "PMID:1092606", "title": "Comparison of methods for the detection of Mycoplasmal contamination of cell cultures: a review.", "content": "Several reviews in recent years have emphasized the problems created by mycoplasmal contamination of cultured cells (1-5). Because of the hazards of interpreting data derived from mycoplasma contaminated cells, most cell biologists routinely screen their cultures for the presence of these organisms. In recent years, the limitations of standard microbiological testing for mycoplasmas have become increasingly apparent and have led to the development of several new biochemical techniques for detection of these organisms. The aim of this review is to describe and compare available detection techniques and to evaluate their relative efficacy. Those properties of mycoplasmas that are relevant to their role as cell culture contaminants will be briefly discussed.", "contents": "Comparison of methods for the detection of Mycoplasmal contamination of cell cultures: a review. Several reviews in recent years have emphasized the problems created by mycoplasmal contamination of cultured cells (1-5). Because of the hazards of interpreting data derived from mycoplasma contaminated cells, most cell biologists routinely screen their cultures for the presence of these organisms. In recent years, the limitations of standard microbiological testing for mycoplasmas have become increasingly apparent and have led to the development of several new biochemical techniques for detection of these organisms. The aim of this review is to describe and compare available detection techniques and to evaluate their relative efficacy. Those properties of mycoplasmas that are relevant to their role as cell culture contaminants will be briefly discussed."}
{"id": "PMID:1092609", "title": "Effect of Corynebacterium parvum on the class and subclass of antibody produced in the response of different strains of mice to sheep erythrocytes.", "content": "Several strains of mice were injected with sheep erythrocytes (SRBC) using C. parvum as adjuvant. The adjuvant effects on the amounts of class and subclass of antibody produced were ranked in the order IgG2b greater than IgG2a and IgM greater than IgG1. In addition, these effects were shown to vary depending on the time of administration of C. parvum relative to antigen. C parvum was shown to have no adjuvant effect on the response of congenitally athymic mice when given at the same time as the antigen, SRBC. On the basis of the reported observations it is suggested that certain of the adjuvant effects of C. parvum require T-cell function.", "contents": "Effect of Corynebacterium parvum on the class and subclass of antibody produced in the response of different strains of mice to sheep erythrocytes. Several strains of mice were injected with sheep erythrocytes (SRBC) using C. parvum as adjuvant. The adjuvant effects on the amounts of class and subclass of antibody produced were ranked in the order IgG2b greater than IgG2a and IgM greater than IgG1. In addition, these effects were shown to vary depending on the time of administration of C. parvum relative to antigen. C parvum was shown to have no adjuvant effect on the response of congenitally athymic mice when given at the same time as the antigen, SRBC. On the basis of the reported observations it is suggested that certain of the adjuvant effects of C. parvum require T-cell function."}
{"id": "PMID:1092607", "title": "Hydrolysis of oligopeptides by sera used in cell and tissue culture.", "content": "Sera commonly used in cell and tissue culture as medium supplements possess high peptidase activity. Pligopeptides incubated with 1% serum are rapidly hydrolyzed to intermediate length peptides and the constituent amino acids. Hydrolysis of lysine peptides is difficult to verify by a quantitative ninhydrin procedure because of reaction of the lysine epsi-lon-amino group and serum components with ninhydrin. Attempts to evaluate oligopeptides as sole sources of indispensable amino acids for cultured mammalian cells are of doubtful value when serum is used as a medium supplement.", "contents": "Hydrolysis of oligopeptides by sera used in cell and tissue culture. Sera commonly used in cell and tissue culture as medium supplements possess high peptidase activity. Pligopeptides incubated with 1% serum are rapidly hydrolyzed to intermediate length peptides and the constituent amino acids. Hydrolysis of lysine peptides is difficult to verify by a quantitative ninhydrin procedure because of reaction of the lysine epsi-lon-amino group and serum components with ninhydrin. Attempts to evaluate oligopeptides as sole sources of indispensable amino acids for cultured mammalian cells are of doubtful value when serum is used as a medium supplement."}
{"id": "PMID:1092610", "title": "Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation.", "content": "A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its time of administration in realtion to antigen. Double-stranded RNA administered with antigen, or a few hours after antigen, produced a variable degree of enhancement of plaque-forming cell numbers or agglutinating antibody levels depending on the antigen involved. Administration of double-stranded RNA 24 hours before antigen invariably produced a suppressed response. In mice which were either specifically hyporesponsive (tolerant) or non-specifically hyporesponsive (due to age or immunosuppressive drugs) double-stranded RNA administered with antigen resulted in a nearly normal immune response.", "contents": "Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation. A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its time of administration in realtion to antigen. Double-stranded RNA administered with antigen, or a few hours after antigen, produced a variable degree of enhancement of plaque-forming cell numbers or agglutinating antibody levels depending on the antigen involved. Administration of double-stranded RNA 24 hours before antigen invariably produced a suppressed response. In mice which were either specifically hyporesponsive (tolerant) or non-specifically hyporesponsive (due to age or immunosuppressive drugs) double-stranded RNA administered with antigen resulted in a nearly normal immune response."}
{"id": "PMID:1092611", "title": "T cell-dependent helper and suppressive influences in an adoptive IgG antibody response.", "content": "When immune spleen cells of mice immunized to a hapten carrier preparation 4 days previously were transferred to normal syngeneic hosts, they began to produce 7S antibody (presumably of the IgG class), provided that relatively small numbers of cells (about 1/10 spleen equivalent) were transferred. Increasing the number of transferred cells resulted in less IgG antibody formed. Depletion of the immune spleen cells of T cells by treatment with anti-theta serum and complement prevented IgG antibody formation. IgG antibody production by untreated and anti-therta serum-treated immune spleen cells could be enhanced (reinduced) by addition of small numbers of cells enriched for carrier-activated T cells. These suggest that T cells are necessary to stimulate antigen-activated B cells into IgG antibody production. Larger numbers of 'carrier-activated T cells' depressed IgG antibody production. Both enhancement and depression could be demonstrated to be antigen-specific. IgG antibody production high numbers of transferred immune spleen cells could be induced by treating the cells prior to transfer with suboptimal amounts of anti-theta serum and complement. It is argued that this results from the elimination of a T cell-dependent suppressor influence arising during a normal immune response.", "contents": "T cell-dependent helper and suppressive influences in an adoptive IgG antibody response. When immune spleen cells of mice immunized to a hapten carrier preparation 4 days previously were transferred to normal syngeneic hosts, they began to produce 7S antibody (presumably of the IgG class), provided that relatively small numbers of cells (about 1/10 spleen equivalent) were transferred. Increasing the number of transferred cells resulted in less IgG antibody formed. Depletion of the immune spleen cells of T cells by treatment with anti-theta serum and complement prevented IgG antibody formation. IgG antibody production by untreated and anti-therta serum-treated immune spleen cells could be enhanced (reinduced) by addition of small numbers of cells enriched for carrier-activated T cells. These suggest that T cells are necessary to stimulate antigen-activated B cells into IgG antibody production. Larger numbers of 'carrier-activated T cells' depressed IgG antibody production. Both enhancement and depression could be demonstrated to be antigen-specific. IgG antibody production high numbers of transferred immune spleen cells could be induced by treating the cells prior to transfer with suboptimal amounts of anti-theta serum and complement. It is argued that this results from the elimination of a T cell-dependent suppressor influence arising during a normal immune response."}
{"id": "PMID:1092612", "title": "T cell-dependent mediator in the immune response. III. The role of non-specific factor (NSF) in the in vitro immune response.", "content": "Evidence is presented which suggests that T cell-dependent nonspecific factor (NSF) cannot be the sole mediator of 'co-operation' for all types of thymus-dependent antibody response. NSF can replace the requirement for T cells in responses to hapten conjugated to particulate carriers, but cannot do so in responses to hapten conjugates of soluble thymus-dependent carriers.", "contents": "T cell-dependent mediator in the immune response. III. The role of non-specific factor (NSF) in the in vitro immune response. Evidence is presented which suggests that T cell-dependent nonspecific factor (NSF) cannot be the sole mediator of 'co-operation' for all types of thymus-dependent antibody response. NSF can replace the requirement for T cells in responses to hapten conjugated to particulate carriers, but cannot do so in responses to hapten conjugates of soluble thymus-dependent carriers."}
{"id": "PMID:1092613", "title": "The inter-relationship of antigenic structure, thymus-independence and adjuvanticity. IV. A general model for B-cell induction.", "content": "Polymerized flagellin and E. coli lipopolysaccharide both express adjuvanticity in vivo and in vitro for responses to hapten conjugated to sheep erythrocytes, and hapten conjugated to soluble thymus-dependent antigens. In the case of erythrocyte-bound hapten, adjuvanticity is expressed in the absence of thymus-derived cells (T cells). However, in the case of responses to soluble thymus-dependent conjugates, carrier-specific T cells would appear to be necessary for adjuvanticity to be expressed. On the basis of these observations an hypothesis for the mechanism of B-cell induction and tolerance is proposed.", "contents": "The inter-relationship of antigenic structure, thymus-independence and adjuvanticity. IV. A general model for B-cell induction. Polymerized flagellin and E. coli lipopolysaccharide both express adjuvanticity in vivo and in vitro for responses to hapten conjugated to sheep erythrocytes, and hapten conjugated to soluble thymus-dependent antigens. In the case of erythrocyte-bound hapten, adjuvanticity is expressed in the absence of thymus-derived cells (T cells). However, in the case of responses to soluble thymus-dependent conjugates, carrier-specific T cells would appear to be necessary for adjuvanticity to be expressed. On the basis of these observations an hypothesis for the mechanism of B-cell induction and tolerance is proposed."}
{"id": "PMID:1092614", "title": "C3 receptors on direct plaque-forming cells.", "content": "Using a rosette technique it is shown that only a small proportion of direct plaque-forming cells posses detectable C3 receptors 5 and 7 days after antigenic stimulation. The significance of this result is discussed.", "contents": "C3 receptors on direct plaque-forming cells. Using a rosette technique it is shown that only a small proportion of direct plaque-forming cells posses detectable C3 receptors 5 and 7 days after antigenic stimulation. The significance of this result is discussed."}
{"id": "PMID:1092615", "title": "A series of tibial fractures treated conservatively.", "content": "Two hundred and two fractures of the tibia have been reviewed. They were treated by a standard, conservative method; with a Phemister graft when union was delayed and a pseudarthrosis threatened. The classifications of Charnley (1968) and Nicoll (1964) have proved to be useful in assessing results and these complement each other. The factors that influence bony union include damage to the interosseous membrane, the degree of the displacement and comminution of the fracture, and injury to soft tissues. Infection has a very unfavourable influence on bone union and leads to the majority of bad sequelae. The fundamental object in reduction is to obtain as perfect apposition and alignment of the fragments as possible. Imperfect apposition, however, with normal alignment does not affect bone union severely. The problem arising from the use of an above-knee walking plaster that affects the patient physically, psychologically and socially, may be solved by the early use of a below-knee plaster. The functional sequelae are the result of soft-tissue damage rather than of immobilization. The Chutro-Phemister type of graft has proved to be a safe method in preventing pseudarthrosis. The analysis of the results obtained with this conservative method is sufficient reason against choosing surgical treatment for these fractures.", "contents": "A series of tibial fractures treated conservatively. Two hundred and two fractures of the tibia have been reviewed. They were treated by a standard, conservative method; with a Phemister graft when union was delayed and a pseudarthrosis threatened. The classifications of Charnley (1968) and Nicoll (1964) have proved to be useful in assessing results and these complement each other. The factors that influence bony union include damage to the interosseous membrane, the degree of the displacement and comminution of the fracture, and injury to soft tissues. Infection has a very unfavourable influence on bone union and leads to the majority of bad sequelae. The fundamental object in reduction is to obtain as perfect apposition and alignment of the fragments as possible. Imperfect apposition, however, with normal alignment does not affect bone union severely. The problem arising from the use of an above-knee walking plaster that affects the patient physically, psychologically and socially, may be solved by the early use of a below-knee plaster. The functional sequelae are the result of soft-tissue damage rather than of immobilization. The Chutro-Phemister type of graft has proved to be a safe method in preventing pseudarthrosis. The analysis of the results obtained with this conservative method is sufficient reason against choosing surgical treatment for these fractures."}
{"id": "PMID:1092616", "title": "The place and efficacy of operative management of fractured clavicle.", "content": "A study of 22 cases of operative fixation of the fractured clavicle has been made. This has shown that with internal fixation and onlay bone grafting, highly successful results can be attained. There were no cases of non-union and there have been no significant complications. The indications for such surgery are presented from the literature. It is clear that the operation is not commonly indicated, but when performed is safe and highly successful.", "contents": "The place and efficacy of operative management of fractured clavicle. A study of 22 cases of operative fixation of the fractured clavicle has been made. This has shown that with internal fixation and onlay bone grafting, highly successful results can be attained. There were no cases of non-union and there have been no significant complications. The indications for such surgery are presented from the literature. It is clear that the operation is not commonly indicated, but when performed is safe and highly successful."}
{"id": "PMID:1092617", "title": "Prophylaxis with dextran 70 against thrombosis in patients with fractures of the upper end of the femur.", "content": "A trial was undertaken to assess the effect of dextran 70 (Macrodex) in prophylaxis against thrombosis in womem over 65 years with fractures of the upper end of the femur. The dosage used was 500 ml at operation and 500 ml on the first postoperative day. At this dosage given at this time there was no difference in the overall incidence of clinically diagnosed deep venous thrombosis in the group of patients given dextran 70 compared with the control group. There was a tendency for thrombosis to occur earlier in the control group. The mortality rates at 3 weeks and even at 6 months were less in the treated group than in the control series.", "contents": "Prophylaxis with dextran 70 against thrombosis in patients with fractures of the upper end of the femur. A trial was undertaken to assess the effect of dextran 70 (Macrodex) in prophylaxis against thrombosis in womem over 65 years with fractures of the upper end of the femur. The dosage used was 500 ml at operation and 500 ml on the first postoperative day. At this dosage given at this time there was no difference in the overall incidence of clinically diagnosed deep venous thrombosis in the group of patients given dextran 70 compared with the control group. There was a tendency for thrombosis to occur earlier in the control group. The mortality rates at 3 weeks and even at 6 months were less in the treated group than in the control series."}
{"id": "PMID:1092620", "title": "Oncogenesis by interspecific interaction of malignant murine and non-malignant hamster cells in vitro.", "content": "A clone of Cloudman S91 murine melanoma was fused in vitro with non-malignant hamster cheek pouch cells by means of lysolecithin, and the putative hybrid progeny cells, HCP-MM, were found to be highly malignant in hamster, but not in appropriate mice. A malignant clone of HCP-MM cells was shown to have hamster species-specific surface antigens (as demonstrated by immunofluorescence and the cytotoxic antibody) and hamster-like lactate dehydrogenase and NAD-dependent malate dehydrogenase isoenzyme profiles. Nevertheless, chromosomes similar to those of both murine and hamster parental cells could be distinguished in cells of this malignant clone and in hamster tumor grafts by the method of trypsin-Giemsa banding. A majority of the murine chromosomes, however, appeared to be lost. This study indicates that a murine melanoma previously found untransplantable in hamsters could produce a highly malignant and lethal tumor for hamsters after being mixed in vitro with non-malignant hamster cells, in the presence of a fusing chemical. It is not as yet certain whether the production of transformed cells in vitro and of highly malignant tumors in the hamster (both with predominantly hamster properties) required heterosynkarion formation between the murine melanoma and hamster cheek pouch cells. Nevertheless, our results suggest that the presence of the murine melanoma, and possibly the interaction of its genome with non-malignant hamster cells, was implicated in this process.", "contents": "Oncogenesis by interspecific interaction of malignant murine and non-malignant hamster cells in vitro. A clone of Cloudman S91 murine melanoma was fused in vitro with non-malignant hamster cheek pouch cells by means of lysolecithin, and the putative hybrid progeny cells, HCP-MM, were found to be highly malignant in hamster, but not in appropriate mice. A malignant clone of HCP-MM cells was shown to have hamster species-specific surface antigens (as demonstrated by immunofluorescence and the cytotoxic antibody) and hamster-like lactate dehydrogenase and NAD-dependent malate dehydrogenase isoenzyme profiles. Nevertheless, chromosomes similar to those of both murine and hamster parental cells could be distinguished in cells of this malignant clone and in hamster tumor grafts by the method of trypsin-Giemsa banding. A majority of the murine chromosomes, however, appeared to be lost. This study indicates that a murine melanoma previously found untransplantable in hamsters could produce a highly malignant and lethal tumor for hamsters after being mixed in vitro with non-malignant hamster cells, in the presence of a fusing chemical. It is not as yet certain whether the production of transformed cells in vitro and of highly malignant tumors in the hamster (both with predominantly hamster properties) required heterosynkarion formation between the murine melanoma and hamster cheek pouch cells. Nevertheless, our results suggest that the presence of the murine melanoma, and possibly the interaction of its genome with non-malignant hamster cells, was implicated in this process."}
{"id": "PMID:1092627", "title": "Mycetoma in Iran.", "content": "The subject of mycetoma is reviewed and 7 cases are reported for the first time from southern Iran.  Four were causes by Actinomyces israelii and one each by Nocardia asteroides, Actinomadura madurae and Allescheria boydii.", "contents": "Mycetoma in Iran. The subject of mycetoma is reviewed and 7 cases are reported for the first time from southern Iran.  Four were causes by Actinomyces israelii and one each by Nocardia asteroides, Actinomadura madurae and Allescheria boydii."}
{"id": "PMID:1092631", "title": "Silver sulfadiazine for control of burn wound infections.", "content": "Silver sulfadiazine (Silvadene, U.S.; Flamazine, U.K.; Flammazine, N.; Sulplata, S.A.) is the newest topical antimicrobial agent available following worldwide clinical trials. Good control of infection is achieved without pain or other demonstrable side effects, using either dressings or the exposure technic. Many burned areas kept free of infection heal without grafting. Where necessary, early preparation for and good take of grafts has been attained by utilizing this new therapeutic agent. Markedly reduced mortality from burn wound sepsis has generally been observed.", "contents": "Silver sulfadiazine for control of burn wound infections. Silver sulfadiazine (Silvadene, U.S.; Flamazine, U.K.; Flammazine, N.; Sulplata, S.A.) is the newest topical antimicrobial agent available following worldwide clinical trials. Good control of infection is achieved without pain or other demonstrable side effects, using either dressings or the exposure technic. Many burned areas kept free of infection heal without grafting. Where necessary, early preparation for and good take of grafts has been attained by utilizing this new therapeutic agent. Markedly reduced mortality from burn wound sepsis has generally been observed."}
{"id": "PMID:1092636", "title": "Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.", "content": "Out of a total of eleven bifunctional reagents tested as fixatives for light microscopic immunohistochemistry, four were found satisfactory when applied in the vapour phase to freeze-dried blocks. These were diethylpyrocarbonate, as observed in earlier studies, dimethyladipimidate, p-benzoquinone, and diacetyl. Adequate but less satisfactory liquid-phase fixation was provided by three reagents (dimethyladipimidate, dimethylsuberimidate, p-benzoquinone). None of the eleven reagents gave optimal preservation of antigencity and structure when tested at the ultrastructural level. Encouraging results were obtained with p-benzoquinone, however.", "contents": "Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry. Out of a total of eleven bifunctional reagents tested as fixatives for light microscopic immunohistochemistry, four were found satisfactory when applied in the vapour phase to freeze-dried blocks. These were diethylpyrocarbonate, as observed in earlier studies, dimethyladipimidate, p-benzoquinone, and diacetyl. Adequate but less satisfactory liquid-phase fixation was provided by three reagents (dimethyladipimidate, dimethylsuberimidate, p-benzoquinone). None of the eleven reagents gave optimal preservation of antigencity and structure when tested at the ultrastructural level. Encouraging results were obtained with p-benzoquinone, however."}
{"id": "PMID:1092637", "title": "Immunofluorescent localization of the female sex steroids in the porcine ovary.", "content": "Antibodies against progesterone 20-(o-carboxymethyl) oxime and oestrone 17-(o-carboxymethyl) oxime have been applied to porcine ovaries as the first layer in the indirect immunofluorescent technique. In the Graafian follicles, both steroids have been demonstrated within single cells of the theca interna. In the corpora lutea, the same steroids were found in the cells assembled at the periphery of the organ, as well as around the large vessels. Degenerating cells present in the corpora albicans revealed positive immunofluorescence together with distinct autofluorescence. Similarly, the atretic follicles and fragments of the interstitial gland showed both immunofluorescence and autofluorescence. The immunofluorescent localization of steroids was confirmed by the similar distribution of sudanophilic substances. However, the differentiation between the two hormones investigated was not certain.", "contents": "Immunofluorescent localization of the female sex steroids in the porcine ovary. Antibodies against progesterone 20-(o-carboxymethyl) oxime and oestrone 17-(o-carboxymethyl) oxime have been applied to porcine ovaries as the first layer in the indirect immunofluorescent technique. In the Graafian follicles, both steroids have been demonstrated within single cells of the theca interna. In the corpora lutea, the same steroids were found in the cells assembled at the periphery of the organ, as well as around the large vessels. Degenerating cells present in the corpora albicans revealed positive immunofluorescence together with distinct autofluorescence. Similarly, the atretic follicles and fragments of the interstitial gland showed both immunofluorescence and autofluorescence. The immunofluorescent localization of steroids was confirmed by the similar distribution of sudanophilic substances. However, the differentiation between the two hormones investigated was not certain."}
{"id": "PMID:1092638", "title": "The gentamicin antibiotics. 6. Gentamicin C2b, an aminoglycoside antibiotic produced by Micromonospora purpurea mutant JI-33.", "content": "A mutant strain of Micromonospora purpurea, designated var. JI-33, produced an antibiotic complex consisting primarily of gentamicin C1a. A further product of this fermentation was identical to a very minor component isolated from the fermentation of the parent organism and named gentamicin C2b. Physical measurements indicated its structure to be 6'-N-methylgentamicin C1a and this was confirmed by synthesis from gentamicin C1a. The in vitro antibacterial activity of gentamicin C2b was very similar to that of the gentamicin C complex. Antibiotic XK-62-2, produced by Micromonospora sagamiensis, appears to be identical to gentamicin C1b.", "contents": "The gentamicin antibiotics. 6. Gentamicin C2b, an aminoglycoside antibiotic produced by Micromonospora purpurea mutant JI-33. A mutant strain of Micromonospora purpurea, designated var. JI-33, produced an antibiotic complex consisting primarily of gentamicin C1a. A further product of this fermentation was identical to a very minor component isolated from the fermentation of the parent organism and named gentamicin C2b. Physical measurements indicated its structure to be 6'-N-methylgentamicin C1a and this was confirmed by synthesis from gentamicin C1a. The in vitro antibacterial activity of gentamicin C2b was very similar to that of the gentamicin C complex. Antibiotic XK-62-2, produced by Micromonospora sagamiensis, appears to be identical to gentamicin C1b."}
{"id": "PMID:1092639", "title": "A new prodiginne (prodigiosin-like) pigment from Streptomyces. Antimalarial activity of several prodiginnes.", "content": "Two prodigiosin-like pigments from Streptomyces sp. were shown to be undecylprodiginine (i) and butylcycloheptylprodiginine (v). The antimalarial activity of five prodiginine pigments is given.", "contents": "A new prodiginne (prodigiosin-like) pigment from Streptomyces. Antimalarial activity of several prodiginnes. Two prodigiosin-like pigments from Streptomyces sp. were shown to be undecylprodiginine (i) and butylcycloheptylprodiginine (v). The antimalarial activity of five prodiginine pigments is given."}
{"id": "PMID:1092644", "title": "Insertion of a minor protein into the outer membrane of Escherichia coli during inhibition of lipid synthesis.", "content": "The antibiotic cerulenin, a specific inhibitor of fatty acid synthetase systems, was used to demonstrate that a minor protein component of the outer membrane of Escherichia coli, which serves as the receptor for the phage lambda, can be synthesized and inserted into the outer membrane during inhibition of lipid synthesis.", "contents": "Insertion of a minor protein into the outer membrane of Escherichia coli during inhibition of lipid synthesis. The antibiotic cerulenin, a specific inhibitor of fatty acid synthetase systems, was used to demonstrate that a minor protein component of the outer membrane of Escherichia coli, which serves as the receptor for the phage lambda, can be synthesized and inserted into the outer membrane during inhibition of lipid synthesis."}
{"id": "PMID:1092645", "title": "Thermosensitive mutants of Escherichia coli K-12 altered in the catalytic Subunit and in a Regulatory factor of the glutamy-transfer ribonucleic acid synthetase.", "content": "The glutamyl-transfer ribonucleic acid synthetase (GluRS) of a partial revertants (ts plus or minus) of the thermosensitive (ts) mutant strain JP1449 (LOcus gltx) and of a ts mutant strain EM111-ts1 with a lesion in or near the locus gltx have been studied to find the relation between these two genetic loci known to influence the GluRS activity in vitro and the presence of a catalytic subunit and of a regulatory subunit in the GluRS purified from Escherichia coli K-12. The ts character of strain JP1449-18ts plus or minus is co-transduced with the marker dsdA at the same frequency as is the ts character of strain JP1449. Its purified GluRS is very thermolabile and its Km for glutamate is higher than that of a wild-type GluRS. These results indicate that the locus gltX is in the structural gene for the catalytic subunit of this enzyme. The location of the mutation causing the partial ts reversion in strain JP1449-18ts plus or minus is discussed. The GluRS purified from the ts mutant strain EM111-ts1 has the same stability as the wild-type enzyme, but its Km forglutamate increases with the temperature, suggesting that the locus gltE codes for a regulatory factor, possibly for the polypeptide chain that is co-purified with the catalytic subunit.", "contents": "Thermosensitive mutants of Escherichia coli K-12 altered in the catalytic Subunit and in a Regulatory factor of the glutamy-transfer ribonucleic acid synthetase. The glutamyl-transfer ribonucleic acid synthetase (GluRS) of a partial revertants (ts plus or minus) of the thermosensitive (ts) mutant strain JP1449 (LOcus gltx) and of a ts mutant strain EM111-ts1 with a lesion in or near the locus gltx have been studied to find the relation between these two genetic loci known to influence the GluRS activity in vitro and the presence of a catalytic subunit and of a regulatory subunit in the GluRS purified from Escherichia coli K-12. The ts character of strain JP1449-18ts plus or minus is co-transduced with the marker dsdA at the same frequency as is the ts character of strain JP1449. Its purified GluRS is very thermolabile and its Km for glutamate is higher than that of a wild-type GluRS. These results indicate that the locus gltX is in the structural gene for the catalytic subunit of this enzyme. The location of the mutation causing the partial ts reversion in strain JP1449-18ts plus or minus is discussed. The GluRS purified from the ts mutant strain EM111-ts1 has the same stability as the wild-type enzyme, but its Km forglutamate increases with the temperature, suggesting that the locus gltE codes for a regulatory factor, possibly for the polypeptide chain that is co-purified with the catalytic subunit."}
{"id": "PMID:1092646", "title": "Reduced plasma membrane permeability in a multiple cross-resistant strain of Saccharomyces cerevisiae.", "content": "Single nuclear gene inheritance was shown to be responsible for increased resistance to: eight diverse inhibitors of mitochondrial function (antimycin, carbonylcyanide-m-chlorophenylhydrazone, chloramphenicol, oligomycin, tetracycline, triethyltin bromide, triphenylmethylphosphonium bromide and triton-X-165); and an inhibitor of cytoplasmic protein synthesis (cycloheximide). Continuous monitoring of oxygen uptake during respiratory adaptation showed that anerobic pretreatment of resistant cells sensitized respiratory adaptation to chloramphenicol and antimycin. However, since a depression of mitochondrial function by catabolite repression did not result in sensitization to antimycin, alteration of the mitochondrial membrane does not appear to be responsible for resistance to mitochondrial inhibition. Alteration of cellular binding sites was not responsible for resistance since in vitro mitochondrial protein synthesis was sensitive to chloramphenicol and in vitro mitochondrial respiration was sensitive to oligomycin, carbonylcyanide-m-chlorophenylhydrazone, and antimycin. Autoradiography of an ethylacetate-ethanol extract of [14C]chloramphenicol-treated resistant cells indicated that resistance was not due to enzymatic modification of inhibitors. The maintenance of an antimycin-resistant respiration by protoplasts of resistant cells ruled out the involvement of the cell wall in cellular resistance. The reduced transport of [14C]chloramphenicol by resistant cells (1% of normal cells) indicated that a single nuclear gene mutation can alter the permeability of the plasma membrane to many diverse inhibitors.", "contents": "Reduced plasma membrane permeability in a multiple cross-resistant strain of Saccharomyces cerevisiae. Single nuclear gene inheritance was shown to be responsible for increased resistance to: eight diverse inhibitors of mitochondrial function (antimycin, carbonylcyanide-m-chlorophenylhydrazone, chloramphenicol, oligomycin, tetracycline, triethyltin bromide, triphenylmethylphosphonium bromide and triton-X-165); and an inhibitor of cytoplasmic protein synthesis (cycloheximide). Continuous monitoring of oxygen uptake during respiratory adaptation showed that anerobic pretreatment of resistant cells sensitized respiratory adaptation to chloramphenicol and antimycin. However, since a depression of mitochondrial function by catabolite repression did not result in sensitization to antimycin, alteration of the mitochondrial membrane does not appear to be responsible for resistance to mitochondrial inhibition. Alteration of cellular binding sites was not responsible for resistance since in vitro mitochondrial protein synthesis was sensitive to chloramphenicol and in vitro mitochondrial respiration was sensitive to oligomycin, carbonylcyanide-m-chlorophenylhydrazone, and antimycin. Autoradiography of an ethylacetate-ethanol extract of [14C]chloramphenicol-treated resistant cells indicated that resistance was not due to enzymatic modification of inhibitors. The maintenance of an antimycin-resistant respiration by protoplasts of resistant cells ruled out the involvement of the cell wall in cellular resistance. The reduced transport of [14C]chloramphenicol by resistant cells (1% of normal cells) indicated that a single nuclear gene mutation can alter the permeability of the plasma membrane to many diverse inhibitors."}
{"id": "PMID:1092647", "title": "Existence of two levels of repression in the biosynthesis of methionine in Saccharomyces cerevisiae: effect of lomofungin on enzyme synthesis.", "content": "Derepression of a methionine biosynthetic enzyme (homocysteine synthase) has been studied after repression either by exogenous methionine or by exogenous S-adenosylmethionine (SAM). Lomofungin, which inhibits the synthesis of ribosomal precursor and messenger ribonucleic acid but not of protein in Saccharomyces cerevisiae, has been used in this system. It has been shown that the addition of this antibiotic prevents the derepression of homocysteine synthase after repression by exogenous methionine but not after repression by exogenous SAM. These experiments with lomofungin and the kinetics of repression after addition of methionine or SAM to the growth medium provide evidence that the repression induced by exogenous methionine acts at the transcriptional level whereas the repression induced by exogenous SAM acts at the translational level.", "contents": "Existence of two levels of repression in the biosynthesis of methionine in Saccharomyces cerevisiae: effect of lomofungin on enzyme synthesis. Derepression of a methionine biosynthetic enzyme (homocysteine synthase) has been studied after repression either by exogenous methionine or by exogenous S-adenosylmethionine (SAM). Lomofungin, which inhibits the synthesis of ribosomal precursor and messenger ribonucleic acid but not of protein in Saccharomyces cerevisiae, has been used in this system. It has been shown that the addition of this antibiotic prevents the derepression of homocysteine synthase after repression by exogenous methionine but not after repression by exogenous SAM. These experiments with lomofungin and the kinetics of repression after addition of methionine or SAM to the growth medium provide evidence that the repression induced by exogenous methionine acts at the transcriptional level whereas the repression induced by exogenous SAM acts at the translational level."}
{"id": "PMID:1092648", "title": "Biochemical and regulatory effects of methionine analogues in Saccharomyces cerevisiae.", "content": "The effect of three methionine analogues, ethionine, selenomethionine, and trifluoromethionine, on the biosynthesis of methionine in Saccharomyces cerevisiae has been investigated. We have found the following to be true. (i) A sharp decrease in the endogenous methionine concentration occurs after the addition of any one of these analogues to growing cells. (ii) All of them can be transferred to methionine transfer ribonucleic acid in vitro as well as in vivo with, as a consequence, their incorporation into proteins. In the absence of radioactive trifluoromethionine, this conclusion results from experiments of an indirect nature and must be taken as an indication rather than a direct demonstration. (iii) Ethionine and selenomethionine can be activated as homologues of S-adenosylmethionine, whereas trifluoromethionine cannot. (iv) All of them can act as repressors of the methionine biosynthetic pathway. This has been shown by measuring the de novo rate of synthesis of methionine in a culture grown in the presence of any one of the three analogues.", "contents": "Biochemical and regulatory effects of methionine analogues in Saccharomyces cerevisiae. The effect of three methionine analogues, ethionine, selenomethionine, and trifluoromethionine, on the biosynthesis of methionine in Saccharomyces cerevisiae has been investigated. We have found the following to be true. (i) A sharp decrease in the endogenous methionine concentration occurs after the addition of any one of these analogues to growing cells. (ii) All of them can be transferred to methionine transfer ribonucleic acid in vitro as well as in vivo with, as a consequence, their incorporation into proteins. In the absence of radioactive trifluoromethionine, this conclusion results from experiments of an indirect nature and must be taken as an indication rather than a direct demonstration. (iii) Ethionine and selenomethionine can be activated as homologues of S-adenosylmethionine, whereas trifluoromethionine cannot. (iv) All of them can act as repressors of the methionine biosynthetic pathway. This has been shown by measuring the de novo rate of synthesis of methionine in a culture grown in the presence of any one of the three analogues."}
{"id": "PMID:1092649", "title": "Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains.", "content": "Two of seven sucrose-fermenting Salmonella strains obtained from clinical sources were found capable of conjugal transfer of the sucrose fermentation (Scr+) property to the Escherichia coli K-12 strain WR3026. The genetic elements conferring this Scr+ property, designated scr-53 and scr-94, were then conjugally transmissible from Escherichia coli WR3026 Scr+ exconjugants to other strains of Escherichia coli at frequences of 5 times 10- minus 6 to 5 times 10- minus 3 for the scr-53 element and 10- minus 6 to 10- minus 5 for the scr-94 element. In Escherichia coli hosts, both of these elements were compatible with F-lac and with each of six previously characterized transmissible lac elements. No antibiotic resistance characteristics or colicin production were discovered to be associated with either scr-53 or scr-94. Neither scr element generated a male host response to the female-specific phage phiII, but the scr-53 element rendered its Escherichia coli host sensitive to the male-specific phage R-17. Escherichia coli hosts containing scr-53 were susceptible to lysis by P1vir, and transduction of the scr-53 element was accomplished with this phage. The scr-53 element was isolated from Escherichia coli WR3026, Scr+ transductants, and Escherichia coli WR2036 Scr+ exconjugants as a covalently closed circular deoxyribonucleic acid molecule with a molecular weight (determined by electron microscopy) of approximately 52 times 10-6. Receipt of the scr-94 element rendered Escherichia coli hosts of this element unsusceptible to lysis by P1vir, although adsorption of the phage by an Escherichia coli WR3026 exconjugant containing scr-94 occurred as efficiently as it did on WR3026 itself. Repeated examination of Escherichia coli strains harboring scr-94, as well as of the Salmonella strain which initially contained it, did not reveal the presence of circular deoxyribonucleic acid. The synthesis of the sucrose cleaving enzyme was inducible in Escherichia coli exconjugants containing either scr-53 or scr-94.", "contents": "Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains. Two of seven sucrose-fermenting Salmonella strains obtained from clinical sources were found capable of conjugal transfer of the sucrose fermentation (Scr+) property to the Escherichia coli K-12 strain WR3026. The genetic elements conferring this Scr+ property, designated scr-53 and scr-94, were then conjugally transmissible from Escherichia coli WR3026 Scr+ exconjugants to other strains of Escherichia coli at frequences of 5 times 10- minus 6 to 5 times 10- minus 3 for the scr-53 element and 10- minus 6 to 10- minus 5 for the scr-94 element. In Escherichia coli hosts, both of these elements were compatible with F-lac and with each of six previously characterized transmissible lac elements. No antibiotic resistance characteristics or colicin production were discovered to be associated with either scr-53 or scr-94. Neither scr element generated a male host response to the female-specific phage phiII, but the scr-53 element rendered its Escherichia coli host sensitive to the male-specific phage R-17. Escherichia coli hosts containing scr-53 were susceptible to lysis by P1vir, and transduction of the scr-53 element was accomplished with this phage. The scr-53 element was isolated from Escherichia coli WR3026, Scr+ transductants, and Escherichia coli WR2036 Scr+ exconjugants as a covalently closed circular deoxyribonucleic acid molecule with a molecular weight (determined by electron microscopy) of approximately 52 times 10-6. Receipt of the scr-94 element rendered Escherichia coli hosts of this element unsusceptible to lysis by P1vir, although adsorption of the phage by an Escherichia coli WR3026 exconjugant containing scr-94 occurred as efficiently as it did on WR3026 itself. Repeated examination of Escherichia coli strains harboring scr-94, as well as of the Salmonella strain which initially contained it, did not reveal the presence of circular deoxyribonucleic acid. The synthesis of the sucrose cleaving enzyme was inducible in Escherichia coli exconjugants containing either scr-53 or scr-94."}
{"id": "PMID:1092650", "title": "Events following prophage Mu induction.", "content": "Escherichia coli strains lysogenic for a thermoinducible Mu prophage (Mu cts62) undergo rapid lysis about 50 min after heat induction. Induction of Mu cts62 apparently causes damage to the host sequences in which Mu is inserted. The normal expression of A, BU, and X genes of Mu is needed for this specific deleterious effect on the prophage-containing host sequences. Mu deoxyribonucleic acid can be shown to reintegrate extensively at different sites on the host genome during the lytic cycle after prophage induction or after infection of sensitive cells by clear-plaque mutants of Mu. We estimate that approximately 10 copies of Mu deoxyribonucleic acid are inserted per chromosome during vegetative growth. The episome rescue method for detecting vegetative Mu deoxyribonucleic acid insertion, in which an episome is transferred from the lytically infected cells to F- receipient cells, can be applied to study Mu integration without requiring the host cells to survive. It also provides an easy system to isolate Mu insertions in transmissible episomes and plasmids.", "contents": "Events following prophage Mu induction. Escherichia coli strains lysogenic for a thermoinducible Mu prophage (Mu cts62) undergo rapid lysis about 50 min after heat induction. Induction of Mu cts62 apparently causes damage to the host sequences in which Mu is inserted. The normal expression of A, BU, and X genes of Mu is needed for this specific deleterious effect on the prophage-containing host sequences. Mu deoxyribonucleic acid can be shown to reintegrate extensively at different sites on the host genome during the lytic cycle after prophage induction or after infection of sensitive cells by clear-plaque mutants of Mu. We estimate that approximately 10 copies of Mu deoxyribonucleic acid are inserted per chromosome during vegetative growth. The episome rescue method for detecting vegetative Mu deoxyribonucleic acid insertion, in which an episome is transferred from the lytically infected cells to F- receipient cells, can be applied to study Mu integration without requiring the host cells to survive. It also provides an easy system to isolate Mu insertions in transmissible episomes and plasmids."}
{"id": "PMID:1092651", "title": "Homology between the deoxyribonucleic acid of fertility factor P and Vibrio cholerae chromosomal deoxyribonucleic acid.", "content": "The deoxyribonucleic acid (DNA) of the Vibrio cholerae fertility factor P was isolated by the dye-buoyant density method and hybridized to V. cholerae chromosomal DNA. The DNA of this fertility plasmid had between 35 to 40% homology with the V. cholerae chromosomal DNA. Little or no homology was detected between the P factor DNA and DNA of the Escherichia coli sex factor F.", "contents": "Homology between the deoxyribonucleic acid of fertility factor P and Vibrio cholerae chromosomal deoxyribonucleic acid. The deoxyribonucleic acid (DNA) of the Vibrio cholerae fertility factor P was isolated by the dye-buoyant density method and hybridized to V. cholerae chromosomal DNA. The DNA of this fertility plasmid had between 35 to 40% homology with the V. cholerae chromosomal DNA. Little or no homology was detected between the P factor DNA and DNA of the Escherichia coli sex factor F."}
{"id": "PMID:1092652", "title": "Isolation and characterization of mutator strains of Escherichia coli K-12.", "content": "A selection procedure was devised to select for mutants of Escherichia coli K-12 with enhanced rates of spontaneous frameshift mutation. Three types of mutants were isolated. Two of the mutations apparently represent alleles of previously isolated mutL13 and mutS3. The third type of mutation, represented by two alleles, lies between lysA and thyA, and has been designated mutR. mutR increases the rate of spontaneous frameshift mutation and also the rate of base substitution mutations. The mutator phenotype is recessive. Reversion of a lac amber mutation located on an episome is increased in the presence of the mutator, indicating that mutR can act in trans. No change in sensitivity to ultraviolet irradiation or mitomycin C could be found when mutR34 was compared to the isogenic mutR+ strain. The mutator's activity was little affected by the type of medium in which the strain was grown. Deoxyribonucleoside triphosphate pools were normal in mutR34. Intergenic recombination frequencies were the same in mutR and mutR and mutR+ strains, but a two- to threefold increase in intragenic recombination was observed in Hfr times Fminus crosses when the recipeint was mutR34 as compared with mutR+. This increase appeared independent of the distance between the two markers within the gene in which the crossover took place.", "contents": "Isolation and characterization of mutator strains of Escherichia coli K-12. A selection procedure was devised to select for mutants of Escherichia coli K-12 with enhanced rates of spontaneous frameshift mutation. Three types of mutants were isolated. Two of the mutations apparently represent alleles of previously isolated mutL13 and mutS3. The third type of mutation, represented by two alleles, lies between lysA and thyA, and has been designated mutR. mutR increases the rate of spontaneous frameshift mutation and also the rate of base substitution mutations. The mutator phenotype is recessive. Reversion of a lac amber mutation located on an episome is increased in the presence of the mutator, indicating that mutR can act in trans. No change in sensitivity to ultraviolet irradiation or mitomycin C could be found when mutR34 was compared to the isogenic mutR+ strain. The mutator's activity was little affected by the type of medium in which the strain was grown. Deoxyribonucleoside triphosphate pools were normal in mutR34. Intergenic recombination frequencies were the same in mutR and mutR and mutR+ strains, but a two- to threefold increase in intragenic recombination was observed in Hfr times Fminus crosses when the recipeint was mutR34 as compared with mutR+. This increase appeared independent of the distance between the two markers within the gene in which the crossover took place."}
{"id": "PMID:1092653", "title": "Multiple gene loci for a single species of glycine transfer ribonucleic acid.", "content": "The study of suppressors of tryptophan synthase A protein missense mutations in Escherichia coli has led to the establishment of two nonadjacent genetic loci (gly V and gly W) specifying identical nucleotide sequences for a single isoaccepting species of glycine transfer ribonucleic acid (tRNA GLY 3 GGU/C). In one case, suppression of the missense mutation trpA78 was due to a mutation in a structural gene (gly W) for tRNA Gly 3 GGU/C. This mutation resulted in a base change in the anticodon and modification of an A residue adjacent to the 3' side of the anticodon, leading to the production of a tRNA Gly 3 UGU/C species. The resulting glyW51 (SU UGU/C) allele was mapped by interrupted mating and was located at approximately 37 min on the Escherichia coli genetic map. Other suppressor mutations affecting the primary sequence of tRNA Gly GGU/C and giving rise to the Ins and SU+A58 phenotypes were positioned at 86 min (glyV). Several independently arising missense suppressor mutations resulting in the SU+A78 phenotypes were isolated and mapped at these two genetic loci (glyV and glyW). The ratio of appearance of suppressor mutations at glyV and glyW suggests that there are three of four tRNAGly3 GGU/C structural gene copies at the glyV locus to one copy at the glyW locus. Structural genes for tRNA ly isoacceptors are now known at four distinct locations on the Escherichia coli chromosome: glyT (77 MIN), TRNA Gly 2 GGA/G; gly U (55 min), tRNAGly-1 minus; and gly V (86 MIN) AND GLYW (37 min), tRNAGly 3 GGU/C.", "contents": "Multiple gene loci for a single species of glycine transfer ribonucleic acid. The study of suppressors of tryptophan synthase A protein missense mutations in Escherichia coli has led to the establishment of two nonadjacent genetic loci (gly V and gly W) specifying identical nucleotide sequences for a single isoaccepting species of glycine transfer ribonucleic acid (tRNA GLY 3 GGU/C). In one case, suppression of the missense mutation trpA78 was due to a mutation in a structural gene (gly W) for tRNA Gly 3 GGU/C. This mutation resulted in a base change in the anticodon and modification of an A residue adjacent to the 3' side of the anticodon, leading to the production of a tRNA Gly 3 UGU/C species. The resulting glyW51 (SU UGU/C) allele was mapped by interrupted mating and was located at approximately 37 min on the Escherichia coli genetic map. Other suppressor mutations affecting the primary sequence of tRNA Gly GGU/C and giving rise to the Ins and SU+A58 phenotypes were positioned at 86 min (glyV). Several independently arising missense suppressor mutations resulting in the SU+A78 phenotypes were isolated and mapped at these two genetic loci (glyV and glyW). The ratio of appearance of suppressor mutations at glyV and glyW suggests that there are three of four tRNAGly3 GGU/C structural gene copies at the glyV locus to one copy at the glyW locus. Structural genes for tRNA ly isoacceptors are now known at four distinct locations on the Escherichia coli chromosome: glyT (77 MIN), TRNA Gly 2 GGA/G; gly U (55 min), tRNAGly-1 minus; and gly V (86 MIN) AND GLYW (37 min), tRNAGly 3 GGU/C."}
{"id": "PMID:1092654", "title": "Five control systems preventing transfer of Escherichia coli K-12 sex factor F.", "content": "The transfer inhibition systems of 28 Fin+ plasmids have been characterized, using Flac mutants insensitive to inhibition by R100 or R62. All F-like plasmids (except R455) and one N group plasmid determined systems analogous to that of R100; this is designated the FinOP system. None of these plasmids could supply a FinP component of the transfer inhibitor able to replace that of F itself. In addition to the FinOP and R62 transfer inhibition systems described previously, new systems were encoded by the F-like plasmid R455, the I-like plasmid JR66a, and the group X plasmid R485. Besides inhibiting F transfer, JR66a also inhibited F pilus formation and surface exclusion, whereas R485 inhibited only pilus formation and R455 inhibited neither. All three R factors inhibited transfer of J-independent Flac elements, indicating that they act directly on one or more genes (or products) of the transfer operon, rather than directly via traJ. The tral products and transfer origin sequences of two Fin+ F-like plasmids, ColB2 and R124, appear to have similar specificities to those of F itself.", "contents": "Five control systems preventing transfer of Escherichia coli K-12 sex factor F. The transfer inhibition systems of 28 Fin+ plasmids have been characterized, using Flac mutants insensitive to inhibition by R100 or R62. All F-like plasmids (except R455) and one N group plasmid determined systems analogous to that of R100; this is designated the FinOP system. None of these plasmids could supply a FinP component of the transfer inhibitor able to replace that of F itself. In addition to the FinOP and R62 transfer inhibition systems described previously, new systems were encoded by the F-like plasmid R455, the I-like plasmid JR66a, and the group X plasmid R485. Besides inhibiting F transfer, JR66a also inhibited F pilus formation and surface exclusion, whereas R485 inhibited only pilus formation and R455 inhibited neither. All three R factors inhibited transfer of J-independent Flac elements, indicating that they act directly on one or more genes (or products) of the transfer operon, rather than directly via traJ. The tral products and transfer origin sequences of two Fin+ F-like plasmids, ColB2 and R124, appear to have similar specificities to those of F itself."}
{"id": "PMID:1092655", "title": "Formation of chromatographically unique species of transfer ribonucleic acid during amino acid starvation of relaxed-control Escherichia coli.", "content": "Examination of the transfer ribonucleic acid (tRNA) produced by starving, relaxed-control (rel minus) strains of Escherichia coli for required amino acids revealed the occurrence of a number of chromatographically unique subspecies. Leucine starvation results in the formation of new isoacceptor species of leucine-, histidine-, arginine-, valine-, and phenylalanine-specific tRNA and quantitative changes in the column profiles of serine, glycine, and isoleucine tRNA. Evidence that the unique tRNA species are synthesized de novo during amino acid starvation comes from the findings that the major unique leucine isoacceptor species is not formed in stringent control cells or in rel minus cells starved for uracil or treated with rifampin. Furthermore, heat treatment of the unique leucine tRNA does not alter its chromatographic behavior, indicating that the species is not an aggregate or nuclease-damaged form of a normal isoacceptor tRNA. The methyl acceptor activities of tRNA from leucine-starved and nonstarved rel+ or rel minus cells were found to be essentially the same. This result and the finding that the chromatographic behavior of the unique leucine-specific tRNA was not altered after treatment with tRNA methylase suggests that gross methyl deficiency is probably not the biochemical basis for the occurrence of the unique species.", "contents": "Formation of chromatographically unique species of transfer ribonucleic acid during amino acid starvation of relaxed-control Escherichia coli. Examination of the transfer ribonucleic acid (tRNA) produced by starving, relaxed-control (rel minus) strains of Escherichia coli for required amino acids revealed the occurrence of a number of chromatographically unique subspecies. Leucine starvation results in the formation of new isoacceptor species of leucine-, histidine-, arginine-, valine-, and phenylalanine-specific tRNA and quantitative changes in the column profiles of serine, glycine, and isoleucine tRNA. Evidence that the unique tRNA species are synthesized de novo during amino acid starvation comes from the findings that the major unique leucine isoacceptor species is not formed in stringent control cells or in rel minus cells starved for uracil or treated with rifampin. Furthermore, heat treatment of the unique leucine tRNA does not alter its chromatographic behavior, indicating that the species is not an aggregate or nuclease-damaged form of a normal isoacceptor tRNA. The methyl acceptor activities of tRNA from leucine-starved and nonstarved rel+ or rel minus cells were found to be essentially the same. This result and the finding that the chromatographic behavior of the unique leucine-specific tRNA was not altered after treatment with tRNA methylase suggests that gross methyl deficiency is probably not the biochemical basis for the occurrence of the unique species."}
{"id": "PMID:1092656", "title": "Hycanthone and its congeners as bacterial mutagens.", "content": "Hycanthone methanesulfonate (HCT) was shown to induce \"forward\" and \"reverse\" mutations in Salmonella typhimurium and Escherichia coli. Mutational effects of HCT on S. tyhhimurium TA1532 were concentration and time dependent. A comparison of mutagenic potency for TA1532 was made between HCT and the frame-shift mutagens quinacrine and ICR-191. An investigation of structure-activity relationships revealed the substituent in the 4-position of ring A to be critical for mutagenicity. Activity was found when this group was a hydroxymethyl (i.e., HCT) or an aldehyde (Win 25,315), but the analogues having a carboxyl group (Win 25,850) or methyl group (lucanthone) in this positionwere inactive. Removal of a single ethyl group from the side chain did not affect mutagenic activity inasmuch as the potency of desethyl HCT (Win 27,262) equaled that of HCT on a molar basis. A marginal activity was found with a sample of HCT sulfoxide (win 27,266), but this sample was found to contain traces of HCT. The HCT analogue with a terminal N-oxide in the side chain (Win 29,329) was inactive at the concentration tested.", "contents": "Hycanthone and its congeners as bacterial mutagens. Hycanthone methanesulfonate (HCT) was shown to induce \"forward\" and \"reverse\" mutations in Salmonella typhimurium and Escherichia coli. Mutational effects of HCT on S. tyhhimurium TA1532 were concentration and time dependent. A comparison of mutagenic potency for TA1532 was made between HCT and the frame-shift mutagens quinacrine and ICR-191. An investigation of structure-activity relationships revealed the substituent in the 4-position of ring A to be critical for mutagenicity. Activity was found when this group was a hydroxymethyl (i.e., HCT) or an aldehyde (Win 25,315), but the analogues having a carboxyl group (Win 25,850) or methyl group (lucanthone) in this positionwere inactive. Removal of a single ethyl group from the side chain did not affect mutagenic activity inasmuch as the potency of desethyl HCT (Win 27,262) equaled that of HCT on a molar basis. A marginal activity was found with a sample of HCT sulfoxide (win 27,266), but this sample was found to contain traces of HCT. The HCT analogue with a terminal N-oxide in the side chain (Win 29,329) was inactive at the concentration tested."}
{"id": "PMID:1092657", "title": "Rapid cessation of phospholipid synthesis in fructose-1,6-diphosphate aldolase mutants of Escherichia coli.", "content": "Escherichia coli GH352, which was originally described as a temperature-sensitive strain containing a thermolabile acyl coenzyme A:monoacylglycerol 3-phosphate acyltransferase, does not now contain a thermolabile form of this enzyme. It has a defect in fructose-1,6-diphosphate aldolase and at least one additional temperature-sensitive lesion. Both strains GH352 and NP315, a temperature-sensitive aldolase mutant, show rapid cessation of 32-P1 incorporation into nucleic acids and phospholipids at 42 C. These characteristics of strain GH352 are therefore no longer attributed to thermolabile phospholipid synthesis, but can be attributed to the fructose-1,6-diphophate aldolase lesion.", "contents": "Rapid cessation of phospholipid synthesis in fructose-1,6-diphosphate aldolase mutants of Escherichia coli. Escherichia coli GH352, which was originally described as a temperature-sensitive strain containing a thermolabile acyl coenzyme A:monoacylglycerol 3-phosphate acyltransferase, does not now contain a thermolabile form of this enzyme. It has a defect in fructose-1,6-diphosphate aldolase and at least one additional temperature-sensitive lesion. Both strains GH352 and NP315, a temperature-sensitive aldolase mutant, show rapid cessation of 32-P1 incorporation into nucleic acids and phospholipids at 42 C. These characteristics of strain GH352 are therefore no longer attributed to thermolabile phospholipid synthesis, but can be attributed to the fructose-1,6-diphophate aldolase lesion."}
{"id": "PMID:1092658", "title": "Mapping of sul, the suppressor of lon in Escherichia coli.", "content": "The suppressor sul, which is allele specific for the ultraviolet sensitivity gene lon, has been mapped by conjugation and transductional crosses in Escherichia coli K-12 and B/r. Previously, sul was reported to lie in the azi region of the Escherichia coli chromosome. Evidence is presented which positions sul close to and clockwise of fabA on the Escherichia coli map. Cotransductional frequencies of 31.3% were obtained between sul and pyrD, and frequencies of 82% were obtained between sul and fabA. Also, the mucoid phenotype of K-12 lon strains grown on minimal glucose agar plates at 37 C was not significantly effected in sul derivatives. No differences between the sul of Escherichia coli B/r and that of K-12 derivatives with regard to map location or effect on mucoid production were observed.", "contents": "Mapping of sul, the suppressor of lon in Escherichia coli. The suppressor sul, which is allele specific for the ultraviolet sensitivity gene lon, has been mapped by conjugation and transductional crosses in Escherichia coli K-12 and B/r. Previously, sul was reported to lie in the azi region of the Escherichia coli chromosome. Evidence is presented which positions sul close to and clockwise of fabA on the Escherichia coli map. Cotransductional frequencies of 31.3% were obtained between sul and pyrD, and frequencies of 82% were obtained between sul and fabA. Also, the mucoid phenotype of K-12 lon strains grown on minimal glucose agar plates at 37 C was not significantly effected in sul derivatives. No differences between the sul of Escherichia coli B/r and that of K-12 derivatives with regard to map location or effect on mucoid production were observed."}
{"id": "PMID:1092659", "title": "Simple downshift and resulting lack of correlation between ppGpp pool size and ribonucleic acid accumulation.", "content": "The growth rate of Escherichia coli can be limited by the availability of carbon and energy. To impose such a limitation, alpha-methylglucoside (alpha MG), a non-metabolizable analogue, can be used to decrease uptake of glucose by competition for the transport of this sugar. Varying the ratio of glucose to alphaMG allowed shifts in growth rate without simultaneous qualitative changes in the growth medium and permitted examination of the immediate changes accompanying such shifts. Stringent (rel+) as well as relaxed (rel minus) strains were able to rapidly curtail their accumulation of ribonculeic acid (RNA) after a downshift imposed by decreasing glucose transport into the cell. Guanosine 5'-diphosphate 3'-diphosphate (ppGpp) accumulated in both rel+ and rel minus strains after a degrease in growth rate. However, the accumulation of ppGpp in relaxed derivatives was very slow, and there was no direct or obligatory correlation between the level of ppGpp and the rate of RNA accumulation. This latter conclusion is supported by measurements of ppGpp levels and rates of RNA accumulation after restoration of maximal growth rates by addition of excess glucose.", "contents": "Simple downshift and resulting lack of correlation between ppGpp pool size and ribonucleic acid accumulation. The growth rate of Escherichia coli can be limited by the availability of carbon and energy. To impose such a limitation, alpha-methylglucoside (alpha MG), a non-metabolizable analogue, can be used to decrease uptake of glucose by competition for the transport of this sugar. Varying the ratio of glucose to alphaMG allowed shifts in growth rate without simultaneous qualitative changes in the growth medium and permitted examination of the immediate changes accompanying such shifts. Stringent (rel+) as well as relaxed (rel minus) strains were able to rapidly curtail their accumulation of ribonculeic acid (RNA) after a downshift imposed by decreasing glucose transport into the cell. Guanosine 5'-diphosphate 3'-diphosphate (ppGpp) accumulated in both rel+ and rel minus strains after a degrease in growth rate. However, the accumulation of ppGpp in relaxed derivatives was very slow, and there was no direct or obligatory correlation between the level of ppGpp and the rate of RNA accumulation. This latter conclusion is supported by measurements of ppGpp levels and rates of RNA accumulation after restoration of maximal growth rates by addition of excess glucose."}
{"id": "PMID:1092660", "title": "Effect of ribonuclease on the association of deoxyribonucleic acid with the membrane in Escherichia coli.", "content": "The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from the activation, by RNase, of endonuclease I and subsequent limited activity of this deoxyribonuclease. In support of this explanation, it is demonstrated (i) that the extent of the RNase-induced loss of DNA from membrane is directly correlated with the endogenous level of endonuclease I, and (ii) that endonucleolytic activity occurs when gently lysed cell preparations are incubated in the presence of RNase.", "contents": "Effect of ribonuclease on the association of deoxyribonucleic acid with the membrane in Escherichia coli. The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from the activation, by RNase, of endonuclease I and subsequent limited activity of this deoxyribonuclease. In support of this explanation, it is demonstrated (i) that the extent of the RNase-induced loss of DNA from membrane is directly correlated with the endogenous level of endonuclease I, and (ii) that endonucleolytic activity occurs when gently lysed cell preparations are incubated in the presence of RNase."}
{"id": "PMID:1092661", "title": "Potassium translocation in yeast mitochondria and its relationship to ergostrol biosynthesis.", "content": "The energy-dependent transport and accumulation of K+ in respiring mitochondria has been found to inhibit the S-adenosylmethionine: delta-24-sterol methyltransferase enzyme of Saccharomyces cerevisiae. Potassium cation translocation is discussed as a possible regulatory mechanism over the biosynthesis of ergosterol.", "contents": "Potassium translocation in yeast mitochondria and its relationship to ergostrol biosynthesis. The energy-dependent transport and accumulation of K+ in respiring mitochondria has been found to inhibit the S-adenosylmethionine: delta-24-sterol methyltransferase enzyme of Saccharomyces cerevisiae. Potassium cation translocation is discussed as a possible regulatory mechanism over the biosynthesis of ergosterol."}
{"id": "PMID:1092662", "title": "Reinitiation of chromosome replication in the presence of chloramphenicol under an integratively suppressed state by R6K.", "content": "The autonomous replication of an R plasmid, R6K (amp, str) was shown not to be affected by chloramphenicol. It provoked integrative suppression and gave rise to Hfr strains when integrated into the chromosome of a strain of Escherichia coli K-12 with a temperature-sensitive mutation in the gene, dnaA. An Hfr strain designated as Hfr(R6K) no. 1 was thus obtained and characterized. It was not completely stable as shown by a plating efficiency of 0.6 at 42 C relative to that at 30 C. The density labeling and the ultracentrifugation analysis suggested that the deoxyribonucleic acid replication in this Hfr strain did not stop immediately after completion of the round already started before temperature shift-up and the addition of chloramphenicol. These observations are discussed in relation to a possibility that the chromosome replication of this Hfr strain is under the control of the integrated plasmid at a nonpermissive temperature.", "contents": "Reinitiation of chromosome replication in the presence of chloramphenicol under an integratively suppressed state by R6K. The autonomous replication of an R plasmid, R6K (amp, str) was shown not to be affected by chloramphenicol. It provoked integrative suppression and gave rise to Hfr strains when integrated into the chromosome of a strain of Escherichia coli K-12 with a temperature-sensitive mutation in the gene, dnaA. An Hfr strain designated as Hfr(R6K) no. 1 was thus obtained and characterized. It was not completely stable as shown by a plating efficiency of 0.6 at 42 C relative to that at 30 C. The density labeling and the ultracentrifugation analysis suggested that the deoxyribonucleic acid replication in this Hfr strain did not stop immediately after completion of the round already started before temperature shift-up and the addition of chloramphenicol. These observations are discussed in relation to a possibility that the chromosome replication of this Hfr strain is under the control of the integrated plasmid at a nonpermissive temperature."}
{"id": "PMID:1092663", "title": "Lipophilic chelator inhibition of electron transport in Escherichia coli.", "content": "The lipophilic chelator bathophenanthroline inhibits electron transport in membranes from Escherichia coli. The less lipophilic 1,10-phenanthroline, bathophenanthroline sulfonate, and alpha,alpha-dipyridyl have little effect. Reduced nicotinamide adenine dinucleotide oxidase is more sensitive to bathophenanthroline inhibition than lactate oxidase activity. Evidence for two sites of inhibition comes from the fact that both reduced nicotinamide adenine dinucleotide menadione reductase and duroquinol oxidase activities are inhibited. Addition of uncouplers of phosphorylation before bathophenanthroline protects against inhibition.", "contents": "Lipophilic chelator inhibition of electron transport in Escherichia coli. The lipophilic chelator bathophenanthroline inhibits electron transport in membranes from Escherichia coli. The less lipophilic 1,10-phenanthroline, bathophenanthroline sulfonate, and alpha,alpha-dipyridyl have little effect. Reduced nicotinamide adenine dinucleotide oxidase is more sensitive to bathophenanthroline inhibition than lactate oxidase activity. Evidence for two sites of inhibition comes from the fact that both reduced nicotinamide adenine dinucleotide menadione reductase and duroquinol oxidase activities are inhibited. Addition of uncouplers of phosphorylation before bathophenanthroline protects against inhibition."}
{"id": "PMID:1092664", "title": "Morphological change in the early stages of the mating process of Rhodosporidium toruloides.", "content": "The events which occur in the early stages of the mating process of the yeast Rhodosporidium toruloides between strains M-919 (mating type A) and M-1057 (mating type a) were investigated. In preliminary experiments we determined the frequency of mating by two newly designed methods: the liquid culture method and the membrane-filter microculture method. The mating frequencies of strains M-919 and M-1057 were 89% in the liquid culture method and 62% in the membrane-filter microculture method. The early stages in the mating process included the following events: (i) M-919 cells produce constitutively the extracellular inducing substance (A factor), (ii) M-1057 cells receive A factor, and in response to it they form mating tubes and secrete another inducing substance (a factor), (iii) M-919 cells receive a factor, and in response to it they form mating tubes, (iv) mating tubes elongate to the cells or the tubes of mating partner, (v) tips of the growing tubes recognize the opposite mating type cells or their tubes, followed by cell-to-cell fusion.", "contents": "Morphological change in the early stages of the mating process of Rhodosporidium toruloides. The events which occur in the early stages of the mating process of the yeast Rhodosporidium toruloides between strains M-919 (mating type A) and M-1057 (mating type a) were investigated. In preliminary experiments we determined the frequency of mating by two newly designed methods: the liquid culture method and the membrane-filter microculture method. The mating frequencies of strains M-919 and M-1057 were 89% in the liquid culture method and 62% in the membrane-filter microculture method. The early stages in the mating process included the following events: (i) M-919 cells produce constitutively the extracellular inducing substance (A factor), (ii) M-1057 cells receive A factor, and in response to it they form mating tubes and secrete another inducing substance (a factor), (iii) M-919 cells receive a factor, and in response to it they form mating tubes, (iv) mating tubes elongate to the cells or the tubes of mating partner, (v) tips of the growing tubes recognize the opposite mating type cells or their tubes, followed by cell-to-cell fusion."}
{"id": "PMID:1092665", "title": "Endogenous messenger ribonucleic acid-directed polypeptide chain elongation in a cell-free system from the yeast Saccharomyces cerevisiae.", "content": "An in vitro protein-synthesizing system from the yeast Saccharomyces cerevisiae has been made by a modification of the procedure for preparation of the Krebs ascites system. The protein synthetic activity is directed by endogenous messenger. Amino acid incorporation occurs over a broad range of magnesium and potassium concentration, being maximal at 6 and 85 mM, respcetively. The activity of this in vitro system is due to the elongation of polypeptides whose synthesis was initiated in vivo. The cell extract does not initiate synthesis with endogenous messenger ribonucleic acid (RNA), since 1 muM pactamycin, which blocks initiation on prokaryotic or eukaryotic ribosomes invitro, fails to decrease amino acid incorporation. Ten micromolar cycloheximide, however, inhibits incorporation by 87%. Moreover, this system is not stimulated by rabbit reticulocyte polysomal RNA, which directs the synthesis of hemoglobin in extracts of Krebs ascites cells. The translation of this messenger is not masked by high endogenous incorporation, because autoradiography of sodium dodecyl sulfate-polyacrylamide gels containing [35-S]methionine-labeled products shows that no hemoglobin is made. Preincubation of this system, which reduces the high endogenous incorporation by 80%, does not increase its capacity to be stimulated by either rabbit reticulocyte RNA or yeast polyriboadenylic acid-containing RNA. Polyuridylic acid, however, does stimulate polyphenylalanine incorporation. The failure of the yeast lysate to be stimulated by or to translate added natural messenger RNA, its insensitivity to low levels of pactamycin but inhibition by cycloheximide, and its relatively high magnesium optimum (the same as that for polyuridylic acid) suggest that it elongates but does not initiate polypeptide chains.", "contents": "Endogenous messenger ribonucleic acid-directed polypeptide chain elongation in a cell-free system from the yeast Saccharomyces cerevisiae. An in vitro protein-synthesizing system from the yeast Saccharomyces cerevisiae has been made by a modification of the procedure for preparation of the Krebs ascites system. The protein synthetic activity is directed by endogenous messenger. Amino acid incorporation occurs over a broad range of magnesium and potassium concentration, being maximal at 6 and 85 mM, respcetively. The activity of this in vitro system is due to the elongation of polypeptides whose synthesis was initiated in vivo. The cell extract does not initiate synthesis with endogenous messenger ribonucleic acid (RNA), since 1 muM pactamycin, which blocks initiation on prokaryotic or eukaryotic ribosomes invitro, fails to decrease amino acid incorporation. Ten micromolar cycloheximide, however, inhibits incorporation by 87%. Moreover, this system is not stimulated by rabbit reticulocyte polysomal RNA, which directs the synthesis of hemoglobin in extracts of Krebs ascites cells. The translation of this messenger is not masked by high endogenous incorporation, because autoradiography of sodium dodecyl sulfate-polyacrylamide gels containing [35-S]methionine-labeled products shows that no hemoglobin is made. Preincubation of this system, which reduces the high endogenous incorporation by 80%, does not increase its capacity to be stimulated by either rabbit reticulocyte RNA or yeast polyriboadenylic acid-containing RNA. Polyuridylic acid, however, does stimulate polyphenylalanine incorporation. The failure of the yeast lysate to be stimulated by or to translate added natural messenger RNA, its insensitivity to low levels of pactamycin but inhibition by cycloheximide, and its relatively high magnesium optimum (the same as that for polyuridylic acid) suggest that it elongates but does not initiate polypeptide chains."}
{"id": "PMID:1092666", "title": "Isolation and characterization of lambda transducing bacteriophages for argF, argI and adjacent genes.", "content": "Two genes for ornithinetranscarbamylase exist in strain Escherichia coli K-12, argI, at 85 min, and argF, at 7 min. In an attempt to compare the deoxyribonucleic acid material of these two genes, the lambda transducing phages carrying a portion of the argI region, lambda dvalS argI, lambda pvalS, and lambda dvalS pyrB, and of the argF region, lambda dargF, have been isolated. Their structure, including that of phi 80dargF previously isolated, was studied by the method of heteroduplex mapping. In this paper, the results of this mapping are reported.", "contents": "Isolation and characterization of lambda transducing bacteriophages for argF, argI and adjacent genes. Two genes for ornithinetranscarbamylase exist in strain Escherichia coli K-12, argI, at 85 min, and argF, at 7 min. In an attempt to compare the deoxyribonucleic acid material of these two genes, the lambda transducing phages carrying a portion of the argI region, lambda dvalS argI, lambda pvalS, and lambda dvalS pyrB, and of the argF region, lambda dargF, have been isolated. Their structure, including that of phi 80dargF previously isolated, was studied by the method of heteroduplex mapping. In this paper, the results of this mapping are reported."}
{"id": "PMID:1092667", "title": "Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes.", "content": "The structure of F13, a plasmid containing lac, purE, and proC, has been determined by heteroduplex analysis. As expected for an F-prime formed by a type II excision event, it contains all the sequences of F plus a large segment of Escherichia coli chromosomal deoxyribonucleic acid. There is a sequence of F with coordinates 16.3-17.6F which has been shown in other studies to be the insertion sequence IS2. This IS2 occurs twice on F13, once at each of the two junctions of F deoxyribonucleic acid with chromosomal deoxyribonucleic acid. The sequence alpha beta which occurs twice on F with coordinates 93.2-94.5/OF and 13.7-15.0F occurs an additional three times, twice in an inverted order relative to the alpha beta sequences of F, on the chromosomal sequences of F13. The structures of the plasmids F13-4 and F210 have been determined. The common sequences of F13 with F152-1 (a derivative of F152, the classical F2gal) and with F13-4 and F210 have been mapped. These results partially map lac, proC, tsx, and purE on F13. On the basis of all of these results, it is proposed that Hfr 13 (the parent of F13) was formed by recirpocal recombination between IS2 on F and an IS2 resident at a point between lac and proC on the chromosome of the F+ parent of Hfr 13. It is proposed that this IS2 and the several alpha beta sequences on the chromosomal part of F13 are hot spots for recombination with F, i.e., for Hfr formation. The point of origin and direction of transfer of many Hfr's can be explained by this hypothesis. In particular, the sequence relations of F42-1 (Flac) and of F152-1 (F 2gal) with F13 are completely consistent with this model.", "contents": "Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes. The structure of F13, a plasmid containing lac, purE, and proC, has been determined by heteroduplex analysis. As expected for an F-prime formed by a type II excision event, it contains all the sequences of F plus a large segment of Escherichia coli chromosomal deoxyribonucleic acid. There is a sequence of F with coordinates 16.3-17.6F which has been shown in other studies to be the insertion sequence IS2. This IS2 occurs twice on F13, once at each of the two junctions of F deoxyribonucleic acid with chromosomal deoxyribonucleic acid. The sequence alpha beta which occurs twice on F with coordinates 93.2-94.5/OF and 13.7-15.0F occurs an additional three times, twice in an inverted order relative to the alpha beta sequences of F, on the chromosomal sequences of F13. The structures of the plasmids F13-4 and F210 have been determined. The common sequences of F13 with F152-1 (a derivative of F152, the classical F2gal) and with F13-4 and F210 have been mapped. These results partially map lac, proC, tsx, and purE on F13. On the basis of all of these results, it is proposed that Hfr 13 (the parent of F13) was formed by recirpocal recombination between IS2 on F and an IS2 resident at a point between lac and proC on the chromosome of the F+ parent of Hfr 13. It is proposed that this IS2 and the several alpha beta sequences on the chromosomal part of F13 are hot spots for recombination with F, i.e., for Hfr formation. The point of origin and direction of transfer of many Hfr's can be explained by this hypothesis. In particular, the sequence relations of F42-1 (Flac) and of F152-1 (F 2gal) with F13 are completely consistent with this model."}
{"id": "PMID:1092668", "title": "Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids.", "content": "Heteroduplex experiments between the plasmid R6 and one strand of the deoxyribonucleic acid (DNA) of a lambda phage carrying the insertion sequence IS1 show that IS1 occurs on R6 at the two previously mapped junctions of resistance transfer factor (RTF) DNA with R-determinant DNA. From previous heteroduplex experiments, it then follows that IS1 occurs at the same junctions in R6-5, R100-1, and R1 plasmids. Heteroduplex experiments with the DNA from a lambda phage carrying the insertion sequence IS2 show that one copy of IS2 occurs in R6, R6-5, and R100-1 (but not R1) at a point within the RTF with coordinates 67.5 TO 68.9 kilobase units (kb). In an accompanying paper, Ptashne and Cohen (1975) show that the insertion sequence IS3 occurs on R6 and R6-5. R100-25, a traC mutant, differs from its parent R100-1 only in that it contains an additional copy of IS1 inserted within the tra gene region of 82.1 kb. R100-31, atraX, TC-s mutant of R100-1, is deleted in R100-1 sequences starting at one of the IS3 termini (46.9 kb) and extending with RTF to 61.0 kb. Heteroduplex studies of F plasmids with the DNA of a lambda phage bearing insertion sequence IS2 show that the sequence of F with coordinates 16.3-17.6F is IS2. The occurrence of IS1 at the two junctions of R-determinant DNA and RTF DNA in R plasmids provides a structural basis to explain the mechanism of the previously observed formation of molecules containing one RTF unit and several tandem copies of the R-determinant unit, when R plasmids in Proteus mirabilis are grown in the presence of antibiotics, and the segregation of an R plasmid into an RTF unit and an R-determinant unit. In general, correlation of our results with previous studies shows that insertion sequences play a role in a variety of F- and R-related intra- and intermolecular recombination phenomena.", "contents": "Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. Heteroduplex experiments between the plasmid R6 and one strand of the deoxyribonucleic acid (DNA) of a lambda phage carrying the insertion sequence IS1 show that IS1 occurs on R6 at the two previously mapped junctions of resistance transfer factor (RTF) DNA with R-determinant DNA. From previous heteroduplex experiments, it then follows that IS1 occurs at the same junctions in R6-5, R100-1, and R1 plasmids. Heteroduplex experiments with the DNA from a lambda phage carrying the insertion sequence IS2 show that one copy of IS2 occurs in R6, R6-5, and R100-1 (but not R1) at a point within the RTF with coordinates 67.5 TO 68.9 kilobase units (kb). In an accompanying paper, Ptashne and Cohen (1975) show that the insertion sequence IS3 occurs on R6 and R6-5. R100-25, a traC mutant, differs from its parent R100-1 only in that it contains an additional copy of IS1 inserted within the tra gene region of 82.1 kb. R100-31, atraX, TC-s mutant of R100-1, is deleted in R100-1 sequences starting at one of the IS3 termini (46.9 kb) and extending with RTF to 61.0 kb. Heteroduplex studies of F plasmids with the DNA of a lambda phage bearing insertion sequence IS2 show that the sequence of F with coordinates 16.3-17.6F is IS2. The occurrence of IS1 at the two junctions of R-determinant DNA and RTF DNA in R plasmids provides a structural basis to explain the mechanism of the previously observed formation of molecules containing one RTF unit and several tandem copies of the R-determinant unit, when R plasmids in Proteus mirabilis are grown in the presence of antibiotics, and the segregation of an R plasmid into an RTF unit and an R-determinant unit. In general, correlation of our results with previous studies shows that insertion sequences play a role in a variety of F- and R-related intra- and intermolecular recombination phenomena."}
{"id": "PMID:1092669", "title": "Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications.", "content": "Insertion sequence (IS) regions have been identified previously as a cause of strongly polar mutations in Escherichia coli and several bacteriophages. The present experiments indicate that genetically characterized IS regions occur on bacterial plasmid deoxyribonucleic acid (DNA) as both direct and inverted DNA sequence duplications. The DNA insertion which has been shown previously (Sharp et al., 1973) to control expression of tetracycline resistance in the R6-5 plasmid, and which occurs as directly and inversely repeated DNA sequences adjacent to the region believed to contain the tetracycline resistance gene, has been identified as IS3. A second genetically characterized insertion sequence (IS1) has been identified as a direct DNA duplication occurring at both junctions of the resistance transfer factor and R-determinant components of R6-5 and related plasmids. A model is presented for the reversible dissociation of resistance transfer factor and R-determinant components of co-integrate R plasmids at the sites of DNA sequence homology provided by the repeated IS regions.", "contents": "Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. Insertion sequence (IS) regions have been identified previously as a cause of strongly polar mutations in Escherichia coli and several bacteriophages. The present experiments indicate that genetically characterized IS regions occur on bacterial plasmid deoxyribonucleic acid (DNA) as both direct and inverted DNA sequence duplications. The DNA insertion which has been shown previously (Sharp et al., 1973) to control expression of tetracycline resistance in the R6-5 plasmid, and which occurs as directly and inversely repeated DNA sequences adjacent to the region believed to contain the tetracycline resistance gene, has been identified as IS3. A second genetically characterized insertion sequence (IS1) has been identified as a direct DNA duplication occurring at both junctions of the resistance transfer factor and R-determinant components of R6-5 and related plasmids. A model is presented for the reversible dissociation of resistance transfer factor and R-determinant components of co-integrate R plasmids at the sites of DNA sequence homology provided by the repeated IS regions."}
{"id": "PMID:1092670", "title": "Photosynthetic sulfide oxidation by Chloroflexus aurantiacus, a filamentous, photosynthetic, gliding bacterium.", "content": "Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is related to the green sulfur bacteria (Chlorobiaceae).", "contents": "Photosynthetic sulfide oxidation by Chloroflexus aurantiacus, a filamentous, photosynthetic, gliding bacterium. Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is related to the green sulfur bacteria (Chlorobiaceae)."}
{"id": "PMID:1092671", "title": "Location of the Escherichia coli K-12 ruv gene affecting septum formation after inhibition of deoxyribonucleic acid synthesis.", "content": "Escherichia coli ruv gene was located at 36.1 min on the chromosome by P1 transduction experiments and the gene order his - supD - uvrC, dar4 - ruv - eda - fadD - pps was proposed. Complementation analysis by an F' factor carrying genes in the his region indicated that ultraviolet light sensitivity genes, ruv and uvrC, consist of different cistrons and wild-type alleles of these genes are dominant over the mutant alleles.", "contents": "Location of the Escherichia coli K-12 ruv gene affecting septum formation after inhibition of deoxyribonucleic acid synthesis. Escherichia coli ruv gene was located at 36.1 min on the chromosome by P1 transduction experiments and the gene order his - supD - uvrC, dar4 - ruv - eda - fadD - pps was proposed. Complementation analysis by an F' factor carrying genes in the his region indicated that ultraviolet light sensitivity genes, ruv and uvrC, consist of different cistrons and wild-type alleles of these genes are dominant over the mutant alleles."}
{"id": "PMID:1092672", "title": "A third kasugamycin resistance locus, ksgC, affecting ribosomal protein S2 in Escherichia coli K-12.", "content": "A third kasugamycin-resistant mutant affecting ribosomal protein S2 has been isolated from Escherichia coli K-12. Mating and transduction revealed that this newly recognized kasugamycin resistance locus, designated as ksgC, is located at 0.1 to 0.2 min from purE.", "contents": "A third kasugamycin resistance locus, ksgC, affecting ribosomal protein S2 in Escherichia coli K-12. A third kasugamycin-resistant mutant affecting ribosomal protein S2 has been isolated from Escherichia coli K-12. Mating and transduction revealed that this newly recognized kasugamycin resistance locus, designated as ksgC, is located at 0.1 to 0.2 min from purE."}
{"id": "PMID:1092673", "title": "Biochemical studies on radiation-sensitive mutations in bacteriophage T4-1.", "content": "Mutants of bacteriophage T4 which exhibit increased sensitivity to ultraviolet radiation were isolated and their abilities to induce an enzyme system to excise pyrimidine dimers were examined.  Among 16 mutants isolated, 12 mutants were found to be defective in inducing T4 endonuclease V, which catalyzes the formation of dimer-specific breaks in ultraviolet-irradiated DNA.  A leaky v mutant, which exhibits intermediate ultraviolet sensitivity, was also isolated; the mutant induces a low level of endonuclease V and excises only a small amount of kimers in vitro.  Three other mutants, as well as x and y mutants, were able to induce both T4 endonuclease V and dimer-excision enzyme (5'yields 3' exonuclease).", "contents": "Biochemical studies on radiation-sensitive mutations in bacteriophage T4-1. Mutants of bacteriophage T4 which exhibit increased sensitivity to ultraviolet radiation were isolated and their abilities to induce an enzyme system to excise pyrimidine dimers were examined.  Among 16 mutants isolated, 12 mutants were found to be defective in inducing T4 endonuclease V, which catalyzes the formation of dimer-specific breaks in ultraviolet-irradiated DNA.  A leaky v mutant, which exhibits intermediate ultraviolet sensitivity, was also isolated; the mutant induces a low level of endonuclease V and excises only a small amount of kimers in vitro.  Three other mutants, as well as x and y mutants, were able to induce both T4 endonuclease V and dimer-excision enzyme (5'yields 3' exonuclease)."}
{"id": "PMID:1092674", "title": "Mode of replication of plasmid lambda-dv-1.", "content": "About 20 copies of plasmid lambda-dv are perpetuated per host chromosome in Escherichia coli K12 cells.  The mode of DNA replication of this plasmid lambda-dv was studied, using the density-labelling technique followed by banding in DsCl.  It was shown that all the copies of plasmid lambda-dv are potentially capable of replicating roughly once a cell generation.  Their replication occurs by random, that is a copy of the plasmid is taken out at random for replication from a pool, to which the two replicas resulting from replication are returned.  Chloramphenicol did not inhibit the initiation of a new round of replication of the plasmid molecules, indicating that selection from the replication pool does not require concomitant protein synthesis.", "contents": "Mode of replication of plasmid lambda-dv-1. About 20 copies of plasmid lambda-dv are perpetuated per host chromosome in Escherichia coli K12 cells.  The mode of DNA replication of this plasmid lambda-dv was studied, using the density-labelling technique followed by banding in DsCl.  It was shown that all the copies of plasmid lambda-dv are potentially capable of replicating roughly once a cell generation.  Their replication occurs by random, that is a copy of the plasmid is taken out at random for replication from a pool, to which the two replicas resulting from replication are returned.  Chloramphenicol did not inhibit the initiation of a new round of replication of the plasmid molecules, indicating that selection from the replication pool does not require concomitant protein synthesis."}
{"id": "PMID:1092675", "title": "Isolation and properties of a species produced by the partial dissociation of aspartate transcarbamylase from Escherichia coli.", "content": "A species produced by the reaction of aspartate transcarbamylase (C6R6) with 6 to 12 eq of p-hydroxymercuribenzoate was isolated by DEAE-Sephadex chromatography. Purified material was completely dissociated with mercurials and the relative amounts of catalytic (C) and regulatory (R) subunits were determined by three methods: (a) quantitative cellulose acetate electrophoresis; (b) Lowry analysis after separating the catalytic and regulatory subunits by sucrose gradient centrifugation; (c) dissociation of the species with sodium dodecyl sulfate and determination of the relative amounts of catalytic and regulatory chain by sodium dodecyl sulfate gel electrophoresis. All three methods gave consistent results, indicating that the molecule consists of 75% (by weight) catalytic chain and 25% regulatory chain. The molecular weight determined by gel filtration, sedimentation velocity, and sedimentation equilibrium experiments was found to be approximately 270,000. These observations establish that this species has the structure C6R4, and is produced by the release of a single regulatory dimer R2 from the intact aspartate transcarbamylase complex. This protein (C6R4) contains 20 cysteines and four zinc ions, consistent with the proposed subunit structure. The purified intermediate C6R4 contains no mercury. The parent molecule C6R6 can be reconstituted from C6R4 by incubation with isolated regulatory subunit (R2) in the presence of zinc and beta-mercaptoethanol. Titration of C6R4 yields an end point which corresponds to the addition of 1 mol of regulatory subunit (R2) per mol of C6R4. The intermediate is quite stable at neutral pH but tends to disproportionate into aspartate transcarbamylase and catalytic subunit after prolonged storage or at elevated pH. The kinetic properties of this species have been investigated. The specific activity of C6R4 is virtually identical with that of the native enzyme but the regulatory properties are substantially reduced. Both homotropic and heterotropic interactions are reduced but not abolished, indicating that the intact structure C6R6 is not required for the allosteric transitions involved in regulation.", "contents": "Isolation and properties of a species produced by the partial dissociation of aspartate transcarbamylase from Escherichia coli. A species produced by the reaction of aspartate transcarbamylase (C6R6) with 6 to 12 eq of p-hydroxymercuribenzoate was isolated by DEAE-Sephadex chromatography. Purified material was completely dissociated with mercurials and the relative amounts of catalytic (C) and regulatory (R) subunits were determined by three methods: (a) quantitative cellulose acetate electrophoresis; (b) Lowry analysis after separating the catalytic and regulatory subunits by sucrose gradient centrifugation; (c) dissociation of the species with sodium dodecyl sulfate and determination of the relative amounts of catalytic and regulatory chain by sodium dodecyl sulfate gel electrophoresis. All three methods gave consistent results, indicating that the molecule consists of 75% (by weight) catalytic chain and 25% regulatory chain. The molecular weight determined by gel filtration, sedimentation velocity, and sedimentation equilibrium experiments was found to be approximately 270,000. These observations establish that this species has the structure C6R4, and is produced by the release of a single regulatory dimer R2 from the intact aspartate transcarbamylase complex. This protein (C6R4) contains 20 cysteines and four zinc ions, consistent with the proposed subunit structure. The purified intermediate C6R4 contains no mercury. The parent molecule C6R6 can be reconstituted from C6R4 by incubation with isolated regulatory subunit (R2) in the presence of zinc and beta-mercaptoethanol. Titration of C6R4 yields an end point which corresponds to the addition of 1 mol of regulatory subunit (R2) per mol of C6R4. The intermediate is quite stable at neutral pH but tends to disproportionate into aspartate transcarbamylase and catalytic subunit after prolonged storage or at elevated pH. The kinetic properties of this species have been investigated. The specific activity of C6R4 is virtually identical with that of the native enzyme but the regulatory properties are substantially reduced. Both homotropic and heterotropic interactions are reduced but not abolished, indicating that the intact structure C6R6 is not required for the allosteric transitions involved in regulation."}
{"id": "PMID:1092676", "title": "Interactions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes.", "content": "Bovine and human retinol-retinol-binding protein (RBP) were iodinated to high specific activity. At least 34% of the iodinated material was native and retained its retinol chromophore as judged by its characteristic ability to bind to plasma prealbumin. Bovine and human retinol-125I-RBP were found to bind specifically to intact isolated pigment epithelium cells from bovine eyes. The specific binding was complete in about 1 min with an estimated second order rate constant of about 10-8 M-minus 1 S-minus 1 (at 22 degrees). The specific binding was temperature dependent with the binding at 0 degrees being some 7-fold slower than at 22 degrees. The specific binding of both human and bovine retinol-125I-RBP was a linear function of the number of binding sites (number of cells) and was saturable with respect to retinol-125I-RBP. Bound iodinated retinol-RBP was rapidly displaced by the addition of unlabeled retinol-RBP, indicating that the specific binding process was a surface phenomenon and was not due to endocytosis. At saturation about 3.7 to 5.2 times 10-4 molecules of either bovine or human retinol-125I-RBP bound to one bovine pigment epithelium cell. The dissociation constant for the binding between retinol-125I-RBP and pigment epithelium receptor was estimated to be about 5 times 10-minus 12 M. Addition of human prealbumin (thyroxine-binding prealbumin) did not affect the binding of either human or bovine retinol-125I-RBP to pigment epithelium cells. Retinol-125I-RBP did not bind specifically to isolated bovine rod photoreceptor outer segments. Human apo-RBP was less effective in displacing bound retinol-125I-RBP than either native or reconstituted human retinol-RBP. These results suggest a mechanism whereby, after delivering its retinol to the cell, apo-RBP is displaced from the specific receptor on pigment epithelium cell by another retinol-RBP molecule. This postulated mechanism makes it possible to control the delivery of retinol to the target cell by the relative plasma concentrations of apo- and retinol-RBP and their relative affinities for the specific receptor binding site.", "contents": "Interactions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes. Bovine and human retinol-retinol-binding protein (RBP) were iodinated to high specific activity. At least 34% of the iodinated material was native and retained its retinol chromophore as judged by its characteristic ability to bind to plasma prealbumin. Bovine and human retinol-125I-RBP were found to bind specifically to intact isolated pigment epithelium cells from bovine eyes. The specific binding was complete in about 1 min with an estimated second order rate constant of about 10-8 M-minus 1 S-minus 1 (at 22 degrees). The specific binding was temperature dependent with the binding at 0 degrees being some 7-fold slower than at 22 degrees. The specific binding of both human and bovine retinol-125I-RBP was a linear function of the number of binding sites (number of cells) and was saturable with respect to retinol-125I-RBP. Bound iodinated retinol-RBP was rapidly displaced by the addition of unlabeled retinol-RBP, indicating that the specific binding process was a surface phenomenon and was not due to endocytosis. At saturation about 3.7 to 5.2 times 10-4 molecules of either bovine or human retinol-125I-RBP bound to one bovine pigment epithelium cell. The dissociation constant for the binding between retinol-125I-RBP and pigment epithelium receptor was estimated to be about 5 times 10-minus 12 M. Addition of human prealbumin (thyroxine-binding prealbumin) did not affect the binding of either human or bovine retinol-125I-RBP to pigment epithelium cells. Retinol-125I-RBP did not bind specifically to isolated bovine rod photoreceptor outer segments. Human apo-RBP was less effective in displacing bound retinol-125I-RBP than either native or reconstituted human retinol-RBP. These results suggest a mechanism whereby, after delivering its retinol to the cell, apo-RBP is displaced from the specific receptor on pigment epithelium cell by another retinol-RBP molecule. This postulated mechanism makes it possible to control the delivery of retinol to the target cell by the relative plasma concentrations of apo- and retinol-RBP and their relative affinities for the specific receptor binding site."}
{"id": "PMID:1092677", "title": "Deoxycytidine transport in the presence of a cytidine deaminase inhibitor and the transport of uracil in Escherichia coli B.", "content": "Tetrahydrouridine, a cytidine deaminase inhibitor, prevents periplasmic degradation of deoxycytidine by Escherichia coli B. It does not inhibit deoxycytidine transport and therefore allows an accurate determination of deoxycytidine transport. Data obtained using tetrahydrouridine show that deoxycytidine is transported in E. coli B as the intact nucleoside by an active transport process, with a K-m of 6 times 10-minus 6 M. Cytidine and deoxyadenosine inhibit transport competitively, whereas guanosine has no effect on transport. Arsenate or KCN greatly reduces transport. In a mutant resistant to the nucleoside antibiotic, showdomycin, the active transport of deoxycytidine is lost, and residual slow uptake occurs by passive diffusion. Uracil is accumulated in E. coli B by an active transport process with a K-m of 5 times 10-minus 7 M.", "contents": "Deoxycytidine transport in the presence of a cytidine deaminase inhibitor and the transport of uracil in Escherichia coli B. Tetrahydrouridine, a cytidine deaminase inhibitor, prevents periplasmic degradation of deoxycytidine by Escherichia coli B. It does not inhibit deoxycytidine transport and therefore allows an accurate determination of deoxycytidine transport. Data obtained using tetrahydrouridine show that deoxycytidine is transported in E. coli B as the intact nucleoside by an active transport process, with a K-m of 6 times 10-minus 6 M. Cytidine and deoxyadenosine inhibit transport competitively, whereas guanosine has no effect on transport. Arsenate or KCN greatly reduces transport. In a mutant resistant to the nucleoside antibiotic, showdomycin, the active transport of deoxycytidine is lost, and residual slow uptake occurs by passive diffusion. Uracil is accumulated in E. coli B by an active transport process with a K-m of 5 times 10-minus 7 M."}
{"id": "PMID:1092678", "title": "The sequence of amino acid residues around the oxidation-reduction active disulfide in yeast glutathione reductase.", "content": "A 14-residue peptide containing the oxidation-reduction active cystine residue from yeast glutathione reductase has been isolated from proteolytic digests of the enzyme in which the free sulfhydryl groups had been reacted with N-ethylmaleimide. The sequence of this disulfide-containing peptide was found to be:(see article). The sequence was highly homologous with the active cystine regions in Escherichia coli and pig heart lipoamide dehydrogenase. The sequences of three of the postulated four thiol-containing regions of the enzyme are also presented, as well as evidence supporting the view that the enzyme is composed of two identical subunits.", "contents": "The sequence of amino acid residues around the oxidation-reduction active disulfide in yeast glutathione reductase. A 14-residue peptide containing the oxidation-reduction active cystine residue from yeast glutathione reductase has been isolated from proteolytic digests of the enzyme in which the free sulfhydryl groups had been reacted with N-ethylmaleimide. The sequence of this disulfide-containing peptide was found to be:(see article). The sequence was highly homologous with the active cystine regions in Escherichia coli and pig heart lipoamide dehydrogenase. The sequences of three of the postulated four thiol-containing regions of the enzyme are also presented, as well as evidence supporting the view that the enzyme is composed of two identical subunits."}
{"id": "PMID:1092679", "title": "The mechanism of aminoacylation of transfer ribonucleic acid. Reactivity of enzyme-bound isoleucyl adenylate.", "content": "Isoleucyl adenylate bound to isoleucine:tRNA ligase of Escherichia coli (EC 6.1.1.5; isoleucyl-tRNA synthetase) transfers the isoleucine moiety to tRNA-Ile-E. coli with a half-time of about 35 s at 0 degrees and pH 7.6 in the presence of spermine or Mg2+. If a limited amount of tRNA-Ile is supplied to a mixture of free enzyme and enzyme-bound [14c]isoleucyl adenylate in a medium containing spermine, ATP, and [3H]isoleucine, almost none of the resultant isoleucyl tRNA is derived from preformed enzyme-bound [14C]isoleucyl adenylate. Almost all of the isoleucyl tRNA formed results directly from reaction of free enzyme, ATP, and isoleucine with tRNA. Similar but less clearcut results are obtained when Mg2+ is substituted for spermine. We conclude that isoleucyl adenylate bound to isoleucine:tRNA ligase is not a significant intermediate in the synthesis of isoleucyl tRNA under these conditions.", "contents": "The mechanism of aminoacylation of transfer ribonucleic acid. Reactivity of enzyme-bound isoleucyl adenylate. Isoleucyl adenylate bound to isoleucine:tRNA ligase of Escherichia coli (EC 6.1.1.5; isoleucyl-tRNA synthetase) transfers the isoleucine moiety to tRNA-Ile-E. coli with a half-time of about 35 s at 0 degrees and pH 7.6 in the presence of spermine or Mg2+. If a limited amount of tRNA-Ile is supplied to a mixture of free enzyme and enzyme-bound [14c]isoleucyl adenylate in a medium containing spermine, ATP, and [3H]isoleucine, almost none of the resultant isoleucyl tRNA is derived from preformed enzyme-bound [14C]isoleucyl adenylate. Almost all of the isoleucyl tRNA formed results directly from reaction of free enzyme, ATP, and isoleucine with tRNA. Similar but less clearcut results are obtained when Mg2+ is substituted for spermine. We conclude that isoleucyl adenylate bound to isoleucine:tRNA ligase is not a significant intermediate in the synthesis of isoleucyl tRNA under these conditions."}
{"id": "PMID:1092680", "title": "Cross-linking of Escherichia coli succinic thiokinase. I. Reaction with diiminoesters and dimaleimides.", "content": "Treatment of the tetrameric alpha2 beta2 protein succinic thiokinase of Escherichia coli with dimethylsuberimidate (DMS) yielded five protein species detectable by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These five protein species had estimated molecular weight values of 29,500, 41,000, 73,000, 117,000, and 132,000, and corresponded to alpha monomer, beta monomer, alpha beta dimer, alpha2 beta trimer and alpha2 beta2 tetramer, respectively. In all cases, the cross-linking produced predominantly the 73,000 molecular weight dimer with respectively lesser amounts of the tetramer and trimer. Succinic thiokinase was also cross-linked by reaction with N, N'-o-phynylenedimaleimide or with N, N'-P-phenylenedimaleimide. In these instances, treatment produced the alpha beta dimer as the only oligomeric species. Ammonolysis of isolated tetramer, trimer, and dimer produced by DMS treatment gave the 29,500 molecular weight monomer (alpha monomer) and the 38,500 molecular weight monomer (beta monomer). The absence of dimers of like subunits and the predominance of the dimer of unlike subunits are consistent with a quaternary structure of the native enzyme in which unlike subunits are closely associated but like subunits are not. Under certain conditions, an additional dimer of approximately 60,000 molecular weight was produced. This appeared to result from cleavage of the beta chain of an alpha beta dimer. Phosphorylation of native succinic thiokinase with [gamma-32P]ATP and [gamma-32P]GTP showed radioactivity only in the alpha monomer. Phosphorylation of enzyme before or after cross-linking showed radioactivity in all cross-linked bands except the beta monomer. Experiments in which the enzyme was titrated with [14C]DMS and trinitrobenzenesulfonate revealed that approximately half of the available amino groups reacted with the diiminoester, but that a small fraction of these (smaller than 20%) had reacted bifunctionally", "contents": "Cross-linking of Escherichia coli succinic thiokinase. I. Reaction with diiminoesters and dimaleimides. Treatment of the tetrameric alpha2 beta2 protein succinic thiokinase of Escherichia coli with dimethylsuberimidate (DMS) yielded five protein species detectable by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These five protein species had estimated molecular weight values of 29,500, 41,000, 73,000, 117,000, and 132,000, and corresponded to alpha monomer, beta monomer, alpha beta dimer, alpha2 beta trimer and alpha2 beta2 tetramer, respectively. In all cases, the cross-linking produced predominantly the 73,000 molecular weight dimer with respectively lesser amounts of the tetramer and trimer. Succinic thiokinase was also cross-linked by reaction with N, N'-o-phynylenedimaleimide or with N, N'-P-phenylenedimaleimide. In these instances, treatment produced the alpha beta dimer as the only oligomeric species. Ammonolysis of isolated tetramer, trimer, and dimer produced by DMS treatment gave the 29,500 molecular weight monomer (alpha monomer) and the 38,500 molecular weight monomer (beta monomer). The absence of dimers of like subunits and the predominance of the dimer of unlike subunits are consistent with a quaternary structure of the native enzyme in which unlike subunits are closely associated but like subunits are not. Under certain conditions, an additional dimer of approximately 60,000 molecular weight was produced. This appeared to result from cleavage of the beta chain of an alpha beta dimer. Phosphorylation of native succinic thiokinase with [gamma-32P]ATP and [gamma-32P]GTP showed radioactivity only in the alpha monomer. Phosphorylation of enzyme before or after cross-linking showed radioactivity in all cross-linked bands except the beta monomer. Experiments in which the enzyme was titrated with [14C]DMS and trinitrobenzenesulfonate revealed that approximately half of the available amino groups reacted with the diiminoester, but that a small fraction of these (smaller than 20%) had reacted bifunctionally"}
{"id": "PMID:1092681", "title": "Purification and properties of 7, 8-diaminopelargonic acid aminotransferase.", "content": "The enzyme 7, 8-diaminopelargonic acid aminotransferase utilizes S-adenosyl-L-methionine to transaminate the biotin precurson 7-keto-8-aminopelargonic acid and form the next intermediate in the pathway, 7, 8-diaminopelargonic acid. The enzyme has been purified nearly 1000-fold from an extract of a regulatory mutant of Escherichia coli which is derepressed for the enzymes of the biotin operon. The extract was treated with protamine sulfate, ammonium sulfate, and subjected to acid and heat treatments. Subsequently, the enzyme was chromatographed on columns of DEAE-cellulose, phosphocellulose, hydroxylapatite, and two Sephadex G-100. The resulting purified preparation was judged 86% homogeneous by the scanning of of a stained disc gel. The enzymatic activity was associated with the major band in gels run at two different gel concentrations and two different pH values. The cofactor, pyridoxal phosphate, can be resolved from the enzyme in the presence of phosphate buffer after incubation with the amino donor, S-adenosyl-L-methionine. A molecular weight estimation of 94,000 plus or minus 10, 000 has been obtained by gel filtration and sucrose gradient sedimentation studies. Gel electrophoresis in the presence of sodium dodecyl sulfate, shows a single subunit with a molecular weight of 47, 000 plus or minus 3, 000 indicating a dimeric enzyme. A neutral compound was detected in the acidified reaction mixture which was derived from the methionine moiety of S-adenosyl-L-methionine and was present in amounts equivalent to the 7, 8-diaminopelargonic acid produced in the reaction mixture. It is suggested that the keto product of the reaction, i.e. S-adenosyl-2-oxo-4-methylthiobutyric acid, may decompose nonenzymatically under the conditions of the reaction to form 5'-methylthioadenosine and the neutral compound, 2-oxo-3-butenoic acid.", "contents": "Purification and properties of 7, 8-diaminopelargonic acid aminotransferase. The enzyme 7, 8-diaminopelargonic acid aminotransferase utilizes S-adenosyl-L-methionine to transaminate the biotin precurson 7-keto-8-aminopelargonic acid and form the next intermediate in the pathway, 7, 8-diaminopelargonic acid. The enzyme has been purified nearly 1000-fold from an extract of a regulatory mutant of Escherichia coli which is derepressed for the enzymes of the biotin operon. The extract was treated with protamine sulfate, ammonium sulfate, and subjected to acid and heat treatments. Subsequently, the enzyme was chromatographed on columns of DEAE-cellulose, phosphocellulose, hydroxylapatite, and two Sephadex G-100. The resulting purified preparation was judged 86% homogeneous by the scanning of of a stained disc gel. The enzymatic activity was associated with the major band in gels run at two different gel concentrations and two different pH values. The cofactor, pyridoxal phosphate, can be resolved from the enzyme in the presence of phosphate buffer after incubation with the amino donor, S-adenosyl-L-methionine. A molecular weight estimation of 94,000 plus or minus 10, 000 has been obtained by gel filtration and sucrose gradient sedimentation studies. Gel electrophoresis in the presence of sodium dodecyl sulfate, shows a single subunit with a molecular weight of 47, 000 plus or minus 3, 000 indicating a dimeric enzyme. A neutral compound was detected in the acidified reaction mixture which was derived from the methionine moiety of S-adenosyl-L-methionine and was present in amounts equivalent to the 7, 8-diaminopelargonic acid produced in the reaction mixture. It is suggested that the keto product of the reaction, i.e. S-adenosyl-2-oxo-4-methylthiobutyric acid, may decompose nonenzymatically under the conditions of the reaction to form 5'-methylthioadenosine and the neutral compound, 2-oxo-3-butenoic acid."}
{"id": "PMID:1092682", "title": "Biosynthesis of 7, 8-diaminopelargonic acid from  7-keto-8-aminopelargonic acid and S-adenosyl-L-methionine. The kinetics of the reaction.", "content": "The transamination of 7-keto-8-aminopelargonic acid by 7, 8-diaminopelargonic acid aminotransferase of Escherichia coli requires S-adenosyl-L-methionine as the amino donor. Initial velocity studies of this reaction revealed a parallel pattern of reciprocal plots characteristic of a ping-pong mechanism. m-Keto-8-aminopelargonic acid showed strong substrate inhibition which was competitive with S-adenosyl-L-methionine. The Michaelis constants determined for S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid were 0.20 mM and 1.2 muM, respectively. The Vmax of 0.16 mumol/mg/min corresponds to a turnover number for the enzyme of only 17 molecules/molecule enzyme/min. The Km values for the interaction of pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate with the apoenzyme were determined to be 32 muM and 21 muM, respectively. Two classes of inhibitors were observed: (a) those which showed competitive inhibition with respect to S-adenosynd (b) those which showed noncompetitive inhibition with respect to both substrates. In the former group were S-adenosyl-L-(2-hydroxy-4-methylthio)butyric acid and adenosine. In the latter were S-adenosyl-L-ethionine, adenine, and 8-keto-7-aminopelargonic acid. L-Methionine, S-methyl-L-methionine, inosine, and hypoxanthine were not significantly inhibitory. Certain conformations of the substrates in the active site of the enzyme have been proposed which explain: (a) the requirement for the sulfonium ion of S-adenosyl-L-methionine for activity but not for binding to the enzyme, and (b) the ability of 7-keto-8-aminopelargonic acid to bind to the pyridoxal form of the enzyme as a potent substrate inhibitor.", "contents": "Biosynthesis of 7, 8-diaminopelargonic acid from  7-keto-8-aminopelargonic acid and S-adenosyl-L-methionine. The kinetics of the reaction. The transamination of 7-keto-8-aminopelargonic acid by 7, 8-diaminopelargonic acid aminotransferase of Escherichia coli requires S-adenosyl-L-methionine as the amino donor. Initial velocity studies of this reaction revealed a parallel pattern of reciprocal plots characteristic of a ping-pong mechanism. m-Keto-8-aminopelargonic acid showed strong substrate inhibition which was competitive with S-adenosyl-L-methionine. The Michaelis constants determined for S-adenosyl-L-methionine and 7-keto-8-aminopelargonic acid were 0.20 mM and 1.2 muM, respectively. The Vmax of 0.16 mumol/mg/min corresponds to a turnover number for the enzyme of only 17 molecules/molecule enzyme/min. The Km values for the interaction of pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate with the apoenzyme were determined to be 32 muM and 21 muM, respectively. Two classes of inhibitors were observed: (a) those which showed competitive inhibition with respect to S-adenosynd (b) those which showed noncompetitive inhibition with respect to both substrates. In the former group were S-adenosyl-L-(2-hydroxy-4-methylthio)butyric acid and adenosine. In the latter were S-adenosyl-L-ethionine, adenine, and 8-keto-7-aminopelargonic acid. L-Methionine, S-methyl-L-methionine, inosine, and hypoxanthine were not significantly inhibitory. Certain conformations of the substrates in the active site of the enzyme have been proposed which explain: (a) the requirement for the sulfonium ion of S-adenosyl-L-methionine for activity but not for binding to the enzyme, and (b) the ability of 7-keto-8-aminopelargonic acid to bind to the pyridoxal form of the enzyme as a potent substrate inhibitor."}
{"id": "PMID:1092683", "title": "The steady state kinetic parameters and non-processivity of Escherichia coli deoxyribonucleic acid polymerase I.", "content": "A steady state kinetic study of Escherichia coli DNA polymerase I has been carried out using poly[d(A-T)] as the template-primer substrate. The results of substrate saturation and product inhibition kinetic studies suggest an altered Ordered Bi Bi mechanism for the enzyme. The Michaelis constants for polymer, d-atp, and dTTP are 5 nM (3'-OH ends), 1 muM, and 2 muM, respectively. The apparent equilibrium constant for the reaction, Keq equals [PPi]/[dNTP], was estimated as greater than or equal to 500. No quaternary complex of enzyme, template, and both deoxynucleoside triphosphates was detected. Single turnover experiments at 4 degrees indicated that the enzyme functions non-processively under the specified conditions, that is, dissociates after each catalytic step. The results at higher temperature were consistent with dissociation within 30 steps. Furthermore, at 4 degrees a burst of incorporation stoichiometric with the amount of enzyme was observed upon initiation of the reaction, indicating that the rate-limiting step in the steady state occurs after phosphodiester bond formation. There is a linear Arrhenius dependence of the initial reaction on temperature in the range 4-40 degrees, with an apparent Ea equals 17 kcal/mol. The rate equations appropriate for template-dependent polymerases which dissociate after each catalytic step have been derived.", "contents": "The steady state kinetic parameters and non-processivity of Escherichia coli deoxyribonucleic acid polymerase I. A steady state kinetic study of Escherichia coli DNA polymerase I has been carried out using poly[d(A-T)] as the template-primer substrate. The results of substrate saturation and product inhibition kinetic studies suggest an altered Ordered Bi Bi mechanism for the enzyme. The Michaelis constants for polymer, d-atp, and dTTP are 5 nM (3'-OH ends), 1 muM, and 2 muM, respectively. The apparent equilibrium constant for the reaction, Keq equals [PPi]/[dNTP], was estimated as greater than or equal to 500. No quaternary complex of enzyme, template, and both deoxynucleoside triphosphates was detected. Single turnover experiments at 4 degrees indicated that the enzyme functions non-processively under the specified conditions, that is, dissociates after each catalytic step. The results at higher temperature were consistent with dissociation within 30 steps. Furthermore, at 4 degrees a burst of incorporation stoichiometric with the amount of enzyme was observed upon initiation of the reaction, indicating that the rate-limiting step in the steady state occurs after phosphodiester bond formation. There is a linear Arrhenius dependence of the initial reaction on temperature in the range 4-40 degrees, with an apparent Ea equals 17 kcal/mol. The rate equations appropriate for template-dependent polymerases which dissociate after each catalytic step have been derived."}
{"id": "PMID:1092684", "title": "Characterization of fragments of 16 S ribonucleic acid protected against pancreatic ribonuclease digestion by ribosomal protein S4.", "content": "Digestion of reconstituted complexes of 16 S RNA and ribosomal protein S4 with pancreatic ribonuclease, followed by resolution of the products on polyacrylamide gels of low ionic strength, gives rise to a protected ribonucleoprotein fragment with a mobility of 7 S. Fingerprinting of the RNA moiety indicates the presence of sequences covering the 5' one-third of the 16 S RNA. After its extraction from the acrylamide gels, the protected RNA from the complex can be separated into two components by sedimentation in sucrose gradients. One of these retains the ability to reassociate specifically with S4, as judged from its stoichiometry of binding in the presence of excess S4 and from its selection of S4 alone from a mixture of unfractionated 30S ribosomal proteins. It spans the same regions of the 16 S RNA as the total protected RNA, but lacks an internal sequence of about 120 nucleotides. This component contains a number of nicks which give rise to discrete bands upon electrophoresis in urea. The principal subfragments include Sections L through F (230 nucleotides), B through I'' (160 nucleotides), and part of section C'' (35 to 40 nucleotides). It is proposed that the binding site for S4 lies within these noncontiguous sequences, in support of the hypothesis that S4 makes multiple contacts with the 16 S RNA.", "contents": "Characterization of fragments of 16 S ribonucleic acid protected against pancreatic ribonuclease digestion by ribosomal protein S4. Digestion of reconstituted complexes of 16 S RNA and ribosomal protein S4 with pancreatic ribonuclease, followed by resolution of the products on polyacrylamide gels of low ionic strength, gives rise to a protected ribonucleoprotein fragment with a mobility of 7 S. Fingerprinting of the RNA moiety indicates the presence of sequences covering the 5' one-third of the 16 S RNA. After its extraction from the acrylamide gels, the protected RNA from the complex can be separated into two components by sedimentation in sucrose gradients. One of these retains the ability to reassociate specifically with S4, as judged from its stoichiometry of binding in the presence of excess S4 and from its selection of S4 alone from a mixture of unfractionated 30S ribosomal proteins. It spans the same regions of the 16 S RNA as the total protected RNA, but lacks an internal sequence of about 120 nucleotides. This component contains a number of nicks which give rise to discrete bands upon electrophoresis in urea. The principal subfragments include Sections L through F (230 nucleotides), B through I'' (160 nucleotides), and part of section C'' (35 to 40 nucleotides). It is proposed that the binding site for S4 lies within these noncontiguous sequences, in support of the hypothesis that S4 makes multiple contacts with the 16 S RNA."}
{"id": "PMID:1092685", "title": "The role of S-adenosylmethionine in the cleavage of deoxyribonucleic acid by the restriction endonuclease from Escherichia coli K.", "content": "The restriction endonuclease from Escherichia coli K specifically cleaves foreign DNA in the presence of S-adenosylmethionine, ATP, and Mg2+. The role of S-adenosylmethionine in this reaction has been studied by following the specific binding of the enzyme to unmodified DNA. The results indicate that S-adenosylmethionine acts as an allosteric effector. However, the rate-limiting step in the activation of the enzyme is not the binding of the effector itself, but an event subsequent to it. The interaction of the S-adenosylmethionine with two mutant K restriction endonucleases isolated previously has also been investigated. One of them, which is defective in restriction, can be activated in a manner similar to the wild type enzyme, while the other one, which lacks both restriction and modification activities (due to a mutation in the subunit responsible for DNA recognition), shows no such effect.", "contents": "The role of S-adenosylmethionine in the cleavage of deoxyribonucleic acid by the restriction endonuclease from Escherichia coli K. The restriction endonuclease from Escherichia coli K specifically cleaves foreign DNA in the presence of S-adenosylmethionine, ATP, and Mg2+. The role of S-adenosylmethionine in this reaction has been studied by following the specific binding of the enzyme to unmodified DNA. The results indicate that S-adenosylmethionine acts as an allosteric effector. However, the rate-limiting step in the activation of the enzyme is not the binding of the effector itself, but an event subsequent to it. The interaction of the S-adenosylmethionine with two mutant K restriction endonucleases isolated previously has also been investigated. One of them, which is defective in restriction, can be activated in a manner similar to the wild type enzyme, while the other one, which lacks both restriction and modification activities (due to a mutation in the subunit responsible for DNA recognition), shows no such effect."}
{"id": "PMID:1092686", "title": "Nucleotide sequence determination of bacteriophage T4 species I ribonucleic acid.", "content": "The nucleotide sequence of T4 species I RNA, one of several stable RNA's specifically coded for by bacteriophage T4, has been determined using 32-P-labeled material from T4-infected cultures of Escherichia coli. The purified RNA species which has been sequenced has been shown to hybridize well to T4 DNA (Wilson J.H., Kim, J.S., and Abelson, J.N. (1972) J. Mol. Biol. 71, 547-556). The sequence is: pCGAUUCGAGGAAAUAUCUUUGCCGUAAGCCGAGUAGCGUUUUUGACGGAACGUUCGGAUAUGGUUGAGAUAUGGCCUUUUAAAAUAUUGAGUAGCGUCAACUACUUAAUAACCGGGUUCGAAUCCCGGCGUUUCGU-CAA-OHACA-OH. Species I RNA which is 140 nucleotides long is also found to occur in shorter versions with 135 to 136 nucleotides which terminate with a 3'-phosphate. The molecule can be arranged in a secondary structure which shows some striking similarities to the classic cloverleaf pattern of a tRNA. The molecule is specifically cleaved by an E. coli nuclease into three segments by cleavage at a double-stranded region in the molecule. The function of species I RNA is unknown, but evidence presented elsewhere (Paddock, G.V., and Abelson, J. (1975) J. Biol. Chem. 250, 4207-4219) indicates that the gene for this RNA molecule has been preserved in evolution. The position of a mutation within species I RNA has been determined. This mutation results in incorrect processing of the RNA and lower relative yields of the RNA are present.", "contents": "Nucleotide sequence determination of bacteriophage T4 species I ribonucleic acid. The nucleotide sequence of T4 species I RNA, one of several stable RNA's specifically coded for by bacteriophage T4, has been determined using 32-P-labeled material from T4-infected cultures of Escherichia coli. The purified RNA species which has been sequenced has been shown to hybridize well to T4 DNA (Wilson J.H., Kim, J.S., and Abelson, J.N. (1972) J. Mol. Biol. 71, 547-556). The sequence is: pCGAUUCGAGGAAAUAUCUUUGCCGUAAGCCGAGUAGCGUUUUUGACGGAACGUUCGGAUAUGGUUGAGAUAUGGCCUUUUAAAAUAUUGAGUAGCGUCAACUACUUAAUAACCGGGUUCGAAUCCCGGCGUUUCGU-CAA-OHACA-OH. Species I RNA which is 140 nucleotides long is also found to occur in shorter versions with 135 to 136 nucleotides which terminate with a 3'-phosphate. The molecule can be arranged in a secondary structure which shows some striking similarities to the classic cloverleaf pattern of a tRNA. The molecule is specifically cleaved by an E. coli nuclease into three segments by cleavage at a double-stranded region in the molecule. The function of species I RNA is unknown, but evidence presented elsewhere (Paddock, G.V., and Abelson, J. (1975) J. Biol. Chem. 250, 4207-4219) indicates that the gene for this RNA molecule has been preserved in evolution. The position of a mutation within species I RNA has been determined. This mutation results in incorrect processing of the RNA and lower relative yields of the RNA are present."}
{"id": "PMID:1092687", "title": "Nucleotide sequence determination of bacteriophage T2 and T6 species I ribonucleic acids.", "content": "The nucleotide sequences of species I RNA coded for by bacteriophages T2 and T6 have been analyzed using 32-P-labeled material from T2 and T6-infected cultures of Escherichia coli. The T1 and pancreatic ribonuclease digestion products were partially analyzed and the results were compared with nucleotide sequences from T4 species I RNA to obtain a minimum estimate of the number of nucleotide sequence differences among the three species I RNAs. Analysis of fragments obtained by digestion with epsilon-carboxymethyl-lysine-41-pancreatic ribonuclease and with E. coli Q13 S30 crude extract was also performed to provide some additional confirmation for the nucleotide sequences that were derived for the T2 and T6 species I RNAs. T2 species I RNA was found to be different at three positions in the nucleotide sequence, and unlike T4 species I RNA, contained in addition the modified nucleotide, psi, in a region where the proposed secondary structure is identical to the TpsiC-loop of a tRNA. T6 species I RNA was found to contain nucleotide differences from the T4 species I RNA sequence at four positions. The U at position 119 in the sequence appears to be modified to psi only to a small extent. While a biological function for species I RNA is unknown, the fact that there is over 97% homology in the sequences suggests strong evolutionary pressures to retain the nucleotide sequence in the T-even genomes.", "contents": "Nucleotide sequence determination of bacteriophage T2 and T6 species I ribonucleic acids. The nucleotide sequences of species I RNA coded for by bacteriophages T2 and T6 have been analyzed using 32-P-labeled material from T2 and T6-infected cultures of Escherichia coli. The T1 and pancreatic ribonuclease digestion products were partially analyzed and the results were compared with nucleotide sequences from T4 species I RNA to obtain a minimum estimate of the number of nucleotide sequence differences among the three species I RNAs. Analysis of fragments obtained by digestion with epsilon-carboxymethyl-lysine-41-pancreatic ribonuclease and with E. coli Q13 S30 crude extract was also performed to provide some additional confirmation for the nucleotide sequences that were derived for the T2 and T6 species I RNAs. T2 species I RNA was found to be different at three positions in the nucleotide sequence, and unlike T4 species I RNA, contained in addition the modified nucleotide, psi, in a region where the proposed secondary structure is identical to the TpsiC-loop of a tRNA. T6 species I RNA was found to contain nucleotide differences from the T4 species I RNA sequence at four positions. The U at position 119 in the sequence appears to be modified to psi only to a small extent. While a biological function for species I RNA is unknown, the fact that there is over 97% homology in the sequences suggests strong evolutionary pressures to retain the nucleotide sequence in the T-even genomes."}
{"id": "PMID:1092688", "title": "Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles.", "content": "In the preceding paper the preparation and characterization of antiserum to purified D-lactate are described. In this paper the effects of the antibody on D-lactate dehydrogenase activity and D-lactate-dependent active transport in native Escherichia coli ML 308-225 membrane vesicles and ML 308-225dld-3 vesicles reconstituted with D-lactate dehydrogenase are described. The results demonstrate that D-lactate dehydrogenase is inaccessible to antibody in native ML 308-225 vesicles, but readily accessible to antibody in reconstituted dld-3 vesicles. The findings indicate that D-lactate dehydrogenase is located on the inner surface of native ML 308-225 vesicles and on the outer surface of reconstituted dld-3 vesicles. The results with the native vesicle preparations also provide further evidence that virtually none of the vesicles is inverted or sufficiently damaged to allow access of antibody to D-lactate dehydrogenase. In addition, experiments are presented which demonstrate that an impermeable electron carrier, reduced 5-N-methylphenazonium-3-sulfonate, drives active transport in native ML 308-225 vesicles as well as its permeable analogue reduced phenazine methosulfate. Thus, reduction of the respiratory chain from either side of the vesicle membrane is able to drive active transport. Ca2+, Mg2+-stimulated ATPase is also inaccessible to antibody in ML 308-225 vesicles unless the preparation is subjected to ultrasonic sound, incubated in Tris buffer at pH 9.0, or homogenized vigorously. Moreover, as opposed to D-lactate dehydrogenase and cytochrome b1, ATPase is readily lost from the membrane during the preparation of vesicles.", "contents": "Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. In the preceding paper the preparation and characterization of antiserum to purified D-lactate are described. In this paper the effects of the antibody on D-lactate dehydrogenase activity and D-lactate-dependent active transport in native Escherichia coli ML 308-225 membrane vesicles and ML 308-225dld-3 vesicles reconstituted with D-lactate dehydrogenase are described. The results demonstrate that D-lactate dehydrogenase is inaccessible to antibody in native ML 308-225 vesicles, but readily accessible to antibody in reconstituted dld-3 vesicles. The findings indicate that D-lactate dehydrogenase is located on the inner surface of native ML 308-225 vesicles and on the outer surface of reconstituted dld-3 vesicles. The results with the native vesicle preparations also provide further evidence that virtually none of the vesicles is inverted or sufficiently damaged to allow access of antibody to D-lactate dehydrogenase. In addition, experiments are presented which demonstrate that an impermeable electron carrier, reduced 5-N-methylphenazonium-3-sulfonate, drives active transport in native ML 308-225 vesicles as well as its permeable analogue reduced phenazine methosulfate. Thus, reduction of the respiratory chain from either side of the vesicle membrane is able to drive active transport. Ca2+, Mg2+-stimulated ATPase is also inaccessible to antibody in ML 308-225 vesicles unless the preparation is subjected to ultrasonic sound, incubated in Tris buffer at pH 9.0, or homogenized vigorously. Moreover, as opposed to D-lactate dehydrogenase and cytochrome b1, ATPase is readily lost from the membrane during the preparation of vesicles."}
{"id": "PMID:1092689", "title": "Interaction of deoxycholate and of detergents with the coat protein of bacteriophage f1.", "content": "The major coat protein of bacteriophage f1, which is localized in the host membrane during phage maturation, has a hydrophobic binding site capable of binding deoxycholate and a variety of detergents to form a soluble particle, and in that respect, resembles many membrane proteins. The soluble particle has properties that suggest it is formed by simple insertion of protein into a deoxycholate or detergent micelle, but molecular weight measurements show that the protein is present as a dimer, even in sodium dodecyl sulfate, indicating the existence of unusually strong forces for self-association. A by-product of the investigation has been to show that detergents can be very helpful in the fractionation of the constituent molecules of the virus: deoxycholate-solubilized virus is readily fractionated by gel chromatography into DNA, A protein, and B protein, with virtually no cross-contamination.", "contents": "Interaction of deoxycholate and of detergents with the coat protein of bacteriophage f1. The major coat protein of bacteriophage f1, which is localized in the host membrane during phage maturation, has a hydrophobic binding site capable of binding deoxycholate and a variety of detergents to form a soluble particle, and in that respect, resembles many membrane proteins. The soluble particle has properties that suggest it is formed by simple insertion of protein into a deoxycholate or detergent micelle, but molecular weight measurements show that the protein is present as a dimer, even in sodium dodecyl sulfate, indicating the existence of unusually strong forces for self-association. A by-product of the investigation has been to show that detergents can be very helpful in the fractionation of the constituent molecules of the virus: deoxycholate-solubilized virus is readily fractionated by gel chromatography into DNA, A protein, and B protein, with virtually no cross-contamination."}
{"id": "PMID:1092693", "title": "A historical review of the histology of patent autogenous vein grafts and vein patches.", "content": "The literature on the histological changes occurring in autogenous vein grafts and patches was reviewed. Over 307 grafts (235 animal and 72 human) and 30 patches (28 animal and 2 human) have been described. Grafts were investigated from patients who died on the operating table up to 22 years after operation. Intimal thickening and various changes in the media (including necrosis and replacement fibrosis) occurred. Dilatation of vein grafts has occurred, and anerysm formation has been reported. Atheroma has been described in the grafts from 5 patients.", "contents": "A historical review of the histology of patent autogenous vein grafts and vein patches. The literature on the histological changes occurring in autogenous vein grafts and patches was reviewed. Over 307 grafts (235 animal and 72 human) and 30 patches (28 animal and 2 human) have been described. Grafts were investigated from patients who died on the operating table up to 22 years after operation. Intimal thickening and various changes in the media (including necrosis and replacement fibrosis) occurred. Dilatation of vein grafts has occurred, and anerysm formation has been reported. Atheroma has been described in the grafts from 5 patients."}
{"id": "PMID:1092694", "title": "Operations for lymphoedema of the lower limbs. A study of the results in 108 operations utilizing vascularized dermal flaps.", "content": "The results of operations for lymphoedema of the lower limbs have been reviewed. In experienced hands the results are very satisfactory. Modifications of the buried dermis flap operation of Thompson have been found to give the best results in patients where the local skin has been in good condition. The chief complication has been the occurrence of localized areas of necrosis, chiefly at the edges of the flaps. It has not been a serious complication but has sometimes prolonged the stay in hospital. It has been associated with haematoma formation. Methods of avoiding this and other complications are discussed in order to improve results still further. There have been no serious complications and no deaths in this series.", "contents": "Operations for lymphoedema of the lower limbs. A study of the results in 108 operations utilizing vascularized dermal flaps. The results of operations for lymphoedema of the lower limbs have been reviewed. In experienced hands the results are very satisfactory. Modifications of the buried dermis flap operation of Thompson have been found to give the best results in patients where the local skin has been in good condition. The chief complication has been the occurrence of localized areas of necrosis, chiefly at the edges of the flaps. It has not been a serious complication but has sometimes prolonged the stay in hospital. It has been associated with haematoma formation. Methods of avoiding this and other complications are discussed in order to improve results still further. There have been no serious complications and no deaths in this series."}
{"id": "PMID:1092698", "title": "Cytoplasmic type 80S ribosomes associated with yeast mitochondria. IV. Attachment of ribosomes to the outer membrane of isolated mitochondria.", "content": "Growing yeast spheroplasts were shown to have, on the average, four times the number of cytoplasmic ribosomes in contact with the outer mitochondrial membrane compared to starved spheroplasts. Ribosomes in contact with mitochondria in the growing spheroplast preparation, like free cytoplasmic ribosomes, exist primarily as polysome structures. In the starved spheroplast preparation, both mitochondria-bound and free cytoplasmic ribosomes exist primarily as monosomes. Mitochondria isolated from growing spheroplasts in a medium containing lmM Mg++ have cytoplasmic ribosomes bound directly to the outer membrane. These ribosomes can be quantitatively removed by washing the mitochondria with 2 mM EDTA. Mitochondria from starved spheroplasts are capable of accepting either free cytoplasmic polysomes or cytoplasmic polysomes extracted from mitochondria. However, the extent of polysome binding to mitochondria was shown to be a direct function of the Mg++ concentration; a smaller percentage of the input polysomes bind as the Mg++ concentration is lowered. At 1 mM Mg++, neither free cytoplasmic nor mitochondria-bound polysomes bind to mitochondria. Nevertheless, when growing spheroplasts are broken and mitochondria isolated in medium containing 1 mM Mg++, the mitochondria are seen to have cytoplasmic ribosomes firmly attached to the outer membrane. This result, in addition to our earlier data (Kellems, R. E., and R. A. Butow. 1974. J. Biol. Chem. 249:3304-3310), support the view that cytoplasmic ribosomes attached to the outer membrane of purified mitochondria were attached in vivo. In preparations of mitochondria isolated from growing spheroplasts, ribosomes appear to be found to specific regions of the outer membrane, namely those regions which are in close association or in contact with the inner mitochondrial membrane. This is particularly evident with mitochondria in a condensed configuration. This finding suggests a mechanism whereby cytoplasmically synthesized mitochondrial protein could be transferred by a process of vectorial translation across both membranes of the organelle.", "contents": "Cytoplasmic type 80S ribosomes associated with yeast mitochondria. IV. Attachment of ribosomes to the outer membrane of isolated mitochondria. Growing yeast spheroplasts were shown to have, on the average, four times the number of cytoplasmic ribosomes in contact with the outer mitochondrial membrane compared to starved spheroplasts. Ribosomes in contact with mitochondria in the growing spheroplast preparation, like free cytoplasmic ribosomes, exist primarily as polysome structures. In the starved spheroplast preparation, both mitochondria-bound and free cytoplasmic ribosomes exist primarily as monosomes. Mitochondria isolated from growing spheroplasts in a medium containing lmM Mg++ have cytoplasmic ribosomes bound directly to the outer membrane. These ribosomes can be quantitatively removed by washing the mitochondria with 2 mM EDTA. Mitochondria from starved spheroplasts are capable of accepting either free cytoplasmic polysomes or cytoplasmic polysomes extracted from mitochondria. However, the extent of polysome binding to mitochondria was shown to be a direct function of the Mg++ concentration; a smaller percentage of the input polysomes bind as the Mg++ concentration is lowered. At 1 mM Mg++, neither free cytoplasmic nor mitochondria-bound polysomes bind to mitochondria. Nevertheless, when growing spheroplasts are broken and mitochondria isolated in medium containing 1 mM Mg++, the mitochondria are seen to have cytoplasmic ribosomes firmly attached to the outer membrane. This result, in addition to our earlier data (Kellems, R. E., and R. A. Butow. 1974. J. Biol. Chem. 249:3304-3310), support the view that cytoplasmic ribosomes attached to the outer membrane of purified mitochondria were attached in vivo. In preparations of mitochondria isolated from growing spheroplasts, ribosomes appear to be found to specific regions of the outer membrane, namely those regions which are in close association or in contact with the inner mitochondrial membrane. This is particularly evident with mitochondria in a condensed configuration. This finding suggests a mechanism whereby cytoplasmically synthesized mitochondrial protein could be transferred by a process of vectorial translation across both membranes of the organelle."}
{"id": "PMID:1092697", "title": "Simplified technique for the insertion of indwelling catheters in the pulmonary artery and left atrium.", "content": "A technique for the insertion of Telfon catheters into the pulmonary artery and left atrium is described. The method is especially useful for continous monitoring of pulmonary artery and left atrial pressures and sampling of mixed venous and arterial blood after open or closed heart surgery. No cpmplication associated with this technique have occurred in more than 100 clinical cases.", "contents": "Simplified technique for the insertion of indwelling catheters in the pulmonary artery and left atrium. A technique for the insertion of Telfon catheters into the pulmonary artery and left atrium is described. The method is especially useful for continous monitoring of pulmonary artery and left atrial pressures and sampling of mixed venous and arterial blood after open or closed heart surgery. No cpmplication associated with this technique have occurred in more than 100 clinical cases."}
{"id": "PMID:1092699", "title": "Concanavalin A receptors, immunoglobulins, and theta antigen of the lymphocyte surface. Interactions with concanavalin A and with Cytoplasmic structures.", "content": "The effect of concanavalin A (Con A) on the capping of mouse lymphocyte surface immunoglobulin (surface Ig), cross-linked by rabbit anti-mouse Ig antibody, and on the capping of mouse thymocyte theta antigen, cross-linked by anti-theta alloantibody and rabbit anti-mouse Ig antibody, has been studied by immunofluorescence, using fluorescein conjugated Con A and rhodamine-conjugated anti-mouse Ig antibody, and by electron microscopy, using native or fluorescein-conjugated Con A and ferritin-conjugated anti-mouse Ig antibody. Prior incubation of the cells with Con A inhibited only partially capping os surface Ig, whereas it blocked almost completely capping of theta antigens. Both on cells with rings and on cells with caps the staining for surface Ig or theta antigen was superimposed to the staining for Con A. When Con A receptors on spleen cells were capped by Con A at concentrations of 10 mug/ml or higher, and the distribution of surface Ig was examined under noncapping conditions, all detectable surface Ig were found in the caps. As shown by electron microscopy, surface Ig remained dispersed in a layer of Con A. The ability of Con A to cap surface Ig was not altered by the presence of cohchicine or vinblastine. These results suggest that surface Ig are cross-linked by Con A to other Con A receptors. In these conditions surface Ig behave essentially as Con A receptors, as for example, in their sensitivity to cytochalasin B during inhibition or reversal of capping induced by this drug. The behavior of surface Ig parallels that of Con A receptors also in the presence of vinblastine. It is concluded that in the presence of Con A, antimitotic drugs do not modify directly the interaction between Con A receptors and surface Ig, but probably influence the capping ability of the Con A receptors or, more in general, affect the ability to elicit movements over the cell surface. The role in capping of cytochalasin-sensitive and vinblastine-sensitive structures is discussed. Both types of structures appear to play an active role in the formation of a cap, although the former probably corresponds to the main mechanical system responsible for the active displacement of cytoplasmic and surface material.", "contents": "Concanavalin A receptors, immunoglobulins, and theta antigen of the lymphocyte surface. Interactions with concanavalin A and with Cytoplasmic structures. The effect of concanavalin A (Con A) on the capping of mouse lymphocyte surface immunoglobulin (surface Ig), cross-linked by rabbit anti-mouse Ig antibody, and on the capping of mouse thymocyte theta antigen, cross-linked by anti-theta alloantibody and rabbit anti-mouse Ig antibody, has been studied by immunofluorescence, using fluorescein conjugated Con A and rhodamine-conjugated anti-mouse Ig antibody, and by electron microscopy, using native or fluorescein-conjugated Con A and ferritin-conjugated anti-mouse Ig antibody. Prior incubation of the cells with Con A inhibited only partially capping os surface Ig, whereas it blocked almost completely capping of theta antigens. Both on cells with rings and on cells with caps the staining for surface Ig or theta antigen was superimposed to the staining for Con A. When Con A receptors on spleen cells were capped by Con A at concentrations of 10 mug/ml or higher, and the distribution of surface Ig was examined under noncapping conditions, all detectable surface Ig were found in the caps. As shown by electron microscopy, surface Ig remained dispersed in a layer of Con A. The ability of Con A to cap surface Ig was not altered by the presence of cohchicine or vinblastine. These results suggest that surface Ig are cross-linked by Con A to other Con A receptors. In these conditions surface Ig behave essentially as Con A receptors, as for example, in their sensitivity to cytochalasin B during inhibition or reversal of capping induced by this drug. The behavior of surface Ig parallels that of Con A receptors also in the presence of vinblastine. It is concluded that in the presence of Con A, antimitotic drugs do not modify directly the interaction between Con A receptors and surface Ig, but probably influence the capping ability of the Con A receptors or, more in general, affect the ability to elicit movements over the cell surface. The role in capping of cytochalasin-sensitive and vinblastine-sensitive structures is discussed. Both types of structures appear to play an active role in the formation of a cap, although the former probably corresponds to the main mechanical system responsible for the active displacement of cytoplasmic and surface material."}
{"id": "PMID:1092700", "title": "Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid. I. Light microscope observations.", "content": "Cytochalasin B (CB) induces a biphasic retraction is some cell types. The rapid response that peaks in 30 min leads to the \"dendritic\" condition. Replicating myogenic and fibrogenic cells, as well as postmitotic myoblasts and myotubes, participate in this reaction. This is followed by a slower phase that requires 40 h for stabilization and leads to the fully \"absorized\" state. Only replicating myogenic and fibrogenic cells participate in this reaction. Postmitotic myoblasts and myotubes do not arborize but round up and float off into the medium. Pretreatment with Colcemid does not block the rapid response to CB, but does block arborization. CB-arborized cells exposed to Colcemid while in the presence of CB develop sufficient tension to pull themselves apart. If CB depolymerizes actin-like filaments, and if such filaments constitute the only contractile system in the cell, then it is difficult to visualize how cells in CB develop such tension. Colcemid induces twisting, birefringent bands in interphase- and metaphase-arrested myogenic and fibrogenic cells, and in postmitotic myotubes. Such bands are more evident when CB-arborized cells are removed from CB and allowed to relax in Colcemid. These birefringent bands assemble in the prescence of cycloheximide, and may constitute 20% of the volume of the cell.", "contents": "Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid. I. Light microscope observations. Cytochalasin B (CB) induces a biphasic retraction is some cell types. The rapid response that peaks in 30 min leads to the \"dendritic\" condition. Replicating myogenic and fibrogenic cells, as well as postmitotic myoblasts and myotubes, participate in this reaction. This is followed by a slower phase that requires 40 h for stabilization and leads to the fully \"absorized\" state. Only replicating myogenic and fibrogenic cells participate in this reaction. Postmitotic myoblasts and myotubes do not arborize but round up and float off into the medium. Pretreatment with Colcemid does not block the rapid response to CB, but does block arborization. CB-arborized cells exposed to Colcemid while in the presence of CB develop sufficient tension to pull themselves apart. If CB depolymerizes actin-like filaments, and if such filaments constitute the only contractile system in the cell, then it is difficult to visualize how cells in CB develop such tension. Colcemid induces twisting, birefringent bands in interphase- and metaphase-arrested myogenic and fibrogenic cells, and in postmitotic myotubes. Such bands are more evident when CB-arborized cells are removed from CB and allowed to relax in Colcemid. These birefringent bands assemble in the prescence of cycloheximide, and may constitute 20% of the volume of the cell."}
{"id": "PMID:1092701", "title": "Nucleolar necklaces in chick embryo fibroblast cells. II. Microscope observations of the effect of adenosine analogues on nucleolar necklace formation.", "content": "The round nucleoli of chick embryo fibroblast cells, when exposed to adenosine (2 mM)or to a number of adenosine analogues, lose material and unravel over a period of several hours to become beaded strands, 20 mu M in length, termed nucleolar necklaces (NN). Light microscope observations on this process are described. Biochemical experiments have revealed that most of these analogues interfere with both messenger RNA synthesis and ribosome synthesis, causing extensive degradation of the preribosome species containing 32S RNA although most of the preribosomes containing 18S RNA survive. We suggest that it is the depletion from the nucleolus of the adhesive 32S and 28S RNA preribosomes which allows the remaining nucleolar apparatus to spread apart into the NN configuration. Also required for the maintenance of the NN structure is the synthesis of some ribosomal RNA (rRNA) possibly present as rRNA \"feathers\" on the DNA. The addition of inhibitors of rRNA synthesis such as actinomycin D to the NN-containing cells causes loss of rRNA. Then a contraction and collapse of the NN structure into small dense spheres is observed.", "contents": "Nucleolar necklaces in chick embryo fibroblast cells. II. Microscope observations of the effect of adenosine analogues on nucleolar necklace formation. The round nucleoli of chick embryo fibroblast cells, when exposed to adenosine (2 mM)or to a number of adenosine analogues, lose material and unravel over a period of several hours to become beaded strands, 20 mu M in length, termed nucleolar necklaces (NN). Light microscope observations on this process are described. Biochemical experiments have revealed that most of these analogues interfere with both messenger RNA synthesis and ribosome synthesis, causing extensive degradation of the preribosome species containing 32S RNA although most of the preribosomes containing 18S RNA survive. We suggest that it is the depletion from the nucleolus of the adhesive 32S and 28S RNA preribosomes which allows the remaining nucleolar apparatus to spread apart into the NN configuration. Also required for the maintenance of the NN structure is the synthesis of some ribosomal RNA (rRNA) possibly present as rRNA \"feathers\" on the DNA. The addition of inhibitors of rRNA synthesis such as actinomycin D to the NN-containing cells causes loss of rRNA. Then a contraction and collapse of the NN structure into small dense spheres is observed."}
{"id": "PMID:1092702", "title": "Studies of intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes (PMNS). I. Role of contact inhibition of locomotion.", "content": "Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of its collision with another cell. One hypothesis to account for intercellular invasion proposes that a necessary condition for a cell type to be invasive to a given host tissue is that it lack contact paralysis of locomotion during collision with cells of that host tissue. The hypothesis has been tested using rabbit peritoneal neutrophil granulocytes (PMNs) as the invasive cell type and chick embryo fibroblasts as the host tissue. In organ culture, PMNs rapidly invade aggregates of fibroblasts. The behavior of the pseudopods of PMNs during collision with fibroblasts was analyzed for contact paralysis by a study of time-lapse films of cells in mixed monolayer culture. In monolayer culture, PMNs show little sign of paralysis of the pseudopods upon collision with fibroblasts and thus conform in their behavior to that predicted by the hypothesis.", "contents": "Studies of intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes (PMNS). I. Role of contact inhibition of locomotion. Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of its collision with another cell. One hypothesis to account for intercellular invasion proposes that a necessary condition for a cell type to be invasive to a given host tissue is that it lack contact paralysis of locomotion during collision with cells of that host tissue. The hypothesis has been tested using rabbit peritoneal neutrophil granulocytes (PMNs) as the invasive cell type and chick embryo fibroblasts as the host tissue. In organ culture, PMNs rapidly invade aggregates of fibroblasts. The behavior of the pseudopods of PMNs during collision with fibroblasts was analyzed for contact paralysis by a study of time-lapse films of cells in mixed monolayer culture. In monolayer culture, PMNs show little sign of paralysis of the pseudopods upon collision with fibroblasts and thus conform in their behavior to that predicted by the hypothesis."}
{"id": "PMID:1092704", "title": "Electron-microscopic mapping of AT-rich regions and of E. coli RNA polymerase-binding sites on the circular kinetoplast DNA of Trypanosoma cruzi.", "content": "Partial alkaline denaturation of the circular kinetoplast DNA (kDNA) of Trypanosoma cruzi has shown the existence of 4 small, well-defined AT-rich regions with an average size of about 200 base pairs. They are almost equally distributed, separated by approximately 90 degrees on the circular molecule. All minicircles, whether free or linked in networks, have the same denaturation pattern and, therefore, seem to contain the same information. The long linear molecules present in low amounts in the kDNA samples do not show the same denaturation pattern. Partial denaturation of molecules in larger associations indicates that the circular units may be linked to each other by one strand only. kDNA can be transcribed in vitro by the RNA polymerase of E. coli. RNA polymerase-kDNA complexes have been studied in the electron microscope. By spreading the DNA-protein complexes by adhesion to positively charged carbon films and dark-field observation, it was possible to show the existence of 4 specific binding sites of the E. coli RNA polymerase on the kDNA circles. Comparing the position of the polymerase-binding sites and the AT-rich melted zones, it is suggested that a correlation exists between the two. As had been shown in earlier work, the replication of circular kDNA can be blocked by treating the trypanosomes with the trypanocidal drug Berenil. The comparison of the relative position of the Berenil-blocked replication forks with the position of the 4 denaturation loops shows that the DNA replication is stopped at these AT-rich regions. Since there is evidence that Berenil binds preferentially to AT-rich DNA and seems to be involved in inhibition of DNA replication, the following hypothetical model can be proposed. The replication of the circular kDNA molecules is discontinuous and involves the synthesis of RNA primers; when Berenil is bound to the AT-rich regions, synthesis of new RNA primers is inhibited and replication is blocked at these points, leading to the accumulation of replicating intermediates with defined branch lengths.", "contents": "Electron-microscopic mapping of AT-rich regions and of E. coli RNA polymerase-binding sites on the circular kinetoplast DNA of Trypanosoma cruzi. Partial alkaline denaturation of the circular kinetoplast DNA (kDNA) of Trypanosoma cruzi has shown the existence of 4 small, well-defined AT-rich regions with an average size of about 200 base pairs. They are almost equally distributed, separated by approximately 90 degrees on the circular molecule. All minicircles, whether free or linked in networks, have the same denaturation pattern and, therefore, seem to contain the same information. The long linear molecules present in low amounts in the kDNA samples do not show the same denaturation pattern. Partial denaturation of molecules in larger associations indicates that the circular units may be linked to each other by one strand only. kDNA can be transcribed in vitro by the RNA polymerase of E. coli. RNA polymerase-kDNA complexes have been studied in the electron microscope. By spreading the DNA-protein complexes by adhesion to positively charged carbon films and dark-field observation, it was possible to show the existence of 4 specific binding sites of the E. coli RNA polymerase on the kDNA circles. Comparing the position of the polymerase-binding sites and the AT-rich melted zones, it is suggested that a correlation exists between the two. As had been shown in earlier work, the replication of circular kDNA can be blocked by treating the trypanosomes with the trypanocidal drug Berenil. The comparison of the relative position of the Berenil-blocked replication forks with the position of the 4 denaturation loops shows that the DNA replication is stopped at these AT-rich regions. Since there is evidence that Berenil binds preferentially to AT-rich DNA and seems to be involved in inhibition of DNA replication, the following hypothetical model can be proposed. The replication of the circular kDNA molecules is discontinuous and involves the synthesis of RNA primers; when Berenil is bound to the AT-rich regions, synthesis of new RNA primers is inhibited and replication is blocked at these points, leading to the accumulation of replicating intermediates with defined branch lengths."}
{"id": "PMID:1092705", "title": "[Splenic emergencies and pregnancy].", "content": "The authors report a personal case of spontaneous rupture of the splenic vein during pregnancy, already published elsewhere [6], and study again spontaneous rupture of the splenic vessels, artery or vein, and rupture of the spleen in relation to pregnancy, either during or after labour.  In all, 150 cases were found in the world literature.  This is an extremely dangerous surgical emergency, and the mother's life is in danger immediately.  We attempted to determine the clinical and paraclinical factors which would permit one to lay down the early indications for operation in these two emergencies.  Splenectomy remains, in all cases, the basis of surgical treatment.  In cases of maternal survival, the late, general and obstetric prognosis remains good.", "contents": "[Splenic emergencies and pregnancy]. The authors report a personal case of spontaneous rupture of the splenic vein during pregnancy, already published elsewhere [6], and study again spontaneous rupture of the splenic vessels, artery or vein, and rupture of the spleen in relation to pregnancy, either during or after labour.  In all, 150 cases were found in the world literature.  This is an extremely dangerous surgical emergency, and the mother's life is in danger immediately.  We attempted to determine the clinical and paraclinical factors which would permit one to lay down the early indications for operation in these two emergencies.  Splenectomy remains, in all cases, the basis of surgical treatment.  In cases of maternal survival, the late, general and obstetric prognosis remains good."}
{"id": "PMID:1092707", "title": "Inhibitory action of somatostatin on pancreatic alpha and beta cell function.", "content": "In normal men, the administration of somatostatin almost completely abolished the glucose stimulated insulin-release seen during control studies (without somatostatin), and caused a further reduction in glucagon secretion beyond that induced by hyperglycemia. Following the infusion, there was a rapid and marked rebound for insulin but not glucagon secretion. These events were attended by a marked retardation of the glucose disappearance rate (K) which exhibited two clearly separable components; the initial slow component (mean K equals 0.64) coincided with the period of insulin suppression and was followed by a faster component (mean K equals 1.37) temporally related to the marked rebound increase in insulin release after discontinuation of the somatostatin infusion. Similarly, the addition of somatostatin infusion completely blocked the release of insulin and growth hormone and delayed the release of glucagon stimulated by arginine infusion. Following the somatostatin infusion there was a small rise in GH and a marked rebound for insulin and this was associated with a higher level of plasma glucose than that found following arginine infusion alone. These data establish that the administration of somatostatin can effectively block the release of insulin stimulated by arginine and glucose, can attenuate the release of glucagon induced by arginine and can enhance the glucose-mediated glucagon suppression. The attendance of a relative hyperglycemia during these events is probably the net result of an impediment in peripheral glucose disposition due to acute insulin lack and a decreased hepatic glucose output secondary to glucagon suppression.", "contents": "Inhibitory action of somatostatin on pancreatic alpha and beta cell function. In normal men, the administration of somatostatin almost completely abolished the glucose stimulated insulin-release seen during control studies (without somatostatin), and caused a further reduction in glucagon secretion beyond that induced by hyperglycemia. Following the infusion, there was a rapid and marked rebound for insulin but not glucagon secretion. These events were attended by a marked retardation of the glucose disappearance rate (K) which exhibited two clearly separable components; the initial slow component (mean K equals 0.64) coincided with the period of insulin suppression and was followed by a faster component (mean K equals 1.37) temporally related to the marked rebound increase in insulin release after discontinuation of the somatostatin infusion. Similarly, the addition of somatostatin infusion completely blocked the release of insulin and growth hormone and delayed the release of glucagon stimulated by arginine infusion. Following the somatostatin infusion there was a small rise in GH and a marked rebound for insulin and this was associated with a higher level of plasma glucose than that found following arginine infusion alone. These data establish that the administration of somatostatin can effectively block the release of insulin stimulated by arginine and glucose, can attenuate the release of glucagon induced by arginine and can enhance the glucose-mediated glucagon suppression. The attendance of a relative hyperglycemia during these events is probably the net result of an impediment in peripheral glucose disposition due to acute insulin lack and a decreased hepatic glucose output secondary to glucagon suppression."}
{"id": "PMID:1092708", "title": "The significance of weight loss in the evaluation of pituitary response to LH-RH in women with secondary amenorrhea.", "content": "Sixteen women with amenorrhea occurring in the setting of severe self-imposed weight loss and 18 women with secondary amenorrhea due to other causes were given LH-RH (luteinizing hormone-releasing hormone). Women with weight loss were found to be unresponsive to LH-RH when severely underweight. FSH responsiveness returned in a linear fashion as weight gain occurred and was not related to estrogen levels. LH responsiveness also returned with weight gain although the relationship was not linear but exponential and a sudden increase in responsiveness occurred at 15% below ideal weight. No relationship to estrogen levels could be found. Women who experienced amenorrhea in a setting other than weight loss did not demonstrate responsiveness to LH-RH which could be correlated with body mass, even when underweight. Women who experienced amenorrhea with weight loss had a consistently lower LH response to LH-RH than the second group and their LH response was always lower than the FSH response. On the other hand, a variety of patterns was found in women with amenorrhea due to other causes.", "contents": "The significance of weight loss in the evaluation of pituitary response to LH-RH in women with secondary amenorrhea. Sixteen women with amenorrhea occurring in the setting of severe self-imposed weight loss and 18 women with secondary amenorrhea due to other causes were given LH-RH (luteinizing hormone-releasing hormone). Women with weight loss were found to be unresponsive to LH-RH when severely underweight. FSH responsiveness returned in a linear fashion as weight gain occurred and was not related to estrogen levels. LH responsiveness also returned with weight gain although the relationship was not linear but exponential and a sudden increase in responsiveness occurred at 15% below ideal weight. No relationship to estrogen levels could be found. Women who experienced amenorrhea in a setting other than weight loss did not demonstrate responsiveness to LH-RH which could be correlated with body mass, even when underweight. Women who experienced amenorrhea with weight loss had a consistently lower LH response to LH-RH than the second group and their LH response was always lower than the FSH response. On the other hand, a variety of patterns was found in women with amenorrhea due to other causes."}
{"id": "PMID:1092709", "title": "Inhibition of luteinizing hormone secretion induced by synthetic LRH by long-term treatment with glucocorticoids in human subjects.", "content": "The response of luteinizing hormone to a single injection of synthetic LRH was established in 6 women and 7 men following an intramuscular dose of 0.2mg or 0.1mg. Significant increases  of serum LH were observed in 30 min. Pretreatment with metyrapone for one day (4.5 g/sugject injection of synthetic LRH (0.1 MG/SUBJECT). Seven women, 21-48 years of age who were treated with prednisolone for a least 11/2 months were examined for responsiveness of the anterior pituitary to a single injection of synthetic LRH (0.2 MG). The secretion of LH was markedly suppressed and maximal serum levels of LH WERE OBSERVED 90 MIN FOLLOWING LRH injection.", "contents": "Inhibition of luteinizing hormone secretion induced by synthetic LRH by long-term treatment with glucocorticoids in human subjects. The response of luteinizing hormone to a single injection of synthetic LRH was established in 6 women and 7 men following an intramuscular dose of 0.2mg or 0.1mg. Significant increases  of serum LH were observed in 30 min. Pretreatment with metyrapone for one day (4.5 g/sugject injection of synthetic LRH (0.1 MG/SUBJECT). Seven women, 21-48 years of age who were treated with prednisolone for a least 11/2 months were examined for responsiveness of the anterior pituitary to a single injection of synthetic LRH (0.2 MG). The secretion of LH was markedly suppressed and maximal serum levels of LH WERE OBSERVED 90 MIN FOLLOWING LRH injection."}
{"id": "PMID:1092710", "title": "Isolated deficiency of follicle-stimulating hormone: Further studies.", "content": "We record further studies over the past 2 yr on a unique female subject with isolated follicle-stimulating hormone (hFSH) deficiency, who developed human anti-gFSH antibodies after treatment with exogenous urinary gonadotropins. Administration of LRH resulted in a significant rise in serum hLH, but hFSH levels remained undetectable, \"alpha Subunit\" (the common alpha chain of the glycoprotein hormones) was detectable in basal samples obtained from our patient, and rose sharply after LRH. This is concordant with the hypothesis that the defect in our subject may be in the synthesis of the beta chain of hFSH, but it does not exclude other possibilities. The concentration of hFSH antibodies in the patient's serum has been monitored and her response to a further course of exogenous gonadotropins is recorded. The anti serum exhibits specificity for the hFSH molecule; the alpha and the beta chains of hFSH are virtually inert in competing with tracer 125-I-hFSH for binding to the antibody.", "contents": "Isolated deficiency of follicle-stimulating hormone: Further studies. We record further studies over the past 2 yr on a unique female subject with isolated follicle-stimulating hormone (hFSH) deficiency, who developed human anti-gFSH antibodies after treatment with exogenous urinary gonadotropins. Administration of LRH resulted in a significant rise in serum hLH, but hFSH levels remained undetectable, \"alpha Subunit\" (the common alpha chain of the glycoprotein hormones) was detectable in basal samples obtained from our patient, and rose sharply after LRH. This is concordant with the hypothesis that the defect in our subject may be in the synthesis of the beta chain of hFSH, but it does not exclude other possibilities. The concentration of hFSH antibodies in the patient's serum has been monitored and her response to a further course of exogenous gonadotropins is recorded. The anti serum exhibits specificity for the hFSH molecule; the alpha and the beta chains of hFSH are virtually inert in competing with tracer 125-I-hFSH for binding to the antibody."}
{"id": "PMID:1092711", "title": "The diagnosis of Hashimoto's thyroiditis.", "content": "Computer methods were used to estimate the usefulness of several clinical signs and laboratory tests in the diagnosis of chronic lymphocytic (Hashimoto's) thyroiditis. Information was drawn from the records of 217 patients with this disease seen at two hospitals in Southern California. The parameters studied included the physical characteristics of the thyroid gland; the appearance of the radioisotope thyroid scan; the response to a perchlorate discharge test; the serum antithyroglobulin antibody titer; the serum TSH concentration measured by radioimmunoassay; the thyroid radioiodine uptake response to exogenous TSH stimulation; and the serum PBI-T4I difference. Of the above, the PBI-T4I difference was deleted, being of limited value. The TSH stimulation test and serum TSH measurement were considered as alternative ways to evaluate thyroid reserve. Therefore, five diagnostic markers remained, all useful but not definitive. Computer and rule-of-thumb methods (two or more of the five markers positive) were tested for successful diagnosis of 145 patients with Hashimoto's thyroiditis proven by pathological examination of biopsied tissue; 23 of these were patients not used in the original pool of data. As a rule of thumb, it was found that Hashimoto's thyroiditis is the likely diagnosis if two or more out of the five useful markers are in its favor. For best results, four and preferably all of the five criteria should be tested. If this is done, the expected diagnostic accuracy for patients with Hashimoto's disease is by the rule-of-thumb method 67% correct, 21% indecisive, and 12% false negative; by computer methods it is 88% correct, 4% indecisive, and 8% false negative. By each method the number of false positive diagnoses was equivalent to 25% of the total number of patients with this disease. The false positive results nearly all occurred in patients with goiter associated with a defect in thyroid hormone synthesis.", "contents": "The diagnosis of Hashimoto's thyroiditis. Computer methods were used to estimate the usefulness of several clinical signs and laboratory tests in the diagnosis of chronic lymphocytic (Hashimoto's) thyroiditis. Information was drawn from the records of 217 patients with this disease seen at two hospitals in Southern California. The parameters studied included the physical characteristics of the thyroid gland; the appearance of the radioisotope thyroid scan; the response to a perchlorate discharge test; the serum antithyroglobulin antibody titer; the serum TSH concentration measured by radioimmunoassay; the thyroid radioiodine uptake response to exogenous TSH stimulation; and the serum PBI-T4I difference. Of the above, the PBI-T4I difference was deleted, being of limited value. The TSH stimulation test and serum TSH measurement were considered as alternative ways to evaluate thyroid reserve. Therefore, five diagnostic markers remained, all useful but not definitive. Computer and rule-of-thumb methods (two or more of the five markers positive) were tested for successful diagnosis of 145 patients with Hashimoto's thyroiditis proven by pathological examination of biopsied tissue; 23 of these were patients not used in the original pool of data. As a rule of thumb, it was found that Hashimoto's thyroiditis is the likely diagnosis if two or more out of the five useful markers are in its favor. For best results, four and preferably all of the five criteria should be tested. If this is done, the expected diagnostic accuracy for patients with Hashimoto's disease is by the rule-of-thumb method 67% correct, 21% indecisive, and 12% false negative; by computer methods it is 88% correct, 4% indecisive, and 8% false negative. By each method the number of false positive diagnoses was equivalent to 25% of the total number of patients with this disease. The false positive results nearly all occurred in patients with goiter associated with a defect in thyroid hormone synthesis."}
{"id": "PMID:1092712", "title": "Effect of synthetic luteinizing hormone releasing hormone (LH-RH) on the release of gonadotropins in Cushing's disease.", "content": "The effect of intravenous synthetic luteinizing hormone releasing hormone (LH-RH) on plasma radioimmunoassayable levels of gonadotropins was investigated in 6 women suffering from Cushing's disease with bilateral adrenal hyperplasia. In five of six cases no significant variation of plasma LH levels was found following stimulation; in one case the response to LH-RH was present although slightly reduced below the normal range. By contrast in all cases the plasma FSH response was similar to that recorded in normal subjects. The explanation of impaired LH response is not clear but the possibility that endogenous hypercortisolism affects the pituitary responsiveness to LH-RH has to be considered.", "contents": "Effect of synthetic luteinizing hormone releasing hormone (LH-RH) on the release of gonadotropins in Cushing's disease. The effect of intravenous synthetic luteinizing hormone releasing hormone (LH-RH) on plasma radioimmunoassayable levels of gonadotropins was investigated in 6 women suffering from Cushing's disease with bilateral adrenal hyperplasia. In five of six cases no significant variation of plasma LH levels was found following stimulation; in one case the response to LH-RH was present although slightly reduced below the normal range. By contrast in all cases the plasma FSH response was similar to that recorded in normal subjects. The explanation of impaired LH response is not clear but the possibility that endogenous hypercortisolism affects the pituitary responsiveness to LH-RH has to be considered."}
{"id": "PMID:1092713", "title": "Abnormal pancreatic alpha-cell function in first-degree relatives of known diabetics.", "content": "A standard intravenous glucose tolerance test utilizing a 25g glucose load was administered to 15 first-degree relatives of known diabetics and 19 normal control subjects with no known diabetic relatives. Despite normal glucose tolerance, 5 of the first-degree relatives had a significantly lower release of first-phase insulin (p 0.05). In contrast to 8 normal subjects and the remaining 10 first-degree relatives the secretion of glucagon by the 5 first degree relatives with abnormally low insulin release was not suppressed by induced hyperglycaemia. These findings provide evidence of abnormal alpha-cell function in a very early stage of genetic diabetes.", "contents": "Abnormal pancreatic alpha-cell function in first-degree relatives of known diabetics. A standard intravenous glucose tolerance test utilizing a 25g glucose load was administered to 15 first-degree relatives of known diabetics and 19 normal control subjects with no known diabetic relatives. Despite normal glucose tolerance, 5 of the first-degree relatives had a significantly lower release of first-phase insulin (p 0.05). In contrast to 8 normal subjects and the remaining 10 first-degree relatives the secretion of glucagon by the 5 first degree relatives with abnormally low insulin release was not suppressed by induced hyperglycaemia. These findings provide evidence of abnormal alpha-cell function in a very early stage of genetic diabetes."}
{"id": "PMID:1092714", "title": "Studies with human leukocyte lysosomes. Evidence for antilysosome antibodies in lupus erythematosus and for the presence of lysosomal antigen in inflammatory diseases.", "content": "Human lysosomes were isolated from normal peripheral blood leukoyctes and characterized by electron microscopy, enzyme analysis, and assays for DNA and RNA. Stored sera from 37 unselected patients with systemic lupus erythematosus (SLE), including active and inactive, treated and untreated cases, were tested in complement fixation (CF) reactions with these lysosome preparations. 23 SLE sera exhibited positive CR reactions, as did sera from two patients with \"lupoid\" hepatitis. The seven SLE sera with strongest CF reactivity also demonstrated gel precipitin reactions with lysosomes. Neither CF nor precipitin reactions with lysosomes were observed with normal sera or with sera of patients with drug-induced lupus syndrome, rheumatoid arthritis (RA), polymyositis, or autoimmune hemolytic anemia. By several criteria the antilysosome CF and precipitin reactions of SLE sera cound not be attributed to antibody to DNA, RNA, or other intracellular organelles. The lysosomal component reactive with SLE sera in CF assays was sedimentable at high speed and is presumably membrane associated. The CF activity of two representative SLE sera was associated with IgG globulins by Sephadex filtration. A search for lysosomal antigen in SLE and related disorders was also made. By employing rabbit antiserum to human lysosomes in immunodiffusion, a soluble lysosomal component, apparently distinct from the sedimentable (membrane-associated) antigen described above, was identified in serum, synovial fluid, or pleural fluid from patients with SLE, RA, ankylosing spondylitis, and leukemoid reaction. An antigenically identical soluble component reactive with the rabbit antiserum could be released in vitro from intact lysosomes by repeated freeze-thaw cycles..", "contents": "Studies with human leukocyte lysosomes. Evidence for antilysosome antibodies in lupus erythematosus and for the presence of lysosomal antigen in inflammatory diseases. Human lysosomes were isolated from normal peripheral blood leukoyctes and characterized by electron microscopy, enzyme analysis, and assays for DNA and RNA. Stored sera from 37 unselected patients with systemic lupus erythematosus (SLE), including active and inactive, treated and untreated cases, were tested in complement fixation (CF) reactions with these lysosome preparations. 23 SLE sera exhibited positive CR reactions, as did sera from two patients with \"lupoid\" hepatitis. The seven SLE sera with strongest CF reactivity also demonstrated gel precipitin reactions with lysosomes. Neither CF nor precipitin reactions with lysosomes were observed with normal sera or with sera of patients with drug-induced lupus syndrome, rheumatoid arthritis (RA), polymyositis, or autoimmune hemolytic anemia. By several criteria the antilysosome CF and precipitin reactions of SLE sera cound not be attributed to antibody to DNA, RNA, or other intracellular organelles. The lysosomal component reactive with SLE sera in CF assays was sedimentable at high speed and is presumably membrane associated. The CF activity of two representative SLE sera was associated with IgG globulins by Sephadex filtration. A search for lysosomal antigen in SLE and related disorders was also made. By employing rabbit antiserum to human lysosomes in immunodiffusion, a soluble lysosomal component, apparently distinct from the sedimentable (membrane-associated) antigen described above, was identified in serum, synovial fluid, or pleural fluid from patients with SLE, RA, ankylosing spondylitis, and leukemoid reaction. An antigenically identical soluble component reactive with the rabbit antiserum could be released in vitro from intact lysosomes by repeated freeze-thaw cycles.."}
{"id": "PMID:1092715", "title": "Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants.", "content": "Prospective recipients of kidney transplants were tested for lymphocytotoxicity; from these we selected 102 sera that lacked cytotoxic antibodies against peripheral lymphocytes from at least 80 unrelated subjects. To detect humoral sensitization, we then reacted these with 17 cultured human lymphoid cell lines having different HL-A phenotypes. Cytotoxic antibodies reacting with these cultured cells were now detected in some of the sera. These antibodies were not directed against HL-A antigens, yet mediated lysis of target cells in the presence of rabbit but not of human or guinea pig complement. Furthermore, they activated the classical pathway of the rabbit complement system. Later, a significant association was found between occurrence of cytotoxic antibodies and rejection of the transplant. Thus, cultured human lymphoid cells, because of their great susceptibility to complement-mediated lysis, appear to be useful in detecting humoral sensitization in candidates for kidney grafts.", "contents": "Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants. Prospective recipients of kidney transplants were tested for lymphocytotoxicity; from these we selected 102 sera that lacked cytotoxic antibodies against peripheral lymphocytes from at least 80 unrelated subjects. To detect humoral sensitization, we then reacted these with 17 cultured human lymphoid cell lines having different HL-A phenotypes. Cytotoxic antibodies reacting with these cultured cells were now detected in some of the sera. These antibodies were not directed against HL-A antigens, yet mediated lysis of target cells in the presence of rabbit but not of human or guinea pig complement. Furthermore, they activated the classical pathway of the rabbit complement system. Later, a significant association was found between occurrence of cytotoxic antibodies and rejection of the transplant. Thus, cultured human lymphoid cells, because of their great susceptibility to complement-mediated lysis, appear to be useful in detecting humoral sensitization in candidates for kidney grafts."}
{"id": "PMID:1092716", "title": "Immunopathological changes in rheumatoid arthritis and other joint diseases.", "content": "A comparative study of the distribution of immunoglobulins G, M, and A and C3 in the synovium and inside synovial fluid leucocytes and of the relative levels of IgG, IgM, AND C3 in paired samples of serum and synovial fluid from both seropositive and seronegative patients with rheumatoid arthritis and other types of non-infective synovitis shows that although there is no distinctive immunopathological feature of rheumatoid arthritis, the incidence of immune complexes containing IgG and IgM with and without detectable C3 in the affected synovium or inside synovial fluid granulocytes is higher in rheumatoid arthritis and especially so in seropositive cases. The mean level of C3 in synovial fluid from patients with rheumatoid arthritis is lower than that from the group without rheumatoid arthritis. In contrast to previous reports, extracellular clumps of IgA could be detected in the affected synovium of a number of affected patients. Aggretated human IgG could be bound by some of the synovial biopsies and synovial fluid leucocytes from both seropositive and seronegative rheumatoid arthritis patients. Antinuclear factor and rheumatoid factor could be detected in the synovial fluid but not in the serum of several patients suggesting either selective sequestration or local synthesis of antinuclear and rheumatoid factors in the affected joints.", "contents": "Immunopathological changes in rheumatoid arthritis and other joint diseases. A comparative study of the distribution of immunoglobulins G, M, and A and C3 in the synovium and inside synovial fluid leucocytes and of the relative levels of IgG, IgM, AND C3 in paired samples of serum and synovial fluid from both seropositive and seronegative patients with rheumatoid arthritis and other types of non-infective synovitis shows that although there is no distinctive immunopathological feature of rheumatoid arthritis, the incidence of immune complexes containing IgG and IgM with and without detectable C3 in the affected synovium or inside synovial fluid granulocytes is higher in rheumatoid arthritis and especially so in seropositive cases. The mean level of C3 in synovial fluid from patients with rheumatoid arthritis is lower than that from the group without rheumatoid arthritis. In contrast to previous reports, extracellular clumps of IgA could be detected in the affected synovium of a number of affected patients. Aggretated human IgG could be bound by some of the synovial biopsies and synovial fluid leucocytes from both seropositive and seronegative rheumatoid arthritis patients. Antinuclear factor and rheumatoid factor could be detected in the synovial fluid but not in the serum of several patients suggesting either selective sequestration or local synthesis of antinuclear and rheumatoid factors in the affected joints."}
{"id": "PMID:1092717", "title": "The distribution of muramidase (lysozyme) in human tissues.", "content": "The distribution of muramidase (lysozyme) in normal and pathological human tissues has been studied, using an immunohistological technique. The enzyme was demonstrated in a variety of healthy tissues, including serous salivary acinar cells, lactating mammary tissue, Paneth cells, renal tubular cells, myeloid cells (including eosinophils), and histiocytic cells. In pathological tissues the most striking positivity was encountered in reactive histiocytic cells in granulomatous conditions such as tuberculosis and Crohn's disease. The finding of this study are related to previous reports of the distribution of human and animal muramidase and the implications of patterns of muramidase staining in pathological histiocytes are briefly discussed.", "contents": "The distribution of muramidase (lysozyme) in human tissues. The distribution of muramidase (lysozyme) in normal and pathological human tissues has been studied, using an immunohistological technique. The enzyme was demonstrated in a variety of healthy tissues, including serous salivary acinar cells, lactating mammary tissue, Paneth cells, renal tubular cells, myeloid cells (including eosinophils), and histiocytic cells. In pathological tissues the most striking positivity was encountered in reactive histiocytic cells in granulomatous conditions such as tuberculosis and Crohn's disease. The finding of this study are related to previous reports of the distribution of human and animal muramidase and the implications of patterns of muramidase staining in pathological histiocytes are briefly discussed."}
{"id": "PMID:1092718", "title": "Isolations of aerobic sporing bacilli from the tips of indwelling intravascular catheters.", "content": "Following previous reports of the isolation of aerobic sporing bacilli from intravenous catheters and infusion sets, it was decided to analyse a series of catheter tip cultures to ascertain the significance of these isolations. Those catheters from which aerobic sporing bacilli were isolated behaved statistically differently from other catheters from which organisms were isolated, but were statistically similar to sterile catheters. Using the nitroblue tetrazolium (NBT) test no significant difference was found between patients with isolates of aerobic sporing bacilli and those with sterile catheters. The NBT test readily distinguished those patients from whom other organisms were isolated. In a small number of instances some evidence of a possible pathogenic role for the aerobic sporing bacillus was found, but the results clearly indicate that in most cases these organisms can be regarded as contaminants.", "contents": "Isolations of aerobic sporing bacilli from the tips of indwelling intravascular catheters. Following previous reports of the isolation of aerobic sporing bacilli from intravenous catheters and infusion sets, it was decided to analyse a series of catheter tip cultures to ascertain the significance of these isolations. Those catheters from which aerobic sporing bacilli were isolated behaved statistically differently from other catheters from which organisms were isolated, but were statistically similar to sterile catheters. Using the nitroblue tetrazolium (NBT) test no significant difference was found between patients with isolates of aerobic sporing bacilli and those with sterile catheters. The NBT test readily distinguished those patients from whom other organisms were isolated. In a small number of instances some evidence of a possible pathogenic role for the aerobic sporing bacillus was found, but the results clearly indicate that in most cases these organisms can be regarded as contaminants."}
{"id": "PMID:1092719", "title": "Observations on the accuracy of point counting including a description of a new graticule.", "content": "The accuracy of point counting as a histometric technique depends very much on the number of points counted, and the number necessary varies greatly with both the level of accuracy required and the proportion the relevant tissue occupies in the section. It is shown that the number of \"hits\" is a more useful measure than the total number of points counted, and a simple rule derived for determining the number of hits necessary to achieve any necessary degree of accuracy. A method of progressive estimation of accuracy when attempting to measure the proportion of a tissue that is unevenly distributed is also described. The point-counting graticules commonly used, with 25 points, are inadequate with tissue proportions below 10%. A new graticule design, with 100 points optimally distributed, and usable as a 25-point array if required, is illustrated.", "contents": "Observations on the accuracy of point counting including a description of a new graticule. The accuracy of point counting as a histometric technique depends very much on the number of points counted, and the number necessary varies greatly with both the level of accuracy required and the proportion the relevant tissue occupies in the section. It is shown that the number of \"hits\" is a more useful measure than the total number of points counted, and a simple rule derived for determining the number of hits necessary to achieve any necessary degree of accuracy. A method of progressive estimation of accuracy when attempting to measure the proportion of a tissue that is unevenly distributed is also described. The point-counting graticules commonly used, with 25 points, are inadequate with tissue proportions below 10%. A new graticule design, with 100 points optimally distributed, and usable as a 25-point array if required, is illustrated."}
{"id": "PMID:1092720", "title": "Immunofluorescence in duodenal mucosa of children with acute enteritis due to a new virus.", "content": "Electron microscopy od duodenal mucosa from children with acute non-bacterial enteritis has shown virus particles in epithelial cells. Indirect immunofluorescent techniques applied to the same tissue showed virus antigen localized in the cytoplasm of epithelial cells of the villi. Specific IgM antibody was present in sera from infected patients as early as two days after the onset of symptoms. Virus particles from different patients appeared to share a common antigen. The evidence presented supports our belief that this new virus was the cause of acute enteritis in the children studied.", "contents": "Immunofluorescence in duodenal mucosa of children with acute enteritis due to a new virus. Electron microscopy od duodenal mucosa from children with acute non-bacterial enteritis has shown virus particles in epithelial cells. Indirect immunofluorescent techniques applied to the same tissue showed virus antigen localized in the cytoplasm of epithelial cells of the villi. Specific IgM antibody was present in sera from infected patients as early as two days after the onset of symptoms. Virus particles from different patients appeared to share a common antigen. The evidence presented supports our belief that this new virus was the cause of acute enteritis in the children studied."}
{"id": "PMID:1092721", "title": "Cell receptor studies on seven cases of diffuse histiocytic malignant lymphoma (reticulum cell sarcoma).", "content": "Expression of B and T lymphocyte receptors has been studied in seven cases of reticulum cell sarcoma. In one case, surface receptors and tests of phagocytic function demonstrated the histiocytic origin of the neoplastic cells. In four cases, tumour cells expressed both B and T lymphocyte markers (two cases) or showed a normal pattern of expression of B and T lymphocyte markers. In the other two cases, lymphocyte receptors were not detected, and there was no evidence of phagocytic function: this class of receptor-silent tumours is of uncertain pathogenesis. The significance of these observations is discussed.", "contents": "Cell receptor studies on seven cases of diffuse histiocytic malignant lymphoma (reticulum cell sarcoma). Expression of B and T lymphocyte receptors has been studied in seven cases of reticulum cell sarcoma. In one case, surface receptors and tests of phagocytic function demonstrated the histiocytic origin of the neoplastic cells. In four cases, tumour cells expressed both B and T lymphocyte markers (two cases) or showed a normal pattern of expression of B and T lymphocyte markers. In the other two cases, lymphocyte receptors were not detected, and there was no evidence of phagocytic function: this class of receptor-silent tumours is of uncertain pathogenesis. The significance of these observations is discussed."}
{"id": "PMID:1092723", "title": "Sisomicin: Bacteriological and clinical evaluation.", "content": "A preliminary study was conducted with sisomicin, a new aminoglycoside antibiotic. The drug was administered to 40 patients in doses varying from 1.5 to 3.75 mg/kg/day. Sisomicin proved to be an effective therapy in urinary tract infections and to a lesser extent in wound infections caused by gram-negative rods; favorable results have been observed in 91.6% and in 66.6% of the patients presenting these infections. Toxic reactions involving the hearing function, renal function, and general tolerance were infrequent: they occurred in less than 5% of the patients in this series. (However in approximately 22% of the patients, there was a transient appearance of granular casts in the urine.", "contents": "Sisomicin: Bacteriological and clinical evaluation. A preliminary study was conducted with sisomicin, a new aminoglycoside antibiotic. The drug was administered to 40 patients in doses varying from 1.5 to 3.75 mg/kg/day. Sisomicin proved to be an effective therapy in urinary tract infections and to a lesser extent in wound infections caused by gram-negative rods; favorable results have been observed in 91.6% and in 66.6% of the patients presenting these infections. Toxic reactions involving the hearing function, renal function, and general tolerance were infrequent: they occurred in less than 5% of the patients in this series. (However in approximately 22% of the patients, there was a transient appearance of granular casts in the urine."}
{"id": "PMID:1092724", "title": "The bioavailability of meperidine using urine assays for meperidine and normeperidine.", "content": "The results of a two-way crossover trial of two commercial preparations of meperidine hydrochloride in 24 healthy volunteers is reported. The urinary excretion patterns resulting from dosing with the two preparations were followed for 48 hours, and the urine concentrations of meperidine and a major metabolite, normeperidine, were determined by gas liquid chromatography. Statistical analysis of the data showed no significant difference between the urine concentrations of meperidine and normeperidine produced by either preparation, and the preparations are of comparable bioavailability. The use of the urinary excretion pattern and concentrations represents a rapid, accurate, and quantitative method for determining the bioavailability of different preparations of meperidine hydrochloride.", "contents": "The bioavailability of meperidine using urine assays for meperidine and normeperidine. The results of a two-way crossover trial of two commercial preparations of meperidine hydrochloride in 24 healthy volunteers is reported. The urinary excretion patterns resulting from dosing with the two preparations were followed for 48 hours, and the urine concentrations of meperidine and a major metabolite, normeperidine, were determined by gas liquid chromatography. Statistical analysis of the data showed no significant difference between the urine concentrations of meperidine and normeperidine produced by either preparation, and the preparations are of comparable bioavailability. The use of the urinary excretion pattern and concentrations represents a rapid, accurate, and quantitative method for determining the bioavailability of different preparations of meperidine hydrochloride."}
{"id": "PMID:1092725", "title": "Loxapine in newly admitted chronic schizophrenic patients.", "content": "The standard drug Stelazine (STEL), at a dose of 50 mg/day, exhibited therapeutic activity significantly different from placebo (PL) activity on several variables,  most notably BPRS, attesting to the sensitivity of the experiment. On the other hand, the investigational drug, loxapine (LOX), in doses of 100 mg/day for four weeks, could be differentiated from PL as treatment in the described population on only one variable (NGI-Imp.) and one item of the BPRS. On several variables, positive trends were noted, but the differences from PL did not attain the critical values necessary for statistical significance at P smaller than 0.05. One might speculate that the relatively short duration of treatment in this study might account for the difference between these disappointing results and the more gratifying results of a previous loxapine study in chronic long-term institutionalized schizophrenics with the same oral dose.", "contents": "Loxapine in newly admitted chronic schizophrenic patients. The standard drug Stelazine (STEL), at a dose of 50 mg/day, exhibited therapeutic activity significantly different from placebo (PL) activity on several variables,  most notably BPRS, attesting to the sensitivity of the experiment. On the other hand, the investigational drug, loxapine (LOX), in doses of 100 mg/day for four weeks, could be differentiated from PL as treatment in the described population on only one variable (NGI-Imp.) and one item of the BPRS. On several variables, positive trends were noted, but the differences from PL did not attain the critical values necessary for statistical significance at P smaller than 0.05. One might speculate that the relatively short duration of treatment in this study might account for the difference between these disappointing results and the more gratifying results of a previous loxapine study in chronic long-term institutionalized schizophrenics with the same oral dose."}
{"id": "PMID:1092726", "title": "Naproxen: long-term study in rheumatoid arthritis and \"placebo pulse\".", "content": "Naproxen is by now a relatively well-known antirheumatic drug, and many short-term studies have shown its efficacy and relatively good tolerance. We have observed 64 patients with definite or classical rheumatoid disease, 27 of whom have been followed for well over two years on daily doses of naproxen to ascertain the persistence of drug efficacy and safety. In this part of our study, patients were subjected to complete clinical and biochemical evaluations at two-monthly intervals. Naproxen was well tolerated, and the few side effects reported were transient and mild in nature. Sequential laboratory studies revealed no significant anomaly. Clinical evaluation showed no pattern suggestive of decreasing antirheumatic activity. A question frequently encountered in the treatment of certain diseases such as rheumatoid arthritis is whether long-term improvement is due to efficacious suppressive therapy or spontaneous abatement of disease activity. We devised a double-blind placebo pulse phase in which 19 of our 28 long-term patients participated in a study within a study lasting four weeks. They were divided into two groups. The first group took their usual dose of naproxen during the first two weeks and a corresponding number of placebo tablets in the next two weeks. The procedure was reversed in the other group. We conclude that naproxen remains efficacious.", "contents": "Naproxen: long-term study in rheumatoid arthritis and \"placebo pulse\". Naproxen is by now a relatively well-known antirheumatic drug, and many short-term studies have shown its efficacy and relatively good tolerance. We have observed 64 patients with definite or classical rheumatoid disease, 27 of whom have been followed for well over two years on daily doses of naproxen to ascertain the persistence of drug efficacy and safety. In this part of our study, patients were subjected to complete clinical and biochemical evaluations at two-monthly intervals. Naproxen was well tolerated, and the few side effects reported were transient and mild in nature. Sequential laboratory studies revealed no significant anomaly. Clinical evaluation showed no pattern suggestive of decreasing antirheumatic activity. A question frequently encountered in the treatment of certain diseases such as rheumatoid arthritis is whether long-term improvement is due to efficacious suppressive therapy or spontaneous abatement of disease activity. We devised a double-blind placebo pulse phase in which 19 of our 28 long-term patients participated in a study within a study lasting four weeks. They were divided into two groups. The first group took their usual dose of naproxen during the first two weeks and a corresponding number of placebo tablets in the next two weeks. The procedure was reversed in the other group. We conclude that naproxen remains efficacious."}
{"id": "PMID:1092727", "title": "Naproxen and aspirin in rheumatoid arthritis: a multicenter double-blind crossover comparison study.", "content": "One hundred nineteen adults with active definite or classical rheumatoid arthritis were studied in a multicenter double-blind crossover study of naproxen (500 mg/day) and aspirin (3.6 Gm/day). Each drug was given in sequence for a six-week study period. Patients already receiving corticosteriod and/or gold therapy were maintained at constant dose throughout the study, but analgesics and other nonsteroidal antiinflammatory agents were discontinued at baseline. Objective and subjective evaluations by both investigator and patient were carried out at two-week intervals. No significant difference in global evaluation of efficacy or individual measures of efficacy was observed between aspirin and naproxen therapy, although physicians' global evaluation tended to favor naproxen. Sedimentation rate was lower on aspirin (naproxen 43.1 mm/hr; aspirin 38.7 mm/hr; P=0.02). Naproxen, 250 mg twice daily, was significantly better tolerated than aspirin, 900 mg four times daily. Mild, moderate, and severe side effects were less frequent with naproxen. The incidence of heartburn was significantly lower on naproxen, and significantly fewer patients terminated their six-week study period on naproxen than on aspirin. There were no significant deviations from baseline values in hematocrit, white cell or differential counts, or in tests of renal and hepatic function during the course of the study.", "contents": "Naproxen and aspirin in rheumatoid arthritis: a multicenter double-blind crossover comparison study. One hundred nineteen adults with active definite or classical rheumatoid arthritis were studied in a multicenter double-blind crossover study of naproxen (500 mg/day) and aspirin (3.6 Gm/day). Each drug was given in sequence for a six-week study period. Patients already receiving corticosteriod and/or gold therapy were maintained at constant dose throughout the study, but analgesics and other nonsteroidal antiinflammatory agents were discontinued at baseline. Objective and subjective evaluations by both investigator and patient were carried out at two-week intervals. No significant difference in global evaluation of efficacy or individual measures of efficacy was observed between aspirin and naproxen therapy, although physicians' global evaluation tended to favor naproxen. Sedimentation rate was lower on aspirin (naproxen 43.1 mm/hr; aspirin 38.7 mm/hr; P=0.02). Naproxen, 250 mg twice daily, was significantly better tolerated than aspirin, 900 mg four times daily. Mild, moderate, and severe side effects were less frequent with naproxen. The incidence of heartburn was significantly lower on naproxen, and significantly fewer patients terminated their six-week study period on naproxen than on aspirin. There were no significant deviations from baseline values in hematocrit, white cell or differential counts, or in tests of renal and hepatic function during the course of the study."}
{"id": "PMID:1092728", "title": "A double-blind comparison of naproxen with indomethacin in osteoarthrosis.", "content": "Twenty-two patients with osteoarthrosis of one or both knee joints and 28 patients with osteoarthrosis of one or both hips completed a double-blind trial of 500 mg naproxen daily versus 100 mg indomethacin daily. All patients had been on other active medication up to the start of the trial. Identical trial designs were followed with both classes of patients, namely, a crossover pattern of four weeks on each drug with patients being assessed at -2, 0, 2, 4, 6, and 8 weeks. Assessments made included objective measurement of joint range, stair climbing and walking times, and subjective grading of pain present during normal activity, of which the patient kept a daily record. Patients were also questioned at each clinic visit regarding possible side effects. Study groups were comparable for both drugs. In the majority of subjective and objective parameters, there were significant improvements from baseline on both drugs of statistically comparable magnitude. Significantly fewer side effects were noted during the period on naproxen compared with those on indomethacin. There were no abnormalities discovered in hematologic or biochemical tests performed during the course of the trial.", "contents": "A double-blind comparison of naproxen with indomethacin in osteoarthrosis. Twenty-two patients with osteoarthrosis of one or both knee joints and 28 patients with osteoarthrosis of one or both hips completed a double-blind trial of 500 mg naproxen daily versus 100 mg indomethacin daily. All patients had been on other active medication up to the start of the trial. Identical trial designs were followed with both classes of patients, namely, a crossover pattern of four weeks on each drug with patients being assessed at -2, 0, 2, 4, 6, and 8 weeks. Assessments made included objective measurement of joint range, stair climbing and walking times, and subjective grading of pain present during normal activity, of which the patient kept a daily record. Patients were also questioned at each clinic visit regarding possible side effects. Study groups were comparable for both drugs. In the majority of subjective and objective parameters, there were significant improvements from baseline on both drugs of statistically comparable magnitude. Significantly fewer side effects were noted during the period on naproxen compared with those on indomethacin. There were no abnormalities discovered in hematologic or biochemical tests performed during the course of the trial."}
{"id": "PMID:1092729", "title": "Ankylosing spondylitis: open long-term and double-blind crossover studies with naproxen.", "content": "Participation in open and double-blind crossover studies in rheumatoid arthritis confirmed that naproxen improved pain and stiffness. This observation suggested that naproxen might be effective in ankylosing spondylitis. The initial trial was open, but at six months, a double-blind crossover \"placebo pulse\" was superimposed on the open trial. Thirty-six patients entered the trial taking a daily dose of 500 mg naproxen. At the end of the first month, 35 assessed naproxen as being equally effective to, or better than, previous therapy. The first ten patients to complete six months on naproxen took part in a placebo pulse study comprising two consecutive four-week periods, in one of which each patient took 500 mg naproxen, in the other, identical placebo capsules, the order being randomized. Eight patients correctly identified the placebo capsules (P=0.02). During the 16 months of trial, six patients have withdrawn, two being in remission and four for lack of efficacy. The remaining 30 patients have completed six months and 22 have completed at least 12 months on naproxen. At the end of six months pain was less (P=0.02), morning stiffness had decreased (P less than 0.01), and immobility stiffness had improved (P less than 0.01). These patients are impressed by the improvement in pain and stiffness and have little disability. All continue full-time employment and have been able to increase their leisure activities without discomfort. No persistent side effects were observed, and naproxen appears to be a useful drug in the treatment of anklyosing spondylitis.", "contents": "Ankylosing spondylitis: open long-term and double-blind crossover studies with naproxen. Participation in open and double-blind crossover studies in rheumatoid arthritis confirmed that naproxen improved pain and stiffness. This observation suggested that naproxen might be effective in ankylosing spondylitis. The initial trial was open, but at six months, a double-blind crossover \"placebo pulse\" was superimposed on the open trial. Thirty-six patients entered the trial taking a daily dose of 500 mg naproxen. At the end of the first month, 35 assessed naproxen as being equally effective to, or better than, previous therapy. The first ten patients to complete six months on naproxen took part in a placebo pulse study comprising two consecutive four-week periods, in one of which each patient took 500 mg naproxen, in the other, identical placebo capsules, the order being randomized. Eight patients correctly identified the placebo capsules (P=0.02). During the 16 months of trial, six patients have withdrawn, two being in remission and four for lack of efficacy. The remaining 30 patients have completed six months and 22 have completed at least 12 months on naproxen. At the end of six months pain was less (P=0.02), morning stiffness had decreased (P less than 0.01), and immobility stiffness had improved (P less than 0.01). These patients are impressed by the improvement in pain and stiffness and have little disability. All continue full-time employment and have been able to increase their leisure activities without discomfort. No persistent side effects were observed, and naproxen appears to be a useful drug in the treatment of anklyosing spondylitis."}
{"id": "PMID:1092730", "title": "The treatment of acute gout with naproxen.", "content": "The effectiveness of naproxen in the management of acute gouty arthritis was assessed in an open study of 20 patients. These patients were selected on the basis of their clinical presentation of characteristic acute arthritis associated in 19 with concomitant hyperuricemia. There were 17 men and three women varying in age from 35 to 89 years. The first 12 patients were treated with 600 mg naproxen initially, followed by 300 mg every 8 hours for the first 48 hours and then tapered or discontinued depending upon their clinical response. The last eight patients received a loading dose of 750 mg naproxen, followed by 250 mg every 8 hours for a duration of 72 hours before tapering the drug. The response of 15 of the 20 was either excellent or good, while the response was fair in three and poor in two. Poor responders had been failures in other regimens or were treated late in the course of their attack. The higher loading dose was associated with more rapid and satisfactory remission. No significant undesirable side effects were observed. On the basis of this study, naproxen was found effective in alleviating the inflammation of acute gout.", "contents": "The treatment of acute gout with naproxen. The effectiveness of naproxen in the management of acute gouty arthritis was assessed in an open study of 20 patients. These patients were selected on the basis of their clinical presentation of characteristic acute arthritis associated in 19 with concomitant hyperuricemia. There were 17 men and three women varying in age from 35 to 89 years. The first 12 patients were treated with 600 mg naproxen initially, followed by 300 mg every 8 hours for the first 48 hours and then tapered or discontinued depending upon their clinical response. The last eight patients received a loading dose of 750 mg naproxen, followed by 250 mg every 8 hours for a duration of 72 hours before tapering the drug. The response of 15 of the 20 was either excellent or good, while the response was fair in three and poor in two. Poor responders had been failures in other regimens or were treated late in the course of their attack. The higher loading dose was associated with more rapid and satisfactory remission. No significant undesirable side effects were observed. On the basis of this study, naproxen was found effective in alleviating the inflammation of acute gout."}
{"id": "PMID:1092731", "title": "Naproxen: corticosteroid-sparing effect in rheumatoid arthritis.", "content": "Ambulatory patients with a diagnosis of \"classical\" or \"definite\" active adult rheumatoid arthritis were selected. They had been stabilized for at least six months previously with oral administration of paramethasone acetate plus various analgesics and/or nonsteroidal anti-inflammatory agents. During an initial period of two to five weeks, the paramethasone dosage was adjusted and other medications replaced by placebo to find the minimum required to keep the patient at a level similar to the one at the beginning of the study. Subsequently, with the double-blind method, the effect of 400 mg naproxen daily was compared with placebo, making periodic and progressive reductions in the corticosteroid dosage, unknown to the patient, until a tolerable minimum was found. The average total observation time was 14 weeks. Results obtained in the first 28 patients showed an average reduction of 57 per cent in paramethasone dose for the group treated with naproxen, versus 21 per cent for the control group. One patient experienced euphoria which could possibly have been related to the test drug. It is concluded that naproxen possesses a significant corticosteroid-sparing effect with negligible toxicity.", "contents": "Naproxen: corticosteroid-sparing effect in rheumatoid arthritis. Ambulatory patients with a diagnosis of \"classical\" or \"definite\" active adult rheumatoid arthritis were selected. They had been stabilized for at least six months previously with oral administration of paramethasone acetate plus various analgesics and/or nonsteroidal anti-inflammatory agents. During an initial period of two to five weeks, the paramethasone dosage was adjusted and other medications replaced by placebo to find the minimum required to keep the patient at a level similar to the one at the beginning of the study. Subsequently, with the double-blind method, the effect of 400 mg naproxen daily was compared with placebo, making periodic and progressive reductions in the corticosteroid dosage, unknown to the patient, until a tolerable minimum was found. The average total observation time was 14 weeks. Results obtained in the first 28 patients showed an average reduction of 57 per cent in paramethasone dose for the group treated with naproxen, versus 21 per cent for the control group. One patient experienced euphoria which could possibly have been related to the test drug. It is concluded that naproxen possesses a significant corticosteroid-sparing effect with negligible toxicity."}
{"id": "PMID:1092732", "title": "An open trial of naproxen in rheumatoid arthritis patients with significant esophageal, gastric, and duodenal lesions.", "content": "To confirm the reported lack of major gastrointestinal side effects of naproxen, we gave 58 patients with active rheumatoid arthritis and significant gastrointestinal disease therapeutic doses of naproxen while closely monitoring them for signs and symptoms of gastrointestinal dysfunction. All patients underwent upper gastrointestinal x-ray examinations at the start of the trail, and, when indicated, during the course of the study. Endoscopies were also performed when indicated. Forty patients had hiatus hernia and 35 had peptic ulcer (23 duodenal ulcer and 12 gastric ulcer). Twenty-six patients had a combination of hiatus hernia with either type of peptic ulcer. After 262 patient visits over a period of 52 weeks, 35 patients remained in the study, all having had more than six months of naproxen therapy in dosages ranging from 500 to 750 mg daily. In 33 of the 35, the response to naproxen had generally been good to excellent. Only seven patients dropped out of the trial because of complaints referable to side effects. There were no major related upper gastrointestinal side effects as monitored by continuing clinical evaluation, stool occult blood, comprehensive laboratory examination, and, where indicated, upper gastrointestinal x-ray studies. Approximately 70 per cent of the patients demonstrated efficacy on long-term naproxen therapy by subjective and objective parameters. Naproxen appears to be an efficacious and remarkably safe drug in the long-term therapy of rheumatoid arthritis, even in the presence of significant upper gastrointestinal symptomatology.", "contents": "An open trial of naproxen in rheumatoid arthritis patients with significant esophageal, gastric, and duodenal lesions. To confirm the reported lack of major gastrointestinal side effects of naproxen, we gave 58 patients with active rheumatoid arthritis and significant gastrointestinal disease therapeutic doses of naproxen while closely monitoring them for signs and symptoms of gastrointestinal dysfunction. All patients underwent upper gastrointestinal x-ray examinations at the start of the trail, and, when indicated, during the course of the study. Endoscopies were also performed when indicated. Forty patients had hiatus hernia and 35 had peptic ulcer (23 duodenal ulcer and 12 gastric ulcer). Twenty-six patients had a combination of hiatus hernia with either type of peptic ulcer. After 262 patient visits over a period of 52 weeks, 35 patients remained in the study, all having had more than six months of naproxen therapy in dosages ranging from 500 to 750 mg daily. In 33 of the 35, the response to naproxen had generally been good to excellent. Only seven patients dropped out of the trial because of complaints referable to side effects. There were no major related upper gastrointestinal side effects as monitored by continuing clinical evaluation, stool occult blood, comprehensive laboratory examination, and, where indicated, upper gastrointestinal x-ray studies. Approximately 70 per cent of the patients demonstrated efficacy on long-term naproxen therapy by subjective and objective parameters. Naproxen appears to be an efficacious and remarkably safe drug in the long-term therapy of rheumatoid arthritis, even in the presence of significant upper gastrointestinal symptomatology."}
{"id": "PMID:1092733", "title": "Color-specific interconnections of cones and horizontal cells in the retina of the goldfish.", "content": "In Golgi preparations of goldfish retina we have observed three types of horizontal cell which receive exclusively from cones and one which receives exclusively from rods. The cone horizontal cells were designated H1, H2 and H3, in order of increasing dendritic spread, increasing separation from the outer synaptic layer, decreasing size of perikaryon, and decreasing density of cone contacts. Slender appendages with knobby terminal enlargements project  horizontal cells by alalyzing serial 1 mum sections with the light microscope. The probable inputs, in terms of visual pigments in the cones which contact them, are: H1, red+green+blue; H2, green+blue; H3, blue. Analysis of previously published work suggests (1) that H1 cells generate monophasic or L-type responses, H2 cells generate biphasic or C1-type responses, and H3 cells generate triphasic or C2-type responses; (2) that H1 cells receive direct functional input at least from red-sensitive cones, H2 cells from green-sensitive cones, and H3 cells from blue-sensitive cones, and (3) that H1 constitute pathways from cones to H2 cells, and H2 cells, and H2 cells constitute pathways from cones and H1 cells to H3 cells. The precise location and route of the transfers, from H1 to H2 and from H2 to H3, are not yet known.", "contents": "Color-specific interconnections of cones and horizontal cells in the retina of the goldfish. In Golgi preparations of goldfish retina we have observed three types of horizontal cell which receive exclusively from cones and one which receives exclusively from rods. The cone horizontal cells were designated H1, H2 and H3, in order of increasing dendritic spread, increasing separation from the outer synaptic layer, decreasing size of perikaryon, and decreasing density of cone contacts. Slender appendages with knobby terminal enlargements project  horizontal cells by alalyzing serial 1 mum sections with the light microscope. The probable inputs, in terms of visual pigments in the cones which contact them, are: H1, red+green+blue; H2, green+blue; H3, blue. Analysis of previously published work suggests (1) that H1 cells generate monophasic or L-type responses, H2 cells generate biphasic or C1-type responses, and H3 cells generate triphasic or C2-type responses; (2) that H1 cells receive direct functional input at least from red-sensitive cones, H2 cells from green-sensitive cones, and H3 cells from blue-sensitive cones, and (3) that H1 constitute pathways from cones to H2 cells, and H2 cells, and H2 cells constitute pathways from cones and H1 cells to H3 cells. The precise location and route of the transfers, from H1 to H2 and from H2 to H3, are not yet known."}
{"id": "PMID:1092734", "title": "Horizontal cell axons and axon terminals in goldfish retina.", "content": "Retinas of ordinary and black moor varieties of goldfish (Carassius auratus) were prepared by the Golgi method, mounted flat or sectioned vertically, and studied in the light microscope. Three types of horizontal cells whose dendrites contact only cones, and one type whose dendrites contact only rods, were observed. The cone horizontal cells (Cajal's \"external horizontal cells\") all have slender axons which descend gradually to the inner nuclear layer and terminate there in long, fusiform expansions (Cajal's \"internal horizontal cells\"). The thin and thick portions of the axons, as well as the perikarya of the horizontal cells, bear small numbers of straight, horizontally-directed, knobby filamentous appendages which may be sites of synaptic contact. The cone horizontal cell axons in goldfish, unlike those in higher vertebrates, do not terminate in contact with synaptic endings of photoreceptor cells, but in proximity to cells and processes deep in the inner nuclear layer. Axons have not yet been demonstrated on rod horizontal cells in goldfish.", "contents": "Horizontal cell axons and axon terminals in goldfish retina. Retinas of ordinary and black moor varieties of goldfish (Carassius auratus) were prepared by the Golgi method, mounted flat or sectioned vertically, and studied in the light microscope. Three types of horizontal cells whose dendrites contact only cones, and one type whose dendrites contact only rods, were observed. The cone horizontal cells (Cajal's \"external horizontal cells\") all have slender axons which descend gradually to the inner nuclear layer and terminate there in long, fusiform expansions (Cajal's \"internal horizontal cells\"). The thin and thick portions of the axons, as well as the perikarya of the horizontal cells, bear small numbers of straight, horizontally-directed, knobby filamentous appendages which may be sites of synaptic contact. The cone horizontal cell axons in goldfish, unlike those in higher vertebrates, do not terminate in contact with synaptic endings of photoreceptor cells, but in proximity to cells and processes deep in the inner nuclear layer. Axons have not yet been demonstrated on rod horizontal cells in goldfish."}
{"id": "PMID:1092735", "title": "The basilar pontine gray of the opossum: a correlated light and electron microscopic analysis.", "content": "Neurons within the basilar pontine gray (BPG) of the American opossum can be subdivided into four major nuclei which are named medial, lateral, ventral and peripeduncular in accordance with previous studies. In addition, several smaller subnuclei, such as the median and dorsolateral cell groups, are present, as well as two longitudinal columns of neurons within the ventral nucleus. Neurons in the BPG range in size from 9 to 35 mu and appear randomly distributed so that none of the subdivisions contains exclusively nerve cells of the same perikaryal dimension. Projection neurons as shown in Golgi impregnations have a variable dendritic pattern; those in peripeduncular zones exhibit dendrites closely applied to the surface of the cerebral peduncle, whereas those in other regions generally have a radial type of arrangement. Certain projection neurons can be distinguished on the basis of their dendritic surface, which bears either claw-like protrusions or stalked appendages. Smaller nerve cells measuring less than 18 mu may be intrinsic neurons, since axon-like processes arise from their dendrites and course for some distance near the parent cell before becoming thin and beaded. Ultrastructural observations show profiles of neurons comparable in size to those seen in Golgi impregnations and suggest at least four classes of presynaptic profiles. One category ranges in size from 2 to 8 mu, contains round vesicles (average diameter 450 A) and characteristically forms multiple asymmetric synaptic contacts with several small postsynaptic profiles, some of which appear to be the dendritic claws mentioned above. The other three types of axon terminals measure less than 2 mu in their greatest dimension and are distinguished by their synaptic vesicles; one group containing round vesicles with an average diameter of 380 A, a second group exhibiting larger round vesicles with an average diameter of 500 A and a third group containing flatened or eliptical vesicles. Transection of the superior cerebellar peduncle produces early filamentous and later electron dense degenerative changes in some, but not all, of the larger types of presynaptic profiles. Subsequent to large motor-sensory cortex ablations both filamentous and dark degenerating profiles are simultaneously observed at all survival times. In one case with a cortical lesion restricted to the motor-sensory cortex, mainly dark degenerating terminals are apparent in the ipsilateral pontine gray, whereas in a lesion confined to the visual cortex only filamentous degeneration was observed. It is suggested, therefore, that some of the dark degenerating profiles represent the terminals of collaterals of corticospinal axons and the filamentous boutons are terminal expansions of direct corticopontine fibers.", "contents": "The basilar pontine gray of the opossum: a correlated light and electron microscopic analysis. Neurons within the basilar pontine gray (BPG) of the American opossum can be subdivided into four major nuclei which are named medial, lateral, ventral and peripeduncular in accordance with previous studies. In addition, several smaller subnuclei, such as the median and dorsolateral cell groups, are present, as well as two longitudinal columns of neurons within the ventral nucleus. Neurons in the BPG range in size from 9 to 35 mu and appear randomly distributed so that none of the subdivisions contains exclusively nerve cells of the same perikaryal dimension. Projection neurons as shown in Golgi impregnations have a variable dendritic pattern; those in peripeduncular zones exhibit dendrites closely applied to the surface of the cerebral peduncle, whereas those in other regions generally have a radial type of arrangement. Certain projection neurons can be distinguished on the basis of their dendritic surface, which bears either claw-like protrusions or stalked appendages. Smaller nerve cells measuring less than 18 mu may be intrinsic neurons, since axon-like processes arise from their dendrites and course for some distance near the parent cell before becoming thin and beaded. Ultrastructural observations show profiles of neurons comparable in size to those seen in Golgi impregnations and suggest at least four classes of presynaptic profiles. One category ranges in size from 2 to 8 mu, contains round vesicles (average diameter 450 A) and characteristically forms multiple asymmetric synaptic contacts with several small postsynaptic profiles, some of which appear to be the dendritic claws mentioned above. The other three types of axon terminals measure less than 2 mu in their greatest dimension and are distinguished by their synaptic vesicles; one group containing round vesicles with an average diameter of 380 A, a second group exhibiting larger round vesicles with an average diameter of 500 A and a third group containing flatened or eliptical vesicles. Transection of the superior cerebellar peduncle produces early filamentous and later electron dense degenerative changes in some, but not all, of the larger types of presynaptic profiles. Subsequent to large motor-sensory cortex ablations both filamentous and dark degenerating profiles are simultaneously observed at all survival times. In one case with a cortical lesion restricted to the motor-sensory cortex, mainly dark degenerating terminals are apparent in the ipsilateral pontine gray, whereas in a lesion confined to the visual cortex only filamentous degeneration was observed. It is suggested, therefore, that some of the dark degenerating profiles represent the terminals of collaterals of corticospinal axons and the filamentous boutons are terminal expansions of direct corticopontine fibers."}
{"id": "PMID:1092736", "title": "The pattern of ocular dominance columns in macaque visual cortex revealed by a reduced silver stain.", "content": "A pattern of alternating dark and pale bands was observed in the striate cortex of the macaque monkey. The bands, which ran parallel to the surface, were seen in tangential sections stained with a reduced silver method for normal fibers and were most clear in layer 4C alpha, immediately deep to the line of Gennari. The dark bands were about 300 mu wide and showed blind endings and bifurcations. The light bands were about 50 mu wide and did not branch or terminate within area 17. Because the dark bands were similar in width to the bands of terminal degeneration which have been shown to result from single-layer lesions of the lateral geniculate body, it seemed possible that they corresponded to ocular dominance columns. To test this idea, the boundaries of ocular dominance columns were marked in a physiological experiment: tangential electrode penetrations were made in an anesthetized monkey and, as the electrode was advanced horizontally in the fourth layer, the eye preference of single units and of the background activity was monitored. Small electrolytic lesions were placed at the points where a change in eye preference occurred. The brain was subsequently fixed, sectioned tangentially and stained with the silver method. All the lesions--total of 12 --fell directly on the pale bands. Moreover, the electrode had not passed over any pale bands without a lesion being placed. It was concluded that the dark bands do correspond to single ocular dominance columns and the pale bands to the boundaries between columns. The banding appearance is due to a greater density of tangential fibers within columns than at the borders of columns. These tangential fibers are in part the preterminal arborizations of geniculocortical axons, since some of them have been shown to degenerate after geniculate lesions. The ocular dominance columns were mapped for most of the striate cortex, using serial tangential sections stained with the silver method. The overall pattern was ,imilar in several monkeys, though the details of the branching arrangements varied from animal to animal. The columns met the 17-18 border at right angles. On the outer surface of the hemisphere the columns converged from the 17-18 border, turned medially with repeated fusions of columns, and streamed over the lip of the calcarine fissure. In the roof of the fissure they met a second system of columns oriented parasagittally. In terms of the visual field, the columns ran roughly horizontally for the central 10 degrees of the field, and circumferentially beyond that. The columns were not mapped in the stem of the fissure, the area corresponding to the far periphery of the field. The constancy of column width across the cortex probably allows a functional matching between ocular-dominance and orientation columns.", "contents": "The pattern of ocular dominance columns in macaque visual cortex revealed by a reduced silver stain. A pattern of alternating dark and pale bands was observed in the striate cortex of the macaque monkey. The bands, which ran parallel to the surface, were seen in tangential sections stained with a reduced silver method for normal fibers and were most clear in layer 4C alpha, immediately deep to the line of Gennari. The dark bands were about 300 mu wide and showed blind endings and bifurcations. The light bands were about 50 mu wide and did not branch or terminate within area 17. Because the dark bands were similar in width to the bands of terminal degeneration which have been shown to result from single-layer lesions of the lateral geniculate body, it seemed possible that they corresponded to ocular dominance columns. To test this idea, the boundaries of ocular dominance columns were marked in a physiological experiment: tangential electrode penetrations were made in an anesthetized monkey and, as the electrode was advanced horizontally in the fourth layer, the eye preference of single units and of the background activity was monitored. Small electrolytic lesions were placed at the points where a change in eye preference occurred. The brain was subsequently fixed, sectioned tangentially and stained with the silver method. All the lesions--total of 12 --fell directly on the pale bands. Moreover, the electrode had not passed over any pale bands without a lesion being placed. It was concluded that the dark bands do correspond to single ocular dominance columns and the pale bands to the boundaries between columns. The banding appearance is due to a greater density of tangential fibers within columns than at the borders of columns. These tangential fibers are in part the preterminal arborizations of geniculocortical axons, since some of them have been shown to degenerate after geniculate lesions. The ocular dominance columns were mapped for most of the striate cortex, using serial tangential sections stained with the silver method. The overall pattern was ,imilar in several monkeys, though the details of the branching arrangements varied from animal to animal. The columns met the 17-18 border at right angles. On the outer surface of the hemisphere the columns converged from the 17-18 border, turned medially with repeated fusions of columns, and streamed over the lip of the calcarine fissure. In the roof of the fissure they met a second system of columns oriented parasagittally. In terms of the visual field, the columns ran roughly horizontally for the central 10 degrees of the field, and circumferentially beyond that. The columns were not mapped in the stem of the fissure, the area corresponding to the far periphery of the field. The constancy of column width across the cortex probably allows a functional matching between ocular-dominance and orientation columns."}
{"id": "PMID:1092739", "title": "Structure and stability of casein micelles.", "content": "The structure and stability of casein micelles are determined in large measure by the amino acid sequences of the constituent alphas1-, beta-, and kappa- caseins. In this paper we review, and attempt to connect with sequence data where possible: (1) molecular weight, structure, and dissociation of casein micelles; (2) physical characteristics of monomeric caseins, especially the distrubition of charged and hydrophobic residues and the possible occurrence of helical regions; and (3) forces involved in association of monomers.", "contents": "Structure and stability of casein micelles. The structure and stability of casein micelles are determined in large measure by the amino acid sequences of the constituent alphas1-, beta-, and kappa- caseins. In this paper we review, and attempt to connect with sequence data where possible: (1) molecular weight, structure, and dissociation of casein micelles; (2) physical characteristics of monomeric caseins, especially the distrubition of charged and hydrophobic residues and the possible occurrence of helical regions; and (3) forces involved in association of monomers."}
{"id": "PMID:1092744", "title": "A patient survey of denture tolerance before and after a mandibular vestibuloplasty with skin grafting.", "content": "Thirty-six patients were examined by two prosthodontistis after a mandibular vestibuloplasty with skin grafting to determine whether there was a significant improvement in these patients ability to tolerate their dentures. Previous published reports have been made only by oral surgeons. The results indicate that this procedure is a reliable treatment modality for patients who complain of looseness, poor masticatory function, and pain.", "contents": "A patient survey of denture tolerance before and after a mandibular vestibuloplasty with skin grafting. Thirty-six patients were examined by two prosthodontistis after a mandibular vestibuloplasty with skin grafting to determine whether there was a significant improvement in these patients ability to tolerate their dentures. Previous published reports have been made only by oral surgeons. The results indicate that this procedure is a reliable treatment modality for patients who complain of looseness, poor masticatory function, and pain."}
{"id": "PMID:1092746", "title": "Hospital dental service: a clinical evaluation to determine its justification.", "content": "A research study was undertaken to determine the importance of a hospital dental service in civilian hospitals. Two hundred hospitalized patients were evaluated to determine their oral health status and possible effects of poor oral health on related physical situations. Both cental caries and periodontal disease were prelavlent in the hospital patient population, and 83.5% of the patients suffered from some type of oral pathosis that required treatment. Hospital dental care is important, not only for the health and improvement of the total patient but also for the hospital to provide complete patient care.", "contents": "Hospital dental service: a clinical evaluation to determine its justification. A research study was undertaken to determine the importance of a hospital dental service in civilian hospitals. Two hundred hospitalized patients were evaluated to determine their oral health status and possible effects of poor oral health on related physical situations. Both cental caries and periodontal disease were prelavlent in the hospital patient population, and 83.5% of the patients suffered from some type of oral pathosis that required treatment. Hospital dental care is important, not only for the health and improvement of the total patient but also for the hospital to provide complete patient care."}
{"id": "PMID:1092748", "title": "Management of gallstones in the aged.", "content": "Calculous gallbladder disease is the most common indication for intra-abdominal surgery in the elderly.  Feeding bile salts may possibly increase the solubility of cholesterol in bile and may cause diminution in the size of radiolucent gallstones, the principal ingredient of which is cholesterol.  However, indications for such treatment are still not clear, and long-term effectiveness and safety still have to be evaluated.  If the aged patient with gallstones has severe symptoms, is a good medical risk and is not demented, surgical treatment should not be denied because of advanced age.  If the patient has \"silent\" or \"dyspeptic\" gallstones and is a poor risk or demented, surgical therapy should be postponed at least temporarily, in the hope that medical methods for dissolving gallstones soon will be perfected.", "contents": "Management of gallstones in the aged. Calculous gallbladder disease is the most common indication for intra-abdominal surgery in the elderly.  Feeding bile salts may possibly increase the solubility of cholesterol in bile and may cause diminution in the size of radiolucent gallstones, the principal ingredient of which is cholesterol.  However, indications for such treatment are still not clear, and long-term effectiveness and safety still have to be evaluated.  If the aged patient with gallstones has severe symptoms, is a good medical risk and is not demented, surgical treatment should not be denied because of advanced age.  If the patient has \"silent\" or \"dyspeptic\" gallstones and is a poor risk or demented, surgical therapy should be postponed at least temporarily, in the hope that medical methods for dissolving gallstones soon will be perfected."}
{"id": "PMID:1092749", "title": "Withdrawn, apathetic geriatric patients responsive to methylphenidate.", "content": "A double-blind randomized study showed that methylphenidate, as compared to placebo, was singularly effective in 44 withdrawn, apathetic geriatric patients.  Study selection and exclusion criteria were rigidly enforced.  Patients receiving methylphenidate in a dosage of 20 mg daily improved significantly over a period of six weeks as measured by results of tests for mental status, ward behavior (nursis' rating), target-symptom response, and physician's and nurses' global evaluations.  For this reason and because there were no side effects, this form of antidepressant therapy should be considered in the treatment of previously unresponsive or oversedated patients.", "contents": "Withdrawn, apathetic geriatric patients responsive to methylphenidate. A double-blind randomized study showed that methylphenidate, as compared to placebo, was singularly effective in 44 withdrawn, apathetic geriatric patients.  Study selection and exclusion criteria were rigidly enforced.  Patients receiving methylphenidate in a dosage of 20 mg daily improved significantly over a period of six weeks as measured by results of tests for mental status, ward behavior (nursis' rating), target-symptom response, and physician's and nurses' global evaluations.  For this reason and because there were no side effects, this form of antidepressant therapy should be considered in the treatment of previously unresponsive or oversedated patients."}
{"id": "PMID:1092753", "title": "Quantitation of isolated rat islets of Langerhans on the basis of deoxyribonucleic acid content under metabolic conditions of altered protein synthesis.", "content": "A micromethod was employed to estimate quantitatively and reproducibly the deoxyribonucleic acid (DNA) content of isolated rat islets of Langerhans. The DNA content per islet varied linearly with the mean diameter or the dry weight of the islets isolated. The DNA in the freeze-dried islets of male Sprague-Dawley or Wistar rats was about 21.0 mug/mug islet dry weight. Three to four weeks after hypophysectomy, with or without short term bovine growth hormone replacement, the DNA content per unit dry weight of islets was not significantly altered. Islet DNA content and islet dry weight are proposed as an interconvertible and reliable basis of reference for measurements of different islet functions.", "contents": "Quantitation of isolated rat islets of Langerhans on the basis of deoxyribonucleic acid content under metabolic conditions of altered protein synthesis. A micromethod was employed to estimate quantitatively and reproducibly the deoxyribonucleic acid (DNA) content of isolated rat islets of Langerhans. The DNA content per islet varied linearly with the mean diameter or the dry weight of the islets isolated. The DNA in the freeze-dried islets of male Sprague-Dawley or Wistar rats was about 21.0 mug/mug islet dry weight. Three to four weeks after hypophysectomy, with or without short term bovine growth hormone replacement, the DNA content per unit dry weight of islets was not significantly altered. Islet DNA content and islet dry weight are proposed as an interconvertible and reliable basis of reference for measurements of different islet functions."}
{"id": "PMID:1092754", "title": "Study of rickettsioses in Slovakia. I. Coxiella burneti and Rickettsiae of the spotted fever (SF) group in ticks and serological surveys in animals and humans in certain selected localities in the Lucenec and V. Krt\u00eds districts.", "content": "A study of the natural focus character of Q fever and the rickettsiosis caused by organisms of the SF group carried out in 1971-1973 in 7 selected villages in the Lucenec district and in a few control villages in the V. Krtis district, produced the following results: Among a total number of 2,200 ticks belonging to the species Dermacentor marginatus, Ixodes ricinus and Haemaphysalis punctata and tested for the presence of rickettsial organisms by the haemocyte test, C. burneti was detected in 8 from the chosen localities--in 2 females and 1 male of D. marginatus in Luborec, in 1 2. ricinus female in Maskova and in 1 female and 3 males of I. ricinus in Pincina--and in 5 from the control localities--in 1 D. marginatus female in Prebelce and in 3 females and 1 male of D. marginatus in V. Lom.", "contents": "Study of rickettsioses in Slovakia. I. Coxiella burneti and Rickettsiae of the spotted fever (SF) group in ticks and serological surveys in animals and humans in certain selected localities in the Lucenec and V. Krt\u00eds districts. A study of the natural focus character of Q fever and the rickettsiosis caused by organisms of the SF group carried out in 1971-1973 in 7 selected villages in the Lucenec district and in a few control villages in the V. Krtis district, produced the following results: Among a total number of 2,200 ticks belonging to the species Dermacentor marginatus, Ixodes ricinus and Haemaphysalis punctata and tested for the presence of rickettsial organisms by the haemocyte test, C. burneti was detected in 8 from the chosen localities--in 2 females and 1 male of D. marginatus in Luborec, in 1 2. ricinus female in Maskova and in 1 female and 3 males of I. ricinus in Pincina--and in 5 from the control localities--in 1 D. marginatus female in Prebelce and in 3 females and 1 male of D. marginatus in V. Lom."}
{"id": "PMID:1092755", "title": "New concepts on tetanus immunization: naturally acquired immunity.", "content": "Comments were made on misinterpretations concerning the natural resistance and natural immunization against tetanus. Arguments were given explaining why the disease itself did not determine adequate immunity. When, however, adequate conditions appear, tetanus toxin is known to stimulate the immune system and produce detectable humoral antibodies. Various possibilities resulting from the postulated harboring of tetanus bacilli by the human body and their eventual toxin production were analyzed and related to the human tetanus pathology. The existence of natural immunization was unquestionably demonstrated by presence of protective levels of tetanus antitoxin in the blood of the majority of 59 surveyed subjects considering that none of them had ever received any tetanus toxoid and most of them never received a single shot of any drug. The results of this survey originated a few arguments that may support the answer to some still intriguing phenomenona such as: 1. The relatively small number of cases of overt disease among people and animals born and living in large tetanus-risk regions all over the world. 2. The existence of \"poor responders\" and \"good responders\" to the primary tetanus toxoid stimulus. 3. The age distribution of tetanus showing evident prevalence among newborns and children. 4. The wide individual variations in the clinical picture of human tetanus as indicated by the localization and limitation of the symptoms and their severity.", "contents": "New concepts on tetanus immunization: naturally acquired immunity. Comments were made on misinterpretations concerning the natural resistance and natural immunization against tetanus. Arguments were given explaining why the disease itself did not determine adequate immunity. When, however, adequate conditions appear, tetanus toxin is known to stimulate the immune system and produce detectable humoral antibodies. Various possibilities resulting from the postulated harboring of tetanus bacilli by the human body and their eventual toxin production were analyzed and related to the human tetanus pathology. The existence of natural immunization was unquestionably demonstrated by presence of protective levels of tetanus antitoxin in the blood of the majority of 59 surveyed subjects considering that none of them had ever received any tetanus toxoid and most of them never received a single shot of any drug. The results of this survey originated a few arguments that may support the answer to some still intriguing phenomenona such as: 1. The relatively small number of cases of overt disease among people and animals born and living in large tetanus-risk regions all over the world. 2. The existence of \"poor responders\" and \"good responders\" to the primary tetanus toxoid stimulus. 3. The age distribution of tetanus showing evident prevalence among newborns and children. 4. The wide individual variations in the clinical picture of human tetanus as indicated by the localization and limitation of the symptoms and their severity."}
{"id": "PMID:1092756", "title": "Complex surveillance of Streptococcus pyogenes. I. Immunological surveys of anti-M antibodies and possibilities of long-term epidemiological prognosis.", "content": "The results of complex surveillance of Streptococcus pyogenes, based on mass infections in the Czechoslovak army over a period of 11 years, are submitted. The epidemiological method revealed an incidence of cycles, lasting several years, of predominant pairs of T types, most of which could not be typed by their M protein. Auxiliary immunological surveys allowed the biological M activity of widespread strains to be estimated and probably form a basis for considerations on a negative long-term epidemiological type prognosis. The preliminary results presented offer prospects for a realistic methodological approach to the question of the type content of a future antistreptococcal vaccine.", "contents": "Complex surveillance of Streptococcus pyogenes. I. Immunological surveys of anti-M antibodies and possibilities of long-term epidemiological prognosis. The results of complex surveillance of Streptococcus pyogenes, based on mass infections in the Czechoslovak army over a period of 11 years, are submitted. The epidemiological method revealed an incidence of cycles, lasting several years, of predominant pairs of T types, most of which could not be typed by their M protein. Auxiliary immunological surveys allowed the biological M activity of widespread strains to be estimated and probably form a basis for considerations on a negative long-term epidemiological type prognosis. The preliminary results presented offer prospects for a realistic methodological approach to the question of the type content of a future antistreptococcal vaccine."}
{"id": "PMID:1092757", "title": "Thymic involution: effect on T cell differentiation.", "content": "The thymus of long-lived BC3F mice involutes progressively throughout life, beginning at 6 weeks of age. This is manifested by the loss of cortical lymphoid mass and by the degenerative changes in epithelial cells. The purpose of this study was to determine to what extent age-related degenerative changes of the thymus affect its capacity to influence the maturation of thymic-derived (T) cells. Accordingly, thymic lobes of mice ranging in age from 1 day to 33 months were implanted under the kidney capsule of T cell-deprived syngeneic young adult TXB mice, and the emergence of T cells was assessed kinetically by various morphologic and functional indices which may be reflective of different T cell subpopulations. They are: a) histology of the thymsu graft, b) lymphocyte repopulation of the T cell-dependent areas of lymph nodes, c) total number of splenic lymphocytes carrying theta antigens (theta-+), d) T cell-dependent humoral immune response and e) proliferative response of splenic cells to plant lectins, phytohemagglutinin (PHA) and succinyl-concanavalin A (s-Con A), and allogeneic lymphocytes. The results revealed that the T cell-transforming influence of thymic tissues generally decreases with increasing age. The difference in the patterns of recovery of the various indices of thymus-grafted TXB mice suggests that the extent to which T cells can mature is dependent upon the degree of involution the thymic tissue has undergone with age. In particular thymic tissues lose the capacity to influence the following functions with advancing age: 1) lymphocyte repopulation of the T cell-dependent areas of lymph nodes; 2) mitogenic reactivity of splenic cells to T cell-specific mitogens (PHA and s-Con A): 3) number of splenic theta-+ lymphocytes and splenic T cell helper function; and 4) mitogenic reactivity of splenic T cells to allogeneic lymphocytes.", "contents": "Thymic involution: effect on T cell differentiation. The thymus of long-lived BC3F mice involutes progressively throughout life, beginning at 6 weeks of age. This is manifested by the loss of cortical lymphoid mass and by the degenerative changes in epithelial cells. The purpose of this study was to determine to what extent age-related degenerative changes of the thymus affect its capacity to influence the maturation of thymic-derived (T) cells. Accordingly, thymic lobes of mice ranging in age from 1 day to 33 months were implanted under the kidney capsule of T cell-deprived syngeneic young adult TXB mice, and the emergence of T cells was assessed kinetically by various morphologic and functional indices which may be reflective of different T cell subpopulations. They are: a) histology of the thymsu graft, b) lymphocyte repopulation of the T cell-dependent areas of lymph nodes, c) total number of splenic lymphocytes carrying theta antigens (theta-+), d) T cell-dependent humoral immune response and e) proliferative response of splenic cells to plant lectins, phytohemagglutinin (PHA) and succinyl-concanavalin A (s-Con A), and allogeneic lymphocytes. The results revealed that the T cell-transforming influence of thymic tissues generally decreases with increasing age. The difference in the patterns of recovery of the various indices of thymus-grafted TXB mice suggests that the extent to which T cells can mature is dependent upon the degree of involution the thymic tissue has undergone with age. In particular thymic tissues lose the capacity to influence the following functions with advancing age: 1) lymphocyte repopulation of the T cell-dependent areas of lymph nodes; 2) mitogenic reactivity of splenic cells to T cell-specific mitogens (PHA and s-Con A): 3) number of splenic theta-+ lymphocytes and splenic T cell helper function; and 4) mitogenic reactivity of splenic T cells to allogeneic lymphocytes."}
{"id": "PMID:1092758", "title": "Development of B lymphocytes. I. Cell populations and a critical event during ontogeny.", "content": "The ontogeny of Ig(+), theta(+) and \"null\" (Ig-, theta-) lymphocytes in the mouse spleen was followed. An event was found at about 10 days after birth when the percentage of Ig(+) lymphocytes in the spleen begins to rise sharply, and the percentage of \"null\" lymphocytes falls. The Ig(+) lymphocytes before and after this event were then evaluated by four criteria pertaining to B cells. Three of these, the presence of C3 receptors, \"capping\" behavior, and the amount and/or disposition of surface Ig distinguished the Ig(+) lymphocytes before the event described from those present afterward. The fourth criterion, the presence of Fc receptors, did not distinguish between the early and mature forms of Ig(+) lymphocytes.", "contents": "Development of B lymphocytes. I. Cell populations and a critical event during ontogeny. The ontogeny of Ig(+), theta(+) and \"null\" (Ig-, theta-) lymphocytes in the mouse spleen was followed. An event was found at about 10 days after birth when the percentage of Ig(+) lymphocytes in the spleen begins to rise sharply, and the percentage of \"null\" lymphocytes falls. The Ig(+) lymphocytes before and after this event were then evaluated by four criteria pertaining to B cells. Three of these, the presence of C3 receptors, \"capping\" behavior, and the amount and/or disposition of surface Ig distinguished the Ig(+) lymphocytes before the event described from those present afterward. The fourth criterion, the presence of Fc receptors, did not distinguish between the early and mature forms of Ig(+) lymphocytes."}
{"id": "PMID:1092759", "title": "Rejection of mammary adenocarcinoma cell tumors in DBA/2 mice immunocompromised by thymectomy and treatment with antithymocyte serum.", "content": "Syngeneic mammary adenocarcinoma cells were mixed with  killed Corynebacterium parvum organisms and were then transplanted subcutaneously into groups of normal and immunocompromised mice. The tumors formed at the site of injection exhibited normal growth for approximately 12 days followed by rapid and lasting rejection in both normal and immunocompromised animals. The control mice were protected against reinjections of 10-4 to 10-8 cells of the same line of tumor cells. The immunocompromised mice exhibited protection to reinjection of 10-4 cells of the tumor line initially employed. Microscopic examination of histologic sections of 10-day-old tumors in immunocompromised mice arising from administration of tumor cells and C.parvum mixtures revealed infiltration of only macrophages whereas those from normal animals exhibited both macrophages and lymphocytes. The data suggested that primary rejection of these tumors in immunocompromised mice may be the result of macrophage activity together with humoral immunity. These studies also revealed that 59 to 75% of immunocompromised mice exhibit protection against reinjection of the same line of tumor cells if twice-weekly treatment (i.p.) with killed C. parvum was included after inoculation of tumor cells mixed with C. parvum vaccine. These mice, unlike their saline-treated peers, exhibit delayed hypersensitivity reactions which are associated with proliferation of theta antigen-bearing lymphocytes. They also have intact tumor-directed humoral immune reactions.", "contents": "Rejection of mammary adenocarcinoma cell tumors in DBA/2 mice immunocompromised by thymectomy and treatment with antithymocyte serum. Syngeneic mammary adenocarcinoma cells were mixed with  killed Corynebacterium parvum organisms and were then transplanted subcutaneously into groups of normal and immunocompromised mice. The tumors formed at the site of injection exhibited normal growth for approximately 12 days followed by rapid and lasting rejection in both normal and immunocompromised animals. The control mice were protected against reinjections of 10-4 to 10-8 cells of the same line of tumor cells. The immunocompromised mice exhibited protection to reinjection of 10-4 cells of the tumor line initially employed. Microscopic examination of histologic sections of 10-day-old tumors in immunocompromised mice arising from administration of tumor cells and C.parvum mixtures revealed infiltration of only macrophages whereas those from normal animals exhibited both macrophages and lymphocytes. The data suggested that primary rejection of these tumors in immunocompromised mice may be the result of macrophage activity together with humoral immunity. These studies also revealed that 59 to 75% of immunocompromised mice exhibit protection against reinjection of the same line of tumor cells if twice-weekly treatment (i.p.) with killed C. parvum was included after inoculation of tumor cells mixed with C. parvum vaccine. These mice, unlike their saline-treated peers, exhibit delayed hypersensitivity reactions which are associated with proliferation of theta antigen-bearing lymphocytes. They also have intact tumor-directed humoral immune reactions."}
{"id": "PMID:1092760", "title": "Pathogenesis of rabies in immunodeficient mice.", "content": "The HEP and ts2 strains of rabies virus inoculated intracerebrally into adult mice normally cause clinically inapparent infection. In the experiments described, this is converted into a lethal infection by immunosuppression with cyclophosphamide, which also prevented induction of immunity with vaccine. Lethal infection of HEP-inoculated mice was also observed in mice treated with antihymocytic serum, and in athymic (BALB/c-nu) nude mice.", "contents": "Pathogenesis of rabies in immunodeficient mice. The HEP and ts2 strains of rabies virus inoculated intracerebrally into adult mice normally cause clinically inapparent infection. In the experiments described, this is converted into a lethal infection by immunosuppression with cyclophosphamide, which also prevented induction of immunity with vaccine. Lethal infection of HEP-inoculated mice was also observed in mice treated with antihymocytic serum, and in athymic (BALB/c-nu) nude mice."}
{"id": "PMID:1092761", "title": "Immunoglobulin biosynthesis in myeloma-associated and casein- and endotoxin-induced murine amyloidosis.", "content": "Amyloidosis was induced in C57BL mice by daily injections of casein and in BALB/c mice by daily injections of endotoxin. There was no obvious disorder of immunoglobulin biosynthesis by spleen lymphocytes in these mice either before, during the development of, or in the amyloidotic stage. The pattern of immunoglobulin synthesis, assembly, and secretion was unaltered, the relative amount of heavy and light chains produced was normal, and there was an absence of immunoglobulin polypeptide chain fragments. Small amounts of amyloid were present in only 1 of 19 BALB/c and C3H mice (the IgG2a producing MOPC 173 tumor) bearing immunoglobulin-producing myeloma tumors and variants of these tumors. There was no relationship between excess light chain production by tumor plasma cells or spleen lymphocytes and the development of amyloidosis and there were no light chain fragments demonstrable. Antiserum prepared against casein-induced amyloid cross-reacted by immunofluorescence with the amyloid present in the MOPC 173 tumor-bearing mice, indicating the presence of common antigenic determinants in these two forms of amyloid. Attempts to study the biosynthesis of amyloid with incorporation of radioactively labeled amino acids were unsucessful.", "contents": "Immunoglobulin biosynthesis in myeloma-associated and casein- and endotoxin-induced murine amyloidosis. Amyloidosis was induced in C57BL mice by daily injections of casein and in BALB/c mice by daily injections of endotoxin. There was no obvious disorder of immunoglobulin biosynthesis by spleen lymphocytes in these mice either before, during the development of, or in the amyloidotic stage. The pattern of immunoglobulin synthesis, assembly, and secretion was unaltered, the relative amount of heavy and light chains produced was normal, and there was an absence of immunoglobulin polypeptide chain fragments. Small amounts of amyloid were present in only 1 of 19 BALB/c and C3H mice (the IgG2a producing MOPC 173 tumor) bearing immunoglobulin-producing myeloma tumors and variants of these tumors. There was no relationship between excess light chain production by tumor plasma cells or spleen lymphocytes and the development of amyloidosis and there were no light chain fragments demonstrable. Antiserum prepared against casein-induced amyloid cross-reacted by immunofluorescence with the amyloid present in the MOPC 173 tumor-bearing mice, indicating the presence of common antigenic determinants in these two forms of amyloid. Attempts to study the biosynthesis of amyloid with incorporation of radioactively labeled amino acids were unsucessful."}
{"id": "PMID:1092765", "title": "Protective studies with a group A streptococcal M protein vaccine. II. Challange of volenteers after local immunization in the upper respiratory tract.", "content": "Twenty-one adult volunteers were immunized at monthly intervals with three doses of purified type 1 M protein of group A Streptococcus. The soluble vaccine in buffer was administered by aerosol spray into the nares and oropharynx; 23 control subjects received a buffer placebo in the same manner. Antibody responses were observed in sera and nasal washings of some but not all vaccines. Approximately 30 days after  the last dose,  all subjects were challenged with homologus streptococci applied by swab to the phayngeal-tonsillar areas. In a double-blind system of evaluation, physical signs and symptoms were followed for assessment of infection. Illness was  defined on the basis of a positive throat culture, fever, a twofold increase in white blood cell count over baseline, exudative pharyngitis, and cervical adenopathy. By these criteria four vaccinees and 11 controls were obviously ill. One vaccinee and six controls were questionably ill, fulfilling some but not all of the criteria. sixteen vaccinees and six controls were not ill (P less than 0.001). Positive throat cultures were observed in five vaccines and 19 controls (P less than 0.001). Penicillin was administered five days after challenge. No poststreptoccal sequelae or other complication were observed. Thus local immunization with M protein apparently can prevent both colonization and clinical illness after challenge with homologous streptococci.", "contents": "Protective studies with a group A streptococcal M protein vaccine. II. Challange of volenteers after local immunization in the upper respiratory tract. Twenty-one adult volunteers were immunized at monthly intervals with three doses of purified type 1 M protein of group A Streptococcus. The soluble vaccine in buffer was administered by aerosol spray into the nares and oropharynx; 23 control subjects received a buffer placebo in the same manner. Antibody responses were observed in sera and nasal washings of some but not all vaccines. Approximately 30 days after  the last dose,  all subjects were challenged with homologus streptococci applied by swab to the phayngeal-tonsillar areas. In a double-blind system of evaluation, physical signs and symptoms were followed for assessment of infection. Illness was  defined on the basis of a positive throat culture, fever, a twofold increase in white blood cell count over baseline, exudative pharyngitis, and cervical adenopathy. By these criteria four vaccinees and 11 controls were obviously ill. One vaccinee and six controls were questionably ill, fulfilling some but not all of the criteria. sixteen vaccinees and six controls were not ill (P less than 0.001). Positive throat cultures were observed in five vaccines and 19 controls (P less than 0.001). Penicillin was administered five days after challenge. No poststreptoccal sequelae or other complication were observed. Thus local immunization with M protein apparently can prevent both colonization and clinical illness after challenge with homologous streptococci."}
{"id": "PMID:1092766", "title": "The diffusion and concentration of trimethoprim in human vaginal fluid.", "content": "Colonization of the vaginal introitus with Enterobacteriaceae was cleared dramatically in women receiving trimethoprim-sulfamethoxazole for recurrent urinary tract infections. Vaginal fluid was collected from seven women; five of the seven had two vaginal fluid collections each while on full-dose therapy (total, 10 samples), and five had two or more collections each while on prophylactic, low-dose therapy (total, 14 samples). Bactericidal concentrations of trimethoprim were found in all samples of vaginal fluid, often exceeding the serum level by severalfold. Sulfamethoxazole was either undetectable or present only in fractional concentrations.", "contents": "The diffusion and concentration of trimethoprim in human vaginal fluid. Colonization of the vaginal introitus with Enterobacteriaceae was cleared dramatically in women receiving trimethoprim-sulfamethoxazole for recurrent urinary tract infections. Vaginal fluid was collected from seven women; five of the seven had two vaginal fluid collections each while on full-dose therapy (total, 10 samples), and five had two or more collections each while on prophylactic, low-dose therapy (total, 14 samples). Bactericidal concentrations of trimethoprim were found in all samples of vaginal fluid, often exceeding the serum level by severalfold. Sulfamethoxazole was either undetectable or present only in fractional concentrations."}
{"id": "PMID:1092767", "title": "Disseminated candidiasis. i. an experimental model in the guinea pig.", "content": "A model of experimental disseminated candidiasis was developed in the guinea pig; Candida albicans was injected intraperitoneally or intravenously. The kidney was the most severely affected organ, with maximal colony counts of 26,208 plus or minus 9,152 colony-forming units/g of tissue five days after a sublethal inocluation (one-sixth of the 50% lethal dose) of viable organisms. The heart was the next most severely affected organ, and other organs were affected little. Histologic studies confirmed the data from colony counts and showed a progression of inflammation similar to that seen in human disease. A leukocytosis predominantly of mononuclear cells, and to a lesser degree of granulocytes, occurred and was maximal four days after inoculation. This model will provide the framework for a series of future studies of the various factors important in natural host defense against infection with Candida, as well as the effects of various immunosuppressive agents, particularly corticosteroids, on the development and expression of immunity and protection against infection.", "contents": "Disseminated candidiasis. i. an experimental model in the guinea pig. A model of experimental disseminated candidiasis was developed in the guinea pig; Candida albicans was injected intraperitoneally or intravenously. The kidney was the most severely affected organ, with maximal colony counts of 26,208 plus or minus 9,152 colony-forming units/g of tissue five days after a sublethal inocluation (one-sixth of the 50% lethal dose) of viable organisms. The heart was the next most severely affected organ, and other organs were affected little. Histologic studies confirmed the data from colony counts and showed a progression of inflammation similar to that seen in human disease. A leukocytosis predominantly of mononuclear cells, and to a lesser degree of granulocytes, occurred and was maximal four days after inoculation. This model will provide the framework for a series of future studies of the various factors important in natural host defense against infection with Candida, as well as the effects of various immunosuppressive agents, particularly corticosteroids, on the development and expression of immunity and protection against infection."}
{"id": "PMID:1092768", "title": "Isolation and characterization of Gal E mutant Ty 21a of Salmonella typhi: a candidate strain for a live, oral typhoid vaccine.", "content": "A gal E mutant of Salmonella typhi was isolated; results obtained with Salmonella typhimurium and the mouse as a model for human typhoid fever indicated that this mutant has the potential for use as a live, oral typhoid vaccine. The mutant, Ty 21a, took up galactose from exogenous sources and accumulated sufficient quantities of galactose-1-phosphate and uridine diphosphate galactose to cause lysis of the cells, an event that resulted in the avirulence of the strain. Galactose was incorporated sufficiently into the cell wall of Ty 21a to allow the synthesis of smooth-type lipopolysaccharides, which are necessary for the proper immunogenicity. Cells of strain Ty 21a, when given intraperitoneally, protected mice against lethal challenge with strain Ty 2 of S. typhi.", "contents": "Isolation and characterization of Gal E mutant Ty 21a of Salmonella typhi: a candidate strain for a live, oral typhoid vaccine. A gal E mutant of Salmonella typhi was isolated; results obtained with Salmonella typhimurium and the mouse as a model for human typhoid fever indicated that this mutant has the potential for use as a live, oral typhoid vaccine. The mutant, Ty 21a, took up galactose from exogenous sources and accumulated sufficient quantities of galactose-1-phosphate and uridine diphosphate galactose to cause lysis of the cells, an event that resulted in the avirulence of the strain. Galactose was incorporated sufficiently into the cell wall of Ty 21a to allow the synthesis of smooth-type lipopolysaccharides, which are necessary for the proper immunogenicity. Cells of strain Ty 21a, when given intraperitoneally, protected mice against lethal challenge with strain Ty 2 of S. typhi."}
{"id": "PMID:1092769", "title": "Production and characterization of exotoxin(s) of Shigella dysenteriae type 1.", "content": "A semicontinuous fermenter system was developed in which broth culture filtrates of Shigella dysenteriae type 1 yielded substantial amounts of exotoxin. Biologic activity of the exotoxin was characterized by means of three assays: the rabbit ileal loop for fluid evocation (enterotoxicity), mouse lethality after parenteral injection (neurotoxicity), and HeLa cell toxicity in vitro (cytotoxicity). Although the culture filtrate was highly active, disc electrophoresis revealed that the toxin is a minor component of the mixture of proteins in the crude preparation, and that the minor representation contrasts with the relative prominence of exotoxins in cultures of other bacteria, such as Vibrio cholerae, Corynebacterium diphtheriae, and Clostridium tetani. Filtrate toxin, when purified by isoelectric focusing in polyacrylamide gel, was found to contain two toxic moieties. One was resolvable as a single band with an isoelectric joint (pI) of 7.25, a molecular weight of 72,000, and all three types of biological activity. The second moiety was isoelectric at pH 6.00, contained two subcomponents, and further contrasted with the pI 7.25 toxin by being more cytotoxic while being devoid of enteroneurotoxic activity.", "contents": "Production and characterization of exotoxin(s) of Shigella dysenteriae type 1. A semicontinuous fermenter system was developed in which broth culture filtrates of Shigella dysenteriae type 1 yielded substantial amounts of exotoxin. Biologic activity of the exotoxin was characterized by means of three assays: the rabbit ileal loop for fluid evocation (enterotoxicity), mouse lethality after parenteral injection (neurotoxicity), and HeLa cell toxicity in vitro (cytotoxicity). Although the culture filtrate was highly active, disc electrophoresis revealed that the toxin is a minor component of the mixture of proteins in the crude preparation, and that the minor representation contrasts with the relative prominence of exotoxins in cultures of other bacteria, such as Vibrio cholerae, Corynebacterium diphtheriae, and Clostridium tetani. Filtrate toxin, when purified by isoelectric focusing in polyacrylamide gel, was found to contain two toxic moieties. One was resolvable as a single band with an isoelectric joint (pI) of 7.25, a molecular weight of 72,000, and all three types of biological activity. The second moiety was isoelectric at pH 6.00, contained two subcomponents, and further contrasted with the pI 7.25 toxin by being more cytotoxic while being devoid of enteroneurotoxic activity."}
{"id": "PMID:1092770", "title": "Involvement of the upper respiratory tract in orally induced salmonellosis in mice.", "content": "The possible involvement of the tissues of the head and neck during the early pathogenesis of orally or intragastrically induced murine salmonellosis was examined. Viable counts of Salmonella in the local lymphoid tissues and draining nodes indicated that the ileal Peyer's patches were primarily involved in the development of a subsequent enteric fever, irrespective of the method of oral challenge. Contamination of drinking water with a high concentration of salmonellae produced infection of the ileal Peyers patches and the eventual development of systemic disease. The cervical lymph nodes were sporadically involved early in the infection, but this involvement soon became general, with large numbers of salmonellae in the cervical nodes by 36 hr. A lower concentration of salmonellae in drinking water led to primary involvement of the ileal Peyers patches; upper respiratory tract involvement was detected much later in the infection. Intragastrically invected mice exhibited the same low-level infection of the cervical lymph nodes as that in mice infected via drinking water. Thus systemic infection apparently results from infection of the ileal Peyers patches; a small percentage of the animals develop a concurrent upper respiratory tract infection that may be responsible for the development of carrier states after enteric infection.", "contents": "Involvement of the upper respiratory tract in orally induced salmonellosis in mice. The possible involvement of the tissues of the head and neck during the early pathogenesis of orally or intragastrically induced murine salmonellosis was examined. Viable counts of Salmonella in the local lymphoid tissues and draining nodes indicated that the ileal Peyer's patches were primarily involved in the development of a subsequent enteric fever, irrespective of the method of oral challenge. Contamination of drinking water with a high concentration of salmonellae produced infection of the ileal Peyers patches and the eventual development of systemic disease. The cervical lymph nodes were sporadically involved early in the infection, but this involvement soon became general, with large numbers of salmonellae in the cervical nodes by 36 hr. A lower concentration of salmonellae in drinking water led to primary involvement of the ileal Peyers patches; upper respiratory tract involvement was detected much later in the infection. Intragastrically invected mice exhibited the same low-level infection of the cervical lymph nodes as that in mice infected via drinking water. Thus systemic infection apparently results from infection of the ileal Peyers patches; a small percentage of the animals develop a concurrent upper respiratory tract infection that may be responsible for the development of carrier states after enteric infection."}
{"id": "PMID:1092771", "title": "Intracellular bactericidal activity of leukocytes in whole blood for the diagnosis of chronic granulomatous disease of childhood.", "content": "A simplified method for the assay fo intracellular bactericidal activity of leukocytes in whole blood was developed. Providing that serum-resistant organisms are studied, the method can be used with gram-negative baccilli as well as with Staphylococcus aureus. The abnormalities characteristic of patients with chronic granulomatous disease of childhood and their mothers can be readily detected by this technique. By this means an abnormality in intracellular killing of Pseudomonas cepacia in a patient with chronic granulomatous disease and in his heterozygotic mother was found.", "contents": "Intracellular bactericidal activity of leukocytes in whole blood for the diagnosis of chronic granulomatous disease of childhood. A simplified method for the assay fo intracellular bactericidal activity of leukocytes in whole blood was developed. Providing that serum-resistant organisms are studied, the method can be used with gram-negative baccilli as well as with Staphylococcus aureus. The abnormalities characteristic of patients with chronic granulomatous disease of childhood and their mothers can be readily detected by this technique. By this means an abnormality in intracellular killing of Pseudomonas cepacia in a patient with chronic granulomatous disease and in his heterozygotic mother was found."}
{"id": "PMID:1092772", "title": "Transient bacteremia associated with percutaneous liver biopsy.", "content": "Transient bacteremia associated with percutaneous liver biopsy was studied by pour-plate blood cultures, which were obtained immediately before and after the procedure and 5, 10, 15, and 30 min later in 89 patients. Part of the liver tissue was also cultured in all patients. Histological diagnoses included hepatitis, cirrhosis, cholangitis, fatty liver, granulomata, metastatic liver disease, lymphoma, and miscellaneous disorders. All blood cultures obtained before liver biopsy were sterile. Bacteremia was demonstrable in 12 patients (13.48%). In most of these patients, blood cultures were positive for as long as 15 min after liver biopsy; all cultures were negative at 30 min. Among the bacteria associated with 12 episodes of bacteremia were Escherichia coli, Klebsiella, Bacteroides, enterococci, diphtheroids, Staphylococcus aureus, alpha-hemolytic Streptococcus, and Streptococcus pneumoniae. The patients with positive liver biopsies had a higher incidence of bacteremia (83.3%) than did the patients whose liver biopsies were sterile (8.r%); this difference is stastically significant (P smaller than 0.01). Thus, liver biopsy can be associated with transient bactermia.", "contents": "Transient bacteremia associated with percutaneous liver biopsy. Transient bacteremia associated with percutaneous liver biopsy was studied by pour-plate blood cultures, which were obtained immediately before and after the procedure and 5, 10, 15, and 30 min later in 89 patients. Part of the liver tissue was also cultured in all patients. Histological diagnoses included hepatitis, cirrhosis, cholangitis, fatty liver, granulomata, metastatic liver disease, lymphoma, and miscellaneous disorders. All blood cultures obtained before liver biopsy were sterile. Bacteremia was demonstrable in 12 patients (13.48%). In most of these patients, blood cultures were positive for as long as 15 min after liver biopsy; all cultures were negative at 30 min. Among the bacteria associated with 12 episodes of bacteremia were Escherichia coli, Klebsiella, Bacteroides, enterococci, diphtheroids, Staphylococcus aureus, alpha-hemolytic Streptococcus, and Streptococcus pneumoniae. The patients with positive liver biopsies had a higher incidence of bacteremia (83.3%) than did the patients whose liver biopsies were sterile (8.r%); this difference is stastically significant (P smaller than 0.01). Thus, liver biopsy can be associated with transient bactermia."}
{"id": "PMID:1092773", "title": "Nosocomial infection caused by gentamicin-resistant, streptomycin-sensitive Klebsiella.", "content": "Resistance to gentamicin increased abruptly among nosocomial isolates of Klebsiella and Enterobacter at the New York Veterans Administration Hospital in 1973 and 1974. A prospective clinical survey revealed a greater incidence of true infection caused by Klebsiella than by Enterobacter. The initial site of implantation was usually the urinary tract. Multiple serotypes were involved, and one of these was found in rectal swabs of patients treated with antibiotics. Gentamicin resistance declined rapidly after the use of this antibiotic was restricted and increased several months after restrictions were removed. Gentamicin-resistant strains of Klebsiella were uniformly sensitive to amikacin; 75% were sensitive to polymyxin B and 70%  to streptomycin. Antibiotic sensitivity among gram-negative pathogens might be preserved by a program in which the use of highly effective agents is periodically rotated.", "contents": "Nosocomial infection caused by gentamicin-resistant, streptomycin-sensitive Klebsiella. Resistance to gentamicin increased abruptly among nosocomial isolates of Klebsiella and Enterobacter at the New York Veterans Administration Hospital in 1973 and 1974. A prospective clinical survey revealed a greater incidence of true infection caused by Klebsiella than by Enterobacter. The initial site of implantation was usually the urinary tract. Multiple serotypes were involved, and one of these was found in rectal swabs of patients treated with antibiotics. Gentamicin resistance declined rapidly after the use of this antibiotic was restricted and increased several months after restrictions were removed. Gentamicin-resistant strains of Klebsiella were uniformly sensitive to amikacin; 75% were sensitive to polymyxin B and 70%  to streptomycin. Antibiotic sensitivity among gram-negative pathogens might be preserved by a program in which the use of highly effective agents is periodically rotated."}
{"id": "PMID:1092774", "title": "Antibody-coated bacteria in the urine of obstetrical patients with acute pyelonephritis.", "content": "The direct immunofluorescence method for the detection of antibody-coated bacteria in urine sediments was used to test urine samples from obstetrical patients with the clinical diagnosis of acute pyelonephritis or cystitis. Antibody-coated bacteria were present in the urine from 12 of 15 patients with acute pyelonephritis, but they were not observed in the urine from 13 patients with cystitis. The clases of antibody coating the bacteria were IgG, IgA, and, in some cases, IgM. A correlation between a high titer of antibody in serum and the presence of antibody-coated bacteria in the urine was noted. These results confirm that the immunofluorescence test can be useful, as previously reported, in distinguishing infection of the kidney from infection of the bladder.", "contents": "Antibody-coated bacteria in the urine of obstetrical patients with acute pyelonephritis. The direct immunofluorescence method for the detection of antibody-coated bacteria in urine sediments was used to test urine samples from obstetrical patients with the clinical diagnosis of acute pyelonephritis or cystitis. Antibody-coated bacteria were present in the urine from 12 of 15 patients with acute pyelonephritis, but they were not observed in the urine from 13 patients with cystitis. The clases of antibody coating the bacteria were IgG, IgA, and, in some cases, IgM. A correlation between a high titer of antibody in serum and the presence of antibody-coated bacteria in the urine was noted. These results confirm that the immunofluorescence test can be useful, as previously reported, in distinguishing infection of the kidney from infection of the bladder."}
{"id": "PMID:1092775", "title": "Meningitis caused by Streptococcus in adults.", "content": "Meningitis caused by Streptococcus is uncommon in adults. Otitis media, mastoiditis, sinusitis, or trauma has antedated most cases reported in the past. Data on 10 recent cases suggest changes in the current pathogenesis of streptococcal meningitis in adults and emphasize the importance of accurate streptococcal speciation. Endocarditis was present in five cases; trauma played a role in two others. Brain abscess, corticosteroids, alcoholic cirrhosis, and peritonitis secondary to chronic peritoneal dialysis were etiologic factors in five patients. Otitis media, mastoiditis, and sinusitis were conspicuously absent. Streptococcus agalctiae accounted for purulent meningitis in two postmenopausal women. Both strains of group B Streptococcus were bacitracin-sensitive and were thus mislabeled group A. Recognition of Streptococcus bovis spared two patients unnecessary aminoglycoside adminstration. Nine of the 10 patients survived, including a women with prosthetic mitral valve endocarditis and a man with a ruptured brain abscess. Differentiation of these streptococci from Streptococcus pneumoniae was seldon possible on the basis of the spinal fluid gram stain alone.", "contents": "Meningitis caused by Streptococcus in adults. Meningitis caused by Streptococcus is uncommon in adults. Otitis media, mastoiditis, sinusitis, or trauma has antedated most cases reported in the past. Data on 10 recent cases suggest changes in the current pathogenesis of streptococcal meningitis in adults and emphasize the importance of accurate streptococcal speciation. Endocarditis was present in five cases; trauma played a role in two others. Brain abscess, corticosteroids, alcoholic cirrhosis, and peritonitis secondary to chronic peritoneal dialysis were etiologic factors in five patients. Otitis media, mastoiditis, and sinusitis were conspicuously absent. Streptococcus agalctiae accounted for purulent meningitis in two postmenopausal women. Both strains of group B Streptococcus were bacitracin-sensitive and were thus mislabeled group A. Recognition of Streptococcus bovis spared two patients unnecessary aminoglycoside adminstration. Nine of the 10 patients survived, including a women with prosthetic mitral valve endocarditis and a man with a ruptured brain abscess. Differentiation of these streptococci from Streptococcus pneumoniae was seldon possible on the basis of the spinal fluid gram stain alone."}
{"id": "PMID:1092781", "title": "Otology in the Bible and Talmud.", "content": "The present paper is an English translation of the chapter of Otology in Preuss's classic book Biblisch-Talmudische Medizin. Described in the Talmud are a variety of anatomical ear abnormalities such as double ear, pierced ears, small ears, cut off ears, and pendulous ears. A slave whose ear was wounded had to be freed. Compensation for injury to the ear and to hearing is clearly noted. Remedies for earache are mentioned. The legal status of a deaf-mute, and the relationship of deafness to muteness are also discussed in the Talmud.", "contents": "Otology in the Bible and Talmud. The present paper is an English translation of the chapter of Otology in Preuss's classic book Biblisch-Talmudische Medizin. Described in the Talmud are a variety of anatomical ear abnormalities such as double ear, pierced ears, small ears, cut off ears, and pendulous ears. A slave whose ear was wounded had to be freed. Compensation for injury to the ear and to hearing is clearly noted. Remedies for earache are mentioned. The legal status of a deaf-mute, and the relationship of deafness to muteness are also discussed in the Talmud."}
{"id": "PMID:1092784", "title": "Determination of bile acid pool size in man: a simplified method with advantages of increases precision, shortened analysis time, and decreased isotope exposure.", "content": "A simplified isotope dilution method for measurement of the bile acid pool size in normal subjects is described and compared with the traditional method of Lindstedt (Acta Physiol. Scand. 40: 1-9, 1957). Advantages of this simplified method include a four- to eightfold reduction of isotope dose, facilitation of analytical procedures, and a reduction in the required number of duodenal intubations. In 15 human subjects who had two separate estimates of pool size by this method, precision averaged 2.6 percent. In 16 comparisons, pool size measured by this method averaged 13.7 percent higher than simultaneous estimates by the Lindstedt method. Factors affecting accuracy (as opposed to precision) in both methods are discussed.", "contents": "Determination of bile acid pool size in man: a simplified method with advantages of increases precision, shortened analysis time, and decreased isotope exposure. A simplified isotope dilution method for measurement of the bile acid pool size in normal subjects is described and compared with the traditional method of Lindstedt (Acta Physiol. Scand. 40: 1-9, 1957). Advantages of this simplified method include a four- to eightfold reduction of isotope dose, facilitation of analytical procedures, and a reduction in the required number of duodenal intubations. In 15 human subjects who had two separate estimates of pool size by this method, precision averaged 2.6 percent. In 16 comparisons, pool size measured by this method averaged 13.7 percent higher than simultaneous estimates by the Lindstedt method. Factors affecting accuracy (as opposed to precision) in both methods are discussed."}
{"id": "PMID:1092785", "title": "Lyophilized 7 alpha-hydroxysteroid dehydrogenase: a stable enzyme preparation for routine bile acid analysis.", "content": "Preparations of 7 alpha-hydroxysteroid dehydrogenase (7 alpha-HSDH) from Escherichia coli strain 23 can be frozen and thawed without significant loss of activity. 7 alpha-HSDH may then be lyophilized into powder form, which is stable for more than 6 months (3% loss of activity). The lyophilized 7 alpha-HSDH preparation has the additional advantage over previously described preparations of a low and stable fluorescence background when applied to the fluorometric estimation of bile acids, especially in combination with thin-layer chromatography. Analysis of duodenal aspirates from 18 normal subjects gave bile acid ratios identical with those reported earlier and obtained by using gas-liquid chromatography. A significant difference in the glycine: taurine ratio between males and females was observed.", "contents": "Lyophilized 7 alpha-hydroxysteroid dehydrogenase: a stable enzyme preparation for routine bile acid analysis. Preparations of 7 alpha-hydroxysteroid dehydrogenase (7 alpha-HSDH) from Escherichia coli strain 23 can be frozen and thawed without significant loss of activity. 7 alpha-HSDH may then be lyophilized into powder form, which is stable for more than 6 months (3% loss of activity). The lyophilized 7 alpha-HSDH preparation has the additional advantage over previously described preparations of a low and stable fluorescence background when applied to the fluorometric estimation of bile acids, especially in combination with thin-layer chromatography. Analysis of duodenal aspirates from 18 normal subjects gave bile acid ratios identical with those reported earlier and obtained by using gas-liquid chromatography. A significant difference in the glycine: taurine ratio between males and females was observed."}
{"id": "PMID:1092788", "title": "Genetical control of B-cell responses. III. Requirement for functional mitogenicity of the antigen in thymus-independent specific responses.", "content": "Spleen cells from C3H/HeJ mice fail to respond with polyclonal antibody synthesis to mitogenic concentrations of lipopolysaccharide (LPS) which are optimal for activating spleen cells from a high-responder strain (B10.5M). This unresponsiveness is selective for LPS, since C3H/HeJ cells respond as normals to another B-cell mitogen, purified protein derivative of tuberculin. Spleen cells from low-responder mice also fail to mount a specific anti-NNP plaque-forming cell (PFC) response, when challenged in vitro by NNP-LPS. However, C3H/HeJ cells develop normal responses to another thymus-independent hapten conjugate, DNP-AECM-Ficoll. C3H/HeJ mice fail to mount a specific anti-LPS antibody response, when challenged in vivo with doses of soluble LPS which are fully immunogenic for the high-responder strain. However, C3H/HeJ mice develop normal direct and indirect PFC responses to LPS, when challenged with a thymus-dependent form of the immunogen. These results are interpreted as indicating as absolute requirement for functional mitogenicity of the antigen, in the induction of specific thymus-independent antibody responses.", "contents": "Genetical control of B-cell responses. III. Requirement for functional mitogenicity of the antigen in thymus-independent specific responses. Spleen cells from C3H/HeJ mice fail to respond with polyclonal antibody synthesis to mitogenic concentrations of lipopolysaccharide (LPS) which are optimal for activating spleen cells from a high-responder strain (B10.5M). This unresponsiveness is selective for LPS, since C3H/HeJ cells respond as normals to another B-cell mitogen, purified protein derivative of tuberculin. Spleen cells from low-responder mice also fail to mount a specific anti-NNP plaque-forming cell (PFC) response, when challenged in vitro by NNP-LPS. However, C3H/HeJ cells develop normal responses to another thymus-independent hapten conjugate, DNP-AECM-Ficoll. C3H/HeJ mice fail to mount a specific anti-LPS antibody response, when challenged in vivo with doses of soluble LPS which are fully immunogenic for the high-responder strain. However, C3H/HeJ mice develop normal direct and indirect PFC responses to LPS, when challenged with a thymus-dependent form of the immunogen. These results are interpreted as indicating as absolute requirement for functional mitogenicity of the antigen, in the induction of specific thymus-independent antibody responses."}
{"id": "PMID:1092789", "title": "Immunologic injury of cultured cells infected with measles virus. I. role of IfG antibody and the alternative complement pathway.", "content": "In these studies, a number of human cell lines including epithelial, neural, glial, and lymphoid cells infected with several strains of measles virus were found to be lysed upon incubation with fresh sera from humans containing antibody measles virus. In all instances, the cytolytic event was mediated by alternative complement (C) pathway without a significant contribution from classical pathway. In contrast, isolated measles virus in conjunction with antibody was found to selectively activate the classical C pathway. Measles antibodies of the IgG class, but not the IgA class, possessed cytolytic potential against cells infected with measles virus. Human IgG antibodies could directly activate the alternative C pathway. No defect was found in cytolytic measles antibody in sera or cerebrospinal fluid from patients with subacute sclerosing panencephalitis, nor was the alternative C pathway impaired in sera from these patients. Sera from newborn humans exhibited a functional alternative C pathway.", "contents": "Immunologic injury of cultured cells infected with measles virus. I. role of IfG antibody and the alternative complement pathway. In these studies, a number of human cell lines including epithelial, neural, glial, and lymphoid cells infected with several strains of measles virus were found to be lysed upon incubation with fresh sera from humans containing antibody measles virus. In all instances, the cytolytic event was mediated by alternative complement (C) pathway without a significant contribution from classical pathway. In contrast, isolated measles virus in conjunction with antibody was found to selectively activate the classical C pathway. Measles antibodies of the IgG class, but not the IgA class, possessed cytolytic potential against cells infected with measles virus. Human IgG antibodies could directly activate the alternative C pathway. No defect was found in cytolytic measles antibody in sera or cerebrospinal fluid from patients with subacute sclerosing panencephalitis, nor was the alternative C pathway impaired in sera from these patients. Sera from newborn humans exhibited a functional alternative C pathway."}
{"id": "PMID:1092790", "title": "Study of the cells proliferating in parent versus F hybrid mixed lymphocyte culture.", "content": "Caryotypic analysis of the cells dividing in mouse parent-hybrid MLC showed an F1 hybrid cell proliferation, which varied depending upon the source of lymphoid cells used: strong in spleen MLC (sometimes equal to that of the parental cells), less marked in lymph node cell MLC, and most often absent in MLC between cortisone-resistant (CR) thymocytes. MLC between parental spleen cells and F1 CR thymocytes showed, however, that in certain conditions of culture F thymocytes can also proliferate. Using parental or F1 spleen cells lacking T lymphocytes, it was found that F1 cell proliferation is entirely dependent upon the presence of parental T cells, but does not require the presence of T lymphocytes among the F1 cells. Immunofluorescence analysis of the blasts observed in one-way MLC showed that about 70% of the parental blasts were T blasts, and 25%B blasts (containing a high proportion of plasmablasts); among the F1 blasts, there was also the same percentage of B blasts and plasmablasts, but many of the T blasts bore only small amounts of T-cell antigen (MTLA), and there was also about 20%of unstained blasts, possibly T blasts bearing MTLA in amounts undetectable by immunofluorescence. The possibility is discussed that the F1 responding T cells belong to a subpopulation performing a suppressive function; MLC lacking F1 T cells showed increased [3H] thymidine incorporation. The proliferation and differentiation of parental and F1 B cells may result mainly from an unspecific, \"polyclonal\" triggering.", "contents": "Study of the cells proliferating in parent versus F hybrid mixed lymphocyte culture. Caryotypic analysis of the cells dividing in mouse parent-hybrid MLC showed an F1 hybrid cell proliferation, which varied depending upon the source of lymphoid cells used: strong in spleen MLC (sometimes equal to that of the parental cells), less marked in lymph node cell MLC, and most often absent in MLC between cortisone-resistant (CR) thymocytes. MLC between parental spleen cells and F1 CR thymocytes showed, however, that in certain conditions of culture F thymocytes can also proliferate. Using parental or F1 spleen cells lacking T lymphocytes, it was found that F1 cell proliferation is entirely dependent upon the presence of parental T cells, but does not require the presence of T lymphocytes among the F1 cells. Immunofluorescence analysis of the blasts observed in one-way MLC showed that about 70% of the parental blasts were T blasts, and 25%B blasts (containing a high proportion of plasmablasts); among the F1 blasts, there was also the same percentage of B blasts and plasmablasts, but many of the T blasts bore only small amounts of T-cell antigen (MTLA), and there was also about 20%of unstained blasts, possibly T blasts bearing MTLA in amounts undetectable by immunofluorescence. The possibility is discussed that the F1 responding T cells belong to a subpopulation performing a suppressive function; MLC lacking F1 T cells showed increased [3H] thymidine incorporation. The proliferation and differentiation of parental and F1 B cells may result mainly from an unspecific, \"polyclonal\" triggering."}
{"id": "PMID:1092791", "title": "Effects on C-reactive protein on the lymphoid system. I. Binding to thymus-dependent lymphocytes and alteration of their functions.", "content": "C-reactive protein (CRP) is an acute phase protein which shares with the immunoglobulins the ability to induce precipitation and agglutination reactions and activate the complement system. We report here that purified human CRP binds selectively to human T lymphocytes, inhibits their ability to form spontaneous rosettes with sheep erythrocytes and inhibits their response to allogeneic cells in mixed lymphocyte culture reactions; it fails to inhibit phytohemagglutinin- or concanavalin-A-induced mitogenesis. CRP does not bind to human B lymphocytes, nor does it alter the following B-cell functions: binding to activated complement components or the Fc portion of immunoglobulins, mediation of antibody-dependent cytotoxicity reactions or the ability of allogeneic cells to stimulate a mixed lymphocyte culture reaction. Human CRP shows similar selective binding with murine T lymphocytes. It therefore seems that binding of CRP is a property of T lymphocytes or a subpopulation thereof, and can result in modulation of certain of the T-cell functional characteristics in vitro. We suggest that CRP may play a role in modulating T-cell functions during the inflammatory state.", "contents": "Effects on C-reactive protein on the lymphoid system. I. Binding to thymus-dependent lymphocytes and alteration of their functions. C-reactive protein (CRP) is an acute phase protein which shares with the immunoglobulins the ability to induce precipitation and agglutination reactions and activate the complement system. We report here that purified human CRP binds selectively to human T lymphocytes, inhibits their ability to form spontaneous rosettes with sheep erythrocytes and inhibits their response to allogeneic cells in mixed lymphocyte culture reactions; it fails to inhibit phytohemagglutinin- or concanavalin-A-induced mitogenesis. CRP does not bind to human B lymphocytes, nor does it alter the following B-cell functions: binding to activated complement components or the Fc portion of immunoglobulins, mediation of antibody-dependent cytotoxicity reactions or the ability of allogeneic cells to stimulate a mixed lymphocyte culture reaction. Human CRP shows similar selective binding with murine T lymphocytes. It therefore seems that binding of CRP is a property of T lymphocytes or a subpopulation thereof, and can result in modulation of certain of the T-cell functional characteristics in vitro. We suggest that CRP may play a role in modulating T-cell functions during the inflammatory state."}
{"id": "PMID:1092792", "title": "Elevated ribonuclease activity in the thymus and white blood cells of genetically cancer prone mice.", "content": "Ribonuclease activity in cell-free thymus homogenates was elevated for five strains of mice genetically predisposed toward leukemia or reticulum cell neoplasms (AKR, C58, PL, RF, and SJL). Such increased activity was directed against polyuridylic acid and was observed in 8-wk old mice, well before the onset of neoplastic transformation. Similarly, white blood cell ribonuclease activity was elevated in mice of the strains AKR, C2H/He, PL and RF. Statistical analysis indicated that such elevated activity in these strains related to their high incidence of spontaneous neoplastic disease. Elevated ribonuclease activity thus represents a new biochemical marker relating to the genetic propensity of some strains of mice to die prematurely of spontaneous neoplasia.", "contents": "Elevated ribonuclease activity in the thymus and white blood cells of genetically cancer prone mice. Ribonuclease activity in cell-free thymus homogenates was elevated for five strains of mice genetically predisposed toward leukemia or reticulum cell neoplasms (AKR, C58, PL, RF, and SJL). Such increased activity was directed against polyuridylic acid and was observed in 8-wk old mice, well before the onset of neoplastic transformation. Similarly, white blood cell ribonuclease activity was elevated in mice of the strains AKR, C2H/He, PL and RF. Statistical analysis indicated that such elevated activity in these strains related to their high incidence of spontaneous neoplastic disease. Elevated ribonuclease activity thus represents a new biochemical marker relating to the genetic propensity of some strains of mice to die prematurely of spontaneous neoplasia."}
{"id": "PMID:1092793", "title": "Proliferation and colony-forming ability of peritoneal exudate cells in liquid culture.", "content": "Peritoneal exudate cells, obtained from mice injected with thioglycollate medium and cultured in medium containing L-cell-conditioned medium, will proliferate in an exponential fashion for 18 days with a doubling time of 68 h. After a 2 h pulse of tritiated thymidine, labeled adherent cells increased to a maximum of 22-34% during the 1st and 2nd wk of culture. Increasing the cell concentration from 2 times 10-3 to 2 times 10-5 cells/culture reduced exponential growth to 10 days and the doubling time was increased to 81.6 h. Under these culture conditions, peritoneal exudate cells were shown to form colonies on the surface of culture dishes when plated at low density. The cells within the colony were shown to be macrophages using yeast and antibody-coated sheep erythrocytes as a test for phagocytic function. The plating efficiolonies arose from a single precursor cell. The adherent cell population contains the colony-forming precursors. These precursors can be stimulated to form colonies for at least 2 wk by the addition of conditioned medium to cultures at various times after plating. While very few colony-forming cells could be demonstrated in the unstimulated peritoneal lavage, their numbers begin to increase in the exudate 4 h after injection of thioglycollate medium and reach a maximum by day 3 and then decrease. Isolated colonies may be useful in studying the function of macrophages.", "contents": "Proliferation and colony-forming ability of peritoneal exudate cells in liquid culture. Peritoneal exudate cells, obtained from mice injected with thioglycollate medium and cultured in medium containing L-cell-conditioned medium, will proliferate in an exponential fashion for 18 days with a doubling time of 68 h. After a 2 h pulse of tritiated thymidine, labeled adherent cells increased to a maximum of 22-34% during the 1st and 2nd wk of culture. Increasing the cell concentration from 2 times 10-3 to 2 times 10-5 cells/culture reduced exponential growth to 10 days and the doubling time was increased to 81.6 h. Under these culture conditions, peritoneal exudate cells were shown to form colonies on the surface of culture dishes when plated at low density. The cells within the colony were shown to be macrophages using yeast and antibody-coated sheep erythrocytes as a test for phagocytic function. The plating efficiolonies arose from a single precursor cell. The adherent cell population contains the colony-forming precursors. These precursors can be stimulated to form colonies for at least 2 wk by the addition of conditioned medium to cultures at various times after plating. While very few colony-forming cells could be demonstrated in the unstimulated peritoneal lavage, their numbers begin to increase in the exudate 4 h after injection of thioglycollate medium and reach a maximum by day 3 and then decrease. Isolated colonies may be useful in studying the function of macrophages."}
{"id": "PMID:1092794", "title": "T-cell migration into allografts.", "content": "The ability of T and B lymphocytes to migrate into skin allografts undergoing rejection was studied in mice. Spleen cells from CBA/J mice sensitized to transplantation antigens of A/J or C57BL/6 mice were separated on immunabsorbent columns into purified populations of T and B cells, labeled in vitro with 3H-uridine and injected intravenously into CBA/J mice with 7-day old skin iso and allografts (A/J or C57BL/6). The mice were sacrificed 24 h later and studied by autoradiography. After transfer of either unfractionated spleen cells or T cells, large numbers of labeled cells were found in the cellular infiltrate of allografts, whereas extremely few were seen in isografts. In contrast, after transfer of B cells, almost no labeled cells were detected either in the allografts or the isografts, although they, like T cells, homed normally to lymphoid tissue.", "contents": "T-cell migration into allografts. The ability of T and B lymphocytes to migrate into skin allografts undergoing rejection was studied in mice. Spleen cells from CBA/J mice sensitized to transplantation antigens of A/J or C57BL/6 mice were separated on immunabsorbent columns into purified populations of T and B cells, labeled in vitro with 3H-uridine and injected intravenously into CBA/J mice with 7-day old skin iso and allografts (A/J or C57BL/6). The mice were sacrificed 24 h later and studied by autoradiography. After transfer of either unfractionated spleen cells or T cells, large numbers of labeled cells were found in the cellular infiltrate of allografts, whereas extremely few were seen in isografts. In contrast, after transfer of B cells, almost no labeled cells were detected either in the allografts or the isografts, although they, like T cells, homed normally to lymphoid tissue."}
{"id": "PMID:1092795", "title": "Rubella cataract in vitro: Sensitive period of the developing human lens.", "content": "The clinically known sensitive period of rubella cataract was studied in vitro by infecting 79 human eye rudiments from embryos aged 4-10 wk with rubella virus. The course of the infection was followed by histological and indirect immunofluorescence methods. Of the rudiments, 12 pairs were in the lens placode or open-lens-vesicle stage, 40 already had closed lens vesicles and in another 27 closed-stage pairs an incision was made in the lens capsule before infection to allow the virus to enter the lens. Uninfected controls differentiated well in vitro for 4-6 wk. The eye rudiments infected in the open-lens-vesicle stage showed lens fiber destruction and viral antigens within the lens. No damage or viral antigens were detected in rudiments infected in the closed stage unless the lens capsule was incisedmwhen this was done, however, fiber damage ensued and viral antigens appeared. The lens capsule was concluded to form a protective barrier around the sensirive fibers at the time of closure of the lens vesicle, confirming the earlier hypothesis and clinical findings.", "contents": "Rubella cataract in vitro: Sensitive period of the developing human lens. The clinically known sensitive period of rubella cataract was studied in vitro by infecting 79 human eye rudiments from embryos aged 4-10 wk with rubella virus. The course of the infection was followed by histological and indirect immunofluorescence methods. Of the rudiments, 12 pairs were in the lens placode or open-lens-vesicle stage, 40 already had closed lens vesicles and in another 27 closed-stage pairs an incision was made in the lens capsule before infection to allow the virus to enter the lens. Uninfected controls differentiated well in vitro for 4-6 wk. The eye rudiments infected in the open-lens-vesicle stage showed lens fiber destruction and viral antigens within the lens. No damage or viral antigens were detected in rudiments infected in the closed stage unless the lens capsule was incisedmwhen this was done, however, fiber damage ensued and viral antigens appeared. The lens capsule was concluded to form a protective barrier around the sensirive fibers at the time of closure of the lens vesicle, confirming the earlier hypothesis and clinical findings."}
{"id": "PMID:1092796", "title": "Characterization of the macrophage receptro for complement and demonstration of its functional independence from the receptor for the Fc portion of immunoglobulin G.", "content": "The complement receptor of the macrophage membrane recognizes particle-bound C3b but does not recognize particle-bound C3d. C3-b-coated sheep erythrocytes were bound to macrophages via their C3b receptors, and the preparations were then incubated with either latex particles or opsonized pneumococci (test particles). Macrophages ingested the test particles, but erythrocytes were not ingested; they remained bound to C3b receptors of the macrophage plasma membrane. Thus, a signal initiating ingestion via one type of receptor is not transmitted to all receptors which have the potential to mediate phagocytosis.", "contents": "Characterization of the macrophage receptro for complement and demonstration of its functional independence from the receptor for the Fc portion of immunoglobulin G. The complement receptor of the macrophage membrane recognizes particle-bound C3b but does not recognize particle-bound C3d. C3-b-coated sheep erythrocytes were bound to macrophages via their C3b receptors, and the preparations were then incubated with either latex particles or opsonized pneumococci (test particles). Macrophages ingested the test particles, but erythrocytes were not ingested; they remained bound to C3b receptors of the macrophage plasma membrane. Thus, a signal initiating ingestion via one type of receptor is not transmitted to all receptors which have the potential to mediate phagocytosis."}
{"id": "PMID:1092797", "title": "Clonal dominance. I. Restricted nature of the IgM antibody response to group A streptococcal carbohydrate in mice.", "content": "The IgM antibody response of mice to the streptococcal group A carbohydrate (GAC) was measured. With most strains tested, large amounts of IgM antibody were produced; in AKR mice, over 1% of the total nucleated spleen cells secreted IgM anti-GAC antibody after hyperimmunization. The relative avidity of the antibody was extimated by a modification of the Jerne plaque assay where spleen cells from individual mice were tested against erythrocytes with varying GAC epitope densitymthese studies showed that the earliest, as well as latest, IgM antibodies produced were highly restricted in avidity heterogeneity. No evidence of affinity maturation was seen upon hyperimmunization. These data favor the conclusion that the restricted IgG response seen in mice hyperimmunized to GAC is not the result of affinity driven competition for antigen among precursor cells.", "contents": "Clonal dominance. I. Restricted nature of the IgM antibody response to group A streptococcal carbohydrate in mice. The IgM antibody response of mice to the streptococcal group A carbohydrate (GAC) was measured. With most strains tested, large amounts of IgM antibody were produced; in AKR mice, over 1% of the total nucleated spleen cells secreted IgM anti-GAC antibody after hyperimmunization. The relative avidity of the antibody was extimated by a modification of the Jerne plaque assay where spleen cells from individual mice were tested against erythrocytes with varying GAC epitope densitymthese studies showed that the earliest, as well as latest, IgM antibodies produced were highly restricted in avidity heterogeneity. No evidence of affinity maturation was seen upon hyperimmunization. These data favor the conclusion that the restricted IgG response seen in mice hyperimmunized to GAC is not the result of affinity driven competition for antigen among precursor cells."}
{"id": "PMID:1092798", "title": "Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen.", "content": "Ly alloantigens coded by two unlinked genetic loci (Ly-1 and Ly-2/Ly-3) are expressed on lymphoid cells undergoing thymus-dependent differentiation. Peripheral Thy-1+ cells from C57BL/6 mice can be divided into three subclasses on the basis of differential expression of Ly-1, Ly-2, and Ly-3; about 50% express all three Ly antigens (Ly -123+), about 33% only Ly-1 (Ly-1+), and about 6-8% Ly-2 and Ly-3 (Ly-23+). Cells of the Ly-123+ subclasses are the first peripheral Thy-1+ cells to appear in ontogeny, and are reduced in the periphery shortly after adult thymectomy. In contrast, Ly-1+ and Ly-23+ subclasses appear later in the peripheral tissues than do Ly-123+ cells, and are resistant to the early effects of adult thymectomymperiheral lymphoid populations depleted of Ly-1+ cells and Ly-123+ cells (and thereby enriched for Ly-23+ cells) were incapable of developing significant helper activity to SRBC but generated substantial levels of cytotoxic activity to allogeneic target cells. The same lymphoid populations, depleted of Ly-23+ cells and Ly-123+ cells (and thereby enriched for Ly-1+ cells), produced substantial helper responses but were unable to generate appreciable levels of killer activity. These experiments imply that commitment of T cells to participate exclusively in either helper or cytotoxic function is a differentiative process that takes place before they encounter antigen, and is accompanied by exclusion of different Ly groups, Lu-23 or Ly-1 respectively, from TL+Ly-123+ T-cell precursors. It is yet to be decided whether the TL-phase by Ly-123+ subclass is a transitional form or a separately differentiated subclass with a discrete immunologic function.", "contents": "Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. Ly alloantigens coded by two unlinked genetic loci (Ly-1 and Ly-2/Ly-3) are expressed on lymphoid cells undergoing thymus-dependent differentiation. Peripheral Thy-1+ cells from C57BL/6 mice can be divided into three subclasses on the basis of differential expression of Ly-1, Ly-2, and Ly-3; about 50% express all three Ly antigens (Ly -123+), about 33% only Ly-1 (Ly-1+), and about 6-8% Ly-2 and Ly-3 (Ly-23+). Cells of the Ly-123+ subclasses are the first peripheral Thy-1+ cells to appear in ontogeny, and are reduced in the periphery shortly after adult thymectomy. In contrast, Ly-1+ and Ly-23+ subclasses appear later in the peripheral tissues than do Ly-123+ cells, and are resistant to the early effects of adult thymectomymperiheral lymphoid populations depleted of Ly-1+ cells and Ly-123+ cells (and thereby enriched for Ly-23+ cells) were incapable of developing significant helper activity to SRBC but generated substantial levels of cytotoxic activity to allogeneic target cells. The same lymphoid populations, depleted of Ly-23+ cells and Ly-123+ cells (and thereby enriched for Ly-1+ cells), produced substantial helper responses but were unable to generate appreciable levels of killer activity. These experiments imply that commitment of T cells to participate exclusively in either helper or cytotoxic function is a differentiative process that takes place before they encounter antigen, and is accompanied by exclusion of different Ly groups, Lu-23 or Ly-1 respectively, from TL+Ly-123+ T-cell precursors. It is yet to be decided whether the TL-phase by Ly-123+ subclass is a transitional form or a separately differentiated subclass with a discrete immunologic function."}
{"id": "PMID:1092799", "title": "Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity.", "content": "Lymphocytes from BALB/c or C57B6/6 mice that develop killer activity to alloantigens belong to the numerically small Ly-23 subclass of peripheral T cells, distinguished by selective expression of Ly-23 determinants on their surfaces. The maturation of these cells to killer cells can be amplified by Ly-1+ cells, which do not themselves contribute to the killer cell pool. This amplification was abolished by escluding Ia(\"Beta\")+ cells from the stimulator population during mixed lymphocyte culture (MCL), suggesting that amplification is due to selective recognition of I region antigens by L-1+ cells, a conclusion already drawn from our previous evidence that Ia differences activate Ly-1+ cells but not Ly-23+ cells. These and other experiments indicate that amplification of killer cell production in vitro by Ly-1+ cells is not due to their conversion to Ly-23+ cells during MLC, but to their ability to recognize major histocompatibility complex determinants not recognized by Ly-23+ cells.", "contents": "Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. Lymphocytes from BALB/c or C57B6/6 mice that develop killer activity to alloantigens belong to the numerically small Ly-23 subclass of peripheral T cells, distinguished by selective expression of Ly-23 determinants on their surfaces. The maturation of these cells to killer cells can be amplified by Ly-1+ cells, which do not themselves contribute to the killer cell pool. This amplification was abolished by escluding Ia(\"Beta\")+ cells from the stimulator population during mixed lymphocyte culture (MCL), suggesting that amplification is due to selective recognition of I region antigens by L-1+ cells, a conclusion already drawn from our previous evidence that Ia differences activate Ly-1+ cells but not Ly-23+ cells. These and other experiments indicate that amplification of killer cell production in vitro by Ly-1+ cells is not due to their conversion to Ly-23+ cells during MLC, but to their ability to recognize major histocompatibility complex determinants not recognized by Ly-23+ cells."}
{"id": "PMID:1092800", "title": "Office bacteriology in children.", "content": "Office bacteriological procedures can be a valuable part of a physician's diagnostic armamentarium when utilized within the framework of sound clinical judgment. This is particularily true in children, where infectious diseases represent the major portion of illness. The techniques are simple and inexpensive. The expertise necessary is rapidly and easily acquired. The results can be gratifying and serve as a stimulant for greater involvement in this technique.", "contents": "Office bacteriology in children. Office bacteriological procedures can be a valuable part of a physician's diagnostic armamentarium when utilized within the framework of sound clinical judgment. This is particularily true in children, where infectious diseases represent the major portion of illness. The techniques are simple and inexpensive. The expertise necessary is rapidly and easily acquired. The results can be gratifying and serve as a stimulant for greater involvement in this technique."}
{"id": "PMID:1092801", "title": "Recent advances in diagnosis and management of croup.", "content": "Croup is a syndrome of inspiratory stridor, cough, and hoarseness, due to varying degrees of laryngeal obstruction. It is a viral disease and must be differentiated from epiglottitis. In addition to a careful clinical assessment, neck films are the most valuable diagnostic tool in differentiating these two. The principle modes of therapy for croup include provision of adequate hydration, ensuring maximum available humidification, sedation, and administration of intermittent positive pressure breathing (IPPB) with nebulized racemic epinephrine. The latter mode of therapy will provide symptomatic relief and may eliminate the need for hospitalization and tracheostomy. Steroids offer little benefit in treating this disease, and antibiotics offer none.", "contents": "Recent advances in diagnosis and management of croup. Croup is a syndrome of inspiratory stridor, cough, and hoarseness, due to varying degrees of laryngeal obstruction. It is a viral disease and must be differentiated from epiglottitis. In addition to a careful clinical assessment, neck films are the most valuable diagnostic tool in differentiating these two. The principle modes of therapy for croup include provision of adequate hydration, ensuring maximum available humidification, sedation, and administration of intermittent positive pressure breathing (IPPB) with nebulized racemic epinephrine. The latter mode of therapy will provide symptomatic relief and may eliminate the need for hospitalization and tracheostomy. Steroids offer little benefit in treating this disease, and antibiotics offer none."}
{"id": "PMID:1092802", "title": "The effects of castration and androgen replacement on song, courtship, and aggression in zebra finches (Poephila guttata).", "content": "Castration of adult male zebra finches (Poephila guttata, Estrildidae) reduces their singing rate and the tempo of song, but castrates continue to sing song identical in form to preoperative song. Injection or implantation of testosterone propionate (TP) but not of vehicle alone reverses the changes produced by castration. Castration or partial castration also reduces the frequency of courtship, copulation, and aggression. Androgen (TP) replacement reverses these changes, but control injections do not. The persistence of song after castration contrasts with the abolition of song by castration in other birds, and this may be related to the natural history of zebra finches.", "contents": "The effects of castration and androgen replacement on song, courtship, and aggression in zebra finches (Poephila guttata). Castration of adult male zebra finches (Poephila guttata, Estrildidae) reduces their singing rate and the tempo of song, but castrates continue to sing song identical in form to preoperative song. Injection or implantation of testosterone propionate (TP) but not of vehicle alone reverses the changes produced by castration. Castration or partial castration also reduces the frequency of courtship, copulation, and aggression. Androgen (TP) replacement reverses these changes, but control injections do not. The persistence of song after castration contrasts with the abolition of song by castration in other birds, and this may be related to the natural history of zebra finches."}
{"id": "PMID:1092803", "title": "A method for the chemical isolation of individual muscle fibers and its application to a study of the effect of denervation on the number of nuclei per muscle fiber.", "content": "A method is described for isolating intact, individual skeletal muscle fibers from glutaraldehyde fixed muscle. This method was conceived to climinate the many limitations of determining muscle nuclear numbers in histological cross section. Glutaraldehyde fixed fibers are isolated by dissection while in a solution of low concentration guanidine in a borate buffer at high pH. Electron miscroscopy demonstrates that single fibers, isolated in this manner, are free of their microvasculature and connective tissue. Their basal laminas and the structures within them, including their satellite cells, are preserved. This method is employed to determine whether changes in nuclear numbers occur within the basal lamina of individual muscle cells from 1 to 28 days following denervation of mouse gastrocnemius muscle. The total number of nuclei located within the basal lamina of individual muscle fibers (i.e. muscle and satellite cell nuclei) does not change after denervation. This rules out the possibility that additional nuclei are arising from an influx of cells outside the basal lamina or by mitotic division of nuclei within the basal lamina. However, the possibility of a change in the ratio of satellite cell nuclei, to muscle cell nuclei, is not eliminated. Other possible applications of this isolation method are discussed.", "contents": "A method for the chemical isolation of individual muscle fibers and its application to a study of the effect of denervation on the number of nuclei per muscle fiber. A method is described for isolating intact, individual skeletal muscle fibers from glutaraldehyde fixed muscle. This method was conceived to climinate the many limitations of determining muscle nuclear numbers in histological cross section. Glutaraldehyde fixed fibers are isolated by dissection while in a solution of low concentration guanidine in a borate buffer at high pH. Electron miscroscopy demonstrates that single fibers, isolated in this manner, are free of their microvasculature and connective tissue. Their basal laminas and the structures within them, including their satellite cells, are preserved. This method is employed to determine whether changes in nuclear numbers occur within the basal lamina of individual muscle cells from 1 to 28 days following denervation of mouse gastrocnemius muscle. The total number of nuclei located within the basal lamina of individual muscle fibers (i.e. muscle and satellite cell nuclei) does not change after denervation. This rules out the possibility that additional nuclei are arising from an influx of cells outside the basal lamina or by mitotic division of nuclei within the basal lamina. However, the possibility of a change in the ratio of satellite cell nuclei, to muscle cell nuclei, is not eliminated. Other possible applications of this isolation method are discussed."}
{"id": "PMID:1092804", "title": "The immune response of the teleost, Tilapia mossambica to soluble and cellular antigens.", "content": "The immune response of Tilapia mossambica to bovine serum albumin (BSA) and sheep red blood cells (SRBC) was characterized in detail in terms of the appearance of hemolysin plaque-forming cells and circulating antibodies at 30 degrees C. Plaque-forming cells (PFC) were detected in the spleen, head-kidney and thymus of immunized fish and the maximum number was observed in these organs on the fifth day after immunization with SRBC. Peak circulating antibody response occurred on day 8 for SRBC and on day 11 for BSA. Following the second injection of the same antigen, a specific anamnestic response was observed with increased production of PFC and serum antibody. No cross reactivity was found when anti-SRBC antibody was tested with rat erythrocytes. Tests with 2-mercaptoethanol showed that all of the agglutinating antibody produced after both the first and second injection was mercaptoethanol sensitive. Analysis of histological and smear preparations revealed that there were consistent cellular changes occurring in the spleen as well as the head-kidney due to immunization.", "contents": "The immune response of the teleost, Tilapia mossambica to soluble and cellular antigens. The immune response of Tilapia mossambica to bovine serum albumin (BSA) and sheep red blood cells (SRBC) was characterized in detail in terms of the appearance of hemolysin plaque-forming cells and circulating antibodies at 30 degrees C. Plaque-forming cells (PFC) were detected in the spleen, head-kidney and thymus of immunized fish and the maximum number was observed in these organs on the fifth day after immunization with SRBC. Peak circulating antibody response occurred on day 8 for SRBC and on day 11 for BSA. Following the second injection of the same antigen, a specific anamnestic response was observed with increased production of PFC and serum antibody. No cross reactivity was found when anti-SRBC antibody was tested with rat erythrocytes. Tests with 2-mercaptoethanol showed that all of the agglutinating antibody produced after both the first and second injection was mercaptoethanol sensitive. Analysis of histological and smear preparations revealed that there were consistent cellular changes occurring in the spleen as well as the head-kidney due to immunization."}
{"id": "PMID:1092805", "title": "Induction of nuclear breakdown and meiosis in Spisula solidissima oocytes by calcium ionophore.", "content": "Induction of germinal vesicle breakdown and reinitiation of the meiotic process resulted following exposure of immature Spisula solidissima oocytes to divalent ionophore A23187. Meiosis continued to completion in the presence of the ionophore; however, parthenogenetic development of the gametes did not occur. Incubation of oocytes in calcium deficient media (Ca++ free or Ca++Mg++ free seawater) but not Mg++ free seawater inhibited ionophore induced maturation. Effects of calcium deficiency were reversible. The results suggest that nuclear disintegration and the meiotic process induced by sperm are mediated by ionic stimuli and that the establishment of meiotic arrest is linked to the availability of calcium within the oocyte.", "contents": "Induction of nuclear breakdown and meiosis in Spisula solidissima oocytes by calcium ionophore. Induction of germinal vesicle breakdown and reinitiation of the meiotic process resulted following exposure of immature Spisula solidissima oocytes to divalent ionophore A23187. Meiosis continued to completion in the presence of the ionophore; however, parthenogenetic development of the gametes did not occur. Incubation of oocytes in calcium deficient media (Ca++ free or Ca++Mg++ free seawater) but not Mg++ free seawater inhibited ionophore induced maturation. Effects of calcium deficiency were reversible. The results suggest that nuclear disintegration and the meiotic process induced by sperm are mediated by ionic stimuli and that the establishment of meiotic arrest is linked to the availability of calcium within the oocyte."}
{"id": "PMID:1092806", "title": "Some additional observations relating to the mechanism of trypan blue induced teratogenesis.", "content": "The mechanism and site of teratogenic action of trypan blue on mammalian embryos was reinvestigated. The experiments to be presented include (1) an analysis of the effect of trypan blue treatment on the morphology of the early mouse egg cylinder, (2) a demonstration of the effect of dye treatment on the enzyme acid phosphatase of yolk sac epithelium using histochemical procedures. Results obtained from these experiments indicate that trypan blue injected into mothers on day 7 of gestational age leads, within 12 to 24 hours after treatment, to dramatic abnormalities in 90-95% of egg cylinders examined. The frequency of gross malformations obtained by this treatment is considerably less when litters are examined at later stages of gestation. Acid phosphatase activity in yolk sac epithelium is depressed by the dye treatment, but there is no difference between enzymatically depressed yolk sacs of malformed embryos and yolk sacs surrounding normally appearing litter mates both obtained from trypan blue treated mothers. The hypothesis that trypan blue may exert its teratogenic effect by the direct exposure of egg cylinder stages to the dye, and that some of the egg cylinders affected may subsequently repair, is recommended for further testing.", "contents": "Some additional observations relating to the mechanism of trypan blue induced teratogenesis. The mechanism and site of teratogenic action of trypan blue on mammalian embryos was reinvestigated. The experiments to be presented include (1) an analysis of the effect of trypan blue treatment on the morphology of the early mouse egg cylinder, (2) a demonstration of the effect of dye treatment on the enzyme acid phosphatase of yolk sac epithelium using histochemical procedures. Results obtained from these experiments indicate that trypan blue injected into mothers on day 7 of gestational age leads, within 12 to 24 hours after treatment, to dramatic abnormalities in 90-95% of egg cylinders examined. The frequency of gross malformations obtained by this treatment is considerably less when litters are examined at later stages of gestation. Acid phosphatase activity in yolk sac epithelium is depressed by the dye treatment, but there is no difference between enzymatically depressed yolk sacs of malformed embryos and yolk sacs surrounding normally appearing litter mates both obtained from trypan blue treated mothers. The hypothesis that trypan blue may exert its teratogenic effect by the direct exposure of egg cylinder stages to the dye, and that some of the egg cylinders affected may subsequently repair, is recommended for further testing."}
{"id": "PMID:1092807", "title": "Development and life cycle of the parthenogenetically activated sea urchin embryo.", "content": "A method is reported for inducing parthenogenetic development in eggs of the sea urchin Lytechinus pictus, a species which previously could not be artificially activated. NH4OH or the calcium ionophore A23187 are used as activating agents followed by hypertonic treatment. The ionophore is superior in activating large numbers of unfertilized eggs, whereas NH4OH produces a larger percent of embryos able to undergo gastrulation. Both feeding larvae and urchins arising from these artificially activated eggs are diploid. All individuals in which sex has been identified have been female. The viability of these completely homozygous organisms is low compared to their fertilized counterparts.", "contents": "Development and life cycle of the parthenogenetically activated sea urchin embryo. A method is reported for inducing parthenogenetic development in eggs of the sea urchin Lytechinus pictus, a species which previously could not be artificially activated. NH4OH or the calcium ionophore A23187 are used as activating agents followed by hypertonic treatment. The ionophore is superior in activating large numbers of unfertilized eggs, whereas NH4OH produces a larger percent of embryos able to undergo gastrulation. Both feeding larvae and urchins arising from these artificially activated eggs are diploid. All individuals in which sex has been identified have been female. The viability of these completely homozygous organisms is low compared to their fertilized counterparts."}
{"id": "PMID:1092813", "title": "Visual receptive fields and their images in superior colliculus of the cat.", "content": "1. The receptive fields of collicular neurons in the cat, recorded in a single microelectrode penetration, were not centered on a point in visual space, but nested eccentrically with the smaller fields displaced toward the area centralis. The eccentric nesting was not eliminated by correcting the fields for the tangent screen distortion or by making penetrations normal to the collicular surface in coronal and parasagittal planes. These findings do not support the idea that collicular cells form topographically organized columns oriented normal to the collicular surface. 2. When the receptive fields were plotted in the visual coordinate system of the collicular map, the nesting became much more concentric, suggesting that the eccentric nesting of the receptive fields in visual space was largely a product of the retinotectal coordinate transformation. 3. The profile of a collicular receptive field, plotted in the collicular visual coordinate system is called the receptive-field image. Receptive-field images tended to have oval shapes with the long axis oriented mediolaterally. Clusters of receptive-field images, plotted for single penetrations, appeared similar wherever they occurred in the collicular map, suggesting that a common pattern of neural convergence determines the geometry of the receptive-field images in all parts of the colliculus. 4. The neural substrate of the receptive-field images was examined by tracing the theoretical patterns of neural activity which a point stimulus would produce in the retinotectal system. This analysis suggested that the shape and dimensions of the receptive-field images, and consequently the receptive fields, might be accounted for in large part by the geometry of collicular dendritic fields, the dimensions of the visual receptive fields of afferent fibers, and the retinotectal coordinate transformation. 5. Because it adjusts for the retinotectal distortion of visual space, the receptive-field image may be used to outline the distribution of collicular cells excited by a point stimulus. This makes it possible to show that a point stimulus activates large-field cells in the superficial gray layer over an area of about 2.5 by 1.5 mm in the central parts of the colliculus. It is suggested that such cells may organize the directional signals required by the oculomotor system for visual orienting behavior.", "contents": "Visual receptive fields and their images in superior colliculus of the cat. 1. The receptive fields of collicular neurons in the cat, recorded in a single microelectrode penetration, were not centered on a point in visual space, but nested eccentrically with the smaller fields displaced toward the area centralis. The eccentric nesting was not eliminated by correcting the fields for the tangent screen distortion or by making penetrations normal to the collicular surface in coronal and parasagittal planes. These findings do not support the idea that collicular cells form topographically organized columns oriented normal to the collicular surface. 2. When the receptive fields were plotted in the visual coordinate system of the collicular map, the nesting became much more concentric, suggesting that the eccentric nesting of the receptive fields in visual space was largely a product of the retinotectal coordinate transformation. 3. The profile of a collicular receptive field, plotted in the collicular visual coordinate system is called the receptive-field image. Receptive-field images tended to have oval shapes with the long axis oriented mediolaterally. Clusters of receptive-field images, plotted for single penetrations, appeared similar wherever they occurred in the collicular map, suggesting that a common pattern of neural convergence determines the geometry of the receptive-field images in all parts of the colliculus. 4. The neural substrate of the receptive-field images was examined by tracing the theoretical patterns of neural activity which a point stimulus would produce in the retinotectal system. This analysis suggested that the shape and dimensions of the receptive-field images, and consequently the receptive fields, might be accounted for in large part by the geometry of collicular dendritic fields, the dimensions of the visual receptive fields of afferent fibers, and the retinotectal coordinate transformation. 5. Because it adjusts for the retinotectal distortion of visual space, the receptive-field image may be used to outline the distribution of collicular cells excited by a point stimulus. This makes it possible to show that a point stimulus activates large-field cells in the superficial gray layer over an area of about 2.5 by 1.5 mm in the central parts of the colliculus. It is suggested that such cells may organize the directional signals required by the oculomotor system for visual orienting behavior."}
{"id": "PMID:1092814", "title": "Representation of cochlea within primary auditory cortex in the cat.", "content": "The representation of sound frequency (and of the cochlear partition) within primary auditory cortex has been investigated with use of microelectrode-mapping techniques in a series of 25 anesthetized cats. Among the results were the following: 1) Within vertical penetrations into AI, best frequency and remarkably constant for successively studied neurons across the active middle and deep cortical layers. 2) There is an orderly representation of frequency (and of represented cochlear place) within AI. Frequency is rerepresented across the mediolateral dimension of the field. On an axis perpendicular to this plane of rerepresentation, best-frequency (represented cochlear place) changes as a simple function of cortical location. 3) Any given frequency band (or sector of the cochlear partition) is represented across a belt of cortex of nearly constant width that runs on a nearly straight axis across AI. 4) There is a disproportionately large cortical surface representation of the highest-frequency octaves (basal cochlea) within AI. 5) The primary and secondary field locations were somewhat variable, when referenced to cortical surface landmarks. 6) Data from long penetrations passing down the rostral bank of the posterior ectosylvian sulcus were consistent with the existence of a vertical unit of organization in AI, akin to cortical columns described in primary visual and somatosensory cortex. 7) Responses to tonal stimuli were encountered in fields dorsocaudal, caudal, ventral, and rostral to AI. There is an orderly representation of the cochlea within the field rostal to AI, with a reversal in best frequencies across its border with AI. 8) Physiological definitions of AI boundaries are consistent with their cytoarchitectonic definition. Some of the implications of these findings are discussed.", "contents": "Representation of cochlea within primary auditory cortex in the cat. The representation of sound frequency (and of the cochlear partition) within primary auditory cortex has been investigated with use of microelectrode-mapping techniques in a series of 25 anesthetized cats. Among the results were the following: 1) Within vertical penetrations into AI, best frequency and remarkably constant for successively studied neurons across the active middle and deep cortical layers. 2) There is an orderly representation of frequency (and of represented cochlear place) within AI. Frequency is rerepresented across the mediolateral dimension of the field. On an axis perpendicular to this plane of rerepresentation, best-frequency (represented cochlear place) changes as a simple function of cortical location. 3) Any given frequency band (or sector of the cochlear partition) is represented across a belt of cortex of nearly constant width that runs on a nearly straight axis across AI. 4) There is a disproportionately large cortical surface representation of the highest-frequency octaves (basal cochlea) within AI. 5) The primary and secondary field locations were somewhat variable, when referenced to cortical surface landmarks. 6) Data from long penetrations passing down the rostral bank of the posterior ectosylvian sulcus were consistent with the existence of a vertical unit of organization in AI, akin to cortical columns described in primary visual and somatosensory cortex. 7) Responses to tonal stimuli were encountered in fields dorsocaudal, caudal, ventral, and rostral to AI. There is an orderly representation of the cochlea within the field rostal to AI, with a reversal in best frequencies across its border with AI. 8) Physiological definitions of AI boundaries are consistent with their cytoarchitectonic definition. Some of the implications of these findings are discussed."}
{"id": "PMID:1092821", "title": "Apnea of prematurity. Comparative therapeutic effects of cutaneous stimulation and nasal continuous positive airway pressure.", "content": "It has been suggested that idiopathic apnea of prematurity is related to hypoxia from pulmonary instability or an immaturity of central respiratory control mechanisms. To explore these hypotheses, 18 preterm infants were studied to examine the therapeutic effects of prophylactic cutaneous stimulation (6) and continuous positive airway pressure(12). The frequency of apnea using each procedure was reduced by 35 and 69 percent, respectively. These findings constitute the basis for new therapeutic measures for treatment of idiopathic neonatal apnea.", "contents": "Apnea of prematurity. Comparative therapeutic effects of cutaneous stimulation and nasal continuous positive airway pressure. It has been suggested that idiopathic apnea of prematurity is related to hypoxia from pulmonary instability or an immaturity of central respiratory control mechanisms. To explore these hypotheses, 18 preterm infants were studied to examine the therapeutic effects of prophylactic cutaneous stimulation (6) and continuous positive airway pressure(12). The frequency of apnea using each procedure was reduced by 35 and 69 percent, respectively. These findings constitute the basis for new therapeutic measures for treatment of idiopathic neonatal apnea."}
{"id": "PMID:1092824", "title": "Minoxidil in refractory hypertension.", "content": "A life-threatening, severe, hypertension refractory to conventional antihypertensive drugs developed in a 10-year-old girl in association with a functional renal transplant. Minoxidil, a new vasodilator, proved to be a life-saving antihypertensive drug and prevented the removal of the functional transplant. During 9 months of minoxidil therapy the blood pressure remained controlled and the renal transplant function excellent. No serious side effects except a moderate hypertrichosis have been observed. It appears that minoxidil can be used to control refractory, life-threatening hypertension in children.", "contents": "Minoxidil in refractory hypertension. A life-threatening, severe, hypertension refractory to conventional antihypertensive drugs developed in a 10-year-old girl in association with a functional renal transplant. Minoxidil, a new vasodilator, proved to be a life-saving antihypertensive drug and prevented the removal of the functional transplant. During 9 months of minoxidil therapy the blood pressure remained controlled and the renal transplant function excellent. No serious side effects except a moderate hypertrichosis have been observed. It appears that minoxidil can be used to control refractory, life-threatening hypertension in children."}
{"id": "PMID:1092826", "title": "An outbreak og gastroenteritis due to E. coli 0142 in a neonatal nursery.", "content": "A nursery outbreak of gastroenteritis casued by Escherichia coli 0142/K86/H6 is described. Over a period of nine months, 59 epidemiologically linked cases of diarrhea occurred, including 21 intractable cases with four deaths. The epidemic strain, which was not agglutinated by commerical diagnostic antisera, was isolated from the hands of personnel in five instances directly incriminated hand carriage as the mode of spread. Acquisition of illness, which was especially high among low-birth-weight infants less than 17 days old, did not correlate with any treatment modality investigated and appeared to be related to a host factor. Noninvasive small intestinal colonization, production of enterotoxin, and multiple antibiotic resistance of the epidemic strain were demonstrated and helped to explain the intractability of clinical illness in many infants, despite intensive parenteral antibiotic therapy. Surveys of fecal coliforms on the hands of nursery personnel revealed no change in prevalence after introduction of a policy of \"triple\" handwashing with 3 percent hexachlorophene soap, but a significant decrease occurred during the use of disposable gloves. The frequent occurrence of E. coli 0142 in throat swabs of affected infants suggested that pharyngeal colonization may serve as an important diagnostic clue in E. coli diarrhea.", "contents": "An outbreak og gastroenteritis due to E. coli 0142 in a neonatal nursery. A nursery outbreak of gastroenteritis casued by Escherichia coli 0142/K86/H6 is described. Over a period of nine months, 59 epidemiologically linked cases of diarrhea occurred, including 21 intractable cases with four deaths. The epidemic strain, which was not agglutinated by commerical diagnostic antisera, was isolated from the hands of personnel in five instances directly incriminated hand carriage as the mode of spread. Acquisition of illness, which was especially high among low-birth-weight infants less than 17 days old, did not correlate with any treatment modality investigated and appeared to be related to a host factor. Noninvasive small intestinal colonization, production of enterotoxin, and multiple antibiotic resistance of the epidemic strain were demonstrated and helped to explain the intractability of clinical illness in many infants, despite intensive parenteral antibiotic therapy. Surveys of fecal coliforms on the hands of nursery personnel revealed no change in prevalence after introduction of a policy of \"triple\" handwashing with 3 percent hexachlorophene soap, but a significant decrease occurred during the use of disposable gloves. The frequent occurrence of E. coli 0142 in throat swabs of affected infants suggested that pharyngeal colonization may serve as an important diagnostic clue in E. coli diarrhea."}
{"id": "PMID:1092827", "title": "Reappraisal of kanamycin usage in neonates.", "content": "This study documents an important change in the kanamycin in susceptibilites of Escherichia coli strains cultivated from neonates. Whereas some nurseries have in the past experienced resistance rates as high as 70 per cent recent survelliance of seven North American nurseries demonstrated that 90 percent or more of E. coli strains are currently susceptible to kanamycin. Pharmacokinetic studies of 65 babies treated with either 7.5 or 10 mg/kg kanamycin doses revealed that peak serum values varied with dosage, birthweight, and chronologic age. Peak serum levels were below the desired therapeutic range in many babies treated with 7.5 mg/kg doses of kanamycin every 12 hours. Concentrations in cerebrospinal fluid specimens from 21 infants were 0.5 to 12 mu/ml after 7.5 mg/kg kanamycin doses. Calculated distribution volumes, plasma clearances, and serum half-life values were used in formulating a revised kanamycin in dosage and frequency of administration schedule.", "contents": "Reappraisal of kanamycin usage in neonates. This study documents an important change in the kanamycin in susceptibilites of Escherichia coli strains cultivated from neonates. Whereas some nurseries have in the past experienced resistance rates as high as 70 per cent recent survelliance of seven North American nurseries demonstrated that 90 percent or more of E. coli strains are currently susceptible to kanamycin. Pharmacokinetic studies of 65 babies treated with either 7.5 or 10 mg/kg kanamycin doses revealed that peak serum values varied with dosage, birthweight, and chronologic age. Peak serum levels were below the desired therapeutic range in many babies treated with 7.5 mg/kg doses of kanamycin every 12 hours. Concentrations in cerebrospinal fluid specimens from 21 infants were 0.5 to 12 mu/ml after 7.5 mg/kg kanamycin doses. Calculated distribution volumes, plasma clearances, and serum half-life values were used in formulating a revised kanamycin in dosage and frequency of administration schedule."}
{"id": "PMID:1092828", "title": "Effects of red cell extract on in vitro growth and multiplication of malarial parasites.", "content": "An extract of red blood cells was prepared from monkey blood. Red cell extract (RCE) of Aotus monkeys was beneficial for the in-vitro cultivation of 2 strains of P. falciparum. The active material(s) in Aotus RCE was not sedimented by centrifugation at 34,800 g for 1 hr. Rhesus monkey RCE improved the growth of P. knowlesi for in-vitro cultivation through 4 schizogonous cycles (88 hr).", "contents": "Effects of red cell extract on in vitro growth and multiplication of malarial parasites. An extract of red blood cells was prepared from monkey blood. Red cell extract (RCE) of Aotus monkeys was beneficial for the in-vitro cultivation of 2 strains of P. falciparum. The active material(s) in Aotus RCE was not sedimented by centrifugation at 34,800 g for 1 hr. Rhesus monkey RCE improved the growth of P. knowlesi for in-vitro cultivation through 4 schizogonous cycles (88 hr)."}
{"id": "PMID:1092829", "title": "Cell volume and DNA content of Trypanosoma lewisi and T. mega in vitro.", "content": "Two species of trypanosomes were harvested at a comparable stage in vitro development, just as cell division was ceasing and cell length had become maximal and its variability minimal. At this stage, T. mega displayed both a larger cell volume than T. lewisi (27.5 vs. 14.3 mu-2) and a greater DNA content (0.23 vs. 0.048 pg/cell).", "contents": "Cell volume and DNA content of Trypanosoma lewisi and T. mega in vitro. Two species of trypanosomes were harvested at a comparable stage in vitro development, just as cell division was ceasing and cell length had become maximal and its variability minimal. At this stage, T. mega displayed both a larger cell volume than T. lewisi (27.5 vs. 14.3 mu-2) and a greater DNA content (0.23 vs. 0.048 pg/cell)."}
{"id": "PMID:1092833", "title": "The use of removable partial denture splints in the treatment of postsurgical tooth mobility.", "content": "The theory and clinical use of removable partial denture splints has been offered as a tenable solution to the problem of postsurgical tooth mobility. The close cooperation of the periodontic and prosthetic specialties is in many cases vital to the ultimate success of treatment and longevity of the dentition. Although not presented as a panacea, it is hoped that clinicians will bear this clinical entity in mind as an option in the treatment of periodontally debilitated dentitions.", "contents": "The use of removable partial denture splints in the treatment of postsurgical tooth mobility. The theory and clinical use of removable partial denture splints has been offered as a tenable solution to the problem of postsurgical tooth mobility. The close cooperation of the periodontic and prosthetic specialties is in many cases vital to the ultimate success of treatment and longevity of the dentition. Although not presented as a panacea, it is hoped that clinicians will bear this clinical entity in mind as an option in the treatment of periodontally debilitated dentitions."}
{"id": "PMID:1092835", "title": "Synthesis and antimalarial activity of heterocyclic alkyl disulfides, thiosulfates, and dithio acid derivatives.", "content": "Based on the antimalarial activity in mice of bis(4-rho-acetamidobenzenesulfonamidophenyl) disulfide, a series of N-heterocyclic alkyl disulfides and thiosulfates was synthesized and screened for antimalarial activity. Several related dithio acid dianions and S- blocked derivatives were also screened to provide an indication of the possible role that thiol anions might play in malaria chemotherapy. Activity was limited by toxicity with these compounds, and none of those tested, with the exception of bis(4-rho-acetamidobenzenesulfonamidophenyl) disulfide, showed curative activity in either a mouse or chick test.", "contents": "Synthesis and antimalarial activity of heterocyclic alkyl disulfides, thiosulfates, and dithio acid derivatives. Based on the antimalarial activity in mice of bis(4-rho-acetamidobenzenesulfonamidophenyl) disulfide, a series of N-heterocyclic alkyl disulfides and thiosulfates was synthesized and screened for antimalarial activity. Several related dithio acid dianions and S- blocked derivatives were also screened to provide an indication of the possible role that thiol anions might play in malaria chemotherapy. Activity was limited by toxicity with these compounds, and none of those tested, with the exception of bis(4-rho-acetamidobenzenesulfonamidophenyl) disulfide, showed curative activity in either a mouse or chick test."}
{"id": "PMID:1092838", "title": "The effects of variations in the opposing dentition on changes in the partially edentulous mandible. Part III. Tooth mobility and chewing efficiency with various maxillary dentitions.", "content": "Tests were conducted using a mobilometer to determine the effects of the opposing dentition on mobility of selected teeth in the partially edentulous mandible. In addition, the effect of the opposing dentition and the presence or absence of a lower removable partial denture on chewing efficiency was evaluated. Results indicated an increase in mobility of abutment teeth following placement of removable partial dentures. The mobility decreased as the teeth became stabilized by the removable partial dentures. The amount of mucosal and bone-contour change was directly related to the degree of mobility of the abutment teeth. Comparisons in chewing efficiency indicated no particular difference between subjects with unilateral or bilateral distal-extension removable partial dentures or in the type of opposing dentition. However, the use of a lower removable partial denture does increase chewing efficiency to a level of about 50 per cent of that found for subjects with all of their natural teeth.", "contents": "The effects of variations in the opposing dentition on changes in the partially edentulous mandible. Part III. Tooth mobility and chewing efficiency with various maxillary dentitions. Tests were conducted using a mobilometer to determine the effects of the opposing dentition on mobility of selected teeth in the partially edentulous mandible. In addition, the effect of the opposing dentition and the presence or absence of a lower removable partial denture on chewing efficiency was evaluated. Results indicated an increase in mobility of abutment teeth following placement of removable partial dentures. The mobility decreased as the teeth became stabilized by the removable partial dentures. The amount of mucosal and bone-contour change was directly related to the degree of mobility of the abutment teeth. Comparisons in chewing efficiency indicated no particular difference between subjects with unilateral or bilateral distal-extension removable partial dentures or in the type of opposing dentition. However, the use of a lower removable partial denture does increase chewing efficiency to a level of about 50 per cent of that found for subjects with all of their natural teeth."}
{"id": "PMID:1092839", "title": "The processed provisional splint in periodontal prostheses.", "content": "A technique has been described whereby a heat-processed provisional splint was fabricated with the factors of esthetics and long-term serviceability being of prime importance. Additional advantages of the use of a well-adapted provisional restoration in a periodontal prosthesis are as follows: (1) They facilitate periodontal treatment by allowing total visibility and access to surgical sites when the splint is removed. (2) The splinting effect may enhance healing and periodontal-ligament reattachment by stabilizing mobile abutments. (3) The patient's ability to render adequate home care for a fixed prosthetic restoration may be evaluated and reinforced before making case restorations. (4) The patient's cooperation is assured during an extended period of restorative care when an esthetic, comfortable, provisional splint is provided.", "contents": "The processed provisional splint in periodontal prostheses. A technique has been described whereby a heat-processed provisional splint was fabricated with the factors of esthetics and long-term serviceability being of prime importance. Additional advantages of the use of a well-adapted provisional restoration in a periodontal prosthesis are as follows: (1) They facilitate periodontal treatment by allowing total visibility and access to surgical sites when the splint is removed. (2) The splinting effect may enhance healing and periodontal-ligament reattachment by stabilizing mobile abutments. (3) The patient's ability to render adequate home care for a fixed prosthetic restoration may be evaluated and reinforced before making case restorations. (4) The patient's cooperation is assured during an extended period of restorative care when an esthetic, comfortable, provisional splint is provided."}
{"id": "PMID:1092840", "title": "Complete denture technique using natural anterior teeth.", "content": "A technique has been described which permits the use of desirable natural anterior teeth in complete dentures (Fig. 3). The main characteristic of this technique is the use of cast copings with dowels to which the natural teeth are attached. The cast copings securely fit the natural teeth to the base of the denture (Fig, 4). The use of NATURAL teeth in a denture may encourage discoloration of fracture. Discoloration of the natural teeth may be prevented by the immediate removal of the pulp tissue, the attentive cleaning of the pulp chamber with normal saline, and the storage of the teeth in an atmosphere of 100 per cent humidity. Adequate vertical and horizontal overlaps insure stability of the denture and suitable protection of the natural teeth from cracknig or curshing during function of the denture. If accidental fracture or discoloration of a natural tooth should occur, ti may be replaced by an acrylic resin tooth duplicated from the natural tooth or by an artifical tooth of proper size and color.", "contents": "Complete denture technique using natural anterior teeth. A technique has been described which permits the use of desirable natural anterior teeth in complete dentures (Fig. 3). The main characteristic of this technique is the use of cast copings with dowels to which the natural teeth are attached. The cast copings securely fit the natural teeth to the base of the denture (Fig, 4). The use of NATURAL teeth in a denture may encourage discoloration of fracture. Discoloration of the natural teeth may be prevented by the immediate removal of the pulp tissue, the attentive cleaning of the pulp chamber with normal saline, and the storage of the teeth in an atmosphere of 100 per cent humidity. Adequate vertical and horizontal overlaps insure stability of the denture and suitable protection of the natural teeth from cracknig or curshing during function of the denture. If accidental fracture or discoloration of a natural tooth should occur, ti may be replaced by an acrylic resin tooth duplicated from the natural tooth or by an artifical tooth of proper size and color."}
{"id": "PMID:1092841", "title": "Methods to preclude aspiration or swallowing of dental appliances.", "content": "A technique which precludes the swallowing of dental restorations during fitting and cementation has been described. An alternative technique is to spot-weld a small loop or wire to the finished crown. Dental floss may be attached to the loop. Another method is to attach a piece of unwaxed floss to the completed crown with Eastman adhesive 910. The floss is removed after the restoration is cemented.", "contents": "Methods to preclude aspiration or swallowing of dental appliances. A technique which precludes the swallowing of dental restorations during fitting and cementation has been described. An alternative technique is to spot-weld a small loop or wire to the finished crown. Dental floss may be attached to the loop. Another method is to attach a piece of unwaxed floss to the completed crown with Eastman adhesive 910. The floss is removed after the restoration is cemented."}
{"id": "PMID:1092842", "title": "Hot-pressed porcelain process for porcelain-fused-to-metal restorations.", "content": "A technique has been described that simplifies the production of full-coverage, porcelain-fused-to-metal restorations. The process utilizes a furnace-flask system in which the restoration is invested in a high-temperature refractory material and processed at an elevated temperature (1,850 degrees F.) under pressure. Shrinkage of 0.001 to 0.005 inch at the supporting cusp tips and fossae has been recorded. Tests indicate that the porcelain produced has a tensile strength and wear rate similar to those of porcelain processed by conventional methods.", "contents": "Hot-pressed porcelain process for porcelain-fused-to-metal restorations. A technique has been described that simplifies the production of full-coverage, porcelain-fused-to-metal restorations. The process utilizes a furnace-flask system in which the restoration is invested in a high-temperature refractory material and processed at an elevated temperature (1,850 degrees F.) under pressure. Shrinkage of 0.001 to 0.005 inch at the supporting cusp tips and fossae has been recorded. Tests indicate that the porcelain produced has a tensile strength and wear rate similar to those of porcelain processed by conventional methods."}
{"id": "PMID:1092845", "title": "[Pathological, clinical and radiological study of colonic lympho-reticulosarcoma. Report of 8 cases (author's transl)].", "content": "The authors report 8 cases of lympho-reticulosarcoma of the colon and emphasize the rareness of this tumour (10 percent of cases) compared with other localisations in the stomach and small intestine. Whether primary or secondary, lymphosarcoma of the colon has various radiological appearances, depending on the mode of development of the sarcoma in the wall of the colon. Mainly sub-mucosal, it may remain localised or extend to the whole of the colon, predominating in the ileo-coecal and recto-sigmoid regions. Localised tumour forms present either in the form of large polycyclic lacunae, sometimes invaginated or as vast ulcerations with irregular nodular margin, or as due to parietal infiltration and exoluminal development of the tumour mass and neighbouring adenopathy. It is sometimes confused with carcinoma of the colon, e.g. vegetating carcinoma, colloid carcinoma, or peritoneal metastases, or with a regional abscess, e.g. appendix abscess or diverticulosis. The correct diagnosis is made on operation. The extensive colonic forms rarely take on the appearance of lymphoid pseudopolyposis, more often that of a very unusual nodular form formed of hazy lenticular lacunae. It may be confused with nodular colitis, it differs from this, however, by the absence of ulceration, changes in caliber and the persistance of normal haustration, a reticulated appearance of the mucosal outline during evacuation of the barium. In all cases, the discovery of a colonic lympho-reticulosarcoma implies complete digestive radiological investigation in order to seek gastric, duodenal or intestinal localisations, together with a search for other extra-digestive localisations. In fact, the great diffusion of the lesions modifies the prognosis and the therapeutic attitude. These lymphosarcomas and reticulosarcomas of the colon have a similar pathological and radiological appearance but differ by their sensitivity to treatment with cobalt, as reticulosarcomas are more resistant.", "contents": "[Pathological, clinical and radiological study of colonic lympho-reticulosarcoma. Report of 8 cases (author's transl)]. The authors report 8 cases of lympho-reticulosarcoma of the colon and emphasize the rareness of this tumour (10 percent of cases) compared with other localisations in the stomach and small intestine. Whether primary or secondary, lymphosarcoma of the colon has various radiological appearances, depending on the mode of development of the sarcoma in the wall of the colon. Mainly sub-mucosal, it may remain localised or extend to the whole of the colon, predominating in the ileo-coecal and recto-sigmoid regions. Localised tumour forms present either in the form of large polycyclic lacunae, sometimes invaginated or as vast ulcerations with irregular nodular margin, or as due to parietal infiltration and exoluminal development of the tumour mass and neighbouring adenopathy. It is sometimes confused with carcinoma of the colon, e.g. vegetating carcinoma, colloid carcinoma, or peritoneal metastases, or with a regional abscess, e.g. appendix abscess or diverticulosis. The correct diagnosis is made on operation. The extensive colonic forms rarely take on the appearance of lymphoid pseudopolyposis, more often that of a very unusual nodular form formed of hazy lenticular lacunae. It may be confused with nodular colitis, it differs from this, however, by the absence of ulceration, changes in caliber and the persistance of normal haustration, a reticulated appearance of the mucosal outline during evacuation of the barium. In all cases, the discovery of a colonic lympho-reticulosarcoma implies complete digestive radiological investigation in order to seek gastric, duodenal or intestinal localisations, together with a search for other extra-digestive localisations. In fact, the great diffusion of the lesions modifies the prognosis and the therapeutic attitude. These lymphosarcomas and reticulosarcomas of the colon have a similar pathological and radiological appearance but differ by their sensitivity to treatment with cobalt, as reticulosarcomas are more resistant."}
{"id": "PMID:1092846", "title": "[Osseous anomalies of centrofacial lentiginosis (39 cases) (author's transl)].", "content": "The authors present the results of an investigation on 39 cases of centrofacial lentiginosis. Eleven patients urged to be examined because of their alarming health condition, and 28 cases were found out following an inquiry within some collectivities. With centrofacial lentiginosis the cutaneous hereditary polydysplasia (characterized by typical lentigines), the dysraphic state and neuro-psychic troubles are usual and awaken the interest of the radiologist due to the frequency of various dysmorphoses, growth disturbances and a trend for arthrosis. The radiologist has to pay a due attention to these aspects representing as many manifestations of a disease of a hereditary-family type since a possible centrofacial lentiginosis also means a possible disabling potential associated with education and vocational training problems.", "contents": "[Osseous anomalies of centrofacial lentiginosis (39 cases) (author's transl)]. The authors present the results of an investigation on 39 cases of centrofacial lentiginosis. Eleven patients urged to be examined because of their alarming health condition, and 28 cases were found out following an inquiry within some collectivities. With centrofacial lentiginosis the cutaneous hereditary polydysplasia (characterized by typical lentigines), the dysraphic state and neuro-psychic troubles are usual and awaken the interest of the radiologist due to the frequency of various dysmorphoses, growth disturbances and a trend for arthrosis. The radiologist has to pay a due attention to these aspects representing as many manifestations of a disease of a hereditary-family type since a possible centrofacial lentiginosis also means a possible disabling potential associated with education and vocational training problems."}
{"id": "PMID:1092851", "title": "Progesterone levels following treatment of seasonally anoestrous ewes with synthetic LH-releasing hormone.", "content": "Plasma progesterone determinations were carried out on blood samples collected daily from Clun Forest ewes during the normal oestrous cycle and also after administration of LH-releasing hormone (LH-RH) during seasonal anoestrus. Levels of plasma progesterone at oestrus ranged from 0.1 to 0.5 ng/ml and luteal phase levels from 3 to 6 ng/ml. Levels found during seasonal anoestrus were within the range of those observed as oestrus. Following treatment with LH-RH, there was in increase in the plasma LH level in a-l cases and ovulation occurred in twenty-three out of twenty-seven treated ewes. In the animals which ovulated, the plasma progesterone concentration either remained basal (eighteen animals) or rose to a lower level (2 ng/ml is greater than) than that found during the luteal phase of the cycle.", "contents": "Progesterone levels following treatment of seasonally anoestrous ewes with synthetic LH-releasing hormone. Plasma progesterone determinations were carried out on blood samples collected daily from Clun Forest ewes during the normal oestrous cycle and also after administration of LH-releasing hormone (LH-RH) during seasonal anoestrus. Levels of plasma progesterone at oestrus ranged from 0.1 to 0.5 ng/ml and luteal phase levels from 3 to 6 ng/ml. Levels found during seasonal anoestrus were within the range of those observed as oestrus. Following treatment with LH-RH, there was in increase in the plasma LH level in a-l cases and ovulation occurred in twenty-three out of twenty-seven treated ewes. In the animals which ovulated, the plasma progesterone concentration either remained basal (eighteen animals) or rose to a lower level (2 ng/ml is greater than) than that found during the luteal phase of the cycle."}
{"id": "PMID:1092859", "title": "Properties of strains of Escherichia coli isolated from a variety of sources.", "content": "Strains of Escherichia coli causing urinary-tract infections were found to be more commonly haemolytic than were faecal strains. Strains from the introitus that subsequently gave rise to urinary-tract infections resembled urinary strains and those that did not give rise to infections resembled faecal strains. alpha-Haemolysin production was closely associated, in strains from a variety of different sources, with the production of a haemolysin detectable in liquid medium and with cytotoxicity in tissue culture.", "contents": "Properties of strains of Escherichia coli isolated from a variety of sources. Strains of Escherichia coli causing urinary-tract infections were found to be more commonly haemolytic than were faecal strains. Strains from the introitus that subsequently gave rise to urinary-tract infections resembled urinary strains and those that did not give rise to infections resembled faecal strains. alpha-Haemolysin production was closely associated, in strains from a variety of different sources, with the production of a haemolysin detectable in liquid medium and with cytotoxicity in tissue culture."}
{"id": "PMID:1092860", "title": "An erythrocyte-sensitising antigen from Vibrio cholerae.", "content": "Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases were somewhat more sensitive and more specific than those obtained in classical agglutination tests. No cross reactions occurred between V. cholerae serotypes and E. coli and S. enteritidis. Much of the reactive part of the AE was not sedimentable at 100,000 g for 1 h. The eluant from the B. cholerae AE on Sephadex G-200 yielded three fractions, one of which was the most active in IHA tests. Treatment of the AE with trypsin resulted in an appreciable increase in the heterotypic serum titres in IHA tests. The spectrophotometric absorption of the AE at 260 nm showed a hump that may have been indicative of the presence of nucleic acid. Treatment of the antigen with ribonuclease reduced its nucleic acid content but did not change to any significant extent the reactivity of the preparation. The AE antigen of V. cholerae was Molisch-positive and was capable of sensitising untanned erythrocytes in IHA tests. It is suggested that the reactive part of the AE antigen is a carbohydrate complex.", "contents": "An erythrocyte-sensitising antigen from Vibrio cholerae. Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases were somewhat more sensitive and more specific than those obtained in classical agglutination tests. No cross reactions occurred between V. cholerae serotypes and E. coli and S. enteritidis. Much of the reactive part of the AE was not sedimentable at 100,000 g for 1 h. The eluant from the B. cholerae AE on Sephadex G-200 yielded three fractions, one of which was the most active in IHA tests. Treatment of the AE with trypsin resulted in an appreciable increase in the heterotypic serum titres in IHA tests. The spectrophotometric absorption of the AE at 260 nm showed a hump that may have been indicative of the presence of nucleic acid. Treatment of the antigen with ribonuclease reduced its nucleic acid content but did not change to any significant extent the reactivity of the preparation. The AE antigen of V. cholerae was Molisch-positive and was capable of sensitising untanned erythrocytes in IHA tests. It is suggested that the reactive part of the AE antigen is a carbohydrate complex."}
{"id": "PMID:1092861", "title": "Pigment production by Bacteroides species with reference to sub-classification.", "content": "All six reference strains of Bacteroides species, 36 laboratory isolates conforming to this group, and individual strains of Escherichia coli, Proteus mirabilis, Salmonella typhimurium and Clostridum welchii produced a dense black pigment, identified as ferrus sulphide, when grown in cooked-meat media containing cystine and ferrous sulphate. This was an indicator effect resulting from the production of H2S by the bacteria in the presence of ferrous ions and was unrelated to the characteristic pigment produced by strains of B. melaninogenicus when grown on blood agar. A pigment was extracted by ultrasonic disintegration of washed cells of three reference strains of B. melanino-genicus grown for 1 week in horse-blood broth and on human-blood agar. It was intracellular or cell-associated, soluble in water and had the spectrophotometric characteristics of a derivative of haemoglobin. No such pigment was extracted from strains of B. fragilis or B. necrophorus by similar procedures. Pigment production is a stable characteristic of those strains of Bacteroides called B. melaninogenicus and it is a significant property in the classification of the Bacteroides group. However, the pigment-producing strains are not a homogenous species, and there were considerable differences between the results of biochemical tests and antibograms obtained with the three strains of B. melaninogenicus.", "contents": "Pigment production by Bacteroides species with reference to sub-classification. All six reference strains of Bacteroides species, 36 laboratory isolates conforming to this group, and individual strains of Escherichia coli, Proteus mirabilis, Salmonella typhimurium and Clostridum welchii produced a dense black pigment, identified as ferrus sulphide, when grown in cooked-meat media containing cystine and ferrous sulphate. This was an indicator effect resulting from the production of H2S by the bacteria in the presence of ferrous ions and was unrelated to the characteristic pigment produced by strains of B. melaninogenicus when grown on blood agar. A pigment was extracted by ultrasonic disintegration of washed cells of three reference strains of B. melanino-genicus grown for 1 week in horse-blood broth and on human-blood agar. It was intracellular or cell-associated, soluble in water and had the spectrophotometric characteristics of a derivative of haemoglobin. No such pigment was extracted from strains of B. fragilis or B. necrophorus by similar procedures. Pigment production is a stable characteristic of those strains of Bacteroides called B. melaninogenicus and it is a significant property in the classification of the Bacteroides group. However, the pigment-producing strains are not a homogenous species, and there were considerable differences between the results of biochemical tests and antibograms obtained with the three strains of B. melaninogenicus."}
{"id": "PMID:1092862", "title": "The pathogenesis of infections of the mouse caused by virulent and avirulent variants of an influenza virus.", "content": "A virulent variant of the normally avirulent Kunz strain of influenza virus was obtained by serial passage in mice, and the pathogenesis of the infections caused by the two strains was studied. The virulence of the passaged variant did not appear to result from increased growth in lungs, from acquisition of resistance to non-specific inhibitors or from inadequate immunogenicity, but from its greater ability to replicate in alveolar cells. The apparent adequacy of defence mechanisms to protect against mucosal infection but their failure to protect against infection of alveolar cells in discussed.", "contents": "The pathogenesis of infections of the mouse caused by virulent and avirulent variants of an influenza virus. A virulent variant of the normally avirulent Kunz strain of influenza virus was obtained by serial passage in mice, and the pathogenesis of the infections caused by the two strains was studied. The virulence of the passaged variant did not appear to result from increased growth in lungs, from acquisition of resistance to non-specific inhibitors or from inadequate immunogenicity, but from its greater ability to replicate in alveolar cells. The apparent adequacy of defence mechanisms to protect against mucosal infection but their failure to protect against infection of alveolar cells in discussed."}
{"id": "PMID:1092863", "title": "A new biotyping scheme for Salmonella typhimurium and its phylogenetic significance.", "content": "A new, two-tier system for biotyping Salmonella typhimurium gives a finer and more reliable differentiation of strains than the Kristensen scheme and is capable of future extension by the addition of new types and new tests. Strains are allocated to a primary type (1-32) by their reactions in five primary tests with Bitter's xylose medium, meso-inositol, L-rhamnose, d-tartrate and m-tartrate. Subtypes are distinguished within the primary types by reactions in ten secondary tests, which include observations for flagella and type-1 (haemagglutinating) fimbriae. Full biotypes are designated by letters indicating the subtype reactions appended to the primary-type numbers. A series of 2030 strains of S. typhimurium collected from many different sources and countries during 53 years was classified into 19 of the 32 potential primary biotypes and into 144 full biotypes. Of the series, 14% (275) were non-fimbriate inositol-nonfermenting rhamnose-nonfermenting (FIRN) strain in primary biotypes 29-32. Most other strains were fimbriate and rhamnose fermenting. Observations on several series of cultures isolated from different human or animal sources in the same epidemic showed that the biotype characters of a strain were generally stable during its growth in the natural environment and in the unselective media used for isolation and storage. Most non-fermenting strains gave rise to fermenting mutants on prolonged incubation in the substrate-containing--and therefore selective--test medium, and false-positive results from this cause were avoided by making the definitive readings of tests after a short, carefully chosen period of incubation. A genealogical tree has been drawn to show how eighteen observed primary biotypes may have evolved from a presumed archetypal ancestor of biotype 1.", "contents": "A new biotyping scheme for Salmonella typhimurium and its phylogenetic significance. A new, two-tier system for biotyping Salmonella typhimurium gives a finer and more reliable differentiation of strains than the Kristensen scheme and is capable of future extension by the addition of new types and new tests. Strains are allocated to a primary type (1-32) by their reactions in five primary tests with Bitter's xylose medium, meso-inositol, L-rhamnose, d-tartrate and m-tartrate. Subtypes are distinguished within the primary types by reactions in ten secondary tests, which include observations for flagella and type-1 (haemagglutinating) fimbriae. Full biotypes are designated by letters indicating the subtype reactions appended to the primary-type numbers. A series of 2030 strains of S. typhimurium collected from many different sources and countries during 53 years was classified into 19 of the 32 potential primary biotypes and into 144 full biotypes. Of the series, 14% (275) were non-fimbriate inositol-nonfermenting rhamnose-nonfermenting (FIRN) strain in primary biotypes 29-32. Most other strains were fimbriate and rhamnose fermenting. Observations on several series of cultures isolated from different human or animal sources in the same epidemic showed that the biotype characters of a strain were generally stable during its growth in the natural environment and in the unselective media used for isolation and storage. Most non-fermenting strains gave rise to fermenting mutants on prolonged incubation in the substrate-containing--and therefore selective--test medium, and false-positive results from this cause were avoided by making the definitive readings of tests after a short, carefully chosen period of incubation. A genealogical tree has been drawn to show how eighteen observed primary biotypes may have evolved from a presumed archetypal ancestor of biotype 1."}
{"id": "PMID:1092864", "title": "A defined medium for the growth of Clostridium tetani and other anaerobes of clinical interest.", "content": "The growth of six strains of Clostridium tetani was assessed in a chemically supplemented commercially available defined medium. All strains grew reliably even after 12 serial passages, and two strains produced demonstrable toxic activity after passage. Consistent growth of the test strains could also be obtained on a solid version of this medium (\"CA109-S\" medium), and the strains could be serially passaged on this medium. Preliminary evidence is presented that the medium supports the surface growth of some other test anaerobes. Such a defined solid medium might prove of value in further studies on the surface growth of C. tetani and of other anaerobes of clinical interest.", "contents": "A defined medium for the growth of Clostridium tetani and other anaerobes of clinical interest. The growth of six strains of Clostridium tetani was assessed in a chemically supplemented commercially available defined medium. All strains grew reliably even after 12 serial passages, and two strains produced demonstrable toxic activity after passage. Consistent growth of the test strains could also be obtained on a solid version of this medium (\"CA109-S\" medium), and the strains could be serially passaged on this medium. Preliminary evidence is presented that the medium supports the surface growth of some other test anaerobes. Such a defined solid medium might prove of value in further studies on the surface growth of C. tetani and of other anaerobes of clinical interest."}
{"id": "PMID:1092865", "title": "A simple single-step immunoimmobilisation method for the detection of Salmonella in the presence of large numbers of other bacteria.", "content": "A single-step disk immunoimmobilisation method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more senstitive than a standard method for detection of Salmonella under the experimental conditons used. The time required for detection is shortened to 24-48 h.", "contents": "A simple single-step immunoimmobilisation method for the detection of Salmonella in the presence of large numbers of other bacteria. A single-step disk immunoimmobilisation method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more senstitive than a standard method for detection of Salmonella under the experimental conditons used. The time required for detection is shortened to 24-48 h."}
{"id": "PMID:1092866", "title": "Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides.", "content": "Aeromonas hydrophila was enteropathogenic in ligated ileal loops of rabbits, causing a fluid accumulation of 1-0 - 2-0 ml per cm of gut length. Gut reaction could be produced with an inoculum as low as 10-4 viable bacteria. There was no difference in the nature of the positive reactions given by strains isolated from diarrhoeal and non-diarrhoeal children and adults and from water. Plesiomonas shigelloides, on the other hand, did nt cause a significant gut reaction. A. hydrophila multiplied in the ileal loop by about 10-5 wheras P. shigelloides did so at only 10-2-3. These experiments on a animal model thus indicated the enteropathogenic nature of A. hydrophilia, but no definite conclusion could be drawn from this study on P. shigelloides.", "contents": "Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides. Aeromonas hydrophila was enteropathogenic in ligated ileal loops of rabbits, causing a fluid accumulation of 1-0 - 2-0 ml per cm of gut length. Gut reaction could be produced with an inoculum as low as 10-4 viable bacteria. There was no difference in the nature of the positive reactions given by strains isolated from diarrhoeal and non-diarrhoeal children and adults and from water. Plesiomonas shigelloides, on the other hand, did nt cause a significant gut reaction. A. hydrophila multiplied in the ileal loop by about 10-5 wheras P. shigelloides did so at only 10-2-3. These experiments on a animal model thus indicated the enteropathogenic nature of A. hydrophilia, but no definite conclusion could be drawn from this study on P. shigelloides."}
{"id": "PMID:1092867", "title": "Lysis enhancement: a novel form of interaction between beta-lactam antibiotics.", "content": "A novel form of synergy has been observed to occur between cephalexin and certain other beta-lactam antibiotics. In the presence of cephalexin a reduction was found in the concentration of other beta-lactam agents needed to induce lysis of Escherichia coli; the effect was particularly marded when cephalexin was allowed to act for a short time before the addition of the second agent. The basis of this type of synergy--which is not likely to be of therapeutic value--is discussed in terms of a theroy perviously put forward, whcih suggests that penicillins and cephalosporins have two distinct sites of action in Gram-negative bacilli.", "contents": "Lysis enhancement: a novel form of interaction between beta-lactam antibiotics. A novel form of synergy has been observed to occur between cephalexin and certain other beta-lactam antibiotics. In the presence of cephalexin a reduction was found in the concentration of other beta-lactam agents needed to induce lysis of Escherichia coli; the effect was particularly marded when cephalexin was allowed to act for a short time before the addition of the second agent. The basis of this type of synergy--which is not likely to be of therapeutic value--is discussed in terms of a theroy perviously put forward, whcih suggests that penicillins and cephalosporins have two distinct sites of action in Gram-negative bacilli."}
{"id": "PMID:1092871", "title": "Chemotherapeutic implications of early tumor cell growth in an animal brain-tumor model.", "content": "The onset of tumor cell proliferation was determined in an animal brain-tumor model in which tumor cells had been injected into the cerebrum of rats through a hollow stainless steel screw implanted stereotactically. Tritiated thymidine uptake in the DNA at various periods after injection of tumor cells indicated no significant proliferation of tumor cells until 168-192 hours post implantation, after which tritium uptake increased exponentially. Control rats with screws alone or screws through which balanced salt solution had been injected showed an increased uptake at 72 hours, a phenomenon not observed in animals given tumor cells. Our findings suggested that, in the tumor model described, experimental chemotherapy tests should be deferred until at least 7 days post implantation.", "contents": "Chemotherapeutic implications of early tumor cell growth in an animal brain-tumor model. The onset of tumor cell proliferation was determined in an animal brain-tumor model in which tumor cells had been injected into the cerebrum of rats through a hollow stainless steel screw implanted stereotactically. Tritiated thymidine uptake in the DNA at various periods after injection of tumor cells indicated no significant proliferation of tumor cells until 168-192 hours post implantation, after which tritium uptake increased exponentially. Control rats with screws alone or screws through which balanced salt solution had been injected showed an increased uptake at 72 hours, a phenomenon not observed in animals given tumor cells. Our findings suggested that, in the tumor model described, experimental chemotherapy tests should be deferred until at least 7 days post implantation."}
{"id": "PMID:1092872", "title": "Marek's disease in immunosuppressed chickens: growth of a transplantable lymphoma and development of the disease by natural exposure.", "content": "Evidence indicated that treatment of chickens with Cytoxan at hatching (bursectomy) had little effect on the development of transplantable Marek's disease (md) lymphoma. Cytoxan treatment of chickens at 2 and 3 weeks of age, however, apparently reduced the incidence of visceral tumors in chickens naturally exposed to MD. Amelioration of MD in immunosuppressed chickens was possibly mediated through suppression of MD-induced T-cell proliferation.", "contents": "Marek's disease in immunosuppressed chickens: growth of a transplantable lymphoma and development of the disease by natural exposure. Evidence indicated that treatment of chickens with Cytoxan at hatching (bursectomy) had little effect on the development of transplantable Marek's disease (md) lymphoma. Cytoxan treatment of chickens at 2 and 3 weeks of age, however, apparently reduced the incidence of visceral tumors in chickens naturally exposed to MD. Amelioration of MD in immunosuppressed chickens was possibly mediated through suppression of MD-induced T-cell proliferation."}
{"id": "PMID:1092873", "title": "Variations in carcinoembryonic antigen localization in tumors of the colon.", "content": "The localization of carcinoembryonic antigen (CEA) in 78 tumors of the human colon was studied with the use of antibody to CEA in an indirect Coons test. The neoplasms differed morphologically and could be divided into two types: a) tissues in which CEA was localized in cells, and b) tissues in which malignant cells secreted CEA in greater or lesser quantity. In the first type of tissue, five forms of cells were observed: goblet-like, small vacuolar, limbic, those with intracellular accumulation of antigen, and diffuse. Tumor tissue secreting CEA could also be divided into five forms: holocrine-like, aprocrine-like, intraglandular, apical-basal, and lacunar-infiltrative. The change in CEA localization in pathologically altered cells were designated 'antigenic translocation.\"", "contents": "Variations in carcinoembryonic antigen localization in tumors of the colon. The localization of carcinoembryonic antigen (CEA) in 78 tumors of the human colon was studied with the use of antibody to CEA in an indirect Coons test. The neoplasms differed morphologically and could be divided into two types: a) tissues in which CEA was localized in cells, and b) tissues in which malignant cells secreted CEA in greater or lesser quantity. In the first type of tissue, five forms of cells were observed: goblet-like, small vacuolar, limbic, those with intracellular accumulation of antigen, and diffuse. Tumor tissue secreting CEA could also be divided into five forms: holocrine-like, aprocrine-like, intraglandular, apical-basal, and lacunar-infiltrative. The change in CEA localization in pathologically altered cells were designated 'antigenic translocation.\""}
{"id": "PMID:1092874", "title": "Interaction of BCG-activated macrophages with neoplastic and nonneoplastic cell lines in vitro : quantitation of the cytotoxic reaction by release of tritiated thymidine from prelabeled target cells.", "content": "Peritoneal cells from mice infected ip with Mycobacterium bovis, strain BCG, were cytotoxic to syngeneic tumor cells in vitro. Cytotoxicity was estimated by measurement of release of tritiated-thymidine (3-H-TDR) from prelabeled target cells. The cell responsible for tumor cytotoxicity was the macrophage. Macrophages from uninfected mice or from oil-, starch-, or thioglycollate-induced peritoneal exudates had little effect on labeled tumor monolayers. Tumoricidal macrophages were present at 3-7 days and persisted through 6 weeks after a single BCG injection. Two neoplastic/nonneoplastic cell-line pairs, all four of the cell lines derived from a cloned syngeneic embryo cell line, were used as target cells for BCG-activated macrophages. Both tumor cell lines released significantly more 3-H-TDR than did the two nonneoplastic lines. In a mixed neoplastic/nonneoplastic target cell population, BCG-activated macrophages selectively destroyed the neoplastic cells; nonneoplastic cells were not affected as \"innocent bystanders\".", "contents": "Interaction of BCG-activated macrophages with neoplastic and nonneoplastic cell lines in vitro : quantitation of the cytotoxic reaction by release of tritiated thymidine from prelabeled target cells. Peritoneal cells from mice infected ip with Mycobacterium bovis, strain BCG, were cytotoxic to syngeneic tumor cells in vitro. Cytotoxicity was estimated by measurement of release of tritiated-thymidine (3-H-TDR) from prelabeled target cells. The cell responsible for tumor cytotoxicity was the macrophage. Macrophages from uninfected mice or from oil-, starch-, or thioglycollate-induced peritoneal exudates had little effect on labeled tumor monolayers. Tumoricidal macrophages were present at 3-7 days and persisted through 6 weeks after a single BCG injection. Two neoplastic/nonneoplastic cell-line pairs, all four of the cell lines derived from a cloned syngeneic embryo cell line, were used as target cells for BCG-activated macrophages. Both tumor cell lines released significantly more 3-H-TDR than did the two nonneoplastic lines. In a mixed neoplastic/nonneoplastic target cell population, BCG-activated macrophages selectively destroyed the neoplastic cells; nonneoplastic cells were not affected as \"innocent bystanders\"."}
{"id": "PMID:1092877", "title": "Progressive pulmonary insufficiency and other pulmonary complications of thermal injury.", "content": "Progressive pulmonary insufficiency appears to be a universal response to the lung to a variety of injuries which damage the pulmonary-capillary emdothelium. Persistent hyperventilation, unresponsive to the administration of oxygen, is the earliest clinical sign of this complication of trauma and should prompt close monitoring of pulmonary function (measurement of arterial blood gas and pH levels, Vd/Vt A-aDo2, minute ventilation, vital capacity and inspiratory force) to assess the severity of the disease, the need for mechanical ventilatory support and the effectiveness of treatment. Other pulmonary complications of burn injury range from carbon monoxide poisoning and narcotics overdosage in the immediate postburn period through marked hyperventilation directly related to burn size occurring in the absence of significant parenchymal change to later occurring hematogenous and airborne pneumonia. Inhalation injury, a chemical tracheobronchitis which significantly increases the mortality of a given-sized burn, may be present immediately postburn but clinically inapparent for 48-72 hours. 133Xenon lung scans permit early diagnosis of this pulmonary injury and the timely institution of a graduated therapeutic response keyed to the severity of pulmonary disability. Knowledge of the pathogenesis of each of these complications is requisite for the physician caring for burn patients and permits the employment of rational preventive and therapeutic measures.", "contents": "Progressive pulmonary insufficiency and other pulmonary complications of thermal injury. Progressive pulmonary insufficiency appears to be a universal response to the lung to a variety of injuries which damage the pulmonary-capillary emdothelium. Persistent hyperventilation, unresponsive to the administration of oxygen, is the earliest clinical sign of this complication of trauma and should prompt close monitoring of pulmonary function (measurement of arterial blood gas and pH levels, Vd/Vt A-aDo2, minute ventilation, vital capacity and inspiratory force) to assess the severity of the disease, the need for mechanical ventilatory support and the effectiveness of treatment. Other pulmonary complications of burn injury range from carbon monoxide poisoning and narcotics overdosage in the immediate postburn period through marked hyperventilation directly related to burn size occurring in the absence of significant parenchymal change to later occurring hematogenous and airborne pneumonia. Inhalation injury, a chemical tracheobronchitis which significantly increases the mortality of a given-sized burn, may be present immediately postburn but clinically inapparent for 48-72 hours. 133Xenon lung scans permit early diagnosis of this pulmonary injury and the timely institution of a graduated therapeutic response keyed to the severity of pulmonary disability. Knowledge of the pathogenesis of each of these complications is requisite for the physician caring for burn patients and permits the employment of rational preventive and therapeutic measures."}
{"id": "PMID:1092878", "title": "Transnavicular perilunate dislocations.", "content": "A complex injury of the wrist, the transnavicular perilunate dislocation, is analyzed along with a review of the literature. A method of management is proposed for this injury based on experience gained with nine such injuries. Our conclusion is that open reduction and internal fixation is a necessity unless an anatomic manipulative reduction can be accomplished. It is proposed that a navicular bone graft be employed at 6 weeks if avascular necrosis of the navicular and early signs of nonunion are evident. The rationale for these proposals is discussed.", "contents": "Transnavicular perilunate dislocations. A complex injury of the wrist, the transnavicular perilunate dislocation, is analyzed along with a review of the literature. A method of management is proposed for this injury based on experience gained with nine such injuries. Our conclusion is that open reduction and internal fixation is a necessity unless an anatomic manipulative reduction can be accomplished. It is proposed that a navicular bone graft be employed at 6 weeks if avascular necrosis of the navicular and early signs of nonunion are evident. The rationale for these proposals is discussed."}
{"id": "PMID:1092884", "title": "New developments in the diagnosis and treatment of urinary tract infections.", "content": "Not long ago it was customary to expect an atmosphere of confrontation and controversy when urologic surgeons and infectious disease physicians met at conferences and symposia. Even more often each group exhibited an attitude of \"benign neglect\" towards the other. Times have changed in large part because of the efforts of the leadership group in urology. The establishment of an Advisory Board for this Journal, drawn from non-urological disciplines, is one example of the effort being made to draw together all concerned with urinary tract infections and pyelonephritis. I have been asked to review some practical up-to-date matters relating to the diagnosis and treatment of urinary tract infections and pyelonephritis from the point of view of an infectious disease physician. However, it may be helpful to begin with a synthesis of current concepts of the natural history of urinary tract infection, since the need for early diagnosis and the tactics for treatment depend largely on our expectations for the patient. This synthesis requires definition of terms that are acceptable to a wide variety of disciplines. The review would then focus on new methods for screening and office diagnosis, antimicrobial sensitivity tests, localization of infection and indications for urologic investigation, particularly in relation to vesicoureteral reflux. New developments in treatment of urinary tract infections will be discussed. Special attention will be given to areas in which we are ignorant and require more information. Last, I will direct my attention to the problem of the urinary catheter, hoping to arrive at a synthesis of the situation which will tell it like it is but avoid overstating the case.", "contents": "New developments in the diagnosis and treatment of urinary tract infections. Not long ago it was customary to expect an atmosphere of confrontation and controversy when urologic surgeons and infectious disease physicians met at conferences and symposia. Even more often each group exhibited an attitude of \"benign neglect\" towards the other. Times have changed in large part because of the efforts of the leadership group in urology. The establishment of an Advisory Board for this Journal, drawn from non-urological disciplines, is one example of the effort being made to draw together all concerned with urinary tract infections and pyelonephritis. I have been asked to review some practical up-to-date matters relating to the diagnosis and treatment of urinary tract infections and pyelonephritis from the point of view of an infectious disease physician. However, it may be helpful to begin with a synthesis of current concepts of the natural history of urinary tract infection, since the need for early diagnosis and the tactics for treatment depend largely on our expectations for the patient. This synthesis requires definition of terms that are acceptable to a wide variety of disciplines. The review would then focus on new methods for screening and office diagnosis, antimicrobial sensitivity tests, localization of infection and indications for urologic investigation, particularly in relation to vesicoureteral reflux. New developments in treatment of urinary tract infections will be discussed. Special attention will be given to areas in which we are ignorant and require more information. Last, I will direct my attention to the problem of the urinary catheter, hoping to arrive at a synthesis of the situation which will tell it like it is but avoid overstating the case."}
{"id": "PMID:1092885", "title": "Psychological factors in hypospadias repair.", "content": "Children undergoing hypospadias repair and their parents have anxieties related not only to the operation but to future expectations. These anxieties are expressed by children through their drawings and by parents through interviews. They are enhanced by the fact that the hypospadias defect is only noticeable to the parents and child and is kept as secret information. Other defects, such as cleft palate, are obvious and cannot be hidden. The anxieties associated with hypospadias appear to be different whem compared to those associated with more obvious deformities.", "contents": "Psychological factors in hypospadias repair. Children undergoing hypospadias repair and their parents have anxieties related not only to the operation but to future expectations. These anxieties are expressed by children through their drawings and by parents through interviews. They are enhanced by the fact that the hypospadias defect is only noticeable to the parents and child and is kept as secret information. Other defects, such as cleft palate, are obvious and cannot be hidden. The anxieties associated with hypospadias appear to be different whem compared to those associated with more obvious deformities."}
{"id": "PMID:1092892", "title": "Effects of gentamicin on the intestinal bacterial flora of rats.", "content": "1) Normal intestinal microbial flora (NF) in feces of rats were examined before and after administration of gentamicin (GM). Although GM caused no influence on NF after 6 hours, the number of NF decreased markedly 24 hours after GM, viz., the count of coliforms, enterococci and lactobacilli was reduced to 1/10-1/100 of the control value while proteus and fusobacteria declines in number down to 1/100-1/1,000 times as low as the control value. 2) The decreased count of NF tended to recover to some degree at the end of the 1st therapeutic week and the considerably recovered count continued thereafter indicating about 1/10 times as low as the control value until a 12-week experimental period, except that the count of lactobacilli returned to almost normal. 3) Susceptibility of coliforms isolated from GM-treated rats to GM was nearly equal to that of the bacteria isolated from the untreated control rats, which revealed no development of drug resistance to GM. 4) The increasing rate of body weight was higher in GM-treated rats than in the control, and the feed intake was consequently great in the former.", "contents": "Effects of gentamicin on the intestinal bacterial flora of rats. 1) Normal intestinal microbial flora (NF) in feces of rats were examined before and after administration of gentamicin (GM). Although GM caused no influence on NF after 6 hours, the number of NF decreased markedly 24 hours after GM, viz., the count of coliforms, enterococci and lactobacilli was reduced to 1/10-1/100 of the control value while proteus and fusobacteria declines in number down to 1/100-1/1,000 times as low as the control value. 2) The decreased count of NF tended to recover to some degree at the end of the 1st therapeutic week and the considerably recovered count continued thereafter indicating about 1/10 times as low as the control value until a 12-week experimental period, except that the count of lactobacilli returned to almost normal. 3) Susceptibility of coliforms isolated from GM-treated rats to GM was nearly equal to that of the bacteria isolated from the untreated control rats, which revealed no development of drug resistance to GM. 4) The increasing rate of body weight was higher in GM-treated rats than in the control, and the feed intake was consequently great in the former."}
{"id": "PMID:1092909", "title": "Pathophysiologic responses of the subhuman primate in experimental septic shock.", "content": "The grave clinical aspects of septic shock have stimulated the search for an experimental animal model which more closely relates to human pathophysiology. This study of the cardiovascular-pulmonary-morphologic responses of the baboon to slow infusions of live Escherichia coli organisms was designed to approximate more closely the human clinical entity. Anesthetized young adult baboons received 3-hour intravenous infusions of organisms at an average dosage of 8 times 10-9 organisms per kg. body weight. Responses of animals were followed during a period of 6 hours in the anesthetized state. There was progressive systemic hypotension and steadily decreasing cardiac output. Total peripheral resistance was uniformly depressed during the infusion, but was variable during the post-infusion survival period. Increases in heart rate, alveolar-arterial oxygen tension gradient, and oxygen uptake were uniformly present. These alterations bear close resemblance to those seen in other subhuman primates administered short term doses of live organisms. There were extensive morphologic changes in pulmonary, cardiac, and renal beds. Glomeruli contained multiple fibrin thrombi and disrupted platelets, and the glomerular capillary endothelium was focally edematous and disrupted. The myocardium exhibited capillary endothelial edema and fluid accumulation in interfiber and intrafiber spaces. There were sequestration, degranulation, and fragmentation of polymorphonuclear leukocytes and platelets, and characteristic endothelial lesions within the pulmonary vascular bed. Findings demonstrate both cardiovascular-pulmonary dysfunction and renal, cardiac, and pulmonary morphologic lesions. The baboon shock model appears to be well suited for studies of experimental septic shock and bears close resemblance to the human clinical entity.", "contents": "Pathophysiologic responses of the subhuman primate in experimental septic shock. The grave clinical aspects of septic shock have stimulated the search for an experimental animal model which more closely relates to human pathophysiology. This study of the cardiovascular-pulmonary-morphologic responses of the baboon to slow infusions of live Escherichia coli organisms was designed to approximate more closely the human clinical entity. Anesthetized young adult baboons received 3-hour intravenous infusions of organisms at an average dosage of 8 times 10-9 organisms per kg. body weight. Responses of animals were followed during a period of 6 hours in the anesthetized state. There was progressive systemic hypotension and steadily decreasing cardiac output. Total peripheral resistance was uniformly depressed during the infusion, but was variable during the post-infusion survival period. Increases in heart rate, alveolar-arterial oxygen tension gradient, and oxygen uptake were uniformly present. These alterations bear close resemblance to those seen in other subhuman primates administered short term doses of live organisms. There were extensive morphologic changes in pulmonary, cardiac, and renal beds. Glomeruli contained multiple fibrin thrombi and disrupted platelets, and the glomerular capillary endothelium was focally edematous and disrupted. The myocardium exhibited capillary endothelial edema and fluid accumulation in interfiber and intrafiber spaces. There were sequestration, degranulation, and fragmentation of polymorphonuclear leukocytes and platelets, and characteristic endothelial lesions within the pulmonary vascular bed. Findings demonstrate both cardiovascular-pulmonary dysfunction and renal, cardiac, and pulmonary morphologic lesions. The baboon shock model appears to be well suited for studies of experimental septic shock and bears close resemblance to the human clinical entity."}
{"id": "PMID:1092910", "title": "Oxygen toxicity in the newborn. The effect of prolonged 100 per cent O2 exposure on the lungs of newborn mice.", "content": "A comprhensive morphologic study of pulmonary oxygen toxicity was undertaken on 78 newborn mice of a single, highly inbred strain continuously exposed to 100 per cent oxygen at normal atmospheric pressure for 7 days. The survival rate was 95 per cent through the first 4 days and 75 per cent through the 7th day. Our findings indicate that response to continuous exposure to 100 per cent oxygen can be divided arbitrarily during the first 7 days into three different phases. During the first, or acute toxic, phase (which generally extends into the 3rd day of exposure), mechanisms, probably adaptive in nature, are sufficiently developed in some of the newborn animals to permit them to survive a continuous 7-day exposure. The acute toxic phase is followed by the critical, high mortality phase, which lasts 3 to 4 days. During this phase, which can be subdivided into a continuum of several stages, the changes seen in the initial, acute toxic phase may progress to more pronouced injury. If the injury is severe, massive pulmonary edema and hemorrhage occur, accounting probably for the deaths of most of the animals in previous similar experimental studies and of 25 per cent of our animals by the 7th day. The second stage of this high mortality phase, being more clearly reactive, consists of a progression of reactive mitochondrial changes, proliferation of granular pneumocytes, and a return to normal of the features of the membranous pneumocytes. The beginning of the third phase, the chronic injury and repair phase, is characterized by the appearance of fibroblasts and a concomitant moderate increase in the amount of collagen.", "contents": "Oxygen toxicity in the newborn. The effect of prolonged 100 per cent O2 exposure on the lungs of newborn mice. A comprhensive morphologic study of pulmonary oxygen toxicity was undertaken on 78 newborn mice of a single, highly inbred strain continuously exposed to 100 per cent oxygen at normal atmospheric pressure for 7 days. The survival rate was 95 per cent through the first 4 days and 75 per cent through the 7th day. Our findings indicate that response to continuous exposure to 100 per cent oxygen can be divided arbitrarily during the first 7 days into three different phases. During the first, or acute toxic, phase (which generally extends into the 3rd day of exposure), mechanisms, probably adaptive in nature, are sufficiently developed in some of the newborn animals to permit them to survive a continuous 7-day exposure. The acute toxic phase is followed by the critical, high mortality phase, which lasts 3 to 4 days. During this phase, which can be subdivided into a continuum of several stages, the changes seen in the initial, acute toxic phase may progress to more pronouced injury. If the injury is severe, massive pulmonary edema and hemorrhage occur, accounting probably for the deaths of most of the animals in previous similar experimental studies and of 25 per cent of our animals by the 7th day. The second stage of this high mortality phase, being more clearly reactive, consists of a progression of reactive mitochondrial changes, proliferation of granular pneumocytes, and a return to normal of the features of the membranous pneumocytes. The beginning of the third phase, the chronic injury and repair phase, is characterized by the appearance of fibroblasts and a concomitant moderate increase in the amount of collagen."}
{"id": "PMID:1092911", "title": "Progression from minimal or focal to diffuse proliferative lupus nephritis.", "content": "Histologic classification of renal glomerular lesions in 46 patients with systemic lupus erythematosus revealed 17 as having either focal proliferative (10 patients) or minimal mesangial proliferative (7 patients) glomerulonephritis. Six of the 17 have progressed to a diffuse proliferative glomerular lesion on subsequent renal biopsies, 9 months to 5 years later. Five had clinical deterioration at the time of follow-up biopsies; currently one is undergoing hemodialysis and four others have decreased renal function. Although a comparison of those who progressed with those who are stable revealed greater proteinuria in some of those who progressed, no other clinical features of the initial illness were different in the two groups, nor were differences between the two groups noted on light microscopic examination of initial renal biopsies. However, ultrastructurally, electron-dense deposits, especially those subendothelial in location, were noted along glomerular capillary basement membranes more frequently and in greater number in those who progressed. These findings suggest that lupus patients with a mild proliferative glomerulonephritis may have clinical and histologic progression of the renal disease, and those who progress cannot be clearly defined by clinical or light microscopic features. Ultrastructural demonstration of subendotheial deposits suggests a greater likelihood of subsequent progressive disease.", "contents": "Progression from minimal or focal to diffuse proliferative lupus nephritis. Histologic classification of renal glomerular lesions in 46 patients with systemic lupus erythematosus revealed 17 as having either focal proliferative (10 patients) or minimal mesangial proliferative (7 patients) glomerulonephritis. Six of the 17 have progressed to a diffuse proliferative glomerular lesion on subsequent renal biopsies, 9 months to 5 years later. Five had clinical deterioration at the time of follow-up biopsies; currently one is undergoing hemodialysis and four others have decreased renal function. Although a comparison of those who progressed with those who are stable revealed greater proteinuria in some of those who progressed, no other clinical features of the initial illness were different in the two groups, nor were differences between the two groups noted on light microscopic examination of initial renal biopsies. However, ultrastructurally, electron-dense deposits, especially those subendothelial in location, were noted along glomerular capillary basement membranes more frequently and in greater number in those who progressed. These findings suggest that lupus patients with a mild proliferative glomerulonephritis may have clinical and histologic progression of the renal disease, and those who progress cannot be clearly defined by clinical or light microscopic features. Ultrastructural demonstration of subendotheial deposits suggests a greater likelihood of subsequent progressive disease."}
{"id": "PMID:1092923", "title": "Bronchographic and angiologic observations in experimental autotransplantation of a lung or lung lobe.", "content": "Roentgenologic examinations were conducted after 260 experimental autotransplantations of a lung or its lobe in the course of a long-term dynamic observation (maximum follow-up 4 1/2 years). Accentuation and indistinctness of the pulmonary pattern were observed in combination with small focal shadows in the early post-transplant period (up to 15 days) in dogs with an uneventful course. Dynamic observations (maximum follow-up 4 1/2 years) revealed no changes in the pulmonary tissue and no anatomic or functional changes in the bronchial tree and vessels. In the late period after orthotopic and heterotopic autotransplantation of the lower lobe, the changes were due to impaired topographic interrelations of the thoracic organs: Removal of the upper lobe of the intact lung into the upper part of the left pleural cavity. This phenomenon, the so-called mediastinal hernia, is facilitated by the mobility of the cranial part of the mediastinal septum in dogs. Heterotopic autotransplantation of the lower lobe in place of the removed contralateral lung resulted in no shift of the mediastinum and no changes in the autotransplanted lobe in the late follow-up period. The signs of a mediastinalhernia were less distinct than after orthotopic autotransplantation of a lung lobe.", "contents": "Bronchographic and angiologic observations in experimental autotransplantation of a lung or lung lobe. Roentgenologic examinations were conducted after 260 experimental autotransplantations of a lung or its lobe in the course of a long-term dynamic observation (maximum follow-up 4 1/2 years). Accentuation and indistinctness of the pulmonary pattern were observed in combination with small focal shadows in the early post-transplant period (up to 15 days) in dogs with an uneventful course. Dynamic observations (maximum follow-up 4 1/2 years) revealed no changes in the pulmonary tissue and no anatomic or functional changes in the bronchial tree and vessels. In the late period after orthotopic and heterotopic autotransplantation of the lower lobe, the changes were due to impaired topographic interrelations of the thoracic organs: Removal of the upper lobe of the intact lung into the upper part of the left pleural cavity. This phenomenon, the so-called mediastinal hernia, is facilitated by the mobility of the cranial part of the mediastinal septum in dogs. Heterotopic autotransplantation of the lower lobe in place of the removed contralateral lung resulted in no shift of the mediastinum and no changes in the autotransplanted lobe in the late follow-up period. The signs of a mediastinalhernia were less distinct than after orthotopic autotransplantation of a lung lobe."}
{"id": "PMID:1092924", "title": "Fatal air embolism during thoracotomy for gunshot injury to the lung. Report of a case.", "content": "Fatal coronary air embolism occurred during thoracotomy in a patient with a gunshot wound involving the hilum of the right lung. Embolism was observed during a second period of failure of heart action. Evidently, air entered the pulmonary veins from the bronchus, which was receiving positive-pressure ventilation. The literature contains reports of only 3 similar cases, but we suspect that air embolism may be responsible for death and morbidity in additional cases in which accidental or iatrogenic lung trauma has produced a pathway between the bronchial tree and the pulmonary veins.", "contents": "Fatal air embolism during thoracotomy for gunshot injury to the lung. Report of a case. Fatal coronary air embolism occurred during thoracotomy in a patient with a gunshot wound involving the hilum of the right lung. Embolism was observed during a second period of failure of heart action. Evidently, air entered the pulmonary veins from the bronchus, which was receiving positive-pressure ventilation. The literature contains reports of only 3 similar cases, but we suspect that air embolism may be responsible for death and morbidity in additional cases in which accidental or iatrogenic lung trauma has produced a pathway between the bronchial tree and the pulmonary veins."}
{"id": "PMID:1092925", "title": "An overview of research in cancer diagnosis.", "content": "The diagnostic research program of the National Cancer Institute encompasses detection or screening, methods of diagnosis, and prognosis. The present objective of this program is diagnosis of 75% of all cancers at a time when 90% of these have not metastasized. The Institute's Divison of Cancer Biology and Diagnosis has directed its attention toward general diagnostic methods (immunologic, chemical, cytologic, imaging, and endoscopic) and organ site programs (breast, lung, cervix, bowel, bladder, and pancreas). The organ site programs usually involve multiple diagnostic techniques applied to a specific organ. Screening for asymptomatic cancer seems an important immediate objective. Optimum frequency of screening should be established for individual organ sites and for combinations of sites, to determine whether multi-site screening programs can significantly reduce mortality from cancer.", "contents": "An overview of research in cancer diagnosis. The diagnostic research program of the National Cancer Institute encompasses detection or screening, methods of diagnosis, and prognosis. The present objective of this program is diagnosis of 75% of all cancers at a time when 90% of these have not metastasized. The Institute's Divison of Cancer Biology and Diagnosis has directed its attention toward general diagnostic methods (immunologic, chemical, cytologic, imaging, and endoscopic) and organ site programs (breast, lung, cervix, bowel, bladder, and pancreas). The organ site programs usually involve multiple diagnostic techniques applied to a specific organ. Screening for asymptomatic cancer seems an important immediate objective. Optimum frequency of screening should be established for individual organ sites and for combinations of sites, to determine whether multi-site screening programs can significantly reduce mortality from cancer."}
{"id": "PMID:1092926", "title": "Hemangiosarcoma of the liver. Spectrum of morphologic changes and clinical findings.", "content": "Analysis of four cases of hemangiosarcoma of the liver and review of the literature indicate that these tumors are either predominantly cystic and fairly well differentiated or are more solid and poorly differentiated. Well-differentiated hemangiosarcomas may resemble peliosis hepatis or other benign conditions. The tumor was associated disseminated intravascular coagulation and fibrinolysis syndrome in one of our cases. One of our patients had received thorium dioxide (Thorotrast) but none seemed to have been exposed to arsenicals or to vinyl chloride.", "contents": "Hemangiosarcoma of the liver. Spectrum of morphologic changes and clinical findings. Analysis of four cases of hemangiosarcoma of the liver and review of the literature indicate that these tumors are either predominantly cystic and fairly well differentiated or are more solid and poorly differentiated. Well-differentiated hemangiosarcomas may resemble peliosis hepatis or other benign conditions. The tumor was associated disseminated intravascular coagulation and fibrinolysis syndrome in one of our cases. One of our patients had received thorium dioxide (Thorotrast) but none seemed to have been exposed to arsenicals or to vinyl chloride."}
{"id": "PMID:1092930", "title": "Metabolic support for renal sodium reabsorption.", "content": "Our studies demonstrate that renal substrate metabolism may subserve several functions. 1. Substrate oxidation concerns us all, since it provides the support for the internal and external work functions of the kidney. It appears that only certain of the substrates utilized by the kidney have, as their major fate, oxidation. 2. All the substrates participate in the synthesis and turnover of intrarenal constituents. These rates remain to be quantified. In the case of free fatty acids, are their turnover rates through the intrarenal lipid pools proportional to T-Na+? If so, this phenomenon would be part of the Q-O2 minus T-Na+ correlation. Or, is lipid synthesis related to T-Na+ in a nonlinear fashion? 3. Certain substrates (lactate, glycerol, fructose, and probably free fatty acids) are readily interconverted in kidney. This phenomenon is particularly prominent when the blood concentrations of these substrates rise. Glucose or lactate are the major interconverion products, at least in vitro. Are there significant quantities of these or other products synthesized by kidney in vivo? The in vivo observations with 14-C-palmitate suggest, but do not prove, that this is the case. The prime example of a renal substrate interconversion mechanism is, of course, the one so carefully and completely elucidated by Pitts: minus NH3 production from glutamine. The further questions he has raised in this area will undoubtedly keep him and us interested and active for some time to come.", "contents": "Metabolic support for renal sodium reabsorption. Our studies demonstrate that renal substrate metabolism may subserve several functions. 1. Substrate oxidation concerns us all, since it provides the support for the internal and external work functions of the kidney. It appears that only certain of the substrates utilized by the kidney have, as their major fate, oxidation. 2. All the substrates participate in the synthesis and turnover of intrarenal constituents. These rates remain to be quantified. In the case of free fatty acids, are their turnover rates through the intrarenal lipid pools proportional to T-Na+? If so, this phenomenon would be part of the Q-O2 minus T-Na+ correlation. Or, is lipid synthesis related to T-Na+ in a nonlinear fashion? 3. Certain substrates (lactate, glycerol, fructose, and probably free fatty acids) are readily interconverted in kidney. This phenomenon is particularly prominent when the blood concentrations of these substrates rise. Glucose or lactate are the major interconverion products, at least in vitro. Are there significant quantities of these or other products synthesized by kidney in vivo? The in vivo observations with 14-C-palmitate suggest, but do not prove, that this is the case. The prime example of a renal substrate interconversion mechanism is, of course, the one so carefully and completely elucidated by Pitts: minus NH3 production from glutamine. The further questions he has raised in this area will undoubtedly keep him and us interested and active for some time to come."}
{"id": "PMID:1092931", "title": "Distribution and transport of varied substances in the dog kidney in vivo: implications with regard to metabolic activities.", "content": "Some matters bearing on renal metabolic activities have been reviewed. The distribution of solutes to various regions of the kidneys depends not only on convection by blood and tissue fluid flow but also on diffusion of the solutes in the tissues. Lipid solubility may play a significant role in tissue diffusion in that substances with significant lipid solubility may have access to more direct pathways than strictly hydrophilic substances. Glucose utilization by the kidney is significant. Glucose may contribute as much as 25 per cent and lactate, through decarboxylation, as much as 30 per cent of the renal carbon dioxide production. Together, glucose and lactate may account for about 50 per cent of the total renal carbon dioxide production. There is evidence that there is substantial lactate production by the kidney as well as gluconeogenesis. The conditions for luminal and anti-luminal cell surface interactions with D-glucose differ with regard to configuration, conformation and phlorizin affinity. Possible pathways of entry of glucose into metabolic activities are briefly considered.", "contents": "Distribution and transport of varied substances in the dog kidney in vivo: implications with regard to metabolic activities. Some matters bearing on renal metabolic activities have been reviewed. The distribution of solutes to various regions of the kidneys depends not only on convection by blood and tissue fluid flow but also on diffusion of the solutes in the tissues. Lipid solubility may play a significant role in tissue diffusion in that substances with significant lipid solubility may have access to more direct pathways than strictly hydrophilic substances. Glucose utilization by the kidney is significant. Glucose may contribute as much as 25 per cent and lactate, through decarboxylation, as much as 30 per cent of the renal carbon dioxide production. Together, glucose and lactate may account for about 50 per cent of the total renal carbon dioxide production. There is evidence that there is substantial lactate production by the kidney as well as gluconeogenesis. The conditions for luminal and anti-luminal cell surface interactions with D-glucose differ with regard to configuration, conformation and phlorizin affinity. Possible pathways of entry of glucose into metabolic activities are briefly considered."}
{"id": "PMID:1092932", "title": "Renal metabolism of citrate.", "content": "Our studies on renal handling of citrate have shown that: (1) citrate enters renal tubular cells from luminal fluid (reabsorption) and peritubular blood; (2) reabsorption becomes maximal, i.e., Tm-limited, at filtered loads 7 to 8 times the normal; (3) administration of malate stimulates net renal production of citrate, leading to release into urine (net secretion) and into peritubular blood; (4) acute metabolic alkalosis, induced while plasma citrate levels are above normal, depressess net citrate reabsorption, stimulates citrate release into peritubular blood and abolishes overall renal uptake of citrate; (5) essentially all citrate extracted by the kidney is converted to CO2 at endogenous circulating levels. This contribution is 15 per cent of the total renal CO2 production and is independent of chronic alterations in acid-base balance.", "contents": "Renal metabolism of citrate. Our studies on renal handling of citrate have shown that: (1) citrate enters renal tubular cells from luminal fluid (reabsorption) and peritubular blood; (2) reabsorption becomes maximal, i.e., Tm-limited, at filtered loads 7 to 8 times the normal; (3) administration of malate stimulates net renal production of citrate, leading to release into urine (net secretion) and into peritubular blood; (4) acute metabolic alkalosis, induced while plasma citrate levels are above normal, depressess net citrate reabsorption, stimulates citrate release into peritubular blood and abolishes overall renal uptake of citrate; (5) essentially all citrate extracted by the kidney is converted to CO2 at endogenous circulating levels. This contribution is 15 per cent of the total renal CO2 production and is independent of chronic alterations in acid-base balance."}
{"id": "PMID:1092954", "title": "Characterization of pancreatic islet monoamine oxidase.", "content": "Monoamine oxidase (MAO) is present in isolated islets of Langerhans of rabbits, golden hamsters, and rats. Tryptamine, tyramine, serotonin, and dopamine can serve as substrates for this enzyme. We compared the properties of islet and liver MAO in the rabbit. The Michaelis constant (K(m)) for tryptamine of islet MAO (6.5 times 10-5M) is greater than the K(m) of liver MAO (3 times 10-5M). The K(m) for tyramine of islet MAO (1.5 times 10-4M) is similar to the K(m) of liver MAO (1.8 times 10-4M). Islet MAO appeared to be more susceptible to heat inactivation (50 degrees C) than did liver MAO. This may be an artifact produced by the collagenase technique used in the preparation of the islets, as collagenase treatment of liver increased the thermal lability of the MAO in this tissue. Liver and islet MAO have a comparable sensitivity to MAO inhibitors such as clorgyline, deprenyl, tranylcypromine, pargyline, and harmine. The present report, along with previous reports that MAO inhibitors alter insulin secretion, suggests that islet MAO may modify insulin secretion.", "contents": "Characterization of pancreatic islet monoamine oxidase. Monoamine oxidase (MAO) is present in isolated islets of Langerhans of rabbits, golden hamsters, and rats. Tryptamine, tyramine, serotonin, and dopamine can serve as substrates for this enzyme. We compared the properties of islet and liver MAO in the rabbit. The Michaelis constant (K(m)) for tryptamine of islet MAO (6.5 times 10-5M) is greater than the K(m) of liver MAO (3 times 10-5M). The K(m) for tyramine of islet MAO (1.5 times 10-4M) is similar to the K(m) of liver MAO (1.8 times 10-4M). Islet MAO appeared to be more susceptible to heat inactivation (50 degrees C) than did liver MAO. This may be an artifact produced by the collagenase technique used in the preparation of the islets, as collagenase treatment of liver increased the thermal lability of the MAO in this tissue. Liver and islet MAO have a comparable sensitivity to MAO inhibitors such as clorgyline, deprenyl, tranylcypromine, pargyline, and harmine. The present report, along with previous reports that MAO inhibitors alter insulin secretion, suggests that islet MAO may modify insulin secretion."}
{"id": "PMID:1092955", "title": "Pituitary-gonadal function in chronic renal failure: the effect of luteinizing hormone--releasing hormone and the influence of dialysis.", "content": "Sixteen adult male patients with chronic renal failure undergoing either chronic intermittent hemodialysis (HD) or chronic intermittent peritoneal dialysis (PD) were studied both before and immediately after dialysis. The gonadotropin responses to luteinizing hormone-releasing hormone (LH-RH) was determined, revealing an excessive luteinizing hormone (LH) response with a delayed return to normal in both dialysis groups. No significant alteration in follicle-stimulating hormone (FSH) kinetics was observed. There was no significant difference in the mean gonadotropin responses to LH-RH between the HD and PD groups, and dialysis had no effect on either mean LH or FSH responses. The chronic renal failure patients with testicular atrophy had an excessive FSH response to LH-RH when compared to those patients without testosterone was significantly lower than normal. Chronic renal failure effects testicular function and testicular atrophy is associated with seminiferous tubular destruction and an excessive FSH response. Poor renal clearance may play a role in the abnormal LH response observed.", "contents": "Pituitary-gonadal function in chronic renal failure: the effect of luteinizing hormone--releasing hormone and the influence of dialysis. Sixteen adult male patients with chronic renal failure undergoing either chronic intermittent hemodialysis (HD) or chronic intermittent peritoneal dialysis (PD) were studied both before and immediately after dialysis. The gonadotropin responses to luteinizing hormone-releasing hormone (LH-RH) was determined, revealing an excessive luteinizing hormone (LH) response with a delayed return to normal in both dialysis groups. No significant alteration in follicle-stimulating hormone (FSH) kinetics was observed. There was no significant difference in the mean gonadotropin responses to LH-RH between the HD and PD groups, and dialysis had no effect on either mean LH or FSH responses. The chronic renal failure patients with testicular atrophy had an excessive FSH response to LH-RH when compared to those patients without testosterone was significantly lower than normal. Chronic renal failure effects testicular function and testicular atrophy is associated with seminiferous tubular destruction and an excessive FSH response. Poor renal clearance may play a role in the abnormal LH response observed."}
{"id": "PMID:1092969", "title": "Gamma-ray densitometry in the analysis of hemodynamic function.", "content": "An instrument has been developed to record contrast dilution curves by a noninvasive technique. Transthoracic roentgen density alterations are recorded by use of a solid state radiation detector array. A small amount of injected nonradioactive, radiopaque contrast material is distrubuted throughout the central circulation. The detector array positioned over the cardiopulmonary silhouette, records changes in density during the passage of the contrast material, and typical indicator dilution curves are transcribed. These curves reflect changes in blood flow distribution, vascular volume, cardiac function, and intrathoracic vascular shunts; and define the hemodynamic status of the circulatory system.", "contents": "Gamma-ray densitometry in the analysis of hemodynamic function. An instrument has been developed to record contrast dilution curves by a noninvasive technique. Transthoracic roentgen density alterations are recorded by use of a solid state radiation detector array. A small amount of injected nonradioactive, radiopaque contrast material is distrubuted throughout the central circulation. The detector array positioned over the cardiopulmonary silhouette, records changes in density during the passage of the contrast material, and typical indicator dilution curves are transcribed. These curves reflect changes in blood flow distribution, vascular volume, cardiac function, and intrathoracic vascular shunts; and define the hemodynamic status of the circulatory system."}
{"id": "PMID:1092973", "title": "Double blind evaluation of long acting diethylpropion hydrochloride in obese patients from a general practice.", "content": "Obese patients encountered in general practice were studied in order to determine whether a long-acting form of diethylpropion hydrochloride (Tenuate Dospan) was more effective than placebo for weight loss. One hundred and two patients completed this double blind, 16-week crossover study. During the first eight weeks, the patients treated with diethylpropion hydrochloride lost significantly (P smaller than 0.001) more weight than the patients treated with placebo (an average of 11-1 lb or 6-4% of their initial weight as compared with 6-2 lb or 3-6% of their initial weight). When the group taking diethylpropion hydrochloride crossed over to placebo for a second eight weeks, they lost an average of 1-5 lb (0-9% of their weight at the start of the second period), while the group who crossed from placebo over to diethylpropion hydrochloride lost an average of 6-7 lb (3-8%). The advantages of diethylpropion hydrochloride over placebo are statistically significant.", "contents": "Double blind evaluation of long acting diethylpropion hydrochloride in obese patients from a general practice. Obese patients encountered in general practice were studied in order to determine whether a long-acting form of diethylpropion hydrochloride (Tenuate Dospan) was more effective than placebo for weight loss. One hundred and two patients completed this double blind, 16-week crossover study. During the first eight weeks, the patients treated with diethylpropion hydrochloride lost significantly (P smaller than 0.001) more weight than the patients treated with placebo (an average of 11-1 lb or 6-4% of their initial weight as compared with 6-2 lb or 3-6% of their initial weight). When the group taking diethylpropion hydrochloride crossed over to placebo for a second eight weeks, they lost an average of 1-5 lb (0-9% of their weight at the start of the second period), while the group who crossed from placebo over to diethylpropion hydrochloride lost an average of 6-7 lb (3-8%). The advantages of diethylpropion hydrochloride over placebo are statistically significant."}
{"id": "PMID:1092970", "title": "A fully automated cardiac output analysis system.", "content": "An automatic cardiac output analysis system is described in which indicator dilution studies are performed serially without operator assistance. Indicator dye injection, blood sampling, and monitoring for air entry into the system are performed automatically through a present program sequence. Sampled blood is returned to the subject through a venous cannula resulting in an absence of blood loss. This system may be used in performing cardiac output analyses on a serial basis and in infants and small animals where blood loss is critical.", "contents": "A fully automated cardiac output analysis system. An automatic cardiac output analysis system is described in which indicator dilution studies are performed serially without operator assistance. Indicator dye injection, blood sampling, and monitoring for air entry into the system are performed automatically through a present program sequence. Sampled blood is returned to the subject through a venous cannula resulting in an absence of blood loss. This system may be used in performing cardiac output analyses on a serial basis and in infants and small animals where blood loss is critical."}
{"id": "PMID:1092975", "title": "The starch granuloma syndrome.", "content": "Starch glove powder is not entirely innocuous when introduced into the peritoneal cavity. Foreign body granulomas formed as relation to starch deposited in the peritoneal cavity may closely resemble malignant seedlings and thus mislead the surgeon in his proposed treatment of bowel cancer. In addition, the occurrence of starch granuloma peritonitis may provoke an unnecessary operation in a patient presenting with fever and peritoneal irritation a few weeks after a seemingly uncomplicated laparotomy.", "contents": "The starch granuloma syndrome. Starch glove powder is not entirely innocuous when introduced into the peritoneal cavity. Foreign body granulomas formed as relation to starch deposited in the peritoneal cavity may closely resemble malignant seedlings and thus mislead the surgeon in his proposed treatment of bowel cancer. In addition, the occurrence of starch granuloma peritonitis may provoke an unnecessary operation in a patient presenting with fever and peritoneal irritation a few weeks after a seemingly uncomplicated laparotomy."}
{"id": "PMID:1092994", "title": "[The demonstration of twitching motility in Eikenella corrodens].", "content": "In 18 out of 39 strains of E. corrodens twitching motility was observed. Spreading colonies were associated with this kind of motility.", "contents": "[The demonstration of twitching motility in Eikenella corrodens]. In 18 out of 39 strains of E. corrodens twitching motility was observed. Spreading colonies were associated with this kind of motility."}
{"id": "PMID:1092999", "title": "An x-ray diffraction study of ribosome structure.", "content": "Dense gels of E. coli 70 S ribosomes, their 50 S subunits, CM-like particles, RNP strands and their fragments, 38 S particles obtained from RNP strand folding upon addition of Mg2+ ions, and of unoriented salt-free and free rRNA sodium and magnesium salts were studied by X-ray diffraction. It was shown that under dense gel conditions RNA molecules contained in ribosomes unfolded by desalting, like all other particles considered here, have helical regions. Under these conditions free desalted RNA has no helical regions. Experimental data on X-ray scattering at medium angles were compared with the diffraction curves calculated for homogeneous prolate and oblate ellipsoids, for various ellipsoids containing a dense region or an internal cavity, and for ellipsoids containing internal periodic regions. The results indicate that the internal structure of the 50 S ribosome is periodic, i. e., its components form a periodic lattice. The lattice spacings are approximately 42 and 28 A with a 0.8g/g dry weight sample water content. When the 50 S particle water content drops below 0.2 g/g dry weight the periodic structure is disrupted. This disruption is reversible. It was shown that CM-like particles at high ionic strenght (2 M LiCl) have approximately the same internal periodicity as the 50 S particles, but in contrast they lose this periodicity at low ionic strength (10-2M tris-HCl and 5-10-3 M MgCl2).", "contents": "An x-ray diffraction study of ribosome structure. Dense gels of E. coli 70 S ribosomes, their 50 S subunits, CM-like particles, RNP strands and their fragments, 38 S particles obtained from RNP strand folding upon addition of Mg2+ ions, and of unoriented salt-free and free rRNA sodium and magnesium salts were studied by X-ray diffraction. It was shown that under dense gel conditions RNA molecules contained in ribosomes unfolded by desalting, like all other particles considered here, have helical regions. Under these conditions free desalted RNA has no helical regions. Experimental data on X-ray scattering at medium angles were compared with the diffraction curves calculated for homogeneous prolate and oblate ellipsoids, for various ellipsoids containing a dense region or an internal cavity, and for ellipsoids containing internal periodic regions. The results indicate that the internal structure of the 50 S ribosome is periodic, i. e., its components form a periodic lattice. The lattice spacings are approximately 42 and 28 A with a 0.8g/g dry weight sample water content. When the 50 S particle water content drops below 0.2 g/g dry weight the periodic structure is disrupted. This disruption is reversible. It was shown that CM-like particles at high ionic strenght (2 M LiCl) have approximately the same internal periodicity as the 50 S particles, but in contrast they lose this periodicity at low ionic strength (10-2M tris-HCl and 5-10-3 M MgCl2)."}
{"id": "PMID:1093003", "title": "The selective iodination of yeast phenylalanine transfer RNA with 125-I.", "content": "Yeast tRNA-Phe has been labelled with 125-I under conditions which conserve the tertiary structure. Significant labelling was only found to occur on specific cytidines in single stranded regions, while other cytidines in single stranded regions and all those in the double stranded region underwent iodination to a very small extent. The pattern obtained from iodine labelling satisfies the conformation of a model recently proposed for this tRNA.", "contents": "The selective iodination of yeast phenylalanine transfer RNA with 125-I. Yeast tRNA-Phe has been labelled with 125-I under conditions which conserve the tertiary structure. Significant labelling was only found to occur on specific cytidines in single stranded regions, while other cytidines in single stranded regions and all those in the double stranded region underwent iodination to a very small extent. The pattern obtained from iodine labelling satisfies the conformation of a model recently proposed for this tRNA."}
{"id": "PMID:1093004", "title": "[Reticulosarcoma-like skin lesions in phenylketonuria (author's transl)].", "content": "Reticulosarcoma-like skin lesions are described in a boy with phenylketonuria (PKU) who was observed for 11 years. Association of the lesions with PKU is indicated by their dependence on the severity of the latter and their complete healing during treatment with low phenylalanine diet.", "contents": "[Reticulosarcoma-like skin lesions in phenylketonuria (author's transl)]. Reticulosarcoma-like skin lesions are described in a boy with phenylketonuria (PKU) who was observed for 11 years. Association of the lesions with PKU is indicated by their dependence on the severity of the latter and their complete healing during treatment with low phenylalanine diet."}
{"id": "PMID:1093009", "title": "Ultraviolet reactivation and ultraviolet mutagenesis of infectious lambda DNA: strong inhibition by treatment of DNA in vitro with UV-endonuclease from Micrococcus  luteus.", "content": "UV-endonuclease from Microcossuc luteus induces single-stranded breaks in UV-irradiated DNA of phage lambda and the average length of the fragments produced (after UV-doses to DNA of 135 and 675 erg/mm2) is equal to the average spacing between pyrimidine dimers. The plaque-forming ability of UV-irradiated lambda DNA used to infect Ca++-treated uvr A6, uvrB5 or uvrC34 recipient Escherichia coli cells (but not uve+ cells) may be significantly enhanced by treatment of lambda DNA with UV-endonuclease. This enzyme strongly decreases the reactivation of UV-irradiated lambda DNA caused by UV-irradiation of uvr+ or uvrA6 Ca++-treated cells and eliminates most clear-mutations especially if mutations are analysed using Ca++-treated uvr A6 recipient cells. It is concluded that UV-endonuclease switches a significant part of potentially mutagenic pyrimidine dimers from the UV-induced \"error-prone\" repair pathway to \"error-free\" excision repair pathway.", "contents": "Ultraviolet reactivation and ultraviolet mutagenesis of infectious lambda DNA: strong inhibition by treatment of DNA in vitro with UV-endonuclease from Micrococcus  luteus. UV-endonuclease from Microcossuc luteus induces single-stranded breaks in UV-irradiated DNA of phage lambda and the average length of the fragments produced (after UV-doses to DNA of 135 and 675 erg/mm2) is equal to the average spacing between pyrimidine dimers. The plaque-forming ability of UV-irradiated lambda DNA used to infect Ca++-treated uvr A6, uvrB5 or uvrC34 recipient Escherichia coli cells (but not uve+ cells) may be significantly enhanced by treatment of lambda DNA with UV-endonuclease. This enzyme strongly decreases the reactivation of UV-irradiated lambda DNA caused by UV-irradiation of uvr+ or uvrA6 Ca++-treated cells and eliminates most clear-mutations especially if mutations are analysed using Ca++-treated uvr A6 recipient cells. It is concluded that UV-endonuclease switches a significant part of potentially mutagenic pyrimidine dimers from the UV-induced \"error-prone\" repair pathway to \"error-free\" excision repair pathway."}
{"id": "PMID:1093010", "title": "A mutant of Eshcerchia coli K-12, URT-43, with a temperature-sensitive defect at the incision step  of the excision repair mechanism.", "content": "URT-43, which has a defect in excision repair, exhibits a temperature-dependent ultraviolet survival. It was shown that URT-43 requires protein synthesis but not DNA synthesis for recovery, by examining recovery in a growth medium containing chloramphenicol or nalidixic acid. The recovery of irradiated bacteriophage lambda in URT-43 took place in a medium containing nalidixic acid at 30 degrees, but not at 41 degrees, and chloramphenicol prevented this recovery. These results seem to imply that the product of the mutated gene in URT-43 is labile. URT-43 was confirmed to have a temperature-sensitive mutation at the incision step of the excision repair mechanism by examining the nick formation of parental DNA in alkaline sucrose gradients. The release of pyrimidine dimers was reinvestigated directly by one- and two-dimensional paper-chromatography and indirectly by examining the distribution of DNA molecules synthesized after irradiation. Dimers were excised into the acid-soluble fraction when growing bacteria were incubated, but were not excised when in amino acid starved bacteria. These results suggest that URT-43 is a mutant slowly excising pyrimidine dimers because the product of a mutated gene concerned with the incision step of the excision repair mechanism is unstable.", "contents": "A mutant of Eshcerchia coli K-12, URT-43, with a temperature-sensitive defect at the incision step  of the excision repair mechanism. URT-43, which has a defect in excision repair, exhibits a temperature-dependent ultraviolet survival. It was shown that URT-43 requires protein synthesis but not DNA synthesis for recovery, by examining recovery in a growth medium containing chloramphenicol or nalidixic acid. The recovery of irradiated bacteriophage lambda in URT-43 took place in a medium containing nalidixic acid at 30 degrees, but not at 41 degrees, and chloramphenicol prevented this recovery. These results seem to imply that the product of the mutated gene in URT-43 is labile. URT-43 was confirmed to have a temperature-sensitive mutation at the incision step of the excision repair mechanism by examining the nick formation of parental DNA in alkaline sucrose gradients. The release of pyrimidine dimers was reinvestigated directly by one- and two-dimensional paper-chromatography and indirectly by examining the distribution of DNA molecules synthesized after irradiation. Dimers were excised into the acid-soluble fraction when growing bacteria were incubated, but were not excised when in amino acid starved bacteria. These results suggest that URT-43 is a mutant slowly excising pyrimidine dimers because the product of a mutated gene concerned with the incision step of the excision repair mechanism is unstable."}
{"id": "PMID:1093011", "title": "Mutagenic properties of cis-plantinum(II)diammino-dichloride in Escherichia coli.", "content": "cis-Platinum (II)diamminodichloride (PDD), an anti-tumor agent, induced auxotrophic mutations in Escherichia coli, some of which were reverted to prototrophy by exposure to PDD, 2-aminopurine (2-AP), and N-methyl-N'-nitro-N-mitrosoguanidine (NTG), but not ICR derivatives. Similarly, various 2-AT-, NTG-, and ultraviolet light-induced auxotrophs were reverted to prototrophy by PDD. Some PDD-induced auxotrophs carried nonsense mutations and others could be phenotypically suppressed growth with streptomycin. Although these findings suggest that PDD promotes base substitutions, this mutagen may also cause base subtractions because (like NTG) it induced, at reduced frequency, reversion to prototrophy of certain ICR-induced auxotrophs. Isomeric trans-platinum (II)diamminodichloride, which lacks anti-tumor activity, was an ineffective mutagen. Near-optimal conditions for PDD-induced mutagenesis entailed prolonged cultivation with low levels of mutagen where the frequency of forward mutation to auxotrophy was 10-3 and that of a selected trp isolate to prototrophy was 10-2.", "contents": "Mutagenic properties of cis-plantinum(II)diammino-dichloride in Escherichia coli. cis-Platinum (II)diamminodichloride (PDD), an anti-tumor agent, induced auxotrophic mutations in Escherichia coli, some of which were reverted to prototrophy by exposure to PDD, 2-aminopurine (2-AP), and N-methyl-N'-nitro-N-mitrosoguanidine (NTG), but not ICR derivatives. Similarly, various 2-AT-, NTG-, and ultraviolet light-induced auxotrophs were reverted to prototrophy by PDD. Some PDD-induced auxotrophs carried nonsense mutations and others could be phenotypically suppressed growth with streptomycin. Although these findings suggest that PDD promotes base substitutions, this mutagen may also cause base subtractions because (like NTG) it induced, at reduced frequency, reversion to prototrophy of certain ICR-induced auxotrophs. Isomeric trans-platinum (II)diamminodichloride, which lacks anti-tumor activity, was an ineffective mutagen. Near-optimal conditions for PDD-induced mutagenesis entailed prolonged cultivation with low levels of mutagen where the frequency of forward mutation to auxotrophy was 10-3 and that of a selected trp isolate to prototrophy was 10-2."}
{"id": "PMID:1093013", "title": "Mutagen screening with bacteria: niridazole and nitrofurans.", "content": "The mutagenic activity of nitrofuran derivatives and of niridazole is easily demonstrated by spot tests using E. coli WP2 and its uvrA derivative but not by spot tests using the S. typhimurium strains developed by Ames. Quantitative tests show that S. typhimurium TA 1538(but not TA 1535, -36 or -37) is weakly induced to revert by niridazole. However, the maximum yield of revertants is well below that obtained with E. coli WP2 uvrA. None of the S. typhimurium strains respond to the three nitrofurans tested even in quantitative tests. The S. typhimurium strains contain the reductase required for metabolic activation of the nitrofurans and treatment of a uvr+ S. typhimurium strain with niridazole or with nitrofurazone causes single-strand breaks in DNA.", "contents": "Mutagen screening with bacteria: niridazole and nitrofurans. The mutagenic activity of nitrofuran derivatives and of niridazole is easily demonstrated by spot tests using E. coli WP2 and its uvrA derivative but not by spot tests using the S. typhimurium strains developed by Ames. Quantitative tests show that S. typhimurium TA 1538(but not TA 1535, -36 or -37) is weakly induced to revert by niridazole. However, the maximum yield of revertants is well below that obtained with E. coli WP2 uvrA. None of the S. typhimurium strains respond to the three nitrofurans tested even in quantitative tests. The S. typhimurium strains contain the reductase required for metabolic activation of the nitrofurans and treatment of a uvr+ S. typhimurium strain with niridazole or with nitrofurazone causes single-strand breaks in DNA."}
{"id": "PMID:1093014", "title": "Mutagenicity of sodium hypochlorite for Salmonella typhimurium.", "content": "Sodium hypochlorite, a standard household item, induces base-substitution mutations in Salmonella typhimurium. Because of its potent bactericidal effect the mutagenicity of hypochlorite could best be demonstrated by short-term exposure to this chemical followed by the addition of ascorbic acid to decompose the hypochlorite.", "contents": "Mutagenicity of sodium hypochlorite for Salmonella typhimurium. Sodium hypochlorite, a standard household item, induces base-substitution mutations in Salmonella typhimurium. Because of its potent bactericidal effect the mutagenicity of hypochlorite could best be demonstrated by short-term exposure to this chemical followed by the addition of ascorbic acid to decompose the hypochlorite."}
{"id": "PMID:1093016", "title": "The mutagenic action of nitroimidazoles. II. Effects of 2-nitroimidazoles.", "content": "The 2-nitroimidazoles Ro-71051 (N-benzyl-2-nitro-1-imidazole-acetamide) and Ro-5-9963 (3(2-nitro-1-imidazolyl)-1,2-propanediol) increased the mutation rate of a Klebsiella pneumoniae mutant to streptomycin-resistance including streptomycin-dependence in Luria and Delbruck's fluctuation test in concentrations of 0.05-1 mM and 0.02-0.2 mM respectively. The 2-nitroimidazole, azomycin, (Ro-5-9129/001) failed to increase the mutation rate. The results are compared to those obtained with the 5-nitroimidazoles methronidazoles metronidazole, nimorazole and dimetridazole, which caused a degree of increase similar to Ro-7-1051 and Ro-59963.", "contents": "The mutagenic action of nitroimidazoles. II. Effects of 2-nitroimidazoles. The 2-nitroimidazoles Ro-71051 (N-benzyl-2-nitro-1-imidazole-acetamide) and Ro-5-9963 (3(2-nitro-1-imidazolyl)-1,2-propanediol) increased the mutation rate of a Klebsiella pneumoniae mutant to streptomycin-resistance including streptomycin-dependence in Luria and Delbruck's fluctuation test in concentrations of 0.05-1 mM and 0.02-0.2 mM respectively. The 2-nitroimidazole, azomycin, (Ro-5-9129/001) failed to increase the mutation rate. The results are compared to those obtained with the 5-nitroimidazoles methronidazoles metronidazole, nimorazole and dimetridazole, which caused a degree of increase similar to Ro-7-1051 and Ro-59963."}
{"id": "PMID:1093024", "title": "Identity and cytotoxic capacity of cells infiltrating renal allografts.", "content": "To determine the identity and cytotoxic capacity of lymphoid cells involved in allograft rejection, we studied viable, monodispersed cells recovered from 10 rejected human renal allografts. A heterogeneous population of cells including macrophages and both bone-marrow (B) and thymus-derived (T) lymphocytes accumulate in rejected grafts. Infiltrating lymphocytes exerted a specific cytolytic effect on 51Cr-labeled peripheral blood lymphocytes bearing donor antigens, ranging from 7 to 44 per cent specific lysis in nine of 10 cases. Cytolysis was closely correlated (r equal 0.91, p less than 0.05) with the histologica grade of cellular rejection but not with humoral rejection, suggesting that cytotoxic lymphocytes are an important element in cellular rejection. Limited fractionation studies showed that both T cells (in early rejection) and non-T cells (in late rejection) may produce cytotoxicity. Since as many as 50 per cent of cells recovered bore Fc receptors, the rejection process may also involve antibody-dependent target-cell lysis.", "contents": "Identity and cytotoxic capacity of cells infiltrating renal allografts. To determine the identity and cytotoxic capacity of lymphoid cells involved in allograft rejection, we studied viable, monodispersed cells recovered from 10 rejected human renal allografts. A heterogeneous population of cells including macrophages and both bone-marrow (B) and thymus-derived (T) lymphocytes accumulate in rejected grafts. Infiltrating lymphocytes exerted a specific cytolytic effect on 51Cr-labeled peripheral blood lymphocytes bearing donor antigens, ranging from 7 to 44 per cent specific lysis in nine of 10 cases. Cytolysis was closely correlated (r equal 0.91, p less than 0.05) with the histologica grade of cellular rejection but not with humoral rejection, suggesting that cytotoxic lymphocytes are an important element in cellular rejection. Limited fractionation studies showed that both T cells (in early rejection) and non-T cells (in late rejection) may produce cytotoxicity. Since as many as 50 per cent of cells recovered bore Fc receptors, the rejection process may also involve antibody-dependent target-cell lysis."}
{"id": "PMID:1093034", "title": "Effect of temperature on growth and macromolecular biosynthesis in Cryptococcus species.", "content": "Cryptococcus neoformans, a pathogenic yeast, grows at temperatures between 25 and 37 degrees C. However, the closely related non-pathogen C. albidus exhibits restricted growth at temperatures above ambient with little or no growth at 37 degrees C. The inhibition of growth of the non-pathogen, as measured by turbidity, cell number, and per cent budding, is reversible after 48 hr at the non-permissive temperature (37 degrees C). Growth cessation at 37 degrees C is accompanied by a corresponding decrease in DNA synthesis, which is not observed in C. neoformans. RNA and protein synthesis in C. albidus and C. neoformans are only slightly affected at the elevated temperature. Degradation by nucleases does not seem to account for the differences found in this cumulative DNA synthesis in C. albidus at 25 and 37 degrees C. These facts suggest that C. albidus may possess a thermo-sensitive defect in the machinery responsible for the initiation of DNA replication.", "contents": "Effect of temperature on growth and macromolecular biosynthesis in Cryptococcus species. Cryptococcus neoformans, a pathogenic yeast, grows at temperatures between 25 and 37 degrees C. However, the closely related non-pathogen C. albidus exhibits restricted growth at temperatures above ambient with little or no growth at 37 degrees C. The inhibition of growth of the non-pathogen, as measured by turbidity, cell number, and per cent budding, is reversible after 48 hr at the non-permissive temperature (37 degrees C). Growth cessation at 37 degrees C is accompanied by a corresponding decrease in DNA synthesis, which is not observed in C. neoformans. RNA and protein synthesis in C. albidus and C. neoformans are only slightly affected at the elevated temperature. Degradation by nucleases does not seem to account for the differences found in this cumulative DNA synthesis in C. albidus at 25 and 37 degrees C. These facts suggest that C. albidus may possess a thermo-sensitive defect in the machinery responsible for the initiation of DNA replication."}
{"id": "PMID:1093035", "title": "Incidence of yeasts and influence of nystatin on their control in a group of burned children.", "content": "The incidence of yeasts in the oral cavity, rectum and urine of a population of 60 children hospitalized for treatment of acute second and third degree burns was approximately the same at the time of their admission as would be expected in healthy subjects. After hospitalization, the incidence of yeasts was reduced in the intestinal tract of acute patients who received nystatin orally but increased in the oral cavity. The majority of 418 yeasts were inhibited in vitro by less than 50 units/ml nystatin and only 6 yeasts were resistant to more than 3.1 mug/ml amphotericin B. The oral cavity appeared to act as a significant reservoir from which yeasts spread to cause or contribute to the deaths of 2 of 5 patients who died during the study.", "contents": "Incidence of yeasts and influence of nystatin on their control in a group of burned children. The incidence of yeasts in the oral cavity, rectum and urine of a population of 60 children hospitalized for treatment of acute second and third degree burns was approximately the same at the time of their admission as would be expected in healthy subjects. After hospitalization, the incidence of yeasts was reduced in the intestinal tract of acute patients who received nystatin orally but increased in the oral cavity. The majority of 418 yeasts were inhibited in vitro by less than 50 units/ml nystatin and only 6 yeasts were resistant to more than 3.1 mug/ml amphotericin B. The oral cavity appeared to act as a significant reservoir from which yeasts spread to cause or contribute to the deaths of 2 of 5 patients who died during the study."}
{"id": "PMID:1093053", "title": "Uremic intoxication.", "content": "The toxic effects of metabolites that are known to accumulate in renal failure are described and the role that they may play in causing uremic symptoms is considered. The opinion of the Authors is that all they are likely to take a lesser or greater part in uremic intoxication. Methylguanidine seems to be very important in this context while for some others (like amines) nothing can be stated for studies on their chronic toxicity are lacking. The hypothesis is also considered of the accumulation of unidentified toxic metabolites with a middle molecular weight. It is stated, as to this problem, that the clinical evidence apparently supporting univocally their existence, is instead also consistent with the hypothesis of toxins (like methylguanidine) having a preferential distribution in the intracellular fluid compartment.", "contents": "Uremic intoxication. The toxic effects of metabolites that are known to accumulate in renal failure are described and the role that they may play in causing uremic symptoms is considered. The opinion of the Authors is that all they are likely to take a lesser or greater part in uremic intoxication. Methylguanidine seems to be very important in this context while for some others (like amines) nothing can be stated for studies on their chronic toxicity are lacking. The hypothesis is also considered of the accumulation of unidentified toxic metabolites with a middle molecular weight. It is stated, as to this problem, that the clinical evidence apparently supporting univocally their existence, is instead also consistent with the hypothesis of toxins (like methylguanidine) having a preferential distribution in the intracellular fluid compartment."}
{"id": "PMID:1093054", "title": "Disorders of carbohydrate and lipid metabolism in uremia.", "content": "Glucose intolerance and hypertriglyceridemia appear to be frequent metabolic concomitants of chronic uremia. Both these abnormalities are ameliorated but not eliminated by intensive hemodialysis and aggressive treatment of the uremic state. The plasma lipid alteration appears to result principally from disturbances in both the production and catabolism of the triglyceride-rich lipoproteins. The persistence of these well-recognized cardiovascular risk factors may contribute to the accelerated premature cardiovascular disease that appears to devastate chronic dialysis patients.", "contents": "Disorders of carbohydrate and lipid metabolism in uremia. Glucose intolerance and hypertriglyceridemia appear to be frequent metabolic concomitants of chronic uremia. Both these abnormalities are ameliorated but not eliminated by intensive hemodialysis and aggressive treatment of the uremic state. The plasma lipid alteration appears to result principally from disturbances in both the production and catabolism of the triglyceride-rich lipoproteins. The persistence of these well-recognized cardiovascular risk factors may contribute to the accelerated premature cardiovascular disease that appears to devastate chronic dialysis patients."}
{"id": "PMID:1093055", "title": "Calcium and phosphorus metabolism in chronic uremia.", "content": "In chronic uremia, the clinical disorders o calcium and phosphorus metabolism are influenced by the following factors: (1) intestinal absorption of calcium and phosphate, resulting in a negative calcium and phosphate balance at normal dietary intakes; (2) renal handling of calcium and phosphate: the fractional transport of calcium (the isoosmotic reabsorption taking place in the proximal tubule) is not affected by GFR modifications, whereas the Tm-limited reabsorption is severely impaired; the external phosphate balance is kept, even in the presence of a reduced nephron population, by means of a proportional reduction in TmPO4 values; (3) physiochemical state and turnover of body calcium and phosphate: in uremic patients, the distribution spaces, turnover rate of calcium, and accretion rate of bones are increased in comparison with the controls; the calcium infusion test in patients with renal osteomalacia is followed by a regular increase in plasma [PO4], whereas a significant decrease is observed in patients with renal osteitis fibrosa, due to the extreme 'avidity' of bones for calcium phosphate; the role of hyperphosphatemia is critical in keeping the plasma [Ca] lower than the expected values for a given metabolic set; moreover, an increased cell uptake of phosphate could counteract to some extent the reduced renal clearance of phosphate; (4) structural and biochemical modifications of bone tissue: uremic osteodystrophy consists mainly of two components: (a) osteomalacia, with osteoid excess, disappearance of the calcification front, and diffuse pathologic mineralization, and (b) osteitis fibrosa, with severe resorption of normally mineralized bone, slight osteoid excess, and almost normal calcification front; (5) hormonal factors: chronic stimulation of parathyroid glands may result in suppressible or even autonomous hyperparathyroidism. As to vitamin D, it has been suggested that the uremic kidney is not able to synthesize the 1,25-di-OH-cholecalciferol, the active metabolite of vitamin D: this results in an impaired intestinal absorption of calcium. On the contrary, the role of calcitonin in chronic uremia is still uncertain, since low values of plasma [Ca] are usually observed.", "contents": "Calcium and phosphorus metabolism in chronic uremia. In chronic uremia, the clinical disorders o calcium and phosphorus metabolism are influenced by the following factors: (1) intestinal absorption of calcium and phosphate, resulting in a negative calcium and phosphate balance at normal dietary intakes; (2) renal handling of calcium and phosphate: the fractional transport of calcium (the isoosmotic reabsorption taking place in the proximal tubule) is not affected by GFR modifications, whereas the Tm-limited reabsorption is severely impaired; the external phosphate balance is kept, even in the presence of a reduced nephron population, by means of a proportional reduction in TmPO4 values; (3) physiochemical state and turnover of body calcium and phosphate: in uremic patients, the distribution spaces, turnover rate of calcium, and accretion rate of bones are increased in comparison with the controls; the calcium infusion test in patients with renal osteomalacia is followed by a regular increase in plasma [PO4], whereas a significant decrease is observed in patients with renal osteitis fibrosa, due to the extreme 'avidity' of bones for calcium phosphate; the role of hyperphosphatemia is critical in keeping the plasma [Ca] lower than the expected values for a given metabolic set; moreover, an increased cell uptake of phosphate could counteract to some extent the reduced renal clearance of phosphate; (4) structural and biochemical modifications of bone tissue: uremic osteodystrophy consists mainly of two components: (a) osteomalacia, with osteoid excess, disappearance of the calcification front, and diffuse pathologic mineralization, and (b) osteitis fibrosa, with severe resorption of normally mineralized bone, slight osteoid excess, and almost normal calcification front; (5) hormonal factors: chronic stimulation of parathyroid glands may result in suppressible or even autonomous hyperparathyroidism. As to vitamin D, it has been suggested that the uremic kidney is not able to synthesize the 1,25-di-OH-cholecalciferol, the active metabolite of vitamin D: this results in an impaired intestinal absorption of calcium. On the contrary, the role of calcitonin in chronic uremia is still uncertain, since low values of plasma [Ca] are usually observed."}
{"id": "PMID:1093056", "title": "Hematologic disorders in renal failure.", "content": "Anemia is a frequent complication of renal failure. As in anemias of other origin, the resulting tissular hypoxia is partially compensated by an increased production of 2,3-diphosphoglycerate in red cells and a shift to the right of the oxygen hemoglobin dissociation curve. Two mechanisms are implicated in this anemia: increased hemolysis and depressed production of red cells. Decreased production of erythropoietin is probably the cause of reduced erythropoiesis, but the role of uremic intoxication has not been unequivocally excluded.  In the course of chronic hemodialysis, iron deficiency anemia and occasionally hypersplenism develop. It is noteworthy that blood requirements in anephric patients are two to three times greater than those of nonanephric hemodialyzed patients. Accordingly, bilateral nephrectomy should be restricted to carefully selected cases. At the present time, androgens seem to be the best treatment of renal anemia. Qualitative anomalies of platelets are the main factor responsible for uremic bleeding and are corrected by hemodialysis.", "contents": "Hematologic disorders in renal failure. Anemia is a frequent complication of renal failure. As in anemias of other origin, the resulting tissular hypoxia is partially compensated by an increased production of 2,3-diphosphoglycerate in red cells and a shift to the right of the oxygen hemoglobin dissociation curve. Two mechanisms are implicated in this anemia: increased hemolysis and depressed production of red cells. Decreased production of erythropoietin is probably the cause of reduced erythropoiesis, but the role of uremic intoxication has not been unequivocally excluded.  In the course of chronic hemodialysis, iron deficiency anemia and occasionally hypersplenism develop. It is noteworthy that blood requirements in anephric patients are two to three times greater than those of nonanephric hemodialyzed patients. Accordingly, bilateral nephrectomy should be restricted to carefully selected cases. At the present time, androgens seem to be the best treatment of renal anemia. Qualitative anomalies of platelets are the main factor responsible for uremic bleeding and are corrected by hemodialysis."}
{"id": "PMID:1093057", "title": "Treatment of stage IV carcinoma of the prostate.", "content": "Accurate diagnosis and staging of carcinoma of the prostate is essential to rational management of this disease. Once dissemination outside the periprostatic area is established, treatment is essentially limited to systemic efforts to control or suppress tumor growth and local efforts to minimize secondary effects of tumor deposits. Disseminated tumor limited to pelvic nodes constitute a possible exception to this statement since excisional and radiotherapeutic efforts to eradicate these foci may be successful. At the present time, changes in a number of objective and subjective parameters are utilized to assess the effect of therapeutic endeavors. When these are taken as a group and combined with a clinical judgement, they undoubtedly have merit. On the other hand, when utilized in a relative fashion as isolated indicators of tumor responsiveness or recurrence, their value is limited. Since most patients with disseminated carcinoma of the prostate die from their disease, critical analysis of survival data is at present likely to provide the most accurate assessment of a therapeutic  endeavor. Estrogen administration or orchiectomy seem to be the systemic measures which combine relatively limited risk of morbidity with the greatest hope of initially controlling disseminated carcinoma of the prostate. Of these, evidence suggests that low dose estrogen therapy, 1 mg stilbestrol daily, provides the best opportunity for long-term control. Although length of survival does not seem to depend on the time at which the therapy is instituted, our prejudice is usually to start treatment when dissemination is recognized. The hope of providing a longer period of a better life by this practice requires evaluation. Combining local measures such as  transurethral resection with systemic measures may add to patient comfort and longevity. Recurrent progression of tumor after initial hormonal measures in often difficult to recognize and accept. In patients with recurrent tumor activity, measures based on the concept of persistence of hormone dependence have produced disappointing results. More sophisticated selection techniques may identify a small group of patients in whom this approach is likely to produce desirable changes. For the most part chemotherapeutic agents hold greater promise of effective therapy in this group of patients.", "contents": "Treatment of stage IV carcinoma of the prostate. Accurate diagnosis and staging of carcinoma of the prostate is essential to rational management of this disease. Once dissemination outside the periprostatic area is established, treatment is essentially limited to systemic efforts to control or suppress tumor growth and local efforts to minimize secondary effects of tumor deposits. Disseminated tumor limited to pelvic nodes constitute a possible exception to this statement since excisional and radiotherapeutic efforts to eradicate these foci may be successful. At the present time, changes in a number of objective and subjective parameters are utilized to assess the effect of therapeutic endeavors. When these are taken as a group and combined with a clinical judgement, they undoubtedly have merit. On the other hand, when utilized in a relative fashion as isolated indicators of tumor responsiveness or recurrence, their value is limited. Since most patients with disseminated carcinoma of the prostate die from their disease, critical analysis of survival data is at present likely to provide the most accurate assessment of a therapeutic  endeavor. Estrogen administration or orchiectomy seem to be the systemic measures which combine relatively limited risk of morbidity with the greatest hope of initially controlling disseminated carcinoma of the prostate. Of these, evidence suggests that low dose estrogen therapy, 1 mg stilbestrol daily, provides the best opportunity for long-term control. Although length of survival does not seem to depend on the time at which the therapy is instituted, our prejudice is usually to start treatment when dissemination is recognized. The hope of providing a longer period of a better life by this practice requires evaluation. Combining local measures such as  transurethral resection with systemic measures may add to patient comfort and longevity. Recurrent progression of tumor after initial hormonal measures in often difficult to recognize and accept. In patients with recurrent tumor activity, measures based on the concept of persistence of hormone dependence have produced disappointing results. More sophisticated selection techniques may identify a small group of patients in whom this approach is likely to produce desirable changes. For the most part chemotherapeutic agents hold greater promise of effective therapy in this group of patients."}
{"id": "PMID:1093059", "title": "Chemotherapy of prostatic cancer.", "content": "Until the last few years very little information has been available regarding the potential of chemotherapy in prostatic cancer. Few drugs have been adequately tested to determine their efficacy, if any. Of the little conventional chemotherapy that has been documented, only diethylstilbestrol diphosphate (Stilphostrol, Honvan) has been safe, effective (at least in relieving bone pain) and available for repeat courses of treatment. The several well controlled clinical trials recently embarked upon and described in this article should reveal much about the effectiveness in prostatic cancer of agents already accepted in chemotherapy of other malignancies. Newer drugs will also require thorough testing. At this time no specific recommendations for chemotherpay other than the use of intravenous diethylstillbestrol diphospate can be made. Agents like estramustine phosphate (Estracyt) and flutamide (SCH-13521) with little to no bone marrow, liver, or renal toxicity are very promising. Studies of single-agent, sequential, and combined chemotherapy will necessarily lead to safe effective chemotherapy as adjuncts to surgery and/or radiotherapy for prostatic cancer in all stages.", "contents": "Chemotherapy of prostatic cancer. Until the last few years very little information has been available regarding the potential of chemotherapy in prostatic cancer. Few drugs have been adequately tested to determine their efficacy, if any. Of the little conventional chemotherapy that has been documented, only diethylstilbestrol diphosphate (Stilphostrol, Honvan) has been safe, effective (at least in relieving bone pain) and available for repeat courses of treatment. The several well controlled clinical trials recently embarked upon and described in this article should reveal much about the effectiveness in prostatic cancer of agents already accepted in chemotherapy of other malignancies. Newer drugs will also require thorough testing. At this time no specific recommendations for chemotherpay other than the use of intravenous diethylstillbestrol diphospate can be made. Agents like estramustine phosphate (Estracyt) and flutamide (SCH-13521) with little to no bone marrow, liver, or renal toxicity are very promising. Studies of single-agent, sequential, and combined chemotherapy will necessarily lead to safe effective chemotherapy as adjuncts to surgery and/or radiotherapy for prostatic cancer in all stages."}
{"id": "PMID:1093060", "title": "Hypomagnesemia following the diuresis of post-renal obstruction and renal transplant.", "content": "Three unusual cases are described which demonstrated hypomagnesemia and symptoms resembling magnesium deficiency syndrome. The hypomagnesemia was most likely secondary to urinary losses from diuresis following release of post-renal obstruction and renal homotransplantation. Heretofore, this association has not been described. Case 1 was unique because of the post-obstructive diuresis in the face of marked renal impairment. Case 2 is noteworthy because of the profound hypomagnesemia, 0.4 mEq/1, and because of the development of congestive heart failure. The rapid improvement noted in cardiac function with magnesium replacement suggests a relationship to magnesium deficiency.", "contents": "Hypomagnesemia following the diuresis of post-renal obstruction and renal transplant. Three unusual cases are described which demonstrated hypomagnesemia and symptoms resembling magnesium deficiency syndrome. The hypomagnesemia was most likely secondary to urinary losses from diuresis following release of post-renal obstruction and renal homotransplantation. Heretofore, this association has not been described. Case 1 was unique because of the post-obstructive diuresis in the face of marked renal impairment. Case 2 is noteworthy because of the profound hypomagnesemia, 0.4 mEq/1, and because of the development of congestive heart failure. The rapid improvement noted in cardiac function with magnesium replacement suggests a relationship to magnesium deficiency."}
{"id": "PMID:1093061", "title": "Uremic myocardiopathy.", "content": "The authors report on the myocardiopathy of seven patients with end-stage renal disease, characterized by fever, in four patients; tachycardia, gallop rhythm, pericardial rub, in most of patients; signs and symptoms of cardiac failure and increased cardiac area and alterations of the EKG in all patients.  Good remission of the symptoms ws achieved in two patients by hemodialysis and by successful renal transplantation in the others.  The likely etiologic factors of the syndrome are discussed.", "contents": "Uremic myocardiopathy. The authors report on the myocardiopathy of seven patients with end-stage renal disease, characterized by fever, in four patients; tachycardia, gallop rhythm, pericardial rub, in most of patients; signs and symptoms of cardiac failure and increased cardiac area and alterations of the EKG in all patients.  Good remission of the symptoms ws achieved in two patients by hemodialysis and by successful renal transplantation in the others.  The likely etiologic factors of the syndrome are discussed."}
{"id": "PMID:1093062", "title": "Number of nephrons in normal human kidneys and kidneys of patients with the congenital nephrotic syndrome.  A study using a sieving method for counting of glomeruli.", "content": "A method of graded sieving for counting of glomeruli in removed kidneys is described.  The number of nephrons (glomeruli) in six normal kidneys deriving from sutopsies of three children 22-73 months of age was 1.82 plus or minus 0.35 times 10-6.  The results give a little higher number than most previous studies with which they are compared.  In the kidneys of two 10- and 15-month-old patients with the congenital nephrotic syndrome of the Finnish type the mean number of nephrons was 3.17 plus or minus 0.52 times 10-6 which is on the average 73% higher than in the normal kidneys studied.  The number of nephrons in kidneys of five other patients showed an average of at least 3.42 plus or minus 0.30 times 10-6 the counting method being a little different.  The unusually high number of nephrons in CNF kidneys seems to be fully evident.", "contents": "Number of nephrons in normal human kidneys and kidneys of patients with the congenital nephrotic syndrome.  A study using a sieving method for counting of glomeruli. A method of graded sieving for counting of glomeruli in removed kidneys is described.  The number of nephrons (glomeruli) in six normal kidneys deriving from sutopsies of three children 22-73 months of age was 1.82 plus or minus 0.35 times 10-6.  The results give a little higher number than most previous studies with which they are compared.  In the kidneys of two 10- and 15-month-old patients with the congenital nephrotic syndrome of the Finnish type the mean number of nephrons was 3.17 plus or minus 0.52 times 10-6 which is on the average 73% higher than in the normal kidneys studied.  The number of nephrons in kidneys of five other patients showed an average of at least 3.42 plus or minus 0.30 times 10-6 the counting method being a little different.  The unusually high number of nephrons in CNF kidneys seems to be fully evident."}
{"id": "PMID:1093063", "title": "[Postoperative pulmonary complications following abdominal surgery. Study on the preventive use of a bronchosecretolytic agent associated with an antibiotic].", "content": "The factors leading to post-operative respiratory complications following abdominal surgery are examined. Data relating to a clinical study of laparotomised patients receiving a course of Bisolvomycin in the immediate postoperative period are presented. This treatment led to a marked fall in the incidence of complications, even in a group of subjects with chronic bronchopneumopathy. Its usefulness as systematic management for subjects about to undergo abdominal surgery is urged.", "contents": "[Postoperative pulmonary complications following abdominal surgery. Study on the preventive use of a bronchosecretolytic agent associated with an antibiotic]. The factors leading to post-operative respiratory complications following abdominal surgery are examined. Data relating to a clinical study of laparotomised patients receiving a course of Bisolvomycin in the immediate postoperative period are presented. This treatment led to a marked fall in the incidence of complications, even in a group of subjects with chronic bronchopneumopathy. Its usefulness as systematic management for subjects about to undergo abdominal surgery is urged."}
{"id": "PMID:1093064", "title": "[Benzbromarone in the treatment of hyperuricemias].", "content": "Benzbromarone, a synthetic benzofurane, was administered in a series of 23 patients with hyperuricaemia: 5 with gout, 8 with secondary forms, 6 with renal insufficiency, 4 undergoing diuresis with etacrinic acid. The drug was also given to 10 subjects with normal values. A surprisingly rapid and constant hypouricamising effect was noted, together with marked hyperuricuria. These changes were more prompt in the controls and relatively late and protracted in the diseased subjects, expecially those with gout. The drug was also active in renal insufficiency. Its effects ceased on suspension. It cannot be seen as a simple hyperuricuric preparation, since it may have some effect on the metabolic pathways that degrade purines to uric acid. The drug is similar to benziodarone. The replacement of iodine by two bromine atoms in its formula, however, ensures its freedom from side-effects.", "contents": "[Benzbromarone in the treatment of hyperuricemias]. Benzbromarone, a synthetic benzofurane, was administered in a series of 23 patients with hyperuricaemia: 5 with gout, 8 with secondary forms, 6 with renal insufficiency, 4 undergoing diuresis with etacrinic acid. The drug was also given to 10 subjects with normal values. A surprisingly rapid and constant hypouricamising effect was noted, together with marked hyperuricuria. These changes were more prompt in the controls and relatively late and protracted in the diseased subjects, expecially those with gout. The drug was also active in renal insufficiency. Its effects ceased on suspension. It cannot be seen as a simple hyperuricuric preparation, since it may have some effect on the metabolic pathways that degrade purines to uric acid. The drug is similar to benziodarone. The replacement of iodine by two bromine atoms in its formula, however, ensures its freedom from side-effects."}
{"id": "PMID:1093066", "title": "[Klippel-Feil syndrome and associated malformations. Comments on an unusual case].", "content": "An unusual association of Klippel-Feil syndrome and other abnormalities (Sprengel's deformity, 1st cervical and 1st dorsal spina bifida, homovertebral bone, scoliosis, plagiocephaly, basilar impression, pterygium colli, nanism, hypogenitalism, etc.) together with neurological signs of amyotrophic lateral sclerosis, in a 36-yr-old woman is reported. The clinical and radiological pictures are examined and the exterior morphology is examined. The three types of Klippel-Feil syndrome are discussed, together with the particular alterations observed in the present case: Sprengel's deformity, spina bifida, craniostenosis, basilar impression, etc.", "contents": "[Klippel-Feil syndrome and associated malformations. Comments on an unusual case]. An unusual association of Klippel-Feil syndrome and other abnormalities (Sprengel's deformity, 1st cervical and 1st dorsal spina bifida, homovertebral bone, scoliosis, plagiocephaly, basilar impression, pterygium colli, nanism, hypogenitalism, etc.) together with neurological signs of amyotrophic lateral sclerosis, in a 36-yr-old woman is reported. The clinical and radiological pictures are examined and the exterior morphology is examined. The three types of Klippel-Feil syndrome are discussed, together with the particular alterations observed in the present case: Sprengel's deformity, spina bifida, craniostenosis, basilar impression, etc."}
{"id": "PMID:1093067", "title": "[Computer study of the anatomo-clinical characteristics of breast carcinoma. II].", "content": "Data relating to the major and minor clinical features of breast cancer were examined in 200 cases and correlated to obtain an overall picture of the objective picture. The classic pattern was present in most cases. Some data, such as patient occupation and the extent of lymph node invasion expressed different meanings, while various parameters led to dubitative conclusions.", "contents": "[Computer study of the anatomo-clinical characteristics of breast carcinoma. II]. Data relating to the major and minor clinical features of breast cancer were examined in 200 cases and correlated to obtain an overall picture of the objective picture. The classic pattern was present in most cases. Some data, such as patient occupation and the extent of lymph node invasion expressed different meanings, while various parameters led to dubitative conclusions."}
{"id": "PMID:1093075", "title": "The treatment of chronic renal failure in New Zealand.", "content": "The treatment of chronic renal failure by centre haemodialysis and home haemodialysis are described. Since 1969 there has been a home dialysis programme at Christchurch. Some 63 patients were treated and 17 have died, six deaths occurred during training, four died after renal transplantation and seven died of other causes. The training time was on average 15 weeks. The implications of this form of management are discussed.", "contents": "The treatment of chronic renal failure in New Zealand. The treatment of chronic renal failure by centre haemodialysis and home haemodialysis are described. Since 1969 there has been a home dialysis programme at Christchurch. Some 63 patients were treated and 17 have died, six deaths occurred during training, four died after renal transplantation and seven died of other causes. The training time was on average 15 weeks. The implications of this form of management are discussed."}
{"id": "PMID:1093076", "title": "Pilot study on the effect of New Zealand green mussel on rheumatoid arthritis.", "content": "An extract of the New Zealand green mussel did not show any greater effect than a placebo when given to five patients with rheumatoid arthritis in a double-blind crossover trial.", "contents": "Pilot study on the effect of New Zealand green mussel on rheumatoid arthritis. An extract of the New Zealand green mussel did not show any greater effect than a placebo when given to five patients with rheumatoid arthritis in a double-blind crossover trial."}
{"id": "PMID:1093081", "title": "The early descriptions of the so-called tuning-fork tests of Weber, Rinne, Schwabach, and Bing. II. The \"Rhine Test\" and its first description by Polansky.", "content": "Concerning the first description of the so-called tuning-fork test of Rinne the literature refers to a publication by him in 1855. In this work Rinne describes, among many other observations, a method to compare air and bone conduction hearing, and adds the remark that the technique could be used for the diagnosis of deafness. It appears, however, that long before Rinne, Polansky (1842) gave a complete account of the test and its practical use. Polansky's description seems to have been forgotten. Rinne's report, too, was lost for a long period until 25 years later when Lucae (1882) and Schwabach (1885) confirmed the usefulness of the test which - in spite of its earlier discovery by Polansky - has born Rinne's name ever since.", "contents": "The early descriptions of the so-called tuning-fork tests of Weber, Rinne, Schwabach, and Bing. II. The \"Rhine Test\" and its first description by Polansky. Concerning the first description of the so-called tuning-fork test of Rinne the literature refers to a publication by him in 1855. In this work Rinne describes, among many other observations, a method to compare air and bone conduction hearing, and adds the remark that the technique could be used for the diagnosis of deafness. It appears, however, that long before Rinne, Polansky (1842) gave a complete account of the test and its practical use. Polansky's description seems to have been forgotten. Rinne's report, too, was lost for a long period until 25 years later when Lucae (1882) and Schwabach (1885) confirmed the usefulness of the test which - in spite of its earlier discovery by Polansky - has born Rinne's name ever since."}
{"id": "PMID:1093082", "title": "The early descriptions of the so-called tuning-fork tests of Weber, Rinne, Schwabach, and Bing. III. The development of the Schwabach and Bing tests.", "content": "In the third and last part of this study the development of the so-called tuning-fork tests of Schwabach and Bing is discussed. The attention is called to the work of Schmalz (1849), Lucae (1880) and Emerson (1884). These authors preceeded Schwabach (1885) in using and describing the test which became only generally accepted through Schwabach's extensive study and since then bears his name. As to the so-called Bing or occlusion test, Bing's (1891) work is reviewed and the attention is drawn to an early and apparently forgotten description of this test by Rinne (1885).", "contents": "The early descriptions of the so-called tuning-fork tests of Weber, Rinne, Schwabach, and Bing. III. The development of the Schwabach and Bing tests. In the third and last part of this study the development of the so-called tuning-fork tests of Schwabach and Bing is discussed. The attention is called to the work of Schmalz (1849), Lucae (1880) and Emerson (1884). These authors preceeded Schwabach (1885) in using and describing the test which became only generally accepted through Schwabach's extensive study and since then bears his name. As to the so-called Bing or occlusion test, Bing's (1891) work is reviewed and the attention is drawn to an early and apparently forgotten description of this test by Rinne (1885)."}
{"id": "PMID:1093083", "title": "Ernst Mach on the vestibular organ 100 years ago.", "content": "Ernst Mach (1838-1916) performed pioneering research on vestibular function 100 years ago. His experiments were mainly psychophysical and included measurements of threshold and study of the vestibular-visual interaction. Contrary to general belief, he concluded that the adequate stimulus for the semicircular canals must be pressure. He presented evidence specifically against the sustained endolymph flow theory of Breuer (1874) and Crum Brown (1874), with which he is frequently associated. Excerpts from his publications are given and their relevance to current research is discussed.", "contents": "Ernst Mach on the vestibular organ 100 years ago. Ernst Mach (1838-1916) performed pioneering research on vestibular function 100 years ago. His experiments were mainly psychophysical and included measurements of threshold and study of the vestibular-visual interaction. Contrary to general belief, he concluded that the adequate stimulus for the semicircular canals must be pressure. He presented evidence specifically against the sustained endolymph flow theory of Breuer (1874) and Crum Brown (1874), with which he is frequently associated. Excerpts from his publications are given and their relevance to current research is discussed."}
{"id": "PMID:1093087", "title": "Obstetrical paralysis.", "content": "Most patients with obstetrical paralysis have some useful functional return, and early recognition and treatment help prevent rapidly developing shoulder contractures. Initial physical therapy includes passive range of motion exercises. Fixed contractures must be released prior to reconstructive surgery designed to improve funtion. An approach to the diagnosis, evaluation, and treatment of obstetrical paralysis is given.", "contents": "Obstetrical paralysis. Most patients with obstetrical paralysis have some useful functional return, and early recognition and treatment help prevent rapidly developing shoulder contractures. Initial physical therapy includes passive range of motion exercises. Fixed contractures must be released prior to reconstructive surgery designed to improve funtion. An approach to the diagnosis, evaluation, and treatment of obstetrical paralysis is given."}
{"id": "PMID:1093088", "title": "Fractures of the proximal humeral epiphysis.", "content": "Displacements of the proximal humeral epiphysis at the epiphyseal plate result from extension injuries. The configuration of the epiphyseal plate and the thickness of the periosteum surrounding the epiphysis make slight to moderate displacements relatively stable injuries, which can be treated successfully with external support. In patients with more severe  displacement, flexion, abduction, and slight external rotation of the distal fragment bring the metaphysis into alignment with the proximal fragment, correcting the anterior angulation.  The thick periosteal sleeve attached to the physis through which the  metaphysis tears out anteriorly has great potential for remodeling the persistant bowing that remains after healing of the fracture. Although shortening and residual angulation results from closed treatment are almost always good and open surgery is rarely  indicated.", "contents": "Fractures of the proximal humeral epiphysis. Displacements of the proximal humeral epiphysis at the epiphyseal plate result from extension injuries. The configuration of the epiphyseal plate and the thickness of the periosteum surrounding the epiphysis make slight to moderate displacements relatively stable injuries, which can be treated successfully with external support. In patients with more severe  displacement, flexion, abduction, and slight external rotation of the distal fragment bring the metaphysis into alignment with the proximal fragment, correcting the anterior angulation.  The thick periosteal sleeve attached to the physis through which the  metaphysis tears out anteriorly has great potential for remodeling the persistant bowing that remains after healing of the fracture. Although shortening and residual angulation results from closed treatment are almost always good and open surgery is rarely  indicated."}
{"id": "PMID:1093095", "title": "Modern concepts of diagnosis and treatment of the thoracic outlet syndrome.", "content": "Neurovascular compression at the thoracic outlet results from one or several local anatomic abnormalities, all involving the first rib to some extent. The pathologic processes produced involve syndromes usually affecting the ulnar nerve, the subclavian artery, or the subclavian vein. The majority of these cases should be managed in a nonoperative fashion and surgical treatment reserved for patients who are unresponsive to these measures. Initial conservative nonoperative treatment with physiotherapy and patient education produces satisfactory results in 70 per cent of the patients. Operative intervention, when necessary, results in satisfactory results in 90 per cent of the cases and requires first rib resection in conjunction with repair of all other attendant mechanisms. The anterior operative approach is strongly recommended whenever vascular repair is contemplated.", "contents": "Modern concepts of diagnosis and treatment of the thoracic outlet syndrome. Neurovascular compression at the thoracic outlet results from one or several local anatomic abnormalities, all involving the first rib to some extent. The pathologic processes produced involve syndromes usually affecting the ulnar nerve, the subclavian artery, or the subclavian vein. The majority of these cases should be managed in a nonoperative fashion and surgical treatment reserved for patients who are unresponsive to these measures. Initial conservative nonoperative treatment with physiotherapy and patient education produces satisfactory results in 70 per cent of the patients. Operative intervention, when necessary, results in satisfactory results in 90 per cent of the cases and requires first rib resection in conjunction with repair of all other attendant mechanisms. The anterior operative approach is strongly recommended whenever vascular repair is contemplated."}
{"id": "PMID:1093096", "title": "Soft tissue disorders of the shoulder. Frozen shoulder, calcific tendintis, and bicipital tendinitis.", "content": "The painful periarticular conditions about the shoulder joint-calcific tendinitis, bicipital tendinitis, and frozen shoulder syndrome-are seen commonly in the general practice of medicine or in the practice of orthopedic surgery. Their etiologies are unknown. Their treatment is relatively simple and depends upon a knowledge of the anatomic structures involved and the proper use of rest and exercise. Operative intervention is rarely necessary in any of these conditions. The frozen shoulder syndrome, however, in our experience is best treated in the subacute or chronic stages by infiltration brisement under general anesthesia.", "contents": "Soft tissue disorders of the shoulder. Frozen shoulder, calcific tendintis, and bicipital tendinitis. The painful periarticular conditions about the shoulder joint-calcific tendinitis, bicipital tendinitis, and frozen shoulder syndrome-are seen commonly in the general practice of medicine or in the practice of orthopedic surgery. Their etiologies are unknown. Their treatment is relatively simple and depends upon a knowledge of the anatomic structures involved and the proper use of rest and exercise. Operative intervention is rarely necessary in any of these conditions. The frozen shoulder syndrome, however, in our experience is best treated in the subacute or chronic stages by infiltration brisement under general anesthesia."}
{"id": "PMID:1093097", "title": "Neoplasms of the shoulder girdle.", "content": "The author has briefly discussed radiation therapy, immunotherapy, and chemotherapy in the treatment of some of the more commonly encountered disease entities affecting the shoulder girdle. Specific operations and their indications are discussed. However, utilizing physical and x-ray examination, arteriography, lymphangiography, and bone scan information, the surgeon may decide that the routine operation is not indicated for a particular tumor problem. He should be able to be flexible and to modify the operation according to the specific presenting problem and not be bound by routine fixed rules. In this way the patient can be assured of the very best treatment.", "contents": "Neoplasms of the shoulder girdle. The author has briefly discussed radiation therapy, immunotherapy, and chemotherapy in the treatment of some of the more commonly encountered disease entities affecting the shoulder girdle. Specific operations and their indications are discussed. However, utilizing physical and x-ray examination, arteriography, lymphangiography, and bone scan information, the surgeon may decide that the routine operation is not indicated for a particular tumor problem. He should be able to be flexible and to modify the operation according to the specific presenting problem and not be bound by routine fixed rules. In this way the patient can be assured of the very best treatment."}
{"id": "PMID:1093103", "title": "[Study of human chromosomes using fluorescence technics].", "content": "Fluorescent techniques permitted us to draw up a system of banding of human chromosomes, using quinacrine derivatives. This procedure may be used to measure the density of the chromosomes. Recently, other techniques which use various fluorochromes after denaturation, have been developed. The latter, which use acridine orange, give remarkably reliable and reproducible results. These techniques are particularly useful for the analysis of structural changes in the chromosomes. Their use is illustrated by a certain number of observations where they permitted the authors to determine the type of structural change and identify the chromosomes involved. The method of constitution and significance of the fluorescent bands is very controversial. It is not clearly known whether the fluorochromes combine with DNA or with protein bound to the latter. In spite of this uncertainty, the existence of heterogeneity in the structure of the chromatids at the metaphase provides new information for the understanding of chromosome organisation.", "contents": "[Study of human chromosomes using fluorescence technics]. Fluorescent techniques permitted us to draw up a system of banding of human chromosomes, using quinacrine derivatives. This procedure may be used to measure the density of the chromosomes. Recently, other techniques which use various fluorochromes after denaturation, have been developed. The latter, which use acridine orange, give remarkably reliable and reproducible results. These techniques are particularly useful for the analysis of structural changes in the chromosomes. Their use is illustrated by a certain number of observations where they permitted the authors to determine the type of structural change and identify the chromosomes involved. The method of constitution and significance of the fluorescent bands is very controversial. It is not clearly known whether the fluorochromes combine with DNA or with protein bound to the latter. In spite of this uncertainty, the existence of heterogeneity in the structure of the chromatids at the metaphase provides new information for the understanding of chromosome organisation."}
{"id": "PMID:1093104", "title": "[Study of several parameters of hemostasis in Masugi's rabbit nephritis].", "content": "During experimental Masugi nephritis in the rabbit, were demonstrated various disturbances in hemostasis: a) during the initial stage: immediate, severe and transient fall in the platelet count without any change in Factor V;b)during the secondary stage, from the 7th to the 8th day onwards, increase in platelets and fibrinogen, in relation with the intensity of the nephrotic syndrome; c) in parallel, appearance of urinary fibrinogen split products in relation to the intensity of the glomerular lesions, evidence for the presence of intraglomerular fibrin. These facts confirm the role played by platelets in coagulation phenomena secondary to the immune reaction. They indicate, furthermore, the existence of hemostasis disorders during the nephrotic syndrome.", "contents": "[Study of several parameters of hemostasis in Masugi's rabbit nephritis]. During experimental Masugi nephritis in the rabbit, were demonstrated various disturbances in hemostasis: a) during the initial stage: immediate, severe and transient fall in the platelet count without any change in Factor V;b)during the secondary stage, from the 7th to the 8th day onwards, increase in platelets and fibrinogen, in relation with the intensity of the nephrotic syndrome; c) in parallel, appearance of urinary fibrinogen split products in relation to the intensity of the glomerular lesions, evidence for the presence of intraglomerular fibrin. These facts confirm the role played by platelets in coagulation phenomena secondary to the immune reaction. They indicate, furthermore, the existence of hemostasis disorders during the nephrotic syndrome."}
{"id": "PMID:1093105", "title": "[Klebsiella septicemia. Epidemiologic aspects based on antigenic and biochemical studies of strains].", "content": "50 strains of Klebsiella pneumoniae were isolated by blood cultures at Brugmann Hospital (Brussels) between 1967 and 1971. The biochemical and antigenic characteristics of the strains and their sensitivity to antibiotics were determined for epidemiological purposes. With the exception of the strains found in an hemodialysis unit, where the study showed the existence of an epidemic focus caused by serotype 28 biotype d, the strains consisted of a large number of capsular serotypes and of biotypes, excluding a single source of contamination for these septicemic infections. There is no relationship between the serotype of the strains and their sensitivity to antibiotics.", "contents": "[Klebsiella septicemia. Epidemiologic aspects based on antigenic and biochemical studies of strains]. 50 strains of Klebsiella pneumoniae were isolated by blood cultures at Brugmann Hospital (Brussels) between 1967 and 1971. The biochemical and antigenic characteristics of the strains and their sensitivity to antibiotics were determined for epidemiological purposes. With the exception of the strains found in an hemodialysis unit, where the study showed the existence of an epidemic focus caused by serotype 28 biotype d, the strains consisted of a large number of capsular serotypes and of biotypes, excluding a single source of contamination for these septicemic infections. There is no relationship between the serotype of the strains and their sensitivity to antibiotics."}
{"id": "PMID:1093106", "title": "Nocardiosis in children: report of three cases and review of the literature.", "content": "The clinical illnesses observed in three adolescents with Nocardia asteroides infection are described. The organism has been classified as a bacterium and placed in the Actinomycetaceae family. Sulfonamides remain the first choice for chemotherapy but alternative drugs are available and may be essential in successful therapy. Nocardial infections in children indicate the need for evaluation for an underlying immunological deficiency. Nocardia species should be considered as possible causative agents in immunocompromised patients with infection.", "contents": "Nocardiosis in children: report of three cases and review of the literature. The clinical illnesses observed in three adolescents with Nocardia asteroides infection are described. The organism has been classified as a bacterium and placed in the Actinomycetaceae family. Sulfonamides remain the first choice for chemotherapy but alternative drugs are available and may be essential in successful therapy. Nocardial infections in children indicate the need for evaluation for an underlying immunological deficiency. Nocardia species should be considered as possible causative agents in immunocompromised patients with infection."}
{"id": "PMID:1093107", "title": "Relationship of diet to lead poisoning in children.", "content": "Forty-six children, aged 24 to 47 months (25 controls and 21 subjects) chosen according to low and high blood lead levels respectively, were studied to ascertain the presence or absence of a relationship between dietary intake and the occurrence of plumbism (in children of low-income families).  Through single-blind interviews by a nutritionist, dietary intakes were gathered, and the average daily intake of calories, protein, and iron was calculated.  Hemoglobins, hematocrits, heights, weights, blood lead levels, and social demographic data had been gathered during the routine check for lead poisoning and at registration at the clinic.  The mean caloric and protein intake as percent of recommended dietary allowances were equal and adequate for both controls and subjects.  There was no statistically significant difference between the controls and subjects with respect to iron intake which was low in both groups.  Mean hemoglobin and hematocrit levels were in the anemic range for both groups.  The subjects were shorter and weighed less than the control group.  Pica was more prevalent among children with plumbism.  The findings of this study suggest that some factors other than dietary intake must account for the occurrence of lead poisoning in the subjects and that Blacks have a higher prevalence of plumbism in our area.", "contents": "Relationship of diet to lead poisoning in children. Forty-six children, aged 24 to 47 months (25 controls and 21 subjects) chosen according to low and high blood lead levels respectively, were studied to ascertain the presence or absence of a relationship between dietary intake and the occurrence of plumbism (in children of low-income families).  Through single-blind interviews by a nutritionist, dietary intakes were gathered, and the average daily intake of calories, protein, and iron was calculated.  Hemoglobins, hematocrits, heights, weights, blood lead levels, and social demographic data had been gathered during the routine check for lead poisoning and at registration at the clinic.  The mean caloric and protein intake as percent of recommended dietary allowances were equal and adequate for both controls and subjects.  There was no statistically significant difference between the controls and subjects with respect to iron intake which was low in both groups.  Mean hemoglobin and hematocrit levels were in the anemic range for both groups.  The subjects were shorter and weighed less than the control group.  Pica was more prevalent among children with plumbism.  The findings of this study suggest that some factors other than dietary intake must account for the occurrence of lead poisoning in the subjects and that Blacks have a higher prevalence of plumbism in our area."}
{"id": "PMID:1093108", "title": "Observations on effects of a central stimulant drug (methylphenidate) in children with hyperactive behavior.", "content": "Ninety-eight hyperactive children, ages 6 to 12 years, have been treated with methylphenidate or placebo under double-blind conditions.  Only one of the 48 children receiving placebo (2%) was judged to be a drug success.  Seventy-nine percent of the 94 children receiving methylphenidate were judged to be drug success at the end of the 16-week trial period.  A wide range of dosage (0.2 to 1.9 mg/kg) was required for optimum drug effects.  Drug failures were due either to appearance of withdrawn, apathetic behavior in the child (6 cases) or lack of evidence of drug effectiveness (14 cases).  Overweight children did not respond well to medication even though milligram per kilogram dosage was equivalent to that in children who were categorized as drug successes.  The most dramatic successes were seen in children who might be characterized as exhibiting \"developmental hyperactivity,\" i.e., hyperactivity in the absence of other evidence of neurological or emotional disturbances.  We conclude that a trial of stimulant drugs is clearly indicated in hyperactive children manifesting significant impairment of educational and social progress.  Dosage adjustment and judgement of drug success or failure require regular reports from teachers and parents.", "contents": "Observations on effects of a central stimulant drug (methylphenidate) in children with hyperactive behavior. Ninety-eight hyperactive children, ages 6 to 12 years, have been treated with methylphenidate or placebo under double-blind conditions.  Only one of the 48 children receiving placebo (2%) was judged to be a drug success.  Seventy-nine percent of the 94 children receiving methylphenidate were judged to be drug success at the end of the 16-week trial period.  A wide range of dosage (0.2 to 1.9 mg/kg) was required for optimum drug effects.  Drug failures were due either to appearance of withdrawn, apathetic behavior in the child (6 cases) or lack of evidence of drug effectiveness (14 cases).  Overweight children did not respond well to medication even though milligram per kilogram dosage was equivalent to that in children who were categorized as drug successes.  The most dramatic successes were seen in children who might be characterized as exhibiting \"developmental hyperactivity,\" i.e., hyperactivity in the absence of other evidence of neurological or emotional disturbances.  We conclude that a trial of stimulant drugs is clearly indicated in hyperactive children manifesting significant impairment of educational and social progress.  Dosage adjustment and judgement of drug success or failure require regular reports from teachers and parents."}
{"id": "PMID:1093117", "title": "[Is talc a carcinogen? Review of current data].", "content": "The authors review the literature concerned with the carcinogenic hazards of a long term exposure to talc. The epidemiological and experimental data are controversial, but, however, seem to be sufficient to draw attention to, and to introduce special precautions for occupationally exposed individuals. Large experimental and epidemiological studies should be undertaken.", "contents": "[Is talc a carcinogen? Review of current data]. The authors review the literature concerned with the carcinogenic hazards of a long term exposure to talc. The epidemiological and experimental data are controversial, but, however, seem to be sufficient to draw attention to, and to introduce special precautions for occupationally exposed individuals. Large experimental and epidemiological studies should be undertaken."}
{"id": "PMID:1093118", "title": "[Cardiac hemochromatosis. Anatomic, clinical and hemodynamical data].", "content": "The cardiovascular manifestations of idiopathic familial haemochromatosis (I.H.) should be well known. Of 25 patients seen for the most part in a Nutrition-Endocrinology Service, 10 suffered a purely cardiological problem at some point in their course.14 out of 18 patients had definite haemodynamic abnormalities. The discovery of cardiac haemochromatosis necessitates: a) depletive therapy by venesection which in certain cases is associated with the returm to normal of clinical and haemodynamic abnormalities (8-18-25). b) careful investigation of the family which, when positive, gives evidence of the primary nature of the iron excess. In members of the family suffering from I.H., early preventive therapy by vensections avoids the development of cardiovascular complications which are responsible for approximately 10 to 15 p.cent of causes of death in association with I.H.", "contents": "[Cardiac hemochromatosis. Anatomic, clinical and hemodynamical data]. The cardiovascular manifestations of idiopathic familial haemochromatosis (I.H.) should be well known. Of 25 patients seen for the most part in a Nutrition-Endocrinology Service, 10 suffered a purely cardiological problem at some point in their course.14 out of 18 patients had definite haemodynamic abnormalities. The discovery of cardiac haemochromatosis necessitates: a) depletive therapy by venesection which in certain cases is associated with the returm to normal of clinical and haemodynamic abnormalities (8-18-25). b) careful investigation of the family which, when positive, gives evidence of the primary nature of the iron excess. In members of the family suffering from I.H., early preventive therapy by vensections avoids the development of cardiovascular complications which are responsible for approximately 10 to 15 p.cent of causes of death in association with I.H."}
{"id": "PMID:1093120", "title": "[Circulatory survival of irreversible comas].", "content": "On the basis of a series of 53 cases of irreversible coma maintained in circulatory survival with the aim of removing the kidneys, the authors discuss the mode of treatment, with particular reference to the intravenous fluids used and the use of medications influencing the circulation. Fluid and electrolytes given must be adjusted hourly to ensure the exact replacement of urinary losses. Isoprotenerol is the only medication usually necessary. In the event of circulatory insufficiency, which is difficult to foresee and hence prevent, immediate volume expansion in a  short a time as possible and isoprotenerol most frequently correct the situation (14 out of 17 cases). Thus effective circulation may be maintained until the kidneys are removed (48 out of 53 cases). 92 p.cent of the grafted kidneys functioned from the first day onwards.", "contents": "[Circulatory survival of irreversible comas]. On the basis of a series of 53 cases of irreversible coma maintained in circulatory survival with the aim of removing the kidneys, the authors discuss the mode of treatment, with particular reference to the intravenous fluids used and the use of medications influencing the circulation. Fluid and electrolytes given must be adjusted hourly to ensure the exact replacement of urinary losses. Isoprotenerol is the only medication usually necessary. In the event of circulatory insufficiency, which is difficult to foresee and hence prevent, immediate volume expansion in a  short a time as possible and isoprotenerol most frequently correct the situation (14 out of 17 cases). Thus effective circulation may be maintained until the kidneys are removed (48 out of 53 cases). 92 p.cent of the grafted kidneys functioned from the first day onwards."}
{"id": "PMID:1093127", "title": "[Pneumocystis carinii pneumonia after kidney transplantation. Early diagnosis by distal bronchial brushing].", "content": "Distal bronchial brushing is one of the methods used in the in vivo indentification of Pneumocystis carinii, a parasite associated with severe pneumonia, the development of which is induced by immuno-depression, particularly after renal transplantation. In three patients the pathogenic agent was demonstrated in alveolar material obtained in this way. The technique is simple and risk-free. It may be used early and repeatedly.", "contents": "[Pneumocystis carinii pneumonia after kidney transplantation. Early diagnosis by distal bronchial brushing]. Distal bronchial brushing is one of the methods used in the in vivo indentification of Pneumocystis carinii, a parasite associated with severe pneumonia, the development of which is induced by immuno-depression, particularly after renal transplantation. In three patients the pathogenic agent was demonstrated in alveolar material obtained in this way. The technique is simple and risk-free. It may be used early and repeatedly."}
{"id": "PMID:1093136", "title": "Binding of lactose repressor to poly d(A-T) : OD AND CD melting of the complex.", "content": "The binding of lactose repressor to poly d(A-T) at low ionic strength has been investigated by heat denaturation. The poly d(A-T) melting is monitored by optical density and the protein melting by circular dichroism. From the modification of the poly d(A-T) melting curve we estimate a maximum binding ratio of about one tetrameric repressor to about 20 basic pairs. The repressor melting can be interpreted as a global shift from a to b structure of about 25 residues per subunit. The melting curves of poly d(A-T) and repressor have not a shape easy to interpret; nevertheless both show a cooperative transition in the same temperature range where we can evaluate that about 3.8 aminoacid residues shift from a to b structure when 1 bases pair melt.", "contents": "Binding of lactose repressor to poly d(A-T) : OD AND CD melting of the complex. The binding of lactose repressor to poly d(A-T) at low ionic strength has been investigated by heat denaturation. The poly d(A-T) melting is monitored by optical density and the protein melting by circular dichroism. From the modification of the poly d(A-T) melting curve we estimate a maximum binding ratio of about one tetrameric repressor to about 20 basic pairs. The repressor melting can be interpreted as a global shift from a to b structure of about 25 residues per subunit. The melting curves of poly d(A-T) and repressor have not a shape easy to interpret; nevertheless both show a cooperative transition in the same temperature range where we can evaluate that about 3.8 aminoacid residues shift from a to b structure when 1 bases pair melt."}
{"id": "PMID:1093137", "title": "On the statistical significance of primary structural features found in DNA-protein interaction sites.", "content": "Probabilities of occurrence for a number of the symmetries and other sequence regularities found in DNA-protein interaction site sequences have been calculated for segments of random DNA sequence. Results show that many of the symmetrical and repetitive features seen in these interaction sites are likely to have occured by chance. Other features are so unlikely to have occurred by chance that they are probably involved in the DNA-protein interaction processes.", "contents": "On the statistical significance of primary structural features found in DNA-protein interaction sites. Probabilities of occurrence for a number of the symmetries and other sequence regularities found in DNA-protein interaction site sequences have been calculated for segments of random DNA sequence. Results show that many of the symmetrical and repetitive features seen in these interaction sites are likely to have occured by chance. Other features are so unlikely to have occurred by chance that they are probably involved in the DNA-protein interaction processes."}
{"id": "PMID:1093138", "title": "The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique.", "content": "The preparation of phage lambda DNA infecting E. coli K 12 with cationic detergent is described. This DNA infects E. coli spheroblasts with the same efficiency as DNA prepared by phenol methods.", "contents": "The biological activity of bacteriophage DNA, prepared by the cationic detergent dilution technique. The preparation of phage lambda DNA infecting E. coli K 12 with cationic detergent is described. This DNA infects E. coli spheroblasts with the same efficiency as DNA prepared by phenol methods."}
{"id": "PMID:1093139", "title": "Photo-incuced cross-linkage of gene-5 protein and bacteriophage fd DNA+.", "content": "The gene 5 protein, coded for by the bacteriophage fd, forms a complex with single stranded fd-DNA such that one gene 5 protein monomer interacts with four bases. Exposure of this complex to ultraviolet light results in the formation of covalent bonds between 25-30% of the gene 5 protein monomers which are bound to the DNA. In contrast, when the intact fd virion, which is a complex of coat protein and DNA, was exposed to ultraviolet irradiation, no detectable protein DNA cross-links were found.", "contents": "Photo-incuced cross-linkage of gene-5 protein and bacteriophage fd DNA+. The gene 5 protein, coded for by the bacteriophage fd, forms a complex with single stranded fd-DNA such that one gene 5 protein monomer interacts with four bases. Exposure of this complex to ultraviolet light results in the formation of covalent bonds between 25-30% of the gene 5 protein monomers which are bound to the DNA. In contrast, when the intact fd virion, which is a complex of coat protein and DNA, was exposed to ultraviolet irradiation, no detectable protein DNA cross-links were found."}
{"id": "PMID:1093140", "title": "The secondary structure of E. coli ribosomes and ribosomal RNA's: a spectrophotometric approach.", "content": "The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs derived from model polyribonucleotides. The coefficients of these linear combinations were used to calculate the fractions of A - U and G - C base pairs in the samples. It was found that the helical content of the RNA's inside the subunits is smaller than that of the isolated RNA's, thus suggesting that proteins may affect the secondary structure of RNA in the ribosome.", "contents": "The secondary structure of E. coli ribosomes and ribosomal RNA's: a spectrophotometric approach. The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs derived from model polyribonucleotides. The coefficients of these linear combinations were used to calculate the fractions of A - U and G - C base pairs in the samples. It was found that the helical content of the RNA's inside the subunits is smaller than that of the isolated RNA's, thus suggesting that proteins may affect the secondary structure of RNA in the ribosome."}
{"id": "PMID:1093141", "title": "(dA-dT) dependent inactivation of the DNA template properties by interaction with netropsin and distamycin A.", "content": "The inhibitory effect of the polypeptide antibiotics netropsin and distamycin A on DNA dependent nucleic acid synthesis has been shown to be related to the base composition of the template DNA. A number of natural DNA's of quite different dA-dT content as well as poly (dI-dC)-poly (dI-dC), poly (dA-dT)-poly (dA-dT), poly (dA) - poly (dT) and poly (dG) - poly (dC) has been studied as templates in DNA and in part in RNA polymerase reaction. The highest binding efficiency of netropsin existing for (dA-dT) - containing DNA polymers and the less pronounced interaction with the (dI-dC)-containing polymer shown by the melting and CD spectrral behaviour of the complexes are entirely reflected in the template inactivation. The same is evident for distamycin A. However, in contrast to netropsin the antibiotic distamycin A exhibits some binding tendency to poly (dG) - poly (dC). Binding effects of a netropsin derivative to DNA and (dA-dT) -containing polymers suggest the importance of hydrogen bonds of the peptide groups in the complex formation.", "contents": "(dA-dT) dependent inactivation of the DNA template properties by interaction with netropsin and distamycin A. The inhibitory effect of the polypeptide antibiotics netropsin and distamycin A on DNA dependent nucleic acid synthesis has been shown to be related to the base composition of the template DNA. A number of natural DNA's of quite different dA-dT content as well as poly (dI-dC)-poly (dI-dC), poly (dA-dT)-poly (dA-dT), poly (dA) - poly (dT) and poly (dG) - poly (dC) has been studied as templates in DNA and in part in RNA polymerase reaction. The highest binding efficiency of netropsin existing for (dA-dT) - containing DNA polymers and the less pronounced interaction with the (dI-dC)-containing polymer shown by the melting and CD spectrral behaviour of the complexes are entirely reflected in the template inactivation. The same is evident for distamycin A. However, in contrast to netropsin the antibiotic distamycin A exhibits some binding tendency to poly (dG) - poly (dC). Binding effects of a netropsin derivative to DNA and (dA-dT) -containing polymers suggest the importance of hydrogen bonds of the peptide groups in the complex formation."}
{"id": "PMID:1093142", "title": "Accessibility of chromatin to DNA polymerase I and location of the F1 histone.", "content": "The effect of prebound poly-L-lysine upon the template activity of DNA, chromatin and F1 histone-depleted chromatin for E. coli DNA polymerase I has been investigated. Measurements have been made in the absence and presence of 0.1M NaCl. From the results we conclude that only ca 60% of the polylysine-accessible DNA, i.e. 22% of the total DNA of the chromatin, is accessible to the DNA polymerase I and that ca. 30% of the polylysine-accessible DNA, i.e. 11% of the total DNA, is associated with the F1 histone.", "contents": "Accessibility of chromatin to DNA polymerase I and location of the F1 histone. The effect of prebound poly-L-lysine upon the template activity of DNA, chromatin and F1 histone-depleted chromatin for E. coli DNA polymerase I has been investigated. Measurements have been made in the absence and presence of 0.1M NaCl. From the results we conclude that only ca 60% of the polylysine-accessible DNA, i.e. 22% of the total DNA of the chromatin, is accessible to the DNA polymerase I and that ca. 30% of the polylysine-accessible DNA, i.e. 11% of the total DNA, is associated with the F1 histone."}
{"id": "PMID:1093143", "title": "Enzymatic multiplication of a chemically synthesized DNA fragment.", "content": "A synthetic DNA fragment of 19 residues was enlarged by the enzymatic addition of deoxyadenylate residues to its 3'-end with calf thymus terminal deoxynucleotidyl transferase. The 3'-terminus of this elongated DNA strand was blocked with 2', 3'-dideoxyadenylate to prevent hydrolysis by the 3'-exonuclease function of E. coli DNA polymerase I. This elongated and 3'-blocked fragment was annealed to an oligomeric primer and used as a template for the synthesis of a complementary copy of the synthetic 19-mer. The product of such a repair synthesis was separated by gel filtration and analyzed by nearest neighbor techniques. All template strands were copied with complete repair in over 90% of the chains. Facile recovery of the elongated template by virtue of its size permitted repetition of the copy process, thus allowing accumulation of the desired strand.", "contents": "Enzymatic multiplication of a chemically synthesized DNA fragment. A synthetic DNA fragment of 19 residues was enlarged by the enzymatic addition of deoxyadenylate residues to its 3'-end with calf thymus terminal deoxynucleotidyl transferase. The 3'-terminus of this elongated DNA strand was blocked with 2', 3'-dideoxyadenylate to prevent hydrolysis by the 3'-exonuclease function of E. coli DNA polymerase I. This elongated and 3'-blocked fragment was annealed to an oligomeric primer and used as a template for the synthesis of a complementary copy of the synthetic 19-mer. The product of such a repair synthesis was separated by gel filtration and analyzed by nearest neighbor techniques. All template strands were copied with complete repair in over 90% of the chains. Facile recovery of the elongated template by virtue of its size permitted repetition of the copy process, thus allowing accumulation of the desired strand."}
{"id": "PMID:1093144", "title": "Formylatable methionine transfer RNA from Mycoplasma: purification and comparison of partial nucleotide sequences with those of other prokaryotic initiator tRNAs.", "content": "The major species of the formylatable methionine tRNA from Mycoplasma mycoides var capri has been purified. The 5'- and 3'-terminal sequences of the purified tRNA are pC-G- and C-A-A-C-C-AOH, respectively. Thus, this tRNA also contains the unique structural feature found in two other prokaryotic initiator tRNAs in that the first nucleotide at the 5'-end cannot form a Watson-Crick type of base-pair to the fifth nucleotide from the 3'-end. The Mycoplasma tRNA does not contain ribothymidine; however, a specific uridine residue in the sequence G-U-psi-C-G- can be enzymatically methylated by E. coli extracts to yield G-T-psi-C-G. Since ribothymidine is absent in crude tRNA from this strain of  Mycoplasma, the absence of T is probably due to the lack of a U yields T modifying enzyme.", "contents": "Formylatable methionine transfer RNA from Mycoplasma: purification and comparison of partial nucleotide sequences with those of other prokaryotic initiator tRNAs. The major species of the formylatable methionine tRNA from Mycoplasma mycoides var capri has been purified. The 5'- and 3'-terminal sequences of the purified tRNA are pC-G- and C-A-A-C-C-AOH, respectively. Thus, this tRNA also contains the unique structural feature found in two other prokaryotic initiator tRNAs in that the first nucleotide at the 5'-end cannot form a Watson-Crick type of base-pair to the fifth nucleotide from the 3'-end. The Mycoplasma tRNA does not contain ribothymidine; however, a specific uridine residue in the sequence G-U-psi-C-G- can be enzymatically methylated by E. coli extracts to yield G-T-psi-C-G. Since ribothymidine is absent in crude tRNA from this strain of  Mycoplasma, the absence of T is probably due to the lack of a U yields T modifying enzyme."}
{"id": "PMID:1093162", "title": "Replication of colicin E1 plasmid DNA added to cell extracts.", "content": "Closed-circular DNA of colicin E1 plasmid can undergo a round of semiconservative replication when added to an extract of Escherichia coli. Extracts of cells that do not carry the plasmid are able to perform complete replication of the plasmid. Replication requires de novo RNA synthesis but not protein synthesis.", "contents": "Replication of colicin E1 plasmid DNA added to cell extracts. Closed-circular DNA of colicin E1 plasmid can undergo a round of semiconservative replication when added to an extract of Escherichia coli. Extracts of cells that do not carry the plasmid are able to perform complete replication of the plasmid. Replication requires de novo RNA synthesis but not protein synthesis."}
{"id": "PMID:1093161", "title": "[The effect of sympatholytics and adrenergic receptor blockade on the histophysiology of the insular apparatus of the pancreas and insulin sensitivity of white rats].", "content": "Experiments were conducted on 200 male albino rats.  It was shown that prolonged administration of sympatholytics (ornide or actadine) and also of a blocker of the alpha-adrenorecptors with phentholamine or of beta-adrenoreceptors with inderal led to the accumulation of zinc in the cytoplasm of beta-cells, reduction of the content in them of specific aldehyde-fuchsinophilic granularity, neoformation of the islands of Langerhans and to increase in the animal sensitivity to the exogenous insulin.", "contents": "[The effect of sympatholytics and adrenergic receptor blockade on the histophysiology of the insular apparatus of the pancreas and insulin sensitivity of white rats]. Experiments were conducted on 200 male albino rats.  It was shown that prolonged administration of sympatholytics (ornide or actadine) and also of a blocker of the alpha-adrenorecptors with phentholamine or of beta-adrenoreceptors with inderal led to the accumulation of zinc in the cytoplasm of beta-cells, reduction of the content in them of specific aldehyde-fuchsinophilic granularity, neoformation of the islands of Langerhans and to increase in the animal sensitivity to the exogenous insulin."}
{"id": "PMID:1093163", "title": "N-formylmethionyl peptides as chemoattractants for leucocytes.", "content": "Leucocytes such as neutrophils are attracted by N-formylmethionine, but not by methionine. Di- and tripeptides containing formylmethionine are strong attractants for both neutrophils and macrophages, whereas the corresponding nonacylated compounds are not chemotactic. The formylated peptides may be related to an incompletely characterized chemotactic material normally produced by bacteria which attract the same animal cells.", "contents": "N-formylmethionyl peptides as chemoattractants for leucocytes. Leucocytes such as neutrophils are attracted by N-formylmethionine, but not by methionine. Di- and tripeptides containing formylmethionine are strong attractants for both neutrophils and macrophages, whereas the corresponding nonacylated compounds are not chemotactic. The formylated peptides may be related to an incompletely characterized chemotactic material normally produced by bacteria which attract the same animal cells."}
{"id": "PMID:1093160", "title": "[Alterations in the histostructure of the pancreas in experimental diabetes caused by quinoline derivatives].", "content": "Administration of chinoline derivatives, with a capacity to form stable complexes with metals (zinc?), was accompanied by a selective damage of the pancreatic islands with development of diabetes mellitus. Changes in the histostructure of the tissue caused by chinoline compounds of various chemical structure were unitypical. Their severity chiefly depended on the severity and duration of diabetes. Diabetes under study arising after the administration of chinoline derivatives were good models for detailed ascertaining of histostructure of the islet tissue and of the pancreatic duct in diabetes.", "contents": "[Alterations in the histostructure of the pancreas in experimental diabetes caused by quinoline derivatives]. Administration of chinoline derivatives, with a capacity to form stable complexes with metals (zinc?), was accompanied by a selective damage of the pancreatic islands with development of diabetes mellitus. Changes in the histostructure of the tissue caused by chinoline compounds of various chemical structure were unitypical. Their severity chiefly depended on the severity and duration of diabetes. Diabetes under study arising after the administration of chinoline derivatives were good models for detailed ascertaining of histostructure of the islet tissue and of the pancreatic duct in diabetes."}
{"id": "PMID:1093164", "title": "Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients.", "content": "The transfer ribonucleic acids of Escherichia coli bind to unsubstituted Sepharose in the presence of high concentrations of ammonium sulfate at pH 4.5. Transfer RNA species are eluted individually from the Sepharose by a gradient from high to low concentrations of ammonium sulfate; leucine tRNA is fractionated into five isoaccepting species. The order of elution of these isoaccepting species differs from that seen with reverse phase chromatography. By means of only these two procedures, one isoaccepting species of leucine tRNA can be purified to apparent homogeneity. Isoaccepting tRNA species for 9 amino acids have been resolved. This established the general utility of this chromatographic system for the separation and purification of specific isoaccepting transfer RNAs.", "contents": "Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients. The transfer ribonucleic acids of Escherichia coli bind to unsubstituted Sepharose in the presence of high concentrations of ammonium sulfate at pH 4.5. Transfer RNA species are eluted individually from the Sepharose by a gradient from high to low concentrations of ammonium sulfate; leucine tRNA is fractionated into five isoaccepting species. The order of elution of these isoaccepting species differs from that seen with reverse phase chromatography. By means of only these two procedures, one isoaccepting species of leucine tRNA can be purified to apparent homogeneity. Isoaccepting tRNA species for 9 amino acids have been resolved. This established the general utility of this chromatographic system for the separation and purification of specific isoaccepting transfer RNAs."}
{"id": "PMID:1093165", "title": "Chromatin directed transcription of 5S and tRNA genes.", "content": "Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei.", "contents": "Chromatin directed transcription of 5S and tRNA genes. Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei."}
{"id": "PMID:1093166", "title": "Biogenesis of membrane lipids: mutants of Escherichia coli with temperature-sensitive phosphatidylserine decarboxylase.", "content": "Phosphatidylserine decarboxylase catalyzes the last step in the pathway leading to phosphatidylethanolamine, the principal membrane lipid of E. coli. Mutants of E. coli have now been isolated in which this enzyme is theramolabile. The structural gene for phosphatidylserine decarboxylase (psd gene) is closely linked to the pur A locus at about 83 min on the standard map of the E. coli chromosome. When a mutant with thermolabile decarboxylase is incubated at 42 degrees, growth ceases, but only after a substantial fraction (20-40%) of the total phospholipid of the cell has been replaced by phosphatidylserine. Examination of such mutants with altered content of phospholipids may shed light on the role of specific phospholipids in membrane function.", "contents": "Biogenesis of membrane lipids: mutants of Escherichia coli with temperature-sensitive phosphatidylserine decarboxylase. Phosphatidylserine decarboxylase catalyzes the last step in the pathway leading to phosphatidylethanolamine, the principal membrane lipid of E. coli. Mutants of E. coli have now been isolated in which this enzyme is theramolabile. The structural gene for phosphatidylserine decarboxylase (psd gene) is closely linked to the pur A locus at about 83 min on the standard map of the E. coli chromosome. When a mutant with thermolabile decarboxylase is incubated at 42 degrees, growth ceases, but only after a substantial fraction (20-40%) of the total phospholipid of the cell has been replaced by phosphatidylserine. Examination of such mutants with altered content of phospholipids may shed light on the role of specific phospholipids in membrane function."}
{"id": "PMID:1093167", "title": "Cell cycle-dependent induction of mutations along a yeast chromosome.", "content": "The relation between DNA replication and the action of the mutagen N-methyl-N'-nitro-N-nitroso-guanidine has been studied in Saccharomyces cerevisiae. The frequenceis of reversion to prototrophy of six auxotrophic markers located along one arm of chromosome VII were examined as a function of the vegetative cell cycle. Exponentially growing cells were treated with nitrosoguanidine and then separated by zonal rotor centrifugation into fractions equivalent to stages in the cell cycle. The frequency of reversion for five of the six markers is greatest during the period of DNA replication. Each marker has a single point of maximum reversion, approximately 10-fold greater than the frequency observed at other points in the cell cycle. For any one marker the effect of nitrosoguanidine is restricted to an interval shorter than the period of DNA replication. The two markers most distant from each other, ade5 and leul, both have their highest reversion frequency early during DNA replication. The peak reversion frequency for lys5 is somewhat later, while the peaks for tyr3 and trp5 occur near the end of DNA replication. The results indicate that nitrosoguanidine acts primarily during DNA replication and that different markers appear to be affected at different intervals during the DNA biosynthetic period. If nitrosoguanidine does act at the growing point of DNA replication, these observations indicate that the initiation of DNA replication occurs at specific times during the period of DNA synthesis and at specific initiation sites. Further, there must be more than one point of initiation of DNA replication on one arm of chromosome VII.", "contents": "Cell cycle-dependent induction of mutations along a yeast chromosome. The relation between DNA replication and the action of the mutagen N-methyl-N'-nitro-N-nitroso-guanidine has been studied in Saccharomyces cerevisiae. The frequenceis of reversion to prototrophy of six auxotrophic markers located along one arm of chromosome VII were examined as a function of the vegetative cell cycle. Exponentially growing cells were treated with nitrosoguanidine and then separated by zonal rotor centrifugation into fractions equivalent to stages in the cell cycle. The frequency of reversion for five of the six markers is greatest during the period of DNA replication. Each marker has a single point of maximum reversion, approximately 10-fold greater than the frequency observed at other points in the cell cycle. For any one marker the effect of nitrosoguanidine is restricted to an interval shorter than the period of DNA replication. The two markers most distant from each other, ade5 and leul, both have their highest reversion frequency early during DNA replication. The peak reversion frequency for lys5 is somewhat later, while the peaks for tyr3 and trp5 occur near the end of DNA replication. The results indicate that nitrosoguanidine acts primarily during DNA replication and that different markers appear to be affected at different intervals during the DNA biosynthetic period. If nitrosoguanidine does act at the growing point of DNA replication, these observations indicate that the initiation of DNA replication occurs at specific times during the period of DNA synthesis and at specific initiation sites. Further, there must be more than one point of initiation of DNA replication on one arm of chromosome VII."}
{"id": "PMID:1093168", "title": "Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.", "content": "Escherichia coli RNA polymerase (EC 2.7.7.6), bound in a tight complex at an early T7 promoter, protects 41 to 43 base pairs of DNA from digestion by DNase. I. The protected DNA fragment contains both the binding site for RNA polymerase and the mRNA initiation point for the promoter. The sequence of the DNA fragment and the sequence of the mRNA that it codes for are presented here. A seven-base-pair sequence, apparently common to all promoters, is implicated in the formation of a tight binary complex with RNA polymerase.", "contents": "Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Escherichia coli RNA polymerase (EC 2.7.7.6), bound in a tight complex at an early T7 promoter, protects 41 to 43 base pairs of DNA from digestion by DNase. I. The protected DNA fragment contains both the binding site for RNA polymerase and the mRNA initiation point for the promoter. The sequence of the DNA fragment and the sequence of the mRNA that it codes for are presented here. A seven-base-pair sequence, apparently common to all promoters, is implicated in the formation of a tight binary complex with RNA polymerase."}
{"id": "PMID:1093169", "title": "The relationship between enzyme activity, cell geometry, and fitness in Saccharomyces cerevisiae.", "content": "The relationship between enzyme activity, cell geometry, and the ploidy levels has been investigated in Saccharomyces cerevisiae. Diploid cells have 1.57 times the volume of haploid cells under nonlimiting growth conditions (minimal medium). However, when diploid cells are grown under conditions of carbon limitation, they have the same volume as haploid cells. Thus, by altering the environmental conditions, cell size can be varied independently of the degree of ploidy. The results indicate that the basic biochemical parameters of the cell are primarily determined by cell geometry rather than ploidy level. RNA content, protein content, and ornithine transcarbamylase (carbamoylphosphate: L-ornithine carbamoyltransferase, EC 2.1.3.3), tryptophan synthetase [L-serine hydro-lyase (adding indole), EC 4.2.1.20], and invertase (alpha-D-glucoside glucohydrolase, Ec 3.2.1.20) activity are related to cell volume, whereas acid phosphatase (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2) activity, a cell surface enzyme, is related to the surface area of the cells. Fitness is determined by the activity of certain cell surface enzymes, such as acid phosphatase, diploids would be expected to have a lower fitness than haploids because of the lower surface area/volume ratio. However, when fitness is determined by the activity of an internal enzyme, diploids would be expected to have the same fitness as haploids. Results from competition experiments between haploids and diploids are consistent with these predictions. The significance of these results to the evolution of diploidy as the predominant phase of the life cycle of higher plants and animals is discussed.", "contents": "The relationship between enzyme activity, cell geometry, and fitness in Saccharomyces cerevisiae. The relationship between enzyme activity, cell geometry, and the ploidy levels has been investigated in Saccharomyces cerevisiae. Diploid cells have 1.57 times the volume of haploid cells under nonlimiting growth conditions (minimal medium). However, when diploid cells are grown under conditions of carbon limitation, they have the same volume as haploid cells. Thus, by altering the environmental conditions, cell size can be varied independently of the degree of ploidy. The results indicate that the basic biochemical parameters of the cell are primarily determined by cell geometry rather than ploidy level. RNA content, protein content, and ornithine transcarbamylase (carbamoylphosphate: L-ornithine carbamoyltransferase, EC 2.1.3.3), tryptophan synthetase [L-serine hydro-lyase (adding indole), EC 4.2.1.20], and invertase (alpha-D-glucoside glucohydrolase, Ec 3.2.1.20) activity are related to cell volume, whereas acid phosphatase (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2) activity, a cell surface enzyme, is related to the surface area of the cells. Fitness is determined by the activity of certain cell surface enzymes, such as acid phosphatase, diploids would be expected to have a lower fitness than haploids because of the lower surface area/volume ratio. However, when fitness is determined by the activity of an internal enzyme, diploids would be expected to have the same fitness as haploids. Results from competition experiments between haploids and diploids are consistent with these predictions. The significance of these results to the evolution of diploidy as the predominant phase of the life cycle of higher plants and animals is discussed."}
{"id": "PMID:1093170", "title": "Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form.", "content": "The intracellular localization of phytochrome was seen in dark-grown oat (Avena sativa L., cv. Garry) and rice (Oryza sativa L., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. Phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. Within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absorbing form, becomes associated with discrete regions of the cell. These regions do not appear to be nuclei, plastids, or mitochondria. After phototransformation back to the red-absorbing form originally present, phytochrome slowly resumes its general distribution. It is possible that this discrete localization of the far-red-absorbing form of phytochrome represents a physiologically significant binding with a receptor site in the cell.", "contents": "Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. The intracellular localization of phytochrome was seen in dark-grown oat (Avena sativa L., cv. Garry) and rice (Oryza sativa L., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. Phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. Within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absorbing form, becomes associated with discrete regions of the cell. These regions do not appear to be nuclei, plastids, or mitochondria. After phototransformation back to the red-absorbing form originally present, phytochrome slowly resumes its general distribution. It is possible that this discrete localization of the far-red-absorbing form of phytochrome represents a physiologically significant binding with a receptor site in the cell."}
{"id": "PMID:1093171", "title": "Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.", "content": "The lac genes were fused to the ara promoter by means of phage phi 80 translocations of the lac and ara genes to att80. Homology for a crossover between the nonhomologous ara and lac operons was provided by mu insertions. Selection for recombinants within the mu insertions generated strains that had the ara promoter on one side of a mu insertion and the lac genes on the other side. ara-lac fusions were obtained from these strains by deleting the mu insertion. These fusions extend the techniques available for studies on the lac operon to studies on the ara operon. It should be possible to fuse other operons by this method.", "contents": "Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions. The lac genes were fused to the ara promoter by means of phage phi 80 translocations of the lac and ara genes to att80. Homology for a crossover between the nonhomologous ara and lac operons was provided by mu insertions. Selection for recombinants within the mu insertions generated strains that had the ara promoter on one side of a mu insertion and the lac genes on the other side. ara-lac fusions were obtained from these strains by deleting the mu insertion. These fusions extend the techniques available for studies on the lac operon to studies on the ara operon. It should be possible to fuse other operons by this method."}
{"id": "PMID:1093172", "title": "Replicative intermediates of colicin E1 plasmid DNA in minicells.", "content": "Pulse-labeled colicin E1 plasmid (Col E1) DNA in minicells was examined to characterize replicating molecules. Replication of Col E1 DNA principally occurred in covalently closed molecules and involved the synthesis of variable length single-stranded DNA fragments that were dissociable from the template DNA and ultimately incorporated into the completely replicated molecules. RNA was found to be associated with some of these newly synthesized fragments. Replicating molecules containing different size displacement loops were observed by electron microscopy.", "contents": "Replicative intermediates of colicin E1 plasmid DNA in minicells. Pulse-labeled colicin E1 plasmid (Col E1) DNA in minicells was examined to characterize replicating molecules. Replication of Col E1 DNA principally occurred in covalently closed molecules and involved the synthesis of variable length single-stranded DNA fragments that were dissociable from the template DNA and ultimately incorporated into the completely replicated molecules. RNA was found to be associated with some of these newly synthesized fragments. Replicating molecules containing different size displacement loops were observed by electron microscopy."}
{"id": "PMID:1093173", "title": "Regulation of RNA polymerase synthesis in Escherichia coli: a mutant unable to synthesize the enzyme at 43 degrees.", "content": "We report the isolation of a mutant of E. coli in which the capacity to synthesize RNA polymerase (EC 2.7.7.6) (the beta and beta' subunits) is rapidly lost at 43 degrees. The mutation has no effect on the stability or activity of the polymerase itself. The mutation is recessive and is closely linked to the rif locus (the structural gene for the beta subunit). Using strains carrying the mutation, we have shown that polymerase is present in excess in rapidly growing E. coli cells.", "contents": "Regulation of RNA polymerase synthesis in Escherichia coli: a mutant unable to synthesize the enzyme at 43 degrees. We report the isolation of a mutant of E. coli in which the capacity to synthesize RNA polymerase (EC 2.7.7.6) (the beta and beta' subunits) is rapidly lost at 43 degrees. The mutation has no effect on the stability or activity of the polymerase itself. The mutation is recessive and is closely linked to the rif locus (the structural gene for the beta subunit). Using strains carrying the mutation, we have shown that polymerase is present in excess in rapidly growing E. coli cells."}
{"id": "PMID:1093174", "title": "Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.", "content": "Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.", "contents": "Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro. Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically."}
{"id": "PMID:1093175", "title": "Molecular basis of beta-galactosidase alpha-complementation.", "content": "In previous studies, a cyanogen bromide peptide derived from amino-acid residues 3-92 of beta-galactosidase (EC 3.2.1.23; beta-D-galactoside galactohydrolase) was shown to have alpha-donor activity in intracistronic alpha-complementation. We have now isolated the defective beta-galactosidase alpha-acceptor protein from the deletion mutant strain M15 of Escherichia coli and find that it lacks residues 11-41 of betal-galactosidase. This is demonstrated by the isolation and sequence determination of a cyanogen bromide peptide from the M15 protein, which is identical to the corresponding peptide from beta-galactosidase except for the missing amino acids. We conclude that the alpha-donor peptide restores the region missing in the M15 protein.", "contents": "Molecular basis of beta-galactosidase alpha-complementation. In previous studies, a cyanogen bromide peptide derived from amino-acid residues 3-92 of beta-galactosidase (EC 3.2.1.23; beta-D-galactoside galactohydrolase) was shown to have alpha-donor activity in intracistronic alpha-complementation. We have now isolated the defective beta-galactosidase alpha-acceptor protein from the deletion mutant strain M15 of Escherichia coli and find that it lacks residues 11-41 of betal-galactosidase. This is demonstrated by the isolation and sequence determination of a cyanogen bromide peptide from the M15 protein, which is identical to the corresponding peptide from beta-galactosidase except for the missing amino acids. We conclude that the alpha-donor peptide restores the region missing in the M15 protein."}
{"id": "PMID:1093176", "title": "New cell surface antigens in rat defined by tumors of the nervous system.", "content": "Tumors of the central and peripheral nervous system were induced in rats with ethylnitrosourea. Many of these tumors were transplanted in syngeneic recipients, and several cell lines were derived from them. An antiserum raised against one such cell line in C3H mice defined two cell surface antigens in cytotoxicity tests. One, the common antigen, was present on rat brain and embryonic tissues and was present in large amounts on most tumors or cell lines from the nervous system. Fibroblastic cell lines had smaller amounts of this antigen, which also could be detected by immunofluorescence. The other, restricted antigen was not detected on normal or other, restricted antigen was not detected on normal or embryonic tissues. It was present on six tumors from the nervous system, on one glial cell line, and on a Schwann-cell line RN22. In addition, it was present on four out of eleven cloned cell lines isolated from rat tumors at the Salk Institute. Two of the positive clonal lines had been shown to have properties unique to neuronal cells. The restricted antigen was therefore expressed on the cell surface of some, but not all, glial, Schwann, and neuronal neoplastic cells.", "contents": "New cell surface antigens in rat defined by tumors of the nervous system. Tumors of the central and peripheral nervous system were induced in rats with ethylnitrosourea. Many of these tumors were transplanted in syngeneic recipients, and several cell lines were derived from them. An antiserum raised against one such cell line in C3H mice defined two cell surface antigens in cytotoxicity tests. One, the common antigen, was present on rat brain and embryonic tissues and was present in large amounts on most tumors or cell lines from the nervous system. Fibroblastic cell lines had smaller amounts of this antigen, which also could be detected by immunofluorescence. The other, restricted antigen was not detected on normal or other, restricted antigen was not detected on normal or embryonic tissues. It was present on six tumors from the nervous system, on one glial cell line, and on a Schwann-cell line RN22. In addition, it was present on four out of eleven cloned cell lines isolated from rat tumors at the Salk Institute. Two of the positive clonal lines had been shown to have properties unique to neuronal cells. The restricted antigen was therefore expressed on the cell surface of some, but not all, glial, Schwann, and neuronal neoplastic cells."}
{"id": "PMID:1093177", "title": "Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas.", "content": "A discrete population of cells of the endocrine pancreas contains immunoreactive somatostatin as shown by immunofluorescence. These cells are different from those containing glucagon or insulin. This unexpected observation may be of physiopathological significance in the regulatory mechanisms involved in the secretion of glucagon and insulin.", "contents": "Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. A discrete population of cells of the endocrine pancreas contains immunoreactive somatostatin as shown by immunofluorescence. These cells are different from those containing glucagon or insulin. This unexpected observation may be of physiopathological significance in the regulatory mechanisms involved in the secretion of glucagon and insulin."}
{"id": "PMID:1093178", "title": "Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.", "content": "Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.", "contents": "Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments. Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined."}
{"id": "PMID:1093179", "title": "Proposal concerning mechanism of evolution of the genome of Escherichia coli.", "content": "Many pairs of genes whose gene products are functionally related lie either 90 degrees or 180 degrees apart on the circular map of the E. coli chromosome. A mechanism of evolution is proposed that involves two sequential duplications of an ancestral genome, followed by mutation and divergence of function of replicate genes.", "contents": "Proposal concerning mechanism of evolution of the genome of Escherichia coli. Many pairs of genes whose gene products are functionally related lie either 90 degrees or 180 degrees apart on the circular map of the E. coli chromosome. A mechanism of evolution is proposed that involves two sequential duplications of an ancestral genome, followed by mutation and divergence of function of replicate genes."}
{"id": "PMID:1093180", "title": "Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.", "content": "A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their original DNA sequence orientation. The events observed entail nonreciprocal exchange of genetic material, and involve a discrete nucleotide sequences that is duplicated in rotationally symmetrical reverse orientation on plasmid DNA (i.e., inverted repeat; palindrome).", "contents": "Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their original DNA sequence orientation. The events observed entail nonreciprocal exchange of genetic material, and involve a discrete nucleotide sequences that is duplicated in rotationally symmetrical reverse orientation on plasmid DNA (i.e., inverted repeat; palindrome)."}
{"id": "PMID:1093181", "title": "Phenylalanyl-tRNA synthetase from baker's yeast: role of 3'-terminal adenosine of tRNA-Phe in enzyme-substrate interaction studied with 3'-modified tRNA-Phe species.", "content": "TRNA-Phe species from baker's yeast modified at the 3'-terminus in many cases are phenylalanylatable substrates. Out of several tRNA-Phe species possessing a modified 3'-end that cannot be phenylalanylated, only two, tRNA-Phe-C-C-2'dA and the tRNA-Phe-C-C-formycin-oxi-red, are strong competitive inhibitors for tRNA-Phe-C-C-A during phenylalanylation. In the ATP/PPi exchange, both these inhibitors reduce Vmax to about 25%; but whereas tRNA-Phe-C-C-2dA has no influence on KmATP and Km Phe during ATP/PPi exchange, tRNA-Phe-C-C-formycin-oxi-red reduces KmATP from 1430 muM, found in the absence of tRNA-Phe, to 230 muM, and Km-Phe, from 38 to 14 muM. The values found in the presence of tRNA-Phe-C-C-formycin-oxi-red during ATP/PPi exchange are identical with those determined in the phenylalanylation of tRNA-Phe-C-C-A. All other tRNA-Phe species carrying a modified 3'end that cannot be phenylalanylated exhibit a mixed competitive-noncompetitive inhibition in the phenylalanylation reaction. In the ATP/PPi exchange, they do not influence KmATP and KmPHE  and only weakly, if at all, Vmax. The results show that the 3'adenosine of tRNA-Phe cannot solely be a passive acceptor for phenylalanine, but must in addition play an active role during enzyme-substrate interaction. The data can be consistently explained by the hypothesis that the 3'-adenosine of tRNA-Phe triggers a conformational change of the enzyme.", "contents": "Phenylalanyl-tRNA synthetase from baker's yeast: role of 3'-terminal adenosine of tRNA-Phe in enzyme-substrate interaction studied with 3'-modified tRNA-Phe species. TRNA-Phe species from baker's yeast modified at the 3'-terminus in many cases are phenylalanylatable substrates. Out of several tRNA-Phe species possessing a modified 3'-end that cannot be phenylalanylated, only two, tRNA-Phe-C-C-2'dA and the tRNA-Phe-C-C-formycin-oxi-red, are strong competitive inhibitors for tRNA-Phe-C-C-A during phenylalanylation. In the ATP/PPi exchange, both these inhibitors reduce Vmax to about 25%; but whereas tRNA-Phe-C-C-2dA has no influence on KmATP and Km Phe during ATP/PPi exchange, tRNA-Phe-C-C-formycin-oxi-red reduces KmATP from 1430 muM, found in the absence of tRNA-Phe, to 230 muM, and Km-Phe, from 38 to 14 muM. The values found in the presence of tRNA-Phe-C-C-formycin-oxi-red during ATP/PPi exchange are identical with those determined in the phenylalanylation of tRNA-Phe-C-C-A. All other tRNA-Phe species carrying a modified 3'end that cannot be phenylalanylated exhibit a mixed competitive-noncompetitive inhibition in the phenylalanylation reaction. In the ATP/PPi exchange, they do not influence KmATP and KmPHE  and only weakly, if at all, Vmax. The results show that the 3'adenosine of tRNA-Phe cannot solely be a passive acceptor for phenylalanine, but must in addition play an active role during enzyme-substrate interaction. The data can be consistently explained by the hypothesis that the 3'-adenosine of tRNA-Phe triggers a conformational change of the enzyme."}
{"id": "PMID:1093182", "title": "Three steps in conversion of large precursor RNA into serine and proline transfer RNAs.", "content": "Bacteriophage T4 serine and proline transfer RNAs are derived from a common precursor RNA. This precusor RNA lacks -C-C-A sequences which could provide 3' termini for the mature transfer RNAs. We have deduced part of the pathway leading to the formation of the C-C-A sequences in the transfer RNAs by characterizing incompletely matured precursor molecules which accumulate during infection of mutant hosts that lack specific enzymes associated with transfer RNA metabolism. Maturation is initiated by the addition of -C-C-AOH to the 3' terminus of the precusor RNA through the combined actionof an unidentified nuclease and tRNA nucleotidyltransferase (EC 2.7.7.25). Precursor RNA molecules terminating in -C-C-AOH is serine transfer RNA and the second product is immature proline transfer RNA. The terminal steps leading to proline transfer RNA have not been fully delineated, but are known to involve the replacement of a -C-UOH sequence by -C-C-AOH.", "contents": "Three steps in conversion of large precursor RNA into serine and proline transfer RNAs. Bacteriophage T4 serine and proline transfer RNAs are derived from a common precursor RNA. This precusor RNA lacks -C-C-A sequences which could provide 3' termini for the mature transfer RNAs. We have deduced part of the pathway leading to the formation of the C-C-A sequences in the transfer RNAs by characterizing incompletely matured precursor molecules which accumulate during infection of mutant hosts that lack specific enzymes associated with transfer RNA metabolism. Maturation is initiated by the addition of -C-C-AOH to the 3' terminus of the precusor RNA through the combined actionof an unidentified nuclease and tRNA nucleotidyltransferase (EC 2.7.7.25). Precursor RNA molecules terminating in -C-C-AOH is serine transfer RNA and the second product is immature proline transfer RNA. The terminal steps leading to proline transfer RNA have not been fully delineated, but are known to involve the replacement of a -C-UOH sequence by -C-C-AOH."}
{"id": "PMID:1093183", "title": "Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.", "content": "The trinucleotide AUG was condensed at the 5'-end with N-bromoacetyl-p-aminophenylphosphate. This bromoactylated AUG analog reacted irreversibly with the mRNA binding site of Escherichia coli 70S ribosomes. After reaction of 70S ribosomes with the AUG analog, labeled 30S subunits could be isolated that were programmed for initiation-factor-dependent binding of fMet-tRNAfMet. This shows that this AUG-affinity label reacted in the decoding site for fMet-tRNAfMet. By combination of sodium dodecyl sulfate-, Sarkosyl-, and ureapolyacrylamide gel electrophoresis the AUG-affinity label was found to be irreversibly bound to ribosomal proteins S4, the ram gene product, and S18.", "contents": "Affinity labeling of the ribosomal decoding site with an AUG-substrate analog. The trinucleotide AUG was condensed at the 5'-end with N-bromoacetyl-p-aminophenylphosphate. This bromoactylated AUG analog reacted irreversibly with the mRNA binding site of Escherichia coli 70S ribosomes. After reaction of 70S ribosomes with the AUG analog, labeled 30S subunits could be isolated that were programmed for initiation-factor-dependent binding of fMet-tRNAfMet. This shows that this AUG-affinity label reacted in the decoding site for fMet-tRNAfMet. By combination of sodium dodecyl sulfate-, Sarkosyl-, and ureapolyacrylamide gel electrophoresis the AUG-affinity label was found to be irreversibly bound to ribosomal proteins S4, the ram gene product, and S18."}
{"id": "PMID:1093184", "title": "A ribonucleoprotein precursor of both the 30S and 50S ribosomal sunbunits of Escherichia coli.", "content": "A ribonucleoprotein particle (46S) has been isolated from [3H]uridine pulse-labeled cultures of E. Coli AB301/105. Evidence from pulse chase experiments and from protein analysis suggested that this particle may give rise to both the 30S and 50S ribosomal subunits. Direct deproteinization of the particle yielded 30S RNA, while deproteinization after treatment with a crude RNase III preparation yielded products similar to 23S and 16S RNA. This result is consistent with the idea that the 46S ribonucleoprotein is the in vivo counterpart of 30S RNA, which is the in vitro product obtained after phenol extraction.", "contents": "A ribonucleoprotein precursor of both the 30S and 50S ribosomal sunbunits of Escherichia coli. A ribonucleoprotein particle (46S) has been isolated from [3H]uridine pulse-labeled cultures of E. Coli AB301/105. Evidence from pulse chase experiments and from protein analysis suggested that this particle may give rise to both the 30S and 50S ribosomal subunits. Direct deproteinization of the particle yielded 30S RNA, while deproteinization after treatment with a crude RNase III preparation yielded products similar to 23S and 16S RNA. This result is consistent with the idea that the 46S ribonucleoprotein is the in vivo counterpart of 30S RNA, which is the in vitro product obtained after phenol extraction."}
{"id": "PMID:1093185", "title": "Cell surface alterations induced by vaccinia and Newcastle disease viruses (38486).", "content": "A comparison was made of HEp-2 cell surface changes induced by NDV or vaccinia virus infection. Three parameters were examined as a function of time after infection: the kinetics of hemadosorption and the appearance of concanavalin (con A) binding sites, and alterations in electrophoretic mobility of single cells. The kinetics of appearance of con A binding sites was strikingly similar for both virus infections, whereas hemadsorption preceded NDA synthesis and followed vaccinia synthesis. These data suggest that in the vaccinia-infected cell the hemadsorption and con A binding sites are different. NDV infection or exposure of sham-infected cells to bacterial neuraminidase significantly reduced their anodal mobilities. This also occurred after enzyme treatment of vaccinia-infected cells. Measurements of the sialic acid content of NDV or sham-infected cells before and after neuraminidase treatment indicated the exposure to the enzyme or NDV materially reduced the sialic acid content of cells. Vaccinia-infected cells contained considerably more sialic acid than did normal cells. For  the vaccinia-infected cell a change in surface properties as detected by hemadsorption or increased con A binding was not reflected in a change in electrophoretic mobility.", "contents": "Cell surface alterations induced by vaccinia and Newcastle disease viruses (38486). A comparison was made of HEp-2 cell surface changes induced by NDV or vaccinia virus infection. Three parameters were examined as a function of time after infection: the kinetics of hemadosorption and the appearance of concanavalin (con A) binding sites, and alterations in electrophoretic mobility of single cells. The kinetics of appearance of con A binding sites was strikingly similar for both virus infections, whereas hemadsorption preceded NDA synthesis and followed vaccinia synthesis. These data suggest that in the vaccinia-infected cell the hemadsorption and con A binding sites are different. NDV infection or exposure of sham-infected cells to bacterial neuraminidase significantly reduced their anodal mobilities. This also occurred after enzyme treatment of vaccinia-infected cells. Measurements of the sialic acid content of NDV or sham-infected cells before and after neuraminidase treatment indicated the exposure to the enzyme or NDV materially reduced the sialic acid content of cells. Vaccinia-infected cells contained considerably more sialic acid than did normal cells. For  the vaccinia-infected cell a change in surface properties as detected by hemadsorption or increased con A binding was not reflected in a change in electrophoretic mobility."}
{"id": "PMID:1093186", "title": "Ultrastructral effects of in virto experimentation on right ventricular papillary muscle from cats in hypovolemic shock (38487).", "content": "The present data demonstrate for the first time that repeated stimulation (2-3 hr in an oxygenated perfusate) of right ventricular papillary muscles from cats subjected to hypovolemic shock does not alter the ultrastructure of the shock lesions. Neither does the in vitro experimentation cause functional impairment as determined by the measured isometric twitch tension. The data also indicate that electron microscopy is important to the evaluation of papillary muscles from cats subjected to shock.", "contents": "Ultrastructral effects of in virto experimentation on right ventricular papillary muscle from cats in hypovolemic shock (38487). The present data demonstrate for the first time that repeated stimulation (2-3 hr in an oxygenated perfusate) of right ventricular papillary muscles from cats subjected to hypovolemic shock does not alter the ultrastructure of the shock lesions. Neither does the in vitro experimentation cause functional impairment as determined by the measured isometric twitch tension. The data also indicate that electron microscopy is important to the evaluation of papillary muscles from cats subjected to shock."}
{"id": "PMID:1093187", "title": "A mode of the growth of Mycobacterium lepraemurium in a cell-free liquid medium (38501).", "content": "The first report regarding in vitro growth patterns of M. lepraemurium was presented. Evidence is indicated that a growth mode of a single cell of M.lepraemurium in vitro is similar to that of culturable mycobacterium, especially atypical mycobacterium. This finding has added one property to characteristics of M. lepraemurium which have been already known.", "contents": "A mode of the growth of Mycobacterium lepraemurium in a cell-free liquid medium (38501). The first report regarding in vitro growth patterns of M. lepraemurium was presented. Evidence is indicated that a growth mode of a single cell of M.lepraemurium in vitro is similar to that of culturable mycobacterium, especially atypical mycobacterium. This finding has added one property to characteristics of M. lepraemurium which have been already known."}
{"id": "PMID:1093188", "title": "Development of response to glucose of fetal rat islet in organ culture (38479).", "content": "Pancreases of 18-day fetal and 3-day neonatal rat were grown in organ culture in both standard glucose (150 mg/100 ml) and high glucose (500 mg/100 ml) media. Insulin content of medium was measured by radioimmunoassay at time of transfer. In fetal cultures, standard and high glucose media contained a similar level of insulin through 6 days of culture. In neonatal cultures, high glucose medium contained 40-60% more insulin than did standard medium. However, after 8 days of organ culture fetal pancreas developed responsiveness to high glucose; a greater amount of insulin was present in the high glucose medium (50% by 10 days and 70% by 12 days) than in standard glucose medium. The time at which this responsiveness develops in vitro approximates the chronologic age which corresponds to 3-5 days postnatal period. The maturation of this responsiveness appears to be inherent to the pancreas and is independent of other organ systems. When neonatal explants, grown in standard medium for 8 days, were transferred to high glucose medium, 80-160% more insulin was detected in the high glucose medium than in standard medium during the next 4 days of culture. These results indicate that once glucose responsiveness had developed, it is maintained in organ culture for at least 12 days.", "contents": "Development of response to glucose of fetal rat islet in organ culture (38479). Pancreases of 18-day fetal and 3-day neonatal rat were grown in organ culture in both standard glucose (150 mg/100 ml) and high glucose (500 mg/100 ml) media. Insulin content of medium was measured by radioimmunoassay at time of transfer. In fetal cultures, standard and high glucose media contained a similar level of insulin through 6 days of culture. In neonatal cultures, high glucose medium contained 40-60% more insulin than did standard medium. However, after 8 days of organ culture fetal pancreas developed responsiveness to high glucose; a greater amount of insulin was present in the high glucose medium (50% by 10 days and 70% by 12 days) than in standard glucose medium. The time at which this responsiveness develops in vitro approximates the chronologic age which corresponds to 3-5 days postnatal period. The maturation of this responsiveness appears to be inherent to the pancreas and is independent of other organ systems. When neonatal explants, grown in standard medium for 8 days, were transferred to high glucose medium, 80-160% more insulin was detected in the high glucose medium than in standard medium during the next 4 days of culture. These results indicate that once glucose responsiveness had developed, it is maintained in organ culture for at least 12 days."}
{"id": "PMID:1093189", "title": "The effect of magnesium deficiency in mice on serum immunoglobulin concentrations and antibody plaque-forming cells.", "content": "Magnesium-deficient mice immunized with SRBC showed a significant decrease (P less than 0.001) in the number of PFC in their spleens compared with mice on a control diet. Serum immunoglobulin concentrations (IgG1, IgG2, IgA and IgM) were determined after 3, 6, 9 and 12 days on the respective diets. The serum IgG2 and IgM concentrations of magnesium- deficient mice were decreased (P less than 0.005) by 6 days and remained at these concentrations until 12 days. The serum IgG1 and IgA concentrations of magnesium deficient animals also decreased (P less than 0.01) by 6 days but returned toward control concentrations at 12 days. Serum magnesium concentrations confirmed the magnesium deficiency of the experimental animals. Therefore, magnesium deficiency has profound immunosuppressive capabilities in mice by significantly reducing the number of antibody synthesizing cells and serum immunoglobulin concentrations.", "contents": "The effect of magnesium deficiency in mice on serum immunoglobulin concentrations and antibody plaque-forming cells. Magnesium-deficient mice immunized with SRBC showed a significant decrease (P less than 0.001) in the number of PFC in their spleens compared with mice on a control diet. Serum immunoglobulin concentrations (IgG1, IgG2, IgA and IgM) were determined after 3, 6, 9 and 12 days on the respective diets. The serum IgG2 and IgM concentrations of magnesium- deficient mice were decreased (P less than 0.005) by 6 days and remained at these concentrations until 12 days. The serum IgG1 and IgA concentrations of magnesium deficient animals also decreased (P less than 0.01) by 6 days but returned toward control concentrations at 12 days. Serum magnesium concentrations confirmed the magnesium deficiency of the experimental animals. Therefore, magnesium deficiency has profound immunosuppressive capabilities in mice by significantly reducing the number of antibody synthesizing cells and serum immunoglobulin concentrations."}
{"id": "PMID:1093190", "title": "Effect of synthetic luteinizing hormone--releasing hormone in newborn rats.", "content": "Administration of synthetic LH-RH to male and female rats on the first day of life reduced pituitary content of FSH and LH and increased serum concentrations of LH.", "contents": "Effect of synthetic luteinizing hormone--releasing hormone in newborn rats. Administration of synthetic LH-RH to male and female rats on the first day of life reduced pituitary content of FSH and LH and increased serum concentrations of LH."}
{"id": "PMID:1093191", "title": "Mechanisms of endotoxin tolerance. IX. Effect of exchange transfusion.", "content": "Healthy New Zealand rabbits were injected iv with an LD-80 dose of E. coli endotoxin.  Twenty minutes later, after removal of over 50% of the endotoxin by the RES, exchange transfusion was performed, accomplishing a rapid and sustained reduction in the level of endotoxemia simulating that seen in animals rendered highly tolerant by seven prior sublethal injections of toxin.  Depite such reduction in endotoxemia, 96-hr mortality was only slightly, and not significantly reduced compared to sham exchanged controls (70 vs 83% respectively).  Additional control studies indicated that exchange tranfusion per se did not enhance endotoxin mortality.  The findings directly support the concept that endotoxin tolerance is based primarily upon enhanced RES resistance to endotoxin toxicity rather than upon enhanced RES clearance of circulating endotoxin.", "contents": "Mechanisms of endotoxin tolerance. IX. Effect of exchange transfusion. Healthy New Zealand rabbits were injected iv with an LD-80 dose of E. coli endotoxin.  Twenty minutes later, after removal of over 50% of the endotoxin by the RES, exchange transfusion was performed, accomplishing a rapid and sustained reduction in the level of endotoxemia simulating that seen in animals rendered highly tolerant by seven prior sublethal injections of toxin.  Depite such reduction in endotoxemia, 96-hr mortality was only slightly, and not significantly reduced compared to sham exchanged controls (70 vs 83% respectively).  Additional control studies indicated that exchange tranfusion per se did not enhance endotoxin mortality.  The findings directly support the concept that endotoxin tolerance is based primarily upon enhanced RES resistance to endotoxin toxicity rather than upon enhanced RES clearance of circulating endotoxin."}
{"id": "PMID:1093192", "title": "Immunosuppressive effects of experimental infection with Plasmodium gallinaceum.", "content": "Experimental infection of chickens with P. gallinaceum mardedly suppressed the splenic PFC response to SRBC.  Suppression was most pronounced when birds were immunized at the time of peak parasitemia.  The PFC response to E. coli LPS was of low magnitiude in both normal and infected chickens; however, it, too, was suppressed in infected birds, but not to the same degree as observed in response to SRBC.  Cellular immunity as evidenced by allograft rejection was not influenced by infection.", "contents": "Immunosuppressive effects of experimental infection with Plasmodium gallinaceum. Experimental infection of chickens with P. gallinaceum mardedly suppressed the splenic PFC response to SRBC.  Suppression was most pronounced when birds were immunized at the time of peak parasitemia.  The PFC response to E. coli LPS was of low magnitiude in both normal and infected chickens; however, it, too, was suppressed in infected birds, but not to the same degree as observed in response to SRBC.  Cellular immunity as evidenced by allograft rejection was not influenced by infection."}
{"id": "PMID:1093193", "title": "Failure of peritoneal exudate macrophages to reverse immunologic impairment by Friend leukemia virus.", "content": "Transfer experiments with peritoneal exudate macrophages from normal donor mice were performed to determine if a defect of normal macrophage function or activity was a major or contributing factor to the immunosuppression characterizing leukemia virus infection of mice. Challenge immunization of Friend leukemia virus-infected mice with sheep erythrocytes resulted in markedly depressed hemolytic antibody responses, as compared to responses of normal noninfected mice. When PE cell suspensions rich in macrophages were transferred from normal donor mice to leukemia virus infected recipients there was no affect on the FLV-induced impairment of the immune response. Similar transfer of PE cells to normal uninfected mice generally resulted in a moderate depression of the expected immune response. In no case did the PE cells enhance the immune responses in normal or virus-infected mice.", "contents": "Failure of peritoneal exudate macrophages to reverse immunologic impairment by Friend leukemia virus. Transfer experiments with peritoneal exudate macrophages from normal donor mice were performed to determine if a defect of normal macrophage function or activity was a major or contributing factor to the immunosuppression characterizing leukemia virus infection of mice. Challenge immunization of Friend leukemia virus-infected mice with sheep erythrocytes resulted in markedly depressed hemolytic antibody responses, as compared to responses of normal noninfected mice. When PE cell suspensions rich in macrophages were transferred from normal donor mice to leukemia virus infected recipients there was no affect on the FLV-induced impairment of the immune response. Similar transfer of PE cells to normal uninfected mice generally resulted in a moderate depression of the expected immune response. In no case did the PE cells enhance the immune responses in normal or virus-infected mice."}
{"id": "PMID:1093194", "title": "Inhibition of LH release as influenced by LRF infusion.", "content": "Four ovariectomized ewes were infused with LRF (10 mug/hr for 20 hr.  Jugular vein blood samples were taken at 30 min intervals.  After 16 hr of LRF infusion each animal was given a 1 mg iv injection of LRF.  At the end of the 20 hr infusion period, the animals were sacrificed and the pituitary removed.  Pituitary and plasma samples were analyzed for LH.  Plasma LH concentration reached a peak level of 73 ng/ml, 1.5 hr after starting the LRF infusion.  A rapid decline in LH concentration occured for the next 3 hr.  The plasma LH concentration declined slowly to preinfusion levels over the next 15.5 hr.  A small insigificant rise in plasma LH concentration occured when the animals were given 1 mg of LRF after 16 hr of LRF infusion.  At the end of the treatment period pituitary LH concentration varied from 369 to 1360 ng/mg.  Two of the four ewes had pituitary LH concentrations similar to untreated ovariectomized ewes.  The pituitarys' refractoriness to LRF apparently is not due to depletion of pituitary LH.", "contents": "Inhibition of LH release as influenced by LRF infusion. Four ovariectomized ewes were infused with LRF (10 mug/hr for 20 hr.  Jugular vein blood samples were taken at 30 min intervals.  After 16 hr of LRF infusion each animal was given a 1 mg iv injection of LRF.  At the end of the 20 hr infusion period, the animals were sacrificed and the pituitary removed.  Pituitary and plasma samples were analyzed for LH.  Plasma LH concentration reached a peak level of 73 ng/ml, 1.5 hr after starting the LRF infusion.  A rapid decline in LH concentration occured for the next 3 hr.  The plasma LH concentration declined slowly to preinfusion levels over the next 15.5 hr.  A small insigificant rise in plasma LH concentration occured when the animals were given 1 mg of LRF after 16 hr of LRF infusion.  At the end of the treatment period pituitary LH concentration varied from 369 to 1360 ng/mg.  Two of the four ewes had pituitary LH concentrations similar to untreated ovariectomized ewes.  The pituitarys' refractoriness to LRF apparently is not due to depletion of pituitary LH."}
{"id": "PMID:1093195", "title": "Effect of intermittent infusion of LH-releasing hormone of serum LH and FSH levels in immature male rats.", "content": "The effect of intermittent intravenous infusions of LH-RH on serum LH and FSH levels was studied in immature male rats. At the end of the infusion, serum LH and FSH levels were higher than those after saline infusion. One hr after the end of infusion of LH-RH, serum LH levels fell, reaching saline control values, but FSH levels remained higher. Moreover, a pulsative pattern of LH release but not of FSH, was obtained during the intermittent infusion with LH-RH. These findings indicate that under appropriate conditions after administration of LH-RH, it is possible to find high GSH levels without a concomitant rise of LH levels. The continued elevation of FSH may be explained by the longer biological half-life of FSH. Estradiol benzoate depressed the basal serum GSH level and augmented the basal serum GSH level and augmented LH release after intermittent infusion with LH-RH, indicating that estrogen modified FSH/LH ratio. The results suggest that discordance of plasma LH and FSH levels which is occasionally observed in some physiological conditions does not necessarily indicate the presence of another FSH-RH which is distinct from LH-RH decapeptide.", "contents": "Effect of intermittent infusion of LH-releasing hormone of serum LH and FSH levels in immature male rats. The effect of intermittent intravenous infusions of LH-RH on serum LH and FSH levels was studied in immature male rats. At the end of the infusion, serum LH and FSH levels were higher than those after saline infusion. One hr after the end of infusion of LH-RH, serum LH levels fell, reaching saline control values, but FSH levels remained higher. Moreover, a pulsative pattern of LH release but not of FSH, was obtained during the intermittent infusion with LH-RH. These findings indicate that under appropriate conditions after administration of LH-RH, it is possible to find high GSH levels without a concomitant rise of LH levels. The continued elevation of FSH may be explained by the longer biological half-life of FSH. Estradiol benzoate depressed the basal serum GSH level and augmented the basal serum GSH level and augmented LH release after intermittent infusion with LH-RH, indicating that estrogen modified FSH/LH ratio. The results suggest that discordance of plasma LH and FSH levels which is occasionally observed in some physiological conditions does not necessarily indicate the presence of another FSH-RH which is distinct from LH-RH decapeptide."}
{"id": "PMID:1093196", "title": "Alteration of bone marrow-thymus cell synergism in hereditary asplenic and adult splenctomiced mice.", "content": "The cooperation betweeen bone marrow (B) and thymus (T) cells was markedly impaired in mice with congenital asplenia.  The deficiency of IgM producers could not be corrected by neotal transplantation of spleen cells.  The use of T and B cells from splenectomized donors resulted in a marked shift from 19S to 7S antibody forming cells.", "contents": "Alteration of bone marrow-thymus cell synergism in hereditary asplenic and adult splenctomiced mice. The cooperation betweeen bone marrow (B) and thymus (T) cells was markedly impaired in mice with congenital asplenia.  The deficiency of IgM producers could not be corrected by neotal transplantation of spleen cells.  The use of T and B cells from splenectomized donors resulted in a marked shift from 19S to 7S antibody forming cells."}
{"id": "PMID:1093197", "title": "The effect of oral and vaginal administration of synthetic LH-RH and [D-ALA-6, DES GLY-10-NH2]-LH-RH ethylamide on serum LH levels in ovariectomized, steroid blocked rats.", "content": "Effects of oral and vaginal administration of LH-RH and [D-ALa-6, DesGly-10-NH2i1-LH-RH ethylamide (D-Ala-6-LH-RH-EA) on serum LH levels in ovariectomized, estrogen, progesterone treated rats were investigated. Oral administration of synthetic LH-RH induced a quick rise of serum LH levels with the greatest elevation at 15 min at any dose levels tested. On the other hand, oral administration of D-Ala-6-LH-RH-EA resulted in a slow but progressive rise of LH during 120 min of observation. The total amount of LH released by 10 mug of the analog was much greater than the total released by 1000 mug of LH-RH. Vaginal administration of 100 mug of LH-RH mixed with Carbowax induced a progressive rise of LHwhich was indistinguishable from that following 10 mug of the analogue, suggesting that the potency of the analogue is 10 times greater than that of LH-RH for vaginal administration. Ten mug of LH-RH given through the vagina induced a rapid rise of LH with the peak at 15 min, whereas 1 mug of the analog induced a slow but progressive rise. Greater resistance of D-Ala-6-LH-RH-EA than LH-RH against in vivo breakdown is postulated as one of the causes of greater and prolonged LH release by the former.", "contents": "The effect of oral and vaginal administration of synthetic LH-RH and [D-ALA-6, DES GLY-10-NH2]-LH-RH ethylamide on serum LH levels in ovariectomized, steroid blocked rats. Effects of oral and vaginal administration of LH-RH and [D-ALa-6, DesGly-10-NH2i1-LH-RH ethylamide (D-Ala-6-LH-RH-EA) on serum LH levels in ovariectomized, estrogen, progesterone treated rats were investigated. Oral administration of synthetic LH-RH induced a quick rise of serum LH levels with the greatest elevation at 15 min at any dose levels tested. On the other hand, oral administration of D-Ala-6-LH-RH-EA resulted in a slow but progressive rise of LH during 120 min of observation. The total amount of LH released by 10 mug of the analog was much greater than the total released by 1000 mug of LH-RH. Vaginal administration of 100 mug of LH-RH mixed with Carbowax induced a progressive rise of LHwhich was indistinguishable from that following 10 mug of the analogue, suggesting that the potency of the analogue is 10 times greater than that of LH-RH for vaginal administration. Ten mug of LH-RH given through the vagina induced a rapid rise of LH with the peak at 15 min, whereas 1 mug of the analog induced a slow but progressive rise. Greater resistance of D-Ala-6-LH-RH-EA than LH-RH against in vivo breakdown is postulated as one of the causes of greater and prolonged LH release by the former."}
{"id": "PMID:1093198", "title": "Electrocardiographic recordings for assessing survival of cardiac allografts in the hamster cheek pouch.", "content": "Techniques for the recording of electrical events associated with contraction of allografted myocardium in the cheek pouch of inbred hamsters have been developed. The results of such recordings have been useful in assessing contractility and viability of the graft. The degree of tolerance to cardiac allografts in cheek pouches of inbred hamsters appears to be a function of histocompatibility and not \"immunological privilege\" of the cheek pouch.", "contents": "Electrocardiographic recordings for assessing survival of cardiac allografts in the hamster cheek pouch. Techniques for the recording of electrical events associated with contraction of allografted myocardium in the cheek pouch of inbred hamsters have been developed. The results of such recordings have been useful in assessing contractility and viability of the graft. The degree of tolerance to cardiac allografts in cheek pouches of inbred hamsters appears to be a function of histocompatibility and not \"immunological privilege\" of the cheek pouch."}
{"id": "PMID:1093199", "title": "A23187: a calcium ionophore that directly increases cardiac contractility (38704).", "content": "The effect of the calcium ionophore A23187 on contractility in guinea pig left atria was studied. This ionophore increased both the force of contraction and the rate of tension development in a concentration dependent manner. The positive inotropic effect of A23187 is not mediated by activation of beta-receptors or by the release of endogenous catecholamines since neither propranolol nor pretreatment with reserpine altered the inotropic effect of this agent. Our studies of the dependence of contractile force on external calcium indicate that A23187 enhances the response of atrial muscle to external calcium ions. Since A23187 is known to facilitate the movement of calcium across biological membranes, we believe the ionophore may be acting either by increasing calcium influx across the myocardial cell membrane or by facilitating calcium release from sarcoplasmic reticulum.", "contents": "A23187: a calcium ionophore that directly increases cardiac contractility (38704). The effect of the calcium ionophore A23187 on contractility in guinea pig left atria was studied. This ionophore increased both the force of contraction and the rate of tension development in a concentration dependent manner. The positive inotropic effect of A23187 is not mediated by activation of beta-receptors or by the release of endogenous catecholamines since neither propranolol nor pretreatment with reserpine altered the inotropic effect of this agent. Our studies of the dependence of contractile force on external calcium indicate that A23187 enhances the response of atrial muscle to external calcium ions. Since A23187 is known to facilitate the movement of calcium across biological membranes, we believe the ionophore may be acting either by increasing calcium influx across the myocardial cell membrane or by facilitating calcium release from sarcoplasmic reticulum."}
{"id": "PMID:1093200", "title": "Method for differentiation of nonspecific from specfic toxoplasma IgM fluorescent antibodies in patients with rheumatoid factor.", "content": "In a study performed to define the prevalence of false positive toxoplasma IgM-IFA test results in sera containing RF, 8 (19.5%) of 41 sera which were positive for RF were positive in the toxoplasma DT and conventional toxoplasma IFA test. Three of these eight were also positive in the toxoplasma IgM-IFA test and in two, the results were considered to be false positives. Of the 33 sera remaining which were positive for RF but negative in both the DT and conventional IFA test, three were positive in the toxoplasma IgM-IFA test. Of 51 sera from patients with suspected rheumatoid arthritis or other collagen vascular disorders, all of which were negative when tested for RF, none was positive for toxoplasma IgM antibodies in the IgM-IFA test. Sera from 15 adults with the acute lymphadenopathic form of toxoplasmosis and 13 infants with congenital toxoplasmosis were tested for the presence of RF. Whereas none of the sera from the acquired cases had demonstrable RF, two of the congenital cases had RF, and their titers were both 1:320. False positive IgM-IFA test results became negative after treatment of sera with heat-aggregated IgG. In contrast, IgM-IFA test titers in cases of acute congenital or acquired toxoplasmosis were unaffected by this treatment. Thus, treatment with heat-aggregated IgG can be used to differentiate false positive IgM-IFA test titers due to RF from those due to specific IgM toxoplasma antibody.", "contents": "Method for differentiation of nonspecific from specfic toxoplasma IgM fluorescent antibodies in patients with rheumatoid factor. In a study performed to define the prevalence of false positive toxoplasma IgM-IFA test results in sera containing RF, 8 (19.5%) of 41 sera which were positive for RF were positive in the toxoplasma DT and conventional toxoplasma IFA test. Three of these eight were also positive in the toxoplasma IgM-IFA test and in two, the results were considered to be false positives. Of the 33 sera remaining which were positive for RF but negative in both the DT and conventional IFA test, three were positive in the toxoplasma IgM-IFA test. Of 51 sera from patients with suspected rheumatoid arthritis or other collagen vascular disorders, all of which were negative when tested for RF, none was positive for toxoplasma IgM antibodies in the IgM-IFA test. Sera from 15 adults with the acute lymphadenopathic form of toxoplasmosis and 13 infants with congenital toxoplasmosis were tested for the presence of RF. Whereas none of the sera from the acquired cases had demonstrable RF, two of the congenital cases had RF, and their titers were both 1:320. False positive IgM-IFA test results became negative after treatment of sera with heat-aggregated IgG. In contrast, IgM-IFA test titers in cases of acute congenital or acquired toxoplasmosis were unaffected by this treatment. Thus, treatment with heat-aggregated IgG can be used to differentiate false positive IgM-IFA test titers due to RF from those due to specific IgM toxoplasma antibody."}
{"id": "PMID:1093201", "title": "Ovulation and gonadotropin-releasing activity of [p-LEU-6, DES-GLY NH2-10, pro-ethylamide-9] -GNRH.", "content": "The ovulation-inducing and gonadotropin-releasing activities of [d-Leu-6, des-Gly NH2-10,  Pro-ethylamide-9]-GnRH (II), were evaluated in rats, rabbits, and sheep. A sc dose of 3.4 ng/100 g body wt of the analog was equal to 160 ng/100 g body wt of GnRH in causing ovulation in the diestrous rat. At these dose levels, the integrated LH release was 1.9 times greater for the analog. Both the time of increase and maximum serum concentrations of LH were delayed after injection of the analog. Oral administration of II and GnRH to the proestrous rat resulted in an ED50 for ovulation of 0.92 and 54 mug/100 g body wt,respectively. Serum levels of LH and FSH were highly variable for the various treatment groups when both releasing substances were administered orally. The intense ovulating activity of II was also evident in the estrous rabbit as indicated by an activity 31 times greater than that of GnRH. Additionally, the analog was at least 50 times more active than GnRH in releasing LH in both the mid-luteal and anestrous ewe. From our experiments with the cycling rat it appears that the intense ovulation-inducing activity of II can be accounted for by the intrinsic LH-releasing activity of the nonapeptide, rather than by a prolonged release stimulus.", "contents": "Ovulation and gonadotropin-releasing activity of [p-LEU-6, DES-GLY NH2-10, pro-ethylamide-9] -GNRH. The ovulation-inducing and gonadotropin-releasing activities of [d-Leu-6, des-Gly NH2-10,  Pro-ethylamide-9]-GnRH (II), were evaluated in rats, rabbits, and sheep. A sc dose of 3.4 ng/100 g body wt of the analog was equal to 160 ng/100 g body wt of GnRH in causing ovulation in the diestrous rat. At these dose levels, the integrated LH release was 1.9 times greater for the analog. Both the time of increase and maximum serum concentrations of LH were delayed after injection of the analog. Oral administration of II and GnRH to the proestrous rat resulted in an ED50 for ovulation of 0.92 and 54 mug/100 g body wt,respectively. Serum levels of LH and FSH were highly variable for the various treatment groups when both releasing substances were administered orally. The intense ovulating activity of II was also evident in the estrous rabbit as indicated by an activity 31 times greater than that of GnRH. Additionally, the analog was at least 50 times more active than GnRH in releasing LH in both the mid-luteal and anestrous ewe. From our experiments with the cycling rat it appears that the intense ovulation-inducing activity of II can be accounted for by the intrinsic LH-releasing activity of the nonapeptide, rather than by a prolonged release stimulus."}
{"id": "PMID:1093202", "title": "Immunoglobulin levels and antibody-coated bacteria in urines from patients with urinary tract infections.", "content": "The presence of antibody-coated bacteria in urines from patients with urinary tract infections has previously been reported to correlate with renal infection as opposed to bladder infection. Urine specimens from 12 patients with pyelonephritis and 12 patients with cystitis were studied to determine whether the antibody coating the bacteria is associated with elevated urine levels of total protein or of particular classes of immunoglobulins. The classes of antibody bound to the infecting bacteria in urines from the patients with pyelonephritis were compared to the levels of unbound antibody in the urine. Each specimen was found to contain antibody-coated bacteria, but not all of the specimens had elevated levels of total protein or immunoglobulins. Thus, the occurrence of antibody-coated bacteria in pyelonephritis did not depend on marked elevations of total urinary protein or immunoglobulins. Studies of patients with cystitis showed that immunoglobulins and protein present in the urines, even in elevated quantities, did not react with the infecting bacteria in patients with bladder infections, as each of these patients had negative FA tests for antibody-coated bacteria.", "contents": "Immunoglobulin levels and antibody-coated bacteria in urines from patients with urinary tract infections. The presence of antibody-coated bacteria in urines from patients with urinary tract infections has previously been reported to correlate with renal infection as opposed to bladder infection. Urine specimens from 12 patients with pyelonephritis and 12 patients with cystitis were studied to determine whether the antibody coating the bacteria is associated with elevated urine levels of total protein or of particular classes of immunoglobulins. The classes of antibody bound to the infecting bacteria in urines from the patients with pyelonephritis were compared to the levels of unbound antibody in the urine. Each specimen was found to contain antibody-coated bacteria, but not all of the specimens had elevated levels of total protein or immunoglobulins. Thus, the occurrence of antibody-coated bacteria in pyelonephritis did not depend on marked elevations of total urinary protein or immunoglobulins. Studies of patients with cystitis showed that immunoglobulins and protein present in the urines, even in elevated quantities, did not react with the infecting bacteria in patients with bladder infections, as each of these patients had negative FA tests for antibody-coated bacteria."}
{"id": "PMID:1093203", "title": "Survival of osteocompetent marrow cells in vitro and the effect of PHA-stimulation on osteoinduction in composite bone grafts.", "content": "The osteoinductive capacities of bone-marrow autografts, washed marrow-free bone allografts, and composite grafts of washed allogeneic bone impregnated with viable or killed autologous marrow cells have been compared in a heterotopic site in rabbits. Composite grafts (similar to 40 mg allogeneic bone) with viable marrow performed equally well as autografts, providing that these implants contained at least 50,000 marrow cells. Composite grafts with dead marrow were noninductive, and grafts with 10,000 viable marrow cells produced much less new bone than grafts with 50,000 cells. Postoperative treatment of host rabbits with PHA-P increased the osteoinductive capacity of composite grafts with initially subcritical numbers of marrow cells to control levels. These results indicate that the osteoinductive cells in composite grafts are mobilized from the marrow elements rather than from the undifferentiated cells in the host bed.", "contents": "Survival of osteocompetent marrow cells in vitro and the effect of PHA-stimulation on osteoinduction in composite bone grafts. The osteoinductive capacities of bone-marrow autografts, washed marrow-free bone allografts, and composite grafts of washed allogeneic bone impregnated with viable or killed autologous marrow cells have been compared in a heterotopic site in rabbits. Composite grafts (similar to 40 mg allogeneic bone) with viable marrow performed equally well as autografts, providing that these implants contained at least 50,000 marrow cells. Composite grafts with dead marrow were noninductive, and grafts with 10,000 viable marrow cells produced much less new bone than grafts with 50,000 cells. Postoperative treatment of host rabbits with PHA-P increased the osteoinductive capacity of composite grafts with initially subcritical numbers of marrow cells to control levels. These results indicate that the osteoinductive cells in composite grafts are mobilized from the marrow elements rather than from the undifferentiated cells in the host bed."}
{"id": "PMID:1093211", "title": "Plasma scalpel excision of burns. An experimental study.", "content": "We used a plasma scalpel to excise experimental full-thickness burns in 12 pigs, followed by immediate skin autografting. The results were as follows: (1) The skin autograft takes were excellent following the plasma scalpel excisions, and they healed on essentially the same time scale as similar autografts following steel scalpel excisions, producing scars of similar sizes. (2) Pigs in which burn wounds were excised with a steel scalpel lost an average of approximately 5 gm of blood for every one gm lost in the animals which had excisions with the plasma scalpel. (3) The time required for a plasma scalpel burn excision was essentially the same as that required for a standard steel scalpel.", "contents": "Plasma scalpel excision of burns. An experimental study. We used a plasma scalpel to excise experimental full-thickness burns in 12 pigs, followed by immediate skin autografting. The results were as follows: (1) The skin autograft takes were excellent following the plasma scalpel excisions, and they healed on essentially the same time scale as similar autografts following steel scalpel excisions, producing scars of similar sizes. (2) Pigs in which burn wounds were excised with a steel scalpel lost an average of approximately 5 gm of blood for every one gm lost in the animals which had excisions with the plasma scalpel. (3) The time required for a plasma scalpel burn excision was essentially the same as that required for a standard steel scalpel."}
{"id": "PMID:1093212", "title": "Basal cell carcinoma of the medial canthal area.", "content": "We advise an aggressive approach in ablating basal cell carcinomas which have originated in the region of the medial canthus. Extirpation of the entire tumor under accurate histological control, whether it includes skin only or the entire orbital contents and ethmoid air cells--and reconstruction with one of the described procedures--gives the surgeon an effective armamentarium to successfully treat these potentially lethal lesions.", "contents": "Basal cell carcinoma of the medial canthal area. We advise an aggressive approach in ablating basal cell carcinomas which have originated in the region of the medial canthus. Extirpation of the entire tumor under accurate histological control, whether it includes skin only or the entire orbital contents and ethmoid air cells--and reconstruction with one of the described procedures--gives the surgeon an effective armamentarium to successfully treat these potentially lethal lesions."}
{"id": "PMID:1093213", "title": "A simplified technique for free transfer of groin flaps, by use of a Doppler Probe.", "content": "Our application of the Doppler probe to microvascular surgery is discussed, including the monitoring of the superficial circumflex iliac artery in free groin flaps. Our technique of free groin flap transplantation is compared to that of others. Three of our cases are discussed.", "contents": "A simplified technique for free transfer of groin flaps, by use of a Doppler Probe. Our application of the Doppler probe to microvascular surgery is discussed, including the monitoring of the superficial circumflex iliac artery in free groin flaps. Our technique of free groin flap transplantation is compared to that of others. Three of our cases are discussed."}
{"id": "PMID:1093214", "title": "Four V-flap repair of preputial stenosis (phimosis).", "content": "The 4 V-flap technique for repair of preputial stenosis meets a need for an alternative to circumcision in the repair of phimosis. It is especially useful in cases where the penis is small and all available tissue should be retained. we have used the method for 3 years and have had no complications. On occasion, we have used 6 or 8 V-flaps, but we find the 4 V-flap repair is usually simpler and equally effective.", "contents": "Four V-flap repair of preputial stenosis (phimosis). The 4 V-flap technique for repair of preputial stenosis meets a need for an alternative to circumcision in the repair of phimosis. It is especially useful in cases where the penis is small and all available tissue should be retained. we have used the method for 3 years and have had no complications. On occasion, we have used 6 or 8 V-flaps, but we find the 4 V-flap repair is usually simpler and equally effective."}
{"id": "PMID:1093215", "title": "Arteriovenous malformations of the mandible. Graduated surgical management.", "content": "Two cases of arteriovenous malformation in the mandible are described, in which muscle embolization was used. The success in one patient and the failure in the second correlate with the caliber of the vessels in the two malformations. A scheme of graduated surgical management for mandibular arteriovenous malformations is proposed.", "contents": "Arteriovenous malformations of the mandible. Graduated surgical management. Two cases of arteriovenous malformation in the mandible are described, in which muscle embolization was used. The success in one patient and the failure in the second correlate with the caliber of the vessels in the two malformations. A scheme of graduated surgical management for mandibular arteriovenous malformations is proposed."}
{"id": "PMID:1093218", "title": "The deltoscapular flap.", "content": "We present a young patient in whom a large soft tissue defect of the neck was repaired with a deltoscapular flap.", "contents": "The deltoscapular flap. We present a young patient in whom a large soft tissue defect of the neck was repaired with a deltoscapular flap."}
{"id": "PMID:1093226", "title": "Anterior extrapleural line: superior extension.", "content": "Anatomic sections of the superior aspect of the anterior mediastinum reveal that normal structures can cause indentations upon the adjacent lung. On lateral chest films, the soft-tissue density of the anterior superior mediastinal structures contrasts with that of the normal lung, revealing a normal, undulating configuration of the anterior superior mediastinum. We have termed this the \"vascular incisura,\" analogous to the cardiac incisura of the left lung seen inferiorly. An appreciation of normal variations within this space is essential when evaluating pathologic alterations.", "contents": "Anterior extrapleural line: superior extension. Anatomic sections of the superior aspect of the anterior mediastinum reveal that normal structures can cause indentations upon the adjacent lung. On lateral chest films, the soft-tissue density of the anterior superior mediastinal structures contrasts with that of the normal lung, revealing a normal, undulating configuration of the anterior superior mediastinum. We have termed this the \"vascular incisura,\" analogous to the cardiac incisura of the left lung seen inferiorly. An appreciation of normal variations within this space is essential when evaluating pathologic alterations."}
{"id": "PMID:1093227", "title": "Transpyloric extension to duodenal bulb in gastric lymphoma.", "content": "Gastric lymphoma may present in a spectrum of morphologic patterns which may mimic a variety of diseases clinically, endoscopically, radiologically and surgically. From its common location within the gastric antrum, transpyloric submucosal spread frequently occurs to involve the duodenal bulb and occasionally the proximal descending duodenum. This distinctive pathologic characteristic results in contour deformities, filling defects, and ulcerations of the duodenum which can be traced in continuity from the gastric lesions. These radiological observations are significant in the preoperative diagnosis of primary malignant lymphoma of the stomach and in the evaluation of disseminated lymphoma.", "contents": "Transpyloric extension to duodenal bulb in gastric lymphoma. Gastric lymphoma may present in a spectrum of morphologic patterns which may mimic a variety of diseases clinically, endoscopically, radiologically and surgically. From its common location within the gastric antrum, transpyloric submucosal spread frequently occurs to involve the duodenal bulb and occasionally the proximal descending duodenum. This distinctive pathologic characteristic results in contour deformities, filling defects, and ulcerations of the duodenum which can be traced in continuity from the gastric lesions. These radiological observations are significant in the preoperative diagnosis of primary malignant lymphoma of the stomach and in the evaluation of disseminated lymphoma."}
{"id": "PMID:1093228", "title": "Angiographic features of peripancreatic malignant lymphoma.", "content": "Angiographic features are described in 5 patients with peripancreatic lymphomas who initially presented with clinical features of primary pancreatic disease. Each patient demonstrated a large hypovascular mass with considerable displacement of pancreatic and peripancreatic arteries and associated compression of adjacent veins. Smooth arterial encasement was present in 2 cases. Signs of mass out of proportion to signs of invasion mitigated against the diagnosis of carcinoma in each case. Other differential diagnostic considerations are pancreatic pseudocyst and cystadenoma.", "contents": "Angiographic features of peripancreatic malignant lymphoma. Angiographic features are described in 5 patients with peripancreatic lymphomas who initially presented with clinical features of primary pancreatic disease. Each patient demonstrated a large hypovascular mass with considerable displacement of pancreatic and peripancreatic arteries and associated compression of adjacent veins. Smooth arterial encasement was present in 2 cases. Signs of mass out of proportion to signs of invasion mitigated against the diagnosis of carcinoma in each case. Other differential diagnostic considerations are pancreatic pseudocyst and cystadenoma."}
{"id": "PMID:1093229", "title": "Graf/Pen computer for rapid neuroangiographic measurements: Sylvian loops and venous angle.", "content": "The Graf/Pen sonic digitilizer facilitates routine application of neuroradiologic measurements during film interpretation. The Vlahovitch and Probst measurements were performed 23 times faster with this device than manually. The Graf/Pen computer combination shows great potential in the rapid quantitative analysis of both carotid and vertebral angiograms.", "contents": "Graf/Pen computer for rapid neuroangiographic measurements: Sylvian loops and venous angle. The Graf/Pen sonic digitilizer facilitates routine application of neuroradiologic measurements during film interpretation. The Vlahovitch and Probst measurements were performed 23 times faster with this device than manually. The Graf/Pen computer combination shows great potential in the rapid quantitative analysis of both carotid and vertebral angiograms."}
{"id": "PMID:1093230", "title": "Distribution of various labeled thioglucoses in rodents.", "content": "Gold, silver and lead thioglucose were evaluated in rodents to determine the value of organo-metallic compounds in the study of metabolic abnormalities. Increased radioactivity concentration was noted in the hypothalamus with all compounds. Gold thioglucose concentrated in the kidneys and thymus, and 203Pb-thioglucose concentrated in the pancreas and thymus.", "contents": "Distribution of various labeled thioglucoses in rodents. Gold, silver and lead thioglucose were evaluated in rodents to determine the value of organo-metallic compounds in the study of metabolic abnormalities. Increased radioactivity concentration was noted in the hypothalamus with all compounds. Gold thioglucose concentrated in the kidneys and thymus, and 203Pb-thioglucose concentrated in the pancreas and thymus."}
{"id": "PMID:1093241", "title": "The immune response of hens to multiple Escherichia coli injections and transfer of immunoglobulins to the egg and hatched chick.", "content": "Four spaced E coli antigen injections were given to laying hens in order to cause an extended period of antibody production. Antibody could be detected as early as four days after the first antigen injection reaching a peak at the 20th day. IgM was the first antibody to be detected. From the eighth day IgG could be detected replacing the IgM fraction gradually. Antibody to E coli produced by the hen could be detected in yolk of eggs and in chicks from the 15th day but not after the 87th day post treatment. Fluctuation or antibody transmitted by the individual hen was observed. Yolk and chick antibody titres were slightly lower than those found in the serum of the hens on the same day. Only IgG antibodies were found in yolks and serum of day-old chicks.", "contents": "The immune response of hens to multiple Escherichia coli injections and transfer of immunoglobulins to the egg and hatched chick. Four spaced E coli antigen injections were given to laying hens in order to cause an extended period of antibody production. Antibody could be detected as early as four days after the first antigen injection reaching a peak at the 20th day. IgM was the first antibody to be detected. From the eighth day IgG could be detected replacing the IgM fraction gradually. Antibody to E coli produced by the hen could be detected in yolk of eggs and in chicks from the 15th day but not after the 87th day post treatment. Fluctuation or antibody transmitted by the individual hen was observed. Yolk and chick antibody titres were slightly lower than those found in the serum of the hens on the same day. Only IgG antibodies were found in yolks and serum of day-old chicks."}
{"id": "PMID:1093242", "title": "Experimental infection of cockerels with Salmonella typhimurium.", "content": "Six-month-old SPF Brown Leghorn cockerels were experimentally infected per os with S typhimurium (1 times 1010) and slaughtered at intervals up to 42 days following infection. Observations were made on the clinical pathology, bacteriology and serology during the course of infection and extensive bacteriological examinations were undertaken after post mortem examination.", "contents": "Experimental infection of cockerels with Salmonella typhimurium. Six-month-old SPF Brown Leghorn cockerels were experimentally infected per os with S typhimurium (1 times 1010) and slaughtered at intervals up to 42 days following infection. Observations were made on the clinical pathology, bacteriology and serology during the course of infection and extensive bacteriological examinations were undertaken after post mortem examination."}
{"id": "PMID:1093243", "title": "Immune response of sheep to Fasciola hepatica infection.", "content": "The IgG immunoglobulin levels and the immune response to Fasciola hepatica digestive tract antigens were studied in experimentally infected lambs. A synchronic increase and decrease of IgG-1 serum levels and indirect fluorescent antibody titres were observed during the course of the infection. Antibody activities were demonstrated in IgG-1, but not in IgG-2 isolates. The antibody activity was partly sensitive to reduction with mercaptoethanol.", "contents": "Immune response of sheep to Fasciola hepatica infection. The IgG immunoglobulin levels and the immune response to Fasciola hepatica digestive tract antigens were studied in experimentally infected lambs. A synchronic increase and decrease of IgG-1 serum levels and indirect fluorescent antibody titres were observed during the course of the infection. Antibody activities were demonstrated in IgG-1, but not in IgG-2 isolates. The antibody activity was partly sensitive to reduction with mercaptoethanol."}
{"id": "PMID:1093244", "title": "Immunoglobulin classes of antibodies produced against Toxoplasma gondii by ovine fetuses.", "content": "Sera from aborted or newborn lambs with congenital toxoplasmosis were fractionated on Sephadex G-200. Sera and fractions were tested for Toxoplasma antibodies using the indirect fluorescent antibody test. Most of the antibody was found to reside in the IgG fraction.", "contents": "Immunoglobulin classes of antibodies produced against Toxoplasma gondii by ovine fetuses. Sera from aborted or newborn lambs with congenital toxoplasmosis were fractionated on Sephadex G-200. Sera and fractions were tested for Toxoplasma antibodies using the indirect fluorescent antibody test. Most of the antibody was found to reside in the IgG fraction."}
{"id": "PMID:1093255", "title": "Megakaryocytes in agar cultures of mouse bone marrow.", "content": "Cytologic and immunofluorescent data demonstrate that cells morphologically resembling megakaryocytes appear in thin layer agar cultures of cellular fractions of mouse bone marrow and increase in number from zero to a maximum of approxiamately 60 per culture on day 6. It would appear that these \"candidate\" megakaryocytes are derived from a morphologically unidentified precursor(s) since they are not present in the original cell suspension cultured but begin to appear 24 hours later. Similarities between the ultrastructure of \"candidate\" megakaryocytes and that of femoral marrow megakaryocytes support morphological similarities at the light microscopic level. Pretreatment of mice with either vinblastine and nitrogen mustard or antiplatelet serum has no effect on the number of \"candidate\" megakaryocytes appearing in cultures of their marrow. Preliminary data suggest that the rate of appearance of these cells in culture is responsive to serum from thrombocytopenic donors.", "contents": "Megakaryocytes in agar cultures of mouse bone marrow. Cytologic and immunofluorescent data demonstrate that cells morphologically resembling megakaryocytes appear in thin layer agar cultures of cellular fractions of mouse bone marrow and increase in number from zero to a maximum of approxiamately 60 per culture on day 6. It would appear that these \"candidate\" megakaryocytes are derived from a morphologically unidentified precursor(s) since they are not present in the original cell suspension cultured but begin to appear 24 hours later. Similarities between the ultrastructure of \"candidate\" megakaryocytes and that of femoral marrow megakaryocytes support morphological similarities at the light microscopic level. Pretreatment of mice with either vinblastine and nitrogen mustard or antiplatelet serum has no effect on the number of \"candidate\" megakaryocytes appearing in cultures of their marrow. Preliminary data suggest that the rate of appearance of these cells in culture is responsive to serum from thrombocytopenic donors."}
{"id": "PMID:1093259", "title": "Use of blood and blood components.", "content": "The indications for transfusions are anemia compromising delivery of oxygen, acute blood loss, cardiopulmonary bypass, exchange transfusion, maintenance of hemostasis, and sepsis associated with granulocytopenia. When transfusion therapy is indicated, only that component of whole blood which is needed for correction of the problem should be given. The options for use each component have been discussed.", "contents": "Use of blood and blood components. The indications for transfusions are anemia compromising delivery of oxygen, acute blood loss, cardiopulmonary bypass, exchange transfusion, maintenance of hemostasis, and sepsis associated with granulocytopenia. When transfusion therapy is indicated, only that component of whole blood which is needed for correction of the problem should be given. The options for use each component have been discussed."}
{"id": "PMID:1093266", "title": "Pancreatic pseudocysts.", "content": "Pseudocysts are fascinating lesions of the pancreas which present as upper abdominal masses in about 80 per cent of cases, but also as obstructive jaundice, intrasplenic and intra-left-renal masses, mediastinal masses, flank abscess, pleural effusions, and ascites rich in amylase. They are diagnosed by urine amylase with greater sensitivity than by serum amylase. Cysts must be differentiated from pancreatic abscesses, indolent phlegmonous pancreatitis, cystadenomas, and carcinomas of the pancreas, as well as lymphomas and other rare tumors. They may be satisfactorily treated by large sump tube drains to the outside but about one third will require a further operation. Internal drainage by cystogastrostomy is the simplest and best current procedure, but not applicable to all cases. A good alternative is Roux-en-y cyst-jejunostomy. Caution is needed in the preoperative workup since all procedures are accompanied by an operative mortality approaching 10 per cent, mostly due to hepatic disease, portal hypertension, stress ulcerations, and hemorrhage. The follow-up shows 85 per cent good results from proper drainage but 40 per cent are diabetics and in many patients the ravages of alcoholism continue unabated.", "contents": "Pancreatic pseudocysts. Pseudocysts are fascinating lesions of the pancreas which present as upper abdominal masses in about 80 per cent of cases, but also as obstructive jaundice, intrasplenic and intra-left-renal masses, mediastinal masses, flank abscess, pleural effusions, and ascites rich in amylase. They are diagnosed by urine amylase with greater sensitivity than by serum amylase. Cysts must be differentiated from pancreatic abscesses, indolent phlegmonous pancreatitis, cystadenomas, and carcinomas of the pancreas, as well as lymphomas and other rare tumors. They may be satisfactorily treated by large sump tube drains to the outside but about one third will require a further operation. Internal drainage by cystogastrostomy is the simplest and best current procedure, but not applicable to all cases. A good alternative is Roux-en-y cyst-jejunostomy. Caution is needed in the preoperative workup since all procedures are accompanied by an operative mortality approaching 10 per cent, mostly due to hepatic disease, portal hypertension, stress ulcerations, and hemorrhage. The follow-up shows 85 per cent good results from proper drainage but 40 per cent are diabetics and in many patients the ravages of alcoholism continue unabated."}
{"id": "PMID:1093270", "title": "Donor heart resuscitation and storage.", "content": "At the present time, simple hypothermia or regional or total body perfusion probably afford the best means of myocardial protection of the donor heart. However, the potential of either technique is extremely limited, and a combination of hypothermia with some form of perfusion system will probably enable considerably longer periods of storage of the donor organ. Such a perfusion system has not yet been conclusively developed, though considerable advances have been made. It is doubtful whether or not the addition of hyperbaric oxygen to hypothermia greatly prolongs the storage period. Metabolic inhibition by a chemical agent is an attractive method of preservation, possibly associated with hypothermia, but the search for the perfect agent continues. Actual freezing of the organ may prove feasible in the future, but recent work in the field of cryobiology has proved almost uniformly disappointing. The use of the autoperfusing heart-lung preparation as a short term storage system deserves further study, but its value as a really long term system of storage of the heart seems unlikely at the present time. Xenobanking has been encouragingly successful in experimental situations, but its clinical application will prove to be expensive and difficult.", "contents": "Donor heart resuscitation and storage. At the present time, simple hypothermia or regional or total body perfusion probably afford the best means of myocardial protection of the donor heart. However, the potential of either technique is extremely limited, and a combination of hypothermia with some form of perfusion system will probably enable considerably longer periods of storage of the donor organ. Such a perfusion system has not yet been conclusively developed, though considerable advances have been made. It is doubtful whether or not the addition of hyperbaric oxygen to hypothermia greatly prolongs the storage period. Metabolic inhibition by a chemical agent is an attractive method of preservation, possibly associated with hypothermia, but the search for the perfect agent continues. Actual freezing of the organ may prove feasible in the future, but recent work in the field of cryobiology has proved almost uniformly disappointing. The use of the autoperfusing heart-lung preparation as a short term storage system deserves further study, but its value as a really long term system of storage of the heart seems unlikely at the present time. Xenobanking has been encouragingly successful in experimental situations, but its clinical application will prove to be expensive and difficult."}
{"id": "PMID:1093271", "title": "Re-evaluation of the biomechanics of blunt impact injury of the head.", "content": "With blunt impacts, there may be bruising or tearing of scalp, elastic deformation of the skull with or without linear fracture, mass motions or relative movements of the brain mass--inertial stress propagation--as well as pressure gradients. Mass motions of the brain may cause concussion, vascular tears with extravasation and contusions of the cerebral surface as well as lesions in the deeper portions of the cerebral mass. Coup contusion is caused by the inbending bone at the impact site striking the immobile head. Contrecoup contusiions result from injury to the brain surface in the frontotemporal area as the moving brain impacts the rough and irregular bony interior. In the experimental laboratory, a contusion is easily produced by a moving object striking the relatively immobile head. During impact if the head is on a solid stand, such as the metal table, a contrecoup lesion is seen at the opposite end from the impact site. This contrecoup lesion is produced by the brain impacting the skull, as well as the flattening of the skull against the brain at the antipole, from the presence of the solid table. Intermediate contusions develop between the impact site and the antipole in the brain interior as a result of inertial stress propagation.", "contents": "Re-evaluation of the biomechanics of blunt impact injury of the head. With blunt impacts, there may be bruising or tearing of scalp, elastic deformation of the skull with or without linear fracture, mass motions or relative movements of the brain mass--inertial stress propagation--as well as pressure gradients. Mass motions of the brain may cause concussion, vascular tears with extravasation and contusions of the cerebral surface as well as lesions in the deeper portions of the cerebral mass. Coup contusion is caused by the inbending bone at the impact site striking the immobile head. Contrecoup contusiions result from injury to the brain surface in the frontotemporal area as the moving brain impacts the rough and irregular bony interior. In the experimental laboratory, a contusion is easily produced by a moving object striking the relatively immobile head. During impact if the head is on a solid stand, such as the metal table, a contrecoup lesion is seen at the opposite end from the impact site. This contrecoup lesion is produced by the brain impacting the skull, as well as the flattening of the skull against the brain at the antipole, from the presence of the solid table. Intermediate contusions develop between the impact site and the antipole in the brain interior as a result of inertial stress propagation."}
{"id": "PMID:1093272", "title": "Direct influence of endotoxin on cellular respiration.", "content": "Hepatocyte oxygen consumption was evaluated in vitro in 70 rabbits. In selected instances, Escherichia coli endotoxin was added to a chamber containing either rabbit hepatocytes or hepatocyte homogenates. Endotoxin directly depressed the respiration of intact rabbit hepatocytes. Endotoxin also decreased the oxygen consumption of hepatocyte homogenates. The intact cell membrane is not an effective barrier to the deleterious effect of endotoxin. A patient in a state of endotoxic shock, in addition to having a hemodynamic derangement, probably has circulating toxins that directly render cellular oxidative phsophorylation less efficient.", "contents": "Direct influence of endotoxin on cellular respiration. Hepatocyte oxygen consumption was evaluated in vitro in 70 rabbits. In selected instances, Escherichia coli endotoxin was added to a chamber containing either rabbit hepatocytes or hepatocyte homogenates. Endotoxin directly depressed the respiration of intact rabbit hepatocytes. Endotoxin also decreased the oxygen consumption of hepatocyte homogenates. The intact cell membrane is not an effective barrier to the deleterious effect of endotoxin. A patient in a state of endotoxic shock, in addition to having a hemodynamic derangement, probably has circulating toxins that directly render cellular oxidative phsophorylation less efficient."}
{"id": "PMID:1093273", "title": "Trends in surgical treatment of calculous disease of the biliary tract.", "content": "From the data presented, there are five distinct trends in operations for calculous biliary tract disease. There is less delay in operation following diagnosis than ever before. Diagnosis is being made early in those with symptoms. Asymptomatic calculi are being revealed more frequently by complete over-all medical evaluations including cholecystography. Acute obstructive cholecystitis, a complication of cholelithiasis, is becoming less frequent in proportion to the total patients admitted to hsopitals. The operative mortality is concentrated among those in the elderly group, 65 years old and older. The limited capacity of many patients in this category to tolerate the burden of any surgical procedure is being met by palliative, temporary procedures such as cholecystostomy and ductal decompression rather than definitive cholecystectomy and choledochotomy. Roentgenologic facilities are becoming available to provide visualization and critical evaluation of the entire ductal system at the beginning of an operation. This provides a more realistic basis for common duct exploration, an increment associated with morbidity. More important is the assurance against overlooking calculi, retained ductal stones.", "contents": "Trends in surgical treatment of calculous disease of the biliary tract. From the data presented, there are five distinct trends in operations for calculous biliary tract disease. There is less delay in operation following diagnosis than ever before. Diagnosis is being made early in those with symptoms. Asymptomatic calculi are being revealed more frequently by complete over-all medical evaluations including cholecystography. Acute obstructive cholecystitis, a complication of cholelithiasis, is becoming less frequent in proportion to the total patients admitted to hsopitals. The operative mortality is concentrated among those in the elderly group, 65 years old and older. The limited capacity of many patients in this category to tolerate the burden of any surgical procedure is being met by palliative, temporary procedures such as cholecystostomy and ductal decompression rather than definitive cholecystectomy and choledochotomy. Roentgenologic facilities are becoming available to provide visualization and critical evaluation of the entire ductal system at the beginning of an operation. This provides a more realistic basis for common duct exploration, an increment associated with morbidity. More important is the assurance against overlooking calculi, retained ductal stones."}
{"id": "PMID:1093281", "title": "[A clinico-morphologico-functional study of the kidneys in glomerulonephritis].", "content": "The article deals with the results of a clinico-functional-morphological study of the kidneys in glomerulonephritis (50 observations) which was carried out with the use of the method of puncture biopsy of the kidneys. It was shown that clinical forms of glomerulonephritis, accompanied with hematuria, were characterized by the absence of fixation of immune complexes in the basal membrane of the glomeruli. According to the electron microscopy data, this corresponds to the dissappearance of deposits from the subendothelial parts of the basal membrane. The extramembranous glomerulonephritis, revealed with the help of electron microscopy technique, was characterized by the nephrotic syndrome. An increased tension of immunological processes in the glomeruli was accompanied by a more grave clinical course of glomerulonephritis, by impairment of not only glomerulous but tubular functions as well. A greater dicrease in the function of osmotic concentration of the urine in fibroplastic types of glomerulonephritis, as compared with non-firboplastic ones, was apparently connected not only with lesiones of tubules but rather with more expressed sclerotic changes in the interstitial tissue of the kidney.", "contents": "[A clinico-morphologico-functional study of the kidneys in glomerulonephritis]. The article deals with the results of a clinico-functional-morphological study of the kidneys in glomerulonephritis (50 observations) which was carried out with the use of the method of puncture biopsy of the kidneys. It was shown that clinical forms of glomerulonephritis, accompanied with hematuria, were characterized by the absence of fixation of immune complexes in the basal membrane of the glomeruli. According to the electron microscopy data, this corresponds to the dissappearance of deposits from the subendothelial parts of the basal membrane. The extramembranous glomerulonephritis, revealed with the help of electron microscopy technique, was characterized by the nephrotic syndrome. An increased tension of immunological processes in the glomeruli was accompanied by a more grave clinical course of glomerulonephritis, by impairment of not only glomerulous but tubular functions as well. A greater dicrease in the function of osmotic concentration of the urine in fibroplastic types of glomerulonephritis, as compared with non-firboplastic ones, was apparently connected not only with lesiones of tubules but rather with more expressed sclerotic changes in the interstitial tissue of the kidney."}
{"id": "PMID:1093284", "title": "Improved HL-A typing of cadaveric renal donors. I. Quantitation of HL-A gene dosage.", "content": "A quantitative microabsorption technique has been used to determine a gene dosage effect on HL-A. This allows homozygosity or heterozygosity of HL-A antigens to be determined prospectively in cadaver donors of renal allografts where less than four antigens were detected by standard lymphocytotoxicity. This in turn provides more precise matching of donor and recipient.", "contents": "Improved HL-A typing of cadaveric renal donors. I. Quantitation of HL-A gene dosage. A quantitative microabsorption technique has been used to determine a gene dosage effect on HL-A. This allows homozygosity or heterozygosity of HL-A antigens to be determined prospectively in cadaver donors of renal allografts where less than four antigens were detected by standard lymphocytotoxicity. This in turn provides more precise matching of donor and recipient."}
{"id": "PMID:1093285", "title": "Reactions of kidney cells with cytotoxic antisera: possible evidence of kidney-specific antigens.", "content": "HL-A typing of cadaver kidney cell suspension by fluorochromasia cytotoxicity was successful in 76 out of 124 cases. In the 44 cases with confirmed phenotypes, 24 had identical results for lymphocytes and kidney cells. Twenty kidney cells had HL-A antigens not detected on that donor's lymphocytes, most commonly HL-A7 and HL-A8. In eight of these 20 cases, the additional kidney antigens brought the total to more than two per segregant sereis, in disagreement with an earlier report from this laboratory. The discrepancy was traced to a change in the method of complement preparation. The complement was successfully deprived of the resulting non-specific cytotoxicity for kidney cells by absorption with human red blood cells. Prior to absorption, the complement had rendered kidney cells susceptible to the lytic effect of anti-A and B red cell antibodies. Using the absorbed complement, a patient who had hyperacutely rejected two cadaver kidneys provided sera with an antibody reacting with, and absorbed by, the kidney cells but not the lymphocytes of the donors.", "contents": "Reactions of kidney cells with cytotoxic antisera: possible evidence of kidney-specific antigens. HL-A typing of cadaver kidney cell suspension by fluorochromasia cytotoxicity was successful in 76 out of 124 cases. In the 44 cases with confirmed phenotypes, 24 had identical results for lymphocytes and kidney cells. Twenty kidney cells had HL-A antigens not detected on that donor's lymphocytes, most commonly HL-A7 and HL-A8. In eight of these 20 cases, the additional kidney antigens brought the total to more than two per segregant sereis, in disagreement with an earlier report from this laboratory. The discrepancy was traced to a change in the method of complement preparation. The complement was successfully deprived of the resulting non-specific cytotoxicity for kidney cells by absorption with human red blood cells. Prior to absorption, the complement had rendered kidney cells susceptible to the lytic effect of anti-A and B red cell antibodies. Using the absorbed complement, a patient who had hyperacutely rejected two cadaver kidneys provided sera with an antibody reacting with, and absorbed by, the kidney cells but not the lymphocytes of the donors."}
{"id": "PMID:1093287", "title": "Mycoplasma in synovial fluid from the patients with rheumatoid arthritis.", "content": "Mycoplasma colonies were found on culture of synovial fluid in 14 of 18 cases of rheumatoid arthritis. It was possible to demonstrate the colonies in primary culture medium only by the method of hot water fixation and Giemsa staining. The organisms grown on solid medium were also observed electron-microscopically.", "contents": "Mycoplasma in synovial fluid from the patients with rheumatoid arthritis. Mycoplasma colonies were found on culture of synovial fluid in 14 of 18 cases of rheumatoid arthritis. It was possible to demonstrate the colonies in primary culture medium only by the method of hot water fixation and Giemsa staining. The organisms grown on solid medium were also observed electron-microscopically."}
{"id": "PMID:1093288", "title": "Amino acid metabolism in Wegener's granulomatosis.", "content": "The urinary excretion of amino acids and their derivatives in cases of Wegener's granulomatosis have been examined, and the excretion of urinary amino acids in such cases was different from normal and reticulum cell sarcoma. In Wegener's cases, the amino acids which were always found in readily detectable amounts totaled 33; urinary phenylalanine was 4-5 times higher than in normal subjects. From these experimental results, we believe that the observation of amino acid excretion into urine is an important indicator for the diagnosis of Wegener's granulomatosis. In addition, the results of quantitative examination on the urinary amino acid excretion were compared with normal and reticulum cell sarcoma. Comparison of the analytical data on urinary amino acids of the normal and patient groups indicated significant metabolic abnormalities of some amino acids at the earliest stage of Wegener's granulomatosis.", "contents": "Amino acid metabolism in Wegener's granulomatosis. The urinary excretion of amino acids and their derivatives in cases of Wegener's granulomatosis have been examined, and the excretion of urinary amino acids in such cases was different from normal and reticulum cell sarcoma. In Wegener's cases, the amino acids which were always found in readily detectable amounts totaled 33; urinary phenylalanine was 4-5 times higher than in normal subjects. From these experimental results, we believe that the observation of amino acid excretion into urine is an important indicator for the diagnosis of Wegener's granulomatosis. In addition, the results of quantitative examination on the urinary amino acid excretion were compared with normal and reticulum cell sarcoma. Comparison of the analytical data on urinary amino acids of the normal and patient groups indicated significant metabolic abnormalities of some amino acids at the earliest stage of Wegener's granulomatosis."}
{"id": "PMID:1093301", "title": "Instrument to facilitate nephrostomy.", "content": "An instrument to facilitate the placement of a nephrostomy tube is described. The techniques for performing nephrostomies are historically reviewed.", "contents": "Instrument to facilitate nephrostomy. An instrument to facilitate the placement of a nephrostomy tube is described. The techniques for performing nephrostomies are historically reviewed."}
{"id": "PMID:1093302", "title": "Kidney cooling in partial nephrectomy. Evaluation of a technique.", "content": "A technique of cooling the isolated human kidney by immersing it in a plastic bag filled with iced saline slush is described. This technique is simple, swift to perform, requires no specialized equipment, and is inexpensive; it has proved to be effective and safe.", "contents": "Kidney cooling in partial nephrectomy. Evaluation of a technique. A technique of cooling the isolated human kidney by immersing it in a plastic bag filled with iced saline slush is described. This technique is simple, swift to perform, requires no specialized equipment, and is inexpensive; it has proved to be effective and safe."}
{"id": "PMID:1093303", "title": "Small-Carrion penile prosthesis. New implant for management of impotence.", "content": "The etiology of impotence and a review of various techniques in treatment are discussed. A perineal surgical approach with a new type of paired sponge-filled silicone prostheses is described. The prostheses are inserted into previously dilated corpora cavernosa. Of the initial 31 patients, excellent results were obtained 27, and a good result in 1. Of the 3 patients with initial serious complications, adequate functional results were attained in 2. The distinct advantages and potential complications using the Small-Carrion penile prosthesis and surgical technique are discussed.", "contents": "Small-Carrion penile prosthesis. New implant for management of impotence. The etiology of impotence and a review of various techniques in treatment are discussed. A perineal surgical approach with a new type of paired sponge-filled silicone prostheses is described. The prostheses are inserted into previously dilated corpora cavernosa. Of the initial 31 patients, excellent results were obtained 27, and a good result in 1. Of the 3 patients with initial serious complications, adequate functional results were attained in 2. The distinct advantages and potential complications using the Small-Carrion penile prosthesis and surgical technique are discussed."}
{"id": "PMID:1093306", "title": "Blood urea nitrogen and serum creatinine.  Physiology and interpretations.", "content": "Any elevations in levels of blood urea nitrogen and/or serum creatinine do not necessarily indicate structural renal disease.  Conversely, blood urea nitrogen or serum creatinine values, which appear to be within the range of normal, do not by themselves rule out significant reduction in glomerular filtration rate.  Any interpretation of the blood levels of these two substances must be done with the awareness that a variety of extrarenal factors can affect them.  The blood urea nitrogen to serum creatinine ratio can be a valuable tool in the determination or renal functional and structural integrity.", "contents": "Blood urea nitrogen and serum creatinine.  Physiology and interpretations. Any elevations in levels of blood urea nitrogen and/or serum creatinine do not necessarily indicate structural renal disease.  Conversely, blood urea nitrogen or serum creatinine values, which appear to be within the range of normal, do not by themselves rule out significant reduction in glomerular filtration rate.  Any interpretation of the blood levels of these two substances must be done with the awareness that a variety of extrarenal factors can affect them.  The blood urea nitrogen to serum creatinine ratio can be a valuable tool in the determination or renal functional and structural integrity."}
{"id": "PMID:1093307", "title": "Hematologic problems in urology. Basic mechanisms and clinical approach.", "content": "Baxic clotting mechanisms are reviewed. The clinical and laboratory aspects of disseminated intravascular coagulation and fibrinolysis are discussed, and an approach is suggested to those problems encountered by the urologist.", "contents": "Hematologic problems in urology. Basic mechanisms and clinical approach. Baxic clotting mechanisms are reviewed. The clinical and laboratory aspects of disseminated intravascular coagulation and fibrinolysis are discussed, and an approach is suggested to those problems encountered by the urologist."}
{"id": "PMID:1093308", "title": "Urinary tract disorders.  Clinical comparison of flavoxate and phenazopyridine.", "content": "In nine separate clinical trials, 382 patients having symptoms of either prostatitis, acute cystitis, urethritis, and/or trigonitis were randomly assigned to treatment with flavoxate or phenazopyridine.  Over-all response was evaluated in 384 patients after five days of therapy.  In patients having prostatitis, response was satisfactory in 66 per cent treated with flavoxate and 31 per cent treated with phenazopyridine.  In all other patients, satisfactory responses were reported in 80 per cent on flavoxate compared with 56 per cent on phenazopyridine.  Similarly, symptom-severity evaluations at two and five days of therapy showed most symptoms improved in more of the patients on flavoxate therapy than on phenazopyridine therapy.  Although more adverse effects were reported in patients treated with phenazopyridine than with flavoxate, the difference between medications was not statistically significant.", "contents": "Urinary tract disorders.  Clinical comparison of flavoxate and phenazopyridine. In nine separate clinical trials, 382 patients having symptoms of either prostatitis, acute cystitis, urethritis, and/or trigonitis were randomly assigned to treatment with flavoxate or phenazopyridine.  Over-all response was evaluated in 384 patients after five days of therapy.  In patients having prostatitis, response was satisfactory in 66 per cent treated with flavoxate and 31 per cent treated with phenazopyridine.  In all other patients, satisfactory responses were reported in 80 per cent on flavoxate compared with 56 per cent on phenazopyridine.  Similarly, symptom-severity evaluations at two and five days of therapy showed most symptoms improved in more of the patients on flavoxate therapy than on phenazopyridine therapy.  Although more adverse effects were reported in patients treated with phenazopyridine than with flavoxate, the difference between medications was not statistically significant."}
{"id": "PMID:1093309", "title": "Treatment of chronic prostatitis.  Comparison of Minocycline and Doxycycline.", "content": "The results of minocycline and doxycycline therapy in 41 patients with chronic prostatitis and minocycline therapy in 6 patients with acute prostatitis were evaluated.  In the comparative study of chronic prostatitis, minocycline and doxycycline were given on the same dosage schedule, milligram for milligram: a loading dose of 200 mg. followed by 100 mg. twicd daily.  Over-all clinical responses to therapy with either agent were generally satisfactory, and no statistically significant difference was demonstrable in this regard.  In the group with chronic prostatitis treated wtih minocycline, however, all symptoms manifested before therapy had disappeared after therapy even where over-all results had been judged unsatisfactory.  This was not true of the group with chronic prostatitis treated with doxycycline.  Symptoms in 6 patients persisted after therapy had been terminated.  Here the difference in results between the two groups was found to be statistically significant.  A review of symptoms two to eight weeks after therapy revealed no significant difference between the two groups. After two years only 6 patients in the entire group with chronic prostatitis had returned with recurrent problems: 3 of these patients had been treated with minocycline and 3 had been treated with doxycycline.  Results of therapy in the small series of patients with acute prostatitis treated with minocycline were generally satisfactory.", "contents": "Treatment of chronic prostatitis.  Comparison of Minocycline and Doxycycline. The results of minocycline and doxycycline therapy in 41 patients with chronic prostatitis and minocycline therapy in 6 patients with acute prostatitis were evaluated.  In the comparative study of chronic prostatitis, minocycline and doxycycline were given on the same dosage schedule, milligram for milligram: a loading dose of 200 mg. followed by 100 mg. twicd daily.  Over-all clinical responses to therapy with either agent were generally satisfactory, and no statistically significant difference was demonstrable in this regard.  In the group with chronic prostatitis treated wtih minocycline, however, all symptoms manifested before therapy had disappeared after therapy even where over-all results had been judged unsatisfactory.  This was not true of the group with chronic prostatitis treated with doxycycline.  Symptoms in 6 patients persisted after therapy had been terminated.  Here the difference in results between the two groups was found to be statistically significant.  A review of symptoms two to eight weeks after therapy revealed no significant difference between the two groups. After two years only 6 patients in the entire group with chronic prostatitis had returned with recurrent problems: 3 of these patients had been treated with minocycline and 3 had been treated with doxycycline.  Results of therapy in the small series of patients with acute prostatitis treated with minocycline were generally satisfactory."}
{"id": "PMID:1093310", "title": "Bacterial effect on sperm motility.", "content": "Human semen containing normal number of sperm was exposed to concentrations of Escherichia coli varying from 500 to 10-8 colonies per cubic centimeter.  A significant decrease in motility was abserved at 10-6 colonies per cubic centimeter.", "contents": "Bacterial effect on sperm motility. Human semen containing normal number of sperm was exposed to concentrations of Escherichia coli varying from 500 to 10-8 colonies per cubic centimeter.  A significant decrease in motility was abserved at 10-6 colonies per cubic centimeter."}
{"id": "PMID:1093324", "title": "[Use of ftorafur for the treatment of 3d-4th stage].", "content": "To treat ovarian cancer of stage III-IV the authors have used an antimetabolite-fluorafur. A total dosage of 17-23 g. \"marked improvement\" was noted in 15 patients, \"insignificant improvement\" - in 9, there was no improvement in one case. It was found that the level of FSH excretion was reduced twice under the influence of fluorafur, the greater decrease corresponding to a better clinical effect. The drug used in doses applied by the authors, proved to be nontoxic.", "contents": "[Use of ftorafur for the treatment of 3d-4th stage]. To treat ovarian cancer of stage III-IV the authors have used an antimetabolite-fluorafur. A total dosage of 17-23 g. \"marked improvement\" was noted in 15 patients, \"insignificant improvement\" - in 9, there was no improvement in one case. It was found that the level of FSH excretion was reduced twice under the influence of fluorafur, the greater decrease corresponding to a better clinical effect. The drug used in doses applied by the authors, proved to be nontoxic."}
{"id": "PMID:1093327", "title": "Orthopedic applications of liquid crystal thermography.", "content": "Liquid crystal thermography can assist the clinician in evaluating inflammatory conditions or delineating viability of tissues by the differences in coloration that occur with the use of these unique substances. The technique has proved useful in studying hemophilia, tumors of the extremities, arthritis and vascular conditions, including the complications of diabetes.", "contents": "Orthopedic applications of liquid crystal thermography. Liquid crystal thermography can assist the clinician in evaluating inflammatory conditions or delineating viability of tissues by the differences in coloration that occur with the use of these unique substances. The technique has proved useful in studying hemophilia, tumors of the extremities, arthritis and vascular conditions, including the complications of diabetes."}
{"id": "PMID:1093330", "title": "[The action of a tropic acid ester on bronchospasm: a double-blind study (author's transl)].", "content": "12 patients with chronic bronchitis were treated with two different doses (0.02 and 0.08 mg) of Sch 1000 (Atrovent) administered by metered dose inhalation and the results compared with those following administration of a placebo in a cross-over double-blind study. The airway resistance and thoracic gas volume fell significantly after treatment with the drug, with no appreciable difference in effect between the dosages. The bronchodilatory action of the drug lasted about 4 hours and differed markedly from that of the placebo. No anticholinergic side effects or specific effects on the circulation were encountered at the administered dosage.", "contents": "[The action of a tropic acid ester on bronchospasm: a double-blind study (author's transl)]. 12 patients with chronic bronchitis were treated with two different doses (0.02 and 0.08 mg) of Sch 1000 (Atrovent) administered by metered dose inhalation and the results compared with those following administration of a placebo in a cross-over double-blind study. The airway resistance and thoracic gas volume fell significantly after treatment with the drug, with no appreciable difference in effect between the dosages. The bronchodilatory action of the drug lasted about 4 hours and differed markedly from that of the placebo. No anticholinergic side effects or specific effects on the circulation were encountered at the administered dosage."}
{"id": "PMID:1093342", "title": "[Chromosome studies on human fibroblast cultures following exposure to cyproheptadin].", "content": "Cyproheptadin was tested on chromosomes in fibroblast cultures of children for its possible mutagenic efficacy. Neither 24-hour exposure to Cyproheptadin at concentrations of 8-16 mg/l medium nor a chronic exposure for 8-11 weeks at a concentration of 8 mg/l caused any discernable chromosomal mutations. At concentrations between 8-16 mg/l reversible cytomorphological and cytochemical changes occured; 32 mg/l suppressed cell division and higher concentrations (64-128 mg/l) brought about irreversible changes leading to cell death. These reactions were rather in vitro cytotoxic effects of unspecific nature.", "contents": "[Chromosome studies on human fibroblast cultures following exposure to cyproheptadin]. Cyproheptadin was tested on chromosomes in fibroblast cultures of children for its possible mutagenic efficacy. Neither 24-hour exposure to Cyproheptadin at concentrations of 8-16 mg/l medium nor a chronic exposure for 8-11 weeks at a concentration of 8 mg/l caused any discernable chromosomal mutations. At concentrations between 8-16 mg/l reversible cytomorphological and cytochemical changes occured; 32 mg/l suppressed cell division and higher concentrations (64-128 mg/l) brought about irreversible changes leading to cell death. These reactions were rather in vitro cytotoxic effects of unspecific nature."}
{"id": "PMID:1093343", "title": "An in vivo and in vitro phosphorylation of yeast ribosomal proteins.", "content": "Phosphorylation of yeast ribosomal proteins has been demonstrated in vivo and in vitro. 32-P-labelled product represents an ester-linked class of phosphoprotein. Acrylamide-gel electrophoresis has shown that in both types of experiments radioactive proteins migrate similarly; this might indicate that closely related groups of proteins become phosphorylated in vivo and in vitro. In the presence of [32-P] ATP the amount of covalently bound phosphate was 1.0 - 1.2 moles/mole of ribosome. The phosphorylation of ribosomal proteins did not appreciably affect the activity of ribosomes in a cell-free protein-synthesizing system containing poly(U) and elongation factors.", "contents": "An in vivo and in vitro phosphorylation of yeast ribosomal proteins. Phosphorylation of yeast ribosomal proteins has been demonstrated in vivo and in vitro. 32-P-labelled product represents an ester-linked class of phosphoprotein. Acrylamide-gel electrophoresis has shown that in both types of experiments radioactive proteins migrate similarly; this might indicate that closely related groups of proteins become phosphorylated in vivo and in vitro. In the presence of [32-P] ATP the amount of covalently bound phosphate was 1.0 - 1.2 moles/mole of ribosome. The phosphorylation of ribosomal proteins did not appreciably affect the activity of ribosomes in a cell-free protein-synthesizing system containing poly(U) and elongation factors."}
{"id": "PMID:1093345", "title": "Preparative enzymic synthesis of nucleoside-5'-phosphates.", "content": "1. Wheat shoot phosphotransferase has been employed, with p-nitrophenylphosphate as a phosphate donor, to specifically phosphorylate the 5'-position of a variety of nucleosides and nucleoside analogues. The specificity of the enzyme towards the 5'-position of pentose nucleosides is testified to by the complete resistance to phosphorylation of 5'-O-methylcytidine. 2. With the use of ion-exchange chromatography, the foregoing procedure has been applied to the large-scale preparation of nucleoside-5'-phosphates with overall yields of the order of 80-90%. Quantitative recovery of unreacted nucleoside makes it possible to use this method without risk of losses either on a small or large scale with rare nucleosides. It is also applicable to acid- and alkali-labile nucleosides which cannot readily be phosphorylated by chemical procedures. 3. The wheat shoot phosphotransferase also phosphorylated a galactopyranosyl nucleoside, as well as such derivatives as 1-(beta-hydroxyethyl)cytosine and 5-(beta-hydroxyethyl)uracil, showing that the enzyme does not have an absolute requirement for a 5-membered sugar ring, but rather for the presence of a primary hydroxyl group. 4. The phosphorylated derivatives of galactopyranosyluracil, and of both hydroxyethyl pyrimidines, were resistant to 5'-nucleotidase. E. coli alkaline phosphatase converted all three nucleotides quantitatively to the starting compounds. 5. A synthesis of 1-(beta-hydroxyethyl)cytosine is described.", "contents": "Preparative enzymic synthesis of nucleoside-5'-phosphates. 1. Wheat shoot phosphotransferase has been employed, with p-nitrophenylphosphate as a phosphate donor, to specifically phosphorylate the 5'-position of a variety of nucleosides and nucleoside analogues. The specificity of the enzyme towards the 5'-position of pentose nucleosides is testified to by the complete resistance to phosphorylation of 5'-O-methylcytidine. 2. With the use of ion-exchange chromatography, the foregoing procedure has been applied to the large-scale preparation of nucleoside-5'-phosphates with overall yields of the order of 80-90%. Quantitative recovery of unreacted nucleoside makes it possible to use this method without risk of losses either on a small or large scale with rare nucleosides. It is also applicable to acid- and alkali-labile nucleosides which cannot readily be phosphorylated by chemical procedures. 3. The wheat shoot phosphotransferase also phosphorylated a galactopyranosyl nucleoside, as well as such derivatives as 1-(beta-hydroxyethyl)cytosine and 5-(beta-hydroxyethyl)uracil, showing that the enzyme does not have an absolute requirement for a 5-membered sugar ring, but rather for the presence of a primary hydroxyl group. 4. The phosphorylated derivatives of galactopyranosyluracil, and of both hydroxyethyl pyrimidines, were resistant to 5'-nucleotidase. E. coli alkaline phosphatase converted all three nucleotides quantitatively to the starting compounds. 5. A synthesis of 1-(beta-hydroxyethyl)cytosine is described."}
{"id": "PMID:1093346", "title": "Effects of dihydrotestosterone on LH release induced by LH-RH in men.", "content": "In order to determine the effect of dihydrotestosterone on the feed back mechanisim of the hypothalamo-pituitary-testis axis, nine healthy subjects were subjected to LH-RH tests before and after treatment with 5alpha-DHT. A double antibody radio-immunoassay technique was used to measure the concentration of LH and FSH in the plasma. Our results clearly show that the pituitary LH release by LH-RH after pre-treatment with 5alpha-DHT is more prononeced. In the light of actual findings from several laboratories it is not possible to interpret clearly the results of our own experiments, and hence we advance only some hypotheses.", "contents": "Effects of dihydrotestosterone on LH release induced by LH-RH in men. In order to determine the effect of dihydrotestosterone on the feed back mechanisim of the hypothalamo-pituitary-testis axis, nine healthy subjects were subjected to LH-RH tests before and after treatment with 5alpha-DHT. A double antibody radio-immunoassay technique was used to measure the concentration of LH and FSH in the plasma. Our results clearly show that the pituitary LH release by LH-RH after pre-treatment with 5alpha-DHT is more prononeced. In the light of actual findings from several laboratories it is not possible to interpret clearly the results of our own experiments, and hence we advance only some hypotheses."}
{"id": "PMID:1093347", "title": "Accumulation of alprenolol in some polypeptide hormone producing cells and in melanin-containing tissues.", "content": "The distribution of the beta-adrenergic-receptor blocking drug alprenolol labelled with 3H was studied in mice by whole body autoradiography. The most characteristic feature in the distribution pattern was the accumulation in endocrine cells within the pancreatic islets, the adrnal medulla, the pituitary gland and some cells of the thyroid, presumably representing the parafollicular cells. A hgih accumulation was also observed in melanin-containing tissues.", "contents": "Accumulation of alprenolol in some polypeptide hormone producing cells and in melanin-containing tissues. The distribution of the beta-adrenergic-receptor blocking drug alprenolol labelled with 3H was studied in mice by whole body autoradiography. The most characteristic feature in the distribution pattern was the accumulation in endocrine cells within the pancreatic islets, the adrnal medulla, the pituitary gland and some cells of the thyroid, presumably representing the parafollicular cells. A hgih accumulation was also observed in melanin-containing tissues."}
{"id": "PMID:1093348", "title": "Further studies on enzymic inactivation of luteinizing hormone- releasing hormone (LH-RH) by peptidases in the rat hypothalamus.", "content": "Luteinizing hormone-releasing hormone (LH-RH) is known to be inactivated by peptidases in the rat hypothalamus with consequent loss of LH-releasing ability. To make a further study of the peptidases' action on the decapeptide, synthetic LH-RH and its [1-9NH2] analogue were incubated with the supernatant hypothalamic fraction containing the enzyme activity. Using an assay system measuring gonadotrophin release in ovariectomized/steroid-primed rats, both LH-RH and the [1-9NH2] analogue were found to be inactivated to different extents after incubation with the fraction, the analogue completely losing both LH- and FSH-releasing activity, and the releasing hormone almost completely losing its LH- and totally losing its FSH-releasing activity. The findings extend the initial studies by showing that the peptidases can remove both the decapeptide's intrinsic LH- and FSH-releasing activity and that these enzymes act on LH-RH at a site other than the C-terminal glycinamide, since they are able to inactivate the [1-9NH2] analogue lacking this residue.", "contents": "Further studies on enzymic inactivation of luteinizing hormone- releasing hormone (LH-RH) by peptidases in the rat hypothalamus. Luteinizing hormone-releasing hormone (LH-RH) is known to be inactivated by peptidases in the rat hypothalamus with consequent loss of LH-releasing ability. To make a further study of the peptidases' action on the decapeptide, synthetic LH-RH and its [1-9NH2] analogue were incubated with the supernatant hypothalamic fraction containing the enzyme activity. Using an assay system measuring gonadotrophin release in ovariectomized/steroid-primed rats, both LH-RH and the [1-9NH2] analogue were found to be inactivated to different extents after incubation with the fraction, the analogue completely losing both LH- and FSH-releasing activity, and the releasing hormone almost completely losing its LH- and totally losing its FSH-releasing activity. The findings extend the initial studies by showing that the peptidases can remove both the decapeptide's intrinsic LH- and FSH-releasing activity and that these enzymes act on LH-RH at a site other than the C-terminal glycinamide, since they are able to inactivate the [1-9NH2] analogue lacking this residue."}
{"id": "PMID:1093349", "title": "Circadian rhythms of the endocrine pancreas.  A quantitative biochemical and immumocytochemical study.", "content": "Cellular hormone levels of insulin and glucagon in the endocrine pancreas of the rat have been observed and quantified over the 24-hours period, at 3-hourly intervals, by T.V. image conversion analysis on immunocytochemical preparations.  Parallel estimations of blood sugar were carried out.  Glucagon levels were at their lowest when the blood sugar levels were at their highest.  Quantitative cytochemistry is regarded as a valuable tool for the investigation of changes in intracellular hormone levels under physiological and pathological conditions.", "contents": "Circadian rhythms of the endocrine pancreas.  A quantitative biochemical and immumocytochemical study. Cellular hormone levels of insulin and glucagon in the endocrine pancreas of the rat have been observed and quantified over the 24-hours period, at 3-hourly intervals, by T.V. image conversion analysis on immunocytochemical preparations.  Parallel estimations of blood sugar were carried out.  Glucagon levels were at their lowest when the blood sugar levels were at their highest.  Quantitative cytochemistry is regarded as a valuable tool for the investigation of changes in intracellular hormone levels under physiological and pathological conditions."}
{"id": "PMID:1093351", "title": "Level diagnosis of symptomatic urinary tract infections in childhood.", "content": "As no method for localization of urinary tract infection has been shown to be absolutely reliable, six procedures have been carried out simultaneously in 25 girls with acute symptomatic infections and the reliability of each method assessed. While clinical diagnosis of pyelonephritis or cystitis correlated well with the overall results of the battery of tests, the reliability of individual tests varied. Highest reliability was obtained with CRP determinations followed by antibody titration, sedimentation rate, and renal concentrating capacity. In the bladder washout test only 8 of the 14 patients with pyelonephritis had findings clearly indicating high infection. Intermittent or inadequate discharge of bacteria from the renal parenchyma is suggested as the major source for this inaccuracy. In fact, half of the 42 final washout specimens from girls with acute pyelonephritis contained less than 1000 bacteria per ml, indicating that low numbers of organisms in ureteric urine is common in childhood pyelonephritis.", "contents": "Level diagnosis of symptomatic urinary tract infections in childhood. As no method for localization of urinary tract infection has been shown to be absolutely reliable, six procedures have been carried out simultaneously in 25 girls with acute symptomatic infections and the reliability of each method assessed. While clinical diagnosis of pyelonephritis or cystitis correlated well with the overall results of the battery of tests, the reliability of individual tests varied. Highest reliability was obtained with CRP determinations followed by antibody titration, sedimentation rate, and renal concentrating capacity. In the bladder washout test only 8 of the 14 patients with pyelonephritis had findings clearly indicating high infection. Intermittent or inadequate discharge of bacteria from the renal parenchyma is suggested as the major source for this inaccuracy. In fact, half of the 42 final washout specimens from girls with acute pyelonephritis contained less than 1000 bacteria per ml, indicating that low numbers of organisms in ureteric urine is common in childhood pyelonephritis."}
{"id": "PMID:1093352", "title": "Betamethasone 17-valerate in childhood asthma. A double-blind crossover trial in children not taking other corticosteroid therapy.", "content": "A double-blind crossover trial of Betamethasone 17-Valerate steroid aerosol compared with placebo was carried out on 18 children with severe intermittent or chronic asthma. Seventeen of the 18 children showed a clinically obvious improvement on the active drug. The remaining child was unable to use the inhaler properly. In 14 children the response was dramatic. Improvement was shown at home by a reduction in the number of asthmatic symptoms, improvement in twice daily peak flow rates and by a lowered intake of sympathomimetic tablets. At monthly clinic visits, improvement was shown by chest examination, weight gain and by detailed respiratory function tests. By each of these criteria for improvement the benefit of Betamethasone 17-Valerate over the placebo was highly significant. There were no side effects attributable to the therapy and no evidence, as judged by short tetracosactrin tests, of adrenal suppression resulting from the drug. Betamethasone 17-Valerate appears to be a safe and effective therapy in children with severe asthma.", "contents": "Betamethasone 17-valerate in childhood asthma. A double-blind crossover trial in children not taking other corticosteroid therapy. A double-blind crossover trial of Betamethasone 17-Valerate steroid aerosol compared with placebo was carried out on 18 children with severe intermittent or chronic asthma. Seventeen of the 18 children showed a clinically obvious improvement on the active drug. The remaining child was unable to use the inhaler properly. In 14 children the response was dramatic. Improvement was shown at home by a reduction in the number of asthmatic symptoms, improvement in twice daily peak flow rates and by a lowered intake of sympathomimetic tablets. At monthly clinic visits, improvement was shown by chest examination, weight gain and by detailed respiratory function tests. By each of these criteria for improvement the benefit of Betamethasone 17-Valerate over the placebo was highly significant. There were no side effects attributable to the therapy and no evidence, as judged by short tetracosactrin tests, of adrenal suppression resulting from the drug. Betamethasone 17-Valerate appears to be a safe and effective therapy in children with severe asthma."}
{"id": "PMID:1093353", "title": "Escherichia coli serogroups in breast-fed and bottle-fed infants.", "content": "1) Eighty-seven breast-fed and 95 bottle-fed infants were examined for differences in Escherichia coli serogroup pattern. 2) The serogroup composition was less complex in breast-fed infants. Fewer serogroups were found in the individual breast-fed child. However, with few exceptions, the same O groups were found in the two groups of infants. The prevalent O groups were among those also found most frequently in faeces from adults. 06 strains were more common in the breast-fed group, while 07 and 016 were not at all found in that group. 3) A special search for E. coli strains with Kl antigen showed that this antigen predominated in the bottle-fed infants. 4) Klebsiella and other Enterobacteriaceae were more frequently found among bottle-fed infants. 5) It is concluded that the intestinal milieu exerts a selective pressure which may result in a selection of different serotypes in two different intestinal milieus such as those examined.", "contents": "Escherichia coli serogroups in breast-fed and bottle-fed infants. 1) Eighty-seven breast-fed and 95 bottle-fed infants were examined for differences in Escherichia coli serogroup pattern. 2) The serogroup composition was less complex in breast-fed infants. Fewer serogroups were found in the individual breast-fed child. However, with few exceptions, the same O groups were found in the two groups of infants. The prevalent O groups were among those also found most frequently in faeces from adults. 06 strains were more common in the breast-fed group, while 07 and 016 were not at all found in that group. 3) A special search for E. coli strains with Kl antigen showed that this antigen predominated in the bottle-fed infants. 4) Klebsiella and other Enterobacteriaceae were more frequently found among bottle-fed infants. 5) It is concluded that the intestinal milieu exerts a selective pressure which may result in a selection of different serotypes in two different intestinal milieus such as those examined."}
{"id": "PMID:1093354", "title": "The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called \"Kco\", possessed by calf and lamb enteropathogenic strains.", "content": "The transmissible antigen in enteropathogenic E. coli strains from calf and lamb, previously called Kco, is established as the E. coli K99 antigen. It is probably of protein nature since it is destroyed by heating. It is pointed out that other antigens present, growth medium and unknown factors are of great importance for the demonstration of this antigen.", "contents": "The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called \"Kco\", possessed by calf and lamb enteropathogenic strains. The transmissible antigen in enteropathogenic E. coli strains from calf and lamb, previously called Kco, is established as the E. coli K99 antigen. It is probably of protein nature since it is destroyed by heating. It is pointed out that other antigens present, growth medium and unknown factors are of great importance for the demonstration of this antigen."}
{"id": "PMID:1093355", "title": "Influence of methylprednisolone on complement activity in germfree rats and on antibody activity to Escherichia coli in monocontaminated rats.", "content": "By means of radio-isotope technique, the influence of methylprednisolone treatment on opsonic and bactericidal serum activities against E. coli was studied on germfree and monocontaminated rats. Based on the present results it seems unlikely that methylprednisolone impairs the production of complement components. A slight suppression of antibody activity was only found in markedly wasted monocontaminated rats treated with very high doses of methylprednisolone from the day before contamination.", "contents": "Influence of methylprednisolone on complement activity in germfree rats and on antibody activity to Escherichia coli in monocontaminated rats. By means of radio-isotope technique, the influence of methylprednisolone treatment on opsonic and bactericidal serum activities against E. coli was studied on germfree and monocontaminated rats. Based on the present results it seems unlikely that methylprednisolone impairs the production of complement components. A slight suppression of antibody activity was only found in markedly wasted monocontaminated rats treated with very high doses of methylprednisolone from the day before contamination."}
{"id": "PMID:1093356", "title": "Occurrence of K88-negative escherichia coli serotypes in pigs with post weaning diarrhoea.", "content": "Faecal swabs from pigs with post weaning E. coli diarrhoea in 43 different herds were bacteriologically examined. A survey of the isolated haemolytic E. coli strains showed that only 1 of the examined strains possessed the K88 antigens and that E. coli strains belonging to serogroup O149:K91 were most frequently isolated. The results suggest that the K88 antigen is of minor significance in the development of post weaning diarrhoea associated with E. coli serotype O149:K91.", "contents": "Occurrence of K88-negative escherichia coli serotypes in pigs with post weaning diarrhoea. Faecal swabs from pigs with post weaning E. coli diarrhoea in 43 different herds were bacteriologically examined. A survey of the isolated haemolytic E. coli strains showed that only 1 of the examined strains possessed the K88 antigens and that E. coli strains belonging to serogroup O149:K91 were most frequently isolated. The results suggest that the K88 antigen is of minor significance in the development of post weaning diarrhoea associated with E. coli serotype O149:K91."}
{"id": "PMID:1093360", "title": "Comparison of defensive behavior evoked by chemical and electrical stimulation of the hypothalamus in cats.", "content": "Comparison of defensive behavior evoked by chemical anf electrical stimulation of the hypothalamus in cats. Acta Physiol. Pol. 1975, 26 (1) 23--31. An injection of 5 mug of carbachol, as well as electrostimulation of the same hypothalamic area evoked in cats the same type of defensive behavior -- rage reaction. Experiments with carbachol injection and electrostimulation after blockade of the hypothalamic muscarinic receptors (10 mug of atropine) indicate that rage reaction is evoked in a different way by the two methods. Controversial data regarding localization of the hypothalamic \"centres\" responsible for particular type of defensive reactions are discussed.", "contents": "Comparison of defensive behavior evoked by chemical and electrical stimulation of the hypothalamus in cats. Comparison of defensive behavior evoked by chemical anf electrical stimulation of the hypothalamus in cats. Acta Physiol. Pol. 1975, 26 (1) 23--31. An injection of 5 mug of carbachol, as well as electrostimulation of the same hypothalamic area evoked in cats the same type of defensive behavior -- rage reaction. Experiments with carbachol injection and electrostimulation after blockade of the hypothalamic muscarinic receptors (10 mug of atropine) indicate that rage reaction is evoked in a different way by the two methods. Controversial data regarding localization of the hypothalamic \"centres\" responsible for particular type of defensive reactions are discussed."}
{"id": "PMID:1093366", "title": "Breast reconstruction after radical mastectomy.", "content": "The loss of the female breast by mastectomy for treatment of carcinoma is frequently a depressing psychologic experience. While some women learn to cope with the loss, others do not despite the use of specially constructed brassieres. Adaptations of implantation materials and basic plastic surgical techniques are now available for selected postmastectomy patients. These can be useful in modifying the altered body image of the patient who has lost her breast.", "contents": "Breast reconstruction after radical mastectomy. The loss of the female breast by mastectomy for treatment of carcinoma is frequently a depressing psychologic experience. While some women learn to cope with the loss, others do not despite the use of specially constructed brassieres. Adaptations of implantation materials and basic plastic surgical techniques are now available for selected postmastectomy patients. These can be useful in modifying the altered body image of the patient who has lost her breast."}
{"id": "PMID:1093368", "title": "Avulsion injuries in patients receiving corticosteroids.", "content": "Minor trauma often produces avulsion injuries to the skin and soft tissue of patients who are receiving corticosteroid therapy on a continuing basis. Effective emergency management of these wounds reduces the duration of disability for the patient. Early grafting is a more conservative course in terms of predictable patient recovery than watchful waiting. The most important aspects of this treatment are immediate grafting and sufficient immobilization of the graft by the dressing.", "contents": "Avulsion injuries in patients receiving corticosteroids. Minor trauma often produces avulsion injuries to the skin and soft tissue of patients who are receiving corticosteroid therapy on a continuing basis. Effective emergency management of these wounds reduces the duration of disability for the patient. Early grafting is a more conservative course in terms of predictable patient recovery than watchful waiting. The most important aspects of this treatment are immediate grafting and sufficient immobilization of the graft by the dressing."}
{"id": "PMID:1093370", "title": "A new method for automated detection of the R-on-T phenomenon.", "content": "The detection of premature ectopic beats coinciding with the T-wave of the antecedent beat is important for patient monitoring. In the method described here, the difficulties of direct measurement of QT time are avoided by evaluating the changes of QT time according to heart rate by use of a diode function generator. An analog circuit is used for comparing the computed QT time with the coupling interval of successive beats. In case of a R-on-T-phenomenon an alarm is given. The frequency of alarms is registered on a trend recorder. The methods is based on empirical equations which all contain a constant factor. Our investigations showed that the variability of this factor during longer periods of monitoring was small enough to allow sufficient exactness of the evaluation procedure.", "contents": "A new method for automated detection of the R-on-T phenomenon. The detection of premature ectopic beats coinciding with the T-wave of the antecedent beat is important for patient monitoring. In the method described here, the difficulties of direct measurement of QT time are avoided by evaluating the changes of QT time according to heart rate by use of a diode function generator. An analog circuit is used for comparing the computed QT time with the coupling interval of successive beats. In case of a R-on-T-phenomenon an alarm is given. The frequency of alarms is registered on a trend recorder. The methods is based on empirical equations which all contain a constant factor. Our investigations showed that the variability of this factor during longer periods of monitoring was small enough to allow sufficient exactness of the evaluation procedure."}
{"id": "PMID:1093374", "title": "Nonparoxysmal junctional tachycardia in acute myocardial infarction: computer-assisted detection.", "content": "Thirty consecutive patients with acture myocardial infarction had continuous magnetic tape recording of their stay in the coronary care unit. Analysis of the 24 hour tape recordings was implemented on a Honeywell model 316 digital computer. In the first 24 hours after admission to the coronary care unit, 12 of the 30 patients (40 percent) exhibited nonparoxysmal junctional tachycardia; in 5 the arrhythmia was not recognized by conventional monitoring techniques. For the subsequent 3 days, the incidence rate of the arrhythmia was 13 percent for the first 48 hours and 3 percent for 72 hours. Although the mortality rate in patients with nonparoxysmal junctional tachycardia was greater than in patients not demonstrating the arrhythmia (33 versus 6 percent), there was a greater percentage of patients with anterior infarction in the former group; therefore, mortality may have been related to site of infarction rather than being reflective of the arrhythmia or its associated pathophysiologic state. Of possible significance is the association of a greater degree of sinus arrhythmia with nonparoxysmal junctional tachycardia. The incidence of nonparoxysmal junctional tachycardia in this group of patients was greater than previously reported. It is possible that computer tape analysis may have provided more accurate recognition of the arrhythmia and, thus, more realistic incidence data. The association of nonparoxysmal junctional tachycardia with sinus arrhythmia could only have been recognized by computer technique. The computer system is not a diagnostic system but rather a tape review method.", "contents": "Nonparoxysmal junctional tachycardia in acute myocardial infarction: computer-assisted detection. Thirty consecutive patients with acture myocardial infarction had continuous magnetic tape recording of their stay in the coronary care unit. Analysis of the 24 hour tape recordings was implemented on a Honeywell model 316 digital computer. In the first 24 hours after admission to the coronary care unit, 12 of the 30 patients (40 percent) exhibited nonparoxysmal junctional tachycardia; in 5 the arrhythmia was not recognized by conventional monitoring techniques. For the subsequent 3 days, the incidence rate of the arrhythmia was 13 percent for the first 48 hours and 3 percent for 72 hours. Although the mortality rate in patients with nonparoxysmal junctional tachycardia was greater than in patients not demonstrating the arrhythmia (33 versus 6 percent), there was a greater percentage of patients with anterior infarction in the former group; therefore, mortality may have been related to site of infarction rather than being reflective of the arrhythmia or its associated pathophysiologic state. Of possible significance is the association of a greater degree of sinus arrhythmia with nonparoxysmal junctional tachycardia. The incidence of nonparoxysmal junctional tachycardia in this group of patients was greater than previously reported. It is possible that computer tape analysis may have provided more accurate recognition of the arrhythmia and, thus, more realistic incidence data. The association of nonparoxysmal junctional tachycardia with sinus arrhythmia could only have been recognized by computer technique. The computer system is not a diagnostic system but rather a tape review method."}
{"id": "PMID:1093375", "title": "Diagnosis and management of complications of prosthetic heart valves.", "content": "Complications after heart valve replacement remain a substantial source of morbidity and mortality despite continuing advances in surgical care and prosthetic design. Infectious endocarditis occurs in about 4 percent of patients and may appear early (within 60 days) or late after operation. Endocarditis of early onset is commonly due to staphylococcal, fungal or gram-negative organisms and is fatal in 70 percent or more of cases. Infection of late onset is more often of streptococcal origin and the mortality rate is lower, about 35 percent. With either type, prompt recognition, vigorous and appropriate antimicrobial therapy and early consideration of surgical intervention are crucial. The postperfusion and postpericardiotomy syndromes are relatively common and relatively benign syndromes associated with postoperative fever. Their recognition is important to prevent confusion with endocarditis or sepsis and thus to reassure the patient and physician. Treatment is primarily symptomatic. Intravascular hemolysis occurs with most prosthetic heart valves but is more common with certain prostheses and with paraprosthetic valve regurgitation, with significant hemolytic anemia in 5 to 15 percent. Oral iron replacement therapy is effective in the majority of patients, but occasionally blood transfusion or reoperation for leak around the prosthesis is necessary. Prosthesis dysfunction due to thrombus may be recognized clinically by recurrence of heart failure, syncope, cardiomegaly and altered prosthetic valve sounds or new murmurs. Hemodynamic studies verify the diagnosis, and prompt reoperation is indicated for this potentially lethal problem. Systemic embolization has decreased markedly with the introduction of cloth-covered prostheses and is frequently related to erratic or ineffective anticoagulant therapy. We continue to recommend anticoagulant therapy for all patients with prosthetic heart valves unless there is a major contraindication.", "contents": "Diagnosis and management of complications of prosthetic heart valves. Complications after heart valve replacement remain a substantial source of morbidity and mortality despite continuing advances in surgical care and prosthetic design. Infectious endocarditis occurs in about 4 percent of patients and may appear early (within 60 days) or late after operation. Endocarditis of early onset is commonly due to staphylococcal, fungal or gram-negative organisms and is fatal in 70 percent or more of cases. Infection of late onset is more often of streptococcal origin and the mortality rate is lower, about 35 percent. With either type, prompt recognition, vigorous and appropriate antimicrobial therapy and early consideration of surgical intervention are crucial. The postperfusion and postpericardiotomy syndromes are relatively common and relatively benign syndromes associated with postoperative fever. Their recognition is important to prevent confusion with endocarditis or sepsis and thus to reassure the patient and physician. Treatment is primarily symptomatic. Intravascular hemolysis occurs with most prosthetic heart valves but is more common with certain prostheses and with paraprosthetic valve regurgitation, with significant hemolytic anemia in 5 to 15 percent. Oral iron replacement therapy is effective in the majority of patients, but occasionally blood transfusion or reoperation for leak around the prosthesis is necessary. Prosthesis dysfunction due to thrombus may be recognized clinically by recurrence of heart failure, syncope, cardiomegaly and altered prosthetic valve sounds or new murmurs. Hemodynamic studies verify the diagnosis, and prompt reoperation is indicated for this potentially lethal problem. Systemic embolization has decreased markedly with the introduction of cloth-covered prostheses and is frequently related to erratic or ineffective anticoagulant therapy. We continue to recommend anticoagulant therapy for all patients with prosthetic heart valves unless there is a major contraindication."}
{"id": "PMID:1093376", "title": "Renal consequences of valvular heart disease.", "content": "Three aspects of potential renal involvement in patients with valvular heart disease are discussed. These include (1) disturbances in renal salt and water handling and their implications with respect to diuretic management; (2) the hemodynamic effects of angiographic contrast agents with identification of potentially dangerous effects on the kidneys; and (3) the histologic patterns of bacterial endocarditis in the kidney and their similarity to those of immunologically mediated disease.", "contents": "Renal consequences of valvular heart disease. Three aspects of potential renal involvement in patients with valvular heart disease are discussed. These include (1) disturbances in renal salt and water handling and their implications with respect to diuretic management; (2) the hemodynamic effects of angiographic contrast agents with identification of potentially dangerous effects on the kidneys; and (3) the histologic patterns of bacterial endocarditis in the kidney and their similarity to those of immunologically mediated disease."}
{"id": "PMID:1093379", "title": "Oral contraceptives and ascorbic acid.", "content": "Plasma, leukocyte, and platelet ascorbic acid levels are decreased in women ingesting oral contraceptive steroids. Studies have shown that it is the estrogenic component of the oral contraceptive agents that is associated with the decresased ascorbic acid concentrations. Urinary excretion of ascorbic acid does not appear to be increased by the steroids. Although serum levels of copper are increased by estrogens and oral contraceptives, ascorbic acid catabolism does not appear to be increased (unpublished). Our preliminary data on tissue uptake of ascorbic acid suggest that changes in tissue distribution are one possible answer for the observed effects of the steroids on blood levels of ascorbic acid.", "contents": "Oral contraceptives and ascorbic acid. Plasma, leukocyte, and platelet ascorbic acid levels are decreased in women ingesting oral contraceptive steroids. Studies have shown that it is the estrogenic component of the oral contraceptive agents that is associated with the decresased ascorbic acid concentrations. Urinary excretion of ascorbic acid does not appear to be increased by the steroids. Although serum levels of copper are increased by estrogens and oral contraceptives, ascorbic acid catabolism does not appear to be increased (unpublished). Our preliminary data on tissue uptake of ascorbic acid suggest that changes in tissue distribution are one possible answer for the observed effects of the steroids on blood levels of ascorbic acid."}
{"id": "PMID:1093382", "title": "Control of brain monoamine synthesis by diet and plasma amino acids.", "content": "The rates at which monoaminergic neurons in rat brains synthesize their neurotransmitters depend on the availability of the amino acid precursors tryptophan (for serotonin) and tyrosine (for dopamine and norepinephrine). The administration of tryptophan, the injection of insulin, or the consumption of a single protein-free high-carbohydrate meal all elevate brain tryptophan levels and, soon thereafter, the levels of serotonin and its major metabolite 5-hydroxyindole acetic acid. The addition of protein to the meal suppresses the increases in brain tryptophan and serotonin, because protein contributes to plasma considerably larger amounts of the other neutral amino acids (e.g., leucine, phenylalanine) than of tryptophan, and these other amino acids compete with tryptophan for uptake into the brain. The elevation of brain tyrosine (by injection of the amino acid or consumption of a single 40% protein meal) accelerates brain catecholamine synthesis, as estimated by measuring brain dopa accumulation after decarboxylase inhibition, or brain catecholamine accumulation after inhibition of monoamine oxidase. These observations suggest that serotonin- and catecholamine-containing brain neurons are normally under specific dietary control.", "contents": "Control of brain monoamine synthesis by diet and plasma amino acids. The rates at which monoaminergic neurons in rat brains synthesize their neurotransmitters depend on the availability of the amino acid precursors tryptophan (for serotonin) and tyrosine (for dopamine and norepinephrine). The administration of tryptophan, the injection of insulin, or the consumption of a single protein-free high-carbohydrate meal all elevate brain tryptophan levels and, soon thereafter, the levels of serotonin and its major metabolite 5-hydroxyindole acetic acid. The addition of protein to the meal suppresses the increases in brain tryptophan and serotonin, because protein contributes to plasma considerably larger amounts of the other neutral amino acids (e.g., leucine, phenylalanine) than of tryptophan, and these other amino acids compete with tryptophan for uptake into the brain. The elevation of brain tyrosine (by injection of the amino acid or consumption of a single 40% protein meal) accelerates brain catecholamine synthesis, as estimated by measuring brain dopa accumulation after decarboxylase inhibition, or brain catecholamine accumulation after inhibition of monoamine oxidase. These observations suggest that serotonin- and catecholamine-containing brain neurons are normally under specific dietary control."}
{"id": "PMID:1093386", "title": "Lymphocyte surface receptors and leukemia virus-induced immunosuppression.", "content": "The number and distribution patterns of lymphocytes in the spleens and lymph nodes of Balb/c mice which express immunoglobulin surface receptors were studied in terms of the effects of a murine leukemia virus on the immune-response mechanism. Friend leukemia virus induces a prompt, marked depression of the immune response of mice to antigens such as sheep erythrocytes and E. coli LPS. A functioning T- and B-lymphocyte system is necessary for the response to the SRBC's whereas E. coli LPS, a T cell-independent antigen, stimulates B cells alone. Although the responses to both classes of antigen were markedly depressed in FLV-infected mice, the major defect appeared to be impairment of B-cell function, at least early in the course of infection. In order to examine in more detail the mechanism of interaction between FLV and lymphoid cells with Ig surface receptors, presumably B cells, immmunofluorescent analyses were performed with spleen, and lymph node cells from FLV-infected mice. Within a few days after infection there was a marked decrease in the percentage of spleen cells with Ig surface molecules, although the absolute number of these cells was either unchanged or increased due to marked splenomegaly caused by the virus. A marked decrease in the percentage of splenocytes with theta antigen, considered a marker for mature T cells, also was evident in infected mice. The number of spleen cells showing evidence of FLV infection (i.e., positive for FLV-associated antigens) increased rapidly during the first few days after infection, and within 2 to 2 1/2 weeks nearly all of the nucleated splenocytes were positive for the tumor antigen. In contrast to the results for spleen cells, there were increases rather than decreases in the percentages of Ig-positive and theta-positive cells in the lymph nodes after infection. The number of lymph-node cells that showed the presence of FLV antigen was much lower than in the spleen, and their appearance was also much slower as the leukemic process progressed. Despite these differences between spleen and lymph-node cells in terms of relative percentages of Ig- and theta-positive lymphocytes, relatively similar depressions were evident for the percentages of lymphoid cells that could redistribute their surface Ig receptors into polar caps when incubated with anti-Ig serum at 37 C. Marked impairment of the Ig-capping responses for both spleen and lymph-node cells paralleled the course of infection and development of immunosuppression. These observations indicate that murine leukemia virus infection can both alter the responsiveness of immunocompetent cells to T-dependent and independent antigens and depress the number and normal functional activity of these cells, as reflected by altered surface Ig receptors and antigens.", "contents": "Lymphocyte surface receptors and leukemia virus-induced immunosuppression. The number and distribution patterns of lymphocytes in the spleens and lymph nodes of Balb/c mice which express immunoglobulin surface receptors were studied in terms of the effects of a murine leukemia virus on the immune-response mechanism. Friend leukemia virus induces a prompt, marked depression of the immune response of mice to antigens such as sheep erythrocytes and E. coli LPS. A functioning T- and B-lymphocyte system is necessary for the response to the SRBC's whereas E. coli LPS, a T cell-independent antigen, stimulates B cells alone. Although the responses to both classes of antigen were markedly depressed in FLV-infected mice, the major defect appeared to be impairment of B-cell function, at least early in the course of infection. In order to examine in more detail the mechanism of interaction between FLV and lymphoid cells with Ig surface receptors, presumably B cells, immmunofluorescent analyses were performed with spleen, and lymph node cells from FLV-infected mice. Within a few days after infection there was a marked decrease in the percentage of spleen cells with Ig surface molecules, although the absolute number of these cells was either unchanged or increased due to marked splenomegaly caused by the virus. A marked decrease in the percentage of splenocytes with theta antigen, considered a marker for mature T cells, also was evident in infected mice. The number of spleen cells showing evidence of FLV infection (i.e., positive for FLV-associated antigens) increased rapidly during the first few days after infection, and within 2 to 2 1/2 weeks nearly all of the nucleated splenocytes were positive for the tumor antigen. In contrast to the results for spleen cells, there were increases rather than decreases in the percentages of Ig-positive and theta-positive cells in the lymph nodes after infection. The number of lymph-node cells that showed the presence of FLV antigen was much lower than in the spleen, and their appearance was also much slower as the leukemic process progressed. Despite these differences between spleen and lymph-node cells in terms of relative percentages of Ig- and theta-positive lymphocytes, relatively similar depressions were evident for the percentages of lymphoid cells that could redistribute their surface Ig receptors into polar caps when incubated with anti-Ig serum at 37 C. Marked impairment of the Ig-capping responses for both spleen and lymph-node cells paralleled the course of infection and development of immunosuppression. These observations indicate that murine leukemia virus infection can both alter the responsiveness of immunocompetent cells to T-dependent and independent antigens and depress the number and normal functional activity of these cells, as reflected by altered surface Ig receptors and antigens."}
{"id": "PMID:1093387", "title": "The effects of consultation. A review of empirical studies.", "content": "Although consultation, as an activity for intervention, has achieved considerable popularity among human service professionals and figures prominently in current federal mental health legislation, a basic question still unanswerable is, \"Does it work?\" This paper reviews 35 consultation outcome studies reported between 1958 and 1972 in an attempt to provide some answers to this question. Although their quality varies greatly, the evidence seems to lend some support to the effectiveness of consultation as a change method. In 69% of the studies reviewed, positive change was demonstrated on the consultee, client, or system level or at some combination of these. The review also suggests that, despite a need for studies which build cumulatively on earlier ones so as to provide comparable data instead of a conglomerate of isolated findings, progress has been made in the evaluation of consultation.", "contents": "The effects of consultation. A review of empirical studies. Although consultation, as an activity for intervention, has achieved considerable popularity among human service professionals and figures prominently in current federal mental health legislation, a basic question still unanswerable is, \"Does it work?\" This paper reviews 35 consultation outcome studies reported between 1958 and 1972 in an attempt to provide some answers to this question. Although their quality varies greatly, the evidence seems to lend some support to the effectiveness of consultation as a change method. In 69% of the studies reviewed, positive change was demonstrated on the consultee, client, or system level or at some combination of these. The review also suggests that, despite a need for studies which build cumulatively on earlier ones so as to provide comparable data instead of a conglomerate of isolated findings, progress has been made in the evaluation of consultation."}
{"id": "PMID:1093388", "title": "Early secondary prevention with disadvantaged preschool children.", "content": "A body of research data is reviewed pointing to considerable intellectual lag and nonstandard linguistic abilities among the disadvantaged. While \"difference\" and \"deficit\" theorists interpret these findings differently, many observers agree that early educational intervention may be a useful approach to such problems. A series of early intervention programs is reviewed for three young disadvantaged groups: infants, toddlers, and preschoolers. Findings from these programs suggest that early intervention helps materially to reduce the intellectual and linguistic problems of the young disadvantaged.", "contents": "Early secondary prevention with disadvantaged preschool children. A body of research data is reviewed pointing to considerable intellectual lag and nonstandard linguistic abilities among the disadvantaged. While \"difference\" and \"deficit\" theorists interpret these findings differently, many observers agree that early educational intervention may be a useful approach to such problems. A series of early intervention programs is reviewed for three young disadvantaged groups: infants, toddlers, and preschoolers. Findings from these programs suggest that early intervention helps materially to reduce the intellectual and linguistic problems of the young disadvantaged."}
{"id": "PMID:1093389", "title": "Immunization of children with attenuated measles-rubella bivalent vaccine.", "content": "The effects of simultaneous administration of Schwarz-strain measles virus vaccine and Cendehill-strain rubella virus vaccine as a single bivalent injection was evaluated in 346 children susceptible to both diseases. With placebo and monovalent controls, four bivalent preparations were used in a double-blind protocol to determine the effects of variations in the concentration of one or both components. No unusual reactions occurred and simultaneous administration did not enhance clinical reactivity. For measles, the seroconversion rate was 97% for monovalent vaccine and 97% to 100% (average 98%) for bivalent vaccines. For rubella, the rate was 100% for monovalent vaccine and 94% to 100% (average 99%) for bivalent vaccines. Thus, the vaccines were as safe and effective when given together as when given separately.", "contents": "Immunization of children with attenuated measles-rubella bivalent vaccine. The effects of simultaneous administration of Schwarz-strain measles virus vaccine and Cendehill-strain rubella virus vaccine as a single bivalent injection was evaluated in 346 children susceptible to both diseases. With placebo and monovalent controls, four bivalent preparations were used in a double-blind protocol to determine the effects of variations in the concentration of one or both components. No unusual reactions occurred and simultaneous administration did not enhance clinical reactivity. For measles, the seroconversion rate was 97% for monovalent vaccine and 97% to 100% (average 98%) for bivalent vaccines. For rubella, the rate was 100% for monovalent vaccine and 94% to 100% (average 99%) for bivalent vaccines. Thus, the vaccines were as safe and effective when given together as when given separately."}
{"id": "PMID:1093390", "title": "Autoimmune phenomena in alcholic cirrhosis.", "content": "Serum immunoglobulins usually are increased impressively in alcoholic cirrhosis, particularly IgA and IgM. The basis for these changes has not been clarified. Previous studies with sera of cirrhotic subjects failed to show autoantibody reactions found in other hepatocellular diseases. Two autoantibody reactions were demonstrated by immunofluorescent techniques, using IgG, IgM, and IgA of cirrhotic sera. Purification was achieved by starch block electrophoresis, gel filtration of Sephadex G-200, chromatography on DEAE-Sephadex, and affinity chromatography on columns of Sepharose 4B, in that sequence. IgM appeared as a nonspecific antinuclear antibody, IgA bound to alcoholic hyaline (Mallory bodies). IgG failed to stain any of the examined tissues. It may be the blocking agent which prevents the above-mentioned autoimmune phenomena in whole serum.", "contents": "Autoimmune phenomena in alcholic cirrhosis. Serum immunoglobulins usually are increased impressively in alcoholic cirrhosis, particularly IgA and IgM. The basis for these changes has not been clarified. Previous studies with sera of cirrhotic subjects failed to show autoantibody reactions found in other hepatocellular diseases. Two autoantibody reactions were demonstrated by immunofluorescent techniques, using IgG, IgM, and IgA of cirrhotic sera. Purification was achieved by starch block electrophoresis, gel filtration of Sephadex G-200, chromatography on DEAE-Sephadex, and affinity chromatography on columns of Sepharose 4B, in that sequence. IgM appeared as a nonspecific antinuclear antibody, IgA bound to alcoholic hyaline (Mallory bodies). IgG failed to stain any of the examined tissues. It may be the blocking agent which prevents the above-mentioned autoimmune phenomena in whole serum."}
{"id": "PMID:1093391", "title": "Computer-aided diagnosis of \"dyspepsia\".", "content": "Experience with computer-aided diagnosis of \"dyspepsia\" in a consecutive prospective series of 212 patients coming to surgery is described. Analysis is concentrated upon 122 patients who presented to an outpatient clinic de novo for diagnosis. During their first (outpatient) hospital contact, a firm diagnosis was made in just over half of these patients (though where made, it was usually correct). After full investigation, the diagnostic accuracy (prior to operation) was 92.6%. Using data elicited solely from the house surgeon's interview at the time of admission, the computer's overall diagnostic accuracy was 87.7%. The cost of each new computer diagnosis was around 25 new pence ($0.60). and the time taken was about 5 minutes. In a further small series designed to discriminate between organic and functional dyspepsia, the computer correctly assigned all but 1 of 23 patients with organic disease to the correct disease category. However, almost half of 33 patients with x-ray negative dyspepsia were predicted by the computer to have organic lesions. Time alone will tell whether the computer is a better early predictor of eventual organic disease than currently available radiologic methods.", "contents": "Computer-aided diagnosis of \"dyspepsia\". Experience with computer-aided diagnosis of \"dyspepsia\" in a consecutive prospective series of 212 patients coming to surgery is described. Analysis is concentrated upon 122 patients who presented to an outpatient clinic de novo for diagnosis. During their first (outpatient) hospital contact, a firm diagnosis was made in just over half of these patients (though where made, it was usually correct). After full investigation, the diagnostic accuracy (prior to operation) was 92.6%. Using data elicited solely from the house surgeon's interview at the time of admission, the computer's overall diagnostic accuracy was 87.7%. The cost of each new computer diagnosis was around 25 new pence ($0.60). and the time taken was about 5 minutes. In a further small series designed to discriminate between organic and functional dyspepsia, the computer correctly assigned all but 1 of 23 patients with organic disease to the correct disease category. However, almost half of 33 patients with x-ray negative dyspepsia were predicted by the computer to have organic lesions. Time alone will tell whether the computer is a better early predictor of eventual organic disease than currently available radiologic methods."}
{"id": "PMID:1093392", "title": "The role of fiberoptic endoscopy in the diagnosis and management of duodenal neoplasms.", "content": "This is a study of 11 cases of duodenal tumors encountered in 1200 consecutive duodenoscopic examinations over a 34-month period at The Johns Hopkins Hospital. Of these tumors, 4 were benign and 7 malignant. Endoscopic removal of 2 benign tumors was accomplished without morbidity and obviated the necessity for transabdominal surgery. The 2 other benign tumors were a probable lipoma and a benign polyp, and did not require surgical intervention. In 6 of the 7 patients with malignant lesions, the indication for performing duodenoscopy was an abnormal roentgenographic study. In each case, direct visualization of the abnormal area, together with biopsy and cytologic brushing, provided a definitive diagnosis of malignant disease and helped plan the therapeutic course. In the seventh patient, a primary adenocarcinoma at the duodenojejunal junction was found on endoscopic examination, after all other available diagnostic studies to find the cause of persistent gastrointestinal bleeding had been exhausted. Although duodenal tumors are not common, they were encountered in 1 of every 100 duodenoscopies at a referral center. With increasing use of fiberoptic endoscopy, such tumors will undoubtedly be seen with increasing frequency. In our experience, endoscopy has proved to be a safe and reliable method of distinguishing between benign and malignant duodenal neoplasms and of assistance in planning subsequent management.", "contents": "The role of fiberoptic endoscopy in the diagnosis and management of duodenal neoplasms. This is a study of 11 cases of duodenal tumors encountered in 1200 consecutive duodenoscopic examinations over a 34-month period at The Johns Hopkins Hospital. Of these tumors, 4 were benign and 7 malignant. Endoscopic removal of 2 benign tumors was accomplished without morbidity and obviated the necessity for transabdominal surgery. The 2 other benign tumors were a probable lipoma and a benign polyp, and did not require surgical intervention. In 6 of the 7 patients with malignant lesions, the indication for performing duodenoscopy was an abnormal roentgenographic study. In each case, direct visualization of the abnormal area, together with biopsy and cytologic brushing, provided a definitive diagnosis of malignant disease and helped plan the therapeutic course. In the seventh patient, a primary adenocarcinoma at the duodenojejunal junction was found on endoscopic examination, after all other available diagnostic studies to find the cause of persistent gastrointestinal bleeding had been exhausted. Although duodenal tumors are not common, they were encountered in 1 of every 100 duodenoscopies at a referral center. With increasing use of fiberoptic endoscopy, such tumors will undoubtedly be seen with increasing frequency. In our experience, endoscopy has proved to be a safe and reliable method of distinguishing between benign and malignant duodenal neoplasms and of assistance in planning subsequent management."}
{"id": "PMID:1093393", "title": "Thymus-derived lymphocytes in type B acute viral hepatitis and healthy carriers of hepatitis B surface antigen (HBsAg).", "content": "To assess thymus-derived (T) lymphocyte function in hepatitis B infection, lymphocytes from patients with type B acute viral hepatitis and healthy carriers of the hepatitis B surface antigen, were compared to normal controlss using phytohemagglutinin (PHA) -induced blast cell transformation and sheep-cell rosette formation. Overall cell (T lymphocyte) -mediated immune function was similar with these in vitro assays in all three groups, except for decreases in total T cells in isolated patients with acute disease. Consequently, if a defect in cellular immunity exists in normal controls with persistent hepatitis B infection, it is probably specific for the antigens of the hepatitis B virus and not related to a generalized impairment of T-lymphocyte function.", "contents": "Thymus-derived lymphocytes in type B acute viral hepatitis and healthy carriers of hepatitis B surface antigen (HBsAg). To assess thymus-derived (T) lymphocyte function in hepatitis B infection, lymphocytes from patients with type B acute viral hepatitis and healthy carriers of the hepatitis B surface antigen, were compared to normal controlss using phytohemagglutinin (PHA) -induced blast cell transformation and sheep-cell rosette formation. Overall cell (T lymphocyte) -mediated immune function was similar with these in vitro assays in all three groups, except for decreases in total T cells in isolated patients with acute disease. Consequently, if a defect in cellular immunity exists in normal controls with persistent hepatitis B infection, it is probably specific for the antigens of the hepatitis B virus and not related to a generalized impairment of T-lymphocyte function."}
{"id": "PMID:1093394", "title": "Syphilitic gastropathy.", "content": "A case study of a 22-year-old male with periumbilical, postprandial pain, partially relieved by antacids is presented. Abnormalities demonstrated on upper-gastrointestinal series and a positive FTA-ABS suggested gastric lues. This diagnosis was confirmed by demonstrating spirochetes in gastric mucosa, both by silver impregnation and fluorescent techniques specific for Treponema pallidum.", "contents": "Syphilitic gastropathy. A case study of a 22-year-old male with periumbilical, postprandial pain, partially relieved by antacids is presented. Abnormalities demonstrated on upper-gastrointestinal series and a positive FTA-ABS suggested gastric lues. This diagnosis was confirmed by demonstrating spirochetes in gastric mucosa, both by silver impregnation and fluorescent techniques specific for Treponema pallidum."}
{"id": "PMID:1093398", "title": "Particulate matter in reconstituted amphotericin B and assay of filtered solutions of amphotericin B.", "content": "Amphotericin B colloidal solutions were prepared according to the manufacturer's directions and filtered with membrane filters of different porosity to test for particulate matter; and the concentration and in vitro microbiological activity of filtered and nonfiltered solutions were determined.  The amphotericin B solutions contained numerous particles.  Filtration through 0.85- and 0.45-mum filters did not reduce the in vitro antimicrobial activity, and filtration through 5-, 0.45-, and 0.22-mum filters did not alter the concentration of the drug when assayed spectrophotometrically.", "contents": "Particulate matter in reconstituted amphotericin B and assay of filtered solutions of amphotericin B. Amphotericin B colloidal solutions were prepared according to the manufacturer's directions and filtered with membrane filters of different porosity to test for particulate matter; and the concentration and in vitro microbiological activity of filtered and nonfiltered solutions were determined.  The amphotericin B solutions contained numerous particles.  Filtration through 0.85- and 0.45-mum filters did not reduce the in vitro antimicrobial activity, and filtration through 5-, 0.45-, and 0.22-mum filters did not alter the concentration of the drug when assayed spectrophotometrically."}
{"id": "PMID:1093399", "title": "Economic justification of pharmacist involement in patient medication consultation.", "content": "The financial savings experienced by patients as a result of pharmacists' efforts in a discharge medication interview program were evaluated.  The study involved 1,000 patients discharged from the surgery service of a university hospital.  Pharmacists worked with physicians and patients in reducing the cost of medications by: (1) substituting less expensive drugs when possible, (2) relabeling bedside medication, (3) securing third party coverage for eligible patients, and (4) recommending purchase of non-prescription drugs over the counter.  Savings of nearly +1,700 were realized on 517 out of 1,832 prescriptions; this was equal to +9.13 of savings to the patient per hour of pharmacist consultation time.", "contents": "Economic justification of pharmacist involement in patient medication consultation. The financial savings experienced by patients as a result of pharmacists' efforts in a discharge medication interview program were evaluated.  The study involved 1,000 patients discharged from the surgery service of a university hospital.  Pharmacists worked with physicians and patients in reducing the cost of medications by: (1) substituting less expensive drugs when possible, (2) relabeling bedside medication, (3) securing third party coverage for eligible patients, and (4) recommending purchase of non-prescription drugs over the counter.  Savings of nearly +1,700 were realized on 517 out of 1,832 prescriptions; this was equal to +9.13 of savings to the patient per hour of pharmacist consultation time."}
{"id": "PMID:1093401", "title": "Studies of human lymphocytes in the newborn and the aged.", "content": "Proportions and absolute numbers of T and B lymphocytes were determined among 30 newborn infants and group of 77 elderly patients 60 to 95 years of age. Total lymphocytes in the cord blood of the newborn showed a distinct elevation in total numbers of T and B lymphocytes (p less than 0.005) as compared to that in blood from normal adult controls, reflecting the relative lymphocytosis of infancy. Proportions of cord blood T lymphocytes as reflected by the sheep cell rosette technic were considerable lower than those in lymphocytes from normal adult controls, however, proportions of cord blood T lymphocytes as determined by indirect immunofluorescence were not significantly different from those in controls. Old people showed a significant reduction in total numbers of lymphocytes (p less than 0.005) when compared with those in normal adult controls 18 to 51 years of age. Moreover, there was a significant increase in the relative proportions of peripheral blood B lymphocytes in the elderly although the absolute numbers of B lymphocytes in the elderly although the absolute numbers of B cells did not differ from those in younger controls. A significant decrease in total numbers of T cells as measured both by sheep cell rosettes and indirect immunofluorescence was recorded among older patients (p less than 0.001). In addition, there was a broad increment in the incidence of various autoantibodies (anti-nuclear, andi-IgG, antismooth muscle, antimitochondrial and antiparietal cell) among the old people studies. No direct correlation could be determined between relative B-cell percentage increase or T-cell decrease and the presence of various autoantibodies in individual patients. Diminution in total lymphocyte counts as well as absolute numbers of T cells in the elderly may provide the cellular basis for an increased susceptibility to neoplasia and infection.", "contents": "Studies of human lymphocytes in the newborn and the aged. Proportions and absolute numbers of T and B lymphocytes were determined among 30 newborn infants and group of 77 elderly patients 60 to 95 years of age. Total lymphocytes in the cord blood of the newborn showed a distinct elevation in total numbers of T and B lymphocytes (p less than 0.005) as compared to that in blood from normal adult controls, reflecting the relative lymphocytosis of infancy. Proportions of cord blood T lymphocytes as reflected by the sheep cell rosette technic were considerable lower than those in lymphocytes from normal adult controls, however, proportions of cord blood T lymphocytes as determined by indirect immunofluorescence were not significantly different from those in controls. Old people showed a significant reduction in total numbers of lymphocytes (p less than 0.005) when compared with those in normal adult controls 18 to 51 years of age. Moreover, there was a significant increase in the relative proportions of peripheral blood B lymphocytes in the elderly although the absolute numbers of B lymphocytes in the elderly although the absolute numbers of B cells did not differ from those in younger controls. A significant decrease in total numbers of T cells as measured both by sheep cell rosettes and indirect immunofluorescence was recorded among older patients (p less than 0.001). In addition, there was a broad increment in the incidence of various autoantibodies (anti-nuclear, andi-IgG, antismooth muscle, antimitochondrial and antiparietal cell) among the old people studies. No direct correlation could be determined between relative B-cell percentage increase or T-cell decrease and the presence of various autoantibodies in individual patients. Diminution in total lymphocyte counts as well as absolute numbers of T cells in the elderly may provide the cellular basis for an increased susceptibility to neoplasia and infection."}
{"id": "PMID:1093402", "title": "Gonadal dysfunction in uremic men. A study of the hypothalamo-pituitary-testicular axis before and after renal transplantation.", "content": "Evaluation of testicular function in 13 hemodialyzed patients revealed the following: plasma testosterone (ng/100 ml) was low (less than 300 ng/100 ml) in 6 and low normal in 7 patients; sperm counts ranged from 0 to 8 million/ml and motility from 0 to 8 per cent; testicular tissue from 2 patients showed an abnormal histologic picture ranging from hypospermatogenesis to germinal cell aplasia. Follicle-stimulating hormone (FSH, ng/ml) was normal in eight (10 to 217 ng/ml), and persistently eveated in five patients (265 to 760 ng/ml). Of the latter five patients, two were azzoospermic, one had germinal cell aplasia on postmortem examination, one had virtually no viable sperms, and the other was never able to furnish ejaculate for examination. Luteinizing hormone (LH, mg/ml) was high (more than 210 ng/ml) in five and normal in eight patients. Six patients when given clomiphene showed the normal response of increased FSH and LH release. Four of the 13 patients, when restudied 6 to 12 months later and while still on dialysis, showed further deterioration of plasma testosterone and sperm counts. Four of the patients subsequently underwent successful renal transplantation. All showed improvement in sperm counts (20 to 40 million/ml, motility 40 to 90 per cent) and plasma testosterone (440 to 850 ng/100 ml). These data suggest that both germinal cell and leydig cell functions were impaired among uremic men. These dysfunctions were not correctable by hemodialysis, but were completely reversed by renal transplantation. The high FSH among patients with azzospermia indicates a responsive pituitary. The positive response to clomiphene suggests that storage as well as release of both hypothalamic and pituitary hormones were normal. Attempts to localize a single defect at the testis failed to explain the post-transplant surge of FSH which invariably proceded improvement in spermatogenesis. It is therefore postulated that a defect in that portion of the hypothalamus involved in the receipt and/or interpretation of message might be at fault in uremia.", "contents": "Gonadal dysfunction in uremic men. A study of the hypothalamo-pituitary-testicular axis before and after renal transplantation. Evaluation of testicular function in 13 hemodialyzed patients revealed the following: plasma testosterone (ng/100 ml) was low (less than 300 ng/100 ml) in 6 and low normal in 7 patients; sperm counts ranged from 0 to 8 million/ml and motility from 0 to 8 per cent; testicular tissue from 2 patients showed an abnormal histologic picture ranging from hypospermatogenesis to germinal cell aplasia. Follicle-stimulating hormone (FSH, ng/ml) was normal in eight (10 to 217 ng/ml), and persistently eveated in five patients (265 to 760 ng/ml). Of the latter five patients, two were azzoospermic, one had germinal cell aplasia on postmortem examination, one had virtually no viable sperms, and the other was never able to furnish ejaculate for examination. Luteinizing hormone (LH, mg/ml) was high (more than 210 ng/ml) in five and normal in eight patients. Six patients when given clomiphene showed the normal response of increased FSH and LH release. Four of the 13 patients, when restudied 6 to 12 months later and while still on dialysis, showed further deterioration of plasma testosterone and sperm counts. Four of the patients subsequently underwent successful renal transplantation. All showed improvement in sperm counts (20 to 40 million/ml, motility 40 to 90 per cent) and plasma testosterone (440 to 850 ng/100 ml). These data suggest that both germinal cell and leydig cell functions were impaired among uremic men. These dysfunctions were not correctable by hemodialysis, but were completely reversed by renal transplantation. The high FSH among patients with azzospermia indicates a responsive pituitary. The positive response to clomiphene suggests that storage as well as release of both hypothalamic and pituitary hormones were normal. Attempts to localize a single defect at the testis failed to explain the post-transplant surge of FSH which invariably proceded improvement in spermatogenesis. It is therefore postulated that a defect in that portion of the hypothalamus involved in the receipt and/or interpretation of message might be at fault in uremia."}
{"id": "PMID:1093403", "title": "Infected subfural hematoma: three case reports involving gram-negative organisms.", "content": "Three case reports of infected subdural hematoma are presented, two with Salmonellae and one with Escherichia. Infection of such hematomas most often occurs during bacteremia, and the area of infection is limited by the existing hematoma membrane. Clincally, fever, headache, nuchal rigidity, and focal neurological signs, especially in a patient with previous head trauma, suggest subdural infection. Treatment consists of drainage and systemic antibiotics. These case reports demonstrate the clinical features of this rarely reported entity, and especially illustrate the need for careful bacteriologic identification in suspected cases.", "contents": "Infected subfural hematoma: three case reports involving gram-negative organisms. Three case reports of infected subdural hematoma are presented, two with Salmonellae and one with Escherichia. Infection of such hematomas most often occurs during bacteremia, and the area of infection is limited by the existing hematoma membrane. Clincally, fever, headache, nuchal rigidity, and focal neurological signs, especially in a patient with previous head trauma, suggest subdural infection. Treatment consists of drainage and systemic antibiotics. These case reports demonstrate the clinical features of this rarely reported entity, and especially illustrate the need for careful bacteriologic identification in suspected cases."}
{"id": "PMID:1093404", "title": "Calcitonin levels in thyroid-vein blood of man.", "content": "Radioimmunoassay determinations of calcitonin from blood samples obtained by selective venous catheterization have demonstrated a large peripheral to thyroid vein gradient for this hormone in both normocalcemic and hypercalcemic persons. These fingings indicate that in fasting humans the thyroid gland maintains peripheral levels of calcitonin by actively secreting the hormone, and are consistent with the suggestion that the role of plasma calcitonin is of physiologic significance.", "contents": "Calcitonin levels in thyroid-vein blood of man. Radioimmunoassay determinations of calcitonin from blood samples obtained by selective venous catheterization have demonstrated a large peripheral to thyroid vein gradient for this hormone in both normocalcemic and hypercalcemic persons. These fingings indicate that in fasting humans the thyroid gland maintains peripheral levels of calcitonin by actively secreting the hormone, and are consistent with the suggestion that the role of plasma calcitonin is of physiologic significance."}
{"id": "PMID:1093405", "title": "The prophylaxis of gonorrhea.", "content": "The current level of the gonorrhea epidemic supports the need for additional measures for gonorrhea control. In the absence of evidence that prophylactic availability enhances promiscuity, a strong case can be made for venereal disease prophylaxis. This paper reviews the efficacy and acceptability of prophylactic techniques. In the military, acceptability and efficacy have been demonstrated for systemic antibiotics, and probably a postcoital prophylactic kit. These measures are either inappropriate or unavailable for general civilian use. The condom and vaginal spermicides offer promise of partial protection; further studies of efficacy and utilization are needed.", "contents": "The prophylaxis of gonorrhea. The current level of the gonorrhea epidemic supports the need for additional measures for gonorrhea control. In the absence of evidence that prophylactic availability enhances promiscuity, a strong case can be made for venereal disease prophylaxis. This paper reviews the efficacy and acceptability of prophylactic techniques. In the military, acceptability and efficacy have been demonstrated for systemic antibiotics, and probably a postcoital prophylactic kit. These measures are either inappropriate or unavailable for general civilian use. The condom and vaginal spermicides offer promise of partial protection; further studies of efficacy and utilization are needed."}
{"id": "PMID:1093410", "title": "Comparison of results of microbiologic and radioisotopic assays for serum vitamin B12 during pregnancy.", "content": "The serum vitamin B12 level falls progressively during pregnancy, when measured either by microbiologic assay or by a radioisotope dilution technique. The concentration of the vitamin assayed by radioisotope dilution was consistently higher than the value obtained by microbiologic assay. This discrepancy was greatest in early pregnancy and decreased as the duration of pregnancy increased. These results add further evidence to the hypothesis that changes in serum binders for vitamin B12 are of major significance in the pathogenesis of the observed fall in serum levels of the vitamin during pregnancy.", "contents": "Comparison of results of microbiologic and radioisotopic assays for serum vitamin B12 during pregnancy. The serum vitamin B12 level falls progressively during pregnancy, when measured either by microbiologic assay or by a radioisotope dilution technique. The concentration of the vitamin assayed by radioisotope dilution was consistently higher than the value obtained by microbiologic assay. This discrepancy was greatest in early pregnancy and decreased as the duration of pregnancy increased. These results add further evidence to the hypothesis that changes in serum binders for vitamin B12 are of major significance in the pathogenesis of the observed fall in serum levels of the vitamin during pregnancy."}
{"id": "PMID:1093411", "title": "Urinary stress incontinence: differential diagnosis, pathophysiology, and management.", "content": "It is appropriate to introduce this review of the subject of urinary stress incontinence with a discussion of  other disorders that may produce abdnormal urinary leakage,at times even stimulating true anatomic stress incontinence. It is obviously important to correctly identify patients with these other conditions and not perform surgical procedures unlikely to relieve their symptoms. Thereafter, the underlying pathophysiology of stress incontinence is considered in detail, since its fundamental understanding serves as the basis for both accurate diagnosis and proper selection and effective execution of the operations designed to correct true anatomic stress incontinence. A final section on patient management covers choice of the optimal surgical procedure for each individual patient, end results that should be achievable by proper selection and performance of the various operations, and a descriotion of some of the technical details that seem particularly helpful in carrying out the three principal types of surgical procedres currently in use.", "contents": "Urinary stress incontinence: differential diagnosis, pathophysiology, and management. It is appropriate to introduce this review of the subject of urinary stress incontinence with a discussion of  other disorders that may produce abdnormal urinary leakage,at times even stimulating true anatomic stress incontinence. It is obviously important to correctly identify patients with these other conditions and not perform surgical procedures unlikely to relieve their symptoms. Thereafter, the underlying pathophysiology of stress incontinence is considered in detail, since its fundamental understanding serves as the basis for both accurate diagnosis and proper selection and effective execution of the operations designed to correct true anatomic stress incontinence. A final section on patient management covers choice of the optimal surgical procedure for each individual patient, end results that should be achievable by proper selection and performance of the various operations, and a descriotion of some of the technical details that seem particularly helpful in carrying out the three principal types of surgical procedres currently in use."}
{"id": "PMID:1093413", "title": "Visual acuity improvement in eyes with corneal scars fitted with contact lenses.", "content": "The vision of 190 eyes of 130 patients with corneal scars was tested with contact lenses, of which 166 eyes subsequently were fitted with contact lenses. Over 75% of all eyes with corneal scars showed a visual acuity improvement to 6/12 or better. All eyes with a nebular or macular corneal scar and an initial visual acuity of 6/60 or better improved to 6/12 or better. The visual acuity of 77% of the eyes fitted with contact lenses, compared to the visual acuity of 36% of the same eyes obtained with spectacle lenses, improved to 6/12 or better.", "contents": "Visual acuity improvement in eyes with corneal scars fitted with contact lenses. The vision of 190 eyes of 130 patients with corneal scars was tested with contact lenses, of which 166 eyes subsequently were fitted with contact lenses. Over 75% of all eyes with corneal scars showed a visual acuity improvement to 6/12 or better. All eyes with a nebular or macular corneal scar and an initial visual acuity of 6/60 or better improved to 6/12 or better. The visual acuity of 77% of the eyes fitted with contact lenses, compared to the visual acuity of 36% of the same eyes obtained with spectacle lenses, improved to 6/12 or better."}
{"id": "PMID:1093415", "title": "Baroreceptor mechanisms controlling sympathetic nervous rhythms of central origin.", "content": "A study was made in the anesthetized cat of the slow wave of sympathetic nervous discharge (SND) locked in a 1:1 relation to the cardiac cycle (3 cycle/s periodicity). SND was recorded from the preganglionic splanchnic and postganglionic renal nerves. The data contradict the generally accepted view that the slow wave occurs as the direct result of a waxing and waning of baroreceptor nervous discharge. Although baroreceptor denervation unlocked the phase relations between SND and the cardiac cycle, the slow wave persisted and its duration was not changed. Furthermore, the slow wave was aborted by stimuli delivered to the baroreceptor nerves or paramedian reticular nucleus during a time span which accounted for less than 1 percent of the cardiac cycle. It is concluded that the 3 cycle/s periodicity of SND is representative of a vasomotor rhythm of central origin which is entrained to the cardiac cycle by the baroreceptor reflexes. The sympathoinhibitory effect leading to the entrainment of the slow wave is mediated in the brainstem. ta spinal component of baroreceptor-induced sympathoinhibition also was demonstrated.", "contents": "Baroreceptor mechanisms controlling sympathetic nervous rhythms of central origin. A study was made in the anesthetized cat of the slow wave of sympathetic nervous discharge (SND) locked in a 1:1 relation to the cardiac cycle (3 cycle/s periodicity). SND was recorded from the preganglionic splanchnic and postganglionic renal nerves. The data contradict the generally accepted view that the slow wave occurs as the direct result of a waxing and waning of baroreceptor nervous discharge. Although baroreceptor denervation unlocked the phase relations between SND and the cardiac cycle, the slow wave persisted and its duration was not changed. Furthermore, the slow wave was aborted by stimuli delivered to the baroreceptor nerves or paramedian reticular nucleus during a time span which accounted for less than 1 percent of the cardiac cycle. It is concluded that the 3 cycle/s periodicity of SND is representative of a vasomotor rhythm of central origin which is entrained to the cardiac cycle by the baroreceptor reflexes. The sympathoinhibitory effect leading to the entrainment of the slow wave is mediated in the brainstem. ta spinal component of baroreceptor-induced sympathoinhibition also was demonstrated."}
{"id": "PMID:1093416", "title": "Effects of vagotomy on urecholine-modified histamine dose responses in dogs.", "content": "Histamine step-dose responses, 2-100 mug/kg-h in 6 successive 45-min steps, were studied in nine dogs with gastric cannula. In three of the dogs the gastric fundus was selectively vagally denervated and the dose responses were repeated. In both groups of dogs the dose responses were then studied against background intravenous infusions of urecholine (10, 20, 40, and 80 mug/kg-h). Vagotomy acted as a competitive inhibitor (Km doubled from 14 to 29 mug/kg-h, while Vmax was unchanged) and this effect could be reversed by smaller than 10 mug urecholine/kg-h. In both groups increasing backgrounds of urecholine increased Vmax from similar to 14 meq H+/30 min to similar to 23-24 meq/30 min and decreased histamine km from 14 mug/kg-h to about 1-2 mug/kg-h. Both changes were log linear. The effects of urecholine on histamine-stimulated H+ secretion thus showed synergism (decreased Km) and potentiation (increased Vmax), the former interpreted as a cholinergic effect on the parietal cell histamine receptors and the latter as the probable existence of spare cholinergic receptors not (normally) accessible to histamine. There were no intrinsic qualitative or quantitative differences in these responses between the vagotomized and the innervated stomachs.", "contents": "Effects of vagotomy on urecholine-modified histamine dose responses in dogs. Histamine step-dose responses, 2-100 mug/kg-h in 6 successive 45-min steps, were studied in nine dogs with gastric cannula. In three of the dogs the gastric fundus was selectively vagally denervated and the dose responses were repeated. In both groups of dogs the dose responses were then studied against background intravenous infusions of urecholine (10, 20, 40, and 80 mug/kg-h). Vagotomy acted as a competitive inhibitor (Km doubled from 14 to 29 mug/kg-h, while Vmax was unchanged) and this effect could be reversed by smaller than 10 mug urecholine/kg-h. In both groups increasing backgrounds of urecholine increased Vmax from similar to 14 meq H+/30 min to similar to 23-24 meq/30 min and decreased histamine km from 14 mug/kg-h to about 1-2 mug/kg-h. Both changes were log linear. The effects of urecholine on histamine-stimulated H+ secretion thus showed synergism (decreased Km) and potentiation (increased Vmax), the former interpreted as a cholinergic effect on the parietal cell histamine receptors and the latter as the probable existence of spare cholinergic receptors not (normally) accessible to histamine. There were no intrinsic qualitative or quantitative differences in these responses between the vagotomized and the innervated stomachs."}
{"id": "PMID:1093417", "title": "Geometrical distribution of capillaries in mammalian striated muscle.", "content": "Striated muscles of hindlimb, jaw, or tongue in dogs, cats, rabbits, rats, and guinea pigs were perfused under high pressure with Microfil (a silicone elastomer of viscosity 20 cP approx.) to outline the vascular bed. When the material had set, the muscles were fixed in formalin. Exmination of histological sections, strained by a modified Gomori trichrome method, showed capillaries containing Microfil as well as some, unperfused, filled with red cells. From well-perfused bundles the numbers of capillaries surrounding each fiber were counted; in all muscles these ranged from 0 to 9 with mean values betweeen 3.2 and 4.0. This was true of both red and white muscle. The mean number of fibers sharing one capillary ranged from 2.0 to 3.2, the lower value being found when four vessels surrounded a fiber and the higher value when there was only three. The results indicate a continuum of transitional models from a square array of fibers with each capillary between two adjacent fibers to a hexagonal array with capillaries at alternate vertices.", "contents": "Geometrical distribution of capillaries in mammalian striated muscle. Striated muscles of hindlimb, jaw, or tongue in dogs, cats, rabbits, rats, and guinea pigs were perfused under high pressure with Microfil (a silicone elastomer of viscosity 20 cP approx.) to outline the vascular bed. When the material had set, the muscles were fixed in formalin. Exmination of histological sections, strained by a modified Gomori trichrome method, showed capillaries containing Microfil as well as some, unperfused, filled with red cells. From well-perfused bundles the numbers of capillaries surrounding each fiber were counted; in all muscles these ranged from 0 to 9 with mean values betweeen 3.2 and 4.0. This was true of both red and white muscle. The mean number of fibers sharing one capillary ranged from 2.0 to 3.2, the lower value being found when four vessels surrounded a fiber and the higher value when there was only three. The results indicate a continuum of transitional models from a square array of fibers with each capillary between two adjacent fibers to a hexagonal array with capillaries at alternate vertices."}
{"id": "PMID:1093421", "title": "Protection of the ischemic liver by donor pretreatment before transplantation.", "content": "Canine livers ischemically damaged for thirty minutes prior to auxiliary transplantation did not survive for long periods of time unless a combination of isoproterenol, allopurinol, and heparin was administered intravenously to the donor animal before the ischemic damage. These drugs had no protective effect when given individually. Ischemic livers treated with adenosine prior to transplantation also showed no improved recovery of function.", "contents": "Protection of the ischemic liver by donor pretreatment before transplantation. Canine livers ischemically damaged for thirty minutes prior to auxiliary transplantation did not survive for long periods of time unless a combination of isoproterenol, allopurinol, and heparin was administered intravenously to the donor animal before the ischemic damage. These drugs had no protective effect when given individually. Ischemic livers treated with adenosine prior to transplantation also showed no improved recovery of function."}
{"id": "PMID:1093422", "title": "Lethal complications of bilateral nephrectomy and splenectomy in hemodialyzed patients.", "content": "Routine bilateral nephrectomy and splenectomy (BNS) in uremic patients before transplantation are relatively safe procedures except when there is pre-existing sepsis, diabetes, or severe hypertension. A review of 421 patients undergoing routine pretransplantation BNS reveals that death before transplantation occurs in two definable groups of patients. In our series, the first group, those with juvenile onset diabetes, have a 15.4 per cent pretransplantation mortality (9.6 per cent operative and 5.8 per cent nonoperative) while being maintained on hemodialysis and awaiting transplantation. The second group, nondiabetic patients with other preoperatively definable risk factors such as severe hypertension and infected kidneys, had a 3.25 per cent pretransplantation mortality (1.9 per cent operative and 1.25 per cent nonoperative) while on hemodialysis. Paradoxically, these same factors are used as absolute criteria for pretransplantation nephrectomy at institutions where this operation is not a routine part of the pretransplantation regimen.", "contents": "Lethal complications of bilateral nephrectomy and splenectomy in hemodialyzed patients. Routine bilateral nephrectomy and splenectomy (BNS) in uremic patients before transplantation are relatively safe procedures except when there is pre-existing sepsis, diabetes, or severe hypertension. A review of 421 patients undergoing routine pretransplantation BNS reveals that death before transplantation occurs in two definable groups of patients. In our series, the first group, those with juvenile onset diabetes, have a 15.4 per cent pretransplantation mortality (9.6 per cent operative and 5.8 per cent nonoperative) while being maintained on hemodialysis and awaiting transplantation. The second group, nondiabetic patients with other preoperatively definable risk factors such as severe hypertension and infected kidneys, had a 3.25 per cent pretransplantation mortality (1.9 per cent operative and 1.25 per cent nonoperative) while on hemodialysis. Paradoxically, these same factors are used as absolute criteria for pretransplantation nephrectomy at institutions where this operation is not a routine part of the pretransplantation regimen."}
{"id": "PMID:1093423", "title": "Gastroenteric anastomosis in Billroth II gastrectomy with maintenance of the physiologic diameter of the normal pylorus to prevent the dumping syndrome. Part II. Results in 1,300 cases.", "content": "Clinical results of the use of a gastroenteric anastomosis with a diameter the size of the normal pylorus (1.9 cm) in 1,300 cases between 1954 and 1971 are presented mainly in terms of prevention of the dumping syndrome. We have had no patient with clinical manifestations of any degree of the dumping syndrome in the first two years after operation. These excellent results have led us to employ the method routinely. We recommend this method for its simplicity and, above all, its effectiveness in preventing the dumping syndrome.", "contents": "Gastroenteric anastomosis in Billroth II gastrectomy with maintenance of the physiologic diameter of the normal pylorus to prevent the dumping syndrome. Part II. Results in 1,300 cases. Clinical results of the use of a gastroenteric anastomosis with a diameter the size of the normal pylorus (1.9 cm) in 1,300 cases between 1954 and 1971 are presented mainly in terms of prevention of the dumping syndrome. We have had no patient with clinical manifestations of any degree of the dumping syndrome in the first two years after operation. These excellent results have led us to employ the method routinely. We recommend this method for its simplicity and, above all, its effectiveness in preventing the dumping syndrome."}
{"id": "PMID:1093424", "title": "Decreased function of a renal transplant after influenza virus infection.", "content": "A sixteen year old female recipient of a renal transplant had decreased renal function one month after an influenza virus infection. An acute rejection episode induced by the viral infection was the probable cause. Renal function in transplant recipients should be carefully monitored for at least one month after viral infection.", "contents": "Decreased function of a renal transplant after influenza virus infection. A sixteen year old female recipient of a renal transplant had decreased renal function one month after an influenza virus infection. An acute rejection episode induced by the viral infection was the probable cause. Renal function in transplant recipients should be carefully monitored for at least one month after viral infection."}
{"id": "PMID:1093425", "title": "Quantification of retinal lesions in diabetic retinopathy.", "content": "A method of quantitative evaluation of the number of microaneurysms is described for clinical research in the therapy of early stages of diabetic retinopathy. In a double-blind study it can be demonstrated that the described method quantifies the progression of retinopathy statistically during only one year of observation in 32 resp. 40 patients. The difficulties of quantification are due to interpretation problems of the photographic documentation and the learning problem of the observer.", "contents": "Quantification of retinal lesions in diabetic retinopathy. A method of quantitative evaluation of the number of microaneurysms is described for clinical research in the therapy of early stages of diabetic retinopathy. In a double-blind study it can be demonstrated that the described method quantifies the progression of retinopathy statistically during only one year of observation in 32 resp. 40 patients. The difficulties of quantification are due to interpretation problems of the photographic documentation and the learning problem of the observer."}
{"id": "PMID:1093426", "title": "The influence of the route of administration on the clinical action of diazepam.", "content": "The relative efficacy of diazepam 10 mg as a premedicant was assessed following its oral and intramuscular administration, using a \"double-blind, double-dummy\" technique, as well as its effect when given by both routes. Drugs were given to a standard patient population who were undergoing the same type of operation and receiving a standard anaesthetic. The most rapid onset of soporific action occurred when diazepam was given by mouth and this produced the best effect throughout the period of the study although its efficacy began to pass off after 60 minutes. It was superior in effect to the same dose injected into the thigh. The combined effect of oral and intramuscular diazepam was not notably greater than tht of the single oral dose. The injection of diazepam was followed by an unacceptably high incidence of pain, particularly when this was given into the thigh. There were no significant differences in either the course of anaesthesia or the incidence of post-operative nausea and vomiting. When the undesired effects are taken into consideration, the oral administration of diazepam should be the route of choice.", "contents": "The influence of the route of administration on the clinical action of diazepam. The relative efficacy of diazepam 10 mg as a premedicant was assessed following its oral and intramuscular administration, using a \"double-blind, double-dummy\" technique, as well as its effect when given by both routes. Drugs were given to a standard patient population who were undergoing the same type of operation and receiving a standard anaesthetic. The most rapid onset of soporific action occurred when diazepam was given by mouth and this produced the best effect throughout the period of the study although its efficacy began to pass off after 60 minutes. It was superior in effect to the same dose injected into the thigh. The combined effect of oral and intramuscular diazepam was not notably greater than tht of the single oral dose. The injection of diazepam was followed by an unacceptably high incidence of pain, particularly when this was given into the thigh. There were no significant differences in either the course of anaesthesia or the incidence of post-operative nausea and vomiting. When the undesired effects are taken into consideration, the oral administration of diazepam should be the route of choice."}
{"id": "PMID:1093427", "title": "Plasma diazepam levels after single dose oral and intramuscular administration.", "content": "Plasma diazepam levels were estimated by gas-liquid chromatography following a single 10 mg dose administered to a health adult female population by the oral and intramuscular routes. The highest levels were achieved following oral administration, reaching a peak at 60 minutes, although injection into the thigh resulted in a more rapid rise initially. Injection into this site produced higher levels than when the buttock was used. Injection by nurses into the latter site produced particularly low plasma levels over the 90-minute period of study. The plasma levels found at 90 minutes following the oral and thigh routes were found to be closely correlated with the weight of the patient.", "contents": "Plasma diazepam levels after single dose oral and intramuscular administration. Plasma diazepam levels were estimated by gas-liquid chromatography following a single 10 mg dose administered to a health adult female population by the oral and intramuscular routes. The highest levels were achieved following oral administration, reaching a peak at 60 minutes, although injection into the thigh resulted in a more rapid rise initially. Injection into this site produced higher levels than when the buttock was used. Injection by nurses into the latter site produced particularly low plasma levels over the 90-minute period of study. The plasma levels found at 90 minutes following the oral and thigh routes were found to be closely correlated with the weight of the patient."}
{"id": "PMID:1093428", "title": "Adrenocortical function related to Althesin anaesthesia and surgery in man.", "content": "Althesin anaesthesia alone had no significant effect on plasma cortisol concentration. When Althesin induction was followed by maintenance with N2O and halothane there was a statistically insignificant fall, but with N2O, pancuronium and I.P.P.V there was a tendency for plasma cortisol concentrations to rise. This, too, was statistically not significant. A significant elevation in the mean plasma cortisol concentration was only found in patients during abdominal surgery.", "contents": "Adrenocortical function related to Althesin anaesthesia and surgery in man. Althesin anaesthesia alone had no significant effect on plasma cortisol concentration. When Althesin induction was followed by maintenance with N2O and halothane there was a statistically insignificant fall, but with N2O, pancuronium and I.P.P.V there was a tendency for plasma cortisol concentrations to rise. This, too, was statistically not significant. A significant elevation in the mean plasma cortisol concentration was only found in patients during abdominal surgery."}
{"id": "PMID:1093429", "title": "Positive end-expiratory pressure. Effect on arterial oxygenation during respiratory failure in chronic obstructive airway disease.", "content": "Changes induced in arterial oxygenation by positive end-expiratory pressure (PEEP) were analysed in twenty-nine instances in seven patients with chronic obstructive airway disease and acute respiratory failure, who were receiving IPPV. A significant decrease in mean PaO2 was found 4 hours after the removal of PEEP (P smaller than 0-01), but after its addition during the same period the PaO2 change was not significant. PaCO2 values were not modified by PEEP. The slight increase in mean PaO2 (20-9 mmHg) encountered during controlled ventilation with PEEP seems of doubtful clinical value. Alveolar-arterial oxygen gradient, while breathing oxygen 100%, was found to be elevated with a mean value of 276 mmHg. It appears from our results that PEEP is of little or no value in the routine ventilatory treatment of patients with chronic obstructive airway disease unless hypoxaemia is believed to be caused by a large intrapulmonary shunt.", "contents": "Positive end-expiratory pressure. Effect on arterial oxygenation during respiratory failure in chronic obstructive airway disease. Changes induced in arterial oxygenation by positive end-expiratory pressure (PEEP) were analysed in twenty-nine instances in seven patients with chronic obstructive airway disease and acute respiratory failure, who were receiving IPPV. A significant decrease in mean PaO2 was found 4 hours after the removal of PEEP (P smaller than 0-01), but after its addition during the same period the PaO2 change was not significant. PaCO2 values were not modified by PEEP. The slight increase in mean PaO2 (20-9 mmHg) encountered during controlled ventilation with PEEP seems of doubtful clinical value. Alveolar-arterial oxygen gradient, while breathing oxygen 100%, was found to be elevated with a mean value of 276 mmHg. It appears from our results that PEEP is of little or no value in the routine ventilatory treatment of patients with chronic obstructive airway disease unless hypoxaemia is believed to be caused by a large intrapulmonary shunt."}
{"id": "PMID:1093430", "title": "Pneumopericardium associated with tracheal rupture. Management of a case complicated by the shock lung syndrome.", "content": "A case history of a patient with unusual injuries following blunt thoracic trauma is presented. There was a pneumopericardium, bilateral tension pneumothoraces and rupture of the thoracic trachea, and the shock lung syndrome developed. Some points which arose in the management of the case are discussed, and it is pointed out that such injuries may be missed and must be considered in all patients with thoracic trauma especially those who do not improve with routine therapy.", "contents": "Pneumopericardium associated with tracheal rupture. Management of a case complicated by the shock lung syndrome. A case history of a patient with unusual injuries following blunt thoracic trauma is presented. There was a pneumopericardium, bilateral tension pneumothoraces and rupture of the thoracic trachea, and the shock lung syndrome developed. Some points which arose in the management of the case are discussed, and it is pointed out that such injuries may be missed and must be considered in all patients with thoracic trauma especially those who do not improve with routine therapy."}
{"id": "PMID:1093431", "title": "The immediate care of head injuries.", "content": "Anaesthetists have much to contribute as members of a team concerned with the management of a severe head injury. Many factors have been discussed but it must be remembered that their most important duty is to maintain a clear airway.", "contents": "The immediate care of head injuries. Anaesthetists have much to contribute as members of a team concerned with the management of a severe head injury. Many factors have been discussed but it must be remembered that their most important duty is to maintain a clear airway."}
{"id": "PMID:1093432", "title": "Chloroform at Christmas. An early reference from the Theatre-Royal, Edinburgh.", "content": "James Young Simpson first administered chloroform to one of his obstetric patients on 5 November 1847. The speed with which the discovery caught the popular imagination is illustrated by a comic scene in a pantomime which formed part of an entertainment in Edinburgh presented on 27 December in the same year.", "contents": "Chloroform at Christmas. An early reference from the Theatre-Royal, Edinburgh. James Young Simpson first administered chloroform to one of his obstetric patients on 5 November 1847. The speed with which the discovery caught the popular imagination is illustrated by a comic scene in a pantomime which formed part of an entertainment in Edinburgh presented on 27 December in the same year."}
{"id": "PMID:1093433", "title": "Acute opium poisoning: a report of two hundred cases in Iran.", "content": "The diagnosis and management of 200 cases of self-poisoning due to orally ingested crude opium in Iran is reported and discussed. Gastric lavage supported when necessary by nalorphine was effective treatment in the majority of cases because of the slow absorption of opium. Mechanical ventilation was required in twelve cases (6%) only. There were three deaths; two of these were due to pulmonary oedema which was apparently not the result of hypoxia.", "contents": "Acute opium poisoning: a report of two hundred cases in Iran. The diagnosis and management of 200 cases of self-poisoning due to orally ingested crude opium in Iran is reported and discussed. Gastric lavage supported when necessary by nalorphine was effective treatment in the majority of cases because of the slow absorption of opium. Mechanical ventilation was required in twelve cases (6%) only. There were three deaths; two of these were due to pulmonary oedema which was apparently not the result of hypoxia."}
{"id": "PMID:1093436", "title": "[Clinical investigations of the steroid anaesthetic agent CT-1341 (Althesin) (Cardiovascular and respiratory effects)].", "content": "The new steroid anaesthetic CT-1341 (Althesin) has been used as an induction agent in dose of 0.07 ml/kg body weight. Following injection, stage 1 anaesthesia is reached in an average of 30 sec with a clinical duration of between 12 and 20 min with total recovery, supported by a normal pre-anaesthetic EEG tracing, in 40 to 50 min. The respiratory depression following injection in characterized by a short period of apnea followed by an increased respiratory rate and a diminished tidal volume; there is a decrease of the pO2 of about 15%, but the pCO2 and pH values do not show significant variations. Concerning its action on the cardiovascular system, there was found an increase of heart rate (+14%), a decrease of both systolic (-19%) and diastolic pressures (minus decrease of both systolic (-19%) and diastolic pressure (-13.5%), central venous pressure and the peripheral vascular resistance (-14%) with hardly any change in the pulmonary artery pressure.", "contents": "[Clinical investigations of the steroid anaesthetic agent CT-1341 (Althesin) (Cardiovascular and respiratory effects)]. The new steroid anaesthetic CT-1341 (Althesin) has been used as an induction agent in dose of 0.07 ml/kg body weight. Following injection, stage 1 anaesthesia is reached in an average of 30 sec with a clinical duration of between 12 and 20 min with total recovery, supported by a normal pre-anaesthetic EEG tracing, in 40 to 50 min. The respiratory depression following injection in characterized by a short period of apnea followed by an increased respiratory rate and a diminished tidal volume; there is a decrease of the pO2 of about 15%, but the pCO2 and pH values do not show significant variations. Concerning its action on the cardiovascular system, there was found an increase of heart rate (+14%), a decrease of both systolic (-19%) and diastolic pressures (minus decrease of both systolic (-19%) and diastolic pressure (-13.5%), central venous pressure and the peripheral vascular resistance (-14%) with hardly any change in the pulmonary artery pressure."}
{"id": "PMID:1093437", "title": "Radial artery cannulation.", "content": "The status of radial artery cannulation is reviewed. The technique of percutaneous puncture, equipment in use, and known complications are discussed. Recommendations are made for precautionary measures to reduce the complication rate. It is concluded that radial artery cannulation is a safe and useful procedure in certain anaesthetic and intensive care situations.", "contents": "Radial artery cannulation. The status of radial artery cannulation is reviewed. The technique of percutaneous puncture, equipment in use, and known complications are discussed. Recommendations are made for precautionary measures to reduce the complication rate. It is concluded that radial artery cannulation is a safe and useful procedure in certain anaesthetic and intensive care situations."}
{"id": "PMID:1093438", "title": "Management of tracheal rupture involving both bronchi.", "content": "A patient with a rupture of the lower trachea and both bronchi is presented. A review of the significant clinical findings is presented, and the anaesthetic management is discussed. The necessity of early bronchoscopy in these patients and the ready availability of experienced medical staff in assessing all severe trauma is emphasized.", "contents": "Management of tracheal rupture involving both bronchi. A patient with a rupture of the lower trachea and both bronchi is presented. A review of the significant clinical findings is presented, and the anaesthetic management is discussed. The necessity of early bronchoscopy in these patients and the ready availability of experienced medical staff in assessing all severe trauma is emphasized."}
{"id": "PMID:1093439", "title": "Evaluation of infant ventilators.", "content": "A method of evaluating the performance of paediatric ventilators under simulated clinical conditions is described, together with the results of this evaluation of the prototypes of two ventilators designed specifically for use with infants and small children.", "contents": "Evaluation of infant ventilators. A method of evaluating the performance of paediatric ventilators under simulated clinical conditions is described, together with the results of this evaluation of the prototypes of two ventilators designed specifically for use with infants and small children."}
{"id": "PMID:1093453", "title": "Teaching effectiveness: evaluation of computer-assisted instruction for cardiopulmonary resuscitation.", "content": "Computer instruction for cardiopulmonary resuscitation (CPR), using a case-simulation program, was evaluated for its teaching effectiveness. First-month anesthesia residents, divided into two groups, A and B, underwent a cognitive evaluation on their 1st day of arrival and again on their 5th day. Both groups were provided with clear educational objectives and reading materials, a tutor was assigned to each student to answer specific questions. Students in group A, also given computer-assisted instruction (CAI), showed a significantly higher learning than those in group B, who served as controls. That CAI was a highly motivational form of learning was established by later voluntary participation in CAI of all group B students.", "contents": "Teaching effectiveness: evaluation of computer-assisted instruction for cardiopulmonary resuscitation. Computer instruction for cardiopulmonary resuscitation (CPR), using a case-simulation program, was evaluated for its teaching effectiveness. First-month anesthesia residents, divided into two groups, A and B, underwent a cognitive evaluation on their 1st day of arrival and again on their 5th day. Both groups were provided with clear educational objectives and reading materials, a tutor was assigned to each student to answer specific questions. Students in group A, also given computer-assisted instruction (CAI), showed a significantly higher learning than those in group B, who served as controls. That CAI was a highly motivational form of learning was established by later voluntary participation in CAI of all group B students."}
{"id": "PMID:1093458", "title": "Psychomotor skills related to driving after intramuscular administration of diazepam and meperidine.", "content": "Psychomotor skills related to driving and the ability to discriminate the fusion of flickering light were measured in a double-blind cross-over fashion in 11 healthy volunteers before, and 1, 3, 5, and 7 hours after, intramuscular injection of saline solution, 10 mg diazepam, or 75 mg meperidine. The late effects of meperidine were tested in five other subjects 12 and 24 hours after the injection. The effects of diazepam were the most harmful to coordinative and reactive skills, which were significantly impaired for as long as 5 hours. Meperidine impaired reactive skills for as long as 3 hours and flicker-fusion discrimination and coordinative skills for as long as 12 hours. It is concluded that patients should not drive or operate machinery for at least 7 hours after receiving 10 mg diazepam intramuscularly and for 24 hours after receiving 75 mg meperidine intramuscularly.", "contents": "Psychomotor skills related to driving after intramuscular administration of diazepam and meperidine. Psychomotor skills related to driving and the ability to discriminate the fusion of flickering light were measured in a double-blind cross-over fashion in 11 healthy volunteers before, and 1, 3, 5, and 7 hours after, intramuscular injection of saline solution, 10 mg diazepam, or 75 mg meperidine. The late effects of meperidine were tested in five other subjects 12 and 24 hours after the injection. The effects of diazepam were the most harmful to coordinative and reactive skills, which were significantly impaired for as long as 5 hours. Meperidine impaired reactive skills for as long as 3 hours and flicker-fusion discrimination and coordinative skills for as long as 12 hours. It is concluded that patients should not drive or operate machinery for at least 7 hours after receiving 10 mg diazepam intramuscularly and for 24 hours after receiving 75 mg meperidine intramuscularly."}
{"id": "PMID:1093456", "title": "Sequential blood volume changes in patients undergoing total hip arthroplasty.", "content": "Adequacy of transfusion based exclusively on clinical observations of hemodynamic changes and quantities of blood lost during total hip arthroplasty (THA) in 15 adults was tested by serial measurements of 51-Cr red-cell volume (RCV), 125-I RISA plasma volume (PV), and peripheral hematocrit (Hv) determined immediately before and 2 and 48 hours after operation. Blood transfusion and intravenous-fluid therapy were based on clinical observations and designed to restore blood volume (BV) to within 10 percent of its preoperative level prior to the isotopic determination 2 hours postoperatively. (When that determination showed a BV less than 90 percent, transfusions were given to restore it to between 90 and 100 percent). Isotopic data 2 hours after operation showed that the BV reduction--thought clinically to be less than 10 percent in every case--averaged 21.6 percent (range: 6 to 39 percent) and was shared by RCV and PV. Isotopic measurements 48 hours after operation showed that patients whose BV at 2 hours was within 10 percent of the preoperative BV were able to compensate for an average continuing RCV reduction of 23.9 percent by expansion of PV, resulting in an average BV reduction of 11.4 percent. Hv was a valuable guide for transfusion therapy at 48 hours, but not 2 hours after operation. In view of inadequacy of blood replacement based on clinical observations and the magnitude and variability of the 2 hour postoperative isotopically measured BV reductions in our patients, we recommend obtaining a derived BV by measuring PV and Hv preoperatively and 2 hours postoperatively as a further guide to transfusion therapy for each patient under-going THA. This is simpler and more practical than the two-isotope technique and is more sensitive than clinical observation alone. If clinical observation had been supplemented with a derived BV in our 15 cases, 80 percent instead of 30 percent would have had their BV restored to within 10 percent of the preoperative BV.", "contents": "Sequential blood volume changes in patients undergoing total hip arthroplasty. Adequacy of transfusion based exclusively on clinical observations of hemodynamic changes and quantities of blood lost during total hip arthroplasty (THA) in 15 adults was tested by serial measurements of 51-Cr red-cell volume (RCV), 125-I RISA plasma volume (PV), and peripheral hematocrit (Hv) determined immediately before and 2 and 48 hours after operation. Blood transfusion and intravenous-fluid therapy were based on clinical observations and designed to restore blood volume (BV) to within 10 percent of its preoperative level prior to the isotopic determination 2 hours postoperatively. (When that determination showed a BV less than 90 percent, transfusions were given to restore it to between 90 and 100 percent). Isotopic data 2 hours after operation showed that the BV reduction--thought clinically to be less than 10 percent in every case--averaged 21.6 percent (range: 6 to 39 percent) and was shared by RCV and PV. Isotopic measurements 48 hours after operation showed that patients whose BV at 2 hours was within 10 percent of the preoperative BV were able to compensate for an average continuing RCV reduction of 23.9 percent by expansion of PV, resulting in an average BV reduction of 11.4 percent. Hv was a valuable guide for transfusion therapy at 48 hours, but not 2 hours after operation. In view of inadequacy of blood replacement based on clinical observations and the magnitude and variability of the 2 hour postoperative isotopically measured BV reductions in our patients, we recommend obtaining a derived BV by measuring PV and Hv preoperatively and 2 hours postoperatively as a further guide to transfusion therapy for each patient under-going THA. This is simpler and more practical than the two-isotope technique and is more sensitive than clinical observation alone. If clinical observation had been supplemented with a derived BV in our 15 cases, 80 percent instead of 30 percent would have had their BV restored to within 10 percent of the preoperative BV."}
{"id": "PMID:1093459", "title": "Hyaline membrane disease.", "content": "In addition to confirmation of earlier epidemiologic observations, major advances have occurred in the past few years with respect to the diagnosis and treatment of hyaline membrane disease. Prenatal assessment of the risk for developing the disorder is now routinely possible by amniocentesis and analysis of the ratio of lecithin to sphingomyelin in amniotic fluid, or by surfactant titer (\"shake test\"). Such predictability and numerous other lines of evidence provide strong support for the hypothesis that hyaline membrane disease fundamentally reflects a deficiency of pulmonary surfactant. In accordance with the apparent primacy of surfactant deficiency, recent improvements in management are directed toward either increasing lung volume at end expiration by application of continuous distending airway pressure, or accelerating fetal lung maturation, with respect to the synthesis and secretion of surfactant, by prenatal administration of glucocorticoids.", "contents": "Hyaline membrane disease. In addition to confirmation of earlier epidemiologic observations, major advances have occurred in the past few years with respect to the diagnosis and treatment of hyaline membrane disease. Prenatal assessment of the risk for developing the disorder is now routinely possible by amniocentesis and analysis of the ratio of lecithin to sphingomyelin in amniotic fluid, or by surfactant titer (\"shake test\"). Such predictability and numerous other lines of evidence provide strong support for the hypothesis that hyaline membrane disease fundamentally reflects a deficiency of pulmonary surfactant. In accordance with the apparent primacy of surfactant deficiency, recent improvements in management are directed toward either increasing lung volume at end expiration by application of continuous distending airway pressure, or accelerating fetal lung maturation, with respect to the synthesis and secretion of surfactant, by prenatal administration of glucocorticoids."}
{"id": "PMID:1093460", "title": "The activity of chaulmoogra acids against Mycobacterium leprae.", "content": "The activity of the crude sodium salts of the fatty acids of chaulmoogra oil and of hydnocarpic and chaulmoogric acids against Mycobacterium leprae was studied in mouse footpad infection. Multiplication of the organisms was inhibited when the salts were administered intraperitoneally and subcutaneously 3 times per week, and when chaulmoogric acid was administered intraperitoneally 5 times per week in half the equivalent dose. Dihydrochaulmoogric acid was also active, whereas palmitic acid was not. Hydnocarpic acid administered intraperitoneally once per week in a dose equivalent to half that of the sodium salts of the chaulmoogra fatty acids was not effective. The demonstration that chaulmoogra fatty acids possess activity against M. leprae lends weight to our earlier suggestion that a study of compounds analogous to these acids may yield effective antimicrobial agents with a unique mechanism of action.", "contents": "The activity of chaulmoogra acids against Mycobacterium leprae. The activity of the crude sodium salts of the fatty acids of chaulmoogra oil and of hydnocarpic and chaulmoogric acids against Mycobacterium leprae was studied in mouse footpad infection. Multiplication of the organisms was inhibited when the salts were administered intraperitoneally and subcutaneously 3 times per week, and when chaulmoogric acid was administered intraperitoneally 5 times per week in half the equivalent dose. Dihydrochaulmoogric acid was also active, whereas palmitic acid was not. Hydnocarpic acid administered intraperitoneally once per week in a dose equivalent to half that of the sodium salts of the chaulmoogra fatty acids was not effective. The demonstration that chaulmoogra fatty acids possess activity against M. leprae lends weight to our earlier suggestion that a study of compounds analogous to these acids may yield effective antimicrobial agents with a unique mechanism of action."}
{"id": "PMID:1093461", "title": "Evaluation of mini-dose heparin administration as a prophylaxis against postoperative pulmonary embolism: a prospective double-blind study.", "content": "A prospective double-blind study was instituted in a group of 150 general surgical patients to test the effectiveness of mini-dose heparinization in the pre- and postoperative periods. There was a 21 per cent reduction in the incidence of deep venous thrombosis in the heparin treated group. A radiopharmaceutical imaging technique with 99m-technetium macroaggregated albumin was used to evaluate the deep venous system. The procedure proved to be simple, safe, and painless; however, it was difficult to differentiate venous stasis from deep venous thrombosis. A negative study was good evidence that deep venous thrombosis did not exist. An additional benefit of this procedure was that a perfusion lung study could be obtained which provided additional information regarding pulmonary embolism without injecting additional radiopharmaceutical. Again, the negative perfusion lung study provided more information.", "contents": "Evaluation of mini-dose heparin administration as a prophylaxis against postoperative pulmonary embolism: a prospective double-blind study. A prospective double-blind study was instituted in a group of 150 general surgical patients to test the effectiveness of mini-dose heparinization in the pre- and postoperative periods. There was a 21 per cent reduction in the incidence of deep venous thrombosis in the heparin treated group. A radiopharmaceutical imaging technique with 99m-technetium macroaggregated albumin was used to evaluate the deep venous system. The procedure proved to be simple, safe, and painless; however, it was difficult to differentiate venous stasis from deep venous thrombosis. A negative study was good evidence that deep venous thrombosis did not exist. An additional benefit of this procedure was that a perfusion lung study could be obtained which provided additional information regarding pulmonary embolism without injecting additional radiopharmaceutical. Again, the negative perfusion lung study provided more information."}
{"id": "PMID:1093464", "title": "Dimethyl sulfoxide therapy in nonmongoloid infantile oligophrenia.", "content": "We are herein submitting the results obtained with a group of 13 nonmongoloid, oligophrenic children after 180 days' treatment with DAT, and we have compared the results with those of a control group that received only conventional treatment. The children were examined before and after the treatment by means of the Binet-Simon tests, which have been standardized by Kuhlmann, and the Wise and Terman Merrill test. The control of organic lesion was determined by the Bender test. The variation mean of the control group was 0.08, and that of the DAT-treated group was 0.954; 0.874 was the mean difference, and 0.543 and 1.205 were the degrees of reliability. The difference between the results obtained from the DAT-treated group and the one that received conventional medication was significant (P equal 0.01). None of the 13 cases treated showed any signs of toxicity.", "contents": "Dimethyl sulfoxide therapy in nonmongoloid infantile oligophrenia. We are herein submitting the results obtained with a group of 13 nonmongoloid, oligophrenic children after 180 days' treatment with DAT, and we have compared the results with those of a control group that received only conventional treatment. The children were examined before and after the treatment by means of the Binet-Simon tests, which have been standardized by Kuhlmann, and the Wise and Terman Merrill test. The control of organic lesion was determined by the Bender test. The variation mean of the control group was 0.08, and that of the DAT-treated group was 0.954; 0.874 was the mean difference, and 0.543 and 1.205 were the degrees of reliability. The difference between the results obtained from the DAT-treated group and the one that received conventional medication was significant (P equal 0.01). None of the 13 cases treated showed any signs of toxicity."}
{"id": "PMID:1093465", "title": "Pharmacologic and biochemical considerations of dimethyl sulfoxide.", "content": "DMSO has multiple known pharmacological properties. In addition to those referred to include: cryoprotective action, radioprotective effect, influence on serum cholesterol in experimental hypercholesteremia, and platelet aggregation antagonism. How the multiple properties of this chemical affect therapy in clinical medicine must yet be explained. It is clear, however, that DMSO does affect biological systems, and that this influence has many clinical applications. Perhaps one of the most interesting and significant properties of DMSO is its ability to move other drugsthrough membranes. When mixed with DMSO, many drugs appear to be potentiated in their physiologic effect; thus smaller doses are required and less toxicity is demonstrated. In cancer chemotherapy, this value already has practical use. Some of the studies to be reported in this monograph will describe additional, almost unbelievable observations. Perhaps the mechanism of action of these clinical phenomena will be found in one or more of the pharmacological properties described. It would not be surprising, however, if we were to conclude with a resolution to search for new explantations of the mystery of DMSO, for it would appear that DMSO is really a new principle in medicine and cannot always be measured by existing standards.", "contents": "Pharmacologic and biochemical considerations of dimethyl sulfoxide. DMSO has multiple known pharmacological properties. In addition to those referred to include: cryoprotective action, radioprotective effect, influence on serum cholesterol in experimental hypercholesteremia, and platelet aggregation antagonism. How the multiple properties of this chemical affect therapy in clinical medicine must yet be explained. It is clear, however, that DMSO does affect biological systems, and that this influence has many clinical applications. Perhaps one of the most interesting and significant properties of DMSO is its ability to move other drugsthrough membranes. When mixed with DMSO, many drugs appear to be potentiated in their physiologic effect; thus smaller doses are required and less toxicity is demonstrated. In cancer chemotherapy, this value already has practical use. Some of the studies to be reported in this monograph will describe additional, almost unbelievable observations. Perhaps the mechanism of action of these clinical phenomena will be found in one or more of the pharmacological properties described. It would not be surprising, however, if we were to conclude with a resolution to search for new explantations of the mystery of DMSO, for it would appear that DMSO is really a new principle in medicine and cannot always be measured by existing standards."}
{"id": "PMID:1093467", "title": "Examination of lymphocyte membranes of athymic \"nude\" mice by scanning electron microscopy.", "content": "Recent reports 1-3 have proposed that T-lymphocytes (thymus derived) could be distinguished from B-lymphocytes (thymus independent) by examining their features under the scanning electron microscope. T-cells were designated as having relatively smooth surfaced cells, whereas B-cells had \"hairy\" surfaces with many microvilli. We have examined this hypothesis in congenitally athymic \"nude\" mice, animals lacking T-cells,4 and have not been able to confirm these reports. We have found that lymphocytes from nude (nu/nu) mice are indistinguishable from lymphocytes obtained from normal littermates (NLM) and CBA/J mice. We have found that in all the murine lymphoid tissue examined, including nude, the complete gamut of cell surface types, ranging from smooth to \"hairy\" are present. Our studies indicate that the proposed T- and B-cell classification based upon human surface morphology under the scanning electron microscope is questionable in a mouse model. It is presently unclear whether the smooth lymphocytes seen in nude mice are immature B-cells, pre-T-cells, or another unidentified population of cells.", "contents": "Examination of lymphocyte membranes of athymic \"nude\" mice by scanning electron microscopy. Recent reports 1-3 have proposed that T-lymphocytes (thymus derived) could be distinguished from B-lymphocytes (thymus independent) by examining their features under the scanning electron microscope. T-cells were designated as having relatively smooth surfaced cells, whereas B-cells had \"hairy\" surfaces with many microvilli. We have examined this hypothesis in congenitally athymic \"nude\" mice, animals lacking T-cells,4 and have not been able to confirm these reports. We have found that lymphocytes from nude (nu/nu) mice are indistinguishable from lymphocytes obtained from normal littermates (NLM) and CBA/J mice. We have found that in all the murine lymphoid tissue examined, including nude, the complete gamut of cell surface types, ranging from smooth to \"hairy\" are present. Our studies indicate that the proposed T- and B-cell classification based upon human surface morphology under the scanning electron microscope is questionable in a mouse model. It is presently unclear whether the smooth lymphocytes seen in nude mice are immature B-cells, pre-T-cells, or another unidentified population of cells."}
{"id": "PMID:1093469", "title": "Antigenic and functional evidence for the in vitro inductive activity of thymopoietin (thymin) on thymocyte precursors.", "content": "Thymopoietin, a polypeptide hormone isolated from bovine thymus, induced in vitro the differentiation of prothymocytes to cells with the antigenic and functional characteristics of intrathymic thymocytes. These changes included the acquisition of the differentiation antigens TL and Thy-1 (theta) and the ability to respond to the mitogen Con-A. Thymopoietin appears to be highly speicfic in inducing the prothymocyte to be highly specific in inducing the prothymocyte to thymocyte differentiation and does not affect the pluripotential stem cell measured by the colony forming assay (CFU-S), the erythropoietin-sensitive cell or B-cells. Experiments are in progress to determine whether additional hormonal inductive signals are required to complete the differentiation of an immunologically competent T-cell.", "contents": "Antigenic and functional evidence for the in vitro inductive activity of thymopoietin (thymin) on thymocyte precursors. Thymopoietin, a polypeptide hormone isolated from bovine thymus, induced in vitro the differentiation of prothymocytes to cells with the antigenic and functional characteristics of intrathymic thymocytes. These changes included the acquisition of the differentiation antigens TL and Thy-1 (theta) and the ability to respond to the mitogen Con-A. Thymopoietin appears to be highly speicfic in inducing the prothymocyte to be highly specific in inducing the prothymocyte to thymocyte differentiation and does not affect the pluripotential stem cell measured by the colony forming assay (CFU-S), the erythropoietin-sensitive cell or B-cells. Experiments are in progress to determine whether additional hormonal inductive signals are required to complete the differentiation of an immunologically competent T-cell."}
{"id": "PMID:1093472", "title": "Involvement of T- and B-lymphocytes in the immune response to the protein exotoxin and the lipopolysaccharide antigens of Vibrio cholerae.", "content": "The immune response at the level of individual immunocytes to the somatic lipopolysaccharide antigen derived from whole Vibrio cholerae and to the purified protein exotoxin from this organism were studied in terms of the role of T- and B-lymphocytes. By adoptive cell transfer studies with irradiated recipient mice, it was shown that normal spleen cells from normal syngeneic mice could readily transfer the capability of responding to both types of cholera antigens. However, when the spleen cells were depleted of T-cells with anti-theta serum and complement, antibody responsiveness to the LPS antigen, but not to exotoxin, could be achieved in recipients. Furthermore, by appropriate transfer of either bone marrow, thymus, or thymus-marrow cell mixtures to irradiated mice, it was shown that the response to the cholera somatic antigen was relatively independent of thymus cells, whereas the response to exotoxin required \"helper\" T-cells. The role of thymus and bone marrow cells in the intestinal tract in immune responses to the somatic and toxic antigens of cholera vibrios requires further investigation. Further studies should also provide additional information not only concerning the mechanism of the immune response to these antigens in terms of basic mechanisms of antibody formation, but also should provide valuable information in terms of anticholera immunity per se.", "contents": "Involvement of T- and B-lymphocytes in the immune response to the protein exotoxin and the lipopolysaccharide antigens of Vibrio cholerae. The immune response at the level of individual immunocytes to the somatic lipopolysaccharide antigen derived from whole Vibrio cholerae and to the purified protein exotoxin from this organism were studied in terms of the role of T- and B-lymphocytes. By adoptive cell transfer studies with irradiated recipient mice, it was shown that normal spleen cells from normal syngeneic mice could readily transfer the capability of responding to both types of cholera antigens. However, when the spleen cells were depleted of T-cells with anti-theta serum and complement, antibody responsiveness to the LPS antigen, but not to exotoxin, could be achieved in recipients. Furthermore, by appropriate transfer of either bone marrow, thymus, or thymus-marrow cell mixtures to irradiated mice, it was shown that the response to the cholera somatic antigen was relatively independent of thymus cells, whereas the response to exotoxin required \"helper\" T-cells. The role of thymus and bone marrow cells in the intestinal tract in immune responses to the somatic and toxic antigens of cholera vibrios requires further investigation. Further studies should also provide additional information not only concerning the mechanism of the immune response to these antigens in terms of basic mechanisms of antibody formation, but also should provide valuable information in terms of anticholera immunity per se."}
{"id": "PMID:1093473", "title": "The absolute requirement for T-cells in the induction of IgM-secreting cells, in vitro.", "content": "Anti-rabbit thymocyte antibody can totally inhibit the induction of IgM production that ordinarily is observed whem lymphoid cells are incubated, in virto, in the absence of added antigen. Univalent as well as bivalent antithymocyte antibody preparations were inhibitory when added to cells before the induction of immunoglobulin production had occurred but not afterwards. Spleen cells that had been treated with antithymocyte antibody and then cultured with thymocytes for 72 hours exhibited an enhanced inducttion of immunoglobulin production. Untreated spleen cells also showed this property, although both untreated lymph node cells and lymph node cells treated with antithymocyte antiboyd did not respond to thymocytes. The enhancement of the induction of immunoglobulin production by lipopolysaccharide was found to be T-cell dependent as judged from studies using antithymocyte antibody.", "contents": "The absolute requirement for T-cells in the induction of IgM-secreting cells, in vitro. Anti-rabbit thymocyte antibody can totally inhibit the induction of IgM production that ordinarily is observed whem lymphoid cells are incubated, in virto, in the absence of added antigen. Univalent as well as bivalent antithymocyte antibody preparations were inhibitory when added to cells before the induction of immunoglobulin production had occurred but not afterwards. Spleen cells that had been treated with antithymocyte antibody and then cultured with thymocytes for 72 hours exhibited an enhanced inducttion of immunoglobulin production. Untreated spleen cells also showed this property, although both untreated lymph node cells and lymph node cells treated with antithymocyte antiboyd did not respond to thymocytes. The enhancement of the induction of immunoglobulin production by lipopolysaccharide was found to be T-cell dependent as judged from studies using antithymocyte antibody."}
{"id": "PMID:1093475", "title": "The nature and function of T-cell antigens.", "content": "T-lymphocytes differ antigenically from B-lymphocytes. In the present study attempts were made to determine the role of surface antigens of T-cells, in their migration in vivo and in their response to mitogens. Exposure of thymus cells to anti-H2 sera inhibits migration to the lymph nodes (LN) to a greater extent than to the spleen. Fab fragments of H-2 antisera had only a slight effect on lymphocyte migration, inhibiting the LN-seeking stream only slightly more than the spleen-seeking stream. The interaction of Con-A with carbohydrates on the cell-surface of lymphocytes inhibits preferentially their localization in LN. Studies on the migration of lymphocytes that had localized either in the LN or spleens of primary host indicate that Con-A does not eliminate LN-seeking cells, but rather inhibits their active localization in LN. The subpopulation of lymphocytes, in both thymus and spleen that responds to Con-A was found to possess a higher H-2 antigenicity and a lower Ly and theta-antigenicity than the cells responding to PHA. Spleen cells responding to low concentrations of PHA had a relatively high H-2 antigenicity, whereas thos responding to high concentrations of PHA had a low H-2 antigenicity. Exposure of thymus cells to H-2 antiserum alone markedly inhibited their response to Con-A. Similar treatment of spleen cells had only a weak inhibitory effect.", "contents": "The nature and function of T-cell antigens. T-lymphocytes differ antigenically from B-lymphocytes. In the present study attempts were made to determine the role of surface antigens of T-cells, in their migration in vivo and in their response to mitogens. Exposure of thymus cells to anti-H2 sera inhibits migration to the lymph nodes (LN) to a greater extent than to the spleen. Fab fragments of H-2 antisera had only a slight effect on lymphocyte migration, inhibiting the LN-seeking stream only slightly more than the spleen-seeking stream. The interaction of Con-A with carbohydrates on the cell-surface of lymphocytes inhibits preferentially their localization in LN. Studies on the migration of lymphocytes that had localized either in the LN or spleens of primary host indicate that Con-A does not eliminate LN-seeking cells, but rather inhibits their active localization in LN. The subpopulation of lymphocytes, in both thymus and spleen that responds to Con-A was found to possess a higher H-2 antigenicity and a lower Ly and theta-antigenicity than the cells responding to PHA. Spleen cells responding to low concentrations of PHA had a relatively high H-2 antigenicity, whereas thos responding to high concentrations of PHA had a low H-2 antigenicity. Exposure of thymus cells to H-2 antiserum alone markedly inhibited their response to Con-A. Similar treatment of spleen cells had only a weak inhibitory effect."}
{"id": "PMID:1093480", "title": "Allergic contact dermatitis to underwear elastic. Chemically transformed by laundry bleach.", "content": "Six subjects, allergic to the elastic in their undergarments, were patch test-negative to the rubber components and new elastic from the suppliers. However, washing the rubber with sodium hypochlorite gave the subjects allergic responses due to the effect of bleach on the rubber accelerator, zinc dibenzyldithiocarbamate (ZDC). To identify the allergen, ZDC was reacted with the sodium hypochlorite, and the resultant gum was extracted with diethyl ether. Eight compounds were identified in the reaction mixture by gas chromatography-mass spectrometry. The individual components were tested on volunteers after sensitization to the reaction mixture was produced in 14 of 25 volunteers. One component, N,N-dibenzylcarbamyl chloride produced an allergic response in each sensitized volunteer.", "contents": "Allergic contact dermatitis to underwear elastic. Chemically transformed by laundry bleach. Six subjects, allergic to the elastic in their undergarments, were patch test-negative to the rubber components and new elastic from the suppliers. However, washing the rubber with sodium hypochlorite gave the subjects allergic responses due to the effect of bleach on the rubber accelerator, zinc dibenzyldithiocarbamate (ZDC). To identify the allergen, ZDC was reacted with the sodium hypochlorite, and the resultant gum was extracted with diethyl ether. Eight compounds were identified in the reaction mixture by gas chromatography-mass spectrometry. The individual components were tested on volunteers after sensitization to the reaction mixture was produced in 14 of 25 volunteers. One component, N,N-dibenzylcarbamyl chloride produced an allergic response in each sensitized volunteer."}
{"id": "PMID:1093481", "title": "Miconazole therapy for endemic fungal disease.", "content": "Miconazole nitrate (2.0%) cream was evaluated in the treatment of endemic dermatophytosis in an overcrowded prison. The active drug was compared in a random double-blind study with its control vehicle in the treatment of 99 inmates who had moderate to severe symptomatic disease. Tinea cruris and tinea pedis caused by Trichophyton rubrum predominated, although Trichophyton mentagrophytes and Candida albicans were also isolated and identified. Prompt clinical clearing occurred in 75.5% of those treated with miconazole versus 12.0% of those treated with the vehicle alone. Results of mycological examination closely paralleled and confirmed these clinical results.", "contents": "Miconazole therapy for endemic fungal disease. Miconazole nitrate (2.0%) cream was evaluated in the treatment of endemic dermatophytosis in an overcrowded prison. The active drug was compared in a random double-blind study with its control vehicle in the treatment of 99 inmates who had moderate to severe symptomatic disease. Tinea cruris and tinea pedis caused by Trichophyton rubrum predominated, although Trichophyton mentagrophytes and Candida albicans were also isolated and identified. Prompt clinical clearing occurred in 75.5% of those treated with miconazole versus 12.0% of those treated with the vehicle alone. Results of mycological examination closely paralleled and confirmed these clinical results."}
{"id": "PMID:1093482", "title": "Unilateral nevoid telangiectasia with gastric involvement.", "content": "A 49-year-old man with liver disease developed a unilateral telangiectatic lesion over the left trigeminal and left C3, C4, C5, and T1 dermatomal areas. This case fulfills the criteria for unilateral nervoid telangiectatic syndrome (UNTS), and as such represents the 11th reported case of UNTS and the first case, to our knowledge, of visceral involvement associated with a similar vascular malformation. A hemorrhagic tendency is present in this syndrome when there is gastrointestinal involvement.", "contents": "Unilateral nevoid telangiectasia with gastric involvement. A 49-year-old man with liver disease developed a unilateral telangiectatic lesion over the left trigeminal and left C3, C4, C5, and T1 dermatomal areas. This case fulfills the criteria for unilateral nervoid telangiectatic syndrome (UNTS), and as such represents the 11th reported case of UNTS and the first case, to our knowledge, of visceral involvement associated with a similar vascular malformation. A hemorrhagic tendency is present in this syndrome when there is gastrointestinal involvement."}
{"id": "PMID:1093483", "title": "Monoparesis. Complication of constant positive airways pressure.", "content": "Erb's palsy has been observed in 2 infants who had been treated with constant positive airways pressure for idiopathic respiratory distress syndrome. Both infants made complete neurological recovery from what was thought to be an acquired injury from the neck seal.", "contents": "Monoparesis. Complication of constant positive airways pressure. Erb's palsy has been observed in 2 infants who had been treated with constant positive airways pressure for idiopathic respiratory distress syndrome. Both infants made complete neurological recovery from what was thought to be an acquired injury from the neck seal."}
{"id": "PMID:1093485", "title": "Arterial stenosis complicating renal allotransplantation in man: a study of 38 cases.", "content": "Of 306 renal transplantations, stenosis of the artery supplying the grafted kidney was found in 38 patients three months to two years after they had undergone renal transplantation. The diagnosis was made by arteriography done because of refractory hypertension with or without impaired renal function in 36 patients and as a routine investigation in two normotensive patients. The stenosis was corrected surgically in 14 patients, with resultant lasting relief of hypertension in ten patients and improvement of renal function in five out of six patients with impaired renal function. Different types of stenosis were recognized: stenosis of the recipient artery, stenosis of the suture line, stenosis of the donor renal artery (segmental or diffuse) and multiple forms. The most frequent site of stenosis was the donor artery. There seems to be no single cause of stenosis: atheroma of the recipient vessels, faulty suture technique, hemodynamic disturbances, trauma to donor or recipient arteries account for some cases, whereas in other cases the evidence points to an immune mechanism. This complication of renal transplantation may be more frequent than is thought at present; therefore, routine renal arteriography should be performed at repeated intervals in all transplanted patients.", "contents": "Arterial stenosis complicating renal allotransplantation in man: a study of 38 cases. Of 306 renal transplantations, stenosis of the artery supplying the grafted kidney was found in 38 patients three months to two years after they had undergone renal transplantation. The diagnosis was made by arteriography done because of refractory hypertension with or without impaired renal function in 36 patients and as a routine investigation in two normotensive patients. The stenosis was corrected surgically in 14 patients, with resultant lasting relief of hypertension in ten patients and improvement of renal function in five out of six patients with impaired renal function. Different types of stenosis were recognized: stenosis of the recipient artery, stenosis of the suture line, stenosis of the donor renal artery (segmental or diffuse) and multiple forms. The most frequent site of stenosis was the donor artery. There seems to be no single cause of stenosis: atheroma of the recipient vessels, faulty suture technique, hemodynamic disturbances, trauma to donor or recipient arteries account for some cases, whereas in other cases the evidence points to an immune mechanism. This complication of renal transplantation may be more frequent than is thought at present; therefore, routine renal arteriography should be performed at repeated intervals in all transplanted patients."}
{"id": "PMID:1093486", "title": "Spontaneous reversal of portal blood flow: the case for and against its occurrence in patients with cirrhosis of the liver.", "content": "Because of its presumed serious clinical significance, we made an analysis of the evidence for and against the occurrence of spontaneous reversal of portal flow in cirrhosis of the liver. We examined the evidence obtained from manometric studies, radioactive tracer studies, radiologic studies, and actual measurements of magnitude and direction of portal blood flow. Concerning manometric studies, we introduced a physical analysis, based on first principles, which demonstrates that the occluded portal pressures cannot be used to construct a hydraulic gradient for portal flow. Similarly, we examined the weakness of the evidence derived from radioactive tracer and radiologic studies and, in the latter, the drastically opposite results reported by different investigators. Finally, we found that actual measurements of magnitude and direction of portal flow provide impressive evidence against the occurrence of spontaneous reversal of portal flow in cirrhosis. We conclude that unless new and convincing evidence is provided, it may not serve the best interests of medicine and of our patients to continue accepting spontaneous reversal of portal flow in cirrhosis as if it were a proven phenomenon.", "contents": "Spontaneous reversal of portal blood flow: the case for and against its occurrence in patients with cirrhosis of the liver. Because of its presumed serious clinical significance, we made an analysis of the evidence for and against the occurrence of spontaneous reversal of portal flow in cirrhosis of the liver. We examined the evidence obtained from manometric studies, radioactive tracer studies, radiologic studies, and actual measurements of magnitude and direction of portal blood flow. Concerning manometric studies, we introduced a physical analysis, based on first principles, which demonstrates that the occluded portal pressures cannot be used to construct a hydraulic gradient for portal flow. Similarly, we examined the weakness of the evidence derived from radioactive tracer and radiologic studies and, in the latter, the drastically opposite results reported by different investigators. Finally, we found that actual measurements of magnitude and direction of portal flow provide impressive evidence against the occurrence of spontaneous reversal of portal flow in cirrhosis. We conclude that unless new and convincing evidence is provided, it may not serve the best interests of medicine and of our patients to continue accepting spontaneous reversal of portal flow in cirrhosis as if it were a proven phenomenon."}
{"id": "PMID:1093487", "title": "Suppression of leukocyte chemotaxis in vitro by chemotherapeutic agents used in the management of thermal injuries.", "content": "Polymorphonuclear leukocytes from burned patients exhibit suppressed chemotaxis possibly related to the susceptibility of such patients to opportunistic infection. This study assesses the effect of normal serum upon burn-suppressed leukocytes and the effects of three commonly used topical chemotherapeutic agents upon the chemotaxis exhibited by granulocytes from normal controls. In vitro incubation with normal serum restored chemotaxis to normal in the suppressed granulocytes from burned patients. The serum factor responsible for this restoration was heat labile. Serum albumin alone did not exhibit this effect. Both mafenide and silver sulfadiazine suppressed the chemotactic function of granulocytes obtained from normal controls, while silver nitrate exhibited no such activity. Studies of the chemotactic function of control granulocytes after incubation with sera from burned patients yielded similar results; only the sera from patients treated with silver nitrate failed to suppress normal leukotaxis. The chemotactic impairment found in leukocytes from burned patients, however, while related to burn size and predictive of prognosis, did not vary with the agent used for the topical therapy. These data suggest the presence of a reversible intrinsic defect in leukotaxis consequent to burn injury, related to some factor deficient in burn serum. In addition, extrinsic impairment of normal granulocyte leukotaxis by two commonly used chemotherapeutic agents is demonstrated.", "contents": "Suppression of leukocyte chemotaxis in vitro by chemotherapeutic agents used in the management of thermal injuries. Polymorphonuclear leukocytes from burned patients exhibit suppressed chemotaxis possibly related to the susceptibility of such patients to opportunistic infection. This study assesses the effect of normal serum upon burn-suppressed leukocytes and the effects of three commonly used topical chemotherapeutic agents upon the chemotaxis exhibited by granulocytes from normal controls. In vitro incubation with normal serum restored chemotaxis to normal in the suppressed granulocytes from burned patients. The serum factor responsible for this restoration was heat labile. Serum albumin alone did not exhibit this effect. Both mafenide and silver sulfadiazine suppressed the chemotactic function of granulocytes obtained from normal controls, while silver nitrate exhibited no such activity. Studies of the chemotactic function of control granulocytes after incubation with sera from burned patients yielded similar results; only the sera from patients treated with silver nitrate failed to suppress normal leukotaxis. The chemotactic impairment found in leukocytes from burned patients, however, while related to burn size and predictive of prognosis, did not vary with the agent used for the topical therapy. These data suggest the presence of a reversible intrinsic defect in leukotaxis consequent to burn injury, related to some factor deficient in burn serum. In addition, extrinsic impairment of normal granulocyte leukotaxis by two commonly used chemotherapeutic agents is demonstrated."}
{"id": "PMID:1093489", "title": "Y-U advancement pyloroplasty.", "content": "Heineke in 1886 and von Mikulicz in 1887 independently described the popular method of pyloroplasty by longitudinal incision and transverse closure. In some patients, particularly children, the Heineke-Mikulicz procedure has not always been satisfactory because of inadequate size and distortion of the muscular gastroduodenal funnel. In 1958, Moschel and co-workers reintroduced the concept of the Y-V advancement principle to enlarge the pylorus. This operation has not been widely adopted but has a number of attractive features, including simplicity, safety, and maximal channel size with minimal operating time. The standard Y-V principle has been modified in favor of a broadly based U-shaped pedicle constructed from the presenting surface of the gastric antrum which is advanced into an incision made in the anterior duodenum. This operation has been used in 33 patients, who range in age from 3 weeks to 17 years. They have been variously followed from 6 months to 10 years. The surgical need for pyloroplasty in this group of patients included hiatal hernia with delayed gastric emptying (19 patients); vagotomy for ulcer (7 patients); esophageal substitution (4 patients); vagotomy for reflux esophagitis (2 patients); and, in one instance, revision of an obstructed Heineke-Mikulicz pyloroplasty. Complications have been minimal; gastric drainage has been highly satisfactory. In long-term followup, no ill effect has been seen from the presence of antral mucosa in the proximal duodenum.", "contents": "Y-U advancement pyloroplasty. Heineke in 1886 and von Mikulicz in 1887 independently described the popular method of pyloroplasty by longitudinal incision and transverse closure. In some patients, particularly children, the Heineke-Mikulicz procedure has not always been satisfactory because of inadequate size and distortion of the muscular gastroduodenal funnel. In 1958, Moschel and co-workers reintroduced the concept of the Y-V advancement principle to enlarge the pylorus. This operation has not been widely adopted but has a number of attractive features, including simplicity, safety, and maximal channel size with minimal operating time. The standard Y-V principle has been modified in favor of a broadly based U-shaped pedicle constructed from the presenting surface of the gastric antrum which is advanced into an incision made in the anterior duodenum. This operation has been used in 33 patients, who range in age from 3 weeks to 17 years. They have been variously followed from 6 months to 10 years. The surgical need for pyloroplasty in this group of patients included hiatal hernia with delayed gastric emptying (19 patients); vagotomy for ulcer (7 patients); esophageal substitution (4 patients); vagotomy for reflux esophagitis (2 patients); and, in one instance, revision of an obstructed Heineke-Mikulicz pyloroplasty. Complications have been minimal; gastric drainage has been highly satisfactory. In long-term followup, no ill effect has been seen from the presence of antral mucosa in the proximal duodenum."}
{"id": "PMID:1093490", "title": "Use of radionuclide imaging in the early diagnosis and treatment of renal allograft rejection.", "content": "Data are presented on the clinical application of radionuclide imaging to evaluate changes in cadaver transplant function in the immediate postoperative period. The method uses orthoiodohippuric acid (hippuran) administered IV, with scintillation imaging, and curve analysis by a digital computer. An initial study is always obtained 24 hours after transplantation. Serial studies are then obtained, as needed, to interpret the clinical course. Selected cases are presented which illustrate the use of this protocol in various clinical settings. In the oliguric patient serial studies have been of particular value. They have identified ATN so that over-enthusiastic treatment for rejection could be avoided. They have also identified acute rejection complicating ATN so that high dose steroid therapy could be administered appropriately. In the non-oliguric patient they have frequently contributed to the early diagnosis of acute rejection, and they have been useful in monitoring the effect and duration of treatment for severe rejection crisis. It is concluded that radionuclide imaging studies, when carefully applied and interpreted, are a valuable adjunct to the management of patients in this complex clinical setting.", "contents": "Use of radionuclide imaging in the early diagnosis and treatment of renal allograft rejection. Data are presented on the clinical application of radionuclide imaging to evaluate changes in cadaver transplant function in the immediate postoperative period. The method uses orthoiodohippuric acid (hippuran) administered IV, with scintillation imaging, and curve analysis by a digital computer. An initial study is always obtained 24 hours after transplantation. Serial studies are then obtained, as needed, to interpret the clinical course. Selected cases are presented which illustrate the use of this protocol in various clinical settings. In the oliguric patient serial studies have been of particular value. They have identified ATN so that over-enthusiastic treatment for rejection could be avoided. They have also identified acute rejection complicating ATN so that high dose steroid therapy could be administered appropriately. In the non-oliguric patient they have frequently contributed to the early diagnosis of acute rejection, and they have been useful in monitoring the effect and duration of treatment for severe rejection crisis. It is concluded that radionuclide imaging studies, when carefully applied and interpreted, are a valuable adjunct to the management of patients in this complex clinical setting."}
{"id": "PMID:1093491", "title": "Hypertension due to renal artery stenosis in transplanted kidneys.", "content": "Hypertension appeared to be related to stenosis of the hypogastricrenal artery system in 5 patients among 153 recipients of renal allografts. Renin assay and arteriography were crucial in the comprehensive evaluation of patients whose hypertension was not clearly related to rejection or excessive sodium intake. Hypereninemia was persistent in 4 of the 5 patients. Stenoses of the transplant renal arteries in three patients were caused by extensive intimal plaque formation. In one patient, periarterial fibrosis caused reduction of flow; 180 degrees torsion of the anastomosis resulted in stenosis in the fifth patient. Surgical correction is difficult and may be facilitated by a transabdominal approach. Vein bypass is probably preferable to patch angioplasty for intimal lesions. Following operation, hypertension was ameliorated and function improved in all patients. Rejection, which has been suggested as one of the causes of intimal plaque formation, ultimately led to the loss of the transplant in one patient. Function is normal in two patients; two patients have evidence of chronic rejection. No effort should be spared to evaluate this special group of patients whose transplant function can predictably be prolonged by decisive surgical management.", "contents": "Hypertension due to renal artery stenosis in transplanted kidneys. Hypertension appeared to be related to stenosis of the hypogastricrenal artery system in 5 patients among 153 recipients of renal allografts. Renin assay and arteriography were crucial in the comprehensive evaluation of patients whose hypertension was not clearly related to rejection or excessive sodium intake. Hypereninemia was persistent in 4 of the 5 patients. Stenoses of the transplant renal arteries in three patients were caused by extensive intimal plaque formation. In one patient, periarterial fibrosis caused reduction of flow; 180 degrees torsion of the anastomosis resulted in stenosis in the fifth patient. Surgical correction is difficult and may be facilitated by a transabdominal approach. Vein bypass is probably preferable to patch angioplasty for intimal lesions. Following operation, hypertension was ameliorated and function improved in all patients. Rejection, which has been suggested as one of the causes of intimal plaque formation, ultimately led to the loss of the transplant in one patient. Function is normal in two patients; two patients have evidence of chronic rejection. No effort should be spared to evaluate this special group of patients whose transplant function can predictably be prolonged by decisive surgical management."}
{"id": "PMID:1093492", "title": "Pulmonary responses of unilateral positive end expiratory pressure (PEEP) on experimental fat embolism.", "content": "The role of positive end expiratory pressure (PEEP) was evaluated in preventing the deleterious mechanical respiratory effects of fatty acid pulmonary embolism. One group of animals had ventilation without PEEP, while the second group had PEEP of 10 cm H2O applied only to the right lung. In the right lung, PEEP slightly reduced the blood flow, increased the vascular resistance, but reduced intersititial edema and reduced the degree of shunting to almost normal. Hypoxemia was prevented in the right pulmonary venous system, but was prominent in the left. The hypoxemia and shunting in the left lung were comparable to the Group I animals without PEEP to either lung. These studies confirm the value of PEEP in the therapy of the pulmonary manifestations of fat embolism which are the lethal factors in the fatty embolism syndrome.", "contents": "Pulmonary responses of unilateral positive end expiratory pressure (PEEP) on experimental fat embolism. The role of positive end expiratory pressure (PEEP) was evaluated in preventing the deleterious mechanical respiratory effects of fatty acid pulmonary embolism. One group of animals had ventilation without PEEP, while the second group had PEEP of 10 cm H2O applied only to the right lung. In the right lung, PEEP slightly reduced the blood flow, increased the vascular resistance, but reduced intersititial edema and reduced the degree of shunting to almost normal. Hypoxemia was prevented in the right pulmonary venous system, but was prominent in the left. The hypoxemia and shunting in the left lung were comparable to the Group I animals without PEEP to either lung. These studies confirm the value of PEEP in the therapy of the pulmonary manifestations of fat embolism which are the lethal factors in the fatty embolism syndrome."}
{"id": "PMID:1093493", "title": "Fifty-two consecutive aortic valve replacements employing local deep hypothermia.", "content": "From August, 1972, to May, 1974, 52 consecutive aortic valves were replaced with the Bj\u00f6rk-Shiley tilting-disc prosthesis. The hospital mortality was zero. Patients ranged in age from 14 to 77 years, and 19 patients had simultaneous correction of associated cardiac lesions. Using techniques of low flow and local deep hypothermia, aortic valve replacement can be accomplished with little risk even in patients with associated coronary arteriosclerosis. Coronary artery perfusion is unnecessary and often hazardous. Follow-up periods vary from one to twenty-one months. Forty-nine patients are alive and substantially improved.", "contents": "Fifty-two consecutive aortic valve replacements employing local deep hypothermia. From August, 1972, to May, 1974, 52 consecutive aortic valves were replaced with the Bj\u00f6rk-Shiley tilting-disc prosthesis. The hospital mortality was zero. Patients ranged in age from 14 to 77 years, and 19 patients had simultaneous correction of associated cardiac lesions. Using techniques of low flow and local deep hypothermia, aortic valve replacement can be accomplished with little risk even in patients with associated coronary arteriosclerosis. Coronary artery perfusion is unnecessary and often hazardous. Follow-up periods vary from one to twenty-one months. Forty-nine patients are alive and substantially improved."}
{"id": "PMID:1093494", "title": "Outbreaks of Serratia marcescens infections in a cardiothoracic surgical intensive care unit.", "content": "An outbreak of infections with pigmented Serratia marcescens involving 3 patients in a cardiothoracic surgical intensive care unit is reported. A respirator is thought to have been the source of pneumonia in 2 patients, and fomite spread from 1 of these is considered responsible for the induction of fatal endocarditis in the third patient. This outbreak demonstrates the rapid dissemination of a bacterial strain within the unit, several methods of dissemination, the wide variation in apparent virulence of the organism, the alterations of antibacterial host defense which made bacterial disease possible and which determined the site of infection, and the difficulties of adequate therapy. The third patient is the seventh reported with serratia infection of a prosthetic heart valve.", "contents": "Outbreaks of Serratia marcescens infections in a cardiothoracic surgical intensive care unit. An outbreak of infections with pigmented Serratia marcescens involving 3 patients in a cardiothoracic surgical intensive care unit is reported. A respirator is thought to have been the source of pneumonia in 2 patients, and fomite spread from 1 of these is considered responsible for the induction of fatal endocarditis in the third patient. This outbreak demonstrates the rapid dissemination of a bacterial strain within the unit, several methods of dissemination, the wide variation in apparent virulence of the organism, the alterations of antibacterial host defense which made bacterial disease possible and which determined the site of infection, and the difficulties of adequate therapy. The third patient is the seventh reported with serratia infection of a prosthetic heart valve."}
{"id": "PMID:1093495", "title": "Comparative hemodynamic consequences of inflation hold, PEEP, and interrupted PEEP: an experimental study in normal dogs.", "content": "Cardiac output and airway, intrathoracic, arterial, pulmonary artery, left atrial, and central venous pressures were studied in 8 mongrel dogs. They were anesthetized and ventilated with (1) inflation hold of various duration, (2) continuous positive end-expiratory pressure and one breath off). The results indicate a minimal decrease in cardiac output (of approximately 5 per cent) with inflation hold. Diminished cardiac output was noted with increasing levels of continuous PEEP. The severity of the decrease in cardiac output wasproportional to the airway pressure. The higher levels (20 cm H2O) OF PEEP were associated with profound decreases. Utilization of a technique of interrupted PEEP substantially reduced the adverse hemodynamic effects in the dog.", "contents": "Comparative hemodynamic consequences of inflation hold, PEEP, and interrupted PEEP: an experimental study in normal dogs. Cardiac output and airway, intrathoracic, arterial, pulmonary artery, left atrial, and central venous pressures were studied in 8 mongrel dogs. They were anesthetized and ventilated with (1) inflation hold of various duration, (2) continuous positive end-expiratory pressure and one breath off). The results indicate a minimal decrease in cardiac output (of approximately 5 per cent) with inflation hold. Diminished cardiac output was noted with increasing levels of continuous PEEP. The severity of the decrease in cardiac output wasproportional to the airway pressure. The higher levels (20 cm H2O) OF PEEP were associated with profound decreases. Utilization of a technique of interrupted PEEP substantially reduced the adverse hemodynamic effects in the dog."}
{"id": "PMID:1093496", "title": "Straight suture plane to avoid periprosthetic leak in aortic valve replacement.", "content": "A suture technique which avoids periprosthetic leakage during replacement of the aortic valve is described. Three types of stitch are inserted in a manner that causes all needle points to exit in a straight plane. The method was employed in 106 consecutive patients; of the 99 who survived operation, none have developed perivalvular leak.", "contents": "Straight suture plane to avoid periprosthetic leak in aortic valve replacement. A suture technique which avoids periprosthetic leakage during replacement of the aortic valve is described. Three types of stitch are inserted in a manner that causes all needle points to exit in a straight plane. The method was employed in 106 consecutive patients; of the 99 who survived operation, none have developed perivalvular leak."}
{"id": "PMID:1093497", "title": "Opportunistic endocarditis following open-heart surgery.", "content": "The incidence of endocarditis following open-heart operations is declining, but the relative incidence of endocarditis by the opportunistic organisms, both bacterial and fungal, has increased. These \"opportunists\" require careful identification and sensitivity testing so that appropriate therapy can be planned. In cases of candida endocarditis, a positive precipitin test and elevated candida antibody titer may provide an earlier means of diagnosis than blood cultures. These patients should be treated promptly with adequate dosages of specific antimicrobial or antifungal agents. Reoperation should be considered early if there is evidence of valve dehiscence or embolic phenomena or if drug therapy fails to control the infection.", "contents": "Opportunistic endocarditis following open-heart surgery. The incidence of endocarditis following open-heart operations is declining, but the relative incidence of endocarditis by the opportunistic organisms, both bacterial and fungal, has increased. These \"opportunists\" require careful identification and sensitivity testing so that appropriate therapy can be planned. In cases of candida endocarditis, a positive precipitin test and elevated candida antibody titer may provide an earlier means of diagnosis than blood cultures. These patients should be treated promptly with adequate dosages of specific antimicrobial or antifungal agents. Reoperation should be considered early if there is evidence of valve dehiscence or embolic phenomena or if drug therapy fails to control the infection."}
{"id": "PMID:1093498", "title": "[Comparison of the antiangina pectoris action of 2 coronary vasodilators].", "content": "Twenty four patients were studied: 7 males and 17 females, with an average age of 56 plus and minus 6.93 years, with a main diagnosis of ischaemic heart disease and with an average of at least 7 angina attacks per week. The anti-angina effect of 2 drugs, dipridamol and pentaeritritol was compared using the random double blind technique on parallel groups which were comparable. Pentaertritol administered for 4 weeks reduced, with a statistical significance (P 0.01), the average number of angina attacks of the group, as well as with taking nitroglicerine tablets. The number of pain free days was increased from 1.5 per week to 5.5 in 7 days. The effort capacity of the patients increased (P 0.01), and also the degree of elevation of the ST segment during and post effort. The changes obtained in these same areas with dipiridamol had no statistical significance.", "contents": "[Comparison of the antiangina pectoris action of 2 coronary vasodilators]. Twenty four patients were studied: 7 males and 17 females, with an average age of 56 plus and minus 6.93 years, with a main diagnosis of ischaemic heart disease and with an average of at least 7 angina attacks per week. The anti-angina effect of 2 drugs, dipridamol and pentaeritritol was compared using the random double blind technique on parallel groups which were comparable. Pentaertritol administered for 4 weeks reduced, with a statistical significance (P 0.01), the average number of angina attacks of the group, as well as with taking nitroglicerine tablets. The number of pain free days was increased from 1.5 per week to 5.5 in 7 days. The effort capacity of the patients increased (P 0.01), and also the degree of elevation of the ST segment during and post effort. The changes obtained in these same areas with dipiridamol had no statistical significance."}
{"id": "PMID:1093503", "title": "Cyclophosphamide therapy for rheumatoid arthritis.", "content": "Cyclophosphamide in high doses was given for six months to 19 patients with rheumatoid arthritis. A second group of patients with rheumatoid arthritis whose conditions were stable on low-dose prednisone received in addition either cyclophosphamide or placebo for six months. Measurements of joint function and joint inflammation were used to estimate disease activity. Joint inflammation progressively decreased and joint function improved in the high-dose group. The low-dose cyclophosphamide-plus-prednisone group had a similar response that was different from the prednisone-plus-placebo group. Cyclophosphamide toxicity was common in the high-dose group and minimal in the low-dose-plus-prednisone group. Cyclophosphamide therapy improved the arthritis of these patients. The results were almost as good in the low-dose-plus-prednisone group, and the toxicity was much less.", "contents": "Cyclophosphamide therapy for rheumatoid arthritis. Cyclophosphamide in high doses was given for six months to 19 patients with rheumatoid arthritis. A second group of patients with rheumatoid arthritis whose conditions were stable on low-dose prednisone received in addition either cyclophosphamide or placebo for six months. Measurements of joint function and joint inflammation were used to estimate disease activity. Joint inflammation progressively decreased and joint function improved in the high-dose group. The low-dose cyclophosphamide-plus-prednisone group had a similar response that was different from the prednisone-plus-placebo group. Cyclophosphamide toxicity was common in the high-dose group and minimal in the low-dose-plus-prednisone group. Cyclophosphamide therapy improved the arthritis of these patients. The results were almost as good in the low-dose-plus-prednisone group, and the toxicity was much less."}
{"id": "PMID:1093504", "title": "Transient bacteremia associated with barium enema.", "content": "A group of 175 patients had barium enema. Pour-plate blood cultures were obtained immediately before and after the procedure and 5, 10, 15, and 30 minutes later. Bacteremia was demonstrable in 20 (11.4%) patients. In some, blood cultures were positive for as long as 15 minutes after barium enema; all were negative at 30 minutes. Among the bacteria associated with the 20 episodes of bacteremia were Escherichia coli, Klebsiella, enterococci, Proteus morganii, Bacteroides, and Veillonella. The incidence of bacteremia among patients with ulcerative colitis, regional enteritis, rectal polyps, colonic or rectal carcinoma, nonspecific diarrhea, or other lower intestinal tract disorders was not much different from patients free of rectosigmoid disease. The results of this study suggest that a history of recent barium enema may be important in patients who have endocarditis.", "contents": "Transient bacteremia associated with barium enema. A group of 175 patients had barium enema. Pour-plate blood cultures were obtained immediately before and after the procedure and 5, 10, 15, and 30 minutes later. Bacteremia was demonstrable in 20 (11.4%) patients. In some, blood cultures were positive for as long as 15 minutes after barium enema; all were negative at 30 minutes. Among the bacteria associated with the 20 episodes of bacteremia were Escherichia coli, Klebsiella, enterococci, Proteus morganii, Bacteroides, and Veillonella. The incidence of bacteremia among patients with ulcerative colitis, regional enteritis, rectal polyps, colonic or rectal carcinoma, nonspecific diarrhea, or other lower intestinal tract disorders was not much different from patients free of rectosigmoid disease. The results of this study suggest that a history of recent barium enema may be important in patients who have endocarditis."}
{"id": "PMID:1093505", "title": "Anticoagulants in cerebrovascular disease. A critical review of studies.", "content": "Anticoagulant therapy in the treatment of cerebral thromboembolism remains controversial despite 20 years of statistical studies. Among the unresolved questions are (1) the relative value of aspirin vs coumarin derivatives for long-term therapy; (2) the relative benefits of long-term therapy vs short-term therapy; (3) the complications of anticoagulant therapy vs their therapeutic benefits; and (4) the optimal therapy of the various types of cerebral thromboembolic events, eg, transient ischemic attacks, strokes in evolution, and completed strokes. Much of this controversy derives from the same problems that have plagued the question of anticoagulation for acute myocardial infarction, namely that \"the existing statistics were obtained without adequate attention to fundamental principles of clinical science\". We have examined the available clinical studies bearing on this issue and are reporting our analysis of these studies.", "contents": "Anticoagulants in cerebrovascular disease. A critical review of studies. Anticoagulant therapy in the treatment of cerebral thromboembolism remains controversial despite 20 years of statistical studies. Among the unresolved questions are (1) the relative value of aspirin vs coumarin derivatives for long-term therapy; (2) the relative benefits of long-term therapy vs short-term therapy; (3) the complications of anticoagulant therapy vs their therapeutic benefits; and (4) the optimal therapy of the various types of cerebral thromboembolic events, eg, transient ischemic attacks, strokes in evolution, and completed strokes. Much of this controversy derives from the same problems that have plagued the question of anticoagulation for acute myocardial infarction, namely that \"the existing statistics were obtained without adequate attention to fundamental principles of clinical science\". We have examined the available clinical studies bearing on this issue and are reporting our analysis of these studies."}
{"id": "PMID:1093506", "title": "Methylphenidate and haloperidol in children. Effects on attention, memory, and activity.", "content": "Twenty-four hyperactive or unsocialized-aggressive children participated in an exploratory double-blind crossover study comparing the cognitive effects of methylphenidate hydrochloride (0.3 mg/kg), two doses (0.25 and 0.05 mg/kg) of haloperidol, and inert placebo. Each subject received each of four drug conditions, drug order being randomized by a Latin square design. At the end of each 18-day trial period, attention, immediate recognition memory, reaction times, and seat activity were tested. While drug effects were small, methylphenidate and, to a lesser extent, the low dose of haloperidol, facilitated performance, whereas there was some suggestion that the high dose of haloperidol may have caused a slight deterioration in performance. These results are contrasted with effects on social behavior where the latter improved behavior. No differences attributable to diagnosis were found.", "contents": "Methylphenidate and haloperidol in children. Effects on attention, memory, and activity. Twenty-four hyperactive or unsocialized-aggressive children participated in an exploratory double-blind crossover study comparing the cognitive effects of methylphenidate hydrochloride (0.3 mg/kg), two doses (0.25 and 0.05 mg/kg) of haloperidol, and inert placebo. Each subject received each of four drug conditions, drug order being randomized by a Latin square design. At the end of each 18-day trial period, attention, immediate recognition memory, reaction times, and seat activity were tested. While drug effects were small, methylphenidate and, to a lesser extent, the low dose of haloperidol, facilitated performance, whereas there was some suggestion that the high dose of haloperidol may have caused a slight deterioration in performance. These results are contrasted with effects on social behavior where the latter improved behavior. No differences attributable to diagnosis were found."}
{"id": "PMID:1093507", "title": "Marihuana and setting.", "content": "Marihuana or placebo cigarettes were smoked by 12 subjects in two environments, one \"favorable\" and one \"neutral\". The object was to determine the contribution of setting to the effects reported from the drug. Two quantifiable self-report measurements, the linear euphoriant scale and the card-sort version of the Addiction Research Center Inventory (marihuana and hallucinogen scales), were the major reporting criteria. Analyses of variance consistently demonstrated strong effects for subjects and drug but not for the environmental conditions. Reports of marihuana effects may be assumed to be highly colored by psychological differences in the mental set of subjects, or biological variations in their responses to the drug. The actual environment in which the drug is taken seems to play little, if any, role.", "contents": "Marihuana and setting. Marihuana or placebo cigarettes were smoked by 12 subjects in two environments, one \"favorable\" and one \"neutral\". The object was to determine the contribution of setting to the effects reported from the drug. Two quantifiable self-report measurements, the linear euphoriant scale and the card-sort version of the Addiction Research Center Inventory (marihuana and hallucinogen scales), were the major reporting criteria. Analyses of variance consistently demonstrated strong effects for subjects and drug but not for the environmental conditions. Reports of marihuana effects may be assumed to be highly colored by psychological differences in the mental set of subjects, or biological variations in their responses to the drug. The actual environment in which the drug is taken seems to play little, if any, role."}
{"id": "PMID:1093509", "title": "Etiology and treatment of homosexuality: a review.", "content": "The major causal theories of and treatment approaches to male and female homosexuality are critically reviewed. Neither biological, psychoanalytic, nor learning and social-learning theories are found to provide convincing evidence for the etiology of homosexuality. All of these accounts, however, are viewed as providing mixed empirical support for their predictions, with social-learning research presenting the most consistent evidence. It is argued that both social-learning research findings and results from retrospective studies suggest that homosexuality may best be linked to the early qualitative learning and development of one's gender identity and gender role. Both psychoanalytic therapy and behavior therapy are found to have minimal successes and many failures. Most therapeutic successes seem to be with bisexuals rather than with exclusive homosexuals. The combined use of psychotherapy and specific behavioral techniques is seen to offer some promise for heterosexual adaptation with certain kinds of patients. However, it is argued that better prospects for intervention in homosexuality lie in its prevention through the early identification and treatment of the potential homosexual child.", "contents": "Etiology and treatment of homosexuality: a review. The major causal theories of and treatment approaches to male and female homosexuality are critically reviewed. Neither biological, psychoanalytic, nor learning and social-learning theories are found to provide convincing evidence for the etiology of homosexuality. All of these accounts, however, are viewed as providing mixed empirical support for their predictions, with social-learning research presenting the most consistent evidence. It is argued that both social-learning research findings and results from retrospective studies suggest that homosexuality may best be linked to the early qualitative learning and development of one's gender identity and gender role. Both psychoanalytic therapy and behavior therapy are found to have minimal successes and many failures. Most therapeutic successes seem to be with bisexuals rather than with exclusive homosexuals. The combined use of psychotherapy and specific behavioral techniques is seen to offer some promise for heterosexual adaptation with certain kinds of patients. However, it is argued that better prospects for intervention in homosexuality lie in its prevention through the early identification and treatment of the potential homosexual child."}
{"id": "PMID:1093510", "title": "The relationship between paranoid delusions and homosexuality.", "content": "Freud predicted that paranoid delusions are motivated by unconscious homosexual impulses. The research conductedto test this hypothesis has been reviewed and it is concluded that the results do not support Freud's position. The results indicate anassociation between homosexual concerns and paranoid delusions but not in the manner that FREUD PREDICTED. For example, clinical studies indicate greater overt and conscious homosexual preoccupation in paranoid psychotics compared to nonparanoid psychotics, a finding not predicted by Freud. While psychological studies have tended to find more support for the Freudian position, several studies have failed to find supporting evidence and others are inconsistent in their results.", "contents": "The relationship between paranoid delusions and homosexuality. Freud predicted that paranoid delusions are motivated by unconscious homosexual impulses. The research conductedto test this hypothesis has been reviewed and it is concluded that the results do not support Freud's position. The results indicate anassociation between homosexual concerns and paranoid delusions but not in the manner that FREUD PREDICTED. For example, clinical studies indicate greater overt and conscious homosexual preoccupation in paranoid psychotics compared to nonparanoid psychotics, a finding not predicted by Freud. While psychological studies have tended to find more support for the Freudian position, several studies have failed to find supporting evidence and others are inconsistent in their results."}
{"id": "PMID:1093511", "title": "Rectovaginal separation operation after a \"cutback\" procedure for anorectal anomalies.", "content": "The \"cutback\" procedure has been widely utilized over the 25 years for correction of mild forms of ectopic anus encountered in newborn females. As these children have become adolescents, some are troubled by the cosmetic appearance created by this procedure, recurrent urinary tract infections, and persistent vaginitis from continued fecal soiling. The rectovaginal separation procedure described (1) separates the rectum from the vagina by interposing adjacent tissues, (2) elongates the rectal canal, and (3) utilizes flaps of perineal skin to gain a normal degree of separation of the anus from the vagina. Five girls, ages 6 to 15 years, have undergone this procedure with a temporary loop colostomy to protect the perineum during healing. The long-term functional and cosmetic results three to seven years later have been excellent in four patients. The fifth girl has been observed only five months, but seems to have an excellent cosmetic result.", "contents": "Rectovaginal separation operation after a \"cutback\" procedure for anorectal anomalies. The \"cutback\" procedure has been widely utilized over the 25 years for correction of mild forms of ectopic anus encountered in newborn females. As these children have become adolescents, some are troubled by the cosmetic appearance created by this procedure, recurrent urinary tract infections, and persistent vaginitis from continued fecal soiling. The rectovaginal separation procedure described (1) separates the rectum from the vagina by interposing adjacent tissues, (2) elongates the rectal canal, and (3) utilizes flaps of perineal skin to gain a normal degree of separation of the anus from the vagina. Five girls, ages 6 to 15 years, have undergone this procedure with a temporary loop colostomy to protect the perineum during healing. The long-term functional and cosmetic results three to seven years later have been excellent in four patients. The fifth girl has been observed only five months, but seems to have an excellent cosmetic result."}
{"id": "PMID:1093512", "title": "The solitary pulmonary nodule. Ten-year follow-up of veterans administration-armed forces cooperative study.", "content": "Over a five-year span 1,134 patients with asymptomatic solitary pulmonary nodules were entered into a cooperative study. Of the 392 lesions found to be primary bronchogenic carcinoma, 67 patients were living and under observation ten years following operation. In the study, 32% of lesions were primary bronchogenic carcinoma, the incidence being 51% in patients above the age of 50 years. \"Curative\" resection was possible in 309 patients (78.9%) with a five-year observed survival of 38.5% and a ten-year observed survival of 20.1%. Factors that influenced long-term survival were size of lesion, age at operation, and interval between the last normal and the first abnormal x-ray film. Histologic cell type and extent of resection were not found to influence long-term survival.", "contents": "The solitary pulmonary nodule. Ten-year follow-up of veterans administration-armed forces cooperative study. Over a five-year span 1,134 patients with asymptomatic solitary pulmonary nodules were entered into a cooperative study. Of the 392 lesions found to be primary bronchogenic carcinoma, 67 patients were living and under observation ten years following operation. In the study, 32% of lesions were primary bronchogenic carcinoma, the incidence being 51% in patients above the age of 50 years. \"Curative\" resection was possible in 309 patients (78.9%) with a five-year observed survival of 38.5% and a ten-year observed survival of 20.1%. Factors that influenced long-term survival were size of lesion, age at operation, and interval between the last normal and the first abnormal x-ray film. Histologic cell type and extent of resection were not found to influence long-term survival."}
{"id": "PMID:1093513", "title": "Changes in functional residual capacity of the lung after operation.", "content": "Functional residual capacity (FRC) of the lung was measured by the closed-circuit helium equilibration method before and for five days after upper abdominal operations in 28 patients (25 had cholecystectomies). Measurements in many were made with the patient both sitting in bed and sitting in a chair. Vital capacity (VC), residual volume (RV), and forced expiratory volume in one second (FEV1), as well as FRC, all decreased after operation, with the maximum decrease on days 1 and 2 and a gradual return toward preoperative values by day 5. Patients with 40% or less decrease in FRC after operation did not develop pulmonary complications. Change in position from bed to chair increased FRC 14.2% preoperatively and 17% postoperatively. The use of intermittent positive pressure breathing had no measurable effect on FRC. Similar changes in FEV1, VC, and RV also occurred, with maximum decreases on day 1.", "contents": "Changes in functional residual capacity of the lung after operation. Functional residual capacity (FRC) of the lung was measured by the closed-circuit helium equilibration method before and for five days after upper abdominal operations in 28 patients (25 had cholecystectomies). Measurements in many were made with the patient both sitting in bed and sitting in a chair. Vital capacity (VC), residual volume (RV), and forced expiratory volume in one second (FEV1), as well as FRC, all decreased after operation, with the maximum decrease on days 1 and 2 and a gradual return toward preoperative values by day 5. Patients with 40% or less decrease in FRC after operation did not develop pulmonary complications. Change in position from bed to chair increased FRC 14.2% preoperatively and 17% postoperatively. The use of intermittent positive pressure breathing had no measurable effect on FRC. Similar changes in FEV1, VC, and RV also occurred, with maximum decreases on day 1."}
{"id": "PMID:1093514", "title": "Hemorrhage due to diffuse erosive gastritis.", "content": "Of 796 patients admitted with upper gastrointestinal bleeding during a five-year period, 156 (20%) had erosive gastritis. Vigorous nonoperative treatment stopped bleeding in 117 (75%); emergency operation was required  in 39 (25%). In 24 of these 39 patients (group A), subtotal gastric resection with truncal vagotomy was performed. In the remaining 15 patients (group B), the operation was limited to pyloroplasty, truncal vagotomy, and multiple suture ligation. These patients were, on the average, 20 years older than the patients in group A, and 12 had life-threatening preoperative problems. (Only four of the group A patients had life-threatening preoperative problems.) The operative mortality in group A was 42%, with rebleeding occurring 33%. In group B, only 17% rebled and the operative mortality was 45%. Survival in the two groups was similar, even though those patients in group B were more critically ill. The less radical procedure may often be the best choice in the high-risk and elderly patients who bleed massively and in whom immediate salvage of life is the main issue.", "contents": "Hemorrhage due to diffuse erosive gastritis. Of 796 patients admitted with upper gastrointestinal bleeding during a five-year period, 156 (20%) had erosive gastritis. Vigorous nonoperative treatment stopped bleeding in 117 (75%); emergency operation was required  in 39 (25%). In 24 of these 39 patients (group A), subtotal gastric resection with truncal vagotomy was performed. In the remaining 15 patients (group B), the operation was limited to pyloroplasty, truncal vagotomy, and multiple suture ligation. These patients were, on the average, 20 years older than the patients in group A, and 12 had life-threatening preoperative problems. (Only four of the group A patients had life-threatening preoperative problems.) The operative mortality in group A was 42%, with rebleeding occurring 33%. In group B, only 17% rebled and the operative mortality was 45%. Survival in the two groups was similar, even though those patients in group B were more critically ill. The less radical procedure may often be the best choice in the high-risk and elderly patients who bleed massively and in whom immediate salvage of life is the main issue."}
{"id": "PMID:1093515", "title": "Dissecting aneurysm of the descending aorta. Improved surgical results in 91 patients.", "content": "Dissecting aneurysm of the descending thoraic aorta (type iii) is a specific disease process whose pathogenesis, pathologic description, surgical treatment, and prognosis is different from dissections of the ascending arota (types l and ll). From 1964 through 1974, 91 patients underwent surgical correction of type  iii dissection. The operative mortality was 21% and late mortality, up to ten years follow-up, was 13%. During the past four years, operative mortality has dropped to 6.5%. Mortality was determined by extent of the aneurysm and underlying cardiac disease. Antihypertensive and negative ionotropic drugs have not been used as definitive treatment but as adjuncts in perioperative support. Based on our present mortality of 6.5%, with no late mortality, we urge early surgical treatment of acute and chronic dissecting aneurysms of the descending thoracic aorta before extension, rupture, or massive enlargement of the aneurysm occurs.", "contents": "Dissecting aneurysm of the descending aorta. Improved surgical results in 91 patients. Dissecting aneurysm of the descending thoraic aorta (type iii) is a specific disease process whose pathogenesis, pathologic description, surgical treatment, and prognosis is different from dissections of the ascending arota (types l and ll). From 1964 through 1974, 91 patients underwent surgical correction of type  iii dissection. The operative mortality was 21% and late mortality, up to ten years follow-up, was 13%. During the past four years, operative mortality has dropped to 6.5%. Mortality was determined by extent of the aneurysm and underlying cardiac disease. Antihypertensive and negative ionotropic drugs have not been used as definitive treatment but as adjuncts in perioperative support. Based on our present mortality of 6.5%, with no late mortality, we urge early surgical treatment of acute and chronic dissecting aneurysms of the descending thoracic aorta before extension, rupture, or massive enlargement of the aneurysm occurs."}
{"id": "PMID:1093516", "title": "The permanent gastrostomy. Use of the gastrointestinal anastomotic stapler.", "content": "A permanent feeding gastrostomy was quickly and simply constructed in 29 patients with low operative risk using the gastrointestinal anastomotic stapler. The technique consisted of a simple application of this stapler on a carefully selected section of the stomach to form a Janeway type of gastrostomy. Activation of the device placed two double rows of staples and cut between them. This left the mucosa in approximation both in the tube and in the body of the stomach. A few additional Lembert sutures was all that was necessary. There were no suture line leaks nor other complications peculiar to the method.", "contents": "The permanent gastrostomy. Use of the gastrointestinal anastomotic stapler. A permanent feeding gastrostomy was quickly and simply constructed in 29 patients with low operative risk using the gastrointestinal anastomotic stapler. The technique consisted of a simple application of this stapler on a carefully selected section of the stomach to form a Janeway type of gastrostomy. Activation of the device placed two double rows of staples and cut between them. This left the mucosa in approximation both in the tube and in the body of the stomach. A few additional Lembert sutures was all that was necessary. There were no suture line leaks nor other complications peculiar to the method."}
{"id": "PMID:1093522", "title": "[Quantitative estimation of the lymphatic bed density of film preparations (of organ membranes) using the luxometry (photometry) method].", "content": "The essence of the luxmetry technique is that we can make a conclusion of the density of lymph capillaries per a unit of the serous or other membrane on the basis of the difference in illumination of the microscope field with a cleared preparation without injection of lymph vessels and the preparation where the lymphatic network is injected with the Gerota's mass absorbing a part of light. With the help of the above method of luxmetry we can obtain new data about local and developmental changes in the density of lymph capillaries of the abdomen, epicardium, submucous basement of the sigmoid colon and skin as well as in some functional (pregnancy) and pathological states.", "contents": "[Quantitative estimation of the lymphatic bed density of film preparations (of organ membranes) using the luxometry (photometry) method]. The essence of the luxmetry technique is that we can make a conclusion of the density of lymph capillaries per a unit of the serous or other membrane on the basis of the difference in illumination of the microscope field with a cleared preparation without injection of lymph vessels and the preparation where the lymphatic network is injected with the Gerota's mass absorbing a part of light. With the help of the above method of luxmetry we can obtain new data about local and developmental changes in the density of lymph capillaries of the abdomen, epicardium, submucous basement of the sigmoid colon and skin as well as in some functional (pregnancy) and pathological states."}
{"id": "PMID:1093525", "title": "[Ultrastructure of the skin in systemic scleroderma].", "content": "Biopsy specimens of the skin of the back of the forearm and hand in 20 patients with systemic scleroderma were studied using histological and electron microscopy methods.  There were revealed evidences of intensified neofibrillogenesis, changes in collagen structures of the derma (destruction and defects of packing of collagenous fibrills, variability of their thickness in a fibre) and in the microcirculatory bed.  In 18 out of 20 cases the nuclei of the connective-tissue cells and cells of the basal layer of the epidermis contained the so-called nuclear bodies, which are considered by a number of authors as an indirect evidence of a viral infection.  The process of an intensified neoformation of collagen fibrils considerably predominated over destructive processes in collagen structures.", "contents": "[Ultrastructure of the skin in systemic scleroderma]. Biopsy specimens of the skin of the back of the forearm and hand in 20 patients with systemic scleroderma were studied using histological and electron microscopy methods.  There were revealed evidences of intensified neofibrillogenesis, changes in collagen structures of the derma (destruction and defects of packing of collagenous fibrills, variability of their thickness in a fibre) and in the microcirculatory bed.  In 18 out of 20 cases the nuclei of the connective-tissue cells and cells of the basal layer of the epidermis contained the so-called nuclear bodies, which are considered by a number of authors as an indirect evidence of a viral infection.  The process of an intensified neoformation of collagen fibrils considerably predominated over destructive processes in collagen structures."}
{"id": "PMID:1093526", "title": "[Pathological anatomy of pneumonia in Mycoplasma pneumoniae infection in children].", "content": "A total of 43 section observations on acute respiratory viral diseases in children aged between 13 days to 13 years are described.  Using the direct and indirect fluorescent antibody test in 11 observations M. pneumoniae antigen was detected; in 4 observations this antigen was represented as monoantigen, and in 7 observations--in association with viruses of influenza, parainfluenza and adenovirus.  The characteristic features of mycoplasma pneumoniae were noted; very high blood filling of vessels of all calibres with the involvement of the system of microcirculation of the lungs, with phenomana of pronounced dilatation of the capillary net and with a tendency to thrombo-formation; diffuse lesions (dystrophic and inflammatory changes) of large and small bronchi and bronchioles; plasmocellular reaction in interstitia of the lungs, perbronchial follicles and bifurcated lymphatic nodes; the presence of M. pneumoniae antigen in histological sections of the lungs.  The author thinks it advisable to discard the term \"M. pneumoniae-pneumonia\" and replace it by \"M. pneumoniae-infection\" with predominent localization of the process in the lungs.", "contents": "[Pathological anatomy of pneumonia in Mycoplasma pneumoniae infection in children]. A total of 43 section observations on acute respiratory viral diseases in children aged between 13 days to 13 years are described.  Using the direct and indirect fluorescent antibody test in 11 observations M. pneumoniae antigen was detected; in 4 observations this antigen was represented as monoantigen, and in 7 observations--in association with viruses of influenza, parainfluenza and adenovirus.  The characteristic features of mycoplasma pneumoniae were noted; very high blood filling of vessels of all calibres with the involvement of the system of microcirculation of the lungs, with phenomana of pronounced dilatation of the capillary net and with a tendency to thrombo-formation; diffuse lesions (dystrophic and inflammatory changes) of large and small bronchi and bronchioles; plasmocellular reaction in interstitia of the lungs, perbronchial follicles and bifurcated lymphatic nodes; the presence of M. pneumoniae antigen in histological sections of the lungs.  The author thinks it advisable to discard the term \"M. pneumoniae-pneumonia\" and replace it by \"M. pneumoniae-infection\" with predominent localization of the process in the lungs."}
{"id": "PMID:1093528", "title": "Expandable silicone implants for scleral buckling. IV. Experiments with an explant for macular breaks.", "content": "A new expandable silicone rubber explant used to indent the sclera at the macula was tested experimentally in rabbits. The device consists of a Dacron-reinforced silicone rubber strip that has an expandable portion (or balloon) at its midpoint connected to a silicone filling tube. The explant was expanded with 0.03 to 0.06 ml of an antibiotic solution. Smooth, round buckles that exerted even indentation were produced. A decrease in height of 20% to 50% was observed ophthalmoscopically during six months of follow-up. The degree of buckling can be easily controlled during operation and may be modified postoperatively.", "contents": "Expandable silicone implants for scleral buckling. IV. Experiments with an explant for macular breaks. A new expandable silicone rubber explant used to indent the sclera at the macula was tested experimentally in rabbits. The device consists of a Dacron-reinforced silicone rubber strip that has an expandable portion (or balloon) at its midpoint connected to a silicone filling tube. The explant was expanded with 0.03 to 0.06 ml of an antibiotic solution. Smooth, round buckles that exerted even indentation were produced. A decrease in height of 20% to 50% was observed ophthalmoscopically during six months of follow-up. The degree of buckling can be easily controlled during operation and may be modified postoperatively."}
{"id": "PMID:1093531", "title": "Malignant lymphoma of the paranasal sinuses.", "content": "Sinus lymphoma is a rare but potentially radiocurable malignant neoplasm. The disease is isolated to the sinus and adjacent local tissues on initial presentation, and concurrent generalized lymphoma is unusual. With rare exception, the histologic pattern is that of a diffuse histiocytic non-Hodgkin lymphoma (reticulum cell sarcoma). Radiotherapy to the involved sinuses without prophylactic neck irradiation is the treatment of choice, and a survival rate of 50 percent to 70 percent can be expected.", "contents": "Malignant lymphoma of the paranasal sinuses. Sinus lymphoma is a rare but potentially radiocurable malignant neoplasm. The disease is isolated to the sinus and adjacent local tissues on initial presentation, and concurrent generalized lymphoma is unusual. With rare exception, the histologic pattern is that of a diffuse histiocytic non-Hodgkin lymphoma (reticulum cell sarcoma). Radiotherapy to the involved sinuses without prophylactic neck irradiation is the treatment of choice, and a survival rate of 50 percent to 70 percent can be expected."}
{"id": "PMID:1093532", "title": "Skin and homograft cartilage reconstruction.", "content": "Conservative vertical hemilaryngectomy poses the problem of relating the need to remove the tumor completely to the aim of reconstructing an adequate airway lined with epithelium. An attempt has has been made in experimental animals to overcome this problem using pedicled skin and homograft cartilage. The findings suggest that this is a feasible technique, although somewhat hampered by problems unique to the animals concerned. The use of skin pedicles provides adequate cover without shrinkage, and although it is doubtful whether cartilage can survive intact in the long-term, it would seem that it may be adequate for the purposes intended.", "contents": "Skin and homograft cartilage reconstruction. Conservative vertical hemilaryngectomy poses the problem of relating the need to remove the tumor completely to the aim of reconstructing an adequate airway lined with epithelium. An attempt has has been made in experimental animals to overcome this problem using pedicled skin and homograft cartilage. The findings suggest that this is a feasible technique, although somewhat hampered by problems unique to the animals concerned. The use of skin pedicles provides adequate cover without shrinkage, and although it is doubtful whether cartilage can survive intact in the long-term, it would seem that it may be adequate for the purposes intended."}
{"id": "PMID:1093533", "title": "Study of the adenovascular body of the posterior part of the nasal septum.", "content": "The mucosa of the posterior part of the nasal septum is studied by in vivo observations in 40 seemingly healthy adults, vascular injection of two anatomical specimens rendered transparent, histological studies of serial sections of five anatomical specimens, and macroscopic and histological examination of specimens obtained from 20 cadavers. The presence of an \"adenovascular\" body was noted in the posterior part of the nasal septum; this body occupies about one fifth of the total area of this region. This study shows that this is a normal structure of the nose and suggests that its name be changed to adenovascular body of the posterior part of the nasal septum.", "contents": "Study of the adenovascular body of the posterior part of the nasal septum. The mucosa of the posterior part of the nasal septum is studied by in vivo observations in 40 seemingly healthy adults, vascular injection of two anatomical specimens rendered transparent, histological studies of serial sections of five anatomical specimens, and macroscopic and histological examination of specimens obtained from 20 cadavers. The presence of an \"adenovascular\" body was noted in the posterior part of the nasal septum; this body occupies about one fifth of the total area of this region. This study shows that this is a normal structure of the nose and suggests that its name be changed to adenovascular body of the posterior part of the nasal septum."}
{"id": "PMID:1093534", "title": "Blood flow measurements in skin flaps.", "content": "For the last 50 years, the empirical approach of set length to width ratios for skin flaps has been held valid. Not until Milton's work in 1971 was this concept challenged. This report represents a method where precise, reproducible, and quantitative measurements of skin blood flow can be made using four radioactive nuclide microspheres and measures the blood flow changes in flaps of differing length to width ratios. Conclusions from our data suggest that the cutaneous blood flow in ventrally based, abdominal skin flaps in dogs is independent of base width as measured immediately after outlining and elevation.", "contents": "Blood flow measurements in skin flaps. For the last 50 years, the empirical approach of set length to width ratios for skin flaps has been held valid. Not until Milton's work in 1971 was this concept challenged. This report represents a method where precise, reproducible, and quantitative measurements of skin blood flow can be made using four radioactive nuclide microspheres and measures the blood flow changes in flaps of differing length to width ratios. Conclusions from our data suggest that the cutaneous blood flow in ventrally based, abdominal skin flaps in dogs is independent of base width as measured immediately after outlining and elevation."}
{"id": "PMID:1093535", "title": "Hearing loss during resuscitation.", "content": "Tympanic injury secondary to positive pressure mask ventillation is rare. During resuscitative procedures, two patients developed partial deafness, one from disarticulation of the incudomalleal joint, the other from an oval window perilymph fistula. Excessive pressure transmitted rapidly through the eustachian tube is postulated to have caused both injuries.", "contents": "Hearing loss during resuscitation. Tympanic injury secondary to positive pressure mask ventillation is rare. During resuscitative procedures, two patients developed partial deafness, one from disarticulation of the incudomalleal joint, the other from an oval window perilymph fistula. Excessive pressure transmitted rapidly through the eustachian tube is postulated to have caused both injuries."}
{"id": "PMID:1093537", "title": "Microorganism-like structures in Hodgkin disease. Electron microscopical demonstration.", "content": "Ultrastructural examination of lymph nodes from patients with untreated Hodgkin disease showed unique spherical structures that were found extracellularly and within vacuoles of reticulum cells, macrophages, mononuclear cells, and occasional tumor cells. These spheroids varied in size and internal composition possibly in relation to developmental stages, and resembled mycoplasma morphologically and in cellular distribution. The spheroids were found predominantly in the neoplastic areas in lymph nodes of four out of nine patients with Hodgkin disease of the mixed cell variety, but were not seen in other types of Hodgkin disease or reticulum cell sarcoma. The structures were much more numerous in the lymph nodes from young children with Hodgkin disease. A lymph node of one child, disclosed numerous extracellular structures resembling viral type C particles in addition to mycoplasma-like spheroids. The results indicate that subclinical infections may occur in Hodgkin disease and contribute to the histopathologic manifestations of the disease.", "contents": "Microorganism-like structures in Hodgkin disease. Electron microscopical demonstration. Ultrastructural examination of lymph nodes from patients with untreated Hodgkin disease showed unique spherical structures that were found extracellularly and within vacuoles of reticulum cells, macrophages, mononuclear cells, and occasional tumor cells. These spheroids varied in size and internal composition possibly in relation to developmental stages, and resembled mycoplasma morphologically and in cellular distribution. The spheroids were found predominantly in the neoplastic areas in lymph nodes of four out of nine patients with Hodgkin disease of the mixed cell variety, but were not seen in other types of Hodgkin disease or reticulum cell sarcoma. The structures were much more numerous in the lymph nodes from young children with Hodgkin disease. A lymph node of one child, disclosed numerous extracellular structures resembling viral type C particles in addition to mycoplasma-like spheroids. The results indicate that subclinical infections may occur in Hodgkin disease and contribute to the histopathologic manifestations of the disease."}
{"id": "PMID:1093538", "title": "Acute pulmonary alveolitis in narcotics abuse.", "content": "Lungs of 17 narcotic abusers from medical-legal autopsies were studied to determine the pathological basis for regional alterations of ventilation and perfusion. Postmortem pulmonary angiographic and histological techniques demonstrated acute alveolar wall inflammation with exudation of fluid and cells into the alveoli in all eight abusers dying of narcotism and in six of nine dying of other causes. All abusers had extensive focal chronic intersititial inflammatory infiltrates. Vascular obstruction due to foreign material occurred only with intravenous use of oral pharmaceuticals, and then only in the microcirculation. Lungs of ten young adults dying suddenly with no history or evidence of narcotics abuse showed no acute alveolitis or intravascular foreign material. Intravenous narcotics injection produces transient acute alveolar inflammation that may account for respiratory manifestations and death. Permanent injury to the lung parenchyma or larger vessels with the usual forms of narcotic abuse is not common.", "contents": "Acute pulmonary alveolitis in narcotics abuse. Lungs of 17 narcotic abusers from medical-legal autopsies were studied to determine the pathological basis for regional alterations of ventilation and perfusion. Postmortem pulmonary angiographic and histological techniques demonstrated acute alveolar wall inflammation with exudation of fluid and cells into the alveoli in all eight abusers dying of narcotism and in six of nine dying of other causes. All abusers had extensive focal chronic intersititial inflammatory infiltrates. Vascular obstruction due to foreign material occurred only with intravenous use of oral pharmaceuticals, and then only in the microcirculation. Lungs of ten young adults dying suddenly with no history or evidence of narcotics abuse showed no acute alveolitis or intravascular foreign material. Intravenous narcotics injection produces transient acute alveolar inflammation that may account for respiratory manifestations and death. Permanent injury to the lung parenchyma or larger vessels with the usual forms of narcotic abuse is not common."}
{"id": "PMID:1093539", "title": "Extracellular glomerular microparticles in nephrotic syndrome of heroin users.", "content": "Intracellular and extracellular glomerular microparticles (GMP) have been described in diverse human glomerulopathies. We have recently detected extracellular GMP in two heroin abusers with nephrotic syndrome. Most of the GMP were approximately 100 nm in diameter (range, 56 to 178 nm), and consisted ultrastructurally of an electron-dense central core surrounded by a concentric, lucent area and an electron-dense limiting membrane. Incomplete forms of GMP and residual laminated membranes without a central core were also present. The distribution of extracellular GMP was both intramembranous and subepithelial; they did not resemble virions morphologically. As these GMP have been observed in human glomerulopathies that are etiologically and morphologically varied, they probably represent nonspecific markers of glomerular injury. The prevalence of GMP in the nephrotic syndrome of heroin abusers awaits cumulative experience.", "contents": "Extracellular glomerular microparticles in nephrotic syndrome of heroin users. Intracellular and extracellular glomerular microparticles (GMP) have been described in diverse human glomerulopathies. We have recently detected extracellular GMP in two heroin abusers with nephrotic syndrome. Most of the GMP were approximately 100 nm in diameter (range, 56 to 178 nm), and consisted ultrastructurally of an electron-dense central core surrounded by a concentric, lucent area and an electron-dense limiting membrane. Incomplete forms of GMP and residual laminated membranes without a central core were also present. The distribution of extracellular GMP was both intramembranous and subepithelial; they did not resemble virions morphologically. As these GMP have been observed in human glomerulopathies that are etiologically and morphologically varied, they probably represent nonspecific markers of glomerular injury. The prevalence of GMP in the nephrotic syndrome of heroin abusers awaits cumulative experience."}
{"id": "PMID:1093540", "title": "The case of The Case of the Midwife Toad.", "content": "The facts concerning Kammerer's case are reviewed.  Koestler's view that Krammerer was unjustly accused is presented and shown to be incorrect.", "contents": "The case of The Case of the Midwife Toad. The facts concerning Kammerer's case are reviewed.  Koestler's view that Krammerer was unjustly accused is presented and shown to be incorrect."}
{"id": "PMID:1093541", "title": "Enzyme histochemical investigations of human malignant lymphomas.", "content": "26 cases of malignant lymphomas and 23 other lymphoreticular conditions were investigated enzyme histochemically. Each type of malignant lymphoma revealed a different enzyme histochemical pattern characteristic of its type. These features are not only applicable to differential diagnosis but also suggest clues to the understanding of histogenesis and nature of malignant lymphomas.", "contents": "Enzyme histochemical investigations of human malignant lymphomas. 26 cases of malignant lymphomas and 23 other lymphoreticular conditions were investigated enzyme histochemically. Each type of malignant lymphoma revealed a different enzyme histochemical pattern characteristic of its type. These features are not only applicable to differential diagnosis but also suggest clues to the understanding of histogenesis and nature of malignant lymphomas."}
{"id": "PMID:1093542", "title": "Gammacardiographic and ballistocardiographic comparison of indices of central haemodynamics and the myocardial contractile functional state in patients with coronary heart disease.", "content": "A complex ballistocardiographic and gammacardiographic investigation was carried out in 62 patients with coronary heart disease; ballistocardiographic and gammacardiographic indices have been compared. We established pathologic ballistocardiographic changes, which indicate a disturbance of the myocardial contractile function. Considerable changes of central haemodynamics, studied by means of gammacardiography, were recorded. There is a relationship between ballistocardiographic indices of the myocardial contractile function and radiocardiographic indices of central haemodynamics.", "contents": "Gammacardiographic and ballistocardiographic comparison of indices of central haemodynamics and the myocardial contractile functional state in patients with coronary heart disease. A complex ballistocardiographic and gammacardiographic investigation was carried out in 62 patients with coronary heart disease; ballistocardiographic and gammacardiographic indices have been compared. We established pathologic ballistocardiographic changes, which indicate a disturbance of the myocardial contractile function. Considerable changes of central haemodynamics, studied by means of gammacardiography, were recorded. There is a relationship between ballistocardiographic indices of the myocardial contractile function and radiocardiographic indices of central haemodynamics."}
{"id": "PMID:1093551", "title": "Effect of ibuprofen dosage on patient response in rheumatoid arthritis.", "content": "In a 4-week double-blind trial in 41 patients with rheumatoid arthritis, ibuprofen (Motrin) decreased the swollen joint count significantly at a dosage level of 2400 mg/day, but not at 1200 mg/day. Ibuprofen probably does have antiinflammatory effects in man, but only at higher dosages; antiarthritic activity at lower dosages reflects primarily analgesia and perhaps antipyretic effects. Complaints and clinical laboratory findings did not differ significantly between the dosage groups.", "contents": "Effect of ibuprofen dosage on patient response in rheumatoid arthritis. In a 4-week double-blind trial in 41 patients with rheumatoid arthritis, ibuprofen (Motrin) decreased the swollen joint count significantly at a dosage level of 2400 mg/day, but not at 1200 mg/day. Ibuprofen probably does have antiinflammatory effects in man, but only at higher dosages; antiarthritic activity at lower dosages reflects primarily analgesia and perhaps antipyretic effects. Complaints and clinical laboratory findings did not differ significantly between the dosage groups."}
{"id": "PMID:1093559", "title": "Effect of quinidine on maintaining sinus rhythm after conversion of atrial fibrillation or flutter. A multicentre study from Stockholm.", "content": "In a controlled study comprising 176 patients, quinidine in the form of Kinidin Durules was found to reduced significantly the recurrence of the atrial fibrillation during a 1-year follow-up period after successful electric shock conversion. After one year, 51 per cent (52/101) of the patients in the quinidine group, and 28 per cent (21/75) in the control group remained in sinus rhythm (P smaller than 0.001). No less than 43 per cent of the patients converted to sinus rhythm during treatment with maintenance doses of quinidine sulphate before intended DC conversion. Gastrointestinal side-effects were not uncommon, and caused interruption of quinidine treatment in some cases.", "contents": "Effect of quinidine on maintaining sinus rhythm after conversion of atrial fibrillation or flutter. A multicentre study from Stockholm. In a controlled study comprising 176 patients, quinidine in the form of Kinidin Durules was found to reduced significantly the recurrence of the atrial fibrillation during a 1-year follow-up period after successful electric shock conversion. After one year, 51 per cent (52/101) of the patients in the quinidine group, and 28 per cent (21/75) in the control group remained in sinus rhythm (P smaller than 0.001). No less than 43 per cent of the patients converted to sinus rhythm during treatment with maintenance doses of quinidine sulphate before intended DC conversion. Gastrointestinal side-effects were not uncommon, and caused interruption of quinidine treatment in some cases."}
{"id": "PMID:1093564", "title": "Recent progress in the study of occupational lung diseases in Romania.", "content": "This paper reviews studies of occupational lung diseases in Romania in the last two decades. Work concerned with the effects of exposure to textile fibres, irritant gases and fumes in the chemical industry, welding fumes, asbestos, cadmium oxide, and the relation between dust exposure, pneumoconiosis, and chronic bronchitis is briefly presented.", "contents": "Recent progress in the study of occupational lung diseases in Romania. This paper reviews studies of occupational lung diseases in Romania in the last two decades. Work concerned with the effects of exposure to textile fibres, irritant gases and fumes in the chemical industry, welding fumes, asbestos, cadmium oxide, and the relation between dust exposure, pneumoconiosis, and chronic bronchitis is briefly presented."}
{"id": "PMID:1093565", "title": "People and work: some contemporary issues.", "content": "In advanced industrial societies social, economic, and technological changes are accompanied by changing values and attitudes to work, symptomatic of what some see as the transition to a post-industrial era. As a result existing job definitions and traditional forms of organization are being challenged and attempts made to restructure work so that it becomes meaningful and rewarding in the fullest sense, to the individual, to the enterprise, and to society. These range from programmes of job enlargement and job enrichment, within the framework of existing technologies, to experiments in the design of organizations as a whole in which fewer constraints are accepted as given. They entail and require a multidisciplinary approach as well as awareness of and commitment to the underlying values. The possibilities and benefits of restructuring work in these various ways have been demonstrated sufficiently to encourage interest at governmental level as well as by employers and trade unions. There are, however, no simple prescriptions or principles of universal application. Knowledge is still tentative and partial but there is consensus that the search for new ways of dealing with the organization of work and the allocation of resources is of fundamental importance.", "contents": "People and work: some contemporary issues. In advanced industrial societies social, economic, and technological changes are accompanied by changing values and attitudes to work, symptomatic of what some see as the transition to a post-industrial era. As a result existing job definitions and traditional forms of organization are being challenged and attempts made to restructure work so that it becomes meaningful and rewarding in the fullest sense, to the individual, to the enterprise, and to society. These range from programmes of job enlargement and job enrichment, within the framework of existing technologies, to experiments in the design of organizations as a whole in which fewer constraints are accepted as given. They entail and require a multidisciplinary approach as well as awareness of and commitment to the underlying values. The possibilities and benefits of restructuring work in these various ways have been demonstrated sufficiently to encourage interest at governmental level as well as by employers and trade unions. There are, however, no simple prescriptions or principles of universal application. Knowledge is still tentative and partial but there is consensus that the search for new ways of dealing with the organization of work and the allocation of resources is of fundamental importance."}
{"id": "PMID:1093567", "title": "Cataract surgery performed before 800 B.C.", "content": "The general belief that the technique of cataract extraction in India in the Sushruta period (800 B.C.) was couching is no longer tenable. A study of the original text suggests that the method was more closely allied to the extracapsular extraction of recent times.", "contents": "Cataract surgery performed before 800 B.C. The general belief that the technique of cataract extraction in India in the Sushruta period (800 B.C.) was couching is no longer tenable. A study of the original text suggests that the method was more closely allied to the extracapsular extraction of recent times."}
{"id": "PMID:1093568", "title": "Chemical specificity of pyruvate kinase from yeast.", "content": "Three analogs of phosphoenolpyruvic acid: (Z)-phosphoenol-3-fluoropyruvate, (Z)-phosphoenol-3-bromopyruvate and (Z)-phosphoenol-alpha-ketobutyrate were found to be substrates for yeast pyruvate kinase (ATP: pyruvate (Z)-O-phosphotransferase, EC 2.7.1.40)with maximal velocities much greater than those found for rabbit muscle pyruvate kinase. The analogs exhibited sigmoidal kinetics, which become hyperbolic upon addition of the allosteric effector, fructose 1,6-diphosphate. Moreover, the reaction of (Z)-phosphoenol-3-bromopyruvate with ADP to produce bromopyruvic acid and ATP irreversibly inhibited the enzyme with a half-life of 32 min.", "contents": "Chemical specificity of pyruvate kinase from yeast. Three analogs of phosphoenolpyruvic acid: (Z)-phosphoenol-3-fluoropyruvate, (Z)-phosphoenol-3-bromopyruvate and (Z)-phosphoenol-alpha-ketobutyrate were found to be substrates for yeast pyruvate kinase (ATP: pyruvate (Z)-O-phosphotransferase, EC 2.7.1.40)with maximal velocities much greater than those found for rabbit muscle pyruvate kinase. The analogs exhibited sigmoidal kinetics, which become hyperbolic upon addition of the allosteric effector, fructose 1,6-diphosphate. Moreover, the reaction of (Z)-phosphoenol-3-bromopyruvate with ADP to produce bromopyruvic acid and ATP irreversibly inhibited the enzyme with a half-life of 32 min."}
{"id": "PMID:1093569", "title": "Stimulatory effect of hydrostatic pressure on cell division in cultures of Escherichia coli.", "content": "[14-C] Thymine incorporation into a trichloracetic acid-insoluble fraction of Escherichia coli HMS83 polA1 polB1 and cell division were observed as a function of time. The data presented show that hydrostatic pressures of about 600 x 10-5 N/m-2 stimulate that part of the division process which leads to the separation of cells. The kinetics of [14-C] thymine incorporation by this mutant suggest that completion of rounds of replication at high pressures is not dependent on DNA polymerases I or II or at least on high levels of these enzymes.", "contents": "Stimulatory effect of hydrostatic pressure on cell division in cultures of Escherichia coli. [14-C] Thymine incorporation into a trichloracetic acid-insoluble fraction of Escherichia coli HMS83 polA1 polB1 and cell division were observed as a function of time. The data presented show that hydrostatic pressures of about 600 x 10-5 N/m-2 stimulate that part of the division process which leads to the separation of cells. The kinetics of [14-C] thymine incorporation by this mutant suggest that completion of rounds of replication at high pressures is not dependent on DNA polymerases I or II or at least on high levels of these enzymes."}
{"id": "PMID:1093570", "title": "The effect of neomycin and streptomycin on the electrical polarisability of aqueous suspensions of Escherichia coli.", "content": "Aqueous suspensions of bacteria scatter light strongly. In addition, the bacteria exhibit strong induced dipole moments in an electric field. In this note we report how, by measuring the intensity of the scattered light, the electric polarisability (alpha) of Escherichia coli could be monitored as small quantities of antibiotics were added to the suspensions. The effect of the presence of quite small quantities of antibiotic on the electrical polarisability, which gave rise to the induced dipole, was dramatic. From the hypothesis that alpha had its origins in the bacteria-solvent interface, a theory is presented which adequately accounts for both alpha and its changes in the presence of these antibiotics. The study is taken to suggest that the antibiotic molecules were adsorbed on to the bacterial surface thereby reducing the surface charge. This in turn reduces the number of counterions and the apparent induced dipole moment. Because the electric-field scattering method is both quick and sensitive to changes in alpha, it may prove a valuable method for the study of antibiotic action on  cell and microorganism surfaces.", "contents": "The effect of neomycin and streptomycin on the electrical polarisability of aqueous suspensions of Escherichia coli. Aqueous suspensions of bacteria scatter light strongly. In addition, the bacteria exhibit strong induced dipole moments in an electric field. In this note we report how, by measuring the intensity of the scattered light, the electric polarisability (alpha) of Escherichia coli could be monitored as small quantities of antibiotics were added to the suspensions. The effect of the presence of quite small quantities of antibiotic on the electrical polarisability, which gave rise to the induced dipole, was dramatic. From the hypothesis that alpha had its origins in the bacteria-solvent interface, a theory is presented which adequately accounts for both alpha and its changes in the presence of these antibiotics. The study is taken to suggest that the antibiotic molecules were adsorbed on to the bacterial surface thereby reducing the surface charge. This in turn reduces the number of counterions and the apparent induced dipole moment. Because the electric-field scattering method is both quick and sensitive to changes in alpha, it may prove a valuable method for the study of antibiotic action on  cell and microorganism surfaces."}
{"id": "PMID:1093571", "title": "Application of latex microspheres in the isolation of plasma membranes. Affinity density perturbation of erythrocyte membranes.", "content": "Immunolatex spheres, originally developed as visual markers for scanning electron microscopy, were employed as membrane density perturbation reagents. Methacrylate spheres were bound to antibody molecules and used to label antigens on erythrocytes. Ghosts prepared from labeled cells were subjected to isopycnic centrifugation on continuous sucrose and dextran gradients. It was found that the labeled erythrocyte membranes had a substantially higher density than unlabeled membranes. The extent to which the membrane density was shifted on a given gradient depended on the number, size and density of the latex spheres and could be closely predicted by theory. These results suggest that the reagents and techniques described here have potential application for the isolation of plasma membranes from more complex cell types.", "contents": "Application of latex microspheres in the isolation of plasma membranes. Affinity density perturbation of erythrocyte membranes. Immunolatex spheres, originally developed as visual markers for scanning electron microscopy, were employed as membrane density perturbation reagents. Methacrylate spheres were bound to antibody molecules and used to label antigens on erythrocytes. Ghosts prepared from labeled cells were subjected to isopycnic centrifugation on continuous sucrose and dextran gradients. It was found that the labeled erythrocyte membranes had a substantially higher density than unlabeled membranes. The extent to which the membrane density was shifted on a given gradient depended on the number, size and density of the latex spheres and could be closely predicted by theory. These results suggest that the reagents and techniques described here have potential application for the isolation of plasma membranes from more complex cell types."}
{"id": "PMID:1093572", "title": "Energy requirements for the uptake of L-leucine by Saccharomyces cerevisiae.", "content": "(1) Substrates capable of activating mitochondrial electron transfer and oxidative phosphorylation, namely, pyruvate, acetate, propionaldehyde and butanol, stimulated the concentrative uptake (transport and accumulation) of L-[14-C]leucine by Saccharomyces cerevisiae (wild type strain 207, starved cells). Under adequate experimental conditions, the L-[14-C]leucine uptake versus the oxygen uptake ratio was almost the same with either pyruvate, acetate or D-glucose as energy sources. Substrate oxidation also increased L-[14-C]leucine incorporation into the cell protein. (2) With S. cerevisiae D261 and D247-2 and propionaldehyde as an energy source, or with strain 207 and glucose as energy source, 2,4-dinitrophenol (50 muM) inhibited L-[14-C]leucine uptake, the inhibition being accompanied by stimulation of respiration. With S. cerevisiae 207 and propionaldehyde as energy source, 2,4-dinitrophenol inhibited both respiration and L-[14-C]leucine uptake, but with respiration being less affected than uptake. Displacement of accumulated L-[14-C]leucine was also inhibited by 2,4-dinitrophenol. (3) In the presence of glucose, and for relatively brief incubation periods, anaerobically grown cells of S. cerevisiae 207 and of a p-minus \"petite\" mutant of this strain incorporated L-[14-C]leucine with less efficiency than the original wild type strain 207, grown aerobically. With D-glucose as energy source, 2,4-dinitrophenol and iodoacetate inhibited alike L-[14-C]leucine uptake by the respiration competent cells. (4) It is postulated that in respiration-competent yeasts, the mitochondrion contributes to 6-[14-C]leucine uptake by supplying high-energy compounds required for amino acid transport and accumulation. Conversely, the promitochondrion in the anaerobically grown yeast, or the modified mitochondrion in the respiratory deficient mutant, competes for high energy compounds generated by glycolysis in the cytosol.", "contents": "Energy requirements for the uptake of L-leucine by Saccharomyces cerevisiae. (1) Substrates capable of activating mitochondrial electron transfer and oxidative phosphorylation, namely, pyruvate, acetate, propionaldehyde and butanol, stimulated the concentrative uptake (transport and accumulation) of L-[14-C]leucine by Saccharomyces cerevisiae (wild type strain 207, starved cells). Under adequate experimental conditions, the L-[14-C]leucine uptake versus the oxygen uptake ratio was almost the same with either pyruvate, acetate or D-glucose as energy sources. Substrate oxidation also increased L-[14-C]leucine incorporation into the cell protein. (2) With S. cerevisiae D261 and D247-2 and propionaldehyde as an energy source, or with strain 207 and glucose as energy source, 2,4-dinitrophenol (50 muM) inhibited L-[14-C]leucine uptake, the inhibition being accompanied by stimulation of respiration. With S. cerevisiae 207 and propionaldehyde as energy source, 2,4-dinitrophenol inhibited both respiration and L-[14-C]leucine uptake, but with respiration being less affected than uptake. Displacement of accumulated L-[14-C]leucine was also inhibited by 2,4-dinitrophenol. (3) In the presence of glucose, and for relatively brief incubation periods, anaerobically grown cells of S. cerevisiae 207 and of a p-minus \"petite\" mutant of this strain incorporated L-[14-C]leucine with less efficiency than the original wild type strain 207, grown aerobically. With D-glucose as energy source, 2,4-dinitrophenol and iodoacetate inhibited alike L-[14-C]leucine uptake by the respiration competent cells. (4) It is postulated that in respiration-competent yeasts, the mitochondrion contributes to 6-[14-C]leucine uptake by supplying high-energy compounds required for amino acid transport and accumulation. Conversely, the promitochondrion in the anaerobically grown yeast, or the modified mitochondrion in the respiratory deficient mutant, competes for high energy compounds generated by glycolysis in the cytosol."}
{"id": "PMID:1093573", "title": "Demonstration of cells with igg receptor in human placenta.", "content": "The presence of two types of cells with IgG surface receptors was demonstrated in mature and immature human placentas. One type corresponded morphologically to the Hofbauer cells and the second to the blood monocytes. Transitional forms between monocytes and Hofbauer cells were also observed.", "contents": "Demonstration of cells with igg receptor in human placenta. The presence of two types of cells with IgG surface receptors was demonstrated in mature and immature human placentas. One type corresponded morphologically to the Hofbauer cells and the second to the blood monocytes. Transitional forms between monocytes and Hofbauer cells were also observed."}
{"id": "PMID:1093574", "title": "Possible mechanism of E. coli messenger RNA degration: non-enzymatic degradation.", "content": "The present paper points out lack of evidence to support the presently prevailing concept that E. coli mRNA turnover, in the gene expression process, cannot take place without mRNase(s). The present paper draws attention to possible physicochemical factors involved in the degradation, and advances a notion of non-enzymatic spontaneous degradation of E. coli mRNA in its expression process. This suggested hypothesis helps to explain hitherto reported findings on the mode of E. coli mRNA degradation.", "contents": "Possible mechanism of E. coli messenger RNA degration: non-enzymatic degradation. The present paper points out lack of evidence to support the presently prevailing concept that E. coli mRNA turnover, in the gene expression process, cannot take place without mRNase(s). The present paper draws attention to possible physicochemical factors involved in the degradation, and advances a notion of non-enzymatic spontaneous degradation of E. coli mRNA in its expression process. This suggested hypothesis helps to explain hitherto reported findings on the mode of E. coli mRNA degradation."}
{"id": "PMID:1093579", "title": "[Quantitative behavior of immunoglobulins in Hodgkin patients following splenectomy].", "content": "The values of immunoglobulins in 14 patients with malignant lymphoma were determined before and 1 to 44 weeks following splenectomy. The IgM-fraction showed a significant decrease in all cases, whereas the IgG- and IgA-fraction showed no change in its concentrations. The reason for the IgM-decrease is probably due to the absence of IgM-producing cells in the spleen.", "contents": "[Quantitative behavior of immunoglobulins in Hodgkin patients following splenectomy]. The values of immunoglobulins in 14 patients with malignant lymphoma were determined before and 1 to 44 weeks following splenectomy. The IgM-fraction showed a significant decrease in all cases, whereas the IgG- and IgA-fraction showed no change in its concentrations. The reason for the IgM-decrease is probably due to the absence of IgM-producing cells in the spleen."}
{"id": "PMID:1093600", "title": "The radiological diagnosis of adrenal tumours.", "content": "The radiological diagnosis of adrenal tumours is reviewed with special reference to the literature and the author's personal experience. The clinical presentation varies widely with the different pathology and radiological investigation is decided largely by clinical assessment. The various methods of investigation are related to the individual problems with special reference to arteriography and phlebography. Thirteen cases of Conn's tumour have been diagnosed by adrenal phlebography and 18 cases of phaeochromocytoma have been investigated by arteriography. These cases are reviewed in detail.", "contents": "The radiological diagnosis of adrenal tumours. The radiological diagnosis of adrenal tumours is reviewed with special reference to the literature and the author's personal experience. The clinical presentation varies widely with the different pathology and radiological investigation is decided largely by clinical assessment. The various methods of investigation are related to the individual problems with special reference to arteriography and phlebography. Thirteen cases of Conn's tumour have been diagnosed by adrenal phlebography and 18 cases of phaeochromocytoma have been investigated by arteriography. These cases are reviewed in detail."}
{"id": "PMID:1093601", "title": "Continuous perfusion of human cadaveric kidneys for transplantation.", "content": "63 cadaveric kidneys have been preserved for up to 36 hours by continuous perfusion on a Gambro machine 52 kidneys were transplanted: 13 (25%) functioned immediately, 23 (44%) functioned after 2 to 28 days, and 16 (31%) failed to function. 11 kidneys with poor perfusion flow characteristics were discarded. Of 30 kidneys followed for more than 18 months, graft survival was 50%. Analysis of warm and cold ischaemia data, of perfusion flow rates and biochemical studies, and comparison of function of 18 pairs of kidneys only one of which was continuously perfused, suggests that this method of preservation effectively extends the preservation time to 36 hours, but does not improve the immediate function and non-function rates, and does not provide an accurate assessment of viability.", "contents": "Continuous perfusion of human cadaveric kidneys for transplantation. 63 cadaveric kidneys have been preserved for up to 36 hours by continuous perfusion on a Gambro machine 52 kidneys were transplanted: 13 (25%) functioned immediately, 23 (44%) functioned after 2 to 28 days, and 16 (31%) failed to function. 11 kidneys with poor perfusion flow characteristics were discarded. Of 30 kidneys followed for more than 18 months, graft survival was 50%. Analysis of warm and cold ischaemia data, of perfusion flow rates and biochemical studies, and comparison of function of 18 pairs of kidneys only one of which was continuously perfused, suggests that this method of preservation effectively extends the preservation time to 36 hours, but does not improve the immediate function and non-function rates, and does not provide an accurate assessment of viability."}
{"id": "PMID:1093602", "title": "The technique and results of one-stages island patch urethroplasty.", "content": "27 one-stage island patch urethroplasties have been performed with the longest follow-up of 3 years. The method seems to have most of the advantages of standard 2-stages scrotal flap urethroplasty, and is suited to all but the most difficult and complicated strictures at any site in the urethra.", "contents": "The technique and results of one-stages island patch urethroplasty. 27 one-stage island patch urethroplasties have been performed with the longest follow-up of 3 years. The method seems to have most of the advantages of standard 2-stages scrotal flap urethroplasty, and is suited to all but the most difficult and complicated strictures at any site in the urethra."}
{"id": "PMID:1093603", "title": "A controlled clinical trial of three different methods of perineal wound management following excision of the rectum.", "content": "Three methods of perineal wound closure were studied in a randomized prospective clinical trial in 106 patients undergoing excision of the rectum for inflammatory or malignant disease. In method 2 the perineal wound was managed by open drainage. In both methods 2 and 3 the perineal wound was closed by primary suture, suprapubic suction drains being left down to the presacral space, but in method 2 the pelvic peritoneal floor was reconstituted, whilst in method 3 it was left unsutured. Topical and systemic antibiotics were administered to all patients submitted to primary closure of the perineal wound but not routinely to those whose perineal wound was left open. The overall operative mortality was 8-5 per cent but no deaths could be definitely attributed to perineal wound complications. The incidence of primary wound healing after methods 2 and 3 was 45-2 and 42-9 per cent respectively. Operative contamination of the perineal wound had an adverse effect on perineal wound healing. The incidence of persistent wound sinuses and unhealed wounds at 3 and 6 months after primary wound closure was rather less than that encountered after open drainage. It was concluded that primary closure of the perineal wound is the treatment of choice following excision of the rectum, except in cases in which contamination of the perineal wound has occurred during operation or the final state of haemostasis in the pelvis with diathermy and suture at the conclusion of the dissection was deemed to be unsatisfactory.", "contents": "A controlled clinical trial of three different methods of perineal wound management following excision of the rectum. Three methods of perineal wound closure were studied in a randomized prospective clinical trial in 106 patients undergoing excision of the rectum for inflammatory or malignant disease. In method 2 the perineal wound was managed by open drainage. In both methods 2 and 3 the perineal wound was closed by primary suture, suprapubic suction drains being left down to the presacral space, but in method 2 the pelvic peritoneal floor was reconstituted, whilst in method 3 it was left unsutured. Topical and systemic antibiotics were administered to all patients submitted to primary closure of the perineal wound but not routinely to those whose perineal wound was left open. The overall operative mortality was 8-5 per cent but no deaths could be definitely attributed to perineal wound complications. The incidence of primary wound healing after methods 2 and 3 was 45-2 and 42-9 per cent respectively. Operative contamination of the perineal wound had an adverse effect on perineal wound healing. The incidence of persistent wound sinuses and unhealed wounds at 3 and 6 months after primary wound closure was rather less than that encountered after open drainage. It was concluded that primary closure of the perineal wound is the treatment of choice following excision of the rectum, except in cases in which contamination of the perineal wound has occurred during operation or the final state of haemostasis in the pelvis with diathermy and suture at the conclusion of the dissection was deemed to be unsatisfactory."}
{"id": "PMID:1093604", "title": "Povidone-iodine for the control of surgical wound infection: a controlled clinical trial against topical cephaloridine.", "content": "One hundred and ninety-two operation wounds ina general surgical practice were randomly allocated to receive either topical cephaloridine or a providone-iodine spray, and the rate of wound sepsis was studied. In all types of operation cephaloridine proved superior to providone-iodine as a prophylactic, the difference reaching a significant level in potentially contaminated wounds.", "contents": "Povidone-iodine for the control of surgical wound infection: a controlled clinical trial against topical cephaloridine. One hundred and ninety-two operation wounds ina general surgical practice were randomly allocated to receive either topical cephaloridine or a providone-iodine spray, and the rate of wound sepsis was studied. In all types of operation cephaloridine proved superior to providone-iodine as a prophylactic, the difference reaching a significant level in potentially contaminated wounds."}
{"id": "PMID:1093605", "title": "Raised serum prolactin levels in amenorrhoea.", "content": "Serum prolactin levels measured by specific radio-immunoassay were over 30 mug/l in seven out of 25 women with amenorrhoea and in eight women with the amenorrhoe-galactorrhoea syndrome. There was no apparent relationship between these levels and levels of follicle-stimulating hormone, luteinizing hormones, and thyroid-stimulating hormone. Bromocriptine caused a transient fall in the proclatin levels in six out of seven cases, and in three menstruation and ovulation were restored. Estimation of serum prolactin may become important in assessing the degree of hypothalamic-pituitary dysfunction in amenorrhoea, and it may help in identifying a subgroup of patients at risk of developing a pituitary tumour or patients who may respond to specific treatment.", "contents": "Raised serum prolactin levels in amenorrhoea. Serum prolactin levels measured by specific radio-immunoassay were over 30 mug/l in seven out of 25 women with amenorrhoea and in eight women with the amenorrhoe-galactorrhoea syndrome. There was no apparent relationship between these levels and levels of follicle-stimulating hormone, luteinizing hormones, and thyroid-stimulating hormone. Bromocriptine caused a transient fall in the proclatin levels in six out of seven cases, and in three menstruation and ovulation were restored. Estimation of serum prolactin may become important in assessing the degree of hypothalamic-pituitary dysfunction in amenorrhoea, and it may help in identifying a subgroup of patients at risk of developing a pituitary tumour or patients who may respond to specific treatment."}
{"id": "PMID:1093609", "title": "Falciparum malaria semi-resistant to clindamycin.", "content": "Clindamycin, a semi-synthetic antibiotic of the lincomycin family, at a dose of 450 mg eight-hourly for three days in adults cured five out of 10 patients moderately ill with chloroquine-resistant falciparum malaria. Combination therapy with full-dose quinine and clindamycin for three days cured all four patients so treated who were followed up, and with half dosage three out of five patients were cured. Both combinations, however, caused upper gastrointestinal toxicity and appeared to potentiate both toxicity and possibly antimalarial efficacy. Colitis due to clindamycin was not observed. Sequential therapy was not toxic and could be useful in patients who have relapsed after more conventional treatment.", "contents": "Falciparum malaria semi-resistant to clindamycin. Clindamycin, a semi-synthetic antibiotic of the lincomycin family, at a dose of 450 mg eight-hourly for three days in adults cured five out of 10 patients moderately ill with chloroquine-resistant falciparum malaria. Combination therapy with full-dose quinine and clindamycin for three days cured all four patients so treated who were followed up, and with half dosage three out of five patients were cured. Both combinations, however, caused upper gastrointestinal toxicity and appeared to potentiate both toxicity and possibly antimalarial efficacy. Colitis due to clindamycin was not observed. Sequential therapy was not toxic and could be useful in patients who have relapsed after more conventional treatment."}
{"id": "PMID:1093610", "title": "Falciparum malaria cured by quinine followed by sulfadoxine-pyrimethamine.", "content": "Quinine (at least four doses given at intervals of eight to 12 hours) followed by a single dose of sulfadoxine-pyrimethamine (Fansidar) is the most effective treatment of chloroquine-resistant falciparum malaria. This regimen cured 96% of patients (302 out of 314) with an average initial parasite count of 90 X 10-9/1.", "contents": "Falciparum malaria cured by quinine followed by sulfadoxine-pyrimethamine. Quinine (at least four doses given at intervals of eight to 12 hours) followed by a single dose of sulfadoxine-pyrimethamine (Fansidar) is the most effective treatment of chloroquine-resistant falciparum malaria. This regimen cured 96% of patients (302 out of 314) with an average initial parasite count of 90 X 10-9/1."}
{"id": "PMID:1093613", "title": "Simplified computer-aided diagnosis of acute abdominal pain.", "content": "A simplified version of a system for computer-aided diagnosis of acute abdominal pain has been tested by \"new\" personnel unfamiliar with the previous system. After a two-month learning period the system proved more accurate in its diagnoses than the unaided clinician, and during the first five months of using the system the unaided clinicians' accuracy rose from 73% to 84%. When computer \"feedback\" was withdrawn the clinicians' diagnostic accuracy reverted towards the previous, \"unaided\" level. These findings further validate the concept of the computer as a potentially valuable diagnostic aid but indicate that a training period and computer feedback are important factors in its 4 use.", "contents": "Simplified computer-aided diagnosis of acute abdominal pain. A simplified version of a system for computer-aided diagnosis of acute abdominal pain has been tested by \"new\" personnel unfamiliar with the previous system. After a two-month learning period the system proved more accurate in its diagnoses than the unaided clinician, and during the first five months of using the system the unaided clinicians' accuracy rose from 73% to 84%. When computer \"feedback\" was withdrawn the clinicians' diagnostic accuracy reverted towards the previous, \"unaided\" level. These findings further validate the concept of the computer as a potentially valuable diagnostic aid but indicate that a training period and computer feedback are important factors in its 4 use."}
{"id": "PMID:1093632", "title": "Clinical applications of an automatic data handling system for sexually-transmitted diseases.", "content": "An automatic data handling system for the storage and evaluation of data from investigations on sexually-transmitted diseases has been developed. Among the clinical applications are epidemiological investigations, evaluation of diagnostic procedures, and evaluation of treatment regimens. One of the main features of the system is its flexibility, which allows the inclusion of an unlimited number of further evaluation programs. After a running-in period of about 3 years, during which time a number of changes were found to be necessary, the method has been found to satisfy medical and data processing requirements.", "contents": "Clinical applications of an automatic data handling system for sexually-transmitted diseases. An automatic data handling system for the storage and evaluation of data from investigations on sexually-transmitted diseases has been developed. Among the clinical applications are epidemiological investigations, evaluation of diagnostic procedures, and evaluation of treatment regimens. One of the main features of the system is its flexibility, which allows the inclusion of an unlimited number of further evaluation programs. After a running-in period of about 3 years, during which time a number of changes were found to be necessary, the method has been found to satisfy medical and data processing requirements."}
{"id": "PMID:1093633", "title": "Miconazole nitrate compared with chlordantoin in the treatment of vaginal candidiasis.", "content": "In a double-blind study of the treatment of vaginal candidiasis, miconazole nitrate gave a cure-rate of 88 per cent. (29 out of 33) compared with 58 per cent. (21 out of 36) using chlordantoin. Although approximately two-thirds of the patients who responded did so to a 10-day course of treatment, the different rates of cure with these preparations at this stage were not statistically significant. However, when a second course of treatment was given to those women who still had proven candidiasis, the overall cure-rate with miconazole was significantly greater than that with chlordantoin.", "contents": "Miconazole nitrate compared with chlordantoin in the treatment of vaginal candidiasis. In a double-blind study of the treatment of vaginal candidiasis, miconazole nitrate gave a cure-rate of 88 per cent. (29 out of 33) compared with 58 per cent. (21 out of 36) using chlordantoin. Although approximately two-thirds of the patients who responded did so to a 10-day course of treatment, the different rates of cure with these preparations at this stage were not statistically significant. However, when a second course of treatment was given to those women who still had proven candidiasis, the overall cure-rate with miconazole was significantly greater than that with chlordantoin."}
{"id": "PMID:1093634", "title": "Comparison of the treatment of herpes genitalis in men with proflavine photoinactivation, idoxuridine ointment, and normal saline.", "content": "36 male patients with genital infection by HSV confirmed by culture were each allocated to one of three treatment groups: (1) Proflavine photoinactivation, (2) 0.5 per cent. idoxuridine ointment (IDU), (3) Normal saline. They were assessed objectively at each attendance by measurement of the lesions with an operating microscope fitted with a measuring grid in one eyepiece. Material for culture for HSV was taken at each visit; the presence of symptoms (pain, discomfort, and irritation) was noted. The areas of lesions in the proflavine photoinactivation group remained larger significantly longer than in the other groups, the healing time was slower, and HSV could be isolated for longer. It is concluded that proflavine photoinactivation is of no greater value than 0.5 per cent. IDU or normal saline in the treatment of genital infection by HSV in the male.", "contents": "Comparison of the treatment of herpes genitalis in men with proflavine photoinactivation, idoxuridine ointment, and normal saline. 36 male patients with genital infection by HSV confirmed by culture were each allocated to one of three treatment groups: (1) Proflavine photoinactivation, (2) 0.5 per cent. idoxuridine ointment (IDU), (3) Normal saline. They were assessed objectively at each attendance by measurement of the lesions with an operating microscope fitted with a measuring grid in one eyepiece. Material for culture for HSV was taken at each visit; the presence of symptoms (pain, discomfort, and irritation) was noted. The areas of lesions in the proflavine photoinactivation group remained larger significantly longer than in the other groups, the healing time was slower, and HSV could be isolated for longer. It is concluded that proflavine photoinactivation is of no greater value than 0.5 per cent. IDU or normal saline in the treatment of genital infection by HSV in the male."}
{"id": "PMID:1093636", "title": "Clinical value of the Treponema pallidum haemagglutination test.", "content": "In 1,129 patients attending the Department for Sexually Transmitted Diseases, the serum was examined by three screening tests (VDRL slide, RPCF, and TPHA) and twelve cases of syphilis (1 per cent. of patients attenting the clinic) were discovered. Six of these patients were considered to have latent syphilis (5 acquired, 1 congenital) and were detected only by the TPHA; all six cases were confirmed by the FTA-ABS, The TPHA failed to detect three of the remaining six cases (2 primary and 1 very early, the latter in a contact of a patient with primary syphilis). All six cases were, however, detected by the VDRL. In seven cases, the TPHA was positive in the absence of other evidence of present or previous syphilis. In these cases the FTA-ABS was also negative. The clinical application of the TPHA test in the detection of syphilis is discussed.", "contents": "Clinical value of the Treponema pallidum haemagglutination test. In 1,129 patients attending the Department for Sexually Transmitted Diseases, the serum was examined by three screening tests (VDRL slide, RPCF, and TPHA) and twelve cases of syphilis (1 per cent. of patients attenting the clinic) were discovered. Six of these patients were considered to have latent syphilis (5 acquired, 1 congenital) and were detected only by the TPHA; all six cases were confirmed by the FTA-ABS, The TPHA failed to detect three of the remaining six cases (2 primary and 1 very early, the latter in a contact of a patient with primary syphilis). All six cases were, however, detected by the VDRL. In seven cases, the TPHA was positive in the absence of other evidence of present or previous syphilis. In these cases the FTA-ABS was also negative. The clinical application of the TPHA test in the detection of syphilis is discussed."}
{"id": "PMID:1093637", "title": "Single-dose treatment of uncomplicated gonorrhoea in males with minocycline.", "content": "Minocycline in a single 300 mg. oral dose was given to 103 men with uncomplicated gonorrhoea, of whom 85 were followed-up for at least 2 weeks. The cure rate in the 85 followed was 94.1 per cent. There were no side-effects. Thus minocycline could be considered as an effective and safe alternative to penicillin in the single-dose treatment of uncomplicated gonorrhoea in the male, but at the moment it is too expensive for routine use.", "contents": "Single-dose treatment of uncomplicated gonorrhoea in males with minocycline. Minocycline in a single 300 mg. oral dose was given to 103 men with uncomplicated gonorrhoea, of whom 85 were followed-up for at least 2 weeks. The cure rate in the 85 followed was 94.1 per cent. There were no side-effects. Thus minocycline could be considered as an effective and safe alternative to penicillin in the single-dose treatment of uncomplicated gonorrhoea in the male, but at the moment it is too expensive for routine use."}
{"id": "PMID:1093638", "title": "Comparison of oral ampicillin and doxycycline in the treatment of uncomplicated gonorrhoea.", "content": "An account is given of a computer-processed 1-year study comprising 1,124 patients (625 males and 499 females) with uncomplicated gonorrhoea. Alternate patients were treated with either two oral doses of 1 g. ampicillin 5 hours apart, or 0.3 g. doxycycline in a single oral dose. Ampicillin remained as efficacious as in 1968 to 1970, in both males and females, in whom the failure rates were 1.1 and 1 per cent. respectively. After doxycycline the failure rate was 8.1 per cent. in males, 5.7 per cent. in females, and 7.1 per cent. overall. Ampicillin was significantly better than doxycycline in the treatment of men (P less than 0.001) as well as of women (0.05 greater than P greater than 0.01). 89.7 per cent. (29/29) of the relapses in doxycycline-treated patients occurred in those harbouring strains sensitive to tetracycline. Sensitivity to doxycycline followed the pattern of tetracycline sensitivity. Thus sensitivity tests with these antibiotics provided no practical aid to therapy. The high incidence of nausea and vomiting in patients treated with doxycycline (12 per cent.) makes it inadvisable to increase the dose; instead, multiple doses are necessary to obtain satisfactory results. There were very few adverse reactions to ampicillin; a rash occurred in only three patients (0.5 per cent.). In the group treated with doxycycline, 31.5 per cent. of the patients infected by streptomycin-resistant strains relapsed compared with only 1.8 per cent. of patients infected by streptomycin-sensitive strains. This difference is highly significant (P less than 0.001). Thus the sensitivity of gonococcal strains to streptomycin in vitro may serve as a valuable guide to the likely outcome of treatment with tetracyclines.", "contents": "Comparison of oral ampicillin and doxycycline in the treatment of uncomplicated gonorrhoea. An account is given of a computer-processed 1-year study comprising 1,124 patients (625 males and 499 females) with uncomplicated gonorrhoea. Alternate patients were treated with either two oral doses of 1 g. ampicillin 5 hours apart, or 0.3 g. doxycycline in a single oral dose. Ampicillin remained as efficacious as in 1968 to 1970, in both males and females, in whom the failure rates were 1.1 and 1 per cent. respectively. After doxycycline the failure rate was 8.1 per cent. in males, 5.7 per cent. in females, and 7.1 per cent. overall. Ampicillin was significantly better than doxycycline in the treatment of men (P less than 0.001) as well as of women (0.05 greater than P greater than 0.01). 89.7 per cent. (29/29) of the relapses in doxycycline-treated patients occurred in those harbouring strains sensitive to tetracycline. Sensitivity to doxycycline followed the pattern of tetracycline sensitivity. Thus sensitivity tests with these antibiotics provided no practical aid to therapy. The high incidence of nausea and vomiting in patients treated with doxycycline (12 per cent.) makes it inadvisable to increase the dose; instead, multiple doses are necessary to obtain satisfactory results. There were very few adverse reactions to ampicillin; a rash occurred in only three patients (0.5 per cent.). In the group treated with doxycycline, 31.5 per cent. of the patients infected by streptomycin-resistant strains relapsed compared with only 1.8 per cent. of patients infected by streptomycin-sensitive strains. This difference is highly significant (P less than 0.001). Thus the sensitivity of gonococcal strains to streptomycin in vitro may serve as a valuable guide to the likely outcome of treatment with tetracyclines."}
{"id": "PMID:1093642", "title": "The sources and intracerebral distribution of auditory evoked potentials in the alert rhesus monkey.", "content": "Average auditory evoked potentials (AEP) to click stimuli were recorded from chronically implanted electrodes in alert rhesus monkeys. The cortical sources of each AEP component were identified on the basis of transcortical polarity inversions. The active loci were limited to the supratemporal plane (STP) and to a region within precentral motor cortex. Small and inconstant responses were generated in the lateral superior temporal gyrus and no locally generated activity was detected in frontal granular cortex. The responses from STP were complex, with early components generated mainly within auditory koniocortex and later components in surrounding regions. Each of these components was volume-conducted widely, both above and below the STP, and they projected onto various regions of dorsolateral surface cort(x. The volume-conducted potentials from the STP summed with the potentials generated in motor cortex to produce a complex resultant waveform. Barbiturate anesthesia abolished all but the primary AEP and reduced the volume-conducted projection of this response at the cortical surface. The configuration and intracranial distribution of the monkey AEP were compared to human intracerebral and scalp AEP recordings.", "contents": "The sources and intracerebral distribution of auditory evoked potentials in the alert rhesus monkey. Average auditory evoked potentials (AEP) to click stimuli were recorded from chronically implanted electrodes in alert rhesus monkeys. The cortical sources of each AEP component were identified on the basis of transcortical polarity inversions. The active loci were limited to the supratemporal plane (STP) and to a region within precentral motor cortex. Small and inconstant responses were generated in the lateral superior temporal gyrus and no locally generated activity was detected in frontal granular cortex. The responses from STP were complex, with early components generated mainly within auditory koniocortex and later components in surrounding regions. Each of these components was volume-conducted widely, both above and below the STP, and they projected onto various regions of dorsolateral surface cort(x. The volume-conducted potentials from the STP summed with the potentials generated in motor cortex to produce a complex resultant waveform. Barbiturate anesthesia abolished all but the primary AEP and reduced the volume-conducted projection of this response at the cortical surface. The configuration and intracranial distribution of the monkey AEP were compared to human intracerebral and scalp AEP recordings."}
{"id": "PMID:1093647", "title": "Prophylactic chemotherapy with low-dosage trimethoprim-sulfamethoxazole following acute urinary tract infections in children.", "content": "The cases of 10 children with acute and recurrent infection of the urinary tract are presented. Their ages were between 7 weeks and 9 years. Five of the children had been previously treated with a combination of sulfisoxazole, ampicillin, mandelamine and nitrofurantion and four of these children had \"breakthrough\" infections. When trimethoprim-sulfamethoxazole was administered twice weekly no further infections were noted in four of these five patients. The second group of five children were started on trimethoprim-sulfamethoxazole from the start of their infection and treatment was continued twice weekly for 5 to 7 months. No recurrence of infection of the urine has been detected up to the present time. It is concluded that treatment with trimethoprim-sulfamethoxazole initiated at the outset of urinary tract infection and twice weekly is a valuable drug in children, provided the organism responsible for the infection is sensitive to this agent.", "contents": "Prophylactic chemotherapy with low-dosage trimethoprim-sulfamethoxazole following acute urinary tract infections in children. The cases of 10 children with acute and recurrent infection of the urinary tract are presented. Their ages were between 7 weeks and 9 years. Five of the children had been previously treated with a combination of sulfisoxazole, ampicillin, mandelamine and nitrofurantion and four of these children had \"breakthrough\" infections. When trimethoprim-sulfamethoxazole was administered twice weekly no further infections were noted in four of these five patients. The second group of five children were started on trimethoprim-sulfamethoxazole from the start of their infection and treatment was continued twice weekly for 5 to 7 months. No recurrence of infection of the urine has been detected up to the present time. It is concluded that treatment with trimethoprim-sulfamethoxazole initiated at the outset of urinary tract infection and twice weekly is a valuable drug in children, provided the organism responsible for the infection is sensitive to this agent."}
{"id": "PMID:1093648", "title": "Comparison of trimethoprim-sulfamethoxazole with sulfamethoxazole in urinary tract infections of children.", "content": "The effect of trimethorpim-sulfamethoxazole was compared with that of sulfamethoxazole alone in 26 children with urinary tract infection, randomly assigned according to a double-blind procedure to two equally sized groups. TMX-SMX was found to be superior in rendering the urine culture negative for the 3 months after the start of treatment. Also, over 12-month follow-up period there were fewer recurrences in the patients who received TMP-SMX but here the difference between the two groups did not reach statistical significance.", "contents": "Comparison of trimethoprim-sulfamethoxazole with sulfamethoxazole in urinary tract infections of children. The effect of trimethorpim-sulfamethoxazole was compared with that of sulfamethoxazole alone in 26 children with urinary tract infection, randomly assigned according to a double-blind procedure to two equally sized groups. TMX-SMX was found to be superior in rendering the urine culture negative for the 3 months after the start of treatment. Also, over 12-month follow-up period there were fewer recurrences in the patients who received TMP-SMX but here the difference between the two groups did not reach statistical significance."}
{"id": "PMID:1093649", "title": "Trimethorpim-sulfamethoxazole in the treatment of chronic prostatitis.", "content": "Chronic prostatis is a common condition for which evidence of bacterial etiology is often lacking, possibly because of difficulty in obtaining secretion from the diseased portion of the gland. Aids to diagnosis apart from bacteriologic studies are of little value. It has been shown that trimethoprim reaches high levels in the prostatic fluid and when combined with sulfamethoxazole provides a highly effective antibacterial agent. In a survey of 40 patients with chronic prostatitis treated with TMP-SMX the usefulness of the combination was demonstrated.", "contents": "Trimethorpim-sulfamethoxazole in the treatment of chronic prostatitis. Chronic prostatis is a common condition for which evidence of bacterial etiology is often lacking, possibly because of difficulty in obtaining secretion from the diseased portion of the gland. Aids to diagnosis apart from bacteriologic studies are of little value. It has been shown that trimethoprim reaches high levels in the prostatic fluid and when combined with sulfamethoxazole provides a highly effective antibacterial agent. In a survey of 40 patients with chronic prostatitis treated with TMP-SMX the usefulness of the combination was demonstrated."}
{"id": "PMID:1093650", "title": "Treatment of salmonella carriers with trimethoprim-sulfamethoxazole.", "content": "One hundred and two patients with salmonellosis were observed, as either acute cases or asymptomatic carriers, over a span of 6 years. Twenty-eight patients were treated with antibiotics only or received no treatment; those in the treated group became negative for the infecting organisms after a mean time of 160 days, as compared with a mean of 52.4 days for those who were not treated. Seventy-four other patients were treated with frimthoprim-sulfamethoxazole (TMP-SMX) as the sole medication or after prior antibiotic therapy. Sixty of these patients (81.1%) were free from Salmonella in the mean time of 19.2 days, whereas 14 failed to respond and the condition cleared spontaneously in a mean time of 134.9 days. The poor response to treatment with TMP-SMX by children 2 years of age and younger is noted. The beneficial effects of treatment with TMP-SMX, as compared with antibiotics or no treatment at all, are examined in the light of the need to protect the public from this potentially dangerous infection.", "contents": "Treatment of salmonella carriers with trimethoprim-sulfamethoxazole. One hundred and two patients with salmonellosis were observed, as either acute cases or asymptomatic carriers, over a span of 6 years. Twenty-eight patients were treated with antibiotics only or received no treatment; those in the treated group became negative for the infecting organisms after a mean time of 160 days, as compared with a mean of 52.4 days for those who were not treated. Seventy-four other patients were treated with frimthoprim-sulfamethoxazole (TMP-SMX) as the sole medication or after prior antibiotic therapy. Sixty of these patients (81.1%) were free from Salmonella in the mean time of 19.2 days, whereas 14 failed to respond and the condition cleared spontaneously in a mean time of 134.9 days. The poor response to treatment with TMP-SMX by children 2 years of age and younger is noted. The beneficial effects of treatment with TMP-SMX, as compared with antibiotics or no treatment at all, are examined in the light of the need to protect the public from this potentially dangerous infection."}
{"id": "PMID:1093651", "title": "Treatment of gonorrhea in the male with trimethoprim-sulfamethoxazole using a one- or two-dose regimen.", "content": "One hundred and eighty-four male patients with uncomplicated gonorrhea were treated in a randomized double-blind trial using two drug regimens. The combinations used were co-trimoxazole (trimethoprim, 80 mg and sulfamethoxazole, 400 mg) and TMP-SDZ (sulfadiazine, 400 mg and trimethoprim, 80 mg). In 43 patients who received eight tablets of co-trimoxazole in a single dose the cure rate was 88%. In the 46 patients who received a second dose of eight tablets 24 hours later the cure rate was 100%. When TMP-SDZ was used according to the same schedule the respective cure rates were 85% (41 patients) and 86% (35 patients). It is suggested that the two-dose regimen with co-trimoxazole is very effective in the treatment of uncomplicated urethral gonorrhea in the male and that the single-dose regimen, although less effective, may well prove adequate in patients defaulting after the initial treatment. At the present time, and with our local conditions, this form of treatment should be reserved for patients sensitive to penicillin or whose infections are resistant to this agent. The attack rate for patients having an episode of gonorrhea in the 12-month period immediately preceding the trial bore a direct relation to the outcome of therapy. It was highest (26%) in the group with an unsatisfactory outcome and lowest(4.3%) in the group with the highest cure rate. No adverse toxic reactions to the drug were recorded.", "contents": "Treatment of gonorrhea in the male with trimethoprim-sulfamethoxazole using a one- or two-dose regimen. One hundred and eighty-four male patients with uncomplicated gonorrhea were treated in a randomized double-blind trial using two drug regimens. The combinations used were co-trimoxazole (trimethoprim, 80 mg and sulfamethoxazole, 400 mg) and TMP-SDZ (sulfadiazine, 400 mg and trimethoprim, 80 mg). In 43 patients who received eight tablets of co-trimoxazole in a single dose the cure rate was 88%. In the 46 patients who received a second dose of eight tablets 24 hours later the cure rate was 100%. When TMP-SDZ was used according to the same schedule the respective cure rates were 85% (41 patients) and 86% (35 patients). It is suggested that the two-dose regimen with co-trimoxazole is very effective in the treatment of uncomplicated urethral gonorrhea in the male and that the single-dose regimen, although less effective, may well prove adequate in patients defaulting after the initial treatment. At the present time, and with our local conditions, this form of treatment should be reserved for patients sensitive to penicillin or whose infections are resistant to this agent. The attack rate for patients having an episode of gonorrhea in the 12-month period immediately preceding the trial bore a direct relation to the outcome of therapy. It was highest (26%) in the group with an unsatisfactory outcome and lowest(4.3%) in the group with the highest cure rate. No adverse toxic reactions to the drug were recorded."}
{"id": "PMID:1093652", "title": "Trimethoprim-resistant Enterobacteriaceae in urinary tract infection.", "content": "The incidence of trimethoprim-resistant Enterobacteriaceae has not increased since the introduction of the combination trimethoprim-sulfamethoxazole (TMP-SMX) into the clinical use at our centre in 1973. Using the minimum inhibitory concentration (MIC) as the index of trimethoprim resistance, this ranged from 1.6 to 800 mug/ml; for the majority of isolates it lay between 1.6 and 12.5 mug/ml. About half of these trimethoprim-resistant organisms were sensitive to sulfonamide. In vitro data suggest that organisms resistant to sulfonamide as well as to trimethoprim, where the MIC for the former drug is 3.1 mug/ml or less, will be susceptible to the combination. More resistant organisms, i.e., those for which the MIC of trimethoprim is 6.2 mug/ml or more, often appear quite resistent to the combination. There is no evidence that previous therapy with TMP-SMX is a significant predisposing factor to infection with these organisms, although there is a significant correlation between previous TMP-SMX therapy and infection with organisms with a high level of trimethoprim resistance. Organisms harbouring R-factor resistance or thymine-dependent mutants were not encountered during the course of this study.", "contents": "Trimethoprim-resistant Enterobacteriaceae in urinary tract infection. The incidence of trimethoprim-resistant Enterobacteriaceae has not increased since the introduction of the combination trimethoprim-sulfamethoxazole (TMP-SMX) into the clinical use at our centre in 1973. Using the minimum inhibitory concentration (MIC) as the index of trimethoprim resistance, this ranged from 1.6 to 800 mug/ml; for the majority of isolates it lay between 1.6 and 12.5 mug/ml. About half of these trimethoprim-resistant organisms were sensitive to sulfonamide. In vitro data suggest that organisms resistant to sulfonamide as well as to trimethoprim, where the MIC for the former drug is 3.1 mug/ml or less, will be susceptible to the combination. More resistant organisms, i.e., those for which the MIC of trimethoprim is 6.2 mug/ml or more, often appear quite resistent to the combination. There is no evidence that previous therapy with TMP-SMX is a significant predisposing factor to infection with these organisms, although there is a significant correlation between previous TMP-SMX therapy and infection with organisms with a high level of trimethoprim resistance. Organisms harbouring R-factor resistance or thymine-dependent mutants were not encountered during the course of this study."}
{"id": "PMID:1093653", "title": "In vitro sensitivity of hemophilus influenzae and streptococcus pyogenes to co-trimoxazole.", "content": "The invitro testing of Hemophilus influenzae and Streptococcus pyogenes for co-trimoxazole sensitivity requires certain \"defined\" media that have to be free of inhibitory substances. The use of Columbia agar base with Fildes extract for H. influenzae or of blood agar for S. pyogenes may produce \"false-resistant\" strains. The addition of thymidine phosphorylases in the form of gentlylysed horse blood (2 to 10%) does not remove all inhibitors in those tests, especially where \"undefined\" agar bases are used, and results in scanty growth of H. influenzae; the addition of more than 2% results in dark plates, making reading of sensitivities difficult. Fildes agar for testing H. influenzae may be made with enriched sheep or horse blood if the proper \"defined\" agar base is used. The use of Wellcotest or DST (Oxoid) agar is recommended with Fildes extract for H. influenzae or with blood for S. pyogenes for in vitro testing for co-trimoxazole sensitivity. The addition of thymidine phosphorylase in the form of 2% lysed horse blood does not interfere with reading. However, it results in scanty growth of H. influenzae. Proper inoculation of plates is important. The growth on the plates should be light, dense, but not confluent. Heavy growth may render some strains \"false-resistant\" even when defined media are used. Our results indicate that many of the previously reported resistant strains of H. influenzae and S. pyogenes may have been \"false-resistant\" because of the use of \"undefined\" media. We believe that, in view of our results, respiratory infections may be treated with co-trimoxazole until bacteriologic studies prove that this treatment is contraindicated, since H. influenzae and S. pyogenes are usually found sensitive in vitro under proper conditions.", "contents": "In vitro sensitivity of hemophilus influenzae and streptococcus pyogenes to co-trimoxazole. The invitro testing of Hemophilus influenzae and Streptococcus pyogenes for co-trimoxazole sensitivity requires certain \"defined\" media that have to be free of inhibitory substances. The use of Columbia agar base with Fildes extract for H. influenzae or of blood agar for S. pyogenes may produce \"false-resistant\" strains. The addition of thymidine phosphorylases in the form of gentlylysed horse blood (2 to 10%) does not remove all inhibitors in those tests, especially where \"undefined\" agar bases are used, and results in scanty growth of H. influenzae; the addition of more than 2% results in dark plates, making reading of sensitivities difficult. Fildes agar for testing H. influenzae may be made with enriched sheep or horse blood if the proper \"defined\" agar base is used. The use of Wellcotest or DST (Oxoid) agar is recommended with Fildes extract for H. influenzae or with blood for S. pyogenes for in vitro testing for co-trimoxazole sensitivity. The addition of thymidine phosphorylase in the form of 2% lysed horse blood does not interfere with reading. However, it results in scanty growth of H. influenzae. Proper inoculation of plates is important. The growth on the plates should be light, dense, but not confluent. Heavy growth may render some strains \"false-resistant\" even when defined media are used. Our results indicate that many of the previously reported resistant strains of H. influenzae and S. pyogenes may have been \"false-resistant\" because of the use of \"undefined\" media. We believe that, in view of our results, respiratory infections may be treated with co-trimoxazole until bacteriologic studies prove that this treatment is contraindicated, since H. influenzae and S. pyogenes are usually found sensitive in vitro under proper conditions."}
{"id": "PMID:1093655", "title": "Comparison of ampicillin and trimethoprim-sulfamethoxazole in the short-term treatment of urinary tract infection.", "content": "Two groups, each of 20 patients, with urinary tract infection were randomly chosen and treated according to a double-blind procedure with either ampicillin, 500 mg, or trimethoprimsulfamethoxazole, either drug being given 4 times daily for 10 days. A number of features of the infections were studied: the occurrence of single or multiple attacks, the presence or absence of complications whether the lower or upper urinary tract was affected, favourably with ampicillin in sterilizing the urine of patients and the bacteria involved. Trimethoprim-sulfamethoxazole was found to compare with multiple and complicated urinary tract infections during a follow-up period of 3 months.", "contents": "Comparison of ampicillin and trimethoprim-sulfamethoxazole in the short-term treatment of urinary tract infection. Two groups, each of 20 patients, with urinary tract infection were randomly chosen and treated according to a double-blind procedure with either ampicillin, 500 mg, or trimethoprimsulfamethoxazole, either drug being given 4 times daily for 10 days. A number of features of the infections were studied: the occurrence of single or multiple attacks, the presence or absence of complications whether the lower or upper urinary tract was affected, favourably with ampicillin in sterilizing the urine of patients and the bacteria involved. Trimethoprim-sulfamethoxazole was found to compare with multiple and complicated urinary tract infections during a follow-up period of 3 months."}
{"id": "PMID:1093656", "title": "Pharmacokinetics of trimethoprim-sulfamethoxazole in children.", "content": "The present report extends experience with the use of trimethoprim-sulfamethoxazole (TMP-SMX) in children aged 3 months to 10 years. The regimen was TMP (200 mg)--SMX (1000 mg)/m-2d given in two equal doses. The drug was easily administered, well tolerated and efficacious in the treatment of a variety of infections in 12 children. A steady state had been achieved by the third dose of medication and accumulation of either component during days 1 through 4 did not occur. Serum concentrations of TMP were slightly lower in children aged less than 3 years compared with those aged 3 to 6 years but the differences were small and these results are preliminary. Peak mean serum TMP concentration was highest at day 3 when it reached 1.63 mug/ml. It is concluded that this regimen may be suboptimal for some major parenchymal infections even though the therapeutic result was excellent in most children.", "contents": "Pharmacokinetics of trimethoprim-sulfamethoxazole in children. The present report extends experience with the use of trimethoprim-sulfamethoxazole (TMP-SMX) in children aged 3 months to 10 years. The regimen was TMP (200 mg)--SMX (1000 mg)/m-2d given in two equal doses. The drug was easily administered, well tolerated and efficacious in the treatment of a variety of infections in 12 children. A steady state had been achieved by the third dose of medication and accumulation of either component during days 1 through 4 did not occur. Serum concentrations of TMP were slightly lower in children aged less than 3 years compared with those aged 3 to 6 years but the differences were small and these results are preliminary. Peak mean serum TMP concentration was highest at day 3 when it reached 1.63 mug/ml. It is concluded that this regimen may be suboptimal for some major parenchymal infections even though the therapeutic result was excellent in most children."}
{"id": "PMID:1093657", "title": "Comparative efficacy of ampicillin and trimethoprim-sulfamethoxazole in otitis media.", "content": "Seventy-nine children with acute otitis media were the subjects in a study designed to compare the effectiveness of ampicillin and trimethoprim-sulfamethoxazole in this infection. They received either of these two agents according to a double-blind randomized procedure that also took the child's weight into account. No significant difference was found in the clinical outcome between the two treatment regimens. Undesirable side effects from TMP-SMX were notably few.", "contents": "Comparative efficacy of ampicillin and trimethoprim-sulfamethoxazole in otitis media. Seventy-nine children with acute otitis media were the subjects in a study designed to compare the effectiveness of ampicillin and trimethoprim-sulfamethoxazole in this infection. They received either of these two agents according to a double-blind randomized procedure that also took the child's weight into account. No significant difference was found in the clinical outcome between the two treatment regimens. Undesirable side effects from TMP-SMX were notably few."}
{"id": "PMID:1093658", "title": "A comparison of trimethorprim-sulfamethoxazole with sulfamethoxazole alone in infections localized to the kidneys.", "content": "Ninety patients with urinary tract infections were treated in a randomized double-blind study with either a combination of trimethoprim and sulfamethoxazole (TMP-SMX) or sulfamethoxazole alone (SMX). Thirty of 42 patients treated with TMP-SMX were cured by the time of follow-up compared with 26 of 48 treated with SMX alone. Of the 29 patients infected with SMX-resistent organisms, the combination TMP-SMX cured 12 of 17, whereas SMX alone cured 2 of 12. Of the 61 patients infected with SMX-sensitive organisms, TMP-SMX cured 18 of 25; SMX alone cured 24 of 36. In 50 women the infection was found localized to The upper urinary tract by the use of the Fairley bladder washout technique. TMPsmx cured 16 or 24 of these patients with proved upper tract infections and SMX alone cured 11 of 26. Although none of these differences were significant, TMP-SMX appears to be an effective drug combination for the therapy of proved upper tract infection and is also effective in eradicating sulfonamide-resistant organisms.", "contents": "A comparison of trimethorprim-sulfamethoxazole with sulfamethoxazole alone in infections localized to the kidneys. Ninety patients with urinary tract infections were treated in a randomized double-blind study with either a combination of trimethoprim and sulfamethoxazole (TMP-SMX) or sulfamethoxazole alone (SMX). Thirty of 42 patients treated with TMP-SMX were cured by the time of follow-up compared with 26 of 48 treated with SMX alone. Of the 29 patients infected with SMX-resistent organisms, the combination TMP-SMX cured 12 of 17, whereas SMX alone cured 2 of 12. Of the 61 patients infected with SMX-sensitive organisms, TMP-SMX cured 18 of 25; SMX alone cured 24 of 36. In 50 women the infection was found localized to The upper urinary tract by the use of the Fairley bladder washout technique. TMPsmx cured 16 or 24 of these patients with proved upper tract infections and SMX alone cured 11 of 26. Although none of these differences were significant, TMP-SMX appears to be an effective drug combination for the therapy of proved upper tract infection and is also effective in eradicating sulfonamide-resistant organisms."}
{"id": "PMID:1093659", "title": "Hepatitis B antigen (HBAg) infection in a hemodialysis unit. I. HL-A8 and immune response to HBAg.", "content": "A total of 28 hemodialysis patients and 23 healthy individuals working in close association with the patients or their blood samples were found to possess either HBAg (22) or anti-HBAg (29). HL-A typing revealed the lack of HL-AB in the group with anti-HBAg, suggesting that the immune response to HBAg may be negatively associated with HL-AB.", "contents": "Hepatitis B antigen (HBAg) infection in a hemodialysis unit. I. HL-A8 and immune response to HBAg. A total of 28 hemodialysis patients and 23 healthy individuals working in close association with the patients or their blood samples were found to possess either HBAg (22) or anti-HBAg (29). HL-A typing revealed the lack of HL-AB in the group with anti-HBAg, suggesting that the immune response to HBAg may be negatively associated with HL-AB."}
{"id": "PMID:1093665", "title": "Immunotherapy of prostatic carcinoma with bacillus Calmette-Guerin.", "content": "Seventeen patients with histologically proven adenocarcinoma of the prostate were selected for evaluation of their immunologic competence and therapy with bacillus Calmette-Gu\u00e9rin (BCG). All patients were in stage D. The immune response was explored in two main aspects: cell-mediated and humoral immunity. Delayed skin hypersensitivity reaction with purified protein derivative (PPD), streptokinase-streptodornase (varidase), Candida, mumps antigen, and Trichophyton were tested. Lymphocyte reactivity was measured by in vitro blastogenesis. Serum immunoglobulin levels and serum protein electrophoresis were also measured. The patients were then divided in two groups according to the skin test response to PPD. Group 1 (PPD-positive) consisted of seven patients. Group 2 (PPD-negative) consisted of ten patients. In group 1, two patients were treated with intraprostatic injection of BCG every week in the following doses: 1 cc the first week, 2 cc the second week, 4 cc the third week, and 6 cc the fourth week. The five remaining patients in this group received only 1 cc every week for 4 weeks. The group 2 patients (PPD-negative) were stimulated by oral intake of BCG in an attempt to convert their skin tests to positive. All patients revealed varying degrees of immunodepression. None of the patients in group 2 (stimulated by oral intake of BCG) converted to positive skin tests. Three patients in group 1 treated with BCG showed necrosis of the tumor. The different aspects of immunodepression in this disease are analyzed and correlated to the clinical staging, histologic grading, and response to therapy. The mechanism of BCG action in advanced prostatic carcinoma is discussed.", "contents": "Immunotherapy of prostatic carcinoma with bacillus Calmette-Guerin. Seventeen patients with histologically proven adenocarcinoma of the prostate were selected for evaluation of their immunologic competence and therapy with bacillus Calmette-Gu\u00e9rin (BCG). All patients were in stage D. The immune response was explored in two main aspects: cell-mediated and humoral immunity. Delayed skin hypersensitivity reaction with purified protein derivative (PPD), streptokinase-streptodornase (varidase), Candida, mumps antigen, and Trichophyton were tested. Lymphocyte reactivity was measured by in vitro blastogenesis. Serum immunoglobulin levels and serum protein electrophoresis were also measured. The patients were then divided in two groups according to the skin test response to PPD. Group 1 (PPD-positive) consisted of seven patients. Group 2 (PPD-negative) consisted of ten patients. In group 1, two patients were treated with intraprostatic injection of BCG every week in the following doses: 1 cc the first week, 2 cc the second week, 4 cc the third week, and 6 cc the fourth week. The five remaining patients in this group received only 1 cc every week for 4 weeks. The group 2 patients (PPD-negative) were stimulated by oral intake of BCG in an attempt to convert their skin tests to positive. All patients revealed varying degrees of immunodepression. None of the patients in group 2 (stimulated by oral intake of BCG) converted to positive skin tests. Three patients in group 1 treated with BCG showed necrosis of the tumor. The different aspects of immunodepression in this disease are analyzed and correlated to the clinical staging, histologic grading, and response to therapy. The mechanism of BCG action in advanced prostatic carcinoma is discussed."}
{"id": "PMID:1093666", "title": "Comparison of adriamycin (NSC-123127) and the combination of 5-fluorouracil (NSC-19893) and cyclophosphamide (NSC-26271) in advanced prostatic cancer: a preliminary report.", "content": "A preliminary report on chemotherapy for advanced prostate malignancy is presented. Nine patients have been entered in the study, of which eight are evaluable for tumor response. Of four patients initially treated with adriamycin, only one brief (5-week) objective response was noted in a patient with soft tissue disease. No responses have been seen in four patients treated with the combination of 5-fluorouracil and cyclophosphamide. No responses have been noted in five patients who failed initial treatment and were crossed over to the other treatment. Durg toxicity has been quite acceptable.", "contents": "Comparison of adriamycin (NSC-123127) and the combination of 5-fluorouracil (NSC-19893) and cyclophosphamide (NSC-26271) in advanced prostatic cancer: a preliminary report. A preliminary report on chemotherapy for advanced prostate malignancy is presented. Nine patients have been entered in the study, of which eight are evaluable for tumor response. Of four patients initially treated with adriamycin, only one brief (5-week) objective response was noted in a patient with soft tissue disease. No responses have been seen in four patients treated with the combination of 5-fluorouracil and cyclophosphamide. No responses have been noted in five patients who failed initial treatment and were crossed over to the other treatment. Durg toxicity has been quite acceptable."}
{"id": "PMID:1093667", "title": "Clinical trial of 2-bromo-alpha-ergocryptine (NSC-169774) in human prostatic cancer.", "content": "An EORTC type I clinical trial of 2-bromo-alpha ergocryptine was performed in patients with prostatic carcinoma in clinical stages III and IV. Evaluating rules of response to the therapy were based on objective criteria. The maximum period of followup was 16 weeks. No objective remissions were observed in 24 patients, and in 13 of these patients, evidence of progression of the disease was apparent during the first 8 weeks of drug administration.", "contents": "Clinical trial of 2-bromo-alpha-ergocryptine (NSC-169774) in human prostatic cancer. An EORTC type I clinical trial of 2-bromo-alpha ergocryptine was performed in patients with prostatic carcinoma in clinical stages III and IV. Evaluating rules of response to the therapy were based on objective criteria. The maximum period of followup was 16 weeks. No objective remissions were observed in 24 patients, and in 13 of these patients, evidence of progression of the disease was apparent during the first 8 weeks of drug administration."}
{"id": "PMID:1093669", "title": "Oral estramustine phosphate (NSC-89199) in the treatment of advanced (stage D) carcinoma of the prostate.", "content": "Thirty-two patients with stage D carcinoma of the prostate were treated with oral estramustine phosphate at a dose of 15 mg/kg/day from 3 to 15 months. Objective remissions, reduction of greater than 50 percent of measurable lesions such as soft tissue masses, lymph nodes, and prostatic masses, were seen in seven of 32 patients (22 percent response rate). Subjective response, ie, relief of pain, weight gain, sense of well being, snd improved performance status, occurred in all objective responders and in seven other patients with stable disease (15 of 32 patients = 47 percent). No hematologic, hepatic, or renal toxic effects were observed. Transient nausea occurred early in one half of the patients and nausea and vomiting was dose limiting in only two patients. Oral estramustine phosphate is well tolerated and worthy of further clinical use.", "contents": "Oral estramustine phosphate (NSC-89199) in the treatment of advanced (stage D) carcinoma of the prostate. Thirty-two patients with stage D carcinoma of the prostate were treated with oral estramustine phosphate at a dose of 15 mg/kg/day from 3 to 15 months. Objective remissions, reduction of greater than 50 percent of measurable lesions such as soft tissue masses, lymph nodes, and prostatic masses, were seen in seven of 32 patients (22 percent response rate). Subjective response, ie, relief of pain, weight gain, sense of well being, snd improved performance status, occurred in all objective responders and in seven other patients with stable disease (15 of 32 patients = 47 percent). No hematologic, hepatic, or renal toxic effects were observed. Transient nausea occurred early in one half of the patients and nausea and vomiting was dose limiting in only two patients. Oral estramustine phosphate is well tolerated and worthy of further clinical use."}
{"id": "PMID:1093670", "title": "The Veterans' Administration Cooperative Urological Research Group studies of carcinoma of the prostate: a review.", "content": "The Veterans' Administration Cooperative Urological Research Group has conducted three consecutive randomized clinical trials of various treatments for all stages of prostatic carcinoma. Thus far, a total of 3774 patients have been entered into these three main studies. The first study showed that early endocrine treatment of patients with advanced prostatic cancer did not increase overall survival when compared to initial treatment with placebo alone. Diethylstilbestrol, when given in a dose of 5.0 mg/day, was associated with an increased incidence of cardiovascular deaths. In the second study, diethylstilbestrol given in a dose of 1.0 mg/day has been as effective as the 5.0-mg dose in controlling the prostatic carcinoma but has been associated with a lower incidence of cardiovascular deaths. Patients are still being entered into the third study and it is too early to report the findings in detail.", "contents": "The Veterans' Administration Cooperative Urological Research Group studies of carcinoma of the prostate: a review. The Veterans' Administration Cooperative Urological Research Group has conducted three consecutive randomized clinical trials of various treatments for all stages of prostatic carcinoma. Thus far, a total of 3774 patients have been entered into these three main studies. The first study showed that early endocrine treatment of patients with advanced prostatic cancer did not increase overall survival when compared to initial treatment with placebo alone. Diethylstilbestrol, when given in a dose of 5.0 mg/day, was associated with an increased incidence of cardiovascular deaths. In the second study, diethylstilbestrol given in a dose of 1.0 mg/day has been as effective as the 5.0-mg dose in controlling the prostatic carcinoma but has been associated with a lower incidence of cardiovascular deaths. Patients are still being entered into the third study and it is too early to report the findings in detail."}
{"id": "PMID:1093671", "title": "Clinical results with estramustine phosphate (NSC-89199): a comparison of the intravenous and oral preparations.", "content": "The clinical results with estramustine phosphate in far-advanced carcinoma of the prostate refractory to other treatments are presented. Good clinical results have been achieved in about 40 percent of the 90 patients treated and regression of metastases has occurred in about 20 percent. Estramustine phosphate in the primary treatment of far-advanced carcinoma of the prostate shows very good and promising clinical results. A total of 132 patients were treated.", "contents": "Clinical results with estramustine phosphate (NSC-89199): a comparison of the intravenous and oral preparations. The clinical results with estramustine phosphate in far-advanced carcinoma of the prostate refractory to other treatments are presented. Good clinical results have been achieved in about 40 percent of the 90 patients treated and regression of metastases has occurred in about 20 percent. Estramustine phosphate in the primary treatment of far-advanced carcinoma of the prostate shows very good and promising clinical results. A total of 132 patients were treated."}
{"id": "PMID:1093672", "title": "Serial labeling index determination as a predictor of response in human solid tumors.", "content": "A rapid method for determining labeling indices in solid tumor specimens, tumor-induced effusions, and tumor-bearing bone marrows was utilized in 116 patients. Of these, 48 patients were studied pre- and postchemotherapy. The magnitude of a significant change in labeling index (LI percent) was determined statistically. Of the 48 patients studied serially, 42 were studied 17 days or less following completion of their chemotherapy. In 26 patients without a significant change in tumor LI percent, there was no subsequent clinical response to chemotherapy. Three additional patients in this group are inevaluable at present. In 11 patients, there was a significant fall in tumor LI percent following chemotherapy. Seven of these had a 50 percent or greater regression of demonstrable disease, one patient had definite tumor effect but the effect was not a partial response and three patients were not evaluable for clinical response. In two patients there was a significant increase in tumor LI percent and the patients had rapid tumor progression and death. Predictions derived from serial study of the LI percent by this method correlate significantly with subsequent behavior of the tumors tested following chemotherapy and may prove clinically useful in making decisions about when or whether to change therapy.", "contents": "Serial labeling index determination as a predictor of response in human solid tumors. A rapid method for determining labeling indices in solid tumor specimens, tumor-induced effusions, and tumor-bearing bone marrows was utilized in 116 patients. Of these, 48 patients were studied pre- and postchemotherapy. The magnitude of a significant change in labeling index (LI percent) was determined statistically. Of the 48 patients studied serially, 42 were studied 17 days or less following completion of their chemotherapy. In 26 patients without a significant change in tumor LI percent, there was no subsequent clinical response to chemotherapy. Three additional patients in this group are inevaluable at present. In 11 patients, there was a significant fall in tumor LI percent following chemotherapy. Seven of these had a 50 percent or greater regression of demonstrable disease, one patient had definite tumor effect but the effect was not a partial response and three patients were not evaluable for clinical response. In two patients there was a significant increase in tumor LI percent and the patients had rapid tumor progression and death. Predictions derived from serial study of the LI percent by this method correlate significantly with subsequent behavior of the tumors tested following chemotherapy and may prove clinically useful in making decisions about when or whether to change therapy."}
{"id": "PMID:1093673", "title": "Effect of iodoacetate on the bone marrow immunocompetence of AKR mice.", "content": "Studies were conducted to determine whether the sulfhydryl inhibitor, sodium iodoacetate, administered to preleukemic AKR mice and to mature C3H mice altered the immunocompetence of their bone marrow. Parameters investigated included the splenic plaque-forming capacity directed to sheep erythrocytes of bone marrow transferred from iodoacetate-treated aanimals to irradiated syngeneic recipients and the mitogenic responsiveness of bone marrow cells from untreated and iodoacetate-treated preleukemic AKR mice to phytohemagglutinin and concanavalin A. The administration of two 0.5-ml doses of 10 mM iodoacetate to preleukemic AKR mice and to C3H mice resulted in a significant increase in bone marrow immunocompetence. Irradiated mice given marrow transplants from iodoacetate-treated syngeneic donors exhibited greater numbers of plaque-forming cells directed against sheep erythrocytes than did recipients of marrow from control animals. This effect was abrogated when the donor marrow was previously treated in vitro with rabbit antimouse brain serum and the complement to remove thymus-derived lymphocytes. The mitogenic responsiveness of marrow cultures from iodoacetate-treated AKR mice to phytohemagglutinin was similar to that observed for control mice, while the response to concanavalin A was decreased. These findings suggest that the administration of iodoacetate potentiated the immunocompetence of bone marrow by affecting thymus-derived cells.", "contents": "Effect of iodoacetate on the bone marrow immunocompetence of AKR mice. Studies were conducted to determine whether the sulfhydryl inhibitor, sodium iodoacetate, administered to preleukemic AKR mice and to mature C3H mice altered the immunocompetence of their bone marrow. Parameters investigated included the splenic plaque-forming capacity directed to sheep erythrocytes of bone marrow transferred from iodoacetate-treated aanimals to irradiated syngeneic recipients and the mitogenic responsiveness of bone marrow cells from untreated and iodoacetate-treated preleukemic AKR mice to phytohemagglutinin and concanavalin A. The administration of two 0.5-ml doses of 10 mM iodoacetate to preleukemic AKR mice and to C3H mice resulted in a significant increase in bone marrow immunocompetence. Irradiated mice given marrow transplants from iodoacetate-treated syngeneic donors exhibited greater numbers of plaque-forming cells directed against sheep erythrocytes than did recipients of marrow from control animals. This effect was abrogated when the donor marrow was previously treated in vitro with rabbit antimouse brain serum and the complement to remove thymus-derived lymphocytes. The mitogenic responsiveness of marrow cultures from iodoacetate-treated AKR mice to phytohemagglutinin was similar to that observed for control mice, while the response to concanavalin A was decreased. These findings suggest that the administration of iodoacetate potentiated the immunocompetence of bone marrow by affecting thymus-derived cells."}
{"id": "PMID:1093675", "title": "Localization of neuroblastoma in vivo with tumor-specific antibodies.", "content": "Studies of the mouse C-1300 neuroblastoma were undertaken in order to isolate tumor-specific antibodies and harness them for detection of tumors in vivo. Preliminary investigations demonstrated the strain-growth specificity of the neuroblastoma in A/Jax male mice and established the requirement for tumor viability for successful adoptive passage. Intradermally passaged tumor permitted extended survival of mice so that serum could be sampled at intervals for the presence of tumor-specific antibodies. By means of an indirect radioimmunoassay with glutaraldehyde-fixed identified in the serum of tumor-bearing hosts 6 days after inoculation, with a steady increase in antibody levels observed through Day 22. An eluate in which immunoglobulin G antibodies were identified by immunoelectrophoresis was obtained from purified tumor cells by acid buffer incubation. These antibodies were labeled with 125-I, absorbed with normal tissues, and injected into tumor-bearing mice. A selectively collimated single-probe isotope localization was positioned over the intradermal tumor, while the rest of the animal was shielded with lead. With this device, 125-neuroblastoma eluate was significantly taken up in the neuroblastoma but not in the mouse head or in a reticulum cell sarcoma control. Increasing uptake of MOPC 141 125-I-immunoglobulin G was not observed in either tumor. These studies suggest that the mouse neuroblastoma may provide a source of tumor-specific, antibodies and that, with sensitive monitoring devices, these antibodies may be utilized to localize occult neoplastic tissue in vivo.", "contents": "Localization of neuroblastoma in vivo with tumor-specific antibodies. Studies of the mouse C-1300 neuroblastoma were undertaken in order to isolate tumor-specific antibodies and harness them for detection of tumors in vivo. Preliminary investigations demonstrated the strain-growth specificity of the neuroblastoma in A/Jax male mice and established the requirement for tumor viability for successful adoptive passage. Intradermally passaged tumor permitted extended survival of mice so that serum could be sampled at intervals for the presence of tumor-specific antibodies. By means of an indirect radioimmunoassay with glutaraldehyde-fixed identified in the serum of tumor-bearing hosts 6 days after inoculation, with a steady increase in antibody levels observed through Day 22. An eluate in which immunoglobulin G antibodies were identified by immunoelectrophoresis was obtained from purified tumor cells by acid buffer incubation. These antibodies were labeled with 125-I, absorbed with normal tissues, and injected into tumor-bearing mice. A selectively collimated single-probe isotope localization was positioned over the intradermal tumor, while the rest of the animal was shielded with lead. With this device, 125-neuroblastoma eluate was significantly taken up in the neuroblastoma but not in the mouse head or in a reticulum cell sarcoma control. Increasing uptake of MOPC 141 125-I-immunoglobulin G was not observed in either tumor. These studies suggest that the mouse neuroblastoma may provide a source of tumor-specific, antibodies and that, with sensitive monitoring devices, these antibodies may be utilized to localize occult neoplastic tissue in vivo."}
{"id": "PMID:1093676", "title": "Effects of methanol extraction residue and therapeutic irradiation against established isografts and simulated local recurrence of mammary carcinomas.", "content": "Female BALB/c mice carrying established isografts or simulated local recurrence implants of 2 rapidly growing mammary adenocarcinomas were treated either by injection of the methanol extraction residue (MER) fraction of killed Bacillus Calmette-Gu\u00e9rin organisms (given s.c. or into the tumor) or by focal X-irradiation or by both. None of the modalities of therapy effected cures, but in many instances there was a significant retardation of tumor cevelopment and prolongation of the lives of the mice. Administration of MER alone offered protection in a number of cases but less often than the other forms of treatment. Combined therapy with MER and irradiation was, on the whole, the most successful therapeutic intervention. MER or irradiation administered alone enhanced the neoplastic process only on rare occasions; this appeared to be the case even more infrequently with combined treatment. MER was most likely to be effective alone or in combination when small quantities were used and when only 1 treatment or 1 cycle of combined therapy was given. The therapeutic action of MER was not dependent on direct introduction of the agent into a neoplastic focus; s.c. administration distal to the tumor site was almost always at least as satisfactory as injection directly into the tumor mass and indeed was often more efficacious.", "contents": "Effects of methanol extraction residue and therapeutic irradiation against established isografts and simulated local recurrence of mammary carcinomas. Female BALB/c mice carrying established isografts or simulated local recurrence implants of 2 rapidly growing mammary adenocarcinomas were treated either by injection of the methanol extraction residue (MER) fraction of killed Bacillus Calmette-Gu\u00e9rin organisms (given s.c. or into the tumor) or by focal X-irradiation or by both. None of the modalities of therapy effected cures, but in many instances there was a significant retardation of tumor cevelopment and prolongation of the lives of the mice. Administration of MER alone offered protection in a number of cases but less often than the other forms of treatment. Combined therapy with MER and irradiation was, on the whole, the most successful therapeutic intervention. MER or irradiation administered alone enhanced the neoplastic process only on rare occasions; this appeared to be the case even more infrequently with combined treatment. MER was most likely to be effective alone or in combination when small quantities were used and when only 1 treatment or 1 cycle of combined therapy was given. The therapeutic action of MER was not dependent on direct introduction of the agent into a neoplastic focus; s.c. administration distal to the tumor site was almost always at least as satisfactory as injection directly into the tumor mass and indeed was often more efficacious."}
{"id": "PMID:1093677", "title": "Dietary modifications affecting the mutagenicity of N-nitroso compounds in the host-mediated assay.", "content": "The effects of various dietary modifications on the mutagenicity of dimethylnitrosamine (DMNA), N-nitrosomorpholine, and N-methyl-N-nitrosourea for Salmonella typhimurium his G-46 in the host-mediated assay were studied. The diets used were:chow, complete semisynthetic, protein-free, and all-casein, in addition to a 24-hr starvation regimen. The mutagenicity of DMNA and N-nitrosomorpholine, which require metabolic activation for their biological activity, was depressed by the complete semisynthetic diet, as compared to the mutagenicity in mice fed the chow diet. DMNA mutagenicity was depressed by the protein-free diet and enhanced by pure casein as compared with the complete semisynthetic diet. N-Nitrosomorpholine mutagenicity was enhanced by starvation, but results with mice fed the protein-free and all-casein diets were ambiguous. N-Methyl-N-nitrosourea, which does not require metabolic activation for its biological activities, responded in an opposite manner to that of DMNA; its mutagenicity was enhanced by the complete semisynthetic and protein-free diets, but was depressed by the all-casein diet.", "contents": "Dietary modifications affecting the mutagenicity of N-nitroso compounds in the host-mediated assay. The effects of various dietary modifications on the mutagenicity of dimethylnitrosamine (DMNA), N-nitrosomorpholine, and N-methyl-N-nitrosourea for Salmonella typhimurium his G-46 in the host-mediated assay were studied. The diets used were:chow, complete semisynthetic, protein-free, and all-casein, in addition to a 24-hr starvation regimen. The mutagenicity of DMNA and N-nitrosomorpholine, which require metabolic activation for their biological activity, was depressed by the complete semisynthetic diet, as compared to the mutagenicity in mice fed the chow diet. DMNA mutagenicity was depressed by the protein-free diet and enhanced by pure casein as compared with the complete semisynthetic diet. N-Nitrosomorpholine mutagenicity was enhanced by starvation, but results with mice fed the protein-free and all-casein diets were ambiguous. N-Methyl-N-nitrosourea, which does not require metabolic activation for its biological activities, responded in an opposite manner to that of DMNA; its mutagenicity was enhanced by the complete semisynthetic and protein-free diets, but was depressed by the all-casein diet."}
{"id": "PMID:1093678", "title": "Secretory component in human mammary carcinoma.", "content": "Since secretory component is thought to be a normal glandular epithelial cell product, surgical specimens from patients with mammary carcinoma, an epithelial cancer, were studied with antisera to human free secretory component by indirect immunofluorescence microscopy. Normal breast tissue (10 cases) showed fluorescent epithelial cells confined to normal ducts. This was in marked contrast to ubvasive mammary carcinoma (20 cases), which showed intense staining of tumor cells and stromal cells in addition to the normal ductular  epithelium. Metastases in axillary lymph nodes (2 cases) showed intense fluorescence for secretory component, whereas axillary nodes without metastases from 2 patients with breast cancer showed no fluorescence. In both normal and tumor tissue, antiimmunoglobulin A stained only ducts and subepithelial plasma cells, thus establishing that the secretory component in tumor cells was not part of an intact secretory immunoglobulin A molecule. This finding was not restricted to mammary carcinoma, since preliminary studies of colon, lung, and bladder carcinoma also demonstrated tumor cells with cytoplasmic fluorescence for secretory component. In contrast, the tumor cells in 2 cases of sarcoma, a nonepithelial cancer, did not exhibit fluorescence for secretory component.", "contents": "Secretory component in human mammary carcinoma. Since secretory component is thought to be a normal glandular epithelial cell product, surgical specimens from patients with mammary carcinoma, an epithelial cancer, were studied with antisera to human free secretory component by indirect immunofluorescence microscopy. Normal breast tissue (10 cases) showed fluorescent epithelial cells confined to normal ducts. This was in marked contrast to ubvasive mammary carcinoma (20 cases), which showed intense staining of tumor cells and stromal cells in addition to the normal ductular  epithelium. Metastases in axillary lymph nodes (2 cases) showed intense fluorescence for secretory component, whereas axillary nodes without metastases from 2 patients with breast cancer showed no fluorescence. In both normal and tumor tissue, antiimmunoglobulin A stained only ducts and subepithelial plasma cells, thus establishing that the secretory component in tumor cells was not part of an intact secretory immunoglobulin A molecule. This finding was not restricted to mammary carcinoma, since preliminary studies of colon, lung, and bladder carcinoma also demonstrated tumor cells with cytoplasmic fluorescence for secretory component. In contrast, the tumor cells in 2 cases of sarcoma, a nonepithelial cancer, did not exhibit fluorescence for secretory component."}
{"id": "PMID:1093679", "title": "Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture.", "content": "The Nara Bladder Tumor No. 2 cell line, established from a urinary bladder carcinoma in the Wistar rat, formed keratinizing cells and multicellular pearls in meniscus gradient culture when fed with a medium of 15 or 30% fetal calf serum in Eagle's minimal essential medium. Confluent monolayer cultures were first prepared with the tubes in conventional horizontal position. When cultures were then changed to a vertical position, stratification, piling up, and aggregation of cells were observed in a few days at the aerobic end of the gradient. Keratinization appeared 1 week after the tubes were placed in vertical position. Squamous differentiation proceeded to the formation of keratin pearls, a phenomenon never observed so distinctly and in such abundance in horizontal culture. Supplements of vitamin A, as low as 1 IU/ml, added to the medium did not inhibit piling up or aggregation but did prevent keratinization. Inhibition of keratinization by vitamin A was reversible. After vitamin A was removed from the medium, the cells in the aggregates progressed to keratinization.", "contents": "Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. The Nara Bladder Tumor No. 2 cell line, established from a urinary bladder carcinoma in the Wistar rat, formed keratinizing cells and multicellular pearls in meniscus gradient culture when fed with a medium of 15 or 30% fetal calf serum in Eagle's minimal essential medium. Confluent monolayer cultures were first prepared with the tubes in conventional horizontal position. When cultures were then changed to a vertical position, stratification, piling up, and aggregation of cells were observed in a few days at the aerobic end of the gradient. Keratinization appeared 1 week after the tubes were placed in vertical position. Squamous differentiation proceeded to the formation of keratin pearls, a phenomenon never observed so distinctly and in such abundance in horizontal culture. Supplements of vitamin A, as low as 1 IU/ml, added to the medium did not inhibit piling up or aggregation but did prevent keratinization. Inhibition of keratinization by vitamin A was reversible. After vitamin A was removed from the medium, the cells in the aggregates progressed to keratinization."}
{"id": "PMID:1093694", "title": "Immuno-electron microscopic localization of luteinizing hormone-releasing hormone in the arcuate nuclei and median eminence of the rat.", "content": "Luteinizing hormone-releasing hormone (LH-RH) was localized in neurons of the arcuate nucleus and median eminence, and in many of the nerve fibres of the median eminence of the rat, by using antisera to synthetic LH-RH with the immunoglobulin-peroxidase technique or by using a soluble peroxidase-antiperoxidase complex at the electron microsc opic level. Many of the arcuate neurons and some of the median eminence neurons contained numerous densely stained granules elsewhere in the cytoplasm. Similar granules, positive for LH-RH, were observed in many nerve profiles around the capillaries of the primary portal plexus of zona palisadica and zona granulosa of the medain eminence, and such profiles may represent the release sites of LH-RH  into the portal vessels. About 20% of the nerve profiles around the primary portal plexus were LH-RH positive and the rest of the nerve profiles were negative. The negative profiles may belong to other hypophysiotropic hormones and/or to catecholaminergic fibers. Some nerve endings at the ventricular surface of the ependymal cells, in contact with the ventricular fluid, were also LH-RH positive. These nerve endings probably release their secretion into the cerebrospinal fluid. There was no staining of the mitochondria, the nuclei or the axonal membranes. Stain of varying intensity was seen in the secretory granules, and within and around the cisternae of the Golgi complex. The stained granules in the cisternae wer pleomorphic. From these observations it was concluded that the LH-RH  is packaged or condensed into granules in the Golgi cisternae.", "contents": "Immuno-electron microscopic localization of luteinizing hormone-releasing hormone in the arcuate nuclei and median eminence of the rat. Luteinizing hormone-releasing hormone (LH-RH) was localized in neurons of the arcuate nucleus and median eminence, and in many of the nerve fibres of the median eminence of the rat, by using antisera to synthetic LH-RH with the immunoglobulin-peroxidase technique or by using a soluble peroxidase-antiperoxidase complex at the electron microsc opic level. Many of the arcuate neurons and some of the median eminence neurons contained numerous densely stained granules elsewhere in the cytoplasm. Similar granules, positive for LH-RH, were observed in many nerve profiles around the capillaries of the primary portal plexus of zona palisadica and zona granulosa of the medain eminence, and such profiles may represent the release sites of LH-RH  into the portal vessels. About 20% of the nerve profiles around the primary portal plexus were LH-RH positive and the rest of the nerve profiles were negative. The negative profiles may belong to other hypophysiotropic hormones and/or to catecholaminergic fibers. Some nerve endings at the ventricular surface of the ependymal cells, in contact with the ventricular fluid, were also LH-RH positive. These nerve endings probably release their secretion into the cerebrospinal fluid. There was no staining of the mitochondria, the nuclei or the axonal membranes. Stain of varying intensity was seen in the secretory granules, and within and around the cisternae of the Golgi complex. The stained granules in the cisternae wer pleomorphic. From these observations it was concluded that the LH-RH  is packaged or condensed into granules in the Golgi cisternae."}
{"id": "PMID:1093696", "title": "Regulation of stable RNA synthesis and ppGpp levels in growing cells of Escherichia coli.", "content": "Under the balanced condition of growth of E. coli cells, no distinct difference is observed in stable RNA and protein synthesis between CP78 (rel+) and CP79 (rel minus), whereas a considerable difference is present in RNA accumulation between NF161 (rel+) and NF162 (rel minus), where NF161 smaller than NF162. The RNA content of NF161 is lower than that of NF162 in four different cultures with different growth rates. These two sets of isogenic pairs of rel+ and rel minus strains are commonly used in the study of rel gene function; however, NF161 is a mutant in the spoT gene whose product may be responsible for the degradation of ppGpp. The basal levels of ppGpp in these four strains growing with three different growth rates were examined: NF161 (rel+ spoT minus) has a much higher content of ppGpp than do other strains. Furthermore, the contents of ppGpp tend to be lower when the above four strains are growing at a faster rate. Thus a close correlation seems to exist between the content of RNA and the basal level of ppGpp under the condition of balanced growth.", "contents": "Regulation of stable RNA synthesis and ppGpp levels in growing cells of Escherichia coli. Under the balanced condition of growth of E. coli cells, no distinct difference is observed in stable RNA and protein synthesis between CP78 (rel+) and CP79 (rel minus), whereas a considerable difference is present in RNA accumulation between NF161 (rel+) and NF162 (rel minus), where NF161 smaller than NF162. The RNA content of NF161 is lower than that of NF162 in four different cultures with different growth rates. These two sets of isogenic pairs of rel+ and rel minus strains are commonly used in the study of rel gene function; however, NF161 is a mutant in the spoT gene whose product may be responsible for the degradation of ppGpp. The basal levels of ppGpp in these four strains growing with three different growth rates were examined: NF161 (rel+ spoT minus) has a much higher content of ppGpp than do other strains. Furthermore, the contents of ppGpp tend to be lower when the above four strains are growing at a faster rate. Thus a close correlation seems to exist between the content of RNA and the basal level of ppGpp under the condition of balanced growth."}
{"id": "PMID:1093748", "title": "Primary role of renal homografts in setting chronic blood pressure levels in rats.", "content": "The genotype of homograft kidneys plays the primary role in determining chronic blood pressure levels in two strains of rats with opposite genetically controlled propensities for hyptertension. In hypertensive rats from the hypertension-prone (S) strain, a renal homograft from the same strain resulted in a slight rise in blood pressure to a level that was equivalent to that in appropriate uninephrectomized S controls. In contrast, a renal homograft from the hypertension-resistant (R) strain led to a sharp fall in blood pressure in hypertensive S recipients. Opposite results were found when the host came from the R strain: R homografts maintained the same low pressure as that seen in controls, whereas S homografts resulted in hypertension. We concluded that genetically controlled factors operating through the kidney can chronically modify the blood pressure up or down. The central role of the kidney in hypertension is thus further documented.", "contents": "Primary role of renal homografts in setting chronic blood pressure levels in rats. The genotype of homograft kidneys plays the primary role in determining chronic blood pressure levels in two strains of rats with opposite genetically controlled propensities for hyptertension. In hypertensive rats from the hypertension-prone (S) strain, a renal homograft from the same strain resulted in a slight rise in blood pressure to a level that was equivalent to that in appropriate uninephrectomized S controls. In contrast, a renal homograft from the hypertension-resistant (R) strain led to a sharp fall in blood pressure in hypertensive S recipients. Opposite results were found when the host came from the R strain: R homografts maintained the same low pressure as that seen in controls, whereas S homografts resulted in hypertension. We concluded that genetically controlled factors operating through the kidney can chronically modify the blood pressure up or down. The central role of the kidney in hypertension is thus further documented."}
{"id": "PMID:1093749", "title": "Changes in myocardial blood flow and S-T segment elevation following coronary artery occlusion in dogs.", "content": "The relationship between regional blood flow and epicardial S-T segment elevation was studied in 26 open-chest anesthetized dogs with left anterior coronary artery ligations. Changes in myocardial blood flow, measured with 15 plus or minus 5mu (diameter) microspheres labeled with 141-Ce, 85-Sr, and 169-Yb, were correlated with summated S-T segment elevations 15 minutes, 1 hour, and 2 hours after coronary artery occlusion. In normal areas, myocardial blood flow was 113 plus or minus 5 ml/min 100 g- minus 1 and summated S-T segment elevation was 0.3 plus or minus 0.2 mv. Fifteen minutes after coronary artery occlusion in 26 dogs, S-T segment elevation was 5.7 plus or minus 0.7 mv over the center of the infarct and myocardial blood flow was 10 plus or minus 1 ml/min 100 g- minus 1; over the border zone, myocardial blood flow was 63 plus or minus 4 ml/min 100 g- minus 1 and S-T segment elevation was 3.1 plus or minus 0.1 mv. One third of the areas with a myocardial blood flow of 10 ml/min 100 g- minus 1 or less had no S-T segment elevation. In the center and border zones of the infarct in 9 dogs, myocardial blood flow increased from 11 plus or minus 2 and 67 plus or minus 8 ml/min 100 g- minus 1 15 minutes after occlusion to 20 plus or minus 4 and 84 plus or minus 12 ml/min 100 g- minus 1, respectively, 2 hours after coronary artery occlusion. These increases were not associated with a significant reduction in summated S-T segment elevation. The results do not suggest a simple quantitative relationship between epicardial S-T segment elevation and myocardial blood flow following acute coronary artery occlusion.", "contents": "Changes in myocardial blood flow and S-T segment elevation following coronary artery occlusion in dogs. The relationship between regional blood flow and epicardial S-T segment elevation was studied in 26 open-chest anesthetized dogs with left anterior coronary artery ligations. Changes in myocardial blood flow, measured with 15 plus or minus 5mu (diameter) microspheres labeled with 141-Ce, 85-Sr, and 169-Yb, were correlated with summated S-T segment elevations 15 minutes, 1 hour, and 2 hours after coronary artery occlusion. In normal areas, myocardial blood flow was 113 plus or minus 5 ml/min 100 g- minus 1 and summated S-T segment elevation was 0.3 plus or minus 0.2 mv. Fifteen minutes after coronary artery occlusion in 26 dogs, S-T segment elevation was 5.7 plus or minus 0.7 mv over the center of the infarct and myocardial blood flow was 10 plus or minus 1 ml/min 100 g- minus 1; over the border zone, myocardial blood flow was 63 plus or minus 4 ml/min 100 g- minus 1 and S-T segment elevation was 3.1 plus or minus 0.1 mv. One third of the areas with a myocardial blood flow of 10 ml/min 100 g- minus 1 or less had no S-T segment elevation. In the center and border zones of the infarct in 9 dogs, myocardial blood flow increased from 11 plus or minus 2 and 67 plus or minus 8 ml/min 100 g- minus 1 15 minutes after occlusion to 20 plus or minus 4 and 84 plus or minus 12 ml/min 100 g- minus 1, respectively, 2 hours after coronary artery occlusion. These increases were not associated with a significant reduction in summated S-T segment elevation. The results do not suggest a simple quantitative relationship between epicardial S-T segment elevation and myocardial blood flow following acute coronary artery occlusion."}
{"id": "PMID:1093750", "title": "Mechanism of the serotonin effect on lung transvascular fluid and protein movement in awake sheep.", "content": "To see how serotonin affects filtration from lung vessels, we measured vascular pressures, lung lymph flow, lung lymph and blood plasma protein concentrations, arterial blood gases, cardiac output, and lung water content in unanesthetized sheep before and during intravenous serotonin infusions and compared serotonin effects with the effects of inflating a balloon in the left atrium in the same sheep. Serotonin caused a dose-related increase in lung lymph flow and a dose-related decrease in lymph-plasma protein concentration ratios. Steady-state 4-hour serotonin infusions at 4 mu-g/kg min-1 caused lymph flow to increase from 5.4 plus or minus 0.7 (SE) ml/hour to 8.3 plus or minus 1.3 ml/hour, lymph-plasma albumin ratios to fall from 0.78 plus or minus 0.05 to 0.72 plus or minus 0.04, lymph-plasma globulin ratios to fall from 0.64 plus or minus to 0.56 plus or minus 0.02, and pulmonary arterial and left atrial pressures to increase by 3 cm H-20 each. Lymph clearance and permeability-surface area products for eight protein fractions ranging from 36 A to 96 A in molecular radius during steady-state serotonin infusion studies were not significantly different from those during steady-state increased pressure studies. Four-hour infusions of serotonin at 4 mu-g/kg/kg min-1 caused a moderate fall in arterial Po-2 and a slight increase in arterial pH but did not affect cardiac output or cause pulmonary edema. We conclude that serotonin increases lung transvascular filtration primarily by increasing the transmural pressure gradient in exchanging vessels rather than by increasing vascular permeability.", "contents": "Mechanism of the serotonin effect on lung transvascular fluid and protein movement in awake sheep. To see how serotonin affects filtration from lung vessels, we measured vascular pressures, lung lymph flow, lung lymph and blood plasma protein concentrations, arterial blood gases, cardiac output, and lung water content in unanesthetized sheep before and during intravenous serotonin infusions and compared serotonin effects with the effects of inflating a balloon in the left atrium in the same sheep. Serotonin caused a dose-related increase in lung lymph flow and a dose-related decrease in lymph-plasma protein concentration ratios. Steady-state 4-hour serotonin infusions at 4 mu-g/kg min-1 caused lymph flow to increase from 5.4 plus or minus 0.7 (SE) ml/hour to 8.3 plus or minus 1.3 ml/hour, lymph-plasma albumin ratios to fall from 0.78 plus or minus 0.05 to 0.72 plus or minus 0.04, lymph-plasma globulin ratios to fall from 0.64 plus or minus to 0.56 plus or minus 0.02, and pulmonary arterial and left atrial pressures to increase by 3 cm H-20 each. Lymph clearance and permeability-surface area products for eight protein fractions ranging from 36 A to 96 A in molecular radius during steady-state serotonin infusion studies were not significantly different from those during steady-state increased pressure studies. Four-hour infusions of serotonin at 4 mu-g/kg/kg min-1 caused a moderate fall in arterial Po-2 and a slight increase in arterial pH but did not affect cardiac output or cause pulmonary edema. We conclude that serotonin increases lung transvascular filtration primarily by increasing the transmural pressure gradient in exchanging vessels rather than by increasing vascular permeability."}
{"id": "PMID:1093751", "title": "Role of the collecting duct in the regulation of sodium balance.", "content": "A large body of evidence has accumulated which demonstrates that sodium transport in some distal nephron segment is altered in response to changes in extracellular fluid volume. Sodium reabsorption in the loop of Henle and distal tubule is directly related to delivery rate and is not inhibited by volume expansion. In contrast, recent studies have shown that Ringer loading causes a greater natriuretic response than hyperoncotic albumin because of diminished collecting duct sodium transport in the former model. Additional studies in animals with different basal extracellular fluid volumes and in DOCA-escape rats indicate further that the collecting duct is an important regulator of sodium balance. Although the factors that modulate sodium transport in the collecting duct are not clear, it is postulated that the local release of prostaglandins may be of major importance.", "contents": "Role of the collecting duct in the regulation of sodium balance. A large body of evidence has accumulated which demonstrates that sodium transport in some distal nephron segment is altered in response to changes in extracellular fluid volume. Sodium reabsorption in the loop of Henle and distal tubule is directly related to delivery rate and is not inhibited by volume expansion. In contrast, recent studies have shown that Ringer loading causes a greater natriuretic response than hyperoncotic albumin because of diminished collecting duct sodium transport in the former model. Additional studies in animals with different basal extracellular fluid volumes and in DOCA-escape rats indicate further that the collecting duct is an important regulator of sodium balance. Although the factors that modulate sodium transport in the collecting duct are not clear, it is postulated that the local release of prostaglandins may be of major importance."}
{"id": "PMID:1093752", "title": "Blood pressure regulation in end-stage renal disease and anephric man.", "content": "The hemodynamic pattern of response to bilateral nephrectomy was studied in 29 patients with end-stage renal disease on maintenance hemodialysis. Four patterns of hemodynamic response were seen. In 12 patients with nonmalignant hypertension, bilateral nephrectomy reduced blood pressure and total peripheral resistance with no effects on cardiac output. In 5 patients with malignant hypertension, bilateral nephrectomy reduced blood pressure, increased cardiac index, and reduced total peripheral resistance more markedly. In these two groups, at equivalent levels of total exchangeable sodium, before and after bilateral nephrectomy, mean arterial pressure and total peripheral resistance were invaribly lower in the absence of renal tissue. In 3 additional patients with nonmalignant hypertension, the decrease in blood pressure after bilateral nephrectomy was delayed from 3 to 12 weeks. When this occurred spontaneously, it was accompanied by a decrease in total peripheral resistance. The fourth hemodynamic pattern was seen in 6 normotensive patients with end-stage renal disease. After bilateral nephrectomy, there were no significant changes in mean arterial pressure, total peripheral resistance, or cardiac output. Salt and water loading failed to elevate blood pressure significantly. Renal transplantation was performed in 3 hypertensive patients before removal of the end-stage kidney. The functioning renal homograft did not result in normal blood pressure as long as the end-stage kidneys remained in place. Removal of the end-stage kidneys significantly decreased mean arterial pressure and total peripheral resistance. In the anephric state, a sharp difference was seen in blood pressure response to salt and water loading between previously normotensive and previously hypertensive patients. Previously hypertensive patients responded with a progressive increase in blood pressure that reached hypertensive levels. Previously normotensive patients failed to elevate their blood pressure significantly. It is concluded that the vasopressor function of the kidney is the most important factor in the pathophysiology of hypertension of end-state renal disease. Expansion of body fluid plays a role, but elevates the blood pressure only in patients who were previously hypertensive. The antihypertensive function of the kidney does not appear to be a major factor in the regulation of blood pressure in end-stage renal disease.", "contents": "Blood pressure regulation in end-stage renal disease and anephric man. The hemodynamic pattern of response to bilateral nephrectomy was studied in 29 patients with end-stage renal disease on maintenance hemodialysis. Four patterns of hemodynamic response were seen. In 12 patients with nonmalignant hypertension, bilateral nephrectomy reduced blood pressure and total peripheral resistance with no effects on cardiac output. In 5 patients with malignant hypertension, bilateral nephrectomy reduced blood pressure, increased cardiac index, and reduced total peripheral resistance more markedly. In these two groups, at equivalent levels of total exchangeable sodium, before and after bilateral nephrectomy, mean arterial pressure and total peripheral resistance were invaribly lower in the absence of renal tissue. In 3 additional patients with nonmalignant hypertension, the decrease in blood pressure after bilateral nephrectomy was delayed from 3 to 12 weeks. When this occurred spontaneously, it was accompanied by a decrease in total peripheral resistance. The fourth hemodynamic pattern was seen in 6 normotensive patients with end-stage renal disease. After bilateral nephrectomy, there were no significant changes in mean arterial pressure, total peripheral resistance, or cardiac output. Salt and water loading failed to elevate blood pressure significantly. Renal transplantation was performed in 3 hypertensive patients before removal of the end-stage kidney. The functioning renal homograft did not result in normal blood pressure as long as the end-stage kidneys remained in place. Removal of the end-stage kidneys significantly decreased mean arterial pressure and total peripheral resistance. In the anephric state, a sharp difference was seen in blood pressure response to salt and water loading between previously normotensive and previously hypertensive patients. Previously hypertensive patients responded with a progressive increase in blood pressure that reached hypertensive levels. Previously normotensive patients failed to elevate their blood pressure significantly. It is concluded that the vasopressor function of the kidney is the most important factor in the pathophysiology of hypertension of end-state renal disease. Expansion of body fluid plays a role, but elevates the blood pressure only in patients who were previously hypertensive. The antihypertensive function of the kidney does not appear to be a major factor in the regulation of blood pressure in end-stage renal disease."}
{"id": "PMID:1093753", "title": "Development and maintenance of renal hypertension in normal and guanethidine sympathectomized rats.", "content": "The sympathetic nervous system has been postulated to play a role in the maintenance of renal hypertension. Permanent peripheral sympathectomy was performed by treating newborn rats for 21 days with guanethidine. Sympathectomy was confirmed by (1) relative insensitivity to tyramine, (2) lack of responsiveness to renal nerve stimulation, and (3) absence of dopamine-beta-hydroxylase immunofluorescence in renal blood vessels. Placement of a clip on the left renal artery led to the development of two-kidney renal hypertension. No differences were observed between the two-kidney renal hypertensive normal and sympathectomized rats; both had elevated plasma renin activity and vasodepression with angiotensin antagonists which were maintained up to nine weeks. Furthermore, in normal rats chronic beta-adrenergic blockade with propranolol caused no change in the development of the two-kidney renal hypertension. Similarly, no differences were seen in blood pressure, plasma renin activity, or response to antagonists between the one-kidney renal hypertensive (clip plus contralateral nephrectomy) normal and sympathectomized rats. Both showed sustained low renin hypertension up to 12 weeks. The absence of the peripheral sympathetic nervous system did not affect the development or maintenance of hypertension in either model of hypertension.", "contents": "Development and maintenance of renal hypertension in normal and guanethidine sympathectomized rats. The sympathetic nervous system has been postulated to play a role in the maintenance of renal hypertension. Permanent peripheral sympathectomy was performed by treating newborn rats for 21 days with guanethidine. Sympathectomy was confirmed by (1) relative insensitivity to tyramine, (2) lack of responsiveness to renal nerve stimulation, and (3) absence of dopamine-beta-hydroxylase immunofluorescence in renal blood vessels. Placement of a clip on the left renal artery led to the development of two-kidney renal hypertension. No differences were observed between the two-kidney renal hypertensive normal and sympathectomized rats; both had elevated plasma renin activity and vasodepression with angiotensin antagonists which were maintained up to nine weeks. Furthermore, in normal rats chronic beta-adrenergic blockade with propranolol caused no change in the development of the two-kidney renal hypertension. Similarly, no differences were seen in blood pressure, plasma renin activity, or response to antagonists between the one-kidney renal hypertensive (clip plus contralateral nephrectomy) normal and sympathectomized rats. Both showed sustained low renin hypertension up to 12 weeks. The absence of the peripheral sympathetic nervous system did not affect the development or maintenance of hypertension in either model of hypertension."}
{"id": "PMID:1093754", "title": "Cardiovascular regulation by central adrenergic mechanisms and its alteration by hypotensive drugs.", "content": "Electrical stimulation of the posterior hypothalamus is followed by an immediate increase in sympathetic nerve activity and rise in blood pressure. Destruction of hypothalamic adrenergic structures by local unilateral injection of 6-hydroxydopamine into the posterior hypothalamus reduced the blood pressure rise in response to stimulation of the lesioned side. This and numerous other findings indicate an involvement of central adrenergic neurons in the mediation of an increase of sympathetic nerve activity caused by hypothalamic stimulation. However, central adrenergic neurons do not seem to be an integral part of the sympathoexcitatory pathways originating in the posterior hypothalamus but rather facilitate their activation: after almost complete norepinephrine depletion produced by combined treatment with reserpine and alpha-methl-p-tyrosine, hypothalamic stimulation was still followed by an increase in spontaneous sympathetic nerve activity. Stimulation of an alpha-adrenoceptive site, probably located in the lower brain stem, mimics an activation of the baroreceptor reflex. The hypotensive drug, clonidine, stimulates this alpha-adrenoceptive site. In low doses clonidine facilitates the activation of the reflex, and in high doses this drug induces a state which closely resembles a pronounced activation of the reflex. Experiments following depletion of norepinephrine suggest that the central part of the baroreceptor reflex arc does not contain adrenergic neurons. However, these findings are compatible with the view that some neurons within the reflex arc are supplied with alpha-adrenoceptors. For the present it cannot be stated with certainty whether these alpha-adrenoceptors possess an innervation by adrenergic neurons projecting onto the reflex arc. In favor of such an innervation are the obsevations that alpha-methyldopa has its site of action in the lower brain stem and that the integrity of central adrenergic neurons is essential for its hypotensive effect. It seems that two central adrenergic systems exist with opposing effects on cardiovascular control. These are an excitatory hypothalamic and an inhibitory bulbar adrenergic system. Partial destruction of central adrenergic neurons by intraventricularly injected 6-hydroxydopamine prevents the development of DOCA/NaCl, renal, and neurogenic hypertension and alters the pattern of blood pressure rise in spontaneously hypertensive rats. Impairment of central adrenergic function or imbalance of the two central adrenergic mechanisms may represent a trigger mechanism for the initiation of hypertension.", "contents": "Cardiovascular regulation by central adrenergic mechanisms and its alteration by hypotensive drugs. Electrical stimulation of the posterior hypothalamus is followed by an immediate increase in sympathetic nerve activity and rise in blood pressure. Destruction of hypothalamic adrenergic structures by local unilateral injection of 6-hydroxydopamine into the posterior hypothalamus reduced the blood pressure rise in response to stimulation of the lesioned side. This and numerous other findings indicate an involvement of central adrenergic neurons in the mediation of an increase of sympathetic nerve activity caused by hypothalamic stimulation. However, central adrenergic neurons do not seem to be an integral part of the sympathoexcitatory pathways originating in the posterior hypothalamus but rather facilitate their activation: after almost complete norepinephrine depletion produced by combined treatment with reserpine and alpha-methl-p-tyrosine, hypothalamic stimulation was still followed by an increase in spontaneous sympathetic nerve activity. Stimulation of an alpha-adrenoceptive site, probably located in the lower brain stem, mimics an activation of the baroreceptor reflex. The hypotensive drug, clonidine, stimulates this alpha-adrenoceptive site. In low doses clonidine facilitates the activation of the reflex, and in high doses this drug induces a state which closely resembles a pronounced activation of the reflex. Experiments following depletion of norepinephrine suggest that the central part of the baroreceptor reflex arc does not contain adrenergic neurons. However, these findings are compatible with the view that some neurons within the reflex arc are supplied with alpha-adrenoceptors. For the present it cannot be stated with certainty whether these alpha-adrenoceptors possess an innervation by adrenergic neurons projecting onto the reflex arc. In favor of such an innervation are the obsevations that alpha-methyldopa has its site of action in the lower brain stem and that the integrity of central adrenergic neurons is essential for its hypotensive effect. It seems that two central adrenergic systems exist with opposing effects on cardiovascular control. These are an excitatory hypothalamic and an inhibitory bulbar adrenergic system. Partial destruction of central adrenergic neurons by intraventricularly injected 6-hydroxydopamine prevents the development of DOCA/NaCl, renal, and neurogenic hypertension and alters the pattern of blood pressure rise in spontaneously hypertensive rats. Impairment of central adrenergic function or imbalance of the two central adrenergic mechanisms may represent a trigger mechanism for the initiation of hypertension."}
{"id": "PMID:1093756", "title": "Effect of salt depletion and propranolol on blood pressure and plasma renin activity in various forms of hypertension.", "content": "The effect of propranolol therapy on the mean arterial pressure (MAP) and plasma renin activity (PRA) was studied in three groups of hypertensive patients who were also treated with saliuretics. Group A: In 14 patients with essential hypertension on chlorthalidone treatment, an additional daily dose of 640 mg propranolol for two months led to a significant reduction of the MAP (from 124 to 105 mm Hg) and PRA (from 5.3 to 2.0 ng AI/ml/hr standing). There was no correlation between MAP reduction and either the original levels or change in PRA. Group B: In 14 patients with essential hypertension and 5 with renal artery stenosis studied on a fixed salt intake, the plasma and extracellular volumes, PRA, and blood pressures were recorded before and after three days of diuretic induced salt depletion and, with maintenance of the depleted state, after three days of propranolol. Salt depletion resulted in a decrease in MAP from 132 to 128 mm Hg (NS), and PRA increased from 3.4 to 22.3 ng AI/ml/hr (P less than 0.01). There was no correlation between change in MAP and PRA control values, PRA change, or any of the volume parameters. Addition of propranolol was followed by a rapid MAP decrease to 111 mm Hg (P less than 0.01), and the PRA dropped to a mean of 8.5 (P less than 0.01). No correlation was found between change in MAP and change in PRA. The patients with renal artery stenosis did not differ in their reactions from those with essential hypertension. Group C: In five patients with moderate renal failure and normal to expanded 82-Br distribution volume, propranolol lowered MAP by 10% and lowered the PRA in all five. Salt depletion by furosemide to 82-Br volumes below normal resulted in a 10% decrease of MAP and a marked rise in PRA. In this state propranolol was followed by a further MAP reduction of 18% and a decrease in PRA. There was no quantitative relationship between MAP and PRA change during either of the treatment regimes. It is concluded that in various forms of hypertension, the blood pressure can be effectively lowered by combining diuretics and propranolol regardless of the pretreatment PRA level.", "contents": "Effect of salt depletion and propranolol on blood pressure and plasma renin activity in various forms of hypertension. The effect of propranolol therapy on the mean arterial pressure (MAP) and plasma renin activity (PRA) was studied in three groups of hypertensive patients who were also treated with saliuretics. Group A: In 14 patients with essential hypertension on chlorthalidone treatment, an additional daily dose of 640 mg propranolol for two months led to a significant reduction of the MAP (from 124 to 105 mm Hg) and PRA (from 5.3 to 2.0 ng AI/ml/hr standing). There was no correlation between MAP reduction and either the original levels or change in PRA. Group B: In 14 patients with essential hypertension and 5 with renal artery stenosis studied on a fixed salt intake, the plasma and extracellular volumes, PRA, and blood pressures were recorded before and after three days of diuretic induced salt depletion and, with maintenance of the depleted state, after three days of propranolol. Salt depletion resulted in a decrease in MAP from 132 to 128 mm Hg (NS), and PRA increased from 3.4 to 22.3 ng AI/ml/hr (P less than 0.01). There was no correlation between change in MAP and PRA control values, PRA change, or any of the volume parameters. Addition of propranolol was followed by a rapid MAP decrease to 111 mm Hg (P less than 0.01), and the PRA dropped to a mean of 8.5 (P less than 0.01). No correlation was found between change in MAP and change in PRA. The patients with renal artery stenosis did not differ in their reactions from those with essential hypertension. Group C: In five patients with moderate renal failure and normal to expanded 82-Br distribution volume, propranolol lowered MAP by 10% and lowered the PRA in all five. Salt depletion by furosemide to 82-Br volumes below normal resulted in a 10% decrease of MAP and a marked rise in PRA. In this state propranolol was followed by a further MAP reduction of 18% and a decrease in PRA. There was no quantitative relationship between MAP and PRA change during either of the treatment regimes. It is concluded that in various forms of hypertension, the blood pressure can be effectively lowered by combining diuretics and propranolol regardless of the pretreatment PRA level."}
{"id": "PMID:1093755", "title": "Pharmacological effects of alpha-methyldopa, alpha-methylnorepinephrine, and octopamine on rat arteriolar, arterial, and terminal vascular smooth.", "content": "Experiments on rat mesenteric arterioles, metarterioles, and aortae demonstrate that although alpha-methylnorepinephrine is much less potent in inducing contraction than epinephrine on all three blood vessel types, it is either equivalent or only one and a half to two times less potent than the natural postganglionic neurotransmitter, norepinephrine, on these blood vessels. Furthermore, alpha-methylnorepinephrine is equivalent to norepinephrine in its ability to induce maximal contractile responses on rat arterioles, metarterioles, and aortae. Systemic administration of alpha-methyldopa to rats for 15 days shifted the log dose-response curves for all three catecholamines, but not vasopressin or potassium chloride, to the right of all three blood vessel types; the maximal contractile responses to these amines were, however, not affected by chronic treatment with alpha-methyldopa. In addition, acute, intra-arterial administration of 500 mg/kg of alpha-methyldopa was found not only to induce mesenteric arteriolar vasodilatation gradually but also to depress arteriolar reponsiveness to catecholamines. In view of these direct findings, it is difficult to accept the hypothesis that alpha-methyldopa induces hypotension via formation of a \"false\" postganglionic neurotransmitter substance, namely, alpha-methylnorepinephrine. The present findings suggest that alpha-methyldopa may exert some of its anti-hypertensive action, at least in the rat, by (1) depressing arteriolar responsiveness to circulating and released catecholamines and (2) some unknown direct action on peripheral vascular muscle. In addition, the present study indicates that octopamine is (1) between 60 and 15,000 times less potent than norepinephrine on rat arterioles and metarterioles and (2) incapable of eliciting more than a 40% occlusion of these terminal vessels. It is suggested that such data support the concept that octopamine, in contrast to alpha-methylnorepinephrine, could serve as a false adrenergic neurotransmitter agent and thus account for part or all of the hypotensive action of monoamine oxidase inhibitors like pargyline. The use of complete dose-response curves, several different adrenergic compounds (i.e., epinephrine, norepinephrine, alpha-methylnorepinephrine, octopamine, phenylephrine, and dopamine), and different rat blood vessels supports the concept that adrenergic molecules containing a catecholamine nucleus and a beta-hydroxyl group elicit the most potent constrictor responses from peripheral blood vessels. In addition, the data suggest that the structure-activity relationships for catecholamines and their analogs on terminal vascular smooth muscle are probably different from those for arterial smooth muscle.", "contents": "Pharmacological effects of alpha-methyldopa, alpha-methylnorepinephrine, and octopamine on rat arteriolar, arterial, and terminal vascular smooth. Experiments on rat mesenteric arterioles, metarterioles, and aortae demonstrate that although alpha-methylnorepinephrine is much less potent in inducing contraction than epinephrine on all three blood vessel types, it is either equivalent or only one and a half to two times less potent than the natural postganglionic neurotransmitter, norepinephrine, on these blood vessels. Furthermore, alpha-methylnorepinephrine is equivalent to norepinephrine in its ability to induce maximal contractile responses on rat arterioles, metarterioles, and aortae. Systemic administration of alpha-methyldopa to rats for 15 days shifted the log dose-response curves for all three catecholamines, but not vasopressin or potassium chloride, to the right of all three blood vessel types; the maximal contractile responses to these amines were, however, not affected by chronic treatment with alpha-methyldopa. In addition, acute, intra-arterial administration of 500 mg/kg of alpha-methyldopa was found not only to induce mesenteric arteriolar vasodilatation gradually but also to depress arteriolar reponsiveness to catecholamines. In view of these direct findings, it is difficult to accept the hypothesis that alpha-methyldopa induces hypotension via formation of a \"false\" postganglionic neurotransmitter substance, namely, alpha-methylnorepinephrine. The present findings suggest that alpha-methyldopa may exert some of its anti-hypertensive action, at least in the rat, by (1) depressing arteriolar responsiveness to circulating and released catecholamines and (2) some unknown direct action on peripheral vascular muscle. In addition, the present study indicates that octopamine is (1) between 60 and 15,000 times less potent than norepinephrine on rat arterioles and metarterioles and (2) incapable of eliciting more than a 40% occlusion of these terminal vessels. It is suggested that such data support the concept that octopamine, in contrast to alpha-methylnorepinephrine, could serve as a false adrenergic neurotransmitter agent and thus account for part or all of the hypotensive action of monoamine oxidase inhibitors like pargyline. The use of complete dose-response curves, several different adrenergic compounds (i.e., epinephrine, norepinephrine, alpha-methylnorepinephrine, octopamine, phenylephrine, and dopamine), and different rat blood vessels supports the concept that adrenergic molecules containing a catecholamine nucleus and a beta-hydroxyl group elicit the most potent constrictor responses from peripheral blood vessels. In addition, the data suggest that the structure-activity relationships for catecholamines and their analogs on terminal vascular smooth muscle are probably different from those for arterial smooth muscle."}
{"id": "PMID:1093757", "title": "Partial versus complete control of blood pressure in the prevention of hypertensive complications.", "content": "The data from the Veterans Administration Cooperative Study on Antihypertensive Agents Prevention of Morbidity Trial were reviewed from the point of view of the importance of normalizing the blood pressure in preventing cardiovascular complications. The treated group of patients was subdivided into three subgroups on the basis of the diastolic blood pressures recorded at the fourth month after randomization as follows: subgroup A, the poorly controlled patients whose diastolic blood pressures were 90 mm Hg or higher, an intermediate subgroup B with diatolic levels of 81 to 89 mm Hg, and subgroup C, the best controlled patients, all of whom exhibited diastolic blood pressures of 80 mm Hg or less. While the incidence of morbid events was slightly higher in subgroup A than in the other subgroups, the differences were not statistically significant. On the other hand, the incidence of morbid events in subgroup A was significantly lower (P less than 0.05) than in the untreated control patients (14.9% in subgroup A vs. 28.9% in the control group). While normalization of blood pressure is a desirable goal of treatment, even partial reduction appears to exert significant therapeutic benefit.", "contents": "Partial versus complete control of blood pressure in the prevention of hypertensive complications. The data from the Veterans Administration Cooperative Study on Antihypertensive Agents Prevention of Morbidity Trial were reviewed from the point of view of the importance of normalizing the blood pressure in preventing cardiovascular complications. The treated group of patients was subdivided into three subgroups on the basis of the diastolic blood pressures recorded at the fourth month after randomization as follows: subgroup A, the poorly controlled patients whose diastolic blood pressures were 90 mm Hg or higher, an intermediate subgroup B with diatolic levels of 81 to 89 mm Hg, and subgroup C, the best controlled patients, all of whom exhibited diastolic blood pressures of 80 mm Hg or less. While the incidence of morbid events was slightly higher in subgroup A than in the other subgroups, the differences were not statistically significant. On the other hand, the incidence of morbid events in subgroup A was significantly lower (P less than 0.05) than in the untreated control patients (14.9% in subgroup A vs. 28.9% in the control group). While normalization of blood pressure is a desirable goal of treatment, even partial reduction appears to exert significant therapeutic benefit."}
{"id": "PMID:1093758", "title": "Return of elevated blood pressure after withdrawal of antihypertensive drugs.", "content": "The rate at which arterial pressure rises after discontinuing active treatment was investigated in a group of 86 hypertensive patients who received treatment with hydrochlorothiazide, reserpine and hydralazine for two years or longer and whose diastolic pressures averaged below 96 mm Hg for the last year of treatment. Sixty patients were assigned double-blind to placebos and 26 were continued on active drugs. Forty-two of the placebo group of patients were removed over an 18 month follow-up because of return of elevated blood pressures, 39 being removed in the first six months. Six patients in the placebo group and none in the treated group were removed because of morbid events. Nine or 15% of the placebo patients remained normotensive. The rate of rise in arterial pressure in the placebo group appeared to be related directly to the height of the pressure prior to initiation of active treatment and inversely to the age of the patients. Serum uric acid fell significantly while serum potassium rose significantly after active treatment was discontinued. The glucose tolerance test changed slightly in a direction toward normal while serum creatinine showed no significant change.", "contents": "Return of elevated blood pressure after withdrawal of antihypertensive drugs. The rate at which arterial pressure rises after discontinuing active treatment was investigated in a group of 86 hypertensive patients who received treatment with hydrochlorothiazide, reserpine and hydralazine for two years or longer and whose diastolic pressures averaged below 96 mm Hg for the last year of treatment. Sixty patients were assigned double-blind to placebos and 26 were continued on active drugs. Forty-two of the placebo group of patients were removed over an 18 month follow-up because of return of elevated blood pressures, 39 being removed in the first six months. Six patients in the placebo group and none in the treated group were removed because of morbid events. Nine or 15% of the placebo patients remained normotensive. The rate of rise in arterial pressure in the placebo group appeared to be related directly to the height of the pressure prior to initiation of active treatment and inversely to the age of the patients. Serum uric acid fell significantly while serum potassium rose significantly after active treatment was discontinued. The glucose tolerance test changed slightly in a direction toward normal while serum creatinine showed no significant change."}
{"id": "PMID:1093759", "title": "Radionuclide angiocardiography. Improved diagnosis and quantitation of left-to-right shunts using area ratio techniques in children.", "content": "A comparison of several reported methods for detection and quantitation of left-to-right shunts by radionuclides was performed in 50 children. Count ratio (C2/C1) techniques were compared with the exponential extrapolation and gamma function area ratio techniques. C2/C1 ratios accurately detected shunts and could reliably separate shunts from normals, but there was a high rate of false positives in children with valvular heart disease. The area ratio methods provided more accurate shunt quantitation and a better separation of patients with valvular heart disease than did the C2/C1 ratio. The gamma function method showed a higher correlation with oximetry than the exponential method, but the difference was not statistically significant. For accurate shunt quantitation and a reliable separation of patients with valvular heart disease from those with shunts, area ratio calculations are preferable to the C2/C1 ratio.", "contents": "Radionuclide angiocardiography. Improved diagnosis and quantitation of left-to-right shunts using area ratio techniques in children. A comparison of several reported methods for detection and quantitation of left-to-right shunts by radionuclides was performed in 50 children. Count ratio (C2/C1) techniques were compared with the exponential extrapolation and gamma function area ratio techniques. C2/C1 ratios accurately detected shunts and could reliably separate shunts from normals, but there was a high rate of false positives in children with valvular heart disease. The area ratio methods provided more accurate shunt quantitation and a better separation of patients with valvular heart disease than did the C2/C1 ratio. The gamma function method showed a higher correlation with oximetry than the exponential method, but the difference was not statistically significant. For accurate shunt quantitation and a reliable separation of patients with valvular heart disease from those with shunts, area ratio calculations are preferable to the C2/C1 ratio."}
{"id": "PMID:1093762", "title": "Diagnostic techniques: closed biopsy of bone.", "content": "Closed biopsy of bone has proven to be a useful and accurate method of establishing the diagnosis of certain primary and secondary bone tumors, infections, and metabolic disorders. The use of a Craig needle for biopsy has simplified this technique and has provided the orthopedic surgeon with an excellent diagnostic tool.", "contents": "Diagnostic techniques: closed biopsy of bone. Closed biopsy of bone has proven to be a useful and accurate method of establishing the diagnosis of certain primary and secondary bone tumors, infections, and metabolic disorders. The use of a Craig needle for biopsy has simplified this technique and has provided the orthopedic surgeon with an excellent diagnostic tool."}
{"id": "PMID:1093763", "title": "Bone scanning: principles, technique and interpretation.", "content": "Bone scanning is most useful in the detection of bone metastases. The recent introduction of new radiopharmaceuticals and instrumentation has reduced the time needed to perform the study and its relative cost, while increasing the usefulness of the study in detecting roentgenographically occult diseases. Metastatic disease is used as the pathophysiologic model for understanding the principles of bone scanning. When a tumor invades bone, in addition to causing bone destruction, it also causes reactive bone formation or repair. It is here that radioisotopes are of considerable value, since some radionuclides are incorporated into the hydroxyapatite crystals of reactive bone. Bone repair is described as occurring in three phases. In Phase I, the roentgenogram shows no change in bone density, but the scan is abnormal. In Phase II, both scintigraphic and roentgenographic abnormalities increase, and in Phase III, when the osteoid has calcified completely, the roentgenogram shows radiodensities and the scan appears almost normal. Fewer than 5 per cent of patients have a normal scan in the presence of an abnormal roentgenogram. Presently, most bone scans are performed with phosphate compounds labeled with -99m-Tc. In the past, 85-Sr, 87M-Sr, and 18-F were more broadly used. Scanning may be performed on either a rectilinear scanner or a scintophoto (gamma) camera. Areas which are abnormal on bone scan should be interpreted with current roentgenograms in the light of clinical findings.", "contents": "Bone scanning: principles, technique and interpretation. Bone scanning is most useful in the detection of bone metastases. The recent introduction of new radiopharmaceuticals and instrumentation has reduced the time needed to perform the study and its relative cost, while increasing the usefulness of the study in detecting roentgenographically occult diseases. Metastatic disease is used as the pathophysiologic model for understanding the principles of bone scanning. When a tumor invades bone, in addition to causing bone destruction, it also causes reactive bone formation or repair. It is here that radioisotopes are of considerable value, since some radionuclides are incorporated into the hydroxyapatite crystals of reactive bone. Bone repair is described as occurring in three phases. In Phase I, the roentgenogram shows no change in bone density, but the scan is abnormal. In Phase II, both scintigraphic and roentgenographic abnormalities increase, and in Phase III, when the osteoid has calcified completely, the roentgenogram shows radiodensities and the scan appears almost normal. Fewer than 5 per cent of patients have a normal scan in the presence of an abnormal roentgenogram. Presently, most bone scans are performed with phosphate compounds labeled with -99m-Tc. In the past, 85-Sr, 87M-Sr, and 18-F were more broadly used. Scanning may be performed on either a rectilinear scanner or a scintophoto (gamma) camera. Areas which are abnormal on bone scan should be interpreted with current roentgenograms in the light of clinical findings."}
{"id": "PMID:1093764", "title": "The detection of venous thrombosis.", "content": "It is imperative that the orthopedic surgeon recognize the magnitude of the problem of thromboembolic disease. It is the most common complication of trauma or surgical procedures. Because the clinical signs and symptoms of deep venous thrombosis are so variable, and since pulmonary embolism is often the first sign of deep venous thrombosis, we must continue to refine our techniques for screening and diagnosis. The 125-I fibrinogen localization of propagating venous thrombi in the legs is a very promising and sensitive screening procedure. With refinements, the Doppler effect flowmeter and impedance phlebography may prove to be valuable screening techniques. Venography is still one of the most effective procedures in detecting venous thrombi and remains the standard of detection. The search must continue for better methods of prevention, diagnosis, and treatment.", "contents": "The detection of venous thrombosis. It is imperative that the orthopedic surgeon recognize the magnitude of the problem of thromboembolic disease. It is the most common complication of trauma or surgical procedures. Because the clinical signs and symptoms of deep venous thrombosis are so variable, and since pulmonary embolism is often the first sign of deep venous thrombosis, we must continue to refine our techniques for screening and diagnosis. The 125-I fibrinogen localization of propagating venous thrombi in the legs is a very promising and sensitive screening procedure. With refinements, the Doppler effect flowmeter and impedance phlebography may prove to be valuable screening techniques. Venography is still one of the most effective procedures in detecting venous thrombi and remains the standard of detection. The search must continue for better methods of prevention, diagnosis, and treatment."}
{"id": "PMID:1093765", "title": "Minimal internal fixation of tibial fractures.", "content": "Flexible wire and small pins cause minimal disturbance of osseous blood supply, and introduce minimal foreign material into the wound. Supplemental support by a plaster cast or by traction is required, but the external support can generally be discontinued  early for joint mobilization. Several simple auxillary fixation devices extend the usefulness of wire fixation. Removal of metal is not required. Many common fractures of the tibia are amenable to this method of minimal internal fixation. In the diaphysis, long oblique fractures are the most suitable for this application; the firmness of their fixation by cerclage is augmented by muscle pull. Rotation is effectively controlled by a plate which is L-shaped in cross section, and is held in position by cerclage. In the metaphysis, articular fractures of the knee and ankle are securely fixed by a flattened loop of wire and two washers (wire-washer set), supplemented sometimes by pins or hand-made staples. Two pins alone provide excellent fixation of the medial malleolus. A single pin, or a single wire loop through drill holes, may be sufficient to impart stability to an unstable tibial fracture. A key-type graft of iliac bone, maintained by crossed wire loops through cortical drill holes, is effective in the tibial diaphysis. Autogenous iliac cancellous chips provide minimal and effective internal fixation for an infected ununited fracture of the tibia. The surgical instrument most important for making wire fixation highly successful is a tightener-twister which protects wire loops from excessive strain during application, and permits twisting at a predetermined and therefore reproducible tension. Other special and ordinary instruments are valuable assets.", "contents": "Minimal internal fixation of tibial fractures. Flexible wire and small pins cause minimal disturbance of osseous blood supply, and introduce minimal foreign material into the wound. Supplemental support by a plaster cast or by traction is required, but the external support can generally be discontinued  early for joint mobilization. Several simple auxillary fixation devices extend the usefulness of wire fixation. Removal of metal is not required. Many common fractures of the tibia are amenable to this method of minimal internal fixation. In the diaphysis, long oblique fractures are the most suitable for this application; the firmness of their fixation by cerclage is augmented by muscle pull. Rotation is effectively controlled by a plate which is L-shaped in cross section, and is held in position by cerclage. In the metaphysis, articular fractures of the knee and ankle are securely fixed by a flattened loop of wire and two washers (wire-washer set), supplemented sometimes by pins or hand-made staples. Two pins alone provide excellent fixation of the medial malleolus. A single pin, or a single wire loop through drill holes, may be sufficient to impart stability to an unstable tibial fracture. A key-type graft of iliac bone, maintained by crossed wire loops through cortical drill holes, is effective in the tibial diaphysis. Autogenous iliac cancellous chips provide minimal and effective internal fixation for an infected ununited fracture of the tibia. The surgical instrument most important for making wire fixation highly successful is a tightener-twister which protects wire loops from excessive strain during application, and permits twisting at a predetermined and therefore reproducible tension. Other special and ordinary instruments are valuable assets."}
{"id": "PMID:1093766", "title": "Electrodiagnosis in orthopedic surgery.", "content": "The electromyogram is gaining importance as a diagnostic tool in the clinical practice of orthopedic surgery. Its uses in the area of kinesiology will undoubtedly be further developed in the future in ways which can be standardized and made available to the practicing orthopedist to improve the results of muscle and tendon surgery and the design of surgical procedures. It should be noted that in either case electromyograms can be utilized to give a great deal of valuable information concerning the competence of the motor unit, the functioning mass of muscle fibers and the phasic relationships of muscle groups. Its value in diagnosis, however, depends directly upon the care with which it is performed, the patience of the electromyographer in sampling an adequate quantity of muscle mass to be certain that nothing has been overlooked and, above all, the specificity of the orthopedic surgeon in delineating exactly what information he wishes to obtain, so that the performance of the electromyogram can be confirmed and aimed directly at producing answers to the questions he is asking. In this regard, the electromyogram is in no sense a screening test or a catchall which the orthopedic surgeon can substitute for a careful clinical evaluation.", "contents": "Electrodiagnosis in orthopedic surgery. The electromyogram is gaining importance as a diagnostic tool in the clinical practice of orthopedic surgery. Its uses in the area of kinesiology will undoubtedly be further developed in the future in ways which can be standardized and made available to the practicing orthopedist to improve the results of muscle and tendon surgery and the design of surgical procedures. It should be noted that in either case electromyograms can be utilized to give a great deal of valuable information concerning the competence of the motor unit, the functioning mass of muscle fibers and the phasic relationships of muscle groups. Its value in diagnosis, however, depends directly upon the care with which it is performed, the patience of the electromyographer in sampling an adequate quantity of muscle mass to be certain that nothing has been overlooked and, above all, the specificity of the orthopedic surgeon in delineating exactly what information he wishes to obtain, so that the performance of the electromyogram can be confirmed and aimed directly at producing answers to the questions he is asking. In this regard, the electromyogram is in no sense a screening test or a catchall which the orthopedic surgeon can substitute for a careful clinical evaluation."}
{"id": "PMID:1093768", "title": "Fractures of tibial plateaus.  A review of the literature.", "content": "Tibial plateau fractures, common injuries in the middle-aged, often result from falls or vehicular accidents.  The wide spectrum of fracture patterns can be theoretically related to the sequential application of bending (valgus/varus) and compression forces.  End-results of 599 fractures in 13 different studies were compared by fracture pattern using a clear-cut fracture classification.  Minimally displaced (less than 5-10 mm displacement) fractures average 85-90 per cent acceptable end-results.  Associated soft tissue lesions include frequent meniscal (50% of cases) and ligamentous (10-30%) injuries.  Vascular and nerve injuries are most unusual: non-union is not reported.  A diagnostic and therapeutic approach is outlined.  The assessment of bony and/or ligamentous instability is stressed.  Reduction is required in most displaced fractures: open reduction and rigid internal fixation with ample bone grafting is suggested in displaced local depression fractures and other unstable fracture types refractory to closed reduction.  Operative repair of ruptured ligaments seems indicated.  Early knee motion is important in all fractures.  In unstable fractures angular alignment must be maintained (rigid internal fixation, skeletal traction, or bracing) during early motion.  Quadriceps rehabilitation must be vigorous.  Weight-bearing should be deferred for at least three months.  The cast brace is useful in unicondylar fractures.", "contents": "Fractures of tibial plateaus.  A review of the literature. Tibial plateau fractures, common injuries in the middle-aged, often result from falls or vehicular accidents.  The wide spectrum of fracture patterns can be theoretically related to the sequential application of bending (valgus/varus) and compression forces.  End-results of 599 fractures in 13 different studies were compared by fracture pattern using a clear-cut fracture classification.  Minimally displaced (less than 5-10 mm displacement) fractures average 85-90 per cent acceptable end-results.  Associated soft tissue lesions include frequent meniscal (50% of cases) and ligamentous (10-30%) injuries.  Vascular and nerve injuries are most unusual: non-union is not reported.  A diagnostic and therapeutic approach is outlined.  The assessment of bony and/or ligamentous instability is stressed.  Reduction is required in most displaced fractures: open reduction and rigid internal fixation with ample bone grafting is suggested in displaced local depression fractures and other unstable fracture types refractory to closed reduction.  Operative repair of ruptured ligaments seems indicated.  Early knee motion is important in all fractures.  In unstable fractures angular alignment must be maintained (rigid internal fixation, skeletal traction, or bracing) during early motion.  Quadriceps rehabilitation must be vigorous.  Weight-bearing should be deferred for at least three months.  The cast brace is useful in unicondylar fractures."}
{"id": "PMID:1093787", "title": "Pathologic-anatomical findings and cerebral localization in stereotactic treatment of extrapyramidal motor disturbances in multiple sclerosis.", "content": "Two postmortem case of multiple sclerosis treated by sterotactic operations for the intention shaking of limbs, trunk, and head, and for the action myoclonus are analyzed to determine the location of the substrate of myoclonic and ballistic movements, the location of the coagulations for relief of these movements, and whether fresh demyelinating foci are elicited by intracerebral interventions. In the first case of a clinically typical multiple sclerosis, the foci responsible for the severe action myoclonus and intention ataxia of the trunk are demyelinations in the right and left red nucleus resulting in nerve cell damage and loss and an almost complete destruction of myelinated fibers. The restricted foci in the white matter of the cerebellum which do not involve the cerebellar nuclei are not extensive enough or old enough to be the cause of the action myoclonus but may, perhaps, sustain the pathogenesis. - In the second case of cerebral palsy and combined multiple sclerosis (detected post mortem), the combination of the severe damage of putamen and caudate nucleus by status marmoratus and the extensive nerve cell and fiber damage due to demyelinating foci in the substantia nigra are probably the substrate of the jactitation and intention myoclonus of the left limbs. The stereotactic coagulation of the dentatothalamic and pallidothalamic fibers in the base of V. o.p. and V.o.a. at the point where they pass through the zona incerta (location confirmed post mortem) resulted in a nearly complete relief of hyperkinetic movements. In the first case, fresh demyelinating foci are present in both hemispheres with stereotactic interventions; these foci are located, amongother places, around the coagulation and the electrode track. In the second case, post mortem serial brain sections demonstrate that stereotactic operations even in subacute multiple sclerosis can be carried out without eliciting any exacerbation of demyelination foci. Therefore, the danger exists that stereotactic intervention in cases of multiple sclerosis may precipitate fresh demyelinating foci. As our clinical experience [Riechert and Richter, 1972a, b] indicates, however, this occurred in markedly less than 10% of the cases.", "contents": "Pathologic-anatomical findings and cerebral localization in stereotactic treatment of extrapyramidal motor disturbances in multiple sclerosis. Two postmortem case of multiple sclerosis treated by sterotactic operations for the intention shaking of limbs, trunk, and head, and for the action myoclonus are analyzed to determine the location of the substrate of myoclonic and ballistic movements, the location of the coagulations for relief of these movements, and whether fresh demyelinating foci are elicited by intracerebral interventions. In the first case of a clinically typical multiple sclerosis, the foci responsible for the severe action myoclonus and intention ataxia of the trunk are demyelinations in the right and left red nucleus resulting in nerve cell damage and loss and an almost complete destruction of myelinated fibers. The restricted foci in the white matter of the cerebellum which do not involve the cerebellar nuclei are not extensive enough or old enough to be the cause of the action myoclonus but may, perhaps, sustain the pathogenesis. - In the second case of cerebral palsy and combined multiple sclerosis (detected post mortem), the combination of the severe damage of putamen and caudate nucleus by status marmoratus and the extensive nerve cell and fiber damage due to demyelinating foci in the substantia nigra are probably the substrate of the jactitation and intention myoclonus of the left limbs. The stereotactic coagulation of the dentatothalamic and pallidothalamic fibers in the base of V. o.p. and V.o.a. at the point where they pass through the zona incerta (location confirmed post mortem) resulted in a nearly complete relief of hyperkinetic movements. In the first case, fresh demyelinating foci are present in both hemispheres with stereotactic interventions; these foci are located, amongother places, around the coagulation and the electrode track. In the second case, post mortem serial brain sections demonstrate that stereotactic operations even in subacute multiple sclerosis can be carried out without eliciting any exacerbation of demyelination foci. Therefore, the danger exists that stereotactic intervention in cases of multiple sclerosis may precipitate fresh demyelinating foci. As our clinical experience [Riechert and Richter, 1972a, b] indicates, however, this occurred in markedly less than 10% of the cases."}
{"id": "PMID:1093805", "title": "Permeability changes in the blood-brain barrier: causes and consequences.", "content": "1. Generalized changes in blood-brain barrier (BBB) permeability are accompanied by extravasation of plasma proteins; thus, they are readily studied with protein markers or protein-dye complexes. Selective changes in permeability involve alterations in BBB transport systems; they are best studied with techniques which detect the qualitative hallmarks of carrier-mediated transport, namely saturation, competition, and stereospecificity. 2. Quantitative assessments of the selective permeability of the BBB can be made from the saturation data expressed in terms of Michaelis-Menten kinetics. The advantages of the latter are twofold: (a) alterations elicited by modified barrier affinity (Km) can be distinguished from alterations in carrier capacity (Vmax); (b) the relative rates of flux of a metabolite across the BBB can be placed in the perspective of cerebral metabolism. Kinetic data on transport processes in the BBB are obtained by either constant infusion or single injection techniques. Results obtained with both methodologies have been comparable. 3. Independent transport systems for glucose, neutral amino acids, basic amino acids, and monocarboxylic acids have been identified in the BBB. The description of these transport systems in kinetic terms provides a background of information on intact mechanisms to which altered transport can be compared. 4. Experimental evidence indicates that the availability of key metabolic substrates, such as glucose or essential amino acids, may be rate-limiting in cerebral metabolism. A working hypothesis was developed that the consequences of a selective change in barrier permeability to one or more of these essential substrates are directly related to altered rates of reaction in substrate-limited pathways, e.g., cerebral protein or neuro-transmitter biosynthesis. 5. Toxicological causes of generalized changes in BBB permeability include hypertonic solutions, organic solvents, surface-active agents, enzymes, and heavy metals. Some agents, e.g., mercury or hypertonic urea, induce selective changes in BBB transport at doses much lower than those required for nonspecific barrier break-down. Subtle changes in transport of metabolic substrates may remain unrecognized unless specifically investigated, yet may have profound consequences on brain metabolism. 6. Pathological processes can also induce selective changes in BBB permeability. Such changes often temporally precede the more generalized alterations in permeability that can occur during pathogenesis. For example, in brain edema due to an ischemic infarct, glucose transport increases during the early cytotoxic phase, whereas generalized changes are not detected until the later vasogenic phase.", "contents": "Permeability changes in the blood-brain barrier: causes and consequences. 1. Generalized changes in blood-brain barrier (BBB) permeability are accompanied by extravasation of plasma proteins; thus, they are readily studied with protein markers or protein-dye complexes. Selective changes in permeability involve alterations in BBB transport systems; they are best studied with techniques which detect the qualitative hallmarks of carrier-mediated transport, namely saturation, competition, and stereospecificity. 2. Quantitative assessments of the selective permeability of the BBB can be made from the saturation data expressed in terms of Michaelis-Menten kinetics. The advantages of the latter are twofold: (a) alterations elicited by modified barrier affinity (Km) can be distinguished from alterations in carrier capacity (Vmax); (b) the relative rates of flux of a metabolite across the BBB can be placed in the perspective of cerebral metabolism. Kinetic data on transport processes in the BBB are obtained by either constant infusion or single injection techniques. Results obtained with both methodologies have been comparable. 3. Independent transport systems for glucose, neutral amino acids, basic amino acids, and monocarboxylic acids have been identified in the BBB. The description of these transport systems in kinetic terms provides a background of information on intact mechanisms to which altered transport can be compared. 4. Experimental evidence indicates that the availability of key metabolic substrates, such as glucose or essential amino acids, may be rate-limiting in cerebral metabolism. A working hypothesis was developed that the consequences of a selective change in barrier permeability to one or more of these essential substrates are directly related to altered rates of reaction in substrate-limited pathways, e.g., cerebral protein or neuro-transmitter biosynthesis. 5. Toxicological causes of generalized changes in BBB permeability include hypertonic solutions, organic solvents, surface-active agents, enzymes, and heavy metals. Some agents, e.g., mercury or hypertonic urea, induce selective changes in BBB transport at doses much lower than those required for nonspecific barrier break-down. Subtle changes in transport of metabolic substrates may remain unrecognized unless specifically investigated, yet may have profound consequences on brain metabolism. 6. Pathological processes can also induce selective changes in BBB permeability. Such changes often temporally precede the more generalized alterations in permeability that can occur during pathogenesis. For example, in brain edema due to an ischemic infarct, glucose transport increases during the early cytotoxic phase, whereas generalized changes are not detected until the later vasogenic phase."}
{"id": "PMID:1093816", "title": "X inactivation, differentiation, and DNA methylation.", "content": "A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation. A key feature of the model is the proposal of sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites. Although such enzymes have not yet been detected in eukaryotes, they are known in bacteria. An argument is presented, based on recent data on DNA-binding proteins, that DNA methylation should affect the binding of regulatory proteins. In support of the model, short reviews are included covering both mammalian X inactivation and bacterial restriction and modification enzymes.", "contents": "X inactivation, differentiation, and DNA methylation. A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation. A key feature of the model is the proposal of sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites. Although such enzymes have not yet been detected in eukaryotes, they are known in bacteria. An argument is presented, based on recent data on DNA-binding proteins, that DNA methylation should affect the binding of regulatory proteins. In support of the model, short reviews are included covering both mammalian X inactivation and bacterial restriction and modification enzymes."}
{"id": "PMID:1093824", "title": "The synaptonemal complex and the spindle plaque during meiosis in yeast.", "content": "Meiosis in Saccharomyces cerevisiae proceeds principally in the same manner as in other Ascomycetes. Leptotene is characterized by unpaired lateral components and pachytene by the presence of extensive synaptonemal complexes. The synaptonemal complex has the same dimensions and is similar in structure to those described for other organisms. Chromosome counts can now be made by reconstructing the synaptonemal complexes. Diplotene nuclei consistently contain a single polycomplex. The behaviour, doubling and the fine structure of the spindle plaque provide additional markers for the different stages of meiosis.", "contents": "The synaptonemal complex and the spindle plaque during meiosis in yeast. Meiosis in Saccharomyces cerevisiae proceeds principally in the same manner as in other Ascomycetes. Leptotene is characterized by unpaired lateral components and pachytene by the presence of extensive synaptonemal complexes. The synaptonemal complex has the same dimensions and is similar in structure to those described for other organisms. Chromosome counts can now be made by reconstructing the synaptonemal complexes. Diplotene nuclei consistently contain a single polycomplex. The behaviour, doubling and the fine structure of the spindle plaque provide additional markers for the different stages of meiosis."}
{"id": "PMID:1093825", "title": "Lampbrush-type chromosomes in the primary nucleus of the green alga Acetabularia mediterranea.", "content": "Structures with a lampbruch-chromosome-like morphology are described in the nucleoplasm of primary nuclei of the green alga, Acetabularia mediterranea, by light and electron microscopy in sections of cells fixed in situ and in spread preparations of isolated nuclear components. These chromosomes reveal typical loops (up to 20 micronm long), chromomere-like nodules (1-2 micronm in diameter), and 2-4 micronm large axial globules. Associations of some of these chromosomes with nucleolar structures and with the nuclear envelope are also recognized. The light microscopically identified loops are correlated with distinct fibrillogranular structures observed in the thin sections and with the very long matrix units seen in the spread preparations. The similarity of these structures to the lampbrush chromosomes of various animal cell types, all exclusively stages of meiotic prophase, is discussed as well as the possible relation of the appearance of lampbrush chromosomes to a defined phase of the vegatative growth of this alga.", "contents": "Lampbrush-type chromosomes in the primary nucleus of the green alga Acetabularia mediterranea. Structures with a lampbruch-chromosome-like morphology are described in the nucleoplasm of primary nuclei of the green alga, Acetabularia mediterranea, by light and electron microscopy in sections of cells fixed in situ and in spread preparations of isolated nuclear components. These chromosomes reveal typical loops (up to 20 micronm long), chromomere-like nodules (1-2 micronm in diameter), and 2-4 micronm large axial globules. Associations of some of these chromosomes with nucleolar structures and with the nuclear envelope are also recognized. The light microscopically identified loops are correlated with distinct fibrillogranular structures observed in the thin sections and with the very long matrix units seen in the spread preparations. The similarity of these structures to the lampbrush chromosomes of various animal cell types, all exclusively stages of meiotic prophase, is discussed as well as the possible relation of the appearance of lampbrush chromosomes to a defined phase of the vegatative growth of this alga."}
{"id": "PMID:1093826", "title": "Investigation of cellular interaction and deployment in the early mammalian embryo using interspecific chimaeras between the rat and mouse.", "content": "Mammalian chimaeras can be produced experimentally by aggregating early embryos or by injecting cells into them.  They have been used to study several aspects of early development.  However, lack of a genetic marker enabling unequivocal identification of all cells of either genotype in situ has frustrated full exploitation of the experimental possibilities offered by these organisms.  Hence interspecific chimaeras have been produced between rat and mouse embryos in which cells of the two species can be identified in sectioned embryos by immune fluorescence.  These chimaeric embryos have been used to study differentiation of the trophoblast and inner cell mass, and the deployment of cells during morphogenesis.  Preliminary results suggest that the two tissues are determined by the blastocyst stage, and that the trophoblast forms part of the extra-embryonic membranes originally presumed to be derived from the inner cell mass.  Also, rat inner cell mass cells can induce mitosis in mouse trophoblast.  Futhermore, the distribution of rat cells in implanted embryos suggests that the embryo may grow in a coherent clonal manner from a very early stage.  Very recently, chimaerism has been induced by transplanting single rat cells, which may allow a more critical analysis of morphogenesis and determination than was possible hitherto.  An obvious question raised by crossing the species barrier is the extent to which results may be applicable to normal development.  Adverse effects of immunological interaction between the mouse uterine foster-mother and fetal rat cells, and sorting out of cells according to species, are two of the problems that might complicate interpretation of these experiments.", "contents": "Investigation of cellular interaction and deployment in the early mammalian embryo using interspecific chimaeras between the rat and mouse. Mammalian chimaeras can be produced experimentally by aggregating early embryos or by injecting cells into them.  They have been used to study several aspects of early development.  However, lack of a genetic marker enabling unequivocal identification of all cells of either genotype in situ has frustrated full exploitation of the experimental possibilities offered by these organisms.  Hence interspecific chimaeras have been produced between rat and mouse embryos in which cells of the two species can be identified in sectioned embryos by immune fluorescence.  These chimaeric embryos have been used to study differentiation of the trophoblast and inner cell mass, and the deployment of cells during morphogenesis.  Preliminary results suggest that the two tissues are determined by the blastocyst stage, and that the trophoblast forms part of the extra-embryonic membranes originally presumed to be derived from the inner cell mass.  Also, rat inner cell mass cells can induce mitosis in mouse trophoblast.  Futhermore, the distribution of rat cells in implanted embryos suggests that the embryo may grow in a coherent clonal manner from a very early stage.  Very recently, chimaerism has been induced by transplanting single rat cells, which may allow a more critical analysis of morphogenesis and determination than was possible hitherto.  An obvious question raised by crossing the species barrier is the extent to which results may be applicable to normal development.  Adverse effects of immunological interaction between the mouse uterine foster-mother and fetal rat cells, and sorting out of cells according to species, are two of the problems that might complicate interpretation of these experiments."}
{"id": "PMID:1093831", "title": "[Autologous renal transplantation for renovascular hypertension (author's transl)].", "content": "Renovascular hypertension was diagnosed in a 20-year-old man as being due to bilateral renal-artery stenoses and infrarenal aortic coarctation. Reconstruction of the renal arterial supply by vein graft and aortorenal bypass failed to reduce the hypertension. But after bilateral autotransplantation of the kidneys into the iliac fossa and a dacron lay-on graft over the coarcted region the arterial blood pressure returned to normal. Pre-operative measurement of plasma-renin concentration in renal vein blood and the patient's age are essential factors in deciding on reconstructive surgery of a stenosed renal artery.", "contents": "[Autologous renal transplantation for renovascular hypertension (author's transl)]. Renovascular hypertension was diagnosed in a 20-year-old man as being due to bilateral renal-artery stenoses and infrarenal aortic coarctation. Reconstruction of the renal arterial supply by vein graft and aortorenal bypass failed to reduce the hypertension. But after bilateral autotransplantation of the kidneys into the iliac fossa and a dacron lay-on graft over the coarcted region the arterial blood pressure returned to normal. Pre-operative measurement of plasma-renin concentration in renal vein blood and the patient's age are essential factors in deciding on reconstructive surgery of a stenosed renal artery."}
{"id": "PMID:1093834", "title": "[Anterior pituitary regulation of testicular function in the elderly (author's transl)].", "content": "Pituitary gonadotrophin regulation was examined with the LH-RH test in 15 men (aged between 55 and 90 years), five of them aged between 71 and 90 years. Compared to normal mature males, the plasma testosterone in these subjects was reduced or was in the lower range of normal, while basal LH levels were increased in half the cases. However, basal FSH values were increased in three only. In most of these subjects with increased basal values of LH, the LH-RH stimulation caused a paradoxical temporary reduction in LH release which did not occur with FSH. This paradoxical response also did not occur when basal LH values were normal.", "contents": "[Anterior pituitary regulation of testicular function in the elderly (author's transl)]. Pituitary gonadotrophin regulation was examined with the LH-RH test in 15 men (aged between 55 and 90 years), five of them aged between 71 and 90 years. Compared to normal mature males, the plasma testosterone in these subjects was reduced or was in the lower range of normal, while basal LH levels were increased in half the cases. However, basal FSH values were increased in three only. In most of these subjects with increased basal values of LH, the LH-RH stimulation caused a paradoxical temporary reduction in LH release which did not occur with FSH. This paradoxical response also did not occur when basal LH values were normal."}
{"id": "PMID:1093835", "title": "[Improving bacteriological diagnosis by using culture medium during transport of specimens (author's transl)].", "content": "It is well known that long transport diminishes the chances of isolating causative micro- organisms, especially from swabs. In a trial, in which beta-haemolytic streptococci were kept for 24-hours at room temperature, culturing failed even if more than one million bacteria had been placed on the swab. Culture medium consisting of agar with 0.5% human albumin made it possible to isolate beta-haemolytic streptococci even after transport and with a count of less than a hundred micro-organisms per swab. The use of selective nutrient media may be necessary because of the sometimes marked multiplication during transport when there are mixed organisms. The nutrient medium was used during six small epidemics of scarlet fever in Freiburg and proved superior to the usual dry swab. In parallel studies of patients and contacts the causative beta-haemolytic streptococcus was isolated three times as often.", "contents": "[Improving bacteriological diagnosis by using culture medium during transport of specimens (author's transl)]. It is well known that long transport diminishes the chances of isolating causative micro- organisms, especially from swabs. In a trial, in which beta-haemolytic streptococci were kept for 24-hours at room temperature, culturing failed even if more than one million bacteria had been placed on the swab. Culture medium consisting of agar with 0.5% human albumin made it possible to isolate beta-haemolytic streptococci even after transport and with a count of less than a hundred micro-organisms per swab. The use of selective nutrient media may be necessary because of the sometimes marked multiplication during transport when there are mixed organisms. The nutrient medium was used during six small epidemics of scarlet fever in Freiburg and proved superior to the usual dry swab. In parallel studies of patients and contacts the causative beta-haemolytic streptococcus was isolated three times as often."}
{"id": "PMID:1093838", "title": "[Studies on heat generation by drills of various abrasion grade].", "content": "By means of our own measuring method, drilling tests were performed at a test block made of silicate cement using rose-head and fissure burs as well as diamond-stones with different feed.  The temperature increases only within a limited range of wear and tear, it does not increase rectilinearly but stepwise, mainly influenced by two heat values, the shearing and the friction heat.  Having reached the temperature maximum, further drilling or further wear and tear only prolong drilling time.  The danger of damaging the pulp is primarily dependent on the feed and less on wear and tear, as was shown by the tests with the rose head burs.  Only when the feed is rising, the worn-out driller further increases a rise in temperature.", "contents": "[Studies on heat generation by drills of various abrasion grade]. By means of our own measuring method, drilling tests were performed at a test block made of silicate cement using rose-head and fissure burs as well as diamond-stones with different feed.  The temperature increases only within a limited range of wear and tear, it does not increase rectilinearly but stepwise, mainly influenced by two heat values, the shearing and the friction heat.  Having reached the temperature maximum, further drilling or further wear and tear only prolong drilling time.  The danger of damaging the pulp is primarily dependent on the feed and less on wear and tear, as was shown by the tests with the rose head burs.  Only when the feed is rising, the worn-out driller further increases a rise in temperature."}
{"id": "PMID:1093839", "title": "[Prosthetics and prevention of periodontal diseases].", "content": "This paper gives a survey of the present knowledge regarding prosthetical treatment under the aspects of preventing periodontal disease.  This survey is based on the German publications mainly of the past ten years.  In the beginning, the prevention of periodontal disease is weighed against the requirements of caries prophylaxis.  Then the requirements of periodontal hygiene are described that are inevitable for the beginning of treatment as well as for the dentures themselves.  The marginal periodontium that is in contact with the margin of the crown is generally regarded as an area of minor resistance, therefore particular attention should be given to this point of contact.  Construction of removable, partial dentures for partially edentulous jaws is also discussed in detail.  Final conclusions show that our knowledge in this specific field has been broadened.", "contents": "[Prosthetics and prevention of periodontal diseases]. This paper gives a survey of the present knowledge regarding prosthetical treatment under the aspects of preventing periodontal disease.  This survey is based on the German publications mainly of the past ten years.  In the beginning, the prevention of periodontal disease is weighed against the requirements of caries prophylaxis.  Then the requirements of periodontal hygiene are described that are inevitable for the beginning of treatment as well as for the dentures themselves.  The marginal periodontium that is in contact with the margin of the crown is generally regarded as an area of minor resistance, therefore particular attention should be given to this point of contact.  Construction of removable, partial dentures for partially edentulous jaws is also discussed in detail.  Final conclusions show that our knowledge in this specific field has been broadened."}
{"id": "PMID:1093840", "title": "[Economic value in the therapy of edentulous masticatory system].", "content": "In the present paper it is tried to analyze the economic value of prosthetical treatment of edentulous jaws which has to be performed from the physiological and gnathological viewpoints.  After defining the therapeutical objective and after  standardizing case histories, and medical examinations as well as therapy, well-known individual methodological measures are described which are indispensable for achieving the therapeutical aim.  Analysis of therapy, definition of therapeutical objective and standardization of case histories, medical examination, therapy, and methodological individual measures can be used for--rationalizing working procedures in the dental office, --evaluating new methods, --evaluating full dentures, --further elaborating ill-defined methodological steps, --investigating ill-defined therapeutical measures.", "contents": "[Economic value in the therapy of edentulous masticatory system]. In the present paper it is tried to analyze the economic value of prosthetical treatment of edentulous jaws which has to be performed from the physiological and gnathological viewpoints.  After defining the therapeutical objective and after  standardizing case histories, and medical examinations as well as therapy, well-known individual methodological measures are described which are indispensable for achieving the therapeutical aim.  Analysis of therapy, definition of therapeutical objective and standardization of case histories, medical examination, therapy, and methodological individual measures can be used for--rationalizing working procedures in the dental office, --evaluating new methods, --evaluating full dentures, --further elaborating ill-defined methodological steps, --investigating ill-defined therapeutical measures."}
{"id": "PMID:1093841", "title": "[Marker-evidence for documentation and evaluation of findings and treatment in fractures of the facial skull].", "content": "A special form is presented by means of which clinical findings and therapy of fractures of the visceral cranium are documented in such a way that the data can be fed into a computer and be evaluated.  Like similar forms for cheilognathopalatoschisis and tumors of the jaw and face area the present form is meant for a special field or specific problems and is not to replace the traditional case history.", "contents": "[Marker-evidence for documentation and evaluation of findings and treatment in fractures of the facial skull]. A special form is presented by means of which clinical findings and therapy of fractures of the visceral cranium are documented in such a way that the data can be fed into a computer and be evaluated.  Like similar forms for cheilognathopalatoschisis and tumors of the jaw and face area the present form is meant for a special field or specific problems and is not to replace the traditional case history."}
{"id": "PMID:1093843", "title": "[A new substituted benzamide with neuroleptic effects: lin 14 18 or sultopride].", "content": "After the research about Sulpiride, a new substituted benzamide has been studied. Although it was characterized by modest pharmacological effects in the neuroleptic identification tests, LIN 1418 turned out to have very interesting therapeutic properties. A study conducted with 50 cases, 30 of which were submitted a systematic pluridisciplinary control, leads to provisory conclusions as follows: 1) its psychotropic effects are powerful and constant; 2) its prevailing action is antipsychotic and desinhibiting altogether; 3) while LIN 1418 appears as successful with all psychoses, whether acute or chronic, up to now, the quality of its results in many chronic psychoses seems to be particularly remarkable; 4) its side-effects are those of most neuroleptics: its extrapyramidal symptoms being all the more obvious as the patient's \"passivity\" is less important.", "contents": "[A new substituted benzamide with neuroleptic effects: lin 14 18 or sultopride]. After the research about Sulpiride, a new substituted benzamide has been studied. Although it was characterized by modest pharmacological effects in the neuroleptic identification tests, LIN 1418 turned out to have very interesting therapeutic properties. A study conducted with 50 cases, 30 of which were submitted a systematic pluridisciplinary control, leads to provisory conclusions as follows: 1) its psychotropic effects are powerful and constant; 2) its prevailing action is antipsychotic and desinhibiting altogether; 3) while LIN 1418 appears as successful with all psychoses, whether acute or chronic, up to now, the quality of its results in many chronic psychoses seems to be particularly remarkable; 4) its side-effects are those of most neuroleptics: its extrapyramidal symptoms being all the more obvious as the patient's \"passivity\" is less important."}
{"id": "PMID:1093844", "title": "[Situation of sultopride among present-day neuroleptics].", "content": "The authors report preliminary clinical investigations about sultopride, a new substituted benzamid, related to sulpiride. The drug was administered to thirty-nine hospitalized psychotic patients. A very powerful and constant efficacy of sultopride was observed in 11 manic and hypomanic typical syndroms: excitation was controlled between the first and third day, with oral doses of 1,200-1,800 mg. But thymical inversion was observed in 8 cases (3 light anxious states and 5 typical melancholic syndroms). Besides, interesting improvements were obtained in atypical excitation disorders, chronic hallucinatory delusions, acute oniric and confusional states, schizophrenia and chronic alcoholism. Side-effects were frequent: extrapyramidal syndroms, often requiring antiparkinsonian correctors, somnolence, asthenia, and above all (in 30 percent of cases) psychical side-effects, consisting in depressive and anxious modifications of mood, even apart from manic-melancholic psychosis. This psychotropic depressive effect appears as very interesting theoretically, and justifies further enquiry.", "contents": "[Situation of sultopride among present-day neuroleptics]. The authors report preliminary clinical investigations about sultopride, a new substituted benzamid, related to sulpiride. The drug was administered to thirty-nine hospitalized psychotic patients. A very powerful and constant efficacy of sultopride was observed in 11 manic and hypomanic typical syndroms: excitation was controlled between the first and third day, with oral doses of 1,200-1,800 mg. But thymical inversion was observed in 8 cases (3 light anxious states and 5 typical melancholic syndroms). Besides, interesting improvements were obtained in atypical excitation disorders, chronic hallucinatory delusions, acute oniric and confusional states, schizophrenia and chronic alcoholism. Side-effects were frequent: extrapyramidal syndroms, often requiring antiparkinsonian correctors, somnolence, asthenia, and above all (in 30 percent of cases) psychical side-effects, consisting in depressive and anxious modifications of mood, even apart from manic-melancholic psychosis. This psychotropic depressive effect appears as very interesting theoretically, and justifies further enquiry."}
{"id": "PMID:1093845", "title": "[Comparison of clinical effects of delta-9-tetrahydrocannabinol with the classic effects of hashish].", "content": "Moreau de Tours's classical studies about haschisch had pointed out to a rich symptomatology: visual and auditive hallucinations preceded by the \"primordial effect\": \"the dissociation of ideas\". This delirious state was assimilated to dream. Modern studies, conducted with Delta-9-THC, in healthy voluntaries, again suggest the comparison or even the identity of the modifications caused by cannabis with sleep and dream. 10 mg of Delta-9-THC was absorbed in sesame oil by the voluntaries, and all of them presented disturbances of their vigilance, changes of their mood, now with euphoris and now with dysphoria, and slight psycho-sensorial alterations. The voluntaries EEG showed important and specific individual reactivity. Three subjects presented an arousal tendency, three other a greater towards sleep. Slight morphological changes have appeared, such as desynchronised periods, rapid shifts between I A, I B, II stages of sleep or an hypovariable I B stage of sedation with period of occular movements resembling to REM-stage.", "contents": "[Comparison of clinical effects of delta-9-tetrahydrocannabinol with the classic effects of hashish]. Moreau de Tours's classical studies about haschisch had pointed out to a rich symptomatology: visual and auditive hallucinations preceded by the \"primordial effect\": \"the dissociation of ideas\". This delirious state was assimilated to dream. Modern studies, conducted with Delta-9-THC, in healthy voluntaries, again suggest the comparison or even the identity of the modifications caused by cannabis with sleep and dream. 10 mg of Delta-9-THC was absorbed in sesame oil by the voluntaries, and all of them presented disturbances of their vigilance, changes of their mood, now with euphoris and now with dysphoria, and slight psycho-sensorial alterations. The voluntaries EEG showed important and specific individual reactivity. Three subjects presented an arousal tendency, three other a greater towards sleep. Slight morphological changes have appeared, such as desynchronised periods, rapid shifts between I A, I B, II stages of sleep or an hypovariable I B stage of sedation with period of occular movements resembling to REM-stage."}
{"id": "PMID:1093846", "title": "[Sulpiride and drug addiction].", "content": "The authors carried on their study as presented in Tunis Congress of Psychiatry and Neurology. The results of the administration of Sulpiride with drug-addicts are still difficult to evaluate, because none of the patients are hospitalized and there is no possibility to know the precise doses absorbed by the patients. An improvement is however obvious with patients who spontaneously come to ask for more. They generally present polytoxicomania and use haschisch, opium-derivatives, amphetamines and usually L.S.D. Two recent observations summarize the action of Sulpiride: 1. --A fit of acute depersonnalisation in a young lady was suppressed within eight days with the administration of 1 g p.o. of Sulpiride a day. Recovery without relapse. 2. --An old drug-addict would shoot himself i.v. with at least 1 g of Sulpiride whenever he wanted to stop his intoxication periods. He did not feel any \"flash\" but only could thus obtain a \"soft\" withdrawal without need syndrome. Neither dependence, nor serious side-effects were observed.", "contents": "[Sulpiride and drug addiction]. The authors carried on their study as presented in Tunis Congress of Psychiatry and Neurology. The results of the administration of Sulpiride with drug-addicts are still difficult to evaluate, because none of the patients are hospitalized and there is no possibility to know the precise doses absorbed by the patients. An improvement is however obvious with patients who spontaneously come to ask for more. They generally present polytoxicomania and use haschisch, opium-derivatives, amphetamines and usually L.S.D. Two recent observations summarize the action of Sulpiride: 1. --A fit of acute depersonnalisation in a young lady was suppressed within eight days with the administration of 1 g p.o. of Sulpiride a day. Recovery without relapse. 2. --An old drug-addict would shoot himself i.v. with at least 1 g of Sulpiride whenever he wanted to stop his intoxication periods. He did not feel any \"flash\" but only could thus obtain a \"soft\" withdrawal without need syndrome. Neither dependence, nor serious side-effects were observed."}
{"id": "PMID:1093847", "title": "[Clinical study of a new agent correcting the syndrome of neuroleptic impregnation in acute psychotic states].", "content": "The correcting action of tropatepine hydrochloride upon the extrapyramidal effects induced by neuroleptics has been studied in 32 acute psychotic states. A satisfying result has been obtained with daily doses of 10 to 20 mg in 30 cases (excellent results: 20, good results: 10 patients). The action upon dyskinetic crises is particularly good and quickly obtained, avoiding a recourse to an i.m. corrector in 7 cases out of 8. The akineto-hypertonic syndrome has been efficiently corrected in 18 cases out of 23. The hyperkinetic manifestations (akathisia, tasikinesia) reacted favourably in 8 cases out of 11, dysarthria in 7 cases out of 11. The lessening of tremor was less satisfying (8 cases out of 15); same thing for the dryness of the mouth (7 cases out of 16) and disorder of visual accomodation (6 cases out of 12). The clinical tolerance was excellent. With the used doses, we found neither inconvenient atropinic effect, nor disorder of the vigilance, nor pharmacodependence. The one daily administration of a 10 mg tablet turned out to be sufficient in 19 cases. This possibility of only one administration a day appeared to us as a great advantage.", "contents": "[Clinical study of a new agent correcting the syndrome of neuroleptic impregnation in acute psychotic states]. The correcting action of tropatepine hydrochloride upon the extrapyramidal effects induced by neuroleptics has been studied in 32 acute psychotic states. A satisfying result has been obtained with daily doses of 10 to 20 mg in 30 cases (excellent results: 20, good results: 10 patients). The action upon dyskinetic crises is particularly good and quickly obtained, avoiding a recourse to an i.m. corrector in 7 cases out of 8. The akineto-hypertonic syndrome has been efficiently corrected in 18 cases out of 23. The hyperkinetic manifestations (akathisia, tasikinesia) reacted favourably in 8 cases out of 11, dysarthria in 7 cases out of 11. The lessening of tremor was less satisfying (8 cases out of 15); same thing for the dryness of the mouth (7 cases out of 16) and disorder of visual accomodation (6 cases out of 12). The clinical tolerance was excellent. With the used doses, we found neither inconvenient atropinic effect, nor disorder of the vigilance, nor pharmacodependence. The one daily administration of a 10 mg tablet turned out to be sufficient in 19 cases. This possibility of only one administration a day appeared to us as a great advantage."}
{"id": "PMID:1093848", "title": "[A French experience with methadone].", "content": "Methadone chlorhydrate has been administred to opiate addicts either for a short period with a decreasing dosage as a withdrawal cure, or for longterm treatments as a basis of a maintenance program. The analysis of precise clinical criteria regarding the socio-professional states, the family-life, the general health and the psychopathological diagnosis, allows an evaluation of the success of the cure and of its elective indications. The study of the personnality of the drug-addicts shows the importance of this factor in the potential success of a methadone cure. Urinary biological controls are regularly performed. This limitations and promises of this treatment discussed form a basis of a year's experience.", "contents": "[A French experience with methadone]. Methadone chlorhydrate has been administred to opiate addicts either for a short period with a decreasing dosage as a withdrawal cure, or for longterm treatments as a basis of a maintenance program. The analysis of precise clinical criteria regarding the socio-professional states, the family-life, the general health and the psychopathological diagnosis, allows an evaluation of the success of the cure and of its elective indications. The study of the personnality of the drug-addicts shows the importance of this factor in the potential success of a methadone cure. Urinary biological controls are regularly performed. This limitations and promises of this treatment discussed form a basis of a year's experience."}
{"id": "PMID:1093849", "title": "Beta-N-acetylhexosaminidase active on dermatan sulfate.", "content": "The dodecasaccharide obtained by treating dermatan sulfate with testicular hyaluronidase, chondroitinase AC, and beta-glucuronidase was incubated with diluted, normal human serum at pH 4.5 or 7.0 followed by chondro-4-sulfatase at pH 7.0. Analyses of the reaction products indicate release of hexosamine but not further degradation of the substrate. It is concluded that normal human serum possesses an exo-beta-N-acetylhexosaminidase active on dermatan sulfate.", "contents": "Beta-N-acetylhexosaminidase active on dermatan sulfate. The dodecasaccharide obtained by treating dermatan sulfate with testicular hyaluronidase, chondroitinase AC, and beta-glucuronidase was incubated with diluted, normal human serum at pH 4.5 or 7.0 followed by chondro-4-sulfatase at pH 7.0. Analyses of the reaction products indicate release of hexosamine but not further degradation of the substrate. It is concluded that normal human serum possesses an exo-beta-N-acetylhexosaminidase active on dermatan sulfate."}
{"id": "PMID:1093854", "title": "Vagal role and pacemaker indication in hypersensitive carotid sinus reflex.", "content": "The effect of carotid sinus pressure (CSP) on sinus rate was studied in 24 patients with sinoatrial pauses below 3 sec (control) and 23 patients with sinoatrial pauses above 3 sec (hypersensitive carotid sinus reflex equal to (HCSR). For registration of P waves, intraatrial electrograms were used. CSP was applied several times before and after atropine. Patients with HCSR showed inconstant response to CSP. The maximal result was considered diagnostic. No difference in the lengths of sinoatrial pauses was found between patients without dizziness or syncopes in the presence of HCSR and patients with dizziness that proved clinically to be based on the existence of HCSR. It was concluded that CSP-induced asystole can serve neither as a differential diagnostic criterion nor as an indication for pacemaker application. Sick sinus syndrome, and thereby dysfunction of the sinus node, were excluded by rapid atrial stimulation. Patients with HCSR showed overall higher age, slower resting heart rate before and after atropine and less atropine response during CSP compared to control. Hypersensitivity of the carotid sinus reflex was interpreted as a part of the physiological augmentation of vagal tone in elderly people.", "contents": "Vagal role and pacemaker indication in hypersensitive carotid sinus reflex. The effect of carotid sinus pressure (CSP) on sinus rate was studied in 24 patients with sinoatrial pauses below 3 sec (control) and 23 patients with sinoatrial pauses above 3 sec (hypersensitive carotid sinus reflex equal to (HCSR). For registration of P waves, intraatrial electrograms were used. CSP was applied several times before and after atropine. Patients with HCSR showed inconstant response to CSP. The maximal result was considered diagnostic. No difference in the lengths of sinoatrial pauses was found between patients without dizziness or syncopes in the presence of HCSR and patients with dizziness that proved clinically to be based on the existence of HCSR. It was concluded that CSP-induced asystole can serve neither as a differential diagnostic criterion nor as an indication for pacemaker application. Sick sinus syndrome, and thereby dysfunction of the sinus node, were excluded by rapid atrial stimulation. Patients with HCSR showed overall higher age, slower resting heart rate before and after atropine and less atropine response during CSP compared to control. Hypersensitivity of the carotid sinus reflex was interpreted as a part of the physiological augmentation of vagal tone in elderly people."}
{"id": "PMID:1093883", "title": "Insulin biosynthesis and secretion.", "content": "Studies are presented which support the concept that a cell membrane localized glucoreceptor system is involved in the insulin secretory response to glucose and that the specific stimulation of insulin synthesis by glucose reflects effects at both the transcriptional and posttranscriptional level. After 48 hours of fasting the insulin secretory response to glucose is markedly reduced. This reduction is overcome by 24 hours of refeeding carbohydrate, but notprotein or fat, and is blocked by refeeding in the presence of an inhibitor of RNA synthesis. Although these studies clearly demonstrate an inducible glucoreceptor system, they do not permit conclusions regarding either its composition or location in the beta-cell. Phloridzin, a glycoside with a high affinity for glucose-carrier systems in plama membranes, stimulated basal insulin secretion sixfold. A large number of plant lectins were tested for ability to stimulate insulin release from isolated islets, and only mushroom lectin did so. The lectin concentration producing half-maximal hormone releaseis 3 x 10-7, a value in good agreement with the dissociation constant forlectin binding. Glucose stimulated insulin sythesis in isolated rat islets was determined to be partially inhibited by actinomycin D. The posttranscriptional effect was determined to be increased initiation of total islet mRNAas well as proinsulin mRNA. To futher quantitate the effect of glucose on proinsulin mRNA, immunoprecipitation of proinsulin synthesizing polysomes was accomplished. It appeared that proinsulin is synthesized on a mRNA accommodating six to eight ribosomes, and the size of the proinsulin mRNA is 10-11 S on sucrose gradients. This unexpectedly large size of the proinsulin mRNA is discussed.", "contents": "Insulin biosynthesis and secretion. Studies are presented which support the concept that a cell membrane localized glucoreceptor system is involved in the insulin secretory response to glucose and that the specific stimulation of insulin synthesis by glucose reflects effects at both the transcriptional and posttranscriptional level. After 48 hours of fasting the insulin secretory response to glucose is markedly reduced. This reduction is overcome by 24 hours of refeeding carbohydrate, but notprotein or fat, and is blocked by refeeding in the presence of an inhibitor of RNA synthesis. Although these studies clearly demonstrate an inducible glucoreceptor system, they do not permit conclusions regarding either its composition or location in the beta-cell. Phloridzin, a glycoside with a high affinity for glucose-carrier systems in plama membranes, stimulated basal insulin secretion sixfold. A large number of plant lectins were tested for ability to stimulate insulin release from isolated islets, and only mushroom lectin did so. The lectin concentration producing half-maximal hormone releaseis 3 x 10-7, a value in good agreement with the dissociation constant forlectin binding. Glucose stimulated insulin sythesis in isolated rat islets was determined to be partially inhibited by actinomycin D. The posttranscriptional effect was determined to be increased initiation of total islet mRNAas well as proinsulin mRNA. To futher quantitate the effect of glucose on proinsulin mRNA, immunoprecipitation of proinsulin synthesizing polysomes was accomplished. It appeared that proinsulin is synthesized on a mRNA accommodating six to eight ribosomes, and the size of the proinsulin mRNA is 10-11 S on sucrose gradients. This unexpectedly large size of the proinsulin mRNA is discussed."}
{"id": "PMID:1093889", "title": "Mitochondrial ATP-Pi exchange complex and the site of uncoupling of oxidative phosphorylation.", "content": "Five enzyme complexes, which are concerned with electron transport and oxidative phosphorylation, have been isolated from beef heart mitochondria. Enzyme complexes I, II, III and IV are the electron transfer complexes discovered in 1961. Complex V is an energy-conserving complex. It catalyzes ATP-Pi exchange and ATP hydrolysis. The exchange reaction is sensitive to uncouplers, rutamycin, valinomycin plus K-+, dicyclorexylcarboditmide, arsenate, azide, and adenylyl imidodiphosphate. It is also specific for ATP; ITP, GTP and UTP are essentially ineffective. Studies with the photoaffinity labeling uncoupler, 2-azido-4-nitrophenol (NPA), have shown that the mitochondrial uncoupler-binding sites are located exclusively in complex V. Complexes I, III and IV, which carry the three coupling sites of the respiratory chain, had negligible capacity for the binding of NPA, whereas the uncoupler-binding capacity of complex V appeared to be increased two- to threefold as compared to mitochondria. Complexes I, II, III, IV and V are obtained from the same batch of mitochondria by a simple fractionation procedure, which employs cholate, deoxycholate, ammonium acetate and ammonium sulfate. Studies with NPA have shown that mitochondria contain per milligram protein about 0.6 nmole of uniformly reacting uncoupler binding site. All of the uncouplers tested appeared to interact competitively with this site. Photoaffinity labeling with tritiated NPA has shown that a major portion of NPA binds to a polypeptide of molecular weight between 26,000 and 30,000. Other studies on the mechanism of uncoupling have shown that picrate is a membrane-impermeable uncoupler. It cannot uncouple mitochondria. However, it is an effective uncoupler of ATP synthesis and ATP-induced transhydrogenation or reverse electron transfer when used in conjunction with sonicated submitochondrial particles, which have an inside-out orientation of the inner membrane with respect to the medium. In these particles, picrate binds to the same uncoupler-binding site as NPA and other uncouplers. However, unlike the membrane-permeable uncouplers, picrate is a poor protonophore. It has a very small effect on the proton permeability of phosphorylating submitochondrial vesicles, even at two to three times the concentration needed for complete uncoupling. The increase in the proton permeability of submitochondrial vesicles caused by such high concentrations of picrate (500 mum) can be achieved with approximately 5 mum 2,4-dinitrophenol. At this concentration, dinitrophenol results in only about 20% uncoupling.", "contents": "Mitochondrial ATP-Pi exchange complex and the site of uncoupling of oxidative phosphorylation. Five enzyme complexes, which are concerned with electron transport and oxidative phosphorylation, have been isolated from beef heart mitochondria. Enzyme complexes I, II, III and IV are the electron transfer complexes discovered in 1961. Complex V is an energy-conserving complex. It catalyzes ATP-Pi exchange and ATP hydrolysis. The exchange reaction is sensitive to uncouplers, rutamycin, valinomycin plus K-+, dicyclorexylcarboditmide, arsenate, azide, and adenylyl imidodiphosphate. It is also specific for ATP; ITP, GTP and UTP are essentially ineffective. Studies with the photoaffinity labeling uncoupler, 2-azido-4-nitrophenol (NPA), have shown that the mitochondrial uncoupler-binding sites are located exclusively in complex V. Complexes I, III and IV, which carry the three coupling sites of the respiratory chain, had negligible capacity for the binding of NPA, whereas the uncoupler-binding capacity of complex V appeared to be increased two- to threefold as compared to mitochondria. Complexes I, II, III, IV and V are obtained from the same batch of mitochondria by a simple fractionation procedure, which employs cholate, deoxycholate, ammonium acetate and ammonium sulfate. Studies with NPA have shown that mitochondria contain per milligram protein about 0.6 nmole of uniformly reacting uncoupler binding site. All of the uncouplers tested appeared to interact competitively with this site. Photoaffinity labeling with tritiated NPA has shown that a major portion of NPA binds to a polypeptide of molecular weight between 26,000 and 30,000. Other studies on the mechanism of uncoupling have shown that picrate is a membrane-impermeable uncoupler. It cannot uncouple mitochondria. However, it is an effective uncoupler of ATP synthesis and ATP-induced transhydrogenation or reverse electron transfer when used in conjunction with sonicated submitochondrial particles, which have an inside-out orientation of the inner membrane with respect to the medium. In these particles, picrate binds to the same uncoupler-binding site as NPA and other uncouplers. However, unlike the membrane-permeable uncouplers, picrate is a poor protonophore. It has a very small effect on the proton permeability of phosphorylating submitochondrial vesicles, even at two to three times the concentration needed for complete uncoupling. The increase in the proton permeability of submitochondrial vesicles caused by such high concentrations of picrate (500 mum) can be achieved with approximately 5 mum 2,4-dinitrophenol. At this concentration, dinitrophenol results in only about 20% uncoupling."}
{"id": "PMID:1093892", "title": "Macrophage-lymphocyte interaction and antigen recognition.", "content": "The functional and morphologic aspects of immunologically relevant macrophage-lymphocyte interaction have been characterized in inbred guinea pigs. Antigen-dependent initiation of in vitro DNA synthesis in lymphocytes taken from animals previously immunized with that antigen has been shown to be macrophage dependent. Expression of this in vitro correlate of in vivo established cellular immunity requires that antigen is initially bound to macrophages antecedent to any interaction with the specific lymphocyte. In view of the failure of supernatants from macrophages briefly exposed to antigen to initiate DNA synthesis in immune lymphocytes and the critical density requirements for lymphocyte proliferation, it is reasonable to assume that a direct physical interaction occurs between the antigen-bearing macrophage and lymphocyte. Using an in vitro assay of lymphocyte binding to macrophage monolayers, we have characterized what appear to be sequential events in macrophage-lymphocyte interaction. The initial event is an antigen-independent interaction between macrophage and lymphocyte whose characteristics are rapid reversibility, species but not strain specificity, and dependence on macrophage metabolism. If macrophages bear antigen for which a given lymphocyte possesses a specific receptor, then a second nonreversible antigen-dependent stabilization of binding occurs. This latter step requires tht macrophage and lymphocyte be histocompatible. These observations parallel functional studies of the histocompatibility dependence of macrophage-associated antigen initiation of lymphocyte DNA synthesis in guinea pigs. A model sequence of the physical events correlating with functional macrophage-lymphocyte interaction is proposed.", "contents": "Macrophage-lymphocyte interaction and antigen recognition. The functional and morphologic aspects of immunologically relevant macrophage-lymphocyte interaction have been characterized in inbred guinea pigs. Antigen-dependent initiation of in vitro DNA synthesis in lymphocytes taken from animals previously immunized with that antigen has been shown to be macrophage dependent. Expression of this in vitro correlate of in vivo established cellular immunity requires that antigen is initially bound to macrophages antecedent to any interaction with the specific lymphocyte. In view of the failure of supernatants from macrophages briefly exposed to antigen to initiate DNA synthesis in immune lymphocytes and the critical density requirements for lymphocyte proliferation, it is reasonable to assume that a direct physical interaction occurs between the antigen-bearing macrophage and lymphocyte. Using an in vitro assay of lymphocyte binding to macrophage monolayers, we have characterized what appear to be sequential events in macrophage-lymphocyte interaction. The initial event is an antigen-independent interaction between macrophage and lymphocyte whose characteristics are rapid reversibility, species but not strain specificity, and dependence on macrophage metabolism. If macrophages bear antigen for which a given lymphocyte possesses a specific receptor, then a second nonreversible antigen-dependent stabilization of binding occurs. This latter step requires tht macrophage and lymphocyte be histocompatible. These observations parallel functional studies of the histocompatibility dependence of macrophage-associated antigen initiation of lymphocyte DNA synthesis in guinea pigs. A model sequence of the physical events correlating with functional macrophage-lymphocyte interaction is proposed."}
{"id": "PMID:1093893", "title": "Luteinizing hormone-releasing hormone in the treatment of anovultory infertility.", "content": "Fifteen infertile, anovulatory women, ages 20 to 37, were treated with synthetic luteinizing hormone-releasing hormone (LH-RH) to stimulate follicular maturation and/or ovulation. Thirteen of the patients had been treated unsuccessfully with other therapeutic regimens. To obtain follicular maturation, LH-RH was given intramuscularly in daily doses of 25 mu-g for 7 days and 50 mu-g for the next 7 days, or 100 mu-g for 3 days and 150 mu-g for the next 3 days. Both regimens were begun on the 4th day of a spontaneous or induced cycle. Follicular maturation (evaluated by the cervical mucus scale) occurred in three of seven cycles treated withe the first regimen and three of five treated with the second regimen. To induce ovulation, LH-RH was given in intravenous infusion (50 to 500 mu-g), intramuscularly (100 mu-g), or in a combination of both methods. The drug was administered after follicular maturation with LH-RH, clomiphene citrate, or human menopausal gonadotropin had been achieved; it was also given during four cycles in which spontaneous follicular maturation had occurred. Ovulation occurred in 10 of 25 treated cycles. Five pregnancies resulted, three in the first post-treatment cycle.", "contents": "Luteinizing hormone-releasing hormone in the treatment of anovultory infertility. Fifteen infertile, anovulatory women, ages 20 to 37, were treated with synthetic luteinizing hormone-releasing hormone (LH-RH) to stimulate follicular maturation and/or ovulation. Thirteen of the patients had been treated unsuccessfully with other therapeutic regimens. To obtain follicular maturation, LH-RH was given intramuscularly in daily doses of 25 mu-g for 7 days and 50 mu-g for the next 7 days, or 100 mu-g for 3 days and 150 mu-g for the next 3 days. Both regimens were begun on the 4th day of a spontaneous or induced cycle. Follicular maturation (evaluated by the cervical mucus scale) occurred in three of seven cycles treated withe the first regimen and three of five treated with the second regimen. To induce ovulation, LH-RH was given in intravenous infusion (50 to 500 mu-g), intramuscularly (100 mu-g), or in a combination of both methods. The drug was administered after follicular maturation with LH-RH, clomiphene citrate, or human menopausal gonadotropin had been achieved; it was also given during four cycles in which spontaneous follicular maturation had occurred. Ovulation occurred in 10 of 25 treated cycles. Five pregnancies resulted, three in the first post-treatment cycle."}
{"id": "PMID:1093894", "title": "Anti-luteinizing (LH)-releasing activity of several analogues of LH-releasing hormone.", "content": "The anti-LH-releasing activity of several analogues of LH-RH was tested by 2-hour infusion in ovariectomized, estrogen-progesterone-pretreated rats and in immature male rats. [Leu3]LH-RH, [Leu3, desGly10]-LH-RH ethylamide, [desHis2, Leu3,desGly10]-LH-RH ethylamide, [Gly2, Leu3,desGly10]-LH-RH ethylamide, [Leu1, desGly10]-LH-RH ethylamides, [desHis2,D-Ala6,desGly10]-LH-RH ethylamide, [desHis2,Leu3,D-Ala6, desGly10]-LH-RH ethylamide, and [D-pGlu1,desGly10]-LH-RH ethylamide, and and [D-pGlu1,desHis2,desGly10]-LH-RH ethylamide showed some anti-LH-releasing activity, but never completely inhibited the increase in serum LH in response to LH-RH. No significant differences were found among the analogues tested and [desHis2,desGly10]-LH-RH ethylamide, and inhibitory potency was not improved with those peptides containing D-alanine in position 6 of the chain. None of the analogues tested, including [desHis2,desGly10]-LH-RH ethylamide, was able to block the LH-RH-induced FSH release in these systems.", "contents": "Anti-luteinizing (LH)-releasing activity of several analogues of LH-releasing hormone. The anti-LH-releasing activity of several analogues of LH-RH was tested by 2-hour infusion in ovariectomized, estrogen-progesterone-pretreated rats and in immature male rats. [Leu3]LH-RH, [Leu3, desGly10]-LH-RH ethylamide, [desHis2, Leu3,desGly10]-LH-RH ethylamide, [Gly2, Leu3,desGly10]-LH-RH ethylamide, [Leu1, desGly10]-LH-RH ethylamides, [desHis2,D-Ala6,desGly10]-LH-RH ethylamide, [desHis2,Leu3,D-Ala6, desGly10]-LH-RH ethylamide, and [D-pGlu1,desGly10]-LH-RH ethylamide, and and [D-pGlu1,desHis2,desGly10]-LH-RH ethylamide showed some anti-LH-releasing activity, but never completely inhibited the increase in serum LH in response to LH-RH. No significant differences were found among the analogues tested and [desHis2,desGly10]-LH-RH ethylamide, and inhibitory potency was not improved with those peptides containing D-alanine in position 6 of the chain. None of the analogues tested, including [desHis2,desGly10]-LH-RH ethylamide, was able to block the LH-RH-induced FSH release in these systems."}
{"id": "PMID:1093895", "title": "Immunologic evidence for addition of oviductal components to the hamster zona pellucida.", "content": "An indirect immunofluorescent procedure was used to determine whether zonae of unfertilized and fertilized eggs would bind antibodies specific for hamster reproductive tract antigens. Appropriate controls indicated that the fluorescent staining was probably due to antigen-antibody interactions. Antigens were not detected in zonae of ovarian eggs. However, components in zonae of unfertilized eggs collected from the oviduct at metestrus, mid-diestrus, and late diestrus bound antibodies specific for oviductal and uterine antigens. The antigens appeared to be concentrated in a peripheral band within the zona. An outer area of the zona around the eggs obtained from the uterus at proestrus also bound the antibodies, although the fluorescent staining was relatively faint. Antigens were observed in the peripheral area of zonae of fertilized eggs collected from oviducts on days 1 and 2 postcoitum. However, the antibody-binding component of the zona was noticeably reduced in embryos taken from the uterus on day 3 of pregnancy. The results are consistent with the idea that the zonae of both unfertilized and fertilized hamster eggs are composed of layers and that the outer region contains specific antigens which are dereived from the oviduct. Furthermore, loss or alteration of these components in the zona of an embryo (but not of an unfertilized egg of the same postovulation age) may reflect some chemical or structural change which is a prerequisite for zona shedding. Anti-uterus sera (unabsorbed and absorbed) did not interfere with zona removal by trypsin, indicating that the antigens detected in the present study are different from the receptor sites which bind anti-ovary antibodies and certain plant agglutinins (as reported by other workers).", "contents": "Immunologic evidence for addition of oviductal components to the hamster zona pellucida. An indirect immunofluorescent procedure was used to determine whether zonae of unfertilized and fertilized eggs would bind antibodies specific for hamster reproductive tract antigens. Appropriate controls indicated that the fluorescent staining was probably due to antigen-antibody interactions. Antigens were not detected in zonae of ovarian eggs. However, components in zonae of unfertilized eggs collected from the oviduct at metestrus, mid-diestrus, and late diestrus bound antibodies specific for oviductal and uterine antigens. The antigens appeared to be concentrated in a peripheral band within the zona. An outer area of the zona around the eggs obtained from the uterus at proestrus also bound the antibodies, although the fluorescent staining was relatively faint. Antigens were observed in the peripheral area of zonae of fertilized eggs collected from oviducts on days 1 and 2 postcoitum. However, the antibody-binding component of the zona was noticeably reduced in embryos taken from the uterus on day 3 of pregnancy. The results are consistent with the idea that the zonae of both unfertilized and fertilized hamster eggs are composed of layers and that the outer region contains specific antigens which are dereived from the oviduct. Furthermore, loss or alteration of these components in the zona of an embryo (but not of an unfertilized egg of the same postovulation age) may reflect some chemical or structural change which is a prerequisite for zona shedding. Anti-uterus sera (unabsorbed and absorbed) did not interfere with zona removal by trypsin, indicating that the antigens detected in the present study are different from the receptor sites which bind anti-ovary antibodies and certain plant agglutinins (as reported by other workers)."}
{"id": "PMID:1093900", "title": "[Stationary exitation according to A.A. Ukhtomski\u012d and its role in the mechanism of completion of temporary connections].", "content": "When recording potentials form the cortical surface, an increase in the processes of local excitation was shown to occur at the moment of revealing the first signs of the conditioned reflex: appearing of slow waves, increasing amplitude of evoked potentials, negative shift of the cortical steady potential.  When recording evoked potentials from deep cortical layers, successive stages of transition of the local excitation into a spread one which carries out the conditioning, were shown.  Stationary depolarization occurring in conditioning and revealed in gross forms as slow waves, increasing amplitude of evoked potentials, and negative shift of steady potential, at the cellular level is revealed as formation of neuron's polysensory acitvity and is usually followed by increase in the action potential (AP) duration in neurons of the cortical ends of analysers participating in conditioning.  Etimizol is known to improve memory processes in man and in animals (2).  When acting on the mollusk neurons, etimizol increases AP duration by 144 plus or minus 64 percent.  The data obtained suggest that the observed in conditioning increase of AP durration of neurons in the cortical representation of conditioned and unconditioned stimuli is associated with alterations in ion exchange and with trace processes in neurons, which apparently is related to the fine mechanisms underlying the retention in memory.", "contents": "[Stationary exitation according to A.A. Ukhtomski\u012d and its role in the mechanism of completion of temporary connections]. When recording potentials form the cortical surface, an increase in the processes of local excitation was shown to occur at the moment of revealing the first signs of the conditioned reflex: appearing of slow waves, increasing amplitude of evoked potentials, negative shift of the cortical steady potential.  When recording evoked potentials from deep cortical layers, successive stages of transition of the local excitation into a spread one which carries out the conditioning, were shown.  Stationary depolarization occurring in conditioning and revealed in gross forms as slow waves, increasing amplitude of evoked potentials, and negative shift of steady potential, at the cellular level is revealed as formation of neuron's polysensory acitvity and is usually followed by increase in the action potential (AP) duration in neurons of the cortical ends of analysers participating in conditioning.  Etimizol is known to improve memory processes in man and in animals (2).  When acting on the mollusk neurons, etimizol increases AP duration by 144 plus or minus 64 percent.  The data obtained suggest that the observed in conditioning increase of AP durration of neurons in the cortical representation of conditioned and unconditioned stimuli is associated with alterations in ion exchange and with trace processes in neurons, which apparently is related to the fine mechanisms underlying the retention in memory."}
{"id": "PMID:1093901", "title": "[Role of A.A Ukhtomski\u012d in the development of the problems of work, working capacity and fatigue (peculiarities and characteristics of these states in various age periods)].", "content": "The data of research which has been carried out for years, are summarized.  They are presented in a succession conforming to the A.A. Ukhtomsky views on the gradual enlargement of amplitude of muscles contraction, on capacity for work, and on fatigue.  In the early postnatal period the phenomenon of gradual enlargement of amplitude of muscles contraction does not yet exist.  It only appears after realizaiton of standing posture, when skeletal muscles transit from the type of tonic activity to the phasic--tetanic type.  This phenomenon is due to that the first contractions induce hyperrelaxation which leads to the subsequent higher amplitude of contraction.  The steady state of work capacity of neuromuscular system without any changes of amplitude, in the conditions of undisturbed circulation in the organism as a whole, can be maintained in all the periods of age for a long time without any signs of fatigue.  This phenomenon takes place during the intervals between the contraction activity, the anabolic process having ample time to be completed.  Such a form of work capacity is called stability.  If the anabolic processes are unable to be completed during the intervals between the contractions, their amplitude gradually decreases reflecting the beginning of fatigue.  The contractile activity which is going on during the fatigue state, is called endurance.", "contents": "[Role of A.A Ukhtomski\u012d in the development of the problems of work, working capacity and fatigue (peculiarities and characteristics of these states in various age periods)]. The data of research which has been carried out for years, are summarized.  They are presented in a succession conforming to the A.A. Ukhtomsky views on the gradual enlargement of amplitude of muscles contraction, on capacity for work, and on fatigue.  In the early postnatal period the phenomenon of gradual enlargement of amplitude of muscles contraction does not yet exist.  It only appears after realizaiton of standing posture, when skeletal muscles transit from the type of tonic activity to the phasic--tetanic type.  This phenomenon is due to that the first contractions induce hyperrelaxation which leads to the subsequent higher amplitude of contraction.  The steady state of work capacity of neuromuscular system without any changes of amplitude, in the conditions of undisturbed circulation in the organism as a whole, can be maintained in all the periods of age for a long time without any signs of fatigue.  This phenomenon takes place during the intervals between the contraction activity, the anabolic process having ample time to be completed.  Such a form of work capacity is called stability.  If the anabolic processes are unable to be completed during the intervals between the contractions, their amplitude gradually decreases reflecting the beginning of fatigue.  The contractile activity which is going on during the fatigue state, is called endurance."}
{"id": "PMID:1093913", "title": "Insulin biosynthesis in isolated pancreatic islets of fetal and newborn rats.", "content": "The effect of glucose and glucose plus glucagon on the incorporation of H-3-L-leucine into proinsulin and insulin was examined in isolated islets of twenty-one-day old fetal and five- and ten-day old newborn rats. Maximal stimulation of (pro-) insulin biosynthesis was achieved with 100 mg. per cent of glucose in isolated islets of twenty-one-day old fetal rats. No additional effect was observed with 300 mg. per cent of glucose. On the other hand, in islets of five- and ten-day old newborn rats the incorporation of H-3-L-leucine into proinsulin and insulin was gradually augmented by glucose up to concentrations of 300 mg. per cent. Addition of glucagon to the various glucose concentrations only enhanced the synthesis of insulin in ten-day old newborn islets, whereas it had no effect on the islets of the younger age groups. The results show a different pattern of insulin biosyntheses in fetal and newborn islets, which may be related to the varied plasma glucose concentrations of the perinatal period.", "contents": "Insulin biosynthesis in isolated pancreatic islets of fetal and newborn rats. The effect of glucose and glucose plus glucagon on the incorporation of H-3-L-leucine into proinsulin and insulin was examined in isolated islets of twenty-one-day old fetal and five- and ten-day old newborn rats. Maximal stimulation of (pro-) insulin biosynthesis was achieved with 100 mg. per cent of glucose in isolated islets of twenty-one-day old fetal rats. No additional effect was observed with 300 mg. per cent of glucose. On the other hand, in islets of five- and ten-day old newborn rats the incorporation of H-3-L-leucine into proinsulin and insulin was gradually augmented by glucose up to concentrations of 300 mg. per cent. Addition of glucagon to the various glucose concentrations only enhanced the synthesis of insulin in ten-day old newborn islets, whereas it had no effect on the islets of the younger age groups. The results show a different pattern of insulin biosyntheses in fetal and newborn islets, which may be related to the varied plasma glucose concentrations of the perinatal period."}
{"id": "PMID:1093914", "title": "Pituitary responsiveness to luteinizing hormone-releasing hormone in insulin-dependent diabetes mellitus.", "content": "Impaired fertility and menstrual disturbances are common in diabetes mellitus. In order to study the effect of diabetes on gonadotropin secretion by the pituitary gland, twenty premenopausal diabetic females and seven diabetic males were investigated using luteinizing hormone-releasing hormone (LH-RH). Although the gonadotropes responded to LH-RH in the patients studied, a relative impairment was apparent when compared to normal matched control groups. In the female diabetic patients there was no difference in the gonadotropin responses obtained in those with oligomenorrhea or amenorrhea when compared to those with normal menses. A significant inverse relationship was found between the maximum rise in plasma luteinizing hormone and the fasting plasma glucose. These findings suggest an influence of glucose metabolism on pitiutary gonadotropin function. However, the reduced gonadotropin response is unlikely to be the sole cause of the abnormal gynecologic function.", "contents": "Pituitary responsiveness to luteinizing hormone-releasing hormone in insulin-dependent diabetes mellitus. Impaired fertility and menstrual disturbances are common in diabetes mellitus. In order to study the effect of diabetes on gonadotropin secretion by the pituitary gland, twenty premenopausal diabetic females and seven diabetic males were investigated using luteinizing hormone-releasing hormone (LH-RH). Although the gonadotropes responded to LH-RH in the patients studied, a relative impairment was apparent when compared to normal matched control groups. In the female diabetic patients there was no difference in the gonadotropin responses obtained in those with oligomenorrhea or amenorrhea when compared to those with normal menses. A significant inverse relationship was found between the maximum rise in plasma luteinizing hormone and the fasting plasma glucose. These findings suggest an influence of glucose metabolism on pitiutary gonadotropin function. However, the reduced gonadotropin response is unlikely to be the sole cause of the abnormal gynecologic function."}
{"id": "PMID:1093915", "title": "Sodium valproate in the treatment of intractable childhood epilepsy.", "content": "Sodium valproate has been used, in combination with a variety of standard anticonvulsant drugs, to treat 24 children with intractable epilepsy.  A reduction of fit frequency greater than 90 percent was obtained in six patients, and a reduction of over 50 per cent in another six, with an improvement in alertness and school performance in the majority of cases.  Grand mal, myoclonic and petit mal epilepsy gave the best response, while infantile spasms responded least well.  No serious side-effects were encountered.", "contents": "Sodium valproate in the treatment of intractable childhood epilepsy. Sodium valproate has been used, in combination with a variety of standard anticonvulsant drugs, to treat 24 children with intractable epilepsy.  A reduction of fit frequency greater than 90 percent was obtained in six patients, and a reduction of over 50 per cent in another six, with an improvement in alertness and school performance in the majority of cases.  Grand mal, myoclonic and petit mal epilepsy gave the best response, while infantile spasms responded least well.  No serious side-effects were encountered."}
{"id": "PMID:1093916", "title": "Correction of equinus in cerebral palsy by the Murphy procedure of tendo calcaneus advancement: a preliminary communication.", "content": "The biomechanics of the Murphy procedure for correction of equinus in cerebral palsy are presented.  A series of 79 surgical procedures on 48 patients, with a follow-up period of from one to four years, is reviewed.  Correction of equinus was succeswful in 89-9 per cent of the procedures.  Using the Murphy procedure the following advantages over other methods of correction are anticipated: (1) no loss on 'push-off' in gait; (2) no loss of correction (recurrence) during longitudinal growth of the child, necessitating repeated surgery; and (3) no need for continual night bracing during the growth period.", "contents": "Correction of equinus in cerebral palsy by the Murphy procedure of tendo calcaneus advancement: a preliminary communication. The biomechanics of the Murphy procedure for correction of equinus in cerebral palsy are presented.  A series of 79 surgical procedures on 48 patients, with a follow-up period of from one to four years, is reviewed.  Correction of equinus was succeswful in 89-9 per cent of the procedures.  Using the Murphy procedure the following advantages over other methods of correction are anticipated: (1) no loss on 'push-off' in gait; (2) no loss of correction (recurrence) during longitudinal growth of the child, necessitating repeated surgery; and (3) no need for continual night bracing during the growth period."}
{"id": "PMID:1093919", "title": "Factors affecting the concentration of combustible gases in the colon during colonoscopy.", "content": "The colonic concentrations of the combustible gases, H2 and CH4, were well below hazardous levels in 60 consecutive patients at the time of colonoscopy. Independent analysis of the effect of a low residue liquid diet, a 12-hr fast, and bowel cleansing on the pulmonary excretion of these gases suggest that the low colonic concentrations encountered were largely the result of our patient preparation procedure. Both ingestion of the liquid diet and fasting decreased the pulmonary excretion of H2 markedly but caused only a slight fall in CH4 excretion. Thus H2, but not CH4, production appears to depend on the delivery to the colonic bacteria of exogenous fermentable substrate. Bulk removal of bacteria from the colon resulted in about a 10-fold reduction in the excretion of both gases. The results of these studies do not support the need for routine CO2 insufflation prior to colonoscopic electrosurgical polypectomy.", "contents": "Factors affecting the concentration of combustible gases in the colon during colonoscopy. The colonic concentrations of the combustible gases, H2 and CH4, were well below hazardous levels in 60 consecutive patients at the time of colonoscopy. Independent analysis of the effect of a low residue liquid diet, a 12-hr fast, and bowel cleansing on the pulmonary excretion of these gases suggest that the low colonic concentrations encountered were largely the result of our patient preparation procedure. Both ingestion of the liquid diet and fasting decreased the pulmonary excretion of H2 markedly but caused only a slight fall in CH4 excretion. Thus H2, but not CH4, production appears to depend on the delivery to the colonic bacteria of exogenous fermentable substrate. Bulk removal of bacteria from the colon resulted in about a 10-fold reduction in the excretion of both gases. The results of these studies do not support the need for routine CO2 insufflation prior to colonoscopic electrosurgical polypectomy."}
{"id": "PMID:1093920", "title": "Depression of antral and serum gastrin concentration by food deprivation in the rat.", "content": "In rats fasted 4 days, immunoreactive gastrin concentrations decreased to one-third of the fed levels in antral tissue and to one-eight of the fed levels in serum. The number of antral cells that reacted with fluorescent antigastrin antiserum was also correspondingly decreased. After refeeding, serum gastrin returned to normal levels in 6 days, whereas antral gastrin concentration recovered after 9 days. Normal gastrin levels were maintained in rats fed a nutritious liquid diet over a 6-day period, whereas tissue and serum hormone concentrations decreased to low levels in rats placed on a high bulk non-nutritive diet over the same period. These results suggest that food in the gastrointestinal tract is necessary for the maintenance of normal serum and antral gastrin concentration in rats. The effect of food is most likely attributable to chemical constituents and not distention by bulk.", "contents": "Depression of antral and serum gastrin concentration by food deprivation in the rat. In rats fasted 4 days, immunoreactive gastrin concentrations decreased to one-third of the fed levels in antral tissue and to one-eight of the fed levels in serum. The number of antral cells that reacted with fluorescent antigastrin antiserum was also correspondingly decreased. After refeeding, serum gastrin returned to normal levels in 6 days, whereas antral gastrin concentration recovered after 9 days. Normal gastrin levels were maintained in rats fed a nutritious liquid diet over a 6-day period, whereas tissue and serum hormone concentrations decreased to low levels in rats placed on a high bulk non-nutritive diet over the same period. These results suggest that food in the gastrointestinal tract is necessary for the maintenance of normal serum and antral gastrin concentration in rats. The effect of food is most likely attributable to chemical constituents and not distention by bulk."}
{"id": "PMID:1093921", "title": "Significance of elevated liver alkaline phosphatase in serum.", "content": "The serum alkaline phosphatase was fractionated by polyacrylamide gel electrophoresis in 317 patients with elevated serum alkaline phosphatase activity. In 253 patients the source of the elevation was the isoenzyme of presumed liver origin, band L. In 87 of these patients, there was either no obvious liver disease or the alkaline phosphatase elevation was inappropriately high. In 19 of the 87, liver disease was further excluded by liver biopsy or by laparotomy. Because of this, biochemical studies were done to verify the hepatic origin of band L. Band L and alkaline phosphatase extracted from human liver migrated together on polyacrylamide gel electrophoresis before and after digestion with Vibrio cholerae neuraminidase. They had identical pH optima, sedimentation coefficients, Michaelis constants, and rates of inactivation at 55.5 degrees C. They had different rates of inactivation in 3 M urea. Over-all, the data indicate that band L is of liver origin, and that elevation of the hepatic alkaline phosphatase isoenzyme may be a nonspecific finding in certain patients.", "contents": "Significance of elevated liver alkaline phosphatase in serum. The serum alkaline phosphatase was fractionated by polyacrylamide gel electrophoresis in 317 patients with elevated serum alkaline phosphatase activity. In 253 patients the source of the elevation was the isoenzyme of presumed liver origin, band L. In 87 of these patients, there was either no obvious liver disease or the alkaline phosphatase elevation was inappropriately high. In 19 of the 87, liver disease was further excluded by liver biopsy or by laparotomy. Because of this, biochemical studies were done to verify the hepatic origin of band L. Band L and alkaline phosphatase extracted from human liver migrated together on polyacrylamide gel electrophoresis before and after digestion with Vibrio cholerae neuraminidase. They had identical pH optima, sedimentation coefficients, Michaelis constants, and rates of inactivation at 55.5 degrees C. They had different rates of inactivation in 3 M urea. Over-all, the data indicate that band L is of liver origin, and that elevation of the hepatic alkaline phosphatase isoenzyme may be a nonspecific finding in certain patients."}
{"id": "PMID:1093922", "title": "Evaluation of radiographic lucency or opaqueness of gallstones as a means of identifying cholesterol or pigment stones. Correlation of lucency or opaqueness with calcium and mineral.", "content": "A major criterion for the selection of patients with gallstones for treatment with chenodeoxycholic acid is the radiographic demonstration of lucent gallstones. In this study, we sought to evaluate the degree of selectivity of that criterion for distinguishing patients with cholesterol stones from those with pigment stones and to define the determinants of stone lucency or opaqueness. Of 92 lucent stones, 14% were pigment stones; and of 18 of opaque stones, 33% were cholesterol. Thus, the criterion of stone lucency allows inclusion of a significant number of subjects (14%) with lucent pigment stones, which may account for about one-half of the reported 33% incidence of treatment failures with chenodeoxycholic acid. Conversely, of patients with opaque stones, the one-third with cholesterol stones would be excluded from chenodeoxycholic acid treatment. Calcium is the major metal of both types of stones. However, opaque stones contain 6 times more calcium than lucent stones, which accounts for the difference in radiographic appearance.", "contents": "Evaluation of radiographic lucency or opaqueness of gallstones as a means of identifying cholesterol or pigment stones. Correlation of lucency or opaqueness with calcium and mineral. A major criterion for the selection of patients with gallstones for treatment with chenodeoxycholic acid is the radiographic demonstration of lucent gallstones. In this study, we sought to evaluate the degree of selectivity of that criterion for distinguishing patients with cholesterol stones from those with pigment stones and to define the determinants of stone lucency or opaqueness. Of 92 lucent stones, 14% were pigment stones; and of 18 of opaque stones, 33% were cholesterol. Thus, the criterion of stone lucency allows inclusion of a significant number of subjects (14%) with lucent pigment stones, which may account for about one-half of the reported 33% incidence of treatment failures with chenodeoxycholic acid. Conversely, of patients with opaque stones, the one-third with cholesterol stones would be excluded from chenodeoxycholic acid treatment. Calcium is the major metal of both types of stones. However, opaque stones contain 6 times more calcium than lucent stones, which accounts for the difference in radiographic appearance."}
{"id": "PMID:1093930", "title": "Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations.", "content": "We have isolated a new class of deletion mutants of phage lambda that extend from the prophage attachment site, att, into the gam and cIII genes. In this respect they are similar to certain of the lambda pbio transducing phage, but they differ in having a low burst size and in forming minute plaques. Lytically grown stocks of the deletions contain a variable proportion of phage that produce large plaques. These have been shown to carry an additional point mutation. Similar mutations, called chi, have been described by Lam et al. (1974), who showed that they result in a hot-spot for recombination produced by the host recombination system (Rec). We show that chi mutations can occurat several sites in the lambda genome and produce a Rec-dependent increase in the burst size of the one deletion tested.---In addition to reducing burst size, the one deletion tested reduces synthesis of DNA  and emdolysin but increases production of serum blocking protein. A chi mutation partially restores DNA synthesis and endolysin production and reduces serum blocking protein to normal levels. Our results are consistent with the hypothesis put forward by Lam et al., that chi enhances the frequency of Rec-promoted recombination, which provides the only pathway for production of maturable DNAin a red gam infection. The mechanism of the differential effect on protein production is, however, unclear.---Chi mutations are found to occur in DNA other than that of lambda. We show that, as has been suggested elsewhere (McMilin, Stahl and Stahy 1974), the lambda pbio transducing phages carry a chi mutation within the E. coli DNA substitution. A chi mutation also arose in a new substitution of unknown origin isolated in the course of this work.", "contents": "Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations. We have isolated a new class of deletion mutants of phage lambda that extend from the prophage attachment site, att, into the gam and cIII genes. In this respect they are similar to certain of the lambda pbio transducing phage, but they differ in having a low burst size and in forming minute plaques. Lytically grown stocks of the deletions contain a variable proportion of phage that produce large plaques. These have been shown to carry an additional point mutation. Similar mutations, called chi, have been described by Lam et al. (1974), who showed that they result in a hot-spot for recombination produced by the host recombination system (Rec). We show that chi mutations can occurat several sites in the lambda genome and produce a Rec-dependent increase in the burst size of the one deletion tested.---In addition to reducing burst size, the one deletion tested reduces synthesis of DNA  and emdolysin but increases production of serum blocking protein. A chi mutation partially restores DNA synthesis and endolysin production and reduces serum blocking protein to normal levels. Our results are consistent with the hypothesis put forward by Lam et al., that chi enhances the frequency of Rec-promoted recombination, which provides the only pathway for production of maturable DNAin a red gam infection. The mechanism of the differential effect on protein production is, however, unclear.---Chi mutations are found to occur in DNA other than that of lambda. We show that, as has been suggested elsewhere (McMilin, Stahl and Stahy 1974), the lambda pbio transducing phages carry a chi mutation within the E. coli DNA substitution. A chi mutation also arose in a new substitution of unknown origin isolated in the course of this work."}
{"id": "PMID:1093931", "title": "A new method for mutant selection in Saccharomyces cerevisiae.", "content": "A new method for the selection of auxotrophic, antibiotic- and temperature=sensitivemutants in Saccharomyces cerevisiae is reported. The technique is based upon the observation that certain fatty acid auxotrophs of yeast die when deprived of fatty acid only under conditions supporting growth. When macromolecular synthesis is blocked, the fattyacid-starved cells survive. By appropriate manipulation of a fatty acid-requiring strain enrichment as great as 75-fold was achieved for certain classes of auxotrophic mutants. An enrichment of approximately 100-fold is possible for some antibiotic-sensitive mutants. Selection for temperature-sensitive mutants, however, resulted in less than a 2-fold increase in the frequency of such mutants, probably because of the heterogeneity ofthis mutant category. It is likely that only that fraction of temperature-sensitivemutations which rapidly and reversibly blocks macromolecular synthesis is selected by this technique.", "contents": "A new method for mutant selection in Saccharomyces cerevisiae. A new method for the selection of auxotrophic, antibiotic- and temperature=sensitivemutants in Saccharomyces cerevisiae is reported. The technique is based upon the observation that certain fatty acid auxotrophs of yeast die when deprived of fatty acid only under conditions supporting growth. When macromolecular synthesis is blocked, the fattyacid-starved cells survive. By appropriate manipulation of a fatty acid-requiring strain enrichment as great as 75-fold was achieved for certain classes of auxotrophic mutants. An enrichment of approximately 100-fold is possible for some antibiotic-sensitive mutants. Selection for temperature-sensitive mutants, however, resulted in less than a 2-fold increase in the frequency of such mutants, probably because of the heterogeneity ofthis mutant category. It is likely that only that fraction of temperature-sensitivemutations which rapidly and reversibly blocks macromolecular synthesis is selected by this technique."}
{"id": "PMID:1093932", "title": "Recessive lethal amber suppressors in yeast.", "content": "Recessive lethal amber suppressor mutations have been isolated in a diploid strain of Saccharomyces cerevisiae. Diploids carrying these suppressors upon sporulation yield asci with only two live spores, both lacking the suppressor. At least two classes of recessive lethal suppressors exist. Aneuploid strains carrying one wild type and one suppressor locus have been isolated and used in mapping studies; one suppressor maps on chromosome III, the other does not.", "contents": "Recessive lethal amber suppressors in yeast. Recessive lethal amber suppressor mutations have been isolated in a diploid strain of Saccharomyces cerevisiae. Diploids carrying these suppressors upon sporulation yield asci with only two live spores, both lacking the suppressor. At least two classes of recessive lethal suppressors exist. Aneuploid strains carrying one wild type and one suppressor locus have been isolated and used in mapping studies; one suppressor maps on chromosome III, the other does not."}
{"id": "PMID:1093933", "title": "Evidence of preferential pairing of chromosomes at meiosis in aneuploid yeast.", "content": "Meiotic pairing in homothallic S. cerevisiae was studied by tetrad analysis, using strains that were trisomic or tetrasomic for chromosomes. I. The disomic segregants of these strains produce tetrasomic spore colonies that can be distinguished by their phenotype. Results indicated the existence of preferential pairing and nonrandom assortment of chromosomes at meisosis I. The frequency of crossing over is apparently normal in at least some regions when nonpreferred pairing occurs.", "contents": "Evidence of preferential pairing of chromosomes at meiosis in aneuploid yeast. Meiotic pairing in homothallic S. cerevisiae was studied by tetrad analysis, using strains that were trisomic or tetrasomic for chromosomes. I. The disomic segregants of these strains produce tetrasomic spore colonies that can be distinguished by their phenotype. Results indicated the existence of preferential pairing and nonrandom assortment of chromosomes at meisosis I. The frequency of crossing over is apparently normal in at least some regions when nonpreferred pairing occurs."}
{"id": "PMID:1093934", "title": "Requirement for cell dispersion prior to selection of induced azaguanine-resistant colonies of Chinese hamster cells.", "content": "With V79 Chinese hamster cell cultures treated with a mutagen, the maximum frequency of colonies resistant to 8-azaguanine (AZG) was attained when the cells were dispersed after a suitable expression time before adding the selection medium. V79-4 cells were exposed to 500 muM MMS, 7 muM AFAA, or 10 muM MNNG and allowed to multiply before being reseeded at 4 times 10-4 cells/60 mm dish and selected with 10 mu-g/ml AZG. Maximum frequencies of 4 times 10-5, 4 times 10-4, and 2.4 times 10-3 were obtained about 100, 130, and 200 hrs after exposure to MMS, AFAA, and MNNG, respectively. The maximum frequencies following MMS or MNNG treatments were about 10-fold greater than those obtained when induction and selection of AZG-resistant colonies were performed in the same culture dish. The reseeding of treated cells eliminated the possibility of metabolic cooperation within mosaic colonies of wild-type and mutant cells and achieved expression of the induced changes before intercolony crossfeeding reduced the frequency of resistant colonies. -  AZG-resistant colonies were selected in medium containing dialyzed fetal bovine serum, and the selection medium replacement were necessary for consistent achievement of background frequencies of resistant colonies near 10-6. Reconstruction experiments with AZG-resistant V79 lines showed that the efficiency of recovery of resistant cells in the selection medium was constant over a range of 0-20 colonies observed/dish. A mixed population of V79 and AZG-resistant cells was also correctly analyzed by the procedure used in mutagenesis studies.", "contents": "Requirement for cell dispersion prior to selection of induced azaguanine-resistant colonies of Chinese hamster cells. With V79 Chinese hamster cell cultures treated with a mutagen, the maximum frequency of colonies resistant to 8-azaguanine (AZG) was attained when the cells were dispersed after a suitable expression time before adding the selection medium. V79-4 cells were exposed to 500 muM MMS, 7 muM AFAA, or 10 muM MNNG and allowed to multiply before being reseeded at 4 times 10-4 cells/60 mm dish and selected with 10 mu-g/ml AZG. Maximum frequencies of 4 times 10-5, 4 times 10-4, and 2.4 times 10-3 were obtained about 100, 130, and 200 hrs after exposure to MMS, AFAA, and MNNG, respectively. The maximum frequencies following MMS or MNNG treatments were about 10-fold greater than those obtained when induction and selection of AZG-resistant colonies were performed in the same culture dish. The reseeding of treated cells eliminated the possibility of metabolic cooperation within mosaic colonies of wild-type and mutant cells and achieved expression of the induced changes before intercolony crossfeeding reduced the frequency of resistant colonies. -  AZG-resistant colonies were selected in medium containing dialyzed fetal bovine serum, and the selection medium replacement were necessary for consistent achievement of background frequencies of resistant colonies near 10-6. Reconstruction experiments with AZG-resistant V79 lines showed that the efficiency of recovery of resistant cells in the selection medium was constant over a range of 0-20 colonies observed/dish. A mixed population of V79 and AZG-resistant cells was also correctly analyzed by the procedure used in mutagenesis studies."}
{"id": "PMID:1093935", "title": "Inositol-requiring mutants of Saccharomyces cerevisiae.", "content": "Fifty-two inositol-requiring mutants of Saccharomyces cerevisiae were isolated following mutagenesis with ethyl methanesulfonate. Complementation and tetrad analysis revealed ten major complementation classes, representing ten independently segregating loci (designated ino1 through ino10) which recombined freely with their respective centromeres. Members of any given complementation class segregated as alleles of a single locus. Thirteen complementation subclasses were identified among thirty-six mutants which behaved as alleles of the ino1 locus. The complementation map for these mutants was circular. - Dramatic cell viability losses indicative of unbalanced growth were observed in liquid cultures of representative mutants under conditions of inositol starvation. Investigation of the timing, kinetics, and extent of cell death revealed that losses in cell viability in the range of 2-4 log orders could be prevented by the addition of inositol to the medium or by disruption of protein synthesis with cycloheximide. Mutants defective in nine of the ten loci identified in this study displayed these unusual characteristics. The results suggest an important physiological role for inositol that may be related to its cellular localization and function in membrane phospholipids. The possibility is discussed that inositol deficiency initiates the process of unbalanced growth leading to cell death through the loss of normal assembly, function, or integrity of biomembranes. - Part of this work has been reported in preliminary form (CULBERTSON and HENRY 1974).", "contents": "Inositol-requiring mutants of Saccharomyces cerevisiae. Fifty-two inositol-requiring mutants of Saccharomyces cerevisiae were isolated following mutagenesis with ethyl methanesulfonate. Complementation and tetrad analysis revealed ten major complementation classes, representing ten independently segregating loci (designated ino1 through ino10) which recombined freely with their respective centromeres. Members of any given complementation class segregated as alleles of a single locus. Thirteen complementation subclasses were identified among thirty-six mutants which behaved as alleles of the ino1 locus. The complementation map for these mutants was circular. - Dramatic cell viability losses indicative of unbalanced growth were observed in liquid cultures of representative mutants under conditions of inositol starvation. Investigation of the timing, kinetics, and extent of cell death revealed that losses in cell viability in the range of 2-4 log orders could be prevented by the addition of inositol to the medium or by disruption of protein synthesis with cycloheximide. Mutants defective in nine of the ten loci identified in this study displayed these unusual characteristics. The results suggest an important physiological role for inositol that may be related to its cellular localization and function in membrane phospholipids. The possibility is discussed that inositol deficiency initiates the process of unbalanced growth leading to cell death through the loss of normal assembly, function, or integrity of biomembranes. - Part of this work has been reported in preliminary form (CULBERTSON and HENRY 1974)."}
{"id": "PMID:1093936", "title": "Mating type and sporulation in yeast. I. Mutations which alter mating-type control over sporulation.", "content": "In Saccharomyces cerevisiae, meiosis and spore formation as well as mating are controlled by mating-type genes. Diploids heterozygous for mating type (aalpha) can sporulate but cannot mate; homozygous aa and alpha-alpha diploids can mate, but cannot sporulate. From an alpha-alpha diploid parental strain, we have isolated mutants which have gained the ability to sporulate. Those mutants which continue to mate as alpha-alpha cells have been designated CSP (control of sporulation). Upon sporulation, CSP mutants yield asci containing 4alpha spores. The mutant gene which allows alpha-alpha cells to sporulate is unlinked to the mating-type locus and also acts to permit sporulation in aa diploid cells. Segregation data from crosses between mutant alpha-alpha and wild-type aa diploids and vice versa indicate (for all but one mutant) that the mutation which allows constitutive sporulation (CSP) is dominant over the wild-type allele. Some of the CSP mutants are temperature-sensitive, sporulating at 32 degrees, but not at 23 degrees. In addition to CSP mutants, our mutagenesis and screening procedure led to the isolation of mutants which sporulate by virtue of a change in the mating-type locus itself, resulting in loss of ability to mate.", "contents": "Mating type and sporulation in yeast. I. Mutations which alter mating-type control over sporulation. In Saccharomyces cerevisiae, meiosis and spore formation as well as mating are controlled by mating-type genes. Diploids heterozygous for mating type (aalpha) can sporulate but cannot mate; homozygous aa and alpha-alpha diploids can mate, but cannot sporulate. From an alpha-alpha diploid parental strain, we have isolated mutants which have gained the ability to sporulate. Those mutants which continue to mate as alpha-alpha cells have been designated CSP (control of sporulation). Upon sporulation, CSP mutants yield asci containing 4alpha spores. The mutant gene which allows alpha-alpha cells to sporulate is unlinked to the mating-type locus and also acts to permit sporulation in aa diploid cells. Segregation data from crosses between mutant alpha-alpha and wild-type aa diploids and vice versa indicate (for all but one mutant) that the mutation which allows constitutive sporulation (CSP) is dominant over the wild-type allele. Some of the CSP mutants are temperature-sensitive, sporulating at 32 degrees, but not at 23 degrees. In addition to CSP mutants, our mutagenesis and screening procedure led to the isolation of mutants which sporulate by virtue of a change in the mating-type locus itself, resulting in loss of ability to mate."}
{"id": "PMID:1093937", "title": "Mating type and sporulation in yeast. II. Meiosis, recombination, and radiation sensitivity in an alpha-alpha diploid with altered sporulation control.", "content": "In wild-type S. cerevisiae, diploid cells must be heterozygous at the mating-type locus in order to sporulate. In the preceding paper, we described a number of mutants (CSP mutants), isolated from nonsporulating aa and alpha-alpha parent strains, in which sporulation appeared to be uncoupled from control by mating type. The characterization of one of these mutants (CSP1) is now extended to other processes controlled by mating type. This mutant is indistinguishable from alpha-alpha cells and unlike aalpha cells for mating factor production and response, zygote formation, intragenic mitotic recombination, and for X-ray sensitivity. The mutant apparently undergoes a full round of DNA synthesis in sporulation medium, but with delayed kinetics. Only 20% of the cells complete sporulation. Among spores in completed asci, the frequency of both intra- and intergenic recombination is the same as it is for spores produced by aalpha cells. However, experiments in which cells were shifted from sporulation medium back to minimal growth medium gave a frequency of meiotic recombination between ade2 or leu2 heteroalleles only 25% to 29% as high for CSP1 alpha-alpha diploid or CSP1 aa disomic cells as for aalpha diploid or disomic cells. Because the latter result, indicating recombination defectiveness, measured recombinant production in the entire cell population, whereas the result indicating normal recombination sampled only completed spores, we infer that all meiotic recombination events occuring in the population of CSP1 alpha-alpha cells are concentrated in those few cells which complete sporulation. This high degree of correlation between meiotic recombination and the completion of meiosis and sporulation suggests that recombination may be required for proper meiotic chromosome segregation in yeast just as it appears to be in maize and in Drosophila.", "contents": "Mating type and sporulation in yeast. II. Meiosis, recombination, and radiation sensitivity in an alpha-alpha diploid with altered sporulation control. In wild-type S. cerevisiae, diploid cells must be heterozygous at the mating-type locus in order to sporulate. In the preceding paper, we described a number of mutants (CSP mutants), isolated from nonsporulating aa and alpha-alpha parent strains, in which sporulation appeared to be uncoupled from control by mating type. The characterization of one of these mutants (CSP1) is now extended to other processes controlled by mating type. This mutant is indistinguishable from alpha-alpha cells and unlike aalpha cells for mating factor production and response, zygote formation, intragenic mitotic recombination, and for X-ray sensitivity. The mutant apparently undergoes a full round of DNA synthesis in sporulation medium, but with delayed kinetics. Only 20% of the cells complete sporulation. Among spores in completed asci, the frequency of both intra- and intergenic recombination is the same as it is for spores produced by aalpha cells. However, experiments in which cells were shifted from sporulation medium back to minimal growth medium gave a frequency of meiotic recombination between ade2 or leu2 heteroalleles only 25% to 29% as high for CSP1 alpha-alpha diploid or CSP1 aa disomic cells as for aalpha diploid or disomic cells. Because the latter result, indicating recombination defectiveness, measured recombinant production in the entire cell population, whereas the result indicating normal recombination sampled only completed spores, we infer that all meiotic recombination events occuring in the population of CSP1 alpha-alpha cells are concentrated in those few cells which complete sporulation. This high degree of correlation between meiotic recombination and the completion of meiosis and sporulation suggests that recombination may be required for proper meiotic chromosome segregation in yeast just as it appears to be in maize and in Drosophila."}
{"id": "PMID:1093938", "title": "Mutation of a heterothallic strain to homothallism.", "content": "Upon mutagenesis, a heterothallic alpha-alpha diploit strain mutated to homothallism. The gene confering homothallism is nuclear, recessive, and unlinked to mating type. This gene is not allelic to the HO gene, which is responsible for previously described instances of homothallism in yeast. We have designated this new gene for homothallism as cmt (change of mating type).", "contents": "Mutation of a heterothallic strain to homothallism. Upon mutagenesis, a heterothallic alpha-alpha diploit strain mutated to homothallism. The gene confering homothallism is nuclear, recessive, and unlinked to mating type. This gene is not allelic to the HO gene, which is responsible for previously described instances of homothallism in yeast. We have designated this new gene for homothallism as cmt (change of mating type)."}
{"id": "PMID:1093939", "title": "Selection for a large genetic duplication in Salmonella typhimurium.", "content": "Salmonella typhimurium strains containing a duplication of nearly a third of the genome have been isolated by a simple procedure involving selection for improved utilization of L-malate as sole carbon source. The duplication occurs at a very high spontaneous frequency. Strains containing the duplication can be isolated selectively on malate medium, or by a non-selective procedure involving Hfr conjugation. When strains containing the duplication are maintained on non-selective medium, the duplication is readily lost. Genetic evidence suggests that the duplication is chromosomal and tandem. The fact that the recA gene is included in the duplication has been used to obtain evidence that the recA1 marker is recessive to its wild-type allele. Unlike tandem duplications previously described in I. coli, the duplication described in this report appears to have unique endpoints.", "contents": "Selection for a large genetic duplication in Salmonella typhimurium. Salmonella typhimurium strains containing a duplication of nearly a third of the genome have been isolated by a simple procedure involving selection for improved utilization of L-malate as sole carbon source. The duplication occurs at a very high spontaneous frequency. Strains containing the duplication can be isolated selectively on malate medium, or by a non-selective procedure involving Hfr conjugation. When strains containing the duplication are maintained on non-selective medium, the duplication is readily lost. Genetic evidence suggests that the duplication is chromosomal and tandem. The fact that the recA gene is included in the duplication has been used to obtain evidence that the recA1 marker is recessive to its wild-type allele. Unlike tandem duplications previously described in I. coli, the duplication described in this report appears to have unique endpoints."}
{"id": "PMID:1093947", "title": "A controlled, randomized trial of highly selective vagotomy versus selective vagotomy and pyloroplasty in the treatment of duodenal ulcer.", "content": "The results of highly selective vagotomy without drainage and selective vagotomy with pyloroplasty for duodenal ulcer were compared in a randomized, controlled trial of a series of 100 patients. The frequency of dumping, diarrhoea, and epigastric fullness was significantly lower after highly selective (6, 6, and 8 percent) than after selective vagotomy (30, 20, and 28 percent) one year after the operations. Recurrent and persisting duodenal ulcers appearing from one to four years after the operations were significantly more frequent after highly selective (22 percent) than after selective vagotomy (8 percent). No significant relationships were found between recurrent ulceration and gastric acid secretion measurements after the two operations. The Hollander response was early positive in 28 percent and late positive in 30 percent of the patients subjected to highly selective vagotomy, while the corresponding figures after selective vagotomy were 26 and 32 percent. The overall clinical results of the two operations were not different according to the classification of Visick. Excluding the patients with recurrence resulted in significantly better clinical results after highly selective vagotomy.", "contents": "A controlled, randomized trial of highly selective vagotomy versus selective vagotomy and pyloroplasty in the treatment of duodenal ulcer. The results of highly selective vagotomy without drainage and selective vagotomy with pyloroplasty for duodenal ulcer were compared in a randomized, controlled trial of a series of 100 patients. The frequency of dumping, diarrhoea, and epigastric fullness was significantly lower after highly selective (6, 6, and 8 percent) than after selective vagotomy (30, 20, and 28 percent) one year after the operations. Recurrent and persisting duodenal ulcers appearing from one to four years after the operations were significantly more frequent after highly selective (22 percent) than after selective vagotomy (8 percent). No significant relationships were found between recurrent ulceration and gastric acid secretion measurements after the two operations. The Hollander response was early positive in 28 percent and late positive in 30 percent of the patients subjected to highly selective vagotomy, while the corresponding figures after selective vagotomy were 26 and 32 percent. The overall clinical results of the two operations were not different according to the classification of Visick. Excluding the patients with recurrence resulted in significantly better clinical results after highly selective vagotomy."}
{"id": "PMID:1093948", "title": "Pancreatitis after renal transplantation.", "content": "Pancreatitis is seldom seen as a severe complication of renal transplantation. In a review on 1321 renal transplants, 23 cases with 12 deaths are reported (Johnson and Nabseth, 1970). Single case reports may be added. In our departments pancreatitis has proved to be a fairly frequent complication. It developed in 10 (7 percent) of 147 patients with renal transplantation one week to seven and a half years after transplantation (patients with primary hyperparathyroidism excluded). Three of the eight acute cases had haemorrhagic pancreatitis, in two of them leading to death. Two patients had chronic calcifying pancreatitis. Pancreatitis was complicated in one case by abscess formation and in two by severe haemorrhage into a pseudo-cyst. In two patients the diagnosis was made at necropsy only and death was probably not related to the acute pancreatitis. The exact pathogenesis of pancreatitis after renal transplantation cannot be precisely assessed. Possible contributing factors are treatment with corticosteroids, azathioprin, and L-asparaginase, early hypercalcaemia after transplantation, surgery, infections of bacterial or viral origin, and unknown immunological processes.", "contents": "Pancreatitis after renal transplantation. Pancreatitis is seldom seen as a severe complication of renal transplantation. In a review on 1321 renal transplants, 23 cases with 12 deaths are reported (Johnson and Nabseth, 1970). Single case reports may be added. In our departments pancreatitis has proved to be a fairly frequent complication. It developed in 10 (7 percent) of 147 patients with renal transplantation one week to seven and a half years after transplantation (patients with primary hyperparathyroidism excluded). Three of the eight acute cases had haemorrhagic pancreatitis, in two of them leading to death. Two patients had chronic calcifying pancreatitis. Pancreatitis was complicated in one case by abscess formation and in two by severe haemorrhage into a pseudo-cyst. In two patients the diagnosis was made at necropsy only and death was probably not related to the acute pancreatitis. The exact pathogenesis of pancreatitis after renal transplantation cannot be precisely assessed. Possible contributing factors are treatment with corticosteroids, azathioprin, and L-asparaginase, early hypercalcaemia after transplantation, surgery, infections of bacterial or viral origin, and unknown immunological processes."}
{"id": "PMID:1093960", "title": "[Effects of 5-(3-tert-butylamino-2-hydroxy) propoxy-3,4-dihydrocarbostyril hydrochloride(OPC-1085) on coronary circulation and myocardial metabolism].", "content": "Effects of a new beta-adrenergic blocking agent, 5-(3-tert-butylamino-2-hydroxy) propoxy-3,4-dihydrocarbostyril hydrochloride (OPC-1085), on the coronary circulation  and myocardial metabolism were investigated in anesthetized open-chest dogs and isolated perfused dog hearts. In anesthetized open-chest dogs, OPC-1085 antagonized the responses to isoproterenol of heart rate, Vmax, mean blood pressure, myocardial oxygen consumption, coronary blood flow and redox potential. The antagonistic potency of OPC-1085 was stronger than propranolol. OPC-1085 3 to 30 mug/kg caused appreciable decreases, but in doses of 100 to 1,000 mug/kg caused increases in heart rate and Vmax. The effect of OPC-1085 on max dp/dt was similar to that on Vmax. However, propranolol 3 to 3,000 mu-g/kg caused only decreases in heart rate, Vmax and max dp/dt. OPC-1085 3,10 mu-g/kg while propranolol 30, 100 mu-g/kg caused a fall in myocardial oxygen consumption and coronary blood flow. In isolated perfused hearts, intracoronary injection of OPC-1085 0.1 mg almost completely suppressed isoproterenol-induced augmentation of heart rate, myocardial contractile force and coronary blood flow and reduction of redox potential. OPC-1085 0.1 mg caused slight increases in heart rate, myocardial contractile force and myocardial oxygen consumption. It is concluded that OPC-1085 is a more potent beta-adrenergic blocking agent than propranolol and possesses a weak negative inotropic effect in doses of 3 to 30 mu-g/kg and a intrinsic sympathomimetic activity in doses of 100 to 1,000 mu-g/kg.", "contents": "[Effects of 5-(3-tert-butylamino-2-hydroxy) propoxy-3,4-dihydrocarbostyril hydrochloride(OPC-1085) on coronary circulation and myocardial metabolism]. Effects of a new beta-adrenergic blocking agent, 5-(3-tert-butylamino-2-hydroxy) propoxy-3,4-dihydrocarbostyril hydrochloride (OPC-1085), on the coronary circulation  and myocardial metabolism were investigated in anesthetized open-chest dogs and isolated perfused dog hearts. In anesthetized open-chest dogs, OPC-1085 antagonized the responses to isoproterenol of heart rate, Vmax, mean blood pressure, myocardial oxygen consumption, coronary blood flow and redox potential. The antagonistic potency of OPC-1085 was stronger than propranolol. OPC-1085 3 to 30 mug/kg caused appreciable decreases, but in doses of 100 to 1,000 mug/kg caused increases in heart rate and Vmax. The effect of OPC-1085 on max dp/dt was similar to that on Vmax. However, propranolol 3 to 3,000 mu-g/kg caused only decreases in heart rate, Vmax and max dp/dt. OPC-1085 3,10 mu-g/kg while propranolol 30, 100 mu-g/kg caused a fall in myocardial oxygen consumption and coronary blood flow. In isolated perfused hearts, intracoronary injection of OPC-1085 0.1 mg almost completely suppressed isoproterenol-induced augmentation of heart rate, myocardial contractile force and coronary blood flow and reduction of redox potential. OPC-1085 0.1 mg caused slight increases in heart rate, myocardial contractile force and myocardial oxygen consumption. It is concluded that OPC-1085 is a more potent beta-adrenergic blocking agent than propranolol and possesses a weak negative inotropic effect in doses of 3 to 30 mu-g/kg and a intrinsic sympathomimetic activity in doses of 100 to 1,000 mu-g/kg."}
{"id": "PMID:1093965", "title": "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals.", "content": "The protein-mapping method which combines isoelectric focusing in acrylamide gel and gel electrophoresis was previously used mainly for the separation of plant proteins and human serum proteins. We investigated with this technique soluble proteins of mouse tissues (whole embryos, the liver of fetal and adult mice, kidneys) and the proteins of mouse serum. The technique was tested under a number of different conditions to find those best for our purpose; they may represent some general improvements in the method. The protein patterns show high resolution and excellent reproducibility. About 275 spots were found for fetal liver, about 230 for whole embryos (day 14 p.c.) and about 100 for serum. The fact that a high number of protein spots can be evaluated by a single and comparatively simple experiment suggests that this method may be useful as an assay system for induced point mutations. The protein patterns demonstrated are compared and discgs of dominant lethal examinations after acute and subacute application of these three substances.", "contents": "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals. The protein-mapping method which combines isoelectric focusing in acrylamide gel and gel electrophoresis was previously used mainly for the separation of plant proteins and human serum proteins. We investigated with this technique soluble proteins of mouse tissues (whole embryos, the liver of fetal and adult mice, kidneys) and the proteins of mouse serum. The technique was tested under a number of different conditions to find those best for our purpose; they may represent some general improvements in the method. The protein patterns show high resolution and excellent reproducibility. About 275 spots were found for fetal liver, about 230 for whole embryos (day 14 p.c.) and about 100 for serum. The fact that a high number of protein spots can be evaluated by a single and comparatively simple experiment suggests that this method may be useful as an assay system for induced point mutations. The protein patterns demonstrated are compared and discgs of dominant lethal examinations after acute and subacute application of these three substances."}
{"id": "PMID:1093966", "title": "Experimental Escherichia coli O6 pyelonephritis in rabbits. Effect on O6 antibody quantity and avidity of prior immunization with E. coli O2 bacteria.", "content": "Haematogenous pyelonephritis was induced in rabbits using Escherichia coli 06:K13:H1 bacteria and the amounts and avidities of antibodies to the 06 antigen were analysed by the ammonium sulphate precipitation technique of Farr. In a group of six animals preimmunized with E. coli 02:K2ab:H1, five developed pyelonephritis and one pyelitis, as determined by histological examination. All aminals showed a considerable antibody response to E. coli 06 antigen during the infection. The animal with pyelitis gave a slightly smaller response than the others. The antibody avidity showed a pronounced variation. In a second group of six rabbits not preimmunized, five animals developed pyelonephritis. The titres of antibodies against E. coli 06 antigen increased during the infection inall of the six animals. However, the increase was significantly smaller than for the animals preimmunized with E. coli 02:K2ab:H1 (P smaller than 0.01). The pattern of the antibody avidities in this group was also heterogenous. The results are consistent with previous findings that exposure to serologically heterologous E. coli bacteria can enhance the development of the homologous antibody titres. This could be of relevance for serological diagnostic work as well as in the determination of the protective capacity of the antibody.", "contents": "Experimental Escherichia coli O6 pyelonephritis in rabbits. Effect on O6 antibody quantity and avidity of prior immunization with E. coli O2 bacteria. Haematogenous pyelonephritis was induced in rabbits using Escherichia coli 06:K13:H1 bacteria and the amounts and avidities of antibodies to the 06 antigen were analysed by the ammonium sulphate precipitation technique of Farr. In a group of six animals preimmunized with E. coli 02:K2ab:H1, five developed pyelonephritis and one pyelitis, as determined by histological examination. All aminals showed a considerable antibody response to E. coli 06 antigen during the infection. The animal with pyelitis gave a slightly smaller response than the others. The antibody avidity showed a pronounced variation. In a second group of six rabbits not preimmunized, five animals developed pyelonephritis. The titres of antibodies against E. coli 06 antigen increased during the infection inall of the six animals. However, the increase was significantly smaller than for the animals preimmunized with E. coli 02:K2ab:H1 (P smaller than 0.01). The pattern of the antibody avidities in this group was also heterogenous. The results are consistent with previous findings that exposure to serologically heterologous E. coli bacteria can enhance the development of the homologous antibody titres. This could be of relevance for serological diagnostic work as well as in the determination of the protective capacity of the antibody."}
{"id": "PMID:1093967", "title": "Age-related decline in the antibody response to E. coli lipopolysaccharide in New Zealand Black mice.", "content": "A thymus-independent immune function in ageing NZB and BALB/c mice was compared by measuring the antibody response to E. coli lipopolysaccharide (LPS). Since it was found that 10-13 month-old NZB mice was particularly sensitive to LPS, this antigen had to be detoxified by alkali treatment. The anti-LPS splenic plaque-forming cell (PFC) response of NZB mice decreases with age and was lower than that of BALB/c mice in all age groups studied. The response of 4- and 10-month-old NZB mice showed an irregular time course and a number of mice showed no response. The present results indicate that, besides an impairment of T-cell functions, an impairment on the B-cell level must also be considered in ageing NZB mice.", "contents": "Age-related decline in the antibody response to E. coli lipopolysaccharide in New Zealand Black mice. A thymus-independent immune function in ageing NZB and BALB/c mice was compared by measuring the antibody response to E. coli lipopolysaccharide (LPS). Since it was found that 10-13 month-old NZB mice was particularly sensitive to LPS, this antigen had to be detoxified by alkali treatment. The anti-LPS splenic plaque-forming cell (PFC) response of NZB mice decreases with age and was lower than that of BALB/c mice in all age groups studied. The response of 4- and 10-month-old NZB mice showed an irregular time course and a number of mice showed no response. The present results indicate that, besides an impairment of T-cell functions, an impairment on the B-cell level must also be considered in ageing NZB mice."}
{"id": "PMID:1093968", "title": "Leucoagglutination and cytotoxicity of the serum of infected mice and of extracts of Trichinella spiralis larvae and the capacity of infected mouse sera to prolong skin allografts.", "content": "The sera of mice infected with the nematode Trichinella spiralis agglutinate and kill homologous lymphoid cells in vitro. The agglutinating activity is present in the sera of infected animals on the 7th day following inoculation with the parasite, rises to a maximum on the 30th day of the infection and then decreases. The leucoagglutinating titre of these sera is related neither to the level of the inoculating dose of parasites nor to the intensity of muscle infection. The agglutinating and leucotoxic activity of the sera of infected mice is probably due to the same factor(s), but target cells require sensitization before cytotoxicity becomes apparent. Skin allografts were prolonged in infected animals or in animals treated with the serum of infected animals. Saline extracts of T. spiralis larvae also have leucoagglutinating and leucotoxic activity.", "contents": "Leucoagglutination and cytotoxicity of the serum of infected mice and of extracts of Trichinella spiralis larvae and the capacity of infected mouse sera to prolong skin allografts. The sera of mice infected with the nematode Trichinella spiralis agglutinate and kill homologous lymphoid cells in vitro. The agglutinating activity is present in the sera of infected animals on the 7th day following inoculation with the parasite, rises to a maximum on the 30th day of the infection and then decreases. The leucoagglutinating titre of these sera is related neither to the level of the inoculating dose of parasites nor to the intensity of muscle infection. The agglutinating and leucotoxic activity of the sera of infected mice is probably due to the same factor(s), but target cells require sensitization before cytotoxicity becomes apparent. Skin allografts were prolonged in infected animals or in animals treated with the serum of infected animals. Saline extracts of T. spiralis larvae also have leucoagglutinating and leucotoxic activity."}
{"id": "PMID:1093969", "title": "The effect of lysozyme on the complement-dependent bactericidal action of different antibody classes.", "content": "Preparations of rabbit, dog and sheep IgA, IgA and IgM were examined for their antibacterial effects using a complement-dependent bactericidal assay. IgM and IgG were efficient bactericidal antibodies in the presence of complement; IgA, however, contained negligible activity. Except for sheep IgG no enhancement of bactericidal activity was observed in the presence of added lysozyme.", "contents": "The effect of lysozyme on the complement-dependent bactericidal action of different antibody classes. Preparations of rabbit, dog and sheep IgA, IgA and IgM were examined for their antibacterial effects using a complement-dependent bactericidal assay. IgM and IgG were efficient bactericidal antibodies in the presence of complement; IgA, however, contained negligible activity. Except for sheep IgG no enhancement of bactericidal activity was observed in the presence of added lysozyme."}
{"id": "PMID:1093970", "title": "Use of the defined antigen substrate spheres system as a model for analysing possible mechanisms of inhibition-blockade of anti-tumour lymphocytotoxicity.", "content": "The defined antigen substrate spheres (DASS) system was employed for analysing reactions between solid state antibody or antigen and soluble immune complexes. Sepharose beads covalently coupled with ovalbumin were used to represent tumour cells and beads coupled with antibody against ovalbumin were used to represent anti-tumour lymphocytes; the ovalbumin and corresponding antibody simulated tumour-derived antigen and antibody to tumour respectively. Binding of soluble complexes to the beads was measured by fluorimetry and/or radiometry of fluorescein or 125-I-labelled ovalbumin or antibody. Antigen-antibody complexes in antibody excess bound less effectively to the antibody beads than antigen alone, but complexes in slight or moderate antigen excess bound more effectively. Complexes in antibody excess were most effective in the complex before levelling off and then decreased in extreme antibody excess. The model demonstration of augmentation by antibody of antigen binding to solid state antibody might by analogy reflect a mechanism of inhibition of lymphocyte cytotoxicity. Complexes in a wide range of antibody excess should also be effective in blocking lymphocytotoxicity at the target cell level.", "contents": "Use of the defined antigen substrate spheres system as a model for analysing possible mechanisms of inhibition-blockade of anti-tumour lymphocytotoxicity. The defined antigen substrate spheres (DASS) system was employed for analysing reactions between solid state antibody or antigen and soluble immune complexes. Sepharose beads covalently coupled with ovalbumin were used to represent tumour cells and beads coupled with antibody against ovalbumin were used to represent anti-tumour lymphocytes; the ovalbumin and corresponding antibody simulated tumour-derived antigen and antibody to tumour respectively. Binding of soluble complexes to the beads was measured by fluorimetry and/or radiometry of fluorescein or 125-I-labelled ovalbumin or antibody. Antigen-antibody complexes in antibody excess bound less effectively to the antibody beads than antigen alone, but complexes in slight or moderate antigen excess bound more effectively. Complexes in antibody excess were most effective in the complex before levelling off and then decreased in extreme antibody excess. The model demonstration of augmentation by antibody of antigen binding to solid state antibody might by analogy reflect a mechanism of inhibition of lymphocyte cytotoxicity. Complexes in a wide range of antibody excess should also be effective in blocking lymphocytotoxicity at the target cell level."}
{"id": "PMID:1093971", "title": "In vitro synthesis of lysozyme by human and mouse tissues and leucocytes.", "content": "Previous studies have shown that lysozyme can be detected in many body fluids, in extracts of tissues, and also in granulocytes, monocytes and macrophages. However, the sites of synthesis of lysozyme have not been defined. In the present report, the synthesis of lysozyme by tissues, and by defined cell populations cultured in vitro has been studied by detecting the incorporation of 14-C-labelled amino acids into lysozyme. This method detects only lysozyme newly synthesized during the incubation of the specimen and therefore shows unequivocally which tissues and cell types are capable of lysozyme synthesis. The validity of the method has been shown by parallel studies using an independent method to detect lysozyme production in vitro. In studies in humans and mice, lysozyme synthesis has been demonstrated in the mucosa of the respiratory and gastrointestinal tracts, and in lymphoid organs. In studies of defined cell populations, monocytes and macrophages (mononuclear phagocytes) have been shown to synthesize lysozyme. Granulocytes from peripheral blood contain lysozyme but do not synthesize it, and lymphocytes neither contain nor synthesize lysozyme. The present findings provide further evidence that lysozyme has an important role in the defence of the host against micro-organisms, and the findings suggest that lysozyme may reach its target by several routes. At an intracellular level it is delivered from lysosomes into the phagocytic granules of granulocytes and macrophages. Local synthesis of the mucous membranes contributes lysozyme to secretions. Synthesis and secretion by mononuclear phagocytes which reach a tissue in response to an inflammatory stimulus contribute lysozyme to the exudate, and the release of lysozyme from breakdown of granulocytes has the same effect.", "contents": "In vitro synthesis of lysozyme by human and mouse tissues and leucocytes. Previous studies have shown that lysozyme can be detected in many body fluids, in extracts of tissues, and also in granulocytes, monocytes and macrophages. However, the sites of synthesis of lysozyme have not been defined. In the present report, the synthesis of lysozyme by tissues, and by defined cell populations cultured in vitro has been studied by detecting the incorporation of 14-C-labelled amino acids into lysozyme. This method detects only lysozyme newly synthesized during the incubation of the specimen and therefore shows unequivocally which tissues and cell types are capable of lysozyme synthesis. The validity of the method has been shown by parallel studies using an independent method to detect lysozyme production in vitro. In studies in humans and mice, lysozyme synthesis has been demonstrated in the mucosa of the respiratory and gastrointestinal tracts, and in lymphoid organs. In studies of defined cell populations, monocytes and macrophages (mononuclear phagocytes) have been shown to synthesize lysozyme. Granulocytes from peripheral blood contain lysozyme but do not synthesize it, and lymphocytes neither contain nor synthesize lysozyme. The present findings provide further evidence that lysozyme has an important role in the defence of the host against micro-organisms, and the findings suggest that lysozyme may reach its target by several routes. At an intracellular level it is delivered from lysosomes into the phagocytic granules of granulocytes and macrophages. Local synthesis of the mucous membranes contributes lysozyme to secretions. Synthesis and secretion by mononuclear phagocytes which reach a tissue in response to an inflammatory stimulus contribute lysozyme to the exudate, and the release of lysozyme from breakdown of granulocytes has the same effect."}
{"id": "PMID:1093972", "title": "Inhibition of T-cell differentiation by an antibody to a soluble thymic factor.", "content": "An antiserum to chicken soluble thymic factor (STF) was prepared in rabbits and absorbed with chicken thymocytes until it reacted only with thymic reticuloepithelial cells. When injected into 6-day-old embryonated eggs, this antiserum caused a severe depletion of small lymphocytes in the cortical region of the thymus which was filled with large undifferentiated cells. In addition, reticuloepithelial cells displayed a marked disruption of cytoplasmic elements. The percentage of thymic lymphocytes bearing the T antigen was greatly reduced. It is believed that a factor responsible for differentiation of stem cells into T cells has been inhibited.", "contents": "Inhibition of T-cell differentiation by an antibody to a soluble thymic factor. An antiserum to chicken soluble thymic factor (STF) was prepared in rabbits and absorbed with chicken thymocytes until it reacted only with thymic reticuloepithelial cells. When injected into 6-day-old embryonated eggs, this antiserum caused a severe depletion of small lymphocytes in the cortical region of the thymus which was filled with large undifferentiated cells. In addition, reticuloepithelial cells displayed a marked disruption of cytoplasmic elements. The percentage of thymic lymphocytes bearing the T antigen was greatly reduced. It is believed that a factor responsible for differentiation of stem cells into T cells has been inhibited."}
{"id": "PMID:1093973", "title": "T cell-dependent mediator and B-cell clones.", "content": "Supernatants were collected from keyhole limpet haemocyanin (KLH) primed spleen cells which had been incubated in tissue culture medium with their priming antigen KLH. These non-specific factor (NSF) containing supernatants were then tested in a microculture system for their ability to facilitate an anti-SRBC response of nu/nu or AT times BM spleen cells. It was concluded that:(a) NSF was able to engage a large number of the available pool of sheep erythrocyte (SRBC) specific B cells into an antibody response (b) this response was characterized by the development of clones expressing plaque-forming cells (PFC), the number of PFC produced within a clone being dependent upon the amount of NSF available in that culture; (c) NSF probably acted directly on B cells, and not via other accessory cell types.", "contents": "T cell-dependent mediator and B-cell clones. Supernatants were collected from keyhole limpet haemocyanin (KLH) primed spleen cells which had been incubated in tissue culture medium with their priming antigen KLH. These non-specific factor (NSF) containing supernatants were then tested in a microculture system for their ability to facilitate an anti-SRBC response of nu/nu or AT times BM spleen cells. It was concluded that:(a) NSF was able to engage a large number of the available pool of sheep erythrocyte (SRBC) specific B cells into an antibody response (b) this response was characterized by the development of clones expressing plaque-forming cells (PFC), the number of PFC produced within a clone being dependent upon the amount of NSF available in that culture; (c) NSF probably acted directly on B cells, and not via other accessory cell types."}
{"id": "PMID:1093974", "title": "Binding of aggregated human immunoglobulin to murine thymocytes and T cells through receptors for the Fc region.", "content": "Normal thymus lymphocytes and T cells of mice have the ability to bind heat-aggregated IgG of human origin (aggHIgG), as shown by indirect immunofluorescence. At 4 degrees, the cells bind aggHIgG with an irregular speckled appearance; at 37 degrees, the aggregates are incorporated into the surface membrane, inducing rearrangement of the receptors and capping. At the point of maximum binding capacity, thymocytes show a fairly homogeneous fluorescence pattern, whereas T cells show a heterogeneous appearance. Aggregated pure human Fc fragments, but not Fab fragments, retained not only the binding capacity of the complete molecule but also the ability to induce cap formation on the surface of thymusceds, at 37 degrees.", "contents": "Binding of aggregated human immunoglobulin to murine thymocytes and T cells through receptors for the Fc region. Normal thymus lymphocytes and T cells of mice have the ability to bind heat-aggregated IgG of human origin (aggHIgG), as shown by indirect immunofluorescence. At 4 degrees, the cells bind aggHIgG with an irregular speckled appearance; at 37 degrees, the aggregates are incorporated into the surface membrane, inducing rearrangement of the receptors and capping. At the point of maximum binding capacity, thymocytes show a fairly homogeneous fluorescence pattern, whereas T cells show a heterogeneous appearance. Aggregated pure human Fc fragments, but not Fab fragments, retained not only the binding capacity of the complete molecule but also the ability to induce cap formation on the surface of thymusceds, at 37 degrees."}
{"id": "PMID:1093975", "title": "Immune response in aged and young subjects, following administration of large doses of tuberculin.", "content": "Different parameters of the immunologic response have been studied in young and aged subjects before, during and after administration of increasing doses of tuberculin purified protein derivative (PPD). In both age groups, a considerable increase in skin reaction was observed with the progression of antigen administration. This is particularly striking among old subjects, who before the treatment had either no reaction to tuberculin or a very weak one. Increased production of circulating antibodies to tuberculin was also consistently observed. The treatment resulted, especially in the old patients, in an increase in the lymphocyte sensitivity to phytohemagglutinin. The production of migration inhibitory factors was studied with lymphocytes from 6 treated subjects, in all cases this production was of the same degree as the one obtained from lymphocytes of PPD-positive untreated subjects. Lymphocyte cultures done during the treatment showed an absence of response in the presence of tuberculin, and in young patients only a slight increase in the spontaneous synthesis of desoxyribonucleic acid in the absence of tuberculin. These results demonstrate that in old people the depression of cellular immunity seems to be small. Adequate and prolonged antigenic stimuli are able to reactivate to approximately normal levels many features of this altered function.", "contents": "Immune response in aged and young subjects, following administration of large doses of tuberculin. Different parameters of the immunologic response have been studied in young and aged subjects before, during and after administration of increasing doses of tuberculin purified protein derivative (PPD). In both age groups, a considerable increase in skin reaction was observed with the progression of antigen administration. This is particularly striking among old subjects, who before the treatment had either no reaction to tuberculin or a very weak one. Increased production of circulating antibodies to tuberculin was also consistently observed. The treatment resulted, especially in the old patients, in an increase in the lymphocyte sensitivity to phytohemagglutinin. The production of migration inhibitory factors was studied with lymphocytes from 6 treated subjects, in all cases this production was of the same degree as the one obtained from lymphocytes of PPD-positive untreated subjects. Lymphocyte cultures done during the treatment showed an absence of response in the presence of tuberculin, and in young patients only a slight increase in the spontaneous synthesis of desoxyribonucleic acid in the absence of tuberculin. These results demonstrate that in old people the depression of cellular immunity seems to be small. Adequate and prolonged antigenic stimuli are able to reactivate to approximately normal levels many features of this altered function."}
{"id": "PMID:1093976", "title": "Purified antibodies to collagen: an immunofluorescence study of their reaction with tissue collagen.", "content": "Antibodies to soluble calf or rat skin collagen were purified by appropriate immunoadsorption and separated into three subfractions directed either to antigenic sites on unfolded alpha-chains (denatured collagen), to nonhelical sites, or to helical sites exposed on the triple helical molecule. In indirect immunofluorescence tests each of the antibody solutions reacted with collagen of skin and kidney tissue, although the latter two antibody solutions appeared to be more active. Distinct activity was also observed with antibodies to the N-terminal antigenic determinant of rat skin collagen alpha2-chain, a structure usually involved in cross-linking. Indirect immunofluorescence tests with anti-collagen sera on sections of skin resulted in the staining of the whole dermis, while anti-procollagen sera revealed binding only to the uppermost subepithelial layer of the dermis (stratum papillare). On kidney sections only the interstitial connective tissue reacted with purified anti-collagen or anti-procollagen sera. Both skin and glomerular basement membranes remained unstained with either kind of purified antibodies. However, antisera not subjected to immunoadsorption do react with the glomerular basement membrane. Antibodies to noncollagenous contaminants are considered to be responsible for this finding which emphasizes the necessity to use purified antibodies exclusively for this type of immunofluorescence analysis.", "contents": "Purified antibodies to collagen: an immunofluorescence study of their reaction with tissue collagen. Antibodies to soluble calf or rat skin collagen were purified by appropriate immunoadsorption and separated into three subfractions directed either to antigenic sites on unfolded alpha-chains (denatured collagen), to nonhelical sites, or to helical sites exposed on the triple helical molecule. In indirect immunofluorescence tests each of the antibody solutions reacted with collagen of skin and kidney tissue, although the latter two antibody solutions appeared to be more active. Distinct activity was also observed with antibodies to the N-terminal antigenic determinant of rat skin collagen alpha2-chain, a structure usually involved in cross-linking. Indirect immunofluorescence tests with anti-collagen sera on sections of skin resulted in the staining of the whole dermis, while anti-procollagen sera revealed binding only to the uppermost subepithelial layer of the dermis (stratum papillare). On kidney sections only the interstitial connective tissue reacted with purified anti-collagen or anti-procollagen sera. Both skin and glomerular basement membranes remained unstained with either kind of purified antibodies. However, antisera not subjected to immunoadsorption do react with the glomerular basement membrane. Antibodies to noncollagenous contaminants are considered to be responsible for this finding which emphasizes the necessity to use purified antibodies exclusively for this type of immunofluorescence analysis."}
{"id": "PMID:1093977", "title": "Changes for Bic\u00eatre and its elderly residents: the paradox of progress.", "content": "Recent changes at Bic\u00eatre, the historic French institution, exemplify an old paradox in the care of the elderly: improvements which benefit part of a society can mean more misfortunes for others.  The effect of relocation of its elderly residents to make way for renovations poses important questions of priorities in societal values.", "contents": "Changes for Bic\u00eatre and its elderly residents: the paradox of progress. Recent changes at Bic\u00eatre, the historic French institution, exemplify an old paradox in the care of the elderly: improvements which benefit part of a society can mean more misfortunes for others.  The effect of relocation of its elderly residents to make way for renovations poses important questions of priorities in societal values."}
{"id": "PMID:1093983", "title": "Spread of Trichophyton mentagrophytes var. gran. infection to man.", "content": "The occurrence of T. mentagrophytes var. gran. infection of small mammals in their natural habitat was studied. The most common host of the exoanthropic mammals was Clethrionomys glareolus (bank vole); the next most common hosts were hemisynanthropes Sorex araneus (common shrew) and eusynathropes Mus musculus (common mouse). Occurrence of infection increased during the winter months. Because small mammals gather in farm outbuilding during cold weather, man is more likely to be exposed to infection from them during this time. Infection from T. mentagrophytes var. gan. is more likely to occur among agricultural workers than among others. The type of work influences the location of skin infection.", "contents": "Spread of Trichophyton mentagrophytes var. gran. infection to man. The occurrence of T. mentagrophytes var. gran. infection of small mammals in their natural habitat was studied. The most common host of the exoanthropic mammals was Clethrionomys glareolus (bank vole); the next most common hosts were hemisynanthropes Sorex araneus (common shrew) and eusynathropes Mus musculus (common mouse). Occurrence of infection increased during the winter months. Because small mammals gather in farm outbuilding during cold weather, man is more likely to be exposed to infection from them during this time. Infection from T. mentagrophytes var. gan. is more likely to occur among agricultural workers than among others. The type of work influences the location of skin infection."}
{"id": "PMID:1093984", "title": "Clinical evaluation of desoximetasone in treatment of dermatoses and psoriasis.", "content": "A comparative clinical evaluation of desoximetasone, a corticosterone derivative by a double blind study showed that desoximetasone has potent topical activity as effective as betamethasone valerate and probably more effective than triamcinolone acetonide.", "contents": "Clinical evaluation of desoximetasone in treatment of dermatoses and psoriasis. A comparative clinical evaluation of desoximetasone, a corticosterone derivative by a double blind study showed that desoximetasone has potent topical activity as effective as betamethasone valerate and probably more effective than triamcinolone acetonide."}
{"id": "PMID:1093986", "title": "Studies of the mechanisms of radiosensitization of bacterial and mammalian cells by Diamide.", "content": "Diamide sensitizes bacterial and mammalian cells to radiation by at least two mechanisms. Sensitization of V79-GL1 Chinese Hamster cells is due mainly to a reduction of a reduction of the survival-curve shoulder, is observed both in oxygen and in hypoxia,and is additive to the sensitization of hypoxic cells by some nitroimidazoles. In contrast, sensitization of the radioresistant organism, Micrococcus sodonensis, which has apronounced shoulder, is entirely dose-modifying. In a rapid-mix study using Serratia marcescens, two mechanisms of sensitization have been identified. After 4 to 40 msec preirradiation contact with Daimide, the enhancement ratio is constant at 1.6; after 10min contact, it is 2.5. With V79-GL1 cells, no sensitization occurs in the short-time-range; likewise non is seen when Diamide is added to bacteria or mammalian cells 4 msec after irradiation. It is concluded that Diamide sensitizes by at least two mechanisms, one of which resembles that of the electron-affinic type.", "contents": "Studies of the mechanisms of radiosensitization of bacterial and mammalian cells by Diamide. Diamide sensitizes bacterial and mammalian cells to radiation by at least two mechanisms. Sensitization of V79-GL1 Chinese Hamster cells is due mainly to a reduction of a reduction of the survival-curve shoulder, is observed both in oxygen and in hypoxia,and is additive to the sensitization of hypoxic cells by some nitroimidazoles. In contrast, sensitization of the radioresistant organism, Micrococcus sodonensis, which has apronounced shoulder, is entirely dose-modifying. In a rapid-mix study using Serratia marcescens, two mechanisms of sensitization have been identified. After 4 to 40 msec preirradiation contact with Daimide, the enhancement ratio is constant at 1.6; after 10min contact, it is 2.5. With V79-GL1 cells, no sensitization occurs in the short-time-range; likewise non is seen when Diamide is added to bacteria or mammalian cells 4 msec after irradiation. It is concluded that Diamide sensitizes by at least two mechanisms, one of which resembles that of the electron-affinic type."}
{"id": "PMID:1094000", "title": "Postoperative monitoring following critical trauma.", "content": "This review has focused on several aspects of monitoring post-injury patients. A few of the more commonly used monitoring devices have been discussed to emphasize that their use carries some risk and, as such, proper indications, based on the benefit to be achieved, must be available before they are used. Guidelines for the safe use of these devices have been outlined. The postoperative injury patient is at risk for developing certain complications as a result of the magnitude of injury. This review has dealt with respiratory insufficiency, renal insufficiency, and stress ulceration. Guidelines have been presented to aid in the prevention, early diagnosis, and management of these problems.", "contents": "Postoperative monitoring following critical trauma. This review has focused on several aspects of monitoring post-injury patients. A few of the more commonly used monitoring devices have been discussed to emphasize that their use carries some risk and, as such, proper indications, based on the benefit to be achieved, must be available before they are used. Guidelines for the safe use of these devices have been outlined. The postoperative injury patient is at risk for developing certain complications as a result of the magnitude of injury. This review has dealt with respiratory insufficiency, renal insufficiency, and stress ulceration. Guidelines have been presented to aid in the prevention, early diagnosis, and management of these problems."}
{"id": "PMID:1094016", "title": "Bone induction in implants of decalcified bone and dentine.", "content": "The fate of decalcified bone and dentine implanted in muscle and beneath the kidney capsule has been studied in young rats. Quantitatively speaking there was a great deal of variation, but in general the implants became surrounded and invaded by young vascular connective tissue; then tunnels were eroded and cavities enlarged by multi-nucleated giant cells; then the matrix around erosion chambers became recalcified; and finally new bone was induced on the eroded recalcified surfaces. Erosion was much more extensive, and bone was much more readily induced in the intramuscular than in the subcapsular implants. It is concluded that the presence of an eroded, recalcified surface is a pre-requisite for bone induction under these conditions.", "contents": "Bone induction in implants of decalcified bone and dentine. The fate of decalcified bone and dentine implanted in muscle and beneath the kidney capsule has been studied in young rats. Quantitatively speaking there was a great deal of variation, but in general the implants became surrounded and invaded by young vascular connective tissue; then tunnels were eroded and cavities enlarged by multi-nucleated giant cells; then the matrix around erosion chambers became recalcified; and finally new bone was induced on the eroded recalcified surfaces. Erosion was much more extensive, and bone was much more readily induced in the intramuscular than in the subcapsular implants. It is concluded that the presence of an eroded, recalcified surface is a pre-requisite for bone induction under these conditions."}
{"id": "PMID:1094020", "title": "Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells.", "content": "An antibody against purified chicken skeletal muscle tropomyosin is used in indirect immunofluorescence to visualize the localization of tropomyosin in a variety of nonmuscle cells. The antibody produces a fluorescent pattern which is very similar to that obtained with an actin-specific antibody. This pattern is composed of fluorescent fibers which are shown to be coincident with the fibers seen with phase-contrast optics. High resolution epifluorescent microscopy reveals that fibers stained with the actin antibody show a continuous fluorescence, while fibers reacted with the tropomyosin antibody show a periodic fluorescence. Measurements indicate that the lengths of the fluorescent segments are variable with an average of 1.2 mum while the spacing between segments is approximately 0.4 mum.", "contents": "Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells. An antibody against purified chicken skeletal muscle tropomyosin is used in indirect immunofluorescence to visualize the localization of tropomyosin in a variety of nonmuscle cells. The antibody produces a fluorescent pattern which is very similar to that obtained with an actin-specific antibody. This pattern is composed of fluorescent fibers which are shown to be coincident with the fibers seen with phase-contrast optics. High resolution epifluorescent microscopy reveals that fibers stained with the actin antibody show a continuous fluorescence, while fibers reacted with the tropomyosin antibody show a periodic fluorescence. Measurements indicate that the lengths of the fluorescent segments are variable with an average of 1.2 mum while the spacing between segments is approximately 0.4 mum."}
{"id": "PMID:1094021", "title": "A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature.", "content": "This paper describes the development of a miniature, temperature-controlled, stainless steel pressure chamber which uses strain-free optical glass for windows. It is directly adaptable to standard phase-contrast and polarized-light microscopes and requires a minimum amount of equipment to generate and measure pressure. Birefringence retardation (BR) og 0.1 nm up to 3,000 psi, 0.4 nm up to 5,000 psi and 1.0 nm up to 10,000 psi can be detected over a 0.75-mm central field with two strain-free Leitz 20 times UM objectives, one used as a condenser. In phase-contrast studies a Nikon DML 40 times phase objective and Zeiss model IS long working-distance phase condenser were used, with little deterioration of image quality or contrast at pressures as high as 12,000 psi. The actual design process required a synthesis of various criteria which may be categorized under four main areas of consideration: (a) specimen physiology; (b) constraints imposed by available optical equipment and standard microscope systems; (c) mechanical strength and methods for generating pressure; and (d) optical requirements of the chamber windows. Procedures for using the chambers, as well as methods for shifting and controlling the temperature within the chamber, are included.", "contents": "A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. This paper describes the development of a miniature, temperature-controlled, stainless steel pressure chamber which uses strain-free optical glass for windows. It is directly adaptable to standard phase-contrast and polarized-light microscopes and requires a minimum amount of equipment to generate and measure pressure. Birefringence retardation (BR) og 0.1 nm up to 3,000 psi, 0.4 nm up to 5,000 psi and 1.0 nm up to 10,000 psi can be detected over a 0.75-mm central field with two strain-free Leitz 20 times UM objectives, one used as a condenser. In phase-contrast studies a Nikon DML 40 times phase objective and Zeiss model IS long working-distance phase condenser were used, with little deterioration of image quality or contrast at pressures as high as 12,000 psi. The actual design process required a synthesis of various criteria which may be categorized under four main areas of consideration: (a) specimen physiology; (b) constraints imposed by available optical equipment and standard microscope systems; (c) mechanical strength and methods for generating pressure; and (d) optical requirements of the chamber windows. Procedures for using the chambers, as well as methods for shifting and controlling the temperature within the chamber, are included."}
{"id": "PMID:1094025", "title": "Production of a comprehensive research directory from multiple secondary sources.", "content": "Printed, automated, and manually maintained sources of information about cancer research and control activities were identified. Data were consolidated into an automated file to characterize the organizations active in such work and to identify individual projects and scientists. A computer-formatted directory was produced, with access via geographic location, personal name, organizational name, and keyword.", "contents": "Production of a comprehensive research directory from multiple secondary sources. Printed, automated, and manually maintained sources of information about cancer research and control activities were identified. Data were consolidated into an automated file to characterize the organizations active in such work and to identify individual projects and scientists. A computer-formatted directory was produced, with access via geographic location, personal name, organizational name, and keyword."}
{"id": "PMID:1094027", "title": "Chromatographic hydrophobic parameters in correlation analysis of structure-activity relationships.", "content": "The use of RM and delta RM parameters as indices of hydrophobicity for inclusion in quantitative structure activity relationships has been studied. The relationship between these parameters and other free-energy related parameters is illustrated theoretically and experimentally. It is suggested that the chromatographically obtained parameters could find a wider applicability in structure-activity relationships, and that their use would result in improved correlation of data.", "contents": "Chromatographic hydrophobic parameters in correlation analysis of structure-activity relationships. The use of RM and delta RM parameters as indices of hydrophobicity for inclusion in quantitative structure activity relationships has been studied. The relationship between these parameters and other free-energy related parameters is illustrated theoretically and experimentally. It is suggested that the chromatographically obtained parameters could find a wider applicability in structure-activity relationships, and that their use would result in improved correlation of data."}
{"id": "PMID:1094028", "title": "Chromatographic analysis of fungicides.", "content": "The separation and analysis of a wide range of fungicides, by gas, liquid, column, paper, and thin-layer chromatography have been reviewed. Major attention has been given to methods for the identification and quantitation of individual and multiresidues of fungicides in environmental and agricultural samples.", "contents": "Chromatographic analysis of fungicides. The separation and analysis of a wide range of fungicides, by gas, liquid, column, paper, and thin-layer chromatography have been reviewed. Major attention has been given to methods for the identification and quantitation of individual and multiresidues of fungicides in environmental and agricultural samples."}
{"id": "PMID:1094029", "title": "Myotonia dystrophica: Studies on gonadal function using luteinizing hormone-releasing hormone (LRH).", "content": "Eight men and four women with myotonia dystrophica were investigated. The gonadotropin responses to 100 mug luteinizing hormone-releasing hormone (LRH) were studied, and serum testosterone assayed. In the men with testicular atrophy a high basal follicle stimulating hormone was found with an exaggerated response to LRH. Although the mean basal luteinizing hormone was not significantly elevated, there was an excessive response to LRH, which was possibly conditioned by the lower mean testoerone levels in this group. This study provides biochemical indices of both the primary nature of the testicular damage and the disproportionately greater involvement of the seminiferous tubules. The pituitary-gonadal axis was normal in the women.", "contents": "Myotonia dystrophica: Studies on gonadal function using luteinizing hormone-releasing hormone (LRH). Eight men and four women with myotonia dystrophica were investigated. The gonadotropin responses to 100 mug luteinizing hormone-releasing hormone (LRH) were studied, and serum testosterone assayed. In the men with testicular atrophy a high basal follicle stimulating hormone was found with an exaggerated response to LRH. Although the mean basal luteinizing hormone was not significantly elevated, there was an excessive response to LRH, which was possibly conditioned by the lower mean testoerone levels in this group. This study provides biochemical indices of both the primary nature of the testicular damage and the disproportionately greater involvement of the seminiferous tubules. The pituitary-gonadal axis was normal in the women."}
{"id": "PMID:1094030", "title": "Human pituitary gonadotropin index. I. Standardized LRH test criteria for evaluation of functional amenorrhea.", "content": "LRH test were carried out by giving 339 amenorrheic women and 74 normally menstruating volunteers an intravenous injection of 25 mug LRH (Hoe 471). Plasma LH and FSH were measured by RIA in two laboratories (Tuebingen and Ulm) using two standard reference preparations: LER 907 and 2nd IRP-HMG. The average conversion factors between the two standard preparations were calculated at 5.0 for LH and 25.0 for FSH. Furthermore, the estradiol-17beta levels were measured in 139 out of the 339 patients immediately before and 60 minutes after LRH injection. Taking the episodic and cyclic plasma gonadotropin fluctuations into consideration a shorthand system classifying the gonadotropin baseline (BI-BIV) and LH responses to 25 mug LRH (R0-R2) has been established and is referred to as Human Pituitary Gonadotropin Index (HPGI). It is possible to achieve reproducible gonadotropin results in two different laboratories using two different standard reference preparations. Two separate, randomly selected groups of amenorrheic women were found to have the same percent distribution of the HPGI. A correlation coefficient of r equal 0.67 between basal and LRH stimulated plasma LH levels does not sufficiently characterize the individual LH response behavior. A significant increase of plasma LH and FSH within the test period (60') reveals that the iv administration of 25 mug LRH represents an adequate dose for the LRH test in women. The HPGI which characterizes the functional state of gonadostat, may become a useful diagnostic index for evaluating women with anovulatory disease before, during, and after therapy.", "contents": "Human pituitary gonadotropin index. I. Standardized LRH test criteria for evaluation of functional amenorrhea. LRH test were carried out by giving 339 amenorrheic women and 74 normally menstruating volunteers an intravenous injection of 25 mug LRH (Hoe 471). Plasma LH and FSH were measured by RIA in two laboratories (Tuebingen and Ulm) using two standard reference preparations: LER 907 and 2nd IRP-HMG. The average conversion factors between the two standard preparations were calculated at 5.0 for LH and 25.0 for FSH. Furthermore, the estradiol-17beta levels were measured in 139 out of the 339 patients immediately before and 60 minutes after LRH injection. Taking the episodic and cyclic plasma gonadotropin fluctuations into consideration a shorthand system classifying the gonadotropin baseline (BI-BIV) and LH responses to 25 mug LRH (R0-R2) has been established and is referred to as Human Pituitary Gonadotropin Index (HPGI). It is possible to achieve reproducible gonadotropin results in two different laboratories using two different standard reference preparations. Two separate, randomly selected groups of amenorrheic women were found to have the same percent distribution of the HPGI. A correlation coefficient of r equal 0.67 between basal and LRH stimulated plasma LH levels does not sufficiently characterize the individual LH response behavior. A significant increase of plasma LH and FSH within the test period (60') reveals that the iv administration of 25 mug LRH represents an adequate dose for the LRH test in women. The HPGI which characterizes the functional state of gonadostat, may become a useful diagnostic index for evaluating women with anovulatory disease before, during, and after therapy."}
{"id": "PMID:1094033", "title": "Experimental gingivitis in young and elderly individuals.", "content": "The development of experimental gingivitis was studied in young elderly humans during a 21-d period of oral hygiene abstention. The state of the gingiva was assessed by the Gingival Index and by measurements of the amount of gingival exudate on filter paper strips placed at the entrance of the gingival sulcus of the lower lateral incisors and cuspids. Soft deposits were assessed by the Plaque Index and by differential counts of microorganisms in gram stained smears od ento-gingival plaque. At the end of the plaque growth period, the patients were given a thorough dental prophylaxis. Gingival condition and plaque were assessed at regular intervals during a subsequent period of controlled oral hygiene. The development of gingivitis during the oral hygiene abstention period was more rapid and more severe in old than in young individuals. Plaque accumulation was greater in the older persons. A definite difference in plaque consistency was alos observed. However, microscopic counts of various types of microorganisms did not reveal any differences throughout the period of plaque accumulation. When active oral hygiene was reinstituted, the state of the gingiva rapidly returned to pre-experimental levels in both groups. The findings of this study indicate that with age ther is an altered host response to the microorganisms of the plaque.", "contents": "Experimental gingivitis in young and elderly individuals. The development of experimental gingivitis was studied in young elderly humans during a 21-d period of oral hygiene abstention. The state of the gingiva was assessed by the Gingival Index and by measurements of the amount of gingival exudate on filter paper strips placed at the entrance of the gingival sulcus of the lower lateral incisors and cuspids. Soft deposits were assessed by the Plaque Index and by differential counts of microorganisms in gram stained smears od ento-gingival plaque. At the end of the plaque growth period, the patients were given a thorough dental prophylaxis. Gingival condition and plaque were assessed at regular intervals during a subsequent period of controlled oral hygiene. The development of gingivitis during the oral hygiene abstention period was more rapid and more severe in old than in young individuals. Plaque accumulation was greater in the older persons. A definite difference in plaque consistency was alos observed. However, microscopic counts of various types of microorganisms did not reveal any differences throughout the period of plaque accumulation. When active oral hygiene was reinstituted, the state of the gingiva rapidly returned to pre-experimental levels in both groups. The findings of this study indicate that with age ther is an altered host response to the microorganisms of the plaque."}
{"id": "PMID:1094034", "title": "Inhibitory effect of benzethonium and zinc chloride mouthrinses on human dental plaque and gingivitis.", "content": "In a double-blind study the plaque- and gingivitis-inhibiting capacities of three aqueous mouthrinse solutions of benzethonium chloride, zinc chloride, and benzethonium chloride combined with zinc chloride were compared to those of a placebo mouthrinse. Forth-three adults with clean teeth and healthy gingiva were randomly assigned to the treatment and control groups. All participants received an initial prophylaxis. Assessments of plaque and gingivitis were made before and after a 10-day test period. During this time the subjects suspended oral hygiene procedures and rinsed daily with 20 ml of their respective rinse solutions. According to the measurements made before and after the test period, the mouthrinse containing benzethonium chloride produced 42 to 42.9% less plaque than any of the other three formulations. The differences were statistically significant ( P smaller than 0.05). Gingivitis scores of the four groups did not differ significantly. These findings indicate that benzethonium chloride is a moderately effective antiplaque agent.", "contents": "Inhibitory effect of benzethonium and zinc chloride mouthrinses on human dental plaque and gingivitis. In a double-blind study the plaque- and gingivitis-inhibiting capacities of three aqueous mouthrinse solutions of benzethonium chloride, zinc chloride, and benzethonium chloride combined with zinc chloride were compared to those of a placebo mouthrinse. Forth-three adults with clean teeth and healthy gingiva were randomly assigned to the treatment and control groups. All participants received an initial prophylaxis. Assessments of plaque and gingivitis were made before and after a 10-day test period. During this time the subjects suspended oral hygiene procedures and rinsed daily with 20 ml of their respective rinse solutions. According to the measurements made before and after the test period, the mouthrinse containing benzethonium chloride produced 42 to 42.9% less plaque than any of the other three formulations. The differences were statistically significant ( P smaller than 0.05). Gingivitis scores of the four groups did not differ significantly. These findings indicate that benzethonium chloride is a moderately effective antiplaque agent."}
{"id": "PMID:1094035", "title": "Effect of professional tooth cleaning on healing after periodontal surgery.", "content": "The investigation was undertaken to find out whether favourable conditions for healing after periodontal surgery would develop in patients whose oral hygiene was professionally maintained at a high standard. The study was performed on 20 patients with advanced periodontal disease. Following an initial examination, comprising plaque index and gingival index scoring, measurement of pocket depths and loss of attachment, the patients were randomly distributed between a test and a control group. The patients first received professional cleaning of the teeth once every 2 weeks. The patients of the control group were recalled for scaling of the teeth once every 6 months. All patients were reexamined after 6, 12 and 24 months. It was found that the control patients were unable to maintain a high standard of oral hygiene with the result that the treatment of the periodontal disease failed. The patients of the test group maintained a high standard of oral hygiene, and the treatment of the periodontal disease was, therefore, successful.", "contents": "Effect of professional tooth cleaning on healing after periodontal surgery. The investigation was undertaken to find out whether favourable conditions for healing after periodontal surgery would develop in patients whose oral hygiene was professionally maintained at a high standard. The study was performed on 20 patients with advanced periodontal disease. Following an initial examination, comprising plaque index and gingival index scoring, measurement of pocket depths and loss of attachment, the patients were randomly distributed between a test and a control group. The patients first received professional cleaning of the teeth once every 2 weeks. The patients of the control group were recalled for scaling of the teeth once every 6 months. All patients were reexamined after 6, 12 and 24 months. It was found that the control patients were unable to maintain a high standard of oral hygiene with the result that the treatment of the periodontal disease failed. The patients of the test group maintained a high standard of oral hygiene, and the treatment of the periodontal disease was, therefore, successful."}
{"id": "PMID:1094036", "title": "A comparative clinical study of three periodontal surgical techniques.", "content": "A comparative 4-month trial was performed after initial preparation. The procedures used were curettage, replaced flap and apically repositioned flap procedures with osseous recontouring respectively in a split-mouth technique in 17 male and 23 female patients, 22 to 65 years old. Changes were assessed with plaque and gingival indices, and measurements of pocket depths, attachment levels and tissue contours. All procedures reduced pocket depths. Apically repositioned flaps were the most successful, reducing overall mean pockets from 3.5 to 1.9 mm. Changes in attachment levels post-operatively showed only a small degree of variation among the three surgical procedures, and would not affect the choice of the apically repositioned flap as the most effective method for pocket reduction. A marked improvement in tissue contour was obtained in those areas treated by apically repositioned flap procedures and osseous recontouring. Despite failure to improve tissue contour, replaced flap procedures showed an equal degree of success in maintaining plaque control when compared to apically repositioned flaps, as evidenced by static plaque indices. Replaced flap procedures were also the only group to show improved gingival health over the 4-month post-operative period with lower gingival indexes. It would seem that good healing, followed by a high standard of oral hygiene, may overcome the presence of gingival and marginal osseous deformities.", "contents": "A comparative clinical study of three periodontal surgical techniques. A comparative 4-month trial was performed after initial preparation. The procedures used were curettage, replaced flap and apically repositioned flap procedures with osseous recontouring respectively in a split-mouth technique in 17 male and 23 female patients, 22 to 65 years old. Changes were assessed with plaque and gingival indices, and measurements of pocket depths, attachment levels and tissue contours. All procedures reduced pocket depths. Apically repositioned flaps were the most successful, reducing overall mean pockets from 3.5 to 1.9 mm. Changes in attachment levels post-operatively showed only a small degree of variation among the three surgical procedures, and would not affect the choice of the apically repositioned flap as the most effective method for pocket reduction. A marked improvement in tissue contour was obtained in those areas treated by apically repositioned flap procedures and osseous recontouring. Despite failure to improve tissue contour, replaced flap procedures showed an equal degree of success in maintaining plaque control when compared to apically repositioned flaps, as evidenced by static plaque indices. Replaced flap procedures were also the only group to show improved gingival health over the 4-month post-operative period with lower gingival indexes. It would seem that good healing, followed by a high standard of oral hygiene, may overcome the presence of gingival and marginal osseous deformities."}
{"id": "PMID:1094039", "title": "Scanning electron microscopy of the subarachnoid space in the dog. III. Cranial levels.", "content": "Young dogs were anesthetized by intrathoracic injection of sodium pentobarbital and perfused with buffered aldehydes. Lining tissue samples from the cranial subarachnoid space were prepared for scanning electron microscopy (SEM) by postfixing in buffered OSO4. Samples were then dehydrated, dried in a Critical Point Drying System, and coated with carbon and palladium-gold. Specimens were viewed in a Cambridge S4 scanning electron microscope. After thorough scanning, selected samples were routinely prepared for transmission electron microscopy (TEM) and viewed in a Philips EM-200 transmission electron microscope. This study depicts the surface morphology of the meningeal linings of the cranial subarachnoid space. The cranial pia mater possesses natural gaps or fenestrations between cells. SEM reveals a more complex morphology of arachnoid trabeculae than previously interpreted from light and transmission electron microscopy. Many free cells are observed on the meningeal linings of the subarachnoid space. The present study establishes that these free cells are macrophages by means of definitive TEM correlates. Microvillous-like processes extending between macrophages and the pial surface are present. The frequency and the nature of these thin processes suggest the possibility of a plasmalemma-mediated system of communication.", "contents": "Scanning electron microscopy of the subarachnoid space in the dog. III. Cranial levels. Young dogs were anesthetized by intrathoracic injection of sodium pentobarbital and perfused with buffered aldehydes. Lining tissue samples from the cranial subarachnoid space were prepared for scanning electron microscopy (SEM) by postfixing in buffered OSO4. Samples were then dehydrated, dried in a Critical Point Drying System, and coated with carbon and palladium-gold. Specimens were viewed in a Cambridge S4 scanning electron microscope. After thorough scanning, selected samples were routinely prepared for transmission electron microscopy (TEM) and viewed in a Philips EM-200 transmission electron microscope. This study depicts the surface morphology of the meningeal linings of the cranial subarachnoid space. The cranial pia mater possesses natural gaps or fenestrations between cells. SEM reveals a more complex morphology of arachnoid trabeculae than previously interpreted from light and transmission electron microscopy. Many free cells are observed on the meningeal linings of the subarachnoid space. The present study establishes that these free cells are macrophages by means of definitive TEM correlates. Microvillous-like processes extending between macrophages and the pial surface are present. The frequency and the nature of these thin processes suggest the possibility of a plasmalemma-mediated system of communication."}
{"id": "PMID:1094038", "title": "Results of a one-year trial of tolmetin in patients with rheumatoid arthritis.", "content": "The safety and effectiveness of tolmetin was assessed in ten patients with active rheumatoid arthritis for 12 months. All patients met the requirements of the American Rheumatism Association's diagnostic criteria for definite rheumatoid arthritis. None of the patients had received gold, antimalarials, or glucocorticoids. All had been stabilized, more or less, with aspirin. Monthly measurements were made of grip strength, duration of morning stiffness, time to the onset of fatigue, Westergren sedimentation rates, the number of swollen joints, number of hot joints, the time to walk 50 feet, and the circumference of proximal interphalangeal (PIP) joints (measured in millimeters with a plastic-loop arthrocircameter). The initial dosage of tolmetin in all patients was 600 mg a day, given orally in three divided doses. Upon completion of the study, the average daily oral dosage was 1400 mg tolmetin. No other antiinflammatory drugs were allowed. The results indicate that tolmetin affords symptomatic relief of rheumatoid arthritis; its use results in global improvement in 80 per cent of the patients, and a net improvement in one or more of the parameters used to assess disease activity in 100 per cent of the patients; it seems to be sufficiently safe to administer to patients over a long period of time; there was no evidence of severe toxicity of any kind; gastrointestinal side effects were uncommon, and epigastric distress, in particular, was not observed.", "contents": "Results of a one-year trial of tolmetin in patients with rheumatoid arthritis. The safety and effectiveness of tolmetin was assessed in ten patients with active rheumatoid arthritis for 12 months. All patients met the requirements of the American Rheumatism Association's diagnostic criteria for definite rheumatoid arthritis. None of the patients had received gold, antimalarials, or glucocorticoids. All had been stabilized, more or less, with aspirin. Monthly measurements were made of grip strength, duration of morning stiffness, time to the onset of fatigue, Westergren sedimentation rates, the number of swollen joints, number of hot joints, the time to walk 50 feet, and the circumference of proximal interphalangeal (PIP) joints (measured in millimeters with a plastic-loop arthrocircameter). The initial dosage of tolmetin in all patients was 600 mg a day, given orally in three divided doses. Upon completion of the study, the average daily oral dosage was 1400 mg tolmetin. No other antiinflammatory drugs were allowed. The results indicate that tolmetin affords symptomatic relief of rheumatoid arthritis; its use results in global improvement in 80 per cent of the patients, and a net improvement in one or more of the parameters used to assess disease activity in 100 per cent of the patients; it seems to be sufficiently safe to administer to patients over a long period of time; there was no evidence of severe toxicity of any kind; gastrointestinal side effects were uncommon, and epigastric distress, in particular, was not observed."}
{"id": "PMID:1094050", "title": "Cements for permanent luting: a summarizing review.", "content": "Three cement systems are favored for permanent luting of cast restorations. These include zinc phosphate, reinforced zinc oxide-eugenol, and polycarboxylate cements. Although others have been used in the past and new luting media are anticipated for the future, the status of currently used cement for luting is based mainly on the results of laboratory research and on clinical experience. Zinc phosphate cement, with an impressive 100-year history, currently holds the advantage. Future comprehensive clinical studies, correlated with results of physical and biological testing, may eventually direct the clinican's preference to another, newer material. It is hoped that properties and strength values that are clinically significant also will be identified, so that laboratory tests can be more predictive of clinical success.", "contents": "Cements for permanent luting: a summarizing review. Three cement systems are favored for permanent luting of cast restorations. These include zinc phosphate, reinforced zinc oxide-eugenol, and polycarboxylate cements. Although others have been used in the past and new luting media are anticipated for the future, the status of currently used cement for luting is based mainly on the results of laboratory research and on clinical experience. Zinc phosphate cement, with an impressive 100-year history, currently holds the advantage. Future comprehensive clinical studies, correlated with results of physical and biological testing, may eventually direct the clinican's preference to another, newer material. It is hoped that properties and strength values that are clinically significant also will be identified, so that laboratory tests can be more predictive of clinical success."}
{"id": "PMID:1094054", "title": "Abnormalities in cell-mediated immunity in patients with Cryptococcus neoformans infection.", "content": "Cryptococcin, streptokinase-streptodornase (SK-SD), mumps, and purified protein derivative (PPD) were used for skin testing and, with whole killed Cryptococcus neoformans, were used in migration inhibition and lymphocyte transformation assays of control subjects and patients with past or present disseminated C. neoformans infection. Cryptococcin was found to differentiate control subjects grouped by known Cryptococcus exposure. Cryptococcin and C. neoformans were effective in stimulating leukocyte migration inhibition and lymphocyte transformation in the cryptococcin skin test-positive control subjects. Fifteen apparently normal patients who had been cured of cryptococcosis were found, as a group, to have impaired responsiveness to skin testing with cryptococcin and mumps, minimal leukocyte migration inhibition when stimulated with cryptococcin or C. neoformans, but normal group responses to cryptococcin in  Cryptococcus-induced lymphocyte transformation. Six patients with known predisposing conditions to disseminated infection (sarcoid, lymphoma, leukemia, steroid therapy) had markedly diminished responses to most skin tests and in vitro assays. It is suggested that the apparently normal individual who develops disseminated cryptococcal infection has subtle defects in cellular immunity that may have antedated and predisposed to infection.", "contents": "Abnormalities in cell-mediated immunity in patients with Cryptococcus neoformans infection. Cryptococcin, streptokinase-streptodornase (SK-SD), mumps, and purified protein derivative (PPD) were used for skin testing and, with whole killed Cryptococcus neoformans, were used in migration inhibition and lymphocyte transformation assays of control subjects and patients with past or present disseminated C. neoformans infection. Cryptococcin was found to differentiate control subjects grouped by known Cryptococcus exposure. Cryptococcin and C. neoformans were effective in stimulating leukocyte migration inhibition and lymphocyte transformation in the cryptococcin skin test-positive control subjects. Fifteen apparently normal patients who had been cured of cryptococcosis were found, as a group, to have impaired responsiveness to skin testing with cryptococcin and mumps, minimal leukocyte migration inhibition when stimulated with cryptococcin or C. neoformans, but normal group responses to cryptococcin in  Cryptococcus-induced lymphocyte transformation. Six patients with known predisposing conditions to disseminated infection (sarcoid, lymphoma, leukemia, steroid therapy) had markedly diminished responses to most skin tests and in vitro assays. It is suggested that the apparently normal individual who develops disseminated cryptococcal infection has subtle defects in cellular immunity that may have antedated and predisposed to infection."}
{"id": "PMID:1094055", "title": "Primary interaction between antibody and components of Alternaria. II. Antibodies in sera from normal, allergic, and immunoglobulin-deficient children.", "content": "Antibodies in sera from normal, allergic, and immunoglobulin-deficient children were studied for binding to radiolabeled components of Alternaria tenuis. Significant binding levels were found in 103 of 105 sera from normal children. The levels were age-dependent, rising from a low point in the 7- to 12-month age group to adult levels by the age of 8 years. Levels of binding to two antigens, a culture filtrate derivative (125I-CLF) and a mycelial derivative (125-I-HS), were similar. Sera from asthmatic children with strong immediate skin test reactions to Alternaria extracts bound significantly higher levels of 125-I-CLF than did sera from allergic children with negative skin tests or from control children. Binding levels in sera from children with hypogammaglobulinemia were significantly less than binding levels in sera from normal children in any age group. Sera from children with selective IgA  deficiency bound 125-I-CLF at normal levels. The almost universal occurrence of anti-Alternaria antibodies in children was partly explained by the finding of partial cross-reactivity and/or shared antigens among several fungal species, including A. tenuis, Stemphylium sp., Curvularia sp., and Aspergillus fumigatus. The biological significance of these antibodies is not clear, but the procedures described lend themselves to further investigations.", "contents": "Primary interaction between antibody and components of Alternaria. II. Antibodies in sera from normal, allergic, and immunoglobulin-deficient children. Antibodies in sera from normal, allergic, and immunoglobulin-deficient children were studied for binding to radiolabeled components of Alternaria tenuis. Significant binding levels were found in 103 of 105 sera from normal children. The levels were age-dependent, rising from a low point in the 7- to 12-month age group to adult levels by the age of 8 years. Levels of binding to two antigens, a culture filtrate derivative (125I-CLF) and a mycelial derivative (125-I-HS), were similar. Sera from asthmatic children with strong immediate skin test reactions to Alternaria extracts bound significantly higher levels of 125-I-CLF than did sera from allergic children with negative skin tests or from control children. Binding levels in sera from children with hypogammaglobulinemia were significantly less than binding levels in sera from normal children in any age group. Sera from children with selective IgA  deficiency bound 125-I-CLF at normal levels. The almost universal occurrence of anti-Alternaria antibodies in children was partly explained by the finding of partial cross-reactivity and/or shared antigens among several fungal species, including A. tenuis, Stemphylium sp., Curvularia sp., and Aspergillus fumigatus. The biological significance of these antibodies is not clear, but the procedures described lend themselves to further investigations."}
{"id": "PMID:1094071", "title": "Absence of hepatitis B antigens from feces and sewage as a result of enzymatic destruction.", "content": "Hepatitis B surface antigen (HBs Ag) was not detected in sewage by means of a sensitive affinity chromatography method combined with radioimmunoassay. The antigen was also absent from the feces and urine of 23 patients with HBs Ag antigenemia; this observation indicates that HBs Ag is rarely discharged into sewage. The absence of HBs Ag from feces is ascribed to antagonists of an enzymatic nature or to carboxypeptidase A, which destroys the antigen. Antagonists with similar effects were produced by three species of Pseudomonas but were not produced by various other bacteria. HBs Ag was also destroyed by two subtilisin enzymes. When hepatitis B sera were incubated with these enzymes or with the antagonists, small spherical particles, tubules, and the coats of Dane particles disappeared first, and Dane cores disappeared later. Although sewage or activated sludge did not affect the stability of HBs Ag, the results indicate that even Dane cores are not excreted in feces and that sewage plays a negligible role in the spread of HBs Ag, Dane cores, and viral hepatitis type B.", "contents": "Absence of hepatitis B antigens from feces and sewage as a result of enzymatic destruction. Hepatitis B surface antigen (HBs Ag) was not detected in sewage by means of a sensitive affinity chromatography method combined with radioimmunoassay. The antigen was also absent from the feces and urine of 23 patients with HBs Ag antigenemia; this observation indicates that HBs Ag is rarely discharged into sewage. The absence of HBs Ag from feces is ascribed to antagonists of an enzymatic nature or to carboxypeptidase A, which destroys the antigen. Antagonists with similar effects were produced by three species of Pseudomonas but were not produced by various other bacteria. HBs Ag was also destroyed by two subtilisin enzymes. When hepatitis B sera were incubated with these enzymes or with the antagonists, small spherical particles, tubules, and the coats of Dane particles disappeared first, and Dane cores disappeared later. Although sewage or activated sludge did not affect the stability of HBs Ag, the results indicate that even Dane cores are not excreted in feces and that sewage plays a negligible role in the spread of HBs Ag, Dane cores, and viral hepatitis type B."}
{"id": "PMID:1094072", "title": "Light and electron microscopic study of Chlamydia trachomatis infection of the uterine cervix.", "content": "Cervical biopsy specimens from two patients with infections caused by Chlamydia trachomatis (TRIC agent) were studied by means of light and electron microscopy. Intraepithelial vesicles containing C. trachomatis were present in the cervical mucosa. These intramucosal vesicles were readily recognized in paraffin sections by means of light microscopy. The developmental stages of C. trachomatis, which have previously been seen in tissue cell culture, chicken embryo, and conjunctival infections, were also apparent in cervical infection.", "contents": "Light and electron microscopic study of Chlamydia trachomatis infection of the uterine cervix. Cervical biopsy specimens from two patients with infections caused by Chlamydia trachomatis (TRIC agent) were studied by means of light and electron microscopy. Intraepithelial vesicles containing C. trachomatis were present in the cervical mucosa. These intramucosal vesicles were readily recognized in paraffin sections by means of light microscopy. The developmental stages of C. trachomatis, which have previously been seen in tissue cell culture, chicken embryo, and conjunctival infections, were also apparent in cervical infection."}
{"id": "PMID:1094073", "title": "Superiority of MacConkey's agar over salmonella-shigella agar for isolation of Shigella dysenteriae type 1.", "content": "The efficiency of MacConkey's agar in the isolation of various types of Shigella was compared with that of salmonella-shigella (SS) agar during an extensive 18-month outbreak of disease caused by Shigella. In all, 1,580 isolates of Shigella were obtained from 12,307 rectal swab and fecal samples of patients with diarrhea and their contacts by direct plating onto MacConkey's and SS agars. Shigella dysenteriae type 1 and Shigella flexneri constituted 55 percent and 33 percent of all isolates, respectively, with a smaller number of Shigella boydii and Shigella sonnei. MacConkey's agar was superior to SS agar in the detection of S. dysenteriae type 1; 83 percent of the isolates were detectable on MacConkey's agar, compared with 40 percent on SS agar. In contrast, 84 percent of S. flexneri isolates were detectable on SS agar, compared with 51 percent on MacConkey's agar alone. These findings confirm that, for the culture of fecal specimens thought to contain S. dysenteriae type 1, one of the media used should be noninhibitory. Shigella was isolated at a consistently and significantly higher rate from fecal samples than from rectal swabs.", "contents": "Superiority of MacConkey's agar over salmonella-shigella agar for isolation of Shigella dysenteriae type 1. The efficiency of MacConkey's agar in the isolation of various types of Shigella was compared with that of salmonella-shigella (SS) agar during an extensive 18-month outbreak of disease caused by Shigella. In all, 1,580 isolates of Shigella were obtained from 12,307 rectal swab and fecal samples of patients with diarrhea and their contacts by direct plating onto MacConkey's and SS agars. Shigella dysenteriae type 1 and Shigella flexneri constituted 55 percent and 33 percent of all isolates, respectively, with a smaller number of Shigella boydii and Shigella sonnei. MacConkey's agar was superior to SS agar in the detection of S. dysenteriae type 1; 83 percent of the isolates were detectable on MacConkey's agar, compared with 40 percent on SS agar. In contrast, 84 percent of S. flexneri isolates were detectable on SS agar, compared with 51 percent on MacConkey's agar alone. These findings confirm that, for the culture of fecal specimens thought to contain S. dysenteriae type 1, one of the media used should be noninhibitory. Shigella was isolated at a consistently and significantly higher rate from fecal samples than from rectal swabs."}
{"id": "PMID:1094074", "title": "Vitamin B6-dependent Streptococcus mitior (mitis) isolated from patients with systemic infections.", "content": "Strains of nutritional-variant viridans Streptococcus were isolated from two patients with bacterial endocarditis and from one patient with a pancreatic abscess. All three strains grew as satellite colonies of other bacteria but did not grow as pure cultures in media lacking sufficient thiol compounds. For one-half maximal growth these organisms required the addition of one of the active forms of the B6 vitamin, pyridoxal with HCl (0.16-1.45 mug/ml) or pyridoxamine dihydrochloride (0.67-2.0 mug/ml), or addition of l-cysteine (0.235-0.425 mg/ml) to routine bacteriological media. The active forms of vitamin B6 are essential coenzymes in the synthesis of l-cysteine and of other thiol compounds. Use of media supplemented with 0.001 percent pyridoxal with HCL led to identification of the strains as Streptococcus mitior (mitis). Incorporation of pyridoxal with HCl, pyridoxamine dihydrochloride, or l-cysteine into media is recommended for the isolation and identification of vitamin B6-dependent viridans streptococci.", "contents": "Vitamin B6-dependent Streptococcus mitior (mitis) isolated from patients with systemic infections. Strains of nutritional-variant viridans Streptococcus were isolated from two patients with bacterial endocarditis and from one patient with a pancreatic abscess. All three strains grew as satellite colonies of other bacteria but did not grow as pure cultures in media lacking sufficient thiol compounds. For one-half maximal growth these organisms required the addition of one of the active forms of the B6 vitamin, pyridoxal with HCl (0.16-1.45 mug/ml) or pyridoxamine dihydrochloride (0.67-2.0 mug/ml), or addition of l-cysteine (0.235-0.425 mg/ml) to routine bacteriological media. The active forms of vitamin B6 are essential coenzymes in the synthesis of l-cysteine and of other thiol compounds. Use of media supplemented with 0.001 percent pyridoxal with HCL led to identification of the strains as Streptococcus mitior (mitis). Incorporation of pyridoxal with HCl, pyridoxamine dihydrochloride, or l-cysteine into media is recommended for the isolation and identification of vitamin B6-dependent viridans streptococci."}
{"id": "PMID:1094075", "title": "Combined type-specific antisera in the identification of Mycoplasma hominis.", "content": "In the usual serologic test for Mycoplasma hominis, disks containing antibody to a prototype strain are applied to an agar plate that has been inoculated with the microorganism to be tested, and inhibition of growth is studied. The recent demonstration that M. hominis is not serologically homogeneous but consists of at least seven serotypes suggested the need for reexamination of the specificity of the disk test. The inhibition of growth of M. hominis on solid medium by disks containing seven antisera to the seven prototype strains was studied. Antisera to strains 1, 2, and 3 significantly inhibited all seven prototype strains, but the other four antisera did not inhibit the growth of all heterologous strains. The individual antisera 1, 2, and 3 did not inhibit all of a group of 19 recent genital isolates of M. hominis. However, when the three antisera were combined, they inhibited all 19 isolates as much as or more than any of the three alone. The growth of the prototype strain 5 (PG 21) and of some recent clinical isolates was not completely prevented by the homologous or reference antisera, but the growth of all was partially inhibited.", "contents": "Combined type-specific antisera in the identification of Mycoplasma hominis. In the usual serologic test for Mycoplasma hominis, disks containing antibody to a prototype strain are applied to an agar plate that has been inoculated with the microorganism to be tested, and inhibition of growth is studied. The recent demonstration that M. hominis is not serologically homogeneous but consists of at least seven serotypes suggested the need for reexamination of the specificity of the disk test. The inhibition of growth of M. hominis on solid medium by disks containing seven antisera to the seven prototype strains was studied. Antisera to strains 1, 2, and 3 significantly inhibited all seven prototype strains, but the other four antisera did not inhibit the growth of all heterologous strains. The individual antisera 1, 2, and 3 did not inhibit all of a group of 19 recent genital isolates of M. hominis. However, when the three antisera were combined, they inhibited all 19 isolates as much as or more than any of the three alone. The growth of the prototype strain 5 (PG 21) and of some recent clinical isolates was not completely prevented by the homologous or reference antisera, but the growth of all was partially inhibited."}
{"id": "PMID:1094077", "title": "Serum dopamine-beta-hydroxylase in familial dysautonomia.", "content": "The mean value of serum dopamine-beta-hydroxylase (D beta H) in patients with familial dysautonomia, 1 to 5 years of age, does not differ significantly from control children of the same age (24.0 plus or minus 21.06 S.D. as compared to 34.0 plus or minus 33.12). Among patients 6 years of age and over, the mean value was slightly but significantly lower than in control subjects (62.7 plus or minus 49.61, as compared to control values of 86.1 plus or minus 54.31 p less than 0.025). However, the determination of serum D beta H does not contribute to the diagnosis of familial dysautonomia because well over half the children have levels within 1 S.D. of the mean levels of the control subjects. There is no correlation with clinical symptomatology. The disease process may tend to depress the level of serum D beta H but the effect is neither consistent nor decisive.", "contents": "Serum dopamine-beta-hydroxylase in familial dysautonomia. The mean value of serum dopamine-beta-hydroxylase (D beta H) in patients with familial dysautonomia, 1 to 5 years of age, does not differ significantly from control children of the same age (24.0 plus or minus 21.06 S.D. as compared to 34.0 plus or minus 33.12). Among patients 6 years of age and over, the mean value was slightly but significantly lower than in control subjects (62.7 plus or minus 49.61, as compared to control values of 86.1 plus or minus 54.31 p less than 0.025). However, the determination of serum D beta H does not contribute to the diagnosis of familial dysautonomia because well over half the children have levels within 1 S.D. of the mean levels of the control subjects. There is no correlation with clinical symptomatology. The disease process may tend to depress the level of serum D beta H but the effect is neither consistent nor decisive."}
{"id": "PMID:1094078", "title": "Prevention by glucocorticoids of disseminated intravascular coagulation induced by endotoxin: mechanisms.", "content": "The therapeutic efficiency of two glucocorticoids (hydrocortisone and dexamethasone) on endotoxin-induced intravascular coagulation was investigated in the rat. Coagulation and platelet aggregation studies were performed and plaminogen was assayed. Our results indicate that pretreatment of the animals with large doses of these steroids within a few hours prior to endotoxin totally prevents the consumption in Hageman factor, measurable contact product activity, platelets, fibrinogen, plasminogen, and the loss in platelet aggregability and serotonin. In addition to this, the hypercoagulable state  consecutive to endotoxin, characterized here by shortenings in the partial thromboplastin and recalcification times and by an increase in the availability of platelet procoagulant activity, was also totally prevented by the steroid pretreatment. On the other hand, it is shown that these glucocorticoids do not interfere in the normal rat with platelet aggregation (tested with thrombin,adenosine diphosphate, and collagen), but with the availability of platelet procoagulant activity. This last phenomenon, in addition to that of an interference in vivo with the mechanism of activation of Hageman factor, are believed to be responsible for prevention by glucocorticoids of endotoxin-induced disseminated intravascular coagulation.", "contents": "Prevention by glucocorticoids of disseminated intravascular coagulation induced by endotoxin: mechanisms. The therapeutic efficiency of two glucocorticoids (hydrocortisone and dexamethasone) on endotoxin-induced intravascular coagulation was investigated in the rat. Coagulation and platelet aggregation studies were performed and plaminogen was assayed. Our results indicate that pretreatment of the animals with large doses of these steroids within a few hours prior to endotoxin totally prevents the consumption in Hageman factor, measurable contact product activity, platelets, fibrinogen, plasminogen, and the loss in platelet aggregability and serotonin. In addition to this, the hypercoagulable state  consecutive to endotoxin, characterized here by shortenings in the partial thromboplastin and recalcification times and by an increase in the availability of platelet procoagulant activity, was also totally prevented by the steroid pretreatment. On the other hand, it is shown that these glucocorticoids do not interfere in the normal rat with platelet aggregation (tested with thrombin,adenosine diphosphate, and collagen), but with the availability of platelet procoagulant activity. This last phenomenon, in addition to that of an interference in vivo with the mechanism of activation of Hageman factor, are believed to be responsible for prevention by glucocorticoids of endotoxin-induced disseminated intravascular coagulation."}
{"id": "PMID:1094079", "title": "Abnormal renal acidification in alcoholic liver disease.", "content": "After ingestion of 150 mEq. of calcium chloride (CaCl-2), urinary acidification was studied for 6 hours in 22 normokalemic patients with alcoholic liver disease (L) of varying severity, and in 7 control (C) subjects during 10 studies. The degree of the induced systemic acidosis was similar in all groups. Nine L patients were unable to normally lower urine pH below 5.25 (L-I) and these were compared with the 13 L patients achieving lower pH (L-II) and with control subjects. This defect was consistently reproduced. Titratable acid excretion was less in L-I than in the other groups. The percentage contribution of ammonium to maximal net acid excretion was significantly higher in L-I and L-II than in C. No L-I patient had spontaneous metabolic acidosis, nor was there evidence of encephalopathy or of proximal tubular dysfunction. Sodium excretion was significantly lower in L-I than in either L-II or C. Sodium sulfate and sodium phosphate infused after acid-loading rapidly reduced urine pH into the appropriately acidic range in L-I patients with alcoholic liver disease by means of a simple, safe, and short acid-loading test. Although the mechanism of this renal tubular acidfying defect remains unknown a low distal delivery of sodium by limiting the transtubular potential difference may have been partially responsible.", "contents": "Abnormal renal acidification in alcoholic liver disease. After ingestion of 150 mEq. of calcium chloride (CaCl-2), urinary acidification was studied for 6 hours in 22 normokalemic patients with alcoholic liver disease (L) of varying severity, and in 7 control (C) subjects during 10 studies. The degree of the induced systemic acidosis was similar in all groups. Nine L patients were unable to normally lower urine pH below 5.25 (L-I) and these were compared with the 13 L patients achieving lower pH (L-II) and with control subjects. This defect was consistently reproduced. Titratable acid excretion was less in L-I than in the other groups. The percentage contribution of ammonium to maximal net acid excretion was significantly higher in L-I and L-II than in C. No L-I patient had spontaneous metabolic acidosis, nor was there evidence of encephalopathy or of proximal tubular dysfunction. Sodium excretion was significantly lower in L-I than in either L-II or C. Sodium sulfate and sodium phosphate infused after acid-loading rapidly reduced urine pH into the appropriately acidic range in L-I patients with alcoholic liver disease by means of a simple, safe, and short acid-loading test. Although the mechanism of this renal tubular acidfying defect remains unknown a low distal delivery of sodium by limiting the transtubular potential difference may have been partially responsible."}
{"id": "PMID:1094090", "title": "Mutations in Salmonella typhimurium conferring resistance to Felix O phage without loss of smooth character.", "content": "Several mutants obtained from smooth Salmonella typhimurium strains by selection for resistance to Felix O (FO) phage [whose receptor site includes the N-acetylglucosamine branch of the lipopolysaccharide (LPS) core] were smooth in cultural properties, antigenic character and phage sensitivity pattern (except for their FO resistance). However, the affected genes of several such 'FOR' (FO-resistant) mutants were shown by transduction of map in the short cysE-pyrE segment, which includes nearly all known rfa genes responsible for synthesis of LPS core. All of seven FOR mutants differed from their parents, and resembled rfa mutants with defects in the deeper part of the LPS core, by increased sensitivity to various antibiotics. One FOR mutant was non-virulent (LD50 greater than 10-7, compared with smaller than 100 for its parent); LT7 derivatives given this FOR gene by co-transduction with cysE+ were likewise non-virulent. It is inferred that FOR mutations affect the assembly of the inner part of the LPS core, perhaps causing incomplete blocks in glycosyl transferase reactions.", "contents": "Mutations in Salmonella typhimurium conferring resistance to Felix O phage without loss of smooth character. Several mutants obtained from smooth Salmonella typhimurium strains by selection for resistance to Felix O (FO) phage [whose receptor site includes the N-acetylglucosamine branch of the lipopolysaccharide (LPS) core] were smooth in cultural properties, antigenic character and phage sensitivity pattern (except for their FO resistance). However, the affected genes of several such 'FOR' (FO-resistant) mutants were shown by transduction of map in the short cysE-pyrE segment, which includes nearly all known rfa genes responsible for synthesis of LPS core. All of seven FOR mutants differed from their parents, and resembled rfa mutants with defects in the deeper part of the LPS core, by increased sensitivity to various antibiotics. One FOR mutant was non-virulent (LD50 greater than 10-7, compared with smaller than 100 for its parent); LT7 derivatives given this FOR gene by co-transduction with cysE+ were likewise non-virulent. It is inferred that FOR mutations affect the assembly of the inner part of the LPS core, perhaps causing incomplete blocks in glycosyl transferase reactions."}
{"id": "PMID:1094091", "title": "Transmissible substrate-utilizing ability in enterobacteria.", "content": "Three of 152 strains of Escherichia coli transmitted their ability to utilize sucrose (Sac+) to other strains by conjugation. The transfer factor of one of them and of a Sac+ Salmonella thompson strain was thermosensitive. The raffinose-utilizing ability of 27 of 163 E. coli strains was also transmissible. Transmissible raffinose-utilizing ability was a feature of porcine enterpathogenic strains possessing the K88 antigen. The determinants controlling raffinose utilization (Raf) and K88 antigen production were commonly transmitted together from these strains; so also was the determinant controlling enterotoxin production, but to a lesser extent. It was not possible to transfer lactose-utilizing ability from 320 strains of E. coli, salicin-utilizing ability from 12 strains of E. coli or dulcitol-utilizing ability from 99 strains of E. coli and 88 strains of salmonellae. Sucrose- and raffinose-utilizing ability were transmitted separately to several Salmonella sp., including Salm. typhi, to Shigella flexneri and Sh. sonnei and to a variety of strains of E. coli. A strain of Salm. typhimurium in which Sac had been established and a strain of E. coli in which Raf had been established survived less well in the alimentary tract of chickens than their Sac minus or Raf minus parent strains.", "contents": "Transmissible substrate-utilizing ability in enterobacteria. Three of 152 strains of Escherichia coli transmitted their ability to utilize sucrose (Sac+) to other strains by conjugation. The transfer factor of one of them and of a Sac+ Salmonella thompson strain was thermosensitive. The raffinose-utilizing ability of 27 of 163 E. coli strains was also transmissible. Transmissible raffinose-utilizing ability was a feature of porcine enterpathogenic strains possessing the K88 antigen. The determinants controlling raffinose utilization (Raf) and K88 antigen production were commonly transmitted together from these strains; so also was the determinant controlling enterotoxin production, but to a lesser extent. It was not possible to transfer lactose-utilizing ability from 320 strains of E. coli, salicin-utilizing ability from 12 strains of E. coli or dulcitol-utilizing ability from 99 strains of E. coli and 88 strains of salmonellae. Sucrose- and raffinose-utilizing ability were transmitted separately to several Salmonella sp., including Salm. typhi, to Shigella flexneri and Sh. sonnei and to a variety of strains of E. coli. A strain of Salm. typhimurium in which Sac had been established and a strain of E. coli in which Raf had been established survived less well in the alimentary tract of chickens than their Sac minus or Raf minus parent strains."}
{"id": "PMID:1094092", "title": "The effect of culture age, chloramphenicol and B6 inhibitors on intra- and extracellular keto and amino acids of Escherichia coli B.", "content": "Keto acids and free amino acids were assayed in the cells and the medium of Escherichia coli B growing in the presence of chloramphenicol, cycloserines, aminooxyacetate, and limiting nitrogen source. Under these growth-limiting conditions the cells accumulated ketoglutarate and 'ketovaline' but no other keto acids. In all experiments only ketoglutarate, pyruvate, and 'ketovaline' were found in the medium. Amino acids are released into the medium in the early phases of growth and the composition of the extracellular amino acids is similar to that of the amino acid pool. The concentrations of free amino acids were 10-3-10-4 times higher in the cell than in the medium. The internal pool composition is fixed under all growth-limiting conditions. In the presence of the drugs the cells release amino acids into the medium.", "contents": "The effect of culture age, chloramphenicol and B6 inhibitors on intra- and extracellular keto and amino acids of Escherichia coli B. Keto acids and free amino acids were assayed in the cells and the medium of Escherichia coli B growing in the presence of chloramphenicol, cycloserines, aminooxyacetate, and limiting nitrogen source. Under these growth-limiting conditions the cells accumulated ketoglutarate and 'ketovaline' but no other keto acids. In all experiments only ketoglutarate, pyruvate, and 'ketovaline' were found in the medium. Amino acids are released into the medium in the early phases of growth and the composition of the extracellular amino acids is similar to that of the amino acid pool. The concentrations of free amino acids were 10-3-10-4 times higher in the cell than in the medium. The internal pool composition is fixed under all growth-limiting conditions. In the presence of the drugs the cells release amino acids into the medium."}
{"id": "PMID:1094096", "title": "Factors affecting the changes in amphotericin sensitivity of Candida albicans during growth.", "content": "The sensitivity of Candida albicans, grown in batch culture at 37 degrees C, to amphotericin methyl ester (AME), judged by the concentration of AME required to induce a standard rate of leakage of K+ from suspensions of organisms, decreased with the time of growth. Organisms in exponential growth were sensitive to 0-I to 0-2 mug AME/ml while organisms in the stationary phase were resistant to 4 to 60 mug AME/ml, depending on the initial concentration of glucose in the medium and the length of time for which incubation had been continued. When the initial concentration of glucose was low (0-I%, w/v), the AME resistance rose during the early stationary phase and then, after 40 h incubation at 37 degrees C, decrease again. Sphaeroplasts were prepared from organisms at different phases of growth and did not show these changes in AME sensitivity, but remained highly sensitive for growth up to 40 h. Sphaeroplasts were prepared by treating suspensions of organisms with mercaptoethanol and then digesting with Streptomyces enzyme preparation. Addition of the material extracted by the digestion to suspensions of exponential-phase organisms or sphaeroplasts increased their AME resistance. Fractionation of the digest showed that the antagonistic material was contained in the neutral lipid fraction. Pure lipids fell into the following order of decreasing antagonism to AME when added together with the antibiotic to suspensions of exponential-phase organisms: sterol esters (ergosterol esters greater than cholesterol esters; unsaturated fatty acid esters greater than saturated fatty acid esters), sterols, triglycerides, unsaturated fatty acids, saturated fatty acids. The amount of antagonistic material released from stationary organisms was not markedly greater than that from exponential-phase organisms and analysis of the lipid content of wall preparations showed that the content of total lipid, neutral lipid and triglyceride of 40 h organisms was not more than 75, 25 and 30%, respectively, greater than that of exponential-phase organisms. The AME resistance of stationary-phase organisms decreased rapidly if suspensions were incubated with glucose or mercaptoethanol. The decrease in the presence of glucose was prevented by metabolic inhibitors, especially SH binding agents. Treatment of organisms with either iodoacetamide or N-ethylmaleimide gave a rapid increase in AME resistance, amounting in some cases to 5- to 15-fold. The effect of iodoacetamide decreased as the organisms passed into the stationary phase and their intrinsic resistance increased. Evidence is presented which suggests that the degree of reduction of SH groups in the cell surface is an important factor in determining AME resistance.", "contents": "Factors affecting the changes in amphotericin sensitivity of Candida albicans during growth. The sensitivity of Candida albicans, grown in batch culture at 37 degrees C, to amphotericin methyl ester (AME), judged by the concentration of AME required to induce a standard rate of leakage of K+ from suspensions of organisms, decreased with the time of growth. Organisms in exponential growth were sensitive to 0-I to 0-2 mug AME/ml while organisms in the stationary phase were resistant to 4 to 60 mug AME/ml, depending on the initial concentration of glucose in the medium and the length of time for which incubation had been continued. When the initial concentration of glucose was low (0-I%, w/v), the AME resistance rose during the early stationary phase and then, after 40 h incubation at 37 degrees C, decrease again. Sphaeroplasts were prepared from organisms at different phases of growth and did not show these changes in AME sensitivity, but remained highly sensitive for growth up to 40 h. Sphaeroplasts were prepared by treating suspensions of organisms with mercaptoethanol and then digesting with Streptomyces enzyme preparation. Addition of the material extracted by the digestion to suspensions of exponential-phase organisms or sphaeroplasts increased their AME resistance. Fractionation of the digest showed that the antagonistic material was contained in the neutral lipid fraction. Pure lipids fell into the following order of decreasing antagonism to AME when added together with the antibiotic to suspensions of exponential-phase organisms: sterol esters (ergosterol esters greater than cholesterol esters; unsaturated fatty acid esters greater than saturated fatty acid esters), sterols, triglycerides, unsaturated fatty acids, saturated fatty acids. The amount of antagonistic material released from stationary organisms was not markedly greater than that from exponential-phase organisms and analysis of the lipid content of wall preparations showed that the content of total lipid, neutral lipid and triglyceride of 40 h organisms was not more than 75, 25 and 30%, respectively, greater than that of exponential-phase organisms. The AME resistance of stationary-phase organisms decreased rapidly if suspensions were incubated with glucose or mercaptoethanol. The decrease in the presence of glucose was prevented by metabolic inhibitors, especially SH binding agents. Treatment of organisms with either iodoacetamide or N-ethylmaleimide gave a rapid increase in AME resistance, amounting in some cases to 5- to 15-fold. The effect of iodoacetamide decreased as the organisms passed into the stationary phase and their intrinsic resistance increased. Evidence is presented which suggests that the degree of reduction of SH groups in the cell surface is an important factor in determining AME resistance."}
{"id": "PMID:1094097", "title": "Reversible inactivation of the isocitrate dehydrogenase of Escherichia coli ML308 during growth on acetate.", "content": "During aerobic growth of Escherichia coli ML308 on acetate as sole carbon source, the apparent synthesis of isocitrate dehydrogenase was repressed relative to cultures on other carbon sources, such as glucose, which do not employ the glyoxylate bypass as an anaplerotic sequence. When cells were removed from an acetate medium, or when compounds were added which made the operation of the glyoxylate bypass unnecessary, the activity of isocitrate dehydrogenase rapidly increased 3- to 4-fold but fell again on restoration to an acetate medium. Changes in activity were rapid and, furthermore, could be demonstrated in the absence of protein synthesis. It is thus improbable that the mechanism involved degradation or de novo synthesis of the enzyme protein. Oxaloacetate and glyoxylate showed concerted inhibition of isocitrate dehydrogenase which could be relieved by dialysis. Because extracts of low enzyme activity, derived from acetate-metabolizing cells, could not be stimulated by dialysis or by addition of a wide range of metabolites, it is unlikely that low molecular weight, freely dissociable effectors were responsible for stimulation or inhibition of activity. Control of isocitrate dehydrogenase permitted the efficient utilization of acetate as sole source of carbon and energy but perserved the capacity of the cell to respond rapidly to an improvement in nutritional conditions. A limited survey showed that the mechanism is common but not universal among strains of E. coli and occurs in at least one strain each of Klebsiella aerogenes, Salmonella typhimurium and Serratia marcescens.", "contents": "Reversible inactivation of the isocitrate dehydrogenase of Escherichia coli ML308 during growth on acetate. During aerobic growth of Escherichia coli ML308 on acetate as sole carbon source, the apparent synthesis of isocitrate dehydrogenase was repressed relative to cultures on other carbon sources, such as glucose, which do not employ the glyoxylate bypass as an anaplerotic sequence. When cells were removed from an acetate medium, or when compounds were added which made the operation of the glyoxylate bypass unnecessary, the activity of isocitrate dehydrogenase rapidly increased 3- to 4-fold but fell again on restoration to an acetate medium. Changes in activity were rapid and, furthermore, could be demonstrated in the absence of protein synthesis. It is thus improbable that the mechanism involved degradation or de novo synthesis of the enzyme protein. Oxaloacetate and glyoxylate showed concerted inhibition of isocitrate dehydrogenase which could be relieved by dialysis. Because extracts of low enzyme activity, derived from acetate-metabolizing cells, could not be stimulated by dialysis or by addition of a wide range of metabolites, it is unlikely that low molecular weight, freely dissociable effectors were responsible for stimulation or inhibition of activity. Control of isocitrate dehydrogenase permitted the efficient utilization of acetate as sole source of carbon and energy but perserved the capacity of the cell to respond rapidly to an improvement in nutritional conditions. A limited survey showed that the mechanism is common but not universal among strains of E. coli and occurs in at least one strain each of Klebsiella aerogenes, Salmonella typhimurium and Serratia marcescens."}
{"id": "PMID:1094101", "title": "Antidermatophytic activity of 2-thiotetrahydro-1,3,5-thiadiazines and isothiocyanates.", "content": "Benzyl, furfuryl, tetrahydronaphthyl, alpha-picolyl, and alpha-homopicolyl derivatives of 2-thiotetrahydro-1,3,5-thiadiazines were studied and found to be more active than beta-picolyl, and pyridyl analogs on dermatophytes, including the more resistant Microsporum canis.", "contents": "Antidermatophytic activity of 2-thiotetrahydro-1,3,5-thiadiazines and isothiocyanates. Benzyl, furfuryl, tetrahydronaphthyl, alpha-picolyl, and alpha-homopicolyl derivatives of 2-thiotetrahydro-1,3,5-thiadiazines were studied and found to be more active than beta-picolyl, and pyridyl analogs on dermatophytes, including the more resistant Microsporum canis."}
{"id": "PMID:1094105", "title": "Antimicrobial evaluation of diastereoisomeric 2-dimethylaminomethyl-6-phenylcyclohexanols and related esters.", "content": "2-Dimethylaminomethyl-6-phenylcyclohexanone was reduced to give the isomeric 2-dimethylaminomethyl-6-phenylcyclohexanols, which were then converted to a number of novel esters. These derivatives were screened against a wide range of microorganisms. The alcohol possessing an axial hydroxy group had high activity against Candida albicans, in contradistinction to the isomer having an equatorial hydroxy function, which was approximately 30 times less active. The esters from the isomeric alcohols demonstrated a low level of antimicrobial activity with the exception of the 10-undecenoyl ester of 2-dimethylaminomethyl-6-phenylcyclohexanol (possessing the equatorial hydroxy group), which showed significant activity against three pathogenic fungi.", "contents": "Antimicrobial evaluation of diastereoisomeric 2-dimethylaminomethyl-6-phenylcyclohexanols and related esters. 2-Dimethylaminomethyl-6-phenylcyclohexanone was reduced to give the isomeric 2-dimethylaminomethyl-6-phenylcyclohexanols, which were then converted to a number of novel esters. These derivatives were screened against a wide range of microorganisms. The alcohol possessing an axial hydroxy group had high activity against Candida albicans, in contradistinction to the isomer having an equatorial hydroxy function, which was approximately 30 times less active. The esters from the isomeric alcohols demonstrated a low level of antimicrobial activity with the exception of the 10-undecenoyl ester of 2-dimethylaminomethyl-6-phenylcyclohexanol (possessing the equatorial hydroxy group), which showed significant activity against three pathogenic fungi."}
{"id": "PMID:1094106", "title": "Effect of intracerebroventricular bradykinin and related peptides on rabbit operant behavior.", "content": "The dose-effect relationships of intraventricularly injected bradykinin, Gly-Arg-Met-Lys-bradykinin (GAML-bradykinin), synthetic substance P and angiotensin II on lever-lifting behavior of rabbits in a variable-interval (VI) 72-second schedule of sweetened water presentation were determined. All peptides used caused dose-dependent decreases in overall rates of VI responding during the experimental session in the following order of potency: angiotensin II greater than bradykinin = substance P greater than GAML-bradykinin. The angiotensin II dose-effect curve was less steep than those of the other peptides. The administration of nearly equimolar doses of the bradykinin potentiating peptides, BPP5a and BPP9a, slightly decreased overall VI response rates and caused a 10- to 20-fold potentiation of the rate-decreasing effect of bradykinin on VI responding. Both angiotensin II and bradykinin caused pauses in responding of dose-dependent duration at the beginning of the experimental session that were followed by normal VI responding. The effect of GAML-bradykinin on VI performance was similar to that of bradykinin and angiotensin II but had a delay of onset of 3 to 6 minutes. In contrast, substance P caused actual decreases in response output and pauses of variable duration interspersed between periods of regular VI responding. At the doses used, both bradykinin-potentiating peptides caused uniform decreases in VI responding throughout the experimental session. Gross behavioral changes caused by the peptides were also observed. After the intraventricular injection of bradykinin or GAML-bradykinin, rabbits showed decreased motility, ptosis, miosis and lowered ears; after angiotensin II, animals remained motionless but with wide open eyes, fully raised ears and no miosis. In turn, substance P caused restlessness and increased locomotion. These results together with reported evidence on other powerful central actions of bradykinin, angiotensin and substance P and on the existence of components of their releasing and destroying enzymatic systems in the brain suggest that linear peptides may play a role in the functioning of the central nervous system.", "contents": "Effect of intracerebroventricular bradykinin and related peptides on rabbit operant behavior. The dose-effect relationships of intraventricularly injected bradykinin, Gly-Arg-Met-Lys-bradykinin (GAML-bradykinin), synthetic substance P and angiotensin II on lever-lifting behavior of rabbits in a variable-interval (VI) 72-second schedule of sweetened water presentation were determined. All peptides used caused dose-dependent decreases in overall rates of VI responding during the experimental session in the following order of potency: angiotensin II greater than bradykinin = substance P greater than GAML-bradykinin. The angiotensin II dose-effect curve was less steep than those of the other peptides. The administration of nearly equimolar doses of the bradykinin potentiating peptides, BPP5a and BPP9a, slightly decreased overall VI response rates and caused a 10- to 20-fold potentiation of the rate-decreasing effect of bradykinin on VI responding. Both angiotensin II and bradykinin caused pauses in responding of dose-dependent duration at the beginning of the experimental session that were followed by normal VI responding. The effect of GAML-bradykinin on VI performance was similar to that of bradykinin and angiotensin II but had a delay of onset of 3 to 6 minutes. In contrast, substance P caused actual decreases in response output and pauses of variable duration interspersed between periods of regular VI responding. At the doses used, both bradykinin-potentiating peptides caused uniform decreases in VI responding throughout the experimental session. Gross behavioral changes caused by the peptides were also observed. After the intraventricular injection of bradykinin or GAML-bradykinin, rabbits showed decreased motility, ptosis, miosis and lowered ears; after angiotensin II, animals remained motionless but with wide open eyes, fully raised ears and no miosis. In turn, substance P caused restlessness and increased locomotion. These results together with reported evidence on other powerful central actions of bradykinin, angiotensin and substance P and on the existence of components of their releasing and destroying enzymatic systems in the brain suggest that linear peptides may play a role in the functioning of the central nervous system."}
{"id": "PMID:1094107", "title": "An enzymatic derivative double isotope assay for measuring tissue levels of taurine.", "content": "The development of a new assay for taurine is described. The procedure utilizes the formation of taurocholic acid by rat liver microsomes and is dependent upon the dilution of the specific activity of radioactive taurine by the amount of taurine present in perchloric acid homogenates of tissues. Known amounts of taurine (21/2-300 nmol) are added to incubation vessels to establish a standard curve so that unknown quantities of taurine present in tissue homogenates can be calculated by a graphical comparison. The possible interference by other compounds present in tissue extracts and their removal by passage through columns containing both cation and anion exchange resins is discussed. Conditions are established for the quantitative recovery of taurine after passage through the exchange resins. Measurement of the taurine levels of several rat tissues is presented along with duplicate assays performed on the amino acid analyzer for comparison purposes.", "contents": "An enzymatic derivative double isotope assay for measuring tissue levels of taurine. The development of a new assay for taurine is described. The procedure utilizes the formation of taurocholic acid by rat liver microsomes and is dependent upon the dilution of the specific activity of radioactive taurine by the amount of taurine present in perchloric acid homogenates of tissues. Known amounts of taurine (21/2-300 nmol) are added to incubation vessels to establish a standard curve so that unknown quantities of taurine present in tissue homogenates can be calculated by a graphical comparison. The possible interference by other compounds present in tissue extracts and their removal by passage through columns containing both cation and anion exchange resins is discussed. Conditions are established for the quantitative recovery of taurine after passage through the exchange resins. Measurement of the taurine levels of several rat tissues is presented along with duplicate assays performed on the amino acid analyzer for comparison purposes."}
{"id": "PMID:1094108", "title": "Regional sensitivity of the rat brain to the inhibitory effects of morphine on wet shake behavior.", "content": "The aim of this investigation was to determine the brain regions which were most sensitive to the inhibitory effects of morphine on the shaking response of pentobarbital-anesthetized rats to ice water. The median inhibitory dose (ID50) of morphine sulfate administered intraventricularly was found to be 0.35 mug/rat. When morphine was bilaterally injected into different regions of the brain, the ID50 values ranged from 0.04 to 17.9 mug/rat. The lowest ID50 values (0.04-0.20 mug) were found in the periaqueductal gray, the medial preoptic area and the locus ceruleus. The ID50 values ranged from 0.65 to 1.6 mug for areas around the nucleus accumbens, the fasciculus retroflexus, the medical thalamus and the septal area; from 5.6 to 7.3 mug for various hypothalamic nuclei; and from 11.0 to 17.9 mug for the basal ganglia, reticular formation substantia nigra and the reticular nucleus of the thalamus. The brain areas with the lowest ID50 values are known to have thermoregulatory functions. The similarity of the shaking response to shivering is discussed. It is concluded that the central inhibitory effects of morphine on shaking are subserved by discrete neuroanatomical substrates located in medial subcortical structures.", "contents": "Regional sensitivity of the rat brain to the inhibitory effects of morphine on wet shake behavior. The aim of this investigation was to determine the brain regions which were most sensitive to the inhibitory effects of morphine on the shaking response of pentobarbital-anesthetized rats to ice water. The median inhibitory dose (ID50) of morphine sulfate administered intraventricularly was found to be 0.35 mug/rat. When morphine was bilaterally injected into different regions of the brain, the ID50 values ranged from 0.04 to 17.9 mug/rat. The lowest ID50 values (0.04-0.20 mug) were found in the periaqueductal gray, the medial preoptic area and the locus ceruleus. The ID50 values ranged from 0.65 to 1.6 mug for areas around the nucleus accumbens, the fasciculus retroflexus, the medical thalamus and the septal area; from 5.6 to 7.3 mug for various hypothalamic nuclei; and from 11.0 to 17.9 mug for the basal ganglia, reticular formation substantia nigra and the reticular nucleus of the thalamus. The brain areas with the lowest ID50 values are known to have thermoregulatory functions. The similarity of the shaking response to shivering is discussed. It is concluded that the central inhibitory effects of morphine on shaking are subserved by discrete neuroanatomical substrates located in medial subcortical structures."}
{"id": "PMID:1094114", "title": "Synthesis and evaluation of 6-arylactamido-2,4-diaminoquinazolines and related compounds as folic acid antagonists.", "content": "A series of 2,4-diaminoquinazolines bearing an aryl function attached to the 6 position through an acetamido or related linkage was synthesized. Each compound was evaluated as an inhibitor of rat liver dihydrofolate reductase as well as for suppressive antimalarial effects against Plasmodium berghei in mice. Significant in vivo activity was found to reside primarily with 5-chloro-6-arylacetamido derivatives. Most of these compounds were also tested for prophylactic activity against sporozoite-induced Plasmodium gallinaceum in chicks. Thirteen compounds, each of which possesses a 5-Cl or 5-CH3 group, displayed curative activity in this test system. Since several of these showed markedly greater potency against the avian infection, selective inhibitory action upon preerythrocytic forms of the malaria parasite is thus implied.", "contents": "Synthesis and evaluation of 6-arylactamido-2,4-diaminoquinazolines and related compounds as folic acid antagonists. A series of 2,4-diaminoquinazolines bearing an aryl function attached to the 6 position through an acetamido or related linkage was synthesized. Each compound was evaluated as an inhibitor of rat liver dihydrofolate reductase as well as for suppressive antimalarial effects against Plasmodium berghei in mice. Significant in vivo activity was found to reside primarily with 5-chloro-6-arylacetamido derivatives. Most of these compounds were also tested for prophylactic activity against sporozoite-induced Plasmodium gallinaceum in chicks. Thirteen compounds, each of which possesses a 5-Cl or 5-CH3 group, displayed curative activity in this test system. Since several of these showed markedly greater potency against the avian infection, selective inhibitory action upon preerythrocytic forms of the malaria parasite is thus implied."}
{"id": "PMID:1094115", "title": "Synthesis and biological activity of LH-RH analogs modified at the carboxyl terminus.", "content": "Des(Pro9,Gly10)-LH-RH ethylamide, des(Pro9,Gly10)-LH-RH butylamide, desGly10-LH-RH 2-aminoethylamide, and desGly10-LH-RH hydrazide were synthesized by a solid-phase method involving cleavage of protected peptide intermediates from their resin support by reaction with ethylamine, butylamine, ethylenediamine, and hydrazine, respectively. In the assay utilizing steroid pretreated, ovariectomized rats, the peptides were found to have the following LH-releasing activities when compared with natural LH-RH: ethylamide, 0.2%; butylamide, 0,1%; 2-aminoethylamide, 2.4%; hydrazide, 12%. DesGly10-LH-RH hydrazide was used as a precursor in the synthesis of desGly10-LH-RH allylamide and desGly10-LH-RH propargylamide by conversion to the azide and reaction with allylamine and propargylamine, respectively. LH and FSH levels were measured over a 4-hr period after subcutaneous injection of these two peptides into immature male rats in order to detect any prolongation of activity. The allylamide analog was quite active, releasing 1.7 times more LH and 1.3 times more FSH than the same dose of LH-RH. The propargylamide analog was considerably less active, exhibiting 50% LH-releasing activity and 64% FSH-releasing activity. Neither peptide appeared to be longer acting than LH-RH.", "contents": "Synthesis and biological activity of LH-RH analogs modified at the carboxyl terminus. Des(Pro9,Gly10)-LH-RH ethylamide, des(Pro9,Gly10)-LH-RH butylamide, desGly10-LH-RH 2-aminoethylamide, and desGly10-LH-RH hydrazide were synthesized by a solid-phase method involving cleavage of protected peptide intermediates from their resin support by reaction with ethylamine, butylamine, ethylenediamine, and hydrazine, respectively. In the assay utilizing steroid pretreated, ovariectomized rats, the peptides were found to have the following LH-releasing activities when compared with natural LH-RH: ethylamide, 0.2%; butylamide, 0,1%; 2-aminoethylamide, 2.4%; hydrazide, 12%. DesGly10-LH-RH hydrazide was used as a precursor in the synthesis of desGly10-LH-RH allylamide and desGly10-LH-RH propargylamide by conversion to the azide and reaction with allylamine and propargylamine, respectively. LH and FSH levels were measured over a 4-hr period after subcutaneous injection of these two peptides into immature male rats in order to detect any prolongation of activity. The allylamide analog was quite active, releasing 1.7 times more LH and 1.3 times more FSH than the same dose of LH-RH. The propargylamide analog was considerably less active, exhibiting 50% LH-releasing activity and 64% FSH-releasing activity. Neither peptide appeared to be longer acting than LH-RH."}
{"id": "PMID:1094116", "title": "Synthesis and antitrichomonal activity of certain pyrazolo (1,5-a) pyrimidines.", "content": "Several bridgehead nitrogen heterocycles were synthesized to be screened as antimicrobial agents, modeled after nalidixic acid. The activity of these new compounds, all derivatives of 3-nitro-4,6-disubstituted pyrazolo (1,5-a)pyrimidin-7-ones (3,7,8, and 9), however, was found to be highly specific for Trichomonas foetus and completely lacking in activity against bacteria, fungi, and parasites other than Trichomonas. Of the nine componds synthesized, including the intermediate 4,6-disubstituted pyrazolo (1,5-a) pyrimidin-7-ones (2-6) and the 6-substituted or unsubstituted pyrazolo (1,5-a) pyrimidin-7-ones (1 and 4), only 6-carbethoxy-4-ethyl-3-nitropyrazolo(1,5-a)pyrimidin-7-one (7) was found to be a potent antitrichomal agent, being comparable or perhaps better than metronidazole. From a tentative structure-activity relationship study, it was apparent that the combination of the 3-mitro, 4-ethyl, and 6-carbethoxy groups imparted specific activity, wheras other substitutions imparted little or no antitrichomonal activity.", "contents": "Synthesis and antitrichomonal activity of certain pyrazolo (1,5-a) pyrimidines. Several bridgehead nitrogen heterocycles were synthesized to be screened as antimicrobial agents, modeled after nalidixic acid. The activity of these new compounds, all derivatives of 3-nitro-4,6-disubstituted pyrazolo (1,5-a)pyrimidin-7-ones (3,7,8, and 9), however, was found to be highly specific for Trichomonas foetus and completely lacking in activity against bacteria, fungi, and parasites other than Trichomonas. Of the nine componds synthesized, including the intermediate 4,6-disubstituted pyrazolo (1,5-a) pyrimidin-7-ones (2-6) and the 6-substituted or unsubstituted pyrazolo (1,5-a) pyrimidin-7-ones (1 and 4), only 6-carbethoxy-4-ethyl-3-nitropyrazolo(1,5-a)pyrimidin-7-one (7) was found to be a potent antitrichomal agent, being comparable or perhaps better than metronidazole. From a tentative structure-activity relationship study, it was apparent that the combination of the 3-mitro, 4-ethyl, and 6-carbethoxy groups imparted specific activity, wheras other substitutions imparted little or no antitrichomonal activity."}
{"id": "PMID:1094117", "title": "Preparation of a new immunosuppressant, 4-5-bis(aminomethyl)acridine.", "content": "4,5-Bis(aminomethyl)acridine, useful as an immunosuppressant, was prepared in 31% overall yield by the reaction of acridine with N-(hydroxymethyl)phthalimide and subsequent decomposition with excess 6 N HCl. The drug was found to produce a suppression of the humoral antibody response comparable to several known immunosuppressive agents.", "contents": "Preparation of a new immunosuppressant, 4-5-bis(aminomethyl)acridine. 4,5-Bis(aminomethyl)acridine, useful as an immunosuppressant, was prepared in 31% overall yield by the reaction of acridine with N-(hydroxymethyl)phthalimide and subsequent decomposition with excess 6 N HCl. The drug was found to produce a suppression of the humoral antibody response comparable to several known immunosuppressive agents."}
{"id": "PMID:1094118", "title": "Analysis of expert diagnosis of a computer simulation of congenital heart disease.", "content": "Analysis of data from five expert cardiologists indicates substantial agreement on the first step to be taken in response to an emergency case of computer-simulated congenital heart disease. There was little or no agreement among these same subjects on the specific steps undertaken following their initial response. The problem was solved in 11, 10, 8, and 6 steps by four of the cardiologists. The problem-solving sequence employed by one expert resulted in the patient's death. It is concluded that while the behavior of experts in the hospital and clinic is the primary means of evaluating successful student performance, computer simulations of patient cases offer the opportunity to use expert data in the calibration of student error.", "contents": "Analysis of expert diagnosis of a computer simulation of congenital heart disease. Analysis of data from five expert cardiologists indicates substantial agreement on the first step to be taken in response to an emergency case of computer-simulated congenital heart disease. There was little or no agreement among these same subjects on the specific steps undertaken following their initial response. The problem was solved in 11, 10, 8, and 6 steps by four of the cardiologists. The problem-solving sequence employed by one expert resulted in the patient's death. It is concluded that while the behavior of experts in the hospital and clinic is the primary means of evaluating successful student performance, computer simulations of patient cases offer the opportunity to use expert data in the calibration of student error."}
{"id": "PMID:1094122", "title": "Stabilization and clarification of sections mounted on formvar-coated slot grids by deliberate irradiation in the electron beam.", "content": "Epon-Araldite sections supported on Formvar-coated slot grids can be stabilized by exposing the area of interest in the column of the electron microscope to irradiation of 0-5C/cm-2. With this procedure one can obtain electron micrographs of high quality, without drift or distortion, from any part of the open slot area. After treatment the support film is not only stable and transparent but robust enough to permit repeated removal and replacement of the specimen in the microscope column. We illustrate the method with a series of electron micrographs with initial magnifications ranging from x300 to x500,000 to show the quality of image that can be achieved. The effect of the irradiation on tissue architecture is checked by resectioning the irradiated sections. We recommend this method of local irradiation of sections for investigations using low or intermediate magnifications for the wide field it permits, for the durability it confers on the specimen and for its convenience.", "contents": "Stabilization and clarification of sections mounted on formvar-coated slot grids by deliberate irradiation in the electron beam. Epon-Araldite sections supported on Formvar-coated slot grids can be stabilized by exposing the area of interest in the column of the electron microscope to irradiation of 0-5C/cm-2. With this procedure one can obtain electron micrographs of high quality, without drift or distortion, from any part of the open slot area. After treatment the support film is not only stable and transparent but robust enough to permit repeated removal and replacement of the specimen in the microscope column. We illustrate the method with a series of electron micrographs with initial magnifications ranging from x300 to x500,000 to show the quality of image that can be achieved. The effect of the irradiation on tissue architecture is checked by resectioning the irradiated sections. We recommend this method of local irradiation of sections for investigations using low or intermediate magnifications for the wide field it permits, for the durability it confers on the specimen and for its convenience."}
{"id": "PMID:1094123", "title": "High resolution scanning electron microscopy at the subcellular level.", "content": "Recently developed scanning electron microscopes provide sufficient resolution to allow useful observation of subcellular biological objects. Preparation methods for such objects need not be limited to the traditional coating and mounting procedures. Many methods developed for transmission electron microscopy are immediately adaptable to scanning electron microscopy. We show that a number of techniques are available to the microscopist which yield adequate contrast and high resolution. As examples we show skeletal muscle myofibrils dispersed to reveal thick filaments, uncoated on a thin carbon film; a tropomyosin tactoid, negatively stained with uranyl acetate; oncornavirus, conventionally coated; and T4 bacteriophage on an aluminium substrate.", "contents": "High resolution scanning electron microscopy at the subcellular level. Recently developed scanning electron microscopes provide sufficient resolution to allow useful observation of subcellular biological objects. Preparation methods for such objects need not be limited to the traditional coating and mounting procedures. Many methods developed for transmission electron microscopy are immediately adaptable to scanning electron microscopy. We show that a number of techniques are available to the microscopist which yield adequate contrast and high resolution. As examples we show skeletal muscle myofibrils dispersed to reveal thick filaments, uncoated on a thin carbon film; a tropomyosin tactoid, negatively stained with uranyl acetate; oncornavirus, conventionally coated; and T4 bacteriophage on an aluminium substrate."}
{"id": "PMID:1094124", "title": "A technique for processing mucous coated marine invertebrate spermatozoa for scanning electron microscopy.", "content": "A technique for preparing heavily mucous coated marine invertebrate spermatozoa for scanning electron microscopy (SEM) is described. This technique involves washing in 1500 NF units/ml hyaluronidase in millipored sea water to remove mucus, followed by fixation in glutaraldehyde and osmium tetroxide. Following primary fixation, spermatozoa are enclosed in Nuclepore membrane bags positioned within Teflon specimen capsules allowing them to be processed and critical point dried without excessive mechanical damage or loss.", "contents": "A technique for processing mucous coated marine invertebrate spermatozoa for scanning electron microscopy. A technique for preparing heavily mucous coated marine invertebrate spermatozoa for scanning electron microscopy (SEM) is described. This technique involves washing in 1500 NF units/ml hyaluronidase in millipored sea water to remove mucus, followed by fixation in glutaraldehyde and osmium tetroxide. Following primary fixation, spermatozoa are enclosed in Nuclepore membrane bags positioned within Teflon specimen capsules allowing them to be processed and critical point dried without excessive mechanical damage or loss."}
{"id": "PMID:1094125", "title": "Comparative survival of lethally irradiated inbred male mice inoculated with marrow from virgin or multiparous female donors.", "content": "The response of marrow from virgin female donors to the antigens controlled by the Y chromosome of syngeneic male recipients varied with different strains of mice and was independent of their H-2 genotype. Marrow from syngeneic multiparous donors might produce a greater response to male antigens than that observed with marrow from virgin females. The sensitization of the mother to her male progeny indicated that these antigens were expressed before parturition. The reduced responsiveness of mothers to some alloantigens and the accompanying increased responsiveness to male antigens suggested a difference in the time during embryonic development when these antigens were expressed.", "contents": "Comparative survival of lethally irradiated inbred male mice inoculated with marrow from virgin or multiparous female donors. The response of marrow from virgin female donors to the antigens controlled by the Y chromosome of syngeneic male recipients varied with different strains of mice and was independent of their H-2 genotype. Marrow from syngeneic multiparous donors might produce a greater response to male antigens than that observed with marrow from virgin females. The sensitization of the mother to her male progeny indicated that these antigens were expressed before parturition. The reduced responsiveness of mothers to some alloantigens and the accompanying increased responsiveness to male antigens suggested a difference in the time during embryonic development when these antigens were expressed."}
{"id": "PMID:1094126", "title": "Response of congenitally athymic (nude) mice to infection with Mycobacterium bovis (strain BCG).", "content": "Mice homozygous for the mutation \"nude\" (nu/nu) are athymic and lack thymus-dependent lymphocytes. Heterozygote (nu/+) controls, which are phenotypically and immunologically normal, and (nu/nu) mice were infected with 1.0 times 10-6 colony-forming units of Phipps strain BCG. In the spleens of nu/+ mice, there was a progressive increase in number of BCG up to the second week, followed by a gradual decline. However, in the nu/nu mice, BCG growth was gradual and continuous until termination of the experiment at 5 weeks. In the lung, significant differences were not noted until after the second week, at which time the nude mice showed a rapid increase (of more than 2 log10) in the number of BCG. However, the number of BCG was not significantly greater in the livers of either group. Changes in the normal histology of the lung included a massive influx of monocytes during the first 2 weeks which peaked at day 21. In the lungs of the nu/+ mice by day 28, there was considerable granuloma formation consisting of monocytes and small lymphocytes. However, in the lungs of nu/nu animals, the granulomas were made up primarily of monocytes with a lack of small lymphocytes. Acid-fast stains confirmed the presence of large numbers of organisms in the macrophages of nu/nu mice, with gradual destruction of these phagocytic cells.", "contents": "Response of congenitally athymic (nude) mice to infection with Mycobacterium bovis (strain BCG). Mice homozygous for the mutation \"nude\" (nu/nu) are athymic and lack thymus-dependent lymphocytes. Heterozygote (nu/+) controls, which are phenotypically and immunologically normal, and (nu/nu) mice were infected with 1.0 times 10-6 colony-forming units of Phipps strain BCG. In the spleens of nu/+ mice, there was a progressive increase in number of BCG up to the second week, followed by a gradual decline. However, in the nu/nu mice, BCG growth was gradual and continuous until termination of the experiment at 5 weeks. In the lung, significant differences were not noted until after the second week, at which time the nude mice showed a rapid increase (of more than 2 log10) in the number of BCG. However, the number of BCG was not significantly greater in the livers of either group. Changes in the normal histology of the lung included a massive influx of monocytes during the first 2 weeks which peaked at day 21. In the lungs of the nu/+ mice by day 28, there was considerable granuloma formation consisting of monocytes and small lymphocytes. However, in the lungs of nu/nu animals, the granulomas were made up primarily of monocytes with a lack of small lymphocytes. Acid-fast stains confirmed the presence of large numbers of organisms in the macrophages of nu/nu mice, with gradual destruction of these phagocytic cells."}
{"id": "PMID:1094132", "title": "Transport in bacteriophage P22-infected Salmonella typhimurium.", "content": "There was rapid efflux of L-leucine, L-phenylalanine, and alpha-methyl-D-glucoside after infection of Salmonella typhimurium with the clear plaque mutant C1 of phage P22. The efflux was similar to that observed with cyanide or arsenate treatment except that there was partial recovery in the case of phage infection and almost complete recovery under the condition of lysogeny. There was no efflux after infection with the temperature-sensitive mutant ts16C1 at nonpermissive temperature. Superinfection of superinfection exclusion negative lysogen (sie A minus sie B minus) with C1 led to efflux, whereas the efflux was much less on superinfection of sie A+ Sie B+ lysogen. These results indicate that an effective injection process is enough to cause depression in the cellular transport processes.", "contents": "Transport in bacteriophage P22-infected Salmonella typhimurium. There was rapid efflux of L-leucine, L-phenylalanine, and alpha-methyl-D-glucoside after infection of Salmonella typhimurium with the clear plaque mutant C1 of phage P22. The efflux was similar to that observed with cyanide or arsenate treatment except that there was partial recovery in the case of phage infection and almost complete recovery under the condition of lysogeny. There was no efflux after infection with the temperature-sensitive mutant ts16C1 at nonpermissive temperature. Superinfection of superinfection exclusion negative lysogen (sie A minus sie B minus) with C1 led to efflux, whereas the efflux was much less on superinfection of sie A+ Sie B+ lysogen. These results indicate that an effective injection process is enough to cause depression in the cellular transport processes."}
{"id": "PMID:1094133", "title": "Effect of DNA delay mutations of bacteriophage T4 on genetic recombination.", "content": "Studies have been made of the effect of the DNA delay mutations of bacteriophage T4 on growth and genetic recombination in a number of Escherichia coli hosts. DNA delay mutations in genes 39, 52, 58 (61), and 60 result in abnormally high recombination frequencies. These high recombination frequencies are discussed in the context of other observations.", "contents": "Effect of DNA delay mutations of bacteriophage T4 on genetic recombination. Studies have been made of the effect of the DNA delay mutations of bacteriophage T4 on growth and genetic recombination in a number of Escherichia coli hosts. DNA delay mutations in genes 39, 52, 58 (61), and 60 result in abnormally high recombination frequencies. These high recombination frequencies are discussed in the context of other observations."}
{"id": "PMID:1094134", "title": "Presence of active polyribosomes in bacterial cells infected with T4 bacteriophage ghosts.", "content": "Host protein synthesis of Escherichia coli stops abruptly after T4 bacteriophage ghost infection. When infection was carried out in the presence of 10 mM Mg2plus, infected cells still have active polyribosomes despite the complete stoppage of protein synthesis. On the other hand, when T4 ghost infection was carried out in the presence of 1 mM Mg2plus, no polyribosomes were observed and most of the ribosomes were 30S and 50S subunit particles. Subunits obtained from extracts of ghost-infected cells at 1 mM M'G2++ concentration could not be converted to polyribosomes, even when Mg2plus concentration was adjusted to 10 mM after ghost infection. There was very little difference in amino acid incorporation activities between polyribosomes from ghost-infected and uninfected cells. In addition, the activity of 70S ribosomes isolated from uninfected cells was identical to that from cells infected with ghosts at 10 mM Mg2plus.", "contents": "Presence of active polyribosomes in bacterial cells infected with T4 bacteriophage ghosts. Host protein synthesis of Escherichia coli stops abruptly after T4 bacteriophage ghost infection. When infection was carried out in the presence of 10 mM Mg2plus, infected cells still have active polyribosomes despite the complete stoppage of protein synthesis. On the other hand, when T4 ghost infection was carried out in the presence of 1 mM Mg2plus, no polyribosomes were observed and most of the ribosomes were 30S and 50S subunit particles. Subunits obtained from extracts of ghost-infected cells at 1 mM M'G2++ concentration could not be converted to polyribosomes, even when Mg2plus concentration was adjusted to 10 mM after ghost infection. There was very little difference in amino acid incorporation activities between polyribosomes from ghost-infected and uninfected cells. In addition, the activity of 70S ribosomes isolated from uninfected cells was identical to that from cells infected with ghosts at 10 mM Mg2plus."}
{"id": "PMID:1094135", "title": "Resolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF.", "content": "The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the parent phage. In particular, resolution of the DNA strands of the specialized transducing phage lambda-h80C1-857dargF is described, and evidence is presented which indicates that argF is transcribed from the r strand.", "contents": "Resolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF. The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the parent phage. In particular, resolution of the DNA strands of the specialized transducing phage lambda-h80C1-857dargF is described, and evidence is presented which indicates that argF is transcribed from the r strand."}
{"id": "PMID:1094142", "title": "Ruptured arteriosclerotic aneurysms of the abdominal aorta. Reducing mortality.", "content": "Mortality in 100 patients undergoing resection of ruptured atherosclerotic aneurysms of the abdominal aorta was 58%. In addition to advanced age and concurrent cardiac disease, lethal factors included preoperative hypotension, cardiac arrest, anuria, and free intra-abdominal rupture. Failure to establish the correct diagnosis until the onset of these four signs contributed to the high mortality.", "contents": "Ruptured arteriosclerotic aneurysms of the abdominal aorta. Reducing mortality. Mortality in 100 patients undergoing resection of ruptured atherosclerotic aneurysms of the abdominal aorta was 58%. In addition to advanced age and concurrent cardiac disease, lethal factors included preoperative hypotension, cardiac arrest, anuria, and free intra-abdominal rupture. Failure to establish the correct diagnosis until the onset of these four signs contributed to the high mortality."}
{"id": "PMID:1094162", "title": "Interstitial immune complex nephritis in patients with systemic lupus erythematosus.", "content": "Interstitial immune complex nephritis in patients with systemic lupus erythematosus (SLE). Renal tissues from 45 patients with SLE nephritis, 34 patients with idiopathic membranous nephropathy (IMN) and 77 patients with minimal glomerular disease (MGD) were studied by light, immunofluorescence and electron microscopy. Interstitial nephritis characterized by focal or diffuse infiltration of inflammatory cells, tubular damage and interstitial fibrosis was observed in 66% of SLE patients. Fluorescein-conjugated antibodies to immunoglobulins or complement or both were bound to peritubular capillaries, interstitium and tubular basement membranes (TBM) in 53% of patients with a granular pattern corresponding to opaque deposits seen by light or electron microscopy or both. Antibodies reactive with thymidine or cytosine or both were bound to interstitial structures in 19% of patients tested and showed the same granular distribution. Interstitial cellular infiltration was rare and deposits of immunoglobulins and complement were rare or absent in IMN and MGD, whereas deposits of DNA products were never observed. The findings are consistent with the interpretation that in patients with SLE nephritis immune deposits, presumably containing DNA-anti-DNA complexes, localize in peritublular capillaries, TBM and interstitum, thereby producing an inflammatory reaction which contributes to development and evolution of renal diseases.", "contents": "Interstitial immune complex nephritis in patients with systemic lupus erythematosus. Interstitial immune complex nephritis in patients with systemic lupus erythematosus (SLE). Renal tissues from 45 patients with SLE nephritis, 34 patients with idiopathic membranous nephropathy (IMN) and 77 patients with minimal glomerular disease (MGD) were studied by light, immunofluorescence and electron microscopy. Interstitial nephritis characterized by focal or diffuse infiltration of inflammatory cells, tubular damage and interstitial fibrosis was observed in 66% of SLE patients. Fluorescein-conjugated antibodies to immunoglobulins or complement or both were bound to peritubular capillaries, interstitium and tubular basement membranes (TBM) in 53% of patients with a granular pattern corresponding to opaque deposits seen by light or electron microscopy or both. Antibodies reactive with thymidine or cytosine or both were bound to interstitial structures in 19% of patients tested and showed the same granular distribution. Interstitial cellular infiltration was rare and deposits of immunoglobulins and complement were rare or absent in IMN and MGD, whereas deposits of DNA products were never observed. The findings are consistent with the interpretation that in patients with SLE nephritis immune deposits, presumably containing DNA-anti-DNA complexes, localize in peritublular capillaries, TBM and interstitum, thereby producing an inflammatory reaction which contributes to development and evolution of renal diseases."}
{"id": "PMID:1094185", "title": "Relationship between pancreatic lesions and serum glucose values in Mystromys albicaudatus.", "content": "Serum glucose levels and pancreatic islet cell changes were studied in combination in 127 Mystromys albicaudatus in an effort to develop better criteria for defining diabetes mellitus in thiss rodent. The study supports a previous report in which 170 mg/100 ml was selected as indicative of hyperglycemia based on statistical analysis of serum glucose distributions. The presence of exceptions to predicted correlations between islet cell changes and serum glucose levels was identified for further study.", "contents": "Relationship between pancreatic lesions and serum glucose values in Mystromys albicaudatus. Serum glucose levels and pancreatic islet cell changes were studied in combination in 127 Mystromys albicaudatus in an effort to develop better criteria for defining diabetes mellitus in thiss rodent. The study supports a previous report in which 170 mg/100 ml was selected as indicative of hyperglycemia based on statistical analysis of serum glucose distributions. The presence of exceptions to predicted correlations between islet cell changes and serum glucose levels was identified for further study."}
{"id": "PMID:1094186", "title": "A technic for the collection of peritoneal cells from laboratory animals.", "content": "A simple method was devised for the collection of peritoneal cells from rats, Chinese hamsters, and mice using a special glass pipette. Cell collection was performed by lavage on the intact unanesthetized animal within 30-60 sec, and it yielded 5-20 million cells per rat and 0.3-2.5 million cells per mouse or Chinese hamster. The procedure can be repeated on different occasions in the same animal without apparent adverse effects.", "contents": "A technic for the collection of peritoneal cells from laboratory animals. A simple method was devised for the collection of peritoneal cells from rats, Chinese hamsters, and mice using a special glass pipette. Cell collection was performed by lavage on the intact unanesthetized animal within 30-60 sec, and it yielded 5-20 million cells per rat and 0.3-2.5 million cells per mouse or Chinese hamster. The procedure can be repeated on different occasions in the same animal without apparent adverse effects."}
{"id": "PMID:1094205", "title": "Lung water accumulation with acute hemodilution in dogs.", "content": "Extravascular lung water content was determined in vivo, gravimetrically, and histologically in severely hemodiluted (Ringer's lactate), supine, spontaneously breathing, halothane-anesthetized dogs. One group of dogs was studied immediately after undergoing hemodilution to a hematocrit value of less than 10 per cent; a second group was studied after hemodilution and 1 hour of circulatory maintenance with Ringer's lactate; and a third group was evaluated after hemodilution, maintenance for one hour with Ringer's lactate, and reconstitution of oncotic pressure with 75 Gm human salt-poor albumin. Lung water content was increased significantly from a normal of 3.88 Gm water per gram of dry weight to 4.70 Gm per gram of dry weight by hemodilution; it was increased further to 5.71 tgm per gram of dry weight during 1 hour of maintenance with Ringer's lactate. Reconstitution of oncotic pressure decreased the water content significantly to 4.96 and decreased the units demonstrating perivascular-peribronchial cuffing from 81 to 23 per cent. Double indication-dilution measurement of PEVW failed to reliably reflect changes in lung water. Arterial PO2 with the animals breathing 100 per cent oxygen was unchanged in all of the groups. We made the following conclusions: (1) lung water accumulation occurs during severe hemodilution and serum protein depletion; (2) this may be partially reversed by restoration of oncotic pressure; (3) double indicator-dilution PEVW measurements do not reliably reflect changes in extravascular lung water of less than 47 per cent; and (4) interstitial edema of this magnitude does not interfere with blood-gas exchange.", "contents": "Lung water accumulation with acute hemodilution in dogs. Extravascular lung water content was determined in vivo, gravimetrically, and histologically in severely hemodiluted (Ringer's lactate), supine, spontaneously breathing, halothane-anesthetized dogs. One group of dogs was studied immediately after undergoing hemodilution to a hematocrit value of less than 10 per cent; a second group was studied after hemodilution and 1 hour of circulatory maintenance with Ringer's lactate; and a third group was evaluated after hemodilution, maintenance for one hour with Ringer's lactate, and reconstitution of oncotic pressure with 75 Gm human salt-poor albumin. Lung water content was increased significantly from a normal of 3.88 Gm water per gram of dry weight to 4.70 Gm per gram of dry weight by hemodilution; it was increased further to 5.71 tgm per gram of dry weight during 1 hour of maintenance with Ringer's lactate. Reconstitution of oncotic pressure decreased the water content significantly to 4.96 and decreased the units demonstrating perivascular-peribronchial cuffing from 81 to 23 per cent. Double indication-dilution measurement of PEVW failed to reliably reflect changes in lung water. Arterial PO2 with the animals breathing 100 per cent oxygen was unchanged in all of the groups. We made the following conclusions: (1) lung water accumulation occurs during severe hemodilution and serum protein depletion; (2) this may be partially reversed by restoration of oncotic pressure; (3) double indicator-dilution PEVW measurements do not reliably reflect changes in extravascular lung water of less than 47 per cent; and (4) interstitial edema of this magnitude does not interfere with blood-gas exchange."}
{"id": "PMID:1094214", "title": "Antimicrobial agents from higher plants.  The quaternary alkaloids of Ptelea trifoliata.", "content": "Quaternary alkaloid extracts of Ptelea trifoliata showed potentially interesting antimicrobial activity.  Chromatographic examination showed the presence of six components.  Of these, choline and O-4-methyl ptelefolinium were known compounds.  Rational structures are proposed for the new alkaloids, O-4-methylhydroxyluninium, neohydroxylunine and pteleatinium salts.  Pteleatinium salt is responsible for the antimicrobial activity of the extracts of the plant.", "contents": "Antimicrobial agents from higher plants.  The quaternary alkaloids of Ptelea trifoliata. Quaternary alkaloid extracts of Ptelea trifoliata showed potentially interesting antimicrobial activity.  Chromatographic examination showed the presence of six components.  Of these, choline and O-4-methyl ptelefolinium were known compounds.  Rational structures are proposed for the new alkaloids, O-4-methylhydroxyluninium, neohydroxylunine and pteleatinium salts.  Pteleatinium salt is responsible for the antimicrobial activity of the extracts of the plant."}
{"id": "PMID:1094215", "title": "Quantitation of amanitins in Amanita verna with calf thymus RNA polymerase B.", "content": "A procedure utilizing the specific inhibition of calf thymus DNA-directed RNA polymerase B has been applied to the quantitation of amanitins.  This procedure has permitted the accurate quantitation of alpha-amanitin in amounts as low as 0.05 nanogram, a sensitivity 2000-fold greater than chemical detection methods used following tlc.  Analysis of extracts of specimens of Amanita verna identified by morphological criteria has demonstrated that while toxin concentration is variable, some specimens are practically devoid of amanitins and may represent a variety of A. verna or a distinct species.", "contents": "Quantitation of amanitins in Amanita verna with calf thymus RNA polymerase B. A procedure utilizing the specific inhibition of calf thymus DNA-directed RNA polymerase B has been applied to the quantitation of amanitins.  This procedure has permitted the accurate quantitation of alpha-amanitin in amounts as low as 0.05 nanogram, a sensitivity 2000-fold greater than chemical detection methods used following tlc.  Analysis of extracts of specimens of Amanita verna identified by morphological criteria has demonstrated that while toxin concentration is variable, some specimens are practically devoid of amanitins and may represent a variety of A. verna or a distinct species."}
{"id": "PMID:1094253", "title": "[New sequential therapy in the premenopause (author's transl)].", "content": "A report about the treatment of 85 women in the premenopause during 387 cycles is given. The treatment consisted in sequential therapy in form of conjugated Estrogens (Presomen) and a new synthetic Gestagen, which was given in three different modifications: from the fifth to the 24-th day of the cycle 1,25 mg Presomen a day, additionally on the last five, seven or ten days, during each period 6 respectively 5 mg Gestagen a day. It proved that both, the disfunctional haemorrhages as well as the climacteric syndrome, were favourably influenced. The mode 10+10, that is, 10 days Estrogens alone + 10 days combined with ;estagen, proved best. A sufficiently secure contraception is not guaranteed. This can be recognized by the occurence of biphasic basal temperature curves.", "contents": "[New sequential therapy in the premenopause (author's transl)]. A report about the treatment of 85 women in the premenopause during 387 cycles is given. The treatment consisted in sequential therapy in form of conjugated Estrogens (Presomen) and a new synthetic Gestagen, which was given in three different modifications: from the fifth to the 24-th day of the cycle 1,25 mg Presomen a day, additionally on the last five, seven or ten days, during each period 6 respectively 5 mg Gestagen a day. It proved that both, the disfunctional haemorrhages as well as the climacteric syndrome, were favourably influenced. The mode 10+10, that is, 10 days Estrogens alone + 10 days combined with ;estagen, proved best. A sufficiently secure contraception is not guaranteed. This can be recognized by the occurence of biphasic basal temperature curves."}
{"id": "PMID:1094255", "title": "[Indication and technic of thrombectomy in the therapy of thrombosis].", "content": "The problems of appropriate treatment of thrombosis are as follow: 1. the prevention of a lung-embolus, 2. the rapid rehabilitation of the venous circulation, 3. the avoidance of a post-thrombotic syndrom. The best ways of meeting these demands are through fibrinolysis as well as by operative treatment (thrombectomy). According to the author's findings, 58 cases of thrombectomy of acute, deep thrombosis of the veins have been reported with regards to the indication and technic of this method of treatment of thrombosis. Indications for thrombectomy are phlegmasia coerulea dolens and the occurrence of a mobile thrombosis as well as an unsuccessful treatment with fibrinolysis.", "contents": "[Indication and technic of thrombectomy in the therapy of thrombosis]. The problems of appropriate treatment of thrombosis are as follow: 1. the prevention of a lung-embolus, 2. the rapid rehabilitation of the venous circulation, 3. the avoidance of a post-thrombotic syndrom. The best ways of meeting these demands are through fibrinolysis as well as by operative treatment (thrombectomy). According to the author's findings, 58 cases of thrombectomy of acute, deep thrombosis of the veins have been reported with regards to the indication and technic of this method of treatment of thrombosis. Indications for thrombectomy are phlegmasia coerulea dolens and the occurrence of a mobile thrombosis as well as an unsuccessful treatment with fibrinolysis."}
{"id": "PMID:1094256", "title": "[A case of sarcoidosis with simultaneous primary hyperparathyroidism, coincedence or consequnce?].", "content": "We have reported of a case of generalised sarcoidosis with primary hyperparathyroidism.  A pathogenetic relation between sarcoidosis associated with hypercalcemia and the development of parathyroid adenoma will be discussed and a causal connection will be proposed.  According to our hypothesis every primary hyperparathyroidism could have developed from regulatory hyperfunction.  This is illustrated by sarcoidosis with hypercalcemia and hypercalcuria.  In this case a disturbance in vitamin D dependent calcium metabolism induces hyperplasia of the parathyroid which later leads to the development of a parathyroid adenoma.  In addition a review of literature describing similar cases is given.", "contents": "[A case of sarcoidosis with simultaneous primary hyperparathyroidism, coincedence or consequnce?]. We have reported of a case of generalised sarcoidosis with primary hyperparathyroidism.  A pathogenetic relation between sarcoidosis associated with hypercalcemia and the development of parathyroid adenoma will be discussed and a causal connection will be proposed.  According to our hypothesis every primary hyperparathyroidism could have developed from regulatory hyperfunction.  This is illustrated by sarcoidosis with hypercalcemia and hypercalcuria.  In this case a disturbance in vitamin D dependent calcium metabolism induces hyperplasia of the parathyroid which later leads to the development of a parathyroid adenoma.  In addition a review of literature describing similar cases is given."}
{"id": "PMID:1094273", "title": "Estrogen induction of ovalbumin mRNA: evidence for transcription control.", "content": "Estrogen induces the synthesis and accumulation of the specific messenger RNA for the egg white protein ovalbumin. The messenger RNA has been purified to apparent homogeneity on a preparative scale and utilized to synthesize a radioactive complementary DNA copy. This  complementary DNA probe was first used to reveal that although ovalbumin constitutes 60% of the total protein of chick oviduct the gene which codes for the ovalbumin nRNA is represented only once in each haploid genome: The induction of genetranscription and subsequent accumulation of ovalbumin mRNA during estrogen-mediated tissue differentiation was also investigated. Ovalbumin mRNA sequences were quantifiedusing the complementary DNA probe and by an in vitro heterologous translation system. Similar experiments were performed using chicks which were withdrawn from hormone treatment and then given a single injection of estrogen. The data suggest pure transcriptional control for the mechanism by which estrogen regulates the synthesis of the tissuespecific protein ovalbumin. Finally, several in vitro translation systems are comparedwith respect to their usefullness to assess the effects of hormones on mRNA production. It is concluded that the protein synthesis system derived from wheat germ offers thegreatest advantages for initial studies.", "contents": "Estrogen induction of ovalbumin mRNA: evidence for transcription control. Estrogen induces the synthesis and accumulation of the specific messenger RNA for the egg white protein ovalbumin. The messenger RNA has been purified to apparent homogeneity on a preparative scale and utilized to synthesize a radioactive complementary DNA copy. This  complementary DNA probe was first used to reveal that although ovalbumin constitutes 60% of the total protein of chick oviduct the gene which codes for the ovalbumin nRNA is represented only once in each haploid genome: The induction of genetranscription and subsequent accumulation of ovalbumin mRNA during estrogen-mediated tissue differentiation was also investigated. Ovalbumin mRNA sequences were quantifiedusing the complementary DNA probe and by an in vitro heterologous translation system. Similar experiments were performed using chicks which were withdrawn from hormone treatment and then given a single injection of estrogen. The data suggest pure transcriptional control for the mechanism by which estrogen regulates the synthesis of the tissuespecific protein ovalbumin. Finally, several in vitro translation systems are comparedwith respect to their usefullness to assess the effects of hormones on mRNA production. It is concluded that the protein synthesis system derived from wheat germ offers thegreatest advantages for initial studies."}
{"id": "PMID:1094276", "title": "Yeast mutants sensitive to trimethoprim.", "content": "Wild-type strains of Saccharomyces cerevisiae are resistant to growth inhibition by the folate antagonist trimethoprim. A mutant strain sensitive to trimethoprim was isolated. It was found to be sensitive to both ultraviolet light and X-irradiation. Genetic tests revealed that it was allelic with a known radiation-sensitive strain of Saccharomyces cerevisiae, rad 6-I. Strains harbouring a variety of mutant alleles conferring radiation-sensitivity were tested for sensitivity to trimethoprim. It was found that rad 6-I and each of the four known alleles of rad 18 conferred sensitivity to the drug, but all other rad mutants tested were trimethoprim-resistant. All trimethoprim-sensitive strains, including double mutants of rad 6 rad 18, gave rise to trimethoprim-resistant outgrowths at a rather high frequency (similar to 10-minus 5). Several resistant outgrowths were analysed. A wide variation in phenotype with respect of UV-sensitivty was found. Genetical analysis revealed that resistance to trimethoprim resulted from forward mutations at separate loci rather than back mutations of rad 6 or rad 18 alleles.", "contents": "Yeast mutants sensitive to trimethoprim. Wild-type strains of Saccharomyces cerevisiae are resistant to growth inhibition by the folate antagonist trimethoprim. A mutant strain sensitive to trimethoprim was isolated. It was found to be sensitive to both ultraviolet light and X-irradiation. Genetic tests revealed that it was allelic with a known radiation-sensitive strain of Saccharomyces cerevisiae, rad 6-I. Strains harbouring a variety of mutant alleles conferring radiation-sensitivity were tested for sensitivity to trimethoprim. It was found that rad 6-I and each of the four known alleles of rad 18 conferred sensitivity to the drug, but all other rad mutants tested were trimethoprim-resistant. All trimethoprim-sensitive strains, including double mutants of rad 6 rad 18, gave rise to trimethoprim-resistant outgrowths at a rather high frequency (similar to 10-minus 5). Several resistant outgrowths were analysed. A wide variation in phenotype with respect of UV-sensitivty was found. Genetical analysis revealed that resistance to trimethoprim resulted from forward mutations at separate loci rather than back mutations of rad 6 or rad 18 alleles."}
{"id": "PMID:1094277", "title": "Post-irradiation modifications of radiation-induced heteroallelic reversion in diploid yeast: effect of nutrient broth.", "content": "The effect of post-irradiation growth in complete rich medium on the expression of the reversion to arginine-independence induced by gamma and alpha radiation in a heteroallelic diploid yeast strain (Saccharomyces cerevisiae BZ34) has been studied. During the post-irradiation treatment the reversion frequency increased, reached a peak at about 90 min and decreased thereafter reaching a constant value for treatment periods exceeding 6 h. As determined by the increase in number of budding cells, extensive DNA synthesis took place in cells incubated only in the nutrient medium and not in the omission medium. Hence the observed increase in the reversion frequency is explained on the basis that post-irradiation DNA synthesis is necessary for the expression of gene conversion. The decrease in the reversion frequency for continued treatment with yeast extract, peptone, dextrose (YEPD) is related to the fact that only one daughter of the post-irradiation first cell division is a revertant. The broth effect was not lost when the irradiated cells were first incubated for 90 min in arginine-less medium and then transferred to the broth. Similarly, the broth effect persisted even at doses high enough to induce considerable division delay. These results suggest that the radiation-induced pre-conversional lesions are not susceptible to repair by alternative pathways.", "contents": "Post-irradiation modifications of radiation-induced heteroallelic reversion in diploid yeast: effect of nutrient broth. The effect of post-irradiation growth in complete rich medium on the expression of the reversion to arginine-independence induced by gamma and alpha radiation in a heteroallelic diploid yeast strain (Saccharomyces cerevisiae BZ34) has been studied. During the post-irradiation treatment the reversion frequency increased, reached a peak at about 90 min and decreased thereafter reaching a constant value for treatment periods exceeding 6 h. As determined by the increase in number of budding cells, extensive DNA synthesis took place in cells incubated only in the nutrient medium and not in the omission medium. Hence the observed increase in the reversion frequency is explained on the basis that post-irradiation DNA synthesis is necessary for the expression of gene conversion. The decrease in the reversion frequency for continued treatment with yeast extract, peptone, dextrose (YEPD) is related to the fact that only one daughter of the post-irradiation first cell division is a revertant. The broth effect was not lost when the irradiated cells were first incubated for 90 min in arginine-less medium and then transferred to the broth. Similarly, the broth effect persisted even at doses high enough to induce considerable division delay. These results suggest that the radiation-induced pre-conversional lesions are not susceptible to repair by alternative pathways."}
{"id": "PMID:1094279", "title": "Use of repair-deficient strains of Escherichia coli and liver microsomes to detect and characterise DNA damage caused by pyrrolizidine alkaloids heliotrine and monocrotaline.", "content": "E. coli WP2 and its repair-deficient derivatives were treated with the pyrrolizidine alkaloids, heliotrine and monocrotaline in the presence of a liver microsomal fraction. The doubly repair-deficient strains WP100 uvrA recA and CM611 uvrA exrA showed considerable killing. The singly repair-deficient strains WP2 uvrA, CM561 exrA and CM571 recA showed slight killing. In strains WP2 and WP2 uvrA induced reversion to Trp+ was not detected with either monocrotaline or mitomycin C. These results are entirely consistent with liver activation converting pyrrolizidine alkaloids into bifunctional alkylating agents.", "contents": "Use of repair-deficient strains of Escherichia coli and liver microsomes to detect and characterise DNA damage caused by pyrrolizidine alkaloids heliotrine and monocrotaline. E. coli WP2 and its repair-deficient derivatives were treated with the pyrrolizidine alkaloids, heliotrine and monocrotaline in the presence of a liver microsomal fraction. The doubly repair-deficient strains WP100 uvrA recA and CM611 uvrA exrA showed considerable killing. The singly repair-deficient strains WP2 uvrA, CM561 exrA and CM571 recA showed slight killing. In strains WP2 and WP2 uvrA induced reversion to Trp+ was not detected with either monocrotaline or mitomycin C. These results are entirely consistent with liver activation converting pyrrolizidine alkaloids into bifunctional alkylating agents."}
{"id": "PMID:1094280", "title": "Mutation and DNA replication in Escherichia coli treated with low concentrations of N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "N-Methyl-N'-nitro-N-nitrosoguanidine (nitrosoguanidine) causes an unexpectedly high frequency of closely linked double mutants because of its specificity for chromosome regions in replication. Low nitrosoguanidine concentrations (I mug/ml) in liquid cultures allow replications at the normal rate and are mutagenic. It was expected that mutations would be spread over the chromosome as it replicated, but a high frequency of closely linked double mutants was found. If a thymine auxotroph is grown in the presence of 5-bromodeoxyuridine (BUdR) and nitrosoguanidine, then exposed to 313-nm radiation (which destroys BUdR-substituted DNA), the mutation frequency is much higher among survivors than among non-irradiated cells. It is concluded that nitrosoguanidine inhibits DNA replication in a small fraction of the population and that mutations are induced in that same fraction. Nitrosoguanidine treatment leads to a high frequency of closely linked double mutants under all known conditions.", "contents": "Mutation and DNA replication in Escherichia coli treated with low concentrations of N-methyl-N'-nitro-N-nitrosoguanidine. N-Methyl-N'-nitro-N-nitrosoguanidine (nitrosoguanidine) causes an unexpectedly high frequency of closely linked double mutants because of its specificity for chromosome regions in replication. Low nitrosoguanidine concentrations (I mug/ml) in liquid cultures allow replications at the normal rate and are mutagenic. It was expected that mutations would be spread over the chromosome as it replicated, but a high frequency of closely linked double mutants was found. If a thymine auxotroph is grown in the presence of 5-bromodeoxyuridine (BUdR) and nitrosoguanidine, then exposed to 313-nm radiation (which destroys BUdR-substituted DNA), the mutation frequency is much higher among survivors than among non-irradiated cells. It is concluded that nitrosoguanidine inhibits DNA replication in a small fraction of the population and that mutations are induced in that same fraction. Nitrosoguanidine treatment leads to a high frequency of closely linked double mutants under all known conditions."}
{"id": "PMID:1094281", "title": "Ultraviolet light-induced responses of an mfd mutant of Escherichia coli B/r having a slow rate of dimer excision.", "content": "A mutant of Escherichia coli B/r designated mfd has drastically reduced ability to exhibit \"mutation frequency decline\" (MFD) the irreversible loss of potential suppressor mutations which occurs when protein synthesis is briefly inhibited after irradiation with UV. We have found that the initial rate of thymine dimer excision in the mfd mutant is only about one-third that of its mfd+ parent strain after a UV dose of 400 erg/mm-2. The yield of UV-induced Tyr+ revertants is 4-10 times higher in the mfd strain than in the mfd+ strain. This is comparable to the level of UV-mutability in the mfd+ strain in the presence of caffeine, an inhibitor of dimer excision. UV-mutability, prophage induction and Weigle reactivation of irradiated gamma phage occur to a greater extent at low UV doses (10-50 erg/mm-2) in the mfd strain compared to the mfd+ strain. We propose that the slow excision repair in the mfd mutant results in a shift in the induction threshold for these UV-inducible functions toward lower UV doses.", "contents": "Ultraviolet light-induced responses of an mfd mutant of Escherichia coli B/r having a slow rate of dimer excision. A mutant of Escherichia coli B/r designated mfd has drastically reduced ability to exhibit \"mutation frequency decline\" (MFD) the irreversible loss of potential suppressor mutations which occurs when protein synthesis is briefly inhibited after irradiation with UV. We have found that the initial rate of thymine dimer excision in the mfd mutant is only about one-third that of its mfd+ parent strain after a UV dose of 400 erg/mm-2. The yield of UV-induced Tyr+ revertants is 4-10 times higher in the mfd strain than in the mfd+ strain. This is comparable to the level of UV-mutability in the mfd+ strain in the presence of caffeine, an inhibitor of dimer excision. UV-mutability, prophage induction and Weigle reactivation of irradiated gamma phage occur to a greater extent at low UV doses (10-50 erg/mm-2) in the mfd strain compared to the mfd+ strain. We propose that the slow excision repair in the mfd mutant results in a shift in the induction threshold for these UV-inducible functions toward lower UV doses."}
{"id": "PMID:1094313", "title": "Endotoxin and acute renal failure.", "content": "The hypothesis of acute renal failure which now best fits the facts is that it is a Shwartzman equivalent, due to the multiple noxious effects of endotoxin. Endotoxin alone can account for all those separate factors which are known to cause an increase of the renal vascular resistance. The discrepancies and the implications are discussed.", "contents": "Endotoxin and acute renal failure. The hypothesis of acute renal failure which now best fits the facts is that it is a Shwartzman equivalent, due to the multiple noxious effects of endotoxin. Endotoxin alone can account for all those separate factors which are known to cause an increase of the renal vascular resistance. The discrepancies and the implications are discussed."}
{"id": "PMID:1094314", "title": "Effects of large doses of furosemide in end-stage chronic renal failure.", "content": "Administered orally at 500 mg/day to patients in end-stage chronic renal failure treated by hemodialysis, furosemide significantly decreases the weight gain between dialyses and increases sodium excretion rate. This therapy remains without effect on blood pressure, creatinine clearance and urinary excretion of potassium and nitrogen. Devoid of any side-effects, at least during short periods, the treatment may contribute to achieve better control of sodium and water balance.", "contents": "Effects of large doses of furosemide in end-stage chronic renal failure. Administered orally at 500 mg/day to patients in end-stage chronic renal failure treated by hemodialysis, furosemide significantly decreases the weight gain between dialyses and increases sodium excretion rate. This therapy remains without effect on blood pressure, creatinine clearance and urinary excretion of potassium and nitrogen. Devoid of any side-effects, at least during short periods, the treatment may contribute to achieve better control of sodium and water balance."}
{"id": "PMID:1094316", "title": "Effect of castration upon hypothalamic luteinizing hormone releasing factor (LH-RF).", "content": "The effect of castration upon hypothalamic LH-RF synthesis and content was determined in mature male rats. In each single experiment 20 hypothalami from both normal and 60-day-castrated rats were bisected into symmetrical portions. For the determination of LH-RF synthesis 20 hypothalamic halves were incubated for 120 min. After incubation the tissue was homogenized in medium containing 1 N acetic acid, and centrifuged; 1.0 ml of the supernatant was used to test LH-RF activity. In the other 20 halves, hypothalamic LH-RF content was determined: the samples were homogenized without incubation in 1 N acetic acid, centrifuged, and LH-RF activity was tested in 1.0 ml of the supernatant. LH-RF activity was determined in vivo in ovariectomized rats pre-treated with estrogen-progesterone by measuring, by radioimmunoassay, the LH modifications in serum after the i.v. administration of the extracts. Hypothalamic LH-RF content was significantly higher in control than in castrated rats (p is less than 0.001). After incubation there were no changes in the LH-releasing potency of control hypothalami compared with non-incubated tissue, whereas a significant increase (616%) was found after the incubation of hypothalami from castrated rats. On the other hand, after incubation LH-RF activity was higher (p is less than 0.02) in castrated than in control hypothalami. These results suggest that castration stimulates the release and synthesis of hypothalamic LH-RF in male rats.", "contents": "Effect of castration upon hypothalamic luteinizing hormone releasing factor (LH-RF). The effect of castration upon hypothalamic LH-RF synthesis and content was determined in mature male rats. In each single experiment 20 hypothalami from both normal and 60-day-castrated rats were bisected into symmetrical portions. For the determination of LH-RF synthesis 20 hypothalamic halves were incubated for 120 min. After incubation the tissue was homogenized in medium containing 1 N acetic acid, and centrifuged; 1.0 ml of the supernatant was used to test LH-RF activity. In the other 20 halves, hypothalamic LH-RF content was determined: the samples were homogenized without incubation in 1 N acetic acid, centrifuged, and LH-RF activity was tested in 1.0 ml of the supernatant. LH-RF activity was determined in vivo in ovariectomized rats pre-treated with estrogen-progesterone by measuring, by radioimmunoassay, the LH modifications in serum after the i.v. administration of the extracts. Hypothalamic LH-RF content was significantly higher in control than in castrated rats (p is less than 0.001). After incubation there were no changes in the LH-releasing potency of control hypothalami compared with non-incubated tissue, whereas a significant increase (616%) was found after the incubation of hypothalami from castrated rats. On the other hand, after incubation LH-RF activity was higher (p is less than 0.02) in castrated than in control hypothalami. These results suggest that castration stimulates the release and synthesis of hypothalamic LH-RF in male rats."}
{"id": "PMID:1094317", "title": "First Geoffrey Harris Memorial lecture. Some recent developments in brain-pituitary-ovarian physiology.", "content": "Figure 14 summarizes some of the brain-pituitary-ovarian relationships which we have been discussing. Neurons in the arcuate nucleus of the hypophysiotropic area produce LRH which is secreted into the proximal capillary plexus of the hypophysio-portal system in the median eminence. LRH production is modulated in the rat and possibly also in the monkey by direct input from the preoptic region and less directly by facilitatory and inhibitory projections from the amygdala, hippocampus and midbrain. Electrophysiological evidence has been presented for \"ultrashort' feedback action of LRH on arcuate neurons as well as the \"short' feedback loop of LH. Gonadal steroids are pictured as feeding back directly to the pituitary and to several sites in the brain where positive and negative influences are exerted on both pituitary-ovarian function and sexual behavior. Evidence has been presented that adrenergic mechanisms influence LRH release with norepinephrine facilitatory and dopamine inhibitory to the process; dopaminergic neurons may be more intimately related to the control of prolactin secretion, and some consider dopamine and prolactin inhibiting factor to be identical. Finally, immunological methods as represented by immunohistochemistry and radioimmunoassay promise further important developments in this field in the near future. I am sure that Geoffrey Harris would have been pleased with the progress made in answering the questions which he raised in his Dale lecture.", "contents": "First Geoffrey Harris Memorial lecture. Some recent developments in brain-pituitary-ovarian physiology. Figure 14 summarizes some of the brain-pituitary-ovarian relationships which we have been discussing. Neurons in the arcuate nucleus of the hypophysiotropic area produce LRH which is secreted into the proximal capillary plexus of the hypophysio-portal system in the median eminence. LRH production is modulated in the rat and possibly also in the monkey by direct input from the preoptic region and less directly by facilitatory and inhibitory projections from the amygdala, hippocampus and midbrain. Electrophysiological evidence has been presented for \"ultrashort' feedback action of LRH on arcuate neurons as well as the \"short' feedback loop of LH. Gonadal steroids are pictured as feeding back directly to the pituitary and to several sites in the brain where positive and negative influences are exerted on both pituitary-ovarian function and sexual behavior. Evidence has been presented that adrenergic mechanisms influence LRH release with norepinephrine facilitatory and dopamine inhibitory to the process; dopaminergic neurons may be more intimately related to the control of prolactin secretion, and some consider dopamine and prolactin inhibiting factor to be identical. Finally, immunological methods as represented by immunohistochemistry and radioimmunoassay promise further important developments in this field in the near future. I am sure that Geoffrey Harris would have been pleased with the progress made in answering the questions which he raised in his Dale lecture."}
{"id": "PMID:1094318", "title": "Clinical evaluation of eterobarb, a new anticonvulsant drug.", "content": "Two clinical investigations of a new anticonvulsant, eterobarb, N,N' dimethoxymethyl phenobarbital (DMMP), were conducted in separate geographic regions. The drug has considerable efficacy in reducing the frequency of partial seizures, with and without secondary generalization, as well as generalized tonic-clonic seizures. Sedation did not appear to be as prominent with this barbiturate as it was with phenobarbital or primidone.", "contents": "Clinical evaluation of eterobarb, a new anticonvulsant drug. Two clinical investigations of a new anticonvulsant, eterobarb, N,N' dimethoxymethyl phenobarbital (DMMP), were conducted in separate geographic regions. The drug has considerable efficacy in reducing the frequency of partial seizures, with and without secondary generalization, as well as generalized tonic-clonic seizures. Sedation did not appear to be as prominent with this barbiturate as it was with phenobarbital or primidone."}
{"id": "PMID:1094319", "title": "Tubular aggregates in subclinical alcoholic myopathy.", "content": "A 34-year-old chronic alcoholic with acute alcoholic intoxication was found to have extensive aggregates on muscle biopsy performed 48 hours after admission. Forearm ischemic exercise failed to demonstrate normal generation of lactic acid. Pathologic changes in the muscle biopsy consisted of subsarcolemmal accumulations of bright purple-red material with trichrome reaction. This material stained darkly with NADH-TR but was unstained with myofibrillar ATPase and Pas. Ultrastructural studies revealed that these regions contained tubular aggregates. A second biopsy 7 days later failed to demonstrate any significant abnormalities. Two weeks later, lactate generation was normal. Previous observations by other authors that tubular aggregates may be concerned with correction of metabolic defect or detoxification of endogenous toxins could apply in our case.", "contents": "Tubular aggregates in subclinical alcoholic myopathy. A 34-year-old chronic alcoholic with acute alcoholic intoxication was found to have extensive aggregates on muscle biopsy performed 48 hours after admission. Forearm ischemic exercise failed to demonstrate normal generation of lactic acid. Pathologic changes in the muscle biopsy consisted of subsarcolemmal accumulations of bright purple-red material with trichrome reaction. This material stained darkly with NADH-TR but was unstained with myofibrillar ATPase and Pas. Ultrastructural studies revealed that these regions contained tubular aggregates. A second biopsy 7 days later failed to demonstrate any significant abnormalities. Two weeks later, lactate generation was normal. Previous observations by other authors that tubular aggregates may be concerned with correction of metabolic defect or detoxification of endogenous toxins could apply in our case."}
{"id": "PMID:1094320", "title": "9-Alpha-fluorohydrocortisone in the treatment of postural hypotension in diabetic autonomic neuropathy.", "content": "A double-blind crossover study of fludrocortisone, 0,1 mg. twice daily, and placebo is reported in six diabetics with troublesome symptoms of postural hypotension due to autonomic neuropathy. During treatment with the active preparation there was an increase in the lying and tilted systolic blood pressure, a decrease in orthostatic tachycardia, and in increase in the total plasma volume and body weight, but with no change in plasma or urine osmolality; The symptoms of postural hypotension improved in four patients, while two patients with a low serum albumin developed ankle edema during treatment with fludrocortisone. It is concluded that fludrocortisone is effective in diabetics with symptomatic postural hypotesnion, but should be used with caution in patients with a low serum albumin.", "contents": "9-Alpha-fluorohydrocortisone in the treatment of postural hypotension in diabetic autonomic neuropathy. A double-blind crossover study of fludrocortisone, 0,1 mg. twice daily, and placebo is reported in six diabetics with troublesome symptoms of postural hypotension due to autonomic neuropathy. During treatment with the active preparation there was an increase in the lying and tilted systolic blood pressure, a decrease in orthostatic tachycardia, and in increase in the total plasma volume and body weight, but with no change in plasma or urine osmolality; The symptoms of postural hypotension improved in four patients, while two patients with a low serum albumin developed ankle edema during treatment with fludrocortisone. It is concluded that fludrocortisone is effective in diabetics with symptomatic postural hypotesnion, but should be used with caution in patients with a low serum albumin."}
{"id": "PMID:1094321", "title": "Historical perspective to a current controversy on the clinical spectrum of plumbism.", "content": "Concepts of health risk associated with lead usage have undergone profound change. In the past observations of the relationship between lead usage and lead poisoning were severely limited by lack of knowledge and the relatively small quantity of the metal used. The benefits of lead usage, when contrasted with the risk incurred, outweighed the known hazards. Industrial growth contributed to the increased interest in lead poisoning. A significant increase of workers at risk generated new interest in an old disease. A concept of control developed based upon the principle of a dose-response relationship. The old idea of benefit versus risk remained because technological society needed lead but agreed that by controlling the factory environment lead could be used safely. The criteria for injury remained climical plumbism. Growth and change in lead utilization, once again, brought lead hazard to the forefront. New applications distributed lead throughout the environment exposiing both workers and the general population. A segment of the scientific community now suggests that the old criteria to judge risk, clinical symptoms of plumbism, are inadequate.", "contents": "Historical perspective to a current controversy on the clinical spectrum of plumbism. Concepts of health risk associated with lead usage have undergone profound change. In the past observations of the relationship between lead usage and lead poisoning were severely limited by lack of knowledge and the relatively small quantity of the metal used. The benefits of lead usage, when contrasted with the risk incurred, outweighed the known hazards. Industrial growth contributed to the increased interest in lead poisoning. A significant increase of workers at risk generated new interest in an old disease. A concept of control developed based upon the principle of a dose-response relationship. The old idea of benefit versus risk remained because technological society needed lead but agreed that by controlling the factory environment lead could be used safely. The criteria for injury remained climical plumbism. Growth and change in lead utilization, once again, brought lead hazard to the forefront. New applications distributed lead throughout the environment exposiing both workers and the general population. A segment of the scientific community now suggests that the old criteria to judge risk, clinical symptoms of plumbism, are inadequate."}
{"id": "PMID:1094348", "title": "Klebsiella urinary tract infection.", "content": "The Klebsiella species form a heterogeneous group of gram negative, lactose fermenting, encapsulated, non-motile bacilli. They are important urinary tract pathogens, especially in long stay hospital patients and infection is often associated with urethral catheterisation. The laboratory characterisation of these organisms by biochemical means is time consuming and not entirely satisfactory. Antibiotic sensitivity tests indicate that gentamicin is the drug of choice for treatment of a urinary tract infection due to klebsiella.", "contents": "Klebsiella urinary tract infection. The Klebsiella species form a heterogeneous group of gram negative, lactose fermenting, encapsulated, non-motile bacilli. They are important urinary tract pathogens, especially in long stay hospital patients and infection is often associated with urethral catheterisation. The laboratory characterisation of these organisms by biochemical means is time consuming and not entirely satisfactory. Antibiotic sensitivity tests indicate that gentamicin is the drug of choice for treatment of a urinary tract infection due to klebsiella."}
{"id": "PMID:1094349", "title": "Gastric ulcer controlled trial using a licorice derivative rucedal.", "content": "A controlled trial of a licorice derivative, Rucedal, used in the management of gastric ulcer did not hasten the healing of the ulcer over eight weeks.", "contents": "Gastric ulcer controlled trial using a licorice derivative rucedal. A controlled trial of a licorice derivative, Rucedal, used in the management of gastric ulcer did not hasten the healing of the ulcer over eight weeks."}
{"id": "PMID:1094364", "title": "Combined Kelman procedure (phaco-emulsification cataract extraction) and simultaneous aphakic penetrating keratoplasty.", "content": "The problem  of management of coexistent lenticular and corneal opacification is, as yet, unresolved. Combined intracapsular cataract extraction and penetrating keratoplasty affords the risk of vitreous loss at the time of surgery or perhaps the even greater risk of vitreous adhesion to the donor cornea. Leaving an intact posterior capsule would certainly avoid the problems of vitreous touch. Where applicable, we have been utilizing the technique of combined phaco-emulsification cataract extraction followed by aphakic penetrating keratoplasty leaving an intact posterior capsule and removing all cortical remnants with the irrigation-aspiration probe of the Kelman Cavitron unit. This technique has proved extremely useful in our hands and we suggest that it is a useful alternative in the management of coexistent lenticular and corneal opacification.", "contents": "Combined Kelman procedure (phaco-emulsification cataract extraction) and simultaneous aphakic penetrating keratoplasty. The problem  of management of coexistent lenticular and corneal opacification is, as yet, unresolved. Combined intracapsular cataract extraction and penetrating keratoplasty affords the risk of vitreous loss at the time of surgery or perhaps the even greater risk of vitreous adhesion to the donor cornea. Leaving an intact posterior capsule would certainly avoid the problems of vitreous touch. Where applicable, we have been utilizing the technique of combined phaco-emulsification cataract extraction followed by aphakic penetrating keratoplasty leaving an intact posterior capsule and removing all cortical remnants with the irrigation-aspiration probe of the Kelman Cavitron unit. This technique has proved extremely useful in our hands and we suggest that it is a useful alternative in the management of coexistent lenticular and corneal opacification."}
{"id": "PMID:1094366", "title": "Primary reticulum-cell sarcoma of the mandible. Review of the literature and report of a case.", "content": "A 52-year-old man developed intermittent swelling, pain, and paresthesia during a one-year period prior to the histologic diagnosis of reticulum-cell sarcoma. The clinical profile and radiographic examination suggested either chronic osteomyelitis or malignancy. Treatment was confirmed to radiation therapy with resolution of the malignancy but a guarded prognosis. Twenty-two previously reported cases of primary reticulum-cell sarcoma of the mandible are reviewed.", "contents": "Primary reticulum-cell sarcoma of the mandible. Review of the literature and report of a case. A 52-year-old man developed intermittent swelling, pain, and paresthesia during a one-year period prior to the histologic diagnosis of reticulum-cell sarcoma. The clinical profile and radiographic examination suggested either chronic osteomyelitis or malignancy. Treatment was confirmed to radiation therapy with resolution of the malignancy but a guarded prognosis. Twenty-two previously reported cases of primary reticulum-cell sarcoma of the mandible are reviewed."}
{"id": "PMID:1094367", "title": "Treatment of labyrinthine fistulae by a closed technique.", "content": "A fistula on the lateral semicircular canal was found in 14 (4.8%) ears with cholesteatoma. In 11 patients with preserved inner-ear function the cholesteatoma membrane was removed, the fistula was solidly closed with fascia, bone, and again fascia. The cavity was obliterated, the meatal wall reconstructed, and tympanoplasty performed in the same stage. Ten patients obtained a hearing gain, an average of 16.8 dB, while one developed anacusis. The results show that the cholesteatoma matrix can be removed from most fistulae. In the case of large fistulae, in which the cholesteatoma matrix is adhering to or invading the fistula, there is a great risk of perceptive hearing loss. By closing the fistula with fascia and bone and using a closed technique a lasting hearing gain may be achieved.", "contents": "Treatment of labyrinthine fistulae by a closed technique. A fistula on the lateral semicircular canal was found in 14 (4.8%) ears with cholesteatoma. In 11 patients with preserved inner-ear function the cholesteatoma membrane was removed, the fistula was solidly closed with fascia, bone, and again fascia. The cavity was obliterated, the meatal wall reconstructed, and tympanoplasty performed in the same stage. Ten patients obtained a hearing gain, an average of 16.8 dB, while one developed anacusis. The results show that the cholesteatoma matrix can be removed from most fistulae. In the case of large fistulae, in which the cholesteatoma matrix is adhering to or invading the fistula, there is a great risk of perceptive hearing loss. By closing the fistula with fascia and bone and using a closed technique a lasting hearing gain may be achieved."}
{"id": "PMID:1094368", "title": "Therapeutic problems in laryngo-tracheal stenoses.", "content": "History revealed iatrogenic injury (tracheostomy, intubation, artificial respiration) to be the reason for tracheal stenosis in each of our 32 cases (18 female, 14 male). So far 23 cases have been treated successfully and they live without a cannula; seven are still under treatment, while two patients died (severe heart-attack, spontaneous pneumothorax) and one refused further treatment. We were relatively successful in six cases of sleeve resection. All other 26 cases were treated by creating a tracheal groove, in which tracheal mucosa and skin of the neck were joined and the caudal end mostly kept open by using a pedicle skin flap. Intrathoracic stenoses require a sternotomy. An additional thyroidectomy and lateral traction to the tracheal walls by using loops out of the sternomastoid muscle became necessary in many cases and we obligatorily used tamponades of plastic inlay bolts, the average time of treatment being 8 months.", "contents": "Therapeutic problems in laryngo-tracheal stenoses. History revealed iatrogenic injury (tracheostomy, intubation, artificial respiration) to be the reason for tracheal stenosis in each of our 32 cases (18 female, 14 male). So far 23 cases have been treated successfully and they live without a cannula; seven are still under treatment, while two patients died (severe heart-attack, spontaneous pneumothorax) and one refused further treatment. We were relatively successful in six cases of sleeve resection. All other 26 cases were treated by creating a tracheal groove, in which tracheal mucosa and skin of the neck were joined and the caudal end mostly kept open by using a pedicle skin flap. Intrathoracic stenoses require a sternotomy. An additional thyroidectomy and lateral traction to the tracheal walls by using loops out of the sternomastoid muscle became necessary in many cases and we obligatorily used tamponades of plastic inlay bolts, the average time of treatment being 8 months."}
{"id": "PMID:1094390", "title": "A new pattern mouth stick.", "content": "The motivation for the severely handicapped tetraplegic patient in using the mouth stick is quite considerable as through this simple oral aid they are able to carry out small jobs and be involved in social activities. We feel that this modified pattern mouth stick fulfils the aims which we require of it.", "contents": "A new pattern mouth stick. The motivation for the severely handicapped tetraplegic patient in using the mouth stick is quite considerable as through this simple oral aid they are able to carry out small jobs and be involved in social activities. We feel that this modified pattern mouth stick fulfils the aims which we require of it."}
{"id": "PMID:1094393", "title": "Use of antiserum agar for detection of Haemophilus influenzae type b in the pharynx.", "content": "An antiserum agar medium was found to provide a rapid, sensitive, and highly specific method for pharyngeal culture and quantitation of HIB.", "contents": "Use of antiserum agar for detection of Haemophilus influenzae type b in the pharynx. An antiserum agar medium was found to provide a rapid, sensitive, and highly specific method for pharyngeal culture and quantitation of HIB."}
{"id": "PMID:1094399", "title": "Chronic pulmonary disease in neonates with assisted ventilation.", "content": "Chronic lung disease in the form of stage III or IV bronchopulmonary dysplasia (BPD) was found to occur among nine of 188 infants receiving intermittent positive-pressure ventilation for respiratory insufficiency. None occurred in infants ventilated with a mask alone. The duration of endotracheal intubation was not significantly longer in patients developing BPD; however, the duration of exposure to inspired oxygen greater than 60 percent was significantly longer in that group (mean, 141 hours). A previously undescribed form of chronic lung disease was found to occur among nine of 150 infants ventilated because of underlying idiopathic respiratory distress syndrome. A radiographic appearance of diffuse haziness with loss of identifiable lung markings occurred at 5 to 15 days of age and was not associated with clinical signs or symptoms or increased oxygen needs. These changes disappeared in one to five days.", "contents": "Chronic pulmonary disease in neonates with assisted ventilation. Chronic lung disease in the form of stage III or IV bronchopulmonary dysplasia (BPD) was found to occur among nine of 188 infants receiving intermittent positive-pressure ventilation for respiratory insufficiency. None occurred in infants ventilated with a mask alone. The duration of endotracheal intubation was not significantly longer in patients developing BPD; however, the duration of exposure to inspired oxygen greater than 60 percent was significantly longer in that group (mean, 141 hours). A previously undescribed form of chronic lung disease was found to occur among nine of 150 infants ventilated because of underlying idiopathic respiratory distress syndrome. A radiographic appearance of diffuse haziness with loss of identifiable lung markings occurred at 5 to 15 days of age and was not associated with clinical signs or symptoms or increased oxygen needs. These changes disappeared in one to five days."}
{"id": "PMID:1094400", "title": "Occult spinal dysraphism: a series of 73 cases.", "content": "The subject of occult spinal dysraphism or myelodysplasia is reviewed from standpoints of embryology, clinical manifestations, and treatment, and the management of 73 cases summarized. In general, these concealed lesions arise from developmental variants in the most distal part of the neural tube, a situation which may cause distortion or partial absence of neural tissues and also lead to damage from compression or traction. Lipomyelomeningocele and congenital dermal sinus are two exampled of the many types of such lesions, but some are more complicated and border-line myelomeningocele-like forms occur. Incontinence, deformity or weakness of the feet, impaired gait, and other difficulties may appear late and increase with growth. Surgical treatment is advised to reduce chances of delayed or progressive loss of function.", "contents": "Occult spinal dysraphism: a series of 73 cases. The subject of occult spinal dysraphism or myelodysplasia is reviewed from standpoints of embryology, clinical manifestations, and treatment, and the management of 73 cases summarized. In general, these concealed lesions arise from developmental variants in the most distal part of the neural tube, a situation which may cause distortion or partial absence of neural tissues and also lead to damage from compression or traction. Lipomyelomeningocele and congenital dermal sinus are two exampled of the many types of such lesions, but some are more complicated and border-line myelomeningocele-like forms occur. Incontinence, deformity or weakness of the feet, impaired gait, and other difficulties may appear late and increase with growth. Surgical treatment is advised to reduce chances of delayed or progressive loss of function."}
{"id": "PMID:1094401", "title": "Recurrent Caffey's cortical hyperostosis and persistent deformity.", "content": "A boy, age 12 years, who had infantile cortical hyperostosis has continued to have occasional aches and new cortical thickenings in his arms and legs. His mandible is undergrown and his ribs have an abnormal slope. Recurrence of Caffey's cortical hyperostosis and persistent deformity have been observed in other children and young adults. Some unexplained cases of late cortical thickening and pain may be due to Caffey's disease.", "contents": "Recurrent Caffey's cortical hyperostosis and persistent deformity. A boy, age 12 years, who had infantile cortical hyperostosis has continued to have occasional aches and new cortical thickenings in his arms and legs. His mandible is undergrown and his ribs have an abnormal slope. Recurrence of Caffey's cortical hyperostosis and persistent deformity have been observed in other children and young adults. Some unexplained cases of late cortical thickening and pain may be due to Caffey's disease."}
{"id": "PMID:1094402", "title": "Myelofibrosis-myeloid metaplasia in childhood.", "content": "A child with idiopathic myeloid metaplasia is described and her course contrasted with another child having myeloid metaplasia secondary to reticulum cell sarcoma. Despite prior reports the idiopathic form of illness may pursue a relatively indolent course in children. A thorough search should be made for underlying disease when myeloid metaplasia is first diagnosed.", "contents": "Myelofibrosis-myeloid metaplasia in childhood. A child with idiopathic myeloid metaplasia is described and her course contrasted with another child having myeloid metaplasia secondary to reticulum cell sarcoma. Despite prior reports the idiopathic form of illness may pursue a relatively indolent course in children. A thorough search should be made for underlying disease when myeloid metaplasia is first diagnosed."}
{"id": "PMID:1094405", "title": "[Persistance of resistant Escherichia coli in a calf herd].", "content": "In an experimental station for calf feeding the occurrence and distribution of drug resistant E. coli in faeces have been investigated among selected groups of calves. Samples were obatined just after the calves arrived 1--2 weeks old in January--February and at intervals until an age of 7--11 months. Daily from arrival to the age of 8 weeks all calves at the station received antibiotics in their feed. In 1971 it was chlortetracycline and in 1972 zincbacitracin. In addition, from 3.5 percent to 60 percent of all calves were every month given therapeutic doses of antibiotics, mainly penicillin + streptomycin and neomycin. Both years the highest number of treatments were administered in the months of February and March (cf. fig. 1). In each of the years studied, a high prevalence of calves harbouring resistant E. coli (total resistance: greater than 88 percent) and R-+E. coli (1971: 83 percent and 1972: 50 percent) was demonstrated at the first sampling. As the calves grew older a general tendency to exchange the resistant strains with sensitive ones was recognized. The prevalence of calves with R-+ E. coli generally devreased at a faster rate than that of total resistance. A discussion is given of the epidemiological significance of cross infection and of selection pressure of antibiotics for the establishment of a resistant flora in individuals as well as for replacement of this flora with sensitive strains.", "contents": "[Persistance of resistant Escherichia coli in a calf herd]. In an experimental station for calf feeding the occurrence and distribution of drug resistant E. coli in faeces have been investigated among selected groups of calves. Samples were obatined just after the calves arrived 1--2 weeks old in January--February and at intervals until an age of 7--11 months. Daily from arrival to the age of 8 weeks all calves at the station received antibiotics in their feed. In 1971 it was chlortetracycline and in 1972 zincbacitracin. In addition, from 3.5 percent to 60 percent of all calves were every month given therapeutic doses of antibiotics, mainly penicillin + streptomycin and neomycin. Both years the highest number of treatments were administered in the months of February and March (cf. fig. 1). In each of the years studied, a high prevalence of calves harbouring resistant E. coli (total resistance: greater than 88 percent) and R-+E. coli (1971: 83 percent and 1972: 50 percent) was demonstrated at the first sampling. As the calves grew older a general tendency to exchange the resistant strains with sensitive ones was recognized. The prevalence of calves with R-+ E. coli generally devreased at a faster rate than that of total resistance. A discussion is given of the epidemiological significance of cross infection and of selection pressure of antibiotics for the establishment of a resistant flora in individuals as well as for replacement of this flora with sensitive strains."}
{"id": "PMID:1094406", "title": "[Antibiotic resistance and transferable antibiotic resistance of Escherichia coli isolated from Swedish calves 5 and 30 days old].", "content": "E. coli strains isolated from 5-day-old and 30-day-old healthy calves were tested for antibiotic resistance and H-factor mediated antibiotic resistance. An average of 1.6 antibiotic-resistant strains and 1.1 strains with transferable antibiotic resistance were isolated from each of the investigated calves. In comparison with the 30-day-old calves, the 5-day-old calves had significantly more strains with transferable antibiotic resistance (95.8 percent as against 63.4 percent). The R+ strains isolated from the younger calves transferred significantly more en bloc (43.5 percent as against 10.0 percent) and double plus multiple resistance (5292 percent as against 24.4 percent) than did those isolated from the older calves. The most common resistance was to sulphonamide and tetracycline and the most common transferred resistance was to sulphonamide.", "contents": "[Antibiotic resistance and transferable antibiotic resistance of Escherichia coli isolated from Swedish calves 5 and 30 days old]. E. coli strains isolated from 5-day-old and 30-day-old healthy calves were tested for antibiotic resistance and H-factor mediated antibiotic resistance. An average of 1.6 antibiotic-resistant strains and 1.1 strains with transferable antibiotic resistance were isolated from each of the investigated calves. In comparison with the 30-day-old calves, the 5-day-old calves had significantly more strains with transferable antibiotic resistance (95.8 percent as against 63.4 percent). The R+ strains isolated from the younger calves transferred significantly more en bloc (43.5 percent as against 10.0 percent) and double plus multiple resistance (5292 percent as against 24.4 percent) than did those isolated from the older calves. The most common resistance was to sulphonamide and tetracycline and the most common transferred resistance was to sulphonamide."}
{"id": "PMID:1094407", "title": "Preweaning mortality in pigs. 4 Diseases of the gastrointestinal tract in pigs.", "content": "The incidence of fatal gastroenteropathies in sucking pigs was studied during a 2-year period in 17 sow herds involving 2,936 litters. The results showed that 2.8 per cent of the liveborn pigs died with gastroenteropathies during the sucking and immediate post weaning period. The incidence was lowest in offspring from second-litter sows (Table I) and the progeny from large litters tended to have the highest incidence (Table II). Fatal gastrointestinal diseases were observed in a total of 17.6 per cent of the examined litters, and the average number of death per affected litter was 1.5 (Tables III and IV). The progeny of sows with post parturient diseases had significantly higher death losses than had the progeny from normal healthy sows (Table V). Climate had no apparent influence on death losses, but they were lowest during the warmer period of the year (April-September) (Tables VIII and VI.) The incidence varied considerably from herd to herd, but was not influenced by herd size (Table III). However, the level of hygiene exerted a pronounced influence on the incidence (Table VII), and self-contained herds had significantly lower death losses than had herds where female breeding stock was occasionally brought in (Table IX). Approximately 65 per cent of the pigs which succumbed due to gastrointestinal diseases died during the first week of life, which means that 1.8 per cent of the pigs at risk died during this period (Table X). The material was grouped according to the results of the post mortem and bacteriological examinations (Table XI). Approximately 50 per cent of the fatal cases were associated with an intestinal bacterial infection, which in most instances was caused by pathogenic strains of E. coli (Tables XII and XIII), while approximately 14 per cent had distinct gross pathological lesions which alone were indication of a diagnosis. In the remaining one third of the fatal cases the aetiology and pathogenesis remained by and large unexplained; however, nutritional and dietetic disorders played a role in many of these cases. Aetiological, epidemiological and pathological aspects of the fatal gastrointestinal diseases are discussed, and it is concluded that a high level of hygiene, the prevention and treatment of post parturient diseases, \"closed\" management systems, and the avoidance of unsuitable or damaged sow feed would be instrumental in keeping the level of fatal gastrointestinal diseases low among sucking pigs.", "contents": "Preweaning mortality in pigs. 4 Diseases of the gastrointestinal tract in pigs. The incidence of fatal gastroenteropathies in sucking pigs was studied during a 2-year period in 17 sow herds involving 2,936 litters. The results showed that 2.8 per cent of the liveborn pigs died with gastroenteropathies during the sucking and immediate post weaning period. The incidence was lowest in offspring from second-litter sows (Table I) and the progeny from large litters tended to have the highest incidence (Table II). Fatal gastrointestinal diseases were observed in a total of 17.6 per cent of the examined litters, and the average number of death per affected litter was 1.5 (Tables III and IV). The progeny of sows with post parturient diseases had significantly higher death losses than had the progeny from normal healthy sows (Table V). Climate had no apparent influence on death losses, but they were lowest during the warmer period of the year (April-September) (Tables VIII and VI.) The incidence varied considerably from herd to herd, but was not influenced by herd size (Table III). However, the level of hygiene exerted a pronounced influence on the incidence (Table VII), and self-contained herds had significantly lower death losses than had herds where female breeding stock was occasionally brought in (Table IX). Approximately 65 per cent of the pigs which succumbed due to gastrointestinal diseases died during the first week of life, which means that 1.8 per cent of the pigs at risk died during this period (Table X). The material was grouped according to the results of the post mortem and bacteriological examinations (Table XI). Approximately 50 per cent of the fatal cases were associated with an intestinal bacterial infection, which in most instances was caused by pathogenic strains of E. coli (Tables XII and XIII), while approximately 14 per cent had distinct gross pathological lesions which alone were indication of a diagnosis. In the remaining one third of the fatal cases the aetiology and pathogenesis remained by and large unexplained; however, nutritional and dietetic disorders played a role in many of these cases. Aetiological, epidemiological and pathological aspects of the fatal gastrointestinal diseases are discussed, and it is concluded that a high level of hygiene, the prevention and treatment of post parturient diseases, \"closed\" management systems, and the avoidance of unsuitable or damaged sow feed would be instrumental in keeping the level of fatal gastrointestinal diseases low among sucking pigs."}
{"id": "PMID:1094408", "title": "Preweaning mortality in pigs. 5. acute septicaemias.", "content": "A 2-year study on preweaning mortality revealed that 2.1 per cent of the liveborn pigs succumbed to acute bacterial septicaemias. The most frequently encountered causative organisms were haemolytic streptococci and E. coli.", "contents": "Preweaning mortality in pigs. 5. acute septicaemias. A 2-year study on preweaning mortality revealed that 2.1 per cent of the liveborn pigs succumbed to acute bacterial septicaemias. The most frequently encountered causative organisms were haemolytic streptococci and E. coli."}
{"id": "PMID:1094413", "title": "[Uricolytic therapy. Value of urate oxidase in the treatment of hyperuricemias].", "content": "In addition to uricosuric agents and purine synthesis inhibitors, Urate Oxydase may be used in the treatment of hyperuricaemia. This substance breaks down uric acid to allantoin which is soluble and easily excreted. Use of the medication in 14 cases of major gout with tophi and 9 cases of hyperuricaemia secondary to renal insufficiency showed the good tolerance of the substance and it's clinical and biological effectiveness.", "contents": "[Uricolytic therapy. Value of urate oxidase in the treatment of hyperuricemias]. In addition to uricosuric agents and purine synthesis inhibitors, Urate Oxydase may be used in the treatment of hyperuricaemia. This substance breaks down uric acid to allantoin which is soluble and easily excreted. Use of the medication in 14 cases of major gout with tophi and 9 cases of hyperuricaemia secondary to renal insufficiency showed the good tolerance of the substance and it's clinical and biological effectiveness."}
{"id": "PMID:1094414", "title": "[Determination of myocardial contractility using ultrasonic echography].", "content": "Ultrasonic echography makes possible the recording of movements of the septum and posterior wall of the left ventricle. On graphic records of good quality it is thus possible to measure accurately the transverse diameter of the left ventricle throughout systole and to determine the average and maximal speeds of decreases in this diameter, which are proportional to the VCF. Echography, a non-invasive technique, thus gives data with regard to myocardial contractility of similar value to the information provided by cineangiocardiography. In those cases where it is not possible to obtain such tracings, an approximation may be made, validity of which appears to be confirmed by clinical experience.", "contents": "[Determination of myocardial contractility using ultrasonic echography]. Ultrasonic echography makes possible the recording of movements of the septum and posterior wall of the left ventricle. On graphic records of good quality it is thus possible to measure accurately the transverse diameter of the left ventricle throughout systole and to determine the average and maximal speeds of decreases in this diameter, which are proportional to the VCF. Echography, a non-invasive technique, thus gives data with regard to myocardial contractility of similar value to the information provided by cineangiocardiography. In those cases where it is not possible to obtain such tracings, an approximation may be made, validity of which appears to be confirmed by clinical experience."}
{"id": "PMID:1094419", "title": "Detection of cation-specific conformational changes in ribosomal RNA by gel electrophoresis.", "content": "Electrophoresis of ribosomal RNA in polyacrylamide-agrose composite gels separates 16S and 23S species into multiple bands. These bands of RNA represent multiple conformational forms of the molecules as judged by oligonucleotide analysis of the 16S RNA. Gel elctrophoresis was used to test for cation-specific conformational changes in ribosomal RNA. Relative to magnesium-equilibrated RNA, barium ion and putrescine induced alterations in the electrophoretic behavior of ribosomal RNA while calcium ion produced no change. Exchange of a critical level of bound magnesium ion for barium or putrescine was necessary for these changes to take place. The alterations in electrophoretic behavior were unaffected by simply restoring magnesium ion, but in addition required heating for reversal. We suggest that these conformational changes are a result of interaction at a specific class of cation binding sites previously observed with intact ribosomes.", "contents": "Detection of cation-specific conformational changes in ribosomal RNA by gel electrophoresis. Electrophoresis of ribosomal RNA in polyacrylamide-agrose composite gels separates 16S and 23S species into multiple bands. These bands of RNA represent multiple conformational forms of the molecules as judged by oligonucleotide analysis of the 16S RNA. Gel elctrophoresis was used to test for cation-specific conformational changes in ribosomal RNA. Relative to magnesium-equilibrated RNA, barium ion and putrescine induced alterations in the electrophoretic behavior of ribosomal RNA while calcium ion produced no change. Exchange of a critical level of bound magnesium ion for barium or putrescine was necessary for these changes to take place. The alterations in electrophoretic behavior were unaffected by simply restoring magnesium ion, but in addition required heating for reversal. We suggest that these conformational changes are a result of interaction at a specific class of cation binding sites previously observed with intact ribosomes."}
{"id": "PMID:1094420", "title": "Sequence of E. coli tRNA-Glu1 by automated sequential degradation.", "content": "A minor tRNA-Glu1 constituent of a preparation of E. coli B tRNA-Glu (Oak Ridge) has a guanosine residue at position 66 rather than an adenosine as in the tRNA-Glu2 described by Ohashi et al. (3). Automated sequential degradation was used to sequence this region.", "contents": "Sequence of E. coli tRNA-Glu1 by automated sequential degradation. A minor tRNA-Glu1 constituent of a preparation of E. coli B tRNA-Glu (Oak Ridge) has a guanosine residue at position 66 rather than an adenosine as in the tRNA-Glu2 described by Ohashi et al. (3). Automated sequential degradation was used to sequence this region."}
{"id": "PMID:1094421", "title": "Modification of E. coli ribosomes and coliphage MS2 RNA by bisulfite: effects on ribosomal binding and protein synthesis.", "content": "The reaction of E. coli 70s ribosomes with 0.2 M NaH-35 s03 (pH 7.1, 3.5hrs, 37 degree) led to the conversion of 4.5% of the uracil residues of the R, RNA into 5.6-dihydrouracil-6-sulfonate residues. The modified ribosomes exhibited a significant decrease in their ability to bind (14-C)-phenylalanyl-(RNA-phe and to incorporate (14-C)-phenylalanine into protein in the presence of polyuridylic acid. The ability of the modified ribosomes to form an initiation complex as measured by the A-U-G or coliphage MS2 RNA dependent binding of (14-C)-fmet-tRNA-fmet was also impaired, as was their ability to incorporate (14-C) lysine into protein with MS2 RNA as messenger. Treatment os MS RNA with 0.2 M sodium (35-S) bisulfite, pH 7.0 at 25 degrees C resulted in the substitution of 2.7% and 6.2% of the uracil residues by bisulfite after 1 and 3.5 hrs of reaction, respectively. Impairment of function of the MS2 RNA in both initiation complex formation and transplantation assays was observed. These reactions of uracil residues of mRNA and rRNA may be a cause of biological damage inflicted by sodium bisulfite and sulfur dioxide.", "contents": "Modification of E. coli ribosomes and coliphage MS2 RNA by bisulfite: effects on ribosomal binding and protein synthesis. The reaction of E. coli 70s ribosomes with 0.2 M NaH-35 s03 (pH 7.1, 3.5hrs, 37 degree) led to the conversion of 4.5% of the uracil residues of the R, RNA into 5.6-dihydrouracil-6-sulfonate residues. The modified ribosomes exhibited a significant decrease in their ability to bind (14-C)-phenylalanyl-(RNA-phe and to incorporate (14-C)-phenylalanine into protein in the presence of polyuridylic acid. The ability of the modified ribosomes to form an initiation complex as measured by the A-U-G or coliphage MS2 RNA dependent binding of (14-C)-fmet-tRNA-fmet was also impaired, as was their ability to incorporate (14-C) lysine into protein with MS2 RNA as messenger. Treatment os MS RNA with 0.2 M sodium (35-S) bisulfite, pH 7.0 at 25 degrees C resulted in the substitution of 2.7% and 6.2% of the uracil residues by bisulfite after 1 and 3.5 hrs of reaction, respectively. Impairment of function of the MS2 RNA in both initiation complex formation and transplantation assays was observed. These reactions of uracil residues of mRNA and rRNA may be a cause of biological damage inflicted by sodium bisulfite and sulfur dioxide."}
{"id": "PMID:1094422", "title": "The activation of RNA polymerase by alkylammonium ions.", "content": "Tetramethylammonium and tetraethylammonium ions both lower the melting temperature of DNA. We have shown that these ions increase the activity of E. Coli RNA polymerase. When the base composition of the DNA template was varied, there was a correlation between the decrease in melting temperature and increase in RNA polymerase activity. No stimulation was observed when heat denatured DNA was used as template. It was shown that initiation of RNA synthesis was stimulated but to a smaller degree than was total RNA synthesis. The lag observed at low temperatures before RNA synthesis commences was found to be shorter in the presence of these alkyl ammonium ions. All these results are consistent with an unwinding of the double helix being an important step in transcription.", "contents": "The activation of RNA polymerase by alkylammonium ions. Tetramethylammonium and tetraethylammonium ions both lower the melting temperature of DNA. We have shown that these ions increase the activity of E. Coli RNA polymerase. When the base composition of the DNA template was varied, there was a correlation between the decrease in melting temperature and increase in RNA polymerase activity. No stimulation was observed when heat denatured DNA was used as template. It was shown that initiation of RNA synthesis was stimulated but to a smaller degree than was total RNA synthesis. The lag observed at low temperatures before RNA synthesis commences was found to be shorter in the presence of these alkyl ammonium ions. All these results are consistent with an unwinding of the double helix being an important step in transcription."}
{"id": "PMID:1094423", "title": "Interaction between phosphoribosyltransferase and purified histidine tRNA from wild type Salmonella typhimurium and a derepressed hisT mutant strain.", "content": "We have examined the interaction between phosphoribosyltransferase and purified tRNA-His from the wild type strain of Salmonella typhimurium, LT-2, and the histidine regulatory mutant hisTl504. Histidyl-tRNA from the mutant strain functions normally in protein synthesis but is defective in its role in the repression mechanism of the histidine operon. Phosphoribosyltransferase has been suggested as a possible aporegulator for this operon and as such might be expected to interact abnormally with tRNA-His from hisT1504. In these studies we have been unable to detect any difference between the affinities of phosphoribosyltransferase for tRNA-His from LT-2 or hisT1504, and thus we conclude that if the complex between phosphoribosyltransferase and histidyl-tRNA does function in regulation, the defect in the hisT1504 mutant must influence the interaction of the complex with some other regulatory element.", "contents": "Interaction between phosphoribosyltransferase and purified histidine tRNA from wild type Salmonella typhimurium and a derepressed hisT mutant strain. We have examined the interaction between phosphoribosyltransferase and purified tRNA-His from the wild type strain of Salmonella typhimurium, LT-2, and the histidine regulatory mutant hisTl504. Histidyl-tRNA from the mutant strain functions normally in protein synthesis but is defective in its role in the repression mechanism of the histidine operon. Phosphoribosyltransferase has been suggested as a possible aporegulator for this operon and as such might be expected to interact abnormally with tRNA-His from hisT1504. In these studies we have been unable to detect any difference between the affinities of phosphoribosyltransferase for tRNA-His from LT-2 or hisT1504, and thus we conclude that if the complex between phosphoribosyltransferase and histidyl-tRNA does function in regulation, the defect in the hisT1504 mutant must influence the interaction of the complex with some other regulatory element."}
{"id": "PMID:1094443", "title": "The avian inflammatory response: adaptation and utilization of skin windows.", "content": "A modified skin window system was developed and utilized for examination of the local inflammatory response in the chicken.  Data were collected at 2, 4, 6, 8, 12, 24, 28, 32 and 48 hours following wing-web scarification.  The first cells observed were heterophils and they remained the predominant type through 32 hours.  Mononuclears began infiltrating at a rapid rate of 8 to 12 hours, and by 48 hours they represented slightly more than one-half of the total cells observed.  The total number of cells present increased rapidly to a peak at 24 hours, and then declined during the 24 to 48 hour period.  The percentage of heterophils and mononuclears showed an inverse relationship throughout the 48 hour period, as appreciable numbers of eosinophils and basophils were not observed.  Macrophages were the predominant mononuclear cells.  Degranulation of heterophils was extensive in the 2 to 4 hour periods, but was much less common thereafter.  As the time after scarification increased, macrophages exhibited; (1) increased cytoplasm to nucleus ratio, (2) increased numbers of phagocytic vacuoles, and (3) a marked tendency to form giant cells.  The results obtained in this study were similar to those recorded for mammals, except that the percentage shift toward mononuclears occurred at a faster rate in mammals.", "contents": "The avian inflammatory response: adaptation and utilization of skin windows. A modified skin window system was developed and utilized for examination of the local inflammatory response in the chicken.  Data were collected at 2, 4, 6, 8, 12, 24, 28, 32 and 48 hours following wing-web scarification.  The first cells observed were heterophils and they remained the predominant type through 32 hours.  Mononuclears began infiltrating at a rapid rate of 8 to 12 hours, and by 48 hours they represented slightly more than one-half of the total cells observed.  The total number of cells present increased rapidly to a peak at 24 hours, and then declined during the 24 to 48 hour period.  The percentage of heterophils and mononuclears showed an inverse relationship throughout the 48 hour period, as appreciable numbers of eosinophils and basophils were not observed.  Macrophages were the predominant mononuclear cells.  Degranulation of heterophils was extensive in the 2 to 4 hour periods, but was much less common thereafter.  As the time after scarification increased, macrophages exhibited; (1) increased cytoplasm to nucleus ratio, (2) increased numbers of phagocytic vacuoles, and (3) a marked tendency to form giant cells.  The results obtained in this study were similar to those recorded for mammals, except that the percentage shift toward mononuclears occurred at a faster rate in mammals."}
{"id": "PMID:1094444", "title": "The purification of lymphocytes in chicken blood.", "content": "Several methods have been tried in attempts to purify the lymphocytes of chicken blood.  The most efficient one is based upon the properties of adhesion of the polymorphonuclears and monocytes.  With the use of a column filled with synthetic fibers, held at 37 degrees C., a high rate of lymphocyte purification was obtained.  Sedimentation and centrifugation then resulted in the elimination of almost all of the erythrocytes.  The final product was homogeneous and the lymphocytes obtained kept the physiological and metabolic properties necessary for their culture.", "contents": "The purification of lymphocytes in chicken blood. Several methods have been tried in attempts to purify the lymphocytes of chicken blood.  The most efficient one is based upon the properties of adhesion of the polymorphonuclears and monocytes.  With the use of a column filled with synthetic fibers, held at 37 degrees C., a high rate of lymphocyte purification was obtained.  Sedimentation and centrifugation then resulted in the elimination of almost all of the erythrocytes.  The final product was homogeneous and the lymphocytes obtained kept the physiological and metabolic properties necessary for their culture."}
{"id": "PMID:1094447", "title": "[Assessment of the somatotropic function of the hypophysis in patients with hypophyseal nanism and gonadal dysgenesis by using the insulin-tolerance and arginine-tolerance tests].", "content": "With the aid of insulin-tolerance and arginine tolerance tests (ITT and ATT, respectively) somatotropic function of the hypophysis was assessed in 18 patients with hypophyseal nanism and in 12--with gonad dysgenesis.  A marked delay in development and a premature exhaustion of the STH secretion was noted in the patients wiht hypophyseal nanism.  In patients with gonad dysgenesis the STH secretion was somewhat redeced during the ITT.  A change in the STH secretion was accompanied by a parallel depression of insulin secretion during the ATT.  Patients with hypophyseal nanism displayed a correlation between the extent of growth delay and the value of the STH secretion during the ITT (R=0.6, P smaller than 0.05).", "contents": "[Assessment of the somatotropic function of the hypophysis in patients with hypophyseal nanism and gonadal dysgenesis by using the insulin-tolerance and arginine-tolerance tests]. With the aid of insulin-tolerance and arginine tolerance tests (ITT and ATT, respectively) somatotropic function of the hypophysis was assessed in 18 patients with hypophyseal nanism and in 12--with gonad dysgenesis.  A marked delay in development and a premature exhaustion of the STH secretion was noted in the patients wiht hypophyseal nanism.  In patients with gonad dysgenesis the STH secretion was somewhat redeced during the ITT.  A change in the STH secretion was accompanied by a parallel depression of insulin secretion during the ATT.  Patients with hypophyseal nanism displayed a correlation between the extent of growth delay and the value of the STH secretion during the ITT (R=0.6, P smaller than 0.05)."}
{"id": "PMID:1094450", "title": "Endonucleolytic incision of x-irradiated deoxyribonucleic acid by extracts of Escherichia coli.", "content": "An enconuclease activity that reacts with x-irradiated DNA is present in extracts of E. coli. By using centrifugal methods to monitor the conversion of the supercoiled, circular double-stranded DNA for phage phi-x-174 (replicative form) or PM2 to the relaxed circular form it was possible to quantitate the rate of radiation induced endonuclease-sensitive sites in the DNA. For every single-strand break induced by x-rays under aerobic irradiation conditions, there is approximately one induced site sensitive to this endonuclease activity. Under irradiation conditions (addition OF Potassium iodide) that dramatically reduce rates of single-strand breaks and \"alkalilabile\" lesions, the number of endonuclease-sensitive sites relative to single-strand breaks increase approximatley 4-fold. This nuclease is present in several strains of E. coli B and K12, including mutants deficient in DNA polymerase I, recombination gene products (rec mutants), ultraviolet light incision enzyme (uvr A mutant), and endonuclease II. It is suggested that this endonuclease may be involved in an excision repair process for damages incurred in DNA by ionizing radiation.", "contents": "Endonucleolytic incision of x-irradiated deoxyribonucleic acid by extracts of Escherichia coli. An enconuclease activity that reacts with x-irradiated DNA is present in extracts of E. coli. By using centrifugal methods to monitor the conversion of the supercoiled, circular double-stranded DNA for phage phi-x-174 (replicative form) or PM2 to the relaxed circular form it was possible to quantitate the rate of radiation induced endonuclease-sensitive sites in the DNA. For every single-strand break induced by x-rays under aerobic irradiation conditions, there is approximately one induced site sensitive to this endonuclease activity. Under irradiation conditions (addition OF Potassium iodide) that dramatically reduce rates of single-strand breaks and \"alkalilabile\" lesions, the number of endonuclease-sensitive sites relative to single-strand breaks increase approximatley 4-fold. This nuclease is present in several strains of E. coli B and K12, including mutants deficient in DNA polymerase I, recombination gene products (rec mutants), ultraviolet light incision enzyme (uvr A mutant), and endonuclease II. It is suggested that this endonuclease may be involved in an excision repair process for damages incurred in DNA by ionizing radiation."}
{"id": "PMID:1094451", "title": "Tertiary hydrogen bonds in the solution structure of transfer RNA.", "content": "The high resolution nuclear magnetic resonance (NMR) spectra of hydrogen-bonded protons in four tRNAs have been studied at 270 MHz. The relative intensity of the resonances between -11 ppm and -15 ppm of Escherichia coli tRNA1-Va1 indicate that there are 26 plus or minus 3 protons, while only 20 are expected from secondary structure Watson-Crick hydrogen bonds inthe cloverleaf structure. Several possible candidates for these extra resonances are suggested by tertiary interactions observed in recent crystallographic studies. Of the four tRNAs studied, three, e.g., E. coli tRNA1Va1, E. coli tRNA-Arg and E. coli tRNA-Phe have one \"GU pair\" in their cloverleaf structure, while the fourth, yeast tRNA-Asp,has three \"GU pairs\" and one \"G pair\". Correlating these with the NMR spectra in the -10 ppm to -11 ppm region allows us to conclude that the \"GU pairs\" are not hydrogen-bonded by tautomerization to the lactim form. At the very low field region, near -14.9 ppm, the three E. coli tRNAs show a single resonance which is attributed to the 4-thiouracil 8 to adenine 14 hydrogen bond of the tertiary structure, by analogy with the recent crystal structure of yeast tRNA-Phe. This assignment is confirmed by the disappearance of this resonance after treatment with cyanogen bromide.", "contents": "Tertiary hydrogen bonds in the solution structure of transfer RNA. The high resolution nuclear magnetic resonance (NMR) spectra of hydrogen-bonded protons in four tRNAs have been studied at 270 MHz. The relative intensity of the resonances between -11 ppm and -15 ppm of Escherichia coli tRNA1-Va1 indicate that there are 26 plus or minus 3 protons, while only 20 are expected from secondary structure Watson-Crick hydrogen bonds inthe cloverleaf structure. Several possible candidates for these extra resonances are suggested by tertiary interactions observed in recent crystallographic studies. Of the four tRNAs studied, three, e.g., E. coli tRNA1Va1, E. coli tRNA-Arg and E. coli tRNA-Phe have one \"GU pair\" in their cloverleaf structure, while the fourth, yeast tRNA-Asp,has three \"GU pairs\" and one \"G pair\". Correlating these with the NMR spectra in the -10 ppm to -11 ppm region allows us to conclude that the \"GU pairs\" are not hydrogen-bonded by tautomerization to the lactim form. At the very low field region, near -14.9 ppm, the three E. coli tRNAs show a single resonance which is attributed to the 4-thiouracil 8 to adenine 14 hydrogen bond of the tertiary structure, by analogy with the recent crystal structure of yeast tRNA-Phe. This assignment is confirmed by the disappearance of this resonance after treatment with cyanogen bromide."}
{"id": "PMID:1094452", "title": "A link between streptomycin and rifampicin mutation.", "content": "Introduction of str A mutations frequently make \"male\" strains of Escherichia coli permissive to bacteriophage T7; certain rif mutations reverse the permissive effect of strA mutation. Permissiveness of the strA mutation is accompanied by enhanced transcription of bacteriophage T7 genome. Introduction of the nonpermissive rif allele to the permissive strA strain reduces or abolishes the transcription of T7 genome. Thus, a link is implied in the functioning of the ribosome and the RNA polymerase (RNA nucleotidyltransferase, EC 2.7.7.6).", "contents": "A link between streptomycin and rifampicin mutation. Introduction of str A mutations frequently make \"male\" strains of Escherichia coli permissive to bacteriophage T7; certain rif mutations reverse the permissive effect of strA mutation. Permissiveness of the strA mutation is accompanied by enhanced transcription of bacteriophage T7 genome. Introduction of the nonpermissive rif allele to the permissive strA strain reduces or abolishes the transcription of T7 genome. Thus, a link is implied in the functioning of the ribosome and the RNA polymerase (RNA nucleotidyltransferase, EC 2.7.7.6)."}
{"id": "PMID:1094453", "title": "DNA synthesis involving a complexes form of DNA polymerase I in extracts of Escherichia coli.", "content": "DNA polymerase I (EC 2.7.7.7; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase) has been recovered as a complex of about 390,000 molecular weight. The complex displays an ATP-stimulated DNA-synthesizing activity that prefers native to heat-denatured DNA. Genetic evidence indicates that the recBC enzyme is associated with the polymerase in the complex. Preliminary evidence for complexes involving DNA polymerases II and III is also presented.", "contents": "DNA synthesis involving a complexes form of DNA polymerase I in extracts of Escherichia coli. DNA polymerase I (EC 2.7.7.7; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase) has been recovered as a complex of about 390,000 molecular weight. The complex displays an ATP-stimulated DNA-synthesizing activity that prefers native to heat-denatured DNA. Genetic evidence indicates that the recBC enzyme is associated with the polymerase in the complex. Preliminary evidence for complexes involving DNA polymerases II and III is also presented."}
{"id": "PMID:1094454", "title": "On the significance of the retention of ligand by protein.", "content": "When a solution of binding protein and its ligand is dialyzed against a large volume of ligand-free medium the rate of exit of the ligand from the protein-containing compartment can be extremely slow, much slower than the rate observed in the absence of protein. This is what we call retention of ligand by protein. A simple calculation demonstrates that when the protein concentration is in large excess over the total ligand concentration, the exit of ligand follows quasi-first-order kinetics, the half-life being proportional to (1 + (P)/Kd), where (P) is the concentration of binding sites, and Kd the dissociation constant characteristic of the equilibrium between the ligand and the protein. Experimental verification of this relation is provided in the case of the periplasmic maltose-binding protein of Escherichia coli; The implications of the retention effect in biochemical techniques are discussed, as well as its possible significance in biological phenomena, such as bacterial chemotaxis and transport, mechanism of hormone action, or transmission of the nerve impulse.", "contents": "On the significance of the retention of ligand by protein. When a solution of binding protein and its ligand is dialyzed against a large volume of ligand-free medium the rate of exit of the ligand from the protein-containing compartment can be extremely slow, much slower than the rate observed in the absence of protein. This is what we call retention of ligand by protein. A simple calculation demonstrates that when the protein concentration is in large excess over the total ligand concentration, the exit of ligand follows quasi-first-order kinetics, the half-life being proportional to (1 + (P)/Kd), where (P) is the concentration of binding sites, and Kd the dissociation constant characteristic of the equilibrium between the ligand and the protein. Experimental verification of this relation is provided in the case of the periplasmic maltose-binding protein of Escherichia coli; The implications of the retention effect in biochemical techniques are discussed, as well as its possible significance in biological phenomena, such as bacterial chemotaxis and transport, mechanism of hormone action, or transmission of the nerve impulse."}
{"id": "PMID:1094455", "title": "Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.", "content": "A new group of mutants has been isolated which, during short pulses, incorporate (3-H)thymidine into DNA fragments that are substantially smaller than Okazaki fragments. These small fragments can be chased into DNA of high-molecular-weight, and thus may be precursors in DNA replication, During longer pulses, label also appears in DNA of higher molecular weight, although at an abnormally slow rate. The mutations map at a previously undescribed locus (dnaS) at 72 min on the E. coli chromosome.", "contents": "Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates. A new group of mutants has been isolated which, during short pulses, incorporate (3-H)thymidine into DNA fragments that are substantially smaller than Okazaki fragments. These small fragments can be chased into DNA of high-molecular-weight, and thus may be precursors in DNA replication, During longer pulses, label also appears in DNA of higher molecular weight, although at an abnormally slow rate. The mutations map at a previously undescribed locus (dnaS) at 72 min on the E. coli chromosome."}
{"id": "PMID:1094456", "title": "Diversity of light chain variable region sequences among rabbit antibodies elicited by the same antigens.", "content": "We report the complete variable region sequences of three homogeneous rabbit antibody light chains and the partial sequences of five others. Wehn these are compared to other published rabbit light chain sequences, two regions of markedly increased variability are revealed, which are homologous in position to the first and third hypervariable regions of murine and human myeloma light chains. In addition, there is increased variability among the first three residues at the aminoterminal end. A hypervariable region homologous to that identified at positions 50 to 56 in myeloma light chains is not present in these rabbit antibody light chains. The available three-dimensional models of Fab fragments based on x-ray crystallography indicate that neither the amino-terminal portion of the light chain nor the region homologous to positions 50 to 56 forms a part of the combining site. Comparison of the hypervariable regions among six light chains from antibodies to Type III pneumococcal polysaccharide and among four from antibodies to Type VIII pneumococcal polysaccharide suggests that a large number of different sequences may be found in antibodies specific for these relatively simple antigens. Certain residues outside of the hypervariable regions are invariant in the rabbit light chains and correspond to residues that are required for proper chain folding in human and murine myeloma light chains, indicating that the general conformation of myeloma light chains is the same as that of light chains of elicited antibodies.", "contents": "Diversity of light chain variable region sequences among rabbit antibodies elicited by the same antigens. We report the complete variable region sequences of three homogeneous rabbit antibody light chains and the partial sequences of five others. Wehn these are compared to other published rabbit light chain sequences, two regions of markedly increased variability are revealed, which are homologous in position to the first and third hypervariable regions of murine and human myeloma light chains. In addition, there is increased variability among the first three residues at the aminoterminal end. A hypervariable region homologous to that identified at positions 50 to 56 in myeloma light chains is not present in these rabbit antibody light chains. The available three-dimensional models of Fab fragments based on x-ray crystallography indicate that neither the amino-terminal portion of the light chain nor the region homologous to positions 50 to 56 forms a part of the combining site. Comparison of the hypervariable regions among six light chains from antibodies to Type III pneumococcal polysaccharide and among four from antibodies to Type VIII pneumococcal polysaccharide suggests that a large number of different sequences may be found in antibodies specific for these relatively simple antigens. Certain residues outside of the hypervariable regions are invariant in the rabbit light chains and correspond to residues that are required for proper chain folding in human and murine myeloma light chains, indicating that the general conformation of myeloma light chains is the same as that of light chains of elicited antibodies."}
{"id": "PMID:1094457", "title": "Excision of prophage lambda in a cell-free system.", "content": "A cell-free system that promotes the excision of prophage lambda DNA has been established. The substrate for the reaction is phage DNA carrying two attachment sites, which, in vivo, undergoes intramolecular recombination between these sites. The in vitro recombination system is efficient; 25-35% of the substrate DNA undergoes recombination in 30 min. There is an absolute requirement for ATP; Mg++ and spermidine are stimulatory. RNA does not appear to be involved, nor can a role for DNA synthesis be demonstrated.", "contents": "Excision of prophage lambda in a cell-free system. A cell-free system that promotes the excision of prophage lambda DNA has been established. The substrate for the reaction is phage DNA carrying two attachment sites, which, in vivo, undergoes intramolecular recombination between these sites. The in vitro recombination system is efficient; 25-35% of the substrate DNA undergoes recombination in 30 min. There is an absolute requirement for ATP; Mg++ and spermidine are stimulatory. RNA does not appear to be involved, nor can a role for DNA synthesis be demonstrated."}
{"id": "PMID:1094458", "title": "Mismatch repair in heteroduplex DNA.", "content": "DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one parental chain. (ii) The frequency of repair of a given mismatch to wild type depends on the marker, ranging from 3 to 20%. (iii) Excision tracts may extend several hundred nucleotides but are usually shorter than about 2000 nucleotides. (iv) In Rec-mediated bacteriophage crosses, recombination of markers closer than about 10-3 nucleotide pairs frequently occurs by mismatch repair within heteroduplex DNA. (V) The average amount of heteroduplex DNA formed in a Rec-mediated recombination event is a few thousand nucleotide pairs.", "contents": "Mismatch repair in heteroduplex DNA. DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one parental chain. (ii) The frequency of repair of a given mismatch to wild type depends on the marker, ranging from 3 to 20%. (iii) Excision tracts may extend several hundred nucleotides but are usually shorter than about 2000 nucleotides. (iv) In Rec-mediated bacteriophage crosses, recombination of markers closer than about 10-3 nucleotide pairs frequently occurs by mismatch repair within heteroduplex DNA. (V) The average amount of heteroduplex DNA formed in a Rec-mediated recombination event is a few thousand nucleotide pairs."}
{"id": "PMID:1094459", "title": "Permeability lesions in male Escherichia coli infected with bacteriophage T7.", "content": "The abortive development of bacteriophage T7 in E. coli cells carrying F factors has previously been attributed to a lack of virus-directed modification of ribosomes in such cells. We find it unnecessary to postulate such translational control to explain the failure of T7 development. Instead, there is a general cessation of macromolecular syntheses around 8 min after T7 infection of F' cells. This cessation is correlated with a sudden outflow of the entire acid-soluble pool of phosphorus-containing compounds and loss of the ability to accumulate amino acids. Manifestation of these defects requires expression of at least one T7 gene and one episomal gene.", "contents": "Permeability lesions in male Escherichia coli infected with bacteriophage T7. The abortive development of bacteriophage T7 in E. coli cells carrying F factors has previously been attributed to a lack of virus-directed modification of ribosomes in such cells. We find it unnecessary to postulate such translational control to explain the failure of T7 development. Instead, there is a general cessation of macromolecular syntheses around 8 min after T7 infection of F' cells. This cessation is correlated with a sudden outflow of the entire acid-soluble pool of phosphorus-containing compounds and loss of the ability to accumulate amino acids. Manifestation of these defects requires expression of at least one T7 gene and one episomal gene."}
{"id": "PMID:1094460", "title": "Cloning, isolation, and characterization of replication regions of complex plasmid genomes.", "content": "EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes.", "contents": "Cloning, isolation, and characterization of replication regions of complex plasmid genomes. EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes."}
{"id": "PMID:1094461", "title": "Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution.", "content": "The three-dimensional structure of the electron transport protein thioredoxin-S2 from E. coli has been determined from a 2.8 A resolution electron density map. The molecule is built up of a central core of three parallel and two antiparallel strands of pleated sheet surrounded by four helices. Thr residues involved in the active center 14-membered disulfide ring of thioredoxin form a protrusion between one of the helices and the middle strand of the pleated sheet. This region of the molecule, comprising two parallel strands joined by the protrusion and a helix, is structurally very similar to corresponding functionally important regions in the nucleotide-binding domains of flavodoxin and the dehydrogenases. The molecule has about 75% of the residues in well-defined secondary structures. The structure indicates that the carboxy-terminal third of the molecule forms an independent folding unit consisting of two strands of antiparallel pleated sheet and a terminal alpha-helix. This agress with the noncovalent reconstitution experiments from thioredoxin peptide fragments. Thioredoxin is an example of a protein with the active center located on a protrusion rather than in a cleft, thus demonstrating the existence of male proteins.", "contents": "Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution. The three-dimensional structure of the electron transport protein thioredoxin-S2 from E. coli has been determined from a 2.8 A resolution electron density map. The molecule is built up of a central core of three parallel and two antiparallel strands of pleated sheet surrounded by four helices. Thr residues involved in the active center 14-membered disulfide ring of thioredoxin form a protrusion between one of the helices and the middle strand of the pleated sheet. This region of the molecule, comprising two parallel strands joined by the protrusion and a helix, is structurally very similar to corresponding functionally important regions in the nucleotide-binding domains of flavodoxin and the dehydrogenases. The molecule has about 75% of the residues in well-defined secondary structures. The structure indicates that the carboxy-terminal third of the molecule forms an independent folding unit consisting of two strands of antiparallel pleated sheet and a terminal alpha-helix. This agress with the noncovalent reconstitution experiments from thioredoxin peptide fragments. Thioredoxin is an example of a protein with the active center located on a protrusion rather than in a cleft, thus demonstrating the existence of male proteins."}
{"id": "PMID:1094462", "title": "Ribosomal protein S1 and polypeptide chain initiation in bacteria.", "content": "Among several subspecies of 30S subunits of Escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein S1-containing subspecies participates in the formation of the 30S initiation complex with coliphage MS2 RNA as mRNA; the other subspecies retain activity with AUG as mRNA; they are also active in the poly(U)-directed binding of Phe-tRNA. Protein S1 from Caulobacter crescentus substitutes for E. coli S1 despite the fact that C. crescentus ribosomes do not bind MS2 RNA. Under appropriate conditions, the entire population of E. coli 30S subunits can be isolated as the S1-containing subspecies. Protein S1 is lost by salt treatment of ribosomes.", "contents": "Ribosomal protein S1 and polypeptide chain initiation in bacteria. Among several subspecies of 30S subunits of Escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein S1-containing subspecies participates in the formation of the 30S initiation complex with coliphage MS2 RNA as mRNA; the other subspecies retain activity with AUG as mRNA; they are also active in the poly(U)-directed binding of Phe-tRNA. Protein S1 from Caulobacter crescentus substitutes for E. coli S1 despite the fact that C. crescentus ribosomes do not bind MS2 RNA. Under appropriate conditions, the entire population of E. coli 30S subunits can be isolated as the S1-containing subspecies. Protein S1 is lost by salt treatment of ribosomes."}
{"id": "PMID:1094463", "title": "Model for regulation of Escherichia coli DNA repair functions.", "content": "A feedback loop for the regulation of the rec/lex-mediated DNA repair system is proposed. This model was formulated from experiments on the genetic and metabolic regulation of the rate of synthesis of protein X performed in this laboratory, and from genetic data obtained in other laboratories. Protein X is proposed to prevent DNA degradation by the recBC-coded exonuclease. The model states tht: (1) The lex (or exrA in E. coli B) gene codes for a repressor. (2) This repressor binds to an operator region of DNA consisting of the tif-zab region at 51 minutes on the E. coli chromosome. (3) The operator region controls the production of several proteins involved in DNA repair, including protein X. (4) The recA gene product is required to remove the lex-coded repressor from the operator. Thre recA gene could code for an antirepressor (inducer protein or a protease) or a modifer of recBC nuclease action; (5) Low molecular weight products of DNA degradation are effectors that activate the system. (6) Protein X limits recBC nuclease action by binding to single-stranded DNA.", "contents": "Model for regulation of Escherichia coli DNA repair functions. A feedback loop for the regulation of the rec/lex-mediated DNA repair system is proposed. This model was formulated from experiments on the genetic and metabolic regulation of the rate of synthesis of protein X performed in this laboratory, and from genetic data obtained in other laboratories. Protein X is proposed to prevent DNA degradation by the recBC-coded exonuclease. The model states tht: (1) The lex (or exrA in E. coli B) gene codes for a repressor. (2) This repressor binds to an operator region of DNA consisting of the tif-zab region at 51 minutes on the E. coli chromosome. (3) The operator region controls the production of several proteins involved in DNA repair, including protein X. (4) The recA gene product is required to remove the lex-coded repressor from the operator. Thre recA gene could code for an antirepressor (inducer protein or a protease) or a modifer of recBC nuclease action; (5) Low molecular weight products of DNA degradation are effectors that activate the system. (6) Protein X limits recBC nuclease action by binding to single-stranded DNA."}
{"id": "PMID:1094464", "title": "Poly(adenylic acid) sequences in the RNA of Caulobacter crescenus.", "content": "Poly(adenylic acid) sequences have been isolated from the Gram-negative bacterium Caulobacter crescentus. Most of these A-rich tracts are associated with large RNA molecules that constitute an important fraction of the unstable RNA in these bacteria, and, as estimated by poly(U) filter binding, they are not present in the 16S or 23S ribosomal RNA. Preliminary estimates of size from polyacrylamide gel electrophoresis suggest that the majority of the A-rich tracts ranges from 15 to approximately 50 residues in length.", "contents": "Poly(adenylic acid) sequences in the RNA of Caulobacter crescenus. Poly(adenylic acid) sequences have been isolated from the Gram-negative bacterium Caulobacter crescentus. Most of these A-rich tracts are associated with large RNA molecules that constitute an important fraction of the unstable RNA in these bacteria, and, as estimated by poly(U) filter binding, they are not present in the 16S or 23S ribosomal RNA. Preliminary estimates of size from polyacrylamide gel electrophoresis suggest that the majority of the A-rich tracts ranges from 15 to approximately 50 residues in length."}
{"id": "PMID:1094465", "title": "Immunosuppressive effect of syngeneic thymus cells on allograft rejection.", "content": "Transfer of thymus cells from young chickens to syngeneic recipients suppresses the allograft rejection between strains differing at the major histocompatibility (B) locus. Thymus cell transfer in combination with a light whole body irradiation (360 R) prolongs significantly the mean rejection time of skin allografts and leads in a proportion of recipients to long-lasting graft survival (greater than 200 days). Three weeks after the cell transfer, the suppression appears to be antigen specific, as judged by the normal reactivity against third-party skin grafts. From the types of thymus cells preparations that are effective in these experiments, it is inferred that the suppressor cell is a bursa-dependent lymphocyte, which is predominantly found in the young chicken thymus and which is different from B-lymphocytes, B-precursor cells, or graft-versus-host-reactive T-cells.", "contents": "Immunosuppressive effect of syngeneic thymus cells on allograft rejection. Transfer of thymus cells from young chickens to syngeneic recipients suppresses the allograft rejection between strains differing at the major histocompatibility (B) locus. Thymus cell transfer in combination with a light whole body irradiation (360 R) prolongs significantly the mean rejection time of skin allografts and leads in a proportion of recipients to long-lasting graft survival (greater than 200 days). Three weeks after the cell transfer, the suppression appears to be antigen specific, as judged by the normal reactivity against third-party skin grafts. From the types of thymus cells preparations that are effective in these experiments, it is inferred that the suppressor cell is a bursa-dependent lymphocyte, which is predominantly found in the young chicken thymus and which is different from B-lymphocytes, B-precursor cells, or graft-versus-host-reactive T-cells."}
{"id": "PMID:1094466", "title": "Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation.", "content": "A temperature-sensitive mutant having a lethal mutation in the gene for the beta subunit of RNA polymerase (nucleosidetriphosphate:RNA  nucleotidyltransferase, EC 2.7.7.6) exhibits an apparent 2- to 3-fold decrease in the rates of both beta and beta' subunit synthesis at the non-permissive temperature, relative to total protein. In contrast, a temperature-sensitive mutant with a lethal mutation in the gene encoding beta' has a 5- to 6-fold increase in the rates of beta and beta' synthesis at 42 degrees. These beta and beta' mutants also exhibit rapid degradation of both subunits at the high temperature.", "contents": "Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation. A temperature-sensitive mutant having a lethal mutation in the gene for the beta subunit of RNA polymerase (nucleosidetriphosphate:RNA  nucleotidyltransferase, EC 2.7.7.6) exhibits an apparent 2- to 3-fold decrease in the rates of both beta and beta' subunit synthesis at the non-permissive temperature, relative to total protein. In contrast, a temperature-sensitive mutant with a lethal mutation in the gene encoding beta' has a 5- to 6-fold increase in the rates of beta and beta' synthesis at 42 degrees. These beta and beta' mutants also exhibit rapid degradation of both subunits at the high temperature."}
{"id": "PMID:1094467", "title": "Uptake of homologous single-stranded fragments by superhelical DNA: a possible mechanism for initiation of genetic recombination.", "content": "Superhelical [3-H]DNA (replicative form I, RFI) of bacteriophage phiX174 slowly but spontaneously took up 32-P-labeled homologous single-stranded fragments at 4 degrees. Uptake was accelerated by heating to 75 degrees. RFI did not take up single-stranded fragments derived from DNA of Escherichia coli or from separated strands of phage lambda. Uptake was inhibited by low concentrations of ethidium bromide. Relaxed circular phiX174 DNA did not take up homologous fragments. Per molecule of RFI, the complexes contained as much as 90 nucleotide residues of homologous fragment. The 32-P-lebeled fragments were largely resistant to digestion by exonuclease I, and were not displaced by heating complexes at 60 degrees for 1 min in 16 mM or 100 mM NaCl. Under comparable conditions of temperature and salt all of the fragments were displaced from complexes in which at least one phosphodiester bond was cleaved by pancreatic DNase, but a significant fraction of the fragments was retained in complexes that were relaxed by digestion with S1 nuclease. These observations are interpreted to mean that S1 nuclease digested the plus (viral) strand of the recipient RF at the site of uptake in some instances. Transfection of E. coli by heterozygous complexes produced recombinant progeny, thereby showing that genetic information can be transferred from the fragment of plus strand to progeny plus strands. We propose that both uptake of a third strand by superhelical DNA and the action of nucleases on the resulting complex may simulate early steps in genetic recombination.", "contents": "Uptake of homologous single-stranded fragments by superhelical DNA: a possible mechanism for initiation of genetic recombination. Superhelical [3-H]DNA (replicative form I, RFI) of bacteriophage phiX174 slowly but spontaneously took up 32-P-labeled homologous single-stranded fragments at 4 degrees. Uptake was accelerated by heating to 75 degrees. RFI did not take up single-stranded fragments derived from DNA of Escherichia coli or from separated strands of phage lambda. Uptake was inhibited by low concentrations of ethidium bromide. Relaxed circular phiX174 DNA did not take up homologous fragments. Per molecule of RFI, the complexes contained as much as 90 nucleotide residues of homologous fragment. The 32-P-lebeled fragments were largely resistant to digestion by exonuclease I, and were not displaced by heating complexes at 60 degrees for 1 min in 16 mM or 100 mM NaCl. Under comparable conditions of temperature and salt all of the fragments were displaced from complexes in which at least one phosphodiester bond was cleaved by pancreatic DNase, but a significant fraction of the fragments was retained in complexes that were relaxed by digestion with S1 nuclease. These observations are interpreted to mean that S1 nuclease digested the plus (viral) strand of the recipient RF at the site of uptake in some instances. Transfection of E. coli by heterozygous complexes produced recombinant progeny, thereby showing that genetic information can be transferred from the fragment of plus strand to progeny plus strands. We propose that both uptake of a third strand by superhelical DNA and the action of nucleases on the resulting complex may simulate early steps in genetic recombination."}
{"id": "PMID:1094468", "title": "An intercistronic region and ribosome-binding site in bacterial messenger RNA.", "content": "A messenger RNA fragment about 220 nucleotides long has been isolated from 32-P-labeled tryptophan operon mRNA of Escherichia coli. When point mutations at the end of trpB and the beginning of trpA were introduced, the resulting nucleotide changes were found; hence the mRNA fragment must include the trpB-trpA intercistronic region. Most of the nucleotide sequences can be assigned to specific locations in the structural genes, based on the amino-acid sequences of the trpB and trpA proteins. In vitro, ribosomes bind to this piece of mRNA and protect from nuclease attack a region about 40 nucleotides long, containing a central AUG codon. The triplet codons to the 3' side of this AUG correspond to the first seven amino acids of the trpA protein; the codons to the 5' side correspond to the last six amino acids of the trpB protein. Translation of trpB is terminated by single UGA codon, which overlaps the trpA AUG initiation codon: UGAUG. Thus the untranslated \"intercistronic\" region consists of only two nucleotides. The RNA sequence spanning this region undoubtedly fulfills two functions, specifying ribosome recognition signals as well as encoding amino-acid sequences.", "contents": "An intercistronic region and ribosome-binding site in bacterial messenger RNA. A messenger RNA fragment about 220 nucleotides long has been isolated from 32-P-labeled tryptophan operon mRNA of Escherichia coli. When point mutations at the end of trpB and the beginning of trpA were introduced, the resulting nucleotide changes were found; hence the mRNA fragment must include the trpB-trpA intercistronic region. Most of the nucleotide sequences can be assigned to specific locations in the structural genes, based on the amino-acid sequences of the trpB and trpA proteins. In vitro, ribosomes bind to this piece of mRNA and protect from nuclease attack a region about 40 nucleotides long, containing a central AUG codon. The triplet codons to the 3' side of this AUG correspond to the first seven amino acids of the trpA protein; the codons to the 5' side correspond to the last six amino acids of the trpB protein. Translation of trpB is terminated by single UGA codon, which overlaps the trpA AUG initiation codon: UGAUG. Thus the untranslated \"intercistronic\" region consists of only two nucleotides. The RNA sequence spanning this region undoubtedly fulfills two functions, specifying ribosome recognition signals as well as encoding amino-acid sequences."}
{"id": "PMID:1094469", "title": "Hair dyes are mutagenic: identification of a variety of mutagenic ingredients.", "content": "We have previously described a sensitive bacterial test for dectecting carcinogens as mutagens. We have previously described a sensitive bacterial test for detecting carcinogens as mutagens. We show here that 89% (150/169) of commercial oxidative-type (hydrogen peroxide) hair dye formulations are mutagenic in this test. Of the 18 components of these hair dyes, nine show various degrees of mutagenicity:2,4-diaminoanisole, 4-nitro-o-phenylenediamine,  2-nitro-p-phenylenediamine, 2,5-diaminoanisole, 2-amino-5-nitrophenol, m-phenylenediamine,  o-phenylenediamine,  2-amino-4-nitrophenol, and 2,5-diaminotoluene. Three hair dye components (p-phenylenediamine, 2,5-diaminotuluene, and 2,5-diaminoanisole) become strongly mutagenic after oxidation by H2O2: the mutagenic product of p-phenylenediamine is identified as the known trimer, Bandrowski's base. 2,4-Diaminotoluene, a hair dye component until recently, is also shown to be mutagenic: this compound has been shown to be a carcinogen in rats and is used in large amounts in the polyurethane foam industry. About 20,000,000 people (mostly women) dye their hair in the U.S. and the hazard could be considerable if these chemicals are actually mutagenic and carcinogenic in humans.", "contents": "Hair dyes are mutagenic: identification of a variety of mutagenic ingredients. We have previously described a sensitive bacterial test for dectecting carcinogens as mutagens. We have previously described a sensitive bacterial test for detecting carcinogens as mutagens. We show here that 89% (150/169) of commercial oxidative-type (hydrogen peroxide) hair dye formulations are mutagenic in this test. Of the 18 components of these hair dyes, nine show various degrees of mutagenicity:2,4-diaminoanisole, 4-nitro-o-phenylenediamine,  2-nitro-p-phenylenediamine, 2,5-diaminoanisole, 2-amino-5-nitrophenol, m-phenylenediamine,  o-phenylenediamine,  2-amino-4-nitrophenol, and 2,5-diaminotoluene. Three hair dye components (p-phenylenediamine, 2,5-diaminotuluene, and 2,5-diaminoanisole) become strongly mutagenic after oxidation by H2O2: the mutagenic product of p-phenylenediamine is identified as the known trimer, Bandrowski's base. 2,4-Diaminotoluene, a hair dye component until recently, is also shown to be mutagenic: this compound has been shown to be a carcinogen in rats and is used in large amounts in the polyurethane foam industry. About 20,000,000 people (mostly women) dye their hair in the U.S. and the hazard could be considerable if these chemicals are actually mutagenic and carcinogenic in humans."}
{"id": "PMID:1094470", "title": "Effect of growth conditions on DNA-membrane attachment in Escherichia coli.", "content": "DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis, (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of replication points is most consistent with attachment of all replication points and attachment of at least one other chromosomal site; it is inconsistent with attachment of the replication points only, the chromosomal origin only, or the chromosomal terminus only. (b) When repair DNA synthesis is stimulated by irradiation with ultraviolet light, the DNA-membrane attachment remains unchanged.", "contents": "Effect of growth conditions on DNA-membrane attachment in Escherichia coli. DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis, (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of replication points is most consistent with attachment of all replication points and attachment of at least one other chromosomal site; it is inconsistent with attachment of the replication points only, the chromosomal origin only, or the chromosomal terminus only. (b) When repair DNA synthesis is stimulated by irradiation with ultraviolet light, the DNA-membrane attachment remains unchanged."}
{"id": "PMID:1094471", "title": "Presence of actin during chromosomal movement.", "content": "Actin has been shown to be present in the nucleoli, kinetochore and centriolar regions, and in the mitotic spindle of rat kangaroo cells which have been stained with fluorescently labeled heavy meromyosin. The actin in the spindle is confined to the fibers that connect the chromosomes with the centriolar region. Actin was not present in astral fibers, in the continuous spindle fibers that connect the poles, or in non-kinetochore regions of the chromosomes. The specific localization of actin in chromosomal spindle fibers suggests an actin-mvosin interaction as the force-producing mechanism for chromosomal movement.", "contents": "Presence of actin during chromosomal movement. Actin has been shown to be present in the nucleoli, kinetochore and centriolar regions, and in the mitotic spindle of rat kangaroo cells which have been stained with fluorescently labeled heavy meromyosin. The actin in the spindle is confined to the fibers that connect the chromosomes with the centriolar region. Actin was not present in astral fibers, in the continuous spindle fibers that connect the poles, or in non-kinetochore regions of the chromosomes. The specific localization of actin in chromosomal spindle fibers suggests an actin-mvosin interaction as the force-producing mechanism for chromosomal movement."}
{"id": "PMID:1094472", "title": "Daily periodicity in the activity of a slide used to perform immunologic reactions at a liquid-solid interface.", "content": "Nickel-plated slides were prepared by evaporating a nickel layer (congruent to 4000 A thick) on glass slides in the presence of a magnetic field whose lines of force were perpendicular to the surface of the slides. Such slides are called active. After being coated with a layer of bovine albumin, they could absorb a layer of antibodies 70-80 A thick. However, if the active slides before they were coated with bovine serum albumin, were submitted to a magnetic field with lines of force parallel to the surface, the layer of antibodies absorbed was only 40 A thick. They had become inactive. It has been found that slides remain active at night but that shortly after sunrise they become slowly inactivated and reach a minimum in their activity at exactly the midday period. They regain full activity at sunset. It is shown that the inactivation results from a solar radiation that can be stopped by 3.5 cm of lead. On December 13th, 1974 there was an eclipse of the sun with 65% occultation at noon (Daylight Saving Time). The activity of the slide at noon was 65% of the maximum activity (83 A) observed before sunrise. The thickness of the adsorbed layer of antibodies were 75 A instead of 63 A observed in the absence of the eclipse. The activation of the slides originates in a radiation of non-solar origin that is adsorbed by 1 can of lead.", "contents": "Daily periodicity in the activity of a slide used to perform immunologic reactions at a liquid-solid interface. Nickel-plated slides were prepared by evaporating a nickel layer (congruent to 4000 A thick) on glass slides in the presence of a magnetic field whose lines of force were perpendicular to the surface of the slides. Such slides are called active. After being coated with a layer of bovine albumin, they could absorb a layer of antibodies 70-80 A thick. However, if the active slides before they were coated with bovine serum albumin, were submitted to a magnetic field with lines of force parallel to the surface, the layer of antibodies absorbed was only 40 A thick. They had become inactive. It has been found that slides remain active at night but that shortly after sunrise they become slowly inactivated and reach a minimum in their activity at exactly the midday period. They regain full activity at sunset. It is shown that the inactivation results from a solar radiation that can be stopped by 3.5 cm of lead. On December 13th, 1974 there was an eclipse of the sun with 65% occultation at noon (Daylight Saving Time). The activity of the slide at noon was 65% of the maximum activity (83 A) observed before sunrise. The thickness of the adsorbed layer of antibodies were 75 A instead of 63 A observed in the absence of the eclipse. The activation of the slides originates in a radiation of non-solar origin that is adsorbed by 1 can of lead."}
{"id": "PMID:1094487", "title": "Cystography after kidney transplantation.", "content": "45 voiding cystographies were performed in 40 patients with renal allografts: in 30 cases diluted Verografin SPOFA (CSSR) and in 15 cases Opacist E.R. BRACCO (Italy) were used as contrast medium. The purpose of the study was to assess the frequency of vesico-ureteral reflux into the autologous ureters, into the ureter and pelvicalyceal system of the graft and the frequency of morphological and functional changes of the urinary bladder including its contraction during micturition. In three patients with urinary leakage at the site of uretero-neocystotomy voiding cystography gave false-negative results.", "contents": "Cystography after kidney transplantation. 45 voiding cystographies were performed in 40 patients with renal allografts: in 30 cases diluted Verografin SPOFA (CSSR) and in 15 cases Opacist E.R. BRACCO (Italy) were used as contrast medium. The purpose of the study was to assess the frequency of vesico-ureteral reflux into the autologous ureters, into the ureter and pelvicalyceal system of the graft and the frequency of morphological and functional changes of the urinary bladder including its contraction during micturition. In three patients with urinary leakage at the site of uretero-neocystotomy voiding cystography gave false-negative results."}
{"id": "PMID:1094483", "title": "A review of the cardiovascular effects and toxicity of tricyclic antidepressants.", "content": "The tricyclic antidepressants have potent and complex effects on the cardiovascular system, both directly and through interactions with other drugs. Through a combination of anticholinergic activity, direct myocardial depressant activity and an effect on the adrenergic neuron, they can cause a combination of arrhythmias, blood pressure abnormalities and congestive heart failure. These changes, although most common with overdose, can occur at therapeutic levels. In addition a number of drug-drug interactions can occur between the tricyclics and agents used in the treatment of cardiovascular disease, as exemplified by tricyclic antagonism of the antihypertensive effect of guanethidine. Awareness of these factors is necessary to insure proper therapeutic utilization of the tricyclics as well as to treat effectively their toxicity.", "contents": "A review of the cardiovascular effects and toxicity of tricyclic antidepressants. The tricyclic antidepressants have potent and complex effects on the cardiovascular system, both directly and through interactions with other drugs. Through a combination of anticholinergic activity, direct myocardial depressant activity and an effect on the adrenergic neuron, they can cause a combination of arrhythmias, blood pressure abnormalities and congestive heart failure. These changes, although most common with overdose, can occur at therapeutic levels. In addition a number of drug-drug interactions can occur between the tricyclics and agents used in the treatment of cardiovascular disease, as exemplified by tricyclic antagonism of the antihypertensive effect of guanethidine. Awareness of these factors is necessary to insure proper therapeutic utilization of the tricyclics as well as to treat effectively their toxicity."}
{"id": "PMID:1094488", "title": "Further evaluation of 99m-Tc sulfur colloid accumulation in rejecting renal transplants in man and a canine model.", "content": "Renal transplant accumulation of 99m-Tc sulfur colloid was evaluated in 41 patients. Ninety-five examinations were performed comparing transplant with bone marrow activity and clinical or pathological diagnosis. 99m-Tc sulfur colloid accumulated in acute or chronic rejection as long as the vascular supply of the grafted kidney was not severely impaired. Accumulation was marked in chronic rejection, slight in acute rejection and absent in normally functioning transplants or in those with acute tubular necrosis. In the 10 dog models, no accumulation was seen in autografted transplants and early accumulation was demonstrated within the hyperacutely rejecting kidneys of allografted dogs.", "contents": "Further evaluation of 99m-Tc sulfur colloid accumulation in rejecting renal transplants in man and a canine model. Renal transplant accumulation of 99m-Tc sulfur colloid was evaluated in 41 patients. Ninety-five examinations were performed comparing transplant with bone marrow activity and clinical or pathological diagnosis. 99m-Tc sulfur colloid accumulated in acute or chronic rejection as long as the vascular supply of the grafted kidney was not severely impaired. Accumulation was marked in chronic rejection, slight in acute rejection and absent in normally functioning transplants or in those with acute tubular necrosis. In the 10 dog models, no accumulation was seen in autografted transplants and early accumulation was demonstrated within the hyperacutely rejecting kidneys of allografted dogs."}
{"id": "PMID:1094489", "title": "Multiple chronic traumatic pseudoaneurysms of the aorta and great vessels. A case report.", "content": "A case of multiple chronic traumatic pseudoaneurysms of the thoracic aorta and major branches in a young girl is presented. Angiograms obtained 2 days, 3 months, and 15 months after injury demonstrated stability of the patients's condition. The necessity for close follow-up in a case not surgically repaired is stressed.", "contents": "Multiple chronic traumatic pseudoaneurysms of the aorta and great vessels. A case report. A case of multiple chronic traumatic pseudoaneurysms of the thoracic aorta and major branches in a young girl is presented. Angiograms obtained 2 days, 3 months, and 15 months after injury demonstrated stability of the patients's condition. The necessity for close follow-up in a case not surgically repaired is stressed."}
{"id": "PMID:1094493", "title": "Cholinesterase in embryonic development.", "content": "I. Cholinesterase (ChE) activity was studied histochemically during early development of the sea urchin, the amphibian, the chick and the rat embryo. After formalin fixation and embedding in water-soluble carbowax, the enzyme reaction was carried out in serial section. 2. Independent from innervation ChE appears in every embryonic blastema in a very early stage of development. It disappears from the embryonic cells after they have assembled into definite organ structures. Thus, ChE plays a role in embryonic development which is different from its known function in the adult. Therefore, ChE activity present in differentiating cells during a limited phase of development, is termed \"embryonic cholinesterase\". 3. Embryonic ChE was invariably found in cells engaged in morphogenetic movements. This observation has led us to suppose that the enzyme in involved in the regulation of cellular movements during development. 4. In particular, embryonic ChE is described in the following locations: a) During sea urchin gastrulation ChE is present in the primary mesenchymal cells emigrating from the blastula wall and in the archenteron cells which are known to bring about the invagination movement by contraction of their pseudopods. b) In the early chick blastoderm ChE active \"droplet cells\" are described which are supposed to emigrate from the epiblast layer in order to form the hypoblast. c) During development of notochord and somites, during closure of the neural tube and development of the head anlage, the close correlation of ChE activity with various morphogenetic movements is demonstrated: ChE appears during aggregation and desaggregation of epithelial compounds. The active bending of preexisting epithelial sheets, such as the neural plate, is also accompanied by ChE activity in epithelial cells...", "contents": "Cholinesterase in embryonic development. I. Cholinesterase (ChE) activity was studied histochemically during early development of the sea urchin, the amphibian, the chick and the rat embryo. After formalin fixation and embedding in water-soluble carbowax, the enzyme reaction was carried out in serial section. 2. Independent from innervation ChE appears in every embryonic blastema in a very early stage of development. It disappears from the embryonic cells after they have assembled into definite organ structures. Thus, ChE plays a role in embryonic development which is different from its known function in the adult. Therefore, ChE activity present in differentiating cells during a limited phase of development, is termed \"embryonic cholinesterase\". 3. Embryonic ChE was invariably found in cells engaged in morphogenetic movements. This observation has led us to suppose that the enzyme in involved in the regulation of cellular movements during development. 4. In particular, embryonic ChE is described in the following locations: a) During sea urchin gastrulation ChE is present in the primary mesenchymal cells emigrating from the blastula wall and in the archenteron cells which are known to bring about the invagination movement by contraction of their pseudopods. b) In the early chick blastoderm ChE active \"droplet cells\" are described which are supposed to emigrate from the epiblast layer in order to form the hypoblast. c) During development of notochord and somites, during closure of the neural tube and development of the head anlage, the close correlation of ChE activity with various morphogenetic movements is demonstrated: ChE appears during aggregation and desaggregation of epithelial compounds. The active bending of preexisting epithelial sheets, such as the neural plate, is also accompanied by ChE activity in epithelial cells..."}
{"id": "PMID:1094516", "title": "Early morphological alterations of the rat lung with increased intracranial pressure. II. A scanning electron microscopic study using different fixation procedures.", "content": "Pulmonary edema following intracranial pressure elevation was studied by means of scanning electron microscopy. Using three different fixation procedures-immersion, vascular perfusion, and tracheal instillation-the various features of intraalveolar edema formation could be demonstrated: capillary congestion, intraalveolar proteinious exudate, and capillary hemorrhage.", "contents": "Early morphological alterations of the rat lung with increased intracranial pressure. II. A scanning electron microscopic study using different fixation procedures. Pulmonary edema following intracranial pressure elevation was studied by means of scanning electron microscopy. Using three different fixation procedures-immersion, vascular perfusion, and tracheal instillation-the various features of intraalveolar edema formation could be demonstrated: capillary congestion, intraalveolar proteinious exudate, and capillary hemorrhage."}
{"id": "PMID:1094517", "title": "[The effect of acetyl-salicylic-acid and a pyrido-pyrimidine-derivate on the rejection of allotransplanted canine kidneys (author's transl)].", "content": "The effects of anti-thrombotic drugs-Acetyl-salicylic-acid and 2-Piperazinly-4-thiomorpholino-pyrido(3,2-d)-pyrimidin-sulfat-trihydrat-on rejection reactions of renal allografts in dogs have been studied. From the clinical point of view no effectiveness of the two drugs can be found, whereas the histological findings do clearly show diminished rejection reactions in the Acetylsalicylic-acid group.", "contents": "[The effect of acetyl-salicylic-acid and a pyrido-pyrimidine-derivate on the rejection of allotransplanted canine kidneys (author's transl)]. The effects of anti-thrombotic drugs-Acetyl-salicylic-acid and 2-Piperazinly-4-thiomorpholino-pyrido(3,2-d)-pyrimidin-sulfat-trihydrat-on rejection reactions of renal allografts in dogs have been studied. From the clinical point of view no effectiveness of the two drugs can be found, whereas the histological findings do clearly show diminished rejection reactions in the Acetylsalicylic-acid group."}
{"id": "PMID:1094528", "title": "Effect of listerine on dental plaque and gingivitis.", "content": "The present study was performed in 10 adults in order to evaluate the effect of an antiseptic mouthrinse (Listerine) on the rate of dental plaque formation and gingivitis development during a 2-week period when all efforts towards active mechanical oral hygiene were withdrawn. The study was performed as a crossover study and was carried out during four consecutive 2-week periods. During the first and third periods (preparatory periods) the participants were subjected to repeated professional tooth cleanings in order to establish plaque- and gingivitis-free dentitions. During the second and fourth periods (test and control periods) the participants were not allowed to brush their teeth but rinsed their mouths three times a day with Listerine or a placebo mouthwash. Plaque Index, Gingival Index, gingival fluid flow, and crevicular leukocytes were assessed on d 0, 2, 4, 7, and 14. On d 7 and 14, dental plaque was removed from the right and left jaws respectively and the wet weights determined. The chemotactic activity elaborated by the plaques was studied in Boyden chambers. During the Listerine test period, significantly lower Plaque and Gingival Index values were scored and lower amounts of plaque could be sampled in comparison to the control period.", "contents": "Effect of listerine on dental plaque and gingivitis. The present study was performed in 10 adults in order to evaluate the effect of an antiseptic mouthrinse (Listerine) on the rate of dental plaque formation and gingivitis development during a 2-week period when all efforts towards active mechanical oral hygiene were withdrawn. The study was performed as a crossover study and was carried out during four consecutive 2-week periods. During the first and third periods (preparatory periods) the participants were subjected to repeated professional tooth cleanings in order to establish plaque- and gingivitis-free dentitions. During the second and fourth periods (test and control periods) the participants were not allowed to brush their teeth but rinsed their mouths three times a day with Listerine or a placebo mouthwash. Plaque Index, Gingival Index, gingival fluid flow, and crevicular leukocytes were assessed on d 0, 2, 4, 7, and 14. On d 7 and 14, dental plaque was removed from the right and left jaws respectively and the wet weights determined. The chemotactic activity elaborated by the plaques was studied in Boyden chambers. During the Listerine test period, significantly lower Plaque and Gingival Index values were scored and lower amounts of plaque could be sampled in comparison to the control period."}
{"id": "PMID:1094529", "title": "An immunofluorescence study on the cross-reaction between strep. 2a and human oral mucosa.", "content": "Immunologic cross-reactions between Strep. 2A, fetal human oral mucosa (FHOM), and adult human oral mucosa (AHOM) were investigated by the use of a double layer immunofluorescence staining technique. Rabbit sera were prepared against Strep. 2A and FHOM. Seventeen sera from patients with recurrent aphthous stomatitis (RAS) and with known antibodies against Strep. 2A and AHOM were examined for antibodies against FHOM. The distribution of endpoint titers against FHOM in the sera from RAS differed significantly from the distribution in 17 controls. The immune absorptions in the rabbit sera and in the 17 sera from patients with RAS indicate that some cross-reacting antigenic determinants are shared between Strep. 2A, FHOM, and AHOM. Further, the present results indicate that antibodies are produced against antigenic determinants which are not shared by Strep. 2A, FHOM, and AHOM. The role of cross-reacting antigens in the pathogenesis of RAS is discussed but remains obscure.", "contents": "An immunofluorescence study on the cross-reaction between strep. 2a and human oral mucosa. Immunologic cross-reactions between Strep. 2A, fetal human oral mucosa (FHOM), and adult human oral mucosa (AHOM) were investigated by the use of a double layer immunofluorescence staining technique. Rabbit sera were prepared against Strep. 2A and FHOM. Seventeen sera from patients with recurrent aphthous stomatitis (RAS) and with known antibodies against Strep. 2A and AHOM were examined for antibodies against FHOM. The distribution of endpoint titers against FHOM in the sera from RAS differed significantly from the distribution in 17 controls. The immune absorptions in the rabbit sera and in the 17 sera from patients with RAS indicate that some cross-reacting antigenic determinants are shared between Strep. 2A, FHOM, and AHOM. Further, the present results indicate that antibodies are produced against antigenic determinants which are not shared by Strep. 2A, FHOM, and AHOM. The role of cross-reacting antigens in the pathogenesis of RAS is discussed but remains obscure."}
{"id": "PMID:1094530", "title": "Formation of an experimental dental cuticle.", "content": "Slices of dental enamel, cementum and dentin, and segments of epoxy resin splints with exposed synthetic hydroxyapatite crystals were incubated in human serum overnight. Observations by light and electron microscopy revealed that a cuticular structure with staining reactions and ultrastructural appearance similar to those of dental cuticle had formed on the various surfaces. The results support the hypothesis that the dental cuticle in vivo may consist of serum components which have been adsorbed to the tooth surface as a result of gingival exudation.", "contents": "Formation of an experimental dental cuticle. Slices of dental enamel, cementum and dentin, and segments of epoxy resin splints with exposed synthetic hydroxyapatite crystals were incubated in human serum overnight. Observations by light and electron microscopy revealed that a cuticular structure with staining reactions and ultrastructural appearance similar to those of dental cuticle had formed on the various surfaces. The results support the hypothesis that the dental cuticle in vivo may consist of serum components which have been adsorbed to the tooth surface as a result of gingival exudation."}
{"id": "PMID:1094532", "title": "Effect of acid in the duodenal bulb on acid and pepsin responses to test meal in dogs.", "content": "Dogs were provided with innervated pouches of the fundic stomach and of the duodenal bulb. Gastric secretion was stimulated by a test meal. Bulbar perfusion with 0.1 N HCl profoundly ibhibited the secretion of acid. Also  pepsin output and concentration of pepsin in gastric juice were reduced by bulbar acidification. The results may mean that reduction of the intrabulbar pH also plays a role in regulation of the secretion of pepsin from the stomach.", "contents": "Effect of acid in the duodenal bulb on acid and pepsin responses to test meal in dogs. Dogs were provided with innervated pouches of the fundic stomach and of the duodenal bulb. Gastric secretion was stimulated by a test meal. Bulbar perfusion with 0.1 N HCl profoundly ibhibited the secretion of acid. Also  pepsin output and concentration of pepsin in gastric juice were reduced by bulbar acidification. The results may mean that reduction of the intrabulbar pH also plays a role in regulation of the secretion of pepsin from the stomach."}
{"id": "PMID:1094533", "title": "Effect of urecholine and carbacholine on pentagastrin-stimulated gastric secretion after proximal gastric vagotomy in duodenal ulcer patients;.", "content": "Six months after proximal gastric vagotomy gastric secretion was examined after infusion of pentagastrin, 15 mug/kg/hr, alone and in combination with urecholine, 60 mug/kg/hr, or carbacholine, 2 mug/kg/hr. There were no significant differences between mean acid outputs after the three types of stimulation. Pepsin outputs were significantly higher after pentagastrin plus carbacholine and pentagastrin plus urecholine than after pentagastrin alone. Urecholine and carbacholine increased pepsin secretion to a similar degree. Volume of gastric juice was significantly higher after simultaneous infusion of pentagastrin and urecholine than after pentagastrin alone or pentagastrin plus carbacholine.", "contents": "Effect of urecholine and carbacholine on pentagastrin-stimulated gastric secretion after proximal gastric vagotomy in duodenal ulcer patients;. Six months after proximal gastric vagotomy gastric secretion was examined after infusion of pentagastrin, 15 mug/kg/hr, alone and in combination with urecholine, 60 mug/kg/hr, or carbacholine, 2 mug/kg/hr. There were no significant differences between mean acid outputs after the three types of stimulation. Pepsin outputs were significantly higher after pentagastrin plus carbacholine and pentagastrin plus urecholine than after pentagastrin alone. Urecholine and carbacholine increased pepsin secretion to a similar degree. Volume of gastric juice was significantly higher after simultaneous infusion of pentagastrin and urecholine than after pentagastrin alone or pentagastrin plus carbacholine."}
{"id": "PMID:1094534", "title": "Mechanism of B-cell activation and paralysis by thymus-independent antigens. Additive effects between NNP-LPS and LPS in the specific response to the hapten.", "content": "Normal spleen cells showed a bell-shaped dose response profile when stimulated in vitro with the thymus-independent antigen (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP)-lipopolysaccharide (LPS) with regard to the development of high-avidity plaque-forming cells to NNP. The addition of suboptimal concentrations of LPS to cultures stimulated by suboptimal concentrations of NNP-LPS resulted in optimal induction of B cells in that affinity fraction. Addition of LPS to cultures optimally stimulated by NNP-LPS resulted in paralysis of the specific cells. These results are interpreted in terms of the additive effects between the mitogenicity of LPS and the mitogenicity of NNP-LPS, the latter being selectively focused on the specific cells, thus providing further evidence for the 'one nonspecific signal' hypothesis for immune activation of B cells.", "contents": "Mechanism of B-cell activation and paralysis by thymus-independent antigens. Additive effects between NNP-LPS and LPS in the specific response to the hapten. Normal spleen cells showed a bell-shaped dose response profile when stimulated in vitro with the thymus-independent antigen (4-hydroxy-3,5-dinitrophenyl)acetyl (NNP)-lipopolysaccharide (LPS) with regard to the development of high-avidity plaque-forming cells to NNP. The addition of suboptimal concentrations of LPS to cultures stimulated by suboptimal concentrations of NNP-LPS resulted in optimal induction of B cells in that affinity fraction. Addition of LPS to cultures optimally stimulated by NNP-LPS resulted in paralysis of the specific cells. These results are interpreted in terms of the additive effects between the mitogenicity of LPS and the mitogenicity of NNP-LPS, the latter being selectively focused on the specific cells, thus providing further evidence for the 'one nonspecific signal' hypothesis for immune activation of B cells."}
{"id": "PMID:1094537", "title": "Stimulation-produced analgesia: development of tolerance and cross-tolerance to morphine.", "content": "Analgesia resulting from focal electrical stimulation of the brain of the rat shows tolerance with repeated exposures; this tolerance dissipates after a period of nonstimulation. Addiction to morphine reduces greatly the analgesia produced by electrical stimulation of the brain, which demonstrates cross-tolerance between morphine analgesia and stimulation-produced analgesia. Recovery of the stimulation-produced analgesia is seen after discontinuing administration of morphine. These results suggest that morphine and electrical stimulation produce analgesia by common mechanisms. The fact that tolerance occurs to the analgesic effect of electrical stimulation indicates that tolerance may be an alteration of an endogenous neuronal process.", "contents": "Stimulation-produced analgesia: development of tolerance and cross-tolerance to morphine. Analgesia resulting from focal electrical stimulation of the brain of the rat shows tolerance with repeated exposures; this tolerance dissipates after a period of nonstimulation. Addiction to morphine reduces greatly the analgesia produced by electrical stimulation of the brain, which demonstrates cross-tolerance between morphine analgesia and stimulation-produced analgesia. Recovery of the stimulation-produced analgesia is seen after discontinuing administration of morphine. These results suggest that morphine and electrical stimulation produce analgesia by common mechanisms. The fact that tolerance occurs to the analgesic effect of electrical stimulation indicates that tolerance may be an alteration of an endogenous neuronal process."}
{"id": "PMID:1094539", "title": "Sleep cycle oscillation: reciprocal discharge by two brainstem neuronal groups.", "content": "During the sleep cycle in cats, neurons localized to the posterolateral pole of the nucleus locus coeruleus and the nucleus subcoeruleus undergo discharge rate changes that are the opposite of those of the pontine reticular giant cells. The inverse rate ratios and activity curves of these two interconnected populations are compatible with reciprocal interaction as a physiological basis of sleep cycle oscillation.", "contents": "Sleep cycle oscillation: reciprocal discharge by two brainstem neuronal groups. During the sleep cycle in cats, neurons localized to the posterolateral pole of the nucleus locus coeruleus and the nucleus subcoeruleus undergo discharge rate changes that are the opposite of those of the pontine reticular giant cells. The inverse rate ratios and activity curves of these two interconnected populations are compatible with reciprocal interaction as a physiological basis of sleep cycle oscillation."}
{"id": "PMID:1094540", "title": "Superior colliculus: visuotopic-somatotopic overlap.", "content": "A laminar organization was present in the superior colliculus of the cat, with upper layer cells exclusively visual, lower layer cells primarily somatic (or acoustic), and intermediate layers showing significant modality overlap. The close topographic correspondence between the visual and somatic representations observed within this laminar pattern and the similarities in visual and somatic response specificity may be consistent with the hypothesis that the colliculus combines several sensory modalities to facilitate tracking of a given stimulus.", "contents": "Superior colliculus: visuotopic-somatotopic overlap. A laminar organization was present in the superior colliculus of the cat, with upper layer cells exclusively visual, lower layer cells primarily somatic (or acoustic), and intermediate layers showing significant modality overlap. The close topographic correspondence between the visual and somatic representations observed within this laminar pattern and the similarities in visual and somatic response specificity may be consistent with the hypothesis that the colliculus combines several sensory modalities to facilitate tracking of a given stimulus."}
{"id": "PMID:1094543", "title": "Hallux valgus and metatarsus primus varus treated by bunionectomy and proximal metatarsal osteotomy.", "content": "Sixteen feet with hallux valgus and metatarsus primus varus have been treated by excision of the exostosis of the first metatarsal head, advancement of the medial capsule and the abductor hallucis, release of the lateral capsule and adductor tendon, and open-wedge osteotomy at the  base of the first metatarsal inserting a bone graft. During a three-year period ther have been no nonunions of the osteotomy, and satisfactory maintenance of the corrections has occurred. This method is preferred over distal metatarsal osteotomy.", "contents": "Hallux valgus and metatarsus primus varus treated by bunionectomy and proximal metatarsal osteotomy. Sixteen feet with hallux valgus and metatarsus primus varus have been treated by excision of the exostosis of the first metatarsal head, advancement of the medial capsule and the abductor hallucis, release of the lateral capsule and adductor tendon, and open-wedge osteotomy at the  base of the first metatarsal inserting a bone graft. During a three-year period ther have been no nonunions of the osteotomy, and satisfactory maintenance of the corrections has occurred. This method is preferred over distal metatarsal osteotomy."}
{"id": "PMID:1094544", "title": "Alleviating agitation, apprehension, and related symptoms in geriatric patients: A double-blind comparison of a phenothiazine and a benzodiazepien.", "content": "A four-week, double-blind study compared the effectiveness of thioridazine and diazepam in 40 geriatric patients with various symptoms of senility. Patients' progress was evaluated with the Hamilton Anxiety Rating Scale, a Modified Nurses' Observation Scale for Inpatient Evaluation (NOSIE), a global rating of degree of illness, and a global rating of degree of overall change. The data indicate that thioridazine was superior to diazepam inrelieving troublesome symptoms of senility.", "contents": "Alleviating agitation, apprehension, and related symptoms in geriatric patients: A double-blind comparison of a phenothiazine and a benzodiazepien. A four-week, double-blind study compared the effectiveness of thioridazine and diazepam in 40 geriatric patients with various symptoms of senility. Patients' progress was evaluated with the Hamilton Anxiety Rating Scale, a Modified Nurses' Observation Scale for Inpatient Evaluation (NOSIE), a global rating of degree of illness, and a global rating of degree of overall change. The data indicate that thioridazine was superior to diazepam inrelieving troublesome symptoms of senility."}
{"id": "PMID:1094562", "title": "Type IIa hyperlipoproteinaemia. An evaluation of four therapeutic regimens.", "content": "The cholesterol-lowering effect of clofibrate (Ciba-SU13437) cholestyramine and the combination of clofibrate and cholestyramine is studied in 19 subjects with type lla hyperlipoproteinaemia, over a 20-week period. Each regimen is evaluated in terms of total cholesterol-lowering effect, cholesterol-lowering effect at 4, 8 and 16 weeks of therapy, and the incidence of side-effects. Cholestyramine is identified as the agent of choice in the management of this disorder and recommendations for its optimal utilisation are put forward.", "contents": "Type IIa hyperlipoproteinaemia. An evaluation of four therapeutic regimens. The cholesterol-lowering effect of clofibrate (Ciba-SU13437) cholestyramine and the combination of clofibrate and cholestyramine is studied in 19 subjects with type lla hyperlipoproteinaemia, over a 20-week period. Each regimen is evaluated in terms of total cholesterol-lowering effect, cholesterol-lowering effect at 4, 8 and 16 weeks of therapy, and the incidence of side-effects. Cholestyramine is identified as the agent of choice in the management of this disorder and recommendations for its optimal utilisation are put forward."}
{"id": "PMID:1094563", "title": "The role of the autopsy in modern medical practice. A review.", "content": "The role of the autopsy in modern medical practice is reviewed in the light of waning clinical interest. After a historical introduction, the reasons for this lessening interest in the autopsy, its values and its limitations are discussed, and followed by consideration of the possible future pattern of postmortem investigation.", "contents": "The role of the autopsy in modern medical practice. A review. The role of the autopsy in modern medical practice is reviewed in the light of waning clinical interest. After a historical introduction, the reasons for this lessening interest in the autopsy, its values and its limitations are discussed, and followed by consideration of the possible future pattern of postmortem investigation."}
{"id": "PMID:1094565", "title": "The use of pyrithioxine in a group of visually handicapped children. A preliminary report from the School for the Blind, Worcester.", "content": "A double-blind trial with pyrithioxine (Encephabol) was made witn 22 pairs of visually handicapped subjects who were equal in IQ scores and in chronological age. Although an improvement of abstraction ability was noticed, the over-all results were not in concurrence with the general tendency in relevant literature. A follow-up cross-over study is suggested.", "contents": "The use of pyrithioxine in a group of visually handicapped children. A preliminary report from the School for the Blind, Worcester. A double-blind trial with pyrithioxine (Encephabol) was made witn 22 pairs of visually handicapped subjects who were equal in IQ scores and in chronological age. Although an improvement of abstraction ability was noticed, the over-all results were not in concurrence with the general tendency in relevant literature. A follow-up cross-over study is suggested."}
{"id": "PMID:1094573", "title": "Shock lung: fact or fancy?", "content": "Shock lung can be considered a relatively new pathologic syndrome. Its rapidly increasing incidence is a tribute to advanced sophistication in the salvage of severely traumatized patients and in those undergoing major surgery who would not have survived 25 years ago. No single factor can be credited as the sole precipitator of this syndrome. Alone, many of the factors may be insignificant; but combined they are often synergistic and end in a respiratory death. Although all of the mechanisms and interrelationships are not known for every case, early recognition of the specific contributing etiologies aid in both the prevention and, when that fails, in the treatment of shock lung. Today, by careful precise diagnostic monitoring, many cases of post-traumatic pulmonary insufficiency, i.e., shock lung, can be treated with resultant cure. The term shock lung is valuable only in alerting the physician to the fact that the patient is in \"big trouble.\" The physician must then try to sort out all of the possible factors and treat each accordingly. We might compare the end result of shock lung to the end stage of renal disease. The fact is that they are both nonspecific responses of an end organ to many different and interrelated etiologies. To expect to define a single, all encompassing etiology is pure fancy.", "contents": "Shock lung: fact or fancy? Shock lung can be considered a relatively new pathologic syndrome. Its rapidly increasing incidence is a tribute to advanced sophistication in the salvage of severely traumatized patients and in those undergoing major surgery who would not have survived 25 years ago. No single factor can be credited as the sole precipitator of this syndrome. Alone, many of the factors may be insignificant; but combined they are often synergistic and end in a respiratory death. Although all of the mechanisms and interrelationships are not known for every case, early recognition of the specific contributing etiologies aid in both the prevention and, when that fails, in the treatment of shock lung. Today, by careful precise diagnostic monitoring, many cases of post-traumatic pulmonary insufficiency, i.e., shock lung, can be treated with resultant cure. The term shock lung is valuable only in alerting the physician to the fact that the patient is in \"big trouble.\" The physician must then try to sort out all of the possible factors and treat each accordingly. We might compare the end result of shock lung to the end stage of renal disease. The fact is that they are both nonspecific responses of an end organ to many different and interrelated etiologies. To expect to define a single, all encompassing etiology is pure fancy."}
{"id": "PMID:1094575", "title": "Donor-specific IgG antibody and the chronic rejection of human renal allografts.", "content": "Although many investigators have felt that humoral antibody was responsible for chronic rejection, attempts to detect it in the sera of recipients in the presence of functioning renal allografts have been largely unsuccessful. A modification of the mixed antiglobulin reaction has increased its sensitivity so that the development of low titers of immunoglobulin (IgG) antibody antibody specific for donor kidney cells can be detected in renal allograft recipients while renal function is still good. Donor-specific antibody was detected in the sera of 11 of 13 patients whose transplants had ceased to function from 5 to 43 months after transplantation. In five recipients the antibody was present prior to as well as after transplantation and in six recipients antibody developed after transplantation from 3 to 25 months prior to the cessation of function. In the patients with antibody, chronic rejection was characterized by hypertension which required treatment with multiple drugs, by proteinuria of greater than one gram per day, by a gradual, progressively rising serum creatinine, and by an absence of acute ologuric rejection episodes. Pathologically there was extensive intimal proliferation and occlusion of the intrarenal arteris. There also was significant glomerulonephritis which consisted of thickening of the basement membranes, mesangial cell proliferation, simplification of the capillary loops, and in some patients fibroepithelial crescent formation. These findings suggest that IgG antibodies directed against cell-surface antigens of the donor are the chief cause of chronic renal allograft rejection.", "contents": "Donor-specific IgG antibody and the chronic rejection of human renal allografts. Although many investigators have felt that humoral antibody was responsible for chronic rejection, attempts to detect it in the sera of recipients in the presence of functioning renal allografts have been largely unsuccessful. A modification of the mixed antiglobulin reaction has increased its sensitivity so that the development of low titers of immunoglobulin (IgG) antibody antibody specific for donor kidney cells can be detected in renal allograft recipients while renal function is still good. Donor-specific antibody was detected in the sera of 11 of 13 patients whose transplants had ceased to function from 5 to 43 months after transplantation. In five recipients the antibody was present prior to as well as after transplantation and in six recipients antibody developed after transplantation from 3 to 25 months prior to the cessation of function. In the patients with antibody, chronic rejection was characterized by hypertension which required treatment with multiple drugs, by proteinuria of greater than one gram per day, by a gradual, progressively rising serum creatinine, and by an absence of acute ologuric rejection episodes. Pathologically there was extensive intimal proliferation and occlusion of the intrarenal arteris. There also was significant glomerulonephritis which consisted of thickening of the basement membranes, mesangial cell proliferation, simplification of the capillary loops, and in some patients fibroepithelial crescent formation. These findings suggest that IgG antibodies directed against cell-surface antigens of the donor are the chief cause of chronic renal allograft rejection."}
{"id": "PMID:1094576", "title": "Liver failure in the postoperative patient: the role of sepsis and immunologic deficiency.", "content": "In a 4 year experience with 7- postoperative or postinjury patients in liver failure, we found that 27 (37 percent) had associated bacterial sepsis. The mortality rate in this group of patients was 48 percent, compared with an average of 80 percent among patients with liver failure from other causes. Laboratory evaluation of cellular immune response in 20 cirrhotic patients confirmed the clinical observation of a relative failure of cellular immunity. Mitogen response of lymphocytes to phytohemagglutinin was significantly decreased in cirrhotic patients as was the number of thymus-derived lymphocytes. The association and poor prognosis of pre-existing liver damage, injury, bacterial sepsis, and liver failure is discussed. It is concluded that a postoperative or postinjury patient, regardless of his general condition, who has any possibility of having undrained intraperitoneal sepsis, deserves re-exploration since the trade-off for benefit usually far outweighs the possibility of doing harm. The decision for reoperation in such critically ill patients is not easy, but such operative aggressiveness is warranted.", "contents": "Liver failure in the postoperative patient: the role of sepsis and immunologic deficiency. In a 4 year experience with 7- postoperative or postinjury patients in liver failure, we found that 27 (37 percent) had associated bacterial sepsis. The mortality rate in this group of patients was 48 percent, compared with an average of 80 percent among patients with liver failure from other causes. Laboratory evaluation of cellular immune response in 20 cirrhotic patients confirmed the clinical observation of a relative failure of cellular immunity. Mitogen response of lymphocytes to phytohemagglutinin was significantly decreased in cirrhotic patients as was the number of thymus-derived lymphocytes. The association and poor prognosis of pre-existing liver damage, injury, bacterial sepsis, and liver failure is discussed. It is concluded that a postoperative or postinjury patient, regardless of his general condition, who has any possibility of having undrained intraperitoneal sepsis, deserves re-exploration since the trade-off for benefit usually far outweighs the possibility of doing harm. The decision for reoperation in such critically ill patients is not easy, but such operative aggressiveness is warranted."}
{"id": "PMID:1094577", "title": "Hepatic portoenterostomy (the Kasai operation) for biliary atresia.", "content": "Twenty-two infants and children with extrahepatic biliary atresia were treated by Kasai's hepatic portoenterostomy operation since 1972. Postoperative bile drainage occurred in 11 infants. Success never was obtained in patients older than 12 weeks nor in the absence of microscopic biliary ductules in the excised fibrotic remnant of the extrahepatic bile ducts. With these exclusions almost 90 percent of infants with biliary atresia had sustained bile drainage after operation. In the early cases, ascending cholangitis was a major and recurrent complication with sequellae in many instances of permanent jaundice and probably irreversible liver damage. A new and simple modification of Kasai's original operation has prevented this complication thus far. Despite apparently normal bile drainage, postoperative studies by transhepatic cholangiography in some of these patients have demonstrated a grossly abnormal hepatobiliary intrastructure, suggesting that an underlying liver involvement may be present in biliary atresia.", "contents": "Hepatic portoenterostomy (the Kasai operation) for biliary atresia. Twenty-two infants and children with extrahepatic biliary atresia were treated by Kasai's hepatic portoenterostomy operation since 1972. Postoperative bile drainage occurred in 11 infants. Success never was obtained in patients older than 12 weeks nor in the absence of microscopic biliary ductules in the excised fibrotic remnant of the extrahepatic bile ducts. With these exclusions almost 90 percent of infants with biliary atresia had sustained bile drainage after operation. In the early cases, ascending cholangitis was a major and recurrent complication with sequellae in many instances of permanent jaundice and probably irreversible liver damage. A new and simple modification of Kasai's original operation has prevented this complication thus far. Despite apparently normal bile drainage, postoperative studies by transhepatic cholangiography in some of these patients have demonstrated a grossly abnormal hepatobiliary intrastructure, suggesting that an underlying liver involvement may be present in biliary atresia."}
{"id": "PMID:1094584", "title": "[Late results of replacement of the antrio-ventricular valve].", "content": "From 1966 to 1969 mitral-valve-allografts have been implanted for mitral valve replacement in 11 and for tricuspid valve replacement in two patients. Three patients died immediately after the operation. Eigth allografts in mitral position and one in tricuspid position functioned well. One case was complicated by candida albicans. There were no thromboembolic complications. Long-term follow up revealed late deterioration of allograft function in every case. Four allografts have been replaced four are still functioning four to six years postoperatively. Because of the poor durability of the results this method of mitral valve replacement is not used any more.", "contents": "[Late results of replacement of the antrio-ventricular valve]. From 1966 to 1969 mitral-valve-allografts have been implanted for mitral valve replacement in 11 and for tricuspid valve replacement in two patients. Three patients died immediately after the operation. Eigth allografts in mitral position and one in tricuspid position functioned well. One case was complicated by candida albicans. There were no thromboembolic complications. Long-term follow up revealed late deterioration of allograft function in every case. Four allografts have been replaced four are still functioning four to six years postoperatively. Because of the poor durability of the results this method of mitral valve replacement is not used any more."}
{"id": "PMID:1094585", "title": "[Rastelli operation for complete transposition of the great vessels, ventricle septum defect and pulmonary atresis].", "content": "We report about a 6 year old patient with complete transpositon of the great arteries, a ventiricle septum defect, and pulmonary atresia, who was operated using Rastellis technique. A Hancock-prothesis of of 20 mm diameter was used for  reconstruction of the outflow tract of the right  ventricle and anastomosed distal with the pulmonary artery on the left side of the aorta. The proximal anastomosis was performed with the right ventricle after a longitudinal ventriculotomy. The intraoperative pressure recordings and the post-operative studies revealed a very good early result.", "contents": "[Rastelli operation for complete transposition of the great vessels, ventricle septum defect and pulmonary atresis]. We report about a 6 year old patient with complete transpositon of the great arteries, a ventiricle septum defect, and pulmonary atresia, who was operated using Rastellis technique. A Hancock-prothesis of of 20 mm diameter was used for  reconstruction of the outflow tract of the right  ventricle and anastomosed distal with the pulmonary artery on the left side of the aorta. The proximal anastomosis was performed with the right ventricle after a longitudinal ventriculotomy. The intraoperative pressure recordings and the post-operative studies revealed a very good early result."}
{"id": "PMID:1094591", "title": "Short term effects of ionizing radiation on the recurrent or metastatic skin cancer--a quantitative DNA study.", "content": "A total of 72 smears from two cases of metastatic squamous cell carcinoma of the head and neck during radiation therapy were studied for DNA content.  The percentage of aneuploid tumor cells in the range of lower ploidy was high in both cases and remained high throughout the treatment.  The correlation between the percentage of aneuploid cells in metastatic squamous cell carcinoma and radiocurability of these lesions has been discussed.", "contents": "Short term effects of ionizing radiation on the recurrent or metastatic skin cancer--a quantitative DNA study. A total of 72 smears from two cases of metastatic squamous cell carcinoma of the head and neck during radiation therapy were studied for DNA content.  The percentage of aneuploid tumor cells in the range of lower ploidy was high in both cases and remained high throughout the treatment.  The correlation between the percentage of aneuploid cells in metastatic squamous cell carcinoma and radiocurability of these lesions has been discussed."}
{"id": "PMID:1094593", "title": "Total body irradiation in non-Hodgkin lymphoma.", "content": "17 patients with non-Hodgkin lymphomas were treated by total body irradiation (TBI). 94% went into remission. Response in lymphosarcomas was found to be extremely good with long periods of unmaintained remission. Reticulumcell sarcomas responded poorly.", "contents": "Total body irradiation in non-Hodgkin lymphoma. 17 patients with non-Hodgkin lymphomas were treated by total body irradiation (TBI). 94% went into remission. Response in lymphosarcomas was found to be extremely good with long periods of unmaintained remission. Reticulumcell sarcomas responded poorly."}
{"id": "PMID:1094597", "title": "[Forensic dentistry and its position in the service of the justice].", "content": "Forensic stomatology makes the findings of dental research available to justice. Thus, it is a branch of the forensic sciences. Among the main tasks of forensic stomatology are the identification of people and (in crimes of violence, especially sex crimes) the statement, analysis and evaluation of bite marks. The marking of dentures is also dealt with.", "contents": "[Forensic dentistry and its position in the service of the justice]. Forensic stomatology makes the findings of dental research available to justice. Thus, it is a branch of the forensic sciences. Among the main tasks of forensic stomatology are the identification of people and (in crimes of violence, especially sex crimes) the statement, analysis and evaluation of bite marks. The marking of dentures is also dealt with."}
{"id": "PMID:1094635", "title": "A heterophile system in human renal transplantation. IV. Natural immunity and its genetic implications.", "content": "Natural heterophile immunity was studied in 949 individuals including 41 recipients and donors of renal transplants and 141 families. The prevalence and strength of the natural immunity was at its maximum during the first two decades of life and declined thereafter. The population could be divided into three groups: one with natural immunity to heterophile transplantation antigens (HT-A), one with a heterophile immunity which was not (anti-HT-A) (i.e., anti-HX-A), and one group which had no heterophile immunity. Transplantation among these groups yielded results which along with family studies and mathematical considerations suggest that the HT-A system is controlled by a single genetic locus comprised of one dominant and one recessive allele.", "contents": "A heterophile system in human renal transplantation. IV. Natural immunity and its genetic implications. Natural heterophile immunity was studied in 949 individuals including 41 recipients and donors of renal transplants and 141 families. The prevalence and strength of the natural immunity was at its maximum during the first two decades of life and declined thereafter. The population could be divided into three groups: one with natural immunity to heterophile transplantation antigens (HT-A), one with a heterophile immunity which was not (anti-HT-A) (i.e., anti-HX-A), and one group which had no heterophile immunity. Transplantation among these groups yielded results which along with family studies and mathematical considerations suggest that the HT-A system is controlled by a single genetic locus comprised of one dominant and one recessive allele."}
{"id": "PMID:1094636", "title": "Soluble membrane antigen fractions that react with rat enhancing alloantibodies.", "content": "Soluble extracts were prepared from cells of Brown Norway (BN) rat spleens by sonication in 2 M KI. Extracts were fractionated by gel filtration and electrophoretic procedures. The fractions specifically inhibited the binding of Lewis alloantibodies to target BN cells in vitro and in vivo. A homogeneous component that retained antigen activity was obtained by preparatory polyacrylamide gel electrophoresis. Lewis rats injected with BN antigen fractions produced histocompatibility alloantisera that would prolong the survival of BN skin grafts applied to Lewis neonates.", "contents": "Soluble membrane antigen fractions that react with rat enhancing alloantibodies. Soluble extracts were prepared from cells of Brown Norway (BN) rat spleens by sonication in 2 M KI. Extracts were fractionated by gel filtration and electrophoretic procedures. The fractions specifically inhibited the binding of Lewis alloantibodies to target BN cells in vitro and in vivo. A homogeneous component that retained antigen activity was obtained by preparatory polyacrylamide gel electrophoresis. Lewis rats injected with BN antigen fractions produced histocompatibility alloantisera that would prolong the survival of BN skin grafts applied to Lewis neonates."}
{"id": "PMID:1094637", "title": "Comparison of kidney transplant survival among transplant centers.", "content": "Human kidney transplant survival rates at 1 year were compared among centers according to the transplantation activity as measured by the number of renal transplants performed. Statistical methods are described by which a fair assessment can be made of the outcome when small numbers of grafts are involved. The method should also be useful in evaluating subsets of patients in any given center under different treatment protocols. No evidence was found that survival rates of cadaver donor transplants were lower at the smaller centers. Other factors besides size of centers are probably of greater importance in influencing transplant survival rates.", "contents": "Comparison of kidney transplant survival among transplant centers. Human kidney transplant survival rates at 1 year were compared among centers according to the transplantation activity as measured by the number of renal transplants performed. Statistical methods are described by which a fair assessment can be made of the outcome when small numbers of grafts are involved. The method should also be useful in evaluating subsets of patients in any given center under different treatment protocols. No evidence was found that survival rates of cadaver donor transplants were lower at the smaller centers. Other factors besides size of centers are probably of greater importance in influencing transplant survival rates."}
{"id": "PMID:1094638", "title": "Effect of time on sensitization to hemophoietic grafts by preceding blood transfusion.", "content": "Previous studies have shown that a single transfusion with whole blood from the intended marrow donor 10 days before 1,200 R of total body irradiation (TBI) and marrow grafting can immunize a dog and lead to rejection of the subsequent marrow graft. The present study explored the effect of time on immunization to marrow grafts by preceding blood transfusion. All receipents were given 1,200 R of TBI followed with 4 hr by a hemopoietic graft from an unrelated donor mismatched at the major canine histocompatibility complex. Two groups of recipients were studed. In group 1, 7 dogs were given a transfusion of blood from the marrow donor 24 hr before TBI, and 6 rejected the graft; in group 2, 16 dogs were given transfusion of blood from the marrow donor 3 months before TBI, and 8 rejected the graft. The frequency of rejection in both groups was significantly greater than in untransfused dogs mismatched with their donors at the canine major histocompatibility complex (11 rejections in 67 transplants). The results indicate that exposure to donor blood from 24 hr to 3 months before marrow grafting significantly increases the likelihood of graft rejection.", "contents": "Effect of time on sensitization to hemophoietic grafts by preceding blood transfusion. Previous studies have shown that a single transfusion with whole blood from the intended marrow donor 10 days before 1,200 R of total body irradiation (TBI) and marrow grafting can immunize a dog and lead to rejection of the subsequent marrow graft. The present study explored the effect of time on immunization to marrow grafts by preceding blood transfusion. All receipents were given 1,200 R of TBI followed with 4 hr by a hemopoietic graft from an unrelated donor mismatched at the major canine histocompatibility complex. Two groups of recipients were studed. In group 1, 7 dogs were given a transfusion of blood from the marrow donor 24 hr before TBI, and 6 rejected the graft; in group 2, 16 dogs were given transfusion of blood from the marrow donor 3 months before TBI, and 8 rejected the graft. The frequency of rejection in both groups was significantly greater than in untransfused dogs mismatched with their donors at the canine major histocompatibility complex (11 rejections in 67 transplants). The results indicate that exposure to donor blood from 24 hr to 3 months before marrow grafting significantly increases the likelihood of graft rejection."}
{"id": "PMID:1094645", "title": "Re-establishment of cutaneous leishmaniasis after cessation of anti-malaria spraying.", "content": "Decrease or interruption of transmission of cutaneous leishmaniasis as a result of spraying of human dwellings with residual insecticides has been reported by several investigators. In this report the behaviour of the disease after cessation of spraying in the villages of Isfahan is studied. In 16 villages sprayed with DDT from 1966 through 1969, the incidence decreased from 8.2 per 1000 in 1965 to 0,7 per 1000 in 1969 but in 1970, the first year after cessation of spraying, the incidence increased to 15 per 1000, a 20-fold increase. The control villages did not show these changes, still having the incidence figure of more than 9 per 1000 per year. The study shows that insecticide spraying is not the fianl answer in the control of leishmaniasis.", "contents": "Re-establishment of cutaneous leishmaniasis after cessation of anti-malaria spraying. Decrease or interruption of transmission of cutaneous leishmaniasis as a result of spraying of human dwellings with residual insecticides has been reported by several investigators. In this report the behaviour of the disease after cessation of spraying in the villages of Isfahan is studied. In 16 villages sprayed with DDT from 1966 through 1969, the incidence decreased from 8.2 per 1000 in 1965 to 0,7 per 1000 in 1969 but in 1970, the first year after cessation of spraying, the incidence increased to 15 per 1000, a 20-fold increase. The control villages did not show these changes, still having the incidence figure of more than 9 per 1000 per year. The study shows that insecticide spraying is not the fianl answer in the control of leishmaniasis."}
{"id": "PMID:1094646", "title": "[Characteristics of the morphological changes in the nuclear structures in oogenesis in holothurians].", "content": "Chromosomes and nucleoli were studied at the meiosis diplotene stage in oocytes of Stichopus japonicus and Cucumaria fraudatrix. The S. japonicus nuclear structures were found to change in the same way as in other holothurioidea (chromosomes turn into lampbrushes, there is a single rapidly increasing nucleolus). The C. fraudatrix oocytes are notable for their lampbrushes located in immediate vicinity of the nucleus membrane. The same modes of behavior of nuclear structures are known for three more types of Cucumaria (no more species have been studied). The specific features of behavior of the nuclear structures are discussed with regard to phylogeny of holothurioidea oogenesis.", "contents": "[Characteristics of the morphological changes in the nuclear structures in oogenesis in holothurians]. Chromosomes and nucleoli were studied at the meiosis diplotene stage in oocytes of Stichopus japonicus and Cucumaria fraudatrix. The S. japonicus nuclear structures were found to change in the same way as in other holothurioidea (chromosomes turn into lampbrushes, there is a single rapidly increasing nucleolus). The C. fraudatrix oocytes are notable for their lampbrushes located in immediate vicinity of the nucleus membrane. The same modes of behavior of nuclear structures are known for three more types of Cucumaria (no more species have been studied). The specific features of behavior of the nuclear structures are discussed with regard to phylogeny of holothurioidea oogenesis."}
{"id": "PMID:1094647", "title": "[Change in cellular surface structures and volume under the action of colchicine].", "content": "Effect of colchicine upon the surface structures and volume of embryonic pig kidney cells and mouse ascite carcinoma Krebs II cells involved an increase of the cells volume. This was confirmed by the scanning electron microscopy data. The surface of colchicine-treated pig cells has less microvilli due, probably, to their enlarged volume. Swelling of mitochondrial matrix as well as some other ultrastructural changes in cytoplasm of the drug-treated cells suggest that the increase in the volume is a result of cell hydration. Disturbance of the osmotic equilibrium following the depolymerization of microtubles in the mitotic spindle and cytoplasm, is discussed.", "contents": "[Change in cellular surface structures and volume under the action of colchicine]. Effect of colchicine upon the surface structures and volume of embryonic pig kidney cells and mouse ascite carcinoma Krebs II cells involved an increase of the cells volume. This was confirmed by the scanning electron microscopy data. The surface of colchicine-treated pig cells has less microvilli due, probably, to their enlarged volume. Swelling of mitochondrial matrix as well as some other ultrastructural changes in cytoplasm of the drug-treated cells suggest that the increase in the volume is a result of cell hydration. Disturbance of the osmotic equilibrium following the depolymerization of microtubles in the mitotic spindle and cytoplasm, is discussed."}
{"id": "PMID:1094649", "title": "[Basic factors in the cerebellar Purkinje cell polyploidization in chicken embyogeny. II. The changes in the content and concentration of proteins, protein SH-groups and RNA in the cytoplasm of Purkinje cells in the process of their differentiation and specialization].", "content": "Cytophotometric study of the cerebellum Purkinje cells in the chick embryos(10th-21st day) showed that the total amount of protein increased at this period proportionately to the growth of cells, its concentration remaining relatively constant. Thebasic and acid proteins ratio changed, the acid proteins increasing. Content of the protein SH-group increases considerably during the tigroid and eneurofibrillas formation, - simultaneously with the conent of RNA. The periods of intensification of the protein synthesis coincide with the morpho-functional maturation of neurons and formation of signs characterizing the maturity of the embryo as a whole. Supposedly the changesin the activity of nuclear apparatus of the cells, manifested in the increase of nuclear size and the number and size of nucleoli, underlie this process.", "contents": "[Basic factors in the cerebellar Purkinje cell polyploidization in chicken embyogeny. II. The changes in the content and concentration of proteins, protein SH-groups and RNA in the cytoplasm of Purkinje cells in the process of their differentiation and specialization]. Cytophotometric study of the cerebellum Purkinje cells in the chick embryos(10th-21st day) showed that the total amount of protein increased at this period proportionately to the growth of cells, its concentration remaining relatively constant. Thebasic and acid proteins ratio changed, the acid proteins increasing. Content of the protein SH-group increases considerably during the tigroid and eneurofibrillas formation, - simultaneously with the conent of RNA. The periods of intensification of the protein synthesis coincide with the morpho-functional maturation of neurons and formation of signs characterizing the maturity of the embryo as a whole. Supposedly the changesin the activity of nuclear apparatus of the cells, manifested in the increase of nuclear size and the number and size of nucleoli, underlie this process."}
{"id": "PMID:1094650", "title": "[Electrical characteristics of normal and transformed fibroblasts in tissue culture].", "content": "The two-electrode method of input resistance measuring was shown to give lower values of resistance compared with the one-electrode balance method. Gradual growth of estimates of input resistance and membrane potential in the cause of experiment is associated with decrease in the shunting effect of the electrode puncture. The mean values of steady Rin of sparse and confluent culture did not differ when the cells were electrically disconnected (38 plus or minus 1.2 MO and 41 plus or minus 1.8 MO respectively) and differed in electrically coupled fibroblasts (54 plus or minus 4.9 MO --sparse; 29 plus or minus 2.2 MO -- confluent culture). This difference is thought to be associated with shunting effect of electrical connections. The specific resistance of membrane was estimated in normal and transformed fibroblasts.", "contents": "[Electrical characteristics of normal and transformed fibroblasts in tissue culture]. The two-electrode method of input resistance measuring was shown to give lower values of resistance compared with the one-electrode balance method. Gradual growth of estimates of input resistance and membrane potential in the cause of experiment is associated with decrease in the shunting effect of the electrode puncture. The mean values of steady Rin of sparse and confluent culture did not differ when the cells were electrically disconnected (38 plus or minus 1.2 MO and 41 plus or minus 1.8 MO respectively) and differed in electrically coupled fibroblasts (54 plus or minus 4.9 MO --sparse; 29 plus or minus 2.2 MO -- confluent culture). This difference is thought to be associated with shunting effect of electrical connections. The specific resistance of membrane was estimated in normal and transformed fibroblasts."}
{"id": "PMID:1094651", "title": "[Quantitative characteristics of the migration ability of different types of fibroblast-like cells cultured on substrates with an ordered relief].", "content": "Reactions to the geometrical configuration of the underlying surface of fibroblast-like cells of various species (mouse, rat, chick, hamster, human) were compared quantitatively. All normal cells migrated from 25-40 mcm deep grooves but not from the less deep ones -5-15 mcm. Migration ability was the highest in the mouse cells;it was less pronounced in rat and chick cells; hamster and human cells had the lowest migration ability. The offered quantitative method can be used for estimation of the reaction to the ordered relief of the substratum of different minds of cultured cells - normal as well as pathologically changed.", "contents": "[Quantitative characteristics of the migration ability of different types of fibroblast-like cells cultured on substrates with an ordered relief]. Reactions to the geometrical configuration of the underlying surface of fibroblast-like cells of various species (mouse, rat, chick, hamster, human) were compared quantitatively. All normal cells migrated from 25-40 mcm deep grooves but not from the less deep ones -5-15 mcm. Migration ability was the highest in the mouse cells;it was less pronounced in rat and chick cells; hamster and human cells had the lowest migration ability. The offered quantitative method can be used for estimation of the reaction to the ordered relief of the substratum of different minds of cultured cells - normal as well as pathologically changed."}
{"id": "PMID:1094652", "title": "[Degradation of yeast mitochondria induced by anaerobiosis at the early stages of growth of the culture].", "content": "Prolonged anaerobiosis of Saccharomyces cerevisiae cells result in degenerative changes in mitochondria which can be revealed when measuring the enzyme activities of the respiratory chain in isolated organellas and by electron microscope examination of the cells. The sensitivity of yeast mitochondria to anaerosiosis alters as the culture growth. Mitochondria are especially labile during the early expotential growth phase. Possible reasons for and mechanism of degradation of mitochondria in vivo are discussed.", "contents": "[Degradation of yeast mitochondria induced by anaerobiosis at the early stages of growth of the culture]. Prolonged anaerobiosis of Saccharomyces cerevisiae cells result in degenerative changes in mitochondria which can be revealed when measuring the enzyme activities of the respiratory chain in isolated organellas and by electron microscope examination of the cells. The sensitivity of yeast mitochondria to anaerosiosis alters as the culture growth. Mitochondria are especially labile during the early expotential growth phase. Possible reasons for and mechanism of degradation of mitochondria in vivo are discussed."}
{"id": "PMID:1094667", "title": "Analgesic abuse syndrome: a frequently overlooked cause of reversible renal failure.", "content": "The incidence of analgesic nephropathy in the United States is greater than previously reported. Because of the characteristic radiographic features of papillary necrosis, this diagnosis may be made while it is still clinically unsuspected. Early diagnosis is extremely important because cessation of analgesic abuse may avert progressive renal damage. Uncovering the diagnosis calls for special care in obtaining the telltale history. This must be sought in patients with radiographic evidence of papillary necrosis when a history of diabetes mellitus, obstructive uropathy, or sicle cell anemia is absent, or in patients with unexplained nephrocalcinosis or nephrolithiasis.", "contents": "Analgesic abuse syndrome: a frequently overlooked cause of reversible renal failure. The incidence of analgesic nephropathy in the United States is greater than previously reported. Because of the characteristic radiographic features of papillary necrosis, this diagnosis may be made while it is still clinically unsuspected. Early diagnosis is extremely important because cessation of analgesic abuse may avert progressive renal damage. Uncovering the diagnosis calls for special care in obtaining the telltale history. This must be sought in patients with radiographic evidence of papillary necrosis when a history of diabetes mellitus, obstructive uropathy, or sicle cell anemia is absent, or in patients with unexplained nephrocalcinosis or nephrolithiasis."}
{"id": "PMID:1094668", "title": "Renal transplantation and placement of ileal stoma.", "content": "The literature has been reviewed to present the techniques used by others when renal transplantation is done in conjunction with ileal segment urinary diversion. A successful technique for the convenient placement of the ileal stoma away from the transplanted kidney in the left lower abdominal quadrant while placing the donor kidney in the right iliac fossa is described and recommended, using a fourteen-year-old patient as an illustrative case.", "contents": "Renal transplantation and placement of ileal stoma. The literature has been reviewed to present the techniques used by others when renal transplantation is done in conjunction with ileal segment urinary diversion. A successful technique for the convenient placement of the ileal stoma away from the transplanted kidney in the left lower abdominal quadrant while placing the donor kidney in the right iliac fossa is described and recommended, using a fourteen-year-old patient as an illustrative case."}
{"id": "PMID:1094669", "title": "Preliminary reticulum cell sarcoma of prostate gland.", "content": "A case of primary reticulum cell sarcoma of the prostate gland is presented. This is the seventh well-documented case we have found in the literature. No evidence of extraprostatic extension has been found two and a half years after diagnosis was made, despite no chemotherapy nor radiotherapy.", "contents": "Preliminary reticulum cell sarcoma of prostate gland. A case of primary reticulum cell sarcoma of the prostate gland is presented. This is the seventh well-documented case we have found in the literature. No evidence of extraprostatic extension has been found two and a half years after diagnosis was made, despite no chemotherapy nor radiotherapy."}
{"id": "PMID:1094672", "title": "Evaluation of an iodophor teat spray in the control of infections and cellular reactions in the udder.", "content": "A strictly controlled field trial was designed to assess the value of an iodophorpost-milking teat disinfectant in the prevention of new mastitis infections in a 132 cow herd. The disinfectant was applied by a hand spray, a method acceptable, effective, and less costly than conventional teat dipping methods. Only the left side of the udder was treated, the right quarters acting as controls. Dry-cow therapy and other methods of mastitis control were already practised, and no change in routine was introduced apart from half-udder teat spraying. Fifty-eight new cases of mastitis were found to occur on the teat-sprayed side, compared with 91 in the untreated quarters; 35 and 23, respectively, were clinical. No Group B streptococci existed in the herd, and the principal reductions were in Group C streptococci and coagulase-negative staphylococci, the percentages of which were halved. The percentage of coliform infections on the teat-sprayed side was, in contrast, doubled. The incidence of new clinical and subclinical disease without infection was in reverse ratio to that of infections, being 29-9 per cent higher in the quarters which were teat-sprayed. Analysis of cumulative CMT reactions from regular monthly surveys indicated that exponential cell-count values on each side of the udder were identical. Thus, while new infections were significantly reduced, overall cell counts were not, and there is clearly a need for further research into factors other than bacteria which may initiate teat damage and inflammatory changes in the udder.", "contents": "Evaluation of an iodophor teat spray in the control of infections and cellular reactions in the udder. A strictly controlled field trial was designed to assess the value of an iodophorpost-milking teat disinfectant in the prevention of new mastitis infections in a 132 cow herd. The disinfectant was applied by a hand spray, a method acceptable, effective, and less costly than conventional teat dipping methods. Only the left side of the udder was treated, the right quarters acting as controls. Dry-cow therapy and other methods of mastitis control were already practised, and no change in routine was introduced apart from half-udder teat spraying. Fifty-eight new cases of mastitis were found to occur on the teat-sprayed side, compared with 91 in the untreated quarters; 35 and 23, respectively, were clinical. No Group B streptococci existed in the herd, and the principal reductions were in Group C streptococci and coagulase-negative staphylococci, the percentages of which were halved. The percentage of coliform infections on the teat-sprayed side was, in contrast, doubled. The incidence of new clinical and subclinical disease without infection was in reverse ratio to that of infections, being 29-9 per cent higher in the quarters which were teat-sprayed. Analysis of cumulative CMT reactions from regular monthly surveys indicated that exponential cell-count values on each side of the udder were identical. Thus, while new infections were significantly reduced, overall cell counts were not, and there is clearly a need for further research into factors other than bacteria which may initiate teat damage and inflammatory changes in the udder."}
{"id": "PMID:1094743", "title": "[The place of ultrasound diagnosis in internal medicine].", "content": "The applications of ultrasound diagnosis within the framework of general medicine are discussed on the basis of case reports. Ultrasonic equipment with manual moving of the probe is used in this department for the investigation of the abdomen in cases of occult neoplasms and metastases to differentiate between cysts and solid tumours and to determine the situation and size of parenchymatous organs like liver and kidney and spleen. Special fields of application are: follow up of patients after renal transplantation, investigation of systemic haematological diseases such as lymphogranuloma, or the demonstration of abnormal fluid masses such as haematomata in haemophilic patients, ascites or pericardial effusions.", "contents": "[The place of ultrasound diagnosis in internal medicine]. The applications of ultrasound diagnosis within the framework of general medicine are discussed on the basis of case reports. Ultrasonic equipment with manual moving of the probe is used in this department for the investigation of the abdomen in cases of occult neoplasms and metastases to differentiate between cysts and solid tumours and to determine the situation and size of parenchymatous organs like liver and kidney and spleen. Special fields of application are: follow up of patients after renal transplantation, investigation of systemic haematological diseases such as lymphogranuloma, or the demonstration of abnormal fluid masses such as haematomata in haemophilic patients, ascites or pericardial effusions."}
{"id": "PMID:1094744", "title": "[Functional risks in the surgical therapy of urinary incontinence and prolapse (author's transl)].", "content": "In addition to the known surgical risks in the treatment of incontinence or prolapse there exists a functional risk inherent in the surgical methods, which can even cause a deterioration in the functional condition if the surgical aim is not achieved. The vaginal bladderneck sutures are only worth as much as the tissues they hold together. The surgical treatment of certain cases of prolapse can eliminate the stopcock mechanism of the urethra and lead to the manifestation of a preoperatively-existent incontinence. With the sling operations success depends, among other factors, on the degree of restraint and the correct placement of the sling. The outcome of hysterectomy is endangered by failure to recognize a coexistent prolapse or enterocoele. With Ward's manipulation, high peritonization and adequate treatment of the overextened posterior vaginal vault, the involved functional risks can be minimized. Typical examples are mentioned in the text. Particular emphasis is placed on the necessity of exact knowledge of the circumstances threatending the functional success and their recognition by means of wick-urethrocystocolporectography. For the maintenance of a good doctor-patient relationship, the patient may receive prior to surgery an understandable explanation of the limitations of the methods used in achieving a functional cure.", "contents": "[Functional risks in the surgical therapy of urinary incontinence and prolapse (author's transl)]. In addition to the known surgical risks in the treatment of incontinence or prolapse there exists a functional risk inherent in the surgical methods, which can even cause a deterioration in the functional condition if the surgical aim is not achieved. The vaginal bladderneck sutures are only worth as much as the tissues they hold together. The surgical treatment of certain cases of prolapse can eliminate the stopcock mechanism of the urethra and lead to the manifestation of a preoperatively-existent incontinence. With the sling operations success depends, among other factors, on the degree of restraint and the correct placement of the sling. The outcome of hysterectomy is endangered by failure to recognize a coexistent prolapse or enterocoele. With Ward's manipulation, high peritonization and adequate treatment of the overextened posterior vaginal vault, the involved functional risks can be minimized. Typical examples are mentioned in the text. Particular emphasis is placed on the necessity of exact knowledge of the circumstances threatending the functional success and their recognition by means of wick-urethrocystocolporectography. For the maintenance of a good doctor-patient relationship, the patient may receive prior to surgery an understandable explanation of the limitations of the methods used in achieving a functional cure."}
{"id": "PMID:1094745", "title": "[Equilin sulphate in the treatment of the menopausal syndrome (author's transl)].", "content": "0.25 sodium equilin sulphate and 0.625 mg Premarin were compared as to their effects in 12 postmenopausal syndrome. The initial daily dosage of 0.25 mg equilin had a stronger oestrongenic effect in the alleviation of vasomotor disturbances than subsequently-administered Premarin. No significant difference was found in effect on the vaginal epithelium or the endometrium or in the incidence of irregular bleeding. The number of women in whom withdrawal bleeding was reported was considerably reduced by lowering the dosage to 0.2 mg equilin sulphate. Consequently 0.2 to 0.3 mg equilin sulphate must be considered the optimum dosage.", "contents": "[Equilin sulphate in the treatment of the menopausal syndrome (author's transl)]. 0.25 sodium equilin sulphate and 0.625 mg Premarin were compared as to their effects in 12 postmenopausal syndrome. The initial daily dosage of 0.25 mg equilin had a stronger oestrongenic effect in the alleviation of vasomotor disturbances than subsequently-administered Premarin. No significant difference was found in effect on the vaginal epithelium or the endometrium or in the incidence of irregular bleeding. The number of women in whom withdrawal bleeding was reported was considerably reduced by lowering the dosage to 0.2 mg equilin sulphate. Consequently 0.2 to 0.3 mg equilin sulphate must be considered the optimum dosage."}
{"id": "PMID:1094746", "title": "[Intensive care therapy of newborn infants (author's transl)].", "content": "Intensive care of newborn babies consists of immediate resuscitation during the very first minutes after birth, intensive management during the transportation and treatment at the intensive care unit. The importance of immediate resuscitation for the following therapeutic management is stressed. The modern system of correct and well-organized transport for newborn babies is also described. As to the diagnostic and therapeutic management in the intensive care unit, attention is called to the fact that it does not consist only of artificial respiration; in particular, temperature, blood glucose concentration, blood gas analysis and all vital functions must be constantly controlled. The importance of exact dosage of the administered oxygen during the intensive care of newborn babies is demonstrated. The indications, practical management and complications of artificial respiration are detailed. Finally, the good results which can be achieved in this field, with a significant reduction in mortality of artificially-respirated infants, are shown by the present investigation. Good organization, modern technical equipment and, especially, well-trained staff are indispensable foundations for the successful management of newborn infants requiring intensive care.", "contents": "[Intensive care therapy of newborn infants (author's transl)]. Intensive care of newborn babies consists of immediate resuscitation during the very first minutes after birth, intensive management during the transportation and treatment at the intensive care unit. The importance of immediate resuscitation for the following therapeutic management is stressed. The modern system of correct and well-organized transport for newborn babies is also described. As to the diagnostic and therapeutic management in the intensive care unit, attention is called to the fact that it does not consist only of artificial respiration; in particular, temperature, blood glucose concentration, blood gas analysis and all vital functions must be constantly controlled. The importance of exact dosage of the administered oxygen during the intensive care of newborn babies is demonstrated. The indications, practical management and complications of artificial respiration are detailed. Finally, the good results which can be achieved in this field, with a significant reduction in mortality of artificially-respirated infants, are shown by the present investigation. Good organization, modern technical equipment and, especially, well-trained staff are indispensable foundations for the successful management of newborn infants requiring intensive care."}
{"id": "PMID:1094747", "title": "[Clinical and immunological studies in idiopathic adrenal insufficiency].", "content": "The application of 2 special methods to the pathogenetic elucidation of idiopathic Addison's disease is discussed in the light of a clinical case. In accordance with the literature, this patient with idiopathic Addison's disease showed a normal adrenomedullary response (as gauged by the catecholamine excretion and the increase in plasma renin activity) to 2-deoxy-D-glucose administration. Moreover, IgG antibodies were demonstrable in the patient's serum with respect to adrenocortical antigens of the cytoplasmic components of the endocrine cells of the zona glomerulosa and zona fisciculata.", "contents": "[Clinical and immunological studies in idiopathic adrenal insufficiency]. The application of 2 special methods to the pathogenetic elucidation of idiopathic Addison's disease is discussed in the light of a clinical case. In accordance with the literature, this patient with idiopathic Addison's disease showed a normal adrenomedullary response (as gauged by the catecholamine excretion and the increase in plasma renin activity) to 2-deoxy-D-glucose administration. Moreover, IgG antibodies were demonstrable in the patient's serum with respect to adrenocortical antigens of the cytoplasmic components of the endocrine cells of the zona glomerulosa and zona fisciculata."}
{"id": "PMID:1094748", "title": "[Clinical pharmacology of Opino-gel--the demonstration of its effectiveness (author's transl)].", "content": "The effectiveness of topically-applied buphenin (in a gel basis) was demonstrated by rheography in a double-blind study. This finding was additionally corroborated by experimental local vascular (venous) rheography. The flow volume of the tested vessels showed an improvement.", "contents": "[Clinical pharmacology of Opino-gel--the demonstration of its effectiveness (author's transl)]. The effectiveness of topically-applied buphenin (in a gel basis) was demonstrated by rheography in a double-blind study. This finding was additionally corroborated by experimental local vascular (venous) rheography. The flow volume of the tested vessels showed an improvement."}
{"id": "PMID:1094759", "title": "[Results of surgical management of scoliosis and kyphoscoliosis in adults (author's transl)].", "content": "Report about 60 operated scoliosis and kyphoscoliosis experienced at adults above 18 years. After separating the patients into male and female groups, age and localisation the most severe preoperative findings which make the operative treatment more difficult, will be described. Much impact will be put on pre-operative respiratoric and cardial impairment. The evaluation of scoliosis in adults will be proofed by examples. The operative technique, whose basis is the Harrington instrumentation in the modification by Stagnara, will be described also. 72 operative treatments have been necessary at 60 patients. In many cases rip-resections and dorsal osteotomies of the spine have been done. In oposition to the correction results of 46.2% on scoliosis and 56.5% on kyphoscoliosis there is one pulmonary embolism ending deadly and one irreversible paraplegia.", "contents": "[Results of surgical management of scoliosis and kyphoscoliosis in adults (author's transl)]. Report about 60 operated scoliosis and kyphoscoliosis experienced at adults above 18 years. After separating the patients into male and female groups, age and localisation the most severe preoperative findings which make the operative treatment more difficult, will be described. Much impact will be put on pre-operative respiratoric and cardial impairment. The evaluation of scoliosis in adults will be proofed by examples. The operative technique, whose basis is the Harrington instrumentation in the modification by Stagnara, will be described also. 72 operative treatments have been necessary at 60 patients. In many cases rip-resections and dorsal osteotomies of the spine have been done. In oposition to the correction results of 46.2% on scoliosis and 56.5% on kyphoscoliosis there is one pulmonary embolism ending deadly and one irreversible paraplegia."}
{"id": "PMID:1094760", "title": "[Investigations of contact tissues of Blount vitallium staples using the instrumental neutron activation analysis (author's transl)].", "content": "Instrumental neutron activation analysis with Gamma-spectroscopy is a very sensitive method for testing trace elements. Specimens of contact tissue around 5 Blount's vitallium clamps were, after preparation at the Nuclear Research Institute J\u00fclich irradiated with thermic neutrons and their element-specific gamma spectra were measured after 33 and 125 days. In contact tissue a significant rise in concentration of the alloy-specific elements cobalt and chromium was found. The unspecific elements silver, caesium, iron, rubidium, antimon, scandium and zink were either within or very close to the normal. The metallosis of contact tissue is due to corrosion of the surfaces of cobalt-chromium implants.", "contents": "[Investigations of contact tissues of Blount vitallium staples using the instrumental neutron activation analysis (author's transl)]. Instrumental neutron activation analysis with Gamma-spectroscopy is a very sensitive method for testing trace elements. Specimens of contact tissue around 5 Blount's vitallium clamps were, after preparation at the Nuclear Research Institute J\u00fclich irradiated with thermic neutrons and their element-specific gamma spectra were measured after 33 and 125 days. In contact tissue a significant rise in concentration of the alloy-specific elements cobalt and chromium was found. The unspecific elements silver, caesium, iron, rubidium, antimon, scandium and zink were either within or very close to the normal. The metallosis of contact tissue is due to corrosion of the surfaces of cobalt-chromium implants."}
{"id": "PMID:1094764", "title": "[Electron-microscopic demonstration of virus-like structures in the synovial membrane in chronic progressive polyarthritis].", "content": "The recent demonstration of ultramicroscopic \"virus-like\" cell inclusions in various organs in patients with collagen and autoimmune diseases suggest that viruses may play an aetiological role in these diseases. This paper reports briefly on some of the evidence for such \"virus-like\" structures in different cells of the synovial membrane from two patients with rheumatoid arthritis. The inclusions were localised in the vaculoes of the endoplasmatic reticulum of endothelial cells, lymphocytes, macrophages, fibroblasts and perithelial cells. They appear as tubular bodies of approximately 250 A. diameter. The tubules join together so that they form a 3-dimensional network. The possible viral or non-viral nature of these inclusions is discussed. A ultramicroscopical resemblance to the RNA-containing paramyxo-viruses suggests their virus nature. However, the negative results in testing with serum antibodies and immunofluorescence techniques argue against this. The authors suggest that the demonstration of these inclusions in other than collagen diseases points more in the direction of the correlation of these inclusions with immune globulin synthesis rather than the presence of a virus.", "contents": "[Electron-microscopic demonstration of virus-like structures in the synovial membrane in chronic progressive polyarthritis]. The recent demonstration of ultramicroscopic \"virus-like\" cell inclusions in various organs in patients with collagen and autoimmune diseases suggest that viruses may play an aetiological role in these diseases. This paper reports briefly on some of the evidence for such \"virus-like\" structures in different cells of the synovial membrane from two patients with rheumatoid arthritis. The inclusions were localised in the vaculoes of the endoplasmatic reticulum of endothelial cells, lymphocytes, macrophages, fibroblasts and perithelial cells. They appear as tubular bodies of approximately 250 A. diameter. The tubules join together so that they form a 3-dimensional network. The possible viral or non-viral nature of these inclusions is discussed. A ultramicroscopical resemblance to the RNA-containing paramyxo-viruses suggests their virus nature. However, the negative results in testing with serum antibodies and immunofluorescence techniques argue against this. The authors suggest that the demonstration of these inclusions in other than collagen diseases points more in the direction of the correlation of these inclusions with immune globulin synthesis rather than the presence of a virus."}
{"id": "PMID:1094767", "title": "[Effect on quinoline derivatives, nucleotides, D-penicillamine,cytostatic and antiphogistic drugs on the rheumatoid factors other immune antibodies].", "content": "Cytostatics have no effect on antigen-antibody reactions in vitro. Immunosuppression by cytostatics is mainly based on the inhibition of immunocytes. Prednisolone inhibits RA-factors in the Waaler-Rose-test; the mechanism of this effect is not understood at the present time. Acetylsalicylic acid has no influence on the antigen-antibody reactions investigated. Nucleotides inhibit only ANA; the effect is probably based on a cross reaction with ANA. D-enicillamine inhibits RA-factors and other IgM-antibodies by splitting SS-bonds in macromolecules of immunoglobulins. Quinoline derivatives inhibit RA-factors, ANA, cold agglutinins and IgG-isoantibodies and autoantibodies to erythrocytes. Contrary to nucleotides, prednisolone and d-penicillamine, which also inhibit antigen-antibody reactions, chloroquine is splitting immune complexes; after the aplitting of the complexes the reactivity of antigens and antibodies is not impaired. The mechanism of action of chloroquine might be connected with physical and chemical processes of the antigen-antibody reaction. The reduction of the surface charge of human red cells is not related to the inhibition of antigen-antibody reactions by the  substances investigated.", "contents": "[Effect on quinoline derivatives, nucleotides, D-penicillamine,cytostatic and antiphogistic drugs on the rheumatoid factors other immune antibodies]. Cytostatics have no effect on antigen-antibody reactions in vitro. Immunosuppression by cytostatics is mainly based on the inhibition of immunocytes. Prednisolone inhibits RA-factors in the Waaler-Rose-test; the mechanism of this effect is not understood at the present time. Acetylsalicylic acid has no influence on the antigen-antibody reactions investigated. Nucleotides inhibit only ANA; the effect is probably based on a cross reaction with ANA. D-enicillamine inhibits RA-factors and other IgM-antibodies by splitting SS-bonds in macromolecules of immunoglobulins. Quinoline derivatives inhibit RA-factors, ANA, cold agglutinins and IgG-isoantibodies and autoantibodies to erythrocytes. Contrary to nucleotides, prednisolone and d-penicillamine, which also inhibit antigen-antibody reactions, chloroquine is splitting immune complexes; after the aplitting of the complexes the reactivity of antigens and antibodies is not impaired. The mechanism of action of chloroquine might be connected with physical and chemical processes of the antigen-antibody reaction. The reduction of the surface charge of human red cells is not related to the inhibition of antigen-antibody reactions by the  substances investigated."}
{"id": "PMID:1094776", "title": "Mixed gonadal dysgenesis.", "content": "Mixed gonadal dysgenesis (MCG) is a rare intersexual disorder, characterized in most cases by the presence of a testis and a contralateral streak gonad; in some cases the contralateral gonad may be rudimentary not having differentiated into an ovary or into a testis and in other cases it may be absent. A personal case was recently studied by the authors in some detail: A 51-year-old individual reared as a female with primary amenorrhea, short stature, subnormal intelligence, male type habitus, hirsutism, moderate breast development, ambiguous external genitalia with a 5 x 2 cm phallus, labia majora with scrotal type skin, and a urogenital sinus. Internal genitalia consisted of a hypoplastic vagina and uterus, a streak gonad on the right and intraabdominal testis, fallopian tube, and epididymis on the left side. The dysgenetic testis contained a gonadoblastoma and the streak a gonadoblastoma and dysgerminoma. A buccal smear was chromatin negative and karyotype analysis revealed XO/XY mosaicism. Thyroid and adrenocortical function was normal. A plasma testosterone level of 0.15 mu-g% rose to 0.45 mu-g% with 3 days of HCG administration. With bilateral gonadectomy the plasma testosterone level fell to 0.06 mu-g% and there was no response following HCG administration. 109 cases of MGD and 2 cases of unilateral gonadal dysgenesis reported in the world literature between 1937 and 1973 were reviewed. On the basis of their clinical and pathological features, the latter two patients were excluded from the category of MGD. In 15 of the 109 cases of MGD, a gonadoblastoma obscuring the gonad of origin was opposed by a dysgenetic gonad with or without a gonadoblastoma or no gonad. In these cases the presence of a testis was not proven histologically and their clinical-pathological features were similar to those found in pure gonadal dysgenesis. Of the remaining 94 cases of MGD, 72 had testicular tissue on one side and a streak or embryonic gonad on the other while 22 had a testis and no contralateral gonad...", "contents": "Mixed gonadal dysgenesis. Mixed gonadal dysgenesis (MCG) is a rare intersexual disorder, characterized in most cases by the presence of a testis and a contralateral streak gonad; in some cases the contralateral gonad may be rudimentary not having differentiated into an ovary or into a testis and in other cases it may be absent. A personal case was recently studied by the authors in some detail: A 51-year-old individual reared as a female with primary amenorrhea, short stature, subnormal intelligence, male type habitus, hirsutism, moderate breast development, ambiguous external genitalia with a 5 x 2 cm phallus, labia majora with scrotal type skin, and a urogenital sinus. Internal genitalia consisted of a hypoplastic vagina and uterus, a streak gonad on the right and intraabdominal testis, fallopian tube, and epididymis on the left side. The dysgenetic testis contained a gonadoblastoma and the streak a gonadoblastoma and dysgerminoma. A buccal smear was chromatin negative and karyotype analysis revealed XO/XY mosaicism. Thyroid and adrenocortical function was normal. A plasma testosterone level of 0.15 mu-g% rose to 0.45 mu-g% with 3 days of HCG administration. With bilateral gonadectomy the plasma testosterone level fell to 0.06 mu-g% and there was no response following HCG administration. 109 cases of MGD and 2 cases of unilateral gonadal dysgenesis reported in the world literature between 1937 and 1973 were reviewed. On the basis of their clinical and pathological features, the latter two patients were excluded from the category of MGD. In 15 of the 109 cases of MGD, a gonadoblastoma obscuring the gonad of origin was opposed by a dysgenetic gonad with or without a gonadoblastoma or no gonad. In these cases the presence of a testis was not proven histologically and their clinical-pathological features were similar to those found in pure gonadal dysgenesis. Of the remaining 94 cases of MGD, 72 had testicular tissue on one side and a streak or embryonic gonad on the other while 22 had a testis and no contralateral gonad..."}
{"id": "PMID:1094777", "title": "[Choice of recipent in human cardiac transplantation].", "content": "The favourable clinical results of cardiac transplantation merit wider application of this therapeutic measure in the management of non-obstructive primary cardiomyopathies and diffuse myocardial fibrosis secondary to coronary artery disease and in its terminal phase, in the absence of any absolute contraindication (pulmonary resistance too high, diabetes, gastrointestinal disorder, infection, patient too old.). Close cooperation between departments of cardiology and surgical transplantation centres, with systematic study of the patients pre-operatively and the setting up of a waiting list of recipients, will make possible in the future the improvement of the already encouraging results of cardiac transplantation.", "contents": "[Choice of recipent in human cardiac transplantation]. The favourable clinical results of cardiac transplantation merit wider application of this therapeutic measure in the management of non-obstructive primary cardiomyopathies and diffuse myocardial fibrosis secondary to coronary artery disease and in its terminal phase, in the absence of any absolute contraindication (pulmonary resistance too high, diabetes, gastrointestinal disorder, infection, patient too old.). Close cooperation between departments of cardiology and surgical transplantation centres, with systematic study of the patients pre-operatively and the setting up of a waiting list of recipients, will make possible in the future the improvement of the already encouraging results of cardiac transplantation."}
{"id": "PMID:1094781", "title": "High-frequency positive-pressure ventilation (hfppv) during transthoracic resection of tracheal stenosis and during peroperative bronchoscopic examination.", "content": "Operation of a patient with intrathoracic tracheal stenosis using a new ventilation technique (HFPPV) is described. The technique permits tracheoscopy during ventilation and operation, thus enabling exact location of the stenosis to be obtained. Further, peroperative tracheoscopic checking of the anastomosis can be carried out. Resection and anastomosis can be performed without interference of a bulky endotracheal tube.", "contents": "High-frequency positive-pressure ventilation (hfppv) during transthoracic resection of tracheal stenosis and during peroperative bronchoscopic examination. Operation of a patient with intrathoracic tracheal stenosis using a new ventilation technique (HFPPV) is described. The technique permits tracheoscopy during ventilation and operation, thus enabling exact location of the stenosis to be obtained. Further, peroperative tracheoscopic checking of the anastomosis can be carried out. Resection and anastomosis can be performed without interference of a bulky endotracheal tube."}
{"id": "PMID:1094782", "title": "Effects of different inspiratory gas flow patterns on thoracic compliance during respirator treatment.", "content": "Nine patients under general anaesthesia and 26 intensive care patients undergoing respirator treatment were investigated with special reference to changes in compliance under the influence of different inspiratory gas flow patterns during intermittent positive pressure ventilation (IPPV). Pressure recordings were performed in the respirator tubings, in the trachea and in the oesophagus. Flow rate was measured with pneumotachography. Total compliance showed an increase with a decelerating flow compared to an accelerating flow in the anaesthetized group as well as in patients with low compliance and high resistance, and also in patients with chronic respiratory disease. These findings were explained by an inproved evenness in the distribution of gas in the greater airways during the inspiratory phase. A separation of total compliance into lung and chest wall compliance did not reveal any significant variation correlated to flow pattern. The change in compliance occurred within minutes and remained during a 30-minute setting period.", "contents": "Effects of different inspiratory gas flow patterns on thoracic compliance during respirator treatment. Nine patients under general anaesthesia and 26 intensive care patients undergoing respirator treatment were investigated with special reference to changes in compliance under the influence of different inspiratory gas flow patterns during intermittent positive pressure ventilation (IPPV). Pressure recordings were performed in the respirator tubings, in the trachea and in the oesophagus. Flow rate was measured with pneumotachography. Total compliance showed an increase with a decelerating flow compared to an accelerating flow in the anaesthetized group as well as in patients with low compliance and high resistance, and also in patients with chronic respiratory disease. These findings were explained by an inproved evenness in the distribution of gas in the greater airways during the inspiratory phase. A separation of total compliance into lung and chest wall compliance did not reveal any significant variation correlated to flow pattern. The change in compliance occurred within minutes and remained during a 30-minute setting period."}
{"id": "PMID:1094783", "title": "Effects of different gas flow patterns on central circulation during respirator treatment.", "content": "The effects of different inspiratory gas flow patterns on the central circulation during respirator treatment were investigated, first with a retrospective study consisting of 15 patients, where the effects on the mean intrathoracic pressure were represented by the variations in the amplitude and time development of the oesophageal pressure curve. In another study on eight intensive care patients undergoing respirator treatment, right-sided heart catheterisation was performed with pressure recordings in the central venous position, the right ventricle, the pulmonary artery and in the pulmonary capillary venous position. Estimations of the arterial-mixed venous oxygen content difference were also made. The development and transmission of the airway pressure curve to the central circulation were illustrated and discussed. The results showed very small effects on the mean intrathoracic pressure from different inspiratory gas flow patterns, and, therefore, it could be expected that the effects on the central circulation would be insignificant. This was also the conclusion in the catheterisation study. No important changes in the pressures of the a-vo2 difference occured. Most of the characteristics of a particular gas flow pattern are lost during the passage through the airways and are not transmitted to the oesophagus or the central vessels.", "contents": "Effects of different gas flow patterns on central circulation during respirator treatment. The effects of different inspiratory gas flow patterns on the central circulation during respirator treatment were investigated, first with a retrospective study consisting of 15 patients, where the effects on the mean intrathoracic pressure were represented by the variations in the amplitude and time development of the oesophageal pressure curve. In another study on eight intensive care patients undergoing respirator treatment, right-sided heart catheterisation was performed with pressure recordings in the central venous position, the right ventricle, the pulmonary artery and in the pulmonary capillary venous position. Estimations of the arterial-mixed venous oxygen content difference were also made. The development and transmission of the airway pressure curve to the central circulation were illustrated and discussed. The results showed very small effects on the mean intrathoracic pressure from different inspiratory gas flow patterns, and, therefore, it could be expected that the effects on the central circulation would be insignificant. This was also the conclusion in the catheterisation study. No important changes in the pressures of the a-vo2 difference occured. Most of the characteristics of a particular gas flow pattern are lost during the passage through the airways and are not transmitted to the oesophagus or the central vessels."}
{"id": "PMID:1094786", "title": "Blood-brain barrier lesions in acute hypertension in rabbits after unilateral X-ray exposure of brain.", "content": "Acute hypertension was induced by metaraminol at different time intervals following irradiation in rabbits exposed to unilateral X-ray irradiation against the brain (3000 R) and in control animals. Hypertension resulted in a few areas of Evans blue extravasation in control animals. In the irradiated animals there was a marked increase in tracer extravasation on the half of the brain exposed to X-rays, indicating an increased vulnerability of cerebral vessels to blood pressure increase after irradiation.", "contents": "Blood-brain barrier lesions in acute hypertension in rabbits after unilateral X-ray exposure of brain. Acute hypertension was induced by metaraminol at different time intervals following irradiation in rabbits exposed to unilateral X-ray irradiation against the brain (3000 R) and in control animals. Hypertension resulted in a few areas of Evans blue extravasation in control animals. In the irradiated animals there was a marked increase in tracer extravasation on the half of the brain exposed to X-rays, indicating an increased vulnerability of cerebral vessels to blood pressure increase after irradiation."}
{"id": "PMID:1094787", "title": "Treatment of imminent premature labour.", "content": "In this study, 194 imminent premature deliveries were treated. The pregnancies were in the 28th to 36th week. Uterine contractions were demonstrable in all patients and amniotic membranes were intact. All patients were treated with bedrest. Two betasympathomimetics were used in a double-blind study: Nyldrin hydrochloride (43 cases) and Isoxuprine hydrochloride (60 cases). A placebo was given to 41 patients, and ethyl alcohol to 50 patients. Intravenous and intramuscular treatment given in the hospital was continued with oral administration at home, and follow-up examinations were repeated at short intervals. Taking a minimum birth weight of 2500 g as the criteria of successful treatment, the success rate in the placebo group was 71%, in the Nylidrin hydrochloride group 86%, the Isoxuprine hydrochloride group 75% and the alcohol group 70%. When premature delivery was postponed 7 days, the pregnancy advanced., to the 37th week or later in 73, 77, 62 and 56% in their respective groups. The beta-sympathomimetics, especially the Nylidrin hydrochloride, were in every respect more efficient than placebo or alcohol. The therapeutic effect of alcohol was no better than that obtained with placebo. From the fetal point of view, the drugs used in the present study showed no adverse-effects.", "contents": "Treatment of imminent premature labour. In this study, 194 imminent premature deliveries were treated. The pregnancies were in the 28th to 36th week. Uterine contractions were demonstrable in all patients and amniotic membranes were intact. All patients were treated with bedrest. Two betasympathomimetics were used in a double-blind study: Nyldrin hydrochloride (43 cases) and Isoxuprine hydrochloride (60 cases). A placebo was given to 41 patients, and ethyl alcohol to 50 patients. Intravenous and intramuscular treatment given in the hospital was continued with oral administration at home, and follow-up examinations were repeated at short intervals. Taking a minimum birth weight of 2500 g as the criteria of successful treatment, the success rate in the placebo group was 71%, in the Nylidrin hydrochloride group 86%, the Isoxuprine hydrochloride group 75% and the alcohol group 70%. When premature delivery was postponed 7 days, the pregnancy advanced., to the 37th week or later in 73, 77, 62 and 56% in their respective groups. The beta-sympathomimetics, especially the Nylidrin hydrochloride, were in every respect more efficient than placebo or alcohol. The therapeutic effect of alcohol was no better than that obtained with placebo. From the fetal point of view, the drugs used in the present study showed no adverse-effects."}
{"id": "PMID:1094788", "title": "Retrograde degeneration of the cochlear nerve.", "content": "Retrograde degeneration of the cochlear neurons has been studied in different types and degrees of peripheral cochlear damage such as acoustic trauma, intoxication, heredodegenerative deafness and others. It starts only when the peripheral dendrites to the inner hair cells are irreversibly damaged. About 10% of the neurons are not affected by retrograde degeneration. They correspond to the type II and III neurons, which also survive after transection of the cochlear nerve and are mainly associated with the outer hair cells. Cochlear damage due to vascular impairment usually leads to a complete loss of cochlear neurons. In hereditary abiotrophic deafness, neuronal degeneration is slower and its extent varies considerably according to the various genetic syndromes.", "contents": "Retrograde degeneration of the cochlear nerve. Retrograde degeneration of the cochlear neurons has been studied in different types and degrees of peripheral cochlear damage such as acoustic trauma, intoxication, heredodegenerative deafness and others. It starts only when the peripheral dendrites to the inner hair cells are irreversibly damaged. About 10% of the neurons are not affected by retrograde degeneration. They correspond to the type II and III neurons, which also survive after transection of the cochlear nerve and are mainly associated with the outer hair cells. Cochlear damage due to vascular impairment usually leads to a complete loss of cochlear neurons. In hereditary abiotrophic deafness, neuronal degeneration is slower and its extent varies considerably according to the various genetic syndromes."}
{"id": "PMID:1094798", "title": "Cellular sites of immunoglobulins . VI. Localization of immunoglobulins in the human thymus.", "content": "IgG-, IgM and IgA-containing cells in the human thymus and mesenteric lymph nodes were studied by direct, double-antibody immunofluorescence techniques. Ig-containing cells were identified in the thymic medulla but not in cortical or paracortical zones. About 93 percent of the medullary Ig-containing cells were IgG-containing (vs. 5 percent IgM containing and 2 percent IgA - containing). By contrast, lymph nodes contained about 53 percent IgG, 31 percent IgA and 16 percent IgM type immunocytes. In addition, Ig-containing cells were about one-tenth as numerous in thymus as in lymph nodes.", "contents": "Cellular sites of immunoglobulins . VI. Localization of immunoglobulins in the human thymus. IgG-, IgM and IgA-containing cells in the human thymus and mesenteric lymph nodes were studied by direct, double-antibody immunofluorescence techniques. Ig-containing cells were identified in the thymic medulla but not in cortical or paracortical zones. About 93 percent of the medullary Ig-containing cells were IgG-containing (vs. 5 percent IgM containing and 2 percent IgA - containing). By contrast, lymph nodes contained about 53 percent IgG, 31 percent IgA and 16 percent IgM type immunocytes. In addition, Ig-containing cells were about one-tenth as numerous in thymus as in lymph nodes."}
{"id": "PMID:1094807", "title": "Involution of polycystic kidneys during active treatment of terminal uremia.", "content": "Following institution of chronic dialysis and/or renal transplantation for terminal uremia, four consecutive patients observed for 15-48 months with their polycystic kidneys in situ invariably showed a rapid relief of symptoms (pain, hematuria) followed by a gradual, but steady, involution of the kidneys. The mechanism and the practical clinical implications are discussed.", "contents": "Involution of polycystic kidneys during active treatment of terminal uremia. Following institution of chronic dialysis and/or renal transplantation for terminal uremia, four consecutive patients observed for 15-48 months with their polycystic kidneys in situ invariably showed a rapid relief of symptoms (pain, hematuria) followed by a gradual, but steady, involution of the kidneys. The mechanism and the practical clinical implications are discussed."}
{"id": "PMID:1094819", "title": "Comparative hemodynamic effects of placebo and oral isosorbide dinitrate in patients with significant coronary artery disease.", "content": "Comparative hemodynamic effects of placebo and 10 mg of oral isosorbide dinitrate were studied in patients with significant coronary artery disease (larger than or equal to 75 per cent lumen narrowing) proved angiographically. Isosorbide dinitrate or placebo was given to eight and 10 patients, respectively, in a double-blind fashion. Cardiac performance at rest and during supine leg exercise was evaluated before and 60 minutes after drug administration. In the resting state, isosorbide dinitrate compared to placebo significantly reduced the left ventricualr (systolic and diastolic), mean pulmonary artery and mean aortic pressures, cardiac index, stroke index, left ventricular work index, stroke work index, and mean systolic ejection rate. Isosorbide dinitrate also significantly reduced left ventricular (systolic and diastolic and mean pulmonary artery pressures during exercise. This study indicates that 10 mg of isosorbide dinitrate has a significant influence on ischemic left ventricular dysfunction 60 minutes after its oral administration.", "contents": "Comparative hemodynamic effects of placebo and oral isosorbide dinitrate in patients with significant coronary artery disease. Comparative hemodynamic effects of placebo and 10 mg of oral isosorbide dinitrate were studied in patients with significant coronary artery disease (larger than or equal to 75 per cent lumen narrowing) proved angiographically. Isosorbide dinitrate or placebo was given to eight and 10 patients, respectively, in a double-blind fashion. Cardiac performance at rest and during supine leg exercise was evaluated before and 60 minutes after drug administration. In the resting state, isosorbide dinitrate compared to placebo significantly reduced the left ventricualr (systolic and diastolic), mean pulmonary artery and mean aortic pressures, cardiac index, stroke index, left ventricular work index, stroke work index, and mean systolic ejection rate. Isosorbide dinitrate also significantly reduced left ventricular (systolic and diastolic and mean pulmonary artery pressures during exercise. This study indicates that 10 mg of isosorbide dinitrate has a significant influence on ischemic left ventricular dysfunction 60 minutes after its oral administration."}
{"id": "PMID:1094825", "title": "Review of computer applications in hospital pharmacy practice.", "content": "A comprehensive literature review of the applications of computers in hospital pharmacy practice is presented. Articles are categorized as: accounting and drug usage review; purchasing and inventory control; controlled substances; outpatient services;drug distribution; drug information; and clinical services.", "contents": "Review of computer applications in hospital pharmacy practice. A comprehensive literature review of the applications of computers in hospital pharmacy practice is presented. Articles are categorized as: accounting and drug usage review; purchasing and inventory control; controlled substances; outpatient services;drug distribution; drug information; and clinical services."}
{"id": "PMID:1094826", "title": "Adolescent hypertension.", "content": "The present appraisal indicates that blood pressure patterns in adolescence differ from those in adulthood and that the design of hypertension detection programs needs to be modified for this age group. Routine blood pressure screening within the school system would appear to be the most effective means for early detection of hypertension. Links with established hypertension clinics, preferably within an adolescent unit, should be forged so that health preservation becomes a meaningful part of the educational process. Although primary hypertension has been documented in the adolescent age group, the incidence is unknown and careful diagnostic evaluation is warranted until our knowledge of its characteristics is more fully expanded.", "contents": "Adolescent hypertension. The present appraisal indicates that blood pressure patterns in adolescence differ from those in adulthood and that the design of hypertension detection programs needs to be modified for this age group. Routine blood pressure screening within the school system would appear to be the most effective means for early detection of hypertension. Links with established hypertension clinics, preferably within an adolescent unit, should be forged so that health preservation becomes a meaningful part of the educational process. Although primary hypertension has been documented in the adolescent age group, the incidence is unknown and careful diagnostic evaluation is warranted until our knowledge of its characteristics is more fully expanded."}
{"id": "PMID:1094827", "title": "Electron dense alteration of kidney basement membranes. A renal lesion specific of a systemic disease.", "content": "Described here are the findings of a clinical, anatomic and chemical study of 40 patients having an original lesion of the basement membranes of the kidney. This lesion is characterized by an ultrastructural modification of the basement membrane substance which appears very dark (black) and homogeneous in electron micrographs. It affects the basement membranes of the glomerulus, Bowman's capsule and the renal tubules. Patients with this lesion all manifest the clinical course of a chronic enphritis and is almost invariably accompanied by proteinuria and microscopic hematuria. The disease evolves generally with intermittent episodes towards irreversible renal failure. Eight patients received kidney transplants. Study of these transplants has shown that the lesion recurs in the transplanted kidneys during the year following transplantation. Moreover, it is never found in kidneys transplanted into patients who did not have it in their own kidneys. This ultrastructural lesion can therefore be placed in the framework of a systemic disease whose etiology is not yet known. The study of these transplants also shows that the lesion can exist despite the absence of any detectable clinical or biologic signs and be well tolerated for periods which now exceed 4 years for three patients.", "contents": "Electron dense alteration of kidney basement membranes. A renal lesion specific of a systemic disease. Described here are the findings of a clinical, anatomic and chemical study of 40 patients having an original lesion of the basement membranes of the kidney. This lesion is characterized by an ultrastructural modification of the basement membrane substance which appears very dark (black) and homogeneous in electron micrographs. It affects the basement membranes of the glomerulus, Bowman's capsule and the renal tubules. Patients with this lesion all manifest the clinical course of a chronic enphritis and is almost invariably accompanied by proteinuria and microscopic hematuria. The disease evolves generally with intermittent episodes towards irreversible renal failure. Eight patients received kidney transplants. Study of these transplants has shown that the lesion recurs in the transplanted kidneys during the year following transplantation. Moreover, it is never found in kidneys transplanted into patients who did not have it in their own kidneys. This ultrastructural lesion can therefore be placed in the framework of a systemic disease whose etiology is not yet known. The study of these transplants also shows that the lesion can exist despite the absence of any detectable clinical or biologic signs and be well tolerated for periods which now exceed 4 years for three patients."}
{"id": "PMID:1094828", "title": "Alternative C3 pathway activiation in pneumococcal glomerulonephritis.", "content": "Glomerulonephritis following pneumococcal infection has been observed, but possible immunopathologic mechanisms have not been adequately explored. Multiple serologic studies as well as light, immunofluorescence and electron microscopic evaluation of kidney biopsy tissue from a 4 year old girl with pneumococcal glomerulonephritis were performed. Clinical studies at the onset of the disease showed normal serum C3 and C4 levels (third and fourth components of complement) with progression to selective C3 hypocomplementemia from days 2 to 58. A serum factor capable of breaking down C3 in normal human serum was present during the period of maximum C3 hypocomplementemia. Renal glomerular histology revealed a mesangial proliferative glomerulonephritis. Glomerular bound C3 and type 14 pneumococcal antigen were associated with similar, but less extensive, deposits of properdin. Minimal immunoglobulin M (IgM) and C4 were seen, but immunoglobulin G (IgG) and fibrinogen were absent. Ultrastructurally, subepithelial \"humps\" and intramembranous electron dense deposits were noted. It is hypothesized that the pneumococcal polysaccharide can activate the alternate complement pathway and may be responsible for a limited course of glomerulonephritis.", "contents": "Alternative C3 pathway activiation in pneumococcal glomerulonephritis. Glomerulonephritis following pneumococcal infection has been observed, but possible immunopathologic mechanisms have not been adequately explored. Multiple serologic studies as well as light, immunofluorescence and electron microscopic evaluation of kidney biopsy tissue from a 4 year old girl with pneumococcal glomerulonephritis were performed. Clinical studies at the onset of the disease showed normal serum C3 and C4 levels (third and fourth components of complement) with progression to selective C3 hypocomplementemia from days 2 to 58. A serum factor capable of breaking down C3 in normal human serum was present during the period of maximum C3 hypocomplementemia. Renal glomerular histology revealed a mesangial proliferative glomerulonephritis. Glomerular bound C3 and type 14 pneumococcal antigen were associated with similar, but less extensive, deposits of properdin. Minimal immunoglobulin M (IgM) and C4 were seen, but immunoglobulin G (IgG) and fibrinogen were absent. Ultrastructurally, subepithelial \"humps\" and intramembranous electron dense deposits were noted. It is hypothesized that the pneumococcal polysaccharide can activate the alternate complement pathway and may be responsible for a limited course of glomerulonephritis."}
{"id": "PMID:1094829", "title": "Meningococcal antigen in diagnosis and treatment of group A meningococcal infections.", "content": "Meningococcal antigen was measured by countercurrent immunoelectrophoresis in the blood and cerebrospinal fluid of 200 patients with group A meningococcal meningitis. Antigen was detected in the blood of 27 (13.5 per cent) patients. These patients had a worse prognosis and a higher incidence of allergic complications, such as arthritis and vasculitis, about 5 days after the start of antibiotic treatment. Antigen was found in the CSF of 129 (67.5 per cent) patients); antigen often persisted in the cerebrospinal fluid despite antibiotic treatment before admission. A combination of immunoelectrophoresis and routine bacteriologic study was used in the diagnosis of 162 (84.8 per cent) patients with meningococcal meningitis. High levels of antigen and a slow antigen disappearance were associated with neurologic damage. The antigen is stable and may be detected from specimens of cerebrospinal fluid dried on filter paper.", "contents": "Meningococcal antigen in diagnosis and treatment of group A meningococcal infections. Meningococcal antigen was measured by countercurrent immunoelectrophoresis in the blood and cerebrospinal fluid of 200 patients with group A meningococcal meningitis. Antigen was detected in the blood of 27 (13.5 per cent) patients. These patients had a worse prognosis and a higher incidence of allergic complications, such as arthritis and vasculitis, about 5 days after the start of antibiotic treatment. Antigen was found in the CSF of 129 (67.5 per cent) patients); antigen often persisted in the cerebrospinal fluid despite antibiotic treatment before admission. A combination of immunoelectrophoresis and routine bacteriologic study was used in the diagnosis of 162 (84.8 per cent) patients with meningococcal meningitis. High levels of antigen and a slow antigen disappearance were associated with neurologic damage. The antigen is stable and may be detected from specimens of cerebrospinal fluid dried on filter paper."}
{"id": "PMID:1094830", "title": "Microangiopathic hemolytic anemia in renal allotransplantation. Repart of a successfully treated case and review of the literature.", "content": "The development of microangiopathic hemolytic anemia after renal transplantation in a 17 year old white boy is reported, and the literature is reviewed. In this patient microangiopathic hemolytic anemia developed 6 weeks after renal transplantation during a second episode of rejection. Light, fluorescence and electron microscopy demonstrated the renal vascular lesion associated with this syndrome. In contrast to the other four previously reported cases of microangiopathic hemolytic anemia associated with renal allotransplantation, this patient had complete resolution of the microangiopathic hemolytic anemia with heparin therapy and improved allograft function, presumably with diminution of the vascular lesion. He survived a complicated early period after renal transplantation and has shown no recurrence of microangiopathic hemolytic anemia in the 18 months since transplantation. Special red blood cell and fibrinogen studies are discussed.", "contents": "Microangiopathic hemolytic anemia in renal allotransplantation. Repart of a successfully treated case and review of the literature. The development of microangiopathic hemolytic anemia after renal transplantation in a 17 year old white boy is reported, and the literature is reviewed. In this patient microangiopathic hemolytic anemia developed 6 weeks after renal transplantation during a second episode of rejection. Light, fluorescence and electron microscopy demonstrated the renal vascular lesion associated with this syndrome. In contrast to the other four previously reported cases of microangiopathic hemolytic anemia associated with renal allotransplantation, this patient had complete resolution of the microangiopathic hemolytic anemia with heparin therapy and improved allograft function, presumably with diminution of the vascular lesion. He survived a complicated early period after renal transplantation and has shown no recurrence of microangiopathic hemolytic anemia in the 18 months since transplantation. Special red blood cell and fibrinogen studies are discussed."}
{"id": "PMID:1094832", "title": "Complications of keratoplasty.", "content": "The complications of keratoplasty have been greatly reduced in recent years by virtue of finer instrumentation, microsurgery, new drugs to minimize postoperative complications, and improved methods of donor cornea preversation. A 49-year-old woman, following uncomplicated keratoplasty with donor material from a patient who had died of a slow virus disease, developed the same condition and died 18 months later. Until more evidence is available, donor material from individuals dying of obscure chronic central nervous system disorders should be used with extreme caution.", "contents": "Complications of keratoplasty. The complications of keratoplasty have been greatly reduced in recent years by virtue of finer instrumentation, microsurgery, new drugs to minimize postoperative complications, and improved methods of donor cornea preversation. A 49-year-old woman, following uncomplicated keratoplasty with donor material from a patient who had died of a slow virus disease, developed the same condition and died 18 months later. Until more evidence is available, donor material from individuals dying of obscure chronic central nervous system disorders should be used with extreme caution."}
{"id": "PMID:1094833", "title": "Geometric analysis of radial buckling.", "content": "Tension analysis along the retinal surface demonstrates the advantage of meridional buckles over limbal-parallel buckles. A cylindrical sponge generates tensional forces in the retina along the long axis of the buckle and perpendicular to it. The tension perpendicular to the sponge is positive, and the retina and retinal pigment epithelium stretch in that direction. The tension along the long axis is positive (stretching) when the buckle is less than 90 degrees arc length; and negative (compression), greater than 90 degrees for a 5-mm sponge. Consequently, retinal compression folds on the buckles occur for buckles of arc length greater than 90 degrees. These tension forces at right angles to each other explain the development of (1) concave retinal detachment or retinal folds off short limbal-parallel buckles, (2) retinal folds on long limbal-parallel buckles, and (3) fishmouthing of horseshoe tears overlying a limbal-parallel buckle. The buckle increases retinal surface area overlying the buckle. The significance of this is not clear, but it may explain the lessening or disappearance of compression folds on the buckle in time.", "contents": "Geometric analysis of radial buckling. Tension analysis along the retinal surface demonstrates the advantage of meridional buckles over limbal-parallel buckles. A cylindrical sponge generates tensional forces in the retina along the long axis of the buckle and perpendicular to it. The tension perpendicular to the sponge is positive, and the retina and retinal pigment epithelium stretch in that direction. The tension along the long axis is positive (stretching) when the buckle is less than 90 degrees arc length; and negative (compression), greater than 90 degrees for a 5-mm sponge. Consequently, retinal compression folds on the buckles occur for buckles of arc length greater than 90 degrees. These tension forces at right angles to each other explain the development of (1) concave retinal detachment or retinal folds off short limbal-parallel buckles, (2) retinal folds on long limbal-parallel buckles, and (3) fishmouthing of horseshoe tears overlying a limbal-parallel buckle. The buckle increases retinal surface area overlying the buckle. The significance of this is not clear, but it may explain the lessening or disappearance of compression folds on the buckle in time."}
{"id": "PMID:1094837", "title": "Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline-deficient diet.", "content": "Female, albino mice were fed a choline-deficient diet containing 0.5% DL-ethionine. All animals died within 5 days due to the development of an acute hemorrhagic pancreatis with fat necrosis throughout the peritoneal cavity. The apancreatitis was characterized by a massive necrosis of the exocrine parenchyma with intense hemorrhage and inflammatory reaction of the stroma. The sequence of histologic and ultrastructural alterations occurring in the acinar cells of the pancreas were studied in mice fed the diet for 1, 2, and 3 days. Major findings consited of accumulation of zymogen granules, vacuolation due to foci of cytoplasmic degradation, and alterations in the morphology of the zymogen granules. The pancreatitis appears to be due to intraparenchymal activation of zymogens, resulting from a synergistic action of choline deficiency with the basic toxicity of ethionine toward the acinar cells of the pancreas. The experimental model simulates closely the acute hemorrhagic pancreatitis with fat necrosis occurring in humans and may prove useful for exploring the pathogenesis of this condition.", "contents": "Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline-deficient diet. Female, albino mice were fed a choline-deficient diet containing 0.5% DL-ethionine. All animals died within 5 days due to the development of an acute hemorrhagic pancreatis with fat necrosis throughout the peritoneal cavity. The apancreatitis was characterized by a massive necrosis of the exocrine parenchyma with intense hemorrhage and inflammatory reaction of the stroma. The sequence of histologic and ultrastructural alterations occurring in the acinar cells of the pancreas were studied in mice fed the diet for 1, 2, and 3 days. Major findings consited of accumulation of zymogen granules, vacuolation due to foci of cytoplasmic degradation, and alterations in the morphology of the zymogen granules. The pancreatitis appears to be due to intraparenchymal activation of zymogens, resulting from a synergistic action of choline deficiency with the basic toxicity of ethionine toward the acinar cells of the pancreas. The experimental model simulates closely the acute hemorrhagic pancreatitis with fat necrosis occurring in humans and may prove useful for exploring the pathogenesis of this condition."}
{"id": "PMID:1094838", "title": "Vascularization of normal and neoplastic tissues grafted to the chick chorioallantois. Role of host and preexisting graft blood vessels.", "content": "Adult, embryonic, and tumor tissues were grafted to the chorioallantoic membrane of the chick embryo to determine whether blood vessels originally within implants were reused in the establishment of a new blood supply. Grafts were examined daily by in vivo stereomicroscopy and in histologic sections. Colloidal carbon injections into the host vasculature served to confirm the precise onset of graft circulation; Preexisting tumor blood vessels disintegrated by 24 hours after implantation and revascularization occurred at 3 days by penetration of proliferating host vessels into the tumor tissue. Adult tissues did not revascularize, and the original graft vasculature progressively disintegrated during the 9 days of observation, Most embryonic tissues revascularized in 1 or 2 days by reperfusion of the existing graft vasculature. Anastomosis of host and graft blood vessels seemed to result from connections between newly formed vascular sprouts arising from both vasculatures. This study indicates that only tumor grafts acquire their blood supply solely by formation of new blood vessels from the host microvasculature. By contrast revascularization of normal tissues, when it does occur, is predominately the result of perfusion of the preexisting graft blood vessels.", "contents": "Vascularization of normal and neoplastic tissues grafted to the chick chorioallantois. Role of host and preexisting graft blood vessels. Adult, embryonic, and tumor tissues were grafted to the chorioallantoic membrane of the chick embryo to determine whether blood vessels originally within implants were reused in the establishment of a new blood supply. Grafts were examined daily by in vivo stereomicroscopy and in histologic sections. Colloidal carbon injections into the host vasculature served to confirm the precise onset of graft circulation; Preexisting tumor blood vessels disintegrated by 24 hours after implantation and revascularization occurred at 3 days by penetration of proliferating host vessels into the tumor tissue. Adult tissues did not revascularize, and the original graft vasculature progressively disintegrated during the 9 days of observation, Most embryonic tissues revascularized in 1 or 2 days by reperfusion of the existing graft vasculature. Anastomosis of host and graft blood vessels seemed to result from connections between newly formed vascular sprouts arising from both vasculatures. This study indicates that only tumor grafts acquire their blood supply solely by formation of new blood vessels from the host microvasculature. By contrast revascularization of normal tissues, when it does occur, is predominately the result of perfusion of the preexisting graft blood vessels."}
{"id": "PMID:1094841", "title": "Depression, Delusions, and Drug Response.", "content": "Depressed patients with delusions were found to be markedly unresponsive to tricyclic drug therapy during an ongoing study of depressed patients. After four weeks of administration of imipramine hydrochloride, only 3 of 13 delusional depressed patients had responded to the drug, but 14 of 21 nondelusional depressed patients had responded. The authors conclude on the basis of these data and those of other researchers that delusional depressed patients should not be treated with tricyclic antidepressants and that current research with depressed patients should be reevaluated in the light of this finding.", "contents": "Depression, Delusions, and Drug Response. Depressed patients with delusions were found to be markedly unresponsive to tricyclic drug therapy during an ongoing study of depressed patients. After four weeks of administration of imipramine hydrochloride, only 3 of 13 delusional depressed patients had responded to the drug, but 14 of 21 nondelusional depressed patients had responded. The authors conclude on the basis of these data and those of other researchers that delusional depressed patients should not be treated with tricyclic antidepressants and that current research with depressed patients should be reevaluated in the light of this finding."}
{"id": "PMID:1094842", "title": "Methylphenidate and caffeine in the treatment of children with minimal brain dysfunction.", "content": "In this double-blind, crossover study of 8 boys with minimal brain dysfunction, 20 mg of methylphenidate daily was significantly better than 160 mg of caffeine in controlling behavior, especially impulsivity and hyperactivity, and methylphenidate alone was superior to decaffeinated coffee and placebo. The tentative results of this study confirm the primary position of sympathomimetics in the pharmacotherapy of minimal brain dysfunction and, in addition, suggest the involvement of dopamine pathways in this disorder. The authors urge further study of this hypothesis.", "contents": "Methylphenidate and caffeine in the treatment of children with minimal brain dysfunction. In this double-blind, crossover study of 8 boys with minimal brain dysfunction, 20 mg of methylphenidate daily was significantly better than 160 mg of caffeine in controlling behavior, especially impulsivity and hyperactivity, and methylphenidate alone was superior to decaffeinated coffee and placebo. The tentative results of this study confirm the primary position of sympathomimetics in the pharmacotherapy of minimal brain dysfunction and, in addition, suggest the involvement of dopamine pathways in this disorder. The authors urge further study of this hypothesis."}
{"id": "PMID:1094863", "title": "The nature and significance of sheep red blood cell rosettes.", "content": "Rosette formation between human thymus-derived (T) lymphocytes and sheep red blood cells (SRBC) was inhibited by T cell specific antisera but not by sera directed against other lymphocyte antigens. We conclude that the SRBC receptor is closely linked to T specific antigens.", "contents": "The nature and significance of sheep red blood cell rosettes. Rosette formation between human thymus-derived (T) lymphocytes and sheep red blood cells (SRBC) was inhibited by T cell specific antisera but not by sera directed against other lymphocyte antigens. We conclude that the SRBC receptor is closely linked to T specific antigens."}
{"id": "PMID:1094864", "title": "Evaluation of soluble-antigen fluorescent antibody test for antibodies to Trichinella spiralis in Experimentallly infected swine.", "content": "The soluble-antigen fluorescent antibody (SAFA) test was evaluated at intervals from 7 days to 1 year in 42 swine infected with 25, 100, 500, 2,500, 12,000, or 250,000 Trichinella spiralis larvae. Serums were test positive as early as 14 days after exposure in swine fed 25 larvae and as early as 7 days in swine fed 100 or 500 larvae. Serums of all swine fed larger numbers of larvae were test positive by day 17 and remained test positive for the duration of the experiment. Fluorescence obtained in tests of serums from noninfected, laboratory-raised swine varied significantly; field tests were not done. The test couldbe done with whole heparinized blood, dried blood, or serum eluted from paper disks. Time required for the test procedure was reduced to approximately 45 minutes. The SAFA test detected antibodies to T spiralis earlier in swine fed smaller numbers of larvae and for longer duration than any other serotest which has been evaluated in swine. The test, particularly if automated, seems adequate as a mass screening test for surveillance and control of trichinosis in swine.", "contents": "Evaluation of soluble-antigen fluorescent antibody test for antibodies to Trichinella spiralis in Experimentallly infected swine. The soluble-antigen fluorescent antibody (SAFA) test was evaluated at intervals from 7 days to 1 year in 42 swine infected with 25, 100, 500, 2,500, 12,000, or 250,000 Trichinella spiralis larvae. Serums were test positive as early as 14 days after exposure in swine fed 25 larvae and as early as 7 days in swine fed 100 or 500 larvae. Serums of all swine fed larger numbers of larvae were test positive by day 17 and remained test positive for the duration of the experiment. Fluorescence obtained in tests of serums from noninfected, laboratory-raised swine varied significantly; field tests were not done. The test couldbe done with whole heparinized blood, dried blood, or serum eluted from paper disks. Time required for the test procedure was reduced to approximately 45 minutes. The SAFA test detected antibodies to T spiralis earlier in swine fed smaller numbers of larvae and for longer duration than any other serotest which has been evaluated in swine. The test, particularly if automated, seems adequate as a mass screening test for surveillance and control of trichinosis in swine."}
{"id": "PMID:1094865", "title": "Macrophages in bovine milk.", "content": "The high vacuolated mononuclear cells in milk from normal bovine mammary glands, usually designated epithelial cells, were examined for properties characteristic of macrophages. In vitro, these cells concentrated neutral red into cytoplasmic vacuoles, adhered rapidly to and spread on glass, and phagocytosed viable Streptococcus agalactiae and Staphylococcus aureus. Neutral red uptake was demonstrated in vivo, and the vacuolated mononuclear cells phagocytosed formalinkilled S aureus in vivo. These results suggest that these cells are macrophages.", "contents": "Macrophages in bovine milk. The high vacuolated mononuclear cells in milk from normal bovine mammary glands, usually designated epithelial cells, were examined for properties characteristic of macrophages. In vitro, these cells concentrated neutral red into cytoplasmic vacuoles, adhered rapidly to and spread on glass, and phagocytosed viable Streptococcus agalactiae and Staphylococcus aureus. Neutral red uptake was demonstrated in vivo, and the vacuolated mononuclear cells phagocytosed formalinkilled S aureus in vivo. These results suggest that these cells are macrophages."}
{"id": "PMID:1094866", "title": "In utero immunization of calves against colisepticemia.", "content": "A total of 21 bovine fetuses was inoculated in utero with Escherichia coli antigen to determine if nonserotype-specific resistance to colisepticemia could be induced. (Seven of these fetuses were inoculated through the intact flank of the dam.) After birth, the calves were deprived of colostrum and challenge exposed to a serologically distinct E coli which killed nonvaccinated controls. Of 21 calves vaccinated as fetuses, 10 survived challenge exposure, 8 died of colisepticemia, and 3 were stillborn. Premature birth precluded an adequate period of vaccination in 6 of the calves that died of colisepticemia. A relationship was not observed between E coli serum antibody and survival after challenge exposure. The results indicate that in utero vaccination with a single serotype of E coli can result in heterogenetic protection against neonatal colisepticemia. However, the occurrence of stillbirth and premature birth in calves vaccinated in utero indicates  need for furthur research before field application of this technique.", "contents": "In utero immunization of calves against colisepticemia. A total of 21 bovine fetuses was inoculated in utero with Escherichia coli antigen to determine if nonserotype-specific resistance to colisepticemia could be induced. (Seven of these fetuses were inoculated through the intact flank of the dam.) After birth, the calves were deprived of colostrum and challenge exposed to a serologically distinct E coli which killed nonvaccinated controls. Of 21 calves vaccinated as fetuses, 10 survived challenge exposure, 8 died of colisepticemia, and 3 were stillborn. Premature birth precluded an adequate period of vaccination in 6 of the calves that died of colisepticemia. A relationship was not observed between E coli serum antibody and survival after challenge exposure. The results indicate that in utero vaccination with a single serotype of E coli can result in heterogenetic protection against neonatal colisepticemia. However, the occurrence of stillbirth and premature birth in calves vaccinated in utero indicates  need for furthur research before field application of this technique."}
{"id": "PMID:1094867", "title": "Propagation of Anaplasma marginale  in bovine lymph node cell culture.", "content": "Anaplasma marginale was propagated in cell cultures derived from bovine lymph node (LN). Treatment of host cells with diethylaminoethyl dextran (DEAE-D) before inoculation and centrifugation of inoculum onto the monolayers resulted in significant numerical increases of A marginale. The direct fluorescent antibody technique (FAT) was used for detection of the organism in culture. The rat was combined with the standard microscopic count procedure to obtain numerical estimates of the organism in cell culture. Infection of LN cells was irregular, with some cells containing many organisms and others containing none. The organisms were dispersed or in inclusions in the cytoplasm of LN cells. Numerical increases of organisms occurred within 6 hours and these were greatest at 12 to 24 hours. After 24 hours, the organisms decreased rapidly, but small numbers of them were observed for at least 7 days. The average generation time in culture was approximately 17.1 hours.", "contents": "Propagation of Anaplasma marginale  in bovine lymph node cell culture. Anaplasma marginale was propagated in cell cultures derived from bovine lymph node (LN). Treatment of host cells with diethylaminoethyl dextran (DEAE-D) before inoculation and centrifugation of inoculum onto the monolayers resulted in significant numerical increases of A marginale. The direct fluorescent antibody technique (FAT) was used for detection of the organism in culture. The rat was combined with the standard microscopic count procedure to obtain numerical estimates of the organism in cell culture. Infection of LN cells was irregular, with some cells containing many organisms and others containing none. The organisms were dispersed or in inclusions in the cytoplasm of LN cells. Numerical increases of organisms occurred within 6 hours and these were greatest at 12 to 24 hours. After 24 hours, the organisms decreased rapidly, but small numbers of them were observed for at least 7 days. The average generation time in culture was approximately 17.1 hours."}
{"id": "PMID:1094871", "title": "Frequency of isoproterenol hydrochloride-induced cardiac arrhythmia in 19 patients with chronic obstructive pulmonary disease: a prospective study.", "content": "The incidence of adrenergic-induced arrhythmias was studied in 19 patients with chronic obstructive lung disease uncomplicated by respiratory failure or cardiac desease. The effects of isoproterenol hydrochloride and a placebo were studied, using a randomized double blind technique and continuous monitoring of the cardiac rhythm. Two of the patients had episodes of arrhythmia associated only with nebulization of isoproterenol hydrochloride. Ten patients had no abnormality associated with isoproterenol hydrochloride or placebo nebulizations. In the remainder, episodes of arrhythmia occurred but could not be consistently correlated with placebo or drug nrbulizations. None of the patients was aware of any cardiac irregularity. A chi square analysis of the dataindicated no relationship between the isoproterenol nebulizations and cardiac arrhythmias. In our patients with uncomplicated chronic obstructive lung disease, nebulization of adrenergic bronchodilators seemed an infrequent cause of cardiac arrhythmias.", "contents": "Frequency of isoproterenol hydrochloride-induced cardiac arrhythmia in 19 patients with chronic obstructive pulmonary disease: a prospective study. The incidence of adrenergic-induced arrhythmias was studied in 19 patients with chronic obstructive lung disease uncomplicated by respiratory failure or cardiac desease. The effects of isoproterenol hydrochloride and a placebo were studied, using a randomized double blind technique and continuous monitoring of the cardiac rhythm. Two of the patients had episodes of arrhythmia associated only with nebulization of isoproterenol hydrochloride. Ten patients had no abnormality associated with isoproterenol hydrochloride or placebo nebulizations. In the remainder, episodes of arrhythmia occurred but could not be consistently correlated with placebo or drug nrbulizations. None of the patients was aware of any cardiac irregularity. A chi square analysis of the dataindicated no relationship between the isoproterenol nebulizations and cardiac arrhythmias. In our patients with uncomplicated chronic obstructive lung disease, nebulization of adrenergic bronchodilators seemed an infrequent cause of cardiac arrhythmias."}
{"id": "PMID:1094872", "title": "Postnatal growth and development of the lung.", "content": "The lung is qualitatively different at birth, compared to adult life, in many species. In the neonatal period, the peripheral part of the acinus is formed by primary saccules, and it is from the walls of these that alveoli develop postnatally in rats, mice, and rabbits. Alveolar development starts in utero in man and probably in several other species, including sheep, cat, and, probably, mammals with relatively advanced physical development at birth. A sequence of events is well established in rats and mice in the postnatal period. A phase of expansion first occurs, during which lung growth lags behind the increase in body weight and lungs enlarge primarily by expansion, but new tissue is laid down in the saccular wall. This is succeeded by a phase of tissue proliferation during which the saccule is subdivided by numerous secondary crests that develop in the saccular wall and result in the formation of alveoli. During this stage, the rate of lung growth exceeds the rate of body growth and there is a rapid increase in alveolar surface area. Elastic tissue plays a key role in the development of alveoli, and elastic fibers lie in the free margins of the secondary crests and around the mouths of the alveoli. At approximately 2 weeks of age, the rate of cellular multiplication and formation of alveoli is markedly diminished and the lung grows mainly by cellular enlargement, dilatation, and modification of pre-existing structures...", "contents": "Postnatal growth and development of the lung. The lung is qualitatively different at birth, compared to adult life, in many species. In the neonatal period, the peripheral part of the acinus is formed by primary saccules, and it is from the walls of these that alveoli develop postnatally in rats, mice, and rabbits. Alveolar development starts in utero in man and probably in several other species, including sheep, cat, and, probably, mammals with relatively advanced physical development at birth. A sequence of events is well established in rats and mice in the postnatal period. A phase of expansion first occurs, during which lung growth lags behind the increase in body weight and lungs enlarge primarily by expansion, but new tissue is laid down in the saccular wall. This is succeeded by a phase of tissue proliferation during which the saccule is subdivided by numerous secondary crests that develop in the saccular wall and result in the formation of alveoli. During this stage, the rate of lung growth exceeds the rate of body growth and there is a rapid increase in alveolar surface area. Elastic tissue plays a key role in the development of alveoli, and elastic fibers lie in the free margins of the secondary crests and around the mouths of the alveoli. At approximately 2 weeks of age, the rate of cellular multiplication and formation of alveoli is markedly diminished and the lung grows mainly by cellular enlargement, dilatation, and modification of pre-existing structures..."}
{"id": "PMID:1094873", "title": "Antibody to herpes-like virus in sarcoidosis.", "content": "Anti-hepes-like virus antibody titers in samples of serum drawn from 70 consecutive patients at the time of diagnosis of sarcoidosis were compared with those found in control subjects without sarcoidosis or granulomatous disease, matched for age, sex, and race. Antibody was detected in all 70 sarcoid and control sera; in general, titers were higher for the sarcoid patient than for the matched control subject, but the difference in the geometric mean titer was only 0.5 of a twofold dilution step. Only 21 (30 per cent) of the sarcoid and 8 (11 per cent) of the control sera had titers greater than or equal to 1:600. The difference in the geometric mean titer between patient and control subject did not vary significantly with sex, race, or type of sarcoidosis. A second sample of serum was drawn from 45 of the 70 sarcoid patients after an interval varying from 18 to 60 months. Among these 45 patients the titers were virtually unchanged: 39 (87 per cent) had the same titer on the second occasion, 4 were higher by one dilution, and 2 were lower by one dilution. There was no correlation between the titer of the initial sample of serum and the progression, regression, or attainment of inactivity of the sarcoidosis. From this study we conclude that the elevated anti-herpes-like virus antibody titer found in patients with sarcoidosis are most likely to be related to the ability of these patients to show enhanced humoral antibody responses.", "contents": "Antibody to herpes-like virus in sarcoidosis. Anti-hepes-like virus antibody titers in samples of serum drawn from 70 consecutive patients at the time of diagnosis of sarcoidosis were compared with those found in control subjects without sarcoidosis or granulomatous disease, matched for age, sex, and race. Antibody was detected in all 70 sarcoid and control sera; in general, titers were higher for the sarcoid patient than for the matched control subject, but the difference in the geometric mean titer was only 0.5 of a twofold dilution step. Only 21 (30 per cent) of the sarcoid and 8 (11 per cent) of the control sera had titers greater than or equal to 1:600. The difference in the geometric mean titer between patient and control subject did not vary significantly with sex, race, or type of sarcoidosis. A second sample of serum was drawn from 45 of the 70 sarcoid patients after an interval varying from 18 to 60 months. Among these 45 patients the titers were virtually unchanged: 39 (87 per cent) had the same titer on the second occasion, 4 were higher by one dilution, and 2 were lower by one dilution. There was no correlation between the titer of the initial sample of serum and the progression, regression, or attainment of inactivity of the sarcoidosis. From this study we conclude that the elevated anti-herpes-like virus antibody titer found in patients with sarcoidosis are most likely to be related to the ability of these patients to show enhanced humoral antibody responses."}
{"id": "PMID:1094874", "title": "Subdural hematoma in regularly hemodialyzed patients.", "content": "Thirteen of 394 (3.3%) regularly dialyzed patients of the Regional Kidney Disease Program developed subdural hematoma. The following factors contributed to formation of subdural hematoma: head trauma, ultrafiltration to control excessive accumulation of fluid and hypertension, anticoagulants, and frequent vascular access infection and clotting. Neurologic symptoms and signs, which may be similar to dialysis disequilibrium, aid only in signifying the presence, not the ultimate localization, of subdural hematoma. Our experience underscores the frequency of bilateral disease, irrespective of neurologic findings. Skull films, lumbar puncture, and electroencephalography were of little diagnostic help. Although valuable and safe, brain scanning was not as useful as desired due to occurrences of false-negative studies and failure to identify bilaterality of lesions. Cerebral angiography was always diagnostic. Surgical intervention yielded disappointing results, and only 2 patients (15%) survived. A review of 9 other patient reports is included.", "contents": "Subdural hematoma in regularly hemodialyzed patients. Thirteen of 394 (3.3%) regularly dialyzed patients of the Regional Kidney Disease Program developed subdural hematoma. The following factors contributed to formation of subdural hematoma: head trauma, ultrafiltration to control excessive accumulation of fluid and hypertension, anticoagulants, and frequent vascular access infection and clotting. Neurologic symptoms and signs, which may be similar to dialysis disequilibrium, aid only in signifying the presence, not the ultimate localization, of subdural hematoma. Our experience underscores the frequency of bilateral disease, irrespective of neurologic findings. Skull films, lumbar puncture, and electroencephalography were of little diagnostic help. Although valuable and safe, brain scanning was not as useful as desired due to occurrences of false-negative studies and failure to identify bilaterality of lesions. Cerebral angiography was always diagnostic. Surgical intervention yielded disappointing results, and only 2 patients (15%) survived. A review of 9 other patient reports is included."}
{"id": "PMID:1094875", "title": "D-penicillamine induced Goodpasture's syndrome in Wilson's disease.", "content": "Fatal pulmonary hemorrhages and rapidly progressive glomerulonephritis occurred in three patients with Wilson's disease (hepatolenticular degeneration) who had been treated with penicillamine for 2 to 31/2 years. Light microscopic studies of the kidneys showed severe glomerulonephritis with crescent formation, and the lungs showed intraalveolar hemorrhages. Although the clinical and pathologic abnormalities were those of Goodpasture's syndrome, immunofluorescence microscopic studies in the one case tested showed an interrupted, rather than linear, fluorescence pattern.", "contents": "D-penicillamine induced Goodpasture's syndrome in Wilson's disease. Fatal pulmonary hemorrhages and rapidly progressive glomerulonephritis occurred in three patients with Wilson's disease (hepatolenticular degeneration) who had been treated with penicillamine for 2 to 31/2 years. Light microscopic studies of the kidneys showed severe glomerulonephritis with crescent formation, and the lungs showed intraalveolar hemorrhages. Although the clinical and pathologic abnormalities were those of Goodpasture's syndrome, immunofluorescence microscopic studies in the one case tested showed an interrupted, rather than linear, fluorescence pattern."}
{"id": "PMID:1094879", "title": "Pulmonary function: relation to aging, cigarette habit, and mortality.", "content": "The relation of pulmonary function to aging and cigarette habits has been examined cross sectionally and longitudinally in the Framingham cohort. On cross-sectional analysis, women were found to have lower forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV-1) values than men even after adjusting for height. Their FEV-1 percent was, on the other hand, higher than those of men. As the population aged over 10 years, their FVC and FEV-1 declined 9% to 27% depending upon age and sex. The FEV-1 percent, however, showed a decline only in the older age groups. In a cross-sectional analysis, cigarette smoking showed an inverse association to FVC and FEV-1 percent. Longitudinally, cigarette smokers showed a more rapid decline in FVC in 10 years than nonsmokers. On giving up smoking their FVC became more like that of the nonsmokers. A striking relation of FVC to mortality was noted in both sexes, which is not accounted for by associated cigarette habits.", "contents": "Pulmonary function: relation to aging, cigarette habit, and mortality. The relation of pulmonary function to aging and cigarette habits has been examined cross sectionally and longitudinally in the Framingham cohort. On cross-sectional analysis, women were found to have lower forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV-1) values than men even after adjusting for height. Their FEV-1 percent was, on the other hand, higher than those of men. As the population aged over 10 years, their FVC and FEV-1 declined 9% to 27% depending upon age and sex. The FEV-1 percent, however, showed a decline only in the older age groups. In a cross-sectional analysis, cigarette smoking showed an inverse association to FVC and FEV-1 percent. Longitudinally, cigarette smokers showed a more rapid decline in FVC in 10 years than nonsmokers. On giving up smoking their FVC became more like that of the nonsmokers. A striking relation of FVC to mortality was noted in both sexes, which is not accounted for by associated cigarette habits."}
{"id": "PMID:1094880", "title": "Pneumocystis carinii pneumonia: a cluster of eleven cases.", "content": "An unusual cluster of 11 patients with Pneumocystis carinii pneumonia occurred in a 3-month period at Memorial Hospital, New York. Ten of the 11 patients had lymphoma or leukemia. In 7 patients, corticosteroid therapy was decreased or stopped shortly before pneumocystis pneumonia was diagnosed. The pediatric patients had extensive contact in the outpatient department, and three of them had roomed together, suggesting the possibility of man to man transmission. Tree of 4 adult patients with Hodgkin's disease and pneumocystis pneumonia had the same physician. This physician had an indirect immunofluorescent titer of 1:16, as did an infectious disease resident involved in the care of 6 patients. Three of 9 patients tested showed indirect immunofluorescent titers of 1:16 or above, with rising or falling titers on serial specimens. Although definitive evidence of communicability was not established, patients with suspected or proved pneumocystis pneumonia are now isolated in this hospital.", "contents": "Pneumocystis carinii pneumonia: a cluster of eleven cases. An unusual cluster of 11 patients with Pneumocystis carinii pneumonia occurred in a 3-month period at Memorial Hospital, New York. Ten of the 11 patients had lymphoma or leukemia. In 7 patients, corticosteroid therapy was decreased or stopped shortly before pneumocystis pneumonia was diagnosed. The pediatric patients had extensive contact in the outpatient department, and three of them had roomed together, suggesting the possibility of man to man transmission. Tree of 4 adult patients with Hodgkin's disease and pneumocystis pneumonia had the same physician. This physician had an indirect immunofluorescent titer of 1:16, as did an infectious disease resident involved in the care of 6 patients. Three of 9 patients tested showed indirect immunofluorescent titers of 1:16 or above, with rising or falling titers on serial specimens. Although definitive evidence of communicability was not established, patients with suspected or proved pneumocystis pneumonia are now isolated in this hospital."}
{"id": "PMID:1094889", "title": "The nature of \"spots\" on soft lenses.", "content": "Of 278 consecutive therapeutic soft lens wearers, 19 (6.8%) developed discrete lens opacities on the anterior (air) surface of the lens. The incidence rose to 16.6% in patients fitted for dry eye syndromes, to 19% in bullous keratopathy and to 20% in exposure keratitis and trichiasis, if worn for longer than 2 weeks. These opacities were not associated with medications. Scanning electron microscopy demonstrated cracks on the anterior lens surface associated with these opacities. Transmission electron microscopy demonstrated the opacities to be amorphous material with extensions of the contact lens polymer into the substance of these opacities. Lipid, as demonstrated by histochemical methods, was found in these opacities. It is likely that the combination of dryness, stress on the lens surface, and lipid deposition act to alter the structure of the plastic and cause these opacities.", "contents": "The nature of \"spots\" on soft lenses. Of 278 consecutive therapeutic soft lens wearers, 19 (6.8%) developed discrete lens opacities on the anterior (air) surface of the lens. The incidence rose to 16.6% in patients fitted for dry eye syndromes, to 19% in bullous keratopathy and to 20% in exposure keratitis and trichiasis, if worn for longer than 2 weeks. These opacities were not associated with medications. Scanning electron microscopy demonstrated cracks on the anterior lens surface associated with these opacities. Transmission electron microscopy demonstrated the opacities to be amorphous material with extensions of the contact lens polymer into the substance of these opacities. Lipid, as demonstrated by histochemical methods, was found in these opacities. It is likely that the combination of dryness, stress on the lens surface, and lipid deposition act to alter the structure of the plastic and cause these opacities."}
{"id": "PMID:1094893", "title": "Comparison of suturing techniques in keratoplasty for keratoconus.", "content": "The results of 48 consecutive penetrating keratoplasties for keratoconus were analyzed. The overall success rate was 93% clear grafts and there was no significant difference in the percentage of success, the amount of the spherical equivalent of the myopia, or the types of complications between the technique using 9-0 interrupted virgin silk sutures and 10-0 monofilament nylon running suture. The average astigmatic error was 25% less in the series employing the running suture.", "contents": "Comparison of suturing techniques in keratoplasty for keratoconus. The results of 48 consecutive penetrating keratoplasties for keratoconus were analyzed. The overall success rate was 93% clear grafts and there was no significant difference in the percentage of success, the amount of the spherical equivalent of the myopia, or the types of complications between the technique using 9-0 interrupted virgin silk sutures and 10-0 monofilament nylon running suture. The average astigmatic error was 25% less in the series employing the running suture."}
{"id": "PMID:1094895", "title": "Corneal intrastromal cyst following lamellar keratoplasty.", "content": "A case is reported documenting formation of an intrastromal corneal cyst following lamellar keratoplasty. A patient with keratoglobus sustained trauma to the left eye. The trauma resulted in rupture of the cornea, which was repaired with an overlay conreal graft. Two and one-half years later, an intrastromal fluid-filled cyst of the cornea was found in the visual axis. Cytologic examination of the aspirated cyst fluid revealed epithelial cells.", "contents": "Corneal intrastromal cyst following lamellar keratoplasty. A case is reported documenting formation of an intrastromal corneal cyst following lamellar keratoplasty. A patient with keratoglobus sustained trauma to the left eye. The trauma resulted in rupture of the cornea, which was repaired with an overlay conreal graft. Two and one-half years later, an intrastromal fluid-filled cyst of the cornea was found in the visual axis. Cytologic examination of the aspirated cyst fluid revealed epithelial cells."}
{"id": "PMID:1094896", "title": "Prevention of corneal opacification in open sky vitrectomy: Part I. An in vitro study.", "content": "We tested the effects of various physiologic solutions with known osmotic pressures on maintenance of corneal transparency in vitro and compared them with the effects of other solutions that exert both oncotic and osmotic pressures. Normal saline solution, 5% glucose and normal saline solution, tissue culture 199, and plasma were bathing solutions tested. Increases in hydration and endothelial cell damage for the various media were compared. We found that plasma preserved corneal dehydration and endothelial viability better than other physiologic solutions.", "contents": "Prevention of corneal opacification in open sky vitrectomy: Part I. An in vitro study. We tested the effects of various physiologic solutions with known osmotic pressures on maintenance of corneal transparency in vitro and compared them with the effects of other solutions that exert both oncotic and osmotic pressures. Normal saline solution, 5% glucose and normal saline solution, tissue culture 199, and plasma were bathing solutions tested. Increases in hydration and endothelial cell damage for the various media were compared. We found that plasma preserved corneal dehydration and endothelial viability better than other physiologic solutions."}
{"id": "PMID:1094904", "title": "Review of calcium methodologies.", "content": "A review of calcium methodologies for serum has been described. The analytical systems developed over the past century have been classified as to type beginning with gravimetry and extending to isotope dilution-mass spectrometry by covering all of the commonly used technics which have evolved during that period. Screening and referee procedures are discussed along with comparative sensitivities encountered between atomic absorption spectrophotometry and molecular absorption spectrophotometry. A procedure involving a simple direct reaction for serum calcium using cresolphthalein complexone is recommended in which high blanks are minimized by repressing the ionization of the color reagent on lowering the dielectric constant characteristics of the mixture with dimethylsulfoxide. Reaction characteristics, errors which can be encountered, normal ranges and an interpretative resume are included in its discussion.", "contents": "Review of calcium methodologies. A review of calcium methodologies for serum has been described. The analytical systems developed over the past century have been classified as to type beginning with gravimetry and extending to isotope dilution-mass spectrometry by covering all of the commonly used technics which have evolved during that period. Screening and referee procedures are discussed along with comparative sensitivities encountered between atomic absorption spectrophotometry and molecular absorption spectrophotometry. A procedure involving a simple direct reaction for serum calcium using cresolphthalein complexone is recommended in which high blanks are minimized by repressing the ionization of the color reagent on lowering the dielectric constant characteristics of the mixture with dimethylsulfoxide. Reaction characteristics, errors which can be encountered, normal ranges and an interpretative resume are included in its discussion."}
{"id": "PMID:1094936", "title": "[Physicochemical properties of some antibiotics].", "content": "Some physico-chemical properties of 3 antibiotics, i.e. kanamycin, neomycin and monomycin dried in the evaporating-drying apparatus IDA-ITE-6 constructed at the Institute of Technical Thermophysics of the UkSSR Academy of Sciences were studied. The properties of their 6 and 18 percent solutions and sorption properties of the antibiotic powders were described. It was found that the properties of the 3 antibiotics were close.", "contents": "[Physicochemical properties of some antibiotics]. Some physico-chemical properties of 3 antibiotics, i.e. kanamycin, neomycin and monomycin dried in the evaporating-drying apparatus IDA-ITE-6 constructed at the Institute of Technical Thermophysics of the UkSSR Academy of Sciences were studied. The properties of their 6 and 18 percent solutions and sorption properties of the antibiotic powders were described. It was found that the properties of the 3 antibiotics were close."}
{"id": "PMID:1094937", "title": "[Caffeine as an inhibitor of the conjugation transfer of R-factors. A study of certain aspects of the mechanism of action of caffeine on the conjugation transfer of R-factors].", "content": "Some aspects of the inhibitory effect of caffeine on conjugation transfer of R-factors described by the authors earlier were studied. The effect of the above substance on the donor and recipient competence was tested in experiments with cultivation of the parent strains for 18 hours in the presence of caffeine. For this purpose the effect of caffeine on reduction of the donor and recipient cell competence after starvation in a physiological solution was investigated. It was shown that caffeine markedly decreased the donor competence of strain 15-3Mdrd of E. coli in the experiments of both types. Caffeine also inhibited reduction of the recipient competence of strain C600 of E. coli after starvation without its changing on 18-hour treatment. For the study of the caffeine effect on formation of the conjugation pairs experiments were carried out with dilution of the conjugation mixture after definite intervals which practically stopped formation of new conjugation pairs and eliminated further effect of caffeine on conjugation. Under such conditions transfer of R-factors may occur in the conjugation pairs after elimination of caffeine by dilution, if they were formed in the presence of caffeine before the mixture dilution. The experiments showed that caffeine inhibited not the formation of the conjugation pairs but the genetic transfer of R-factors. In addition, it was found that the substance insignificantly inhibited the process of phenotypic manifestation of the resistance markers. Inhibition of conjugation R-transfer by caffeine was associated with its eliminating effect, since the concentrations used did not induce elimination of the resistance markers in R+ strains.", "contents": "[Caffeine as an inhibitor of the conjugation transfer of R-factors. A study of certain aspects of the mechanism of action of caffeine on the conjugation transfer of R-factors]. Some aspects of the inhibitory effect of caffeine on conjugation transfer of R-factors described by the authors earlier were studied. The effect of the above substance on the donor and recipient competence was tested in experiments with cultivation of the parent strains for 18 hours in the presence of caffeine. For this purpose the effect of caffeine on reduction of the donor and recipient cell competence after starvation in a physiological solution was investigated. It was shown that caffeine markedly decreased the donor competence of strain 15-3Mdrd of E. coli in the experiments of both types. Caffeine also inhibited reduction of the recipient competence of strain C600 of E. coli after starvation without its changing on 18-hour treatment. For the study of the caffeine effect on formation of the conjugation pairs experiments were carried out with dilution of the conjugation mixture after definite intervals which practically stopped formation of new conjugation pairs and eliminated further effect of caffeine on conjugation. Under such conditions transfer of R-factors may occur in the conjugation pairs after elimination of caffeine by dilution, if they were formed in the presence of caffeine before the mixture dilution. The experiments showed that caffeine inhibited not the formation of the conjugation pairs but the genetic transfer of R-factors. In addition, it was found that the substance insignificantly inhibited the process of phenotypic manifestation of the resistance markers. Inhibition of conjugation R-transfer by caffeine was associated with its eliminating effect, since the concentrations used did not induce elimination of the resistance markers in R+ strains."}
{"id": "PMID:1094938", "title": "[Level of \"internal\" resistance of E. coli cultures to benyzlpenicillin].", "content": "Interaction of benzylpenicillin inactivation by beta-lactamase and the growth of E. coli in the presence of various antibiotic concentrations was studied. It was found that penicillin inactivation began immediately after addition of the culture to the medium in spite of the fact that the number of the viable cells at that time decreased. The culture began to multiply only when benzylpenicillin concentration in the medium lowered up to a definite level (6-7 gamma/ml). Such a concentration must determine the level of the \"inner\" culture resistance to benzylpenicillin, not connected with the beta-lactamase effect. Moreover, the \"inner\" resistance proved to be the same for the cultures of E. coli, markedly differing by the amounts of beta-lactamase produced and the resistance levels to benzylpenicillin. Therefore, the routine method for determination of the \"inner\" resistance of the beta-lactamase-producing cultures to penicillins, using low innoculation doses produced higher results as compared to the real \"inner\" resistance. Significant differences observed between the values of the \"inner\" resistance (6-7 gamma/ml) and the MIC of benzylpenicillin at high innoculation doses with respect to E. coli 2 (129 gamma/ml) and E. coli 82 (15000 gamma/ml) may probably be explained by destruction of the drug by beta-lactamase.", "contents": "[Level of \"internal\" resistance of E. coli cultures to benyzlpenicillin]. Interaction of benzylpenicillin inactivation by beta-lactamase and the growth of E. coli in the presence of various antibiotic concentrations was studied. It was found that penicillin inactivation began immediately after addition of the culture to the medium in spite of the fact that the number of the viable cells at that time decreased. The culture began to multiply only when benzylpenicillin concentration in the medium lowered up to a definite level (6-7 gamma/ml). Such a concentration must determine the level of the \"inner\" culture resistance to benzylpenicillin, not connected with the beta-lactamase effect. Moreover, the \"inner\" resistance proved to be the same for the cultures of E. coli, markedly differing by the amounts of beta-lactamase produced and the resistance levels to benzylpenicillin. Therefore, the routine method for determination of the \"inner\" resistance of the beta-lactamase-producing cultures to penicillins, using low innoculation doses produced higher results as compared to the real \"inner\" resistance. Significant differences observed between the values of the \"inner\" resistance (6-7 gamma/ml) and the MIC of benzylpenicillin at high innoculation doses with respect to E. coli 2 (129 gamma/ml) and E. coli 82 (15000 gamma/ml) may probably be explained by destruction of the drug by beta-lactamase."}
{"id": "PMID:1094939", "title": "[Intrauterine sensitization to benzylpenicillin in an experiment on rabbits].", "content": "Antibodies of benzylpenicillin in the posterity of various ages of female rabbits were found with reaction of passive hemagglutination (RPHA) and degranulation of the basophiles after their immunization with the antibiotic. This provided an assumption of intrauterine sensitization. Treatment of the sera of the sensitized animals with 2-mercaptoethanol partially decreased their activity in the RPHA which indicated to the presence of the antibodies of classes IgG and IgM.", "contents": "[Intrauterine sensitization to benzylpenicillin in an experiment on rabbits]. Antibodies of benzylpenicillin in the posterity of various ages of female rabbits were found with reaction of passive hemagglutination (RPHA) and degranulation of the basophiles after their immunization with the antibiotic. This provided an assumption of intrauterine sensitization. Treatment of the sera of the sensitized animals with 2-mercaptoethanol partially decreased their activity in the RPHA which indicated to the presence of the antibodies of classes IgG and IgM."}
{"id": "PMID:1094940", "title": "[Theoretically probable assessment of the effectiveness of individualized combined antibiotic therapy of patients with suppurative inflammatory processes].", "content": "Efficiency of individual combined antibiotic therapy of patients was estimated in comparison to the use of single antibiotics of their fixed combinations without any special control group. Since the essence of the study was practically in successive appliance of the 2 methods of therapy to the same group of the patients, it was possible to carry out theoretically probable estimation of the final result as a summation effect of both methods and to define the probable result of the 2 nd method. The study of the results of the treatment of the patients with purulent-inflammatory processes showed significant advantages of the individual combined antibiotic therapy.", "contents": "[Theoretically probable assessment of the effectiveness of individualized combined antibiotic therapy of patients with suppurative inflammatory processes]. Efficiency of individual combined antibiotic therapy of patients was estimated in comparison to the use of single antibiotics of their fixed combinations without any special control group. Since the essence of the study was practically in successive appliance of the 2 methods of therapy to the same group of the patients, it was possible to carry out theoretically probable estimation of the final result as a summation effect of both methods and to define the probable result of the 2 nd method. The study of the results of the treatment of the patients with purulent-inflammatory processes showed significant advantages of the individual combined antibiotic therapy."}
{"id": "PMID:1094942", "title": "Effect of enrichment procedure upon auxotroph recovery in Escherichia coli K-12.", "content": "Proline-requiring auxotrophs are recovered preferentially after mutant enrichment procedures (e.g., penicillin) which perturb the cell envelope, but not after procedures (e.g., thymineless death) which affect other cellular targets. This probably stems from effects of penicillin and similar antibiotics upon proline metabolic and transport enzymes associated with the cell envelope.", "contents": "Effect of enrichment procedure upon auxotroph recovery in Escherichia coli K-12. Proline-requiring auxotrophs are recovered preferentially after mutant enrichment procedures (e.g., penicillin) which perturb the cell envelope, but not after procedures (e.g., thymineless death) which affect other cellular targets. This probably stems from effects of penicillin and similar antibiotics upon proline metabolic and transport enzymes associated with the cell envelope."}
{"id": "PMID:1094943", "title": "Selective inhibition of growth by the proline analogue thiazolidine-4-carboxylic acid and its probable mode of action in Saccharomyces cerevisiae.", "content": "Cells of Saccharomyces cerevisiae showed differential growth inhibition when cultured on various carbon sources in the presence of the proline analogue thiazolidine-4-carboxylic acid (TZ). On 0.5% yeast extract, 2% glucose and TZ (10 mg/ml) medium, growth lags from 8 to 10 h were observed, after which cells recovered and growth proceeded normally. Growth was totally inhibited on a medium of 0.5% yeast extract, 3% ethanol, and 5 mg of TZ per ml. This inhibition was not due to the inability of cells to undergo aerobic respiration, since similar media containing glycerol instead of ethanol allowed growth. Proline added to the culture medium reversed the lag on glucose and TZ medium but did not promote recovery on ethanol and TZ medium. TZ was found to have two probable modes of action in yeast. It was a noncompetitive inhibitor of yeast alcohol dehydrogenase, and it was also found to be incorporated into cellular protein. Uptake studies using (14)C-labeled TZ showed that the recovery on glucose was correlated with the progressive exclusion of the analogue from cells.", "contents": "Selective inhibition of growth by the proline analogue thiazolidine-4-carboxylic acid and its probable mode of action in Saccharomyces cerevisiae. Cells of Saccharomyces cerevisiae showed differential growth inhibition when cultured on various carbon sources in the presence of the proline analogue thiazolidine-4-carboxylic acid (TZ). On 0.5% yeast extract, 2% glucose and TZ (10 mg/ml) medium, growth lags from 8 to 10 h were observed, after which cells recovered and growth proceeded normally. Growth was totally inhibited on a medium of 0.5% yeast extract, 3% ethanol, and 5 mg of TZ per ml. This inhibition was not due to the inability of cells to undergo aerobic respiration, since similar media containing glycerol instead of ethanol allowed growth. Proline added to the culture medium reversed the lag on glucose and TZ medium but did not promote recovery on ethanol and TZ medium. TZ was found to have two probable modes of action in yeast. It was a noncompetitive inhibitor of yeast alcohol dehydrogenase, and it was also found to be incorporated into cellular protein. Uptake studies using (14)C-labeled TZ showed that the recovery on glucose was correlated with the progressive exclusion of the analogue from cells."}
{"id": "PMID:1094944", "title": "Effect of lincomycin and clindamycin on peptide chain initiation.", "content": "Lincomycin does not affect initiation factor-dependent formation of 70S initiation complexes formed with fmet-tRNA(F), the initiation triplet A-U-G, and 70S ribosomes, whereas its 7-chloro-derivative clindamycin substantially stimulates this process. Conversely, lincomycin stimulates nonenzymatic formation of the 70S complex, but clindamycin does not. Both antibiotics stimulate the assembly of non-enzymatically formed 70S initiation complexes with R(17) phage ribonucleic acid and exert little effect on those formed in the presence of initiation factors. The formation of 30S initiation complexes is stimulated or remains unaffected by lincomycin or clindamycin except when initiation occurs in the presence of very low Mg(2+) concentrations. In this case, both antibiotics inhibit the assembly of the 30S complexes regardless of the messenger present.", "contents": "Effect of lincomycin and clindamycin on peptide chain initiation. Lincomycin does not affect initiation factor-dependent formation of 70S initiation complexes formed with fmet-tRNA(F), the initiation triplet A-U-G, and 70S ribosomes, whereas its 7-chloro-derivative clindamycin substantially stimulates this process. Conversely, lincomycin stimulates nonenzymatic formation of the 70S complex, but clindamycin does not. Both antibiotics stimulate the assembly of non-enzymatically formed 70S initiation complexes with R(17) phage ribonucleic acid and exert little effect on those formed in the presence of initiation factors. The formation of 30S initiation complexes is stimulated or remains unaffected by lincomycin or clindamycin except when initiation occurs in the presence of very low Mg(2+) concentrations. In this case, both antibiotics inhibit the assembly of the 30S complexes regardless of the messenger present."}
{"id": "PMID:1094945", "title": "Comparative effect of tetracycline and doxycycline on the occurrence of resistant Escherichia coli in the fecal flora.", "content": "Antibiotic-induced changes in the fecal microflora after oral administration of tetracycline hydrochloride and doxycycline for 8 to 10 days were compared. A significant difference was noted in the concentrations of Escherichia coli resistant to tetracyclines. With tetracycline hydrochloride, there was a mean increase of approximately 10(4) resistant strains per g compared to only 10(1)/g for doxycycline. This difference is ascribed to reduced intestinal concentrations of bioactive drug with recommended oral dosage for doxycycline.", "contents": "Comparative effect of tetracycline and doxycycline on the occurrence of resistant Escherichia coli in the fecal flora. Antibiotic-induced changes in the fecal microflora after oral administration of tetracycline hydrochloride and doxycycline for 8 to 10 days were compared. A significant difference was noted in the concentrations of Escherichia coli resistant to tetracyclines. With tetracycline hydrochloride, there was a mean increase of approximately 10(4) resistant strains per g compared to only 10(1)/g for doxycycline. This difference is ascribed to reduced intestinal concentrations of bioactive drug with recommended oral dosage for doxycycline."}
{"id": "PMID:1094946", "title": "Relationship between polyene resistance and sterol compositions in Cryptococcus neoformans.", "content": "Six mutants of Cryptococcus neoformans resistant to nystatin and pimaricin and three mutants resistant to amphotericin B were isolated by ultraviolet irradiation techniques from two wild-type strains. The major sterols of the wild-type strains were Delta(7)-ergosten-3beta-ol and ergosterol. All six mutants resistant to nystatin and pimaricin showed either loss of ergosterol and concurrent production of Delta(7, 22)-ergostadien-3beta-ol and Delta(7)-ergosten-3beta-ol, or loss of both the wild-type sterols, with production of Delta(8(9))-ergosten-3beta-ol and Delta(5, 8(9), 22)-ergostatrien-3beta-ol. The mutants producing Delta(7, 22)-ergostadien-3beta-ol and Delta(7)-ergosten-3beta-ol showed relatively low levels of resistance to nystatin and pimaricin, whereas the mutants producing Delta(8(9))-ergosten-3beta-ol and Delta(5, 8(0), 22)-ergostatrien-3beta-ol showed a high level of resistance to either drug. Although highly resistant to amphotericin B, however, the three mutants produced sterol compositions identical to those of the wild types, indicating that the strains acquired resistance other than by alteration of the membrane sterols. The mutants producing Delta(8(9)) and Delta(5, 8(9), 22) sterols were not virulent for mice, showed reduced growth rates at 25 C, and failed to grow at 37 C. The other mutants showed a slightly reduced rate of growth both at 25 and 37 C, and the virulence in mice was slightly reduced in comparison with that of the wild types. These comparisons were on gross observations and were not statistically analyzed.", "contents": "Relationship between polyene resistance and sterol compositions in Cryptococcus neoformans. Six mutants of Cryptococcus neoformans resistant to nystatin and pimaricin and three mutants resistant to amphotericin B were isolated by ultraviolet irradiation techniques from two wild-type strains. The major sterols of the wild-type strains were Delta(7)-ergosten-3beta-ol and ergosterol. All six mutants resistant to nystatin and pimaricin showed either loss of ergosterol and concurrent production of Delta(7, 22)-ergostadien-3beta-ol and Delta(7)-ergosten-3beta-ol, or loss of both the wild-type sterols, with production of Delta(8(9))-ergosten-3beta-ol and Delta(5, 8(9), 22)-ergostatrien-3beta-ol. The mutants producing Delta(7, 22)-ergostadien-3beta-ol and Delta(7)-ergosten-3beta-ol showed relatively low levels of resistance to nystatin and pimaricin, whereas the mutants producing Delta(8(9))-ergosten-3beta-ol and Delta(5, 8(0), 22)-ergostatrien-3beta-ol showed a high level of resistance to either drug. Although highly resistant to amphotericin B, however, the three mutants produced sterol compositions identical to those of the wild types, indicating that the strains acquired resistance other than by alteration of the membrane sterols. The mutants producing Delta(8(9)) and Delta(5, 8(9), 22) sterols were not virulent for mice, showed reduced growth rates at 25 C, and failed to grow at 37 C. The other mutants showed a slightly reduced rate of growth both at 25 and 37 C, and the virulence in mice was slightly reduced in comparison with that of the wild types. These comparisons were on gross observations and were not statistically analyzed."}
{"id": "PMID:1094947", "title": "Nystatin-induced changes in Saccharomyces cerevisiae.", "content": "Nystatin (5 to 10 mug/ml) was fungicidal to Saccharomyces cerevisiae NCYC 361. There was an initial rapid uptake of nystatin by the yeast cells at 30 C, the uptake being pH dependent, with a maximum at low pH values (3 to 4). Photomicrographs indicated that nystatin-treated cells became granular. The effect of growth temperature on the subsequent sensitivity of the yeast cells to nystatin was investigated. Cells grown at 20 C were the most susceptible to all concentrations of nystatin tested; as the pretreatment growth temperature increased, the subsequent uptake of antibiotic by such cells decreased both in intensity and in overall extent, the most marked difference occurring between 20 and 25 C grown cells. Leakage of K(+) and the optical density of suspensions treated with nystatin were similarly affected by growth temperature. Differences in the electrophoretic mobilities of cells grown at different temperatures were also observed. The total ergosterol content of 20 C grown cells was markedly higher than that of 40 C grown cells, with 30 C grown cells having an intermediate value.", "contents": "Nystatin-induced changes in Saccharomyces cerevisiae. Nystatin (5 to 10 mug/ml) was fungicidal to Saccharomyces cerevisiae NCYC 361. There was an initial rapid uptake of nystatin by the yeast cells at 30 C, the uptake being pH dependent, with a maximum at low pH values (3 to 4). Photomicrographs indicated that nystatin-treated cells became granular. The effect of growth temperature on the subsequent sensitivity of the yeast cells to nystatin was investigated. Cells grown at 20 C were the most susceptible to all concentrations of nystatin tested; as the pretreatment growth temperature increased, the subsequent uptake of antibiotic by such cells decreased both in intensity and in overall extent, the most marked difference occurring between 20 and 25 C grown cells. Leakage of K(+) and the optical density of suspensions treated with nystatin were similarly affected by growth temperature. Differences in the electrophoretic mobilities of cells grown at different temperatures were also observed. The total ergosterol content of 20 C grown cells was markedly higher than that of 40 C grown cells, with 30 C grown cells having an intermediate value."}
{"id": "PMID:1094948", "title": "Bacteriological studies with morphine-like narcotics: relevance to narcotic actions in mammals?", "content": "A search for active bacterial growth inhibitors among seven highly potent morphine-like narcotics revealed that NIH 7591 and etorphine inhibited the rates of growth of Escherichia coli by 50% at 1.9 x 10(-4) M. Bacterial cultures escaped from growth inhibition by NIH 7591 after times which were proportional to the drug concentrations and inversely proportional to the initial bacterial densities. Populations of E. coli could adapt to resist and cross-resist growth inhibitions by NIH 7591 and phenazocine. Resistance was lost after growth in drug-free medium for a few doubling times. The agonist-antagonist pair, etorphine and diprenorphine, inhibited growth of E. coli additively without any indication of antagonism. Actions of narcotics in bacteria is considered a theme in its own right.", "contents": "Bacteriological studies with morphine-like narcotics: relevance to narcotic actions in mammals? A search for active bacterial growth inhibitors among seven highly potent morphine-like narcotics revealed that NIH 7591 and etorphine inhibited the rates of growth of Escherichia coli by 50% at 1.9 x 10(-4) M. Bacterial cultures escaped from growth inhibition by NIH 7591 after times which were proportional to the drug concentrations and inversely proportional to the initial bacterial densities. Populations of E. coli could adapt to resist and cross-resist growth inhibitions by NIH 7591 and phenazocine. Resistance was lost after growth in drug-free medium for a few doubling times. The agonist-antagonist pair, etorphine and diprenorphine, inhibited growth of E. coli additively without any indication of antagonism. Actions of narcotics in bacteria is considered a theme in its own right."}
{"id": "PMID:1094949", "title": "Cinoxacin: in vitro antibacterial studies of a new synthetic organic acid.", "content": "Cinoxacin (compound 64716) is a synthetic organic acid with antibacterial activity against most aerobic gram-negative bacilli. Minimal inhibitory concentrations of cinoxacin (agar-dilution method) were determined for 419 strains. Escherichia coli was the most susceptible group of organisms. The majority of Klebsiella sp., Enterobacter sp., Proteus sp., and Serratia marcescens were inhibited by 8 mug of cinoxacin per ml. Pseudomonas aeruginosa and all gram-positive isolates tested were resistant to 64 mug or less of cinoxacin per ml. Zones of inhibition using a 30-mug disk correlated well with agar-dilution minimal inhibitory concentrations (r = -0.9). Cinoxacin was bactericidal when tested with inocula of 5 x 10(6) organisms per ml. Resistance to cinoxacin was readily developed in all three strains tested by serial passage on drug-containing agar. The in vitro properties of this agent were similar to those of nalidixic acid.", "contents": "Cinoxacin: in vitro antibacterial studies of a new synthetic organic acid. Cinoxacin (compound 64716) is a synthetic organic acid with antibacterial activity against most aerobic gram-negative bacilli. Minimal inhibitory concentrations of cinoxacin (agar-dilution method) were determined for 419 strains. Escherichia coli was the most susceptible group of organisms. The majority of Klebsiella sp., Enterobacter sp., Proteus sp., and Serratia marcescens were inhibited by 8 mug of cinoxacin per ml. Pseudomonas aeruginosa and all gram-positive isolates tested were resistant to 64 mug or less of cinoxacin per ml. Zones of inhibition using a 30-mug disk correlated well with agar-dilution minimal inhibitory concentrations (r = -0.9). Cinoxacin was bactericidal when tested with inocula of 5 x 10(6) organisms per ml. Resistance to cinoxacin was readily developed in all three strains tested by serial passage on drug-containing agar. The in vitro properties of this agent were similar to those of nalidixic acid."}
{"id": "PMID:1094950", "title": "Comparative effects of amoxycillin and ampicillin in the treatment of experimental mouse infections.", "content": "Amoxycillin was significantly more active than ampicillin in the treatment of intraperitoneal mouse infections when administered by oral and parenteral routes, although the causal bacteria were equally susceptible in vitro to the two penicillins. Amoxycillin produced higher antibiotic blood concentrations in mice than ampicillin after oral administration, and this was a possible explanation for the superior oral activity of amoxycillin. In contrast, antibiotic blood concentrations were the same for both compounds after subcutaneous injection, but it was demonstrated that amoxycillin was more effective than ampicillin by this route in reducing bacterial counts in the peritoneal cavity and in the blood of mice infected with Escherichia coli. Amoxycillin was also significantly more active than ampicillin in the treatment of infection by intraperitoneal dosing as a result of greater bactericidal activity in infected mice together with the production of higher antibiotic blood levels. The results of these studies on the effects of parental treatment of experimental infections with the two penicillins show that the superior chemotherapeutic activity of amoxycillin was associated with the greater bactericidal activity of amoxycillin in vivo and with differences in the distribution of the two penicillins in the infected animal.", "contents": "Comparative effects of amoxycillin and ampicillin in the treatment of experimental mouse infections. Amoxycillin was significantly more active than ampicillin in the treatment of intraperitoneal mouse infections when administered by oral and parenteral routes, although the causal bacteria were equally susceptible in vitro to the two penicillins. Amoxycillin produced higher antibiotic blood concentrations in mice than ampicillin after oral administration, and this was a possible explanation for the superior oral activity of amoxycillin. In contrast, antibiotic blood concentrations were the same for both compounds after subcutaneous injection, but it was demonstrated that amoxycillin was more effective than ampicillin by this route in reducing bacterial counts in the peritoneal cavity and in the blood of mice infected with Escherichia coli. Amoxycillin was also significantly more active than ampicillin in the treatment of infection by intraperitoneal dosing as a result of greater bactericidal activity in infected mice together with the production of higher antibiotic blood levels. The results of these studies on the effects of parental treatment of experimental infections with the two penicillins show that the superior chemotherapeutic activity of amoxycillin was associated with the greater bactericidal activity of amoxycillin in vivo and with differences in the distribution of the two penicillins in the infected animal."}
{"id": "PMID:1094951", "title": "Antibiogram and lipid analysis of a pigmented strain of Serratia marcescens and its nonpigmented variants.", "content": "Antibiograms and lipid analyses of Serratia marcescens pigmented strain 08 and its nonpigmented variants are compared. The overall lack of significant differences between pigmented and nonpigmented strains suggests that the role of pigment formation may not be related to antibiotic susceptibility.", "contents": "Antibiogram and lipid analysis of a pigmented strain of Serratia marcescens and its nonpigmented variants. Antibiograms and lipid analyses of Serratia marcescens pigmented strain 08 and its nonpigmented variants are compared. The overall lack of significant differences between pigmented and nonpigmented strains suggests that the role of pigment formation may not be related to antibiotic susceptibility."}
{"id": "PMID:1094952", "title": "Streptomycin dependence in Escherichia coli: effects of antibiotic deprivation on ribosomes.", "content": "The inhibition of cell division and the ultimate loss of viability after removal of streptomycin from growing cultures of streptomycin-dependent bacteria are not the result of \"unbalanced growth\" or of the breakdown of ribosomes. The streptomycin-dependent strain of Escherichia coli K-12 studied continued to synthesize ribonucleic acid (RNA) and protein during streptomycin starvation. There was no evidence of a gross imbalance in the ratio of RNA to protein synthesized or of selective degradation of either protein or RNA. Using the sedimentation of subunits in sucrose as the criterion, normal ribosomes were synthesized even after 18 h of streptomycin deprivation, although the rates of appearance of mature 30S and 50S subunits decreased with time of deprivation. Once formed, these ribosomes appeared stable, as did those synthesized before the onset of starvation. Ribosomes isolated from starved dependent cells were as \"functional\" as ribosomes from cells grown with streptomycin in their capacity to bind aminoacyl-transfer RNA in response to polyuridylic acid or natural messenger RNA to interconvert between active and inactive transfer RNA binding states, and to synthesize proteins in cell-free systems. The effects are consistent with an impaired rate of synthesis of ribosomal components or assembly of ribosomes resulting in a continually diminishing rate of protein synthesis. The effect on cell division may be the result of a decreased rate of protein synthesis in general and the requirement for a specific protein(s) in particular.", "contents": "Streptomycin dependence in Escherichia coli: effects of antibiotic deprivation on ribosomes. The inhibition of cell division and the ultimate loss of viability after removal of streptomycin from growing cultures of streptomycin-dependent bacteria are not the result of \"unbalanced growth\" or of the breakdown of ribosomes. The streptomycin-dependent strain of Escherichia coli K-12 studied continued to synthesize ribonucleic acid (RNA) and protein during streptomycin starvation. There was no evidence of a gross imbalance in the ratio of RNA to protein synthesized or of selective degradation of either protein or RNA. Using the sedimentation of subunits in sucrose as the criterion, normal ribosomes were synthesized even after 18 h of streptomycin deprivation, although the rates of appearance of mature 30S and 50S subunits decreased with time of deprivation. Once formed, these ribosomes appeared stable, as did those synthesized before the onset of starvation. Ribosomes isolated from starved dependent cells were as \"functional\" as ribosomes from cells grown with streptomycin in their capacity to bind aminoacyl-transfer RNA in response to polyuridylic acid or natural messenger RNA to interconvert between active and inactive transfer RNA binding states, and to synthesize proteins in cell-free systems. The effects are consistent with an impaired rate of synthesis of ribosomal components or assembly of ribosomes resulting in a continually diminishing rate of protein synthesis. The effect on cell division may be the result of a decreased rate of protein synthesis in general and the requirement for a specific protein(s) in particular."}
{"id": "PMID:1094958", "title": "Immunologic studies in patients with sarcoidosis and cryptococcosis.", "content": "Immunologic studies were performed in two patients with sarcoidosis, who developed cryptococcosis. Polymorphonuclear leukocyte function, complement, and serum antibodies were normal. Both patients had depressed cell-mediated immunity (cmi) to Cryptococcus neoformans and other antigens that persisted after therapy for their infection. These findings suggest that the patients' impaired CMI predisposed them to cryptococcal infection, which complicated their sarcoidosis. Evaluation of sarcoidosis cases should include studies of immune function, and the possibility of a secondary infection should be considered in patients with long-standing sarcoidosis, who develop unexpected changes in their clinical status.", "contents": "Immunologic studies in patients with sarcoidosis and cryptococcosis. Immunologic studies were performed in two patients with sarcoidosis, who developed cryptococcosis. Polymorphonuclear leukocyte function, complement, and serum antibodies were normal. Both patients had depressed cell-mediated immunity (cmi) to Cryptococcus neoformans and other antigens that persisted after therapy for their infection. These findings suggest that the patients' impaired CMI predisposed them to cryptococcal infection, which complicated their sarcoidosis. Evaluation of sarcoidosis cases should include studies of immune function, and the possibility of a secondary infection should be considered in patients with long-standing sarcoidosis, who develop unexpected changes in their clinical status."}
{"id": "PMID:1094959", "title": "Microcytotoxicity and serum blocking factors in malignant melanoma and halo nevus.", "content": "Microcytotoxicity assays of patients with malignant melanoma and halo nevi were performed. No good correlation could be found between percent cell inhibition and histopathological level of melanoma or the clinical staging. The percent cell inhibition was usually an index of response to vaccinia virus immunotherapy. Actively regressing halo nevi showed high levels of percent cell inhibition, whereas inactive halo nevi had low levels of percent cell inhibition and blocking factor. Immunologic reactivity to melanoma cells may be a common feature of melanoma and halo nevus.", "contents": "Microcytotoxicity and serum blocking factors in malignant melanoma and halo nevus. Microcytotoxicity assays of patients with malignant melanoma and halo nevi were performed. No good correlation could be found between percent cell inhibition and histopathological level of melanoma or the clinical staging. The percent cell inhibition was usually an index of response to vaccinia virus immunotherapy. Actively regressing halo nevi showed high levels of percent cell inhibition, whereas inactive halo nevi had low levels of percent cell inhibition and blocking factor. Immunologic reactivity to melanoma cells may be a common feature of melanoma and halo nevus."}
{"id": "PMID:1094960", "title": "Double-blind comparison of halcinonide and betamethasone valerate. Use with occlusive dressings in psoriasis treatment.", "content": "In a double-blind, paired-comparison study of the effectiveness of corticosteroid creams used under occlusion for the treatment of moderate to severe psoriasis, a new drug, halcinonide cream, was superior to betamethasone valerate cream, a steroid widely and effectively used for the treatment of psoriasis. This superiority was demonstrated in patients after both one and two weeks of treatment.", "contents": "Double-blind comparison of halcinonide and betamethasone valerate. Use with occlusive dressings in psoriasis treatment. In a double-blind, paired-comparison study of the effectiveness of corticosteroid creams used under occlusion for the treatment of moderate to severe psoriasis, a new drug, halcinonide cream, was superior to betamethasone valerate cream, a steroid widely and effectively used for the treatment of psoriasis. This superiority was demonstrated in patients after both one and two weeks of treatment."}
{"id": "PMID:1094961", "title": "Cat-scratch disease skin test. Studies of specificity and histopathologic features.", "content": "Microscopical examination of biopsy specimens from cat-scratch skin test sites, performed in patients with cat-scratch disease (CSD), sarcoidosis, tuberculosis, and other granulomatous diseases, showed noncaseating granulomatous dermal inflammation in 11 of 12 patients with CSD and in some patients with sarcoidosis or tuberculosis. Biopsy of cat-scratch skin test sites may be a useful ancillary procedure in diagnosing CSD if other granulomatous diseases are excluded.", "contents": "Cat-scratch disease skin test. Studies of specificity and histopathologic features. Microscopical examination of biopsy specimens from cat-scratch skin test sites, performed in patients with cat-scratch disease (CSD), sarcoidosis, tuberculosis, and other granulomatous diseases, showed noncaseating granulomatous dermal inflammation in 11 of 12 patients with CSD and in some patients with sarcoidosis or tuberculosis. Biopsy of cat-scratch skin test sites may be a useful ancillary procedure in diagnosing CSD if other granulomatous diseases are excluded."}
{"id": "PMID:1094962", "title": "Percutaneous absorption of salicylic acid.", "content": "The potential hazards of repeated topical application of salicylic acid under occlusion to large areas of the body was evaluated by measuring the percutaneous absorption and serum salicylate concentrations in four patients with active psoriasis. Serum salicylate concentrations never exceeded 5 mg/100 ml in any of the patients, and although greater than 60 percent of the salicylic acid applied was absorbed, no evidence of accumulation or toxicity was observed. This form of treatment appears to present little potential hazard even in patients with extensive skin disease. Therapy could be hazardous for patients with impaired hepatic or renal function or for smaller children. The urinary excretory products of salicylate metabolism were compared following topical and intravenous salicylate administration to determine if the skin plays any part in the biotransformation of salicylate during percutaneous absorption. Our data are too limited and inconclusive to answer this question.", "contents": "Percutaneous absorption of salicylic acid. The potential hazards of repeated topical application of salicylic acid under occlusion to large areas of the body was evaluated by measuring the percutaneous absorption and serum salicylate concentrations in four patients with active psoriasis. Serum salicylate concentrations never exceeded 5 mg/100 ml in any of the patients, and although greater than 60 percent of the salicylic acid applied was absorbed, no evidence of accumulation or toxicity was observed. This form of treatment appears to present little potential hazard even in patients with extensive skin disease. Therapy could be hazardous for patients with impaired hepatic or renal function or for smaller children. The urinary excretory products of salicylate metabolism were compared following topical and intravenous salicylate administration to determine if the skin plays any part in the biotransformation of salicylate during percutaneous absorption. Our data are too limited and inconclusive to answer this question."}
{"id": "PMID:1094963", "title": "Identification of foreign material in lung by energy dispersive x-ray analysis. A new approach to silicosis.", "content": "Conventional methods for the detection of foreign material in the lungs are not only difficult but make it impossible to study any relationship  between the material and the surrounding tissue. The present study employs energy dispersive x-ray analysis and scanning electron microscopy as the basis of a rapid and accurate method for determining the amount of silicon in the lungs of normal subjects and subjects with silicosis. A statistical evaluation of the data suggests that a silicon/sulfur ratio below 0.2 may be considered normal, whereas, a ratio above 0.3 may be considered to indicate silicosis.", "contents": "Identification of foreign material in lung by energy dispersive x-ray analysis. A new approach to silicosis. Conventional methods for the detection of foreign material in the lungs are not only difficult but make it impossible to study any relationship  between the material and the surrounding tissue. The present study employs energy dispersive x-ray analysis and scanning electron microscopy as the basis of a rapid and accurate method for determining the amount of silicon in the lungs of normal subjects and subjects with silicosis. A statistical evaluation of the data suggests that a silicon/sulfur ratio below 0.2 may be considered normal, whereas, a ratio above 0.3 may be considered to indicate silicosis."}
{"id": "PMID:1094964", "title": "MIF production of lymphocytes from patients with rheumatoid arthritis with antigen-antibody complexes.", "content": "Immune complexes, human erythrocytes coated with human IgG antibody, inhibit the migration of lymphocytes from rheumatoid arthritis (RA) patients. No correlation could be observed between migration inhibition and Waaler-Rose titre (sheep cell agglutination titre) in inidvidual patients. Production of migration inhibitory factor (MIF) could be detected in 16 of 20 cultures of RA lymphocytes incubated with antigen-antibody complexes, when the supernates were tested with guinea pig macrophages. Only two supernates of healthy persons showed migration inhibition activity under these conditions. The results suggest that migration inhibition by antigen antibody complexes in RA represents a true reaction of delayed hypersensitivity.", "contents": "MIF production of lymphocytes from patients with rheumatoid arthritis with antigen-antibody complexes. Immune complexes, human erythrocytes coated with human IgG antibody, inhibit the migration of lymphocytes from rheumatoid arthritis (RA) patients. No correlation could be observed between migration inhibition and Waaler-Rose titre (sheep cell agglutination titre) in inidvidual patients. Production of migration inhibitory factor (MIF) could be detected in 16 of 20 cultures of RA lymphocytes incubated with antigen-antibody complexes, when the supernates were tested with guinea pig macrophages. Only two supernates of healthy persons showed migration inhibition activity under these conditions. The results suggest that migration inhibition by antigen antibody complexes in RA represents a true reaction of delayed hypersensitivity."}
{"id": "PMID:1094965", "title": "Feprazone, a new anti-inflammatory agent. Studies of potency and gastrointestinal tolerance.", "content": "Two studies are reported; a double-blind cross-over trial of feprazone 600 mg daily and aspirin 3.6 g daily in the treatment of rheumatoid arthritis, and an uncontrolled open study of gastrointestinal tolerance in twenty rheumatoid arthritis patients with known intolerance to other drugs. The first study showed that feprazone was significantly superior to aspirin in all the parameters tested. In the second study all twenty patients showed an improvement of their gastrointestinal symptoms, nineteen reporting no symptoms at all when taking the new preparation.", "contents": "Feprazone, a new anti-inflammatory agent. Studies of potency and gastrointestinal tolerance. Two studies are reported; a double-blind cross-over trial of feprazone 600 mg daily and aspirin 3.6 g daily in the treatment of rheumatoid arthritis, and an uncontrolled open study of gastrointestinal tolerance in twenty rheumatoid arthritis patients with known intolerance to other drugs. The first study showed that feprazone was significantly superior to aspirin in all the parameters tested. In the second study all twenty patients showed an improvement of their gastrointestinal symptoms, nineteen reporting no symptoms at all when taking the new preparation."}
{"id": "PMID:1094967", "title": "Closure of colostomy.", "content": "We analyzed the records of 77 cases of loop colostomy closure in Vietnam War Casualties. All records were complete from the date of injury to discharge following colostomy closure. Simple of the loop colostomy was performed in 44 patients and resection of the stoma and reanastomosis of bowel segments was performed in 33 patients. Average operating time for simple closure of the loop was 70 minutes compared to 115 minutes for resection and anastomosis. Nasogastric suction was used less frequently and for a shorter time with simple loop closure. The total postoperative complication rate was 9% with simple loop closure as compared to 24% for resection and anastomosis. Simple closure of the loop described in this report is technically easier and as safe as resection of the stoma and reanastomosis.", "contents": "Closure of colostomy. We analyzed the records of 77 cases of loop colostomy closure in Vietnam War Casualties. All records were complete from the date of injury to discharge following colostomy closure. Simple of the loop colostomy was performed in 44 patients and resection of the stoma and reanastomosis of bowel segments was performed in 33 patients. Average operating time for simple closure of the loop was 70 minutes compared to 115 minutes for resection and anastomosis. Nasogastric suction was used less frequently and for a shorter time with simple loop closure. The total postoperative complication rate was 9% with simple loop closure as compared to 24% for resection and anastomosis. Simple closure of the loop described in this report is technically easier and as safe as resection of the stoma and reanastomosis."}
{"id": "PMID:1094968", "title": "Lung thermal volume in pulmonary edema: effect of positive end expiratory pressure.", "content": "Effects of intermittent (IPPB) and positive eng-expiratory pressure (PEEP) ventilation on accumulation of pulmonary edema were compared, in dogs, after infusion of oleic acid. Pulmonary extravascular water was approximated as lung thermal volume (LTV), a double indicator method based on differential transit time for simultaneously injected right-to-left conductivity and thermal pulses. LTV was found to be decreased in animals treated with PEEP. The possibility that observed LTV changes reflect only the effect of PEEP on flow distribution, not lung water, was examined by alternating PEEP  and IPPB; short-term changes in LTV did not occur. Mean values of other factors influencing pulmonary water transfer, e.g., pulmonary capillary wedge pressure, serum protein, arterial blood gasses, were not significantly different with or without PEEP. It was concluded that, for the oleic acid lesion, PEEP effects a small reduction in the rate of accumulation of pulmonary edema.", "contents": "Lung thermal volume in pulmonary edema: effect of positive end expiratory pressure. Effects of intermittent (IPPB) and positive eng-expiratory pressure (PEEP) ventilation on accumulation of pulmonary edema were compared, in dogs, after infusion of oleic acid. Pulmonary extravascular water was approximated as lung thermal volume (LTV), a double indicator method based on differential transit time for simultaneously injected right-to-left conductivity and thermal pulses. LTV was found to be decreased in animals treated with PEEP. The possibility that observed LTV changes reflect only the effect of PEEP on flow distribution, not lung water, was examined by alternating PEEP  and IPPB; short-term changes in LTV did not occur. Mean values of other factors influencing pulmonary water transfer, e.g., pulmonary capillary wedge pressure, serum protein, arterial blood gasses, were not significantly different with or without PEEP. It was concluded that, for the oleic acid lesion, PEEP effects a small reduction in the rate of accumulation of pulmonary edema."}
{"id": "PMID:1094969", "title": "Quantitative bacterial analysis of comparative wound irrigations.", "content": "It is a biologic fact that all open wounds contain bacteria and remain contaminated with varying levels of bacteria until successful wound closure has been accomplished. The sine qua non in the management of the contaminated wound has been and remains adequate sharp debridement. In a standardized experimental model, three methods of irrigation were compared employing quantitative bacteriology of tissue to evaluate their effectiveness at decreasing bacterial levels and reducing wound infection. The pulsating jet lavate was found to be significantly better than gravity flow irrigation or wound irrigation with a bulb syringe. These data on tissue biopsies support previous reports using surface and wound exudate cultures.", "contents": "Quantitative bacterial analysis of comparative wound irrigations. It is a biologic fact that all open wounds contain bacteria and remain contaminated with varying levels of bacteria until successful wound closure has been accomplished. The sine qua non in the management of the contaminated wound has been and remains adequate sharp debridement. In a standardized experimental model, three methods of irrigation were compared employing quantitative bacteriology of tissue to evaluate their effectiveness at decreasing bacterial levels and reducing wound infection. The pulsating jet lavate was found to be significantly better than gravity flow irrigation or wound irrigation with a bulb syringe. These data on tissue biopsies support previous reports using surface and wound exudate cultures."}
{"id": "PMID:1094970", "title": "Choice of abdominal operative incision in the obese patient: a study using blood gas measurements.", "content": "Although many factors may influence the choice of the operative incision, physiologic safety in  terms of the degree of postoperative hypoxemia should be considered. No data currently exist to delineate in markedly obese patients the effect of the operative incision on the extent or duration of this hypoxemia. Fifty-four consecutive patients of both sexes, scheduled for elective jejunoileal bypass for morbid exogenous obesity, were studied. In 23 patients the operation was performed through a transverse incision and in 25 patients through a vertical incision. In 6 patients, cholecystectomy was also performed in addition to the jejunoileal bypass. Postoperatively there was a significant fall in PAO2 both in the transverse and vertical incision groups and this continued through day 4. On day 5 statistically significant hypoxemia was present only in the vertical incision group. In addition, the decrease in PaO2 was significantly greater on days 4 and 5 in the vertical group than in the transverse group. On days 2 and 3 again the mean decrease in PaO2 was greater in the vertical group but this was not statistically significant. The effect of added cholecystectomy on PaO2 was studied in 6 women with midline incisions. The results were compared with 6 subjects who had small bowel bypass without cholecystectomy and who were matched for incision, age weight, girth/height ratio, weight/height ratio, smoking habit, and percentage of predicted ERV. In both groups there was a significant fall of PAO2 from day 1 through day 4, but there was no significant difference between the two groups. Although the numbers studied were small, these limited results would suggest that the type of incesion rather than the intraoperative procedure is the important factor in determining postoperative PAO2 changes. These studies also suggest that in patients with an increased potential for postoperative hypoxemia (i.e., markedly obese), the transverse abdominal approach shoudl always be seriously considered.", "contents": "Choice of abdominal operative incision in the obese patient: a study using blood gas measurements. Although many factors may influence the choice of the operative incision, physiologic safety in  terms of the degree of postoperative hypoxemia should be considered. No data currently exist to delineate in markedly obese patients the effect of the operative incision on the extent or duration of this hypoxemia. Fifty-four consecutive patients of both sexes, scheduled for elective jejunoileal bypass for morbid exogenous obesity, were studied. In 23 patients the operation was performed through a transverse incision and in 25 patients through a vertical incision. In 6 patients, cholecystectomy was also performed in addition to the jejunoileal bypass. Postoperatively there was a significant fall in PAO2 both in the transverse and vertical incision groups and this continued through day 4. On day 5 statistically significant hypoxemia was present only in the vertical incision group. In addition, the decrease in PaO2 was significantly greater on days 4 and 5 in the vertical group than in the transverse group. On days 2 and 3 again the mean decrease in PaO2 was greater in the vertical group but this was not statistically significant. The effect of added cholecystectomy on PaO2 was studied in 6 women with midline incisions. The results were compared with 6 subjects who had small bowel bypass without cholecystectomy and who were matched for incision, age weight, girth/height ratio, weight/height ratio, smoking habit, and percentage of predicted ERV. In both groups there was a significant fall of PAO2 from day 1 through day 4, but there was no significant difference between the two groups. Although the numbers studied were small, these limited results would suggest that the type of incesion rather than the intraoperative procedure is the important factor in determining postoperative PAO2 changes. These studies also suggest that in patients with an increased potential for postoperative hypoxemia (i.e., markedly obese), the transverse abdominal approach shoudl always be seriously considered."}
{"id": "PMID:1094971", "title": "The hepatorenal syndrome: recovery after portacaval shunt.", "content": "Reversal of the morbid hepato-renal syndrome has been achieved in a cirrhotic patient with ascites following successful side-to-side portacaval shunt. The hepatorenal syndrome is defined as progressive unresponsive renal failure with previously normal kidneys in the presence of impaired hepatic function. Although the etiologic mechanism has not been defined, it is suggested the relationship of increased intrahepatic sinusoidal pressure on the thoracic duct and subsequent decreased lymph flow are interrelated to increased levels of aldosterone and manifested by (chylous) ascites. Laboratory and clinical evidence suggest that cirrhotics with ascites have remarkably high levels of aldosterone secretion via the rennin-angiotensin-adrenal cortex mechanism. This is the group that develops hepatorenal syndrome. Reduction of the intrahepatic pressure and decompression of the portal hypertension can be successfully achieved with a side-to-side shunt which should return the aldosterone-rennin-angiotensin axis to normal and subsequently reverse the hepatorenal syndrome.", "contents": "The hepatorenal syndrome: recovery after portacaval shunt. Reversal of the morbid hepato-renal syndrome has been achieved in a cirrhotic patient with ascites following successful side-to-side portacaval shunt. The hepatorenal syndrome is defined as progressive unresponsive renal failure with previously normal kidneys in the presence of impaired hepatic function. Although the etiologic mechanism has not been defined, it is suggested the relationship of increased intrahepatic sinusoidal pressure on the thoracic duct and subsequent decreased lymph flow are interrelated to increased levels of aldosterone and manifested by (chylous) ascites. Laboratory and clinical evidence suggest that cirrhotics with ascites have remarkably high levels of aldosterone secretion via the rennin-angiotensin-adrenal cortex mechanism. This is the group that develops hepatorenal syndrome. Reduction of the intrahepatic pressure and decompression of the portal hypertension can be successfully achieved with a side-to-side shunt which should return the aldosterone-rennin-angiotensin axis to normal and subsequently reverse the hepatorenal syndrome."}
{"id": "PMID:1094972", "title": "Strike-through of moist contamination by woven and nonwoven surgical materials.", "content": "A test is described which correlates the stress of stretching surgical gown and drape material with moist bacterial strike-through. By application of this test to a number of woven and nonwoven surgical gown and drape materials, it was found that not all of these materials, either woven or nonwoven, are impermeable to moist contamination for equal periods of time. Nonwoven disposable materials now in use range from those which remain impermeable to moist bacterial permeation through all tests while some remain impermeable for limited periods of time, and others almost immediately permeable to moist bacterial penetration. The same situation holds for woven materials. Under conditions of our test, Quarpel treated Pima tight-woven cotton cloth was impermeable to moist bacterial strike-through, through up to 75 washing and sterilizing cyclings, while ordinary linen and untreated Pima cloth permitted bacterial permeation almost immediately. These results have significance in lengthy wet surgical operations.", "contents": "Strike-through of moist contamination by woven and nonwoven surgical materials. A test is described which correlates the stress of stretching surgical gown and drape material with moist bacterial strike-through. By application of this test to a number of woven and nonwoven surgical gown and drape materials, it was found that not all of these materials, either woven or nonwoven, are impermeable to moist contamination for equal periods of time. Nonwoven disposable materials now in use range from those which remain impermeable to moist bacterial permeation through all tests while some remain impermeable for limited periods of time, and others almost immediately permeable to moist bacterial penetration. The same situation holds for woven materials. Under conditions of our test, Quarpel treated Pima tight-woven cotton cloth was impermeable to moist bacterial strike-through, through up to 75 washing and sterilizing cyclings, while ordinary linen and untreated Pima cloth permitted bacterial permeation almost immediately. These results have significance in lengthy wet surgical operations."}
{"id": "PMID:1094973", "title": "A universally applicable continuous suture technique for insertion of aortic valve prostheses.", "content": "In a series of 55 consecutive patients with both single- and multiple-valve disease and aortic aneurysm, the aortic valve was replaced using a continuous suture technique. The technique is applicable for both ball- and disc-valve prostheses. This method resulted in a marked reduction in cardiopulmonary bypass time with a very low incidence of perivalvular leak. It has major advantages over other continuous and interrupted suture techniques.", "contents": "A universally applicable continuous suture technique for insertion of aortic valve prostheses. In a series of 55 consecutive patients with both single- and multiple-valve disease and aortic aneurysm, the aortic valve was replaced using a continuous suture technique. The technique is applicable for both ball- and disc-valve prostheses. This method resulted in a marked reduction in cardiopulmonary bypass time with a very low incidence of perivalvular leak. It has major advantages over other continuous and interrupted suture techniques."}
{"id": "PMID:1094975", "title": "[Research on the effectiveness of gynecological prescreening (author's transl)].", "content": "Malignant tumors are determined by abnormal values of DNA as well as in its precursors. The cytophotometric analysis of smears allowed the determination of DNA of higher number of cells and therefore was presented as prescreening. But higher amounts of DNA caused by colpitis was reached too in this case. Therefore the presented research tried to limit points of malignancy by determination ofDNA, RNA, Histones, and Heterochromatin of cytologically classified cells. The discussion of results with literature pointed out, that this object is only reaching today by simultaneous determination of DNA and Heterochromatin of selected \"atypical\" cells.", "contents": "[Research on the effectiveness of gynecological prescreening (author's transl)]. Malignant tumors are determined by abnormal values of DNA as well as in its precursors. The cytophotometric analysis of smears allowed the determination of DNA of higher number of cells and therefore was presented as prescreening. But higher amounts of DNA caused by colpitis was reached too in this case. Therefore the presented research tried to limit points of malignancy by determination ofDNA, RNA, Histones, and Heterochromatin of cytologically classified cells. The discussion of results with literature pointed out, that this object is only reaching today by simultaneous determination of DNA and Heterochromatin of selected \"atypical\" cells."}
{"id": "PMID:1094976", "title": "[Group B-streptococci in the female genitals (author's transl)].", "content": "Swabs taken from the vagina, cervix and urethra of 152 women without any clinical signs of infection were studied for the presence of group B streptococci. 36.8% of these women were found to have group B streptococci in their genital tract. Isolation of streptococci took place by way of enrichment and selective culturing, determination of biochemical criteria as well as serological grouping and typing. Streptococci of human or animal origin which may be identified by precipitating group B antiserum were found to be identical. There were no criteria for a differentiation between human and animal (bovine) group B strains. Epidemiological associations between human infections and the consumption of raw milk containing galt streptococci have never been confirmed. Group B streptococci are present indepently in animals and humans. In humans, they may be present as saprophytes in the throat and female genitals and also cause severe disease like endocarditis and meningitis. There is no proof of the etiologic role as an agent of zooanthroponoses.", "contents": "[Group B-streptococci in the female genitals (author's transl)]. Swabs taken from the vagina, cervix and urethra of 152 women without any clinical signs of infection were studied for the presence of group B streptococci. 36.8% of these women were found to have group B streptococci in their genital tract. Isolation of streptococci took place by way of enrichment and selective culturing, determination of biochemical criteria as well as serological grouping and typing. Streptococci of human or animal origin which may be identified by precipitating group B antiserum were found to be identical. There were no criteria for a differentiation between human and animal (bovine) group B strains. Epidemiological associations between human infections and the consumption of raw milk containing galt streptococci have never been confirmed. Group B streptococci are present indepently in animals and humans. In humans, they may be present as saprophytes in the throat and female genitals and also cause severe disease like endocarditis and meningitis. There is no proof of the etiologic role as an agent of zooanthroponoses."}
{"id": "PMID:1094992", "title": "Amino acid residues of serum and CSF protein in multiple sclerosis. Clinical application of statistical discriminant analysis.", "content": "Statistical discriminant analysis of the amino acid compostion of serum and cerebrospinal fluid (CSF) proteins provides an objective method for distinguishing between normal controls and patients with multiple sclerosis (MS). This method also results in a high degree of specificity in separating MS patients from those with other diseases of the nervous system. The CSF protein serine residue is highly correlated with the CSF IgG and holds promise for a more sensitive diagnostic test for MS than the currently used CSF IgG. Finally, the serum/CSF protein serine ratio seems to correlate best with clinically determined degree of activity for the disease, the most active cases having the lowest ratio. These results suggest that investigation of the amino acid composition of serum and CSF protein in multiple sclerosis and, possibly, in other diseases might lead to the development of clinically useful tests of diagnosis and degree of activity of MS.", "contents": "Amino acid residues of serum and CSF protein in multiple sclerosis. Clinical application of statistical discriminant analysis. Statistical discriminant analysis of the amino acid compostion of serum and cerebrospinal fluid (CSF) proteins provides an objective method for distinguishing between normal controls and patients with multiple sclerosis (MS). This method also results in a high degree of specificity in separating MS patients from those with other diseases of the nervous system. The CSF protein serine residue is highly correlated with the CSF IgG and holds promise for a more sensitive diagnostic test for MS than the currently used CSF IgG. Finally, the serum/CSF protein serine ratio seems to correlate best with clinically determined degree of activity for the disease, the most active cases having the lowest ratio. These results suggest that investigation of the amino acid composition of serum and CSF protein in multiple sclerosis and, possibly, in other diseases might lead to the development of clinically useful tests of diagnosis and degree of activity of MS."}
{"id": "PMID:1094993", "title": "Acquired toxoplasmosis. A neglected cause of treatable nervous system disease.", "content": "The neurological manifestations of six cases of acquired central nervous system toxoplasmosis are compared with the 39 well-documented cases from the literature. Half of the patients had underlying systemic diseases (18 malignant neoplasms, two renal transplants, three collagen vascular diseases) treated with intensive immunosuppressive therapy. The remainder had primary toxoplasmosis. Three major neurological patterns were seen: (1) diffuse encephalopathy with or without seizures, (2) meningoencephalitis, and (3) singular or multiple progressive mass lesions. Routine neurological diagnostic studies were not helpful. The Sabin-Feldman dye test or IgM indirect fluorescent antibody test or both were effective in confirming the diagnosis. Twenty-seven patients died without a clinical diagnosis of toxoplasmosis. The diagnosis was made terminally in four additional patients. Thirteen of fourteen patients who received a full course of sulfadiazine or pyrimethamine or both did well. Toxoplasmosis should be considered in the immunosuppressed patient who appears with neurological involvement.", "contents": "Acquired toxoplasmosis. A neglected cause of treatable nervous system disease. The neurological manifestations of six cases of acquired central nervous system toxoplasmosis are compared with the 39 well-documented cases from the literature. Half of the patients had underlying systemic diseases (18 malignant neoplasms, two renal transplants, three collagen vascular diseases) treated with intensive immunosuppressive therapy. The remainder had primary toxoplasmosis. Three major neurological patterns were seen: (1) diffuse encephalopathy with or without seizures, (2) meningoencephalitis, and (3) singular or multiple progressive mass lesions. Routine neurological diagnostic studies were not helpful. The Sabin-Feldman dye test or IgM indirect fluorescent antibody test or both were effective in confirming the diagnosis. Twenty-seven patients died without a clinical diagnosis of toxoplasmosis. The diagnosis was made terminally in four additional patients. Thirteen of fourteen patients who received a full course of sulfadiazine or pyrimethamine or both did well. Toxoplasmosis should be considered in the immunosuppressed patient who appears with neurological involvement."}
{"id": "PMID:1094994", "title": "Chloramphenicol in aqueous humor after topical application.", "content": "Chloramphenicol 0.5% ophthalmic solution was applied topically to patients at various times before cataract surgery. The aqueous humor was obtained at the time of surgery and analyzed for chloramphenicol content by thin-layer chromatography. Aqueous humor chloramphenicol levels ranged from 3.5mn-g/ml to 6.7mn-g/ml at the initial sampling period one to two hours following topical administration. Chloramphenicol was measurable in samples for up to five hours following administration. The compound measured in the aqueous humor samples was chemically identified as intact chloramphenicol, and no metabolites of chloramphenicol were shown.", "contents": "Chloramphenicol in aqueous humor after topical application. Chloramphenicol 0.5% ophthalmic solution was applied topically to patients at various times before cataract surgery. The aqueous humor was obtained at the time of surgery and analyzed for chloramphenicol content by thin-layer chromatography. Aqueous humor chloramphenicol levels ranged from 3.5mn-g/ml to 6.7mn-g/ml at the initial sampling period one to two hours following topical administration. Chloramphenicol was measurable in samples for up to five hours following administration. The compound measured in the aqueous humor samples was chemically identified as intact chloramphenicol, and no metabolites of chloramphenicol were shown."}
{"id": "PMID:1094995", "title": "Antigenicity of cryopreserved corneas.", "content": "The antigenicity of fresh and cryopreserved corneal tissue was analyzed by performing 5-mm interlamellar xenografts in albino rabbits using dog corneas as the donor tissue. The cryopreservative solutions contained 10% rabbit albumin, dimethyl sulfoxide, and sucrose. Fifty-nine rabbits received interlamellar xenografts using donor tissue prepared by one of the three following methods: (1) fresh, (2) cryopreserved, and (3) immersed in the cryopreservative solutions, but not frozen. Approximately one third of the grafts became cloudy within six weeks, and no statistically significant difference was noted clinically or histologically between the three preparations of donor tissue used. The results indicate that cryopreservation has no significant effect on the antigenicity of corneal tissue as exemplified by the interlamellar xenograft reaction in rabbits.", "contents": "Antigenicity of cryopreserved corneas. The antigenicity of fresh and cryopreserved corneal tissue was analyzed by performing 5-mm interlamellar xenografts in albino rabbits using dog corneas as the donor tissue. The cryopreservative solutions contained 10% rabbit albumin, dimethyl sulfoxide, and sucrose. Fifty-nine rabbits received interlamellar xenografts using donor tissue prepared by one of the three following methods: (1) fresh, (2) cryopreserved, and (3) immersed in the cryopreservative solutions, but not frozen. Approximately one third of the grafts became cloudy within six weeks, and no statistically significant difference was noted clinically or histologically between the three preparations of donor tissue used. The results indicate that cryopreservation has no significant effect on the antigenicity of corneal tissue as exemplified by the interlamellar xenograft reaction in rabbits."}
{"id": "PMID:1094996", "title": "An automatic objective optometer. Description and clinical evaluation.", "content": "Automatic objective optometers provide a possible alternative to conventional clinical methods of refracting the human eye. Quantitative evaluation of one of these instruments (Dioptron), including a comparison between optometer and subjective refractions, was undertaken to determine how automatic optometers may be utilized most effectively in clinical situations. The results show a high degree of instrument validity and reliability. The conclusion is that the Dioptron has substantial promise as a means of estimating ametropia-as a replacement for retinoscopy and for screening or monitoring ametropia. However, the use of this instrument as a substitute for subjective refraction procedures does not seem warranted at this time.", "contents": "An automatic objective optometer. Description and clinical evaluation. Automatic objective optometers provide a possible alternative to conventional clinical methods of refracting the human eye. Quantitative evaluation of one of these instruments (Dioptron), including a comparison between optometer and subjective refractions, was undertaken to determine how automatic optometers may be utilized most effectively in clinical situations. The results show a high degree of instrument validity and reliability. The conclusion is that the Dioptron has substantial promise as a means of estimating ametropia-as a replacement for retinoscopy and for screening or monitoring ametropia. However, the use of this instrument as a substitute for subjective refraction procedures does not seem warranted at this time."}
{"id": "PMID:1094997", "title": "Polyglycolic acid suture in strabismus surgery.", "content": "Polyglycolic acid, synthetic, absorbable braided suture was compared to gut with respect to handling, strength, and tissue reaction in 70 strabismus procedures. This new suture material produced less tissue reaction than gut and had handling characteristics that were similar to silk. It can be used in smaller sizes than gut because of its greater strength and firm knot. Polyglycolic acid suture appears to be a useful addition to ophthalmic suture materials.", "contents": "Polyglycolic acid suture in strabismus surgery. Polyglycolic acid, synthetic, absorbable braided suture was compared to gut with respect to handling, strength, and tissue reaction in 70 strabismus procedures. This new suture material produced less tissue reaction than gut and had handling characteristics that were similar to silk. It can be used in smaller sizes than gut because of its greater strength and firm knot. Polyglycolic acid suture appears to be a useful addition to ophthalmic suture materials."}
{"id": "PMID:1094998", "title": "[Diagnostic problems in new and old injuries].", "content": "Due to mass participation in certain sports combination injuries of the knee joint have greatly increased in number. To a certain extend injuries of the cruciate ligaments are typical. According to our own follow-up studies the immidiate suture of the ligaments leads to better results than late reconstructions which emphasises the early diagnosis of an injury to the cruciate ligaments. Old and new injuries to these ligaments present diagnostic problems. Therefore the mechanism of injury, the symptomatology, and both clinical and radiological possibilities for the diagnosis of damage to the cruciate ligaments are demonstrated.", "contents": "[Diagnostic problems in new and old injuries]. Due to mass participation in certain sports combination injuries of the knee joint have greatly increased in number. To a certain extend injuries of the cruciate ligaments are typical. According to our own follow-up studies the immidiate suture of the ligaments leads to better results than late reconstructions which emphasises the early diagnosis of an injury to the cruciate ligaments. Old and new injuries to these ligaments present diagnostic problems. Therefore the mechanism of injury, the symptomatology, and both clinical and radiological possibilities for the diagnosis of damage to the cruciate ligaments are demonstrated."}
{"id": "PMID:1095010", "title": "Carbohydrate absorption. Studies on the glucose transport by isolated brush border membranes. A contribution towards an understanding of the molecular mechanism of sugar absorption.", "content": "From investigations, mainly on intact in vitro preparations of small intestine, it has been established that the epithelial cell carries out digestion as well as absorption of carbohydrates, i.e. the luminal (brush border) plasma membrane contains di- and poly-saccharidases for the degradation of carbohydrates to monosaccharides as well as transport systems for monosaccharides. Recently, an intact glucose transport system has been demonstrated in isolated membranes from rat intestinal brush borders. In the isolated membranes, glucose transport has the characteristics expected for 'facilitated diffusion' of glucose coupled to Na+ translocation. The Na+-dependent uptake of glucose can be influenced by electrical potentials across the brush border membrane, which indicates that the positive charge of Na+, associated with sugar uptake, is not compensated by the counter-movement of another cation, e.g. K+, or by the co-movement of an anion via the same glucose 'carrier'. In other words, the Na+-coupled D-glucose transport is linked to a charge translocation - it is electrogenic. The apparent Na+ independence of glucose transport in vivo and the accumulation of glucose against a glucose as well as a Na+ concentration gradient by isolated epithelial cells may be explained by the 'ion gradient' model of Crane and Schultz and Zalusky provided the electrical membrane potential is high under these conditions and the electrochemical potential of Na+ serves as driving force for 'active' glucose transport.", "contents": "Carbohydrate absorption. Studies on the glucose transport by isolated brush border membranes. A contribution towards an understanding of the molecular mechanism of sugar absorption. From investigations, mainly on intact in vitro preparations of small intestine, it has been established that the epithelial cell carries out digestion as well as absorption of carbohydrates, i.e. the luminal (brush border) plasma membrane contains di- and poly-saccharidases for the degradation of carbohydrates to monosaccharides as well as transport systems for monosaccharides. Recently, an intact glucose transport system has been demonstrated in isolated membranes from rat intestinal brush borders. In the isolated membranes, glucose transport has the characteristics expected for 'facilitated diffusion' of glucose coupled to Na+ translocation. The Na+-dependent uptake of glucose can be influenced by electrical potentials across the brush border membrane, which indicates that the positive charge of Na+, associated with sugar uptake, is not compensated by the counter-movement of another cation, e.g. K+, or by the co-movement of an anion via the same glucose 'carrier'. In other words, the Na+-coupled D-glucose transport is linked to a charge translocation - it is electrogenic. The apparent Na+ independence of glucose transport in vivo and the accumulation of glucose against a glucose as well as a Na+ concentration gradient by isolated epithelial cells may be explained by the 'ion gradient' model of Crane and Schultz and Zalusky provided the electrical membrane potential is high under these conditions and the electrochemical potential of Na+ serves as driving force for 'active' glucose transport."}
{"id": "PMID:1095012", "title": "Vitamin A a absorption - nutritional aspects.", "content": "A brief review of the present knowledge of vitamin A (both performed and precursor forms) absorption is outlined with special emphasis on the dietary factors involved in this process. Some details are discussed related to the techniques used for measurement of vitamin A absorption from different sources and under different experimental conditions. Suggestions are also made to standardize appropriate procedure in this respect; this might enable comparisons and wide use of respective results obtained in various laboratories. Nutritional significance, including advantages or disadvantages of the liver test in these studies, is specifically highlighted with special reference to the determination of vitamin A bio-potency in various products. Some selected results and experience in this field are briefly presented.", "contents": "Vitamin A a absorption - nutritional aspects. A brief review of the present knowledge of vitamin A (both performed and precursor forms) absorption is outlined with special emphasis on the dietary factors involved in this process. Some details are discussed related to the techniques used for measurement of vitamin A absorption from different sources and under different experimental conditions. Suggestions are also made to standardize appropriate procedure in this respect; this might enable comparisons and wide use of respective results obtained in various laboratories. Nutritional significance, including advantages or disadvantages of the liver test in these studies, is specifically highlighted with special reference to the determination of vitamin A bio-potency in various products. Some selected results and experience in this field are briefly presented."}
{"id": "PMID:1095014", "title": "Overproduction of lysine by mutant strains of Escherichia coli with defective lysine transport systems.", "content": "Mutants selected on the basis of their resistance to S-(beta-aminoethyl) cysteine and overproduction of lysine were found to be defective in the lysine transport system. The overproduction of lysine was not due to mutation affecting either of the two regulatory enzymes aspartokinase and dihydrodipicolinic acid synthetase. Uptake of labeled lysine by the lysine-specific transport system was reduced to a negligible level, while uptake by the lysine, ornithine, arginine system was also affected. A hypothesis regarding the nature of these mutations and their effects on the regulation of lysine biosynthesis is discussed.", "contents": "Overproduction of lysine by mutant strains of Escherichia coli with defective lysine transport systems. Mutants selected on the basis of their resistance to S-(beta-aminoethyl) cysteine and overproduction of lysine were found to be defective in the lysine transport system. The overproduction of lysine was not due to mutation affecting either of the two regulatory enzymes aspartokinase and dihydrodipicolinic acid synthetase. Uptake of labeled lysine by the lysine-specific transport system was reduced to a negligible level, while uptake by the lysine, ornithine, arginine system was also affected. A hypothesis regarding the nature of these mutations and their effects on the regulation of lysine biosynthesis is discussed."}
{"id": "PMID:1095019", "title": "Antilymphocyte antibodies in systemic lupus erythematosus. Effect on lymphocyte surface characteristics.", "content": "Antilymphocyte antibodies in SLE bind to normal lymphocytes and are not always associated with lymphocytotoxic activity. Detection of these antibodies on the surface of normal lymphocytes by immunofluorescent techniques is enhanced by short incubation periods, reduced temperature, and metabolic inhibition. The same effects occur in SLE blood and may influence the enumeration of B lymphocytes as determined by surface immunoglobulin. The percent of T lymphocytes forming sheep erythrocyte rosettes is not affected by these antibodies.", "contents": "Antilymphocyte antibodies in systemic lupus erythematosus. Effect on lymphocyte surface characteristics. Antilymphocyte antibodies in SLE bind to normal lymphocytes and are not always associated with lymphocytotoxic activity. Detection of these antibodies on the surface of normal lymphocytes by immunofluorescent techniques is enhanced by short incubation periods, reduced temperature, and metabolic inhibition. The same effects occur in SLE blood and may influence the enumeration of B lymphocytes as determined by surface immunoglobulin. The percent of T lymphocytes forming sheep erythrocyte rosettes is not affected by these antibodies."}
{"id": "PMID:1095020", "title": "Depression of cellular-mediated immunity in systemic lupus erythematosus. relation to disease activity.", "content": "Cellular-mediated immunity was examined in 6 consecutive patients with active SLE, 6 patients with low active SLE 6 patients with DLE, 8 patients with other collagen diseases, and 9 healthy controls. The following parameters were measured: delayed hypersensitivity, in vitro lymphocyte blast transformation, and MIF production after stimulation with five common antigens, DNA, and two nonspecific mitogens (PHA, PWM). The 6 active SLE patients were anergic and had a markedly depressed response in vitro to the specific antigens and PWM (P less than 0.001). When CMI was retested in 5 patients as early as 5 days after the start of prednisone (60 mc/day), the results were significantly improved in 4 who also improved clinically, and they remained unchanged in a fifth patieht who failed to improve. There was a less marked impairment of CMI among the low active SLE group, whereas the DLE and OCD patients did not differ significantly from the control subjects. DNA did not cause significant stimulation of blast transformation in any of the study groups.", "contents": "Depression of cellular-mediated immunity in systemic lupus erythematosus. relation to disease activity. Cellular-mediated immunity was examined in 6 consecutive patients with active SLE, 6 patients with low active SLE 6 patients with DLE, 8 patients with other collagen diseases, and 9 healthy controls. The following parameters were measured: delayed hypersensitivity, in vitro lymphocyte blast transformation, and MIF production after stimulation with five common antigens, DNA, and two nonspecific mitogens (PHA, PWM). The 6 active SLE patients were anergic and had a markedly depressed response in vitro to the specific antigens and PWM (P less than 0.001). When CMI was retested in 5 patients as early as 5 days after the start of prednisone (60 mc/day), the results were significantly improved in 4 who also improved clinically, and they remained unchanged in a fifth patieht who failed to improve. There was a less marked impairment of CMI among the low active SLE group, whereas the DLE and OCD patients did not differ significantly from the control subjects. DNA did not cause significant stimulation of blast transformation in any of the study groups."}
{"id": "PMID:1095021", "title": "Lymphokines in the rheumatoid joint.", "content": "Joint fluids and culture supernatants of synovial tissue were examined for the presence of two lymphokines. Migration inhibitory activity was found in 16 of 22 rheumatoid fluids (73%), in 3 of 15 osteoarthritis fluids (20%), and in 3 of 11 fluids from patients with various inflammatory arthritides (27%). Blastogenic activity was present in 14 of 15 rheumatoid fluids and in 1 of 7 nonrheumatoid effusions. The active materials eluted with the third peak from Sephadex G-200 and were therefore smaller than immunoglobulin or immune complexes. These findings suggest that lymphokine-like substances were present in effusions and synovial tissue of most patients with rheumatoid arthritis and some patients with other forms of chronic synovitis.", "contents": "Lymphokines in the rheumatoid joint. Joint fluids and culture supernatants of synovial tissue were examined for the presence of two lymphokines. Migration inhibitory activity was found in 16 of 22 rheumatoid fluids (73%), in 3 of 15 osteoarthritis fluids (20%), and in 3 of 11 fluids from patients with various inflammatory arthritides (27%). Blastogenic activity was present in 14 of 15 rheumatoid fluids and in 1 of 7 nonrheumatoid effusions. The active materials eluted with the third peak from Sephadex G-200 and were therefore smaller than immunoglobulin or immune complexes. These findings suggest that lymphokine-like substances were present in effusions and synovial tissue of most patients with rheumatoid arthritis and some patients with other forms of chronic synovitis."}
{"id": "PMID:1095022", "title": "Selective suppression of autoantibody responses in NZB/NZW mice treated with long-term cyclophosphamide.", "content": "Autoimmune responses were assayed in 80 cyclophosphamide-treated and control NZB/NZW mice over a period of 1 year. Fluctuation between positive and negative immunofluorescent heterogeneous ANA tests and daily alterations of ANA titers were detected in young mice of both sexes. Although high-dose cyclophosphamide therapy (8 mg/kg/day) failed to prevent the spontaneous appearance of ANA, titered ANA values were partially suppressed in high-dose treated mice. This study permitted sequential comparisons between ANA titers and anti-DNA as useful indices of cyclophosphamide-induced suppression of autoimmune disease. ANA titers were relatively resistant to cyclophosphamide therapy. Antibodies directed specifically against DNA were suppressed mice receiving high-dose cyclophosphamide. In treated animals, decreased anti-DNA levels were associated with protection from severe glomerulonephritis and renal vasculitis. Treatment with low-dose cyclophosphamide (1 mg/kg/day) appeared paradoxically to stimulate autoantibody production and renal disease/vasculitis.", "contents": "Selective suppression of autoantibody responses in NZB/NZW mice treated with long-term cyclophosphamide. Autoimmune responses were assayed in 80 cyclophosphamide-treated and control NZB/NZW mice over a period of 1 year. Fluctuation between positive and negative immunofluorescent heterogeneous ANA tests and daily alterations of ANA titers were detected in young mice of both sexes. Although high-dose cyclophosphamide therapy (8 mg/kg/day) failed to prevent the spontaneous appearance of ANA, titered ANA values were partially suppressed in high-dose treated mice. This study permitted sequential comparisons between ANA titers and anti-DNA as useful indices of cyclophosphamide-induced suppression of autoimmune disease. ANA titers were relatively resistant to cyclophosphamide therapy. Antibodies directed specifically against DNA were suppressed mice receiving high-dose cyclophosphamide. In treated animals, decreased anti-DNA levels were associated with protection from severe glomerulonephritis and renal vasculitis. Treatment with low-dose cyclophosphamide (1 mg/kg/day) appeared paradoxically to stimulate autoantibody production and renal disease/vasculitis."}
{"id": "PMID:1095025", "title": "[Treatment of inflammatory complications of permanent implanted pacemakers].", "content": "The Authors report an experience of 165 patients who underwent to implantation of artificial pacemaker from January 1973 to December 1974 and report 16 cases of infection. They consider many surgical and biologic factors that can have caused uprinsing of infection. The Authors report the therapeutic treatment the patients underwent and conclude that the best treatment is the implantation, to another seat, of new pacemaker.", "contents": "[Treatment of inflammatory complications of permanent implanted pacemakers]. The Authors report an experience of 165 patients who underwent to implantation of artificial pacemaker from January 1973 to December 1974 and report 16 cases of infection. They consider many surgical and biologic factors that can have caused uprinsing of infection. The Authors report the therapeutic treatment the patients underwent and conclude that the best treatment is the implantation, to another seat, of new pacemaker."}
{"id": "PMID:1095026", "title": "[Clinical significance of changes in tryptophan metabolism].", "content": "The Authors describe the various anomalies of the metabolism of tryptophan that are observed in various diseases. The oxidative pathway is most important of the metabolic pathway of the amino acid; the degredation of tryptophan is particularly influenced by steroid hormones and vitamins' want. The metabolic anomalies are demonstrable both in malignant tumors (mostly in bladder cancer and Hodgkin's disease), both during psychiatric diseases (such as depression and schizophrenia) and in the diseases of connective tissue in addition to congenital errors of the degradation of tryptophan (such as Hartnup's disease, tryptophanuria and 3-hydroxychinureninuria). The metabolic pictures are manifest after amino acid's in the diseases of connective tissue but are independent for clinical seriousness and, in any case, less significant than those observed in other pathological pictures, mostly in Hodgkin's disease. The existence of anomalies of tryptophan's metabolism is certainly shown in many diseases, however the true physiopathogenetic meaning of these metabolic alterations is not yet specified. Particularly it is not definite if these alterations are the cause of diseases, which they appear in, or if they are secondary alterations.", "contents": "[Clinical significance of changes in tryptophan metabolism]. The Authors describe the various anomalies of the metabolism of tryptophan that are observed in various diseases. The oxidative pathway is most important of the metabolic pathway of the amino acid; the degredation of tryptophan is particularly influenced by steroid hormones and vitamins' want. The metabolic anomalies are demonstrable both in malignant tumors (mostly in bladder cancer and Hodgkin's disease), both during psychiatric diseases (such as depression and schizophrenia) and in the diseases of connective tissue in addition to congenital errors of the degradation of tryptophan (such as Hartnup's disease, tryptophanuria and 3-hydroxychinureninuria). The metabolic pictures are manifest after amino acid's in the diseases of connective tissue but are independent for clinical seriousness and, in any case, less significant than those observed in other pathological pictures, mostly in Hodgkin's disease. The existence of anomalies of tryptophan's metabolism is certainly shown in many diseases, however the true physiopathogenetic meaning of these metabolic alterations is not yet specified. Particularly it is not definite if these alterations are the cause of diseases, which they appear in, or if they are secondary alterations."}
{"id": "PMID:1095027", "title": "[Solute charge in milk feeding of babies with diarrhea].", "content": "In each one of the samples, investigation of osmolarity, concentration of sodium chloride and proteins and bacteria was carried out. With these data, concentration of solutes in milks was estimated, together with a calculation of renal charge they represented. The most outstanding results showed that mothers offer whole and half skimmed milks at concentrations less than normal dilutions; thus, the solute charge was found in most cases, far below figures reported as average. In a good number of milks, osmolarity showed to very high, specially in those added with corn syrup. Only in 9 of the formulas germs were not identified; in the rest, E. coli predominated and there was high index of contamination.", "contents": "[Solute charge in milk feeding of babies with diarrhea]. In each one of the samples, investigation of osmolarity, concentration of sodium chloride and proteins and bacteria was carried out. With these data, concentration of solutes in milks was estimated, together with a calculation of renal charge they represented. The most outstanding results showed that mothers offer whole and half skimmed milks at concentrations less than normal dilutions; thus, the solute charge was found in most cases, far below figures reported as average. In a good number of milks, osmolarity showed to very high, specially in those added with corn syrup. Only in 9 of the formulas germs were not identified; in the rest, E. coli predominated and there was high index of contamination."}
{"id": "PMID:1095036", "title": "Contribution of cardiac pacing to our understanding of the Wolff-Parkinson-White syndrome.", "content": "This review discusses the information which can be obtained by cardiac pacing in patients with the Wolff-Parkinson-White syndrome. Programmed electrical stimulation when combined with the recording of intracardiac electrograms and surface electrocardiograph leads, can be extremely useful in the following areas. 1) Determining the type of the accessory atrioventricular connexions; 2) determining the electrophysiological properties of the accessory atrioventricular pathway; 3) localizing the position of the accessory atrioventricular pathway; 4) determining the mechanisms of any tachycardia; 5) assessing effect of drugs; 6) identifying patients likely to be at high risk; and 7)evaluating postoperative conduction.", "contents": "Contribution of cardiac pacing to our understanding of the Wolff-Parkinson-White syndrome. This review discusses the information which can be obtained by cardiac pacing in patients with the Wolff-Parkinson-White syndrome. Programmed electrical stimulation when combined with the recording of intracardiac electrograms and surface electrocardiograph leads, can be extremely useful in the following areas. 1) Determining the type of the accessory atrioventricular connexions; 2) determining the electrophysiological properties of the accessory atrioventricular pathway; 3) localizing the position of the accessory atrioventricular pathway; 4) determining the mechanisms of any tachycardia; 5) assessing effect of drugs; 6) identifying patients likely to be at high risk; and 7)evaluating postoperative conduction."}
{"id": "PMID:1095038", "title": "Death during operation.", "content": "Although deaths that take place during the course of an operation are commonly called \"Death under the Anaesthetic\", they did occur before the advent of anaesthesia and in 1824 Dupuytren listed seven posssible causes. Thirty-three reported cases are mentioned and compared with Dupuytren's list. Air embolism was the commonest cause. The last survey in 1956 suggested that anaesthesia was entirely responsible for about 60 per cent of cases and surgery for 10 per cent.", "contents": "Death during operation. Although deaths that take place during the course of an operation are commonly called \"Death under the Anaesthetic\", they did occur before the advent of anaesthesia and in 1824 Dupuytren listed seven posssible causes. Thirty-three reported cases are mentioned and compared with Dupuytren's list. Air embolism was the commonest cause. The last survey in 1956 suggested that anaesthesia was entirely responsible for about 60 per cent of cases and surgery for 10 per cent."}
{"id": "PMID:1095039", "title": "A comparison of methods for the measurement of cardiac output and blood oxygen content.", "content": "A comparison has been made between the recently introduced thermal dilution method for measurement of cardiac output and the standard dye dilution technique. Two relatively new methods of measurement of blood oxygen content, one involving the measurement of oxygen tension after release of oxygen by carbon monoxide, and the other the measurement of current flow upon reduction of oxygen in a galvanic cell, have been compared with a standard indirect method of measurement of blood oxygen content. All three new methods fulfilled the criteria of accuracy and simplicity and compared favourably with the standard methods. Of the two new methods for measurement of oxygen content, that involving reduction of oxygen in a galvanic cell was superior by virtue of compactness and speed of operation.", "contents": "A comparison of methods for the measurement of cardiac output and blood oxygen content. A comparison has been made between the recently introduced thermal dilution method for measurement of cardiac output and the standard dye dilution technique. Two relatively new methods of measurement of blood oxygen content, one involving the measurement of oxygen tension after release of oxygen by carbon monoxide, and the other the measurement of current flow upon reduction of oxygen in a galvanic cell, have been compared with a standard indirect method of measurement of blood oxygen content. All three new methods fulfilled the criteria of accuracy and simplicity and compared favourably with the standard methods. Of the two new methods for measurement of oxygen content, that involving reduction of oxygen in a galvanic cell was superior by virtue of compactness and speed of operation."}
{"id": "PMID:1095040", "title": "Recovery and skills related to driving after intravenous sedation: dose-response relationship with diazepam.", "content": "Skills related to driving, the ability to discriminate the fusion of flickering light, and hand and foot proprioception, were measured double-blind in 34 healthy volunteers before and after three doses of i.v. diazepam. The effects of diazepam were most harmful to co-ordination. With 0.15 mg/kg, 0.30 mg/kg and 0.45 mg/kg of diazepam the impairment of co-ordinative skills was statistically significant (P less than 0.05) up to 2, 6 and 8 hr respectively. No impairment of performance on any test was measurable at 6 hr after 0.15 mg/kg or at 10 hr after 0.30 or 0.45 mg/kg of diazepam. There were large interindividual variations in serum concentrations of diazepam within each dose level. The increases in serum concentrations of diazepam after the intake of food support the concept of an enterohepatic cycle for diazepam. It was concluded that patients should not drive or operate machinery for at least 6 hr after 0.15 mg/kg of i.v. diazepam and at least 10 hr after 0.30 mg/kg and 0.45 mg/kg.", "contents": "Recovery and skills related to driving after intravenous sedation: dose-response relationship with diazepam. Skills related to driving, the ability to discriminate the fusion of flickering light, and hand and foot proprioception, were measured double-blind in 34 healthy volunteers before and after three doses of i.v. diazepam. The effects of diazepam were most harmful to co-ordination. With 0.15 mg/kg, 0.30 mg/kg and 0.45 mg/kg of diazepam the impairment of co-ordinative skills was statistically significant (P less than 0.05) up to 2, 6 and 8 hr respectively. No impairment of performance on any test was measurable at 6 hr after 0.15 mg/kg or at 10 hr after 0.30 or 0.45 mg/kg of diazepam. There were large interindividual variations in serum concentrations of diazepam within each dose level. The increases in serum concentrations of diazepam after the intake of food support the concept of an enterohepatic cycle for diazepam. It was concluded that patients should not drive or operate machinery for at least 6 hr after 0.15 mg/kg of i.v. diazepam and at least 10 hr after 0.30 mg/kg and 0.45 mg/kg."}
{"id": "PMID:1095046", "title": "A controlled trail of doxapram in acute respiratory failure.", "content": "The efficacy of a respiratory stimulant (doxapram) in antagonizing carbon dioxide retention associated with controlled oxygen therapy (28 per cent) in acute-on-chronic respiratory failure was assessed by a double-blind crossover trial in eight patients. PaCO2 rose in egery case during the placebo in the initial treatment period (mean increase 10 mm Hg). In those patients receiving doxapram initially PaCO2 fell in three and rose in one (mean decrease 4 mm Hg). All who has shown a rise in PaCO2 during the initial period on placebo showed a fall in the subsequent period on doxapram. In the six patients who completed the study, mean PaCO2 at the end of the doxapram period was significantly lower than the end of the placebo period (60 mm Hg against 67 mm M Hg). In three patients the rise in PaCO2 was accompanied by significant impairment in the level of consciousness which was reversed during the doxapram period. PaCO2 was not significantly different during either treatment period. No serious unwanted effects were encountered.", "contents": "A controlled trail of doxapram in acute respiratory failure. The efficacy of a respiratory stimulant (doxapram) in antagonizing carbon dioxide retention associated with controlled oxygen therapy (28 per cent) in acute-on-chronic respiratory failure was assessed by a double-blind crossover trial in eight patients. PaCO2 rose in egery case during the placebo in the initial treatment period (mean increase 10 mm Hg). In those patients receiving doxapram initially PaCO2 fell in three and rose in one (mean decrease 4 mm Hg). All who has shown a rise in PaCO2 during the initial period on placebo showed a fall in the subsequent period on doxapram. In the six patients who completed the study, mean PaCO2 at the end of the doxapram period was significantly lower than the end of the placebo period (60 mm Hg against 67 mm M Hg). In three patients the rise in PaCO2 was accompanied by significant impairment in the level of consciousness which was reversed during the doxapram period. PaCO2 was not significantly different during either treatment period. No serious unwanted effects were encountered."}
{"id": "PMID:1095049", "title": "An operation for restoration of urinary continence following total loss of the urethra.", "content": "Twenty-three patients with urethral loss have been treated by the simple creation ofa \"spout\" from the bladder wall. The procedure is suggested for the surgical treatment of lymphopathia venereum and for consideration in certain other conditions when urinary diversion is unacceptable.", "contents": "An operation for restoration of urinary continence following total loss of the urethra. Twenty-three patients with urethral loss have been treated by the simple creation ofa \"spout\" from the bladder wall. The procedure is suggested for the surgical treatment of lymphopathia venereum and for consideration in certain other conditions when urinary diversion is unacceptable."}
{"id": "PMID:1095050", "title": "The paretic pupil: its incidence and aetiology after keratoplasty for keratoconus.", "content": "The present study reveals that pupillary abnormalities are common after keratoplasty for keratoconus and that, in addition to the fixed dilated pupils which we have found in 7.8 per cent. of eyes, varying degrees of partially dilated pupil frequently occur after operation. In our experience, glaucoma is not a sequel to the simple paretic pupil, a finding which confirms the results of the smaller series of Alberth and Schnitzler (1971); glaucoma thus seems to be no more a special complication of keratoplasty for keratoconus than it is of keratoplasty for any other corneal pathology. The paretic pupils can be explained on the basis of ischaemic atrophy of the sphincter pupillae muscle secondary to an iris strangulation phenomenon occurring during surgery in the manner we have discussed. The relative frequency of a dilated pupil, together with the common finding of focal iris atrophy after minimal surgical trauma to the iris in cases of keratoconus, forces one to conclude that the pathology in this condition is not confined to the cornea but probably extends to the iris and possibly to the scleral envelope as well.", "contents": "The paretic pupil: its incidence and aetiology after keratoplasty for keratoconus. The present study reveals that pupillary abnormalities are common after keratoplasty for keratoconus and that, in addition to the fixed dilated pupils which we have found in 7.8 per cent. of eyes, varying degrees of partially dilated pupil frequently occur after operation. In our experience, glaucoma is not a sequel to the simple paretic pupil, a finding which confirms the results of the smaller series of Alberth and Schnitzler (1971); glaucoma thus seems to be no more a special complication of keratoplasty for keratoconus than it is of keratoplasty for any other corneal pathology. The paretic pupils can be explained on the basis of ischaemic atrophy of the sphincter pupillae muscle secondary to an iris strangulation phenomenon occurring during surgery in the manner we have discussed. The relative frequency of a dilated pupil, together with the common finding of focal iris atrophy after minimal surgical trauma to the iris in cases of keratoconus, forces one to conclude that the pathology in this condition is not confined to the cornea but probably extends to the iris and possibly to the scleral envelope as well."}
{"id": "PMID:1095051", "title": "Intravitreal hyaluronic acid injection. A long-term clinical evaluation.", "content": "Healon-H was used in the treatment of seven cases of complicated retinal detachment. A permanent re-attachment was achieved in two cases. No serious ocular complications have been encountered after a follow-up of 4 1/2 years.", "contents": "Intravitreal hyaluronic acid injection. A long-term clinical evaluation. Healon-H was used in the treatment of seven cases of complicated retinal detachment. A permanent re-attachment was achieved in two cases. No serious ocular complications have been encountered after a follow-up of 4 1/2 years."}
{"id": "PMID:1095053", "title": "Essential thiols of yeast hexokinase: alkylation by a substrate-like reagent.", "content": "It is demonstrated that N-bromoacetyl-D-galactosamine acts as a substrate-like reagent for yeast hexokinases A and B, producing affinity labeling. At the order of 10(-3) M reagent concentrations, rapid inactivation of the enzyme is produced: the kinetics are consistent with dependence upon a reversible inhibitor-enzyme initial complex, with a dissociation constant of 3.8 x 10(-3) M for hexokinase B at 35 degrees, pH 8.5. The glucose analog is 30-fold less effective, presumably due to self-protection. The inactivating reaction is an order of magnitude faster than that with bromoacetate. All the alkylation of hexokinase B was shown to occur at two thiol groups per subunit, associated stoichiometrically with inactivation. Unlike the reaction there of simple alkylators, two nonessential thiols per subunit are left unattacked when this inactivation reaction is complete. Protection against the affinity alkylation is exerted by the substrates glucose, mannose, fructose, glucose 6-phosphate, fructose 6-phosphate, ATP-Mg, and ADP-Mg, in proportion to their affinities for the active center. Free ATP also protects. Mg2+ alone has no influence, and Mn2+ gives a slight acceleration, when correction is made for a slow inactivation that occurs when the enzyme is incubated at 35 degrees with Mn2+ alone. Galactose, virtually a nonsubstrate, has no influence on the affinity alkylation, but N-acetylgalactosamine, a nonsubstrate and a weak inhibitor of the enzymic reaction, has an accelerating effect. An interpretation is made in terms of binding to a site that influences the active center. This affinity label should provide a means of isolating a peptide containing active-center-related groups.", "contents": "Essential thiols of yeast hexokinase: alkylation by a substrate-like reagent. It is demonstrated that N-bromoacetyl-D-galactosamine acts as a substrate-like reagent for yeast hexokinases A and B, producing affinity labeling. At the order of 10(-3) M reagent concentrations, rapid inactivation of the enzyme is produced: the kinetics are consistent with dependence upon a reversible inhibitor-enzyme initial complex, with a dissociation constant of 3.8 x 10(-3) M for hexokinase B at 35 degrees, pH 8.5. The glucose analog is 30-fold less effective, presumably due to self-protection. The inactivating reaction is an order of magnitude faster than that with bromoacetate. All the alkylation of hexokinase B was shown to occur at two thiol groups per subunit, associated stoichiometrically with inactivation. Unlike the reaction there of simple alkylators, two nonessential thiols per subunit are left unattacked when this inactivation reaction is complete. Protection against the affinity alkylation is exerted by the substrates glucose, mannose, fructose, glucose 6-phosphate, fructose 6-phosphate, ATP-Mg, and ADP-Mg, in proportion to their affinities for the active center. Free ATP also protects. Mg2+ alone has no influence, and Mn2+ gives a slight acceleration, when correction is made for a slow inactivation that occurs when the enzyme is incubated at 35 degrees with Mn2+ alone. Galactose, virtually a nonsubstrate, has no influence on the affinity alkylation, but N-acetylgalactosamine, a nonsubstrate and a weak inhibitor of the enzymic reaction, has an accelerating effect. An interpretation is made in terms of binding to a site that influences the active center. This affinity label should provide a means of isolating a peptide containing active-center-related groups."}
{"id": "PMID:1095054", "title": "Partial purification and properties of an enzyme from Escherichia coli that catalyzes the conversion of glutamic acid and 10-formyltetrahydropteroylglutamic acid to 10-formyltetrahydropterol-gamma-glutamyglutamic acid.", "content": "An enzyme that catalyzes the conversion of L-glutamic acid and 10-formyl-H4folic acid (also known as 10-formyl-H4pteroylglutamic acid) to 10-formyl-H4pteroyl-gamma-glutamylglutamic acid has been purified by 74-fold from extracts of Escherichia coli. ATP, Mg-2+, and a monovalent cation (K+ or NH-4, but not Na+) are required for the enzyme to function. Radioactive and bioautographic analyses revealed the formation of a single product. This product was identified as 10-formyl-H-4pteroyl-gamma-glutamylglutamic acid from its spectral characteristics, its ability to be used effectively as a growth faster for Lactobacillus casei 7469, and from radioactive analysis that indicated the incorporation into the product of 1 mol glutamate/mol of 10-formyl-H-4pteroylglutamic acid utilized. The enzyme functions optimally at pH 9.0-9.8 and at 50 degrees. Its molecular weight is estimated at 42,000-43,000. The Km values are 180 muM for L-glutamic acid and less than 2 muM for (-) 10-formyl-H-4pteroylglutamic acid. The only other naturally occurring folate compounds with significant activity as substrate are H-4pteroylglutamic acid and 5,10-methylene-H-4pteroylglutamic acid; however, these compounds are not used as effectively (K-m values are 10-12 mu-M) as 10-formyl-H-4pteroylglutamic acid.", "contents": "Partial purification and properties of an enzyme from Escherichia coli that catalyzes the conversion of glutamic acid and 10-formyltetrahydropteroylglutamic acid to 10-formyltetrahydropterol-gamma-glutamyglutamic acid. An enzyme that catalyzes the conversion of L-glutamic acid and 10-formyl-H4folic acid (also known as 10-formyl-H4pteroylglutamic acid) to 10-formyl-H4pteroyl-gamma-glutamylglutamic acid has been purified by 74-fold from extracts of Escherichia coli. ATP, Mg-2+, and a monovalent cation (K+ or NH-4, but not Na+) are required for the enzyme to function. Radioactive and bioautographic analyses revealed the formation of a single product. This product was identified as 10-formyl-H-4pteroyl-gamma-glutamylglutamic acid from its spectral characteristics, its ability to be used effectively as a growth faster for Lactobacillus casei 7469, and from radioactive analysis that indicated the incorporation into the product of 1 mol glutamate/mol of 10-formyl-H-4pteroylglutamic acid utilized. The enzyme functions optimally at pH 9.0-9.8 and at 50 degrees. Its molecular weight is estimated at 42,000-43,000. The Km values are 180 muM for L-glutamic acid and less than 2 muM for (-) 10-formyl-H-4pteroylglutamic acid. The only other naturally occurring folate compounds with significant activity as substrate are H-4pteroylglutamic acid and 5,10-methylene-H-4pteroylglutamic acid; however, these compounds are not used as effectively (K-m values are 10-12 mu-M) as 10-formyl-H-4pteroylglutamic acid."}
{"id": "PMID:1095055", "title": "The structure of the globin genes in chromatin.", "content": "The distribution of proteins in the neighborhood of the globin genes of duck reticulocyte chromatin has been studied. This chromatin is first shown to be an active template for transcription in vitro of globin messenger-like RNA. The chromatin is then treated with staphylococcal nuclease and the DNA fragments protected from nuclease attack (\"covered DNA\") are isolated. Alternatively, the chromatin is titrated with poly-D-lysine, and by successive treatment with Pronase and nuclease, the DNA regions accessible to polylysine are isolated (open \"DNA\"). In order to determine the distribution of globin gene sequences in open and covered DNA, these two fractions are annealed to globin cDNA (globin probe). It is found that while all globin gene sequences are represented in covered DNA, a specific portion of the globin gene is missing from open DNA, corresponding to about 20% of the gene length. It is concluded that specific regions of the globin genes of reticulocyte chromatin are partly covered by proteins in such a way as to render them in accessible to polylysine. In contrast, no difference is observed in the annealing properties of open and covered regions to globin probe using DNA isolated from erythrocyte chromatin, which is a poor templete in vitro for production of globin message. The annealing of open and covered DNA to each other has also been studied. It is found that open and covered DNA have identical sequence populations. Thus, in contrast to the special arrangement of proteins in the neighborhood of the globin gene, there does not appear to be any sequence-specific arrangement of the bulk of the chromatin proteins on chromatin DNA.", "contents": "The structure of the globin genes in chromatin. The distribution of proteins in the neighborhood of the globin genes of duck reticulocyte chromatin has been studied. This chromatin is first shown to be an active template for transcription in vitro of globin messenger-like RNA. The chromatin is then treated with staphylococcal nuclease and the DNA fragments protected from nuclease attack (\"covered DNA\") are isolated. Alternatively, the chromatin is titrated with poly-D-lysine, and by successive treatment with Pronase and nuclease, the DNA regions accessible to polylysine are isolated (open \"DNA\"). In order to determine the distribution of globin gene sequences in open and covered DNA, these two fractions are annealed to globin cDNA (globin probe). It is found that while all globin gene sequences are represented in covered DNA, a specific portion of the globin gene is missing from open DNA, corresponding to about 20% of the gene length. It is concluded that specific regions of the globin genes of reticulocyte chromatin are partly covered by proteins in such a way as to render them in accessible to polylysine. In contrast, no difference is observed in the annealing properties of open and covered regions to globin probe using DNA isolated from erythrocyte chromatin, which is a poor templete in vitro for production of globin message. The annealing of open and covered DNA to each other has also been studied. It is found that open and covered DNA have identical sequence populations. Thus, in contrast to the special arrangement of proteins in the neighborhood of the globin gene, there does not appear to be any sequence-specific arrangement of the bulk of the chromatin proteins on chromatin DNA."}
{"id": "PMID:1095056", "title": "Iodination of Escherichia coli lac repressor. Effect of tyrosine modification on repressor activity.", "content": "Treatment of Escherichia coli lac repressor with iodine (Kl3) at 4 degrees and pH 7.5 resulted in the rapid loss of repressor DNA binding activity. At a 30-fold molar excess of iodine to repressor, inactivation was complete within 15 sec. Inducer binding under the same conditions was only slightly affected. Iodinated repressor remained tetrameric, indicating that no gross structural alteration of the protein has taken place. Control experiments demonstrated that side products of the reaction did not contribute to the observed activity loss. Moreover, no restoration of binding activity was observed when iodinated repressor was assayed under a variety of assay conditions. Incubation of repressor with lac operator containing gamma plac DNA during the iodination reaction resulted in approximately 50% protection of binding activity. This protective effect was only partially operator specific, as gammaDNA lacking the operator binding site afforded roughly 25% protection under the same conditions. Incubation with either inducer and anti-inducer molecules during the iodination reaction did not protect repressor DNA binding activity. Iodination with K-131I-3 demonstrated that at complete inactivation virtually all of the bound iodine was recovered as iodotyrosine. A minor amount of cysteine oxidation to cysteinesulfonic acid was also detected. This oxidation encompassed no more than 30% of a single cysteine residue )tentatively identified as cysteine-107). Unstable intermediate oxidation products of cysteine did not appear to be involved in the loss of DNA binding activity. Modification of amino acids other than tyrosine and cysteine was not observed. Tryptic digestion of -131I-labeled repressor suggested that approximately 90% of the incorporated radioactivity was located in the repressor N-terminal tryptic peptide. Automated sequence analysis of iodinated repressor confirmed that at roughly 0.5 bound iodine atoms per repressor subunit (corresponding to approximately 5-10% activity loss) tyrosine residues 7, 12, and 17 were labeled in the ratios 1.0:0.5:0.8. Doubling the amount of bound iodine to 1.0 atom per subunit (corresponding to approximately 50-60% activity loss) did not significantly change the pattern of incorporation.", "contents": "Iodination of Escherichia coli lac repressor. Effect of tyrosine modification on repressor activity. Treatment of Escherichia coli lac repressor with iodine (Kl3) at 4 degrees and pH 7.5 resulted in the rapid loss of repressor DNA binding activity. At a 30-fold molar excess of iodine to repressor, inactivation was complete within 15 sec. Inducer binding under the same conditions was only slightly affected. Iodinated repressor remained tetrameric, indicating that no gross structural alteration of the protein has taken place. Control experiments demonstrated that side products of the reaction did not contribute to the observed activity loss. Moreover, no restoration of binding activity was observed when iodinated repressor was assayed under a variety of assay conditions. Incubation of repressor with lac operator containing gamma plac DNA during the iodination reaction resulted in approximately 50% protection of binding activity. This protective effect was only partially operator specific, as gammaDNA lacking the operator binding site afforded roughly 25% protection under the same conditions. Incubation with either inducer and anti-inducer molecules during the iodination reaction did not protect repressor DNA binding activity. Iodination with K-131I-3 demonstrated that at complete inactivation virtually all of the bound iodine was recovered as iodotyrosine. A minor amount of cysteine oxidation to cysteinesulfonic acid was also detected. This oxidation encompassed no more than 30% of a single cysteine residue )tentatively identified as cysteine-107). Unstable intermediate oxidation products of cysteine did not appear to be involved in the loss of DNA binding activity. Modification of amino acids other than tyrosine and cysteine was not observed. Tryptic digestion of -131I-labeled repressor suggested that approximately 90% of the incorporated radioactivity was located in the repressor N-terminal tryptic peptide. Automated sequence analysis of iodinated repressor confirmed that at roughly 0.5 bound iodine atoms per repressor subunit (corresponding to approximately 5-10% activity loss) tyrosine residues 7, 12, and 17 were labeled in the ratios 1.0:0.5:0.8. Doubling the amount of bound iodine to 1.0 atom per subunit (corresponding to approximately 50-60% activity loss) did not significantly change the pattern of incorporation."}
{"id": "PMID:1095057", "title": "A comparison of the fluorescence of the Y base of yeast tRNA-Phe in solution and in crystals.", "content": "The fluorescence properties of the Y base of yeast tRNA-Phe are known to be quite sensitive to the environment. The fluorescence lifetime of the Y base in yeast tRNA-Phe is identical in orthorhombic crystals and in the mother liquor from which these crystals are grown. It is 10% higher than the lifetime observed in dilute solutions of tRNA. This small change is a solvent effect due to isopropyl alcohol in the crystallization medium. Isopropyl alcohol does not change the accessibility of the chromophore of the Y base as measured by iodide quenching rates in solution. The accessibility in intact tRNA-Phe is much less than in a ribonuclease digest. Thus, within the limits of the sensitivity of the method, the Y chromophore occupies the same environment in solution and in the crystal and it must be at least partially buried.", "contents": "A comparison of the fluorescence of the Y base of yeast tRNA-Phe in solution and in crystals. The fluorescence properties of the Y base of yeast tRNA-Phe are known to be quite sensitive to the environment. The fluorescence lifetime of the Y base in yeast tRNA-Phe is identical in orthorhombic crystals and in the mother liquor from which these crystals are grown. It is 10% higher than the lifetime observed in dilute solutions of tRNA. This small change is a solvent effect due to isopropyl alcohol in the crystallization medium. Isopropyl alcohol does not change the accessibility of the chromophore of the Y base as measured by iodide quenching rates in solution. The accessibility in intact tRNA-Phe is much less than in a ribonuclease digest. Thus, within the limits of the sensitivity of the method, the Y chromophore occupies the same environment in solution and in the crystal and it must be at least partially buried."}
{"id": "PMID:1095058", "title": "Polyglycerophosphatide metabolism in Escherichia coli.", "content": "When Escherichia coli B cells were labelled with [14-C] glycerol and chased, there was a marked sparing of the phosphatidyl moiety compared to the nonacylated glycerol moiety of phosphatidylglycerol. When energy-depleted cells were restored to an energy-rich medium there resulted a conversion of 32-P-labelled cardiolipin to phosphatidylglycerol, a lack of phosphatidic acid accumulation and no loss in total polyglycerophosphatide counts. In cell-free extracts, phosphatidic acid produced from 32-P-labelled cardiolipin by the action of Escherichia coli phosphalipase D, was readily recycled to form poly-glycerophosphatide. In the presence of glycerol, such extracts displayed traansphosphatidylase activity by degrading cardiolipin to phosphatidyglycerol mainly. The results as a whole indicate that the enzyme synthesizing cardiolipin together with cardiolipin-hydrolyzing phospholipase D constitute a cycle which is normally involved in the turnover of polyglycerophosphatides in Escherichia coli.", "contents": "Polyglycerophosphatide metabolism in Escherichia coli. When Escherichia coli B cells were labelled with [14-C] glycerol and chased, there was a marked sparing of the phosphatidyl moiety compared to the nonacylated glycerol moiety of phosphatidylglycerol. When energy-depleted cells were restored to an energy-rich medium there resulted a conversion of 32-P-labelled cardiolipin to phosphatidylglycerol, a lack of phosphatidic acid accumulation and no loss in total polyglycerophosphatide counts. In cell-free extracts, phosphatidic acid produced from 32-P-labelled cardiolipin by the action of Escherichia coli phosphalipase D, was readily recycled to form poly-glycerophosphatide. In the presence of glycerol, such extracts displayed traansphosphatidylase activity by degrading cardiolipin to phosphatidyglycerol mainly. The results as a whole indicate that the enzyme synthesizing cardiolipin together with cardiolipin-hydrolyzing phospholipase D constitute a cycle which is normally involved in the turnover of polyglycerophosphatides in Escherichia coli."}
{"id": "PMID:1095059", "title": "Movement of calcium through artificial lipid membranes and the effects of ionophores.", "content": "The calcium efflux from multi-layered vesicles (liposomes) of different lipid composition has been studied. Liposomes composed of lipids extracted from cattle retinas are compared with liposomes which consist of phosphatidylcholine or a 1:1 phosphatidylcholine/phosphatidylserine mixture. The percentages of 45-Ca capture by these three types of liposomes are 10, 1 and 4% respectively; The efflux rates are 2.5-10- minus 6, 2-10- minus 6 and 4-10- minus 5 S- minus 1 respectively. The semilogarithmic efflux curves for phosphatidylcholine and phosphatidylcholine/phosphatidylserine liposomes are linear with time, but those for the retinal lipid liposomes are discontinuous. The activation energy for the calcium efflux from the latter liposomes is about 10.5 kcal/mol, both before and after the discontinuity. The ionophores X537A and A23187 enhance the calcium leakage from retinal lipid liposomes, the latter ionophore being much more effective than the former. At high concentrations both ionophores seem to transport calcium as a 1: 2 Ca-ionophore complex. At low ionophore concentrations, however, X537A appears to transport calcium as a 1:1 complex, but A23187 as a 2:1 complex.", "contents": "Movement of calcium through artificial lipid membranes and the effects of ionophores. The calcium efflux from multi-layered vesicles (liposomes) of different lipid composition has been studied. Liposomes composed of lipids extracted from cattle retinas are compared with liposomes which consist of phosphatidylcholine or a 1:1 phosphatidylcholine/phosphatidylserine mixture. The percentages of 45-Ca capture by these three types of liposomes are 10, 1 and 4% respectively; The efflux rates are 2.5-10- minus 6, 2-10- minus 6 and 4-10- minus 5 S- minus 1 respectively. The semilogarithmic efflux curves for phosphatidylcholine and phosphatidylcholine/phosphatidylserine liposomes are linear with time, but those for the retinal lipid liposomes are discontinuous. The activation energy for the calcium efflux from the latter liposomes is about 10.5 kcal/mol, both before and after the discontinuity. The ionophores X537A and A23187 enhance the calcium leakage from retinal lipid liposomes, the latter ionophore being much more effective than the former. At high concentrations both ionophores seem to transport calcium as a 1: 2 Ca-ionophore complex. At low ionophore concentrations, however, X537A appears to transport calcium as a 1:1 complex, but A23187 as a 2:1 complex."}
{"id": "PMID:1095060", "title": "Membrane reconstitution in chl-r mutants of Escherichia coli K 12. VIII. Purification and properties of the FA factor, the product of the chl B gene.", "content": "The isolation and purification of the product of the chl B gene of Escherichia coli K 12 from the chl A mutant have been attempted. The purified protein gives a single band in 10% sodium dodecylsulfate/polyacrylamide gel electrophoresis. The molecular weight is estimated to be 35 000. This protein, that we have named \"FA factor\", does not contain any lipid, has a strong tendency to lose its activity by polymerizing but can be kept in an active state when stored in buffer containing NaCl. The addition of purified FA protein to a soluble extract from the chl B mutant strain grown under anaerobiosis in the presence of nitrate initiates the \"complementation reaction\", i.e. the reconstitution of the nitrate reductase activity and the formation of particulate material similar to the native membrane with respect to the structure and enzymatic function. FA protein acts both on the rate of reconstitution and on the total amount of reconstituted enzyme. The complementation leads to the reconstitution of nonsedimentable nitrate reductase and to the formation of three types of particles of different buoyant densities (1.10, 1.18 and 1.23) the two lightest of which contain nitrate reductase. It is shown that FA factor is incorporated only into the particles of intermediate density. In vivo, this factor is located in the native membranes of chl A, chl C, chl D and wild-type strains, whatever the growth conditions, aerobiosis or anaerobiosis, and in the presence or absence of nitrate. Protein FA can be released from either of these membranes (native or reconstituted) by removing Mg-2+ or by subjecting Kaback's vesicles to mechanical treatments; in the case of 1.18-reconstituted particles and wild-type membranes, the release of FA protein does not exert any effect on the level of the nitrate reductase activity.", "contents": "Membrane reconstitution in chl-r mutants of Escherichia coli K 12. VIII. Purification and properties of the FA factor, the product of the chl B gene. The isolation and purification of the product of the chl B gene of Escherichia coli K 12 from the chl A mutant have been attempted. The purified protein gives a single band in 10% sodium dodecylsulfate/polyacrylamide gel electrophoresis. The molecular weight is estimated to be 35 000. This protein, that we have named \"FA factor\", does not contain any lipid, has a strong tendency to lose its activity by polymerizing but can be kept in an active state when stored in buffer containing NaCl. The addition of purified FA protein to a soluble extract from the chl B mutant strain grown under anaerobiosis in the presence of nitrate initiates the \"complementation reaction\", i.e. the reconstitution of the nitrate reductase activity and the formation of particulate material similar to the native membrane with respect to the structure and enzymatic function. FA protein acts both on the rate of reconstitution and on the total amount of reconstituted enzyme. The complementation leads to the reconstitution of nonsedimentable nitrate reductase and to the formation of three types of particles of different buoyant densities (1.10, 1.18 and 1.23) the two lightest of which contain nitrate reductase. It is shown that FA factor is incorporated only into the particles of intermediate density. In vivo, this factor is located in the native membranes of chl A, chl C, chl D and wild-type strains, whatever the growth conditions, aerobiosis or anaerobiosis, and in the presence or absence of nitrate. Protein FA can be released from either of these membranes (native or reconstituted) by removing Mg-2+ or by subjecting Kaback's vesicles to mechanical treatments; in the case of 1.18-reconstituted particles and wild-type membranes, the release of FA protein does not exert any effect on the level of the nitrate reductase activity."}
{"id": "PMID:1095061", "title": "Membrane reconstitution in chl-r mutants of Escherichia coli K 12. IX. Part played by phospholipids in the complementation process.", "content": "The supernatant extracts of the chl A and chl B mutants of Escherichia coli K 12, the phospholipids of which are labeled by growth in 32 P or [2- 3H]glycerol media, contain 20 times more radioactivity than the supernatant extract of the wild-type strain grown under the same conditions. We have observed that, after complementation, 80% of the radioactivity previously contained by Extracts A and B is incorporated into reconstituted particles. The chromatography of 3H-labeled Extract B on DEAE-cellulose and followed by gel filtration of radioactive fractions on Sephadex G-200 has shown that the phospholipids of Extract B are only bound to soluble proteins and not to fragments of membranes; it can be assumed that they have been solubilized in the form of a lipid-protein complex by cell breakage. When Extracts A and B are treated by phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) before being mixed together, an inhibition of the reconstitution of nitrate reductase activity which is proportional to the phospholipase C concentration and the length of treatment is observed. The analysis of lipids and phospholipids of particles (Peak I, Peak II and Peak III) formed during complementation and reconstituted nitrate reductase shows that their phospholipid contents (phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylserine) and especially that of Peak II (d equals 1.18) are closely related to that of native particles from the wild-type strain. These results allow one to propose a hypothesis explaining the mechanism involved in complementation.", "contents": "Membrane reconstitution in chl-r mutants of Escherichia coli K 12. IX. Part played by phospholipids in the complementation process. The supernatant extracts of the chl A and chl B mutants of Escherichia coli K 12, the phospholipids of which are labeled by growth in 32 P or [2- 3H]glycerol media, contain 20 times more radioactivity than the supernatant extract of the wild-type strain grown under the same conditions. We have observed that, after complementation, 80% of the radioactivity previously contained by Extracts A and B is incorporated into reconstituted particles. The chromatography of 3H-labeled Extract B on DEAE-cellulose and followed by gel filtration of radioactive fractions on Sephadex G-200 has shown that the phospholipids of Extract B are only bound to soluble proteins and not to fragments of membranes; it can be assumed that they have been solubilized in the form of a lipid-protein complex by cell breakage. When Extracts A and B are treated by phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) before being mixed together, an inhibition of the reconstitution of nitrate reductase activity which is proportional to the phospholipase C concentration and the length of treatment is observed. The analysis of lipids and phospholipids of particles (Peak I, Peak II and Peak III) formed during complementation and reconstituted nitrate reductase shows that their phospholipid contents (phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylserine) and especially that of Peak II (d equals 1.18) are closely related to that of native particles from the wild-type strain. These results allow one to propose a hypothesis explaining the mechanism involved in complementation."}
{"id": "PMID:1095062", "title": "Lipid phase transitions in cytoplasmic and outer membranes of Escherichia coli.", "content": "The cytoplasmic and outer membranes containing either trans-delta-9-octadecenoate, trans-delta-9-hexadecenoate or cis-delta-9-octadecenoate as predominant unsaturated fatty acid residues in the phospholipids were prepared from a fatty acid auxotroph, Escherichia coli strain K1062. Order-disorder transitions of the phospholipids were revealed in both fractions of the cell envelope by fluorescent probing or wide angle X-ray diffraction. The mid-transition temperatures, Tt, and the range of the transition, delta-T, are similar in the outer and cytoplasmic membrane. Relative to the corresponding extracted lipids, 60-80% of the hydrocarbon chains take part in the transition in the cytoplasmic membrane whereas in the outer membrane only 25-40% of the chains become ordered. The results suggest that in the outer membrane part of the lipids form fluid domains in the form of mono- and/or bilayers.", "contents": "Lipid phase transitions in cytoplasmic and outer membranes of Escherichia coli. The cytoplasmic and outer membranes containing either trans-delta-9-octadecenoate, trans-delta-9-hexadecenoate or cis-delta-9-octadecenoate as predominant unsaturated fatty acid residues in the phospholipids were prepared from a fatty acid auxotroph, Escherichia coli strain K1062. Order-disorder transitions of the phospholipids were revealed in both fractions of the cell envelope by fluorescent probing or wide angle X-ray diffraction. The mid-transition temperatures, Tt, and the range of the transition, delta-T, are similar in the outer and cytoplasmic membrane. Relative to the corresponding extracted lipids, 60-80% of the hydrocarbon chains take part in the transition in the cytoplasmic membrane whereas in the outer membrane only 25-40% of the chains become ordered. The results suggest that in the outer membrane part of the lipids form fluid domains in the form of mono- and/or bilayers."}
{"id": "PMID:1095063", "title": "Comparison of the myoglobin of the zebra (Equus burchelli) with that of the horse (Equus caballus).", "content": "The tryptic and peptic peptides from the myoglobin of the zebra (Equus burchelli) have been compared with those obtained from the myoglobin of the horse (Equus caballus). No differences in the myoglobin were found between these two species.", "contents": "Comparison of the myoglobin of the zebra (Equus burchelli) with that of the horse (Equus caballus). The tryptic and peptic peptides from the myoglobin of the zebra (Equus burchelli) have been compared with those obtained from the myoglobin of the horse (Equus caballus). No differences in the myoglobin were found between these two species."}
{"id": "PMID:1095064", "title": "The myoglobin of primates. VII. Perodicticus potto edwarsi (potto).", "content": "The primary structure of the myoglobin of the prosimian Lorisidae Perodictius potto edwarsi (potto) was studied. Tryptic, chymotryptic, peptic, subtilisin and thermolysin peptides were aligned against the sequence of human myoglobin. Sixteen differences were found which were confirmed by sequential analysis. On comparison of the West African potto with two other prosimian myoglobins known so far, there were 12 differences between the potto and the galago (East African) and 18 differences between the potto and the sportive lemur (Madagascar).", "contents": "The myoglobin of primates. VII. Perodicticus potto edwarsi (potto). The primary structure of the myoglobin of the prosimian Lorisidae Perodictius potto edwarsi (potto) was studied. Tryptic, chymotryptic, peptic, subtilisin and thermolysin peptides were aligned against the sequence of human myoglobin. Sixteen differences were found which were confirmed by sequential analysis. On comparison of the West African potto with two other prosimian myoglobins known so far, there were 12 differences between the potto and the galago (East African) and 18 differences between the potto and the sportive lemur (Madagascar)."}
{"id": "PMID:1095065", "title": "High affinity phlorizin receptor sites and their relation to the glucose transport mechanism in the proximal tubule of dog kidney.", "content": "A set of high affinity phlorizin receptors in a brush border membrane preparation from dog kidney cortex is described. The dissociation constant, Kd is approximately equal to 0.3 muM (20 mM Tris-HCl, 150 mM Na-+, 5mM EDTA pH 7.45, 37 degree C). The number of receptor sites is approximately equal to 12-10- minus 12 mol/mg membrane protein. Preincubation with sugar substrates shows that the high affinity phlorizin binding is completely abolished by D-glucose (100 mM), 3-deoxy-30fluoro-D-glucose (125 mM) and alpha-methyl-D-glucopyranoside (125 mM), while 40-50% inhibition is observed with glucose concentrations as low as 5 mM. D-Galactose adn beta-methyl-D-galactopyranoside inhibit 20-40% at 125 mM while 2-deoxy-D-glucose and 2-deoxy-D-galactose inhibit minimally (approximately equal to 25%) at the same concentration. L-glucose, D-mannose, D-xylose, myoinositol, D-fructose and 3-O-methyl-D-glucose do not inhibit significantly in concentrations up to 600 mM. Unlabelled phlorizin (1 muM) and D-glucose (125 mM) completely wash off bound [3-H] phlorizin from the high affinity site. In contrast, phloretin (100 muM) is only about 50% as effective in displacing bound [3-H] phlorizin. Binding decreases with decreasing sodium concentration and is abolished by N-ethylmaleimide (7 mM). No inhibition is observed with ouabain (0.125 mM), cytochalasin B (0.1- 42 muM) and concanavalin A (10-10 000 mug/ml). The specificity of inhibition of phlorizin binding in vivo to the luminal membrane of the proximal tubule in dog kidney has also been investigated. Alpha-methyl-D-glucopyranoside completely washes off bound [3-H] phlorizin. D-galactose is only about 10% as effective at equivalent doses. There is no observable wash off of bound [3-H] phlorizin with D-fructose, myoinositol, D-mannose or 2-deoxy-D-glucose. The relative affinity of monosaccharides for the glucose transport receptor at the brush border was investigated in vivo using the multiple indicator dilution technique to determine their fractional reabsorption under identical conditions of phlorizin blockade. The relative affinities are in the order D-glucose approximately equal to alpha-methyl-D-glucopyranoside greater than D-galactose greater than 2-deoxy-D-glucose greater than D-fructose approximately equal to myoinositol. It is concluded (i) that phlorizin receptors on the brush border of the proximal tubule in vivo are identical to the high affinity phlorizin binding sites in the brush border membrane fraction in vitro and (ii) that these phlorizin receptor sites are either in close proximity, or identical, to the glucose transport receptor.", "contents": "High affinity phlorizin receptor sites and their relation to the glucose transport mechanism in the proximal tubule of dog kidney. A set of high affinity phlorizin receptors in a brush border membrane preparation from dog kidney cortex is described. The dissociation constant, Kd is approximately equal to 0.3 muM (20 mM Tris-HCl, 150 mM Na-+, 5mM EDTA pH 7.45, 37 degree C). The number of receptor sites is approximately equal to 12-10- minus 12 mol/mg membrane protein. Preincubation with sugar substrates shows that the high affinity phlorizin binding is completely abolished by D-glucose (100 mM), 3-deoxy-30fluoro-D-glucose (125 mM) and alpha-methyl-D-glucopyranoside (125 mM), while 40-50% inhibition is observed with glucose concentrations as low as 5 mM. D-Galactose adn beta-methyl-D-galactopyranoside inhibit 20-40% at 125 mM while 2-deoxy-D-glucose and 2-deoxy-D-galactose inhibit minimally (approximately equal to 25%) at the same concentration. L-glucose, D-mannose, D-xylose, myoinositol, D-fructose and 3-O-methyl-D-glucose do not inhibit significantly in concentrations up to 600 mM. Unlabelled phlorizin (1 muM) and D-glucose (125 mM) completely wash off bound [3-H] phlorizin from the high affinity site. In contrast, phloretin (100 muM) is only about 50% as effective in displacing bound [3-H] phlorizin. Binding decreases with decreasing sodium concentration and is abolished by N-ethylmaleimide (7 mM). No inhibition is observed with ouabain (0.125 mM), cytochalasin B (0.1- 42 muM) and concanavalin A (10-10 000 mug/ml). The specificity of inhibition of phlorizin binding in vivo to the luminal membrane of the proximal tubule in dog kidney has also been investigated. Alpha-methyl-D-glucopyranoside completely washes off bound [3-H] phlorizin. D-galactose is only about 10% as effective at equivalent doses. There is no observable wash off of bound [3-H] phlorizin with D-fructose, myoinositol, D-mannose or 2-deoxy-D-glucose. The relative affinity of monosaccharides for the glucose transport receptor at the brush border was investigated in vivo using the multiple indicator dilution technique to determine their fractional reabsorption under identical conditions of phlorizin blockade. The relative affinities are in the order D-glucose approximately equal to alpha-methyl-D-glucopyranoside greater than D-galactose greater than 2-deoxy-D-glucose greater than D-fructose approximately equal to myoinositol. It is concluded (i) that phlorizin receptors on the brush border of the proximal tubule in vivo are identical to the high affinity phlorizin binding sites in the brush border membrane fraction in vitro and (ii) that these phlorizin receptor sites are either in close proximity, or identical, to the glucose transport receptor."}
{"id": "PMID:1095066", "title": "Phospholipase activity plays no role in the action of colicin K.", "content": "1. A mutant lacking both detergent-resistant and detergent-sensitive phospholipase A activities is fully sensitive to colicin  K. 2. In the absence of cellular phospholipases A, colicin K does not promote hydrolysis of phosphatidylethanolamine. 3. Cells of the colicin-treated mutant lacking lysophosphatidylethanolamine are as abnormally permeable to Co-2+ as the wind type is. 4. Increased levels of lysophosphatidylethanolamine in colicin-treated cells are not necessary for the increased sensitivity to sodium dodecylsulfate.", "contents": "Phospholipase activity plays no role in the action of colicin K. 1. A mutant lacking both detergent-resistant and detergent-sensitive phospholipase A activities is fully sensitive to colicin  K. 2. In the absence of cellular phospholipases A, colicin K does not promote hydrolysis of phosphatidylethanolamine. 3. Cells of the colicin-treated mutant lacking lysophosphatidylethanolamine are as abnormally permeable to Co-2+ as the wind type is. 4. Increased levels of lysophosphatidylethanolamine in colicin-treated cells are not necessary for the increased sensitivity to sodium dodecylsulfate."}
{"id": "PMID:1095067", "title": "NMR investigation of the effect of selective modifications in the anticodon loop on the conformation of yeast transfer RNA-Phe.", "content": "Changes in the 300 MHz proton NMR spectrum of yeast tRNA-Phe induced by the removal of the Y-base from the anticodon loop are reversed when the dye proflavine is incorporated in its place. These observations correlate with our earlier interpretation of the NMR data that removal of the Y-base causes a change in the conformation of the anticodon stem. Such changes in stem conformation may in part be responsible for the differences in the biochemical properties of tRNA-Phe, tRNA-PhePF and tRNA-Phe-Y.", "contents": "NMR investigation of the effect of selective modifications in the anticodon loop on the conformation of yeast transfer RNA-Phe. Changes in the 300 MHz proton NMR spectrum of yeast tRNA-Phe induced by the removal of the Y-base from the anticodon loop are reversed when the dye proflavine is incorporated in its place. These observations correlate with our earlier interpretation of the NMR data that removal of the Y-base causes a change in the conformation of the anticodon stem. Such changes in stem conformation may in part be responsible for the differences in the biochemical properties of tRNA-Phe, tRNA-PhePF and tRNA-Phe-Y."}
{"id": "PMID:1095068", "title": "The isolation of large polysomes in high yield from unfractionated tissue homogenates.", "content": "It was found that if large quantities of both exogenous RNA and Mg-2+ were present during gentle tissue homogenization, the subsequent addition of deoxycholate to the whole homogenate produced a viscous mass from which polysomes could be isolated in large yields. These polysomes were substantially less degraded than those isolated by previous methods. In the case of rat liver, 15 ribosomes per mRNA was the species present in highest concentration. The parameters of this method were investigated and optimized. About 80 percent of the rRNA in the homogenates was recovered in the polysomes. Omission of deoxycholate permitted the isolation of less-degraded free polysomes as well. In the liver of fed rats these represented one-fourth of the total polysomes, in good agreement with results obtained by an independent approach. Using the method to isolate polysomes from the liver of starving rats, it was found that only about one percent of the large amount of monomers and dimers present resulted from polysome breakdown during isolation. It was further shown that random RNAase hydrolysis of polysomes could not produce the patterns of liver polysomes seen during starvation. Polysomes isolated by this procedure were quite stable in solution and were very active in cell-free protein synthesis. Application of this method without adaptation to eight other tissues also permitted the isolation of large polysomes in high yields.", "contents": "The isolation of large polysomes in high yield from unfractionated tissue homogenates. It was found that if large quantities of both exogenous RNA and Mg-2+ were present during gentle tissue homogenization, the subsequent addition of deoxycholate to the whole homogenate produced a viscous mass from which polysomes could be isolated in large yields. These polysomes were substantially less degraded than those isolated by previous methods. In the case of rat liver, 15 ribosomes per mRNA was the species present in highest concentration. The parameters of this method were investigated and optimized. About 80 percent of the rRNA in the homogenates was recovered in the polysomes. Omission of deoxycholate permitted the isolation of less-degraded free polysomes as well. In the liver of fed rats these represented one-fourth of the total polysomes, in good agreement with results obtained by an independent approach. Using the method to isolate polysomes from the liver of starving rats, it was found that only about one percent of the large amount of monomers and dimers present resulted from polysome breakdown during isolation. It was further shown that random RNAase hydrolysis of polysomes could not produce the patterns of liver polysomes seen during starvation. Polysomes isolated by this procedure were quite stable in solution and were very active in cell-free protein synthesis. Application of this method without adaptation to eight other tissues also permitted the isolation of large polysomes in high yields."}
{"id": "PMID:1095070", "title": "Regulation of RNA synthesis in Escherichia coli during a shift-up transition.", "content": "These experiments investigate two aspects of RNA synthesis in Escherichia coli ML30 during the transition from a relatively slow rate of growth to a more rapid one: (1) the number of growing RNA molecules per cell, and (2) the average time required for addition of a nucleotide onto a growing RNA chain. Cells were grown at 30 degrees C in a glucose-minimal salts medium and shifted-up by the addition of Casamino acids. Measurements were made of the rates of incorporation over short intervals (e.g. 5,8,12, and 16 s) of [3-H]guanine into the internal and 3'-terminal nucleotides of RNA. After correction for the specific activities of the intracellular GTP pools, and for the rate of [3-H]guanine accumulation at the 3'-terminus of non-growing RNA, the rates of chain elongation were calculated. It was found that cells growing at a rate of 0.9 generations/h contain approx. 4800 RNA molecules, growing at a rate of 28 nucleotides/s per chain. Cells growing exponentially at the postshift-up rate (1.2 generations/h) contain 7000 RNA molecules per unit equivalent cell mass, which are growing at a rate of 32 nucleotides/s per molecule. Three min after shift-up, cells contain the same number or slightly fewer (10%) growing RNA molecules than cells prior to shift-up, 4300, and these are being elongated at a rate of about 32 nucleotides per s. The results are consistent with the view that in the range of growth rates studied, the total rate of RNA synthesis is regulated through a limitation in the number of functioning RNA polymerase molecules, each working at a relatively constant, presumably maximal, average rate.", "contents": "Regulation of RNA synthesis in Escherichia coli during a shift-up transition. These experiments investigate two aspects of RNA synthesis in Escherichia coli ML30 during the transition from a relatively slow rate of growth to a more rapid one: (1) the number of growing RNA molecules per cell, and (2) the average time required for addition of a nucleotide onto a growing RNA chain. Cells were grown at 30 degrees C in a glucose-minimal salts medium and shifted-up by the addition of Casamino acids. Measurements were made of the rates of incorporation over short intervals (e.g. 5,8,12, and 16 s) of [3-H]guanine into the internal and 3'-terminal nucleotides of RNA. After correction for the specific activities of the intracellular GTP pools, and for the rate of [3-H]guanine accumulation at the 3'-terminus of non-growing RNA, the rates of chain elongation were calculated. It was found that cells growing at a rate of 0.9 generations/h contain approx. 4800 RNA molecules, growing at a rate of 28 nucleotides/s per chain. Cells growing exponentially at the postshift-up rate (1.2 generations/h) contain 7000 RNA molecules per unit equivalent cell mass, which are growing at a rate of 32 nucleotides/s per molecule. Three min after shift-up, cells contain the same number or slightly fewer (10%) growing RNA molecules than cells prior to shift-up, 4300, and these are being elongated at a rate of about 32 nucleotides per s. The results are consistent with the view that in the range of growth rates studied, the total rate of RNA synthesis is regulated through a limitation in the number of functioning RNA polymerase molecules, each working at a relatively constant, presumably maximal, average rate."}
{"id": "PMID:1095071", "title": "Ribosomal and non-ribosomal RNA synthesis in vitro.", "content": "The synthesis of total and ribosomal RNA using nucleoids of Escherichia coli as template was measured; of the total RNA synthesized by endogenous RNA polymerase which only completes chains, and added RNA polymerase which initiates new chains, 50-70 and 3-5%, repectively, was rRNA. Total RNA synthesis by added enzyme, however, was 10-20 times higher than endogenous RNA synthesis; thus rRNA was synthesized at the same rate by the endogenous and the added enzyme. We conclude that the percentage rRNA in vitro is no measure of the rate of rRNA synthesis. Furthermore, it follows that the added enzyme, like the endogenous one, is packed at the physical limit on the ribosomal cistrons. Consequently, initiation of ribosomal cistrons by added enzyme was at or near the maximal rate possible for this system in which the elongation rate is 10-20% of that in vitro. When RNA synthesis was assayed at various ratios of RNA polymerase to phenol-extracted DNA, the amount of rRNA made per DNA, which is a measure of the frequency of transcription of ribosomal cistrons, varied. The ratio of rRNA synthesis relative to total RNA synthesis also varied, but in a different way, again leading to the conclusion that this ratio, as determined in vitro, does not reflect the efficiency of transcription of the ribosomal cistrons.", "contents": "Ribosomal and non-ribosomal RNA synthesis in vitro. The synthesis of total and ribosomal RNA using nucleoids of Escherichia coli as template was measured; of the total RNA synthesized by endogenous RNA polymerase which only completes chains, and added RNA polymerase which initiates new chains, 50-70 and 3-5%, repectively, was rRNA. Total RNA synthesis by added enzyme, however, was 10-20 times higher than endogenous RNA synthesis; thus rRNA was synthesized at the same rate by the endogenous and the added enzyme. We conclude that the percentage rRNA in vitro is no measure of the rate of rRNA synthesis. Furthermore, it follows that the added enzyme, like the endogenous one, is packed at the physical limit on the ribosomal cistrons. Consequently, initiation of ribosomal cistrons by added enzyme was at or near the maximal rate possible for this system in which the elongation rate is 10-20% of that in vitro. When RNA synthesis was assayed at various ratios of RNA polymerase to phenol-extracted DNA, the amount of rRNA made per DNA, which is a measure of the frequency of transcription of ribosomal cistrons, varied. The ratio of rRNA synthesis relative to total RNA synthesis also varied, but in a different way, again leading to the conclusion that this ratio, as determined in vitro, does not reflect the efficiency of transcription of the ribosomal cistrons."}
{"id": "PMID:1095072", "title": "Quinone induced stringent control. Accumulation of ppGpp and inhibition of RNA synthesis in stringent Escherichia coli by 5,8-dioxo-6-amino-7-chloroquinoline.", "content": "The mode of action of a synthetic cytostatic quinone was studied in Escherichia coli. 1. At concentrations of 1.5-6 mug/ml, 5,8-dioxo-6-amino-7-chloroquinoline rapidly inhibits growth and protein synthesis in E. coli. The synthesis of RNA is immediately affected in E. coli rel+ whereas in E. coli rel- the accumulation of RNA can proceed on addition of the quinone. This indicates that the inhibition of RNA synthesis in the stringent strain is a consequence of the regulatory phenomenon governed by the rel gene. 2. Chloramphenicol, known to abolish the stringent control mechanism, added simultaneously with the quinone allows the accumulation of RNA to proceed in the stringent strain. 3. Guanosine tetraphosphate accumulates in quinone-treated E. coli rel+ but not in the relaxed mutant strain. 4. Addition of amino acids reverses all inhibitory effects observed in quinone treated stringent and relaxed cells. 5. It is concluded that the bacteriostatic effect of 5,8-dioxo-6-amino-7-chloroquinoline on E. coli is caused by an apparent intracellular amino acid starvation.", "contents": "Quinone induced stringent control. Accumulation of ppGpp and inhibition of RNA synthesis in stringent Escherichia coli by 5,8-dioxo-6-amino-7-chloroquinoline. The mode of action of a synthetic cytostatic quinone was studied in Escherichia coli. 1. At concentrations of 1.5-6 mug/ml, 5,8-dioxo-6-amino-7-chloroquinoline rapidly inhibits growth and protein synthesis in E. coli. The synthesis of RNA is immediately affected in E. coli rel+ whereas in E. coli rel- the accumulation of RNA can proceed on addition of the quinone. This indicates that the inhibition of RNA synthesis in the stringent strain is a consequence of the regulatory phenomenon governed by the rel gene. 2. Chloramphenicol, known to abolish the stringent control mechanism, added simultaneously with the quinone allows the accumulation of RNA to proceed in the stringent strain. 3. Guanosine tetraphosphate accumulates in quinone-treated E. coli rel+ but not in the relaxed mutant strain. 4. Addition of amino acids reverses all inhibitory effects observed in quinone treated stringent and relaxed cells. 5. It is concluded that the bacteriostatic effect of 5,8-dioxo-6-amino-7-chloroquinoline on E. coli is caused by an apparent intracellular amino acid starvation."}
{"id": "PMID:1095073", "title": "Affinity-labelling of Escherichia coli ribosomes by a derivative of phenylalanyl-tRNA. A critical test for the specificity.", "content": "Under nonenzymatic binding conditions p-nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p-nitrophenoxycarbonyl-phenylalanyl-tRNA.", "contents": "Affinity-labelling of Escherichia coli ribosomes by a derivative of phenylalanyl-tRNA. A critical test for the specificity. Under nonenzymatic binding conditions p-nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p-nitrophenoxycarbonyl-phenylalanyl-tRNA."}
{"id": "PMID:1095074", "title": "Some effects of calcium and magnesium ions on the activity of bovine pancreatic deoxyribonuclease A.", "content": "Bovine pancreatic deoxyribonuclease requires divalent metal cations for hydrolysis of DNA. The effects of calcium and magnesium, alone and combined, on the rate and kinetics of the reaction were examined. Divalent metal salts of DNA were used as substrates. The ratio of either Ca-2+ or Mg-2+ to DNA-P in these salts was 1:2. The Mg-2+ salt of DNA was found to have sufficient Mg-2+ for optimal DNAase activity. Addition of MgCl-2 to a large excess of Mg-2+ over DNA-P had no effect on the rate. Km for the hydrolysis of Mg-2+-DNA was 1.76 mM. Km for the hydrolysis of Ca-2+-DNA was too low to measure by our  methods of assay, indicating a high affinity of enzyme for substrate. The rate of hydrolysis of Ca-2+-DNA, however, is slow compared to that of Mg-2+-DNA. By mixing the Ca-2+ and Mg-2+ salts of DNA, a synergistic effect on the activity of DNAase was observed. On the basis of kinetic studies the synergistic effect is attributed to an increased affinity of DNAase for DNA (Km equals 0.34 mM for the mixed Ca-2+, Mg-2+ salt of DNA). A 2-fold increase in DNAase activity was observed when DNAase was incubated in CaCl-2 before assay. This 2-fold stimulation was not related to the synergistic effect. DNAase I is inactive against the sodium salt of DNA. In experiments with mixed Na+ and Mg-2+ salts of DNA, Na+-DNA was found to be a competitive inhibitor of the action of DNAase against Mg-2+-DNA.", "contents": "Some effects of calcium and magnesium ions on the activity of bovine pancreatic deoxyribonuclease A. Bovine pancreatic deoxyribonuclease requires divalent metal cations for hydrolysis of DNA. The effects of calcium and magnesium, alone and combined, on the rate and kinetics of the reaction were examined. Divalent metal salts of DNA were used as substrates. The ratio of either Ca-2+ or Mg-2+ to DNA-P in these salts was 1:2. The Mg-2+ salt of DNA was found to have sufficient Mg-2+ for optimal DNAase activity. Addition of MgCl-2 to a large excess of Mg-2+ over DNA-P had no effect on the rate. Km for the hydrolysis of Mg-2+-DNA was 1.76 mM. Km for the hydrolysis of Ca-2+-DNA was too low to measure by our  methods of assay, indicating a high affinity of enzyme for substrate. The rate of hydrolysis of Ca-2+-DNA, however, is slow compared to that of Mg-2+-DNA. By mixing the Ca-2+ and Mg-2+ salts of DNA, a synergistic effect on the activity of DNAase was observed. On the basis of kinetic studies the synergistic effect is attributed to an increased affinity of DNAase for DNA (Km equals 0.34 mM for the mixed Ca-2+, Mg-2+ salt of DNA). A 2-fold increase in DNAase activity was observed when DNAase was incubated in CaCl-2 before assay. This 2-fold stimulation was not related to the synergistic effect. DNAase I is inactive against the sodium salt of DNA. In experiments with mixed Na+ and Mg-2+ salts of DNA, Na+-DNA was found to be a competitive inhibitor of the action of DNAase against Mg-2+-DNA."}
{"id": "PMID:1095075", "title": "[Ribosome stability of Escherichia coli cells in amino acid starvation].", "content": "The dissociation of ribosomes from two isogenic pairs of Escherichia coli strains was studied during exponential growth, under amino acid starvation and subsequent chloramphenicol treatment. There were no significant differences in Mg2+-dependent dissociation of ribosomes from exponentially growing rel+ and rel- minus strains. The differences in dissociation of the ribosomes from rel+ and rel- minus cells were observed only upon amino acid starvation of these cultures. The dissociation of ribosomes from starved rel+ cells was more complete. After chloramphenicol treatment isolated ribosomes were more resistant to dissociation into subunits. Alterations of dissociation of the ribosomes in vitro correlated both with the amount of polysomes and the level of RNA synthesis in cells. It is proposed that rRNA synthesis in bacteria depends on the ratio of programmed and deprogrammed ribosomes.", "contents": "[Ribosome stability of Escherichia coli cells in amino acid starvation]. The dissociation of ribosomes from two isogenic pairs of Escherichia coli strains was studied during exponential growth, under amino acid starvation and subsequent chloramphenicol treatment. There were no significant differences in Mg2+-dependent dissociation of ribosomes from exponentially growing rel+ and rel- minus strains. The differences in dissociation of the ribosomes from rel+ and rel- minus cells were observed only upon amino acid starvation of these cultures. The dissociation of ribosomes from starved rel+ cells was more complete. After chloramphenicol treatment isolated ribosomes were more resistant to dissociation into subunits. Alterations of dissociation of the ribosomes in vitro correlated both with the amount of polysomes and the level of RNA synthesis in cells. It is proposed that rRNA synthesis in bacteria depends on the ratio of programmed and deprogrammed ribosomes."}
{"id": "PMID:1095077", "title": "[2 phosphotransferase systems that control the second stage of phosphoenolpyruvate-dependent glucose phosphorylation in E. coli].", "content": "Analysis of E. coli W mutants defective in glucose transport suggests an existence of two enzyme systems which carry out the second step of phosphoenolpyruvate (PEP)-dependent glucose phosphorylation, thus controlling the glucose transport into E. coli cells. One system (PTS-glu-alpha) controls phosphorylation and transport of both glucose and alpha-methylglucoside, the other system (PTS-glu-beta) controls phosphorylation and transport of glucose only. PTS-glu-alpha system is damaged in the mutants studied, but PTS-glu-beta system is intact. On account of this fact the mutants do not uptake 14-C-alpha-methylglucoside and their extracts are practically incapable of its phosphorylation, phosphoenolpyruvate being used as phosphate donor. At the same time the mutants are capable of 14-C-glucose uptake and PEP-dependent 14-C-glucose phosphorylation. In one of the strain, having the intact PTS-glu-alpha system, the uptake of glucose and alpha-methylglucoside was stimulated by the addiction of glucose in the cultural medium. No such effect was observed in bacteria with the disturbed PTS-glu-alpha system and the intact PTS-glu-beta system. Probably, the PTS-glu-alpha system can be inducible, in contrast with the PTS-glu-beta system. The damage of the PTS-glu-alpha system results in resistance of bacteria to glucose-induced inhibition of the enzyme synthesis.", "contents": "[2 phosphotransferase systems that control the second stage of phosphoenolpyruvate-dependent glucose phosphorylation in E. coli]. Analysis of E. coli W mutants defective in glucose transport suggests an existence of two enzyme systems which carry out the second step of phosphoenolpyruvate (PEP)-dependent glucose phosphorylation, thus controlling the glucose transport into E. coli cells. One system (PTS-glu-alpha) controls phosphorylation and transport of both glucose and alpha-methylglucoside, the other system (PTS-glu-beta) controls phosphorylation and transport of glucose only. PTS-glu-alpha system is damaged in the mutants studied, but PTS-glu-beta system is intact. On account of this fact the mutants do not uptake 14-C-alpha-methylglucoside and their extracts are practically incapable of its phosphorylation, phosphoenolpyruvate being used as phosphate donor. At the same time the mutants are capable of 14-C-glucose uptake and PEP-dependent 14-C-glucose phosphorylation. In one of the strain, having the intact PTS-glu-alpha system, the uptake of glucose and alpha-methylglucoside was stimulated by the addiction of glucose in the cultural medium. No such effect was observed in bacteria with the disturbed PTS-glu-alpha system and the intact PTS-glu-beta system. Probably, the PTS-glu-alpha system can be inducible, in contrast with the PTS-glu-beta system. The damage of the PTS-glu-alpha system results in resistance of bacteria to glucose-induced inhibition of the enzyme synthesis."}
{"id": "PMID:1095081", "title": "Comparative symptomatological and evoked potential studies with d-amphetamine, thioridazine, and placebo in hyperkinetic children.", "content": "In a double-blind study, 62 hyperkinetic children were randomly assigned to 8 weeks of treatment with either placebo, thioridazine, or d-amphetamine. The overall clinical symptomatology improved with all three substances, although d-amphetamine was significantly superior to placebo and thioridazine. Out of eight symptom clusters rated by the parents, two improved significantly with placebo, one with thioridazine, and six with d-amphetamine. The d-amphetamine was superior to placebo in reducing muscular tension and superior to thioridazine in decreasing hyperactive-impulsive behavior, psychosomatic problems, and muscular tension. Out of four teachers' symptom clusters, inattentive-passive behavior was significantly improved by thioridazine (which was also superior to placebo), while hyperactivity was reduced by d-amphetamine. Quantitative evaluation of visual evoked potentials (VEPs) revealed an increase in latencies and decrease in amplitudes during thioridazine treatment. Paradoxically, d-amphetamine also increased latencies, while tending to augment amplitudes. Regression and correlation analysis of clinical symptomatology with VEP variables showed that the shorter the pretreatment latencies and the higher the amplitudes, the more disturbed was the child. Short latencies and small amplitudes in the pretreatment period were predictors of good therapeutic outcome with subsequent thioridazine treatment, while short latencies and high amplitudes were indicative of such with d-amphetamine treatment. During therapy, the greater the drug-induced augmentation of latencies, the greater the clinical improvement. Finally, VEP differences between therapy-responsive and -resistant patients were explored and discussed.", "contents": "Comparative symptomatological and evoked potential studies with d-amphetamine, thioridazine, and placebo in hyperkinetic children. In a double-blind study, 62 hyperkinetic children were randomly assigned to 8 weeks of treatment with either placebo, thioridazine, or d-amphetamine. The overall clinical symptomatology improved with all three substances, although d-amphetamine was significantly superior to placebo and thioridazine. Out of eight symptom clusters rated by the parents, two improved significantly with placebo, one with thioridazine, and six with d-amphetamine. The d-amphetamine was superior to placebo in reducing muscular tension and superior to thioridazine in decreasing hyperactive-impulsive behavior, psychosomatic problems, and muscular tension. Out of four teachers' symptom clusters, inattentive-passive behavior was significantly improved by thioridazine (which was also superior to placebo), while hyperactivity was reduced by d-amphetamine. Quantitative evaluation of visual evoked potentials (VEPs) revealed an increase in latencies and decrease in amplitudes during thioridazine treatment. Paradoxically, d-amphetamine also increased latencies, while tending to augment amplitudes. Regression and correlation analysis of clinical symptomatology with VEP variables showed that the shorter the pretreatment latencies and the higher the amplitudes, the more disturbed was the child. Short latencies and small amplitudes in the pretreatment period were predictors of good therapeutic outcome with subsequent thioridazine treatment, while short latencies and high amplitudes were indicative of such with d-amphetamine treatment. During therapy, the greater the drug-induced augmentation of latencies, the greater the clinical improvement. Finally, VEP differences between therapy-responsive and -resistant patients were explored and discussed."}
{"id": "PMID:1095082", "title": "Phenethylamine in normal urine: failure to verify high values.", "content": "The phenethylamine (PEA) level in daytime urine samples of 18 normal subjects was measured by an isotope dilution technique. Purification of PEA from other urinary substance, after the addition of 1-14C-PEA to urine samples, was achieved by the sequence: thin-layer chromatography (TLC), derivatization, TLC, and finally, gas chromatography (electron capture). The estimated mean daily output of PEA was 10.3 mug/day. A circadian rhythm was not evident on the basis of analyses of 24-hr urine collections from four normal subjects. Three acute schizophrenics had levels within the normal means.", "contents": "Phenethylamine in normal urine: failure to verify high values. The phenethylamine (PEA) level in daytime urine samples of 18 normal subjects was measured by an isotope dilution technique. Purification of PEA from other urinary substance, after the addition of 1-14C-PEA to urine samples, was achieved by the sequence: thin-layer chromatography (TLC), derivatization, TLC, and finally, gas chromatography (electron capture). The estimated mean daily output of PEA was 10.3 mug/day. A circadian rhythm was not evident on the basis of analyses of 24-hr urine collections from four normal subjects. Three acute schizophrenics had levels within the normal means."}
{"id": "PMID:1095078", "title": "[Purification of microbial asparaginases by the aid of affinity chromatography].", "content": "Asparaginases from Escherichia coli and Erwinia caratovora were isolated and purified by column chromatography with a specific sorbent (sepharose, covalently bound to N-alpha-(6-aminohexyl)-D-asparagine). Homogenous asparaginase from E. coli was isolated by one-step procedure, while the enzyme from Er. carotovara was 50-60-fold purified. Asparaginase from Mycobacterium n. sp. was found not to bind with the specific sorbent.", "contents": "[Purification of microbial asparaginases by the aid of affinity chromatography]. Asparaginases from Escherichia coli and Erwinia caratovora were isolated and purified by column chromatography with a specific sorbent (sepharose, covalently bound to N-alpha-(6-aminohexyl)-D-asparagine). Homogenous asparaginase from E. coli was isolated by one-step procedure, while the enzyme from Er. carotovara was 50-60-fold purified. Asparaginase from Mycobacterium n. sp. was found not to bind with the specific sorbent."}
{"id": "PMID:1095079", "title": "[Binding of ribosomal RNAs with ribosomal proteins covalently bound to polymer carriers].", "content": "Conditions for covalent binding of ribosome proteins from Escherichia coli with insoluble polymers are found. A number of polymer carriers and several methods of protein binding to them were tested, the best one being the fixation of ribosome proteins on cyanogen bromide-activated Sepharose. Binding is studied with fixed on the polymer total 30S and 50S proteins, with individual S7 and S20 proteins, 16S and 23S RNAs in conditions optimal for the reconstruction of 30S subunits in vitro. It is found that at least some of ribosome proteins, being bound with polymer carrier, retain the ability to recognize their specific sites on RNA.", "contents": "[Binding of ribosomal RNAs with ribosomal proteins covalently bound to polymer carriers]. Conditions for covalent binding of ribosome proteins from Escherichia coli with insoluble polymers are found. A number of polymer carriers and several methods of protein binding to them were tested, the best one being the fixation of ribosome proteins on cyanogen bromide-activated Sepharose. Binding is studied with fixed on the polymer total 30S and 50S proteins, with individual S7 and S20 proteins, 16S and 23S RNAs in conditions optimal for the reconstruction of 30S subunits in vitro. It is found that at least some of ribosome proteins, being bound with polymer carrier, retain the ability to recognize their specific sites on RNA."}
{"id": "PMID:1095090", "title": "Comparative evaluation of artificial ventricles in the United States.", "content": "A cooperative, comparative evaluation of nine artificial ventricles was performed on two standardized mock circulations. The ventricles included five air-driven diaphragm types, three sack types and a mechanically driven type. The slopes of the ventricular output curves varied from 0.04 to 0.88 at 0 to 5 Torr filling pressure and the maximum ventricular output varied from 3.8 to 11.9 liters/min at 100 Torr outflow pressure. All ventricles had decreased output with increased outflow pressures (70 to 130 Torr). Hemolysis index ratio (HI test/HI std) for HI std equals 0.024 plus or minus .005 (plus or minus 1 SD) g/100 liters (N equals 12), was +21.5 and +6.9 for a Dacron cloth and fibril heart, respectively, +2.0 to +2.86 for three sack ventricles, and +3.2 for a smooth diaphragm ventricle. The mechanical ventricle with a sinusoidal driving waveform and smooth surface had the lowest hemolysis, HI equals 0.008 plus or minus 0.003 (plus or minus 1 SD) (N equals 6). Sack ventricles caused marked hemolysis if the walls touched during systole. Ventricular dimensions varied: weight 116 to 700 g, length 9.2 to 18.7 cm, and volume 235 to 430 ml. Performance data was returned to each individual laboratory which resulted in modification of ventricular design in at least three instances. Comparative, standardized testing of artificial ventricles may shorten development time and provide performance criteria for application in man.", "contents": "Comparative evaluation of artificial ventricles in the United States. A cooperative, comparative evaluation of nine artificial ventricles was performed on two standardized mock circulations. The ventricles included five air-driven diaphragm types, three sack types and a mechanically driven type. The slopes of the ventricular output curves varied from 0.04 to 0.88 at 0 to 5 Torr filling pressure and the maximum ventricular output varied from 3.8 to 11.9 liters/min at 100 Torr outflow pressure. All ventricles had decreased output with increased outflow pressures (70 to 130 Torr). Hemolysis index ratio (HI test/HI std) for HI std equals 0.024 plus or minus .005 (plus or minus 1 SD) g/100 liters (N equals 12), was +21.5 and +6.9 for a Dacron cloth and fibril heart, respectively, +2.0 to +2.86 for three sack ventricles, and +3.2 for a smooth diaphragm ventricle. The mechanical ventricle with a sinusoidal driving waveform and smooth surface had the lowest hemolysis, HI equals 0.008 plus or minus 0.003 (plus or minus 1 SD) (N equals 6). Sack ventricles caused marked hemolysis if the walls touched during systole. Ventricular dimensions varied: weight 116 to 700 g, length 9.2 to 18.7 cm, and volume 235 to 430 ml. Performance data was returned to each individual laboratory which resulted in modification of ventricular design in at least three instances. Comparative, standardized testing of artificial ventricles may shorten development time and provide performance criteria for application in man."}
{"id": "PMID:1095094", "title": "Mediterranean macrothrombocytopenia.", "content": "Platelet count, platelet size, and circulating platelet biomass concentration estimates made with an erythrocyte-calibrated electronic sizing system on EDTA-anticoagulated blood samples gave population medians and 95% ranges for 145 asymptomatic Mediterranean and 200 healthy Northern European subjects. The Mediterraneans had lower platelet counts [161,000 (89,000-290,000)/mul compared with 219,000 (148,000-323,000)/mul] and higher arithmetic mean volumes [17.8 (10.8-29.2) cu mum compared with 12.4 (9.9-15.6) cu mum], while the individual lognormal platelet size distribution profiles were comparable [geomatric standard deviations of 1.78 (1.60-1.98) against 1.70 (1.54-1.88)]; and the platelet biomass concentrations, given by count per microliter times mean volume times 10- minus 7 and expressed as a volumetric percentage of whole blood, were almost identical [0.286% (0.216%-0.379%) against 0.272% (0.201%-0.367%)]. Mediterranean macrothrombocytopenia is, therefore, considered a benign morphologic variant that requires differentiation from thrombocytopenias in which the circulating platelet biomass concentration is decreased.", "contents": "Mediterranean macrothrombocytopenia. Platelet count, platelet size, and circulating platelet biomass concentration estimates made with an erythrocyte-calibrated electronic sizing system on EDTA-anticoagulated blood samples gave population medians and 95% ranges for 145 asymptomatic Mediterranean and 200 healthy Northern European subjects. The Mediterraneans had lower platelet counts [161,000 (89,000-290,000)/mul compared with 219,000 (148,000-323,000)/mul] and higher arithmetic mean volumes [17.8 (10.8-29.2) cu mum compared with 12.4 (9.9-15.6) cu mum], while the individual lognormal platelet size distribution profiles were comparable [geomatric standard deviations of 1.78 (1.60-1.98) against 1.70 (1.54-1.88)]; and the platelet biomass concentrations, given by count per microliter times mean volume times 10- minus 7 and expressed as a volumetric percentage of whole blood, were almost identical [0.286% (0.216%-0.379%) against 0.272% (0.201%-0.367%)]. Mediterranean macrothrombocytopenia is, therefore, considered a benign morphologic variant that requires differentiation from thrombocytopenias in which the circulating platelet biomass concentration is decreased."}
{"id": "PMID:1095095", "title": "Selected list of books and journals for the small medical library.", "content": "This revised list of 446 books and 137 journals is intended as a selection guide for small or medium-sized hospital libraries or for the small medical library serving a specified clientele. Books and journals are categorized by subject, with the books being followed by an author index and the journals by an alphabetical title listing. Items suggested for initial purchase by smaller libraries are indicated by an asterisk. To purchase the entire collection of books and to pay for annual subscriptions to all the journals would require an expenditure for about $14,500. The cost of only the asterisked items recommended for first purchase totals approximately $4,100.", "contents": "Selected list of books and journals for the small medical library. This revised list of 446 books and 137 journals is intended as a selection guide for small or medium-sized hospital libraries or for the small medical library serving a specified clientele. Books and journals are categorized by subject, with the books being followed by an author index and the journals by an alphabetical title listing. Items suggested for initial purchase by smaller libraries are indicated by an asterisk. To purchase the entire collection of books and to pay for annual subscriptions to all the journals would require an expenditure for about $14,500. The cost of only the asterisked items recommended for first purchase totals approximately $4,100."}
{"id": "PMID:1095096", "title": "Science-specialty literatures their legendary contemporary polarity, based on the transmission of information between generations.", "content": "Each of fifteen scientific specialty fields was represented by a journal and the percent of \"legendary\" papers (those with at least one reference twenty-five or more years old) was calculated for each field. Non-legendary papers were designated as \"contemporary\". A historical divergence was uncovered when the highly legendary field, physiology, was compared with the highly contemporary field, microbiology, both fields being moderately contemporary in 1922. The legendary contemporary field characteristics of a field are useful for estimating libraries' requirements for back holdings and for appraising literature searches.", "contents": "Science-specialty literatures their legendary contemporary polarity, based on the transmission of information between generations. Each of fifteen scientific specialty fields was represented by a journal and the percent of \"legendary\" papers (those with at least one reference twenty-five or more years old) was calculated for each field. Non-legendary papers were designated as \"contemporary\". A historical divergence was uncovered when the highly legendary field, physiology, was compared with the highly contemporary field, microbiology, both fields being moderately contemporary in 1922. The legendary contemporary field characteristics of a field are useful for estimating libraries' requirements for back holdings and for appraising literature searches."}
{"id": "PMID:1095097", "title": "Past presidents I have known.", "content": "This paper is an account of the accomplishments of some of the early past presidents of the Medical Library Association known personally to the author in his career as a medical librarian. It demonstrates the qualities that made these librarians leaders of our profession and also indicates their personal attributes that contributed to the advancement of medical librarianship. It is hoped that the historical presentation of some of the giants of our profession will inspire present and future presidents and other medical librarians with an understanding of some of the qualities necessary to the continuing success of our profession.", "contents": "Past presidents I have known. This paper is an account of the accomplishments of some of the early past presidents of the Medical Library Association known personally to the author in his career as a medical librarian. It demonstrates the qualities that made these librarians leaders of our profession and also indicates their personal attributes that contributed to the advancement of medical librarianship. It is hoped that the historical presentation of some of the giants of our profession will inspire present and future presidents and other medical librarians with an understanding of some of the qualities necessary to the continuing success of our profession."}
{"id": "PMID:1095098", "title": "Comparison of skin erythemas under two different fractionation schemes.", "content": "This paper reports the results of a prospective clinical trial comparing 200 rads daily five times weekly for four weeks with 400 rads twice weekly for four weeks. Total dose in the first case was 4,000 rads and in the second 3,200 rads. Thirty patients threated palliatively for bronchial carcinoma are involved in this report. Treatment was given at 220 kV and the intensity of skin erythema at the end of the treatment was the measure of comparison. Generally 200 rads daily five times weekly for four weeks produced more intense erythema than the other scheme used.", "contents": "Comparison of skin erythemas under two different fractionation schemes. This paper reports the results of a prospective clinical trial comparing 200 rads daily five times weekly for four weeks with 400 rads twice weekly for four weeks. Total dose in the first case was 4,000 rads and in the second 3,200 rads. Thirty patients threated palliatively for bronchial carcinoma are involved in this report. Treatment was given at 220 kV and the intensity of skin erythema at the end of the treatment was the measure of comparison. Generally 200 rads daily five times weekly for four weeks produced more intense erythema than the other scheme used."}
{"id": "PMID:1095101", "title": "Whole body potassium in patients with ureterosigmoid anastomoses.", "content": "Total body potassium was measured by whole body counting in 11 patients with uretero-sigmoid anastomoses who had undergone cystectomy 4 months to 13 years previously. The measured total body potassium was not significantly different in any of the patients from the \"normal\" value for the individual patients, predicted from height and age. It was only significantly different from that predicted from height, age and weight in 1 patient. In the series as a whole, the measured values of whole body potassium were not significantly different from the \"normal\" values estimated by either relationship. From measurements of skinfold thickness, the lean body mass of each patient was estimated. The mean potassium content, 62-0 mEg/kg LBM in the male patients was within the normal range. The absence of potassium depletion may derive from the successful abolition of hyperchloraemic acidosis by long-term alkali therapy.", "contents": "Whole body potassium in patients with ureterosigmoid anastomoses. Total body potassium was measured by whole body counting in 11 patients with uretero-sigmoid anastomoses who had undergone cystectomy 4 months to 13 years previously. The measured total body potassium was not significantly different in any of the patients from the \"normal\" value for the individual patients, predicted from height and age. It was only significantly different from that predicted from height, age and weight in 1 patient. In the series as a whole, the measured values of whole body potassium were not significantly different from the \"normal\" values estimated by either relationship. From measurements of skinfold thickness, the lean body mass of each patient was estimated. The mean potassium content, 62-0 mEg/kg LBM in the male patients was within the normal range. The absence of potassium depletion may derive from the successful abolition of hyperchloraemic acidosis by long-term alkali therapy."}
{"id": "PMID:1095102", "title": "Oestrogen therapy and serum cortisol in carcinoma of the prostate.", "content": "Patients with prostatic cancer, receiving oestrogen therapy were found to have elevated serum cortisol levels and lymphopenia. Oestrogens are known to have a profound effect on the immune system; the possibility that this effect is mediated through the adrenal cortex is discussed. The significance of these findings, however, remains to be determined.", "contents": "Oestrogen therapy and serum cortisol in carcinoma of the prostate. Patients with prostatic cancer, receiving oestrogen therapy were found to have elevated serum cortisol levels and lymphopenia. Oestrogens are known to have a profound effect on the immune system; the possibility that this effect is mediated through the adrenal cortex is discussed. The significance of these findings, however, remains to be determined."}
{"id": "PMID:1095103", "title": "Serial Fluorine-18 bone scans in the follow-up of carcinoma of the prostate.", "content": "74 patients with prostatic cancer were studied annually by combined radiological and fluorine-18 scan survey over a 5-year period. The results of the long term follow-up of bone cans is reported. At the time of the initial diagnosis 71-5% of the patients had advanced disease and 56% had radiological or scan evidence of metastases. A critical evaluation of the scans resulted in the detection of early bone lesions in 25% of patients with no radiological evidence of metastases. Follow-up of these patients has shown that scan abnormalities preceded radiological changes from between 1 to 4 years and there was good correlation proven histologically by bone biopsy or autopsy in more than half of the patients. In patients with a positive bone scan and positive X-rays the scan abnormalities were more extensive than the corresponding X-ray lesions. When bone healing occurred with endocrine treatment this was more readily apparent on the X-rays. False negative scans were not seen with fluorine-18 which allows for greater accuracy in the detection of skeletal metastases. Bone scanning has enabled correct staging to be carried out. This study confirms the high incidence of cardiac and vascular complications in patients treated with oestrogens.", "contents": "Serial Fluorine-18 bone scans in the follow-up of carcinoma of the prostate. 74 patients with prostatic cancer were studied annually by combined radiological and fluorine-18 scan survey over a 5-year period. The results of the long term follow-up of bone cans is reported. At the time of the initial diagnosis 71-5% of the patients had advanced disease and 56% had radiological or scan evidence of metastases. A critical evaluation of the scans resulted in the detection of early bone lesions in 25% of patients with no radiological evidence of metastases. Follow-up of these patients has shown that scan abnormalities preceded radiological changes from between 1 to 4 years and there was good correlation proven histologically by bone biopsy or autopsy in more than half of the patients. In patients with a positive bone scan and positive X-rays the scan abnormalities were more extensive than the corresponding X-ray lesions. When bone healing occurred with endocrine treatment this was more readily apparent on the X-rays. False negative scans were not seen with fluorine-18 which allows for greater accuracy in the detection of skeletal metastases. Bone scanning has enabled correct staging to be carried out. This study confirms the high incidence of cardiac and vascular complications in patients treated with oestrogens."}
{"id": "PMID:1095105", "title": "The concentration of sulphamethoxazole and trimethoprim in human prostate gland.", "content": "Sulphamethoxazole and trimethoprim concentrations were measured in 19 enucleated human prostate glands. Good concentrations of sulphamethoxazole were found after 4 days of treatment: the ratio of plasma concentration to that of prostate varied from 1-25 to 3-29. The trimethoprim concentrations were 2 to 3 times higher in the prostate than in the plasma. These high concentrations were reached very rapidly after the first dose of trimethoprim. The results are compared with date from the literature. The combination sulphamethoxazole-trimethoprim should be of value in the treatment of bacterial prostatitis.", "contents": "The concentration of sulphamethoxazole and trimethoprim in human prostate gland. Sulphamethoxazole and trimethoprim concentrations were measured in 19 enucleated human prostate glands. Good concentrations of sulphamethoxazole were found after 4 days of treatment: the ratio of plasma concentration to that of prostate varied from 1-25 to 3-29. The trimethoprim concentrations were 2 to 3 times higher in the prostate than in the plasma. These high concentrations were reached very rapidly after the first dose of trimethoprim. The results are compared with date from the literature. The combination sulphamethoxazole-trimethoprim should be of value in the treatment of bacterial prostatitis."}
{"id": "PMID:1095106", "title": "Vas anastomosis: a return to simplicity.", "content": "Vas anastomosis is successful in restoring sperm to the ejaculate in 80 to 90% of cases. A simple, end-to-end, mucosa-to-mucosa anastomosis performed with non-absorbable, monofilament sutures is, in the author's opinion, the best procedure. Pregnancies follow in about one-third of the cases.", "contents": "Vas anastomosis: a return to simplicity. Vas anastomosis is successful in restoring sperm to the ejaculate in 80 to 90% of cases. A simple, end-to-end, mucosa-to-mucosa anastomosis performed with non-absorbable, monofilament sutures is, in the author's opinion, the best procedure. Pregnancies follow in about one-third of the cases."}
{"id": "PMID:1095107", "title": "Ampicillin, carbenicillin indanyl ester, and nifuratel in the treatment of urinary infection in domiciliary practice.", "content": "A total of 120 patients, including 53 pregnant women with significant bacteriuria, received 163 7-day courses of oral antimicrobial agents allocated in a randomised manner. The cure rates after 6 weeks' follow-up ranged from 73% to 86%, and there was no statistical difference between preparations of ampicillin, carbenicillin indanyl easter, and 2 different formulations of nifuratel. Side-effects occurred in 30% to 40% of the courses of penicillin drugs, but in under 15% of the course of nifuratel. It is concluded that the new oral preparation of carbenicillin is a useful addition to the list of antimicrobial agents which are effective in the treatment of urinary infections in domiciliary patients. Furthermore, nifuratel has been confirmed as a highly active non-toxic drug which is valuable in the treatment of urinary infections.", "contents": "Ampicillin, carbenicillin indanyl ester, and nifuratel in the treatment of urinary infection in domiciliary practice. A total of 120 patients, including 53 pregnant women with significant bacteriuria, received 163 7-day courses of oral antimicrobial agents allocated in a randomised manner. The cure rates after 6 weeks' follow-up ranged from 73% to 86%, and there was no statistical difference between preparations of ampicillin, carbenicillin indanyl easter, and 2 different formulations of nifuratel. Side-effects occurred in 30% to 40% of the courses of penicillin drugs, but in under 15% of the course of nifuratel. It is concluded that the new oral preparation of carbenicillin is a useful addition to the list of antimicrobial agents which are effective in the treatment of urinary infections in domiciliary patients. Furthermore, nifuratel has been confirmed as a highly active non-toxic drug which is valuable in the treatment of urinary infections."}
{"id": "PMID:1095108", "title": "Intra-arterial infusion of Solcoseryl: a clinical trial of a method of treatment for pre-gangrene of the lower limb.", "content": "A randomized double blind trial of the drug Solcoseryl given by intra-arterial infusion was carried out on 57 patients with pre-gangrene of the lower limb. A sequential analysis was carried out and the trial stopped when the results showed a statistically significant result in favour of the active drug.", "contents": "Intra-arterial infusion of Solcoseryl: a clinical trial of a method of treatment for pre-gangrene of the lower limb. A randomized double blind trial of the drug Solcoseryl given by intra-arterial infusion was carried out on 57 patients with pre-gangrene of the lower limb. A sequential analysis was carried out and the trial stopped when the results showed a statistically significant result in favour of the active drug."}
{"id": "PMID:1095109", "title": "The construction of a skin-lined hemithorax in pigs.", "content": "The feasibility of creating a skin-lined hemithorax was tested in 6 pigs. All the pigs survived intrapleural skin grafting, and within 3 weeks they doubled their mean initial weight from 16-3 plus or minus 1-6 kg to 32-4 plus or minus 2-6 kg. In 4 (out of 4) pigs the skin-lined cavity was opened successfully to the exterior; it had reproduced closely the contour of the hemithorax. It is concluded that it is feasible to construct a skin-lined hemithorax with a mean volume of 276-3 plus or minus 19-6 ml in pigs, whose mean body weigh was 37-5 plus or minus 2.5 kg. This confirms earlier work in the rat.", "contents": "The construction of a skin-lined hemithorax in pigs. The feasibility of creating a skin-lined hemithorax was tested in 6 pigs. All the pigs survived intrapleural skin grafting, and within 3 weeks they doubled their mean initial weight from 16-3 plus or minus 1-6 kg to 32-4 plus or minus 2-6 kg. In 4 (out of 4) pigs the skin-lined cavity was opened successfully to the exterior; it had reproduced closely the contour of the hemithorax. It is concluded that it is feasible to construct a skin-lined hemithorax with a mean volume of 276-3 plus or minus 19-6 ml in pigs, whose mean body weigh was 37-5 plus or minus 2.5 kg. This confirms earlier work in the rat."}
{"id": "PMID:1095110", "title": "Diagnosis, clinical course and treatment of acute dermal gangrene.", "content": "Twenty patients with acute dermal gangrene following surgery, trauma or sepsis are described. In 12 the skin became gangrenous secondary to a necrotizing process affecting the subdermal fascia, and in 8 the condition arose primarily in the skin. In the first group mortality was high unless radical excision of the necrotic fascia was performed at an early stage; in 3 of the recent patients the overlying skin was removed, defatted and stored for later grafting. In the second group, incision and adequate drainage combined with antibiotics seemed to suffice. Hyperbaric oxygen was of dubious value in the first group but appeared to contribute to arrest of the lesion in the second group.", "contents": "Diagnosis, clinical course and treatment of acute dermal gangrene. Twenty patients with acute dermal gangrene following surgery, trauma or sepsis are described. In 12 the skin became gangrenous secondary to a necrotizing process affecting the subdermal fascia, and in 8 the condition arose primarily in the skin. In the first group mortality was high unless radical excision of the necrotic fascia was performed at an early stage; in 3 of the recent patients the overlying skin was removed, defatted and stored for later grafting. In the second group, incision and adequate drainage combined with antibiotics seemed to suffice. Hyperbaric oxygen was of dubious value in the first group but appeared to contribute to arrest of the lesion in the second group."}
{"id": "PMID:1095111", "title": "Wound infections due to Bacteroides fragilis following intestinal surgery.", "content": "Ninety-six wound infections due to Bacteroides fragilis occurring after intestinal surgery are described. Most infections followed appendicectomy or colectomy for diverticulitis. B. fragilis was isolated in pure growth from 47 per cent of infections, and in mixed culture the other common bacteria were Klebsiella spp., Escherichia coli and Enterobacter spp. Fifty-three per cent of the infections responded rapidly to either non-specific measures or antibiotic therapy, but 47 per cent of patients developed complications, usually abscess formation. The successful isolation of bacteroides from clinical material requires special precautions. The specimen for bacteriological examination must be sent to the laboratory in a transport medium which maintains an anaerobic environment and the culture techniques must include a selective agar for the isolation of anaerobic bacteria. The role of antibiotic therapy in preventing postoperative wound infection after intestinal surgery is discussed. Bacteroides has an unusualy pattern of antibiotic susceptibility and it is essential that an effective antibiotic such as lincomycin is included in the treatment of infection originating from the intestinal tract.", "contents": "Wound infections due to Bacteroides fragilis following intestinal surgery. Ninety-six wound infections due to Bacteroides fragilis occurring after intestinal surgery are described. Most infections followed appendicectomy or colectomy for diverticulitis. B. fragilis was isolated in pure growth from 47 per cent of infections, and in mixed culture the other common bacteria were Klebsiella spp., Escherichia coli and Enterobacter spp. Fifty-three per cent of the infections responded rapidly to either non-specific measures or antibiotic therapy, but 47 per cent of patients developed complications, usually abscess formation. The successful isolation of bacteroides from clinical material requires special precautions. The specimen for bacteriological examination must be sent to the laboratory in a transport medium which maintains an anaerobic environment and the culture techniques must include a selective agar for the isolation of anaerobic bacteria. The role of antibiotic therapy in preventing postoperative wound infection after intestinal surgery is discussed. Bacteroides has an unusualy pattern of antibiotic susceptibility and it is essential that an effective antibiotic such as lincomycin is included in the treatment of infection originating from the intestinal tract."}
{"id": "PMID:1095112", "title": "\"Topical nephropathy\" and \"tropical extramembranous glomerulonephritis\" of unknown aetiology in Senegal.", "content": "A study of renal biopsy specimens obtained in Senegal from 24 children and six adults with nephrotic syndrome showed two unusual varieties of nephropathy--namely, an extramembranous glomerulonephritis associated with hypocomplementaemia (four cases), a combination previously described only in systemic lupus erythematosus, and a \"tropical nephropathy\" (16 cases). The latter, though lacking the diffuse glomerular deposits of immunoglobulin described in quartan malarial nephropathy (Q.M.N.), showed a curious progressive and segmental glomerulosclerosis, characterized by a \"flaking\" or fibrillary splitting of the glomerular capillary wall, seen in Q.M.N. Serological evidence of malaria was lacking in a third of the childhood cases.", "contents": "\"Topical nephropathy\" and \"tropical extramembranous glomerulonephritis\" of unknown aetiology in Senegal. A study of renal biopsy specimens obtained in Senegal from 24 children and six adults with nephrotic syndrome showed two unusual varieties of nephropathy--namely, an extramembranous glomerulonephritis associated with hypocomplementaemia (four cases), a combination previously described only in systemic lupus erythematosus, and a \"tropical nephropathy\" (16 cases). The latter, though lacking the diffuse glomerular deposits of immunoglobulin described in quartan malarial nephropathy (Q.M.N.), showed a curious progressive and segmental glomerulosclerosis, characterized by a \"flaking\" or fibrillary splitting of the glomerular capillary wall, seen in Q.M.N. Serological evidence of malaria was lacking in a third of the childhood cases."}
{"id": "PMID:1095113", "title": "Mitigation of amphotericin B nephrotoxicity by mannitol.", "content": "Renal transplant recipients are susceptible to a number of fungal infections amenable to therapy with amphotericin B, but azotaemia is an almost invariable sequel to the use of this agent. As intravenous mannitol has been shown to minimize nephrotoxicity induced by amphotericin B in dogs we treated four kidney transplant recipients who had systemic fungal infections with mannitol and amphotericin B. None showed significant reduction in renal function though a mild metabolic acidosis did develop.", "contents": "Mitigation of amphotericin B nephrotoxicity by mannitol. Renal transplant recipients are susceptible to a number of fungal infections amenable to therapy with amphotericin B, but azotaemia is an almost invariable sequel to the use of this agent. As intravenous mannitol has been shown to minimize nephrotoxicity induced by amphotericin B in dogs we treated four kidney transplant recipients who had systemic fungal infections with mannitol and amphotericin B. None showed significant reduction in renal function though a mild metabolic acidosis did develop."}
{"id": "PMID:1095114", "title": "First results of a randomized clinical trial of fast neutrons compared with X or gamma rays in treatment of advanced tumours of the head and neck. Report to the Medical Research Council.", "content": "Results of the first randomized clinical trial to compare the effects of fast neutrons and those of x or gamma rays (photons) in treating patients with advanced tumours of the head and neck are reported. In 37 out of 52 patients treated with neutrons and 16 out of 50 treated with photons the local tumour completely regressed; the tumour later recurred in nine of the 16 photon patients but in none of the 37 neutron patients. The advantages to the neutron-treated patients were seen in tumours of well and poorly differentiated histology and in each site. Complications after treatment did not differ significantly between the groups. Despite these substantial differences in local control of the tumour there were no significant differences in mortality between the series. A detailed study of the effective doses and the response of tumours and normal tissue in each series indicated that the improved results from neutron therapy were due to differences in the biological quality of the beam and not to the rather higher average effective dose in the neutron series. To assess the long-term effects of neutron treatment patients in earlier stages of disease and with smaller tumours should be included in the next phase of the trial.", "contents": "First results of a randomized clinical trial of fast neutrons compared with X or gamma rays in treatment of advanced tumours of the head and neck. Report to the Medical Research Council. Results of the first randomized clinical trial to compare the effects of fast neutrons and those of x or gamma rays (photons) in treating patients with advanced tumours of the head and neck are reported. In 37 out of 52 patients treated with neutrons and 16 out of 50 treated with photons the local tumour completely regressed; the tumour later recurred in nine of the 16 photon patients but in none of the 37 neutron patients. The advantages to the neutron-treated patients were seen in tumours of well and poorly differentiated histology and in each site. Complications after treatment did not differ significantly between the groups. Despite these substantial differences in local control of the tumour there were no significant differences in mortality between the series. A detailed study of the effective doses and the response of tumours and normal tissue in each series indicated that the improved results from neutron therapy were due to differences in the biological quality of the beam and not to the rather higher average effective dose in the neutron series. To assess the long-term effects of neutron treatment patients in earlier stages of disease and with smaller tumours should be included in the next phase of the trial."}
{"id": "PMID:1095115", "title": "Use of computer program for diagnosing jaundice in district hospitals and specialized liver unit.", "content": "A computer-assisted model for diagnosing jaundice has been adapted for use on the University of London C.D.C. 7600 computer via an on-line terminal at King's College Hospital to provide a rapid turn-round time. The model was used prospectively in the diagnosis of 219 patients--135 seen in a specialized liver unit and 84 seen in one of four district hospitals in south-east London--with an overall accuracy in distinguishing among 11 different causes of jaundice of 69% and 62% respectively. These figures rose to 77% and 88% respectively when only those patients in whom the final diagnosis reached a \"certain\" probability were considered. When used to distinguish between a medical and a surgical cause of jaundice the accuracy was 86% in the liver unit and 77% in the district hospitals, rising to 95% in both series for those with a diagnosis of certain probability. The proposed improvements to the model--namely, the use of two deparate data bases and more diagnoses within the matrix--should be improve the accuracy even further. In practice the rapid feedback to the clinicians looking after patients provided help in managing difficult cases.", "contents": "Use of computer program for diagnosing jaundice in district hospitals and specialized liver unit. A computer-assisted model for diagnosing jaundice has been adapted for use on the University of London C.D.C. 7600 computer via an on-line terminal at King's College Hospital to provide a rapid turn-round time. The model was used prospectively in the diagnosis of 219 patients--135 seen in a specialized liver unit and 84 seen in one of four district hospitals in south-east London--with an overall accuracy in distinguishing among 11 different causes of jaundice of 69% and 62% respectively. These figures rose to 77% and 88% respectively when only those patients in whom the final diagnosis reached a \"certain\" probability were considered. When used to distinguish between a medical and a surgical cause of jaundice the accuracy was 86% in the liver unit and 77% in the district hospitals, rising to 95% in both series for those with a diagnosis of certain probability. The proposed improvements to the model--namely, the use of two deparate data bases and more diagnoses within the matrix--should be improve the accuracy even further. In practice the rapid feedback to the clinicians looking after patients provided help in managing difficult cases."}
{"id": "PMID:1095116", "title": "Use of biochemical profile in children's hospital: results of two controlled trials.", "content": "Two controlled trials of the use of a biochemical profile were conducted in a childern's hospital to see whether the profile led to diagnoses which would not otherwise [have been made and to see what effect it had on the number of extra  requests for pathololgical investigations and the length of stay in hospital]. Altogether 2816 children were examined and 13 new diagnoses made. There was a significant increases in the total number of pathorequests but the profile did not alter the length of stay in hospital. We conclude that the profile mad only a small contribtuion to the overall care of the pateints.", "contents": "Use of biochemical profile in children's hospital: results of two controlled trials. Two controlled trials of the use of a biochemical profile were conducted in a childern's hospital to see whether the profile led to diagnoses which would not otherwise [have been made and to see what effect it had on the number of extra  requests for pathololgical investigations and the length of stay in hospital]. Altogether 2816 children were examined and 13 new diagnoses made. There was a significant increases in the total number of pathorequests but the profile did not alter the length of stay in hospital. We conclude that the profile mad only a small contribtuion to the overall care of the pateints."}
{"id": "PMID:1095118", "title": "Diagnosis of gastric cancer.", "content": "A prospective comparison was made of the accuracy of different diagnostic methods for gastric cancer. The basis of the study was a consecutive series of 113 patients thought to have gastric pathology; cancer was the final diagnosis in 32. Endoscopy and radiology were the most accurate investigations, whereas biopsy, cytology, and clinical examination gave disappointing results. A wide range of clinical features and laboratory investigations were studied in all patients in an attempt to identify criteria suggestive of malignancy. Multifactorial computer analysis of these investigations failed to improve upon the radiological diagnosis. A systemic approach designed to make optimal use of limited endoscopic and histopathological resources in the diagnosis of gastric lesions is presented.", "contents": "Diagnosis of gastric cancer. A prospective comparison was made of the accuracy of different diagnostic methods for gastric cancer. The basis of the study was a consecutive series of 113 patients thought to have gastric pathology; cancer was the final diagnosis in 32. Endoscopy and radiology were the most accurate investigations, whereas biopsy, cytology, and clinical examination gave disappointing results. A wide range of clinical features and laboratory investigations were studied in all patients in an attempt to identify criteria suggestive of malignancy. Multifactorial computer analysis of these investigations failed to improve upon the radiological diagnosis. A systemic approach designed to make optimal use of limited endoscopic and histopathological resources in the diagnosis of gastric lesions is presented."}
{"id": "PMID:1095121", "title": "Comparative trial of nafoxidine and ethinyloestradiol in advanced breast cancer: an E.O.R.T.C. study.", "content": "A randomized clinical trial of nafoxidine, a non-steroidal oestrogen antagonist, and ethinyloestradiol in postmenopausal patients with advanced breast cancer produced objective remissions in 31% of 49 women receiving nafoxidine and in 14% of 49 receiving ethinyloestradiol. The differences in remission rates was almost significant (0.05 less than P less than 0.10). Life-threatening complications were more frequent with ethinyloestradiol than with nafoxidine but the latter produced specific toxic reactions on skin and hair that may limit its practical usefulness. Synthetic oestrogen antagonists may occupy a privileged place in the treatment of breast cancer, and other representatives of this new class of compounds should be accurately assessed in randomized clinical trials.", "contents": "Comparative trial of nafoxidine and ethinyloestradiol in advanced breast cancer: an E.O.R.T.C. study. A randomized clinical trial of nafoxidine, a non-steroidal oestrogen antagonist, and ethinyloestradiol in postmenopausal patients with advanced breast cancer produced objective remissions in 31% of 49 women receiving nafoxidine and in 14% of 49 receiving ethinyloestradiol. The differences in remission rates was almost significant (0.05 less than P less than 0.10). Life-threatening complications were more frequent with ethinyloestradiol than with nafoxidine but the latter produced specific toxic reactions on skin and hair that may limit its practical usefulness. Synthetic oestrogen antagonists may occupy a privileged place in the treatment of breast cancer, and other representatives of this new class of compounds should be accurately assessed in randomized clinical trials."}
{"id": "PMID:1095122", "title": "Controlled clinical trial of L-dopa and nafoxidine in advanced breast cancer: an E.O.R.T.C. study.", "content": "L-Dopa lowers plasma prolactin levels, and there have been reports that patients with advanced breast cancer have been successfully treated with L-dopa. To test the potential value of L-dopa in this disease a randomized clinical trial of L-dopa and nafoxidine (as the reference compound) was conducted in postmenopausal women with advanced breast cancer. Objective remissions were obtained in sever out of 36 patients (19%) treated with nafoxidine but in none out of 40 patients treated with L-dopa. L-Dopa in the dose schedule used seems to be ineffective in advanced breast cancer.", "contents": "Controlled clinical trial of L-dopa and nafoxidine in advanced breast cancer: an E.O.R.T.C. study. L-Dopa lowers plasma prolactin levels, and there have been reports that patients with advanced breast cancer have been successfully treated with L-dopa. To test the potential value of L-dopa in this disease a randomized clinical trial of L-dopa and nafoxidine (as the reference compound) was conducted in postmenopausal women with advanced breast cancer. Objective remissions were obtained in sever out of 36 patients (19%) treated with nafoxidine but in none out of 40 patients treated with L-dopa. L-Dopa in the dose schedule used seems to be ineffective in advanced breast cancer."}
{"id": "PMID:1095132", "title": "Duration of treatment for urinary tract infections in children.", "content": "In a double-blind trial 45 children aged 6 months to 14 years with Escherichia coli infections of the urinary tract were given co-trimoxazole for two weeks and then allotted at random to one of two treatment groups for the remainder of six months; one continued with the active drug and the other with dummy tablets of identical appearance. Of the 24 children who took co-trimoxazole for two weeks and the 21 who took it for six months, 11 and 10, respectively, remained without further infections for at least a year. Over 90% of the reinfections occurred within five months of stopping the antibiotics, and the longer treatment did not cause any delay in their appearance. Thus probably a six-month course of treatment is no more likely to achieve a cure than a two-week course; nevertheless, no infection occurred during treatment, and there may be an advantage in continuing with antibiotics in small dosage.", "contents": "Duration of treatment for urinary tract infections in children. In a double-blind trial 45 children aged 6 months to 14 years with Escherichia coli infections of the urinary tract were given co-trimoxazole for two weeks and then allotted at random to one of two treatment groups for the remainder of six months; one continued with the active drug and the other with dummy tablets of identical appearance. Of the 24 children who took co-trimoxazole for two weeks and the 21 who took it for six months, 11 and 10, respectively, remained without further infections for at least a year. Over 90% of the reinfections occurred within five months of stopping the antibiotics, and the longer treatment did not cause any delay in their appearance. Thus probably a six-month course of treatment is no more likely to achieve a cure than a two-week course; nevertheless, no infection occurred during treatment, and there may be an advantage in continuing with antibiotics in small dosage."}
{"id": "PMID:1095133", "title": "Indomethacin--aspirin interaction: a clinical appraisal.", "content": "Plasma profiles of indomethacin after a 50-mg oral dose were constructed in six healthy volunteers before and after a week of aspirin treatment. Aspirin did not interfere with indomethacin plasma levels. To examine the clinical effect of concurrent indomethacin and aspirin treatment 20 patients with seropositive rheumatoid arthritis were given indomethacin 100 mg/day, aspirin soluble 4 g/day, and the two drugs taken together in random order. Analysis of the clinical indices of inflammation--articular index and mean pain score--and of the efficacy of each treatment showed no significant differences between the three treatment groups. With the proliferation in the number of anti-rheumatic drugs available, the case for giving two or more nonsteroidal anti-inflammatory drugs concurrently remains unproved.", "contents": "Indomethacin--aspirin interaction: a clinical appraisal. Plasma profiles of indomethacin after a 50-mg oral dose were constructed in six healthy volunteers before and after a week of aspirin treatment. Aspirin did not interfere with indomethacin plasma levels. To examine the clinical effect of concurrent indomethacin and aspirin treatment 20 patients with seropositive rheumatoid arthritis were given indomethacin 100 mg/day, aspirin soluble 4 g/day, and the two drugs taken together in random order. Analysis of the clinical indices of inflammation--articular index and mean pain score--and of the efficacy of each treatment showed no significant differences between the three treatment groups. With the proliferation in the number of anti-rheumatic drugs available, the case for giving two or more nonsteroidal anti-inflammatory drugs concurrently remains unproved."}
{"id": "PMID:1095134", "title": "Prophylactic fluoride treatment and aged bones.", "content": "In a double-blind trial with monofluorophosphate (25 mg fluoride per day) given to 460 aged persons (237 treated, 233 control) for eight months no difference was observed in height, admission to hospital, or mortality. Fractures and exacerbation of arthrosis were more frequent in the fluoride group. Vertebral x-ray films showed no difference. The free ionized fluoride levels in the plasma of the fluoride-treated group were still twice as high two months after treatment ended. Fluoride treatment in the prophylaxis of osteoporosis is not recommended unless there is simultaneous measurement of plasma ionized fluoride levels.", "contents": "Prophylactic fluoride treatment and aged bones. In a double-blind trial with monofluorophosphate (25 mg fluoride per day) given to 460 aged persons (237 treated, 233 control) for eight months no difference was observed in height, admission to hospital, or mortality. Fractures and exacerbation of arthrosis were more frequent in the fluoride group. Vertebral x-ray films showed no difference. The free ionized fluoride levels in the plasma of the fluoride-treated group were still twice as high two months after treatment ended. Fluoride treatment in the prophylaxis of osteoporosis is not recommended unless there is simultaneous measurement of plasma ionized fluoride levels."}
{"id": "PMID:1095135", "title": "Cryptococcal meningoencephalitis.", "content": "A patient was admitted to hospital with an apparent psychiatric disturbance. When she became stuporous the cerebrospinal fluid was cultured but proved sterile. The latex test showed that serum was positive for cryptococcal antigens, and cryptococcal meningoencephalitis was diagnosed. Amphotericin B was given but when she developed a toxic reaction it was replaced by flucytosine. She responded well to flucytosine alone and no side effects appeared on continued treatment. Cryptococcal meningitis may present as a psychiatric disturbance, and serological tests are invaluable aids to diagnosis.", "contents": "Cryptococcal meningoencephalitis. A patient was admitted to hospital with an apparent psychiatric disturbance. When she became stuporous the cerebrospinal fluid was cultured but proved sterile. The latex test showed that serum was positive for cryptococcal antigens, and cryptococcal meningoencephalitis was diagnosed. Amphotericin B was given but when she developed a toxic reaction it was replaced by flucytosine. She responded well to flucytosine alone and no side effects appeared on continued treatment. Cryptococcal meningitis may present as a psychiatric disturbance, and serological tests are invaluable aids to diagnosis."}
{"id": "PMID:1095137", "title": "Use of clomiphene and luteinizing hormone/follicle stimulating hormone-releasing hormone in investigation of ovulatory failure.", "content": "A luteinizing hormone/follicle-stimulating hormone-releasing hormone (LH/FSH-RH) test was performed in 70 women with amenorrhoea or anovulatory infertility, or both, and a clomiphene stimulation test was also performed in 24 of these patients. Most patients responded to LH/FSH-RH with significant increases in LH and FSH. In women with gonadal dysgenesis or premature ovarian failure exaggerated responses were observed after LH/FSH-RH and there was no change in high basal LH levels after clomiphene. Patients with absent or impaired responses to LH/FSH-RH failed to respond to clomiphene. All patients with anovulatory menstrual cycles responded to both LH/FSH-RH and clomiphene, while seven out of 13 amenorrhoeic patients with a normal LH/FSH-RH response showed an early LH rise during clomiphene treatment and six were unresponsive. These results suggest a difference between the two groups at hypothalamic level with consequent therapeutic implications.", "contents": "Use of clomiphene and luteinizing hormone/follicle stimulating hormone-releasing hormone in investigation of ovulatory failure. A luteinizing hormone/follicle-stimulating hormone-releasing hormone (LH/FSH-RH) test was performed in 70 women with amenorrhoea or anovulatory infertility, or both, and a clomiphene stimulation test was also performed in 24 of these patients. Most patients responded to LH/FSH-RH with significant increases in LH and FSH. In women with gonadal dysgenesis or premature ovarian failure exaggerated responses were observed after LH/FSH-RH and there was no change in high basal LH levels after clomiphene. Patients with absent or impaired responses to LH/FSH-RH failed to respond to clomiphene. All patients with anovulatory menstrual cycles responded to both LH/FSH-RH and clomiphene, while seven out of 13 amenorrhoeic patients with a normal LH/FSH-RH response showed an early LH rise during clomiphene treatment and six were unresponsive. These results suggest a difference between the two groups at hypothalamic level with consequent therapeutic implications."}
{"id": "PMID:1095143", "title": "A study of yaws among pygmies in Cameroon and Zaire.", "content": "In a survey of 333 pygmies in the Cameroon 4.5 percent. were found to have clinical evidence of yaws. This was also found in 10 per cent. of the inhabitants of camps in Zaire. The results of VDRL and FTA-ABS tests on a sample of the population are presented. These provide evidence of a very high incidence of treponemal disease, 80 to 90 percent. of the population showing serological evidence of infection. Active transmission is taking place in both areas but more frequently in Zaire.", "contents": "A study of yaws among pygmies in Cameroon and Zaire. In a survey of 333 pygmies in the Cameroon 4.5 percent. were found to have clinical evidence of yaws. This was also found in 10 per cent. of the inhabitants of camps in Zaire. The results of VDRL and FTA-ABS tests on a sample of the population are presented. These provide evidence of a very high incidence of treponemal disease, 80 to 90 percent. of the population showing serological evidence of infection. Active transmission is taking place in both areas but more frequently in Zaire."}
{"id": "PMID:1095145", "title": "Failure to infect gnotobiotic colostrum-deprived, and normal piglets with Treponema pallidum (Nichols).", "content": "Normal piglets kept at 16 degrees C. and gnotobiotic piglets kept at 32 degrees C. rapidly destroyed Treponema pallidum, while gnotobiotic piglets kept at 26 degrees C. showed slightly slower clearance. No infections were established. The high body temperature of the piglets has an important influence on the survival of T. pallidum.", "contents": "Failure to infect gnotobiotic colostrum-deprived, and normal piglets with Treponema pallidum (Nichols). Normal piglets kept at 16 degrees C. and gnotobiotic piglets kept at 32 degrees C. rapidly destroyed Treponema pallidum, while gnotobiotic piglets kept at 26 degrees C. showed slightly slower clearance. No infections were established. The high body temperature of the piglets has an important influence on the survival of T. pallidum."}
{"id": "PMID:1095144", "title": "Non-specific urethritis and the tetracyclines.", "content": "The possible aetiological factors in non-gonococcal and non-specific urethritis are reviewed. The response of NSU to various courses of different tetracycline drugs is assessed. Prolonged courses of treatment did not give better results than shorter courses. When reviewing the infective aetiology of non-gonococcal urethritis, it was noted that more than one organism (or potential pathogen) would be present in many cases. It is therefore surmised that there may be at times a mixed aetiology and at other times a truly non-specific aetiology. Isolations by different workers have indicated that the following organisms might be expected: Chlamydia 40 per cent.; Mycoplasma-M. hominis 20 per cent., T-strain over 60 per cent.; Trichomonas 15 per cent.; Candida possibly over 5 per cent. Truly non-specific urethritis may account for 25 to 30 per cent. of cases.", "contents": "Non-specific urethritis and the tetracyclines. The possible aetiological factors in non-gonococcal and non-specific urethritis are reviewed. The response of NSU to various courses of different tetracycline drugs is assessed. Prolonged courses of treatment did not give better results than shorter courses. When reviewing the infective aetiology of non-gonococcal urethritis, it was noted that more than one organism (or potential pathogen) would be present in many cases. It is therefore surmised that there may be at times a mixed aetiology and at other times a truly non-specific aetiology. Isolations by different workers have indicated that the following organisms might be expected: Chlamydia 40 per cent.; Mycoplasma-M. hominis 20 per cent., T-strain over 60 per cent.; Trichomonas 15 per cent.; Candida possibly over 5 per cent. Truly non-specific urethritis may account for 25 to 30 per cent. of cases."}
{"id": "PMID:1095152", "title": "An additional silver impregnation method for demonstration of degenerating nerve cells and processes in the central nervous system.", "content": "A new silver impregnation technique for frozen and paraffin sections of formalin-fixed nervous tissue has been devised by modifying the Fink-Heimer14 and De Olmos-Ingram6 methods. The modified versions have been combined into a single staining procedure. Cobalt or lead nitrate are substituted for uranyl nitrate. Silver nitrate in the impregnating solution is replaced by silver lactate or protargol. This procedure results in a preferential impregnation of degenerating perikarya, dendrites, axons and synaptic endings. It affords a more complete impregnation of 'preterminal' axonal arborizations than previous methods. The method is effective for the demonstration of degenerating terminals in the rat cerebellar cortex. Emphasis has been laid on the usefulness of the method for the study of drug-induced CNS lesions.", "contents": "An additional silver impregnation method for demonstration of degenerating nerve cells and processes in the central nervous system. A new silver impregnation technique for frozen and paraffin sections of formalin-fixed nervous tissue has been devised by modifying the Fink-Heimer14 and De Olmos-Ingram6 methods. The modified versions have been combined into a single staining procedure. Cobalt or lead nitrate are substituted for uranyl nitrate. Silver nitrate in the impregnating solution is replaced by silver lactate or protargol. This procedure results in a preferential impregnation of degenerating perikarya, dendrites, axons and synaptic endings. It affords a more complete impregnation of 'preterminal' axonal arborizations than previous methods. The method is effective for the demonstration of degenerating terminals in the rat cerebellar cortex. Emphasis has been laid on the usefulness of the method for the study of drug-induced CNS lesions."}
{"id": "PMID:1095159", "title": "A method for prevention of cardiovascular reactions to laryngoscopy and intubation.", "content": "A technique of nebulizing lidocaine in the upper airways was developed and its effectiveness in preventing cardiovascular responses to laryngoscopy and intubation was documented. In ten pre-treated patients the average percentage-rise in systolic blood pressure was 10.3 per cent and there were no arrhythmias. The pulse rate increased by 16.8 per cent. In ten control patients, the systolic blood pressure increased by 56 per cent and the incidence of arrhythmias was 40 per cent. The pulse rate increased by 38.8 per cent. The significance and application of these results is discussed and compared to other available methods. The opinion is expressed that the effect of the aerosol, especially as it relates to the suppression of arrhythmias, is partly due to systemic absorption of lidocaine.", "contents": "A method for prevention of cardiovascular reactions to laryngoscopy and intubation. A technique of nebulizing lidocaine in the upper airways was developed and its effectiveness in preventing cardiovascular responses to laryngoscopy and intubation was documented. In ten pre-treated patients the average percentage-rise in systolic blood pressure was 10.3 per cent and there were no arrhythmias. The pulse rate increased by 16.8 per cent. In ten control patients, the systolic blood pressure increased by 56 per cent and the incidence of arrhythmias was 40 per cent. The pulse rate increased by 38.8 per cent. The significance and application of these results is discussed and compared to other available methods. The opinion is expressed that the effect of the aerosol, especially as it relates to the suppression of arrhythmias, is partly due to systemic absorption of lidocaine."}
{"id": "PMID:1095160", "title": "The effects of continuous positive pressure ventilation on pulmonary oedema, gas exchange and lung mechanics.", "content": "The effect of continuous positive pressure ventilation was studied in 23 dogs in whom pulmonary oedema was induced by rapid intravenous infusion of dextran 40. CPPV slowed but did not prevent the formation of oedema. CPPV prevented or reversed the formation of liver-like areas in the dependent lobes, maintaining the oedematous lung compliant and aerated. This led to a reduction of shunt and an increase in PaO2. The use of CPPV decreased cardiac output and body oxygen consumption. Mixed venous oxygen tension was higher in dogs ventilated by CPPV and these dogs tolerated fulminant frothy oedema without distress.", "contents": "The effects of continuous positive pressure ventilation on pulmonary oedema, gas exchange and lung mechanics. The effect of continuous positive pressure ventilation was studied in 23 dogs in whom pulmonary oedema was induced by rapid intravenous infusion of dextran 40. CPPV slowed but did not prevent the formation of oedema. CPPV prevented or reversed the formation of liver-like areas in the dependent lobes, maintaining the oedematous lung compliant and aerated. This led to a reduction of shunt and an increase in PaO2. The use of CPPV decreased cardiac output and body oxygen consumption. Mixed venous oxygen tension was higher in dogs ventilated by CPPV and these dogs tolerated fulminant frothy oedema without distress."}
{"id": "PMID:1095162", "title": "Serum free fatty acid and blood sugar levels in children under halothane, thiopentone and ketamine anaesthesia (comparative study).", "content": "Serum free fatty acid and blood glucose levels were determined in 30 children under halothane, thiopentone and ketamine anaesthesia. Blood sugar is increased above baseline values by halothane 54.75 per cent (+++), thiopentone 71.7 per cent (++++) and ketamine 12.2 per cent (+) anaesthesia. Serum free fatty acid was increased during halothane 58.7 per cent (+++) and thiopentone 34.4 per cent (++) anaesthesia, but was decreased with ketamine anaesthesia 13.4 per cent (-). It is assumed that several factors contribute to these changes and are related either to the sympathetic nervous system or basal metabolism and to the specific actions of each drug.", "contents": "Serum free fatty acid and blood sugar levels in children under halothane, thiopentone and ketamine anaesthesia (comparative study). Serum free fatty acid and blood glucose levels were determined in 30 children under halothane, thiopentone and ketamine anaesthesia. Blood sugar is increased above baseline values by halothane 54.75 per cent (+++), thiopentone 71.7 per cent (++++) and ketamine 12.2 per cent (+) anaesthesia. Serum free fatty acid was increased during halothane 58.7 per cent (+++) and thiopentone 34.4 per cent (++) anaesthesia, but was decreased with ketamine anaesthesia 13.4 per cent (-). It is assumed that several factors contribute to these changes and are related either to the sympathetic nervous system or basal metabolism and to the specific actions of each drug."}
{"id": "PMID:1095163", "title": "Post-operative epidural analgesia: effects on lung volumes.", "content": "A study was undertaken to assess the role of post-operative pain in reducing Functional Residual Capacity (FRC) and Vital Capacity (VC). The efficacy of post-operative epidural analgesia in reversing these changes was measured in eight subjects after upper abdominal operations. With pain, FRC and VC were 78 per cent and 37 per cent of control respectively. Following epidural analgesia, the values were 84 per cent and 55 per cent. These figures indicate the pain component in the post-operative reduction of these two lung capacities. This partial restoration may be of value in the prevention of post-operative pulmonary complications by avoiding closure of small airways during tidal breathing and by increasing the effectiveness of deep breathing and coughing in the removal of secretions and the reversal of atelectasis.", "contents": "Post-operative epidural analgesia: effects on lung volumes. A study was undertaken to assess the role of post-operative pain in reducing Functional Residual Capacity (FRC) and Vital Capacity (VC). The efficacy of post-operative epidural analgesia in reversing these changes was measured in eight subjects after upper abdominal operations. With pain, FRC and VC were 78 per cent and 37 per cent of control respectively. Following epidural analgesia, the values were 84 per cent and 55 per cent. These figures indicate the pain component in the post-operative reduction of these two lung capacities. This partial restoration may be of value in the prevention of post-operative pulmonary complications by avoiding closure of small airways during tidal breathing and by increasing the effectiveness of deep breathing and coughing in the removal of secretions and the reversal of atelectasis."}
{"id": "PMID:1095164", "title": "Poly IC therapy in aleutian disease of mink.", "content": "Twenty-four virgin female aleutian mink were infected with aleutian disease agent and after 24 hours, 12 of these were treated with a course of polyinosinic acid-polycytidilic acid (Poly IC) injections. After six weeks the gammaglobulin level was significantly lower in the treated group but at 12 weeks this difference was no longer present. Four of the treated mink had normal target organ histology when killed at 20 weeks. The untreated group all showed moderate to marked changes but this difference was not statistically significant. There was a marked increase in the reactive lymphocyte blastogenesis index during the first weeks of infection and the phytohaemagglutinin response was seen to fall progressively. The antiglobulin reaction usually became positive after infection but neither antinuclear nor antierythrocyte antibodies were found. Precipitating antibodies to several polynucleotides were frequently present and were unrelated to infection or to Poly IC treatment.", "contents": "Poly IC therapy in aleutian disease of mink. Twenty-four virgin female aleutian mink were infected with aleutian disease agent and after 24 hours, 12 of these were treated with a course of polyinosinic acid-polycytidilic acid (Poly IC) injections. After six weeks the gammaglobulin level was significantly lower in the treated group but at 12 weeks this difference was no longer present. Four of the treated mink had normal target organ histology when killed at 20 weeks. The untreated group all showed moderate to marked changes but this difference was not statistically significant. There was a marked increase in the reactive lymphocyte blastogenesis index during the first weeks of infection and the phytohaemagglutinin response was seen to fall progressively. The antiglobulin reaction usually became positive after infection but neither antinuclear nor antierythrocyte antibodies were found. Precipitating antibodies to several polynucleotides were frequently present and were unrelated to infection or to Poly IC treatment."}
{"id": "PMID:1095169", "title": "Evacuated blood-collection tubes--the backflow hazard.", "content": "Five cases of nosocomial infection caused by Serratia marcescens were traced to backflow of blood from nonsterile evacuated blood-collection tubes. The mechanism of backflow was investigated theoretically and the conditions were determined under which backflow can occur. The theory was confirmed by experiments conducted on a simulated venous system and by measurements of the venous pressure in the brachial vein of a patient during application and removal of a tourniquet. Various possible solutions to the backflow hazard include strict adherence to correct venipuncture technique, sterilization of all blood-collection tubes, improvement of the vacuum in the tubes and incorporation of a check valve into the system.", "contents": "Evacuated blood-collection tubes--the backflow hazard. Five cases of nosocomial infection caused by Serratia marcescens were traced to backflow of blood from nonsterile evacuated blood-collection tubes. The mechanism of backflow was investigated theoretically and the conditions were determined under which backflow can occur. The theory was confirmed by experiments conducted on a simulated venous system and by measurements of the venous pressure in the brachial vein of a patient during application and removal of a tourniquet. Various possible solutions to the backflow hazard include strict adherence to correct venipuncture technique, sterilization of all blood-collection tubes, improvement of the vacuum in the tubes and incorporation of a check valve into the system."}
{"id": "PMID:1095170", "title": "Beclomethasone dipropionate in asthma.", "content": "Beclomethasone dipropionate aerosol therapy can replace or diminish systemic corticosteroid therapy in the majority of asthmatics. In a clinical trial of 41 patients with perennial asthma, the 10 who had not required long-term corticosteroid therapy improved symptomatically and in pulmonary function. Of the 31 who had required prolonged systemic corticosteroid therapy 12 were able to discontinue oral prednisone therapy, 15 were able to decrease the maintenance dose of prednisone and only 4 were unable to decrease the dose; all maintained satisfactory lung function and some showed improvement. Discontinuation of systemic corticosteroid therapy was accomplished more readily in patients whose daily maintenance dose was less than 15 mg and who had been taking the drug for less than 3 years. Side effects consisted of a \"dry throat\" in seven patients, two of whom had throat infections with Candida albicans. Recurrence of rhinitis after discontinuation or reduction of systemic corticosteroid therapy was noted in 11 patients.", "contents": "Beclomethasone dipropionate in asthma. Beclomethasone dipropionate aerosol therapy can replace or diminish systemic corticosteroid therapy in the majority of asthmatics. In a clinical trial of 41 patients with perennial asthma, the 10 who had not required long-term corticosteroid therapy improved symptomatically and in pulmonary function. Of the 31 who had required prolonged systemic corticosteroid therapy 12 were able to discontinue oral prednisone therapy, 15 were able to decrease the maintenance dose of prednisone and only 4 were unable to decrease the dose; all maintained satisfactory lung function and some showed improvement. Discontinuation of systemic corticosteroid therapy was accomplished more readily in patients whose daily maintenance dose was less than 15 mg and who had been taking the drug for less than 3 years. Side effects consisted of a \"dry throat\" in seven patients, two of whom had throat infections with Candida albicans. Recurrence of rhinitis after discontinuation or reduction of systemic corticosteroid therapy was noted in 11 patients."}
{"id": "PMID:1095171", "title": "The permanent Tenckhoff catheter for chronic peritoneal dialysis.", "content": "Over a 3 1/2-year period the permanent Tenckhoff catheter was used in 66 patients (32 men and 34 women) maintained on chronic peritoneal dialysis for periods from 2 1/2 to 36 1/2 months; 57 patients had dialysis in hospital for 20 to 24 hours twice a week and the other 9 had dialysis at home for 10 to 12 hours four times a week. While the Tenckhoff catheter was in place 14 patients received a renal transplant; for 13 who required peritoneal dialysis during the post-transplant phase the Tenckhoff catheter was used. In nine patients abdominal surgery did not interfere with the continuation of peritoneal dialysis via the Tenckhoff catheter. From a total of 5067 dialyses 40 positive cultures were reported (0.8%). Peritonitis was clinically evident on only 14 occasions (0.28%). Permanent catheter obstruction developed in 16 patients, in 11 of whom it was related to peritonitis. With the introduction of the permanent Tenckhoff catheter long-term peritoneal dialysis has become a simple, safe and painless procedure, suitable for virtually all patients who require maintenance dialysis.", "contents": "The permanent Tenckhoff catheter for chronic peritoneal dialysis. Over a 3 1/2-year period the permanent Tenckhoff catheter was used in 66 patients (32 men and 34 women) maintained on chronic peritoneal dialysis for periods from 2 1/2 to 36 1/2 months; 57 patients had dialysis in hospital for 20 to 24 hours twice a week and the other 9 had dialysis at home for 10 to 12 hours four times a week. While the Tenckhoff catheter was in place 14 patients received a renal transplant; for 13 who required peritoneal dialysis during the post-transplant phase the Tenckhoff catheter was used. In nine patients abdominal surgery did not interfere with the continuation of peritoneal dialysis via the Tenckhoff catheter. From a total of 5067 dialyses 40 positive cultures were reported (0.8%). Peritonitis was clinically evident on only 14 occasions (0.28%). Permanent catheter obstruction developed in 16 patients, in 11 of whom it was related to peritonitis. With the introduction of the permanent Tenckhoff catheter long-term peritoneal dialysis has become a simple, safe and painless procedure, suitable for virtually all patients who require maintenance dialysis."}
{"id": "PMID:1095204", "title": "Compartments and cell flows within the mouse haemopoietic system. II. Estimated rates of interchange.", "content": "Part-body irradiated CBA mice were injected with CBA-T6 bone marrow. In this way a predominantly donor population was established in the femora while the marrow of the humeri remained largely (average 94%) of host origin. In animals examined cytologically up to 2 years later, no tendency was observed for the proportion of donor cells in the humeri to increase. Splenectomy had no effect on this. When femoral bone marrow from the experimental mice was injected into lethally (whole-body) irradiated recipients, cells originating from the primary host repopulated the lymph nodes to a disproportionate extent. Equilibration between the cell populations of femora and humeri occurred after re-exposure to 600 rad whole-body irradiation, but not after 100 rad or 350 rad; thus, regeneration of damaged bone marrow involved a significant contribution from extrinsic stem cells only after the highest dose of radiation. The data are compatible with an inflow of at most ten effective stem cells per humerus per day from the blood, and suggest a much lower figure. This means that few if any of the stem cells of peripheral blood enter the bone marrow and found haemopoietic clones. Evidence is adduced for the existence of a proliferating lymphoid sub-population in the bone marrow, contributing some 5-10% of the observed mitoses. The mitotic cells in the lymph nodes are replaced from marrow-derived progenitors at an estimated rate of 4-5%/day. The relevant data for the thymus are more variable, but suggest an average figure of 8-11%/day. Earlier data from mouse parabionts suggest a lower rate of inflow to the thymus.", "contents": "Compartments and cell flows within the mouse haemopoietic system. II. Estimated rates of interchange. Part-body irradiated CBA mice were injected with CBA-T6 bone marrow. In this way a predominantly donor population was established in the femora while the marrow of the humeri remained largely (average 94%) of host origin. In animals examined cytologically up to 2 years later, no tendency was observed for the proportion of donor cells in the humeri to increase. Splenectomy had no effect on this. When femoral bone marrow from the experimental mice was injected into lethally (whole-body) irradiated recipients, cells originating from the primary host repopulated the lymph nodes to a disproportionate extent. Equilibration between the cell populations of femora and humeri occurred after re-exposure to 600 rad whole-body irradiation, but not after 100 rad or 350 rad; thus, regeneration of damaged bone marrow involved a significant contribution from extrinsic stem cells only after the highest dose of radiation. The data are compatible with an inflow of at most ten effective stem cells per humerus per day from the blood, and suggest a much lower figure. This means that few if any of the stem cells of peripheral blood enter the bone marrow and found haemopoietic clones. Evidence is adduced for the existence of a proliferating lymphoid sub-population in the bone marrow, contributing some 5-10% of the observed mitoses. The mitotic cells in the lymph nodes are replaced from marrow-derived progenitors at an estimated rate of 4-5%/day. The relevant data for the thymus are more variable, but suggest an average figure of 8-11%/day. Earlier data from mouse parabionts suggest a lower rate of inflow to the thymus."}
{"id": "PMID:1095210", "title": "Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda.", "content": "Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational symmetry are present in each of the sites. Superimposed on each operator is an E. coli RNA polymerase recognition site (promoter). Similarities in the sequences of the two lambda promoters, a lac promoter, and an E. coli RNA polymerase recognition site in SV40 DNA are noted.", "contents": "Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda. Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational symmetry are present in each of the sites. Superimposed on each operator is an E. coli RNA polymerase recognition site (promoter). Similarities in the sequences of the two lambda promoters, a lac promoter, and an E. coli RNA polymerase recognition site in SV40 DNA are noted."}
{"id": "PMID:1095238", "title": "Effect of 5-bromodeoxyuridine on the transcriptional properties of the genome in WI-38 human diploid fibroblasts.", "content": "Growth of WI-38 diploid fibroblasts in a medium containing 5-bromodeoxyuridine (BrdU) resulted in an increased GMP and a decreased AMP incorporation into the RNA synthesised in vitro on a chromatin template. This effect was similar to that previously reported using 3T6 mouse fibroblasts-1. Substitution of thymidine by BrdU in DNA, also altered the characteristics of the DNA template itself, since the increased incorporation of guanine and decreased incorporation of adenine into RNA were evident also when purified, isolated DNA was used as template. The extent of replacement of AMP by GMP was proportional to the extent of replacement of thymidine by BrdU. Although there are variations in the base composition of RNA transcribed from BrdU-containing DNA templates, there are no significant difference in overall template activity or in the number of available chromatin binding sites for E. coli RNA polymerase. Confluent monolayers of BrdU-treated WI-38 fibroblasts are still able to respond with cell proliferation to a change of medium, as evidenced by an increased incorporation of (-3H)thymidine and an increase in chromatin template activity. The length of the prereplicative phase is similar in both BrdU-treated and untreated cells, although the magnitude of the increase of (-3H)thymidine incorporation is reduced by approximately 30% after BrdU treatment. The increase in chromatin template activity is associated with an increase in the number of chromatin binding sites for E. COLI RNA polymerase, suggesting that the presence of BrdU does not interfere with the availability of initiation sites or alter the actual rate of transcription.", "contents": "Effect of 5-bromodeoxyuridine on the transcriptional properties of the genome in WI-38 human diploid fibroblasts. Growth of WI-38 diploid fibroblasts in a medium containing 5-bromodeoxyuridine (BrdU) resulted in an increased GMP and a decreased AMP incorporation into the RNA synthesised in vitro on a chromatin template. This effect was similar to that previously reported using 3T6 mouse fibroblasts-1. Substitution of thymidine by BrdU in DNA, also altered the characteristics of the DNA template itself, since the increased incorporation of guanine and decreased incorporation of adenine into RNA were evident also when purified, isolated DNA was used as template. The extent of replacement of AMP by GMP was proportional to the extent of replacement of thymidine by BrdU. Although there are variations in the base composition of RNA transcribed from BrdU-containing DNA templates, there are no significant difference in overall template activity or in the number of available chromatin binding sites for E. coli RNA polymerase. Confluent monolayers of BrdU-treated WI-38 fibroblasts are still able to respond with cell proliferation to a change of medium, as evidenced by an increased incorporation of (-3H)thymidine and an increase in chromatin template activity. The length of the prereplicative phase is similar in both BrdU-treated and untreated cells, although the magnitude of the increase of (-3H)thymidine incorporation is reduced by approximately 30% after BrdU treatment. The increase in chromatin template activity is associated with an increase in the number of chromatin binding sites for E. COLI RNA polymerase, suggesting that the presence of BrdU does not interfere with the availability of initiation sites or alter the actual rate of transcription."}
{"id": "PMID:1095242", "title": "The interaction of natural tetra-azacyclopentazulene dyes with DNA and their effects on the DNA and RNA polymerase reactions.", "content": "The interaction of two natural tetra-azacyclopentazulene dyes with native calf thymus DNA was studied by means of microcalorimetric, viscosimetric, and spectroscopic measurements. The results are consistent with the hypothesis of an intercalative-binding. However, comparison of calorimetric studies shows that the changes in enthalpy associated with the interaction of these compounds with DNA are, in absolute value, significantly lower than those found with known intercalating agnets (daunomycin, ethidium bromide).  The influence of these dyes on the template capacity of DNA in the in vitro synthesis of nucleic acids was also determined.  Under the conditions used, these compounds selectively inhibited DNA synthesis.  No appreciable inhibitory effect upon E. coli RNA polymerase was observed.  Both compounds had greater inhibitory effect on rat liver high molecular weight DNA polymerase than E. coli DNA polymerase I.  Zoanthoxanthin was a more effective inhibitor than 3-norzoanthoxanthin.", "contents": "The interaction of natural tetra-azacyclopentazulene dyes with DNA and their effects on the DNA and RNA polymerase reactions. The interaction of two natural tetra-azacyclopentazulene dyes with native calf thymus DNA was studied by means of microcalorimetric, viscosimetric, and spectroscopic measurements. The results are consistent with the hypothesis of an intercalative-binding. However, comparison of calorimetric studies shows that the changes in enthalpy associated with the interaction of these compounds with DNA are, in absolute value, significantly lower than those found with known intercalating agnets (daunomycin, ethidium bromide).  The influence of these dyes on the template capacity of DNA in the in vitro synthesis of nucleic acids was also determined.  Under the conditions used, these compounds selectively inhibited DNA synthesis.  No appreciable inhibitory effect upon E. coli RNA polymerase was observed.  Both compounds had greater inhibitory effect on rat liver high molecular weight DNA polymerase than E. coli DNA polymerase I.  Zoanthoxanthin was a more effective inhibitor than 3-norzoanthoxanthin."}
{"id": "PMID:1095245", "title": "The effect of intracoronary injection of contrast medium upon coronary blood flow.", "content": "The changes in coronary blood flow in response to intracoronary injection of 3 ml of 76% Renografin were studied in 47 patients using the thermodilution technique for continuous measurement of coronary sinus blood flow. Within seconds after left coronary injection, an increase in coronary sinus flow began which peaked at an average of 53% above control in 5-10 seconds. There was a corresponding decrease in coronary resistance. Flow returned to control level in almost all patients within one minute of injection. Twenty-four of 35 patients had no change in coronary sinus flow in response to right coronary injection. This can be explained by the fact that most of the venous flow from the right coronary artery returns in such a way that it cannot be measured by the coronary sinus catheter. Of the eleven patients who did show an increase, seven had angiographically documented right to left collaterals, suggesting that the increase in flow was the result of vasodilatation of the left coronary bed by contrast arriving via the right to left collaterals. The percent changes in flow and resistance in response to left coronary injection were isgnificantly greater in the 13 normals than in the 34 with obstructive disease of the left coronary artery (P lessthan 0.01). Flow rose 70 plus or minus 27% (mean plus or minus standard deviation) in the normals versus 46 plus or minus 25% in the patients with coronary artery disease, while resistance fell 44 plus or minus 9% versus 33 plus or minus 11%. The differences, however, were not sufficient for these changes to be of value in the assessment of the degree of impairment of the coronary arterial bed in the individual patient.", "contents": "The effect of intracoronary injection of contrast medium upon coronary blood flow. The changes in coronary blood flow in response to intracoronary injection of 3 ml of 76% Renografin were studied in 47 patients using the thermodilution technique for continuous measurement of coronary sinus blood flow. Within seconds after left coronary injection, an increase in coronary sinus flow began which peaked at an average of 53% above control in 5-10 seconds. There was a corresponding decrease in coronary resistance. Flow returned to control level in almost all patients within one minute of injection. Twenty-four of 35 patients had no change in coronary sinus flow in response to right coronary injection. This can be explained by the fact that most of the venous flow from the right coronary artery returns in such a way that it cannot be measured by the coronary sinus catheter. Of the eleven patients who did show an increase, seven had angiographically documented right to left collaterals, suggesting that the increase in flow was the result of vasodilatation of the left coronary bed by contrast arriving via the right to left collaterals. The percent changes in flow and resistance in response to left coronary injection were isgnificantly greater in the 13 normals than in the 34 with obstructive disease of the left coronary artery (P lessthan 0.01). Flow rose 70 plus or minus 27% (mean plus or minus standard deviation) in the normals versus 46 plus or minus 25% in the patients with coronary artery disease, while resistance fell 44 plus or minus 9% versus 33 plus or minus 11%. The differences, however, were not sufficient for these changes to be of value in the assessment of the degree of impairment of the coronary arterial bed in the individual patient."}
{"id": "PMID:1095246", "title": "Treatment of nasal polyps with intranasal beclomethasone dipropionate aerosol.", "content": "In a double-blind trial thirty-five patients with moderately-severe nasal polyposis were treated with intranasal beclomethasone dipropionate aerosol for 3 weeks. The dose given (400 mug/day) had only local effect on the symptoms. Judged by diary card scores the nasal symptoms were reduced to 52% of the pre-trial level for the whole group. Corrected for the placebo effect the percentage was 68. The reduction of symptoms was equally apportioned to the three symptoms, sneezing, nasal secretion and blockage. The treatment was tolerated well, and it is concluded that intranasal treatment with beclomethasone dipropionate aerosol offers most patients with nasal polyps a good response without any risk of systemic steroid side-effects.", "contents": "Treatment of nasal polyps with intranasal beclomethasone dipropionate aerosol. In a double-blind trial thirty-five patients with moderately-severe nasal polyposis were treated with intranasal beclomethasone dipropionate aerosol for 3 weeks. The dose given (400 mug/day) had only local effect on the symptoms. Judged by diary card scores the nasal symptoms were reduced to 52% of the pre-trial level for the whole group. Corrected for the placebo effect the percentage was 68. The reduction of symptoms was equally apportioned to the three symptoms, sneezing, nasal secretion and blockage. The treatment was tolerated well, and it is concluded that intranasal treatment with beclomethasone dipropionate aerosol offers most patients with nasal polyps a good response without any risk of systemic steroid side-effects."}
{"id": "PMID:1095247", "title": "Incidence of serum anti-DNA precipitins in patients with systemic lupus erythematosus by counterimmunoelectrophoresis.", "content": "The technique of counterimmunoelectrophoresis (CIE) has been adapted for detection of serum precipitins to calf thymus (CT) DNA in patients with SLE, discoid LE, miscellaneous connective tissue and infectious diseases, and control populations. Of seventy-eight LE patients, 58% demonstrated anti-ss DNA precipitins, and 20% exhibited anti-ds DNA precipitins. Good correlation was noted between the presence of ss DNA precipitins and ss DNA binding values determined by the more sensitive ammonium sulphate precipitation assay. Depressed total serum haemolytic complement activity in CH50 mu/ml was noted in 64% of sera exhibiting ss DNA precipitins and 38% of those with negative ss DNA precipitins. There was a strong association, however, between ds DNA precipitins and depressed serum complement levels. Although less sensitive than primary binding assays, CIE can be used as a rapid and simple screening test for detection of circulating anti-native and denatured CT DNA precipitins. CT DNA serum precipitins are present in a significantly higher percentage of SLE patients when compared with other disease states and normal control populations.", "contents": "Incidence of serum anti-DNA precipitins in patients with systemic lupus erythematosus by counterimmunoelectrophoresis. The technique of counterimmunoelectrophoresis (CIE) has been adapted for detection of serum precipitins to calf thymus (CT) DNA in patients with SLE, discoid LE, miscellaneous connective tissue and infectious diseases, and control populations. Of seventy-eight LE patients, 58% demonstrated anti-ss DNA precipitins, and 20% exhibited anti-ds DNA precipitins. Good correlation was noted between the presence of ss DNA precipitins and ss DNA binding values determined by the more sensitive ammonium sulphate precipitation assay. Depressed total serum haemolytic complement activity in CH50 mu/ml was noted in 64% of sera exhibiting ss DNA precipitins and 38% of those with negative ss DNA precipitins. There was a strong association, however, between ds DNA precipitins and depressed serum complement levels. Although less sensitive than primary binding assays, CIE can be used as a rapid and simple screening test for detection of circulating anti-native and denatured CT DNA precipitins. CT DNA serum precipitins are present in a significantly higher percentage of SLE patients when compared with other disease states and normal control populations."}
{"id": "PMID:1095248", "title": "Investigations of allergic status and blood counts in Chinese patients receiving daily or intermittent rifampicin in Hong Kong.", "content": "In a controlled trial in Hong Bong, 575 Chinese patients with pulmonary tuberculosis whose treatment with first-line regimens had failed were allocated at random to the following retreatment regimens of chemotherapy. (1) Rifampicin plus ethambutol daily (ER7). (2) Rifampicin plus ethambutol twice a week (ER2). (3) Rifampicin plus ethambutol once a week (ER1). (4) Rifampicin plus ethambutol daily for 2 months and then once a week (ER7ER1). (5) Ethionamide plus pyrazinamide plus cycloserine daily for 6 months and then ethionamide plus pyrazinamide daily (EtZC), as a control regimen. Answers to a questionnaire on allergic disease, the results of prick tests with standard allergens, ABO blood grouping, size of tuberculin response during chemotherapy, and a rifampicin patch test showed no associations with the occurrence of adverse reactions to daily or intermittent rifampicin. Mantoux testing during chemotherapy provided no evidence of an immunosuppressive effect of rifampicin. Mean platelet counts at 12 months were significantly lower than those at 3 months on the two once-weekly regimens (ER1, ER7ER1) and on the control regimen (EtZC), although still within normal limits. At 3 months, but not at 12 months, mean platelet counts on the two once-weekly regimens were significantly lower 6 hr after a dose of the regimen than they were before the dose.", "contents": "Investigations of allergic status and blood counts in Chinese patients receiving daily or intermittent rifampicin in Hong Kong. In a controlled trial in Hong Bong, 575 Chinese patients with pulmonary tuberculosis whose treatment with first-line regimens had failed were allocated at random to the following retreatment regimens of chemotherapy. (1) Rifampicin plus ethambutol daily (ER7). (2) Rifampicin plus ethambutol twice a week (ER2). (3) Rifampicin plus ethambutol once a week (ER1). (4) Rifampicin plus ethambutol daily for 2 months and then once a week (ER7ER1). (5) Ethionamide plus pyrazinamide plus cycloserine daily for 6 months and then ethionamide plus pyrazinamide daily (EtZC), as a control regimen. Answers to a questionnaire on allergic disease, the results of prick tests with standard allergens, ABO blood grouping, size of tuberculin response during chemotherapy, and a rifampicin patch test showed no associations with the occurrence of adverse reactions to daily or intermittent rifampicin. Mantoux testing during chemotherapy provided no evidence of an immunosuppressive effect of rifampicin. Mean platelet counts at 12 months were significantly lower than those at 3 months on the two once-weekly regimens (ER1, ER7ER1) and on the control regimen (EtZC), although still within normal limits. At 3 months, but not at 12 months, mean platelet counts on the two once-weekly regimens were significantly lower 6 hr after a dose of the regimen than they were before the dose."}
{"id": "PMID:1095261", "title": "A computer-oriented linkage analysis scheme.", "content": "A comprehensive, computer-oriented scheme for genetic linkage analysis is described. It computes the likelihood of a complex pedigree allowing for many non-standard features including multiple alleles (up to four per locus), multiple mates, more than one mating in the eldest generation, consanguinity, missing or partially tested persons, and different recombination fractions between males and females. The program works by computing weighted likelihoods in a vertical, descending fashion. First, the set of possible genotypes for the members of the eldest generation is determined and prior probabilities assigned to these states of nature (based on gene frequency calculations). Phenotypic information is used to modify these probabilities in the standard manner. Then, for a particular set of genotypes, the conditional likelihood of the remainder of the pedigree is computed. This calculation may itself be many-branched and therefore a computer is necessary to keep track of the stages of the process. When the youngest generation has been taken into account, a final likelihood is determined, reflecting all information in the pedigree. The current version of the program successfully calculates the exact likelihood of a complex pedigree which was designed to exploit the non-standard features of the program. It has also computed likelihoods for a published pedigree, and the results are essentially equal to those obtained by the other authors using a previously available computer scheme for linkage analysis.", "contents": "A computer-oriented linkage analysis scheme. A comprehensive, computer-oriented scheme for genetic linkage analysis is described. It computes the likelihood of a complex pedigree allowing for many non-standard features including multiple alleles (up to four per locus), multiple mates, more than one mating in the eldest generation, consanguinity, missing or partially tested persons, and different recombination fractions between males and females. The program works by computing weighted likelihoods in a vertical, descending fashion. First, the set of possible genotypes for the members of the eldest generation is determined and prior probabilities assigned to these states of nature (based on gene frequency calculations). Phenotypic information is used to modify these probabilities in the standard manner. Then, for a particular set of genotypes, the conditional likelihood of the remainder of the pedigree is computed. This calculation may itself be many-branched and therefore a computer is necessary to keep track of the stages of the process. When the youngest generation has been taken into account, a final likelihood is determined, reflecting all information in the pedigree. The current version of the program successfully calculates the exact likelihood of a complex pedigree which was designed to exploit the non-standard features of the program. It has also computed likelihoods for a published pedigree, and the results are essentially equal to those obtained by the other authors using a previously available computer scheme for linkage analysis."}
{"id": "PMID:1095266", "title": "Serum proteins as indicators of poor dietary intake in patients with chronic renal failure.", "content": "A study of serum proteins in patients on regular hemodialysis has shown that many have low serum transferrin levels but near normal serum albumin and normal or raised pre-albumin levels. Hemoglobin values were related to transferrin levels. Low transferrin levels also occurred in patients with advanced osteitis fibrosa. Deficient protein intake seems the likely explanation. Measurement of pre-albumin does not reflect low protein intake in chronic renal failure; reasons for this discussed.", "contents": "Serum proteins as indicators of poor dietary intake in patients with chronic renal failure. A study of serum proteins in patients on regular hemodialysis has shown that many have low serum transferrin levels but near normal serum albumin and normal or raised pre-albumin levels. Hemoglobin values were related to transferrin levels. Low transferrin levels also occurred in patients with advanced osteitis fibrosa. Deficient protein intake seems the likely explanation. Measurement of pre-albumin does not reflect low protein intake in chronic renal failure; reasons for this discussed."}
{"id": "PMID:1095262", "title": "Effect of equivalent antihypertensive doses of mefruside and cyclopenthiazide on serum electrolytes, uric acid and glucose tolerance in hypertensive patients.", "content": "1. A comparative cross-over trial of mefruside and cyclopenthiazide, each drug being given for 6 weeks, was conducted on thirty hypertensive patients with diabetes mellitus or impaired glucose tolerance. Other antihypertensive therapy, any antidiabetic therapy and potassium supplementation were kept constant throughout the trial. Dosages of mefruside and cyclopenthiazide were adjusted to give approximately equal blood pressure levels in the two drug periods. 2. There was no significant difference in the following parameters studied during the sixth week of each of the two periods of drug therapy: serum electrolytes, total CO2, chloride, urea, amylase, haemoglobin, erythrocyte sedimentation rate, platelet and white blood cell counts, lying and standing blood pressure and pulse rate, weight, fasting and 2-h glucose and insulin levels and five-value glucose and insulin curve areas, fasting calcium and phosphate. 3. Serum creatinine and uric acid showed a small but significant fall during mefruside therapy.", "contents": "Effect of equivalent antihypertensive doses of mefruside and cyclopenthiazide on serum electrolytes, uric acid and glucose tolerance in hypertensive patients. 1. A comparative cross-over trial of mefruside and cyclopenthiazide, each drug being given for 6 weeks, was conducted on thirty hypertensive patients with diabetes mellitus or impaired glucose tolerance. Other antihypertensive therapy, any antidiabetic therapy and potassium supplementation were kept constant throughout the trial. Dosages of mefruside and cyclopenthiazide were adjusted to give approximately equal blood pressure levels in the two drug periods. 2. There was no significant difference in the following parameters studied during the sixth week of each of the two periods of drug therapy: serum electrolytes, total CO2, chloride, urea, amylase, haemoglobin, erythrocyte sedimentation rate, platelet and white blood cell counts, lying and standing blood pressure and pulse rate, weight, fasting and 2-h glucose and insulin levels and five-value glucose and insulin curve areas, fasting calcium and phosphate. 3. Serum creatinine and uric acid showed a small but significant fall during mefruside therapy."}
{"id": "PMID:1095269", "title": "A comparative evaluation of three high efficiency disposable dialyzers.", "content": "Until recently all disposable dialyzers have had a poorer overall performance than the best non-disposable dialyzer, the Meltec multipoint. The three disposables considered here (Gambro Lundia Nova, Cordis HFAK 4 and Rhone Poulenc RP 5) all have clearance of small molecules close to or, for the HFAK 4, just above that of the multipoint though their middle molecular clearance is not quite as good as the multipoint. The HFAK 4 has a better basal ultranfiltration rate but a poorer maximum ultrafiltration capacity. The RP 5 has a higher residual blood volume than the multipoint. In other respects the dialyzers are almost interchangeable and are reasonable alternatives to the multipoint in those centers which can afford single use disposable dialyzers.", "contents": "A comparative evaluation of three high efficiency disposable dialyzers. Until recently all disposable dialyzers have had a poorer overall performance than the best non-disposable dialyzer, the Meltec multipoint. The three disposables considered here (Gambro Lundia Nova, Cordis HFAK 4 and Rhone Poulenc RP 5) all have clearance of small molecules close to or, for the HFAK 4, just above that of the multipoint though their middle molecular clearance is not quite as good as the multipoint. The HFAK 4 has a better basal ultranfiltration rate but a poorer maximum ultrafiltration capacity. The RP 5 has a higher residual blood volume than the multipoint. In other respects the dialyzers are almost interchangeable and are reasonable alternatives to the multipoint in those centers which can afford single use disposable dialyzers."}
{"id": "PMID:1095270", "title": "Rejection episodes and patient and graft survival after renal transplantation.", "content": "The occurrence of acute rejection episodes following renal transplantation was correlated with one-year patient mortality and graft failure rates in a series of 102 consecutive transplant recipients. Twenty-one patients had two or more rejection episodes in the first two months post transplantation; 17 of these expired or lost their kidney compared to only 5 of 28 patients with no rejection episodes in this time period. Similarly, of the 14 patients who developed a second rejection episode 2--6 months post transplantation, 8 died or lost the graft compared to only 2 of 21 patients who had no rejection episodes in this time period. Thus, the development of a second rejection episode early after transplantation necessitating treatment with high dose prednisone therapy carries an ominous prognosis and suggests that serious consideration must be given to graft removal and subsequent retransplantation.", "contents": "Rejection episodes and patient and graft survival after renal transplantation. The occurrence of acute rejection episodes following renal transplantation was correlated with one-year patient mortality and graft failure rates in a series of 102 consecutive transplant recipients. Twenty-one patients had two or more rejection episodes in the first two months post transplantation; 17 of these expired or lost their kidney compared to only 5 of 28 patients with no rejection episodes in this time period. Similarly, of the 14 patients who developed a second rejection episode 2--6 months post transplantation, 8 died or lost the graft compared to only 2 of 21 patients who had no rejection episodes in this time period. Thus, the development of a second rejection episode early after transplantation necessitating treatment with high dose prednisone therapy carries an ominous prognosis and suggests that serious consideration must be given to graft removal and subsequent retransplantation."}
{"id": "PMID:1095271", "title": "5-Fluorocytosine treatment of candidiasis on a patient receiving regular hemodialysis.", "content": "A patient developed a severe urinary infection with Candida albicans following a cadaveric renal transplant operation. It was necessary to remove the graft and return the patient to regular hemodialysis treatment. The fungi infection was successfully eradicated by a single oral dose of 5-Fluorocytosine (20 mg/kg) after each dialysis treatment. Pharmacokinetic studies revealed that the drug had a dialyzate clearance ratio of 0.66 when compared with creatinine; the drug was not metabolized and there was no other route of elimination.", "contents": "5-Fluorocytosine treatment of candidiasis on a patient receiving regular hemodialysis. A patient developed a severe urinary infection with Candida albicans following a cadaveric renal transplant operation. It was necessary to remove the graft and return the patient to regular hemodialysis treatment. The fungi infection was successfully eradicated by a single oral dose of 5-Fluorocytosine (20 mg/kg) after each dialysis treatment. Pharmacokinetic studies revealed that the drug had a dialyzate clearance ratio of 0.66 when compared with creatinine; the drug was not metabolized and there was no other route of elimination."}
{"id": "PMID:1095272", "title": "Amniocentesis.", "content": "The indications, technique, and potential complications of the now commonly used obstetric procedure, amniocentesis, have been reviewed. Although amniocentesis is considered by many a routine and a very safe procedure, there are potential complications, especially for the fetus, but for the mother as well. The frequency of these complications can be reduced by meticulous attention to technique and especially by preliminary placental localization. The latter is probably best accomplished by the ultrasound B scan. Because there are potential complications, the indications and timing of amniocentesis must always be carefully weighed before it is carried out.", "contents": "Amniocentesis. The indications, technique, and potential complications of the now commonly used obstetric procedure, amniocentesis, have been reviewed. Although amniocentesis is considered by many a routine and a very safe procedure, there are potential complications, especially for the fetus, but for the mother as well. The frequency of these complications can be reduced by meticulous attention to technique and especially by preliminary placental localization. The latter is probably best accomplished by the ultrasound B scan. Because there are potential complications, the indications and timing of amniocentesis must always be carefully weighed before it is carried out."}
{"id": "PMID:1095276", "title": "Tumors of the hand.", "content": "A review of 129 hand tumors suggests that diagnosis of a swelling in the hand is often uncertain because of the close proximity of many different tissues in a small volume. Histologic diagnosis is necessary to assure appropriate treatment; excisional biopsy should be done whenever possible; if the lesion is benign, treatment has been completed; in the event the lesion is malignant, further treatment will not have been materially compromised. The margin of normal tissue around malignant lesions often precludes treatment of such tumors of the hand by ray resection or wide local excision because adequate wide excision will frequently leave nothing functional. Attempts at wide local excision are ofter compromised by the surgeon's natural desire to preserve function; this frequently results in incomplete removal of the tumor. The function preserved will be of little benefit to a patient with widespread metastases.", "contents": "Tumors of the hand. A review of 129 hand tumors suggests that diagnosis of a swelling in the hand is often uncertain because of the close proximity of many different tissues in a small volume. Histologic diagnosis is necessary to assure appropriate treatment; excisional biopsy should be done whenever possible; if the lesion is benign, treatment has been completed; in the event the lesion is malignant, further treatment will not have been materially compromised. The margin of normal tissue around malignant lesions often precludes treatment of such tumors of the hand by ray resection or wide local excision because adequate wide excision will frequently leave nothing functional. Attempts at wide local excision are ofter compromised by the surgeon's natural desire to preserve function; this frequently results in incomplete removal of the tumor. The function preserved will be of little benefit to a patient with widespread metastases."}
{"id": "PMID:1095277", "title": "Somatomedin.", "content": "Somatomedin is a peptide component of serum which has been postulated to mediate the action of growth hormone on skeletal tissue. Direct effects on cartilage include stimulation of the synthesis of mucopolysacharide, protein, and nucleic acids. Insulin-like effects on non-skeletal tissues and cells have been described, and a relationship to NSILA-S and MSA has been suggested. The liver may be an important source. Non-specificity of bioassays is a problem. Growth hormone deficiency, malnutrition, therapy with corticosteroids or estrogens, and a type of dwarfism characterized by high serum growth hormone are associated with decreased somatomedin. An unexplained phenomenon is the normal somatomedin with low or undetectable growth hormone in certain cases of craniopharyngioma or other tumors involving the hypothalamus. Somatomedin is increased in acromegaly.", "contents": "Somatomedin. Somatomedin is a peptide component of serum which has been postulated to mediate the action of growth hormone on skeletal tissue. Direct effects on cartilage include stimulation of the synthesis of mucopolysacharide, protein, and nucleic acids. Insulin-like effects on non-skeletal tissues and cells have been described, and a relationship to NSILA-S and MSA has been suggested. The liver may be an important source. Non-specificity of bioassays is a problem. Growth hormone deficiency, malnutrition, therapy with corticosteroids or estrogens, and a type of dwarfism characterized by high serum growth hormone are associated with decreased somatomedin. An unexplained phenomenon is the normal somatomedin with low or undetectable growth hormone in certain cases of craniopharyngioma or other tumors involving the hypothalamus. Somatomedin is increased in acromegaly."}
{"id": "PMID:1095278", "title": "Immunology of cartilage in septic arthritis.", "content": "Septic arthritis in one knee joint was produced in 18 rabbits, the contralateral knee being left intact. Sections stained with fluorescein conjugated anti-IgG and with fluorescein conjugated anti-IgM showed layers of immunoglobulins on the articular cartilage in 16 out of 17 injected joints, and 10 out of 17 supposedly \"normal\" knee joints. Concurrent tests for circulating anti-staphylococcal alpha-lysin gave negative results. Cultures of synovial fluids were negative. These observations suggest that articular cartilage may act as an auto-antigen; immunological mechanisms involved may be due to the combined activity of both cell-bound and humoral antibodies. There are 2 components to a septic arthritis, the primary being a bacterial and the secondary immunologic in nature.", "contents": "Immunology of cartilage in septic arthritis. Septic arthritis in one knee joint was produced in 18 rabbits, the contralateral knee being left intact. Sections stained with fluorescein conjugated anti-IgG and with fluorescein conjugated anti-IgM showed layers of immunoglobulins on the articular cartilage in 16 out of 17 injected joints, and 10 out of 17 supposedly \"normal\" knee joints. Concurrent tests for circulating anti-staphylococcal alpha-lysin gave negative results. Cultures of synovial fluids were negative. These observations suggest that articular cartilage may act as an auto-antigen; immunological mechanisms involved may be due to the combined activity of both cell-bound and humoral antibodies. There are 2 components to a septic arthritis, the primary being a bacterial and the secondary immunologic in nature."}
{"id": "PMID:1095281", "title": "Morphine and phenytoin binding to plasma proteins in renal and hepatic failure.", "content": "The binding of morphine and phenytoin to plasma proteins was examined in healthy subjects and in patients with renal and hepatic failure. In the uremic patients without hepatic failure, morphine binding was dependent on the concentration of total serum proteins and albumin, but not the severity of renal failure as measured by creatinine clearance. Binding of phenytoin, however, was dependent on the degree of renal failure and albumin concentration, but not on total serum protein concentration. Renal transplant in 1 patient restored the binding of both drugs to a value within the normal range. The combination of hypoalbuminemia and hyperbilirubinemia resulted in the greatest impairment to binding for both drugs. It is concluded that patients with uremia, jaundice, hypoalbuminemia, particularly in combination, are sensitive to usual clinical doses of morphine, at least in part, because of decreased binding to plasma proteins.", "contents": "Morphine and phenytoin binding to plasma proteins in renal and hepatic failure. The binding of morphine and phenytoin to plasma proteins was examined in healthy subjects and in patients with renal and hepatic failure. In the uremic patients without hepatic failure, morphine binding was dependent on the concentration of total serum proteins and albumin, but not the severity of renal failure as measured by creatinine clearance. Binding of phenytoin, however, was dependent on the degree of renal failure and albumin concentration, but not on total serum protein concentration. Renal transplant in 1 patient restored the binding of both drugs to a value within the normal range. The combination of hypoalbuminemia and hyperbilirubinemia resulted in the greatest impairment to binding for both drugs. It is concluded that patients with uremia, jaundice, hypoalbuminemia, particularly in combination, are sensitive to usual clinical doses of morphine, at least in part, because of decreased binding to plasma proteins."}
{"id": "PMID:1095282", "title": "Comparative effects of tolamolol and propranolol on cardiac and peripheral circulatory function in patients with coronary artery disease.", "content": "In a clinical study comparing the cardiocirculatroy effects of intravenous tolamolol to those of propranolol, tolamolol, 16 mg, induced similar reduction in resting heart rate as 8 mg propranolol in 16 coronary patients. Tolamolol did not disturb cardiac pump performance and exerted less negative inotropic action than propranolol as assessed by mechanical contractility indices. Myocardial beta-one chronotropic and inotropic stimulation by exogenous epinephrine was blocked equally by tolamolol and propranolol. Tolamolol exerted less systemic vascular beta-two blockade than propranolol as assessed by the peripheral resistance and vasopressor responses to epinephrine infusion. Tolamolction than propranolol and is thereby suitable for careful extension of beta blockade therapy to certain patients with pulmonary  and ventricular dysfunction.", "contents": "Comparative effects of tolamolol and propranolol on cardiac and peripheral circulatory function in patients with coronary artery disease. In a clinical study comparing the cardiocirculatroy effects of intravenous tolamolol to those of propranolol, tolamolol, 16 mg, induced similar reduction in resting heart rate as 8 mg propranolol in 16 coronary patients. Tolamolol did not disturb cardiac pump performance and exerted less negative inotropic action than propranolol as assessed by mechanical contractility indices. Myocardial beta-one chronotropic and inotropic stimulation by exogenous epinephrine was blocked equally by tolamolol and propranolol. Tolamolol exerted less systemic vascular beta-two blockade than propranolol as assessed by the peripheral resistance and vasopressor responses to epinephrine infusion. Tolamolction than propranolol and is thereby suitable for careful extension of beta blockade therapy to certain patients with pulmonary  and ventricular dysfunction."}
{"id": "PMID:1095283", "title": "Fenmetozole in acute alcholol intoxication in man.", "content": "Forty healthy adult male volunteers were studied to determine the efficacy of fenmetozole to antagonize the effects of acute alcholol intoxication. Twenty subjects receive placebo and 20 fenmetozole in dosage of 100 mg and 200 mg in a double-blind paradigm. Pretreatment with fenmetozole failed to antogonize or attenuate cognitive, perceptual, motor and affective changes associated with acute alchol intoxication.", "contents": "Fenmetozole in acute alcholol intoxication in man. Forty healthy adult male volunteers were studied to determine the efficacy of fenmetozole to antagonize the effects of acute alcholol intoxication. Twenty subjects receive placebo and 20 fenmetozole in dosage of 100 mg and 200 mg in a double-blind paradigm. Pretreatment with fenmetozole failed to antogonize or attenuate cognitive, perceptual, motor and affective changes associated with acute alchol intoxication."}
{"id": "PMID:1095284", "title": "A review and discussion of goals in community dentistry.", "content": "The author discusses the need for clearly stated goals in community dentistry, the choice of goals, and the chances to achieve them. Goals in community health planning should be based on the values, needs and resources of the community in question. When analyzing goals a distinction should be made between dental health and dental care. Some goals for dental care, e.g. equity of access, may concern social justice but do not necessarily lead to better dental health. It is generally agreed that good dental health for a whole community cannot be achieved by restorative treatment alone; good dental health habits are considered to be a better way to promote dental health. Existing research indicates that there are chances to attain an acceptable level of dental health if the community effort is systematically planned and executed. There are indications that a reduction in the prevalence of dental disease can be brought about by new and effective means in the future. Long-term planning should be flexible enough to respond to new opportunities.", "contents": "A review and discussion of goals in community dentistry. The author discusses the need for clearly stated goals in community dentistry, the choice of goals, and the chances to achieve them. Goals in community health planning should be based on the values, needs and resources of the community in question. When analyzing goals a distinction should be made between dental health and dental care. Some goals for dental care, e.g. equity of access, may concern social justice but do not necessarily lead to better dental health. It is generally agreed that good dental health for a whole community cannot be achieved by restorative treatment alone; good dental health habits are considered to be a better way to promote dental health. Existing research indicates that there are chances to attain an acceptable level of dental health if the community effort is systematically planned and executed. There are indications that a reduction in the prevalence of dental disease can be brought about by new and effective means in the future. Long-term planning should be flexible enough to respond to new opportunities."}
{"id": "PMID:1095292", "title": "Cerebral Paragonimiasis.", "content": "The first case of cerebral paragonimiasis was reported by Otani in Japan in 1887. This was nine years after Kerbert's discovery of the fluke in the lungs of Bengal tigers and seven years after a human pulmonary infection by the fluke was demonstrated by Baelz and Manson. The first case was a 26-year-old man who had been suffering from cough and hemosputum for one year. The patient developed convulsive seizures with subsequent coma and died. The postmortem examination showed cystic lesions in the right frontal and occipital lobes. An adult fluke was found in the occipital lesion and another was seen in a gross specimen of normal brain tissue around the affected occipital lobe. Two years after Otani's discovery, at autopsy a 29-year-old man with a history of Jacksonian seizure was reported as having cerebral paragonimiasis. Some time later, however, it was confirmed that the case was actually cerebral schistosomiasis japonica.  Subsequently, cases of cerebral paragonimiasis were reported. However, the majority of these cases were not confirmed histologically. It was pointed out that some of these early cases were probably not Paragonimus infection. After World War II, reviews as well as case reports were published. Recently, investigations have been reported from Korea, with a clinicla study on 62 cases of cerebral paragonimiasis seen at the Neurology Department of the National Medical Center, Seoul, between 1958 and 1964. In 1971 Higashi described a statistical study on 105 cases of cerebral paragonimiasis that had been treated surgically in Japan.", "contents": "Cerebral Paragonimiasis. The first case of cerebral paragonimiasis was reported by Otani in Japan in 1887. This was nine years after Kerbert's discovery of the fluke in the lungs of Bengal tigers and seven years after a human pulmonary infection by the fluke was demonstrated by Baelz and Manson. The first case was a 26-year-old man who had been suffering from cough and hemosputum for one year. The patient developed convulsive seizures with subsequent coma and died. The postmortem examination showed cystic lesions in the right frontal and occipital lobes. An adult fluke was found in the occipital lesion and another was seen in a gross specimen of normal brain tissue around the affected occipital lobe. Two years after Otani's discovery, at autopsy a 29-year-old man with a history of Jacksonian seizure was reported as having cerebral paragonimiasis. Some time later, however, it was confirmed that the case was actually cerebral schistosomiasis japonica.  Subsequently, cases of cerebral paragonimiasis were reported. However, the majority of these cases were not confirmed histologically. It was pointed out that some of these early cases were probably not Paragonimus infection. After World War II, reviews as well as case reports were published. Recently, investigations have been reported from Korea, with a clinicla study on 62 cases of cerebral paragonimiasis seen at the Neurology Department of the National Medical Center, Seoul, between 1958 and 1964. In 1971 Higashi described a statistical study on 105 cases of cerebral paragonimiasis that had been treated surgically in Japan."}
{"id": "PMID:1095293", "title": "Angiostrongyliasis cantonensis (eosinophilic meningitis, Alicata's disease).", "content": "Angiostrongyliasis is an infectious disease caused by nematode parasites of the genus Angiostrongylus. The rat lung worm Angiostrongylus cantonensis, primarily a parasite of rodents, is largely responsible for human cases of eosinophilic meningitis, or meningoencerphalitis, which occurs on many Pacific islands and in Southeast Asia. The disorder, which frequently occurs in epidemic extent, is caused by invasion of the central nervous system by developing larvae of the parasite. The infection is most frequently due to ingestion of food containing the infective, third-state, larvae. Meningitic and ocular forms of the disease have been recognized. The disease has been described or referred to under a variety of synonyms. The terms eosinophilic meningitis, eosinophilic meningoencephalitis, and epidemic eosinophilic meningitis were first used to describe the disease before its etiology was known. These terms, however, lack specificity, because the eosinophilic meningitic syndrome may accompany many other parasitic as well as nonparasitic diseases of the central nervous system. Nevertheless, they are still being widely used, since in most cases only the epidemiology of the disease points to the etiologic role of A. cantonensis. Direct clinical or laboratory evidence of the etiologic agent is usually not established because reliable tests are not yet available. The term angiostrongylosis, or angiostrongyliasis, if used without the adjective, also may give rise to confusion, since the same term is applied to the pulmonary infection of dogs by A. vasorum and might be used for infection by any other Angiostrongylus species. Even the term cerebral or ocular angiostrongyliasis may prove in the future to have similar disadvantages. The scientifically correct term angiostrongyliasis cantonensis has been used recently. It is sufficiently specific and formed in analogy to the names of other parasitic diseases of man, like schistosomiasis japonica, schistosomiasis mansoni, schistosomiasis haematobia. For the murine infection, as well as for the disease produced experimentally in animals, the term angiostrongylosis cantonensis ought to be reserved because of the preferential use by veterinarians of the ending osis for designation of pathological changes produced in animals by parasites. After the recent discovery of A. costaricensis, another rat parasite causing human disease in Costa Rica, it becomes necessary to distinguish between angiostrongyliasis cantonensis (eosinophilic meningitis) and angiostrongyliasis costaricensis (intra-abdominal eosinophilic granulomatosis). A potential disadvantage of these terms may be encountered in case of a systemic revision or reclassification of the parasite. This has happened at intervals. A. cantonensis was named Pulmonema cantonensis by its discoverer and later was described under the name Haemostrongylus ratti.", "contents": "Angiostrongyliasis cantonensis (eosinophilic meningitis, Alicata's disease). Angiostrongyliasis is an infectious disease caused by nematode parasites of the genus Angiostrongylus. The rat lung worm Angiostrongylus cantonensis, primarily a parasite of rodents, is largely responsible for human cases of eosinophilic meningitis, or meningoencerphalitis, which occurs on many Pacific islands and in Southeast Asia. The disorder, which frequently occurs in epidemic extent, is caused by invasion of the central nervous system by developing larvae of the parasite. The infection is most frequently due to ingestion of food containing the infective, third-state, larvae. Meningitic and ocular forms of the disease have been recognized. The disease has been described or referred to under a variety of synonyms. The terms eosinophilic meningitis, eosinophilic meningoencephalitis, and epidemic eosinophilic meningitis were first used to describe the disease before its etiology was known. These terms, however, lack specificity, because the eosinophilic meningitic syndrome may accompany many other parasitic as well as nonparasitic diseases of the central nervous system. Nevertheless, they are still being widely used, since in most cases only the epidemiology of the disease points to the etiologic role of A. cantonensis. Direct clinical or laboratory evidence of the etiologic agent is usually not established because reliable tests are not yet available. The term angiostrongylosis, or angiostrongyliasis, if used without the adjective, also may give rise to confusion, since the same term is applied to the pulmonary infection of dogs by A. vasorum and might be used for infection by any other Angiostrongylus species. Even the term cerebral or ocular angiostrongyliasis may prove in the future to have similar disadvantages. The scientifically correct term angiostrongyliasis cantonensis has been used recently. It is sufficiently specific and formed in analogy to the names of other parasitic diseases of man, like schistosomiasis japonica, schistosomiasis mansoni, schistosomiasis haematobia. For the murine infection, as well as for the disease produced experimentally in animals, the term angiostrongylosis cantonensis ought to be reserved because of the preferential use by veterinarians of the ending osis for designation of pathological changes produced in animals by parasites. After the recent discovery of A. costaricensis, another rat parasite causing human disease in Costa Rica, it becomes necessary to distinguish between angiostrongyliasis cantonensis (eosinophilic meningitis) and angiostrongyliasis costaricensis (intra-abdominal eosinophilic granulomatosis). A potential disadvantage of these terms may be encountered in case of a systemic revision or reclassification of the parasite. This has happened at intervals. A. cantonensis was named Pulmonema cantonensis by its discoverer and later was described under the name Haemostrongylus ratti."}
{"id": "PMID:1095294", "title": "Cerebral Malaria.", "content": "Cerebral malaria is an acute diffuse encephalopathy associated only with Plasmodium falciparum. It is probably a consequence of the rapid proliferation of the parasites in the body of man in relation to red cell invasion, and results in stagnation of blood flow in cerebralcapillaries with thromobotic occlusion of large numbers of cerebral capillaries. The subsequent cerebral pathology is cerebral infarction with haemorrhage and cerebral oedema. The wide prevalence of P. falciparum in highly endemic areas results in daily challenges to patients from several infected mosquitoes. It is thus important to understand the characteristics of P. falciparum, since this is one of the most important protozoan parasites of man and severe infection from it constitutes one of the few real clinical emergencies in tropical medicine. One of the more important aspects of the practice of medicine in the tropics is to establish a good understanding of the pattern of medical practice in that area. This applies to malaria as well as to other diseases. The neophyte might be somewhat surprised to learn, for example that an experienced colleague who lives in a holoendemic malarious area such as West Africa, sees no cerebral malaria. But the explanation is simple when the doctor concerned has a practice which involves treating adults only. Cerebral malaria is rare in adults, because in highly endemic areas, by the age of 1 year most of the infants in a group under study have already experienced their first falciparum infection. By the time they reach adult life, they have a solid immunity against severe falciparum infections. In fact, \"clinical malaria\" could occur in such a group under only two circumstances: 1) in pregnancy, a patent infection with P. falciparum might develop, probably due to an IgG drain across the placenta to the foetus;2) in an individual who has constantly taken antimalarials and who may have an immunity at such a low level that when antimalarial therapy is interrupted, clinical malaria might ensue. The above examples emphasise the paramount importance of the clinician dealing with malaria having some insight into the complex immunity processes operative in the human host; these have been reviewed by McGregor.", "contents": "Cerebral Malaria. Cerebral malaria is an acute diffuse encephalopathy associated only with Plasmodium falciparum. It is probably a consequence of the rapid proliferation of the parasites in the body of man in relation to red cell invasion, and results in stagnation of blood flow in cerebralcapillaries with thromobotic occlusion of large numbers of cerebral capillaries. The subsequent cerebral pathology is cerebral infarction with haemorrhage and cerebral oedema. The wide prevalence of P. falciparum in highly endemic areas results in daily challenges to patients from several infected mosquitoes. It is thus important to understand the characteristics of P. falciparum, since this is one of the most important protozoan parasites of man and severe infection from it constitutes one of the few real clinical emergencies in tropical medicine. One of the more important aspects of the practice of medicine in the tropics is to establish a good understanding of the pattern of medical practice in that area. This applies to malaria as well as to other diseases. The neophyte might be somewhat surprised to learn, for example that an experienced colleague who lives in a holoendemic malarious area such as West Africa, sees no cerebral malaria. But the explanation is simple when the doctor concerned has a practice which involves treating adults only. Cerebral malaria is rare in adults, because in highly endemic areas, by the age of 1 year most of the infants in a group under study have already experienced their first falciparum infection. By the time they reach adult life, they have a solid immunity against severe falciparum infections. In fact, \"clinical malaria\" could occur in such a group under only two circumstances: 1) in pregnancy, a patent infection with P. falciparum might develop, probably due to an IgG drain across the placenta to the foetus;2) in an individual who has constantly taken antimalarials and who may have an immunity at such a low level that when antimalarial therapy is interrupted, clinical malaria might ensue. The above examples emphasise the paramount importance of the clinician dealing with malaria having some insight into the complex immunity processes operative in the human host; these have been reviewed by McGregor."}
{"id": "PMID:1095295", "title": "Patterns of Amyotrophic Lateral Sclerosis and Parkinsonism-Dementia on Guam.", "content": "The striking concentration of amyotrophic lateral sclerosis (ALS) and parkinsonismdementia (PD) among the Chamorro people of Guam has attracted attention since the early 1950's. Extensive studies conducted over the past 20 years have not revealed the causes of these diseases and the reasons for their remarkable concentrations on Guam. Approximately 1 in 10 Guamanian deaths over age 25 is from ALS, and another 1 in 10 is from PD. ALS still occurs approximately 50 times more frequently on Guam than in the continental United States. Advances in virologic and biochemical techniques, and long years of study, appear to be leading us to a point where some of the problems will be resolved. We shall attempt to review the pertinent background information and summarize the data and observations up to the present time.", "contents": "Patterns of Amyotrophic Lateral Sclerosis and Parkinsonism-Dementia on Guam. The striking concentration of amyotrophic lateral sclerosis (ALS) and parkinsonismdementia (PD) among the Chamorro people of Guam has attracted attention since the early 1950's. Extensive studies conducted over the past 20 years have not revealed the causes of these diseases and the reasons for their remarkable concentrations on Guam. Approximately 1 in 10 Guamanian deaths over age 25 is from ALS, and another 1 in 10 is from PD. ALS still occurs approximately 50 times more frequently on Guam than in the continental United States. Advances in virologic and biochemical techniques, and long years of study, appear to be leading us to a point where some of the problems will be resolved. We shall attempt to review the pertinent background information and summarize the data and observations up to the present time."}
{"id": "PMID:1095299", "title": "Viral-antibody complexes in canine adenovirus type 1 (CAV-1)ocular lesions: leukocyte chemotaxis and enzyme release.", "content": "Canine adenovirus-type 1 (CAV-1)-antibody complexes caused severe anterior uveitis with corneal edema (\"blue eye\") when injected into the anterior chamber of normal dogs. The response of the anterior uvea to such immune complexes (IC) was similar to the spontaneously occurring disease. In the presence of complement (C'), IC caused release of neutrophile chemotactic factors. Following phagocytosis of IC-C' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in the surrounding medium. Membrane bound viral aggregates, presumably IC, were common in neutrophiles and in macrophages that had infiltrated the anterior chamber of opaque eyes that occurred after intravenous (IV) inoculation with attenuated CAV-1. These data were incorporated into a postulated scheme for the pathogenesis of CAV-1 uveitis with corneal edema.", "contents": "Viral-antibody complexes in canine adenovirus type 1 (CAV-1)ocular lesions: leukocyte chemotaxis and enzyme release. Canine adenovirus-type 1 (CAV-1)-antibody complexes caused severe anterior uveitis with corneal edema (\"blue eye\") when injected into the anterior chamber of normal dogs. The response of the anterior uvea to such immune complexes (IC) was similar to the spontaneously occurring disease. In the presence of complement (C'), IC caused release of neutrophile chemotactic factors. Following phagocytosis of IC-C' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in the surrounding medium. Membrane bound viral aggregates, presumably IC, were common in neutrophiles and in macrophages that had infiltrated the anterior chamber of opaque eyes that occurred after intravenous (IV) inoculation with attenuated CAV-1. These data were incorporated into a postulated scheme for the pathogenesis of CAV-1 uveitis with corneal edema."}
{"id": "PMID:1095302", "title": "The planning, operation, and function of a clinical laboratory in a teaching hospital.", "content": "Shortly after World War I, laboratories began to grow in number and complexity in the hospitals throughout the United States. Need dictated the funding and therefore, expansion of these laboratories. In general, very little overall planning was introduced into the development of these laboratories but rather empiricism and reaction to pressure dictated the day-to-day, week-by-week development. Since a great deal of the teaching at that time was done on a one-by-one basis with laboratory learners, and since much of the laboratory work was done by physicians and used directly upon the patient, there was very little need for considering the laboratory as a more global resource for the teaching institution. As the science of medicine advanced and as the diversity and complexity of laboratory occupations increased, it was necessary to place more stress upon careful planning and operation of the clinical laboratory in the teaching institution. The laboratory was approached not only as a training area in its own right but as an adjunct to the undergraduate and postgraduate medical education occurring in the teaching institution. It became paramount then to consider the detail planning necessary to bring about a structural and functional entity that could respond to these more global teaching needs. It was imperative that the laboratory itself respond to its role and responsibility in the tripartite function of a teaching institution -- research, education, and service. In any good planning environment, effort must be expended towards setting goals and objectives; analytical methods applied to making assumptions and establishing premises. Alternate means for achieving goals and objectives were developed. Energy was expended in making forecasts and projecting results. Alternate means were sorted out and choices were made. Implementation guidelines were designed and an evaluation and feedback mechanism was instituted. In more recent years, we have attempted to apply these steps in planning to the development of laboratories and to the installation of operational procedures which would allow the clinical laboratory to truly function as a teaching unit and as a vital resource for the total training program of the institution in which it is housed.", "contents": "The planning, operation, and function of a clinical laboratory in a teaching hospital. Shortly after World War I, laboratories began to grow in number and complexity in the hospitals throughout the United States. Need dictated the funding and therefore, expansion of these laboratories. In general, very little overall planning was introduced into the development of these laboratories but rather empiricism and reaction to pressure dictated the day-to-day, week-by-week development. Since a great deal of the teaching at that time was done on a one-by-one basis with laboratory learners, and since much of the laboratory work was done by physicians and used directly upon the patient, there was very little need for considering the laboratory as a more global resource for the teaching institution. As the science of medicine advanced and as the diversity and complexity of laboratory occupations increased, it was necessary to place more stress upon careful planning and operation of the clinical laboratory in the teaching institution. The laboratory was approached not only as a training area in its own right but as an adjunct to the undergraduate and postgraduate medical education occurring in the teaching institution. It became paramount then to consider the detail planning necessary to bring about a structural and functional entity that could respond to these more global teaching needs. It was imperative that the laboratory itself respond to its role and responsibility in the tripartite function of a teaching institution -- research, education, and service. In any good planning environment, effort must be expended towards setting goals and objectives; analytical methods applied to making assumptions and establishing premises. Alternate means for achieving goals and objectives were developed. Energy was expended in making forecasts and projecting results. Alternate means were sorted out and choices were made. Implementation guidelines were designed and an evaluation and feedback mechanism was instituted. In more recent years, we have attempted to apply these steps in planning to the development of laboratories and to the installation of operational procedures which would allow the clinical laboratory to truly function as a teaching unit and as a vital resource for the total training program of the institution in which it is housed."}
{"id": "PMID:1095303", "title": "Physiological changes in blood volume.", "content": "Measurement of blood volume is an important clinical tool in establishing a diagnosis as in polycythemia vera, in assessing the true significance of a low blood count in a patient with splenomegaly, and in evaluating a bleeding patient. In theory, blood volume measurements should be of great value in treating acute blood loss, but this is often too time-consuming to be practicable. The main value of blood volume studies so far has been in clinical research where it is essential for correct interpretation of the peripheral blood measurements. Estimates using the hematocrit (packed cell volume) could be misleading since this measurement does not take into account total blood volume and changes in the plasma volume. A variety of isotopes that tag the red blood cell have been used to measure total blood volume and red cell mass. Most commonly used at present is autologous 51-Cr labeled red cells, thus eliminating the risk of hepatitis in transfusion to the subjects. Radio labeled albumin and other plasma proteins were used to measure plasma volume. A large molecular protein is ideal to eliminate overestimation from extravascular diffusion of labeled material for accurate determination of plasma volume. Physiologic changes in the newborn, in pregnant women, during exercise, bed rest, and altitude in the nonpathologic state are discussed. Studies in the normal subject's adaptation in different physiologic conditions have revealed a variety of factors that regulate blood volume.", "contents": "Physiological changes in blood volume. Measurement of blood volume is an important clinical tool in establishing a diagnosis as in polycythemia vera, in assessing the true significance of a low blood count in a patient with splenomegaly, and in evaluating a bleeding patient. In theory, blood volume measurements should be of great value in treating acute blood loss, but this is often too time-consuming to be practicable. The main value of blood volume studies so far has been in clinical research where it is essential for correct interpretation of the peripheral blood measurements. Estimates using the hematocrit (packed cell volume) could be misleading since this measurement does not take into account total blood volume and changes in the plasma volume. A variety of isotopes that tag the red blood cell have been used to measure total blood volume and red cell mass. Most commonly used at present is autologous 51-Cr labeled red cells, thus eliminating the risk of hepatitis in transfusion to the subjects. Radio labeled albumin and other plasma proteins were used to measure plasma volume. A large molecular protein is ideal to eliminate overestimation from extravascular diffusion of labeled material for accurate determination of plasma volume. Physiologic changes in the newborn, in pregnant women, during exercise, bed rest, and altitude in the nonpathologic state are discussed. Studies in the normal subject's adaptation in different physiologic conditions have revealed a variety of factors that regulate blood volume."}
{"id": "PMID:1095305", "title": "Prodigiosin-like pigments.", "content": "Prodigiosin, the bright red tripyrrole pigment from Serratia marcescens, has also been identified in Pseudomonas magnesiorubra, Vibrio psychroerythrus, and two Gram-negative rod-shaped mesophilic marine bacteria not members of the genus Serratia. Prodigiosin is sometimes bound to proteins; thus, extracts may require acid treatment before isolation of the pigment. Higher homologs of prodigiosin have been detected by mass spectroscopy. A mutant strain of S. marcescens produced nor-prodigiosin, in which the methoxy group of prodigiosin is replaced by a hydroxy group. Another mutant strain produced a blue tetrapyrrole pigment whose structure is a dimer of prodigiosin's rings A and B. Three novel biosynthetic analogs of prodigiosin have been obtained using a colorless mutant which does make rings A and B but not ring C and which can couple rings A and B with some added monopyrroles similar to ring C. The structures of three prodiginine (prodigiosin-like) pigments from streptomyces have been elucidated. All have the methoxytripyrrole aromatic nucleus of prodigiosin and all have an 11 carbon aliphatic side chain attached at carbon 2 of ring C. In two of the pigments the side chain is also linked to another carbon of ring C. The earlier literature about prodiginine pigments from actinomycetes has been interpreted and evaluated in light of the most recent findings. The structure elucidation of six prodiginine pigments from Actinomadurae (Nocardiae) has been completed. Only one, undecylprodiginine, is the same as from a streptomycete. For three of the six pigments, nine carbon side chains are observed and in four of them the side chain is attached to carbon 5 of ring A as well as carbon 2 of ring C so that a large ring is formed which includes the three pyrrole moieties. A section on identification summarized useful methods and presents information with which any known prodiginine pigment can be identified. The final step in the biosynthesis of prodigiosin was known to be the coupling of methoxybipyrrolecarboxaldehyde (rings A and B) with methylpentylpyrrole (ring C). Recent work using 13C-labeled precursors and Fourier transform 13C nuclear magnetic resonance has shown the pattern of incorporation for acetate, proline, glycine, serine alanine, and methionine into prodigiosin. Each pyrrole ring is constructed in a different way. Two of the streptomyces pigments have also been investigated; the pattern of incorporation is similar to that for prodigiosin. The biological activities of some prodiginine pigments are summarized. All show activity against several Gram-positive bacteria; some have anti-malarial activity. Prodigiosin has been tested clinically against coccidioidomycosis.", "contents": "Prodigiosin-like pigments. Prodigiosin, the bright red tripyrrole pigment from Serratia marcescens, has also been identified in Pseudomonas magnesiorubra, Vibrio psychroerythrus, and two Gram-negative rod-shaped mesophilic marine bacteria not members of the genus Serratia. Prodigiosin is sometimes bound to proteins; thus, extracts may require acid treatment before isolation of the pigment. Higher homologs of prodigiosin have been detected by mass spectroscopy. A mutant strain of S. marcescens produced nor-prodigiosin, in which the methoxy group of prodigiosin is replaced by a hydroxy group. Another mutant strain produced a blue tetrapyrrole pigment whose structure is a dimer of prodigiosin's rings A and B. Three novel biosynthetic analogs of prodigiosin have been obtained using a colorless mutant which does make rings A and B but not ring C and which can couple rings A and B with some added monopyrroles similar to ring C. The structures of three prodiginine (prodigiosin-like) pigments from streptomyces have been elucidated. All have the methoxytripyrrole aromatic nucleus of prodigiosin and all have an 11 carbon aliphatic side chain attached at carbon 2 of ring C. In two of the pigments the side chain is also linked to another carbon of ring C. The earlier literature about prodiginine pigments from actinomycetes has been interpreted and evaluated in light of the most recent findings. The structure elucidation of six prodiginine pigments from Actinomadurae (Nocardiae) has been completed. Only one, undecylprodiginine, is the same as from a streptomycete. For three of the six pigments, nine carbon side chains are observed and in four of them the side chain is attached to carbon 5 of ring A as well as carbon 2 of ring C so that a large ring is formed which includes the three pyrrole moieties. A section on identification summarized useful methods and presents information with which any known prodiginine pigment can be identified. The final step in the biosynthesis of prodigiosin was known to be the coupling of methoxybipyrrolecarboxaldehyde (rings A and B) with methylpentylpyrrole (ring C). Recent work using 13C-labeled precursors and Fourier transform 13C nuclear magnetic resonance has shown the pattern of incorporation for acetate, proline, glycine, serine alanine, and methionine into prodigiosin. Each pyrrole ring is constructed in a different way. Two of the streptomyces pigments have also been investigated; the pattern of incorporation is similar to that for prodigiosin. The biological activities of some prodiginine pigments are summarized. All show activity against several Gram-positive bacteria; some have anti-malarial activity. Prodigiosin has been tested clinically against coccidioidomycosis."}
{"id": "PMID:1095306", "title": "The therapeutic activity of diftalone in rheumatoid arthritis: crossover comparison with indomethacin.", "content": "A multi-centre double-blind crossover study was carried out in 70 hospitalised patients with rheumatoid arthritis to compare the activity of diftalone, a new anti-inflammatory agen, with that of indomethacin. Patients received either 750 mg. diftalone or 100 mg. indomethacin daily for 2 weeks and were then crossed over to the alternative treatment for a further 2 weeks. Routine clinical and laboratory assessments of disease activity were carried out before the start of the trial and after each treatment period. The results indicate that both drugs produced a favourable response and that there was no statistically significant difference between them in the parameters measure, except for the erythrocyte sedimentation rate which fell only after the administration of diftalone. Patient and physician assessments on the course of the disease were in general agreement and were favourable to the same extent for both treatments. Both products were well tolerated and there were few reports of side-effects.", "contents": "The therapeutic activity of diftalone in rheumatoid arthritis: crossover comparison with indomethacin. A multi-centre double-blind crossover study was carried out in 70 hospitalised patients with rheumatoid arthritis to compare the activity of diftalone, a new anti-inflammatory agen, with that of indomethacin. Patients received either 750 mg. diftalone or 100 mg. indomethacin daily for 2 weeks and were then crossed over to the alternative treatment for a further 2 weeks. Routine clinical and laboratory assessments of disease activity were carried out before the start of the trial and after each treatment period. The results indicate that both drugs produced a favourable response and that there was no statistically significant difference between them in the parameters measure, except for the erythrocyte sedimentation rate which fell only after the administration of diftalone. Patient and physician assessments on the course of the disease were in general agreement and were favourable to the same extent for both treatments. Both products were well tolerated and there were few reports of side-effects."}
{"id": "PMID:1095307", "title": "A controlled trial of bromhexine ('Bisolvon') in out-patients with chronic bronchitis.", "content": "Bromhexine, 16 mg. 3-times daily, was compared with placebo in a double-blind crossover trial in 41 out-patients with chronic bronchitis and irreversible airways obstruction, and who were considered to be in a steady state. Each treatment was given for 3 weeks with a week of placebo in between; in addition, all patients took oxytetracycline, 500 mg. twice daily, starting 1 week before the trial and continuing throughout the duration of it. All patients completed the trial but the results from 5 were excluded because during the first 3 weeks they developed an influenza-like illness accompanied by a decrease in FEV1,and peak expiratory flow rate. Results from the remaining 36 patients showed a statistically significant reduction in phlegm stickiness (p smaller than 0.05) as judged by the patient, and a significant improvement in overall clinical state (p smaller than 0.05) as judged by the physician during bromhexine treatment, but no significant change in symptoms like cough, chest tightness, ease of breathing or sputum volume, peak expiratory flow rate and FEV1 etc. Five patients reported 6 side-effects, 3 with bromhexine, 1 with placebo and 1 with both treatments.", "contents": "A controlled trial of bromhexine ('Bisolvon') in out-patients with chronic bronchitis. Bromhexine, 16 mg. 3-times daily, was compared with placebo in a double-blind crossover trial in 41 out-patients with chronic bronchitis and irreversible airways obstruction, and who were considered to be in a steady state. Each treatment was given for 3 weeks with a week of placebo in between; in addition, all patients took oxytetracycline, 500 mg. twice daily, starting 1 week before the trial and continuing throughout the duration of it. All patients completed the trial but the results from 5 were excluded because during the first 3 weeks they developed an influenza-like illness accompanied by a decrease in FEV1,and peak expiratory flow rate. Results from the remaining 36 patients showed a statistically significant reduction in phlegm stickiness (p smaller than 0.05) as judged by the patient, and a significant improvement in overall clinical state (p smaller than 0.05) as judged by the physician during bromhexine treatment, but no significant change in symptoms like cough, chest tightness, ease of breathing or sputum volume, peak expiratory flow rate and FEV1 etc. Five patients reported 6 side-effects, 3 with bromhexine, 1 with placebo and 1 with both treatments."}
{"id": "PMID:1095308", "title": "Pizotifen (BC 105) in migraine prophylaxis.", "content": "Pizotifen, an antaminic, was investigated in a double-blind placebo controlled trial to evaluate its efficacy in migraine prophylaxis. It was found to be and effective prophylactic in a proportion of migraine patients, reducing the frequency as well as the severity of headaches. Some patients showed weight gain. Several patients have continued treatment for up to 2 years with good control of their migraine and a reduction in the amount of medicament required for the acute attack.", "contents": "Pizotifen (BC 105) in migraine prophylaxis. Pizotifen, an antaminic, was investigated in a double-blind placebo controlled trial to evaluate its efficacy in migraine prophylaxis. It was found to be and effective prophylactic in a proportion of migraine patients, reducing the frequency as well as the severity of headaches. Some patients showed weight gain. Several patients have continued treatment for up to 2 years with good control of their migraine and a reduction in the amount of medicament required for the acute attack."}
{"id": "PMID:1095309", "title": "Clinical trial of a low dose combined oral contraceptive ('Ovranette').", "content": "A combination of 150 mug. d-norgestrel with 30 mug. ethinyl oestradiol was administered to 120 women for a total of 767 cycles. No pregnancies occurred and cycle control was good, 99% of the cycles being within the range of 28 plus or minus 3 days. Breakthrough bleeding occurred in 7.2% of the cycles, spotting in 8.9% and amenorphoea in 0.8%. Side-effects were minimal.", "contents": "Clinical trial of a low dose combined oral contraceptive ('Ovranette'). A combination of 150 mug. d-norgestrel with 30 mug. ethinyl oestradiol was administered to 120 women for a total of 767 cycles. No pregnancies occurred and cycle control was good, 99% of the cycles being within the range of 28 plus or minus 3 days. Breakthrough bleeding occurred in 7.2% of the cycles, spotting in 8.9% and amenorphoea in 0.8%. Side-effects were minimal."}
{"id": "PMID:1095310", "title": "Six-day clotrimazole therapy in vaginal candidosis.", "content": "Two hundred and two patients with symptoms, signs and mycological evidence of Candida vaginitis were entered in a single-blind trial comparing 6-day clotrimazole therapy with a control group receiving 6 days' nystatin therapy. Adequate data was collected on 69 clotrimazole and 72 nystatin treated patients for analysis. Judged by both the severity of symptoms and signs and the number of patients with negative mycological findings, 6-day clotrimazole therapy produced statistically significantly better results than obtained in the control group. When reviewed 4 weeks after starting therapy, 93% of the clotrimazole treated patinets had culturally negative results for Candida compared with 74% of the patients treated with nystatin. It is concluded that 6-day clotrimazole therapy is effective in the treatment of Candida vaginitis.", "contents": "Six-day clotrimazole therapy in vaginal candidosis. Two hundred and two patients with symptoms, signs and mycological evidence of Candida vaginitis were entered in a single-blind trial comparing 6-day clotrimazole therapy with a control group receiving 6 days' nystatin therapy. Adequate data was collected on 69 clotrimazole and 72 nystatin treated patients for analysis. Judged by both the severity of symptoms and signs and the number of patients with negative mycological findings, 6-day clotrimazole therapy produced statistically significantly better results than obtained in the control group. When reviewed 4 weeks after starting therapy, 93% of the clotrimazole treated patinets had culturally negative results for Candida compared with 74% of the patients treated with nystatin. It is concluded that 6-day clotrimazole therapy is effective in the treatment of Candida vaginitis."}
{"id": "PMID:1095311", "title": "Evaluation of pindolol in the treatment of hypertension.", "content": "Twenty hypertensive patients were treated for 15 weeks with pindolol, a new anti-hypertensive agent. Blood pressure while resting and after standing was reduced in all patients whether previously treated with an antihypertensive agent or not. All patients completed the study and no side-effects were reported. Laboratory tests showed no abnormalities and pindolol was very well tolerated in all patients. The dose of other antihypertensive agents was reduced in 7 patients.", "contents": "Evaluation of pindolol in the treatment of hypertension. Twenty hypertensive patients were treated for 15 weeks with pindolol, a new anti-hypertensive agent. Blood pressure while resting and after standing was reduced in all patients whether previously treated with an antihypertensive agent or not. All patients completed the study and no side-effects were reported. Laboratory tests showed no abnormalities and pindolol was very well tolerated in all patients. The dose of other antihypertensive agents was reduced in 7 patients."}
{"id": "PMID:1095319", "title": "Lampbrush chromosomes in spermatocytes of Chironomus.", "content": "In the primary spermatocytes of Chironomus pallidivittatus lampbrush chromosomes in pachytene and diplotene stages can be isolated by using a spreading technique.--In the earliest analyzable stages of the pachytene the lampbrush loops, although different in size, are identically structured, consisting of spiralized fibers covered with a thin matrix. These loops contain an average DNA length of 33 mum. They are connected by axial fibers of 6 nm in diameter at intervals of 0.15--0.07 mum without any interposing chromomeres. The axis is double-stranded.--On the basis of the DNA distribution patterns it is supposed that the loops contain those chromatid regions which are included in the bands of the polytene chromosomes of the same species. The lack of a typical RNP transcript in the loops and the finding that 98% of the total DNA is found in the loops lead to the conception that the lampbrush organization in these spermatocytes is primarily involved in the orderly course of crossing-over and in the preparation for the metaphase contraction of the chromatids.", "contents": "Lampbrush chromosomes in spermatocytes of Chironomus. In the primary spermatocytes of Chironomus pallidivittatus lampbrush chromosomes in pachytene and diplotene stages can be isolated by using a spreading technique.--In the earliest analyzable stages of the pachytene the lampbrush loops, although different in size, are identically structured, consisting of spiralized fibers covered with a thin matrix. These loops contain an average DNA length of 33 mum. They are connected by axial fibers of 6 nm in diameter at intervals of 0.15--0.07 mum without any interposing chromomeres. The axis is double-stranded.--On the basis of the DNA distribution patterns it is supposed that the loops contain those chromatid regions which are included in the bands of the polytene chromosomes of the same species. The lack of a typical RNP transcript in the loops and the finding that 98% of the total DNA is found in the loops lead to the conception that the lampbrush organization in these spermatocytes is primarily involved in the orderly course of crossing-over and in the preparation for the metaphase contraction of the chromatids."}
{"id": "PMID:1095320", "title": "The proteins of polytene chromosomes of Drosophila hydei.", "content": "It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important components of constitutive heterochromatin, which is severely underrepresented in polytene chromosomes. Chromosome morphology, including the pattern of induced puffs, is maintained throughout the mass isolation of glands and sucrose gradient purification of nuclei, as indicated by studies on temperature-shocked and control larvae. No significant alteration in the chromosomal proteins following puff induction by heat shock could be detected on analysis of the isolated protein fractions by disc gel electrophoresis. More sensitive techniques must be developed to study the apparent rearrangement or accumulation of protein at puff sites, and to elucidate the role of this protein in gene activation.", "contents": "The proteins of polytene chromosomes of Drosophila hydei. It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important components of constitutive heterochromatin, which is severely underrepresented in polytene chromosomes. Chromosome morphology, including the pattern of induced puffs, is maintained throughout the mass isolation of glands and sucrose gradient purification of nuclei, as indicated by studies on temperature-shocked and control larvae. No significant alteration in the chromosomal proteins following puff induction by heat shock could be detected on analysis of the isolated protein fractions by disc gel electrophoresis. More sensitive techniques must be developed to study the apparent rearrangement or accumulation of protein at puff sites, and to elucidate the role of this protein in gene activation."}
{"id": "PMID:1095323", "title": "Accuracy of actuarial and clinical predictions for length of stay and unauthorized absence.", "content": "Multivariate-equation predictions of length of hospital stay and probability of unauthorized absence were compared with similar predictions made by clincians and actual outcome for 167 patients drawn from a random sample of admissions during a two-month period at five state hospitals. The computer proved to be about as accurate as clinicians in predicting length of stay. Neither computer nor clinician was able to usefully predict unauthorized absence because of its infrquent occurrance. Use and improvement of such actuarial predictions within the Missouri automated data base were discussed.", "contents": "Accuracy of actuarial and clinical predictions for length of stay and unauthorized absence. Multivariate-equation predictions of length of hospital stay and probability of unauthorized absence were compared with similar predictions made by clincians and actual outcome for 167 patients drawn from a random sample of admissions during a two-month period at five state hospitals. The computer proved to be about as accurate as clinicians in predicting length of stay. Neither computer nor clinician was able to usefully predict unauthorized absence because of its infrquent occurrance. Use and improvement of such actuarial predictions within the Missouri automated data base were discussed."}
{"id": "PMID:1095330", "title": "[Postanastomotic renal-artery stenosis after renal transplantation].", "content": "A particular kind of renal-artery stenosis was observed in three of 60 renal transplantations performed between 1969 and 1974. It was always 1.0-1.5 cm distal to the anastomosis and, as histological examination demonstrated, was definitely due to vessel injury after removal of the transplant. To avoid it the proximal arterial stump should be cut off before transplantation. The postanastomotic region must be dissected free during the operation and an extended graft venoplasty or sleeve resection performed.", "contents": "[Postanastomotic renal-artery stenosis after renal transplantation]. A particular kind of renal-artery stenosis was observed in three of 60 renal transplantations performed between 1969 and 1974. It was always 1.0-1.5 cm distal to the anastomosis and, as histological examination demonstrated, was definitely due to vessel injury after removal of the transplant. To avoid it the proximal arterial stump should be cut off before transplantation. The postanastomotic region must be dissected free during the operation and an extended graft venoplasty or sleeve resection performed."}
{"id": "PMID:1095331", "title": "[Ectopic mammary gland on the back].", "content": "A supernumerary, fully developed mammary gland between both shoulder blades above the vertebral column was discovered in a 32-year-old woman when it caused symptoms. It was removed by operation. Histological examination revealed fibrous mastopathy. The only abnormal radiological feature was a spina bifida of T6 at the site of the ectopic breast.", "contents": "[Ectopic mammary gland on the back]. A supernumerary, fully developed mammary gland between both shoulder blades above the vertebral column was discovered in a 32-year-old woman when it caused symptoms. It was removed by operation. Histological examination revealed fibrous mastopathy. The only abnormal radiological feature was a spina bifida of T6 at the site of the ectopic breast."}
{"id": "PMID:1095355", "title": "Cyclic variations in the increased responsiveness of the pituitary to luteinizing hormone-releasing hormone (LHRH) induced by LHRH.", "content": "Following a single iv injection of 50 ng of LH-releasing hormone (LHRH), a small increase in plasma LH titers was observed and this response was greater on proestrus and estrus than in other stages of the cycle. When a second injection of a similar dose of the neurohormone was given one hour later, the response to the second injection was markedly enhanced on proestrus but only slightly increased at other stages of the cycle. The response to LHRH in androgenized females was similar to that observed on proestrus, and, as on proestrus, an enhanced response to a second injection occurred. Only small increases in FSH followed these injections and there was no increase in responsiveness to the second injection in any of the groups. It is postulated that rising estrogen titers can bring about a priming action of LHRH which enhances the response of the gland to a second injection on proestrus. The priming action of LHRH may magnify the response of the pituitary to endogenous decapeptide release to further increase the release of LH on the afternoon of proestrus.", "contents": "Cyclic variations in the increased responsiveness of the pituitary to luteinizing hormone-releasing hormone (LHRH) induced by LHRH. Following a single iv injection of 50 ng of LH-releasing hormone (LHRH), a small increase in plasma LH titers was observed and this response was greater on proestrus and estrus than in other stages of the cycle. When a second injection of a similar dose of the neurohormone was given one hour later, the response to the second injection was markedly enhanced on proestrus but only slightly increased at other stages of the cycle. The response to LHRH in androgenized females was similar to that observed on proestrus, and, as on proestrus, an enhanced response to a second injection occurred. Only small increases in FSH followed these injections and there was no increase in responsiveness to the second injection in any of the groups. It is postulated that rising estrogen titers can bring about a priming action of LHRH which enhances the response of the gland to a second injection on proestrus. The priming action of LHRH may magnify the response of the pituitary to endogenous decapeptide release to further increase the release of LH on the afternoon of proestrus."}
{"id": "PMID:1095356", "title": "Ovarian steroids directly alter luteinizing hormone (LH) release by pituitary homografts.", "content": "The direct role of estradiol and progesterone in sensitizing the adenohypophysis to the releasing action of LHRH (luteinizing hormone-releasing hormone) has been examined in rats bearing pituitary homografts. Groups (10-14/group) of virgin female rats (CDF) strain) were hypophysectomized, ovariectomized, and pituitaries obtained from long-term ovariectomized donors were implanted under the kidney capsule. The animals were treated on the sixth day after transplantation with sesame oil vehicle, estradiol (1.6 mug/100 g BW), progesterone (0.6 mg/100 g BW), or estradiol plus progesterone. This injection regimen was repeated 12 h later. Ninety minutes after the steroid injection on day 7 half of the animals in each group received LHRH (ip) and half received saline. Basal levels of LH in the steroid-treated groups not injected with LHRH were, in general, elevated as compared to the oil-injected group. Following LHRH, blood samples were collected by cardiac puncture under light ether anesthesia at 5, 15, 30, 60, and 90 min postinjection. Animals receiving LHRH responded with significant elevations of LH both in the presence and absence of steroids while saline was without effect. However, the magnitude of the LH peak was found to be significantly blunted by estrogen treatment. Moreover, the peak LH response to LHRH occurred 30 min following injection in all groups except the progesterone primed animals. In this group the peak LH was delayed by 30 min. These results suggest that estradiol directly inhibits the release of LH at the level of the pituitary and that the ovarian steroids, estradiol and progesterone probably interact to determine the time of optimal pituitary sensitivity to LHRH.", "contents": "Ovarian steroids directly alter luteinizing hormone (LH) release by pituitary homografts. The direct role of estradiol and progesterone in sensitizing the adenohypophysis to the releasing action of LHRH (luteinizing hormone-releasing hormone) has been examined in rats bearing pituitary homografts. Groups (10-14/group) of virgin female rats (CDF) strain) were hypophysectomized, ovariectomized, and pituitaries obtained from long-term ovariectomized donors were implanted under the kidney capsule. The animals were treated on the sixth day after transplantation with sesame oil vehicle, estradiol (1.6 mug/100 g BW), progesterone (0.6 mg/100 g BW), or estradiol plus progesterone. This injection regimen was repeated 12 h later. Ninety minutes after the steroid injection on day 7 half of the animals in each group received LHRH (ip) and half received saline. Basal levels of LH in the steroid-treated groups not injected with LHRH were, in general, elevated as compared to the oil-injected group. Following LHRH, blood samples were collected by cardiac puncture under light ether anesthesia at 5, 15, 30, 60, and 90 min postinjection. Animals receiving LHRH responded with significant elevations of LH both in the presence and absence of steroids while saline was without effect. However, the magnitude of the LH peak was found to be significantly blunted by estrogen treatment. Moreover, the peak LH response to LHRH occurred 30 min following injection in all groups except the progesterone primed animals. In this group the peak LH was delayed by 30 min. These results suggest that estradiol directly inhibits the release of LH at the level of the pituitary and that the ovarian steroids, estradiol and progesterone probably interact to determine the time of optimal pituitary sensitivity to LHRH."}
{"id": "PMID:1095362", "title": "Localization of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay.", "content": "To determine the localization of luteinizing hormone-releasing hormone (LHRH), five brains from adult male rats were serially sectioned in a cryostat at - 10 C in either the frontal, horizontal or sagittal planes. Acetic acid-ethanol extracts of each section were assayed for LHRH using radioimmunoassay (RIA) and in some cases using bioassay as well. Approximately 0.2 ng LHRH was concentrated in medial basal preoptic (MB-PO) tissue overlying the rostral portion of the optic chiasm. This LHRH appears to be associated with the organum vasculosum of the lamina terminalis and/or adjacent neural tissue. Uniform, low levels of LHRH were detected in hypothalamic tissue between the preoptic area (POA) and arcuate-median eminence (ARC-ME) region. In the ARC-ME region 2.7 ng of LHRH were concentrated primarily in the median eminence. The lateral distribution of LHRH in the ARC-ME region extended beyond the median eminence into tissue corresponding to the lateral aspect of the ventromedial nucleus. Concomitant bioassay and RIA determinations of LHRH were highly correlated. Of the sections bioassayed, only those sections containing LHRH released FSH. These results confirm the presence of LHRH in the POA and in the rostral hypothalamus of the rat brain. The possible significance of LHRH in the POA for the regulation of LH release is discussed.", "contents": "Localization of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay. To determine the localization of luteinizing hormone-releasing hormone (LHRH), five brains from adult male rats were serially sectioned in a cryostat at - 10 C in either the frontal, horizontal or sagittal planes. Acetic acid-ethanol extracts of each section were assayed for LHRH using radioimmunoassay (RIA) and in some cases using bioassay as well. Approximately 0.2 ng LHRH was concentrated in medial basal preoptic (MB-PO) tissue overlying the rostral portion of the optic chiasm. This LHRH appears to be associated with the organum vasculosum of the lamina terminalis and/or adjacent neural tissue. Uniform, low levels of LHRH were detected in hypothalamic tissue between the preoptic area (POA) and arcuate-median eminence (ARC-ME) region. In the ARC-ME region 2.7 ng of LHRH were concentrated primarily in the median eminence. The lateral distribution of LHRH in the ARC-ME region extended beyond the median eminence into tissue corresponding to the lateral aspect of the ventromedial nucleus. Concomitant bioassay and RIA determinations of LHRH were highly correlated. Of the sections bioassayed, only those sections containing LHRH released FSH. These results confirm the presence of LHRH in the POA and in the rostral hypothalamus of the rat brain. The possible significance of LHRH in the POA for the regulation of LH release is discussed."}
{"id": "PMID:1095363", "title": "Effect of alloxan on permeability and hexose transport in rat pancreatic islets.", "content": "The in vitro effect of alloxan exposure on the permeability of collagenase isolated rat pancreatic islets to sucrose, D-mannitol, and L-glucose has been investigated. Determination of changes in cell volume with a non-wash double label isotope procedure indicates that alloxan treatment exerts no measurable effect on permeability to sucrose, D-mannitol, or L-glucose as compared to nonalloxan-treated islets. In addition, neither prior exposure nor the concomitant presence of alloxan alters the rate of D-glucose or 3-0-methyl-D-glucose transport into rat pancreatic islets. It is concluded that the in vitro effect of alloxan on abolishing glucose-induced insulin release in isolated rat pancreatic islets does not appear to be the result of permeability changes to small organic molecules or alteration in the transport of D-glucose into the beta-cell.", "contents": "Effect of alloxan on permeability and hexose transport in rat pancreatic islets. The in vitro effect of alloxan exposure on the permeability of collagenase isolated rat pancreatic islets to sucrose, D-mannitol, and L-glucose has been investigated. Determination of changes in cell volume with a non-wash double label isotope procedure indicates that alloxan treatment exerts no measurable effect on permeability to sucrose, D-mannitol, or L-glucose as compared to nonalloxan-treated islets. In addition, neither prior exposure nor the concomitant presence of alloxan alters the rate of D-glucose or 3-0-methyl-D-glucose transport into rat pancreatic islets. It is concluded that the in vitro effect of alloxan on abolishing glucose-induced insulin release in isolated rat pancreatic islets does not appear to be the result of permeability changes to small organic molecules or alteration in the transport of D-glucose into the beta-cell."}
{"id": "PMID:1095364", "title": "Biological aspects of cytochrome P450 and associated hydroxylation reactions.", "content": "A review is made of the distribution and functions of cytochrome P450 in various tissues. \"Normal\" levels of the enzyme are tabulated and factors inducing higher levels are discussed. Evidence suggestive of the existence of different species of the enzyme is discussed, as is the hyothesis that genetically defective (or absent) isoenzymes of cytochrome P450 can provide an explanation for the clinical findings caused by the adrenogenital syndrome.", "contents": "Biological aspects of cytochrome P450 and associated hydroxylation reactions. A review is made of the distribution and functions of cytochrome P450 in various tissues. \"Normal\" levels of the enzyme are tabulated and factors inducing higher levels are discussed. Evidence suggestive of the existence of different species of the enzyme is discussed, as is the hyothesis that genetically defective (or absent) isoenzymes of cytochrome P450 can provide an explanation for the clinical findings caused by the adrenogenital syndrome."}
{"id": "PMID:1095365", "title": "Some reflections on clinical research.", "content": "The place of clinical research in the veterinary profession is discussed against the author's personal experiences and in the context of how research workers, clinicians, teachers and veterinary students might be brought into a more cohesive unit through the development of a Faculty of clinical research and experimental medicine. It is argued that students should receive training in research and teaching and that  efforts should be made to break down the attitude of \"them\" and \"us\" which tends to separate the clinicians and academics.", "contents": "Some reflections on clinical research. The place of clinical research in the veterinary profession is discussed against the author's personal experiences and in the context of how research workers, clinicians, teachers and veterinary students might be brought into a more cohesive unit through the development of a Faculty of clinical research and experimental medicine. It is argued that students should receive training in research and teaching and that  efforts should be made to break down the attitude of \"them\" and \"us\" which tends to separate the clinicians and academics."}
{"id": "PMID:1095366", "title": "Surgical repair of cleft palate in the horse.", "content": "Surgical repair of a cleft palate was carried out in three horses. Mandibular symphisotomy allowed adequate exposure of the defect. The first subject, a young foal died from inhalation pneumonia but the other two made satisfactory recoveries. The problems of closure of the lip and symphysis are discussed.", "contents": "Surgical repair of cleft palate in the horse. Surgical repair of a cleft palate was carried out in three horses. Mandibular symphisotomy allowed adequate exposure of the defect. The first subject, a young foal died from inhalation pneumonia but the other two made satisfactory recoveries. The problems of closure of the lip and symphysis are discussed."}
{"id": "PMID:1095367", "title": "Maintenance of fertility in the horse including artificial insemination.", "content": "A high fertility  rate depends on many different factors and is always related to inheritance and enviorment. The successful feritly control system in the German Thoroughbred breeding industry shows that fertility can be increased by good management and  veterinary supervision. The  insemination of horses with frozen semen is discussed. Replacement of natural service by A.I. with frozen semen is not generally  accepted in horsebreeding, as the conditions are entirely different from cattle breeding. However, there are several ways in which A.I. can be assistance in stud management.", "contents": "Maintenance of fertility in the horse including artificial insemination. A high fertility  rate depends on many different factors and is always related to inheritance and enviorment. The successful feritly control system in the German Thoroughbred breeding industry shows that fertility can be increased by good management and  veterinary supervision. The  insemination of horses with frozen semen is discussed. Replacement of natural service by A.I. with frozen semen is not generally  accepted in horsebreeding, as the conditions are entirely different from cattle breeding. However, there are several ways in which A.I. can be assistance in stud management."}
{"id": "PMID:1095368", "title": "Structure and function of the genome of coliphage T5. Transcription in vitro of the \"nicked\" and \"nick-free\" T5+ DNA.", "content": "Purified T5+ DNA in nicked form and after repair of the specific single-strand interruptions with DNA ligase was used in transcription studies using Escherichia coli RNA polymerase (holoenzyme) in the presence and absence of E. coli termination protein rho. The transcriptional products were analyzed with respect to their size distribution and the sequences transcribed from the different templates. The results indicate that the single-strand breaks in the DNA of bacteriophage T5, though in genetically defined positions, do not have any specific effect on transcription in vitro. Furthermore, the E. coli rho protein, although it depresses net RNA synthesis and reduces the average molecular weight of the transcripts, seems to act in a non-specific way in this system.", "contents": "Structure and function of the genome of coliphage T5. Transcription in vitro of the \"nicked\" and \"nick-free\" T5+ DNA. Purified T5+ DNA in nicked form and after repair of the specific single-strand interruptions with DNA ligase was used in transcription studies using Escherichia coli RNA polymerase (holoenzyme) in the presence and absence of E. coli termination protein rho. The transcriptional products were analyzed with respect to their size distribution and the sequences transcribed from the different templates. The results indicate that the single-strand breaks in the DNA of bacteriophage T5, though in genetically defined positions, do not have any specific effect on transcription in vitro. Furthermore, the E. coli rho protein, although it depresses net RNA synthesis and reduces the average molecular weight of the transcripts, seems to act in a non-specific way in this system."}
{"id": "PMID:1095369", "title": "Glucose catabolite repression in Escherichia coli K12 mutants defective in methyl-alpha-d-glucoside transport.", "content": "1. Two spontaneous Escherichia coli K12 mutants resistant to glucose catabolite repression were isolated using minimal agar plates with methyl alpha-D-glucoside. Mutants grow well on glucose and mannitol. 2. Glucose does not inhibit the inducible enzyme synthesis in isolated mutants: mutant cell (in contrast to parent cells) produce high levels of beta-galactosidase and L-tryptophanase under the conditions of glucose catabolite repression. 3. The isolated mutants are negative in methyl-alpha-D-glucoside transport; glucose uptake is not severely damaged. But the mutants (named tgl, transport of glucose) retained the ability to phosphorylate methyl alpha-D-glucoside in vitro at the expense of phosphoenolpyruvate. 4. The tgl mutation is cotransduced with purB and pyrC markers, i.e. locates near 24 min of the E. coli chromosome map. 5. It is thought that E. coli cells possess two glucose transport systems. The first one is represented by the glucose-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system. The second glucose transport system (coded for tgl gene) functions as permease and possesses high affinity to methyl alpha-D-glucoside. The integrity of glucose permease determine the sensitivity of the cell to glucose catabolite repression.", "contents": "Glucose catabolite repression in Escherichia coli K12 mutants defective in methyl-alpha-d-glucoside transport. 1. Two spontaneous Escherichia coli K12 mutants resistant to glucose catabolite repression were isolated using minimal agar plates with methyl alpha-D-glucoside. Mutants grow well on glucose and mannitol. 2. Glucose does not inhibit the inducible enzyme synthesis in isolated mutants: mutant cell (in contrast to parent cells) produce high levels of beta-galactosidase and L-tryptophanase under the conditions of glucose catabolite repression. 3. The isolated mutants are negative in methyl-alpha-D-glucoside transport; glucose uptake is not severely damaged. But the mutants (named tgl, transport of glucose) retained the ability to phosphorylate methyl alpha-D-glucoside in vitro at the expense of phosphoenolpyruvate. 4. The tgl mutation is cotransduced with purB and pyrC markers, i.e. locates near 24 min of the E. coli chromosome map. 5. It is thought that E. coli cells possess two glucose transport systems. The first one is represented by the glucose-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system. The second glucose transport system (coded for tgl gene) functions as permease and possesses high affinity to methyl alpha-D-glucoside. The integrity of glucose permease determine the sensitivity of the cell to glucose catabolite repression."}
{"id": "PMID:1095370", "title": "Nuclease digestion of synthetase x tRNA complexes.", "content": "Phenylalanyl-tRNA and seryl-tRNA synthetase protect strongly though not completely their cognate tRNAs against nuclease attack, as had been shown previously. In an investigation of the mechanism of protection it was demonstrated that the low susceptibility of phenylalanyl-tRNA-synthetase x tRNA-Phe complexes to nucleases is due to free tRNA present in equilibrium with synthetase. The equilibrium can be shifted by an excess of synthetase or by dilution of the complex. It therefore appears that synthetase competes with the nuclease for free tRNA. Degradation of the complex is low, however, because under the conditions of partial digestion the synthetase has a greater affinity for the tRNA than does the nuclease. Fragmented tRNAs, as they are formed during partial nuclease digestion, bind to synthetase to different degrees. tRNA-Phe with a lesion in the dihydrouridine loop binds very poorly whereas a nick in the anticodon loop reduces the strength of binding to a much lesser extent. In a systematic study of the stoichiometry of protection it was confirmed that under standard conditions one phenylalanyl-tRNA synthetase protects one tRNA-Phe and one seryl-tRNA synthetase two tRNA-Ser molecules against nuclease attack. Under certain conditions, however, (concentration of the complex higher than 10 mu-M, or alternately in buffers of low ionic strength) it is observed that phenylalanyl-tRNA synthetase binds up to 1.6 molecules tRNA-Phe. In the serine system, these special conditions do not affect the binding properties of seryl-tRNA synthetase.", "contents": "Nuclease digestion of synthetase x tRNA complexes. Phenylalanyl-tRNA and seryl-tRNA synthetase protect strongly though not completely their cognate tRNAs against nuclease attack, as had been shown previously. In an investigation of the mechanism of protection it was demonstrated that the low susceptibility of phenylalanyl-tRNA-synthetase x tRNA-Phe complexes to nucleases is due to free tRNA present in equilibrium with synthetase. The equilibrium can be shifted by an excess of synthetase or by dilution of the complex. It therefore appears that synthetase competes with the nuclease for free tRNA. Degradation of the complex is low, however, because under the conditions of partial digestion the synthetase has a greater affinity for the tRNA than does the nuclease. Fragmented tRNAs, as they are formed during partial nuclease digestion, bind to synthetase to different degrees. tRNA-Phe with a lesion in the dihydrouridine loop binds very poorly whereas a nick in the anticodon loop reduces the strength of binding to a much lesser extent. In a systematic study of the stoichiometry of protection it was confirmed that under standard conditions one phenylalanyl-tRNA synthetase protects one tRNA-Phe and one seryl-tRNA synthetase two tRNA-Ser molecules against nuclease attack. Under certain conditions, however, (concentration of the complex higher than 10 mu-M, or alternately in buffers of low ionic strength) it is observed that phenylalanyl-tRNA synthetase binds up to 1.6 molecules tRNA-Phe. In the serine system, these special conditions do not affect the binding properties of seryl-tRNA synthetase."}
{"id": "PMID:1095371", "title": "Studies on bacteriophage M13 DNA. 2. The gene order of the M13 genome.", "content": "The double-stranded replicative form DNA of bacteriophage M13 was cleaved into 13 specific fragments by the restriction endonuclease from Haemophilus aphirophilus. The individual DNA fragments from wild-type replicative form molecules were then annealed to circular, single-stranded DNAs of phage M13, bearing amber mutations as genetic markers. When such DNA hybrids infected competent Escherichia coli cells, only those duplexes which were genetically heterozygous gave rise to wild-type phages in the progeny. In this way, the genetic markers carried on the individual DNA fragments could be determined. In addition, marker rescue in each gene was obtained with the 10 specific fragments of M13 replicative form DNA, produced by cleavage with the restriction endonuclease from Haemophilus aegyptius. From these results and the enzyme cleavage maps of both types of restriction fragments a distribution of genetic markers along the physical map could be obtained, which allowed an arrangement of M13 genes into a genetic map. Evidence is presented that the gene order of M13 is: IV-(I,VI)-III-VIII-VII-V-II with II and IV being contiguous on the circular map.", "contents": "Studies on bacteriophage M13 DNA. 2. The gene order of the M13 genome. The double-stranded replicative form DNA of bacteriophage M13 was cleaved into 13 specific fragments by the restriction endonuclease from Haemophilus aphirophilus. The individual DNA fragments from wild-type replicative form molecules were then annealed to circular, single-stranded DNAs of phage M13, bearing amber mutations as genetic markers. When such DNA hybrids infected competent Escherichia coli cells, only those duplexes which were genetically heterozygous gave rise to wild-type phages in the progeny. In this way, the genetic markers carried on the individual DNA fragments could be determined. In addition, marker rescue in each gene was obtained with the 10 specific fragments of M13 replicative form DNA, produced by cleavage with the restriction endonuclease from Haemophilus aegyptius. From these results and the enzyme cleavage maps of both types of restriction fragments a distribution of genetic markers along the physical map could be obtained, which allowed an arrangement of M13 genes into a genetic map. Evidence is presented that the gene order of M13 is: IV-(I,VI)-III-VIII-VII-V-II with II and IV being contiguous on the circular map."}
{"id": "PMID:1095372", "title": "The effect of tRNA derivatives bound with natural or synthetic mRNA on the interaction of Escherichia coli ribosomes with colicin E3.", "content": "Ribosomal binding complexes directed by poly(U) or T4 mRNA were formed with aminoacyl-tRNA or its derivatives bound to predominantly the P or A binding site. The defined binding complexes were reacted with colicin E3 and the reaction was assessed by the ability of the complexes to proceed with polypeptide synthesis. The results indicated that only one of the four complexes tested was completely resistant to colicin E3-induced inactivation: that of Phe-tRNA bound in the presence of poly(U) to the A-site. The poly(U) directed complex of AcPhe-tRNA and the T4-mRNA-directed complex at the A-site appeared slightly resistant, while the T4 mRNA initiation complex was inactivated by colicin E3 in a manner similar to non-complexed ribosomes. Colicin E3 added to ribosomes after protein synthesis had been initiated affected the subsequent polymerization in a manner corresponding to the response of the binding complexes. Thus, poly(U)-translating ribosomes were less affected than ribosomes translating the viral mRNA. The vulnerability of natural-mRNA-directed binding complexes to inactivation by colicin E3 is in accord with the mode of inactivation by the colicin in vivo.", "contents": "The effect of tRNA derivatives bound with natural or synthetic mRNA on the interaction of Escherichia coli ribosomes with colicin E3. Ribosomal binding complexes directed by poly(U) or T4 mRNA were formed with aminoacyl-tRNA or its derivatives bound to predominantly the P or A binding site. The defined binding complexes were reacted with colicin E3 and the reaction was assessed by the ability of the complexes to proceed with polypeptide synthesis. The results indicated that only one of the four complexes tested was completely resistant to colicin E3-induced inactivation: that of Phe-tRNA bound in the presence of poly(U) to the A-site. The poly(U) directed complex of AcPhe-tRNA and the T4-mRNA-directed complex at the A-site appeared slightly resistant, while the T4 mRNA initiation complex was inactivated by colicin E3 in a manner similar to non-complexed ribosomes. Colicin E3 added to ribosomes after protein synthesis had been initiated affected the subsequent polymerization in a manner corresponding to the response of the binding complexes. Thus, poly(U)-translating ribosomes were less affected than ribosomes translating the viral mRNA. The vulnerability of natural-mRNA-directed binding complexes to inactivation by colicin E3 is in accord with the mode of inactivation by the colicin in vivo."}
{"id": "PMID:1095373", "title": "Comparative effect of heparin on RNA synthesis of isolated rat-liver nucleoli and purified RNA polymerase A.", "content": "The polyanion heparin has been employed to study the interaction of rat liver DNA-dependent RNA polymerase A and its template under various conditions. Heparin very efficiently inhibits polymerase molecules, which are not bound to DNA or are associated with the template in a loose, i.e. non-specific fashion. Purified nucleoli, isloated from rat liver nuclei, contain RNA polymerase A in abundant quantities of which only a portion is bound in a transcriptional complex. Excess enzyme, which is contained in the nucleolus in a quasi free form, can be transferred to an exogenously added template and can be completely inhibited by the prior addition of heparin. The enzyme contained in a transcriptional complex, however, initiated in vivo and completing these RNA chains in vitro, is fully resistant to heparin. In contrast to these results it has been found that RNA polymerase A extracted from nuclei and purified by various chromatographic steps does not form heparin-resistant complexes, even after the enzyme has been bound to the DNA template. Moreover it has been found that purified RNA polymerase A transcribes truly native DNA extremely poorly, indicating that the enzyme is highly deficient in the act of initiation on duplex DNA. It is therefore questionable whether the interaction of the purified enzyme and isolated DNA represents binding to true initiation complexes as is observed in the intact nucleolus.", "contents": "Comparative effect of heparin on RNA synthesis of isolated rat-liver nucleoli and purified RNA polymerase A. The polyanion heparin has been employed to study the interaction of rat liver DNA-dependent RNA polymerase A and its template under various conditions. Heparin very efficiently inhibits polymerase molecules, which are not bound to DNA or are associated with the template in a loose, i.e. non-specific fashion. Purified nucleoli, isloated from rat liver nuclei, contain RNA polymerase A in abundant quantities of which only a portion is bound in a transcriptional complex. Excess enzyme, which is contained in the nucleolus in a quasi free form, can be transferred to an exogenously added template and can be completely inhibited by the prior addition of heparin. The enzyme contained in a transcriptional complex, however, initiated in vivo and completing these RNA chains in vitro, is fully resistant to heparin. In contrast to these results it has been found that RNA polymerase A extracted from nuclei and purified by various chromatographic steps does not form heparin-resistant complexes, even after the enzyme has been bound to the DNA template. Moreover it has been found that purified RNA polymerase A transcribes truly native DNA extremely poorly, indicating that the enzyme is highly deficient in the act of initiation on duplex DNA. It is therefore questionable whether the interaction of the purified enzyme and isolated DNA represents binding to true initiation complexes as is observed in the intact nucleolus."}
{"id": "PMID:1095374", "title": "Madopar versus sinemet. A clinical study on their effectiveness.", "content": "Fifteen well-documented Parkinson patients treated with Madopar were switched to Sinemet for 3 months. The results were measured with an objective test procedure and compared. The effects of Madopar and Sinemet were equal provided th amount of levodopa was the same. There was one drop-out, because of ventricular extrasystoles. Attention is paid to the occurrence of involuntary hyperkinesias and the on-off-effect.", "contents": "Madopar versus sinemet. A clinical study on their effectiveness. Fifteen well-documented Parkinson patients treated with Madopar were switched to Sinemet for 3 months. The results were measured with an objective test procedure and compared. The effects of Madopar and Sinemet were equal provided th amount of levodopa was the same. There was one drop-out, because of ventricular extrasystoles. Attention is paid to the occurrence of involuntary hyperkinesias and the on-off-effect."}
{"id": "PMID:1095375", "title": "Gaseous persufflation during machine perfusion of human kidneys before transplantation.", "content": "Massive air embolisation (persufflation) of four machine-perfused human kidneys occurred during preservation. This lasted for 60-120 min and caused increasing perfusion resistance. Pressure and flow returned to normal within 2 h after reperfusion. All kidneys showed immediate function after transplantation. Experiments with dog kidneys revealed closure of cortical vessels and increased kidney temperature during persufflation. Cortical flow became normal after 1 h of reperfusion.", "contents": "Gaseous persufflation during machine perfusion of human kidneys before transplantation. Massive air embolisation (persufflation) of four machine-perfused human kidneys occurred during preservation. This lasted for 60-120 min and caused increasing perfusion resistance. Pressure and flow returned to normal within 2 h after reperfusion. All kidneys showed immediate function after transplantation. Experiments with dog kidneys revealed closure of cortical vessels and increased kidney temperature during persufflation. Cortical flow became normal after 1 h of reperfusion."}
{"id": "PMID:1095378", "title": "Hemopoietic colony forming units in fresh and cryopreserved peripheral blood cells of canines and man.", "content": "Colony forming units (CFUa) were assayed in the peripheral blood and separated mononuclear cells of canines and man.  Fresh and cryopreserved samples were studied.  By plating 3 x 10-5 canine buffy coat cells or 3 x 10-6 human buffy coat cells between 33 plus or minus 6.6 and 38 plus or minus 1.0 colonies were observed.  A high degree of reproducibility was shown for duplicate plates and on repeated testing.  Cryopreservation for a one-month period resulted in a minimal recovery of 80 per cent CFUa for canine cells and 96 per cent for human cells.  In vivo correlation between peripheral blood CFUa and marrow repopulation was assessed indogs following supralethal whole body irradiation.  Prompt repopulation of the marrow was observed and this correlated well with the CFUa assay following infusion of stored buffy coat cells.  It was concluded that the peripheral blood of both man and dog have significant numbers of cells with CFUa capabilities that may be potentially useful for marrow grafting purposes.", "contents": "Hemopoietic colony forming units in fresh and cryopreserved peripheral blood cells of canines and man. Colony forming units (CFUa) were assayed in the peripheral blood and separated mononuclear cells of canines and man.  Fresh and cryopreserved samples were studied.  By plating 3 x 10-5 canine buffy coat cells or 3 x 10-6 human buffy coat cells between 33 plus or minus 6.6 and 38 plus or minus 1.0 colonies were observed.  A high degree of reproducibility was shown for duplicate plates and on repeated testing.  Cryopreservation for a one-month period resulted in a minimal recovery of 80 per cent CFUa for canine cells and 96 per cent for human cells.  In vivo correlation between peripheral blood CFUa and marrow repopulation was assessed indogs following supralethal whole body irradiation.  Prompt repopulation of the marrow was observed and this correlated well with the CFUa assay following infusion of stored buffy coat cells.  It was concluded that the peripheral blood of both man and dog have significant numbers of cells with CFUa capabilities that may be potentially useful for marrow grafting purposes."}
{"id": "PMID:1095379", "title": "Factors which influence development of macrophage-layer and spleen colonies in mice.", "content": "Irradiated mice infused with bone marrow cells developed colonies both in the spleen and on the macrophage layer of the cellulose acetate membrane which had been inserted into their peritoneal cavity. Factors influencing the ratio of spleen to macrophage-layer colonies were examined. The ratio of the colonies depended on the condition of the recipients rather than the condition of the injected cells provided there was no marked histoincompatibility between donors and recipients.", "contents": "Factors which influence development of macrophage-layer and spleen colonies in mice. Irradiated mice infused with bone marrow cells developed colonies both in the spleen and on the macrophage layer of the cellulose acetate membrane which had been inserted into their peritoneal cavity. Factors influencing the ratio of spleen to macrophage-layer colonies were examined. The ratio of the colonies depended on the condition of the recipients rather than the condition of the injected cells provided there was no marked histoincompatibility between donors and recipients."}
{"id": "PMID:1095380", "title": "Treatment of canine malignancies by 1200 R total body irradiation and autologous marrow grafts.", "content": "Twenty-five dogs with malignant lymphoma (L) and 18 dogs with solid, nonhematologic tumors (ST) were treated with 1200 R total body irradiation (TBI). Rescue from the otherwise lethal hemopoietic toxicity by infusion of autologous marrow aspirated before TBI was attempted, and survival, response to TBI, and immune reactivity post-grafting were determined. Eight L dogs survived more than 14 days post TBI and marrow grafting, and 12 out of 19 evaluable dogs showed a decrease of 75 per cent or more in clinically detectable tumor. There was no evident relationship between clinical status or marrow status before TBI and survival of more than 14 days or tumor response to TBI. Seven of the 8 survivors ultimately developed recurrent tumor. Eight ST dogs survived more than 14 days. Only 4 of 14 evaluable ST dogs showed significant clinical response of their tumor to TBI. Humoral and cellular immune reactivity were significantly impaired during the 10-week period following TBI and marrow grafting in all dogs studied. These results indicate that therapy in addition to lethal doses of TBI is necessary to cure spontaneous L or to significantly affect ST in dogs. They also provide baseline data which are necessary to assess the immunotherapeutic effectiveness of allogeneic marrow grafts.", "contents": "Treatment of canine malignancies by 1200 R total body irradiation and autologous marrow grafts. Twenty-five dogs with malignant lymphoma (L) and 18 dogs with solid, nonhematologic tumors (ST) were treated with 1200 R total body irradiation (TBI). Rescue from the otherwise lethal hemopoietic toxicity by infusion of autologous marrow aspirated before TBI was attempted, and survival, response to TBI, and immune reactivity post-grafting were determined. Eight L dogs survived more than 14 days post TBI and marrow grafting, and 12 out of 19 evaluable dogs showed a decrease of 75 per cent or more in clinically detectable tumor. There was no evident relationship between clinical status or marrow status before TBI and survival of more than 14 days or tumor response to TBI. Seven of the 8 survivors ultimately developed recurrent tumor. Eight ST dogs survived more than 14 days. Only 4 of 14 evaluable ST dogs showed significant clinical response of their tumor to TBI. Humoral and cellular immune reactivity were significantly impaired during the 10-week period following TBI and marrow grafting in all dogs studied. These results indicate that therapy in addition to lethal doses of TBI is necessary to cure spontaneous L or to significantly affect ST in dogs. They also provide baseline data which are necessary to assess the immunotherapeutic effectiveness of allogeneic marrow grafts."}
{"id": "PMID:1095381", "title": "Bone marrow regeneration after local injury: a review.", "content": "This paper is focused on a mechanically depleted medullary cavity as an experimental model for analysis of marrow regenerative programs. The reconstitution of marrow in an evacuated cavity is basically a local phenomenon in respect to the stimulus for regeneration and the origin of the responsible cells. The nature of the triggering stimulus is unknown, but it is probably related to disruption of the continuity of the marrow stroma and endosteum. The initiating cells appear to be independent lines of mesenchymal and hematopoietic stem cells bound to bone, most likely within the endosteum and haversian system. The mesenchymal cells form the characteristic marrow stroma. Hemic cell regeneration can occur without immigrant hematopoietic stem cells, although such cells are known to contribute to later stages of repopulation. The formation and resorption of trabecular bone appears to be intimately related to the development of a sinusoidal matrix, perhaps by serving as a callus or supporting lattice and perhaps by providing a mechanism for distribution of stromal progenitors. Hematopoiesis is initiated in sites of active bone resorptive. The interplay of events consequent to marrow removal is strikingly similar to that seen with heterotopic marrow implants. Because stromal stem cells, unlike hematopoietic stem cells, do not migrate from distant sites, marrow stroma is the limiting factor in recovery from localized injury. Stromal stem cells are fairly radiosensitive but are not as sensitive as hematopoietic stem cells. The apparent radioresistance of stromal elements in an intact marrow seems to be due to their very low turnover rate. Latent radiation damage can be readily unmasked by conditions that promote their proliferation. This no doubt accounts for the radiosensitivity of stroma in an evacuated femur or heterotopic implant in contrast to its continued functional integrity with similar irradiation of in situ marrow. Even in an intact marrow, however, exposures in the 1000 rad range can lead to slowly evolving hypocellularity associated with diminished blood flow. With higher doses, aplasia of the irradiated site becomes progressively more generalized. It remains to be seen whether this limiting condition is due to the loss of specific regulatory functions or stromal components or merely reflects sinusoidal damage.", "contents": "Bone marrow regeneration after local injury: a review. This paper is focused on a mechanically depleted medullary cavity as an experimental model for analysis of marrow regenerative programs. The reconstitution of marrow in an evacuated cavity is basically a local phenomenon in respect to the stimulus for regeneration and the origin of the responsible cells. The nature of the triggering stimulus is unknown, but it is probably related to disruption of the continuity of the marrow stroma and endosteum. The initiating cells appear to be independent lines of mesenchymal and hematopoietic stem cells bound to bone, most likely within the endosteum and haversian system. The mesenchymal cells form the characteristic marrow stroma. Hemic cell regeneration can occur without immigrant hematopoietic stem cells, although such cells are known to contribute to later stages of repopulation. The formation and resorption of trabecular bone appears to be intimately related to the development of a sinusoidal matrix, perhaps by serving as a callus or supporting lattice and perhaps by providing a mechanism for distribution of stromal progenitors. Hematopoiesis is initiated in sites of active bone resorptive. The interplay of events consequent to marrow removal is strikingly similar to that seen with heterotopic marrow implants. Because stromal stem cells, unlike hematopoietic stem cells, do not migrate from distant sites, marrow stroma is the limiting factor in recovery from localized injury. Stromal stem cells are fairly radiosensitive but are not as sensitive as hematopoietic stem cells. The apparent radioresistance of stromal elements in an intact marrow seems to be due to their very low turnover rate. Latent radiation damage can be readily unmasked by conditions that promote their proliferation. This no doubt accounts for the radiosensitivity of stroma in an evacuated femur or heterotopic implant in contrast to its continued functional integrity with similar irradiation of in situ marrow. Even in an intact marrow, however, exposures in the 1000 rad range can lead to slowly evolving hypocellularity associated with diminished blood flow. With higher doses, aplasia of the irradiated site becomes progressively more generalized. It remains to be seen whether this limiting condition is due to the loss of specific regulatory functions or stromal components or merely reflects sinusoidal damage."}
{"id": "PMID:1095384", "title": "Augmentation of marrow growth by thymocytes separated by discontinuous albumin density-gradient centrifugation.", "content": "Mouse thymocytes were separated by discontinuous albumin density-gradient centrifugation. The ability of B6 thymocytes from the gradient fractions to increase the number of spleen nodules formed by B6 marrow cells in B6D2F1 recipients was compared with that of similar numbers of B6 thymocytes from an unfractionated cell suspension. Thymocytes from any of the gradient fractions were not more effective than thymocytes from an unfractionated suspension in augmenting marrow-cell growth, indicating that the presumptive 'effective cell type' in this system cannot be separated from the total thymocyte population by its buoyant density properties.", "contents": "Augmentation of marrow growth by thymocytes separated by discontinuous albumin density-gradient centrifugation. Mouse thymocytes were separated by discontinuous albumin density-gradient centrifugation. The ability of B6 thymocytes from the gradient fractions to increase the number of spleen nodules formed by B6 marrow cells in B6D2F1 recipients was compared with that of similar numbers of B6 thymocytes from an unfractionated cell suspension. Thymocytes from any of the gradient fractions were not more effective than thymocytes from an unfractionated suspension in augmenting marrow-cell growth, indicating that the presumptive 'effective cell type' in this system cannot be separated from the total thymocyte population by its buoyant density properties."}
{"id": "PMID:1095390", "title": "Inhibitors of the adhesiveness of enteropathogenic E. coli.", "content": "The entero-pathogenic strain of E. coli 0125: K 70 was able to adhere to washed guinea-pig erythrocytes and to cause their agglutination. Electron microscopy revealed this strain to be rich in fimbriae. They were able to cause hemagglutination by themselves, generally at protein concentration of 10 to 100 mug per ml. D-mannose and alpha-methylmannoside were able to inhibit hemagglutination by the whole bacteria or their isolated fimbriae at concentrations of 0.003 to 0.012 mg/ml. L-mannose, D-lactose, D-glucose, N-acetylglucosamine, D-galactose, N-acetyl-D-galactosamine, D-fucose, did not exert any inhibiting effect at concentrations as high as 50 mg/ml. N-acetyl-neuraminic acid was also uneffective at concentration as high as 9 mg/ml.", "contents": "Inhibitors of the adhesiveness of enteropathogenic E. coli. The entero-pathogenic strain of E. coli 0125: K 70 was able to adhere to washed guinea-pig erythrocytes and to cause their agglutination. Electron microscopy revealed this strain to be rich in fimbriae. They were able to cause hemagglutination by themselves, generally at protein concentration of 10 to 100 mug per ml. D-mannose and alpha-methylmannoside were able to inhibit hemagglutination by the whole bacteria or their isolated fimbriae at concentrations of 0.003 to 0.012 mg/ml. L-mannose, D-lactose, D-glucose, N-acetylglucosamine, D-galactose, N-acetyl-D-galactosamine, D-fucose, did not exert any inhibiting effect at concentrations as high as 50 mg/ml. N-acetyl-neuraminic acid was also uneffective at concentration as high as 9 mg/ml."}
{"id": "PMID:1095393", "title": "[B-lymphocytes subpopulation. Rapid identification with a histoimmunological method (author's transl)].", "content": "After extraction of tissues (spleen, thymus, blood) B cell bearing surface immunoglobulins were specifically labelled with Fab-peroxidase conjugate in light and electron microscopy. Positive and negative labelled lymphocyte were counted. This procedure allowed us to quantitate B and T cell in different diseases in peripheral blood and within lesions. It may represent a new immunopathological approach.", "contents": "[B-lymphocytes subpopulation. Rapid identification with a histoimmunological method (author's transl)]. After extraction of tissues (spleen, thymus, blood) B cell bearing surface immunoglobulins were specifically labelled with Fab-peroxidase conjugate in light and electron microscopy. Positive and negative labelled lymphocyte were counted. This procedure allowed us to quantitate B and T cell in different diseases in peripheral blood and within lesions. It may represent a new immunopathological approach."}
{"id": "PMID:1095395", "title": "Effect of excision repair system on antibacterial and mutagenic activity of daunomycin and other intercalating agents in Salmonella typhimurium.", "content": "Daunomycin and adriamycin, are more mutagenic and antibacterial for a strain of Salmonella typhimurium defective for the uvrB gene than for its uvr+ counterpart. Other intercalating agents, as some acridine dyes, affect equally the two bacterial strains.", "contents": "Effect of excision repair system on antibacterial and mutagenic activity of daunomycin and other intercalating agents in Salmonella typhimurium. Daunomycin and adriamycin, are more mutagenic and antibacterial for a strain of Salmonella typhimurium defective for the uvrB gene than for its uvr+ counterpart. Other intercalating agents, as some acridine dyes, affect equally the two bacterial strains."}
{"id": "PMID:1095396", "title": "Does pentagastrin supress secretin induced pepsin secretion in Heidenhain pouch dogs?", "content": "Secretin-stimulated pepsin secretion from Heidenhain pouches was significantly depressed by concomitant pentagastrin. Pentagastrin by itself was without effect on pouch pepsin. Methacholine, on the other hand, did not antagonize secretin-stimulated pouch pepsin.", "contents": "Does pentagastrin supress secretin induced pepsin secretion in Heidenhain pouch dogs? Secretin-stimulated pepsin secretion from Heidenhain pouches was significantly depressed by concomitant pentagastrin. Pentagastrin by itself was without effect on pouch pepsin. Methacholine, on the other hand, did not antagonize secretin-stimulated pouch pepsin."}
{"id": "PMID:1095398", "title": "Are there somatostatin-containing nerves in the rat gut? Immunohistochemical evidence for a new type of peripheral nerves.", "content": "Antibodies to somatostatin, a recently isolated hypothalamic peptide inhibiting growth hormone release, were used in immunohistochemical studies on the gastrointestinal tract. Somatostatin containing cells in the stomach, and somatostatin-containing nerves in the small and large intestine, could be demonstrated. These findings give evidence of a new type of nerve in the gut.", "contents": "Are there somatostatin-containing nerves in the rat gut? Immunohistochemical evidence for a new type of peripheral nerves. Antibodies to somatostatin, a recently isolated hypothalamic peptide inhibiting growth hormone release, were used in immunohistochemical studies on the gastrointestinal tract. Somatostatin containing cells in the stomach, and somatostatin-containing nerves in the small and large intestine, could be demonstrated. These findings give evidence of a new type of nerve in the gut."}
{"id": "PMID:1095399", "title": "U.S. organized family planning programs in FY 1974.", "content": "Most organized family planning programs combine the delivery of contraceptive services with a wide range of preventive health services. An estimated 3.3 million women, 97 percent of all who received family planning services in organized programs in FY 1974, received one or more medical services. At least 2.7 mission women received each of the 'core' medical services recommended by DHEW -- pap smear, blood pressure and pelvic and breast examinations. About 2.4 million women were tested for VD; 2.1 million women received urinalyses and 1.9 million received blood tests. Other services provided included pregnancy testing, infertility workup, and rubella and sickle cell screening.", "contents": "U.S. organized family planning programs in FY 1974. Most organized family planning programs combine the delivery of contraceptive services with a wide range of preventive health services. An estimated 3.3 million women, 97 percent of all who received family planning services in organized programs in FY 1974, received one or more medical services. At least 2.7 mission women received each of the 'core' medical services recommended by DHEW -- pap smear, blood pressure and pelvic and breast examinations. About 2.4 million women were tested for VD; 2.1 million women received urinalyses and 1.9 million received blood tests. Other services provided included pregnancy testing, infertility workup, and rubella and sickle cell screening."}
{"id": "PMID:1095438", "title": "Calcium antagonists and islet function. I. Inhibition of insulin release by verapamil.", "content": "Verapamil is a potent calcium antagonist known to inhibit excitation-contraction coupling in both myocardium and myometrium. Its effect upon glucose- and sulfonylurea-induced insulin release was investigated in the isolated perfused rat pancreas. After twenty-five minutes' pretreatment and at concentrations ranging between 0.8 and 8.1 muM, verapamil caused a dose-related inhibition of glucose-induced insulin release during both the early and late phase of the secretory process. At a concentration of 0.8 muM, the degree of inhibition was more marked when the exposure time to verapamil prior to stimulation with glucose was increased to sixty minutes. Verapamil also inhibited gliclazide-induced insulin release. Infusion of verapamil during the late phase of the secretory response to glucose demonstrated that the inhibition of insulin release was an immediate and reversible phenomenon. The inhibitory effect of verapamil was enhanced at a subnormal calcium concentration and reduced at a high calcium concentration. These findings are consistent with the well-known calcium dependency of both glucose- and sulfonylurea-induced insulin release and suggest that verapamil might be a promising tool for further studies on the interactions between cations and secretagogues in the beta-cell secretory process.", "contents": "Calcium antagonists and islet function. I. Inhibition of insulin release by verapamil. Verapamil is a potent calcium antagonist known to inhibit excitation-contraction coupling in both myocardium and myometrium. Its effect upon glucose- and sulfonylurea-induced insulin release was investigated in the isolated perfused rat pancreas. After twenty-five minutes' pretreatment and at concentrations ranging between 0.8 and 8.1 muM, verapamil caused a dose-related inhibition of glucose-induced insulin release during both the early and late phase of the secretory process. At a concentration of 0.8 muM, the degree of inhibition was more marked when the exposure time to verapamil prior to stimulation with glucose was increased to sixty minutes. Verapamil also inhibited gliclazide-induced insulin release. Infusion of verapamil during the late phase of the secretory response to glucose demonstrated that the inhibition of insulin release was an immediate and reversible phenomenon. The inhibitory effect of verapamil was enhanced at a subnormal calcium concentration and reduced at a high calcium concentration. These findings are consistent with the well-known calcium dependency of both glucose- and sulfonylurea-induced insulin release and suggest that verapamil might be a promising tool for further studies on the interactions between cations and secretagogues in the beta-cell secretory process."}
{"id": "PMID:1095439", "title": "Basal and postprotein insulin and glucagon levels during a high and low carbohydrate intake and their relationships to plasma triglycerides.", "content": "The effects of acute and chronic differences in the carbohydrate content of the diet on plasma insulin, glucagon, insulin-glucagon molar ratio (I/G), and triglycerides were studied. Acute effects were studied by varying the carbohydrate content of a single test meal, while chronic effects were determined by varying the carbohydrate content of the diet for a week. A test meal containing 0.6 gm of gelatin per kilogram plus 0.6 gm. per kilogram of glucose resulted in much higher levels of insulin and I/G (p smaller than 0.005), lower glucagon levels (p smaller than 0.05), and slightly higher triglycerides (N.S.) than did a meal of 1.2 gm, per kilogram of gelatin alone. One week of a 12 gm. carbohydrate, 2870-calorie diet lowered insulin (p smaller than 0.001), I/G (p smaller than 0.05), and triglycerides (p smaller than 0.001) and increased glucagon (N.S.), whereas a 390-gm. carbohydrate, 2784-calorie intake significantly increased insulin, I/G, and triglycerides (p smaller than 0.005) and lowered glucagon (p smaller than 0.02) within two days; even greater changes in hormones were observed on a 510-gm. carbohydrate intake. Of those patients in whom a high carbohydrate intake induced a triglyceride rise of at least 40 mg. per deciliter, a significant correlation between the change in I/G and the change in triglycerides was noted (r equals 0.85; p smaller than 0.01). The results are compatible with but do not prove the proposal that pancreatic alpha and beta cells play a mediating role in carbohydrate induction of hyperlipidemia.", "contents": "Basal and postprotein insulin and glucagon levels during a high and low carbohydrate intake and their relationships to plasma triglycerides. The effects of acute and chronic differences in the carbohydrate content of the diet on plasma insulin, glucagon, insulin-glucagon molar ratio (I/G), and triglycerides were studied. Acute effects were studied by varying the carbohydrate content of a single test meal, while chronic effects were determined by varying the carbohydrate content of the diet for a week. A test meal containing 0.6 gm of gelatin per kilogram plus 0.6 gm. per kilogram of glucose resulted in much higher levels of insulin and I/G (p smaller than 0.005), lower glucagon levels (p smaller than 0.05), and slightly higher triglycerides (N.S.) than did a meal of 1.2 gm, per kilogram of gelatin alone. One week of a 12 gm. carbohydrate, 2870-calorie diet lowered insulin (p smaller than 0.001), I/G (p smaller than 0.05), and triglycerides (p smaller than 0.001) and increased glucagon (N.S.), whereas a 390-gm. carbohydrate, 2784-calorie intake significantly increased insulin, I/G, and triglycerides (p smaller than 0.005) and lowered glucagon (p smaller than 0.02) within two days; even greater changes in hormones were observed on a 510-gm. carbohydrate intake. Of those patients in whom a high carbohydrate intake induced a triglyceride rise of at least 40 mg. per deciliter, a significant correlation between the change in I/G and the change in triglycerides was noted (r equals 0.85; p smaller than 0.01). The results are compatible with but do not prove the proposal that pancreatic alpha and beta cells play a mediating role in carbohydrate induction of hyperlipidemia."}
{"id": "PMID:1095440", "title": "Paradoxical glucagon response after stimulation with glucose and arginine in isolated pancreatic sand rat islets.", "content": "Isolated pancreatic islets of normoglycemic sand rats do not respond to 2.5 mM glucose with an enhanced glucagon secretion, which could be observed in normal Wistar rats. Arginine stimulates glucagon release in the presence of 2.5 mM glucose in Wistar rats as well as in sand rats. The secretion pattern is not caused by insulin deficiency since sand rat islets are characterized by an increased insulin secretion rate in vitro. This paradoxical glucagon secretion is not caused by a changed glucagon content but might be related to this species which is able to develop a diabetic syndrome spontaneously.", "contents": "Paradoxical glucagon response after stimulation with glucose and arginine in isolated pancreatic sand rat islets. Isolated pancreatic islets of normoglycemic sand rats do not respond to 2.5 mM glucose with an enhanced glucagon secretion, which could be observed in normal Wistar rats. Arginine stimulates glucagon release in the presence of 2.5 mM glucose in Wistar rats as well as in sand rats. The secretion pattern is not caused by insulin deficiency since sand rat islets are characterized by an increased insulin secretion rate in vitro. This paradoxical glucagon secretion is not caused by a changed glucagon content but might be related to this species which is able to develop a diabetic syndrome spontaneously."}
{"id": "PMID:1095441", "title": "An apparent abnormaltiy of the B-cell microtubular system in spiny mice (Acomys cahirinus).", "content": "The pancreatic B-cell contains microtubules, which are thought to participate in the process of insulin release. In order to disclose a possible abnormaltiy of this B-cell microtubular system in animals with islet dysfunction, isolated islets from normal rats and mice, as well as from diabetic mutant mice (DBM mice) and from spiny mice (Acomys cahirinus) were incubated in the presence of vincristine, which causes the precipitation of the microtubular protein into paracrystalline deposits. Ultrastructural examination of the islets indicated that the volume-density of vincristine-induced deposits was markedly reduced in B-cells of spiny mice, when compared to that found in normal rats and mice and DBM mice. Exposure of the islets from spiny mice to a high glucose concentration, concomittantly to vincristine, caused a further reduction in vincristine-induced crystals content of the B-cell. It is speculated that an impairment of the B-cell microtubular system may account for the deficiency of insulin release found in spiny mice.", "contents": "An apparent abnormaltiy of the B-cell microtubular system in spiny mice (Acomys cahirinus). The pancreatic B-cell contains microtubules, which are thought to participate in the process of insulin release. In order to disclose a possible abnormaltiy of this B-cell microtubular system in animals with islet dysfunction, isolated islets from normal rats and mice, as well as from diabetic mutant mice (DBM mice) and from spiny mice (Acomys cahirinus) were incubated in the presence of vincristine, which causes the precipitation of the microtubular protein into paracrystalline deposits. Ultrastructural examination of the islets indicated that the volume-density of vincristine-induced deposits was markedly reduced in B-cells of spiny mice, when compared to that found in normal rats and mice and DBM mice. Exposure of the islets from spiny mice to a high glucose concentration, concomittantly to vincristine, caused a further reduction in vincristine-induced crystals content of the B-cell. It is speculated that an impairment of the B-cell microtubular system may account for the deficiency of insulin release found in spiny mice."}
{"id": "PMID:1095443", "title": "[Carcinoma of the uterine cervix and herpes simplex virus type 2. Investigations on the causal relationship (author's transl)].", "content": "Epidemiological serological studies have shown that women with carcinoma of the uterine cervix show a higher incidence of Herpes Simplex Virus Type 2 (HSV 2) than controls. The attempt to demonstrate the genomic DNS of the HSV 2 in cells of carcinoma of the cervix by experiments of hybridization is reported. The investigations were carried out directly on material from carcinoma of the cervix and also on cell cultures from tumor material. The majority of the cultures grew fibroblasts but some cultures grew cells with the characteristics of malignant cells. The hybridization of DNS from portions of carcinoma or of DNS from cell cultures with complementary HSV 2 radioactive RNS showed in no case HSV-2-DNS in a quantity of one or one half genomic equivalent per cell. These investigations suggest that a casual relationship between carcinoma of the uterine cervix and an infection with HSV 2 is unlikely. However, it cannot be excluded that carcinoma of the uterine cervix has different quantitative proportions than the Burkitt-lymphoma and that only a fraction of the genomic material of the virus (smaller than 1/10) is incorporated into the malignant cell.", "contents": "[Carcinoma of the uterine cervix and herpes simplex virus type 2. Investigations on the causal relationship (author's transl)]. Epidemiological serological studies have shown that women with carcinoma of the uterine cervix show a higher incidence of Herpes Simplex Virus Type 2 (HSV 2) than controls. The attempt to demonstrate the genomic DNS of the HSV 2 in cells of carcinoma of the cervix by experiments of hybridization is reported. The investigations were carried out directly on material from carcinoma of the cervix and also on cell cultures from tumor material. The majority of the cultures grew fibroblasts but some cultures grew cells with the characteristics of malignant cells. The hybridization of DNS from portions of carcinoma or of DNS from cell cultures with complementary HSV 2 radioactive RNS showed in no case HSV-2-DNS in a quantity of one or one half genomic equivalent per cell. These investigations suggest that a casual relationship between carcinoma of the uterine cervix and an infection with HSV 2 is unlikely. However, it cannot be excluded that carcinoma of the uterine cervix has different quantitative proportions than the Burkitt-lymphoma and that only a fraction of the genomic material of the virus (smaller than 1/10) is incorporated into the malignant cell."}
{"id": "PMID:1095445", "title": "Age-related changes in the bone marrow and spleen of SAS/4 mice.", "content": "The total number of nucleated cells in the bone marrow of SAS/4 mice increase some twofold between 1 and 24 months of age but when related to body weight remains essentially constant over a wide range of ages. The concentration of CFU-S in femoral marrow is also constant with age and since other bones containing marrow appear, at least in young mice, to have the same CFU-S concentration as the femur it is concluded that the CFU-S compartment size of the whole bone marrow is independent of age when expressed on a body weight basis, In contrast, both the absolute number and the concentration of exogenous CFU-S in the spleen decline markedly in old mice. Smilary there is a decline in the number of endogenous colony-forming cells and the spleens of 24-month-old mice seem virtually devoid of such colonies. Not only were older mice less capable of supporting the growth of endogenous colonies, but their spleens also appear to provide a poorer environment for exogenous colony growth when compared with growth in younger recipient spleens.", "contents": "Age-related changes in the bone marrow and spleen of SAS/4 mice. The total number of nucleated cells in the bone marrow of SAS/4 mice increase some twofold between 1 and 24 months of age but when related to body weight remains essentially constant over a wide range of ages. The concentration of CFU-S in femoral marrow is also constant with age and since other bones containing marrow appear, at least in young mice, to have the same CFU-S concentration as the femur it is concluded that the CFU-S compartment size of the whole bone marrow is independent of age when expressed on a body weight basis, In contrast, both the absolute number and the concentration of exogenous CFU-S in the spleen decline markedly in old mice. Smilary there is a decline in the number of endogenous colony-forming cells and the spleens of 24-month-old mice seem virtually devoid of such colonies. Not only were older mice less capable of supporting the growth of endogenous colonies, but their spleens also appear to provide a poorer environment for exogenous colony growth when compared with growth in younger recipient spleens."}
{"id": "PMID:1095452", "title": "Serum antibodies in human pancreatic disease.", "content": "An immunofluorescent study of sera from patients with various pancreatic diseases and from controls was carried out using human pancreas as substrate. A reproducible pattern of immunofluorscence, consisting of a coarse granular appearance in the cytoplasm of acinar cells of the pancreas and independent of the ABO blood group system was found. This occurred in 21 of 61 patients with acute pancreatitis; it was not found in other pancreatic diseases, and occurred in only four of 170 control subjects consisting of mixed hospital patients and healthy adults. Preliminary absorption experiments suggest that the  antigen is located in the microsomal fraction of pancreatic homogenates and may be organ-specific. The relationship of positive antibody tests to aetiological factors is discussed.", "contents": "Serum antibodies in human pancreatic disease. An immunofluorescent study of sera from patients with various pancreatic diseases and from controls was carried out using human pancreas as substrate. A reproducible pattern of immunofluorscence, consisting of a coarse granular appearance in the cytoplasm of acinar cells of the pancreas and independent of the ABO blood group system was found. This occurred in 21 of 61 patients with acute pancreatitis; it was not found in other pancreatic diseases, and occurred in only four of 170 control subjects consisting of mixed hospital patients and healthy adults. Preliminary absorption experiments suggest that the  antigen is located in the microsomal fraction of pancreatic homogenates and may be organ-specific. The relationship of positive antibody tests to aetiological factors is discussed."}
{"id": "PMID:1095460", "title": "The promoters and bidirectional transcription in the immC region of P 22 and L phages.", "content": "It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5.", "contents": "The promoters and bidirectional transcription in the immC region of P 22 and L phages. It is proposed that the transcription of the c-2 repressor gene initiates at two promoters, pre and prm, similarly to the transcription of the c-I gene of lambda phage. In the P22 phage, these promoters are defined by mutations of c54 and K5."}
{"id": "PMID:1095461", "title": "Penicillinamidohydrolase in Escherichia coli. I. Substrate specificity.", "content": "Substrate specificity of the bacterial penicillinamidohydrolase (penicillinacylase, EC 3.5.1.11) from Escherichia coli was determined by measuring initial rates of enzyme hydrolysis of different substrates within zero order kinetics. Some N-phenylacetyl derivatives of amino acids and amides of phenylacetic acid and phenoxyacetic acid of different substituted amides of these acids or amides, structurally and chemically similar to these compounds, served as substrates. Significant differences in ratios of initial rates of the enzyme hydrolysis of different substrates were found using a toluenized suspension of bacterial cells or a crude enzyme preparation, in spite of the fact that the enzyme is localized between the cell wall and cytoplasmic membrane, in the so-called periplasmic space. N-phenylacetyl derivatives are the most rapidly hydrolyzed substrates. Beta-phenylpropionamide and 4-phenylbutyramide were not utilized as substrates. The substrate specificity of the enzyme is discussed with respect to a possible use of certain colourless compounds as substrates, hydrolysis of which yields chromophor products suitable for a simple and rapid assay of the enzyme activity.", "contents": "Penicillinamidohydrolase in Escherichia coli. I. Substrate specificity. Substrate specificity of the bacterial penicillinamidohydrolase (penicillinacylase, EC 3.5.1.11) from Escherichia coli was determined by measuring initial rates of enzyme hydrolysis of different substrates within zero order kinetics. Some N-phenylacetyl derivatives of amino acids and amides of phenylacetic acid and phenoxyacetic acid of different substituted amides of these acids or amides, structurally and chemically similar to these compounds, served as substrates. Significant differences in ratios of initial rates of the enzyme hydrolysis of different substrates were found using a toluenized suspension of bacterial cells or a crude enzyme preparation, in spite of the fact that the enzyme is localized between the cell wall and cytoplasmic membrane, in the so-called periplasmic space. N-phenylacetyl derivatives are the most rapidly hydrolyzed substrates. Beta-phenylpropionamide and 4-phenylbutyramide were not utilized as substrates. The substrate specificity of the enzyme is discussed with respect to a possible use of certain colourless compounds as substrates, hydrolysis of which yields chromophor products suitable for a simple and rapid assay of the enzyme activity."}
{"id": "PMID:1095462", "title": "Growth of Cryptococcus neoformans in UV-irradiated excreta of pigeons.", "content": "UV irradiation of pigeon droppings resulted in an increased concentration of some inhibitors (peroxides) of growth of Cryptococcus neofarmans. This may be, in addition to the direct germicidal action of sunshine, another cause of the rare occurrence of this fungus in pigeon droppings on unsheltered sites in natural habitats.", "contents": "Growth of Cryptococcus neoformans in UV-irradiated excreta of pigeons. UV irradiation of pigeon droppings resulted in an increased concentration of some inhibitors (peroxides) of growth of Cryptococcus neofarmans. This may be, in addition to the direct germicidal action of sunshine, another cause of the rare occurrence of this fungus in pigeon droppings on unsheltered sites in natural habitats."}
{"id": "PMID:1095463", "title": "The in vitro and in vivo effects of carrageenin on humoral and cellular factors of natural resistance.", "content": "The inhibitory effect of carrageenin, a sulfated algal polygalactose, on humoral factors of natural resistance is discussed. In dependence on dose and time, the influence of non-specific and specific bacteriolysis, on the in vitro and in vivo opsonic activity and on the course of infection was studied.", "contents": "The in vitro and in vivo effects of carrageenin on humoral and cellular factors of natural resistance. The inhibitory effect of carrageenin, a sulfated algal polygalactose, on humoral factors of natural resistance is discussed. In dependence on dose and time, the influence of non-specific and specific bacteriolysis, on the in vitro and in vivo opsonic activity and on the course of infection was studied."}
{"id": "PMID:1095464", "title": "Novel approaches to the mode of action of colicins.", "content": "According to the theory of Fredericq (1949) and Nomura (1964), colicins are attached by specific receptor sites in the cell walls of sensitive bacteria, which mediate their inhibitive effects. During last years, a great variety of experimental data have been accumulated, some of which cannot be easily interpreted in terms of this theory. There exist considerable discrepancies concerning the chemical nature and molecular weight of isolated receptors. The attachment of a colicin onto its receptor need not be irreversible. The inhibition of numerous membrane-associated functions in colicin-tolerant mutants suggests their pleiotropic deletion nature. The difference between colicin resistance and colicin tolerance does not seem to be clear-cut. Cells of stable L-forms of protoplast type, completely devoid of their walls, retain in most cases the same patterns of sensitivity to colicins as rods of the same strains. Experimental changes in the relationship between the cell wall and the cytoplasmic membrane decrease colicin sensitivity of the cells. Colicin E3 has been found to be a specific endoribonuclease, able to cleave a terminal fragment from the 16 S rRNA also in isolated ribosomes in vitro: not only in ribosomes from sensitive bacteria, but also in those from resistant ones and from eukaryotic cells. A destabilization of the DNA helix was induced by colicin E2 in vitro as in vivo. It seems that there exist two distinct types of colicin receptors with different functions: those in the cell wall, and those in the cytoplasmic membrane. Only the contact of colicins with the latter ones is biologically effective and starts both stages of their inhibitive effect: the reversible and the irreversible ones.", "contents": "Novel approaches to the mode of action of colicins. According to the theory of Fredericq (1949) and Nomura (1964), colicins are attached by specific receptor sites in the cell walls of sensitive bacteria, which mediate their inhibitive effects. During last years, a great variety of experimental data have been accumulated, some of which cannot be easily interpreted in terms of this theory. There exist considerable discrepancies concerning the chemical nature and molecular weight of isolated receptors. The attachment of a colicin onto its receptor need not be irreversible. The inhibition of numerous membrane-associated functions in colicin-tolerant mutants suggests their pleiotropic deletion nature. The difference between colicin resistance and colicin tolerance does not seem to be clear-cut. Cells of stable L-forms of protoplast type, completely devoid of their walls, retain in most cases the same patterns of sensitivity to colicins as rods of the same strains. Experimental changes in the relationship between the cell wall and the cytoplasmic membrane decrease colicin sensitivity of the cells. Colicin E3 has been found to be a specific endoribonuclease, able to cleave a terminal fragment from the 16 S rRNA also in isolated ribosomes in vitro: not only in ribosomes from sensitive bacteria, but also in those from resistant ones and from eukaryotic cells. A destabilization of the DNA helix was induced by colicin E2 in vitro as in vivo. It seems that there exist two distinct types of colicin receptors with different functions: those in the cell wall, and those in the cytoplasmic membrane. Only the contact of colicins with the latter ones is biologically effective and starts both stages of their inhibitive effect: the reversible and the irreversible ones."}
{"id": "PMID:1095466", "title": "Developing a utilization review model for community mental health centers.", "content": "During their struggles to develop a utilization review model for a community mental health center, members of an interdisciplinary committee identified several issues they believe are important in establishing such a model. They include comprehensiveness of participation and of areas for review (the review committee should represent all disciplines and programs, and should be concerned with any aspect of center functioning), a problem-review approach in which subcommittees carry out documented studies of issues or problems, and specific provision for feedback and implementation of the results.", "contents": "Developing a utilization review model for community mental health centers. During their struggles to develop a utilization review model for a community mental health center, members of an interdisciplinary committee identified several issues they believe are important in establishing such a model. They include comprehensiveness of participation and of areas for review (the review committee should represent all disciplines and programs, and should be concerned with any aspect of center functioning), a problem-review approach in which subcommittees carry out documented studies of issues or problems, and specific provision for feedback and implementation of the results."}
{"id": "PMID:1095472", "title": "Adsorption in vitro to Escherichia coli of antibodies and other proteins in bovine serum and colostrum and its effects on the production of E. coli agglutinins.", "content": "IgGl, IgG2, IgA and IgM from bovine serum and colostrum are adsorbed by Escherichia coli in vitro; lactoferrin is also adsorbed from colostrum and alpha2 macroglobulin from serum. The colostral adsorbed proteins on E. coli appear to reduce formation of agglutinins when the treated bacteria are injected into rabbits and guinea-pigs. Assay of the concentration of proteins dissociated from colostrum-treated cells showed equal amounts of secretory IgA AND IgGl, half that amount of IgG2, and traces of IgM and lactoferrin. Dissociation of proteins from serum-treated E. coli yielded equal amounts of IgGl and IgG2, traces of IgA and an alpha2 macroglobulin, but no IgM.", "contents": "Adsorption in vitro to Escherichia coli of antibodies and other proteins in bovine serum and colostrum and its effects on the production of E. coli agglutinins. IgGl, IgG2, IgA and IgM from bovine serum and colostrum are adsorbed by Escherichia coli in vitro; lactoferrin is also adsorbed from colostrum and alpha2 macroglobulin from serum. The colostral adsorbed proteins on E. coli appear to reduce formation of agglutinins when the treated bacteria are injected into rabbits and guinea-pigs. Assay of the concentration of proteins dissociated from colostrum-treated cells showed equal amounts of secretory IgA AND IgGl, half that amount of IgG2, and traces of IgM and lactoferrin. Dissociation of proteins from serum-treated E. coli yielded equal amounts of IgGl and IgG2, traces of IgA and an alpha2 macroglobulin, but no IgM."}
{"id": "PMID:1095473", "title": "Promotion of secondary anti-DNP antibody production in mice by type III pneumococcal polysaccharide (SIII) and dinitrophenylated rabbit antibody to SIII.", "content": "Type III pneumococcal polysaccharide (SIII) is able markedly to increase the adoptive IgG ANTI-DNP antibody response of B cells primed to DNP-flagellin and stimulated with DNP conjugated to the heterologous carrier, rabbit globulin, provided the latter has anti-SIII activity. The stimulatory effect is apparently accessory cell-dependent as well as being unequivocally T cell-dependent. Although no positive evidence is available, the possibility exists that non-specific T-cell activation is involved in the stimulating effect of anti-SIII plus SIII.", "contents": "Promotion of secondary anti-DNP antibody production in mice by type III pneumococcal polysaccharide (SIII) and dinitrophenylated rabbit antibody to SIII. Type III pneumococcal polysaccharide (SIII) is able markedly to increase the adoptive IgG ANTI-DNP antibody response of B cells primed to DNP-flagellin and stimulated with DNP conjugated to the heterologous carrier, rabbit globulin, provided the latter has anti-SIII activity. The stimulatory effect is apparently accessory cell-dependent as well as being unequivocally T cell-dependent. Although no positive evidence is available, the possibility exists that non-specific T-cell activation is involved in the stimulating effect of anti-SIII plus SIII."}
{"id": "PMID:1095474", "title": "Splenic mediated erythrocyte cytotoxicity in malaria.", "content": "Cell-mediated cytotoxicity in virulent rodent malaria has been demonstrated in vitro, whereby splenic cells effected specific lysis of 51Cr-labelled erythrocytes from parasitized animals. More than one cellular cytolytic effector system appeared to be operative in the mouse. One effector system involved splenic macrophages, from normal or immune animals, which were increasingly cytotoxic to target cells in the presence of antibody. A second effector system involved nylonpurified immune spleen cells which were significantly more cytotoxic than similary prepared normal spleen cells in the presence of immune serum. Although antibody alone was not cytolytic, the data strengthen the concept that immune spleen cells and antibody can interact in a co-operative fashion to mediate cytotoxic reactions in malaria.", "contents": "Splenic mediated erythrocyte cytotoxicity in malaria. Cell-mediated cytotoxicity in virulent rodent malaria has been demonstrated in vitro, whereby splenic cells effected specific lysis of 51Cr-labelled erythrocytes from parasitized animals. More than one cellular cytolytic effector system appeared to be operative in the mouse. One effector system involved splenic macrophages, from normal or immune animals, which were increasingly cytotoxic to target cells in the presence of antibody. A second effector system involved nylonpurified immune spleen cells which were significantly more cytotoxic than similary prepared normal spleen cells in the presence of immune serum. Although antibody alone was not cytolytic, the data strengthen the concept that immune spleen cells and antibody can interact in a co-operative fashion to mediate cytotoxic reactions in malaria."}
{"id": "PMID:1095475", "title": "Experimental aspergillosis in rats infected via intraperitoneal and subcutaneous routes.", "content": "Normal adult rats infected via the subcutaneous (s.c.) route with viable spores of Aspergillus fumigatus develop serum antibodies, measured by passive haemagglutination and passive cutaneous anaphylaxis (PCA), to extracts of the fungus. No significant histological abnormality was detected but s.c. inoculation in cortisone-treated animals induced the formation of granulomas frequently found in lymph nodes including those of the mesentery and paratracheal group. These granulomas were associated with the presence of both spores and fungal hyphae. Intraperitoneal inoculation of A. fumigatus spores occasionally produced lesions containing hyphae in the liver, spleen and mesenteric lymph nodes. Lesions were more frequent and extensive in cortisone-treated rats where again they were most prominent in the mesenteric lymph nodes. None of the regimes produced fatal hyphal aspergillosis. Both normal and cortisone-treated rats developed serum agglutinins but not reagins following intraperitoneal injection of spores.", "contents": "Experimental aspergillosis in rats infected via intraperitoneal and subcutaneous routes. Normal adult rats infected via the subcutaneous (s.c.) route with viable spores of Aspergillus fumigatus develop serum antibodies, measured by passive haemagglutination and passive cutaneous anaphylaxis (PCA), to extracts of the fungus. No significant histological abnormality was detected but s.c. inoculation in cortisone-treated animals induced the formation of granulomas frequently found in lymph nodes including those of the mesentery and paratracheal group. These granulomas were associated with the presence of both spores and fungal hyphae. Intraperitoneal inoculation of A. fumigatus spores occasionally produced lesions containing hyphae in the liver, spleen and mesenteric lymph nodes. Lesions were more frequent and extensive in cortisone-treated rats where again they were most prominent in the mesenteric lymph nodes. None of the regimes produced fatal hyphal aspergillosis. Both normal and cortisone-treated rats developed serum agglutinins but not reagins following intraperitoneal injection of spores."}
{"id": "PMID:1095476", "title": "Incorporation of stainless steel, titanium and Vitallium in bone.", "content": "Operative trauma, implant instability and corrosion are recognized as aetiological factors in the production of the soft-tissue capsule often present round a metal implant in bone. In the present experimental study, the isolated effect of corrosion was evaluated under standardized experimental conditions when operative trauma was minimal and implant movement was negligible. The reaction of bone to stainless steel, titanium and Vitallium was studied by histological and biomechanical methods, and compared to that provoked by copper. No soft-tissue capsule was found round the modern materials, but copper gave rise to a marked tissue reaction. It is concluded that a traumatic operative techniques and implant immobility are more effective for stable incorporation of metallic implants in bone than the nature of the implant itself, provided that one of the modern, low-corrosive metals or alloys is used.", "contents": "Incorporation of stainless steel, titanium and Vitallium in bone. Operative trauma, implant instability and corrosion are recognized as aetiological factors in the production of the soft-tissue capsule often present round a metal implant in bone. In the present experimental study, the isolated effect of corrosion was evaluated under standardized experimental conditions when operative trauma was minimal and implant movement was negligible. The reaction of bone to stainless steel, titanium and Vitallium was studied by histological and biomechanical methods, and compared to that provoked by copper. No soft-tissue capsule was found round the modern materials, but copper gave rise to a marked tissue reaction. It is concluded that a traumatic operative techniques and implant immobility are more effective for stable incorporation of metallic implants in bone than the nature of the implant itself, provided that one of the modern, low-corrosive metals or alloys is used."}
{"id": "PMID:1095477", "title": "Osseous lesion simulating a bone tumour due to an unsuspected fragment of wood in the foot.", "content": "A case is reported in which an osteolytic lesion in the third metatarsal bone was proved at operation to be the result of an injury by a splinter of wood which had remained embedded in the tissues. A short review of the relevant literature is appended.", "contents": "Osseous lesion simulating a bone tumour due to an unsuspected fragment of wood in the foot. A case is reported in which an osteolytic lesion in the third metatarsal bone was proved at operation to be the result of an injury by a splinter of wood which had remained embedded in the tissues. A short review of the relevant literature is appended."}
{"id": "PMID:1095479", "title": "In vitro lymphocyte response to purified protein derivative, BCG and Mycobacterium leprae in a population not exposed to leprosy.", "content": "Lymphocytes from 14 BCG-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (PPD), PPD/BCG, whole BCG bacilli, and whole Mycobacterium leprae and also with Candida antigen and phytohemagglutinin. The response was measured by incorporation of (3)H-labeled thymidine. The response to PPD, PPD/BCG, and BCG was found to correlate with the result of skin testing with turberculin. The turberculin-positive group also responded more strongly to M. leprae, whereas the two groups did not differ significantly in their response to Candida antigen or phytohemagglutinin. These findings indicate a certain degree of cross-reactivity between BCG and M. leprae. The use of the lymphocyte transformation test to measure antigenic cross-reactivity is discussed.", "contents": "In vitro lymphocyte response to purified protein derivative, BCG and Mycobacterium leprae in a population not exposed to leprosy. Lymphocytes from 14 BCG-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (PPD), PPD/BCG, whole BCG bacilli, and whole Mycobacterium leprae and also with Candida antigen and phytohemagglutinin. The response was measured by incorporation of (3)H-labeled thymidine. The response to PPD, PPD/BCG, and BCG was found to correlate with the result of skin testing with turberculin. The turberculin-positive group also responded more strongly to M. leprae, whereas the two groups did not differ significantly in their response to Candida antigen or phytohemagglutinin. These findings indicate a certain degree of cross-reactivity between BCG and M. leprae. The use of the lymphocyte transformation test to measure antigenic cross-reactivity is discussed."}
{"id": "PMID:1095480", "title": "In vivo behavior of revertants from Stahylococcus aureus L-phase variants compared with the parent strain.", "content": "The in vivo behavior of revertants of Staphylococcus aureus L-phase variants were studied in mice. The Giorgio strain of S. aureus was induced into L-phase variants by methicillin. After 13 serial passages in methicillin agar and six passages in antibiotic-free agar, reversion occurred. Six revertants, all phage untypable, coagulase negative, and mannitol negative, were inoculated intravenously into Swiss albino mice in comparison with the parent strain. Inoculation of 10(8) colony-forming units resulted in 38% mortality at 28 days for the Giorgio group and 0 to 2% for the revertants. The geometric mean titer of staphylococci in the kidneys was 3.5 x 10(5) for the Giorgio group and 0.5 x 10(0) to 1.0 x 10(2) for the revertants. Serial colony counts of blood, lungs, spleen, liver, and kidneys in mice inoculated with 10(7) colony-forming units showed faster clearance and failure of the revertant to colonize the kidneys. It is concluded that the in vivo behavior of the L-phase revertants was vastly different from that of the parent Giorgio strain.", "contents": "In vivo behavior of revertants from Stahylococcus aureus L-phase variants compared with the parent strain. The in vivo behavior of revertants of Staphylococcus aureus L-phase variants were studied in mice. The Giorgio strain of S. aureus was induced into L-phase variants by methicillin. After 13 serial passages in methicillin agar and six passages in antibiotic-free agar, reversion occurred. Six revertants, all phage untypable, coagulase negative, and mannitol negative, were inoculated intravenously into Swiss albino mice in comparison with the parent strain. Inoculation of 10(8) colony-forming units resulted in 38% mortality at 28 days for the Giorgio group and 0 to 2% for the revertants. The geometric mean titer of staphylococci in the kidneys was 3.5 x 10(5) for the Giorgio group and 0.5 x 10(0) to 1.0 x 10(2) for the revertants. Serial colony counts of blood, lungs, spleen, liver, and kidneys in mice inoculated with 10(7) colony-forming units showed faster clearance and failure of the revertant to colonize the kidneys. It is concluded that the in vivo behavior of the L-phase revertants was vastly different from that of the parent Giorgio strain."}
{"id": "PMID:1095481", "title": "Bacteremia in suckling rabbits after oral challenge with Vibrio parahaemolyticus.", "content": "Vibrio parahaemolyticus, a halophilic marine enteropathogen, produces diarrhea in man after ingestion of contaminated seafood. Only strains capable of producing beta-hemolysis (Kanagawa-positive strains) are enteropathogenic. Yet the majority of marine isolants are nonhemolytic and Kanagawa negative. Studies were initiated in suckling rabbits in an attempt to elucidate pathogenetic mechanisms. Fasting animals were infected orally with Kanagawa-positive and Kanagawa-negative strains of V. parahaemolyticus, V. cholerae, two enteropathogenic strains of Escherichia coli, Shigella flexneri, and strains of salmonellae. Seven hours postchallenge, cardiac blood, liver, and spleen cultures were obtained. V. parahaemolyticus strains failed to induce intestinal fluid accumulation during this study period. Bacteremia occurred in animals challenged with Kanagawa-positive V. parahaemolyticus, S. flexneri, and salmonella strains only. Animal passage increased the ability of V. parahaemolyticus to cause bacteremia. Liver and spleen cultures were positive in approximately 50% of animals challenged with the Kanagawa-positive strains of V. parphaemolyticus. The ability of this organism to penetrate the intestinal epithelium of suckling rabbits may represent a specific property that plays a role in pathogenesis.", "contents": "Bacteremia in suckling rabbits after oral challenge with Vibrio parahaemolyticus. Vibrio parahaemolyticus, a halophilic marine enteropathogen, produces diarrhea in man after ingestion of contaminated seafood. Only strains capable of producing beta-hemolysis (Kanagawa-positive strains) are enteropathogenic. Yet the majority of marine isolants are nonhemolytic and Kanagawa negative. Studies were initiated in suckling rabbits in an attempt to elucidate pathogenetic mechanisms. Fasting animals were infected orally with Kanagawa-positive and Kanagawa-negative strains of V. parahaemolyticus, V. cholerae, two enteropathogenic strains of Escherichia coli, Shigella flexneri, and strains of salmonellae. Seven hours postchallenge, cardiac blood, liver, and spleen cultures were obtained. V. parahaemolyticus strains failed to induce intestinal fluid accumulation during this study period. Bacteremia occurred in animals challenged with Kanagawa-positive V. parahaemolyticus, S. flexneri, and salmonella strains only. Animal passage increased the ability of V. parahaemolyticus to cause bacteremia. Liver and spleen cultures were positive in approximately 50% of animals challenged with the Kanagawa-positive strains of V. parphaemolyticus. The ability of this organism to penetrate the intestinal epithelium of suckling rabbits may represent a specific property that plays a role in pathogenesis."}
{"id": "PMID:1095482", "title": "Lymphocyte transformation in syphilis: an in vitro correlate of immune suppression in vivo?", "content": "Suppression of cellular immunity during primary and secondary infection may explain, in part, the unusual clinical evolution of syphilis. We have previously shown that lymphocytes from normal subjects undergo blastic transformation when exposed in vitro to Treponema refringens. This response was suppressed in patients with syphilis. the suppression being unrelated to serum factors. In the present paper we studied lymphocyte response in vitro to T. refringens, T. reiter, and T. pallidum as well as to monilia and trychophytins. The response to these antigens was suppressed in patients with syphilis although the response to phytohemagglutinin. pokeweed mitogen, and streptolysin was normal. These data support the hypothesis that human infection with T. pallidum is followed by a complex interaction between cellular and humoral immunity, the former being suppressed in primary and secondary stages.", "contents": "Lymphocyte transformation in syphilis: an in vitro correlate of immune suppression in vivo? Suppression of cellular immunity during primary and secondary infection may explain, in part, the unusual clinical evolution of syphilis. We have previously shown that lymphocytes from normal subjects undergo blastic transformation when exposed in vitro to Treponema refringens. This response was suppressed in patients with syphilis. the suppression being unrelated to serum factors. In the present paper we studied lymphocyte response in vitro to T. refringens, T. reiter, and T. pallidum as well as to monilia and trychophytins. The response to these antigens was suppressed in patients with syphilis although the response to phytohemagglutinin. pokeweed mitogen, and streptolysin was normal. These data support the hypothesis that human infection with T. pallidum is followed by a complex interaction between cellular and humoral immunity, the former being suppressed in primary and secondary stages."}
{"id": "PMID:1095483", "title": "Role of immunoglobulins in protection against Shigella-induced keratoconjunctives.", "content": "Various immunoglobulin preparations were tested for their ability to protect guinea pig eyes from infection by a virulent strain of Shigella flexneri. Secretory immunoglobulin A was effective in delaying or preventing keratoconjunctivitis in eight guinea pigs when it was used to precoat the organism, and was also placed in the eye with the inoculum. Neither immunoglobulin G nor immunoglobulin M gave any protection when used in the same way. Protection by secretory immunoglobulin A appeared to be related to the antishigella antibody content of the immunoglobulin since a low-titered preparation gave less protection than a higher-titered one.", "contents": "Role of immunoglobulins in protection against Shigella-induced keratoconjunctives. Various immunoglobulin preparations were tested for their ability to protect guinea pig eyes from infection by a virulent strain of Shigella flexneri. Secretory immunoglobulin A was effective in delaying or preventing keratoconjunctivitis in eight guinea pigs when it was used to precoat the organism, and was also placed in the eye with the inoculum. Neither immunoglobulin G nor immunoglobulin M gave any protection when used in the same way. Protection by secretory immunoglobulin A appeared to be related to the antishigella antibody content of the immunoglobulin since a low-titered preparation gave less protection than a higher-titered one."}
{"id": "PMID:1095484", "title": "Altered phospholipid metabolism in Escherichia coli accompanying killing by disrupted granulocytes.", "content": "The effect of bactericidal concentrations of disrupted rabbit granulocytes and of partially purified granulocyte fractions on phospholipid metabolism by Escherichia coli has been investigated. Previous studies in this laboratory have shown that, during and after killing of E. coli by granulocytes, bacterial macromolecular synthesis continues. Similarly, despite almost complete loss of viability within 15 min, incorporation of [1-(14)C]palmitate, [2-(14)C]glycerol, and [1-(14)C]acetate into E. coli phospholipids, in the presence of granulocyte preparations, remains the same as in control E. coli populations for at least 1 h. Incorporation of [1-(14)C]oleate into E. coli phospholipids is actually stimulated during the first 60 min of incubation in the presence of granulocyte preparations (more than twofold at 30 min and 40% at 60 min). With all labeled lipid precursors, bactericidal granulocyte preparations cause a relative increase in the labeling of E. coli cardiolipin, with a corresponding drop in labeled phosphatidyl-glycerol. Labeled lyso-compounds accumulate in the presence of granulocyte preparations when [1-(14)C]palmitate, but not when [1-(14)C]oleate is the labeled precursor. Since oleate occurs mainly in the 2-acyl position of E. coli phospholipids, whereas at least 50% of palmitate occurs in the 1 position, it appears that a phospholipase A(2) acts on the E. coli phospholipids. These various effects are also seen when E. coli are exposed to highly purified granulocyte preparations that possess potent bactericidal and phospholipase A(2) activities. We speculate that this phospholipase A(2) in the granulocyte preparations stimulates oleate but not palmitate incorporation by initiating increased turnover of the fatty acid in the 2-acyl position of E. coli phospholipids, causing formation of 1-acyl lyso-compounds likely to be preferentially reacylated with unsaturated fatty acids.", "contents": "Altered phospholipid metabolism in Escherichia coli accompanying killing by disrupted granulocytes. The effect of bactericidal concentrations of disrupted rabbit granulocytes and of partially purified granulocyte fractions on phospholipid metabolism by Escherichia coli has been investigated. Previous studies in this laboratory have shown that, during and after killing of E. coli by granulocytes, bacterial macromolecular synthesis continues. Similarly, despite almost complete loss of viability within 15 min, incorporation of [1-(14)C]palmitate, [2-(14)C]glycerol, and [1-(14)C]acetate into E. coli phospholipids, in the presence of granulocyte preparations, remains the same as in control E. coli populations for at least 1 h. Incorporation of [1-(14)C]oleate into E. coli phospholipids is actually stimulated during the first 60 min of incubation in the presence of granulocyte preparations (more than twofold at 30 min and 40% at 60 min). With all labeled lipid precursors, bactericidal granulocyte preparations cause a relative increase in the labeling of E. coli cardiolipin, with a corresponding drop in labeled phosphatidyl-glycerol. Labeled lyso-compounds accumulate in the presence of granulocyte preparations when [1-(14)C]palmitate, but not when [1-(14)C]oleate is the labeled precursor. Since oleate occurs mainly in the 2-acyl position of E. coli phospholipids, whereas at least 50% of palmitate occurs in the 1 position, it appears that a phospholipase A(2) acts on the E. coli phospholipids. These various effects are also seen when E. coli are exposed to highly purified granulocyte preparations that possess potent bactericidal and phospholipase A(2) activities. We speculate that this phospholipase A(2) in the granulocyte preparations stimulates oleate but not palmitate incorporation by initiating increased turnover of the fatty acid in the 2-acyl position of E. coli phospholipids, causing formation of 1-acyl lyso-compounds likely to be preferentially reacylated with unsaturated fatty acids."}
{"id": "PMID:1095485", "title": "Isolation and purification of Treponema pallidum from syphilitic lesions in rabbits.", "content": "Treponema pallidum were extracted from testicular syphilomas of corticosteroid-treated rabbits and purified by differential centrifugation. The steroid therapy allowed a longer holding time for infected rabbits, which produced greater treponeme yields, averaging 1.58 x 10(10) treponemes per rabbit. The treatment, which also diminished cellular infiltration and increased the extracellular mucoid material in lesions, produced much cleaner suspensions than preparations from nontreated animals. Most of the treponemes in the purified suspensions were still motile, and none carried demonstrable host immunoglobulin. The preparations were free of recognizable host tissue debris and they contained, on the average, 1.9 x 10(-7) mug of protein per treponeme.", "contents": "Isolation and purification of Treponema pallidum from syphilitic lesions in rabbits. Treponema pallidum were extracted from testicular syphilomas of corticosteroid-treated rabbits and purified by differential centrifugation. The steroid therapy allowed a longer holding time for infected rabbits, which produced greater treponeme yields, averaging 1.58 x 10(10) treponemes per rabbit. The treatment, which also diminished cellular infiltration and increased the extracellular mucoid material in lesions, produced much cleaner suspensions than preparations from nontreated animals. Most of the treponemes in the purified suspensions were still motile, and none carried demonstrable host immunoglobulin. The preparations were free of recognizable host tissue debris and they contained, on the average, 1.9 x 10(-7) mug of protein per treponeme."}
{"id": "PMID:1095486", "title": "Simplified assay for concanavalin A-dependent bacterial agglutination by using cell surface mutants.", "content": "Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A.", "contents": "Simplified assay for concanavalin A-dependent bacterial agglutination by using cell surface mutants. Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A."}
{"id": "PMID:1095487", "title": "Yersinia pestis: correlation of ultrastructures and immunological status.", "content": "Ultrastructural identification and localization of the fraction 1 \"envelope\" antigen in the plague bacillus Yersinia pestis were the primary objectives of this brief study. The antigenicity of extra-cellular material between the bacilli in undisturbed cultured colonies and that of the pathogen per se were measured and correlated by means of the semi quantitative complement fixation method after incubation for 72 h at 37 C. When the amount of extracellular substance in wild-type T1 (virulent) bacteria was compared by electron microscopy with that in avirulent strains of Y. pestis, with and without passage through guinea pigs, we found that the material of interest was greatly attenuated or even absent in colonies that had not been passed through animals, whereas passage markedly augmented production of the material. We also explored the requirement for larger quantities of Ca(2+) and Mg(2+) in the culture media and discovered that without these ions production of the extracellular material was limited. These observations support the hypothesis that this extracellular substance between cultured Y. pestis bacilli of various strains represents the source of the fraction 1 envelope antigen.", "contents": "Yersinia pestis: correlation of ultrastructures and immunological status. Ultrastructural identification and localization of the fraction 1 \"envelope\" antigen in the plague bacillus Yersinia pestis were the primary objectives of this brief study. The antigenicity of extra-cellular material between the bacilli in undisturbed cultured colonies and that of the pathogen per se were measured and correlated by means of the semi quantitative complement fixation method after incubation for 72 h at 37 C. When the amount of extracellular substance in wild-type T1 (virulent) bacteria was compared by electron microscopy with that in avirulent strains of Y. pestis, with and without passage through guinea pigs, we found that the material of interest was greatly attenuated or even absent in colonies that had not been passed through animals, whereas passage markedly augmented production of the material. We also explored the requirement for larger quantities of Ca(2+) and Mg(2+) in the culture media and discovered that without these ions production of the extracellular material was limited. These observations support the hypothesis that this extracellular substance between cultured Y. pestis bacilli of various strains represents the source of the fraction 1 envelope antigen."}
{"id": "PMID:1095488", "title": "In vitro N-nitrosodimethylamine formation by some bacteria.", "content": "It was found that human pathogenic bacteria and some species of intestinal bacteria in rats have the ability to catalyze the formation of N-nitrosodimethylamine from dimethylamine and nitrate in vitro.", "contents": "In vitro N-nitrosodimethylamine formation by some bacteria. It was found that human pathogenic bacteria and some species of intestinal bacteria in rats have the ability to catalyze the formation of N-nitrosodimethylamine from dimethylamine and nitrate in vitro."}
{"id": "PMID:1095489", "title": "Genetic instability of M protein and serum opacity factor of group A streptocci: evidence suggesting extrachromosomal control.", "content": "The M antigen, a primary determinant of virulence in group A streptococci that is expressed biologically as resistance to phagocytosis, is known to undergo a variety of phenotypic changes both in vivo and in vitro. These changes are nonrandom and can occur at a high frequency. Using the previously described relationship between the serum opacity reaction (associated with certain strains) and the presence of the M antigen, the phenotypic instability of the M antigen was analyzed. The results support the conclusion that M protein synthesis and the serum opacity reaction are directly or indirectly controlled by the same gene or by genes which are linked and can segregate as a unit. Moreover, growth conditions and the curing agents rifampin and ethidium bromide had a discernible influence on the segregation of clones unable to exhibit serum opacity factor and to resist phagocytosis by human leukocytes. Serial transfer of stationary-phase cultures of four strains of group A streptococci significantly increased the number of colonies negative for the serum opacity reaction and the M antigen. For two of four strains both ethidium bromide and rifampin also increased the segregation of colonies with this phenotype. In light of these experiments and the necessary controls, the possible influence of plasmids or bacteriophage in regulating M protein synthesis is discussed.", "contents": "Genetic instability of M protein and serum opacity factor of group A streptocci: evidence suggesting extrachromosomal control. The M antigen, a primary determinant of virulence in group A streptococci that is expressed biologically as resistance to phagocytosis, is known to undergo a variety of phenotypic changes both in vivo and in vitro. These changes are nonrandom and can occur at a high frequency. Using the previously described relationship between the serum opacity reaction (associated with certain strains) and the presence of the M antigen, the phenotypic instability of the M antigen was analyzed. The results support the conclusion that M protein synthesis and the serum opacity reaction are directly or indirectly controlled by the same gene or by genes which are linked and can segregate as a unit. Moreover, growth conditions and the curing agents rifampin and ethidium bromide had a discernible influence on the segregation of clones unable to exhibit serum opacity factor and to resist phagocytosis by human leukocytes. Serial transfer of stationary-phase cultures of four strains of group A streptococci significantly increased the number of colonies negative for the serum opacity reaction and the M antigen. For two of four strains both ethidium bromide and rifampin also increased the segregation of colonies with this phenotype. In light of these experiments and the necessary controls, the possible influence of plasmids or bacteriophage in regulating M protein synthesis is discussed."}
{"id": "PMID:1095490", "title": "Factors affecting filamentation in Candida albicans: changes in respiratory activity of Candida albicans during filamentation.", "content": "Glucose metabolism and respiration of Candida albicans were compared under conditions which permitted either maximal filamentous or maximal yeast growth. Changes in metabolism were monitored by comparing the quantities of ethanol produced, CO2 evolved, and oxygen consumed. Filamenting cultures produced more ethanol and less CO2 than yeasts, with oxygen consumption in the former concomitantly slower than that of the latter. Studies involving cofactors and inhibitors associated with electron transport imply that a transfer of electrons away from flavoprotein is required for maintenance of yeast morphology. Conditions consistent with a buildup of reduced flavoprotein, however, favored filament formation. These changes were expressed metabolically as a shift from an aerobic to a fermentative metabolism. The results presented are consistent with hypotheses correlating filament production with changes in carbohydrate metabolism and an interruption of electron transfer within the cell.", "contents": "Factors affecting filamentation in Candida albicans: changes in respiratory activity of Candida albicans during filamentation. Glucose metabolism and respiration of Candida albicans were compared under conditions which permitted either maximal filamentous or maximal yeast growth. Changes in metabolism were monitored by comparing the quantities of ethanol produced, CO2 evolved, and oxygen consumed. Filamenting cultures produced more ethanol and less CO2 than yeasts, with oxygen consumption in the former concomitantly slower than that of the latter. Studies involving cofactors and inhibitors associated with electron transport imply that a transfer of electrons away from flavoprotein is required for maintenance of yeast morphology. Conditions consistent with a buildup of reduced flavoprotein, however, favored filament formation. These changes were expressed metabolically as a shift from an aerobic to a fermentative metabolism. The results presented are consistent with hypotheses correlating filament production with changes in carbohydrate metabolism and an interruption of electron transfer within the cell."}
{"id": "PMID:1095491", "title": "Cellular streptolysin S-related hemolysins of group A Streptococcus C203S.", "content": "Group A streptococci strain C203S, grown in heart infusion broth with 0.3% maltose, produce two cellular hemolysins related to extracellular streptolysin S (SLS). Enzymatic lysis of the streptococci by group C streptococcal phage-associated lysin results in release of low titer, labile hemolysin, which can be stabilized by ribonucleic acid (RNA)-core (RNA preparation from yeast). This labile hemolysin can be detected only after the higher titer cellular streptolysin O is removed by erythrocyte membranes or inactivated by N-ethylmaleimide. The other cellular SLS-related hemolysin is released in a latent state (potential hemolysin) which can be activated to high-titer hemolysin by sonication with RNA-core. The titer of such activated hemolysin depends upon the intensity of sonic energy, duration of sonication, and amount of RNA-core. RNA obtained from the streptococci is far less effective than RNA-core. When the cocci are disrupted by sonication or grinding, potential hemolysin and/or activated form may be released, depending upon the conditions employed. The potential hemolysin material is large and heterogeneous; activation appears to involve, in part, disaggregation or fragmentation. Labile hemolysin, potential hemolysin, and the activated form of potential hemolysin can all be converted to hemolysin having the same hemolytic and physical properties as RNA-core SLS, suggesting that all have the same hemolytic moiety. The presence of glucose in heart infusion broth prevents formation of both potential hemolysin and RNA-core SLS by log-phase cells, whereas addition of glucose to a culture in heart infusion broth with 0.3% maltose stops accumulation of potential hemolysin but does not affect continuation of RNA-core SLS release. These results suggest that potential hemolysin is a cellular precursor to RNA-core SLS.", "contents": "Cellular streptolysin S-related hemolysins of group A Streptococcus C203S. Group A streptococci strain C203S, grown in heart infusion broth with 0.3% maltose, produce two cellular hemolysins related to extracellular streptolysin S (SLS). Enzymatic lysis of the streptococci by group C streptococcal phage-associated lysin results in release of low titer, labile hemolysin, which can be stabilized by ribonucleic acid (RNA)-core (RNA preparation from yeast). This labile hemolysin can be detected only after the higher titer cellular streptolysin O is removed by erythrocyte membranes or inactivated by N-ethylmaleimide. The other cellular SLS-related hemolysin is released in a latent state (potential hemolysin) which can be activated to high-titer hemolysin by sonication with RNA-core. The titer of such activated hemolysin depends upon the intensity of sonic energy, duration of sonication, and amount of RNA-core. RNA obtained from the streptococci is far less effective than RNA-core. When the cocci are disrupted by sonication or grinding, potential hemolysin and/or activated form may be released, depending upon the conditions employed. The potential hemolysin material is large and heterogeneous; activation appears to involve, in part, disaggregation or fragmentation. Labile hemolysin, potential hemolysin, and the activated form of potential hemolysin can all be converted to hemolysin having the same hemolytic and physical properties as RNA-core SLS, suggesting that all have the same hemolytic moiety. The presence of glucose in heart infusion broth prevents formation of both potential hemolysin and RNA-core SLS by log-phase cells, whereas addition of glucose to a culture in heart infusion broth with 0.3% maltose stops accumulation of potential hemolysin but does not affect continuation of RNA-core SLS release. These results suggest that potential hemolysin is a cellular precursor to RNA-core SLS."}
{"id": "PMID:1095492", "title": "Use of the K88 antigen for in vivo bacterial competition with porcine strains of enteropathogenic Escherichia coli.", "content": "Infant mice were used to measure the amount of fluid accumulation (enterosorption) in the intestinal tract after oral inoculation of a porcine strain of enteropathogenic Escherichia coli (K88-+, Ent-+). Significant reduction in the amount of fluid found in the intestinal tract was observed if the mice were first inoculated with a K88-possessing, non-enterotoxigenic strain of E. coli. The protection provided is thought to be due to specific competition for attachment sites on cells of the small intestine.", "contents": "Use of the K88 antigen for in vivo bacterial competition with porcine strains of enteropathogenic Escherichia coli. Infant mice were used to measure the amount of fluid accumulation (enterosorption) in the intestinal tract after oral inoculation of a porcine strain of enteropathogenic Escherichia coli (K88-+, Ent-+). Significant reduction in the amount of fluid found in the intestinal tract was observed if the mice were first inoculated with a K88-possessing, non-enterotoxigenic strain of E. coli. The protection provided is thought to be due to specific competition for attachment sites on cells of the small intestine."}
{"id": "PMID:1095493", "title": "Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication.", "content": "L cells (mouse fibroblasts) supported the multiplication of the obligately intracellular parasitic bacterium Chlamydia psittaci (strain 6BC) when incubated in fresh growth medium (medium 199 + 5% fetal calf serum). When incubated in the medium supernatant from a 24-h-old culture of uninfected L cells (24-h used medium), uninfected cells did not divide and infected cells did not provide an adequate environment for the multiplication of C. psittaci, which persisted in a noninfectious latent state within the host cells. The failure of both L cells and chlamydiae to divide resulted from an overall reduction in the rate of protein synthesis by both host and parasite brought about by an insufficiency of the essential amino acid isoleucine in 24-h used medium. The concentration of isoleucine required to activate minimal growth of C. psittaci also minimally stimulated uninfected L cells to divide. The addition of cycloheximide to 24-h used medium also activated the latent chlamydial infection because it stimulated the incorporation of host protein-derived isoleucine into chlamydial protein. The results suggest that the chlamydial parasite and the L-cell host compete for the isoleucine in the soluble pool of the host cell and that the parasite is capable of sequestering isoleucine for its own biosynthetic needs only when the concentration of isoleucine in the host pool rises above the level required to maintain the hose in the stationary state. Extrapolation of the results obtained with the L cell-C. psittaci model system to natural latent chlamydial infections is discussed.", "contents": "Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. L cells (mouse fibroblasts) supported the multiplication of the obligately intracellular parasitic bacterium Chlamydia psittaci (strain 6BC) when incubated in fresh growth medium (medium 199 + 5% fetal calf serum). When incubated in the medium supernatant from a 24-h-old culture of uninfected L cells (24-h used medium), uninfected cells did not divide and infected cells did not provide an adequate environment for the multiplication of C. psittaci, which persisted in a noninfectious latent state within the host cells. The failure of both L cells and chlamydiae to divide resulted from an overall reduction in the rate of protein synthesis by both host and parasite brought about by an insufficiency of the essential amino acid isoleucine in 24-h used medium. The concentration of isoleucine required to activate minimal growth of C. psittaci also minimally stimulated uninfected L cells to divide. The addition of cycloheximide to 24-h used medium also activated the latent chlamydial infection because it stimulated the incorporation of host protein-derived isoleucine into chlamydial protein. The results suggest that the chlamydial parasite and the L-cell host compete for the isoleucine in the soluble pool of the host cell and that the parasite is capable of sequestering isoleucine for its own biosynthetic needs only when the concentration of isoleucine in the host pool rises above the level required to maintain the hose in the stationary state. Extrapolation of the results obtained with the L cell-C. psittaci model system to natural latent chlamydial infections is discussed."}
{"id": "PMID:1095494", "title": "Prolonging morbidity in rabid dogs by intrathecal injection of attenuated rabies vaccine.", "content": "Dogs vaccinated intrathecally with attenuated rabies vaccine developed antibodies that were detected in the cerebrospinal fluid, blood, and brain; dogs similarly vaccinated but with an inactivated vaccine developed no antibodies in the brain. When the attenuated vaccine was administered to rabid dogs, a prolongation of the morbidity period was noted and, in some dogs, recovery from the disease. Rhesus monkeys died when administered any of the available attenuated vaccines intrathecally, and further studies with that species could not be undertaken.", "contents": "Prolonging morbidity in rabid dogs by intrathecal injection of attenuated rabies vaccine. Dogs vaccinated intrathecally with attenuated rabies vaccine developed antibodies that were detected in the cerebrospinal fluid, blood, and brain; dogs similarly vaccinated but with an inactivated vaccine developed no antibodies in the brain. When the attenuated vaccine was administered to rabid dogs, a prolongation of the morbidity period was noted and, in some dogs, recovery from the disease. Rhesus monkeys died when administered any of the available attenuated vaccines intrathecally, and further studies with that species could not be undertaken."}
{"id": "PMID:1095495", "title": "Quantitation of human peripheral blood T and B lymphocytes.", "content": "Centrifugation of heparinized peripheral blood on Ficoll-Hypaque under defined conditions is a most common method of lymphocyte purification in clinical studies. A loss of 25-30% of lymphocytes may occur during this procedure due to incomplete recovery of lymphocytes from the plasma layer after the gradient centrifugation and/or to poor recovery during the washing process. The loss is not selective since T and B cells are lost in the same proportions as determied by several different methods for T and B cell identification. Quantitation of T and B lymphocytes in terms of total number/mm-3 of peripheral blood is necessary in order to determine deficiencies in either of these two cell populations. The percentage values are inadequate in clinical evaluations. Thus, patients with chronic lymphocytic leukemia show elevated absolute number of T cells absolute numbers of T and B cells as well as percentages ought to be reported in all clinical studies.", "contents": "Quantitation of human peripheral blood T and B lymphocytes. Centrifugation of heparinized peripheral blood on Ficoll-Hypaque under defined conditions is a most common method of lymphocyte purification in clinical studies. A loss of 25-30% of lymphocytes may occur during this procedure due to incomplete recovery of lymphocytes from the plasma layer after the gradient centrifugation and/or to poor recovery during the washing process. The loss is not selective since T and B cells are lost in the same proportions as determied by several different methods for T and B cell identification. Quantitation of T and B lymphocytes in terms of total number/mm-3 of peripheral blood is necessary in order to determine deficiencies in either of these two cell populations. The percentage values are inadequate in clinical evaluations. Thus, patients with chronic lymphocytic leukemia show elevated absolute number of T cells absolute numbers of T and B cells as well as percentages ought to be reported in all clinical studies."}
{"id": "PMID:1095496", "title": "In vitro lymphocyte transformation in the development of experimental allergic thyroiditis.", "content": "In vitro lymphocyte transformation to homologous thyroid antigen has been studied in cultures of lymph node cells obtained from 97 guinea pigs at weekly intervals after a single injection of homologous thyroid extract in complete Freund's adjuvant, using a method for measurement of 3-H-thymidine incorporation. Lymph node cell cultures from animals 1 week after sensitization showed a significant increase in thymidine incorporation after the addition of antigen. Two weeks after sensitization the stimulation was maximal, and then a lower but significant response continued during the next 5 weeks. There was no correlation between lymphocyte transformation and the level of circulating antibodies throughout the post-sensitization period examined. The early lymphocyte transformation contrasted with the later development of macrophage migration inhibitory factor activity.", "contents": "In vitro lymphocyte transformation in the development of experimental allergic thyroiditis. In vitro lymphocyte transformation to homologous thyroid antigen has been studied in cultures of lymph node cells obtained from 97 guinea pigs at weekly intervals after a single injection of homologous thyroid extract in complete Freund's adjuvant, using a method for measurement of 3-H-thymidine incorporation. Lymph node cell cultures from animals 1 week after sensitization showed a significant increase in thymidine incorporation after the addition of antigen. Two weeks after sensitization the stimulation was maximal, and then a lower but significant response continued during the next 5 weeks. There was no correlation between lymphocyte transformation and the level of circulating antibodies throughout the post-sensitization period examined. The early lymphocyte transformation contrasted with the later development of macrophage migration inhibitory factor activity."}
{"id": "PMID:1095499", "title": "Human, rat and mouse liver-mediated mutagenicity of vinyl chloride in S. typhimurium strains.", "content": "Exposure of S. typhimurium strains TA 1530, TA 1535 and G-46 to vinyl chloride increased the number of His+ revertants/plate 16, 12 or 5 times over the spontaneous mutation rate. After 6 h of exposure to vinyl chloride, the mutagenic response for TA 1530 strain was enhanced 7-, 4- or 5-fold when fortified postmitochondrial liver fractions from humans, rats or mice were added. The enzyme-mediated vinyl chloride mutagenicity was dependent on an NADPH generating system and the enzyme activity was localized in a liver microsomal fraction; 9,000 times g liver supernatant was three times more active than microsomes, while liver cytosol or alcohol dehydrogenase did not affect the mutagenicity. Phenobarbitone pretreatment of rats and mice increased the mutagenic response by up to 15-40 percent as compared to untreated controls. The relative mutagenic activities of VCM, taking the value from mouse liver as 100, for TA 1530 strain mediated by 9,000 times g tissue fractions were: rat liver, 80; mouse and rat kidney, 20 and 16; mouse and rat lung, less than 7; human liver (from four biopsy specimens) 170, 64, 70 and 46. Chloroacetaldehyde and chloroacetic acid, a urinary metabolite of VCM, showed toxic effects, while chloroethanol was weakly mutagenic for TA 1530 strain.", "contents": "Human, rat and mouse liver-mediated mutagenicity of vinyl chloride in S. typhimurium strains. Exposure of S. typhimurium strains TA 1530, TA 1535 and G-46 to vinyl chloride increased the number of His+ revertants/plate 16, 12 or 5 times over the spontaneous mutation rate. After 6 h of exposure to vinyl chloride, the mutagenic response for TA 1530 strain was enhanced 7-, 4- or 5-fold when fortified postmitochondrial liver fractions from humans, rats or mice were added. The enzyme-mediated vinyl chloride mutagenicity was dependent on an NADPH generating system and the enzyme activity was localized in a liver microsomal fraction; 9,000 times g liver supernatant was three times more active than microsomes, while liver cytosol or alcohol dehydrogenase did not affect the mutagenicity. Phenobarbitone pretreatment of rats and mice increased the mutagenic response by up to 15-40 percent as compared to untreated controls. The relative mutagenic activities of VCM, taking the value from mouse liver as 100, for TA 1530 strain mediated by 9,000 times g tissue fractions were: rat liver, 80; mouse and rat kidney, 20 and 16; mouse and rat lung, less than 7; human liver (from four biopsy specimens) 170, 64, 70 and 46. Chloroacetaldehyde and chloroacetic acid, a urinary metabolite of VCM, showed toxic effects, while chloroethanol was weakly mutagenic for TA 1530 strain."}
{"id": "PMID:1095500", "title": "[Aminoglycoside antibiotics from clinical viewpoint].", "content": "Indications, side effects, dosage and several pharmacokinetic properties of gentamicin, tobramycin, sisomicin, kanamycin, amikacin, and streptomycin are delt with in the first part. Hereafter, some aspects are briefly mentioned concerning combinations of antibiotics with aminoglycosides, as well as indications for the evaluation and analysis of the aminoglycoside concentration in the serum.", "contents": "[Aminoglycoside antibiotics from clinical viewpoint]. Indications, side effects, dosage and several pharmacokinetic properties of gentamicin, tobramycin, sisomicin, kanamycin, amikacin, and streptomycin are delt with in the first part. Hereafter, some aspects are briefly mentioned concerning combinations of antibiotics with aminoglycosides, as well as indications for the evaluation and analysis of the aminoglycoside concentration in the serum."}
{"id": "PMID:1095501", "title": "[Aminopenicillins from microbiological viewpoint].", "content": "In this report it was tried to emphasize several microbiological aspects of the amino-penicillin. Hereby it turns out that the activity differences of all the substances are very similar on microbiological part. A break of the cross-resistance does not happen with all the amino-penicillins. With regard to the appearance of resistant strains, it can be told that the conditions still are advantageous on non-hospitalized patients in the totality. In the clinic a high part of resistant strains are found on different grounds.", "contents": "[Aminopenicillins from microbiological viewpoint]. In this report it was tried to emphasize several microbiological aspects of the amino-penicillin. Hereby it turns out that the activity differences of all the substances are very similar on microbiological part. A break of the cross-resistance does not happen with all the amino-penicillins. With regard to the appearance of resistant strains, it can be told that the conditions still are advantageous on non-hospitalized patients in the totality. In the clinic a high part of resistant strains are found on different grounds."}
{"id": "PMID:1095502", "title": "Aminopenicillins - clinical pharmacology and use in disease states.", "content": "Ampicillin introduced in 1961 has been administered in the treatment of diverse infections by both oral and parenteral means. Oral infections of the upper airways such as otitis media, bronchitis, and pneumonia have responded with high success rates since the microorganisms involved have remained sensitive to ampicillin. Similarly, out-patient urinary tract infections caused by Escherichia coli, Proteus mirabilis, and enterococci are cured. Typhoid fever may yet be treated with ampicillin, but shigellosis has become refractory with the development of resistant strains. Ampicillin has assumed a prominent role in the treatment of gonorrhoea. Parenteral ampicillin is still a mainstay of the treatment of Hemophilus meningitis, but the recent appearance of ampicillin resistant strains may become a serious problem. A number of derivatives and analogues of ampicillin have been developed. Among the compounds, hetacillin, metampicillin and pivampicillin which hydrolyze in the body to yield ampicillin, only pivampicillin appears to offer advantage over the parent compound. Blood levels are twice those of a comparable dose of ampicillin. However, more comparisons with ampicillin in clinical situations are needed. The other analogues of ampicillin are epicillin, cyclacillin and amoxicillin. Epicillin has no superiority to ampicillin, and the cyclacillin data do not show clear superiority over ampicillin in spite of initially high blood levels, since the compound is less active and so rapidly cleared from the body. Amoxicillin, on the other hand, has been shown to have it vitro activity equal to ampicillin and to produce higher blood levels for a longer period of time. Clinical studies have substantiated efficacy in treatment of otitis media, pharyngitis, bronchitis, pneumonitis, and urinary tract infections at doses half those of ampicillin. It has been effective in gonorrhoea and typhoid, but not in shigellosis. It would seem that to date only pivampicillin and amoxicillin, particularly the later, should be considered as replacements of ampicillin in oral therapy.", "contents": "Aminopenicillins - clinical pharmacology and use in disease states. Ampicillin introduced in 1961 has been administered in the treatment of diverse infections by both oral and parenteral means. Oral infections of the upper airways such as otitis media, bronchitis, and pneumonia have responded with high success rates since the microorganisms involved have remained sensitive to ampicillin. Similarly, out-patient urinary tract infections caused by Escherichia coli, Proteus mirabilis, and enterococci are cured. Typhoid fever may yet be treated with ampicillin, but shigellosis has become refractory with the development of resistant strains. Ampicillin has assumed a prominent role in the treatment of gonorrhoea. Parenteral ampicillin is still a mainstay of the treatment of Hemophilus meningitis, but the recent appearance of ampicillin resistant strains may become a serious problem. A number of derivatives and analogues of ampicillin have been developed. Among the compounds, hetacillin, metampicillin and pivampicillin which hydrolyze in the body to yield ampicillin, only pivampicillin appears to offer advantage over the parent compound. Blood levels are twice those of a comparable dose of ampicillin. However, more comparisons with ampicillin in clinical situations are needed. The other analogues of ampicillin are epicillin, cyclacillin and amoxicillin. Epicillin has no superiority to ampicillin, and the cyclacillin data do not show clear superiority over ampicillin in spite of initially high blood levels, since the compound is less active and so rapidly cleared from the body. Amoxicillin, on the other hand, has been shown to have it vitro activity equal to ampicillin and to produce higher blood levels for a longer period of time. Clinical studies have substantiated efficacy in treatment of otitis media, pharyngitis, bronchitis, pneumonitis, and urinary tract infections at doses half those of ampicillin. It has been effective in gonorrhoea and typhoid, but not in shigellosis. It would seem that to date only pivampicillin and amoxicillin, particularly the later, should be considered as replacements of ampicillin in oral therapy."}
{"id": "PMID:1095503", "title": "[Cephalosporin antibiotics from microbiologic viewpoint. A comparison of antibacterial and pharmacokinetic properties].", "content": "With the necessity not only of differentiating between effectiveness and non-effectiveness, as in the earlier phases of chemotherapy, but also of detecting graduated differences in effect quantitatively, animal experiment and clinical trial have been shown to be no longer adequate. In a correlating comparison of antibacterial activity with the drug level in vivo as the two decisive basic elements of chemotherapeutic effect, new and interesting possibilities arise for the evaluation of antibiotics. In a comparative evaluation of nine different cephalosporin antibiotics, not only the objective antibacterial and pharmacokinetic properties are taken into consideration, but also the dosage recommendations of the manufacturers as subjective factors. The result is that the cephalosporins in the treatment of gram-positive infections (except enterococci) are almost equivalent. In the gram-negative field, there are marked differences which produce a different species-dependent therapeutic value for the various preparations. A fundamentally superior \"universal\" cephalosporin for all bacterial infections does not exist.", "contents": "[Cephalosporin antibiotics from microbiologic viewpoint. A comparison of antibacterial and pharmacokinetic properties]. With the necessity not only of differentiating between effectiveness and non-effectiveness, as in the earlier phases of chemotherapy, but also of detecting graduated differences in effect quantitatively, animal experiment and clinical trial have been shown to be no longer adequate. In a correlating comparison of antibacterial activity with the drug level in vivo as the two decisive basic elements of chemotherapeutic effect, new and interesting possibilities arise for the evaluation of antibiotics. In a comparative evaluation of nine different cephalosporin antibiotics, not only the objective antibacterial and pharmacokinetic properties are taken into consideration, but also the dosage recommendations of the manufacturers as subjective factors. The result is that the cephalosporins in the treatment of gram-positive infections (except enterococci) are almost equivalent. In the gram-negative field, there are marked differences which produce a different species-dependent therapeutic value for the various preparations. A fundamentally superior \"universal\" cephalosporin for all bacterial infections does not exist."}
{"id": "PMID:1095504", "title": "[Cephalosporins from the clinical viewpoint].", "content": "Cephalosporines are discussed as viewed by the clinician. For the clinician it is difficult to choose among the offer of manifold preparations the most promising. Antibacterial activity, pharmacologial properties and possible side-effects are shown and discussed in all Cephalosporines to make the clinic's choice easier.", "contents": "[Cephalosporins from the clinical viewpoint]. Cephalosporines are discussed as viewed by the clinician. For the clinician it is difficult to choose among the offer of manifold preparations the most promising. Antibacterial activity, pharmacologial properties and possible side-effects are shown and discussed in all Cephalosporines to make the clinic's choice easier."}
{"id": "PMID:1095505", "title": "Peptide synthesis with carbodiimide.", "content": "An isotope dilution assay for racemization during acid couplings is described and applied to carbodiimide-mediated synthesis. Using the coupling of t-BOC-L-phenylalamine with glycine derivatives as a model, racemization in solution falls in the 0.01 to  0.1% range and it is a function of reagent concentrations. A typical coupling using the solid phase method shows 0.03% racemate.", "contents": "Peptide synthesis with carbodiimide. An isotope dilution assay for racemization during acid couplings is described and applied to carbodiimide-mediated synthesis. Using the coupling of t-BOC-L-phenylalamine with glycine derivatives as a model, racemization in solution falls in the 0.01 to  0.1% range and it is a function of reagent concentrations. A typical coupling using the solid phase method shows 0.03% racemate."}
{"id": "PMID:1095506", "title": "[Mutagenicity testing of irradiated laboratory animal feed in the 'host-mediated' assay with Salmonella typhimurium G 46].", "content": "Food irradiated with 10 MeV-electrons (dose: 4-5 Mrad) was tested for mutagenic effects, using the host-mediated assay. Irradiated 'Altomin' (a pelleted laboratory diet) was fed to mice for 9 to 44 days. After the 30th day of feeding, the animals additionally received irradiated glucose-solution instead of drinking water. Results did not indicate any mutagenic activity of the irradiated diet on the histidine-auxotroph strain of Salmonella typhimurium used in the assay.", "contents": "[Mutagenicity testing of irradiated laboratory animal feed in the 'host-mediated' assay with Salmonella typhimurium G 46]. Food irradiated with 10 MeV-electrons (dose: 4-5 Mrad) was tested for mutagenic effects, using the host-mediated assay. Irradiated 'Altomin' (a pelleted laboratory diet) was fed to mice for 9 to 44 days. After the 30th day of feeding, the animals additionally received irradiated glucose-solution instead of drinking water. Results did not indicate any mutagenic activity of the irradiated diet on the histidine-auxotroph strain of Salmonella typhimurium used in the assay."}
{"id": "PMID:1095507", "title": "The lung-colony assay: extension to the Lewis lung tumour and the B16 melanoma--radiosensitivity of B16 melanoma cells.", "content": "Experiments are described which demonstrate that a lung-colony assay can be used to study the viability of unknown cell populations from the B16 Melanoma or the Lewis Lung Tumour. It is shown that the number of lung colonies formed can be increased by the addition of plastic microspheres to the injected cell suspension or by pre-irradiating the lungs of the recipient mice. The colony technique has been used to isolate melanotic and amelanotic cell-lines from the B16 Melanoma which were found to have different growth-rates. In vitro radiation survival curves for B16 Melanoma cells have also been established, and these have parameters in the usual range for mammalian cells.", "contents": "The lung-colony assay: extension to the Lewis lung tumour and the B16 melanoma--radiosensitivity of B16 melanoma cells. Experiments are described which demonstrate that a lung-colony assay can be used to study the viability of unknown cell populations from the B16 Melanoma or the Lewis Lung Tumour. It is shown that the number of lung colonies formed can be increased by the addition of plastic microspheres to the injected cell suspension or by pre-irradiating the lungs of the recipient mice. The colony technique has been used to isolate melanotic and amelanotic cell-lines from the B16 Melanoma which were found to have different growth-rates. In vitro radiation survival curves for B16 Melanoma cells have also been established, and these have parameters in the usual range for mammalian cells."}
{"id": "PMID:1095510", "title": "Clinical trial of 3-methyl-5-(beta-N'-(N-m-chlorophenylpiperazino)ethyl)-pyrazole dihydrochloride (Mepiprazol) in the therapy of psychovegetative disorders.", "content": "In a clinical trial of 30 patients suffering from psychovegetative disorders and anxiety neuroses, Mepiprazol (test designation: EMD 16-923; supplier: E. Merck, Darmstadt, FRG) was proven in a treatment period of 14 days to be an effective, well-tolerated medication. Only two patients failed to respond during the course of treatment. Improvements were significant at the 1-percent level between the pretreatment condition and each control during the treatment period; even between the rating times there was a significant increment in treatment success. Mepiprazol had a particular effect on symptoms such as irritability, anxiety, inner unrest and tension. Mepiprazol also exhibited a positive effect in the treatment of psychosomatic complaints.", "contents": "Clinical trial of 3-methyl-5-(beta-N'-(N-m-chlorophenylpiperazino)ethyl)-pyrazole dihydrochloride (Mepiprazol) in the therapy of psychovegetative disorders. In a clinical trial of 30 patients suffering from psychovegetative disorders and anxiety neuroses, Mepiprazol (test designation: EMD 16-923; supplier: E. Merck, Darmstadt, FRG) was proven in a treatment period of 14 days to be an effective, well-tolerated medication. Only two patients failed to respond during the course of treatment. Improvements were significant at the 1-percent level between the pretreatment condition and each control during the treatment period; even between the rating times there was a significant increment in treatment success. Mepiprazol had a particular effect on symptoms such as irritability, anxiety, inner unrest and tension. Mepiprazol also exhibited a positive effect in the treatment of psychosomatic complaints."}
{"id": "PMID:1095511", "title": "[Double-blind study of Aprotininum (Trasylol) in the management of alcoholic delirium].", "content": "The results of uncontrolled studies, in which the period of the delirium tremens was reduced and the intensity was lowered significantly by aprotininum, could not be verified in our double-blind study. Continuing a preliminary double-blind study another group of 20 male patients was examined again to evaluate the effectiveness of aprotininum given in a modified experimental arrangement. Not only at the beginning of the treatment but also every 6 h an infusion of 200,000 KIE Trasylol was given during the period of the acute alcohol withdrawal syndromes. Time as well as intensity of symptoms (quantitatively measured by needed chlormethiazol) did not differ significantly between the aprotininum and the placebo group.", "contents": "[Double-blind study of Aprotininum (Trasylol) in the management of alcoholic delirium]. The results of uncontrolled studies, in which the period of the delirium tremens was reduced and the intensity was lowered significantly by aprotininum, could not be verified in our double-blind study. Continuing a preliminary double-blind study another group of 20 male patients was examined again to evaluate the effectiveness of aprotininum given in a modified experimental arrangement. Not only at the beginning of the treatment but also every 6 h an infusion of 200,000 KIE Trasylol was given during the period of the acute alcohol withdrawal syndromes. Time as well as intensity of symptoms (quantitatively measured by needed chlormethiazol) did not differ significantly between the aprotininum and the placebo group."}
{"id": "PMID:1095512", "title": "Psychotherapeutic profile of a new psychoactive drug trazodone (AF-1161).", "content": "In a systematic clinical study, including four uncontrolled clinical trials with psychiatric patients belonging to four different diagnostic groups, trazodone, the first triazolopyridine compound subjected to clinical investigation, was found to be a therapeutically effective psychoactive drug. Since the most consistent therapeutic action of trazodone was seen to be in the depressive syndrome, i.e., Guilt Feelings, Motor Retardation and Depressive Mood, it was suggested that trazodone has a therapeutic effect on depressive symptoms whether they occur in depression, neurosis, organic brain syndrome or schizophrenia.", "contents": "Psychotherapeutic profile of a new psychoactive drug trazodone (AF-1161). In a systematic clinical study, including four uncontrolled clinical trials with psychiatric patients belonging to four different diagnostic groups, trazodone, the first triazolopyridine compound subjected to clinical investigation, was found to be a therapeutically effective psychoactive drug. Since the most consistent therapeutic action of trazodone was seen to be in the depressive syndrome, i.e., Guilt Feelings, Motor Retardation and Depressive Mood, it was suggested that trazodone has a therapeutic effect on depressive symptoms whether they occur in depression, neurosis, organic brain syndrome or schizophrenia."}
{"id": "PMID:1095513", "title": "The outcome of dothiepin treatment in 1,900 depressed patients.", "content": "One thousand, nine hundred and sixty-five depressed patients took part in a multicentre study of dothiepin hydrochloride, which involved 392 doctors. The results indicated that a large percentage of patients improved as measured by all the assessments and no serious or adverse effects were encountered. A good correlation was seen between the global scale and the Rockliff depression scale; and also between the final mean Rockliff score and the number of patients stopping therapy due to recovery, and continuing treatment until recovery, at the final assessment.", "contents": "The outcome of dothiepin treatment in 1,900 depressed patients. One thousand, nine hundred and sixty-five depressed patients took part in a multicentre study of dothiepin hydrochloride, which involved 392 doctors. The results indicated that a large percentage of patients improved as measured by all the assessments and no serious or adverse effects were encountered. A good correlation was seen between the global scale and the Rockliff depression scale; and also between the final mean Rockliff score and the number of patients stopping therapy due to recovery, and continuing treatment until recovery, at the final assessment."}
{"id": "PMID:1095514", "title": "[Clinical trial of lofepramine versus imipramine].", "content": "Lofepramin is a new derivative of imipramine. Its effects and side effects were compared to those of imipramine in a double-blind trial. 49 hospitalized patients, suffering from endogenous depression, were treated for 20 days with 210 mg lofepramin daily and 52 other patients with 150 mg imipramine daily. The results of the repeated psychiatric examinations were documented using the AMP system. Both substances proved to have high antidepressant effects and showed no significant difference in the quality of their effects.", "contents": "[Clinical trial of lofepramine versus imipramine]. Lofepramin is a new derivative of imipramine. Its effects and side effects were compared to those of imipramine in a double-blind trial. 49 hospitalized patients, suffering from endogenous depression, were treated for 20 days with 210 mg lofepramin daily and 52 other patients with 150 mg imipramine daily. The results of the repeated psychiatric examinations were documented using the AMP system. Both substances proved to have high antidepressant effects and showed no significant difference in the quality of their effects."}
{"id": "PMID:1095522", "title": "The primary antibacterial defense mechanism of the bladder.", "content": "We have developed a model in rabbits for quantitatively determining the attachment of microbial organisms to the vesical mucosa. Our data suggest that the bladder has a primary antibacterial defense mechanism that serves to interrupt microbial attachment. This mechanism seems to be secretory, since after destruction with acid, the resistance to microbial binding returns in less than 24 hr.", "contents": "The primary antibacterial defense mechanism of the bladder. We have developed a model in rabbits for quantitatively determining the attachment of microbial organisms to the vesical mucosa. Our data suggest that the bladder has a primary antibacterial defense mechanism that serves to interrupt microbial attachment. This mechanism seems to be secretory, since after destruction with acid, the resistance to microbial binding returns in less than 24 hr."}
{"id": "PMID:1095528", "title": "Electrocardiographic changes following intravenous pyelography.", "content": "One hundred consecutive patients who underwent i.v. pyelography had ECG performed before, immediately following and 5 min after the injection. ECG changes appeared in 15 patients and were considered to be major in five of them. The incidence of ECG changes was significantly higher in patients with abnormal ECG prior to pyelography. The average age of the patients who developed changes was higher than that of patients in whom no ECG changes were observed. There was no significant difference in the incidence of ECG changes after standard and infusion pyelography.", "contents": "Electrocardiographic changes following intravenous pyelography. One hundred consecutive patients who underwent i.v. pyelography had ECG performed before, immediately following and 5 min after the injection. ECG changes appeared in 15 patients and were considered to be major in five of them. The incidence of ECG changes was significantly higher in patients with abnormal ECG prior to pyelography. The average age of the patients who developed changes was higher than that of patients in whom no ECG changes were observed. There was no significant difference in the incidence of ECG changes after standard and infusion pyelography."}
{"id": "PMID:1095530", "title": "Immunocytochemical localization of substance P in mammalian intestine.", "content": "In mammalian intestine immunoreactive Substance P is localized not only in the plexuses of Auerbach and Meissner, as could be anticipated, but also in a number of basally situated, often basigranular, endocrine cells which have been identified tentatively as enterochromaffin. The presence of a neurohormone in cells of this type confirms their close association with the nervous system, noted by Masson (1924), and suggests that their postulated origin from the nervous system (Danisch, 1924) may well be correct.", "contents": "Immunocytochemical localization of substance P in mammalian intestine. In mammalian intestine immunoreactive Substance P is localized not only in the plexuses of Auerbach and Meissner, as could be anticipated, but also in a number of basally situated, often basigranular, endocrine cells which have been identified tentatively as enterochromaffin. The presence of a neurohormone in cells of this type confirms their close association with the nervous system, noted by Masson (1924), and suggests that their postulated origin from the nervous system (Danisch, 1924) may well be correct."}
{"id": "PMID:1095531", "title": "Localization of substance P-like immunoreactivity in mouse gut.", "content": "Substance P immunoreactivity has been demonstrated in the mouse gut in two distinctly different locations; in the myenteric plexus of the proximal colon as well as in endocrine-like cells of the duodenal and colonic mucosa.", "contents": "Localization of substance P-like immunoreactivity in mouse gut. Substance P immunoreactivity has been demonstrated in the mouse gut in two distinctly different locations; in the myenteric plexus of the proximal colon as well as in endocrine-like cells of the duodenal and colonic mucosa."}
{"id": "PMID:1095532", "title": "Immunoperoxidase localisation of hepatitis B antigen (HB) in formalin-paraffin processed liver tissue.", "content": "The unlabelled antibody-enzyme (PAP) method was used to demonstrate HB in sections of routine formalin fixed paraffin embedded liver biopsies.", "contents": "Immunoperoxidase localisation of hepatitis B antigen (HB) in formalin-paraffin processed liver tissue. The unlabelled antibody-enzyme (PAP) method was used to demonstrate HB in sections of routine formalin fixed paraffin embedded liver biopsies."}
{"id": "PMID:1095533", "title": "The ultra-structural localization of enzyme activity in unfixed sections.", "content": "A technique is described for using conventional unfixed cryostat sections for localising enzyme activities in the electron microscope. The sections are incubated on the slide in the presence of a stabiliser, and then only fixed and embedded after the reaction is complete. Thin sections can then be cut of the reacted section, for electron microscopy. The present article shows that good ultra-structural morphology is retained during the freezing and cutting stages, and although some loss of detail occurs after the incubation, sub-cellular membranes are still intact, and membrane-associated reaction product can be clearly seen.", "contents": "The ultra-structural localization of enzyme activity in unfixed sections. A technique is described for using conventional unfixed cryostat sections for localising enzyme activities in the electron microscope. The sections are incubated on the slide in the presence of a stabiliser, and then only fixed and embedded after the reaction is complete. Thin sections can then be cut of the reacted section, for electron microscopy. The present article shows that good ultra-structural morphology is retained during the freezing and cutting stages, and although some loss of detail occurs after the incubation, sub-cellular membranes are still intact, and membrane-associated reaction product can be clearly seen."}
{"id": "PMID:1095534", "title": "Formaldehyde-ozone-induced fluorescence in isolated gastrin granules.", "content": "In several mammals the antral gastrin cells display characteristic formaldehyde-ozone-induced fluorescence (375/500-520 nm). Such fluorescence is also given by gastrinoma cells. Gastrin granules, isolated from gastrinomas and identified by immunofluorescence and electron microscopy, were found to show intense formaldehyde-ozone-induced fluorescence.", "contents": "Formaldehyde-ozone-induced fluorescence in isolated gastrin granules. In several mammals the antral gastrin cells display characteristic formaldehyde-ozone-induced fluorescence (375/500-520 nm). Such fluorescence is also given by gastrinoma cells. Gastrin granules, isolated from gastrinomas and identified by immunofluorescence and electron microscopy, were found to show intense formaldehyde-ozone-induced fluorescence."}
{"id": "PMID:1095542", "title": "Control of vascular volume in sheep umbilical circulation.", "content": "Vascular and extravascular volumes were measured using a single-injection, double-indicator technique, in a perfused umbilical circulation in which umbilical artery (Pfa) and umbilical vein (Pfv) pressures could be independently varied. At constant Pfv, when Pfa was raised from 25 to 35 mmHg, vascular volume increased 16.1  plus or minus 6 ml (mean plus or minus SD) from a control value of 113 plus or minus 38.3 ml. Extravascular volume increased 25.2 plus or minus 7.7 ml from 128 plus or minus 53.3 ml. During further increases in Pfa to 65 mmHg, vascular volume increased approximately 10 ml for each 10 mm Hg increment, but no change in extravascular volume occurred. At a constant Pfa when Pfv was raised to 15, 20, and 25 mmHg, vascular volume increased 12.2 plus or minus 3.6, 22.2 plus or minus 5.4, and 28 plus or minus 12.5 ml from the measurements at low Pfv. There was no increase in extravascular volume during elevation of Pfv. At the lower values of Pfa (25-35 mmHg) recruitment of previously unperfused channels and distension are seen. Over the higher ranges of Pfa change and during Pfv elevation there is distension of vascular bed.", "contents": "Control of vascular volume in sheep umbilical circulation. Vascular and extravascular volumes were measured using a single-injection, double-indicator technique, in a perfused umbilical circulation in which umbilical artery (Pfa) and umbilical vein (Pfv) pressures could be independently varied. At constant Pfv, when Pfa was raised from 25 to 35 mmHg, vascular volume increased 16.1  plus or minus 6 ml (mean plus or minus SD) from a control value of 113 plus or minus 38.3 ml. Extravascular volume increased 25.2 plus or minus 7.7 ml from 128 plus or minus 53.3 ml. During further increases in Pfa to 65 mmHg, vascular volume increased approximately 10 ml for each 10 mm Hg increment, but no change in extravascular volume occurred. At a constant Pfa when Pfv was raised to 15, 20, and 25 mmHg, vascular volume increased 12.2 plus or minus 3.6, 22.2 plus or minus 5.4, and 28 plus or minus 12.5 ml from the measurements at low Pfv. There was no increase in extravascular volume during elevation of Pfv. At the lower values of Pfa (25-35 mmHg) recruitment of previously unperfused channels and distension are seen. Over the higher ranges of Pfa change and during Pfv elevation there is distension of vascular bed."}
{"id": "PMID:1095543", "title": "Microbiological methods. Methodology for enteropathogenic Escherichia coli.", "content": "Pathogenic biotypes of Escherichia coli grow poorly at temperatures greatly different from that of the host. Percentages quantitatively recovered at 42.0, 44.0, 44.5, and 45.5 degrees C in lauryl tryptose broth were 100, 76, 76, and 42, respectively. Corresponding values for 175 strains of varied origin were 98, 89, 82, and 65%. Maximal growth temperature is dependent upon medium. Lauryl tryptose and elevated coliform broths were equivalent in the recovery of small inocula (100 cells/ml) at 41.5-44.5 degrees. MacConkey, enteric enrichment, and Gram-negative broths were inhibitory at corresponding values. Growth at elevated temperature in nutrient broth is enhanced by carbohydrate. Standard lactose enrichment media fail to recover slow lactose fermenters. An acidified glutamic acid medium was unsuitable for recovery of E. coli. The data suggest modification of standard temperatures for the recovery of pathogenic biotypes. Previously recommended analytical methods have been simplified and supplemented. The enhancement of motility in indole-nitrite broth at 35 degrees C is recommended. A 4-tube semiquantitative test is offered for tentative identification of somatic and capsular antigens. Inclusion of Alkalescens-Dispar strains is warranted by their pathogenic behavior. Examination in Shigella and Alkalescens-Dispar sera is required to cover the dysentery-like biotypes. Pathogenic potential cannot be inferred from serotype.", "contents": "Microbiological methods. Methodology for enteropathogenic Escherichia coli. Pathogenic biotypes of Escherichia coli grow poorly at temperatures greatly different from that of the host. Percentages quantitatively recovered at 42.0, 44.0, 44.5, and 45.5 degrees C in lauryl tryptose broth were 100, 76, 76, and 42, respectively. Corresponding values for 175 strains of varied origin were 98, 89, 82, and 65%. Maximal growth temperature is dependent upon medium. Lauryl tryptose and elevated coliform broths were equivalent in the recovery of small inocula (100 cells/ml) at 41.5-44.5 degrees. MacConkey, enteric enrichment, and Gram-negative broths were inhibitory at corresponding values. Growth at elevated temperature in nutrient broth is enhanced by carbohydrate. Standard lactose enrichment media fail to recover slow lactose fermenters. An acidified glutamic acid medium was unsuitable for recovery of E. coli. The data suggest modification of standard temperatures for the recovery of pathogenic biotypes. Previously recommended analytical methods have been simplified and supplemented. The enhancement of motility in indole-nitrite broth at 35 degrees C is recommended. A 4-tube semiquantitative test is offered for tentative identification of somatic and capsular antigens. Inclusion of Alkalescens-Dispar strains is warranted by their pathogenic behavior. Examination in Shigella and Alkalescens-Dispar sera is required to cover the dysentery-like biotypes. Pathogenic potential cannot be inferred from serotype."}
{"id": "PMID:1095544", "title": "Kanner's syndrome or early-onset psychosis: a taxonomic analysis of 142 cases.", "content": "Case history data on 142 psychotic children were obtained using the Rimland Diagnostic Check List. A particular variety of the method known as numerical taxonomy was used to classify the children into subgroups on the basis of responses on the Check List. A comparison was made between a classification of Kanner's syndrome vs. \"no Kanner's syndrome\" and a classification using random classes as starting points. The \"best\" classification of the data was one in which 2 classes were obtained, one containing all the Kanner's syndrome children plus other early-onset, nonrelating psychotic children and the other containing children with later onset and more varied symptoms. Those attributes which significantly differentiated between the classes both in the \"best\" classification and in the Kanner's syndrome classification were extracted. A distinction between Kanner children and other similar children was found only for 3 specific behaviors: obsessive desire for sameness, islets of special ability, and skillful manipulation of small objects.", "contents": "Kanner's syndrome or early-onset psychosis: a taxonomic analysis of 142 cases. Case history data on 142 psychotic children were obtained using the Rimland Diagnostic Check List. A particular variety of the method known as numerical taxonomy was used to classify the children into subgroups on the basis of responses on the Check List. A comparison was made between a classification of Kanner's syndrome vs. \"no Kanner's syndrome\" and a classification using random classes as starting points. The \"best\" classification of the data was one in which 2 classes were obtained, one containing all the Kanner's syndrome children plus other early-onset, nonrelating psychotic children and the other containing children with later onset and more varied symptoms. Those attributes which significantly differentiated between the classes both in the \"best\" classification and in the Kanner's syndrome classification were extracted. A distinction between Kanner children and other similar children was found only for 3 specific behaviors: obsessive desire for sameness, islets of special ability, and skillful manipulation of small objects."}
{"id": "PMID:1095545", "title": "Conservation of Salmonella typhimurium deoxyribonucleic acid by chromosomal insertion in a partially diploid Escherichia coli hybrid.", "content": "A partially diploid Escherichia coli hybrid recovered from mating with a Salmonella typhimurium donor was converted to an Hfr strain, designated WR2080, as a means to examine the manner in which the added Salmonella genetic material was conserved in it. The Salmonella argH-+, metB-+, and RHA-+ alleles contained as supernumerary genes in WR2080 were transferred together to E. coli recipients in interrupted mating experiments approximately 25 min after initial parental contact; transfer of the allelic E. coli genes by a  haploid Hfr of the same transfer orientation occurred between 23.5 min (argH-+) and 25 min (rha-+) after initial contact. Entry of the E. coli ilv-+ marker of WR2080 in these experiments occurred at 29.5 min, 1.5 min later than the entry time of this marker from the haploid E. coli Hfr. When  unselected inheritance of the recessive E. coli argH-minus and rha-minus alleles of WR2080 was examined among ilv-+ selected E. coli recipients in which unselected inheritance of the Salmonella donor genes was shown to be low (8%), inheritance of argH-minus was only 7%, whereas 51% inherited the neighboring rha-minus gene. In a comparative cross employing a haploid E. coli Hfr, in which rha inheritance was similar at 56%, argH inheritance was 41%. It was concluded that the Salmonella genes contained in WR2080 were conserved on a genetic segment about 1.5 min in length chromosomally inserted near the allelic E. coli genes, thus creating a duplication on that region within the hybrid chromosome.", "contents": "Conservation of Salmonella typhimurium deoxyribonucleic acid by chromosomal insertion in a partially diploid Escherichia coli hybrid. A partially diploid Escherichia coli hybrid recovered from mating with a Salmonella typhimurium donor was converted to an Hfr strain, designated WR2080, as a means to examine the manner in which the added Salmonella genetic material was conserved in it. The Salmonella argH-+, metB-+, and RHA-+ alleles contained as supernumerary genes in WR2080 were transferred together to E. coli recipients in interrupted mating experiments approximately 25 min after initial parental contact; transfer of the allelic E. coli genes by a  haploid Hfr of the same transfer orientation occurred between 23.5 min (argH-+) and 25 min (rha-+) after initial contact. Entry of the E. coli ilv-+ marker of WR2080 in these experiments occurred at 29.5 min, 1.5 min later than the entry time of this marker from the haploid E. coli Hfr. When  unselected inheritance of the recessive E. coli argH-minus and rha-minus alleles of WR2080 was examined among ilv-+ selected E. coli recipients in which unselected inheritance of the Salmonella donor genes was shown to be low (8%), inheritance of argH-minus was only 7%, whereas 51% inherited the neighboring rha-minus gene. In a comparative cross employing a haploid E. coli Hfr, in which rha inheritance was similar at 56%, argH inheritance was 41%. It was concluded that the Salmonella genes contained in WR2080 were conserved on a genetic segment about 1.5 min in length chromosomally inserted near the allelic E. coli genes, thus creating a duplication on that region within the hybrid chromosome."}
{"id": "PMID:1095546", "title": "Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A.", "content": "By using each of the available colicins, we have isolated a large number of colicin-resistant mutants. They included both receptor and tolerant mutants and each was screened for cross-resistance to all other colicins. On the basis of the cross-resistance of these mutants it was possible to place known colicins into two mutually exclusive groups, group A and group B. Mutants selected as resistant to colicins of group A may or may not be cross-resistant to other colicins of group A, BUT Are never resistant to colicins of group B. The reverse also applies. The mutants isolated as resistant to colicins of group A (A, E1, E2, E3, K, L, N, S4, and X) have been divided into 21 phenotypic classes on the basis of their colicin resistance patterns. These include most of the tolerant and receptor mutants previously isolated, some of which were previously shown to also have an increased sensitivity to certain antibiotics and detergents. Type strains from each of the phenotypic classes were therefore tested for sensitivity to a range of antibiotics, detergents, and surfactants that included all those previously used. With these new data, it has been possible to speculate informatively on the mode of action of the different colicins. We have confirmed the position of previously isolated mutations on the Escherichia coli K-12 genetic map, and located approximately the loci conferring colicin resistance in some of the newly isolated mutants.", "contents": "Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A. By using each of the available colicins, we have isolated a large number of colicin-resistant mutants. They included both receptor and tolerant mutants and each was screened for cross-resistance to all other colicins. On the basis of the cross-resistance of these mutants it was possible to place known colicins into two mutually exclusive groups, group A and group B. Mutants selected as resistant to colicins of group A may or may not be cross-resistant to other colicins of group A, BUT Are never resistant to colicins of group B. The reverse also applies. The mutants isolated as resistant to colicins of group A (A, E1, E2, E3, K, L, N, S4, and X) have been divided into 21 phenotypic classes on the basis of their colicin resistance patterns. These include most of the tolerant and receptor mutants previously isolated, some of which were previously shown to also have an increased sensitivity to certain antibiotics and detergents. Type strains from each of the phenotypic classes were therefore tested for sensitivity to a range of antibiotics, detergents, and surfactants that included all those previously used. With these new data, it has been possible to speculate informatively on the mode of action of the different colicins. We have confirmed the position of previously isolated mutations on the Escherichia coli K-12 genetic map, and located approximately the loci conferring colicin resistance in some of the newly isolated mutants."}
{"id": "PMID:1095547", "title": "dnaB gene of Escherichia coli K-12 affects superinfection inhibition between F' plasmids.", "content": "F' Escherichia coli K-12 strains bearing the chromosomal mutation dnaB43 offer significantly less resistance to the conjugational introduction of a second F' plasmid than do nonmutant strains. Both the entry exclusion and incompatibility components of superinfection inhibition are altered. This action of dnaB43 occurs regardless of the presence of a recA-minus mutation in matings in liquid cultures and on membrane filters and is not limited to a particular set of F' plasmids. These effects are co-transducible by phage P1 with the temperature sensitivity conferred by dnaB43. The effects also occur with a strain carrying dnaB107. In the double F' strains that arise, the two plasmids exist as units autonomous of one another and the chromosome.", "contents": "dnaB gene of Escherichia coli K-12 affects superinfection inhibition between F' plasmids. F' Escherichia coli K-12 strains bearing the chromosomal mutation dnaB43 offer significantly less resistance to the conjugational introduction of a second F' plasmid than do nonmutant strains. Both the entry exclusion and incompatibility components of superinfection inhibition are altered. This action of dnaB43 occurs regardless of the presence of a recA-minus mutation in matings in liquid cultures and on membrane filters and is not limited to a particular set of F' plasmids. These effects are co-transducible by phage P1 with the temperature sensitivity conferred by dnaB43. The effects also occur with a strain carrying dnaB107. In the double F' strains that arise, the two plasmids exist as units autonomous of one another and the chromosome."}
{"id": "PMID:1095548", "title": "Restoration of F' superinfection inhibition in a DnaB mutant of Escherichia coli upon construction of heterozygous DnaB merodiploids or P1 lysogens carrying a dnaB analogue.", "content": "F-prime derivatives of the Escherichia coli strain CR34 bearing the thermosensitivity mutation dnaB43 display low levels of plasmid-determined superinfection inhibition in conjugational crosses at 30 C. Salt-mediated phenotypic suppression of this temperature sensitivity fails to restore normal levels of inhibition, indicating its alteration is not a secondary effect of dnaB43 a-tion on growth or deoxyribonucleic acid syntheiss. Superinfection inhibition is fully restored in mutant cells made merodiploid for the dnaB region by introduction of the F' dnaB-+ plasmid F134-1. dnaB43-bearing strains lysogenized with P1 phage contribution dnaB-analogue protein show eight to nine times more superinfection inhibition than do the same cells carrying P1 prophage repressed dnaB-analogue protein production. Taken together, this evidence suggests a direct causal relationship between dnaB43 and the altered superinfection inhibition phenotype.", "contents": "Restoration of F' superinfection inhibition in a DnaB mutant of Escherichia coli upon construction of heterozygous DnaB merodiploids or P1 lysogens carrying a dnaB analogue. F-prime derivatives of the Escherichia coli strain CR34 bearing the thermosensitivity mutation dnaB43 display low levels of plasmid-determined superinfection inhibition in conjugational crosses at 30 C. Salt-mediated phenotypic suppression of this temperature sensitivity fails to restore normal levels of inhibition, indicating its alteration is not a secondary effect of dnaB43 a-tion on growth or deoxyribonucleic acid syntheiss. Superinfection inhibition is fully restored in mutant cells made merodiploid for the dnaB region by introduction of the F' dnaB-+ plasmid F134-1. dnaB43-bearing strains lysogenized with P1 phage contribution dnaB-analogue protein show eight to nine times more superinfection inhibition than do the same cells carrying P1 prophage repressed dnaB-analogue protein production. Taken together, this evidence suggests a direct causal relationship between dnaB43 and the altered superinfection inhibition phenotype."}
{"id": "PMID:1095549", "title": "Genetic and kinetic evidence for different types of postreplication repair in Escherichia coli B.", "content": "The changes in molecular weight of deoxyribonucleic acid (DNA) synthesized after ultraviolte irradiation of Escherichia coli WP28 uvrA, and strains additionally mutant at polA, exrA, recA, and exrA and polA loci, were examined by alkaline sucrose gradient centrifugation. In a repari=deficient uvrA recA strain, the frequency of breaks in newly synthesized DNA was equal to that for pyrimidine dimers in parental DNA. Measurements of the amounts and rates of postreplication repair of these breaks indicate that (i) repair is two to three times faster when DNA polymerase I is present, although (ii) almost all breaks are repaired regardless of DNA polymerase I activity. (iii) Increased ultraviolet doses lead to an increase in the proportion of breaks remaining unrepaired in uvrA recA, UVRA exrA, and uvrA exrA polA strains. The numbers of unrepaired breaks resemble the numbers expected if repair of one lesion is prevented by proximity of a second lesion.", "contents": "Genetic and kinetic evidence for different types of postreplication repair in Escherichia coli B. The changes in molecular weight of deoxyribonucleic acid (DNA) synthesized after ultraviolte irradiation of Escherichia coli WP28 uvrA, and strains additionally mutant at polA, exrA, recA, and exrA and polA loci, were examined by alkaline sucrose gradient centrifugation. In a repari=deficient uvrA recA strain, the frequency of breaks in newly synthesized DNA was equal to that for pyrimidine dimers in parental DNA. Measurements of the amounts and rates of postreplication repair of these breaks indicate that (i) repair is two to three times faster when DNA polymerase I is present, although (ii) almost all breaks are repaired regardless of DNA polymerase I activity. (iii) Increased ultraviolet doses lead to an increase in the proportion of breaks remaining unrepaired in uvrA recA, UVRA exrA, and uvrA exrA polA strains. The numbers of unrepaired breaks resemble the numbers expected if repair of one lesion is prevented by proximity of a second lesion."}
{"id": "PMID:1095550", "title": "Effect of uncouplers on \"downhill\" beta-galactoside transport in energy-depleted cells of Escherichia coli.", "content": "Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the \"downhill\" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhydrazone results in stimulation of ONPG transport. The same agents lower transport of non-energy-depleted cells towards the same levels that result from stimulation of the energy depleted cells. Of course, these agents prevent \"uphill\" accumulation against a concentration gradient under all conditions. Since uncouplers allow normal and energy-depleted cells to assume the same facilitated transport capability, these results lend support to the chemiosmotic hypothesis of Mitchell that comigration of charge is necessary for the transport of neutral galactosides. Our results imply that a potential favorable to transport is maintained by metabolism in non-energy-depleted cells, whereas an unfavorable potential is developed in the initial instant of time when energy-depleted cells are given ONPG.", "contents": "Effect of uncouplers on \"downhill\" beta-galactoside transport in energy-depleted cells of Escherichia coli. Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the \"downhill\" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhydrazone results in stimulation of ONPG transport. The same agents lower transport of non-energy-depleted cells towards the same levels that result from stimulation of the energy depleted cells. Of course, these agents prevent \"uphill\" accumulation against a concentration gradient under all conditions. Since uncouplers allow normal and energy-depleted cells to assume the same facilitated transport capability, these results lend support to the chemiosmotic hypothesis of Mitchell that comigration of charge is necessary for the transport of neutral galactosides. Our results imply that a potential favorable to transport is maintained by metabolism in non-energy-depleted cells, whereas an unfavorable potential is developed in the initial instant of time when energy-depleted cells are given ONPG."}
{"id": "PMID:1095551", "title": "Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1.", "content": "A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1.", "contents": "Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1. A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1."}
{"id": "PMID:1095552", "title": "Lambda bacteriophage gene produces and X-ray sensitivity of Escherichia coli: comparison of red-dependent and gam-dependent radioresistance.", "content": "When gene products of lambda bacteriophage are introduced into a cell by transient induction of a lysogen, increased resistance of the cells to X rays results. This phenomenon has been called phage-induced radioresistance. Genetic studies show at least two classes of induced radioresistance. The first type depends on the products of the lambda red genes and is observed in bacteria that are mutated in the recB gene. It is thought that the lambda red products compensate for the missing RecBC nuclease in the repair of X-ray damage. An optimal effect is obtained even when the lambda red products are supplied 1 h after irradiation. The lesions that are affected by the red-dependent process are probably not deoxyribonucleic acid strand breaks because the extent of deoxyribonucleic acid strand rejoining is not altered by the red products. The second type of phage-induced radioresistance requires the gam product of lambda and is observed in wild-type and polA strains. The lambda gam+ gene produce must be present immediately after irradiation to exert its full effect. In its presence, DNA breakdown is decreased, and a greater fraction of DNA is converted back to high molecular weight. Strains carrying lex, recA, or certain other combinations of mutations do not show any detectable phage-induced radioresistance.", "contents": "Lambda bacteriophage gene produces and X-ray sensitivity of Escherichia coli: comparison of red-dependent and gam-dependent radioresistance. When gene products of lambda bacteriophage are introduced into a cell by transient induction of a lysogen, increased resistance of the cells to X rays results. This phenomenon has been called phage-induced radioresistance. Genetic studies show at least two classes of induced radioresistance. The first type depends on the products of the lambda red genes and is observed in bacteria that are mutated in the recB gene. It is thought that the lambda red products compensate for the missing RecBC nuclease in the repair of X-ray damage. An optimal effect is obtained even when the lambda red products are supplied 1 h after irradiation. The lesions that are affected by the red-dependent process are probably not deoxyribonucleic acid strand breaks because the extent of deoxyribonucleic acid strand rejoining is not altered by the red products. The second type of phage-induced radioresistance requires the gam product of lambda and is observed in wild-type and polA strains. The lambda gam+ gene produce must be present immediately after irradiation to exert its full effect. In its presence, DNA breakdown is decreased, and a greater fraction of DNA is converted back to high molecular weight. Strains carrying lex, recA, or certain other combinations of mutations do not show any detectable phage-induced radioresistance."}
{"id": "PMID:1095553", "title": "Properties of the imidazolylacetolphosphate aminotransferase produced in a mutant demonstrating no apparent genetic involvement of the structural gene.", "content": "Genetic studies with strain hisBH22 of Salmonella typhimurium indicate it contains a deletion within the histidine operon involving part of the hisH gene and all of the hisB gene, but not extending into the adjacent hisC gene which is adjacent to hisB. However, the specific activity of the hisC product, imidazolylacetolphosphate aminotransferase (EC 2.6.1.9), in this strain is only 10 to 15% of that found in extracts from other mutants with a normal hisC gene. We have examined the rate of aminotransferase synthesis in this mutant and we find that the rate of synthesis of aminotransferase activity is low in mutant hisBH22, but the rate increases as the temperature of growth is lowered from 37 to 23 C. The low rate of enzyme accumulation is not due to holoenzyme instability at 37 C but instead is due to apoenzyme instability at this temperature. By transducing the hisBH22 marker into a pyridoxine auxotroph and derepressing the histidine operon under conditions where the intracellular concentration of pyridoxal phosphate would be expected to be low, we were able to demonstrate significant apoenzyme production only at the lower temperature. We suggest that the explanation for low aminotransferase specific activity at 37 C is due to the presence of reduced numbers of catalytically active units caused by normal production of an unstable mutant apoenzyme with only approximately 15% of the molecules being activated to holoenzyme. The holoenzyme from strain hisBH22 is stable during growth of this strain at 37 C.", "contents": "Properties of the imidazolylacetolphosphate aminotransferase produced in a mutant demonstrating no apparent genetic involvement of the structural gene. Genetic studies with strain hisBH22 of Salmonella typhimurium indicate it contains a deletion within the histidine operon involving part of the hisH gene and all of the hisB gene, but not extending into the adjacent hisC gene which is adjacent to hisB. However, the specific activity of the hisC product, imidazolylacetolphosphate aminotransferase (EC 2.6.1.9), in this strain is only 10 to 15% of that found in extracts from other mutants with a normal hisC gene. We have examined the rate of aminotransferase synthesis in this mutant and we find that the rate of synthesis of aminotransferase activity is low in mutant hisBH22, but the rate increases as the temperature of growth is lowered from 37 to 23 C. The low rate of enzyme accumulation is not due to holoenzyme instability at 37 C but instead is due to apoenzyme instability at this temperature. By transducing the hisBH22 marker into a pyridoxine auxotroph and derepressing the histidine operon under conditions where the intracellular concentration of pyridoxal phosphate would be expected to be low, we were able to demonstrate significant apoenzyme production only at the lower temperature. We suggest that the explanation for low aminotransferase specific activity at 37 C is due to the presence of reduced numbers of catalytically active units caused by normal production of an unstable mutant apoenzyme with only approximately 15% of the molecules being activated to holoenzyme. The holoenzyme from strain hisBH22 is stable during growth of this strain at 37 C."}
{"id": "PMID:1095554", "title": "Mode of action of alpha-dehydrobiotin, a biotin analogue.", "content": "Alpha-Dehydrobiotin, like biotin, represses coordinately the 7,8-diaminopelargonic acid aminotransferase and the dethiobiotin synthetase enzymes that are encoded on the l and r strands, respectively, of the bioA operon. The rate of synthesis for both enzymes is inhibited about 80% in the presence of alpha-dehydrobiotin. Homobiotin and alpha-methylbiotin are less effective than alpha-dehydrobiotin in repressing the synthesis of the two enzymes. The selective repression of transcription from l and by alpha-dehydrobiotin and homobiotin, previously reported in hybridization experiments, is not observed at the enzyme level. A combination of equal concentrations of biotin and alpha-dehydrobiotin which was reported to enhance selectively the level of messenger ribonucleic acid transcribed from the l strand does not increase the rate of synthesis of the aminotransferase enzyme. Instead, the enzymes encoded on both strands are essentially completely inhibited as with biotin alone. Strain differences have been ruled out to account for the different results obtained by the two methodologies. Our evidence would suggest that alpha-dehydrobiotin acts like biotin, presumably as the co-repressor, in the repression of the bioA operon. The low rates of enzyme synthesis observed in the presence of the biotin analogue is the result of incomplete repression due to a lower affinity of either the analogue for the repressor or of the co-repressor/repressor complex for the operator. While our evidence would support the concept of a two promoter/operator complex, both would have to respond equally to biotin and its analogues. The evidence, however, does not rule out other possible alternative models for the regulation of the biotin operon.", "contents": "Mode of action of alpha-dehydrobiotin, a biotin analogue. Alpha-Dehydrobiotin, like biotin, represses coordinately the 7,8-diaminopelargonic acid aminotransferase and the dethiobiotin synthetase enzymes that are encoded on the l and r strands, respectively, of the bioA operon. The rate of synthesis for both enzymes is inhibited about 80% in the presence of alpha-dehydrobiotin. Homobiotin and alpha-methylbiotin are less effective than alpha-dehydrobiotin in repressing the synthesis of the two enzymes. The selective repression of transcription from l and by alpha-dehydrobiotin and homobiotin, previously reported in hybridization experiments, is not observed at the enzyme level. A combination of equal concentrations of biotin and alpha-dehydrobiotin which was reported to enhance selectively the level of messenger ribonucleic acid transcribed from the l strand does not increase the rate of synthesis of the aminotransferase enzyme. Instead, the enzymes encoded on both strands are essentially completely inhibited as with biotin alone. Strain differences have been ruled out to account for the different results obtained by the two methodologies. Our evidence would suggest that alpha-dehydrobiotin acts like biotin, presumably as the co-repressor, in the repression of the bioA operon. The low rates of enzyme synthesis observed in the presence of the biotin analogue is the result of incomplete repression due to a lower affinity of either the analogue for the repressor or of the co-repressor/repressor complex for the operator. While our evidence would support the concept of a two promoter/operator complex, both would have to respond equally to biotin and its analogues. The evidence, however, does not rule out other possible alternative models for the regulation of the biotin operon."}
{"id": "PMID:1095555", "title": "Amplification of sex repressor function of one fi-+ R-factor during anaerobic growth of Escherichia coli.", "content": "It has been reported by Mitsuhashi (1965) that transfer of one R-factor was completely inhibited by anaerobic transfer conditions. In contrast, several workers have observed R-factor transfer, although at a reduced rate, in the animal intestines, a largely anaerobic environment. It is shown here that in vitro transfer of the R-factor R1 (F-type pilus, fi-+) in Escherichia coli K-12 is severely depressed, whereas transfer of R64 (I-type pilus, fi-minus) is slightly stimulated by anaerobiosis. Inhibition of R1 fertility is dependent on anaerobic conditions during pregrowth of the donor cells, whereas the oxygen tension during recipient pregrowth, transfer, and plating is of little importance. Anaerobic pregrowth has a less inhibitory effect on the fertility of R1drd19, a mutant of R1 having a defective sex repressor. The fi-+ property of R1 when introduced into F' or Hfr bacteria is amplified during anaerobic growth. These observations strongly indicate that the sex repressor is the mediator of the anaerobic fertility inhibition of the R-factor R1. This hypothesis was supported by studies of the formation of sex pili, the only gene product identified that is controlled by the sex repressor of R1. Using propagation of the F-type pilus-specific phage MS2 as a measure of the degree of sex piliation of a bacterial population, it is shown that in anaerobic cultures sex piliation due to R1 is strongly repressed, whereas piliation due to R1drd19 is repressed to a lesser extent. The possible survival value of the response of R1 towards oxygen tension is discussed.", "contents": "Amplification of sex repressor function of one fi-+ R-factor during anaerobic growth of Escherichia coli. It has been reported by Mitsuhashi (1965) that transfer of one R-factor was completely inhibited by anaerobic transfer conditions. In contrast, several workers have observed R-factor transfer, although at a reduced rate, in the animal intestines, a largely anaerobic environment. It is shown here that in vitro transfer of the R-factor R1 (F-type pilus, fi-+) in Escherichia coli K-12 is severely depressed, whereas transfer of R64 (I-type pilus, fi-minus) is slightly stimulated by anaerobiosis. Inhibition of R1 fertility is dependent on anaerobic conditions during pregrowth of the donor cells, whereas the oxygen tension during recipient pregrowth, transfer, and plating is of little importance. Anaerobic pregrowth has a less inhibitory effect on the fertility of R1drd19, a mutant of R1 having a defective sex repressor. The fi-+ property of R1 when introduced into F' or Hfr bacteria is amplified during anaerobic growth. These observations strongly indicate that the sex repressor is the mediator of the anaerobic fertility inhibition of the R-factor R1. This hypothesis was supported by studies of the formation of sex pili, the only gene product identified that is controlled by the sex repressor of R1. Using propagation of the F-type pilus-specific phage MS2 as a measure of the degree of sex piliation of a bacterial population, it is shown that in anaerobic cultures sex piliation due to R1 is strongly repressed, whereas piliation due to R1drd19 is repressed to a lesser extent. The possible survival value of the response of R1 towards oxygen tension is discussed."}
{"id": "PMID:1095556", "title": "Alterations of the rate of movement of deoxyribonucleic acid replication forks.", "content": "Antibiotics that inhibit ribonucleic acid (RNA) or protein synthesis are often used in studies of deoxyribonucleic acid (DNA) synthesis. The experiments presented here demonstrate that the rate of movement of DNA replication forks can be influenced by such antibotics. Addition of either chloramphenicol, which inhibits movement of ribosomes along messenger RNA, or streptolydigin, which inhibits movement of RNA polymerase, leads to a decrease in the rate of fork movement. Rifampin, which inhibits initiation of RNA synthesis, reverses the effects of chloramphenicol or streptolydigin. The slowed movement of DNA replication forks is discussed in terms of obstruction of fork movement by transcription complexes temporarily immobilized on the DNA template.", "contents": "Alterations of the rate of movement of deoxyribonucleic acid replication forks. Antibiotics that inhibit ribonucleic acid (RNA) or protein synthesis are often used in studies of deoxyribonucleic acid (DNA) synthesis. The experiments presented here demonstrate that the rate of movement of DNA replication forks can be influenced by such antibotics. Addition of either chloramphenicol, which inhibits movement of ribosomes along messenger RNA, or streptolydigin, which inhibits movement of RNA polymerase, leads to a decrease in the rate of fork movement. Rifampin, which inhibits initiation of RNA synthesis, reverses the effects of chloramphenicol or streptolydigin. The slowed movement of DNA replication forks is discussed in terms of obstruction of fork movement by transcription complexes temporarily immobilized on the DNA template."}
{"id": "PMID:1095557", "title": "Variation in expression of sex factor genes in the Proteus-Providencia group relative to Escherichia coli.", "content": "Several instances of anomalous expression of genes introduced from Escherichia coli K-12 into Proteus mirabilis have been described. It is shown here that control of sex pilus synthesis directed by the F-like R factor R1 and its depressed derivatives R1-16 (O-C) and R1-19 (i-minus) is also anomalous in P. mirabilis. Piliation in cells bearing the depressed plasmids is expressed at a lower level than in E. coli K-12, and repression is absent in R1-carrying cells. Preliminary results show a similar effect in Providencia. In Proteus morganii, a similarly reduced level of piliation in R1-16-+ or R1-19-+ cultures is observed, but an intermediate level of repression occurs in R1-+ cultures. Less extensive data suggest that expression of the sex factor genes of an R factor of the N incompatibility group differs far less between E. coli and P. mirabilis hosts. Possible bases for these effects are discussed.", "contents": "Variation in expression of sex factor genes in the Proteus-Providencia group relative to Escherichia coli. Several instances of anomalous expression of genes introduced from Escherichia coli K-12 into Proteus mirabilis have been described. It is shown here that control of sex pilus synthesis directed by the F-like R factor R1 and its depressed derivatives R1-16 (O-C) and R1-19 (i-minus) is also anomalous in P. mirabilis. Piliation in cells bearing the depressed plasmids is expressed at a lower level than in E. coli K-12, and repression is absent in R1-carrying cells. Preliminary results show a similar effect in Providencia. In Proteus morganii, a similarly reduced level of piliation in R1-16-+ or R1-19-+ cultures is observed, but an intermediate level of repression occurs in R1-+ cultures. Less extensive data suggest that expression of the sex factor genes of an R factor of the N incompatibility group differs far less between E. coli and P. mirabilis hosts. Possible bases for these effects are discussed."}
{"id": "PMID:1095558", "title": "RP1 properties and fertility inhibition among P, N, W, and X incompatibility group plasmids.", "content": "Incompatibility group P plasmids demonstrate strong entry exclusion properties. Stringent incompatibility is also observed in the absence of entry exclusion. These observations have been facilitated by the study of a nontransmissible plasmid, RP1-S2, derived from RP1 by transductional shortening. RP1-S2 retains carbenicillin and tetracycline resistances as well as loci that cause either the loss of P plasmids (incp) or a locus specifying susceptibility to curing (sinp) in the presence of a P plasmid. RP1-S2 can be mobilized by an incompatibility group W plasmid, R388, and also freely forms recombinants with R388. P, N, and W incompatibility group plasmids all encode information for the receptor of the cell wall-adsorbing phage PRD1. Based on the premise that the location of this receptor is analogous to entry exclusion factors for F-like plasmids and hence a regulated transfer region determinant, we tested fertility inhibition relationships among these plasmid groups. We detected both reciprocal and nonreciprocal fertility inhibition relationships for bacteria containing various combinations of W, N, and P group plasmids. The nonreciprocal nature of some combinations, we believe, reflects the identity of the point mutation reading to derepression of the plasmid in question. Reciprocal fertility inhibition, on the other hand, may reflect the reconstruction of a fertility inhibition system through complementation. An X incompatibility group plasmid, known to affect the fertility of an N group plasmid, was also shown to inhibit P plasmid fertility. These observations may indicate a possible evolutionary relationship(s) of plasmids unrelated by the criteria of incompatibility, pilus phage specificity, or plasmid host range.", "contents": "RP1 properties and fertility inhibition among P, N, W, and X incompatibility group plasmids. Incompatibility group P plasmids demonstrate strong entry exclusion properties. Stringent incompatibility is also observed in the absence of entry exclusion. These observations have been facilitated by the study of a nontransmissible plasmid, RP1-S2, derived from RP1 by transductional shortening. RP1-S2 retains carbenicillin and tetracycline resistances as well as loci that cause either the loss of P plasmids (incp) or a locus specifying susceptibility to curing (sinp) in the presence of a P plasmid. RP1-S2 can be mobilized by an incompatibility group W plasmid, R388, and also freely forms recombinants with R388. P, N, and W incompatibility group plasmids all encode information for the receptor of the cell wall-adsorbing phage PRD1. Based on the premise that the location of this receptor is analogous to entry exclusion factors for F-like plasmids and hence a regulated transfer region determinant, we tested fertility inhibition relationships among these plasmid groups. We detected both reciprocal and nonreciprocal fertility inhibition relationships for bacteria containing various combinations of W, N, and P group plasmids. The nonreciprocal nature of some combinations, we believe, reflects the identity of the point mutation reading to derepression of the plasmid in question. Reciprocal fertility inhibition, on the other hand, may reflect the reconstruction of a fertility inhibition system through complementation. An X incompatibility group plasmid, known to affect the fertility of an N group plasmid, was also shown to inhibit P plasmid fertility. These observations may indicate a possible evolutionary relationship(s) of plasmids unrelated by the criteria of incompatibility, pilus phage specificity, or plasmid host range."}
{"id": "PMID:1095559", "title": "Gentic properties of an R factor carrying resistance to aminolgycoside antibiotics.", "content": "R factor Rms 151 is an fi+ R factor and belongs to a incompatibility group FII. It carries the genes governing resistance to various aminoglycoside antibiotics, i.e., kanamycin (KM), lividomycin (LV), gentamicin C complex (GM), and 3',4'-dideoxykanamycin B (DKB), in addition to those governing to tetracycline (TC), chloramphenicol (CM), sulfanilamide (SA), and ampicillin (APC). Electron microscopy observation disclosed that the Rms151 deoxyribonucleic acid was a circular form with length of 31.2 mum. A probable circular genetic map of Rms151 was proposed by genetic and biochemical studies, the genes being in the order of -tet-tra-amp-aad-sul-aph-cml-, in which aad and aph confer resistance to KM.GM.DKB by adenylytransferase or resistance to KM.LV by phosphotransferase, respectively.", "contents": "Gentic properties of an R factor carrying resistance to aminolgycoside antibiotics. R factor Rms 151 is an fi+ R factor and belongs to a incompatibility group FII. It carries the genes governing resistance to various aminoglycoside antibiotics, i.e., kanamycin (KM), lividomycin (LV), gentamicin C complex (GM), and 3',4'-dideoxykanamycin B (DKB), in addition to those governing to tetracycline (TC), chloramphenicol (CM), sulfanilamide (SA), and ampicillin (APC). Electron microscopy observation disclosed that the Rms151 deoxyribonucleic acid was a circular form with length of 31.2 mum. A probable circular genetic map of Rms151 was proposed by genetic and biochemical studies, the genes being in the order of -tet-tra-amp-aad-sul-aph-cml-, in which aad and aph confer resistance to KM.GM.DKB by adenylytransferase or resistance to KM.LV by phosphotransferase, respectively."}
{"id": "PMID:1095560", "title": "Colicin tolerance and map location of conjugation-deficient mutants.", "content": "Con minus mutants, conjugation-defective mutants of Escherichia coli K-12, have been shown to be tolerant to colicins K and L. They map at approximately 14 min on the genetic map. The significance of the colicin tolerance of conjugation-defective mutants is discussed.", "contents": "Colicin tolerance and map location of conjugation-deficient mutants. Con minus mutants, conjugation-defective mutants of Escherichia coli K-12, have been shown to be tolerant to colicins K and L. They map at approximately 14 min on the genetic map. The significance of the colicin tolerance of conjugation-defective mutants is discussed."}
{"id": "PMID:1095561", "title": "Synchronization of cell division in Escherichia coli by amino acid starvation: strain specificity.", "content": "Synchronization of cell division by amino acid starvation can be induced in strains B and K-12 of Escherichia coli but not in strain B/r.", "contents": "Synchronization of cell division in Escherichia coli by amino acid starvation: strain specificity. Synchronization of cell division by amino acid starvation can be induced in strains B and K-12 of Escherichia coli but not in strain B/r."}
{"id": "PMID:1095562", "title": "Quantitation of the loss of the bacteriophage lambda receptor protein from the outer membrane of lipopolysaccharide-deficient strains of Escherichia coli.", "content": "The recpetor for the phage lambda, a protein component of the outer membrane, is present at decreased levels in strains of Escherichia coli that are deficient in lipopolysaccharide. Loss of the protein was quantitated both by an assay of the phage receptor function and by an assay of antiserum-blocking ability to detect inactive protein. The loss of protein was correlated with the loss of sugar residues and phosphage from the core region of the lipopolysaccharide. Implications for the importance of ionic interactions in the stabilization of the outer membrane are discussed.", "contents": "Quantitation of the loss of the bacteriophage lambda receptor protein from the outer membrane of lipopolysaccharide-deficient strains of Escherichia coli. The recpetor for the phage lambda, a protein component of the outer membrane, is present at decreased levels in strains of Escherichia coli that are deficient in lipopolysaccharide. Loss of the protein was quantitated both by an assay of the phage receptor function and by an assay of antiserum-blocking ability to detect inactive protein. The loss of protein was correlated with the loss of sugar residues and phosphage from the core region of the lipopolysaccharide. Implications for the importance of ionic interactions in the stabilization of the outer membrane are discussed."}
{"id": "PMID:1095563", "title": "Transition of the R factor NR1 and Proteus mirabilis: level of drug resistance of nontransitioned and transitioned cells.", "content": "When Proteus mirabilis harboring the R factor NR1 is cultured in Penassay broth containing 100 mug of chloramphenicol (CM) per ml, there is an amplification in the number of copies of the r-determinants per cell. Under these conditions, R factors harboring multiple tandem sequences of r-determinants are formed. Autonomous poly-f-determinants consisting of multiple copies of r-determinants are also formed. This phenomenon has been referred to as the \"transition\". Transitioned cells have considerably higher levels of resistance to CM and streptomycin (SM), but not to tetracycline (TC), than do nontransitioned cells and grow more rapidly in medium containing either CM or SM. There is essentially no difference in growth rates between transitioned and nontransitioned cells in drug-free medium. The higher level of resistance of transitioned cells to SM has made it possible to investigate the mechanism of the transition. Using replica plating, it has been possible to isolate spontaneously occurring transitioned cells from a nontransitioned population which appear to outgrow the nontransitioned cells during growth in medium containing 100 mug of CM per ml. If transiitoned cells are subsequently cultured in drug-free medium, the cells return gradually to the nontransitioned state, which has been referred to as the \"back-transition was monitored by examining the level of resisitance of the cells to SM. In both situations the cell populations were found to be heterogeneous, consisting of a mixture of nontransitioned and transitioned cells. Under the conditions of our experiments, the transition appeared to be due to the more rapid growth of a minor fraction of spontaneously occurring transitioned cells which outgrew the remainder of cells in the population. To obtain the transition, the drug resistance gene must reside on the r-determinants component of the R factor. The transition did not take place when the cells were cultured in medium containing high concentrations of TC. This indicates that the TC resistance genes reside on the resistance transfer factor component of the R factor, which is in agreement with physical studies on R factor deoxyribonucleic acid.", "contents": "Transition of the R factor NR1 and Proteus mirabilis: level of drug resistance of nontransitioned and transitioned cells. When Proteus mirabilis harboring the R factor NR1 is cultured in Penassay broth containing 100 mug of chloramphenicol (CM) per ml, there is an amplification in the number of copies of the r-determinants per cell. Under these conditions, R factors harboring multiple tandem sequences of r-determinants are formed. Autonomous poly-f-determinants consisting of multiple copies of r-determinants are also formed. This phenomenon has been referred to as the \"transition\". Transitioned cells have considerably higher levels of resistance to CM and streptomycin (SM), but not to tetracycline (TC), than do nontransitioned cells and grow more rapidly in medium containing either CM or SM. There is essentially no difference in growth rates between transitioned and nontransitioned cells in drug-free medium. The higher level of resistance of transitioned cells to SM has made it possible to investigate the mechanism of the transition. Using replica plating, it has been possible to isolate spontaneously occurring transitioned cells from a nontransitioned population which appear to outgrow the nontransitioned cells during growth in medium containing 100 mug of CM per ml. If transiitoned cells are subsequently cultured in drug-free medium, the cells return gradually to the nontransitioned state, which has been referred to as the \"back-transition was monitored by examining the level of resisitance of the cells to SM. In both situations the cell populations were found to be heterogeneous, consisting of a mixture of nontransitioned and transitioned cells. Under the conditions of our experiments, the transition appeared to be due to the more rapid growth of a minor fraction of spontaneously occurring transitioned cells which outgrew the remainder of cells in the population. To obtain the transition, the drug resistance gene must reside on the r-determinants component of the R factor. The transition did not take place when the cells were cultured in medium containing high concentrations of TC. This indicates that the TC resistance genes reside on the resistance transfer factor component of the R factor, which is in agreement with physical studies on R factor deoxyribonucleic acid."}
{"id": "PMID:1095564", "title": "Regulation of the Escherichia coli methylgalactoside transport system by gene mglD.", "content": "Constitutive activity of the methylgalactoside transport system of Escherichia coli K-12 is shown to result from mutation of a genetic locus distinct from the two previously described regulatory loci for this permease. Employing an autoradiographic procedure whereby constitutive and inducible cells can be differentiated, it is demonstrated that this locus, termed mglD, is 20% cotransducible with ptsF by bacteriophage P1. Selection for constitutive mutants among an inducible population yielded cells who mutations mapped in mglD. Cotransduction of mglD with mglB, minus C, and minus A, three genes required for activity of the methylgalactoside transport system, is 95, 88, and 81%, respectively. The results of recombination studies employing three and four factors indicate that the order of genes in this region is ptsF, mglD, B, C, A.", "contents": "Regulation of the Escherichia coli methylgalactoside transport system by gene mglD. Constitutive activity of the methylgalactoside transport system of Escherichia coli K-12 is shown to result from mutation of a genetic locus distinct from the two previously described regulatory loci for this permease. Employing an autoradiographic procedure whereby constitutive and inducible cells can be differentiated, it is demonstrated that this locus, termed mglD, is 20% cotransducible with ptsF by bacteriophage P1. Selection for constitutive mutants among an inducible population yielded cells who mutations mapped in mglD. Cotransduction of mglD with mglB, minus C, and minus A, three genes required for activity of the methylgalactoside transport system, is 95, 88, and 81%, respectively. The results of recombination studies employing three and four factors indicate that the order of genes in this region is ptsF, mglD, B, C, A."}
{"id": "PMID:1095565", "title": "Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K-12 and characterization of its transductants.", "content": "The envB1 mutation mediating a distorted cell morphology of Escherichia coliK-12 was cotransducible with strA, aroE, aspB, and argG. The mapping data is consistent with a gene location for envB around 62.5 min. In partial diploids envB1 was recessive to its wild-type allele. The original envB mutant contained a second mutation in a locus denoted sloB close to strA. The following gene order is suggested: sloB-strA-aroE-envB-aspB-argG. The sloB1 mutation caused a marked reduction in the growth rate of both envB and envB+ strains. Moreover, this mutation in the presence of envB1 appears to increase the ratio between deoxyribonucleic acid and protein in cells growing in rich medium. The phenotypic properties of envB1, sloB+ and envB+ transductants were characterized. Cells with envB1, sloB+ genotype were hypersensitive to several penicillins including the beta-lactam compound, amidino penicillin. Penicillin hypersensitivity could not be explained by increased outer membrane penetrability. The original envB mutant (envB1,SLOB1), as well as envB1, sloB1 or envB+, SLOB1 transductants were resistant to amidino penicillin. Resistance was explained by the slow growth rate mediated by the sloB1 mutation. The similarity between envB cells and wild-type cells treated with sublethal concentrations of amidino penicillin was emphasized.", "contents": "Genetic and physiological analysis of an envB spherelike mutant of Escherichia coli K-12 and characterization of its transductants. The envB1 mutation mediating a distorted cell morphology of Escherichia coliK-12 was cotransducible with strA, aroE, aspB, and argG. The mapping data is consistent with a gene location for envB around 62.5 min. In partial diploids envB1 was recessive to its wild-type allele. The original envB mutant contained a second mutation in a locus denoted sloB close to strA. The following gene order is suggested: sloB-strA-aroE-envB-aspB-argG. The sloB1 mutation caused a marked reduction in the growth rate of both envB and envB+ strains. Moreover, this mutation in the presence of envB1 appears to increase the ratio between deoxyribonucleic acid and protein in cells growing in rich medium. The phenotypic properties of envB1, sloB+ and envB+ transductants were characterized. Cells with envB1, sloB+ genotype were hypersensitive to several penicillins including the beta-lactam compound, amidino penicillin. Penicillin hypersensitivity could not be explained by increased outer membrane penetrability. The original envB mutant (envB1,SLOB1), as well as envB1, sloB1 or envB+, SLOB1 transductants were resistant to amidino penicillin. Resistance was explained by the slow growth rate mediated by the sloB1 mutation. The similarity between envB cells and wild-type cells treated with sublethal concentrations of amidino penicillin was emphasized."}
{"id": "PMID:1095566", "title": "Role of undecaprenyl phosphate in synthesis of polymers containing sialic acid in Escherichia coli.", "content": "Membrane-associated sialytransferase complexes from Escherichia coli K-235 catalyze the incorporation of N-acetylneuraminic acid (NeuNAc) from cytidine 5-monophospho-N-acetylneuraminic acid (CMP-NeuNAc) into polymeric products and a lipid fraction. Reconstitution of enzyme activity in lipid-depleted membrane complexes had an absolute dependence on a purified phospholipid which was characterized by high resolution mass spectrometry following dephosphorylation as undecaprenol. An identical mass spectrum was obtained on the phosphorylated lipid confirming that the active derivative was undecaprenyl phosphate. This C55-isoprenoid alcohol accounted for 95% of the polyisoprenol and contained 11 isoprene units, each one unsaturated. The remaining 5% was composed of the C60 homologue, dodecaprenol. Ficarprenyl phosphate, an isomer of undecaprenyl phosphate, also restored enzymatic activity although on a molar basis, it was less active than undecaprenyl phosphate. These results provide direct evidence that sialyl polymer synthesis has an obligatory requirement for undecaprenyl phosphate, a membrane-bound lipid coenzyme which functions as an intermediate carrier of glycosyl residues in the biosynthesis of a variety of microbial cell surface polysaccharides. These data extend further the general class of polysaccharides whose synthesis involves undecaprenyl phosphate and are in accord with the hypothesis that this lipid acts as an intermediate carrier of sialyl residues in sialyl polymer synthesis according to the following reaction: CMP-NeuNAc plus P-undecaprenol in equilibrium NeuNAc-P-undecaprenol plus CMP Although the sialylated lipid remains to be characterized, evidence in support of this conclusion was obtained by kinetic analysis of N-acetylneuraminic acid transfer into the lipid soluble fraction and sialyl polymers. These studies showed a rapid incorporation of N-acetylneuraminic acid into the lipid-soluble fraction prior to maximal sialyl polymer formation, an observation consistent with a possible precursortion, an observation consistent with a possible precursor-product relationship. Confirmation that the radioactivity in the lipid-soluble fraction was lipid-linked N-acetylneuraminic acid was provided by the demonstration of the formation of a transitory sialyl-lipid with chromatographic properties expected of sialyl-undecaprenyl phosphate. Three additional lines of investigation implicated a functional role for undecaprenyl phosphate in sialyl polymer synthesis...", "contents": "Role of undecaprenyl phosphate in synthesis of polymers containing sialic acid in Escherichia coli. Membrane-associated sialytransferase complexes from Escherichia coli K-235 catalyze the incorporation of N-acetylneuraminic acid (NeuNAc) from cytidine 5-monophospho-N-acetylneuraminic acid (CMP-NeuNAc) into polymeric products and a lipid fraction. Reconstitution of enzyme activity in lipid-depleted membrane complexes had an absolute dependence on a purified phospholipid which was characterized by high resolution mass spectrometry following dephosphorylation as undecaprenol. An identical mass spectrum was obtained on the phosphorylated lipid confirming that the active derivative was undecaprenyl phosphate. This C55-isoprenoid alcohol accounted for 95% of the polyisoprenol and contained 11 isoprene units, each one unsaturated. The remaining 5% was composed of the C60 homologue, dodecaprenol. Ficarprenyl phosphate, an isomer of undecaprenyl phosphate, also restored enzymatic activity although on a molar basis, it was less active than undecaprenyl phosphate. These results provide direct evidence that sialyl polymer synthesis has an obligatory requirement for undecaprenyl phosphate, a membrane-bound lipid coenzyme which functions as an intermediate carrier of glycosyl residues in the biosynthesis of a variety of microbial cell surface polysaccharides. These data extend further the general class of polysaccharides whose synthesis involves undecaprenyl phosphate and are in accord with the hypothesis that this lipid acts as an intermediate carrier of sialyl residues in sialyl polymer synthesis according to the following reaction: CMP-NeuNAc plus P-undecaprenol in equilibrium NeuNAc-P-undecaprenol plus CMP Although the sialylated lipid remains to be characterized, evidence in support of this conclusion was obtained by kinetic analysis of N-acetylneuraminic acid transfer into the lipid soluble fraction and sialyl polymers. These studies showed a rapid incorporation of N-acetylneuraminic acid into the lipid-soluble fraction prior to maximal sialyl polymer formation, an observation consistent with a possible precursortion, an observation consistent with a possible precursor-product relationship. Confirmation that the radioactivity in the lipid-soluble fraction was lipid-linked N-acetylneuraminic acid was provided by the demonstration of the formation of a transitory sialyl-lipid with chromatographic properties expected of sialyl-undecaprenyl phosphate. Three additional lines of investigation implicated a functional role for undecaprenyl phosphate in sialyl polymer synthesis..."}
{"id": "PMID:1095567", "title": "Properties of membrane-associated sialyltransferase of Escherichia coli.", "content": "Membrane-associated sialyltransferase complexes in Escherichia coli K-235 can be dissociated by lipid deletion and reassembled by the addition of undecaprenyl phosphate, a unique membrane-bound lipid coenzyme. Following disruption of the cells by pressure disintegration and centrifugal fractionation, the sialyltransferase activity is assocatied with both a \"particulate\" and \"soluble\" complex. Kinetic studies as well as sugar nucleotide, metal ion, pH, ammonium sulfate, and thiol reagent requirements showed these two complexes contained functionally identical enzymatic activities. Isopycnic sucrose density gradient centrifugation studies carried out on unfractionated total membranes established that these sialytransferase activities were associated with membrane hybrids composed of different relative amounts of inner and outer membranes. Enzyme localization studies employing DPNH oxidase, a marker for the inner membrane, and relative phospholipid to protein composition determinations in the two complexes, provided added support for this conclusion. Sialyl polymer synthesis was not dependent on the incorporation of other monosaccharides and had no demonstrable metal ion requirement. Kinetic studies showed that the Km for cytidine 5-monophospho-N-acetylneuraminic acid in intact soluble and particulate enzyme preparations was 8.1 times 10-5M and 9.2 times 105M, respectively. Similarly, both enzyme complexes had nearly identical Vmax values. Following reassembly of delipidated enzyme preparations, however, there was a 10-fold increase in the Km value for the particulate enzyme and a 3-fold increase for the soluble enzyme. This increase was accompanied by an increase of approximately the same magnitude in the Vmax values. Since the lipid coenzyme was limiting in intact enzyme preparations, the increase in Vmax reflected an increase in the concentration of the active lipid in reconstituted complexes. Sialyl polymer synthesis in intact membrane preparations was stimulated by the exogenous addition of lipid. Insertion of the carrier lipid was dependent on temperature. At 37 degrees, a 120% increase in sialytransferase activity was observed while only a 35% increase was observed at 30 percent. At 20 degrees, no stimulation occurred. Fluidity of the lipid phase is apparently required for proper function of this membraneassociated enzyme complex. Thus, at 20 degrees, a temperature below the membrane lipid transition temperature, the lipids are relatively immobile.", "contents": "Properties of membrane-associated sialyltransferase of Escherichia coli. Membrane-associated sialyltransferase complexes in Escherichia coli K-235 can be dissociated by lipid deletion and reassembled by the addition of undecaprenyl phosphate, a unique membrane-bound lipid coenzyme. Following disruption of the cells by pressure disintegration and centrifugal fractionation, the sialyltransferase activity is assocatied with both a \"particulate\" and \"soluble\" complex. Kinetic studies as well as sugar nucleotide, metal ion, pH, ammonium sulfate, and thiol reagent requirements showed these two complexes contained functionally identical enzymatic activities. Isopycnic sucrose density gradient centrifugation studies carried out on unfractionated total membranes established that these sialytransferase activities were associated with membrane hybrids composed of different relative amounts of inner and outer membranes. Enzyme localization studies employing DPNH oxidase, a marker for the inner membrane, and relative phospholipid to protein composition determinations in the two complexes, provided added support for this conclusion. Sialyl polymer synthesis was not dependent on the incorporation of other monosaccharides and had no demonstrable metal ion requirement. Kinetic studies showed that the Km for cytidine 5-monophospho-N-acetylneuraminic acid in intact soluble and particulate enzyme preparations was 8.1 times 10-5M and 9.2 times 105M, respectively. Similarly, both enzyme complexes had nearly identical Vmax values. Following reassembly of delipidated enzyme preparations, however, there was a 10-fold increase in the Km value for the particulate enzyme and a 3-fold increase for the soluble enzyme. This increase was accompanied by an increase of approximately the same magnitude in the Vmax values. Since the lipid coenzyme was limiting in intact enzyme preparations, the increase in Vmax reflected an increase in the concentration of the active lipid in reconstituted complexes. Sialyl polymer synthesis in intact membrane preparations was stimulated by the exogenous addition of lipid. Insertion of the carrier lipid was dependent on temperature. At 37 degrees, a 120% increase in sialytransferase activity was observed while only a 35% increase was observed at 30 percent. At 20 degrees, no stimulation occurred. Fluidity of the lipid phase is apparently required for proper function of this membraneassociated enzyme complex. Thus, at 20 degrees, a temperature below the membrane lipid transition temperature, the lipids are relatively immobile."}
{"id": "PMID:1095568", "title": "Synthesis and turnover of basal level guanosine tetraphosphate in Escherichia coli.", "content": "Cultures of escherichia coli growing exponentially in Trisacetate medium were subjected to nutritional shift-up and the pool size of guanosine 5'-3'-diphosphate-3'diphosphate (ppGpp) as well as the rates of protein synthesis and net RNA synthesis were determined. In the shift to a rich medium (glucose plus 19 amino acids plus hypoxanthine) the basal level of ppGpp falls immediately with a decay constant suggesting total inhibition of synthesis; ther is no ppGpp detectable above background for 30 to 40 min. The net rate of RNA synthesis starts to increase within 1 min of the shift-up and has reached its definite postshift value well before the pool of ppGGpp rises above background lvel. In a shift-up from Tris-acetate medium to Tris-glucose medium there is a much slower readjustment of the ppGpp pool size without the transient disappearance of the nucleotide. However, in a shift-up to Tris-acetate plus 5 amino acids, a medium which supports the same growth rate as Tris-glucose medium, a dramatic, transient drop in the ppGpp pool level was observed. Relaxed cells exhibit very similar behavior to strigent cells in the same shift-up. Our data argue strongly against an exclusive role for pGpp in regulating RNA synthesis during niutritional shift-up. The kinetic data of [3H]guanosine uptake into GTP and ppGpp pools were analyzed to determine the rate of pGpp synthesis. This rate was found to be similar during expotential growth in either Tris-acetate medium. During a shift-down from Tris-glucose to Tris-acetate medium the rate of ppGpp syntesis fell by a factor of 1.5 to 2 and the turnover rate is reduced 6- to 8-fold, suggesting that the expansion in the ppGp pool during shift-down canot be due to derepression of synthesis.", "contents": "Synthesis and turnover of basal level guanosine tetraphosphate in Escherichia coli. Cultures of escherichia coli growing exponentially in Trisacetate medium were subjected to nutritional shift-up and the pool size of guanosine 5'-3'-diphosphate-3'diphosphate (ppGpp) as well as the rates of protein synthesis and net RNA synthesis were determined. In the shift to a rich medium (glucose plus 19 amino acids plus hypoxanthine) the basal level of ppGpp falls immediately with a decay constant suggesting total inhibition of synthesis; ther is no ppGpp detectable above background for 30 to 40 min. The net rate of RNA synthesis starts to increase within 1 min of the shift-up and has reached its definite postshift value well before the pool of ppGGpp rises above background lvel. In a shift-up from Tris-acetate medium to Tris-glucose medium there is a much slower readjustment of the ppGpp pool size without the transient disappearance of the nucleotide. However, in a shift-up to Tris-acetate plus 5 amino acids, a medium which supports the same growth rate as Tris-glucose medium, a dramatic, transient drop in the ppGpp pool level was observed. Relaxed cells exhibit very similar behavior to strigent cells in the same shift-up. Our data argue strongly against an exclusive role for pGpp in regulating RNA synthesis during niutritional shift-up. The kinetic data of [3H]guanosine uptake into GTP and ppGpp pools were analyzed to determine the rate of pGpp synthesis. This rate was found to be similar during expotential growth in either Tris-acetate medium. During a shift-down from Tris-glucose to Tris-acetate medium the rate of ppGpp syntesis fell by a factor of 1.5 to 2 and the turnover rate is reduced 6- to 8-fold, suggesting that the expansion in the ppGp pool during shift-down canot be due to derepression of synthesis."}
{"id": "PMID:1095569", "title": "The subunit structure of phosphoglucose isomerase from bakers' yeast.", "content": "Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding to one-half and one-fourth of the preparative molecular weight of 119,400 determined by equilibrium centrifugation. Further centrifugation studies showed that the enzyme could be completely dissociated to species of 30,000 molecular weight. Peptide maps of tryptic hydrolysates of denatured and chemically modified enzyme showed that the protein is composed of four identical or nearly identical sub-units. The results of amino acid analysis, except half-cystine content, were compatible with identical subunits. The appearent partial specific volume and extinction coefficient were also determined.", "contents": "The subunit structure of phosphoglucose isomerase from bakers' yeast. Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding to one-half and one-fourth of the preparative molecular weight of 119,400 determined by equilibrium centrifugation. Further centrifugation studies showed that the enzyme could be completely dissociated to species of 30,000 molecular weight. Peptide maps of tryptic hydrolysates of denatured and chemically modified enzyme showed that the protein is composed of four identical or nearly identical sub-units. The results of amino acid analysis, except half-cystine content, were compatible with identical subunits. The appearent partial specific volume and extinction coefficient were also determined."}
{"id": "PMID:1095570", "title": "Two photo-cross-linked complexes of isoleucine specific transfer ribonucleic acid with aminoacyl transfer ribonucleic acid synthetases.", "content": "Escherichia coli Ile-tRNA synthetase and tRNA-Ile have been cross-linked photochemically by the direct action of ultraviolet light. In addition, photo-induced joining of tRNA-Ile E. coli to Val-tRNA synthetase from yeast has also been achieved. This yeast enzyme is known to mischarge E. coli tRNA-Ile with valine. Regions on tRNA-Ile involved in cross-linking have been determined for both complexes. In each case, three distinct parts of the nucleic acid are found cross-linked. Two of these are the same in both complexes and involve the dihydrouridine stem and loop region. The third part is unique for each complex. It involves the 3' terminus in the cognate one and the 3' side of the anticodon in the non-cognate case. When the cross-linked regions are projected onto a model of the three-dimensional structure of tRNA, it is clear that these and other data are consistent with having each enzyme bound in a similar orientation across tRNA-Ile. The enzymes are viewed as spanning the distance from the anticodon to the 3' terminus and making extensive contact with the area in which the two helical branches of the L-shape tRNA structure come together.", "contents": "Two photo-cross-linked complexes of isoleucine specific transfer ribonucleic acid with aminoacyl transfer ribonucleic acid synthetases. Escherichia coli Ile-tRNA synthetase and tRNA-Ile have been cross-linked photochemically by the direct action of ultraviolet light. In addition, photo-induced joining of tRNA-Ile E. coli to Val-tRNA synthetase from yeast has also been achieved. This yeast enzyme is known to mischarge E. coli tRNA-Ile with valine. Regions on tRNA-Ile involved in cross-linking have been determined for both complexes. In each case, three distinct parts of the nucleic acid are found cross-linked. Two of these are the same in both complexes and involve the dihydrouridine stem and loop region. The third part is unique for each complex. It involves the 3' terminus in the cognate one and the 3' side of the anticodon in the non-cognate case. When the cross-linked regions are projected onto a model of the three-dimensional structure of tRNA, it is clear that these and other data are consistent with having each enzyme bound in a similar orientation across tRNA-Ile. The enzymes are viewed as spanning the distance from the anticodon to the 3' terminus and making extensive contact with the area in which the two helical branches of the L-shape tRNA structure come together."}
{"id": "PMID:1095571", "title": "Isolation of the alpha and beta subunits of Escherichia coli succinyl coenzyme A synthetase and their recombination into active enzyme.", "content": "Pure preparations of the alpha and beta subunits of succinyl-CoA synthetase of Escherichia coli have been obtained by preparative gel electrophoresis in the presence of detergent or by gel filtration in acid urea solution. Substantial enzyme activity (50 to 60%) can be recovered following renaturation of an equimolar mixture of the two subunits prepared by gel filtration. The substrate ATP is required for reconstitution of activity, but the presence of our substrates does not appear to influence the renaturation process. Knowing that ATP phosphorylates the alpha subunit in the native enzyme, these data suggest that phosphorylation of this subunit is necessary for correct assembly. Comparison of the amino acid compositions of the stoichiometry of the subunit reassociation in the reconstitution process confirm the alpha2 beta2 structure for the native enzyme.", "contents": "Isolation of the alpha and beta subunits of Escherichia coli succinyl coenzyme A synthetase and their recombination into active enzyme. Pure preparations of the alpha and beta subunits of succinyl-CoA synthetase of Escherichia coli have been obtained by preparative gel electrophoresis in the presence of detergent or by gel filtration in acid urea solution. Substantial enzyme activity (50 to 60%) can be recovered following renaturation of an equimolar mixture of the two subunits prepared by gel filtration. The substrate ATP is required for reconstitution of activity, but the presence of our substrates does not appear to influence the renaturation process. Knowing that ATP phosphorylates the alpha subunit in the native enzyme, these data suggest that phosphorylation of this subunit is necessary for correct assembly. Comparison of the amino acid compositions of the stoichiometry of the subunit reassociation in the reconstitution process confirm the alpha2 beta2 structure for the native enzyme."}
{"id": "PMID:1095572", "title": "Leucine transport in Escherichia coli. The resolution of multiple transport systems and their coupling to metabolic energy.", "content": "The multiple active transport systems mediating L-leucine accumulation in Escherichia coli strain 7 (K12) and ML 308-225 have been examined. In addition to the previously characterized osmotic shock-sensitive LS (L-leucine-specific) and LIV-I (L-leucine; L-isoleucine-, and L-valine-specific) activities, a third system (designated LIV-II) has been detected, confirming a report by Rahmanian et al. (RAHMANIAN, M., CLAUS, D.R., and OXENDER, D. L. (1973) J. Bacteriol. 116, 1258-1266). This third system transports L-leucine, L-isoleucine, and L-valine with a relatively low affinity (apparent transport Km equals 4 muM for L-leucine) and it is resistant to repression by cell growth on L-leucine. Exploitation of these properties and of the differential sensitivity of the three transport activities to inhibition by L-leucine analogues permits estimation of the contribution by each system to the total transport activity under varying conditions. Such experiments show that, unlike systems LS and LIV-I, system LIV-II is resistant to osmotic shock. The L-leucine, L-iosleucine, and L-valine transport activity in membrane vesicles from strain ML 308-225 has the properties of system LIV-II. Although the L-leucine transport activities in strains 7 and ML 308-225 are in all other respects similar, membrane vesicles from strain 7 do not transport L-leucine, L-isoleucine, or L-valine. L-leucine transport under various conditions of energy supply has been measured in strain ML 308-225 and the corresponding Mg-2+-ATP-ASE-DEFICIENT STRAIN, DL-54. These measurements support the view that the osmotic shock-sensitive LS and LIV-I activities depend on the synthesis of ATP, while the osmotic shock-resistant LIV-II activity depends on the energized membrane state generated by electron flow but not on ATP synthesis, per se. This conclusion is not supported by the inhibitory effects of the energy poisons arsenate and 2,4-dinitrophenol, but these compounds may have secondary chemical effects on the transport systems.", "contents": "Leucine transport in Escherichia coli. The resolution of multiple transport systems and their coupling to metabolic energy. The multiple active transport systems mediating L-leucine accumulation in Escherichia coli strain 7 (K12) and ML 308-225 have been examined. In addition to the previously characterized osmotic shock-sensitive LS (L-leucine-specific) and LIV-I (L-leucine; L-isoleucine-, and L-valine-specific) activities, a third system (designated LIV-II) has been detected, confirming a report by Rahmanian et al. (RAHMANIAN, M., CLAUS, D.R., and OXENDER, D. L. (1973) J. Bacteriol. 116, 1258-1266). This third system transports L-leucine, L-isoleucine, and L-valine with a relatively low affinity (apparent transport Km equals 4 muM for L-leucine) and it is resistant to repression by cell growth on L-leucine. Exploitation of these properties and of the differential sensitivity of the three transport activities to inhibition by L-leucine analogues permits estimation of the contribution by each system to the total transport activity under varying conditions. Such experiments show that, unlike systems LS and LIV-I, system LIV-II is resistant to osmotic shock. The L-leucine, L-iosleucine, and L-valine transport activity in membrane vesicles from strain ML 308-225 has the properties of system LIV-II. Although the L-leucine transport activities in strains 7 and ML 308-225 are in all other respects similar, membrane vesicles from strain 7 do not transport L-leucine, L-isoleucine, or L-valine. L-leucine transport under various conditions of energy supply has been measured in strain ML 308-225 and the corresponding Mg-2+-ATP-ASE-DEFICIENT STRAIN, DL-54. These measurements support the view that the osmotic shock-sensitive LS and LIV-I activities depend on the synthesis of ATP, while the osmotic shock-resistant LIV-II activity depends on the energized membrane state generated by electron flow but not on ATP synthesis, per se. This conclusion is not supported by the inhibitory effects of the energy poisons arsenate and 2,4-dinitrophenol, but these compounds may have secondary chemical effects on the transport systems."}
{"id": "PMID:1095573", "title": "Transport in an Escherichia coli fatty acid auxotroph. A novel case of catabolite repression.", "content": "The transport of alpha-methylglucoside and a variety of amino acids including histidine, leucine, lysine, proline, and threonine are reduced 5- to 50-fold in Escherichia coli K1061, an unsaturated fatty acid auxotroph, when cells are grown in medium containing glucose as a carbon source and supplemented with either cis-vaccenic acid or cis-9, 10-methylenehexadecanoic acid. Cells grown in glucose medium containing oleate, palmitoleate, or cis-7-hexadecenoate do not exhibit this effect and transport alpha-methylglucoside and amino acids at rates comparable to those of a wild type strain. For cis-vaccenate- and cis-9, 10-methylenehexadecanoate-grown cells the capacity to transport amino acids and alpha-methylglucoside is normal when glycerol or succinate is the energy supply or when cyclic adenosine 3':5'-monophosphate is added to cultures growing on glucose. These results suggest that in this strain several transport systems are affected by catabolite repression, but the phenomenon is unique since repression requires not only glucose but specific unsaturated fatty acids, cis-vaccenic acid, or cis-9, 20-methylenehexadecanoic acid.", "contents": "Transport in an Escherichia coli fatty acid auxotroph. A novel case of catabolite repression. The transport of alpha-methylglucoside and a variety of amino acids including histidine, leucine, lysine, proline, and threonine are reduced 5- to 50-fold in Escherichia coli K1061, an unsaturated fatty acid auxotroph, when cells are grown in medium containing glucose as a carbon source and supplemented with either cis-vaccenic acid or cis-9, 10-methylenehexadecanoic acid. Cells grown in glucose medium containing oleate, palmitoleate, or cis-7-hexadecenoate do not exhibit this effect and transport alpha-methylglucoside and amino acids at rates comparable to those of a wild type strain. For cis-vaccenate- and cis-9, 10-methylenehexadecanoate-grown cells the capacity to transport amino acids and alpha-methylglucoside is normal when glycerol or succinate is the energy supply or when cyclic adenosine 3':5'-monophosphate is added to cultures growing on glucose. These results suggest that in this strain several transport systems are affected by catabolite repression, but the phenomenon is unique since repression requires not only glucose but specific unsaturated fatty acids, cis-vaccenic acid, or cis-9, 20-methylenehexadecanoic acid."}
{"id": "PMID:1095574", "title": "Tryptophan operon read-through. Isolation and characterization of an abnormally long tryptophan synthetase alpha subunit from a frame-shift mutant of Escherichia coli.", "content": "A new mutant strain of Escherichia coli, strain ICR-47, contains a frame-shift mutation in the trpA gene, the gene most distal to the operator in the trp operon. Mapping experiments indicate that the lesion is located at a site within 10 to 15% of the end of this gene. The mutation results in \"out-of-phase\" translation of the distal portion of the trp mRNA; normal translational termination signal(s) are not encountered and a trpA gene product longer than the wild type protein is produced. As with the other enzymes produced from this operon, the in vivo level of the altered protein (the alpha subunit of the tryptophan synthetase enzyme complex) is controlled by exogenous L-tryptophan. The altered alpha subunit from the strain ICR-47 has been isolated and characterized. Molecular weight estimations indicate a molecular weight of approximately 37,000, an increase beyond the wild type enzyme corresponding to an additional 50 to 70 amino acid residues. The protein has a new COOH-terminal amino acid sequence. Results of preliminary hybridization experiments suggest that the ICR-47 mRNA, which is necessarily longer than that needed to code for wild type enzyme, is not detectably different in size from wild type mRNA. The enzymatic properties of the ICR-47 alpha subunit indicates a greatly reduced ability of the mutant subunit to combine functionally with wild type beta2 subunit, the second protein component in the tryptophan synthetase enzyme complex. In contrast, only 40 to 50% of the intrinsic enzymatic activity of the alpha subunit is lost.", "contents": "Tryptophan operon read-through. Isolation and characterization of an abnormally long tryptophan synthetase alpha subunit from a frame-shift mutant of Escherichia coli. A new mutant strain of Escherichia coli, strain ICR-47, contains a frame-shift mutation in the trpA gene, the gene most distal to the operator in the trp operon. Mapping experiments indicate that the lesion is located at a site within 10 to 15% of the end of this gene. The mutation results in \"out-of-phase\" translation of the distal portion of the trp mRNA; normal translational termination signal(s) are not encountered and a trpA gene product longer than the wild type protein is produced. As with the other enzymes produced from this operon, the in vivo level of the altered protein (the alpha subunit of the tryptophan synthetase enzyme complex) is controlled by exogenous L-tryptophan. The altered alpha subunit from the strain ICR-47 has been isolated and characterized. Molecular weight estimations indicate a molecular weight of approximately 37,000, an increase beyond the wild type enzyme corresponding to an additional 50 to 70 amino acid residues. The protein has a new COOH-terminal amino acid sequence. Results of preliminary hybridization experiments suggest that the ICR-47 mRNA, which is necessarily longer than that needed to code for wild type enzyme, is not detectably different in size from wild type mRNA. The enzymatic properties of the ICR-47 alpha subunit indicates a greatly reduced ability of the mutant subunit to combine functionally with wild type beta2 subunit, the second protein component in the tryptophan synthetase enzyme complex. In contrast, only 40 to 50% of the intrinsic enzymatic activity of the alpha subunit is lost."}
{"id": "PMID:1095575", "title": "Turnover of protein synthetic elongation and initiation factors in Escherichia coli.", "content": "In an effort to understand the regulation of expression of the factors required for protein biosynthesis, we have measured the turnover of elongation factor G (EF-G) and initiation factor 2 (IF-2). Using a quantitative assay which involves immunoprecipitation and gel electrophoresis, it was found that both of these factors are very stable cellular proteins, but that their rates of accumulation are quite different, indicating a differential rate of synthesis. The effects of amino acid starvation and the relaxed response were also examined. The results showed that under these conditions EF-G and IF-2 are neither synthesized nor degraded.", "contents": "Turnover of protein synthetic elongation and initiation factors in Escherichia coli. In an effort to understand the regulation of expression of the factors required for protein biosynthesis, we have measured the turnover of elongation factor G (EF-G) and initiation factor 2 (IF-2). Using a quantitative assay which involves immunoprecipitation and gel electrophoresis, it was found that both of these factors are very stable cellular proteins, but that their rates of accumulation are quite different, indicating a differential rate of synthesis. The effects of amino acid starvation and the relaxed response were also examined. The results showed that under these conditions EF-G and IF-2 are neither synthesized nor degraded."}
{"id": "PMID:1095576", "title": "Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor.", "content": "RNA transcripts were synthesized in vitro from a lambda DNA template with purified Escherichia coli RNA polymerase either in the presence or absence of the protein termination factor, rho. The products were initially characterized by electrophoresis on polyacrylamide slab gels, and two of the lower molecular weight discrete species (6 S and 4 S RNA) were further characterized by standard two-dimensional \"fingerprint\" analysis. Production of the 4 S RNA was strongly affected by the presence of rho, whereas production of the 6 S RNA species was relatively unaffected by rho. 3'-Terminal oligonucleotide fragments were then selectively isolated on columns of dihydroxyboryl-substituted cellulose from these transcripts. Sequence analysis of these oligonucleotide products indicated: (a) that all of the transcripts examined possess similar degrees of 3'-terminal sequence heterogeneity which consisted predominantly of the addition of 1 to 5 adenylate residues to the 3'-terminus of the transcript; and (b) that rho factor-enhanced termination results in a definite structural change in the nucleotide sequence with which an RNA molecule can terminate.", "contents": "Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor. RNA transcripts were synthesized in vitro from a lambda DNA template with purified Escherichia coli RNA polymerase either in the presence or absence of the protein termination factor, rho. The products were initially characterized by electrophoresis on polyacrylamide slab gels, and two of the lower molecular weight discrete species (6 S and 4 S RNA) were further characterized by standard two-dimensional \"fingerprint\" analysis. Production of the 4 S RNA was strongly affected by the presence of rho, whereas production of the 6 S RNA species was relatively unaffected by rho. 3'-Terminal oligonucleotide fragments were then selectively isolated on columns of dihydroxyboryl-substituted cellulose from these transcripts. Sequence analysis of these oligonucleotide products indicated: (a) that all of the transcripts examined possess similar degrees of 3'-terminal sequence heterogeneity which consisted predominantly of the addition of 1 to 5 adenylate residues to the 3'-terminus of the transcript; and (b) that rho factor-enhanced termination results in a definite structural change in the nucleotide sequence with which an RNA molecule can terminate."}
{"id": "PMID:1095577", "title": "Resolution of D-serine dehydratase by cysteine.  An analytical treatment.", "content": "A general method is presented for analysis of the resolution of pyridoxal-P-requiring enzymes by carbonyl reagents.  The method is useful for accurately determining the very small equilibrium constants (KP) which characterize the dissociation of cofactor from many pyridoxal-P-requiring enzymes.  The analysis also establishes the minimum number and relative stabilities of distinct enzymic species involved in the resolution process.  Analysis of the resolution of D-serine dehydratase by L-and D-cysteine resulted in the establishment of an enzyme bound thiazolidine derivative as an intermediate in the pathway for resolution.  The over-all equilibrium constant (KR) for the reaction, D-serine dehydratase + cystein in equilibrium KR thiazolidine derivative +D-serine apodehydratase was determined.  At pH 7.80, T/2 0.33, 25 degrees, KR equal to 1.08 times 10-minus 3.  A value of 7.0 nM for the equilibrium constant for the dissociation of D-serine dehydratase to apoenzyme and free pyridoxal-P was determined from the ratio KR/KT, where KT is the equilibrium constant for the formation of a thiazolidine derivative from free pyridoxal-P and cysteine.  An estimate of 14 nM for KP was also obtained from partial resolution of D-serine dehydratase by high dilution.  The difficulties associated with this direct determination of KP from the dependence on the enzyme concentration of the activity of very dilute solutions of enzyme are discussed.", "contents": "Resolution of D-serine dehydratase by cysteine.  An analytical treatment. A general method is presented for analysis of the resolution of pyridoxal-P-requiring enzymes by carbonyl reagents.  The method is useful for accurately determining the very small equilibrium constants (KP) which characterize the dissociation of cofactor from many pyridoxal-P-requiring enzymes.  The analysis also establishes the minimum number and relative stabilities of distinct enzymic species involved in the resolution process.  Analysis of the resolution of D-serine dehydratase by L-and D-cysteine resulted in the establishment of an enzyme bound thiazolidine derivative as an intermediate in the pathway for resolution.  The over-all equilibrium constant (KR) for the reaction, D-serine dehydratase + cystein in equilibrium KR thiazolidine derivative +D-serine apodehydratase was determined.  At pH 7.80, T/2 0.33, 25 degrees, KR equal to 1.08 times 10-minus 3.  A value of 7.0 nM for the equilibrium constant for the dissociation of D-serine dehydratase to apoenzyme and free pyridoxal-P was determined from the ratio KR/KT, where KT is the equilibrium constant for the formation of a thiazolidine derivative from free pyridoxal-P and cysteine.  An estimate of 14 nM for KP was also obtained from partial resolution of D-serine dehydratase by high dilution.  The difficulties associated with this direct determination of KP from the dependence on the enzyme concentration of the activity of very dilute solutions of enzyme are discussed."}
{"id": "PMID:1095578", "title": "Bacteriophage T7 Deoxyribonucleic acid replication in vitro.  A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity.", "content": "In vivo, replication of T7 DNA does not occur after infection of Escherchia coli tsnC mutants (CHAMBERLIN, M. (1974) J. Virol. 14, 509-516). In vitro, extracts of tsnC mutant E. coli infected with T7 hage are incapable of replicating duplex T7 DNA, although extracts of wild type E. coli infected with T7 phage support replication of T7 DNA. In addition, extracts of the infected tsnC mutant are deficient in T7 DNA polymerase activity. Extracts prepared from uninfected E.coli tsnC-+ cells restore the ability of the infected tsnC extracts to replicate duplex T7 DNA, and also restore normal levels of the phage DNA polymerase activity. A 12,000-dalton heat-stable protein responsible for this complementation has been purified to near homogeneity from uninfected tsnC+ extracts and it is designated \"TsnC protein.\"", "contents": "Bacteriophage T7 Deoxyribonucleic acid replication in vitro.  A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. In vivo, replication of T7 DNA does not occur after infection of Escherchia coli tsnC mutants (CHAMBERLIN, M. (1974) J. Virol. 14, 509-516). In vitro, extracts of tsnC mutant E. coli infected with T7 hage are incapable of replicating duplex T7 DNA, although extracts of wild type E. coli infected with T7 phage support replication of T7 DNA. In addition, extracts of the infected tsnC mutant are deficient in T7 DNA polymerase activity. Extracts prepared from uninfected E.coli tsnC-+ cells restore the ability of the infected tsnC extracts to replicate duplex T7 DNA, and also restore normal levels of the phage DNA polymerase activity. A 12,000-dalton heat-stable protein responsible for this complementation has been purified to near homogeneity from uninfected tsnC+ extracts and it is designated \"TsnC protein.\""}
{"id": "PMID:1095579", "title": "Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits.", "content": "The DNA polymerase induced after infection of Escherichia coli by phage T7 has been purified 500-fold to near homogeneity as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.  The purified enzyme complements extracts of cells infected with a T7 gene 5 mutant to permit cell-free replication of duplex T7 DNA.  In contrast, purified T4 DNA polymerase or E. coli DNA polymerase I is unable to do so, thus suggesting a specific requirement for the T7 enzyme in the replication of the viral DNA.  E. coli TsnC protein is present in purified T7 DNA polymerase in one-to-one stoichiometry with T7 gene 5 protein, and can be isolated in homogeneous form from heat-denatured enzyme by chromatography on DEAE-cellulose.  The inactive form of T7 gene 5 protein that accumulates in tsnC hosts has been partially purified.  When partially purified gene 5 protein is mixed with purified TsnC protein, DNA polymerase activity is restored, and formation of a one-to-one complex between the two proteins occurs.  These results indicate that the functional form ofT7 DNA polymerase is a complex composed of phage- and host-specified subunits.", "contents": "Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. The DNA polymerase induced after infection of Escherichia coli by phage T7 has been purified 500-fold to near homogeneity as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.  The purified enzyme complements extracts of cells infected with a T7 gene 5 mutant to permit cell-free replication of duplex T7 DNA.  In contrast, purified T4 DNA polymerase or E. coli DNA polymerase I is unable to do so, thus suggesting a specific requirement for the T7 enzyme in the replication of the viral DNA.  E. coli TsnC protein is present in purified T7 DNA polymerase in one-to-one stoichiometry with T7 gene 5 protein, and can be isolated in homogeneous form from heat-denatured enzyme by chromatography on DEAE-cellulose.  The inactive form of T7 gene 5 protein that accumulates in tsnC hosts has been partially purified.  When partially purified gene 5 protein is mixed with purified TsnC protein, DNA polymerase activity is restored, and formation of a one-to-one complex between the two proteins occurs.  These results indicate that the functional form ofT7 DNA polymerase is a complex composed of phage- and host-specified subunits."}
{"id": "PMID:1095580", "title": "Bacteriophage T7 deoxyribonucleic acid replication in vitro.  Purification and properties of the gene 4 protein of bacteriophage T7.", "content": "The T7 gene 4 protein, a protein known from genetic analysis to participate in phage DNA replication in vivo, has been purified approximately 500-fold with an in vitro complementation assay. The protein, purified from cells infected with a T7 gene 4 temperature-sensitive mutant, is thermolabile, establishing that the complementation activity is in the protein product of the phage gene 4. The purified protein has no detectable nuclease, DNA polymerase, or RNA polymerase activity. However, in addition to stimulating the rate of DNA replication in crude extracts of T7 gene 4 mutant-infected cells, the gene 4 protein effects a marked stimulation of DNA synthesis by the purified T7 DNA polymerase when duplex T7 DNA is used as template. This effect is not observed when denatured T7 DNA is used as template, or when phage T4 DNA polymerase or Escherichia coli DNA polymerase I, II, OR III is substituted for the T4 enzyme. Analysis of the DNA synthesized by the T7 DNA polymerase in the presence of the gene 4 protein indicates that much of the product is in short DNA chains which are not covalently attached to the template. This result suggests a novel mechanism for the initiation of DNA chains in this reaction.", "contents": "Bacteriophage T7 deoxyribonucleic acid replication in vitro.  Purification and properties of the gene 4 protein of bacteriophage T7. The T7 gene 4 protein, a protein known from genetic analysis to participate in phage DNA replication in vivo, has been purified approximately 500-fold with an in vitro complementation assay. The protein, purified from cells infected with a T7 gene 4 temperature-sensitive mutant, is thermolabile, establishing that the complementation activity is in the protein product of the phage gene 4. The purified protein has no detectable nuclease, DNA polymerase, or RNA polymerase activity. However, in addition to stimulating the rate of DNA replication in crude extracts of T7 gene 4 mutant-infected cells, the gene 4 protein effects a marked stimulation of DNA synthesis by the purified T7 DNA polymerase when duplex T7 DNA is used as template. This effect is not observed when denatured T7 DNA is used as template, or when phage T4 DNA polymerase or Escherichia coli DNA polymerase I, II, OR III is substituted for the T4 enzyme. Analysis of the DNA synthesized by the T7 DNA polymerase in the presence of the gene 4 protein indicates that much of the product is in short DNA chains which are not covalently attached to the template. This result suggests a novel mechanism for the initiation of DNA chains in this reaction."}
{"id": "PMID:1095581", "title": "Purification and characterization of homogeneous initiation factor M2A from rabbit reticulocytes.", "content": "Rabbit reticulocyte initiation factor M2A has been prepared in homogeneous form.  The final preparation was purified 2,300-fold and ran as a single band on polyacrylamide gel electrophoresis in three different buffer systems: alkaline, sodium dodecyl sulfate, and acidic 6.5 M urea.  IF-M2A also ran as a single band in polyacrylamide gel isoelectric focusing experiments with an apparent pI of 6.45.  The molecular weight of IF-M2A was approximately 125,000 based on determinations by low speed equilibrium centrifugation (118,000), sodium dodecyl sulfate gel electrophoresis (130,000) and s20,w combined with Stokes radius (124,000).  The amino acid composition of IF-M2A revealed three unusual features: a) the basic amino acids represented 19.4 mol %; b) glutamicacid (plus glutamine) constituted 18.8 mol%; c) tryptophan and cysteine residues were only 0.4 and 0.7 mol%, respectively.  Homogeneous IF-M2A was tested in several initiation assays using either natural or artificial mRNAs.  In each assay tested, homogeneous IF-M2A fully substituted for cruder preparations and at concentrations commensurate with its increased purity.  IF-M2A was also examined for ribosome-dependent GTP hydrolysis, an assay requiring ribosomes but no other initiation factors.  Analysis of the data yielded a Km for GTP of 10 muM and a Vmax for hydrolysis of 1.20pmol/mug IF-M2A/min.  In addition, IF-M2A mediated GTP hydrolysis required both 40 S and 60 S subunits for maximal activity.  The possibility that IF-M2A is a factor required for the joining of 40 S and 60 S subunits is discussed.", "contents": "Purification and characterization of homogeneous initiation factor M2A from rabbit reticulocytes. Rabbit reticulocyte initiation factor M2A has been prepared in homogeneous form.  The final preparation was purified 2,300-fold and ran as a single band on polyacrylamide gel electrophoresis in three different buffer systems: alkaline, sodium dodecyl sulfate, and acidic 6.5 M urea.  IF-M2A also ran as a single band in polyacrylamide gel isoelectric focusing experiments with an apparent pI of 6.45.  The molecular weight of IF-M2A was approximately 125,000 based on determinations by low speed equilibrium centrifugation (118,000), sodium dodecyl sulfate gel electrophoresis (130,000) and s20,w combined with Stokes radius (124,000).  The amino acid composition of IF-M2A revealed three unusual features: a) the basic amino acids represented 19.4 mol %; b) glutamicacid (plus glutamine) constituted 18.8 mol%; c) tryptophan and cysteine residues were only 0.4 and 0.7 mol%, respectively.  Homogeneous IF-M2A was tested in several initiation assays using either natural or artificial mRNAs.  In each assay tested, homogeneous IF-M2A fully substituted for cruder preparations and at concentrations commensurate with its increased purity.  IF-M2A was also examined for ribosome-dependent GTP hydrolysis, an assay requiring ribosomes but no other initiation factors.  Analysis of the data yielded a Km for GTP of 10 muM and a Vmax for hydrolysis of 1.20pmol/mug IF-M2A/min.  In addition, IF-M2A mediated GTP hydrolysis required both 40 S and 60 S subunits for maximal activity.  The possibility that IF-M2A is a factor required for the joining of 40 S and 60 S subunits is discussed."}
{"id": "PMID:1095582", "title": "Escherichia coli succinic thiolinase. Stoichiometry of phosphorylation and coenzyme A binding.", "content": "Equilibrium and covalent binding studies of succinic thiokinase from Escherichia coli indicates that there can be a stoichiometric relationship between coenzyme A binding and the phosphoyrlation capacity of the enzyme. A comparison of homogeneous enzyme preparations has revealed that enzyme of high specific activity exhibits greater binding capacity and that this property is proportional to enzyme activity. Phosphorylation capacity was related to specific activity in highly active enzyme preparations, but leveled off at 1 mol of phosphorus/mol of thiokinase. These studies show that the \"dimer of dimers\" structure of succinic thiokinase contains the expected two active sites and that this enzyme does not demonstrate \"half-the-sites\" reactivity. A coenzyme A binding  site of lower affinity can be detected in some enzyme samples of lower specific activity. Binding of coenzyme A to the higher affinity sites may involve positive cooperativity. ATP, unlike ADP, does not bind to phosphorylated enzyme.", "contents": "Escherichia coli succinic thiolinase. Stoichiometry of phosphorylation and coenzyme A binding. Equilibrium and covalent binding studies of succinic thiokinase from Escherichia coli indicates that there can be a stoichiometric relationship between coenzyme A binding and the phosphoyrlation capacity of the enzyme. A comparison of homogeneous enzyme preparations has revealed that enzyme of high specific activity exhibits greater binding capacity and that this property is proportional to enzyme activity. Phosphorylation capacity was related to specific activity in highly active enzyme preparations, but leveled off at 1 mol of phosphorus/mol of thiokinase. These studies show that the \"dimer of dimers\" structure of succinic thiokinase contains the expected two active sites and that this enzyme does not demonstrate \"half-the-sites\" reactivity. A coenzyme A binding  site of lower affinity can be detected in some enzyme samples of lower specific activity. Binding of coenzyme A to the higher affinity sites may involve positive cooperativity. ATP, unlike ADP, does not bind to phosphorylated enzyme."}
{"id": "PMID:1095583", "title": "Inhibition of thymidylate synthetase and dihydrofolate reductase by naturally occurring oligoglutamate derivatives of folic acid.", "content": "Naturally occurring oligoglutamate derivatives of folic acid in extracts of Escherichia coli have been isolated on the basis of their inhibitory actions toward thymidylate synthetase and dihydrofolate reductase. The inhibitor of thymidylate synthetase has been identified as N-5-formyl-H4pteroyloligoglutamate (approximately 5 amino acid residues). It is 150-fold more inhibitory than the monoglutamate. Synthetic N-5-formyl derivatives containing 3 to 6 glutamyl residues were prepared and found to be 67- to 200-fold more inhibitory than the monoglutamate. N-5-Formimino-H4pteroyltriglutamate is one-twentieth as inhibitory as the corresponding N-5-formyl derivative. The inhibitor of mouse leukemia dihydrofolate reductase has been identified as N-10-formylpteropentaglutamate. It is approximately 7 times as inhibitory as N-10-formylpteroylmonoglutamate. It is 4,400 times as inhibitory toward mouse leukemia dihydrofolate reductase compared with the enzyme from E. coli. Lysine analogs of N-5-formyl-H4folate containing alpha0glutamyl groups in peptide linkage to the epsilon-amino group of lysine were relatively poor inhibitors of thymidylate synthetase. The inhibitory action of folic acid oligoglutamates on E. coli thymidylate synthetase was subject to reversal with 0.4 M NaCl, an effect that was more marked with various pteroyloligoglutamates than with H4homopteroylmonoglutamate and N-5, N-8-deaza-N-10-methylpteroylmonoglutamate.", "contents": "Inhibition of thymidylate synthetase and dihydrofolate reductase by naturally occurring oligoglutamate derivatives of folic acid. Naturally occurring oligoglutamate derivatives of folic acid in extracts of Escherichia coli have been isolated on the basis of their inhibitory actions toward thymidylate synthetase and dihydrofolate reductase. The inhibitor of thymidylate synthetase has been identified as N-5-formyl-H4pteroyloligoglutamate (approximately 5 amino acid residues). It is 150-fold more inhibitory than the monoglutamate. Synthetic N-5-formyl derivatives containing 3 to 6 glutamyl residues were prepared and found to be 67- to 200-fold more inhibitory than the monoglutamate. N-5-Formimino-H4pteroyltriglutamate is one-twentieth as inhibitory as the corresponding N-5-formyl derivative. The inhibitor of mouse leukemia dihydrofolate reductase has been identified as N-10-formylpteropentaglutamate. It is approximately 7 times as inhibitory as N-10-formylpteroylmonoglutamate. It is 4,400 times as inhibitory toward mouse leukemia dihydrofolate reductase compared with the enzyme from E. coli. Lysine analogs of N-5-formyl-H4folate containing alpha0glutamyl groups in peptide linkage to the epsilon-amino group of lysine were relatively poor inhibitors of thymidylate synthetase. The inhibitory action of folic acid oligoglutamates on E. coli thymidylate synthetase was subject to reversal with 0.4 M NaCl, an effect that was more marked with various pteroyloligoglutamates than with H4homopteroylmonoglutamate and N-5, N-8-deaza-N-10-methylpteroylmonoglutamate."}
{"id": "PMID:1095584", "title": "Polyadenylate metabolism in the nuclei and cytoplasm of Saccharomyces cerevisiae.", "content": "A procedure has been designed for the simultaneous isolation, in a single step, of the nuclei and cytoplasm of Saccharomyces cerevisiae alphas288c spheroplasts. We have examined the polyadenylate poly(A)-containing RNA in these fractions and their kinetics of synthesis. Nuclear RNA saturates with [3H] adenine within 10 min. Labeled RNA appears very quickly in the cytoplasm, exceeding the amount of labeled nuclear RNA within 2 min after the addition of [3H] adenine. Nuclear poly(A)-containing RNA is approximately 10% of the total cellular poly(A)-containing RNA obtained from spheroplasts labeled for 15 min. Nuclear poly(A)-containing RNA is not as large as the giant heterogeneous nuclear RNA of animal cells. The distribution of molecular size in nuclear and cytoplasmic populations of poly(A)-containing RNA is very broad with the average size of the nuclear species being moderately larger than the cytoplasmic species. Three distinct size classes of poly(A), with different apparent kinetic properties, are obtained from yeast poly(A)-containing RNA. Their electrophoretic mobility suggests molecular lengths of approximately 20, 40, and 60 nucleotides (Groner, G., Hynes, N., and Phillips, S. (1975) Biochemistry, 13, 5378-5383). Each of these poly(A) classes are present in the mRNA from large and small polyribosomes.", "contents": "Polyadenylate metabolism in the nuclei and cytoplasm of Saccharomyces cerevisiae. A procedure has been designed for the simultaneous isolation, in a single step, of the nuclei and cytoplasm of Saccharomyces cerevisiae alphas288c spheroplasts. We have examined the polyadenylate poly(A)-containing RNA in these fractions and their kinetics of synthesis. Nuclear RNA saturates with [3H] adenine within 10 min. Labeled RNA appears very quickly in the cytoplasm, exceeding the amount of labeled nuclear RNA within 2 min after the addition of [3H] adenine. Nuclear poly(A)-containing RNA is approximately 10% of the total cellular poly(A)-containing RNA obtained from spheroplasts labeled for 15 min. Nuclear poly(A)-containing RNA is not as large as the giant heterogeneous nuclear RNA of animal cells. The distribution of molecular size in nuclear and cytoplasmic populations of poly(A)-containing RNA is very broad with the average size of the nuclear species being moderately larger than the cytoplasmic species. Three distinct size classes of poly(A), with different apparent kinetic properties, are obtained from yeast poly(A)-containing RNA. Their electrophoretic mobility suggests molecular lengths of approximately 20, 40, and 60 nucleotides (Groner, G., Hynes, N., and Phillips, S. (1975) Biochemistry, 13, 5378-5383). Each of these poly(A) classes are present in the mRNA from large and small polyribosomes."}
{"id": "PMID:1095585", "title": "The 30 S ribosomal precursor RNA from Escherichia coli.  A primary transcript containing 23 S, 16 S, and 5 S sequences.", "content": "The 30 S ribosomal precursor RNA has been prepared from Escherichia coli AB301/105 (RNase III-) labeled with 32-PO4 in the presence of chloramphenicol. Direct nucleotide sequence studies yield the following information. 1. The major 5'-terminal sequence in our precursor preparations is pppA-C-U-G-. 2. Treatment of the precursor RNA with purified ribonuclease III in vitro releases species sedimenting near 23 S and 17 S, neither of which retain the pppA- end, plus a collection of small fragments with chain lengths of less than 400 nucleotides. 3. The RNase III product sedimenting near 17 S (16 SIII) appears identical with the 17 S RNA typically isolated from pulse-labeled or chloramphenicol-treated cells or from several mutants deficient in ribosome assembly: fingerprint analysis reveals the presence of the same additional RNase T1 oligonucleotides and the 5' terminus (pU-G-) previously described for 17 S RNA. A 3'-terminal T1 oligonucleotide (which was not previously identifiable in the case of the 17 S precursor) has been isolated from 16 S III and its sequence determined: C-U-C-A-C-A-C-A. 4. 5 S rRNA sequences are contained in an RNase III-released fragment of approximately 300 nucleotides. This molecule lacks the 5' terminus of the mature 5 S RNA. The implications of these findings with respect to the control of ribosomal RNA synthesis, the pathways of rRNA processing in vivo, and the specificity of RNase III cleavage of natural substrates are discussed.", "contents": "The 30 S ribosomal precursor RNA from Escherichia coli.  A primary transcript containing 23 S, 16 S, and 5 S sequences. The 30 S ribosomal precursor RNA has been prepared from Escherichia coli AB301/105 (RNase III-) labeled with 32-PO4 in the presence of chloramphenicol. Direct nucleotide sequence studies yield the following information. 1. The major 5'-terminal sequence in our precursor preparations is pppA-C-U-G-. 2. Treatment of the precursor RNA with purified ribonuclease III in vitro releases species sedimenting near 23 S and 17 S, neither of which retain the pppA- end, plus a collection of small fragments with chain lengths of less than 400 nucleotides. 3. The RNase III product sedimenting near 17 S (16 SIII) appears identical with the 17 S RNA typically isolated from pulse-labeled or chloramphenicol-treated cells or from several mutants deficient in ribosome assembly: fingerprint analysis reveals the presence of the same additional RNase T1 oligonucleotides and the 5' terminus (pU-G-) previously described for 17 S RNA. A 3'-terminal T1 oligonucleotide (which was not previously identifiable in the case of the 17 S precursor) has been isolated from 16 S III and its sequence determined: C-U-C-A-C-A-C-A. 4. 5 S rRNA sequences are contained in an RNase III-released fragment of approximately 300 nucleotides. This molecule lacks the 5' terminus of the mature 5 S RNA. The implications of these findings with respect to the control of ribosomal RNA synthesis, the pathways of rRNA processing in vivo, and the specificity of RNase III cleavage of natural substrates are discussed."}
{"id": "PMID:1095586", "title": "Stereochemical course of the adenosine triphosphate phosphoribosyltransferase reaction in histidine biosynthesis.", "content": "The product of the first reaction in histidine biosynthesis is shown by optical rotation measurements on three derivatives to have inverted, beta stereochemistry at the newly formed bond. This is in contrast to alpha linkage expected on the basis of previously observed exchange, specificity, and covalent intermediate phenomena. The postulate double displacement mechanism for adenosine triphosphate phosphoribosyltransferase must be modified to account for the product stereochemistry.", "contents": "Stereochemical course of the adenosine triphosphate phosphoribosyltransferase reaction in histidine biosynthesis. The product of the first reaction in histidine biosynthesis is shown by optical rotation measurements on three derivatives to have inverted, beta stereochemistry at the newly formed bond. This is in contrast to alpha linkage expected on the basis of previously observed exchange, specificity, and covalent intermediate phenomena. The postulate double displacement mechanism for adenosine triphosphate phosphoribosyltransferase must be modified to account for the product stereochemistry."}
{"id": "PMID:1095588", "title": "Acetabuloplasty in the treatment of congenital dislocation of the hip.", "content": "The results in 102 acetabuloplasties have been assessed in eighty-six patients, with a minimum follow-up of five years. Clinical and radiological assessment was carried out according to a score system very similar to that used in previous studies, and the results were expressed as excellent, good, fair and poor according to the score obtained. Any hip requiring further stabilising procedures was automatically grouped as a failure. Subtrochanteric femoral ostestomy was used when femoral anteversion was excessive. The results revealed that 73 per cent of the hips were satifactory (excellent or good) overall, but that a steady deterioration was evident when the five- and fifteen-year groups were compared. Few of the hips were regarded as normal on radiological grounds. Homologous bone bank rib was found to be the most satisfactory graft material. Coxa magna was the most common abnormality that was consistent with a good or excellent result. Avascular necrosis of the femoral head was found in only six hips; it was more commonly present in patients subjected to previous operation. Concentric reduction of the femoral head is essential if acetabuloplasty is to maintain stability of the hip.", "contents": "Acetabuloplasty in the treatment of congenital dislocation of the hip. The results in 102 acetabuloplasties have been assessed in eighty-six patients, with a minimum follow-up of five years. Clinical and radiological assessment was carried out according to a score system very similar to that used in previous studies, and the results were expressed as excellent, good, fair and poor according to the score obtained. Any hip requiring further stabilising procedures was automatically grouped as a failure. Subtrochanteric femoral ostestomy was used when femoral anteversion was excessive. The results revealed that 73 per cent of the hips were satifactory (excellent or good) overall, but that a steady deterioration was evident when the five- and fifteen-year groups were compared. Few of the hips were regarded as normal on radiological grounds. Homologous bone bank rib was found to be the most satisfactory graft material. Coxa magna was the most common abnormality that was consistent with a good or excellent result. Avascular necrosis of the femoral head was found in only six hips; it was more commonly present in patients subjected to previous operation. Concentric reduction of the femoral head is essential if acetabuloplasty is to maintain stability of the hip."}
{"id": "PMID:1095589", "title": "Quadrilateral shaped brims made from high-density polyethylene for long leg calipers.", "content": "Quadrilateral shaped brims made of high-density polyethylene have been used with satisfactory results on more than 120 patients who needed ischial-bearing long leg calipers. At first the fitting technique was the same as that used for thigh amputees. Subsequently it has been possible to fit most patients from a range of pre-formed brims. The properties of high-density polyethylene allow the caliper side irons to be riveted directly to the brim which, with the use of ready-made brims, allows a patient to be fitted rapidly with a comfortable and effective caliper.", "contents": "Quadrilateral shaped brims made from high-density polyethylene for long leg calipers. Quadrilateral shaped brims made of high-density polyethylene have been used with satisfactory results on more than 120 patients who needed ischial-bearing long leg calipers. At first the fitting technique was the same as that used for thigh amputees. Subsequently it has been possible to fit most patients from a range of pre-formed brims. The properties of high-density polyethylene allow the caliper side irons to be riveted directly to the brim which, with the use of ready-made brims, allows a patient to be fitted rapidly with a comfortable and effective caliper."}
{"id": "PMID:1095594", "title": "Reversible and permanent effects of the carbon sources and various antibiotics on the morphology and metabolic properties of Ustilago cynodontis cells.", "content": "The effects of various carbon sources and of antibiotics on the morphology of hypha cells of the fungus Ustilago cynodontis is described. Nonfermentable substrates promote readily reversible yeastlike colonies from hypha cells: all the hypha cells spread on these substrates give rise to yeastlike colonies that revert to the mycelial phenotype when transferred to glucose medium. Among the antibiotics tested, chloramphenicol (CAP) is found to promote, under certain circumstances, a long-lasting, even permanent modification on the morphology of the colonies: the colonies developed on CAP-glucose media are yeastlike, and a percentage of them give rise to colonies whose morphology remains yeastlike even on drug-free media: this effect is also obtained with cells cultivated in liquid medium. This permanent morphological modification is accompanied by a change of metabolic properties. Similar permanent effects are obtained with ethidium bromide, suggesting that mitochondrial functioning is involved in these modifications.", "contents": "Reversible and permanent effects of the carbon sources and various antibiotics on the morphology and metabolic properties of Ustilago cynodontis cells. The effects of various carbon sources and of antibiotics on the morphology of hypha cells of the fungus Ustilago cynodontis is described. Nonfermentable substrates promote readily reversible yeastlike colonies from hypha cells: all the hypha cells spread on these substrates give rise to yeastlike colonies that revert to the mycelial phenotype when transferred to glucose medium. Among the antibiotics tested, chloramphenicol (CAP) is found to promote, under certain circumstances, a long-lasting, even permanent modification on the morphology of the colonies: the colonies developed on CAP-glucose media are yeastlike, and a percentage of them give rise to colonies whose morphology remains yeastlike even on drug-free media: this effect is also obtained with cells cultivated in liquid medium. This permanent morphological modification is accompanied by a change of metabolic properties. Similar permanent effects are obtained with ethidium bromide, suggesting that mitochondrial functioning is involved in these modifications."}
{"id": "PMID:1095595", "title": "Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy.", "content": "Cells of many kinds adhere firmly to glass or plastic surfaces which have been pretreated with polylysine. The attachment takes place as soon as the cells make contact with the surfaces, and the flattening of the cells against the surfaces is quite rapid. Cells which do not normally adhere to solid surfaces, such as sea urchin eggs, attach as well as cells which normally do so, such as amebas or mammalian cells in culture. The adhesion is interpreted simply as the interaction between the polyanionic cell surfaces and the polycationic layer of adsorbed polylysine. The attachment of cells to the polylysine-treated surfaces can be exploited for a variety of experimental manipulations. In the preparation of samples for scanning or transmission electron microscopy, the living material may first be attached to a polylysine-coated plate or grid, subjected to some experimental treatment (fertilization of an egg, for example), then transferred rapidly to fixative and further  passed through processing for observation; each step involves only the transfer of the plate or grid from one container to the next. The cells are not detached. The adhesion of the cell may be so firm that the body of the cell may be sheared away, leaving attached a patch of cell surface, face up, for observation of its inner aspect. For example, one may observe secretory vesicles on the inner face of the surface (3) or may study the association of filaments with the inner surface (Fig. 1). Subcellular structures may attach to the polylysine-coated surfaces. So far, we have found this to be the case for nuclei isolated from sea urchin embryos and for the microtubules of flagella, which are well displayed after the membrane has been disrupted by Triton X-100 (Fig. 2).", "contents": "Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy. Cells of many kinds adhere firmly to glass or plastic surfaces which have been pretreated with polylysine. The attachment takes place as soon as the cells make contact with the surfaces, and the flattening of the cells against the surfaces is quite rapid. Cells which do not normally adhere to solid surfaces, such as sea urchin eggs, attach as well as cells which normally do so, such as amebas or mammalian cells in culture. The adhesion is interpreted simply as the interaction between the polyanionic cell surfaces and the polycationic layer of adsorbed polylysine. The attachment of cells to the polylysine-treated surfaces can be exploited for a variety of experimental manipulations. In the preparation of samples for scanning or transmission electron microscopy, the living material may first be attached to a polylysine-coated plate or grid, subjected to some experimental treatment (fertilization of an egg, for example), then transferred rapidly to fixative and further  passed through processing for observation; each step involves only the transfer of the plate or grid from one container to the next. The cells are not detached. The adhesion of the cell may be so firm that the body of the cell may be sheared away, leaving attached a patch of cell surface, face up, for observation of its inner aspect. For example, one may observe secretory vesicles on the inner face of the surface (3) or may study the association of filaments with the inner surface (Fig. 1). Subcellular structures may attach to the polylysine-coated surfaces. So far, we have found this to be the case for nuclei isolated from sea urchin embryos and for the microtubules of flagella, which are well displayed after the membrane has been disrupted by Triton X-100 (Fig. 2)."}
{"id": "PMID:1095596", "title": "Cytoplasmic activation of starfish oocytes by sperm and divalent ionophore A-23187.", "content": "The relationship between onset of the early cytoplasmic stages of oocyte activation (vitelline membrane separation and elevation) and nuclear meiotic maturation was investigated in starfish oocytes after their exposure to divalent ionophore (A-23187) or sperm. Meiotically mature oocytes, isolated in calcium-free seawater, underwent activation in response to sperm or ionophore as previously reported. Large, immature starfish oocytes, arrested in prophase I of meiosis (germinal vesicle stage), underwent vitelline membrane elevation when treated with divalent ionophore A-23187 or starfish sperm. Histological studies demonstrated that cortical granule breakdown in the oocyte cortex was associated with vitelline membrane elevation after these treatments. Activation of oocytes by sperm occurred only in response to starfish sperm. Sea urchin, sand dollar, surf clam, or marine worm sperm did not induce vitelline membrane elevation of either immature or mature starfish oocytes. Sperm- or ionophore-activated immature oocytes underwent nuclear maturation after addition of the meiosis-inducing hormone, l-methyladenine; however, parthenogenetic development did not occur and embryonic development was markedly inhibited. In contrast to previous studies, the present results indicate that cytoplasmic activation can be initiated before and without hormone induction of the nuclear maturation process. Differentiation of the oocyte cell surface or cortex reactivity therefore appears to occur during oogenesis rather than as a consequence of maturation. The data further support the view that divalent ions mediate certain of the early activation responses initiated by sperm at the time of fertilization and that synchronization of fertilization to the meiotic process in the oocyte is important for the occurrence of normal development.", "contents": "Cytoplasmic activation of starfish oocytes by sperm and divalent ionophore A-23187. The relationship between onset of the early cytoplasmic stages of oocyte activation (vitelline membrane separation and elevation) and nuclear meiotic maturation was investigated in starfish oocytes after their exposure to divalent ionophore (A-23187) or sperm. Meiotically mature oocytes, isolated in calcium-free seawater, underwent activation in response to sperm or ionophore as previously reported. Large, immature starfish oocytes, arrested in prophase I of meiosis (germinal vesicle stage), underwent vitelline membrane elevation when treated with divalent ionophore A-23187 or starfish sperm. Histological studies demonstrated that cortical granule breakdown in the oocyte cortex was associated with vitelline membrane elevation after these treatments. Activation of oocytes by sperm occurred only in response to starfish sperm. Sea urchin, sand dollar, surf clam, or marine worm sperm did not induce vitelline membrane elevation of either immature or mature starfish oocytes. Sperm- or ionophore-activated immature oocytes underwent nuclear maturation after addition of the meiosis-inducing hormone, l-methyladenine; however, parthenogenetic development did not occur and embryonic development was markedly inhibited. In contrast to previous studies, the present results indicate that cytoplasmic activation can be initiated before and without hormone induction of the nuclear maturation process. Differentiation of the oocyte cell surface or cortex reactivity therefore appears to occur during oogenesis rather than as a consequence of maturation. The data further support the view that divalent ions mediate certain of the early activation responses initiated by sperm at the time of fertilization and that synchronization of fertilization to the meiotic process in the oocyte is important for the occurrence of normal development."}
{"id": "PMID:1095597", "title": "Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs.", "content": "Receptors for Ricinus communis agglutinin I (RCAI), concanavalin A (Con A), and wheat germ agglutinin (WGA) were localized on the zonae pellucidae and plasma membranes of hamster, mouse, and rat eggs with ferritin-lectin conjugates. Intact eggs labeled with the ferritin conjugates showed dense concentrations of RCAI and WGA receptors in the outermost regions of their zonae pellucidae and sparse distributions of Con A receptors throughout the zonae. Ferritin-lectin labeling was specific, since inhibitory saccharides effectively blocked labeling. The asymmetric density of RCAI receptors across the zona was confirmed by ferritin-RCAI and fluorescein-RCAI labeling of mechanically isolated zonae pellucidae, indicating that the RCAI-binding sites are more densely distributed in the exterior zona regions. Plasma membranes of rodent eggs contained RCAI, WGA, and Con A receptors. These receptors were found to be more or less randomly distributed on surfaces of aldehyde-fixed eggs or on eggs labeled near 0 degrees C. However, eggs incubated at 25 degrees C showed aggregated WGA- and Con A-binding site distributions on their plasma membranes. This indicates that lectin-induced receptor redistribution occurs at this temperature. The possibility that plasma membrane receptor mobility is a requirement for sperm-egg fusion is discussed.", "contents": "Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs. Receptors for Ricinus communis agglutinin I (RCAI), concanavalin A (Con A), and wheat germ agglutinin (WGA) were localized on the zonae pellucidae and plasma membranes of hamster, mouse, and rat eggs with ferritin-lectin conjugates. Intact eggs labeled with the ferritin conjugates showed dense concentrations of RCAI and WGA receptors in the outermost regions of their zonae pellucidae and sparse distributions of Con A receptors throughout the zonae. Ferritin-lectin labeling was specific, since inhibitory saccharides effectively blocked labeling. The asymmetric density of RCAI receptors across the zona was confirmed by ferritin-RCAI and fluorescein-RCAI labeling of mechanically isolated zonae pellucidae, indicating that the RCAI-binding sites are more densely distributed in the exterior zona regions. Plasma membranes of rodent eggs contained RCAI, WGA, and Con A receptors. These receptors were found to be more or less randomly distributed on surfaces of aldehyde-fixed eggs or on eggs labeled near 0 degrees C. However, eggs incubated at 25 degrees C showed aggregated WGA- and Con A-binding site distributions on their plasma membranes. This indicates that lectin-induced receptor redistribution occurs at this temperature. The possibility that plasma membrane receptor mobility is a requirement for sperm-egg fusion is discussed."}
{"id": "PMID:1095598", "title": "Preparation and purification of polymerized actin from sea urchin egg extracts.", "content": "Isotonic extracts of the soluble cytoplasmic proteins of sea urchin eggs, containing sufficient EGTA to reduce the calcium concentration to low levels, form a dense gel on warming to 35-40 degrees C. Although this procedure is similar to that used to polymerize tubulin from mammalian brain, sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows this gel to have actin as a major component and to contain no tubulin. If such extracts are dialyzed against dilute salt solution, they no longer respond to warming, but gelation will occur if they are supplemented with 1 mM ATP and 0.020 M KCl before heating. Gelation is not temperature reversible, but the gelled material can be dissolved in 0.6-1 M KCl and these solutions contain F-actin filaments. These filaments slowly aggregate to microscopic, birefringent fibrils when  1 mM ATP is added to the solution, and this procedure provides a simple method for preparing purified actin. the supernate remaining after actin removal contains the other two components of the gel, proteins of approximately 58,000 and 220,000 mol wt. These two proteins plus actin recombine to form the original gel material when the ionic strength is reduced. This reaction is reversible at 0 degrees C, and no heating is required.", "contents": "Preparation and purification of polymerized actin from sea urchin egg extracts. Isotonic extracts of the soluble cytoplasmic proteins of sea urchin eggs, containing sufficient EGTA to reduce the calcium concentration to low levels, form a dense gel on warming to 35-40 degrees C. Although this procedure is similar to that used to polymerize tubulin from mammalian brain, sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows this gel to have actin as a major component and to contain no tubulin. If such extracts are dialyzed against dilute salt solution, they no longer respond to warming, but gelation will occur if they are supplemented with 1 mM ATP and 0.020 M KCl before heating. Gelation is not temperature reversible, but the gelled material can be dissolved in 0.6-1 M KCl and these solutions contain F-actin filaments. These filaments slowly aggregate to microscopic, birefringent fibrils when  1 mM ATP is added to the solution, and this procedure provides a simple method for preparing purified actin. the supernate remaining after actin removal contains the other two components of the gel, proteins of approximately 58,000 and 220,000 mol wt. These two proteins plus actin recombine to form the original gel material when the ionic strength is reduced. This reaction is reversible at 0 degrees C, and no heating is required."}
{"id": "PMID:1095599", "title": "The chondriome of selected trypanosomatids. A three-dimensional study based on serial thick sections and high voltage electron microscopy.", "content": "The unitary nature of the chondriome of two species of trypanosomatids, Blastocrithidia culicis and Trypanosoma cruzi, has been demonstrated by utilizing serial thick-sectioning techniques combined with high voltage electron microscopy. Profiles of mitochondrial elements seen in thin sections and suspected to be parts of a continuum were confirmed by serial thick sectioning (0.25-0.50 mum thick) and stereopair analysis to be parts of the same mitochondrion. Three-dimensional models obtained from tracings of mitochondrial profiles on cellulose acetate reveal the mitochondrion of B. culicis to consist of a posterior mass with six tubular extensions extending upward and terminating in the anterior apex. The kinetoplast was found suspended between two of the tubular extensions, or less frequently, protuding as a nodule from one of the extensions. A bifurcation of one of the extensions was found in some specimens. The mitochondrion of T. cruzi consists of a triangular-shaped convoluted tubule, the base being the kinetoplast portion while the apex is directed posteriorly. The mitochondrion bifurcates behind the flagellar pocket, lateral to the kinetoplast, sending two entwined extensions into the tenuous anterior apex. Whether the mitochondrion of T. cruzi is unitary in the trypomastigote form was not determined in this study, since only epimastigote forms were used.", "contents": "The chondriome of selected trypanosomatids. A three-dimensional study based on serial thick sections and high voltage electron microscopy. The unitary nature of the chondriome of two species of trypanosomatids, Blastocrithidia culicis and Trypanosoma cruzi, has been demonstrated by utilizing serial thick-sectioning techniques combined with high voltage electron microscopy. Profiles of mitochondrial elements seen in thin sections and suspected to be parts of a continuum were confirmed by serial thick sectioning (0.25-0.50 mum thick) and stereopair analysis to be parts of the same mitochondrion. Three-dimensional models obtained from tracings of mitochondrial profiles on cellulose acetate reveal the mitochondrion of B. culicis to consist of a posterior mass with six tubular extensions extending upward and terminating in the anterior apex. The kinetoplast was found suspended between two of the tubular extensions, or less frequently, protuding as a nodule from one of the extensions. A bifurcation of one of the extensions was found in some specimens. The mitochondrion of T. cruzi consists of a triangular-shaped convoluted tubule, the base being the kinetoplast portion while the apex is directed posteriorly. The mitochondrion bifurcates behind the flagellar pocket, lateral to the kinetoplast, sending two entwined extensions into the tenuous anterior apex. Whether the mitochondrion of T. cruzi is unitary in the trypomastigote form was not determined in this study, since only epimastigote forms were used."}
{"id": "PMID:1095600", "title": "Golgi organelle response to the antibiotic X537A.", "content": "The effects of the ionophoric antibiotic X537A on cell structure were studied with phase-contrast, fluorescence, and electron microscopy. X537A induced selective vacuolation of the Golgi apparatus of vascular and intestinal smooth muscle, epithelium, plasma cells, and cultured chick heart and guinea pig vascular smooth muscle cells. The swelling of the Golgi apparatus induced by X537A was reversible in the systems examined for reversibility: vascular smooth muscle and cultured chick heart. Myelin figures were common in the Golgi apparatus vacuolated by X537A. Fluorescence microscopy of cultured cells incubated with X537A showed the characteristic blue X537A fluorescence associated with lipid globules in the cultured cells. Incubation of cultured chick heart cells with X537A reduced the beating rate and, after 24-72 h, abolished the sarcomere pattern. The swelling of the Golgi membranes produced by X537A in cultured vascular smooth muscle was associated with inhibition of D-[6-3H]glucosamine and [35S]sulfate incorporation into glycosaminoglycans.", "contents": "Golgi organelle response to the antibiotic X537A. The effects of the ionophoric antibiotic X537A on cell structure were studied with phase-contrast, fluorescence, and electron microscopy. X537A induced selective vacuolation of the Golgi apparatus of vascular and intestinal smooth muscle, epithelium, plasma cells, and cultured chick heart and guinea pig vascular smooth muscle cells. The swelling of the Golgi apparatus induced by X537A was reversible in the systems examined for reversibility: vascular smooth muscle and cultured chick heart. Myelin figures were common in the Golgi apparatus vacuolated by X537A. Fluorescence microscopy of cultured cells incubated with X537A showed the characteristic blue X537A fluorescence associated with lipid globules in the cultured cells. Incubation of cultured chick heart cells with X537A reduced the beating rate and, after 24-72 h, abolished the sarcomere pattern. The swelling of the Golgi membranes produced by X537A in cultured vascular smooth muscle was associated with inhibition of D-[6-3H]glucosamine and [35S]sulfate incorporation into glycosaminoglycans."}
{"id": "PMID:1095601", "title": "Actin-like filaments in bone cells of cultured mouse calvaria as demonstrated by binding to heavy meromyosin.", "content": "A variety of intracellular filaments (50-70 A in diameter) found in bone cells was shown to bind specifically to HMM. Because of this property, these filaments are probably biochemically similar to muscle actin. in osteoblasts and osteocytes, these reactive filaments were oriented in bundles parallel to the plasma membrane and filling the cell processes. In the osteoclast the filaments along the cell membrane were not so highly organized. In the clear zone, the quiescent part of the cell adjacent to the motile ruffled border, organized filament bundles were oriented perpendicular to the cell membrane and terminated in short processes at the bone surface. These filaments were also reactive with HMM. The possible significance of the filaments with respect to the physiology of bone cells is discussed.", "contents": "Actin-like filaments in bone cells of cultured mouse calvaria as demonstrated by binding to heavy meromyosin. A variety of intracellular filaments (50-70 A in diameter) found in bone cells was shown to bind specifically to HMM. Because of this property, these filaments are probably biochemically similar to muscle actin. in osteoblasts and osteocytes, these reactive filaments were oriented in bundles parallel to the plasma membrane and filling the cell processes. In the osteoclast the filaments along the cell membrane were not so highly organized. In the clear zone, the quiescent part of the cell adjacent to the motile ruffled border, organized filament bundles were oriented perpendicular to the cell membrane and terminated in short processes at the bone surface. These filaments were also reactive with HMM. The possible significance of the filaments with respect to the physiology of bone cells is discussed."}
{"id": "PMID:1095604", "title": "Cytoplasmic induction of changes in the ultrastructure of the Acetabularia nucleus and perinuclear cytoplasm.", "content": "The ultrasturcture of the cell nucleus and perinuclear cytoplasm in Acetabularia is quite different in young and old cells. When a nucleus of an old cell was implanted into the cytoplasm of a young cell the nucleus assumed the morphology typical of a young cell within less than 10 days. The cytoplasm of an old cell was able to induce the reverse change in an implanted nucleus from a young cell. This cytoplasmic induction of nuclear ageing appeared to proceed more rapidly than rejuvenation.", "contents": "Cytoplasmic induction of changes in the ultrastructure of the Acetabularia nucleus and perinuclear cytoplasm. The ultrasturcture of the cell nucleus and perinuclear cytoplasm in Acetabularia is quite different in young and old cells. When a nucleus of an old cell was implanted into the cytoplasm of a young cell the nucleus assumed the morphology typical of a young cell within less than 10 days. The cytoplasm of an old cell was able to induce the reverse change in an implanted nucleus from a young cell. This cytoplasmic induction of nuclear ageing appeared to proceed more rapidly than rejuvenation."}
{"id": "PMID:1095605", "title": "Proteins of the newt oocyte nucleus: analysis of the nonhistone proteins from lampbrush chromosomes, nucleoli and nuclear sap.", "content": "An electrophoretic analysis has been carried out on the total protein of lampbrush chromosomes, nucleoli and nuclear sap, obtained from newt oocyte nuclei. In each case, distinctive and heterogeneous banding patterns are observed. The absence of detectable quantities of histones in occyte chromatin is noted. In the case of the lampbrush chromosome preparation, it is concluded that all protein species are derived from the ribonucleoprotein matrix of the lateral loops.", "contents": "Proteins of the newt oocyte nucleus: analysis of the nonhistone proteins from lampbrush chromosomes, nucleoli and nuclear sap. An electrophoretic analysis has been carried out on the total protein of lampbrush chromosomes, nucleoli and nuclear sap, obtained from newt oocyte nuclei. In each case, distinctive and heterogeneous banding patterns are observed. The absence of detectable quantities of histones in occyte chromatin is noted. In the case of the lampbrush chromosome preparation, it is concluded that all protein species are derived from the ribonucleoprotein matrix of the lateral loops."}
{"id": "PMID:1095606", "title": "An ultrastructural study of the spermatozoid of the fern, Marsilea vestita.", "content": "The ultrastructure of the mature spermatozoid of Marsilea vestita was studied after its release from the microspore and prior to its penetration of the egg. The psermatozoid is a pear-shaped cell with a complex spiral structure coiled around the edge in the narrow anterior end. This coil is composed of a large mitochondrion, elongated nucleus with highly condensed chromatin, a ribbon of microtubules, and a dense band of material (flagellated band) into which the flagella are inserted. There are over a hundred flagella protruding from each spermatozoid along the length of the coil. At the anterior tip of the coil is a short multilayered structure. It is not known what maintains the helical shape of the coil. The microtubular ribbon could be involved, but it is also possible that either the flagellated band, the condensed chromatin, or both, are sufficiently rigid to retain their shpaes unaided. When the spermatozoid is first released from the microspore it includes a cytoplasmic vesicle in the posterior region containing plastids, mitochondria, and other organelles. This vesicle is shed, taking the nuclear envelope with it, before the spermatozoid reaches the egg.", "contents": "An ultrastructural study of the spermatozoid of the fern, Marsilea vestita. The ultrastructure of the mature spermatozoid of Marsilea vestita was studied after its release from the microspore and prior to its penetration of the egg. The psermatozoid is a pear-shaped cell with a complex spiral structure coiled around the edge in the narrow anterior end. This coil is composed of a large mitochondrion, elongated nucleus with highly condensed chromatin, a ribbon of microtubules, and a dense band of material (flagellated band) into which the flagella are inserted. There are over a hundred flagella protruding from each spermatozoid along the length of the coil. At the anterior tip of the coil is a short multilayered structure. It is not known what maintains the helical shape of the coil. The microtubular ribbon could be involved, but it is also possible that either the flagellated band, the condensed chromatin, or both, are sufficiently rigid to retain their shpaes unaided. When the spermatozoid is first released from the microspore it includes a cytoplasmic vesicle in the posterior region containing plastids, mitochondria, and other organelles. This vesicle is shed, taking the nuclear envelope with it, before the spermatozoid reaches the egg."}
{"id": "PMID:1095608", "title": "Analysis for carbamate insecticides and metabolites.", "content": "Of the more conventional pesticidal chemicals, the carbamate insecticides pose some unique problems relative to residue analysis. Most of these compounds are unstable under conditions normally used for GLC analysis and require special attention if this technique is to apply to the carbamates. Moreover, the carbamates are commonly metabolized to products which are toxicologically significant and which must be included in any analytical considerations. These and other problems inherent in carbamate residue methodology are discussed in this report along with technique currently utilized or having potential as sound procedures for the analyses of carbamate insecticides.", "contents": "Analysis for carbamate insecticides and metabolites. Of the more conventional pesticidal chemicals, the carbamate insecticides pose some unique problems relative to residue analysis. Most of these compounds are unstable under conditions normally used for GLC analysis and require special attention if this technique is to apply to the carbamates. Moreover, the carbamates are commonly metabolized to products which are toxicologically significant and which must be included in any analytical considerations. These and other problems inherent in carbamate residue methodology are discussed in this report along with technique currently utilized or having potential as sound procedures for the analyses of carbamate insecticides."}
{"id": "PMID:1095610", "title": "Analysis of chlorinated insecticides and congeners.", "content": "A review on the state-of-the-art for analysis of chlorinated insecticides and their congeners is presented. Tables are included that show comparisons among gas chromatographic systems, high speed liquid chromatographic systems and sample preparation methods as well as an overview on sample cleanup.", "contents": "Analysis of chlorinated insecticides and congeners. A review on the state-of-the-art for analysis of chlorinated insecticides and their congeners is presented. Tables are included that show comparisons among gas chromatographic systems, high speed liquid chromatographic systems and sample preparation methods as well as an overview on sample cleanup."}
{"id": "PMID:1095612", "title": "Cyclophosphamide suppression of established cell-mediated immunity.  Quantitative vs. qualitative changes in lymphocyte populations.", "content": "The characteristics of cyclophosphamide-induced suppression of established ccll mediated immunity were studied in guinea pigs previously senstized to tuberculin. Cyclophosphamide treatment for 5 days produced a dose-dependent peripheral lymphoctopenia and disproportionatley  greater neutrophenia which was particularly striking at high doses of 20 mg/kg per day(approximaetly 200 mg/kg-2 per day). Lymphoctes remianing in the circulation of cyclophosphamide treeated aniamls showed a doses-dependent reduction to both in vitro proliferactive and macrophage migration inhibitory factor responses to tuberculin compared to lymphocte responses of controls. Proliferative responses to phytohemaggultinin and concanavalin a were not significatly suppressed. Additional studies showed that cyclophosphamide suppressed the porliferactive and migration inhibitroy factor responses to tuberculin of lymph node and splenic as well as cirulating lymphocte populations. These studies showed that relatively short-term cyclophospamide administration produced immunosuppresion by quantitative as well as qualitative changes in lymphocyte populations. Significant suppresion  of lymphocte function, howerver, was achived only with doses of cyclophoshamide which also produced a severe neutropenia.", "contents": "Cyclophosphamide suppression of established cell-mediated immunity.  Quantitative vs. qualitative changes in lymphocyte populations. The characteristics of cyclophosphamide-induced suppression of established ccll mediated immunity were studied in guinea pigs previously senstized to tuberculin. Cyclophosphamide treatment for 5 days produced a dose-dependent peripheral lymphoctopenia and disproportionatley  greater neutrophenia which was particularly striking at high doses of 20 mg/kg per day(approximaetly 200 mg/kg-2 per day). Lymphoctes remianing in the circulation of cyclophosphamide treeated aniamls showed a doses-dependent reduction to both in vitro proliferactive and macrophage migration inhibitory factor responses to tuberculin compared to lymphocte responses of controls. Proliferative responses to phytohemaggultinin and concanavalin a were not significatly suppressed. Additional studies showed that cyclophosphamide suppressed the porliferactive and migration inhibitroy factor responses to tuberculin of lymph node and splenic as well as cirulating lymphocte populations. These studies showed that relatively short-term cyclophospamide administration produced immunosuppresion by quantitative as well as qualitative changes in lymphocyte populations. Significant suppresion  of lymphocte function, howerver, was achived only with doses of cyclophoshamide which also produced a severe neutropenia."}
{"id": "PMID:1095613", "title": "A comparison of the acute effects of radiation therapy, including or excluding the thymus, on the lymphocyte subpopulations of cancer patients.", "content": "Radiation therapy to either mediastinum or pelvis causes a rapid decrease in circulating lymphocytes of both B and T types and in addition an impairment in the function of the remaining lyphocytes, as measured by their ability to proliferate in response to mitogens.  The acute depression is short-lived.  Substantial recovery is apparent within 3 wk after cessation of therapy; however, most patients show a modest, chronic depression in both numbers and functional capacities of circulating lymphocytes.  T cells are somewhat more sensitive than B cells, but both are affected.  Irradiation of the thymus per se seems to have little influence on the acute changes which occur, as patients receiving pelvic and mediastinal (including thymic) radiotherapy show a similiar degree of lymphopenia and depression of lymphocyte responsiveness.", "contents": "A comparison of the acute effects of radiation therapy, including or excluding the thymus, on the lymphocyte subpopulations of cancer patients. Radiation therapy to either mediastinum or pelvis causes a rapid decrease in circulating lymphocytes of both B and T types and in addition an impairment in the function of the remaining lyphocytes, as measured by their ability to proliferate in response to mitogens.  The acute depression is short-lived.  Substantial recovery is apparent within 3 wk after cessation of therapy; however, most patients show a modest, chronic depression in both numbers and functional capacities of circulating lymphocytes.  T cells are somewhat more sensitive than B cells, but both are affected.  Irradiation of the thymus per se seems to have little influence on the acute changes which occur, as patients receiving pelvic and mediastinal (including thymic) radiotherapy show a similiar degree of lymphopenia and depression of lymphocyte responsiveness."}
{"id": "PMID:1095618", "title": "Pimaricin and mycostatin for retarding cottage cheese spoilage.", "content": "Two antifungal agents, pimaricin and mycostatin, added to Cottage cheese through the wash water at concentrations of 20, 50, or 100 mug/ml of wash water or added through the cheese dressing at 1, 2, or 5 mug/g retarded the growth of Aspergillus niger and Saccharomyces cerevisiae and improved the shelf-life of the cheese. In general, cheese with highest concentration of antifungal agent and stored at lowest temperature had best keeping quality. Pimaricin was slightly more effective than mycostatin in inhibiting fungi; inhibition was greater if the antifungal agents were added to the cheese dressing and the cheese was stored at low temperature; and A. niger was more sensitive to the inhibitors than S. Cerevisiae.", "contents": "Pimaricin and mycostatin for retarding cottage cheese spoilage. Two antifungal agents, pimaricin and mycostatin, added to Cottage cheese through the wash water at concentrations of 20, 50, or 100 mug/ml of wash water or added through the cheese dressing at 1, 2, or 5 mug/g retarded the growth of Aspergillus niger and Saccharomyces cerevisiae and improved the shelf-life of the cheese. In general, cheese with highest concentration of antifungal agent and stored at lowest temperature had best keeping quality. Pimaricin was slightly more effective than mycostatin in inhibiting fungi; inhibition was greater if the antifungal agents were added to the cheese dressing and the cheese was stored at low temperature; and A. niger was more sensitive to the inhibitors than S. Cerevisiae."}
{"id": "PMID:1095626", "title": "Initial mismanagement and delay in diagnosis of oral cancer.", "content": "A study of 779 patients with epidermoid carcinoma of the oral cavity revealed that 115 (14.8%) had had a temporally significant initial mismanagement or delay in diagnosis. The responsibility for this mismanagement was shared by the dentist, the physician, and the patient. Earlier recognition of oral cancer by the physician and dentist, with earlier treatment, is essential for improving the generally poor five-year survival rate of this disease.", "contents": "Initial mismanagement and delay in diagnosis of oral cancer. A study of 779 patients with epidermoid carcinoma of the oral cavity revealed that 115 (14.8%) had had a temporally significant initial mismanagement or delay in diagnosis. The responsibility for this mismanagement was shared by the dentist, the physician, and the patient. Earlier recognition of oral cancer by the physician and dentist, with earlier treatment, is essential for improving the generally poor five-year survival rate of this disease."}
{"id": "PMID:1095622", "title": "Histological response to ivory implanted in rabbit mandibles.", "content": "Ivory rod implants were found to be biologically unacceptable in rabbit mandibles. Fibrous connective-tissue capsules containing numerous multinucleated giant cells formed around the implants. Resorption of the ivory was also apparent. In addition, osteoid deposition and ankylosis at the experimental sites were not evident.", "contents": "Histological response to ivory implanted in rabbit mandibles. Ivory rod implants were found to be biologically unacceptable in rabbit mandibles. Fibrous connective-tissue capsules containing numerous multinucleated giant cells formed around the implants. Resorption of the ivory was also apparent. In addition, osteoid deposition and ankylosis at the experimental sites were not evident."}
{"id": "PMID:1095630", "title": "Evaluation of isobaric oxygenation for the aged with cognitive impairment: pilot study.", "content": "The effect of isobaric oxygenation on psychogeriatric subjects, with mild cognitive impairment was assessed in a single-blind controlled pilot study. Three of the 7 subjects were treated with a mixture of 97% oxygen plus 3% carbon dioxide, and 4 treated with compressed air. The oxygen or air was administered during two 1.5 hr sessions daily for fifteen consecutive days. Use of a miner's type mask ensured a closed system which permits the maximal partial pressure of oxygen. Psychologic and clinical observations did not indicate any advantage for the oxygenation therapy. Minimal side effects, which arose only in the oxygen group, may have involved some interactions between CO2 sensitivity and anxiety. Careful differential diagnosis and the control of motivational factors are of major importance in any study of the effects of oxygenation upon symptoms in psychogeriatric patients.", "contents": "Evaluation of isobaric oxygenation for the aged with cognitive impairment: pilot study. The effect of isobaric oxygenation on psychogeriatric subjects, with mild cognitive impairment was assessed in a single-blind controlled pilot study. Three of the 7 subjects were treated with a mixture of 97% oxygen plus 3% carbon dioxide, and 4 treated with compressed air. The oxygen or air was administered during two 1.5 hr sessions daily for fifteen consecutive days. Use of a miner's type mask ensured a closed system which permits the maximal partial pressure of oxygen. Psychologic and clinical observations did not indicate any advantage for the oxygenation therapy. Minimal side effects, which arose only in the oxygen group, may have involved some interactions between CO2 sensitivity and anxiety. Careful differential diagnosis and the control of motivational factors are of major importance in any study of the effects of oxygenation upon symptoms in psychogeriatric patients."}
{"id": "PMID:1095631", "title": "Electroencephalographic and behavioral changes associated with papaverine administration in healthy geriatric subjects.", "content": "A double-blind cross-over procedure was used to evaluate the effects of papaverine hydrochloride on the electroencephalogram (EEG), cognition, mood and psychologic test performance of 10 healthy geriatric volunteers. During the two-week period, each subject was tested four times: before and after receiving papaverine and before and after receiving placebo. Period analysis revealed that a 300-mg dose of papaverine (Pavabid Plateau Caps) increased EEG alpha activity (8-13 cycles per second) and decreased the beta2 range (27-40 cps). Baseline values for six mood factors, short-term memory, complex problem solving, and attention were not altered significantly by either drug or placebo. Results of the Subject Paced Digit Symbol Substitution Test indicated that papaverine may produce some improvement in simple cognitive functioning. No adverse effects were associated with the use of papaverine.", "contents": "Electroencephalographic and behavioral changes associated with papaverine administration in healthy geriatric subjects. A double-blind cross-over procedure was used to evaluate the effects of papaverine hydrochloride on the electroencephalogram (EEG), cognition, mood and psychologic test performance of 10 healthy geriatric volunteers. During the two-week period, each subject was tested four times: before and after receiving papaverine and before and after receiving placebo. Period analysis revealed that a 300-mg dose of papaverine (Pavabid Plateau Caps) increased EEG alpha activity (8-13 cycles per second) and decreased the beta2 range (27-40 cps). Baseline values for six mood factors, short-term memory, complex problem solving, and attention were not altered significantly by either drug or placebo. Results of the Subject Paced Digit Symbol Substitution Test indicated that papaverine may produce some improvement in simple cognitive functioning. No adverse effects were associated with the use of papaverine."}
{"id": "PMID:1095632", "title": "Medical experimentation in the elderly.", "content": "Participation in human experimental research constitutes a major problem for the geriatric subject. Because there is a high incidence of noncontagious disease in the elderly, they are the group most useful for the study of new therapeutic agents or procedures. However, normal aging processes, often coupled with disease of the central nervous system, render elderly persons less able to comprehend the nature and risks of such studies. These factors permit easy exploitation of geriatric subjects in medical experimentation, with possible exposure to a significant risk of serious drug reactions and unnecessary hospitalization. Recent federal regulations have given \"special protections\" to children, prisoners, and the mentally infirm in experimental research, to guard against abuse of their human rights. A basic requirement is that informed consent be carefully obtained and documented. Such \"special protections\" should now be extended to geriatric subjects so that there will be no further exploitation in the course of valid clinical research.", "contents": "Medical experimentation in the elderly. Participation in human experimental research constitutes a major problem for the geriatric subject. Because there is a high incidence of noncontagious disease in the elderly, they are the group most useful for the study of new therapeutic agents or procedures. However, normal aging processes, often coupled with disease of the central nervous system, render elderly persons less able to comprehend the nature and risks of such studies. These factors permit easy exploitation of geriatric subjects in medical experimentation, with possible exposure to a significant risk of serious drug reactions and unnecessary hospitalization. Recent federal regulations have given \"special protections\" to children, prisoners, and the mentally infirm in experimental research, to guard against abuse of their human rights. A basic requirement is that informed consent be carefully obtained and documented. Such \"special protections\" should now be extended to geriatric subjects so that there will be no further exploitation in the course of valid clinical research."}
{"id": "PMID:1095621", "title": "Chemical composition of the washed cells of Streptococcus sanguis (804) and Streptococcus mutans (B-14).", "content": "Washed cells of Streptococcus saguis (804) and Streptococcus mutans (B14) were analyzed for water, ash, protein, carbohydrate, lipid, and nucleic acid content. Lipids were analyzed by gas chromatography. Ash was subjected to elemental analysis. These data were compared with the published values for the composition of dental plaque.", "contents": "Chemical composition of the washed cells of Streptococcus sanguis (804) and Streptococcus mutans (B-14). Washed cells of Streptococcus saguis (804) and Streptococcus mutans (B14) were analyzed for water, ash, protein, carbohydrate, lipid, and nucleic acid content. Lipids were analyzed by gas chromatography. Ash was subjected to elemental analysis. These data were compared with the published values for the composition of dental plaque."}
{"id": "PMID:1095649", "title": "Deoxyribonucleic acid fiber autoradiography as a technique for studying the replication of the mammalian chromosome.", "content": "Deoxyribonucleic acid (DNA) fiber autoradiography is a technique that allows analysis of replication events on mammalian chromosomal DNA. 3H-thymidine is used to pulse-label DNA, which is subsequently released from lysed cells and extended linearly over a glass slide. Autoradiograms are produced by exposing the labeled DNA to light-sensitive emulsion. The effects of inhibition of protein synthesis on DNA replication were examined using this technique. Analysis of the autoradiographic patterns has shown that inhibition of protein synthesis with either cycloheximide or puromycin retards the rate of replication fork progression. In addition, these antibiotics after the normal pattern of multifocal initiation on replication units. The spatial interval between active initiation sites is decreased, there is a decay in synchrony of initiation events on subchromosomal clusters of replication units, the decay in synchrony of initiation events on subchromosomal clusters of replication units, the frequency of initiation is inhibited and the frequency of units showing the normal bidirectional mode of replication is decreased. The retarded fork progression and shorter initiation intervals may result either from the continued operation of a subset of replication units resistant to the inhibition of protein synthesis, or be manifestations of the inhibition of protein synthesis on all active sites. The other alterations, decreased synchrony and frequency of initiation and increased unidirectional replication probably occur on all active units when protein synthesis is inhibited.", "contents": "Deoxyribonucleic acid fiber autoradiography as a technique for studying the replication of the mammalian chromosome. Deoxyribonucleic acid (DNA) fiber autoradiography is a technique that allows analysis of replication events on mammalian chromosomal DNA. 3H-thymidine is used to pulse-label DNA, which is subsequently released from lysed cells and extended linearly over a glass slide. Autoradiograms are produced by exposing the labeled DNA to light-sensitive emulsion. The effects of inhibition of protein synthesis on DNA replication were examined using this technique. Analysis of the autoradiographic patterns has shown that inhibition of protein synthesis with either cycloheximide or puromycin retards the rate of replication fork progression. In addition, these antibiotics after the normal pattern of multifocal initiation on replication units. The spatial interval between active initiation sites is decreased, there is a decay in synchrony of initiation events on subchromosomal clusters of replication units, the decay in synchrony of initiation events on subchromosomal clusters of replication units, the frequency of initiation is inhibited and the frequency of units showing the normal bidirectional mode of replication is decreased. The retarded fork progression and shorter initiation intervals may result either from the continued operation of a subset of replication units resistant to the inhibition of protein synthesis, or be manifestations of the inhibition of protein synthesis on all active sites. The other alterations, decreased synchrony and frequency of initiation and increased unidirectional replication probably occur on all active units when protein synthesis is inhibited."}
{"id": "PMID:1095650", "title": "Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence.", "content": "A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid with an asymmetric distribution of thymine residues between polynucleotide chains and (c) the detection of late replicating regions in metaphase chromosomes. The suppression of 33258 Hoechst fluorescence by 5-bromodeoxyuridine incorporated biosynthetically into interphase nuclei is demonstrated both in fixed cytologic preparations and in unfixed cultured cells. Many of the cytologic observations described might form a basis for future biochemical studies.", "contents": "Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid with an asymmetric distribution of thymine residues between polynucleotide chains and (c) the detection of late replicating regions in metaphase chromosomes. The suppression of 33258 Hoechst fluorescence by 5-bromodeoxyuridine incorporated biosynthetically into interphase nuclei is demonstrated both in fixed cytologic preparations and in unfixed cultured cells. Many of the cytologic observations described might form a basis for future biochemical studies."}
{"id": "PMID:1095654", "title": "The macrophage electrophoretic mobility test (MEM) some technical considerations.", "content": "The following parameters of the cytopherometric test system have been studied. 1. Temperature is less important for the production of macrophage slowing factor (MSF) by lymphocyte-antigen interaction than it is for the activity of MSF on macrophages. 2. Lymphocyte number titration curves allow discrimination between 'weak' and 'strong' antigen responses. 3. Lymphocyte-antigen reaction reaches a plateau after 30 min incubation, whilst maximum MSF-macrophage reaction requires 60 min. 4. Interaction of MSF with macrophages is sensitive to cycloheximide. 5. Irradiation of peritoneal exudate cells (200 rad) is essential in order to obtain a maximal result in the MEM test. In the 'split MEM test, 200 rad to the indicator peritoneal exudate cells produces a greater result, i.e. it in some way amplifies the macrophage response to MSF. 6. Excess of peritoneal exudate cells, especially when non-irradiated, suppresses the test result. This is not due to consumption of MSF produced. Probably macrophages rather than lymphocytes are responsible for the suppression.", "contents": "The macrophage electrophoretic mobility test (MEM) some technical considerations. The following parameters of the cytopherometric test system have been studied. 1. Temperature is less important for the production of macrophage slowing factor (MSF) by lymphocyte-antigen interaction than it is for the activity of MSF on macrophages. 2. Lymphocyte number titration curves allow discrimination between 'weak' and 'strong' antigen responses. 3. Lymphocyte-antigen reaction reaches a plateau after 30 min incubation, whilst maximum MSF-macrophage reaction requires 60 min. 4. Interaction of MSF with macrophages is sensitive to cycloheximide. 5. Irradiation of peritoneal exudate cells (200 rad) is essential in order to obtain a maximal result in the MEM test. In the 'split MEM test, 200 rad to the indicator peritoneal exudate cells produces a greater result, i.e. it in some way amplifies the macrophage response to MSF. 6. Excess of peritoneal exudate cells, especially when non-irradiated, suppresses the test result. This is not due to consumption of MSF produced. Probably macrophages rather than lymphocytes are responsible for the suppression."}
{"id": "PMID:1095655", "title": "A method for the selection of pig lymphocytes bearing surface immunoglobulin.", "content": "Coupling of IgG anti-immunoglobulin to polystyrene bottles with carbodiimide has been used to bind selectively pig lymphocytes previously treated with anti-immunoglobulin or anti-Ig class-specific fluorescent conjugates. Subsequent recovery of the bound cells produced populations with approximately 80% of cells stained for surface Ig and 90% viability.", "contents": "A method for the selection of pig lymphocytes bearing surface immunoglobulin. Coupling of IgG anti-immunoglobulin to polystyrene bottles with carbodiimide has been used to bind selectively pig lymphocytes previously treated with anti-immunoglobulin or anti-Ig class-specific fluorescent conjugates. Subsequent recovery of the bound cells produced populations with approximately 80% of cells stained for surface Ig and 90% viability."}
{"id": "PMID:1095656", "title": "Quick and easy production of monolayer plaque assay slides.", "content": "A fast and easy method for the production of monolayer plaque-assay slides, making use of double adhesive tape, is described.", "contents": "Quick and easy production of monolayer plaque assay slides. A fast and easy method for the production of monolayer plaque-assay slides, making use of double adhesive tape, is described."}
{"id": "PMID:1095660", "title": "Long-term maintenance of psoriatic human skin on congenitally athymic (nude) mice.", "content": "Transplantation of involved psoriatic and nonpsoriatic human skin onto congenitally athymic (nude) mice has been performed successfully. Although biopsies at selected intervals demonstrate that the excess glycogen deposition normally seen in psoriasis is no longer consistently present, the psoriatic grafts did retain the usual characteristic histologic differences throughout the life of the animal, up to 11 weeks. This grafting procedure potentially represents a useful method whereby the study of psoriasis can be made in a nonhuman, living system.", "contents": "Long-term maintenance of psoriatic human skin on congenitally athymic (nude) mice. Transplantation of involved psoriatic and nonpsoriatic human skin onto congenitally athymic (nude) mice has been performed successfully. Although biopsies at selected intervals demonstrate that the excess glycogen deposition normally seen in psoriasis is no longer consistently present, the psoriatic grafts did retain the usual characteristic histologic differences throughout the life of the animal, up to 11 weeks. This grafting procedure potentially represents a useful method whereby the study of psoriasis can be made in a nonhuman, living system."}
{"id": "PMID:1095661", "title": "Mitochondria and nuclei of pig and human epidermis: isolation and lipid composition.", "content": "Pig and human epidermis were mechanically disrupted with a Polytron homogenizer and mitochondria and nuclei were isolated from homogenates in buffered sucrose and 2.5% citric acid solution, respectively, by differential and density-gradient centrifugation. Examination of the mitochondrial preparations by electron microscopy and the assay of several marker enzymes indicated that they consisted mostly of mitochondria with some microsomal contamination. The nuclear preparations were substantially free from contaminants as judged by particle counts with the aid of phase and electron microscopy. The lipid compositions of the mitochondria from both species were characterized by a high concentration of cardiolipin and low concentration of cholesterol and sphingomyelin and an absence of glycosphingolipids as compared with the lipid composition of the whole epidermal cell. The lipid compositions of the nuclei were characterized by a high proportion of phosphatidyl choline and an absence of glycosphingolipids. The neutral lipids accounted for an unusually high proportion of the total lipids especially in human epidermal nuclei. Although the technical difficulties in isolating subcellular organelles from mammalian epidermis limit the yield and quality of the mitochondria, these preparations as well as those of nuclei are superior to preparations reported previously and are pure enough for a valid comparison with other membrane systems of the epidermal cell.", "contents": "Mitochondria and nuclei of pig and human epidermis: isolation and lipid composition. Pig and human epidermis were mechanically disrupted with a Polytron homogenizer and mitochondria and nuclei were isolated from homogenates in buffered sucrose and 2.5% citric acid solution, respectively, by differential and density-gradient centrifugation. Examination of the mitochondrial preparations by electron microscopy and the assay of several marker enzymes indicated that they consisted mostly of mitochondria with some microsomal contamination. The nuclear preparations were substantially free from contaminants as judged by particle counts with the aid of phase and electron microscopy. The lipid compositions of the mitochondria from both species were characterized by a high concentration of cardiolipin and low concentration of cholesterol and sphingomyelin and an absence of glycosphingolipids as compared with the lipid composition of the whole epidermal cell. The lipid compositions of the nuclei were characterized by a high proportion of phosphatidyl choline and an absence of glycosphingolipids. The neutral lipids accounted for an unusually high proportion of the total lipids especially in human epidermal nuclei. Although the technical difficulties in isolating subcellular organelles from mammalian epidermis limit the yield and quality of the mitochondria, these preparations as well as those of nuclei are superior to preparations reported previously and are pure enough for a valid comparison with other membrane systems of the epidermal cell."}
{"id": "PMID:1095668", "title": "The application of trace element analysis by X-ray fluorescence to human blood serum.", "content": "The need for a simple, yet fast and accurate method for trace element analysis of human tissues is remarded on. Such a method is the use of x-ray fluorescence in conjunction with one of the new generation of solid-state x-ray detectors. The method is described, together with an example of its application-apilot study of forty-two blood serum samples supplied by the Manitoba Cancer Research Institute.", "contents": "The application of trace element analysis by X-ray fluorescence to human blood serum. The need for a simple, yet fast and accurate method for trace element analysis of human tissues is remarded on. Such a method is the use of x-ray fluorescence in conjunction with one of the new generation of solid-state x-ray detectors. The method is described, together with an example of its application-apilot study of forty-two blood serum samples supplied by the Manitoba Cancer Research Institute."}
{"id": "PMID:1095669", "title": "Measurement of redox potential changes in anaerobic culture media caused by addition of blood.", "content": "A method has been described whereby the redox potential of unsealed and manipulated samples of solution can be determined. This method includes treating the platinum electrode with strong cleaning solution, adjusting the electrode with a culture of Proteus vulgaris in tryptic soy broth, and doing all manipulations inside a glove box that is continuously flushed with oxygen-free gas. This study has shown that prereduced media are much more reduced than media which have been dispensed only under a partial vacuum. It also has shown that addition of blood to prereduced medium does not eliminate the strongly reducing condition of the medium although there is a slight rise in redox potential.", "contents": "Measurement of redox potential changes in anaerobic culture media caused by addition of blood. A method has been described whereby the redox potential of unsealed and manipulated samples of solution can be determined. This method includes treating the platinum electrode with strong cleaning solution, adjusting the electrode with a culture of Proteus vulgaris in tryptic soy broth, and doing all manipulations inside a glove box that is continuously flushed with oxygen-free gas. This study has shown that prereduced media are much more reduced than media which have been dispensed only under a partial vacuum. It also has shown that addition of blood to prereduced medium does not eliminate the strongly reducing condition of the medium although there is a slight rise in redox potential."}
{"id": "PMID:1095670", "title": "Cellular immunity to intrinsic factor in pernicious anemia.", "content": "Cellular immunity to hog intrinsic factor was detected by a modified agarose-leukocyte migration test in 18 patients with pernicious amemia. Lymphocytes from 17 out of 18 patients with pernicious anemia gave positive responses  to a concentrate of hog intrinsic factor; the intrinsic factor present in 1 mg. of this concentrate bound 128 ng. of vatamin B12. Six patients with atrophic gastritis, 7 with regional enteritis, and 9 out of 10 healthy adults did not respond to this preparation. No correlation existed between the presence of serum autoantibodies to intrinsic factor and in vitro lymphocyte responsiveness to intrinsic factor. The results demonstrate that cellular immunity to intrinsic factor concentrates is present in the majority of patients with pernicious anemia.", "contents": "Cellular immunity to intrinsic factor in pernicious anemia. Cellular immunity to hog intrinsic factor was detected by a modified agarose-leukocyte migration test in 18 patients with pernicious amemia. Lymphocytes from 17 out of 18 patients with pernicious anemia gave positive responses  to a concentrate of hog intrinsic factor; the intrinsic factor present in 1 mg. of this concentrate bound 128 ng. of vatamin B12. Six patients with atrophic gastritis, 7 with regional enteritis, and 9 out of 10 healthy adults did not respond to this preparation. No correlation existed between the presence of serum autoantibodies to intrinsic factor and in vitro lymphocyte responsiveness to intrinsic factor. The results demonstrate that cellular immunity to intrinsic factor concentrates is present in the majority of patients with pernicious anemia."}
{"id": "PMID:1095671", "title": "Results of 310 cases of rapid maxillary expansion selected for medical reasons.", "content": "1. Rapid Maxillary Expansion is a comparatively simple, conservative method of treating impaired nasal respiration. 2. It can be used from 4 years to 25 or 30 years of age, but the younger the patient the better the long term results. 3. The medical indications are a combination of poor nasal airway, septal deformity, recurrent ear or nasal infection, allergic rhinitis and asthma, and prior to septoplasty. 4. The dental indications are mainly unilateral or bilateral cross bite, class III (prognathoid) malocclusion, maxillary compression, and cleft palate. 5. The dental contra-indications are normal occlusion in the permanent dentition and to a lesser degree severe class II occlusion and micromandible. 6. In selected cases, it produces a change of over 80 percent from mouth to nose breathing. In this series of 310 consecutive cases 87 percent were greatly improved, 9 percent had a fair result and 4 percent a poor result. 7. It also gives considerable improvement in colds and respiratory infection, nasal allergy and many cases of asthma. The improvement in health and confidence and concentration in over 30 percent of cases is particularly pleasing. 8. The rationale of Rapid Maxillary Expansion can be explained by the maxillary moulding theory of production of septal and facial deformities.", "contents": "Results of 310 cases of rapid maxillary expansion selected for medical reasons. 1. Rapid Maxillary Expansion is a comparatively simple, conservative method of treating impaired nasal respiration. 2. It can be used from 4 years to 25 or 30 years of age, but the younger the patient the better the long term results. 3. The medical indications are a combination of poor nasal airway, septal deformity, recurrent ear or nasal infection, allergic rhinitis and asthma, and prior to septoplasty. 4. The dental indications are mainly unilateral or bilateral cross bite, class III (prognathoid) malocclusion, maxillary compression, and cleft palate. 5. The dental contra-indications are normal occlusion in the permanent dentition and to a lesser degree severe class II occlusion and micromandible. 6. In selected cases, it produces a change of over 80 percent from mouth to nose breathing. In this series of 310 consecutive cases 87 percent were greatly improved, 9 percent had a fair result and 4 percent a poor result. 7. It also gives considerable improvement in colds and respiratory infection, nasal allergy and many cases of asthma. The improvement in health and confidence and concentration in over 30 percent of cases is particularly pleasing. 8. The rationale of Rapid Maxillary Expansion can be explained by the maxillary moulding theory of production of septal and facial deformities."}
{"id": "PMID:1095672", "title": "Primary amyloidosis of the trachea.", "content": "A case of primary amyloidosis of the trachea in a 40-year-old female is described. It appeared clinically as a tumour-like lesion in the trachea. The specificity of amyloid deposits was confirmed by a positive reaction to Congo red stained sections viewed under polarised light and Thioflavine--T stained section showing characteristic fluorescence. Excision of the lesion with a split thickness graft was the mode of treatment.", "contents": "Primary amyloidosis of the trachea. A case of primary amyloidosis of the trachea in a 40-year-old female is described. It appeared clinically as a tumour-like lesion in the trachea. The specificity of amyloid deposits was confirmed by a positive reaction to Congo red stained sections viewed under polarised light and Thioflavine--T stained section showing characteristic fluorescence. Excision of the lesion with a split thickness graft was the mode of treatment."}
{"id": "PMID:1095677", "title": "An alternate method for calculating an odds ratio.", "content": "Methodological problems involved in the use of the standard Woolf-Haldane analysis of epidemiological retrospective studies are examined and an alternate method of analysis is proposed. This alternate method involves a population constructed to match the cases in numerical size and to match the controls in proportion of exposures. This method allows for finer subclassification of the data and provides a meaningful summary estimate of the relative risk. The proposed method is contrasted with the Woolf-Haldane method in the analysis of the relative risk of exposure to sick pet bird versus no pet bird for adult leukemia cases versus controls. Data is from the Tri-State Leukemia Survey. Mathematical considerations involved are contained in the appendices.", "contents": "An alternate method for calculating an odds ratio. Methodological problems involved in the use of the standard Woolf-Haldane analysis of epidemiological retrospective studies are examined and an alternate method of analysis is proposed. This alternate method involves a population constructed to match the cases in numerical size and to match the controls in proportion of exposures. This method allows for finer subclassification of the data and provides a meaningful summary estimate of the relative risk. The proposed method is contrasted with the Woolf-Haldane method in the analysis of the relative risk of exposure to sick pet bird versus no pet bird for adult leukemia cases versus controls. Data is from the Tri-State Leukemia Survey. Mathematical considerations involved are contained in the appendices."}
{"id": "PMID:1095678", "title": "The impedance machine: a new bedside coagulation recording device.", "content": "A new continuously recording bedside coagulation screening device is described. The instrument, called an empedance machine, functions by measuring the changing mechanical impedance exerted on a minutely vibrating probe by the progressing fibrin formation of the specimen to be analyzed. The impedance machine works with 0.04-0.4 ml both of whole blood or plasma. During the recordings, the clotting process can be observed in the specimens which are transilluminated. The rugged machine is extremely simple to handle. The two parts which come in contact with blood are disposable. Various examples (recordings) for clinical and experimental applicaton are given and discussed.", "contents": "The impedance machine: a new bedside coagulation recording device. A new continuously recording bedside coagulation screening device is described. The instrument, called an empedance machine, functions by measuring the changing mechanical impedance exerted on a minutely vibrating probe by the progressing fibrin formation of the specimen to be analyzed. The impedance machine works with 0.04-0.4 ml both of whole blood or plasma. During the recordings, the clotting process can be observed in the specimens which are transilluminated. The rugged machine is extremely simple to handle. The two parts which come in contact with blood are disposable. Various examples (recordings) for clinical and experimental applicaton are given and discussed."}
{"id": "PMID:1095680", "title": "Termination of aquired and natural immunological tolerance with specific complexes.", "content": "It was possible to terminate the induced unresponsive state to bovine serum albumin (BSA) and the natural unresponsive state to autologous thyroglobulin in rabbits (RTg) by immunization with complexes composed of heterologous cross-reacting antibody and the tolerated antigens. The unresponsive state was terminated in rabbits made unresponsive by neonatal injections of BSA and then 3 mo later injected with complexes composed of BSA and guinea pig antihuman serum albumin. This termination was manifested by the presence of anti-BSA plaque-forming cells. Similarly, the natural unresponsive state was terminated in adult rabbits injected with complexes between RTg and guinea pig antibovine thyroglobulin (BTg) in that thyroid lesions and circulating anti-RTg were produced. The results can be best explained by the presence of unresponsive T cells and competent B cells, where the guinea pig gamma globulin (antibody) activates T cells specific for the guinea pig gamma globulin portion of the complexes and thus permits stimulation of B cells competent to the exposed determinants of the tolerated (BSA or RTg) portion of the complexes. The detailed mechanism for the activation of B cells in tolerant animals is discussed.", "contents": "Termination of aquired and natural immunological tolerance with specific complexes. It was possible to terminate the induced unresponsive state to bovine serum albumin (BSA) and the natural unresponsive state to autologous thyroglobulin in rabbits (RTg) by immunization with complexes composed of heterologous cross-reacting antibody and the tolerated antigens. The unresponsive state was terminated in rabbits made unresponsive by neonatal injections of BSA and then 3 mo later injected with complexes composed of BSA and guinea pig antihuman serum albumin. This termination was manifested by the presence of anti-BSA plaque-forming cells. Similarly, the natural unresponsive state was terminated in adult rabbits injected with complexes between RTg and guinea pig antibovine thyroglobulin (BTg) in that thyroid lesions and circulating anti-RTg were produced. The results can be best explained by the presence of unresponsive T cells and competent B cells, where the guinea pig gamma globulin (antibody) activates T cells specific for the guinea pig gamma globulin portion of the complexes and thus permits stimulation of B cells competent to the exposed determinants of the tolerated (BSA or RTg) portion of the complexes. The detailed mechanism for the activation of B cells in tolerant animals is discussed."}
{"id": "PMID:1095681", "title": "The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen.", "content": "The lipoprotein of the outer membrane of Escherichia coli is a B-cell mitogen in mice.  Polyclonal activation of B lymphocytes was measured by an increase in thymidine uptake, by the development of plaque-forming cells against densely coupled trinitrophenylated sheep red cells, and by selectively increased rates of synthesis and secretion of leucine-labeled IgM. Murein-free and muropeptides-containing lipoprotein are effective in B-cell activation, while free murein is inactive.  Removal of ester-linked fatty acids from the amino-terminal end of the lipoprotein by alkaline hydrolysis abolishes the mitogenicity of the lipoprotein. B lymphocytes from high responder (C3H/Tif and BALB/c nu/nu) or from low responder (C3H/HeJ) mice to the mitogen lipopolysaccharide (LPS) both respond well to the lipoprotein.  Anti-immunoglobulin antibodies inhibit the mitogenic stimulation of B cells by lipoprotein.  A complex of structures including the Ig-receptor molecules, the LPS receptor, and the lipoprotein receptor appear involved in the regulation of mitogenic stimulation of B cells to proliferation and differentiation to IgM-secreting cells.", "contents": "The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen. The lipoprotein of the outer membrane of Escherichia coli is a B-cell mitogen in mice.  Polyclonal activation of B lymphocytes was measured by an increase in thymidine uptake, by the development of plaque-forming cells against densely coupled trinitrophenylated sheep red cells, and by selectively increased rates of synthesis and secretion of leucine-labeled IgM. Murein-free and muropeptides-containing lipoprotein are effective in B-cell activation, while free murein is inactive.  Removal of ester-linked fatty acids from the amino-terminal end of the lipoprotein by alkaline hydrolysis abolishes the mitogenicity of the lipoprotein. B lymphocytes from high responder (C3H/Tif and BALB/c nu/nu) or from low responder (C3H/HeJ) mice to the mitogen lipopolysaccharide (LPS) both respond well to the lipoprotein.  Anti-immunoglobulin antibodies inhibit the mitogenic stimulation of B cells by lipoprotein.  A complex of structures including the Ig-receptor molecules, the LPS receptor, and the lipoprotein receptor appear involved in the regulation of mitogenic stimulation of B cells to proliferation and differentiation to IgM-secreting cells."}
{"id": "PMID:1095682", "title": "Studies on cell differentiation: inducing capacity of sulfhydryl-containing amino acids on post-nodal pieces of chick blastoderms.", "content": "The inducing capacity of sulfhydryl (SH)-containing amino acids (cysteine and glutathione) on post-nodal pieces (PNPs) of stage 4 chick blastoderms was investigated. PNPs were treated for different lengths of time with chick Ringer's solution (control group) or chick Ringer's solution containing cysteine or glutathione, followed by culturing for 2-10 days on Spratt-Haas agar medium or on the chorioallantoic membrane of 8-day chick embryos. Control PNPs rarely showed differentiation, but those treated with the amino acids for six hours or longer developed structures such as neural tissue, notochord, somite mesoderm, and nephric tubules. The pulsatile tissue was only seen in the PNPs cultured for four days or longer. Two-four hours of treatment was too short to provoke induction in a statistically significant number of PNPs. The highest frequency of induction was noted in those pretreated with glutathione (8 mug/ml) for eight hours, followed by culturing for four days. The magnitude of the inducing capacity and toxicity of the amino acids were concentration dependent: a deleterious effect was observed at 14 mug/ml; the highest frequency of induction occurred at 8 mug/ml, but the frequency decreased as the concentration decreased; at 2 mug/ml all PNPs remained viable, but only a few (9-14%) showed differentiation. The inducing capacity of the amino acids was counteracted by equimolar concentrations of rho-chloromercuribenzoic acid or omega-chloroacetophenone. The effects of glutathione (8 mug/ml) differed from those of Hensen's node grafts in that the former caused sublethal cytolysis and inhibited H-3-uridine uptake in competent ectodermal cells during the first 18 hours of cultivation.", "contents": "Studies on cell differentiation: inducing capacity of sulfhydryl-containing amino acids on post-nodal pieces of chick blastoderms. The inducing capacity of sulfhydryl (SH)-containing amino acids (cysteine and glutathione) on post-nodal pieces (PNPs) of stage 4 chick blastoderms was investigated. PNPs were treated for different lengths of time with chick Ringer's solution (control group) or chick Ringer's solution containing cysteine or glutathione, followed by culturing for 2-10 days on Spratt-Haas agar medium or on the chorioallantoic membrane of 8-day chick embryos. Control PNPs rarely showed differentiation, but those treated with the amino acids for six hours or longer developed structures such as neural tissue, notochord, somite mesoderm, and nephric tubules. The pulsatile tissue was only seen in the PNPs cultured for four days or longer. Two-four hours of treatment was too short to provoke induction in a statistically significant number of PNPs. The highest frequency of induction was noted in those pretreated with glutathione (8 mug/ml) for eight hours, followed by culturing for four days. The magnitude of the inducing capacity and toxicity of the amino acids were concentration dependent: a deleterious effect was observed at 14 mug/ml; the highest frequency of induction occurred at 8 mug/ml, but the frequency decreased as the concentration decreased; at 2 mug/ml all PNPs remained viable, but only a few (9-14%) showed differentiation. The inducing capacity of the amino acids was counteracted by equimolar concentrations of rho-chloromercuribenzoic acid or omega-chloroacetophenone. The effects of glutathione (8 mug/ml) differed from those of Hensen's node grafts in that the former caused sublethal cytolysis and inhibited H-3-uridine uptake in competent ectodermal cells during the first 18 hours of cultivation."}
{"id": "PMID:1095683", "title": "Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli.", "content": "A derepressed R factor, RY2drd2, was transferred at a frequency of 5x10minus 3 between two strains of Yersinia enterocolitica mated on a membrane. Under the same conditions transfer of this R factor from Escherichia coli to Y. enterocolitica was observed at a frequency of only 7-7x10minus 6. This frequency was greatly increased when the recipient strain was heat-treated before mating. Heat exposure for optimum fertility was 50 to 52 degrees C for a period of 2 to 3 min. Mutants of Y. enterocolitica were isolated which were infected by RY2drd2 from E. coli or from Y. enterocolitica at the same frequency. These observations strongly suggest that a DNA restriction and modification system in Y. enterocolitica causes its low recipient ability for plasmids from other species.", "contents": "Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli. A derepressed R factor, RY2drd2, was transferred at a frequency of 5x10minus 3 between two strains of Yersinia enterocolitica mated on a membrane. Under the same conditions transfer of this R factor from Escherichia coli to Y. enterocolitica was observed at a frequency of only 7-7x10minus 6. This frequency was greatly increased when the recipient strain was heat-treated before mating. Heat exposure for optimum fertility was 50 to 52 degrees C for a period of 2 to 3 min. Mutants of Y. enterocolitica were isolated which were infected by RY2drd2 from E. coli or from Y. enterocolitica at the same frequency. These observations strongly suggest that a DNA restriction and modification system in Y. enterocolitica causes its low recipient ability for plasmids from other species."}
{"id": "PMID:1095684", "title": "R factors from Proteus mirabilis and P. vulgaris.", "content": "Eighty-nine R factors were transmitted by conjugation to Escherichia coli K12 from isolates of Proteus hauseri (P. mirabilis  plus P vulgaris). More than half were non-selftranmissible. The remainder included plasmids assigned to the previously defined groups FII,A-C complex, J, N and P, as well as some not belonging to any knwon compatibility groups. R factors from strains isolated in India, Thailand and Japan carried plasmids whose inheritance was extremely unstable in E. coli K12. All belonged to a new compatibility group, V.", "contents": "R factors from Proteus mirabilis and P. vulgaris. Eighty-nine R factors were transmitted by conjugation to Escherichia coli K12 from isolates of Proteus hauseri (P. mirabilis  plus P vulgaris). More than half were non-selftranmissible. The remainder included plasmids assigned to the previously defined groups FII,A-C complex, J, N and P, as well as some not belonging to any knwon compatibility groups. R factors from strains isolated in India, Thailand and Japan carried plasmids whose inheritance was extremely unstable in E. coli K12. All belonged to a new compatibility group, V."}
{"id": "PMID:1095685", "title": "Construction and phenotypes of double sporulation deficient mutants in Streptomyces coelicolor A3(2).", "content": "In whiA, B, G and H mutants of Streptomyces coelicolor A3(2), aerial hyphae develop but sporulation speta are not formed. Septa are produced by whiI mutants but are spaced abnormally far apart. Mutants in each locus have a distinctive aerial mycelium morphology, except for whiA and B mutants which are closely similar. Seven strains were made with pairwise combinations of whiA and B mutations with whiG, H and I mutations and with each other. The genotypes of these strains were confirmed by suitable crosses and their aerial mycelium morphology examined. An indirect procedure was used to determine the aerial mycelium morphology of whiGH, GI and HI double mutants. The double mutants always closely resembled one of the single mutant parent strains in morphology and a consistent scheme of epistasis was obtained--whiG being epistatic to whiH, A, B and I; whiH to whiA, B and I; and whiA or B to whiI. These results point to the absence of any complex interactions between gene products, which might have been revealed by the occurrence of novel phenotypes in double mutants or by inconsistencies in the epistasis scheme.", "contents": "Construction and phenotypes of double sporulation deficient mutants in Streptomyces coelicolor A3(2). In whiA, B, G and H mutants of Streptomyces coelicolor A3(2), aerial hyphae develop but sporulation speta are not formed. Septa are produced by whiI mutants but are spaced abnormally far apart. Mutants in each locus have a distinctive aerial mycelium morphology, except for whiA and B mutants which are closely similar. Seven strains were made with pairwise combinations of whiA and B mutations with whiG, H and I mutations and with each other. The genotypes of these strains were confirmed by suitable crosses and their aerial mycelium morphology examined. An indirect procedure was used to determine the aerial mycelium morphology of whiGH, GI and HI double mutants. The double mutants always closely resembled one of the single mutant parent strains in morphology and a consistent scheme of epistasis was obtained--whiG being epistatic to whiH, A, B and I; whiH to whiA, B and I; and whiA or B to whiI. These results point to the absence of any complex interactions between gene products, which might have been revealed by the occurrence of novel phenotypes in double mutants or by inconsistencies in the epistasis scheme."}
{"id": "PMID:1095686", "title": "Polyene sensitivity during germination of conidia of Aspergillus fumigatus.", "content": "A system for the rapid and relatively synchromous germination of conidia from a clinical isolate of Aspergillus fumigatus is described. The polyene-mediated release of K plus from germinating conidia has been determined. Ungerminated conidia were insensitive to amphotericin B methyl ester (AME) at concentrations greater than 50 mug/ml, but rapidly became sensitive to 1 to 2 mug AME/ml during the intitial stages of germination. These findings have been correlated with minimum inhibitory concentration values obtained in studies of conidial germination and hyphal outgrowth using a variety of growth tests.", "contents": "Polyene sensitivity during germination of conidia of Aspergillus fumigatus. A system for the rapid and relatively synchromous germination of conidia from a clinical isolate of Aspergillus fumigatus is described. The polyene-mediated release of K plus from germinating conidia has been determined. Ungerminated conidia were insensitive to amphotericin B methyl ester (AME) at concentrations greater than 50 mug/ml, but rapidly became sensitive to 1 to 2 mug AME/ml during the intitial stages of germination. These findings have been correlated with minimum inhibitory concentration values obtained in studies of conidial germination and hyphal outgrowth using a variety of growth tests."}
{"id": "PMID:1095689", "title": "Metoclopramide and pimozide in Parkinson's disease and levodopa-induced dyskinesias.", "content": "Metoclopramide is an antiemetic drug which occasionally produced acute dystonic reactions. Although known to interfere with central dopamine mechanisms, it is frequently used in Parkinson's disease to prevent levodopa-induced nausea and vomiting. In this study metoclopramide did not increase Parkinsonism or reduce levodopa-induced involuntary movements in patients with Parkinson's disease. Pimozide, by contrast, increased Parkinsonism and reduced involuntary movements. The capacity of metoclopramide to produce acute dyskinesias while being apparently free of Parkinsonism effects is pharmacologically unique and differentiates this drug from the phenothiazines and butyrophenones.", "contents": "Metoclopramide and pimozide in Parkinson's disease and levodopa-induced dyskinesias. Metoclopramide is an antiemetic drug which occasionally produced acute dystonic reactions. Although known to interfere with central dopamine mechanisms, it is frequently used in Parkinson's disease to prevent levodopa-induced nausea and vomiting. In this study metoclopramide did not increase Parkinsonism or reduce levodopa-induced involuntary movements in patients with Parkinson's disease. Pimozide, by contrast, increased Parkinsonism and reduced involuntary movements. The capacity of metoclopramide to produce acute dyskinesias while being apparently free of Parkinsonism effects is pharmacologically unique and differentiates this drug from the phenothiazines and butyrophenones."}
{"id": "PMID:1095693", "title": "Cerebrospinal fluid formation in ventricles and spinal subarachnoid space of the rhesus monkey.", "content": "The authors perfused rhesus monkeys from lateral ventricles to lumbar sac with an artificial cerebrospinal fluid (CSF) containing a Blue Dextran 2000 marker. Analysis of marker dilution at steady state showed ventricular CSF formation occurring at a rate of 28.3 plus or minus mu1/min. No significant CSF formation was found in the spinal subarachnoid space.", "contents": "Cerebrospinal fluid formation in ventricles and spinal subarachnoid space of the rhesus monkey. The authors perfused rhesus monkeys from lateral ventricles to lumbar sac with an artificial cerebrospinal fluid (CSF) containing a Blue Dextran 2000 marker. Analysis of marker dilution at steady state showed ventricular CSF formation occurring at a rate of 28.3 plus or minus mu1/min. No significant CSF formation was found in the spinal subarachnoid space."}
{"id": "PMID:1095694", "title": "Vasodilator system for the face.", "content": "The authors describe investigations in cats to delineate a vasodilator system to the face, which they undertook after a previous study showed that radiofrequency coagulation of the trigeminal ganglion produced a pronounced flush in the skin of the corresponding division. Results demonstrate a vasodilator system emerging from the brain stem with the facial nerve which, by way of the greater superficial petrosal nerve, reaches the trigeminal ganglion. There the fibers are distributed to each of the divisions of the fifth nerve; in addition, a moderate number of vasodilator fibers also appear to leave the brain stem directly with the trigeminal nerve. Vasodilator fibers also appear to leave the brain stem directly with the trigeminal nerve. Vasodilator effects were elicited by stereotaxic  stimulation of the facial and trigeminal nuclei in the brain stem. There is, therefore, a dual vasomotor control of the facial cutaneous vascular bed; the classical sympathetic vasoconstrictor system of the face is complemented by a vasodilator system capable of producing changes of equal but opposite amplitude in vessel caliber.", "contents": "Vasodilator system for the face. The authors describe investigations in cats to delineate a vasodilator system to the face, which they undertook after a previous study showed that radiofrequency coagulation of the trigeminal ganglion produced a pronounced flush in the skin of the corresponding division. Results demonstrate a vasodilator system emerging from the brain stem with the facial nerve which, by way of the greater superficial petrosal nerve, reaches the trigeminal ganglion. There the fibers are distributed to each of the divisions of the fifth nerve; in addition, a moderate number of vasodilator fibers also appear to leave the brain stem directly with the trigeminal nerve. Vasodilator fibers also appear to leave the brain stem directly with the trigeminal nerve. Vasodilator effects were elicited by stereotaxic  stimulation of the facial and trigeminal nuclei in the brain stem. There is, therefore, a dual vasomotor control of the facial cutaneous vascular bed; the classical sympathetic vasoconstrictor system of the face is complemented by a vasodilator system capable of producing changes of equal but opposite amplitude in vessel caliber."}
{"id": "PMID:1095695", "title": "Vernix caseosa meningitis and laryngeal stridor in an infant with myelomeningocele. Case report.", "content": "The authors present the case of an infant with laryngeal stridor, apneic spells, and an open lumbar myelomeningocele; granulomatous vernix caseosa meningitis was found at autopsy. The suggest that chemical meningitis which also involved the cranial nerves might be held partly responsible for the laryngeal stridor.", "contents": "Vernix caseosa meningitis and laryngeal stridor in an infant with myelomeningocele. Case report. The authors present the case of an infant with laryngeal stridor, apneic spells, and an open lumbar myelomeningocele; granulomatous vernix caseosa meningitis was found at autopsy. The suggest that chemical meningitis which also involved the cranial nerves might be held partly responsible for the laryngeal stridor."}
{"id": "PMID:1095696", "title": "Method for measuring brain tissue pressure. Response to alteration in pCO-2 systemic blood pressure, and middle cerebral artery occlusion.", "content": "The authors report a method for measuring total local brain tissue pressure (BTP) using a miniature catheter transducer stereotaxically introduced into the white matter of the cat's cerebrum. Quantitative rapid phasic pressure changes were satisfactorily demonstrated. Due to some drift of baseline of the transducers and inability to perform in vivo calibration, reliable long-term quantitative pressure measurements sometimes could not be studied. The BTP from each cerebral hemisphere and the cisternal pressure (CP) were monitored during alterations of pCO-2 and systemic blood pressure, and distilled H20 injection prior to and after right middle cerebral artery (MCA) ligation. The catheter transducers functioned well on chronic implantation for up to 6 weeks. Compared to the chronically implanted catheters, acutely implanted catheters responded identically except for drift. The response of intracranial pressure and CP to MCA occlusion, alterations in pCO2, and systemic blood pressure were similar. No BTP gradients appeared in response to MCA ligation, hypercapnia, hypertension, or progressive swelling of the resulting infarction.", "contents": "Method for measuring brain tissue pressure. Response to alteration in pCO-2 systemic blood pressure, and middle cerebral artery occlusion. The authors report a method for measuring total local brain tissue pressure (BTP) using a miniature catheter transducer stereotaxically introduced into the white matter of the cat's cerebrum. Quantitative rapid phasic pressure changes were satisfactorily demonstrated. Due to some drift of baseline of the transducers and inability to perform in vivo calibration, reliable long-term quantitative pressure measurements sometimes could not be studied. The BTP from each cerebral hemisphere and the cisternal pressure (CP) were monitored during alterations of pCO-2 and systemic blood pressure, and distilled H20 injection prior to and after right middle cerebral artery (MCA) ligation. The catheter transducers functioned well on chronic implantation for up to 6 weeks. Compared to the chronically implanted catheters, acutely implanted catheters responded identically except for drift. The response of intracranial pressure and CP to MCA occlusion, alterations in pCO2, and systemic blood pressure were similar. No BTP gradients appeared in response to MCA ligation, hypercapnia, hypertension, or progressive swelling of the resulting infarction."}
{"id": "PMID:1095699", "title": "Accumulation of gold in various organs of mice injected with gold thioglucose.", "content": "The response of either male or female mice to the administration of gold thioglucose (GTG) was observed in food intake, in body and organ weights, and in gold accumulation in respective organs. Male mice were more sensitive to GTG toxicity than females. During 103 days after GTG administration, the adipose tissues of the females and males increased in weight 15 times and 2.5 times as high as those of control mice, respectively. The weights of heart, lung, and brain were not affected by GTG administration. In the respective organs, gold concentrations, which were determined by neutron activation analysis, were high in liver, kidney, spleen, adrenal, and adipose tissue (especially in female) within a shorter time after the GTG injection. In various organs of nonobese mice, the concentrations of GTG were significantly less than those in the typical obese mice. Even after more than 100 days after injection, gold was detected in the respective organs. In the control mice without any GTG administration, no gold could be detected.", "contents": "Accumulation of gold in various organs of mice injected with gold thioglucose. The response of either male or female mice to the administration of gold thioglucose (GTG) was observed in food intake, in body and organ weights, and in gold accumulation in respective organs. Male mice were more sensitive to GTG toxicity than females. During 103 days after GTG administration, the adipose tissues of the females and males increased in weight 15 times and 2.5 times as high as those of control mice, respectively. The weights of heart, lung, and brain were not affected by GTG administration. In the respective organs, gold concentrations, which were determined by neutron activation analysis, were high in liver, kidney, spleen, adrenal, and adipose tissue (especially in female) within a shorter time after the GTG injection. In various organs of nonobese mice, the concentrations of GTG were significantly less than those in the typical obese mice. Even after more than 100 days after injection, gold was detected in the respective organs. In the control mice without any GTG administration, no gold could be detected."}
{"id": "PMID:1095700", "title": "Color essays.", "content": "Webster's New Collegiate Dictionary defines \"essay\" first as \"an effort to do something; attempt; trial.\" Only second it is defined as \"a literary composition, analytical or interpretative, dealing with its subject from a more or less limited or personal standpoint.\" This article is an essay (second meaning) about a few essays (first meaning) that have been made to understand color. Genesis, Aristotle, Plato, Newton, Palmer, Young, Helmholtz, Maxwell and Frederic Ives are represented.", "contents": "Color essays. Webster's New Collegiate Dictionary defines \"essay\" first as \"an effort to do something; attempt; trial.\" Only second it is defined as \"a literary composition, analytical or interpretative, dealing with its subject from a more or less limited or personal standpoint.\" This article is an essay (second meaning) about a few essays (first meaning) that have been made to understand color. Genesis, Aristotle, Plato, Newton, Palmer, Young, Helmholtz, Maxwell and Frederic Ives are represented."}
{"id": "PMID:1095703", "title": "A comparative study of three etching solutions: effects on enamel surface and adhesive-enamel interface.", "content": "The chemical treatment of enamel surfaces is an approach in obtaining increased bonding of dental materials to tooth srufaces. Three etching solutions commonly used at present are 50% H3PO4, 50% H3PO4 attenuated with 7% zinc oxide and 50% citric acid. The effects of these three conditioning solutions on normal and polished enamel surfaces and on the interfaces between untreated enamel and etched enamel surfaces were studied by scanning electron microscopy. The variable etching pattern observed with each of the acids made a comparative study difficult. This was further complicated by the dependence of the etching action on prism orientation on polished enamel surfaces. Additional factors that have to be considered are the presence of prismles enamel and perikymata. Surface profile recordings of polished and acid-etched polished enamel surfaces provided some quantitative measure of the etching action of the conditioning solutions. The 50% citric acid solution had the mildest etching action while 50% H3PO4 and 50% H3PO4 attentuated with 7% zinc oxide elicited comparable responses.", "contents": "A comparative study of three etching solutions: effects on enamel surface and adhesive-enamel interface. The chemical treatment of enamel surfaces is an approach in obtaining increased bonding of dental materials to tooth srufaces. Three etching solutions commonly used at present are 50% H3PO4, 50% H3PO4 attenuated with 7% zinc oxide and 50% citric acid. The effects of these three conditioning solutions on normal and polished enamel surfaces and on the interfaces between untreated enamel and etched enamel surfaces were studied by scanning electron microscopy. The variable etching pattern observed with each of the acids made a comparative study difficult. This was further complicated by the dependence of the etching action on prism orientation on polished enamel surfaces. Additional factors that have to be considered are the presence of prismles enamel and perikymata. Surface profile recordings of polished and acid-etched polished enamel surfaces provided some quantitative measure of the etching action of the conditioning solutions. The 50% citric acid solution had the mildest etching action while 50% H3PO4 and 50% H3PO4 attentuated with 7% zinc oxide elicited comparable responses."}
{"id": "PMID:1095704", "title": "Polymethylmethacrylate as a denture base material in service.", "content": "Assessments of the practical deficiencies of polymethylmethacrylate in service have been carried out by several workers. Some of these studies and one further one have been compared so as to seek any common factors, especially those concerned with the age of the appliance at failure. Times of vulnerability for different types of dentures have been demonstrated, and the scope for time-based studies in the laboratory and in service has been discussed.", "contents": "Polymethylmethacrylate as a denture base material in service. Assessments of the practical deficiencies of polymethylmethacrylate in service have been carried out by several workers. Some of these studies and one further one have been compared so as to seek any common factors, especially those concerned with the age of the appliance at failure. Times of vulnerability for different types of dentures have been demonstrated, and the scope for time-based studies in the laboratory and in service has been discussed."}
{"id": "PMID:1095705", "title": "Functional analysis of fixed bridges on abutment teeth with reduced periodontal support.", "content": "The present study examines the function of fixed bridges on abutment teeth with reduced but non-inflamed periodontal tissues. Eighteen patients with advanced periodontal breakdown and indication for oral rehabilitation received periodontol treatment and their dentitions were restored with fixed bridgework. The analysis of function performed 2-5 years after insertion of the reconstructions was made by (1) evaluation of the patients' opinion on chewing ability and function of the masticatory system; (2) clinical examination of the border movements of the mandible, the status of the temporomandivular joints and the chewing muscles, and (3) measurements of the bite force indifferent areas of the dentition. The results of the study show that the functional capacity of the type of extensive bridgework discussed is good and that the patients--in spite of severe loss of periodontal tissue--achieve bite force values that are almost comparable to those in individuals with natural teeth. Comparison of the function of fixed bridges and that of removable dentures is also discussed.", "contents": "Functional analysis of fixed bridges on abutment teeth with reduced periodontal support. The present study examines the function of fixed bridges on abutment teeth with reduced but non-inflamed periodontal tissues. Eighteen patients with advanced periodontal breakdown and indication for oral rehabilitation received periodontol treatment and their dentitions were restored with fixed bridgework. The analysis of function performed 2-5 years after insertion of the reconstructions was made by (1) evaluation of the patients' opinion on chewing ability and function of the masticatory system; (2) clinical examination of the border movements of the mandible, the status of the temporomandivular joints and the chewing muscles, and (3) measurements of the bite force indifferent areas of the dentition. The results of the study show that the functional capacity of the type of extensive bridgework discussed is good and that the patients--in spite of severe loss of periodontal tissue--achieve bite force values that are almost comparable to those in individuals with natural teeth. Comparison of the function of fixed bridges and that of removable dentures is also discussed."}
{"id": "PMID:1095706", "title": "Adaptation of silicate and Adaptic to the margins of cavities. A scanning electron microscope study.", "content": "The effects of etching of the enamel, on the adaptation of silicate and Adaptic to the cavity walls was examined with the scanning electron microscope. Replica methods were utilized to ensure that no shrinkage of the filling materials was caused by the vacuum in the instrument. It was shown that following etching of the cavity margins, Adaptic adhered to the enamel with absent, or minimal, fissure formation on one side of the filling, and some on the opposite side. The adaptation of silicate to the margins of the cavities was not improved by etching.", "contents": "Adaptation of silicate and Adaptic to the margins of cavities. A scanning electron microscope study. The effects of etching of the enamel, on the adaptation of silicate and Adaptic to the cavity walls was examined with the scanning electron microscope. Replica methods were utilized to ensure that no shrinkage of the filling materials was caused by the vacuum in the instrument. It was shown that following etching of the cavity margins, Adaptic adhered to the enamel with absent, or minimal, fissure formation on one side of the filling, and some on the opposite side. The adaptation of silicate to the margins of the cavities was not improved by etching."}
{"id": "PMID:1095707", "title": "Changes of cheek pressure during swallowing following expansion of the maxillary dental arch in repaired cleft palates.", "content": "Cheek pressure during swallowing was measured on six operated complete cleft lip and palate cases (4-8 years of age) before and after expansion of buccal segments of maxillary arch. As control, seven non-cleft subjects with acceptable occlusion of the same age level were used. Before expansion, the pressures in the cleft cases were much higher than the controls. Immediately after expansion, increase in pressure was observed in cases which received rapid expansion. However, a few months after expansion, most cases showed a decrease in pressure. This is explained as being due to the adaptation of the perioral musculature to the expanded dental arch and/or the disappearance of hyperactivity of the perioral musculature during swallowing.", "contents": "Changes of cheek pressure during swallowing following expansion of the maxillary dental arch in repaired cleft palates. Cheek pressure during swallowing was measured on six operated complete cleft lip and palate cases (4-8 years of age) before and after expansion of buccal segments of maxillary arch. As control, seven non-cleft subjects with acceptable occlusion of the same age level were used. Before expansion, the pressures in the cleft cases were much higher than the controls. Immediately after expansion, increase in pressure was observed in cases which received rapid expansion. However, a few months after expansion, most cases showed a decrease in pressure. This is explained as being due to the adaptation of the perioral musculature to the expanded dental arch and/or the disappearance of hyperactivity of the perioral musculature during swallowing."}
{"id": "PMID:1095708", "title": "Chalmers J. Lyons memorial lecture: Metal implants and the mandibular staple bone plate.", "content": "To briefly summarize our results, two of the five three-pin staples (40%) have survived and have functioned well for more than six years. Four of the five four-pin staples (80%) have survived five years or longer and are functioning well. Twenty-four of the 25 seven-pin staples (96%) are functioning well, some for a period of 4-1/2 years; a few in this group have been used a little less than a year. Our total percentage of survival with good function is 30 of 35, or 86%, which includes our pilot study of the three- and four-pin staples. A more significant statistic, however, might be those patients with staples who we feel have been free of any significant trouble; these are 27 of 35, or 77%. All of our patients have been subjected to peer review examination and grading by members of the oral surgery staff at Sinai Hospital. They were examined and graded according to three criteria. The first was clinical appearance which included firmness of the staple and clinical appearance of the gingiva (Fig 16). The second was radiographic evidence of bone loss along the transosteal pins and retentive pins (Fig 17). The third category was patient evaluation of improved function and stability of the lower denture (Fig 18). The peer review group used marks A, B, C, D, as excellent, good, fair, and failure. Four points were assigned to A, 3 points to B, 2 points to C, and 1 point to D, so that the grade averages for all three categories were as follows: clinical appearance, 3.6; radiographic appearance, 3.5; and patient evaluation, 3.9. The conclusion of the review group was that the mandibular staple was functioning well and that it was clinically useful. I have presented an overview of some of the characteristics of metallic implants that make them implantable devices, and some of the qualities of the host tissues that will allow placement of foreign material. We do not yet understand all of the factors that make alloplastic materials biocompatible or some of the reasons for rejection by the host. We need continued research in animal models to study new devices and new materials that might permit biologic attachments of the host to implant. We also need controlled clinical studies with peer review to establish reliability of those devices that are clinically useful. The mandibular staple has had an initial clinical trial that seems very promising and is worthy of continued clinical study and clinical application.", "contents": "Chalmers J. Lyons memorial lecture: Metal implants and the mandibular staple bone plate. To briefly summarize our results, two of the five three-pin staples (40%) have survived and have functioned well for more than six years. Four of the five four-pin staples (80%) have survived five years or longer and are functioning well. Twenty-four of the 25 seven-pin staples (96%) are functioning well, some for a period of 4-1/2 years; a few in this group have been used a little less than a year. Our total percentage of survival with good function is 30 of 35, or 86%, which includes our pilot study of the three- and four-pin staples. A more significant statistic, however, might be those patients with staples who we feel have been free of any significant trouble; these are 27 of 35, or 77%. All of our patients have been subjected to peer review examination and grading by members of the oral surgery staff at Sinai Hospital. They were examined and graded according to three criteria. The first was clinical appearance which included firmness of the staple and clinical appearance of the gingiva (Fig 16). The second was radiographic evidence of bone loss along the transosteal pins and retentive pins (Fig 17). The third category was patient evaluation of improved function and stability of the lower denture (Fig 18). The peer review group used marks A, B, C, D, as excellent, good, fair, and failure. Four points were assigned to A, 3 points to B, 2 points to C, and 1 point to D, so that the grade averages for all three categories were as follows: clinical appearance, 3.6; radiographic appearance, 3.5; and patient evaluation, 3.9. The conclusion of the review group was that the mandibular staple was functioning well and that it was clinically useful. I have presented an overview of some of the characteristics of metallic implants that make them implantable devices, and some of the qualities of the host tissues that will allow placement of foreign material. We do not yet understand all of the factors that make alloplastic materials biocompatible or some of the reasons for rejection by the host. We need continued research in animal models to study new devices and new materials that might permit biologic attachments of the host to implant. We also need controlled clinical studies with peer review to establish reliability of those devices that are clinically useful. The mandibular staple has had an initial clinical trial that seems very promising and is worthy of continued clinical study and clinical application."}
{"id": "PMID:1095711", "title": "Split portal flow in heterotopic hepatic transplantation.", "content": "Heterotopic hepatic transplantation was carried out in 31 pigs using three different methods of homograft revascularization. In one experimental group the host portal inflow was split between the homograft and native liver so that the graft was provided with intestinal-pancreatic effluent; the host liver with gastric-splenic venous blood. The liver grafts flourished in this environment. A small and variable degree of atrophy took place in the host liver. In the other two experimental groups in which the grafts were supplied with different kinds of systemic inflow only, atrophy invariably followed. Long-term homograft analysis was prevented by the intensity of immunologic rejection regardless of the location of the ectopic liver. The implications for clinical auxiliary liver transplantation are (1) heterotopic liver homografts must be supplied with a portal inflow from the host splanchnic bed, but (2) the total host splanchnic effluent is unnecessary. (3) The portal inflow must contain the pancreatic venous blood. (4) There is a criticial recipient--donor size relationship for successful portal revascularization in this type of auxiliary hepatic graft.", "contents": "Split portal flow in heterotopic hepatic transplantation. Heterotopic hepatic transplantation was carried out in 31 pigs using three different methods of homograft revascularization. In one experimental group the host portal inflow was split between the homograft and native liver so that the graft was provided with intestinal-pancreatic effluent; the host liver with gastric-splenic venous blood. The liver grafts flourished in this environment. A small and variable degree of atrophy took place in the host liver. In the other two experimental groups in which the grafts were supplied with different kinds of systemic inflow only, atrophy invariably followed. Long-term homograft analysis was prevented by the intensity of immunologic rejection regardless of the location of the ectopic liver. The implications for clinical auxiliary liver transplantation are (1) heterotopic liver homografts must be supplied with a portal inflow from the host splanchnic bed, but (2) the total host splanchnic effluent is unnecessary. (3) The portal inflow must contain the pancreatic venous blood. (4) There is a criticial recipient--donor size relationship for successful portal revascularization in this type of auxiliary hepatic graft."}
{"id": "PMID:1095712", "title": "Surgical closure of patent ductus arteriosus in the premature infant with respiratory distress.", "content": "During the period from February, 1971 to February, 1973, 30 premature infants underwent surgical ligation of patent ductus arteriosus. The gestational ages ranged from 25 to 36 wk (mean 30), and the birth weights ranged from 760 to 2010 g (mean 1274). The patients were divided into two groups on the basis of the indications for assisted ventilation. Group I consisted of 21 patients with severe hylanine membrane disease who required assisted ventilation during the first 2 days of life and could not be weaned off the respiratory by 10 days of age. Group II was composed of nine infants who required intermittent positive-pressure breathing after a mean age of 8 days because of repeated apneic spells secondary to uncontrollable heart failure. All infants in Group II survived the operation and left the hospital well. Fourteen of the 21 patients in Group I survived; the seven deaths were all due to underlying severe pulmonary disease (bronchopulmonary dysplasia). The value of PDA ligation in premature infants with uncontrollable heart failure is demonstrated in this study; this procedure also appears to be beneficial in neonates with severe respiratory distress syndrome.", "contents": "Surgical closure of patent ductus arteriosus in the premature infant with respiratory distress. During the period from February, 1971 to February, 1973, 30 premature infants underwent surgical ligation of patent ductus arteriosus. The gestational ages ranged from 25 to 36 wk (mean 30), and the birth weights ranged from 760 to 2010 g (mean 1274). The patients were divided into two groups on the basis of the indications for assisted ventilation. Group I consisted of 21 patients with severe hylanine membrane disease who required assisted ventilation during the first 2 days of life and could not be weaned off the respiratory by 10 days of age. Group II was composed of nine infants who required intermittent positive-pressure breathing after a mean age of 8 days because of repeated apneic spells secondary to uncontrollable heart failure. All infants in Group II survived the operation and left the hospital well. Fourteen of the 21 patients in Group I survived; the seven deaths were all due to underlying severe pulmonary disease (bronchopulmonary dysplasia). The value of PDA ligation in premature infants with uncontrollable heart failure is demonstrated in this study; this procedure also appears to be beneficial in neonates with severe respiratory distress syndrome."}
{"id": "PMID:1095714", "title": "The effects of antiepileptic drugs on estrogen-induced electrographic spike-wave discharge.", "content": "In locally anesthetized, paralyzed cats with bilateral conjugated estrogen (CE)-induced foci in sensory motor cortex, electrographic activity was characterized by 2 to 3 Hz spike and slow wave discharge. Commonly used anti-petit mal drugs (esthosuximide, trimethadione, acetazolamide and diazepam) all reduced CE-induced spike wave activity while diphenylhydantoin converted such activity into 9 to 12 Hz polyspike bursts separated by periods of interictal silence. Correlation appears to exist, therefore, between the ability of the drug to reduce CE-induced spike wave activity and its clinical utility in petit mal epilepsy. In addition to the above compounds, five drugs of less proven utility were evaluated. Of these, two benzodiazepine derivatives (clonazepam and clorazepate) were found to exert a potent and prolonged depressant action on CE-induced activity. The relation of CE to clinical petit mal epilepsy and the potential usefulness of CE as a laboratory model for the evaluation of anti-petit mal drugs are discussed.", "contents": "The effects of antiepileptic drugs on estrogen-induced electrographic spike-wave discharge. In locally anesthetized, paralyzed cats with bilateral conjugated estrogen (CE)-induced foci in sensory motor cortex, electrographic activity was characterized by 2 to 3 Hz spike and slow wave discharge. Commonly used anti-petit mal drugs (esthosuximide, trimethadione, acetazolamide and diazepam) all reduced CE-induced spike wave activity while diphenylhydantoin converted such activity into 9 to 12 Hz polyspike bursts separated by periods of interictal silence. Correlation appears to exist, therefore, between the ability of the drug to reduce CE-induced spike wave activity and its clinical utility in petit mal epilepsy. In addition to the above compounds, five drugs of less proven utility were evaluated. Of these, two benzodiazepine derivatives (clonazepam and clorazepate) were found to exert a potent and prolonged depressant action on CE-induced activity. The relation of CE to clinical petit mal epilepsy and the potential usefulness of CE as a laboratory model for the evaluation of anti-petit mal drugs are discussed."}
{"id": "PMID:1095715", "title": "The influence of aortic baroreceptors on venous tone in the perfused hind limb of the dog.", "content": "1. The aortic arch and both carotid sinuses were vascularly isolated and perfused. A hind limb was vascularly isolated and blood was pumped at constant flows into the femoral artery and the central end of a superficial metatarsal vein. 2. Large increases in aortic arch pressure resulted in decreases in arterial blood pressure, heart rate and femoral arterial perfusion pressure. The average response of the vein was a decrease of 11% in the pressure gradient between the perfused vein and the femoral vein. Similar responses were obtained when carotid sinus pressure was increased. 3. Crushing or cooling the lumbar sympathetic trunk caused responsed similar to those induced by increasing baroreceptor perfusion pressure. Stimulation at 1 HZ resulted in venous responses four times as great as the average reflex response, whereas frequencies of 2-5 Hz were required to produce changes in arterial resistance as great as those induced reflexly. 4. These experiments indicate, that although the large superficial veins of the dog's hind limb participate in the baroreceptor reflexes, the activities in the nerves supplying arterioles and veins must have been different.", "contents": "The influence of aortic baroreceptors on venous tone in the perfused hind limb of the dog. 1. The aortic arch and both carotid sinuses were vascularly isolated and perfused. A hind limb was vascularly isolated and blood was pumped at constant flows into the femoral artery and the central end of a superficial metatarsal vein. 2. Large increases in aortic arch pressure resulted in decreases in arterial blood pressure, heart rate and femoral arterial perfusion pressure. The average response of the vein was a decrease of 11% in the pressure gradient between the perfused vein and the femoral vein. Similar responses were obtained when carotid sinus pressure was increased. 3. Crushing or cooling the lumbar sympathetic trunk caused responsed similar to those induced by increasing baroreceptor perfusion pressure. Stimulation at 1 HZ resulted in venous responses four times as great as the average reflex response, whereas frequencies of 2-5 Hz were required to produce changes in arterial resistance as great as those induced reflexly. 4. These experiments indicate, that although the large superficial veins of the dog's hind limb participate in the baroreceptor reflexes, the activities in the nerves supplying arterioles and veins must have been different."}
{"id": "PMID:1095718", "title": "Alterations in secretory patterns following antrectomy in rats with Pavlov pouches.", "content": "1. In conscious rats provided with Pavlov pouches, with the antrum retained or resected,the gastric secretory response to various stimuli has been studied. Each acid secretory response was related to that obtained with maximal doses of methacholine and histamine in combination, presumed to reflect the maximal secretory capacity of the mucosa. 2. Three weeks after the operation, the maximal acid secretory capacity was 60 percent lower in the antrectomized than in the intact Pavlov pouch rats; the difference was still larger at 6 weeks and 3-5 months, owing to a gradual increase in the rats with the antrum retained. 3. Antrectomy reduced interdigestive secretion of acid to the same degree as the concomitant reduction in maximal secretory capacity. 4. Acid secretion in response to a maximal infusion of pentagastrin was reduced by about 50 percent at 3 and about 65 percent at 6 weeks after antrectomy. No significant difference was, however, noted between the antrectomized and intact rats when the responses were related to the maximal secretory capacity. The dose response curve to pentagastrin revealed a redcued responsiveness to submaximal doses of this agent following antrectomy. 5. The maximal acid secretory response to histamine was reduced after antrectomy, although the sensitivity to submaximal infusions of histamine appeared to be increased. 6. The mean secretroy output to 2-deoxy-D-glucose was reduced by about 65 percent and that to food by about 85 percent following antrectomy. 7. After antrectomy a background infusion of pentagastrin enhanced the secretory responses to 2-deoxy-D-glucose and to food but did not restore the responses to the levels in the intact rats. The feeding responses as related to the maximal secretory capacity were, however, similar in the two groups on infusing pentagastrin in the antrectomized rats. 8. Interdigestive secretion of pepsin was reduced by about 60 percent after antrectomy, while the peak response to 2-deoxy-Dglucose was about twice the interdigestive level in both groups. Pepsin secretion in response to food showed an increased secretion above the interdigestive level of longer duration in the antrectomized than in the intact Pavlov pouch rats. 9. The irreversibily reduced responsiveness of the gastric mucosa after antrectomy is discussed in relation to known morphological and biochemical changes.", "contents": "Alterations in secretory patterns following antrectomy in rats with Pavlov pouches. 1. In conscious rats provided with Pavlov pouches, with the antrum retained or resected,the gastric secretory response to various stimuli has been studied. Each acid secretory response was related to that obtained with maximal doses of methacholine and histamine in combination, presumed to reflect the maximal secretory capacity of the mucosa. 2. Three weeks after the operation, the maximal acid secretory capacity was 60 percent lower in the antrectomized than in the intact Pavlov pouch rats; the difference was still larger at 6 weeks and 3-5 months, owing to a gradual increase in the rats with the antrum retained. 3. Antrectomy reduced interdigestive secretion of acid to the same degree as the concomitant reduction in maximal secretory capacity. 4. Acid secretion in response to a maximal infusion of pentagastrin was reduced by about 50 percent at 3 and about 65 percent at 6 weeks after antrectomy. No significant difference was, however, noted between the antrectomized and intact rats when the responses were related to the maximal secretory capacity. The dose response curve to pentagastrin revealed a redcued responsiveness to submaximal doses of this agent following antrectomy. 5. The maximal acid secretory response to histamine was reduced after antrectomy, although the sensitivity to submaximal infusions of histamine appeared to be increased. 6. The mean secretroy output to 2-deoxy-D-glucose was reduced by about 65 percent and that to food by about 85 percent following antrectomy. 7. After antrectomy a background infusion of pentagastrin enhanced the secretory responses to 2-deoxy-D-glucose and to food but did not restore the responses to the levels in the intact rats. The feeding responses as related to the maximal secretory capacity were, however, similar in the two groups on infusing pentagastrin in the antrectomized rats. 8. Interdigestive secretion of pepsin was reduced by about 60 percent after antrectomy, while the peak response to 2-deoxy-Dglucose was about twice the interdigestive level in both groups. Pepsin secretion in response to food showed an increased secretion above the interdigestive level of longer duration in the antrectomized than in the intact Pavlov pouch rats. 9. The irreversibily reduced responsiveness of the gastric mucosa after antrectomy is discussed in relation to known morphological and biochemical changes."}
{"id": "PMID:1095720", "title": "Electrical characteristics of pancreatic islet cells.", "content": "1. The electrical properties of mouse pancreatic islet cells have been explored in vitro using a single intracellular micro-electrode for both voltage recording and current injection. 2. The frequency of spontaneous electrical activity induced in islet cells by concentrations of D-glucose greater than 2-8 mM was enhanced by depolarizing, and reduced by hyperpolarizing, current injection.  Post-stimulus inhibition and facilitation were also observed. 3. Intracellular current injection evoked a spike potential in Krebs solution containing a low D-glucose concentration (2-8mM), and in glucose-free, but not Ca-free solution.  Evoked spikes were observed in approximately 10% of the cell population impaled. 4. The relationship between the rate of rise of an evoked spike and membrane potential displacement by intracellular current injection a sigmoid curve suggesting the presence of an inactivation process in spike potential genesis. 5. High [K]o, 30-50mM, induced electrical activity rarely, and then only transiently, thereafter blocking it; conditioning hyperpolarizing current tended to restore spike activity. 6. D-600, 5 times 10-minus 5M, blocked the electrical activity induced by D-glucose, tolbutamide or current injection; these inhibitory effects were reversed by a threefold increase in [Ca]o to 7-68 mM. 7. From these results it is concluded that the electrical activity induced in islet cells by Dglucose, tolbutamide and current injection is due mainlu to Ca-2+ influx and is dependent on the level of the membrane potential", "contents": "Electrical characteristics of pancreatic islet cells. 1. The electrical properties of mouse pancreatic islet cells have been explored in vitro using a single intracellular micro-electrode for both voltage recording and current injection. 2. The frequency of spontaneous electrical activity induced in islet cells by concentrations of D-glucose greater than 2-8 mM was enhanced by depolarizing, and reduced by hyperpolarizing, current injection.  Post-stimulus inhibition and facilitation were also observed. 3. Intracellular current injection evoked a spike potential in Krebs solution containing a low D-glucose concentration (2-8mM), and in glucose-free, but not Ca-free solution.  Evoked spikes were observed in approximately 10% of the cell population impaled. 4. The relationship between the rate of rise of an evoked spike and membrane potential displacement by intracellular current injection a sigmoid curve suggesting the presence of an inactivation process in spike potential genesis. 5. High [K]o, 30-50mM, induced electrical activity rarely, and then only transiently, thereafter blocking it; conditioning hyperpolarizing current tended to restore spike activity. 6. D-600, 5 times 10-minus 5M, blocked the electrical activity induced by D-glucose, tolbutamide or current injection; these inhibitory effects were reversed by a threefold increase in [Ca]o to 7-68 mM. 7. From these results it is concluded that the electrical activity induced in islet cells by Dglucose, tolbutamide and current injection is due mainlu to Ca-2+ influx and is dependent on the level of the membrane potential"}
{"id": "PMID:1095721", "title": "Pancreatic islet cells: electrogenic and electrodiffusional control of membrane potential.", "content": "1. Responses of the membrane electrical characteristics of mouse pancreatic islet cells to ionic environmental changes have been used to assess the role of [Na]0 and [K]0 in the control of membrane potential, i.e. by electrodiffusion or via an electrogenic sodium pump. Islet cell electrical properties were measured in vitro with intracellular glass micro-electrodes. 2. Substitution of LiCl for extracellular NaCl did not change the islet cell membrane potential significantly in low (2.8 mM) glucose solutions, but readmission of NaCl caused a transient hyperpolarization (membrane potential maximum: -70 mV) in high glucose; when choline chloride was substituted for NaCl no hyperpolarization was observed on NaCl re-admission. 3. Superfusion with K-free solution gave no marked change in membrane potential during 30 min incubation in either low (2-8 mM) or high (28 mM) glucose concentrations but longer periods of exposure to K-free solutions caused progressive depolarization. 4. Readmission of K+ induced a transient hyperpolarization of up to 30 mV magnitude and 10 min duration in the presence of high (28 mM) but not low glucose (2-8 mM) concentrations. At the level of maximum hyperpolarization the membrane potential reached -60 mV, the electrical activity induced by the high glucose concentration being concurrently completely blocked. Replacement of [Cl]0 by isethionate accentuated these effects. 5. Ouabain, 10(-3) M, or a decrease in temperature from 37 to 7 degrees C depolarized the islet cells and blocked the transient hyperpolarization induced by readmission of K+. 6. Diphenylhydantoin, 1-5 times 10(-4) M, caused a significant hyperpolarization of the islet cells in low glucose (2-8 mM) and inhibited the electrical activity induced by high glucose (28 mM) or tolbutamide (0-7 mM). 7. It is concluded from these results that both an electrogenic and ionic component contribute to the membrane potential of the mouse pancreatic islet cell although electrodiffusional control normally predominates; acceleration of the Na-K exchange pump by diphenylhydantoin inhibits glucose-induced electrical activity. These findings are discussed in relation to the permeability characteristics of the islet cell membrane and the mechanism of insulin release.", "contents": "Pancreatic islet cells: electrogenic and electrodiffusional control of membrane potential. 1. Responses of the membrane electrical characteristics of mouse pancreatic islet cells to ionic environmental changes have been used to assess the role of [Na]0 and [K]0 in the control of membrane potential, i.e. by electrodiffusion or via an electrogenic sodium pump. Islet cell electrical properties were measured in vitro with intracellular glass micro-electrodes. 2. Substitution of LiCl for extracellular NaCl did not change the islet cell membrane potential significantly in low (2.8 mM) glucose solutions, but readmission of NaCl caused a transient hyperpolarization (membrane potential maximum: -70 mV) in high glucose; when choline chloride was substituted for NaCl no hyperpolarization was observed on NaCl re-admission. 3. Superfusion with K-free solution gave no marked change in membrane potential during 30 min incubation in either low (2-8 mM) or high (28 mM) glucose concentrations but longer periods of exposure to K-free solutions caused progressive depolarization. 4. Readmission of K+ induced a transient hyperpolarization of up to 30 mV magnitude and 10 min duration in the presence of high (28 mM) but not low glucose (2-8 mM) concentrations. At the level of maximum hyperpolarization the membrane potential reached -60 mV, the electrical activity induced by the high glucose concentration being concurrently completely blocked. Replacement of [Cl]0 by isethionate accentuated these effects. 5. Ouabain, 10(-3) M, or a decrease in temperature from 37 to 7 degrees C depolarized the islet cells and blocked the transient hyperpolarization induced by readmission of K+. 6. Diphenylhydantoin, 1-5 times 10(-4) M, caused a significant hyperpolarization of the islet cells in low glucose (2-8 mM) and inhibited the electrical activity induced by high glucose (28 mM) or tolbutamide (0-7 mM). 7. It is concluded from these results that both an electrogenic and ionic component contribute to the membrane potential of the mouse pancreatic islet cell although electrodiffusional control normally predominates; acceleration of the Na-K exchange pump by diphenylhydantoin inhibits glucose-induced electrical activity. These findings are discussed in relation to the permeability characteristics of the islet cell membrane and the mechanism of insulin release."}
{"id": "PMID:1095722", "title": "Pancreatic islet cells: effects of monosaccharides, glycolytic intermediates and metabolic inhibitors on membrane potential and electrical activity.", "content": "1. The effects of monosaccharides, glycolytic intermediates, metabolic inhibitors and anxia, have been studied on the membrane electrical activity of mouse pancreatic islet cells in vitro using a single intracellular micro-electrode for both voltage recording and current injection. 2. In addition to D-glucose (28mM), D-mannose (16-6mM), and L-leucin (10mM), the substances D-glyceraldehyde (11mM), and acetoacetate (20 mM), induced action potentials in islet cells but other glucos analogues and metabolic intermediates including L-glucose dod not. 3. Mannoheptulose 20 mM), but not D-galactose or 2-deoxy-D-glucose, antagonized the electrical activity induced in islet cells by D-glucose, 28mM.  Prior treatment of the cells with mannoheptulose caused them to hyperpolarize and completely prevented the appearance of electrical activity on subsequent exposure to D-glucose. 4. Electrical activity induced by D0glucose 28mM, was progressively inhibited by phloridzin, 10mM, if the cells were exposed to D-glucose and inhibitor simultaneously, and abolished on pretreatment with inhibitor for 30-60 min.  Phloridzin also caused depolarization of the islet cells which was independent of extracellular glucose. 5. Anoxia completely blocked the electrical activity induced by glucose but not that evoked by D-glyceraldehyde, L-leucine, tolbutamide or glibenclamide. 6. Iodoacetic acid, 5 mM, rapidly blocked glucose-induced electrical activity whilst that elicited by tolbutamide was relatively resistant to inhibition. 7. The nature and possible location of the glucoreceptor in pancreatic islet cells is discussed in relation to the origin and functional significance of glucose-induced electrical activity and insulin secretion.", "contents": "Pancreatic islet cells: effects of monosaccharides, glycolytic intermediates and metabolic inhibitors on membrane potential and electrical activity. 1. The effects of monosaccharides, glycolytic intermediates, metabolic inhibitors and anxia, have been studied on the membrane electrical activity of mouse pancreatic islet cells in vitro using a single intracellular micro-electrode for both voltage recording and current injection. 2. In addition to D-glucose (28mM), D-mannose (16-6mM), and L-leucin (10mM), the substances D-glyceraldehyde (11mM), and acetoacetate (20 mM), induced action potentials in islet cells but other glucos analogues and metabolic intermediates including L-glucose dod not. 3. Mannoheptulose 20 mM), but not D-galactose or 2-deoxy-D-glucose, antagonized the electrical activity induced in islet cells by D-glucose, 28mM.  Prior treatment of the cells with mannoheptulose caused them to hyperpolarize and completely prevented the appearance of electrical activity on subsequent exposure to D-glucose. 4. Electrical activity induced by D0glucose 28mM, was progressively inhibited by phloridzin, 10mM, if the cells were exposed to D-glucose and inhibitor simultaneously, and abolished on pretreatment with inhibitor for 30-60 min.  Phloridzin also caused depolarization of the islet cells which was independent of extracellular glucose. 5. Anoxia completely blocked the electrical activity induced by glucose but not that evoked by D-glyceraldehyde, L-leucine, tolbutamide or glibenclamide. 6. Iodoacetic acid, 5 mM, rapidly blocked glucose-induced electrical activity whilst that elicited by tolbutamide was relatively resistant to inhibition. 7. The nature and possible location of the glucoreceptor in pancreatic islet cells is discussed in relation to the origin and functional significance of glucose-induced electrical activity and insulin secretion."}
{"id": "PMID:1095738", "title": "Spermatogenesis in mature and regressed testes of the vole (Microtus agrestis).", "content": "Eight stages of spermatogenesis, each with a characteristic frequency and germ cell association could be recognized in the vole. There was no difference between laboratory bred and field animals in the frequency of the stages. Counts of the different types of germ cell showed that there was considerable cell loss during spermatogonial mitotic and spermatocytic meiotic divisions. Only 60% of germ cells became spermatozoa in sexually mature animals, and 19% in the regressing testes of voles exposed to short photoperiods. Animals with regressed testes probably have lowered circulating levels of gonadotrophins and testicular hormones, so that the greater germ loss suggests the importance of these hormones in the regulation of germ cell wastage. From the cell counts in mature animals, a scheme of cell divisions has been suggested by which spermatogonia produce progressively more highly differentiated germ cells while continuing to perpetuate stem cells.", "contents": "Spermatogenesis in mature and regressed testes of the vole (Microtus agrestis). Eight stages of spermatogenesis, each with a characteristic frequency and germ cell association could be recognized in the vole. There was no difference between laboratory bred and field animals in the frequency of the stages. Counts of the different types of germ cell showed that there was considerable cell loss during spermatogonial mitotic and spermatocytic meiotic divisions. Only 60% of germ cells became spermatozoa in sexually mature animals, and 19% in the regressing testes of voles exposed to short photoperiods. Animals with regressed testes probably have lowered circulating levels of gonadotrophins and testicular hormones, so that the greater germ loss suggests the importance of these hormones in the regulation of germ cell wastage. From the cell counts in mature animals, a scheme of cell divisions has been suggested by which spermatogonia produce progressively more highly differentiated germ cells while continuing to perpetuate stem cells."}
{"id": "PMID:1095739", "title": "Control of time of ovulation in heifers treated with progesterone and gonadotrophin-releasing hormone.", "content": "The time of ovulation was determined in twenty-nine heifers by laparoscopy following treatment with progesterone. In addition, twenty-five of the animals received 100 mug Gn-RH 30 hr after the end of the progesterone treatment. Only eight of the heifers treated with Gn-RH ovulated. This contrasted with results obtained following examination of ovaries after slaughter in similar animals treated in identical fashion. Heifers which were treated with progesterone for 12 days and then slaughtered had not begun to ovulate 65 hr after the end of treatment, but five of eleven animals had ovulated 4 hr later. When Gn-RH was given, ovulation had started 60 hr after the end of progesterpme treatment, 60% of heifers had ovulated at 65 hr and 90% at 69 hr. Treatment with Gn-RH can synchronize and hasten the time of ovulation in heifers given progesterone. Possible reasons for the discrepancy between the results obtained following laparoscopy and slaughter are discussed.", "contents": "Control of time of ovulation in heifers treated with progesterone and gonadotrophin-releasing hormone. The time of ovulation was determined in twenty-nine heifers by laparoscopy following treatment with progesterone. In addition, twenty-five of the animals received 100 mug Gn-RH 30 hr after the end of the progesterone treatment. Only eight of the heifers treated with Gn-RH ovulated. This contrasted with results obtained following examination of ovaries after slaughter in similar animals treated in identical fashion. Heifers which were treated with progesterone for 12 days and then slaughtered had not begun to ovulate 65 hr after the end of treatment, but five of eleven animals had ovulated 4 hr later. When Gn-RH was given, ovulation had started 60 hr after the end of progesterpme treatment, 60% of heifers had ovulated at 65 hr and 90% at 69 hr. Treatment with Gn-RH can synchronize and hasten the time of ovulation in heifers given progesterone. Possible reasons for the discrepancy between the results obtained following laparoscopy and slaughter are discussed."}
{"id": "PMID:1095741", "title": "An interactive computer program for studying fetal electroencephalograms.", "content": "Fetal electroencephalogram (FEEG), recorded during labor, produces very large volumes of data for visual interpretation. An established terminology, developed for the interpretation of neonatal electroencephalogram, has been found to be useful for visual pattern recognition of FEEG. A program, which identifies FEEG patterns within ten second epochs and provides direct comparison between visual and programmed analysis, has been developed using an interactive computer system. This program provides 85-90 percent consistency with visual interpretation.", "contents": "An interactive computer program for studying fetal electroencephalograms. Fetal electroencephalogram (FEEG), recorded during labor, produces very large volumes of data for visual interpretation. An established terminology, developed for the interpretation of neonatal electroencephalogram, has been found to be useful for visual pattern recognition of FEEG. A program, which identifies FEEG patterns within ten second epochs and provides direct comparison between visual and programmed analysis, has been developed using an interactive computer system. This program provides 85-90 percent consistency with visual interpretation."}
{"id": "PMID:1095742", "title": "Male sterilization in El Salvador: A preliminary report.", "content": "The expectation that male sterilization would not be an acceptable method of fertility control to the Latin American male has not been upheld in the initial phases of male sterilization programs in Colombia, Costa Rica, Guatemala, and El Salvador. On the contrary, in all of these programs and also at the Asociaci\u00f3n Demogr\u00e1fica Salvadore\u00f1a, a free-standing, non-profit family planning clinic in San Salvador, the initial success has been impressive, with 180 men accepting sterilization during the first two years (February, 1972 to February, 1974). All sterilizations were performed by excising a 10-15 mm segment of each vas and ligating each vas end. Procedures were performed on an outpatient basis using a local anesthetic. Follow-up examinations were conducted at one week and three months after the procedure. Although complications were reported by 24.0% of the subjects at the one-week follow-up, the complications were minor: most were scrotal infections not requiring antibiotic therapy. Rates of complications were low for subjects returning for a three-month examination and semen test. Only one subject had a positive semen test.", "contents": "Male sterilization in El Salvador: A preliminary report. The expectation that male sterilization would not be an acceptable method of fertility control to the Latin American male has not been upheld in the initial phases of male sterilization programs in Colombia, Costa Rica, Guatemala, and El Salvador. On the contrary, in all of these programs and also at the Asociaci\u00f3n Demogr\u00e1fica Salvadore\u00f1a, a free-standing, non-profit family planning clinic in San Salvador, the initial success has been impressive, with 180 men accepting sterilization during the first two years (February, 1972 to February, 1974). All sterilizations were performed by excising a 10-15 mm segment of each vas and ligating each vas end. Procedures were performed on an outpatient basis using a local anesthetic. Follow-up examinations were conducted at one week and three months after the procedure. Although complications were reported by 24.0% of the subjects at the one-week follow-up, the complications were minor: most were scrotal infections not requiring antibiotic therapy. Rates of complications were low for subjects returning for a three-month examination and semen test. Only one subject had a positive semen test."}
{"id": "PMID:1095744", "title": "A quality filtering system for medical literature.", "content": "An algorithmic method for selecting quality journal articles is presented. The procedures are based on formalizations of existing evaluation mechanisms in scientific publication. The method was actually applied to the journal literature of the pharmacology of cardiac arrhythmias for the period 1967 to 1970. A comparison with the selected bibliographies of four recent publications showed that the method approximated some of the selections made by conventional subjective judgment.", "contents": "A quality filtering system for medical literature. An algorithmic method for selecting quality journal articles is presented. The procedures are based on formalizations of existing evaluation mechanisms in scientific publication. The method was actually applied to the journal literature of the pharmacology of cardiac arrhythmias for the period 1967 to 1970. A comparison with the selected bibliographies of four recent publications showed that the method approximated some of the selections made by conventional subjective judgment."}
{"id": "PMID:1095748", "title": "Urinary and faecal Escherichia coli O-sero-groups in symptomatic urinary-tract infection and asymptomatic bacteriuria.", "content": "The degree of correspondence between urinary and faecal Escherichia coli O-groups has been assessed in non-pregnant women with symptomatic urinary-tract infection or asymptomatic bacteriuria. In 20 of 26 patients with symptomatic urinary tract infection E. coli of the same O-groups as that of the urinary infecting strain was also present in the patient's faecal flora of only five of 25 patients with asymptomatic bacteriuria. This finding indicates that the majority of episodes of symptomatic urinary tract infection in non-pregnant women are not preceded by a significant period of asymptomatic bacteriuria. E. coli O6 showed correspondence between urinary and faecal isolates more frequently than did other O-groups, but it had a relatively low prevalence in the faecal flora of patients with urinary-tract infection caused by E. coli of other O-groups. This finding lends support to previous suggestions that E. coli O6 may be especially pathogenic for the urinary tract.", "contents": "Urinary and faecal Escherichia coli O-sero-groups in symptomatic urinary-tract infection and asymptomatic bacteriuria. The degree of correspondence between urinary and faecal Escherichia coli O-groups has been assessed in non-pregnant women with symptomatic urinary-tract infection or asymptomatic bacteriuria. In 20 of 26 patients with symptomatic urinary tract infection E. coli of the same O-groups as that of the urinary infecting strain was also present in the patient's faecal flora of only five of 25 patients with asymptomatic bacteriuria. This finding indicates that the majority of episodes of symptomatic urinary tract infection in non-pregnant women are not preceded by a significant period of asymptomatic bacteriuria. E. coli O6 showed correspondence between urinary and faecal isolates more frequently than did other O-groups, but it had a relatively low prevalence in the faecal flora of patients with urinary-tract infection caused by E. coli of other O-groups. This finding lends support to previous suggestions that E. coli O6 may be especially pathogenic for the urinary tract."}
{"id": "PMID:1095749", "title": "Immunological studies on leprosy: separation and evaluation of the antigens of Mycobacterium leprae.", "content": "Chromatographically separated antigens of Mycobacterium leprae were tested for their ability to elicit skin reactions in guinea-pigs sensitised with homologous and heterologous mycobacteria. Of the three antigen-positive fractions obtained, one showed specific activity and the other two cross-reactivity, as indicated by studies of hypersensitivity and passive cutaneous anaphylaxis. The fraction exhibiting specificity contained only one antigen, which was protein in nature, whereas the other two fractions contained more than one antigen and possessed both protein and polysaccharide constituents. Because the single-antigen-containing fraction showed both positive skin and PCA reactivity, the suggestion is made that this fraction may contain either an antigen with two determinants or may contain two antigens that are not easily distinguishable by immunodiffusion methods.", "contents": "Immunological studies on leprosy: separation and evaluation of the antigens of Mycobacterium leprae. Chromatographically separated antigens of Mycobacterium leprae were tested for their ability to elicit skin reactions in guinea-pigs sensitised with homologous and heterologous mycobacteria. Of the three antigen-positive fractions obtained, one showed specific activity and the other two cross-reactivity, as indicated by studies of hypersensitivity and passive cutaneous anaphylaxis. The fraction exhibiting specificity contained only one antigen, which was protein in nature, whereas the other two fractions contained more than one antigen and possessed both protein and polysaccharide constituents. Because the single-antigen-containing fraction showed both positive skin and PCA reactivity, the suggestion is made that this fraction may contain either an antigen with two determinants or may contain two antigens that are not easily distinguishable by immunodiffusion methods."}
{"id": "PMID:1095750", "title": "Assessment of a new selective medium for the isolation of Salmonellae.", "content": "Shanson's medium, a MacConkey-type agar containing added inhibitors, was compared with deoxycholate-citrate agar for the examination of faeces. The isolation rate of samonellae on the two media was similar, but Shanson's medium was the more selective; this meant that the amount of work required to produce a result was reduced.", "contents": "Assessment of a new selective medium for the isolation of Salmonellae. Shanson's medium, a MacConkey-type agar containing added inhibitors, was compared with deoxycholate-citrate agar for the examination of faeces. The isolation rate of samonellae on the two media was similar, but Shanson's medium was the more selective; this meant that the amount of work required to produce a result was reduced."}
{"id": "PMID:1095751", "title": "Reversible permeability changes in the membrane of a yeast cell sugar compartment.", "content": "Sorbose uptake by Saccharomyces cerevisiae was increased 40 to 60% by glucose and other metabolizable sugars. Neither growth nor binding accounted for the increased uptake. However, accessibility of a restrictive intracellular compartment was increased as shown by counterflow and efflux measurements. Efflux from the compartment was more than doubled by glucose. This effect was reversed by washing and was prevented by iodoacetic acid and other inhibitors, but not by cycloheximide. No evidence was found for a facilitated transport system in the compartment membrane such as exists in the external cell membrane.  It was concluded that sorbose crosses the compartment membrane by simple diffusion and that a reaction requiring the metabolism of sugars increases the permeability of the membrane. Arabinose and fucose entered and were lost from the compartment like sorbose, whereas dimethylsulfoxide was unaffected by the compartment. All three of these later compounds were bound by the cells when glucose was available in uptake suspensions. Binding was prevented by iodoacetic acid, but not by cycloheximide.", "contents": "Reversible permeability changes in the membrane of a yeast cell sugar compartment. Sorbose uptake by Saccharomyces cerevisiae was increased 40 to 60% by glucose and other metabolizable sugars. Neither growth nor binding accounted for the increased uptake. However, accessibility of a restrictive intracellular compartment was increased as shown by counterflow and efflux measurements. Efflux from the compartment was more than doubled by glucose. This effect was reversed by washing and was prevented by iodoacetic acid and other inhibitors, but not by cycloheximide. No evidence was found for a facilitated transport system in the compartment membrane such as exists in the external cell membrane.  It was concluded that sorbose crosses the compartment membrane by simple diffusion and that a reaction requiring the metabolism of sugars increases the permeability of the membrane. Arabinose and fucose entered and were lost from the compartment like sorbose, whereas dimethylsulfoxide was unaffected by the compartment. All three of these later compounds were bound by the cells when glucose was available in uptake suspensions. Binding was prevented by iodoacetic acid, but not by cycloheximide."}
{"id": "PMID:1095752", "title": "Single atom image contrast: conventional dark-field and bright-field electron microscopy.", "content": "Single atom inage intensities are calculated for bright-field and various dark-field modes, including the ideal beam stop, a wire beam stop, tilted illumination and a displaced aperture. Comparisons of scattering amplitudes and elastic scattering cross-sections are made with different object potentials and scattering formulations. The image contrast for one mercury atom (Z equals 80) on a column of carbon atoms (Z equals 6) as the substrate is also discussed for both the bright-field and the various dark-field situations.", "contents": "Single atom image contrast: conventional dark-field and bright-field electron microscopy. Single atom inage intensities are calculated for bright-field and various dark-field modes, including the ideal beam stop, a wire beam stop, tilted illumination and a displaced aperture. Comparisons of scattering amplitudes and elastic scattering cross-sections are made with different object potentials and scattering formulations. The image contrast for one mercury atom (Z equals 80) on a column of carbon atoms (Z equals 6) as the substrate is also discussed for both the bright-field and the various dark-field situations."}
{"id": "PMID:1095775", "title": "V. Effect of enzymes and temperature on the serological activity of the antigen of Fasciola gigantica.", "content": "The partially purified extract of Fasciola gigantica was incubated with enzyme pepsin and samples were taken at different time intervals from 0-24 hours, and tested intradermally for diagnosing bilharziasis. The enzymatic degradation of the protein molecule results in the progressive decrease and eventually the complete disappearance of serological activity. This showed that the protein portion of the antigen was deemed necessary for its activity in intradermal tests for diagnosing schistosomiasis. Amylase, even at a concentration of 5 per cent, did not influence the activity of the partially purified extract of Fasciola gigantica. This showed that the carbohydrate portion of the antigen has no effect on serological activity. The partially purified extract of Fasciola gigantica was subjected to heating at 37 degrees C, 50 degrees C, and 60 degrees C for periods of two, six and 24 hours, and tested intradermally for diagnosing schistosomiasis. Heating decreased the reactivity of the supernatant liquid of the protein solution but did not completely inactivate the serologically active component which is fairly stable at 50 degrees C. Temperatures higher than 50 degrees C started to affect the serological activity of the protein solution. The higher the temperature, the shorter is the time required for the inactivation of the protein. This shows that the serologically active component is protein and not polysaccharide which resists much higher temperatures.", "contents": "V. Effect of enzymes and temperature on the serological activity of the antigen of Fasciola gigantica. The partially purified extract of Fasciola gigantica was incubated with enzyme pepsin and samples were taken at different time intervals from 0-24 hours, and tested intradermally for diagnosing bilharziasis. The enzymatic degradation of the protein molecule results in the progressive decrease and eventually the complete disappearance of serological activity. This showed that the protein portion of the antigen was deemed necessary for its activity in intradermal tests for diagnosing schistosomiasis. Amylase, even at a concentration of 5 per cent, did not influence the activity of the partially purified extract of Fasciola gigantica. This showed that the carbohydrate portion of the antigen has no effect on serological activity. The partially purified extract of Fasciola gigantica was subjected to heating at 37 degrees C, 50 degrees C, and 60 degrees C for periods of two, six and 24 hours, and tested intradermally for diagnosing schistosomiasis. Heating decreased the reactivity of the supernatant liquid of the protein solution but did not completely inactivate the serologically active component which is fairly stable at 50 degrees C. Temperatures higher than 50 degrees C started to affect the serological activity of the protein solution. The higher the temperature, the shorter is the time required for the inactivation of the protein. This shows that the serologically active component is protein and not polysaccharide which resists much higher temperatures."}
{"id": "PMID:1095776", "title": "Chloroquine resistant malaria in West Malaysia.", "content": "Chloroquine resistance is a well established entity in South East Asia, and presents a problem of increasing importance. Strains of P. falciparum resistant to chloroquine have also been found to be resistant to amodiaquine and a combination of pyrimethamine and sulphadoxine. Knowledge of the drug sensitivity of the strains of malaria parasite in a given locality is important so that the right choice of drugs can be made in treatment of the disease. The treatment of chloroquine resistant malaria in West Malaysia is a subject of another paper but suffice it to say that increased doses of chloroquine have still been found to be effective in treating many cases of falciparum malaria from areas of chloroquine resistance.", "contents": "Chloroquine resistant malaria in West Malaysia. Chloroquine resistance is a well established entity in South East Asia, and presents a problem of increasing importance. Strains of P. falciparum resistant to chloroquine have also been found to be resistant to amodiaquine and a combination of pyrimethamine and sulphadoxine. Knowledge of the drug sensitivity of the strains of malaria parasite in a given locality is important so that the right choice of drugs can be made in treatment of the disease. The treatment of chloroquine resistant malaria in West Malaysia is a subject of another paper but suffice it to say that increased doses of chloroquine have still been found to be effective in treating many cases of falciparum malaria from areas of chloroquine resistance."}
{"id": "PMID:1095777", "title": "Phospholipase activity in bacteriophage-infected Escherichia. II. Activation of phospholipase by T4 ghost infection.", "content": "The release of free fatty acids from the phospholipids of Escherichia coli is initiated immediately after the attachment of T4 ghosts. A similar accumulation of free fatty acids is observed if the cells are infected with T4 phage in the presence of chloramphenicol or puromycin. An early accumulation of free fatty acids, however, is not observed in T4 infections in which chloramphenicol or puromycin are not present, nor does it occur if the E. coli are infected with T4 phage before ghost infection, suggesting that phage products can prevent the phospholipid deacylation. If E. coli is infected with T4 ghosts before T4 phage infection, the accumulation of free fatty acids is not suppressed. When phospholipase-deficient E, coli are infected with T4 ghosts the appearance of free fatty acids is not observed, suggesting that T4 ghost attachment can activate the phospholipase of wild-type E. coli. Although the formation of free fatty acid apparently is a consequence of activation of the detergent-resistant phospholipase of the outer membrane, it is not observed in mutants deficient in the detergent-sensitive phospholipase.", "contents": "Phospholipase activity in bacteriophage-infected Escherichia. II. Activation of phospholipase by T4 ghost infection. The release of free fatty acids from the phospholipids of Escherichia coli is initiated immediately after the attachment of T4 ghosts. A similar accumulation of free fatty acids is observed if the cells are infected with T4 phage in the presence of chloramphenicol or puromycin. An early accumulation of free fatty acids, however, is not observed in T4 infections in which chloramphenicol or puromycin are not present, nor does it occur if the E. coli are infected with T4 phage before ghost infection, suggesting that phage products can prevent the phospholipid deacylation. If E. coli is infected with T4 ghosts before T4 phage infection, the accumulation of free fatty acids is not suppressed. When phospholipase-deficient E, coli are infected with T4 ghosts the appearance of free fatty acids is not observed, suggesting that T4 ghost attachment can activate the phospholipase of wild-type E. coli. Although the formation of free fatty acid apparently is a consequence of activation of the detergent-resistant phospholipase of the outer membrane, it is not observed in mutants deficient in the detergent-sensitive phospholipase."}
{"id": "PMID:1095778", "title": "Synthesis of viral and rRNA in bacteriophage R17 infection of a stringent strain of Escherichia coli.", "content": "A previous paper (1973) indicated that infection with bacteriophage R17 permits the synthesis of RNA and spermidine in Escherichia coli (CP78 in the absence of the exogenous essential amino acid, arginine. We have now isolated RNA formed under such conditions and analyzed the newly synthesized species by agarose-acrylamide electrophoresis. It has been shown that infection of the stringent cells in the absence of exogenous arginine resulted in a marked incorporation of uracil into rRNA, as well as into R17 RNA. It was shown that, although the organism was nonauxotrophic for uracil, addition of [-14C]uracil resulted in the rapid formation of TUP, the specific radioactivity of which approached that of the exogenous uracil. This indicated that the incorporation of exogenous uracil into rRNA in R17 infection of the stringent strain reflected a true stimulated synthesis of this nucleic acid. Infection of the essentially isogenic relaxed strain, CP79, under the same conditions inhibited the RNA synthesis to a much less extent than the inhibition caused during the normal infection. These observations provide another example of the close correlation between synthesis of spermidine and of host RNA, even in cells infected by an RNA bacteriophage.", "contents": "Synthesis of viral and rRNA in bacteriophage R17 infection of a stringent strain of Escherichia coli. A previous paper (1973) indicated that infection with bacteriophage R17 permits the synthesis of RNA and spermidine in Escherichia coli (CP78 in the absence of the exogenous essential amino acid, arginine. We have now isolated RNA formed under such conditions and analyzed the newly synthesized species by agarose-acrylamide electrophoresis. It has been shown that infection of the stringent cells in the absence of exogenous arginine resulted in a marked incorporation of uracil into rRNA, as well as into R17 RNA. It was shown that, although the organism was nonauxotrophic for uracil, addition of [-14C]uracil resulted in the rapid formation of TUP, the specific radioactivity of which approached that of the exogenous uracil. This indicated that the incorporation of exogenous uracil into rRNA in R17 infection of the stringent strain reflected a true stimulated synthesis of this nucleic acid. Infection of the essentially isogenic relaxed strain, CP79, under the same conditions inhibited the RNA synthesis to a much less extent than the inhibition caused during the normal infection. These observations provide another example of the close correlation between synthesis of spermidine and of host RNA, even in cells infected by an RNA bacteriophage."}
{"id": "PMID:1095779", "title": "Chloramphenicol stimulation of lysogeny by lambda regulatory mutants.", "content": "Inhibition of protein synthesis in Escherichia coli by amino acid starvation or chloramphenicol addition increases the frequency of lysogeny by lambda phage two- to fourfold. Lambda cIII mutants, which normally lysogenize at very low frequencies, lysogenize at very high frequencies in the presence of chloramphenicol.", "contents": "Chloramphenicol stimulation of lysogeny by lambda regulatory mutants. Inhibition of protein synthesis in Escherichia coli by amino acid starvation or chloramphenicol addition increases the frequency of lysogeny by lambda phage two- to fourfold. Lambda cIII mutants, which normally lysogenize at very low frequencies, lysogenize at very high frequencies in the presence of chloramphenicol."}
{"id": "PMID:1095780", "title": "The absence of vesicoureteral reflux in the neonate with myelodysplasia.", "content": "Urologic evaluation of 21 newborns with myelomeningoceles revealed a rarity of infection and an absence of vesicoureteral reflux on cystourethrography. An hypothesis has been advanced to explain the absence of reflux and the high incidence of reflux reported in older children with myelomeningoceles. The absence of reflux in these neonates indicates that reflux in children with myelomenigoceles is not caused by a primary congenital ureterovesical junction abnormality but rather is secondary to infection and/or progressive bladder dysfunction.", "contents": "The absence of vesicoureteral reflux in the neonate with myelodysplasia. Urologic evaluation of 21 newborns with myelomeningoceles revealed a rarity of infection and an absence of vesicoureteral reflux on cystourethrography. An hypothesis has been advanced to explain the absence of reflux and the high incidence of reflux reported in older children with myelomeningoceles. The absence of reflux in these neonates indicates that reflux in children with myelomenigoceles is not caused by a primary congenital ureterovesical junction abnormality but rather is secondary to infection and/or progressive bladder dysfunction."}
{"id": "PMID:1095781", "title": "Vesicoureteral reflux in babies with myelomeningocele.", "content": "Reflux was present at birth in 24 per cent of 100 babies with meningomyelocele, indicating a marked tendency toward spontaneous improvement in infancy. Reflux starts in some children after birth but the number is too small to draw definite conclusions on the cause of this onset. Of the children with myelomeningocele 16 per cent had asymptomatic infection of the urine on 1 occasion only and 33 per cent had recurrent infections. There was a greater incidence of upper tract anomalies in patients with a high level spinal lesion.", "contents": "Vesicoureteral reflux in babies with myelomeningocele. Reflux was present at birth in 24 per cent of 100 babies with meningomyelocele, indicating a marked tendency toward spontaneous improvement in infancy. Reflux starts in some children after birth but the number is too small to draw definite conclusions on the cause of this onset. Of the children with myelomeningocele 16 per cent had asymptomatic infection of the urine on 1 occasion only and 33 per cent had recurrent infections. There was a greater incidence of upper tract anomalies in patients with a high level spinal lesion."}
{"id": "PMID:1095782", "title": "Spiral advancement skin flap for stomal revision.", "content": "The spiral advancement skin flap procedure for stomal revision is a simple technique when the size of the skin defect must be reduced. The technique is described herein.", "contents": "Spiral advancement skin flap for stomal revision. The spiral advancement skin flap procedure for stomal revision is a simple technique when the size of the skin defect must be reduced. The technique is described herein."}
{"id": "PMID:1095783", "title": "Semen cultures in the diagnosis of bacterial prostatitis.", "content": "The bacterial flora of semen from normal men has been studied. Positive cultures were obtained in 45 per cent of these individuals but only in smally colony counts of non-pathogenic bacteria. The seminal flora of patients with bacterial prostatitis was then studied. Excellent correlations have been noted between cultures of prostatic secretions and semen. Semen cultures will almost invariably be diagnostic in patients with bacterial prostatis. This technique has definite usefulness in selected patients in whom it is otherwise difficult to establish a diagnosis of bacterial prostatis.", "contents": "Semen cultures in the diagnosis of bacterial prostatitis. The bacterial flora of semen from normal men has been studied. Positive cultures were obtained in 45 per cent of these individuals but only in smally colony counts of non-pathogenic bacteria. The seminal flora of patients with bacterial prostatitis was then studied. Excellent correlations have been noted between cultures of prostatic secretions and semen. Semen cultures will almost invariably be diagnostic in patients with bacterial prostatis. This technique has definite usefulness in selected patients in whom it is otherwise difficult to establish a diagnosis of bacterial prostatis."}
{"id": "PMID:1095784", "title": "Emphysematous pyelonephritis: report of 3 cases treated by nephrectomy.", "content": "Three cases of emphysematous pyelonephritis are presented. All 3 patients were diabetics, they all had Escherichia coli and there was evidence of ureteral obstruction in 1 case only. All 3 patients underwent nephrectomy and survived. Initial evaluation should be performed to rule out ureteral obstruction. Treatment should consist of a brief trial of high dose antibiotic therapy with serious consideration of nephrectomy in the face of persistent symptoms or gas. Incision and drainage are reserved for poor surgical risk patients. All patients should remain on antibacterial therapy and have frequent followup examinations.", "contents": "Emphysematous pyelonephritis: report of 3 cases treated by nephrectomy. Three cases of emphysematous pyelonephritis are presented. All 3 patients were diabetics, they all had Escherichia coli and there was evidence of ureteral obstruction in 1 case only. All 3 patients underwent nephrectomy and survived. Initial evaluation should be performed to rule out ureteral obstruction. Treatment should consist of a brief trial of high dose antibiotic therapy with serious consideration of nephrectomy in the face of persistent symptoms or gas. Incision and drainage are reserved for poor surgical risk patients. All patients should remain on antibacterial therapy and have frequent followup examinations."}
{"id": "PMID:1095785", "title": "Cine-fluoroscopic studies of ureteral function in the human renal transplant.", "content": "During a 14-month period 73 satisfactory fluoroscopic studies were done on 59 kidney transplant recipients. Ureteral peristalsis was seen in 90 per cent of these studies and there was no difference between patients receiving living related donor kidneys and those receiving cadaveric kidneys. When ureteral activity was studied during rejection episodes only 54 per cent of the studies showed activity. An effort was made to relate aperistaltic ureteral activity to renal function, warm ischemia time, urinary tract infection, number of rejection episodes and tissue matching but no positive correlation could be made except the concurrent clinical diagnosis of rejection.", "contents": "Cine-fluoroscopic studies of ureteral function in the human renal transplant. During a 14-month period 73 satisfactory fluoroscopic studies were done on 59 kidney transplant recipients. Ureteral peristalsis was seen in 90 per cent of these studies and there was no difference between patients receiving living related donor kidneys and those receiving cadaveric kidneys. When ureteral activity was studied during rejection episodes only 54 per cent of the studies showed activity. An effort was made to relate aperistaltic ureteral activity to renal function, warm ischemia time, urinary tract infection, number of rejection episodes and tissue matching but no positive correlation could be made except the concurrent clinical diagnosis of rejection."}
{"id": "PMID:1095786", "title": "The surgical approach to Peyronie's disease.", "content": "Seven patients with Peyronie's disease were treated by surgical exposure of the fibrous plaque and dermo-jet injection of steroids into the plaque under direct vision. Relief of pain was obtained in 2 of 3 patients and curvature was only moderately improved i 2 of 7 patients. One of 4 patients who were unable to perform coitus because of the curvature noted improvement after the injections. The low rate of response in this small series of patients would suggest that there is no additional benefit in the use of the dermo-jet over percutaneous injection of the plaques. The latter procedure can be performed more easily on a repeated basis over a longer period. Two patients whose plaques were excised and repalced by a dermal inlay graft have had a good response to treatment and it would appear that this technique is useful for the patient who has failed to respond to conservative measures and who is significantly disabled by the disease.", "contents": "The surgical approach to Peyronie's disease. Seven patients with Peyronie's disease were treated by surgical exposure of the fibrous plaque and dermo-jet injection of steroids into the plaque under direct vision. Relief of pain was obtained in 2 of 3 patients and curvature was only moderately improved i 2 of 7 patients. One of 4 patients who were unable to perform coitus because of the curvature noted improvement after the injections. The low rate of response in this small series of patients would suggest that there is no additional benefit in the use of the dermo-jet over percutaneous injection of the plaques. The latter procedure can be performed more easily on a repeated basis over a longer period. Two patients whose plaques were excised and repalced by a dermal inlay graft have had a good response to treatment and it would appear that this technique is useful for the patient who has failed to respond to conservative measures and who is significantly disabled by the disease."}
{"id": "PMID:1095787", "title": "A modification of the Young procedure for radical perineal prostatectomy.", "content": "A modification of the Young procedure for total perineal prostatectomy is described herein. The modification consists of an anterior and posterior paraurethral mattress suture that approximates the distal urethra and the bladder neck without constricting or interfering with the function of the sphincter muscle. None of the 55 patients with early prostatic carcinoma who have undergone this procedure has had incontinence.", "contents": "A modification of the Young procedure for radical perineal prostatectomy. A modification of the Young procedure for total perineal prostatectomy is described herein. The modification consists of an anterior and posterior paraurethral mattress suture that approximates the distal urethra and the bladder neck without constricting or interfering with the function of the sphincter muscle. None of the 55 patients with early prostatic carcinoma who have undergone this procedure has had incontinence."}
{"id": "PMID:1095788", "title": "Nephrocalcinosis in infancy and childhood.", "content": "The diangosis, clinical manifestations and management of 11 children with nephrocalcinosis encountered in a 20-year period are presented. Renal tubular acidosis, primary hyperoxaluria, primary hyperparathyroidism, exogenous hyperadrenocorticism and idiopathic hypercalcemia of infancy were the principal causes of nephrocalcinosis in this series. In the presence of normal or near-normal renal function, a 55 per cent or better cure rate can be expected. Children with significantly diminished renal function have a poor prognosis and should be considered for renal transplantation.", "contents": "Nephrocalcinosis in infancy and childhood. The diangosis, clinical manifestations and management of 11 children with nephrocalcinosis encountered in a 20-year period are presented. Renal tubular acidosis, primary hyperoxaluria, primary hyperparathyroidism, exogenous hyperadrenocorticism and idiopathic hypercalcemia of infancy were the principal causes of nephrocalcinosis in this series. In the presence of normal or near-normal renal function, a 55 per cent or better cure rate can be expected. Children with significantly diminished renal function have a poor prognosis and should be considered for renal transplantation."}
{"id": "PMID:1095789", "title": "Hamartoma and renal transplant implications.", "content": "A 19-year-old woman with tuberous sclerosis, bilateral renal hamartomas and polycystic kidneys required separate nephrectomies for severe hemorrhages 6 months apart. The case is presented, the diagnoses of the disease in relation to a negative family history are considered and the importance of early diagnosis in a potential transplant recipient and the use of leukocyte-free transufusions are discussed.", "contents": "Hamartoma and renal transplant implications. A 19-year-old woman with tuberous sclerosis, bilateral renal hamartomas and polycystic kidneys required separate nephrectomies for severe hemorrhages 6 months apart. The case is presented, the diagnoses of the disease in relation to a negative family history are considered and the importance of early diagnosis in a potential transplant recipient and the use of leukocyte-free transufusions are discussed."}
{"id": "PMID:1095793", "title": "A rabies epidemic in recently captured skunks.", "content": "An epidemic of rabies occurred within a colony of captive skunks (Mephitis mephitis). One of the animals has been infected with the virus in the wild and developed clinical illness near 7 weeks after capture. She transmitted the virus to three of her five offspring and one other adult. The disease spread to additional skunks when orphaned infants were adopted by lactating females. Although the animals were in close contact with each other, the epidemic spread slowly. Furious rabies generally did not occur and frequently rabid skunks were found dead without any clinical signs of the disease having been observed.", "contents": "A rabies epidemic in recently captured skunks. An epidemic of rabies occurred within a colony of captive skunks (Mephitis mephitis). One of the animals has been infected with the virus in the wild and developed clinical illness near 7 weeks after capture. She transmitted the virus to three of her five offspring and one other adult. The disease spread to additional skunks when orphaned infants were adopted by lactating females. Although the animals were in close contact with each other, the epidemic spread slowly. Furious rabies generally did not occur and frequently rabid skunks were found dead without any clinical signs of the disease having been observed."}
{"id": "PMID:1095794", "title": "Ibuprofen or aspirin in rheumatoid arthritis therapy.", "content": "Ibuprofen is a nonsteroidal drug with analgesic, antipyretic, and anti-inflammatory properties that was recently introduced for use in antiarthritis therapy in the United States. In a year-long double-blind multiclinic trial in 885 patients with rheumatoid arthritis, ibuprofen was at least as satisfactory as aspirin, considering both efficacy and tolerance. In the majority of patients, daily doses ranged from 800 to 1,600 mg of ibuprofen and 3 to 6 gm of aspirin. The drugs did not differ greatly in providing relief from arthritis symptoms, but ibuprofen was definitely better tolerated, especially in regard to gastrointestinal complaints. Seven percent of the ibuprofen group dropped out of the study because of adverse reactions, as compared with 16% of the aspirin group; 17% of the ibuprofen group and 31% of the aspirin group had gastrointestinal symptoms.", "contents": "Ibuprofen or aspirin in rheumatoid arthritis therapy. Ibuprofen is a nonsteroidal drug with analgesic, antipyretic, and anti-inflammatory properties that was recently introduced for use in antiarthritis therapy in the United States. In a year-long double-blind multiclinic trial in 885 patients with rheumatoid arthritis, ibuprofen was at least as satisfactory as aspirin, considering both efficacy and tolerance. In the majority of patients, daily doses ranged from 800 to 1,600 mg of ibuprofen and 3 to 6 gm of aspirin. The drugs did not differ greatly in providing relief from arthritis symptoms, but ibuprofen was definitely better tolerated, especially in regard to gastrointestinal complaints. Seven percent of the ibuprofen group dropped out of the study because of adverse reactions, as compared with 16% of the aspirin group; 17% of the ibuprofen group and 31% of the aspirin group had gastrointestinal symptoms."}
{"id": "PMID:1095805", "title": "[Differential diagnosis of acute myocardial infarct and ECG interpretation with the aid of long-distance computer inquiry].", "content": "Data are presented on the differential diagnosis of some forms of the ischaemic heart disease, myocardial infarction including, performed with the help of a long--distance enquiry of the \"Nairi-K\" computer. This is one of the adjunct methods of early and timely diagnosis of myocardial infarction that permits to differentiate it from angina pectoris, postinfarction cardiosclerosis, acute abdominal pathology, pleuropneumonia, and to determine the localization of the necrosis. The exactness of the diagnosis ranges from 93 to 95%. An information system was developed that permits a more objective assessment of the nature of the ECG changes.", "contents": "[Differential diagnosis of acute myocardial infarct and ECG interpretation with the aid of long-distance computer inquiry]. Data are presented on the differential diagnosis of some forms of the ischaemic heart disease, myocardial infarction including, performed with the help of a long--distance enquiry of the \"Nairi-K\" computer. This is one of the adjunct methods of early and timely diagnosis of myocardial infarction that permits to differentiate it from angina pectoris, postinfarction cardiosclerosis, acute abdominal pathology, pleuropneumonia, and to determine the localization of the necrosis. The exactness of the diagnosis ranges from 93 to 95%. An information system was developed that permits a more objective assessment of the nature of the ECG changes."}
{"id": "PMID:1095816", "title": "[Covering of fistulae after operations of glaucoma (author's transl)].", "content": "It is recommended to use a certain method to cover cystic oozing cushions after filtering operations of glaucoma. To this effect a lamellar scleral flap is formed which is laid over the previously exposed scleral fissure. An impediment of the filtration of aqueous humour is avoided by means of suitable fixation of Tenon's capsule.", "contents": "[Covering of fistulae after operations of glaucoma (author's transl)]. It is recommended to use a certain method to cover cystic oozing cushions after filtering operations of glaucoma. To this effect a lamellar scleral flap is formed which is laid over the previously exposed scleral fissure. An impediment of the filtration of aqueous humour is avoided by means of suitable fixation of Tenon's capsule."}
{"id": "PMID:1095817", "title": "[Correction of Blepharochalasis (author's transl)].", "content": "Blepharochalasis implies the symptom or general term (Kettesy) applied to the slackening and thinning out of the upper lid. From a clinical point of view and in a wide sense this includes Cutix laxa senilis or senile epiblepharon. The following surgical procedures restore the lost contract between the skin and the lid elevator. 1. Upper plapebral furrow formation sutures (Kettesy). 2. Blepharochalasis surgery according to the method of Imre. This is a \"mild ptosis operation\" which is indicated in advanced slackening and dehiszenz of the Fascia tarso orbitalis. 3. The \"controlled skin resection\" which permits a testing of the function before the skin resection, prevents the excision of muscle fibres as well as too large and irregular skin stripes and provides for a minimum haemorrhage. For this purpose the skin fold near to the upper tarsal margin is lifted up and marked with running sutures along the line of expected upper palpebral furrow. After resection of the lifted lid skin the scarcely bleeding wound is closed with running sutures.", "contents": "[Correction of Blepharochalasis (author's transl)]. Blepharochalasis implies the symptom or general term (Kettesy) applied to the slackening and thinning out of the upper lid. From a clinical point of view and in a wide sense this includes Cutix laxa senilis or senile epiblepharon. The following surgical procedures restore the lost contract between the skin and the lid elevator. 1. Upper plapebral furrow formation sutures (Kettesy). 2. Blepharochalasis surgery according to the method of Imre. This is a \"mild ptosis operation\" which is indicated in advanced slackening and dehiszenz of the Fascia tarso orbitalis. 3. The \"controlled skin resection\" which permits a testing of the function before the skin resection, prevents the excision of muscle fibres as well as too large and irregular skin stripes and provides for a minimum haemorrhage. For this purpose the skin fold near to the upper tarsal margin is lifted up and marked with running sutures along the line of expected upper palpebral furrow. After resection of the lifted lid skin the scarcely bleeding wound is closed with running sutures."}
{"id": "PMID:1095819", "title": "Self-mutilation in mice associated with otitis media.", "content": "Mutilation of the external ear and adjacent tissues was an enzootic problem in outbred Mcr:(ICR) breeder mice in a commercial colony. Necropsy examination of affected and randomly selected, nonaffected adult breeder mice revealed a probable association of the cutaneous lesions with suppurative otitis media. Several microorganisms, including Mycoplasma pulmonis and Pasteurella pneumotropica, were isolated from the tympanic bullae.", "contents": "Self-mutilation in mice associated with otitis media. Mutilation of the external ear and adjacent tissues was an enzootic problem in outbred Mcr:(ICR) breeder mice in a commercial colony. Necropsy examination of affected and randomly selected, nonaffected adult breeder mice revealed a probable association of the cutaneous lesions with suppurative otitis media. Several microorganisms, including Mycoplasma pulmonis and Pasteurella pneumotropica, were isolated from the tympanic bullae."}
{"id": "PMID:1095822", "title": "Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections.", "content": "An immunohistochemical staining to demonstrate the hepatitis B core and surface antigens (HBc&sAg) on formalin-fixed, paraffin-embedded liver tissue was investigated. The staining is believed to be valid for the reasons that: (1) HBc&s AG-positive and control tissues were properly selected. (2) The immunospecificities of the antisera to HBc Ag or HBs Ag were verified by immunoelectron microscopy and by conducting the neutralization controls in the staining procedure. (3) The staining results were identical with those shown on unfixed frozen sections from the same cases. The immunoreactive sites of HBc&s Ag are shown to be preserved in the formalin-fixed, paraffin-embedded tissue, and they can be demonstrated by either direct or indirect immunofluorescent and immunoperoxidase methods. The pronase digestion of the tissue sections quenches the nonspecific background staining and thus facilitates the microscopic examination. The indirect immunofluorescent method is more sensitive than the direct. The localization of HBs Ag in liver cells shown by immunohistochemical method is identical with that demonstrated by orcein histochemical stain. The described method demonstrating the HBc Ag in paraffin tissue may offer an additional means in making a specific etiologic diagnosis of hepatitis B viral disease.", "contents": "Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections. An immunohistochemical staining to demonstrate the hepatitis B core and surface antigens (HBc&sAg) on formalin-fixed, paraffin-embedded liver tissue was investigated. The staining is believed to be valid for the reasons that: (1) HBc&s AG-positive and control tissues were properly selected. (2) The immunospecificities of the antisera to HBc Ag or HBs Ag were verified by immunoelectron microscopy and by conducting the neutralization controls in the staining procedure. (3) The staining results were identical with those shown on unfixed frozen sections from the same cases. The immunoreactive sites of HBc&s Ag are shown to be preserved in the formalin-fixed, paraffin-embedded tissue, and they can be demonstrated by either direct or indirect immunofluorescent and immunoperoxidase methods. The pronase digestion of the tissue sections quenches the nonspecific background staining and thus facilitates the microscopic examination. The indirect immunofluorescent method is more sensitive than the direct. The localization of HBs Ag in liver cells shown by immunohistochemical method is identical with that demonstrated by orcein histochemical stain. The described method demonstrating the HBc Ag in paraffin tissue may offer an additional means in making a specific etiologic diagnosis of hepatitis B viral disease."}
{"id": "PMID:1095838", "title": "Pharmacological and ultrastructural maturation of serotonergic synapses during ontogeny.", "content": "Studies were made on the course of maturation of serotonergic synapses during ontogeny in rat brain. Mature synaptosomes containing the same five types of synatic vesicles as in the adult, including small dense core vesicles, could be isolated in low proportion from the brain of 1-day-old rats. Although the buoyant density of these synaptosomes varied more than in the adult, the 5-hydroxytryptamine (5-HT) synaptosomes at the two age groups had similar sedimentation characteristics. Ouabain and imipramine, which block active transport of 5-HT, and reserpine, which blocks its granular storage, resulted respectively in a similar slight inhibition of uptake and accumulation of (-14C)5-HT in synaptosomes of 19-day foetuses. Transport and storage of (-14C)5-HT in the brain stem matured simultaneously with endogenous 5-HT content. In subcellular fractionation of the brain 5-HT content, the percentage of 5-HT in the supernatant was significantly lower in neonatal than in adult rats. After treatments with monoamine oxidase inhibitors (MAOIs) and reserpine plus MAOI, respectively, the maximum brain content and subcellular distribution of 5-HT were similar in 1-day-old and adult rats; the result suggests the existence of some other binding mechanism besides the Mg++-ATP-dependent granular storage. Imipramine and N,N-dimethyltryptamine, which cause stimulation of 5-HT receptors, decreased the turnover of brain 5-HT by 40% in adults but had no effect in neonatal rats. Immobilization increased the turnover of brain 5-HT by 35% in adults but had no effect in neonatal rats, whereas fasting increased it by 20% in adults and by 150% in neonatal rats. At 3 weeks of age the responses to imipramine, immobilization and fasting resembled those seen in adults. These responses occurred later than the appearance of the endogenous content, transport and storage of 5-HT and may require maturation of synaptic junctions, the latest neuronal structures to develop.", "contents": "Pharmacological and ultrastructural maturation of serotonergic synapses during ontogeny. Studies were made on the course of maturation of serotonergic synapses during ontogeny in rat brain. Mature synaptosomes containing the same five types of synatic vesicles as in the adult, including small dense core vesicles, could be isolated in low proportion from the brain of 1-day-old rats. Although the buoyant density of these synaptosomes varied more than in the adult, the 5-hydroxytryptamine (5-HT) synaptosomes at the two age groups had similar sedimentation characteristics. Ouabain and imipramine, which block active transport of 5-HT, and reserpine, which blocks its granular storage, resulted respectively in a similar slight inhibition of uptake and accumulation of (-14C)5-HT in synaptosomes of 19-day foetuses. Transport and storage of (-14C)5-HT in the brain stem matured simultaneously with endogenous 5-HT content. In subcellular fractionation of the brain 5-HT content, the percentage of 5-HT in the supernatant was significantly lower in neonatal than in adult rats. After treatments with monoamine oxidase inhibitors (MAOIs) and reserpine plus MAOI, respectively, the maximum brain content and subcellular distribution of 5-HT were similar in 1-day-old and adult rats; the result suggests the existence of some other binding mechanism besides the Mg++-ATP-dependent granular storage. Imipramine and N,N-dimethyltryptamine, which cause stimulation of 5-HT receptors, decreased the turnover of brain 5-HT by 40% in adults but had no effect in neonatal rats. Immobilization increased the turnover of brain 5-HT by 35% in adults but had no effect in neonatal rats, whereas fasting increased it by 20% in adults and by 150% in neonatal rats. At 3 weeks of age the responses to imipramine, immobilization and fasting resembled those seen in adults. These responses occurred later than the appearance of the endogenous content, transport and storage of 5-HT and may require maturation of synaptic junctions, the latest neuronal structures to develop."}
{"id": "PMID:1095839", "title": "Copayments for ambulatory care: penny-wise and pound-foolish.", "content": "The California \"copayment experiment\" imposed a charge of $1 on certain Medicaid beneficiaries for the first two visits to a doctor and 50 cents for the first two drug prescriptions each month, effective January 1, 1972. Data on utilization rates were gathered for six months before this date and for 12 months after it. While other administrative requirements, like prior authorization of certain services, doubtless also played a part, it was found that, following the start of copayment, utilization of ambulatory doctor's office visits and other services associated with them showed a decline, relative to that of the non-copayment cohort. After a brief lag, however, hospitalization rates in the copay cohort rose to levels higher than those of the non-copayment cohort-more than offsetting the savings to the state from the reduction of ambulatory service use rates. Due presumably to the neglect of early medical care because of the inhibiting effect of the copayments, these higher use rates of costly hospitalizations suggest that financial deterrents on access to ambulatory service by poor people are penny-wise and pound-foolish, not to mention their effects on health and well-being.", "contents": "Copayments for ambulatory care: penny-wise and pound-foolish. The California \"copayment experiment\" imposed a charge of $1 on certain Medicaid beneficiaries for the first two visits to a doctor and 50 cents for the first two drug prescriptions each month, effective January 1, 1972. Data on utilization rates were gathered for six months before this date and for 12 months after it. While other administrative requirements, like prior authorization of certain services, doubtless also played a part, it was found that, following the start of copayment, utilization of ambulatory doctor's office visits and other services associated with them showed a decline, relative to that of the non-copayment cohort. After a brief lag, however, hospitalization rates in the copay cohort rose to levels higher than those of the non-copayment cohort-more than offsetting the savings to the state from the reduction of ambulatory service use rates. Due presumably to the neglect of early medical care because of the inhibiting effect of the copayments, these higher use rates of costly hospitalizations suggest that financial deterrents on access to ambulatory service by poor people are penny-wise and pound-foolish, not to mention their effects on health and well-being."}
{"id": "PMID:1095840", "title": "Emphasizing \"level of care\" over \"length of stay\" in hospital utilization review.", "content": "Utilization review has heretofore emphasized \"length of stay\" as the criterion for deciding when the patient is ready to leave the hospital. We suggest that \"level of care\" (the type, number, and/or intensity of a combination of physician, skilled nursing, and ancillary services) received by the patient should replace length of stay as the major \"decision\" criterion for deciding on continued hospitalization: \"appropriate hospital placement\" means the patient is currently receiving a level of care available only in the hospital. Length of stay should be the criterion for signaling intervals when patients are (re) reviewed to determine the currently needed level of care. Explicit criteria for determining when a patient is receiving a hospital level of care should be developed. Explicit criteria will increase objectivity, provide guidance to utilization review coordinators, and assist the hospital in defending its utilization review decisions to an intermediary or Professional Standards Review Organization (PSRO). Level of care will be reflected in a patient's \"service profile,\" a listing of all the services being received by the patient on any given day of hospitalization. The services profile varies throughout hospitalization; hospitalization is appropriate when a patient's current services profile constitutes a level of care available only in the hospital.", "contents": "Emphasizing \"level of care\" over \"length of stay\" in hospital utilization review. Utilization review has heretofore emphasized \"length of stay\" as the criterion for deciding when the patient is ready to leave the hospital. We suggest that \"level of care\" (the type, number, and/or intensity of a combination of physician, skilled nursing, and ancillary services) received by the patient should replace length of stay as the major \"decision\" criterion for deciding on continued hospitalization: \"appropriate hospital placement\" means the patient is currently receiving a level of care available only in the hospital. Length of stay should be the criterion for signaling intervals when patients are (re) reviewed to determine the currently needed level of care. Explicit criteria for determining when a patient is receiving a hospital level of care should be developed. Explicit criteria will increase objectivity, provide guidance to utilization review coordinators, and assist the hospital in defending its utilization review decisions to an intermediary or Professional Standards Review Organization (PSRO). Level of care will be reflected in a patient's \"service profile,\" a listing of all the services being received by the patient on any given day of hospitalization. The services profile varies throughout hospitalization; hospitalization is appropriate when a patient's current services profile constitutes a level of care available only in the hospital."}
{"id": "PMID:1095841", "title": "Optometric prescribing patterns: a quality comparison of Medicaid and non-Medicaid practitioners.", "content": "This study analyzes five variables related to quality and utilization of optometric prescribing of eyeglasses. The practices of optometrists participating in the New York City Medicaid Program were compared with those not participating. The variables chosen for this study can be analyzed by computer and seem to have wide applicability for monitoring of optometry services. Significant differences were found in four of the five variables under review. Overall, non-Medicaid patients appear to be receiving higher quality optometric care and less unnecessary care. Many of these differences, however, depend on the setting of the practice-whether solo, group, or corporate. Of additional note, wide variations occurred in the patterns of practice for non-Medicaid, as well as Medicaid, optometrists.", "contents": "Optometric prescribing patterns: a quality comparison of Medicaid and non-Medicaid practitioners. This study analyzes five variables related to quality and utilization of optometric prescribing of eyeglasses. The practices of optometrists participating in the New York City Medicaid Program were compared with those not participating. The variables chosen for this study can be analyzed by computer and seem to have wide applicability for monitoring of optometry services. Significant differences were found in four of the five variables under review. Overall, non-Medicaid patients appear to be receiving higher quality optometric care and less unnecessary care. Many of these differences, however, depend on the setting of the practice-whether solo, group, or corporate. Of additional note, wide variations occurred in the patterns of practice for non-Medicaid, as well as Medicaid, optometrists."}
{"id": "PMID:1095842", "title": "Medicine's great schism: prevention vs. cure: an historical interpretation.", "content": "All societies, primitive and advanced, demand therapeutic services, but a society must develop sophisticated prerequisites before it can support preventive services. This discordance in the origins of curative medicine and public health does not explain why a schism between them still persists. This gap should be closed since medicine has cured most curable diseases and the residue of chronic conditions is best handled by preventing them or detecting them before they become medical crises. Reasons for persistence of the schism include; early concentration of public health on environmental sanitation to the exclusion of medicine; identification of public health was governmental bureaucracy; linking care of the poor with tight-fisted welfarism; existence of two separate educational systems, the view of doctors, who are activists by nature, toward the excitement of cure; the custom of paying only for active therapy; and the orientation of the Hippocratic ethic toward individuals.", "contents": "Medicine's great schism: prevention vs. cure: an historical interpretation. All societies, primitive and advanced, demand therapeutic services, but a society must develop sophisticated prerequisites before it can support preventive services. This discordance in the origins of curative medicine and public health does not explain why a schism between them still persists. This gap should be closed since medicine has cured most curable diseases and the residue of chronic conditions is best handled by preventing them or detecting them before they become medical crises. Reasons for persistence of the schism include; early concentration of public health on environmental sanitation to the exclusion of medicine; identification of public health was governmental bureaucracy; linking care of the poor with tight-fisted welfarism; existence of two separate educational systems, the view of doctors, who are activists by nature, toward the excitement of cure; the custom of paying only for active therapy; and the orientation of the Hippocratic ethic toward individuals."}
{"id": "PMID:1095843", "title": "Cost-sharing and prior authorization effects on Medicaid services in California: part I. The beneficiaries' reactions.", "content": "In January 1972, California initiated a cost-sharing \"experiment\" as a means of containing rising costs of the Medicaid program. Beneficiaries who had some personal assets were required to pay a token amount for each doctor visit or prescription. This was expected to deter unnecessary services without affecting really needed care. An interview survey was made of beneficiaries continuously in copayment status throughout the 18 months of the experiment. Most beneficiaries had poor knowledge of the program, and tended to confuse copayment with the concurrent constraint of prior authorization for certain classes of service. The beneficiaries reported that the copayment was almost always collected, and most thought it had not affected their health care, but a significant 17 per cent throught it had reduced the care available to them, and these 17 per cent were for the most part in households with high medical need.", "contents": "Cost-sharing and prior authorization effects on Medicaid services in California: part I. The beneficiaries' reactions. In January 1972, California initiated a cost-sharing \"experiment\" as a means of containing rising costs of the Medicaid program. Beneficiaries who had some personal assets were required to pay a token amount for each doctor visit or prescription. This was expected to deter unnecessary services without affecting really needed care. An interview survey was made of beneficiaries continuously in copayment status throughout the 18 months of the experiment. Most beneficiaries had poor knowledge of the program, and tended to confuse copayment with the concurrent constraint of prior authorization for certain classes of service. The beneficiaries reported that the copayment was almost always collected, and most thought it had not affected their health care, but a significant 17 per cent throught it had reduced the care available to them, and these 17 per cent were for the most part in households with high medical need."}
{"id": "PMID:1095872", "title": "Kinetics of tumor growth and of cell proliferation in U.R.D.T. cancers: therapeutic implications.", "content": "After a brief survey of the factors of clinical tumor radioresistance the available data on the growth rate and on the kinetics of cell proliferation of U.R.D.T. tumors are reviewed.  The growth fraction and the cell turnover rate appear to be similar in U.R.D.T. tumors and in other squamous cell carcinomas.  These two parameters are correlated with clinical tumor radiosensitivity and chemosensitivity.  The possible value of tailoring fractionation and drug administration to the biological characteristics of the tumor is discussed.", "contents": "Kinetics of tumor growth and of cell proliferation in U.R.D.T. cancers: therapeutic implications. After a brief survey of the factors of clinical tumor radioresistance the available data on the growth rate and on the kinetics of cell proliferation of U.R.D.T. tumors are reviewed.  The growth fraction and the cell turnover rate appear to be similar in U.R.D.T. tumors and in other squamous cell carcinomas.  These two parameters are correlated with clinical tumor radiosensitivity and chemosensitivity.  The possible value of tailoring fractionation and drug administration to the biological characteristics of the tumor is discussed."}
{"id": "PMID:1095873", "title": "Acalculous parotid sialadenopathy.", "content": "Acalculous inflammation of the parotid gland has been regarded by some as a specific disorder that can be staged as to the severity of the disease process.  A review of the literature, however, indicates a divergence of opinion.  It is the purpose of this study to test this hypothesis by a retrospective double-blind analysis of clinical, sialographic and histopathologic findings.  Forty-two patients who were seen by the Department of Otolaryngology, Washington University School of Medicine from 1961 to 1970 were evaluated by two separate teams.  There were 20 sialograms and 30 pathologic specimens, and all were re-examined.  Eleven patients had both sialographic studies and histopathologic examination.  The clinical presentations and sialographic findings were reviewed.  These findings were then compared to each other.  Our results indicate that we could not evaluate the severity of the clinical disease by sialography or histopathology.  There was no consistent method of staging the sialographic and pathologic findings.  In addition, there was no correlation between these two parameters.  They reflect neither the patient's clinical syndrome pattern nor the extent of disease.  It appears that the parotid gland responds variable to inflammation.  These observations are exemplified by case histories.", "contents": "Acalculous parotid sialadenopathy. Acalculous inflammation of the parotid gland has been regarded by some as a specific disorder that can be staged as to the severity of the disease process.  A review of the literature, however, indicates a divergence of opinion.  It is the purpose of this study to test this hypothesis by a retrospective double-blind analysis of clinical, sialographic and histopathologic findings.  Forty-two patients who were seen by the Department of Otolaryngology, Washington University School of Medicine from 1961 to 1970 were evaluated by two separate teams.  There were 20 sialograms and 30 pathologic specimens, and all were re-examined.  Eleven patients had both sialographic studies and histopathologic examination.  The clinical presentations and sialographic findings were reviewed.  These findings were then compared to each other.  Our results indicate that we could not evaluate the severity of the clinical disease by sialography or histopathology.  There was no consistent method of staging the sialographic and pathologic findings.  In addition, there was no correlation between these two parameters.  They reflect neither the patient's clinical syndrome pattern nor the extent of disease.  It appears that the parotid gland responds variable to inflammation.  These observations are exemplified by case histories."}
{"id": "PMID:1095875", "title": "Regional skin flaps in partial laryngectomy.", "content": "The use of regional neck flaps for rehabilitation has permitted an extension of operations on the glottis.  The resection may include both vocal cords, both ventricular bands, one arytenoid and slight subglottic extension in a one- or two-stage technique.  The resultant voice is low pitched, soft and has limited range.  The cure rate was 85 percent.", "contents": "Regional skin flaps in partial laryngectomy. The use of regional neck flaps for rehabilitation has permitted an extension of operations on the glottis.  The resection may include both vocal cords, both ventricular bands, one arytenoid and slight subglottic extension in a one- or two-stage technique.  The resultant voice is low pitched, soft and has limited range.  The cure rate was 85 percent."}
{"id": "PMID:1095879", "title": "Steroids and steroid metabolism in plant tissue cultures.", "content": "The steroids which have been isolated from plant callus and suspension cultures are reviewed. In addition, the research involving the use of plant tissue cultures to investigate steroid biosynthesis and metabolism is summarized.", "contents": "Steroids and steroid metabolism in plant tissue cultures. The steroids which have been isolated from plant callus and suspension cultures are reviewed. In addition, the research involving the use of plant tissue cultures to investigate steroid biosynthesis and metabolism is summarized."}
{"id": "PMID:1095889", "title": "Beh\u00e7et's disease. Report of 41 cases and a review of the literature.", "content": "Several important conclusions may be derived on the basis of our experience with Behcet's disease (B.D.) and a review of the literature, namely: 1. B.D. is a systemic disease characterized by exacerbations and remissions of unpredictable duration, which affects mainly males between the ages of 20 to 30 years. Although its incidence is higher in the eastern hemisphere cases presented in all parts of the world. 2. The common histopathological lesion of all clinical manifestations appears to be a vasculitis. The etiology of B.D. is unknown. A viral etiology has been proposed but not confirmed. The presence of autoantibodies and lymphocyte sensitization to mucosal antigen has been observed but the precise significance of these findings in relation to the pathogenesis of the disease is presently undetermined. 3. The clinical manigestations present in most of the patients and considered diagnostic for B.D. are oral and genital ulcers, uveitis and skin lesions (especially erythema nodosum-like lesions or non-specific skin reactivity to needle pricks). The presence of three of the above manifestations are obligatory for the diagnosis. The other common clinical manifestations occurring in B.D. are: arthritis (44%); thrombophlebitis (24%), and various neurological syndromes (18%). Less frequent complications of the disease include: arterial thrombosis and aneurysm of both the systemic and pulmonary circulation, colitis, epididymitis and orchitis. 4. The serious chronic sequelae of this disease consist of blindness in up to 33% of patients with uveitis, vena caval obstruction and paralysis of limbs. Mortality, usually caused by meningoencephalitis, is rare. 5. The patients may present to different medical specialities, e.g., dermatology, gynecology, ophthalmology, neurology and internal medicine. To facilitate the correct diagnosis the essential criteria should be actively sought as they are not always the cause for medical consultation. 6. The diagnosis of B.D. is based only on clinical grounds as there are no pathognomonic laboratory or histopathological features. 7. Evaluation of treatment in B.D. is difficult, because of the naturally unpredictable course of the disease. Many drugs have been tried in the treatment of B.D. including antibiotics, anti-inflammatory drugs and corticosteroids, all with equivocal effects. Recently several additional treatment schedules have been suggested. Immunosuppressive drugs have been shown to have some beneficial effect, especially on uveitis. Fresh blood or plasma transfusions may prolong remissions. 8. The finding of decreased plasma fibrinolytic activity in patients with active B.D. represents the first observation of a possible disturbance in the blood coagulation mechanism and suggests that the use of fibrinolytic agents may be rewarding.", "contents": "Beh\u00e7et's disease. Report of 41 cases and a review of the literature. Several important conclusions may be derived on the basis of our experience with Behcet's disease (B.D.) and a review of the literature, namely: 1. B.D. is a systemic disease characterized by exacerbations and remissions of unpredictable duration, which affects mainly males between the ages of 20 to 30 years. Although its incidence is higher in the eastern hemisphere cases presented in all parts of the world. 2. The common histopathological lesion of all clinical manifestations appears to be a vasculitis. The etiology of B.D. is unknown. A viral etiology has been proposed but not confirmed. The presence of autoantibodies and lymphocyte sensitization to mucosal antigen has been observed but the precise significance of these findings in relation to the pathogenesis of the disease is presently undetermined. 3. The clinical manigestations present in most of the patients and considered diagnostic for B.D. are oral and genital ulcers, uveitis and skin lesions (especially erythema nodosum-like lesions or non-specific skin reactivity to needle pricks). The presence of three of the above manifestations are obligatory for the diagnosis. The other common clinical manifestations occurring in B.D. are: arthritis (44%); thrombophlebitis (24%), and various neurological syndromes (18%). Less frequent complications of the disease include: arterial thrombosis and aneurysm of both the systemic and pulmonary circulation, colitis, epididymitis and orchitis. 4. The serious chronic sequelae of this disease consist of blindness in up to 33% of patients with uveitis, vena caval obstruction and paralysis of limbs. Mortality, usually caused by meningoencephalitis, is rare. 5. The patients may present to different medical specialities, e.g., dermatology, gynecology, ophthalmology, neurology and internal medicine. To facilitate the correct diagnosis the essential criteria should be actively sought as they are not always the cause for medical consultation. 6. The diagnosis of B.D. is based only on clinical grounds as there are no pathognomonic laboratory or histopathological features. 7. Evaluation of treatment in B.D. is difficult, because of the naturally unpredictable course of the disease. Many drugs have been tried in the treatment of B.D. including antibiotics, anti-inflammatory drugs and corticosteroids, all with equivocal effects. Recently several additional treatment schedules have been suggested. Immunosuppressive drugs have been shown to have some beneficial effect, especially on uveitis. Fresh blood or plasma transfusions may prolong remissions. 8. The finding of decreased plasma fibrinolytic activity in patients with active B.D. represents the first observation of a possible disturbance in the blood coagulation mechanism and suggests that the use of fibrinolytic agents may be rewarding."}
{"id": "PMID:1095903", "title": "[Phagocytosis of cell wall defective bacteria by human macrophages (author's transl)].", "content": "Statistically significant better phagocytosis of spheroplasts was found by comparing the rate of phagocytosis of normal and Penicillin-G induced cell wall defective bacterial (spheroplasts) by human macrophages in isotonic media using a Proteus mirabilis strain as test organism. This provides additional information concerning the minor role of cell wall defective bacteria as persisters in chronic infections.", "contents": "[Phagocytosis of cell wall defective bacteria by human macrophages (author's transl)]. Statistically significant better phagocytosis of spheroplasts was found by comparing the rate of phagocytosis of normal and Penicillin-G induced cell wall defective bacterial (spheroplasts) by human macrophages in isotonic media using a Proteus mirabilis strain as test organism. This provides additional information concerning the minor role of cell wall defective bacteria as persisters in chronic infections."}
{"id": "PMID:1095904", "title": "Bacteriophages of Nocardia asteroides.", "content": "101 Nocardia strains from different species and different sources were examined with respect to their lysogeny. 18 phages from 17 Nocardia asteroides strains could be isolated, purified, and propagated. All phages were released spontaneously. Induction experiments with mitomycin C and UV-rays brought no practicale advantages. Using 14 of the 18 phages, 38 of the 101 Nocardia strains tested could be grouped into 18 phage patterns. Nearly all of the typable strains belonged to the species Nocardia asteroides.", "contents": "Bacteriophages of Nocardia asteroides. 101 Nocardia strains from different species and different sources were examined with respect to their lysogeny. 18 phages from 17 Nocardia asteroides strains could be isolated, purified, and propagated. All phages were released spontaneously. Induction experiments with mitomycin C and UV-rays brought no practicale advantages. Using 14 of the 18 phages, 38 of the 101 Nocardia strains tested could be grouped into 18 phage patterns. Nearly all of the typable strains belonged to the species Nocardia asteroides."}
{"id": "PMID:1095905", "title": "Proteolytic activity of Mycoplasma salivarium and Mycoplasma orale 1.", "content": "Proteolytic activity was found in whole cells and cultural supernatants of Mycoplasma salivarium (PG20 and B isolates) and Mycoplasma orale 1 (CH 19299 and B isolates). Additionally, the activity was examined in cell membrane soluble fractions of PG20 and CH 19299, and detected in them. The level of the activity appeared higher in M. salivarium than M. orale 1. And some differences were found between these mycoplasmas in the affinity for substrates as a result of examination of the activity in cultural supernatants using horse serum, casein and bovine albumin as substrates. That is, PG20 had a higher affinity for horse serum proteins than casein, while CH 19299 the latter than the former, although the lowest affinity for bovine albumin was common to these two strains.", "contents": "Proteolytic activity of Mycoplasma salivarium and Mycoplasma orale 1. Proteolytic activity was found in whole cells and cultural supernatants of Mycoplasma salivarium (PG20 and B isolates) and Mycoplasma orale 1 (CH 19299 and B isolates). Additionally, the activity was examined in cell membrane soluble fractions of PG20 and CH 19299, and detected in them. The level of the activity appeared higher in M. salivarium than M. orale 1. And some differences were found between these mycoplasmas in the affinity for substrates as a result of examination of the activity in cultural supernatants using horse serum, casein and bovine albumin as substrates. That is, PG20 had a higher affinity for horse serum proteins than casein, while CH 19299 the latter than the former, although the lowest affinity for bovine albumin was common to these two strains."}
{"id": "PMID:1095911", "title": "Structural aspects of the milk clotting process. Comparative features with the blood clotting process.", "content": "The enzyme chymosin and its substrate, a casein fraction called k-casein, are involved in the milk clotting process. Recent data concerning the structure (peptide and sugar moieties) of various k-caseins and their role in casein micelles formation and stabilization are presented. The molecular events occurring during the primary phase of chymosin action on k-casein are discussed. Finally some structural features concerning more particularly the caseinoglycopeptides and the fibrinopeptides as well as the action of chymosin and thrombin involved in the milk and blood clotting processes are compared. Three examples of sequences of portions of k-caseins and fibrinogen presenting homology are presented.", "contents": "Structural aspects of the milk clotting process. Comparative features with the blood clotting process. The enzyme chymosin and its substrate, a casein fraction called k-casein, are involved in the milk clotting process. Recent data concerning the structure (peptide and sugar moieties) of various k-caseins and their role in casein micelles formation and stabilization are presented. The molecular events occurring during the primary phase of chymosin action on k-casein are discussed. Finally some structural features concerning more particularly the caseinoglycopeptides and the fibrinopeptides as well as the action of chymosin and thrombin involved in the milk and blood clotting processes are compared. Three examples of sequences of portions of k-caseins and fibrinogen presenting homology are presented."}
{"id": "PMID:1095913", "title": "Induction and mutagenesis of prophage lambda in Escherichia coli K12 by metabolites of aflatoxin B1.", "content": "Like most carcinogens, aflatoxin B1 must be activated by mammalian microsomal enzymes to give rise to coupounds active on bacteria. These compounds act as inducers of E. coli K12 (lambda) at a high efficiency, whereas unmodified aflatoxin B1 has no effect. Moreover, metabolites of aflatoxin B1 have a mutagenic action on phage lambda, as shown by the appearance of clear plaque mutants. We propose the hypothesis that the same derivative is responsible for carcinogenesis of liver cells by aflatoxin B1. Therefore, our system provides a simple way of measuring in vitro, in the same assay, the mutagenic and inducing activities of compounds to which the cells are permeable, thereby detecting potentially carcinogenic agents.", "contents": "Induction and mutagenesis of prophage lambda in Escherichia coli K12 by metabolites of aflatoxin B1. Like most carcinogens, aflatoxin B1 must be activated by mammalian microsomal enzymes to give rise to coupounds active on bacteria. These compounds act as inducers of E. coli K12 (lambda) at a high efficiency, whereas unmodified aflatoxin B1 has no effect. Moreover, metabolites of aflatoxin B1 have a mutagenic action on phage lambda, as shown by the appearance of clear plaque mutants. We propose the hypothesis that the same derivative is responsible for carcinogenesis of liver cells by aflatoxin B1. Therefore, our system provides a simple way of measuring in vitro, in the same assay, the mutagenic and inducing activities of compounds to which the cells are permeable, thereby detecting potentially carcinogenic agents."}
{"id": "PMID:1095914", "title": "An evaluation of the micronuclei test using triethylenemelamine, trimethylphosphate, hycanthone and niridazole.", "content": "To determine the feasibility of the micronuclei procedure for cytogenetic studies, a comparatively weak chromosome breaking agent, trimethylphosphate (TMP) and the potent alkylating agent, triethylenemelamine (TEM) were evaluated. The procedure followed was that of Matter and Schmid with the following modifications: (a) direct flushing of bone marrow with 0.2 ml calf fetal serum. (b) air drying slides for a period of only I h, and (c) the use of pH 6.0 phosphate buffer to dilute both Wright and Giemsa stains. With this technique a dose response curve was generated for both TMP and TEM, using mice as the experimental animal. With TMP, a doubling over background was found when a concentration of 0.5 g/kg per day for five days was administered. To establish a statistically significant doubling dose over the control, a minimum of five animals must be used with 2000 polychromatic cells being analyzed per animal. Of the two antischistosomal agents tested, hycanthone yielded an increase of 20-fold in the number of micronuclei over control at 40 mg/kg administered i.p. for five days, while with niridazole no increase in micronuclei at several concentrations tested both by single and multiple injection was found. The results obtained with these compounds compare favorably with what has been reported for the standard in vivo metaphase analysis.", "contents": "An evaluation of the micronuclei test using triethylenemelamine, trimethylphosphate, hycanthone and niridazole. To determine the feasibility of the micronuclei procedure for cytogenetic studies, a comparatively weak chromosome breaking agent, trimethylphosphate (TMP) and the potent alkylating agent, triethylenemelamine (TEM) were evaluated. The procedure followed was that of Matter and Schmid with the following modifications: (a) direct flushing of bone marrow with 0.2 ml calf fetal serum. (b) air drying slides for a period of only I h, and (c) the use of pH 6.0 phosphate buffer to dilute both Wright and Giemsa stains. With this technique a dose response curve was generated for both TMP and TEM, using mice as the experimental animal. With TMP, a doubling over background was found when a concentration of 0.5 g/kg per day for five days was administered. To establish a statistically significant doubling dose over the control, a minimum of five animals must be used with 2000 polychromatic cells being analyzed per animal. Of the two antischistosomal agents tested, hycanthone yielded an increase of 20-fold in the number of micronuclei over control at 40 mg/kg administered i.p. for five days, while with niridazole no increase in micronuclei at several concentrations tested both by single and multiple injection was found. The results obtained with these compounds compare favorably with what has been reported for the standard in vivo metaphase analysis."}
{"id": "PMID:1095918", "title": "Mutagenicity testing with Salmonella typhimurium strains. I. Unusual phenotypes of the tester strains.", "content": "Some of the Salmonella typhimurium strains in mutagenesis testing are atypical. Unlike the wild S. typhimurium these do not produce H-2-S, are not agglutinated by S. typhimurium typing sera and on fermentation of carbohydrates they do not produce gas. Thus these tester strains cannot readily be identified as S. typhimurium by standard laboratory procedures. This may cause a problem in the differentiation of tester strains from bacterial contaminants. The possibility exits that these phenotypic traits may affect the response of the strains to mutagens.", "contents": "Mutagenicity testing with Salmonella typhimurium strains. I. Unusual phenotypes of the tester strains. Some of the Salmonella typhimurium strains in mutagenesis testing are atypical. Unlike the wild S. typhimurium these do not produce H-2-S, are not agglutinated by S. typhimurium typing sera and on fermentation of carbohydrates they do not produce gas. Thus these tester strains cannot readily be identified as S. typhimurium by standard laboratory procedures. This may cause a problem in the differentiation of tester strains from bacterial contaminants. The possibility exits that these phenotypic traits may affect the response of the strains to mutagens."}
{"id": "PMID:1095919", "title": "Protein synthesis and the recovery of both survival and cytoplasmic \"petite\" mutation in ultraviolet-treated yeast cells. I. Nuclear-directed protein synthesis.", "content": "The contribution of nuclear-directed protein synthesis in the repair of lethal and mitochondrial genetic damage after UV-irradiation of exponential and stationary phage haploid yeast cells was examined. This was carried out using cycloheximide (CH), a specific inhibitor of nuclear protein synthesis. It appears that nuclear protein synthesis is required for the increase in survival seen after the liquid holding of cells at both stages, as well as for the \"petite\" recovery seen after the liquid holding of exponential phase cells. The characteristic negative liquid holding effect observed for the UV induction of \"petites\" in stationary phase cells (increase of the frequency of \"petites\" during storage) remained following all the treatments which inhibited nuclear protein synthesis. However, the application of photoreactivating light following dark holding with cycloheximide indicates that some steps of the repair of both nuclear and mitochondrial damage are performed in the absence of a synthesis of proteins.", "contents": "Protein synthesis and the recovery of both survival and cytoplasmic \"petite\" mutation in ultraviolet-treated yeast cells. I. Nuclear-directed protein synthesis. The contribution of nuclear-directed protein synthesis in the repair of lethal and mitochondrial genetic damage after UV-irradiation of exponential and stationary phage haploid yeast cells was examined. This was carried out using cycloheximide (CH), a specific inhibitor of nuclear protein synthesis. It appears that nuclear protein synthesis is required for the increase in survival seen after the liquid holding of cells at both stages, as well as for the \"petite\" recovery seen after the liquid holding of exponential phase cells. The characteristic negative liquid holding effect observed for the UV induction of \"petites\" in stationary phase cells (increase of the frequency of \"petites\" during storage) remained following all the treatments which inhibited nuclear protein synthesis. However, the application of photoreactivating light following dark holding with cycloheximide indicates that some steps of the repair of both nuclear and mitochondrial damage are performed in the absence of a synthesis of proteins."}
{"id": "PMID:1095920", "title": "Protein synthesis and the recovery of both survival and cytoplasmic \"petite\" mutation in ultraviolet-treated yeast cells. II. Mitochondrial protein synthesis.", "content": "The contribution of mitochondrial proteins in the repair of UV-induced lethal and cytoplasmic genetic damages was studied in dark liquid held exponential and stationary phase yeast cells. This was performed by using the specific inhibitors, erythromycin (ER) anc chloramphenicol (CAP). It was shown that mitochondrial proteins are involved in the recovery of stationary phase cells. Mitochondrial proteins are partly implicated in the mechanisms leading to the restoration of the (see article) genotype in UV-irradiated dark liquid held exponential phase cells. Here again, in stationary phase cells, mitochondrial enzymes do not seem to participate in the negative liquid holding (NLH) process for the (see article) induction, as shown by inhibiting mitochondrial protein synthesis or both mitochondrial and nuclear protein synthesis. When cells are grown in glycerol, the response after dark liquid holding of UV-treated cells in the different growth stages are similar to that found for glucose-grown cells. In other words, the fate of cytoplasmic genetic damage, in particular, is not correlated with the repressed or derepressed state of the mitochondria.", "contents": "Protein synthesis and the recovery of both survival and cytoplasmic \"petite\" mutation in ultraviolet-treated yeast cells. II. Mitochondrial protein synthesis. The contribution of mitochondrial proteins in the repair of UV-induced lethal and cytoplasmic genetic damages was studied in dark liquid held exponential and stationary phase yeast cells. This was performed by using the specific inhibitors, erythromycin (ER) anc chloramphenicol (CAP). It was shown that mitochondrial proteins are involved in the recovery of stationary phase cells. Mitochondrial proteins are partly implicated in the mechanisms leading to the restoration of the (see article) genotype in UV-irradiated dark liquid held exponential phase cells. Here again, in stationary phase cells, mitochondrial enzymes do not seem to participate in the negative liquid holding (NLH) process for the (see article) induction, as shown by inhibiting mitochondrial protein synthesis or both mitochondrial and nuclear protein synthesis. When cells are grown in glycerol, the response after dark liquid holding of UV-treated cells in the different growth stages are similar to that found for glucose-grown cells. In other words, the fate of cytoplasmic genetic damage, in particular, is not correlated with the repressed or derepressed state of the mitochondria."}
{"id": "PMID:1095921", "title": "Host-mediated assay with yeast and rats using probenecid (Benemid) to block the renal tubular excretion of cyclophosphamide metabolites.", "content": "The genetic activity of cyclophosphamide (Cy) was tested in the host-mediated assay (injection of yeasts into the peritoneal cavity of rats) modified by the use of probenecid (Pro) (Benemid) to block the renal tubular excretion of the genetically active metabolites. The genetic test system used was the induction of mitotic gene conversion in two unliked loci of a diploid strain of Saccharomyces cerevisiae. By this method the genetic effect of By was doubled in comparison with the case of administering Cy alone. Compared with the animals which received only Pro, increases of conversion frequencies of 20 times in the ade2 locus and of 15 times in the trp5 locus were found.", "contents": "Host-mediated assay with yeast and rats using probenecid (Benemid) to block the renal tubular excretion of cyclophosphamide metabolites. The genetic activity of cyclophosphamide (Cy) was tested in the host-mediated assay (injection of yeasts into the peritoneal cavity of rats) modified by the use of probenecid (Pro) (Benemid) to block the renal tubular excretion of the genetically active metabolites. The genetic test system used was the induction of mitotic gene conversion in two unliked loci of a diploid strain of Saccharomyces cerevisiae. By this method the genetic effect of By was doubled in comparison with the case of administering Cy alone. Compared with the animals which received only Pro, increases of conversion frequencies of 20 times in the ade2 locus and of 15 times in the trp5 locus were found."}
{"id": "PMID:1095922", "title": "Testing the mutagenic potency of chemical substances in a linear host-mediated assay. I. Experimental microbiological basis.", "content": "By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear-method is presented in which the population growth of the auxotrophic and substance-induced mutants used int he test can be described mathematically during their logarithmic growth phase with regression lines.", "contents": "Testing the mutagenic potency of chemical substances in a linear host-mediated assay. I. Experimental microbiological basis. By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear-method is presented in which the population growth of the auxotrophic and substance-induced mutants used int he test can be described mathematically during their logarithmic growth phase with regression lines."}
{"id": "PMID:1095923", "title": "Umbra limi: a model for the study of chromosome aberrations in fishes.", "content": "Due to the lack of information available on the effect of various clastogenic agents on the chromosomes of fishes, an in vivo cytogenetics model system was developed. The central mudminnow, Umbra limi, was chosen fro this study because of its ideal karyotype consisting of 22 large meta- and submetacentric chromosomes. Various organs of the fish were investigated to determine their suitability for chromosome preparations. The tissues of the intestines, stomach, kidneys, and gills were found to be the most suitable for clastogenic studies. Phase contrast observations were made on the chromosomes of control mudminnows and mudminnows exposed to 325 R of X-radiation. The control rate of spontaneous chromosome aberrations was found to be low (about 0.03%). In contrast, fish exposed to 325 R of X-rays had aberrations in approximately 30% of the metaphases per fish examined. An apparent increase in clumping and a decrease in the mitotic index were also noted. It was concluded that the chromosomes of Umbra limi displayed typical responses to low level radiation exposure and that this fish would be an ideal cytogenetics model for this study of induced chromosome aberrations in fishes.", "contents": "Umbra limi: a model for the study of chromosome aberrations in fishes. Due to the lack of information available on the effect of various clastogenic agents on the chromosomes of fishes, an in vivo cytogenetics model system was developed. The central mudminnow, Umbra limi, was chosen fro this study because of its ideal karyotype consisting of 22 large meta- and submetacentric chromosomes. Various organs of the fish were investigated to determine their suitability for chromosome preparations. The tissues of the intestines, stomach, kidneys, and gills were found to be the most suitable for clastogenic studies. Phase contrast observations were made on the chromosomes of control mudminnows and mudminnows exposed to 325 R of X-radiation. The control rate of spontaneous chromosome aberrations was found to be low (about 0.03%). In contrast, fish exposed to 325 R of X-rays had aberrations in approximately 30% of the metaphases per fish examined. An apparent increase in clumping and a decrease in the mitotic index were also noted. It was concluded that the chromosomes of Umbra limi displayed typical responses to low level radiation exposure and that this fish would be an ideal cytogenetics model for this study of induced chromosome aberrations in fishes."}
{"id": "PMID:1095924", "title": "The mutagenicity of saccharin.", "content": "Seventeen different reports are available dealing with the mutagenic effects of saccharin. Many of these are short abstracts, carrying incomplete information. Mainly tested as its sodium salt, saccharin has been found to be weakly mutagenic in Salmonella at very high doses, in Drosophila at moderate doses, and in mice at moderate to high doses. The compound is a weak chromosome breaker in onion root tips and in Chinese hamster cells. For most of these, and for other test systems as well, a number of doubtful or negative results have also been reported. Altogether the evidence for chromosome-breaking properties is stronger than for the induction of point mutations. The overall picture is too conflicting and equivocal to classify saccharin as a proven mutagen. It is suggested that the observed contradictions might be related to the occurrence of varying amounts of impurities.", "contents": "The mutagenicity of saccharin. Seventeen different reports are available dealing with the mutagenic effects of saccharin. Many of these are short abstracts, carrying incomplete information. Mainly tested as its sodium salt, saccharin has been found to be weakly mutagenic in Salmonella at very high doses, in Drosophila at moderate doses, and in mice at moderate to high doses. The compound is a weak chromosome breaker in onion root tips and in Chinese hamster cells. For most of these, and for other test systems as well, a number of doubtful or negative results have also been reported. Altogether the evidence for chromosome-breaking properties is stronger than for the induction of point mutations. The overall picture is too conflicting and equivocal to classify saccharin as a proven mutagen. It is suggested that the observed contradictions might be related to the occurrence of varying amounts of impurities."}
{"id": "PMID:1095928", "title": "Factors affecting experimental infection with Cryptococcus neoformans in mice with special reference to an endotoxic substance of C. neoformans.", "content": "A close correlation was observed between body weight and length of the survival time of mice inoculated intravenously (i.v.) with Cryptococcus neoformans (p less than 0.001). An endotoxic substance of C. neoformans (Cr-ET) increased the susceptibility of mice to i.v. infection of C. neoformans only when more than 50 mug of Cr-ET was injected i.v. 24 hours before infection. Intraperitoneal (i.p.) administration of dimethyl sulfoxide which is found to enhance bacterial infection did not enhance death rate of mice infected i.p. with C. neoformans.", "contents": "Factors affecting experimental infection with Cryptococcus neoformans in mice with special reference to an endotoxic substance of C. neoformans. A close correlation was observed between body weight and length of the survival time of mice inoculated intravenously (i.v.) with Cryptococcus neoformans (p less than 0.001). An endotoxic substance of C. neoformans (Cr-ET) increased the susceptibility of mice to i.v. infection of C. neoformans only when more than 50 mug of Cr-ET was injected i.v. 24 hours before infection. Intraperitoneal (i.p.) administration of dimethyl sulfoxide which is found to enhance bacterial infection did not enhance death rate of mice infected i.p. with C. neoformans."}
{"id": "PMID:1095929", "title": "[Antibiosis shown by a strain of Byssochlamys nivea Westling, 1909. III. Chromatographic characteristics and isolation of the active fraction].", "content": "Early papers revealed that culture filtrate of Byssochlamys nivea has antibiotic properties. Active filtrate of B. nivea is extracted with ethyl acetate. Paper chromatography is carried out on Whatman no. 1; thin-layer chromatography is carried out on Kieselgel; visualization with bioautographic technique reveals a single spot. Ethyl acetate extract is passed through silicic acid-celite column. Each isolated fraction is tested for antibiotic activity. Only one supports this activity; a crystallized matter, melting point 109-110 degrees C, thermostable, is obtained.", "contents": "[Antibiosis shown by a strain of Byssochlamys nivea Westling, 1909. III. Chromatographic characteristics and isolation of the active fraction]. Early papers revealed that culture filtrate of Byssochlamys nivea has antibiotic properties. Active filtrate of B. nivea is extracted with ethyl acetate. Paper chromatography is carried out on Whatman no. 1; thin-layer chromatography is carried out on Kieselgel; visualization with bioautographic technique reveals a single spot. Ethyl acetate extract is passed through silicic acid-celite column. Each isolated fraction is tested for antibiotic activity. Only one supports this activity; a crystallized matter, melting point 109-110 degrees C, thermostable, is obtained."}
{"id": "PMID:1095932", "title": "Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli.", "content": "Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA.", "contents": "Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA."}
{"id": "PMID:1095935", "title": "Relation between the amount of smooth muscle of venous tissue and the degree of supersensitivity to isoprenaline caused by inhibition of catechol-O-methyl transferase.", "content": "The relation between the smooth muscle cell mass of dog saphenous vein strips and the degree of supersensitivity to isoprenaline caused by U-0521 (3,4-dihydroxy-2-methyl propiophenone), an inhibitor of the catechol-O-methyl transferase (COMT), was studied. For the quantitative determination of smooth muscle mass, the thickness of the muscle layer as determined by light microscopy and the maximal shortening induced by supramaximal concentration of phenylephrine were used. After the strips had been contracted by 3x10-6M phenylephrine, a concentration which was able to produce an about 90% maximal contraction, dose-response curves to the relaxant effect of isoprenaline were determined in the absence and in the presence of U-0521 (10-4M). It was observed that U-0521 caused marked supersensitivity to the relaxant effect of isoprenaline (varying between 3 and 81 times), as well as an increase of the  maximal relaxation caused by this amine (varying between 7 and 120%). The correlation between these data and the smooth muscle cell mass shows that there was a direct proportionality between these parameters. Oxytetracycline (10-4M), an inhibitor of binding of catecholamines to collagen, did not produce any enhancement of the effects of isoprenaline. It is concluded that COMT is related to smooth muscle cells in this tissue.", "contents": "Relation between the amount of smooth muscle of venous tissue and the degree of supersensitivity to isoprenaline caused by inhibition of catechol-O-methyl transferase. The relation between the smooth muscle cell mass of dog saphenous vein strips and the degree of supersensitivity to isoprenaline caused by U-0521 (3,4-dihydroxy-2-methyl propiophenone), an inhibitor of the catechol-O-methyl transferase (COMT), was studied. For the quantitative determination of smooth muscle mass, the thickness of the muscle layer as determined by light microscopy and the maximal shortening induced by supramaximal concentration of phenylephrine were used. After the strips had been contracted by 3x10-6M phenylephrine, a concentration which was able to produce an about 90% maximal contraction, dose-response curves to the relaxant effect of isoprenaline were determined in the absence and in the presence of U-0521 (10-4M). It was observed that U-0521 caused marked supersensitivity to the relaxant effect of isoprenaline (varying between 3 and 81 times), as well as an increase of the  maximal relaxation caused by this amine (varying between 7 and 120%). The correlation between these data and the smooth muscle cell mass shows that there was a direct proportionality between these parameters. Oxytetracycline (10-4M), an inhibitor of binding of catecholamines to collagen, did not produce any enhancement of the effects of isoprenaline. It is concluded that COMT is related to smooth muscle cells in this tissue."}
{"id": "PMID:1095951", "title": "Measurement of plasma and hypothalamic luteinizing hormon-releasing hormone in pregnant mare serum-induced ovulating immature rats.", "content": "A radioimmunoassay for luteinizing hormone-releasing hormone (LH-RH) is described which is capable of detecting LH-RH in plasma and brain tissues. Fluctuations in plasma and hypothalamic LH-RH as well as plasma LH were measured in immature female rats in which ovulation was induced by a single injection of pregnant mare serum (PMS). High plasma levels of LH-RH were found 47-49 h after the initial injection of PMS, coincident with high plasma LH concentrations. However plasma LH-RH levels were considerably lower at 55-57 h post-PMS, when plasma LH levels were elevated. Hypothalamic LH-RH content could not be correlated with either plasma LH-RH or LH levels.", "contents": "Measurement of plasma and hypothalamic luteinizing hormon-releasing hormone in pregnant mare serum-induced ovulating immature rats. A radioimmunoassay for luteinizing hormone-releasing hormone (LH-RH) is described which is capable of detecting LH-RH in plasma and brain tissues. Fluctuations in plasma and hypothalamic LH-RH as well as plasma LH were measured in immature female rats in which ovulation was induced by a single injection of pregnant mare serum (PMS). High plasma levels of LH-RH were found 47-49 h after the initial injection of PMS, coincident with high plasma LH concentrations. However plasma LH-RH levels were considerably lower at 55-57 h post-PMS, when plasma LH levels were elevated. Hypothalamic LH-RH content could not be correlated with either plasma LH-RH or LH levels."}
{"id": "PMID:1095952", "title": "Prostaglandin E2-induced release of luteinizing hormone-releasing factor (LRF).", "content": "Prostaglandin E2 (PGE2) injected into the third ventricle (3rd V) of conscious ovariectomized rats bearing a permanent jugular cannula increased the percentage of plasma samples showing detectable immunoassayable LRF levels at 1, 3 and 5 min after injection. This percentage was small at 2 and 4 min. When plasma LRF and LH titers were measured in animals injected intraventicularly with PGE2 and decapitated 5 min later, both LRF and LH were significantly higher than control values of diluent-injected animals. These results indicate that PGE2 is acting on the hypothalamus to induce release of LRF sufficient in mangitude to be detected by measuring this neurohormone directly in peripheral plasma.", "contents": "Prostaglandin E2-induced release of luteinizing hormone-releasing factor (LRF). Prostaglandin E2 (PGE2) injected into the third ventricle (3rd V) of conscious ovariectomized rats bearing a permanent jugular cannula increased the percentage of plasma samples showing detectable immunoassayable LRF levels at 1, 3 and 5 min after injection. This percentage was small at 2 and 4 min. When plasma LRF and LH titers were measured in animals injected intraventicularly with PGE2 and decapitated 5 min later, both LRF and LH were significantly higher than control values of diluent-injected animals. These results indicate that PGE2 is acting on the hypothalamus to induce release of LRF sufficient in mangitude to be detected by measuring this neurohormone directly in peripheral plasma."}
{"id": "PMID:1095953", "title": "Modification of brain and CSF LH-RH following deafferentation.", "content": "The effect of hypothalamic deafferentation upon levels of LH-RH in brain, cerebrospinal fluid, and plasma was studied in adult male rats. Deafferentation produced a significant increase in the LH-RH concentration of third ventricular CSF (from 140.6 to 343.9 pg/microl), while there was no change in median eminence or hypothalamic content. LH-RH in the pineal gland and the posterior pituitary was also elevated by hypothalamic isolation.", "contents": "Modification of brain and CSF LH-RH following deafferentation. The effect of hypothalamic deafferentation upon levels of LH-RH in brain, cerebrospinal fluid, and plasma was studied in adult male rats. Deafferentation produced a significant increase in the LH-RH concentration of third ventricular CSF (from 140.6 to 343.9 pg/microl), while there was no change in median eminence or hypothalamic content. LH-RH in the pineal gland and the posterior pituitary was also elevated by hypothalamic isolation."}
{"id": "PMID:1095954", "title": "HMOs, competition, and government.", "content": "This article considers the role of three sets of forces affecting the development of health maintenance organizations (HMOs) during the early 1970s: legal restrictions, market conditions, and the federal government's policy stance. Our review of the evidence suggests that the rapid increase in the number of HMOs during this period was primarily due to favorable market conditions in certain areas of the country combined with a highly encouraging federal policy toward HMOs. Legal restrictions do not appear to have been as serious a barrier to HMO development as was earlier believed. In 1973-74, major new legislation was enacted at both the federal and state levels, ostensibly to encourage HMO development. Our review of this legislation suggests that, while it removes many of the old legal requirements which apparently were not serious barriers to HMO development, the new legislation imposes a host of new conditions and requirements on HMO participation in the health care marketplace. Ironically, some of these new features may impede the operation of the very market forces which encouraged the earlier HMO growth.", "contents": "HMOs, competition, and government. This article considers the role of three sets of forces affecting the development of health maintenance organizations (HMOs) during the early 1970s: legal restrictions, market conditions, and the federal government's policy stance. Our review of the evidence suggests that the rapid increase in the number of HMOs during this period was primarily due to favorable market conditions in certain areas of the country combined with a highly encouraging federal policy toward HMOs. Legal restrictions do not appear to have been as serious a barrier to HMO development as was earlier believed. In 1973-74, major new legislation was enacted at both the federal and state levels, ostensibly to encourage HMO development. Our review of this legislation suggests that, while it removes many of the old legal requirements which apparently were not serious barriers to HMO development, the new legislation imposes a host of new conditions and requirements on HMO participation in the health care marketplace. Ironically, some of these new features may impede the operation of the very market forces which encouraged the earlier HMO growth."}
{"id": "PMID:1095955", "title": "The effects of prepayment on access to medical care: the PACC experience.", "content": "The data reported herein are taken from a larger study in which a prepaid medical foundation was compared with a non-prepaid free-for-service system on a number of factors pertaining to how health care is perceived by both Medicaid recipients and physicians. The data to be presented are confined to the issue of the impact of prepayment on Medicaid recipients' perceptions of their access to health care. Two sets of questions are explored. The first set bears directly on the issue of gaining access to care. The second set addresses the issue of the acceptability of the services received. Few differences were observed between the systems in either accessibility or acceptability. Thus, the fears of some critics of the HMO concept with respect to prepayment creating incentives for the denial of services are not supported by the data. It is concluded that the organizational features of medical practice which affect access are actually quite similar in the two systems.", "contents": "The effects of prepayment on access to medical care: the PACC experience. The data reported herein are taken from a larger study in which a prepaid medical foundation was compared with a non-prepaid free-for-service system on a number of factors pertaining to how health care is perceived by both Medicaid recipients and physicians. The data to be presented are confined to the issue of the impact of prepayment on Medicaid recipients' perceptions of their access to health care. Two sets of questions are explored. The first set bears directly on the issue of gaining access to care. The second set addresses the issue of the acceptability of the services received. Few differences were observed between the systems in either accessibility or acceptability. Thus, the fears of some critics of the HMO concept with respect to prepayment creating incentives for the denial of services are not supported by the data. It is concluded that the organizational features of medical practice which affect access are actually quite similar in the two systems."}
{"id": "PMID:1095960", "title": "[Some elective procedures for cranioplasty].", "content": "The literature methods for cranioplasty in the repair of losses of bone substance are described. Autologous bone grafts from the iliac ala or the ribs are used at the Turin University Reconstructive Plastic Surgery Department. A method using solid silicone is also employed and will be discussed in greater detail in a subsequent paper. Three illustrative cases are presented.", "contents": "[Some elective procedures for cranioplasty]. The literature methods for cranioplasty in the repair of losses of bone substance are described. Autologous bone grafts from the iliac ala or the ribs are used at the Turin University Reconstructive Plastic Surgery Department. A method using solid silicone is also employed and will be discussed in greater detail in a subsequent paper. Three illustrative cases are presented."}
{"id": "PMID:1095961", "title": "[Aspects of humoral immunity in ulcerous colitis].", "content": "Various immunological indices, hitherto considered in isolation, were evaluated in a single group of 20 cases of ulcerous colitis. Immunofluorescence showed anticolon Ab in 3 cases (15%), whereas this features was absent in a control group. Antistomach, antithyroid and non-organ-specific Ab's (ANA, AMA, SMA) were equally incident in both groups. One caso is examined separately on account of its simultaneous presentation of human and rat antistomach and antithyroid and antismooth muscle Ab's. Reuma test, Waaler Roose reaction, L.E. phenomenon and test, and direct and indirect Coombs test results were negative in nearly all cases. Slight quantitative changes in serum proteins revealed by electrophoresis and immunodiffusion were indicative of inflammation with an immunitary tendency. The data are not sufficient to support the attribution of an autoimmunitary aetiology to ulcerous colitis, though simultaneous evaluation of reaction indices serves to strengthen this view.", "contents": "[Aspects of humoral immunity in ulcerous colitis]. Various immunological indices, hitherto considered in isolation, were evaluated in a single group of 20 cases of ulcerous colitis. Immunofluorescence showed anticolon Ab in 3 cases (15%), whereas this features was absent in a control group. Antistomach, antithyroid and non-organ-specific Ab's (ANA, AMA, SMA) were equally incident in both groups. One caso is examined separately on account of its simultaneous presentation of human and rat antistomach and antithyroid and antismooth muscle Ab's. Reuma test, Waaler Roose reaction, L.E. phenomenon and test, and direct and indirect Coombs test results were negative in nearly all cases. Slight quantitative changes in serum proteins revealed by electrophoresis and immunodiffusion were indicative of inflammation with an immunitary tendency. The data are not sufficient to support the attribution of an autoimmunitary aetiology to ulcerous colitis, though simultaneous evaluation of reaction indices serves to strengthen this view."}
{"id": "PMID:1095962", "title": "[S-Adenosylmethionine: plasma levels in hepatic cirrhosis and preliminary results of its clinical use in hepatology. Double-blind study].", "content": "Since S-adenosylmethionine (SAMe) plasma levels are highly reduced in cirrhotic patients, this, showing that a more or less overt deficiency of SAMe-dependent biological transmethylations does exist in the hepatocyte pathology, mostley affecting the albuminopoyesis. Treatment with 15 mg SAMe i.v. or i.m. administered four times a day for 30 days' period, induced in 15 patients with hepatic cirrhosis a statistically significant improvement of the afore mentioned livel cell function, albuminopoyesis: a significant improvement was also observed in the other biohumoral parameters considered to test hepatic function. Administration of equimolecular (with respect to SAMe) doses of L-methionine and ATP to a group of 15 cirrhotic patients under clinical conditions similar to those of the group previously studied, induced none of the modifications observed in the latter. This proved that the therapeutic effects are due only to S-adenosylmethionine.", "contents": "[S-Adenosylmethionine: plasma levels in hepatic cirrhosis and preliminary results of its clinical use in hepatology. Double-blind study]. Since S-adenosylmethionine (SAMe) plasma levels are highly reduced in cirrhotic patients, this, showing that a more or less overt deficiency of SAMe-dependent biological transmethylations does exist in the hepatocyte pathology, mostley affecting the albuminopoyesis. Treatment with 15 mg SAMe i.v. or i.m. administered four times a day for 30 days' period, induced in 15 patients with hepatic cirrhosis a statistically significant improvement of the afore mentioned livel cell function, albuminopoyesis: a significant improvement was also observed in the other biohumoral parameters considered to test hepatic function. Administration of equimolecular (with respect to SAMe) doses of L-methionine and ATP to a group of 15 cirrhotic patients under clinical conditions similar to those of the group previously studied, induced none of the modifications observed in the latter. This proved that the therapeutic effects are due only to S-adenosylmethionine."}
{"id": "PMID:1095963", "title": "[Relations between protidopoiesis and biological transmethylations: action of S-adenosylmethionine on protein crasis in chronic hepatopathies].", "content": "After a diffuse introductory discussion on S-adenosylmethionine methyltransferase activity, the results of an experimental trial carried out on 70 hospitalized patients with chronic hepatitis either persistent or aggressive, and with hepatic cirrhosis at various degrees, are reported. A first group of patients was treated with SAMe (S-adenosylmethionine) intravenously administered for 20 days at two daily doses of 15 mg. The second group was instead-receiving, still by i.v. route, 20 mg of fructose-1-6-diphosphage sodium salt, as a drug for comparison given twice a day at the doses of 2.5 over a 20 days' period. The protidemia picture and in particular the albuminic fraction, generally altered in all the cases under study, have been rapidly and significantly restored only in the group of patients treated with SAMe this indicating the efficacy of this molecule on the liver function.", "contents": "[Relations between protidopoiesis and biological transmethylations: action of S-adenosylmethionine on protein crasis in chronic hepatopathies]. After a diffuse introductory discussion on S-adenosylmethionine methyltransferase activity, the results of an experimental trial carried out on 70 hospitalized patients with chronic hepatitis either persistent or aggressive, and with hepatic cirrhosis at various degrees, are reported. A first group of patients was treated with SAMe (S-adenosylmethionine) intravenously administered for 20 days at two daily doses of 15 mg. The second group was instead-receiving, still by i.v. route, 20 mg of fructose-1-6-diphosphage sodium salt, as a drug for comparison given twice a day at the doses of 2.5 over a 20 days' period. The protidemia picture and in particular the albuminic fraction, generally altered in all the cases under study, have been rapidly and significantly restored only in the group of patients treated with SAMe this indicating the efficacy of this molecule on the liver function."}
{"id": "PMID:1095964", "title": "[Double-blind polycentric study of the action of S-adenosylmethionine in hepatic cirrhosis].", "content": "Two comparable groups of patients with hepatic cirrhosis of different genesis in a compensation phase have been treated for 30 days with S-adenosylmethionine and vitamine B-12 (28 cases) or with vitamine B-12 alone (25 cases). The drugs were given by slow intravenous route at the daily dose of 150 mg of SAMe and 2000 gamma of vit. B-12 or of 2000 gamma of vit. B-12 alone, in two adminstrations. An evaluation of the results was carried out mostly on the laboratory data testing the liver function. Only the group of patients who had received SAMe showed significant modifications of all the parameters considered. This is confirming SAMe ability to restore hepatocyte activity bringing also to normal the protein synthesis.", "contents": "[Double-blind polycentric study of the action of S-adenosylmethionine in hepatic cirrhosis]. Two comparable groups of patients with hepatic cirrhosis of different genesis in a compensation phase have been treated for 30 days with S-adenosylmethionine and vitamine B-12 (28 cases) or with vitamine B-12 alone (25 cases). The drugs were given by slow intravenous route at the daily dose of 150 mg of SAMe and 2000 gamma of vit. B-12 or of 2000 gamma of vit. B-12 alone, in two adminstrations. An evaluation of the results was carried out mostly on the laboratory data testing the liver function. Only the group of patients who had received SAMe showed significant modifications of all the parameters considered. This is confirming SAMe ability to restore hepatocyte activity bringing also to normal the protein synthesis."}
{"id": "PMID:1095965", "title": "[Double-blind studies of the therapeutic action of S-Adenosylmethionine (SAMe) in oral administration, in liver cirrhosis and other chronic hepatitides].", "content": "Six oral administrations per day of 30 mg S-adenosylmethionine (SAMe) for 30 days, in addition to 6000 gamma/day of Vitamine B12 induced marked improvements of biochemical parameters in 20 patients with hepatic cirrhosis or various chronic hepatites. Particularly, the protidemia, bilirubinemia and radial immunodiffusion have shown the highest favorable drug responses. These improvements were still lasting and even further increasing 30 days after the end of therapy. In another group of patients with similar diagnosis and under clinical conditions comparable to the previous group of twenty, the administration of Vitamine B12 alone, in the same doses as above, has not induced any alteration in the biochemical parameters.", "contents": "[Double-blind studies of the therapeutic action of S-Adenosylmethionine (SAMe) in oral administration, in liver cirrhosis and other chronic hepatitides]. Six oral administrations per day of 30 mg S-adenosylmethionine (SAMe) for 30 days, in addition to 6000 gamma/day of Vitamine B12 induced marked improvements of biochemical parameters in 20 patients with hepatic cirrhosis or various chronic hepatites. Particularly, the protidemia, bilirubinemia and radial immunodiffusion have shown the highest favorable drug responses. These improvements were still lasting and even further increasing 30 days after the end of therapy. In another group of patients with similar diagnosis and under clinical conditions comparable to the previous group of twenty, the administration of Vitamine B12 alone, in the same doses as above, has not induced any alteration in the biochemical parameters."}
{"id": "PMID:1095981", "title": "Diarrhoea: a traveller's tale.", "content": "Travellers' diarrhoea is a result of travelling overseas. Proper diagnosis can only be made when a full microbiological investigation is carried. A case due to Shigella flexneri is described.", "contents": "Diarrhoea: a traveller's tale. Travellers' diarrhoea is a result of travelling overseas. Proper diagnosis can only be made when a full microbiological investigation is carried. A case due to Shigella flexneri is described."}
{"id": "PMID:1095987", "title": "Perinatal aspects of bilirubin metabolism.", "content": "Study of bilirubin metabolism in the fetus and newborn continues to reveal new insights into fetal physiology and maternal-fetal-neonatal relationships. Therapeutic advances directed toward prevention as well as treatment of hyperbilirubinemia continue to expand our understanding of both the basic metabolic pathways and previously unsuspected interplay between host and environment. Seemingly insignificant alterations in temperature, oxygen, carbohydrate, or light itself have been shown to affect the delicate balances in bilirubin metabolism. Similar impact upon other developing systems undoubtedly occur. Those who accept responsibility for the care of the fetus and newborn must recognize that all we do and all we fail to do in their care has a much greater potential for affecting the quality of life than at any other time.", "contents": "Perinatal aspects of bilirubin metabolism. Study of bilirubin metabolism in the fetus and newborn continues to reveal new insights into fetal physiology and maternal-fetal-neonatal relationships. Therapeutic advances directed toward prevention as well as treatment of hyperbilirubinemia continue to expand our understanding of both the basic metabolic pathways and previously unsuspected interplay between host and environment. Seemingly insignificant alterations in temperature, oxygen, carbohydrate, or light itself have been shown to affect the delicate balances in bilirubin metabolism. Similar impact upon other developing systems undoubtedly occur. Those who accept responsibility for the care of the fetus and newborn must recognize that all we do and all we fail to do in their care has a much greater potential for affecting the quality of life than at any other time."}
{"id": "PMID:1095989", "title": "Principles of adolescent gynecology.", "content": "Most of the gynecologic problems presented by adolescent girls are dysfunctional. They include, in order of frequency: dysfunctional uterine bleeding, dysmenorrhea, and amenorrhea. Leukorrhea is also very likely to be dysfunctional, in that in many cases it is not a true vaginal discharge but an increase in vaginal secretion. For the most part, gynecologic disorders in adolescent girls are diagnosed and treated in the same fashion as similar conditions in adult women. Genital tumors are managed in accordance with accepted gynecologic principles. Inasmuch as the gynecologic disorders encountered in adolescent patients are similar to comparable ones in adult women, these problems properly belong in the realm of the gynecologist, who is best equipped to diagnose and treat them.", "contents": "Principles of adolescent gynecology. Most of the gynecologic problems presented by adolescent girls are dysfunctional. They include, in order of frequency: dysfunctional uterine bleeding, dysmenorrhea, and amenorrhea. Leukorrhea is also very likely to be dysfunctional, in that in many cases it is not a true vaginal discharge but an increase in vaginal secretion. For the most part, gynecologic disorders in adolescent girls are diagnosed and treated in the same fashion as similar conditions in adult women. Genital tumors are managed in accordance with accepted gynecologic principles. Inasmuch as the gynecologic disorders encountered in adolescent patients are similar to comparable ones in adult women, these problems properly belong in the realm of the gynecologist, who is best equipped to diagnose and treat them."}
{"id": "PMID:1095992", "title": "Urologic injuries.", "content": "Urologic injury during pelvic surgery is primarily a matter of prevention; should it occur, however, prompt recognition and appropriate management are essential. Basic principles are applicable, whether the operation has been performed via the abdominal or the vaginal route. Conditions such as endometriosis, infection, previous operation, and tumor distortion increase the likelihood of damage to the urinary tract. Difficult dissection may easily damage the urethra, bladder, or ureters. Preliminary information as to the position of the ureters and bladder will alert the surgeon. Exacting identification of the ureter and bladder is necessary prior to the use of clamps, sutures, or incision. The next most important factor is recognition of such injury. Whenever the possibility exists, it must be promptly and definitively delineated. Injury to the urethra, bladder, or ureters, when recognized and appropriately repaired, is unlikely to cause subsequent complications. Good surgical techniques, adequate and accurate hemostasis, prevention or treatment of infection, drainage of the operative site, and splinting and resting of the organ are necessary. It is the unrecognized and unrepaired urologic injury that increases the chances of immediate and delayed complications. Peritonitis, infection, stricture, fistula, hydronephrosis, and renal damage as well as death may occur even if the patient survives the immediate postoperative complications. It is important in such instances that the error not be compounded by rash attempts at additional surgical procedures at an operative site where there is maximal tissue reaction. General supportive measures with drainage of the urinary tract to preserve renal function are all that should be done. It is likewise important that the surgeon not be rushed into an early attempt to correct the resultant fistula. Adequate time must be allowed for the tissues to heal so that the subsequent repair may be carried out successfully and without further complications.", "contents": "Urologic injuries. Urologic injury during pelvic surgery is primarily a matter of prevention; should it occur, however, prompt recognition and appropriate management are essential. Basic principles are applicable, whether the operation has been performed via the abdominal or the vaginal route. Conditions such as endometriosis, infection, previous operation, and tumor distortion increase the likelihood of damage to the urinary tract. Difficult dissection may easily damage the urethra, bladder, or ureters. Preliminary information as to the position of the ureters and bladder will alert the surgeon. Exacting identification of the ureter and bladder is necessary prior to the use of clamps, sutures, or incision. The next most important factor is recognition of such injury. Whenever the possibility exists, it must be promptly and definitively delineated. Injury to the urethra, bladder, or ureters, when recognized and appropriately repaired, is unlikely to cause subsequent complications. Good surgical techniques, adequate and accurate hemostasis, prevention or treatment of infection, drainage of the operative site, and splinting and resting of the organ are necessary. It is the unrecognized and unrepaired urologic injury that increases the chances of immediate and delayed complications. Peritonitis, infection, stricture, fistula, hydronephrosis, and renal damage as well as death may occur even if the patient survives the immediate postoperative complications. It is important in such instances that the error not be compounded by rash attempts at additional surgical procedures at an operative site where there is maximal tissue reaction. General supportive measures with drainage of the urinary tract to preserve renal function are all that should be done. It is likewise important that the surgeon not be rushed into an early attempt to correct the resultant fistula. Adequate time must be allowed for the tissues to heal so that the subsequent repair may be carried out successfully and without further complications."}
{"id": "PMID:1095995", "title": "Effect of LHRH stimulation in anovulatory women.", "content": "Serum FSH and LH and levels were determined at different time intervals after the subcutaneous administration of 100 mug LHRH to 24 females. Serum FSH levels were of the same order in the oligomenorrhea-amenorrhea  and the Stein-Leventhal group of patients but elevated in gonadal failure. There was a great degree of overlap between serum LH values in the three groups. Gondal failure patients, however, could be distinguished from the other  two groups because these patients had an elevation of both serum FSH and LH, and the response to LHRH was considereably exaggerated in comparison to the other two groups.", "contents": "Effect of LHRH stimulation in anovulatory women. Serum FSH and LH and levels were determined at different time intervals after the subcutaneous administration of 100 mug LHRH to 24 females. Serum FSH levels were of the same order in the oligomenorrhea-amenorrhea  and the Stein-Leventhal group of patients but elevated in gonadal failure. There was a great degree of overlap between serum LH values in the three groups. Gondal failure patients, however, could be distinguished from the other  two groups because these patients had an elevation of both serum FSH and LH, and the response to LHRH was considereably exaggerated in comparison to the other two groups."}
{"id": "PMID:1095996", "title": "Postmenopausal symptomatology, maturation index, and plasma estrogen levels.", "content": "An attempt to correlate postmenopausal symptoms with the vaginal maturation index and plasma levels of estrogen was made in a group of patients with surgical menopause. The group was followed up monthly for 6 months in a double-blind study during which the patients received equine esterified estrogen and synthetic esterfield estrogens. No statistical difference in plasma levels of estrogen was  found between the group of patients with symptoms and the patients without symptoms, with or without estrogen therapy. There was no correlation of the maturation index with the presence or absence of symptoms or  with the estrogen plasma levels.", "contents": "Postmenopausal symptomatology, maturation index, and plasma estrogen levels. An attempt to correlate postmenopausal symptoms with the vaginal maturation index and plasma levels of estrogen was made in a group of patients with surgical menopause. The group was followed up monthly for 6 months in a double-blind study during which the patients received equine esterified estrogen and synthetic esterfield estrogens. No statistical difference in plasma levels of estrogen was  found between the group of patients with symptoms and the patients without symptoms, with or without estrogen therapy. There was no correlation of the maturation index with the presence or absence of symptoms or  with the estrogen plasma levels."}
{"id": "PMID:1096003", "title": "Yeasts in banal external ocular inflammations.", "content": "In 53 out of 313 patients suffering from longstanding inflammations of the outer eye or dacryocanaliculitis, not yielding to the obligate treatment with antibiotics and steroids, yeasts were isolated and regarded as the causative agents. Among the yeasts, different Candida species were prevalent, with C. albicans and C. guillermondii at the top. An obstinate, superificial epithelial and subepithelial punctate keratitis appeared to represent an entity pathognomonic of a yeast infection. Eradication of the yeasts and cure of the inflammation was achieved only by intensive prolonged treatment with Mycostatin or amphotericin B.", "contents": "Yeasts in banal external ocular inflammations. In 53 out of 313 patients suffering from longstanding inflammations of the outer eye or dacryocanaliculitis, not yielding to the obligate treatment with antibiotics and steroids, yeasts were isolated and regarded as the causative agents. Among the yeasts, different Candida species were prevalent, with C. albicans and C. guillermondii at the top. An obstinate, superificial epithelial and subepithelial punctate keratitis appeared to represent an entity pathognomonic of a yeast infection. Eradication of the yeasts and cure of the inflammation was achieved only by intensive prolonged treatment with Mycostatin or amphotericin B."}
{"id": "PMID:1096004", "title": "Lymphoproliferative disease of the hard palate: a clinicopathologic entity. A study of twenty-one cases.", "content": "Twenty-one cases of a lymphoproliferative disorder believed to represent a clinico-pathologic entity were studied. These lesions, which appeared on the hard palate, occurred in elderly men and women with an average age of 70 years. The lesions presented as soft, fluctuant swellings which were occasionally bilateral, ulcerated, or discolored. Microscopically, the lesions were classified as lymphocytic lymphomas; eight were diffuse and well differentiated, seven were nodular and well differentiated, and six were diffuse and poorly differentiated. All lesions were characterized by a tendency of the lymphocytes to show nuclear fragility. Accessory salivary gland involvement was common, this being characterized by acinar obliteration to varying degrees and by periductal hyalinization of collagen. Determinate follow-up of fourteen patients disclosed that eight died of disseminated lymphoma, three are alive with disease, and three are free of disease after treatment with radiation and/or chemotherapy. Lymphoproliferative lesions in this location are believed to be malignant or potentially malignant processes.", "contents": "Lymphoproliferative disease of the hard palate: a clinicopathologic entity. A study of twenty-one cases. Twenty-one cases of a lymphoproliferative disorder believed to represent a clinico-pathologic entity were studied. These lesions, which appeared on the hard palate, occurred in elderly men and women with an average age of 70 years. The lesions presented as soft, fluctuant swellings which were occasionally bilateral, ulcerated, or discolored. Microscopically, the lesions were classified as lymphocytic lymphomas; eight were diffuse and well differentiated, seven were nodular and well differentiated, and six were diffuse and poorly differentiated. All lesions were characterized by a tendency of the lymphocytes to show nuclear fragility. Accessory salivary gland involvement was common, this being characterized by acinar obliteration to varying degrees and by periductal hyalinization of collagen. Determinate follow-up of fourteen patients disclosed that eight died of disseminated lymphoma, three are alive with disease, and three are free of disease after treatment with radiation and/or chemotherapy. Lymphoproliferative lesions in this location are believed to be malignant or potentially malignant processes."}
{"id": "PMID:1096031", "title": "Induced hypervitaminemia A test and its standards in adults.", "content": "Oral administration of large doses of vitamin A permits one to study pos-prandial lipemia. Thanks to a new fluorescimetric and automatic method, estimation of vitamin A is now much easier than formerly. The normal results of the oral vitamin A tolerance test are evaluated with this new method and compared with those given by the previous method.", "contents": "Induced hypervitaminemia A test and its standards in adults. Oral administration of large doses of vitamin A permits one to study pos-prandial lipemia. Thanks to a new fluorescimetric and automatic method, estimation of vitamin A is now much easier than formerly. The normal results of the oral vitamin A tolerance test are evaluated with this new method and compared with those given by the previous method."}
{"id": "PMID:1096032", "title": "[Origin, nature, role and fate of prostaglandins liberated during the expansion of intravascular space in the anesthetized rat].", "content": "In experiments in which blood was cross-circulating in rats, the blood pressure of the recipient dropped while that of the donor rose, following the increase of the circulating blood volume, produced by infusion either of saline or blood. The phenomenon was almost imperceptible when binephrectomized animals were used. In experiments in which the blood-bathed organ technique was used, prostaglandin-like substances were detected, released during the rise of the blood pressure, produced by the same stimulus (the expansion), in anaesthetized rats. A significant difference was found between the prostaglandin-like substances detected using the blood-bathed organ technique, in normal rats (5.387 ng per ml of blood plus or minus 0.288 = SEM) and those detected in binephrectomized rats (3.202 ng per ml of blood plus or minus 0.330, p smaller than 0.025). The biologically active substances detected in 25 ml of blood collected during expansion, while the assay organs showed a prostaglandin-like activity, were found to have the chromatographic behaviour and the bioassay properties of PGA, PGE and PGF series. A great quantity of the biologically active substances, having the chromatographic behaviour and the bioassay properties of PGA, PGS and PGF was detected in the rat renal medulla. Sufficient quantities of the released prostaglandin-like substances could escape the pulmonary vascular bed in this species of animal. It was concluded that a great quantity of the released prostaglandin-like substances came from the kidney and their release by this particular mechanism suggested that they play an important homeostatic role on the blood pressure, blood volume, and sodium and water balance regulation.", "contents": "[Origin, nature, role and fate of prostaglandins liberated during the expansion of intravascular space in the anesthetized rat]. In experiments in which blood was cross-circulating in rats, the blood pressure of the recipient dropped while that of the donor rose, following the increase of the circulating blood volume, produced by infusion either of saline or blood. The phenomenon was almost imperceptible when binephrectomized animals were used. In experiments in which the blood-bathed organ technique was used, prostaglandin-like substances were detected, released during the rise of the blood pressure, produced by the same stimulus (the expansion), in anaesthetized rats. A significant difference was found between the prostaglandin-like substances detected using the blood-bathed organ technique, in normal rats (5.387 ng per ml of blood plus or minus 0.288 = SEM) and those detected in binephrectomized rats (3.202 ng per ml of blood plus or minus 0.330, p smaller than 0.025). The biologically active substances detected in 25 ml of blood collected during expansion, while the assay organs showed a prostaglandin-like activity, were found to have the chromatographic behaviour and the bioassay properties of PGA, PGE and PGF series. A great quantity of the biologically active substances, having the chromatographic behaviour and the bioassay properties of PGA, PGS and PGF was detected in the rat renal medulla. Sufficient quantities of the released prostaglandin-like substances could escape the pulmonary vascular bed in this species of animal. It was concluded that a great quantity of the released prostaglandin-like substances came from the kidney and their release by this particular mechanism suggested that they play an important homeostatic role on the blood pressure, blood volume, and sodium and water balance regulation."}
{"id": "PMID:1096033", "title": "[The inhibitory mechanism responsible for antigenic competition in the mouse, upon successive injections of 2 different antigens: the immunosuppressive function of T cells].", "content": "Antigenic competition cannot be obtained in congenital athymic Nude mice, whatever the dose of inhibitor Ag used and the free interval between inhibitory Ag injections and the Ag test. On the other hand, competition is normal and even increased, in thymectomised, adult Swiss mice, used 10 to 30 days after thymectomy. In normal mice, the inhibitory mechanism induced by a first thymodependent antigen, may also occur with regard to certain thymo-independent antigens and thus act on the B cells. The antigens may also serve as inhibitory antigens for thymodependent antigens if the free interval between the two injections is small. Injection of hydrocortisone (25 mg/kg) or ALS a short time before the inhibitory antigens, suppresses competition. On the other hand, methotrexate is without effect. Thus the inhibitory TD mechanism does not require mitoses (methotrexate) and depends on corticosensitive T cells which are, no doubt, recirculated (Anti-lymphocyte serum) with a life span greater than 30 days (adult thymectomy). The previous results were obtained when two antigens were administered by intraperitoneal route and the PF cells sought in the spleen. The same experiment was carried out, injecting two antigens subcutaneously, in the same limb, and counting the PF cells in the appropriate lymph node; it shows the adjuvant effect exerted by the first antigen on the response with regard to the second antigen. On the other hand, inhibition is produced if the first antigen is injected intraperitoneally or into another limb. One may thus distinguish an inhibitory effect or an adjuvant effect depending on the experimental conditions. The inhibitory effect seems to be related to the T cells with an immunosuppressive function.", "contents": "[The inhibitory mechanism responsible for antigenic competition in the mouse, upon successive injections of 2 different antigens: the immunosuppressive function of T cells]. Antigenic competition cannot be obtained in congenital athymic Nude mice, whatever the dose of inhibitor Ag used and the free interval between inhibitory Ag injections and the Ag test. On the other hand, competition is normal and even increased, in thymectomised, adult Swiss mice, used 10 to 30 days after thymectomy. In normal mice, the inhibitory mechanism induced by a first thymodependent antigen, may also occur with regard to certain thymo-independent antigens and thus act on the B cells. The antigens may also serve as inhibitory antigens for thymodependent antigens if the free interval between the two injections is small. Injection of hydrocortisone (25 mg/kg) or ALS a short time before the inhibitory antigens, suppresses competition. On the other hand, methotrexate is without effect. Thus the inhibitory TD mechanism does not require mitoses (methotrexate) and depends on corticosensitive T cells which are, no doubt, recirculated (Anti-lymphocyte serum) with a life span greater than 30 days (adult thymectomy). The previous results were obtained when two antigens were administered by intraperitoneal route and the PF cells sought in the spleen. The same experiment was carried out, injecting two antigens subcutaneously, in the same limb, and counting the PF cells in the appropriate lymph node; it shows the adjuvant effect exerted by the first antigen on the response with regard to the second antigen. On the other hand, inhibition is produced if the first antigen is injected intraperitoneally or into another limb. One may thus distinguish an inhibitory effect or an adjuvant effect depending on the experimental conditions. The inhibitory effect seems to be related to the T cells with an immunosuppressive function."}
{"id": "PMID:1096029", "title": "[A histologic study of Ceratophyllus consimilis Wagn. fleas infected by the agent of murine typhus (Salmonella typhimurium)].", "content": "Histological studies of C. consimilis infected with S. typhimurium have shown that the microbes are preserves along the whole extent of the alimentary canal, expecially in the midgut and pyloric portion. Bacteria are concentrated in the gastral cavity; in addition, they were found to penetrate the alimentary epithelium cells and pyloric valve tissue. The number of microbes and their distribution in the intestine undergo great variations that depends largely on the insects feeding rhythm. During bloodsucking a \"lavenment\" of the alimantary canal takes place; as a result, numerous microbes accumulated here are periodically excreted outside. The infection with Salmonella typhimurium is often accompanied by pathological changes in fleas tht is expressed in the destruction of the midgut epithelium and pyloric valve tissue.", "contents": "[A histologic study of Ceratophyllus consimilis Wagn. fleas infected by the agent of murine typhus (Salmonella typhimurium)]. Histological studies of C. consimilis infected with S. typhimurium have shown that the microbes are preserves along the whole extent of the alimentary canal, expecially in the midgut and pyloric portion. Bacteria are concentrated in the gastral cavity; in addition, they were found to penetrate the alimentary epithelium cells and pyloric valve tissue. The number of microbes and their distribution in the intestine undergo great variations that depends largely on the insects feeding rhythm. During bloodsucking a \"lavenment\" of the alimantary canal takes place; as a result, numerous microbes accumulated here are periodically excreted outside. The infection with Salmonella typhimurium is often accompanied by pathological changes in fleas tht is expressed in the destruction of the midgut epithelium and pyloric valve tissue."}
{"id": "PMID:1096034", "title": "[Cerebromeningeal cryptococcosis. Predisposing role of immunosuppressive therapy in patients with kidney transplants].", "content": "Disseminated cryptococcal infection is described in eight patients, seven of them with verified meningeal involvement. Six of the eight patients were recipients of a renal homograft and submitted to the classical immunosuppressive treatment. Consideration is given to predisposing factors and to problems in the clinical, biological and mycological diagnosis. Some comments are presented on the often disappointing results of antifungal therapy of cryptococcal meningitis.", "contents": "[Cerebromeningeal cryptococcosis. Predisposing role of immunosuppressive therapy in patients with kidney transplants]. Disseminated cryptococcal infection is described in eight patients, seven of them with verified meningeal involvement. Six of the eight patients were recipients of a renal homograft and submitted to the classical immunosuppressive treatment. Consideration is given to predisposing factors and to problems in the clinical, biological and mycological diagnosis. Some comments are presented on the often disappointing results of antifungal therapy of cryptococcal meningitis."}
{"id": "PMID:1096035", "title": "[Encephalitis and ornithosis].", "content": "Neuropathology of ornithosis encephalitis in an adult man is reported. Lesions were widespread with severe vascular congestion, hyaline thrombi, intense perivascular infiltration by mononucleated cells and microglial reaction in the parenchyma. No specific lesion of the neurons was found.", "contents": "[Encephalitis and ornithosis]. Neuropathology of ornithosis encephalitis in an adult man is reported. Lesions were widespread with severe vascular congestion, hyaline thrombi, intense perivascular infiltration by mononucleated cells and microglial reaction in the parenchyma. No specific lesion of the neurons was found."}
{"id": "PMID:1096036", "title": "[Influence of the daily ingestion of a total unsaponifiable extract from avocado and soy bean oils on cholesterol metabolism in the rat].", "content": "The effects of ingestion of an unsaponifiable avocado and soya extract (P.I.A.S.) on the rates of cholesterol turnover were studied in the Rat smaller than in toto greater than. The balance is the same as in control rats, but fecal and urinary excretions of cholesterol are well enhanced and transformation into bile acids is consequently reduced. The absorption coefficient of cholesterol is unchanged in spite of the presence of phytosterols in the extract. The extract having been said to have a beneficial effect on scleroderma a special study was undertaken of the skin. The GLC analysis of sterols show the very probable presence of phytosterols and a richer proportion of cholesterol, precursors particularly methostenol in the skin of rats fed on P.I.A.S. than in that of the controls. These modifications do not produce any variations in the rate of cholesterol synthesis as measured in vitro.", "contents": "[Influence of the daily ingestion of a total unsaponifiable extract from avocado and soy bean oils on cholesterol metabolism in the rat]. The effects of ingestion of an unsaponifiable avocado and soya extract (P.I.A.S.) on the rates of cholesterol turnover were studied in the Rat smaller than in toto greater than. The balance is the same as in control rats, but fecal and urinary excretions of cholesterol are well enhanced and transformation into bile acids is consequently reduced. The absorption coefficient of cholesterol is unchanged in spite of the presence of phytosterols in the extract. The extract having been said to have a beneficial effect on scleroderma a special study was undertaken of the skin. The GLC analysis of sterols show the very probable presence of phytosterols and a richer proportion of cholesterol, precursors particularly methostenol in the skin of rats fed on P.I.A.S. than in that of the controls. These modifications do not produce any variations in the rate of cholesterol synthesis as measured in vitro."}
{"id": "PMID:1096037", "title": "[Influence of environmental conditions on human hematologic constants].", "content": "A blood investigation was carried out on 3,030 volunteer blood donors and the results treated on a computer by multivariant analysis. The authors noted a depressor effect of the urban environment on the red and white cells, a rise in small lymphocytes and a reduction in monocytes in patients living in industrial areas. This stimulation of the lymphocyte system may be compared with the experimental results obtained by the same authors in animals.", "contents": "[Influence of environmental conditions on human hematologic constants]. A blood investigation was carried out on 3,030 volunteer blood donors and the results treated on a computer by multivariant analysis. The authors noted a depressor effect of the urban environment on the red and white cells, a rise in small lymphocytes and a reduction in monocytes in patients living in industrial areas. This stimulation of the lymphocyte system may be compared with the experimental results obtained by the same authors in animals."}
{"id": "PMID:1096038", "title": "A shadow-casting technique for the examination of the surface topography of cultured cells.", "content": "Modifications to a technique of shadowing cultured cells for examination by the light microscope are presented. These consist of using a low angle of incidence, rotating the specimen and employing aluminium as the shadowing material.", "contents": "A shadow-casting technique for the examination of the surface topography of cultured cells. Modifications to a technique of shadowing cultured cells for examination by the light microscope are presented. These consist of using a low angle of incidence, rotating the specimen and employing aluminium as the shadowing material."}
{"id": "PMID:1096060", "title": "\"Car. factor\" deficiency revisited.", "content": "Three members of a Virginia family with a bleeding disorder were found to have a serum defect in thromboplastin generation similar to the previously reported \"Car. factor\" deficiency. Sera from three members of the original Car. family did not correct the defect of affected members of the Virginia family. Partial thromboplastin times of Car. deficient individuals and affected members of the Virginia family were normal. Although correction with normal serum is attained in vitro, the serum defect persisted after infusion of fresh frozen plasma. Platelet function studies of the Virginia family revealed less than 30% aggregation after the addition of exogenous ADP and disaggregation within 2 min. Evaluation of children with Noonan's syndrome, albinism, and \"Portsmouth\" syndrome showed coexistent platelet aggregation defects and nonspecific serum defects.", "contents": "\"Car. factor\" deficiency revisited. Three members of a Virginia family with a bleeding disorder were found to have a serum defect in thromboplastin generation similar to the previously reported \"Car. factor\" deficiency. Sera from three members of the original Car. family did not correct the defect of affected members of the Virginia family. Partial thromboplastin times of Car. deficient individuals and affected members of the Virginia family were normal. Although correction with normal serum is attained in vitro, the serum defect persisted after infusion of fresh frozen plasma. Platelet function studies of the Virginia family revealed less than 30% aggregation after the addition of exogenous ADP and disaggregation within 2 min. Evaluation of children with Noonan's syndrome, albinism, and \"Portsmouth\" syndrome showed coexistent platelet aggregation defects and nonspecific serum defects."}
{"id": "PMID:1096061", "title": "Lamb fetal pulmonary fluid. I. Validation and significance of method for determination of volume and volume change.", "content": "An indicator-dilution method is described for measuring fetal pulmonary fluid, FPF, volume (Ve), and volume change with time (Vs) in the lamb fetus in utero. The indicator, albumin, which is the predominant protein of normal FPF, was administered directly to FPF in the form of radio-iodinated human serum albumin (RISA). We have shown that (1) RISA is distributed homogeneously throughout the FPF compartment, (2) it is not altered within FPF, (3) it does not alter the functional characteristics of FPF, and (4) it remains within the FPF compartment throughout the period of measurement. Ve varies directly with the weight of the fetus so that Ve per kilogram was 31.6-35.6 ml/kg in four of five fetuses. The Ve per kilogram was lower in one fetus at the start of the experiment but increased rapidly to 29.0 ml/kg within 45 min. Thus the relaxation volume of the fetus in utero is in the range of functional residual capacity (FRC) and thoracic gas volume of the air-breathing neonate, which indicates that the pulmonary transformation at birth is, in essence, an isovolumic change in state wherein the fetal liquid-lung becomes the neonatal air-lung. The rate at which FPF is formed (Vs) is about 1.5 ml/hr-kg; however, the rate may change in so far as both rapid increases and rapid decreases of FPF volume were observed. When breathing movements were induced in the fetus by stimulation of the sciatic nerve, FPF volume decreased rapidly (Vs equals -3.0 ml/hr-kg), which indicates that negative intrathoracic pressures promote its resorption. When FPF volume fell transiently or was low initially, there was subsequent rapid restoration of volume to the range of anticipated FRC.", "contents": "Lamb fetal pulmonary fluid. I. Validation and significance of method for determination of volume and volume change. An indicator-dilution method is described for measuring fetal pulmonary fluid, FPF, volume (Ve), and volume change with time (Vs) in the lamb fetus in utero. The indicator, albumin, which is the predominant protein of normal FPF, was administered directly to FPF in the form of radio-iodinated human serum albumin (RISA). We have shown that (1) RISA is distributed homogeneously throughout the FPF compartment, (2) it is not altered within FPF, (3) it does not alter the functional characteristics of FPF, and (4) it remains within the FPF compartment throughout the period of measurement. Ve varies directly with the weight of the fetus so that Ve per kilogram was 31.6-35.6 ml/kg in four of five fetuses. The Ve per kilogram was lower in one fetus at the start of the experiment but increased rapidly to 29.0 ml/kg within 45 min. Thus the relaxation volume of the fetus in utero is in the range of functional residual capacity (FRC) and thoracic gas volume of the air-breathing neonate, which indicates that the pulmonary transformation at birth is, in essence, an isovolumic change in state wherein the fetal liquid-lung becomes the neonatal air-lung. The rate at which FPF is formed (Vs) is about 1.5 ml/hr-kg; however, the rate may change in so far as both rapid increases and rapid decreases of FPF volume were observed. When breathing movements were induced in the fetus by stimulation of the sciatic nerve, FPF volume decreased rapidly (Vs equals -3.0 ml/hr-kg), which indicates that negative intrathoracic pressures promote its resorption. When FPF volume fell transiently or was low initially, there was subsequent rapid restoration of volume to the range of anticipated FRC."}
{"id": "PMID:1096062", "title": "Lamb fetal pulmonary fluid. II. Fate of phosphatidylcholine.", "content": "Using the radio-iodinated human serum albumin ([131-I]-RISA) dilution method to measure lamb fetal pulmonary fluid (FPF) volume, we followed the disappearance of protein-complexed, 14-C-labeled phosphatidylcholine ([14-C]PC) during the first 90 min after its injection into FPF. The FPF samples were analyzed for total lipid 14-C activity and for distribution of 14-C in PC, other phospholipids (PL), fatty acids (FA), and neutral lipids (NL). For most sampling periods ascending aortic (AAo) and right atrial (RA) blood samples were obtained simultaneously with FPF and serum was analyzed for total lipid 14-C activity and for distribution of 14-C in total PL, FA, and NL. These studies indicate that (1) PC is cleared rapidly from FPF with an estimated half-time of 15-57 min; (2) FPF-PC may be metabolized to lyso-PC and FA within the fluid itself; and (3) FA derived from PFP-PC enter the pulmonary circulation, thus establishing a pulmonary arteriovenous FA gradient. The possible sites at which PC may be cleared from FPF are considered.", "contents": "Lamb fetal pulmonary fluid. II. Fate of phosphatidylcholine. Using the radio-iodinated human serum albumin ([131-I]-RISA) dilution method to measure lamb fetal pulmonary fluid (FPF) volume, we followed the disappearance of protein-complexed, 14-C-labeled phosphatidylcholine ([14-C]PC) during the first 90 min after its injection into FPF. The FPF samples were analyzed for total lipid 14-C activity and for distribution of 14-C in PC, other phospholipids (PL), fatty acids (FA), and neutral lipids (NL). For most sampling periods ascending aortic (AAo) and right atrial (RA) blood samples were obtained simultaneously with FPF and serum was analyzed for total lipid 14-C activity and for distribution of 14-C in total PL, FA, and NL. These studies indicate that (1) PC is cleared rapidly from FPF with an estimated half-time of 15-57 min; (2) FPF-PC may be metabolized to lyso-PC and FA within the fluid itself; and (3) FA derived from PFP-PC enter the pulmonary circulation, thus establishing a pulmonary arteriovenous FA gradient. The possible sites at which PC may be cleared from FPF are considered."}
{"id": "PMID:1096064", "title": "Neonatal paroxysmal supraventricular tachycardia with hydrops.", "content": "A critically ill infant with paroxysmal supraventricular tachycardia and hydrops fetalis responded well to aggressive management. Care must be taken to avoid digitalis toxicity. Procaine amide or quinidine are effective alternate therapies.", "contents": "Neonatal paroxysmal supraventricular tachycardia with hydrops. A critically ill infant with paroxysmal supraventricular tachycardia and hydrops fetalis responded well to aggressive management. Care must be taken to avoid digitalis toxicity. Procaine amide or quinidine are effective alternate therapies."}
{"id": "PMID:1096075", "title": "[Immunofluorescence technique in diagnosis of trichinellosis in swine. I. Investigations with the tube test (author's transl)].", "content": "Experiments have been made with serological demonstration of Trichinella spiralis infections in swine. The technique applied was the indirect immunofluorescence tube test with whole larvae of T. spiralis as antigen. The blood samples tested were taken from groups of SPF swine inoculated with, respectively, 50, 150, 500 and 1500 Trichinella larvae. In animals inoculated with at least 150 larvae, antibodies could be demonstrated 3-5 weeks p.i. until the investigation was finished 6-7 months later. The technique applied thus appears to represent a reasonably sensitive and reliable method for demonstrating infections with T. spiralis in swine.", "contents": "[Immunofluorescence technique in diagnosis of trichinellosis in swine. I. Investigations with the tube test (author's transl)]. Experiments have been made with serological demonstration of Trichinella spiralis infections in swine. The technique applied was the indirect immunofluorescence tube test with whole larvae of T. spiralis as antigen. The blood samples tested were taken from groups of SPF swine inoculated with, respectively, 50, 150, 500 and 1500 Trichinella larvae. In animals inoculated with at least 150 larvae, antibodies could be demonstrated 3-5 weeks p.i. until the investigation was finished 6-7 months later. The technique applied thus appears to represent a reasonably sensitive and reliable method for demonstrating infections with T. spiralis in swine."}
{"id": "PMID:1096076", "title": "[Immunofluorescence technique in diagnosis of trichinellosis in swine. II. Comparative investigations with the tube test and the cryostate method (author's transl)].", "content": "A series of comparative IF tests have been made on serum samples from 9 SPF pigs experimentally infected with Trichinella spiralis (50-500 larvae per animal). The techniques applied included a tube test (antigen: whole Trichinella larvae), and a cryostat test (antigen: sections of Trichinella larvae). With the cryostat test a positive reaction was recorded in 2 animals already on the 6th day and in 5 animals on the 13th day p.i., while with the tube test such reaction was not demonstrated until the 27th day p.i. (2 animals). A potential risk of cross-reaction of unknown origin seems, however, to limit the application of the cryostat test to SPF- pigs.", "contents": "[Immunofluorescence technique in diagnosis of trichinellosis in swine. II. Comparative investigations with the tube test and the cryostate method (author's transl)]. A series of comparative IF tests have been made on serum samples from 9 SPF pigs experimentally infected with Trichinella spiralis (50-500 larvae per animal). The techniques applied included a tube test (antigen: whole Trichinella larvae), and a cryostat test (antigen: sections of Trichinella larvae). With the cryostat test a positive reaction was recorded in 2 animals already on the 6th day and in 5 animals on the 13th day p.i., while with the tube test such reaction was not demonstrated until the 27th day p.i. (2 animals). A potential risk of cross-reaction of unknown origin seems, however, to limit the application of the cryostat test to SPF- pigs."}
{"id": "PMID:1096079", "title": "[Functional scintigraphy of the right heart].", "content": "After intravenous injection of 99m-Tc-labelled HSA-microspheres indicator dilution curves of vena cava superior, total right heart and lungs are recorded, using a Nuclear Chicago Pho Gamma II camera and a Hewlett Packard 5407 A computer system. A method is described, which permits the determination of the distribution of transit times, the mean transit time and the ejection fraction of the right heart on the basis of these three curves. The usefulness of the method for cardiac investigations is discussed.", "contents": "[Functional scintigraphy of the right heart]. After intravenous injection of 99m-Tc-labelled HSA-microspheres indicator dilution curves of vena cava superior, total right heart and lungs are recorded, using a Nuclear Chicago Pho Gamma II camera and a Hewlett Packard 5407 A computer system. A method is described, which permits the determination of the distribution of transit times, the mean transit time and the ejection fraction of the right heart on the basis of these three curves. The usefulness of the method for cardiac investigations is discussed."}
{"id": "PMID:1096080", "title": "Enzymic in vitro repair and chemical nature of DNA chain breaks induced by incorporated phosphorus-32P decay.", "content": "In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase treatment.", "contents": "Enzymic in vitro repair and chemical nature of DNA chain breaks induced by incorporated phosphorus-32P decay. In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase treatment."}
{"id": "PMID:1096081", "title": "The preparation of 5-cyanouracil and 5-cyano-2'-deoxyuridine from the corresponding 5-iodo derivative and cuprous cyanide.", "content": "5-Cyanouracil has been prepared in high yield from cuprous cyanide and 5-iodouracil. The deoxynucleoside has been similarly prepared form 5-iodo-2'-deoxyuridine and this has enabled these compounds to be labelled with (14-C) cyanide. Attempts have been made to incorporate 5-cyanouracil into Escherichia coli 15T and into Mycoplasma mycoides var. capri DNA under conditions in which several other 5-substituted uracils have been incorporated, but without success. Similarly 5-cyano-2'-deoxyuridine could not be incorporated into the DNA of T3 phage under conditions in which 5-bromo-2'-deoxyuridine is easily incorporated. These results suggest that the criteria for a 5-substituted uracil to be incorporated into DNA in vivo depends on some factor other than the size of the substituent.", "contents": "The preparation of 5-cyanouracil and 5-cyano-2'-deoxyuridine from the corresponding 5-iodo derivative and cuprous cyanide. 5-Cyanouracil has been prepared in high yield from cuprous cyanide and 5-iodouracil. The deoxynucleoside has been similarly prepared form 5-iodo-2'-deoxyuridine and this has enabled these compounds to be labelled with (14-C) cyanide. Attempts have been made to incorporate 5-cyanouracil into Escherichia coli 15T and into Mycoplasma mycoides var. capri DNA under conditions in which several other 5-substituted uracils have been incorporated, but without success. Similarly 5-cyano-2'-deoxyuridine could not be incorporated into the DNA of T3 phage under conditions in which 5-bromo-2'-deoxyuridine is easily incorporated. These results suggest that the criteria for a 5-substituted uracil to be incorporated into DNA in vivo depends on some factor other than the size of the substituent."}
{"id": "PMID:1096082", "title": "A study of the interaction of histones with DNA using isopycnic centrifugation in metrizamide gradients.", "content": "Isopycnic sedimentation in metrizamide gradients has shown that mouse-liver histones bind co-operatively to both homologous and bacterial DNA's. However, at low input ratios of histone to DNA, two types of stable complex are formed, depending on the histone concentration. One complex contains half as much histone as DNA while the other contains approximately equal amounts of histone and DNA. At high input ratios of histone to DNA extra histone is bound giving complexes containing up to twice as much histone as DNA. Poly-L-lysine and protamine were also found to bind co-operatively to DNA.", "contents": "A study of the interaction of histones with DNA using isopycnic centrifugation in metrizamide gradients. Isopycnic sedimentation in metrizamide gradients has shown that mouse-liver histones bind co-operatively to both homologous and bacterial DNA's. However, at low input ratios of histone to DNA, two types of stable complex are formed, depending on the histone concentration. One complex contains half as much histone as DNA while the other contains approximately equal amounts of histone and DNA. At high input ratios of histone to DNA extra histone is bound giving complexes containing up to twice as much histone as DNA. Poly-L-lysine and protamine were also found to bind co-operatively to DNA."}
{"id": "PMID:1096083", "title": "Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae.", "content": "Increasing amounts of mitochondrial [32P] tRNA (4S fraction), were hybridized with mitochondrial DNA OF Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G+C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about 1T per sequence), U, hU are present in mitochondrial tRNA. Some sequence may begin with pG.", "contents": "Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae. Increasing amounts of mitochondrial [32P] tRNA (4S fraction), were hybridized with mitochondrial DNA OF Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G+C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about 1T per sequence), U, hU are present in mitochondrial tRNA. Some sequence may begin with pG."}
{"id": "PMID:1096084", "title": "An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.", "content": "An improved method for the rapid separation of aminoacyl-tRNA from tRNA by chromatography on dihydroxyboryl-substituted cellulose has been developed. The method relies on the selective binding of unacylated tRNA to the cell cellulose support containing dihydroxyboryl groups. This binding is the result of complex formation between the cis-diol group of the 3'-terminal ribose in tRNA and the dihydroxyboryl groups immobilized on the resin. Aminoacyl-tRNA cannot undergo borate complex formation and is not retained on the resin. The separation is carried out at near neutral pH values ensuring stability of the aminoacyl ester linkage. The aminoacyl-tRNAs are obtained in very high purity. Aminoacyl-tRNA species containing the modified nucleoside Q are also retained on dihydroxyboryl cellulose. Conditions for isolating all Q base containing tRNA species from unfractionated tRNA are described.", "contents": "An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose. An improved method for the rapid separation of aminoacyl-tRNA from tRNA by chromatography on dihydroxyboryl-substituted cellulose has been developed. The method relies on the selective binding of unacylated tRNA to the cell cellulose support containing dihydroxyboryl groups. This binding is the result of complex formation between the cis-diol group of the 3'-terminal ribose in tRNA and the dihydroxyboryl groups immobilized on the resin. Aminoacyl-tRNA cannot undergo borate complex formation and is not retained on the resin. The separation is carried out at near neutral pH values ensuring stability of the aminoacyl ester linkage. The aminoacyl-tRNAs are obtained in very high purity. Aminoacyl-tRNA species containing the modified nucleoside Q are also retained on dihydroxyboryl cellulose. Conditions for isolating all Q base containing tRNA species from unfractionated tRNA are described."}
{"id": "PMID:1096085", "title": "Specific cleavage of tRNA by nuclease S1.", "content": "Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.", "contents": "Specific cleavage of tRNA by nuclease S1. Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA."}
{"id": "PMID:1096086", "title": "The characterisation of a fragment of ribosomal protein S4 that is protected against trypsin digestion by 16S ribosomal RNA of Escherichia coli.", "content": "After mild trypsin treatment of a complex of ribosomal protein S4 and 16S RNA of Escherichia coli, a large homogeneous fragment of the S4 protein was protected against digestion by its RNA binding site. This fragment was isolated and characterised for molecular weight. It was able to rebind specifically to 16S RNA. Preliminary results indicate that protected protein fragments can also be obtained from other proteins that complex specifically with 23S and 5S RNA.", "contents": "The characterisation of a fragment of ribosomal protein S4 that is protected against trypsin digestion by 16S ribosomal RNA of Escherichia coli. After mild trypsin treatment of a complex of ribosomal protein S4 and 16S RNA of Escherichia coli, a large homogeneous fragment of the S4 protein was protected against digestion by its RNA binding site. This fragment was isolated and characterised for molecular weight. It was able to rebind specifically to 16S RNA. Preliminary results indicate that protected protein fragments can also be obtained from other proteins that complex specifically with 23S and 5S RNA."}
{"id": "PMID:1096087", "title": "Two transfer RNA (1-methylguanine) methylases from yeast.", "content": "Two distinct tRNA (m-1G) methylases have been found in the yeast Saccharomyces cerevisiae. They differ in their chromatographic properties on hydroxyapatite, in their response to spermine, and in their site specificity. Only one of the methylases is active against normal tRNA from Escherichia coli.", "contents": "Two transfer RNA (1-methylguanine) methylases from yeast. Two distinct tRNA (m-1G) methylases have been found in the yeast Saccharomyces cerevisiae. They differ in their chromatographic properties on hydroxyapatite, in their response to spermine, and in their site specificity. Only one of the methylases is active against normal tRNA from Escherichia coli."}
{"id": "PMID:1096100", "title": "The influence of anti-inflammatory drugs on the values of xylose test in man.", "content": "Anti-inflammatory drugs influence intestinal absorption. Acetylsalicylic acid, indomethacin, and PAS decrease significantly the values of xylose test in man. Paracetamol decreases the values of the test in two thirds of the subjects. Salicylamide, phenylbutazone, and sulfadimethoxine do not possess this action.", "contents": "The influence of anti-inflammatory drugs on the values of xylose test in man. Anti-inflammatory drugs influence intestinal absorption. Acetylsalicylic acid, indomethacin, and PAS decrease significantly the values of xylose test in man. Paracetamol decreases the values of the test in two thirds of the subjects. Salicylamide, phenylbutazone, and sulfadimethoxine do not possess this action."}
{"id": "PMID:1096102", "title": "Antimalarial compounds. XIV. New derivatives of 2-bromo-N,N-bis-(diethylaminoethyl)-4,5-dimethoxyanaline (RC 12).", "content": "The molecule of RC 12 which was found previously to exhibit antimalarial activity has been modified, by replacing groups in positions 1 and 2 by NHAr, S-Ar, NO2, sulfonamido and other substituents. The new derivatives (Table 1) were tested for toxicity and antimalarial activity agains Plasmodium berghei in mice (Table 2).", "contents": "Antimalarial compounds. XIV. New derivatives of 2-bromo-N,N-bis-(diethylaminoethyl)-4,5-dimethoxyanaline (RC 12). The molecule of RC 12 which was found previously to exhibit antimalarial activity has been modified, by replacing groups in positions 1 and 2 by NHAr, S-Ar, NO2, sulfonamido and other substituents. The new derivatives (Table 1) were tested for toxicity and antimalarial activity agains Plasmodium berghei in mice (Table 2)."}
{"id": "PMID:1096101", "title": "Antimalarial compounds. XIII. New derivatives of phenyl chloromethyl sulfone.", "content": "A series of chloromethylsulfonyl derivatives of amines, biguanides and amidineureas (Scheme 1) has been prepared and their antimalarial properties investigated.", "contents": "Antimalarial compounds. XIII. New derivatives of phenyl chloromethyl sulfone. A series of chloromethylsulfonyl derivatives of amines, biguanides and amidineureas (Scheme 1) has been prepared and their antimalarial properties investigated."}
{"id": "PMID:1096120", "title": "Prazosin in the treatment of hypertension.", "content": "In two double-blind, randomized cross-over comparison studies undertaken in elderly subjects, a new quinazoline derivative, prazosin, has been shown to be a safe and effective antihypertensive agent to which there is a progressive response over four- to six-week periods of oral administration at fixed dose-levels.", "contents": "Prazosin in the treatment of hypertension. In two double-blind, randomized cross-over comparison studies undertaken in elderly subjects, a new quinazoline derivative, prazosin, has been shown to be a safe and effective antihypertensive agent to which there is a progressive response over four- to six-week periods of oral administration at fixed dose-levels."}
{"id": "PMID:1096122", "title": "Topical treatment of dermatophytoses and candidoses.", "content": "In a double-blind trial clotrimazole cream was compared with tolnaftate cream in the treatment of dermatophytoses and with nystatin cream in the treatment of candidoses. Clotrimazole was shown to be effective against both dermatophytoses and candidoses and was as effective and acceptable as tolnaftate and nystatin creams in each condition.", "contents": "Topical treatment of dermatophytoses and candidoses. In a double-blind trial clotrimazole cream was compared with tolnaftate cream in the treatment of dermatophytoses and with nystatin cream in the treatment of candidoses. Clotrimazole was shown to be effective against both dermatophytoses and candidoses and was as effective and acceptable as tolnaftate and nystatin creams in each condition."}
{"id": "PMID:1096127", "title": "A comparison of intravenous aminophylline and salbutamol in the treatment of severe bronchospasm.", "content": "Intravenous salbutamool or aminophylline was administered under double-blind conditions to 20 asthmatic patients presenting with severe dyspnoea and needing urgent treatment with bronchodilators. Ten patients received salbutamol and ten patients received aminophylline. Objective measurements showing improvement were recorded in nineteen patients. A greater response was produced by salbutamol although this failed to reach statistical significance. Neither drug affected pulse or blood pressure. It is suggested that intravenous salbutamol is an effective bronchodialtor in the treatment of acute exacerbation of asthma or bronchitis.", "contents": "A comparison of intravenous aminophylline and salbutamol in the treatment of severe bronchospasm. Intravenous salbutamool or aminophylline was administered under double-blind conditions to 20 asthmatic patients presenting with severe dyspnoea and needing urgent treatment with bronchodilators. Ten patients received salbutamol and ten patients received aminophylline. Objective measurements showing improvement were recorded in nineteen patients. A greater response was produced by salbutamol although this failed to reach statistical significance. Neither drug affected pulse or blood pressure. It is suggested that intravenous salbutamol is an effective bronchodialtor in the treatment of acute exacerbation of asthma or bronchitis."}
{"id": "PMID:1096136", "title": "[Dwarfism and the Shereshevski\u012d-Turner syndrome in patients with congenital toxoplasmosis].", "content": "Fifty five patients with cerebral and hypophyseal nanism and Shereshevsky-Turner syndrome were examined for toxoplasmosis.  The diagnosis of toxoplasmosis was established on the basis of epidemiological and obstetrical anamnesis, clinical and roentgenological data and serological tests (complement fixation test in 2 modifications--by common and droplet method; precipitation test, fluorescent antibody test) and intradermal allergic test with toxoplasmin (ATT).  Of 48 patients with cerebral and hypophyseal nanism ATT proved to be positive in 17 (35.4 per cent); it was positive in 3 of 7 patients with Shereshevsky-Turner syndrome.  In some of the patients and their mothers serological tests for toxoplasmosis were also positive.  Thus, toxoplasmosis infection among the patients examined was 2.5-3 times more incident than the corresponding indices in healthy children.  It is supposed that there is pathology of hypothalamic regulation of hypophyseal functions in toxoplasmosis and nanism.  A possibility of pathology of the generative apparatus in maternal toxoplasmosis leading to development of chromosomal embryopathies could not be excluded.  The authors consider that further studies are necessary for ascertaining (in some of the cases) of the pathogenetic association between toxoplasmosis and growth and developmental disturbances.", "contents": "[Dwarfism and the Shereshevski\u012d-Turner syndrome in patients with congenital toxoplasmosis]. Fifty five patients with cerebral and hypophyseal nanism and Shereshevsky-Turner syndrome were examined for toxoplasmosis.  The diagnosis of toxoplasmosis was established on the basis of epidemiological and obstetrical anamnesis, clinical and roentgenological data and serological tests (complement fixation test in 2 modifications--by common and droplet method; precipitation test, fluorescent antibody test) and intradermal allergic test with toxoplasmin (ATT).  Of 48 patients with cerebral and hypophyseal nanism ATT proved to be positive in 17 (35.4 per cent); it was positive in 3 of 7 patients with Shereshevsky-Turner syndrome.  In some of the patients and their mothers serological tests for toxoplasmosis were also positive.  Thus, toxoplasmosis infection among the patients examined was 2.5-3 times more incident than the corresponding indices in healthy children.  It is supposed that there is pathology of hypothalamic regulation of hypophyseal functions in toxoplasmosis and nanism.  A possibility of pathology of the generative apparatus in maternal toxoplasmosis leading to development of chromosomal embryopathies could not be excluded.  The authors consider that further studies are necessary for ascertaining (in some of the cases) of the pathogenetic association between toxoplasmosis and growth and developmental disturbances."}
{"id": "PMID:1096137", "title": "[Simplified method of differential diagnosis of dwarfism].", "content": "On the basis of analysis (with the aid of the \"Minsk-22\" computer) of the data on the information character of 485 signs in hypophyseal, hypothalamic, thyrogenic, somatogenic nanism spondylo-epyphyseal dysplasia ans Shereshevsky-Turner's syndrome the authors selected 50 signs with the greatest diagnostic value.  These signs were assessed and graded by marks; a diagnostic table was elaborated with the aid of which a correct diagnosis with the probability of over 0.90 was established in 72 per cent of cases.", "contents": "[Simplified method of differential diagnosis of dwarfism]. On the basis of analysis (with the aid of the \"Minsk-22\" computer) of the data on the information character of 485 signs in hypophyseal, hypothalamic, thyrogenic, somatogenic nanism spondylo-epyphyseal dysplasia ans Shereshevsky-Turner's syndrome the authors selected 50 signs with the greatest diagnostic value.  These signs were assessed and graded by marks; a diagnostic table was elaborated with the aid of which a correct diagnosis with the probability of over 0.90 was established in 72 per cent of cases."}
{"id": "PMID:1096160", "title": "Separation of non-sperm components from seminal preparations and effect on the analysis of sperm proteins.", "content": "The presence of non-sperm components (NSC) in \"washed\" rabbit epididymal and ejaculated collections and their interference with the analysis of sperm proteins have been demonstrated. NSC were also found in ejaculates from normal and vasectomized humans and from vasectomized rabbits, and in epididymal preparations from guinea pigs. They comprise about 80% of the total number of particles in rabbit ejaculates and 35% in human ejaculates. The dry weight is approximately equally distributed in rabbit ejaculates between NSC and sperm. The majority of the LDH isozyme activity of the \"washed particles\" in an ejaculate were associated with the NSC and this study shows that \"washed particle\" preparations are not equivalent to sperm and that NSC must first be separated from the sperm themselves for studies of sperm proteins to be meaningful. A method for the separation of the NSC from the sperm by equilibrium sedimentation in gradients of sucrose-D20 is described.", "contents": "Separation of non-sperm components from seminal preparations and effect on the analysis of sperm proteins. The presence of non-sperm components (NSC) in \"washed\" rabbit epididymal and ejaculated collections and their interference with the analysis of sperm proteins have been demonstrated. NSC were also found in ejaculates from normal and vasectomized humans and from vasectomized rabbits, and in epididymal preparations from guinea pigs. They comprise about 80% of the total number of particles in rabbit ejaculates and 35% in human ejaculates. The dry weight is approximately equally distributed in rabbit ejaculates between NSC and sperm. The majority of the LDH isozyme activity of the \"washed particles\" in an ejaculate were associated with the NSC and this study shows that \"washed particle\" preparations are not equivalent to sperm and that NSC must first be separated from the sperm themselves for studies of sperm proteins to be meaningful. A method for the separation of the NSC from the sperm by equilibrium sedimentation in gradients of sucrose-D20 is described."}
{"id": "PMID:1096161", "title": "Factor(s) from Ehrlich Ascites cells responsible for delayed rejection of skin allografts in mice and its assay on lymphocytes in vitro.", "content": "Cell free Ehrlich ascitic fluid from tumor bearing mice and the nutrient medium from Ehrlich cells maintained in vitro contain a factor(s) which when transferred to normal mice delays rejection of skin allografts and enhances the growth of a syngeneic transplantable tumor. Both types of prepartions inhibit DNA synthesis in lymphocytes stimulated by PHA. These results suggest that Ehrlich tumor cells release a factor which impairs the normal lymphocytic response to allo and tumor antigens.", "contents": "Factor(s) from Ehrlich Ascites cells responsible for delayed rejection of skin allografts in mice and its assay on lymphocytes in vitro. Cell free Ehrlich ascitic fluid from tumor bearing mice and the nutrient medium from Ehrlich cells maintained in vitro contain a factor(s) which when transferred to normal mice delays rejection of skin allografts and enhances the growth of a syngeneic transplantable tumor. Both types of prepartions inhibit DNA synthesis in lymphocytes stimulated by PHA. These results suggest that Ehrlich tumor cells release a factor which impairs the normal lymphocytic response to allo and tumor antigens."}
{"id": "PMID:1096162", "title": "Inhibitory effect of synthetic polyribonucleotides on the primary in vitro immune response.", "content": "The synthetic double-stranded polyribonucleotides, poly (rA):poly (rU) and poly (rI):poly (rC), were shown to be potent inhibitors of the in vitro plaque-forming cell (PFC) response to a thymus-dependent (SRBC) and thymus-independent (E. coli 0127 LPS) antigen in mouse C57BL/6 spleen cell cultures. The same polynucleotides had no effect on the PFC response of nude (athymic) mouse spleen cells to E. coli 0127 LPS, suggesting that functional T lymphocytes are necessary for the inhibitory effect. Enhancement effects were modest and inconsistent in the cultures. Poly (rA) and poly (rU) were ineffective as inhibitors. The data indirectly suggest that the inhibition may be due to the early production of interferon by functional T lymphocytes.", "contents": "Inhibitory effect of synthetic polyribonucleotides on the primary in vitro immune response. The synthetic double-stranded polyribonucleotides, poly (rA):poly (rU) and poly (rI):poly (rC), were shown to be potent inhibitors of the in vitro plaque-forming cell (PFC) response to a thymus-dependent (SRBC) and thymus-independent (E. coli 0127 LPS) antigen in mouse C57BL/6 spleen cell cultures. The same polynucleotides had no effect on the PFC response of nude (athymic) mouse spleen cells to E. coli 0127 LPS, suggesting that functional T lymphocytes are necessary for the inhibitory effect. Enhancement effects were modest and inconsistent in the cultures. Poly (rA) and poly (rU) were ineffective as inhibitors. The data indirectly suggest that the inhibition may be due to the early production of interferon by functional T lymphocytes."}
{"id": "PMID:1096163", "title": "Actin in the cytoplasm of adrenocortical cells.", "content": "Rat or hamster adrenocortical cells contain a network of filaments mostly 40-80 A in diameter, which is particularly evident at the cell periphery. In frozen sections, adrenocortical cells fix (particularly at their periphery) anti-actin autoantibodies present in sera of patients with chronic aggressive hepatitis. It is concluded that the peripheral network of adrenocortical cells contains contractile proteins (at least actin) and the implication of such proteins in steroid secretion is discussed.", "contents": "Actin in the cytoplasm of adrenocortical cells. Rat or hamster adrenocortical cells contain a network of filaments mostly 40-80 A in diameter, which is particularly evident at the cell periphery. In frozen sections, adrenocortical cells fix (particularly at their periphery) anti-actin autoantibodies present in sera of patients with chronic aggressive hepatitis. It is concluded that the peripheral network of adrenocortical cells contains contractile proteins (at least actin) and the implication of such proteins in steroid secretion is discussed."}
{"id": "PMID:1096164", "title": "Blood pressure changes in mice after lethal staphylococcal infection and endotoxin challenge.", "content": "A radioisotope technique has been used to determine blood pressure changes in mice after lethal staphylococcal infection and after lethal endotoxin challenge. The method was verified by making simultaneous direct measurements in rats. Mice in both groups became hypotensive to a similar level (a fall 20-30 mm Hg). This tailcuff technique is simple and reliable but is dependent upon normal tail blood flow. Spurious low pressure readings are obtained in hypothermic or chilled mice because the tail is a major thermoregulator organ. These difficulties can be overcome by warming chilled or hypothermic mice.", "contents": "Blood pressure changes in mice after lethal staphylococcal infection and endotoxin challenge. A radioisotope technique has been used to determine blood pressure changes in mice after lethal staphylococcal infection and after lethal endotoxin challenge. The method was verified by making simultaneous direct measurements in rats. Mice in both groups became hypotensive to a similar level (a fall 20-30 mm Hg). This tailcuff technique is simple and reliable but is dependent upon normal tail blood flow. Spurious low pressure readings are obtained in hypothermic or chilled mice because the tail is a major thermoregulator organ. These difficulties can be overcome by warming chilled or hypothermic mice."}
{"id": "PMID:1096165", "title": "Inhibitors of dopamine-beta-hydroxylase in human plasma.", "content": "There are two types of endogenous inhibitors of DBH in human plasma. One is sensitive to copper, or N-ethyl-maleimide, and is dialyzable. The other is not sensitive to copper, maleimide, or dialysis. Both types can be counteracted by diluting the plasma, thus eliminating the need for inactivators during the assay of plasma.", "contents": "Inhibitors of dopamine-beta-hydroxylase in human plasma. There are two types of endogenous inhibitors of DBH in human plasma. One is sensitive to copper, or N-ethyl-maleimide, and is dialyzable. The other is not sensitive to copper, maleimide, or dialysis. Both types can be counteracted by diluting the plasma, thus eliminating the need for inactivators during the assay of plasma."}
{"id": "PMID:1096166", "title": "Decrease in ribosomal density of Proteus mirabilis exposed to subinhibitory concentrations of ampicillin or cephalothin.", "content": "The finding of reduced ribosomal densities at lower concentrations than those required to stop growth or cause visible defects in the cell wall is in contrast to the current view that the initial lesion produced by penicillins or cephalosporins is a defect in murein synthesis (11). This reduction in ribosomal density could be a primary or secondary effect and might be due to a decreased rate of growth, which has been shown to be associated with a lower ribosome frequency (12, 13) or to less stable ribosomes which disintegrated either spontaneously or as a result of the fixation procedures. The possibility that the decrease in ribosomal frequency was due to dilution (influx of liquid through a defective cell wall and/or cytoplasmic membrane, or efflux of ribosomes) remains, but no gross defect in cell wall was seen in more than 50 sections of cells showing reductions in ribosomal frequency. These findings suggest that an effect on ribosomes may antecede an effect on the cell wall.", "contents": "Decrease in ribosomal density of Proteus mirabilis exposed to subinhibitory concentrations of ampicillin or cephalothin. The finding of reduced ribosomal densities at lower concentrations than those required to stop growth or cause visible defects in the cell wall is in contrast to the current view that the initial lesion produced by penicillins or cephalosporins is a defect in murein synthesis (11). This reduction in ribosomal density could be a primary or secondary effect and might be due to a decreased rate of growth, which has been shown to be associated with a lower ribosome frequency (12, 13) or to less stable ribosomes which disintegrated either spontaneously or as a result of the fixation procedures. The possibility that the decrease in ribosomal frequency was due to dilution (influx of liquid through a defective cell wall and/or cytoplasmic membrane, or efflux of ribosomes) remains, but no gross defect in cell wall was seen in more than 50 sections of cells showing reductions in ribosomal frequency. These findings suggest that an effect on ribosomes may antecede an effect on the cell wall."}
{"id": "PMID:1096167", "title": "Endotoxin binding by charged and uncharged resins.", "content": "Cholestyramine (Dowex 1-X2), a strongly basic anion-exchange resin, has previously been shown to bind bacterial endotoxin, preventing both its toxicity and intestinal absorption. Because hemoperfusion through charged and uncharged resins is practical, a study was undertaken to test the endotoxin-binding characteristics of a number of resins. The resin to be tested was washed and swelled overnight, and 1 mg/ml of 51Cr-labeled endotoxin was added and the mixture, agitated and incubated at 37 degrees for a specific time period. In the Dowex 1 series, the 1-X2 was superior to the 1-X4 and 1-X8 in its ability to bind E. coli endotoxin, removing about 90% from solution in 15 min. Increasing mesh size seemed to offer more binding sites for each Dowex 1 resin. Activated charcoal adsorbed about 90% of the endotoxin also, but Amberlite XAD-2 showed little binding capacity. Injection of filtrate from unlabeled E. coli and S. typhosa resin-treated solution into rats, demonstrated that both Dowex 1-X2 and activated charcoal prevented the transaminase rise noted in animals injected with solutions not so treated. It is concluded that Dowex 1-X2 resin and activated charcoal efficiently remove endotoxin in vitro, and may offer a unique method for removing circulating endotoxin in vivo.", "contents": "Endotoxin binding by charged and uncharged resins. Cholestyramine (Dowex 1-X2), a strongly basic anion-exchange resin, has previously been shown to bind bacterial endotoxin, preventing both its toxicity and intestinal absorption. Because hemoperfusion through charged and uncharged resins is practical, a study was undertaken to test the endotoxin-binding characteristics of a number of resins. The resin to be tested was washed and swelled overnight, and 1 mg/ml of 51Cr-labeled endotoxin was added and the mixture, agitated and incubated at 37 degrees for a specific time period. In the Dowex 1 series, the 1-X2 was superior to the 1-X4 and 1-X8 in its ability to bind E. coli endotoxin, removing about 90% from solution in 15 min. Increasing mesh size seemed to offer more binding sites for each Dowex 1 resin. Activated charcoal adsorbed about 90% of the endotoxin also, but Amberlite XAD-2 showed little binding capacity. Injection of filtrate from unlabeled E. coli and S. typhosa resin-treated solution into rats, demonstrated that both Dowex 1-X2 and activated charcoal prevented the transaminase rise noted in animals injected with solutions not so treated. It is concluded that Dowex 1-X2 resin and activated charcoal efficiently remove endotoxin in vitro, and may offer a unique method for removing circulating endotoxin in vivo."}
{"id": "PMID:1096168", "title": "Luteinizing hormone and androgens in the bovine fetus after gonadotropin-releasing hormone.", "content": "After an intra-arterial injection of 10 mug GnRH into four bovine male fetuses, serum LH increased (P less than 0.01) 2-fold by 15 min and plateaued at approximately 11 ng/ml from 60 to 180 min. After GnRH in six female fetuses, LH increased (P less than 0.01) 7-fold by 15 min and plateaued at approximately 17 ng/ml from 60 to 180 min. Maternal serum LH was not significantly influenced (P greater than 0.05) by sex of fetus or administration of GnRH to the fetus. After GnRH in male fetuses, serum testosterone increased (P less than 0.05) and remained 2-fold greater than basal levels through 180 min. In contrast, serum testosterone from female fetuses averaged 370 +/- 60 pg/ml at injection and was unchanged after GnRH. Androgen synthesis was significantly increased during in vitro incubation of fetal testicular explants by the addition of LH. We conclude that GnRH causes release of LH from the bovine fetal pituitary as early as 120 days of age and that the testes respond with testosterone secretion after LH stimulation.", "contents": "Luteinizing hormone and androgens in the bovine fetus after gonadotropin-releasing hormone. After an intra-arterial injection of 10 mug GnRH into four bovine male fetuses, serum LH increased (P less than 0.01) 2-fold by 15 min and plateaued at approximately 11 ng/ml from 60 to 180 min. After GnRH in six female fetuses, LH increased (P less than 0.01) 7-fold by 15 min and plateaued at approximately 17 ng/ml from 60 to 180 min. Maternal serum LH was not significantly influenced (P greater than 0.05) by sex of fetus or administration of GnRH to the fetus. After GnRH in male fetuses, serum testosterone increased (P less than 0.05) and remained 2-fold greater than basal levels through 180 min. In contrast, serum testosterone from female fetuses averaged 370 +/- 60 pg/ml at injection and was unchanged after GnRH. Androgen synthesis was significantly increased during in vitro incubation of fetal testicular explants by the addition of LH. We conclude that GnRH causes release of LH from the bovine fetal pituitary as early as 120 days of age and that the testes respond with testosterone secretion after LH stimulation."}
{"id": "PMID:1096172", "title": "Marijuana influenced changes in GSR activation peaking during paired-associate learning.", "content": "Activation Peaking (AP) refers to a patterned physiological response occurring during learning. Marijuana has been found to interfere with both paired-associate learning and phasic GSR activity. Therefore, a study was performed to assess the effects of marijuana intoxication on paired-associate learning and concomitant GSR AP. Two marijuana usage categories were employed--light and heavy usage Ss. Within each category four groups were run in a design to test state-dependent effects. Each S was seen twice with a seven-day inter-session interval. The groups were P-P, P-M, M-M and P-M with P equals placebo and M equals 14 mg delta-9 THC. At each sessions S learned a nine-word paired-associate list to a criterion of one correct recitation, and then received 100 percent overlearning. No usage or group differences were found in level of basal conductance, except lights showed habituation over sessions and heavies did not. Magnitude of phasic GSR activation, aligned for AP, was significantly reduced for both heavy usage and marijuana intoxicated Ss. Also, only on placebo days was an AP effect evident. The results were discussed in terms of marijuana's effects on learning and physiology with emphasis on possible mechanisms of action.", "contents": "Marijuana influenced changes in GSR activation peaking during paired-associate learning. Activation Peaking (AP) refers to a patterned physiological response occurring during learning. Marijuana has been found to interfere with both paired-associate learning and phasic GSR activity. Therefore, a study was performed to assess the effects of marijuana intoxication on paired-associate learning and concomitant GSR AP. Two marijuana usage categories were employed--light and heavy usage Ss. Within each category four groups were run in a design to test state-dependent effects. Each S was seen twice with a seven-day inter-session interval. The groups were P-P, P-M, M-M and P-M with P equals placebo and M equals 14 mg delta-9 THC. At each sessions S learned a nine-word paired-associate list to a criterion of one correct recitation, and then received 100 percent overlearning. No usage or group differences were found in level of basal conductance, except lights showed habituation over sessions and heavies did not. Magnitude of phasic GSR activation, aligned for AP, was significantly reduced for both heavy usage and marijuana intoxicated Ss. Also, only on placebo days was an AP effect evident. The results were discussed in terms of marijuana's effects on learning and physiology with emphasis on possible mechanisms of action."}
{"id": "PMID:1096173", "title": "Intracranial injection of drugs: comparison of diffusion of 6-OHDA and guanethidine.", "content": "Marked differences in extent of diffusion have been shown with the fluorescence histochemical method between guanethidine and 6-OHDA(64 mug in 2 mul) when injected acutely or chronically into the lateral hypothalamus, the substantia nigra or the amygdala of the rat brain. Cannulation damage up to 1 mm in diameter and attributed to the implantation of cannulae and placebo injection was observed. A further area of generalized damage occurred following the injection of drugs and was far greater for 6-OHDA (2 mm) than for guanethidien (0.3 mm). Guanethidine, but not 6-OHDA, caused specific damage to catecholamine-containing nerurons up to a distance of at least 3 mm and more from the cannula tip. These striking differences between the effects of intracranial injection of 6-OHDA and guanethidine are discussed in terms of the uptake and degradation of the two drugs and the anatomical features of the injection site; they are not explicable in terms of experimental conditions such as concentration, volume of injection, molecular weight or lipid solubility. The different patterns of damage would not easily be distinguished by biochemical analyses and the catecholamine specificity of 6-OHDA in studies of the central nervous system must be seriously questioned. Vascularization of chronically implanted cannula tracks and the presence of anatomical diffusion barriers are also discussed in relation to the diffusion of drugs injected intracranially.", "contents": "Intracranial injection of drugs: comparison of diffusion of 6-OHDA and guanethidine. Marked differences in extent of diffusion have been shown with the fluorescence histochemical method between guanethidine and 6-OHDA(64 mug in 2 mul) when injected acutely or chronically into the lateral hypothalamus, the substantia nigra or the amygdala of the rat brain. Cannulation damage up to 1 mm in diameter and attributed to the implantation of cannulae and placebo injection was observed. A further area of generalized damage occurred following the injection of drugs and was far greater for 6-OHDA (2 mm) than for guanethidien (0.3 mm). Guanethidine, but not 6-OHDA, caused specific damage to catecholamine-containing nerurons up to a distance of at least 3 mm and more from the cannula tip. These striking differences between the effects of intracranial injection of 6-OHDA and guanethidine are discussed in terms of the uptake and degradation of the two drugs and the anatomical features of the injection site; they are not explicable in terms of experimental conditions such as concentration, volume of injection, molecular weight or lipid solubility. The different patterns of damage would not easily be distinguished by biochemical analyses and the catecholamine specificity of 6-OHDA in studies of the central nervous system must be seriously questioned. Vascularization of chronically implanted cannula tracks and the presence of anatomical diffusion barriers are also discussed in relation to the diffusion of drugs injected intracranially."}
{"id": "PMID:1096180", "title": "Pulmonary problems in Duchenne muscular dystrophy. Diagnosis, prophylaxis, and treatment.", "content": "A number of pulmonary problems may complicate muscular dystrophy. Respiratory function, however, can be increased by instruction in diaphragmatic breathing. When patients effectively use their diaphragms during respiration, less muscular work is required for adequate ventilation. Additionally, assistive techniques such as postural drainage, chest percussion and vibration, and IPPB contribute to an ongoing program of respiratory care. Ten children with Duchenne muscular dystrophy underwent pulmonary function studies before, during, and after a three-month period of such respiratory therapy. Comparison of respiratory function before and after treatment indicates that such treatment is useful in increasing pulmonary function. The major goal of pulmonary care in the treatment of the patient with muscular dystrophy is the maintenance of respiratory homeostasis through  beathing exercises and training in the use of various breathing aids, thus enabling the patient to lead as active and comfortable a life as possible within the limits imposed by his evolving respiratory problem.", "contents": "Pulmonary problems in Duchenne muscular dystrophy. Diagnosis, prophylaxis, and treatment. A number of pulmonary problems may complicate muscular dystrophy. Respiratory function, however, can be increased by instruction in diaphragmatic breathing. When patients effectively use their diaphragms during respiration, less muscular work is required for adequate ventilation. Additionally, assistive techniques such as postural drainage, chest percussion and vibration, and IPPB contribute to an ongoing program of respiratory care. Ten children with Duchenne muscular dystrophy underwent pulmonary function studies before, during, and after a three-month period of such respiratory therapy. Comparison of respiratory function before and after treatment indicates that such treatment is useful in increasing pulmonary function. The major goal of pulmonary care in the treatment of the patient with muscular dystrophy is the maintenance of respiratory homeostasis through  beathing exercises and training in the use of various breathing aids, thus enabling the patient to lead as active and comfortable a life as possible within the limits imposed by his evolving respiratory problem."}
{"id": "PMID:1096174", "title": "Effects of chronic intracranial injection of low and high concentrations of guanethidine in the rat.", "content": "Low (64 mug in 2 mul) or high 320-1280 mug in 2 mul) doses of guanethidine sulphate were injected daily for up to 19 days into the lateral hypothalamus, substantia nigra, locus coeruleus, dorsal raphe nucleus, or amygdala region of the rat brain. Effects on monoamine-containing neurons were determined using fluorescence histochemistry. The noradrenergic terminals of the hypothalamus were depleted over a diameter of 7 mm by both low and high doses of guanethidine whereas, even with high doses, the dopaminergic terminals of the median eminence, amygdala and acudate nucleus were only partially depleted. Fluorescence levels of dopaminergic cell bodies of the sub stantis nigra and 5HT-containing cell bodies of the dorsal raphe nucleus were unaltered by low doses of guanethidine. Low doses of guanethidine did not affect the fluorescence of the noradrenergic cell bodies of the locus coeruleus, however high doses caused a substantial reduction in fluorescence levels. Normal levels of fluorescence were observed in all catecholamine-containing neurons within 14 days from cessation of injections. Thus, the xon retraction and eventual degeneration of peripheral sympathetic adrenergic neurons, which occurs as a result of chronic intraperitoneal injections of guanethidine does not occur with the catecholamine-containing neurons in the central nervous system. The rapid recovery of centrat catecholamine-containing neurons is remarkable in view of the extensive areas of brain damage produced by chronic injection of such high concentrations of drug. Fluorescence in peripheral adrenergic nerves was unaffected by chronic injection of guanethidine into the lateral hypothalamus but adhesions of some internal organs were observed. Blood vessels in the vicinity of the cannula were heavily reinnervated by fluorescent fibres probably arising from intracranial catecholamine-containing neurons. Some of the advantages of intracranial injection of guanethidine compared to 6-hydroxydopamine for behavioral experiments are discussed.", "contents": "Effects of chronic intracranial injection of low and high concentrations of guanethidine in the rat. Low (64 mug in 2 mul) or high 320-1280 mug in 2 mul) doses of guanethidine sulphate were injected daily for up to 19 days into the lateral hypothalamus, substantia nigra, locus coeruleus, dorsal raphe nucleus, or amygdala region of the rat brain. Effects on monoamine-containing neurons were determined using fluorescence histochemistry. The noradrenergic terminals of the hypothalamus were depleted over a diameter of 7 mm by both low and high doses of guanethidine whereas, even with high doses, the dopaminergic terminals of the median eminence, amygdala and acudate nucleus were only partially depleted. Fluorescence levels of dopaminergic cell bodies of the sub stantis nigra and 5HT-containing cell bodies of the dorsal raphe nucleus were unaltered by low doses of guanethidine. Low doses of guanethidine did not affect the fluorescence of the noradrenergic cell bodies of the locus coeruleus, however high doses caused a substantial reduction in fluorescence levels. Normal levels of fluorescence were observed in all catecholamine-containing neurons within 14 days from cessation of injections. Thus, the xon retraction and eventual degeneration of peripheral sympathetic adrenergic neurons, which occurs as a result of chronic intraperitoneal injections of guanethidine does not occur with the catecholamine-containing neurons in the central nervous system. The rapid recovery of centrat catecholamine-containing neurons is remarkable in view of the extensive areas of brain damage produced by chronic injection of such high concentrations of drug. Fluorescence in peripheral adrenergic nerves was unaffected by chronic injection of guanethidine into the lateral hypothalamus but adhesions of some internal organs were observed. Blood vessels in the vicinity of the cannula were heavily reinnervated by fluorescent fibres probably arising from intracranial catecholamine-containing neurons. Some of the advantages of intracranial injection of guanethidine compared to 6-hydroxydopamine for behavioral experiments are discussed."}
{"id": "PMID:1096181", "title": "Atrial receptors and renal function.", "content": "The hypothesis that receptors in the heart or pulmonary vasculature initiate a reflex that influences urine flow was derived from experiments designed to evaluate the effect of mechanical ventilation on renal function.  These experiments indicated that urine flow usually decreases during positive-pressure breathing and usually increases during negative-pressure breathing.  It was surmised that impulses from certain cardiopulmonary receptors affect the secretion of ADH, which in turn influences urine flow.  A subsequent investigation appeared to localize the pertinent receptors to the left atrium, but the results of this particular investigation were influenced by several complication factors that have not been widely appreciated.  The apparent localization of volume-regulating recpetors to the left atrium and the accumulating evidence that atrial receptors do respond to changes in atrial pressure or atrial volume triggered a myriad of further studies on the function of left receptors.  Nearly all these studies employed indirect techniques that produced changes in systemic and pulmonary hemodynamics in addition to changes in left atrial pressure.  Nevertheless, it often was assumed that if changes in left pressure were produced, any concomitant changes in circulating ADH or in urine flow were attributable to a reflex elicited from atrial receptors.  Mush of the data obtained were interpreted as being compatible with the elft atrial volume-receptor hypothesis, but very liggle of the data pertained to left atrial receptors specifically.", "contents": "Atrial receptors and renal function. The hypothesis that receptors in the heart or pulmonary vasculature initiate a reflex that influences urine flow was derived from experiments designed to evaluate the effect of mechanical ventilation on renal function.  These experiments indicated that urine flow usually decreases during positive-pressure breathing and usually increases during negative-pressure breathing.  It was surmised that impulses from certain cardiopulmonary receptors affect the secretion of ADH, which in turn influences urine flow.  A subsequent investigation appeared to localize the pertinent receptors to the left atrium, but the results of this particular investigation were influenced by several complication factors that have not been widely appreciated.  The apparent localization of volume-regulating recpetors to the left atrium and the accumulating evidence that atrial receptors do respond to changes in atrial pressure or atrial volume triggered a myriad of further studies on the function of left receptors.  Nearly all these studies employed indirect techniques that produced changes in systemic and pulmonary hemodynamics in addition to changes in left atrial pressure.  Nevertheless, it often was assumed that if changes in left pressure were produced, any concomitant changes in circulating ADH or in urine flow were attributable to a reflex elicited from atrial receptors.  Mush of the data obtained were interpreted as being compatible with the elft atrial volume-receptor hypothesis, but very liggle of the data pertained to left atrial receptors specifically."}
{"id": "PMID:1096175", "title": "Neurological and behavioral toxicity of kryptopyrrole in the rat.", "content": "Ten rats were given 9.1 to 82 mg/kg of 2,4-dimethyl-3-ethylpyrrole (kryptopyrrole) and the behavioral and electroencephalographic effects were studied. Kryptopyrrole was found to decrease EEG voltage, disrupt synchronization and induce abnormal spiking at a variety of cortical sites. Intermittent periods of low frequency hypersynchronous EEG activity was consistently elicited by kryptopyrrole. These waves bear a resemblance to the hypersynchronous EEG patterns associated with hallucinatory agents such as LSD-25. Marked behavioral alterations were observed following the initial injection including ataxia, hyperventilation, locomotor depression and catelepsy. Kryptopyrrole causes major central nervous system dysfunction, and these findings are discussed in the context of a drug-induced model of psychoses.", "contents": "Neurological and behavioral toxicity of kryptopyrrole in the rat. Ten rats were given 9.1 to 82 mg/kg of 2,4-dimethyl-3-ethylpyrrole (kryptopyrrole) and the behavioral and electroencephalographic effects were studied. Kryptopyrrole was found to decrease EEG voltage, disrupt synchronization and induce abnormal spiking at a variety of cortical sites. Intermittent periods of low frequency hypersynchronous EEG activity was consistently elicited by kryptopyrrole. These waves bear a resemblance to the hypersynchronous EEG patterns associated with hallucinatory agents such as LSD-25. Marked behavioral alterations were observed following the initial injection including ataxia, hyperventilation, locomotor depression and catelepsy. Kryptopyrrole causes major central nervous system dysfunction, and these findings are discussed in the context of a drug-induced model of psychoses."}
{"id": "PMID:1096176", "title": "Discriminated escape learning and response to electric shock after 6-hydroxydopamine lesions of the nigro-neostriatal dopaminergic projection.", "content": "In an earlier report it was observed that bilateral stereotaxic injections of 6-hydroxydopamine (6-OHDA) into the zone compacta of the substantia nigra deficits in the acquisition of a conditioned avoidance response [8], The present experiments were designed to determine if either a generalized learning impairment or a decreased sensitivity to foot shock might be the basis for the avoidance deficit. It was found that rats subjected to bilateral 6-OHDA lesions of the substantia nigra learned a light discrimination shock escape habit in as few trials as unoperated controls. This observation indicates that the integrity of the dopaminergic nigro-neostriatal system is not essential for the formation of learned associations between sensory cues and motor responses. In a second experiment it was observed that neither the shock-induced flinch nor the jump threshold was elevated after nigral lesions, suggesting that these lesions do not decrease the aversive motivational properties of foot shock. In view of these findings, the nature of the avoidance deficit produced by substantia nigra lesions is discussed with reference to the possibility that they selectively block the initiation of voluntary motor responses. According to this hypothesis, the failure of these lesions to disrupt escape responding may be due to the fact that the unconditioned stimulus generates reflexive motor responses (flinch, jump, etc.) which are sufficient to begin the motor sequences that cannot be initiated voluntarily in response to the conditioned stimulus.", "contents": "Discriminated escape learning and response to electric shock after 6-hydroxydopamine lesions of the nigro-neostriatal dopaminergic projection. In an earlier report it was observed that bilateral stereotaxic injections of 6-hydroxydopamine (6-OHDA) into the zone compacta of the substantia nigra deficits in the acquisition of a conditioned avoidance response [8], The present experiments were designed to determine if either a generalized learning impairment or a decreased sensitivity to foot shock might be the basis for the avoidance deficit. It was found that rats subjected to bilateral 6-OHDA lesions of the substantia nigra learned a light discrimination shock escape habit in as few trials as unoperated controls. This observation indicates that the integrity of the dopaminergic nigro-neostriatal system is not essential for the formation of learned associations between sensory cues and motor responses. In a second experiment it was observed that neither the shock-induced flinch nor the jump threshold was elevated after nigral lesions, suggesting that these lesions do not decrease the aversive motivational properties of foot shock. In view of these findings, the nature of the avoidance deficit produced by substantia nigra lesions is discussed with reference to the possibility that they selectively block the initiation of voluntary motor responses. According to this hypothesis, the failure of these lesions to disrupt escape responding may be due to the fact that the unconditioned stimulus generates reflexive motor responses (flinch, jump, etc.) which are sufficient to begin the motor sequences that cannot be initiated voluntarily in response to the conditioned stimulus."}
{"id": "PMID:1096177", "title": "Effects of 3-acetylpyridine on spontaneous alternation in the mouse.", "content": "When administered to mice, 3-acetylpyridine has been shown to selectively destroy the hippocampa neural fields CA3 and CA4. Adult mice, injected i.p. with150 mg/kg 3-acetylpyridine showed a reduced frequency of spontaneous alteration(48%) in a T-maze, compared with saline injcetd controls (73%). The pattern of latency change in the experimental mice was  consistent with a failure to habitatue normally. these behavioral effects of 3-acetylpyrdine resemble those observed following lesions of the hippocampus induced by stereotaxic surgery.", "contents": "Effects of 3-acetylpyridine on spontaneous alternation in the mouse. When administered to mice, 3-acetylpyridine has been shown to selectively destroy the hippocampa neural fields CA3 and CA4. Adult mice, injected i.p. with150 mg/kg 3-acetylpyridine showed a reduced frequency of spontaneous alteration(48%) in a T-maze, compared with saline injcetd controls (73%). The pattern of latency change in the experimental mice was  consistent with a failure to habitatue normally. these behavioral effects of 3-acetylpyrdine resemble those observed following lesions of the hippocampus induced by stereotaxic surgery."}
{"id": "PMID:1096182", "title": "Construction of the perineal body in the female.", "content": "A simple, one-stage operation is presented for the construction of a perineal body  in a female who has a common rectovaginal opening, or cloaca. The operation has been performed on 3 teenaged girls, with an excellent result in each. In only one instance has been necessary to perform a secondary operation for correction of the rectal incontinence.", "contents": "Construction of the perineal body in the female. A simple, one-stage operation is presented for the construction of a perineal body  in a female who has a common rectovaginal opening, or cloaca. The operation has been performed on 3 teenaged girls, with an excellent result in each. In only one instance has been necessary to perform a secondary operation for correction of the rectal incontinence."}
{"id": "PMID:1096183", "title": "The free vascularized bone graft. A clinical extension of microvascular techniques.", "content": "For the common problem of lower limb injury with extensive. Loss of skin and bone, a new method of free vascularized bone grafting has been used and integrated with an appropriate soft tissue flap repair. The technique was developed to salvage two legs which would otherwise have been amputated. The preliminary result in Case 2 is encouraging. This case would appear to be the first successful distant transfer of a composite fibular graft by microvascular anastomoses to be reported in man.", "contents": "The free vascularized bone graft. A clinical extension of microvascular techniques. For the common problem of lower limb injury with extensive. Loss of skin and bone, a new method of free vascularized bone grafting has been used and integrated with an appropriate soft tissue flap repair. The technique was developed to salvage two legs which would otherwise have been amputated. The preliminary result in Case 2 is encouraging. This case would appear to be the first successful distant transfer of a composite fibular graft by microvascular anastomoses to be reported in man."}
{"id": "PMID:1096184", "title": "Surgical treatment of hidradenitis suppurativa of the axilla.", "content": "We review our surgical experience with hidradenitis suppurativa of the axilla at Cook County Hospital during the years 1963 to 1972. The operation for this entity described by Pollock et al has (1) reduced the length of hospitalization, (2) increased the rate of primary healing and reduced the period of secondary healing, (3) allowed a great amount of tissue to be excised, (4) permitted both axillae to be operated on at once (without incapacitating the patient), (5) avoided the need for skin grafting, and (6) has had less morbidity for those patients in whom primary healing is not achieved. We believe the method reported by Pollock et al is now the treatment of choice for this disease.", "contents": "Surgical treatment of hidradenitis suppurativa of the axilla. We review our surgical experience with hidradenitis suppurativa of the axilla at Cook County Hospital during the years 1963 to 1972. The operation for this entity described by Pollock et al has (1) reduced the length of hospitalization, (2) increased the rate of primary healing and reduced the period of secondary healing, (3) allowed a great amount of tissue to be excised, (4) permitted both axillae to be operated on at once (without incapacitating the patient), (5) avoided the need for skin grafting, and (6) has had less morbidity for those patients in whom primary healing is not achieved. We believe the method reported by Pollock et al is now the treatment of choice for this disease."}
{"id": "PMID:1096185", "title": "Reconstruction of mandibular defects after radiation, using a free, living bone graft transferred by microvascular anastomose. An experimental study.", "content": "In a previous publication we described the replacement of a mandibular defect by a free, composite rib graft, transferred by microvascular anastomoses of the posterior intercostal vessels to donor vessels in the neck. We now present data which demonstrate that successful results can be achieved even after radical mandibular radiation. This technique, done in dogs, has obvious implications in the management of oral cancer in man.", "contents": "Reconstruction of mandibular defects after radiation, using a free, living bone graft transferred by microvascular anastomose. An experimental study. In a previous publication we described the replacement of a mandibular defect by a free, composite rib graft, transferred by microvascular anastomoses of the posterior intercostal vessels to donor vessels in the neck. We now present data which demonstrate that successful results can be achieved even after radical mandibular radiation. This technique, done in dogs, has obvious implications in the management of oral cancer in man."}
{"id": "PMID:1096186", "title": "Free groin flaps in children.", "content": "We report two successful transfers of free groin flaps to the lower legs of children. We believe this method can be safely applied and can often take the place of distant pedicled flap transfers in children.", "contents": "Free groin flaps in children. We report two successful transfers of free groin flaps to the lower legs of children. We believe this method can be safely applied and can often take the place of distant pedicled flap transfers in children."}
{"id": "PMID:1096188", "title": "Panniculectomy specimens as a convenient and inexpensive of homograft skin.", "content": "Panniculectomy specimens provide a convenient and inexpensive source of skin homografts when harvested in the operating room under sterile conditions.", "contents": "Panniculectomy specimens as a convenient and inexpensive of homograft skin. Panniculectomy specimens provide a convenient and inexpensive source of skin homografts when harvested in the operating room under sterile conditions."}
{"id": "PMID:1096190", "title": "Neurovascular free flaps. A preliminary report.", "content": "An experimental, anatomical, and clinical study has confirmed the feasibility of free neurovascular flaps.  The interrelationship between the cutaneous vascular and neural supply has been defined and demonstrated electrophysiologically, both in man and in experimental animals.  A potential donor site in man has been explored.  Free neurovascular flap transfers by microneurovascular anastomoses are technically feasible.  Clinical success will occur with the identification of suitable donor sites.", "contents": "Neurovascular free flaps. A preliminary report. An experimental, anatomical, and clinical study has confirmed the feasibility of free neurovascular flaps.  The interrelationship between the cutaneous vascular and neural supply has been defined and demonstrated electrophysiologically, both in man and in experimental animals.  A potential donor site in man has been explored.  Free neurovascular flap transfers by microneurovascular anastomoses are technically feasible.  Clinical success will occur with the identification of suitable donor sites."}
{"id": "PMID:1096191", "title": "Burned hands.", "content": "Hand involvement is common in patients with severe burns.  Our experience indicates that almost one-third of the patients admitted to the Shriner's Burns Institute and The University of Texas Medical Branch Hospitals with burns exceeding 30 per cent of the body surface had concomitant burns of the hand.  Our approach in managing the burned hand during the acute phase of unjury, as well as during secondary reconstruction of the deformities, is presented and discussed.", "contents": "Burned hands. Hand involvement is common in patients with severe burns.  Our experience indicates that almost one-third of the patients admitted to the Shriner's Burns Institute and The University of Texas Medical Branch Hospitals with burns exceeding 30 per cent of the body surface had concomitant burns of the hand.  Our approach in managing the burned hand during the acute phase of unjury, as well as during secondary reconstruction of the deformities, is presented and discussed."}
{"id": "PMID:1096192", "title": "Maxillary arch alignment in the bilateral cleft lip and palate infant, using pinned coaxial screw appliance.", "content": "An arch alignment appliance, capable of both maxillary expansion and premaxillary retraction, based on a pinned screw mechanism and capable of extraoral activation, is described and illustrated.", "contents": "Maxillary arch alignment in the bilateral cleft lip and palate infant, using pinned coaxial screw appliance. An arch alignment appliance, capable of both maxillary expansion and premaxillary retraction, based on a pinned screw mechanism and capable of extraoral activation, is described and illustrated."}
{"id": "PMID:1096193", "title": "Secondary reconstruction of the cervical esophagus.", "content": "Ten patients have had secondary reconstructions of the cervical esophagus after ablation of the laryngopharyngeal complex for cancer. Our use of deltopectoral flaps for this is described.", "contents": "Secondary reconstruction of the cervical esophagus. Ten patients have had secondary reconstructions of the cervical esophagus after ablation of the laryngopharyngeal complex for cancer. Our use of deltopectoral flaps for this is described."}
{"id": "PMID:1096196", "title": "Malar implants for improvement of the facial appearance.", "content": "Esthetic correction of flat malar eminences may contribute to the harmony of facial proportions by making the face more oval and by giving a more youthful appearance. Zygomatic augmentation may also be indicated for the treatment of congenital hypoplasias, asymmetries, or traumatic depressions of the malar region. We have used the procedure in 52 cases since 1964. For augmentation we use a perforated soft, silicone rubber implant. The technique and results are described. The results have been satisfactory, for both the patients and the surgeon.", "contents": "Malar implants for improvement of the facial appearance. Esthetic correction of flat malar eminences may contribute to the harmony of facial proportions by making the face more oval and by giving a more youthful appearance. Zygomatic augmentation may also be indicated for the treatment of congenital hypoplasias, asymmetries, or traumatic depressions of the malar region. We have used the procedure in 52 cases since 1964. For augmentation we use a perforated soft, silicone rubber implant. The technique and results are described. The results have been satisfactory, for both the patients and the surgeon."}
{"id": "PMID:1096197", "title": "The nerve gap: suture under tension vs. graft.", "content": "Conduction velocities and amplitudes of evoked responses were used in experimental models to compare reinnervation through nerve gaps sutured under tension or bridged with nerve grafts. The best results were obtained when end-to-end suture was done without tension. Regeneration through mildly stretched nerve repairs was equivalent to applying a properly tailored graft. Minimal axonal activity was exhibited by severely stretched repair sites.", "contents": "The nerve gap: suture under tension vs. graft. Conduction velocities and amplitudes of evoked responses were used in experimental models to compare reinnervation through nerve gaps sutured under tension or bridged with nerve grafts. The best results were obtained when end-to-end suture was done without tension. Regeneration through mildly stretched nerve repairs was equivalent to applying a properly tailored graft. Minimal axonal activity was exhibited by severely stretched repair sites."}
{"id": "PMID:1096201", "title": "Stoicism and paranoia.", "content": "A paranoid strain is manifest in Stoic utterances generally, especially in the Stoic conception of autarky, where the Sage regards himself as distinctly \"other\" in the midst of society, and indifferent to its values, except as he dissembles his indifference. Now, influential theories of paranoia stress a projective process: for Freudians, the projection of repressed homosexual libido in altered form, for Ovesey, a \"pseudohomosexual\" anxiety arising out of a maladaptation in the male to the culturally assigned competitive male role, a failure perceived as effeminate. But Stoicism took root in the culture (Classical Greece) where homosexuality, far from being universally regarded as degenerate, was widely extolled as the norm for superior men and the most advantageous frame work for the tutelage of aristocratic boys. It would seem unlikely that paranoia in this context would reflect uneasiness over unconscious homoerotic (or distorted pseudo-erotic) strivings. What light is shed on the purported projection of either homosexual or pseudo-homosexual anxiety by paranoid elements in Stoicism?", "contents": "Stoicism and paranoia. A paranoid strain is manifest in Stoic utterances generally, especially in the Stoic conception of autarky, where the Sage regards himself as distinctly \"other\" in the midst of society, and indifferent to its values, except as he dissembles his indifference. Now, influential theories of paranoia stress a projective process: for Freudians, the projection of repressed homosexual libido in altered form, for Ovesey, a \"pseudohomosexual\" anxiety arising out of a maladaptation in the male to the culturally assigned competitive male role, a failure perceived as effeminate. But Stoicism took root in the culture (Classical Greece) where homosexuality, far from being universally regarded as degenerate, was widely extolled as the norm for superior men and the most advantageous frame work for the tutelage of aristocratic boys. It would seem unlikely that paranoia in this context would reflect uneasiness over unconscious homoerotic (or distorted pseudo-erotic) strivings. What light is shed on the purported projection of either homosexual or pseudo-homosexual anxiety by paranoid elements in Stoicism?"}
{"id": "PMID:1096216", "title": "Apomorphine-induced rotation in normal rats and interaction with unilateral caudate lesions.", "content": "Apomorphine (i.p.) induced rotational behavior (i.e. circling) in normal unoperated rats. This rotation increased with increasing dose up to 10.0 mg/kg, after which the dose-response curve appeared to plateau. Although there was large variability among rats, rotation for each rat was consistent in both direction and magnitude from week to week. Rotation was not antagonized by alpha-methyl-para-tyrosine. When rats with unilateral lesions of the caudate nucleus were tested with apomorphine, postoperative rotation was significantly influenced by the direction of preoperative rotation; rats rotated more postoperatively if the lesion was made ipsilateral rather than contralateral to their preoperative direction of rotation. These results suggest that there is a bilateral asymmetry of dopaminergic receptors in the nigro-striatal pathways of normal rats.", "contents": "Apomorphine-induced rotation in normal rats and interaction with unilateral caudate lesions. Apomorphine (i.p.) induced rotational behavior (i.e. circling) in normal unoperated rats. This rotation increased with increasing dose up to 10.0 mg/kg, after which the dose-response curve appeared to plateau. Although there was large variability among rats, rotation for each rat was consistent in both direction and magnitude from week to week. Rotation was not antagonized by alpha-methyl-para-tyrosine. When rats with unilateral lesions of the caudate nucleus were tested with apomorphine, postoperative rotation was significantly influenced by the direction of preoperative rotation; rats rotated more postoperatively if the lesion was made ipsilateral rather than contralateral to their preoperative direction of rotation. These results suggest that there is a bilateral asymmetry of dopaminergic receptors in the nigro-striatal pathways of normal rats."}
{"id": "PMID:1096217", "title": "The effect of cyproheptadine on hunger, calorie intake and body weight in man.", "content": "The appetite stimulating action and the weight gaining potential of one month's treatment with the antihistamine and antiserotonergic compound cyproheptadine (Periactin) 4 mg three times daily was compared to placebo in a double-blind crossover trial in sixteen thin but otherwise normal volunteers who wanted to gain weight. Subjects gained significantly more weight on cyproheptadine than on placebo. There was also a corresponding relative increase in subjective hunger ratings and food intake during the period on active drug. Drowsiness was the most frequent side effect observed. These findings are discussed in relation to a possible serotonergic feeding mechanism.", "contents": "The effect of cyproheptadine on hunger, calorie intake and body weight in man. The appetite stimulating action and the weight gaining potential of one month's treatment with the antihistamine and antiserotonergic compound cyproheptadine (Periactin) 4 mg three times daily was compared to placebo in a double-blind crossover trial in sixteen thin but otherwise normal volunteers who wanted to gain weight. Subjects gained significantly more weight on cyproheptadine than on placebo. There was also a corresponding relative increase in subjective hunger ratings and food intake during the period on active drug. Drowsiness was the most frequent side effect observed. These findings are discussed in relation to a possible serotonergic feeding mechanism."}
{"id": "PMID:1096218", "title": "Extrapyramidal reactions and amine metabolites in cerebrospinal fluid during haloperidol and clozapine treatment of schizophrenic patients.", "content": "8 male schizophrenic patients participated in a double-blind, cross over study of the extrapyramidal side-effects of haloperidol and clozapine (acute dystonis, Parkinsonism and tardive dyskinesia), together with their effect on homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the cerebrospinal fluid (CSF). Haloperidol (9 mg/day) caused Parkinsonism, reduced tardive dyskinesias and increased the HVA concentration in the CSF. Clozapine (225 mg/day) had no effect on the neurological phenomena but reduced HVA and 5-HIAA concentrations in the CSF. During the discontinuation phase following the administration of haloperidol, tardive dyskinesia occurred or was aggravated; this did not occur after administration of clozapine. Accordingly, it is suggested that clozapine does not induce dopaminergic hypersensibility and, therefore, will not induce tardive dyskinesias.", "contents": "Extrapyramidal reactions and amine metabolites in cerebrospinal fluid during haloperidol and clozapine treatment of schizophrenic patients. 8 male schizophrenic patients participated in a double-blind, cross over study of the extrapyramidal side-effects of haloperidol and clozapine (acute dystonis, Parkinsonism and tardive dyskinesia), together with their effect on homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the cerebrospinal fluid (CSF). Haloperidol (9 mg/day) caused Parkinsonism, reduced tardive dyskinesias and increased the HVA concentration in the CSF. Clozapine (225 mg/day) had no effect on the neurological phenomena but reduced HVA and 5-HIAA concentrations in the CSF. During the discontinuation phase following the administration of haloperidol, tardive dyskinesia occurred or was aggravated; this did not occur after administration of clozapine. Accordingly, it is suggested that clozapine does not induce dopaminergic hypersensibility and, therefore, will not induce tardive dyskinesias."}
{"id": "PMID:1096219", "title": "[Effects of (--)delta-9-trans-tetrahydrocannabinol (delta-9-THC) on tests of psychoticism. A study of Eysenck's drug postulate on the effects of hallucinogens in relation to psychoticism (author's transl)].", "content": "In a double-blind study on 36 volunteers the effects of 250 mug/kg (--)delta-9-trans-tetrahydrocannabinol p.o. on objective and performance tests measuring psychoticism according to Eysenck were assessed. The hypothesis was tested that subjects treated with delta-9-THC differ from those receiving placebo in the same way as endogenous psychotics differ from normals. Confirmation was obtained in only 5 out of 19 variables. Four of these 5 variables may be considered as tests of concentration.", "contents": "[Effects of (--)delta-9-trans-tetrahydrocannabinol (delta-9-THC) on tests of psychoticism. A study of Eysenck's drug postulate on the effects of hallucinogens in relation to psychoticism (author's transl)]. In a double-blind study on 36 volunteers the effects of 250 mug/kg (--)delta-9-trans-tetrahydrocannabinol p.o. on objective and performance tests measuring psychoticism according to Eysenck were assessed. The hypothesis was tested that subjects treated with delta-9-THC differ from those receiving placebo in the same way as endogenous psychotics differ from normals. Confirmation was obtained in only 5 out of 19 variables. Four of these 5 variables may be considered as tests of concentration."}
{"id": "PMID:1096224", "title": "The repair of potentially lethal damage; an alternative approach.", "content": "An alternative approach to the explanation and analysis of post-irradiation effects is presented. The analysis is based on the repair of a radiation-induced lesion and it is shown that the experimental results are compatible with the repair of one type of critical lesion involved in cell reproductive death. The nature of the lesion with respect to potentially lethal damage and sub-lethal damage is discussed.", "contents": "The repair of potentially lethal damage; an alternative approach. An alternative approach to the explanation and analysis of post-irradiation effects is presented. The analysis is based on the repair of a radiation-induced lesion and it is shown that the experimental results are compatible with the repair of one type of critical lesion involved in cell reproductive death. The nature of the lesion with respect to potentially lethal damage and sub-lethal damage is discussed."}
{"id": "PMID:1096233", "title": "Osmotic Nephrosis Induced by Water-Soluble Triiodinated Contrast Media in Man. A Retrospective Study of 47 Cases.", "content": "Renal biopsies were performed in 211 patients which 10 days of excretory urography or renal arteriogrpahy in which diatrizoate, iothalamate or ioxithalamate had been used. In 47 renal specimens, osmotic nephrosis of the proximal tubular cells was found. Previous renal function had been normal in 10 patients, moderately impaired in 19, and severely impaired in 18. Tubular atrophy and/or necrosis was associated with histological features in 29 of 47 patients. Diffuse osmotic nephrosis was more often found in patients biopsied soon after roentgenography and also with severe renal insufficiency, but was not necessarily associated with declining renal function. The mechanism(s) by which contrast media may induce osmotic nephrosis remains unclear.", "contents": "Osmotic Nephrosis Induced by Water-Soluble Triiodinated Contrast Media in Man. A Retrospective Study of 47 Cases. Renal biopsies were performed in 211 patients which 10 days of excretory urography or renal arteriogrpahy in which diatrizoate, iothalamate or ioxithalamate had been used. In 47 renal specimens, osmotic nephrosis of the proximal tubular cells was found. Previous renal function had been normal in 10 patients, moderately impaired in 19, and severely impaired in 18. Tubular atrophy and/or necrosis was associated with histological features in 29 of 47 patients. Diffuse osmotic nephrosis was more often found in patients biopsied soon after roentgenography and also with severe renal insufficiency, but was not necessarily associated with declining renal function. The mechanism(s) by which contrast media may induce osmotic nephrosis remains unclear."}
{"id": "PMID:1096234", "title": "Computer Diagnosis of Breast Thermograms.", "content": "A computer-based technique was developed for the automated diagnosis of breast thermogram. Eighty-five thermograms were digitized and analyzed, and 23 parameters computed for each thermogram. A statistical decision program, based on a linear discriminant analysis technique, classified the thermograms into normal and abnormal categories. The accuracy of classification was evaluated by several techniques and found to be comparable to that of trained thermographers.", "contents": "Computer Diagnosis of Breast Thermograms. A computer-based technique was developed for the automated diagnosis of breast thermogram. Eighty-five thermograms were digitized and analyzed, and 23 parameters computed for each thermogram. A statistical decision program, based on a linear discriminant analysis technique, classified the thermograms into normal and abnormal categories. The accuracy of classification was evaluated by several techniques and found to be comparable to that of trained thermographers."}
{"id": "PMID:1096235", "title": "The Use of Computer Tomography in Ophthalmology.", "content": "Fifty-three orbital lesions of various types were studied by computer tomography to assess the capabilities and limitations of this technique. Although CT scanning in ophthalmology is in its infancy, results showed that it is useful in the investigation of a number of pathological conditions, including congenital anomalies, arteriovenous malformations, bone lesions, trauma, inflammation, granulomas, pseudotumors, epidermoid cysts, and tumors arising in or near the optic nerve, behind the optic bulb, or in the lacrimal region, as well as changes following eye surgery.", "contents": "The Use of Computer Tomography in Ophthalmology. Fifty-three orbital lesions of various types were studied by computer tomography to assess the capabilities and limitations of this technique. Although CT scanning in ophthalmology is in its infancy, results showed that it is useful in the investigation of a number of pathological conditions, including congenital anomalies, arteriovenous malformations, bone lesions, trauma, inflammation, granulomas, pseudotumors, epidermoid cysts, and tumors arising in or near the optic nerve, behind the optic bulb, or in the lacrimal region, as well as changes following eye surgery."}
{"id": "PMID:1096254", "title": "Cultures of pig macrophages.", "content": "Freshly collected blood from pigs was defibrinated with glass beads. The white cells were collected on a Ficoll-Hypaque mixture and grown in RPMI 1640 medium containing 20 per cent fetal calf serum. Monocytes adhered to the plastic or glass flasks and the erythrocytes and lymphocytes remained in suspension. The adherent cells assumed the appearance of macrophages and the mean cell size at eight days was 40 mum. Phagocytosis was demonstrated and, in the first few days, incorporation of tritiated thymidine occurred. Cells remained viable in culture for more than eight weeks. Histo-chemical studies and the appearance of the cells under the electron microscope are described.", "contents": "Cultures of pig macrophages. Freshly collected blood from pigs was defibrinated with glass beads. The white cells were collected on a Ficoll-Hypaque mixture and grown in RPMI 1640 medium containing 20 per cent fetal calf serum. Monocytes adhered to the plastic or glass flasks and the erythrocytes and lymphocytes remained in suspension. The adherent cells assumed the appearance of macrophages and the mean cell size at eight days was 40 mum. Phagocytosis was demonstrated and, in the first few days, incorporation of tritiated thymidine occurred. Cells remained viable in culture for more than eight weeks. Histo-chemical studies and the appearance of the cells under the electron microscope are described."}
{"id": "PMID:1096275", "title": "A double-blind cross-over trail of Prenazone and aspirin in the management of rheumatoid arthritis.", "content": "A double-blind cross-over trial of Prenazone 600 mg and Aspirin 4 g daily was carried out on 20 patients suffering with rheumatoid arthritis. The analgesic and anti-inflammmatory activity was indistinguishable from that of aspirin under the conditions of the trail. Twelve patients expressed a general preference for Prenazone and six for aspirin. Prenazone appeared to be well tolerated and free from serious side-effects. These results suggest that it will be a useful drug in the management of rheumatoid arthritis.", "contents": "A double-blind cross-over trail of Prenazone and aspirin in the management of rheumatoid arthritis. A double-blind cross-over trial of Prenazone 600 mg and Aspirin 4 g daily was carried out on 20 patients suffering with rheumatoid arthritis. The analgesic and anti-inflammmatory activity was indistinguishable from that of aspirin under the conditions of the trail. Twelve patients expressed a general preference for Prenazone and six for aspirin. Prenazone appeared to be well tolerated and free from serious side-effects. These results suggest that it will be a useful drug in the management of rheumatoid arthritis."}
{"id": "PMID:1096276", "title": "A double-blind cross-over trial of Prenazone (DA 2370) and phenylbutazone in rheumatoid arthritis.", "content": "A short-term double-bind cross-over trial of prenazone 600 mg daily and phenylbutazone 300 mg daily was carried out in twenty out-patients with rheumatoid arthritis. Ten patients preferred prenazone, and eight preferred phenylbutazone. No significant difference in analgesic and antiinflammatory potency was demonstrated under the conditions of the trial, and no serious adverse effects, either clinical or laboratory, were encountered. These findings support other claims that prenazone may be a useful alternative in the treatment of rheumatoid arthritis.", "contents": "A double-blind cross-over trial of Prenazone (DA 2370) and phenylbutazone in rheumatoid arthritis. A short-term double-bind cross-over trial of prenazone 600 mg daily and phenylbutazone 300 mg daily was carried out in twenty out-patients with rheumatoid arthritis. Ten patients preferred prenazone, and eight preferred phenylbutazone. No significant difference in analgesic and antiinflammatory potency was demonstrated under the conditions of the trial, and no serious adverse effects, either clinical or laboratory, were encountered. These findings support other claims that prenazone may be a useful alternative in the treatment of rheumatoid arthritis."}
{"id": "PMID:1096285", "title": "[Rheumatoid synovial fluid].", "content": "In making a comparative study of different parameters in the examination of synovial fluid (rheumatoid factors, ragocytes, leucocytes, synovial complement, immunoglobulins) the authors show that rheumatoid fluid has characteristics that make differentiation from other inflammatory arthropathies possible on a statistical basis, thanks to the test for the homogeneity of the means. The presence of these characteristics seems to constitute a diagnostic argument which can be measured by mathematical methods.", "contents": "[Rheumatoid synovial fluid]. In making a comparative study of different parameters in the examination of synovial fluid (rheumatoid factors, ragocytes, leucocytes, synovial complement, immunoglobulins) the authors show that rheumatoid fluid has characteristics that make differentiation from other inflammatory arthropathies possible on a statistical basis, thanks to the test for the homogeneity of the means. The presence of these characteristics seems to constitute a diagnostic argument which can be measured by mathematical methods."}
{"id": "PMID:1096286", "title": "[Quantitative histological study of the cartilage of human arthrotic femur head].", "content": "A quantitative histological study of the cartilage of the human arthrosic femoral head. In this work, samples of unsplit cartilage taken from 15 arthrosic femoral heads and 26 normal femoral heads from the same topographical areas, and of identical age, were studied by quantitative histological methods. This study was concerned with the thickness of the calcified and non-calcified cartilage, the cellular density, the average number of nuclei per lacuna, and the average surface area of the lacunae in the different layers of the cartilage. Comparison between the two groups of samples showed that the arthrosic cartilage differed from the normal cartilage by a diminution in the size of the superficial lacunae, a reduction in the number of nuclei per lacuna in the middle layer, a diminution in the cellularity of the deep layer and an augmentation in the layer of calcified cartilage. These modifications suggest that arthrosis of the hip is secondary to diffuse cellular alterations associating a diminution in metabolic activity and necrosis of the chondrocytes.", "contents": "[Quantitative histological study of the cartilage of human arthrotic femur head]. A quantitative histological study of the cartilage of the human arthrosic femoral head. In this work, samples of unsplit cartilage taken from 15 arthrosic femoral heads and 26 normal femoral heads from the same topographical areas, and of identical age, were studied by quantitative histological methods. This study was concerned with the thickness of the calcified and non-calcified cartilage, the cellular density, the average number of nuclei per lacuna, and the average surface area of the lacunae in the different layers of the cartilage. Comparison between the two groups of samples showed that the arthrosic cartilage differed from the normal cartilage by a diminution in the size of the superficial lacunae, a reduction in the number of nuclei per lacuna in the middle layer, a diminution in the cellularity of the deep layer and an augmentation in the layer of calcified cartilage. These modifications suggest that arthrosis of the hip is secondary to diffuse cellular alterations associating a diminution in metabolic activity and necrosis of the chondrocytes."}
{"id": "PMID:1096290", "title": "Genetic control of B-cell responses. I. Selective unresponsiveness to lipopolysaccharide.", "content": "Spleen cells from C3H/HeJ mice fail to develop both proliferative responses and increased polyclonal antibody secretion in the presence of concentrations of the B-cell mitogen lipopolysaccharide that are optimal for the induction of B-cell responses in conventional strains. This unresponsiveness is selective for lipopolysaccharide, since C3H/HeJ spleen cells respond normally to two other polyclonal B-cell activators-dextran-sulphate and purified protein derivative of tuberculin. These findings are interpreted as indicating a selective defect in the B-cell subpopulation that responds to lipopolysaccharides in conventional strains.", "contents": "Genetic control of B-cell responses. I. Selective unresponsiveness to lipopolysaccharide. Spleen cells from C3H/HeJ mice fail to develop both proliferative responses and increased polyclonal antibody secretion in the presence of concentrations of the B-cell mitogen lipopolysaccharide that are optimal for the induction of B-cell responses in conventional strains. This unresponsiveness is selective for lipopolysaccharide, since C3H/HeJ spleen cells respond normally to two other polyclonal B-cell activators-dextran-sulphate and purified protein derivative of tuberculin. These findings are interpreted as indicating a selective defect in the B-cell subpopulation that responds to lipopolysaccharides in conventional strains."}
{"id": "PMID:1096291", "title": "Serum factors causing impaired macrophage function in systemic lupus erythematosus.", "content": "Leukocytes from patients with systemic lupus erythematosus (SLE) and healthy controls were cultured for 4 days in either SLE serum or control serum. The remaining monolayer of monocyte-derived macrophages was allowed to ingest yeast cells. Macrophages (from SLE patients or controls) incubated in SLE sera presented impaired phagocytic activity and glass adherence compared with cells grown in control sera. SLE sera contain one or more factors that impair macrophage function; IgG-containing immune complexes may be one such factor.", "contents": "Serum factors causing impaired macrophage function in systemic lupus erythematosus. Leukocytes from patients with systemic lupus erythematosus (SLE) and healthy controls were cultured for 4 days in either SLE serum or control serum. The remaining monolayer of monocyte-derived macrophages was allowed to ingest yeast cells. Macrophages (from SLE patients or controls) incubated in SLE sera presented impaired phagocytic activity and glass adherence compared with cells grown in control sera. SLE sera contain one or more factors that impair macrophage function; IgG-containing immune complexes may be one such factor."}
{"id": "PMID:1096292", "title": "Human interferon therapy for herpes zoster in adults.", "content": "Of 37 patients with herpes zoster 28 were treated with human exogenous interferon and 9 received only culture medium. The interferon was produced in leukocyte cultures and was given intramuscularly in one daily dose of 1 million units for 5-8 days. In the interferon-treated patients interferon was detectable in serum (peak level 1-5 hours after interferon injections) and in vesicle fluid, and in some patients also in urine samples. Anti-interferon antibodies were not found. Of the 28 interferon-treated patients 8 had a slight temperature increase and 4 showed a transient local reaction. In the interferon treated group of patients the duration of pain was shortened and the development of crust formation was enhanced compared with the control group.", "contents": "Human interferon therapy for herpes zoster in adults. Of 37 patients with herpes zoster 28 were treated with human exogenous interferon and 9 received only culture medium. The interferon was produced in leukocyte cultures and was given intramuscularly in one daily dose of 1 million units for 5-8 days. In the interferon-treated patients interferon was detectable in serum (peak level 1-5 hours after interferon injections) and in vesicle fluid, and in some patients also in urine samples. Anti-interferon antibodies were not found. Of the 28 interferon-treated patients 8 had a slight temperature increase and 4 showed a transient local reaction. In the interferon treated group of patients the duration of pain was shortened and the development of crust formation was enhanced compared with the control group."}
{"id": "PMID:1096293", "title": "Epidemiological aspects of impetigo contagiosa in western Sweden.", "content": "200 patients with primary impetigo were studied. Staphylococcus aureus was the only organism isolated in 72.5%, beta-hemolytic streptococci, group A, in 10%, and both pathogens in 15%. 17 of the 50 streptococcal isolates were typed serologically; 11 of these belonged to potentially nephritogenic types. A majority of these cases had identical strains in the nose and throat. Hence, the primary source of skin streptococcal in this study may be the nasopharynx. Uncomplicated cases were treated with topical antibiotics, and oral antibiotics were added in 22 cases with extensive lesions, fever or a concurrent throat infection. None of the 200 patients developed acute glomerulonephritis and urinary analyses were normal at follow-up after 3 weeks.", "contents": "Epidemiological aspects of impetigo contagiosa in western Sweden. 200 patients with primary impetigo were studied. Staphylococcus aureus was the only organism isolated in 72.5%, beta-hemolytic streptococci, group A, in 10%, and both pathogens in 15%. 17 of the 50 streptococcal isolates were typed serologically; 11 of these belonged to potentially nephritogenic types. A majority of these cases had identical strains in the nose and throat. Hence, the primary source of skin streptococcal in this study may be the nasopharynx. Uncomplicated cases were treated with topical antibiotics, and oral antibiotics were added in 22 cases with extensive lesions, fever or a concurrent throat infection. None of the 200 patients developed acute glomerulonephritis and urinary analyses were normal at follow-up after 3 weeks."}
{"id": "PMID:1096294", "title": "Effect of circulating norepinephrine on the renin release from the denervated kidney.", "content": "The effect of renal denervation on the reactivity of the renin release mechanism to circulating norepinephrine was studied in six dogs with renal autografts. The renin release was determined simultaneously in the transplanted and the untouched kidney. Both kidneys showed an increased release of renin when norepinephrine was given. A slight reduction in renal blood flow and increase in arterial blood pressure was recorded. Propranolol blocked the release of renin induced by norepinephrine without affecting either the reduced blood flow thorugh the autograft or the elevated blood pressure. The denervation of the autotransplanted kidney was histochemically verified. The findings indicate that the renin release induced by circulating norepinephrine is independent of the intrarenal adrenergic nerves, and rather suggest that it is the result of an action of norepinephrine directly on the renin-producing juxtaglomerular cells. In this mechanism a beta-adrenergic receptor system appears to be involved.", "contents": "Effect of circulating norepinephrine on the renin release from the denervated kidney. The effect of renal denervation on the reactivity of the renin release mechanism to circulating norepinephrine was studied in six dogs with renal autografts. The renin release was determined simultaneously in the transplanted and the untouched kidney. Both kidneys showed an increased release of renin when norepinephrine was given. A slight reduction in renal blood flow and increase in arterial blood pressure was recorded. Propranolol blocked the release of renin induced by norepinephrine without affecting either the reduced blood flow thorugh the autograft or the elevated blood pressure. The denervation of the autotransplanted kidney was histochemically verified. The findings indicate that the renin release induced by circulating norepinephrine is independent of the intrarenal adrenergic nerves, and rather suggest that it is the result of an action of norepinephrine directly on the renin-producing juxtaglomerular cells. In this mechanism a beta-adrenergic receptor system appears to be involved."}
{"id": "PMID:1096295", "title": "Condylomata acuminata of the bladder.", "content": "A case of invasion of the bladder by condylomata acuminata in a kidney-transplanted patient under immunosuppressive treatment is presented. Conservative treatment with application of podophyllin and transurethral resection was carried out. There were numerous recurrences, but so far it has been possible to control the disease, in contrast to the four cases found in the literature, which have all resulted in total cystectomy.", "contents": "Condylomata acuminata of the bladder. A case of invasion of the bladder by condylomata acuminata in a kidney-transplanted patient under immunosuppressive treatment is presented. Conservative treatment with application of podophyllin and transurethral resection was carried out. There were numerous recurrences, but so far it has been possible to control the disease, in contrast to the four cases found in the literature, which have all resulted in total cystectomy."}
{"id": "PMID:1096296", "title": "Immunological aspects of sarcoidosis.", "content": "Circulating immune complexes were found in sera from patients with acute sarcoidosis during the initial phase of the disease. The symptoms of acute sarcoidosis may be explained by the presence of such complexes. The total number of peripheral T cells was lower in most patients with sarcoidosis than in healthy controls. The difference was most marked in cases with long duration of the disease. The composition of peripheral T cells was altered irrespective of the total number of such cells, and a lack of cells responding to PPD and con A was shown. Tis may be related to the impairment of delayed hypersensitivity reactions which may be present in all stages of the disease. The atypical mononuclear cells which have been found in blood from patients with sarcoidosis are lymphocytic cells. Some have B cell properties whereas some seem to have T cell origin. The presence of such cells may indicate a persistent activation of the immune system. Immunological reactivity determined by genes closely linked to the major histocompatibility complex does not seem to be responsible for susceptibility to sarcoidosis.", "contents": "Immunological aspects of sarcoidosis. Circulating immune complexes were found in sera from patients with acute sarcoidosis during the initial phase of the disease. The symptoms of acute sarcoidosis may be explained by the presence of such complexes. The total number of peripheral T cells was lower in most patients with sarcoidosis than in healthy controls. The difference was most marked in cases with long duration of the disease. The composition of peripheral T cells was altered irrespective of the total number of such cells, and a lack of cells responding to PPD and con A was shown. Tis may be related to the impairment of delayed hypersensitivity reactions which may be present in all stages of the disease. The atypical mononuclear cells which have been found in blood from patients with sarcoidosis are lymphocytic cells. Some have B cell properties whereas some seem to have T cell origin. The presence of such cells may indicate a persistent activation of the immune system. Immunological reactivity determined by genes closely linked to the major histocompatibility complex does not seem to be responsible for susceptibility to sarcoidosis."}
{"id": "PMID:1096297", "title": "Live attenuated influenza virus vaccines in patients with chronic broncho-pulmonary diseases. Clinical and immunological evaluation.", "content": "The safety and potency of two live attenuated influenza A virus vaccines, administered intranasally, were tested in outpatients suffering from chronic obstructive lung disease, during two successive trials performed between 1972 and 1974. The vaccine strains were representative of the prevalent influenza A virus types; the Ann strain was derived from a classical H3N2 (A/Hong-Kong/878/69) isolated and the Alice strain from a recent H3N2 drift (A/England/42/72). The serum and nasal antibody responses were studied in a total of 40 vaccinees. When a sufficient virus dose was administered, the hemagglutination-inhibition (HI) seroconversion rate was, respectively, 86 and 73% for each vaccine trial in patients with low (less than or equal to 32) prevaccination antibody titers. A booster effect was also observed in some subjects with higher prevaccination titers. In the two trials, clear-cut rises in local antibody activity, as tested in nasal washings' samples were found in, respectively, 92 and 75% of the patients devoid of initial titer before vaccination and in 40 and 37%, respectively, of the subjects having low prevaccination titers. Clinical symptoms were observed in 29% of the cases; they were, however, mild and transient and their occurrence was not necessarily related to the vaccination. Two administrations of intranasal attenuated virus appear therefore to be safe and to lead to a satisfactory antibody response in this \"high risk\" group of patients.", "contents": "Live attenuated influenza virus vaccines in patients with chronic broncho-pulmonary diseases. Clinical and immunological evaluation. The safety and potency of two live attenuated influenza A virus vaccines, administered intranasally, were tested in outpatients suffering from chronic obstructive lung disease, during two successive trials performed between 1972 and 1974. The vaccine strains were representative of the prevalent influenza A virus types; the Ann strain was derived from a classical H3N2 (A/Hong-Kong/878/69) isolated and the Alice strain from a recent H3N2 drift (A/England/42/72). The serum and nasal antibody responses were studied in a total of 40 vaccinees. When a sufficient virus dose was administered, the hemagglutination-inhibition (HI) seroconversion rate was, respectively, 86 and 73% for each vaccine trial in patients with low (less than or equal to 32) prevaccination antibody titers. A booster effect was also observed in some subjects with higher prevaccination titers. In the two trials, clear-cut rises in local antibody activity, as tested in nasal washings' samples were found in, respectively, 92 and 75% of the patients devoid of initial titer before vaccination and in 40 and 37%, respectively, of the subjects having low prevaccination titers. Clinical symptoms were observed in 29% of the cases; they were, however, mild and transient and their occurrence was not necessarily related to the vaccination. Two administrations of intranasal attenuated virus appear therefore to be safe and to lead to a satisfactory antibody response in this \"high risk\" group of patients."}
{"id": "PMID:1096300", "title": "Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.", "content": "Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence.", "contents": "Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin. Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence."}
{"id": "PMID:1096306", "title": "Studies on coagulation and fibrinolysis in cases of Falciparum malaria.", "content": "Coagulation and fibrinolytic studies were conducted in 18 cases of severe falciparum malaria including cases with parasitaemia above 5% and with pernicious manifestations such as coma, jaundice, anuria, pulmonary oedema, bleeding tendency, etc., irrespective of parasitaemia. Marked changes in blood coagulograms and high levels of serum fibrin degradation products appeared only in cases with very severe cerebral involvement and also in cases with very high parasitaemia alone. These investigations indicated that intravascular coagulation occurs only in patients suffering from falciparum malaira who develop cerebral manifestations and in cases with high parasitaemia.", "contents": "Studies on coagulation and fibrinolysis in cases of Falciparum malaria. Coagulation and fibrinolytic studies were conducted in 18 cases of severe falciparum malaria including cases with parasitaemia above 5% and with pernicious manifestations such as coma, jaundice, anuria, pulmonary oedema, bleeding tendency, etc., irrespective of parasitaemia. Marked changes in blood coagulograms and high levels of serum fibrin degradation products appeared only in cases with very severe cerebral involvement and also in cases with very high parasitaemia alone. These investigations indicated that intravascular coagulation occurs only in patients suffering from falciparum malaira who develop cerebral manifestations and in cases with high parasitaemia."}
{"id": "PMID:1096326", "title": "Maintenance haemodialysis and renal transplantation facilities in South Africa-July 1974.", "content": "A survey of facilities available in South Africa for patients suffering from chronic renal failure was made in July 1974. This showed that 14 maintenance haemodialysis centres had a total of 59 dialysing stations with a capacity for treating 154 patients. Five centres had established programmes for renal transplantation. The total number of renal failure survivors on treatment included 129 patients on maintenance dialysis and 177 with grafts. This represents a 31% increase in the number of patients on treatment since a similar survey was made in July 1973.", "contents": "Maintenance haemodialysis and renal transplantation facilities in South Africa-July 1974. A survey of facilities available in South Africa for patients suffering from chronic renal failure was made in July 1974. This showed that 14 maintenance haemodialysis centres had a total of 59 dialysing stations with a capacity for treating 154 patients. Five centres had established programmes for renal transplantation. The total number of renal failure survivors on treatment included 129 patients on maintenance dialysis and 177 with grafts. This represents a 31% increase in the number of patients on treatment since a similar survey was made in July 1973."}
{"id": "PMID:1096327", "title": "History of mental health services in South Africa. Part VII. Services since union.", "content": "The main task of the mental health service since Union has been to overcome a chronic shortage of accommodation. The main reason for the lack of success was financial. The strain on accommodation was partially relieved by the introduction of modern methods of treatment and by the erection of hospitals for Blacks in the Homelands. The new Mental Health ACT OF 1973 is an improvement on the Mental Disorders Act of 1916.", "contents": "History of mental health services in South Africa. Part VII. Services since union. The main task of the mental health service since Union has been to overcome a chronic shortage of accommodation. The main reason for the lack of success was financial. The strain on accommodation was partially relieved by the introduction of modern methods of treatment and by the erection of hospitals for Blacks in the Homelands. The new Mental Health ACT OF 1973 is an improvement on the Mental Disorders Act of 1916."}
{"id": "PMID:1096328", "title": "Penetrating wounds of the heart and great vessels. Experience with 24 cases including 3 with intracardiac defects.", "content": "Twenty-four cases of penetrating cardiac injuries due to stab wounds, which were seen over a 3-year period, are reviewed. Ten patients were treated by aspiration alone because tamponade was mild, or because they were seen more than 12 hours after injury. One patient with multiple stab wounds in the ventricles and left atrium died during emergency thoracotomy in the admission room. The mortality rate of 4,5% is considerably lower than rates reported in other series. Two cases of traumatic ventricular septal defects, one case of traumatic aortic incompetence and sinus of Valsalva fistulae with rupture into the right ventricle and right atrium, and one case of ascending aorta-innominate vein fistula are discussed. Our present plan of management is outlined.", "contents": "Penetrating wounds of the heart and great vessels. Experience with 24 cases including 3 with intracardiac defects. Twenty-four cases of penetrating cardiac injuries due to stab wounds, which were seen over a 3-year period, are reviewed. Ten patients were treated by aspiration alone because tamponade was mild, or because they were seen more than 12 hours after injury. One patient with multiple stab wounds in the ventricles and left atrium died during emergency thoracotomy in the admission room. The mortality rate of 4,5% is considerably lower than rates reported in other series. Two cases of traumatic ventricular septal defects, one case of traumatic aortic incompetence and sinus of Valsalva fistulae with rupture into the right ventricle and right atrium, and one case of ascending aorta-innominate vein fistula are discussed. Our present plan of management is outlined."}
{"id": "PMID:1096329", "title": "Clinical experience with a synthetic absorbable surgical suture.", "content": "The Vicryl suture was assessed in 72 surgical patients who underwent follow-up observation for at least 30 days, and it proved to be an excellent inert absorbable synthetic suture.", "contents": "Clinical experience with a synthetic absorbable surgical suture. The Vicryl suture was assessed in 72 surgical patients who underwent follow-up observation for at least 30 days, and it proved to be an excellent inert absorbable synthetic suture."}
{"id": "PMID:1096338", "title": "Metabolic rate in acute respiratory failure complicating sepsis.", "content": "A simple, reproducible, and noninvasive method was developed for measuring metabolic rate in patients receiving assisted ventilation and increased concentrations of inspired O2. In nonseptic patients requiring assisted ventilation, metabolic rate was within the predicted normal range, but in septic patients it was increased by an average of 60 percent over the predicted normal. This level of resting hypermetabolism is greater than previously reported in septic patients not requiring assisted ventilation. The explanation of this difference is likely to be related to the greater severity of the illness in the present group. The potential clinical significance of these findings is discussed.", "contents": "Metabolic rate in acute respiratory failure complicating sepsis. A simple, reproducible, and noninvasive method was developed for measuring metabolic rate in patients receiving assisted ventilation and increased concentrations of inspired O2. In nonseptic patients requiring assisted ventilation, metabolic rate was within the predicted normal range, but in septic patients it was increased by an average of 60 percent over the predicted normal. This level of resting hypermetabolism is greater than previously reported in septic patients not requiring assisted ventilation. The explanation of this difference is likely to be related to the greater severity of the illness in the present group. The potential clinical significance of these findings is discussed."}
{"id": "PMID:1096339", "title": "Notes on the handling of ultrafine suture material.", "content": "For the convenient handling of microsurgical suture materials, suitable forceps are needed and these must be finely adjusted and well cared for. Only a short length of thread should be used on the needle. Difficulties arise in picking up the needle, in making a loop in the thread, in keeping the loop on the forceps while tying, and in picking up the end of the thread. Simple methods of avoiding entanglement and exasperation in these situations are described.", "contents": "Notes on the handling of ultrafine suture material. For the convenient handling of microsurgical suture materials, suitable forceps are needed and these must be finely adjusted and well cared for. Only a short length of thread should be used on the needle. Difficulties arise in picking up the needle, in making a loop in the thread, in keeping the loop on the forceps while tying, and in picking up the end of the thread. Simple methods of avoiding entanglement and exasperation in these situations are described."}
{"id": "PMID:1096340", "title": "Effect of antithymocyte globulin and other immune reactants on human platelets.", "content": "Human platelets suspended in autologous plasma do not respond to nonspecific immune complexes as do platelet suspensions from rabbits and dogs. However, platelets of all three species do undergo aggregation and the release reaction when exposed to antibodies directed against platelets. Antithymocyte globulin (ATG) contains such antibodies, apparently because of antigens common to both thymocytes and platelets. ATG-induced platelet aggregation and release is thus a specific reaction which may be responsible for the thrombocytopenia and thrombotic complications occasionally seen following the administration of ATG. However, if ATG is given properly, its effect on platelets should not constitute a contraindication to the use of this immunosuppressive drug. Since nonspecific immune complexes do not affect human platelets in the presence of plasma, it would appear that platelet aggregates seen in hyperacute and acute rejection result from endothelial damage rather than an effect of immune complexes on platelets.", "contents": "Effect of antithymocyte globulin and other immune reactants on human platelets. Human platelets suspended in autologous plasma do not respond to nonspecific immune complexes as do platelet suspensions from rabbits and dogs. However, platelets of all three species do undergo aggregation and the release reaction when exposed to antibodies directed against platelets. Antithymocyte globulin (ATG) contains such antibodies, apparently because of antigens common to both thymocytes and platelets. ATG-induced platelet aggregation and release is thus a specific reaction which may be responsible for the thrombocytopenia and thrombotic complications occasionally seen following the administration of ATG. However, if ATG is given properly, its effect on platelets should not constitute a contraindication to the use of this immunosuppressive drug. Since nonspecific immune complexes do not affect human platelets in the presence of plasma, it would appear that platelet aggregates seen in hyperacute and acute rejection result from endothelial damage rather than an effect of immune complexes on platelets."}
{"id": "PMID:1096341", "title": "Recognition and treatment of pathologic fractures in the nineteenth century.", "content": "The spread of cancer to the skeletal system was first clearly recognized in the 19th century, and accounts of pathologic fractures appear in many clinical and pathologic reports published during that period. Noteworthy descriptions included fractures observed in both living patients and in patients who died of cancer. The problems considered by the 19th century physicians included the still timely areas of clinical diagnosis, prevention of fracture, the occurrence of prefracture pain, and treatment by immobilization or operation.", "contents": "Recognition and treatment of pathologic fractures in the nineteenth century. The spread of cancer to the skeletal system was first clearly recognized in the 19th century, and accounts of pathologic fractures appear in many clinical and pathologic reports published during that period. Noteworthy descriptions included fractures observed in both living patients and in patients who died of cancer. The problems considered by the 19th century physicians included the still timely areas of clinical diagnosis, prevention of fracture, the occurrence of prefracture pain, and treatment by immobilization or operation."}
{"id": "PMID:1096342", "title": "Effect of three proteinaceous foreign materials on infected and subinfected wound models.", "content": "A model was devised to determine the effects of several foreign bodies (microcrystalline collagen hemostat (MCCH), blood clot, catgut suture) on S. aureus wound contamination and wound infection. In a subinfected wound model, MCCH, when compared with the other foreign bodies, showed an increase in the number of wounds classified as \"contaminated,\" but 84 percent of these wounds yielded bacterial counts below the level accepted for biologically significant wound infection (10 to the 5th power bacteria per gram tissue). The number of \"infected\" wounds was not significantly greater than the number found in the uninoculated control group. In the infected wound model, all groups of inoculated animals showed significant infections. The severity of the wound infection was unaffected by the presence of catgut suture, clotted blood, or MCCH. Higher levels were not seen for the MCCH animals than for the inoculated control animals. We did not see in these experimental wound studies with MCCH a significant aggravation of wound contamination or wound infection during the 48 hour period following inoculation with varying dose levels of S. aureus. Further studies would be necessary to determine the possible bacteriological response after 48 hours.", "contents": "Effect of three proteinaceous foreign materials on infected and subinfected wound models. A model was devised to determine the effects of several foreign bodies (microcrystalline collagen hemostat (MCCH), blood clot, catgut suture) on S. aureus wound contamination and wound infection. In a subinfected wound model, MCCH, when compared with the other foreign bodies, showed an increase in the number of wounds classified as \"contaminated,\" but 84 percent of these wounds yielded bacterial counts below the level accepted for biologically significant wound infection (10 to the 5th power bacteria per gram tissue). The number of \"infected\" wounds was not significantly greater than the number found in the uninoculated control group. In the infected wound model, all groups of inoculated animals showed significant infections. The severity of the wound infection was unaffected by the presence of catgut suture, clotted blood, or MCCH. Higher levels were not seen for the MCCH animals than for the inoculated control animals. We did not see in these experimental wound studies with MCCH a significant aggravation of wound contamination or wound infection during the 48 hour period following inoculation with varying dose levels of S. aureus. Further studies would be necessary to determine the possible bacteriological response after 48 hours."}
{"id": "PMID:1096344", "title": "Renal autotransplantation and partial resection of the ureter for recurrent benign ureteral tumor.", "content": "A report of a patient treated by partial resection of the ureter and renal autotransplantation for recurrent benign ureteral tumors and impaired renal function in the contralateral kidney is presented. Autotransplantation of the ipsilateral kidney associated with benign disease of the renal excretory system is recommended even with normal function of the contralateral kidney. Experience with renal homotransplantation has led to excellent technical and functional results from autotransplantation, and use of the procedure is expanding to include treatment of a variety of renal, ureteral, and renovascular pathology. The role of renal autotransplantation in the preservation of functioning renal units is emphasized.", "contents": "Renal autotransplantation and partial resection of the ureter for recurrent benign ureteral tumor. A report of a patient treated by partial resection of the ureter and renal autotransplantation for recurrent benign ureteral tumors and impaired renal function in the contralateral kidney is presented. Autotransplantation of the ipsilateral kidney associated with benign disease of the renal excretory system is recommended even with normal function of the contralateral kidney. Experience with renal homotransplantation has led to excellent technical and functional results from autotransplantation, and use of the procedure is expanding to include treatment of a variety of renal, ureteral, and renovascular pathology. The role of renal autotransplantation in the preservation of functioning renal units is emphasized."}
{"id": "PMID:1096345", "title": "Pyloric reflux gastritis: the offending agent.", "content": "Tubes of canine gastric corpus were constructed so that the mucosa was exposed chronically to jejunal contents, pancreatic juice, or bile. Biopsies of these mucosae obtained 4 to 24 months later were compared with each other and with the normal stomach for parietal cell numbers, mucus cell numbers, inflammation, glandular disorder, and surface-cell hyperplasia. All three preparations showed marked gastrititis, but whole jejunal contents caused more severe changes than did either pancreatic juice or bile, which were approximately equal.", "contents": "Pyloric reflux gastritis: the offending agent. Tubes of canine gastric corpus were constructed so that the mucosa was exposed chronically to jejunal contents, pancreatic juice, or bile. Biopsies of these mucosae obtained 4 to 24 months later were compared with each other and with the normal stomach for parietal cell numbers, mucus cell numbers, inflammation, glandular disorder, and surface-cell hyperplasia. All three preparations showed marked gastrititis, but whole jejunal contents caused more severe changes than did either pancreatic juice or bile, which were approximately equal."}
{"id": "PMID:1096346", "title": "Computerization of the surgical intensive care unit: improvement of patient care via education.", "content": "For the past 18 months we have been evaluating and developing a computerized patient-monitoring system in our surgical intensive care unit. Despite the enormous potential for use of such systems, we have been impressed with its underutilization and its failure to yield objective improvement in patient care at our institution and many others. The output of the system was ignored because the decision maker was unable or unwilling to integrate the more sophisticated data presented to him. The computer was relegated to the position of \"redundant secretary\". In an attempt to reverse this situation, we have developed a multilevel, multigoal educational system employing the computer. We have implemented brief educational programs for use by all unit personnel to explain deviant monitored variables. Given a physiologic subsystem and a particular variable, personnel can: (1) inquire whether or not the variable is deviant; (2) obtain a list of probable causes for the deviation; (3) obtain an explanation of the pathophysiology of particular deviants as well as instruction on how to identify a most probable cause; and (4) inquire how to correct specific deviants. When we monitored the system utilization after implementation of the educational programs, we found all of the system had improved utilization. As a result we have a better educated staff who communicate more effectively, deal with more sophisticated information, and make better decisions with resultant improved patient care. Additionally, the staff is eager to help improve the system. We believe the full potential of such systems can be obtained only through education.", "contents": "Computerization of the surgical intensive care unit: improvement of patient care via education. For the past 18 months we have been evaluating and developing a computerized patient-monitoring system in our surgical intensive care unit. Despite the enormous potential for use of such systems, we have been impressed with its underutilization and its failure to yield objective improvement in patient care at our institution and many others. The output of the system was ignored because the decision maker was unable or unwilling to integrate the more sophisticated data presented to him. The computer was relegated to the position of \"redundant secretary\". In an attempt to reverse this situation, we have developed a multilevel, multigoal educational system employing the computer. We have implemented brief educational programs for use by all unit personnel to explain deviant monitored variables. Given a physiologic subsystem and a particular variable, personnel can: (1) inquire whether or not the variable is deviant; (2) obtain a list of probable causes for the deviation; (3) obtain an explanation of the pathophysiology of particular deviants as well as instruction on how to identify a most probable cause; and (4) inquire how to correct specific deviants. When we monitored the system utilization after implementation of the educational programs, we found all of the system had improved utilization. As a result we have a better educated staff who communicate more effectively, deal with more sophisticated information, and make better decisions with resultant improved patient care. Additionally, the staff is eager to help improve the system. We believe the full potential of such systems can be obtained only through education."}
{"id": "PMID:1096351", "title": "Measurement of cardiac output by thermal dilution in infants and children.", "content": "The accuracy of the thermal dilution technique for measuring cardiac output in infants and children has been assessed by comparison with the direct Fick technique. Satisfactory correlation between the two methods has been demonstrated, and it is concluded that a commercially available monitor can be used to determine cardiac output by thermal dilution in acutely sick infants and children. The volume of injectate should be selected according to the weight of the child, and the value displayed by the monitor should be adjusted by the appropriate fraction.", "contents": "Measurement of cardiac output by thermal dilution in infants and children. The accuracy of the thermal dilution technique for measuring cardiac output in infants and children has been assessed by comparison with the direct Fick technique. Satisfactory correlation between the two methods has been demonstrated, and it is concluded that a commercially available monitor can be used to determine cardiac output by thermal dilution in acutely sick infants and children. The volume of injectate should be selected according to the weight of the child, and the value displayed by the monitor should be adjusted by the appropriate fraction."}
{"id": "PMID:1096352", "title": "Intravenous prednisolone in chronic bronchial asthma.", "content": "A single injection of 40 mg prednisolone phosphate was given to 10 patients with chronic bronchial asthma. Changes in pulmonary function were followed over a 30-hour period. Statistically significant changes occurred in the tests employed one hour after the injection of prednisolone. The maximum change for the group as a whole was seen to occur after eight hours. This time course of response is very similar to that obtained in previous studies on similar groups of patients with oral prednisolone where the peak effect occurred nine hours after the drug had been given. Intravenous hydrocortisone produces a much earlier peak effect, at five hours, when it is administered to chronic asthmatic patients.", "contents": "Intravenous prednisolone in chronic bronchial asthma. A single injection of 40 mg prednisolone phosphate was given to 10 patients with chronic bronchial asthma. Changes in pulmonary function were followed over a 30-hour period. Statistically significant changes occurred in the tests employed one hour after the injection of prednisolone. The maximum change for the group as a whole was seen to occur after eight hours. This time course of response is very similar to that obtained in previous studies on similar groups of patients with oral prednisolone where the peak effect occurred nine hours after the drug had been given. Intravenous hydrocortisone produces a much earlier peak effect, at five hours, when it is administered to chronic asthmatic patients."}
{"id": "PMID:1096353", "title": "Growth and development of human fetal trachea and lung in immune-deprived mice.", "content": "Trachea and lung parenchyma from human fetuses were transplanted subcutaneously into immune-deprived mice. After seven to 10 weeks they were removed for histological examination. It was found that the tissues continued to grow and develop along similar lines to those which they would have followed in utero.", "contents": "Growth and development of human fetal trachea and lung in immune-deprived mice. Trachea and lung parenchyma from human fetuses were transplanted subcutaneously into immune-deprived mice. After seven to 10 weeks they were removed for histological examination. It was found that the tissues continued to grow and develop along similar lines to those which they would have followed in utero."}
{"id": "PMID:1096359", "title": "[Problems of resistance (author's transl)].", "content": "The results of sensitivity tests performed during the period from 1970 up to 1973 inclusive are compared with those obtained in pervious years. This showed that resistance to penicillin had increased in staphylococci causing bovine mastitis. During each single year, however, there was a decrease. S. typhimurium isolated from cattle showed increased resistance to chloramphenicol, neomycin and ampicillin; S. dublin showed an increase in resistance to chloramphenicol. E. coli isolated from calves with coli-bacillosis had become less sensitive to all the antibiotics studied. Pathogenic strains of E. coli isolated from swine also showed increased resistance, among others to tetracycline, neomycin and ampicillin. The danger constituted by this development and the factthat comparison with the results obtained by other presents difficulties in some cases are stressed.", "contents": "[Problems of resistance (author's transl)]. The results of sensitivity tests performed during the period from 1970 up to 1973 inclusive are compared with those obtained in pervious years. This showed that resistance to penicillin had increased in staphylococci causing bovine mastitis. During each single year, however, there was a decrease. S. typhimurium isolated from cattle showed increased resistance to chloramphenicol, neomycin and ampicillin; S. dublin showed an increase in resistance to chloramphenicol. E. coli isolated from calves with coli-bacillosis had become less sensitive to all the antibiotics studied. Pathogenic strains of E. coli isolated from swine also showed increased resistance, among others to tetracycline, neomycin and ampicillin. The danger constituted by this development and the factthat comparison with the results obtained by other presents difficulties in some cases are stressed."}
{"id": "PMID:1096360", "title": "[A case of cutaneous actinomycosis (streptothricosis) in cattle, caused by dermatophilus congolensis (author's transl)].", "content": "A skin condition in cattle, associated with crusted lesions on the back, flanks, neck, head and skin of the udder was found to be cutaneous actinomycosis (streptothricosis) caused by D. congolensis, which is common in tropical regions during the rainy season. This actinomycete was isolated for the first time from cattle in the Netherlands in the case reported. Data culled from the literature as well as the diagnosis and treatment of the patient are discussed.", "contents": "[A case of cutaneous actinomycosis (streptothricosis) in cattle, caused by dermatophilus congolensis (author's transl)]. A skin condition in cattle, associated with crusted lesions on the back, flanks, neck, head and skin of the udder was found to be cutaneous actinomycosis (streptothricosis) caused by D. congolensis, which is common in tropical regions during the rainy season. This actinomycete was isolated for the first time from cattle in the Netherlands in the case reported. Data culled from the literature as well as the diagnosis and treatment of the patient are discussed."}
{"id": "PMID:1096362", "title": "[The femoro-crural venous bypass in chronic arterial block of the leg (author's transl)].", "content": "A report on 58 reconstructive vascular operations in chronic blocks of arteries of the leg. 4 different types of block in these arteries can be distinguished. Operation is indicated only in severe disturbances of circulation of stage III and IV. The criterion for reconstruction is not found in the proximal part of the arteries. Angiographic proof of at least one leg-artery down to the periphery being patent and permitting free flow is required. The various techniques applicable to each type of block, early and late results are reported on. Suring a period of up to 3 years block recurred in 26%. Most recurrences (46%) were seen within the first 30 days.", "contents": "[The femoro-crural venous bypass in chronic arterial block of the leg (author's transl)]. A report on 58 reconstructive vascular operations in chronic blocks of arteries of the leg. 4 different types of block in these arteries can be distinguished. Operation is indicated only in severe disturbances of circulation of stage III and IV. The criterion for reconstruction is not found in the proximal part of the arteries. Angiographic proof of at least one leg-artery down to the periphery being patent and permitting free flow is required. The various techniques applicable to each type of block, early and late results are reported on. Suring a period of up to 3 years block recurred in 26%. Most recurrences (46%) were seen within the first 30 days."}
{"id": "PMID:1096363", "title": "[Successful surgical correction of total anomalous pulmonary venous drainage into the portal vein in a ten days old infant (author's transl)].", "content": "An infant, nine days old, with total anomalous pulmonary venous drainage into the portal vein was examined. The exact anatomy was clarified by means of cardiac catheterization and angiocardiography. One day later the operation was performed utilizing circulatory arrest in deep hypothermia. The procedure was successful, and the child, two years old now, is doing well. The problems of examination, treatment and surgery are discussed. There are only a few cases with successful correction in the literature.", "contents": "[Successful surgical correction of total anomalous pulmonary venous drainage into the portal vein in a ten days old infant (author's transl)]. An infant, nine days old, with total anomalous pulmonary venous drainage into the portal vein was examined. The exact anatomy was clarified by means of cardiac catheterization and angiocardiography. One day later the operation was performed utilizing circulatory arrest in deep hypothermia. The procedure was successful, and the child, two years old now, is doing well. The problems of examination, treatment and surgery are discussed. There are only a few cases with successful correction in the literature."}
{"id": "PMID:1096371", "title": "Agar-screew specimen carrier for bulk processing of biopsy material for electron microscopy.", "content": "A specimen carrier for processing large numbers of biopsy materials for epoxy embedding and electron microscopy is described. Commercially available  18-mesh stainless steel or 16-mesh aluminum wire screening is used. The screening is cut into 1 x 3-inch strips. One corner is snipped off for orientation purposes. Four drops of warm 4% agar is placed on a prewarmed standard microscopic glass slide. A thin agar support film is formed on the bottom side of the horizontally held wire screen by lightly running it against the agar. Tissue blocks trimmed to 1 mm-3 are blotted on filter paper and placed in a prearranged order on the top surface of the support film. A thin top coating of agar is applied on the specimen by touching it with the tip of a pasteur pipette containing warm 4% agar. The agar-screen unit with the mounted specimens is stabilized in 4% buffered formalin and rinsed with Sorenson's phosphate buffer, pH 7.4, with 6.8% sucrose. It is then processed as a unit through routine osmium tetroxide postfixation, alcohol dehydration, and Epon 812 filtration. The tissue blocks are plucked off the agar support film with fine-tipped tweezers and embedded in individual capsules. No difficulty in thin sectioning was encountered and examination of the sections under the electron microscope showed good infiltration by the epoxy resin.", "contents": "Agar-screew specimen carrier for bulk processing of biopsy material for electron microscopy. A specimen carrier for processing large numbers of biopsy materials for epoxy embedding and electron microscopy is described. Commercially available  18-mesh stainless steel or 16-mesh aluminum wire screening is used. The screening is cut into 1 x 3-inch strips. One corner is snipped off for orientation purposes. Four drops of warm 4% agar is placed on a prewarmed standard microscopic glass slide. A thin agar support film is formed on the bottom side of the horizontally held wire screen by lightly running it against the agar. Tissue blocks trimmed to 1 mm-3 are blotted on filter paper and placed in a prearranged order on the top surface of the support film. A thin top coating of agar is applied on the specimen by touching it with the tip of a pasteur pipette containing warm 4% agar. The agar-screen unit with the mounted specimens is stabilized in 4% buffered formalin and rinsed with Sorenson's phosphate buffer, pH 7.4, with 6.8% sucrose. It is then processed as a unit through routine osmium tetroxide postfixation, alcohol dehydration, and Epon 812 filtration. The tissue blocks are plucked off the agar support film with fine-tipped tweezers and embedded in individual capsules. No difficulty in thin sectioning was encountered and examination of the sections under the electron microscope showed good infiltration by the epoxy resin."}
{"id": "PMID:1096378", "title": "Anaemia in mice with concomitant Schistosoma mansoni and Plasmodium berghei yoelii infection.", "content": "1. The effect on anaemia in mice given Plasmodium berghei yoelii 3 and 5 weeks after exposure to Schistosoma mansoni cercariae, was investigated. 2. Haematological criteria (PCV and haemoglobin levels), reticulocytosis, parasitaemia and splenomegaly were used as indices. 3. Anaemia was severe in the animals given P. b. yoelii and in those with mixed infection (P. b: yoelii plus S. mansoni). Malaria was found to dominate the picture until the clearance of the parasitaemia. The effect of the interaction between the diseases on the anaemia was nil. 4. Toward the end of the experiment, moderate splenomegaly was observed in the mice with mixed infection.", "contents": "Anaemia in mice with concomitant Schistosoma mansoni and Plasmodium berghei yoelii infection. 1. The effect on anaemia in mice given Plasmodium berghei yoelii 3 and 5 weeks after exposure to Schistosoma mansoni cercariae, was investigated. 2. Haematological criteria (PCV and haemoglobin levels), reticulocytosis, parasitaemia and splenomegaly were used as indices. 3. Anaemia was severe in the animals given P. b. yoelii and in those with mixed infection (P. b: yoelii plus S. mansoni). Malaria was found to dominate the picture until the clearance of the parasitaemia. The effect of the interaction between the diseases on the anaemia was nil. 4. Toward the end of the experiment, moderate splenomegaly was observed in the mice with mixed infection."}
{"id": "PMID:1096379", "title": "The rapid isolation from human blood of concentrated, white-cell-free preparations of Plasmodium falciparum.", "content": "The sucrose gradient centrifugation method has been applied to representative samples of human (Gambian) blood infected with ring, trophozoite, schizont and gametocyte stages of P. falciparum in order to assess quantitatively the efficiency of recovery, white cell removal and the degree of enrichment of the infected cell fraction. Maximal white cell removal was 90%. (a.v.) Average infected cell recoveries varied with the level of white cell contamination, namely 47% (2-5% WBC), 34% (1-2% WBC) and 24% (smaller than 1% WBC); infected cells were enriched 2-9-fold on average, and up to 22-fold in the case of gametocytes. Preliminary attempts to prepare free parasites by nitrogen cavitation of infected cells showed that disruption took place at much lower pressures than those required to break normal red cells. Gel diffusion analyses showed that the most highly purified infected cell preparations retained the full precipitinogenic spectrum of the original crude preparation.", "contents": "The rapid isolation from human blood of concentrated, white-cell-free preparations of Plasmodium falciparum. The sucrose gradient centrifugation method has been applied to representative samples of human (Gambian) blood infected with ring, trophozoite, schizont and gametocyte stages of P. falciparum in order to assess quantitatively the efficiency of recovery, white cell removal and the degree of enrichment of the infected cell fraction. Maximal white cell removal was 90%. (a.v.) Average infected cell recoveries varied with the level of white cell contamination, namely 47% (2-5% WBC), 34% (1-2% WBC) and 24% (smaller than 1% WBC); infected cells were enriched 2-9-fold on average, and up to 22-fold in the case of gametocytes. Preliminary attempts to prepare free parasites by nitrogen cavitation of infected cells showed that disruption took place at much lower pressures than those required to break normal red cells. Gel diffusion analyses showed that the most highly purified infected cell preparations retained the full precipitinogenic spectrum of the original crude preparation."}
{"id": "PMID:1096384", "title": "[Ecology of Culex pipiens fatigans larvae in an area of high endemicity of Bancroftian filariasis (author's transl)].", "content": "Culex pipiens fatigans in the main vector of bancroftian filariasis in the Mayotte island (Comores) where it imposes an important health problem. The breeding-sites of C. p. fatigans are either man-made (latrines, cesspools, various containers), or natural (polluted water of estuaries of some rivers). The man-made breeding-sites are not similarly distributed in the different localities of the island. Their distribution varies according to the customs of the inhabitants, size and site of the community. They are mainly represented by latrines, where Anjouan ethnic group is predominent; by cesspools in localities inhabited by Sakalava (a Malagasian ethnic group) and by other latrines and cesspools in mahoraises (inhabitants of Mayotte) and cosmopolitan localities. In rural areas, the percentage of habitations with cesspools usually increases with the size of the villages. The various breeding-sites are primarily productive when they are built on a ground with poor permeability. The importance of the present information is further discussed with relation to the population dynamics of C.p. fatigans and to the measures which may be useful for a control program of this mosquito.", "contents": "[Ecology of Culex pipiens fatigans larvae in an area of high endemicity of Bancroftian filariasis (author's transl)]. Culex pipiens fatigans in the main vector of bancroftian filariasis in the Mayotte island (Comores) where it imposes an important health problem. The breeding-sites of C. p. fatigans are either man-made (latrines, cesspools, various containers), or natural (polluted water of estuaries of some rivers). The man-made breeding-sites are not similarly distributed in the different localities of the island. Their distribution varies according to the customs of the inhabitants, size and site of the community. They are mainly represented by latrines, where Anjouan ethnic group is predominent; by cesspools in localities inhabited by Sakalava (a Malagasian ethnic group) and by other latrines and cesspools in mahoraises (inhabitants of Mayotte) and cosmopolitan localities. In rural areas, the percentage of habitations with cesspools usually increases with the size of the villages. The various breeding-sites are primarily productive when they are built on a ground with poor permeability. The importance of the present information is further discussed with relation to the population dynamics of C.p. fatigans and to the measures which may be useful for a control program of this mosquito."}
{"id": "PMID:1096385", "title": "[Chemotherapeutic studies on litomosoides carinii infection of mastomys natalensis. 5. Alterations of haematological parameters after the administration of filaricidal compounds (author's transl)].", "content": "The peripheral blood picture of multimammate rats (Mastomys natalensis) infected with Litomosoides carinii was studied. Changes were followed in patent infections from 64 to 176 days after infection in animals treated with diethylcarbamazine (5 x 100 mg/kg per os), HOE 258 V (5 X 10 mg/kg subcutaneously), suramin (5 x 40 mg/kg subcutaneously) and with a combined treatment of diethylcarbamazine and suramin. In addition, haematological changes were followed in non-infected animals treated with suramin alone as well as in non-infected and infected animals receiving no treatment. During the course of non-treated L. carinii infection in Mastomys natalensis, animals developed anaemia, an increased sedimentation rate and a leucopenia. In addition there was a relative increase in circulating eosinophils, in large lymphocytes and monocytes and a relative decrease in small lymphocytes. After treatment with the microfilaricidal drugs diethylcarbamazine and HOE 258 V blood changes followed those of infection controls but there was a transient change towards normal in peripheral blood values when the microfilariaemia was reduced by the drugs. After treatment with the macrofilaricide suramin, nearly normal values were reached at the end of the experiment for the red blood cell and leucocytes levels. Combined treatment with suramin and diethylcarbamazine resulted in relatively normal levels for some parameters. Suramin was shown to cause haematological changes in normal animals which became evident several days before use of the drug. Such changes added to the pathological alterations caused by the filarial infection. In both infected and non-infected animals suramin treatment resulted in oedema around the nose and on the front feet approximately three weeks afterwards.", "contents": "[Chemotherapeutic studies on litomosoides carinii infection of mastomys natalensis. 5. Alterations of haematological parameters after the administration of filaricidal compounds (author's transl)]. The peripheral blood picture of multimammate rats (Mastomys natalensis) infected with Litomosoides carinii was studied. Changes were followed in patent infections from 64 to 176 days after infection in animals treated with diethylcarbamazine (5 x 100 mg/kg per os), HOE 258 V (5 X 10 mg/kg subcutaneously), suramin (5 x 40 mg/kg subcutaneously) and with a combined treatment of diethylcarbamazine and suramin. In addition, haematological changes were followed in non-infected animals treated with suramin alone as well as in non-infected and infected animals receiving no treatment. During the course of non-treated L. carinii infection in Mastomys natalensis, animals developed anaemia, an increased sedimentation rate and a leucopenia. In addition there was a relative increase in circulating eosinophils, in large lymphocytes and monocytes and a relative decrease in small lymphocytes. After treatment with the microfilaricidal drugs diethylcarbamazine and HOE 258 V blood changes followed those of infection controls but there was a transient change towards normal in peripheral blood values when the microfilariaemia was reduced by the drugs. After treatment with the macrofilaricide suramin, nearly normal values were reached at the end of the experiment for the red blood cell and leucocytes levels. Combined treatment with suramin and diethylcarbamazine resulted in relatively normal levels for some parameters. Suramin was shown to cause haematological changes in normal animals which became evident several days before use of the drug. Such changes added to the pathological alterations caused by the filarial infection. In both infected and non-infected animals suramin treatment resulted in oedema around the nose and on the front feet approximately three weeks afterwards."}
{"id": "PMID:1096387", "title": "[Cytogenetic characteristics of suckling mouse brain cell cultures, chronically infected with Japanese encephalitis virus].", "content": "The brain cells of the suckling mice line MSB-1-K-33 chronically infected by an attenuated variant of the Japanese encephalitis virus and of cell clones isolated from the later, had mainly a neartetraploid keryotype (the modal class 70-71 chromosomes). In metaphases of cloned cell populations, an increase of number of the chromosomes was especially obvious in clones 3 and 4 (modal classes in both cases were 76-77 chromosomes). Cell population of clone 1 differed insignificantly from that of the parental line MSB-1-K-33 in respect of its cytogenetic characteristics. In metaphases of the line MSB-1-K-33 and clones 1, 3, and 4, a high frequency of chromosomal damages was observed. The most frequent type of structural chromosome aberrations were chromatid breaks. The karyotypes of clones 3 and 4 were characterized by presence of a large marker metacentric chromosome in 50-53% of the cells. The data obtained suggest a continuous effect of the Japanese encephalitis virus on the karyotype of cells in infected culture.", "contents": "[Cytogenetic characteristics of suckling mouse brain cell cultures, chronically infected with Japanese encephalitis virus]. The brain cells of the suckling mice line MSB-1-K-33 chronically infected by an attenuated variant of the Japanese encephalitis virus and of cell clones isolated from the later, had mainly a neartetraploid keryotype (the modal class 70-71 chromosomes). In metaphases of cloned cell populations, an increase of number of the chromosomes was especially obvious in clones 3 and 4 (modal classes in both cases were 76-77 chromosomes). Cell population of clone 1 differed insignificantly from that of the parental line MSB-1-K-33 in respect of its cytogenetic characteristics. In metaphases of the line MSB-1-K-33 and clones 1, 3, and 4, a high frequency of chromosomal damages was observed. The most frequent type of structural chromosome aberrations were chromatid breaks. The karyotypes of clones 3 and 4 were characterized by presence of a large marker metacentric chromosome in 50-53% of the cells. The data obtained suggest a continuous effect of the Japanese encephalitis virus on the karyotype of cells in infected culture."}
{"id": "PMID:1096388", "title": "A comparative study of daily followed by twice or once weekly regimens of ethambutol and rifampicin in retreatment of patients with pulmonary tuberculosis. The results at 1 year. A cooperative tuberculosis chemotherapy study in Poland.", "content": "The present report concerns the results at 1 year of a co-operative controlled clinical study carried out in Poland of the retreatment of patients with active, chronic, polyresistant far-advance pulmonary tuberculosis with an oral regimen of daily followed by intermittent ethambutol and rifampicin. A comparison was made of once- and twice-weekly supervised intermittent regimens of rifampicin 1200 mg plus ethambutol 50 mg/kg body weight under out-patient conditions after an initial inpatient phase of rifampicin 600 mg and ethambutol 25 mg/kg daily for 12 weeks. Patients were allocated at random to the regimens. Of 247 patients admitted to the study, 201 (81 per cent) completed 1 year's treatment as prescribed by the protocol, 46 (19 per cent) patients terminated their treatment prematurely before 1 year. After the daily phase of 12 weeks' treatment, 82 per cent were negative on smear and 85 per cent on culture; in the continuation intermittent phase, 98 per cent of patients in the once-weekly (E1R1) regimen were negative on culture at 28 weeks and 98 per cent in the twice-weekly (E2R2) regimen. The corresponding proportions at 52 weeks were 97 per cent and 97 per cent. At 12 months, 96 per cent of 101 ER/E1R1 and 96 per cent of 100 ER/E2R2 patients who completed 1 years' treatment were culture-negative.", "contents": "A comparative study of daily followed by twice or once weekly regimens of ethambutol and rifampicin in retreatment of patients with pulmonary tuberculosis. The results at 1 year. A cooperative tuberculosis chemotherapy study in Poland. The present report concerns the results at 1 year of a co-operative controlled clinical study carried out in Poland of the retreatment of patients with active, chronic, polyresistant far-advance pulmonary tuberculosis with an oral regimen of daily followed by intermittent ethambutol and rifampicin. A comparison was made of once- and twice-weekly supervised intermittent regimens of rifampicin 1200 mg plus ethambutol 50 mg/kg body weight under out-patient conditions after an initial inpatient phase of rifampicin 600 mg and ethambutol 25 mg/kg daily for 12 weeks. Patients were allocated at random to the regimens. Of 247 patients admitted to the study, 201 (81 per cent) completed 1 year's treatment as prescribed by the protocol, 46 (19 per cent) patients terminated their treatment prematurely before 1 year. After the daily phase of 12 weeks' treatment, 82 per cent were negative on smear and 85 per cent on culture; in the continuation intermittent phase, 98 per cent of patients in the once-weekly (E1R1) regimen were negative on culture at 28 weeks and 98 per cent in the twice-weekly (E2R2) regimen. The corresponding proportions at 52 weeks were 97 per cent and 97 per cent. At 12 months, 96 per cent of 101 ER/E1R1 and 96 per cent of 100 ER/E2R2 patients who completed 1 years' treatment were culture-negative."}
{"id": "PMID:1096389", "title": "A trial to investigate reactions and responses to BCG vaccines of different strengths prepared from the Copenhagen 1331 strain.", "content": "Seven hundred and ninety-three tuberculin-negative school-children were vaccinated with BCG vaccine prepared from either the 1077 substrain, at 0-3 mg/ml moist weight, or the 1331 substrain at 0-15 and 0-3 mg/ml moist weight. Local vaccination reactions and Mantoux tuberculin conversion were measured. There were no differences between the 1077 vaccine and the lower strength 1331 vaccine. The more potent 1331 product, however, produced marginally larger vaccination lesions and a slightly increased tuberculin allergy. The small increase in size of the vaccination lesions was considered acceptable and vaccine of this type would be satisfactory for use in the United Kingdom.", "contents": "A trial to investigate reactions and responses to BCG vaccines of different strengths prepared from the Copenhagen 1331 strain. Seven hundred and ninety-three tuberculin-negative school-children were vaccinated with BCG vaccine prepared from either the 1077 substrain, at 0-3 mg/ml moist weight, or the 1331 substrain at 0-15 and 0-3 mg/ml moist weight. Local vaccination reactions and Mantoux tuberculin conversion were measured. There were no differences between the 1077 vaccine and the lower strength 1331 vaccine. The more potent 1331 product, however, produced marginally larger vaccination lesions and a slightly increased tuberculin allergy. The small increase in size of the vaccination lesions was considered acceptable and vaccine of this type would be satisfactory for use in the United Kingdom."}
{"id": "PMID:1096396", "title": "Propantheline bromide in massive upper gastrointestinal haemorrhage.", "content": "The effectiveness of the anticholinergic, propantheline bromide, administered parenterally in patients with upper gastro-intestinal bleeding has been investigated in a double-blind study. The basic material consisted of 99 patients. No differences were found between the group receiving propantheline bromide and the group receiving placebo in regard to clinical factors such as duration of intensive care, total hospital stay and surgical frequency. However, 21 patients under 50 years of age showed a significantly lower blood transfusion requirement with the use of propantheline bromide.", "contents": "Propantheline bromide in massive upper gastrointestinal haemorrhage. The effectiveness of the anticholinergic, propantheline bromide, administered parenterally in patients with upper gastro-intestinal bleeding has been investigated in a double-blind study. The basic material consisted of 99 patients. No differences were found between the group receiving propantheline bromide and the group receiving placebo in regard to clinical factors such as duration of intensive care, total hospital stay and surgical frequency. However, 21 patients under 50 years of age showed a significantly lower blood transfusion requirement with the use of propantheline bromide."}
{"id": "PMID:1096397", "title": "Cardiac output determinations with ear piece densitometry.", "content": "The results of cardiac output determinations by a dye dilution technique were compared using (a) a dichromatic earpiece which was calibrated as a flow-through cuvette, but also permitted automatic computing by virtue of a pressure capsule, and (b) an ordinary flow-through densitometer. Eleven subjects, some with cardio-pulmonary disease, were investigated. Cardiac outputs were systematically overestimated when automatically computed. The results obtained by manual calculation with the ear-piece corresponded more nearly with those derived from the flow-through cuvette, but still with a deviation from the identity line and with a residual standard deviation of 0.8 l/min. Double determinations had a residual standard deviation of 0.7 l/min. Despite its ease of handling, an earpiece densitometer seems to be too unreliable to be suitable for routine use.", "contents": "Cardiac output determinations with ear piece densitometry. The results of cardiac output determinations by a dye dilution technique were compared using (a) a dichromatic earpiece which was calibrated as a flow-through cuvette, but also permitted automatic computing by virtue of a pressure capsule, and (b) an ordinary flow-through densitometer. Eleven subjects, some with cardio-pulmonary disease, were investigated. Cardiac outputs were systematically overestimated when automatically computed. The results obtained by manual calculation with the ear-piece corresponded more nearly with those derived from the flow-through cuvette, but still with a deviation from the identity line and with a residual standard deviation of 0.8 l/min. Double determinations had a residual standard deviation of 0.7 l/min. Despite its ease of handling, an earpiece densitometer seems to be too unreliable to be suitable for routine use."}
{"id": "PMID:1096398", "title": "Plasma erythropoietin activity before and after renal homotransplantation in humans.", "content": "Plasma erythropoietin levels were determined by Keighley's method to study the changes in erythropoiesis before and after human renal homotransplantation. Results. Erythropoietin titres in pre-transplant patients were low, while they returned to normal after successful renal transplantation. In acute rejection they were significantly high. After its reversal normal levels of erythropoietin were obtained in accordance with a normalization of the graft functions. Reticulocyte counts paralleled with erythropoietin values. Conclusion. High levels of plasma erythropoietin contribute to the diagnosis of acute rejection. Normalization of the plasma erythropoietin levels after the acute rejection could be regarded as an indication for good function of the graft. The grafted kidneys seem to function in producing erythropoietin.", "contents": "Plasma erythropoietin activity before and after renal homotransplantation in humans. Plasma erythropoietin levels were determined by Keighley's method to study the changes in erythropoiesis before and after human renal homotransplantation. Results. Erythropoietin titres in pre-transplant patients were low, while they returned to normal after successful renal transplantation. In acute rejection they were significantly high. After its reversal normal levels of erythropoietin were obtained in accordance with a normalization of the graft functions. Reticulocyte counts paralleled with erythropoietin values. Conclusion. High levels of plasma erythropoietin contribute to the diagnosis of acute rejection. Normalization of the plasma erythropoietin levels after the acute rejection could be regarded as an indication for good function of the graft. The grafted kidneys seem to function in producing erythropoietin."}
{"id": "PMID:1096400", "title": "Regional differences in patterns of urinary tract infections.", "content": "The identity of 693 pathogenic bacilli isolated from 2,175 urine specimens cultured during a two-year period in southeastern New Mexico is presented along with results of sensitivity testing by Kirby-Bauer technique.  The pattern of infections and sensitivity studies in hospitalized patients is compared with that of office patients and contrasted with results noted in other areas.", "contents": "Regional differences in patterns of urinary tract infections. The identity of 693 pathogenic bacilli isolated from 2,175 urine specimens cultured during a two-year period in southeastern New Mexico is presented along with results of sensitivity testing by Kirby-Bauer technique.  The pattern of infections and sensitivity studies in hospitalized patients is compared with that of office patients and contrasted with results noted in other areas."}
{"id": "PMID:1096401", "title": "Urinary tract infections in black female children.", "content": "It has been demonstrated in several studies that prepubertal black females have a lower incidence of urinary tract infection than do white females of the same age. Other racial differences in the natural history of urinary tract infection have been suggested, but thus far very few young black girls with urinary tract infection have been thoroughly studied. The records of 56 black female children with urinary tract infections were reviewed and the clinical, laboratory, and radiologic manifestations of their diseases documented. No attempt was made to compare data from blacks to that from whites since many studies composed primarily of white children are available in recent literature. This study is offered as baseline data on black female children to which future studies of black or white children may be compared.", "contents": "Urinary tract infections in black female children. It has been demonstrated in several studies that prepubertal black females have a lower incidence of urinary tract infection than do white females of the same age. Other racial differences in the natural history of urinary tract infection have been suggested, but thus far very few young black girls with urinary tract infection have been thoroughly studied. The records of 56 black female children with urinary tract infections were reviewed and the clinical, laboratory, and radiologic manifestations of their diseases documented. No attempt was made to compare data from blacks to that from whites since many studies composed primarily of white children are available in recent literature. This study is offered as baseline data on black female children to which future studies of black or white children may be compared."}
{"id": "PMID:1096402", "title": "Wallace method of ureteroileal anastomosis.", "content": "The Wallace technique for ureteroileal anastomosis was used in 28 consecutive patients requiring urinary diversion. Details of the surgical technique are reported. The method is found to be appropriate for children and adults, for primary ureteroileal anastomosis, or for revision of previous ureteroileal anastomosis done by other techniques, and for patients with one or two ureters. Its continued use appears to be indicated.", "contents": "Wallace method of ureteroileal anastomosis. The Wallace technique for ureteroileal anastomosis was used in 28 consecutive patients requiring urinary diversion. Details of the surgical technique are reported. The method is found to be appropriate for children and adults, for primary ureteroileal anastomosis, or for revision of previous ureteroileal anastomosis done by other techniques, and for patients with one or two ureters. Its continued use appears to be indicated."}
{"id": "PMID:1096403", "title": "Transplantation in the hemolytic-uremic syndrome.", "content": "A case history of a fifty-seven-year-old white woman with hemolytic-uremic syndrome who was successfully transplanted with a cadaver homograft is reported. A review of the pertinent literature regarding the pathophysiology of the syndrome and the experience to date with transplantation is presented.", "contents": "Transplantation in the hemolytic-uremic syndrome. A case history of a fifty-seven-year-old white woman with hemolytic-uremic syndrome who was successfully transplanted with a cadaver homograft is reported. A review of the pertinent literature regarding the pathophysiology of the syndrome and the experience to date with transplantation is presented."}
{"id": "PMID:1096404", "title": "Reversal of hypercoagulable state by bilateral nephrectomy.", "content": "The hypercoagulable state associated with select cases of glomerulonephritis is difficult to diagnose by standard laboratory techniques but is clinically manifested by recurrent shunt and fistula thrombosis which may be refractory to anticoagulant therapy. Bilateral nephrectomy reversed this hypercoagulable state. After renal allograft, however, the patient experienced hyperacute rejection, and transplant nephrectomy was required to cure the recurrent hypercoagulable state. The mechanism of this previously unreported phenomenon and its implications with regard to renal transplantation are discussed.", "contents": "Reversal of hypercoagulable state by bilateral nephrectomy. The hypercoagulable state associated with select cases of glomerulonephritis is difficult to diagnose by standard laboratory techniques but is clinically manifested by recurrent shunt and fistula thrombosis which may be refractory to anticoagulant therapy. Bilateral nephrectomy reversed this hypercoagulable state. After renal allograft, however, the patient experienced hyperacute rejection, and transplant nephrectomy was required to cure the recurrent hypercoagulable state. The mechanism of this previously unreported phenomenon and its implications with regard to renal transplantation are discussed."}
{"id": "PMID:1096406", "title": "Cadaver donor nephrectomy.", "content": "Optimal preservation of renal and ureteral structure and function is attempted by maintenance of renal circulation during heart-beating cadaver nephrectomy. Modifications of Ackermann's en bloc procedure for removal of cadaver kidneys are suggested to adapt this procedure to the heart-beating cadaver situation.", "contents": "Cadaver donor nephrectomy. Optimal preservation of renal and ureteral structure and function is attempted by maintenance of renal circulation during heart-beating cadaver nephrectomy. Modifications of Ackermann's en bloc procedure for removal of cadaver kidneys are suggested to adapt this procedure to the heart-beating cadaver situation."}
{"id": "PMID:1096408", "title": "Polycythemia following renal transplantation.", "content": "Among 60 renal allograft recipients, 3 patients were found to be polycythemic  with hematocrits greater than 55 and were studied extensively with various hematologic and radiographic procedures. None of the patients showed evidence of erythropoietin-secreting malignancies, renal cysts, hydronephrosis, or hypoxemia. All had elevated erythropoietin levels, and 1 showed evidence of platelet trapping in his transplanted kidney, suggesting that the chronic polycythemia may be related to transplant rejection.", "contents": "Polycythemia following renal transplantation. Among 60 renal allograft recipients, 3 patients were found to be polycythemic  with hematocrits greater than 55 and were studied extensively with various hematologic and radiographic procedures. None of the patients showed evidence of erythropoietin-secreting malignancies, renal cysts, hydronephrosis, or hypoxemia. All had elevated erythropoietin levels, and 1 showed evidence of platelet trapping in his transplanted kidney, suggesting that the chronic polycythemia may be related to transplant rejection."}
{"id": "PMID:1096409", "title": "Experimental evaluation of bladder closure techniques.", "content": "Two methods of bladder closure have been evaluated in dogs. A conventional two-layer closure including the mucosa and a single layer running horizontal mattress extramucosal closure have been compared. Observations including adequacy of healing, strength of scar, degree of inflammatory reaction, dna degree of bladder adhesions have been made. The extramucosal running horizontal mattress closure appears to approach the ideal bladder closure.", "contents": "Experimental evaluation of bladder closure techniques. Two methods of bladder closure have been evaluated in dogs. A conventional two-layer closure including the mucosa and a single layer running horizontal mattress extramucosal closure have been compared. Observations including adequacy of healing, strength of scar, degree of inflammatory reaction, dna degree of bladder adhesions have been made. The extramucosal running horizontal mattress closure appears to approach the ideal bladder closure."}
{"id": "PMID:1096410", "title": "Acute renal failure in sickle cell trait.", "content": "Acute renal failure and a syndrome characteristic of acute renal vein thrombosis developed in a patient with sickle trait and gram-negative bacteremia. It is suggested that intrarenal vaso-occlusion by sickled erythrocytes produced signs and symptoms typical of renal vein thrombosis and contributed to the severity of the acute renal failure.", "contents": "Acute renal failure in sickle cell trait. Acute renal failure and a syndrome characteristic of acute renal vein thrombosis developed in a patient with sickle trait and gram-negative bacteremia. It is suggested that intrarenal vaso-occlusion by sickled erythrocytes produced signs and symptoms typical of renal vein thrombosis and contributed to the severity of the acute renal failure."}
{"id": "PMID:1096411", "title": "Traveling bullets in genitourinary tract.", "content": "A bullet lodged in the renal parenchyma, then migrated into the collecting system over a two-month period causing acute renal colic and necessitating removal by pyelotomy. A review of the literature discloses only 8 cases of projectiles migrating in the urinary tract, 5 involving metallic fragments and 3 involving bullets.", "contents": "Traveling bullets in genitourinary tract. A bullet lodged in the renal parenchyma, then migrated into the collecting system over a two-month period causing acute renal colic and necessitating removal by pyelotomy. A review of the literature discloses only 8 cases of projectiles migrating in the urinary tract, 5 involving metallic fragments and 3 involving bullets."}
{"id": "PMID:1096417", "title": "Prototype for an implantable micropump powdered by piezoelectric disk benders.", "content": "A prototype for an implantable mul pump to be used in an artificial pancreas has been constructed, in which pressure is developed by a bellows formed from 2 apposed piezoelectric disk benders connected to the high impedance secondary winding of aminiature transformer. Active valving, properly phased, is achieved by a miniature solenoid valve connected in series with the transformer primary winding and a rectangular wave generator.", "contents": "Prototype for an implantable micropump powdered by piezoelectric disk benders. A prototype for an implantable mul pump to be used in an artificial pancreas has been constructed, in which pressure is developed by a bellows formed from 2 apposed piezoelectric disk benders connected to the high impedance secondary winding of aminiature transformer. Active valving, properly phased, is achieved by a miniature solenoid valve connected in series with the transformer primary winding and a rectangular wave generator."}
{"id": "PMID:1096420", "title": "Continuous single pass perfusion of the isolated kidney.", "content": "The use of continuous single pass perfusion to study the metabolism of isolated kidneys reveals that active metabolic processes continue in the hypothermically preserved kidney. These preliminary experiments described demonstrated a rapid uptake of substrates from the perfusion media in the first 24 hrs of storage, followed by a release of organic acids in the subsequent 24 to 72 hrs. Urea was found to be easily eliminated from the kidney. This technique may be useful for studying events related to loss of renal function by pinpointing specific residual biochemical activity and lesions that occur during prolonged preservations. An eventual correction or prevention of such lesions might be feasible in order to improve preservation techniques.", "contents": "Continuous single pass perfusion of the isolated kidney. The use of continuous single pass perfusion to study the metabolism of isolated kidneys reveals that active metabolic processes continue in the hypothermically preserved kidney. These preliminary experiments described demonstrated a rapid uptake of substrates from the perfusion media in the first 24 hrs of storage, followed by a release of organic acids in the subsequent 24 to 72 hrs. Urea was found to be easily eliminated from the kidney. This technique may be useful for studying events related to loss of renal function by pinpointing specific residual biochemical activity and lesions that occur during prolonged preservations. An eventual correction or prevention of such lesions might be feasible in order to improve preservation techniques."}
{"id": "PMID:1096421", "title": "Improved renal allograft survival using the mixed lymphocyte culture for selection of nonidentical living related donors.", "content": "Our results concur with earlier published work, by other groups, showing that LRD-recipient pairs with low MLC stimulation usually have better and more prolonged graft success than do those with higher stimulation. Specific HL-A compatibilities or incompatibilities did not seem to affect these results, nor did the presence of an increased number of common loci, short of increasing the apparent chromosome compatibility. The presence of pre-transplant cytotoxic antibodies, in patients with a high MI, however, may unfavorably affect the LRD transplant. The overall results of our LRD transplant experience is shown in Figure 1, and superimposed upon Figure 2, is the current extrapolation of data showing MLC stimulation and haplotype success. Thus, it appears that graft survival may be improved and more closely approach the levels seen in a full-house, diplotype match, by using the MLC results in considering patients for transplantation. Not all patients with a high MLC, however, (see table) reject their grafts and it is impossible to predict pre-transplant who will develop specific allograft enhancement. Before the MI becomes a specific criteria for transplant selection, additional studies of patient stimulation in MLC should be done. Suppression of stimulation by donor cells in autologous serum, as compared to the response to unrelated controls, might provide pre-transplant clues to the presence of enhancing factors. Such studies could provide an index that would be more meaningful than the MI in AB sera alone. Since overall results from both our series and from the Transplant Registry continue to indicate better long term graft survival for LRD than for cadaver transplants, and since the evidence suggests that a successful transplant offers a patient a better quality of life, as well as decreased morbidity and mortality compared to concomitant time spent on hemodialysis, continued LRD transplants with high MI is warranted in some circumstances with the patient's understanding of the overall outcome and backgrouns. Until in vitro tests can be more predictive of individual rather than statistical graft success, the patient with an LRD and a high MI should be given a complete explanation of the probabilities for success or failure, and he and the donor allowed to make an informed decision. The following are guidelines that we proposed for the selection of LRD transplants: 1. Availability and willingness. 2. Medical and psycho-social clearance. 3. ABO compatibility. 4. Negative direct crossmatch. 5. HL-A diplotype or haplotype. 6. MI less than 10 if possible, with or without HL-A antibodies. 7. MI greater than 10 with no HL-A antibodies, if possible. 8. MI greater than 10, with HL-A antibodies, if both donor and recipient clearly understand the enhanced chance for failure.", "contents": "Improved renal allograft survival using the mixed lymphocyte culture for selection of nonidentical living related donors. Our results concur with earlier published work, by other groups, showing that LRD-recipient pairs with low MLC stimulation usually have better and more prolonged graft success than do those with higher stimulation. Specific HL-A compatibilities or incompatibilities did not seem to affect these results, nor did the presence of an increased number of common loci, short of increasing the apparent chromosome compatibility. The presence of pre-transplant cytotoxic antibodies, in patients with a high MI, however, may unfavorably affect the LRD transplant. The overall results of our LRD transplant experience is shown in Figure 1, and superimposed upon Figure 2, is the current extrapolation of data showing MLC stimulation and haplotype success. Thus, it appears that graft survival may be improved and more closely approach the levels seen in a full-house, diplotype match, by using the MLC results in considering patients for transplantation. Not all patients with a high MLC, however, (see table) reject their grafts and it is impossible to predict pre-transplant who will develop specific allograft enhancement. Before the MI becomes a specific criteria for transplant selection, additional studies of patient stimulation in MLC should be done. Suppression of stimulation by donor cells in autologous serum, as compared to the response to unrelated controls, might provide pre-transplant clues to the presence of enhancing factors. Such studies could provide an index that would be more meaningful than the MI in AB sera alone. Since overall results from both our series and from the Transplant Registry continue to indicate better long term graft survival for LRD than for cadaver transplants, and since the evidence suggests that a successful transplant offers a patient a better quality of life, as well as decreased morbidity and mortality compared to concomitant time spent on hemodialysis, continued LRD transplants with high MI is warranted in some circumstances with the patient's understanding of the overall outcome and backgrouns. Until in vitro tests can be more predictive of individual rather than statistical graft success, the patient with an LRD and a high MI should be given a complete explanation of the probabilities for success or failure, and he and the donor allowed to make an informed decision. The following are guidelines that we proposed for the selection of LRD transplants: 1. Availability and willingness. 2. Medical and psycho-social clearance. 3. ABO compatibility. 4. Negative direct crossmatch. 5. HL-A diplotype or haplotype. 6. MI less than 10 if possible, with or without HL-A antibodies. 7. MI greater than 10 with no HL-A antibodies, if possible. 8. MI greater than 10, with HL-A antibodies, if both donor and recipient clearly understand the enhanced chance for failure."}
{"id": "PMID:1096441", "title": "The use of rapeseed meal as a protein supplement in poultry and pig diets.", "content": "Rapeseed meal would be used in much larger amounts in poultry and pig diets if its use was related solely to cost and nutritive value. In certain circumstances there are undesirable effects that severely lmiit its use. Most of the rapeseed meal available at present in the UK is strongly goitrogenic when fed to poultry or pigs, but it is not certain if this is necessarily harmful, nor if this is related to other effects that occur in particular situations and are clearly undesirable. From current data, diets in which rapeseed meal can be included most satisfactorily are those for broilers, growing pullets and finishing pigs. Plant breeders are actively selecting types of rape that have low or zero thioglucoside content and it is hoped that these new varieties will cause none of the ill-effects described in this review.", "contents": "The use of rapeseed meal as a protein supplement in poultry and pig diets. Rapeseed meal would be used in much larger amounts in poultry and pig diets if its use was related solely to cost and nutritive value. In certain circumstances there are undesirable effects that severely lmiit its use. Most of the rapeseed meal available at present in the UK is strongly goitrogenic when fed to poultry or pigs, but it is not certain if this is necessarily harmful, nor if this is related to other effects that occur in particular situations and are clearly undesirable. From current data, diets in which rapeseed meal can be included most satisfactorily are those for broilers, growing pullets and finishing pigs. Plant breeders are actively selecting types of rape that have low or zero thioglucoside content and it is hoped that these new varieties will cause none of the ill-effects described in this review."}
{"id": "PMID:1096446", "title": "Oral immunisation and its significance in the prophylactic control of enteritis in the preruminant calf.", "content": "Oral immunisation using a feed-incorporated vaccine comprising heat inactivated Escherichia coli and Salmonellae antigens was examined in feeding trials with calves. The studies were conducted with batches of calves purchased at intervals throughout one year and reared in an environment created by continuous occupancy. Improved health was registered in terms of a significant reduction in the incidence and duration of diarrhoea (P less than 0.01) and in the need for treatment with medicament or antibiotics (P less than 0.01). Orally immunised calves showed a significantly better weight gain (1.44 kg, P less than 0.05) than the controls during the period 0 to 5 weeks. This gain was mainly obtained during the first three weeks when the antigen fed calves showed significantly improved faeces scores and a significantly better appetite for concentrates (P less than 0.05).", "contents": "Oral immunisation and its significance in the prophylactic control of enteritis in the preruminant calf. Oral immunisation using a feed-incorporated vaccine comprising heat inactivated Escherichia coli and Salmonellae antigens was examined in feeding trials with calves. The studies were conducted with batches of calves purchased at intervals throughout one year and reared in an environment created by continuous occupancy. Improved health was registered in terms of a significant reduction in the incidence and duration of diarrhoea (P less than 0.01) and in the need for treatment with medicament or antibiotics (P less than 0.01). Orally immunised calves showed a significantly better weight gain (1.44 kg, P less than 0.05) than the controls during the period 0 to 5 weeks. This gain was mainly obtained during the first three weeks when the antigen fed calves showed significantly improved faeces scores and a significantly better appetite for concentrates (P less than 0.05)."}
{"id": "PMID:1096470", "title": "A new solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody.", "content": "A new competitive solid phase radioimmunoassay (CLB-RIA) has been developed for the detection of HBAg and HBAb in human serum and plasma. In the assay, sheep antibodies to HBAg, covalently linked to an insoluble carrier, highly purified 125-I labelled HBAg and the serum or plasma sample are incubated for 20 h at room temperature. After incubation, the bound and the free fraction of the tracer are separated by centrifugation. Both the presence of HBAg and HBAb, result in a decrease of the amount of bound tracer, when compared with a negative control serum. Differentiation between HBAg and HBAb requires the use of another type of radioimmunoassay. For this purpose a sandwich solid phase radioimmunoassay, for the detection of HBAb only, has been developed (CLB-AURIA). In this, assay-purified HBAg is covalently linked to an insoluble carrier. Using a mixture of both immunosorbents (insolubilized HBAg and HBAb), it is possible to detect and to  distinguish HBAg and HBAb in one single solid phase radioimmunoassay (CLB-MIRIA). The influence of three parameters on the CLB-RIA, the incubation time, the amount of tracer and the effect of Tween-20 has been studied. The sensitivity of the described solid phase CLB-RIA for the detection of HBAg is comparable to that of other radioimmunoassays reported in literature; its specificity is very high.", "contents": "A new solid phase radioimmunoassay (CLB-RIA) for the detection of hepatitis-B antigen and antibody. A new competitive solid phase radioimmunoassay (CLB-RIA) has been developed for the detection of HBAg and HBAb in human serum and plasma. In the assay, sheep antibodies to HBAg, covalently linked to an insoluble carrier, highly purified 125-I labelled HBAg and the serum or plasma sample are incubated for 20 h at room temperature. After incubation, the bound and the free fraction of the tracer are separated by centrifugation. Both the presence of HBAg and HBAb, result in a decrease of the amount of bound tracer, when compared with a negative control serum. Differentiation between HBAg and HBAb requires the use of another type of radioimmunoassay. For this purpose a sandwich solid phase radioimmunoassay, for the detection of HBAb only, has been developed (CLB-AURIA). In this, assay-purified HBAg is covalently linked to an insoluble carrier. Using a mixture of both immunosorbents (insolubilized HBAg and HBAb), it is possible to detect and to  distinguish HBAg and HBAb in one single solid phase radioimmunoassay (CLB-MIRIA). The influence of three parameters on the CLB-RIA, the incubation time, the amount of tracer and the effect of Tween-20 has been studied. The sensitivity of the described solid phase CLB-RIA for the detection of HBAg is comparable to that of other radioimmunoassays reported in literature; its specificity is very high."}
{"id": "PMID:1096471", "title": "The effective oncotic pressure of dextran.", "content": "Measurement of plasma volume before and after the acute infusion of commercial dextran revealed that all brands tested were hyperoncotic by a factor of approximately 2. By mixing 6% dextran with 0.9% saline in 1:1 proportion a 3% dextran solution was obtained which was nearly isoncotic in our dogs. The most likely explanation for the discrepancy is that the 6% solution contains a high proportion of molecules of lower molecular weight resulting in a greater oncotic pressure than predicted on the basis of the average molecular weight. In addition, this leads to a rapid loss of this solute from the blood. Clinicians and researchers should be aware of the possibility that the dextran preparation they are using is not truly isoncotic.", "contents": "The effective oncotic pressure of dextran. Measurement of plasma volume before and after the acute infusion of commercial dextran revealed that all brands tested were hyperoncotic by a factor of approximately 2. By mixing 6% dextran with 0.9% saline in 1:1 proportion a 3% dextran solution was obtained which was nearly isoncotic in our dogs. The most likely explanation for the discrepancy is that the 6% solution contains a high proportion of molecules of lower molecular weight resulting in a greater oncotic pressure than predicted on the basis of the average molecular weight. In addition, this leads to a rapid loss of this solute from the blood. Clinicians and researchers should be aware of the possibility that the dextran preparation they are using is not truly isoncotic."}
{"id": "PMID:1096474", "title": "Erythroblastemia.", "content": "The significance of erythroblastemia must be considered in the context of the clinical setting in which it is found. Interpretation should take into account the number and spectrum of maturity of the nucleated red cells; the presence or absence of reticulocytosis and other red cell abnormalities (for example, poikilocytosis); the presence or absence of anemia; the presence or absence of circulating immature granulocytes or bizarre platelets and the presence or absence of the spleen. Circulating nucleated red cells indicate intravascular hemopoiesis or disruption of marrow structure or the inability of the bone marrow's screen mechanism to prevent their passage into circulation. In the latter situations, it usually indicates an unfavorable prognosis.", "contents": "Erythroblastemia. The significance of erythroblastemia must be considered in the context of the clinical setting in which it is found. Interpretation should take into account the number and spectrum of maturity of the nucleated red cells; the presence or absence of reticulocytosis and other red cell abnormalities (for example, poikilocytosis); the presence or absence of anemia; the presence or absence of circulating immature granulocytes or bizarre platelets and the presence or absence of the spleen. Circulating nucleated red cells indicate intravascular hemopoiesis or disruption of marrow structure or the inability of the bone marrow's screen mechanism to prevent their passage into circulation. In the latter situations, it usually indicates an unfavorable prognosis."}
{"id": "PMID:1096482", "title": "An electron microscopic and cytochemical study of the cell coat of Trypanosoma cruzi in tissue cultures.", "content": "Using the cytochemical method of Thiery, a polysaccharide surface coat was demonstrated with the electron microscope in the various developmental stages of Trypanosoma cruzi maintained in tissue cultures. The cell coat was observed on the whole surface membrane system of T. cruzi. A positive reaction, similar to that observed in the cell membrane, was also obtained in the membranes of some vacuoles in the cytoplasm of the parasites. No granules were found in the parasites. No granules were found in the parasite which could represent reserve polysaccharides. The possible role of the cell coat in such phenomena as adhesion, agglutination, phago- and pinocytosis and antigenicity is discussed.", "contents": "An electron microscopic and cytochemical study of the cell coat of Trypanosoma cruzi in tissue cultures. Using the cytochemical method of Thiery, a polysaccharide surface coat was demonstrated with the electron microscope in the various developmental stages of Trypanosoma cruzi maintained in tissue cultures. The cell coat was observed on the whole surface membrane system of T. cruzi. A positive reaction, similar to that observed in the cell membrane, was also obtained in the membranes of some vacuoles in the cytoplasm of the parasites. No granules were found in the parasites. No granules were found in the parasite which could represent reserve polysaccharides. The possible role of the cell coat in such phenomena as adhesion, agglutination, phago- and pinocytosis and antigenicity is discussed."}
{"id": "PMID:1096483", "title": "Immunoprecipitation and class-specific immunofluorescence titration of human serum antibodies to Schistosoma mansoni antigens.", "content": "Twenty sera of individuals with Schistosoma mansoni infections were examined by means of immunoelectrophoresis, Indirect Flourescent Antibody (IFA) technique and the Defined Antigen Substrate Spheres (DASS) system. Immunoprecipitins against S. mansoni antigen and against antigen of the intermediate host, Biomphalaria glabrata, were demonstrated. The presence of specific anti-S. mansoni IgA, IgM, IgG and IgE antibodies were demonstrated. The presence of specific anti-S. MANSONI IgA, IgM, IgG and IgE antibodies was identified with the IFA-technique on frozen sections of adult parasites. The DASS-system proved to be more sensitive than the IFA-technique.", "contents": "Immunoprecipitation and class-specific immunofluorescence titration of human serum antibodies to Schistosoma mansoni antigens. Twenty sera of individuals with Schistosoma mansoni infections were examined by means of immunoelectrophoresis, Indirect Flourescent Antibody (IFA) technique and the Defined Antigen Substrate Spheres (DASS) system. Immunoprecipitins against S. mansoni antigen and against antigen of the intermediate host, Biomphalaria glabrata, were demonstrated. The presence of specific anti-S. mansoni IgA, IgM, IgG and IgE antibodies were demonstrated. The presence of specific anti-S. MANSONI IgA, IgM, IgG and IgE antibodies was identified with the IFA-technique on frozen sections of adult parasites. The DASS-system proved to be more sensitive than the IFA-technique."}
{"id": "PMID:1096503", "title": "[Phage typing and biotyping of salmonella typhi-murium (author's transl)].", "content": "1998 strains of Salmonella typhi-murium from two different regions of West-Germany were investigated according to their biotype and phagetype. 132 strains were isolated from sewage, 320 strains from animals and the others from human beings. 60 different phagetypes were found. The phagetype 49 occurred most often (11,9% resp. 10,3%). A correlation between biotype and phagetype could not be observed. Nearly every phagetype could be divided into several biotypes. However, only few biotypes prevaled. These comprised the biotypes 1, 10 and 17. The examination of the splitting of trehalose was useful in the biochemical differentiation. Concerning strains of animal origin it was remarkable that most strains from cattle resp. calves belonged to the phagetype 49. Among 145 strains from pigeons 95,9% could be classified as phagetype 2. With exception of 3 strains all the other strains from pigeons did not possess the O-antigen 5. The majority of the strains belonged to the biotype 8. All these strains could not split maltose.", "contents": "[Phage typing and biotyping of salmonella typhi-murium (author's transl)]. 1998 strains of Salmonella typhi-murium from two different regions of West-Germany were investigated according to their biotype and phagetype. 132 strains were isolated from sewage, 320 strains from animals and the others from human beings. 60 different phagetypes were found. The phagetype 49 occurred most often (11,9% resp. 10,3%). A correlation between biotype and phagetype could not be observed. Nearly every phagetype could be divided into several biotypes. However, only few biotypes prevaled. These comprised the biotypes 1, 10 and 17. The examination of the splitting of trehalose was useful in the biochemical differentiation. Concerning strains of animal origin it was remarkable that most strains from cattle resp. calves belonged to the phagetype 49. Among 145 strains from pigeons 95,9% could be classified as phagetype 2. With exception of 3 strains all the other strains from pigeons did not possess the O-antigen 5. The majority of the strains belonged to the biotype 8. All these strains could not split maltose."}
{"id": "PMID:1096504", "title": "Membrane damage and incorporation of Escherichia coli components into Bdellovibrio bacteriovorus.", "content": "Cytoplasmic membrane of E. coli is degraded within 20 min following infection with Bdellovibrio. 50% of cellular 42-K is lost during the first 10 min. The cytoplasmic membrane, 20 min after infection, centrifugated on a sucrose gradient produces a wide band containing the main enzyme activities (succinic dehydrogenase and lactic dehydrogenase) bound to the membrane. The incorporation into Bdellovibrio of labelled host cell constituents during intracellular growth has been studied at successive intervals during the development cycle in diluted nutrient broth (about 3 hrs). The cells were broken in a Sorvall-Ribi cell fractionator and the Bdellovibrios separated by centrifugation on sucrose gradient. Polysaccharides, proteins and lipids of Bdellovibrio derive from the utilization of components of the host cells and not from the utilization of the components present in the medium. The incorporation of precursors into polysaccharides and proteins shows the same exponential pattern.", "contents": "Membrane damage and incorporation of Escherichia coli components into Bdellovibrio bacteriovorus. Cytoplasmic membrane of E. coli is degraded within 20 min following infection with Bdellovibrio. 50% of cellular 42-K is lost during the first 10 min. The cytoplasmic membrane, 20 min after infection, centrifugated on a sucrose gradient produces a wide band containing the main enzyme activities (succinic dehydrogenase and lactic dehydrogenase) bound to the membrane. The incorporation into Bdellovibrio of labelled host cell constituents during intracellular growth has been studied at successive intervals during the development cycle in diluted nutrient broth (about 3 hrs). The cells were broken in a Sorvall-Ribi cell fractionator and the Bdellovibrios separated by centrifugation on sucrose gradient. Polysaccharides, proteins and lipids of Bdellovibrio derive from the utilization of components of the host cells and not from the utilization of the components present in the medium. The incorporation of precursors into polysaccharides and proteins shows the same exponential pattern."}
{"id": "PMID:1096505", "title": "Classification of coagulase-negative staphylococci of human origin.", "content": "A total of 191 coagulase-negative staphylococci isolated from man were identified as Staphylococcus epidermidis on the basis of their biochemical properties. When classified according to the classification scheme suggested by BAIRD-PARKER (1972), 144 of the strains were found to belong to biotype 1, one strain was biotype 2, and four were biotype 3; the remaining 42 strains could not be classified within any of the 4 biotypes of this scheme. Using the classification scheme suggested by PELZER et al. (1973), 158 strains were found to belong to subgroup IIb, one was subgroup IIIb, four were subgroup IV, and 28 were subgroup V, with no strains remaining unclassified. The biochemical properties of the strains were not related to the site of isolation except that urease was produced by only 15 per cent of strains isolated from pus but by 81-92 per cent of strains isolated from a variety of other sources. The majority of the strains (64 per cent) were resistant to penicillin, while 72 per cent were sensitive to streptomycin.", "contents": "Classification of coagulase-negative staphylococci of human origin. A total of 191 coagulase-negative staphylococci isolated from man were identified as Staphylococcus epidermidis on the basis of their biochemical properties. When classified according to the classification scheme suggested by BAIRD-PARKER (1972), 144 of the strains were found to belong to biotype 1, one strain was biotype 2, and four were biotype 3; the remaining 42 strains could not be classified within any of the 4 biotypes of this scheme. Using the classification scheme suggested by PELZER et al. (1973), 158 strains were found to belong to subgroup IIb, one was subgroup IIIb, four were subgroup IV, and 28 were subgroup V, with no strains remaining unclassified. The biochemical properties of the strains were not related to the site of isolation except that urease was produced by only 15 per cent of strains isolated from pus but by 81-92 per cent of strains isolated from a variety of other sources. The majority of the strains (64 per cent) were resistant to penicillin, while 72 per cent were sensitive to streptomycin."}
{"id": "PMID:1096506", "title": "Frequency distribution of S. typhi-murium phage types in various countries.", "content": "The phage type and antibiogram of 5199 S. typhi murium strains from 13 countries and of 4949 strains from The Netherlands was determined. For a number of countries it could be established that types predominating in pigs or calves predominate also in humans in that country. However, the predominant phage types were not the same in the various countries. Type 505 predominated in The Netherlands and, to a lesser degree, also in Belgium and the German Federal Republic. Type 131 predominated in Poland and was also frequently found in the German Democratic Republic. In the German Democratic Republic, type 450 was the most frequently found type. Phage patterns not accepted as a type and occurring one country only, were found to predominate in Italy, Hungary, Israel and Sweden. The majority of antibiotic-resistant strains belonged to a limited number of phage types only.", "contents": "Frequency distribution of S. typhi-murium phage types in various countries. The phage type and antibiogram of 5199 S. typhi murium strains from 13 countries and of 4949 strains from The Netherlands was determined. For a number of countries it could be established that types predominating in pigs or calves predominate also in humans in that country. However, the predominant phage types were not the same in the various countries. Type 505 predominated in The Netherlands and, to a lesser degree, also in Belgium and the German Federal Republic. Type 131 predominated in Poland and was also frequently found in the German Democratic Republic. In the German Democratic Republic, type 450 was the most frequently found type. Phage patterns not accepted as a type and occurring one country only, were found to predominate in Italy, Hungary, Israel and Sweden. The majority of antibiotic-resistant strains belonged to a limited number of phage types only."}
{"id": "PMID:1096507", "title": "Studies on the energetic metabolism of Shigella flexneri times Escherichia coli hybrids devoid of penetration ability I. Glycolysis.", "content": "The aerobic and anaerobic (in presence of KCN) glycolysis is studied of avirulent hybrids of Shigella flexneri according to the keratoconjunctivitis test of Sereny with genetic characteristics lac-+kcp-minus devoid of penetration ability. It is established that lac-+kcp-minus hybrids which have lost their ability to penetrate the cells due to replacement of KCP-gene of the chromosome of donor strain E. coli K-12 P4 times, are characterized with a steep decrease of their glycolitic activity in both aerobic and anaerobic conditions. The data shows that the loss of penetrate ability of the Shigella flexneri hybrids correlates with the decrease of their glycolitic ability.", "contents": "Studies on the energetic metabolism of Shigella flexneri times Escherichia coli hybrids devoid of penetration ability I. Glycolysis. The aerobic and anaerobic (in presence of KCN) glycolysis is studied of avirulent hybrids of Shigella flexneri according to the keratoconjunctivitis test of Sereny with genetic characteristics lac-+kcp-minus devoid of penetration ability. It is established that lac-+kcp-minus hybrids which have lost their ability to penetrate the cells due to replacement of KCP-gene of the chromosome of donor strain E. coli K-12 P4 times, are characterized with a steep decrease of their glycolitic activity in both aerobic and anaerobic conditions. The data shows that the loss of penetrate ability of the Shigella flexneri hybrids correlates with the decrease of their glycolitic ability."}
{"id": "PMID:1096508", "title": "[The morphology of a nocardia asteroides-bacteriophage (author's transl)].", "content": "The morphology of a new bacteriophage isolated from Nocardia asteroides has been investigated by negative staining. The percent results suggest a model which is valid for many phages. The head of the bacteriophage has the shape of an icosaeder with a diameter of about 420 AU. It probably contains a spherical core which is surrounded by a capsid of 15 AU thickness. A flexible non-contractible tail is connected by a collar to one of the head's edges. Its length is about 1400 AU and its diameter 60 AU. The tail shows a cross striation with a periodicity of 40 AU and a channel. At the distal side it passes over into a conical end structure. Conforming this morphological criteria the new bacteriophage belongs to the group B of Bradley.", "contents": "[The morphology of a nocardia asteroides-bacteriophage (author's transl)]. The morphology of a new bacteriophage isolated from Nocardia asteroides has been investigated by negative staining. The percent results suggest a model which is valid for many phages. The head of the bacteriophage has the shape of an icosaeder with a diameter of about 420 AU. It probably contains a spherical core which is surrounded by a capsid of 15 AU thickness. A flexible non-contractible tail is connected by a collar to one of the head's edges. Its length is about 1400 AU and its diameter 60 AU. The tail shows a cross striation with a periodicity of 40 AU and a channel. At the distal side it passes over into a conical end structure. Conforming this morphological criteria the new bacteriophage belongs to the group B of Bradley."}
{"id": "PMID:1096510", "title": "Confirmation of the protective role of proteins from S. typhimurium in infection of mice with their natural pathogen.", "content": "The protective role of proteins from S. typhi-murium in infection of mice with their natural pathogens was confirmed. Hyperimmunization with either toxic or detoxified proteins, from the same batch, induced the synthesis of strongly precipitating antiprotein antibodies, and the mice were protected against infections with 15-75 LD100 of S. typhimurium. Reinfection of the same animals with 300-500 LD100 of S. typhi-murium induced, in the vaccinated mice, the synthesis of only anti-protein antibodies precipitating in agar-gel.", "contents": "Confirmation of the protective role of proteins from S. typhimurium in infection of mice with their natural pathogen. The protective role of proteins from S. typhi-murium in infection of mice with their natural pathogens was confirmed. Hyperimmunization with either toxic or detoxified proteins, from the same batch, induced the synthesis of strongly precipitating antiprotein antibodies, and the mice were protected against infections with 15-75 LD100 of S. typhimurium. Reinfection of the same animals with 300-500 LD100 of S. typhi-murium induced, in the vaccinated mice, the synthesis of only anti-protein antibodies precipitating in agar-gel."}
{"id": "PMID:1096511", "title": "[Cryptococcoma and amphotericin B. Therapy of cryptococcosis - animal experiments. 2nd Communication: Patho-histological results (author's transl)].", "content": "Since cryptococcosis is characterized by cryptococcoma formation, the antimycotic effect of amphothericin B was examined in view of such pathological-anatomical conditions. In white mice (NMRI), cryptococcoma formation was induced by intramuscular injection of Cryptococcus neoformans strain W71 into the hind leg (STAIB, 1962), using a suspension (0.2 ml) containing approximately 2.8 times 10-7 cells/ml. The mice were treated daily with 1 mg amphotericin B in 5% dimethyl sulfoxide by gastric intubation. Course of infection and effectivity of therapy were assessed by microbiological and patho-histological examination of the organs. In the present paper (2nd Communication) comparative patho-histological results in mice, treated with amphotericin B either immediately or from the 16th day p.i. or not at all, are reported. In the non-treated animals the course of infection we controlled by sacrificing 2 animals per day from the 2nd to the 25th day. Cryptococcoma found in the muscle, fat, and connective tissue in the hind leg of these animals were characterized by the two different patho-histological alterations: a) Masses of encapsulated cryptococci side by side were filling a paucireactive or non-reactive reticular structure with blood capillaries. b) Non-specific granulomatous tissue. The fungi were less abundantly found as non-encapsulated cells. On the 5th day after infection the first alterations due to dissemination were found in the lungs, then in other parenchymatous organs. Under immediate amphotericin B-therapy, no cryptococcoma was found at the place of infection; after a therapy of 30 days duration, C. neoformans could be detected in small conglomerates of non-encapsulated cells in muscle, fat and connective tissue. Histologically, a septic dissemination of the agent could not be found in this group. After a therapy of 25 days duration a shrinking of cryptococcoma was observed in animals treated from the 15th day after infection. Presumably this was caused by a loss of capsule and formation of non-specific granulomatous tissue. In the surroundings of blood vessels non-encapsulated cells were detectable. After therapy with amphotericin B, single cryptococci e.g. such disseminated into the lungs were increasingly showing morphological alterations which might be explained as forms of degeneration. The animal experiment in connection with microbiological and patho-histological follow-up studies is discussed with a view to the therapy of cryptococcosis in man. Because of the variable virulence of C. neoformans it has to be mentioned that this experiment was carried out with a strain of C. neoformans characterized by its capability to form cryptococcoma in mice.", "contents": "[Cryptococcoma and amphotericin B. Therapy of cryptococcosis - animal experiments. 2nd Communication: Patho-histological results (author's transl)]. Since cryptococcosis is characterized by cryptococcoma formation, the antimycotic effect of amphothericin B was examined in view of such pathological-anatomical conditions. In white mice (NMRI), cryptococcoma formation was induced by intramuscular injection of Cryptococcus neoformans strain W71 into the hind leg (STAIB, 1962), using a suspension (0.2 ml) containing approximately 2.8 times 10-7 cells/ml. The mice were treated daily with 1 mg amphotericin B in 5% dimethyl sulfoxide by gastric intubation. Course of infection and effectivity of therapy were assessed by microbiological and patho-histological examination of the organs. In the present paper (2nd Communication) comparative patho-histological results in mice, treated with amphotericin B either immediately or from the 16th day p.i. or not at all, are reported. In the non-treated animals the course of infection we controlled by sacrificing 2 animals per day from the 2nd to the 25th day. Cryptococcoma found in the muscle, fat, and connective tissue in the hind leg of these animals were characterized by the two different patho-histological alterations: a) Masses of encapsulated cryptococci side by side were filling a paucireactive or non-reactive reticular structure with blood capillaries. b) Non-specific granulomatous tissue. The fungi were less abundantly found as non-encapsulated cells. On the 5th day after infection the first alterations due to dissemination were found in the lungs, then in other parenchymatous organs. Under immediate amphotericin B-therapy, no cryptococcoma was found at the place of infection; after a therapy of 30 days duration, C. neoformans could be detected in small conglomerates of non-encapsulated cells in muscle, fat and connective tissue. Histologically, a septic dissemination of the agent could not be found in this group. After a therapy of 25 days duration a shrinking of cryptococcoma was observed in animals treated from the 15th day after infection. Presumably this was caused by a loss of capsule and formation of non-specific granulomatous tissue. In the surroundings of blood vessels non-encapsulated cells were detectable. After therapy with amphotericin B, single cryptococci e.g. such disseminated into the lungs were increasingly showing morphological alterations which might be explained as forms of degeneration. The animal experiment in connection with microbiological and patho-histological follow-up studies is discussed with a view to the therapy of cryptococcosis in man. Because of the variable virulence of C. neoformans it has to be mentioned that this experiment was carried out with a strain of C. neoformans characterized by its capability to form cryptococcoma in mice."}
{"id": "PMID:1096512", "title": "[A device for safe handling of vacuum-dried microbes (author's transl)].", "content": "By analogous application of the way of packing which is common for the transportation of infectious material (glass tubes in protective covers) in the handling of vaccuum-dried microbes (ampoule within the ampoule) the risk encountered when opening such receptacles may be reduced to an almost complete absence. Technical details are explained by a schematic representation. The method is suitable for the preservation of freeze-dried vaccines. All processes involved in the practical performance of the method which is ready for routine application are considered in detailed instructions for use.", "contents": "[A device for safe handling of vacuum-dried microbes (author's transl)]. By analogous application of the way of packing which is common for the transportation of infectious material (glass tubes in protective covers) in the handling of vaccuum-dried microbes (ampoule within the ampoule) the risk encountered when opening such receptacles may be reduced to an almost complete absence. Technical details are explained by a schematic representation. The method is suitable for the preservation of freeze-dried vaccines. All processes involved in the practical performance of the method which is ready for routine application are considered in detailed instructions for use."}
{"id": "PMID:1096513", "title": "[A modified immunofluorescence test to demonstrate toxoplasma antibodies (author's transl)].", "content": "A modified test to demonstrate toxoplasma antibodies by immunofluorescence is described in detail. This test was used in more than 16 000 cases. The antibody content of a patient's serum is assessed from a single dilution of 1:4 by two criteria: 1. The number of toxoplasms in the assay is kept constant. The percentage of antibody loaded cells varies with the concentration of antibodies in the patient's serum. 2. The intensity of fluorescence is correlated to the antibody content of the serum and the latter can be determined by means of standard sera. The results of this method are expressed in degrees of brightness. We compared three different methods and found an identical behaviour of the FATOX III and the Sabin-Feldman test. Between the complement-fixation test and the FATOX III there is the same relation as between JFT and SFT. This is shown in tables presenting the results for 1621 sera. The method is described in detail.", "contents": "[A modified immunofluorescence test to demonstrate toxoplasma antibodies (author's transl)]. A modified test to demonstrate toxoplasma antibodies by immunofluorescence is described in detail. This test was used in more than 16 000 cases. The antibody content of a patient's serum is assessed from a single dilution of 1:4 by two criteria: 1. The number of toxoplasms in the assay is kept constant. The percentage of antibody loaded cells varies with the concentration of antibodies in the patient's serum. 2. The intensity of fluorescence is correlated to the antibody content of the serum and the latter can be determined by means of standard sera. The results of this method are expressed in degrees of brightness. We compared three different methods and found an identical behaviour of the FATOX III and the Sabin-Feldman test. Between the complement-fixation test and the FATOX III there is the same relation as between JFT and SFT. This is shown in tables presenting the results for 1621 sera. The method is described in detail."}
{"id": "PMID:1096520", "title": "Inactivation of bacteriophages T7 and phiX174 by radiomimetics.", "content": "Inactivation of phages T7 and phiX174 induced by the radiomimetics N-nitroso-N-methylurea (NMU) and N-acetylethylenimine (AEI) has been studied. In semilogarithmic scale the kinetic curves of phage inactivation were steplike in character. This was connected with the occurrence of several processes in the system. A possible kinetic model has been proposed for the process of NMU-induced phage inactivation. The model is applicable to initial phage inactivation only. From the model a kinetic curve parameter, rho can be determined which characterizes the radiomimetic effect on different phages and determines the changes in the repair system after caffeine treatment. NMU exerted a post-inactivation effect on the phage T7. Also the NMU-induced phage T7 post-inactivation kinetic curves were of steplike character. They can be described as a sum of two exponential processes of different rate constant. The rate constant for the first stage of the post-inactivation process was close to the depurinization rate constant found in the literature.", "contents": "Inactivation of bacteriophages T7 and phiX174 by radiomimetics. Inactivation of phages T7 and phiX174 induced by the radiomimetics N-nitroso-N-methylurea (NMU) and N-acetylethylenimine (AEI) has been studied. In semilogarithmic scale the kinetic curves of phage inactivation were steplike in character. This was connected with the occurrence of several processes in the system. A possible kinetic model has been proposed for the process of NMU-induced phage inactivation. The model is applicable to initial phage inactivation only. From the model a kinetic curve parameter, rho can be determined which characterizes the radiomimetic effect on different phages and determines the changes in the repair system after caffeine treatment. NMU exerted a post-inactivation effect on the phage T7. Also the NMU-induced phage T7 post-inactivation kinetic curves were of steplike character. They can be described as a sum of two exponential processes of different rate constant. The rate constant for the first stage of the post-inactivation process was close to the depurinization rate constant found in the literature."}
{"id": "PMID:1096521", "title": "Acetylsalicylic acid in a trial to diminish thromboembolic complications after elective hip surgery.", "content": "Fifty-one patients were investigated concerning thromboembolic complications in the operated leg after elective surgery of the hip. Suspicion of clinical deep venous thrombosis (DVT) arose in 11 patients, i.e. 21 per cent. Thirty-five patients were investigated with phlebography. The incidence of DVT among those patients was 15 cases, i.e. 43 per cent. It is concluded that acetylsalicylic acid, a drug which inhibits the platelet release reaction and thereby blocks the platelet aggregation, given orally in a dose of 2 gram per day from the first postoperative day, is not effective in diminishing the incidence of postoperative deep venous thrombosis.", "contents": "Acetylsalicylic acid in a trial to diminish thromboembolic complications after elective hip surgery. Fifty-one patients were investigated concerning thromboembolic complications in the operated leg after elective surgery of the hip. Suspicion of clinical deep venous thrombosis (DVT) arose in 11 patients, i.e. 21 per cent. Thirty-five patients were investigated with phlebography. The incidence of DVT among those patients was 15 cases, i.e. 43 per cent. It is concluded that acetylsalicylic acid, a drug which inhibits the platelet release reaction and thereby blocks the platelet aggregation, given orally in a dose of 2 gram per day from the first postoperative day, is not effective in diminishing the incidence of postoperative deep venous thrombosis."}
{"id": "PMID:1096515", "title": "The contribution of endocervical smears to cervical cancer detection.", "content": "Data from a Colposcopy Clinic have been presented in which endocervical and cervical smears were evaluated in singly and in combination for accuracy and effectiveness. In patients with marked dysplasia, carcinoma in situ and early invasive cancer, false negative results rarely occurred using either cervical or endocervical smears; more false negatives were encountered in the minimal to moderate dysplasia group of lesions. Endocervical smears were found to be unreliable in determining the distribution of cervical neoplasia when correlated with endocervical curettage specimens. These smears contributed little as supplemental screening procedures for early cervical neoplasia since less procedures for early cervical neoplasia since less than three per cent of lesions would have been missed had only a cervical scrape smear been performed. It should be pointed out, however, that this was a young population in which cervical  eversion with exposure of endocervical tissue and the neoplastic lesions was the rule. The accuracy of endocervical aspiration and endocervical swab techniques was similar although there was a much higher proportion of unsatisfactory specimens with the dry cotton swab technique.", "contents": "The contribution of endocervical smears to cervical cancer detection. Data from a Colposcopy Clinic have been presented in which endocervical and cervical smears were evaluated in singly and in combination for accuracy and effectiveness. In patients with marked dysplasia, carcinoma in situ and early invasive cancer, false negative results rarely occurred using either cervical or endocervical smears; more false negatives were encountered in the minimal to moderate dysplasia group of lesions. Endocervical smears were found to be unreliable in determining the distribution of cervical neoplasia when correlated with endocervical curettage specimens. These smears contributed little as supplemental screening procedures for early cervical neoplasia since less procedures for early cervical neoplasia since less than three per cent of lesions would have been missed had only a cervical scrape smear been performed. It should be pointed out, however, that this was a young population in which cervical  eversion with exposure of endocervical tissue and the neoplastic lesions was the rule. The accuracy of endocervical aspiration and endocervical swab techniques was similar although there was a much higher proportion of unsatisfactory specimens with the dry cotton swab technique."}
{"id": "PMID:1096516", "title": "The TICAS-RTCIP real time cell identification processor.", "content": "A preliminary model of a real time processing system, the TICAS-RTCIP (for Taxonomic Intra-Cellular Analytic System--Real Time Cell Identification Processor) has been disigned and is being tested under operational conditions, proving the substantial advantages of employing a network of microprocessors operating in parallel. The system is designed only to demonstrate the feasibility, while more extensive development and biologic testing will be required to assess general applicability to automated uterine cytology.", "contents": "The TICAS-RTCIP real time cell identification processor. A preliminary model of a real time processing system, the TICAS-RTCIP (for Taxonomic Intra-Cellular Analytic System--Real Time Cell Identification Processor) has been disigned and is being tested under operational conditions, proving the substantial advantages of employing a network of microprocessors operating in parallel. The system is designed only to demonstrate the feasibility, while more extensive development and biologic testing will be required to assess general applicability to automated uterine cytology."}
{"id": "PMID:1096524", "title": "Hand reconstruction.", "content": "To conclude, one must apologize for having introduced so many subjects and left so many loose ends. Hand reconstruction poses a multitude of problems which are presently in an evolutionary phase. In discussing the notion of hand surgery specialization one draws upon orthopaedic, plastic and microsurgical techniques. But is it not precisely this adaption of diverse techniques to one organ which constitutes a speciality? Rapid progress in hand surgery was achieved the moment surgeons confined the major part of their activities to the treatment of this organ. But let us repeat that progress implies collaboration and not isolation. Advances highlighted in this review resulted mostly from collaboration between the laboratory and the operating theatre. It is worthwhile creating hand centres not only for the benefit of patients but also for research and for training surgeons. These centres must become places of collaboration between numerous clinical and preclinical specialities: namely orthopaedics, traumatology, plastic surgery, rheumatology, neurology, physiotherapy, and applicance makers, as well as biologists and anatomists, all applied to the reconstruction of the hand.", "contents": "Hand reconstruction. To conclude, one must apologize for having introduced so many subjects and left so many loose ends. Hand reconstruction poses a multitude of problems which are presently in an evolutionary phase. In discussing the notion of hand surgery specialization one draws upon orthopaedic, plastic and microsurgical techniques. But is it not precisely this adaption of diverse techniques to one organ which constitutes a speciality? Rapid progress in hand surgery was achieved the moment surgeons confined the major part of their activities to the treatment of this organ. But let us repeat that progress implies collaboration and not isolation. Advances highlighted in this review resulted mostly from collaboration between the laboratory and the operating theatre. It is worthwhile creating hand centres not only for the benefit of patients but also for research and for training surgeons. These centres must become places of collaboration between numerous clinical and preclinical specialities: namely orthopaedics, traumatology, plastic surgery, rheumatology, neurology, physiotherapy, and applicance makers, as well as biologists and anatomists, all applied to the reconstruction of the hand."}
{"id": "PMID:1096518", "title": "Preparing cell suspensions from cervical smears with pepsine and ultrasonic treatment.", "content": "Since flow-through fluorometry seemed to be a workable method for prescreening cytological material, it became important to have available a reliable method of preparing suspensions of single cells with naked nuclei. An experiment was performed with cervical smears and tissue cultures exposed to varying degrees of pepsination and ultrasonication. The results were disappointing as it appeared impossible to digest cytoplasm completely without damaging the nucleus. Ultrasonic treatment appeared to have an effect only in combination with pepsin; sonication therefore is not a useful technique for dispersion of cell clusters. Moreover, a proposal found in the literature to apply sonication to effect selective damage to leukocyte nuclei was assessed but found to be unsuccessful. Pepsin treatment and ultrasonic treatment appeared conclusively to be unreliable methods for preparing suspensions of single and naked nuclei.", "contents": "Preparing cell suspensions from cervical smears with pepsine and ultrasonic treatment. Since flow-through fluorometry seemed to be a workable method for prescreening cytological material, it became important to have available a reliable method of preparing suspensions of single cells with naked nuclei. An experiment was performed with cervical smears and tissue cultures exposed to varying degrees of pepsination and ultrasonication. The results were disappointing as it appeared impossible to digest cytoplasm completely without damaging the nucleus. Ultrasonic treatment appeared to have an effect only in combination with pepsin; sonication therefore is not a useful technique for dispersion of cell clusters. Moreover, a proposal found in the literature to apply sonication to effect selective damage to leukocyte nuclei was assessed but found to be unsuccessful. Pepsin treatment and ultrasonic treatment appeared conclusively to be unreliable methods for preparing suspensions of single and naked nuclei."}
{"id": "PMID:1096525", "title": "Injury patterns in traffic accidents and suggested preventive measures.", "content": "A short survey is given of the main patterns of injuries sustained in traffic accidents. The following injuries are accounted for: ruptures of the parasagittal bridging veins and gliding contusions of the brain, axis fractures, and aortic and pelvic injuries.", "contents": "Injury patterns in traffic accidents and suggested preventive measures. A short survey is given of the main patterns of injuries sustained in traffic accidents. The following injuries are accounted for: ruptures of the parasagittal bridging veins and gliding contusions of the brain, axis fractures, and aortic and pelvic injuries."}
{"id": "PMID:1096529", "title": "Influence of neonatal thymectomy on blood pressure and hypertensive vascular disease in rats with renal hypertension.", "content": "The effect of neonatal thymectomy on the degree and prognosis of hypertension and on the vascular lesions in rats with renal hypertension was studied. There were no differences between thymectomized and sham operated hypertensive rats. The degree of hypertension, the frequency of spontaneous death and heart infarcts were the same in both groups. The occurrence and degree of perivascular cell infiltrations, deposits of perivascular connective tissue and fibrinoid degenerations of the media were found to be the same in both the thymectomized and the sham operated hypertensive animals. The results do not support the assumption that delayed type immune reactions are important in the pathogenesis of hypertensive vascular disease in renal hypertensive rats.", "contents": "Influence of neonatal thymectomy on blood pressure and hypertensive vascular disease in rats with renal hypertension. The effect of neonatal thymectomy on the degree and prognosis of hypertension and on the vascular lesions in rats with renal hypertension was studied. There were no differences between thymectomized and sham operated hypertensive rats. The degree of hypertension, the frequency of spontaneous death and heart infarcts were the same in both groups. The occurrence and degree of perivascular cell infiltrations, deposits of perivascular connective tissue and fibrinoid degenerations of the media were found to be the same in both the thymectomized and the sham operated hypertensive animals. The results do not support the assumption that delayed type immune reactions are important in the pathogenesis of hypertensive vascular disease in renal hypertensive rats."}
{"id": "PMID:1096530", "title": "An ultrastructural study of pancreatic islet cell destruction by n-nitrosomethylurea.", "content": "An ultrastructural study of the early effects of intraperitoneal injection of 50 mg/kg body weight of N-nitrosomethylurea on the pancreatic islet tissue in Chinese hamsters was performed. Thirty minutes after injection there was slight dilatation of the endoplasmic reticulum and mitochondrial swelling in a few peripheral alpha2-cells near to capillaries and also in some beta-cells. After 1 hour these changes were more pronounced. After 3 hours, distinct nuclear pyknosis was seen in endocrine islet cells of all types together with beta-cells granular irregularities, disruption of granular cores and autophagy of alpha2-cell granules. During the following hours, marked derangement of intracellular structures, ending in obvious cellular destruction, was observed. The results indicate that N-nitrosomethylurea has a direct cytotoxic effect on the beta-, alpha2- and alpha1-cells of the islet tissue. The similarity between the ultrastructure of the pancreatic islets after injection of N-nitrosomethylurea and streptozotocin is discussed.", "contents": "An ultrastructural study of pancreatic islet cell destruction by n-nitrosomethylurea. An ultrastructural study of the early effects of intraperitoneal injection of 50 mg/kg body weight of N-nitrosomethylurea on the pancreatic islet tissue in Chinese hamsters was performed. Thirty minutes after injection there was slight dilatation of the endoplasmic reticulum and mitochondrial swelling in a few peripheral alpha2-cells near to capillaries and also in some beta-cells. After 1 hour these changes were more pronounced. After 3 hours, distinct nuclear pyknosis was seen in endocrine islet cells of all types together with beta-cells granular irregularities, disruption of granular cores and autophagy of alpha2-cell granules. During the following hours, marked derangement of intracellular structures, ending in obvious cellular destruction, was observed. The results indicate that N-nitrosomethylurea has a direct cytotoxic effect on the beta-, alpha2- and alpha1-cells of the islet tissue. The similarity between the ultrastructure of the pancreatic islets after injection of N-nitrosomethylurea and streptozotocin is discussed."}
{"id": "PMID:1096531", "title": "Quantitative studies of the renal corpuscles iii: the influence of post mortem delay before taking renal tissue samples and of the duration of tissue fixation.", "content": "Comparative quantitative studies of glomeruli in biopsies of the guenon monkey kidney were made to give a numerical measure of the effect of varying periods of fixation of the biopsy tissue in Carnoy's fluid (1-2-24 hrs.), and of the effect of progressive post mortem delay before renal tissue samples were taken (2-4-6-12 hrs.). Additional aims were to determine possible quantitative differences between subcapsular, intermediate and juxtamedullary glomeruli, and to determine whether the glomerular area should be determined with reference to the inner aspect of Bowman's capsule (total area) or to tangents laid at the top of the capillary loops (corrected total area). The quantitative methods used were total and differential counts of glomerular nuclei by light microscopy and point counting for determination of glomerular and mesangial areas. Statistical evaluation was made by a two-way analysis of variance. The differential counts of nuclei were not affected by duration of fixation, nor by post mortem delay up to 12 hrs. before tissue samples were taken. The mesangial area expressed as a percentage of total area was significantly lower two hours post mortem, while total nuclear concentration was significantly lower at time 0 and two hours post mortem than at four, six and 12 hours post mortem. Total nuclear concentration increased as duration of fixation was increased, probably a reflection of tissue shrinking. Differences in nuclear concentration were also noted at different levels of the renal cortex. These differences were only apparent when the glomerular area was determined with reference to the inner aspect of Bowman's capsule, and disappeared when the corrected total area was used. It is concluded that duration of tissue fixation and post mortem delay before tissue samples were taken both affect method sensitivity, as reflected by some, not all, of the parameters measured. The numerical differences in the parameters were not large, however, and it will be the specific study which will determine whether variable fixation times and post mortem delay in obtaining tissue samples is acceptable. As most renal biopsies contain both subcapsular and juxtamedullary glomeruli there is probably no advantage in using the corrected glomerular area instead of the total glomerular area limited by Bowman's capsule.", "contents": "Quantitative studies of the renal corpuscles iii: the influence of post mortem delay before taking renal tissue samples and of the duration of tissue fixation. Comparative quantitative studies of glomeruli in biopsies of the guenon monkey kidney were made to give a numerical measure of the effect of varying periods of fixation of the biopsy tissue in Carnoy's fluid (1-2-24 hrs.), and of the effect of progressive post mortem delay before renal tissue samples were taken (2-4-6-12 hrs.). Additional aims were to determine possible quantitative differences between subcapsular, intermediate and juxtamedullary glomeruli, and to determine whether the glomerular area should be determined with reference to the inner aspect of Bowman's capsule (total area) or to tangents laid at the top of the capillary loops (corrected total area). The quantitative methods used were total and differential counts of glomerular nuclei by light microscopy and point counting for determination of glomerular and mesangial areas. Statistical evaluation was made by a two-way analysis of variance. The differential counts of nuclei were not affected by duration of fixation, nor by post mortem delay up to 12 hrs. before tissue samples were taken. The mesangial area expressed as a percentage of total area was significantly lower two hours post mortem, while total nuclear concentration was significantly lower at time 0 and two hours post mortem than at four, six and 12 hours post mortem. Total nuclear concentration increased as duration of fixation was increased, probably a reflection of tissue shrinking. Differences in nuclear concentration were also noted at different levels of the renal cortex. These differences were only apparent when the glomerular area was determined with reference to the inner aspect of Bowman's capsule, and disappeared when the corrected total area was used. It is concluded that duration of tissue fixation and post mortem delay before tissue samples were taken both affect method sensitivity, as reflected by some, not all, of the parameters measured. The numerical differences in the parameters were not large, however, and it will be the specific study which will determine whether variable fixation times and post mortem delay in obtaining tissue samples is acceptable. As most renal biopsies contain both subcapsular and juxtamedullary glomeruli there is probably no advantage in using the corrected glomerular area instead of the total glomerular area limited by Bowman's capsule."}
{"id": "PMID:1096533", "title": "The heat production of pancreatic beta-cells stimulated by glucose.", "content": "A recently developed batch microcalorimeter was used for studying heat production in beta-cell-rich pancreatic islets isolated from obese-hyperglycemic mice. In the absence of glucose, the rate of heat production was 50 nW/islet increasing to 90 nW/islet when the islets were exposed to 20 mM glucose. The data obtained are consistent with an increase in the caloric value of oxygen with glucose concentration, as might be expected when the beta-cells utilize proportionally more carbohydrate as a source of energy.", "contents": "The heat production of pancreatic beta-cells stimulated by glucose. A recently developed batch microcalorimeter was used for studying heat production in beta-cell-rich pancreatic islets isolated from obese-hyperglycemic mice. In the absence of glucose, the rate of heat production was 50 nW/islet increasing to 90 nW/islet when the islets were exposed to 20 mM glucose. The data obtained are consistent with an increase in the caloric value of oxygen with glucose concentration, as might be expected when the beta-cells utilize proportionally more carbohydrate as a source of energy."}
{"id": "PMID:1096534", "title": "Control of the neurotoxicity of 6-hydroxydopamine by intraneuronal noradrenaline in rat iris.", "content": "In vitro studies with the neurotoxic compounds 6-hydroxydopamine (6-OH-DA) and 6-aminodopamine (6-A-DA) showed that noradrenaline (NA) markedly inhibited the autooxidation of 6-OH-DA, but not of 6-A-DA. In vivo studies of the adrenergic nerves in rat iris showed that the neurotoxic potency of 6-OH-DA, but not 6-A-DA, was increased after NA depletion by alpha-methyl-p-tyrosine methylester (H44/68). Neurotoxicity was evaluated by measuring the associated decrease in 3-H-NA uptake. Intraocular injection of NA counteracted the degenerative action of 6-OH-DA in both untreated and H44/68 pretreated rats. Intraocular NA did not interfere with the neurotoxicity of 6-A-DA. Additionally, octopamine did not affect the rate of autooxidation nor the neurotoxic potency of 6-OH-DA or 6-A-DA. Control experiments with 3-H-6-OH-DA showed that the intraneuronal NA levels did not significantly affect the intraneuronal accumulation of 6-OH-DA. The parallelism between the in vitro results on autooxidation and in vivo data on neurotoxicity makes it appear that the neurotoxic potency of 6-OH-DA and 6-A-DA is closely associated with their rates of autooxidation. The control of the degenerative action of 6-OH-DA by intraneuronal NA may be mediated via reaction of NA with radicals formed from oxygen during autooxidation of 6-OH-DA.", "contents": "Control of the neurotoxicity of 6-hydroxydopamine by intraneuronal noradrenaline in rat iris. In vitro studies with the neurotoxic compounds 6-hydroxydopamine (6-OH-DA) and 6-aminodopamine (6-A-DA) showed that noradrenaline (NA) markedly inhibited the autooxidation of 6-OH-DA, but not of 6-A-DA. In vivo studies of the adrenergic nerves in rat iris showed that the neurotoxic potency of 6-OH-DA, but not 6-A-DA, was increased after NA depletion by alpha-methyl-p-tyrosine methylester (H44/68). Neurotoxicity was evaluated by measuring the associated decrease in 3-H-NA uptake. Intraocular injection of NA counteracted the degenerative action of 6-OH-DA in both untreated and H44/68 pretreated rats. Intraocular NA did not interfere with the neurotoxicity of 6-A-DA. Additionally, octopamine did not affect the rate of autooxidation nor the neurotoxic potency of 6-OH-DA or 6-A-DA. Control experiments with 3-H-6-OH-DA showed that the intraneuronal NA levels did not significantly affect the intraneuronal accumulation of 6-OH-DA. The parallelism between the in vitro results on autooxidation and in vivo data on neurotoxicity makes it appear that the neurotoxic potency of 6-OH-DA and 6-A-DA is closely associated with their rates of autooxidation. The control of the degenerative action of 6-OH-DA by intraneuronal NA may be mediated via reaction of NA with radicals formed from oxygen during autooxidation of 6-OH-DA."}
{"id": "PMID:1096528", "title": "Observation of click-evoked compound VIII nerve responses before, during, and over seven months after kanamycin treatment in the guinea pig.", "content": "Compound VIII nerve action potential responses to clicks and filtered-clicks were recorded regularly over a period of 7 months following Kanamycin treatment (8 days, 400 mg/kg), in 3 awake guinea pigs each with an implanted electrode on the round window (left ear). From the end of the treatment the responses to the click became dissociated in various degrees within three major phases: (1) a rapid phase (8 to 10 days) where the responses were dramatically altered, (2) a recovery phase which lasted about 2 months, (3) a slow phase over the subsequent 5 months where the responses to the click decreased slowly but gradually while the N 1 component, as well as the responses to the 8 000 Hz filtered-click (which became recruiting at the end of the treatment), disappeared progressively. Responses to the low frequency filtered-clicks were less affected. Cochlear cytograms showed almost complete degeneration of the inner and outer hair cells of the first turn only.", "contents": "Observation of click-evoked compound VIII nerve responses before, during, and over seven months after kanamycin treatment in the guinea pig. Compound VIII nerve action potential responses to clicks and filtered-clicks were recorded regularly over a period of 7 months following Kanamycin treatment (8 days, 400 mg/kg), in 3 awake guinea pigs each with an implanted electrode on the round window (left ear). From the end of the treatment the responses to the click became dissociated in various degrees within three major phases: (1) a rapid phase (8 to 10 days) where the responses were dramatically altered, (2) a recovery phase which lasted about 2 months, (3) a slow phase over the subsequent 5 months where the responses to the click decreased slowly but gradually while the N 1 component, as well as the responses to the 8 000 Hz filtered-click (which became recruiting at the end of the treatment), disappeared progressively. Responses to the low frequency filtered-clicks were less affected. Cochlear cytograms showed almost complete degeneration of the inner and outer hair cells of the first turn only."}
{"id": "PMID:1096535", "title": "The effect of social class on psychiatric psychological evaluations in patients with pulmonary tuberculosis.", "content": "The present study is a methodological examination in which the social and psychic background factors of 100 tuberculous patients, aged 20 to 45 years, were explored using psychiatric interview and psychological tests (MMPI, Rorschach, and Wartegg). The purpose of the study was to analyse the effect of social background factors on the psychiatric and psychological examination. With the help of correlation coefficients and three-factor factor analysis, the variable indicating the social class of the subject could be proved to correlate significantly to the projective test variables (Rorschach, Wartegg). No such correlation between the social group variable and those obtained in the psychiatric interview or the MMPI test could be demonstrated. On the basis of the projective tests, subjects in the lower social classes were considered more disturbed. It can be supposed that the background factors characteristic of lower social classes would contribute to the unfamiliar test situation, causing reactions disturbing the test performance. This suggests that the mentioned tests should be used with caution in the evaluation of personality disturbances.", "contents": "The effect of social class on psychiatric psychological evaluations in patients with pulmonary tuberculosis. The present study is a methodological examination in which the social and psychic background factors of 100 tuberculous patients, aged 20 to 45 years, were explored using psychiatric interview and psychological tests (MMPI, Rorschach, and Wartegg). The purpose of the study was to analyse the effect of social background factors on the psychiatric and psychological examination. With the help of correlation coefficients and three-factor factor analysis, the variable indicating the social class of the subject could be proved to correlate significantly to the projective test variables (Rorschach, Wartegg). No such correlation between the social group variable and those obtained in the psychiatric interview or the MMPI test could be demonstrated. On the basis of the projective tests, subjects in the lower social classes were considered more disturbed. It can be supposed that the background factors characteristic of lower social classes would contribute to the unfamiliar test situation, causing reactions disturbing the test performance. This suggests that the mentioned tests should be used with caution in the evaluation of personality disturbances."}
{"id": "PMID:1096536", "title": "A double-blind comparison of fluphenazine decanoate and flupenthixol decanoate in the treatment of acute schizophrenia.", "content": "A double-blind comparison of fluphenazine decanoate and flupenthixol decanoate in 40 consecutive admissions showed no difference in anti-psychotic effect or extrapyramidal side effects after 56 days. However, the trial identified a different effect of the drugs on mood. Flupenthixol decanoate had an elating effect that was most marked during the week following injection. Fluphenazine decanoate tended to lower mood. The results would suggest that in acute schizophrenia, fluphenazine decanoate would be the more appropriate drug in elated or acutely disturbed patients, but that in patients with a lowered mood or a history of depression, flupenthixol decanoate would be the more appropriate drug. It was emphasised that these mood changes were observed in patients with acute schizophrenia and that extrapolation from these results to maintenance therapy of chronic relapsing schizophrenia should only be made with caution. The results suggest that 40 mg of flupenthixol decanoate is approximately equal to 25 mg of fluphenazine decanoate. Analyses of covariance showed a significant positive correlation between the incidence of extrapyramidal side effects and duration of illness.", "contents": "A double-blind comparison of fluphenazine decanoate and flupenthixol decanoate in the treatment of acute schizophrenia. A double-blind comparison of fluphenazine decanoate and flupenthixol decanoate in 40 consecutive admissions showed no difference in anti-psychotic effect or extrapyramidal side effects after 56 days. However, the trial identified a different effect of the drugs on mood. Flupenthixol decanoate had an elating effect that was most marked during the week following injection. Fluphenazine decanoate tended to lower mood. The results would suggest that in acute schizophrenia, fluphenazine decanoate would be the more appropriate drug in elated or acutely disturbed patients, but that in patients with a lowered mood or a history of depression, flupenthixol decanoate would be the more appropriate drug. It was emphasised that these mood changes were observed in patients with acute schizophrenia and that extrapolation from these results to maintenance therapy of chronic relapsing schizophrenia should only be made with caution. The results suggest that 40 mg of flupenthixol decanoate is approximately equal to 25 mg of fluphenazine decanoate. Analyses of covariance showed a significant positive correlation between the incidence of extrapyramidal side effects and duration of illness."}
{"id": "PMID:1096537", "title": "The influence of food on side effects and absorption of lithium.", "content": "In a cross-over study, 24 mmol of lithium sulphate was given as a single dose in slow release tablets to 30 healthy volunteers fasting and after a standardised meal. Comparisons were also made with lithium citrate in slow release tablets and placebo. Postprandial administration of lithium gave practically no side effects, while lithium on an empty stomach gave diarrhoea in about 20% of the subjects. The absorption was measured by determination of the amount of lithium excreted in the urine in a group of ten subjects. Lithium was completely absorbed when given after food, but when given on an empty stomach the absorption was lower in some subjects, apparently due to rapid gastrointestinal passage in connection with diarrhoea. Lithium should therefore preferably be administered after meals.", "contents": "The influence of food on side effects and absorption of lithium. In a cross-over study, 24 mmol of lithium sulphate was given as a single dose in slow release tablets to 30 healthy volunteers fasting and after a standardised meal. Comparisons were also made with lithium citrate in slow release tablets and placebo. Postprandial administration of lithium gave practically no side effects, while lithium on an empty stomach gave diarrhoea in about 20% of the subjects. The absorption was measured by determination of the amount of lithium excreted in the urine in a group of ten subjects. Lithium was completely absorbed when given after food, but when given on an empty stomach the absorption was lower in some subjects, apparently due to rapid gastrointestinal passage in connection with diarrhoea. Lithium should therefore preferably be administered after meals."}
{"id": "PMID:1096538", "title": "Does piracetam counteract the ECT-induced memory dysfunctions in depressed patients?", "content": "A double-blind, intra-individual cross-over comparison of the effect of piracetam on retrograde memory impairement as measured by the KS memory test battery was performed in connection with second and third Bi-ECT in 18 patients diagnosed as suffering from depression. The seizure duration and the post-ECT EGG patterns were examined visually and the post-ECT confusion time was measured. Piracetam was given orally in the dose of 4.8 g/day for 3 days. No significant effects were obtained on memory scores, electrical stimulus duration, EEG pattern or post-ECT confusion time. The findings may indicate that the protective effect of piracetam shown in animal electroconvuslive stimulation (ECS) is due to a counteraction of the disturbing effect of hypoxia on memory functions. It is concluded that more information is needed as regards the pharmacokinetics and the mode of action of the drug.", "contents": "Does piracetam counteract the ECT-induced memory dysfunctions in depressed patients? A double-blind, intra-individual cross-over comparison of the effect of piracetam on retrograde memory impairement as measured by the KS memory test battery was performed in connection with second and third Bi-ECT in 18 patients diagnosed as suffering from depression. The seizure duration and the post-ECT EGG patterns were examined visually and the post-ECT confusion time was measured. Piracetam was given orally in the dose of 4.8 g/day for 3 days. No significant effects were obtained on memory scores, electrical stimulus duration, EEG pattern or post-ECT confusion time. The findings may indicate that the protective effect of piracetam shown in animal electroconvuslive stimulation (ECS) is due to a counteraction of the disturbing effect of hypoxia on memory functions. It is concluded that more information is needed as regards the pharmacokinetics and the mode of action of the drug."}
{"id": "PMID:1096539", "title": "EEG in unilateral and bilateral electroconvulsive therapy.", "content": "The investigation concerned 100 patients with endogenous depression treated with ECT, 52 unilaterally on the non-dominant hemisphere, and 48 bilaterally. A double-blind investigation of the therapeutic effect and the effect on memory and on EEG was carried out. With unilateral treatment, mainly diffuse and same-sided EEG changes appeared, while with bilateral treatment mainly diffuse and left-sided changes occurred. Bilateral treatment led to significantly more EEG changes than did unilateral treatment. For both groups there was a tendency to greater changes after the last than after the sixth treatment. At termination of treatment, a significantly better therapeutic effect was found in cases where EEG changes appeared, especially severe EEG changes. This applied to the total material but not to the groups of unilaterals and bilaterals taken separately. In the entire material after the first six treatments, the greatest representation of patients with unchanged and impaired memory was found in cases with deteriorated EEG compared to cases with no EEG deterioration. After the entire series of ECT, this correlation no longer was present. After separation of the patients into unilaterals and bilaterals it was not present at any of the stages. In the bilateral group ECT treatment gave more frequent and stronger EEG changes, among the older than among the younger patients. For the unilateral group, there was no comparable difference. The number of weak seizures was significantly greater among the unilaterals than among the bilaterals. The therapeutic effect was not correlated to the number of weak seizures, but the number of weak seizures was positively correlated to the number of treatments applied.", "contents": "EEG in unilateral and bilateral electroconvulsive therapy. The investigation concerned 100 patients with endogenous depression treated with ECT, 52 unilaterally on the non-dominant hemisphere, and 48 bilaterally. A double-blind investigation of the therapeutic effect and the effect on memory and on EEG was carried out. With unilateral treatment, mainly diffuse and same-sided EEG changes appeared, while with bilateral treatment mainly diffuse and left-sided changes occurred. Bilateral treatment led to significantly more EEG changes than did unilateral treatment. For both groups there was a tendency to greater changes after the last than after the sixth treatment. At termination of treatment, a significantly better therapeutic effect was found in cases where EEG changes appeared, especially severe EEG changes. This applied to the total material but not to the groups of unilaterals and bilaterals taken separately. In the entire material after the first six treatments, the greatest representation of patients with unchanged and impaired memory was found in cases with deteriorated EEG compared to cases with no EEG deterioration. After the entire series of ECT, this correlation no longer was present. After separation of the patients into unilaterals and bilaterals it was not present at any of the stages. In the bilateral group ECT treatment gave more frequent and stronger EEG changes, among the older than among the younger patients. For the unilateral group, there was no comparable difference. The number of weak seizures was significantly greater among the unilaterals than among the bilaterals. The therapeutic effect was not correlated to the number of weak seizures, but the number of weak seizures was positively correlated to the number of treatments applied."}
{"id": "PMID:1096540", "title": "Anxiety and EEG alpha activity in neurotic patients.", "content": "The literature is reviewed on EEG alpha activity in different psychological states, i.e., morbid anxiety, attention and mental relaxation. In the present study, 20 male neurotic inpatients suffering from chronic moderate anxiety were assessed with respect to anxiety level and EEG alpha activity (frequency and per cent time) and fast activity. No significant EEG asymmetry was found. The average alpha per cent time was very low, most of the patients being low-alpha subjects. Anxiety and alpha index were significantly correlated. The patients were then treated with either 80 mg per day of temazepam or placebo for 2 weeks (double-blind). Significant effects were detected in the anxiety state, the drug group showing more improvement, but not in the EEG profile. The measures were expressed at this stage as change scores from the pre-treatment values and correlations between them were computed. The only correlation of significance was a negative correlation between anxiety level and alpha index found after 1 week in the drug group.", "contents": "Anxiety and EEG alpha activity in neurotic patients. The literature is reviewed on EEG alpha activity in different psychological states, i.e., morbid anxiety, attention and mental relaxation. In the present study, 20 male neurotic inpatients suffering from chronic moderate anxiety were assessed with respect to anxiety level and EEG alpha activity (frequency and per cent time) and fast activity. No significant EEG asymmetry was found. The average alpha per cent time was very low, most of the patients being low-alpha subjects. Anxiety and alpha index were significantly correlated. The patients were then treated with either 80 mg per day of temazepam or placebo for 2 weeks (double-blind). Significant effects were detected in the anxiety state, the drug group showing more improvement, but not in the EEG profile. The measures were expressed at this stage as change scores from the pre-treatment values and correlations between them were computed. The only correlation of significance was a negative correlation between anxiety level and alpha index found after 1 week in the drug group."}
{"id": "PMID:1096541", "title": "Peroral and parenteral administration of long-acting neuroleptics: a double-blind study of penfluridol compared to flupenthixol decanoate in the treatment of schizophrenia.", "content": "Fifty-six out of 60 schizophrenic patients completed a double-blind study of two long-acting neuroleptics, penfluridol (peroral) and flupenthixol decanoate (parenteral). Half of the patients were on maintenance therapy of flupenthixol prior to the study, the other half on penfluridol. The actual double-blind study (12 weeks) was commenced after a preliminary period of 4 weeks, the patients in the two main groups being randomly divided into two further groups, one continuing the medication unchanged, the other changing to the alternative drug. It was found possible to make a sudden switch from penfluridol to flupenthixol decanoate and vice versa without any significant change in the condition of the patient. The same dosage (in 70% of the patients from 40 to 80 mg) of penfluridol was used per week as was employed for flupenthixol decanoate per fortnight. Changes in the intensity of the symptoms (total Brief Psychiatric Rating Scale (BPRS) score) were moe pronounced in the preliminary period (during unchanged treatment) than on changed medication in the blind period. Both drugs induced approximately the same degree of akathisia, Parkinsonism and autonomic side effects. The practical consequences of equipotent therapeutical effect of a peroral and parenteral long-acting neuroleptic are briefly discussed.", "contents": "Peroral and parenteral administration of long-acting neuroleptics: a double-blind study of penfluridol compared to flupenthixol decanoate in the treatment of schizophrenia. Fifty-six out of 60 schizophrenic patients completed a double-blind study of two long-acting neuroleptics, penfluridol (peroral) and flupenthixol decanoate (parenteral). Half of the patients were on maintenance therapy of flupenthixol prior to the study, the other half on penfluridol. The actual double-blind study (12 weeks) was commenced after a preliminary period of 4 weeks, the patients in the two main groups being randomly divided into two further groups, one continuing the medication unchanged, the other changing to the alternative drug. It was found possible to make a sudden switch from penfluridol to flupenthixol decanoate and vice versa without any significant change in the condition of the patient. The same dosage (in 70% of the patients from 40 to 80 mg) of penfluridol was used per week as was employed for flupenthixol decanoate per fortnight. Changes in the intensity of the symptoms (total Brief Psychiatric Rating Scale (BPRS) score) were moe pronounced in the preliminary period (during unchanged treatment) than on changed medication in the blind period. Both drugs induced approximately the same degree of akathisia, Parkinsonism and autonomic side effects. The practical consequences of equipotent therapeutical effect of a peroral and parenteral long-acting neuroleptic are briefly discussed."}
{"id": "PMID:1096542", "title": "The effect of hydrochlorothiazide and amiloride administered together on muscle electrolytes in normal subjects.", "content": "Hydrochlorothiazide, 150 mg daily, and amiloride, 15 mg daily, have been administered together to 10 normal subjects during one week. Muscle biopsies were performed before and after the period of administration of the diuretics and the material was analysed for water and electrolytes. The body weight decreased by 1.9 kg. The serum sodium, chloride and potassium concentrations decreased significantly and standard bicarbonate and blood pH increased. In muscle tissue, extracellular water, chloride and sodium contents and intracellular sodium concentration decreased but the muscle potassium content, the intracellular potassium concentration and the muscle magnesium content were unchanged. A comparison with the results of an earlier study, in which hydrochlorothiazide was given without amiloride, showed that the intracellular potassium depletion and the increase in intracellular sodium concentration, observed with the benzothiazide diuretic, could be fully prevented by simultaneous administration of amiloride. The degree of hypokalemia and alkalosis was also smaller with hydrochlorothiazide + amiloride than with hydrochlorothiazide alone.", "contents": "The effect of hydrochlorothiazide and amiloride administered together on muscle electrolytes in normal subjects. Hydrochlorothiazide, 150 mg daily, and amiloride, 15 mg daily, have been administered together to 10 normal subjects during one week. Muscle biopsies were performed before and after the period of administration of the diuretics and the material was analysed for water and electrolytes. The body weight decreased by 1.9 kg. The serum sodium, chloride and potassium concentrations decreased significantly and standard bicarbonate and blood pH increased. In muscle tissue, extracellular water, chloride and sodium contents and intracellular sodium concentration decreased but the muscle potassium content, the intracellular potassium concentration and the muscle magnesium content were unchanged. A comparison with the results of an earlier study, in which hydrochlorothiazide was given without amiloride, showed that the intracellular potassium depletion and the increase in intracellular sodium concentration, observed with the benzothiazide diuretic, could be fully prevented by simultaneous administration of amiloride. The degree of hypokalemia and alkalosis was also smaller with hydrochlorothiazide + amiloride than with hydrochlorothiazide alone."}
{"id": "PMID:1096549", "title": "Metabolic correlates of ethanol, acetaldehyde, acetate and methanol in humans and animals.", "content": "Following the administration of alcoholic beverages, ethanol exerts a number of direct and indirect effects on the body and in turn, ethanol is itself metabolized. Liver and brain are twomajor organs which are immediately concerned with the effects of ethanol. Ethanol acts as CNS depressant and as a source of energy. Since the metabolism of ethain the liver proceeds at a constant rate until completion,acetate is produced regardless of energy requirements of the body. Thus, ethanol plays the role of an aberrant nutrient. Although ethanol has no effect on oxygen consumption in the liver, it severely suppresses the productionof carbon dioxide in the Krebs cycle resulting in the corresponding suppressionof respiratory quotient. This indicates that ethanol diverts the utilizationof oxygen for the oxidationof reducing, equivalents which accumulate as a consequence of increased formation of NADH. This is reflected in the shift from the oxidative to reductive components of a number of oxido-reductive couples, e.g.: pyruvate-lactate, oxaloacetate-malate and acetoacetate-beta-hydroxybutyrate. These actions of ethanol are exarcerbated by the fact that the metabolism of ethanol is also associated with the diversion of the availability of a number of enzymes and coenzymes from the metabolism of endogenous substrates towards the metabolism of metabolites of ethanol, thus resulting in the competitive inhibitionof a number of enzyme catalyzed reactions, e.g., inhibition of methanol metabolism during long-term consumption resulting in the acumulation of methanol in body fluids; shift in the peripheral metabolism of biogenic amines from oxidative to reductive pathways; and formation of aberrant neurotransmitters (in vitro); inhibition of the oxidation of fatty acids in the liver...", "contents": "Metabolic correlates of ethanol, acetaldehyde, acetate and methanol in humans and animals. Following the administration of alcoholic beverages, ethanol exerts a number of direct and indirect effects on the body and in turn, ethanol is itself metabolized. Liver and brain are twomajor organs which are immediately concerned with the effects of ethanol. Ethanol acts as CNS depressant and as a source of energy. Since the metabolism of ethain the liver proceeds at a constant rate until completion,acetate is produced regardless of energy requirements of the body. Thus, ethanol plays the role of an aberrant nutrient. Although ethanol has no effect on oxygen consumption in the liver, it severely suppresses the productionof carbon dioxide in the Krebs cycle resulting in the corresponding suppressionof respiratory quotient. This indicates that ethanol diverts the utilizationof oxygen for the oxidationof reducing, equivalents which accumulate as a consequence of increased formation of NADH. This is reflected in the shift from the oxidative to reductive components of a number of oxido-reductive couples, e.g.: pyruvate-lactate, oxaloacetate-malate and acetoacetate-beta-hydroxybutyrate. These actions of ethanol are exarcerbated by the fact that the metabolism of ethanol is also associated with the diversion of the availability of a number of enzymes and coenzymes from the metabolism of endogenous substrates towards the metabolism of metabolites of ethanol, thus resulting in the competitive inhibitionof a number of enzyme catalyzed reactions, e.g., inhibition of methanol metabolism during long-term consumption resulting in the acumulation of methanol in body fluids; shift in the peripheral metabolism of biogenic amines from oxidative to reductive pathways; and formation of aberrant neurotransmitters (in vitro); inhibition of the oxidation of fatty acids in the liver..."}
{"id": "PMID:1096551", "title": "Effects of ethanol on protein synthesis.", "content": "Cardiac: Cardiac protein synthesis is influenced by the state of nutrition with reduction of cardiac size in starvation. Ethanol per se may not affect this synthesis directly, but the metabolite of ethanol, acetaldehyde, profoundly decreases normal protein synthesis in the heart in vitro. The interference with the synthetic process may play a role in the ultimate cardiomyopathies of malnutrition and alcoholism. Hepatic: In vivo albumin synthesis is sensitive to environment, oncotic pressure, normal balance, nutrition, as well as toxins and state of health. Thus, to study the acute effects of alcohol alone, it was necessary to employ the isolated perfused liver. Fasting reduced albumin synthesis 50%, with loss of RNA and a disaggregation of the endoplasmic membrane bound polysome. Tryptophan, arginine and ornithine added to the perfusate at a final concentration of 10 mM reversed these findings. Alcohol likewise reduced albumin synthesis; disaggregates the bound polysome without a marked loss of RNA. Ornithine, arginine and tryptophan are able to reverse this loss in albumin synthesizing capacity. The combination of fasting and alcohol, while not lowering albumin synthesis below that seen with either stress alone, prevents the recovery from either stress.", "contents": "Effects of ethanol on protein synthesis. Cardiac: Cardiac protein synthesis is influenced by the state of nutrition with reduction of cardiac size in starvation. Ethanol per se may not affect this synthesis directly, but the metabolite of ethanol, acetaldehyde, profoundly decreases normal protein synthesis in the heart in vitro. The interference with the synthetic process may play a role in the ultimate cardiomyopathies of malnutrition and alcoholism. Hepatic: In vivo albumin synthesis is sensitive to environment, oncotic pressure, normal balance, nutrition, as well as toxins and state of health. Thus, to study the acute effects of alcohol alone, it was necessary to employ the isolated perfused liver. Fasting reduced albumin synthesis 50%, with loss of RNA and a disaggregation of the endoplasmic membrane bound polysome. Tryptophan, arginine and ornithine added to the perfusate at a final concentration of 10 mM reversed these findings. Alcohol likewise reduced albumin synthesis; disaggregates the bound polysome without a marked loss of RNA. Ornithine, arginine and tryptophan are able to reverse this loss in albumin synthesizing capacity. The combination of fasting and alcohol, while not lowering albumin synthesis below that seen with either stress alone, prevents the recovery from either stress."}
{"id": "PMID:1096553", "title": "Some endocrine aspects of alcoholism.", "content": "This review has focused on the more recent studies of ethanol-endocrine relationships. The data were evaluated from a fairly unbiased point of view and an attempt was made to point out some of the deficiencies in knowledge of the effects of ethanol on the endocrine system and the underlying mechanisms. The adrenal, for example, has been given the greatest attention in alcohol research, and yet the occurrence of atrophy or hypoplasia, despite alcohol's role as a stressor which can increase cortisol secretion, remains unexplained. In addition, our studies of the alcoholic subject have been conducted exclusively on the \"skid-row\" individual and may not be descriptive of the middle-class alcoholic subject who has not undergone similar nutritional and environmental stresses. While it is far from obvious that alcoholism is primarily an endocrine disease, the effects of ethanol on hormone metabolism are numerous and deserve further attention.", "contents": "Some endocrine aspects of alcoholism. This review has focused on the more recent studies of ethanol-endocrine relationships. The data were evaluated from a fairly unbiased point of view and an attempt was made to point out some of the deficiencies in knowledge of the effects of ethanol on the endocrine system and the underlying mechanisms. The adrenal, for example, has been given the greatest attention in alcohol research, and yet the occurrence of atrophy or hypoplasia, despite alcohol's role as a stressor which can increase cortisol secretion, remains unexplained. In addition, our studies of the alcoholic subject have been conducted exclusively on the \"skid-row\" individual and may not be descriptive of the middle-class alcoholic subject who has not undergone similar nutritional and environmental stresses. While it is far from obvious that alcoholism is primarily an endocrine disease, the effects of ethanol on hormone metabolism are numerous and deserve further attention."}
{"id": "PMID:1096554", "title": "Marihuana vs. alcohol: a pharmacologic comparison.", "content": "A review of the pharmacology, behavior, toxicity and therapeutic actions of alcohol and marihuana shows many similarities between the two drugs and few striking differences. Both drugs have fundamental non-specific actions on the neural membrane in common with the sedative-hypnotic-anesthetic group of drugs. They differ mainly in the quantitative aspects of their action owing to variation in 1) the ratio of stimulant to depressant effects, 2) their distribution in the body because of the greater lipid solubility of delta-9-tetrahydrocannabinol (delta-9THC), 3) the route of intake and 4) in their metabolic transformation. Despite marked contrasts in potency, toxicity and therapeutic potential as a result of these differences it is proposed that the proper pharmacologic classification of both drugs is in the sedative group based on their activity at non-toxic levels rather than regarding marihuana as a primary hallucinogen as presently done in many texts.", "contents": "Marihuana vs. alcohol: a pharmacologic comparison. A review of the pharmacology, behavior, toxicity and therapeutic actions of alcohol and marihuana shows many similarities between the two drugs and few striking differences. Both drugs have fundamental non-specific actions on the neural membrane in common with the sedative-hypnotic-anesthetic group of drugs. They differ mainly in the quantitative aspects of their action owing to variation in 1) the ratio of stimulant to depressant effects, 2) their distribution in the body because of the greater lipid solubility of delta-9-tetrahydrocannabinol (delta-9THC), 3) the route of intake and 4) in their metabolic transformation. Despite marked contrasts in potency, toxicity and therapeutic potential as a result of these differences it is proposed that the proper pharmacologic classification of both drugs is in the sedative group based on their activity at non-toxic levels rather than regarding marihuana as a primary hallucinogen as presently done in many texts."}
{"id": "PMID:1096555", "title": "Induction of physical dependence upon alcohol in nonhuman primates.", "content": "Following the description of the alcohol withdrawal syndrome in man, efforts have been made to use the nonhuman primate as an animal model for studying the development of physical dependence upon alcohol. There is now general agreement regarding the qualitative description of the withdrawal reaction in the rhesus monkey, and various approaches, including intravenous infusion, intragastric intubation, and nonvolitional oral intake of ethanol have been successful in producing withdrawal in these animals. There still remain the major problems of developing objective procedures for quantitatively assessing the severity of the withdrawal syndrome and establishing the dose dependent and time dependent relationships between ethanol intake and the development of physical dependence. Until these basic issues are empirically resolved, little progress can be expected in our understanding of the critical determinants of the addictive process.", "contents": "Induction of physical dependence upon alcohol in nonhuman primates. Following the description of the alcohol withdrawal syndrome in man, efforts have been made to use the nonhuman primate as an animal model for studying the development of physical dependence upon alcohol. There is now general agreement regarding the qualitative description of the withdrawal reaction in the rhesus monkey, and various approaches, including intravenous infusion, intragastric intubation, and nonvolitional oral intake of ethanol have been successful in producing withdrawal in these animals. There still remain the major problems of developing objective procedures for quantitatively assessing the severity of the withdrawal syndrome and establishing the dose dependent and time dependent relationships between ethanol intake and the development of physical dependence. Until these basic issues are empirically resolved, little progress can be expected in our understanding of the critical determinants of the addictive process."}
{"id": "PMID:1096556", "title": "Genetic determinants of alcohol addiction.", "content": "Animal and human studies indicate strain, species, and racial differences in biological responses to alcohol. Presumably, these are at least partly determined by genetic factors. However, until recently there has been little evidence suggesting that the condition called alcoholism is influenced by genetic factors. Although alcoholism is strongly familial (about one quarter of male relatives of alcoholics are themselves alcoholic), the condition is also associated with vocational and ethnic differences and at present there is no consistent evidence linking alcoholism to specific biochemical abnormalities. In recent years, however, an increasing body of evidence has suggested that genetic factors may play a role. These studies generally have been of two types: (1) twin and adoption studies, and (2) genetic marker studies. Two twin studies have been conducted. One indicated that monozygotic twins are more concordant for alcohol problems than are dizygotic twins. The other produced equivocal findings. Genetic marker studies have had inconsistent results. Of two adoption studies conducted, one found evidence for a hereditary factor and the other did not. It is hypothesized that severe forms of alcoholism may be heritable while milder forms have nongenetic origins. The virtues and limitations of the various studies are discussed.", "contents": "Genetic determinants of alcohol addiction. Animal and human studies indicate strain, species, and racial differences in biological responses to alcohol. Presumably, these are at least partly determined by genetic factors. However, until recently there has been little evidence suggesting that the condition called alcoholism is influenced by genetic factors. Although alcoholism is strongly familial (about one quarter of male relatives of alcoholics are themselves alcoholic), the condition is also associated with vocational and ethnic differences and at present there is no consistent evidence linking alcoholism to specific biochemical abnormalities. In recent years, however, an increasing body of evidence has suggested that genetic factors may play a role. These studies generally have been of two types: (1) twin and adoption studies, and (2) genetic marker studies. Two twin studies have been conducted. One indicated that monozygotic twins are more concordant for alcohol problems than are dizygotic twins. The other produced equivocal findings. Genetic marker studies have had inconsistent results. Of two adoption studies conducted, one found evidence for a hereditary factor and the other did not. It is hypothesized that severe forms of alcoholism may be heritable while milder forms have nongenetic origins. The virtues and limitations of the various studies are discussed."}
{"id": "PMID:1096557", "title": "Pathways of ethanol metabolism in perfused rat liver.", "content": "The primary pathway of hepatic ethanol metabolism involves alcohol dehydrogenase. Hydrogen generated from ethanol metabolism enters the mitochondrial space most likely as malate over a substrate shuttle mechanism, and is subsequently oxidized by the mitochondrial respiratory chain. The rate-limiting step in this overall multicompartmental process is the rate of reduced cofactor (NADH) reoxidation by the respiratory chain. Since the electron flux in the respiratory chain is controlled by the ADP supply, alcohol dehydrogenase-dependent ethanol metabolism can be activated by perturbations which circumvent the rate-limiting step, such as artificial electron acceptors, gluconeogenic precursors, and uncoupling agents. Moreover, an ATP utilizing process is responsible for the stimulation of ethanol metabilism observed following chronic pretreatment with ethanol. In perfused rat liver catalase also participates in ethanol metabolism to a lesser extent than alcohol dehydrogenase. Quantitative assessments indicate that the predominant ethanol oxidase at low ethanol concentrations (less than 20 mM) is a alcohol dehydrogenase; however, at higher ethanol concentrations, a significant portion of total ethanol metabolism (up to 50%) is mediated by catalase-hydrogen peroxide complex. This pathway is limited by the rate of generation of hydrogen peroxide in the hepatocyte, and can be stimulated with substrates for intraperoxisomal hydrogen peroxide generation such as glycolate, urate and D-amino acids. Considerable evidence implicates catalase-hydrogen peroxide complex in the mechanism of NADPH-dependent microsomal ethanol oxidation.", "contents": "Pathways of ethanol metabolism in perfused rat liver. The primary pathway of hepatic ethanol metabolism involves alcohol dehydrogenase. Hydrogen generated from ethanol metabolism enters the mitochondrial space most likely as malate over a substrate shuttle mechanism, and is subsequently oxidized by the mitochondrial respiratory chain. The rate-limiting step in this overall multicompartmental process is the rate of reduced cofactor (NADH) reoxidation by the respiratory chain. Since the electron flux in the respiratory chain is controlled by the ADP supply, alcohol dehydrogenase-dependent ethanol metabolism can be activated by perturbations which circumvent the rate-limiting step, such as artificial electron acceptors, gluconeogenic precursors, and uncoupling agents. Moreover, an ATP utilizing process is responsible for the stimulation of ethanol metabilism observed following chronic pretreatment with ethanol. In perfused rat liver catalase also participates in ethanol metabolism to a lesser extent than alcohol dehydrogenase. Quantitative assessments indicate that the predominant ethanol oxidase at low ethanol concentrations (less than 20 mM) is a alcohol dehydrogenase; however, at higher ethanol concentrations, a significant portion of total ethanol metabolism (up to 50%) is mediated by catalase-hydrogen peroxide complex. This pathway is limited by the rate of generation of hydrogen peroxide in the hepatocyte, and can be stimulated with substrates for intraperoxisomal hydrogen peroxide generation such as glycolate, urate and D-amino acids. Considerable evidence implicates catalase-hydrogen peroxide complex in the mechanism of NADPH-dependent microsomal ethanol oxidation."}
{"id": "PMID:1096570", "title": "Drug-induced dyskinesia in monkeys.", "content": "Easily definable dyskinetic syndromes have been produced by injection of L-DOPA, apomorphine, trivastal, carbachol, sodium azide, tetrahydrocannabinol, phenothiazines, and amphetamines into one or more sites. Three species of monkeys have been used: rhesus, green, and squirrel. The descriptive information available on each of these dyskinetic syndromes indicates a striking degree of similarity among the effects of these various drugs and combinations of drugs. The behavior patterns produced can be roughly divided into three areas: (1) increased movement (hyperkinesia) and vigilance, determined at least in part by the area in which the animal is confined; (2) a wide range of adventitious movements of extremities, as well as face and tongue; and (3) \"stereotypic\" behavior, sometimes destructive, such as grooming, biting, and drinking. None of the above studies or models has been employed in monkeys free-ranging in open areas with other animals. It might be expected that the effects of the drugs would be somewhat already by the social setting of the animal. It now seems adequately confirmed that the production of dyskinesia in monkeys is biochemically related to dopaminergic systems in the neostriatum. The evidence at present also points to a form of denervation hypersensitivity as the basis for the production of these syndromes in patients with dyskinesias on L-DOPA therapy (Chase, Holden, and Brody, 1972), although it is clear that in relatively high doses these drugs regularly produce dyskinesias in primates having no anatomical or biochemical pathology in the nervous system. These drug-induced dyskinesias in monkeys, especially those produced by L-DOPA in combination with other drugs or with anatomical and biochemical stereotaxically placed lesions, appear to be developed to a degree to be useful in the further evaluation of the mechanisms of dyskinesias in humans as well as providing methods of testing the effectiveness of various forms of therapy.", "contents": "Drug-induced dyskinesia in monkeys. Easily definable dyskinetic syndromes have been produced by injection of L-DOPA, apomorphine, trivastal, carbachol, sodium azide, tetrahydrocannabinol, phenothiazines, and amphetamines into one or more sites. Three species of monkeys have been used: rhesus, green, and squirrel. The descriptive information available on each of these dyskinetic syndromes indicates a striking degree of similarity among the effects of these various drugs and combinations of drugs. The behavior patterns produced can be roughly divided into three areas: (1) increased movement (hyperkinesia) and vigilance, determined at least in part by the area in which the animal is confined; (2) a wide range of adventitious movements of extremities, as well as face and tongue; and (3) \"stereotypic\" behavior, sometimes destructive, such as grooming, biting, and drinking. None of the above studies or models has been employed in monkeys free-ranging in open areas with other animals. It might be expected that the effects of the drugs would be somewhat already by the social setting of the animal. It now seems adequately confirmed that the production of dyskinesia in monkeys is biochemically related to dopaminergic systems in the neostriatum. The evidence at present also points to a form of denervation hypersensitivity as the basis for the production of these syndromes in patients with dyskinesias on L-DOPA therapy (Chase, Holden, and Brody, 1972), although it is clear that in relatively high doses these drugs regularly produce dyskinesias in primates having no anatomical or biochemical pathology in the nervous system. These drug-induced dyskinesias in monkeys, especially those produced by L-DOPA in combination with other drugs or with anatomical and biochemical stereotaxically placed lesions, appear to be developed to a degree to be useful in the further evaluation of the mechanisms of dyskinesias in humans as well as providing methods of testing the effectiveness of various forms of therapy."}
{"id": "PMID:1096575", "title": "Dopaminergic agonist effects on Parkinsonian clinical features and brain monamine metabolism.", "content": "The effect of a new dopaminergic agonist, piribedil, was studied in 16 patients with Parkinson's disease and compared with placebo and L-DOPA. Piribedil appeared to have a moderate therapeutic effect that was significantly less than that of L-DOPA. Tremor appeared to be the main clinical feature to benefit. Nausea, vomiting, and somnolence were most frequent during the buildup of treatment and confusion and hallucinations during long-term treatment. Piribedil caused a significant decrease in probenecid-induced accumulation of HVA in the CSF, suggesting reduced turnover of endogenous dopamine in the brain. There was a significant relationship between dopamine receptor activation by piribedil and improvement of parkinsonian disability.", "contents": "Dopaminergic agonist effects on Parkinsonian clinical features and brain monamine metabolism. The effect of a new dopaminergic agonist, piribedil, was studied in 16 patients with Parkinson's disease and compared with placebo and L-DOPA. Piribedil appeared to have a moderate therapeutic effect that was significantly less than that of L-DOPA. Tremor appeared to be the main clinical feature to benefit. Nausea, vomiting, and somnolence were most frequent during the buildup of treatment and confusion and hallucinations during long-term treatment. Piribedil caused a significant decrease in probenecid-induced accumulation of HVA in the CSF, suggesting reduced turnover of endogenous dopamine in the brain. There was a significant relationship between dopamine receptor activation by piribedil and improvement of parkinsonian disability."}
{"id": "PMID:1096580", "title": "The use of an interactive computer terminal in the assessment of cognitive function in elderly psychiatric patients.", "content": "The use of a teaching machine is the assessment of mental function has been described by Gredye and his colleagues. This report concerns its use in testing 56 elderly patients suffering from functional psychiatric disorders of dementia. After a diagnostic interview patients were tested on the teaching machine as well as on the Mill Hill, Progressive Matrices, Paired Associate Learning Test and Digit Copying Test. After a fixed period all tests were repeated and clincal progress rated. Results showed a significant correlation (P less than 0.01) between teaching-machine scores and those on the standard psychological tests. The correlation between the machine scores on the two occasions was high (r equals 0.58) but this was complicated by practice effects and by low 'ceiling' of the test. It is concluded that the test provided a relatively reliable and valid measure of cognitive function but that its value would be increased by providing a parallel form and by extending its level of difficulty.", "contents": "The use of an interactive computer terminal in the assessment of cognitive function in elderly psychiatric patients. The use of a teaching machine is the assessment of mental function has been described by Gredye and his colleagues. This report concerns its use in testing 56 elderly patients suffering from functional psychiatric disorders of dementia. After a diagnostic interview patients were tested on the teaching machine as well as on the Mill Hill, Progressive Matrices, Paired Associate Learning Test and Digit Copying Test. After a fixed period all tests were repeated and clincal progress rated. Results showed a significant correlation (P less than 0.01) between teaching-machine scores and those on the standard psychological tests. The correlation between the machine scores on the two occasions was high (r equals 0.58) but this was complicated by practice effects and by low 'ceiling' of the test. It is concluded that the test provided a relatively reliable and valid measure of cognitive function but that its value would be increased by providing a parallel form and by extending its level of difficulty."}
{"id": "PMID:1096583", "title": "Calibration standards for counting asbestos.", "content": "A laboratory proficiency testing program has been initiated by NIOSH to permit standardization of asbestos counting procedures by various state agencies. This has required preparation of multiple membrane filter samples containing \"identical and predictable\" concentrations of asbestos fibers, with consistent particulate backgrounds. A technique to filter liquid (toluene) suspensions was developed to provide chrysotile asbestos standards ranging from 200 to 1,500 fibers/mm-2 of filter area, with an aluminum oxide particulate background. Details of the necessary techniques are described. To evaluate variations inherent in preparing these \"identical\" samples and to estimate variations between different counters, extensive replicate fiber counting was performed. Count data from several counters at a single facility show that this liquid suspension filtration technique provides reproducible asbestos standards as measured by optical microscopy with a coefficient of variation of plus or minus 20% with the maximum individual variations of plus or minus 50%.", "contents": "Calibration standards for counting asbestos. A laboratory proficiency testing program has been initiated by NIOSH to permit standardization of asbestos counting procedures by various state agencies. This has required preparation of multiple membrane filter samples containing \"identical and predictable\" concentrations of asbestos fibers, with consistent particulate backgrounds. A technique to filter liquid (toluene) suspensions was developed to provide chrysotile asbestos standards ranging from 200 to 1,500 fibers/mm-2 of filter area, with an aluminum oxide particulate background. Details of the necessary techniques are described. To evaluate variations inherent in preparing these \"identical\" samples and to estimate variations between different counters, extensive replicate fiber counting was performed. Count data from several counters at a single facility show that this liquid suspension filtration technique provides reproducible asbestos standards as measured by optical microscopy with a coefficient of variation of plus or minus 20% with the maximum individual variations of plus or minus 50%."}
{"id": "PMID:1096584", "title": "Technological feasibility of the 2 fibers/cc asbestos standard in asbestos textile facilities.", "content": "The technical feasibility of developing dust control practice which would bring the asbestos industry into compliance with the OSHA I fibers/cc standard, scheduled to become effective July 1, 1976, has been questioned since its promulgation. This paper presents the results of industrial hygiene surveys, including the results of 243 asbestos air samples, conducted at two asbestos textile production facilities which have achieved the 2 fibers/cc standard. Both facilities use nonconventional production techniques. Conclusions drawn from the investigations express the need for: (1) a study of new products; (2) a tool to motivate consumers to switch to the new products when appropriate; and (3) legislation which will protect all domestic producers of asbestos textiles from certain producers who do not have to absorb the added expense of meeting the OSHA standard.", "contents": "Technological feasibility of the 2 fibers/cc asbestos standard in asbestos textile facilities. The technical feasibility of developing dust control practice which would bring the asbestos industry into compliance with the OSHA I fibers/cc standard, scheduled to become effective July 1, 1976, has been questioned since its promulgation. This paper presents the results of industrial hygiene surveys, including the results of 243 asbestos air samples, conducted at two asbestos textile production facilities which have achieved the 2 fibers/cc standard. Both facilities use nonconventional production techniques. Conclusions drawn from the investigations express the need for: (1) a study of new products; (2) a tool to motivate consumers to switch to the new products when appropriate; and (3) legislation which will protect all domestic producers of asbestos textiles from certain producers who do not have to absorb the added expense of meeting the OSHA standard."}
{"id": "PMID:1096586", "title": "Background documentation of evaluation of occupational exposure to airborne asbestos.", "content": "This review presents background information and literature documentation to supplement the \"Recommended Procedures for Sampling and Counting Asbestos Fibers: Procedures for the Evaluation of Occupational Exposure to Airborne Asbestos\" prepared by the joint ACGIH-AIHA Aerosol Hazards Evaluation Committee. It reviews the nature of the inhalation hazard associated with asbesots fibers, the sampling and analytic methods which have been used, and a rationale for the selection of the membrane filter sampling-optical phase microscope identification and assay methodology which is recommended.", "contents": "Background documentation of evaluation of occupational exposure to airborne asbestos. This review presents background information and literature documentation to supplement the \"Recommended Procedures for Sampling and Counting Asbestos Fibers: Procedures for the Evaluation of Occupational Exposure to Airborne Asbestos\" prepared by the joint ACGIH-AIHA Aerosol Hazards Evaluation Committee. It reviews the nature of the inhalation hazard associated with asbesots fibers, the sampling and analytic methods which have been used, and a rationale for the selection of the membrane filter sampling-optical phase microscope identification and assay methodology which is recommended."}
{"id": "PMID:1096587", "title": "A simple method for anzlyzing multifactorial data.", "content": "A simple method of isolating significant factors from multifactorial data has been outlined. The factors must be at two levels, but any number of factors can be handled. Variable group sizes and empty groups do not invalidate the method.", "contents": "A simple method for anzlyzing multifactorial data. A simple method of isolating significant factors from multifactorial data has been outlined. The factors must be at two levels, but any number of factors can be handled. Variable group sizes and empty groups do not invalidate the method."}
{"id": "PMID:1096588", "title": "Effect of microorganisms isolated from the upper gut of malnourished children on intestinal sugar absorption in vivo.", "content": "The effect of microorganisms isolated from the upper gastrointestinal tract of malnourished children on intestinal sugar absorption was studied in rats in vivo. Pure cultures of organisms were grown overnight in a nutrient broth and the resultant supernatant fluid which contained microorganisms in similar numbers to those found in the patients was used as the basic solution for jejunal perfusions which were done in anesthetized adult Wistar rats. The substrate used was arbutin (p-hydroxphenyl-beta-glucoside), a recognized marker of intestinal active sugar transport. Of the gram-positive cocci studied, only the saprophyte, Staphylococcus saprophyticus, did not adversely affect the intestinal absorption of arbutin. The only gram-positive rod studied, a lactobacillus, also significantly inhibited arbutin absorption. Of the Enterobacteriaciae studied, Salmonella paratyphi B, a Shigella and Proteus sp. did not affect arbutin absorption. All the species of Escherichia coli studied, including a nonpathogenic variety, inhibited absorption. Klebsiella sp. and Pseudomonas sp. were also effective. Of the Candida sp., C. albicans and C. parapsilosis were inhibitory while C. tropicalis was not. These results suggest that microorganisms not generally considered enteropathogenic may adversely affect intestinal function when present in the lumen of the gut in excessive numbers and contribute to the production of diarrhea in children with malnutrition.", "contents": "Effect of microorganisms isolated from the upper gut of malnourished children on intestinal sugar absorption in vivo. The effect of microorganisms isolated from the upper gastrointestinal tract of malnourished children on intestinal sugar absorption was studied in rats in vivo. Pure cultures of organisms were grown overnight in a nutrient broth and the resultant supernatant fluid which contained microorganisms in similar numbers to those found in the patients was used as the basic solution for jejunal perfusions which were done in anesthetized adult Wistar rats. The substrate used was arbutin (p-hydroxphenyl-beta-glucoside), a recognized marker of intestinal active sugar transport. Of the gram-positive cocci studied, only the saprophyte, Staphylococcus saprophyticus, did not adversely affect the intestinal absorption of arbutin. The only gram-positive rod studied, a lactobacillus, also significantly inhibited arbutin absorption. Of the Enterobacteriaciae studied, Salmonella paratyphi B, a Shigella and Proteus sp. did not affect arbutin absorption. All the species of Escherichia coli studied, including a nonpathogenic variety, inhibited absorption. Klebsiella sp. and Pseudomonas sp. were also effective. Of the Candida sp., C. albicans and C. parapsilosis were inhibitory while C. tropicalis was not. These results suggest that microorganisms not generally considered enteropathogenic may adversely affect intestinal function when present in the lumen of the gut in excessive numbers and contribute to the production of diarrhea in children with malnutrition."}
{"id": "PMID:1096589", "title": "Richter's syndrome. A terminal complication of chronic lymphocytic leukemia with distinct clinicopathologic features.", "content": "Long, John C., AND Aisenberg, Alan C.: Richter's syndrome. A terminal complication of chronic lymphocytic leukemia with distinct clinicopathologic features. Am J Clin Pathol 63: 786-795, 1975. Two patients with chronic lymphocytic leukemia of 8 and 9 years duration had a terminal illness characterized by weight loss, persistent fever, lymphadenopathy, and dysglobulinemia. In both cases autopsy revealed a pleomorphic histiocytic lymphoma which contained multinucleate tumor cells and was associated with persistent chronic lymphocytic leukemia. These two cases are examples of Richter's syndrome, a clinically distinct complication of chronic lymphocytic leukemia that may be confused pathologically with Hodgkin's disease. Review of the medical literature suggests that this syndrome has frequently gone unrecognized. (Key words: Richter's syndrome; Chronic lymphocytic leukemia.)", "contents": "Richter's syndrome. A terminal complication of chronic lymphocytic leukemia with distinct clinicopathologic features. Long, John C., AND Aisenberg, Alan C.: Richter's syndrome. A terminal complication of chronic lymphocytic leukemia with distinct clinicopathologic features. Am J Clin Pathol 63: 786-795, 1975. Two patients with chronic lymphocytic leukemia of 8 and 9 years duration had a terminal illness characterized by weight loss, persistent fever, lymphadenopathy, and dysglobulinemia. In both cases autopsy revealed a pleomorphic histiocytic lymphoma which contained multinucleate tumor cells and was associated with persistent chronic lymphocytic leukemia. These two cases are examples of Richter's syndrome, a clinically distinct complication of chronic lymphocytic leukemia that may be confused pathologically with Hodgkin's disease. Review of the medical literature suggests that this syndrome has frequently gone unrecognized. (Key words: Richter's syndrome; Chronic lymphocytic leukemia.)"}
{"id": "PMID:1096590", "title": "Erosive gastritis as a manifestation of secondary syphilis.", "content": "In a case of secondary syphilis with gastric involvement, the clinical history and roentgenographic and gastroscopic examiniations initially suggested an infiltrative malignant neoplasm. However, macroscopic and histologic examination of the partial gastrectomy specimen demonstrated an erosive, inflammatory lesion. Subsequently, numerous Treponema pallida were demonstrated in formalin-fixed eroded gastric mucosa by silver impregnation and fluorescent antibody technics. Although the true incidence of syphilitic gastritis is unknown, it may occur commonly during the secondary stage of syphilis and should be considered in the differential diagnosis of diffuse erosive gastritides and infiltrative lesions of the stomach.", "contents": "Erosive gastritis as a manifestation of secondary syphilis. In a case of secondary syphilis with gastric involvement, the clinical history and roentgenographic and gastroscopic examiniations initially suggested an infiltrative malignant neoplasm. However, macroscopic and histologic examination of the partial gastrectomy specimen demonstrated an erosive, inflammatory lesion. Subsequently, numerous Treponema pallida were demonstrated in formalin-fixed eroded gastric mucosa by silver impregnation and fluorescent antibody technics. Although the true incidence of syphilitic gastritis is unknown, it may occur commonly during the secondary stage of syphilis and should be considered in the differential diagnosis of diffuse erosive gastritides and infiltrative lesions of the stomach."}
{"id": "PMID:1096591", "title": "Toxoplasmosis. Historical review, direct diagnostic microscopy, and report of a case.", "content": "A case of congenital toxoplasmosis diagnosed by the detection of Toxoplasma gondii organisms in the ventricular fluid of a living patient, confirmed by mouse inoculation, is reported. The subject of toxoplasmosis is reviewed historically, especially in regard to cases diagnosed by direct microscopic examination of cerebrospinal or ventricular fluids. Although several such cases have been reported in the world medical literature, an extensive survey failed to reveal such a case previously reported in the United States.", "contents": "Toxoplasmosis. Historical review, direct diagnostic microscopy, and report of a case. A case of congenital toxoplasmosis diagnosed by the detection of Toxoplasma gondii organisms in the ventricular fluid of a living patient, confirmed by mouse inoculation, is reported. The subject of toxoplasmosis is reviewed historically, especially in regard to cases diagnosed by direct microscopic examination of cerebrospinal or ventricular fluids. Although several such cases have been reported in the world medical literature, an extensive survey failed to reveal such a case previously reported in the United States."}
{"id": "PMID:1096592", "title": "Recovery of Cryptococcus neoformans from sputum using new technics for the isolation of fungi from sputum.", "content": "Three sputum-digesting agents, N-acetyl-l-cytseine, dithiothreitol, and pancreatin-trypsin, were shown to be equally effective in allowing for the isolation of Cryptococcus neoformans from sputum samples in quantitative comparisons.  By quantitative plating on bird-seed medium it was also shown the centrifugation after digestion concentrated C. neoformans into a platable sediment and, further, that the organisms, when present in concentrations as low as 10 yeasts per ml. of sputum, could be isolated with much higher frequencies than when no digestion-centrifugation procedure was used.", "contents": "Recovery of Cryptococcus neoformans from sputum using new technics for the isolation of fungi from sputum. Three sputum-digesting agents, N-acetyl-l-cytseine, dithiothreitol, and pancreatin-trypsin, were shown to be equally effective in allowing for the isolation of Cryptococcus neoformans from sputum samples in quantitative comparisons.  By quantitative plating on bird-seed medium it was also shown the centrifugation after digestion concentrated C. neoformans into a platable sediment and, further, that the organisms, when present in concentrations as low as 10 yeasts per ml. of sputum, could be isolated with much higher frequencies than when no digestion-centrifugation procedure was used."}
{"id": "PMID:1096593", "title": "The accuracy of calcium analysis in the United States.", "content": "The accuracy of the methods commonly used for calcium analysis in clinical laboratories in the United States was studied with the aid of the National Bureau of Standards (NBS). Samples from six large serum pools that had been analyzed by 4,258 clinical laboratories were sent to NBS. At NBS the calcium contents of the samples were determined using isotope dilution and mass spectrometry (ID-MS). The findings show that the bias of the routine methods varies from levels that are insignificant to levels that would have an impact on patient care. The accuracy of the automated methods is comparable to the accuracy of the best manual methods. The accuracy of the proposed calcium reference method is comparable to the accuracy of a number of the methods in current routine use. There is evidence of a widespread lack of standardization at the extremes of the clinical range; this effect is not shown by the results obtained by automated methods. The study underlines the value of developing technology, such as ID-MS, that is capable of providing definitive values. The definitive values can then be used to assess the bias of routine methods on a nationwide scale, using existing programs.", "contents": "The accuracy of calcium analysis in the United States. The accuracy of the methods commonly used for calcium analysis in clinical laboratories in the United States was studied with the aid of the National Bureau of Standards (NBS). Samples from six large serum pools that had been analyzed by 4,258 clinical laboratories were sent to NBS. At NBS the calcium contents of the samples were determined using isotope dilution and mass spectrometry (ID-MS). The findings show that the bias of the routine methods varies from levels that are insignificant to levels that would have an impact on patient care. The accuracy of the automated methods is comparable to the accuracy of the best manual methods. The accuracy of the proposed calcium reference method is comparable to the accuracy of a number of the methods in current routine use. There is evidence of a widespread lack of standardization at the extremes of the clinical range; this effect is not shown by the results obtained by automated methods. The study underlines the value of developing technology, such as ID-MS, that is capable of providing definitive values. The definitive values can then be used to assess the bias of routine methods on a nationwide scale, using existing programs."}
{"id": "PMID:1096594", "title": "Treatment of laryngotracheobronchitis (croup). Use of intermittent positive-pressure breathing and racemic epinephrine.", "content": "Use of racemic epinephrine hydrochloride (Vaponefrin), delivered by intermittent positive-pressure breathing, in the treatment of laryngotracheobronchitis (croup) produced acute beneficial results in a controlled study. However, symptoms often recurred within two hours, suggesting that this form of treatment should not be used in the emergency room and the patient then sent home. This treatment had no effect on arterial oxygen gas pressure. The changes in clinical status 24 to 36 hours after admission into the study were similar for the patients in the treatment and control groups, suggesting that the natural history of the disease was not drastically altered by this form of treatment.", "contents": "Treatment of laryngotracheobronchitis (croup). Use of intermittent positive-pressure breathing and racemic epinephrine. Use of racemic epinephrine hydrochloride (Vaponefrin), delivered by intermittent positive-pressure breathing, in the treatment of laryngotracheobronchitis (croup) produced acute beneficial results in a controlled study. However, symptoms often recurred within two hours, suggesting that this form of treatment should not be used in the emergency room and the patient then sent home. This treatment had no effect on arterial oxygen gas pressure. The changes in clinical status 24 to 36 hours after admission into the study were similar for the patients in the treatment and control groups, suggesting that the natural history of the disease was not drastically altered by this form of treatment."}
{"id": "PMID:1096595", "title": "Flouride. Ten-year prospective study of deciduous and permanent dentition.", "content": "A ten-year longitudinal prospective study compared the effect of fluoride on dentition of 1,500 children from infancy through age 10 years. In Kalamazoo, Mich, and Oneida, NY, parallel groups were given a fluoride-vitamin supplement from infancy and from age 4 and compared with fluoridated water and control groups. Incidence of new caries activity in both deciduous and permanent teeth was measured by mean number of new decayed and filled teeth, as well as percentage of children with no caries throughout the periods studied. Prevalence of caries was also studied in six-year molars. The results indicate a consistent (and, for selected groups, a statistically significant) diminution in caries activity for both deciduous and permanent teeth for groups ranked from greatest retardation of caries to least: infant fluoride group, water fluoride group, age 4 fluoride group, and controls.", "contents": "Flouride. Ten-year prospective study of deciduous and permanent dentition. A ten-year longitudinal prospective study compared the effect of fluoride on dentition of 1,500 children from infancy through age 10 years. In Kalamazoo, Mich, and Oneida, NY, parallel groups were given a fluoride-vitamin supplement from infancy and from age 4 and compared with fluoridated water and control groups. Incidence of new caries activity in both deciduous and permanent teeth was measured by mean number of new decayed and filled teeth, as well as percentage of children with no caries throughout the periods studied. Prevalence of caries was also studied in six-year molars. The results indicate a consistent (and, for selected groups, a statistically significant) diminution in caries activity for both deciduous and permanent teeth for groups ranked from greatest retardation of caries to least: infant fluoride group, water fluoride group, age 4 fluoride group, and controls."}
{"id": "PMID:1096597", "title": "Stimulation of human pepsin output by tropical hydrochloric acid.", "content": "Pepsin secretion is stimulated by the back-diffusion of acid across the mucosa of the vagally denervated canine pouch.  If back-diffusion is enhanced by damage, pepsin secretion increases.  The current study investigates whether this mechanism exists in man.  The stomach of normal human volunteers were irrigated for 1 hour with either buffer of 0.01 N HCl, 1 hour with 0.2 N HCl, and a final hour with buffer or 0.01 N HCl.  During the middle hour both the concentration and output of pepsin increased three- or fourfold.  From these studies it appears that the human gastric mucosa contains a mechanism similar to the dog's which results in the stimulation of pepsin secretion when exposed to acid.  This mechanism could be of etiologic significance in gastric ulcer disease, which has been shown to be associated with increased gastric-mucosal permeability.", "contents": "Stimulation of human pepsin output by tropical hydrochloric acid. Pepsin secretion is stimulated by the back-diffusion of acid across the mucosa of the vagally denervated canine pouch.  If back-diffusion is enhanced by damage, pepsin secretion increases.  The current study investigates whether this mechanism exists in man.  The stomach of normal human volunteers were irrigated for 1 hour with either buffer of 0.01 N HCl, 1 hour with 0.2 N HCl, and a final hour with buffer or 0.01 N HCl.  During the middle hour both the concentration and output of pepsin increased three- or fourfold.  From these studies it appears that the human gastric mucosa contains a mechanism similar to the dog's which results in the stimulation of pepsin secretion when exposed to acid.  This mechanism could be of etiologic significance in gastric ulcer disease, which has been shown to be associated with increased gastric-mucosal permeability."}
{"id": "PMID:1096599", "title": "Hemorrhagic pancreatitis with duodenopancreatic fistula in a renal homograft patient.", "content": "A 39-year old man who developed hemorrhagic pancreatitis seven months after his second renal homograft survived. Azathioprine was stopped but prednisone was continued. A pseudocyst spontaneously vanished and an upper gastrointestinal series showed a duodenopancreatic fistula. His homograft maintained good function.", "contents": "Hemorrhagic pancreatitis with duodenopancreatic fistula in a renal homograft patient. A 39-year old man who developed hemorrhagic pancreatitis seven months after his second renal homograft survived. Azathioprine was stopped but prednisone was continued. A pseudocyst spontaneously vanished and an upper gastrointestinal series showed a duodenopancreatic fistula. His homograft maintained good function."}
{"id": "PMID:1096608", "title": "The \"fetoscope\"--a new clinical tool for prenatal genetic diagnosis.", "content": "Our experience in 65 patients using a \"fetoscope\" and local anesthesia prior to saline abortion is described. Visualization of the intrauterine contents improved with experience, was optimum at 16 to 18 weeks' gestation, and was superior to the quality of photographic records. Four fetal skin biopsies and 6 amnion biopsies were done. Six blood samples were taken from the fetal surface of the placenta. The limitations of this instrument are described. No significant complications have occurred. Current and probable future indications for fetoscopy are reviewed. Until both the safety of the procedure is proved and problems of visualization due to limitation of visual field are solved, extreme caution is urged in the employment of this instrument in genetic high-risk pregnancy.", "contents": "The \"fetoscope\"--a new clinical tool for prenatal genetic diagnosis. Our experience in 65 patients using a \"fetoscope\" and local anesthesia prior to saline abortion is described. Visualization of the intrauterine contents improved with experience, was optimum at 16 to 18 weeks' gestation, and was superior to the quality of photographic records. Four fetal skin biopsies and 6 amnion biopsies were done. Six blood samples were taken from the fetal surface of the placenta. The limitations of this instrument are described. No significant complications have occurred. Current and probable future indications for fetoscopy are reviewed. Until both the safety of the procedure is proved and problems of visualization due to limitation of visual field are solved, extreme caution is urged in the employment of this instrument in genetic high-risk pregnancy."}
{"id": "PMID:1096609", "title": "Preinduction priming with oral prostaglandin E2.", "content": "A double-blind study was undertaken to determine the effectiveness of prostaglandin E2 as a means for improving the prelabor pelvic conditions preparatory to induction of labor at term in 30 patients with unfavorable Bishop scores of less than 6. An interval of priming was followed after 8 to 12 hours by induction with oxytocin infusion. No meaningful differences were found in index cases as compared with controls to verify that PGE2 has any priming or enhancing effect under the conditions of the investigation.", "contents": "Preinduction priming with oral prostaglandin E2. A double-blind study was undertaken to determine the effectiveness of prostaglandin E2 as a means for improving the prelabor pelvic conditions preparatory to induction of labor at term in 30 patients with unfavorable Bishop scores of less than 6. An interval of priming was followed after 8 to 12 hours by induction with oxytocin infusion. No meaningful differences were found in index cases as compared with controls to verify that PGE2 has any priming or enhancing effect under the conditions of the investigation."}
{"id": "PMID:1096603", "title": "Clinical applications of complement measurements in rheumatic diseases.", "content": "There is now convincing evidence that the complement system is involved in the pathogenesis of at least some of the manifestations of human rheumatic diseases.  Complement measurements in serum and/or pathologic fluids from patients with these disorders not only reflect this involvement but also may provide important clues regarding the activity and extent of the disease processes.  Future studies should provide additional information concerning the usefulness of such measurements for predicting the outcome of specific therapeutic regimens, and perhaps also be the basis for the evolution of new and more rational forms of therapy.", "contents": "Clinical applications of complement measurements in rheumatic diseases. There is now convincing evidence that the complement system is involved in the pathogenesis of at least some of the manifestations of human rheumatic diseases.  Complement measurements in serum and/or pathologic fluids from patients with these disorders not only reflect this involvement but also may provide important clues regarding the activity and extent of the disease processes.  Future studies should provide additional information concerning the usefulness of such measurements for predicting the outcome of specific therapeutic regimens, and perhaps also be the basis for the evolution of new and more rational forms of therapy."}
{"id": "PMID:1096604", "title": "The demands and limits of care--ethical reflections on the moral dilemma of neonatal intensive care.", "content": "Decisions regarding the care of deformed and retarded infants pose difficult ethical and moral dilemmas for both physicians and parents.  Ethical inquiry regarding such questions must be concerned with how we see and understand the dilemma for conceptualization of the problem often shapes the decisions that are made.  The fundamental issue that provides the background for the kind and amount of care defective newborns should receive is the obligation parents have to care for their children.  New medical technology has so extended the limits of care that the extent of parental (and societal) obligation is unclear.  In dealing with the problem of defective newborns, the action of both physicians and parents is determined in part by concern to act in a manner congruent with assumed roles and identity.  With no moral consensus regarding the care of defective newborns, the doctor is put in a precarious position.  What is revealed is how dependent medicine is on its underlying moral community to give it moral direction in instances of ambiguity and uncertainty.", "contents": "The demands and limits of care--ethical reflections on the moral dilemma of neonatal intensive care. Decisions regarding the care of deformed and retarded infants pose difficult ethical and moral dilemmas for both physicians and parents.  Ethical inquiry regarding such questions must be concerned with how we see and understand the dilemma for conceptualization of the problem often shapes the decisions that are made.  The fundamental issue that provides the background for the kind and amount of care defective newborns should receive is the obligation parents have to care for their children.  New medical technology has so extended the limits of care that the extent of parental (and societal) obligation is unclear.  In dealing with the problem of defective newborns, the action of both physicians and parents is determined in part by concern to act in a manner congruent with assumed roles and identity.  With no moral consensus regarding the care of defective newborns, the doctor is put in a precarious position.  What is revealed is how dependent medicine is on its underlying moral community to give it moral direction in instances of ambiguity and uncertainty."}
{"id": "PMID:1096612", "title": "Uterine contractility and regional blood flow responses to oxytocin and prostaglandin E2 in pregnant rhesus monkeys.", "content": "The effects of oxytocin and prostaglandin E2 (PGE2) infusions on regional blood flow (measured by radioactive microspheres) and myometrial contractility were studied in 18 pregnant rhesus monkeys near term. We observed no significant differences between PGE2 and oxytocin in cardiac output, hemodynamics, or uterine activity. Their effects on the regional distribution of systemic blood flow were similar although the gastrointestinal tract received an increased percentage of cardiac output after PGE2. A relative placental ischemia, together with a myometrial hyperemia, was observed during labor. Uterine contraction produced a large reduction (average 73 per cent) in placental blood flow whereas myometrial blood flow was maintained or sometimes increased. A significant negative correlation was observed between intra-amniotic pressure and placental blood flow. During uterine relaxation, placental flow partially recovered whereas myometrial flow nearly doubled the prelabor values. We conclude that (1) in the dose ranges studied, oxytocin and PGE2 produce similar effects on myometrial contractility and uteroplacental blood flow, and (2) the vascular beds of the placenta and myometrium respond differently to labor of moderate intensity.", "contents": "Uterine contractility and regional blood flow responses to oxytocin and prostaglandin E2 in pregnant rhesus monkeys. The effects of oxytocin and prostaglandin E2 (PGE2) infusions on regional blood flow (measured by radioactive microspheres) and myometrial contractility were studied in 18 pregnant rhesus monkeys near term. We observed no significant differences between PGE2 and oxytocin in cardiac output, hemodynamics, or uterine activity. Their effects on the regional distribution of systemic blood flow were similar although the gastrointestinal tract received an increased percentage of cardiac output after PGE2. A relative placental ischemia, together with a myometrial hyperemia, was observed during labor. Uterine contraction produced a large reduction (average 73 per cent) in placental blood flow whereas myometrial blood flow was maintained or sometimes increased. A significant negative correlation was observed between intra-amniotic pressure and placental blood flow. During uterine relaxation, placental flow partially recovered whereas myometrial flow nearly doubled the prelabor values. We conclude that (1) in the dose ranges studied, oxytocin and PGE2 produce similar effects on myometrial contractility and uteroplacental blood flow, and (2) the vascular beds of the placenta and myometrium respond differently to labor of moderate intensity."}
{"id": "PMID:1096615", "title": "Changes in LH-releasing hormone content of the hypothalamus and electron microscopy of the anterior pituitary after prostaglandin E2 injection in rats.", "content": "The purpose of this study is to present evidence that prostaglandin (PG) E2 stimulates a release of luteinizing hormone-releasing hormone (LH-RH) from the hypothalmus. In experiment 1, 72 hours after estrogen and progesterone treatment, a solution of 700 mug of PGE2 was injected into the jugular vein. Blood samples for plasma LH and the hypothalamus for LH-RH assay were collected before and 5, 10, and 30 minutes after PGE2 injection and subjected to radioimmunoassay. The total LH-RH content of the hypothalamus decreased from 20.4 to 15.3 ng. at 5 minutes after PGE2 and had returned to the pretreatment level by 30 minutes after PGE2 administration. On the other hand, plasma LH levels increased at 5 minutes, reached a peak at 10 minutes, followed by a decline at 30 minutes after PGE2 injection. In Experiment 2, 10 and 30 minutes after a single injection of PGE2 or synthetic LH-RH, the anterior pituitary were excised for an electromicroscopic study. Ten minute after PGE2, an enlarged Golgi apparatus, well-developed rough endoplasmic reticulum, and decreased secretory granules were observed in pituitary gonadotropins as compared with the control group. These changes were more conspicuous in the pituitaries obtained 30 minutes after PGE2. In Experiment 3, plasma LH levels were determined serially before and 10, 30, 60, and 90 minutes after a single injection of PGE2, LH-RH, or saline. There is a striking resemblance between the PGE2- and LH-RH-treated groups. Plasma LH levels in both these groups reached a peak at 10 minutes and started to decline 30 minutes after PGE2 or LH-RH administration. Our findings would indicate that PGE2 stimulates LH-RH secretion from the hypothalamus.", "contents": "Changes in LH-releasing hormone content of the hypothalamus and electron microscopy of the anterior pituitary after prostaglandin E2 injection in rats. The purpose of this study is to present evidence that prostaglandin (PG) E2 stimulates a release of luteinizing hormone-releasing hormone (LH-RH) from the hypothalmus. In experiment 1, 72 hours after estrogen and progesterone treatment, a solution of 700 mug of PGE2 was injected into the jugular vein. Blood samples for plasma LH and the hypothalamus for LH-RH assay were collected before and 5, 10, and 30 minutes after PGE2 injection and subjected to radioimmunoassay. The total LH-RH content of the hypothalamus decreased from 20.4 to 15.3 ng. at 5 minutes after PGE2 and had returned to the pretreatment level by 30 minutes after PGE2 administration. On the other hand, plasma LH levels increased at 5 minutes, reached a peak at 10 minutes, followed by a decline at 30 minutes after PGE2 injection. In Experiment 2, 10 and 30 minutes after a single injection of PGE2 or synthetic LH-RH, the anterior pituitary were excised for an electromicroscopic study. Ten minute after PGE2, an enlarged Golgi apparatus, well-developed rough endoplasmic reticulum, and decreased secretory granules were observed in pituitary gonadotropins as compared with the control group. These changes were more conspicuous in the pituitaries obtained 30 minutes after PGE2. In Experiment 3, plasma LH levels were determined serially before and 10, 30, 60, and 90 minutes after a single injection of PGE2, LH-RH, or saline. There is a striking resemblance between the PGE2- and LH-RH-treated groups. Plasma LH levels in both these groups reached a peak at 10 minutes and started to decline 30 minutes after PGE2 or LH-RH administration. Our findings would indicate that PGE2 stimulates LH-RH secretion from the hypothalamus."}
{"id": "PMID:1096616", "title": "Bacterial growth inhibition by amniotic fluid. III. Demonstration of the variability of bacterial growth inhibition by amniotic fluid with a new plate-count technique.", "content": "The ability of 50 human amniotic fluid samples to inhibit the growth of Escherichia coli was measured with a new semimicro plate-count technique. A range from bacterial growth-sustaining fluid to bactericidal fluid was observed. When the inhibitory capacities of the fluid samples were correlated with gestational age, a progressive increase was obtained with maximum inhibitory capacity at 36 to 40 weeks. All fluid samples obtained before 20 weeks' gestation were capable of supporting bacterial growth. Fluid samples of 36 to 40 weeks' gestation were all bactericidal or bacteriostatic. More variability in inhibitory capacity was seen after 40 weeks, with an over-all loss of inhibition observed.", "contents": "Bacterial growth inhibition by amniotic fluid. III. Demonstration of the variability of bacterial growth inhibition by amniotic fluid with a new plate-count technique. The ability of 50 human amniotic fluid samples to inhibit the growth of Escherichia coli was measured with a new semimicro plate-count technique. A range from bacterial growth-sustaining fluid to bactericidal fluid was observed. When the inhibitory capacities of the fluid samples were correlated with gestational age, a progressive increase was obtained with maximum inhibitory capacity at 36 to 40 weeks. All fluid samples obtained before 20 weeks' gestation were capable of supporting bacterial growth. Fluid samples of 36 to 40 weeks' gestation were all bactericidal or bacteriostatic. More variability in inhibitory capacity was seen after 40 weeks, with an over-all loss of inhibition observed."}
{"id": "PMID:1096617", "title": "Bacterial growth inhibition by amniotic fluid. IV. Studies on the nature of bacterial inhibition with the use of plate-count determinations.", "content": "Bacterial growth inhibition in amniotic fluid is associated with a compound or class of compounds which resembles antibacterial cationic peptides. The inhibitor studied in amniotic fluid is sensitive to treatment with monobasic and dibasic potassium phosphate, and the inhibitory activity of amniotic fluid is lost following adsorption onto bentomite. The inhibitory component in amniotic fluid interacts with bacterial cells by adsorption or is internalized by viable cells only. The inhibitory quality of amniotic fluid is not destroyed by heating to 100 degrees C. for 15 minutes, and this heat-stable inhibitory activity is associated with the compound or compounds which are phosphate sensitive. A possible mode of antibacterial action which is consistent with the data presented is discussed.", "contents": "Bacterial growth inhibition by amniotic fluid. IV. Studies on the nature of bacterial inhibition with the use of plate-count determinations. Bacterial growth inhibition in amniotic fluid is associated with a compound or class of compounds which resembles antibacterial cationic peptides. The inhibitor studied in amniotic fluid is sensitive to treatment with monobasic and dibasic potassium phosphate, and the inhibitory activity of amniotic fluid is lost following adsorption onto bentomite. The inhibitory component in amniotic fluid interacts with bacterial cells by adsorption or is internalized by viable cells only. The inhibitory quality of amniotic fluid is not destroyed by heating to 100 degrees C. for 15 minutes, and this heat-stable inhibitory activity is associated with the compound or compounds which are phosphate sensitive. A possible mode of antibacterial action which is consistent with the data presented is discussed."}
{"id": "PMID:1096618", "title": "The influence of advancing gestation on group B streptococcal colonization in pregnant women.", "content": "An investigation to determine the throat and vaginal colonization rates of group B streptococci among second- and third-trimester pregnant women was performed. Group B streptococci were recovered from 25.4 per cent of third-trimester and 14.8 per cent of second-trimester parturient (p = less than 0.025). Serotype distribution of isolates was similar among these two study groups. No significant differences in colonization rates were noted on the basis of age, race, parity, or complications of pregnancy.However, postpartum fever occurred in 22 per cent of colonized and only 4 per cent of noncolonized second-trimester parturients. The reason for this significant increase in group B streptococcal colonization rates with advancing gestation remains speculative.", "contents": "The influence of advancing gestation on group B streptococcal colonization in pregnant women. An investigation to determine the throat and vaginal colonization rates of group B streptococci among second- and third-trimester pregnant women was performed. Group B streptococci were recovered from 25.4 per cent of third-trimester and 14.8 per cent of second-trimester parturient (p = less than 0.025). Serotype distribution of isolates was similar among these two study groups. No significant differences in colonization rates were noted on the basis of age, race, parity, or complications of pregnancy.However, postpartum fever occurred in 22 per cent of colonized and only 4 per cent of noncolonized second-trimester parturients. The reason for this significant increase in group B streptococcal colonization rates with advancing gestation remains speculative."}
{"id": "PMID:1096622", "title": "Principles in the management of oculomycosis. XXXI Edward Jackson memorial lecture.", "content": "Effective antifungal therapy must be long-term, nondamaging, penetrating to the eye, and highly active against each patient's fungus. Results of antifungal sensitivity testing of 61 collected ocular fungal pathogens and observations in 25 cases treated with one of the nonpolyene antifungal drugs indicated that infection was rapidly controlled and eradicated with restoration of visual acuity, determined by the degree of disorganization present at the time of commencement of rational specific antifungal therapy. Pimaricin has the widest spectrum, a medium level of activity, and rather poor penetration but is recommended as an antifungal prophylactic and as first-line-therapy for ocular fungal disease while awaiting identification and sensitivity testing of the fungus. Flucytosine combined with amphotericin B, or possibly with clotrimazole or miconazole, is recommended for Candida infections. Clotrimazole is the drug of choice for Aspergillus species although miconazole and econazole are more effective with some isolates. Miconazole and econazole are recommended for miscellaneous filamentous fungi although clotrimazole or thiabendazole are superior in some cases. Each of these drugs may be useful in patients infected with Fusarium who do not respond to primaricin. In these cases, drug use should be guided by the results of antifungal sensitivity testing. In addition to medical antifungal therapy some eyes may require excisional keratoplasty with the lens removal and evacuation of the posterior chamber and anterior vitreous cavity.", "contents": "Principles in the management of oculomycosis. XXXI Edward Jackson memorial lecture. Effective antifungal therapy must be long-term, nondamaging, penetrating to the eye, and highly active against each patient's fungus. Results of antifungal sensitivity testing of 61 collected ocular fungal pathogens and observations in 25 cases treated with one of the nonpolyene antifungal drugs indicated that infection was rapidly controlled and eradicated with restoration of visual acuity, determined by the degree of disorganization present at the time of commencement of rational specific antifungal therapy. Pimaricin has the widest spectrum, a medium level of activity, and rather poor penetration but is recommended as an antifungal prophylactic and as first-line-therapy for ocular fungal disease while awaiting identification and sensitivity testing of the fungus. Flucytosine combined with amphotericin B, or possibly with clotrimazole or miconazole, is recommended for Candida infections. Clotrimazole is the drug of choice for Aspergillus species although miconazole and econazole are more effective with some isolates. Miconazole and econazole are recommended for miscellaneous filamentous fungi although clotrimazole or thiabendazole are superior in some cases. Each of these drugs may be useful in patients infected with Fusarium who do not respond to primaricin. In these cases, drug use should be guided by the results of antifungal sensitivity testing. In addition to medical antifungal therapy some eyes may require excisional keratoplasty with the lens removal and evacuation of the posterior chamber and anterior vitreous cavity."}
{"id": "PMID:1096629", "title": "Intermediate-term corneal storage for penetrating keratoplasty.", "content": "Fifty-seven penetrating keratoplasties were performed using human donor corneal material stored in modified Tissue Culture Medium 199 (TC-199). Fifty-six grafts were clear on the first postoperative day and remained clear for at least three weeks. During the follow-up period (average, 3.7 months), only four additional graft failures occurred. The reduced incidence of early graft edema is attributed to prolonged viability of corneal endothelium maintained in modified TC-199 medium prior to keratoplasty.", "contents": "Intermediate-term corneal storage for penetrating keratoplasty. Fifty-seven penetrating keratoplasties were performed using human donor corneal material stored in modified Tissue Culture Medium 199 (TC-199). Fifty-six grafts were clear on the first postoperative day and remained clear for at least three weeks. During the follow-up period (average, 3.7 months), only four additional graft failures occurred. The reduced incidence of early graft edema is attributed to prolonged viability of corneal endothelium maintained in modified TC-199 medium prior to keratoplasty."}
{"id": "PMID:1096630", "title": "Topical tetracycline and rifampicin therapy of endemic trachoma in Tunisia.", "content": "A controlled chemotherapy trial of trachoma was carried out in a Tunisian oasis among schoolchildren with active disease. We compared 1% tetracycline ointment (79 patients) or 1% rifampicin ointment (76 patients) with 5% boric acid ointment (79 patients). Medications were administered twice daily, six days a week, for ten weeks. Slit-lamp examinations by three ophthalmologists were made independently before treatment as well as five, 19, and 39 weeks after treatment. Bacteriologic cultures were taken during treatment as were smears to detect trachoma agent at each clinical examination. Five weeks after treatment, the intensity of conjunctival disease in the tetracycline and rifampicin groups was reduced significantly when compared with boric acid, but at 19 weeks this suppression was found only in the tetracycline group. Ocular bacterial pathogens were eliminated almost entirely in the two antibiotic groups during treatment. The initial prevalence of trachoma (29to 31%) was significantly reduced in the two antibiotic-treated groups at five weeks and 19 weeks after treatment. The prevalence of trachoma was equally low (7%) in all three groups following retreatment with tetracycline. Although both antibiotics were effective, rifampicin offered no advantage over tetracycline in this trial. Recurrent disease in this school-based treatment study probably was due to reinfection from younger siblings at home. While systematic, community-wide, antibiotic treatment programs are not always possible in countries where trachoma is endemic, limited antibiotic therapy programs should be continued in these areas to reduce the intensity and prevalence of trachoma, even though the disease cannot be eradicated.", "contents": "Topical tetracycline and rifampicin therapy of endemic trachoma in Tunisia. A controlled chemotherapy trial of trachoma was carried out in a Tunisian oasis among schoolchildren with active disease. We compared 1% tetracycline ointment (79 patients) or 1% rifampicin ointment (76 patients) with 5% boric acid ointment (79 patients). Medications were administered twice daily, six days a week, for ten weeks. Slit-lamp examinations by three ophthalmologists were made independently before treatment as well as five, 19, and 39 weeks after treatment. Bacteriologic cultures were taken during treatment as were smears to detect trachoma agent at each clinical examination. Five weeks after treatment, the intensity of conjunctival disease in the tetracycline and rifampicin groups was reduced significantly when compared with boric acid, but at 19 weeks this suppression was found only in the tetracycline group. Ocular bacterial pathogens were eliminated almost entirely in the two antibiotic groups during treatment. The initial prevalence of trachoma (29to 31%) was significantly reduced in the two antibiotic-treated groups at five weeks and 19 weeks after treatment. The prevalence of trachoma was equally low (7%) in all three groups following retreatment with tetracycline. Although both antibiotics were effective, rifampicin offered no advantage over tetracycline in this trial. Recurrent disease in this school-based treatment study probably was due to reinfection from younger siblings at home. While systematic, community-wide, antibiotic treatment programs are not always possible in countries where trachoma is endemic, limited antibiotic therapy programs should be continued in these areas to reduce the intensity and prevalence of trachoma, even though the disease cannot be eradicated."}
{"id": "PMID:1096633", "title": "The development and resolution of glomerular basement membrane changes associated with subepithelial immune deposits.", "content": "The morphogenesis of glomerular basement membrane changes associated with subepithelial immune deposits was studied in kidney biopsies from patients with gold-induced membranous glomerulonephoritis. Serial biopsies showed focal accumulations of additional basement membrane material around the deposits, suggesting that the deposited material stimulated the epithelium to increased synthesis. Moreover, the deposits were gradually displaced towards the inner (endothelia) side of the basement membrane during the course of the disease, suggesting that this layer undergoes a slow continuous turnover, with removal at its endothelial aspect. The two processes--increased epithelial synthesis and turnover--are suggested to constitute the basis of a natural healing process resulting in elimination of the deposits and structural restoration of the basement membrane. The epithelial slit membranes were dislocated externally by the deposits or the excessive basement membrane material, indicating that their barrier function is preserved even in this pathologic condition.", "contents": "The development and resolution of glomerular basement membrane changes associated with subepithelial immune deposits. The morphogenesis of glomerular basement membrane changes associated with subepithelial immune deposits was studied in kidney biopsies from patients with gold-induced membranous glomerulonephoritis. Serial biopsies showed focal accumulations of additional basement membrane material around the deposits, suggesting that the deposited material stimulated the epithelium to increased synthesis. Moreover, the deposits were gradually displaced towards the inner (endothelia) side of the basement membrane during the course of the disease, suggesting that this layer undergoes a slow continuous turnover, with removal at its endothelial aspect. The two processes--increased epithelial synthesis and turnover--are suggested to constitute the basis of a natural healing process resulting in elimination of the deposits and structural restoration of the basement membrane. The epithelial slit membranes were dislocated externally by the deposits or the excessive basement membrane material, indicating that their barrier function is preserved even in this pathologic condition."}
{"id": "PMID:1096634", "title": "Immunologic enhancement of rat renal allografts. III. Immunopathologic lesions and rejection in long-surviving passively enhanced grafts.", "content": "Immunologic enhancement of renal allografts from (Lewis times Brown Norway) F1 to Lewis rats was achieved by administering a single dose of antidonor serum at the time of transplantation. A series of grafts functioning for 1 to 4 months after transplantation were examined by light and immunofluorescence microscopy to evaluate the long-term protective effects of the enhancing serum and to determine if previously unobserved lesions appeared in long survivors. Despite the absence of detectable circulating cytotoxic alloantibody, long-term allografts showed necrotizing glomerular and arterial lesions which resembled those seen in acutely rejecting grafts and were compatible with humoral rejection. Thus, in this model, there is a late decline in the ability of passive enhancement to inhibit humoral rejection. Long-term grafts also developed tubular lesions with deposition of immunoglobulin and complement on the tubular basement membranes (TBM). Anti-TBM antibodies were demonstrated in recipients' sera and found to be organ specific but not major histocompatibility antigen or species specific. This tubular lesion is therefore a unique form of allograft injury in which the immune response is directed against tissue antigen(s) which are distinct from the major histocompatibility antigens that induce rejection.", "contents": "Immunologic enhancement of rat renal allografts. III. Immunopathologic lesions and rejection in long-surviving passively enhanced grafts. Immunologic enhancement of renal allografts from (Lewis times Brown Norway) F1 to Lewis rats was achieved by administering a single dose of antidonor serum at the time of transplantation. A series of grafts functioning for 1 to 4 months after transplantation were examined by light and immunofluorescence microscopy to evaluate the long-term protective effects of the enhancing serum and to determine if previously unobserved lesions appeared in long survivors. Despite the absence of detectable circulating cytotoxic alloantibody, long-term allografts showed necrotizing glomerular and arterial lesions which resembled those seen in acutely rejecting grafts and were compatible with humoral rejection. Thus, in this model, there is a late decline in the ability of passive enhancement to inhibit humoral rejection. Long-term grafts also developed tubular lesions with deposition of immunoglobulin and complement on the tubular basement membranes (TBM). Anti-TBM antibodies were demonstrated in recipients' sera and found to be organ specific but not major histocompatibility antigen or species specific. This tubular lesion is therefore a unique form of allograft injury in which the immune response is directed against tissue antigen(s) which are distinct from the major histocompatibility antigens that induce rejection."}
{"id": "PMID:1096636", "title": "Violence at home: A review of the literature.", "content": "Studies on family violence have analyzed the phenomenon from psychological, social, and cultural perspectives. A review of the literature shows that the available evidence is not contradictory, leading to the conclusion that a comprehensive theory of violence at home must take into account factors at these several levels, placing individual functioning within the social group and within the culture norms by which the group operates. A theory of violence at home, and suggestions for further research, are offered.", "contents": "Violence at home: A review of the literature. Studies on family violence have analyzed the phenomenon from psychological, social, and cultural perspectives. A review of the literature shows that the available evidence is not contradictory, leading to the conclusion that a comprehensive theory of violence at home must take into account factors at these several levels, placing individual functioning within the social group and within the culture norms by which the group operates. A theory of violence at home, and suggestions for further research, are offered."}
{"id": "PMID:1096638", "title": "New studies of post-Pleistocene human skeletal remains from the Rift Valley, Kenya.", "content": "Prehistoric human crania from Bromhead's Site, Willey's Kopje, Makalia Burial Site, Nakuru, and other localities in the Eastern Rift Valley of Kenya are reassessed using measurements and a multivariate statistical approach. Materials available for comparison include series of Bushman and Hottentot crania. South and East African Negroes, and Egyptians. Up to 34 cranial measurements taken on these series are utilized to construct three multiple discriminant frameworks, each of which can assign modern individuals to a correct group with considerable accuracy. When the prehistoric crania are classified with the help of these discriminants, results indicate that several of the skulls are best grouped with modern Negroes. This is especially clear in the case of individuals from Bromhead's Site, Willey's Kopje, and Nakuru, and the evidence hardly suggests post-Pleistocene domination of the Rift and surrounding territory by \"Mediterranean\" Caucasoids, as has been claimed. Recent linguistic and archaeological findings are also reviewed, and these seem to support application of the term Nilotic Negro to the early Rift populations.", "contents": "New studies of post-Pleistocene human skeletal remains from the Rift Valley, Kenya. Prehistoric human crania from Bromhead's Site, Willey's Kopje, Makalia Burial Site, Nakuru, and other localities in the Eastern Rift Valley of Kenya are reassessed using measurements and a multivariate statistical approach. Materials available for comparison include series of Bushman and Hottentot crania. South and East African Negroes, and Egyptians. Up to 34 cranial measurements taken on these series are utilized to construct three multiple discriminant frameworks, each of which can assign modern individuals to a correct group with considerable accuracy. When the prehistoric crania are classified with the help of these discriminants, results indicate that several of the skulls are best grouped with modern Negroes. This is especially clear in the case of individuals from Bromhead's Site, Willey's Kopje, and Nakuru, and the evidence hardly suggests post-Pleistocene domination of the Rift and surrounding territory by \"Mediterranean\" Caucasoids, as has been claimed. Recent linguistic and archaeological findings are also reviewed, and these seem to support application of the term Nilotic Negro to the early Rift populations."}
{"id": "PMID:1096639", "title": "Morphometric analysis of Cartesian coordinates of the human skull.", "content": "A method for locating the three dimensional coordinates of cranial landmarks with respect to the Frankfort, midsagittal, and coronal planes is presented. Sliding calipers were used to obtain the distances from left and right porion and apex to each landmark, except for a few points where spreading calipers are required. In the present example, 35 landmarks (for a total of 105 measurements) were located for each of 35 Peruvian precolumbian skulls. These distances were entered into a program (SKULL) which calculates the Cartesian coordinates of each landmark. The XYZ coordinates of each landmark contain all the information necessary for calculation of the distances between any two landmarks, and these distances may also be obtained as output from program SKULL, if desired (595 distances if all 35 landmarks are used). Reliability of the location of coordinates was determined by comparing computed distances among selected landmarks from program SKULL with traditional anthropometric measurements. Satisfactory agreements were found. Direct multivariate analysis of the coordinates of the landmarks produced insights not available in traditional multivariate analysis of conventional anthropometric measurements.", "contents": "Morphometric analysis of Cartesian coordinates of the human skull. A method for locating the three dimensional coordinates of cranial landmarks with respect to the Frankfort, midsagittal, and coronal planes is presented. Sliding calipers were used to obtain the distances from left and right porion and apex to each landmark, except for a few points where spreading calipers are required. In the present example, 35 landmarks (for a total of 105 measurements) were located for each of 35 Peruvian precolumbian skulls. These distances were entered into a program (SKULL) which calculates the Cartesian coordinates of each landmark. The XYZ coordinates of each landmark contain all the information necessary for calculation of the distances between any two landmarks, and these distances may also be obtained as output from program SKULL, if desired (595 distances if all 35 landmarks are used). Reliability of the location of coordinates was determined by comparing computed distances among selected landmarks from program SKULL with traditional anthropometric measurements. Satisfactory agreements were found. Direct multivariate analysis of the coordinates of the landmarks produced insights not available in traditional multivariate analysis of conventional anthropometric measurements."}
{"id": "PMID:1096640", "title": "Cranial dysraphism mistaken for trephination.", "content": "Attention is called to a disputed diagnosis of trephination reported for a perforated skull of a prehistoric New England Indian. The perforation, surrounded by a saucer-like depression, is located exactly in the midline just in front of bregma. The finding of a similar lesion in a prehistoric Indian skull from the North Coast of Peru--where, as in New England, good evidence of the practice of trephination is lacking--provides support for a more reasonable diagnosis for both cases: congenital cranial dysraphism, specifically encephalocele. Information about dysraphic states from modern clinical experience is summarized.", "contents": "Cranial dysraphism mistaken for trephination. Attention is called to a disputed diagnosis of trephination reported for a perforated skull of a prehistoric New England Indian. The perforation, surrounded by a saucer-like depression, is located exactly in the midline just in front of bregma. The finding of a similar lesion in a prehistoric Indian skull from the North Coast of Peru--where, as in New England, good evidence of the practice of trephination is lacking--provides support for a more reasonable diagnosis for both cases: congenital cranial dysraphism, specifically encephalocele. Information about dysraphic states from modern clinical experience is summarized."}
{"id": "PMID:1096641", "title": "Pressure-flow relations in the vessels of the canine aortic arch.", "content": "Simultaneous measurements of pressure and flow have been made in the vessels of the aortic arch in chloralose-anesthetized dogs under three conditions; control, cardiac sympathetic (SS), and vagal nerve stimulation (VS). The specific sites studied include ascending aorta (AA), descending thoracic aorta (DTA), and brachiocephalic (BCA), left subclavian (LSC), and common carotid arteries (CA). Recorded data were analyzed by Fourier series methods and were used to calculate vascular impedance spectra, pressure and flow propagation characteristics, and hydraulic power. The distribution of cardiac output was found to be 70% to DTA, 22.2% to BCA, and 6.5% to LSC. The fraction of cardiac output to the BCA, LSC, and CA was increased by both SS and VS. The hydraulic power was distributed to the arch vessels nearly the same as the cardiac output. Hydraulic power dissipated in the arch vessels averaged 6% of the total AA hydraulic power and was increased by SS and decreased by VS. The results suggest that viscous losses in the arterial walls represent a considerable portion of the power dissipation. A similarity found in the vascular impedance spectra at the various sites for all three conditions was also observed for the transmission characteristics of pressure and flow harmonics between the sites. The results suggest that major reflections do not occur in the proximal aorta.", "contents": "Pressure-flow relations in the vessels of the canine aortic arch. Simultaneous measurements of pressure and flow have been made in the vessels of the aortic arch in chloralose-anesthetized dogs under three conditions; control, cardiac sympathetic (SS), and vagal nerve stimulation (VS). The specific sites studied include ascending aorta (AA), descending thoracic aorta (DTA), and brachiocephalic (BCA), left subclavian (LSC), and common carotid arteries (CA). Recorded data were analyzed by Fourier series methods and were used to calculate vascular impedance spectra, pressure and flow propagation characteristics, and hydraulic power. The distribution of cardiac output was found to be 70% to DTA, 22.2% to BCA, and 6.5% to LSC. The fraction of cardiac output to the BCA, LSC, and CA was increased by both SS and VS. The hydraulic power was distributed to the arch vessels nearly the same as the cardiac output. Hydraulic power dissipated in the arch vessels averaged 6% of the total AA hydraulic power and was increased by SS and decreased by VS. The results suggest that viscous losses in the arterial walls represent a considerable portion of the power dissipation. A similarity found in the vascular impedance spectra at the various sites for all three conditions was also observed for the transmission characteristics of pressure and flow harmonics between the sites. The results suggest that major reflections do not occur in the proximal aorta."}
{"id": "PMID:1096642", "title": "Hormone-substrate responses to total fasting in lean and obese mice.", "content": "The hormone-substrate milieu has been investigated in male fasted lean (C57BL/6-+/+) mice and mutant obese mice of the same strain (C57BL/6-obob). The lean mouse, in winter, mobilized insufficient fat (due to inadequate stores) to permit survival beyong 3 days and was unable to achieve any degree of conservation of vital protein stores. By contrast, in summer, the same animals survived 7 days and showed evidence of greater and more sustained fat mobilization and ketosis and the ability to conserve protein. The insulin, glucagon, and insulin/glucagon molar ratios changed in both groups in a direction consistent with conversion to a catabolic state, and hence were probably largely responsible for the mobilization of substrates and stimulation of gluconeogenesis and ketogenesis. The seasonal difference in response is unexplained. The obob mice, generally employed as a model for obesity, hyperglycemia, and hyperinsulinemia showed these features but also adapted to fasting in a fashion permitting prolonged survival during this state. In a fashion analogous to that known to occur in man, these animals developed fall in glycemia, rise in circulating fat-derived substrates, and marked protein conservation. Profound fall in insulinemia was associated with a fall in glucagonemia, the latter from normal levels. Thus the initial markedly \"anabolic\" insulin/glucagon molar ratio diminished, but nevertheless remained higher than at any time in the lean mice. Pancreatic contents of insulin showed markedly different changes with fasting in obob compared with lean mice. The ability of the obese mouse to adapt to prolonged fasting in a fashion largely analogous to that of man renders it a useful model for the study of metabolism in this state, with the potential of applicability of findings to man.", "contents": "Hormone-substrate responses to total fasting in lean and obese mice. The hormone-substrate milieu has been investigated in male fasted lean (C57BL/6-+/+) mice and mutant obese mice of the same strain (C57BL/6-obob). The lean mouse, in winter, mobilized insufficient fat (due to inadequate stores) to permit survival beyong 3 days and was unable to achieve any degree of conservation of vital protein stores. By contrast, in summer, the same animals survived 7 days and showed evidence of greater and more sustained fat mobilization and ketosis and the ability to conserve protein. The insulin, glucagon, and insulin/glucagon molar ratios changed in both groups in a direction consistent with conversion to a catabolic state, and hence were probably largely responsible for the mobilization of substrates and stimulation of gluconeogenesis and ketogenesis. The seasonal difference in response is unexplained. The obob mice, generally employed as a model for obesity, hyperglycemia, and hyperinsulinemia showed these features but also adapted to fasting in a fashion permitting prolonged survival during this state. In a fashion analogous to that known to occur in man, these animals developed fall in glycemia, rise in circulating fat-derived substrates, and marked protein conservation. Profound fall in insulinemia was associated with a fall in glucagonemia, the latter from normal levels. Thus the initial markedly \"anabolic\" insulin/glucagon molar ratio diminished, but nevertheless remained higher than at any time in the lean mice. Pancreatic contents of insulin showed markedly different changes with fasting in obob compared with lean mice. The ability of the obese mouse to adapt to prolonged fasting in a fashion largely analogous to that of man renders it a useful model for the study of metabolism in this state, with the potential of applicability of findings to man."}
{"id": "PMID:1096643", "title": "Separate lateral hypothalamic pathways for extracellular and intracellular thirst.", "content": "Small lesions of the midlateral zone of the lateral hypothalamus in rats attentuated water intake elicted by the central microinjection angiotensin or by the peripheral injection of isoproterenol or renin without attenuating drinking to peripherally administered hypertonic saline. Lesions placed further lateral in the hypothalamus, which destroyed the medial aspects of the internal capsule and globus pallidus, produced a marked decrease in water intake induced by  hypertonic saline. Abaltion of the ventromedial nucleus of the hypothalamus increased drinking elicited by angiotension, isoproterenol, or renin. These  results suggest that extracellular and intracellular thirst stimuli are mediated by separate neural pathways at the level of the lateral hypothalamus.", "contents": "Separate lateral hypothalamic pathways for extracellular and intracellular thirst. Small lesions of the midlateral zone of the lateral hypothalamus in rats attentuated water intake elicted by the central microinjection angiotensin or by the peripheral injection of isoproterenol or renin without attenuating drinking to peripherally administered hypertonic saline. Lesions placed further lateral in the hypothalamus, which destroyed the medial aspects of the internal capsule and globus pallidus, produced a marked decrease in water intake induced by  hypertonic saline. Abaltion of the ventromedial nucleus of the hypothalamus increased drinking elicited by angiotension, isoproterenol, or renin. These  results suggest that extracellular and intracellular thirst stimuli are mediated by separate neural pathways at the level of the lateral hypothalamus."}
{"id": "PMID:1096644", "title": "Permeability limitation in estimation of local brain blood flow with [14C]antipyrine.", "content": "Influence of capillary permeability on local cerebral blood flow (LCBF) estimated by the autoradiograhpic diffusible-indicator method was analyzed by computer simulation. Its influence increases with increasing flow. With normal perfusion rates in gray matter, capillary permeability coefficient x surface area (PS value) must exceed 0.12 cm3-s(-1)-g(-1) for estimated LCBF to attain 95% of true values in all structures. In white matter, with its lower perfusion rates, the PS value must exceed 0.035 cm3-s(-1)-g(-1) for equivalent accuracy. [14C]antipyrine, widely used in the LCBF technique, has a PS value below these values and leads to undersestimation of LCBF. Experimental studies in cats in which LCBF was measured with either [14C]antipyrine or the freely diffusible, radioactive gas, [131I]trifluoroiodomethane, demonstrated consistently lower estimates of LCBF with [14C]antipyrine than with the gas; the deviations in the various cerebral structures approximated those predicted by the theoretical analyses. These results suggest that the uptake of [14C]antipyrine by cerebral tissues is diffusion limited as well as flow limited, and it therefore is not an ideal tracer for the autoradiographic LCBF technique.", "contents": "Permeability limitation in estimation of local brain blood flow with [14C]antipyrine. Influence of capillary permeability on local cerebral blood flow (LCBF) estimated by the autoradiograhpic diffusible-indicator method was analyzed by computer simulation. Its influence increases with increasing flow. With normal perfusion rates in gray matter, capillary permeability coefficient x surface area (PS value) must exceed 0.12 cm3-s(-1)-g(-1) for estimated LCBF to attain 95% of true values in all structures. In white matter, with its lower perfusion rates, the PS value must exceed 0.035 cm3-s(-1)-g(-1) for equivalent accuracy. [14C]antipyrine, widely used in the LCBF technique, has a PS value below these values and leads to undersestimation of LCBF. Experimental studies in cats in which LCBF was measured with either [14C]antipyrine or the freely diffusible, radioactive gas, [131I]trifluoroiodomethane, demonstrated consistently lower estimates of LCBF with [14C]antipyrine than with the gas; the deviations in the various cerebral structures approximated those predicted by the theoretical analyses. These results suggest that the uptake of [14C]antipyrine by cerebral tissues is diffusion limited as well as flow limited, and it therefore is not an ideal tracer for the autoradiographic LCBF technique."}
{"id": "PMID:1096645", "title": "Caffeine versus methylphenidate and d-amphetamine in minimal brain dysfunction: a double-blind comparison.", "content": "The authors compared the efficacy of caffeine, methylphenidate, and d-amphetamine in children with minimal brain dysfunction using a double-blind crossover design. The slight improvement with caffeine was not significantly better than placebo. Both prescription drugs resulted in significant improvement and were significantly superior to caffeine. The authors suggest that the discrepancy between these results and an earlier, more optimistic report mat stem from the use in this study of pure caffeine rather than whole coffee.", "contents": "Caffeine versus methylphenidate and d-amphetamine in minimal brain dysfunction: a double-blind comparison. The authors compared the efficacy of caffeine, methylphenidate, and d-amphetamine in children with minimal brain dysfunction using a double-blind crossover design. The slight improvement with caffeine was not significantly better than placebo. Both prescription drugs resulted in significant improvement and were significantly superior to caffeine. The authors suggest that the discrepancy between these results and an earlier, more optimistic report mat stem from the use in this study of pure caffeine rather than whole coffee."}
{"id": "PMID:1096647", "title": "Psychotherapy in India: past, present, and future.", "content": "If psychotherapy is defined as \"interpersonal method of mitigating suffering\" then many psychotherapeutic systems have existed in India a long time. Unlike Western systems, these have lacked a clinical bias but have provided a more global framework. The future of psychotherapy in both East and West lies in discovering a conceptual framework with universal validity within which ad hoc therapies--for symptom relief, personality development, or interpersonal adjustment--can be developed.", "contents": "Psychotherapy in India: past, present, and future. If psychotherapy is defined as \"interpersonal method of mitigating suffering\" then many psychotherapeutic systems have existed in India a long time. Unlike Western systems, these have lacked a clinical bias but have provided a more global framework. The future of psychotherapy in both East and West lies in discovering a conceptual framework with universal validity within which ad hoc therapies--for symptom relief, personality development, or interpersonal adjustment--can be developed."}
{"id": "PMID:1096651", "title": "Solitary phalangeal defect in the hand. Report of 2 rare cases.", "content": "Two unusual causes of bone defect in the finger are described. In one patient, erosion of the proximal phalanx was caused by a ring that eroded its way through the soft tissue and bone after an injury. In the other, a subungual wart caused a defect on the distal phalanx.", "contents": "Solitary phalangeal defect in the hand. Report of 2 rare cases. Two unusual causes of bone defect in the finger are described. In one patient, erosion of the proximal phalanx was caused by a ring that eroded its way through the soft tissue and bone after an injury. In the other, a subungual wart caused a defect on the distal phalanx."}
{"id": "PMID:1096653", "title": "The circle system without absorber. Use with controlled ventilation.", "content": "An investigation is described into the performance of two types of circle system used without a carbon dioxide absorber in both the laboratory and in the operating theatre with controlled ventilation. The newer system was found to be more efficient than the old but in both types of system the tidal volume was found to be very important, a large tidal volume resulted in increased efficiency. The possibility of predicting arterial blood carbon dioxide using this system is briefly discussed, and rules are suggested for the use of the system.", "contents": "The circle system without absorber. Use with controlled ventilation. An investigation is described into the performance of two types of circle system used without a carbon dioxide absorber in both the laboratory and in the operating theatre with controlled ventilation. The newer system was found to be more efficient than the old but in both types of system the tidal volume was found to be very important, a large tidal volume resulted in increased efficiency. The possibility of predicting arterial blood carbon dioxide using this system is briefly discussed, and rules are suggested for the use of the system."}
{"id": "PMID:1096654", "title": "The Retec breathing assistor. An application of fluidics to apparatus for inhalation therapy.", "content": "The Retec breathing assistor, with its gluidic valve, is an interesting method of providing assisted positive pressure inspiration, a resistive load to expiration and a capability for the administration of drugs by nebuliser. It requires some co-operation but very little training by patients, and is easily cleaned and maintained.", "contents": "The Retec breathing assistor. An application of fluidics to apparatus for inhalation therapy. The Retec breathing assistor, with its gluidic valve, is an interesting method of providing assisted positive pressure inspiration, a resistive load to expiration and a capability for the administration of drugs by nebuliser. It requires some co-operation but very little training by patients, and is easily cleaned and maintained."}
{"id": "PMID:1096655", "title": "A portable self-inflator-nebuliser.", "content": "An inexpensive portable apparatus for the administration of topical nebulisation therapy is described.", "contents": "A portable self-inflator-nebuliser. An inexpensive portable apparatus for the administration of topical nebulisation therapy is described."}
{"id": "PMID:1096656", "title": "The immersion incident.", "content": "The ever increasing participation in aquatic recreational activities is a major factor in the increasing number of deaths due to accidental immersion. Some of these deaths occur while undergoing resuscitative efforts immediately following rescue, on admission to hospital, or even up to 19 days after the immersion incident. Drowning, either acute or its delayed effects, is chiefly responsible for these deaths, but in a number, hypothermia occurring alone or complicating drowning, is the likely explanation. This paper examines the problem and proposes a regime of management.", "contents": "The immersion incident. The ever increasing participation in aquatic recreational activities is a major factor in the increasing number of deaths due to accidental immersion. Some of these deaths occur while undergoing resuscitative efforts immediately following rescue, on admission to hospital, or even up to 19 days after the immersion incident. Drowning, either acute or its delayed effects, is chiefly responsible for these deaths, but in a number, hypothermia occurring alone or complicating drowning, is the likely explanation. This paper examines the problem and proposes a regime of management."}
{"id": "PMID:1096664", "title": "Some eighteenth century authorities on the resuscitation of the apparently drowned.", "content": "Attempts to resuscitate the apparently drowned began seriously in the eighteenth century. The theories and methods of resuscitation used or advocated by some of the early European workers have been reviewed. Many of these, including artificial ventilation, chest compression, endotracheal intubation and electrical stimulation of the heart seem to have been forgotten during the nineteenth century and not used again until recent times.", "contents": "Some eighteenth century authorities on the resuscitation of the apparently drowned. Attempts to resuscitate the apparently drowned began seriously in the eighteenth century. The theories and methods of resuscitation used or advocated by some of the early European workers have been reviewed. Many of these, including artificial ventilation, chest compression, endotracheal intubation and electrical stimulation of the heart seem to have been forgotten during the nineteenth century and not used again until recent times."}
{"id": "PMID:1096665", "title": "Arterial oxygen tensions during the induction of ether and air anaesthesia with spontaneous respiration.", "content": "Arterial oxygen tensions were measured during the induction of ether and air anaesthesia with the EMO Inhaler and spontaneous respiration. Severe degrees of anoxia were demonstrated; these were often associated with normal or above normal minute volumes. The danger of anoxia can be alleviated either by supplying oxygen or by the use of relaxants and positive pressure ventilation or both. A choice of priorities has often to be made by those in authority in developing countries where resources of material and manpower are limited and logistic support erratic. Every effort should be made to encourage a supply of oxygen in such circumstances. The training of anaesthetic staff is of paramount importance and it is a great pity that the equivocal attitude of the professions, both medical and nursing, in Britain towards the training of nurses and paramedical personnel in anaesthesia does little to encourage such training in developing countries.", "contents": "Arterial oxygen tensions during the induction of ether and air anaesthesia with spontaneous respiration. Arterial oxygen tensions were measured during the induction of ether and air anaesthesia with the EMO Inhaler and spontaneous respiration. Severe degrees of anoxia were demonstrated; these were often associated with normal or above normal minute volumes. The danger of anoxia can be alleviated either by supplying oxygen or by the use of relaxants and positive pressure ventilation or both. A choice of priorities has often to be made by those in authority in developing countries where resources of material and manpower are limited and logistic support erratic. Every effort should be made to encourage a supply of oxygen in such circumstances. The training of anaesthetic staff is of paramount importance and it is a great pity that the equivocal attitude of the professions, both medical and nursing, in Britain towards the training of nurses and paramedical personnel in anaesthesia does little to encourage such training in developing countries."}
{"id": "PMID:1096666", "title": "Hyoscine butylbromide: a useful premedicant.", "content": "The actions of intravenous hyoscine butylbromide (Buscopan) and atropine sulphate on reduction of upper respiratory tract secretions during anaesthesia are compared. Hyoscine butylbromide has a significantly more powerful action.", "contents": "Hyoscine butylbromide: a useful premedicant. The actions of intravenous hyoscine butylbromide (Buscopan) and atropine sulphate on reduction of upper respiratory tract secretions during anaesthesia are compared. Hyoscine butylbromide has a significantly more powerful action."}
{"id": "PMID:1096667", "title": "[Glaucoma, tonometry and anaesthesia (author's transl)].", "content": "Definition and classification of glaucomas. Indication of drugs contraindicated in glaucomas. Discussion of the problem of tonometry in anaesthesia in congenital glaucoma suspicion with special regard to factors influencing intraocular pressure. Discussion of correlation between intraocular pressure and systemic blood pressure. Report on own experiences with Ketamine and discussion of advantage and disadvantage of the drug for tonometry in anaesthesia in infants. Explanation of the method of impression--and applanation tonometry with regard to Glaucotest, a new borderline--tonometer suitable for the anaesthesiologist.", "contents": "[Glaucoma, tonometry and anaesthesia (author's transl)]. Definition and classification of glaucomas. Indication of drugs contraindicated in glaucomas. Discussion of the problem of tonometry in anaesthesia in congenital glaucoma suspicion with special regard to factors influencing intraocular pressure. Discussion of correlation between intraocular pressure and systemic blood pressure. Report on own experiences with Ketamine and discussion of advantage and disadvantage of the drug for tonometry in anaesthesia in infants. Explanation of the method of impression--and applanation tonometry with regard to Glaucotest, a new borderline--tonometer suitable for the anaesthesiologist."}
{"id": "PMID:1096675", "title": "Differentiation of sympathicoblasts in cultures of chick ganglia: light and electron microscopic, fluorescence and enzyme histochemical observations.", "content": "Immature sympathetic ganglia prepared from 5 1/2-or 6-day-old chick embryos were cultured up to one month. The in vitro development was followed by phase microscopy, electron microscopy and using histochemistry for catecholamines, monoamine oxidase and cholinesterases. During the first week of culture extensive plexuses of nerve fibres were formed between and around the clusters of nerve cells. Mature-looking neurons were observed in the cultures by phase microscopy after three weeks, at which age the mean diameter of the perikarya was more than doubled. Varying catecholamine fluorescence was observed in the perikarya during the entire culture period. The nerve fibres showed usually only weak fluorescence, but, in the older cultures, bright varicosities were regularly found in the fibres. Monoamine oxidase activity was demonstrated already at three days of culture and the reaction was maintained positive. Weak or moderate acetyl-cholinesterase activity was demonstrated in the sympathicoblasts and young sympathetic neurons and their processes. The axolemma showed acetylcholinesterase activity also around the nerve terminals containing small dense cored vesicles. Reactions for the non-specific cholinesterases were negative. Electron microscopy of the 30-day-old cultures revealed that the clusters of nerve cells consisted of mature sympathetic neurons, which contained large (60-200 nm) and small (35-60 nm) granular catecholamine-storing vesicles. Glial cells were almost totally lacking. Large numbers of nerve terminals containing both large and small granular vesicles were observed in the clusters, often in synaptic contact with the sympathetic neurons. It is concluded that the primitive sympathicoblasts are, in favourable conditions, capable of differentiation in culture up to mature sympathetic neurons.", "contents": "Differentiation of sympathicoblasts in cultures of chick ganglia: light and electron microscopic, fluorescence and enzyme histochemical observations. Immature sympathetic ganglia prepared from 5 1/2-or 6-day-old chick embryos were cultured up to one month. The in vitro development was followed by phase microscopy, electron microscopy and using histochemistry for catecholamines, monoamine oxidase and cholinesterases. During the first week of culture extensive plexuses of nerve fibres were formed between and around the clusters of nerve cells. Mature-looking neurons were observed in the cultures by phase microscopy after three weeks, at which age the mean diameter of the perikarya was more than doubled. Varying catecholamine fluorescence was observed in the perikarya during the entire culture period. The nerve fibres showed usually only weak fluorescence, but, in the older cultures, bright varicosities were regularly found in the fibres. Monoamine oxidase activity was demonstrated already at three days of culture and the reaction was maintained positive. Weak or moderate acetyl-cholinesterase activity was demonstrated in the sympathicoblasts and young sympathetic neurons and their processes. The axolemma showed acetylcholinesterase activity also around the nerve terminals containing small dense cored vesicles. Reactions for the non-specific cholinesterases were negative. Electron microscopy of the 30-day-old cultures revealed that the clusters of nerve cells consisted of mature sympathetic neurons, which contained large (60-200 nm) and small (35-60 nm) granular catecholamine-storing vesicles. Glial cells were almost totally lacking. Large numbers of nerve terminals containing both large and small granular vesicles were observed in the clusters, often in synaptic contact with the sympathetic neurons. It is concluded that the primitive sympathicoblasts are, in favourable conditions, capable of differentiation in culture up to mature sympathetic neurons."}
{"id": "PMID:1096676", "title": "Electron microscopic localization of calcium in vascular smooth muscle.", "content": "Potassium pyroantimonate has been employed in this study to localize calcium in the vascular smooth muscle of the thoracic aorta of the rabbit. The pyroantimonate ion precipitates sodium, magnesium and calcium. Incubation of theisolated thoracic aorta in a high potassium bathing medium which does not contain sodium, magnesium or calcium depletes the tissue of sodium. Addition of 10.8 mM CaC12 to the incubation medium results in well-localized depositions of reaction product, presumably that of calcium pyroantimonate, in mitochondria, sarcoplasmic reticulum, and at the plasma membrane. Some or all of these organelles may, therefore, play a vital role in the contraction-relaxation cycle of vascular smooth muscle.", "contents": "Electron microscopic localization of calcium in vascular smooth muscle. Potassium pyroantimonate has been employed in this study to localize calcium in the vascular smooth muscle of the thoracic aorta of the rabbit. The pyroantimonate ion precipitates sodium, magnesium and calcium. Incubation of theisolated thoracic aorta in a high potassium bathing medium which does not contain sodium, magnesium or calcium depletes the tissue of sodium. Addition of 10.8 mM CaC12 to the incubation medium results in well-localized depositions of reaction product, presumably that of calcium pyroantimonate, in mitochondria, sarcoplasmic reticulum, and at the plasma membrane. Some or all of these organelles may, therefore, play a vital role in the contraction-relaxation cycle of vascular smooth muscle."}
{"id": "PMID:1096677", "title": "New advances in pulmonary gas exchange.", "content": "Unevenness of ventilation and blood flow within the lung is of great clinical importance because this mechanism is the commonest cause of hypoxemia and respiratory failure. In spite of this, little is known about the distribution of ventilation-perfusion ratios in disease. A new technic for determining these distributions is described and examples are given. An important finding is instability of areas with low ventilation-perfusion ratios during oxygen breathing.", "contents": "New advances in pulmonary gas exchange. Unevenness of ventilation and blood flow within the lung is of great clinical importance because this mechanism is the commonest cause of hypoxemia and respiratory failure. In spite of this, little is known about the distribution of ventilation-perfusion ratios in disease. A new technic for determining these distributions is described and examples are given. An important finding is instability of areas with low ventilation-perfusion ratios during oxygen breathing."}
{"id": "PMID:1096679", "title": "Effects of intraoperative PEEP on postoperative arterial oxygenation.", "content": "Previous studies have suggested that early postoperative hypozemia may be due to a reduction of functional residual capacity (FRC) during anesthesia and surgery. Positive end-expiratory pressure (PEEP) has been recommended as a means of maintaining FRC and improving arterial oxygenation. Fifteen selected patients undergoing peripheral surgical procedures were anesthetized with one of three types of ventilatory patterns: (1) spontaneous respiration; (2) controlled ventilation; and (3) controlled ventilation with 10 cm. of water PEEP. Pao2 were sampled at selected preoperative, intraoperative, and postoperative periods. Patients receiving PEEP maintained the highest mean Pao2 intraoperatively. However, the beneficial effect of intraoperative PEEP was not sustained in the recovery room, where all patients experienced the same degree of arterial hypoxemia.", "contents": "Effects of intraoperative PEEP on postoperative arterial oxygenation. Previous studies have suggested that early postoperative hypozemia may be due to a reduction of functional residual capacity (FRC) during anesthesia and surgery. Positive end-expiratory pressure (PEEP) has been recommended as a means of maintaining FRC and improving arterial oxygenation. Fifteen selected patients undergoing peripheral surgical procedures were anesthetized with one of three types of ventilatory patterns: (1) spontaneous respiration; (2) controlled ventilation; and (3) controlled ventilation with 10 cm. of water PEEP. Pao2 were sampled at selected preoperative, intraoperative, and postoperative periods. Patients receiving PEEP maintained the highest mean Pao2 intraoperatively. However, the beneficial effect of intraoperative PEEP was not sustained in the recovery room, where all patients experienced the same degree of arterial hypoxemia."}
{"id": "PMID:1096685", "title": "Antazoline phosphate and naphazoline hydrochloride, singly and in combination for the treatment of allergic conjunctivitis-a controlled, double-blind clinical trial.", "content": "A controlled, double-blind comparison of naphalzoline hydrochloride 0.05%, antazoline phosphate 0.5%, a combination of both components and a placebo was performed on 51 ragweed sensitive patients presenting allergic conjunctivitis. Evaluation of response at various times after instillation of medication for lacrimation, conjunctival inflammation, pruritus, photophobia and pain showed naphazoline hydrochloride, antazoline phosphate and the combination product superior to placebo. The combination product was statistically significantly superior for conjunctival inflammation and photophobia. The need for post-challenge treatment with epinephrine hydrochloride was significantly less in those eyes treated with the combination product. demonstrating prophylactic efficacy.", "contents": "Antazoline phosphate and naphazoline hydrochloride, singly and in combination for the treatment of allergic conjunctivitis-a controlled, double-blind clinical trial. A controlled, double-blind comparison of naphalzoline hydrochloride 0.05%, antazoline phosphate 0.5%, a combination of both components and a placebo was performed on 51 ragweed sensitive patients presenting allergic conjunctivitis. Evaluation of response at various times after instillation of medication for lacrimation, conjunctival inflammation, pruritus, photophobia and pain showed naphazoline hydrochloride, antazoline phosphate and the combination product superior to placebo. The combination product was statistically significantly superior for conjunctival inflammation and photophobia. The need for post-challenge treatment with epinephrine hydrochloride was significantly less in those eyes treated with the combination product. demonstrating prophylactic efficacy."}
{"id": "PMID:1096686", "title": "Pyridoxine treatment of childhood bronchial asthma.", "content": "Urinary xanthurenic and kynurenic acid levels were measured in five patients while they were receiving 50 mg and 100 mg of pyridoxine. The levels of tryptophane metabolite decreased progressively as the dose was increased but remained above basal levels. There was marked clinical improvement in these patients while receiving the higher dose only. The double-blind study with 76 asthmatic children followed for five months indicated significant improvement in asthma following pyridoxine therapy (200 mg daily) and reduction in dosage of bronchodilators and cortisone. The data suggest that these children with severe bronchial asthma had a metabolic block in tryptophane metabolism, which was benefitted by long-term treatment with large doses of pyridoxine.", "contents": "Pyridoxine treatment of childhood bronchial asthma. Urinary xanthurenic and kynurenic acid levels were measured in five patients while they were receiving 50 mg and 100 mg of pyridoxine. The levels of tryptophane metabolite decreased progressively as the dose was increased but remained above basal levels. There was marked clinical improvement in these patients while receiving the higher dose only. The double-blind study with 76 asthmatic children followed for five months indicated significant improvement in asthma following pyridoxine therapy (200 mg daily) and reduction in dosage of bronchodilators and cortisone. The data suggest that these children with severe bronchial asthma had a metabolic block in tryptophane metabolism, which was benefitted by long-term treatment with large doses of pyridoxine."}
{"id": "PMID:1096687", "title": "Protection against neonatal enteric colibacillosis in pigs suckling orally vaccinated sows.", "content": "Pregnant swine were vaccinated with 1 of 2 enteropathogenic strains of Escherichia coli, and their pigs were challenge inoculated with the homologous strain 1.5 hours after the entire litter was born. Seventeen sows were vaccinated orally by feeding viable cultures on 3 consecutive days, 6 sows were given 2 intramuscular (IM) injections of viable cultures at 10- to 14-day intervals, 6 sows were given 2 IM injections fo formolized cultures at 10- to 14-day intervals, and 12 sows were not vaccinated. The pigs from sows which had been orally vaccinated with viable cultures were protected during the 10-day observation period against diarrhea, as well as against death, when the newborn pigs were challenge inoculated with the homologous strain. Most of the pigs from sows which had been vaccinated IM were protected against death, but few neonates were protected against the diarrheal effects of challenge exposure with the homologous strain. Challenge-inoculated pigs suckling nonvaccinated sows had diarrhea and became dehydrated, and many died. Fewer viable E coli were usually recovered from the homogenized intestinal contents or intestinal segments of newborn pigs which did not have diarrhea than from similar specimens of diarrheal pigs. Microscopic examination of segments of the small intestine revealed that large numbers of E coli were closely associated with the ileal mucosa of newborn pigs killed in the acute phase of neonatal enteric colibacillosis caused by either strain, but organisms were not detected in this location in the pigs which remained normal after challenge exposure. It is concluded that swine which have been vaccinated by feeding large numbers of viable E coli late in gestation can provide good protection to their suckling pigs against the effects of challenge inoculation with the homologous enteropathogenic strain.", "contents": "Protection against neonatal enteric colibacillosis in pigs suckling orally vaccinated sows. Pregnant swine were vaccinated with 1 of 2 enteropathogenic strains of Escherichia coli, and their pigs were challenge inoculated with the homologous strain 1.5 hours after the entire litter was born. Seventeen sows were vaccinated orally by feeding viable cultures on 3 consecutive days, 6 sows were given 2 intramuscular (IM) injections of viable cultures at 10- to 14-day intervals, 6 sows were given 2 IM injections fo formolized cultures at 10- to 14-day intervals, and 12 sows were not vaccinated. The pigs from sows which had been orally vaccinated with viable cultures were protected during the 10-day observation period against diarrhea, as well as against death, when the newborn pigs were challenge inoculated with the homologous strain. Most of the pigs from sows which had been vaccinated IM were protected against death, but few neonates were protected against the diarrheal effects of challenge exposure with the homologous strain. Challenge-inoculated pigs suckling nonvaccinated sows had diarrhea and became dehydrated, and many died. Fewer viable E coli were usually recovered from the homogenized intestinal contents or intestinal segments of newborn pigs which did not have diarrhea than from similar specimens of diarrheal pigs. Microscopic examination of segments of the small intestine revealed that large numbers of E coli were closely associated with the ileal mucosa of newborn pigs killed in the acute phase of neonatal enteric colibacillosis caused by either strain, but organisms were not detected in this location in the pigs which remained normal after challenge exposure. It is concluded that swine which have been vaccinated by feeding large numbers of viable E coli late in gestation can provide good protection to their suckling pigs against the effects of challenge inoculation with the homologous enteropathogenic strain."}
{"id": "PMID:1096681", "title": "Anesthesia and subarachnoid intracranial pressure.", "content": "Intracranial pressure (ICP) was continuously monitored by the Richmond technic of Vries and Becker in 17 patients undergoing elective craniotomy. This method entails the placement, underlocal anesthesia, of a hollow screw through the cranium into the subarachnoid space. The screw was connected to a Statham P23Db pressure transducer with heavy vinyl tubing and with pressures recorded on a Beckman Dynograph. The effects of 3 anesthetic technice-halothane, enflurane, and nitrous oxide-narcotic-relaxant-on ICP during induction and maintenance were compared with preinduction control pressures. Control ICP in awake, lightly premedicated patients was 15 plus or minus 10 torr. Mask inductions with halothane and enflurane consistently caused significant increases in ICP from preinduction levels in the absence of excitement or airway obstruction. Induction with nitrous oxide-narcotic-relaxant did not increase ICP. Decreases in ICP following barbiturate administration were noted. Addition of halothane and enflurane to the inspired mixture of patients controlled and hyperventilated with nitrous oxide and oxygen caused consistent increases in ICP. With control hyperventilation (Paco2 25 plus or minus 5 torr), the ICP did not return toward preinduction values within 5 minutes with enflurane and halothane.", "contents": "Anesthesia and subarachnoid intracranial pressure. Intracranial pressure (ICP) was continuously monitored by the Richmond technic of Vries and Becker in 17 patients undergoing elective craniotomy. This method entails the placement, underlocal anesthesia, of a hollow screw through the cranium into the subarachnoid space. The screw was connected to a Statham P23Db pressure transducer with heavy vinyl tubing and with pressures recorded on a Beckman Dynograph. The effects of 3 anesthetic technice-halothane, enflurane, and nitrous oxide-narcotic-relaxant-on ICP during induction and maintenance were compared with preinduction control pressures. Control ICP in awake, lightly premedicated patients was 15 plus or minus 10 torr. Mask inductions with halothane and enflurane consistently caused significant increases in ICP from preinduction levels in the absence of excitement or airway obstruction. Induction with nitrous oxide-narcotic-relaxant did not increase ICP. Decreases in ICP following barbiturate administration were noted. Addition of halothane and enflurane to the inspired mixture of patients controlled and hyperventilated with nitrous oxide and oxygen caused consistent increases in ICP. With control hyperventilation (Paco2 25 plus or minus 5 torr), the ICP did not return toward preinduction values within 5 minutes with enflurane and halothane."}
{"id": "PMID:1096688", "title": "Enteric colibacillosis: evaluation of strains of Escherichia coli utilizing the ligated loop technique in gnotobiotic swine.", "content": "The enteropathogenicity of 6 strains of Escherichia coli was evaluated via the ligated loop technique in 15 gnotobiotic swine 3 to 4 weeks old. Strain enteropathogenicity in gnotobiotic swine, as determined on the basis of visual loop distention and light microscopic evaluation of intestinal sections, compared favorably with that reported in conventional swine, except that jejunal loops of gnotobiotic swine may be somewhat less sensitive to certain strains. In addition, postoperative mortality rate among gnotobiotic pigs was greater than that reported in conventional pigs. Two types of aberrant loop reactions were observed during the experiment. The 1st, and the more commonly encountered type, was due to the leakage of enteropathogenic organisms or enterotoxin from one loop to another; this was considered to be a true false-positive reaction. The 2nd type of unexpected positive reaction occurred in 2 sacs inoculated with the nonenteropathogenic strain 115. This latter reaction may have represented either a reversion of the organism to its previous enteropathogenic state or a unique reaction of the gnotobiotic intestine to this strain. False-positive loops were not identified in any other sacs, whether inoculated or not. Results of these experiments support the hypothesis that false-positive loops originate from naturally occurring infection.", "contents": "Enteric colibacillosis: evaluation of strains of Escherichia coli utilizing the ligated loop technique in gnotobiotic swine. The enteropathogenicity of 6 strains of Escherichia coli was evaluated via the ligated loop technique in 15 gnotobiotic swine 3 to 4 weeks old. Strain enteropathogenicity in gnotobiotic swine, as determined on the basis of visual loop distention and light microscopic evaluation of intestinal sections, compared favorably with that reported in conventional swine, except that jejunal loops of gnotobiotic swine may be somewhat less sensitive to certain strains. In addition, postoperative mortality rate among gnotobiotic pigs was greater than that reported in conventional pigs. Two types of aberrant loop reactions were observed during the experiment. The 1st, and the more commonly encountered type, was due to the leakage of enteropathogenic organisms or enterotoxin from one loop to another; this was considered to be a true false-positive reaction. The 2nd type of unexpected positive reaction occurred in 2 sacs inoculated with the nonenteropathogenic strain 115. This latter reaction may have represented either a reversion of the organism to its previous enteropathogenic state or a unique reaction of the gnotobiotic intestine to this strain. False-positive loops were not identified in any other sacs, whether inoculated or not. Results of these experiments support the hypothesis that false-positive loops originate from naturally occurring infection."}
{"id": "PMID:1096689", "title": "Enzootic occurrence of chloramphenicol-resistant Salmonella typhimurium var copenhagen in calf population.", "content": "Chloramphenicol (CP)-resistant Salmonella typhimurium var copenhagen was frequently isolated (44.1 to 76.7%) from dead, diseased, and healthy calves on 3 farms in 1973 in northern Japan. On 2 of the farms, CP-resistant salmonella appeared suddenly during an epizootic of salmonella infection, and persisted. Of 87 CP-resistant to tetracycline (TC), streptomycin (SM), and sulfonamides (SA), and the remaining 13 isolates from dead calves on a farm were resistant to TC, SM, SA, and kanamycin (KM). Resistance patterns of all CP-resistant isolates were transferred completely.", "contents": "Enzootic occurrence of chloramphenicol-resistant Salmonella typhimurium var copenhagen in calf population. Chloramphenicol (CP)-resistant Salmonella typhimurium var copenhagen was frequently isolated (44.1 to 76.7%) from dead, diseased, and healthy calves on 3 farms in 1973 in northern Japan. On 2 of the farms, CP-resistant salmonella appeared suddenly during an epizootic of salmonella infection, and persisted. Of 87 CP-resistant to tetracycline (TC), streptomycin (SM), and sulfonamides (SA), and the remaining 13 isolates from dead calves on a farm were resistant to TC, SM, SA, and kanamycin (KM). Resistance patterns of all CP-resistant isolates were transferred completely."}
{"id": "PMID:1096690", "title": "Concentration of lactoferrin in milk of normal lactating cows and changes occurring during mastitis.", "content": "The mean lactoferrin (Lf) concentration determined by electroimmunodiffusion (EID) assay of whey preparations from 80 quarters of 20 normal lactating cows was 0.35 mg/ml. The mean alpha-lactalbumin (alpha-LAC) and bovine serum albumin (BSA) concentrations were 2.01 mg/ml and 0.29 mg/ml, respectively. The mean was significantly related to cell count (P smaller than 0.01), BSA (P smaller than 0.05), stage of lactation (P smaller than 0.05), and milk production (P smaller than 0.05). The Lf-milk production relationship was the only negative correlation. In 11 cows with mastitis, there was a significant (P smaller than 0.01) increase in mean Lf concentration in infected quarters from 0.55 mg/ml on day 1 of the infection to 1.89 mg/ml by day 3. By day 15 clinical signs had subsided and mean Lf concentrations had decreased to near day 1 values. On day 3 quarters infected with coliform bacteria (clinical mastitis generally more severe) had mean Lf values more than twofold greater than those quarters infected with species of Staphylococcus or Streptococcus (milder clinical signs). Noninfected (control) quarters of cows having coliform bacteria-infected quarters had slightly increased mean Lf concentrations, where Lf concentration in contral quarters of cows having quarters infected with gram-positive organisms remained unchanged.", "contents": "Concentration of lactoferrin in milk of normal lactating cows and changes occurring during mastitis. The mean lactoferrin (Lf) concentration determined by electroimmunodiffusion (EID) assay of whey preparations from 80 quarters of 20 normal lactating cows was 0.35 mg/ml. The mean alpha-lactalbumin (alpha-LAC) and bovine serum albumin (BSA) concentrations were 2.01 mg/ml and 0.29 mg/ml, respectively. The mean was significantly related to cell count (P smaller than 0.01), BSA (P smaller than 0.05), stage of lactation (P smaller than 0.05), and milk production (P smaller than 0.05). The Lf-milk production relationship was the only negative correlation. In 11 cows with mastitis, there was a significant (P smaller than 0.01) increase in mean Lf concentration in infected quarters from 0.55 mg/ml on day 1 of the infection to 1.89 mg/ml by day 3. By day 15 clinical signs had subsided and mean Lf concentrations had decreased to near day 1 values. On day 3 quarters infected with coliform bacteria (clinical mastitis generally more severe) had mean Lf values more than twofold greater than those quarters infected with species of Staphylococcus or Streptococcus (milder clinical signs). Noninfected (control) quarters of cows having coliform bacteria-infected quarters had slightly increased mean Lf concentrations, where Lf concentration in contral quarters of cows having quarters infected with gram-positive organisms remained unchanged."}
{"id": "PMID:1096692", "title": "Subacute staphylococcal pneumonia in a renal transplant recipient.", "content": "A patient is reported who developed subacute staphylococcal pneumonia 17 months after renal transplantation. Although the illness was modified by oral antimicrobial drugs, a remarkably benign course was observed, leading to a presumptive diagnosis of opportunistic fungal infection. Thus, staphylococcal pneumonia may complicate the differential diagnosis of pulmonary infection in transplant recipients.", "contents": "Subacute staphylococcal pneumonia in a renal transplant recipient. A patient is reported who developed subacute staphylococcal pneumonia 17 months after renal transplantation. Although the illness was modified by oral antimicrobial drugs, a remarkably benign course was observed, leading to a presumptive diagnosis of opportunistic fungal infection. Thus, staphylococcal pneumonia may complicate the differential diagnosis of pulmonary infection in transplant recipients."}
{"id": "PMID:1096693", "title": "Disseminated mycobacterium kansasii infection presenting as cellulitis in a recipient of a renal homograft.", "content": "A recipient of a renal homograft developed disseminated infection caused by Mycobacterium kansaii. He initially presented with cellulitis and abscesses in one foot, and was thought to have a pyogenic bacterial infection. The daily administration of prednisone and azathioprine appears to have prevented the typical cell-mediated granulomatous reaction to mycobacterial infection and to have contributed to the patient's atypical inflammatory response. A switch to alternate-day prednisone combined with antimycobacterial medication resulted in rapid healing without rejection of the homograft.", "contents": "Disseminated mycobacterium kansasii infection presenting as cellulitis in a recipient of a renal homograft. A recipient of a renal homograft developed disseminated infection caused by Mycobacterium kansaii. He initially presented with cellulitis and abscesses in one foot, and was thought to have a pyogenic bacterial infection. The daily administration of prednisone and azathioprine appears to have prevented the typical cell-mediated granulomatous reaction to mycobacterial infection and to have contributed to the patient's atypical inflammatory response. A switch to alternate-day prednisone combined with antimycobacterial medication resulted in rapid healing without rejection of the homograft."}
{"id": "PMID:1096694", "title": "The pulmonary response to a bacteremic challenge.", "content": "The pulmonary response to a bacteremic challenge was studied in a mouse model. The distribution of intravenously injected radiotracer-labeled Proteus mirabilis and Staphylococcus aureus in the lungs, liver, spleen, kidney, and blood was examined at 0, 5 30, 60, and 240 min. Pulmonary killing of these organisms was studied at 0, 30, and 240 min; 0.8 plus or minus 0.1 per cent of the P. mirabilis and 1.5 plus or minus 0.2 per cent of the S. aureus remained within the lungs after 4 hours. Although only 2.2 plus or minus 0.2 per cent of the P. mirabilis organisms were alive, 33.8 plus or minus 8.8 per cent of the staphylococci remained viable after this period. Light and electron micographs verified that polymorphonuclear leukocytes phagocytized these bacteria. The defense mechanisms of the lung against bloodborne and airborne bacterial infection are functionally, as well as morphologically, distinct.", "contents": "The pulmonary response to a bacteremic challenge. The pulmonary response to a bacteremic challenge was studied in a mouse model. The distribution of intravenously injected radiotracer-labeled Proteus mirabilis and Staphylococcus aureus in the lungs, liver, spleen, kidney, and blood was examined at 0, 5 30, 60, and 240 min. Pulmonary killing of these organisms was studied at 0, 30, and 240 min; 0.8 plus or minus 0.1 per cent of the P. mirabilis and 1.5 plus or minus 0.2 per cent of the S. aureus remained within the lungs after 4 hours. Although only 2.2 plus or minus 0.2 per cent of the P. mirabilis organisms were alive, 33.8 plus or minus 8.8 per cent of the staphylococci remained viable after this period. Light and electron micographs verified that polymorphonuclear leukocytes phagocytized these bacteria. The defense mechanisms of the lung against bloodborne and airborne bacterial infection are functionally, as well as morphologically, distinct."}
{"id": "PMID:1096695", "title": "Learning pulmonary function interpretation: deductive versus inductive methods.", "content": "Volunteer undergraduate medical students were randomly divided into 2 groups. One group were given pulmonary function data and the correct diagnoses from 18 patients. They were told to try to derive the rules by which the diagnoses were made. The other group were given the data from the same 18 patients, but were required to use a computer-assisted instructional program to arrive at the diagnoses in a deductive fashion. Both groups were pre- and post-tested. The deductive method provided higher post-test results. The inductive method took longer and produced more dropouts from the study.", "contents": "Learning pulmonary function interpretation: deductive versus inductive methods. Volunteer undergraduate medical students were randomly divided into 2 groups. One group were given pulmonary function data and the correct diagnoses from 18 patients. They were told to try to derive the rules by which the diagnoses were made. The other group were given the data from the same 18 patients, but were required to use a computer-assisted instructional program to arrive at the diagnoses in a deductive fashion. Both groups were pre- and post-tested. The deductive method provided higher post-test results. The inductive method took longer and produced more dropouts from the study."}
{"id": "PMID:1096699", "title": "Health hazards of bivalve-mollusk ingestion.", "content": "Bivalve mollusks (oysters, clans, and mussels) filter large quantities of water unselectively and thereby may concentrate a variety of aquatic contaminants pathogenic for man within edible shellfish viscera. The recognized bacterial disease associated with ingestion of contaminated bivalves include typhoid fever (not presently a public health problem), Vibrio parahemolyticus gastroenteritis, and Vibrio chloerae infection. The major known shellfish-associated viral diseases are viral hepatitis and possibly viral gastroenteritis. The ingestion of bivalves that have fed on the toxic species of dinoflagellates that produce red tides may be responsible for an uncommon and very rarely fatal illness, paralytic shellfish poisoning. Outbreaks of airborne respiratory irritation in populations exposed to red tides may be the most common public health problem associated with red tides. The health hazards resulting from industrial, agricultural, and oil pollution of bivalves in coastal waters and the hazard from improper handling of bacterially contaminated mollusks remain to be defined.", "contents": "Health hazards of bivalve-mollusk ingestion. Bivalve mollusks (oysters, clans, and mussels) filter large quantities of water unselectively and thereby may concentrate a variety of aquatic contaminants pathogenic for man within edible shellfish viscera. The recognized bacterial disease associated with ingestion of contaminated bivalves include typhoid fever (not presently a public health problem), Vibrio parahemolyticus gastroenteritis, and Vibrio chloerae infection. The major known shellfish-associated viral diseases are viral hepatitis and possibly viral gastroenteritis. The ingestion of bivalves that have fed on the toxic species of dinoflagellates that produce red tides may be responsible for an uncommon and very rarely fatal illness, paralytic shellfish poisoning. Outbreaks of airborne respiratory irritation in populations exposed to red tides may be the most common public health problem associated with red tides. The health hazards resulting from industrial, agricultural, and oil pollution of bivalves in coastal waters and the hazard from improper handling of bacterially contaminated mollusks remain to be defined."}
{"id": "PMID:1096701", "title": "The monoclonal nature of lymphocytes in multiple myeloma. Effects of therapy.", "content": "Despite the increased amounts of serum immumoglobulins (Ig) in multiple myeloma, the percentage of bone marrow-dependent (B) cells carrying surface Ig in the peripheral blood compartment were decreased, when compared with that in control patients with polyclonal gammopathy or with that in normals (5 plus or minus 2% in patients with multiple myeloma and 19 plus or minus 5% or 22 plus or minus 7% in controls). Most circulating B cells in patients with multiple myeloma were shown to carry individually specific idiotypic Ig. The monoclonal idiotypic nature of the surface Ig was shown by using antiserums raised against the monoclonal Ig. The idiotypic antiserum was capable of actively binding to and stimulating the idiotypic lymphocytes to divide in vitro. Commercial anti-Ig antiserums raised against normal Ig did not bind to the myeloma lymphocytes. After chemotherapy, lymphocytes carrying the idiotypic Ig decreased in numbers, and this correlated with other variables of clinical improvements. Its role in the pathogenesis of myeloma and its importance for diagnosis and follow-up of patients with myeloma are discussed.", "contents": "The monoclonal nature of lymphocytes in multiple myeloma. Effects of therapy. Despite the increased amounts of serum immumoglobulins (Ig) in multiple myeloma, the percentage of bone marrow-dependent (B) cells carrying surface Ig in the peripheral blood compartment were decreased, when compared with that in control patients with polyclonal gammopathy or with that in normals (5 plus or minus 2% in patients with multiple myeloma and 19 plus or minus 5% or 22 plus or minus 7% in controls). Most circulating B cells in patients with multiple myeloma were shown to carry individually specific idiotypic Ig. The monoclonal idiotypic nature of the surface Ig was shown by using antiserums raised against the monoclonal Ig. The idiotypic antiserum was capable of actively binding to and stimulating the idiotypic lymphocytes to divide in vitro. Commercial anti-Ig antiserums raised against normal Ig did not bind to the myeloma lymphocytes. After chemotherapy, lymphocytes carrying the idiotypic Ig decreased in numbers, and this correlated with other variables of clinical improvements. Its role in the pathogenesis of myeloma and its importance for diagnosis and follow-up of patients with myeloma are discussed."}
{"id": "PMID:1096702", "title": "The immunofluorescent \"band\" test in mixed connective tissue disease.", "content": "Skin biopsy specimens from normal subjects and patients with mixed connective tissue disease, systemic lupus erythematosus, and rheumatoid arthritis were examined for the presence of a \"band\" of immunoglobulins and complement at the dermal-epidermal junction, using immunofluorescent techniques. A positive immunofluorescent band test was found in the clinically uninvolved skin in three of six patients with mixed connective tissue disease, five of six patients with systemic lupus erythematosus, and none of the patients with rheumatoid arthritis or normal subjects. Although a positive immunofluorescent band test in uninvolved skin has been considered to be fairly specific for the diagnosis of systemic lupus erythematosus, our results suggest that this test cannot be used to distinguish mixed connective tissue disease from systemic lupus erythematosus.", "contents": "The immunofluorescent \"band\" test in mixed connective tissue disease. Skin biopsy specimens from normal subjects and patients with mixed connective tissue disease, systemic lupus erythematosus, and rheumatoid arthritis were examined for the presence of a \"band\" of immunoglobulins and complement at the dermal-epidermal junction, using immunofluorescent techniques. A positive immunofluorescent band test was found in the clinically uninvolved skin in three of six patients with mixed connective tissue disease, five of six patients with systemic lupus erythematosus, and none of the patients with rheumatoid arthritis or normal subjects. Although a positive immunofluorescent band test in uninvolved skin has been considered to be fairly specific for the diagnosis of systemic lupus erythematosus, our results suggest that this test cannot be used to distinguish mixed connective tissue disease from systemic lupus erythematosus."}
{"id": "PMID:1096697", "title": "[Surgical malabsortion (author's transl)].", "content": "Two interesting aspects should be considered in intestinal malabsortion from the Surgical point of view. First, this sindrome may be caused by subobstructive lesions of the gut, which may be able of surgical correction. Second, malabsortion may follow certain operations performed in the gastrointestinal tract. Many of this problems are connected with alterations of the intestinal flora, and or a diminution in the obstructive intestinal surface. The clinical picture is not clear due to the pathological and surgical implications. The experience of the last 10 years is reviewed and conected with the following items, whem accompanied by not infectious diarrhea: a) Hiatus hernia and vaguectomy; b) Gastrointestinal by-pass; c) Intestinal atresia and stenosis; d) Small gut resections-Short bowel sindrome; e) Incompetence of the ileocecal valve.", "contents": "[Surgical malabsortion (author's transl)]. Two interesting aspects should be considered in intestinal malabsortion from the Surgical point of view. First, this sindrome may be caused by subobstructive lesions of the gut, which may be able of surgical correction. Second, malabsortion may follow certain operations performed in the gastrointestinal tract. Many of this problems are connected with alterations of the intestinal flora, and or a diminution in the obstructive intestinal surface. The clinical picture is not clear due to the pathological and surgical implications. The experience of the last 10 years is reviewed and conected with the following items, whem accompanied by not infectious diarrhea: a) Hiatus hernia and vaguectomy; b) Gastrointestinal by-pass; c) Intestinal atresia and stenosis; d) Small gut resections-Short bowel sindrome; e) Incompetence of the ileocecal valve."}
{"id": "PMID:1096703", "title": "Renal vein thrombosis and nephrotic syndrome. A prospective study of 36 adult patients.", "content": "During 2 years we studied 36 of 41 consecutive patients with nephrotic syndrome. Twelve (Group 1) had renal vein thrombosis; the renal pathology was membranous glomerulonephritis in 10 and membranoproliferative glomerulonephritis in 2. Eighteen without renal vein thrombosis (Group 2) had either membranous (10 patients) or membranoproliferative (8 patients) glomerulonephritis. Clinical presentation, laboratory data, renal function, renal histology, and immunopathology were similar in both groups; thromboembolic phenomena were frequent. Ten of 12 patients with renal vein thrombosis were given oral anticoagulants and followed for 4 to 24 months. None died or had thromboembolic episodes. The results show that one third of the patients with nephrotic syndrome associated with either membranous or membranoproliferative glomerulonephritis had renal vein thrombosis, and there were no clinical, laboratory, histological, or immunopathological finding(s) diagnostic of renal vein thrombosis. Our data indicate that renal vein thrombosis is a frequent complication in nephrotic syndrome and membranous or membranoproliferative glomerulonephritis.", "contents": "Renal vein thrombosis and nephrotic syndrome. A prospective study of 36 adult patients. During 2 years we studied 36 of 41 consecutive patients with nephrotic syndrome. Twelve (Group 1) had renal vein thrombosis; the renal pathology was membranous glomerulonephritis in 10 and membranoproliferative glomerulonephritis in 2. Eighteen without renal vein thrombosis (Group 2) had either membranous (10 patients) or membranoproliferative (8 patients) glomerulonephritis. Clinical presentation, laboratory data, renal function, renal histology, and immunopathology were similar in both groups; thromboembolic phenomena were frequent. Ten of 12 patients with renal vein thrombosis were given oral anticoagulants and followed for 4 to 24 months. None died or had thromboembolic episodes. The results show that one third of the patients with nephrotic syndrome associated with either membranous or membranoproliferative glomerulonephritis had renal vein thrombosis, and there were no clinical, laboratory, histological, or immunopathological finding(s) diagnostic of renal vein thrombosis. Our data indicate that renal vein thrombosis is a frequent complication in nephrotic syndrome and membranous or membranoproliferative glomerulonephritis."}
{"id": "PMID:1096704", "title": "Long-term therapy for chronic bacteriuria in men. U.S. Public Health Service cooperative study.", "content": "Response to therapy, renal function, and mortality were analyzed in a prospective study of 249 men with bacteriuria followed for up to 10 years. All patients received initial organism-specific antibiotic therapy followed by 2 years of continuous treatment with sulfamethizole, nitrofurantoin, methenamine mandelate, or placebo. Continuous therapy with active drugs delayed recurrence of bacteriuria and reduced acute clinical exacerbations of infection. Patients with pure Escherichia coli bacteriuria, normal intravenous pyelogram, no previous therapy, and a normal prostate had a good prognosis with short-term antibiotic therapy alone. The presence of prostatic or upper urinary tract calculi, pyelonephritic scars, or mixed or enterococcal infections predicted a poor bacteriologic prognosis. In the absence of severe urologic disease or concomitant noninfectious renal disease no patients with persistent bacteriuria developed renal failure. Continuous antibiotic therapy is of value in selected male patients with bacteriuria in reducing recurrence and acute clinical exacerbations of urinary tract infection.", "contents": "Long-term therapy for chronic bacteriuria in men. U.S. Public Health Service cooperative study. Response to therapy, renal function, and mortality were analyzed in a prospective study of 249 men with bacteriuria followed for up to 10 years. All patients received initial organism-specific antibiotic therapy followed by 2 years of continuous treatment with sulfamethizole, nitrofurantoin, methenamine mandelate, or placebo. Continuous therapy with active drugs delayed recurrence of bacteriuria and reduced acute clinical exacerbations of infection. Patients with pure Escherichia coli bacteriuria, normal intravenous pyelogram, no previous therapy, and a normal prostate had a good prognosis with short-term antibiotic therapy alone. The presence of prostatic or upper urinary tract calculi, pyelonephritic scars, or mixed or enterococcal infections predicted a poor bacteriologic prognosis. In the absence of severe urologic disease or concomitant noninfectious renal disease no patients with persistent bacteriuria developed renal failure. Continuous antibiotic therapy is of value in selected male patients with bacteriuria in reducing recurrence and acute clinical exacerbations of urinary tract infection."}
{"id": "PMID:1096705", "title": "Cardiac response to repeated doses of isoproterenol aerosol.", "content": "We studied 18 patients with moderate to severe asthma for cardiac response to repeated doses of isoproterenol aerosol. In only 1 patient was there a significant increase in heart rate immediately after inhalation of isoproterenol aerosol, and it lasted only for 1 minute. In 2 patients increased heart rate was either due to breathholding or to the propellant in the aerosol. There was no cumulative effect on heart rate. Arrhythmias did not develop at any time despite repeated doses of aerosol every 5 to 20 minutes. Peak expiratory flow rate increased 5 minutes after the first dose, and it increased even further after repeated doses; at no time did it decrease after repeated doses.", "contents": "Cardiac response to repeated doses of isoproterenol aerosol. We studied 18 patients with moderate to severe asthma for cardiac response to repeated doses of isoproterenol aerosol. In only 1 patient was there a significant increase in heart rate immediately after inhalation of isoproterenol aerosol, and it lasted only for 1 minute. In 2 patients increased heart rate was either due to breathholding or to the propellant in the aerosol. There was no cumulative effect on heart rate. Arrhythmias did not develop at any time despite repeated doses of aerosol every 5 to 20 minutes. Peak expiratory flow rate increased 5 minutes after the first dose, and it increased even further after repeated doses; at no time did it decrease after repeated doses."}
{"id": "PMID:1096721", "title": "Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movement.", "content": "In this paper I have presented results of experiments in which spindle microtubules were depolymerized by hydrostatic pressure, in order to examine the Inou\u00e9 dynamic equilibrium concept of spindle assembly and the possible role of microtubule depolymerization-polymerization in the movement of chromosomes. Using a newly developed optical hydrostatic pressure chamber, I investigated with polarization microscopy the quantitative effects of pressure on the polymerization of spindle microtubules and, with phase contrast microscopy, the relationship of pressure-induced spindle microtubule depolymerization to chromosome movement in living cells. From results of earlier experiments, principally those of Inou\u00e9 et al. with low temperature and colchicine as microtubule-depolymerizing agents, and from results of my own research, I have concluded that: (1) spindle fiber microtubules are sensitive to depolymerization by pressure (3000-7000 psi), spindle microtubules do exist in a labile equilibrium with a pool of subunits, and the Inou\u00e9 simple equilibrium model does predict changes in spindle microtubule assembly at metaphase induced by pressure; (2) the stability of microtubules depends on the number of \"attached ends;\" (3) the longest interpolar microtubules and the longest chromosomal fiber microtubules regulate the spindle interpolar length and the chromosome-to-pole positions; (4) chromosome velocity is independent of the number of spindle microtubules, as well as of the drag force of the chromosomes; (5) the chromosomal fiber microtubules transmit the forces between the poles and between the chromosomes and the poles; and (6) polymerization of microtubules does produce pushing forces and, if controlled microtubule depolymerization does not actually produce pulling forces, at least it governs the velocity of chromosome-to-pole movement.", "contents": "Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movement. In this paper I have presented results of experiments in which spindle microtubules were depolymerized by hydrostatic pressure, in order to examine the Inou\u00e9 dynamic equilibrium concept of spindle assembly and the possible role of microtubule depolymerization-polymerization in the movement of chromosomes. Using a newly developed optical hydrostatic pressure chamber, I investigated with polarization microscopy the quantitative effects of pressure on the polymerization of spindle microtubules and, with phase contrast microscopy, the relationship of pressure-induced spindle microtubule depolymerization to chromosome movement in living cells. From results of earlier experiments, principally those of Inou\u00e9 et al. with low temperature and colchicine as microtubule-depolymerizing agents, and from results of my own research, I have concluded that: (1) spindle fiber microtubules are sensitive to depolymerization by pressure (3000-7000 psi), spindle microtubules do exist in a labile equilibrium with a pool of subunits, and the Inou\u00e9 simple equilibrium model does predict changes in spindle microtubule assembly at metaphase induced by pressure; (2) the stability of microtubules depends on the number of \"attached ends;\" (3) the longest interpolar microtubules and the longest chromosomal fiber microtubules regulate the spindle interpolar length and the chromosome-to-pole positions; (4) chromosome velocity is independent of the number of spindle microtubules, as well as of the drag force of the chromosomes; (5) the chromosomal fiber microtubules transmit the forces between the poles and between the chromosomes and the poles; and (6) polymerization of microtubules does produce pushing forces and, if controlled microtubule depolymerization does not actually produce pulling forces, at least it governs the velocity of chromosome-to-pole movement."}
{"id": "PMID:1096733", "title": "Photokeratoscopy in the fitting of soft contact lenses.", "content": "Our ultimate goal is to develop a photokeratoscopic system that will enable the fitter to select an initial lens of the proper design and power to fit a given eye and with reasonable assurance that further fitting will rarely be required. Our studies in this direction have carried us through to a prefitting screening system for the Bausch and Lomb Soflens that greatly increases the probability of successful fitting (and which demonstrates the importance of corneal shape in the successful fit of this lens) to a highly promising experimental system that is applicable to larger lenses with spherical base curves. The development of this computer-assisted fitting system is highly desirable. It will make the fitting a much simpler and efficient process with much less trauma to the eye and a marked reduction in the risk of accidental infection than with trial lens fitting. But, a second desirable effect that is not readily apparent is the use of the computer to drive fabrication equipment to manufacture lenses on a custom-fit basis to obtain the maximum in physiological tolerance that these lenses are capable of delivering. In either case, the patient is the ultimate beneficiary and that should always be our major goal.", "contents": "Photokeratoscopy in the fitting of soft contact lenses. Our ultimate goal is to develop a photokeratoscopic system that will enable the fitter to select an initial lens of the proper design and power to fit a given eye and with reasonable assurance that further fitting will rarely be required. Our studies in this direction have carried us through to a prefitting screening system for the Bausch and Lomb Soflens that greatly increases the probability of successful fitting (and which demonstrates the importance of corneal shape in the successful fit of this lens) to a highly promising experimental system that is applicable to larger lenses with spherical base curves. The development of this computer-assisted fitting system is highly desirable. It will make the fitting a much simpler and efficient process with much less trauma to the eye and a marked reduction in the risk of accidental infection than with trial lens fitting. But, a second desirable effect that is not readily apparent is the use of the computer to drive fabrication equipment to manufacture lenses on a custom-fit basis to obtain the maximum in physiological tolerance that these lenses are capable of delivering. In either case, the patient is the ultimate beneficiary and that should always be our major goal."}
{"id": "PMID:1096734", "title": "A revised Fasanella-Servat ptosis operation.", "content": "The Fasanella-Servat operation for ptosis has had several modifications of suture placement. The present method utilized 5-0 nylon which traverses the cut edges as a running mattress suture. The ends of the suture are fixed on the skin surface with rubber bolsters. The suture is removed in 10 days. The advantages are: (1) no suture knots on the corneal surface; (2) ease of placement; (3) minimal tissue reaction; (4) the suture is removed; and (5) ease of suture removal.", "contents": "A revised Fasanella-Servat ptosis operation. The Fasanella-Servat operation for ptosis has had several modifications of suture placement. The present method utilized 5-0 nylon which traverses the cut edges as a running mattress suture. The ends of the suture are fixed on the skin surface with rubber bolsters. The suture is removed in 10 days. The advantages are: (1) no suture knots on the corneal surface; (2) ease of placement; (3) minimal tissue reaction; (4) the suture is removed; and (5) ease of suture removal."}
{"id": "PMID:1096736", "title": "Levator tuck: a simplified blepharoptosis procedure.", "content": "A simplified technique for tucking the levator palpebrae superioris aponeurosis and muscle in the correction of blepharoptosis is described. The anterior (skin) approach is used and no special instruments are required. The advantages of this procedure are discussed, including preservation of the normal anatomy as much as possible, minimum dissection and minimum edema. A preliminary number of cases are reported. It is believed that this technique is useful in cases of acquired blepharoptosis and in congenital blepharoptosis with good levator function.", "contents": "Levator tuck: a simplified blepharoptosis procedure. A simplified technique for tucking the levator palpebrae superioris aponeurosis and muscle in the correction of blepharoptosis is described. The anterior (skin) approach is used and no special instruments are required. The advantages of this procedure are discussed, including preservation of the normal anatomy as much as possible, minimum dissection and minimum edema. A preliminary number of cases are reported. It is believed that this technique is useful in cases of acquired blepharoptosis and in congenital blepharoptosis with good levator function."}
{"id": "PMID:1096740", "title": "Choledochal cysts. A review of the cystic anomalies of the biliary tree.", "content": "Congenital cysts of the biliary tree, although uncommon, are being reported in increasing numbers. The widely accepted classification would seem to warrant modification in the light of recent observations. The clinical spectrum of the condition is reviewed, together with the surgical approach to treatment and its indications and complications.", "contents": "Choledochal cysts. A review of the cystic anomalies of the biliary tree. Congenital cysts of the biliary tree, although uncommon, are being reported in increasing numbers. The widely accepted classification would seem to warrant modification in the light of recent observations. The clinical spectrum of the condition is reviewed, together with the surgical approach to treatment and its indications and complications."}
{"id": "PMID:1096744", "title": "Anesthesia in the Yom Kippur war.", "content": "The role of the anesthetist in the treatment of battle casualties is discussed in the light of personal experience in a field hospital and in the rear during the Yom Kippur War of October 1973. Resuscitation and intensive care both before and after evacuation play an important part in reducing mortality, and the importance of providing adequate facilities for these functions in the battle area as well as at the base is emphasized.", "contents": "Anesthesia in the Yom Kippur war. The role of the anesthetist in the treatment of battle casualties is discussed in the light of personal experience in a field hospital and in the rear during the Yom Kippur War of October 1973. Resuscitation and intensive care both before and after evacuation play an important part in reducing mortality, and the importance of providing adequate facilities for these functions in the battle area as well as at the base is emphasized."}
{"id": "PMID:1096757", "title": "Scanning techniques to assess thyroid nodules.", "content": "Despite its nonspecificity, the thyroid scan remains an important diagnostic aid in the evaluation of the patient with a solitary thyroid nodule. Dual radionuclide thyroid imaging using 99m-Tc pertechnetate and 123-I offers useful information than either alone. The role of complementary diagnostic modalities using ultransonography and thermography looks promising but is as yet unclear.", "contents": "Scanning techniques to assess thyroid nodules. Despite its nonspecificity, the thyroid scan remains an important diagnostic aid in the evaluation of the patient with a solitary thyroid nodule. Dual radionuclide thyroid imaging using 99m-Tc pertechnetate and 123-I offers useful information than either alone. The role of complementary diagnostic modalities using ultransonography and thermography looks promising but is as yet unclear."}
{"id": "PMID:1096760", "title": "Selective distal splenorenal shunt for bleeding esophageal varices.", "content": "The selective distal splenorenal shunt has been shown to be equally as effective as total portal-systemic shunting procedures in the control of variceal hemorrhage and it can now be performed with an equivalent operative mortality. It is superior to other shunts in metabolic aspects and results in a markedly decreased incidence of postoperative hepatic encephalopathy. Massive ascites constitutes a definite contraindication to the selective distal splenorenal anastomosis, but for all other suitable patients this procedure appears to be the optimal method of surgical management at this time.", "contents": "Selective distal splenorenal shunt for bleeding esophageal varices. The selective distal splenorenal shunt has been shown to be equally as effective as total portal-systemic shunting procedures in the control of variceal hemorrhage and it can now be performed with an equivalent operative mortality. It is superior to other shunts in metabolic aspects and results in a markedly decreased incidence of postoperative hepatic encephalopathy. Massive ascites constitutes a definite contraindication to the selective distal splenorenal anastomosis, but for all other suitable patients this procedure appears to be the optimal method of surgical management at this time."}
{"id": "PMID:1096763", "title": "Low renin hypertension.", "content": "Low renin hypertension comprises a spectrum of disorders ranging from primary aldosteronism to obvious disorders of other mineralocorticoids, as well as a variety of miscellaneous disorders. The largest group of patients with low renin hypertension have no clear abnormality in mineralocorticoid production. However, many lines of evidence suggest the critical role of volume excess in the pathogenesis of hypertension in these patients. More detailed physiological studies must be performed in order to totally understand the spectrum of pathophysiology in low renin hypertension. However, while such studies are in progress the evidence from the literature suggests that these patients must be treated and that in most of these patients diuretic administration, either spironolactone or the thiazide group of diuretics, is usually effective in achieving a reduction of blood pressure to normal.", "contents": "Low renin hypertension. Low renin hypertension comprises a spectrum of disorders ranging from primary aldosteronism to obvious disorders of other mineralocorticoids, as well as a variety of miscellaneous disorders. The largest group of patients with low renin hypertension have no clear abnormality in mineralocorticoid production. However, many lines of evidence suggest the critical role of volume excess in the pathogenesis of hypertension in these patients. More detailed physiological studies must be performed in order to totally understand the spectrum of pathophysiology in low renin hypertension. However, while such studies are in progress the evidence from the literature suggests that these patients must be treated and that in most of these patients diuretic administration, either spironolactone or the thiazide group of diuretics, is usually effective in achieving a reduction of blood pressure to normal."}
{"id": "PMID:1096768", "title": "The steal syndromes.", "content": "Blood can be stolen from almost any vascular bed and redistributed. The external carotid artery can steal from cerebral structures when occlusion of the common carotid artery proximal to the bifurcation causes reversal of flow through the internal carotid artery (13, 45, 46). The external carotid artery has also been shown to steal blood flow from the vertebral artery distribution (13, 74). Double steals involving both the subclavian and external carotid arteries are seen in 1 to 2% of patients (13, 46). There is also the \"triple steal syndrome\" wherein both subclavian arteries and one external carotid artery are involved (14).", "contents": "The steal syndromes. Blood can be stolen from almost any vascular bed and redistributed. The external carotid artery can steal from cerebral structures when occlusion of the common carotid artery proximal to the bifurcation causes reversal of flow through the internal carotid artery (13, 45, 46). The external carotid artery has also been shown to steal blood flow from the vertebral artery distribution (13, 74). Double steals involving both the subclavian and external carotid arteries are seen in 1 to 2% of patients (13, 46). There is also the \"triple steal syndrome\" wherein both subclavian arteries and one external carotid artery are involved (14)."}
{"id": "PMID:1096769", "title": "Von Willebrand's disease.", "content": "The common inheritable hemorrhagic disorder, von Willebrand's disease, has been known for only about 50 yr. During these years it has been well established that the cause of the disease is a deficiency of a plasma protein with remarkable biological properties. Future research should focus on details of the molecular structure of this protein. It is hoped that basic science will help to elucidate those aspects of von Willebrand's disease that are not yet properly understood.", "contents": "Von Willebrand's disease. The common inheritable hemorrhagic disorder, von Willebrand's disease, has been known for only about 50 yr. During these years it has been well established that the cause of the disease is a deficiency of a plasma protein with remarkable biological properties. Future research should focus on details of the molecular structure of this protein. It is hoped that basic science will help to elucidate those aspects of von Willebrand's disease that are not yet properly understood."}
{"id": "PMID:1096770", "title": "The autinglobulin test in autoimmune hemolytic anemia.", "content": "The foregoing summarizes what can be learned from a carefully performed antiglobulin test using specific antisera. Clinical syndromes can be more easily considered, and mechanisms of destruction can be more certainly discerned.", "contents": "The autinglobulin test in autoimmune hemolytic anemia. The foregoing summarizes what can be learned from a carefully performed antiglobulin test using specific antisera. Clinical syndromes can be more easily considered, and mechanisms of destruction can be more certainly discerned."}
{"id": "PMID:1096783", "title": "Metabolic response to infection.", "content": "A generalized infectious process leads to a broad and complex array of metabolic responses within the host. Certain of these responses have a direct causal relationship to the interactions of body cells with invading  microorganisms or their products, or to specific host defensive mechanisms. Other host metabolic responses have less clearly defined roles although they involve many tissues and seem to be initiated and regulated by certain hormones-like endogenous mediators. These latter responses contribute to the maintenance of body homeostasis, the provision of metabolizable energy to meet increased body needs, and the synthesis of the acute phase reactant serum proteins.", "contents": "Metabolic response to infection. A generalized infectious process leads to a broad and complex array of metabolic responses within the host. Certain of these responses have a direct causal relationship to the interactions of body cells with invading  microorganisms or their products, or to specific host defensive mechanisms. Other host metabolic responses have less clearly defined roles although they involve many tissues and seem to be initiated and regulated by certain hormones-like endogenous mediators. These latter responses contribute to the maintenance of body homeostasis, the provision of metabolizable energy to meet increased body needs, and the synthesis of the acute phase reactant serum proteins."}
{"id": "PMID:1096789", "title": "Effects of drugs on uric acid in man.", "content": "In this review the drugs capable of increasing or lowering the serum urate concentration are considered with regard to mechanism of action and significance of the effect, where known.  Several hypouricemic agents, probenecid, sulfinpyrazone, and allopurinol, are considered in further detail because of their major clinical importance.  No effort has been made, however, to summarize the relative clinical indications for each agent.  In addition, in those cases where specific details could not be included because of limitation of space, the appropiate reference has been given.", "contents": "Effects of drugs on uric acid in man. In this review the drugs capable of increasing or lowering the serum urate concentration are considered with regard to mechanism of action and significance of the effect, where known.  Several hypouricemic agents, probenecid, sulfinpyrazone, and allopurinol, are considered in further detail because of their major clinical importance.  No effort has been made, however, to summarize the relative clinical indications for each agent.  In addition, in those cases where specific details could not be included because of limitation of space, the appropiate reference has been given."}
{"id": "PMID:1096791", "title": "Oral contraceptives: relations to mammary cancer, benign breast lesions, and cervical cancer.", "content": "The results of experimental studies on the relationship of estrogens and oral contraceptives to mammary lesions demonstrate that the type of response obtained depends largely on the species and strain of animal that is employed. A variety of clinical studies has failed to demonstrate that estrogen can cause mammary cancer, this lack of effort correlates with the results obtained in various studies in animals. Similar relationships exist for oral contraceptives, and the clinical data show good agreement in demonstrating that the contraceptive steroids do not have a tumorigenic effect on the human mammary gland.  Estrogen can increase the occurrence of cervical cancer in certain strains of mice, but apparently this effect is not observed in other species of animals, including man.  The preponderance of data shows that oral contraceptives do not adversely affect the occurence of abnormal Papanicolaou smears, cervical dysplasia, cervical cancer in situ, or invasive cervical cancer.", "contents": "Oral contraceptives: relations to mammary cancer, benign breast lesions, and cervical cancer. The results of experimental studies on the relationship of estrogens and oral contraceptives to mammary lesions demonstrate that the type of response obtained depends largely on the species and strain of animal that is employed. A variety of clinical studies has failed to demonstrate that estrogen can cause mammary cancer, this lack of effort correlates with the results obtained in various studies in animals. Similar relationships exist for oral contraceptives, and the clinical data show good agreement in demonstrating that the contraceptive steroids do not have a tumorigenic effect on the human mammary gland.  Estrogen can increase the occurrence of cervical cancer in certain strains of mice, but apparently this effect is not observed in other species of animals, including man.  The preponderance of data shows that oral contraceptives do not adversely affect the occurence of abnormal Papanicolaou smears, cervical dysplasia, cervical cancer in situ, or invasive cervical cancer."}
{"id": "PMID:1096799", "title": "Antibiotic resistance patterns of clinical isolates of Serratia marcescens.", "content": "Antimicrobial susceptibility patterns of 102 clinical isolates of Serratia marcescens from three medical centers were studied by using disk sensitivity and agar dilution methods. The least resistance was demonstrated against gentamicin, nalidixic acid, chloramphenicol, and sulfisoxazole, all of which inhibited more than 80% of the strains. Cephalothin was completely ineffective, and more than 90% of strains were resistant to ampicillin and tetracycline. As demonstrated by the agar dilution method, the minimal inhibitory concentration of nalidixic acid, gentamicin, tobramycin, and chloramphenicol for most strains fell within therapeutically attainable concentrations. The prevalence of resistance to ampicillin, cephalothin, and tetracycline was nearly the same at all three medical centers, whereas there appeared to be patterns characteristic for each center with regard to the other drugs used. Eleven of the isolates produced pigment and exhibited patterns similar but not identical to those of the nonpigmented strains, all 11 being resistant to between three and six drugs. Half of the strains were resistant to five or more antibiotics, indicating that some Serratia exhibit resistance to an unusually broad range of therapeutic agents.", "contents": "Antibiotic resistance patterns of clinical isolates of Serratia marcescens. Antimicrobial susceptibility patterns of 102 clinical isolates of Serratia marcescens from three medical centers were studied by using disk sensitivity and agar dilution methods. The least resistance was demonstrated against gentamicin, nalidixic acid, chloramphenicol, and sulfisoxazole, all of which inhibited more than 80% of the strains. Cephalothin was completely ineffective, and more than 90% of strains were resistant to ampicillin and tetracycline. As demonstrated by the agar dilution method, the minimal inhibitory concentration of nalidixic acid, gentamicin, tobramycin, and chloramphenicol for most strains fell within therapeutically attainable concentrations. The prevalence of resistance to ampicillin, cephalothin, and tetracycline was nearly the same at all three medical centers, whereas there appeared to be patterns characteristic for each center with regard to the other drugs used. Eleven of the isolates produced pigment and exhibited patterns similar but not identical to those of the nonpigmented strains, all 11 being resistant to between three and six drugs. Half of the strains were resistant to five or more antibiotics, indicating that some Serratia exhibit resistance to an unusually broad range of therapeutic agents."}
{"id": "PMID:1096800", "title": "Comparative efficacy of cephalexin and ampicillin for shigellosis and other types of acute diarrhea in infants and children.", "content": "Most ampicillin-resistant Shigella are susceptible to cephalexin. Randomized treatment with cephalexin or ampicillin was given to 154 infants and children with acute diarrhea. Rectal swab cultures revealed Shigella in 42%, Salmonella in 6%, enteropathogenic Escherichia coli in 2%, and no pathogen in 50%. Cephalexin failed to eradicate Shigella after 5 days of treatment in 76% of patients as contrasted with 28% of ampicillin-treated patients with susceptible organisms. Shigella persisted in 78% of ampicillin-treated patients with resistant organisms. Diarrhea lasted more than 5 days in 43% of cephalexin-treated patients, in 56% of the ampicillin group with resistant organisms, but in only 9% of ampicillin-treated patients with susceptible organisms. The failure of cephalexin was due to the relatively high minimal inhibitory concentrations and minimal bacterial concentrations of 5 or 10 mug/ml and, although serum concentrations were twice the minimal bacterial concentration, they were not sufficient to demonstrate killing by the serum dilution method. In vitro susceptibility or resistance of Shigella to ampicillin correlated with clinical success or failure. Cephalexin is not a suitable drug for treatment of shigellosis in patients with ampicillin-resistant organisms.", "contents": "Comparative efficacy of cephalexin and ampicillin for shigellosis and other types of acute diarrhea in infants and children. Most ampicillin-resistant Shigella are susceptible to cephalexin. Randomized treatment with cephalexin or ampicillin was given to 154 infants and children with acute diarrhea. Rectal swab cultures revealed Shigella in 42%, Salmonella in 6%, enteropathogenic Escherichia coli in 2%, and no pathogen in 50%. Cephalexin failed to eradicate Shigella after 5 days of treatment in 76% of patients as contrasted with 28% of ampicillin-treated patients with susceptible organisms. Shigella persisted in 78% of ampicillin-treated patients with resistant organisms. Diarrhea lasted more than 5 days in 43% of cephalexin-treated patients, in 56% of the ampicillin group with resistant organisms, but in only 9% of ampicillin-treated patients with susceptible organisms. The failure of cephalexin was due to the relatively high minimal inhibitory concentrations and minimal bacterial concentrations of 5 or 10 mug/ml and, although serum concentrations were twice the minimal bacterial concentration, they were not sufficient to demonstrate killing by the serum dilution method. In vitro susceptibility or resistance of Shigella to ampicillin correlated with clinical success or failure. Cephalexin is not a suitable drug for treatment of shigellosis in patients with ampicillin-resistant organisms."}
{"id": "PMID:1096801", "title": "Rapid, simple bioassay for 5-fluorocytosine in the presence of amphotericin B.", "content": "It is important that serum levels of 5-fluorocytosine (5FC) be measured to insure therapeutic levels while avoiding toxicity. This is particularly true in patients with renal insufficiency. The concurrent use of amphotericin B and 5FC complicates the measurement of 5FC in the serum, since fungi used in conventional bioassay systems are uniformly susceptible to amphotericin B. This paper describes the development of a simple, reliable, 6-h bioassay for 5FC in the presence of amphotericin B. The assay is based upon the fact that 5FC diffuses readily through yeast nitrogen base agar, whereas amphotericin B apparently does not. This assay allows rapid adjustments in therapy of patients receiving both 5FC and amphotericin B and has permitted us to maintain 5FC serum levels between limits of 25 and 120 mug/ml in patients with altered renal function.", "contents": "Rapid, simple bioassay for 5-fluorocytosine in the presence of amphotericin B. It is important that serum levels of 5-fluorocytosine (5FC) be measured to insure therapeutic levels while avoiding toxicity. This is particularly true in patients with renal insufficiency. The concurrent use of amphotericin B and 5FC complicates the measurement of 5FC in the serum, since fungi used in conventional bioassay systems are uniformly susceptible to amphotericin B. This paper describes the development of a simple, reliable, 6-h bioassay for 5FC in the presence of amphotericin B. The assay is based upon the fact that 5FC diffuses readily through yeast nitrogen base agar, whereas amphotericin B apparently does not. This assay allows rapid adjustments in therapy of patients receiving both 5FC and amphotericin B and has permitted us to maintain 5FC serum levels between limits of 25 and 120 mug/ml in patients with altered renal function."}
{"id": "PMID:1096802", "title": "Inhibition by levorphanol and related drugs of amino acid transport by isolated membrane vesicles from Escherichia coli.", "content": "Levorphanol inhibits the transport of the amino acids proline and lysine by cytoplasmic membrane vesicles derived from Escherichia coli. The degree of inhibition increases with increasing levorphanol concentration and ranges from 26% at 10(-6) M levorphanol to 92% at 10(-3) M levorphanol. The effect is independent of the energy source, since levorphanol inhibits proline uptake to the same extent in the presence of 20 mM d-lactate or 20 mM succinate and in the absence of an exogenous energy source. Levorphanol does not irreversibly alter the ability of membrane vesicles to transport proline, since incubation of membrane vesicles for 15 min in the presence of 0.25 mM levorphanol, a concentration which inhibits proline transport by more than 75%, has no effect on the rate of proline transport by these vesicles once the drug is removed. Both the maximum velocity and the K(m) of proline transport are modified by levorphanol, hence, the type of inhibition produced by levorphanol is mixed. The inhibitor constant (K(i)) for levorphanol inhibition of proline transport is approximately 3 x 10(-4) M. Membrane vesicles incubated in the presence of levorphanol accumulate much less proline at the steady state than do control vesicles. Furthermore, the addition of levorphanol to membrane vesicles preloaded to the steady state with proline produces a marked net efflux of proline. Levorphanol does not block either temperature-induced efflux or exchange of external proline with [(14)C]proline present in the intravesicular pool. Dextrorphan, the enantiomorph of levorphanol, and levallorphan, the N-allyl analogue of levorphanol, inhibit proline and lysine transport in a similar manner. Possible mechanisms of the effects of these drugs on cell membranes are discussed.", "contents": "Inhibition by levorphanol and related drugs of amino acid transport by isolated membrane vesicles from Escherichia coli. Levorphanol inhibits the transport of the amino acids proline and lysine by cytoplasmic membrane vesicles derived from Escherichia coli. The degree of inhibition increases with increasing levorphanol concentration and ranges from 26% at 10(-6) M levorphanol to 92% at 10(-3) M levorphanol. The effect is independent of the energy source, since levorphanol inhibits proline uptake to the same extent in the presence of 20 mM d-lactate or 20 mM succinate and in the absence of an exogenous energy source. Levorphanol does not irreversibly alter the ability of membrane vesicles to transport proline, since incubation of membrane vesicles for 15 min in the presence of 0.25 mM levorphanol, a concentration which inhibits proline transport by more than 75%, has no effect on the rate of proline transport by these vesicles once the drug is removed. Both the maximum velocity and the K(m) of proline transport are modified by levorphanol, hence, the type of inhibition produced by levorphanol is mixed. The inhibitor constant (K(i)) for levorphanol inhibition of proline transport is approximately 3 x 10(-4) M. Membrane vesicles incubated in the presence of levorphanol accumulate much less proline at the steady state than do control vesicles. Furthermore, the addition of levorphanol to membrane vesicles preloaded to the steady state with proline produces a marked net efflux of proline. Levorphanol does not block either temperature-induced efflux or exchange of external proline with [(14)C]proline present in the intravesicular pool. Dextrorphan, the enantiomorph of levorphanol, and levallorphan, the N-allyl analogue of levorphanol, inhibit proline and lysine transport in a similar manner. Possible mechanisms of the effects of these drugs on cell membranes are discussed."}
{"id": "PMID:1096803", "title": "Inactivation of Treponema pallidum by silver sulfadiazine.", "content": "Silver sulfadiazine, an anti-infectious agent for the prevention and treatment of burn sepsis, has been found to possess antitreponemal activity against Treponema pallidum. At 28 C, complete inactivation of the organism was produced by exposure of the organism to a concentration of 50 mug of the drug per ml for 1 to 5 min, 12 to 25 mug/ml for 10 to 15 min, and 6.2 mug/ml for 30 min. At 37 C, the amounts of silver sulfadiazine required for inactivation were two- to fourfold less.", "contents": "Inactivation of Treponema pallidum by silver sulfadiazine. Silver sulfadiazine, an anti-infectious agent for the prevention and treatment of burn sepsis, has been found to possess antitreponemal activity against Treponema pallidum. At 28 C, complete inactivation of the organism was produced by exposure of the organism to a concentration of 50 mug of the drug per ml for 1 to 5 min, 12 to 25 mug/ml for 10 to 15 min, and 6.2 mug/ml for 30 min. At 37 C, the amounts of silver sulfadiazine required for inactivation were two- to fourfold less."}
{"id": "PMID:1096804", "title": "Effect of protein concentration and binding on antibiotic assays.", "content": "ASSAY CURVES, USING A DISK DIFFUSION METHOD FOR THE ANTIBIOTICS GENTAMICIN AND CEFAZOLIN, WERE PREPARED WITH: saline, saline plus 10% serum, and ascitic, synovial, cerebrospinal, and pleural fluids. The curves were compared with a standard curve prepared with pooled human serum. The pH, total protein, glucose, blood urea nitrogen, sodium, potassium, calcium, phosphorus, chloride, CO(2) content, uric acid, cholesterol, bilirubin, serum glutamic oxalacetic transaminase, CPK, LDH, and alkaline phosphatase were determined and compared for all fluids. Measurements for cefazolin levels were falsely elevated in those fluids with low protein content when serum was used as a reference standard. There was a linear inverse relationship between the protein content of the fluids and the cefazolin level with serum as the standard for the assay of this highly protein-bound antibiotic. No discrepancies were observed in the assay curves for gentamicin, an antibiotic known not to be bound by serum proteins.", "contents": "Effect of protein concentration and binding on antibiotic assays. ASSAY CURVES, USING A DISK DIFFUSION METHOD FOR THE ANTIBIOTICS GENTAMICIN AND CEFAZOLIN, WERE PREPARED WITH: saline, saline plus 10% serum, and ascitic, synovial, cerebrospinal, and pleural fluids. The curves were compared with a standard curve prepared with pooled human serum. The pH, total protein, glucose, blood urea nitrogen, sodium, potassium, calcium, phosphorus, chloride, CO(2) content, uric acid, cholesterol, bilirubin, serum glutamic oxalacetic transaminase, CPK, LDH, and alkaline phosphatase were determined and compared for all fluids. Measurements for cefazolin levels were falsely elevated in those fluids with low protein content when serum was used as a reference standard. There was a linear inverse relationship between the protein content of the fluids and the cefazolin level with serum as the standard for the assay of this highly protein-bound antibiotic. No discrepancies were observed in the assay curves for gentamicin, an antibiotic known not to be bound by serum proteins."}
{"id": "PMID:1096805", "title": "Recovery of polysome function of T4-infected Escherichia coli after brief treatment with chloramphenicol and rifampin.", "content": "T4-infected Escherichia coli cells briefly exposed to rifampin, or to rifampin plus chloramphenicol, were capable of protein synthesis for some time after removal of the antibiotics, although ribonucleic acid synthesis was irreversibly inhibited. Partially completed peptides trapped on polysomes by high levels of chloramphenicol were eventually completed after removal of the drug, as demonstrated by subjecting labeled peptides from appropriate polysome regions to polyacrylamide disc gel electrophoresis. Thus, the effect of the drug appears to be reversible on the molecular as well as the cellular level.", "contents": "Recovery of polysome function of T4-infected Escherichia coli after brief treatment with chloramphenicol and rifampin. T4-infected Escherichia coli cells briefly exposed to rifampin, or to rifampin plus chloramphenicol, were capable of protein synthesis for some time after removal of the antibiotics, although ribonucleic acid synthesis was irreversibly inhibited. Partially completed peptides trapped on polysomes by high levels of chloramphenicol were eventually completed after removal of the drug, as demonstrated by subjecting labeled peptides from appropriate polysome regions to polyacrylamide disc gel electrophoresis. Thus, the effect of the drug appears to be reversible on the molecular as well as the cellular level."}
{"id": "PMID:1096806", "title": "Stimulation of enzyme synthesis by sublethal concentrations of chloramphenicol is not mediated by ribonucleotide pools.", "content": "Growth of Salmonella typhimurium LT-2 strain pyrA81 in minimal medium containing 0.8 mug of chloramphenicol (CAP) per ml resulted in a 50 to 100% increase in the steady-state nucleotide pools in the cells. When such a culture was starved for uracil, the pyrimidine nucleotide pools decayed much more slowly in CAP-treated cells than in controls. An attempt was made to determine whether this effect of CAP on nucleotide pools could account for enhanced enzyme derepression observed under the same conditions (8). Treatment with low levels of puromycin also resulted in elevation of nucleotide pools but did not lead to enhanced enzyme synthesis. CAP treatment during arginine starvation has been shown to enhance enzyme synthesis, but nucleotide pools were not significantly affected by CAP nor was the stringent response relieved under these conditions. Thus, the effects of CAP on enzyme synthesis cannot be the result of effects of the antibiotic on nucleotide pools in all cases. The elevation of nucleotide pools was shown not to be due to enhanced conversion of exogeneous uracil into nucleotides, but to result from the enhanced turnover of ribonucleic acid in CAP-treated cells.", "contents": "Stimulation of enzyme synthesis by sublethal concentrations of chloramphenicol is not mediated by ribonucleotide pools. Growth of Salmonella typhimurium LT-2 strain pyrA81 in minimal medium containing 0.8 mug of chloramphenicol (CAP) per ml resulted in a 50 to 100% increase in the steady-state nucleotide pools in the cells. When such a culture was starved for uracil, the pyrimidine nucleotide pools decayed much more slowly in CAP-treated cells than in controls. An attempt was made to determine whether this effect of CAP on nucleotide pools could account for enhanced enzyme derepression observed under the same conditions (8). Treatment with low levels of puromycin also resulted in elevation of nucleotide pools but did not lead to enhanced enzyme synthesis. CAP treatment during arginine starvation has been shown to enhance enzyme synthesis, but nucleotide pools were not significantly affected by CAP nor was the stringent response relieved under these conditions. Thus, the effects of CAP on enzyme synthesis cannot be the result of effects of the antibiotic on nucleotide pools in all cases. The elevation of nucleotide pools was shown not to be due to enhanced conversion of exogeneous uracil into nucleotides, but to result from the enhanced turnover of ribonucleic acid in CAP-treated cells."}
{"id": "PMID:1096807", "title": "Effect of tetracycline on puromycin-induced polysome degradation: influence of magnesium and polyamines.", "content": "Puromycin-induced polysome degradation has been shown to require G factor, guanosine 5'-triphosphate, and the presence of a ribosome release factor (Hirashima and Kaji, 1972, 1973). Tetracycline, which does not inhibit formation of peptidyl-puromycin (Gottesman, 1967; Sarkar and Thach, 1968) nor the guanosine 5'-triphosphate hydrolysis mediated by elongation factor Tu (Ono et al., 1969), inhibits polysome degradation. The tetracycline inhibition requires Mg(2+) at concentrations above 8 mM, which are inhibitory to protein synthesis in vitro. At concentrations of Mg(2+) below 8 mM, polysome degradation is insensitive to tetracycline, but not to fusidic acid. Addition of spermidine, but not of other polyamines, enables the tetracycline inhibition to occur at concentrations of Mg(2+) as low as 2 mM. The inhibition by tetracycline and by fusidic acid suggests that ribosome movement may be essential for the function of ribosome release factor, or that these antibiotics may directly affect its action.", "contents": "Effect of tetracycline on puromycin-induced polysome degradation: influence of magnesium and polyamines. Puromycin-induced polysome degradation has been shown to require G factor, guanosine 5'-triphosphate, and the presence of a ribosome release factor (Hirashima and Kaji, 1972, 1973). Tetracycline, which does not inhibit formation of peptidyl-puromycin (Gottesman, 1967; Sarkar and Thach, 1968) nor the guanosine 5'-triphosphate hydrolysis mediated by elongation factor Tu (Ono et al., 1969), inhibits polysome degradation. The tetracycline inhibition requires Mg(2+) at concentrations above 8 mM, which are inhibitory to protein synthesis in vitro. At concentrations of Mg(2+) below 8 mM, polysome degradation is insensitive to tetracycline, but not to fusidic acid. Addition of spermidine, but not of other polyamines, enables the tetracycline inhibition to occur at concentrations of Mg(2+) as low as 2 mM. The inhibition by tetracycline and by fusidic acid suggests that ribosome movement may be essential for the function of ribosome release factor, or that these antibiotics may directly affect its action."}
{"id": "PMID:1096808", "title": "Incorporation of L-2,5-dihydrophenylalanine into cell proteins of Escherichia coli and sarcoma 180.", "content": "l-2,5-Dihydrophenylalanine is extensively incorporated as a phenylalanine analogue into cell proteins. Phenylalanine-requiring Escherichia coli ATCC 9723f and sarcoma 180 grow at normal rates initially with the analogue and maximally replace 65 and 33%, respectively, of phenylalanine in the peptide residues of their cell protein without death. With the analogue alone growth of E. coli becomes non-steady-state and asymptotically inhibited. In mixtures of the analogue and phenylalanine, growth eventually becomes steady state or logarithmic. The logarithmic rate is inversely proportional to the extent of incorporation of this analogue or of p-fluorophenylalanine, and the projected maximal replacement is the same as that obtained asymptotically with the analogue alone. Thus, the toxicities in steady-state and non-steady-state growth are closely related. Moreover, it is proposed that single salient protein defects may determine the extent of growth rate reduction.", "contents": "Incorporation of L-2,5-dihydrophenylalanine into cell proteins of Escherichia coli and sarcoma 180. l-2,5-Dihydrophenylalanine is extensively incorporated as a phenylalanine analogue into cell proteins. Phenylalanine-requiring Escherichia coli ATCC 9723f and sarcoma 180 grow at normal rates initially with the analogue and maximally replace 65 and 33%, respectively, of phenylalanine in the peptide residues of their cell protein without death. With the analogue alone growth of E. coli becomes non-steady-state and asymptotically inhibited. In mixtures of the analogue and phenylalanine, growth eventually becomes steady state or logarithmic. The logarithmic rate is inversely proportional to the extent of incorporation of this analogue or of p-fluorophenylalanine, and the projected maximal replacement is the same as that obtained asymptotically with the analogue alone. Thus, the toxicities in steady-state and non-steady-state growth are closely related. Moreover, it is proposed that single salient protein defects may determine the extent of growth rate reduction."}
{"id": "PMID:1096809", "title": "Empiric therapy for cancer patients: comparative study of ticarcillin-tobramycin, ticarcillin-cephalothin, and cephalothin-tobramycin.", "content": "Three combinations of antibiotics (cephalothin-tobramycin, cephalothin-ticarcillin, and ticarcillin-tobramycin) were administered empirically to 186 patients with cancer who were suspected of having a life-threatening infection. In approximately one-half of these patients, gram-negative infection was documented bacteriologically and consisted of septicemia in 50% of these patients. The three antimicrobial regimens were similarly effective and resulted in a favorable clinical response in approximately 55% of the patients. The administration of the cephalothin-tobramycin combination was associated with a significantly higher frequency of nephrotoxicity than that of the other two regimens.", "contents": "Empiric therapy for cancer patients: comparative study of ticarcillin-tobramycin, ticarcillin-cephalothin, and cephalothin-tobramycin. Three combinations of antibiotics (cephalothin-tobramycin, cephalothin-ticarcillin, and ticarcillin-tobramycin) were administered empirically to 186 patients with cancer who were suspected of having a life-threatening infection. In approximately one-half of these patients, gram-negative infection was documented bacteriologically and consisted of septicemia in 50% of these patients. The three antimicrobial regimens were similarly effective and resulted in a favorable clinical response in approximately 55% of the patients. The administration of the cephalothin-tobramycin combination was associated with a significantly higher frequency of nephrotoxicity than that of the other two regimens."}
{"id": "PMID:1096810", "title": "Treatment of anaerobic infections with metronidazole.", "content": "The results of treatment of 10 patients with anaerobic infections with metronidazole are presented. Six patients were cured, three showed initial good response but circumstances required a change to another drug, and one patient did not respond. The unique spectrum of the drug, its pharmacology, and limitations are discussed. The results indicate that further clinical trials to determine the efficacy of metronidazole in the treatment of anerobic infections are indicated.", "contents": "Treatment of anaerobic infections with metronidazole. The results of treatment of 10 patients with anaerobic infections with metronidazole are presented. Six patients were cured, three showed initial good response but circumstances required a change to another drug, and one patient did not respond. The unique spectrum of the drug, its pharmacology, and limitations are discussed. The results indicate that further clinical trials to determine the efficacy of metronidazole in the treatment of anerobic infections are indicated."}
{"id": "PMID:1096811", "title": "Antibacterial activity of cinoxacin in vitro.", "content": "Cinoxacin is a new synthetic compound similar chemically and in antimicrobial activity to oxolonic acid and nalidixic acid. It is most effective against Escherichia coli and Proteus mirabilis, but at concentrations expected in the urine it is inhibitory for all species of Enterobacteriaceae. Relative to nalidixic acid, cinoxacin has slightly greater inhibitory and bactericidal activity, less inoculum effect probably due to less heterogeneity in the susceptibility of bacterial cells, and less inhibition by high concentrations of serum protein. Both drugs are more active in an acid than an alkaline medium. Glucose can specifically antagonize the inhibitory effect against P. mirabilis. In urine the bactericidal rate and effect are decreased. Resistance to cinoxacin can be developed quickly by serial transfers in vitro. Some nonresistant organisms remained viable in bactericidal drug concentrations. The in vivo importance of the favorable features of cinoxacin must be determined by clinical trials.", "contents": "Antibacterial activity of cinoxacin in vitro. Cinoxacin is a new synthetic compound similar chemically and in antimicrobial activity to oxolonic acid and nalidixic acid. It is most effective against Escherichia coli and Proteus mirabilis, but at concentrations expected in the urine it is inhibitory for all species of Enterobacteriaceae. Relative to nalidixic acid, cinoxacin has slightly greater inhibitory and bactericidal activity, less inoculum effect probably due to less heterogeneity in the susceptibility of bacterial cells, and less inhibition by high concentrations of serum protein. Both drugs are more active in an acid than an alkaline medium. Glucose can specifically antagonize the inhibitory effect against P. mirabilis. In urine the bactericidal rate and effect are decreased. Resistance to cinoxacin can be developed quickly by serial transfers in vitro. Some nonresistant organisms remained viable in bactericidal drug concentrations. The in vivo importance of the favorable features of cinoxacin must be determined by clinical trials."}
{"id": "PMID:1096812", "title": "Scanning electron microscopy of Candida albicans after in vitro treatment with miconazole.", "content": "A study was made on the in vitro action of the antimycotic miconazole on Candida albicans yeast cells with scanning electron microscopy, and the effects were compared with those seen on the yeast cells by means of transmission electron microscopy. It was found that cells exposed to fungistatic and minimal fungicidal doses of miconazole (10(-7) M and 10(-6) M) presented rough surfaces and had multiple, desoriented buds and bud scars. Whereas in control cultures the cells were well separated, the treated ones formed small clusters of interconnected cells. After exposure to a fungicidal concentration (10(-4) M) of the drug, most of the remaining cells showed smooth surfaces and were covered with numerous vesicular structures probably representing cytoplasmic remnants derived from broken cells. This has been substantiated by the presence of abundant fragments of cell walls and confirmed by examination of similarly treated cultures in the transmission electron microscope. Moreover, the cells with an apparently intact surface when examined with scanning electron microscopy were shown with transmission electron microscopical examination to be completely necrotic inside.", "contents": "Scanning electron microscopy of Candida albicans after in vitro treatment with miconazole. A study was made on the in vitro action of the antimycotic miconazole on Candida albicans yeast cells with scanning electron microscopy, and the effects were compared with those seen on the yeast cells by means of transmission electron microscopy. It was found that cells exposed to fungistatic and minimal fungicidal doses of miconazole (10(-7) M and 10(-6) M) presented rough surfaces and had multiple, desoriented buds and bud scars. Whereas in control cultures the cells were well separated, the treated ones formed small clusters of interconnected cells. After exposure to a fungicidal concentration (10(-4) M) of the drug, most of the remaining cells showed smooth surfaces and were covered with numerous vesicular structures probably representing cytoplasmic remnants derived from broken cells. This has been substantiated by the presence of abundant fragments of cell walls and confirmed by examination of similarly treated cultures in the transmission electron microscope. Moreover, the cells with an apparently intact surface when examined with scanning electron microscopy were shown with transmission electron microscopical examination to be completely necrotic inside."}
{"id": "PMID:1096814", "title": "Pfizer selective enterococcus agar overlay method for the enumeration of fecal streptococci by membrane filtration.", "content": "The use of Pfizer selective enterococcus (PSE) agar with the membrane filter technique for the enumeration of fecal streptococci is limited due to the inability of the characteristic black precipitate, indicative of esculin hydrolysis, to diffuse from the medium through the membrane. A modification of the membrane filter technique that consisted of placing the membrane on PSE agar and overlaying it with tempered PSE agar was evaluated by comparing recovery, selectivity, and other parameters with M-enterococcus and KF-streptococcus agars, two selective media routinely used with the membrane filter technique for the enumeration of fecal streptococci in water and wastewater. No statistically significant differences could be demonstrated in the recovery capabilities of the three media. Inasmuch as the PSE overlay technique requires only 24 h of incubation as opposed to 48 h for the other two media, this modification may have some merit in water pollution monitoring programs.", "contents": "Pfizer selective enterococcus agar overlay method for the enumeration of fecal streptococci by membrane filtration. The use of Pfizer selective enterococcus (PSE) agar with the membrane filter technique for the enumeration of fecal streptococci is limited due to the inability of the characteristic black precipitate, indicative of esculin hydrolysis, to diffuse from the medium through the membrane. A modification of the membrane filter technique that consisted of placing the membrane on PSE agar and overlaying it with tempered PSE agar was evaluated by comparing recovery, selectivity, and other parameters with M-enterococcus and KF-streptococcus agars, two selective media routinely used with the membrane filter technique for the enumeration of fecal streptococci in water and wastewater. No statistically significant differences could be demonstrated in the recovery capabilities of the three media. Inasmuch as the PSE overlay technique requires only 24 h of incubation as opposed to 48 h for the other two media, this modification may have some merit in water pollution monitoring programs."}
{"id": "PMID:1096815", "title": "Occurrence of Klebisiella pneumoniae in surface waters.", "content": "The occurrence of Klebsiella pneumoniae in surface waters was not found to be ubiquitous. When it was isolated, Escherichia coli could also be found. The fecal coliform to fecal streptococci ratio suggest that its origin could be human, animal, or mixed sources.", "contents": "Occurrence of Klebisiella pneumoniae in surface waters. The occurrence of Klebsiella pneumoniae in surface waters was not found to be ubiquitous. When it was isolated, Escherichia coli could also be found. The fecal coliform to fecal streptococci ratio suggest that its origin could be human, animal, or mixed sources."}
{"id": "PMID:1096816", "title": "Inhibition of microbial growth by fatty amine catalysts from polyurethane foam test tube plugs.", "content": "When polyurethane foam test tube plugs are autoclaved, they release volatile fatty amines that inhibit the growth of some microorganisms. The chemical structures of these amines were determined by the use of a gas chromatograph-mass spectrometer. They are catalysts used to produce the foam. The problem of contaminating growth media with toxic substances released from polymeric materials is discussed.", "contents": "Inhibition of microbial growth by fatty amine catalysts from polyurethane foam test tube plugs. When polyurethane foam test tube plugs are autoclaved, they release volatile fatty amines that inhibit the growth of some microorganisms. The chemical structures of these amines were determined by the use of a gas chromatograph-mass spectrometer. They are catalysts used to produce the foam. The problem of contaminating growth media with toxic substances released from polymeric materials is discussed."}
{"id": "PMID:1096817", "title": "Isolation and characterization of Vibrio parahaemolyticus from Cape Cod soft-shell clams (Mya arenaria).", "content": "Vibrio parahaemolyticus was isolated from soft-shell clams (Mya arenaria) taken from 10 different clamming areas on Cape Cod, Mass., during July and August 1972. Direct plating on thiosulfate-citrate-bile salts-sucrose agar was found to be superior to either direct plating on Vanderzant modified salt starch agar or enrichment with Trypticase soy broth containing 7% salt for isolation from clam samples. Morphological and biochemical characteristics of 33 isolates from 30 samples generally conform to those described for this organism in the literature, except for the production of acid from sucrose, lactose, and sorbitol. Six of the isolates were hemolytic on human blood agar plates, whereas all showed a negative Kanagawa phenomenon. Twenty of the 33 isolates reacted with pooled antisera to the K antigen; 15 of these reacted with 9 different specific K antisera, leaving 5 untypable. Ten of these 15 reacted with 4 different O antisera.", "contents": "Isolation and characterization of Vibrio parahaemolyticus from Cape Cod soft-shell clams (Mya arenaria). Vibrio parahaemolyticus was isolated from soft-shell clams (Mya arenaria) taken from 10 different clamming areas on Cape Cod, Mass., during July and August 1972. Direct plating on thiosulfate-citrate-bile salts-sucrose agar was found to be superior to either direct plating on Vanderzant modified salt starch agar or enrichment with Trypticase soy broth containing 7% salt for isolation from clam samples. Morphological and biochemical characteristics of 33 isolates from 30 samples generally conform to those described for this organism in the literature, except for the production of acid from sucrose, lactose, and sorbitol. Six of the isolates were hemolytic on human blood agar plates, whereas all showed a negative Kanagawa phenomenon. Twenty of the 33 isolates reacted with pooled antisera to the K antigen; 15 of these reacted with 9 different specific K antisera, leaving 5 untypable. Ten of these 15 reacted with 4 different O antisera."}
{"id": "PMID:1096818", "title": "Qualitative and quantitative assay of trichothecin: a mycotoxin produced by Trichothecium roseum.", "content": "A method for quantitative determination of trichothecin in crude culture filtrates was presented. The method utilized an agar diffusion bioassay against Candida albicans, a colorimetric test involving a halochromatic reaction with sulfuric acid, and subsequent formation of blue color with methanol, and thin-layer chromatography of trichothecin and its dinitrophenylhydrazine derivative. A positive result in all three systems confirmed the presence of trichothecin. Quantitative results were generally in close agreement.", "contents": "Qualitative and quantitative assay of trichothecin: a mycotoxin produced by Trichothecium roseum. A method for quantitative determination of trichothecin in crude culture filtrates was presented. The method utilized an agar diffusion bioassay against Candida albicans, a colorimetric test involving a halochromatic reaction with sulfuric acid, and subsequent formation of blue color with methanol, and thin-layer chromatography of trichothecin and its dinitrophenylhydrazine derivative. A positive result in all three systems confirmed the presence of trichothecin. Quantitative results were generally in close agreement."}
{"id": "PMID:1096819", "title": "Effect of dichlorodifluoromethane on the appearance, viability, and integrity of Escherichia coli.", "content": "Cultures of Escherichia coli H52 were treated with liquid dichlorodifluoromethane (fluorocarbon-12 [f-12]) for 2 h at 22 C and then examined microscopically. Treated cells tended to clump, and their cytoplasms were generally less dense and less uniform in appearance than those of control cells. E. coli ML30 was exposed to f-12 at a concentration of 1.25 X saturation for times up to 1,200 min at 22 C. Cells were examined for changes in viability (plate count), permeability (as measured by exit of alpha-[14-C]methylglucoside or uptake of omicron-nitrophenyl-beta-D-galactopyranoside), release of compounds absorbing at 260 nm, and lysis (changes in absorbance at 420 nm). Large losses of alpha-methylglucoside and of percentage of viability occurred after brief exposure to f-12. Release of compounds absorbing at 260 nm occurred more slowly than the aforementioned events, possibly because these molecules are larger than alpha-methylglucoside. During 1,200-min exposure to f-12, the number of survivors decreased from 10-9 to 10-4 organisms/ml, the loss of compounds absorbing at 260 nm amounted to 50 percent, and 32 percent lysis occurred. Most of these changes occurred during the first 300 min of treatment. Loss of alpha-methylglucoside was almost complete after 1-min exposure to f-12. These results suggest that death of the cell involves several stages, with a change of permeability, occurring first, followed by leakage of compounds of increasing size and, finally, lysis.", "contents": "Effect of dichlorodifluoromethane on the appearance, viability, and integrity of Escherichia coli. Cultures of Escherichia coli H52 were treated with liquid dichlorodifluoromethane (fluorocarbon-12 [f-12]) for 2 h at 22 C and then examined microscopically. Treated cells tended to clump, and their cytoplasms were generally less dense and less uniform in appearance than those of control cells. E. coli ML30 was exposed to f-12 at a concentration of 1.25 X saturation for times up to 1,200 min at 22 C. Cells were examined for changes in viability (plate count), permeability (as measured by exit of alpha-[14-C]methylglucoside or uptake of omicron-nitrophenyl-beta-D-galactopyranoside), release of compounds absorbing at 260 nm, and lysis (changes in absorbance at 420 nm). Large losses of alpha-methylglucoside and of percentage of viability occurred after brief exposure to f-12. Release of compounds absorbing at 260 nm occurred more slowly than the aforementioned events, possibly because these molecules are larger than alpha-methylglucoside. During 1,200-min exposure to f-12, the number of survivors decreased from 10-9 to 10-4 organisms/ml, the loss of compounds absorbing at 260 nm amounted to 50 percent, and 32 percent lysis occurred. Most of these changes occurred during the first 300 min of treatment. Loss of alpha-methylglucoside was almost complete after 1-min exposure to f-12. These results suggest that death of the cell involves several stages, with a change of permeability, occurring first, followed by leakage of compounds of increasing size and, finally, lysis."}
{"id": "PMID:1096820", "title": "Sequential growth of bacteria on crude oil.", "content": "By modification of the enrichment culture procedure three bacterial strains capable of degrading crude oil in sea water were isolated in pure culture, UP-2, UP-3, and UP-4. Strain UP-2 appears to be highly specialized for growth on crude oil in sea water since it showed strong preference for oil or oil degradation products as substrates for growth, converted 66% of the oil into a form no longer extractable by organic solvents, quantitatively degraded the paraffinic fraction (gas chromatographic analysis), emulsified the oil during exponential growth, and produced 1.6 x 10-8 cells per mg of oil. After exhaustive growth of UP-2 on crude oil the residual oil supported the growth of UP-3 and UP-4, but not a previously isolated oil-degrading bacterium, RAG-1. Strains UP-2, UP-3, and UP-4 grew on RAG-1-degraded oil (specifically depleted of n-alkanes). The growth of UP-3 and UP-4 on UP-2 and RAG-1-degraded oil resulted in the production of new paraffinic compounds as revealed by gas chromatography. When the four strains were grown either together in a mixed culture or sequentially, there was over 75% oil conversion. By plating on selective media, growth of the individual strains was measured kinetically in the reconstituted mixed culture, revealing competition for common growth substances (UP-2 and RAG-1), emhanced die-off (UP-2), and stabilization (UP-4) during the stationary phase.", "contents": "Sequential growth of bacteria on crude oil. By modification of the enrichment culture procedure three bacterial strains capable of degrading crude oil in sea water were isolated in pure culture, UP-2, UP-3, and UP-4. Strain UP-2 appears to be highly specialized for growth on crude oil in sea water since it showed strong preference for oil or oil degradation products as substrates for growth, converted 66% of the oil into a form no longer extractable by organic solvents, quantitatively degraded the paraffinic fraction (gas chromatographic analysis), emulsified the oil during exponential growth, and produced 1.6 x 10-8 cells per mg of oil. After exhaustive growth of UP-2 on crude oil the residual oil supported the growth of UP-3 and UP-4, but not a previously isolated oil-degrading bacterium, RAG-1. Strains UP-2, UP-3, and UP-4 grew on RAG-1-degraded oil (specifically depleted of n-alkanes). The growth of UP-3 and UP-4 on UP-2 and RAG-1-degraded oil resulted in the production of new paraffinic compounds as revealed by gas chromatography. When the four strains were grown either together in a mixed culture or sequentially, there was over 75% oil conversion. By plating on selective media, growth of the individual strains was measured kinetically in the reconstituted mixed culture, revealing competition for common growth substances (UP-2 and RAG-1), emhanced die-off (UP-2), and stabilization (UP-4) during the stationary phase."}
{"id": "PMID:1096821", "title": "Production of cellulose microfibrils by Rhizobium.", "content": "Electron microscope examination of Rhizobium spp. revealed microfibrils produced by flocculating strains but not by nonflocculating strains. The microfibrils from R. trifolii (NA30) were isolated and identified as cellulose by enzymatic, X-ray diffraction, and infrared spectral analyses. Both infective and noninfective strains of R. trifolii flocculated and produced microfibrils. More infection threads were observed in clover root hairs growing in the presence of flocs in comparison with root hairs where single bacterial cells predominated.", "contents": "Production of cellulose microfibrils by Rhizobium. Electron microscope examination of Rhizobium spp. revealed microfibrils produced by flocculating strains but not by nonflocculating strains. The microfibrils from R. trifolii (NA30) were isolated and identified as cellulose by enzymatic, X-ray diffraction, and infrared spectral analyses. Both infective and noninfective strains of R. trifolii flocculated and produced microfibrils. More infection threads were observed in clover root hairs growing in the presence of flocs in comparison with root hairs where single bacterial cells predominated."}
{"id": "PMID:1096822", "title": "Fluorescent-antibody study of natural finger-like zoogloeae.", "content": "Fluorescent-antibody techniques using Zoogloea ramigera 106 antiserum were used to study fresh activated sludge flocs and finger-like zoogloeae in the microbial film that developed over stored samples of activated sludge. Few cells in fresh activated sludge reacted positively with the fluorescein-labeled antiserum. Finger-like zoogloeae containing reactive cells were readily observed in the microbial film layer over stored activated sludge. Certain of the naturel finger-like projections were entirely composed of cells that reacted positively to the labeled Z. ramigera 106 antiserum, whereas other projections were devoid of reactive cells.", "contents": "Fluorescent-antibody study of natural finger-like zoogloeae. Fluorescent-antibody techniques using Zoogloea ramigera 106 antiserum were used to study fresh activated sludge flocs and finger-like zoogloeae in the microbial film that developed over stored samples of activated sludge. Few cells in fresh activated sludge reacted positively with the fluorescein-labeled antiserum. Finger-like zoogloeae containing reactive cells were readily observed in the microbial film layer over stored activated sludge. Certain of the naturel finger-like projections were entirely composed of cells that reacted positively to the labeled Z. ramigera 106 antiserum, whereas other projections were devoid of reactive cells."}
{"id": "PMID:1096823", "title": "Effect of aflatoxin on phagocytosis of Aspergillus fumigatus spores by rabbit alveolar macrophages.", "content": "Rabbits were given dialy doses of aflatoxin B1 equivalents of 0.03, 0.05, 0.07, and 0.09 mg for a 2-week period. Macrophages were harvested at the end of the experimental period, and in vitro phagocytosis experiments were conducted using Aspergillus fumigatus spores as ingestion particles. Alveolar macrophages from rabbits given the above doses of aflatoxin had reduced phagocytic activity when compared with macrophages from control rabbits. Incorporation of serum from the aflatoxin-treated rabbits in the in vitro culture system resulted in less stimulation of phagocytosis by macrophages from control rabbits when compared with the same system incorporating control serum. Stimulation of phagocytosis by macrophages was least when both serum and macrophages from aflatoxin-treated rabbits were used in the in vitro system.", "contents": "Effect of aflatoxin on phagocytosis of Aspergillus fumigatus spores by rabbit alveolar macrophages. Rabbits were given dialy doses of aflatoxin B1 equivalents of 0.03, 0.05, 0.07, and 0.09 mg for a 2-week period. Macrophages were harvested at the end of the experimental period, and in vitro phagocytosis experiments were conducted using Aspergillus fumigatus spores as ingestion particles. Alveolar macrophages from rabbits given the above doses of aflatoxin had reduced phagocytic activity when compared with macrophages from control rabbits. Incorporation of serum from the aflatoxin-treated rabbits in the in vitro culture system resulted in less stimulation of phagocytosis by macrophages from control rabbits when compared with the same system incorporating control serum. Stimulation of phagocytosis by macrophages was least when both serum and macrophages from aflatoxin-treated rabbits were used in the in vitro system."}
{"id": "PMID:1096824", "title": "Microbiological characteristics of Dungeness crab (Cancer magister).", "content": "Aerobic, heterotropic microorganisms of Dungeness crab (Cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. Each microbial isolate was then identified to the genus level employing the revised replica plating procedure. Microbial groups most commonly isolated from crab meat were, in the order of predominance, Moraxella, Pseudomonas, Acinetobacter, Arthrobacter, Micrococcus, Flavobacterium-Cytophaga, and Bacillus sp. Proteus, Staphylococcus, yeasts, Vibrio, and Lactobacillus sp. were found less frequetly in some samples. Distribution patterns of microbial flora in crab meat revealed the presence of three classes of microorganisms. Microorganisms that originated from the raw crab and gained predominance by growth during refrigerated storage were Moraxella, Pseudomonas, Acinetobacter, and Flavobacterium-Cytophaga sp. Those that originated from the crab but did not grow in meat were Arthrobacter and Bacillus sp. Micrococcus, Staphylococcus, and Proteus sp. were introduced during processing, but they did not grow in the refrigerated crab meat.", "contents": "Microbiological characteristics of Dungeness crab (Cancer magister). Aerobic, heterotropic microorganisms of Dungeness crab (Cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. Each microbial isolate was then identified to the genus level employing the revised replica plating procedure. Microbial groups most commonly isolated from crab meat were, in the order of predominance, Moraxella, Pseudomonas, Acinetobacter, Arthrobacter, Micrococcus, Flavobacterium-Cytophaga, and Bacillus sp. Proteus, Staphylococcus, yeasts, Vibrio, and Lactobacillus sp. were found less frequetly in some samples. Distribution patterns of microbial flora in crab meat revealed the presence of three classes of microorganisms. Microorganisms that originated from the raw crab and gained predominance by growth during refrigerated storage were Moraxella, Pseudomonas, Acinetobacter, and Flavobacterium-Cytophaga sp. Those that originated from the crab but did not grow in meat were Arthrobacter and Bacillus sp. Micrococcus, Staphylococcus, and Proteus sp. were introduced during processing, but they did not grow in the refrigerated crab meat."}
{"id": "PMID:1096825", "title": "Application of the deoxyribonucleic acid/ribonucleic acid hybridization technique in Bdellovibrio as a model for studying ribonucleic acid turnover in host-parasite systems.", "content": "The kinetics of host ribonucleic acid (RNA) degradation and its resynthesis into Bdellovibrio-specific polyribonucleotides has been studied. The kinetics of RNA turnover was followed during a one-step synchronous growth cycle of Bdellovibrio growing within 32-po4-Labeled Escherichia coli host cells. The species of labeled RNA present at any given time was ascertained through the specificity of the deoxyribonuclei acid (DNA)/RNA hybridization technique. At nearsaturating levels of RNA and at zero time, 7% of the host DNA sequences and only 0.04% of the Bdellovibrio DNA became hybridized with 32-P-labeled host cell RNA (greater than 99% host specific). At the end of the burst, 98% of the labeled RNA sequences were specific for Bdellovibrio DNA. About 74% of the initial labeled host cell RNA became turned over into Bdellovibrio-specific sequences. We provide data indicating that host cell ribosomal RNA is assimilated by Bdellovibri. Degradation of host cell RNA occurs in a gradual fashion over most of the Bdellovibrio developmental growth cycle. This application of the DNA/RNA hybridization technique and its general concept should be of value in elucidating the kinetics of nucleic acid turnover in other types of host-parasite systems.", "contents": "Application of the deoxyribonucleic acid/ribonucleic acid hybridization technique in Bdellovibrio as a model for studying ribonucleic acid turnover in host-parasite systems. The kinetics of host ribonucleic acid (RNA) degradation and its resynthesis into Bdellovibrio-specific polyribonucleotides has been studied. The kinetics of RNA turnover was followed during a one-step synchronous growth cycle of Bdellovibrio growing within 32-po4-Labeled Escherichia coli host cells. The species of labeled RNA present at any given time was ascertained through the specificity of the deoxyribonuclei acid (DNA)/RNA hybridization technique. At nearsaturating levels of RNA and at zero time, 7% of the host DNA sequences and only 0.04% of the Bdellovibrio DNA became hybridized with 32-P-labeled host cell RNA (greater than 99% host specific). At the end of the burst, 98% of the labeled RNA sequences were specific for Bdellovibrio DNA. About 74% of the initial labeled host cell RNA became turned over into Bdellovibrio-specific sequences. We provide data indicating that host cell ribosomal RNA is assimilated by Bdellovibri. Degradation of host cell RNA occurs in a gradual fashion over most of the Bdellovibrio developmental growth cycle. This application of the DNA/RNA hybridization technique and its general concept should be of value in elucidating the kinetics of nucleic acid turnover in other types of host-parasite systems."}
{"id": "PMID:1096827", "title": "Use of immunofluorescence staining in kerion.", "content": "We describe two cases of kerion caused by Trichophyton rebrum and Microsporum gypseum. In the histological sections, a PAS stain revealed a small number of hypha-like rods and spore-like round bodies in the dermis of patient 1. Except for some clusters of PAS-positive materials, no definite fungal elements were observed in the dermis of patient 1, whereas a considerable number of hyphae were demonstrated within a hair shaft. Immunofluorescence staining, using fluorescein-labeled T mentagrophytes antiserum, disclosed the presence of fungal antigens, not only within a hair shaft, but also in the inflammatory infiltrate in the dermis of both patients.", "contents": "Use of immunofluorescence staining in kerion. We describe two cases of kerion caused by Trichophyton rebrum and Microsporum gypseum. In the histological sections, a PAS stain revealed a small number of hypha-like rods and spore-like round bodies in the dermis of patient 1. Except for some clusters of PAS-positive materials, no definite fungal elements were observed in the dermis of patient 1, whereas a considerable number of hyphae were demonstrated within a hair shaft. Immunofluorescence staining, using fluorescein-labeled T mentagrophytes antiserum, disclosed the presence of fungal antigens, not only within a hair shaft, but also in the inflammatory infiltrate in the dermis of both patients."}
{"id": "PMID:1096829", "title": "Agar in the management of hyperbilirubinaemia in the premature baby.", "content": "Eighty low birthweight infants (1500 to 2500 g) were randomly assigned to a group given oral agar and a control group. The study was first performed in 24 agar-fed babies and 24 control babies. The treated infants received a daily total amount of 600 mg/kg of agar beginning at 12 hours of age, for 7 days. In the later stage of the trial, 16 agar-fed babies and 16 control babies were investigated. The total daily amount of agar was raised to 700 mg/kg, beginning at 6 hours of age for 7 days. No complications were observed with the administration of agar. Serum bilirubin levels were not significantly lower in the agar-fed infants. We conclude that agar supplemented feeding is not indicated in the management of hyperbilirubinaemia in low birthweight newborn infants.", "contents": "Agar in the management of hyperbilirubinaemia in the premature baby. Eighty low birthweight infants (1500 to 2500 g) were randomly assigned to a group given oral agar and a control group. The study was first performed in 24 agar-fed babies and 24 control babies. The treated infants received a daily total amount of 600 mg/kg of agar beginning at 12 hours of age, for 7 days. In the later stage of the trial, 16 agar-fed babies and 16 control babies were investigated. The total daily amount of agar was raised to 700 mg/kg, beginning at 6 hours of age for 7 days. No complications were observed with the administration of agar. Serum bilirubin levels were not significantly lower in the agar-fed infants. We conclude that agar supplemented feeding is not indicated in the management of hyperbilirubinaemia in low birthweight newborn infants."}
{"id": "PMID:1096830", "title": "Familial opsonization defect associated with fatal infantile dermatitis, infections, and histiocytosis.", "content": "Members of four generations of a family had a defect of serum opsonization for yeast phagocytosis consistent with dominant inheritance. 2 were healthy, one had chronic osteomyelitis, and the fourth developed a fatal illness in infancy characterized by exfoliative dermatitis, diarrhoea, multiple bacterial infections, and failure to thrive, which resembled the two prevously reported cases with this opsonization defect. At necropsy the infant also had lymphoid depletion, which was possibly secondary, and massive histiocytic infiltration.", "contents": "Familial opsonization defect associated with fatal infantile dermatitis, infections, and histiocytosis. Members of four generations of a family had a defect of serum opsonization for yeast phagocytosis consistent with dominant inheritance. 2 were healthy, one had chronic osteomyelitis, and the fourth developed a fatal illness in infancy characterized by exfoliative dermatitis, diarrhoea, multiple bacterial infections, and failure to thrive, which resembled the two prevously reported cases with this opsonization defect. At necropsy the infant also had lymphoid depletion, which was possibly secondary, and massive histiocytic infiltration."}
{"id": "PMID:1096831", "title": "\"Added lactose\" and \"added sucrose\" cow's milk formulae in nutrition of low birthweight babies.", "content": "During the manufacture of dried milks for infant feeding the composition of cow's milk may be modified by the addition of extra carbohydrate powder to lower the relative proportions of protein and minerals, and in practice various carbohydrates are used in a largely empirical manner. In other circumstances it is known that the quality of dietary carbohydrate affects intestinal tolerance, deposition of body fat (in rats), and concentrations of plasma lipids (in man). Therefore, in this study the effects of feeding newborn infants on added lactose formula and added sucrose formula have been investigated. 29 low birthweight babies were observed throughout the first 3 months of life. The added carbohydrate achieved a satisfactory composition in terms of mineral and protein concentration of the reconstituted milk, but the \"added lactose\" group experienced more diarrhoea and a greater degree of metabolic acidosis during the first week of life. The added lactose group was slightly fatter and the plasma triglyceride concentration slightly higher than in the \"added sucrose\" group. Despite teleological evidence in favour of lactose, we found no objective contraindication to the addition of sucrose to cow's milk in the manufacture of infant feeding formulae. Both milks contained only small quantities of linoleic acid and the polyunsaturated fatty acid content of the plasma and adipose tissue lipids fell to low levels, but no clinical evidence of \"essential fatty acid deficiency\" was found.", "contents": "\"Added lactose\" and \"added sucrose\" cow's milk formulae in nutrition of low birthweight babies. During the manufacture of dried milks for infant feeding the composition of cow's milk may be modified by the addition of extra carbohydrate powder to lower the relative proportions of protein and minerals, and in practice various carbohydrates are used in a largely empirical manner. In other circumstances it is known that the quality of dietary carbohydrate affects intestinal tolerance, deposition of body fat (in rats), and concentrations of plasma lipids (in man). Therefore, in this study the effects of feeding newborn infants on added lactose formula and added sucrose formula have been investigated. 29 low birthweight babies were observed throughout the first 3 months of life. The added carbohydrate achieved a satisfactory composition in terms of mineral and protein concentration of the reconstituted milk, but the \"added lactose\" group experienced more diarrhoea and a greater degree of metabolic acidosis during the first week of life. The added lactose group was slightly fatter and the plasma triglyceride concentration slightly higher than in the \"added sucrose\" group. Despite teleological evidence in favour of lactose, we found no objective contraindication to the addition of sucrose to cow's milk in the manufacture of infant feeding formulae. Both milks contained only small quantities of linoleic acid and the polyunsaturated fatty acid content of the plasma and adipose tissue lipids fell to low levels, but no clinical evidence of \"essential fatty acid deficiency\" was found."}
{"id": "PMID:1096832", "title": "Autoantibodies in childhood connective tissue diseases and in normal children.", "content": "The prevalence of nine serum autoantibodies has been studied in 117 children with various connective tissue disorders and in 134 normal controls. In juvenile rheumatoid arthritis rheumatoid factor was present in 5%, and antinuclear factor in 4%, compared with an incidence of 4% and 0% respectively in controls. In Henoch-Sch\u00f6nlein purpura there was little evidence of associated autoimmune disorder. Gastric parietal cell and thyroid microsomal antibodies were found in 9% and 10% of our control population, but the significance of this is not clear. It is concluded that in children the presence or absence of autoantibodies as diagnostic criteria should be interpreted with the greatest caution.", "contents": "Autoantibodies in childhood connective tissue diseases and in normal children. The prevalence of nine serum autoantibodies has been studied in 117 children with various connective tissue disorders and in 134 normal controls. In juvenile rheumatoid arthritis rheumatoid factor was present in 5%, and antinuclear factor in 4%, compared with an incidence of 4% and 0% respectively in controls. In Henoch-Sch\u00f6nlein purpura there was little evidence of associated autoimmune disorder. Gastric parietal cell and thyroid microsomal antibodies were found in 9% and 10% of our control population, but the significance of this is not clear. It is concluded that in children the presence or absence of autoantibodies as diagnostic criteria should be interpreted with the greatest caution."}
{"id": "PMID:1096833", "title": "Isosorbide in treatment of infantile hydrocephalus.", "content": "This paper reports the experiences of the second clinical trial in the use of isosorbide in the treatment of 34 selected cases of infantile hydrocephalus of all types. Subject to careful biochemical monitoring of serum electrolyte, urea, and acid-base balance, treatment with 2 g/kg body weight 6-hourly is safe. Side effects are immediately eliminated by interrupting therapy. With lower dosage, prolonged maintenance therapy was possible, for as long as 11 months, without side effects and with need for much less frequent biochemical monitoring. Isosorbide effectively prevented the need for shunt therapy in 10 of 34 patients, including 3 infants with uncomplicated congenital hydrocephalus of moderate degree and infants whose hydrocephalus was associated with spina bifida and whose cerebral mantle was between 20 to 25 mm. In posthaemorrhagic and postmeningitic hydrocephalus valuable time was gained before shunt therapy until the infant and his CSF were fit for operation.", "contents": "Isosorbide in treatment of infantile hydrocephalus. This paper reports the experiences of the second clinical trial in the use of isosorbide in the treatment of 34 selected cases of infantile hydrocephalus of all types. Subject to careful biochemical monitoring of serum electrolyte, urea, and acid-base balance, treatment with 2 g/kg body weight 6-hourly is safe. Side effects are immediately eliminated by interrupting therapy. With lower dosage, prolonged maintenance therapy was possible, for as long as 11 months, without side effects and with need for much less frequent biochemical monitoring. Isosorbide effectively prevented the need for shunt therapy in 10 of 34 patients, including 3 infants with uncomplicated congenital hydrocephalus of moderate degree and infants whose hydrocephalus was associated with spina bifida and whose cerebral mantle was between 20 to 25 mm. In posthaemorrhagic and postmeningitic hydrocephalus valuable time was gained before shunt therapy until the infant and his CSF were fit for operation."}
{"id": "PMID:1096834", "title": "Pneumothorax in the newborn. Changing pattern.", "content": "The clinical course of pneumothorax and its allied conditions was studied in 34 newborn infants who presented over a 2 1/2-year period. We found an overall incidence of 3/1000 live births. 11 term infants without obvious pulmonary pathology presented early (9 within minutes of birth); 6 of these had aspirated meconium or blood. The remaining 23 were preterm infants with hyaline membrane disease (HMD) and accounted for 68% of the infants in this series. In contrast, they presented late (mean 45 hours) and 16 were on continuous distending pressure (CDP) or intermittent positive pressure ventilation (IPPV) at the onset of pneumothorax. 15% of all infants with HMD who required CDP/IPPV developed pneumothorax; this increased incidence was most evident in infants who received CDP only. All except 2 of the 11 term infants in the first group were managed conservatively and all survived. Wehn pneumothorax occurred as a complication of HMD in preterm infants, 14 of the 16 infants required intrapleural drainage. Persistence or recurrence of pneumothorax occurred in 9 infants, 7 of whom were receiving CDP/IPPV at the time. Lung expansion was affected only after replacement with a patent chest drain through the same incision or insertion of a second drain on the same side of the chest. All 5 deaths occurred in the group of preterm infants with HMD. 3 resulted directly form respiratory failure due to severe HMD complicated by pneumothorax. We emphasize the increasing importance of pneumothorax as a complication of HMD in preterm infants, particularly in those receiving CDP. Successful management depends on prompt diagnosis and treatment of pneumothorax, which may occur as unexplained sudden deterioration at any time during the course of illness in this group of high risk infants.", "contents": "Pneumothorax in the newborn. Changing pattern. The clinical course of pneumothorax and its allied conditions was studied in 34 newborn infants who presented over a 2 1/2-year period. We found an overall incidence of 3/1000 live births. 11 term infants without obvious pulmonary pathology presented early (9 within minutes of birth); 6 of these had aspirated meconium or blood. The remaining 23 were preterm infants with hyaline membrane disease (HMD) and accounted for 68% of the infants in this series. In contrast, they presented late (mean 45 hours) and 16 were on continuous distending pressure (CDP) or intermittent positive pressure ventilation (IPPV) at the onset of pneumothorax. 15% of all infants with HMD who required CDP/IPPV developed pneumothorax; this increased incidence was most evident in infants who received CDP only. All except 2 of the 11 term infants in the first group were managed conservatively and all survived. Wehn pneumothorax occurred as a complication of HMD in preterm infants, 14 of the 16 infants required intrapleural drainage. Persistence or recurrence of pneumothorax occurred in 9 infants, 7 of whom were receiving CDP/IPPV at the time. Lung expansion was affected only after replacement with a patent chest drain through the same incision or insertion of a second drain on the same side of the chest. All 5 deaths occurred in the group of preterm infants with HMD. 3 resulted directly form respiratory failure due to severe HMD complicated by pneumothorax. We emphasize the increasing importance of pneumothorax as a complication of HMD in preterm infants, particularly in those receiving CDP. Successful management depends on prompt diagnosis and treatment of pneumothorax, which may occur as unexplained sudden deterioration at any time during the course of illness in this group of high risk infants."}
{"id": "PMID:1096835", "title": "Prevalence of atopy and exercise-induced bronchial lability in relatives of patients with cystic fibrosis.", "content": "Skin tests and exercise tests were performed on children with cystic fibrosis (CF) and first-degree relatives of CF children. Positive skin tests were found in 56% of patients and 19% of relatives, but the prevalence of atopy in CF patients bore no relationship to its prevalence in their relatives. Increased 0ronchial lability, mainly due to bronchoconstriction, was present in 54% of patients and 27% of relatives. The results showed that increased prevalence of bronchial lability, but not atopy, is found in first-degree relatives of CF children, and that the abnormal lability and atopy found in CF children does not indicate a familial allergic background.", "contents": "Prevalence of atopy and exercise-induced bronchial lability in relatives of patients with cystic fibrosis. Skin tests and exercise tests were performed on children with cystic fibrosis (CF) and first-degree relatives of CF children. Positive skin tests were found in 56% of patients and 19% of relatives, but the prevalence of atopy in CF patients bore no relationship to its prevalence in their relatives. Increased 0ronchial lability, mainly due to bronchoconstriction, was present in 54% of patients and 27% of relatives. The results showed that increased prevalence of bronchial lability, but not atopy, is found in first-degree relatives of CF children, and that the abnormal lability and atopy found in CF children does not indicate a familial allergic background."}
{"id": "PMID:1096837", "title": "The removable buried retention suture: a new technique.", "content": "Through-and-through retention sutures are inherently inefficient and prone to produce skin and subcutaneous damage. Most surgeons are reluctant to permanently bury non-absorbable retention sutures. This new technique uses tube conduits to bring the retention sutures from deep fascial level to above the skin-surface, where they are available for removal. Thus, the sutures do not cross the skin and subcutaneous tissue, and they exert maximal efficiency at the deep fascial layers. This method has been used in over 60 cases without deep-wound separation, and complications have been minimal.", "contents": "The removable buried retention suture: a new technique. Through-and-through retention sutures are inherently inefficient and prone to produce skin and subcutaneous damage. Most surgeons are reluctant to permanently bury non-absorbable retention sutures. This new technique uses tube conduits to bring the retention sutures from deep fascial level to above the skin-surface, where they are available for removal. Thus, the sutures do not cross the skin and subcutaneous tissue, and they exert maximal efficiency at the deep fascial layers. This method has been used in over 60 cases without deep-wound separation, and complications have been minimal."}
{"id": "PMID:1096838", "title": "Central nervous systen alpha-adrenergic mechanisms and cardiovascular regulation in rats.", "content": "Noradrenaline (NA) induced a decrease in blood pressure and heart rate when injected into specific areas in either the medulla oblongata or the hypothalamus. In the medulla the area of the nucleus tractus solitarius was specifically sensitive to NA; in the hypothalamus depressor effects were obtained only after NA injections into the anterior hypothalamic/preoptic region. The cardiovascular effects induced by NA (3-40 nmol) in these areas consisted of an immediate decrease in both arterial pressure and heart rate. Size and duration of these effects depended upon the dose of NA injected. Alpha-methylNA (5-15 nmol) induced a long lasting decrease in blood pressure and heart rate when injected into the anterior hypothalamic/preoptic region. These data are discussed in view of the existence of at least two sites within the central nervous system (CNS) from which interference with noradrenergic mechanisms can cause changes in the cardiovascular system.", "contents": "Central nervous systen alpha-adrenergic mechanisms and cardiovascular regulation in rats. Noradrenaline (NA) induced a decrease in blood pressure and heart rate when injected into specific areas in either the medulla oblongata or the hypothalamus. In the medulla the area of the nucleus tractus solitarius was specifically sensitive to NA; in the hypothalamus depressor effects were obtained only after NA injections into the anterior hypothalamic/preoptic region. The cardiovascular effects induced by NA (3-40 nmol) in these areas consisted of an immediate decrease in both arterial pressure and heart rate. Size and duration of these effects depended upon the dose of NA injected. Alpha-methylNA (5-15 nmol) induced a long lasting decrease in blood pressure and heart rate when injected into the anterior hypothalamic/preoptic region. These data are discussed in view of the existence of at least two sites within the central nervous system (CNS) from which interference with noradrenergic mechanisms can cause changes in the cardiovascular system."}
{"id": "PMID:1096840", "title": "Treatment of fungal infections with flucytosine.", "content": "Treatment with flucytosine of 20 patients with fungal infections gave favorable results in four patients with crytococcal infections, two of four patients with disseminated candidiasis, eight of ten patients with urinary tract infections due to Candida albicans and Torulopsis glabrata, and tow of three patients with miscellaneous infections due to Calbicans. Two patients with crytococcal meningitis and altered host resistance and one patient with an aorto femoral graft infection due to C albicans were treated with flucytosine and smphotericin B. The infection was eradicated in one of the patients with meningitis, and cultures from an infected arterial graft became negative. Adverse side effects of flucytosine included mild leukopenia and thrombocytopenia, a transient increase in alkaline phosphatase and glutamic oxaloacetic transaminase, and nausea and diarrhea.", "contents": "Treatment of fungal infections with flucytosine. Treatment with flucytosine of 20 patients with fungal infections gave favorable results in four patients with crytococcal infections, two of four patients with disseminated candidiasis, eight of ten patients with urinary tract infections due to Candida albicans and Torulopsis glabrata, and tow of three patients with miscellaneous infections due to Calbicans. Two patients with crytococcal meningitis and altered host resistance and one patient with an aorto femoral graft infection due to C albicans were treated with flucytosine and smphotericin B. The infection was eradicated in one of the patients with meningitis, and cultures from an infected arterial graft became negative. Adverse side effects of flucytosine included mild leukopenia and thrombocytopenia, a transient increase in alkaline phosphatase and glutamic oxaloacetic transaminase, and nausea and diarrhea."}
{"id": "PMID:1096841", "title": "Malignant hypertension. Recovery of kidney function after renal allograft failure.", "content": "A 28-year old patient with malignant hypertension developed endstage renal failure necessitating chronic maintenance hemodialysis for seven months and transplantation of a renal allograft that was rejected within a month. After this, with continued control of the hypertension, the patient's own kidneys gradually resumed function and achieved anendogenous creatinine clearance of 30 ml/min. She has not required further dialysis for more than nine months. Recovery of sufficient renal function to maintain homeostasis, one year after onset of uremia, indicates the prolonged antihypertensive treatment period that may be necessary to reverse the renal arteriolar changes associated with malignant hypertension. This experience further underlines the necessity of moderation in the use of nephrectomy in the management of severe hypertension.", "contents": "Malignant hypertension. Recovery of kidney function after renal allograft failure. A 28-year old patient with malignant hypertension developed endstage renal failure necessitating chronic maintenance hemodialysis for seven months and transplantation of a renal allograft that was rejected within a month. After this, with continued control of the hypertension, the patient's own kidneys gradually resumed function and achieved anendogenous creatinine clearance of 30 ml/min. She has not required further dialysis for more than nine months. Recovery of sufficient renal function to maintain homeostasis, one year after onset of uremia, indicates the prolonged antihypertensive treatment period that may be necessary to reverse the renal arteriolar changes associated with malignant hypertension. This experience further underlines the necessity of moderation in the use of nephrectomy in the management of severe hypertension."}
{"id": "PMID:1096843", "title": "Effect of edeine on primary immunologic response in mice.", "content": "The effect of edeine on primary immune response against sheep erythrocytes in mice was studied. The response intensity was evaluated on the cellular and humoral level. The results showed significant decrease in the number of spleen cells capable of synthesizing hemolysins, and the decrease in serum level of these antibodies.", "contents": "Effect of edeine on primary immunologic response in mice. The effect of edeine on primary immune response against sheep erythrocytes in mice was studied. The response intensity was evaluated on the cellular and humoral level. The results showed significant decrease in the number of spleen cells capable of synthesizing hemolysins, and the decrease in serum level of these antibodies."}
{"id": "PMID:1096844", "title": "Adsorption of pneumocins and aerocins on bacterial cells.", "content": "Adsorption of aerocins 1a-E26, 1b-E47, 1b-E43, 2-E26, 2-E47 and pneumocins A-114 and A-902 was found to be dependent on the presence of specific receptors on the surface of sensitive bacteria. Resistant mutants, selected by these bacteriocins, were incapable of adsorption owing to loss or altered structure of receptors. Maximal numbers of Killing Units of pneumocins and aerocins adsorbed by one bacterial cell of sensitive strains were determined. Kinetics of adsorption and degree of killing of bacteria in relation to concentration of bacteriocins were studied. The lethal effect on bacteria was of the \"single hit\" type, i. e. one particle of aerocin respectively pneumocin killed one bacterial cell.", "contents": "Adsorption of pneumocins and aerocins on bacterial cells. Adsorption of aerocins 1a-E26, 1b-E47, 1b-E43, 2-E26, 2-E47 and pneumocins A-114 and A-902 was found to be dependent on the presence of specific receptors on the surface of sensitive bacteria. Resistant mutants, selected by these bacteriocins, were incapable of adsorption owing to loss or altered structure of receptors. Maximal numbers of Killing Units of pneumocins and aerocins adsorbed by one bacterial cell of sensitive strains were determined. Kinetics of adsorption and degree of killing of bacteria in relation to concentration of bacteriocins were studied. The lethal effect on bacteria was of the \"single hit\" type, i. e. one particle of aerocin respectively pneumocin killed one bacterial cell."}
{"id": "PMID:1096847", "title": "Variability of effect of positive end expiratory pressure.", "content": "Mechanical ventilation with positive end expiratory pressure (PEEP) has been reported to produce prompt improvement in oxygeneration when used to treat acute respiratory failure. Reports of the effect of PEEP on cardiac output have been conflicting. We studied 14 patients and found that, although mean values of arterial oxygen tension (PaO2) increased as PEEP increased, on eight occasions in the acute studies and three in the elective studies, it fell as PEEP was increased. On five occasions the fall in PaO2 was associated with an increased shunt. Arterial pressure did not, on the average, change at any level of PEEP, but in 13 of 30 measurements it fell by more than 20% and necessitated curtailment of the study at blood pressure levels of less than 50 torr. Since these effects of PEEP cannot be predicted, careful patient monitoring is essential when ventilation with PEEP is utilized.", "contents": "Variability of effect of positive end expiratory pressure. Mechanical ventilation with positive end expiratory pressure (PEEP) has been reported to produce prompt improvement in oxygeneration when used to treat acute respiratory failure. Reports of the effect of PEEP on cardiac output have been conflicting. We studied 14 patients and found that, although mean values of arterial oxygen tension (PaO2) increased as PEEP increased, on eight occasions in the acute studies and three in the elective studies, it fell as PEEP was increased. On five occasions the fall in PaO2 was associated with an increased shunt. Arterial pressure did not, on the average, change at any level of PEEP, but in 13 of 30 measurements it fell by more than 20% and necessitated curtailment of the study at blood pressure levels of less than 50 torr. Since these effects of PEEP cannot be predicted, careful patient monitoring is essential when ventilation with PEEP is utilized."}
{"id": "PMID:1096848", "title": "A chemosterilized antigen-extracted autodigested alloimplant for bone banks.", "content": "Limited chemical extraction of hydrophobic glycopeptides and subtotal autodigestion of the donor's cells and plasma membranes in undemineralized cortical bone in vitro reduces the putative quantity of haptenic substances absorbed by the recipient. Iodoacetic acid and sodium azide or other sulfhydryl group enzyme inhibitors added to the buffer solutions during in vitro autodigestion and estraction of intracellular alloantigens protects the bone matrix morphogenetic property against enzymatic degradation. The delayed hypersensitivity reaction induced by aseptically collected freeze-dried bone and the destruction of the bone morphogenetic property caused by radiation-sterilization is avoidable by sequential chemodigestion and chemosterilization of bone that preserves the maximum morphogenetic potential while transferring a minimum quantity of alloantigen.", "contents": "A chemosterilized antigen-extracted autodigested alloimplant for bone banks. Limited chemical extraction of hydrophobic glycopeptides and subtotal autodigestion of the donor's cells and plasma membranes in undemineralized cortical bone in vitro reduces the putative quantity of haptenic substances absorbed by the recipient. Iodoacetic acid and sodium azide or other sulfhydryl group enzyme inhibitors added to the buffer solutions during in vitro autodigestion and estraction of intracellular alloantigens protects the bone matrix morphogenetic property against enzymatic degradation. The delayed hypersensitivity reaction induced by aseptically collected freeze-dried bone and the destruction of the bone morphogenetic property caused by radiation-sterilization is avoidable by sequential chemodigestion and chemosterilization of bone that preserves the maximum morphogenetic potential while transferring a minimum quantity of alloantigen."}
{"id": "PMID:1096849", "title": "Blowhold cecostomy for cecal decompression.", "content": "Blowhole cecostomy is a method for achieving decompression of the distended cecum. Emphasis is placed on a McBurney-type incision, a watertight suture line joining bowel to muscle, and avoidance of opening the cecum until the suturing is done.", "contents": "Blowhold cecostomy for cecal decompression. Blowhole cecostomy is a method for achieving decompression of the distended cecum. Emphasis is placed on a McBurney-type incision, a watertight suture line joining bowel to muscle, and avoidance of opening the cecum until the suturing is done."}
{"id": "PMID:1096853", "title": "Mutagenic activity of cyclophosphamide, ifosfamide, and trofosfamide in different genes of escherichia coli and salmonella typhimurium after biotransformation through extracts of rodent liver.", "content": "Experiments are performed to compare the mutagenic properties of the three phosphamide esters of nitrogen mustard, cyclophosphamide (CP), ifosfamide (IF), and trofosfamide (TF), in different bacterial systems. The systems include forward mutations leading to resistance against 5-methyltryptophan (MTR) and from galR-s18 to gal-+ in Escherichia coli 343/113, back mutations from arg56 to arg-+ in Escherichia coli 343/113 and back mutations from hisG46 to his-+ in Salmonella typhimurium TA1535. CP, IF, and TF are not mutagenic per se. After biotransformation through isolated rodent liver homogenates (S-9 fraction) all three compounds exhibit mutagenic activity in the order CP smaller than IF smaller than TF. Specific activating potential of mouse liver extracts is higher than that of rat liver. Except for back mutations in S. typhimurium TA1535, all mutation systems tested show a similar pattern of induction after treatment with CP, IF, and TF. However, because gal-+ mutations are not induced by CP under conditions where arg-+ and MTR are induced, it is suggested that more than one mutational system be used in routine mutagenicity testing.", "contents": "Mutagenic activity of cyclophosphamide, ifosfamide, and trofosfamide in different genes of escherichia coli and salmonella typhimurium after biotransformation through extracts of rodent liver. Experiments are performed to compare the mutagenic properties of the three phosphamide esters of nitrogen mustard, cyclophosphamide (CP), ifosfamide (IF), and trofosfamide (TF), in different bacterial systems. The systems include forward mutations leading to resistance against 5-methyltryptophan (MTR) and from galR-s18 to gal-+ in Escherichia coli 343/113, back mutations from arg56 to arg-+ in Escherichia coli 343/113 and back mutations from hisG46 to his-+ in Salmonella typhimurium TA1535. CP, IF, and TF are not mutagenic per se. After biotransformation through isolated rodent liver homogenates (S-9 fraction) all three compounds exhibit mutagenic activity in the order CP smaller than IF smaller than TF. Specific activating potential of mouse liver extracts is higher than that of rat liver. Except for back mutations in S. typhimurium TA1535, all mutation systems tested show a similar pattern of induction after treatment with CP, IF, and TF. However, because gal-+ mutations are not induced by CP under conditions where arg-+ and MTR are induced, it is suggested that more than one mutational system be used in routine mutagenicity testing."}
{"id": "PMID:1096857", "title": "Interlamellar corneal grafts in rats. Effect of histocompatibility.", "content": "A technique of interlamellar keratoplasty was employed to evaluate the influence of difference in histocompatibility on the results of corneal transplantation in highly inbred rats. The degree of corneal clarity and vascularity and the incidence of rejection of relatively conpatible (\"weak\") and incompatible (\"strong\") allografts were recorded blindly in recipients with normal and vascularized corneas and in recipients sensitized with donor tissue either before or after corneal grafting. In contrast to the well-known effects of histocompatibility on skin and organ allografts, there was no substantial effect whatsoever of histocompatibility on either the severity of corneal allograft reactions orthe incidence of rejection. Consequently, the results of this experimental investigation suggest that tissue typing may have little or no value in clinical keratoplasty.", "contents": "Interlamellar corneal grafts in rats. Effect of histocompatibility. A technique of interlamellar keratoplasty was employed to evaluate the influence of difference in histocompatibility on the results of corneal transplantation in highly inbred rats. The degree of corneal clarity and vascularity and the incidence of rejection of relatively conpatible (\"weak\") and incompatible (\"strong\") allografts were recorded blindly in recipients with normal and vascularized corneas and in recipients sensitized with donor tissue either before or after corneal grafting. In contrast to the well-known effects of histocompatibility on skin and organ allografts, there was no substantial effect whatsoever of histocompatibility on either the severity of corneal allograft reactions orthe incidence of rejection. Consequently, the results of this experimental investigation suggest that tissue typing may have little or no value in clinical keratoplasty."}
{"id": "PMID:1096858", "title": "Corneal epithelial preservation.", "content": "While clinical experience has demonstrated that current storage techniques are adequate for the preservation of human endothelial cells, increased use of donor epithelium in cases of severe disturbance to epithelial and stromal integrity requires a reexamination of storage effects on the epithelial layer. Measurement of human and rabbit metabolite levels in stored corneas indicates that the epithelium is maintained in a normal state only if the eye is cooled promptly. If cold storage is delayed for as little as one hour after death, glucose levels in the stroma and aqueous fall below levels known to be required for preservation of epithelial integrity. The fundamental time of interest in preservation of donor corneal tissue is in the interval between death and cooling of the eye. If prompt enucleation is not possible, ice should be applied to maintain viability of the epithelium.", "contents": "Corneal epithelial preservation. While clinical experience has demonstrated that current storage techniques are adequate for the preservation of human endothelial cells, increased use of donor epithelium in cases of severe disturbance to epithelial and stromal integrity requires a reexamination of storage effects on the epithelial layer. Measurement of human and rabbit metabolite levels in stored corneas indicates that the epithelium is maintained in a normal state only if the eye is cooled promptly. If cold storage is delayed for as little as one hour after death, glucose levels in the stroma and aqueous fall below levels known to be required for preservation of epithelial integrity. The fundamental time of interest in preservation of donor corneal tissue is in the interval between death and cooling of the eye. If prompt enucleation is not possible, ice should be applied to maintain viability of the epithelium."}
{"id": "PMID:1096860", "title": "[Studies on soft tissue reactions to implantation of glass ceramics (author's transl)].", "content": "This report describes the implantation of glass and glass-ceramics into soft tissues. These experiments revealed that our new glass and glass-ceramic materials are well compatible with soft tissues. The fibrous membrane surrounding such an implantat consists of normal dense collageneous connective tissue. The cells are fibroblasts and fibrocyt-like cells without any abnormal morphology. Macrophages could not be detected. However, the degree of implants solubility must be chosen with care, because high solubility of the implant increases the reaction of the surrounding soft tissues. The described materials are also compatible with osseous tissues. In our previous experiments we have already pointed out, that bonding developed between glass material and bone. Few weeks after implatation we have seen mature lamellar homegeneously mineralized bone opposed to the implant. There was no fibrous membrane between implant and bone. Longtime studies show that hitherto the described bonding exists about 2 years.", "contents": "[Studies on soft tissue reactions to implantation of glass ceramics (author's transl)]. This report describes the implantation of glass and glass-ceramics into soft tissues. These experiments revealed that our new glass and glass-ceramic materials are well compatible with soft tissues. The fibrous membrane surrounding such an implantat consists of normal dense collageneous connective tissue. The cells are fibroblasts and fibrocyt-like cells without any abnormal morphology. Macrophages could not be detected. However, the degree of implants solubility must be chosen with care, because high solubility of the implant increases the reaction of the surrounding soft tissues. The described materials are also compatible with osseous tissues. In our previous experiments we have already pointed out, that bonding developed between glass material and bone. Few weeks after implatation we have seen mature lamellar homegeneously mineralized bone opposed to the implant. There was no fibrous membrane between implant and bone. Longtime studies show that hitherto the described bonding exists about 2 years."}
{"id": "PMID:1096861", "title": "Acupuncture for sensorineural hearing loss.", "content": "Forty children, aged 9 to 16 years, with severe and profound sensorineural hearing loss received acupuncture five times weekly for three out of four weeks for a period of six months.  The children had weekly audiometric evaluations.  There were no clinically important differences during and post acupuncture.", "contents": "Acupuncture for sensorineural hearing loss. Forty children, aged 9 to 16 years, with severe and profound sensorineural hearing loss received acupuncture five times weekly for three out of four weeks for a period of six months.  The children had weekly audiometric evaluations.  There were no clinically important differences during and post acupuncture."}
{"id": "PMID:1096862", "title": "Effect of the high potassium medium on cultured cochlear epithelial cells.", "content": "1. A high potassium and low sodium culture medium does not produce morphological change in cochlear epithelial cells such as sulcus cell, Deiters' cell, Hensen's cell, Claudius' cell and Raissner's membrane cell. 2. Interdental cells of spiral limbus are affected by a high potassium medium but the change is to a moderate degree. 3. Outer hair cells degenerate quickly in a high potassium and low sodium medium. The onset and the grade of the degeneration is in a parallel with the potassium concentration, and a concentration of more than 46.4 mEq/l produces an acute and severe degeneration of outer hair cells.", "contents": "Effect of the high potassium medium on cultured cochlear epithelial cells. 1. A high potassium and low sodium culture medium does not produce morphological change in cochlear epithelial cells such as sulcus cell, Deiters' cell, Hensen's cell, Claudius' cell and Raissner's membrane cell. 2. Interdental cells of spiral limbus are affected by a high potassium medium but the change is to a moderate degree. 3. Outer hair cells degenerate quickly in a high potassium and low sodium medium. The onset and the grade of the degeneration is in a parallel with the potassium concentration, and a concentration of more than 46.4 mEq/l produces an acute and severe degeneration of outer hair cells."}
{"id": "PMID:1096866", "title": "Temporal and masking phenomena in persons with sensorineural hearing loss.", "content": "Relatively few experimental investigations have studied backward or forward masking, masking level differences, or differential frequency effects in simultaneous masking among persons with hearing impairment. This is regrettable since such efforts may lead to important new understandings of auditory processes. This paper reviews available data, discusses some precautions of experimental procedure, and offers suggestions for future research directions.", "contents": "Temporal and masking phenomena in persons with sensorineural hearing loss. Relatively few experimental investigations have studied backward or forward masking, masking level differences, or differential frequency effects in simultaneous masking among persons with hearing impairment. This is regrettable since such efforts may lead to important new understandings of auditory processes. This paper reviews available data, discusses some precautions of experimental procedure, and offers suggestions for future research directions."}
{"id": "PMID:1096867", "title": "Masking level difference: a measure of auditory processing capability.", "content": "MLDs are evidence of the superiority of the binaural auditory system. Cochlear lesions do not necessarily impair the MLD and persons with cortical lesions are also able to produce normal MLDs. The evidence for intermediate level auditory pathway lesions is more equivocal. The MLD is not the only measure of binaural hearing and binaural hearing is itself only one measure of a number of complex auditory processes. In this experiment measures of MLDs, localization, temporal integration, central masking and difference limen for intensity were made on 11 persons with normal hearing. Correlational analyses including factor analysis indicated that the MLD is a fairly independent measure but that central masking, brief tone audiometry and difference limen for intensity represent related auditory tasks. The relationship of localization tasks in this picture is unclear.", "contents": "Masking level difference: a measure of auditory processing capability. MLDs are evidence of the superiority of the binaural auditory system. Cochlear lesions do not necessarily impair the MLD and persons with cortical lesions are also able to produce normal MLDs. The evidence for intermediate level auditory pathway lesions is more equivocal. The MLD is not the only measure of binaural hearing and binaural hearing is itself only one measure of a number of complex auditory processes. In this experiment measures of MLDs, localization, temporal integration, central masking and difference limen for intensity were made on 11 persons with normal hearing. Correlational analyses including factor analysis indicated that the MLD is a fairly independent measure but that central masking, brief tone audiometry and difference limen for intensity represent related auditory tasks. The relationship of localization tasks in this picture is unclear."}
{"id": "PMID:1096868", "title": "Auditory localization and its clinical applications.", "content": "Factors of significance in the localization of sound are considered. The ability of hearing-impaired subjects to localize noise in the horizontal plane was examined with and without hearing aids, and also compared with the results for normally hearing subjects. The directional hearing was not found to be improved by the hearing aids used. The ability to localize sound has been used by several investigators as a diagnostic tool: poor directional hearing may be expected in patients with lesions of the cochlear nerve or the pontine region. However, other factors are also of significance and may impair the localization of sound. These problems are discussed.", "contents": "Auditory localization and its clinical applications. Factors of significance in the localization of sound are considered. The ability of hearing-impaired subjects to localize noise in the horizontal plane was examined with and without hearing aids, and also compared with the results for normally hearing subjects. The directional hearing was not found to be improved by the hearing aids used. The ability to localize sound has been used by several investigators as a diagnostic tool: poor directional hearing may be expected in patients with lesions of the cochlear nerve or the pontine region. However, other factors are also of significance and may impair the localization of sound. These problems are discussed."}
{"id": "PMID:1096865", "title": "Some effects of prestimulus activity and length of prestimulus observation on judgments of newborns' responses to sounds.", "content": "Previous work on the effects of prestimulus state on newborn auditory response is briefly reviewed. Subjectively assessed responses of nine newborns by five observers were studied as a function of (1) prestimulus activity level and (2) duration of prestimulus observation period. Response assessment was significantly affected for the former but not by the latter. For sounds which elicited a high proportion of responses, the prestimulus activity level had relatively little effect on judgment of response, whereas for sounds (and no-sound \"control\" trials) to which few responses were ascribed, prestimulus activity tended to be associated with positive ratings of response.", "contents": "Some effects of prestimulus activity and length of prestimulus observation on judgments of newborns' responses to sounds. Previous work on the effects of prestimulus state on newborn auditory response is briefly reviewed. Subjectively assessed responses of nine newborns by five observers were studied as a function of (1) prestimulus activity level and (2) duration of prestimulus observation period. Response assessment was significantly affected for the former but not by the latter. For sounds which elicited a high proportion of responses, the prestimulus activity level had relatively little effect on judgment of response, whereas for sounds (and no-sound \"control\" trials) to which few responses were ascribed, prestimulus activity tended to be associated with positive ratings of response."}
{"id": "PMID:1096871", "title": "Instrument for the on-line measurement of the slow phase of nystagmus.", "content": "The on-line measurement of the slow-phase velocity of vestibular nystagmus, induced or spontaneous, has considerable practical advantages. An instrument for this purpose, developed around simple operational amplifiers, is described. Typical applications of the system are given in respect of nystagmus induced by optokinetic, rotational, and caloric stimuli.", "contents": "Instrument for the on-line measurement of the slow phase of nystagmus. The on-line measurement of the slow-phase velocity of vestibular nystagmus, induced or spontaneous, has considerable practical advantages. An instrument for this purpose, developed around simple operational amplifiers, is described. Typical applications of the system are given in respect of nystagmus induced by optokinetic, rotational, and caloric stimuli."}
{"id": "PMID:1096872", "title": "Relationship between virus multiplication and culture cells synchronized by excess thymidine treatment. V. Effect of chikungunya virus infection upon the host cell cycle.", "content": "The cell cycle of FL cells infected with chikungunya virus was examined using cultures synchronized with excess thymidine. Viral growth at the cellular level was demonstrated by the presence of viral antigen and viral RNA synthesis. It was found that cells which had been infected with virus before the S phase, could enter the S phase. Furthermore,, cells infected in the phase before the M phase could enter the M phase. However, infected cells showed abrupt karyorrhexis after a certain period of infection. This contrasts with studies on pox virus-infected cells, where the cell cycle dose not proceed once viral-DNA synthesis is initiated at any phase of the cell cycle.", "contents": "Relationship between virus multiplication and culture cells synchronized by excess thymidine treatment. V. Effect of chikungunya virus infection upon the host cell cycle. The cell cycle of FL cells infected with chikungunya virus was examined using cultures synchronized with excess thymidine. Viral growth at the cellular level was demonstrated by the presence of viral antigen and viral RNA synthesis. It was found that cells which had been infected with virus before the S phase, could enter the S phase. Furthermore,, cells infected in the phase before the M phase could enter the M phase. However, infected cells showed abrupt karyorrhexis after a certain period of infection. This contrasts with studies on pox virus-infected cells, where the cell cycle dose not proceed once viral-DNA synthesis is initiated at any phase of the cell cycle."}
{"id": "PMID:1096873", "title": "The effect of spermine on transcription of mammalian chromatin by mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerase.", "content": "Isolated rat liver nuclei demonstrate an increased ability to synthesize RNA in the presence of either spermine or spermidine. Spermidine has more effect on the low-salt alpha-amanitin-insensitive reaction, and spermine has more effect on the high-salt alpha-amanitin-sensitive reaction. Spermine is effective at concentrations of 0.1 mM and 1 muM, showing a biphasic effect. The RNA polymerase activity associated with nuclear chromatin is increased in the presence of spermine only at a concentration of 0.1 mM. Aso the transcription of deproteinized liver DNA by liver form-B polymerase or Escherichia coli enzyme is more efficient in the presence of 0.1 mM-spermine. Only when liver chromatin is transcribed by its homologous enzyme (and not by E. coli enzyme) is spermine active at both 0.1mM and 1 muM as in purified nuclei. The lower concentration of spermine (1 muM) is able to affect chromatin transcription by increasing the affinity of chromatin for the enzyme. Our findings suggest a regulatory role of spermine at the level of genome transcription.", "contents": "The effect of spermine on transcription of mammalian chromatin by mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerase. Isolated rat liver nuclei demonstrate an increased ability to synthesize RNA in the presence of either spermine or spermidine. Spermidine has more effect on the low-salt alpha-amanitin-insensitive reaction, and spermine has more effect on the high-salt alpha-amanitin-sensitive reaction. Spermine is effective at concentrations of 0.1 mM and 1 muM, showing a biphasic effect. The RNA polymerase activity associated with nuclear chromatin is increased in the presence of spermine only at a concentration of 0.1 mM. Aso the transcription of deproteinized liver DNA by liver form-B polymerase or Escherichia coli enzyme is more efficient in the presence of 0.1 mM-spermine. Only when liver chromatin is transcribed by its homologous enzyme (and not by E. coli enzyme) is spermine active at both 0.1mM and 1 muM as in purified nuclei. The lower concentration of spermine (1 muM) is able to affect chromatin transcription by increasing the affinity of chromatin for the enzyme. Our findings suggest a regulatory role of spermine at the level of genome transcription."}
{"id": "PMID:1096900", "title": "[Clinical-pharmacological and pharmacokinetic studies with naproxen].", "content": "d-2-(6'-Methoxy-2'-naphthyl)-propionic acid (naproxen) is bound in blood to a high degree to plasma protein. After oral application naproxen is absorbed rapidly and completely. The mean biological half-life in man is fourteen hours. This gives us the possibility of controlling the symptoms of disease with two daily doses. In man, 99% of naproxen is excreted in the urine, either unchanged or glucuronized and as its 6-des-methyl metabolite. There is a linear increase of plasma levels with daily doses of up to 500 mg. Higher naproxen doses do not produce higher blood levels but lead to a faster excretion. This probably depends in part on a saturation of the binding sites in plasma. Differences in affinity of some compounds for binding sites in plasma are responsible for a potential interaction between naproxen and other agents, such as warfarin, acetyl salicylic acid (ASA) and sulfonylureas. These interactions are discussed. In a double-blind trial the influence of naproxen and ASA on the gastric mucosa of healthy volunteers has been compared. The results of gastroscopic findings as well as laboratory tests are reported.", "contents": "[Clinical-pharmacological and pharmacokinetic studies with naproxen]. d-2-(6'-Methoxy-2'-naphthyl)-propionic acid (naproxen) is bound in blood to a high degree to plasma protein. After oral application naproxen is absorbed rapidly and completely. The mean biological half-life in man is fourteen hours. This gives us the possibility of controlling the symptoms of disease with two daily doses. In man, 99% of naproxen is excreted in the urine, either unchanged or glucuronized and as its 6-des-methyl metabolite. There is a linear increase of plasma levels with daily doses of up to 500 mg. Higher naproxen doses do not produce higher blood levels but lead to a faster excretion. This probably depends in part on a saturation of the binding sites in plasma. Differences in affinity of some compounds for binding sites in plasma are responsible for a potential interaction between naproxen and other agents, such as warfarin, acetyl salicylic acid (ASA) and sulfonylureas. These interactions are discussed. In a double-blind trial the influence of naproxen and ASA on the gastric mucosa of healthy volunteers has been compared. The results of gastroscopic findings as well as laboratory tests are reported."}
{"id": "PMID:1096901", "title": "A Double-blind comparison of naproxen against indometacin in osteoarthrosis.", "content": "Fifty patients with osteoarthrosis of the knee and hip were entered into a double-blind trial of d-2-(6'-methoxy-2-naphthyl)-propionic acid (naproxen) 500 mg daily versus indometacin 100 mg daily. Two simultaneous trials of identical design were performed, one consisting of patients with unilateral or bilateral knee joint arthrosis only, the other of patients with unilateral or bilateral hip joint disease. Patients were assigned to 4 weeks treatment on naproxen or  indometacin, then crossed over to the other drug. Assessments were made at --2, 0, 2, 4, 6 and 8 weeks, including subjective grading of pain and objective measurement of joint movements, stair climbing time and walking time. In almost all parameters there was significant improvement from baseline on both drugs, the magnitude of improvement being statistically equivalent. Side-effects recorded during the naproxen treatment period were significantly fewer than during indometacin treatment.", "contents": "A Double-blind comparison of naproxen against indometacin in osteoarthrosis. Fifty patients with osteoarthrosis of the knee and hip were entered into a double-blind trial of d-2-(6'-methoxy-2-naphthyl)-propionic acid (naproxen) 500 mg daily versus indometacin 100 mg daily. Two simultaneous trials of identical design were performed, one consisting of patients with unilateral or bilateral knee joint arthrosis only, the other of patients with unilateral or bilateral hip joint disease. Patients were assigned to 4 weeks treatment on naproxen or  indometacin, then crossed over to the other drug. Assessments were made at --2, 0, 2, 4, 6 and 8 weeks, including subjective grading of pain and objective measurement of joint movements, stair climbing time and walking time. In almost all parameters there was significant improvement from baseline on both drugs, the magnitude of improvement being statistically equivalent. Side-effects recorded during the naproxen treatment period were significantly fewer than during indometacin treatment."}
{"id": "PMID:1096902", "title": "[Methods and statistics of multicentral double-blind \"cross-over\" examination of naproxen compared to indomethacin].", "content": "The methodology of documenting clinical findings and statistical analysis of the results of a multicentre double-blind cross-over study, in which the efficacy of d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) and indometacin in the treatment of rheumatic diseases was compared, is presented. Eight investigators from four clinics conducted this clinical trial according to an identical protocol in a total of 100 patients. 46 patients (5 male and 31 female) with rheumatoid arthritis received either of the two drugs over a period of 26 days. 35 patients (32 male and 3 female) with ankylosing spondylitis and 19 patients (8 male and 11 female) with osteoarthrosis were treated with either of the drugs for 15 days. The following parameters were investigated and documented: pain and its localization, inhibition of joint function, symptoms of inflammation, and the \"quasi\" irreversible joint changes. These various parameters were for statistical purposes combined as indices for pain, function, and inflammation. This permitted a quantitative evaluation of the analgesic and antiinflammatory efficacy as well as of the improvement of joint function of the two compounds tested. The overall results of the clinical parameters revealed no significant difference in efficacy for the two drugs. When differentiating the patients as to their sex, both drugs showed higher efficacy in male patients, and only slight efficacy in female patients.", "contents": "[Methods and statistics of multicentral double-blind \"cross-over\" examination of naproxen compared to indomethacin]. The methodology of documenting clinical findings and statistical analysis of the results of a multicentre double-blind cross-over study, in which the efficacy of d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) and indometacin in the treatment of rheumatic diseases was compared, is presented. Eight investigators from four clinics conducted this clinical trial according to an identical protocol in a total of 100 patients. 46 patients (5 male and 31 female) with rheumatoid arthritis received either of the two drugs over a period of 26 days. 35 patients (32 male and 3 female) with ankylosing spondylitis and 19 patients (8 male and 11 female) with osteoarthrosis were treated with either of the drugs for 15 days. The following parameters were investigated and documented: pain and its localization, inhibition of joint function, symptoms of inflammation, and the \"quasi\" irreversible joint changes. These various parameters were for statistical purposes combined as indices for pain, function, and inflammation. This permitted a quantitative evaluation of the analgesic and antiinflammatory efficacy as well as of the improvement of joint function of the two compounds tested. The overall results of the clinical parameters revealed no significant difference in efficacy for the two drugs. When differentiating the patients as to their sex, both drugs showed higher efficacy in male patients, and only slight efficacy in female patients."}
{"id": "PMID:1096903", "title": "[Clinical results of a multicentral double-blind examination of naproxen compared to indomethacin in chronic rheumatoid arthritis, ankylosing spondylitis, and osteoarthrosis].", "content": "In a multicenter double-blind cross-over trial the therapeutic effect and the tolerance of d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) and indometacin were compared including 46 patients with rheumatoid arthritis, 35 patients with ankylosing spondylitis and 19 patients with osteoarthrosis. Duration of treatment with both drugs was two to four weeks each. The daily dose of naproxen was 750 mg, that of indometacin l50 mg. In rheumatoid arthritis the combined clinical effect of indometacin was stronger than that of naproxen while both drugs had the same clinical effectiveness in ankylosing spondylitis and osteoarthrosis. When differentiating the total clinical effect as to indices of pain, inflammation and function, indometacin was shown to be superior in all three diseases with regard to pain index. On the other hand, naproxen showed a better effect in ankylosing spondylitis and osteoarthrosis than indometacin as to inflammation, and as to function in osteoarthrosis.", "contents": "[Clinical results of a multicentral double-blind examination of naproxen compared to indomethacin in chronic rheumatoid arthritis, ankylosing spondylitis, and osteoarthrosis]. In a multicenter double-blind cross-over trial the therapeutic effect and the tolerance of d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) and indometacin were compared including 46 patients with rheumatoid arthritis, 35 patients with ankylosing spondylitis and 19 patients with osteoarthrosis. Duration of treatment with both drugs was two to four weeks each. The daily dose of naproxen was 750 mg, that of indometacin l50 mg. In rheumatoid arthritis the combined clinical effect of indometacin was stronger than that of naproxen while both drugs had the same clinical effectiveness in ankylosing spondylitis and osteoarthrosis. When differentiating the total clinical effect as to indices of pain, inflammation and function, indometacin was shown to be superior in all three diseases with regard to pain index. On the other hand, naproxen showed a better effect in ankylosing spondylitis and osteoarthrosis than indometacin as to inflammation, and as to function in osteoarthrosis."}
{"id": "PMID:1096904", "title": "[A double blind comparison of naproxen and indomethacin on the after-midnight-pain of patients with morbus bechterew].", "content": "27 patients with ascertained ankylosing spondylitis experiencing constant after-midnight-pain received, following three medication-free days, in a double blind, randomized, cross-over fashion indometacin (100 g/day) and d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) (500 mg/day), as suppositories, for a period of six days each. The intensity of the night-pain was recorded daily. Naproxen was shown to be equally effective as indometacin in alleviating the after-midnight backache of ankylosing spondylitis. Side effects occurred under indometacin in 5 cases, under naproxen in 3 cases.", "contents": "[A double blind comparison of naproxen and indomethacin on the after-midnight-pain of patients with morbus bechterew]. 27 patients with ascertained ankylosing spondylitis experiencing constant after-midnight-pain received, following three medication-free days, in a double blind, randomized, cross-over fashion indometacin (100 g/day) and d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) (500 mg/day), as suppositories, for a period of six days each. The intensity of the night-pain was recorded daily. Naproxen was shown to be equally effective as indometacin in alleviating the after-midnight backache of ankylosing spondylitis. Side effects occurred under indometacin in 5 cases, under naproxen in 3 cases."}
{"id": "PMID:1096905", "title": "[Observations of long-term treatment of arthrosis with naproxen].", "content": "In a previous open clinical trial with d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) in 32 outpatients with osteoarthrosis, very good to good results were achieved in 6 patients, satisfactory in 12, fair in 5, and unsatisfactory in 9 patients. The severest side effect observed was a case of gastric bleeding in a female patient who had been previously treated with a drug containing phenylbutazone and prednisone. Since, furthermore, 6 out of 9 such patients who did not respond to therapy had to stop the durg prematurely because of minor side effects and since in another 6 there was a tendency to a reduction of the leucocyte count, we subsequently initiated a long-term efficacy and tolerance study in our department for chronic patients. This series comprised 15 patients, aged 66 to 86 years, with severe, partly immobilizing coxarthroses and gonarthroses. The length of treatment was 2 to 16 months (mean 10.5 months). During the first 3 months ESR determinations and complete blood counts were performed every two weeks, later at four-weekly intervals. The patients were questioned weekly as to the efficacy of the treatment and as to side effects. Neither changes of the laboratory data nor gastro-intestinal side effects were observed. In two patients -- one a female with multiple allergies -- treatment had to be stopped because of itching and exanthema, respectively. The analgesic effect of naproxen was evaluated as very good in 5 cases, good in 5, fair in 2, and unsatisfactory in 3 cases. In addition, we conducted another clinical trial in 15 outpatients with degenerative joint diseases (partly of the spinal column). Their age varied from 16 to 60 years. Naproxen was administered in daily doses from 500 to 750 mg for the duration of 6 to 12 weeks. In no case had treatment to be interrupted, nor was there any gastric intolerance or alteration of the blood count observed in this group. Clinical results were classified as satisfactory to good in 10 patients.", "contents": "[Observations of long-term treatment of arthrosis with naproxen]. In a previous open clinical trial with d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen) in 32 outpatients with osteoarthrosis, very good to good results were achieved in 6 patients, satisfactory in 12, fair in 5, and unsatisfactory in 9 patients. The severest side effect observed was a case of gastric bleeding in a female patient who had been previously treated with a drug containing phenylbutazone and prednisone. Since, furthermore, 6 out of 9 such patients who did not respond to therapy had to stop the durg prematurely because of minor side effects and since in another 6 there was a tendency to a reduction of the leucocyte count, we subsequently initiated a long-term efficacy and tolerance study in our department for chronic patients. This series comprised 15 patients, aged 66 to 86 years, with severe, partly immobilizing coxarthroses and gonarthroses. The length of treatment was 2 to 16 months (mean 10.5 months). During the first 3 months ESR determinations and complete blood counts were performed every two weeks, later at four-weekly intervals. The patients were questioned weekly as to the efficacy of the treatment and as to side effects. Neither changes of the laboratory data nor gastro-intestinal side effects were observed. In two patients -- one a female with multiple allergies -- treatment had to be stopped because of itching and exanthema, respectively. The analgesic effect of naproxen was evaluated as very good in 5 cases, good in 5, fair in 2, and unsatisfactory in 3 cases. In addition, we conducted another clinical trial in 15 outpatients with degenerative joint diseases (partly of the spinal column). Their age varied from 16 to 60 years. Naproxen was administered in daily doses from 500 to 750 mg for the duration of 6 to 12 weeks. In no case had treatment to be interrupted, nor was there any gastric intolerance or alteration of the blood count observed in this group. Clinical results were classified as satisfactory to good in 10 patients."}
{"id": "PMID:1096907", "title": "Sinus node function in the denervated human heart. Effect of digitalis.", "content": "Evaluation of sinus node function was performed in 5 patients with an intact cardiac autonomic nervous system (group I), and in 8 patients with a transplantated, denervated heart (group 2). After baseline data were recorded, the electrophysiological studies were repeated in all group I patients and in 6 of the 8 group 2 patients, 45 to 60 minutes after the administration of digoxin 1.25 mg intravenously. Baseline cycle length, sinus node recovery time, and sinoatrial conduction time were significantly shorter in the transplanted heart than in those with intact autonomic innervation, but correction of the sinus node recovery time and sinoatrial conduction time for heart rate abolished these differences. Digoxin produced a small increase in cycle length, sinus node recovery time, and sinoatrial conduction time which did not reach statistical significance in this small study group of patients with innervated hearts. In the denervated, transplanted patients, no change in cycle length occurred after digoxin in any patient. The sinus node recovery time was unaffected by glycoside administration in 3 of 6 patients, while the sinoatrial conduction time was unchanged in 4 of 6. In one group 2 patient, digoxin produced first degree sinoatrial node exit block, and in a second patient, 2:1 sinoatrial nodal exit block developed. The mechanisms responsible for these effects in the denervated heart are not clear.", "contents": "Sinus node function in the denervated human heart. Effect of digitalis. Evaluation of sinus node function was performed in 5 patients with an intact cardiac autonomic nervous system (group I), and in 8 patients with a transplantated, denervated heart (group 2). After baseline data were recorded, the electrophysiological studies were repeated in all group I patients and in 6 of the 8 group 2 patients, 45 to 60 minutes after the administration of digoxin 1.25 mg intravenously. Baseline cycle length, sinus node recovery time, and sinoatrial conduction time were significantly shorter in the transplanted heart than in those with intact autonomic innervation, but correction of the sinus node recovery time and sinoatrial conduction time for heart rate abolished these differences. Digoxin produced a small increase in cycle length, sinus node recovery time, and sinoatrial conduction time which did not reach statistical significance in this small study group of patients with innervated hearts. In the denervated, transplanted patients, no change in cycle length occurred after digoxin in any patient. The sinus node recovery time was unaffected by glycoside administration in 3 of 6 patients, while the sinoatrial conduction time was unchanged in 4 of 6. In one group 2 patient, digoxin produced first degree sinoatrial node exit block, and in a second patient, 2:1 sinoatrial nodal exit block developed. The mechanisms responsible for these effects in the denervated heart are not clear."}
{"id": "PMID:1096908", "title": "Evaluation of the toxicity of local anaesthetic agents in man.", "content": "Etidocaine given by intravenous infusion has been compared, using a double-blind technique, with bupivacaine and lignocaine in respect of toxic symptoms and signs. The degree of toxicity is affected considerably by the rate of drug infusion. At 10 mg/min subjects could tolerate twice the dose of etidocaine as bupivacaine. Tolerance to etidocaine was less at 20 mg/min but still compared favourably with bupivacaine at 10 mg/min. Considering the difference in potency of the two agents it was considered that the therapeutic ratios would not be substantially different. Lignocaine at 20 mg/min was better tolerated than etidocaine. Venous plasma concentration during these experiments showed a more rapid decrease in the case of etidocaine compared with bupivacaine, but the concentrations were unhelpful in predicting the toxic effects. Similarly electroencephalography revealed no abnormalities in spite of marked subjective and objective signs of toxicity.", "contents": "Evaluation of the toxicity of local anaesthetic agents in man. Etidocaine given by intravenous infusion has been compared, using a double-blind technique, with bupivacaine and lignocaine in respect of toxic symptoms and signs. The degree of toxicity is affected considerably by the rate of drug infusion. At 10 mg/min subjects could tolerate twice the dose of etidocaine as bupivacaine. Tolerance to etidocaine was less at 20 mg/min but still compared favourably with bupivacaine at 10 mg/min. Considering the difference in potency of the two agents it was considered that the therapeutic ratios would not be substantially different. Lignocaine at 20 mg/min was better tolerated than etidocaine. Venous plasma concentration during these experiments showed a more rapid decrease in the case of etidocaine compared with bupivacaine, but the concentrations were unhelpful in predicting the toxic effects. Similarly electroencephalography revealed no abnormalities in spite of marked subjective and objective signs of toxicity."}
{"id": "PMID:1096909", "title": "Change in end-tidal position in children after suxamethonium.", "content": "The change in end-tidal position (ETP) after suxamethonium-induced paralysis was measured in 15 children during routine general anaesthesia. In all patients the onset of muscle paralysis was associated with an increase in lung volume from ETP. This increase may be the result of paralysis of the expiratory muscles which are active during anaesthesia.", "contents": "Change in end-tidal position in children after suxamethonium. The change in end-tidal position (ETP) after suxamethonium-induced paralysis was measured in 15 children during routine general anaesthesia. In all patients the onset of muscle paralysis was associated with an increase in lung volume from ETP. This increase may be the result of paralysis of the expiratory muscles which are active during anaesthesia."}
{"id": "PMID:1096915", "title": "Effect of CO2 on myocardial contractility and aortic input impedance during anaesthesia.", "content": "The haemodynamic responses to hypocapnia and hypercapnia have been studied in the dog during intermittent positive pressure ventilation under halothane anaesthesia (1% halothane in oxygen) and under nitrous oxide anaesthesia (30% oxygen in nitrous oxide). In the absence of significant variations of either myocardial contractility or left ventricular end-diastolic pressure, the changes of stroke volume and cardiac output (diminution because of hypocapnia, augmentation because of hypercapnia) were determined by alterations of systemic vascular resistance (augmentation because of hypocapnia, diminution because of hypercapnia).", "contents": "Effect of CO2 on myocardial contractility and aortic input impedance during anaesthesia. The haemodynamic responses to hypocapnia and hypercapnia have been studied in the dog during intermittent positive pressure ventilation under halothane anaesthesia (1% halothane in oxygen) and under nitrous oxide anaesthesia (30% oxygen in nitrous oxide). In the absence of significant variations of either myocardial contractility or left ventricular end-diastolic pressure, the changes of stroke volume and cardiac output (diminution because of hypocapnia, augmentation because of hypercapnia) were determined by alterations of systemic vascular resistance (augmentation because of hypocapnia, diminution because of hypercapnia)."}
{"id": "PMID:1096916", "title": "Mechanics of breathing, gas distribution and functional residual capacity at different frequencies of respiration during spontaneous and artificial ventilation.", "content": "Nine healthy volunteers were investigated, both while awake and breathing spontaneously, and while anaesthetized with IPPV, in all cases at rates of both 12 and 24 b.p.m. Gas flow and volume were measured with a pneumotachography. The transpulmonary pressure (the pressure difference between the trachea or the buccal cavity and the oesophagus) was also recorded. The distribution of gas was analysed by means of nitrogen washout curves, which also permitted the determination of functional residual capacity (FRC). Lung compliance during IPPV was approximately half that during spontaneous breathing. During IPPV the compliance was dependent on the frequency of ventilation, being lower with the greater frequency. Pulmonary resistance was approximately twice as great with artificial ventilation, but no significant relationship to frequency was demonstrated. Gas distribution was within normal limits and in this respect there was no difference between low and high rates of spontaneous breathing. With IPPV at the higher rate, gas distribution was significantly less even, but still within normal limits. Dfferences in FRC under the different conditions during the experiments were not significant, but the values obtained were lower with artificial ventilation. Neither the reduction in dynamic lung compliance induced by anaesthesia and artificial ventilation, nor its dependence on the frequency of such ventilation, can be explained with certainty by changes in gas distribution.", "contents": "Mechanics of breathing, gas distribution and functional residual capacity at different frequencies of respiration during spontaneous and artificial ventilation. Nine healthy volunteers were investigated, both while awake and breathing spontaneously, and while anaesthetized with IPPV, in all cases at rates of both 12 and 24 b.p.m. Gas flow and volume were measured with a pneumotachography. The transpulmonary pressure (the pressure difference between the trachea or the buccal cavity and the oesophagus) was also recorded. The distribution of gas was analysed by means of nitrogen washout curves, which also permitted the determination of functional residual capacity (FRC). Lung compliance during IPPV was approximately half that during spontaneous breathing. During IPPV the compliance was dependent on the frequency of ventilation, being lower with the greater frequency. Pulmonary resistance was approximately twice as great with artificial ventilation, but no significant relationship to frequency was demonstrated. Gas distribution was within normal limits and in this respect there was no difference between low and high rates of spontaneous breathing. With IPPV at the higher rate, gas distribution was significantly less even, but still within normal limits. Dfferences in FRC under the different conditions during the experiments were not significant, but the values obtained were lower with artificial ventilation. Neither the reduction in dynamic lung compliance induced by anaesthesia and artificial ventilation, nor its dependence on the frequency of such ventilation, can be explained with certainty by changes in gas distribution."}
{"id": "PMID:1096917", "title": "An autoclavable ventilation failure alarm.", "content": "A small ventilation failure alarm which can be used with all types of lung ventilator is described. The entire patient circuit can be removed and autoclaved easily. Activation of the unit is by a removable key, a mains failure warning is provided, and alarm may be muted during aspiration of secretions from the tracheobronchial tree.", "contents": "An autoclavable ventilation failure alarm. A small ventilation failure alarm which can be used with all types of lung ventilator is described. The entire patient circuit can be removed and autoclaved easily. Activation of the unit is by a removable key, a mains failure warning is provided, and alarm may be muted during aspiration of secretions from the tracheobronchial tree."}
{"id": "PMID:1096918", "title": "Mechanism of action of extradural analgesia.", "content": "The factors governing the spread and site of action of local anaesthetics in the extradural space are discussed. Local anaesthetic drugs injected into the extradural space may cause nerve blocks at three possible sites: (a) On the spinal nerves in the paravertebral space. This probably occurs only in young subjects. (b) On the spinal nerves intradurally. This is probably the essential site of blockade. (c) On the spinal cord.", "contents": "Mechanism of action of extradural analgesia. The factors governing the spread and site of action of local anaesthetics in the extradural space are discussed. Local anaesthetic drugs injected into the extradural space may cause nerve blocks at three possible sites: (a) On the spinal nerves in the paravertebral space. This probably occurs only in young subjects. (b) On the spinal nerves intradurally. This is probably the essential site of blockade. (c) On the spinal cord."}
{"id": "PMID:1096919", "title": "A review of the metabolism of amide local anaesthetic agents.", "content": "(1) Amide local anaesthetics are almost completely metabolized before excretion in both animals and man. (2) Considerable interspecies variability occurs in the quantitative excretion of local anaesthetic metabolites; however, qualitative similarities often exist. (3) The secondary amine metabolite of lignocaine, MEGX, is capable of reacting in vivo with acetaldehyde, formed as a metabolite of ethyl alcohol, to produce a cyclic condensation product. (4) Although hydrolysis in man of the secondary amine metabolite of lignocaine, MEGX, appears extensive, the same is not true for the metabolic analogue produced from mepivacaine, PPX. (5) Metabolism of mepivacaine by the neonate is impaired, but the excretion process in the newborn is capable of eliminating the drug within 24 hr after birth. (6) Metabolic data on bupivacaine and etidocaine are incomplete. The latter compound appears to have a more rapid plasma clearance.", "contents": "A review of the metabolism of amide local anaesthetic agents. (1) Amide local anaesthetics are almost completely metabolized before excretion in both animals and man. (2) Considerable interspecies variability occurs in the quantitative excretion of local anaesthetic metabolites; however, qualitative similarities often exist. (3) The secondary amine metabolite of lignocaine, MEGX, is capable of reacting in vivo with acetaldehyde, formed as a metabolite of ethyl alcohol, to produce a cyclic condensation product. (4) Although hydrolysis in man of the secondary amine metabolite of lignocaine, MEGX, appears extensive, the same is not true for the metabolic analogue produced from mepivacaine, PPX. (5) Metabolism of mepivacaine by the neonate is impaired, but the excretion process in the newborn is capable of eliminating the drug within 24 hr after birth. (6) Metabolic data on bupivacaine and etidocaine are incomplete. The latter compound appears to have a more rapid plasma clearance."}
{"id": "PMID:1096920", "title": "Cardiovascular pharmacology of local anaesthetics.", "content": "Although most local anaesthetic drugs are generally regarded as vasodilators at their site of injection, they produce systemic effects characterized by increases in peripheral resistance, cardiac output, arterial pressure heart rate. The direct depressive effects of the drugs are counterbalanced by increased sympathetic activity. There is evidence that many local anaesthetic agents increase the myogenic tone of certain vascular beds. A proper understanding of these phenomena could lead to improvements in the development of new local anaesthetic agents and appropriate vasoconstrictors.", "contents": "Cardiovascular pharmacology of local anaesthetics. Although most local anaesthetic drugs are generally regarded as vasodilators at their site of injection, they produce systemic effects characterized by increases in peripheral resistance, cardiac output, arterial pressure heart rate. The direct depressive effects of the drugs are counterbalanced by increased sympathetic activity. There is evidence that many local anaesthetic agents increase the myogenic tone of certain vascular beds. A proper understanding of these phenomena could lead to improvements in the development of new local anaesthetic agents and appropriate vasoconstrictors."}
{"id": "PMID:1096921", "title": "Cardiovascular effects of extradural anaesthesia.", "content": "The changes that occur in the circulation following extradural block are described and compared with spinal anaesthesia. The contribution of height of blockade, the effects of the local anaesthesic agent after absorption and the modification in the haemodynamic changes caused by vasopressors added to the local anaesthetic agent, are discussed.", "contents": "Cardiovascular effects of extradural anaesthesia. The changes that occur in the circulation following extradural block are described and compared with spinal anaesthesia. The contribution of height of blockade, the effects of the local anaesthesic agent after absorption and the modification in the haemodynamic changes caused by vasopressors added to the local anaesthetic agent, are discussed."}
{"id": "PMID:1096922", "title": "Intercostal nerve blockade for evaluation of local anaesthetic agents.", "content": "Bilateral intercostal nerve block provides the opportunity to subject as many as 16 separate peripheral nerves in a single subject to known or unknown local anaesthetic agents in a variety of concentrations, volumes, and additives. It permits the observation of local (e.g., neuritis), clinical (e.g., onset and duration), and systemic (e.g., toxicity and blood concentration) effects of these variables. In double-blind studies, bilateral intercostal nerve block allows the use of each side of the trunk for comparison of two experimental drugs, a new drug against a standard, or two new drugs. Subtle differences in clinical properties as well as simultaneous blood concentrations may be detected in these studies. The advantages of this technique in evaluating local anaesthetic agents are primarily the use of a single subject as his own control while studying may separate peripheral nerves. This aids appreciably in limiting the variable of age, temperature, and perfusion, as well as techniques of administration and evaluation. The constancy of the anatomy of the intercostal nerve provides a highly reliable and reproducible block technique.", "contents": "Intercostal nerve blockade for evaluation of local anaesthetic agents. Bilateral intercostal nerve block provides the opportunity to subject as many as 16 separate peripheral nerves in a single subject to known or unknown local anaesthetic agents in a variety of concentrations, volumes, and additives. It permits the observation of local (e.g., neuritis), clinical (e.g., onset and duration), and systemic (e.g., toxicity and blood concentration) effects of these variables. In double-blind studies, bilateral intercostal nerve block allows the use of each side of the trunk for comparison of two experimental drugs, a new drug against a standard, or two new drugs. Subtle differences in clinical properties as well as simultaneous blood concentrations may be detected in these studies. The advantages of this technique in evaluating local anaesthetic agents are primarily the use of a single subject as his own control while studying may separate peripheral nerves. This aids appreciably in limiting the variable of age, temperature, and perfusion, as well as techniques of administration and evaluation. The constancy of the anatomy of the intercostal nerve provides a highly reliable and reproducible block technique."}
{"id": "PMID:1096923", "title": "Double-blind controlled crossover high-dose study of Azaribine in psoriasis.", "content": "Azaribine used in high doses of 200 mg/kg a day is an effective agent in inducing temporary remissions in patients with severe psoriasis but potentially serious neurotoxicity may occur. Therapy should be initiated at lower doses of 125 mg/kg a day and advanced carefully only if clinical response does not ensue at the lower level.", "contents": "Double-blind controlled crossover high-dose study of Azaribine in psoriasis. Azaribine used in high doses of 200 mg/kg a day is an effective agent in inducing temporary remissions in patients with severe psoriasis but potentially serious neurotoxicity may occur. Therapy should be initiated at lower doses of 125 mg/kg a day and advanced carefully only if clinical response does not ensue at the lower level."}
{"id": "PMID:1096924", "title": "Dermal-epidermal deposition of complement components and properdin in systemic lupus erythematosus.", "content": "Skin lesions and clinically normal skin of thirteen patients with systemic lupus erythematosus (SLE) were examined, by the use of immunofluorescent techniques, to determine the presence of CIq, C3, C3 proactivator (C3PA), properdin, immunoglobulins (IgG, IgA, and IgM), and fibrin. IgM deposition was present in all thirteen skin lesions and in eleven normal areas tested, whereas IgG deposition occurred in eleven of the lesions but in only six normal areas. IgA was the least frequently encountered immunoglobulin. CIq deposition occurred in all thirteen skin lesions, and C3 deposition was present in twelve. Seven of nine and eight of eleven clinically normal areas demonstrated CIq and C3 deposition, respectively. Although less intense that CIq and C3 deposition, properdin deposition occurred in eight of the thirteen skin lesions tested but in only two of nine normal areas. C3PA deposition was of greater intensity than was properdin, but was seen in only five lesions and three clinically normal areas. Seven skin lesions and two normal areas also demonstrated fibrin deposition. Although alternate pathway activation is apparent, our findings suggest that the classical pathway (CI to C9) is the primary complement pathway involved in SLE skin.", "contents": "Dermal-epidermal deposition of complement components and properdin in systemic lupus erythematosus. Skin lesions and clinically normal skin of thirteen patients with systemic lupus erythematosus (SLE) were examined, by the use of immunofluorescent techniques, to determine the presence of CIq, C3, C3 proactivator (C3PA), properdin, immunoglobulins (IgG, IgA, and IgM), and fibrin. IgM deposition was present in all thirteen skin lesions and in eleven normal areas tested, whereas IgG deposition occurred in eleven of the lesions but in only six normal areas. IgA was the least frequently encountered immunoglobulin. CIq deposition occurred in all thirteen skin lesions, and C3 deposition was present in twelve. Seven of nine and eight of eleven clinically normal areas demonstrated CIq and C3 deposition, respectively. Although less intense that CIq and C3 deposition, properdin deposition occurred in eight of the thirteen skin lesions tested but in only two of nine normal areas. C3PA deposition was of greater intensity than was properdin, but was seen in only five lesions and three clinically normal areas. Seven skin lesions and two normal areas also demonstrated fibrin deposition. Although alternate pathway activation is apparent, our findings suggest that the classical pathway (CI to C9) is the primary complement pathway involved in SLE skin."}
{"id": "PMID:1096925", "title": "Growth inhibition in normal mammalian melanocytes in vitro.", "content": "Studies on primary cultures of normal adult melanocytes from the ear skin of a black strain of guinea-pigs are reported. Melanocytes have a low proliferation rate and tend to form cohesive groups on a glass surface. The cells undergo division while maintaining close contacts with each other whereas DNA synthesis is rare in melanocytes with multiple contacts.", "contents": "Growth inhibition in normal mammalian melanocytes in vitro. Studies on primary cultures of normal adult melanocytes from the ear skin of a black strain of guinea-pigs are reported. Melanocytes have a low proliferation rate and tend to form cohesive groups on a glass surface. The cells undergo division while maintaining close contacts with each other whereas DNA synthesis is rare in melanocytes with multiple contacts."}
{"id": "PMID:1096926", "title": "The junctional zone beneath migrating epidermis.", "content": "The changes that take palce in skin explants in vitro at the interface between migrating epidermal cells and dermis have been examined by light and electron microscopy and by immunofluorescent antibody techniques. In the first 24 hr epidermal cell migration occurred in the absence of any of the usual components of this junctional zone. After 48 hr of incubation antigenic determinants of the basal zone were detected and basal lamina was demonstrated ultrastructurally. It is proposed that migrating epidermal cells do not need a complex and specialized junctional zone and that these cells are themselves responsible for the elaboration of new basal lamina.", "contents": "The junctional zone beneath migrating epidermis. The changes that take palce in skin explants in vitro at the interface between migrating epidermal cells and dermis have been examined by light and electron microscopy and by immunofluorescent antibody techniques. In the first 24 hr epidermal cell migration occurred in the absence of any of the usual components of this junctional zone. After 48 hr of incubation antigenic determinants of the basal zone were detected and basal lamina was demonstrated ultrastructurally. It is proposed that migrating epidermal cells do not need a complex and specialized junctional zone and that these cells are themselves responsible for the elaboration of new basal lamina."}
{"id": "PMID:1096927", "title": "High dose ascorbic acid in the management of thalassaemia leg ulcers--a pilot study.", "content": "Eight patients with beta thalassaemia major suffering from leg ulcers, were treated over an 8-week period with 3 g ascorbic acid daily in a controlled double-blind crossover study. The ulcers of all the patients showed a high rate of either complete or partial healing.", "contents": "High dose ascorbic acid in the management of thalassaemia leg ulcers--a pilot study. Eight patients with beta thalassaemia major suffering from leg ulcers, were treated over an 8-week period with 3 g ascorbic acid daily in a controlled double-blind crossover study. The ulcers of all the patients showed a high rate of either complete or partial healing."}
{"id": "PMID:1096928", "title": "Oral retinoic acid as therapy for congenital ichthyosiform erythroderma.", "content": "Oral retinoic acid has been therapeutically beneficial for three patients with congenital ichthyosiform erythroderma and one with congenital bullous ichthyosifrom erythroderma. Response to therapy was slow but definite with suppression of scale formation, marked reduction of painful cracking of the underlying epidermis and disappearance of skin irritation. Side effects have not occurred. Therapy had to be maintained to prevent recurrence of symptoms.", "contents": "Oral retinoic acid as therapy for congenital ichthyosiform erythroderma. Oral retinoic acid has been therapeutically beneficial for three patients with congenital ichthyosiform erythroderma and one with congenital bullous ichthyosifrom erythroderma. Response to therapy was slow but definite with suppression of scale formation, marked reduction of painful cracking of the underlying epidermis and disappearance of skin irritation. Side effects have not occurred. Therapy had to be maintained to prevent recurrence of symptoms."}
{"id": "PMID:1096930", "title": "The effect of a low calorie diet or a thiazide diuretic on the incidence of pre-eclampsia and on birth weight.", "content": "A 1200 calorie diet or cyclopenthiazide with potassium was given to two groups of 51 high weight gain primigravidae and baby weight and the incidence of pre-eclampsia were compared with those in a matched control group.  There was no difference in the development of pre-eclampsia, but the weight of babies in the control group was higher than in the treated groups.  The body fat was reduced in the diet group and the total body water in the diuretic group.", "contents": "The effect of a low calorie diet or a thiazide diuretic on the incidence of pre-eclampsia and on birth weight. A 1200 calorie diet or cyclopenthiazide with potassium was given to two groups of 51 high weight gain primigravidae and baby weight and the incidence of pre-eclampsia were compared with those in a matched control group.  There was no difference in the development of pre-eclampsia, but the weight of babies in the control group was higher than in the treated groups.  The body fat was reduced in the diet group and the total body water in the diuretic group."}
{"id": "PMID:1096931", "title": "Oral malignant melanoma.", "content": "Sixty-five cases of oral malignant melanoma are reviewed, comprising 53 cases taken from the literature and 12 unreported cases.  The classification of tumours on clinical and histopathological grounds is suggested to be comparable to that for cutaneous melanoma.", "contents": "Oral malignant melanoma. Sixty-five cases of oral malignant melanoma are reviewed, comprising 53 cases taken from the literature and 12 unreported cases.  The classification of tumours on clinical and histopathological grounds is suggested to be comparable to that for cutaneous melanoma."}
{"id": "PMID:1096932", "title": "Sequence homology of the mitochondrial leucyl-tRNA cistron in different organisms.", "content": "Sequence divergence of the mitochondrial leucytl-tRNA cistron in several eukaryotes has been examined by RNA-DNA hybridized. Rat mitochondria Leucyl-tRNA was hybridized with rat, mouse, guinea pig, monkey, chicken, and yeast mitochondrial DNAs (mtDNA) immobilized onfilters. Hybridization was carried out in 50% formamide (Tm -12degrees) or in 20% fromamide (Tm -21degrees). melting profiles of the hybrids were obtained for evaluation of the extent of base sequence micmatching. Under the more stringent hybridization conditions (50% formamide, Tm -12degrees), only mouse and quines pig mtDNAs hybridized with rat mitochondrial leucyl-tRNA. The Tm's of the heterohybrids were depressed by 2 and 9 degrees, respectively. Under less stringent hybridization conditions (Tm-21 degrees), monkey mtDNA also hybridized, and the Tm was depressed by about 15 degrees. Chicken and yeast mtDNAs did not form specific hybrids with rat mibochondrial leucyl-tRNA under these hybridization conditions. Mitochondrial leucyl-tRNA sequences in different eukaryotes appear to be conserved to a less extent than cytoplasmic rRNA, 5S RNA, or hemoglobin mRNA sequences.", "contents": "Sequence homology of the mitochondrial leucyl-tRNA cistron in different organisms. Sequence divergence of the mitochondrial leucytl-tRNA cistron in several eukaryotes has been examined by RNA-DNA hybridized. Rat mitochondria Leucyl-tRNA was hybridized with rat, mouse, guinea pig, monkey, chicken, and yeast mitochondrial DNAs (mtDNA) immobilized onfilters. Hybridization was carried out in 50% formamide (Tm -12degrees) or in 20% fromamide (Tm -21degrees). melting profiles of the hybrids were obtained for evaluation of the extent of base sequence micmatching. Under the more stringent hybridization conditions (50% formamide, Tm -12degrees), only mouse and quines pig mtDNAs hybridized with rat mitochondrial leucyl-tRNA. The Tm's of the heterohybrids were depressed by 2 and 9 degrees, respectively. Under less stringent hybridization conditions (Tm-21 degrees), monkey mtDNA also hybridized, and the Tm was depressed by about 15 degrees. Chicken and yeast mtDNAs did not form specific hybrids with rat mibochondrial leucyl-tRNA under these hybridization conditions. Mitochondrial leucyl-tRNA sequences in different eukaryotes appear to be conserved to a less extent than cytoplasmic rRNA, 5S RNA, or hemoglobin mRNA sequences."}
{"id": "PMID:1096933", "title": "The interaction of heavy meromyosin and subfragment 1 with actin. Physical measurements in the presence and absence of adenosine triphosphate.", "content": "Viscosity, turbidity, and laser-light fluctuation autocorrelations of acto-heavy merymyosin (HMM) and acto-subfragment 1 (S-1) solutions were measured under conditions where the actin-activated ATPase is close to its maximal value. The results were compared to similar data obtained in the absence of ATP where the actin and myosin fragments were completely domplexed, and in the presence of ATP but at 0.1 M KLC where the actin and HMM or S-1 were almost completely dissociated. It was found that at maximal actin activation, the viscosity, turbidity, and autocorrelation data were all much closer to the values for the completely dissociated systems than to the values for the completely complexed systems. Assuming that viscosity, turbidity, and autocorrelation measurements approximate a linear measure of binding between actin and HMM or S-1, the results suggest that at maximal actin activation less than 10% of the HMM or S-1 are bound to the actin. Therefore as was suggested previously by ultracentrifuge and kinetics studies, it appears that under conditions of maximal actin activation, most of the HMM and S-1 occur in a refractory state unable to bind to actin.", "contents": "The interaction of heavy meromyosin and subfragment 1 with actin. Physical measurements in the presence and absence of adenosine triphosphate. Viscosity, turbidity, and laser-light fluctuation autocorrelations of acto-heavy merymyosin (HMM) and acto-subfragment 1 (S-1) solutions were measured under conditions where the actin-activated ATPase is close to its maximal value. The results were compared to similar data obtained in the absence of ATP where the actin and myosin fragments were completely domplexed, and in the presence of ATP but at 0.1 M KLC where the actin and HMM or S-1 were almost completely dissociated. It was found that at maximal actin activation, the viscosity, turbidity, and autocorrelation data were all much closer to the values for the completely dissociated systems than to the values for the completely complexed systems. Assuming that viscosity, turbidity, and autocorrelation measurements approximate a linear measure of binding between actin and HMM or S-1, the results suggest that at maximal actin activation less than 10% of the HMM or S-1 are bound to the actin. Therefore as was suggested previously by ultracentrifuge and kinetics studies, it appears that under conditions of maximal actin activation, most of the HMM and S-1 occur in a refractory state unable to bind to actin."}
{"id": "PMID:1096934", "title": "Identification of a unique ethidium bromide binding site on yeast tRNAPhe by high resolution (300 MHz) nuclear magnetic resonance.", "content": "The binding of ethidium bromide to yeast tRNAPhe has been studied by high resolution (300 MHz) proton nuclear magnetic resonance. Under appropriate experimental conditions one ethidium bromide is bound to each tRNA and two resonances from ring NH protons are shifted upfield. These observations taken in conjunction with the low-field spectrum of yeast tRNAPhe show that the unique ethidium bromide binding site is located between base pairs AU6 and AU7 of the amino acid acceptor stem. This information should be of value in understanding the way in which ethidium bromide binding alters the biochemical properties of the tRNA molecules.", "contents": "Identification of a unique ethidium bromide binding site on yeast tRNAPhe by high resolution (300 MHz) nuclear magnetic resonance. The binding of ethidium bromide to yeast tRNAPhe has been studied by high resolution (300 MHz) proton nuclear magnetic resonance. Under appropriate experimental conditions one ethidium bromide is bound to each tRNA and two resonances from ring NH protons are shifted upfield. These observations taken in conjunction with the low-field spectrum of yeast tRNAPhe show that the unique ethidium bromide binding site is located between base pairs AU6 and AU7 of the amino acid acceptor stem. This information should be of value in understanding the way in which ethidium bromide binding alters the biochemical properties of the tRNA molecules."}
{"id": "PMID:1096935", "title": "Kinetic evidence for the obligatory formation of a 30S initiation complex in polyphenylalanine synthesis initiated with N-acetylphenylalanyl-5RNA.", "content": "The problem of whether the initiation of bacterial protein synthesis involves the obligatory formation of a 30S initiation complex intermediate was examined in a model system with N-acetylphenylalanyl-tRNA as initiator 5RNA nad poly(uridylic acid) as mRNA. The time courses of the formation of the 30S and 70S initiation complex with Escherichia coli ribosomes were measured simultaneously by stopping the reaction with dextran sulfate and differentiating the N-acetylphenylalanyl-tRNA bound to 30S ribosomal subunits from that bound to 70S ribosomes with RNase I, which hydrolyzes N-acetylphenylalanyl-tRNA bound to 30S subunits but not that bound to 70S ribosomes. A maximum in the 30S complex concentration was observed within the first 10-15 sec of the reaction, whereas 70S complex formed formed more slowly with a slight initial time lag. When an analog computer was programmed with rate constants determined separately for the formation of the 30S initiation complex from preformed 30S complex, kinetic curves very similar to the empirical curves were obtained for the entire time course of the reaction. The results show clearly that formation of the 70S complex obeys the kinetic laws for consecutive reactions, and the 30S complex is, therefore, an obligatory intermediate in the initiation of polyphenylalanine synthesis in the model system.", "contents": "Kinetic evidence for the obligatory formation of a 30S initiation complex in polyphenylalanine synthesis initiated with N-acetylphenylalanyl-5RNA. The problem of whether the initiation of bacterial protein synthesis involves the obligatory formation of a 30S initiation complex intermediate was examined in a model system with N-acetylphenylalanyl-tRNA as initiator 5RNA nad poly(uridylic acid) as mRNA. The time courses of the formation of the 30S and 70S initiation complex with Escherichia coli ribosomes were measured simultaneously by stopping the reaction with dextran sulfate and differentiating the N-acetylphenylalanyl-tRNA bound to 30S ribosomal subunits from that bound to 70S ribosomes with RNase I, which hydrolyzes N-acetylphenylalanyl-tRNA bound to 30S subunits but not that bound to 70S ribosomes. A maximum in the 30S complex concentration was observed within the first 10-15 sec of the reaction, whereas 70S complex formed formed more slowly with a slight initial time lag. When an analog computer was programmed with rate constants determined separately for the formation of the 30S initiation complex from preformed 30S complex, kinetic curves very similar to the empirical curves were obtained for the entire time course of the reaction. The results show clearly that formation of the 70S complex obeys the kinetic laws for consecutive reactions, and the 30S complex is, therefore, an obligatory intermediate in the initiation of polyphenylalanine synthesis in the model system."}
{"id": "PMID:1096936", "title": "The isolation and characterization of nuclear ghosts from cultured HeLa cells.", "content": "Macromolecular complexes, which appear as ghosts when viewed by phase contrast microscopy, have been isolated from the nuclei of HeLa cells grown in culture. The preparation of these ghosts involves a detergent wash which removes the unit membranes of the nuclear envelop structure but leaves intact both the nuclear pores and the dense structure conferring nuclear margins (possibly the dense lamella). Detergent-washed nuclei are subsequently treated with 0.5 M MgCl2 and fractionated on continuous sucrose gradients containing 0.5 M MgCl2. The ghosts are recovered as a sharp band at an apparent sucrose density of 47-52% and consist of 72% protein, 10% phospholipid, 14% DNA, And 4% RNA. The release of the majority of intranuclear components is indicated by the large loss of nuclear DNA (95%), RNA (71%), and protein (87%) contrasted to the small loss of phospholipid (27%) druing the conversion of detergent washed nuclei to isolated ghosts. Sodium dodecyl sulfate-polyacrylamide gel patterns of the ghost proteins consist of two major bands with approximate molecular weights of 20,000 and 35,000. The isolation of ghosts with a similar density and protein composition from nondetergent-washed nuclei indicates that the ghost is not an artifact induced by the detergent treatment. The absence of cytoplasmic contamination in the preparations of detergent washed nuclei and nuclear ghosts was demonstrated by chemical, enzymatic, and electron microscope studies. We suggest that the isolated ghosts represent a structural macromolecular complex which underlies and is probably attached to the inner nuclear membrane of intact nuclei. The possible additional presence of intranuclear network proteins has not been excluded.", "contents": "The isolation and characterization of nuclear ghosts from cultured HeLa cells. Macromolecular complexes, which appear as ghosts when viewed by phase contrast microscopy, have been isolated from the nuclei of HeLa cells grown in culture. The preparation of these ghosts involves a detergent wash which removes the unit membranes of the nuclear envelop structure but leaves intact both the nuclear pores and the dense structure conferring nuclear margins (possibly the dense lamella). Detergent-washed nuclei are subsequently treated with 0.5 M MgCl2 and fractionated on continuous sucrose gradients containing 0.5 M MgCl2. The ghosts are recovered as a sharp band at an apparent sucrose density of 47-52% and consist of 72% protein, 10% phospholipid, 14% DNA, And 4% RNA. The release of the majority of intranuclear components is indicated by the large loss of nuclear DNA (95%), RNA (71%), and protein (87%) contrasted to the small loss of phospholipid (27%) druing the conversion of detergent washed nuclei to isolated ghosts. Sodium dodecyl sulfate-polyacrylamide gel patterns of the ghost proteins consist of two major bands with approximate molecular weights of 20,000 and 35,000. The isolation of ghosts with a similar density and protein composition from nondetergent-washed nuclei indicates that the ghost is not an artifact induced by the detergent treatment. The absence of cytoplasmic contamination in the preparations of detergent washed nuclei and nuclear ghosts was demonstrated by chemical, enzymatic, and electron microscope studies. We suggest that the isolated ghosts represent a structural macromolecular complex which underlies and is probably attached to the inner nuclear membrane of intact nuclei. The possible additional presence of intranuclear network proteins has not been excluded."}
{"id": "PMID:1096937", "title": "Incorporation of fluorotryptophans into proteins of escherichia coli.", "content": "A tryptophan-requiring strain of Escherichia coli can go through two doublings of optical density after L-tryptophan is replaced in the medium by 4-fluorotryptophan, during which the fluoro analog displaces approximately 75% of the L-tryptophan in cell protein. One doubling occurs in the presence of 5- or 6-fluorotryptophan, with 50-60% replacement of L-tryptophan by analog. When beta-galactosidase is induced at the time of addition of analog, it reaches 60% of the control specific activity in the presence of 4-fluorotryptophan, 10% of normal in the presence of 5- or 6-fluorotryptophan. Lactose permease activity is 35% of the control in the presence of 4- and 6-fluorotryptophan, less than 10% in the presence of 5-fluorotryptophan. D-Lactate dehydrogenase shows a specific activity twice that of the control in the presence of 4-fluorotryptophan, one-half with 5- or 6-fluorotryptophan. Thus fluorotryptophan can be incorporated into proteins and affect their activities, although the nature and magnitude of the effect cannot be predicted for any given enzyme. Such substituted proteins should be useful for the study of protein structure and function by 19F nuclear magnetic resonance and other techniques.", "contents": "Incorporation of fluorotryptophans into proteins of escherichia coli. A tryptophan-requiring strain of Escherichia coli can go through two doublings of optical density after L-tryptophan is replaced in the medium by 4-fluorotryptophan, during which the fluoro analog displaces approximately 75% of the L-tryptophan in cell protein. One doubling occurs in the presence of 5- or 6-fluorotryptophan, with 50-60% replacement of L-tryptophan by analog. When beta-galactosidase is induced at the time of addition of analog, it reaches 60% of the control specific activity in the presence of 4-fluorotryptophan, 10% of normal in the presence of 5- or 6-fluorotryptophan. Lactose permease activity is 35% of the control in the presence of 4- and 6-fluorotryptophan, less than 10% in the presence of 5-fluorotryptophan. D-Lactate dehydrogenase shows a specific activity twice that of the control in the presence of 4-fluorotryptophan, one-half with 5- or 6-fluorotryptophan. Thus fluorotryptophan can be incorporated into proteins and affect their activities, although the nature and magnitude of the effect cannot be predicted for any given enzyme. Such substituted proteins should be useful for the study of protein structure and function by 19F nuclear magnetic resonance and other techniques."}
{"id": "PMID:1096938", "title": "Cooperative interactions in hybrids of aspartate transcarbamylase containing succinylated regulatory polypeptide chains.", "content": "Succinylated derivatives of the regulatory subunit of aspartate transcarbamylase of Escherichia coli were prepared by treating the intact enzyme with succinic anhydride followed by dissociation of the modified protein into catalytic and regulatory subunits which were separated by ion exchange chromatography. The succinylated regulatory subunits were used in hybridization experiments with native subunits to study the organization of the six regulatory polypeptide chains in the intact enzyme. Rapid mixing of succinylated and native regulatory subunits with native catalytic subunits yielded a four-membered hybrid set of reconstituted enzyme-like molecules; hence, the assembly process involves three regulatory combining units and the six regulatory polypeptide chains in the intact enzyme must be arranged as three dimeric subunits. When the modified and native regulatory subunits were incubated together for only brief periods (less than 1 min) followed by the addition of catalytic subunits, the resulting hybrid set was complex with no resolution of discrete species. Apparently the isolated regulatory dimers dissociate readily and reversibly into single polypeptide chains due to relatively weak intra-subunit bonding domains. In contrast, after reconstitution of enzyme-like molecules, the incorporated succinylated regulatory subunits did not exchange with free subunits. Enzyme-like molecules containing three extensively succinylated regulatory subunits show reduced binding of the inhibitor, CTP, and lack both the homotropic and heterotropic effects characteristic of native aspartate transcarbamylase. Preparations containing only slightly succinylated regulatory subunits showed only little inhibition by CTP and considerable cooperativity. The decrease in homotropic effects in these reconstituted molecules correlated with the reduction in the succinate-promoted change in the sedimentation coefficient. Reconstituted enzyme-like molecules containing regulatory subunits which had been extensively succinylated in the presence of CTP retained their binding capacity even though they were only slightly inhibited by CTP and exhibited reduced cooperativity. Hybrid molecules containing both native and succinylated regulatory subunits also possessed reduced allosteric behavior.", "contents": "Cooperative interactions in hybrids of aspartate transcarbamylase containing succinylated regulatory polypeptide chains. Succinylated derivatives of the regulatory subunit of aspartate transcarbamylase of Escherichia coli were prepared by treating the intact enzyme with succinic anhydride followed by dissociation of the modified protein into catalytic and regulatory subunits which were separated by ion exchange chromatography. The succinylated regulatory subunits were used in hybridization experiments with native subunits to study the organization of the six regulatory polypeptide chains in the intact enzyme. Rapid mixing of succinylated and native regulatory subunits with native catalytic subunits yielded a four-membered hybrid set of reconstituted enzyme-like molecules; hence, the assembly process involves three regulatory combining units and the six regulatory polypeptide chains in the intact enzyme must be arranged as three dimeric subunits. When the modified and native regulatory subunits were incubated together for only brief periods (less than 1 min) followed by the addition of catalytic subunits, the resulting hybrid set was complex with no resolution of discrete species. Apparently the isolated regulatory dimers dissociate readily and reversibly into single polypeptide chains due to relatively weak intra-subunit bonding domains. In contrast, after reconstitution of enzyme-like molecules, the incorporated succinylated regulatory subunits did not exchange with free subunits. Enzyme-like molecules containing three extensively succinylated regulatory subunits show reduced binding of the inhibitor, CTP, and lack both the homotropic and heterotropic effects characteristic of native aspartate transcarbamylase. Preparations containing only slightly succinylated regulatory subunits showed only little inhibition by CTP and considerable cooperativity. The decrease in homotropic effects in these reconstituted molecules correlated with the reduction in the succinate-promoted change in the sedimentation coefficient. Reconstituted enzyme-like molecules containing regulatory subunits which had been extensively succinylated in the presence of CTP retained their binding capacity even though they were only slightly inhibited by CTP and exhibited reduced cooperativity. Hybrid molecules containing both native and succinylated regulatory subunits also possessed reduced allosteric behavior."}
{"id": "PMID:1096939", "title": "Proton magnetic resonance studies on the conformation of the hexanucleotide, GmpApApYpApsiP, and Related fragments from the anticodong loop of baker's yeast phenylalanine transfer ribonucleic acid.", "content": "A hexanucleotide, GmpApApYpAppsiP, and 11 related compounds from the anticodon loop of Baker's yeast tRNA-Phe were studied by proton magnetic resonance from 100 to 250 MHz. Totally 19 resonance lines from all the base protons (H8, H6, and H2), H1', and methyl proton resonances of the hexamer have been assigned by a systematic \"incremental procedure\" in comparing all the related shorter fragments. Emphasis is given to the Y base and its stacking conformation with respect to its nearest neighboring bases. The results reveal a strong tendency of the purine bases to have a maximal extent of base-base overlap with their neighbors in the sequence. This tendency is manifested in a zigzag (or balcony-like) mode of base-stacking pattern of the -ApYpA-sequence in the hexamer in which the -pA-residue tends to stack toward the adduct ring (C10, C11, and N12) of Y. This tendency is also shown in the formation of a stack of GmAAA closing the gap left behind by the excision of Y in the hexamer GmpApAp--pApp. The implication of these findings to the structure and function of tRNA is discussed.", "contents": "Proton magnetic resonance studies on the conformation of the hexanucleotide, GmpApApYpApsiP, and Related fragments from the anticodong loop of baker's yeast phenylalanine transfer ribonucleic acid. A hexanucleotide, GmpApApYpAppsiP, and 11 related compounds from the anticodon loop of Baker's yeast tRNA-Phe were studied by proton magnetic resonance from 100 to 250 MHz. Totally 19 resonance lines from all the base protons (H8, H6, and H2), H1', and methyl proton resonances of the hexamer have been assigned by a systematic \"incremental procedure\" in comparing all the related shorter fragments. Emphasis is given to the Y base and its stacking conformation with respect to its nearest neighboring bases. The results reveal a strong tendency of the purine bases to have a maximal extent of base-base overlap with their neighbors in the sequence. This tendency is manifested in a zigzag (or balcony-like) mode of base-stacking pattern of the -ApYpA-sequence in the hexamer in which the -pA-residue tends to stack toward the adduct ring (C10, C11, and N12) of Y. This tendency is also shown in the formation of a stack of GmAAA closing the gap left behind by the excision of Y in the hexamer GmpApAp--pApp. The implication of these findings to the structure and function of tRNA is discussed."}
{"id": "PMID:1096940", "title": "The binding of complementary oligoribonucleotides to yeast initiator Transfer RNA.", "content": "Oligoribonucleotide binding to baker's yeast initiator tRNA was measured by equilibrium dialysis in order to determine which regions of the tRNA were free to bind complementary oligomers and which were involved in secondary and tertiary structure. Association constants of trinucleoside diphosphates and tetranucleoside triphophates complementary to the single-stranded regions of the cloverleaf structure of yeast tRNAfMet were measured at o degrees in 1.0 M NaCl, and 0.01 M MgCl2. The only regions of the tRNA whose complementary oligomers bound to the tRNA were the amino acid acceptor end and the five nucleotides at the 5' end of the anticodon loop. These results differ from those for the other tRNAs studied by this technique; usually oligomers complementary to the dihydrouracil loop bind to the tRNA. The sequence of yeast tRNAfMet and other eucaryotic initiators is unusual. The \"TpsiC loop\" contains the sequence A-U-C instead of T-psi-C, yet the binding pattern to the THE TpsiC LOOP IS LIKE THAT FOR OTHER TRNAs; no oligomers bind.", "contents": "The binding of complementary oligoribonucleotides to yeast initiator Transfer RNA. Oligoribonucleotide binding to baker's yeast initiator tRNA was measured by equilibrium dialysis in order to determine which regions of the tRNA were free to bind complementary oligomers and which were involved in secondary and tertiary structure. Association constants of trinucleoside diphosphates and tetranucleoside triphophates complementary to the single-stranded regions of the cloverleaf structure of yeast tRNAfMet were measured at o degrees in 1.0 M NaCl, and 0.01 M MgCl2. The only regions of the tRNA whose complementary oligomers bound to the tRNA were the amino acid acceptor end and the five nucleotides at the 5' end of the anticodon loop. These results differ from those for the other tRNAs studied by this technique; usually oligomers complementary to the dihydrouracil loop bind to the tRNA. The sequence of yeast tRNAfMet and other eucaryotic initiators is unusual. The \"TpsiC loop\" contains the sequence A-U-C instead of T-psi-C, yet the binding pattern to the THE TpsiC LOOP IS LIKE THAT FOR OTHER TRNAs; no oligomers bind."}
{"id": "PMID:1096941", "title": "Tyrosyl-tRNA synthetase from Escherichia coli. Stoichiometry of ligand binding and half-of-the-sites reactivity in aminoacylation.", "content": "The tyrosyl-tRNA synthetase from Escherichia coli binds only 1 mol of tRNA, tyrosine, and tyrosyl adenylate per mol of enzyme dimer. However, like the enzyme from Bacillus stearothermophilus, once one active site is occupied by tyrosyl adenylate the other becomes accessible to bind a further molecule each of tyrosine and ATP. Both bacterial enzymes show biphasic kinetics with respect to tyrosine in the aminoacylation of tRNA. Equilibrium dialysis experiments show that this is due to 2 mol of tyrosine binding in the presence of ATP and tRNA. A method is given for a correction for the effects of hydrolysis of the charged tRNA on the aminoacylation kinetics.", "contents": "Tyrosyl-tRNA synthetase from Escherichia coli. Stoichiometry of ligand binding and half-of-the-sites reactivity in aminoacylation. The tyrosyl-tRNA synthetase from Escherichia coli binds only 1 mol of tRNA, tyrosine, and tyrosyl adenylate per mol of enzyme dimer. However, like the enzyme from Bacillus stearothermophilus, once one active site is occupied by tyrosyl adenylate the other becomes accessible to bind a further molecule each of tyrosine and ATP. Both bacterial enzymes show biphasic kinetics with respect to tyrosine in the aminoacylation of tRNA. Equilibrium dialysis experiments show that this is due to 2 mol of tyrosine binding in the presence of ATP and tRNA. A method is given for a correction for the effects of hydrolysis of the charged tRNA on the aminoacylation kinetics."}
{"id": "PMID:1096942", "title": "Demonstration of two reaction pathways for the aminoacylation of tRNA. Application of the pulsed quenched flow technique.", "content": "A rapid mixing and quenching device is described which operates efficiently in the range of 150 msec to several minutes as well as the usual time scale of 5-150 msec of the conventional apparatus. This has been used to measure the initial rate of acylation of tRNATyr by the tyrosyl-tRNA synthetase of Escherichia coli during the first turnover of the enzyme, and also the rate constants of the partial reactions of amino acid activation and transfer to the tRNA. It is shown that at saturating conenctration of tRNA the reaction proceeds by a ternary complex mechanism. The rate-determining step is either the aminoacyltion process or a step preceding it. At low concentrations of tRNA the reaction proceeds by the stepwise process of formation of tyrosyl adenylate followed by acylation of the tRNA. The rate constants for these partial reactions are faster than that for the ternary complex reaction. But the prior binding of tRNA greatly decreases the rate of tyrosyl adenylate formation. Both pathways are probably important at physiological concentrations. 88% of the tyrosine from the tyrosyl adenylate complex is transferred to tRNA. The presence of added tyrosine and ATP reduces this to 78%. However, the addition of aliquots of ATP to a mixture of enzyme, tyrosine, and a saturating concentration of tRNA (i.e., ternary complex conditions) leads to at least 0.97 mol of tRNA being acylated/mol of ATP hydrolyzed. Trapping experiments show that the 12% of adenylated that is not transferred to tRNA is hydrolyzed on the enzyme rather than expelled into solution.", "contents": "Demonstration of two reaction pathways for the aminoacylation of tRNA. Application of the pulsed quenched flow technique. A rapid mixing and quenching device is described which operates efficiently in the range of 150 msec to several minutes as well as the usual time scale of 5-150 msec of the conventional apparatus. This has been used to measure the initial rate of acylation of tRNATyr by the tyrosyl-tRNA synthetase of Escherichia coli during the first turnover of the enzyme, and also the rate constants of the partial reactions of amino acid activation and transfer to the tRNA. It is shown that at saturating conenctration of tRNA the reaction proceeds by a ternary complex mechanism. The rate-determining step is either the aminoacyltion process or a step preceding it. At low concentrations of tRNA the reaction proceeds by the stepwise process of formation of tyrosyl adenylate followed by acylation of the tRNA. The rate constants for these partial reactions are faster than that for the ternary complex reaction. But the prior binding of tRNA greatly decreases the rate of tyrosyl adenylate formation. Both pathways are probably important at physiological concentrations. 88% of the tyrosine from the tyrosyl adenylate complex is transferred to tRNA. The presence of added tyrosine and ATP reduces this to 78%. However, the addition of aliquots of ATP to a mixture of enzyme, tyrosine, and a saturating concentration of tRNA (i.e., ternary complex conditions) leads to at least 0.97 mol of tRNA being acylated/mol of ATP hydrolyzed. Trapping experiments show that the 12% of adenylated that is not transferred to tRNA is hydrolyzed on the enzyme rather than expelled into solution."}
{"id": "PMID:1096943", "title": "Fragments of bovine serum albumin produced by limited proteolysis. Isolation and characterization of tryptic fragments.", "content": "Several fragments of bovine serum albumin have been isolated following limited tryptic hydrolysis and their positions then determined in the bovine serum albumin sequence published by J. R. Brown ((1975), Fed. Proc., Fed. Am. Soc. Exp. Biol. 34, 591). When bovine serum albumin was coupled to palmityl-aminoethylamino-agarose and digested with trypsin, two fragments were obtained: (a) peptide 115-184, containing the highly aromatic disulfide loop 3 of Brown's model, and (b) a larger fragment, residues 377-581, containing disulfide loops 7-9. This fragment constitutes the third of the three domains of the albumin molecule. From bovine serum albumin digested in solution, peptide 115-184 was again obtained, as well as (c) a 39,000-dalton fragment identified as residues 198-581, loops 4-9 of the second and third domains, but with a long, tryptophan-containing segment 204-238 missing from loop 4. The ability to isolate these fragments without cleaving disulfide bridges is partial confirmation of the proposed model of bovine serum albumin as a series of nine independent loops.", "contents": "Fragments of bovine serum albumin produced by limited proteolysis. Isolation and characterization of tryptic fragments. Several fragments of bovine serum albumin have been isolated following limited tryptic hydrolysis and their positions then determined in the bovine serum albumin sequence published by J. R. Brown ((1975), Fed. Proc., Fed. Am. Soc. Exp. Biol. 34, 591). When bovine serum albumin was coupled to palmityl-aminoethylamino-agarose and digested with trypsin, two fragments were obtained: (a) peptide 115-184, containing the highly aromatic disulfide loop 3 of Brown's model, and (b) a larger fragment, residues 377-581, containing disulfide loops 7-9. This fragment constitutes the third of the three domains of the albumin molecule. From bovine serum albumin digested in solution, peptide 115-184 was again obtained, as well as (c) a 39,000-dalton fragment identified as residues 198-581, loops 4-9 of the second and third domains, but with a long, tryptophan-containing segment 204-238 missing from loop 4. The ability to isolate these fragments without cleaving disulfide bridges is partial confirmation of the proposed model of bovine serum albumin as a series of nine independent loops."}
{"id": "PMID:1096944", "title": "Difference in pyruvate kinase regulation among three groups of yeasts.", "content": "Yeast pyruvate kinase (ATP : pyruvate 2-O-phosphotransferase EC 2.7.1.40) was classified into three groups based on the interaction with fructose-1,6-bisphosphate. The pyruvate kinases of Saccharomyces cerevisiae and Saccharomyces carlsbergensis were activated by fructose 1,6-bisphosphate in the concentration range tested (up to 10 mM) of the substrate, phosphoenolpyruvate; the enzymes of \"fermentative Candida\" (Candida tropicalis and Candida utilis) were affected by fructose 1,6-biphosphate only when the substrate concentration was below 2 mM. Although the pyruvate kinase of Candida lipolytica (a yeast belonging to \"oxidative Candida\") was also affected by fructose 1,6-bisphosphate, the degree of the activation was extremely small as compared with the above four yeasts. The pyruvate kinase of C. tropicalis was inhibited by ATP more strongly in the absence of fructose 1,6-bisphosphate than its presence. In the case of the C. lipolytica enzyme, however, the enzyme was inhibited to a lesser extent by ATP, and fructose 1,6-bisphosphate did not reverse the inhibitory effect of ATP. Time course changes of the enzyme levels in the yeasts grown on glucose and on ethanol indicate that the pyruvate kinases of S. cerevisiae and C. tropicalis can be controlled both by an allosteric mechanism and by changes in the enzyme concentration, although a marked difference was observed in the susceptibility to the allosteric effect by fructose 1,6-biphosphate  between these fermentative yeasts. On the other hand, that of C. lipolytica would be controlled only by the latter mechanism.", "contents": "Difference in pyruvate kinase regulation among three groups of yeasts. Yeast pyruvate kinase (ATP : pyruvate 2-O-phosphotransferase EC 2.7.1.40) was classified into three groups based on the interaction with fructose-1,6-bisphosphate. The pyruvate kinases of Saccharomyces cerevisiae and Saccharomyces carlsbergensis were activated by fructose 1,6-bisphosphate in the concentration range tested (up to 10 mM) of the substrate, phosphoenolpyruvate; the enzymes of \"fermentative Candida\" (Candida tropicalis and Candida utilis) were affected by fructose 1,6-biphosphate only when the substrate concentration was below 2 mM. Although the pyruvate kinase of Candida lipolytica (a yeast belonging to \"oxidative Candida\") was also affected by fructose 1,6-bisphosphate, the degree of the activation was extremely small as compared with the above four yeasts. The pyruvate kinase of C. tropicalis was inhibited by ATP more strongly in the absence of fructose 1,6-bisphosphate than its presence. In the case of the C. lipolytica enzyme, however, the enzyme was inhibited to a lesser extent by ATP, and fructose 1,6-bisphosphate did not reverse the inhibitory effect of ATP. Time course changes of the enzyme levels in the yeasts grown on glucose and on ethanol indicate that the pyruvate kinases of S. cerevisiae and C. tropicalis can be controlled both by an allosteric mechanism and by changes in the enzyme concentration, although a marked difference was observed in the susceptibility to the allosteric effect by fructose 1,6-biphosphate  between these fermentative yeasts. On the other hand, that of C. lipolytica would be controlled only by the latter mechanism."}
{"id": "PMID:1096945", "title": "Isolation, chemical and immunological characterization of two strongly basic nuclear proteins from human spermatozoa.", "content": "Human spermatozoa were solubilized by treatment with urea/guanidine and fractionated by ion-exchange chromatography with Bio-Rex 70 resin. Four fractions were obtained. The acidic proteins pass unretarded, whereas the moderately basic and two strongly basic protein fractions are eluted by means of guanidine gradients. The main protein components of the strongly basic protein fractions have been isolated by gel filtration on Sephadex G-50. The purified proteins have been named human sperm protamine 1 and 2. They contain 47 and 51 aminoacid residues (mol. wt 6280 and 6840), of which 22 and 24 are arginine and 5 and 4 are half cystine residues, respectively. The electrophoretic mobility in urea/polyacrylamide gels is between that of calf thymus histone and salmon protamine. Human protamine 1 and 2 are both auto-antigens as has been detected by a quantitative immunofluorescence inhibition test.", "contents": "Isolation, chemical and immunological characterization of two strongly basic nuclear proteins from human spermatozoa. Human spermatozoa were solubilized by treatment with urea/guanidine and fractionated by ion-exchange chromatography with Bio-Rex 70 resin. Four fractions were obtained. The acidic proteins pass unretarded, whereas the moderately basic and two strongly basic protein fractions are eluted by means of guanidine gradients. The main protein components of the strongly basic protein fractions have been isolated by gel filtration on Sephadex G-50. The purified proteins have been named human sperm protamine 1 and 2. They contain 47 and 51 aminoacid residues (mol. wt 6280 and 6840), of which 22 and 24 are arginine and 5 and 4 are half cystine residues, respectively. The electrophoretic mobility in urea/polyacrylamide gels is between that of calf thymus histone and salmon protamine. Human protamine 1 and 2 are both auto-antigens as has been detected by a quantitative immunofluorescence inhibition test."}
{"id": "PMID:1096946", "title": "Determination of UTP and ATP pool sizes in human tonsillar lymphocytes by using Escherichia coli RNA polymerase.", "content": "The present paper describes a rapid, specific and sensitive method for quantitating ribonucleoside triphosphates (ATP and UTP) in cell extracts. The principle of the method is based on the synthesis of a ribonucleotide polymer in the presence of UTP, ATP and poly(dA-dT) as template. A method for calculation is also described, making the determination of UTP and ATP pool sizes in the cells possible under the same experimental conditions. The calculation takes into account the isotope dilution effect caused by the intracellular ATP. Our experiments show that the neutralized perchloric acid soluble fraction of human tonsillar lymphocytes contains no inhibitors for the RNA polymerase test. According to our results, this cell extract contains 80 pmol of UTP and 340 pmol of ATP per mug RNA.", "contents": "Determination of UTP and ATP pool sizes in human tonsillar lymphocytes by using Escherichia coli RNA polymerase. The present paper describes a rapid, specific and sensitive method for quantitating ribonucleoside triphosphates (ATP and UTP) in cell extracts. The principle of the method is based on the synthesis of a ribonucleotide polymer in the presence of UTP, ATP and poly(dA-dT) as template. A method for calculation is also described, making the determination of UTP and ATP pool sizes in the cells possible under the same experimental conditions. The calculation takes into account the isotope dilution effect caused by the intracellular ATP. Our experiments show that the neutralized perchloric acid soluble fraction of human tonsillar lymphocytes contains no inhibitors for the RNA polymerase test. According to our results, this cell extract contains 80 pmol of UTP and 340 pmol of ATP per mug RNA."}
{"id": "PMID:1096947", "title": "Analysis of the breaking sites in the physical degradation of DNA.", "content": "The 3'-termini of procaryotic and eucaryotic DNA fragments obtained by shearing, sonication and irradiation have been analyzed. Analysis of the 3'-ends of DNA fragments was done by 32-P-labeling with terminal transferase, hydrolysis of products by spleen DNAase and spleen exonuclease and separation of labeled 3'-terminal nucleotides on polyethyleneimine plates. In the case of sonication the deviation from DNA base composition of 3'-terminal nucleotides is very close to random for Escherichia coli and Haemophilius influenzae DNA, whereas for calf thymus, mouse and yeast mitochondrial DNAs a significant increase in dA and decrease in dC was observed. Shearing at high salt released random 3'-termini in E. coli DNA and 3'-termini with 4 to 6% deviation from base composition in calf thymus DNA. In the case of gamma-irradiation no real differences have been found between E. coli and calf thymus DNA and 3'-terminal nucleotide composition is very close to random. Mechanical breakage of eucaryotic DNA seems to release 3'-termini having a composition which differs slightly but significantly from the average base composition of the DNA.", "contents": "Analysis of the breaking sites in the physical degradation of DNA. The 3'-termini of procaryotic and eucaryotic DNA fragments obtained by shearing, sonication and irradiation have been analyzed. Analysis of the 3'-ends of DNA fragments was done by 32-P-labeling with terminal transferase, hydrolysis of products by spleen DNAase and spleen exonuclease and separation of labeled 3'-terminal nucleotides on polyethyleneimine plates. In the case of sonication the deviation from DNA base composition of 3'-terminal nucleotides is very close to random for Escherichia coli and Haemophilius influenzae DNA, whereas for calf thymus, mouse and yeast mitochondrial DNAs a significant increase in dA and decrease in dC was observed. Shearing at high salt released random 3'-termini in E. coli DNA and 3'-termini with 4 to 6% deviation from base composition in calf thymus DNA. In the case of gamma-irradiation no real differences have been found between E. coli and calf thymus DNA and 3'-terminal nucleotide composition is very close to random. Mechanical breakage of eucaryotic DNA seems to release 3'-termini having a composition which differs slightly but significantly from the average base composition of the DNA."}
{"id": "PMID:1096948", "title": "Physiological transition of a coliphage lambda DNA replication.", "content": "The \"rolling-circle\" replicative intermediate (sigma-type molecules) which is normally produced in the late stage of coliphage lambda DNA replication can be found during the first round of lambda DNA replication if cells infected with lambda replication mutant Ots28 are incubated at the nonpermissive temperature until the late stage of the latent period of lambda infection. After shifting to the permissive temperature, the vast majority of replicating forms are sigma-type rolling circle even during the first round of DNA replication. Concatemeric lambda DNA molecules, produced from these sigma-type intermediates, are efficiently packaged into progeny phage, indicating that in the first round of lambda DNA replication, double-branched theta-type molecules are not required for production of viable progeny phage.", "contents": "Physiological transition of a coliphage lambda DNA replication. The \"rolling-circle\" replicative intermediate (sigma-type molecules) which is normally produced in the late stage of coliphage lambda DNA replication can be found during the first round of lambda DNA replication if cells infected with lambda replication mutant Ots28 are incubated at the nonpermissive temperature until the late stage of the latent period of lambda infection. After shifting to the permissive temperature, the vast majority of replicating forms are sigma-type rolling circle even during the first round of DNA replication. Concatemeric lambda DNA molecules, produced from these sigma-type intermediates, are efficiently packaged into progeny phage, indicating that in the first round of lambda DNA replication, double-branched theta-type molecules are not required for production of viable progeny phage."}
{"id": "PMID:1096949", "title": "The mode of action of griseoviridin at the ribosome level.", "content": "The antibiotic griseoviridin binds to the larger subunit of Escherichia coli ribosomes blocking the interaction between the 3' terminal end of peptidyl-tRNA and the donor site of the peptidyl transferase centre. Griseoviridin inhibits binding of chloramphenicol, thiamphenicol, lincomycin, erythromycin and streptogramin A to bacterial ribosomes. Moreover griseoviridin protects the ribosomal binding site of gougerotin from the drastic conformational changes taking place in the presence of ethanol. Griseoviridin is also able to interact with eukaryotic ribosomes as shown by its effects on  model systems and on anisomycin, trichodermin and gougerotin binding studies. Nevertheless, griseoviridin affinity for the 80-S type ribosomes (yeast or human) is two orders of magnitude smaller than with E. coli ribosomes. The inhibitory spectrum and mode of action of griseoviridin on ribosomes is compared to that of antibiotics of the streptogramin A group and found to be essentially the same.", "contents": "The mode of action of griseoviridin at the ribosome level. The antibiotic griseoviridin binds to the larger subunit of Escherichia coli ribosomes blocking the interaction between the 3' terminal end of peptidyl-tRNA and the donor site of the peptidyl transferase centre. Griseoviridin inhibits binding of chloramphenicol, thiamphenicol, lincomycin, erythromycin and streptogramin A to bacterial ribosomes. Moreover griseoviridin protects the ribosomal binding site of gougerotin from the drastic conformational changes taking place in the presence of ethanol. Griseoviridin is also able to interact with eukaryotic ribosomes as shown by its effects on  model systems and on anisomycin, trichodermin and gougerotin binding studies. Nevertheless, griseoviridin affinity for the 80-S type ribosomes (yeast or human) is two orders of magnitude smaller than with E. coli ribosomes. The inhibitory spectrum and mode of action of griseoviridin on ribosomes is compared to that of antibiotics of the streptogramin A group and found to be essentially the same."}
{"id": "PMID:1096950", "title": "Bacteriophage phi X174 DNA synthesis in Escherichia coli HF4704S (dnaHts) cells.", "content": "The DNA synthesis of bacteriophage phiX174 in Escherichia coli HF470S, a mutant temperature sensitive in the initiation of DNA replication (dnaHts), has been examined. In HF4704S cells, phiX174 can grow normally at 27 degree C whereas the phage cannot grow after the cessation of DNA synthesis of the host cells at 42 degrees C. Upon infection, phiX174 DNA can be injected into the host cell and the parental replicative form can be formed, but the progency replicative form cannot be synthesized at 43 degrees C in the absence of host DNA synthesis. The progency replicative form cannot be synthesized at 27 degrees C in the presence of 30 mug chloramphenicol/ml in the host cell which has been incubated for 74 min at 43 degrees C followed by transfer to 27 degrees C in the presence of 30 mug chloramphenicol/ml. When 30 mug chloramphenicol/ml is added later than 5 min after the temperature shift-down to 27 degrees C, the progency replicative form synthesis is not inhibited. Thus, the host cell function, for which the gene dnaH is responsible, has been shown to be essential to the progency replicative form production.", "contents": "Bacteriophage phi X174 DNA synthesis in Escherichia coli HF4704S (dnaHts) cells. The DNA synthesis of bacteriophage phiX174 in Escherichia coli HF470S, a mutant temperature sensitive in the initiation of DNA replication (dnaHts), has been examined. In HF4704S cells, phiX174 can grow normally at 27 degree C whereas the phage cannot grow after the cessation of DNA synthesis of the host cells at 42 degrees C. Upon infection, phiX174 DNA can be injected into the host cell and the parental replicative form can be formed, but the progency replicative form cannot be synthesized at 43 degrees C in the absence of host DNA synthesis. The progency replicative form cannot be synthesized at 27 degrees C in the presence of 30 mug chloramphenicol/ml in the host cell which has been incubated for 74 min at 43 degrees C followed by transfer to 27 degrees C in the presence of 30 mug chloramphenicol/ml. When 30 mug chloramphenicol/ml is added later than 5 min after the temperature shift-down to 27 degrees C, the progency replicative form synthesis is not inhibited. Thus, the host cell function, for which the gene dnaH is responsible, has been shown to be essential to the progency replicative form production."}
{"id": "PMID:1096951", "title": "The ATP-dependent DNAase from Escherichia coli rorA: a nuclease with changed enzymatic properties.", "content": "The ATP-dependent DNAases from Escherichia coli wild-type and rorA were isolated and purified and their enzymatic properties were compared. The enzymes were found to differ in the amount of ATP that is consumed during DNA degradation. This difference can be influenced by the reaction conditions and the nature of the substrate.", "contents": "The ATP-dependent DNAase from Escherichia coli rorA: a nuclease with changed enzymatic properties. The ATP-dependent DNAases from Escherichia coli wild-type and rorA were isolated and purified and their enzymatic properties were compared. The enzymes were found to differ in the amount of ATP that is consumed during DNA degradation. This difference can be influenced by the reaction conditions and the nature of the substrate."}
{"id": "PMID:1096952", "title": "Partial degradation of transfer RNAs by different preparations of snake venom exonuclease.", "content": "The degradation of yeast tRNASer with eight different exonuclease preparations from four snake venoms was investigated. The reaction products were separated on polyacrylamide gels containing 7 M urea. Patterns of sharp bands were obtained which were more or less similar. Two tRNA fragments were characterized by oligonucleotide analyses, one of which was tRNA degraded by the exonuclease up to the beginning of the T-phi-C-stem. The other one was generated by the additional loss of several nucleotides from the 5'-terminus. The formation of the latter fragment was very probably caused by an endonuclease activity in the exonuclease. The endonuclease contaminant, which was found in all preparations, was further investigated by experiments with modified tRNAs whose 3'-terminus should be resistant to exonuclease (tRNASer-A, tRNASerOX-red). With 3'-AMP as substrate no phosphatase activity was found under the conditions of tRNA degradation. Not only in tRNASer, but also in yeast tRNATyr and tRNAAla as well as in fragments of tRNASer and tRNAPhe, the degradation by exonuclease was inhibited at the beginning of the T-phi-C-stem. The finding of such a retardation site in addition to the general retardation of exonuclease digestion after removal of the C-C-A sequence may indicate that retardation at certain elements of secondary structure is a more general feature of degradations by this enzyme.", "contents": "Partial degradation of transfer RNAs by different preparations of snake venom exonuclease. The degradation of yeast tRNASer with eight different exonuclease preparations from four snake venoms was investigated. The reaction products were separated on polyacrylamide gels containing 7 M urea. Patterns of sharp bands were obtained which were more or less similar. Two tRNA fragments were characterized by oligonucleotide analyses, one of which was tRNA degraded by the exonuclease up to the beginning of the T-phi-C-stem. The other one was generated by the additional loss of several nucleotides from the 5'-terminus. The formation of the latter fragment was very probably caused by an endonuclease activity in the exonuclease. The endonuclease contaminant, which was found in all preparations, was further investigated by experiments with modified tRNAs whose 3'-terminus should be resistant to exonuclease (tRNASer-A, tRNASerOX-red). With 3'-AMP as substrate no phosphatase activity was found under the conditions of tRNA degradation. Not only in tRNASer, but also in yeast tRNATyr and tRNAAla as well as in fragments of tRNASer and tRNAPhe, the degradation by exonuclease was inhibited at the beginning of the T-phi-C-stem. The finding of such a retardation site in addition to the general retardation of exonuclease digestion after removal of the C-C-A sequence may indicate that retardation at certain elements of secondary structure is a more general feature of degradations by this enzyme."}
{"id": "PMID:1096953", "title": "30 S pre-ribosomal RNA of Escherichia coli:primary and secondary processing.", "content": "The metabolism of newly-formed labeled 30 S pre-ribosomal RNA was studied in the Escherichia coli mutant strain AB301-105. Detailed kinetic analysis in rifampicin-treated cells showed that precursors of 23 S and 16 S rRNA are formed from the 30 S RNA species, even in phosphate-limited cultures. To establish the order fo segments along 30 S pre-rRNA, it was allowed to accumulate in replicate chloramphenicol-treated cultures, and pulse-labeled after the addition of rifampicin in segments that progressively contained more 3'-distal label with time. The purified RNAs from the various cultures were then cleaved to a limited extent by RNAase III; the specific activity of the fragments yielded an order from the 5'-end of 17.5S-X-25S, where X refers to some of the sequences released as additional small fragments. More extensive treatment the 25-S and 17.5-S pre-rRNA chains with RNAase III yielded additional fragments and produced RNA species with the mobility of the 23-S and 16-S RNA precursors made in normal cells treated with chloramphenicol. A processing scheme is suggested that distinguishes between early, site-specific cleavage of recognition sites in the RNA itself ('primary processing'), and later, \"secondary processing' reactions. The secondary processing reactions are blocked chloramphenicol-treated wild-type cells.", "contents": "30 S pre-ribosomal RNA of Escherichia coli:primary and secondary processing. The metabolism of newly-formed labeled 30 S pre-ribosomal RNA was studied in the Escherichia coli mutant strain AB301-105. Detailed kinetic analysis in rifampicin-treated cells showed that precursors of 23 S and 16 S rRNA are formed from the 30 S RNA species, even in phosphate-limited cultures. To establish the order fo segments along 30 S pre-rRNA, it was allowed to accumulate in replicate chloramphenicol-treated cultures, and pulse-labeled after the addition of rifampicin in segments that progressively contained more 3'-distal label with time. The purified RNAs from the various cultures were then cleaved to a limited extent by RNAase III; the specific activity of the fragments yielded an order from the 5'-end of 17.5S-X-25S, where X refers to some of the sequences released as additional small fragments. More extensive treatment the 25-S and 17.5-S pre-rRNA chains with RNAase III yielded additional fragments and produced RNA species with the mobility of the 23-S and 16-S RNA precursors made in normal cells treated with chloramphenicol. A processing scheme is suggested that distinguishes between early, site-specific cleavage of recognition sites in the RNA itself ('primary processing'), and later, \"secondary processing' reactions. The secondary processing reactions are blocked chloramphenicol-treated wild-type cells."}
{"id": "PMID:1096954", "title": "Purification of biosynthetic threonine deaminase from Escherichia coli.", "content": "Biosynthetic threonine deaminase (L-threonine hydro-lyase (deaminating), EC 4.2.1.16) was purified to apparent homogeneity from cell extracts of Escherichia coli by chromatographic procedures using valine-Sepharose, isoleucine-N-hexamethyleneamine-Sepharose, and hydroxyapatite with an overall yield of 40%. Analytical ultracentrifugation shows a molecular weight of 214 000. In sodium dodecyl sulfate gel electrophoresis, the enzyme migrates as a single band corresponding to a molecular weight of about 50 000. These data confirm that the enzyme is a tetramer. The sedimentation coefficient, s-020,w, determined by differential sedimentation experiments is 9.2 S. The enzyme shows absorption maxima at 415 and 280 nm. Determination of pyridoxal phosphate by three indenpendent methods shows the presence of two molecules of pyridoxal phosphate per enzyme molecule, the different methods being in excellent agreement equilibrium dialysis experiments establish the presence of two isoleucine binding sites. The Scatchard plot suggests non-cooperativity of these sites. The association constant for isoleucine is 1.2 - 10(5)M-1.", "contents": "Purification of biosynthetic threonine deaminase from Escherichia coli. Biosynthetic threonine deaminase (L-threonine hydro-lyase (deaminating), EC 4.2.1.16) was purified to apparent homogeneity from cell extracts of Escherichia coli by chromatographic procedures using valine-Sepharose, isoleucine-N-hexamethyleneamine-Sepharose, and hydroxyapatite with an overall yield of 40%. Analytical ultracentrifugation shows a molecular weight of 214 000. In sodium dodecyl sulfate gel electrophoresis, the enzyme migrates as a single band corresponding to a molecular weight of about 50 000. These data confirm that the enzyme is a tetramer. The sedimentation coefficient, s-020,w, determined by differential sedimentation experiments is 9.2 S. The enzyme shows absorption maxima at 415 and 280 nm. Determination of pyridoxal phosphate by three indenpendent methods shows the presence of two molecules of pyridoxal phosphate per enzyme molecule, the different methods being in excellent agreement equilibrium dialysis experiments establish the presence of two isoleucine binding sites. The Scatchard plot suggests non-cooperativity of these sites. The association constant for isoleucine is 1.2 - 10(5)M-1."}
{"id": "PMID:1096955", "title": "Anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyl-transferase from Salmonella typhimurium. Inactivation of glutamine-dependent anthranilate synthetase by agarose-bound anthranilate.", "content": "Exposure of the anthranilate synthetase-anthranilate phosphoribosyltransferase enzyme complex (chorismate pyruvate-lyase (amino-accepting) EC 4.1.3.27 and N-(5-phosphoribosyl)-anthranilate pyrophosphate phosphoribosyl-transferase, EC 2.4.2.18) from Salmonella typhimurium to agarose-bound anthranilate led to the slow inactivation of glutamine-dependent anthranilate synthetase activity, an activity dependent on protein-protein interaction in the enzyme complex. Region I of phosphoribosyltransferase, the location of the enzyme complex glutaminase activity, is the site of alteration. Phosphoribosyltransferase and NH3-dependent anthranilate synthetase activities and trypto phan regulation of phosphoribosyltransferase were unaffected by the anthranilate matrix. The molecular weight (280 000) and subunit molecular weight (62 000) of the enzyme complex eluted from an anthranilate matrix were identical to those of enzyme complex purified by classical methodology. The enzyme complex could be partially protected against inactivation by storiing in 0.1 M L-glutamine or 30% glycerol and completely protected by storing in 50% glycerol at -18 degrees C. Evidence is presented that the anthranilate matrix acts as a hydrophobic matrix and may be binding to and altering a hydrophobic region in the enzyme complex. The anthranilate matrix provides a convenient tool for altering a specific region of an enzyme complex without covalent modification. At the same time, the results demonstrate that affinity matrices are not necessarily innocuous but may subject macromolecules to an adverse environment not previously recognized.", "contents": "Anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyl-transferase from Salmonella typhimurium. Inactivation of glutamine-dependent anthranilate synthetase by agarose-bound anthranilate. Exposure of the anthranilate synthetase-anthranilate phosphoribosyltransferase enzyme complex (chorismate pyruvate-lyase (amino-accepting) EC 4.1.3.27 and N-(5-phosphoribosyl)-anthranilate pyrophosphate phosphoribosyl-transferase, EC 2.4.2.18) from Salmonella typhimurium to agarose-bound anthranilate led to the slow inactivation of glutamine-dependent anthranilate synthetase activity, an activity dependent on protein-protein interaction in the enzyme complex. Region I of phosphoribosyltransferase, the location of the enzyme complex glutaminase activity, is the site of alteration. Phosphoribosyltransferase and NH3-dependent anthranilate synthetase activities and trypto phan regulation of phosphoribosyltransferase were unaffected by the anthranilate matrix. The molecular weight (280 000) and subunit molecular weight (62 000) of the enzyme complex eluted from an anthranilate matrix were identical to those of enzyme complex purified by classical methodology. The enzyme complex could be partially protected against inactivation by storiing in 0.1 M L-glutamine or 30% glycerol and completely protected by storing in 50% glycerol at -18 degrees C. Evidence is presented that the anthranilate matrix acts as a hydrophobic matrix and may be binding to and altering a hydrophobic region in the enzyme complex. The anthranilate matrix provides a convenient tool for altering a specific region of an enzyme complex without covalent modification. At the same time, the results demonstrate that affinity matrices are not necessarily innocuous but may subject macromolecules to an adverse environment not previously recognized."}
{"id": "PMID:1096956", "title": "Isolation and initial characterization of glutathione-deficient mutants of Escherichia coli K 12.", "content": "The thiol-oxidizing agent \"diamide\" (CH3)2NCON equal to NCON(CH3)2 was used to isolate mutants of Escherichia coli K 12 deficient in the biosynthesis of glutathione. A colony-colour technique has been developed for identification of colonies of these mutants. Four glutathione-deficient mutants were isolated. They show normal growth rates in minimal medium without GSH supplementation, indicating that glutathione is not involved in essential metabolic process. In one mutant, glutathione synthetase was entirely inactive. Three mutants were deficient in gamma-glutamylcysteine synthetase; in two of them, this resulted in a complete lack of GSH. These mutants were found to be more susceptible than their parent strains to a wide rang of chemical agents, but did not show a greater sensitivity to X-rays. It must be concluded that the protective role of glutathione is only significant when a chemical challenge is present.", "contents": "Isolation and initial characterization of glutathione-deficient mutants of Escherichia coli K 12. The thiol-oxidizing agent \"diamide\" (CH3)2NCON equal to NCON(CH3)2 was used to isolate mutants of Escherichia coli K 12 deficient in the biosynthesis of glutathione. A colony-colour technique has been developed for identification of colonies of these mutants. Four glutathione-deficient mutants were isolated. They show normal growth rates in minimal medium without GSH supplementation, indicating that glutathione is not involved in essential metabolic process. In one mutant, glutathione synthetase was entirely inactive. Three mutants were deficient in gamma-glutamylcysteine synthetase; in two of them, this resulted in a complete lack of GSH. These mutants were found to be more susceptible than their parent strains to a wide rang of chemical agents, but did not show a greater sensitivity to X-rays. It must be concluded that the protective role of glutathione is only significant when a chemical challenge is present."}
{"id": "PMID:1096957", "title": "Type III procollagen and collagen in skin.", "content": "A form of collagen, containing three alpha chains of type III, can be extracted from foetal calf, calf and rat skin under physiological conditions. This native collagen was purified by DEAE-cellulose chromatography and then was analysed by polyacrylamide gel electrophoresis which showed it consisted of several high molecular weight components, the size of gamma components and larger species. Prior reduction in dithiothreitol dissociated these large polymers into two components: the minor one migrated between the alpha1 (I) and alpha2 chains while the predominant one migrated between the alpha and beta chains. These two monomers were isolated by CM-cellulose chromatography. The minor one, which eluted between the alpha1 and alpha2 chains, had a molecular weight of approx. 95 000; its amino acid composition was similar to that of alpha1(III). The major one eluted in the alpha1 region and had a molecular weight of approx. 120 000; its amino acid composition, while similar to that of the alpha1(III) chain, differed in detail, and it is presumed to be a pro-alpha1(III) chain. Following pepsin digestion, the native collagen remained as a disulfide-bonded trimer which dissociated into only one component, a1(III), when denatured in dithiothreitol. These results suggest that the original, extracted protein consisted primarily ofa precursor form of type III collagen. This procollagen did not polymerize when heated at 37 degrees C and did not form the usual segment long spacing aggregates under suitable conditions. It was not modified by incubation with a purified procollagen peptidase preparation. This appears to be the first example of the isolation of type III (pro)collagen by extractive methods, without resorting to tissue digestion by proteolytic enzymes.", "contents": "Type III procollagen and collagen in skin. A form of collagen, containing three alpha chains of type III, can be extracted from foetal calf, calf and rat skin under physiological conditions. This native collagen was purified by DEAE-cellulose chromatography and then was analysed by polyacrylamide gel electrophoresis which showed it consisted of several high molecular weight components, the size of gamma components and larger species. Prior reduction in dithiothreitol dissociated these large polymers into two components: the minor one migrated between the alpha1 (I) and alpha2 chains while the predominant one migrated between the alpha and beta chains. These two monomers were isolated by CM-cellulose chromatography. The minor one, which eluted between the alpha1 and alpha2 chains, had a molecular weight of approx. 95 000; its amino acid composition was similar to that of alpha1(III). The major one eluted in the alpha1 region and had a molecular weight of approx. 120 000; its amino acid composition, while similar to that of the alpha1(III) chain, differed in detail, and it is presumed to be a pro-alpha1(III) chain. Following pepsin digestion, the native collagen remained as a disulfide-bonded trimer which dissociated into only one component, a1(III), when denatured in dithiothreitol. These results suggest that the original, extracted protein consisted primarily ofa precursor form of type III collagen. This procollagen did not polymerize when heated at 37 degrees C and did not form the usual segment long spacing aggregates under suitable conditions. It was not modified by incubation with a purified procollagen peptidase preparation. This appears to be the first example of the isolation of type III (pro)collagen by extractive methods, without resorting to tissue digestion by proteolytic enzymes."}
{"id": "PMID:1096958", "title": "Reversibility of the affinity labelled-biotin transport system in yeast cells.", "content": "Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotynyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling  of the transport system.", "contents": "Reversibility of the affinity labelled-biotin transport system in yeast cells. Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotynyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling  of the transport system."}
{"id": "PMID:1096959", "title": "Proteins of the camel erythrocyte membrane.", "content": "Electrophoresis on polyacrylamide gels containing dodecyl sulphate has revealed that the major proteins of the camel erythrocyte membrane are similar to those of the human and bovine species in both electrophoretic mobility and relative abundance. The major difference lies in the major intrinsic membrane protein of molecular weight approx. 100 000. In the camel, this protein has a higher apparent molecular weight than in the human and bovine species. The very high molecular weight water-soluble protein \"spectrin\" appears to be very tightly bound to the camel erythrocyte membrane, and is only partially extracted after prolonged low ionic strength dialysis. Total release of spectrin is only achieved by means of more drastic treatment such as incubation with urea, guanidine hydrochloride, sodium hydroxide of p-chloromercuribenzoate. Concurrent with the total release of spectrin, the camel cells undergo a shape change from flat ellipsoids to spheres, suggesting an important shape-maintaining role for spectrin in the erythrocytes of this species.", "contents": "Proteins of the camel erythrocyte membrane. Electrophoresis on polyacrylamide gels containing dodecyl sulphate has revealed that the major proteins of the camel erythrocyte membrane are similar to those of the human and bovine species in both electrophoretic mobility and relative abundance. The major difference lies in the major intrinsic membrane protein of molecular weight approx. 100 000. In the camel, this protein has a higher apparent molecular weight than in the human and bovine species. The very high molecular weight water-soluble protein \"spectrin\" appears to be very tightly bound to the camel erythrocyte membrane, and is only partially extracted after prolonged low ionic strength dialysis. Total release of spectrin is only achieved by means of more drastic treatment such as incubation with urea, guanidine hydrochloride, sodium hydroxide of p-chloromercuribenzoate. Concurrent with the total release of spectrin, the camel cells undergo a shape change from flat ellipsoids to spheres, suggesting an important shape-maintaining role for spectrin in the erythrocytes of this species."}
{"id": "PMID:1096964", "title": "[Individuality of mannonate and altronate hydro-lyases in Escherichia coli K 12].", "content": "In Escherichia coli, mannonic and altronic hydrolyases act, respectively, on mannonate, the intermediate aldonate of the glucuronate branch, and on altronate the intermediate aldonate of the galacturonate branch of the hexuronate pathway, yielding 2-keto-3-deoxy-gluconate. Our results demonstrate that the two hydrolyases are two distinct proteins. First, each hydrolyase shows a different induction pattern. In addition, separate constitutive mutants for either hydrolyase have been obtained. Second, single mutants negatively affected for one of the activities but not the other have been isolated in each case. Third, comparative heat inactivation of both activities at 59 degrees C shows mannonic hydrolyase to be clearly more thermosensitive than altronic hydrolyase. Furthermore the two enzymes also react differently to various effectors. Fourth, the two enzymes could be resolved on a DEAE cellulose column into two neighbouring but distinct peaks of activity, and a further purification yielded two pure hydrolyase fractions each being devoid of the activity of the other.", "contents": "[Individuality of mannonate and altronate hydro-lyases in Escherichia coli K 12]. In Escherichia coli, mannonic and altronic hydrolyases act, respectively, on mannonate, the intermediate aldonate of the glucuronate branch, and on altronate the intermediate aldonate of the galacturonate branch of the hexuronate pathway, yielding 2-keto-3-deoxy-gluconate. Our results demonstrate that the two hydrolyases are two distinct proteins. First, each hydrolyase shows a different induction pattern. In addition, separate constitutive mutants for either hydrolyase have been obtained. Second, single mutants negatively affected for one of the activities but not the other have been isolated in each case. Third, comparative heat inactivation of both activities at 59 degrees C shows mannonic hydrolyase to be clearly more thermosensitive than altronic hydrolyase. Furthermore the two enzymes also react differently to various effectors. Fourth, the two enzymes could be resolved on a DEAE cellulose column into two neighbouring but distinct peaks of activity, and a further purification yielded two pure hydrolyase fractions each being devoid of the activity of the other."}
{"id": "PMID:1096966", "title": "[Purification of beta-lactamases by affinity chromatography].", "content": "Affinity columns able to purifie beta-lactamases have been prepared either by linking covalently reversible inhibitors or substrates to agarose beds. The enzyme is eluted with a gradient of sodium chloride or released with the substrate. This method is a pertinent one for the purification of these enzymes and for the study of bacteria harbouring more than one beta-lactamase.", "contents": "[Purification of beta-lactamases by affinity chromatography]. Affinity columns able to purifie beta-lactamases have been prepared either by linking covalently reversible inhibitors or substrates to agarose beds. The enzyme is eluted with a gradient of sodium chloride or released with the substrate. This method is a pertinent one for the purification of these enzymes and for the study of bacteria harbouring more than one beta-lactamase."}
{"id": "PMID:1096962", "title": "[Polarization state of the light source and determination of attenuated total internal reflection spectra in intact microorganisms].", "content": "The problems are considered of the interaction of linear polarized light current with undestroyed microorganisms while recording the spectra of attenuated total refraction(ATR) and repeated ATR (RATR). It is shown that proper allowance should be made of dichrosim of absorption bands of microorganisms during quantitative studies. Peculiarities of obtaining RATR spectra of the linearly polarized light source with polarization azinut other than 0 and 90 degrees are considered.", "contents": "[Polarization state of the light source and determination of attenuated total internal reflection spectra in intact microorganisms]. The problems are considered of the interaction of linear polarized light current with undestroyed microorganisms while recording the spectra of attenuated total refraction(ATR) and repeated ATR (RATR). It is shown that proper allowance should be made of dichrosim of absorption bands of microorganisms during quantitative studies. Peculiarities of obtaining RATR spectra of the linearly polarized light source with polarization azinut other than 0 and 90 degrees are considered."}
{"id": "PMID:1096963", "title": "[Determination of the optical constants of intact microogranisms by the method of attenuated internal reflection spectroscopy].", "content": "The problems are considered of the determination of the optical constants (dispersion of refraction, n2, and absorption, K, indexes) of strongly dispersing biological objects on undestroyed cells of microorganisms (E. coli, strain C-85) by the method of repeated attenuated total refraction. The volume of unpacked intercellular space and the effect of repeated propagation of the light current through measuring elements with high refraction index were taken into account. Relationships R, s (n1, n2, O) K were obtained for determining the optical constants on the elements with the angle of light current incidence 0=45 degrees, made of infrared glass, IRG-24, IRF-24 and of Si and Ge as well. The optical constants of bacteria in the region of absorption bands 1660, 1550 cm-1 were also determined.", "contents": "[Determination of the optical constants of intact microogranisms by the method of attenuated internal reflection spectroscopy]. The problems are considered of the determination of the optical constants (dispersion of refraction, n2, and absorption, K, indexes) of strongly dispersing biological objects on undestroyed cells of microorganisms (E. coli, strain C-85) by the method of repeated attenuated total refraction. The volume of unpacked intercellular space and the effect of repeated propagation of the light current through measuring elements with high refraction index were taken into account. Relationships R, s (n1, n2, O) K were obtained for determining the optical constants on the elements with the angle of light current incidence 0=45 degrees, made of infrared glass, IRG-24, IRF-24 and of Si and Ge as well. The optical constants of bacteria in the region of absorption bands 1660, 1550 cm-1 were also determined."}
{"id": "PMID:1096967", "title": "tRNAs undermethylation in a met-regulatory mutant of Saccharomyces cerevisiae.", "content": "A study of in vivo and in vitro methylation of tRNAs in regulatory mutants affected in methionine-mediated repression (eth2, eth3, eth10) has led to the following results: 1) The eth2-2 carrying strain presents a great undermethylation of its tRNAs of the same order of magnitude as observed during methionine starvation of methionine auxotrophs. 2) This undermethylation leads to a shift of the tRNAIII met peak on a BD cellulose column, while tRNAIII met peak is unchanged. 3) The study of a double mutant strain carrying eth2 and met2 mutations has shown that this undermethylation is a consequence of the high internal pool of methionine. 4) Undermethylation unequally affects the different bases and the different tRNA species.", "contents": "tRNAs undermethylation in a met-regulatory mutant of Saccharomyces cerevisiae. A study of in vivo and in vitro methylation of tRNAs in regulatory mutants affected in methionine-mediated repression (eth2, eth3, eth10) has led to the following results: 1) The eth2-2 carrying strain presents a great undermethylation of its tRNAs of the same order of magnitude as observed during methionine starvation of methionine auxotrophs. 2) This undermethylation leads to a shift of the tRNAIII met peak on a BD cellulose column, while tRNAIII met peak is unchanged. 3) The study of a double mutant strain carrying eth2 and met2 mutations has shown that this undermethylation is a consequence of the high internal pool of methionine. 4) Undermethylation unequally affects the different bases and the different tRNA species."}
{"id": "PMID:1096968", "title": "Sequence diagrams and the presentation of structural and evolutionary relationships among proteins.", "content": "Protein sequences mapped on two-dimensional diagrams show characteristic patterns that should be of value in visualising sequence information and in distinguishing simpler structures. A convenient map form for comparative purposes is the alpha-helix diagram with aminoacid distribution analogous to the surface of an alpha-helix oriented so that an alpha-helix structure corresponds on the diagram to a vertical band 3.6 residues wide. The sequence diagram for an alpha-keratin, high-sulphur protein suggests a new form of polypeptide helix based on a repeating unit of five which may be an important component of alpha-keratin fibres.", "contents": "Sequence diagrams and the presentation of structural and evolutionary relationships among proteins. Protein sequences mapped on two-dimensional diagrams show characteristic patterns that should be of value in visualising sequence information and in distinguishing simpler structures. A convenient map form for comparative purposes is the alpha-helix diagram with aminoacid distribution analogous to the surface of an alpha-helix oriented so that an alpha-helix structure corresponds on the diagram to a vertical band 3.6 residues wide. The sequence diagram for an alpha-keratin, high-sulphur protein suggests a new form of polypeptide helix based on a repeating unit of five which may be an important component of alpha-keratin fibres."}
{"id": "PMID:1096970", "title": "The application of nuclear magnetic resonance spectroscopy to the study of natural and model membranes.", "content": "This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of individual chemical groups in lipid bilayer regions of natural and model membranes, through the determination of physical parameters like chemical shifts, spin-lattice (T1) and spin-spin (T2) nuclear magnetic relaxation times. The results of some significant experiments carried out on single-wall lecithin vesicles as well as on intact natural membranes, are summarized and discussed. Useful information can be obtained on the lipid fatty-acid chains thermal transition, by comparing two lecithin vesicles of the same size, formed by the same host lecithin, but incorporated with different molecular components. In particular, T1 and T2 measurements, interpreted in terms of a two- (or more-) correlation time theoretical models, are able to demonstrate different degrees of motional anisotropy in bilayers formed by mixed lecithins or by mixtures of lecithin and fatty acids, possessing moderately different chain lengths [13]. Chromophore-containing molecules, like chlorophyll [12] or fluorescent probes [14] can be located, within few Angstroms, in a lipid bilayer through proton chemical shift measurements; in addition the perturbation of the lipid membrane structure, as induced by the incorporated probe, is assessed mainly in terms of the intramolecular dynamical structure of the host lecithin molecules, by means of T1 and linewidth studies. The comparison of the n.m.r. relaxation behaviour in intact membranes and in vesicles formed by their extracted lipids may, finally, provide indirect information on the lipid-protein intermolecular interactions and relative mobility, besides indicating the intramolecular mobility characters of the lipid bilayer regions of the membrane.", "contents": "The application of nuclear magnetic resonance spectroscopy to the study of natural and model membranes. This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of individual chemical groups in lipid bilayer regions of natural and model membranes, through the determination of physical parameters like chemical shifts, spin-lattice (T1) and spin-spin (T2) nuclear magnetic relaxation times. The results of some significant experiments carried out on single-wall lecithin vesicles as well as on intact natural membranes, are summarized and discussed. Useful information can be obtained on the lipid fatty-acid chains thermal transition, by comparing two lecithin vesicles of the same size, formed by the same host lecithin, but incorporated with different molecular components. In particular, T1 and T2 measurements, interpreted in terms of a two- (or more-) correlation time theoretical models, are able to demonstrate different degrees of motional anisotropy in bilayers formed by mixed lecithins or by mixtures of lecithin and fatty acids, possessing moderately different chain lengths [13]. Chromophore-containing molecules, like chlorophyll [12] or fluorescent probes [14] can be located, within few Angstroms, in a lipid bilayer through proton chemical shift measurements; in addition the perturbation of the lipid membrane structure, as induced by the incorporated probe, is assessed mainly in terms of the intramolecular dynamical structure of the host lecithin molecules, by means of T1 and linewidth studies. The comparison of the n.m.r. relaxation behaviour in intact membranes and in vesicles formed by their extracted lipids may, finally, provide indirect information on the lipid-protein intermolecular interactions and relative mobility, besides indicating the intramolecular mobility characters of the lipid bilayer regions of the membrane."}
{"id": "PMID:1096971", "title": "Lymphoreticular aggregates in the newborn lung and their relation to intrauterine INFECTION and neurospinal dysraphism.", "content": "A survey has been carried out of lymphoreticular aggregates in the lungs of 200 children, stillborns and dying within 15 days of birth and in a further 21 children with anencephalus, and controls. Lymphoreticular aggregates occur in response to amniotic infection and are found in the 'intrauterine infection syndrome'. They are not found in children showing early hyaline membranes. The time interval required for the production of lymphoreticular aggregates in the human child appears to be at lease 48 h and is probably 72 h before the production of well-formed aggregates and this probably applies to both the intrauterine and the postnatal state. There is a significant increase in the number of lungs showing lymphoreticular aggregates at birth in children in whom there is neurospinal dysraphism. In such children there is necrosis of nervous tissue into the amniotic cavity and it would seem likely that this material has been antigenic to the lung in utero.", "contents": "Lymphoreticular aggregates in the newborn lung and their relation to intrauterine INFECTION and neurospinal dysraphism. A survey has been carried out of lymphoreticular aggregates in the lungs of 200 children, stillborns and dying within 15 days of birth and in a further 21 children with anencephalus, and controls. Lymphoreticular aggregates occur in response to amniotic infection and are found in the 'intrauterine infection syndrome'. They are not found in children showing early hyaline membranes. The time interval required for the production of lymphoreticular aggregates in the human child appears to be at lease 48 h and is probably 72 h before the production of well-formed aggregates and this probably applies to both the intrauterine and the postnatal state. There is a significant increase in the number of lungs showing lymphoreticular aggregates at birth in children in whom there is neurospinal dysraphism. In such children there is necrosis of nervous tissue into the amniotic cavity and it would seem likely that this material has been antigenic to the lung in utero."}
{"id": "PMID:1096972", "title": "Number fluctuation spectroscopy of motile microorganisms.", "content": "A random-walk model of motility is used to predict the dynamics of fluctuations in the number of particles in a small observation volume. The results show that number fluctuations provide a measure of the mean swimming speed as well as the persistence length. Experimental light-scattering results are presented for three strains of Escherichia coli whose motion appears random-walk in nature. For the strain with th elongest persistence length, excellent agreement is found that theoretical predictions. For the more erratic strains, however, the shape of the measured scattered light intensity correlation functions indicates the presence of a contribution due to orientational fluctuations.", "contents": "Number fluctuation spectroscopy of motile microorganisms. A random-walk model of motility is used to predict the dynamics of fluctuations in the number of particles in a small observation volume. The results show that number fluctuations provide a measure of the mean swimming speed as well as the persistence length. Experimental light-scattering results are presented for three strains of Escherichia coli whose motion appears random-walk in nature. For the strain with th elongest persistence length, excellent agreement is found that theoretical predictions. For the more erratic strains, however, the shape of the measured scattered light intensity correlation functions indicates the presence of a contribution due to orientational fluctuations."}
{"id": "PMID:1096973", "title": "Quantitative assessment of thymus-dependent (t)-cell function in human peripheral blood.", "content": "A new micromethod for evaluating lymphocyte responses to phytohemagglutinin (PHA) has been developed and used for the survey of T-cell function in human peripheral blood. The results of lymphocyte responses to PHA stimulation in whole blood culture were expressed in terms of unit volume of blood (50 mul). This new method has eliminated the major uncontrollable factors of conventional methods, and appears to be more correlated with the cell-mediated immunity in vivo.", "contents": "Quantitative assessment of thymus-dependent (t)-cell function in human peripheral blood. A new micromethod for evaluating lymphocyte responses to phytohemagglutinin (PHA) has been developed and used for the survey of T-cell function in human peripheral blood. The results of lymphocyte responses to PHA stimulation in whole blood culture were expressed in terms of unit volume of blood (50 mul). This new method has eliminated the major uncontrollable factors of conventional methods, and appears to be more correlated with the cell-mediated immunity in vivo."}
{"id": "PMID:1096974", "title": "Some characteristics of human rosette-forming cells.", "content": "Some evidence for identification of human rosette-forming cells as thymus and thymus-dependent lymphocytes (T cells) is presented. Application of the rosette assay to several clinical conditions including primary immunodeficiency diseases indicates the usefulness of this assay in quantitating the number of T cells in peripheral blood.", "contents": "Some characteristics of human rosette-forming cells. Some evidence for identification of human rosette-forming cells as thymus and thymus-dependent lymphocytes (T cells) is presented. Application of the rosette assay to several clinical conditions including primary immunodeficiency diseases indicates the usefulness of this assay in quantitating the number of T cells in peripheral blood."}
{"id": "PMID:1096975", "title": "Selective IgA deficiency---some perspectives.", "content": "The clinical spectrum of IgA deficiency varies from good health to respiratory, gastrointestinal, autoimmune and malignant disorders. Evaluation of B lymphocytes with surface IgA receptors in this disease reveals cap formation, decreased to normal density of IgA receptors, and IgA secretion following pokeweed stimulation. Further study of antigen-induced B-lymphocyte phenomena is needed. The presence of decreased numbers of T-cell markers in some individuals with IgA deficiency suggests a T-\"helper\" cell defect.", "contents": "Selective IgA deficiency---some perspectives. The clinical spectrum of IgA deficiency varies from good health to respiratory, gastrointestinal, autoimmune and malignant disorders. Evaluation of B lymphocytes with surface IgA receptors in this disease reveals cap formation, decreased to normal density of IgA receptors, and IgA secretion following pokeweed stimulation. Further study of antigen-induced B-lymphocyte phenomena is needed. The presence of decreased numbers of T-cell markers in some individuals with IgA deficiency suggests a T-\"helper\" cell defect."}
{"id": "PMID:1096976", "title": "T-cell deficiency in diGeorge syndrome.", "content": "DiGeorge syndrome is broadly defined as absence or hypoplasia of the thymus due to dysmorphogenesis of the third and fourth pharyngeal pouches in early embryonic life. Various tests of thymic function have been evaluated in some 19 infants and young children with this syndrome. Deficiency of T lymphocytes has been shown to occur in the presence of as much as 10 to 20% of the normal amount of histologically normal thymic tissue in the partial DiGeorge syndrome.", "contents": "T-cell deficiency in diGeorge syndrome. DiGeorge syndrome is broadly defined as absence or hypoplasia of the thymus due to dysmorphogenesis of the third and fourth pharyngeal pouches in early embryonic life. Various tests of thymic function have been evaluated in some 19 infants and young children with this syndrome. Deficiency of T lymphocytes has been shown to occur in the presence of as much as 10 to 20% of the normal amount of histologically normal thymic tissue in the partial DiGeorge syndrome."}
{"id": "PMID:1096977", "title": "Immunodeficiency and autoimmunity.", "content": "The following aspects of autoimmunity and immunodeficiency will be discussed: 1) Autoantibodies and autoimmunity and their relationships to human disease and particularly to the rheumatic diseases. 2) Relationships between human immunodeficiency diseases and autoimmunity including: a) autoimmune disease in patients with immunodeficiency; b) arthritis in patients with immunodeficiency; c) lupus-like illness in carriers of chronic granulomatous disease; d) occurrence of autoantibodies and autoimmunity in relatives of patients with immunodeficiency. 3) Immunodeficiency as a basis for human rheumatic or autoimmune diseases. The role of immunodeficiency in NZB mouse diseases and human SLE. 4) The role of viral infections in autoimmune disease.", "contents": "Immunodeficiency and autoimmunity. The following aspects of autoimmunity and immunodeficiency will be discussed: 1) Autoantibodies and autoimmunity and their relationships to human disease and particularly to the rheumatic diseases. 2) Relationships between human immunodeficiency diseases and autoimmunity including: a) autoimmune disease in patients with immunodeficiency; b) arthritis in patients with immunodeficiency; c) lupus-like illness in carriers of chronic granulomatous disease; d) occurrence of autoantibodies and autoimmunity in relatives of patients with immunodeficiency. 3) Immunodeficiency as a basis for human rheumatic or autoimmune diseases. The role of immunodeficiency in NZB mouse diseases and human SLE. 4) The role of viral infections in autoimmune disease."}
{"id": "PMID:1096978", "title": "Immune deficiency, autoimmunity and aging.", "content": "Immune deficiency states in man and animals have been observed at all ages---in the very young, in adult life and the very old. At all ages there appears to be a correlation between immune deficiency and propensity to produce autoantibodies and malignancy. Cellular replacement in immunologically deficient mice results in correction of immune function and slightly increased survival while selected hybridization produces dramatic increases in survival and a delayed onset of autoimmune symptoms. Aging is defined in terms of primary and secondary events where a hypothetic rate of decline of function, called optimum functional longevity, is arbitrarily assigned to the long-lived population. Optimal functional longevity is genetically based and controlled by a hypothetic longevity homeostasis gene complex which includes Ir genes and genes that control endocrine balance.", "contents": "Immune deficiency, autoimmunity and aging. Immune deficiency states in man and animals have been observed at all ages---in the very young, in adult life and the very old. At all ages there appears to be a correlation between immune deficiency and propensity to produce autoantibodies and malignancy. Cellular replacement in immunologically deficient mice results in correction of immune function and slightly increased survival while selected hybridization produces dramatic increases in survival and a delayed onset of autoimmune symptoms. Aging is defined in terms of primary and secondary events where a hypothetic rate of decline of function, called optimum functional longevity, is arbitrarily assigned to the long-lived population. Optimal functional longevity is genetically based and controlled by a hypothetic longevity homeostasis gene complex which includes Ir genes and genes that control endocrine balance."}
{"id": "PMID:1096979", "title": "T Cells in immunodeficiencies as evaluated by an antihuman T-cell serum.", "content": "An antihuman T-cell has been prepared in rabbits by infecting lymphocytes from a patient with Bruton-type agammaglobulinemia and absorption with cultured B-lymphoblast cells. Cytotoxicity of the antiserum was assayed with peripheral blood lymphocytes in the presence of rabbit complement and its specificity for T vs B cells was verified. This anti-T-cell serum killed 66% (range 49-78) of normal blood lymphocytes. In patients with Bruton-type agammaglobulinemia the percentage of cells sensitive to the antiserum was above normal (77-89%), whereas it was decreased in some patients with lepromatous leprosy or under antilymphocyte globulin therapy.", "contents": "T Cells in immunodeficiencies as evaluated by an antihuman T-cell serum. An antihuman T-cell has been prepared in rabbits by infecting lymphocytes from a patient with Bruton-type agammaglobulinemia and absorption with cultured B-lymphoblast cells. Cytotoxicity of the antiserum was assayed with peripheral blood lymphocytes in the presence of rabbit complement and its specificity for T vs B cells was verified. This anti-T-cell serum killed 66% (range 49-78) of normal blood lymphocytes. In patients with Bruton-type agammaglobulinemia the percentage of cells sensitive to the antiserum was above normal (77-89%), whereas it was decreased in some patients with lepromatous leprosy or under antilymphocyte globulin therapy."}
{"id": "PMID:1096980", "title": "Subacute sclerosing panencephalitis (SSPE).", "content": "Subacute sclerosing panencephalitis (SSPE) is a degenerative disease of the central nervous system occurring in children and adolescents. The measles virus, or a virus closely associated, plays an important role in the disease. SSPE appears years after these children have had the measles but still a measles-like virus can be found in their brain and in other organs. A certain deficit, still to be defined of the cell-mediated immunity as a chronic stimulation of the antibody response against the measles virus is documented in SSPE pointing to a possible immunologic imbalance in these patients. Studying the possibility of the presence of immune complexes in SSPE, immunofluorescent studies of kidney biopsy in SSPE patients have been performed. Complement deposits in the glomerular basement membrane have been found in most patients. The possible role of immune complexes in SSPE is still to be defined.", "contents": "Subacute sclerosing panencephalitis (SSPE). Subacute sclerosing panencephalitis (SSPE) is a degenerative disease of the central nervous system occurring in children and adolescents. The measles virus, or a virus closely associated, plays an important role in the disease. SSPE appears years after these children have had the measles but still a measles-like virus can be found in their brain and in other organs. A certain deficit, still to be defined of the cell-mediated immunity as a chronic stimulation of the antibody response against the measles virus is documented in SSPE pointing to a possible immunologic imbalance in these patients. Studying the possibility of the presence of immune complexes in SSPE, immunofluorescent studies of kidney biopsy in SSPE patients have been performed. Complement deposits in the glomerular basement membrane have been found in most patients. The possible role of immune complexes in SSPE is still to be defined."}
{"id": "PMID:1096981", "title": "Immunodeficiency in leprosy.", "content": "It is known that the impairment of cell-mediated immunity (CM) exists in lepromatous leprosy patients. This was shown by 1) delayed skin reaction to various test antigens, 2) decreased transformation of lymphocytes by various antigens, 3) delayed rejection of skin allograft, 4) decreased migration inhibitory fa-tor (MIF) formation, and 5) histologic study of the lymph node. In addition to these previous works, we have studied T and B lymphocytes in peripheral blood of leprosy patients; T cells by the technic of rosette formation, and B cells by staining surface immunoglobulins. The result of our study shows decreased numbers of T cells and increased numbers of B cells in peripheral blood of lepromatous patients. A the same time, we found that allogenic leukocyte infusion treatment reversed T and B-cell abnormalities. We know that repeated infusions of allogenic leukocytes dramatically brought about improvement of the clinical status of the patients, resolution of skin lesions, subsidence of erythema nodosum leprosum reaction, clearance of bacteria from the skin and lymph nodes, and reconstitution of peripheral lymph nodes.", "contents": "Immunodeficiency in leprosy. It is known that the impairment of cell-mediated immunity (CM) exists in lepromatous leprosy patients. This was shown by 1) delayed skin reaction to various test antigens, 2) decreased transformation of lymphocytes by various antigens, 3) delayed rejection of skin allograft, 4) decreased migration inhibitory fa-tor (MIF) formation, and 5) histologic study of the lymph node. In addition to these previous works, we have studied T and B lymphocytes in peripheral blood of leprosy patients; T cells by the technic of rosette formation, and B cells by staining surface immunoglobulins. The result of our study shows decreased numbers of T cells and increased numbers of B cells in peripheral blood of lepromatous patients. A the same time, we found that allogenic leukocyte infusion treatment reversed T and B-cell abnormalities. We know that repeated infusions of allogenic leukocytes dramatically brought about improvement of the clinical status of the patients, resolution of skin lesions, subsidence of erythema nodosum leprosum reaction, clearance of bacteria from the skin and lymph nodes, and reconstitution of peripheral lymph nodes."}
{"id": "PMID:1096982", "title": "Ataxia-telangiectasia: some historic, clinical and pathologic observations.", "content": "Although an isolated clinical case report was published in 1926 and another in 1941, ataxia-telangiectasia (A-T) was not established as a distinct entity until 1957, when it was first delineated clinicopathologically. Susceptibility to sinopulmonary infection was identified as the main cause of death and as the third major component of the syndrome; its heredofamilial nature was documented, and it was designated \"ataxia-telangiectasia.\" In a later review of 101 published cases, lymphoreticular malignancy emerged as the second most frequent cause of death. Although the thymus was found to be absent in the first reported autopsy in 1957 and the serum IgA deficiency was first recorded in 1961, A-T was not established as an immunodeficiency disease until 1963. Thymic abnormality and dysgammaglobulinemia explain the 2 main causes of death, sinopulmonary and neoplastic, but the immunodeficiency is probably not the central defect. It does not appear to explain either of the 2 main clinical diagnostic keys, the ataxia and the telangiectasia, or any of the other seemingly unrealted multisystemic facets of this complex disorder. Some of our most provocative long-term clinical observations and recent pathologic findings in our series of 9 autopsies are discussed.", "contents": "Ataxia-telangiectasia: some historic, clinical and pathologic observations. Although an isolated clinical case report was published in 1926 and another in 1941, ataxia-telangiectasia (A-T) was not established as a distinct entity until 1957, when it was first delineated clinicopathologically. Susceptibility to sinopulmonary infection was identified as the main cause of death and as the third major component of the syndrome; its heredofamilial nature was documented, and it was designated \"ataxia-telangiectasia.\" In a later review of 101 published cases, lymphoreticular malignancy emerged as the second most frequent cause of death. Although the thymus was found to be absent in the first reported autopsy in 1957 and the serum IgA deficiency was first recorded in 1961, A-T was not established as an immunodeficiency disease until 1963. Thymic abnormality and dysgammaglobulinemia explain the 2 main causes of death, sinopulmonary and neoplastic, but the immunodeficiency is probably not the central defect. It does not appear to explain either of the 2 main clinical diagnostic keys, the ataxia and the telangiectasia, or any of the other seemingly unrealted multisystemic facets of this complex disorder. Some of our most provocative long-term clinical observations and recent pathologic findings in our series of 9 autopsies are discussed."}
{"id": "PMID:1096983", "title": "immunodeficiency in ataxia-telangiectasia.", "content": "Ataxia-telangiectasia is characterized by endocrine, neurologic, hematologic, hepatic, cutaneous and immunologic abnormalities. The immunologic deficiencies vary considerably from patient to patient, and in each patient with respect to time. The most frequent deficiencies of humoral immunity are diminished or absent serum and salivary IgA, diminished or absent serum IgE and impaired antibody responses to a variety of bacterial and viral antigens. Deficiencies of cellular immunity are commonly found by both in vivo and in vitro analyses. Histologic confirmation of these immunodeficiencies is readily observed in the lymphoid tissue. The thymus, which may be the seat of the primary abnormality in the immunodeficiency syndrome, regularly shows morphologic characteristics of an embryonic thymus.", "contents": "immunodeficiency in ataxia-telangiectasia. Ataxia-telangiectasia is characterized by endocrine, neurologic, hematologic, hepatic, cutaneous and immunologic abnormalities. The immunologic deficiencies vary considerably from patient to patient, and in each patient with respect to time. The most frequent deficiencies of humoral immunity are diminished or absent serum and salivary IgA, diminished or absent serum IgE and impaired antibody responses to a variety of bacterial and viral antigens. Deficiencies of cellular immunity are commonly found by both in vivo and in vitro analyses. Histologic confirmation of these immunodeficiencies is readily observed in the lymphoid tissue. The thymus, which may be the seat of the primary abnormality in the immunodeficiency syndrome, regularly shows morphologic characteristics of an embryonic thymus."}
{"id": "PMID:1096984", "title": "A spectrum of B-cell differentiation defects.", "content": "The nonparallel relationships between development of classes of B lymphocytes and of serum immunoglobulins during ontogeny and in some patients imply: 1) that B-cell differentiation is discontinuous and 2) that class-specific-controls for terminal B-lymphocyte differentiation exist. Three separate defects in B-cell differentiation in vivo have been identified using pokeweed mitogen stimulation of lymphocytes. B lymphocytes may fail to develop, may develop but lack the capacity to differentiate further, or may be normal in both respects. The latter result suggests involvement of other cell types in antibody deficiency.", "contents": "A spectrum of B-cell differentiation defects. The nonparallel relationships between development of classes of B lymphocytes and of serum immunoglobulins during ontogeny and in some patients imply: 1) that B-cell differentiation is discontinuous and 2) that class-specific-controls for terminal B-lymphocyte differentiation exist. Three separate defects in B-cell differentiation in vivo have been identified using pokeweed mitogen stimulation of lymphocytes. B lymphocytes may fail to develop, may develop but lack the capacity to differentiate further, or may be normal in both respects. The latter result suggests involvement of other cell types in antibody deficiency."}
{"id": "PMID:1096985", "title": "Development of T and B cells and their functional interactions.", "content": "The development of IgM-, IgG- and then IgA-bearing B lymphocytes is an orderly process occurring independently of antigens and T cells. T cells enhance antigen-induced B lymphocyte to plasma cell differentiation according to the hierarchy: IgA greater than IgG greater than IgM. T cells also exhibit antigenindependent and dependent stages of differentiation and a functional diversity, perhaps attributable to different classes of T cells. New evidence indicates the presence of a receptor on T cells for IgM antibody produced by B cells.", "contents": "Development of T and B cells and their functional interactions. The development of IgM-, IgG- and then IgA-bearing B lymphocytes is an orderly process occurring independently of antigens and T cells. T cells enhance antigen-induced B lymphocyte to plasma cell differentiation according to the hierarchy: IgA greater than IgG greater than IgM. T cells also exhibit antigenindependent and dependent stages of differentiation and a functional diversity, perhaps attributable to different classes of T cells. New evidence indicates the presence of a receptor on T cells for IgM antibody produced by B cells."}
{"id": "PMID:1096986", "title": "Assessment of the B-lymphocyte population in agammaglobulinemia.", "content": "The population of B lymphocytes was assessed in the blood of 16 patients with agammaglobulinemia using immunofluorescence and EAC1423 reactivity as B-cell markers. Lymphocytes were fractionated on gradients of bovine serum albumin which are capable of separating lymphocytes into B-cell-rich and T-cell-rich populations. There was complete absence of B lymphocytes in the majority of patients with X-linked agammaglobulinemia. Normal or increased numbers of B lymphocytes were found in all patients with common variable agammaglobulinemia.", "contents": "Assessment of the B-lymphocyte population in agammaglobulinemia. The population of B lymphocytes was assessed in the blood of 16 patients with agammaglobulinemia using immunofluorescence and EAC1423 reactivity as B-cell markers. Lymphocytes were fractionated on gradients of bovine serum albumin which are capable of separating lymphocytes into B-cell-rich and T-cell-rich populations. There was complete absence of B lymphocytes in the majority of patients with X-linked agammaglobulinemia. Normal or increased numbers of B lymphocytes were found in all patients with common variable agammaglobulinemia."}
{"id": "PMID:1096987", "title": "Use of transfer factor in patients with depressed cellular immunity and chronic infection.", "content": "Two patients with chronic mucocutaneous candidiasis and a defect in cellular immunity received a single injection of dialysable transfer factor from Candida-positive donors in an effort to reconstitute immunologic function. The transfer of cellular hypersensitivity was successful in one of the two patients and was monitored by skin tests and MIF production; however, the effect was temporary and did not change the clinical course of the patient's infection. The other patient did not respond eitherimmunologically or clinically to transfer factor at this time, although she did respond subsequently to repeated doses of transfer factor and amphotericin B therapy (Pabst and Swanson: Brit. med. J. 2:442, 1972). In another instance transfer factor from tuberculin-positive donors was used successfully to eradicate an infection in a patient with progressive primary tuberculosis and an acquired defect in cellular immunity. The patient had not responded clinically or bacteriologically after 7 1/2 months of antituberculous therapy, although the organism was shown to be sensitive in vitro to the drugs she was receiving. She received 6 doses of dialysable transfer factor over a 3-month period and during this time she responded clinically, bacteriologically and roentgenographically.", "contents": "Use of transfer factor in patients with depressed cellular immunity and chronic infection. Two patients with chronic mucocutaneous candidiasis and a defect in cellular immunity received a single injection of dialysable transfer factor from Candida-positive donors in an effort to reconstitute immunologic function. The transfer of cellular hypersensitivity was successful in one of the two patients and was monitored by skin tests and MIF production; however, the effect was temporary and did not change the clinical course of the patient's infection. The other patient did not respond eitherimmunologically or clinically to transfer factor at this time, although she did respond subsequently to repeated doses of transfer factor and amphotericin B therapy (Pabst and Swanson: Brit. med. J. 2:442, 1972). In another instance transfer factor from tuberculin-positive donors was used successfully to eradicate an infection in a patient with progressive primary tuberculosis and an acquired defect in cellular immunity. The patient had not responded clinically or bacteriologically after 7 1/2 months of antituberculous therapy, although the organism was shown to be sensitive in vitro to the drugs she was receiving. She received 6 doses of dialysable transfer factor over a 3-month period and during this time she responded clinically, bacteriologically and roentgenographically."}
{"id": "PMID:1096988", "title": "Transfer factor as an approach to the treatment of immune deficiency disease.", "content": "Use of transfer factor in the treatment of chronic mucocutaneous candidiasis is discussed. The clinical experience in treating 2 patients with different clinical expressions of the syndrome and their different responses to treatment with repeated injections of transfer factor given in conjunction with amphotericin-B are reported. Results indicate that this form of therapy is a safe and effective way of restoring cell-mediated immunity to Candida and successfully treating some patients with chronic mucocutaneous candidiasis.", "contents": "Transfer factor as an approach to the treatment of immune deficiency disease. Use of transfer factor in the treatment of chronic mucocutaneous candidiasis is discussed. The clinical experience in treating 2 patients with different clinical expressions of the syndrome and their different responses to treatment with repeated injections of transfer factor given in conjunction with amphotericin-B are reported. Results indicate that this form of therapy is a safe and effective way of restoring cell-mediated immunity to Candida and successfully treating some patients with chronic mucocutaneous candidiasis."}
{"id": "PMID:1096989", "title": "Restoration of cell-mediated immune responses with transfer factor.", "content": "Five anergic patients with chronic mucocutaneous candidiasis were given transfer factor from donors with positive delayed reactions to Candida. In each recipient, the delayed skin reactions of the transfer factor donors appeared in the recipients and no recipient developed reactivities not possessed by the donor. Prior to injection of transfer factor, in vitro stimulation of the patients' lymphocytes with antigens did not result in MIF production, however, after transfer factor this response was positive. Therapy with transfer factor alone did not have therapeutic benefit, however, in 2 patients treatment with amphotericin-B followed by transfer factor has produced cutaneous remissions of 18 months.", "contents": "Restoration of cell-mediated immune responses with transfer factor. Five anergic patients with chronic mucocutaneous candidiasis were given transfer factor from donors with positive delayed reactions to Candida. In each recipient, the delayed skin reactions of the transfer factor donors appeared in the recipients and no recipient developed reactivities not possessed by the donor. Prior to injection of transfer factor, in vitro stimulation of the patients' lymphocytes with antigens did not result in MIF production, however, after transfer factor this response was positive. Therapy with transfer factor alone did not have therapeutic benefit, however, in 2 patients treatment with amphotericin-B followed by transfer factor has produced cutaneous remissions of 18 months."}
{"id": "PMID:1096990", "title": "Transfer factor II: results of therapy.", "content": "Transfer factor is a dialyzable extract of sensitized leukocytes, which transfers reactivity from skin test-positive donors to skin test-negative recipients. Transfer factor supplied by our laboratory has been used therapeutically to induce cellular immunity in 78 patients around the world. Many patients received multiple doses of transfer factor ranging from 1 unit given every 6 months for 3 years to 1 unit every week for 6 months to as much as 8 units per week for a brief period. A total of 299 units of transfer factor have been given. Diseases in which transfer factor appeared to cause improvement include the Wiskott-Aldrich syndrome, severe combined immunodeficiency disease, mucocutaneous candidiasis, chronic active hepatitis, coccidioidmycosis, dysgammaglobulinemia, Behcet disease, aphthous stomatitis, linear morphea, familial keratoacanthoma and malignancy.", "contents": "Transfer factor II: results of therapy. Transfer factor is a dialyzable extract of sensitized leukocytes, which transfers reactivity from skin test-positive donors to skin test-negative recipients. Transfer factor supplied by our laboratory has been used therapeutically to induce cellular immunity in 78 patients around the world. Many patients received multiple doses of transfer factor ranging from 1 unit given every 6 months for 3 years to 1 unit every week for 6 months to as much as 8 units per week for a brief period. A total of 299 units of transfer factor have been given. Diseases in which transfer factor appeared to cause improvement include the Wiskott-Aldrich syndrome, severe combined immunodeficiency disease, mucocutaneous candidiasis, chronic active hepatitis, coccidioidmycosis, dysgammaglobulinemia, Behcet disease, aphthous stomatitis, linear morphea, familial keratoacanthoma and malignancy."}
{"id": "PMID:1096993", "title": "Relation of lymphoid system and hormones to aging.", "content": "Congenital deficiencies of developmental hormones, such as growth hormone and thyroxine, are responsible for the thymus-dependent immunodeficiency disease observed in dwarf mice. Such an immunodeficiency state is associated with early aging phenomena. This fact suggests that lymphoid cells and primarily T-derived cells are involved in the control of aging processes.", "contents": "Relation of lymphoid system and hormones to aging. Congenital deficiencies of developmental hormones, such as growth hormone and thyroxine, are responsible for the thymus-dependent immunodeficiency disease observed in dwarf mice. Such an immunodeficiency state is associated with early aging phenomena. This fact suggests that lymphoid cells and primarily T-derived cells are involved in the control of aging processes."}
{"id": "PMID:1096994", "title": "Potentiating effect of fetal thymus on fetal liver cells for the promotion of recovery from the radiation injury in murine allogenic radiation chimeras or a little bit of thymus goes a long, long way.", "content": "Lethally irradiated CBA (H-2k) mice were treated with small numbers of liver cells from strain-A (H-2a) fetal donors. Recovery from the radiation injury was dependent upon the dose of liver cells administered. Giving very small numbers of fetal thymus cells, in addition to liver cells, had a highly significant and salutary effect on survival. The results suggest that clinical transplantation of liver cells from a single fetus might succeed if thymus cells are added.", "contents": "Potentiating effect of fetal thymus on fetal liver cells for the promotion of recovery from the radiation injury in murine allogenic radiation chimeras or a little bit of thymus goes a long, long way. Lethally irradiated CBA (H-2k) mice were treated with small numbers of liver cells from strain-A (H-2a) fetal donors. Recovery from the radiation injury was dependent upon the dose of liver cells administered. Giving very small numbers of fetal thymus cells, in addition to liver cells, had a highly significant and salutary effect on survival. The results suggest that clinical transplantation of liver cells from a single fetus might succeed if thymus cells are added."}
{"id": "PMID:1096995", "title": "Developmental immunity in the piglet.", "content": "Germfree colostrum-deprived piglets are immunologically \"virgin\" and extremely susceptible to microbial infection due to lack of passive maternal immunity. They are, however, highly immunologically competent as determined by their excellent immune response to various antigenic stimulation. The results suggest that antigen is the sole inducer of specific antibody formation and support the hypothesis that the initial step in the \"true\" primary immune response involves priming of multipotential uncommitted immunocompetent cells (\"virgin\" X cells) to committed monopotent cells (Y cells), and that Y cells proliferate and differentiate into antibody-forming cells (Z cells).", "contents": "Developmental immunity in the piglet. Germfree colostrum-deprived piglets are immunologically \"virgin\" and extremely susceptible to microbial infection due to lack of passive maternal immunity. They are, however, highly immunologically competent as determined by their excellent immune response to various antigenic stimulation. The results suggest that antigen is the sole inducer of specific antibody formation and support the hypothesis that the initial step in the \"true\" primary immune response involves priming of multipotential uncommitted immunocompetent cells (\"virgin\" X cells) to committed monopotent cells (Y cells), and that Y cells proliferate and differentiate into antibody-forming cells (Z cells)."}
{"id": "PMID:1096996", "title": "Congenital neutropenia: impaired maturation with diminished stem-cell input.", "content": "Children with congenital neutropenia (Kostman disease) present a syndrome of recurrent and eventually fatal bacterial infections. The disease is uniformly lethal and the mean age at death is 24 months. To attempt to better understand the nature of this inborn error, we have studied marrow and peripheral blood of 3 children with the disease. Along with classic morphologic analysis we have used the technic of soft agar bone marrow culture in vitro. All 3 patients showed marked deficiency of numbers of neutrophils in the peripheral blood, intermittent monocytosis especially with infection, and predominant nitroblue tetrazolium (NBT) dye staining in monocytes during infection. The marrow of all 3 patients showed evidence of maturation arrest at the promyelocyte stage and very few or no neutrophils. Two cases showed no neutrophils in the Rebuck skin window inflammatory study. The 3rd case, however, developed small numbers of neutrophils in the peripheral blood, up to 6%, but the first 2 patients, although studied on many occasions, did not show neutrophils above 1%. Evidence from soft agar culture of bone marrow in 1 patient was compatible with the view that development of committed neutrophil stem cells is defective. This patient did not develop neutrophils in soft agar culture and showed loose colonies developing only to promyelocytes. In the 2nd case, where depression of neutrophil development was somewhat less severe clinically, culture of the marrow in soft agar yielded normal neutrophil colonies and none of the loose colonies showing arrested maturation were seen. These findings suggest that the basic mechanism underlying the congenital neutropenias may differ from patient to patient.", "contents": "Congenital neutropenia: impaired maturation with diminished stem-cell input. Children with congenital neutropenia (Kostman disease) present a syndrome of recurrent and eventually fatal bacterial infections. The disease is uniformly lethal and the mean age at death is 24 months. To attempt to better understand the nature of this inborn error, we have studied marrow and peripheral blood of 3 children with the disease. Along with classic morphologic analysis we have used the technic of soft agar bone marrow culture in vitro. All 3 patients showed marked deficiency of numbers of neutrophils in the peripheral blood, intermittent monocytosis especially with infection, and predominant nitroblue tetrazolium (NBT) dye staining in monocytes during infection. The marrow of all 3 patients showed evidence of maturation arrest at the promyelocyte stage and very few or no neutrophils. Two cases showed no neutrophils in the Rebuck skin window inflammatory study. The 3rd case, however, developed small numbers of neutrophils in the peripheral blood, up to 6%, but the first 2 patients, although studied on many occasions, did not show neutrophils above 1%. Evidence from soft agar culture of bone marrow in 1 patient was compatible with the view that development of committed neutrophil stem cells is defective. This patient did not develop neutrophils in soft agar culture and showed loose colonies developing only to promyelocytes. In the 2nd case, where depression of neutrophil development was somewhat less severe clinically, culture of the marrow in soft agar yielded normal neutrophil colonies and none of the loose colonies showing arrested maturation were seen. These findings suggest that the basic mechanism underlying the congenital neutropenias may differ from patient to patient."}
{"id": "PMID:1096998", "title": "Human thymus-derived rosette-forming cells and immunologic diseases.", "content": "This paper describes some studies done on the human lymphocytes that bind to normal sheep red blood cells in a rosette formation. On the basis of human fetal studies and investigations of markers for B cells, it is demonstrated that rosette-forming cells are valid markers for T cells. These cells were looked at in various immunologic diseases. A modification of the basic technic, one that detects subpopulations of lymphocytes, was mainly used. Results correlate closely with the cellular immune status of the patient.", "contents": "Human thymus-derived rosette-forming cells and immunologic diseases. This paper describes some studies done on the human lymphocytes that bind to normal sheep red blood cells in a rosette formation. On the basis of human fetal studies and investigations of markers for B cells, it is demonstrated that rosette-forming cells are valid markers for T cells. These cells were looked at in various immunologic diseases. A modification of the basic technic, one that detects subpopulations of lymphocytes, was mainly used. Results correlate closely with the cellular immune status of the patient."}
{"id": "PMID:1096999", "title": "Immunoglobulin metabolism in disease.", "content": "Immunodeficiency and hypogammaglobulinemia are shown to occur by a variety of pathophysiologic mechanisms. Hypogammaglobulinemia may be caused by decreased synthesis of all classes of immunoglobulins, or may involve defective synthesis of selective classes of immunoglobulins. Alternatively, hypogammaglobulinemia may be due to a disorder of endogenous catabolic pathways of a single immunoglobulin class, or of all immunoglobulin classes. Finally, hypogammaglobulinemia may be caused by excessive loss of immunoglobulins into the urinary or gastrointestinal tracts.", "contents": "Immunoglobulin metabolism in disease. Immunodeficiency and hypogammaglobulinemia are shown to occur by a variety of pathophysiologic mechanisms. Hypogammaglobulinemia may be caused by decreased synthesis of all classes of immunoglobulins, or may involve defective synthesis of selective classes of immunoglobulins. Alternatively, hypogammaglobulinemia may be due to a disorder of endogenous catabolic pathways of a single immunoglobulin class, or of all immunoglobulin classes. Finally, hypogammaglobulinemia may be caused by excessive loss of immunoglobulins into the urinary or gastrointestinal tracts."}
{"id": "PMID:1097000", "title": "Platelet size in man.", "content": "The shape and parameters of platelet size distributions were studied in 50 normal persons and 97 patients in order to test the proposed thesis that platelet size heterogeneity results mainly from aging in the circulation. This thesis was contradicted (1) by size distributions of age-homogeneous, newly-born cell populations which were lognormal with increased (instead of decreased) dispersion of volumes and (2) by the macrothrombocytosis found in some populations with normal age distribution. For these reasons, thrombocytopoiesis appeared to play the major role in determining platelet size. A model was built in which the volume variation of platelet territories due to megakaryocyte growth and membrane demarcation at each step of maturation was a random proportion of the previous value of the volume. This model explains the lognormal shape of both newborn and circulating platelet size distributions. It  also implies that (1) the mean and standard deviation of platelet logvolumes depend on the rates of volume change of the individual platelet territories (growth rate minus demarcation rate) as well as on megakaryocyte maturation time; (2) platelet hyperdestruction causes an increase in the mean and dispersion of the rates of territory volume change; (3) Mediterranean macrothrombocytosis and some hereditary macrothrombocytotic thrombocytopenias or dysthrombocytopoieses reflect a diminished rate of territory demarcation, and (4) platelet size heterogeneity is caused mainly by the variations in territory growth and demarcation and not by aging in the circulation.", "contents": "Platelet size in man. The shape and parameters of platelet size distributions were studied in 50 normal persons and 97 patients in order to test the proposed thesis that platelet size heterogeneity results mainly from aging in the circulation. This thesis was contradicted (1) by size distributions of age-homogeneous, newly-born cell populations which were lognormal with increased (instead of decreased) dispersion of volumes and (2) by the macrothrombocytosis found in some populations with normal age distribution. For these reasons, thrombocytopoiesis appeared to play the major role in determining platelet size. A model was built in which the volume variation of platelet territories due to megakaryocyte growth and membrane demarcation at each step of maturation was a random proportion of the previous value of the volume. This model explains the lognormal shape of both newborn and circulating platelet size distributions. It  also implies that (1) the mean and standard deviation of platelet logvolumes depend on the rates of volume change of the individual platelet territories (growth rate minus demarcation rate) as well as on megakaryocyte maturation time; (2) platelet hyperdestruction causes an increase in the mean and dispersion of the rates of territory volume change; (3) Mediterranean macrothrombocytosis and some hereditary macrothrombocytotic thrombocytopenias or dysthrombocytopoieses reflect a diminished rate of territory demarcation, and (4) platelet size heterogeneity is caused mainly by the variations in territory growth and demarcation and not by aging in the circulation."}
{"id": "PMID:1097001", "title": "The development of vertebral bone marrow of human fetuses.", "content": "The development of the bone marrow of the thoracic vertebrae in seven human fetuses ranging from 95 to 150 mm in crown-rump length (CRL) was studied using light and electron microscopy. In the 95-mm CRL, hypertrophy of the chondrocytes occurred in the central region of the vertebrae, and blood vessels penetrated there from dorsal and ventral sides of the vertebral body. The primary marrow was represented by liberated cartilage lacunnae, occupied by the thin-walled blood vessels and a few mesenchymal cells and mononuclear cells containing granules or vacuoles (GMC). In the 99-mm CRL, chondroclasts were active in removing the cartilage near the central region of the vertebrae. Consequently, a large cavity was formed and occupied by a dilated sinus. GMC were numerous. Osteoblasts and osteocytes were increased in number. Reticular cells with long processes containing large amounts of glycogen began to appear in the extravascular space and formed the loosely arranged cellular meshwork of the hematopoietic compartment. Bundles of collagen fibrils were scattered in the meshwork. Hematopoietic cells were recognizable only in the 105-mm-CRL fetus and increased in number in the 120-mm-CRL fetus. The sinus endothelium was very thin and continuous without apertures except where blood cells crossed the wall. The developing blood cells lying against the outside of the sinus endothelium indented it. At points, collagen fibrils attached to the outside of endothelial cells and appeared to function as the anchoring filaments of lymphatics. The physiologic implications of the association of stromal cells, vascular sinuses, and hematopoietic cells are discussed in relationship to the microhematopoietic environment of the bone marrow.", "contents": "The development of vertebral bone marrow of human fetuses. The development of the bone marrow of the thoracic vertebrae in seven human fetuses ranging from 95 to 150 mm in crown-rump length (CRL) was studied using light and electron microscopy. In the 95-mm CRL, hypertrophy of the chondrocytes occurred in the central region of the vertebrae, and blood vessels penetrated there from dorsal and ventral sides of the vertebral body. The primary marrow was represented by liberated cartilage lacunnae, occupied by the thin-walled blood vessels and a few mesenchymal cells and mononuclear cells containing granules or vacuoles (GMC). In the 99-mm CRL, chondroclasts were active in removing the cartilage near the central region of the vertebrae. Consequently, a large cavity was formed and occupied by a dilated sinus. GMC were numerous. Osteoblasts and osteocytes were increased in number. Reticular cells with long processes containing large amounts of glycogen began to appear in the extravascular space and formed the loosely arranged cellular meshwork of the hematopoietic compartment. Bundles of collagen fibrils were scattered in the meshwork. Hematopoietic cells were recognizable only in the 105-mm-CRL fetus and increased in number in the 120-mm-CRL fetus. The sinus endothelium was very thin and continuous without apertures except where blood cells crossed the wall. The developing blood cells lying against the outside of the sinus endothelium indented it. At points, collagen fibrils attached to the outside of endothelial cells and appeared to function as the anchoring filaments of lymphatics. The physiologic implications of the association of stromal cells, vascular sinuses, and hematopoietic cells are discussed in relationship to the microhematopoietic environment of the bone marrow."}
{"id": "PMID:1097002", "title": "Regeneration of blood-forming organs after autologous leukocyte transfusion in lethally irradiated dogs. I. Distribution and cellularity of the bone marrow in normal dogs.", "content": "Marrow cellularity in adult beagles (1-2 yr old) is highest in centrally located bones, with values between 8000 and 12,000 nucleated cells per sq mm. It decreases gradually towards the peripheral parts of the body, reaching values below 1000 per sq mm in bones distal to the elbow and knee. The first tail segment always contains some active marrow. The fifth segment has only stromal elements. In spongy bones fat cells appear to be distributed at random among the blood-forming elements. In the middle part of the femur the fatty marrow predominates in the center of the cylinder, while the subendosteal area is very cellular. The proximal and distal ends of the femur are more cellular than the middle. The small standard deviation of the cellularity in the spongy bones of the trunk and in the proximal and middle part of the humerus makes these sites areas of choice for quantitative studies of marrow regeneration. The large variations in cellularity of the marrow in the radius and tibia of young adult dogs make these sites unsuitable for such studies. The distribution of active and fatty marrow in dogs is similar to that of humans. The differential count of active marrow is quite constant between different dogs and in the various sites of each animal.", "contents": "Regeneration of blood-forming organs after autologous leukocyte transfusion in lethally irradiated dogs. I. Distribution and cellularity of the bone marrow in normal dogs. Marrow cellularity in adult beagles (1-2 yr old) is highest in centrally located bones, with values between 8000 and 12,000 nucleated cells per sq mm. It decreases gradually towards the peripheral parts of the body, reaching values below 1000 per sq mm in bones distal to the elbow and knee. The first tail segment always contains some active marrow. The fifth segment has only stromal elements. In spongy bones fat cells appear to be distributed at random among the blood-forming elements. In the middle part of the femur the fatty marrow predominates in the center of the cylinder, while the subendosteal area is very cellular. The proximal and distal ends of the femur are more cellular than the middle. The small standard deviation of the cellularity in the spongy bones of the trunk and in the proximal and middle part of the humerus makes these sites areas of choice for quantitative studies of marrow regeneration. The large variations in cellularity of the marrow in the radius and tibia of young adult dogs make these sites unsuitable for such studies. The distribution of active and fatty marrow in dogs is similar to that of humans. The differential count of active marrow is quite constant between different dogs and in the various sites of each animal."}
{"id": "PMID:1097013", "title": "Myocardial failure with altered response to adrenaline in endotoxin shock.", "content": "1 There is a growing concensus that myocardial performance in the early stages of experimental endotoxic and septic shock is relatively normal; however, recent reports have identified an intermediate phase of shock when myocardial dysfunction is clearly apparent.2 The mechanism of dysfunction has become a subject of intense investigation. A current view is that altered myocardial responsiveness to circulating catecholamines may play an important role in the dysfunction observed after endotoxin administration. The present studies, in which an isolated working heart preparation of the dog was used, were designed to test this hypothesis. This particular experimental preparation was selected to provide an adequate interpretation of results; cardiac output, afterload, and concentrations of adrenaline reaching the coronary vascular bed were controlled in all experiments. Responses to infusions of adrenaline were recorded in the ;steady-state' condition. Control (non-shocked) heart responses to adrenaline were highly reproducible in terms of inotropic, chronotropic and coronary vascular behaviour.3 Results from the study document myocardial dysfunction within 4-6 h following an LD(70) endotoxin administration on the basis of increased left ventricular end diastolic pressure (LVEDP), decreased cardiac power and myocardial efficiency, and depressed negative and positive dP/dt parameters.4 Findings suggest significantly altered responsiveness of the myocardium to infused adrenaline at rates of 1, 2, and 5 mug/min with concentrations between 10 and 1 ng/ml blood. LVEDP was elevated while calculated power and efficiency parameters remained significantly below control values during infusion of adrenaline in endotoxin-treated hearts. Depressions of responsiveness were interpreted to occur on the basis of failure to restore positive and negative dP/dt to normal values and depressed coronary blood flow responses during adrenaline administration. Increases in coronary flow were regularly less in experimental hearts than the controls. Heart rate responses to adrenaline in both failing and non-failing hearts were identical.5 In conclusion, it is suggested that myocardial contractile and relaxation characteristics and coronary vascular responses to adrenaline infusion are depressed in endotoxin shock during the period of demonstrated myocardial dysfunction. No distinct causal relationships were observed between the altered myocardial responsiveness and pathogenesis of heart dysfunction since myocardial dysfunction and altered responsiveness to adrenaline were generally observed together. Myocardial oedema formation after endotoxin as previously reported by this laboratory may bear a relationship to the depressed negative dP/dt response to adrenaline.", "contents": "Myocardial failure with altered response to adrenaline in endotoxin shock. 1 There is a growing concensus that myocardial performance in the early stages of experimental endotoxic and septic shock is relatively normal; however, recent reports have identified an intermediate phase of shock when myocardial dysfunction is clearly apparent.2 The mechanism of dysfunction has become a subject of intense investigation. A current view is that altered myocardial responsiveness to circulating catecholamines may play an important role in the dysfunction observed after endotoxin administration. The present studies, in which an isolated working heart preparation of the dog was used, were designed to test this hypothesis. This particular experimental preparation was selected to provide an adequate interpretation of results; cardiac output, afterload, and concentrations of adrenaline reaching the coronary vascular bed were controlled in all experiments. Responses to infusions of adrenaline were recorded in the ;steady-state' condition. Control (non-shocked) heart responses to adrenaline were highly reproducible in terms of inotropic, chronotropic and coronary vascular behaviour.3 Results from the study document myocardial dysfunction within 4-6 h following an LD(70) endotoxin administration on the basis of increased left ventricular end diastolic pressure (LVEDP), decreased cardiac power and myocardial efficiency, and depressed negative and positive dP/dt parameters.4 Findings suggest significantly altered responsiveness of the myocardium to infused adrenaline at rates of 1, 2, and 5 mug/min with concentrations between 10 and 1 ng/ml blood. LVEDP was elevated while calculated power and efficiency parameters remained significantly below control values during infusion of adrenaline in endotoxin-treated hearts. Depressions of responsiveness were interpreted to occur on the basis of failure to restore positive and negative dP/dt to normal values and depressed coronary blood flow responses during adrenaline administration. Increases in coronary flow were regularly less in experimental hearts than the controls. Heart rate responses to adrenaline in both failing and non-failing hearts were identical.5 In conclusion, it is suggested that myocardial contractile and relaxation characteristics and coronary vascular responses to adrenaline infusion are depressed in endotoxin shock during the period of demonstrated myocardial dysfunction. No distinct causal relationships were observed between the altered myocardial responsiveness and pathogenesis of heart dysfunction since myocardial dysfunction and altered responsiveness to adrenaline were generally observed together. Myocardial oedema formation after endotoxin as previously reported by this laboratory may bear a relationship to the depressed negative dP/dt response to adrenaline."}
{"id": "PMID:1097010", "title": "[Validation of a new method for measurement of pulmonary blood volume (author's transl)].", "content": "The pulmonary blood volume (Q) can be measured by dye injection in the pulmonary artery (PA) and in the left atrium (LA) and by sampling in the brachial artery (double injection - single sampling method). We propose to replace the injection in the LA, for which a left heart catheterization is needed, by a pulmonary wedge injection (PW). No significant difference was found between the first and the second method in twenty-four measurements in six anesthetized and artificially ventilated dogs kept in steady hemodynamic state : QPW (1) equals 0.009 + 1.106 QLA (r equals 0.935). The results are independent of the capillary injection site. In man, the indirect validation was made by unilateral occlusion of a main pulmonary artery, allowing blood volume measurement of the occluded lung by two methods, one of them independent of the \"capillary\" injection. The results of both methods are comparable and highly correlated (r equals 0.86). The range of the values of pulmonary blood volume in sixteen normal supine subjects, at rest, was similar to that of other authors, whichever the technique they used (394 plus or minus 114 ml, 268 plus or minus 59 ml m-2). The reproducibility of the results was comparable. In five subjects, pulmonary blood volume did not change during a moderate muscular excerise in the supine position (57 watts). The pulmonary wedge injection in man can replace the LA injection of the double injection - single sampling method, without loss of precision nor effect on reproducibility.", "contents": "[Validation of a new method for measurement of pulmonary blood volume (author's transl)]. The pulmonary blood volume (Q) can be measured by dye injection in the pulmonary artery (PA) and in the left atrium (LA) and by sampling in the brachial artery (double injection - single sampling method). We propose to replace the injection in the LA, for which a left heart catheterization is needed, by a pulmonary wedge injection (PW). No significant difference was found between the first and the second method in twenty-four measurements in six anesthetized and artificially ventilated dogs kept in steady hemodynamic state : QPW (1) equals 0.009 + 1.106 QLA (r equals 0.935). The results are independent of the capillary injection site. In man, the indirect validation was made by unilateral occlusion of a main pulmonary artery, allowing blood volume measurement of the occluded lung by two methods, one of them independent of the \"capillary\" injection. The results of both methods are comparable and highly correlated (r equals 0.86). The range of the values of pulmonary blood volume in sixteen normal supine subjects, at rest, was similar to that of other authors, whichever the technique they used (394 plus or minus 114 ml, 268 plus or minus 59 ml m-2). The reproducibility of the results was comparable. In five subjects, pulmonary blood volume did not change during a moderate muscular excerise in the supine position (57 watts). The pulmonary wedge injection in man can replace the LA injection of the double injection - single sampling method, without loss of precision nor effect on reproducibility."}
{"id": "PMID:1097021", "title": "The natural history of renal and ureteric calculi.", "content": "In a prolonged follow-up of a series of 416 patients at The London Hospital Stone Clinic it was found that recurrence could still occur even as long as 10 years after the first stone, though this risk decrease slowly year by year. Recurrence is seldom related to hypercalciuria or urinary infection except when infection is caused by or associated with B. proteus in women. Claims for the value of any form of therapy for stone disease must be evaluated against the background of the natural history of lithiasis.", "contents": "The natural history of renal and ureteric calculi. In a prolonged follow-up of a series of 416 patients at The London Hospital Stone Clinic it was found that recurrence could still occur even as long as 10 years after the first stone, though this risk decrease slowly year by year. Recurrence is seldom related to hypercalciuria or urinary infection except when infection is caused by or associated with B. proteus in women. Claims for the value of any form of therapy for stone disease must be evaluated against the background of the natural history of lithiasis."}
{"id": "PMID:1097023", "title": "A clinical evaluation of hydrostatic pressure treatment for carcinoma of the bladder.", "content": "A small clinical series of patients treated by hydrostatic bladder dilatation is reported. The methods used, the complications and technical difficulties encountered are described. 4 patients out of 14, who had a complete course of treatment are free of any further tumour 12 months later, which in the context of their previous progress is a considerable improvement. These 4 patients did not appear to have any particular feature which, on clinical assessment, would have allowed us to be more selective in applying this treatment.", "contents": "A clinical evaluation of hydrostatic pressure treatment for carcinoma of the bladder. A small clinical series of patients treated by hydrostatic bladder dilatation is reported. The methods used, the complications and technical difficulties encountered are described. 4 patients out of 14, who had a complete course of treatment are free of any further tumour 12 months later, which in the context of their previous progress is a considerable improvement. These 4 patients did not appear to have any particular feature which, on clinical assessment, would have allowed us to be more selective in applying this treatment."}
{"id": "PMID:1097027", "title": "Orthotopic liver transplantation utilizing a vascular stapling instrument.", "content": "A technique of orthotopic liver transplantation in the pig is described in which the main feature is the accomplishment of four of the five anastomoses by the use of the Vogelfanger NRC vascular suturing instrument. The advantage of this instrument is the rapid accomplishment of safe leak-proof anastomoses.", "contents": "Orthotopic liver transplantation utilizing a vascular stapling instrument. A technique of orthotopic liver transplantation in the pig is described in which the main feature is the accomplishment of four of the five anastomoses by the use of the Vogelfanger NRC vascular suturing instrument. The advantage of this instrument is the rapid accomplishment of safe leak-proof anastomoses."}
{"id": "PMID:1097028", "title": "Renal transplantation in the unimmunosuppressed pig: an abnormal response.", "content": "A series of 82 kidney allografts and autografts in unimmunosuppressed pigs surviving for longer than 5 days has been compared with a previously reported series of 24 unimmunosuppressed pig kidney allografts. In the previous series 40 per cent survived for longer than 20 days. In the present series only 2 per cent of 55 transplants performed in cross-bred pigs obtained from different farms survived for longer than 20 days. The major causes of death were unrelated to rejection, prinicipally gastric ulcer bleeding. In the further 16 transplants between different pure-bred animals (thereby ensuring genetic disparity) the ulcer incidence was lowered by a gastro-enterostomy, and 12 per cent survived for longer than 20 days. Histological assessment of rejection in the latter group revealed 20 per cent with minimal rejection, was considered insufficient to account for the death of the animals. Based on the evaluation of 95 unimmunosuppressed pig kidney allografts in both series, it is concluded that a wide and unpredictable range of survival time occurs. Therefore the pig kidney allograft model is an unsatisfactory model in which to study immunosuppressive r\u00e9gimes. Also the unimmunosuppressed pig appears to show less evidence of kidney allograft rejection than the dog, irrespective of genetic disparity. It is tentatively suggested that this might be a lesser manifestation of the unexplained phenomenon of minimal rejection of liver allografts noted in unimmunosuppressed pigs.", "contents": "Renal transplantation in the unimmunosuppressed pig: an abnormal response. A series of 82 kidney allografts and autografts in unimmunosuppressed pigs surviving for longer than 5 days has been compared with a previously reported series of 24 unimmunosuppressed pig kidney allografts. In the previous series 40 per cent survived for longer than 20 days. In the present series only 2 per cent of 55 transplants performed in cross-bred pigs obtained from different farms survived for longer than 20 days. The major causes of death were unrelated to rejection, prinicipally gastric ulcer bleeding. In the further 16 transplants between different pure-bred animals (thereby ensuring genetic disparity) the ulcer incidence was lowered by a gastro-enterostomy, and 12 per cent survived for longer than 20 days. Histological assessment of rejection in the latter group revealed 20 per cent with minimal rejection, was considered insufficient to account for the death of the animals. Based on the evaluation of 95 unimmunosuppressed pig kidney allografts in both series, it is concluded that a wide and unpredictable range of survival time occurs. Therefore the pig kidney allograft model is an unsatisfactory model in which to study immunosuppressive r\u00e9gimes. Also the unimmunosuppressed pig appears to show less evidence of kidney allograft rejection than the dog, irrespective of genetic disparity. It is tentatively suggested that this might be a lesser manifestation of the unexplained phenomenon of minimal rejection of liver allografts noted in unimmunosuppressed pigs."}
{"id": "PMID:1097029", "title": "The use of the vas deferens in inguinal herniorrhaphy.", "content": "A new method of repair of inguinal herniae is described using the vas deferens as a suture material. It is especially suitable in very large and recurrent herniae. Nineteen cases have been operated on in this way with an average of 38 months' follow-up. In none has recurrence been noted. It is suggested that the vas deferens, so readily to hand, forms a more natural barrier than man-made fibres. It may also, in fact, remain biable though this had not, yet, been proved.", "contents": "The use of the vas deferens in inguinal herniorrhaphy. A new method of repair of inguinal herniae is described using the vas deferens as a suture material. It is especially suitable in very large and recurrent herniae. Nineteen cases have been operated on in this way with an average of 38 months' follow-up. In none has recurrence been noted. It is suggested that the vas deferens, so readily to hand, forms a more natural barrier than man-made fibres. It may also, in fact, remain biable though this had not, yet, been proved."}
{"id": "PMID:1097030", "title": "Screening methods for covert bacteriuria in schoolgirls.", "content": "Screening tests for bacteriuria based on two different principles were evaluated in1582 schoolgirls aged 5-11 years, and in 26 girls aged 3-16 years attending hospitalwith symptomatic urinary tract infection.  Tests for hypoglucosuria, performed by a semi-automated fluorometric method and with Uriglox strips on early-morning urine samples voided after overnight fasting, gave unacceptably high false-negative rates (16.7% and 20.8% respectively).  Oxoid and Uricult dipslides were immersed in fresh midstreamspecimens of urine obtained at school and read overnight incubation at 37 degrees C. Both gave comparable results, with low false-positive rates and no false-negative responses.  The higher cost of screening by dipslides was halved by using the \"dipstream\" technique, which also gave no false-negative results.  Its false-positive rate of 13.5% could be reduced to 1.8% by disregarding colony counts of 10-8 non-faecal organisms and over per litre, which appear unimportant in schoolchildren.  Bacteriuria was found in 2.3% of the schoolgirls; 39% of them had symptons, compared with 7.2% of the healthy girls, and 25% showed vesicoureteric reflux, which in 17% was associated with renalscarring.  Since the natural history of covert bacteriuria and its relationship withreflux and scarring remain undetermined further research is required.  The dipstreamtechnique offers a simple, reliable, and comparatively cheap screening method which could also be applied in general practice.", "contents": "Screening methods for covert bacteriuria in schoolgirls. Screening tests for bacteriuria based on two different principles were evaluated in1582 schoolgirls aged 5-11 years, and in 26 girls aged 3-16 years attending hospitalwith symptomatic urinary tract infection.  Tests for hypoglucosuria, performed by a semi-automated fluorometric method and with Uriglox strips on early-morning urine samples voided after overnight fasting, gave unacceptably high false-negative rates (16.7% and 20.8% respectively).  Oxoid and Uricult dipslides were immersed in fresh midstreamspecimens of urine obtained at school and read overnight incubation at 37 degrees C. Both gave comparable results, with low false-positive rates and no false-negative responses.  The higher cost of screening by dipslides was halved by using the \"dipstream\" technique, which also gave no false-negative results.  Its false-positive rate of 13.5% could be reduced to 1.8% by disregarding colony counts of 10-8 non-faecal organisms and over per litre, which appear unimportant in schoolchildren.  Bacteriuria was found in 2.3% of the schoolgirls; 39% of them had symptons, compared with 7.2% of the healthy girls, and 25% showed vesicoureteric reflux, which in 17% was associated with renalscarring.  Since the natural history of covert bacteriuria and its relationship withreflux and scarring remain undetermined further research is required.  The dipstreamtechnique offers a simple, reliable, and comparatively cheap screening method which could also be applied in general practice."}
{"id": "PMID:1097031", "title": "Prospective survey of availability of cadaveric kidneys for transplantation.", "content": "A prospective study in Nottingham hospitals, serving 3/4 million population, identified 25 potential kidney donors in one year.  Seventeen later died in circumstances when kidney donation would have been possible.  Twelve kidneys were actually removedfor transplantation out of the possible 34.  The reasons for failure to donate kidneyswere either relatives' refusal or a failure of the doctors looking after those patientsto consider them as potential donors.  If all possible donors had their kidneys removed there would be sufficient available to approximately equal the numbers of patients likely to require renal transplatation.", "contents": "Prospective survey of availability of cadaveric kidneys for transplantation. A prospective study in Nottingham hospitals, serving 3/4 million population, identified 25 potential kidney donors in one year.  Seventeen later died in circumstances when kidney donation would have been possible.  Twelve kidneys were actually removedfor transplantation out of the possible 34.  The reasons for failure to donate kidneyswere either relatives' refusal or a failure of the doctors looking after those patientsto consider them as potential donors.  If all possible donors had their kidneys removed there would be sufficient available to approximately equal the numbers of patients likely to require renal transplatation."}
{"id": "PMID:1097032", "title": "Chronic renal failure in Nottingham and requirements for dialysis and transplantfacilities.", "content": "A retrospective study of uraemic patients covering 12 months of 1970 and a prospective survey covering six months in 1973-4 is reported for a population of almost 3/4million.  The number of patients requiring regular dialysis treatment or transplantation or both is considered to be 45 per million of population under 65, 39 per million under 60, and 29 per million under 50 years of age.", "contents": "Chronic renal failure in Nottingham and requirements for dialysis and transplantfacilities. A retrospective study of uraemic patients covering 12 months of 1970 and a prospective survey covering six months in 1973-4 is reported for a population of almost 3/4million.  The number of patients requiring regular dialysis treatment or transplantation or both is considered to be 45 per million of population under 65, 39 per million under 60, and 29 per million under 50 years of age."}
{"id": "PMID:1097034", "title": "Attempts to programme ovulation with exogenous oestrogens and LH-RH analogue.", "content": "An attempt was made to programme ovulation in women on a predetermined day of the menstrual cycle by treatment used to induce ovulation in anovulatory sterility. At laparotomy for elective sterilization the ovaries were observed to assess the occurrence of ruptured follicles and ovulation. Histological analysis of ovaries and endometrium was performed, and ova were recovered from some women. Several regimens were tested but ovulation seldom occurred as planned. Clomiphene citrate, human chorionic gonadotrophin (HCG), synthetic luteinizing hormone releasing hormone (LH-RH), and ethinyloestradiol were ineffective. Human menopausal gonadotrophin followed by HCG was more effective, but multiple ovulations occurred. When one single injection of a potent long-acting LH-RH analogue was given on day 13 of the cycle to 10 women pretreated with ethinyloestradiol signs of recent ovulation were observed on day 15 in seven and on day 16 in two.", "contents": "Attempts to programme ovulation with exogenous oestrogens and LH-RH analogue. An attempt was made to programme ovulation in women on a predetermined day of the menstrual cycle by treatment used to induce ovulation in anovulatory sterility. At laparotomy for elective sterilization the ovaries were observed to assess the occurrence of ruptured follicles and ovulation. Histological analysis of ovaries and endometrium was performed, and ova were recovered from some women. Several regimens were tested but ovulation seldom occurred as planned. Clomiphene citrate, human chorionic gonadotrophin (HCG), synthetic luteinizing hormone releasing hormone (LH-RH), and ethinyloestradiol were ineffective. Human menopausal gonadotrophin followed by HCG was more effective, but multiple ovulations occurred. When one single injection of a potent long-acting LH-RH analogue was given on day 13 of the cycle to 10 women pretreated with ethinyloestradiol signs of recent ovulation were observed on day 15 in seven and on day 16 in two."}
{"id": "PMID:1097035", "title": "Histidine supplementation for treatment of anaemia of uraemia.", "content": "The effects of supplemental histidine on the anaemia of 16 chronically uraemic patients and 26 patients undergoing maintenance dialysis were assessed. Patients were given either L-histidine 4 g/day or placebo for a mean of 17-5 weeks in a randomized double-blind controlled study. Haemoglobin and packed cell volume increased slightly and to a similar degree in the patients on dialysis receiving placebo and histidine, but this change was statistically significant only in those on placebo. Plasma histidine rose significantly in the histidine-treated patients undergoing dialysis and decreased in the uraemic patients receiving placebo. These results suggest that supplementary L-histidine in patients with uraemia or patients undergoing maintenance dialysis does not improve anaemia.", "contents": "Histidine supplementation for treatment of anaemia of uraemia. The effects of supplemental histidine on the anaemia of 16 chronically uraemic patients and 26 patients undergoing maintenance dialysis were assessed. Patients were given either L-histidine 4 g/day or placebo for a mean of 17-5 weeks in a randomized double-blind controlled study. Haemoglobin and packed cell volume increased slightly and to a similar degree in the patients on dialysis receiving placebo and histidine, but this change was statistically significant only in those on placebo. Plasma histidine rose significantly in the histidine-treated patients undergoing dialysis and decreased in the uraemic patients receiving placebo. These results suggest that supplementary L-histidine in patients with uraemia or patients undergoing maintenance dialysis does not improve anaemia."}
{"id": "PMID:1097036", "title": "Effects of two types of dietary fibre on faecal steroid and lipid excretion.", "content": "Fibre supplements from wheat bran and sugar cane residue (bagasse) were added to the normal diet of volunteers for 12-week periods in a controlled metabolic study. Stool weights and stool fat excretion increased on both dietary fibres. Bagasse increased the daily loss of acid steroids, but bran failed to affect bile acid excretion. Decreased transit time without alteration in faecal flora occurred with bagasse. The raised excretion of bile acids and fatty acids failed to lower the plasma cholesterol and triglycerides after 12 weeks. Thus different fibre sources with variable components have dissimilar metabolic effects.", "contents": "Effects of two types of dietary fibre on faecal steroid and lipid excretion. Fibre supplements from wheat bran and sugar cane residue (bagasse) were added to the normal diet of volunteers for 12-week periods in a controlled metabolic study. Stool weights and stool fat excretion increased on both dietary fibres. Bagasse increased the daily loss of acid steroids, but bran failed to affect bile acid excretion. Decreased transit time without alteration in faecal flora occurred with bagasse. The raised excretion of bile acids and fatty acids failed to lower the plasma cholesterol and triglycerides after 12 weeks. Thus different fibre sources with variable components have dissimilar metabolic effects."}
{"id": "PMID:1097037", "title": "Increased frequency of neonatal jaundice in a maternity hospital.", "content": "The frequency of \"significant\" jaundice of the newborn at this hospital increased from 8-1% of all live births in 1971 to 12-1% in 1972 and 15-4% in 1973. This coincided with an increased use of oxytocic agents and epidural anaesthetics in labour, and a change in the artificial feed given to normal infants. A retrospective study of jaundiced infants born in 1972 failed to explain the increase in jaundice. Though the use of oxytocic agents was not the direct cause, since their use results in the delivery of more infants before 40 weeks of gestation it may be a contributory factor. The use of epidural anaesthetics was sastically related to the development of jaundice but the nature of the association was not clear. Mothers of infants who became jaundiced has a significantly higher frequency of poor past obstetric histories, but once again the association was not clear. The change in artificial feeds was excluded as a possible cause.", "contents": "Increased frequency of neonatal jaundice in a maternity hospital. The frequency of \"significant\" jaundice of the newborn at this hospital increased from 8-1% of all live births in 1971 to 12-1% in 1972 and 15-4% in 1973. This coincided with an increased use of oxytocic agents and epidural anaesthetics in labour, and a change in the artificial feed given to normal infants. A retrospective study of jaundiced infants born in 1972 failed to explain the increase in jaundice. Though the use of oxytocic agents was not the direct cause, since their use results in the delivery of more infants before 40 weeks of gestation it may be a contributory factor. The use of epidural anaesthetics was sastically related to the development of jaundice but the nature of the association was not clear. Mothers of infants who became jaundiced has a significantly higher frequency of poor past obstetric histories, but once again the association was not clear. The change in artificial feeds was excluded as a possible cause."}
{"id": "PMID:1097038", "title": "Emergence of group B streptococci in obstetric and perinatal infections.", "content": "A retrospective study of obstetric and perinatal illness due to group B streptococci during 1972-4 based on bacteriological referrals from Aberdeen Maternity Hospital and Special Nursery disclosed (1) a wide spectrum of maternal morbidity, particularly associated with amniotomy and a prolonged rupture-delivery interval, and (2) the emergence of the group B streptococcus as a major cause of serious neonatal infection in infants of low birth weight, often in the absence of maternal pyrexia. The group B isolates appeared to show a previously undocumented increased resistance to the aminoglycosides gentamicin and kanamycin. A prospective study of 369 random deliveries in Aberdeen Maternity Hospital showed a group B vaginal carriage rate of 49/1000; a neonatal colonization rate of 19/1000; maternal and neonatal morbidity rates of 16 and 2-7/1000, respectively; and an overall neonatal mortality of 1/1000 live births.", "contents": "Emergence of group B streptococci in obstetric and perinatal infections. A retrospective study of obstetric and perinatal illness due to group B streptococci during 1972-4 based on bacteriological referrals from Aberdeen Maternity Hospital and Special Nursery disclosed (1) a wide spectrum of maternal morbidity, particularly associated with amniotomy and a prolonged rupture-delivery interval, and (2) the emergence of the group B streptococcus as a major cause of serious neonatal infection in infants of low birth weight, often in the absence of maternal pyrexia. The group B isolates appeared to show a previously undocumented increased resistance to the aminoglycosides gentamicin and kanamycin. A prospective study of 369 random deliveries in Aberdeen Maternity Hospital showed a group B vaginal carriage rate of 49/1000; a neonatal colonization rate of 19/1000; maternal and neonatal morbidity rates of 16 and 2-7/1000, respectively; and an overall neonatal mortality of 1/1000 live births."}
{"id": "PMID:1097044", "title": "Prognosis for infants with idiopathic respiratory distress syndrome.", "content": "Infants with the idiopathic respiratory distress syndrome admitted to the intensive care unit during January 1972 to September 1974 were reviewed. The overall mortality rate for infants whose birth weight was 1000 g or more was under 10%, and for those who established spontaneous respiration after birth it was less than 5%. The hyperoxia test was not a useful guide to prognosis. It was possible on the basis of the infants' ability to establish spontaneous ventilation after birth to divide them into two groups. In those who established adequate ventilation the mortality rate was 4-5%; in those who did not it was 57%. This test should be generally applied, since not only does it give an immediate guide to the severity of the disease, which is better than that provided by birth weight, gestational age, or the hyperoxia test, but it may be applied to infants born in and outside a hospital providing neonatal intensive care. Improvement in the outlook for infants with a bad prognosis will be achieved only by improvements in perinatal care designed to minimize severe intrapartum asphyxia in infants of low birth weight.", "contents": "Prognosis for infants with idiopathic respiratory distress syndrome. Infants with the idiopathic respiratory distress syndrome admitted to the intensive care unit during January 1972 to September 1974 were reviewed. The overall mortality rate for infants whose birth weight was 1000 g or more was under 10%, and for those who established spontaneous respiration after birth it was less than 5%. The hyperoxia test was not a useful guide to prognosis. It was possible on the basis of the infants' ability to establish spontaneous ventilation after birth to divide them into two groups. In those who established adequate ventilation the mortality rate was 4-5%; in those who did not it was 57%. This test should be generally applied, since not only does it give an immediate guide to the severity of the disease, which is better than that provided by birth weight, gestational age, or the hyperoxia test, but it may be applied to infants born in and outside a hospital providing neonatal intensive care. Improvement in the outlook for infants with a bad prognosis will be achieved only by improvements in perinatal care designed to minimize severe intrapartum asphyxia in infants of low birth weight."}
{"id": "PMID:1097045", "title": "Evidence of gentamicin nephrotoxicity in patients with renal allografts.", "content": "Renal damage was assessed by measuring urinary enzyme excretion in 180 patients with renal allografts. Thirty-six of these patients were studied during 53 courses of treatment with antimicrobial agents which was the only antimicrobial agent which was associated with an increase in urinary enzyme activity. There was usually also evidence of reduced renal function. Renal morphological changes similar to those produced by gentamicin in rats were observed in human allograft biopsy specimens obtained during gentamicin treatment.", "contents": "Evidence of gentamicin nephrotoxicity in patients with renal allografts. Renal damage was assessed by measuring urinary enzyme excretion in 180 patients with renal allografts. Thirty-six of these patients were studied during 53 courses of treatment with antimicrobial agents which was the only antimicrobial agent which was associated with an increase in urinary enzyme activity. There was usually also evidence of reduced renal function. Renal morphological changes similar to those produced by gentamicin in rats were observed in human allograft biopsy specimens obtained during gentamicin treatment."}
{"id": "PMID:1097054", "title": "Cobalt iontophoresis techniques for tracing afferent and efferent connections in the vertebrate CNS.", "content": "In recent years several dye and cobalt iontophoresis techniques have been successfully used by invertebrate neurophysiologists for the localization of neuron somata and their processes. The cobalt iontophoresis technique has now been extended for use in the tracing of nerve fiber pathways and the localization of neuron somata in vertebrates. The brain and spinal cord of an animal are removed following perfusion with saline, and placed in a dish of cold saline. A suction electrode, filled with 300 mM cobalt chloride, is then placed over the cut end of the nerve trunk. Cobalt ions are then iontophoresed (by means of a voltage divider) within the nerve fibers, along their course. Following iontophoresis, the brain is bathed in an ammonium sulfide solution to precipitate the cobalt as black cobalt sulfide. The brain is then processed for histological procedures. A wide variety of vertebrates has been used, including amphibians, reptiles, aves and mammals, with uniform success. The cobalt iontophoresis technique presently in use has a wide range of applicability for neuroanatomical studies.", "contents": "Cobalt iontophoresis techniques for tracing afferent and efferent connections in the vertebrate CNS. In recent years several dye and cobalt iontophoresis techniques have been successfully used by invertebrate neurophysiologists for the localization of neuron somata and their processes. The cobalt iontophoresis technique has now been extended for use in the tracing of nerve fiber pathways and the localization of neuron somata in vertebrates. The brain and spinal cord of an animal are removed following perfusion with saline, and placed in a dish of cold saline. A suction electrode, filled with 300 mM cobalt chloride, is then placed over the cut end of the nerve trunk. Cobalt ions are then iontophoresed (by means of a voltage divider) within the nerve fibers, along their course. Following iontophoresis, the brain is bathed in an ammonium sulfide solution to precipitate the cobalt as black cobalt sulfide. The brain is then processed for histological procedures. A wide variety of vertebrates has been used, including amphibians, reptiles, aves and mammals, with uniform success. The cobalt iontophoresis technique presently in use has a wide range of applicability for neuroanatomical studies."}
{"id": "PMID:1097058", "title": "The role of intubation macro-dacryocystography in management of problems of the lacrimal system.", "content": "The Lloyd technique of dacryocystography has been described incorporating intubation, distension, macrographic and subtraction techniques. Excellent anatomic localization is possible. With this method, logical surgery may be planned.", "contents": "The role of intubation macro-dacryocystography in management of problems of the lacrimal system. The Lloyd technique of dacryocystography has been described incorporating intubation, distension, macrographic and subtraction techniques. Excellent anatomic localization is possible. With this method, logical surgery may be planned."}
{"id": "PMID:1097059", "title": "Role of lysosomal constituents of neutrophils in corneal graft reaction.", "content": "Band 2 protein exists in a firmly bound state in lysosomes, and is released when the cells concerned are specifically stimulated. The necessary stimulus can originate from different kinds of immunogenic interactions. The objective of this study was to determine whether during corneal graft reaction Band 2 protein could be detected in the host's cornea and aqueous humour. Band 2 protein was detected in the reacting host corneas (8 out of 23 eyes) and the aqueous humours (2 out of 23 eyes), mainly during the second and third postoperative weeks when parallel histological studies showed an accumulation of neutrophils in the graft bed. The severity of graft reaction appeared to run parallel to the amount of free Band 2 protein in the host cornea. After this period, in spite of the presence of a large number of neutrophils in the reacting host cornea, Band 2 protein was hardly detectable. This study indicates that the necessary stimulus for the secretion of Band 2 protein from rabbit neutrophils is present in the early stages of corneal xenograft reaction. The presence of the protein in the host cornea may be a factor in the development of the vascular response associated with the corneal graft reaction.", "contents": "Role of lysosomal constituents of neutrophils in corneal graft reaction. Band 2 protein exists in a firmly bound state in lysosomes, and is released when the cells concerned are specifically stimulated. The necessary stimulus can originate from different kinds of immunogenic interactions. The objective of this study was to determine whether during corneal graft reaction Band 2 protein could be detected in the host's cornea and aqueous humour. Band 2 protein was detected in the reacting host corneas (8 out of 23 eyes) and the aqueous humours (2 out of 23 eyes), mainly during the second and third postoperative weeks when parallel histological studies showed an accumulation of neutrophils in the graft bed. The severity of graft reaction appeared to run parallel to the amount of free Band 2 protein in the host cornea. After this period, in spite of the presence of a large number of neutrophils in the reacting host cornea, Band 2 protein was hardly detectable. This study indicates that the necessary stimulus for the secretion of Band 2 protein from rabbit neutrophils is present in the early stages of corneal xenograft reaction. The presence of the protein in the host cornea may be a factor in the development of the vascular response associated with the corneal graft reaction."}
{"id": "PMID:1097060", "title": "Migration of a wooden foreign body into the lens: report of a case.", "content": "A penetrating ocular injury caused by a tree branch resulted in a small corneoscleral laceration that healed after primary repair. Post-traumatic inflammation persisted and a vitreous mass developed. The mass finally protruded through the pupil and touched the posterior cornea. Histologic examination of the enucleated eye showed that the lens had been replaced by a wooden foreign body that filled the pupillary space and was surrounded by lens capsule. No intact lens fibers remained.", "contents": "Migration of a wooden foreign body into the lens: report of a case. A penetrating ocular injury caused by a tree branch resulted in a small corneoscleral laceration that healed after primary repair. Post-traumatic inflammation persisted and a vitreous mass developed. The mass finally protruded through the pupil and touched the posterior cornea. Histologic examination of the enucleated eye showed that the lens had been replaced by a wooden foreign body that filled the pupillary space and was surrounded by lens capsule. No intact lens fibers remained."}
{"id": "PMID:1097062", "title": "A somatomedin assay using normal rabbit cartilage in clinical studies.", "content": "Somatomedin (SM) is a growth hormone (GH) dependent biological growth factor in serum. A SM assay, using mechanically sliced discs from young normal rabbit costal cartilage was developed and evaluated. This assay is as sensitive and precise, while much less laborious and costly, than the hypophysectomized rat cartilage SM assay. The elevated serum SM levels in patients with acromegaly returned to normal in two patients who were cured, and remained elevated in four incompletely treated patients. The low serum SM levels in five GH deficient patients were increased to normal after treatment with human GH. SM levels were normal in 10 patients with normal GH levels and short stature due to various causes. SM levels in serum were very low in patients with chronic renal insufficiency; the mean SM level increased significantly after renal transplantation, however, it increased only minimally after hemodialysis. SM levels were subnormal in 14 patients with sever chronic liver disease. One patient with severe acute liver disease had low serum SM levels which increased after clinical recovery. These results further suggest that the liver produces SM, while the relationship of the kidney and SM is not clear.", "contents": "A somatomedin assay using normal rabbit cartilage in clinical studies. Somatomedin (SM) is a growth hormone (GH) dependent biological growth factor in serum. A SM assay, using mechanically sliced discs from young normal rabbit costal cartilage was developed and evaluated. This assay is as sensitive and precise, while much less laborious and costly, than the hypophysectomized rat cartilage SM assay. The elevated serum SM levels in patients with acromegaly returned to normal in two patients who were cured, and remained elevated in four incompletely treated patients. The low serum SM levels in five GH deficient patients were increased to normal after treatment with human GH. SM levels were normal in 10 patients with normal GH levels and short stature due to various causes. SM levels in serum were very low in patients with chronic renal insufficiency; the mean SM level increased significantly after renal transplantation, however, it increased only minimally after hemodialysis. SM levels were subnormal in 14 patients with sever chronic liver disease. One patient with severe acute liver disease had low serum SM levels which increased after clinical recovery. These results further suggest that the liver produces SM, while the relationship of the kidney and SM is not clear."}
{"id": "PMID:1097063", "title": "Effect of nitrogen source on carbon metabolism by Azotobacter vinelandii OP grown in chemostat and batch culture.", "content": "Azotobacter vinelandii growing under nitrogen-fixing conditions has higher specific activities of isocitric and malic dehydrogenase than do cells growing on nitrate or ammonia. Results show that the source of nitrogen has an effect on carbon metabolism.", "contents": "Effect of nitrogen source on carbon metabolism by Azotobacter vinelandii OP grown in chemostat and batch culture. Azotobacter vinelandii growing under nitrogen-fixing conditions has higher specific activities of isocitric and malic dehydrogenase than do cells growing on nitrate or ammonia. Results show that the source of nitrogen has an effect on carbon metabolism."}
{"id": "PMID:1097064", "title": "A new method for the isolation of methionyl transfer RNA synthetase mutants from Escherichia coli.", "content": "Six methionine auxotrophs were isolated from an E. coli K-12 strain which required up to 100 times as much methionine for growth as a conventional auxotroph. In these mutants the methionyl-tRNA synthetase had an increased Km for methionine. The Km value for the mutants ranged from 0.48 to 1.63 mM, compared to 0.078 mM for the wild type. The Km (methionine) for S-adenosyl methionine synthetase was not altered.", "contents": "A new method for the isolation of methionyl transfer RNA synthetase mutants from Escherichia coli. Six methionine auxotrophs were isolated from an E. coli K-12 strain which required up to 100 times as much methionine for growth as a conventional auxotroph. In these mutants the methionyl-tRNA synthetase had an increased Km for methionine. The Km value for the mutants ranged from 0.48 to 1.63 mM, compared to 0.078 mM for the wild type. The Km (methionine) for S-adenosyl methionine synthetase was not altered."}
{"id": "PMID:1097065", "title": "Immunofluorescence of peritoneal phagocytes after infection of mice with L-cell-attenuated Chlamydia psittaci 6BC.", "content": "Large amounts of particulate antigen of Chlamydia psittaci 6BC attenuated by growth in L cells were phagocytized by peritoneal mononuclear phagocytes during the 1st h after intraperitoneal inoculation. The phagocytes subsequently destroyed the immunofluorescent (IF) properties of the chlamydial antigens. It is suggested that the early damage of phagocytes by lysosomal enzymes activation induced by chlamydiae contributed to the relatively early disappearance of IF antigens from the peritoneal fluids.", "contents": "Immunofluorescence of peritoneal phagocytes after infection of mice with L-cell-attenuated Chlamydia psittaci 6BC. Large amounts of particulate antigen of Chlamydia psittaci 6BC attenuated by growth in L cells were phagocytized by peritoneal mononuclear phagocytes during the 1st h after intraperitoneal inoculation. The phagocytes subsequently destroyed the immunofluorescent (IF) properties of the chlamydial antigens. It is suggested that the early damage of phagocytes by lysosomal enzymes activation induced by chlamydiae contributed to the relatively early disappearance of IF antigens from the peritoneal fluids."}
{"id": "PMID:1097066", "title": "A new method of obtaining zygotes in Saccharomyces cerevisiae.", "content": "Experiments leading to a simple method of mass zygote formation in Saccharomyces cerevisiae are described. This method ordinarily requires only about 3 h of incubation, and consistently yielded 55-65% zygotes in seven different crosses among six different strains. Matings involving one strain required about 4 h of incubation but otherwise the results were comparable.", "contents": "A new method of obtaining zygotes in Saccharomyces cerevisiae. Experiments leading to a simple method of mass zygote formation in Saccharomyces cerevisiae are described. This method ordinarily requires only about 3 h of incubation, and consistently yielded 55-65% zygotes in seven different crosses among six different strains. Matings involving one strain required about 4 h of incubation but otherwise the results were comparable."}
{"id": "PMID:1097067", "title": "Nitrate utilization and growth in biotin-deficient Aspergillus nidulans.", "content": "A marked increase in the cellular synthesis accompanied by a decrease in the total fatty acid content was observed when Aspergillus nidulans was grown on NH4NO3 as a sole nitrogen source, in the medium containing avidin. Because of the increased uptake of NH4(plus), the level of NH4(plus), was lowered in the medium; as a result there is early uptake and assimilation of nitrate by a biotin-deficient culture as compared with the normal culture of A. nidulans. At about 17mM concentration, NH4(plus) repressed the activity of nitrate reductase. The regulation of NO3(minus) uptake and its assimilation with respect to the growth of A. nidulans have been discussed.", "contents": "Nitrate utilization and growth in biotin-deficient Aspergillus nidulans. A marked increase in the cellular synthesis accompanied by a decrease in the total fatty acid content was observed when Aspergillus nidulans was grown on NH4NO3 as a sole nitrogen source, in the medium containing avidin. Because of the increased uptake of NH4(plus), the level of NH4(plus), was lowered in the medium; as a result there is early uptake and assimilation of nitrate by a biotin-deficient culture as compared with the normal culture of A. nidulans. At about 17mM concentration, NH4(plus) repressed the activity of nitrate reductase. The regulation of NO3(minus) uptake and its assimilation with respect to the growth of A. nidulans have been discussed."}
{"id": "PMID:1097068", "title": "[Physical and chemical properties of tryptophenases of five species of Enterobacteriaceae].", "content": "The molecular weight, sedimentation coefficient, and amino acids composition were determined on five tryptophanases (TPases) from Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris and P. morganii. These TPases have identical sedimentation profile and coefficient (9.6 S), and the same molecular weight (220 000). Each enzyme is constituted of four identical subunits having a molecular weight of 55 000. The amino acids composition of these TPases is very similar, with the exception of P. morganii and P. vulgaris TPases which present significative variations in basic amino acids and tryptophan content. The species differentiation of the coli group cannot be made on their TPase characteristics only, contrary to P. morganii and P. vulgaris which can be differentiated between them and from the coli group.", "contents": "[Physical and chemical properties of tryptophenases of five species of Enterobacteriaceae]. The molecular weight, sedimentation coefficient, and amino acids composition were determined on five tryptophanases (TPases) from Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris and P. morganii. These TPases have identical sedimentation profile and coefficient (9.6 S), and the same molecular weight (220 000). Each enzyme is constituted of four identical subunits having a molecular weight of 55 000. The amino acids composition of these TPases is very similar, with the exception of P. morganii and P. vulgaris TPases which present significative variations in basic amino acids and tryptophan content. The species differentiation of the coli group cannot be made on their TPase characteristics only, contrary to P. morganii and P. vulgaris which can be differentiated between them and from the coli group."}
{"id": "PMID:1097069", "title": "[Differentiation of tryptophanases of five species of Enterobacteriaceae by sulfhydryl groups].", "content": "We have studied the sulfhydryl groups (-SH) on the tryptophanases (TPases) from Escherichia coli B. and E. aurescens, Shigella alkalescens, and Proteus vulgaris and P. morganii. The coli group and the P. morganii apo TPases have 20 -SH groups per mole of enzyme, where as P. vulgaris apoTPase has 16. In coli group TPases, there are 16 -SH groups on the mole surface and they are all implicated in the activity and the enzyme-substrate bond. Proteus morganii TPase has 8 surface -SH groups, 4 of which are implicated in the activity; the remaining 12 -SH groups are located inside the mole and take part in the activity and the enzyme-substrate bond. Proteus vulgaris TPase has 4 surface -SH groups which are constructive of the enzyme structure, whereas the 12 remaining -SH groups are located inside the mole and take part in the activity and the enzyme-substrate bond. It is concluded that Proteus TPases are molecules which have inverted quaternary structure in comparison to those of the coli group. The studied TPases have four subunits, each of them is constituted of one polypeptidic chain having a molecular weight of 55,000.", "contents": "[Differentiation of tryptophanases of five species of Enterobacteriaceae by sulfhydryl groups]. We have studied the sulfhydryl groups (-SH) on the tryptophanases (TPases) from Escherichia coli B. and E. aurescens, Shigella alkalescens, and Proteus vulgaris and P. morganii. The coli group and the P. morganii apo TPases have 20 -SH groups per mole of enzyme, where as P. vulgaris apoTPase has 16. In coli group TPases, there are 16 -SH groups on the mole surface and they are all implicated in the activity and the enzyme-substrate bond. Proteus morganii TPase has 8 surface -SH groups, 4 of which are implicated in the activity; the remaining 12 -SH groups are located inside the mole and take part in the activity and the enzyme-substrate bond. Proteus vulgaris TPase has 4 surface -SH groups which are constructive of the enzyme structure, whereas the 12 remaining -SH groups are located inside the mole and take part in the activity and the enzyme-substrate bond. It is concluded that Proteus TPases are molecules which have inverted quaternary structure in comparison to those of the coli group. The studied TPases have four subunits, each of them is constituted of one polypeptidic chain having a molecular weight of 55,000."}
{"id": "PMID:1097070", "title": "Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer.", "content": "Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive study has been undertaken utilizing this protocol (high hexose, complete medium under anaerobic growth transferred to low hexose, minimal medium with aerobic environment) to study the altered distribution of various metabolites during these transitions. Measurement of all the glycolytic, and some tricarboxylic acid intermediates during such a transition has elucidated the following points. On a qualitative basis, the metabolic controls prevalent in these growing cultures are similar to those identified in yeast under less physiological conditions. Also, the effect of anaerobic catabolism, which preconditions enzyme and pool levels in the yeast, is of paramount importance in determining the events subsequent to oxygen challenge. However, this experimental approach has severe limitations and does not lend itself to a clear-cut delineation of the events that occur during the process of mitochondriogenesis.", "contents": "Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer. Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive study has been undertaken utilizing this protocol (high hexose, complete medium under anaerobic growth transferred to low hexose, minimal medium with aerobic environment) to study the altered distribution of various metabolites during these transitions. Measurement of all the glycolytic, and some tricarboxylic acid intermediates during such a transition has elucidated the following points. On a qualitative basis, the metabolic controls prevalent in these growing cultures are similar to those identified in yeast under less physiological conditions. Also, the effect of anaerobic catabolism, which preconditions enzyme and pool levels in the yeast, is of paramount importance in determining the events subsequent to oxygen challenge. However, this experimental approach has severe limitations and does not lend itself to a clear-cut delineation of the events that occur during the process of mitochondriogenesis."}
{"id": "PMID:1097071", "title": "Aerobic adaptation in yeast. III. Changes in metabolic intermediates during anaerobic-aerobic transitions in exponentially growing cultures.", "content": "The changes in mechanism of control and in the mode of metabolism which yeast cells undergo when an anaerobic-aerobic transition is imposed on them has received much attention. One experimental approach to this type of investigation has been a step-down transition. The data from our experiments (cf. papers I and II of this series) has shown that this model is too complex to arrive at meaningful interpretation. We therefore repeated these measurements of glycolytic and citric acid cycle metabolites in cells growing on either glucose or galactose media and exposed to exponential N2 yields 02 transitions. This type of experiment is designed to examine directly the effect of oxygen challenge. Interpretation of this new data shows that the major metabolic control operating in the two hexose cultures are different and suggests that adenine nucleotide controls glucose cells whereas galactose cell metabolism is controlled by the NAD/NADH balance. This concept is discussed.", "contents": "Aerobic adaptation in yeast. III. Changes in metabolic intermediates during anaerobic-aerobic transitions in exponentially growing cultures. The changes in mechanism of control and in the mode of metabolism which yeast cells undergo when an anaerobic-aerobic transition is imposed on them has received much attention. One experimental approach to this type of investigation has been a step-down transition. The data from our experiments (cf. papers I and II of this series) has shown that this model is too complex to arrive at meaningful interpretation. We therefore repeated these measurements of glycolytic and citric acid cycle metabolites in cells growing on either glucose or galactose media and exposed to exponential N2 yields 02 transitions. This type of experiment is designed to examine directly the effect of oxygen challenge. Interpretation of this new data shows that the major metabolic control operating in the two hexose cultures are different and suggests that adenine nucleotide controls glucose cells whereas galactose cell metabolism is controlled by the NAD/NADH balance. This concept is discussed."}
{"id": "PMID:1097072", "title": "Impairment of reactivity to lepromin by mycobacterial antigens related to, or identical with, Mycobacterium leprae.", "content": "Three hundred and twenty young children were injected with Bacillus Calmette-Guerin (BCG) saline, or with one of the mycobacterial cytoplasmic antigens related with Mycobacterium leprae. At an appropriate time thereafter they were tested for dermal hypersensitivity to the antigens and for reactions to lepromin. Whereas all the antigens induced cell-mediated immunity, the incidence and intensity of late response to lepromin were significantly reduced in children preinjected with the cytoplasmic mycobacterial antigens, as contrasted with increased lepromin reactivity in the BCG group and with the findings in saline-injected children.", "contents": "Impairment of reactivity to lepromin by mycobacterial antigens related to, or identical with, Mycobacterium leprae. Three hundred and twenty young children were injected with Bacillus Calmette-Guerin (BCG) saline, or with one of the mycobacterial cytoplasmic antigens related with Mycobacterium leprae. At an appropriate time thereafter they were tested for dermal hypersensitivity to the antigens and for reactions to lepromin. Whereas all the antigens induced cell-mediated immunity, the incidence and intensity of late response to lepromin were significantly reduced in children preinjected with the cytoplasmic mycobacterial antigens, as contrasted with increased lepromin reactivity in the BCG group and with the findings in saline-injected children."}
{"id": "PMID:1097073", "title": "Preparation of a dane core (hepatitis B) antigen from human plasma.", "content": "Hepatitis B surface antigen (HBsAg) positive plasma, obtained from blood donors, was used to prepare an antigen (HBcAg) from the inner core of the Dane particle, the larger of the two spherical particles found in the blood in hepatitis B infection. The antigen was prepared by a series of centrifugation procedures on caesium chloride and sucrose gradients. Dane particle-rich fractions obtained from these gradients and concentrated by centrifugation were treated with Tween 80 to remove the outer coats from the particles. The remaining Dane particle cores were recovered by velocity sedimentation on a sucrose gradient. Preparations containing the Dane cores were used as antigens in the immune electron microscopy and complement fixation tests to demonstrate the specificity of HBcAg and lack of fross relationship with HBsAg. HBcAg will be used for the study of hepatitis B infections in man.", "contents": "Preparation of a dane core (hepatitis B) antigen from human plasma. Hepatitis B surface antigen (HBsAg) positive plasma, obtained from blood donors, was used to prepare an antigen (HBcAg) from the inner core of the Dane particle, the larger of the two spherical particles found in the blood in hepatitis B infection. The antigen was prepared by a series of centrifugation procedures on caesium chloride and sucrose gradients. Dane particle-rich fractions obtained from these gradients and concentrated by centrifugation were treated with Tween 80 to remove the outer coats from the particles. The remaining Dane particle cores were recovered by velocity sedimentation on a sucrose gradient. Preparations containing the Dane cores were used as antigens in the immune electron microscopy and complement fixation tests to demonstrate the specificity of HBcAg and lack of fross relationship with HBsAg. HBcAg will be used for the study of hepatitis B infections in man."}
{"id": "PMID:1097074", "title": "Observations on the distribution and ecology of Clostridium botulinum type E in Alaska.", "content": "Environmental samples collected along the coastline and from the interior of Alaska were examined for the presence of Clostridium botulinum. Clostridium botulinum type E was detected in soils from 5 of 12 beaches; in 7 of 115 non-coastal soil samples; in sediments from six of eight locales; in gills of salmon from two fishing areas; and in the feces of 1 of 44 colonic samples from marine mammals. The basic biochemical characteristics of the isolates were determined. Tube tests for demonstrating gelatin liquefaction proved insensitive with these strains, whereas a plate test detected gelatinase in all isolates. The presence of multiple nidi and the continual discharge of organic materials into the environment may contribute to the perpetuation of botulinum spores by which foods prepared form marine animals become contaminated. An emphasis should be placed upon the need for measures to reduce environmental contamination, to reduce contamination during food preparation, and to alert continually the population of the hazard wherever botulism is endemic.", "contents": "Observations on the distribution and ecology of Clostridium botulinum type E in Alaska. Environmental samples collected along the coastline and from the interior of Alaska were examined for the presence of Clostridium botulinum. Clostridium botulinum type E was detected in soils from 5 of 12 beaches; in 7 of 115 non-coastal soil samples; in sediments from six of eight locales; in gills of salmon from two fishing areas; and in the feces of 1 of 44 colonic samples from marine mammals. The basic biochemical characteristics of the isolates were determined. Tube tests for demonstrating gelatin liquefaction proved insensitive with these strains, whereas a plate test detected gelatinase in all isolates. The presence of multiple nidi and the continual discharge of organic materials into the environment may contribute to the perpetuation of botulinum spores by which foods prepared form marine animals become contaminated. An emphasis should be placed upon the need for measures to reduce environmental contamination, to reduce contamination during food preparation, and to alert continually the population of the hazard wherever botulism is endemic."}
{"id": "PMID:1097075", "title": "Multiple-variant design for the enrichment of photosynthetic bacterial populations.", "content": "A design for the simultaneous variation of acetate, sulfide, and sulfate concentrations in 11 related media is described.  Selectivity of these media by a direct enumeration technique in solid culture was compared with that for enrichment in liquid culture.  Variation of nutritional parameters resulted in the selection of Rhodospirillaceae at initial enrichment concentrations of 0.1 g or less of Na2S-9 H2O per litre and Chromatiaceae at 0.1 to 1.0 g of g of Na2S-9 H2O per litre.  The survival of sulfate-reducing and coliform bacteria indicated interdependency of photosynthetic populations with the former and  competition with the latter.  Photosynthetic bacteria selectively cultivated in liquid enrichment were tentatively identified as Rhodopseudomonas capsulata, Rhodopseudomonas speroides.  Chromatium warmingii, Chromatium okenii, Thiospirillum, and Rhabdomonas.", "contents": "Multiple-variant design for the enrichment of photosynthetic bacterial populations. A design for the simultaneous variation of acetate, sulfide, and sulfate concentrations in 11 related media is described.  Selectivity of these media by a direct enumeration technique in solid culture was compared with that for enrichment in liquid culture.  Variation of nutritional parameters resulted in the selection of Rhodospirillaceae at initial enrichment concentrations of 0.1 g or less of Na2S-9 H2O per litre and Chromatiaceae at 0.1 to 1.0 g of g of Na2S-9 H2O per litre.  The survival of sulfate-reducing and coliform bacteria indicated interdependency of photosynthetic populations with the former and  competition with the latter.  Photosynthetic bacteria selectively cultivated in liquid enrichment were tentatively identified as Rhodopseudomonas capsulata, Rhodopseudomonas speroides.  Chromatium warmingii, Chromatium okenii, Thiospirillum, and Rhabdomonas."}
{"id": "PMID:1097076", "title": "Comparison of several methods for the isolation of salmonellae from egg products.", "content": "Lactose broth and buffered peptone water proved to be equally effective as preenrichment media in the isolation of salmonellae from egg products.  With tetrathionate bile brilliant green broth, incubated at 43 degrees C as enrichment medium, more samples were found to be positive than with selenite cystine broth incubated at 37 degrees C or 43 degreesC and selenite brilliant green broth incubated at 37 degrees C.  As selective agar, brilliant green agar proved to be more reliable than bismuth sulfite agar.  These results indicate that the ISO method for the isolation of salmonellae from meat and meat products can be successfully used for the examination of egg products.", "contents": "Comparison of several methods for the isolation of salmonellae from egg products. Lactose broth and buffered peptone water proved to be equally effective as preenrichment media in the isolation of salmonellae from egg products.  With tetrathionate bile brilliant green broth, incubated at 43 degrees C as enrichment medium, more samples were found to be positive than with selenite cystine broth incubated at 37 degrees C or 43 degreesC and selenite brilliant green broth incubated at 37 degrees C.  As selective agar, brilliant green agar proved to be more reliable than bismuth sulfite agar.  These results indicate that the ISO method for the isolation of salmonellae from meat and meat products can be successfully used for the examination of egg products."}
{"id": "PMID:1097077", "title": "Chromosomal location of mutations affecting the regualtion of biotin synthesis in Escherichia coli.", "content": "The chromosomal locations of biotin regulatory mutations, birA, bioR, and dhbB, of Escherichia coli are determined by transduction using phage P1.  All mutant genes are mapped between bfe and supM.", "contents": "Chromosomal location of mutations affecting the regualtion of biotin synthesis in Escherichia coli. The chromosomal locations of biotin regulatory mutations, birA, bioR, and dhbB, of Escherichia coli are determined by transduction using phage P1.  All mutant genes are mapped between bfe and supM."}
{"id": "PMID:1097078", "title": "Cell surface protein of Salmonella typhimurium.", "content": "Lactoperoxidase-catalyzed radioiodination with Na125I was performed both on intact Salmonella typhimurium 1195 and on ghost membrane isolated from the same bacterial strain. Ghost membrane was also prepared from radioiodinated whole bacteria. The labelled proteins from both these ghost membrane preparations were compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate to identify the cell surface proteinmfrom the results obtained it was concluded that one major protein with an apparent molecular weight of 1200-1300 was exposed on the exterior surface of the ghost membrane.", "contents": "Cell surface protein of Salmonella typhimurium. Lactoperoxidase-catalyzed radioiodination with Na125I was performed both on intact Salmonella typhimurium 1195 and on ghost membrane isolated from the same bacterial strain. Ghost membrane was also prepared from radioiodinated whole bacteria. The labelled proteins from both these ghost membrane preparations were compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate to identify the cell surface proteinmfrom the results obtained it was concluded that one major protein with an apparent molecular weight of 1200-1300 was exposed on the exterior surface of the ghost membrane."}
{"id": "PMID:1097079", "title": "Kinetics of yeast growth:inhibition-threshold substrate concentrations.", "content": "Two Candida species and a Saccharomyces were grown on ethyl alcohol, acetic acid, ethyl acetate, and I-butanol, and their growth rates at a series of concentrations of each substrate are reported. Substrate concentrations higher than a characteristic threshold substrate concentration Stheta inhibited growth in accordance with a new relationship: mu = mumaxS/(k+S) - i(S - Stheta, where i is an inhibition constant and the other symbols have their usual meanings. Complete inhibition, that is, no growth, takes place when S = Si, the total inhibition concentration. Values of Stheta, Si, and i were calculated for the examples studied. Also discussed are the observed lag periods and also the lowered yields at high substrate concentrations.", "contents": "Kinetics of yeast growth:inhibition-threshold substrate concentrations. Two Candida species and a Saccharomyces were grown on ethyl alcohol, acetic acid, ethyl acetate, and I-butanol, and their growth rates at a series of concentrations of each substrate are reported. Substrate concentrations higher than a characteristic threshold substrate concentration Stheta inhibited growth in accordance with a new relationship: mu = mumaxS/(k+S) - i(S - Stheta, where i is an inhibition constant and the other symbols have their usual meanings. Complete inhibition, that is, no growth, takes place when S = Si, the total inhibition concentration. Values of Stheta, Si, and i were calculated for the examples studied. Also discussed are the observed lag periods and also the lowered yields at high substrate concentrations."}
{"id": "PMID:1097080", "title": "Semiology of Tremors.", "content": "Since the description by Galen in the 2nd Century, A.D., clinical neurology has acknowledged the existence of two types of tremor: that which occurs at rest and that occuring during the execution of movement. With the help of refined methods of analysis, E.M.G. and cinephotography, the authors have carried out a detailed clinical assessment in more than 400 patients. The basic criterion used to define a tremor was the classical definition of Dejerine: \"An involuntary, rhythmical and symmetrical movement about an axis of equilibrium.\" As a result of this study, the conclusion has been reached that there are two types of tremor: postural tremor and tremor of attitude. Both are present while the limb remains immobile, whether by wilful design or when at rest in a position of posture and subject only to the action of gravity. During voluntary movement, tremor is not present. Irregular, asymmetrical and non-rhythmic oscillations may appear however - as in so-called intention tremor, of cerebellar origin - but this abnormal movement can hardly be called a real tremor. It is merely a manifestation of ataxia. As a consequence of this study, it is suggested that further understanding of the basic mechanism of tremor can be reached by the investigation of the central neural structures which are involved in the physiology of posture and attitude.", "contents": "Semiology of Tremors. Since the description by Galen in the 2nd Century, A.D., clinical neurology has acknowledged the existence of two types of tremor: that which occurs at rest and that occuring during the execution of movement. With the help of refined methods of analysis, E.M.G. and cinephotography, the authors have carried out a detailed clinical assessment in more than 400 patients. The basic criterion used to define a tremor was the classical definition of Dejerine: \"An involuntary, rhythmical and symmetrical movement about an axis of equilibrium.\" As a result of this study, the conclusion has been reached that there are two types of tremor: postural tremor and tremor of attitude. Both are present while the limb remains immobile, whether by wilful design or when at rest in a position of posture and subject only to the action of gravity. During voluntary movement, tremor is not present. Irregular, asymmetrical and non-rhythmic oscillations may appear however - as in so-called intention tremor, of cerebellar origin - but this abnormal movement can hardly be called a real tremor. It is merely a manifestation of ataxia. As a consequence of this study, it is suggested that further understanding of the basic mechanism of tremor can be reached by the investigation of the central neural structures which are involved in the physiology of posture and attitude."}
{"id": "PMID:1097081", "title": "Post-traumatic pulmonary insufficiency following chest trauma: identification and prophylaxis.", "content": "Post-traumatic pulmonary insufficiency is a syndrome characterized by tachypnea, increasing respiratory effort, progressive pulmonary infiltrates and relentless progressive hypoxemia. Prophylaxis of this syndrome currently comprises adequate treatment and depends upon its early recognition by frequent monitoring of blood gases, chest radiograph, and determination of values of circulatory variables. Once the syndrome is recognized in its early stages, artificial ventilation, with the possible addition of positive end-expiratory pressure, meticulous fluid therapy properly monitored, and prevention of infection constitute the hallmark of treatment.", "contents": "Post-traumatic pulmonary insufficiency following chest trauma: identification and prophylaxis. Post-traumatic pulmonary insufficiency is a syndrome characterized by tachypnea, increasing respiratory effort, progressive pulmonary infiltrates and relentless progressive hypoxemia. Prophylaxis of this syndrome currently comprises adequate treatment and depends upon its early recognition by frequent monitoring of blood gases, chest radiograph, and determination of values of circulatory variables. Once the syndrome is recognized in its early stages, artificial ventilation, with the possible addition of positive end-expiratory pressure, meticulous fluid therapy properly monitored, and prevention of infection constitute the hallmark of treatment."}
{"id": "PMID:1097083", "title": "Complications of closure of loop colostomy.", "content": "Among 71 patients undergoing loop colostomy closure, one complication or more developed after operation in 35. Most of the complications were not serious, but there were two anastomotic leaks and two patients died. Some of the complications may be prevented by avoiding the use of drains and by packing the skin and subcutaneous tissues open with Vaseline gauze. There does not appear to be any advantage in using antibiotics in preoperative bowel preparation. A one-layer intraperitoneal colostomy closure can be done safely but is frequently associated with minor complications.", "contents": "Complications of closure of loop colostomy. Among 71 patients undergoing loop colostomy closure, one complication or more developed after operation in 35. Most of the complications were not serious, but there were two anastomotic leaks and two patients died. Some of the complications may be prevented by avoiding the use of drains and by packing the skin and subcutaneous tissues open with Vaseline gauze. There does not appear to be any advantage in using antibiotics in preoperative bowel preparation. A one-layer intraperitoneal colostomy closure can be done safely but is frequently associated with minor complications."}
{"id": "PMID:1097084", "title": "Bacterial peritonitis and the bursting strength of intestinal anastomoses.", "content": "In order to study the bursting strength of intestinal anastomoses, resection of a wedge of ileum and creation of an anastomosis were performed, and peritonitis was induced in five groups of rats. Peritonitis was induced by intraperitoneal injection of bacterial suspensions of Escherichia coli, Pseudomonas  aeruginosa, Staphylococcus aureus and Clostridium welchii, and the exteriorized ileal loop in each rat was regularly inspected for peritoneal exudation, adhesions, hyperemia, obstruction, leakage and bursting strength. All bowel anastomoses performed in the presence of peritonitis were weaker than those done in a sterile field (control group) and the type of organism influenced the strength of the anastomosis and the histopathologic changes; in this study the weakest anastomoses were associated with E. coli infection.", "contents": "Bacterial peritonitis and the bursting strength of intestinal anastomoses. In order to study the bursting strength of intestinal anastomoses, resection of a wedge of ileum and creation of an anastomosis were performed, and peritonitis was induced in five groups of rats. Peritonitis was induced by intraperitoneal injection of bacterial suspensions of Escherichia coli, Pseudomonas  aeruginosa, Staphylococcus aureus and Clostridium welchii, and the exteriorized ileal loop in each rat was regularly inspected for peritoneal exudation, adhesions, hyperemia, obstruction, leakage and bursting strength. All bowel anastomoses performed in the presence of peritonitis were weaker than those done in a sterile field (control group) and the type of organism influenced the strength of the anastomosis and the histopathologic changes; in this study the weakest anastomoses were associated with E. coli infection."}
{"id": "PMID:1097090", "title": "Computer tomography of the brain in Hamilton.", "content": "Computer tomography, a new noninvasive, rapid and easily tolerated technique of brain examination, has been evaluated by analysis of 1000 examinations.  It is much more sensitive than conventional radiographic techniques and can resolve soft-tissue structures that differ only slightly in density.  It also provides direct visualization of the ventricular system.  The range of clinical applications is wide; it is especially useful in differentiating intracerebral hemorrhage from infarction, and in demonstrating many brain tumours, particularly supratentorial, though enhancement with a water-soluble contrast medium injected intravenously is often necessary.", "contents": "Computer tomography of the brain in Hamilton. Computer tomography, a new noninvasive, rapid and easily tolerated technique of brain examination, has been evaluated by analysis of 1000 examinations.  It is much more sensitive than conventional radiographic techniques and can resolve soft-tissue structures that differ only slightly in density.  It also provides direct visualization of the ventricular system.  The range of clinical applications is wide; it is especially useful in differentiating intracerebral hemorrhage from infarction, and in demonstrating many brain tumours, particularly supratentorial, though enhancement with a water-soluble contrast medium injected intravenously is often necessary."}
{"id": "PMID:1097091", "title": "Determination of 5-fluorouracil (NSC-19893) plasma levels in rats and man by isotope dilution-mass fragmentography.", "content": "A highly sensitive and specific rapid assay for 5-fluorouracil (5-FU) in plasma samples at concentrations as low as 1 ng/ml was developed using mass fragmentography. Highly enriched C14-5-FU was empolyed as an internal standard. A two-compartmental open model for the disposition of free 5-FU in plasma is proposed. The beta-phase of 5-FU plasma elimination describes the pool size of anabolic metabolites, assuming this model is correct. Injections of 5-fu at 15, 40, and 90 mg/kg in rats resulted in an alpha-phase of elimination from plasma with a half-life of 10-18 minutes. The beta-phase could only be measured after the injection of 90 mg/kg of 5-FU and amounted to about 20 hours in two rats. Higher doses resulted in an unproportional increase in 5-FU plasma concentrations in the beta-phase, thus establishing nonlinear kinetics, presumably of the degradative metabolism. Plasma concentrations of 5-FU in man were measured after oral and intravenous administration of 15 mg/kg of 5-FU. Plasma elimination half-lives after intravenous doses were 12 minutes and dropped below measurable levels 2 hours after administration in three patients. After oral administration, peak plasma levels of 5-FU varied between 0.8 and 60 mug/ml of 5-FU in three patients after 10 minutes to 2 hours. Two of these patients showed rapid absorption and a measurable beta-phase of 5-FU plasma elimination possibly indicating a larger anabolic pool size of 5-FU-derived nucleosides and nucleotides when compared to patients receiving intravenous doses. This study may provide a pharmacokinetic rationale to monitor patients receiving 5-FU treatment.", "contents": "Determination of 5-fluorouracil (NSC-19893) plasma levels in rats and man by isotope dilution-mass fragmentography. A highly sensitive and specific rapid assay for 5-fluorouracil (5-FU) in plasma samples at concentrations as low as 1 ng/ml was developed using mass fragmentography. Highly enriched C14-5-FU was empolyed as an internal standard. A two-compartmental open model for the disposition of free 5-FU in plasma is proposed. The beta-phase of 5-FU plasma elimination describes the pool size of anabolic metabolites, assuming this model is correct. Injections of 5-fu at 15, 40, and 90 mg/kg in rats resulted in an alpha-phase of elimination from plasma with a half-life of 10-18 minutes. The beta-phase could only be measured after the injection of 90 mg/kg of 5-FU and amounted to about 20 hours in two rats. Higher doses resulted in an unproportional increase in 5-FU plasma concentrations in the beta-phase, thus establishing nonlinear kinetics, presumably of the degradative metabolism. Plasma concentrations of 5-FU in man were measured after oral and intravenous administration of 15 mg/kg of 5-FU. Plasma elimination half-lives after intravenous doses were 12 minutes and dropped below measurable levels 2 hours after administration in three patients. After oral administration, peak plasma levels of 5-FU varied between 0.8 and 60 mug/ml of 5-FU in three patients after 10 minutes to 2 hours. Two of these patients showed rapid absorption and a measurable beta-phase of 5-FU plasma elimination possibly indicating a larger anabolic pool size of 5-FU-derived nucleosides and nucleotides when compared to patients receiving intravenous doses. This study may provide a pharmacokinetic rationale to monitor patients receiving 5-FU treatment."}
{"id": "PMID:1097092", "title": "Clinical trials with 1-acetyl-2-picolinoylhydrazine (NSC-68626) in children.", "content": "Thirty-seven children with various malignant neoplasms were treated with 1-acetyl-2-picolinoylhydrazine. Complete regressions were documented in one child with embryonal rhabdomyosarcoma, in one child with generalized histiocytosis-X, and in one child with Hodgkin's disease. One patient had renal failure while receiving the drug but no conclusive cause and effect relationship could be established. Toxic effects otherwise were mild and consisted primarily of nausea and vomiting.", "contents": "Clinical trials with 1-acetyl-2-picolinoylhydrazine (NSC-68626) in children. Thirty-seven children with various malignant neoplasms were treated with 1-acetyl-2-picolinoylhydrazine. Complete regressions were documented in one child with embryonal rhabdomyosarcoma, in one child with generalized histiocytosis-X, and in one child with Hodgkin's disease. One patient had renal failure while receiving the drug but no conclusive cause and effect relationship could be established. Toxic effects otherwise were mild and consisted primarily of nausea and vomiting."}
{"id": "PMID:1097093", "title": "Adriamycin (NSC-123127) in the treatment of alkylator-resistant multiple myeloma: a pilot study.", "content": "Adriamycin was given in a dose of 25-45 mg/m2 of body surface area to nine patients with multiple myeloma who had failed to respond to intermittent therapy with melphalan and prednisone. Three patients had evidence of clinical remission and in two of them there was a 60% decrease in total body myeloma cell number. However, remissions were of short duration (3 months in two patients and over 10 months in another patient) and bone marrow suppression was moderately severe. Adriamycin may prove more useful in myeloma therapy when combined with melphalan or cyclophosphamide during the initial attempt at remission induction.", "contents": "Adriamycin (NSC-123127) in the treatment of alkylator-resistant multiple myeloma: a pilot study. Adriamycin was given in a dose of 25-45 mg/m2 of body surface area to nine patients with multiple myeloma who had failed to respond to intermittent therapy with melphalan and prednisone. Three patients had evidence of clinical remission and in two of them there was a 60% decrease in total body myeloma cell number. However, remissions were of short duration (3 months in two patients and over 10 months in another patient) and bone marrow suppression was moderately severe. Adriamycin may prove more useful in myeloma therapy when combined with melphalan or cyclophosphamide during the initial attempt at remission induction."}
{"id": "PMID:1097096", "title": "Phase I. evaluation of cyclocytidine (NSC-145668).", "content": "Cyclocytidine is a repository form of cytosine arabinoside and has a prolonged plasma half-life (8 hours) compared to cytosine arabinoside (12 minutes). A phase I trail was undertaken to establish the maximum tolerated dose. Nineteen patients were entered into the study (ten children, nine adults) and the dose which achieved consistent myelosuppression was established in five escalation increments as 600 mg/m for 10 days. This dose is consistent with previously reported data on the basic pharmacology of cyclocytidine and with optimum dose data in aminal tumor systems. Limiting toxicity, however, was related to the induction of postural hypotension which is not dose related. Recommendation to proceed with phase II studies of cyclocytidine must await clarification of the mechanism and prevention of the postural hypotension.", "contents": "Phase I. evaluation of cyclocytidine (NSC-145668). Cyclocytidine is a repository form of cytosine arabinoside and has a prolonged plasma half-life (8 hours) compared to cytosine arabinoside (12 minutes). A phase I trail was undertaken to establish the maximum tolerated dose. Nineteen patients were entered into the study (ten children, nine adults) and the dose which achieved consistent myelosuppression was established in five escalation increments as 600 mg/m for 10 days. This dose is consistent with previously reported data on the basic pharmacology of cyclocytidine and with optimum dose data in aminal tumor systems. Limiting toxicity, however, was related to the induction of postural hypotension which is not dose related. Recommendation to proceed with phase II studies of cyclocytidine must await clarification of the mechanism and prevention of the postural hypotension."}
{"id": "PMID:1097095", "title": "Acute monocytic leukemia in children and its response to vinblastine (NSC-49842).", "content": "Seventeen children with acute monocytic leukemia were treated with (a) vinblatine (with or without prednisone), 0.2 mg/kg of body weight twice a week (13 patients); (b) 6-mercaptopurine plus prednisone patient). Four patients died within the 1st week, before having an adequate chemotherapeutic trial, and a fifth patient died of spesis at 12 days. Five patients achieved complete remissions and two achieved in this study was 41%. For the 13 patients who survived more than 1 week, the remission rate was 53.8%. Remission occurred with vinblastine inall but one patient. Of the ten patients who recieved more than one dose of vinblastine, 60% achieved a remission, indicating that vinblastine is an active agent in this rare form of leukemia.", "contents": "Acute monocytic leukemia in children and its response to vinblastine (NSC-49842). Seventeen children with acute monocytic leukemia were treated with (a) vinblatine (with or without prednisone), 0.2 mg/kg of body weight twice a week (13 patients); (b) 6-mercaptopurine plus prednisone patient). Four patients died within the 1st week, before having an adequate chemotherapeutic trial, and a fifth patient died of spesis at 12 days. Five patients achieved complete remissions and two achieved in this study was 41%. For the 13 patients who survived more than 1 week, the remission rate was 53.8%. Remission occurred with vinblastine inall but one patient. Of the ten patients who recieved more than one dose of vinblastine, 60% achieved a remission, indicating that vinblastine is an active agent in this rare form of leukemia."}
{"id": "PMID:1097097", "title": "Evaluation of daunorubicin (NSC-82151) and methotrexate (NSC-740) in combination as a remission maintenance regimen in the treatment of acute leukemia.", "content": "The addition of a single monthy dose of daunorubicin (25 mg/m given intravenously) to the maximum tolerated dose of methotrexate (25 mg/m/dose given orally twice weekly) was evaluated as a maintenance regimen in 0 children with acute lymphoblastic leukemia. Seven patients who were nonevaluable were excluded from the study; 31 patients received methotrexate alone and 32 patients received a combination of methotrezate and daunorubicin. There was no significant difference between the distribution of remission times for the two maintenance  regimens (medians of 147 and 162 days, respectively). For patients with only one previous remission, the median length of remission in this study was significantly shorter than that of the original remission time. Despite the known myelosuppressive effects of both agents, daunorubicin did not cause additional toxicity nor did it interfere with the scheduled methotrexate therapy. Although the  addition of daunorubicin to methotrexate does not appear to be beneficial in the treatment of acute leukemia, this combination could be evaluated in other cancers in which both agents are known to be effective.", "contents": "Evaluation of daunorubicin (NSC-82151) and methotrexate (NSC-740) in combination as a remission maintenance regimen in the treatment of acute leukemia. The addition of a single monthy dose of daunorubicin (25 mg/m given intravenously) to the maximum tolerated dose of methotrexate (25 mg/m/dose given orally twice weekly) was evaluated as a maintenance regimen in 0 children with acute lymphoblastic leukemia. Seven patients who were nonevaluable were excluded from the study; 31 patients received methotrexate alone and 32 patients received a combination of methotrezate and daunorubicin. There was no significant difference between the distribution of remission times for the two maintenance  regimens (medians of 147 and 162 days, respectively). For patients with only one previous remission, the median length of remission in this study was significantly shorter than that of the original remission time. Despite the known myelosuppressive effects of both agents, daunorubicin did not cause additional toxicity nor did it interfere with the scheduled methotrexate therapy. Although the  addition of daunorubicin to methotrexate does not appear to be beneficial in the treatment of acute leukemia, this combination could be evaluated in other cancers in which both agents are known to be effective."}
{"id": "PMID:1097103", "title": "Factors influencing augmentation and/or acceleration of lymphoreticular tumors in mice by benzo(a)pyrene treatment.", "content": "The response of lymphoreticular tissues to a single i.p. injection of benzo(a)pyrene was studied in the first generation of C57BL/6J X C3HeB/FeJ F1 and C3HeB/FeJ X A/J F1 mice. Groups of 1-, 15-, and 42-day-old animals of both sexes received 75 or 150 mug of the carcinogen per g body weight. After a period of approximately 90 weeks, a high incidence (up to 43%) of reticulum cell sarcomas was observed in C57BL/6J X C3HeB/FeJ F1 mice treated with benzo(a)pyrene at 40 days of age. Animals treated with carcinogen at younger ages had a lower incidence of reticulum cell sarcomas. These sarcomas showed marked cellular pleomorphism and were classified into histiocytic, epitheloid-nodular, reticulocytic, and fibrocytic forms according to the predominant cell type. Lymphomas of thymic and extrathymic lymphoid origin and leukemia of granulocytic type were seen in a descending order of frequency. Control animals of either strain that were killed at 90 weeks of age were basically free of lymphoreticular tumors, while those kept under observation up to 170 weeks developed these tumors in 24% (C57BL X C3H F1) and 10% (C3H X A/J F1), respectively. Studies revealed that the augmentation and/or acceleration of development of the lymphoreticular neoplasms and specifically reticulum cell sarcomas by benzo(a)pyrene was dependent upon the strain and sex of mice used and the age at which the animals were exposed to carcinogen.", "contents": "Factors influencing augmentation and/or acceleration of lymphoreticular tumors in mice by benzo(a)pyrene treatment. The response of lymphoreticular tissues to a single i.p. injection of benzo(a)pyrene was studied in the first generation of C57BL/6J X C3HeB/FeJ F1 and C3HeB/FeJ X A/J F1 mice. Groups of 1-, 15-, and 42-day-old animals of both sexes received 75 or 150 mug of the carcinogen per g body weight. After a period of approximately 90 weeks, a high incidence (up to 43%) of reticulum cell sarcomas was observed in C57BL/6J X C3HeB/FeJ F1 mice treated with benzo(a)pyrene at 40 days of age. Animals treated with carcinogen at younger ages had a lower incidence of reticulum cell sarcomas. These sarcomas showed marked cellular pleomorphism and were classified into histiocytic, epitheloid-nodular, reticulocytic, and fibrocytic forms according to the predominant cell type. Lymphomas of thymic and extrathymic lymphoid origin and leukemia of granulocytic type were seen in a descending order of frequency. Control animals of either strain that were killed at 90 weeks of age were basically free of lymphoreticular tumors, while those kept under observation up to 170 weeks developed these tumors in 24% (C57BL X C3H F1) and 10% (C3H X A/J F1), respectively. Studies revealed that the augmentation and/or acceleration of development of the lymphoreticular neoplasms and specifically reticulum cell sarcomas by benzo(a)pyrene was dependent upon the strain and sex of mice used and the age at which the animals were exposed to carcinogen."}
{"id": "PMID:1097098", "title": "Hepatic arterial infusion for liver metastases from colon cancer: comparison of mitomycin C(NSC-26980) and 5-fluorouracil (NSC-19893).", "content": "This study compares the effectiveness of hepatic arterial infusion of mitomycin C with that of 5-fluorouracil in the management of carcinoma of the colon metastatic to the liver. A higher tumor response rate was obtained with 5-FU (ten of 12 patients) than with mitomycin C (six of 13 patients). The patients treated with mitomycin C had a median survival time of 9.5 months compared to 12 months for those receiving 5-FU, but this survival is not much different from that of the untreated historical controls. Femoral artery thrombosis requiring surgical thrombectomy developed in five of the 30 catheterized patients.", "contents": "Hepatic arterial infusion for liver metastases from colon cancer: comparison of mitomycin C(NSC-26980) and 5-fluorouracil (NSC-19893). This study compares the effectiveness of hepatic arterial infusion of mitomycin C with that of 5-fluorouracil in the management of carcinoma of the colon metastatic to the liver. A higher tumor response rate was obtained with 5-FU (ten of 12 patients) than with mitomycin C (six of 13 patients). The patients treated with mitomycin C had a median survival time of 9.5 months compared to 12 months for those receiving 5-FU, but this survival is not much different from that of the untreated historical controls. Femoral artery thrombosis requiring surgical thrombectomy developed in five of the 30 catheterized patients."}
{"id": "PMID:1097099", "title": "Adriamycin (NSC-123127) therapy for advanced gastrointestinal cancer.", "content": "Sixty-nine patients with advanced gastrointestinal carcinomas were given adriamycin intravenously at a dose level of 40-75 mg/m once every 3 weeks. Toxic effects included nausea, vomiting, diarrhea, stomatitis, alopecia, leukopenia, thrombocytopenia, and minor ECG changes. There was a slight trend toward move severe leukopenia in patients with markedly abnormal liver function test (serum glutamic oxaloacteic transaminase and alkaline phosphatase). Of the 57 pateints with colorectal cancer treated with adriamycin, four (7%) showed partial objective responses. In a controlled comparison of adriamycin versus 5-fluorouracil (5-FU) in patients with previously untreated large bowel carcinoma, three of 23 patients (13%) receiving adriamycin showed partial objective responses as compared with six of 25 patients (24%) receiving 5-FU. The median duration of response with adriamycin was 3 months com pared to over 6 months with 5-FU. Four of eight patients with gastric carcinoma showed partial objective responses. No responses were noted in a small number of patients with pancreatic and gallbladder carcinomas. Adriamycin would not seem to have any role in the treatment of advanced colorectal carcinoma. Our results, however, would justify further evaluation of this agent in gastric carcinoma.", "contents": "Adriamycin (NSC-123127) therapy for advanced gastrointestinal cancer. Sixty-nine patients with advanced gastrointestinal carcinomas were given adriamycin intravenously at a dose level of 40-75 mg/m once every 3 weeks. Toxic effects included nausea, vomiting, diarrhea, stomatitis, alopecia, leukopenia, thrombocytopenia, and minor ECG changes. There was a slight trend toward move severe leukopenia in patients with markedly abnormal liver function test (serum glutamic oxaloacteic transaminase and alkaline phosphatase). Of the 57 pateints with colorectal cancer treated with adriamycin, four (7%) showed partial objective responses. In a controlled comparison of adriamycin versus 5-fluorouracil (5-FU) in patients with previously untreated large bowel carcinoma, three of 23 patients (13%) receiving adriamycin showed partial objective responses as compared with six of 25 patients (24%) receiving 5-FU. The median duration of response with adriamycin was 3 months com pared to over 6 months with 5-FU. Four of eight patients with gastric carcinoma showed partial objective responses. No responses were noted in a small number of patients with pancreatic and gallbladder carcinomas. Adriamycin would not seem to have any role in the treatment of advanced colorectal carcinoma. Our results, however, would justify further evaluation of this agent in gastric carcinoma."}
{"id": "PMID:1097106", "title": "Adenocarcinoma of the pancreas in azaserine-treated rats.", "content": "Development of a model of carcinoma of the pancreas in rats was approached by attempting to identify chemicals that (a) behave as mutagens and (b) localize in the pancreas following systemic administration; and then to study the effects of long-term administration. Azaserine was selected because it behaves as a direct-acting mutagen in two bacterial test systems and because tissue distribution studies showed concentration especially in kidney and pancreas. Groups of rats have been given i.p. injections once or twice weekly for 6 months, and rats have been autopsied after 6 to 18 months. During the first year pancreases developed (a) nodules of atypical exocrine cells which seem to represent hyperplastic foci and (b) encapsulated adenomas. After 1 year most pancreases from treated rats are diffusely abnormal and contain many hyperplastic nodules and adenomas, while more than one-quarter have had pancreatic adenocarcimona. Metastases have been observed in lymph nodes, liver, and lung. No carcinomas or adenomas have been observed in control rats. No other organ shows as high an incidence of involvement as pancreas, but renal neoplasms were frequent. Studies with another chemical O-(N-methyl-N-nitroso-beta-alanyl)-L-serine, are at an earlier stage. The tissue distribution of radioactivity following injection of a 14C-labeled sample is similar to that of azaserine; however, this compound is not a direct-acting bacterial mutagen. Rats treated for 6 months twice weekly i.p. have a higher incidence of nodules of atypical acinar cells than did controls, although the number of nodules per rat is few. No adenomas or carcinomas have been found during 13 months of the study. We conclude that azaserine is a carcinogen in rats and causes major abnormalities of growth and differentiation of the exocrine pancreas, including adenocarcinoma in some rats. O-(N-Methyl-N-mitroso-beta-alanyl)-L-serine had less effect than azaserine on pancreatic growth and differentiation.", "contents": "Adenocarcinoma of the pancreas in azaserine-treated rats. Development of a model of carcinoma of the pancreas in rats was approached by attempting to identify chemicals that (a) behave as mutagens and (b) localize in the pancreas following systemic administration; and then to study the effects of long-term administration. Azaserine was selected because it behaves as a direct-acting mutagen in two bacterial test systems and because tissue distribution studies showed concentration especially in kidney and pancreas. Groups of rats have been given i.p. injections once or twice weekly for 6 months, and rats have been autopsied after 6 to 18 months. During the first year pancreases developed (a) nodules of atypical exocrine cells which seem to represent hyperplastic foci and (b) encapsulated adenomas. After 1 year most pancreases from treated rats are diffusely abnormal and contain many hyperplastic nodules and adenomas, while more than one-quarter have had pancreatic adenocarcimona. Metastases have been observed in lymph nodes, liver, and lung. No carcinomas or adenomas have been observed in control rats. No other organ shows as high an incidence of involvement as pancreas, but renal neoplasms were frequent. Studies with another chemical O-(N-methyl-N-nitroso-beta-alanyl)-L-serine, are at an earlier stage. The tissue distribution of radioactivity following injection of a 14C-labeled sample is similar to that of azaserine; however, this compound is not a direct-acting bacterial mutagen. Rats treated for 6 months twice weekly i.p. have a higher incidence of nodules of atypical acinar cells than did controls, although the number of nodules per rat is few. No adenomas or carcinomas have been found during 13 months of the study. We conclude that azaserine is a carcinogen in rats and causes major abnormalities of growth and differentiation of the exocrine pancreas, including adenocarcinoma in some rats. O-(N-Methyl-N-mitroso-beta-alanyl)-L-serine had less effect than azaserine on pancreatic growth and differentiation."}
{"id": "PMID:1097107", "title": "Factors affecting metabolism and mutagenicity of dimethylnitrosamine and diethylnitrosamine.", "content": "For exploration of the factors affecting dimethylnitrosamine (DMN) mutagenicity, for gathering of information on the metabolism of DMN, a frequently used and relatively well-understood carcinogen, and for explanation of metabolic variations in DMN carcinogenicity, parallel in vitro assays of the microsomal activation of  DMN to a mutagen and of DMN demethylation were performed. Salmonella typhimurium G46 reversions to histidine independence increase linearly with time of incubation for 30 min. At low concentrations of microsomal protein, increases in protein yield a more than proportional increase in mutations. Increasing DMN concentration saturates the enzyme, yielding less demethylation and fewer mutations proportionately. Mutagenesis is completely inhibited by 1 mM 2-diethyl-aminoethyl-2,2-diphenylvalerate. When both DMN and microsomal protein are varied at high concentrations, there is a simple linear relationship between mutagenicity and DMN demethylase activity. Thus DMN demethylase activity may be the primary controlling factor in the metabolism of DMN to a mutagen, and probably to a carcinogen; other simultaneous pathways of DMN metabolism proportional to demethylation have not been ruled out. Induction with both phenobarbital and 3-methylcholanthrene (3-MC) increased rat and mouse liver DMN demethylase activity. Mouse liver microsomes from the C57BL/6 strain demethylate DMN at a markedly lower rate than do microsomes from the C3H strain, but after 3-MC induction the relationship is reversed. Strain differences in activation of DMN were not found in the activation of diethylnitrosamine to a mutagen. Hepatic dealkylation of DMN and diethylnitrosamine to active mutagenic metabolites is increased in both rats and mice by both 3-MC and phenobarbital induction, which is in contrast to the findings of others that 3-MC and phenobarbital induction, which is in contrast to the findings of others that 3-MC decreases the incidence of DMN-induced hepatic tumors in rats, and phenobarbital decreases the incidence of diethylnitrosamine-induced hepatic tumors in mice.", "contents": "Factors affecting metabolism and mutagenicity of dimethylnitrosamine and diethylnitrosamine. For exploration of the factors affecting dimethylnitrosamine (DMN) mutagenicity, for gathering of information on the metabolism of DMN, a frequently used and relatively well-understood carcinogen, and for explanation of metabolic variations in DMN carcinogenicity, parallel in vitro assays of the microsomal activation of  DMN to a mutagen and of DMN demethylation were performed. Salmonella typhimurium G46 reversions to histidine independence increase linearly with time of incubation for 30 min. At low concentrations of microsomal protein, increases in protein yield a more than proportional increase in mutations. Increasing DMN concentration saturates the enzyme, yielding less demethylation and fewer mutations proportionately. Mutagenesis is completely inhibited by 1 mM 2-diethyl-aminoethyl-2,2-diphenylvalerate. When both DMN and microsomal protein are varied at high concentrations, there is a simple linear relationship between mutagenicity and DMN demethylase activity. Thus DMN demethylase activity may be the primary controlling factor in the metabolism of DMN to a mutagen, and probably to a carcinogen; other simultaneous pathways of DMN metabolism proportional to demethylation have not been ruled out. Induction with both phenobarbital and 3-methylcholanthrene (3-MC) increased rat and mouse liver DMN demethylase activity. Mouse liver microsomes from the C57BL/6 strain demethylate DMN at a markedly lower rate than do microsomes from the C3H strain, but after 3-MC induction the relationship is reversed. Strain differences in activation of DMN were not found in the activation of diethylnitrosamine to a mutagen. Hepatic dealkylation of DMN and diethylnitrosamine to active mutagenic metabolites is increased in both rats and mice by both 3-MC and phenobarbital induction, which is in contrast to the findings of others that 3-MC and phenobarbital induction, which is in contrast to the findings of others that 3-MC decreases the incidence of DMN-induced hepatic tumors in rats, and phenobarbital decreases the incidence of diethylnitrosamine-induced hepatic tumors in mice."}
{"id": "PMID:1097100", "title": "Intensive cyclophosphamide (NSC-26271) therapy for solid tumors.", "content": "Cyclophospamide was given in two dose schedules to 25 patients with a variety of nonlymphoid solid tumors. Eleven patients were given 18 courses of cyclophosphamide at a total dose of 60 mg/kg. Sixteen  patients received 26 courses at a total dose of 100 mg/kg. Two patients were treated with both regimens. Partial responses were achieved in two patients treated with 60-mg/kg dose of cyclophosphamide. One of these patients had osteogenic sarcoma and the other had renal carcinoma. The higher dose also produced two partial responses, one in a patient with anaplastic carcinom a of the lung and the other in a patient with anaplastic carcinoma of the lung and the other in a patient with embryonal testicular carcinoma. Mean leukocyte counts fell to a nadir of 1400 cells/mm after 60 mg/kg while they dropped to below 1000 cells/mm for 5 days after 100 mg/kg of cyclophosphamide. Mean platelet counts remained above 150,000 platelets/mm after both cyclophosphamide schedules. In fective complications were documented aftter three of the 18 courses at 60 mg/kg and after ten of the 26 courses at 100 mg5kg. In the latter group, there were three episodes of bacteremia, including one death from pseudomonas sepsis. Nonhematologic toxicity noted with the 100-mg/kg dose of cyclophosphamide included rare instances of electrocardiogram changes and serum enzyme alterations compatible with myocardial toxicity. The intensive cyclophosphamide therapy did not appear to result in an increased antitumor response in malignancies usually considered to be refractory to alkylating agents.", "contents": "Intensive cyclophosphamide (NSC-26271) therapy for solid tumors. Cyclophospamide was given in two dose schedules to 25 patients with a variety of nonlymphoid solid tumors. Eleven patients were given 18 courses of cyclophosphamide at a total dose of 60 mg/kg. Sixteen  patients received 26 courses at a total dose of 100 mg/kg. Two patients were treated with both regimens. Partial responses were achieved in two patients treated with 60-mg/kg dose of cyclophosphamide. One of these patients had osteogenic sarcoma and the other had renal carcinoma. The higher dose also produced two partial responses, one in a patient with anaplastic carcinom a of the lung and the other in a patient with anaplastic carcinoma of the lung and the other in a patient with embryonal testicular carcinoma. Mean leukocyte counts fell to a nadir of 1400 cells/mm after 60 mg/kg while they dropped to below 1000 cells/mm for 5 days after 100 mg/kg of cyclophosphamide. Mean platelet counts remained above 150,000 platelets/mm after both cyclophosphamide schedules. In fective complications were documented aftter three of the 18 courses at 60 mg/kg and after ten of the 26 courses at 100 mg5kg. In the latter group, there were three episodes of bacteremia, including one death from pseudomonas sepsis. Nonhematologic toxicity noted with the 100-mg/kg dose of cyclophosphamide included rare instances of electrocardiogram changes and serum enzyme alterations compatible with myocardial toxicity. The intensive cyclophosphamide therapy did not appear to result in an increased antitumor response in malignancies usually considered to be refractory to alkylating agents."}
{"id": "PMID:1097108", "title": "Metabolism of cigarette smoke condensates by human and rat homogenates to form mutagens detectable by Salmonella typhimurium TA1538.", "content": "Nineteen fractions of whole condensate of smoke from the University of Kentucky Reference Cigarette IRI were tested for mutagenicity in vitro using a bacterial indicator system. As little as 25 mug of the active fractions were mutagenic toward histidine-requiring Salmonella typhimurium TA1538, if the condensates were incubated in the presence of rat or human liver homogenates of lung were relatively inactive. Homogenates from livers of rats that had been treated with 3-methylcholanthrene converted condensates to mutagens more efficiently than did liver homogenates from man or from normal or phenobarbital-treated rats. Use of homogenates from animals treated with 3-methylcholanthrene gave much more reproducible results in smoke fraction assays because larger numbers of revertants were obtained, and dose-response curves were linear over the range 25 to 250 mug condensate. The linear dose-response curves permitted quantitative comparison of the various fractions. The mutagenicity per mg of basic fractions of whole smoke condensate is very high and that of neutral polycyclic hydrocarbons is very low. Because of the exquisite preferential sensitivity of the TA1538 test system to polycyclic amines and insensitively to alkyl polycyclics, there is a poor quantitative correlation between mutagenicity and carcinogenicity, as measured by skin painting or in vitro cell transformation. There is substantial evidence that many carcinogens are mutagens but that most of these compounds require metabolism before they are biologically active. If further development improves the sensitivity of the bacterial testing system to mutagenic derivatives of alkyl polycyclic and heteropolycyclic hydrocarbons, it may provide a convenient, rapid, quantitative, and inexpensive bioassay for the detection of potentially carcinogenic substances in tobacco smoke condensates.", "contents": "Metabolism of cigarette smoke condensates by human and rat homogenates to form mutagens detectable by Salmonella typhimurium TA1538. Nineteen fractions of whole condensate of smoke from the University of Kentucky Reference Cigarette IRI were tested for mutagenicity in vitro using a bacterial indicator system. As little as 25 mug of the active fractions were mutagenic toward histidine-requiring Salmonella typhimurium TA1538, if the condensates were incubated in the presence of rat or human liver homogenates of lung were relatively inactive. Homogenates from livers of rats that had been treated with 3-methylcholanthrene converted condensates to mutagens more efficiently than did liver homogenates from man or from normal or phenobarbital-treated rats. Use of homogenates from animals treated with 3-methylcholanthrene gave much more reproducible results in smoke fraction assays because larger numbers of revertants were obtained, and dose-response curves were linear over the range 25 to 250 mug condensate. The linear dose-response curves permitted quantitative comparison of the various fractions. The mutagenicity per mg of basic fractions of whole smoke condensate is very high and that of neutral polycyclic hydrocarbons is very low. Because of the exquisite preferential sensitivity of the TA1538 test system to polycyclic amines and insensitively to alkyl polycyclics, there is a poor quantitative correlation between mutagenicity and carcinogenicity, as measured by skin painting or in vitro cell transformation. There is substantial evidence that many carcinogens are mutagens but that most of these compounds require metabolism before they are biologically active. If further development improves the sensitivity of the bacterial testing system to mutagenic derivatives of alkyl polycyclic and heteropolycyclic hydrocarbons, it may provide a convenient, rapid, quantitative, and inexpensive bioassay for the detection of potentially carcinogenic substances in tobacco smoke condensates."}
{"id": "PMID:1097109", "title": "Quantitative immunofluorescence studies of the tumor antigen-bearing cell in giant cell tumor of bone and osteogenic sarcoma.", "content": "Tumor-associated antigen was found by reacting sera from two patients with giant cell tumor of bone with cells derived from their tumors, using autologous serum as intermediate reactant and fluorescein-conjugated goat anti-human IgG as final reactant. Approximately 40% of the plump, spindle-shaped cells that formed the background stroma of these tumors possessed the antigen; however, it was not present on giant cells. Fluorescence was much greater than that on similarly stained cells from 4 osteogenic sarcomas, suggesting that the antigenic density on cells from giant cell tumor was greater than that on cells from osteogenic sarcoma. Antibodies in sera from giant cell tumor patients and osteogenic sarcoma patients showed specific cross-reactivity. Stromal cells of giant cell tumors were established in culture and retained tumor-associated antigen, whereas giant cells failed to divide and detached from the flask within two weeks. Intensity of fluorescence (antigenic density) decreased with progressive passage levels, but a larger percentage of cells showed fluorescence. At the tenth passage, all cells bore tumor-associated antigen. Cultured cells that were injected s.c. into mice formed progressively growing nodules, the cells of which were morphologically indistinguishable from stromal cells of the original tumor; all cells retained tumor-associated antigen, but antigenic density had decreased to about one-seventh of the value found originally. No giant cells were present in the nodules.", "contents": "Quantitative immunofluorescence studies of the tumor antigen-bearing cell in giant cell tumor of bone and osteogenic sarcoma. Tumor-associated antigen was found by reacting sera from two patients with giant cell tumor of bone with cells derived from their tumors, using autologous serum as intermediate reactant and fluorescein-conjugated goat anti-human IgG as final reactant. Approximately 40% of the plump, spindle-shaped cells that formed the background stroma of these tumors possessed the antigen; however, it was not present on giant cells. Fluorescence was much greater than that on similarly stained cells from 4 osteogenic sarcomas, suggesting that the antigenic density on cells from giant cell tumor was greater than that on cells from osteogenic sarcoma. Antibodies in sera from giant cell tumor patients and osteogenic sarcoma patients showed specific cross-reactivity. Stromal cells of giant cell tumors were established in culture and retained tumor-associated antigen, whereas giant cells failed to divide and detached from the flask within two weeks. Intensity of fluorescence (antigenic density) decreased with progressive passage levels, but a larger percentage of cells showed fluorescence. At the tenth passage, all cells bore tumor-associated antigen. Cultured cells that were injected s.c. into mice formed progressively growing nodules, the cells of which were morphologically indistinguishable from stromal cells of the original tumor; all cells retained tumor-associated antigen, but antigenic density had decreased to about one-seventh of the value found originally. No giant cells were present in the nodules."}
{"id": "PMID:1097110", "title": "Cell-mediated antitumor immunity in breast cancer patients evaluated by antigen-induced  leukocyte adherence inhibition in test tubes.", "content": "Tumor antigen-induced inhibition of leukocyte adherence was adapted and modified for use in glass test tubes for the study of cell-mediated antitumor immunity to human adenocarcinoma of the breast. Peripheral blood leukocytes from 40 to 47 patients with proven breast cancer responded to an antigenic extract of breast cancer with significant leukocyte adherence inhibition, whereas only 2 of 32 controls showed a response. Further, 7 patients with histologically proven benign breast disease did not react to the breast adenocarcinoma extract, indicating that only breast cancer patients have leukocytes sensitized  to the breast cancer antigen. The cell-mediated antitumor response of the breast cancer patient was dependent on the stage of the cancer, and patients with disseminated cancer had decreased responsiveness. In fact, 4 of 7 breast cancer patients who had no response in the assay had disseminated breast cancer. Also, surgery and irradiation depressed leukocyte adherence inhibition responsiveness. Chromatographic fractionation on Sepharose 4B of the breast cancer extract showed that the antigenic component was greater than 10(6) daltons. The responsive cell in the assay interacts directly with the tumor antigen, and as a result subsequent adherence to glass is inhibited. The assay described is a comparatively simple and sensitive technique for demonstrating cell-mediated antitumor immunity and appears to be immunologically specific.", "contents": "Cell-mediated antitumor immunity in breast cancer patients evaluated by antigen-induced  leukocyte adherence inhibition in test tubes. Tumor antigen-induced inhibition of leukocyte adherence was adapted and modified for use in glass test tubes for the study of cell-mediated antitumor immunity to human adenocarcinoma of the breast. Peripheral blood leukocytes from 40 to 47 patients with proven breast cancer responded to an antigenic extract of breast cancer with significant leukocyte adherence inhibition, whereas only 2 of 32 controls showed a response. Further, 7 patients with histologically proven benign breast disease did not react to the breast adenocarcinoma extract, indicating that only breast cancer patients have leukocytes sensitized  to the breast cancer antigen. The cell-mediated antitumor response of the breast cancer patient was dependent on the stage of the cancer, and patients with disseminated cancer had decreased responsiveness. In fact, 4 of 7 breast cancer patients who had no response in the assay had disseminated breast cancer. Also, surgery and irradiation depressed leukocyte adherence inhibition responsiveness. Chromatographic fractionation on Sepharose 4B of the breast cancer extract showed that the antigenic component was greater than 10(6) daltons. The responsive cell in the assay interacts directly with the tumor antigen, and as a result subsequent adherence to glass is inhibited. The assay described is a comparatively simple and sensitive technique for demonstrating cell-mediated antitumor immunity and appears to be immunologically specific."}
{"id": "PMID:1097111", "title": "Selective cleavage of glycosidic linkages: studies with the O-specific polysaccharide from Shigella dysenteriae type 3.", "content": "Treatment of the O-specific polysaccharide from Shigella dysenteriae Type 3 with hydrazine in the presence of hydrazine sulphate resulted in quantitative N-deacetylation with the formation of a modified polysaccharide containing free amino groups.  Oxidation of the modified polysaccharide with periodate did not destroy the 2-amino-2-deoxygalactose residues, thus indicating that they were substituted at position 3.  Acid hydrolysis of the modified polysaccharide afforded 3-O-(2-amino-2-deoxy-beta-D-galactopyranosyl)-D-galactose, which was identified as the N-acetyl derivative.  Deamination of the modified polysaccharide with nitrous acid cleaved the 2-amino-2-deoxy-D-galactopyranosyl linkages to give a pentasaccharide as the major product, which appeared to be the modified chemical repeating unit of the O-specific polysaccharide.", "contents": "Selective cleavage of glycosidic linkages: studies with the O-specific polysaccharide from Shigella dysenteriae type 3. Treatment of the O-specific polysaccharide from Shigella dysenteriae Type 3 with hydrazine in the presence of hydrazine sulphate resulted in quantitative N-deacetylation with the formation of a modified polysaccharide containing free amino groups.  Oxidation of the modified polysaccharide with periodate did not destroy the 2-amino-2-deoxygalactose residues, thus indicating that they were substituted at position 3.  Acid hydrolysis of the modified polysaccharide afforded 3-O-(2-amino-2-deoxy-beta-D-galactopyranosyl)-D-galactose, which was identified as the N-acetyl derivative.  Deamination of the modified polysaccharide with nitrous acid cleaved the 2-amino-2-deoxy-D-galactopyranosyl linkages to give a pentasaccharide as the major product, which appeared to be the modified chemical repeating unit of the O-specific polysaccharide."}
{"id": "PMID:1097112", "title": "The relationship of hypertension to coronary, aortic, and iliofemoral atherosclerosis.", "content": "Evidence from actuarial statistics, epidemiologic studies, and laboratory experiments clearly indicates that hypertension has an accelerating effect on atherogenesis, and this atherogenesis appears to be a graded function of elevated intra-arterial pressure. The fact that atherosclerosis occurs preferentially in the abdominal aorta and iliofemoral arteries seems likely to result from the pressure augmentation by reflected pulse waves that is intensified by vasoconstriction and also by the increase in hydrostatis pressure that results from gravitational stress during standing. Vasoconstriction is a characteristic of hypertension and occurs also with upright posture. The predilection of the coronary epicardial vessels for atherosclerosis seems likely to relate to subtle pressure-volume changes in these arteries as a result of this vascular bed being in the highest pressure area of the arterial system and because intramyocardial arterial branches are completely occluded during systole. The possibility is presented that hypertension accelerates atherosclerosis because it is a metabolic determinant of the multifunctional arterial smooth muscle cells which have the potential for forming collagen and mucopolysaccharides as well as phospholipid.", "contents": "The relationship of hypertension to coronary, aortic, and iliofemoral atherosclerosis. Evidence from actuarial statistics, epidemiologic studies, and laboratory experiments clearly indicates that hypertension has an accelerating effect on atherogenesis, and this atherogenesis appears to be a graded function of elevated intra-arterial pressure. The fact that atherosclerosis occurs preferentially in the abdominal aorta and iliofemoral arteries seems likely to result from the pressure augmentation by reflected pulse waves that is intensified by vasoconstriction and also by the increase in hydrostatis pressure that results from gravitational stress during standing. Vasoconstriction is a characteristic of hypertension and occurs also with upright posture. The predilection of the coronary epicardial vessels for atherosclerosis seems likely to relate to subtle pressure-volume changes in these arteries as a result of this vascular bed being in the highest pressure area of the arterial system and because intramyocardial arterial branches are completely occluded during systole. The possibility is presented that hypertension accelerates atherosclerosis because it is a metabolic determinant of the multifunctional arterial smooth muscle cells which have the potential for forming collagen and mucopolysaccharides as well as phospholipid."}
{"id": "PMID:1097115", "title": "Metallic copper-induced granulocyte exudation in the study of granulocytopoiesis.", "content": "C3H/HeHa mice implanted with rods of metallic copper (CR) or glass (GR) exudate neutrophil granulocytes and mononuclear cells into the site of rod implant (peritoneal cavity). Exudation in CR mice is substantially greater than in GR animals. In CR mice there is an impressive stimulation of myelopoiesis measured in the femoral marrow subsequent to the initial accumulation in the peritoneal cavity. Serum levels of colony stimulating activity (CSA), an in vitro myeloproliferative stimulating activity, are elevated in such animals, as are femoral marrow agarcolony forming cells (CFC). The procedures is useful to the study of myelopoiesis and myelopoietic regulatory mechanisms.", "contents": "Metallic copper-induced granulocyte exudation in the study of granulocytopoiesis. C3H/HeHa mice implanted with rods of metallic copper (CR) or glass (GR) exudate neutrophil granulocytes and mononuclear cells into the site of rod implant (peritoneal cavity). Exudation in CR mice is substantially greater than in GR animals. In CR mice there is an impressive stimulation of myelopoiesis measured in the femoral marrow subsequent to the initial accumulation in the peritoneal cavity. Serum levels of colony stimulating activity (CSA), an in vitro myeloproliferative stimulating activity, are elevated in such animals, as are femoral marrow agarcolony forming cells (CFC). The procedures is useful to the study of myelopoiesis and myelopoietic regulatory mechanisms."}
{"id": "PMID:1097116", "title": "A non-destructive method for measuring intestinal cell transit time.", "content": "Villous exfoliation of intestinal epithelial cells, which had been previously labeled in the crypt with 125I-iododeoxy-uridine, can be detected by thyroidal accumulation of the liberated 125I-. The latter can be monitored externally. The interval between labeled precursor injection and the steep portion of the thyroid activity accumulation curve corresponds to the intestinal cell transit time, as measured by destructive techniques, in three animal species. The technique allows estimations of intestinal cell transit time to be made on an individual basis, and can be expeditiously applied to large animals. Very small tracer doses are required for detection.", "contents": "A non-destructive method for measuring intestinal cell transit time. Villous exfoliation of intestinal epithelial cells, which had been previously labeled in the crypt with 125I-iododeoxy-uridine, can be detected by thyroidal accumulation of the liberated 125I-. The latter can be monitored externally. The interval between labeled precursor injection and the steep portion of the thyroid activity accumulation curve corresponds to the intestinal cell transit time, as measured by destructive techniques, in three animal species. The technique allows estimations of intestinal cell transit time to be made on an individual basis, and can be expeditiously applied to large animals. Very small tracer doses are required for detection."}
{"id": "PMID:1097117", "title": "Influence of castration on incorporation of exogenous peroxidase into \"synaptic\" vesicles of the median eminence. II. The perivascular part of the palisade zone in male rats.", "content": "In the median eminence of male rats, nerve profiles in the immediate vicinity of portal capillaries have been divided into 4 categories on the basis of their vesicular content: profiles a with agranular \"synaptic\" vesicles of about 50 nm, b with similar agranular vesicles and also with granular vesicles of mainly 60-140 nm, c with granular vesicles alone and d without vesicles. Twenty-four hours after castration, the percentage of profiles of category a was significantly increased when compared with sham-operated animals, whereas the percentage of profiles of category b was significantly decreased. After intravenous injection of exogenous peroxidase, especially the nerve profiles located in direct contact with the outer basement membrane of the portal capillaries contained peroxidase positive \"synaptic\" vesicles. Injection off peroxidase after castration resulted in a significant increase in the percentage of nerve profiles containing both peroxidase positive and peroxidase negative \"synaptic\" vesicles (category a). It is suggested that, in certain nerve terminals, castration may lead to release off the content of granular vesicles, which may contain gonadotropin releasing factor. This release then may cause that nerve terminals with agranular vesicles and granular vesicles (category b) change in their vesicular content and are therefore classified as terminals with only agranular vesicles (category a). An increased turnover rate in the ultrastructurally affected terminals may be reflected in increased uptake of exogenous peroxidase. The observations imply that certain neurones projecting to portal capillaries in the median eminence are, directly or indirectly, sensitive to changes in the level of gonadal steroids.", "contents": "Influence of castration on incorporation of exogenous peroxidase into \"synaptic\" vesicles of the median eminence. II. The perivascular part of the palisade zone in male rats. In the median eminence of male rats, nerve profiles in the immediate vicinity of portal capillaries have been divided into 4 categories on the basis of their vesicular content: profiles a with agranular \"synaptic\" vesicles of about 50 nm, b with similar agranular vesicles and also with granular vesicles of mainly 60-140 nm, c with granular vesicles alone and d without vesicles. Twenty-four hours after castration, the percentage of profiles of category a was significantly increased when compared with sham-operated animals, whereas the percentage of profiles of category b was significantly decreased. After intravenous injection of exogenous peroxidase, especially the nerve profiles located in direct contact with the outer basement membrane of the portal capillaries contained peroxidase positive \"synaptic\" vesicles. Injection off peroxidase after castration resulted in a significant increase in the percentage of nerve profiles containing both peroxidase positive and peroxidase negative \"synaptic\" vesicles (category a). It is suggested that, in certain nerve terminals, castration may lead to release off the content of granular vesicles, which may contain gonadotropin releasing factor. This release then may cause that nerve terminals with agranular vesicles and granular vesicles (category b) change in their vesicular content and are therefore classified as terminals with only agranular vesicles (category a). An increased turnover rate in the ultrastructurally affected terminals may be reflected in increased uptake of exogenous peroxidase. The observations imply that certain neurones projecting to portal capillaries in the median eminence are, directly or indirectly, sensitive to changes in the level of gonadal steroids."}
{"id": "PMID:1097118", "title": "Brain tissue transplanted to the anterior chamber of the eye: 3. Substitution of lacking central noradrenaline input by host iris sympathetic fibers in the isolated cerebral cortex developed in oculo.", "content": "Fetal parietal cerebral cortex was homologously transplanted to the anterior chambers of the eyes of adult rats. The transplants got vascularized, proliferated, as measured by in vivo stereoscopic inspections, and differentiated into brain tissue similar to cortex cerebri in situ and survived for long times, greater than 41/2 months. Fibers from the intact sympathetic adrenergic ground plexus of the iris were able to innervate the transplants in an organotypic way regarding fluorescence morphology, pattern of distribution of the nerve terminals and, to a certain extent, density of innervation, the only variable parameter being density of innervation. Thus, in unpretreated or MAO inhibited transplants only rather few to scattered terminals could be found, while after preincubation in 10(-5)M alpha-methyl-noradrenaline the number of visible terminals was normal or slightly less than normal, as compared to cortex cerebri in situ. When superior cervical ganglia (SCG) were transplanted together with fetal cortex tissue to sympathetically denervated eyes the ingrowth in the cortex tissue was similar to that after single cortex transplantation combined with 5 day old SCG, while a marked hyperinnervation was encountered when combined with adult SCG. It is concluded that the developing cortex cerebri, deprived of its normal CNS source of adrenergic nerves, is able to receive sympathetic adrenergic nerves from the iris in an organotypic way upon transplantation to the anterior chamber of the eye.", "contents": "Brain tissue transplanted to the anterior chamber of the eye: 3. Substitution of lacking central noradrenaline input by host iris sympathetic fibers in the isolated cerebral cortex developed in oculo. Fetal parietal cerebral cortex was homologously transplanted to the anterior chambers of the eyes of adult rats. The transplants got vascularized, proliferated, as measured by in vivo stereoscopic inspections, and differentiated into brain tissue similar to cortex cerebri in situ and survived for long times, greater than 41/2 months. Fibers from the intact sympathetic adrenergic ground plexus of the iris were able to innervate the transplants in an organotypic way regarding fluorescence morphology, pattern of distribution of the nerve terminals and, to a certain extent, density of innervation, the only variable parameter being density of innervation. Thus, in unpretreated or MAO inhibited transplants only rather few to scattered terminals could be found, while after preincubation in 10(-5)M alpha-methyl-noradrenaline the number of visible terminals was normal or slightly less than normal, as compared to cortex cerebri in situ. When superior cervical ganglia (SCG) were transplanted together with fetal cortex tissue to sympathetically denervated eyes the ingrowth in the cortex tissue was similar to that after single cortex transplantation combined with 5 day old SCG, while a marked hyperinnervation was encountered when combined with adult SCG. It is concluded that the developing cortex cerebri, deprived of its normal CNS source of adrenergic nerves, is able to receive sympathetic adrenergic nerves from the iris in an organotypic way upon transplantation to the anterior chamber of the eye."}
{"id": "PMID:1097119", "title": "Encapsulation of implanted foreign bodies by hemocytes in Locusta migratoria and Melolontha melolontha.", "content": "Sterile fragments of cellophane, cat-gut, cotton, or beef muscle implanted into the body cavity of Locusta migratoria and Melolontha melolontha are immediately surrounded by granular hemocytes which form a multi-layered capsule around each foreign body. The cytoplasm of the granular hemocytes of the outer zone of the capsule in both species shows an accumulation of fibrous material (microtubules and microfilaments). None of the implanted material appeared to be degraded.", "contents": "Encapsulation of implanted foreign bodies by hemocytes in Locusta migratoria and Melolontha melolontha. Sterile fragments of cellophane, cat-gut, cotton, or beef muscle implanted into the body cavity of Locusta migratoria and Melolontha melolontha are immediately surrounded by granular hemocytes which form a multi-layered capsule around each foreign body. The cytoplasm of the granular hemocytes of the outer zone of the capsule in both species shows an accumulation of fibrous material (microtubules and microfilaments). None of the implanted material appeared to be degraded."}
{"id": "PMID:1097120", "title": "Changes in glia-neuron relationships in cell cultures of spinal ganglia caused by puromycin.", "content": "Dorsal root ganglia of chick embryos were cultured for one to four weeks on Maximow-slides. Puromycin was added to cultures for a pulse of 30' in a dose of 100 mug/ml medium. Particular interest was given to the ultrastructural features of the glia-neuron relations. Puromycin caused a shrinkage of the glial processes and consequently the continous glial envelope of the spinal ganglion neurons disappeared. These changes of glia-neuron contacts were reversible after a few days when puromycin was withdrawn from the medium. The newly formed glial sheaths around the neurons were comparable to those in untreated cultures and to those in vivo. The effects of an inhibition of protein synthesis caused by puromycin are discussed in relation to the effects on the cell motility, on the renewal of cell membranes and on the formation of cell contacts in nervous tissue.", "contents": "Changes in glia-neuron relationships in cell cultures of spinal ganglia caused by puromycin. Dorsal root ganglia of chick embryos were cultured for one to four weeks on Maximow-slides. Puromycin was added to cultures for a pulse of 30' in a dose of 100 mug/ml medium. Particular interest was given to the ultrastructural features of the glia-neuron relations. Puromycin caused a shrinkage of the glial processes and consequently the continous glial envelope of the spinal ganglion neurons disappeared. These changes of glia-neuron contacts were reversible after a few days when puromycin was withdrawn from the medium. The newly formed glial sheaths around the neurons were comparable to those in untreated cultures and to those in vivo. The effects of an inhibition of protein synthesis caused by puromycin are discussed in relation to the effects on the cell motility, on the renewal of cell membranes and on the formation of cell contacts in nervous tissue."}
{"id": "PMID:1097121", "title": "Ependymal absorption of luteinizing hormone-releasing hormone injected into the third ventricle of the rat.", "content": "LRH injected into the third ventricle induced increases of serum LH and FSH in the rat. Following injection of LRH-125I into the third ventricle, autoradiographic grains were concentrated in the ependymal cell bodies and ependymal processes in the median eminence. Aggregations of grains were also found in the capillaries of the portal vessels, and in both sinusoids and parenchymal cells of the adenohypophysis. These findings show that the ependymal cells represent a link between the cerebrospinal fluid and the portal blood.", "contents": "Ependymal absorption of luteinizing hormone-releasing hormone injected into the third ventricle of the rat. LRH injected into the third ventricle induced increases of serum LH and FSH in the rat. Following injection of LRH-125I into the third ventricle, autoradiographic grains were concentrated in the ependymal cell bodies and ependymal processes in the median eminence. Aggregations of grains were also found in the capillaries of the portal vessels, and in both sinusoids and parenchymal cells of the adenohypophysis. These findings show that the ependymal cells represent a link between the cerebrospinal fluid and the portal blood."}
{"id": "PMID:1097122", "title": "Extra-adrenal chromaffin cells grown in tissue culture.", "content": "1. Extra-adrenal chromaffin cells from adult frogs were grown in tissue culture and their morphology and behaviour observed with both light and electron microscopy. 2. Two types of chromaffin cells were distinguished: Type A cells contain large, electron dense vesicles (2000-6000 A) and are equated to Type I chromaffin cells seen in vivo, i.e. they contain noradrenaline; Type B cells contain smaller vesicles (700-2000 A) which are incompletely filled with an electron dense material and are equated to Type III chromaffin cells seen in vivo, i.e. cells depleted of their catecholamines by stimulation. No cells comparable to Types II and IV cells in vivo were seen. 3. Close associations between the cultured chromaffin cells and sympathetic neurons were observed with the light microscope, but no examples of synaptic structures were seen in the material examined with electron microscopy in this study.", "contents": "Extra-adrenal chromaffin cells grown in tissue culture. 1. Extra-adrenal chromaffin cells from adult frogs were grown in tissue culture and their morphology and behaviour observed with both light and electron microscopy. 2. Two types of chromaffin cells were distinguished: Type A cells contain large, electron dense vesicles (2000-6000 A) and are equated to Type I chromaffin cells seen in vivo, i.e. they contain noradrenaline; Type B cells contain smaller vesicles (700-2000 A) which are incompletely filled with an electron dense material and are equated to Type III chromaffin cells seen in vivo, i.e. cells depleted of their catecholamines by stimulation. No cells comparable to Types II and IV cells in vivo were seen. 3. Close associations between the cultured chromaffin cells and sympathetic neurons were observed with the light microscope, but no examples of synaptic structures were seen in the material examined with electron microscopy in this study."}
{"id": "PMID:1097124", "title": "Temporal order in yeast chromosome replication.", "content": "Previous work with bacteria has shown that a gene is maximally sensitive to mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NG) at the time it is being replicated. NG was used to test for temporal order in the replication of the genome of the unicellular eucaryote, Saccaromyces cerevisiae. Yeast cells growing exponentially were more sensitive to mutagenesis by NG than cells in which DNA synthesis had been inhibited. Further, in a synchronized population of cells, individual genetic markers exhibited maximum sensitivity to muta-genesis at distinct limited intervals within the DNA synthesis period. The peaks of sensitivity are interpreted as reflecting the times of replication of different genes. Since markers for five genes on four different chromosomes showed discrete periods of maximum sensitivity, it is likely that temporal ordering of replication exists for most genes in the yeast genome. These results imply that sites for initiation of DNA replication occur at fairly specific regions along yeast chromosomal DNA moleucles, and are activated at predetermined times in the DNA synthesis period.", "contents": "Temporal order in yeast chromosome replication. Previous work with bacteria has shown that a gene is maximally sensitive to mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NG) at the time it is being replicated. NG was used to test for temporal order in the replication of the genome of the unicellular eucaryote, Saccaromyces cerevisiae. Yeast cells growing exponentially were more sensitive to mutagenesis by NG than cells in which DNA synthesis had been inhibited. Further, in a synchronized population of cells, individual genetic markers exhibited maximum sensitivity to muta-genesis at distinct limited intervals within the DNA synthesis period. The peaks of sensitivity are interpreted as reflecting the times of replication of different genes. Since markers for five genes on four different chromosomes showed discrete periods of maximum sensitivity, it is likely that temporal ordering of replication exists for most genes in the yeast genome. These results imply that sites for initiation of DNA replication occur at fairly specific regions along yeast chromosomal DNA moleucles, and are activated at predetermined times in the DNA synthesis period."}
{"id": "PMID:1097127", "title": "[How can forensic medicine assist historical findings].", "content": "The authors wanted to demonstrate in their present paper that forensic medicine and its modern methods can help to elucidate some historical findings not only as regards mechanisms of injury but in the first member's of the dynasty of Premysl also by evidence of group appurtenance using the two-phase and two-circle system.", "contents": "[How can forensic medicine assist historical findings]. The authors wanted to demonstrate in their present paper that forensic medicine and its modern methods can help to elucidate some historical findings not only as regards mechanisms of injury but in the first member's of the dynasty of Premysl also by evidence of group appurtenance using the two-phase and two-circle system."}
{"id": "PMID:1097129", "title": "Effect of norepinephrine and fluid administration on pulmonary extravascular water volume in dogs.", "content": "The effects of norepinephrine-induced vasoconstriction on pulmonary extravascular water volume (PEWV) and central volume were studied before and after intravenous volume expansion with 5% dextrose and water (20 ml/kg body weight). PEWV was measured by double isotope dilution and gravimetric analysis and assessed by electron photomicrography. Comparisons were made with saline-treated control dogs. Thirty-six dogs were studied after they had been anesthetized with sodium pentobarbital. PEWV determined by indicator dilution increased during norepinephrine infusion, especially after volume expansion; in contrast, PEWV in control dogs was constant despite similar increases in pulmonary arterial and left atrial pressures. However, measurements of PEWV by gravimetric analysis and inspection of electron photomicrographs failed to reveal extravascular accumulation of water in vasoconstricted dogs. Thus, it was concluded that the increased PEWV measured by indicator dilution in norepinephrine-treated dogs compared with that in saline-treated dogs reflects more complete perfusion of pulmonary capillaries and access of the diffusible indicator to additional lung tissues and spaces. Improved capillary filling presumably is attributable to peripheral vasoconstriction and redistribution of blood into the thorax. PEWV determined by gravimetric analysis in dogs that had had open-chest procedures for lung biopsy and electron photomicrography was significantly less than that in dogs with their chests closed throughout the experiments. Although the mechanism for the water loss in unknown, the difference must be recognized when the results from various types of experiments are compared.", "contents": "Effect of norepinephrine and fluid administration on pulmonary extravascular water volume in dogs. The effects of norepinephrine-induced vasoconstriction on pulmonary extravascular water volume (PEWV) and central volume were studied before and after intravenous volume expansion with 5% dextrose and water (20 ml/kg body weight). PEWV was measured by double isotope dilution and gravimetric analysis and assessed by electron photomicrography. Comparisons were made with saline-treated control dogs. Thirty-six dogs were studied after they had been anesthetized with sodium pentobarbital. PEWV determined by indicator dilution increased during norepinephrine infusion, especially after volume expansion; in contrast, PEWV in control dogs was constant despite similar increases in pulmonary arterial and left atrial pressures. However, measurements of PEWV by gravimetric analysis and inspection of electron photomicrographs failed to reveal extravascular accumulation of water in vasoconstricted dogs. Thus, it was concluded that the increased PEWV measured by indicator dilution in norepinephrine-treated dogs compared with that in saline-treated dogs reflects more complete perfusion of pulmonary capillaries and access of the diffusible indicator to additional lung tissues and spaces. Improved capillary filling presumably is attributable to peripheral vasoconstriction and redistribution of blood into the thorax. PEWV determined by gravimetric analysis in dogs that had had open-chest procedures for lung biopsy and electron photomicrography was significantly less than that in dogs with their chests closed throughout the experiments. Although the mechanism for the water loss in unknown, the difference must be recognized when the results from various types of experiments are compared."}
{"id": "PMID:1097131", "title": "Right ventricular dysfunction detected by gated scintiphotography in patients with acute inferior myocardial infarction.", "content": "Twenty-seven patients with acute myocardial infarction not complicated by cardiogenic shock and ten normal volunteers were studied with gated cardiac blood pool scans. The ratio right vetricular area/left ventricular area (RVA/LVA) determined from the left anterior oblique end-diastolic scans was examined. The ratio was 1.11 +/- .06 in the normal volunteers. In patients with anterior infarction the ratio fell to 0.75 +/- .12 (P less than .05) due to left ventricular enlargement. In those with inferior infarction the ratio was 1.12 +/- .23 which was greater than in those with anterior infarction (P less than .05) due to enlargement of both the left and right ventricles. Six patients with cardiogenic shock, three with inferior and three with anterior infarction were studied. The three with anterior infarction had left ventricular enlargement and a decrease in the ratio of RVA/LVA to 0.62 while the three with inferior infarction had an increase in the ratio to 2.05 suggesting right ventricular dilatation and dysfunction. These studies suggest a high incidence of right ventricular dysfunction in patients with inferior myocardial infarction.", "contents": "Right ventricular dysfunction detected by gated scintiphotography in patients with acute inferior myocardial infarction. Twenty-seven patients with acute myocardial infarction not complicated by cardiogenic shock and ten normal volunteers were studied with gated cardiac blood pool scans. The ratio right vetricular area/left ventricular area (RVA/LVA) determined from the left anterior oblique end-diastolic scans was examined. The ratio was 1.11 +/- .06 in the normal volunteers. In patients with anterior infarction the ratio fell to 0.75 +/- .12 (P less than .05) due to left ventricular enlargement. In those with inferior infarction the ratio was 1.12 +/- .23 which was greater than in those with anterior infarction (P less than .05) due to enlargement of both the left and right ventricles. Six patients with cardiogenic shock, three with inferior and three with anterior infarction were studied. The three with anterior infarction had left ventricular enlargement and a decrease in the ratio of RVA/LVA to 0.62 while the three with inferior infarction had an increase in the ratio to 2.05 suggesting right ventricular dilatation and dysfunction. These studies suggest a high incidence of right ventricular dysfunction in patients with inferior myocardial infarction."}
{"id": "PMID:1097132", "title": "An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers.", "content": "An analytical anion-exchange procedure has been developed for the rapid separation of acid-soluble nucleotides (the so-called \"free\" or tissue nucleotides). It permits assay at low pressures (275-415 kPa; 40-60 psi) in less than 1 h on 10-cm columns of Aminex resins (conventional styrene-type anion exchangers) with alkaline citrate solutions as the eluent. Separation variables have been investigated, to determine optimum conditions for the routine analysis of samples containing tissue nucleotides. Also described here is a simple solvent-extraction procedure for removing HCIO4 or CCI3CO2H quantitatively from cell extracts that contain acid-soluble nucleotides: they are removed from aqueous acid solutions with a water-insoluble amine dissolved in a water-immiscible solvent.", "contents": "An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers. An analytical anion-exchange procedure has been developed for the rapid separation of acid-soluble nucleotides (the so-called \"free\" or tissue nucleotides). It permits assay at low pressures (275-415 kPa; 40-60 psi) in less than 1 h on 10-cm columns of Aminex resins (conventional styrene-type anion exchangers) with alkaline citrate solutions as the eluent. Separation variables have been investigated, to determine optimum conditions for the routine analysis of samples containing tissue nucleotides. Also described here is a simple solvent-extraction procedure for removing HCIO4 or CCI3CO2H quantitatively from cell extracts that contain acid-soluble nucleotides: they are removed from aqueous acid solutions with a water-insoluble amine dissolved in a water-immiscible solvent."}
{"id": "PMID:1097133", "title": "Determination of hydroxyproline polypeptides in urine and blood serum by gel filtration.", "content": "Hydroxyproline-containing polypeptides were isolated from urine by filtration on polyacrylamide gel Bio-Gel P-2. This fraction was quantitatively and qualitatively similar to the nondialysable hydroxyproline in urines of normal adult subjects, patients with chronic uremia, and with primary or secondary hyperparathyroidism. Hydroxyproline polypeptides isolated from urines of patients with extensive Paget's disease or from normal growing subjects contained a group of smaller polypeptides slightly retarded on Bio-Gel P-2 which were not retained by the dialysis membrane Spectrapor-TM. In these two groups of subjects the values of hydroxyproline polypeptides were 35-45 percent higher than those of dialysable hydroxyproline. Increased excretion of hydroxyproline polypeptides was found in chronic uremia, primary and secondary hyperparathyroidism, Paget's bone disease and in normal growing subjects. With the notable exception of chronic uremia the excretion of hydroxyproline polypeptides paralleled that of total and oligopeptidic hydroxyproline. In some cases of chronic uremia the polypeptidic hydroxyproline represented the predominant fraction. On long columns of Bio-Gel P-6 hydroxyproline polypeptides separated into several peaks. The chromatographic pattern was rather characteristic for chronic uremia, secondary hyperparathyroidism, Paget's bone disease and physiological growth. Ahydroxyproline fraction of similar chromatographic properties was isolated from blood serum after deproteinisation with heat or ethanol at pH 4.8. High serum levels of this hydroxyproline fraction were observed in pathological conditions with increased urinary excretion of polypeptidic and nondialysable hydroxyproline.", "contents": "Determination of hydroxyproline polypeptides in urine and blood serum by gel filtration. Hydroxyproline-containing polypeptides were isolated from urine by filtration on polyacrylamide gel Bio-Gel P-2. This fraction was quantitatively and qualitatively similar to the nondialysable hydroxyproline in urines of normal adult subjects, patients with chronic uremia, and with primary or secondary hyperparathyroidism. Hydroxyproline polypeptides isolated from urines of patients with extensive Paget's disease or from normal growing subjects contained a group of smaller polypeptides slightly retarded on Bio-Gel P-2 which were not retained by the dialysis membrane Spectrapor-TM. In these two groups of subjects the values of hydroxyproline polypeptides were 35-45 percent higher than those of dialysable hydroxyproline. Increased excretion of hydroxyproline polypeptides was found in chronic uremia, primary and secondary hyperparathyroidism, Paget's bone disease and in normal growing subjects. With the notable exception of chronic uremia the excretion of hydroxyproline polypeptides paralleled that of total and oligopeptidic hydroxyproline. In some cases of chronic uremia the polypeptidic hydroxyproline represented the predominant fraction. On long columns of Bio-Gel P-6 hydroxyproline polypeptides separated into several peaks. The chromatographic pattern was rather characteristic for chronic uremia, secondary hyperparathyroidism, Paget's bone disease and physiological growth. Ahydroxyproline fraction of similar chromatographic properties was isolated from blood serum after deproteinisation with heat or ethanol at pH 4.8. High serum levels of this hydroxyproline fraction were observed in pathological conditions with increased urinary excretion of polypeptidic and nondialysable hydroxyproline."}
{"id": "PMID:1097134", "title": "Automated assay of N-acetyl-beta-glucosaminidase in normal and pathological human urine.", "content": "An automated method for the assay of N-acetyl-beta-glucosaminidase in human urine is described and a normal range of urinary N-acetyl-beta-glucosaminidase activity is reported. The automated method has been used routinely over a twelve month period in two London hospitals for monitoring the excretion of N-acetyl-beta-glucosaminidase in renal transplant patients. The abnormal elevation of urinary N-acetyl-beta-glucosaminidase provides an early warning of rejection by indicating the presence of renal parenchymal damage. The automated assay of urinary N-acetyl-beta-glucosaminidase may also be used in screening for the presence of renal disease.", "contents": "Automated assay of N-acetyl-beta-glucosaminidase in normal and pathological human urine. An automated method for the assay of N-acetyl-beta-glucosaminidase in human urine is described and a normal range of urinary N-acetyl-beta-glucosaminidase activity is reported. The automated method has been used routinely over a twelve month period in two London hospitals for monitoring the excretion of N-acetyl-beta-glucosaminidase in renal transplant patients. The abnormal elevation of urinary N-acetyl-beta-glucosaminidase provides an early warning of rejection by indicating the presence of renal parenchymal damage. The automated assay of urinary N-acetyl-beta-glucosaminidase may also be used in screening for the presence of renal disease."}
{"id": "PMID:1097135", "title": "Sex hormone levels and gonadotrophin release in the polycystic ovary syndrome.", "content": "The response to synthetic luteinizing hormone-releasing hormone was studied in eighteen patients with the polycystic ovary syndrome. The release of follicle-stimulating hormone was similar to that found in normal women. The mean response of luteinizing hormone was similar to that found in the luteal phase, but significantly greater (P less than 0-02) than that found in the early follicular phase of the normal menstrual cycle. Basal serum levels of FSH and LH, estimated in twenty-five patients, were similar to those found in normal subjects. The sex hormone binding globulin capacity was reduced in twenty-four of them. Basal serum testosterone levels were elevated in twelve of twenty-two patients and the mean level was significantly greater (P less than 0-01) than the mean level of normal women. Basal serum androstenecione levels were elevated in nine of twenty-two patients and the mean level was also significantly greater (P leess than 0-001) between basal testosterone and LH levels. These data suggest the pituitary gland of patients with the polycystic ovary syndrome contains adequate amounts of LH but that the ovulatory surge of LH which occurs in normal women is inhibited by testosterone acting on either the pituitary or, more probably, on the hypothalamus.", "contents": "Sex hormone levels and gonadotrophin release in the polycystic ovary syndrome. The response to synthetic luteinizing hormone-releasing hormone was studied in eighteen patients with the polycystic ovary syndrome. The release of follicle-stimulating hormone was similar to that found in normal women. The mean response of luteinizing hormone was similar to that found in the luteal phase, but significantly greater (P less than 0-02) than that found in the early follicular phase of the normal menstrual cycle. Basal serum levels of FSH and LH, estimated in twenty-five patients, were similar to those found in normal subjects. The sex hormone binding globulin capacity was reduced in twenty-four of them. Basal serum testosterone levels were elevated in twelve of twenty-two patients and the mean level was significantly greater (P less than 0-01) than the mean level of normal women. Basal serum androstenecione levels were elevated in nine of twenty-two patients and the mean level was also significantly greater (P leess than 0-001) between basal testosterone and LH levels. These data suggest the pituitary gland of patients with the polycystic ovary syndrome contains adequate amounts of LH but that the ovulatory surge of LH which occurs in normal women is inhibited by testosterone acting on either the pituitary or, more probably, on the hypothalamus."}
{"id": "PMID:1097136", "title": "The effect of oestrogen pretreatment on subsequent response to luteinizing hormone releasing hormone in normal women.", "content": "Twenty-two normal, regularly menstruating female subjects had an LHRH test performed before and after pretreatment with 0.5 mg, 1 mg. or 2.5 mg of oestradiol benzoate during the follicular phase of their menstrual cyclees (days 4-8). Two further women acted as controls and received no oestrogen; they showed identical responses for both LH and FSH release when LHRH tests were performed at intervals of 48 h. Oestrogen pretreatment induced a biphasic effect upon subsequent LHRH response. Four subjects retested 20 h after 0.5 mg oestradiol benzoate showed either no change or a slight suppression of LH and FSH release. Fifteen of the eighteen women pretreated with oestradiol benzoate and retested 44 h later showed significantly increased LH release and fourteen significantly increased FSH release when compared to their control responses. The responses appeared to be dose related with a positive correlation between sum of LH increments and basal oestradiol levels (R = 0.61; P less than 0.001) and a similar correlation (r = 0.67; P less than 0.001) between sum of FSH increments and basal oestradiol levels. The physiological significance of this biphasic action of oestrogen upon pituitary sensitivity is discussed in relation to the control of the menstrual cycle.", "contents": "The effect of oestrogen pretreatment on subsequent response to luteinizing hormone releasing hormone in normal women. Twenty-two normal, regularly menstruating female subjects had an LHRH test performed before and after pretreatment with 0.5 mg, 1 mg. or 2.5 mg of oestradiol benzoate during the follicular phase of their menstrual cyclees (days 4-8). Two further women acted as controls and received no oestrogen; they showed identical responses for both LH and FSH release when LHRH tests were performed at intervals of 48 h. Oestrogen pretreatment induced a biphasic effect upon subsequent LHRH response. Four subjects retested 20 h after 0.5 mg oestradiol benzoate showed either no change or a slight suppression of LH and FSH release. Fifteen of the eighteen women pretreated with oestradiol benzoate and retested 44 h later showed significantly increased LH release and fourteen significantly increased FSH release when compared to their control responses. The responses appeared to be dose related with a positive correlation between sum of LH increments and basal oestradiol levels (R = 0.61; P less than 0.001) and a similar correlation (r = 0.67; P less than 0.001) between sum of FSH increments and basal oestradiol levels. The physiological significance of this biphasic action of oestrogen upon pituitary sensitivity is discussed in relation to the control of the menstrual cycle."}
{"id": "PMID:1097137", "title": "The LH response to LH releasing hormone in children with true isosexual precocious puberty treated with cyproterone acetate.", "content": "Fourteen girls and one boy with isosexual precocious puberty were submitted to LHRH stimulation tests, during and without therapy with cyproterone acetate. In addition, fourteen girls with isosexual precocious puberty not receiving any therapy were tested and served as controls. It was found that cyproterone acetate induces suppression of the responsiveness of the pituitary gland to secrete LH on LHRH stimulation. Daily oral therapy was found to be more effective than the regimen of intramuscular depot injections. These findings demonstrate that cyproterone acetate has an antigonadotrophic effect.", "contents": "The LH response to LH releasing hormone in children with true isosexual precocious puberty treated with cyproterone acetate. Fourteen girls and one boy with isosexual precocious puberty were submitted to LHRH stimulation tests, during and without therapy with cyproterone acetate. In addition, fourteen girls with isosexual precocious puberty not receiving any therapy were tested and served as controls. It was found that cyproterone acetate induces suppression of the responsiveness of the pituitary gland to secrete LH on LHRH stimulation. Daily oral therapy was found to be more effective than the regimen of intramuscular depot injections. These findings demonstrate that cyproterone acetate has an antigonadotrophic effect."}
{"id": "PMID:1097145", "title": "Allergic interstitial nephritis: report of a case with activation of complement by the alternate pathway.", "content": "An eighteen year old woman developed a streptococcal pharyngitis documented by throat culture. After two days of treatment with penicillin she developed arthritis and abdominal pain and within a week became anuric. Her renal biopsy revealed extensive interstitial nephritis without proliferative glomerular changes. Serum properdin levels were significantly reduced and immunofluorescence studies demonstrated properdin deposition within the glomeruli, C1, C4 and C2 were not depressed nor were they demonstrable within the glomeruli. These results support activation of complement apparently only by the alternate pathway, a finding heretofore noted only in membranoproliferative glomerulonephritis.", "contents": "Allergic interstitial nephritis: report of a case with activation of complement by the alternate pathway. An eighteen year old woman developed a streptococcal pharyngitis documented by throat culture. After two days of treatment with penicillin she developed arthritis and abdominal pain and within a week became anuric. Her renal biopsy revealed extensive interstitial nephritis without proliferative glomerular changes. Serum properdin levels were significantly reduced and immunofluorescence studies demonstrated properdin deposition within the glomeruli, C1, C4 and C2 were not depressed nor were they demonstrable within the glomeruli. These results support activation of complement apparently only by the alternate pathway, a finding heretofore noted only in membranoproliferative glomerulonephritis."}
{"id": "PMID:1097142", "title": "A simple combinatorial method for calculating genetic risks.", "content": "A method is presented whereby genetic counsellors can calculate genetic risks in a wide variety of circumstances using only arithmetics. The method is general in that it can handle such information as gene frequency, mutation rate, mode of inheritance, penetrance, pedigree to which the consultand belongs, phenotype of the consultand and his relatives, biochemical findings, etc. Each of the possible combinations of genotypes which may be present in the pedigree is listed and considered in turn. The method consists of a series of simple steps leading mechanically to the right answer.", "contents": "A simple combinatorial method for calculating genetic risks. A method is presented whereby genetic counsellors can calculate genetic risks in a wide variety of circumstances using only arithmetics. The method is general in that it can handle such information as gene frequency, mutation rate, mode of inheritance, penetrance, pedigree to which the consultand belongs, phenotype of the consultand and his relatives, biochemical findings, etc. Each of the possible combinations of genotypes which may be present in the pedigree is listed and considered in turn. The method consists of a series of simple steps leading mechanically to the right answer."}
{"id": "PMID:1097148", "title": "Interactions in man of delta-9-tetrahydrocannabinol. II. Cannabinol and cannabidiol.", "content": "Oral doses of delta-9-tetrahydrocannabinol (THC) 20 mg, combined with placebo or with 40 mg dses of cannabinol (CBN) and cannabidiol (CBD), were given to volunteers. The combination of THC with CBN produced no detectable changes in the quality, intensity, or duration of the effects of THC alone. The THC-CBD combination tended to delay onset and prolong effects of THC, while making them somewhat more intense. Even this interactive effect was slight, providing no reason to abandon the current practice of basing doses of marihuana for clinical studies solely on THC content.", "contents": "Interactions in man of delta-9-tetrahydrocannabinol. II. Cannabinol and cannabidiol. Oral doses of delta-9-tetrahydrocannabinol (THC) 20 mg, combined with placebo or with 40 mg dses of cannabinol (CBN) and cannabidiol (CBD), were given to volunteers. The combination of THC with CBN produced no detectable changes in the quality, intensity, or duration of the effects of THC alone. The THC-CBD combination tended to delay onset and prolong effects of THC, while making them somewhat more intense. Even this interactive effect was slight, providing no reason to abandon the current practice of basing doses of marihuana for clinical studies solely on THC content."}
{"id": "PMID:1097143", "title": "Potassium-sparing effect of amiloride in a diuretic factorial study in man.", "content": "1. The effects of amiloride (M, 20 mg/day), chlorothiazide (C, 1000 mg/day), ethacrynic acid (E, 100 MG/day) and frusemide (F, furosemide 80 mg/day, given alone and in combination, were investigated in eight patients in a 2(4) factorial study. Effects between blocks of four treatments in each sixteen-treatment replicate were confounded with higher interactions to allow for differences between early and late diuresis. 2. All patients exhibited marked diuresis, with significant mean increases in daily urinary sodium excretion (P smaller than 0.05) and urinary volume (P smaller than 0.01) induced by chlorothiazide, frusemide and ethacrynic acid (68, 69 and 38%; and 35, 40 and 34%, respectively). Amiloride appeared to be half to one-third as potent as the other diuretics. 3. Amiloride produced a significant potassium-sparing effect (P smaller than 0.01), reducing urinary potassium excretion by 30%, compared to significant urinary potassium wasting with frusemide (increase of 33%, P smaller than 0.01) and chlorothiazide (increase of 31%, P smaller than 0.05). 4. No adverse reactions occurred, but serum potassium levels twice rose to 6 mmol/l and subsided without additional therapy, and on one occasion fell to 2.5 mmol/l, requiring a potassium supplement for 4 days. 5. It was concluded from these factorial studies that amiloride is a mild diuretic and potent potassium-sparing agent acting independently and additively in combination with chlorothiazide, ethacrynic acid or frusemide. For the three latter diuretics, all two-drug interactions were simply additive also, without evidence of synergism or antagonism between drugs.", "contents": "Potassium-sparing effect of amiloride in a diuretic factorial study in man. 1. The effects of amiloride (M, 20 mg/day), chlorothiazide (C, 1000 mg/day), ethacrynic acid (E, 100 MG/day) and frusemide (F, furosemide 80 mg/day, given alone and in combination, were investigated in eight patients in a 2(4) factorial study. Effects between blocks of four treatments in each sixteen-treatment replicate were confounded with higher interactions to allow for differences between early and late diuresis. 2. All patients exhibited marked diuresis, with significant mean increases in daily urinary sodium excretion (P smaller than 0.05) and urinary volume (P smaller than 0.01) induced by chlorothiazide, frusemide and ethacrynic acid (68, 69 and 38%; and 35, 40 and 34%, respectively). Amiloride appeared to be half to one-third as potent as the other diuretics. 3. Amiloride produced a significant potassium-sparing effect (P smaller than 0.01), reducing urinary potassium excretion by 30%, compared to significant urinary potassium wasting with frusemide (increase of 33%, P smaller than 0.01) and chlorothiazide (increase of 31%, P smaller than 0.05). 4. No adverse reactions occurred, but serum potassium levels twice rose to 6 mmol/l and subsided without additional therapy, and on one occasion fell to 2.5 mmol/l, requiring a potassium supplement for 4 days. 5. It was concluded from these factorial studies that amiloride is a mild diuretic and potent potassium-sparing agent acting independently and additively in combination with chlorothiazide, ethacrynic acid or frusemide. For the three latter diuretics, all two-drug interactions were simply additive also, without evidence of synergism or antagonism between drugs."}
{"id": "PMID:1097150", "title": "Antihypertensive effect of a dopamine beta hydroxylase inhibitor, bupicomide: a comparison with hydralazine.", "content": "The cardiovascular effects of a new antihypertensive drug, bupicomide, were compared with those of hydralazine in 6 patients with mild to moderate hypertension. The mean supine arterial pressure of patients was reduced 15.2 mm Hg by bupicomide (900 to 2,000 mg/day) and 15.7 mm Hg by hydralazine (300 to 600 mg/day). Heart rate increased an average of 11.3 bpm during bupicomide and 14.5 bpm by hydralazine. Neither drug was associated with a postural decrease in mean arterial pressure. The heart rate response during maximum tolerated treadmill exercise was not diminished by either drug. Cardiac index was increased during administration of both drugs. Bupicomide and hydralazine reduced forearm vascular resistance, while renal blood flow and renal vascular resistance were not significantly modified. Evidence of equivalent augmentation of sympathetic nervous activity during administration of both drugs consisted of equal and significant increases in heart rate and urinary norepinephrine excretion, and decreases in duration of the pre-ejection period. The absolute values of these parameters were correlated with mean arterial pressure, which may indicate that the increase in sympathetic nervous activity was mediated by baroreceptor reflexes. Although bupicomide inhibits dopamine beta-hydroxylase, our results suggest that it is acting as a direct vasodilator.", "contents": "Antihypertensive effect of a dopamine beta hydroxylase inhibitor, bupicomide: a comparison with hydralazine. The cardiovascular effects of a new antihypertensive drug, bupicomide, were compared with those of hydralazine in 6 patients with mild to moderate hypertension. The mean supine arterial pressure of patients was reduced 15.2 mm Hg by bupicomide (900 to 2,000 mg/day) and 15.7 mm Hg by hydralazine (300 to 600 mg/day). Heart rate increased an average of 11.3 bpm during bupicomide and 14.5 bpm by hydralazine. Neither drug was associated with a postural decrease in mean arterial pressure. The heart rate response during maximum tolerated treadmill exercise was not diminished by either drug. Cardiac index was increased during administration of both drugs. Bupicomide and hydralazine reduced forearm vascular resistance, while renal blood flow and renal vascular resistance were not significantly modified. Evidence of equivalent augmentation of sympathetic nervous activity during administration of both drugs consisted of equal and significant increases in heart rate and urinary norepinephrine excretion, and decreases in duration of the pre-ejection period. The absolute values of these parameters were correlated with mean arterial pressure, which may indicate that the increase in sympathetic nervous activity was mediated by baroreceptor reflexes. Although bupicomide inhibits dopamine beta-hydroxylase, our results suggest that it is acting as a direct vasodilator."}
{"id": "PMID:1097151", "title": "Nonemergency use of slow infusions of diazoxide.", "content": "Slow infusions of diazoxide were administered to 10 hypertensive patients who had stable, nonaccelerated hypertension. The 10-min diazoxide infusion was associated with a 16% average reduction in arterial pressure, a 21% average increase in heart rate, a 16% average increase in cardiac output, and a 36% reduction in total peripheral resistance. These changes in hemodynamic parameters lasted for an average of 70 min. It was concluded that slow infusions of diazoxide produce a consistent and predictable antihypertensive effect in patients with stable, nonaccelerated hypertension. Slow infusions of diazoxide may have a limited use in nonemergency situations where an abrupt change in arterial pressure is to be avoided and a parenteral antihypertensive agent is needed.", "contents": "Nonemergency use of slow infusions of diazoxide. Slow infusions of diazoxide were administered to 10 hypertensive patients who had stable, nonaccelerated hypertension. The 10-min diazoxide infusion was associated with a 16% average reduction in arterial pressure, a 21% average increase in heart rate, a 16% average increase in cardiac output, and a 36% reduction in total peripheral resistance. These changes in hemodynamic parameters lasted for an average of 70 min. It was concluded that slow infusions of diazoxide produce a consistent and predictable antihypertensive effect in patients with stable, nonaccelerated hypertension. Slow infusions of diazoxide may have a limited use in nonemergency situations where an abrupt change in arterial pressure is to be avoided and a parenteral antihypertensive agent is needed."}
{"id": "PMID:1097152", "title": "Effect of dosage regimen on natriuretic response to furosemide.", "content": "We have examined the differences in urinary excretion of water, sodium, potassium, chloride, urea, and creatinine produced by different dosage regimens offurosemide in normal volunteers. Three oral dosage regimens were compared: 20 mg daily, 20 mg twice daily, and 40 mg daily. Furosemide, 20 mg, did not produce a significant weight loss, diuresis, or natriuresis in 12 normal subjects. With 40 there was a significant weight loss, diuresis, natriuresis, and chloruresis over 24 hr. Comparison of the divided regimen with 40 mg daily revealed significantly greater sodium excretion, and chloride excretion with 20 mg twice daily. The divided dosage regimen produced a different pattern of diuresis with most of the sodium and water excretion occurring after the second dose. These differences in response to different regimens are predictable from pharmacokinetic considerations and may have clinical significance.", "contents": "Effect of dosage regimen on natriuretic response to furosemide. We have examined the differences in urinary excretion of water, sodium, potassium, chloride, urea, and creatinine produced by different dosage regimens offurosemide in normal volunteers. Three oral dosage regimens were compared: 20 mg daily, 20 mg twice daily, and 40 mg daily. Furosemide, 20 mg, did not produce a significant weight loss, diuresis, or natriuresis in 12 normal subjects. With 40 there was a significant weight loss, diuresis, natriuresis, and chloruresis over 24 hr. Comparison of the divided regimen with 40 mg daily revealed significantly greater sodium excretion, and chloride excretion with 20 mg twice daily. The divided dosage regimen produced a different pattern of diuresis with most of the sodium and water excretion occurring after the second dose. These differences in response to different regimens are predictable from pharmacokinetic considerations and may have clinical significance."}
{"id": "PMID:1097153", "title": "Respiratory effects of nefopam.", "content": "A respiratory study has been done to compare the respiratory effects of a potent new analgesic, nefopam, to morphine.  Morphine was a potent respiratory depressant at therapeutic dosage while nefopam was not.", "contents": "Respiratory effects of nefopam. A respiratory study has been done to compare the respiratory effects of a potent new analgesic, nefopam, to morphine.  Morphine was a potent respiratory depressant at therapeutic dosage while nefopam was not."}
{"id": "PMID:1097154", "title": "Bioavailability of oral dexamethasone.", "content": "Bioavailabilities of dexamethasone tablets and elixir in man were evaluated by each of 3 model-independent methods of pharmacokinetic analysis employing plasma and urinary values as determined by radioimmune assay. There were no significant differences among the results determined by the 3 methods nor between the respective availabilities of the two formulations; the latter averaged 82.6 +/- 17.7% for the elixir formulation and 78.0 +/- 12.1% for tablets.", "contents": "Bioavailability of oral dexamethasone. Bioavailabilities of dexamethasone tablets and elixir in man were evaluated by each of 3 model-independent methods of pharmacokinetic analysis employing plasma and urinary values as determined by radioimmune assay. There were no significant differences among the results determined by the 3 methods nor between the respective availabilities of the two formulations; the latter averaged 82.6 +/- 17.7% for the elixir formulation and 78.0 +/- 12.1% for tablets."}
{"id": "PMID:1097158", "title": "Normal velopharyngeal function in speech.", "content": "What has been presented in the foregoing pages is the broad outline of a theory of velopharyngeal function in normal speech production. It is suggested that the velopharyngeal musculature functions in a predictably variable manner to provide predictably variable structural movements and positioning. The variability is predictable, based on the phenomenon of coarticulation, given knowledge of where the structures are, where they must go to produce a perceptually acceptable phonime, and where they must subsequently be for later phonemes. The theory is not complete, as will be discussed below. Nevertheless, it demonstrates the complexity of velopharyngeal function. Many variables are involved simultaneously in the movements of this mechanism, and it is not enough to consider a muscle, a structure, or a phoneme. The action of any muscle is influenced by the action of others in the system, the movements of any structure are influenced by movements of other structures, and any phoneme is influenced-sometimes heavily so-by phonemes around it. Thus, the central nervous system programming of oralization and nasalization gestures must be a complicated process. Certainly, the results of that programming are complicated.", "contents": "Normal velopharyngeal function in speech. What has been presented in the foregoing pages is the broad outline of a theory of velopharyngeal function in normal speech production. It is suggested that the velopharyngeal musculature functions in a predictably variable manner to provide predictably variable structural movements and positioning. The variability is predictable, based on the phenomenon of coarticulation, given knowledge of where the structures are, where they must go to produce a perceptually acceptable phonime, and where they must subsequently be for later phonemes. The theory is not complete, as will be discussed below. Nevertheless, it demonstrates the complexity of velopharyngeal function. Many variables are involved simultaneously in the movements of this mechanism, and it is not enough to consider a muscle, a structure, or a phoneme. The action of any muscle is influenced by the action of others in the system, the movements of any structure are influenced by movements of other structures, and any phoneme is influenced-sometimes heavily so-by phonemes around it. Thus, the central nervous system programming of oralization and nasalization gestures must be a complicated process. Certainly, the results of that programming are complicated."}
{"id": "PMID:1097162", "title": "A 10-year experience with subcutaneous mastectomy.", "content": "Subcutaneous mastectomy appears to remain a procedure of promise.  This 10-year experience indicated that it is feasible in at least 80 per cent of selected patients to successfully remove the breast parenchyma and subsequently reconstruct the breast without serious complications.  It is resonable to assume that as surgical technique and experience improve, the complication rate will diminish.  Furthermore, it is essential that better breast implants be devised and developed in the future.  This would clearly enhance all forms of cosmetic breast surgery.  The use of subcutaneous mastectomy as a prophylactic cancer procedure will parellel the improvement of implant breast reconstruction.  Whether subcutaneous mastectomy will measurably reduce the mortality rate of carcinoma of the breast will, of course, take a generation to determine.  However, I have no doubts that is it as justifiable and valuable surgical tool in the treatment of breast disease.  The concept of almost total breast biopsy has great merit in the discovery of occult carcinoma.  Clearly, caution should be exercised in the selection of cases for this modality, and further study must be devoted to develop diagnostic guidelines of ever-increasing precision to determine which breasts are potentially malignant and should be afforded the procedure.", "contents": "A 10-year experience with subcutaneous mastectomy. Subcutaneous mastectomy appears to remain a procedure of promise.  This 10-year experience indicated that it is feasible in at least 80 per cent of selected patients to successfully remove the breast parenchyma and subsequently reconstruct the breast without serious complications.  It is resonable to assume that as surgical technique and experience improve, the complication rate will diminish.  Furthermore, it is essential that better breast implants be devised and developed in the future.  This would clearly enhance all forms of cosmetic breast surgery.  The use of subcutaneous mastectomy as a prophylactic cancer procedure will parellel the improvement of implant breast reconstruction.  Whether subcutaneous mastectomy will measurably reduce the mortality rate of carcinoma of the breast will, of course, take a generation to determine.  However, I have no doubts that is it as justifiable and valuable surgical tool in the treatment of breast disease.  The concept of almost total breast biopsy has great merit in the discovery of occult carcinoma.  Clearly, caution should be exercised in the selection of cases for this modality, and further study must be devoted to develop diagnostic guidelines of ever-increasing precision to determine which breasts are potentially malignant and should be afforded the procedure."}
{"id": "PMID:1097167", "title": "Ultrasound in the diagnosis of fluid collections following renal transplantation.", "content": "Ultrasound examinations of the pelvis and kidney have been carried out following 68 renal transplants. In 16 patients moderate sized or large perirenal or pelvic fluid collections were demonstrated. The composition of the fluid was lymph in seven cases, pus in four, blood in three and urine in two. Ten of these 16 patients had clinical features compatible with a pelvic fulid collection while six had no such localising features. We have found ultrasound to be extremely valuable in the diagnosis and precise localisation of pelvic fulid collections following transplantation, often at a stage before pressure effects and symptoms have appeared. It also provides a rapid and reliable means of excluding a pelvic or perirenal abscess in the pyrexial patient.", "contents": "Ultrasound in the diagnosis of fluid collections following renal transplantation. Ultrasound examinations of the pelvis and kidney have been carried out following 68 renal transplants. In 16 patients moderate sized or large perirenal or pelvic fluid collections were demonstrated. The composition of the fluid was lymph in seven cases, pus in four, blood in three and urine in two. Ten of these 16 patients had clinical features compatible with a pelvic fulid collection while six had no such localising features. We have found ultrasound to be extremely valuable in the diagnosis and precise localisation of pelvic fulid collections following transplantation, often at a stage before pressure effects and symptoms have appeared. It also provides a rapid and reliable means of excluding a pelvic or perirenal abscess in the pyrexial patient."}
{"id": "PMID:1097171", "title": "Effect of proper oral hygiene on gingivitis and dental caries in Swedish schoolchildren.", "content": "The present paper reports the effect of a 3-year study of regularly repeated professional toothcleaning on oral hygiene status, gingivitis, and dental caries. At the initiation of the study in 1971, the children were 7-8 years old (Group 1), 10-11 (Group 2) or 13-14 (Group 3). The children were divided into test and control groups. Before the study all the children were examined regarding oral hygiene, state of the gingiva and dental caries. Throughout the 3-year period the controls brushed their teeth with a 0.2% sodium fluoride solution once a month under supervision. During the first 2 years of the trial all the children of the test groups were given professional oral prophylaxis once every second week during the school terms. During the third year the interval between consecutive prophylactic sessions was prolonged to 4 weeks in Groups 1 and 2 and to 8 weeks in Group 3. The results demonstrated that regularly repeated professional cleaning of the teeth combined with fluoride applications and toothbrushing instructions, over a 3-year period in schoolchildren resulted in the establishment and maintenance of excellent oral hygiene standards. Inflammation of the marginal gingiva almost entirely disappeared, and practically no new carious lesions developed.", "contents": "Effect of proper oral hygiene on gingivitis and dental caries in Swedish schoolchildren. The present paper reports the effect of a 3-year study of regularly repeated professional toothcleaning on oral hygiene status, gingivitis, and dental caries. At the initiation of the study in 1971, the children were 7-8 years old (Group 1), 10-11 (Group 2) or 13-14 (Group 3). The children were divided into test and control groups. Before the study all the children were examined regarding oral hygiene, state of the gingiva and dental caries. Throughout the 3-year period the controls brushed their teeth with a 0.2% sodium fluoride solution once a month under supervision. During the first 2 years of the trial all the children of the test groups were given professional oral prophylaxis once every second week during the school terms. During the third year the interval between consecutive prophylactic sessions was prolonged to 4 weeks in Groups 1 and 2 and to 8 weeks in Group 3. The results demonstrated that regularly repeated professional cleaning of the teeth combined with fluoride applications and toothbrushing instructions, over a 3-year period in schoolchildren resulted in the establishment and maintenance of excellent oral hygiene standards. Inflammation of the marginal gingiva almost entirely disappeared, and practically no new carious lesions developed."}
{"id": "PMID:1097172", "title": "The psychiatric halfway house: how is it measuring up?", "content": "To obtain data on the effectiveness of the psychiatric halfway house and to update information about such facilities, 26 recent statistical reports were examined. Only two studies include control group comparisons. In addition to descriptive information, data are reported in terms of employment or school attendance and independent living as contrasted with rehospitalization. Results indicate that a median of approximately 80 per cent of halfway house residents adjusts to community living. Despite differences among halfway houses in resident selection, length of stay, rehabilitative criteria, and nature of follow-up, data suggest that rehospitalization rates are lower after residence in a halfway house. Some cautions are drawn regarding evaluative studies and potential attenuation of halfway house facilities to low-quality custodial care.", "contents": "The psychiatric halfway house: how is it measuring up? To obtain data on the effectiveness of the psychiatric halfway house and to update information about such facilities, 26 recent statistical reports were examined. Only two studies include control group comparisons. In addition to descriptive information, data are reported in terms of employment or school attendance and independent living as contrasted with rehospitalization. Results indicate that a median of approximately 80 per cent of halfway house residents adjusts to community living. Despite differences among halfway houses in resident selection, length of stay, rehabilitative criteria, and nature of follow-up, data suggest that rehospitalization rates are lower after residence in a halfway house. Some cautions are drawn regarding evaluative studies and potential attenuation of halfway house facilities to low-quality custodial care."}
{"id": "PMID:1097173", "title": "The relationship between patient demographic characteristics and psychiatric rehabilitation outcome.", "content": "Professionals engaged in psychiatric rehabilitation need to know what specific factors contribute to the success or failure of attempts to reintegrate the psychiatric patient back into the community. A review of the research literature succeeded in identifying various demographic charcteristics of expsychiatric patients that are related to recidivism and posthospital employment. A composite picture of patients who are more likely to be recidivists or unemployed (or both) was advanced. The factors that appear to be related to posthospital employment are not consistently the same factors that are related to recidivism.", "contents": "The relationship between patient demographic characteristics and psychiatric rehabilitation outcome. Professionals engaged in psychiatric rehabilitation need to know what specific factors contribute to the success or failure of attempts to reintegrate the psychiatric patient back into the community. A review of the research literature succeeded in identifying various demographic charcteristics of expsychiatric patients that are related to recidivism and posthospital employment. A composite picture of patients who are more likely to be recidivists or unemployed (or both) was advanced. The factors that appear to be related to posthospital employment are not consistently the same factors that are related to recidivism."}
{"id": "PMID:1097175", "title": "Stereotactic treatment of action myoclonus in a case of combined status marmoratus and multiple sclerosis. A contribution to the pathophysiology of basal ganglia with multiple lesions in both the striatum and the substantia nigra.", "content": "A patent, who suffered from nonprogressive athetotic-myoclonic hyperkinesia of the left arm and spastic paresis of the underdeveloped left leg since early childhood, experienced a febrile episode at the age of 23, after which a weakness and ataxia of the right limbs with frequent falling persisted; at the age of 28, he developed a right-sided rigidity, tremor at rest and mask-like face as sequelae of encephalitis disseminata exacerbations. The violent intention myoclonus of the left side of the body could be abolished by stereotactic coagulation in the V.o.p and V.o.a and zona incerta until death 11 days later. The athetotic myoclonic hyperkinesia is the consequence of a left-sided severe status marmoratus of the right putamen with extensive loss of nerve cells and shrinkage. The additional nerve cell loss in the right substantia nigra due to demyelinating encephalitic foci did not produce Parkinson symptoms, because these require a normal striatum. This loss did, however, exaggerate the original hyperkinesia to a violent intention myoclonus, which was abolished by interruption of denatatothalamic afferents to the V.o.p nucleus and of pallidothalamic afferents to the V.o.a nucleus. The Parkinson syndrome of the right side is due to demyelinating foci of different ages. The Parkinson symptoms were manifest on the right side, because the left status marmoratus did not severely reduce the striatal nerve cells. In this case, there is no indication that the introduction of the stereotactic electrode has precipitated new demyelinating foci.", "contents": "Stereotactic treatment of action myoclonus in a case of combined status marmoratus and multiple sclerosis. A contribution to the pathophysiology of basal ganglia with multiple lesions in both the striatum and the substantia nigra. A patent, who suffered from nonprogressive athetotic-myoclonic hyperkinesia of the left arm and spastic paresis of the underdeveloped left leg since early childhood, experienced a febrile episode at the age of 23, after which a weakness and ataxia of the right limbs with frequent falling persisted; at the age of 28, he developed a right-sided rigidity, tremor at rest and mask-like face as sequelae of encephalitis disseminata exacerbations. The violent intention myoclonus of the left side of the body could be abolished by stereotactic coagulation in the V.o.p and V.o.a and zona incerta until death 11 days later. The athetotic myoclonic hyperkinesia is the consequence of a left-sided severe status marmoratus of the right putamen with extensive loss of nerve cells and shrinkage. The additional nerve cell loss in the right substantia nigra due to demyelinating encephalitic foci did not produce Parkinson symptoms, because these require a normal striatum. This loss did, however, exaggerate the original hyperkinesia to a violent intention myoclonus, which was abolished by interruption of denatatothalamic afferents to the V.o.p nucleus and of pallidothalamic afferents to the V.o.a nucleus. The Parkinson syndrome of the right side is due to demyelinating foci of different ages. The Parkinson symptoms were manifest on the right side, because the left status marmoratus did not severely reduce the striatal nerve cells. In this case, there is no indication that the introduction of the stereotactic electrode has precipitated new demyelinating foci."}
{"id": "PMID:1097185", "title": "Nuclear placentography.", "content": "Localization of the placenta by radionuclide imaging techniques is useful in assessing the cause of vaginal bleeding late in pregnancy. Placenta previa is the most threatening cause of late gestational bleeding, and the acceptably high accuracy of nuclear placentography in detecting this condition is well-established. Nevertheless, B-mode ultrasonography is a preferable method because it delivers no ionizing radiation and is capable of identifying the cervix in virtually all patients. The latter method will eventually replace nuclear placentography.", "contents": "Nuclear placentography. Localization of the placenta by radionuclide imaging techniques is useful in assessing the cause of vaginal bleeding late in pregnancy. Placenta previa is the most threatening cause of late gestational bleeding, and the acceptably high accuracy of nuclear placentography in detecting this condition is well-established. Nevertheless, B-mode ultrasonography is a preferable method because it delivers no ionizing radiation and is capable of identifying the cervix in virtually all patients. The latter method will eventually replace nuclear placentography."}
{"id": "PMID:1097186", "title": "Evaluation of intracardiac shunts.", "content": "This article will review the qualitative and quantitative methods of radionuclide evaluation of intracardiac shunts. Radionuclide angiocardiography of the cardiopulmonary system has proven adequate, with few exceptions, for qualitative detection of left-to-right and right-to-left intracardiac shunts. Numerous authors have reported on the detection of intracardiac shunts using qualitative methods such as rapid sequential visualization of nuclide angiocardiography using the scintillation camera with Polaroid, 35mm or 70mm film. Recent development of videotape storage systems and computers offers a quantitative technique of permanently recording the nuclide angiocardiogram and thus the capability for replay and analysis of the study. Quantitative analysis of the nuclide angiogram in the form of heart chamber dilution curves or pulmonary dilution curves permits 1) determination of the presence or absence of left-to-right, right-to-left, or bi-directional intracardiac shunts, including shunts which might go undetected using the qualitative techniques, 2) shunt location at the atrial or ventricular level, 3) determination of the functional status of a corrective left-to-right shunt, and 4) shunt quantitation. By quantitative analysis of the isotope dilution curves, it is possible to calculate the size of left-to-right or right-to-left intracardiac shunts (Qp/Qs ratio) to within 10% of the values obtained at cardiac catheterization.", "contents": "Evaluation of intracardiac shunts. This article will review the qualitative and quantitative methods of radionuclide evaluation of intracardiac shunts. Radionuclide angiocardiography of the cardiopulmonary system has proven adequate, with few exceptions, for qualitative detection of left-to-right and right-to-left intracardiac shunts. Numerous authors have reported on the detection of intracardiac shunts using qualitative methods such as rapid sequential visualization of nuclide angiocardiography using the scintillation camera with Polaroid, 35mm or 70mm film. Recent development of videotape storage systems and computers offers a quantitative technique of permanently recording the nuclide angiocardiogram and thus the capability for replay and analysis of the study. Quantitative analysis of the nuclide angiogram in the form of heart chamber dilution curves or pulmonary dilution curves permits 1) determination of the presence or absence of left-to-right, right-to-left, or bi-directional intracardiac shunts, including shunts which might go undetected using the qualitative techniques, 2) shunt location at the atrial or ventricular level, 3) determination of the functional status of a corrective left-to-right shunt, and 4) shunt quantitation. By quantitative analysis of the isotope dilution curves, it is possible to calculate the size of left-to-right or right-to-left intracardiac shunts (Qp/Qs ratio) to within 10% of the values obtained at cardiac catheterization."}
{"id": "PMID:1097187", "title": "The significance of soft tissue injury in the roentgen diagnosis of trauma.", "content": "Soft tissue damage is an integral part of trauma. This basic fact is commonly ignored in the evaluation of roentgenograms obtained because of trauma. The soft tissue component of the traumatic lesion may produce the most obvious roentgen sing of injury and is, in these instances, fundamental to the correct roentgen diagnosis. In many instances, the soft tissue lesion is frequently of greater clinical significance that the more obvious skeletal injury. An awareness of the roentgen signs that signal soft tissue injury, and of those skeletal lesions that are commonly associated with radiographically silent, but clinically more significant, soft tissue damage, leads to prompt institution of appropriate additional diagnostic studies or therapeutic measures. Thus, the soft tissue injury in the roentgen diagnosis of trauma has both diagnostic and therapeutic significance. Each will be discussed in this article.", "contents": "The significance of soft tissue injury in the roentgen diagnosis of trauma. Soft tissue damage is an integral part of trauma. This basic fact is commonly ignored in the evaluation of roentgenograms obtained because of trauma. The soft tissue component of the traumatic lesion may produce the most obvious roentgen sing of injury and is, in these instances, fundamental to the correct roentgen diagnosis. In many instances, the soft tissue lesion is frequently of greater clinical significance that the more obvious skeletal injury. An awareness of the roentgen signs that signal soft tissue injury, and of those skeletal lesions that are commonly associated with radiographically silent, but clinically more significant, soft tissue damage, leads to prompt institution of appropriate additional diagnostic studies or therapeutic measures. Thus, the soft tissue injury in the roentgen diagnosis of trauma has both diagnostic and therapeutic significance. Each will be discussed in this article."}
{"id": "PMID:1097188", "title": "Peripheral arteriography an overview of its origins and present status.", "content": "Within a month after Roentgen's discovery of the X-ray, the first cadaveric peripheral arteriogram was performed. It was not until 28 years later that the initial in vivo femoral arteriogram was reported. Arteriographic growth and development since that time have been punctuated with such names as Seldinger, Steinberg, Viamonte, Abrams, Sones, and many more. Keeping pace with their technical and diagnostic achievements has been the cause for a whole new industrial field - that of angiographic equipment and technology. New X-ray tubes, generators, film, etc. have been developed. Angiographic tables are available which have been designed to do highly subspecialized studies. The spectrum of catheter curves and materials available allows visualization of vessels down to the order of 0.1 mm when combined with magnification. Chemoangiography for treatment and subselective venous sampling for hormonal assays are commonplace. Although \"the beginnings\" for all angiography lie in the evaluation of peripheral vasculature, equipment sophistication for this basic study has often lagged behind that of the more glamorous organal examinations. The development of the biphasic ijection technique hopefully represents an anwer to both multiple injections and X-ray exposures. It allows single injection and single exposure visualization of the vascular tree from distal aorta to the pedal vessels.", "contents": "Peripheral arteriography an overview of its origins and present status. Within a month after Roentgen's discovery of the X-ray, the first cadaveric peripheral arteriogram was performed. It was not until 28 years later that the initial in vivo femoral arteriogram was reported. Arteriographic growth and development since that time have been punctuated with such names as Seldinger, Steinberg, Viamonte, Abrams, Sones, and many more. Keeping pace with their technical and diagnostic achievements has been the cause for a whole new industrial field - that of angiographic equipment and technology. New X-ray tubes, generators, film, etc. have been developed. Angiographic tables are available which have been designed to do highly subspecialized studies. The spectrum of catheter curves and materials available allows visualization of vessels down to the order of 0.1 mm when combined with magnification. Chemoangiography for treatment and subselective venous sampling for hormonal assays are commonplace. Although \"the beginnings\" for all angiography lie in the evaluation of peripheral vasculature, equipment sophistication for this basic study has often lagged behind that of the more glamorous organal examinations. The development of the biphasic ijection technique hopefully represents an anwer to both multiple injections and X-ray exposures. It allows single injection and single exposure visualization of the vascular tree from distal aorta to the pedal vessels."}
{"id": "PMID:1097189", "title": "A survey of the benefits and risks in the practice of radiology.", "content": "The findings from both animal and human studies on the radiation risk at low doses and low dose rates are far from conclusive, primarily due to statistical limitations. However, to arrive at some estimate of radiation risk, a conservative approach has been taken, and a linear extrapolation of radiation effects from high doses to low doses has been made. Thus, it is assumed that any exposure to radiation carries some risk of somatic or genetic damage and that there is no threshold or safe dose. In medical practice, diagnostic radiological procedures should be performed only if useful clinical information will be derived. In addition, this information should be obtained at the least possible risk to the patient. For mass chest X-rays, mammography, and lung scans, a quantitative determination of benefit to risk is developed. This approach, though possible desirable, is exceedingly difficult to establish for most diagnostic radiological procedures. Thus, good clinical judgement should be employed in radiological practice, just as it should be employed in all aspects of medical practice.", "contents": "A survey of the benefits and risks in the practice of radiology. The findings from both animal and human studies on the radiation risk at low doses and low dose rates are far from conclusive, primarily due to statistical limitations. However, to arrive at some estimate of radiation risk, a conservative approach has been taken, and a linear extrapolation of radiation effects from high doses to low doses has been made. Thus, it is assumed that any exposure to radiation carries some risk of somatic or genetic damage and that there is no threshold or safe dose. In medical practice, diagnostic radiological procedures should be performed only if useful clinical information will be derived. In addition, this information should be obtained at the least possible risk to the patient. For mass chest X-rays, mammography, and lung scans, a quantitative determination of benefit to risk is developed. This approach, though possible desirable, is exceedingly difficult to establish for most diagnostic radiological procedures. Thus, good clinical judgement should be employed in radiological practice, just as it should be employed in all aspects of medical practice."}
{"id": "PMID:1097194", "title": "A comparative study of the activity of a new agent, indapamide, in essential arterial hypertension.", "content": "A single-blind crossover trial was carried out in 38 elderly, hospitalised patients with essential hypertension to compare the hypotensive activity of 5 mg. indapamide daily with 100 mg. chlorthalidone daily. After initial treatment for 10 days with placebo, patients received treatment for 45 days with either indapamide or chlorthalidone and were then crossed over to the alternative drug for a similar period. Potassium supplementation was necessary in 25 of the patients receiving chlorthalidone, but was precribed as a precautionary measure in only 1 patients whilst on indapamide. Results showed that there were significant drops in blood pressure following both active medications, but that the percentage reduction in diastolic pressure was greater after indapamide. Indapamide also proved more effective than chlorthalidone in controlling the patients' subjective and functional symptoms of their hypertension. In an overall assessment of the effectiveness of both drugs, indapamide was judged to be better tolerated as well as more effective than chlorthalidone in 18 of the 38 patients, whilst chlorthalidone was preferred in only 7 instances.", "contents": "A comparative study of the activity of a new agent, indapamide, in essential arterial hypertension. A single-blind crossover trial was carried out in 38 elderly, hospitalised patients with essential hypertension to compare the hypotensive activity of 5 mg. indapamide daily with 100 mg. chlorthalidone daily. After initial treatment for 10 days with placebo, patients received treatment for 45 days with either indapamide or chlorthalidone and were then crossed over to the alternative drug for a similar period. Potassium supplementation was necessary in 25 of the patients receiving chlorthalidone, but was precribed as a precautionary measure in only 1 patients whilst on indapamide. Results showed that there were significant drops in blood pressure following both active medications, but that the percentage reduction in diastolic pressure was greater after indapamide. Indapamide also proved more effective than chlorthalidone in controlling the patients' subjective and functional symptoms of their hypertension. In an overall assessment of the effectiveness of both drugs, indapamide was judged to be better tolerated as well as more effective than chlorthalidone in 18 of the 38 patients, whilst chlorthalidone was preferred in only 7 instances."}
{"id": "PMID:1097195", "title": "Preliminary clinical experience with meptazinol, a new analgesic.", "content": "The analgesic effect of intravenous meptazinol at various dosage levels was studied in 30 patients suffering from acute renal colic. Out of a total of 41 injections (dose range: 20 to 120 mg.) a favourable analgesic response was recorded on 39 occasions. The onset of action was rapid with the minimun duration of action of 1 hour. Higher doses increased the drug's action but were accompanied by nausea and dizziness. There was no overall effect on blood pressure or heart rate.", "contents": "Preliminary clinical experience with meptazinol, a new analgesic. The analgesic effect of intravenous meptazinol at various dosage levels was studied in 30 patients suffering from acute renal colic. Out of a total of 41 injections (dose range: 20 to 120 mg.) a favourable analgesic response was recorded on 39 occasions. The onset of action was rapid with the minimun duration of action of 1 hour. Higher doses increased the drug's action but were accompanied by nausea and dizziness. There was no overall effect on blood pressure or heart rate."}
{"id": "PMID:1097196", "title": "Cephradine in urinary tract infections: a double-blind comparison with ampicillin.", "content": "A double-blind trial was carried out comparing cephradine and ampicillin in the treatment of urinary tract infections in 42 females attending a hospital Department of Obstetrics and Gynaecology. Results showed that patients did better in the cephradine group than the ampicillin group, both bacteriologically and clinically. The numbers in each group were small, however, and did not reach statistical significance.", "contents": "Cephradine in urinary tract infections: a double-blind comparison with ampicillin. A double-blind trial was carried out comparing cephradine and ampicillin in the treatment of urinary tract infections in 42 females attending a hospital Department of Obstetrics and Gynaecology. Results showed that patients did better in the cephradine group than the ampicillin group, both bacteriologically and clinically. The numbers in each group were small, however, and did not reach statistical significance."}
{"id": "PMID:1097197", "title": "Cyclamates: a review of the current position.", "content": "Cyclamates were prohibited for use as food additives in the U.S.A. and other parts of the world during 1970 because of the fears of carcinogenicity. The author reviews the evidence leading to this ban and discusses the appeal made against the decision which led to the lifting of the cyclamate restrictions in Australia in November 1974.", "contents": "Cyclamates: a review of the current position. Cyclamates were prohibited for use as food additives in the U.S.A. and other parts of the world during 1970 because of the fears of carcinogenicity. The author reviews the evidence leading to this ban and discusses the appeal made against the decision which led to the lifting of the cyclamate restrictions in Australia in November 1974."}
{"id": "PMID:1097198", "title": "Double-blind comparison between two new topical corticosteroids, halcinonide 0.1% and clobetasol propionate cream 0.05%.", "content": "A new steroid, halcinonide, was compared with clobetasol propionate cream in 100 patients with a variety of skin disorders. The study was conducted as a double-blind, paired comparison with the preparations being given twice daily. Results obtained over a 14-day period of therapy showed remarkably similar efficacy for both preparations.", "contents": "Double-blind comparison between two new topical corticosteroids, halcinonide 0.1% and clobetasol propionate cream 0.05%. A new steroid, halcinonide, was compared with clobetasol propionate cream in 100 patients with a variety of skin disorders. The study was conducted as a double-blind, paired comparison with the preparations being given twice daily. Results obtained over a 14-day period of therapy showed remarkably similar efficacy for both preparations."}
{"id": "PMID:1097199", "title": "Nucleic acids: interaction with solar UV radiation.", "content": "Atmospheric pollutants that reduce the amount of ozone in the stratosphere may markedly increase the flux of intermediate-wavelength solar ultraviolet (UV) radiation that reaches the Earth's surface. Like short-wavelength germicidal UV radiation (less than 280 nm), these intermediate UV wavelengths (280-315 nm) can promote photochemical reactions in nucleic acids, leading to the appearance of such products as cyclobutadipyrimidines and single- and double-strand breaks. These photochemical reactions strongly affect the biological activities of the nucleic acids. Computer techniques are now available for predicting the chemical and biological effects of increased in vitro irradiation of purified nucleic acids. However, the effect of increased UV irradiation in vivo is complicated by the presence of sensitizing agents in cells and by the action of nucleic acid repair processes. There is strong evidence that in vivo damage to nucleic acids injures irradiated cells and tissues, but further research is needed to predict quantitatively the physiological consequences of increases in solar UV.", "contents": "Nucleic acids: interaction with solar UV radiation. Atmospheric pollutants that reduce the amount of ozone in the stratosphere may markedly increase the flux of intermediate-wavelength solar ultraviolet (UV) radiation that reaches the Earth's surface. Like short-wavelength germicidal UV radiation (less than 280 nm), these intermediate UV wavelengths (280-315 nm) can promote photochemical reactions in nucleic acids, leading to the appearance of such products as cyclobutadipyrimidines and single- and double-strand breaks. These photochemical reactions strongly affect the biological activities of the nucleic acids. Computer techniques are now available for predicting the chemical and biological effects of increased in vitro irradiation of purified nucleic acids. However, the effect of increased UV irradiation in vivo is complicated by the presence of sensitizing agents in cells and by the action of nucleic acid repair processes. There is strong evidence that in vivo damage to nucleic acids injures irradiated cells and tissues, but further research is needed to predict quantitatively the physiological consequences of increases in solar UV."}
{"id": "PMID:1097202", "title": "Combined topical pulmonary and systemic gentamicin: The question of safety.", "content": "Serum levels were measured in 13 subjects who received gentamicin either by inspiratory intermittent positive pressure breathing (IPPB-I) or by tracheal instillation. Only in the latter group were significant serum levels achieved. It is recommended that systemic doses of gentamicin should be reduced and serum levels closely monitored in order to avoid toxicity when the usual 3 to 5 mg/kg of IM or IV gentamicin is conbined with tracheal instillation.", "contents": "Combined topical pulmonary and systemic gentamicin: The question of safety. Serum levels were measured in 13 subjects who received gentamicin either by inspiratory intermittent positive pressure breathing (IPPB-I) or by tracheal instillation. Only in the latter group were significant serum levels achieved. It is recommended that systemic doses of gentamicin should be reduced and serum levels closely monitored in order to avoid toxicity when the usual 3 to 5 mg/kg of IM or IV gentamicin is conbined with tracheal instillation."}
{"id": "PMID:1097203", "title": "Double-blind comparison of acute bronchial and cardiovascular effects of oral terbutaline and ephedrine.", "content": "Acute effects of oral terbutaline (5 mg), ephedrine (25 mg) and placebo on bronchial dynamics, heart rate, systolic and diastolic pressure and arterial blood gases at rest were compared over a period of 7 hours in 20 subjects with bronchial asthma using a double-blind crossover technique. Both terbutaline and ephedrine caused significant bronchodilation, but the effect of terbutaline on specific airway conductance (SGA) was significantly greater (peak mean increase in SGA 0.069 vs 0.027 L/sec/cm H2O/L), had an earlier onset (30 minutes vs 1 hour) and lasted longer (7 hours vs 4 hours) than that of ephedrine. Slight but significant increases in arterial Po2 were noted following institution of both ephedrine and terbutaline, suggesting improvement in ventilation-perfusion relationships. Both drugs caused modest but statistically significant increases in heart rate of 8.4-10.9 beats/min and the mean peak increase following terbutaline (8.4 beats/min) was comparable to that following ephedrine. In constrast to ephedrine, terbutaline caused significant, although slight, increases in systolic pressure and decreases in diastolic pressure, indicating that this drug, in the dose recommended for clinical use, is not free of cardiovascular effects.", "contents": "Double-blind comparison of acute bronchial and cardiovascular effects of oral terbutaline and ephedrine. Acute effects of oral terbutaline (5 mg), ephedrine (25 mg) and placebo on bronchial dynamics, heart rate, systolic and diastolic pressure and arterial blood gases at rest were compared over a period of 7 hours in 20 subjects with bronchial asthma using a double-blind crossover technique. Both terbutaline and ephedrine caused significant bronchodilation, but the effect of terbutaline on specific airway conductance (SGA) was significantly greater (peak mean increase in SGA 0.069 vs 0.027 L/sec/cm H2O/L), had an earlier onset (30 minutes vs 1 hour) and lasted longer (7 hours vs 4 hours) than that of ephedrine. Slight but significant increases in arterial Po2 were noted following institution of both ephedrine and terbutaline, suggesting improvement in ventilation-perfusion relationships. Both drugs caused modest but statistically significant increases in heart rate of 8.4-10.9 beats/min and the mean peak increase following terbutaline (8.4 beats/min) was comparable to that following ephedrine. In constrast to ephedrine, terbutaline caused significant, although slight, increases in systolic pressure and decreases in diastolic pressure, indicating that this drug, in the dose recommended for clinical use, is not free of cardiovascular effects."}
{"id": "PMID:1097204", "title": "A computer program for calculation and interpretation of pulmonary function studies.", "content": "A computer program to calculate and interpret the standard pulmonary function tests has been developed on a programmable calculator system. The program computes both predicted and measured values for static and dynamic lung volumes, airways resistance and diffusing capacity. It then checks for differences between predicted and obtained values and interprets them according to a specific, clinically useful set of diagnostic criteria. By acquisition of a reliable and easy-to-use data processing system, a pulmonary function laboratory can significantly increase the efficiency and accuracy of its day-to-day work.", "contents": "A computer program for calculation and interpretation of pulmonary function studies. A computer program to calculate and interpret the standard pulmonary function tests has been developed on a programmable calculator system. The program computes both predicted and measured values for static and dynamic lung volumes, airways resistance and diffusing capacity. It then checks for differences between predicted and obtained values and interprets them according to a specific, clinically useful set of diagnostic criteria. By acquisition of a reliable and easy-to-use data processing system, a pulmonary function laboratory can significantly increase the efficiency and accuracy of its day-to-day work."}
{"id": "PMID:1097206", "title": "In vivo comparison of two galenic forms of ampicillin-trihydrate.", "content": "In order to evaluate a new galenic form of ampicillin-trihydrate, individual blood serum levels and urine excretion of ampicillin-trihydrate were compared in a crossover study after oral absorption of 1-gram captab formulation (this being the new galenic form) versus 2x500 mg of the same product. Analyses performed by two different laboratories indicate that clinically insignificant slightly higher serum concentrations can be obtained with captabs, and that very individual absorption profiles should make one take a sceptic viewpoint as to mean serum concentrations computed from larger series of experiments. To obtain a valid blood serum level in as many patients as possible, a posology of 3-4 captabs, spaced over a 24-hour period is suggested for the treatment of infections with ampicillin-sensitive germs. For less sensitive micro-organisms, one should switch to parenteral therapy or to other antibiotics.", "contents": "In vivo comparison of two galenic forms of ampicillin-trihydrate. In order to evaluate a new galenic form of ampicillin-trihydrate, individual blood serum levels and urine excretion of ampicillin-trihydrate were compared in a crossover study after oral absorption of 1-gram captab formulation (this being the new galenic form) versus 2x500 mg of the same product. Analyses performed by two different laboratories indicate that clinically insignificant slightly higher serum concentrations can be obtained with captabs, and that very individual absorption profiles should make one take a sceptic viewpoint as to mean serum concentrations computed from larger series of experiments. To obtain a valid blood serum level in as many patients as possible, a posology of 3-4 captabs, spaced over a 24-hour period is suggested for the treatment of infections with ampicillin-sensitive germs. For less sensitive micro-organisms, one should switch to parenteral therapy or to other antibiotics."}
{"id": "PMID:1097207", "title": "Comparative pharmacokinetics and clinical experience with a new cephalosporin-derivative: cefazolin.", "content": "In a cross-over study in 12 normal individuals, the pharmacokinetic parameters of cefalotin, cefradine and cefazolin were determined after intravenous injection of 1,000 mg of each substance. The microbiological activities in urine and serum were determined using the agar diffusion test; the pharmacokinetic data were calculated by a computer system on the basis of a Fortran programme. Cefazolin has significantly higher serum concentrations than the other two cephalosporins, distinctly longer serum half-lives, higher protein binding, and smaller apparent volumina of distribution. In 36 inpatients with mainly chronical and acute infections of the urinary tract, we tested the antibacterial effectivity, the compatibility, and the application modalities of cefazolin. In 29 patients we had a satisfactory clinical result, in 25 cases we achieved the elimination of bacteria by the end of the therapy. The compatibility of cefazolin was good; apart from a minor, reversible, liver-specific increase in enzymes in 6 patients, no side effects could be detected.", "contents": "Comparative pharmacokinetics and clinical experience with a new cephalosporin-derivative: cefazolin. In a cross-over study in 12 normal individuals, the pharmacokinetic parameters of cefalotin, cefradine and cefazolin were determined after intravenous injection of 1,000 mg of each substance. The microbiological activities in urine and serum were determined using the agar diffusion test; the pharmacokinetic data were calculated by a computer system on the basis of a Fortran programme. Cefazolin has significantly higher serum concentrations than the other two cephalosporins, distinctly longer serum half-lives, higher protein binding, and smaller apparent volumina of distribution. In 36 inpatients with mainly chronical and acute infections of the urinary tract, we tested the antibacterial effectivity, the compatibility, and the application modalities of cefazolin. In 29 patients we had a satisfactory clinical result, in 25 cases we achieved the elimination of bacteria by the end of the therapy. The compatibility of cefazolin was good; apart from a minor, reversible, liver-specific increase in enzymes in 6 patients, no side effects could be detected."}
{"id": "PMID:1097208", "title": "R factor in Enterobacter cloacae resistant to silver sulfadiazine.", "content": "A strain of Enterobacter cloacae resistant to silver sulfadiazine was recovered from a unit in which this antimicrobial agent was in use. This strain was found to harbor an R factor responsible for resistance to carbenicillin, kanamycin and ampicillin. These antibiotic resistances were transferable to Escherichia coli by mating. Resistance to silver sulfadiazine was not, however, transmissible.", "contents": "R factor in Enterobacter cloacae resistant to silver sulfadiazine. A strain of Enterobacter cloacae resistant to silver sulfadiazine was recovered from a unit in which this antimicrobial agent was in use. This strain was found to harbor an R factor responsible for resistance to carbenicillin, kanamycin and ampicillin. These antibiotic resistances were transferable to Escherichia coli by mating. Resistance to silver sulfadiazine was not, however, transmissible."}
{"id": "PMID:1097209", "title": "Comparative in vitro acitvity of chloramphenicol and thiamphenicol on common aerobic and anaerobic gram-negative bacilli(Salmonella and Shigella excluded).", "content": "The antibacterial activity of thiamphenicol was compared to that of chloramphenicol against 313 strains of gram-negative bacilli isolated from various clinical specimens. These two antiboitics were equally active against the 106 isolates of Haemophilus MIC equals 0.1 minus 1.56 mu g/ml) and against 40 strains of Bacteroides fragilis (almost all strains being inhibited by 12.5 mug/ml of the two drugs). In contrast, when compared with chloramphenicol, 2-16 times as much of thiamphenicol was required to inhibit Enterobacteriacae, making prediction of the susceptibility of these strains to thiamphenicol on the basis of chloramphenicol testing alone likely to be hazardous. Disc diffusion test using 30-mug discs and 12 mm as cut-off point was a reliable technique to determine susceptibility of bacteria either to chloramphenicol or thiamphenicol. When thiamphenicol discs of greater potency (50 mug) were employed, many strains exhibited wide zones of inhibition although most of them were resistant by the agar dilution method (MIC is greater than 12.5 mug/ml). This practice is not advisable for testing organisms isolated outside of the urinary tract.", "contents": "Comparative in vitro acitvity of chloramphenicol and thiamphenicol on common aerobic and anaerobic gram-negative bacilli(Salmonella and Shigella excluded). The antibacterial activity of thiamphenicol was compared to that of chloramphenicol against 313 strains of gram-negative bacilli isolated from various clinical specimens. These two antiboitics were equally active against the 106 isolates of Haemophilus MIC equals 0.1 minus 1.56 mu g/ml) and against 40 strains of Bacteroides fragilis (almost all strains being inhibited by 12.5 mug/ml of the two drugs). In contrast, when compared with chloramphenicol, 2-16 times as much of thiamphenicol was required to inhibit Enterobacteriacae, making prediction of the susceptibility of these strains to thiamphenicol on the basis of chloramphenicol testing alone likely to be hazardous. Disc diffusion test using 30-mug discs and 12 mm as cut-off point was a reliable technique to determine susceptibility of bacteria either to chloramphenicol or thiamphenicol. When thiamphenicol discs of greater potency (50 mug) were employed, many strains exhibited wide zones of inhibition although most of them were resistant by the agar dilution method (MIC is greater than 12.5 mug/ml). This practice is not advisable for testing organisms isolated outside of the urinary tract."}
{"id": "PMID:1097210", "title": "Evaluation of antibacterial activities of cephalosporin antibiotics: cefazolin, cephaloridine, cephalothin, and cephalexin.", "content": "The antibacterial activities of 4 cephalosporin antibiotics, cefazolin, cephaloridine, cephalothin and cephalexin, against 330 isolates of bacteria from patients at National Taiwan University Hospital, were determined by an agar plate dilution technique. Cephalosporins possess strong antibacterial activity against gram-positive bacteria except Enterococci. Staphylococcus aureus is the most susceptible among the organisms tested. More than 90% of Staphylococcus aureus strains are suppressed by cefazolin, cephaloridine and cephalothin at the concentrations of 3.13 mug/ml or less, except that 49.1% are suppressed by cephalexin. The relative potency of cephalosporins against Staphylococcus aureus in decreasing order is cephaloridine, cephalothin, cefazolin, and cephalexin. The gram-negative bacilli, Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis are less susceptible to cephalosporins than the gram-positive cocci. Among the cephalosporins, cefazolin is the most active against the gram-negative pathogens tested. The relative potency of antibacterial activity of cephalosporins against E. coli in decreasing order is cefazolin, cephaloridine, cephalexin, and cephalothin. One hour after the intramuscular injection of 500 mg of cefazolin, the maximum concentration of 33.3 mug/ml is reached in the serum. The sufficient high levels are sustained for 8 hours. Very high concentrations of cefazolin are also found in the urine.", "contents": "Evaluation of antibacterial activities of cephalosporin antibiotics: cefazolin, cephaloridine, cephalothin, and cephalexin. The antibacterial activities of 4 cephalosporin antibiotics, cefazolin, cephaloridine, cephalothin and cephalexin, against 330 isolates of bacteria from patients at National Taiwan University Hospital, were determined by an agar plate dilution technique. Cephalosporins possess strong antibacterial activity against gram-positive bacteria except Enterococci. Staphylococcus aureus is the most susceptible among the organisms tested. More than 90% of Staphylococcus aureus strains are suppressed by cefazolin, cephaloridine and cephalothin at the concentrations of 3.13 mug/ml or less, except that 49.1% are suppressed by cephalexin. The relative potency of cephalosporins against Staphylococcus aureus in decreasing order is cephaloridine, cephalothin, cefazolin, and cephalexin. The gram-negative bacilli, Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis are less susceptible to cephalosporins than the gram-positive cocci. Among the cephalosporins, cefazolin is the most active against the gram-negative pathogens tested. The relative potency of antibacterial activity of cephalosporins against E. coli in decreasing order is cefazolin, cephaloridine, cephalexin, and cephalothin. One hour after the intramuscular injection of 500 mg of cefazolin, the maximum concentration of 33.3 mug/ml is reached in the serum. The sufficient high levels are sustained for 8 hours. Very high concentrations of cefazolin are also found in the urine."}
{"id": "PMID:1097211", "title": "Shigella strains associated with infantile diarrhea in the Kaohsiung area.", "content": "In 1969 and 1974, 375 Shigella strains were isolated in the Kaohsiung area, and their serotypes and in vitro susceptibility to antimicorbial agents were studied. In 1969, 249 (16.2%) strains were isolated from 1,476 specimens collected from infantile and young children dirrheal cases, whereas 126 strains (11.6%) were isolated from 1,086 specimens in 1969. The isolation rates were higher in May (21.2%), October (20.8%), April (20.6%), August (19.7), November (18.8%) and January (18.5%), than in other months (10.2%-18.0%). Higher incidences of shigellosis appeared in May (22.3%) and April (17.9%) than in the other months during 1974. Shigella sonnei and Shigella flexneri type 2 were most predominant and there was a tendency of increase in number for the former in 1974. From the colicine typing of S. sonnei, it was found that type 8 (40.6%), type 6 (23.4%), and type 12 (17.2%) were the commonly occurring strains associated with the infantile diarrheal disease in the Kaohsiung area. Susceptibility study showed that the resistant rates of S. flexneri to chlroamphenicol (85.5%), streptomycin (81.9%), and tetracycline (61.3%) were much higher than to ampicillin (16.1%), nalidixic acid (14.6%), colistin (4.2%), kanamycin (4.2%), gentamicin (2.8%), cephaloridine (2.4%), and frazolidone (0%). Resistant rates of S. sonnei to 10 antimicrobial agents were as follows: chloramphenicol, 85.9%; tetracycline, 70.2%; streptomycin, 69.9%; nalidixic acid, 16.4%; ampicillin, 13.3%; colistin, 6.3%; kanamycin, 3.9%; gentamicin, 2.5%; cephaloridine, 0%; and frazolidone, 0%.", "contents": "Shigella strains associated with infantile diarrhea in the Kaohsiung area. In 1969 and 1974, 375 Shigella strains were isolated in the Kaohsiung area, and their serotypes and in vitro susceptibility to antimicorbial agents were studied. In 1969, 249 (16.2%) strains were isolated from 1,476 specimens collected from infantile and young children dirrheal cases, whereas 126 strains (11.6%) were isolated from 1,086 specimens in 1969. The isolation rates were higher in May (21.2%), October (20.8%), April (20.6%), August (19.7), November (18.8%) and January (18.5%), than in other months (10.2%-18.0%). Higher incidences of shigellosis appeared in May (22.3%) and April (17.9%) than in the other months during 1974. Shigella sonnei and Shigella flexneri type 2 were most predominant and there was a tendency of increase in number for the former in 1974. From the colicine typing of S. sonnei, it was found that type 8 (40.6%), type 6 (23.4%), and type 12 (17.2%) were the commonly occurring strains associated with the infantile diarrheal disease in the Kaohsiung area. Susceptibility study showed that the resistant rates of S. flexneri to chlroamphenicol (85.5%), streptomycin (81.9%), and tetracycline (61.3%) were much higher than to ampicillin (16.1%), nalidixic acid (14.6%), colistin (4.2%), kanamycin (4.2%), gentamicin (2.8%), cephaloridine (2.4%), and frazolidone (0%). Resistant rates of S. sonnei to 10 antimicrobial agents were as follows: chloramphenicol, 85.9%; tetracycline, 70.2%; streptomycin, 69.9%; nalidixic acid, 16.4%; ampicillin, 13.3%; colistin, 6.3%; kanamycin, 3.9%; gentamicin, 2.5%; cephaloridine, 0%; and frazolidone, 0%."}
{"id": "PMID:1097212", "title": "Non-random chromosome distribution in radial metaphases from the chinese hamster. I. Uncultured cells.", "content": "Chromosome distribution was analyzed in uncultured radial metaphase cells (corneal epithelium, testicular mitotic cells, cells in the diakinesis, and cells in metaphase II) from the Chinese hamster. The hypothesis of random distribution was rejected at the 0.001 level (chi 2/3 = 31.6).--Homologous association was observed for two pairs of chromosomes (3 and 10) in corneal epithelial cells. It was observed for all four cell types. The chromosomes associated in four groups of similarly sized and shaped chromosomes. While group membership did not appear to vary, position within the group was highly variable.--An elevenpoint model of chromosome relationships was constructed from the data.", "contents": "Non-random chromosome distribution in radial metaphases from the chinese hamster. I. Uncultured cells. Chromosome distribution was analyzed in uncultured radial metaphase cells (corneal epithelium, testicular mitotic cells, cells in the diakinesis, and cells in metaphase II) from the Chinese hamster. The hypothesis of random distribution was rejected at the 0.001 level (chi 2/3 = 31.6).--Homologous association was observed for two pairs of chromosomes (3 and 10) in corneal epithelial cells. It was observed for all four cell types. The chromosomes associated in four groups of similarly sized and shaped chromosomes. While group membership did not appear to vary, position within the group was highly variable.--An elevenpoint model of chromosome relationships was constructed from the data."}
{"id": "PMID:1097213", "title": "Chromomere number and its genetic significance in lampbrush chromosomes.", "content": "The chromosomes of three species of salamander belonging to the genus Plethodon have been studied with regard to the absolute length of the haploid set of lampbrush chromosomes and the number and distribution of chromomeres per laploid set of lampbrush chromosomes. Each aspect has been considered in relation to the substantial difference in genome size between P. cinereus (C = 20 pg), P. vehiculum (C = 36.8 pg) and P. dunni (C = 38.8 pg). --Karyotype analyses carried out on unfixed preparations of lampbrush chromosomes showed that the absolute length of a haploid complement of lampbrush chromosomes from medium-sized yolky oocytes is much greater for P. vehiculum and P. dunni than for P. cinereus. Nonetheless, the relative dimensions of chromosomes are nearly identical in all three species. --Chromomers were counted along the whole length of the 14th (shortest) bivalent, and the total number of chromomeres in the haploid set of lampbrush chromosomes was determined by extrapolation on the basis of the known relative length of the 14th bivalent in the respective species. Chromomeres were also counted in measured segments of undamaged lampbrush preprations in which all chromosomes could be clearly identified. The average distance between chromomeres (chromomere distribution) was determined and the total chromomere number was estimated on the basis of measured total length of lampbrush chromosomes in a preparation. Chromomere distribution is the same for lampbrush chromosomes from all 3 species, and since P. vehiculum and P. dunni have longer chromosomes than P. cinereus, it is clear that the former 2 species have many more chromomeres (60-70%) per haploid set of lampbrush chromosomes. The term chromomere was used here in the descriptive sense to define a discrete granule of deoxyribonucleoprotein on the axis of a lampbrush chromosome.--These findings are discussed in relation to what is known of the molecular organization of eukaryotic genomes, and in relation to ideas on gene action and transcription in lampbrush chromosomes.", "contents": "Chromomere number and its genetic significance in lampbrush chromosomes. The chromosomes of three species of salamander belonging to the genus Plethodon have been studied with regard to the absolute length of the haploid set of lampbrush chromosomes and the number and distribution of chromomeres per laploid set of lampbrush chromosomes. Each aspect has been considered in relation to the substantial difference in genome size between P. cinereus (C = 20 pg), P. vehiculum (C = 36.8 pg) and P. dunni (C = 38.8 pg). --Karyotype analyses carried out on unfixed preparations of lampbrush chromosomes showed that the absolute length of a haploid complement of lampbrush chromosomes from medium-sized yolky oocytes is much greater for P. vehiculum and P. dunni than for P. cinereus. Nonetheless, the relative dimensions of chromosomes are nearly identical in all three species. --Chromomers were counted along the whole length of the 14th (shortest) bivalent, and the total number of chromomeres in the haploid set of lampbrush chromosomes was determined by extrapolation on the basis of the known relative length of the 14th bivalent in the respective species. Chromomeres were also counted in measured segments of undamaged lampbrush preprations in which all chromosomes could be clearly identified. The average distance between chromomeres (chromomere distribution) was determined and the total chromomere number was estimated on the basis of measured total length of lampbrush chromosomes in a preparation. Chromomere distribution is the same for lampbrush chromosomes from all 3 species, and since P. vehiculum and P. dunni have longer chromosomes than P. cinereus, it is clear that the former 2 species have many more chromomeres (60-70%) per haploid set of lampbrush chromosomes. The term chromomere was used here in the descriptive sense to define a discrete granule of deoxyribonucleoprotein on the axis of a lampbrush chromosome.--These findings are discussed in relation to what is known of the molecular organization of eukaryotic genomes, and in relation to ideas on gene action and transcription in lampbrush chromosomes."}
{"id": "PMID:1097214", "title": "In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts.", "content": "Methods are described for in situ hybridization of ribosomal DNA from Xenopus laevis, labelled in vitro with 125iodine, to mitotic and lampbrush chromosomes from Triturus cristatus carnifex. The hybridization reaction was carried out in a mixture containing 50% formamide, 4 X SSC, 0.1 M KI, at 37 degrees C, or in 2 X SSC, 0.1 M KI at 65 degrees C. Autoradiographs of mitotic metaphases from 2 males showed labelling over the middle of the short arm of one chromosome IX in each metaphase. In some cases, a region near the end of a longer chromosome was also labelled. In a lampbrush preparations, labelling was confined to a region identified as about 53 units, near the middle of the short arm of both halves of bivalent IX. The usefulness of the technique and the significance of the labelling of only 1 of the 2 chromosomes IX in mitotic preparations are discussed.", "contents": "In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts. Methods are described for in situ hybridization of ribosomal DNA from Xenopus laevis, labelled in vitro with 125iodine, to mitotic and lampbrush chromosomes from Triturus cristatus carnifex. The hybridization reaction was carried out in a mixture containing 50% formamide, 4 X SSC, 0.1 M KI, at 37 degrees C, or in 2 X SSC, 0.1 M KI at 65 degrees C. Autoradiographs of mitotic metaphases from 2 males showed labelling over the middle of the short arm of one chromosome IX in each metaphase. In some cases, a region near the end of a longer chromosome was also labelled. In a lampbrush preparations, labelling was confined to a region identified as about 53 units, near the middle of the short arm of both halves of bivalent IX. The usefulness of the technique and the significance of the labelling of only 1 of the 2 chromosomes IX in mitotic preparations are discussed."}
{"id": "PMID:1097215", "title": "[Identification of the genetic sex chromosomes in the monogenic blowfly Chrysomya rufifacies (Calliphoridae, Diptera)].", "content": "Previous investigations have shown the sex determination in the monogenic blowfly Chrysomya rufifacies to be controlled by a cytologically not discernible homogametry-heterogamety mechanism in the female. Female-producing (thelygenic) females are assumed to be heterozygous for a dominant female sex realizer (F') with sex-predetermining properties, while male-producing (arrhenogenic) females as well as males are supposed to be homozygous for the recessive allele (f). In order to identify the genetic sex chromosomes of C. rufifacies among its five pairs of long euchromatic chromosomes (nos. 1-5) plus one pair of small heterochromatic ones (no. 6), all chromosomes were marked by reciprocal translocations induced by X-ray treatment of adult males. The inheritance of thirteen different heteroxygous translocations has been analyzed. All of the translocations (eleven) between two of the four longer chromosomes did not show sex-linked inheritance, thus demonstrating the autosomal character of the chromosomes nos 1, 2, 3 and 4. The same is true for the translocation T6 (2/6). Therefore the small heterochromatic chromosome no. 6, corresponding to the morphlogically differentiated six chromosomes within the amphogenic calliphorid species, remains without sex determining function in the monogenic fly. This could be confirmed by the analysis of monosomic (monosomy-6) and trisomic (trisomy-6) individuals, which resulted from meiotic non-disfunction in T6/+ translocation heterozygotes. Contrary to these translocations, the heteroxygous 5/2 translocation (T14) exhibited sex-linked inheritance: There was but a very low frequency (0,76 per cent) of recombinants resulting from crossing-over between F'/f and the translocation breakage point in theylgenic F1 T14/+females. The sex-linked inheritance of T14 was confirmed by the progeny of a thelygenic F1 T14/+ female crossed to a homozygous T14/T14 translocation male.Among the offspring of that F1 T14/+ female, which had received the translocation from its father, all of the F2 T14/+ females were thelygenic compared to their arrhenogenic T14/T14 sisters. These results prove that the chromosomes of pair no. 5 genetically act as X'X-XX sex chromosomes in C. rufifacies.", "contents": "[Identification of the genetic sex chromosomes in the monogenic blowfly Chrysomya rufifacies (Calliphoridae, Diptera)]. Previous investigations have shown the sex determination in the monogenic blowfly Chrysomya rufifacies to be controlled by a cytologically not discernible homogametry-heterogamety mechanism in the female. Female-producing (thelygenic) females are assumed to be heterozygous for a dominant female sex realizer (F') with sex-predetermining properties, while male-producing (arrhenogenic) females as well as males are supposed to be homozygous for the recessive allele (f). In order to identify the genetic sex chromosomes of C. rufifacies among its five pairs of long euchromatic chromosomes (nos. 1-5) plus one pair of small heterochromatic ones (no. 6), all chromosomes were marked by reciprocal translocations induced by X-ray treatment of adult males. The inheritance of thirteen different heteroxygous translocations has been analyzed. All of the translocations (eleven) between two of the four longer chromosomes did not show sex-linked inheritance, thus demonstrating the autosomal character of the chromosomes nos 1, 2, 3 and 4. The same is true for the translocation T6 (2/6). Therefore the small heterochromatic chromosome no. 6, corresponding to the morphlogically differentiated six chromosomes within the amphogenic calliphorid species, remains without sex determining function in the monogenic fly. This could be confirmed by the analysis of monosomic (monosomy-6) and trisomic (trisomy-6) individuals, which resulted from meiotic non-disfunction in T6/+ translocation heterozygotes. Contrary to these translocations, the heteroxygous 5/2 translocation (T14) exhibited sex-linked inheritance: There was but a very low frequency (0,76 per cent) of recombinants resulting from crossing-over between F'/f and the translocation breakage point in theylgenic F1 T14/+females. The sex-linked inheritance of T14 was confirmed by the progeny of a thelygenic F1 T14/+ female crossed to a homozygous T14/T14 translocation male.Among the offspring of that F1 T14/+ female, which had received the translocation from its father, all of the F2 T14/+ females were thelygenic compared to their arrhenogenic T14/T14 sisters. These results prove that the chromosomes of pair no. 5 genetically act as X'X-XX sex chromosomes in C. rufifacies."}
{"id": "PMID:1097216", "title": "The bacterial phosphoenolpyruvate: sugar phosphotransferase system.", "content": "The bacterial phosphotransferase system participates in diverse physiological phenomena; its best characterized function is in the group translocation of sugars that are substrates of the system. Such sugars are phosphorylated as they are translocated across the cell membrane. Isolation of different proteins of the phosphotransferase system and reconstitution of the complex shows that in the net transfer of the phosphoryl group from phosphoenolpyruvate to a given sugar the phosphoryl group is sequentially transferred from one protein to another. In all cases so far studied, with one important exception, the phosphoryl group is linked to the proteins through a nitrogen atom in the imidazole ring of a histidyl residue. In the exceptional protein, the phosphoryl group is linked to a carboxy group. An additional function of the phosphotransferase system is to regulate the uptake of sugars that cannot be phosphorylated.", "contents": "The bacterial phosphoenolpyruvate: sugar phosphotransferase system. The bacterial phosphotransferase system participates in diverse physiological phenomena; its best characterized function is in the group translocation of sugars that are substrates of the system. Such sugars are phosphorylated as they are translocated across the cell membrane. Isolation of different proteins of the phosphotransferase system and reconstitution of the complex shows that in the net transfer of the phosphoryl group from phosphoenolpyruvate to a given sugar the phosphoryl group is sequentially transferred from one protein to another. In all cases so far studied, with one important exception, the phosphoryl group is linked to the proteins through a nitrogen atom in the imidazole ring of a histidyl residue. In the exceptional protein, the phosphoryl group is linked to a carboxy group. An additional function of the phosphotransferase system is to regulate the uptake of sugars that cannot be phosphorylated."}
{"id": "PMID:1097217", "title": "The origin of force in skeletal muscle.", "content": "Since the proposal and rapid acceptance of the sliding-filament theory in 1953-1954, numerous suggestions have been made for the cause of the sliding movement. When the amount of overlap is varied by altering the initial length, the maximum tension is directly proportional to, but the speed of shortening under zero load is independent of, the amount of overlap. This suggests strongly that a relative force between thick and thin filaments is produced by independent force-generators distributed within each overlap zone. These force-generators are identified with projections (cross-bridges) on the thick filament, each consisting of part of a myosin molecule. Measurements of the 'tension transients' when the length of a stimulated muscle fibre is suddenly altered show that the range of action of each cross-bridge is 10-15 nm. The travel within a single contraction may be many times greater, so each cross-bridge must act cyclically by attaching, exerting a force and detaching. Details of the tension transients suggest that each cross-bridge makes its movement in two or three steps, each with a potential energy change a few times kT. Each cross-bridge contains also an elastic element in series. It is sufficient, on present evidence, to postulate that the only action of ATP is to dissociate the cross-bridge from the thin filament after it has completed its stroke.", "contents": "The origin of force in skeletal muscle. Since the proposal and rapid acceptance of the sliding-filament theory in 1953-1954, numerous suggestions have been made for the cause of the sliding movement. When the amount of overlap is varied by altering the initial length, the maximum tension is directly proportional to, but the speed of shortening under zero load is independent of, the amount of overlap. This suggests strongly that a relative force between thick and thin filaments is produced by independent force-generators distributed within each overlap zone. These force-generators are identified with projections (cross-bridges) on the thick filament, each consisting of part of a myosin molecule. Measurements of the 'tension transients' when the length of a stimulated muscle fibre is suddenly altered show that the range of action of each cross-bridge is 10-15 nm. The travel within a single contraction may be many times greater, so each cross-bridge must act cyclically by attaching, exerting a force and detaching. Details of the tension transients suggest that each cross-bridge makes its movement in two or three steps, each with a potential energy change a few times kT. Each cross-bridge contains also an elastic element in series. It is sufficient, on present evidence, to postulate that the only action of ATP is to dissociate the cross-bridge from the thin filament after it has completed its stroke."}
{"id": "PMID:1097220", "title": "The effect of methylphenidate on sensory perception in varying degrees of hyperkinetic behaviour.", "content": "In a double-blind study of methylphenidate, hyperkinetic children of varying levels of age and degree of hyperactivity were assessed on subtests of the Illinois Test of Psycholinguistic Ability (ITPA). The ITPA was supplemented with a combination of monosensory, bisensory, and trisensory stimuli presentations. Methylphenidate improved the ITPA's visual, auditory and tactile age scores of children who exhibited a more marked degree of hyperkinetic behaviour. That improvement was also observed in the recognition of sensory stimuli presented in a monosensory fashion. Similarly, the children with a more marked degree of hyperkinesis improved their stimuli recognition rate significantly on methylphenidate when stimuli were presented to bisensory and trisensory perceptual modalities simultaneously. It was concluded that drug improvement on sensory perception seemed to be more related to the degree of hyperkinetic behavior manifested than to chronological age. Further research of degree of hyperkinesis, age-level, sensory modality and drug administration was suggested.", "contents": "The effect of methylphenidate on sensory perception in varying degrees of hyperkinetic behaviour. In a double-blind study of methylphenidate, hyperkinetic children of varying levels of age and degree of hyperactivity were assessed on subtests of the Illinois Test of Psycholinguistic Ability (ITPA). The ITPA was supplemented with a combination of monosensory, bisensory, and trisensory stimuli presentations. Methylphenidate improved the ITPA's visual, auditory and tactile age scores of children who exhibited a more marked degree of hyperkinetic behaviour. That improvement was also observed in the recognition of sensory stimuli presented in a monosensory fashion. Similarly, the children with a more marked degree of hyperkinesis improved their stimuli recognition rate significantly on methylphenidate when stimuli were presented to bisensory and trisensory perceptual modalities simultaneously. It was concluded that drug improvement on sensory perception seemed to be more related to the degree of hyperkinetic behavior manifested than to chronological age. Further research of degree of hyperkinesis, age-level, sensory modality and drug administration was suggested."}
{"id": "PMID:1097221", "title": "Loxapine succinate: a controlled double-blind study in chronic schizophrenia.", "content": "Loxapine succinate was compared to chlorpromazine in a 12-week double-blind study of 50 hospitalized chronic schizophrenic patients. Statistical analyses of BPRS scores showed significant improvemement for several items and factors in both groups of patients. Both drugs significantly decreased severity of illness on the CGI scale. On the NOSIE scale, \"manifest psychosis\" was improved significantly with chlorpromazine and \"global severity\" with loxapine succinate. There were no significant treatment differences in BPRS, CGI, or Nosie items or factors or in the reduction of overall psychopathology. The side effects associated with the study drugs differed little with respect to incidence, number, severity, and type. The most frequently reported symptoms in both groups were behavioral, extrapyramidal, and sedative. Analyses of vital signs and clinical laboratory data revealed no evidence of serious untoward effects.", "contents": "Loxapine succinate: a controlled double-blind study in chronic schizophrenia. Loxapine succinate was compared to chlorpromazine in a 12-week double-blind study of 50 hospitalized chronic schizophrenic patients. Statistical analyses of BPRS scores showed significant improvemement for several items and factors in both groups of patients. Both drugs significantly decreased severity of illness on the CGI scale. On the NOSIE scale, \"manifest psychosis\" was improved significantly with chlorpromazine and \"global severity\" with loxapine succinate. There were no significant treatment differences in BPRS, CGI, or Nosie items or factors or in the reduction of overall psychopathology. The side effects associated with the study drugs differed little with respect to incidence, number, severity, and type. The most frequently reported symptoms in both groups were behavioral, extrapyramidal, and sedative. Analyses of vital signs and clinical laboratory data revealed no evidence of serious untoward effects."}
{"id": "PMID:1097222", "title": "The treatment of cluster headaches with prednisone.", "content": "Nineteen patients obstinate with cluster headaches whose pain was not mitigated by standard treatment (Methysergide, caffeine, ergotamine preparation, phenobarbital and analgesics) underwent a double blind control study with single crossover for the evaluation of prednisone therapy. Compared to placebo, a single oral dose of prednisone in 17 cases produced sustained improvement. Maintenance administration of prednisone was also effective in decreasing the frequency of attacks; however a single dose of the steroid when headaches began was effective.", "contents": "The treatment of cluster headaches with prednisone. Nineteen patients obstinate with cluster headaches whose pain was not mitigated by standard treatment (Methysergide, caffeine, ergotamine preparation, phenobarbital and analgesics) underwent a double blind control study with single crossover for the evaluation of prednisone therapy. Compared to placebo, a single oral dose of prednisone in 17 cases produced sustained improvement. Maintenance administration of prednisone was also effective in decreasing the frequency of attacks; however a single dose of the steroid when headaches began was effective."}
{"id": "PMID:1097218", "title": "A foreign body (chicken bone) in the rectum causing extensive perirectal and scrotal abscess: report of a case.", "content": "A case of foreign-body perforation of the rectum with extensive necrotizing fasciitis is presented and the literature briefly reviewed. The peculiarity of the case was brought out because of the nature of the foreign body(chicken bone) and the extensiveness of the process. The increases risk of swallowing a foreign body if dentures are worn is pointed out.", "contents": "A foreign body (chicken bone) in the rectum causing extensive perirectal and scrotal abscess: report of a case. A case of foreign-body perforation of the rectum with extensive necrotizing fasciitis is presented and the literature briefly reviewed. The peculiarity of the case was brought out because of the nature of the foreign body(chicken bone) and the extensiveness of the process. The increases risk of swallowing a foreign body if dentures are worn is pointed out."}
{"id": "PMID:1097228", "title": "[Infection with \"enteritis salmonella\" at non-intestinal sites (author's transl)].", "content": "So-called \"enteritis Salmonella\" was isolated in five cases from extra-intestinal specimens (about 3% of all primary Salmonella isolations in one year). The organisms were: S. typhimurium, S. enteritidis, S. java, S. panama, and S. infantis. In four of the five cases the clinical picture was not that of a Salmonella infection. Focal manifestations were a knee-joint empyema after long-term treatment with glucocorticoids, pleuritis in Hodgkin's disease, osteomyelitis of the head of the tibia, and meningitis in a child. In a 72-year-old man, acute renal failure occurred after enteritis, following which the Salmonella was isolated from the respiratory tract for many weeks.", "contents": "[Infection with \"enteritis salmonella\" at non-intestinal sites (author's transl)]. So-called \"enteritis Salmonella\" was isolated in five cases from extra-intestinal specimens (about 3% of all primary Salmonella isolations in one year). The organisms were: S. typhimurium, S. enteritidis, S. java, S. panama, and S. infantis. In four of the five cases the clinical picture was not that of a Salmonella infection. Focal manifestations were a knee-joint empyema after long-term treatment with glucocorticoids, pleuritis in Hodgkin's disease, osteomyelitis of the head of the tibia, and meningitis in a child. In a 72-year-old man, acute renal failure occurred after enteritis, following which the Salmonella was isolated from the respiratory tract for many weeks."}
{"id": "PMID:1097230", "title": "[Pseudo-LE syndrome after treatment with Venopyronum dragees] (author's transl)", "content": "During the past three years a pseudo-LE syndrome (with antimitochondrial antibodies) was diagnosed in 14 patients. Information from the patients and their doctors disclosed that twelve of them had taken Venopyronum dragees over a period of two months to five years before onset of first symptoms. Two of the patients died of nonspecific myocarditis. Clinical investigations strongly suggest that the drug may have played a role in the pathogenesis of the pseudo-LE syndrome which is likely to take a severe course if, as in the two patients, the cardiovascular system is involved.", "contents": "[Pseudo-LE syndrome after treatment with Venopyronum dragees] (author's transl). During the past three years a pseudo-LE syndrome (with antimitochondrial antibodies) was diagnosed in 14 patients. Information from the patients and their doctors disclosed that twelve of them had taken Venopyronum dragees over a period of two months to five years before onset of first symptoms. Two of the patients died of nonspecific myocarditis. Clinical investigations strongly suggest that the drug may have played a role in the pathogenesis of the pseudo-LE syndrome which is likely to take a severe course if, as in the two patients, the cardiovascular system is involved."}
{"id": "PMID:1097233", "title": "Naproxen: a review of its pharmacological properties and therapeutic efficacy and use.", "content": "Naproxen2, (+)-6-methoxy-alpha-methyl-2-naphthalene acetic acid, is a new non-steroidal anti=inflammatory agent advocated for use in rheumatiod arthritis, degenerative joint disease and ankylosing spondylitis. Published data suggest that in rheumatiod arthritis, naproxen 500mg daily comparable in efficacy with moderate doses of aspirin (3.6 to 4g daily) or 150mg daily of indomethacin, but generally causes fewer and milder side-effects than these drugs at the dosages used and can be given less frequently (12-hourly). Encouraging intial results have been reproted from its use in other inflammatory joint disorders, including acute gout and juvenile rheumatiod arthritis. It has compared favourably with indomethacin in ostioarthrosis of the hip of knee. Its exact place in the management of ankylosing spondylitis remains to be determined.", "contents": "Naproxen: a review of its pharmacological properties and therapeutic efficacy and use. Naproxen2, (+)-6-methoxy-alpha-methyl-2-naphthalene acetic acid, is a new non-steroidal anti=inflammatory agent advocated for use in rheumatiod arthritis, degenerative joint disease and ankylosing spondylitis. Published data suggest that in rheumatiod arthritis, naproxen 500mg daily comparable in efficacy with moderate doses of aspirin (3.6 to 4g daily) or 150mg daily of indomethacin, but generally causes fewer and milder side-effects than these drugs at the dosages used and can be given less frequently (12-hourly). Encouraging intial results have been reproted from its use in other inflammatory joint disorders, including acute gout and juvenile rheumatiod arthritis. It has compared favourably with indomethacin in ostioarthrosis of the hip of knee. Its exact place in the management of ankylosing spondylitis remains to be determined."}
{"id": "PMID:1097234", "title": "Clotrimazole: a review of its antifungal activity and therapeutic efficacy.", "content": "Clotrimazole 2, a synthetic imidazole derivative, is primarily used locally in the treatment of vaginal and skin infections due to yeasts and dermatophytes. In vitro, it is most active against Candida spp., Trichophyton spp., Microsporum spp. and Malazzesia fuffur (Pityrosporon orbiculare). In addition, it has some in vitro activity against certain Gram-positive bacteria, and at very high concentrations has activity against Trichomonas spp. In the treatment of vaginal candidiasis, clotrimazole vaginal tablets have produced cure rates comparable with those of conventional nystatin vaginal tablets. There have been no published comparisons with nystatin vaginal cream or foaming vaginal tablets - nystatin dosage forms preferred by some clinicians. Cootrimazole has also been successful in patients who had failed to respond to other antifungal agents such as nystatin and amphotericin B. Results in trichomonal vaginitis are not impressive. Skin infections caused by Candida or dermatophytes have been effectively treated with topical application of clotrimazole. In comparative trials, clotrimazole cream has been as effective as Whitfield's ointment and tolnaftate in the treatment of dermatophytoses, and as effective as nystatin in cutaneous candidiasis. Clotrimazole topical preparations are generally well tolerated, but local irritation has necessitated withdrawal of therapy in a few cases. Candidal septicemia and urinary and pulmonary candidiasis have been cured with oral clotrimazole therapy. Results in other types of serious fungal infections, including pulmonary aspergillosis, have been disappointing. A limiting factor in oral clotrimazole therapy is the high incidence of gastro-intestinal disturbances and neurological reactions.", "contents": "Clotrimazole: a review of its antifungal activity and therapeutic efficacy. Clotrimazole 2, a synthetic imidazole derivative, is primarily used locally in the treatment of vaginal and skin infections due to yeasts and dermatophytes. In vitro, it is most active against Candida spp., Trichophyton spp., Microsporum spp. and Malazzesia fuffur (Pityrosporon orbiculare). In addition, it has some in vitro activity against certain Gram-positive bacteria, and at very high concentrations has activity against Trichomonas spp. In the treatment of vaginal candidiasis, clotrimazole vaginal tablets have produced cure rates comparable with those of conventional nystatin vaginal tablets. There have been no published comparisons with nystatin vaginal cream or foaming vaginal tablets - nystatin dosage forms preferred by some clinicians. Cootrimazole has also been successful in patients who had failed to respond to other antifungal agents such as nystatin and amphotericin B. Results in trichomonal vaginitis are not impressive. Skin infections caused by Candida or dermatophytes have been effectively treated with topical application of clotrimazole. In comparative trials, clotrimazole cream has been as effective as Whitfield's ointment and tolnaftate in the treatment of dermatophytoses, and as effective as nystatin in cutaneous candidiasis. Clotrimazole topical preparations are generally well tolerated, but local irritation has necessitated withdrawal of therapy in a few cases. Candidal septicemia and urinary and pulmonary candidiasis have been cured with oral clotrimazole therapy. Results in other types of serious fungal infections, including pulmonary aspergillosis, have been disappointing. A limiting factor in oral clotrimazole therapy is the high incidence of gastro-intestinal disturbances and neurological reactions."}
{"id": "PMID:1097236", "title": "Ihibition of the pre-ovulatory proestrous gonadotropin surge, ovulation and pregnancy with a peptide analogue of luteinizing hormone releasing hormone.", "content": "D-[PHE]-2-D[ALA]-6-LRH (Wy-18,185), an analogue of synthetic hypothalamic LRH, was evaluated for its anti-fertility properties. The compound dampens the preovulatory proestrous surge of serum LH and FSH in rats, is an active anti-ovulatory agent in both rats and rabbits and prevents pregnancy when administered pre-coitally to rats. Additionally, the compound suppresses the ovulatory event in rats only during the cycle in which it is acutely administered, does not interfere with the rat vaginal cycle and is devoid of any effects on body, ovarian and uterine weights when given acutely. The compound is a moderate LRH agonist in rats but does not induce ovulation in rats or rabbits.", "contents": "Ihibition of the pre-ovulatory proestrous gonadotropin surge, ovulation and pregnancy with a peptide analogue of luteinizing hormone releasing hormone. D-[PHE]-2-D[ALA]-6-LRH (Wy-18,185), an analogue of synthetic hypothalamic LRH, was evaluated for its anti-fertility properties. The compound dampens the preovulatory proestrous surge of serum LH and FSH in rats, is an active anti-ovulatory agent in both rats and rabbits and prevents pregnancy when administered pre-coitally to rats. Additionally, the compound suppresses the ovulatory event in rats only during the cycle in which it is acutely administered, does not interfere with the rat vaginal cycle and is devoid of any effects on body, ovarian and uterine weights when given acutely. The compound is a moderate LRH agonist in rats but does not induce ovulation in rats or rabbits."}
{"id": "PMID:1097237", "title": "Studies on the mechanism controlling growth hormone release induced by chlorpromazine in the anesthetized rat.", "content": "In intact urethane-anesthetized rats, plasma growth hormone (GH) levels were low but increased significantly following intravenous injection of chlorpromazine.  Plasma GH levels were significantly elevated in rats with hypothalamic cuts such as complete  deafferentiation, anterior cut and antero-lateral cut, whereas plasma GH levels in rats with posterior cut or postero-lateral cut were not significantly different from those in rats with sham-operation.  Intravenous injection of chlorpromazine caused an increase of plasma GH in rats with any type of hypothalamic cut.  However, the maximum increments of plasma GH following chlorpromazine were larger in rats with antero-lateral cut and smaller in rats with posterior cut than in rats with sham-operation.  These results suggest that extrahypothalamic inhibiting and stimulating neurons influence the regulatory mechanism of rat GH secretion through anterior and posterior routes to the hypothalamus respectively.", "contents": "Studies on the mechanism controlling growth hormone release induced by chlorpromazine in the anesthetized rat. In intact urethane-anesthetized rats, plasma growth hormone (GH) levels were low but increased significantly following intravenous injection of chlorpromazine.  Plasma GH levels were significantly elevated in rats with hypothalamic cuts such as complete  deafferentiation, anterior cut and antero-lateral cut, whereas plasma GH levels in rats with posterior cut or postero-lateral cut were not significantly different from those in rats with sham-operation.  Intravenous injection of chlorpromazine caused an increase of plasma GH in rats with any type of hypothalamic cut.  However, the maximum increments of plasma GH following chlorpromazine were larger in rats with antero-lateral cut and smaller in rats with posterior cut than in rats with sham-operation.  These results suggest that extrahypothalamic inhibiting and stimulating neurons influence the regulatory mechanism of rat GH secretion through anterior and posterior routes to the hypothalamus respectively."}
{"id": "PMID:1097238", "title": "Effect of prostaglandin F2alpha upon ovulation and LH, FSH and prolactin secretion in chlorpromazine blocked rats.", "content": "The ovulation-inducing and LH, FSH and protein releasing activities of prostaglandin F2alpha (PG F2alpha) were investigated, using the female rats which had ovulation blocked by chlorpromazine (CPZ). Serum LH, FSH and prolactin concentrations were measured with NIAMD-rat-radioimmunoassay kits. The number of ova present in the fallopian tubes was investigated microscopically. Subcutaneous injection of 0.4-1.6 mg of PG F2alpha at 3:00 p.m. to CPZ-blocked proestrous rats induced ovulation in 80% of the animals during presumptive estrus. However, the percentage of ovulating rats was reduced in 40-60% of the animals when the same doses of PG F2alpha were injected intravenously. All control animals receiving the vehicle alone did not ovulate. The number of ova was not related to the dose of PG F2alpha, but the percentage of ovulating rats was. The serum LH peak was observed 10 min after the intravenous injection and 60-180 min after the subcutaneous injection of PG F2alpha. The peak in serum FSH and prolactin occurred simultaneously 180 min after both subcutaneous and intravenous injection of PG F2alpha. It is concluded from these data that PG F2alpha at dose levels used, may directly affect the hypothalamo-pituitary axis and consequently induce ovulation. These results further support the conception that the prostaglandins (PGs) have a central effect stimulating LH, FSH and prolactin release from the anterior pituitary.", "contents": "Effect of prostaglandin F2alpha upon ovulation and LH, FSH and prolactin secretion in chlorpromazine blocked rats. The ovulation-inducing and LH, FSH and protein releasing activities of prostaglandin F2alpha (PG F2alpha) were investigated, using the female rats which had ovulation blocked by chlorpromazine (CPZ). Serum LH, FSH and prolactin concentrations were measured with NIAMD-rat-radioimmunoassay kits. The number of ova present in the fallopian tubes was investigated microscopically. Subcutaneous injection of 0.4-1.6 mg of PG F2alpha at 3:00 p.m. to CPZ-blocked proestrous rats induced ovulation in 80% of the animals during presumptive estrus. However, the percentage of ovulating rats was reduced in 40-60% of the animals when the same doses of PG F2alpha were injected intravenously. All control animals receiving the vehicle alone did not ovulate. The number of ova was not related to the dose of PG F2alpha, but the percentage of ovulating rats was. The serum LH peak was observed 10 min after the intravenous injection and 60-180 min after the subcutaneous injection of PG F2alpha. The peak in serum FSH and prolactin occurred simultaneously 180 min after both subcutaneous and intravenous injection of PG F2alpha. It is concluded from these data that PG F2alpha at dose levels used, may directly affect the hypothalamo-pituitary axis and consequently induce ovulation. These results further support the conception that the prostaglandins (PGs) have a central effect stimulating LH, FSH and prolactin release from the anterior pituitary."}
{"id": "PMID:1097239", "title": "Specific and nonspecific multiple unit activities during the onset of pentylentetrazol seizures. I. Intact animals.", "content": "A quantitative evaluation of specific and nonspecific multiple unit activity (MUA) before and during pentylenetetrazol-induced EEG tonic-clonic discharges (OTCD) was made at four levels of the CNS: cortical, thalamic, mesencephalic, and pontine. In addition, MUA of sciatic nerve was recorded to detect possible spinal cord efferent discharges. At all levels, sustained increase in specific and nonspecific multiple unit activity preceded EEG and sciatic nerve discharges induced by  pentylenetetrazol. Increments in nonspecific MUA occurred sooner and were larger than those of specific MUA, and increments in mesencephalic nonspecific MUA occurred sooner and were larger than those of other cortical, thalamic, and pontine nonspecific MUAs. This sequence of neuronal activation suggests that pentylenetetrazol seizures are initiated in nonspecific structures at the mesencephalic level.", "contents": "Specific and nonspecific multiple unit activities during the onset of pentylentetrazol seizures. I. Intact animals. A quantitative evaluation of specific and nonspecific multiple unit activity (MUA) before and during pentylenetetrazol-induced EEG tonic-clonic discharges (OTCD) was made at four levels of the CNS: cortical, thalamic, mesencephalic, and pontine. In addition, MUA of sciatic nerve was recorded to detect possible spinal cord efferent discharges. At all levels, sustained increase in specific and nonspecific multiple unit activity preceded EEG and sciatic nerve discharges induced by  pentylenetetrazol. Increments in nonspecific MUA occurred sooner and were larger than those of specific MUA, and increments in mesencephalic nonspecific MUA occurred sooner and were larger than those of other cortical, thalamic, and pontine nonspecific MUAs. This sequence of neuronal activation suggests that pentylenetetrazol seizures are initiated in nonspecific structures at the mesencephalic level."}
{"id": "PMID:1097240", "title": "Effects of hippocampal afterdischarges on Purkinje cell activity.", "content": "Hippocampal afterdischarges can induce increased Purkinje cell activity that characteristically shows a pronounced acceleration during the period of the afterdischarges. Increased Purkinje cell activity may continue for many seconds after termination of the hippocampal activity. Typical frequency histograms are presented. A short period of hippocampal afterdischarges (under 25 sec) may occur with few if any alterations in cerebral activity and vice versa. However, such afterdischarges can produce changes in cerebellar activity as shown by enhanced Purkinje cell activity. These data support the view that the nucleus tegmenti pontis may be prominently involved in relaying hippocampal afterdischarges to the cerebellum whereas much of the neocortical seizure activity is relayed trough the pons, inferior olive, and lateral reticular nucleus, rather than through nucleus tegmenti pontis. EEG tracings taken from these areas illustrate the findings.", "contents": "Effects of hippocampal afterdischarges on Purkinje cell activity. Hippocampal afterdischarges can induce increased Purkinje cell activity that characteristically shows a pronounced acceleration during the period of the afterdischarges. Increased Purkinje cell activity may continue for many seconds after termination of the hippocampal activity. Typical frequency histograms are presented. A short period of hippocampal afterdischarges (under 25 sec) may occur with few if any alterations in cerebral activity and vice versa. However, such afterdischarges can produce changes in cerebellar activity as shown by enhanced Purkinje cell activity. These data support the view that the nucleus tegmenti pontis may be prominently involved in relaying hippocampal afterdischarges to the cerebellum whereas much of the neocortical seizure activity is relayed trough the pons, inferior olive, and lateral reticular nucleus, rather than through nucleus tegmenti pontis. EEG tracings taken from these areas illustrate the findings."}
{"id": "PMID:1097241", "title": "Increased cholesterol-ester formation during forced cholesterol synthesis in rat hepatocytes.", "content": "By comparing the incorporation of 3H20 and [14C]mevalonate into cholesterol in suspensions of rat hepatocytes, it was calculated that the cholesterol biosynthesis could be stimulated 4--7-fold by addition of mevalonate. The addition of 3.3--6.7 mM mevalonate also caused a 5--6-fold increase in the proportion of newly synthesized cholesterol that was esterifield. The esterification of radioactive cholesterol, entering the cells by exchange with surrounding plasma lipoproteins was also increased, indicating that a true increase in the rate of cholesterol ester formation rather than a more selective utilization of newly synthesized cholesterol for esterification, occurred. The increase in cholesterol esterification was not abolished by cycloheximide, indicating that it did not require an increased synthesis of cholesterol esterifying enzyme. Instead the data suggest that the supply of cholesterol to the esterifiable pool may be an important factor, regulating the rate of cholesterol ester formation in rat liver. The addition of 0.5 mM oleic acid to the medium did not increase the degree of cholesterol esterification significantly, whereas 2 mM oleic acid bound to 1% albumin increased the proportion of newly synthesized cholesterol that was esterified, by about 70%. The cells secreted radioactive cholesterol esters into the medium. Cycloheximide inhibited this secretion to about 80% but did not affect the rate at which newly synthesized cholesterol was transferred to surrounding plasma lipoproteins.", "contents": "Increased cholesterol-ester formation during forced cholesterol synthesis in rat hepatocytes. By comparing the incorporation of 3H20 and [14C]mevalonate into cholesterol in suspensions of rat hepatocytes, it was calculated that the cholesterol biosynthesis could be stimulated 4--7-fold by addition of mevalonate. The addition of 3.3--6.7 mM mevalonate also caused a 5--6-fold increase in the proportion of newly synthesized cholesterol that was esterifield. The esterification of radioactive cholesterol, entering the cells by exchange with surrounding plasma lipoproteins was also increased, indicating that a true increase in the rate of cholesterol ester formation rather than a more selective utilization of newly synthesized cholesterol for esterification, occurred. The increase in cholesterol esterification was not abolished by cycloheximide, indicating that it did not require an increased synthesis of cholesterol esterifying enzyme. Instead the data suggest that the supply of cholesterol to the esterifiable pool may be an important factor, regulating the rate of cholesterol ester formation in rat liver. The addition of 0.5 mM oleic acid to the medium did not increase the degree of cholesterol esterification significantly, whereas 2 mM oleic acid bound to 1% albumin increased the proportion of newly synthesized cholesterol that was esterified, by about 70%. The cells secreted radioactive cholesterol esters into the medium. Cycloheximide inhibited this secretion to about 80% but did not affect the rate at which newly synthesized cholesterol was transferred to surrounding plasma lipoproteins."}
{"id": "PMID:1097242", "title": "Stimultaneous purification of Escherichia coli termination factor rho, RNAase III and RNAase H.", "content": "This communication describes a method for the stimultaneous purification of Escherichia coli termination factor rho, RNAase III and RNAase H, which is rapid, reproducible and high in yield. Depending on how cells are grown 0.5 to 1 mg of RNAase III, 1 to 2 mg of RNAase H and 1 to 2 mg of rho are obtained from 100 g wet cells. RNAase III and rho are pure proteins, and RNAase H 80% pure. In addition it is shown that pure RNAase III degrades only RNA. RNA duplexes, and is responsible for the sizing of early T7 mRNA. The active form of RNAase III is composed of two identical subunits having a molecular weight of 23 500. Native RNAase H which specifically hydrolyses the RNA moiety of an RNA. DNA hybrid, is a single polypeptide chain with a molecular weight of 21 000 The amino acid composition of termination factor rho is also reported.", "contents": "Stimultaneous purification of Escherichia coli termination factor rho, RNAase III and RNAase H. This communication describes a method for the stimultaneous purification of Escherichia coli termination factor rho, RNAase III and RNAase H, which is rapid, reproducible and high in yield. Depending on how cells are grown 0.5 to 1 mg of RNAase III, 1 to 2 mg of RNAase H and 1 to 2 mg of rho are obtained from 100 g wet cells. RNAase III and rho are pure proteins, and RNAase H 80% pure. In addition it is shown that pure RNAase III degrades only RNA. RNA duplexes, and is responsible for the sizing of early T7 mRNA. The active form of RNAase III is composed of two identical subunits having a molecular weight of 23 500. Native RNAase H which specifically hydrolyses the RNA moiety of an RNA. DNA hybrid, is a single polypeptide chain with a molecular weight of 21 000 The amino acid composition of termination factor rho is also reported."}
{"id": "PMID:1097243", "title": "Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants.", "content": "Genetic analysis of venturicidin-resistant mutants has revealed the presence of both nuclear and mitochondrial genes responsible for determining venturicidin sensitivity/resistance in Saccharomyces cerevisiae. Recombination studies show that the mutation with phenotype VENR is situated at mitochondrial locus OL I and is therefore extremely useful of future genetic manipulations as it gives a unique phenotype to this locus distinguishable from the second oligomycin locus OL II. The mutations with phenotype VENR OLYR are linked to oligomycin locus OL I and have been allocated a new mitochondrial locus, namely OL III. Three factor croses involving the venturicidin mutations at loci OL I and OL III have shown them to freely recombine with the other mitochondrial loci R I, R III and OL II. The mitochondrial genetic map is therefore represented as four 'recombinational linkage groups'. A fifth linkage group is also specified for mutants with phenotype VENR TETR, and is probably located on a separate DNA molecule from the four other groups.", "contents": "Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants. Genetic analysis of venturicidin-resistant mutants has revealed the presence of both nuclear and mitochondrial genes responsible for determining venturicidin sensitivity/resistance in Saccharomyces cerevisiae. Recombination studies show that the mutation with phenotype VENR is situated at mitochondrial locus OL I and is therefore extremely useful of future genetic manipulations as it gives a unique phenotype to this locus distinguishable from the second oligomycin locus OL II. The mutations with phenotype VENR OLYR are linked to oligomycin locus OL I and have been allocated a new mitochondrial locus, namely OL III. Three factor croses involving the venturicidin mutations at loci OL I and OL III have shown them to freely recombine with the other mitochondrial loci R I, R III and OL II. The mitochondrial genetic map is therefore represented as four 'recombinational linkage groups'. A fifth linkage group is also specified for mutants with phenotype VENR TETR, and is probably located on a separate DNA molecule from the four other groups."}
{"id": "PMID:1097244", "title": "Glyceraldehyde phosphate at the reducing terminus of Salmonella Q haptens. Salmonella montevideo.", "content": "The O antigen polysaccharide of Salmonella montevideo was isolated from a core-defective mutant by the phenol/water procedure, and was suspected to contain phosphomonester and cyclic phosphodiester at its reducing end in anology to the O hapten from Salmonella typhimurium (Kent and Obsborn, 1968. Therefore, it was chromatographed on a DEAE-cellulose column. Whereas one part eluted with water the other part of the polysaccharide could only be eluted with buffer. Both fractions were further purified on Sephadex G100 and contained mannose, glucose, N-acetylglucosamine and phosphate in a molar ratio of 4:1:1: less than 0.1. In order to specifically label the reducing end phosphate was removed enzymatically, or the presumed cyclic diester was cleaved by mild hydrolysis, and the fractions were reduced with sodium horo[3H]hydride. Both fractions yield mainly [3H]glycerol after hydrolysis and paper chromatogaphy. In addition, [3H]mannitol and [H]monohydroxyacetone could be identified by paper chromatography and were concluded to be the result of phosphate migration and beta-elimination reactions taking place during the isolation procedure and the various treatments prior to sodium boro[3H]hydride reduction. These findings in addition to periodate oxidation studies indicated that the O antigen polysaccharide of Salmonella montevideo had glyceraldehyde phosphate at its reducing end. From the incorporation of 3H into the polysaccharide the O antigen was calculated to consist of about 19 repeating units of 6 sugar residues each.", "contents": "Glyceraldehyde phosphate at the reducing terminus of Salmonella Q haptens. Salmonella montevideo. The O antigen polysaccharide of Salmonella montevideo was isolated from a core-defective mutant by the phenol/water procedure, and was suspected to contain phosphomonester and cyclic phosphodiester at its reducing end in anology to the O hapten from Salmonella typhimurium (Kent and Obsborn, 1968. Therefore, it was chromatographed on a DEAE-cellulose column. Whereas one part eluted with water the other part of the polysaccharide could only be eluted with buffer. Both fractions were further purified on Sephadex G100 and contained mannose, glucose, N-acetylglucosamine and phosphate in a molar ratio of 4:1:1: less than 0.1. In order to specifically label the reducing end phosphate was removed enzymatically, or the presumed cyclic diester was cleaved by mild hydrolysis, and the fractions were reduced with sodium horo[3H]hydride. Both fractions yield mainly [3H]glycerol after hydrolysis and paper chromatogaphy. In addition, [3H]mannitol and [H]monohydroxyacetone could be identified by paper chromatography and were concluded to be the result of phosphate migration and beta-elimination reactions taking place during the isolation procedure and the various treatments prior to sodium boro[3H]hydride reduction. These findings in addition to periodate oxidation studies indicated that the O antigen polysaccharide of Salmonella montevideo had glyceraldehyde phosphate at its reducing end. From the incorporation of 3H into the polysaccharide the O antigen was calculated to consist of about 19 repeating units of 6 sugar residues each."}
{"id": "PMID:1097245", "title": "Ribosomal RNA genes in the amoebal and plasmodial forms of the slime mould Physarum polycephalum.", "content": "1. The degree of homology between ribosomal RNA isolated from microplasmodia and amoebae of the slime mould Physarum polycephalum has been determined by competive hybridisation of the RNA from the two sources to homologous DNA in solution. The extent of competition was measured both by hybridisation to saturation and by following the kinetics of hybrid formation. In each case competition was found to be 100%, indicating that the ribosomal RNAs from the two, quite different vegetative forms of the organism exhibit a high degree of homology and are probably transcribed from the same genes. 2. The relationship between the amount of nuclear DNA that codes for ribosomal RNA (rDNA) and ploidy has been investigated in three strains of P. polycephalum which exhibit a 1:2:5 variation in the amount of DNA per nucleus. Ribosomal RNA saturation values were determined by hybridisation to DNA isolated from prophase nuclei of plasmodia. The proportion of rDNA was found to be constant at 0.16--0.18% of the total genome in the three strains.", "contents": "Ribosomal RNA genes in the amoebal and plasmodial forms of the slime mould Physarum polycephalum. 1. The degree of homology between ribosomal RNA isolated from microplasmodia and amoebae of the slime mould Physarum polycephalum has been determined by competive hybridisation of the RNA from the two sources to homologous DNA in solution. The extent of competition was measured both by hybridisation to saturation and by following the kinetics of hybrid formation. In each case competition was found to be 100%, indicating that the ribosomal RNAs from the two, quite different vegetative forms of the organism exhibit a high degree of homology and are probably transcribed from the same genes. 2. The relationship between the amount of nuclear DNA that codes for ribosomal RNA (rDNA) and ploidy has been investigated in three strains of P. polycephalum which exhibit a 1:2:5 variation in the amount of DNA per nucleus. Ribosomal RNA saturation values were determined by hybridisation to DNA isolated from prophase nuclei of plasmodia. The proportion of rDNA was found to be constant at 0.16--0.18% of the total genome in the three strains."}
{"id": "PMID:1097246", "title": "The effects of overloading in density-gradient centrifugation.", "content": "The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable from the Svedberg equation. The two typical effects of overloading are dislocation of the zone mass centres and changes in the zone shapes. It is found that the magnitude of the calculated sedimentation coefficients increases nearly linearly with increasing sample load. The changes in zone shapes are found to depend on the specific load and two different patterns may be distinguished. The zone of the sample component which causes the overloading is defined as primarily overloaded and the others as secondarily overloaded. In primarily overloaded zones the original Gaussian shape is lost, while in secondarily overloaded zones the Gaussian zone shape is maintained, although a zone broadening is seen. Extreme high loads are found to be able to divide single zones. As a whole these experiments show that evidence for a non-overloaded set of experimental conditions must be provided, when density gradient centrifugation is used for determination of sedimentation coefficients. For preparative gradient centrifugations the power of resolution will decrease with increasing sample load. A simple method to detect overloading in density gradient centrifugations is described.", "contents": "The effects of overloading in density-gradient centrifugation. The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable from the Svedberg equation. The two typical effects of overloading are dislocation of the zone mass centres and changes in the zone shapes. It is found that the magnitude of the calculated sedimentation coefficients increases nearly linearly with increasing sample load. The changes in zone shapes are found to depend on the specific load and two different patterns may be distinguished. The zone of the sample component which causes the overloading is defined as primarily overloaded and the others as secondarily overloaded. In primarily overloaded zones the original Gaussian shape is lost, while in secondarily overloaded zones the Gaussian zone shape is maintained, although a zone broadening is seen. Extreme high loads are found to be able to divide single zones. As a whole these experiments show that evidence for a non-overloaded set of experimental conditions must be provided, when density gradient centrifugation is used for determination of sedimentation coefficients. For preparative gradient centrifugations the power of resolution will decrease with increasing sample load. A simple method to detect overloading in density gradient centrifugations is described."}
{"id": "PMID:1097247", "title": "The \"hidden ligand\" of the galactose-binding protein.", "content": "Following tryptophan fluorescence of the galactose-binding during dissociation of the ligand it has been found that glucose dissociates with a half life of less than 5 s. Similarly, fast dissociation was also observed by following release of radioactively labelled glucose from Sepharose-coupled galactose-binding protein upon dilution. Accordingly, a previous claim that the galactose-binding protein contains glucose as a non-dissociable \"hidden ligand\" [G. Richarme and A. Kepes (1974) Eur. J. Biochem. 45, 127-133] has to be reinterpreted", "contents": "The \"hidden ligand\" of the galactose-binding protein. Following tryptophan fluorescence of the galactose-binding during dissociation of the ligand it has been found that glucose dissociates with a half life of less than 5 s. Similarly, fast dissociation was also observed by following release of radioactively labelled glucose from Sepharose-coupled galactose-binding protein upon dilution. Accordingly, a previous claim that the galactose-binding protein contains glucose as a non-dissociable \"hidden ligand\" [G. Richarme and A. Kepes (1974) Eur. J. Biochem. 45, 127-133] has to be reinterpreted"}
{"id": "PMID:1097248", "title": "Purification and characterization of a new factor which restores protein synthesis in  a conditionally lethal mutant of Escherichia coli.", "content": "The mutant Escherichia coli strain, N4316, has a temperature-sensitive defect in a protein factor required for translation in vitro of bacteriophage f2 RNA. We have purified the normal counterpart of this factor from a wild-type strain, using as an assay its ability to restore the activity of mutant extracts at non-permissive temperature. Our final preparation is free of known initiation, propagation, and release factors, proving that the factor is a new component required for translation. The new factor has a molecular weight of 95000 with preliminary data suggesting a subunit structure. 70% of this protein is found in the soluble-cell fraction, the rest being associated with 70-S ribosomes. Kinetic analyses indicate that the factor acts early in translation. Expression of the defect is highly dependent on the Mg2+ concentration, no temperature-sensitivity being apparent at 15 mM Mg2+. At lower Mg2+ concentrations, the defect is expressed only with natural mRNAs such as f2 RNA, and not with artifical polymers such as poly(U). This specificity suggests that the factor may function in events coded by special sequences in the natural messengers", "contents": "Purification and characterization of a new factor which restores protein synthesis in  a conditionally lethal mutant of Escherichia coli. The mutant Escherichia coli strain, N4316, has a temperature-sensitive defect in a protein factor required for translation in vitro of bacteriophage f2 RNA. We have purified the normal counterpart of this factor from a wild-type strain, using as an assay its ability to restore the activity of mutant extracts at non-permissive temperature. Our final preparation is free of known initiation, propagation, and release factors, proving that the factor is a new component required for translation. The new factor has a molecular weight of 95000 with preliminary data suggesting a subunit structure. 70% of this protein is found in the soluble-cell fraction, the rest being associated with 70-S ribosomes. Kinetic analyses indicate that the factor acts early in translation. Expression of the defect is highly dependent on the Mg2+ concentration, no temperature-sensitivity being apparent at 15 mM Mg2+. At lower Mg2+ concentrations, the defect is expressed only with natural mRNAs such as f2 RNA, and not with artifical polymers such as poly(U). This specificity suggests that the factor may function in events coded by special sequences in the natural messengers"}
{"id": "PMID:1097249", "title": "Topology of binding sites for carbamyl phosphate in aspartate transcarbamylase from Escherichia coli. The use of pyridoxal phosphate as covalent probe.", "content": "Pyridoxal phosphate, a competitive inhibitor of aspartate transcarbamylase, binds to six sites in the catalytic and to twelve sites in the regulatory subunits of this hexameric protein. The properties of its association to the active sites of the enzyme are very similar to those observed with one of its substrates, carbamyl phosphate. It tightly binds to one half of the sites in the absence of succinate, an analogue of the second substrate. Since pyridoxal phosphate can be linked covalently to the protein by reduction, the distribution of the high affinity binding sites on catalytic trimers was studied after dissociation of modified holoenzyme. Electrophoresis of isolated subunits under non-denaturing conditions revealed four distinct bands, corresponding to trimers containing 0 to 3 pyridoxal phosphate derivatives. The distribution among the four species as a function of ligand concentration in the absence of succinate indicates that in the native oligomer, pyridoxal phosphate (and by extrapolation, carbamyl phosphate) binds to both catalytic trimers, rather than to three sites on a single subunit.", "contents": "Topology of binding sites for carbamyl phosphate in aspartate transcarbamylase from Escherichia coli. The use of pyridoxal phosphate as covalent probe. Pyridoxal phosphate, a competitive inhibitor of aspartate transcarbamylase, binds to six sites in the catalytic and to twelve sites in the regulatory subunits of this hexameric protein. The properties of its association to the active sites of the enzyme are very similar to those observed with one of its substrates, carbamyl phosphate. It tightly binds to one half of the sites in the absence of succinate, an analogue of the second substrate. Since pyridoxal phosphate can be linked covalently to the protein by reduction, the distribution of the high affinity binding sites on catalytic trimers was studied after dissociation of modified holoenzyme. Electrophoresis of isolated subunits under non-denaturing conditions revealed four distinct bands, corresponding to trimers containing 0 to 3 pyridoxal phosphate derivatives. The distribution among the four species as a function of ligand concentration in the absence of succinate indicates that in the native oligomer, pyridoxal phosphate (and by extrapolation, carbamyl phosphate) binds to both catalytic trimers, rather than to three sites on a single subunit."}
{"id": "PMID:1097250", "title": "Digestion with matrix-bound proteases as a possible probe for the topography of the DNA-dependent RNA polymerase from Escherichia coli.", "content": "DNA-dependent RNA polymerase lacking subunit sigma was digested with matrix-bound chymotrypsin or trypsin in the presence of 0.4 M NaCl in the monomeric form or at low ionic strength in the oligomeric form. Sigma-containing polymerase was digested in the same way. The course of proteolysis was followed by polyacrylamide gel electrophoresis after dissociation of the enzyme with detergent into subunits and the fragments produced by the hydrolysis. The following results were obtained. (a) The large subunits beta and beta' are cleaved with a much higher rate in the monomeric than in the oligomeric polymerase. (b) Both large subunits are hydrolysed with the same rate. (c) Subunit alpha is hydrolysed almost with the same rate in the monomeric and oligomeric form of polymerase. (d) The same was found for subunit sigma. (e) These effects were independent of the substrate specificity of the protease used. (f) Subunit sigma is much more susceptible to chymotrypsin than to trypsin. (g) Subunit sigma protects the large subunits beta and beta' against tryptic cleavage. These results can be explained in terms of a tentative model for the topography of the protomer-protomer interactions in RNA polymerase. According to this model subunits beta and beta' contain two sites for isologous interactions of protomers. One site can be blocked by attachment of subunit sigma. Subunits alpha and sigma do not participate directly in the association.", "contents": "Digestion with matrix-bound proteases as a possible probe for the topography of the DNA-dependent RNA polymerase from Escherichia coli. DNA-dependent RNA polymerase lacking subunit sigma was digested with matrix-bound chymotrypsin or trypsin in the presence of 0.4 M NaCl in the monomeric form or at low ionic strength in the oligomeric form. Sigma-containing polymerase was digested in the same way. The course of proteolysis was followed by polyacrylamide gel electrophoresis after dissociation of the enzyme with detergent into subunits and the fragments produced by the hydrolysis. The following results were obtained. (a) The large subunits beta and beta' are cleaved with a much higher rate in the monomeric than in the oligomeric polymerase. (b) Both large subunits are hydrolysed with the same rate. (c) Subunit alpha is hydrolysed almost with the same rate in the monomeric and oligomeric form of polymerase. (d) The same was found for subunit sigma. (e) These effects were independent of the substrate specificity of the protease used. (f) Subunit sigma is much more susceptible to chymotrypsin than to trypsin. (g) Subunit sigma protects the large subunits beta and beta' against tryptic cleavage. These results can be explained in terms of a tentative model for the topography of the protomer-protomer interactions in RNA polymerase. According to this model subunits beta and beta' contain two sites for isologous interactions of protomers. One site can be blocked by attachment of subunit sigma. Subunits alpha and sigma do not participate directly in the association."}
{"id": "PMID:1097251", "title": "Metabolism of the phosphatidylglycerol molecular species in Escherichia coli.", "content": "The fractionation, turnover and biosynthesis of the phosphatidylglycerol molecular species of Escherichia coli were studied. Monoacetyldiglycerides derived from phosphatidylglycerol were separated into five subfractions; cis-vaccenyl-palmitoleyl, cis-vaccenyl-cis-vaccenyl, palmityl-palmitoleyl, palmityl-cis-vaccenyl and the disaturated molecular species on a silica gel plate impregnated with silver nitrate. Individual molecular species had different turnover rates. The palmityl-cis-vaccenyl species was metabolized faster than the others. Disaturated species were rather stable. Various phosphatidylglycerol molecular species were synthesized in the presence of sn-glycerol 3-phosphate, palmityl-CoA, palmitoleyl-CoA, cis-vaccenyl-CoA and CTP by the E. coli membrane particulate fraction. When only the proportion of palmityl-CoA among the three acyl-CoAs was increased, the molecular species containing the palmityl residue were increased. Similar results were obtained with the other acyl-CoAs. However, a temperature-sensitive incorporation of unsaturated and saturated fatty acids into phosphatidylglycerol molecular species was observed with no change in the proportions of the three acyl-CoAs, completely reflecting the in vivo unsaturated/saturated ratio.", "contents": "Metabolism of the phosphatidylglycerol molecular species in Escherichia coli. The fractionation, turnover and biosynthesis of the phosphatidylglycerol molecular species of Escherichia coli were studied. Monoacetyldiglycerides derived from phosphatidylglycerol were separated into five subfractions; cis-vaccenyl-palmitoleyl, cis-vaccenyl-cis-vaccenyl, palmityl-palmitoleyl, palmityl-cis-vaccenyl and the disaturated molecular species on a silica gel plate impregnated with silver nitrate. Individual molecular species had different turnover rates. The palmityl-cis-vaccenyl species was metabolized faster than the others. Disaturated species were rather stable. Various phosphatidylglycerol molecular species were synthesized in the presence of sn-glycerol 3-phosphate, palmityl-CoA, palmitoleyl-CoA, cis-vaccenyl-CoA and CTP by the E. coli membrane particulate fraction. When only the proportion of palmityl-CoA among the three acyl-CoAs was increased, the molecular species containing the palmityl residue were increased. Similar results were obtained with the other acyl-CoAs. However, a temperature-sensitive incorporation of unsaturated and saturated fatty acids into phosphatidylglycerol molecular species was observed with no change in the proportions of the three acyl-CoAs, completely reflecting the in vivo unsaturated/saturated ratio."}
{"id": "PMID:1097252", "title": "No correlation between native and denatured forms of tRNA(Trp) form Escherichia coli and the resistant and sensitive molecules characterised by phosphorolysis.  Two classes of conformation characterised by phosphorolysis in both native and denatured tRNA(Trp).", "content": "Some tRNA molecules in solution are sensitive to attack by polynucleotide phosphorylase while others are resistant, even with pure species of tRNA. Further analysis of this behaviour has revealed an underlying microheterogeneity in tRNA structure. In order to clarify the relation between the sensitive and resistant classes of tRNA, and the native and denatured forms with respect to amino acid acceptance, the phosphorolysis of tRNATrp from Escherichia coli has been investigated. Native tRNATrp is similar to species examined previously: resistant and sensitive classes are observed and the sensitive proportion increases with temperature. At 20 degrees C both native and denatured tRNATrp are stable under phosphorolysis conditions, and denaturated tRNATrp is found also to possess resistant and sensitive classes. About 10% of both native and denatured tRNATrp is rapidly phosphorolysed at 20 degrees C, but the rate of conversion of resistant denatured tRNATrp to the sensitive class is about twice as fact as for the native form. Thus it can be concluded that the sensitive molecules of tRNATrp attacked by polynucleotide phosphorylase are not due to denaturation.", "contents": "No correlation between native and denatured forms of tRNA(Trp) form Escherichia coli and the resistant and sensitive molecules characterised by phosphorolysis.  Two classes of conformation characterised by phosphorolysis in both native and denatured tRNA(Trp). Some tRNA molecules in solution are sensitive to attack by polynucleotide phosphorylase while others are resistant, even with pure species of tRNA. Further analysis of this behaviour has revealed an underlying microheterogeneity in tRNA structure. In order to clarify the relation between the sensitive and resistant classes of tRNA, and the native and denatured forms with respect to amino acid acceptance, the phosphorolysis of tRNATrp from Escherichia coli has been investigated. Native tRNATrp is similar to species examined previously: resistant and sensitive classes are observed and the sensitive proportion increases with temperature. At 20 degrees C both native and denatured tRNATrp are stable under phosphorolysis conditions, and denaturated tRNATrp is found also to possess resistant and sensitive classes. About 10% of both native and denatured tRNATrp is rapidly phosphorolysed at 20 degrees C, but the rate of conversion of resistant denatured tRNATrp to the sensitive class is about twice as fact as for the native form. Thus it can be concluded that the sensitive molecules of tRNATrp attacked by polynucleotide phosphorylase are not due to denaturation."}
{"id": "PMID:1097253", "title": "Cerebral aspergillosis with special reference to cerebrospinal fluid findings.", "content": "A case of cerebral aspergillosis in a 69-year-old diabetic man is reported. The disease, initially presenting as headache and vertigo, was caused by Aspergillus fumigatus. Seven CSF specimens were examined. The main findings included decreased glucose and increased protein concentrations, leukocyte reaction and one positive culture for A. fumigatus. Neuropathological examination revealed granulomatous leptomeningitis and typical fungal hyphae. The process probably lasted for more than 1 year and was an important contributing factor to two brain stem infarcts.", "contents": "Cerebral aspergillosis with special reference to cerebrospinal fluid findings. A case of cerebral aspergillosis in a 69-year-old diabetic man is reported. The disease, initially presenting as headache and vertigo, was caused by Aspergillus fumigatus. Seven CSF specimens were examined. The main findings included decreased glucose and increased protein concentrations, leukocyte reaction and one positive culture for A. fumigatus. Neuropathological examination revealed granulomatous leptomeningitis and typical fungal hyphae. The process probably lasted for more than 1 year and was an important contributing factor to two brain stem infarcts."}
{"id": "PMID:1097254", "title": "Association between myasthenia gravis and malignant lymphoma.", "content": "A reticulum cell sarcoma was diagnosed in a myasthenic patient 14 years after the first manifestations of myasthenia gravis. The association of myasthenia and malignancy, especially lymphoreticular, is discussed in view of an increasing number of similar reports in the literature. It is suggested that autoimmune diseases, myasthenia gravis and malignant processes might have a common underlying abnormal immune state related to thymic pathology.", "contents": "Association between myasthenia gravis and malignant lymphoma. A reticulum cell sarcoma was diagnosed in a myasthenic patient 14 years after the first manifestations of myasthenia gravis. The association of myasthenia and malignancy, especially lymphoreticular, is discussed in view of an increasing number of similar reports in the literature. It is suggested that autoimmune diseases, myasthenia gravis and malignant processes might have a common underlying abnormal immune state related to thymic pathology."}
{"id": "PMID:1097255", "title": "Kuru-plaques in creutzfeldt-jakob disease.", "content": "A case of Creutzfeldt-Jakob disease of cerebellar type is reported. In this case the cerebellar cortex exhibited classical Kuru-plaques. From a comparison of this finding with 50 cerebella from senile patients and patients with cerebellar atrophies it is concluded that the Kuru-plaque is specific for Creutzfeldt-Jakob disease, possible even for its cerebellar variant.", "contents": "Kuru-plaques in creutzfeldt-jakob disease. A case of Creutzfeldt-Jakob disease of cerebellar type is reported. In this case the cerebellar cortex exhibited classical Kuru-plaques. From a comparison of this finding with 50 cerebella from senile patients and patients with cerebellar atrophies it is concluded that the Kuru-plaque is specific for Creutzfeldt-Jakob disease, possible even for its cerebellar variant."}
{"id": "PMID:1097256", "title": "Facilitation through hyperventilation of therapeutic effect of pyrithioxin in cerebral infarct patients.", "content": "A method of treatment consisting in administration of a neurodynamic drug, pyrithioxin, combined with a short period of hyperventilation (HV) was applied in cerebral infarct patients with hemiplegia. The combination was superior to pyrithioxin alone for the motor recovery of these patients. In some cases, it induced immediately a partial restoration of motility. The combination constantly brought about an increase in urinary excretion of norepinephrine and vanilmandelic acid, which failed to occur after pyrithioxin or HV alone. HV appears to facilitate the neural effects of the drug by promoting its transfer into the CNS.", "contents": "Facilitation through hyperventilation of therapeutic effect of pyrithioxin in cerebral infarct patients. A method of treatment consisting in administration of a neurodynamic drug, pyrithioxin, combined with a short period of hyperventilation (HV) was applied in cerebral infarct patients with hemiplegia. The combination was superior to pyrithioxin alone for the motor recovery of these patients. In some cases, it induced immediately a partial restoration of motility. The combination constantly brought about an increase in urinary excretion of norepinephrine and vanilmandelic acid, which failed to occur after pyrithioxin or HV alone. HV appears to facilitate the neural effects of the drug by promoting its transfer into the CNS."}
{"id": "PMID:1097257", "title": "His bundle electrograms in patients with congestive cardiomyopathy.", "content": "His bundle electrograms were obtained at rest and during atrial pacing in 16 patients with congestive cardiomyopathy. Increments in heart rate during atrial pacing were associated with progressive increases in the A-H interval in all patients. Three patients had abnormal conduction (prolonged P-H) proximal to the His bundle; atrial pacing resulted in second degree A-V block (A-H block) in these three patients at paced rates below 130/min; these three patients were on digitalis at the time of the study. Two patients ahd left bundle branch block associated with a prolonged H-V interval suggesting a conduction disturbance in the contralateral bundle system either at or distal to the branching portion of the His bundle. Two patients had H-V prolongation in the presence of a normal QRS duration which suggests delay in the bundle of His. Two patients with normal H-V intervals had marked prolongation of the total intraventricular conduction time (H-S prolongation). The His bundle electrogram uncovered abnormalities in atrioventricular conduction not apparent on the standard electrocardiogram and has allowed expansion of our knowledge regarding the electrophysiologic features of congestive cardiomyopathy.", "contents": "His bundle electrograms in patients with congestive cardiomyopathy. His bundle electrograms were obtained at rest and during atrial pacing in 16 patients with congestive cardiomyopathy. Increments in heart rate during atrial pacing were associated with progressive increases in the A-H interval in all patients. Three patients had abnormal conduction (prolonged P-H) proximal to the His bundle; atrial pacing resulted in second degree A-V block (A-H block) in these three patients at paced rates below 130/min; these three patients were on digitalis at the time of the study. Two patients ahd left bundle branch block associated with a prolonged H-V interval suggesting a conduction disturbance in the contralateral bundle system either at or distal to the branching portion of the His bundle. Two patients had H-V prolongation in the presence of a normal QRS duration which suggests delay in the bundle of His. Two patients with normal H-V intervals had marked prolongation of the total intraventricular conduction time (H-S prolongation). The His bundle electrogram uncovered abnormalities in atrioventricular conduction not apparent on the standard electrocardiogram and has allowed expansion of our knowledge regarding the electrophysiologic features of congestive cardiomyopathy."}
{"id": "PMID:1097258", "title": "A new method for the evaluation of analgesic activity using adjuvant-induced arthritis in the rat.", "content": "A bioassay was developed for the assessment of analgesic activity by using pain resulting from a reproducible pathological condition. The vocalization displayed by rats with adjuvant-induced polyarthritis was defined as an expression of pain, and a decrease of this response was established as specifically demonstrating analgesic activity. This method was capable of detecting the analgesic activity of morphine-like and narcotic antagonist-type analgesics, as well as the activity of the antipyretic analgesics. The relative potencies of known analgesic agents determined with this technique closely approximated the potencies of these analgesics in man. The instrumentation for recording and measuring the vocal response of arthritic rats is described.", "contents": "A new method for the evaluation of analgesic activity using adjuvant-induced arthritis in the rat. A bioassay was developed for the assessment of analgesic activity by using pain resulting from a reproducible pathological condition. The vocalization displayed by rats with adjuvant-induced polyarthritis was defined as an expression of pain, and a decrease of this response was established as specifically demonstrating analgesic activity. This method was capable of detecting the analgesic activity of morphine-like and narcotic antagonist-type analgesics, as well as the activity of the antipyretic analgesics. The relative potencies of known analgesic agents determined with this technique closely approximated the potencies of these analgesics in man. The instrumentation for recording and measuring the vocal response of arthritic rats is described."}
{"id": "PMID:1097261", "title": "Impaired radioprotective capacity and reduced proliferative rate of bone marrow from neonatally thymectomized mice;.", "content": "The colony forming capacity of bone marrow from neonatally thymectomized mice is reduced in comparison with that of normal animalsmin addition to this quantitative change, we observed that the bone marrow of thymectomized animals has a reduced radioprotective effect upon inoculation into lethally irradiated recipient mice. It was also found that the cellularity of spleen colonies derived from bone marrow of thymectomized animals is lower than that of intact controls. In vitro uptake of 3H-thymidine into cells of spleen colonies, and rate of DNA synthesis measured in vitro were found to be reduced in cells derived from bone marrow of thymectomized donors; The initially observed reduction in colony forming capactiy of bone marrow from neonatally thymectomized mice could be reversed by thymus reimplantation=", "contents": "Impaired radioprotective capacity and reduced proliferative rate of bone marrow from neonatally thymectomized mice;. The colony forming capacity of bone marrow from neonatally thymectomized mice is reduced in comparison with that of normal animalsmin addition to this quantitative change, we observed that the bone marrow of thymectomized animals has a reduced radioprotective effect upon inoculation into lethally irradiated recipient mice. It was also found that the cellularity of spleen colonies derived from bone marrow of thymectomized animals is lower than that of intact controls. In vitro uptake of 3H-thymidine into cells of spleen colonies, and rate of DNA synthesis measured in vitro were found to be reduced in cells derived from bone marrow of thymectomized donors; The initially observed reduction in colony forming capactiy of bone marrow from neonatally thymectomized mice could be reversed by thymus reimplantation="}
{"id": "PMID:1097260", "title": "alpha-Methylnoradrenaline induced hypotension and bradycardia after administration into the area of the nucleus tractus solitarii.", "content": "Bilateral injections of alpha-methylnoradrenaline into the area of the nucleus tractus solitarii of the brain stem caused a dose-dependent decrease of systemic arterial blood pressure and heart rate of anesthetized rats. The effects were prevented and even reversed by a preceding injection of the alpha-adrenoceptor blocking agent phentolamine. Pressor doses of angiotensin II and of arginine-vasopressin at the same site failed to drcrease blood pressure and heart rate.", "contents": "alpha-Methylnoradrenaline induced hypotension and bradycardia after administration into the area of the nucleus tractus solitarii. Bilateral injections of alpha-methylnoradrenaline into the area of the nucleus tractus solitarii of the brain stem caused a dose-dependent decrease of systemic arterial blood pressure and heart rate of anesthetized rats. The effects were prevented and even reversed by a preceding injection of the alpha-adrenoceptor blocking agent phentolamine. Pressor doses of angiotensin II and of arginine-vasopressin at the same site failed to drcrease blood pressure and heart rate."}
{"id": "PMID:1097266", "title": "Behavioral functions of narcotic antagonists: response-drug contingencies.", "content": "Behavioral effects of the narcotic antagonist naloxone are discussed in terms of stimulus functions. As an eliciting stimulus, the effects of naloxone depend on prior administration of narcotic. Administered independently of responding, naloxone can increase or decrease rates of narcotic-reinforced responding depending on the dose of naloxone. When naloxone is administered as a consequence of narcotic self-injection, the further probability of that behavior is reduced; thus, naloxone can function as a punishing stimulus. As a negatively-reinforcing stimulus, naloxone can maintain behavior which terminates or prevents delivery in morphine-dependent monkeys. In animals with previous naloxone avoidance-escape experience, unavoidable-inescapable injection of naloxone produce increases in avoidance-escape response rates. In these animals, responding subsequently can be maintained, at least temporarily, when naloxone is administered only as the consequence of responding.", "contents": "Behavioral functions of narcotic antagonists: response-drug contingencies. Behavioral effects of the narcotic antagonist naloxone are discussed in terms of stimulus functions. As an eliciting stimulus, the effects of naloxone depend on prior administration of narcotic. Administered independently of responding, naloxone can increase or decrease rates of narcotic-reinforced responding depending on the dose of naloxone. When naloxone is administered as a consequence of narcotic self-injection, the further probability of that behavior is reduced; thus, naloxone can function as a punishing stimulus. As a negatively-reinforcing stimulus, naloxone can maintain behavior which terminates or prevents delivery in morphine-dependent monkeys. In animals with previous naloxone avoidance-escape experience, unavoidable-inescapable injection of naloxone produce increases in avoidance-escape response rates. In these animals, responding subsequently can be maintained, at least temporarily, when naloxone is administered only as the consequence of responding."}
{"id": "PMID:1097259", "title": "Effect of methyldopa treatment on peripheral sympathetic nerve function in the dog.", "content": "Administration of methyldopa (100 mg/kg, p.o. twice daily for 3 days) to mongrel dogs produced a significant decrease in the mean blood pressure (MBP) and heart rate (HR). Chronotropic responses to cardioaccelerator nerve stimulation and vasoconstrictor responses to lumbar sympathetic nerve stimulation were significantly attenuated in the treated dogs. Pressor and chronotropic responses to bilateral carotid occlusion (BCO) and tyramine were also markedly reduced following treatment with methyldopa, which is consistent with the clinical findings that chronic methyldopa treatment in hypertensive patients impairs cardiovascular reflexes. Methylnorepinephrine (MNE) was significantly less potent than norepinephrine (NE) in producing an increase in MBP or hindleg perfusion pressure, while the chronotropic and inotropic potency of MNE was about equal to NE. These results indicate that treatment with methyldopa in the dog does indeed produce an impairment of peripheral sympathetic neuronal function to certain organs and this peripheral effect of the drug may be of importance in accounting for its antihypertensive action in the dog.", "contents": "Effect of methyldopa treatment on peripheral sympathetic nerve function in the dog. Administration of methyldopa (100 mg/kg, p.o. twice daily for 3 days) to mongrel dogs produced a significant decrease in the mean blood pressure (MBP) and heart rate (HR). Chronotropic responses to cardioaccelerator nerve stimulation and vasoconstrictor responses to lumbar sympathetic nerve stimulation were significantly attenuated in the treated dogs. Pressor and chronotropic responses to bilateral carotid occlusion (BCO) and tyramine were also markedly reduced following treatment with methyldopa, which is consistent with the clinical findings that chronic methyldopa treatment in hypertensive patients impairs cardiovascular reflexes. Methylnorepinephrine (MNE) was significantly less potent than norepinephrine (NE) in producing an increase in MBP or hindleg perfusion pressure, while the chronotropic and inotropic potency of MNE was about equal to NE. These results indicate that treatment with methyldopa in the dog does indeed produce an impairment of peripheral sympathetic neuronal function to certain organs and this peripheral effect of the drug may be of importance in accounting for its antihypertensive action in the dog."}
{"id": "PMID:1097267", "title": "Catecholamines and drug-behavior interactions.", "content": "The effects of several drugs on schedule-controlled operant behavior depend on the baseline rate of responding and on the nature of the environmental conditions that maintain the behavior. For example, the effects of amphetamine and alpha-methylpara-tyrosine (alphaMT) on operant performances depend to a large extent on the rate at which organisms respond under nondrug control conditions. A neurochemical mechanism for these rate-dependent effects has not been established. However, several lines of evidence suggest that catecholamines are functionally important in the maintenance of many types of behavior, including operant behavior. The fact that many drugs which exhibit drug-behavior interactions also produce characteristic effects on the metabolism of central nervous system catecholamines suggests that the performance of operant behavior per se modifies brain catecholamine metabolism and thereby the subsequent drug effect. Experiments measuring the depletion of catecholamines following synthesis inhibition with alphaMT, or changes in the specific activity of norepinephrine after tritium labeling, have shown that operant behavior alters the metabolism of catecholamines. Preliminary evidence  is also presented from experiments designed to determine variables associated with the performance-induced changes in catecholamine metabolism. These variables include: rate of responses; rate or density of reinforcement; and response-reinforcer contingencies. The results of these experiments suggest a neurochemical mechanism for the rate-dependent effects of amphetamine and alphaMT. A model is presented that may account for the general phenomenon of drug-behavior interactions in neurochemical terms.", "contents": "Catecholamines and drug-behavior interactions. The effects of several drugs on schedule-controlled operant behavior depend on the baseline rate of responding and on the nature of the environmental conditions that maintain the behavior. For example, the effects of amphetamine and alpha-methylpara-tyrosine (alphaMT) on operant performances depend to a large extent on the rate at which organisms respond under nondrug control conditions. A neurochemical mechanism for these rate-dependent effects has not been established. However, several lines of evidence suggest that catecholamines are functionally important in the maintenance of many types of behavior, including operant behavior. The fact that many drugs which exhibit drug-behavior interactions also produce characteristic effects on the metabolism of central nervous system catecholamines suggests that the performance of operant behavior per se modifies brain catecholamine metabolism and thereby the subsequent drug effect. Experiments measuring the depletion of catecholamines following synthesis inhibition with alphaMT, or changes in the specific activity of norepinephrine after tritium labeling, have shown that operant behavior alters the metabolism of catecholamines. Preliminary evidence  is also presented from experiments designed to determine variables associated with the performance-induced changes in catecholamine metabolism. These variables include: rate of responses; rate or density of reinforcement; and response-reinforcer contingencies. The results of these experiments suggest a neurochemical mechanism for the rate-dependent effects of amphetamine and alphaMT. A model is presented that may account for the general phenomenon of drug-behavior interactions in neurochemical terms."}
{"id": "PMID:1097268", "title": "Assessing the impact of low level chemicals on development: behavioral and latent effects.", "content": "There is growing evidence that nervous tissue, especially the brain, is more sensitive to many foreign chemical substances than has previously been suspected, and that toxic effects may be manifested as subtle disturbances of behavior long before any classical symptoms of poisoning become apparent. Early detection of an insidious toxic process (behavioral toxicology) may enable the prevention or attenuation of harm to humans and other organisms. Adding to both the sensitivity and complexity of behavioral toxicologic testing is the increasing evidence that individuals are more vulnerable to adverse factors during the period of development (conception yields puberty) than at any other time in life. Subtle functional disturbances in organisms exposed while immature (behavioral teratology) may be one of the most sensitive indicators of chemical toxicity. Furthermore, defects in a developmental process may have only delayed effects. A morphological or biochemical lesion can be dormant and not manifest itself until later in life as a behavioral disorder, mental deficiency, or overt functional impairment. Longitudinal evaluation is required to detect long-term or delayed effects of a particular developmental influence on biological and behavioral functions. Examples from research on the subtle and latent consequences of prenatal and early postnatal exposure to methylmercury that illustrate the above principles are presented. It is concluded that behavioral and long-term evaluation of organisms exposed during development are essential for a thorough assessment of the impact of certain low level chemicals on human health.", "contents": "Assessing the impact of low level chemicals on development: behavioral and latent effects. There is growing evidence that nervous tissue, especially the brain, is more sensitive to many foreign chemical substances than has previously been suspected, and that toxic effects may be manifested as subtle disturbances of behavior long before any classical symptoms of poisoning become apparent. Early detection of an insidious toxic process (behavioral toxicology) may enable the prevention or attenuation of harm to humans and other organisms. Adding to both the sensitivity and complexity of behavioral toxicologic testing is the increasing evidence that individuals are more vulnerable to adverse factors during the period of development (conception yields puberty) than at any other time in life. Subtle functional disturbances in organisms exposed while immature (behavioral teratology) may be one of the most sensitive indicators of chemical toxicity. Furthermore, defects in a developmental process may have only delayed effects. A morphological or biochemical lesion can be dormant and not manifest itself until later in life as a behavioral disorder, mental deficiency, or overt functional impairment. Longitudinal evaluation is required to detect long-term or delayed effects of a particular developmental influence on biological and behavioral functions. Examples from research on the subtle and latent consequences of prenatal and early postnatal exposure to methylmercury that illustrate the above principles are presented. It is concluded that behavioral and long-term evaluation of organisms exposed during development are essential for a thorough assessment of the impact of certain low level chemicals on human health."}
{"id": "PMID:1097270", "title": "The role of discriminative stimuli in modulating drug action.", "content": "Behavior reinforced in the presence of a stimulus comes under the control of the stimulus. A drug can then modify that control and, therefore, modify the behavior itself. Studies over the past 2 decades have shown that the nature of the controlling (or discriminative) stimulus can govern the degree to which drugs change performance. These experiments usually have compared behavior on various schedules of reinforcement with and without added discriminative stimuli. For instance, pigeons that had been trained on a fixed-interval schedule showed great changes in response distribution after amphetamine and scopolamine. The same birds, when performing on a fixed-interval schedule to which time-correlated discriminative stimuli had been added, showed smaller changes in response distribution. Other pigeons were trained to make a minimum number of consecutive responses on one key before a peck on a second key would be reinforced; beta-amphetamine and scopolamine led to pronounced increases in premature switching. Adding a discriminative stimulus when the response requirement was fulfilled increased the likelihood that a switch would occur only after the appropriate number of pecks had been emitted. It also attenuated the effects of the drugs. The presence of discriminative stimuli did not make as large a difference in performance in either of these experiments when chlorpromazine and promazine were studied. In general, work with other schedules of reinforcement supports the conclusion that behavior under stron external stimulus control is less apt to be readily affected by many drugs. Addition of the discriminative stimulus can also \"improve\" the behavior of pigeons that have been given enough methylmercury to increase greatly the variability of their performance.", "contents": "The role of discriminative stimuli in modulating drug action. Behavior reinforced in the presence of a stimulus comes under the control of the stimulus. A drug can then modify that control and, therefore, modify the behavior itself. Studies over the past 2 decades have shown that the nature of the controlling (or discriminative) stimulus can govern the degree to which drugs change performance. These experiments usually have compared behavior on various schedules of reinforcement with and without added discriminative stimuli. For instance, pigeons that had been trained on a fixed-interval schedule showed great changes in response distribution after amphetamine and scopolamine. The same birds, when performing on a fixed-interval schedule to which time-correlated discriminative stimuli had been added, showed smaller changes in response distribution. Other pigeons were trained to make a minimum number of consecutive responses on one key before a peck on a second key would be reinforced; beta-amphetamine and scopolamine led to pronounced increases in premature switching. Adding a discriminative stimulus when the response requirement was fulfilled increased the likelihood that a switch would occur only after the appropriate number of pecks had been emitted. It also attenuated the effects of the drugs. The presence of discriminative stimuli did not make as large a difference in performance in either of these experiments when chlorpromazine and promazine were studied. In general, work with other schedules of reinforcement supports the conclusion that behavior under stron external stimulus control is less apt to be readily affected by many drugs. Addition of the discriminative stimulus can also \"improve\" the behavior of pigeons that have been given enough methylmercury to increase greatly the variability of their performance."}
{"id": "PMID:1097271", "title": "Inhibition of implantation in the golden hamster by zona-precipitating antibody.", "content": "Antisera prepared against saline extracts of hamster ovary, and made specific to ovary by absorption with small intestine, precipitated the zona pellucida of provulatory hamster ova and all early embryonic stages examined, including the pronuclear, 2-, 4-, 8-, and 16-cell, morula, and blastocyst stages; this indicates that (1) the zona antigen is present during oocyte maturation and early embryonic development and (2) the antigen does not change its ability to react with zonaprecipitating antibody. Embryos in the morula and blastocyst stages, treated in vitro with zona-precipitating antibody, pre-immunization rabbit serum, or control media were transferred into synchronized pseudopregnant recipients. Precipitation of zonae by specific antibody inhibited implantation by blocking the process known as zona shedding, and thereby prevented the embryo from making contact with the uterus.", "contents": "Inhibition of implantation in the golden hamster by zona-precipitating antibody. Antisera prepared against saline extracts of hamster ovary, and made specific to ovary by absorption with small intestine, precipitated the zona pellucida of provulatory hamster ova and all early embryonic stages examined, including the pronuclear, 2-, 4-, 8-, and 16-cell, morula, and blastocyst stages; this indicates that (1) the zona antigen is present during oocyte maturation and early embryonic development and (2) the antigen does not change its ability to react with zonaprecipitating antibody. Embryos in the morula and blastocyst stages, treated in vitro with zona-precipitating antibody, pre-immunization rabbit serum, or control media were transferred into synchronized pseudopregnant recipients. Precipitation of zonae by specific antibody inhibited implantation by blocking the process known as zona shedding, and thereby prevented the embryo from making contact with the uterus."}
{"id": "PMID:1097272", "title": "Evaluation of human sera for antibodies against sperm by immunofluorescence.", "content": "Newborn cord sera and sera from pregnant women and from couples with unexplained infertility were observed by indirect immunofluorescence for reactions against human sperm. The sera from the infertile couples had been tested for macro-agglutination and complement-dependent immobilizing antibodies as well. Nonspecific fluorescence was noted only on the quatorial segment. Acrosomes and tail end-pieces were strongly immunofluorescent (positive at a serum dilution of 1:16) with 60 to 70% and 30 to 50%, respectively, of the sera from each main group, with the exception of the nonreactive cord blood. End-piece fluorescence was primarily due to the IgM globulin fraction. It is suggested that cord serum anti-sperm antibodies, directed mainly against the tail main piece, are due to IgG passively acquired from the mother. There was no apparent difference in immunofluorescence between infertile patients and control groups (except for the acrosome and end-piece, with cord sera). However, when the sera from infertile patients were subdivided into groups containing only agglutinating antibody, only immobilizing antibody, and both types of antibody, a higher percentage of the sera from the group having only agglutinating antibody reacted with all sperm areas. This reaction seemed more uniform over the sperm and included the usually negative posnuclear cap and midpiece. The other subgroups showed no obvious trends.", "contents": "Evaluation of human sera for antibodies against sperm by immunofluorescence. Newborn cord sera and sera from pregnant women and from couples with unexplained infertility were observed by indirect immunofluorescence for reactions against human sperm. The sera from the infertile couples had been tested for macro-agglutination and complement-dependent immobilizing antibodies as well. Nonspecific fluorescence was noted only on the quatorial segment. Acrosomes and tail end-pieces were strongly immunofluorescent (positive at a serum dilution of 1:16) with 60 to 70% and 30 to 50%, respectively, of the sera from each main group, with the exception of the nonreactive cord blood. End-piece fluorescence was primarily due to the IgM globulin fraction. It is suggested that cord serum anti-sperm antibodies, directed mainly against the tail main piece, are due to IgG passively acquired from the mother. There was no apparent difference in immunofluorescence between infertile patients and control groups (except for the acrosome and end-piece, with cord sera). However, when the sera from infertile patients were subdivided into groups containing only agglutinating antibody, only immobilizing antibody, and both types of antibody, a higher percentage of the sera from the group having only agglutinating antibody reacted with all sperm areas. This reaction seemed more uniform over the sperm and included the usually negative posnuclear cap and midpiece. The other subgroups showed no obvious trends."}
{"id": "PMID:1097276", "title": "Basement membranes: current concepts of structure and synthesis.", "content": "Basement membranes are composed of dissimilar protein subunits. The procollagen-like subunit is associated with noncollagenous matrix glycoproteins. The proportion of the latter components varies among basement membranes. The various subunits interact via hydrogen bonds, disulfide bonds and aldehyde-derived cross-links. The extensive degree of cross-linking renders basement membranes highly insoluble. A procollagen-like molecule, extracted from calf anterior lens capsule, exhibits on electron microscopy a filamentous component with a globular portion attached at one end. Treatment of basement membranes with pepsin at low temperature digests the noncollagenous glycoprotein components and allows the collagenous component to come into solution. Purification of the pepsin-solubilized collagen from basement membranes reveals a molecule composed of three identical alpha-chains. Other unique features include 40-50 residues of hydroxylysine, 128-140 residues of 4-hydroxyproline, 12-15 residues of 3-hydroxyproline, 29 residues of arginine, 35 residues alanine, 2-4 residues of half-cystine, 38 residues of glucosyl-galactosyl-hydroxylysine, 3 residues of mannose, 2 residues of glucosamine, and 0.3 residues of fucose. Immunochemical studies indicate the presence of three distinct antigenic components and support the evidence that one is collagenous and the other two are noncollagenous glycoproteins. One of the latter corresponds to the non-helical extension of procollagen. The other is a large-molecular weight highly cross-linked matrix protein.", "contents": "Basement membranes: current concepts of structure and synthesis. Basement membranes are composed of dissimilar protein subunits. The procollagen-like subunit is associated with noncollagenous matrix glycoproteins. The proportion of the latter components varies among basement membranes. The various subunits interact via hydrogen bonds, disulfide bonds and aldehyde-derived cross-links. The extensive degree of cross-linking renders basement membranes highly insoluble. A procollagen-like molecule, extracted from calf anterior lens capsule, exhibits on electron microscopy a filamentous component with a globular portion attached at one end. Treatment of basement membranes with pepsin at low temperature digests the noncollagenous glycoprotein components and allows the collagenous component to come into solution. Purification of the pepsin-solubilized collagen from basement membranes reveals a molecule composed of three identical alpha-chains. Other unique features include 40-50 residues of hydroxylysine, 128-140 residues of 4-hydroxyproline, 12-15 residues of 3-hydroxyproline, 29 residues of arginine, 35 residues alanine, 2-4 residues of half-cystine, 38 residues of glucosyl-galactosyl-hydroxylysine, 3 residues of mannose, 2 residues of glucosamine, and 0.3 residues of fucose. Immunochemical studies indicate the presence of three distinct antigenic components and support the evidence that one is collagenous and the other two are noncollagenous glycoproteins. One of the latter corresponds to the non-helical extension of procollagen. The other is a large-molecular weight highly cross-linked matrix protein."}
{"id": "PMID:1097277", "title": "Changes of pituitary sensitivity to LH-RH during the rat estrous cycle.", "content": "The plasma LH (luteinizing hormone) response to 200 ng of LH-RH (LH-releasing hormone) injected subcutaneously at different stages of the estrous cycle in normal rats under Surital anesthisia was maximal during the afternoon of proestrus and lowest on diestrus I. The area under the plasma LH curve measured at 13:00 on proestrus was approximately 7-fold higher than that obtained at 15:30 h on diestrus I. Intermediate responses were found on diestrus II, estrus and morning of proestrus. An approsimately 2.5-fold higher LH response was observed on proestrus than on diestrus I after injection of [D-Ala2, des Gly-NH2-10] LH-RH ethylamide at 15:00 h. That these marked changes of LH response are not secondary to interference by endogenous LH-RH, changes of the metabolism or transport of exogenous LH-RH or modification of plasma LH clearance is ascertanied by the finding of similar changes of pituitary sensitivity to LH-RH under in vitro conditions using pituitaries collected at the same stag-s of the estrous cycle. As measured both in vivo and in vitro, not only the amplitude but also the speed of LH response are maximal during the afternoon of proestrus and minimal on diestrus I.", "contents": "Changes of pituitary sensitivity to LH-RH during the rat estrous cycle. The plasma LH (luteinizing hormone) response to 200 ng of LH-RH (LH-releasing hormone) injected subcutaneously at different stages of the estrous cycle in normal rats under Surital anesthisia was maximal during the afternoon of proestrus and lowest on diestrus I. The area under the plasma LH curve measured at 13:00 on proestrus was approximately 7-fold higher than that obtained at 15:30 h on diestrus I. Intermediate responses were found on diestrus II, estrus and morning of proestrus. An approsimately 2.5-fold higher LH response was observed on proestrus than on diestrus I after injection of [D-Ala2, des Gly-NH2-10] LH-RH ethylamide at 15:00 h. That these marked changes of LH response are not secondary to interference by endogenous LH-RH, changes of the metabolism or transport of exogenous LH-RH or modification of plasma LH clearance is ascertanied by the finding of similar changes of pituitary sensitivity to LH-RH under in vitro conditions using pituitaries collected at the same stag-s of the estrous cycle. As measured both in vivo and in vitro, not only the amplitude but also the speed of LH response are maximal during the afternoon of proestrus and minimal on diestrus I."}
{"id": "PMID:1097278", "title": "In vitro pituitary responsiveness to gonadotropin-releasing hormone (LH-RH) in intact and castrated male and female rats.", "content": "The in vitro response of pituitaries isolated from both normal and 18-21 day post-castration male and female intact rats to incremental doses of synthetic gonadotropin-releasing hormone (LH-RH) has been investigated. Intact male pituitaries released luteinizing hormone (LH) maximally at the smallest dose of LH-RH (0.1 ng/ml) whereas intact female pituitaries released LH in a dose-response fashion. FSH release from intact male pituitaries was considerably greater than that from intact female pituitaries. As with LH, intact male pituitaries appeared maximally stimulated at 0.1 ng/ml of LH-RH. Intact female pituitaries did not release FSH until a 10 ng/ml dose of LH-RH was used. Male and female castrate pituitaries were more susceptible to LH-RH-induced LH and FSH release than were their intact counterparts, although this was more pronounced with regard to LH release. In addition castrate male pituitaries were more sensitive to lower doses of LH-RH than were castrate female pituitaries, this being most pronounced regarding LH release. Castrate female pituitaries released less FSH at the 100 ng/ml dose than at the 10 ng/ml dose, possibly indicating inhibition at these higher doses. In addition, pituitary extraction and serum from normal and castrate male and female rats were examined for LH and FSH content. LH content of castrated rat pituitaries of both sexes was considerably greater than that of their intact counterparts, as expected. However, castrate male pituitaries contained significantly less FSH than intact male pituitaries, whereas the opposite was true for the female groups. Serum LH and FSH levels were increased in the castrate groups with no difference between sexes. Serum from intact males contained considerably more FSH than did the serum from intact females.", "contents": "In vitro pituitary responsiveness to gonadotropin-releasing hormone (LH-RH) in intact and castrated male and female rats. The in vitro response of pituitaries isolated from both normal and 18-21 day post-castration male and female intact rats to incremental doses of synthetic gonadotropin-releasing hormone (LH-RH) has been investigated. Intact male pituitaries released luteinizing hormone (LH) maximally at the smallest dose of LH-RH (0.1 ng/ml) whereas intact female pituitaries released LH in a dose-response fashion. FSH release from intact male pituitaries was considerably greater than that from intact female pituitaries. As with LH, intact male pituitaries appeared maximally stimulated at 0.1 ng/ml of LH-RH. Intact female pituitaries did not release FSH until a 10 ng/ml dose of LH-RH was used. Male and female castrate pituitaries were more susceptible to LH-RH-induced LH and FSH release than were their intact counterparts, although this was more pronounced with regard to LH release. In addition castrate male pituitaries were more sensitive to lower doses of LH-RH than were castrate female pituitaries, this being most pronounced regarding LH release. Castrate female pituitaries released less FSH at the 100 ng/ml dose than at the 10 ng/ml dose, possibly indicating inhibition at these higher doses. In addition, pituitary extraction and serum from normal and castrate male and female rats were examined for LH and FSH content. LH content of castrated rat pituitaries of both sexes was considerably greater than that of their intact counterparts, as expected. However, castrate male pituitaries contained significantly less FSH than intact male pituitaries, whereas the opposite was true for the female groups. Serum LH and FSH levels were increased in the castrate groups with no difference between sexes. Serum from intact males contained considerably more FSH than did the serum from intact females."}
{"id": "PMID:1097279", "title": "RAST studies : IgE antibodies to Dermatogoides pteronyssinus (house dust mite), Aspergillus fumigatus and beta-lactoglobulin in sudden death in infancy syndrome (SDIS).", "content": "The incidence of 2.5 SDIS cases per 1,000 live births found in Western Australia is in agreement with figures reported for other centres. While the age range of SDIS victims extended from two weeks to 15 months, 57 per cent of deaths occurred in children of two to four months of age. Boys outnumbered girls 1.6:1. Environmental factors are implicated in that the majority of deaths occurred in a biphasic distribution - autumn and late winter months. No significant differences were observed in total IgE levels in serum from SDIS victims, post mortem children who died in trauma of known aetiology and live control children of the same age range. Serum IgE antibodies to D.pteronyssinus were found in 37% of SDIS victims compared with 7% of matched controls (post mortem plus live groups). IgE antibodies to beta-lactoglobulin, the major allergen of cow's milk, appeared with twice the frequency in SDIS vs. control group but both groups showed a similar incidence of antibodies to the allergens of Aspergillus fumigatus. The prevalence of IgE antibodies to D.pteronyssinus in SDIS victims who died in the late winter -- early spring period was double that found in the group who died in the autumn period. Sixtyfour percent of the SDIS victims had antibodies to two or more of the three allergens tested while the control sera were positive to only one allergen. These results support the hypothesis that anaphylaxis induced by immediate hypersensitivity to D.pteronyssinus in particular may be one of the causative factors in SDIS in Western Australia.", "contents": "RAST studies : IgE antibodies to Dermatogoides pteronyssinus (house dust mite), Aspergillus fumigatus and beta-lactoglobulin in sudden death in infancy syndrome (SDIS). The incidence of 2.5 SDIS cases per 1,000 live births found in Western Australia is in agreement with figures reported for other centres. While the age range of SDIS victims extended from two weeks to 15 months, 57 per cent of deaths occurred in children of two to four months of age. Boys outnumbered girls 1.6:1. Environmental factors are implicated in that the majority of deaths occurred in a biphasic distribution - autumn and late winter months. No significant differences were observed in total IgE levels in serum from SDIS victims, post mortem children who died in trauma of known aetiology and live control children of the same age range. Serum IgE antibodies to D.pteronyssinus were found in 37% of SDIS victims compared with 7% of matched controls (post mortem plus live groups). IgE antibodies to beta-lactoglobulin, the major allergen of cow's milk, appeared with twice the frequency in SDIS vs. control group but both groups showed a similar incidence of antibodies to the allergens of Aspergillus fumigatus. The prevalence of IgE antibodies to D.pteronyssinus in SDIS victims who died in the late winter -- early spring period was double that found in the group who died in the autumn period. Sixtyfour percent of the SDIS victims had antibodies to two or more of the three allergens tested while the control sera were positive to only one allergen. These results support the hypothesis that anaphylaxis induced by immediate hypersensitivity to D.pteronyssinus in particular may be one of the causative factors in SDIS in Western Australia."}
{"id": "PMID:1097280", "title": "Multiple mycobacterial antigens in diagnostic tuberculins.", "content": "Guinea-pig antisera containing IgG1 antibodies have been prepared either with killed mycobacterial in paraffin oil or living BCG or intradermal injections of fractions of unheated culture filtrate. Sera, found to contain antibodies specific for different mycobacterial antigens are used in passive cutaneous anaphylaxis (PCA) in 12 times the dilution-to-extinction titer. Animals prepared with sera respond in all sites when unheated culture filtrate, strain H37Rv, is injected intravenously (i.v.) at 17-20 hours. Injection of diagnostic tuberculins i.v. into parallel recipients, in selected amounts, reveals, through reacting sites, the presence of the corresponding antigens and some information on relative amounts. Tuberculins of PPD type are precipitated from heated culture filtrate by ammonium sulfate or trichloroacetic acid (TCA) or benzoic acid. Old Tuberculins are made classically by long heating and evaporation. In all products heat-labile antigens are absent or present in small amount; yet, PPD-S was found to possess five antigens. In all PPD products, there was one dominant antigen, absent from Old Tuberculins. The dominant antigen of OT's was excluded from the TCA-PPD's but was present in most ammonium sulfate-type PPD's. It can be concluded that diagnostic testing made with OT detects a particular delayed-type sensitivity that is different in specificity from that which reacts to TCA-PPD's. Many but not all of the ammonium sulfate PPD's test for both specificities. Several different delayed-type sensitivities are acquired during infection. Supported by Grant AI 01258 of the National Institute of Health.", "contents": "Multiple mycobacterial antigens in diagnostic tuberculins. Guinea-pig antisera containing IgG1 antibodies have been prepared either with killed mycobacterial in paraffin oil or living BCG or intradermal injections of fractions of unheated culture filtrate. Sera, found to contain antibodies specific for different mycobacterial antigens are used in passive cutaneous anaphylaxis (PCA) in 12 times the dilution-to-extinction titer. Animals prepared with sera respond in all sites when unheated culture filtrate, strain H37Rv, is injected intravenously (i.v.) at 17-20 hours. Injection of diagnostic tuberculins i.v. into parallel recipients, in selected amounts, reveals, through reacting sites, the presence of the corresponding antigens and some information on relative amounts. Tuberculins of PPD type are precipitated from heated culture filtrate by ammonium sulfate or trichloroacetic acid (TCA) or benzoic acid. Old Tuberculins are made classically by long heating and evaporation. In all products heat-labile antigens are absent or present in small amount; yet, PPD-S was found to possess five antigens. In all PPD products, there was one dominant antigen, absent from Old Tuberculins. The dominant antigen of OT's was excluded from the TCA-PPD's but was present in most ammonium sulfate-type PPD's. It can be concluded that diagnostic testing made with OT detects a particular delayed-type sensitivity that is different in specificity from that which reacts to TCA-PPD's. Many but not all of the ammonium sulfate PPD's test for both specificities. Several different delayed-type sensitivities are acquired during infection. Supported by Grant AI 01258 of the National Institute of Health."}
{"id": "PMID:1097281", "title": "Physical-chemical properties of precipitated and fractionated allergens from Mycobacterium bovis.", "content": "Seven allergens were prepared from cultures of Mycobacterium bovis. The culture filtrates were divided in half and each aliquot precipitated with either ammonium sulfate or trichloroacetic acid to prepare purified protein derivatives (PPD). These samples of PPD-bovis were analyzed for nitrogen, carbohydrate, RNA and DNA. Physical tests, such as: cellulose acetate electrophoresis, disc gel electrophoresis, ultracentrifugation, isoelectricfocusing and gel filtration were also performed. Precipitation with ammonium sulfate provided PPD's with less impurities than those precipitated with trichloroacetic acid although there was little difference in the allergenic activity of the products precipitated by either method. Several lots of PPD-bovis were fractionated by means of ultrafiltration and the fractions analyzed by many of the above chemical and physical tests as well as tests for amino acid composition and allergenic activity. The greatest amount of protein was found in the fractions with an apparent molecular weight in excess of 300,000. The carbohydrate was usually distributed in proportion to the amount of protein in each fraction while the nucleic acids were predominately found in the higher molecular weight fractions. The amino acid analysis and allergenic activity of the various molecular weight fractions provided data that would support the theory that the various fractions are polymers of a small molecular weight sub-unit. The number of amino acid residues were calculated and the minimum molecular weight of the tubercular protein estimated to be 8700. The estimated molecular weights of the fractions and the amino acid data concur with those previously reported for proteins from other mycobacteria.", "contents": "Physical-chemical properties of precipitated and fractionated allergens from Mycobacterium bovis. Seven allergens were prepared from cultures of Mycobacterium bovis. The culture filtrates were divided in half and each aliquot precipitated with either ammonium sulfate or trichloroacetic acid to prepare purified protein derivatives (PPD). These samples of PPD-bovis were analyzed for nitrogen, carbohydrate, RNA and DNA. Physical tests, such as: cellulose acetate electrophoresis, disc gel electrophoresis, ultracentrifugation, isoelectricfocusing and gel filtration were also performed. Precipitation with ammonium sulfate provided PPD's with less impurities than those precipitated with trichloroacetic acid although there was little difference in the allergenic activity of the products precipitated by either method. Several lots of PPD-bovis were fractionated by means of ultrafiltration and the fractions analyzed by many of the above chemical and physical tests as well as tests for amino acid composition and allergenic activity. The greatest amount of protein was found in the fractions with an apparent molecular weight in excess of 300,000. The carbohydrate was usually distributed in proportion to the amount of protein in each fraction while the nucleic acids were predominately found in the higher molecular weight fractions. The amino acid analysis and allergenic activity of the various molecular weight fractions provided data that would support the theory that the various fractions are polymers of a small molecular weight sub-unit. The number of amino acid residues were calculated and the minimum molecular weight of the tubercular protein estimated to be 8700. The estimated molecular weights of the fractions and the amino acid data concur with those previously reported for proteins from other mycobacteria."}
{"id": "PMID:1097282", "title": "Adipose tissue cellularity in relation to metabolism in juvenile onset diabetes mellitus.", "content": "Adipose tissue cellularity was determined by a microscopic method in 18 men suffering from insulin dependent, juvenile diabetes mellitus for several years. These results were set in relation to the degree of clinical control estimated from the average urinary glucose output or fasting blood glucose during the year preceding the investigation. Furthermore, the results were compared with controls of comparable age and of the same sex. Both young and middleaged diabetic men had smaller fat cells than non-diabetic men. Fat cell size was correlated to the degree of clinical control, small fat cells indicating a poor control. It was suggested that fat cell size might be included as a measure of the long-term metabolic control in patients with insulin dependent diabetes mellitus.", "contents": "Adipose tissue cellularity in relation to metabolism in juvenile onset diabetes mellitus. Adipose tissue cellularity was determined by a microscopic method in 18 men suffering from insulin dependent, juvenile diabetes mellitus for several years. These results were set in relation to the degree of clinical control estimated from the average urinary glucose output or fasting blood glucose during the year preceding the investigation. Furthermore, the results were compared with controls of comparable age and of the same sex. Both young and middleaged diabetic men had smaller fat cells than non-diabetic men. Fat cell size was correlated to the degree of clinical control, small fat cells indicating a poor control. It was suggested that fat cell size might be included as a measure of the long-term metabolic control in patients with insulin dependent diabetes mellitus."}
{"id": "PMID:1097286", "title": "In vitro cellular responses to autologous tumor extract detected by inhibition of macrophage migration.", "content": "Indirect macrophage migration technique was employed to study in vitro cellular immune response to autologous tumor extract in man. Cellular hypersensitivity to autologous tumor extract was detected in 21 (26%) of 80 patients with various carcinomas preoperatively by means of the in vitro production of macrophage migration inhibitory factor (MIF). The relative frequency of MIF production in these patients varied inversely with progress of the diseases. The follow-up studies reveal postoperative decline of the cellular hypersensitivity after curative operation. These findings indicate that this assay can be applied for detecting cellular hypersensitivity to autologous tumor extracts in clinical situation.", "contents": "In vitro cellular responses to autologous tumor extract detected by inhibition of macrophage migration. Indirect macrophage migration technique was employed to study in vitro cellular immune response to autologous tumor extract in man. Cellular hypersensitivity to autologous tumor extract was detected in 21 (26%) of 80 patients with various carcinomas preoperatively by means of the in vitro production of macrophage migration inhibitory factor (MIF). The relative frequency of MIF production in these patients varied inversely with progress of the diseases. The follow-up studies reveal postoperative decline of the cellular hypersensitivity after curative operation. These findings indicate that this assay can be applied for detecting cellular hypersensitivity to autologous tumor extracts in clinical situation."}
{"id": "PMID:1097288", "title": "Pancreatic cholera. Sudies on tumoral secretions and pathophysiology of diarrhea.", "content": "Tumoral secretions and pathophysiology of diarrhea were studied in 1 patient with pancreatic cholera. High concentrations of vasoactive intestinal peptide were found in both systemic blood and tumoral extracts, together with increased plasma levels of calcitonin and protaglandins E and Falpha. Gastric inhibitory peptide and gastrointestinal and pancreatic hormones were absent from the tumor, except for small amounts of glucagon, and their blood levels were normal. Decreased basal but normal pentagastrin-stimulated gastric acid secretion, normal basal and secretin-stimulated pancreatic secretion, increased volume of gallbladder bile with high bicarbonate, and low bile salt concentrations were observed, but the electrolyte content and flow rate of fluid passing the duodenojejunal junction were within normal limits. Small intestine was found to be the origin of the water and electrolyte fasting losses. Jejunum was the site of bicarbonate secretion. Jejunal glucose and leucine-stimulated water and sodium transports were also strikingly decreased, whereas the absorption rates of the sugar and amino acid were normal. Colon reabsorbed high amounts of water and sodium but increased potassium losses. Biological effects of vasoactive intestinal peptide may explain most of the patient's upper digestive secretion abnormalities and small intestinal function impairments, whereas secondary aldosteronism might explain the modified colonic function.", "contents": "Pancreatic cholera. Sudies on tumoral secretions and pathophysiology of diarrhea. Tumoral secretions and pathophysiology of diarrhea were studied in 1 patient with pancreatic cholera. High concentrations of vasoactive intestinal peptide were found in both systemic blood and tumoral extracts, together with increased plasma levels of calcitonin and protaglandins E and Falpha. Gastric inhibitory peptide and gastrointestinal and pancreatic hormones were absent from the tumor, except for small amounts of glucagon, and their blood levels were normal. Decreased basal but normal pentagastrin-stimulated gastric acid secretion, normal basal and secretin-stimulated pancreatic secretion, increased volume of gallbladder bile with high bicarbonate, and low bile salt concentrations were observed, but the electrolyte content and flow rate of fluid passing the duodenojejunal junction were within normal limits. Small intestine was found to be the origin of the water and electrolyte fasting losses. Jejunum was the site of bicarbonate secretion. Jejunal glucose and leucine-stimulated water and sodium transports were also strikingly decreased, whereas the absorption rates of the sugar and amino acid were normal. Colon reabsorbed high amounts of water and sodium but increased potassium losses. Biological effects of vasoactive intestinal peptide may explain most of the patient's upper digestive secretion abnormalities and small intestinal function impairments, whereas secondary aldosteronism might explain the modified colonic function."}
{"id": "PMID:1097289", "title": "Synthesis and secretion of protein and pepsinogen by rabbit gastric mucosa in organ culture.", "content": "When maintained in organ culture, rabbit gastric mucosal biopsies incorporated [14tc]leucine into tissue protein and secreted labeled protein into culture medium steadily for 24 hr. Incorporation of radioactivity was abolished by cycloheximide. When examined by sodium dodecyl sulfate gel electrophoresis, dextran gel filtration, and ion exchange chromatography, 65 to 90% of macromolecular radioactivity secreted into culture medium migrated coincidentally with enzymatically assayed pepsinogen. Pepsin activity in cultured biopsies did not decrease during 24 hr of organ culture. Nevertheless, pepsin activity increased linearly in culture medium during this period. Acetylcholine markedly stimulated secretion of labeled protein and pepsinogen by cultured biopsies. In the presence of a subthreshold concentration (10(-10) M) of acetylcholine, pentagastrin, secretin, and the octapeptide of cholecystokinin, all stimulated protein secretion. Over-all incorporation of [14C]leucine into protein by cultured biopsies was stimulated by 10(-9) M pentagastrin. These results directly demonstrate: (1) synthesis and secretion of protein and pepsinogen by isolated gastric mucosa, (2) stimulation of gastric secretion of protein by acetylcholine and polypeptide hormones, and (3) stimulation of gastric synthesis of protein by pentagastrin.", "contents": "Synthesis and secretion of protein and pepsinogen by rabbit gastric mucosa in organ culture. When maintained in organ culture, rabbit gastric mucosal biopsies incorporated [14tc]leucine into tissue protein and secreted labeled protein into culture medium steadily for 24 hr. Incorporation of radioactivity was abolished by cycloheximide. When examined by sodium dodecyl sulfate gel electrophoresis, dextran gel filtration, and ion exchange chromatography, 65 to 90% of macromolecular radioactivity secreted into culture medium migrated coincidentally with enzymatically assayed pepsinogen. Pepsin activity in cultured biopsies did not decrease during 24 hr of organ culture. Nevertheless, pepsin activity increased linearly in culture medium during this period. Acetylcholine markedly stimulated secretion of labeled protein and pepsinogen by cultured biopsies. In the presence of a subthreshold concentration (10(-10) M) of acetylcholine, pentagastrin, secretin, and the octapeptide of cholecystokinin, all stimulated protein secretion. Over-all incorporation of [14C]leucine into protein by cultured biopsies was stimulated by 10(-9) M pentagastrin. These results directly demonstrate: (1) synthesis and secretion of protein and pepsinogen by isolated gastric mucosa, (2) stimulation of gastric secretion of protein by acetylcholine and polypeptide hormones, and (3) stimulation of gastric synthesis of protein by pentagastrin."}
{"id": "PMID:1097294", "title": "Metabolism of lithocholate in healthy man. II. Enterohepatic circulation.", "content": "Studies were carried out in healthy subjects to characterize the enterohepatic circulation of lithocholate and its metabolites. When mixed with bile and infused into the jejunum, radiolabeled lithocholylglycine was absorbed more rapidly and more efficiently than sulfolithocholylglycine, based on recovery from bile. When these metabolites were administered at 1800 hr in a liquid test meal containing radiolabeled taurocholate as an absorbable marker, 60% of lithocholylglycine was conserved, based on recovery of radioactivity in fasting bile the following morning, but only 20% of sulfolithocholylglycine was conserved. Iotope dilution studies in 4 subjects showed that daily input of lithocholate into the bile acid pool averaged 100 mg per day, about one-third to one-half of the chenodeoxycholic acid synthesis, but the t 1/2 was extremely short (0.74 day). The small lithocholate pool (about 100 mg) could be explained by rapid fecal excretion caused by sulfation which decreases passive absorption in the jejunum and active absorption in the ileum. Experiments with [35S]sulfo- [3H]lithocholylglycine indicated little desulfation during enterohepatic cycling but rapid desulfation in the distal intestine, with absorption of 35S (presumably as sulfate) followed by urinary excretion. A decreasing 35S:3H ratio in bile indicated that some steroid moiety was conserved to be resulfated. These studies indicate that considerable lithocholate is absorbed from the distal intestine in healthy subjects but efficient sulfation results in rapid fecal excretion, so that the total lithocholate pool remains small. A multicompartment model, previously used to describe the metabolism of the steroid and amino acid moieties of the major conjugated biliary bile acids, was extended to encompass lithocholyl conjugates and their sulfates.", "contents": "Metabolism of lithocholate in healthy man. II. Enterohepatic circulation. Studies were carried out in healthy subjects to characterize the enterohepatic circulation of lithocholate and its metabolites. When mixed with bile and infused into the jejunum, radiolabeled lithocholylglycine was absorbed more rapidly and more efficiently than sulfolithocholylglycine, based on recovery from bile. When these metabolites were administered at 1800 hr in a liquid test meal containing radiolabeled taurocholate as an absorbable marker, 60% of lithocholylglycine was conserved, based on recovery of radioactivity in fasting bile the following morning, but only 20% of sulfolithocholylglycine was conserved. Iotope dilution studies in 4 subjects showed that daily input of lithocholate into the bile acid pool averaged 100 mg per day, about one-third to one-half of the chenodeoxycholic acid synthesis, but the t 1/2 was extremely short (0.74 day). The small lithocholate pool (about 100 mg) could be explained by rapid fecal excretion caused by sulfation which decreases passive absorption in the jejunum and active absorption in the ileum. Experiments with [35S]sulfo- [3H]lithocholylglycine indicated little desulfation during enterohepatic cycling but rapid desulfation in the distal intestine, with absorption of 35S (presumably as sulfate) followed by urinary excretion. A decreasing 35S:3H ratio in bile indicated that some steroid moiety was conserved to be resulfated. These studies indicate that considerable lithocholate is absorbed from the distal intestine in healthy subjects but efficient sulfation results in rapid fecal excretion, so that the total lithocholate pool remains small. A multicompartment model, previously used to describe the metabolism of the steroid and amino acid moieties of the major conjugated biliary bile acids, was extended to encompass lithocholyl conjugates and their sulfates."}
{"id": "PMID:1097295", "title": "A controlled trial of azathioprine in the management of chronic ulcerative colitis.", "content": "To determine the efficacy of azathioprine in the treatment of ulcerative colitis, a 6-month double blind trial was carried out. Thirty patients with chronic ulcerative colitis who required the equivalent of at least 10 mg of prednisone per day over the 3 months prior to entering the study were randomized into placebo and azathioprine (1.5 mg per kg) treatment groups. Reduction of steriods was a major objective of the trial. Age and sex distribution, number of bowel movements, sense of well being, steroid dosage, and findings on proctoscopy, rectal biopsy, and colon X-ray initially were similar in the two groups. No side effects were associated with azathioprine. Although steroid dose was lower (p less than 0.05) in the azathioprine group at the termination of the study, no difference between the two groups could be detected in the number of bowel movements, sense of well being, and findings on proctoscopy during the first 3 weeks compared with the last 3 and during the first 3 months compared with the last 3. Although azathioprine does not confer dramatic benefit upon patients with chronic ulcerative colitis who require steroids, it does permit reduction of steroid dosage without apparent worsening of the disease. Its major value in ulcerative colitis may be in facilitating significant decreases or complete discontinuance of steroids.", "contents": "A controlled trial of azathioprine in the management of chronic ulcerative colitis. To determine the efficacy of azathioprine in the treatment of ulcerative colitis, a 6-month double blind trial was carried out. Thirty patients with chronic ulcerative colitis who required the equivalent of at least 10 mg of prednisone per day over the 3 months prior to entering the study were randomized into placebo and azathioprine (1.5 mg per kg) treatment groups. Reduction of steriods was a major objective of the trial. Age and sex distribution, number of bowel movements, sense of well being, steroid dosage, and findings on proctoscopy, rectal biopsy, and colon X-ray initially were similar in the two groups. No side effects were associated with azathioprine. Although steroid dose was lower (p less than 0.05) in the azathioprine group at the termination of the study, no difference between the two groups could be detected in the number of bowel movements, sense of well being, and findings on proctoscopy during the first 3 weeks compared with the last 3 and during the first 3 months compared with the last 3. Although azathioprine does not confer dramatic benefit upon patients with chronic ulcerative colitis who require steroids, it does permit reduction of steroid dosage without apparent worsening of the disease. Its major value in ulcerative colitis may be in facilitating significant decreases or complete discontinuance of steroids."}
{"id": "PMID:1097296", "title": "Complications of endoscopic retrograde cholangiopancreatography. Analysis of 300 consecutive cases.", "content": "A retrospective analysis of complications arising from 300 consecutive attempts at endoscopic retrograde cholangiopancreatography (ERCP) in 278 patients was undetaken to determine the rate and severity of complications. An over-all complication rate of 5% (15 cases) was documented. Complications were categorized in terms of those arising from endoscopy itself or from the administration of pharmacological agents (7 cases), and those observed after the injection of radioopaque contrast into the biliary tree or pancreas (8 cases). Complications which might be considered coincidental to a patient's underlying illness were not excluded. Complications were significantly more frequent after injection of diseased duct systems. Brief, self-limited pancreatitis after retrograde pancreatography occurred in 5 of 90 patients with pancreatic disease. No cases of pancreatitis were observed after retrograde pancreatography in 102 patients without pancreatic disease X2 = 5.82, P less than 0.025). Sepsis occurred after retrograde cholangiography in 3 of 56 patients with extrahepatic biliary obstruction. In the absence of extrahepatic obstruction, cholangiography was performed without complication in 85 cases (X2 = 3.62, P less than 0.1), although 25 of these had intense cholestasis due to hepatic parenchymal disease. This analysis provides the basis for modifications of ERCP technique and management that may reduce the future incidence of complications. This study suggests that the incidence and severity of complications that arise from ERCP compare favorably with procedures of equivalent diagnostic yield.", "contents": "Complications of endoscopic retrograde cholangiopancreatography. Analysis of 300 consecutive cases. A retrospective analysis of complications arising from 300 consecutive attempts at endoscopic retrograde cholangiopancreatography (ERCP) in 278 patients was undetaken to determine the rate and severity of complications. An over-all complication rate of 5% (15 cases) was documented. Complications were categorized in terms of those arising from endoscopy itself or from the administration of pharmacological agents (7 cases), and those observed after the injection of radioopaque contrast into the biliary tree or pancreas (8 cases). Complications which might be considered coincidental to a patient's underlying illness were not excluded. Complications were significantly more frequent after injection of diseased duct systems. Brief, self-limited pancreatitis after retrograde pancreatography occurred in 5 of 90 patients with pancreatic disease. No cases of pancreatitis were observed after retrograde pancreatography in 102 patients without pancreatic disease X2 = 5.82, P less than 0.025). Sepsis occurred after retrograde cholangiography in 3 of 56 patients with extrahepatic biliary obstruction. In the absence of extrahepatic obstruction, cholangiography was performed without complication in 85 cases (X2 = 3.62, P less than 0.1), although 25 of these had intense cholestasis due to hepatic parenchymal disease. This analysis provides the basis for modifications of ERCP technique and management that may reduce the future incidence of complications. This study suggests that the incidence and severity of complications that arise from ERCP compare favorably with procedures of equivalent diagnostic yield."}
{"id": "PMID:1097297", "title": "Bacterial studies of Clindamycin-associated colitis. A preliminary report.", "content": "The cause of pseudomembraneous colitis is not known but has been attributed to an alteration in the microbial flora of the colon. To test this hypothesis, a blind, prospective study was undertaken in which fecal samples were cultured quantitatively for aerobic and anaerobic organisms. The patients from which these samples were taken were all receiving clindamycin and had diarrhea secondary to the use of the drug. We were able to show definite differences in the colonic microflora which correlated with the presence of pseudomembranes on biopsy. Those patients who had diarrhea and pseudomembranous colitis showed a striking decrease, both quantitatively and qualitatively, in the number of anaerobes present. Those patients who had diarrhea but no pseudomembranes had large numbers of anaerobes which qualitatively approximated normal fecal cultures but quantitatively were fewer in number. A third group of patients, which had resolving pseudomembranous colitis, and were no longer symptomatic, had large numbers of anaerobic organisms which approximated those found in normal fecal cultures. There were no differences among the three groups with regard to facultative anaerobic microflora. Thus, the presence of pseudomembranous colitis correlated directly with a striking quantitative and qualitative decrease in the anaerobic microflora of the colon. The symptom of diarrhea alone appeared to have no meaning with regard to changes in the bacterial flora. Resolution of pseudomembranous colitis was associated with a return of the anaerobic microflora.", "contents": "Bacterial studies of Clindamycin-associated colitis. A preliminary report. The cause of pseudomembraneous colitis is not known but has been attributed to an alteration in the microbial flora of the colon. To test this hypothesis, a blind, prospective study was undertaken in which fecal samples were cultured quantitatively for aerobic and anaerobic organisms. The patients from which these samples were taken were all receiving clindamycin and had diarrhea secondary to the use of the drug. We were able to show definite differences in the colonic microflora which correlated with the presence of pseudomembranes on biopsy. Those patients who had diarrhea and pseudomembranous colitis showed a striking decrease, both quantitatively and qualitatively, in the number of anaerobes present. Those patients who had diarrhea but no pseudomembranes had large numbers of anaerobes which qualitatively approximated normal fecal cultures but quantitatively were fewer in number. A third group of patients, which had resolving pseudomembranous colitis, and were no longer symptomatic, had large numbers of anaerobic organisms which approximated those found in normal fecal cultures. There were no differences among the three groups with regard to facultative anaerobic microflora. Thus, the presence of pseudomembranous colitis correlated directly with a striking quantitative and qualitative decrease in the anaerobic microflora of the colon. The symptom of diarrhea alone appeared to have no meaning with regard to changes in the bacterial flora. Resolution of pseudomembranous colitis was associated with a return of the anaerobic microflora."}
{"id": "PMID:1097298", "title": "Inhibition of gastric acid secretion in man by the transcranial application of lwo intensity pulsed current.", "content": "This study was conducted to determine the effectiveness of transcranial electrotherapy (TCE) in reducing gastric secretion in man. TCE has been proposed as a therapeutic modality which induces a relaxed psychological state by the application of low intensity diffuse electrical current and has been purported by Soviet investigators to be beneficial in the treatment of peptic ulcers. Secretion rates were monitored in adult male volunteers by a method of intragastric titration utilizing a pH-sensitive telemetry capsule. In one study 5 subjects had their basal secretion rates monitored before, during, and after the application of TCE at graduated current intensities. A threshold of inhibition was observed for currents of 0.9 ma and greater. In a second study, 12 subjects had histamine-stimulated maximum acid output determined for control and during TCE application. Gastric acid secretion was reduced by an average of 30% when 1-ma TCE was applied, with individual reductions ranging from 5.7% to 53.2%. Since the application of relatively high TCE currents may produce discomfort in some subjects, a third study was conducted to determine whether the inhibition might not merely be the result of nonspecific noxious stimuli. For this study the electrical connections to the TCE electrodes were altered so that the same uncomfortable sensation was produced on the forehead, but no current was actually applied transcranially. This \"placebo\" TCE produced no reduction in the maximal acid output of 6 volunteers, but when the currents were applied transcranially, the gastric acid secretion was reduced by an average of 27% below control values.", "contents": "Inhibition of gastric acid secretion in man by the transcranial application of lwo intensity pulsed current. This study was conducted to determine the effectiveness of transcranial electrotherapy (TCE) in reducing gastric secretion in man. TCE has been proposed as a therapeutic modality which induces a relaxed psychological state by the application of low intensity diffuse electrical current and has been purported by Soviet investigators to be beneficial in the treatment of peptic ulcers. Secretion rates were monitored in adult male volunteers by a method of intragastric titration utilizing a pH-sensitive telemetry capsule. In one study 5 subjects had their basal secretion rates monitored before, during, and after the application of TCE at graduated current intensities. A threshold of inhibition was observed for currents of 0.9 ma and greater. In a second study, 12 subjects had histamine-stimulated maximum acid output determined for control and during TCE application. Gastric acid secretion was reduced by an average of 30% when 1-ma TCE was applied, with individual reductions ranging from 5.7% to 53.2%. Since the application of relatively high TCE currents may produce discomfort in some subjects, a third study was conducted to determine whether the inhibition might not merely be the result of nonspecific noxious stimuli. For this study the electrical connections to the TCE electrodes were altered so that the same uncomfortable sensation was produced on the forehead, but no current was actually applied transcranially. This \"placebo\" TCE produced no reduction in the maximal acid output of 6 volunteers, but when the currents were applied transcranially, the gastric acid secretion was reduced by an average of 27% below control values."}
{"id": "PMID:1097299", "title": "Effect of prolonged administration of homologous and heterologous intrinsic factor antibodies on the parietal and peptic cell masses and the secretory function of the rat gastric mucosa.", "content": "In order to determine the possible effects of the circulating intrinsic factor antibodies (IFA) on gastric morphology and secretory function, four groups of 12 rats each received intravenous injection daily for 8 to 12 weeks of immunoglobulin G (IgG) fractions separated on DEAE-cellulose columns from various sources: (1) sera of patients with pernicious anemia, containing both IFA and parietal cell antibodies (PCA), (2) sera from patients with atrophic gastritis, containing parietal cell antibody only, and (3) and (4) sera of rabbits immunized with semipurified human or rat intrinsic factor (IF). In addition three control groups of 12 rats each received intravenous injections daily for 8 to 12 weeks of either (5) saline or (6) and (7) IgG processed from human or rabbit normal sera. Still another group of 12 rats (8) did not receive any injections whatsoever for the same duration of time. One-third of the rats were intubated biweekly after histamine stimulation and the hourly outputs of CHl pepsin, and IF were determined. At conclusion of the experiments, rats were killed, the mucosal surface and thickness of the mucosa were measured, and parietal cell and peptic cell masses were counted. The control groups showed either progressive growth of the cellular mass in gastric mucosa and increase of the HCl, pepsin, and IF outputs, or no significant changes. In contrast, rats injected with IgG containing IFA to human or rat IF showed a statistically significant thinning of the gastric mucosa, reduction of peptic cells, which are known to secret IF in this species, and corresponding decreases in the ouputs of pepsin and IF. These became reduced by about 50% from initial values, and by 62 or 75%, respectively, when compared to rats injected with normal human or rabbit IgG's. In rats injected with IgG's from pernicious anemia sera, which contained both IFA and PCA, the outputs of IF, pepsin, and HCl decreased signigicantly, as well as the peptic and parietal cell masses. The rats injected with PCA only demonstrated thinning of the gastric mucosa, reduction of parietal cell mass, and a significant decrease of the HCl output. These findings imply an active role of the circulating gastric.", "contents": "Effect of prolonged administration of homologous and heterologous intrinsic factor antibodies on the parietal and peptic cell masses and the secretory function of the rat gastric mucosa. In order to determine the possible effects of the circulating intrinsic factor antibodies (IFA) on gastric morphology and secretory function, four groups of 12 rats each received intravenous injection daily for 8 to 12 weeks of immunoglobulin G (IgG) fractions separated on DEAE-cellulose columns from various sources: (1) sera of patients with pernicious anemia, containing both IFA and parietal cell antibodies (PCA), (2) sera from patients with atrophic gastritis, containing parietal cell antibody only, and (3) and (4) sera of rabbits immunized with semipurified human or rat intrinsic factor (IF). In addition three control groups of 12 rats each received intravenous injections daily for 8 to 12 weeks of either (5) saline or (6) and (7) IgG processed from human or rabbit normal sera. Still another group of 12 rats (8) did not receive any injections whatsoever for the same duration of time. One-third of the rats were intubated biweekly after histamine stimulation and the hourly outputs of CHl pepsin, and IF were determined. At conclusion of the experiments, rats were killed, the mucosal surface and thickness of the mucosa were measured, and parietal cell and peptic cell masses were counted. The control groups showed either progressive growth of the cellular mass in gastric mucosa and increase of the HCl, pepsin, and IF outputs, or no significant changes. In contrast, rats injected with IgG containing IFA to human or rat IF showed a statistically significant thinning of the gastric mucosa, reduction of peptic cells, which are known to secret IF in this species, and corresponding decreases in the ouputs of pepsin and IF. These became reduced by about 50% from initial values, and by 62 or 75%, respectively, when compared to rats injected with normal human or rabbit IgG's. In rats injected with IgG's from pernicious anemia sera, which contained both IFA and PCA, the outputs of IF, pepsin, and HCl decreased signigicantly, as well as the peptic and parietal cell masses. The rats injected with PCA only demonstrated thinning of the gastric mucosa, reduction of parietal cell mass, and a significant decrease of the HCl output. These findings imply an active role of the circulating gastric."}
{"id": "PMID:1097300", "title": "[The risk of yeast infection, of the newborn during and after delivery (author's transl)].", "content": "The importance of yeast infections in obstetrics was investigated in 200 pregnant women and their newborns. In the maternity patients the vagina was tested shortly before the delivery and the mouth of the neonates was tested 1 day prior to the discharge from the hospital. In 22% of the pregnant women and in 8,4% of the newborns, yeast (predominantly candida albicans) was found in the culture. Infants of mothers with positive cultures for yeast showed yeast more frequently (13,2%) themselves as infants of mothers with negative cultures for yeast (7,2%). Only 1 infant had clinical trush. Only 1/7 of the women who had positive cultures for yeast at delivery also had positive cultures for yeast 5--8 weeks following delivery although they were not treated. Second to the maternal vagina, the mouth of the mothers and the nursing personnel are the most important sources for yeast infections of the newborn infant. The demand to make the birth canal free of yeast at the end of the pregnancy does not appear to be justified for all obstetric units according to the results of our investigations. The incidence of a clinical yeast infection in the mature and healthy newborn infant was between 0,5 and 0,9% during the past 2 1/2 years at the Obstetric Department of the University of Erlangen, Germany. Prophylactic medication against yeast is important for all high risk neonates such as premature infants, infants with asphyxia and infants under antibiotic treatment.", "contents": "[The risk of yeast infection, of the newborn during and after delivery (author's transl)]. The importance of yeast infections in obstetrics was investigated in 200 pregnant women and their newborns. In the maternity patients the vagina was tested shortly before the delivery and the mouth of the neonates was tested 1 day prior to the discharge from the hospital. In 22% of the pregnant women and in 8,4% of the newborns, yeast (predominantly candida albicans) was found in the culture. Infants of mothers with positive cultures for yeast showed yeast more frequently (13,2%) themselves as infants of mothers with negative cultures for yeast (7,2%). Only 1 infant had clinical trush. Only 1/7 of the women who had positive cultures for yeast at delivery also had positive cultures for yeast 5--8 weeks following delivery although they were not treated. Second to the maternal vagina, the mouth of the mothers and the nursing personnel are the most important sources for yeast infections of the newborn infant. The demand to make the birth canal free of yeast at the end of the pregnancy does not appear to be justified for all obstetric units according to the results of our investigations. The incidence of a clinical yeast infection in the mature and healthy newborn infant was between 0,5 and 0,9% during the past 2 1/2 years at the Obstetric Department of the University of Erlangen, Germany. Prophylactic medication against yeast is important for all high risk neonates such as premature infants, infants with asphyxia and infants under antibiotic treatment."}
{"id": "PMID:1097302", "title": "Elevated mutability of polA derivatives of Escherichia coli B/r at sublethal doses of ultraviolet light: evidence for an inducible error-prone repair system (\"SOS repair\") and its anomalous expression in these strains.", "content": "The SOS repair hypothesis proposes that UV-induced mutations in E. coli are caused by an inducible error-prone repair system (SOS repair) which is normally induced coordinately with such other recApluslexplus-dependent functions as filamentous growth, prophage induction and W-reactivation-2 in response to UV or other inhibitors of DNA synthesis. Since polA-l strains induce these functions at unusually low doses of UV, the SOS hypothesis predicts elevated UV mutability for such strains at these low doses (50 ergs per mm2 and below) Strain WP6, a polA-1 derivative of B/r, exhibits the predicted high UV mutability in this dose range, producing ten times as many Trp+ mutations as its polA+ counterpart, strain WP1, at a dose of 12.5 ergs per mm-2. The UV sensitivity of a lex polA double mutant, in which the lex and polA mutations fail to exert their individual effects on UV sensitivity additively, also confirms a prediction generated by the SOS hypothesis.--The uvrA polA-1 strain WP67 also shows elevated UV mutability at very low UV doses (5 ergs per mm-2 and below), producing about ten times as many Trp+ mutations at a dose of 0.6 ergs per mm-2 as its uvrA polA+ parent strain WP2s. It is proposed that the double mutant induces the SOS repair system (and probably other \"reclex\" inducible functions) at unusually low doses of UV as a consequence of its relatively inefficient repair of DNA damage when plated on broth-supplemented media.", "contents": "Elevated mutability of polA derivatives of Escherichia coli B/r at sublethal doses of ultraviolet light: evidence for an inducible error-prone repair system (\"SOS repair\") and its anomalous expression in these strains. The SOS repair hypothesis proposes that UV-induced mutations in E. coli are caused by an inducible error-prone repair system (SOS repair) which is normally induced coordinately with such other recApluslexplus-dependent functions as filamentous growth, prophage induction and W-reactivation-2 in response to UV or other inhibitors of DNA synthesis. Since polA-l strains induce these functions at unusually low doses of UV, the SOS hypothesis predicts elevated UV mutability for such strains at these low doses (50 ergs per mm2 and below) Strain WP6, a polA-1 derivative of B/r, exhibits the predicted high UV mutability in this dose range, producing ten times as many Trp+ mutations as its polA+ counterpart, strain WP1, at a dose of 12.5 ergs per mm-2. The UV sensitivity of a lex polA double mutant, in which the lex and polA mutations fail to exert their individual effects on UV sensitivity additively, also confirms a prediction generated by the SOS hypothesis.--The uvrA polA-1 strain WP67 also shows elevated UV mutability at very low UV doses (5 ergs per mm-2 and below), producing about ten times as many Trp+ mutations at a dose of 0.6 ergs per mm-2 as its uvrA polA+ parent strain WP2s. It is proposed that the double mutant induces the SOS repair system (and probably other \"reclex\" inducible functions) at unusually low doses of UV as a consequence of its relatively inefficient repair of DNA damage when plated on broth-supplemented media."}
{"id": "PMID:1097311", "title": "[Mechanisms of release of leucocytic pyrogen].", "content": "Mechanisms of the production and release of leucocytic pyrogen (LP) from peritoneal polymorphonuclear leucocytes (PMN) from rabbits were studied in vitro. The following results were obtained. 1) The fever curves of the microsomal and the lysosomal fractions had a later onset and were longer lasting than those from the extracellular fluid. The fever curves of the supernatant of 105,000 g showed the typical response of endogenous pyrogen characterized by a rapid onset and short lasting fever, as shown by the extracellular fluid. 2) The pyrogenicity of LP was the most potent at 60 min while the 105,000 g supernatant was most potent at 30 min after PMN incubation at 37 degrees C. 3) The extracellular protein attained the maximum level at 30 min after incubation of PMN, while the protein content in the supernatant of 105,000 g of PMN decreased gradually to the constant level at 30 min. 4) It was observed that the lysosomal degradation was stimulated with bacterial pyrogen (LPS) at 37 degrees C but LPS did not directly affect the lysosomal fraction of PMN.", "contents": "[Mechanisms of release of leucocytic pyrogen]. Mechanisms of the production and release of leucocytic pyrogen (LP) from peritoneal polymorphonuclear leucocytes (PMN) from rabbits were studied in vitro. The following results were obtained. 1) The fever curves of the microsomal and the lysosomal fractions had a later onset and were longer lasting than those from the extracellular fluid. The fever curves of the supernatant of 105,000 g showed the typical response of endogenous pyrogen characterized by a rapid onset and short lasting fever, as shown by the extracellular fluid. 2) The pyrogenicity of LP was the most potent at 60 min while the 105,000 g supernatant was most potent at 30 min after PMN incubation at 37 degrees C. 3) The extracellular protein attained the maximum level at 30 min after incubation of PMN, while the protein content in the supernatant of 105,000 g of PMN decreased gradually to the constant level at 30 min. 4) It was observed that the lysosomal degradation was stimulated with bacterial pyrogen (LPS) at 37 degrees C but LPS did not directly affect the lysosomal fraction of PMN."}
{"id": "PMID:1097315", "title": "Glucagon biosynthesis in human pancreatic islets: preliminary evidence for a biosynthetic intermediate.", "content": "Viable pancreatic islets were isolated from the pancreas of humans using modifications of the collagenase digestion and Ficoll gradient techniques. Gel filtration of tissue extracts following islet incubation in the presence of 3H- tryptophan indicated that radioactivity becomes incorporated into at least two islet proteins. The larger of the two (LGI) has the approximate molecular size of proinsuiln and the smaller coelutes with glucagon as determined by column standardization. Radioimmunoassay of the gel filtration eluate for glucagon revealed that both molecules have glucagon immunoreactivity. Gel filtration in the presence of 8M urea did not alter the elution pattern of the LGI molecule. Polyacrylamide gel electrophoresis was performed on these glucagon immunoreactive molecules. The 3H radioactivity and the glucagon immunoreactivity of the smaller molecule were found to co-migrate electrophoretically with crystalline porcine glucagon and monodesamidoglucagon. With electrophoresis at high pH the electrophoretic mobility of the LGI molecule proved to be lower than that of glucagon. Partial tryptic degradation of the human LGI molecule yields 3H-tryptophan labeled products having charge and immunologic characteristics indistinguishable from porcine glucagon and monodesamidoglucagon. Although further investigation is indicatend may thus serve as a precursor or an intermediate in human glucagon biosynthesis.", "contents": "Glucagon biosynthesis in human pancreatic islets: preliminary evidence for a biosynthetic intermediate. Viable pancreatic islets were isolated from the pancreas of humans using modifications of the collagenase digestion and Ficoll gradient techniques. Gel filtration of tissue extracts following islet incubation in the presence of 3H- tryptophan indicated that radioactivity becomes incorporated into at least two islet proteins. The larger of the two (LGI) has the approximate molecular size of proinsuiln and the smaller coelutes with glucagon as determined by column standardization. Radioimmunoassay of the gel filtration eluate for glucagon revealed that both molecules have glucagon immunoreactivity. Gel filtration in the presence of 8M urea did not alter the elution pattern of the LGI molecule. Polyacrylamide gel electrophoresis was performed on these glucagon immunoreactive molecules. The 3H radioactivity and the glucagon immunoreactivity of the smaller molecule were found to co-migrate electrophoretically with crystalline porcine glucagon and monodesamidoglucagon. With electrophoresis at high pH the electrophoretic mobility of the LGI molecule proved to be lower than that of glucagon. Partial tryptic degradation of the human LGI molecule yields 3H-tryptophan labeled products having charge and immunologic characteristics indistinguishable from porcine glucagon and monodesamidoglucagon. Although further investigation is indicatend may thus serve as a precursor or an intermediate in human glucagon biosynthesis."}
{"id": "PMID:1097317", "title": "Synthesis of a chemically reactive analog of the initiation codon: its reaction with ribosomes of Escherichia coli.", "content": "Nitrophenylated 5'-adenylic acid could be employed as primer in a polyribonucleotide nucleotidyltransferase (Micrococcus luteus) reaction to yield 5'-nitrophenylated pA-U-G. After reduction and subsequent bromoacetylation, an A-U-G analog was obtained, which could be used as an affinity label for the ribosomal A-U-G-binding site(s). After incubating the A-U-G affinity label with 70S ribosomes, 30S subunits programmed for initiation-factor-dependent fMet-tRNAMetf binding were obtained. Hence, the A-U-G analog had irreversibly reacted at the ribosomal decoding site. Initiation complexes which were formed with the labeled 30S subunits were puromycin-resistant. Furthermore, GTP hydrolysis, necessary for proper accommodation of initiator tRNA at the ribosomal donorsite, did not function in these complexes. These data indicate that immobilization of A-U-G at the decoding site of the ribosome allows factor-dependent initiator tRNA binding, but impairs accommodation at the donor site. The ribosomal protein(s) to which A-U-G was covalently bound at the decoding site were identified by polyacrylamide gel electrophoresis in the presence of urea or sarkosyl. The predominant affinity-labeled protein was found to be protein S18. Variation of the incubation conditions of the affinity-labeling reaction leads to attachment of A-U-G label to another ribosomal protein, S4, the ram gene product.", "contents": "Synthesis of a chemically reactive analog of the initiation codon: its reaction with ribosomes of Escherichia coli. Nitrophenylated 5'-adenylic acid could be employed as primer in a polyribonucleotide nucleotidyltransferase (Micrococcus luteus) reaction to yield 5'-nitrophenylated pA-U-G. After reduction and subsequent bromoacetylation, an A-U-G analog was obtained, which could be used as an affinity label for the ribosomal A-U-G-binding site(s). After incubating the A-U-G affinity label with 70S ribosomes, 30S subunits programmed for initiation-factor-dependent fMet-tRNAMetf binding were obtained. Hence, the A-U-G analog had irreversibly reacted at the ribosomal decoding site. Initiation complexes which were formed with the labeled 30S subunits were puromycin-resistant. Furthermore, GTP hydrolysis, necessary for proper accommodation of initiator tRNA at the ribosomal donorsite, did not function in these complexes. These data indicate that immobilization of A-U-G at the decoding site of the ribosome allows factor-dependent initiator tRNA binding, but impairs accommodation at the donor site. The ribosomal protein(s) to which A-U-G was covalently bound at the decoding site were identified by polyacrylamide gel electrophoresis in the presence of urea or sarkosyl. The predominant affinity-labeled protein was found to be protein S18. Variation of the incubation conditions of the affinity-labeling reaction leads to attachment of A-U-G label to another ribosomal protein, S4, the ram gene product."}
{"id": "PMID:1097318", "title": "PSRO's and peer review: problems and opportunities.", "content": "Legislation passed in 1972 mandated the establisment of Professional Standards Review Organizationa to review the quality of medical services to beneficiaries of the Medicare, Medicaid, and the Maternal and Child Health programs. Physicians in all 203 designated PSRO areas must show a viable organization by January 1976, or a nonphysician group may be designated as a PSRO. The author discusses the provisions of the law, reactions of physicians and others, the law's alleged shortcomings, and the questions that arise as a result of its passage. He cites some preliminary studies in the psychiatric literature that report positive effects of reviews of patient care.", "contents": "PSRO's and peer review: problems and opportunities. Legislation passed in 1972 mandated the establisment of Professional Standards Review Organizationa to review the quality of medical services to beneficiaries of the Medicare, Medicaid, and the Maternal and Child Health programs. Physicians in all 203 designated PSRO areas must show a viable organization by January 1976, or a nonphysician group may be designated as a PSRO. The author discusses the provisions of the law, reactions of physicians and others, the law's alleged shortcomings, and the questions that arise as a result of its passage. He cites some preliminary studies in the psychiatric literature that report positive effects of reviews of patient care."}
{"id": "PMID:1097319", "title": "A psychiatric POMR for use by a multidisciplinary team.", "content": "A psychiatric problem-oriented medical record designed for use by multidisciplinary teams was introduced at Fairfield Hills Hospital in Newtown, Connecticut, in 1972. The need for revision in the hospital's medical record system had been pointed up by the development of the teams, which shared more equally in responsibility for patient care, and by problems in meeting Medicare standards. The authors describe the various forms that make up the POMR. Most of the forms are completed by the teams in regularly scheduled conferences.", "contents": "A psychiatric POMR for use by a multidisciplinary team. A psychiatric problem-oriented medical record designed for use by multidisciplinary teams was introduced at Fairfield Hills Hospital in Newtown, Connecticut, in 1972. The need for revision in the hospital's medical record system had been pointed up by the development of the teams, which shared more equally in responsibility for patient care, and by problems in meeting Medicare standards. The authors describe the various forms that make up the POMR. Most of the forms are completed by the teams in regularly scheduled conferences."}
{"id": "PMID:1097321", "title": "Structural and ultrastructural alterations, exfoliative cytology and enzyme cytochemistry and histochemistry, proliferation kinetics, immunological derangements and other causes, and clinical associations and sequallae.", "content": "Our main purpose in this review article is to present some of the vast amount of information on the subject of atrophic gastritis available at the present time. Various areas of research pertaining to this entity, including that from the author's laboratory, are reviewed in this article, encompassing histological and ultrastructural abnormalities, enzyme histochemistry, and exfoliative cytology and cytochemistry of chronic gastritis, with emphasis on the diagnostic significance of these laboratory methods. The altered cell proliferation kinetics in atrophic gastric lesions is also discussed, as well as its significance in the proliferation and reduction of glandular cells of the gastric mucosa. Furthermore, some of the more recent evidence of immunological mechanisms and other causes of chronic gastritis, such as chronic alcoholism and bile reflux, is reviewed, as well as the effects of partial gastrectomy on the gastric mucosa. The important associations and sequellae of atrophic gastritis, such as gastric ulcer, gastric polyps, and gastric cancer, are discussed in detail.", "contents": "Structural and ultrastructural alterations, exfoliative cytology and enzyme cytochemistry and histochemistry, proliferation kinetics, immunological derangements and other causes, and clinical associations and sequallae. Our main purpose in this review article is to present some of the vast amount of information on the subject of atrophic gastritis available at the present time. Various areas of research pertaining to this entity, including that from the author's laboratory, are reviewed in this article, encompassing histological and ultrastructural abnormalities, enzyme histochemistry, and exfoliative cytology and cytochemistry of chronic gastritis, with emphasis on the diagnostic significance of these laboratory methods. The altered cell proliferation kinetics in atrophic gastric lesions is also discussed, as well as its significance in the proliferation and reduction of glandular cells of the gastric mucosa. Furthermore, some of the more recent evidence of immunological mechanisms and other causes of chronic gastritis, such as chronic alcoholism and bile reflux, is reviewed, as well as the effects of partial gastrectomy on the gastric mucosa. The important associations and sequellae of atrophic gastritis, such as gastric ulcer, gastric polyps, and gastric cancer, are discussed in detail."}
{"id": "PMID:1097325", "title": "Education and training in electron microscopy.", "content": "There is currently a need for diagnostic electron microscopy in both autopsy and surgical pathology. As more information emerges from the research laboratories, applied electron microscopy will grow in depth, and all large medical centers will need the expertise provided by a diagnostic electron microscopy laboratory. Many physicians other than electron microscopists find it essential to have an understanding of the contributions and limitations of electron microscopy. Education and training programs therefore must encompass not only paramedical personnel who prepare the electron micrographs and pathologists who are thoroughly trained in interpret the electron micrographs, but also other physicians and scientists who utilize the information obtained therefrom. Medical students should obtain sufficient background in normal and abnormal ultrastructure to enable them to interpret the medical literature, and the means to obtain this background should be available within the medical school curriculum. Some medical students will also desire more thorough training obtained by elective courses in electron microscopy. Pathology residents should obtain sufficient expertise during their residency to enable them to utilize the information produced by the electron microscopy laboratory. Certain pathology residents and some pathologists in practice prefer fellowships for more specialized training in electron microscopy. Four representative training programs in electron microscopy from Veterans Administration hospitals have been selected for presentation. Each emphasizes a different approach and different objectives.", "contents": "Education and training in electron microscopy. There is currently a need for diagnostic electron microscopy in both autopsy and surgical pathology. As more information emerges from the research laboratories, applied electron microscopy will grow in depth, and all large medical centers will need the expertise provided by a diagnostic electron microscopy laboratory. Many physicians other than electron microscopists find it essential to have an understanding of the contributions and limitations of electron microscopy. Education and training programs therefore must encompass not only paramedical personnel who prepare the electron micrographs and pathologists who are thoroughly trained in interpret the electron micrographs, but also other physicians and scientists who utilize the information obtained therefrom. Medical students should obtain sufficient background in normal and abnormal ultrastructure to enable them to interpret the medical literature, and the means to obtain this background should be available within the medical school curriculum. Some medical students will also desire more thorough training obtained by elective courses in electron microscopy. Pathology residents should obtain sufficient expertise during their residency to enable them to utilize the information produced by the electron microscopy laboratory. Certain pathology residents and some pathologists in practice prefer fellowships for more specialized training in electron microscopy. Four representative training programs in electron microscopy from Veterans Administration hospitals have been selected for presentation. Each emphasizes a different approach and different objectives."}
{"id": "PMID:1097326", "title": "Porokeratosis [correction of Parakeratosis] Mibelli and skin carcinoma: a critical review.", "content": "The literature on porokeratosis [corrected] Mibelli (PM) is revised critically with respect to the development of skin carcinoma. The incidence of epidermal malignancies in PM lesions is 7% (17 of 250 cases). An influence of X-ray treatment is not excluded in 4 cases with tumors in PM.", "contents": "Porokeratosis [correction of Parakeratosis] Mibelli and skin carcinoma: a critical review. The literature on porokeratosis [corrected] Mibelli (PM) is revised critically with respect to the development of skin carcinoma. The incidence of epidermal malignancies in PM lesions is 7% (17 of 250 cases). An influence of X-ray treatment is not excluded in 4 cases with tumors in PM."}
{"id": "PMID:1097327", "title": "Information processing in immunogenetic analysis. V. Some irregularities caused by simple-complex transformation of T universes.", "content": "A fairly uncomplicated T4 universe does give rise to a considerably complicated simple-complex image with several complexities regularly observed together in many immunogenetic systems. The present model thus 'explains' absence of antithetical alleles, absence of 'dosage' effects, presence of considerable quantitative variations of 'one and the same antigen' and peculiar 'non-autoantibodies'. These phenomena are exemplified for the Rh system with regard to the D--Du antigens, absence of d antigens, absence of clear-cut dosage between D/D and D/D and D/d samples as well as anti-D formation in seemingly 'normal Rh(+)' individuals.", "contents": "Information processing in immunogenetic analysis. V. Some irregularities caused by simple-complex transformation of T universes. A fairly uncomplicated T4 universe does give rise to a considerably complicated simple-complex image with several complexities regularly observed together in many immunogenetic systems. The present model thus 'explains' absence of antithetical alleles, absence of 'dosage' effects, presence of considerable quantitative variations of 'one and the same antigen' and peculiar 'non-autoantibodies'. These phenomena are exemplified for the Rh system with regard to the D--Du antigens, absence of d antigens, absence of clear-cut dosage between D/D and D/D and D/d samples as well as anti-D formation in seemingly 'normal Rh(+)' individuals."}
{"id": "PMID:1097331", "title": "In vivo effects of rabbit anti-mouse brain serum on theta bearing lymphocytes of AKR mice.", "content": "The in vivo effect of rabbit anti-AKR mouse brain-associated serum (RAMB) was determined on theta bearing lymphocytes present in the spleens and thymuses of mature C3H mice and AKR mice staged into preleukemic, leukaemic and overtly leukaemic states. Following seven daily injections of RAMB serum, the splenic plaque-forming cell (PFC) response to sheep erythrocytes (SRBC) and the percentage of theta-bearing lymphocytes in the spleen were significantly decreased in the C3H and the preleukaemic AKR mice.  Decreases in thymic weight and thymocyte numbers were also apparent.  Determinations of theta antigen density using in vitro cytoxicity tests indicated that splenic and thymic T lymphocytes (thymus-derived) remaining in the RAMB-treated C3H and preleukemic AKR mice consisted primarily of cells bearing less of the theta surface antigen.  Histopathological studies of tissues from these two treated groups revealed cortical lymphocyte depletion in the thymus, and marginal and periarteriolar depletion in the spleen.  Leukaemic AKR mice, administered seven injections of RAMB serum, demonstrated less dramatic changes in thymus weight, histopathology and theta-bearing cell percentages when compared with the data from the preleukaemic AKR or matureC3H mice.  The results from testing overtly luekaemic AKR mice administered RAMB serum for the 7 or 9 days did not demonstrate differences from findings from groupsof overtly leukaemic control mice.  These data indicate that the in vivo activity of RAMB serum in C3H mice and preleukaemic AKR mice is directed primarily toward the less mature T-lymphocyte population.  This influence of RAMB serum is lesspronounced in leukaemic and the more overtly leukaemic mice, suggesting that a decreasedpopulation of RAMB-susceptible lymphocytes are present in these animals.", "contents": "In vivo effects of rabbit anti-mouse brain serum on theta bearing lymphocytes of AKR mice. The in vivo effect of rabbit anti-AKR mouse brain-associated serum (RAMB) was determined on theta bearing lymphocytes present in the spleens and thymuses of mature C3H mice and AKR mice staged into preleukemic, leukaemic and overtly leukaemic states. Following seven daily injections of RAMB serum, the splenic plaque-forming cell (PFC) response to sheep erythrocytes (SRBC) and the percentage of theta-bearing lymphocytes in the spleen were significantly decreased in the C3H and the preleukaemic AKR mice.  Decreases in thymic weight and thymocyte numbers were also apparent.  Determinations of theta antigen density using in vitro cytoxicity tests indicated that splenic and thymic T lymphocytes (thymus-derived) remaining in the RAMB-treated C3H and preleukemic AKR mice consisted primarily of cells bearing less of the theta surface antigen.  Histopathological studies of tissues from these two treated groups revealed cortical lymphocyte depletion in the thymus, and marginal and periarteriolar depletion in the spleen.  Leukaemic AKR mice, administered seven injections of RAMB serum, demonstrated less dramatic changes in thymus weight, histopathology and theta-bearing cell percentages when compared with the data from the preleukaemic AKR or matureC3H mice.  The results from testing overtly luekaemic AKR mice administered RAMB serum for the 7 or 9 days did not demonstrate differences from findings from groupsof overtly leukaemic control mice.  These data indicate that the in vivo activity of RAMB serum in C3H mice and preleukaemic AKR mice is directed primarily toward the less mature T-lymphocyte population.  This influence of RAMB serum is lesspronounced in leukaemic and the more overtly leukaemic mice, suggesting that a decreasedpopulation of RAMB-susceptible lymphocytes are present in these animals."}
{"id": "PMID:1097332", "title": "Passive transfer of unresponsiveness by lymph node cells.  Studies on adjuvant disease.", "content": "Pretreatment of rats with subarthritogenic doses of mycobacterial adjuvant suppresses the arthritogenic reaction induced by this adjuvant.  This unresponsiveness can betransferred to normal syngeneic recipients by viable lymph node cells from unresponsivedonors.  Serum from the same donors did not prevent the arthritis.  Abrogation ofunresponsiveness was obtained by injecting normal lymph node cells together with the challeging inoculation and also with sensitized lymphoid cells.  In agreement with the concept of tolerance as an active immune reaction, the data suggest that in this experimental model lymphoid cells actively suppress the immune response induced by microbacterial adjuvant.  Thus, arthritis or unresponsiveness may result from the predominance of either sensitized or suppressor cells.", "contents": "Passive transfer of unresponsiveness by lymph node cells.  Studies on adjuvant disease. Pretreatment of rats with subarthritogenic doses of mycobacterial adjuvant suppresses the arthritogenic reaction induced by this adjuvant.  This unresponsiveness can betransferred to normal syngeneic recipients by viable lymph node cells from unresponsivedonors.  Serum from the same donors did not prevent the arthritis.  Abrogation ofunresponsiveness was obtained by injecting normal lymph node cells together with the challeging inoculation and also with sensitized lymphoid cells.  In agreement with the concept of tolerance as an active immune reaction, the data suggest that in this experimental model lymphoid cells actively suppress the immune response induced by microbacterial adjuvant.  Thus, arthritis or unresponsiveness may result from the predominance of either sensitized or suppressor cells."}
{"id": "PMID:1097333", "title": "Complement receptor lymphocytes.  Analysis of immunoglobin on their surface and further evidence of heterogeneity.", "content": "Mouse complement receptor lymphocytes (CRL) were detected by a rosetting method. The existence of an immunoglobulin class or subclass on the central rosetting cell wasstudied by immunofluorescence using rabbit antisera specific for mouse IgM, IgG1, IgG2a, IgG2b and IgA.  Combining immunofluorescence for each immunoglobulin and for T cells with cytoxic removal of T cells, CRL were demonstrated to include immunoglobulin-bearing cells of all the classes and subclasses tested (IgM being the main one), a 'null' cell subpopulation, and some T cells.  A scheme describingthe various nature of CRL is discussed and correlated with possible functions.", "contents": "Complement receptor lymphocytes.  Analysis of immunoglobin on their surface and further evidence of heterogeneity. Mouse complement receptor lymphocytes (CRL) were detected by a rosetting method. The existence of an immunoglobulin class or subclass on the central rosetting cell wasstudied by immunofluorescence using rabbit antisera specific for mouse IgM, IgG1, IgG2a, IgG2b and IgA.  Combining immunofluorescence for each immunoglobulin and for T cells with cytoxic removal of T cells, CRL were demonstrated to include immunoglobulin-bearing cells of all the classes and subclasses tested (IgM being the main one), a 'null' cell subpopulation, and some T cells.  A scheme describingthe various nature of CRL is discussed and correlated with possible functions."}
{"id": "PMID:1097334", "title": "Electrophoretic mobility and surface immunoglobin of albumin gradient fractionated mouse spleen cells.", "content": "Spleen cells from normal CBA mice, containing B and T lymphocytes, cyclophosphamide-treated CBA mice, containing almost exclusively T lymphocytes, and athymic nudemice, containing only B lymphocytes, were fracitonated by differential flotation in adiscontinuous albumin gradient.  In all three cases, four density fractions were regularly obtained.  The electrophoretic mobility (EPM), which allows distinction betweenslow-EPM (B) cells and fast-EPM (T) cells and the presence of surface immunoglobulins (sIg) detectable by direct immunofluorescence, characterizing B lymphocytes, were investigated on these fractions.  Both B and T cells were recovered throughout the gradient, but in different proportions.  Thus, B cells (slow-EPM, sIg-bearing) were enriched in the light density fractions while T cells (fast-EPM)were more numerous in the denser fraction.  The mean EPM of slow-moving cells decreased, whereas that of fast-moving cells increased, as their buoyant density increased.  Less nude spleen (B) cells were found to bear sIg in the light density fractions than in the denser fractions.  These findings suggest the existence of lymphocyte subpopulations with distinct physicochemical properties which might represent stages in the maturation and differentiation of B- and T-cell lineages.", "contents": "Electrophoretic mobility and surface immunoglobin of albumin gradient fractionated mouse spleen cells. Spleen cells from normal CBA mice, containing B and T lymphocytes, cyclophosphamide-treated CBA mice, containing almost exclusively T lymphocytes, and athymic nudemice, containing only B lymphocytes, were fracitonated by differential flotation in adiscontinuous albumin gradient.  In all three cases, four density fractions were regularly obtained.  The electrophoretic mobility (EPM), which allows distinction betweenslow-EPM (B) cells and fast-EPM (T) cells and the presence of surface immunoglobulins (sIg) detectable by direct immunofluorescence, characterizing B lymphocytes, were investigated on these fractions.  Both B and T cells were recovered throughout the gradient, but in different proportions.  Thus, B cells (slow-EPM, sIg-bearing) were enriched in the light density fractions while T cells (fast-EPM)were more numerous in the denser fraction.  The mean EPM of slow-moving cells decreased, whereas that of fast-moving cells increased, as their buoyant density increased.  Less nude spleen (B) cells were found to bear sIg in the light density fractions than in the denser fractions.  These findings suggest the existence of lymphocyte subpopulations with distinct physicochemical properties which might represent stages in the maturation and differentiation of B- and T-cell lineages."}
{"id": "PMID:1097335", "title": "Heat-stable Escherichia coli enterotoxin production in vivo.", "content": "Hysterectomy-derived, colostrum-deprived piglets were infected with enterotoxigenic Escherichia coli on day 4 of life. Samples of feces and intestinal contents were collected and tested in infant mice for enterotoxic activity. Positive enterotoxic responses were observed in mice given filtrates of feces and intestinal contents from piglets infected withe enterotoxigenic E. coli known to produce heat-stable enterotoxin but not heat-liabile enterotoxin in vitro. It is concluded that heat-stable enterotoxigenic E. coli induce diarrhea by production of heat-stable enterotoxin in vivo.", "contents": "Heat-stable Escherichia coli enterotoxin production in vivo. Hysterectomy-derived, colostrum-deprived piglets were infected with enterotoxigenic Escherichia coli on day 4 of life. Samples of feces and intestinal contents were collected and tested in infant mice for enterotoxic activity. Positive enterotoxic responses were observed in mice given filtrates of feces and intestinal contents from piglets infected withe enterotoxigenic E. coli known to produce heat-stable enterotoxin but not heat-liabile enterotoxin in vitro. It is concluded that heat-stable enterotoxigenic E. coli induce diarrhea by production of heat-stable enterotoxin in vivo."}
{"id": "PMID:1097336", "title": "Cellular location of alpha-hemolysin in Staphylococcus aureus.", "content": "Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.", "contents": "Cellular location of alpha-hemolysin in Staphylococcus aureus. Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form."}
{"id": "PMID:1097337", "title": "Prevention of Streptococcus mutans infection of tooth surfaces by salivary antibody in Irus monkeys (Macaca fascicularis).", "content": "Four irus monkeys (Macaca fascicularis) were immunized with Streptococcus mutans 6715 killed cells and cell products by injection in the vicinity of the major salivary glands and by instillation into the parotid glands via the ducts. After immune group and a sham-immunized control group of monkeys were infected orally with viable strain 6715 organisms. Dental plaque samples were taken at intervals from the buccal and lingual grooves of the first permanent molars. These samples were evaluated for recovery of strain 6715 by cultural methods. In addition, individual samples were taken from 10 representative tooth surfaces and were evaluated by indirect immunofluorescent staining for strain 6715. Results showed that immune monkeys had fewer infected surfaces and fewer organisms on the infected surfaces than the control animals. These studies indicate that salivary antibody to cariogenic streptococci inhibits implantation of these organisms in dental plaque and may be protective against dental caries.", "contents": "Prevention of Streptococcus mutans infection of tooth surfaces by salivary antibody in Irus monkeys (Macaca fascicularis). Four irus monkeys (Macaca fascicularis) were immunized with Streptococcus mutans 6715 killed cells and cell products by injection in the vicinity of the major salivary glands and by instillation into the parotid glands via the ducts. After immune group and a sham-immunized control group of monkeys were infected orally with viable strain 6715 organisms. Dental plaque samples were taken at intervals from the buccal and lingual grooves of the first permanent molars. These samples were evaluated for recovery of strain 6715 by cultural methods. In addition, individual samples were taken from 10 representative tooth surfaces and were evaluated by indirect immunofluorescent staining for strain 6715. Results showed that immune monkeys had fewer infected surfaces and fewer organisms on the infected surfaces than the control animals. These studies indicate that salivary antibody to cariogenic streptococci inhibits implantation of these organisms in dental plaque and may be protective against dental caries."}
{"id": "PMID:1097338", "title": "Antibody-mediated elimination of malaria parasites (plasmodium berghei) in vivo.", "content": "An infective preparation of extracellular blood forms (FP) of Plasmodium berghei was used to study some aspects of the interaction between protective antibodies and malaria parasites. FP but not infected erythrocytes (IRBC) were shown by the fluorescent antibody technique to be coated by antibodies after in vitro incubation with immune serum. Preincubation of both FP and IRBC with immune serum followed by their washing did not result in enhanced elimination of the parasites in vivo. However, FP preincubated with immune serum and subsequently washed were eliminated more efficiently than FP preincubated with normal serum if the preparations were injected with some immune serum. Such an increase in the efficiency of elimination was not detected with similarly pretreated IRBC. It is thus probable that protective antibodies acted in vivo against extracellular parasites rather than against parasites in erythrocytes. The interaction between parasites and antibodies may be of a highly reversible nature, and washing of the in vitro-treated parasites may cause elution of antibody from the sensitized parasites so that the amount of antibody on the parasite falls below the critical level required for in vivo elimination.", "contents": "Antibody-mediated elimination of malaria parasites (plasmodium berghei) in vivo. An infective preparation of extracellular blood forms (FP) of Plasmodium berghei was used to study some aspects of the interaction between protective antibodies and malaria parasites. FP but not infected erythrocytes (IRBC) were shown by the fluorescent antibody technique to be coated by antibodies after in vitro incubation with immune serum. Preincubation of both FP and IRBC with immune serum followed by their washing did not result in enhanced elimination of the parasites in vivo. However, FP preincubated with immune serum and subsequently washed were eliminated more efficiently than FP preincubated with normal serum if the preparations were injected with some immune serum. Such an increase in the efficiency of elimination was not detected with similarly pretreated IRBC. It is thus probable that protective antibodies acted in vivo against extracellular parasites rather than against parasites in erythrocytes. The interaction between parasites and antibodies may be of a highly reversible nature, and washing of the in vitro-treated parasites may cause elution of antibody from the sensitized parasites so that the amount of antibody on the parasite falls below the critical level required for in vivo elimination."}
{"id": "PMID:1097339", "title": "Days beyond recall: subsistence homesteading in the rural south, circa 1920.", "content": "Accented in some 100 semi-structured interviews with Southern aged poor is the bitter struggle to survive. People worked long hours for little, bartering farm produce for the few store-bought necessities. Primitive housing, education and medicine sufficed. Both black and white poor suffered brutal exploitation, despite which they review their life with satisfaction. We hypothesize that, to maintain mental health, it is better to idealize the unalterable past. Several global assessments of \"morale\" are discussed. It is also suggested that this journal become a clearing house for questions that measure psychic wellbeing in the aged more naturally and by indirection than do scales in current use.", "contents": "Days beyond recall: subsistence homesteading in the rural south, circa 1920. Accented in some 100 semi-structured interviews with Southern aged poor is the bitter struggle to survive. People worked long hours for little, bartering farm produce for the few store-bought necessities. Primitive housing, education and medicine sufficed. Both black and white poor suffered brutal exploitation, despite which they review their life with satisfaction. We hypothesize that, to maintain mental health, it is better to idealize the unalterable past. Several global assessments of \"morale\" are discussed. It is also suggested that this journal become a clearing house for questions that measure psychic wellbeing in the aged more naturally and by indirection than do scales in current use."}
{"id": "PMID:1097340", "title": "Model of an oculomotor subsystem.", "content": "One of the complex control systems present in the human body is the oculomotor control system. There are two major modes of ocular movements, viz. saccadic and smooth pursuit. More knowledge about the neuromuscular mechanisms controlling these eye movements is obtained if experiments are carried out on subjects whose two types of eye movements are differentially affected due to disorders at their higher centres of control. Eye movements of patients with a novel form of Heredo-familial Spinocerebellar Degeneration have been measured and quantified. These have been compared with those of normal subjects. Subsequent analysis indicates a continuous fourth order transfer function for the smooth pursuit eye-in-skull movements in the horizontal plane with head stationary.", "contents": "Model of an oculomotor subsystem. One of the complex control systems present in the human body is the oculomotor control system. There are two major modes of ocular movements, viz. saccadic and smooth pursuit. More knowledge about the neuromuscular mechanisms controlling these eye movements is obtained if experiments are carried out on subjects whose two types of eye movements are differentially affected due to disorders at their higher centres of control. Eye movements of patients with a novel form of Heredo-familial Spinocerebellar Degeneration have been measured and quantified. These have been compared with those of normal subjects. Subsequent analysis indicates a continuous fourth order transfer function for the smooth pursuit eye-in-skull movements in the horizontal plane with head stationary."}
{"id": "PMID:1097341", "title": "An algorithm for on-line, real-time computer detection of ECG changes.", "content": "An algorithm for detection of ECG changes based on the Fourier transform is presented. The realisation of the algorithm was programmed on a minicomputer and could detect both large and subtle changes in an ECG waveform. The cardiac arrhythmias used in the study included normal sinus rhythm; premature atrial contraction; premature ventricular contraction; first, second, and third degree blocks; atrial and ventricular fibrillation and ventricular tachycardia. The algorithm was used to detect rhythm changes, but it is possible that, using it, a unique specification for a waveform at any frequency could be obtained.", "contents": "An algorithm for on-line, real-time computer detection of ECG changes. An algorithm for detection of ECG changes based on the Fourier transform is presented. The realisation of the algorithm was programmed on a minicomputer and could detect both large and subtle changes in an ECG waveform. The cardiac arrhythmias used in the study included normal sinus rhythm; premature atrial contraction; premature ventricular contraction; first, second, and third degree blocks; atrial and ventricular fibrillation and ventricular tachycardia. The algorithm was used to detect rhythm changes, but it is possible that, using it, a unique specification for a waveform at any frequency could be obtained."}
{"id": "PMID:1097342", "title": "Temperature-sensitive mutants of murine leukemia virus. V. Impaired leukemogenic activity in vivo.", "content": "The in vivo biologic activities of a clonal isolate of the Rauscher strain of murine leukemia virus and several conditional lethal mutants with impaired replicative functions at the non-permissive temperature were investigated. The parental virus induced a rapidly developing lymphoid leukemia in a high percentage of NIH Swiss mice inoculated as newborns. Mice inoculated in parallel with temperature-sensitive mutant viruses showed no evidence of disease. The implications of these findings with regard to the requirements for type-C virus replication to permit oncogenic transformation of lymphoid cells in vivo are discussed.", "contents": "Temperature-sensitive mutants of murine leukemia virus. V. Impaired leukemogenic activity in vivo. The in vivo biologic activities of a clonal isolate of the Rauscher strain of murine leukemia virus and several conditional lethal mutants with impaired replicative functions at the non-permissive temperature were investigated. The parental virus induced a rapidly developing lymphoid leukemia in a high percentage of NIH Swiss mice inoculated as newborns. Mice inoculated in parallel with temperature-sensitive mutant viruses showed no evidence of disease. The implications of these findings with regard to the requirements for type-C virus replication to permit oncogenic transformation of lymphoid cells in vivo are discussed."}
{"id": "PMID:1097343", "title": "Comparative clinical study of doxycycline and minocycline.", "content": "Doxycycline and minocycline were given intravenously to patients with a serious underlying disease who presented a pulmonary infection or a wound infection. Both therapies were easy to administer and well tolerated. However they should not be used in the initial therapy since resistant strains may occur. The clinical and bacteriological effectiveness of doxycycline and minocycline were similar. In pneumococcal pulmonary infections, the rates of favorable clinical response to doxycycline and minocycline were 73% and 76% respectively. In infections caused by bacteroides sp.(mainly infections of wounds), doxycycline or minocycline resulted in a 75% rate of favorable clinical responses and in a 71% rate of favorable bacteriological responses. Adverse side effects were rare and of minor importance with the exception of bacteriological colonization by doxycycline-resistant or minocycline-resistant microorganisms. This complica-tion occurred in 39% of the patients who were treated with these drugs and resulted in clinical superinfection in 8% of the patients.", "contents": "Comparative clinical study of doxycycline and minocycline. Doxycycline and minocycline were given intravenously to patients with a serious underlying disease who presented a pulmonary infection or a wound infection. Both therapies were easy to administer and well tolerated. However they should not be used in the initial therapy since resistant strains may occur. The clinical and bacteriological effectiveness of doxycycline and minocycline were similar. In pneumococcal pulmonary infections, the rates of favorable clinical response to doxycycline and minocycline were 73% and 76% respectively. In infections caused by bacteroides sp.(mainly infections of wounds), doxycycline or minocycline resulted in a 75% rate of favorable clinical responses and in a 71% rate of favorable bacteriological responses. Adverse side effects were rare and of minor importance with the exception of bacteriological colonization by doxycycline-resistant or minocycline-resistant microorganisms. This complica-tion occurred in 39% of the patients who were treated with these drugs and resulted in clinical superinfection in 8% of the patients."}
{"id": "PMID:1097344", "title": "[The influence of Adipex Neu (beta-aminoisobutylbenzene as polysterol sulfonate) on lipolysis and glucose tolerance in humans].", "content": "In 16 obese patients, the effect of beta-amino-isobutyl-benzol as polysterol-sulfonate on free fatty acids, triglycerides and i.v. GTT was compaired with that of a placebo. The question was to be answered if beta-amino-isobutyl-benzol worsens the GTT and if the lipolytic effect outranges lipolysis during fasting. No statistically significant change of the GTT, free fatty acids and triglycerides could be found between the beta-amino-isobutyl-benzol period and the placebo period.", "contents": "[The influence of Adipex Neu (beta-aminoisobutylbenzene as polysterol sulfonate) on lipolysis and glucose tolerance in humans]. In 16 obese patients, the effect of beta-amino-isobutyl-benzol as polysterol-sulfonate on free fatty acids, triglycerides and i.v. GTT was compaired with that of a placebo. The question was to be answered if beta-amino-isobutyl-benzol worsens the GTT and if the lipolytic effect outranges lipolysis during fasting. No statistically significant change of the GTT, free fatty acids and triglycerides could be found between the beta-amino-isobutyl-benzol period and the placebo period."}
{"id": "PMID:1097345", "title": "Double-blind study of a total liver extract in patients with hepatic dysfunction.", "content": "Forty hospitalized male patients with hepatic functional deficit were treated i.m. for 3 weeks with a total liver extract or placebo. The study was a double-blind. Statistical analysis (Student's t test, analysis of variance. Mann-Whitney test and Fisher's probability test) showed that liver extract therapy was more effective than placebo in improving liver function as indicated by changes in clinical features(digestive disorders, constipation, hepatomegaly) and colloidal and other laboratory tests, e.g. plasma cholesterol, prothrombin time, red cell count and hemoglobin concentration. An overall clinical assessment showed that 60% of patients treated with the liver extract were improved. In addition, no local or general side effects were observed. Present results are in favour of an hepatoprotective activity of a total liver extract.", "contents": "Double-blind study of a total liver extract in patients with hepatic dysfunction. Forty hospitalized male patients with hepatic functional deficit were treated i.m. for 3 weeks with a total liver extract or placebo. The study was a double-blind. Statistical analysis (Student's t test, analysis of variance. Mann-Whitney test and Fisher's probability test) showed that liver extract therapy was more effective than placebo in improving liver function as indicated by changes in clinical features(digestive disorders, constipation, hepatomegaly) and colloidal and other laboratory tests, e.g. plasma cholesterol, prothrombin time, red cell count and hemoglobin concentration. An overall clinical assessment showed that 60% of patients treated with the liver extract were improved. In addition, no local or general side effects were observed. Present results are in favour of an hepatoprotective activity of a total liver extract."}
{"id": "PMID:1097347", "title": "Herpetiform pemphigus, a variable pattern of pemphigus.", "content": "A  patient with the clinical features of dermatitis herpetiformis and the histological features of pemphigus, subcorneal dermatosis and dermatitis herpetiformis did not respond to treatment with pyridine or sulfones. Systemic steroids and azothioprine were effective. Immunofluorescence studies demonstrated circulating pemphigus antibodies and IgG bound in vivo in the intercellular spaces of the epidermis.", "contents": "Herpetiform pemphigus, a variable pattern of pemphigus. A  patient with the clinical features of dermatitis herpetiformis and the histological features of pemphigus, subcorneal dermatosis and dermatitis herpetiformis did not respond to treatment with pyridine or sulfones. Systemic steroids and azothioprine were effective. Immunofluorescence studies demonstrated circulating pemphigus antibodies and IgG bound in vivo in the intercellular spaces of the epidermis."}
{"id": "PMID:1097351", "title": "Development of the upper urinary tract in myelomeningocele and consequent urological care.", "content": "The findings in the upper urinary tract of 58 patients with MMC and neurogenic bladder dysfunction are discussed and therapeutical consequences are considered. In a group of 28 neonates, investigated within the first three months of life, only 10% showed some abnormalities of the upper urinary tract and (or) reflux, in a second group of 21 children, over the age of 2 years when first seen, the occurrence rate of dilatation of the upper urinary tract and of pyelonephritis signs was already doubled, whereas adults with operated MMC showed in 75% severe pathological changes. The situation of neonates born with MMC may be in some aspects compared to patients suffering from acute traumatic cord lesion with neurogenic bladder dysfunction. It was already shown that, in spite of neurogenic bladder dysfunction, it is possible in parapegics by appropiate and mainly conservative treatment to prevent progressive destruction of the upper urinary tract, once considered to be unavoidable. The key to better results in the children with MMC should therefore be early and prompt care and close follow-up. The importance of regular bladder emptying, low dosage, long-term antibiotic treatment and measures like forced urethral dilatation and sphincterotomy is stressed. Our results so far in neonatal urological care of children with MMC are encouraging.", "contents": "Development of the upper urinary tract in myelomeningocele and consequent urological care. The findings in the upper urinary tract of 58 patients with MMC and neurogenic bladder dysfunction are discussed and therapeutical consequences are considered. In a group of 28 neonates, investigated within the first three months of life, only 10% showed some abnormalities of the upper urinary tract and (or) reflux, in a second group of 21 children, over the age of 2 years when first seen, the occurrence rate of dilatation of the upper urinary tract and of pyelonephritis signs was already doubled, whereas adults with operated MMC showed in 75% severe pathological changes. The situation of neonates born with MMC may be in some aspects compared to patients suffering from acute traumatic cord lesion with neurogenic bladder dysfunction. It was already shown that, in spite of neurogenic bladder dysfunction, it is possible in parapegics by appropiate and mainly conservative treatment to prevent progressive destruction of the upper urinary tract, once considered to be unavoidable. The key to better results in the children with MMC should therefore be early and prompt care and close follow-up. The importance of regular bladder emptying, low dosage, long-term antibiotic treatment and measures like forced urethral dilatation and sphincterotomy is stressed. Our results so far in neonatal urological care of children with MMC are encouraging."}
{"id": "PMID:1097352", "title": "Pyleonphritis as a side effect of hormonal contraception: an experimental research.", "content": "In animal experiments it was proved that contraceptives may cause considerable renal changes. It is necessary to call gynecologists' attention to possible side effects; the contrapaceptives are contra-indicated in chronic pyelonephritis.", "contents": "Pyleonphritis as a side effect of hormonal contraception: an experimental research. In animal experiments it was proved that contraceptives may cause considerable renal changes. It is necessary to call gynecologists' attention to possible side effects; the contrapaceptives are contra-indicated in chronic pyelonephritis."}
{"id": "PMID:1097350", "title": "Experimental heterotopic bone transplant.", "content": "We studied the reaction in rats to implants of heterotopic bone in the paravertebral region, using three types of material: bone preserved in alcohol, homologous bone preserved in alcohol associated with autogenous cancellous bone obtained from the iliac crest of the same animal, and isolated autogenous cancellous bone. Thirty rats were operated on, ten in each group. Some were sacrificed after 6, 14 and 21 days. The excised grafts were histologically studied. Animals in the third group showed new bone formation even in the first days, those in the second group presented less intense bone formation and those in the first group showed only traces of new bone tissue in the third week.", "contents": "Experimental heterotopic bone transplant. We studied the reaction in rats to implants of heterotopic bone in the paravertebral region, using three types of material: bone preserved in alcohol, homologous bone preserved in alcohol associated with autogenous cancellous bone obtained from the iliac crest of the same animal, and isolated autogenous cancellous bone. Thirty rats were operated on, ten in each group. Some were sacrificed after 6, 14 and 21 days. The excised grafts were histologically studied. Animals in the third group showed new bone formation even in the first days, those in the second group presented less intense bone formation and those in the first group showed only traces of new bone tissue in the third week."}
{"id": "PMID:1097361", "title": "Optic nerve transection in cats: effect on retinal vessels.", "content": "Unilateral optic nerve transection without damage to the intraocular circulation was performed on thirteen cats. Fluorescein angiograms, trypsin digestion, and histologic preparation of the retinas were carried out. No changes in the retinal circulation and angioarchitecture were observed. These findings were compared to those reported in humans with comparable optic nerve lesions. We conclude that optic nerve transection does not cause retinal vascular alteration and this fact may be of pertinence to posterior ocular damage in glaucoma.", "contents": "Optic nerve transection in cats: effect on retinal vessels. Unilateral optic nerve transection without damage to the intraocular circulation was performed on thirteen cats. Fluorescein angiograms, trypsin digestion, and histologic preparation of the retinas were carried out. No changes in the retinal circulation and angioarchitecture were observed. These findings were compared to those reported in humans with comparable optic nerve lesions. We conclude that optic nerve transection does not cause retinal vascular alteration and this fact may be of pertinence to posterior ocular damage in glaucoma."}
{"id": "PMID:1097371", "title": "[Keratohyaline and epidermal keratihization].", "content": "A review is given about the present status of keratohyalin, his composition and his origin and also about \"prekeratin\". The suggestions about the role of keratohyalin and prekeratin in the synthesis of epidermal horny layer are discussed.", "contents": "[Keratohyaline and epidermal keratihization]. A review is given about the present status of keratohyalin, his composition and his origin and also about \"prekeratin\". The suggestions about the role of keratohyalin and prekeratin in the synthesis of epidermal horny layer are discussed."}
{"id": "PMID:1097379", "title": "Comparison of the reaction of cultured smooth and cardiac muscle cells and fibroblasts to specific antibodies to myosin.", "content": "Immunofluorescent staining with anti-smooth or anti-striated muscle myosin was carried out for 30 minutes at room temperature (18-20 degrees C) on cultures of smooth muscle cells and fibroblasts from guinea-pig vas deferens, taenia coli and ureter, rabbit aorta and chicken gizzard and of cardiac muscle cells and fibroblasts from rat ventricle. With anti-smooth muscle myosin, smooth muscle cells showed an intense fluorescent staining in fine fibrils with an \"interrupted\" appearance running parallel to the longitudinal axis of the cell throughout the cytoplasm, and also in coarser, \"non-interrupted\" fibrils (termed here \"attachment fibrils\") concentrated at the surface of the cell adjacent to the glass coverslip. Fibroblasts in the same cultures showed similar, but much weaker, reactions. When anti-striated myosin was added to the smooth muscle cultures, staining of neither cell type was observed. In contrast, cardiac muscle cells in cultures of rat ventricle did not react anti-smooth muscle myosin, but gave bright fluorescent A-band staining with anti-striated myosin. Fibroblasts in the ventricle cultures were unreactive with anti-striated muscle myosin but gave the characteristic weak reaction with anti-smooth muscle myosin. Thus immunofluorescent stainig with anti-smooth muscle myosin is useful for distinguishing between isolated smooth muscle cells and fibroblasts in tissue culture.", "contents": "Comparison of the reaction of cultured smooth and cardiac muscle cells and fibroblasts to specific antibodies to myosin. Immunofluorescent staining with anti-smooth or anti-striated muscle myosin was carried out for 30 minutes at room temperature (18-20 degrees C) on cultures of smooth muscle cells and fibroblasts from guinea-pig vas deferens, taenia coli and ureter, rabbit aorta and chicken gizzard and of cardiac muscle cells and fibroblasts from rat ventricle. With anti-smooth muscle myosin, smooth muscle cells showed an intense fluorescent staining in fine fibrils with an \"interrupted\" appearance running parallel to the longitudinal axis of the cell throughout the cytoplasm, and also in coarser, \"non-interrupted\" fibrils (termed here \"attachment fibrils\") concentrated at the surface of the cell adjacent to the glass coverslip. Fibroblasts in the same cultures showed similar, but much weaker, reactions. When anti-striated myosin was added to the smooth muscle cultures, staining of neither cell type was observed. In contrast, cardiac muscle cells in cultures of rat ventricle did not react anti-smooth muscle myosin, but gave bright fluorescent A-band staining with anti-striated myosin. Fibroblasts in the ventricle cultures were unreactive with anti-striated muscle myosin but gave the characteristic weak reaction with anti-smooth muscle myosin. Thus immunofluorescent stainig with anti-smooth muscle myosin is useful for distinguishing between isolated smooth muscle cells and fibroblasts in tissue culture."}
{"id": "PMID:1097380", "title": "Identification of the intestinal cell storing gastric inhibitory peptide.", "content": "Small intestinal mucosal samples from man, pig and dog, were subjected to sequential or correlative silver impregnation techniques, applied to immunocytochemical preparations and at the ultrastructural level. The cell reacting with anti-GIP sera was identified as the ultrastructurally classified K cell and we propose that the term GIP cell be used in place of the latter. This cell can thus be recognized by its strong reactivity with the Sevier-Munger staining procedure, provided that the equally strongly reacting EC cell is excluded by virtue of its argentaffinity with the Masson technique.", "contents": "Identification of the intestinal cell storing gastric inhibitory peptide. Small intestinal mucosal samples from man, pig and dog, were subjected to sequential or correlative silver impregnation techniques, applied to immunocytochemical preparations and at the ultrastructural level. The cell reacting with anti-GIP sera was identified as the ultrastructurally classified K cell and we propose that the term GIP cell be used in place of the latter. This cell can thus be recognized by its strong reactivity with the Sevier-Munger staining procedure, provided that the equally strongly reacting EC cell is excluded by virtue of its argentaffinity with the Masson technique."}
{"id": "PMID:1097381", "title": "A field study of coliform mastitis in sows.", "content": "Three sows with agalactia and mastitis were compared with 3 normally lactating sows at 8, 16, 24, and 32 hours postpartum. Bacteriologic examination of milk resulted in isolation of Klebsiella pneumoniae or Escherichia coli from affected glands of all 3 agalactic sows. Milk from clinically normal glands of affected sows and from glands of normal sows was culture-negative for coliforms. Affected sows had higher rectal temperatures and lower milk yields, and pigs from affected sows had lower mean weights, but these differences were not significant. Mean total white blood cell counts and plasma protein to fibrinogen ratios were lower in mastitic sows at all 4 periods; differences were significant at certain periods in both cases. Evidence indicated that leukopenia appeared early, 8 to 16 hours postpartum, and was followed by increasing plasma fibrinogen content. Packed cell volumes and plasma concentrations of corticoid, estrone, and estradiol did not differ. Plasma progesterone content was higher in mastitic sows at all periods, but the differences were not significant.", "contents": "A field study of coliform mastitis in sows. Three sows with agalactia and mastitis were compared with 3 normally lactating sows at 8, 16, 24, and 32 hours postpartum. Bacteriologic examination of milk resulted in isolation of Klebsiella pneumoniae or Escherichia coli from affected glands of all 3 agalactic sows. Milk from clinically normal glands of affected sows and from glands of normal sows was culture-negative for coliforms. Affected sows had higher rectal temperatures and lower milk yields, and pigs from affected sows had lower mean weights, but these differences were not significant. Mean total white blood cell counts and plasma protein to fibrinogen ratios were lower in mastitic sows at all 4 periods; differences were significant at certain periods in both cases. Evidence indicated that leukopenia appeared early, 8 to 16 hours postpartum, and was followed by increasing plasma fibrinogen content. Packed cell volumes and plasma concentrations of corticoid, estrone, and estradiol did not differ. Plasma progesterone content was higher in mastitic sows at all periods, but the differences were not significant."}
{"id": "PMID:1097382", "title": "Bovine infectious keratoconjunctivitis: carrier state of Moraxella bovis and the development of preventive measures against disease.", "content": "Bovine infectious keratoconjunctivitis, which is caused by Moraxella bovis, occurs perennially in all cattle-raising areas. Disease may occur any time during the year but manifests itself often during summer when disease-enhancing factors such as ultraviolet radiation and fly population are increased. Although some recent research findings indicate that cattle can be immunized against the disease, many problems have to be overcome before the cattle industry can benefit from such immunization. Some pressing problems relate to lack of cross-protection between vaccines of different strains of M bovis, resistant or resilient carrier states, and how these factors affect vaccination regimens undertaken. Though cattle vaccinated with 1 strain are somewhat protected against subsequent challange by the same strain, protection against heterologous strains has not been demonstrated. In most herds, cattle harbor M bovis, and these carriers may serve as a source of infection.", "contents": "Bovine infectious keratoconjunctivitis: carrier state of Moraxella bovis and the development of preventive measures against disease. Bovine infectious keratoconjunctivitis, which is caused by Moraxella bovis, occurs perennially in all cattle-raising areas. Disease may occur any time during the year but manifests itself often during summer when disease-enhancing factors such as ultraviolet radiation and fly population are increased. Although some recent research findings indicate that cattle can be immunized against the disease, many problems have to be overcome before the cattle industry can benefit from such immunization. Some pressing problems relate to lack of cross-protection between vaccines of different strains of M bovis, resistant or resilient carrier states, and how these factors affect vaccination regimens undertaken. Though cattle vaccinated with 1 strain are somewhat protected against subsequent challange by the same strain, protection against heterologous strains has not been demonstrated. In most herds, cattle harbor M bovis, and these carriers may serve as a source of infection."}
{"id": "PMID:1097383", "title": "Antibiotic A-16316-C, a new water-soluble basic antibiotic.", "content": "A new water-soluble basic antibiotic named antibiotic A-16316-C was isolated together with antibiotic A-396-I and hygromycin B from a streptomyces strain identified as Streptoverticullium eurocidicus.  The properties of the antibiotic A-16316-C were similar to those of destomycin B.  But it was found that the antibiotic A-16316-C was not identical with destomycin B on the basis of NMR analysis.  On acidic degradation antibiotic A-16136-C gave N, N'-dimethyl-2-deoxystreptamine, destomic acid and D-mannose.  The gross structure for antibiotic A-16136-C was deduced from chemical reactions and spectral data.", "contents": "Antibiotic A-16316-C, a new water-soluble basic antibiotic. A new water-soluble basic antibiotic named antibiotic A-16316-C was isolated together with antibiotic A-396-I and hygromycin B from a streptomyces strain identified as Streptoverticullium eurocidicus.  The properties of the antibiotic A-16316-C were similar to those of destomycin B.  But it was found that the antibiotic A-16316-C was not identical with destomycin B on the basis of NMR analysis.  On acidic degradation antibiotic A-16136-C gave N, N'-dimethyl-2-deoxystreptamine, destomic acid and D-mannose.  The gross structure for antibiotic A-16136-C was deduced from chemical reactions and spectral data."}
{"id": "PMID:1097384", "title": "Inhibitors of polypeptide elongation on yeast polysomes.", "content": "Yeast polysomes are very active for amino acid incorporation when supplemented with elongation factors and the different components required for elongation of the polypeptide chain. This polysomal system is suitable for the study of the individual streps of the elongation cycle and to test the effect of different inhibitors. Anisomycin, trichodermin, trichodermol, trichothecin, fusarenon X, sparsomycin and blasticidin S inhibit peptide bond formation on these polysomes, whereas diphtheria toxin, pederine, cycloheximide and cryptopleurine block translocation.", "contents": "Inhibitors of polypeptide elongation on yeast polysomes. Yeast polysomes are very active for amino acid incorporation when supplemented with elongation factors and the different components required for elongation of the polypeptide chain. This polysomal system is suitable for the study of the individual streps of the elongation cycle and to test the effect of different inhibitors. Anisomycin, trichodermin, trichodermol, trichothecin, fusarenon X, sparsomycin and blasticidin S inhibit peptide bond formation on these polysomes, whereas diphtheria toxin, pederine, cycloheximide and cryptopleurine block translocation."}
{"id": "PMID:1097385", "title": "Surfactant inactivation by hyperventilation: conservation by end-expiratory pressure.", "content": "Hyperventilation, defined as repeated hyperinflations, for three hours in open-chested anesthetized cats increased elastic recoil and elevated minimum surface tension of lung extracts as measured on a surface film balance. Equivalent hyperventilation from an elevated lung volume did not alter the pressure-volume relationships. When a mixture of [3H]glycerol and [14C]palmitate had been injected 17 h before the three hour period of phyerventilation, an increase in the ratio of specific activity in wash to tissue lecithin occurred in the hyperventilated cats compared to controls. These findings suggest that hyperventilation promotes release of surface active material from tissue to alveolus, but the released material is inactivated. The application of 2.5 cmH2O positive end-expiratory pressure prevented the adverse effects of hyperventilation. The same increase in wash to tissue lecithin occurred during this study; since the material was appropriately surface active, we conclude that the positive end-expiratory pressure prevented its inactivation.", "contents": "Surfactant inactivation by hyperventilation: conservation by end-expiratory pressure. Hyperventilation, defined as repeated hyperinflations, for three hours in open-chested anesthetized cats increased elastic recoil and elevated minimum surface tension of lung extracts as measured on a surface film balance. Equivalent hyperventilation from an elevated lung volume did not alter the pressure-volume relationships. When a mixture of [3H]glycerol and [14C]palmitate had been injected 17 h before the three hour period of phyerventilation, an increase in the ratio of specific activity in wash to tissue lecithin occurred in the hyperventilated cats compared to controls. These findings suggest that hyperventilation promotes release of surface active material from tissue to alveolus, but the released material is inactivated. The application of 2.5 cmH2O positive end-expiratory pressure prevented the adverse effects of hyperventilation. The same increase in wash to tissue lecithin occurred during this study; since the material was appropriately surface active, we conclude that the positive end-expiratory pressure prevented its inactivation."}
{"id": "PMID:1097386", "title": "A new method for calibrating indicator dilution curves.", "content": "A technique is proposed and experiments carried out to validate an in vivo method of calibrating ascorbate dilution curves. Cardiac output measured by the proposed method (COi) was compared to cardiac output measured by electromagnetic flowmeter (COe). COe averaged 1,281 mg/min and COi averaged 1,284 ml/min (P greater than 0.90). Correlation between the two methods was 0.92. It is concluded that the proposed method measures CO with reasonable accuracy and provides a number of advantages over the usual method of measuring blood flow by means of indicators.", "contents": "A new method for calibrating indicator dilution curves. A technique is proposed and experiments carried out to validate an in vivo method of calibrating ascorbate dilution curves. Cardiac output measured by the proposed method (COi) was compared to cardiac output measured by electromagnetic flowmeter (COe). COe averaged 1,281 mg/min and COi averaged 1,284 ml/min (P greater than 0.90). Correlation between the two methods was 0.92. It is concluded that the proposed method measures CO with reasonable accuracy and provides a number of advantages over the usual method of measuring blood flow by means of indicators."}
{"id": "PMID:1097387", "title": "Fluorescent antibody method for the detection of Salmonella: development, evaluation, and collaborative study.", "content": "In preliminary studies, several commercial polyvalent fluorescent antibody (FA) preparations were evaluated for specificity and crossreactivity and an FA method was developed for the screening of Salmonella in products. Approximately 4000 product samples were tested by the FA method and the results were compared to those from the official final action AOAC method, 46.013-46.026. Only 4 FA false-negatives were found for a total of 619 confirmed positive Salmonella samples. The FA false-positive rate was 7%. The method was then subjected to a 2-phase collaborative study. In Phase I, 22 analysts tested 5 inoculated and 5 uninoculated samples of dried milk. In Phase II, 5 naturally contaminated and 5 presumably uncontaminated foods were analyzed. The study was designed to compare results from 11 analysts experienced in FA methodology with those from 11 analysts with little or no experience. Selenite cystine (SC) and tetrathionate (TT) broths were used for enrichment and both were inoculated into SC for post-enrichment. All 4 combinations (SC, TT, SC-SC, and TT-SC) were used with the FA method to determine the best technique. Results were compared to the analysis with TT and SC by the AOAC culture method. In all studies, FA analysis with SC-SC gave the highest correlation with the AOAC method. In a total of 200 samples, the experienced group found 125 AOAC positives and 127 FA positives; no FA false-negatives and only 2 false-positives were reported. The inexperienced group reported 9 FA false-negatives and 5 FA false-positives. All false-negatives occurred in only 3 of the inexperienced laboratories. These studies showed that enrichment and post-enrichment in SC gave the best FA results and that training in FA methodology is required for correlation with existing AOAC methodology. The FA method for the detection of Salmonella has been adopted as official first action.", "contents": "Fluorescent antibody method for the detection of Salmonella: development, evaluation, and collaborative study. In preliminary studies, several commercial polyvalent fluorescent antibody (FA) preparations were evaluated for specificity and crossreactivity and an FA method was developed for the screening of Salmonella in products. Approximately 4000 product samples were tested by the FA method and the results were compared to those from the official final action AOAC method, 46.013-46.026. Only 4 FA false-negatives were found for a total of 619 confirmed positive Salmonella samples. The FA false-positive rate was 7%. The method was then subjected to a 2-phase collaborative study. In Phase I, 22 analysts tested 5 inoculated and 5 uninoculated samples of dried milk. In Phase II, 5 naturally contaminated and 5 presumably uncontaminated foods were analyzed. The study was designed to compare results from 11 analysts experienced in FA methodology with those from 11 analysts with little or no experience. Selenite cystine (SC) and tetrathionate (TT) broths were used for enrichment and both were inoculated into SC for post-enrichment. All 4 combinations (SC, TT, SC-SC, and TT-SC) were used with the FA method to determine the best technique. Results were compared to the analysis with TT and SC by the AOAC culture method. In all studies, FA analysis with SC-SC gave the highest correlation with the AOAC method. In a total of 200 samples, the experienced group found 125 AOAC positives and 127 FA positives; no FA false-negatives and only 2 false-positives were reported. The inexperienced group reported 9 FA false-negatives and 5 FA false-positives. All false-negatives occurred in only 3 of the inexperienced laboratories. These studies showed that enrichment and post-enrichment in SC gave the best FA results and that training in FA methodology is required for correlation with existing AOAC methodology. The FA method for the detection of Salmonella has been adopted as official first action."}
{"id": "PMID:1097389", "title": "Transport properties of merodiploids covering the dagA locus in Escherichia coli K-12.", "content": "A membrane componenet of the dag transport system which serves for glycine, D-alanine, and D-serine is coded for by the dagA gene at minute 83 of the Escherichia coli chromosome. Merodiploid strains (dagA+/dagA+) show two to three times the transport activity for only those amino acids that are substrates of the dag transport system. The increased transport activity is a result of a two-to threefold increase in Vmax for amino acid uptake with little or no change in the Km value. The two- to threefold gene dose effect of the merodiploid strains is maintained even during carbon starvation, eliminating the possibility that a greater energy supply for transport activity may account for the effect. Since merodiploids which carry more than one copy of the dagA allele show a gene dose response for transport activity, we conclude that the membrane componenet of the dag transport system which is coded for by the dagA allele is present in limiting amounts.", "contents": "Transport properties of merodiploids covering the dagA locus in Escherichia coli K-12. A membrane componenet of the dag transport system which serves for glycine, D-alanine, and D-serine is coded for by the dagA gene at minute 83 of the Escherichia coli chromosome. Merodiploid strains (dagA+/dagA+) show two to three times the transport activity for only those amino acids that are substrates of the dag transport system. The increased transport activity is a result of a two-to threefold increase in Vmax for amino acid uptake with little or no change in the Km value. The two- to threefold gene dose effect of the merodiploid strains is maintained even during carbon starvation, eliminating the possibility that a greater energy supply for transport activity may account for the effect. Since merodiploids which carry more than one copy of the dagA allele show a gene dose response for transport activity, we conclude that the membrane componenet of the dag transport system which is coded for by the dagA allele is present in limiting amounts."}
{"id": "PMID:1097390", "title": "Genetic characterization of the metK locus in Escherichia coli K-12.", "content": "Three independently isolated metK mutants have been shown to have leisions lying between speB and glc near 57 min on the Escherichia coli chromosome. Two deletions result in a lack of the metC gene product but neither extends into the metK glc region. The three metK mutations are recessive to the wild-type allele carried on the KLF16 episome.", "contents": "Genetic characterization of the metK locus in Escherichia coli K-12. Three independently isolated metK mutants have been shown to have leisions lying between speB and glc near 57 min on the Escherichia coli chromosome. Two deletions result in a lack of the metC gene product but neither extends into the metK glc region. The three metK mutations are recessive to the wild-type allele carried on the KLF16 episome."}
{"id": "PMID:1097391", "title": "New phosphoglucose isomerase mutants of Escherichia coli.", "content": "The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and the other a Mu-phage insertion, presumably both complete inactivation mutations. The new mutations do not give a phenotype markedly different from those described earlier. However, they might be preferred for certain physiological studies, and we have prepared for this a new double mutant, strain DF214, with a Mu insertion in pgi and a deletion in zwf (flucose 6-phosphate dehydrogenase).", "contents": "New phosphoglucose isomerase mutants of Escherichia coli. The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and the other a Mu-phage insertion, presumably both complete inactivation mutations. The new mutations do not give a phenotype markedly different from those described earlier. However, they might be preferred for certain physiological studies, and we have prepared for this a new double mutant, strain DF214, with a Mu insertion in pgi and a deletion in zwf (flucose 6-phosphate dehydrogenase)."}
{"id": "PMID:1097392", "title": "Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli.", "content": "We describe glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. The gene (gap) is at approximately 34 min, with the transductional order gap-fadD-eda. One gap mutant is temperature sensitive and has a heat-labile enzyme. Another is amber.", "contents": "Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. We describe glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. The gene (gap) is at approximately 34 min, with the transductional order gap-fadD-eda. One gap mutant is temperature sensitive and has a heat-labile enzyme. Another is amber."}
{"id": "PMID:1097393", "title": "Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase.", "content": "Genetic studies show that Escherichia coli has three enzymes capable of phosphorylating glucose: soluble adenosine 5'-triphosphate-dependent glucokinase, which plays only a minor role in glucose metabolism; an enzyme II, called glucosephosphotransferase, with high specificity for the D-glucose configuration; and another enzyme II, called mannosephosphotransferase, with broader specificity. The former enzyme II is active on glucose and methyl-alpha-glucopyranoside, whereas the latter is active on D-glucose, D-mannose, 2-deoxy-D-glucose, D-glucosamine, and D-mannosamine. Mutations leading to loss of glucosephosphotransferase activity and designated by the symbol gpt are between the purB and pyrC markers in a locus previously called cat. The locus of mutations to loss of mannosephosphotransferase, mpt, is between the eda and fadD genes. Mutations to loss of glucokinase, glk, are between the ptsI and dsd genes.", "contents": "Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. Genetic studies show that Escherichia coli has three enzymes capable of phosphorylating glucose: soluble adenosine 5'-triphosphate-dependent glucokinase, which plays only a minor role in glucose metabolism; an enzyme II, called glucosephosphotransferase, with high specificity for the D-glucose configuration; and another enzyme II, called mannosephosphotransferase, with broader specificity. The former enzyme II is active on glucose and methyl-alpha-glucopyranoside, whereas the latter is active on D-glucose, D-mannose, 2-deoxy-D-glucose, D-glucosamine, and D-mannosamine. Mutations leading to loss of glucosephosphotransferase activity and designated by the symbol gpt are between the purB and pyrC markers in a locus previously called cat. The locus of mutations to loss of mannosephosphotransferase, mpt, is between the eda and fadD genes. Mutations to loss of glucokinase, glk, are between the ptsI and dsd genes."}
{"id": "PMID:1097394", "title": "Specialized peptide transport system in Escherichia coli.", "content": "Trileucine is utilized as a source of leucine for growth of strains of Escherichia coli K-12 that are deficient in the oligopeptide transport system (Opp). Trithreonine is toxic to E. coli K-12. Opp- mutants of E. coli K-12 retain complete sensitivity to this tripeptide. Moreover, E. coli W, which is resistant to trithreonine, can utlize this tripeptide as a threonine source and this capability is fully maintained in E. coli W (Opp-). A spontaneous trithreonine-resistant mutant of E. coli K-12 (Opp-) has been isolated that has an impaired growth response to trileucine and is resistant to trithreonine. Trileucine competes with the uptake of trithreonine as measured by its ability to relieve trithreonine toxicity in E. coli K-12. It is concluded that trileucine as well as trithreonine are transported into E. coli K-12 or W by a common uptake system that is distinct from the Opp system. Trimethionine can act as a competitor of trileucine or trithreonine-supported growth and as an antagonist of trithreonine toxicity in Opp- mutants. It is concluded that trimethionine is recognized by the trileucine-trithreonine transport system. Trithreonine, trimethionine, and trileucine are also transported by the Opp system, as they all relieve triornithine toxicity towards E. coli W and compete with tetralysine utilization as lysine source for growth of a lysine auxotroph of this strain.", "contents": "Specialized peptide transport system in Escherichia coli. Trileucine is utilized as a source of leucine for growth of strains of Escherichia coli K-12 that are deficient in the oligopeptide transport system (Opp). Trithreonine is toxic to E. coli K-12. Opp- mutants of E. coli K-12 retain complete sensitivity to this tripeptide. Moreover, E. coli W, which is resistant to trithreonine, can utlize this tripeptide as a threonine source and this capability is fully maintained in E. coli W (Opp-). A spontaneous trithreonine-resistant mutant of E. coli K-12 (Opp-) has been isolated that has an impaired growth response to trileucine and is resistant to trithreonine. Trileucine competes with the uptake of trithreonine as measured by its ability to relieve trithreonine toxicity in E. coli K-12. It is concluded that trileucine as well as trithreonine are transported into E. coli K-12 or W by a common uptake system that is distinct from the Opp system. Trimethionine can act as a competitor of trileucine or trithreonine-supported growth and as an antagonist of trithreonine toxicity in Opp- mutants. It is concluded that trimethionine is recognized by the trileucine-trithreonine transport system. Trithreonine, trimethionine, and trileucine are also transported by the Opp system, as they all relieve triornithine toxicity towards E. coli W and compete with tetralysine utilization as lysine source for growth of a lysine auxotroph of this strain."}
{"id": "PMID:1097395", "title": "Multiplicity of oligopeptide transport systems in Escherichia coli.", "content": "The ability of Escherichia coli K-12 4212 to utilize a variety of oligopeptides as sources of required amino acids was examined. Triornithine-resistant mutants of this strain were oligopeptide permease deficient (Opp-) as judged by their inability to utilize (Lys)3 and (Lys)4 as sources of lysine and their resistance to the toxic tripeptide (Val)3. These same mutants were able to grow when Met-Met-Met, Met-Gly-Met, Met-Gly-Gly, Gly-Met-Gly, Gly-Gly-Met, Gly-Met-Met, Met-Met-Gly, or Leu-Leu-Leu were supplied in place of the requisite amino acid. The system mediating the uptake of these peptides, herein designated Opr I, was not able to transport N-alpha-acetylated peptides, nor the tetrapeptides Met-Gly-Met-Met, Met-Met-Gly-Met, or Met-Met-Met-Gly. Competition experiments indicated that trimethionine and trileucine enter E. coli K-12 via either Opp or Opr I. Analogous results were found using the methionine, leucine-requiring auxotroph E. coli B163. It appears that more than one oligopeptide transport system exists in E. coli and that the system mediating peptide uptake is complex.", "contents": "Multiplicity of oligopeptide transport systems in Escherichia coli. The ability of Escherichia coli K-12 4212 to utilize a variety of oligopeptides as sources of required amino acids was examined. Triornithine-resistant mutants of this strain were oligopeptide permease deficient (Opp-) as judged by their inability to utilize (Lys)3 and (Lys)4 as sources of lysine and their resistance to the toxic tripeptide (Val)3. These same mutants were able to grow when Met-Met-Met, Met-Gly-Met, Met-Gly-Gly, Gly-Met-Gly, Gly-Gly-Met, Gly-Met-Met, Met-Met-Gly, or Leu-Leu-Leu were supplied in place of the requisite amino acid. The system mediating the uptake of these peptides, herein designated Opr I, was not able to transport N-alpha-acetylated peptides, nor the tetrapeptides Met-Gly-Met-Met, Met-Met-Gly-Met, or Met-Met-Met-Gly. Competition experiments indicated that trimethionine and trileucine enter E. coli K-12 via either Opp or Opr I. Analogous results were found using the methionine, leucine-requiring auxotroph E. coli B163. It appears that more than one oligopeptide transport system exists in E. coli and that the system mediating peptide uptake is complex."}
{"id": "PMID:1097396", "title": "chlD gene function in molybdate activation of nitrate reductase.", "content": "chlD mutants of Escherichia coli lack active nitrate reductase but form normal levels of this enzyme when the medium is supplemented with 10-3 M molybdate. When chlD mutants were grown in unsupplemented medium and then incubated with molybdate in the presence of chloramphenicol, they formed about 5% the normal level of nitrate reductase. Some chlD mutants or the wild type grown in medium supplemented with tungstate accumulated an inactive protein which was electrophoretically identical to active nitrate reductase. Addition of molybdate to those cells in the presence of chloramphenicol resulted in the formation of fully induced levels of nitrate reductase. Two chlD mutants, including a deletion mutant, failed to accumulate the inactive protein and to form active enzyme under the same conditions. Insertion of 99-Mo into the enzyme protein paralleled activation; 185-W could not be demonstrated to be associated with the accumulated inactive protein. The rates of activation of nitrate reductase at varying molybdate concentrations indicated that the chlD gene product facilitates the activation of nitrate reductase at concentrations of molybdate found in normal growth media. At high concentrations, molybdate circumvented this function in chlD mutants and appeared to activate nitrate reductase by a mass action process. We conclude that the chlD gene plays two distinguishable roles in the formation of nitrate reductase in E. coli. It is involved in the accumulation of fully induced levels of the nitrate reductase protein in the cell membrane and it facilitates the insertion of molybdenum to form the active enzyme.", "contents": "chlD gene function in molybdate activation of nitrate reductase. chlD mutants of Escherichia coli lack active nitrate reductase but form normal levels of this enzyme when the medium is supplemented with 10-3 M molybdate. When chlD mutants were grown in unsupplemented medium and then incubated with molybdate in the presence of chloramphenicol, they formed about 5% the normal level of nitrate reductase. Some chlD mutants or the wild type grown in medium supplemented with tungstate accumulated an inactive protein which was electrophoretically identical to active nitrate reductase. Addition of molybdate to those cells in the presence of chloramphenicol resulted in the formation of fully induced levels of nitrate reductase. Two chlD mutants, including a deletion mutant, failed to accumulate the inactive protein and to form active enzyme under the same conditions. Insertion of 99-Mo into the enzyme protein paralleled activation; 185-W could not be demonstrated to be associated with the accumulated inactive protein. The rates of activation of nitrate reductase at varying molybdate concentrations indicated that the chlD gene product facilitates the activation of nitrate reductase at concentrations of molybdate found in normal growth media. At high concentrations, molybdate circumvented this function in chlD mutants and appeared to activate nitrate reductase by a mass action process. We conclude that the chlD gene plays two distinguishable roles in the formation of nitrate reductase in E. coli. It is involved in the accumulation of fully induced levels of the nitrate reductase protein in the cell membrane and it facilitates the insertion of molybdenum to form the active enzyme."}
{"id": "PMID:1097397", "title": "Genetic and physical studies of lambda transducing bacteriophage carrying the beta subunit gene of the Escherichia coli ribonucleic acid polymerase.", "content": "The prophage lambdac1857 was inserted into the bfe gene located near rif (the structural gene for the beta subunit of deoxyribonucleic acid [DNA]-dependent ribonucleic acid polymerase) on the Escherichia coli chromosome. Induced lysates (low-frequency transducing lysates) of such a lysogen contained defective lambda phage particles (lambdadrif+) that can specifically transduce the wild-type rif+ gene. Upon transduction into a recipient strain carrying recA, heterogenotes harboring both the wild-type and the mutant rif genes were isolated. Rec+ derivatives of these heterogenotes produce high-frequency transducing lysates that contain lambdadrif+ and normal active phages at a ratio of 1 to 2. The results of marker rescue experiments and of density determination with several transducing phages indicate that most of the late genes are deleted and replaced by a segment of the chromosomal DNA carrying the bfe-rif region. The length of the chromosomal segment seems to vary between approximately 0.5 and 0.6% of the total bacterial DNA among the three independently isolated lambdadrif+ phages. Electron microscopy of heteroduplex DNA consisting of one strand from lambdadrif+-6 and the other from lambdaimm-21 phages directly confirmed that most of the phage DNA of the \"left arm\" was replaced by the bacterial DNA. The heteroduplex study also demonstrated that the integration of prophage lambda into the bfe region occurred at the normal cross-over point within the phage attachment site.", "contents": "Genetic and physical studies of lambda transducing bacteriophage carrying the beta subunit gene of the Escherichia coli ribonucleic acid polymerase. The prophage lambdac1857 was inserted into the bfe gene located near rif (the structural gene for the beta subunit of deoxyribonucleic acid [DNA]-dependent ribonucleic acid polymerase) on the Escherichia coli chromosome. Induced lysates (low-frequency transducing lysates) of such a lysogen contained defective lambda phage particles (lambdadrif+) that can specifically transduce the wild-type rif+ gene. Upon transduction into a recipient strain carrying recA, heterogenotes harboring both the wild-type and the mutant rif genes were isolated. Rec+ derivatives of these heterogenotes produce high-frequency transducing lysates that contain lambdadrif+ and normal active phages at a ratio of 1 to 2. The results of marker rescue experiments and of density determination with several transducing phages indicate that most of the late genes are deleted and replaced by a segment of the chromosomal DNA carrying the bfe-rif region. The length of the chromosomal segment seems to vary between approximately 0.5 and 0.6% of the total bacterial DNA among the three independently isolated lambdadrif+ phages. Electron microscopy of heteroduplex DNA consisting of one strand from lambdadrif+-6 and the other from lambdaimm-21 phages directly confirmed that most of the phage DNA of the \"left arm\" was replaced by the bacterial DNA. The heteroduplex study also demonstrated that the integration of prophage lambda into the bfe region occurred at the normal cross-over point within the phage attachment site."}
{"id": "PMID:1097398", "title": "D-Alanine-requiring cell wall mutant of Escherichia coli.", "content": "A mutant of Escherichia coli is described whose cells show a spherical or irregular morphology, associated with leakage of beta-galactosidase and other intracellular proteins. The expression of the morphologic abnormality is most marked when the mutant is grown in rich media and is suppressed by D-alamine, D-serine, D-glutamate, or glycine supplementation. D-Alanine is the most effective amino acid supplement, half maximally supressing this anomalous property at a concentration of 75 mug/ml, as measured by the reduction in beta-galactosidase released from the cells. The mutant is more sensitive to penicillin G, D-methionine, and D-valine and it is relatively resistant to lysozyme. These phenotypic abnormalities are likewise corrected by the above supplementations. The relative rates of peptidoglycan synthesis in mutant and parent, grown under restrictive conditions, were measured both in vivo and in vitro by rates of incorporation of L-[14-D]alanine and uridine-5'-diphosphate-N-acetyl-D-[1-15C-A1-glucosamine, respectively. There is not metabolic block in the biosynthesis of uridine-5'-diphosphate-N-acetyl-muramyl-pentapeptide as shown by enzymic analysis and the lack of accumulation of uridine-5'-diphosphate-N-acetylmuramyl-peptide precursors. These preliminary studies suggest that the mutant possesses a defect in the biosynthesis of peptidoglycan although the exact lesion has not yet been established.", "contents": "D-Alanine-requiring cell wall mutant of Escherichia coli. A mutant of Escherichia coli is described whose cells show a spherical or irregular morphology, associated with leakage of beta-galactosidase and other intracellular proteins. The expression of the morphologic abnormality is most marked when the mutant is grown in rich media and is suppressed by D-alamine, D-serine, D-glutamate, or glycine supplementation. D-Alanine is the most effective amino acid supplement, half maximally supressing this anomalous property at a concentration of 75 mug/ml, as measured by the reduction in beta-galactosidase released from the cells. The mutant is more sensitive to penicillin G, D-methionine, and D-valine and it is relatively resistant to lysozyme. These phenotypic abnormalities are likewise corrected by the above supplementations. The relative rates of peptidoglycan synthesis in mutant and parent, grown under restrictive conditions, were measured both in vivo and in vitro by rates of incorporation of L-[14-D]alanine and uridine-5'-diphosphate-N-acetyl-D-[1-15C-A1-glucosamine, respectively. There is not metabolic block in the biosynthesis of uridine-5'-diphosphate-N-acetyl-muramyl-pentapeptide as shown by enzymic analysis and the lack of accumulation of uridine-5'-diphosphate-N-acetylmuramyl-peptide precursors. These preliminary studies suggest that the mutant possesses a defect in the biosynthesis of peptidoglycan although the exact lesion has not yet been established."}
{"id": "PMID:1097399", "title": "Ultrastructural studies of sporulation in Bacillus sphaericus.", "content": "Spore septum formation in Bacillus sphaericus 9602 occurs 2 h after the end of exponential growth at one end of the vegetative cell, which retains a uniform diameter. The apparently rigid spore septum contains an inner cell wall layer which disappears when the sporulation septum \"bulges\" into the mother cell cytoplasm. This process occurs simultaneously with terminal swelling at the end of the cell containing the spore septum. It is suggested that the inner cell wall layer is peptidoglycan and that its dissolution and the terminal swelling are consequences of a localized autolysis. Engulfment of the forespore by membrane proliferation results in the production of a forespore surrounded by two flexible, closely apposed membranes. These membranes appear to become more rigid as a peptidoglycan-like layer appears between them, concomitant with the condensation of the forespore nucleoid into a crescent-shaped structure. After nuclear condensation, visible development of distinct cortex, primordial cell wall, and spore coat layers begin, and the forespore cytoplasm assumes an appearance similar to that of a refractile spore. The spore coats consist of an amorphous inner layer, a lamellar midlayer, and a structured outer layer. As cortex synthesis and spore coat assembly continue, exosporium development commences close to that portion of the mother cell plasma membrane which surrounds the forespore. The exosporium is lamellar and in tangential section is seen to have a hexagonal arrangement of subunits. The timing of these morphological events has the expected correlation with the appearance of unique enzyme activites required for cortex synthesis.", "contents": "Ultrastructural studies of sporulation in Bacillus sphaericus. Spore septum formation in Bacillus sphaericus 9602 occurs 2 h after the end of exponential growth at one end of the vegetative cell, which retains a uniform diameter. The apparently rigid spore septum contains an inner cell wall layer which disappears when the sporulation septum \"bulges\" into the mother cell cytoplasm. This process occurs simultaneously with terminal swelling at the end of the cell containing the spore septum. It is suggested that the inner cell wall layer is peptidoglycan and that its dissolution and the terminal swelling are consequences of a localized autolysis. Engulfment of the forespore by membrane proliferation results in the production of a forespore surrounded by two flexible, closely apposed membranes. These membranes appear to become more rigid as a peptidoglycan-like layer appears between them, concomitant with the condensation of the forespore nucleoid into a crescent-shaped structure. After nuclear condensation, visible development of distinct cortex, primordial cell wall, and spore coat layers begin, and the forespore cytoplasm assumes an appearance similar to that of a refractile spore. The spore coats consist of an amorphous inner layer, a lamellar midlayer, and a structured outer layer. As cortex synthesis and spore coat assembly continue, exosporium development commences close to that portion of the mother cell plasma membrane which surrounds the forespore. The exosporium is lamellar and in tangential section is seen to have a hexagonal arrangement of subunits. The timing of these morphological events has the expected correlation with the appearance of unique enzyme activites required for cortex synthesis."}
{"id": "PMID:1097400", "title": "Derivation of glycine from threonine in Escherichia coli K-12 mutants.", "content": "Escherichia coli AT2046 has been shown previously to lack the enzyme serine transhydroxymethylase and to require exogenous glycine for growth as a consequence. Strains JEV73 and JEV73R, mutants derived from strain AT2046, are shown here to be serine transhydroxymethylase deficient, but able to derive their glycine from endogenously synthesized threonine. Leucine is shown to be closely involved in the regulation of biosynthesis of glycine, to spare glycine in strain AT2046T, to replace glycine in strain JEV73, and to increase threonine conversion to glycine in a representative prototroph of E. coli. An interpretation of strains JEV73 and JEV73R as regulatory mutants of strain AT2046 is given. A hypothesis as to the role of leucine as a signal for nitrogen scavenging is suggested.", "contents": "Derivation of glycine from threonine in Escherichia coli K-12 mutants. Escherichia coli AT2046 has been shown previously to lack the enzyme serine transhydroxymethylase and to require exogenous glycine for growth as a consequence. Strains JEV73 and JEV73R, mutants derived from strain AT2046, are shown here to be serine transhydroxymethylase deficient, but able to derive their glycine from endogenously synthesized threonine. Leucine is shown to be closely involved in the regulation of biosynthesis of glycine, to spare glycine in strain AT2046T, to replace glycine in strain JEV73, and to increase threonine conversion to glycine in a representative prototroph of E. coli. An interpretation of strains JEV73 and JEV73R as regulatory mutants of strain AT2046 is given. A hypothesis as to the role of leucine as a signal for nitrogen scavenging is suggested."}
{"id": "PMID:1097401", "title": "Rate stimulation of deoxyribonucleic acid synthesis after inhibition.", "content": "The degree to which the rate of deoxyribonucleic acid synthesis in thy- cultures of Escherichia coli is stimulated after a period of thymine starvation is shown to be a function of the concentration of thymine present as well as of the culture doubling time. Inhibition of deoxyribonucleic acid synthesis by nalidixic acid yields comparable results. Periods of thymine starvation exceeding one doubling time appear to cause an irreversible inactivation of a fraction of the replication forks in the culture.", "contents": "Rate stimulation of deoxyribonucleic acid synthesis after inhibition. The degree to which the rate of deoxyribonucleic acid synthesis in thy- cultures of Escherichia coli is stimulated after a period of thymine starvation is shown to be a function of the concentration of thymine present as well as of the culture doubling time. Inhibition of deoxyribonucleic acid synthesis by nalidixic acid yields comparable results. Periods of thymine starvation exceeding one doubling time appear to cause an irreversible inactivation of a fraction of the replication forks in the culture."}
{"id": "PMID:1097402", "title": "Dominant and semidominant mutations leading to thermosensitivity of ribonucleic acid biosynthesis in Saccharomyces cerevisiae.", "content": "Different dominant thermosensitive mutations affecting the same gene were selected in Saccharomyces cerevisiae. Ribonucleic acid (RNA) synthesis decreased rapidly and markedly at 37 C in all the mutants whether they were in a homozygous or a heterozygous state. Protein biosynthesis was at first unaffected and then decreased slowly, stopping after 5 h. Measurements of RNA biosynthesis in isolated nuclei as well as in vitro activities of RNA polymerases A and B at 22 and 37 C failed to reveal any difference between mutants and the wild type. Analysis of the nature of the residual RNAs synthesized at the high temperature in the mutants showed a small relative increase in the messenger RNA fraction, but it was not sufficient to indicate a specific inactivation of RNA polymerase A activity. The results suggest an impairment in a common regulatory element for all RNA polymerases acting at the level of the initiation of transcription. Similar mutants with a semidominant phenotype were obtained in which the lesions were in two other unlinked loci.", "contents": "Dominant and semidominant mutations leading to thermosensitivity of ribonucleic acid biosynthesis in Saccharomyces cerevisiae. Different dominant thermosensitive mutations affecting the same gene were selected in Saccharomyces cerevisiae. Ribonucleic acid (RNA) synthesis decreased rapidly and markedly at 37 C in all the mutants whether they were in a homozygous or a heterozygous state. Protein biosynthesis was at first unaffected and then decreased slowly, stopping after 5 h. Measurements of RNA biosynthesis in isolated nuclei as well as in vitro activities of RNA polymerases A and B at 22 and 37 C failed to reveal any difference between mutants and the wild type. Analysis of the nature of the residual RNAs synthesized at the high temperature in the mutants showed a small relative increase in the messenger RNA fraction, but it was not sufficient to indicate a specific inactivation of RNA polymerase A activity. The results suggest an impairment in a common regulatory element for all RNA polymerases acting at the level of the initiation of transcription. Similar mutants with a semidominant phenotype were obtained in which the lesions were in two other unlinked loci."}
{"id": "PMID:1097403", "title": "Effect of growth rate on the amounts of ribosomal and transfer ribonucleic acids in yeast.", "content": "The steady-state growth rate of Saccharomyces cerevisiae was varied by growing the cells in different media. The total amount of ribonucleic acid (RNA) per cell was found to decrease as a nonlinear function of decreasing growh rate. The RNA from cells growing in different media was analyzed by polyacrylamide gel electrophoresis. Although the amounts of both ribosomal RNA and transfer RNA decreased with decreasing growth rate, the ratio of ribosomal to transfer RNA was not constant. As the growth rate was reduced the ribosomal RNA fraction decreased slightly, whereas the transfer RNA fraction increased slightly. Thus the levels of ribosomal and transfer RNA were regulated to similar yet different extents. The levels of the different ribosomal RNA species were more closely coordinated. At all growth rates the ribosomal RNAs (including 5S RNA) were present in equimolar amounts. The rate of protein synthesis in yeast cells also decreased with decreasing growth rate. The low rates of protein synthesis did not appear to be due to limiting numbers of ribosomes or transfer RNA molecules.", "contents": "Effect of growth rate on the amounts of ribosomal and transfer ribonucleic acids in yeast. The steady-state growth rate of Saccharomyces cerevisiae was varied by growing the cells in different media. The total amount of ribonucleic acid (RNA) per cell was found to decrease as a nonlinear function of decreasing growh rate. The RNA from cells growing in different media was analyzed by polyacrylamide gel electrophoresis. Although the amounts of both ribosomal RNA and transfer RNA decreased with decreasing growth rate, the ratio of ribosomal to transfer RNA was not constant. As the growth rate was reduced the ribosomal RNA fraction decreased slightly, whereas the transfer RNA fraction increased slightly. Thus the levels of ribosomal and transfer RNA were regulated to similar yet different extents. The levels of the different ribosomal RNA species were more closely coordinated. At all growth rates the ribosomal RNAs (including 5S RNA) were present in equimolar amounts. The rate of protein synthesis in yeast cells also decreased with decreasing growth rate. The low rates of protein synthesis did not appear to be due to limiting numbers of ribosomes or transfer RNA molecules."}
{"id": "PMID:1097404", "title": "Interaction of the expression of two membrane genes, acrA and plsA, in Escherichia coli K-12.", "content": "The mutation acrA1, leading to acriflavine sensitivity through disorganization of the plasma membrane, is located between proC and purE on the Escherichia coli K-12 chromosome. Gene plsA has been reported to determine biosynthesis of membrane phospholipid and to be located very near acrA (1). Genes acrA and plsA fall into different cistrons and are arranged in the order proC-acrA-plasA-purE. The genes were shown to interact with each other. Introduction of acrA mutation into a plsA temperature-sensitive mutant mitigated the heat sensitivity. Plasmid (F-gal+) stability in acrA mutants was restored by introduction of the plasA mutation into the acrA cells. When an Hfr plsA donor was conjugated with an acrA recipient, or when reciprocally conjugated, the exogenotes were eliminated at high frequency during subsequent subcultivation in broth. However, the exogenotes were not eliminated in all other allelic combinations of genes acrA and plsA. When an F-gal+ plasmid was introduced into the unstable heterozygotes (acrA+plsA/acrApls1+), the plasmids were stably hosted, whereas the acrA+ plasA exogenotes were spontaneously lost at a high frequency. On the other hand, when the unstable heterozygotes carrying F-gal+ were cultured in acriflavine-containing medium, the F-gal+ plasmids were preferentially eliminated but the acrA+plasA exogenotes were not affected. The results suggest that the organization of the plasma membrane controls the recombination of the exogenotes introduced into zygotes.", "contents": "Interaction of the expression of two membrane genes, acrA and plsA, in Escherichia coli K-12. The mutation acrA1, leading to acriflavine sensitivity through disorganization of the plasma membrane, is located between proC and purE on the Escherichia coli K-12 chromosome. Gene plsA has been reported to determine biosynthesis of membrane phospholipid and to be located very near acrA (1). Genes acrA and plsA fall into different cistrons and are arranged in the order proC-acrA-plasA-purE. The genes were shown to interact with each other. Introduction of acrA mutation into a plsA temperature-sensitive mutant mitigated the heat sensitivity. Plasmid (F-gal+) stability in acrA mutants was restored by introduction of the plasA mutation into the acrA cells. When an Hfr plsA donor was conjugated with an acrA recipient, or when reciprocally conjugated, the exogenotes were eliminated at high frequency during subsequent subcultivation in broth. However, the exogenotes were not eliminated in all other allelic combinations of genes acrA and plsA. When an F-gal+ plasmid was introduced into the unstable heterozygotes (acrA+plsA/acrApls1+), the plasmids were stably hosted, whereas the acrA+ plasA exogenotes were spontaneously lost at a high frequency. On the other hand, when the unstable heterozygotes carrying F-gal+ were cultured in acriflavine-containing medium, the F-gal+ plasmids were preferentially eliminated but the acrA+plasA exogenotes were not affected. The results suggest that the organization of the plasma membrane controls the recombination of the exogenotes introduced into zygotes."}
{"id": "PMID:1097405", "title": "Partial suppression of the phenotype of Escherichia coli K-12 dnaG mutants by some I-like conjugative plasmids.", "content": "Three I-like conjugative plasmids, ColIdrd1, R144drd3, and R64drd11, which are derepressed for functions involved in conjugation, were found to suppress at least partially the phenotype of temperature-sensitive dnaG mutants of Escherichia coli K-12, as judged from the kinetics of deoxyribonucleic acid synthesis at elevated temperature in newly formed and established plasmid-containing strains. In contrast, the corresponding wild-type plasmids and three F-like derepressed conjugative plasmids, F101, R100drd1, and R1drd16, all failed to suppress. Suppression is presumably caused by a different plasmid-determined function from that which promotes survival of ultraviolet-irradiated bacteria, because both the wild-type I-like plasmids and their drd mutants protected irradiated bacteria. One possible interpretation of these results is that the product of a gene carried by certain I-like plasmids can substitute for the bacterial dnaG gene product during ongoing deoxyribonucleic acid replication.", "contents": "Partial suppression of the phenotype of Escherichia coli K-12 dnaG mutants by some I-like conjugative plasmids. Three I-like conjugative plasmids, ColIdrd1, R144drd3, and R64drd11, which are derepressed for functions involved in conjugation, were found to suppress at least partially the phenotype of temperature-sensitive dnaG mutants of Escherichia coli K-12, as judged from the kinetics of deoxyribonucleic acid synthesis at elevated temperature in newly formed and established plasmid-containing strains. In contrast, the corresponding wild-type plasmids and three F-like derepressed conjugative plasmids, F101, R100drd1, and R1drd16, all failed to suppress. Suppression is presumably caused by a different plasmid-determined function from that which promotes survival of ultraviolet-irradiated bacteria, because both the wild-type I-like plasmids and their drd mutants protected irradiated bacteria. One possible interpretation of these results is that the product of a gene carried by certain I-like plasmids can substitute for the bacterial dnaG gene product during ongoing deoxyribonucleic acid replication."}
{"id": "PMID:1097406", "title": "Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae.", "content": "Two new classes of mutants containing recessive constitutive mutations, phoT and phoU, that affect the repressible acid phosphatase (EC 3.1.3.2) in Saccharomyces cerevisiae were isolated along with many previously known phoR mutants. These loci segregated independently from each other, from the phoS gene, and from another regulatory gene, phoD, that exerts positive control for acid phosphatase synthesis. The phoR and phoU mutations showed the same genetic behavior in the double mutants, which also contained the phoS or phoD mutation. In contrast, the phoT mutation could not suppress the phoS mutation, which caused a loss of enzyme activity. Many mutant alleles of phoR and phoU were found to be temperature sensitive (ts), whereas those of phoT were not. These ts mutants were constitutive at 35 C but severely repressible at 25 C. These facts strongly suggest that both the phoR and phoU genes are cooperatively concerned with the production of the repressor, whereas the phoT gene might be involved in another mechanism distinct from that in which phoR and phoU are involved. No single mutation of phoR, phoT, or phoU result in an enzyme level comparable to that of fully derepressed enzyme activities, and the temperature sensitivity of the ts phoR and ts phoU mutations in such combinations almost disappeared. In addition to these observations, since the ts phoR phoS and ts phoU phoS double mutants showed some enzyme synthesis at 25 C under derepressing conditions, a defect in the ts mutant repressors was strongly suggested, even at 25 C.", "contents": "Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae. Two new classes of mutants containing recessive constitutive mutations, phoT and phoU, that affect the repressible acid phosphatase (EC 3.1.3.2) in Saccharomyces cerevisiae were isolated along with many previously known phoR mutants. These loci segregated independently from each other, from the phoS gene, and from another regulatory gene, phoD, that exerts positive control for acid phosphatase synthesis. The phoR and phoU mutations showed the same genetic behavior in the double mutants, which also contained the phoS or phoD mutation. In contrast, the phoT mutation could not suppress the phoS mutation, which caused a loss of enzyme activity. Many mutant alleles of phoR and phoU were found to be temperature sensitive (ts), whereas those of phoT were not. These ts mutants were constitutive at 35 C but severely repressible at 25 C. These facts strongly suggest that both the phoR and phoU genes are cooperatively concerned with the production of the repressor, whereas the phoT gene might be involved in another mechanism distinct from that in which phoR and phoU are involved. No single mutation of phoR, phoT, or phoU result in an enzyme level comparable to that of fully derepressed enzyme activities, and the temperature sensitivity of the ts phoR and ts phoU mutations in such combinations almost disappeared. In addition to these observations, since the ts phoR phoS and ts phoU phoS double mutants showed some enzyme synthesis at 25 C under derepressing conditions, a defect in the ts mutant repressors was strongly suggested, even at 25 C."}
{"id": "PMID:1097407", "title": "Relationship between chromosome replication and cell division in a thymineless mutant of Escherichia coli B/r.", "content": "The relationship between chromosome replication and cell division was investigated in a thymineless mutant of Escherichia coli B/r. Examination of the changes in average cell mass and DNA content of exponential cultures resulting from changes in the thymine concentration in the growth medium suggested that as the replication time (C) is increased there is a decrease in the period between termination of a round of replication and the subsequent cell division (D). Observations on the pattern of DNA synthesis during the division cycle were consistent with this relationship. Nevertheless, the kinetics of transition of exponential cultures moving between steady states of growth with differing replication velocities provided evidence to support the view that the time of cell division is determined by termination of rounds of replication under steady-state conditions.", "contents": "Relationship between chromosome replication and cell division in a thymineless mutant of Escherichia coli B/r. The relationship between chromosome replication and cell division was investigated in a thymineless mutant of Escherichia coli B/r. Examination of the changes in average cell mass and DNA content of exponential cultures resulting from changes in the thymine concentration in the growth medium suggested that as the replication time (C) is increased there is a decrease in the period between termination of a round of replication and the subsequent cell division (D). Observations on the pattern of DNA synthesis during the division cycle were consistent with this relationship. Nevertheless, the kinetics of transition of exponential cultures moving between steady states of growth with differing replication velocities provided evidence to support the view that the time of cell division is determined by termination of rounds of replication under steady-state conditions."}
{"id": "PMID:1097408", "title": "Utilization of selected leucine peptide amides by Escherichia coli.", "content": "Studies on the utilization of leucine peptide amides as a source of leucine for a leucine auxotroph showed that in general compounds with the structure leu-chi amide (where chi is any amide) are utilized as well as the free peptide, but that compounds with the structure chi-leu amide (where chi is not leucine) are used less effectively than the free peptide. Growth and enzymological experiments indicated that the lower capacity of Escherichia coli to utilize amides of the structure chi-leu amide is not a result of poor transport of these compounds, but rather the inability to rapidly liberate leucine from the amide when it is supplied to the cell in the form of a peptide. Competition studies indicated that the peptide amides enter the cell via the oligopeptide permease system.", "contents": "Utilization of selected leucine peptide amides by Escherichia coli. Studies on the utilization of leucine peptide amides as a source of leucine for a leucine auxotroph showed that in general compounds with the structure leu-chi amide (where chi is any amide) are utilized as well as the free peptide, but that compounds with the structure chi-leu amide (where chi is not leucine) are used less effectively than the free peptide. Growth and enzymological experiments indicated that the lower capacity of Escherichia coli to utilize amides of the structure chi-leu amide is not a result of poor transport of these compounds, but rather the inability to rapidly liberate leucine from the amide when it is supplied to the cell in the form of a peptide. Competition studies indicated that the peptide amides enter the cell via the oligopeptide permease system."}
{"id": "PMID:1097409", "title": "Separate regulation of transport and biosynthesis of leucine, isoleucine, and valine in bacteria.", "content": "Since both transport activity and the leucine biosynthetic enzymes are repressed by growth on leucine, the regulation of leucine, isoleucine, and valine biosynthetic enzymes was examined in Escherichia coli K-12 strain EO312, a constitutively derepressed branched-chain amino acid transport mutant, to determine if the transport derepression affected the biosynthetic enzymes. Neither the iluB gene product, acetohydroxy acid synthetase (acetolactate synthetase, EC 4.1.3.18), NOR THE LEUB gene product, 3-isopropylmalate dehydrogenase (2-hydroxy-4-methyl-3-carboxyvalerate-nicotinamide adenine dinucleotide oxido-reductase, EC 1.1.1.85), were significantly affected in their level of derepression or repression compared to the parental strain. A number of strains with alterations in the regulation of the branched-chain amino acid biosynthetic enzymes were examined for the regulation of the shock-sensitive transport system for these amino acids (LIV-I). When transport activity was examined in strains with mutations leading to derepression of the iluB, iluADE, and leuABCD gene clusters, the regulation of the LIV-I transport system was found to be normal. The regulation of transport in an E. coli strain B/r with a deletion of the entire leucine biosynthetic operon was normal, indicating none of the gene products of this operon are required for regulation of transport. Salmonella typhimurium LT2 strain leu-500, a single-site mutation affecting both promotor-like and operator-like function of the leuABCD gene cluster, also had normal regulation of the LIV-I transport system. All of the strains contained leucine-specific transport activity, which was also repressed by growth in media containing leucine, isoleucine and valine. The concentrated shock fluids from these strains grown in minimal medium or with excess leucine, isoleucine, and valine were examined for proteins with leucine-binding activity, and the levels of these proteins were found to be regulated normally. It appears that the branched-chain amino acid transport systems and biosynthetic enzymes in E. coli strains K-12 and B/r and in S. typhimurium strain LT2 are not regulated together by a cis-dominate type of mechanism, although both systems may have components in common.", "contents": "Separate regulation of transport and biosynthesis of leucine, isoleucine, and valine in bacteria. Since both transport activity and the leucine biosynthetic enzymes are repressed by growth on leucine, the regulation of leucine, isoleucine, and valine biosynthetic enzymes was examined in Escherichia coli K-12 strain EO312, a constitutively derepressed branched-chain amino acid transport mutant, to determine if the transport derepression affected the biosynthetic enzymes. Neither the iluB gene product, acetohydroxy acid synthetase (acetolactate synthetase, EC 4.1.3.18), NOR THE LEUB gene product, 3-isopropylmalate dehydrogenase (2-hydroxy-4-methyl-3-carboxyvalerate-nicotinamide adenine dinucleotide oxido-reductase, EC 1.1.1.85), were significantly affected in their level of derepression or repression compared to the parental strain. A number of strains with alterations in the regulation of the branched-chain amino acid biosynthetic enzymes were examined for the regulation of the shock-sensitive transport system for these amino acids (LIV-I). When transport activity was examined in strains with mutations leading to derepression of the iluB, iluADE, and leuABCD gene clusters, the regulation of the LIV-I transport system was found to be normal. The regulation of transport in an E. coli strain B/r with a deletion of the entire leucine biosynthetic operon was normal, indicating none of the gene products of this operon are required for regulation of transport. Salmonella typhimurium LT2 strain leu-500, a single-site mutation affecting both promotor-like and operator-like function of the leuABCD gene cluster, also had normal regulation of the LIV-I transport system. All of the strains contained leucine-specific transport activity, which was also repressed by growth in media containing leucine, isoleucine and valine. The concentrated shock fluids from these strains grown in minimal medium or with excess leucine, isoleucine, and valine were examined for proteins with leucine-binding activity, and the levels of these proteins were found to be regulated normally. It appears that the branched-chain amino acid transport systems and biosynthetic enzymes in E. coli strains K-12 and B/r and in S. typhimurium strain LT2 are not regulated together by a cis-dominate type of mechanism, although both systems may have components in common."}
{"id": "PMID:1097410", "title": "Random replication of the stringent plasmid R1 in Escherichia coli K-12.", "content": "The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The results are in quantitative accordance with a random replication of R1 in which the replicating molecules are taken from a cytoplasmic plasmid pool and transferred back to the pool after replication. This is analogous to the results obtained by Bazaral and Helinski (1970) and Rownd (1969) for plasmids that are present in 10 to 20 copies per genome (so-called relaxed control of replication). Hence, there seem to be no difference between stringent and relaxed plasmids with respect to selection of plasmid molecules for replication. However, we cannot tell whether all R1 copies in a cell replicate during a fraction of or throughout the cell cycle. The random selction of plasmid copies for replication has to be considered when models for control of replication are constructed.", "contents": "Random replication of the stringent plasmid R1 in Escherichia coli K-12. The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The results are in quantitative accordance with a random replication of R1 in which the replicating molecules are taken from a cytoplasmic plasmid pool and transferred back to the pool after replication. This is analogous to the results obtained by Bazaral and Helinski (1970) and Rownd (1969) for plasmids that are present in 10 to 20 copies per genome (so-called relaxed control of replication). Hence, there seem to be no difference between stringent and relaxed plasmids with respect to selection of plasmid molecules for replication. However, we cannot tell whether all R1 copies in a cell replicate during a fraction of or throughout the cell cycle. The random selction of plasmid copies for replication has to be considered when models for control of replication are constructed."}
{"id": "PMID:1097411", "title": "Ribonucleic acid destruction and synthesis during intraperiplasmic growth of Bdellovibrio bacteriovorus.", "content": "During growth of Bdellovibrio bacteriovorus on (2-14C)uracil-labeled Escherichia coli approximately 50% of the radioactivity is incorporated by the bdellovibrio and most of the remainder is released as free nucleic acid bases. Kinetic studies showed that 50 and 30S ribosomal particles and 23 and 16S ribosomal ribonucleic acid (RNA) of E. coli are almost completely degraded by the first 90 min in a 210- to 240-min bdellovibrio developmental cycle. Synthesis of bdellovibrio ribosomal RNA was first detected after 90 min. The specific activity and the ratio of radioactivity in the bases of the synthesized bdellovibrio RNA was essentially the same as those of the substrate E. coli. The total radioactivity of the bdellovibrio deoxyribonucleic acid (DNA) exceeded that in the DNA of the substrate E. coli cell, and the ratio of radioactivity of cytosine to thymine residues differed. Intraperiplasmic growth of B. bacteriovorus in the presence of added nucleoside monophosphates (singly or in combination) significantly decreased the uptake of radioactivity from (2-14C)uracil-labeled E. coli; nucleosides or nucleic acid bases did not. It is concluded that the RNA of the substrate cell, in the form of nucleoside monophosphates, is the major or exclusive precursor of the bdellovirbrio RNA and also serves as a precursor for some of the bdellovibrio DNA.", "contents": "Ribonucleic acid destruction and synthesis during intraperiplasmic growth of Bdellovibrio bacteriovorus. During growth of Bdellovibrio bacteriovorus on (2-14C)uracil-labeled Escherichia coli approximately 50% of the radioactivity is incorporated by the bdellovibrio and most of the remainder is released as free nucleic acid bases. Kinetic studies showed that 50 and 30S ribosomal particles and 23 and 16S ribosomal ribonucleic acid (RNA) of E. coli are almost completely degraded by the first 90 min in a 210- to 240-min bdellovibrio developmental cycle. Synthesis of bdellovibrio ribosomal RNA was first detected after 90 min. The specific activity and the ratio of radioactivity in the bases of the synthesized bdellovibrio RNA was essentially the same as those of the substrate E. coli. The total radioactivity of the bdellovibrio deoxyribonucleic acid (DNA) exceeded that in the DNA of the substrate E. coli cell, and the ratio of radioactivity of cytosine to thymine residues differed. Intraperiplasmic growth of B. bacteriovorus in the presence of added nucleoside monophosphates (singly or in combination) significantly decreased the uptake of radioactivity from (2-14C)uracil-labeled E. coli; nucleosides or nucleic acid bases did not. It is concluded that the RNA of the substrate cell, in the form of nucleoside monophosphates, is the major or exclusive precursor of the bdellovirbrio RNA and also serves as a precursor for some of the bdellovibrio DNA."}
{"id": "PMID:1097412", "title": "Induction of lactose transport in Escherichia coli during the absence of phospholipid synthesis.", "content": "Induction of lactose transport and of beta-galactosidase synthesis was examined in two Escherichia coli strains that require exogenous glycerol for phospholipid synthesis and growth. No preferential inhibition of lactose transport induction was observed when phospholipid synthesis was restricted to 5 to 10% of the normal rate. We conclude that the lactose transport system does not require concurrent phospholipid synthesis for its functional assembly.", "contents": "Induction of lactose transport in Escherichia coli during the absence of phospholipid synthesis. Induction of lactose transport and of beta-galactosidase synthesis was examined in two Escherichia coli strains that require exogenous glycerol for phospholipid synthesis and growth. No preferential inhibition of lactose transport induction was observed when phospholipid synthesis was restricted to 5 to 10% of the normal rate. We conclude that the lactose transport system does not require concurrent phospholipid synthesis for its functional assembly."}
{"id": "PMID:1097413", "title": "Nuclear and mitochondrial deoxyribonucleic acid replication during mitosis in Saccharomyces cerevisiae.", "content": "To study nuclear and mitochondrial deoxyribonucleic acid (DNA) synthesis during the cell cycle, a 15N-labeled log-phase population of Saccharomyces cervisiae was shifted to 14N medium. After one-half generation, the cells were centrifuged on a sorbitol gradient in a zonal rotor to fractionate the population according to cell size and age into fractions representing the yeast cell cycle. DNA samples isolated from the zonal rotor cell samples were centrifuged to equilibrium in CsC1 in an analytical ultracentrifuge to separate the nuclear and mitochondrial DNA components. The amount of 14N incorporated into each 15N-labeled DNA species was measured. The extent of nuclear DNA replication per sample was obtained by measuring the amount of hybrid DNA. The percentage of hybrid nuclear DNA increased from 6 to 68% and then decreased to 44% during the cell cycle. Upon ultracentrifugation, mitochondrial DNA banded as a unimodal peak in all zonal rotor samples. Mitochondrial DNA replication could be ascertained only by the 14N level in each mitochondrial peak and not, as with nuclear DNA, by hybrid DNA level. In contrast to the nuclear incorporation pattern, the 14N percentage in mitochondrial DNA remained effectively constant during the cell cycle. Comparison of the data to theoretical distributions showed that nuclear DNA was replicated discontinuously during the cell cycle, whereas mitochondrial DNA was replicated continuously throughout the entire mitotic cycle.", "contents": "Nuclear and mitochondrial deoxyribonucleic acid replication during mitosis in Saccharomyces cerevisiae. To study nuclear and mitochondrial deoxyribonucleic acid (DNA) synthesis during the cell cycle, a 15N-labeled log-phase population of Saccharomyces cervisiae was shifted to 14N medium. After one-half generation, the cells were centrifuged on a sorbitol gradient in a zonal rotor to fractionate the population according to cell size and age into fractions representing the yeast cell cycle. DNA samples isolated from the zonal rotor cell samples were centrifuged to equilibrium in CsC1 in an analytical ultracentrifuge to separate the nuclear and mitochondrial DNA components. The amount of 14N incorporated into each 15N-labeled DNA species was measured. The extent of nuclear DNA replication per sample was obtained by measuring the amount of hybrid DNA. The percentage of hybrid nuclear DNA increased from 6 to 68% and then decreased to 44% during the cell cycle. Upon ultracentrifugation, mitochondrial DNA banded as a unimodal peak in all zonal rotor samples. Mitochondrial DNA replication could be ascertained only by the 14N level in each mitochondrial peak and not, as with nuclear DNA, by hybrid DNA level. In contrast to the nuclear incorporation pattern, the 14N percentage in mitochondrial DNA remained effectively constant during the cell cycle. Comparison of the data to theoretical distributions showed that nuclear DNA was replicated discontinuously during the cell cycle, whereas mitochondrial DNA was replicated continuously throughout the entire mitotic cycle."}
{"id": "PMID:1097414", "title": "Mating aggregates in Escherichia coli conjugation.", "content": "Mating mixtures of Escherichia coli cells were shown to contain mating aggregates of two to 20 cells each rather than only mating pairs of two cells each. The mating aggregate size distribution shows two broad peaks, at two to four cells and at eight to 13 cells. The quantitative mating aggregate size distribution and the proportion of male cells in mating aggregates are dependent on the input ratio of male to female cells. At an input ratio of one to one, the average mating aggregate contains equal proportions of male and female cells and most of the cells involved in mating are in large aggregates of seven or more cells each. The deoxyribonucleic acid (DNA) transfer efficiency per mating aggregate cell was constant regardless of average aggregate size or of the ratio of male to female cells in the aggregate. Under optimal conditions essentially every male cell or every female cell in a mating aggregate can be involved in DNA transfer. A comparison of light microscopy, sucrose gradient centrifugation, and analysis with a modified Coulter counter indicated that the number of cells in mating aggregates is best equantitated using a modified Coulter counter.", "contents": "Mating aggregates in Escherichia coli conjugation. Mating mixtures of Escherichia coli cells were shown to contain mating aggregates of two to 20 cells each rather than only mating pairs of two cells each. The mating aggregate size distribution shows two broad peaks, at two to four cells and at eight to 13 cells. The quantitative mating aggregate size distribution and the proportion of male cells in mating aggregates are dependent on the input ratio of male to female cells. At an input ratio of one to one, the average mating aggregate contains equal proportions of male and female cells and most of the cells involved in mating are in large aggregates of seven or more cells each. The deoxyribonucleic acid (DNA) transfer efficiency per mating aggregate cell was constant regardless of average aggregate size or of the ratio of male to female cells in the aggregate. Under optimal conditions essentially every male cell or every female cell in a mating aggregate can be involved in DNA transfer. A comparison of light microscopy, sucrose gradient centrifugation, and analysis with a modified Coulter counter indicated that the number of cells in mating aggregates is best equantitated using a modified Coulter counter."}
{"id": "PMID:1097415", "title": "Specificity and genetics of S-adenosylmethionine transport in Saccharomyces cerevisiae.", "content": "The specificity of a transport system for S-adenosylmethionine was determined through the use of structurally related derivatives. Of the compounds tested, the analogues S-adenosylethionine and S-inosylmethionine and the naturally occurring compounds S-adenosyl-(5')-3-methylthiopropylamine and S-adenosylhomocysteine competitively inhibited uptake of the sulfonium compound. Ki values for these compounds indicate that the order of affinity for the transport protein is S-adenosylmethionine congruent to S-adenosyl-(5')-3-methyl-thiopropylamine greater than S-adenosylethionine greater than S-inosylmethionine greater than S-adenosylhomocysteins. S-adenosyl-(2-hydroxy-4-methylthio)butyric acid exerted inhibition of a mixed type. S-insoyl-(2-hydroxy-4-methylthio)butyric acid, S-inosylhomocysteine, and S-ribosylhomocysteine were without effect. On the basis of the inhibition data, the methionine-amino, adenine-amino, and methyl groups were identified as group important in the binding of S-adenosylmethionine to the transport protein. Comparison is made with the specificities of various transmethylating enzymes utilizing S-adenosylmethionine. In addition, a number of conventional and temperature-sensitive S-adenosylmethionine transport mutants were isolated and analyzed in an attempt to identify the structural character of the specific transport protein(s). The data obtained suggest that only a single gene (a single polypeptide) is involved in specific S-adenosylmethionine transport. Apparent interallelic complementation supports the assumption that the functional form of the protein is composed of two or more copies of a monomer.", "contents": "Specificity and genetics of S-adenosylmethionine transport in Saccharomyces cerevisiae. The specificity of a transport system for S-adenosylmethionine was determined through the use of structurally related derivatives. Of the compounds tested, the analogues S-adenosylethionine and S-inosylmethionine and the naturally occurring compounds S-adenosyl-(5')-3-methylthiopropylamine and S-adenosylhomocysteine competitively inhibited uptake of the sulfonium compound. Ki values for these compounds indicate that the order of affinity for the transport protein is S-adenosylmethionine congruent to S-adenosyl-(5')-3-methyl-thiopropylamine greater than S-adenosylethionine greater than S-inosylmethionine greater than S-adenosylhomocysteins. S-adenosyl-(2-hydroxy-4-methylthio)butyric acid exerted inhibition of a mixed type. S-insoyl-(2-hydroxy-4-methylthio)butyric acid, S-inosylhomocysteine, and S-ribosylhomocysteine were without effect. On the basis of the inhibition data, the methionine-amino, adenine-amino, and methyl groups were identified as group important in the binding of S-adenosylmethionine to the transport protein. Comparison is made with the specificities of various transmethylating enzymes utilizing S-adenosylmethionine. In addition, a number of conventional and temperature-sensitive S-adenosylmethionine transport mutants were isolated and analyzed in an attempt to identify the structural character of the specific transport protein(s). The data obtained suggest that only a single gene (a single polypeptide) is involved in specific S-adenosylmethionine transport. Apparent interallelic complementation supports the assumption that the functional form of the protein is composed of two or more copies of a monomer."}
{"id": "PMID:1097416", "title": "Genes for ribitol and D-arabitol catabolism in Escherichia coli: their loci in C strains and absence in K-12 and B strains.", "content": "Escherichia coli C strains can grow at the expense of the two natural pentitols ribitol and D-arabitol, sugar alcohols previously thought not to be utilized by E. coli. E. coli strains K-12 and B cannot utilize either compound. The genetic loci responsible for pentitol catabolism in E. coli C, designated rtl and atl, are separate and closely linked. Each lies between metG and his and is highly co-transducible with metG and with a P2 prophage attachment site. rtl and atl readily can be transduced into E. coli K-12 or B strains, in which they integrate at, or very near, their E. coli C location. Transduction also can be used to insert rtl and atl into certain E. coli K-12 F' plasmids. No recombination between E. coli C strains and either K-12 or B strains occurs within the rtl-atl genetic region after interstrain conjugations or transductions. No cryptic rtl or atl genes in K-12 or B strains can be detected by complementation, recombination, or mutagenesis. These results are consistent with the view that the rtl-atl portion of the E. coli C chromosome has no counterpart in E. coli K-12 or B and may have been obtained from an extrageneric source. Detailed biochemical and genetic comparisons of penitol utilization in E. coli and Klebsiella aerogenes are in progress. The ability to catabolize xylitol is conferred upon E. coli C strains by a mutation at or adjacent to the rtl locus, whereas in E. coli K-12 or B strains harboring rtl an additional mutation at a separate locus is required for xylitol utilization.", "contents": "Genes for ribitol and D-arabitol catabolism in Escherichia coli: their loci in C strains and absence in K-12 and B strains. Escherichia coli C strains can grow at the expense of the two natural pentitols ribitol and D-arabitol, sugar alcohols previously thought not to be utilized by E. coli. E. coli strains K-12 and B cannot utilize either compound. The genetic loci responsible for pentitol catabolism in E. coli C, designated rtl and atl, are separate and closely linked. Each lies between metG and his and is highly co-transducible with metG and with a P2 prophage attachment site. rtl and atl readily can be transduced into E. coli K-12 or B strains, in which they integrate at, or very near, their E. coli C location. Transduction also can be used to insert rtl and atl into certain E. coli K-12 F' plasmids. No recombination between E. coli C strains and either K-12 or B strains occurs within the rtl-atl genetic region after interstrain conjugations or transductions. No cryptic rtl or atl genes in K-12 or B strains can be detected by complementation, recombination, or mutagenesis. These results are consistent with the view that the rtl-atl portion of the E. coli C chromosome has no counterpart in E. coli K-12 or B and may have been obtained from an extrageneric source. Detailed biochemical and genetic comparisons of penitol utilization in E. coli and Klebsiella aerogenes are in progress. The ability to catabolize xylitol is conferred upon E. coli C strains by a mutation at or adjacent to the rtl locus, whereas in E. coli K-12 or B strains harboring rtl an additional mutation at a separate locus is required for xylitol utilization."}
{"id": "PMID:1097417", "title": "Intrinsic and extrinsic light responses of Salmonella typhimurium and Escherichia coli.", "content": "Exposure to intense light in the region between 390 and 530 nm has been shown to have three effects on the motility of Salmonella typhimurium and Escherichia coli. Short pulses of light initiate continuous tumbling. Longer exposures to light induce smooth swimming, and prolonged exposures induced paralysis. The tumbling response is intimately connected with the chemical gradient-sensing apparatus of the bacterium and can be overcome by strong temporal gradients of attractant. Some mutants of S. typhimurium which are defective in the tumble-generating mechanism for chemotaxis are also unable to tumble in intense light. This intrinsic light effect can be mimicked by the addition of external dyes (the classical photodynamic effect), but it can be shown that the two phenomena are distinct. The extrinsic (photodynamic) effect can be inhibited by histidine or by anaerobic conditions, whereas the intrinsic effect is not. The observation that the extrinsic effect can also produce the three types of light responses listed above suggests a common pathway after an intial event on either an endogenous or an externally added photoreceptor.", "contents": "Intrinsic and extrinsic light responses of Salmonella typhimurium and Escherichia coli. Exposure to intense light in the region between 390 and 530 nm has been shown to have three effects on the motility of Salmonella typhimurium and Escherichia coli. Short pulses of light initiate continuous tumbling. Longer exposures to light induce smooth swimming, and prolonged exposures induced paralysis. The tumbling response is intimately connected with the chemical gradient-sensing apparatus of the bacterium and can be overcome by strong temporal gradients of attractant. Some mutants of S. typhimurium which are defective in the tumble-generating mechanism for chemotaxis are also unable to tumble in intense light. This intrinsic light effect can be mimicked by the addition of external dyes (the classical photodynamic effect), but it can be shown that the two phenomena are distinct. The extrinsic (photodynamic) effect can be inhibited by histidine or by anaerobic conditions, whereas the intrinsic effect is not. The observation that the extrinsic effect can also produce the three types of light responses listed above suggests a common pathway after an intial event on either an endogenous or an externally added photoreceptor."}
{"id": "PMID:1097418", "title": "Enhanced level and metabolic regulation of methionyl-transfer ribonucleic acid synthetase in different strains of Escherichia coli K-12.", "content": "The methionyl-transfer ribonucleic acid (tRNA) synthetase of Escherichia coli K-12 eductants carrying P2-mediated deletions in the region of the structural gene of this enzyme was investigated. No structural alteration of this enzyme was observed in three eductants examined. These were isolated from strain AB311, which had a threefold higher level of methionyl-tRNA synthetase than most haploid strains examined. In two of the three eductants studied, the level of this enzyme was twofold higher than in their parental strain regardless of growth conditions used. In contrast, isoleucyl-, leucyl-, and valyl-tRNA synthetases had similar levels in all strains examined. Like valyl-tRNA synthetase, but to a lesser extent, methionyl-tRNA synthetase was subject to metabolic regulation. Coupling between the level of methionyl-tRNA synthetase and growth rate was observed even in strains that had an enhanced level of methionyl-tRNA synthetase. These results suggest that the formation of methionyl-tRNA synthetase remains subject to metabolic regulation even when the repression-like mechanism that controls the synthesis of this enzyme is altered. In addition, we report that in the merodiploid strain EM20031, which was haploid for the valyl-tRNA synthetase structural gene and diploid for the structural genes of methionyl-tRNA synthetase and D-serine deaminase, the levels of these latter two enzymes varied to a minor yet significant extent with the phosphate concentration of the culture medium; under the same conditions, the level of valyl-tRNA synthetase remained unchanged. Moreover, no variation of the levels of these three enzymes in response to phosphate was observed in the haploid strain HfrH. These results indicate that in the merodiploid strain EM20031, which carries the episome F32, the number of episomes per chromosome varies to some extent according to the phosphate concentration of the culture medium.", "contents": "Enhanced level and metabolic regulation of methionyl-transfer ribonucleic acid synthetase in different strains of Escherichia coli K-12. The methionyl-transfer ribonucleic acid (tRNA) synthetase of Escherichia coli K-12 eductants carrying P2-mediated deletions in the region of the structural gene of this enzyme was investigated. No structural alteration of this enzyme was observed in three eductants examined. These were isolated from strain AB311, which had a threefold higher level of methionyl-tRNA synthetase than most haploid strains examined. In two of the three eductants studied, the level of this enzyme was twofold higher than in their parental strain regardless of growth conditions used. In contrast, isoleucyl-, leucyl-, and valyl-tRNA synthetases had similar levels in all strains examined. Like valyl-tRNA synthetase, but to a lesser extent, methionyl-tRNA synthetase was subject to metabolic regulation. Coupling between the level of methionyl-tRNA synthetase and growth rate was observed even in strains that had an enhanced level of methionyl-tRNA synthetase. These results suggest that the formation of methionyl-tRNA synthetase remains subject to metabolic regulation even when the repression-like mechanism that controls the synthesis of this enzyme is altered. In addition, we report that in the merodiploid strain EM20031, which was haploid for the valyl-tRNA synthetase structural gene and diploid for the structural genes of methionyl-tRNA synthetase and D-serine deaminase, the levels of these latter two enzymes varied to a minor yet significant extent with the phosphate concentration of the culture medium; under the same conditions, the level of valyl-tRNA synthetase remained unchanged. Moreover, no variation of the levels of these three enzymes in response to phosphate was observed in the haploid strain HfrH. These results indicate that in the merodiploid strain EM20031, which carries the episome F32, the number of episomes per chromosome varies to some extent according to the phosphate concentration of the culture medium."}
{"id": "PMID:1097419", "title": "Role of methionyl-transfer ribonucleic acid in the regulation of methionyl-transfer ribonucleic acid synthetase of Escherichia coli K-12.", "content": "A decrease in the in vivo acylation level of methionine transfer ribonucleic acid (tRNAmet) induced by methioninyl adenylate led to a specific derepression of methionyl-transfer ribonucleic acid (tRNA) synthetase formation. This derepression required de novo protein synthesis and was reflected by overproduction of unaltered enzyme. Two different strains of Escherichia coli K-12 that have normal levels of methionyl-tRNA synthetase were examined and the derepression of methionyl-tRNA synthetase was observed in both. Moreover, for one of these strains, the relation between the level of methionyl-tRNA synthetase and deacylation level of tRNAmet was established; under the growth conditions used, when more than 25% of tRNAmet was deacylated, methionyl-tRNA synthetase formation was derepressed and the level of derepression became proportional to the amount of tRNAmet deacylated. Concomitantly, the enzyme was subject to specific inactivation as a consequence of which the true de novo rate of derepression of the formation of this enzyme was higher than that determined by measurements of enzyme activity. These studies were extended to strains AB311 and ed2, which had a constitutive enhanced level of methionyl-tRNA synthetase. In these strains no derepression of enzyme formation was observed on reducing the acylation level of tRNAmet by use of methioninyl adenylate.", "contents": "Role of methionyl-transfer ribonucleic acid in the regulation of methionyl-transfer ribonucleic acid synthetase of Escherichia coli K-12. A decrease in the in vivo acylation level of methionine transfer ribonucleic acid (tRNAmet) induced by methioninyl adenylate led to a specific derepression of methionyl-transfer ribonucleic acid (tRNA) synthetase formation. This derepression required de novo protein synthesis and was reflected by overproduction of unaltered enzyme. Two different strains of Escherichia coli K-12 that have normal levels of methionyl-tRNA synthetase were examined and the derepression of methionyl-tRNA synthetase was observed in both. Moreover, for one of these strains, the relation between the level of methionyl-tRNA synthetase and deacylation level of tRNAmet was established; under the growth conditions used, when more than 25% of tRNAmet was deacylated, methionyl-tRNA synthetase formation was derepressed and the level of derepression became proportional to the amount of tRNAmet deacylated. Concomitantly, the enzyme was subject to specific inactivation as a consequence of which the true de novo rate of derepression of the formation of this enzyme was higher than that determined by measurements of enzyme activity. These studies were extended to strains AB311 and ed2, which had a constitutive enhanced level of methionyl-tRNA synthetase. In these strains no derepression of enzyme formation was observed on reducing the acylation level of tRNAmet by use of methioninyl adenylate."}
{"id": "PMID:1097420", "title": "Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae.", "content": "Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall locus. The mn4 locus, which regulates the synthesis of mannosylphosphate groups on the mannan side chains, was placed on chromosome XI near trp3 and ural and a locus previously reported to regulate the ability of a S. diastaticus strain to bind alcian blue (Friis and Ottolenghi, 1970). The mnn4 mutant also fails to bind alcain blue, but the gene responsible for alcian blud binding in this strain segregates independently from the dye-binding locus of S. diastaticus, and therefore must be a different gene. A diploid heterozygous for mnn4 fails to bind dye, indicating dominance of this mutant genotype. The alcian blue dye binding locus dbll, reported to Friis and Ottolenghi (1970), is also dominant. Thus, there are at least two independent genes that control the formation of the mannosylphosphate units in the mannan side chains, and both have the property of dominance in the mutant form.", "contents": "Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae. Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall locus. The mn4 locus, which regulates the synthesis of mannosylphosphate groups on the mannan side chains, was placed on chromosome XI near trp3 and ural and a locus previously reported to regulate the ability of a S. diastaticus strain to bind alcian blue (Friis and Ottolenghi, 1970). The mnn4 mutant also fails to bind alcain blue, but the gene responsible for alcian blud binding in this strain segregates independently from the dye-binding locus of S. diastaticus, and therefore must be a different gene. A diploid heterozygous for mnn4 fails to bind dye, indicating dominance of this mutant genotype. The alcian blue dye binding locus dbll, reported to Friis and Ottolenghi (1970), is also dominant. Thus, there are at least two independent genes that control the formation of the mannosylphosphate units in the mannan side chains, and both have the property of dominance in the mutant form."}
{"id": "PMID:1097421", "title": "Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1 drd-19B2.", "content": "The large mutant R-factor R1drd-19B2 gives rise to several classes of small, covalently closed circular deoxyribonucleic acids (DNAs), designated as Rsc DNAs, when harbored by the K-12 strain CRT46 which carries a dnaA mutation. The molecular weights of these DNA molecules range from 3 X 106 to 8.4 X 106. Cells arising from single colonies of CRT46-R1drd-19B2 harbor only one to two copies of the large mutant R-factor and in addition 10 to 20 copies of Rsc plasmid of a discrete size class per chromosome. The larger Rsc DNAs carry the ampicillin resistance gene. After transformation the small circular DNAs are present in Escherichia coli C in a large number of copies, up to 100 copies per chromosome. Hybridization studies between Rsc plasmids indicate that they possess common DNA sequences.", "contents": "Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1 drd-19B2. The large mutant R-factor R1drd-19B2 gives rise to several classes of small, covalently closed circular deoxyribonucleic acids (DNAs), designated as Rsc DNAs, when harbored by the K-12 strain CRT46 which carries a dnaA mutation. The molecular weights of these DNA molecules range from 3 X 106 to 8.4 X 106. Cells arising from single colonies of CRT46-R1drd-19B2 harbor only one to two copies of the large mutant R-factor and in addition 10 to 20 copies of Rsc plasmid of a discrete size class per chromosome. The larger Rsc DNAs carry the ampicillin resistance gene. After transformation the small circular DNAs are present in Escherichia coli C in a large number of copies, up to 100 copies per chromosome. Hybridization studies between Rsc plasmids indicate that they possess common DNA sequences."}
{"id": "PMID:1097422", "title": "alphabeta sequence of F is IS31.", "content": "Previous studies have shown that there is a deoxyribonucleic acid (DNA) segment, of length 1.3 kb and denoted as the alphabeta sequence, which occurs twice on the F plasmid at corrdinates 93.2 to 94.5/OF kb and 13.7 to 15.0F kb. In the present investigation, heteroduplexes were prepared between a phage DNA carrying the insertion sequence IS3 and suitable F-prime DNAs. The hybrids formed show that IS3 is the same as alphabeta. This result plus previous studies support the view that: (i) the insertion sequence IS2 and IS3 occur on F and, in multiple copies, on the main bacterial chromosome of Escherichia coli K-12; and (ii)these IS sequences on the main bacterial chromosomes are hot spots for Hfr formation by reciprocal recombination with the corresponding sequences of F.", "contents": "alphabeta sequence of F is IS31. Previous studies have shown that there is a deoxyribonucleic acid (DNA) segment, of length 1.3 kb and denoted as the alphabeta sequence, which occurs twice on the F plasmid at corrdinates 93.2 to 94.5/OF kb and 13.7 to 15.0F kb. In the present investigation, heteroduplexes were prepared between a phage DNA carrying the insertion sequence IS3 and suitable F-prime DNAs. The hybrids formed show that IS3 is the same as alphabeta. This result plus previous studies support the view that: (i) the insertion sequence IS2 and IS3 occur on F and, in multiple copies, on the main bacterial chromosome of Escherichia coli K-12; and (ii)these IS sequences on the main bacterial chromosomes are hot spots for Hfr formation by reciprocal recombination with the corresponding sequences of F."}
{"id": "PMID:1097423", "title": "Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation.", "content": "Mutations ts2158 and ts1882, which confer temperature sensitivity of septum formation, map near leu in the region of min 2.0 to 2.1 on the Escherichia coli chromosome. These mutants stop division abruptly and grow as filaments at 42 C; when returned to 28 C, division resumes after about 30 min to produce short cells. The product of the gene defined by these mutations probably is required during all stages of septum formation rather than specifically for initiation of septation. Filaments that formed at 42 C contained incomplete constrictiions (septa). When actively dividing filaments (i.e., those incubated at 28 C until division resumed) were shifted to 42 C a second time, division again stopped abruptly and incomplete constrictions persisted during the incubation at 42 C. Filaments that were subjected to 28 C incubation for a brief time (10, 20, or 30 min) before being shifted again to 42 C did not resume division as would be expected of a strain defective in initiating septation. Mutations ts 1882 and ts2158 and recessive to the ts+ allele, which is consistent with the interpretation that these mutations cause the loss of a function. They did not complement each other and presumably represent one cistron. Mutants carrying ts1882 and ts2158 mutations were compared with a mutant defective in the ftA allele, also known to map near leu.", "contents": "Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation. Mutations ts2158 and ts1882, which confer temperature sensitivity of septum formation, map near leu in the region of min 2.0 to 2.1 on the Escherichia coli chromosome. These mutants stop division abruptly and grow as filaments at 42 C; when returned to 28 C, division resumes after about 30 min to produce short cells. The product of the gene defined by these mutations probably is required during all stages of septum formation rather than specifically for initiation of septation. Filaments that formed at 42 C contained incomplete constrictiions (septa). When actively dividing filaments (i.e., those incubated at 28 C until division resumed) were shifted to 42 C a second time, division again stopped abruptly and incomplete constrictions persisted during the incubation at 42 C. Filaments that were subjected to 28 C incubation for a brief time (10, 20, or 30 min) before being shifted again to 42 C did not resume division as would be expected of a strain defective in initiating septation. Mutations ts 1882 and ts2158 and recessive to the ts+ allele, which is consistent with the interpretation that these mutations cause the loss of a function. They did not complement each other and presumably represent one cistron. Mutants carrying ts1882 and ts2158 mutations were compared with a mutant defective in the ftA allele, also known to map near leu."}
{"id": "PMID:1097424", "title": "Discontinuous replication of colicin E1 plasmid deoxyribonucleic acid.", "content": "The plasmid pML 21, which was found to contain approximately 49% of the Col E1 genome was used to determine the template origin of single-stranded deoxyribonucleic acid (DNA) fragments (4 to 32% of the Col E1 units length) associated with Col E1 dna replicative intermediates. The results of DNA hybridization competition experiments indicate that the single-stranded fragments derive from the full length of the Col E1 DNA template as expected for Okazaki fragments and the plasmid pML 21 contains the replication origin of Col E1 DNA.", "contents": "Discontinuous replication of colicin E1 plasmid deoxyribonucleic acid. The plasmid pML 21, which was found to contain approximately 49% of the Col E1 genome was used to determine the template origin of single-stranded deoxyribonucleic acid (DNA) fragments (4 to 32% of the Col E1 units length) associated with Col E1 dna replicative intermediates. The results of DNA hybridization competition experiments indicate that the single-stranded fragments derive from the full length of the Col E1 DNA template as expected for Okazaki fragments and the plasmid pML 21 contains the replication origin of Col E1 DNA."}
{"id": "PMID:1097425", "title": "Unusual sensitivity of Escherichia coli to adenine or adenine plus histidine.", "content": "The W3110 strain of Escherichia coli K-12 is unusually sensitive to adenine. Inhibition of growth is relieved by a combination of thiamine and uridine (or cytidine). In the presence of histidine, inhibition is more severe and is relieved by a combination of thiamine, glycine, uridine (or cytidine), and inosine (or guanosine).", "contents": "Unusual sensitivity of Escherichia coli to adenine or adenine plus histidine. The W3110 strain of Escherichia coli K-12 is unusually sensitive to adenine. Inhibition of growth is relieved by a combination of thiamine and uridine (or cytidine). In the presence of histidine, inhibition is more severe and is relieved by a combination of thiamine, glycine, uridine (or cytidine), and inosine (or guanosine)."}
{"id": "PMID:1097426", "title": "Strain-specific difference that affects the inhibition of division of Escherichia coli filaments by chloramphenicol.", "content": "The Escherichia coli mutations ts1882 and ts2158 cause temperature-sensitive septum formation and result in growth of cells as long, multinucleate, nonseptate filaments at 42 C. When filaments are transferred to 28 C, they divide into short cells. Chloramphenicol, when added to cultures of filaments at the time of temperature reduction. Inhibited division of filaments when these temperature-sensitive mutations were present in the K-12 strain AB1157. However, when the ts1882 and ts2158 mutations were present in another k-12 strain, UTH4113, filaments of these strains divided in the presence of chloramphenicol.", "contents": "Strain-specific difference that affects the inhibition of division of Escherichia coli filaments by chloramphenicol. The Escherichia coli mutations ts1882 and ts2158 cause temperature-sensitive septum formation and result in growth of cells as long, multinucleate, nonseptate filaments at 42 C. When filaments are transferred to 28 C, they divide into short cells. Chloramphenicol, when added to cultures of filaments at the time of temperature reduction. Inhibited division of filaments when these temperature-sensitive mutations were present in the K-12 strain AB1157. However, when the ts1882 and ts2158 mutations were present in another k-12 strain, UTH4113, filaments of these strains divided in the presence of chloramphenicol."}
{"id": "PMID:1097427", "title": "Amino-terminal sequences of indoleglycerol phosphate synthetase of Escherichia coli and Salmonella typhimurium.", "content": "The partial sequences of the first 40 residues of indoleglycerol phosphate synthetase of Escherichia coli and Salmonella typhimurium were determined, and three amino acid differences were observed among the 38 residues compared.", "contents": "Amino-terminal sequences of indoleglycerol phosphate synthetase of Escherichia coli and Salmonella typhimurium. The partial sequences of the first 40 residues of indoleglycerol phosphate synthetase of Escherichia coli and Salmonella typhimurium were determined, and three amino acid differences were observed among the 38 residues compared."}
{"id": "PMID:1097428", "title": "Analysis of bacteriophage deoxyribonucleic acid sequences methylated by host- and R-factor-controlled enzymes.", "content": "Phages lambda and fd were propagated in Escherichia coli strains that have either host K-12 or the N-3 R-factor deoxyribonucleic acid-cytosine methylase activity. Pyrimidine tracts containing 3H-labeled 5-methylcytosine (MeC) were analyzed; in all cases, the major methylated sequence was 5' ... C-MeC-T ... 3'.", "contents": "Analysis of bacteriophage deoxyribonucleic acid sequences methylated by host- and R-factor-controlled enzymes. Phages lambda and fd were propagated in Escherichia coli strains that have either host K-12 or the N-3 R-factor deoxyribonucleic acid-cytosine methylase activity. Pyrimidine tracts containing 3H-labeled 5-methylcytosine (MeC) were analyzed; in all cases, the major methylated sequence was 5' ... C-MeC-T ... 3'."}
{"id": "PMID:1097429", "title": "Effect of tsl mutations on Col E1 expression in a recA strain of Escherichia coli K-12.", "content": "Spontaneous colicin production by Col E1 in a recA- strain of Escherichia coli K-12 is restored nearly to wild-type levels by the presence of tsl- second site mutation.", "contents": "Effect of tsl mutations on Col E1 expression in a recA strain of Escherichia coli K-12. Spontaneous colicin production by Col E1 in a recA- strain of Escherichia coli K-12 is restored nearly to wild-type levels by the presence of tsl- second site mutation."}
{"id": "PMID:1097430", "title": "Hybridization of glyceraldehyde-3-phosphate dehydrogenase.", "content": "1. Glyceraldehyde-3-phosphate dehydrogenases [EC 1.2.1.12] from rabbit, pig, lobster, yeast, E. coli, and B. stearothermophilus have been subjected to hybridization in 3M NaCL. 2. Suitable mixtures of electrophoretically distinct glyceraldehyde-3-phosphate dehydrogenases were found to give five-membered by electrophoresis on cellulose acetate. 3. The thermophile enzyme did not form hybrids with any of its mesophile counterparts, presumably because it does not dissociate under the conditions used. 4. Hybridization of pig enzyme with lobster enzyme that had been inactivated by selective carboxymethylation of Cystetramer containing only one active pig enzyme subunit. Individual subunits would thus appear to express their activity independently even within hybrid tetramers formed with subunits of another species. 5. The successful hybridization of glyceraldehyde-3-phosphate dehydrogenases from evolutionarily distant sources suggests that the tertiary and quaternary structures of the enzyme have been highly conserved.", "contents": "Hybridization of glyceraldehyde-3-phosphate dehydrogenase. 1. Glyceraldehyde-3-phosphate dehydrogenases [EC 1.2.1.12] from rabbit, pig, lobster, yeast, E. coli, and B. stearothermophilus have been subjected to hybridization in 3M NaCL. 2. Suitable mixtures of electrophoretically distinct glyceraldehyde-3-phosphate dehydrogenases were found to give five-membered by electrophoresis on cellulose acetate. 3. The thermophile enzyme did not form hybrids with any of its mesophile counterparts, presumably because it does not dissociate under the conditions used. 4. Hybridization of pig enzyme with lobster enzyme that had been inactivated by selective carboxymethylation of Cystetramer containing only one active pig enzyme subunit. Individual subunits would thus appear to express their activity independently even within hybrid tetramers formed with subunits of another species. 5. The successful hybridization of glyceraldehyde-3-phosphate dehydrogenases from evolutionarily distant sources suggests that the tertiary and quaternary structures of the enzyme have been highly conserved."}
{"id": "PMID:1097431", "title": "Preferential production of IgG2 anti-hapten antibody by immunization with hapten-conjugated Escherichia coli.", "content": "It is well-known that immunization of guinea pigs with hapten-protein conjugates induces concomitant production of IgG1 and IgG2 anti-hapten antibodies.  However, the synthesis of antibody to 2, 4-dinitrophenyl groups (DNP) was found to be restricted to one of the IgG antibodies (IgG2 antibody) when guinea pigs were immunized through repeated intraperitoneal injections of 0.1 mg of 2, 4-dinitrophenylated Escherichia coli (DNP-E coli).  This selective induction of IgG2 anti-DNP antibody formation occurred both in the presence and absence of Freund's adjuvants, whereas a trace of IgG1 anti-DNP antibody was produced concomitantly on increasing the immunizing dose of DNP-E. coli (3.0 mg) in Freund's complete adjuvant (FAC).", "contents": "Preferential production of IgG2 anti-hapten antibody by immunization with hapten-conjugated Escherichia coli. It is well-known that immunization of guinea pigs with hapten-protein conjugates induces concomitant production of IgG1 and IgG2 anti-hapten antibodies.  However, the synthesis of antibody to 2, 4-dinitrophenyl groups (DNP) was found to be restricted to one of the IgG antibodies (IgG2 antibody) when guinea pigs were immunized through repeated intraperitoneal injections of 0.1 mg of 2, 4-dinitrophenylated Escherichia coli (DNP-E coli).  This selective induction of IgG2 anti-DNP antibody formation occurred both in the presence and absence of Freund's adjuvants, whereas a trace of IgG1 anti-DNP antibody was produced concomitantly on increasing the immunizing dose of DNP-E. coli (3.0 mg) in Freund's complete adjuvant (FAC)."}
{"id": "PMID:1097432", "title": "Binding of aminoacyl-tRNA to ribosomes promoted by elongation factor Tu.  Studies on the role of GTP hydrolysis.", "content": "The role of guanosine triphosphate (GTP) in the elongation factor Tu(EF-Tu)promoted binding of aminoacyl-tRNA to ribosomes has been investigated.  In the presence of EF-Tu and GTP, phenylalanyl-tRNA (Phe-tRNA) was bound to the A site of the poly(U).ribosome complex having N-acetylphenylalanyl-tRNA on its P site.  EF-Tu could be utilized repeatedly in this binding reaction in the presence of GTP but was used only once and remained bound to ribosomes when GTP was replaced by 5'guanylyl methylenediphosphonate (Gpp(CH2)p), a nonhydrolyzable analog of GTP.  The binylalanyl-tRNA, while little dipeptide was formed in the latter case.  However, when EF-Tu and Gpp(CH2)p bound to the ribosomal complex were released by centrifugation through 10% sucrose containing 0.2 mM GDP, the yield of the dipeptide was correspondingly increased.  It was concluded that the role of GTP in  this reaction is to facilitate the transfer of Phe-tRNA to ribosomes by virtue of the high affinity of EF-Tu.GTP for ribosomes.  The subsequent conversion of EF-Tu.GTP to EF-Tu.GDP, a form of EF-Tu with low affinity for ribosomes as well as for Phe-tRNA, resulted in the detachment of EF-Tu.  Thus, the hydrolysis of GTP seems to be required for the release of EF-Tu from ribosomes, which is necessary for peptidyl transfer and the reutilization of EF-Tu.  A freely reversible interaction between ribosome and Phe-tRNA.EF-Tu.Gpp(CH2)p complex was also demonstrated.", "contents": "Binding of aminoacyl-tRNA to ribosomes promoted by elongation factor Tu.  Studies on the role of GTP hydrolysis. The role of guanosine triphosphate (GTP) in the elongation factor Tu(EF-Tu)promoted binding of aminoacyl-tRNA to ribosomes has been investigated.  In the presence of EF-Tu and GTP, phenylalanyl-tRNA (Phe-tRNA) was bound to the A site of the poly(U).ribosome complex having N-acetylphenylalanyl-tRNA on its P site.  EF-Tu could be utilized repeatedly in this binding reaction in the presence of GTP but was used only once and remained bound to ribosomes when GTP was replaced by 5'guanylyl methylenediphosphonate (Gpp(CH2)p), a nonhydrolyzable analog of GTP.  The binylalanyl-tRNA, while little dipeptide was formed in the latter case.  However, when EF-Tu and Gpp(CH2)p bound to the ribosomal complex were released by centrifugation through 10% sucrose containing 0.2 mM GDP, the yield of the dipeptide was correspondingly increased.  It was concluded that the role of GTP in  this reaction is to facilitate the transfer of Phe-tRNA to ribosomes by virtue of the high affinity of EF-Tu.GTP for ribosomes.  The subsequent conversion of EF-Tu.GTP to EF-Tu.GDP, a form of EF-Tu with low affinity for ribosomes as well as for Phe-tRNA, resulted in the detachment of EF-Tu.  Thus, the hydrolysis of GTP seems to be required for the release of EF-Tu from ribosomes, which is necessary for peptidyl transfer and the reutilization of EF-Tu.  A freely reversible interaction between ribosome and Phe-tRNA.EF-Tu.Gpp(CH2)p complex was also demonstrated."}
{"id": "PMID:1097433", "title": "Effects on ribosomes of the substitution of spermidine or divalent cations for magnesium ions.", "content": "The inactivation of rat liver ribosomes (polysomes) resulting from the replacement of bound magnesium by spermidine has been studied using a dialysis method. Inactivation of the ribosomes (polysomes) occurred when more than 40% of the Mg2+ originally bound to ribosomes in the presence of 2 mM Mg2+ was replaced by spermidine. The polypeptide-synthesizing activity of polysomes was retained partially when Mg2+ was replaced by Mn2+ by dialysis. E. coli 30S ribosomal subunits were more resistant to inactivation due to the replacement of Mg2+ by spermidine than the 50S subunits.", "contents": "Effects on ribosomes of the substitution of spermidine or divalent cations for magnesium ions. The inactivation of rat liver ribosomes (polysomes) resulting from the replacement of bound magnesium by spermidine has been studied using a dialysis method. Inactivation of the ribosomes (polysomes) occurred when more than 40% of the Mg2+ originally bound to ribosomes in the presence of 2 mM Mg2+ was replaced by spermidine. The polypeptide-synthesizing activity of polysomes was retained partially when Mg2+ was replaced by Mn2+ by dialysis. E. coli 30S ribosomal subunits were more resistant to inactivation due to the replacement of Mg2+ by spermidine than the 50S subunits."}
{"id": "PMID:1097434", "title": "The involvement of guanosine 5-diphosphate-3-diphosphate in the regulation of phospholipid biosynthesis in Escherichia coli. Lack of ppGpp inhibition of acyltransfer from acyl-ACP to sn-glycerol 3-phosphate.", "content": "The response of the Escherichia coli sn-glycerol-3-phosphate acyltransferase to guanosine 5-diphosphate-3-diphosphate (ppGpp) has been determined in vitro employing palmityl coenzyme A (CoA) and palmityl acyl carrier protein as acyl substrates. Levels of ppGpp which cause significant inhibition of enzyme activity with palmityl-CoA as substrate have no effect on enzyme activity when palmityl acyl carrier protein is employed as acyl donor. The inhibition of enzyme activity observed with palmityl-CoA as acyl substrate was dependent upon the relative concentrations of MgCl2 and ppGpp (MgCl2 to ppGpp ratio) employed. With palmityl-CoA as acyl donor, PPGpp inhibited the production of lysophosphatidic acid but not phosphatidic acid. With palmityl acyl carrier protein as acyl substrate, ppGpp had no influence upon the distribution of the reaction products.", "contents": "The involvement of guanosine 5-diphosphate-3-diphosphate in the regulation of phospholipid biosynthesis in Escherichia coli. Lack of ppGpp inhibition of acyltransfer from acyl-ACP to sn-glycerol 3-phosphate. The response of the Escherichia coli sn-glycerol-3-phosphate acyltransferase to guanosine 5-diphosphate-3-diphosphate (ppGpp) has been determined in vitro employing palmityl coenzyme A (CoA) and palmityl acyl carrier protein as acyl substrates. Levels of ppGpp which cause significant inhibition of enzyme activity with palmityl-CoA as substrate have no effect on enzyme activity when palmityl acyl carrier protein is employed as acyl donor. The inhibition of enzyme activity observed with palmityl-CoA as acyl substrate was dependent upon the relative concentrations of MgCl2 and ppGpp (MgCl2 to ppGpp ratio) employed. With palmityl-CoA as acyl donor, PPGpp inhibited the production of lysophosphatidic acid but not phosphatidic acid. With palmityl acyl carrier protein as acyl substrate, ppGpp had no influence upon the distribution of the reaction products."}
{"id": "PMID:1097435", "title": "The structure of the mutant dihydrofolate reductase from Streptococcus faecium. Amino acid sequence of peptide CNBr 7 and complete sequence of the protein.", "content": "The complete amino acid sequence of the mutant dihydrofolate reductase from Streptococcus faecium var. Durans strain A has been determined by sequence analysis of peptides produced by tryptic, chymotryptic, thermolytic, and mild acid cleavage of the large peptide CNBr 7 and from previously reported studies. The sequence of the S. faecium enzyme is compared to the reported sequence of dihydrofolate reductase from Escherichia coli and the two are shown to contain two domains of substantial homology. One of these domains consists of the NH2-terminal 60 residues and is considered to contribute the dihydrofolate binding site. The second domain probably contains the dinucleotide binding structure. Comparison of the sequences of the dihydrofolate reductases with those of larger dehydrogenases of known structure failed to show any evidence for homology. Considerations of size and predictions of secondary structure also suggest that the second domain in the reductases has no structural similarity to the nucleotide binding site in the larger dehydrogenases. It is concluded that the two reductases are related, although distantly, but that they have evolved from an ancestral protein different from the primitive predecessor of the other oxidoreductases.", "contents": "The structure of the mutant dihydrofolate reductase from Streptococcus faecium. Amino acid sequence of peptide CNBr 7 and complete sequence of the protein. The complete amino acid sequence of the mutant dihydrofolate reductase from Streptococcus faecium var. Durans strain A has been determined by sequence analysis of peptides produced by tryptic, chymotryptic, thermolytic, and mild acid cleavage of the large peptide CNBr 7 and from previously reported studies. The sequence of the S. faecium enzyme is compared to the reported sequence of dihydrofolate reductase from Escherichia coli and the two are shown to contain two domains of substantial homology. One of these domains consists of the NH2-terminal 60 residues and is considered to contribute the dihydrofolate binding site. The second domain probably contains the dinucleotide binding structure. Comparison of the sequences of the dihydrofolate reductases with those of larger dehydrogenases of known structure failed to show any evidence for homology. Considerations of size and predictions of secondary structure also suggest that the second domain in the reductases has no structural similarity to the nucleotide binding site in the larger dehydrogenases. It is concluded that the two reductases are related, although distantly, but that they have evolved from an ancestral protein different from the primitive predecessor of the other oxidoreductases."}
{"id": "PMID:1097436", "title": "Primary structure of porcine pepsin. I. Purification and placement of cyanogen bromide fragments and the amino acid sequence of fragment CB5.", "content": "Fragments resulting from the cyanogen bromide cleavage of reduced and aminoethylated porcine pepsin were purified. Only four of the five fragments theoretically present could be accounted for in major yield when the cyanogen bromide reaction was carried out at room temperature. The NH2-terminal fragment, CB2, contained an internal homoserine which was not cleaved to any significant extent. The amino acid sequence around this internal homoserine was determined by isolating and partially determining the sequence of an alpha-chymotryptic peptide. Cleavage at this methionine was increased by 50% when the cyanogen bromide reaction was carried out at 37 degrees. The NH2- and COOH-terminal sequences of five major fragments were determined. The placement of these fragments in the native pepsin molecule was demonstrated. The amino acid sequence of one of the fragments, CB5, was determined. This fragment contains 44 residues with an internal disulfide bridge. The COOH-terminal methionine of this fragment was connected to another 37-residue cyanogen bromide fragment of known sequence. Together these two fragments formed the COOH-terminal 81 residues of porcine pepsin.", "contents": "Primary structure of porcine pepsin. I. Purification and placement of cyanogen bromide fragments and the amino acid sequence of fragment CB5. Fragments resulting from the cyanogen bromide cleavage of reduced and aminoethylated porcine pepsin were purified. Only four of the five fragments theoretically present could be accounted for in major yield when the cyanogen bromide reaction was carried out at room temperature. The NH2-terminal fragment, CB2, contained an internal homoserine which was not cleaved to any significant extent. The amino acid sequence around this internal homoserine was determined by isolating and partially determining the sequence of an alpha-chymotryptic peptide. Cleavage at this methionine was increased by 50% when the cyanogen bromide reaction was carried out at 37 degrees. The NH2- and COOH-terminal sequences of five major fragments were determined. The placement of these fragments in the native pepsin molecule was demonstrated. The amino acid sequence of one of the fragments, CB5, was determined. This fragment contains 44 residues with an internal disulfide bridge. The COOH-terminal methionine of this fragment was connected to another 37-residue cyanogen bromide fragment of known sequence. Together these two fragments formed the COOH-terminal 81 residues of porcine pepsin."}
{"id": "PMID:1097437", "title": "Primary structure of porcine pepsin. II. Amino acid sequence of two cyanogen bromide fragments, CB3 and CB4.", "content": "The amino acid sequences of two cyanogen bromide fragments from porcine pepsin have been determined. Fragment CB3 which represents the NH2-terminal 80 residues of pepsin was assembled from the peptides purified from proteolytic digests of this fragment using alpha-chymotrypsin, thermolysin, and staphylococcal protease. Two chymotryptic peptides were isolated from the NH2-terminal region of this fragment. One of these contains 2 extra residues, Ala-Leu-, at the NH2 terminus. This peptide is apparently derived from a different cleavage site of pepsinogen in its conversion to pepsin. The second cyanogen bromide fragment, CB4, contains 47 residues. The sequence was established from the peptides resulting from proteolytic digests using alpha-chymotrypsin, alpha-lytic protease, and thermolysin. An isoleucyl residue at position 29 of fragment CB4 appears to be absent in some molecules. This represents a structural variant of pepsin.", "contents": "Primary structure of porcine pepsin. II. Amino acid sequence of two cyanogen bromide fragments, CB3 and CB4. The amino acid sequences of two cyanogen bromide fragments from porcine pepsin have been determined. Fragment CB3 which represents the NH2-terminal 80 residues of pepsin was assembled from the peptides purified from proteolytic digests of this fragment using alpha-chymotrypsin, thermolysin, and staphylococcal protease. Two chymotryptic peptides were isolated from the NH2-terminal region of this fragment. One of these contains 2 extra residues, Ala-Leu-, at the NH2 terminus. This peptide is apparently derived from a different cleavage site of pepsinogen in its conversion to pepsin. The second cyanogen bromide fragment, CB4, contains 47 residues. The sequence was established from the peptides resulting from proteolytic digests using alpha-chymotrypsin, alpha-lytic protease, and thermolysin. An isoleucyl residue at position 29 of fragment CB4 appears to be absent in some molecules. This represents a structural variant of pepsin."}
{"id": "PMID:1097438", "title": "Primary structure of porcine pepsin. III. Amino acid sequence of a cyanogen bromide fragment, CB2A, and the complete structure of porcine pepsin.", "content": "The complete amino acid sequence of porcine pepsin (EC 3.4.4.1) was constructed from the sequence of five cyanogen bromide fragments. The sequence of one of these fragments, CB2A, is reported here. The sequences of 4 other fragments are known from previous work. Porcine pepsin contains 327 residues with three structural variants. The active center aspartyl residue, which reacts with 1,2-epoxy-3-(p-nitrophenoxy)propane (Chen, K. C. S., and Tang, J. (1972) J. Biol. Chem. 247, 2566-2574), is located at residue 32. Another active site aspartyl residue, which reacts with diazo inactivators (Bayliss, R. S., Knowles, J. B., and Wybrandt, G. B. (1969) Biochem. J. 113, 377-386, IS LOCATED AT RESIDUE 215. The sequences around these 2 aspartyl residues are apparently homologous to each other. The sequences around the tryptophanyl residues at positions 39, 141, 181, and 300 are also homologous to one another. These homologous sequences could be genetic in origin. Fragment CB2A which contains 119 residues was constructed from the peptide sequences resulting from six proteolytic digestions and chemical cleavage at tryptophanyl bonds.", "contents": "Primary structure of porcine pepsin. III. Amino acid sequence of a cyanogen bromide fragment, CB2A, and the complete structure of porcine pepsin. The complete amino acid sequence of porcine pepsin (EC 3.4.4.1) was constructed from the sequence of five cyanogen bromide fragments. The sequence of one of these fragments, CB2A, is reported here. The sequences of 4 other fragments are known from previous work. Porcine pepsin contains 327 residues with three structural variants. The active center aspartyl residue, which reacts with 1,2-epoxy-3-(p-nitrophenoxy)propane (Chen, K. C. S., and Tang, J. (1972) J. Biol. Chem. 247, 2566-2574), is located at residue 32. Another active site aspartyl residue, which reacts with diazo inactivators (Bayliss, R. S., Knowles, J. B., and Wybrandt, G. B. (1969) Biochem. J. 113, 377-386, IS LOCATED AT RESIDUE 215. The sequences around these 2 aspartyl residues are apparently homologous to each other. The sequences around the tryptophanyl residues at positions 39, 141, 181, and 300 are also homologous to one another. These homologous sequences could be genetic in origin. Fragment CB2A which contains 119 residues was constructed from the peptide sequences resulting from six proteolytic digestions and chemical cleavage at tryptophanyl bonds."}
{"id": "PMID:1097439", "title": "Effects of estrogen on gene expression in the chick oviduct. IV. Initiation of RNA synthesis on DNA and chromatin.", "content": "The interaction of Escherichia coli RNA polymerase (ribonucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6.) with chick oviduct DNA and chromatin has been studied in order to quantify the RNA polymerase binding sites and rifampicin-resistant initiation sites. The number of high affinity binding sites at saturation was calculated from the number of molecules of enzyme bound to DNA or to chromatin. The number of rifampicin-resistant initiation sites was calculated from the total quantity and the number average chain length of RNA synthesized under the conditions in which only RNA polymerase already in a stable preinitiation complex could avoid inhibition by the drug. Using an assessment of the temperature requirement for the information of this preinitiation complex, we were able to differentiate initiations at single-stranded or nicked DNA regions from initiations at double-stranded DNA regions. This method enabled us to rule out the possibility that RNA chain initiation on chromatin was due to initiation at nicked or end regions of DNA. In addition, we also demonstrated that not all RNA polymerase molecules bound to DNA or chromatin are located at a site at which RNA chain initiation can immediately begin. This methodological approach should allow investigators to monitor the individual reactions and factors involved in in vitro transcription of eukaryotic DNA and chromatin.", "contents": "Effects of estrogen on gene expression in the chick oviduct. IV. Initiation of RNA synthesis on DNA and chromatin. The interaction of Escherichia coli RNA polymerase (ribonucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6.) with chick oviduct DNA and chromatin has been studied in order to quantify the RNA polymerase binding sites and rifampicin-resistant initiation sites. The number of high affinity binding sites at saturation was calculated from the number of molecules of enzyme bound to DNA or to chromatin. The number of rifampicin-resistant initiation sites was calculated from the total quantity and the number average chain length of RNA synthesized under the conditions in which only RNA polymerase already in a stable preinitiation complex could avoid inhibition by the drug. Using an assessment of the temperature requirement for the information of this preinitiation complex, we were able to differentiate initiations at single-stranded or nicked DNA regions from initiations at double-stranded DNA regions. This method enabled us to rule out the possibility that RNA chain initiation on chromatin was due to initiation at nicked or end regions of DNA. In addition, we also demonstrated that not all RNA polymerase molecules bound to DNA or chromatin are located at a site at which RNA chain initiation can immediately begin. This methodological approach should allow investigators to monitor the individual reactions and factors involved in in vitro transcription of eukaryotic DNA and chromatin."}
{"id": "PMID:1097440", "title": "Effect of estrogen on gene expression in the chick oviduct. V. Changes in the number of RNA polymerase binding and initiation sites in chromatin.", "content": "Estrogen administration to chicks results in an increase in the chromatin template activity of oviduct target tissue as assayed under standard in vitro assay conditions. However, the results obtained by the simple measurement of template activity may be a complicated function of the number of available RNA polymerase initiation sites, the rate of RNA chain elongation, and the rate of reinitiation. In the present study, we have measured separately the change in both the number of chain initiations as well as the rate of RNA chain propagation under conditions in which reinitiation was eliminated. Chromatin prepared from either estrogen-treated or control oviducts both supported an RNA chain elongation rate of six nucleotides per s and a chain size of approximately 700 nucleotides. Thus, both the elongation rate and size of the average product remained relatively constant following estrogen stimulation. In contrast, within 8 hours after a single injection of estrogen to unstimulated chicks, the concentration of RNA polymerase needed to saturate chromatin binding sites was increased to 150% in comparison to control values, and by 24 hours the level of polymerase bound to chromatin was twice that of the untreated control chick chromatin. With daily injections of estrogen, polymerase binding continued to rise. Coincident with the over-all increase in chromatin-bound polymerase was an increase in rifampicin-insensitive initiation sites and newly synthesized RNA chains. Unstimulated chick oviduct chromatin initiated 10,000 RNA chains/pg of DNA, while 24 hours of steroid treatment increased the number of initiated chains to 34,000 chains. These data demonstrate that the estrogen-induced increase in chromatin transcriptive activity was due to an increased number of polymerase binding and initiation sites on the chromatin template without a detectable change in the rate of RNA chain elongation.", "contents": "Effect of estrogen on gene expression in the chick oviduct. V. Changes in the number of RNA polymerase binding and initiation sites in chromatin. Estrogen administration to chicks results in an increase in the chromatin template activity of oviduct target tissue as assayed under standard in vitro assay conditions. However, the results obtained by the simple measurement of template activity may be a complicated function of the number of available RNA polymerase initiation sites, the rate of RNA chain elongation, and the rate of reinitiation. In the present study, we have measured separately the change in both the number of chain initiations as well as the rate of RNA chain propagation under conditions in which reinitiation was eliminated. Chromatin prepared from either estrogen-treated or control oviducts both supported an RNA chain elongation rate of six nucleotides per s and a chain size of approximately 700 nucleotides. Thus, both the elongation rate and size of the average product remained relatively constant following estrogen stimulation. In contrast, within 8 hours after a single injection of estrogen to unstimulated chicks, the concentration of RNA polymerase needed to saturate chromatin binding sites was increased to 150% in comparison to control values, and by 24 hours the level of polymerase bound to chromatin was twice that of the untreated control chick chromatin. With daily injections of estrogen, polymerase binding continued to rise. Coincident with the over-all increase in chromatin-bound polymerase was an increase in rifampicin-insensitive initiation sites and newly synthesized RNA chains. Unstimulated chick oviduct chromatin initiated 10,000 RNA chains/pg of DNA, while 24 hours of steroid treatment increased the number of initiated chains to 34,000 chains. These data demonstrate that the estrogen-induced increase in chromatin transcriptive activity was due to an increased number of polymerase binding and initiation sites on the chromatin template without a detectable change in the rate of RNA chain elongation."}
{"id": "PMID:1097441", "title": "The amino acid sequence of the insulin from a primitive vertebrate, the atlantic hagfish (Myxine glutinosa).", "content": "Insulin has been isolated and purified from the islet organs of the cyclostome, Myxine glutinosa, by means of acid-ethanol extraction, fractional precipitation, and gel filtration. The complete amino acid sequence of the hormone has been determined by Edman degradation of the S-carboxymethylated or performic acid-oxidized A and B chains, and of various tryptic peptides derived from the chains. The 52-residue hagfish insulin has many structural features in common with other vertebrate insulins including the locations of the 6 half-cystine residues, the NH2-terminal 7 residues and the COOH-terminal 6 residues of the A chain, and several shorter sequences in the B chain that are known to comprise the dimer interface in porcine insulin crystals. Of the 24 residues which are invariant among the other known insulins, 23 are identical in hagfish int 16 of these sites it contains residues not previously observed in vertebrate insulins. The B chain also contains an additional COOH-terminal residue of methionine, making it 1 residue longer than the usual 30-residue mammalian B chains. Several features of the tertiary and quaternary structure of hagfish insulin, including the probable absence of a metal ion-stabilized hexameric form, are discussed on the basis of these findings. The results suggest that the conformation of the insulin molecule has been well conserved throughout the entire evolution of the vertebrates.", "contents": "The amino acid sequence of the insulin from a primitive vertebrate, the atlantic hagfish (Myxine glutinosa). Insulin has been isolated and purified from the islet organs of the cyclostome, Myxine glutinosa, by means of acid-ethanol extraction, fractional precipitation, and gel filtration. The complete amino acid sequence of the hormone has been determined by Edman degradation of the S-carboxymethylated or performic acid-oxidized A and B chains, and of various tryptic peptides derived from the chains. The 52-residue hagfish insulin has many structural features in common with other vertebrate insulins including the locations of the 6 half-cystine residues, the NH2-terminal 7 residues and the COOH-terminal 6 residues of the A chain, and several shorter sequences in the B chain that are known to comprise the dimer interface in porcine insulin crystals. Of the 24 residues which are invariant among the other known insulins, 23 are identical in hagfish int 16 of these sites it contains residues not previously observed in vertebrate insulins. The B chain also contains an additional COOH-terminal residue of methionine, making it 1 residue longer than the usual 30-residue mammalian B chains. Several features of the tertiary and quaternary structure of hagfish insulin, including the probable absence of a metal ion-stabilized hexameric form, are discussed on the basis of these findings. The results suggest that the conformation of the insulin molecule has been well conserved throughout the entire evolution of the vertebrates."}
{"id": "PMID:1097442", "title": "Initiation of protein synthesis. Binding of messenger RNA.", "content": "Complexes between 30 S ribosomal subunits and fMet-tRNA are formed during incubation of 30 S subunits with fMet-tRNA and all other components for initiation of protein synthesis, except R17 bacteriophage RNA. That these complexes serve as intermediates in the binding of messenger RNA is demonstrated directly by the finding that upon addition of R17 RNA, fMet-tRNA in preformed fMet-RNA-30 S complexes preferentially enters fMet-tRNA-30 S-R17 RNA complexes. On the other hand, incubation of 30 S ribosomal subunits with R17 RNA and all other components for initiation except fMet-tRNA does not yield 30 S-R17 RNA complexes that can act subsequently as functional intermediates in the binding of fMet-tRNA: formation of fMet-tRNA-30 S-R17 RNA complexes does not occur when fMet-tRNA is added and further binding of R17 RNA to 30 S subunits is prevented by specific inhibitors. These experiments lead to an unambiguous order of events in the sequence of initiation, in which binding of fMet-tRNA to the small ribosomal subunit must occur before messenger RNA can be bound and phased correctly. Complexes between fMet-tRNA and 60 S subunits are in rapid equilibrium with the free components, and have a half-life of less than 2 min at 37 degrees. This explains why such complexes are not detected in sucrose gradients, unless they are first fixed with glutaraldehyde. Attachment of R17 RNA, however, results in formation of an fMet-tRNA-30 S-R17 RNA complex that is stabilized greatly; fMet-tRNA in this complex exchanges only very slowly with free fMet-tRNA. Initiation factor IF-3 has two functions in initiation. The first is to direct the binding of messenger RNA to the 30 S-fMet-tRNA complex. This function is not needed when initiation complex formation occurs on ApUpG triplets, in which case the second function of IF-3 is detected, that of providing free 30 S subunits for initiation. The ability of IF-3 to bind directly to R17 RNA may be related to its requirement in messenger RNA recognition. However, since IF-3 exhibits a greater affinity for the 30 S subunit than for R17 RNA, it appears that the recognition function of IF-3 is expressed while IF-3 is associated with the 30 S subunit.", "contents": "Initiation of protein synthesis. Binding of messenger RNA. Complexes between 30 S ribosomal subunits and fMet-tRNA are formed during incubation of 30 S subunits with fMet-tRNA and all other components for initiation of protein synthesis, except R17 bacteriophage RNA. That these complexes serve as intermediates in the binding of messenger RNA is demonstrated directly by the finding that upon addition of R17 RNA, fMet-tRNA in preformed fMet-RNA-30 S complexes preferentially enters fMet-tRNA-30 S-R17 RNA complexes. On the other hand, incubation of 30 S ribosomal subunits with R17 RNA and all other components for initiation except fMet-tRNA does not yield 30 S-R17 RNA complexes that can act subsequently as functional intermediates in the binding of fMet-tRNA: formation of fMet-tRNA-30 S-R17 RNA complexes does not occur when fMet-tRNA is added and further binding of R17 RNA to 30 S subunits is prevented by specific inhibitors. These experiments lead to an unambiguous order of events in the sequence of initiation, in which binding of fMet-tRNA to the small ribosomal subunit must occur before messenger RNA can be bound and phased correctly. Complexes between fMet-tRNA and 60 S subunits are in rapid equilibrium with the free components, and have a half-life of less than 2 min at 37 degrees. This explains why such complexes are not detected in sucrose gradients, unless they are first fixed with glutaraldehyde. Attachment of R17 RNA, however, results in formation of an fMet-tRNA-30 S-R17 RNA complex that is stabilized greatly; fMet-tRNA in this complex exchanges only very slowly with free fMet-tRNA. Initiation factor IF-3 has two functions in initiation. The first is to direct the binding of messenger RNA to the 30 S-fMet-tRNA complex. This function is not needed when initiation complex formation occurs on ApUpG triplets, in which case the second function of IF-3 is detected, that of providing free 30 S subunits for initiation. The ability of IF-3 to bind directly to R17 RNA may be related to its requirement in messenger RNA recognition. However, since IF-3 exhibits a greater affinity for the 30 S subunit than for R17 RNA, it appears that the recognition function of IF-3 is expressed while IF-3 is associated with the 30 S subunit."}
{"id": "PMID:1097443", "title": "Translational repression of a viral messenger RNA by a host protein.", "content": "It is shown that factor i, a bacterial protein, specifically inhibits that step in the initiation of R17 bacteriophage RNA translation that involves the attachment of native R17 RNA to 30 S ribosomal subunits carrying fMet-tRNA. This inhibition by factor i is relieved by the addition of excess R17 RNA, but not by the addition of excess 30 S subunits. That R17 RNA is the only target of the inhibition is demonstrated further by the fact that in a cell-free extract containing all components for protein synthesis, factor i-mediated inhibition of exogenous R17 RNA translation can be overcome only by the addition of excess R17 RNA and not by excess cell-free extract. Upon relief of inhibition, phage coat protein synthesis is restored; enhancement of formation of other cistron products is not seen. While initiation of R17 RNA translation is blocked by factor i, chain elongation is not affected. Although foactor i inhibits the IF-3-dependent binding of R17 RNA to fMet-tRNA-30 S complexes, under conditions of initiation of protein synthesis formation of stable complexes between factor i and IF-3 could not be detected, and factor i did not interfere with the binding of IF-3 to free, native R17 RNA. Instead of affecting the function of IF-3 or ribosomes, factor i exerts its inhibition by binding to R17 RNA and acting as a translational repressor. Factor i prefers intact R17 RNA to fragments generated by autoradiolysis; its binding to R17 RNA is specific in that little competition is observed by transfer RNA, ribosomal RNA or poly(A). However, factor i has a high affinity for poly(U) sequences.", "contents": "Translational repression of a viral messenger RNA by a host protein. It is shown that factor i, a bacterial protein, specifically inhibits that step in the initiation of R17 bacteriophage RNA translation that involves the attachment of native R17 RNA to 30 S ribosomal subunits carrying fMet-tRNA. This inhibition by factor i is relieved by the addition of excess R17 RNA, but not by the addition of excess 30 S subunits. That R17 RNA is the only target of the inhibition is demonstrated further by the fact that in a cell-free extract containing all components for protein synthesis, factor i-mediated inhibition of exogenous R17 RNA translation can be overcome only by the addition of excess R17 RNA and not by excess cell-free extract. Upon relief of inhibition, phage coat protein synthesis is restored; enhancement of formation of other cistron products is not seen. While initiation of R17 RNA translation is blocked by factor i, chain elongation is not affected. Although foactor i inhibits the IF-3-dependent binding of R17 RNA to fMet-tRNA-30 S complexes, under conditions of initiation of protein synthesis formation of stable complexes between factor i and IF-3 could not be detected, and factor i did not interfere with the binding of IF-3 to free, native R17 RNA. Instead of affecting the function of IF-3 or ribosomes, factor i exerts its inhibition by binding to R17 RNA and acting as a translational repressor. Factor i prefers intact R17 RNA to fragments generated by autoradiolysis; its binding to R17 RNA is specific in that little competition is observed by transfer RNA, ribosomal RNA or poly(A). However, factor i has a high affinity for poly(U) sequences."}
{"id": "PMID:1097444", "title": "Regulation of membrane lipid synthesis in Escherichia coli. Accumulation of free fatty acids of abnormal length during inhibition of phospholipid synthesis.", "content": "Glycerol starvation of an Escherichia coli glycerol auxotroph results in a specific inhibition of membrane phospholipid synthesis. Mindich ((1972) J. Bacteriol. 110, 96-102) observed only a trace accumulation of free fatty acid following glycerol deprivation. We have repeated these experiments using glycerol auxotrophs which also possess a lesion in beta oxidation. This defect was introduced in order to control fatty acid degradation. In contrast to the previous results, we find free fatty acid does accumulate during glycerol starvation. Similar results were found using beta oxidation-defective (fadE-) derivatives of both gpsA and plsB glycerol auxotrophs. Upon glycerol starvation of a plsB- fadE- strain, phospholipid synthesis is 90 percent inhibited. Following a lag of 20 to 40 min, free fatty acid synthesis begins and proceeds at a rate that steadily increases until the rate of fatty acid synthesis is equal to that found in glycerol-supplemented cultures. The accumulation of free fatty acid is the result of de novo synthesis.  The average chain length of the fatty acid in the unesterified fraction is abnormally long. Two 20-carbon fatty acids, cis-13-eicosenoic acid and arachidic acid, are found in this frction. Furthermore, a greatly increased level of stearic acid and a small amount of a C-22 (behenic) acid are found in the free fatty acid fraction. These data indicate that acyl transfer into phospholipid is a major determinant of phospholipid acyl moiety chain length. Other experiments have shown that the free fatty acid fraction in glycerol-starved cells is metabolically active. This fraction turns over despite the defective beta oxidation system. Restoration of glycerol to starved cells allows the incorporation of the unesterified fatty acids into phospholipid.", "contents": "Regulation of membrane lipid synthesis in Escherichia coli. Accumulation of free fatty acids of abnormal length during inhibition of phospholipid synthesis. Glycerol starvation of an Escherichia coli glycerol auxotroph results in a specific inhibition of membrane phospholipid synthesis. Mindich ((1972) J. Bacteriol. 110, 96-102) observed only a trace accumulation of free fatty acid following glycerol deprivation. We have repeated these experiments using glycerol auxotrophs which also possess a lesion in beta oxidation. This defect was introduced in order to control fatty acid degradation. In contrast to the previous results, we find free fatty acid does accumulate during glycerol starvation. Similar results were found using beta oxidation-defective (fadE-) derivatives of both gpsA and plsB glycerol auxotrophs. Upon glycerol starvation of a plsB- fadE- strain, phospholipid synthesis is 90 percent inhibited. Following a lag of 20 to 40 min, free fatty acid synthesis begins and proceeds at a rate that steadily increases until the rate of fatty acid synthesis is equal to that found in glycerol-supplemented cultures. The accumulation of free fatty acid is the result of de novo synthesis.  The average chain length of the fatty acid in the unesterified fraction is abnormally long. Two 20-carbon fatty acids, cis-13-eicosenoic acid and arachidic acid, are found in this frction. Furthermore, a greatly increased level of stearic acid and a small amount of a C-22 (behenic) acid are found in the free fatty acid fraction. These data indicate that acyl transfer into phospholipid is a major determinant of phospholipid acyl moiety chain length. Other experiments have shown that the free fatty acid fraction in glycerol-starved cells is metabolically active. This fraction turns over despite the defective beta oxidation system. Restoration of glycerol to starved cells allows the incorporation of the unesterified fatty acids into phospholipid."}
{"id": "PMID:1097445", "title": "Ten proteins required for conversion of phiX174 single-stranded DNA to duplex form in vitro. Resolution and reconstitution.", "content": "Protein requirements for conversion of phiX174 single-stranded DNA to a double-stranded replicative form with a small gap (RF II) have been determined by resolution and reconstitution of the multienzyme system from extracts of gently lysed Escherichia coli. Assays depended on: (a) complementation of extracts of thermosensitive mutants and (b) fractionation of extracts of wild type cells to divide essential components into groups, each of which was further resolved. These procedures have yielded eight proteins: dnaB protein, dnaC protein, proteins i and n (two novel proteins without a defined genetic locus), dnaG protein, DNA polymerase III holoenzyme (polymerase III and copolymerase III), and DNA unwinding protein; purification procedures for the first four are presented here. (Closure of RF 22 requires as with phage M13, DNA polymerase I and ligase.)", "contents": "Ten proteins required for conversion of phiX174 single-stranded DNA to duplex form in vitro. Resolution and reconstitution. Protein requirements for conversion of phiX174 single-stranded DNA to a double-stranded replicative form with a small gap (RF II) have been determined by resolution and reconstitution of the multienzyme system from extracts of gently lysed Escherichia coli. Assays depended on: (a) complementation of extracts of thermosensitive mutants and (b) fractionation of extracts of wild type cells to divide essential components into groups, each of which was further resolved. These procedures have yielded eight proteins: dnaB protein, dnaC protein, proteins i and n (two novel proteins without a defined genetic locus), dnaG protein, DNA polymerase III holoenzyme (polymerase III and copolymerase III), and DNA unwinding protein; purification procedures for the first four are presented here. (Closure of RF 22 requires as with phage M13, DNA polymerase I and ligase.)"}
{"id": "PMID:1097446", "title": "dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.", "content": "The protein responsible for the initiation of conversion of single-stranded phage G4 DNA to the duplex replicative form has been purified approximately 3000-fold and identified with Escherichia coli dnaG gene product. The protein is a rifampicin-resistant RNA polymerase of approximately 64,000 daltons. It catalyzes the incorporation of the four ribonucleoside triphosphates into an oligoribonucleotide, using as template the single-stranded DNA coated with the DNA unwinding protein of E. coli. An RNA transcript of a unique region of the chromosome can serve as a primer by covalent extension by DNA polymerase III holoenzyme to form a nearly full-length linear complementary strand. A similar role for the dnaG protein in the initiation of nascent (Okazaki) fragments in replication of the host chromosome is discussed.", "contents": "dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form. The protein responsible for the initiation of conversion of single-stranded phage G4 DNA to the duplex replicative form has been purified approximately 3000-fold and identified with Escherichia coli dnaG gene product. The protein is a rifampicin-resistant RNA polymerase of approximately 64,000 daltons. It catalyzes the incorporation of the four ribonucleoside triphosphates into an oligoribonucleotide, using as template the single-stranded DNA coated with the DNA unwinding protein of E. coli. An RNA transcript of a unique region of the chromosome can serve as a primer by covalent extension by DNA polymerase III holoenzyme to form a nearly full-length linear complementary strand. A similar role for the dnaG protein in the initiation of nascent (Okazaki) fragments in replication of the host chromosome is discussed."}
{"id": "PMID:1097448", "title": "Myelodysplastics--fate of those followed for twenty years or more.", "content": "Of a total of 143 myelodysplastic patients treated between 1928 and 1951, there were sixty-three patients with severe myelodysplasia whose records allowed long-term review. At the time of writing twenty-nine were alive and were twenty to forty-three years old. All were walking in the hospital while under an intensive physical therapy program. However only two of the nine with twelfth thoracic-second lumbar function were walking at final follow-up as adults, while nineteen of the twenty with function at the third lumbar level were doing so. The status of the hips did not correlate with the ability to walk. One-third of the survivors were self-supporting at the time of writing. About one-half had scoliosis and in one-third was greater than 20 degrees.", "contents": "Myelodysplastics--fate of those followed for twenty years or more. Of a total of 143 myelodysplastic patients treated between 1928 and 1951, there were sixty-three patients with severe myelodysplasia whose records allowed long-term review. At the time of writing twenty-nine were alive and were twenty to forty-three years old. All were walking in the hospital while under an intensive physical therapy program. However only two of the nine with twelfth thoracic-second lumbar function were walking at final follow-up as adults, while nineteen of the twenty with function at the third lumbar level were doing so. The status of the hips did not correlate with the ability to walk. One-third of the survivors were self-supporting at the time of writing. About one-half had scoliosis and in one-third was greater than 20 degrees."}
{"id": "PMID:1097454", "title": "The use of flow microbluorimetry in the analysis of the phenotype expression of mouse histocompatibility antigens.", "content": "Quantitation of the expression of cell surface antigens has hitherto been limited to analysis by either cytotoxicity tests or radioimmune assays (5, 15). We report here the use of a new methodology to analyze and quantitate the expression of mouse histocompabililty antigens (H-2 locus) in hybrid clones and parental cell types. The binding of fluorescein-tagged antibody is measured on a cell-to-cell basis in large viable cell populations using flow microfluorimetric techniques. These techniques have been used to measure hapten and immunoglobulin binding to lymphocyte populations (8, 9, 14). However, this is the first report in which these techniques have been used to examine the expression of the H-2 locus. The advantage of this approach is twofold: first, a large and statistically significant sample population may be analyzed one cell at a time, thus revealing the fine detail of heterogeneity in the expression of the cell surface markers within a population. Second, as has been demonstrated for analysis of specific components of the immune system, this method does permit fluorescence-activated sorting of cell types according to their different surface populations (8, 9, 14).", "contents": "The use of flow microbluorimetry in the analysis of the phenotype expression of mouse histocompatibility antigens. Quantitation of the expression of cell surface antigens has hitherto been limited to analysis by either cytotoxicity tests or radioimmune assays (5, 15). We report here the use of a new methodology to analyze and quantitate the expression of mouse histocompabililty antigens (H-2 locus) in hybrid clones and parental cell types. The binding of fluorescein-tagged antibody is measured on a cell-to-cell basis in large viable cell populations using flow microfluorimetric techniques. These techniques have been used to measure hapten and immunoglobulin binding to lymphocyte populations (8, 9, 14). However, this is the first report in which these techniques have been used to examine the expression of the H-2 locus. The advantage of this approach is twofold: first, a large and statistically significant sample population may be analyzed one cell at a time, thus revealing the fine detail of heterogeneity in the expression of the cell surface markers within a population. Second, as has been demonstrated for analysis of specific components of the immune system, this method does permit fluorescence-activated sorting of cell types according to their different surface populations (8, 9, 14)."}
{"id": "PMID:1097455", "title": "Fine structure of an organelle associated with the nucleus and cytoplasmic microtubules in the cellular slime mould Polysphondylium violaceum.", "content": "Polysphondylium violaceum was grown in association with Escherichia coli. Vegetative amoebae and pseudoplasmodia were fixed under different conditions and processed for electron microscopy. An electron-opaque body (nucleus-associated body, NAB) lies in the cytoplasm near the tapered end of interphase nuclei. The NAB consists of a disk-shaped, multilayered core, approximately 200 nm in diameter and 150 nm thick, embedded in a granular matrix from which electron-opaque nodules protrude. The nodules are termination points of microtubules radiating from the NAB into the cytoplasm or running along the nucleus. On the average there are 16 nodules per NAB. One or two microtubules terminate in each nodule. Spindle pole bodies, arising by duplication of the NAB at the beginning of mitosis, are unstructured foci for spindle microtubules in mitotic cells. It is suggested that cytoplasmic microtubules do not determine cell shape, but they probably cause the tapering deformation of the nucleus. They may, furthermore, represent a storage form of subunits for utilization during the formation of the mitotic spindle. The nodules of the NAB are potential nucleation sites of cytoplasmic microtubules during interphase. Spindle pole bodies presumably acquire a microtubule organizing capability by integration of the decondensed nodules.", "contents": "Fine structure of an organelle associated with the nucleus and cytoplasmic microtubules in the cellular slime mould Polysphondylium violaceum. Polysphondylium violaceum was grown in association with Escherichia coli. Vegetative amoebae and pseudoplasmodia were fixed under different conditions and processed for electron microscopy. An electron-opaque body (nucleus-associated body, NAB) lies in the cytoplasm near the tapered end of interphase nuclei. The NAB consists of a disk-shaped, multilayered core, approximately 200 nm in diameter and 150 nm thick, embedded in a granular matrix from which electron-opaque nodules protrude. The nodules are termination points of microtubules radiating from the NAB into the cytoplasm or running along the nucleus. On the average there are 16 nodules per NAB. One or two microtubules terminate in each nodule. Spindle pole bodies, arising by duplication of the NAB at the beginning of mitosis, are unstructured foci for spindle microtubules in mitotic cells. It is suggested that cytoplasmic microtubules do not determine cell shape, but they probably cause the tapering deformation of the nucleus. They may, furthermore, represent a storage form of subunits for utilization during the formation of the mitotic spindle. The nodules of the NAB are potential nucleation sites of cytoplasmic microtubules during interphase. Spindle pole bodies presumably acquire a microtubule organizing capability by integration of the decondensed nodules."}
{"id": "PMID:1097457", "title": "Chromatography of acid proteinases and chymotrypsin on a sorbent containing 2,4-dinitrophenyl residues.", "content": "A sorbent obtained by treatment of cyanogen bromide-activated Sepharose 4B with mono-N-DNP-hexamethylenediamine has been shown to be effective in the affinity chromatography of pepsin, pepsinogen and acid proteinase from Aspergillus awamori. It is considered that 2,4-dinitrophenyl residues of the sorbent interact specifically with the hydrophobic zone of the enzyme, which may belong to the substrate binding site. The chromatography of chymotrypsin on the same sorbent supports this assumption.", "contents": "Chromatography of acid proteinases and chymotrypsin on a sorbent containing 2,4-dinitrophenyl residues. A sorbent obtained by treatment of cyanogen bromide-activated Sepharose 4B with mono-N-DNP-hexamethylenediamine has been shown to be effective in the affinity chromatography of pepsin, pepsinogen and acid proteinase from Aspergillus awamori. It is considered that 2,4-dinitrophenyl residues of the sorbent interact specifically with the hydrophobic zone of the enzyme, which may belong to the substrate binding site. The chromatography of chymotrypsin on the same sorbent supports this assumption."}
{"id": "PMID:1097452", "title": "Tolerance and resistance of myocardium to anoxia: experimental studies.", "content": "We shall emphasize the notion of wide variability in the tolerance and resistance to anoxia of the myocardium, although the most important parameters are temperature and time. They can be modified widely by accompanying physical or biochemical conditions: --Cardiac distension and drugs inducing cardiac arrest; --Resuscitative perfusion; --The effect of some biochemical components is more likely explained by their possible action on the mitochondriae (glyoxilic acid-Isoproterenol). The study of the mitochondriae alone can explain the different behaviours of hearts subjected to anoxia in various situations.", "contents": "Tolerance and resistance of myocardium to anoxia: experimental studies. We shall emphasize the notion of wide variability in the tolerance and resistance to anoxia of the myocardium, although the most important parameters are temperature and time. They can be modified widely by accompanying physical or biochemical conditions: --Cardiac distension and drugs inducing cardiac arrest; --Resuscitative perfusion; --The effect of some biochemical components is more likely explained by their possible action on the mitochondriae (glyoxilic acid-Isoproterenol). The study of the mitochondriae alone can explain the different behaviours of hearts subjected to anoxia in various situations."}
{"id": "PMID:1097453", "title": "Intraoesophageal rupture of a thoracic aortic aneurysm.", "content": "The intraoesophageal rupture of a large thoracic aortic aneurysm is reported in a 49 year old man. He had been hypertensive for some years while the aneurysm increased in size. Although a graft was successfully inserted to repair the leak, infection from the oesophagus with candida albicans, subsequently led to secondary haemorrhage and death 17 days later. A plea is made for the earlier referral of patients with aneurysm prior to rupture, as the operative mortality rises markedly after rupture has occurred and in this case the situation was virtually irreparable.", "contents": "Intraoesophageal rupture of a thoracic aortic aneurysm. The intraoesophageal rupture of a large thoracic aortic aneurysm is reported in a 49 year old man. He had been hypertensive for some years while the aneurysm increased in size. Although a graft was successfully inserted to repair the leak, infection from the oesophagus with candida albicans, subsequently led to secondary haemorrhage and death 17 days later. A plea is made for the earlier referral of patients with aneurysm prior to rupture, as the operative mortality rises markedly after rupture has occurred and in this case the situation was virtually irreparable."}
{"id": "PMID:1097461", "title": "LH and FSH response to gonadotropin-releasing hormone in anorexia nervosa: Effect of nutritional rehabilitation.", "content": "The LH and FSH responses to the administration of synthetic gonadotropin-releasing hormone (GnRH) were assessed in 14 patients with anorexia nervosa before, during and following weight gain. In 8 patients studied prior to therapy when they were at 53-64% of idal body weight, the LH response to GnRH was strikingly impaired while the FSH response was equal to that observed in normally menstruating women. After return to 79-88% of ideal body weight the LH response was improved but remained less than in the normal women. In a second group of 6 patients who were studied 4-11 months after hospital discharge when they were 90-94% of ideal body weight, the LH response was always equal to or greater than that found in the normal women. The serum estradiol concentration was low in all of the patients and remained less than 33 pg/ml even in those who returned to more than 90% of ideal body weight. Treatment with 25 mug of ethinyl estradiol for 3 days prior to repeat administration of GnRH did not result in a statistically significant increase of the LH or FSH responses above those before treatment. These data suggest changes occur in both hypothalamic and pituitary regulation of gonadotropin secretion in the course of nutritional rehabilitation of patients with anorexia nervosa and that synthesis and secretion of LH and FSH are modulated independently at the level of the pituitary gland.", "contents": "LH and FSH response to gonadotropin-releasing hormone in anorexia nervosa: Effect of nutritional rehabilitation. The LH and FSH responses to the administration of synthetic gonadotropin-releasing hormone (GnRH) were assessed in 14 patients with anorexia nervosa before, during and following weight gain. In 8 patients studied prior to therapy when they were at 53-64% of idal body weight, the LH response to GnRH was strikingly impaired while the FSH response was equal to that observed in normally menstruating women. After return to 79-88% of ideal body weight the LH response was improved but remained less than in the normal women. In a second group of 6 patients who were studied 4-11 months after hospital discharge when they were 90-94% of ideal body weight, the LH response was always equal to or greater than that found in the normal women. The serum estradiol concentration was low in all of the patients and remained less than 33 pg/ml even in those who returned to more than 90% of ideal body weight. Treatment with 25 mug of ethinyl estradiol for 3 days prior to repeat administration of GnRH did not result in a statistically significant increase of the LH or FSH responses above those before treatment. These data suggest changes occur in both hypothalamic and pituitary regulation of gonadotropin secretion in the course of nutritional rehabilitation of patients with anorexia nervosa and that synthesis and secretion of LH and FSH are modulated independently at the level of the pituitary gland."}
{"id": "PMID:1097462", "title": "Effect of synthetic gonadotropin-releasing hormone (GnRH) in a patient with the \"fertile eunuch\" syndrome.", "content": "A patient with isolated LH deficiency (\"fertile eunuch\" syndrome) was given synthetic gonadotropin releasing hormone (GnRH) by a single intravenous injection and serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone and dihydrotestosterone were measured. A significant rise in both gonadotropins was found after GnRH and the increase was similar to that reported for normal men. No significant change was noted in the serum androgen levels. The patient was re-tested with another single intravenous injection after a 6-h infusion of GnRH two days later. The rise in serum Fsh and LH levels during the infusion was greater than with the initial bolus. A further increase in LH but not FSH concentration was noted when a 150 mug bolus was given at the end of the infusion. Acute (4 day) treatment with human chorionic gonadotropin (hCG) resulted in an increase in the serum testosterone and dihydrotestosterone levels to those observed in normal adult males. The \"fertile eunuch\" syndrome thus appears to be a hypothalamic disorder. It is consistent with the concept of more than one hypothalamic factor controlling gonadotropin secretion. Other possibilities would be deficient or defective production of a releasing factor affecting one or both gonadotropins. The lack of a definite response of serum androgens to GnRH would appear to be secondary to a decreased number of differentiated Leyding cells.", "contents": "Effect of synthetic gonadotropin-releasing hormone (GnRH) in a patient with the \"fertile eunuch\" syndrome. A patient with isolated LH deficiency (\"fertile eunuch\" syndrome) was given synthetic gonadotropin releasing hormone (GnRH) by a single intravenous injection and serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone and dihydrotestosterone were measured. A significant rise in both gonadotropins was found after GnRH and the increase was similar to that reported for normal men. No significant change was noted in the serum androgen levels. The patient was re-tested with another single intravenous injection after a 6-h infusion of GnRH two days later. The rise in serum Fsh and LH levels during the infusion was greater than with the initial bolus. A further increase in LH but not FSH concentration was noted when a 150 mug bolus was given at the end of the infusion. Acute (4 day) treatment with human chorionic gonadotropin (hCG) resulted in an increase in the serum testosterone and dihydrotestosterone levels to those observed in normal adult males. The \"fertile eunuch\" syndrome thus appears to be a hypothalamic disorder. It is consistent with the concept of more than one hypothalamic factor controlling gonadotropin secretion. Other possibilities would be deficient or defective production of a releasing factor affecting one or both gonadotropins. The lack of a definite response of serum androgens to GnRH would appear to be secondary to a decreased number of differentiated Leyding cells."}
{"id": "PMID:1097463", "title": "The role of estrogen in the modulation of pituitary sensitivity of LRF (luteinizing hormone-releasing factor) in men.", "content": "The role of estradiol in modulating pituitary gonadotropin release in the human male was studied by evaluating the effects of clomiphene on the pituitary gonadotropin response to synthetic LRF (150 mug) in 6 eugonadal men. Administration of clomiphene (100 mg single daily dose time 5 days) induced a significant elevation of the basal levels of LH, FSH, estradiol and testosterone. However, the pituitary release of LH and FSH in response to LRF was markedly diminished by the clomiphene treatment. This finding suggests that in men, as in women, endogenous estradiol provides feedback regulation of gonadotropin output by the pituitary.", "contents": "The role of estrogen in the modulation of pituitary sensitivity of LRF (luteinizing hormone-releasing factor) in men. The role of estradiol in modulating pituitary gonadotropin release in the human male was studied by evaluating the effects of clomiphene on the pituitary gonadotropin response to synthetic LRF (150 mug) in 6 eugonadal men. Administration of clomiphene (100 mg single daily dose time 5 days) induced a significant elevation of the basal levels of LH, FSH, estradiol and testosterone. However, the pituitary release of LH and FSH in response to LRF was markedly diminished by the clomiphene treatment. This finding suggests that in men, as in women, endogenous estradiol provides feedback regulation of gonadotropin output by the pituitary."}
{"id": "PMID:1097467", "title": "Pressure tension, and force of closure of the human lower esophageal sphincter and esophagus.", "content": "The mechanical characteristics of the circular muscle of the human lower esophageal sphincter and esophagus were studied in subjects with competent and incompetent sphincters. Pressure-diameter curves were constructed by producing various degrees of circumferential stretch with pressure-measuring probes of increasing diameter. The circumferential membrane tension (force of closure) and the circumferential stress (muscle tension) of the circular muscle layer were also calculated from these data. The pressure-diameter curves of competent and incompetent sphincters were different in magnitude and shape. Incompetent sphincters had lower pressures at all diameters, with pressure gradually increasing with larger probe diameter. In contrast for competent sphincters the pressure was highest near closure, with an initial decline and then an increase in pressure with increasing probe diameter. Both shape and magnitude of pressure-diameter curves of competent and incompetent sphincters were interchangeable when manipulated by pharmacologic agents. Urecholine increased the pressures and changed the incompetent pressure-diameter curve to the levels of the competent sphincter; conversely. Pro-Banthine decreased pressures and changed the shape of the competent pressure-diameter curve to the levels of the incompetent sphincter. Force of closure and circular muscle tension curves of competent and incompetent sphincters were similar in shape but were higher at all diameters for competent sphincters. Force of closure and circular muscle tension increased with larger probe diameter. However, the diameter of optimal tension development was larger than the largest probe used and certainly far from closure. Fundoplication increased the magnitude and changed the shape of the incompetent pressure-diameter curve to one similar to a competent curve. This pressure change was associated with an increase in the force of closure, suggesting that fundopliation modified the length-force of closure characteristics of the incompetent spincter.", "contents": "Pressure tension, and force of closure of the human lower esophageal sphincter and esophagus. The mechanical characteristics of the circular muscle of the human lower esophageal sphincter and esophagus were studied in subjects with competent and incompetent sphincters. Pressure-diameter curves were constructed by producing various degrees of circumferential stretch with pressure-measuring probes of increasing diameter. The circumferential membrane tension (force of closure) and the circumferential stress (muscle tension) of the circular muscle layer were also calculated from these data. The pressure-diameter curves of competent and incompetent sphincters were different in magnitude and shape. Incompetent sphincters had lower pressures at all diameters, with pressure gradually increasing with larger probe diameter. In contrast for competent sphincters the pressure was highest near closure, with an initial decline and then an increase in pressure with increasing probe diameter. Both shape and magnitude of pressure-diameter curves of competent and incompetent sphincters were interchangeable when manipulated by pharmacologic agents. Urecholine increased the pressures and changed the incompetent pressure-diameter curve to the levels of the competent sphincter; conversely. Pro-Banthine decreased pressures and changed the shape of the competent pressure-diameter curve to the levels of the incompetent sphincter. Force of closure and circular muscle tension curves of competent and incompetent sphincters were similar in shape but were higher at all diameters for competent sphincters. Force of closure and circular muscle tension increased with larger probe diameter. However, the diameter of optimal tension development was larger than the largest probe used and certainly far from closure. Fundoplication increased the magnitude and changed the shape of the incompetent pressure-diameter curve to one similar to a competent curve. This pressure change was associated with an increase in the force of closure, suggesting that fundopliation modified the length-force of closure characteristics of the incompetent spincter."}
{"id": "PMID:1097468", "title": "Serological diagnosis of congenital toxoplasmosis.", "content": "A comparison of the indirect haemagglutination test (IHAT) with the dye test (DT) in 23 infants with congenital toxoplasmosis showed that in the early part of the first year of life the IHAT titres were nearly all lower than the DT titres. In six cases the IHAT was negative or the titre was so low that the cases could have been missed in routine screening. Later in the first year the IHAT titres became higher than the DT and remained so. The infants belonging to mothers with a high IHAT and therefore assumed to have become infected early in pregnancy had severe infections which included all the cases of hydrocephalus. Infants of mothers with low IHAT titres relative to the DT and therefore thought to have been infected later in pregnancy had generalized, mild or subclinical infection. With some difficulty a suitable commercial conjugated anti-IgM serum was found that gave positive results in immunofluorescent antibody tests for specific IgM antibody with all the infected infants and their mothers throughout the first year and negative results in uninfected infants and their mothers.", "contents": "Serological diagnosis of congenital toxoplasmosis. A comparison of the indirect haemagglutination test (IHAT) with the dye test (DT) in 23 infants with congenital toxoplasmosis showed that in the early part of the first year of life the IHAT titres were nearly all lower than the DT titres. In six cases the IHAT was negative or the titre was so low that the cases could have been missed in routine screening. Later in the first year the IHAT titres became higher than the DT and remained so. The infants belonging to mothers with a high IHAT and therefore assumed to have become infected early in pregnancy had severe infections which included all the cases of hydrocephalus. Infants of mothers with low IHAT titres relative to the DT and therefore thought to have been infected later in pregnancy had generalized, mild or subclinical infection. With some difficulty a suitable commercial conjugated anti-IgM serum was found that gave positive results in immunofluorescent antibody tests for specific IgM antibody with all the infected infants and their mothers throughout the first year and negative results in uninfected infants and their mothers."}
{"id": "PMID:1097469", "title": "Effects of delayed culture on semiquantitative urinary bacteriology results.", "content": "Urine specimens received in the laboratory were cultured at intervals of two hours on three successive occasions. During the intervening period the specimens were left at room temperature. After 24 hours' incubation counts were done, and it was observed that the significance of bacterial growth was not altered by delay in culture.", "contents": "Effects of delayed culture on semiquantitative urinary bacteriology results. Urine specimens received in the laboratory were cultured at intervals of two hours on three successive occasions. During the intervening period the specimens were left at room temperature. After 24 hours' incubation counts were done, and it was observed that the significance of bacterial growth was not altered by delay in culture."}
{"id": "PMID:1097470", "title": "Effects of minocycline and tetracycline on the vaginal yeast flora.", "content": "Two groups of women with non-specific genital infection were treated for two weeks with tetracycline 250 mg six-hourly and minocycline 100 mg twice daily respectively. Cultures for yeasts were performed before treatment and at the end of the first and second weeks. Before treatment yeasts, mostly Candida albicans, were recovered from 13% of the women. After one and two weeks' treatment yeasts were isolated from 22 and 29% of women treated with tetracycline, and from 19 and 29% of women treated with minocycline. It is concluded that despite inhibition of the growth of yeasts shown by minocycline in vitro, there is no evidence of any significant difference between the actions of tetracycline and minocycline on the vaginal yeast flora.", "contents": "Effects of minocycline and tetracycline on the vaginal yeast flora. Two groups of women with non-specific genital infection were treated for two weeks with tetracycline 250 mg six-hourly and minocycline 100 mg twice daily respectively. Cultures for yeasts were performed before treatment and at the end of the first and second weeks. Before treatment yeasts, mostly Candida albicans, were recovered from 13% of the women. After one and two weeks' treatment yeasts were isolated from 22 and 29% of women treated with tetracycline, and from 19 and 29% of women treated with minocycline. It is concluded that despite inhibition of the growth of yeasts shown by minocycline in vitro, there is no evidence of any significant difference between the actions of tetracycline and minocycline on the vaginal yeast flora."}
{"id": "PMID:1097472", "title": "A study of immunoglobulin M antibody to measles, canine distemper, and rinderpest viruses in sera of patients with subacute sclerosing panencephalitis.", "content": "Seven boys were studied who had the clinical features of subacute sclerosing panencephalitis (SSPE) and whose brain histology was consistent with SSPE. Measles antigen was detected in the seven brains by the direct fluorescent antibody method. Three out of the seven boys had in their sera measles specific immunoglobulin M (IgM) which was detected by the indirect fluorescent antibody method, and the cell receptors for it were acetone stable. A prozone effect was noted in the sera of two patients. The absorption of one patient's serum with Staphylococcus aureus to reduce the titre of immunoglobulin G (IgG) removed the prozone effect. Two of the boys who had high titres of measles specific IgM also had serum IgM which reacted with canine distemper virus antigen but the titres were eightfold lower. None of the boys had detectable rinderpest specific IgM in their sera.", "contents": "A study of immunoglobulin M antibody to measles, canine distemper, and rinderpest viruses in sera of patients with subacute sclerosing panencephalitis. Seven boys were studied who had the clinical features of subacute sclerosing panencephalitis (SSPE) and whose brain histology was consistent with SSPE. Measles antigen was detected in the seven brains by the direct fluorescent antibody method. Three out of the seven boys had in their sera measles specific immunoglobulin M (IgM) which was detected by the indirect fluorescent antibody method, and the cell receptors for it were acetone stable. A prozone effect was noted in the sera of two patients. The absorption of one patient's serum with Staphylococcus aureus to reduce the titre of immunoglobulin G (IgG) removed the prozone effect. Two of the boys who had high titres of measles specific IgM also had serum IgM which reacted with canine distemper virus antigen but the titres were eightfold lower. None of the boys had detectable rinderpest specific IgM in their sera."}
{"id": "PMID:1097473", "title": "Interaction in vitro between synovial cells and autologous lymphocytes and sera from arthritis patients.", "content": "Synovial cells from patients with rheumatoid arthritis (RA) when grown in vitro in media supplemented with 20% fetal calf serum failed to show any difference in growth rate, life span, uptake of tritiated thymidine or cellular and nuclear characteristics when compared with synovial cells from patients with osteoarthritis or other joint diseases grown similarly in 20% serum enriched medium. There was also no evidence that lymphocytes and/or sera from RA patients were more cytotoxic to autologous synovial cells than sera and/or lymphocytes from OA patients. It is unlikely that antisynovial antibodies or lymphocytes from RA patients act as triggers for synovial damage.", "contents": "Interaction in vitro between synovial cells and autologous lymphocytes and sera from arthritis patients. Synovial cells from patients with rheumatoid arthritis (RA) when grown in vitro in media supplemented with 20% fetal calf serum failed to show any difference in growth rate, life span, uptake of tritiated thymidine or cellular and nuclear characteristics when compared with synovial cells from patients with osteoarthritis or other joint diseases grown similarly in 20% serum enriched medium. There was also no evidence that lymphocytes and/or sera from RA patients were more cytotoxic to autologous synovial cells than sera and/or lymphocytes from OA patients. It is unlikely that antisynovial antibodies or lymphocytes from RA patients act as triggers for synovial damage."}
{"id": "PMID:1097474", "title": "Use of a computer for producing microbiological reports and for data storage and processing.", "content": "A simple 'fail-safe' system is described that produces microbiology reports on a computer teleprinter and stores results on the magnetic tapes and discs of a computer for instant retrieval and epidemiological analysis. The system, which has been in operation for over a year, involves the use of a modified conventional NCR request form. The top portion, which is completed manually by the laboratory staff, is coded by writing numbers in hatched boxes. The data thus written on to the bottom portion are transcribed by punch operators on to paper tape and this data input is verified by double punching. The reports are normally produced automatically by the computer terminal telprinter, but in case of mechanical failure the manually completed request forms can be returned to wards and outpatient departments. The system permits a wide choice of options for epidemiological analysis, and six programmes are described, one of which produces a digest of  the overall percentage antibiotic sensitivities of organisms from various sites.", "contents": "Use of a computer for producing microbiological reports and for data storage and processing. A simple 'fail-safe' system is described that produces microbiology reports on a computer teleprinter and stores results on the magnetic tapes and discs of a computer for instant retrieval and epidemiological analysis. The system, which has been in operation for over a year, involves the use of a modified conventional NCR request form. The top portion, which is completed manually by the laboratory staff, is coded by writing numbers in hatched boxes. The data thus written on to the bottom portion are transcribed by punch operators on to paper tape and this data input is verified by double punching. The reports are normally produced automatically by the computer terminal telprinter, but in case of mechanical failure the manually completed request forms can be returned to wards and outpatient departments. The system permits a wide choice of options for epidemiological analysis, and six programmes are described, one of which produces a digest of  the overall percentage antibiotic sensitivities of organisms from various sites."}
{"id": "PMID:1097476", "title": "Study of adjuvant chemotherapy in osteogenic sarcoma.", "content": "Twelve patients with osteosarcoma but without metastases were treated with adjuvant chemotherapy following surgical amputation for the primary lesion. The chemotherapy regimen utilized vincristine, actinomycin D, and cyclophosphamide (in high pulse doses) administered intermittently over a 55-week period. Four of the 12 patients (33%) are surviving with no evidence of disease 54 months or longer from time of surgery. These results suggest that the 3-drug combination used in this study should be considered in the formulation of future multidrug adjuvant chemotherapy programs in the management of patients with osteosarcoma.", "contents": "Study of adjuvant chemotherapy in osteogenic sarcoma. Twelve patients with osteosarcoma but without metastases were treated with adjuvant chemotherapy following surgical amputation for the primary lesion. The chemotherapy regimen utilized vincristine, actinomycin D, and cyclophosphamide (in high pulse doses) administered intermittently over a 55-week period. Four of the 12 patients (33%) are surviving with no evidence of disease 54 months or longer from time of surgery. These results suggest that the 3-drug combination used in this study should be considered in the formulation of future multidrug adjuvant chemotherapy programs in the management of patients with osteosarcoma."}
{"id": "PMID:1097481", "title": "Implications of genetic variation in mouse brain structure for electrode placement by stereotaxic surgery.", "content": "The spatial locations of several forebrain and midbrain fibre tracts have been compared across seven commonly used inbred, hybrid and outbred mouse strains using a series of electrolytic lesions of known positions with respect to a stereotaxic instrument. Highly significant genetic variation was found in the position of lambda, bregma and several major fibre tracts with respect to interaural zero; in the locations of fibre tracts with respect to bregma; and in the location of fibre tracts with respect to each other. It was demonstrated that stereotaxic coordinates of a structure for one strain could not be used for other strains. Errors resulting from improper alignment of the head in the instrument and histological artifact were also determined. These errors showed no significant group differences, but they revealed that within-group variation was substantially affected by such imperfections.", "contents": "Implications of genetic variation in mouse brain structure for electrode placement by stereotaxic surgery. The spatial locations of several forebrain and midbrain fibre tracts have been compared across seven commonly used inbred, hybrid and outbred mouse strains using a series of electrolytic lesions of known positions with respect to a stereotaxic instrument. Highly significant genetic variation was found in the position of lambda, bregma and several major fibre tracts with respect to interaural zero; in the locations of fibre tracts with respect to bregma; and in the location of fibre tracts with respect to each other. It was demonstrated that stereotaxic coordinates of a structure for one strain could not be used for other strains. Errors resulting from improper alignment of the head in the instrument and histological artifact were also determined. These errors showed no significant group differences, but they revealed that within-group variation was substantially affected by such imperfections."}
{"id": "PMID:1097478", "title": "Penfluridol: a long-acting oral neuroleptic. A controlled study.", "content": "The clinical promise of penfluridol as a long-acting oral antipsychotic medication has led to a number of controlled studies designed to verify its usefulness. These studies have been reviewed and compared with a controlled study carried out by the authors. The data obtained from this study have tended to confirm the impression of previous investigators that penfluridol, administered in a dosage of 40 to 80 mg on a once-a-week basis in a single dose, compares favorably with the antipsychotic activity of those neuroleptics requiring administration on a daily basis.", "contents": "Penfluridol: a long-acting oral neuroleptic. A controlled study. The clinical promise of penfluridol as a long-acting oral antipsychotic medication has led to a number of controlled studies designed to verify its usefulness. These studies have been reviewed and compared with a controlled study carried out by the authors. The data obtained from this study have tended to confirm the impression of previous investigators that penfluridol, administered in a dosage of 40 to 80 mg on a once-a-week basis in a single dose, compares favorably with the antipsychotic activity of those neuroleptics requiring administration on a daily basis."}
{"id": "PMID:1097479", "title": "Crossover clinical trial of benapryzine and trihexyphenidyl in Parkinsonian patients.", "content": "A crossover clinical trial of benapryzine and trihexyphenidyl in ten parkinsonian patients during a four-month study is described. The improvement from initial disability ranged from 29.5 to 64.4 per cent for benapryzine and from 27.2 to 64.9 per cent for trihexyphenidyl. The effect of benapryzine and trihexyphenidyl on most parkinsonian symptoms did not differ significantly. Patients taking benapryzine had significantly fewer of the common side effects of trihexyphenidyl but more sialorrhea. It is uncertain whether benapryzine is superior to trihexylphenidyl in the treatment of Parkinson's disease.", "contents": "Crossover clinical trial of benapryzine and trihexyphenidyl in Parkinsonian patients. A crossover clinical trial of benapryzine and trihexyphenidyl in ten parkinsonian patients during a four-month study is described. The improvement from initial disability ranged from 29.5 to 64.4 per cent for benapryzine and from 27.2 to 64.9 per cent for trihexyphenidyl. The effect of benapryzine and trihexyphenidyl on most parkinsonian symptoms did not differ significantly. Patients taking benapryzine had significantly fewer of the common side effects of trihexyphenidyl but more sialorrhea. It is uncertain whether benapryzine is superior to trihexylphenidyl in the treatment of Parkinson's disease."}
{"id": "PMID:1097487", "title": "Guidelines for diet counseling.", "content": "Members of the American Dietetic Association have shown an interest in having some procedural guidance in the process of dietary counseling. Such guidelines, prepared by a committee of the Diet Therapy Section, are presented. They include: a clarification of terminology; requirements for counseling in terms of knowledge, skills, and equipment; recommended procedural steps in counseling; and a supportive bibliography. While these guidelines are intended chiefly for dietitians in clinic and/or ambulatory care situations, the principles, philosophy, and general methodology should be basic to any educational situation, recognizing that circumstances can well present varying limitations.", "contents": "Guidelines for diet counseling. Members of the American Dietetic Association have shown an interest in having some procedural guidance in the process of dietary counseling. Such guidelines, prepared by a committee of the Diet Therapy Section, are presented. They include: a clarification of terminology; requirements for counseling in terms of knowledge, skills, and equipment; recommended procedural steps in counseling; and a supportive bibliography. While these guidelines are intended chiefly for dietitians in clinic and/or ambulatory care situations, the principles, philosophy, and general methodology should be basic to any educational situation, recognizing that circumstances can well present varying limitations."}
{"id": "PMID:1097480", "title": "Effects of protriptyline and perphenazine in neurotic depressed outpatients.", "content": "A four-week comparison of protriptyline 10 mg three times a day and perphenazine 2 mg three times a day, alone and in combination, and a placebo in a group of nonpsychotic depressed outpatients showed no significant differences among any of the treatment groups after both two and four weeks, and a slight trend in favor of the placebo group. A significant positive correlation was observed between changes in hostility and changes in depression, contrary to many commonly held assumptions regarding the relationship between hostility and depression.", "contents": "Effects of protriptyline and perphenazine in neurotic depressed outpatients. A four-week comparison of protriptyline 10 mg three times a day and perphenazine 2 mg three times a day, alone and in combination, and a placebo in a group of nonpsychotic depressed outpatients showed no significant differences among any of the treatment groups after both two and four weeks, and a slight trend in favor of the placebo group. A significant positive correlation was observed between changes in hostility and changes in depression, contrary to many commonly held assumptions regarding the relationship between hostility and depression."}
{"id": "PMID:1097488", "title": "The pregnant adolescent. A review of nutritional research and programs.", "content": "Avialable nutritional research on pregnant adolescents shows that suboptimal food intake is common. Multiple social, psychologic, and economic problems may mitigate against attainment of adequate nutritional status. Use of supplements will raise the level of some nutrients, but in reported studies, their use by girls was erratic, and routine use is not favored by many practitioners. Results of nutritional counseling are mixed. Multidisciplinary programs, usually providing comprehensive prenatal care while enabling girls to continue their education, have been instituted in many areas, reflecting a changed attiude of school officials toward the pregnant adolescent. Most of these programs include the nutrition classes, counseling, or services valuable to mother and child; however, they do not reach all pregnant adolescents and are not a panacea guaranteeing adequate nutrition for them. More research and a national policy to provide optimal nutrition for all pregnant women, including the adolescent, are needed.", "contents": "The pregnant adolescent. A review of nutritional research and programs. Avialable nutritional research on pregnant adolescents shows that suboptimal food intake is common. Multiple social, psychologic, and economic problems may mitigate against attainment of adequate nutritional status. Use of supplements will raise the level of some nutrients, but in reported studies, their use by girls was erratic, and routine use is not favored by many practitioners. Results of nutritional counseling are mixed. Multidisciplinary programs, usually providing comprehensive prenatal care while enabling girls to continue their education, have been instituted in many areas, reflecting a changed attiude of school officials toward the pregnant adolescent. Most of these programs include the nutrition classes, counseling, or services valuable to mother and child; however, they do not reach all pregnant adolescents and are not a panacea guaranteeing adequate nutrition for them. More research and a national policy to provide optimal nutrition for all pregnant women, including the adolescent, are needed."}
{"id": "PMID:1097489", "title": "Ibuprofen versus buffered phenylbutazone in the treatment of osteoarthritis: double-blind trial.", "content": "In a double-blind multiclinic trial, a new nonsteroidal anti-inflammatory agent (ibuprofen) was compared with an established therapeutic agent (phenylbutazone-alka) for the treatment of osteoarthritis. Of the 159 patients from the 17 contributing clinics, 144 completed the four weeks of therapy. More than 60 per cent of them reported improvement in exercise-related pain by week 4, and there was no significant difference between treatment groups. The patients' and the physicians' evaluations of the total state of disease, as well as range-of-motion and functional tests, demonstrated similar degrees of improvement in both treatment groups. The incidence of side effects was within acceptable limits, and the frequency distribution was similar in both groups. Of the 70 reported side effects, 29 were considered by the investigator (blind trial) to be drug-related-11 in association with ibuprofen and 18 with phenylbutazone-alka. Hematologic and blood chemical studies, as well as urine and stool examinations, yielded normal results with the exception of a reduced mean value for serum uric acid and a slightly elevated mean value for SGPT in the phenylbutazone-alka group.", "contents": "Ibuprofen versus buffered phenylbutazone in the treatment of osteoarthritis: double-blind trial. In a double-blind multiclinic trial, a new nonsteroidal anti-inflammatory agent (ibuprofen) was compared with an established therapeutic agent (phenylbutazone-alka) for the treatment of osteoarthritis. Of the 159 patients from the 17 contributing clinics, 144 completed the four weeks of therapy. More than 60 per cent of them reported improvement in exercise-related pain by week 4, and there was no significant difference between treatment groups. The patients' and the physicians' evaluations of the total state of disease, as well as range-of-motion and functional tests, demonstrated similar degrees of improvement in both treatment groups. The incidence of side effects was within acceptable limits, and the frequency distribution was similar in both groups. Of the 70 reported side effects, 29 were considered by the investigator (blind trial) to be drug-related-11 in association with ibuprofen and 18 with phenylbutazone-alka. Hematologic and blood chemical studies, as well as urine and stool examinations, yielded normal results with the exception of a reduced mean value for serum uric acid and a slightly elevated mean value for SGPT in the phenylbutazone-alka group."}
{"id": "PMID:1097490", "title": "Effects of a procaine preparation (Gerovital H3) in hospitalized geriatric patients: a double-blind study.", "content": "The effects of Gerovital H3 (a specially stabilized form of procaine hydrochloride) on geriatric psychiatric patients were assessed in a double-blind study at Bronx State Hospital. The mean age of the subjects was 73 years and the average rating for the severity of organic symptoms was \"moderate.\" During the first six weeks of study, the patients were each given a 5-ml injection of either Gerovital or placebo (saline) intramuscularly three times a week. This dosage was doubled to 10 ml per injection during the second six weeks. Nine Gerovital and 10 control subjects completed the first six weeks; and 6 Gerovital and 7 control subjects completed the entire 12-week study. Objective rating scales were used to evaluate patients on measures of interpersonal functioning, cognitive ability, psychiatric symptoms, and urine and blood chemical findings. All subjects were assessed before treatment and at six weeks and twelve weeks of the study. Side effects were recorded at two-week intervals. On most measures the variability between subjects was quite large, whereas differences between average scores for the two groups usually were small The few significant differences showed no systematic pattern and would be expected to occur by chance alone when so many statistical comparisons are made. The overall results of this double-blind study strongly indicated that, among these hospitalized geriatric patients with organic symptoms, Gerovital H3 had no ameliorative effect on either psychologic or physiologic functioning.", "contents": "Effects of a procaine preparation (Gerovital H3) in hospitalized geriatric patients: a double-blind study. The effects of Gerovital H3 (a specially stabilized form of procaine hydrochloride) on geriatric psychiatric patients were assessed in a double-blind study at Bronx State Hospital. The mean age of the subjects was 73 years and the average rating for the severity of organic symptoms was \"moderate.\" During the first six weeks of study, the patients were each given a 5-ml injection of either Gerovital or placebo (saline) intramuscularly three times a week. This dosage was doubled to 10 ml per injection during the second six weeks. Nine Gerovital and 10 control subjects completed the first six weeks; and 6 Gerovital and 7 control subjects completed the entire 12-week study. Objective rating scales were used to evaluate patients on measures of interpersonal functioning, cognitive ability, psychiatric symptoms, and urine and blood chemical findings. All subjects were assessed before treatment and at six weeks and twelve weeks of the study. Side effects were recorded at two-week intervals. On most measures the variability between subjects was quite large, whereas differences between average scores for the two groups usually were small The few significant differences showed no systematic pattern and would be expected to occur by chance alone when so many statistical comparisons are made. The overall results of this double-blind study strongly indicated that, among these hospitalized geriatric patients with organic symptoms, Gerovital H3 had no ameliorative effect on either psychologic or physiologic functioning."}
{"id": "PMID:1097491", "title": "Treatment of trigeminal neuralgia in the aged by a simplified surgical approach (percutaneous electrocoagulation).", "content": "Trigeminal neuralgia can be excruciatingly painful. For aged patients, among whom trigeminal neuralgia most commonly occurs, medical therapy may often afford relief of pain. However, since this disorder is likely to become worse with increasing age, medical therapy eventually may fail because of increasing drug requirements and intolerable side effects. Major forms of surgical treatment requiring craniotomy may afford longstanding relief but are associated with significant side effects in 3-5 per cent of cases. For these reasons the authors began to test a new surgical approach, i.e., stereotaxic percutaneous electrocoagulation of the trigeminal nerve. More than 300 patients have been treated by this technique in the past five years. Successive radiofrequency lesions allow the production of a graded sensory deficit sufficient to relieve pain while preserving touch and motor function in the face. Unnecessary suffering from the severe pain of trigeminal neuralgia need not occur because of age or a debilitated condition which might ordinarily preclude surgical treatment. More than 100 patients over 65 years of age (4 of them older than 90) have been treated. Of this group, 93 per cent reported good to excellent results. Seven per cent have had some recurrent pain during the follow-up period; however, recoagulation has been required in only 5 per cent of these patients. Recoagulation may be readily performed in any patient who shows evidence of fading of the sensory deficit. The electrocoagulation technique and the results in elderly patients are discussed.", "contents": "Treatment of trigeminal neuralgia in the aged by a simplified surgical approach (percutaneous electrocoagulation). Trigeminal neuralgia can be excruciatingly painful. For aged patients, among whom trigeminal neuralgia most commonly occurs, medical therapy may often afford relief of pain. However, since this disorder is likely to become worse with increasing age, medical therapy eventually may fail because of increasing drug requirements and intolerable side effects. Major forms of surgical treatment requiring craniotomy may afford longstanding relief but are associated with significant side effects in 3-5 per cent of cases. For these reasons the authors began to test a new surgical approach, i.e., stereotaxic percutaneous electrocoagulation of the trigeminal nerve. More than 300 patients have been treated by this technique in the past five years. Successive radiofrequency lesions allow the production of a graded sensory deficit sufficient to relieve pain while preserving touch and motor function in the face. Unnecessary suffering from the severe pain of trigeminal neuralgia need not occur because of age or a debilitated condition which might ordinarily preclude surgical treatment. More than 100 patients over 65 years of age (4 of them older than 90) have been treated. Of this group, 93 per cent reported good to excellent results. Seven per cent have had some recurrent pain during the follow-up period; however, recoagulation has been required in only 5 per cent of these patients. Recoagulation may be readily performed in any patient who shows evidence of fading of the sensory deficit. The electrocoagulation technique and the results in elderly patients are discussed."}
{"id": "PMID:1097493", "title": "The water agar test: a new test to measure the bacteriological quality of cream.", "content": "A new test, the water agar test, is described that gives a qualitative index of the presence of bacteria that indicate contamination of the cream, poor storage conditions or both of these factors. The method is simple and requires little equipment. The bacteria grow in a film of diluted cream adsorbed on the surface of a non-nutrient base. After incubation at 30 +/- 0-5 degrees C. for 18-20 hr., a proteolytic and mucoid colony count is obtained which has the same percentage coefficient of variance as a standard plate count. An examination of the effect of storage at different temperatures on the types of bacteria present in cream showed that of all the tests done initially, only the water agar test could predict subsequent bacterial growth with any consistency. The multiplication of presumptive coliform organisms occurred even at 3-5 degrees C. Irrespective of the colony count, the methylene blue reduction time was not shorter than 7 1/2 hr. unless the bacteria were in the logarithmic phase of growth when sampled. A survey was made of the bacterial flora of 188 retail samples of double cream of 15 different brands. The age of the samples varied from freshly separated cream to cream that had been kept in the shop for a day longer than that recommended for sale. The water agar test was compared with the colony count, the presumptive coliform test, a confirmatory coliform count in violet red-bile agar, a lipolytic colony count, a staphylococcal count and the methylene blue reduction test.", "contents": "The water agar test: a new test to measure the bacteriological quality of cream. A new test, the water agar test, is described that gives a qualitative index of the presence of bacteria that indicate contamination of the cream, poor storage conditions or both of these factors. The method is simple and requires little equipment. The bacteria grow in a film of diluted cream adsorbed on the surface of a non-nutrient base. After incubation at 30 +/- 0-5 degrees C. for 18-20 hr., a proteolytic and mucoid colony count is obtained which has the same percentage coefficient of variance as a standard plate count. An examination of the effect of storage at different temperatures on the types of bacteria present in cream showed that of all the tests done initially, only the water agar test could predict subsequent bacterial growth with any consistency. The multiplication of presumptive coliform organisms occurred even at 3-5 degrees C. Irrespective of the colony count, the methylene blue reduction time was not shorter than 7 1/2 hr. unless the bacteria were in the logarithmic phase of growth when sampled. A survey was made of the bacterial flora of 188 retail samples of double cream of 15 different brands. The age of the samples varied from freshly separated cream to cream that had been kept in the shop for a day longer than that recommended for sale. The water agar test was compared with the colony count, the presumptive coliform test, a confirmatory coliform count in violet red-bile agar, a lipolytic colony count, a staphylococcal count and the methylene blue reduction test."}
{"id": "PMID:1097494", "title": "Quality control tests of two salmonella enrichment media using different inocula.", "content": "Inocula of polluted water naturally infected with salmonellas effectively distinguished six brands of selenite and six brands of tetrathionate enrichment media into satisfactory and unsatisfactory categories. Minimal inocula of pure cultures differentiated the tetrathionates, but not the selenites. Inocula of naturally infected chicken giblets suggested that there was a difference between two comparable brands of tetrathionate, but this was not statistically significant. The difference was, however, clearly demonstrated by minimal inocula of pure cultures. Intensive investigation of two inferior tetrathionates revealed inhomogeneity in the distribution of brilliant green in one bottle of one brand. The importance of the salmonella serotype and even the colonial variant used for the pure culture inoculum was also demonstrated.", "contents": "Quality control tests of two salmonella enrichment media using different inocula. Inocula of polluted water naturally infected with salmonellas effectively distinguished six brands of selenite and six brands of tetrathionate enrichment media into satisfactory and unsatisfactory categories. Minimal inocula of pure cultures differentiated the tetrathionates, but not the selenites. Inocula of naturally infected chicken giblets suggested that there was a difference between two comparable brands of tetrathionate, but this was not statistically significant. The difference was, however, clearly demonstrated by minimal inocula of pure cultures. Intensive investigation of two inferior tetrathionates revealed inhomogeneity in the distribution of brilliant green in one bottle of one brand. The importance of the salmonella serotype and even the colonial variant used for the pure culture inoculum was also demonstrated."}
{"id": "PMID:1097495", "title": "Bacteriological tests as indices for the development of off-flavours in cream.", "content": "Bacteriological tests alone are not capable of predicting the keeping quality of cream, since taints can develop which are due to non-bacterial action. When spoilage is due to bacterial growth, the water agar test was found to be more accurate than other tests that were examined.", "contents": "Bacteriological tests as indices for the development of off-flavours in cream. Bacteriological tests alone are not capable of predicting the keeping quality of cream, since taints can develop which are due to non-bacterial action. When spoilage is due to bacterial growth, the water agar test was found to be more accurate than other tests that were examined."}
{"id": "PMID:1097496", "title": "Citrobacter koseri. I. An extended antigenic scheme for Citrobacter koser (syn. C. diversus, Levinea malonatica).", "content": "The names Citrobacter koseri, Levinea malonatica and Citrobacter diversus are synonymous. As a result of the study of representative strains of these organisms an antigenic scheme comprising seven O antigens has been proposed. The examination of a further 165 strains from clinical sources suggests that an additional seven antigens should be included in the scheme. The serological and biochemical reactions of the test strains for all 14 O antigens are described.", "contents": "Citrobacter koseri. I. An extended antigenic scheme for Citrobacter koser (syn. C. diversus, Levinea malonatica). The names Citrobacter koseri, Levinea malonatica and Citrobacter diversus are synonymous. As a result of the study of representative strains of these organisms an antigenic scheme comprising seven O antigens has been proposed. The examination of a further 165 strains from clinical sources suggests that an additional seven antigens should be included in the scheme. The serological and biochemical reactions of the test strains for all 14 O antigens are described."}
{"id": "PMID:1097497", "title": "The effect of thermal pollution on the distribution of Naegleria fowleri.", "content": "The distribution in the environment of Naegleria fowleri, the causal agent of primary amoebic meningoencephalitis has been investigated in this study. N. fowleri was isolated only from a thermally polluted canal. These amoebaflagellates were not isolated from another thermally polluted canal in the neighbourhood indicating that, apart from high temperature, other factors are involved in the selective proliferation of N. fowleri. This species was absent in all other samples originating from two canals, a stream, two lakes, several reservoirs and slow sandfilters of a water supply service and also a water distribution network. Many other amoebae able to grow at 42 degrees C. were found in different places. Most of the N. fowleri strains isolated were not virulent for mice, although they showed all the characteristics of the pathogenic strains.", "contents": "The effect of thermal pollution on the distribution of Naegleria fowleri. The distribution in the environment of Naegleria fowleri, the causal agent of primary amoebic meningoencephalitis has been investigated in this study. N. fowleri was isolated only from a thermally polluted canal. These amoebaflagellates were not isolated from another thermally polluted canal in the neighbourhood indicating that, apart from high temperature, other factors are involved in the selective proliferation of N. fowleri. This species was absent in all other samples originating from two canals, a stream, two lakes, several reservoirs and slow sandfilters of a water supply service and also a water distribution network. Many other amoebae able to grow at 42 degrees C. were found in different places. Most of the N. fowleri strains isolated were not virulent for mice, although they showed all the characteristics of the pathogenic strains."}
{"id": "PMID:1097498", "title": "Prolongation of allograft survival in mice by administration of anti-Thy 1 serum. I. Mediation by in vivo activation of regulatory T cells.", "content": "A single 0.5-ml dose of anti-Thy 1.2 serum raised in AKR (H-2k, Thy 1.1) mice by immunization with C3H (H-2k, Thy 1.2) thymocytes prolonged mean graft survival (MGS) of C57BL/L (H-2b, Thy 1.2) skin on BALB/c (H-2d, Thy, 1.2) mice from 9.2 to 13.0 days (p less than 0.01). Absorption of anti-Thy 1.2 serum with C3H but not AKR brain removed this activity. MGS was prolonged by anti-Thy 1.2 serum given as long as 2 weeks before grafting. Four separately raised anti-Thy 1.2 preparations prolonged MGS, one to a dilution of 1:128. Anti-Thy 1.1 serum prolonged MGS on AKR mice but not on BALB/c mice. Thymectomy 3 weeks or more before graft placement or treatment of BALB/c mice with anti-lymphocyte serum (ALS) diminished the effectiveness of anti-Thy 1.2 serum. Furthermore, infusion of 25 times 10(6) spleen cells from BALB/c mice given anti-Thy 1.2 serum prolonged MGS on syngeneic recipients to 15.6 days whereas spleen cells from mice given no treatment or ALS had no effect. Thymocytes from anti-Thy 1.2 serum treated donors were ineffective in prolonging MGS on transfer. Transfer or serum from anti-Thy 1.2 serum-treated mice also had no effect. The transfer of graft prolongation with lymphoid cells from anti-Thy 1 serum treated mice suggests anti-Thy 1 serum may prolong MGS by activating suppressor T cells.", "contents": "Prolongation of allograft survival in mice by administration of anti-Thy 1 serum. I. Mediation by in vivo activation of regulatory T cells. A single 0.5-ml dose of anti-Thy 1.2 serum raised in AKR (H-2k, Thy 1.1) mice by immunization with C3H (H-2k, Thy 1.2) thymocytes prolonged mean graft survival (MGS) of C57BL/L (H-2b, Thy 1.2) skin on BALB/c (H-2d, Thy, 1.2) mice from 9.2 to 13.0 days (p less than 0.01). Absorption of anti-Thy 1.2 serum with C3H but not AKR brain removed this activity. MGS was prolonged by anti-Thy 1.2 serum given as long as 2 weeks before grafting. Four separately raised anti-Thy 1.2 preparations prolonged MGS, one to a dilution of 1:128. Anti-Thy 1.1 serum prolonged MGS on AKR mice but not on BALB/c mice. Thymectomy 3 weeks or more before graft placement or treatment of BALB/c mice with anti-lymphocyte serum (ALS) diminished the effectiveness of anti-Thy 1.2 serum. Furthermore, infusion of 25 times 10(6) spleen cells from BALB/c mice given anti-Thy 1.2 serum prolonged MGS on syngeneic recipients to 15.6 days whereas spleen cells from mice given no treatment or ALS had no effect. Thymocytes from anti-Thy 1.2 serum treated donors were ineffective in prolonging MGS on transfer. Transfer or serum from anti-Thy 1.2 serum-treated mice also had no effect. The transfer of graft prolongation with lymphoid cells from anti-Thy 1 serum treated mice suggests anti-Thy 1 serum may prolong MGS by activating suppressor T cells."}
{"id": "PMID:1097499", "title": "Endotoxin-stimulated spleen cells: characterization of the responding cells.", "content": "The percentage of endotoxin-stimulated lymphoblasts possessing C3 receptors (CR) decreases during a 72-hr period of in vitro cultivation. In studies reported here we attempted to determine whether the drop in the percentage of CR+ blast cells resulted from receptor loss by transformed cells or whether it resulted from the proliferation of additional blast cell types which lacked the CR. Using methods which permitted simultaneous detection of cytoplasmic IgM, the presence of the CR, and 3H-thymidine uptake we found that: a) LPS stimulation resulted in a progressive increase in the number of IgM+ blasts, b) these constituted approximately 30% of the total cell population at 72 hr after LPS stimulation, c) of the IgM+ blasts 72 to 84% were CR+ and 16 to 28% were CR- at 72 hr, d) from 48 to 72 hr there was no decrease in the percentage of IgM+ blasts possessing the CR, and e) an additional blast cell type was observed which incorporated 3H-thymidine but which was IgM- and lacked the CR. These cells constituted from 11 to 27% of total 3H-thymidine+ cells at 72 hr after LPS stimulation. We conclude that the decrease in the percentage of CR+ blasts in LPS-stimulated spleen cell cultures is not due to receptor loss from IgM+ blasts cells but apparently is partly due to the stimulation and proliferation of other cell type(s) which lack(s) the CR. This cell(s), although its functional identity is unknown, does not appear to be of thymic origin. The heterogeneity of the markers on LPS-stimulated blast cells suggests that a heterogeneous population of cells responds to LPS.", "contents": "Endotoxin-stimulated spleen cells: characterization of the responding cells. The percentage of endotoxin-stimulated lymphoblasts possessing C3 receptors (CR) decreases during a 72-hr period of in vitro cultivation. In studies reported here we attempted to determine whether the drop in the percentage of CR+ blast cells resulted from receptor loss by transformed cells or whether it resulted from the proliferation of additional blast cell types which lacked the CR. Using methods which permitted simultaneous detection of cytoplasmic IgM, the presence of the CR, and 3H-thymidine uptake we found that: a) LPS stimulation resulted in a progressive increase in the number of IgM+ blasts, b) these constituted approximately 30% of the total cell population at 72 hr after LPS stimulation, c) of the IgM+ blasts 72 to 84% were CR+ and 16 to 28% were CR- at 72 hr, d) from 48 to 72 hr there was no decrease in the percentage of IgM+ blasts possessing the CR, and e) an additional blast cell type was observed which incorporated 3H-thymidine but which was IgM- and lacked the CR. These cells constituted from 11 to 27% of total 3H-thymidine+ cells at 72 hr after LPS stimulation. We conclude that the decrease in the percentage of CR+ blasts in LPS-stimulated spleen cell cultures is not due to receptor loss from IgM+ blasts cells but apparently is partly due to the stimulation and proliferation of other cell type(s) which lack(s) the CR. This cell(s), although its functional identity is unknown, does not appear to be of thymic origin. The heterogeneity of the markers on LPS-stimulated blast cells suggests that a heterogeneous population of cells responds to LPS."}
{"id": "PMID:1097500", "title": "Studies on leukocyte migration inhibiton: the role of E rosette-forming cells in specific antigen-induced inhibition of migration.", "content": "The role of E rosette-forming cells in leukocyte migration inhibition was investigated with human buffy coat cells fractionated by combined E rosette formation and Ficoll-Hypaque (FH) density gradient centrifugation. The migration of unfractionated dextran-sedimented leukocytes and FH-separated mononuclear cells (MN) were equally inhibited in the presence of Escherichia coli somatic antigen (E. coli), purified protein derivative of tuberculin (PPD) and influenza virus antigen (Flu). Polymorphonuclear leukocytes (PMN) separated from whole leukocytes by one FH density gradient fractionation contained greater than 1.0% E rosette-forming cells and were inhibited by E. coli and PPD. PMN submitted to three transits of FH (DF III) contained less than or equal to 0.02% E rosette forming cells and were unaffected by each of the three antigens. The migration of mixtures of 10% MN and 90DF III cells were inhibited in the presence of specific antigen, while MN depleted of E rosette-forming cells (less than or equal to 0.5%) pooled with equal numbers of DF III cells did not respond to any antigen by migration inhibition. These results show that PMN purified by three transits of FH are unable to respond to specific antigen by inhibition of migration from microcapillary tubes, and specific antigen-induced inhibition of migration of peripheral blood leukocytes does not occur in the absence of D rosette-forming cells in normal subjects.", "contents": "Studies on leukocyte migration inhibiton: the role of E rosette-forming cells in specific antigen-induced inhibition of migration. The role of E rosette-forming cells in leukocyte migration inhibition was investigated with human buffy coat cells fractionated by combined E rosette formation and Ficoll-Hypaque (FH) density gradient centrifugation. The migration of unfractionated dextran-sedimented leukocytes and FH-separated mononuclear cells (MN) were equally inhibited in the presence of Escherichia coli somatic antigen (E. coli), purified protein derivative of tuberculin (PPD) and influenza virus antigen (Flu). Polymorphonuclear leukocytes (PMN) separated from whole leukocytes by one FH density gradient fractionation contained greater than 1.0% E rosette-forming cells and were inhibited by E. coli and PPD. PMN submitted to three transits of FH (DF III) contained less than or equal to 0.02% E rosette forming cells and were unaffected by each of the three antigens. The migration of mixtures of 10% MN and 90DF III cells were inhibited in the presence of specific antigen, while MN depleted of E rosette-forming cells (less than or equal to 0.5%) pooled with equal numbers of DF III cells did not respond to any antigen by migration inhibition. These results show that PMN purified by three transits of FH are unable to respond to specific antigen by inhibition of migration from microcapillary tubes, and specific antigen-induced inhibition of migration of peripheral blood leukocytes does not occur in the absence of D rosette-forming cells in normal subjects."}
{"id": "PMID:1097501", "title": "Kinetic study of T lymphocytes after sensitization against soluble antigen. I. Separation on density gradients.", "content": "Sensitized T cells in inguinal lymph nodes (LN) of rats immunized with ovalbumin and adjuvant can be separated on BSA density gradients from T cells responding to Con A and PHA. Further, these OA-sensitized T cells are separable from B memory cells responsive to OA. On days 9 and 17 after sensitization, OA-reactive cells peak and are found in enriched numbers in the lighter bands A + B (B-band), and C, respectively whereas Con A and PHA reactive cells, on both days, are concentrated in bands C and D, respectively. On day 9 at least 20 to 30% of B-band cells can reinitiate DNA synthesis and produce high concentrations of lymphotoxin in response to OA in culture, but show only a marginal blastogenic response to Con A or PHA and cannot produce lymphotoxin in response to PHA. These cells are defined as OA-sensitized T cells because a) treatment with rat isoantiserum specific for T lymphocytes plus complement abolished their ability to incorporate 3H-thymidine in response to OA and Con A or PHA; b) membrane-bound immunoglobulin could not detected with indirect immunofluorescence staining; c) histologic studies demonstrate massive hypertrophy of thymus-dependent paracortical areas and absence of germinal centers in inguinal LN at this time, and d) they produce lymphotoxin after challenge with OA, but not with PHA, whereas cells of the other bands produce lymphotoxin in response to PHA, but not to OA. Addition of purified macrophages to the various cultures did not affect the levels of response observed. Day 17 C-band cells are regarded as B-memory cells because treatment with T-specific antiserum plus complement enhanced their response to OA, many germinal centers now are present in inguinal LN, and a significant proportion of cells of bands B and C, negative on day 9, show positive immunofluorescence staining at this time.", "contents": "Kinetic study of T lymphocytes after sensitization against soluble antigen. I. Separation on density gradients. Sensitized T cells in inguinal lymph nodes (LN) of rats immunized with ovalbumin and adjuvant can be separated on BSA density gradients from T cells responding to Con A and PHA. Further, these OA-sensitized T cells are separable from B memory cells responsive to OA. On days 9 and 17 after sensitization, OA-reactive cells peak and are found in enriched numbers in the lighter bands A + B (B-band), and C, respectively whereas Con A and PHA reactive cells, on both days, are concentrated in bands C and D, respectively. On day 9 at least 20 to 30% of B-band cells can reinitiate DNA synthesis and produce high concentrations of lymphotoxin in response to OA in culture, but show only a marginal blastogenic response to Con A or PHA and cannot produce lymphotoxin in response to PHA. These cells are defined as OA-sensitized T cells because a) treatment with rat isoantiserum specific for T lymphocytes plus complement abolished their ability to incorporate 3H-thymidine in response to OA and Con A or PHA; b) membrane-bound immunoglobulin could not detected with indirect immunofluorescence staining; c) histologic studies demonstrate massive hypertrophy of thymus-dependent paracortical areas and absence of germinal centers in inguinal LN at this time, and d) they produce lymphotoxin after challenge with OA, but not with PHA, whereas cells of the other bands produce lymphotoxin in response to PHA, but not to OA. Addition of purified macrophages to the various cultures did not affect the levels of response observed. Day 17 C-band cells are regarded as B-memory cells because treatment with T-specific antiserum plus complement enhanced their response to OA, many germinal centers now are present in inguinal LN, and a significant proportion of cells of bands B and C, negative on day 9, show positive immunofluorescence staining at this time."}
{"id": "PMID:1097502", "title": "Physicochemical characterization of chemotatic lymphokines produced by human T and B lymphocytes.", "content": "Ostensibly pure populations of T and B lymphocytes were separated from peripheral human blood by a sequential rosetting method. These cells, and mononuclear leukocyte (MNL) cell preparations (60 to 85% T cells), were cultured in vitro and the supernatants of these cultures were assayed for chemotactic lymphokine (CTX) activity. B lymphocytes were activated to produce CTX by binding erythrocytes sensitized with antibody and complement (EAC) to their C3 receptors and phytohemagglutinin (PHA) stimulation of isolated T cells was used to produce T cell-derived CTX. Sephadex G-100 gel filtration chromatography of supernatants obtained from T and B cell cultures showed that both cell types produce a CTX of approximate molecular weight 12,500 daltons. This result is identical to that previously obtained in characterizing the CTX produced by buffy coat leukocytes. Isoelectric focusing of lymphocyte supernatants and chemotactically active Sephadex G-100 fractions showed that the CTX produced by B cells and PHA-stimulated MNL contained two peaks of lymphokine activity, a major peak at pH 10.1 and a minor peak at pH 5.6. Additionally, these studies show that the chemotactic complement peptide C5a has an isoelectric point of pH 8.7 and is antigenically distinct from T and B cell-derived CTX, although relatively homogenous by gel filtration, contain two isoelectrically distinct species. In addition, these findings suggest that T and B cells although possessing different membrane receptors and requiring different mechanisms of activation to initiate lymphokine synthesis, produce lymphokines with identical physiocochemical properties.", "contents": "Physicochemical characterization of chemotatic lymphokines produced by human T and B lymphocytes. Ostensibly pure populations of T and B lymphocytes were separated from peripheral human blood by a sequential rosetting method. These cells, and mononuclear leukocyte (MNL) cell preparations (60 to 85% T cells), were cultured in vitro and the supernatants of these cultures were assayed for chemotactic lymphokine (CTX) activity. B lymphocytes were activated to produce CTX by binding erythrocytes sensitized with antibody and complement (EAC) to their C3 receptors and phytohemagglutinin (PHA) stimulation of isolated T cells was used to produce T cell-derived CTX. Sephadex G-100 gel filtration chromatography of supernatants obtained from T and B cell cultures showed that both cell types produce a CTX of approximate molecular weight 12,500 daltons. This result is identical to that previously obtained in characterizing the CTX produced by buffy coat leukocytes. Isoelectric focusing of lymphocyte supernatants and chemotactically active Sephadex G-100 fractions showed that the CTX produced by B cells and PHA-stimulated MNL contained two peaks of lymphokine activity, a major peak at pH 10.1 and a minor peak at pH 5.6. Additionally, these studies show that the chemotactic complement peptide C5a has an isoelectric point of pH 8.7 and is antigenically distinct from T and B cell-derived CTX, although relatively homogenous by gel filtration, contain two isoelectrically distinct species. In addition, these findings suggest that T and B cells although possessing different membrane receptors and requiring different mechanisms of activation to initiate lymphokine synthesis, produce lymphokines with identical physiocochemical properties."}
{"id": "PMID:1097503", "title": "Isolation and characterization of the sheep erythrocyte receptor in man.", "content": "Properties of the sheep erythrocyte receptor on human lymphocytes were examined from two different aspects: inhibition of E-rosette formation by antisera, and extraction of T and non-T cells with 3 M KCl. Antisera to several T cell populations produced significant inhibition of E-rosette formation by human peripheral blood lymphocytes, whereas anti-B cell, anti-beta2-microglobulin, anti-I, and anit-i were ineffective. Sera with activity to HL-A subgroups on the HPBL tested produced complete rosette inhibition, although nonspecific anti-HL-A produced no inhibition. T cell preparations extracted by a 3M KCl technique contained activity that could be bound to SRBC and produce inhibition of rosette formation by HPBL. This inhibitory component could be quickly eluted from the SRBC and retain its inhibitory activity. No inhibitory activity could be demonstrated in extracts of B cells and non-lymphoid cells. These results suggest that 1) T cell-specific and HL-A specific antisera inhibit E-rosette formation; 2) beta2-M is not involved in the SRBC receptor as an active component; and 3) the receptor may be isolated by 3 M KCl extraction, and partially purified by the use of SRBC as a specific immunoadsorbent, without loss of biologic activity.", "contents": "Isolation and characterization of the sheep erythrocyte receptor in man. Properties of the sheep erythrocyte receptor on human lymphocytes were examined from two different aspects: inhibition of E-rosette formation by antisera, and extraction of T and non-T cells with 3 M KCl. Antisera to several T cell populations produced significant inhibition of E-rosette formation by human peripheral blood lymphocytes, whereas anti-B cell, anti-beta2-microglobulin, anti-I, and anit-i were ineffective. Sera with activity to HL-A subgroups on the HPBL tested produced complete rosette inhibition, although nonspecific anti-HL-A produced no inhibition. T cell preparations extracted by a 3M KCl technique contained activity that could be bound to SRBC and produce inhibition of rosette formation by HPBL. This inhibitory component could be quickly eluted from the SRBC and retain its inhibitory activity. No inhibitory activity could be demonstrated in extracts of B cells and non-lymphoid cells. These results suggest that 1) T cell-specific and HL-A specific antisera inhibit E-rosette formation; 2) beta2-M is not involved in the SRBC receptor as an active component; and 3) the receptor may be isolated by 3 M KCl extraction, and partially purified by the use of SRBC as a specific immunoadsorbent, without loss of biologic activity."}
{"id": "PMID:1097504", "title": "Lymphocyte abnormalities in Aleutian disease virus infection of mink: decreased T lymphocyte responses and increased B lymphocyte levels in persistent viral infection.", "content": "Persistence of virus and a marked hypergammaglobulinemia are consistent features observed in mink with Aleutian disease virus infection. These events may result from altered lymphocyte function in infected mink. To test this possibility we determined the ability of lymphocytes from infected mink to respond to phytolectins (PHA and Con A), and a specific antigen (KLH) in a lymphocyte transformation test and quantitated the number of lymphocytes bearing surface immunoglobulin. Infected mink had a significantly lower response than normal mink to PHA and Con A (P less than 0.025), and the difference in the responses increased progressively with time post-infection. Infected mink immunized with KLH also had a decreased ability to respond to this antigen when compared to normal immunized mink (P less than 0.001). The number of lymphocytes bearing surface immunoglobulin was significantly elevated in three of 10 mink infected for 10 weeks, and in all of seven mink infected 32 weeks (P less than 0.001).", "contents": "Lymphocyte abnormalities in Aleutian disease virus infection of mink: decreased T lymphocyte responses and increased B lymphocyte levels in persistent viral infection. Persistence of virus and a marked hypergammaglobulinemia are consistent features observed in mink with Aleutian disease virus infection. These events may result from altered lymphocyte function in infected mink. To test this possibility we determined the ability of lymphocytes from infected mink to respond to phytolectins (PHA and Con A), and a specific antigen (KLH) in a lymphocyte transformation test and quantitated the number of lymphocytes bearing surface immunoglobulin. Infected mink had a significantly lower response than normal mink to PHA and Con A (P less than 0.025), and the difference in the responses increased progressively with time post-infection. Infected mink immunized with KLH also had a decreased ability to respond to this antigen when compared to normal immunized mink (P less than 0.001). The number of lymphocytes bearing surface immunoglobulin was significantly elevated in three of 10 mink infected for 10 weeks, and in all of seven mink infected 32 weeks (P less than 0.001)."}
{"id": "PMID:1097506", "title": "The expression on mouse lymphoid cells of Th-B, an antigen common to mouse B cells and thymus cells.", "content": "A new cell-surface antigen of mouse lymphocytes, which was found earlier on normal and malignant plasma cells and about half the normal thymus cell population, has been shown to be on mature B cells and on the least immunocompetent subpopulation of cells of the thymus. The antigen was first detected by the cytotoxic effect on relevant cells of an in vivo purified rabbit antiserum raised against cells of the mouse IgM-producing plasma cell tumor MOPC-104E. We have now used a cell-transfer method to examine the effect of this antiserum (RantiM104E) on immunocompetent lymphocytes of spleen, including separated splenic B and T cells and thymus and bone marrow cells. We have found the antigen on the immunocompetent B lymphocytes of the spleen, but not on any other of the immunocompetent cells examined. The method involves determining the effect of the antiserum on the development of plaque-forming cells in the spleens of irradiated mice that have received lymphocytes from immune or non-immune donors by treating the lymphocyyes  with RantiM104E antiserum before transfer. Plaque-forming cells are suppressed when antiserum-treated spleen cells are transferred to the irradiated mice.This suppression was found to be due to action on only the splenic B cells. The helper activity of splenic T cells was not reduced significantly by antiserum treatment. Neither was the helper activity of thymus cells similarly treated reduced by the antiserum. We found, in fact, that the subpopulation of the thymus cells which is resistant to the antiserum was more immunocompetent than the total thymus cell population. In addition, the hydrocortisone-resistant thymus cells were also found to be resistant to the cytotoxic action of the antiserum. The antiserum had no effect on bone marrow cells in the cell-transfer procedure. The antigen involved, which we are designating \"Th-B\" appears to be a B cell line marker which appears relatively early during the differentiation of mouse B cells from precursor cells and is lost during maturation of T cells.", "contents": "The expression on mouse lymphoid cells of Th-B, an antigen common to mouse B cells and thymus cells. A new cell-surface antigen of mouse lymphocytes, which was found earlier on normal and malignant plasma cells and about half the normal thymus cell population, has been shown to be on mature B cells and on the least immunocompetent subpopulation of cells of the thymus. The antigen was first detected by the cytotoxic effect on relevant cells of an in vivo purified rabbit antiserum raised against cells of the mouse IgM-producing plasma cell tumor MOPC-104E. We have now used a cell-transfer method to examine the effect of this antiserum (RantiM104E) on immunocompetent lymphocytes of spleen, including separated splenic B and T cells and thymus and bone marrow cells. We have found the antigen on the immunocompetent B lymphocytes of the spleen, but not on any other of the immunocompetent cells examined. The method involves determining the effect of the antiserum on the development of plaque-forming cells in the spleens of irradiated mice that have received lymphocytes from immune or non-immune donors by treating the lymphocyyes  with RantiM104E antiserum before transfer. Plaque-forming cells are suppressed when antiserum-treated spleen cells are transferred to the irradiated mice.This suppression was found to be due to action on only the splenic B cells. The helper activity of splenic T cells was not reduced significantly by antiserum treatment. Neither was the helper activity of thymus cells similarly treated reduced by the antiserum. We found, in fact, that the subpopulation of the thymus cells which is resistant to the antiserum was more immunocompetent than the total thymus cell population. In addition, the hydrocortisone-resistant thymus cells were also found to be resistant to the cytotoxic action of the antiserum. The antiserum had no effect on bone marrow cells in the cell-transfer procedure. The antigen involved, which we are designating \"Th-B\" appears to be a B cell line marker which appears relatively early during the differentiation of mouse B cells from precursor cells and is lost during maturation of T cells."}
{"id": "PMID:1097507", "title": "Antigenic competition between heterologous erythrocytes in mice: interference with competition-inducing effect of sheep erythrocytes by inclusion of non-competing antigen.", "content": "Antigenic competition was studied on reciprocal combinations of non-cross-reacting erythrocyte (RBC) antigens, i.e., sheep erythrocytes (SRBC), horse erythrocytes (HRBC), and chicken erythrocytes (CRBC), as competing antigen or as test antigen. Unlike SRBC and HRBC, CRBC did not induce antigenic competition, and mice previously injected with CRBC showed normal direct and indirect hemolysin plaque-forming cell (PFC) responses to the other RBC subsequently injected. Formaldehyde-treated (F) SRBC that retained the capacity to prime mouse for secondary anti-SRBC antibody response but lost the capacity to elicit primary antibody response also could not induce antigenic competition. CRBC or F-SRBC, i.e., non-competing antigen, included with SRBC interfered with the competition-inducing effect of the latter RBC. When mice that had been injected with SRBC together with either one of the non-competing antigens were subsequently immunized with HRBC their anti-HRBC PFC responses were significantly restored, whereas mice pre-injected with SRBC alone showed markedly suppressed PFC responses to HRBC. Although the non-competing antigen was found not to affect both the direct and indirect PFC responses to the simultaneously injected SRBC, the response of the immune system to the non-competing antigen should alter the concurrent response to SRBC, and probably should prevent the generation of the suppressor thymus-derived (T) lymphocytes, allowing the generation of the helper T lymphocytes for IgM and IgG anti-SRBC antibody formation.", "contents": "Antigenic competition between heterologous erythrocytes in mice: interference with competition-inducing effect of sheep erythrocytes by inclusion of non-competing antigen. Antigenic competition was studied on reciprocal combinations of non-cross-reacting erythrocyte (RBC) antigens, i.e., sheep erythrocytes (SRBC), horse erythrocytes (HRBC), and chicken erythrocytes (CRBC), as competing antigen or as test antigen. Unlike SRBC and HRBC, CRBC did not induce antigenic competition, and mice previously injected with CRBC showed normal direct and indirect hemolysin plaque-forming cell (PFC) responses to the other RBC subsequently injected. Formaldehyde-treated (F) SRBC that retained the capacity to prime mouse for secondary anti-SRBC antibody response but lost the capacity to elicit primary antibody response also could not induce antigenic competition. CRBC or F-SRBC, i.e., non-competing antigen, included with SRBC interfered with the competition-inducing effect of the latter RBC. When mice that had been injected with SRBC together with either one of the non-competing antigens were subsequently immunized with HRBC their anti-HRBC PFC responses were significantly restored, whereas mice pre-injected with SRBC alone showed markedly suppressed PFC responses to HRBC. Although the non-competing antigen was found not to affect both the direct and indirect PFC responses to the simultaneously injected SRBC, the response of the immune system to the non-competing antigen should alter the concurrent response to SRBC, and probably should prevent the generation of the suppressor thymus-derived (T) lymphocytes, allowing the generation of the helper T lymphocytes for IgM and IgG anti-SRBC antibody formation."}
{"id": "PMID:1097508", "title": "Antigen-initiated B lymphocyte differentiation. IV. The adherence properties of antibody-forming cell progenitors from primed and unprimed mice.", "content": "Distinct sub-populations of B lymphocytes may be fractionated by passage through glass-bead columns, due to a temperature-independent adherence of certain cells. The relative adherence properties of AFC-progenitors from spleens of primed or unprimed CBA mice were compared with an adoptive immune assay employing as antigen the hapten NIP on the carrier polymerized flagellin, under conditions where T lymphocytes did not limit the response. The AFC-progenitors for an anti-NIP IgM response from unprimed mice, whether conventional, germ-free, or athymic were more adherent than the majority of cells in the spleen. The AFC-progenitors for an IgG response from hapten-primed mice were less adherent that the majority of spleen cells. The results indicate that there are sub-classes of AFC-progenitors, differing in surface properties and probably corresponding to differences between the \"antigen-inexperienced\" and the \"memory\" B cell populations.", "contents": "Antigen-initiated B lymphocyte differentiation. IV. The adherence properties of antibody-forming cell progenitors from primed and unprimed mice. Distinct sub-populations of B lymphocytes may be fractionated by passage through glass-bead columns, due to a temperature-independent adherence of certain cells. The relative adherence properties of AFC-progenitors from spleens of primed or unprimed CBA mice were compared with an adoptive immune assay employing as antigen the hapten NIP on the carrier polymerized flagellin, under conditions where T lymphocytes did not limit the response. The AFC-progenitors for an anti-NIP IgM response from unprimed mice, whether conventional, germ-free, or athymic were more adherent than the majority of cells in the spleen. The AFC-progenitors for an IgG response from hapten-primed mice were less adherent that the majority of spleen cells. The results indicate that there are sub-classes of AFC-progenitors, differing in surface properties and probably corresponding to differences between the \"antigen-inexperienced\" and the \"memory\" B cell populations."}
{"id": "PMID:1097509", "title": "Antagonism of B lymphocyte mitogenesis by anti-immunoglobulin antibody.", "content": "Anti-mouse-immunoglobulin-antibodies (AMIg) were shown to suppress mitogenesis in B lymphocytes that were cultured with lipopolysaccharide, (LPS), or polymerized-flagellin, (POL). Preincubation of spleen cells with AMIg plus LPS has a greater suppressive effect on a subsequent mitogenic response to LPS than did preincubation with AMIg alone. Preincubation with LPS alone had, if anything, a slight enhancing effect. These experiments suggested that AMIg did not suppress mitogenesis by interference with the initial binding of the mitogen, but rather exerted a separated negative effect that occurred subsequent to, or in the process of, B cell-stimulation.", "contents": "Antagonism of B lymphocyte mitogenesis by anti-immunoglobulin antibody. Anti-mouse-immunoglobulin-antibodies (AMIg) were shown to suppress mitogenesis in B lymphocytes that were cultured with lipopolysaccharide, (LPS), or polymerized-flagellin, (POL). Preincubation of spleen cells with AMIg plus LPS has a greater suppressive effect on a subsequent mitogenic response to LPS than did preincubation with AMIg alone. Preincubation with LPS alone had, if anything, a slight enhancing effect. These experiments suggested that AMIg did not suppress mitogenesis by interference with the initial binding of the mitogen, but rather exerted a separated negative effect that occurred subsequent to, or in the process of, B cell-stimulation."}
{"id": "PMID:1097510", "title": "IgG antibody suppression of the IgM antibody response to Toxoplasma gondii in newborn rabbits.", "content": "Studies were performed in an experimental rabbit model in an attempt to explore the possibility that the absence of an IgM antibody response in human infants congenitally infected with Toxoplasma may be caused by a suppressive effect of IgG antibody passively transferred from the mother. The IgM antibody response to Toxoplasma gondii infection in newborn rabbits previously injected with IgG Toxoplasma antibody was significantly suppressed as determined by an immunofluorescent antibody assay (IgM-IFA). In newborn rabbits infected with Toxoplasma, IgM-IFA antibody was first demonstrated 15 days after infection and reached a peak titer of 1:128 in two animals on days 20 and 25. In newborn rabbits injected with IgG Toxoplasma antibody and then infected with Toxoplasma, the Igm-IFA antibody and then infected with Toxoplasma, the Igm-IFA antibody response was considerably lower; the highest titer was 1:16 and occurred 25 and 30 days after infection. The results of these studies lend support to the concept that maternally transmitted IgG may suppress the IgM antibody response in the fetus and newborn infant.", "contents": "IgG antibody suppression of the IgM antibody response to Toxoplasma gondii in newborn rabbits. Studies were performed in an experimental rabbit model in an attempt to explore the possibility that the absence of an IgM antibody response in human infants congenitally infected with Toxoplasma may be caused by a suppressive effect of IgG antibody passively transferred from the mother. The IgM antibody response to Toxoplasma gondii infection in newborn rabbits previously injected with IgG Toxoplasma antibody was significantly suppressed as determined by an immunofluorescent antibody assay (IgM-IFA). In newborn rabbits infected with Toxoplasma, IgM-IFA antibody was first demonstrated 15 days after infection and reached a peak titer of 1:128 in two animals on days 20 and 25. In newborn rabbits injected with IgG Toxoplasma antibody and then infected with Toxoplasma, the Igm-IFA antibody and then infected with Toxoplasma, the Igm-IFA antibody response was considerably lower; the highest titer was 1:16 and occurred 25 and 30 days after infection. The results of these studies lend support to the concept that maternally transmitted IgG may suppress the IgM antibody response in the fetus and newborn infant."}
{"id": "PMID:1097511", "title": "Pretreatment of murine thymocytes by phytohemagglutinin inhibits the binding of H-concanavalin A.", "content": "The binding of H-concanavalin A (Con A) to cortisone-resistant and normal mouse thymocytes, which greatly differ in mitogenic responsiveness, was compared. Almost equal numbers of 3H-Con A molecules (7 x 10(5) molecules per cell) were found to bind to both cell types, and the rates of H-Con A binding were nearly identical. The binding capacity of thymocytes for H-Con A decreased rapidly after removal from the thymus and incubation in protein free synthetic medium. Similarly, mitogenic responsiveness droped rapidly after cells were exposed to this medium. H-Con A molecules bound to cells were dissociated from them after a brief incubation, about 30 percent of radioactivity being lost in 2 hr with increasing radioactivity being found in the supernatant. When cells were pretreated with PHA-P, which is a T cell mitogen, their mitogenic responsiveness to Con A was suppressed along with H-Con A binding to the cells. In contrast, a B cell mitrogen, LPS, that has a synergistic effect on the Con A mitogenic response in thymocytes had no effect on binding of H-Con A to thymocytes.", "contents": "Pretreatment of murine thymocytes by phytohemagglutinin inhibits the binding of H-concanavalin A. The binding of H-concanavalin A (Con A) to cortisone-resistant and normal mouse thymocytes, which greatly differ in mitogenic responsiveness, was compared. Almost equal numbers of 3H-Con A molecules (7 x 10(5) molecules per cell) were found to bind to both cell types, and the rates of H-Con A binding were nearly identical. The binding capacity of thymocytes for H-Con A decreased rapidly after removal from the thymus and incubation in protein free synthetic medium. Similarly, mitogenic responsiveness droped rapidly after cells were exposed to this medium. H-Con A molecules bound to cells were dissociated from them after a brief incubation, about 30 percent of radioactivity being lost in 2 hr with increasing radioactivity being found in the supernatant. When cells were pretreated with PHA-P, which is a T cell mitogen, their mitogenic responsiveness to Con A was suppressed along with H-Con A binding to the cells. In contrast, a B cell mitrogen, LPS, that has a synergistic effect on the Con A mitogenic response in thymocytes had no effect on binding of H-Con A to thymocytes."}
{"id": "PMID:1097512", "title": "Hyperacute rejection and enhancement of mouse skin grafts by antibodies with a distinct specificity.", "content": "Pure 7S IgG2 was prepared from B6AF1 anti-B10.D2 ascites fluid by affinity chromatography. This preparation was extensively absorbed with B10.D2 red blood cells to remove the antibodies directed against the serologically defined antigens of the major histocompatibility complex. After absorption the serum had retained in vitro cytotoxic activity against 45 percent of B10.D2 spleen cells. The absorbed and unabsorbed 7S IgG2 antibodies were tested for their capacity to induce enhancement and, in the presence of rabbit complement, hyperacute rejection of B10.D2 skin grafts in B6AF1 recipient mice. The enhancing capacity of 7S IgG2 was completely unimpaired after absorption. However, the absorbed preparation was unable to induce hyperacute rejection of the grafts even if very high doses were administered. The results show that hyperacute rejection of skin grafts in this model is mediated by antibodies directed against the serologically defined antigens (i.e., H-2K antigens) of the H-2 complex. They further support the hypothesis that immunologic enhancement is mediated by antibodies directed against Ir-region-as-sociated antigens.", "contents": "Hyperacute rejection and enhancement of mouse skin grafts by antibodies with a distinct specificity. Pure 7S IgG2 was prepared from B6AF1 anti-B10.D2 ascites fluid by affinity chromatography. This preparation was extensively absorbed with B10.D2 red blood cells to remove the antibodies directed against the serologically defined antigens of the major histocompatibility complex. After absorption the serum had retained in vitro cytotoxic activity against 45 percent of B10.D2 spleen cells. The absorbed and unabsorbed 7S IgG2 antibodies were tested for their capacity to induce enhancement and, in the presence of rabbit complement, hyperacute rejection of B10.D2 skin grafts in B6AF1 recipient mice. The enhancing capacity of 7S IgG2 was completely unimpaired after absorption. However, the absorbed preparation was unable to induce hyperacute rejection of the grafts even if very high doses were administered. The results show that hyperacute rejection of skin grafts in this model is mediated by antibodies directed against the serologically defined antigens (i.e., H-2K antigens) of the H-2 complex. They further support the hypothesis that immunologic enhancement is mediated by antibodies directed against Ir-region-as-sociated antigens."}
{"id": "PMID:1097513", "title": "Cytotoxic activity of lymphocytes. V. Role of soluble toxin in macrophage-inhibited cultures of tumor cells.", "content": "Macrophage cell-mediated cytolysis (M-CMC) of tumor cells may be measured by culturing target cells on macrophage monolayers and determining target cell survival by either Rb or H-thymidine incorporation. Using these techniques, one can demonstrate cytotoxicity of nonimmune human and rat macrophages to a variety of tumor cell lines. Toxicity becomes maximal 8 to 16 hr after attaching macrophages to plastic surfaces and disappears after 36 to 48 hr. Addition of Escherichia coli endotoxin at 48 hr restores macrophage toxicity. Culture of macrophages on plastic surfaces also results in the release, nonspecifically, of a soluble cytotoxin whose kinetics of release, disappearance, and reappearance after endotoxin all parallel the M-CMC. The amount of toxin released into cultures containing target cells is markedly reduced, suggesting that it is the mediator of the M-CMC and that it is utilized or absorbed during target destruction. Sephadex G-100 chromatography and treatment with heterologous anti-lymphotoxin can distinguish this toxin from lymphotoxin.", "contents": "Cytotoxic activity of lymphocytes. V. Role of soluble toxin in macrophage-inhibited cultures of tumor cells. Macrophage cell-mediated cytolysis (M-CMC) of tumor cells may be measured by culturing target cells on macrophage monolayers and determining target cell survival by either Rb or H-thymidine incorporation. Using these techniques, one can demonstrate cytotoxicity of nonimmune human and rat macrophages to a variety of tumor cell lines. Toxicity becomes maximal 8 to 16 hr after attaching macrophages to plastic surfaces and disappears after 36 to 48 hr. Addition of Escherichia coli endotoxin at 48 hr restores macrophage toxicity. Culture of macrophages on plastic surfaces also results in the release, nonspecifically, of a soluble cytotoxin whose kinetics of release, disappearance, and reappearance after endotoxin all parallel the M-CMC. The amount of toxin released into cultures containing target cells is markedly reduced, suggesting that it is the mediator of the M-CMC and that it is utilized or absorbed during target destruction. Sephadex G-100 chromatography and treatment with heterologous anti-lymphotoxin can distinguish this toxin from lymphotoxin."}
{"id": "PMID:1097514", "title": "Genetic control of susceptibility to experimental allergic encephalomyelitis and the Ag-B locus of rats.", "content": "Injection of guinea pig spinal cord with complete Freund's adjuvant induces EAE in Lewis (Ag-B1) and DA (Ag-B4) rats, but not in rats of the BN (Ag-B3) strain. When BN is crossed with Lewis, susceptibility is determined by a gene linked to the Ag-B histocompatibility locus, but a much more complex mode of inheritance is involved when BN and DA rats are crossed. Six inbred strains possessing the Ag-B1 histocompatibility allele have been found to be susceptible to EAE. Evidence for recombination between the Ag-B locus and the gene determining susceptibility to EAE has not yet been obtained.", "contents": "Genetic control of susceptibility to experimental allergic encephalomyelitis and the Ag-B locus of rats. Injection of guinea pig spinal cord with complete Freund's adjuvant induces EAE in Lewis (Ag-B1) and DA (Ag-B4) rats, but not in rats of the BN (Ag-B3) strain. When BN is crossed with Lewis, susceptibility is determined by a gene linked to the Ag-B histocompatibility locus, but a much more complex mode of inheritance is involved when BN and DA rats are crossed. Six inbred strains possessing the Ag-B1 histocompatibility allele have been found to be susceptible to EAE. Evidence for recombination between the Ag-B locus and the gene determining susceptibility to EAE has not yet been obtained."}
{"id": "PMID:1097515", "title": "Class differences in the antibody response of HLC and LLC mice to sheep red blood cells.", "content": "The immune response to sheep red blood cells (SRBC) has been studied in two mouse lines selectively bred for a difference in leukocyte levels. Low leukocyte count (LLC) mice responded to multiple injections of 4 x 10(7) SRBC with a predominant IgM response whereas high leukocyte count (HLC) mice produced IgG almost exclusively, even after a single injection of SRBC. F1 mice responded like the LLC parent and backcross data implied genetic control at multiple loci. No correlation was found between the class of antibody produced in response to SRBC and the leukocyte count in backcross mice. Large numbers of plaque-forming cells (PFC) were detected in the spleens of HLC mice relative to the number detected in LLC spleens after primary and secondary immunizations with SRBC.", "contents": "Class differences in the antibody response of HLC and LLC mice to sheep red blood cells. The immune response to sheep red blood cells (SRBC) has been studied in two mouse lines selectively bred for a difference in leukocyte levels. Low leukocyte count (LLC) mice responded to multiple injections of 4 x 10(7) SRBC with a predominant IgM response whereas high leukocyte count (HLC) mice produced IgG almost exclusively, even after a single injection of SRBC. F1 mice responded like the LLC parent and backcross data implied genetic control at multiple loci. No correlation was found between the class of antibody produced in response to SRBC and the leukocyte count in backcross mice. Large numbers of plaque-forming cells (PFC) were detected in the spleens of HLC mice relative to the number detected in LLC spleens after primary and secondary immunizations with SRBC."}
{"id": "PMID:1097516", "title": "T cell activation and cellular cooperation in autoimmune NZB/NZW F hybrid mice.", "content": "Old (6 months) overtly autoimmune female NZB X NZW F1 (B/W) mice were markedly hyporesponsive to sheep erythrocytes (SRBC). The response to SRBC was restored by a) simultaneously injecting lipopolysaccharide (LPS) with SRBC or b) transferring bone marrow and thymocytes with SRBC into lethally x-irradiated (100 R) syngeneic old recipients. The in vitro PFC response of old B/W spleen cells to SRBC was restored by adding in culture a) theta-positive and radioresistant spleen cells from old B/W mice primed with homologous antigen or b) activated T cells from the spleens of lethally x-irradiated (1000 R) old B/W mice injected with old syngeneic thymocytes and SRBC but not horse erythrocytes. Various populations of unprimed lymphoid cells from young (4 to 6 weeks) female B/W mice, which respond normally to SRBC, were not capable of restoring the response of old syngeneic mice in vitro or in vivo. These results suggest the existence of a suppressor of T cell activation and/or B and T cell interaction in old autoimmune B/W mice.", "contents": "T cell activation and cellular cooperation in autoimmune NZB/NZW F hybrid mice. Old (6 months) overtly autoimmune female NZB X NZW F1 (B/W) mice were markedly hyporesponsive to sheep erythrocytes (SRBC). The response to SRBC was restored by a) simultaneously injecting lipopolysaccharide (LPS) with SRBC or b) transferring bone marrow and thymocytes with SRBC into lethally x-irradiated (100 R) syngeneic old recipients. The in vitro PFC response of old B/W spleen cells to SRBC was restored by adding in culture a) theta-positive and radioresistant spleen cells from old B/W mice primed with homologous antigen or b) activated T cells from the spleens of lethally x-irradiated (1000 R) old B/W mice injected with old syngeneic thymocytes and SRBC but not horse erythrocytes. Various populations of unprimed lymphoid cells from young (4 to 6 weeks) female B/W mice, which respond normally to SRBC, were not capable of restoring the response of old syngeneic mice in vitro or in vivo. These results suggest the existence of a suppressor of T cell activation and/or B and T cell interaction in old autoimmune B/W mice."}
{"id": "PMID:1097517", "title": "Inhibition of in vitro lymphokine synthesis by glucocorticosteroids.", "content": "At pharmacological levels, glucocorticosteroids inhibited two antigen-induced lymphocyte functions, in vitro proliferation and lymphokine synthesis. Lymphocyte production of both macrophage chemotactic factor (CTX) and macrophage inhibition factor (MIF) were decreased in the presence of hydrocortisone. The corticosteroid also blocked the action of MIF on macrophages but did not interfere with the action of CTX on macrophages. Thus, steroids can suppress the immune response at two different stages: by blocking lymphocyte activation and mediator synthesis and also by interfering with the interaction of certain effector molecules with their target cells. Furthermore, these findings suggested that MIF and CTX have distinct mechanisms of action on the effector macrophages, one being sensitive and one resistant to steroids.", "contents": "Inhibition of in vitro lymphokine synthesis by glucocorticosteroids. At pharmacological levels, glucocorticosteroids inhibited two antigen-induced lymphocyte functions, in vitro proliferation and lymphokine synthesis. Lymphocyte production of both macrophage chemotactic factor (CTX) and macrophage inhibition factor (MIF) were decreased in the presence of hydrocortisone. The corticosteroid also blocked the action of MIF on macrophages but did not interfere with the action of CTX on macrophages. Thus, steroids can suppress the immune response at two different stages: by blocking lymphocyte activation and mediator synthesis and also by interfering with the interaction of certain effector molecules with their target cells. Furthermore, these findings suggested that MIF and CTX have distinct mechanisms of action on the effector macrophages, one being sensitive and one resistant to steroids."}
{"id": "PMID:1097518", "title": "Antigenic selection of IgG precursor subpopulations.", "content": "The temporal increase in the antigen-binding characteristic of the IgG antibody synthesized after primary immunization is thought to result from antigen preferentially selecting those precursors of the antibody-forming cells with higher affinity receptors. We have investigated this regulatory role of antigen on the selection and expression of IgG precursor subpopulations in rabbit lymph node populations undergoing the secondary anti-bovine serum albumin (BSA) response in vitro. The data indicate that although immunologic memory increased between 1 and 4 months after immunization there was not an increase in antigen sensitivity. We also determined the avidity of the IgG antibody secreted by PFC elicited in the cell cultures. Although the avidity of antibody secreted by PFC elicited with low concentrations of antigen was 2-fold greater than when high concentrations of antigen were employed, analyses of the avidity subpopulations of IgG PFC revealed that memory potential was not restricted to high affinity-primed cells. Moreover, populations of PFC that gave differing avidity values were similar in terms of their plaque diameters and IgG secretion rates.", "contents": "Antigenic selection of IgG precursor subpopulations. The temporal increase in the antigen-binding characteristic of the IgG antibody synthesized after primary immunization is thought to result from antigen preferentially selecting those precursors of the antibody-forming cells with higher affinity receptors. We have investigated this regulatory role of antigen on the selection and expression of IgG precursor subpopulations in rabbit lymph node populations undergoing the secondary anti-bovine serum albumin (BSA) response in vitro. The data indicate that although immunologic memory increased between 1 and 4 months after immunization there was not an increase in antigen sensitivity. We also determined the avidity of the IgG antibody secreted by PFC elicited in the cell cultures. Although the avidity of antibody secreted by PFC elicited with low concentrations of antigen was 2-fold greater than when high concentrations of antigen were employed, analyses of the avidity subpopulations of IgG PFC revealed that memory potential was not restricted to high affinity-primed cells. Moreover, populations of PFC that gave differing avidity values were similar in terms of their plaque diameters and IgG secretion rates."}
{"id": "PMID:1097519", "title": "The enhancing effect of mitogens on the in vivo immune response of rabbits.", "content": "The effect of Con A and PHA on the primary humoral immune response in rabbits was investigated and compared with the effect of LPS and irradiation. Both Con A and PHA enhanced PFC levels and precipitating antibody titers to HuIgG when injected simultaneously with AHuIgG. Comparable enhancement of the response to HuIgG was obtained by simultaneous injection of LPS with AHuIgG or by irradiating rabbits (500 R) 24 hr after injection of the antigen. Further studies with Con A showed that its adjuvant effect was not restricted to a particular antigen since it also enhanced antibody responses to both SRBC and Bov Tg when injected simultaneously with either of these antigens. Although optimal enhancement of the response to HuIgG occurred when antigen and mitogen were injected simultaneously, some enhancement was obtained if Con A was injected 2 days after antigen, whereas the response was decreased if Con A was injected 2 days before antigen. Splenic PFC in A/J mice injected with doses of AHuIgG and Con A equivalent on a body weight basis to those used in rabbits were also enhanced by simultaneous injection of Con A with the antigen.", "contents": "The enhancing effect of mitogens on the in vivo immune response of rabbits. The effect of Con A and PHA on the primary humoral immune response in rabbits was investigated and compared with the effect of LPS and irradiation. Both Con A and PHA enhanced PFC levels and precipitating antibody titers to HuIgG when injected simultaneously with AHuIgG. Comparable enhancement of the response to HuIgG was obtained by simultaneous injection of LPS with AHuIgG or by irradiating rabbits (500 R) 24 hr after injection of the antigen. Further studies with Con A showed that its adjuvant effect was not restricted to a particular antigen since it also enhanced antibody responses to both SRBC and Bov Tg when injected simultaneously with either of these antigens. Although optimal enhancement of the response to HuIgG occurred when antigen and mitogen were injected simultaneously, some enhancement was obtained if Con A was injected 2 days after antigen, whereas the response was decreased if Con A was injected 2 days before antigen. Splenic PFC in A/J mice injected with doses of AHuIgG and Con A equivalent on a body weight basis to those used in rabbits were also enhanced by simultaneous injection of Con A with the antigen."}
{"id": "PMID:1097520", "title": "The effect of staphylococcal enterotoxins on the primary in vitro immune response.", "content": "Staphylococcal enterotoxins, like several plant lectins, have been shown to stimulate mitogenic activity and lymphokine production in lymphocytes. The effect of enterotoxins A and B on the primary in vitro plaque-forming cell (PFC) response of mouse (C57BL/6) spleen cells to sheep red blood cells (SRBC) was examined. PFC responses in 4- or 5-day cultures were inhibited greater than 90% by 0.1 mug enterotoxin A and by 3- to 6-mug enterotoxin B when the toxins were added to the cultures either at the time of SRBC addition or 24 hr before. Both enterotoxins A and B were shown to be T lymphocyte mitogens. Kinetic data indicated that the enterotoxins (10 mug) inhibited the PFC response as early as day 3, but were relatively more inhibitory at days 4 and 5. The PFC response was inhibited when enterotoxins were added to cultures at the same time as antigen or a day later; it was enhanced when added at day 2; and it was unaffected when added at day 3 of 4-day cultures. Further, the presence of enterotoxin A during the first 24 hr of culture and subsequent removal was still as effective in inhibiting the PFC response as when it was present throughout the culture period. The PFC inhibitory properties of enterotoxins appear, then, to affect some early event(s) in the in vitro immune response. Alpha-methyl-D-mannopyranoside blocked the PFC inhibitory effect of concanavalin A (con A), but it had no effect on enterotoxin A. The two mitogens appear, then, to react with different receptors on the lymphocytes or to differ in the dynamics of their reactivity. The effects of staphylococcal enterotoxins on the PFC response of spleen cells were remarkably similar to those reported for lectins such as con A. Enterotoxins are structurally simpler than con A and should therefore be quite useful in studying various biologic activities of lymphocytes.", "contents": "The effect of staphylococcal enterotoxins on the primary in vitro immune response. Staphylococcal enterotoxins, like several plant lectins, have been shown to stimulate mitogenic activity and lymphokine production in lymphocytes. The effect of enterotoxins A and B on the primary in vitro plaque-forming cell (PFC) response of mouse (C57BL/6) spleen cells to sheep red blood cells (SRBC) was examined. PFC responses in 4- or 5-day cultures were inhibited greater than 90% by 0.1 mug enterotoxin A and by 3- to 6-mug enterotoxin B when the toxins were added to the cultures either at the time of SRBC addition or 24 hr before. Both enterotoxins A and B were shown to be T lymphocyte mitogens. Kinetic data indicated that the enterotoxins (10 mug) inhibited the PFC response as early as day 3, but were relatively more inhibitory at days 4 and 5. The PFC response was inhibited when enterotoxins were added to cultures at the same time as antigen or a day later; it was enhanced when added at day 2; and it was unaffected when added at day 3 of 4-day cultures. Further, the presence of enterotoxin A during the first 24 hr of culture and subsequent removal was still as effective in inhibiting the PFC response as when it was present throughout the culture period. The PFC inhibitory properties of enterotoxins appear, then, to affect some early event(s) in the in vitro immune response. Alpha-methyl-D-mannopyranoside blocked the PFC inhibitory effect of concanavalin A (con A), but it had no effect on enterotoxin A. The two mitogens appear, then, to react with different receptors on the lymphocytes or to differ in the dynamics of their reactivity. The effects of staphylococcal enterotoxins on the PFC response of spleen cells were remarkably similar to those reported for lectins such as con A. Enterotoxins are structurally simpler than con A and should therefore be quite useful in studying various biologic activities of lymphocytes."}
{"id": "PMID:1097521", "title": "Ia alloantigen(s) detected on thymocytes by use of a fluorescence-activated cell sorter.", "content": "With the increased sensitivity of detection of fluorescence made possible by a fluorescence-activated cell sorter (FACS), Ia antigen(s) have been demonstrated, although very small amounts, on murine thymocytes by using an indirect immunofluorescent technique. Absorption studies indicate that the Ia antigens detected on thymocytes are also present on spleen B cells, however, they do not rule out the possibility that certain Ia antigens may exist only on B cells. These studies suggest that the surface distribution and/or the decreased density of Ia antigens on thymocytes relative to peripheral B cells may preclude their demonstration by more conventional assays. Although these studies demonstrate Ia antigens on less than 50% of thymocytes, the percentage may in fact be much higher since current methods do not allow an upper limit to be assigned.", "contents": "Ia alloantigen(s) detected on thymocytes by use of a fluorescence-activated cell sorter. With the increased sensitivity of detection of fluorescence made possible by a fluorescence-activated cell sorter (FACS), Ia antigen(s) have been demonstrated, although very small amounts, on murine thymocytes by using an indirect immunofluorescent technique. Absorption studies indicate that the Ia antigens detected on thymocytes are also present on spleen B cells, however, they do not rule out the possibility that certain Ia antigens may exist only on B cells. These studies suggest that the surface distribution and/or the decreased density of Ia antigens on thymocytes relative to peripheral B cells may preclude their demonstration by more conventional assays. Although these studies demonstrate Ia antigens on less than 50% of thymocytes, the percentage may in fact be much higher since current methods do not allow an upper limit to be assigned."}
{"id": "PMID:1097523", "title": "Antigenic competition between two sequentially acting antigens. Immunosuppressive effect of T cells in spleen and lymph nodes of mouse.", "content": "This study concerns the antigenic competition among heterologous red cells, a T suppressor-dependent phenomenon, in three cases, when the two antigens are administered by various routes, in (a) splenectomized mice or in Nude mice reconstituted with (b) lymph node cells, or spleen cells. Competition occurs whatever the routes of antigenic introduction, except when the two antigens are injected subcutaneously in the same leg and the anti-SRC plaque-forming cells are counted in the draining lymph nodes. In that case, the first antigen plays the role of adjuvant to the second. An adjuvant effect is also observed in splenectomized mice when the antigens are introduced intraperitoneally. However, competition does not occur in Nude mice but is present when those animals have been reconstituted either with lymphnode cells or with spleen cells. Those results play in favor of the presence of T suppressors, both in lymph nodes and in spleen; however, in lymph nodes, the immunosuppressive action induced by the first antigen is exceeded by an concomitant adjuvant effect.", "contents": "Antigenic competition between two sequentially acting antigens. Immunosuppressive effect of T cells in spleen and lymph nodes of mouse. This study concerns the antigenic competition among heterologous red cells, a T suppressor-dependent phenomenon, in three cases, when the two antigens are administered by various routes, in (a) splenectomized mice or in Nude mice reconstituted with (b) lymph node cells, or spleen cells. Competition occurs whatever the routes of antigenic introduction, except when the two antigens are injected subcutaneously in the same leg and the anti-SRC plaque-forming cells are counted in the draining lymph nodes. In that case, the first antigen plays the role of adjuvant to the second. An adjuvant effect is also observed in splenectomized mice when the antigens are introduced intraperitoneally. However, competition does not occur in Nude mice but is present when those animals have been reconstituted either with lymphnode cells or with spleen cells. Those results play in favor of the presence of T suppressors, both in lymph nodes and in spleen; however, in lymph nodes, the immunosuppressive action induced by the first antigen is exceeded by an concomitant adjuvant effect."}
{"id": "PMID:1097524", "title": "Separation of lymphocytes by specific adherence to cellular monolayers containing protein A of Staphylococcus aureus.", "content": "A technique was developed for the isolation of lymphocytes according to their surface antigenic markers. The method was based on the general reaction of protein A (SpA from S. aureus) with the Fc-part of an IgG antibody. Monolayers of S. aureus or Spa-coated sheep red blood cells (SpA-SRBC) fix antibody-charged cells specifically; non-fixed cells are easily removed by washing. Alternatively, the monolayers can be treated with a cell surface specific antibody prior to addition of non-charged cells. Monolayer-adhered cells were detached by the addition of lysostaphin (bacterial monolayer) or ammonium chloride (SpA-SRBC monolayer). As an example, Ig-bearing cells were isolated from mouse spleen lymphocytes using a specific rabbit anti-mouse Ig serum for charging either the cells or the monolayers. The recovery of Ig-bearing cells was approximately 84%. The purity of the cells was approximately 83% and the viability 89%. Antibody-SpA complexes on the surface of isolated cells were removed either by trypsin treatment or by cultivation. Ig on the cell surface is restored after 16 hr of cultivation.", "contents": "Separation of lymphocytes by specific adherence to cellular monolayers containing protein A of Staphylococcus aureus. A technique was developed for the isolation of lymphocytes according to their surface antigenic markers. The method was based on the general reaction of protein A (SpA from S. aureus) with the Fc-part of an IgG antibody. Monolayers of S. aureus or Spa-coated sheep red blood cells (SpA-SRBC) fix antibody-charged cells specifically; non-fixed cells are easily removed by washing. Alternatively, the monolayers can be treated with a cell surface specific antibody prior to addition of non-charged cells. Monolayer-adhered cells were detached by the addition of lysostaphin (bacterial monolayer) or ammonium chloride (SpA-SRBC monolayer). As an example, Ig-bearing cells were isolated from mouse spleen lymphocytes using a specific rabbit anti-mouse Ig serum for charging either the cells or the monolayers. The recovery of Ig-bearing cells was approximately 84%. The purity of the cells was approximately 83% and the viability 89%. Antibody-SpA complexes on the surface of isolated cells were removed either by trypsin treatment or by cultivation. Ig on the cell surface is restored after 16 hr of cultivation."}
{"id": "PMID:1097525", "title": "B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes.", "content": "Cultured cells from mouse thoracic duct, spleen, lymph node, Peyer's patches, and bone marrow gave rise to cells containing large amounts of cytoplasmic IgM, IgGl, and IgG2 when stimulated by bacterial lipopolysaccharide (LPS). Differntiation of IgA producers occurred in bone marrow only, and to a lesser extent than other classes. Significant IgM responses preceded development of cells containing other classes. The differentiation of IgG and IgA producers did not appear to depend on T cells, since cultures from nu/nu or thymectomized-irradiated, bone marrow-protected mice responded as well as normals. Cultures from mice rendered deficient in B cells by anti-mu treatment responded normally to T cell mitogens, but did not proliferate or give rise to immunoglobulin-secreting cells when stimulated with LPS. Bone marrow cultures gave relatively meager proliferative responses to LPS, but generated as many or more immunoglobulin-secreting cells as did other tissues.", "contents": "B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes. Cultured cells from mouse thoracic duct, spleen, lymph node, Peyer's patches, and bone marrow gave rise to cells containing large amounts of cytoplasmic IgM, IgGl, and IgG2 when stimulated by bacterial lipopolysaccharide (LPS). Differntiation of IgA producers occurred in bone marrow only, and to a lesser extent than other classes. Significant IgM responses preceded development of cells containing other classes. The differentiation of IgG and IgA producers did not appear to depend on T cells, since cultures from nu/nu or thymectomized-irradiated, bone marrow-protected mice responded as well as normals. Cultures from mice rendered deficient in B cells by anti-mu treatment responded normally to T cell mitogens, but did not proliferate or give rise to immunoglobulin-secreting cells when stimulated with LPS. Bone marrow cultures gave relatively meager proliferative responses to LPS, but generated as many or more immunoglobulin-secreting cells as did other tissues."}
{"id": "PMID:1097526", "title": "Sera and the in vitro induction of immune responses. I. Bacterial contamination and the generation of good fetal bovine sera.", "content": "More than 200 samples of pooled fetal bovine sera (FBS) were tested to determine their capacity to support primary immune responses by cultured mouse spleen cells. Less than 10% of the FBS samples were fully supportive; the majority of the samples were moderately to very deficient. Several lines of evidence suggest that bacterial contamination during the processing of sera plays a major role in determining which sera are supportive. Serum samples known to have been temporarily contamined during processing were strongly supportive. Samples of FBS which were likely never to have been contaminated were deficient. Bacteria from a specific lot of supportive serum converted a very deficient serum into one which was supportive. Several mechanicsms by which bacteria could effect sera are discussed.", "contents": "Sera and the in vitro induction of immune responses. I. Bacterial contamination and the generation of good fetal bovine sera. More than 200 samples of pooled fetal bovine sera (FBS) were tested to determine their capacity to support primary immune responses by cultured mouse spleen cells. Less than 10% of the FBS samples were fully supportive; the majority of the samples were moderately to very deficient. Several lines of evidence suggest that bacterial contamination during the processing of sera plays a major role in determining which sera are supportive. Serum samples known to have been temporarily contamined during processing were strongly supportive. Samples of FBS which were likely never to have been contaminated were deficient. Bacteria from a specific lot of supportive serum converted a very deficient serum into one which was supportive. Several mechanicsms by which bacteria could effect sera are discussed."}
{"id": "PMID:1097527", "title": "Sera and the in vitro induction of immune responses. II. Inhibitory effects of newborn calf sera on primary humoral responses.", "content": "The effect of newborn calf (NBC) sera on the in vitro generation of immune responses were studied. NBC sera inhibited the generation of primary humoral responses to SRBC. The inhibitory activity was dose-dependent and diluted out rapidly. Adding inhibitory doses of NBC serum to test cultures at zero or 24 hr resulted in approximately the same amount of inhibition. Pulsing experiments showed that the test cultures were most sensitive to the inhibitory effects of NBC serum during the 24- to 48-hr period. In contrast to its inhibitory effects on primary humoral responses, NBC sera supported the in vitro generation of secondary humoral responses and significantly increased the responses of some of these over control levels. We found that sera obtained from newborn calves before nursing were not inhibitory for primary responses but sera obtained from the same calves 7 days later did inhibit these responses. We postulate that the inhibitory effects of NBC sera may represent a physiologic control mechanism which becomes active when immunologic maturity develops and that it possibly involves the inactivation of cell mediators.", "contents": "Sera and the in vitro induction of immune responses. II. Inhibitory effects of newborn calf sera on primary humoral responses. The effect of newborn calf (NBC) sera on the in vitro generation of immune responses were studied. NBC sera inhibited the generation of primary humoral responses to SRBC. The inhibitory activity was dose-dependent and diluted out rapidly. Adding inhibitory doses of NBC serum to test cultures at zero or 24 hr resulted in approximately the same amount of inhibition. Pulsing experiments showed that the test cultures were most sensitive to the inhibitory effects of NBC serum during the 24- to 48-hr period. In contrast to its inhibitory effects on primary humoral responses, NBC sera supported the in vitro generation of secondary humoral responses and significantly increased the responses of some of these over control levels. We found that sera obtained from newborn calves before nursing were not inhibitory for primary responses but sera obtained from the same calves 7 days later did inhibit these responses. We postulate that the inhibitory effects of NBC sera may represent a physiologic control mechanism which becomes active when immunologic maturity develops and that it possibly involves the inactivation of cell mediators."}
{"id": "PMID:1097528", "title": "Studies on the human T lymphocyte population. III. Synthesis and release of the lymphocyte receptor for sheep red blood cells by stimulated human T lymphoblasts.", "content": "The IgG fraction of a goat antibody specific for human T lymphocytes (ATG) and that of a rabbit antibody directed (anti-Ts) were used to study the synthesis, display, and release of the receptor for SRBC (Ts) by normal human peripheral lymphocytes. A large percentage of lymphoblasts obtained by stimulation of lymphocytes with PHA or PWM formed multilayered rosettes with SRBC. The vast majority of these rosetting lymphoblasts were T lymphocyte derived, whereas few B lymphoblasts formed rosettes. Significant increases were found in the quantity of Ts on the lymphoblast membrane and in the supernatants from PHA-stimulated cultures, especially late in transformation. Furthermore, lymphocytes cultured with PHA and 3H-leucine synthesized and released radiolabeled Ts.", "contents": "Studies on the human T lymphocyte population. III. Synthesis and release of the lymphocyte receptor for sheep red blood cells by stimulated human T lymphoblasts. The IgG fraction of a goat antibody specific for human T lymphocytes (ATG) and that of a rabbit antibody directed (anti-Ts) were used to study the synthesis, display, and release of the receptor for SRBC (Ts) by normal human peripheral lymphocytes. A large percentage of lymphoblasts obtained by stimulation of lymphocytes with PHA or PWM formed multilayered rosettes with SRBC. The vast majority of these rosetting lymphoblasts were T lymphocyte derived, whereas few B lymphoblasts formed rosettes. Significant increases were found in the quantity of Ts on the lymphoblast membrane and in the supernatants from PHA-stimulated cultures, especially late in transformation. Furthermore, lymphocytes cultured with PHA and 3H-leucine synthesized and released radiolabeled Ts."}
{"id": "PMID:1097529", "title": "Studies on lymphokines: the production of antibody to guinea pig lymphotoxin and its use to distinguish lymphotoxin from migration inhibitory factor and mitogenic factor.", "content": "Guinea pig lymphotoxin, which was produced by stimulating immune lymph node cells with the specific antigen, ovalbumin, was partially purified by the sequential use of Pevikon block electrophoresis, ion exchange chromatography on DEAE-cellulose, and polyacrylamide disc gel electrophoresis. The lymphotoxin recovered from the final step was purified approximately 200- to 300-fold as compared to the starting material, but nevertheless contained at least one major contaminant protein. Rabbits were immunized with the partially purified lymphotoxin, and an antiserum capable of neutralizing the cytotoxic effect of lymphotoxin was obtained. The anti-lymphotoxin activity was shown to be due predominantly to IgM immunoglobulin. The antiserum neutralized concanavalin A-induced lymphotoxin with equal efficiency as antigen-induced lymphotoxin. On the other hand, the anti-lymphotoxin serum did not neutralize preformed mitogenic factor or migration inhibitory factor (MIF) which had been produced by antigen-stimulated immune lymph node cells. Thus these activities appear to be due to substances which are antigenically distinct from lymphotoxin. Likewise, anti-lymphotoxin serum, when present in cultures of antigen-stimulated immune lymphoid cells, caused no diminution in the amount of mitogenic factor or MIF which was produced, indicating that it had no direct deleterious effect on the function of the lymphoid cells responsible for producing these lymphokines.", "contents": "Studies on lymphokines: the production of antibody to guinea pig lymphotoxin and its use to distinguish lymphotoxin from migration inhibitory factor and mitogenic factor. Guinea pig lymphotoxin, which was produced by stimulating immune lymph node cells with the specific antigen, ovalbumin, was partially purified by the sequential use of Pevikon block electrophoresis, ion exchange chromatography on DEAE-cellulose, and polyacrylamide disc gel electrophoresis. The lymphotoxin recovered from the final step was purified approximately 200- to 300-fold as compared to the starting material, but nevertheless contained at least one major contaminant protein. Rabbits were immunized with the partially purified lymphotoxin, and an antiserum capable of neutralizing the cytotoxic effect of lymphotoxin was obtained. The anti-lymphotoxin activity was shown to be due predominantly to IgM immunoglobulin. The antiserum neutralized concanavalin A-induced lymphotoxin with equal efficiency as antigen-induced lymphotoxin. On the other hand, the anti-lymphotoxin serum did not neutralize preformed mitogenic factor or migration inhibitory factor (MIF) which had been produced by antigen-stimulated immune lymph node cells. Thus these activities appear to be due to substances which are antigenically distinct from lymphotoxin. Likewise, anti-lymphotoxin serum, when present in cultures of antigen-stimulated immune lymphoid cells, caused no diminution in the amount of mitogenic factor or MIF which was produced, indicating that it had no direct deleterious effect on the function of the lymphoid cells responsible for producing these lymphokines."}
{"id": "PMID:1097530", "title": "Modulation of the immune response by antigen-reactive lymphocytes after cultivation with gangliosides.", "content": "GM1 ganglioside isolated from mouse brain was found to be capable of inhibiting the thymocytotoxicity of AKR anti-Thy 1.2 antiserum and to a lesser extent anti-BAtheta antiserum. GD1b ganglioside of mouse brain and thymocytes inhibited the thymocytotoxicity of rabbit anti-BAtheta anti-serum for mouse thymocytes. When GM1 ganglioside, in the form of cholesterol-lecithin liposomes, was incubated with spleen cell cultures in the presence of SRBC, depressed anti-SRBC hemolytic plaque responses were observed. This effect could be neutralized by first absorbing the GM1 liposomes with anti-Thy-1.2 antibodies. The kinetics of GM1 numbers when the ganglioside was added even as late as the 4th day of culture. Results of experiments in which GM1 ganglioside was incubated with either bone marrow cells or with thymocytes suggest that the target cell for GM1 liposomes is the B lymphocytes.", "contents": "Modulation of the immune response by antigen-reactive lymphocytes after cultivation with gangliosides. GM1 ganglioside isolated from mouse brain was found to be capable of inhibiting the thymocytotoxicity of AKR anti-Thy 1.2 antiserum and to a lesser extent anti-BAtheta antiserum. GD1b ganglioside of mouse brain and thymocytes inhibited the thymocytotoxicity of rabbit anti-BAtheta anti-serum for mouse thymocytes. When GM1 ganglioside, in the form of cholesterol-lecithin liposomes, was incubated with spleen cell cultures in the presence of SRBC, depressed anti-SRBC hemolytic plaque responses were observed. This effect could be neutralized by first absorbing the GM1 liposomes with anti-Thy-1.2 antibodies. The kinetics of GM1 numbers when the ganglioside was added even as late as the 4th day of culture. Results of experiments in which GM1 ganglioside was incubated with either bone marrow cells or with thymocytes suggest that the target cell for GM1 liposomes is the B lymphocytes."}
{"id": "PMID:1097531", "title": "Differentiating feline T and B lymphocytes by rosette formation.", "content": "The number of bone marrow-derived and thymus-dependent lymphocytes in the peripheral blood of felines was assessed. Feline bone marrow-derived lymphocytes were determined by erythrocyte antibody complement (EAC)-rosette formation and membrane immunofluorescence. A good correlation was observed between the cells forming EAC-rosettes (mean of 40.2 +/- 9.7%) and those possessing membrane immunoglobulins (mean of 45.3 +/- 4.1%). Feline thymus-dependent lymphocytes form non-immune (E)-rosettes with rodent erythrocytes (mouse, rat, guinea pig) but not with erythrocytes from humans, monkeys, dogs, mongooses, chickens, rabbits, sheep, and hamsters. An average of 32.3 +/- 992% of the peripheral blood lymphobytes of cats formed rosettes with guinea pig erythrocytes. Antibody blocking, cytotoxicity, and mix-rosette studies demonstrated that guinea pig red blood cells bind specifically to T lymphocytes. Of feline peripheral blood lymphocytes, 41.0 +/- 7.5% showed fluorescent staining with fluorescein-labeled rabbit anti-cat thymus antiserum.", "contents": "Differentiating feline T and B lymphocytes by rosette formation. The number of bone marrow-derived and thymus-dependent lymphocytes in the peripheral blood of felines was assessed. Feline bone marrow-derived lymphocytes were determined by erythrocyte antibody complement (EAC)-rosette formation and membrane immunofluorescence. A good correlation was observed between the cells forming EAC-rosettes (mean of 40.2 +/- 9.7%) and those possessing membrane immunoglobulins (mean of 45.3 +/- 4.1%). Feline thymus-dependent lymphocytes form non-immune (E)-rosettes with rodent erythrocytes (mouse, rat, guinea pig) but not with erythrocytes from humans, monkeys, dogs, mongooses, chickens, rabbits, sheep, and hamsters. An average of 32.3 +/- 992% of the peripheral blood lymphobytes of cats formed rosettes with guinea pig erythrocytes. Antibody blocking, cytotoxicity, and mix-rosette studies demonstrated that guinea pig red blood cells bind specifically to T lymphocytes. Of feline peripheral blood lymphocytes, 41.0 +/- 7.5% showed fluorescent staining with fluorescein-labeled rabbit anti-cat thymus antiserum."}
{"id": "PMID:1097532", "title": "Evidence that uridine incorporation is not a selective marker for mouse lymphocyte subclasses.", "content": "We demonstrate here that mouse T+ and Ig+ lymphocytes do not show the differential incorporation of 3H-uridine into acid precipitable cellular components described for these lymphocyte subpopulation in the rat.", "contents": "Evidence that uridine incorporation is not a selective marker for mouse lymphocyte subclasses. We demonstrate here that mouse T+ and Ig+ lymphocytes do not show the differential incorporation of 3H-uridine into acid precipitable cellular components described for these lymphocyte subpopulation in the rat."}
{"id": "PMID:1097533", "title": "Investigation of the recovery phenomenon in immunofluorescence after laser excitation.", "content": "Laser excitation was applied in two standard immunopathological indirect immunoflorescence systems in order to investigate bleaching and recovery of fluorescence intensity upon repeated illuminations. Variation of illumination times and dark periods between these showed that the recovery phenomenon is a function of both the (time times energy) product of excitation and the length of dark intervals. The value of high energy excitation in immunofluorescence in providing insight into the mechanisms of bleaching and recovery is discussed.", "contents": "Investigation of the recovery phenomenon in immunofluorescence after laser excitation. Laser excitation was applied in two standard immunopathological indirect immunoflorescence systems in order to investigate bleaching and recovery of fluorescence intensity upon repeated illuminations. Variation of illumination times and dark periods between these showed that the recovery phenomenon is a function of both the (time times energy) product of excitation and the length of dark intervals. The value of high energy excitation in immunofluorescence in providing insight into the mechanisms of bleaching and recovery is discussed."}
{"id": "PMID:1097540", "title": "Cutaneous immunopathology: recent observations.", "content": "Immunofluorescent studies are currently being done on patients with pemphigus, pemphigoid, dermatitis, lupus erythematosus and its variants, the cutaneous prophyrias, scarring alopecia, erosive mouth lesions, light-sensitive disorders, and cutaneous vasculitis. In this paper I shall review some of the recent advances in immunopathology and report the results that have been obtained in our laboratory.", "contents": "Cutaneous immunopathology: recent observations. Immunofluorescent studies are currently being done on patients with pemphigus, pemphigoid, dermatitis, lupus erythematosus and its variants, the cutaneous prophyrias, scarring alopecia, erosive mouth lesions, light-sensitive disorders, and cutaneous vasculitis. In this paper I shall review some of the recent advances in immunopathology and report the results that have been obtained in our laboratory."}
{"id": "PMID:1097541", "title": "The significance of cutaneous immunoglobulin deposits in lupus erythematosus and NZB/NZW F1 hybrid mice.", "content": "In 50 to 60 percent of patients with systemic lupus erythematosus (SLE), a band of immunoglobulins beneath the epidermis of visibly normal skin. This highly specific finding has been used as a diagnostic test (Lupus Band Test) for SLE. Similar immunoglobulin deposits are found in an inbred strain of New Zealand mice which spontaneously develop an autoimmune disease with many features of SLE. Subepidermal immunoglobulin deposits are found most frequently in SLE patients with proliferative glomerulonephritis, hypocomplementemia, and serum antibodies to native DNA (anti-nDNA). When anti-nDNA levels are suppressed by cyclophosphamide, these deposits disappear. The subepidermal accumulation of immunoglobulin in SLE patients and in the mouse model apparently depends on the presence of antibody to native DNA. It is proposed that serum anti-nDNA precipitates with nDNA which is released locally from epidermal nuclear breakdown.", "contents": "The significance of cutaneous immunoglobulin deposits in lupus erythematosus and NZB/NZW F1 hybrid mice. In 50 to 60 percent of patients with systemic lupus erythematosus (SLE), a band of immunoglobulins beneath the epidermis of visibly normal skin. This highly specific finding has been used as a diagnostic test (Lupus Band Test) for SLE. Similar immunoglobulin deposits are found in an inbred strain of New Zealand mice which spontaneously develop an autoimmune disease with many features of SLE. Subepidermal immunoglobulin deposits are found most frequently in SLE patients with proliferative glomerulonephritis, hypocomplementemia, and serum antibodies to native DNA (anti-nDNA). When anti-nDNA levels are suppressed by cyclophosphamide, these deposits disappear. The subepidermal accumulation of immunoglobulin in SLE patients and in the mouse model apparently depends on the presence of antibody to native DNA. It is proposed that serum anti-nDNA precipitates with nDNA which is released locally from epidermal nuclear breakdown."}
{"id": "PMID:1097542", "title": "The epidermal-dermal junction.", "content": "Ultrastructurally, the epidermal-dermal junction is composed of four component areas:(1) the basal cell plasma membrane with its specialized attachment devices or hemidesmosomes, (2) an electron-lucent area, the lamina lucida, (3) the basal lamina, and (4) the sub-basal lamina fibrous components, including anchoring fibrils, dermal microfibril bundles, and collagen fibers. The light microscopic \"basement membrane\" comprises only the sub-basal lamina fibrous zone. Other cell types, including melanocytes and Merkel cells, are also found at the epidermal-dermal junction. Structures at the junction derive their origin from the epidermis and dermis: the basal lamina is primarily of epidermal origin, the anchoring fibrils of dermal origin. The junction serves the following functions: (1) epidermal-dermal adherence, (2) mechanical support for the epidermis, and (3) a barrier to the exchange of cells and of some large molecules across the junction.", "contents": "The epidermal-dermal junction. Ultrastructurally, the epidermal-dermal junction is composed of four component areas:(1) the basal cell plasma membrane with its specialized attachment devices or hemidesmosomes, (2) an electron-lucent area, the lamina lucida, (3) the basal lamina, and (4) the sub-basal lamina fibrous components, including anchoring fibrils, dermal microfibril bundles, and collagen fibers. The light microscopic \"basement membrane\" comprises only the sub-basal lamina fibrous zone. Other cell types, including melanocytes and Merkel cells, are also found at the epidermal-dermal junction. Structures at the junction derive their origin from the epidermis and dermis: the basal lamina is primarily of epidermal origin, the anchoring fibrils of dermal origin. The junction serves the following functions: (1) epidermal-dermal adherence, (2) mechanical support for the epidermis, and (3) a barrier to the exchange of cells and of some large molecules across the junction."}
{"id": "PMID:1097543", "title": "Immunoglobulin classes of antibody to hepatitis B core antigen.", "content": "Antibody to hepatitis B core antigen in the sera of 10 patients with acute viral hepatitis B, 20 patients with chronic hepatitis B, and 30 carriers of chronic hepatitis B surface antigen was assayed by the indirect immunofluorescence test. The activity of antibody to hepatitis B core antigen in the sera of these three groups of patients was identified as belonging to immunoglobulin classes G, M, A, and D. Antibody to hepatitis B core antigen was predominantly of IgG and IgM classes in all three groups of patients.", "contents": "Immunoglobulin classes of antibody to hepatitis B core antigen. Antibody to hepatitis B core antigen in the sera of 10 patients with acute viral hepatitis B, 20 patients with chronic hepatitis B, and 30 carriers of chronic hepatitis B surface antigen was assayed by the indirect immunofluorescence test. The activity of antibody to hepatitis B core antigen in the sera of these three groups of patients was identified as belonging to immunoglobulin classes G, M, A, and D. Antibody to hepatitis B core antigen was predominantly of IgG and IgM classes in all three groups of patients."}
{"id": "PMID:1097544", "title": "Spread of Streptococcus pneumoniae in families. I. Carriage rates and distribution of types.", "content": "The mechanisms by which Streptococcus pneumoniae spreads from person to person are poorly understood. In this study, optimal methods for sampling, isolation, and identification of S. pneumoniae from healthy carriers were investigated. Factors influencing carriage rates were analyzed. Findings included the importance of pharyngeal rather than of nasal sampling in adults, the greater sensitivity of mouse inoculation compared with direct streaking of blood agar plates, and the superiority of the Quellung reaction with omniserum over the optochin disk as a means of identification of S. pneumoniae. Carriage rates were highest in children of preschool (35%) or grammar school (29%) age and in adults with preschool children in the family (18%). Rates were lowest in adults without preschool children in the family (2%-9%).", "contents": "Spread of Streptococcus pneumoniae in families. I. Carriage rates and distribution of types. The mechanisms by which Streptococcus pneumoniae spreads from person to person are poorly understood. In this study, optimal methods for sampling, isolation, and identification of S. pneumoniae from healthy carriers were investigated. Factors influencing carriage rates were analyzed. Findings included the importance of pharyngeal rather than of nasal sampling in adults, the greater sensitivity of mouse inoculation compared with direct streaking of blood agar plates, and the superiority of the Quellung reaction with omniserum over the optochin disk as a means of identification of S. pneumoniae. Carriage rates were highest in children of preschool (35%) or grammar school (29%) age and in adults with preschool children in the family (18%). Rates were lowest in adults without preschool children in the family (2%-9%)."}
{"id": "PMID:1097545", "title": "Synergism of amphotericin B and 5-fluorocytosine for candida species.", "content": "The in vitro activity of amphotericin B and 5-fluorocytosine against 40 isolates of different species of Candida was studied. Synergism was seen in 35 of 40 organisms studied (88%) with achievable serum concentrations of the antibiotics. Synergism was striking in many strains that were resistant to 5-fluorocytosine. When the minimal inhibitory concentration of 5-fluorocytosine was greater than 400 mug/ml, synergism was seen in 18 of 20 isolates of Candida (90%).", "contents": "Synergism of amphotericin B and 5-fluorocytosine for candida species. The in vitro activity of amphotericin B and 5-fluorocytosine against 40 isolates of different species of Candida was studied. Synergism was seen in 35 of 40 organisms studied (88%) with achievable serum concentrations of the antibiotics. Synergism was striking in many strains that were resistant to 5-fluorocytosine. When the minimal inhibitory concentration of 5-fluorocytosine was greater than 400 mug/ml, synergism was seen in 18 of 20 isolates of Candida (90%)."}
{"id": "PMID:1097546", "title": "New knowledge of chlamydiae and the diseases they cause.", "content": "The trachoma and LGV organisms, the human pathogens of the species C. trachomatis, cause oculogenital infections and disease syndromes of the eye and genital tract. The incidence of the most prominent disease, endemic trachoma with eye-to-eye transmission, is decreasing all over the world. At the same time there is increasing recognition of high-frequency venereal infections with trachoma organisms and of the genital disease and occasional ocular disease that they cause. Laboratory techniques for diagnosis and investigation are improving, but work with these interesting intermediate agents remains more difficult than that with many other microorganisms. Proper recognition of the diseases is important because specific therapy is available.", "contents": "New knowledge of chlamydiae and the diseases they cause. The trachoma and LGV organisms, the human pathogens of the species C. trachomatis, cause oculogenital infections and disease syndromes of the eye and genital tract. The incidence of the most prominent disease, endemic trachoma with eye-to-eye transmission, is decreasing all over the world. At the same time there is increasing recognition of high-frequency venereal infections with trachoma organisms and of the genital disease and occasional ocular disease that they cause. Laboratory techniques for diagnosis and investigation are improving, but work with these interesting intermediate agents remains more difficult than that with many other microorganisms. Proper recognition of the diseases is important because specific therapy is available."}
{"id": "PMID:1097548", "title": "Differential pulmonary residual volume determinations with radioactive xenon.", "content": "A radioactive xenon inhalation procedure was used to determine differential residual volumes (right lung vs. left lung) in 28 normal subjects (6 of whom smoked approximately 1 package of cigarettes per day).  The average right lung residual volume represented 54% of the total residual volume, whereas the average left lung residual volumerepresented 46% of the total.  In addition, all of the subjects were studied by a standard helium dilution technique and found to have total lung residual comparable to the total lung residual volume as determined by the xenon technique.  There was no statistical difference between the data obtained from cigarette smokers vs. nonsmokers.  The noninvasive nature of the xenon technique makes this a useful procedure in determining differential residual volumes.", "contents": "Differential pulmonary residual volume determinations with radioactive xenon. A radioactive xenon inhalation procedure was used to determine differential residual volumes (right lung vs. left lung) in 28 normal subjects (6 of whom smoked approximately 1 package of cigarettes per day).  The average right lung residual volume represented 54% of the total residual volume, whereas the average left lung residual volumerepresented 46% of the total.  In addition, all of the subjects were studied by a standard helium dilution technique and found to have total lung residual comparable to the total lung residual volume as determined by the xenon technique.  There was no statistical difference between the data obtained from cigarette smokers vs. nonsmokers.  The noninvasive nature of the xenon technique makes this a useful procedure in determining differential residual volumes."}
{"id": "PMID:1097549", "title": "Incidence and titers of antinuclear, antismooth muscle, and other autoantibodies in blood donors.", "content": "Sera from 107 blood donors ranging in age from 18 to 66 years were tested by indirect immunofluorescence for titers of various autoantibodies. These were matched for sex in diffferent age brackets. Antinuclear antibody (ANA) titiers of 10 or more appeared in 45 per cent of the sera examined. However, only 5.6 per cent had ANA titers of 80 or more, and only one subject had a titer of 160. ANA titers of 80 or greater were only found in the oldest age group (51 years or older). In those subjects with detectable ANA, 57.7 per cent were female and 32.8 per cent were male. The prevalence of thyroid cytoplasm and gastric parietal cell antibodies also appeared both age and sex related, while smooth muscle antibodies did not. Anti-DNA antibody, pemphigus antibody, bullous pemphigoid antibody, and mitochondrial antibody were detected in none of the sera tested. No relationships were seen in pairs of autoantibodies.", "contents": "Incidence and titers of antinuclear, antismooth muscle, and other autoantibodies in blood donors. Sera from 107 blood donors ranging in age from 18 to 66 years were tested by indirect immunofluorescence for titers of various autoantibodies. These were matched for sex in diffferent age brackets. Antinuclear antibody (ANA) titiers of 10 or more appeared in 45 per cent of the sera examined. However, only 5.6 per cent had ANA titers of 80 or more, and only one subject had a titer of 160. ANA titers of 80 or greater were only found in the oldest age group (51 years or older). In those subjects with detectable ANA, 57.7 per cent were female and 32.8 per cent were male. The prevalence of thyroid cytoplasm and gastric parietal cell antibodies also appeared both age and sex related, while smooth muscle antibodies did not. Anti-DNA antibody, pemphigus antibody, bullous pemphigoid antibody, and mitochondrial antibody were detected in none of the sera tested. No relationships were seen in pairs of autoantibodies."}
{"id": "PMID:1097550", "title": "Chemotaxis as a preparative technique for human polymorphonuclear leukocytes.", "content": "Human peripheral blood phagocytic cells were prepared in large quantities utilizing their chemotactic characteristics. Cells were isolated using an enlarged version of the Boyden chemotactic chamber and were collected after migrating through a Millipore membrane into a solution containing chemotactic factor. Chemotactic factors consisted of normal human serum, casein-saturated medium, and a combination of human serum and casein-saturated medium. The most effective attractant was the mixture of casein and fresh human serum. Yields of up to 24.8 times 10(6) cells could be attained from a starting volume of approximately 12 c.c. of peripheral blood. These cells were greater than 95 per cent polymorphonuclear leukocytes with the remaining cells being monocytes. No erythrocytes or lymphocytes contaminated these preparations. The advantages of this technique are as follows: (1) large quantities of human peripheral blood phagocytes consisting of greater than 95 per cent polymorphonuclear cells can be obtained: (2) these cells are known to be biologically active sice chemotaxis is a requirement for their isolation; (3) the separative method is dependent on chemotactic properties rather than sedimentation characteristics; and finally (4) the resulting cell preparations are virtually devoid of lymphocytes and erythrocytes.", "contents": "Chemotaxis as a preparative technique for human polymorphonuclear leukocytes. Human peripheral blood phagocytic cells were prepared in large quantities utilizing their chemotactic characteristics. Cells were isolated using an enlarged version of the Boyden chemotactic chamber and were collected after migrating through a Millipore membrane into a solution containing chemotactic factor. Chemotactic factors consisted of normal human serum, casein-saturated medium, and a combination of human serum and casein-saturated medium. The most effective attractant was the mixture of casein and fresh human serum. Yields of up to 24.8 times 10(6) cells could be attained from a starting volume of approximately 12 c.c. of peripheral blood. These cells were greater than 95 per cent polymorphonuclear leukocytes with the remaining cells being monocytes. No erythrocytes or lymphocytes contaminated these preparations. The advantages of this technique are as follows: (1) large quantities of human peripheral blood phagocytes consisting of greater than 95 per cent polymorphonuclear cells can be obtained: (2) these cells are known to be biologically active sice chemotaxis is a requirement for their isolation; (3) the separative method is dependent on chemotactic properties rather than sedimentation characteristics; and finally (4) the resulting cell preparations are virtually devoid of lymphocytes and erythrocytes."}
{"id": "PMID:1097551", "title": "Radioimmunoassay, acetylating radio-enzymatic assay, and microbioassay of gentamicin: a comparative study.", "content": "Gentamicin is an aminoglycoside antibiotic widely used to treat gram-negative bacillary infections. Because it has a low therapeutic index, monitoring of serum levels may help to insure adequacy of dosage and avoid toxicity. Microbiological assays are relatively slow and can be complicated by the presence of other antimicrobials. Radioimmunoassay (RIA) and acetylating radio-enzymatic assay (ARA) are new methods for gentamicin assay which offer the following advantages: rapidity (less than 3 hours); no interference by other antibiotics; RIA is extremely sensitive and ARA is versatile (being useful in the measurement of other aminoglycosides). Correlation coefficients determined by linear regression analysis of assays on 36 patient samples performed in duplicate on 2 different days demonstrated no significant difference in measurement of gentamicin by each of the methods. Factors such as numbers of specimens, cost, and time involved will affect the decision of the method to be applied in individual laboratories.", "contents": "Radioimmunoassay, acetylating radio-enzymatic assay, and microbioassay of gentamicin: a comparative study. Gentamicin is an aminoglycoside antibiotic widely used to treat gram-negative bacillary infections. Because it has a low therapeutic index, monitoring of serum levels may help to insure adequacy of dosage and avoid toxicity. Microbiological assays are relatively slow and can be complicated by the presence of other antimicrobials. Radioimmunoassay (RIA) and acetylating radio-enzymatic assay (ARA) are new methods for gentamicin assay which offer the following advantages: rapidity (less than 3 hours); no interference by other antibiotics; RIA is extremely sensitive and ARA is versatile (being useful in the measurement of other aminoglycosides). Correlation coefficients determined by linear regression analysis of assays on 36 patient samples performed in duplicate on 2 different days demonstrated no significant difference in measurement of gentamicin by each of the methods. Factors such as numbers of specimens, cost, and time involved will affect the decision of the method to be applied in individual laboratories."}
{"id": "PMID:1097552", "title": "Histopathological findings in cochlear implants in cats.", "content": "This histopathological study on cats has shown that electrodes may be implanted into the cochlea through the round window or an opening drilled over the middle and apical turns without loss of the spiral ganglion cells or auditory nerve fibres. It has also shown that infection, leading to a labyrinthitis, was more likely to occur when an opening was drilled in the cochlea, and this could lead to a marked destruction of the cochlear structures, and loss of the spiral ganglion cells.", "contents": "Histopathological findings in cochlear implants in cats. This histopathological study on cats has shown that electrodes may be implanted into the cochlea through the round window or an opening drilled over the middle and apical turns without loss of the spiral ganglion cells or auditory nerve fibres. It has also shown that infection, leading to a labyrinthitis, was more likely to occur when an opening was drilled in the cochlea, and this could lead to a marked destruction of the cochlear structures, and loss of the spiral ganglion cells."}
{"id": "PMID:1097553", "title": "Leprosy of the upper respiratory tract. A clinical bacteriological, histopathological and histochemical study of twenty cases.", "content": "Twenty cases clinically diagnosed as leprosy were thoroughly examined for E.N.T. lesions. These lesions were subjected to bacteriological, histopathological and histochemical studies. The results have been tabulated and discussed with special stress on some findings which are of help in diagnosing the disease.", "contents": "Leprosy of the upper respiratory tract. A clinical bacteriological, histopathological and histochemical study of twenty cases. Twenty cases clinically diagnosed as leprosy were thoroughly examined for E.N.T. lesions. These lesions were subjected to bacteriological, histopathological and histochemical studies. The results have been tabulated and discussed with special stress on some findings which are of help in diagnosing the disease."}
{"id": "PMID:1097558", "title": "P wave abnormalities in the orthogonal electrocardiogram: Correlation with ventricular overload in pulmonic and aortic valvular heart disease.", "content": "The correlation between several P wave measurements form the orthogonal electrocardiogram (SVEC III lead system) extracted by computer analysis and simple hemodynamic parameters related to ventricular dysfunction was studied in two groups of patients. Group I consisted of 32 patients with pulmonic valvular stenosis and intact interventricular septum. There was a significant correlation between electrocardiographic criteria of right atrial overload and the two hemodynamic parameters studied: peak pulmonic systolic pressure gradient and right ventricular end diastolic pressure (r = 0.502, p smaller than 0.005 and r = 0.661, p smaller than 0.001 respectively). Group II consisted of 49 patients with aortic valve disease. In this group, a significant correlation between the electrocardiographic parameters of left atrial overload and the left ventricular end diastolic pressure could be demonstrated only be a multivariate regression analysis (r = 0.630, p smaller than 0.005). The P wave measurements that are well correlated with the ventricular end diastolic pressure can be considered as valuable criteria for atrial enlargement secondary to a decrease of ventricular compliance, such as seen in ventricular hypertrophy, failure or in ventricular constrictive or restrictive diseases. The pathophysiologic mechanisms of the influence of the ventricular overload (dysfunction) on the atrial function and the resulting P wave changes are discussed.", "contents": "P wave abnormalities in the orthogonal electrocardiogram: Correlation with ventricular overload in pulmonic and aortic valvular heart disease. The correlation between several P wave measurements form the orthogonal electrocardiogram (SVEC III lead system) extracted by computer analysis and simple hemodynamic parameters related to ventricular dysfunction was studied in two groups of patients. Group I consisted of 32 patients with pulmonic valvular stenosis and intact interventricular septum. There was a significant correlation between electrocardiographic criteria of right atrial overload and the two hemodynamic parameters studied: peak pulmonic systolic pressure gradient and right ventricular end diastolic pressure (r = 0.502, p smaller than 0.005 and r = 0.661, p smaller than 0.001 respectively). Group II consisted of 49 patients with aortic valve disease. In this group, a significant correlation between the electrocardiographic parameters of left atrial overload and the left ventricular end diastolic pressure could be demonstrated only be a multivariate regression analysis (r = 0.630, p smaller than 0.005). The P wave measurements that are well correlated with the ventricular end diastolic pressure can be considered as valuable criteria for atrial enlargement secondary to a decrease of ventricular compliance, such as seen in ventricular hypertrophy, failure or in ventricular constrictive or restrictive diseases. The pathophysiologic mechanisms of the influence of the ventricular overload (dysfunction) on the atrial function and the resulting P wave changes are discussed."}
{"id": "PMID:1097559", "title": "Semi-automated ECG processing: A simple method to improve efficiency in ECG laboratories.", "content": "A method to expedite processing of electrocardiograms (ECGs) is described. The hardware configuration utilizes conventional equipment, and the ECG data is stored in magnetic data cards. The electrocardiographer's interpretation is made using a specially developed code of 253 diagnostic statements of 2 to 9 words each. A minicomputer converts the code into full alpha-numeric description and 2 characters into English statements. The diagnostic print-out appears in the same page as the reproduction of the original ECG data. This system has significantly reduced the ECG processing time, freed manpower to increase availability of technicians and decreased the size of permanent files.", "contents": "Semi-automated ECG processing: A simple method to improve efficiency in ECG laboratories. A method to expedite processing of electrocardiograms (ECGs) is described. The hardware configuration utilizes conventional equipment, and the ECG data is stored in magnetic data cards. The electrocardiographer's interpretation is made using a specially developed code of 253 diagnostic statements of 2 to 9 words each. A minicomputer converts the code into full alpha-numeric description and 2 characters into English statements. The diagnostic print-out appears in the same page as the reproduction of the original ECG data. This system has significantly reduced the ECG processing time, freed manpower to increase availability of technicians and decreased the size of permanent files."}
{"id": "PMID:1097560", "title": "Quantitative vectorcardiographic criteria for the differentiation between atrial septal defects of primum and secundum types.", "content": "From a population of 304 patients who were operated on for atrial septal defects, 25 patients with primum defects and 78 patients with secundum defects were studied with regard to the ability of various quantitative axial lead vectorcardiographic (VCG) data to predict the anatomical type of defect. The patients included all in whom there had been preoperative doubt concerning the classification. By applying the electrocardiographic (ECG) mean frontal QRS axis, 8 patients were erroneously classified, and 14 could not be classified because no definite QRS resultant was present in the frontal plane (diagnostic performance index 0.85). Several VCG criteria showed a better performance, the best being sums of 10 msec Y lead amplitudes from 20 to 50 msec after QRS onset. These criteria, which reflect the superior shift of the middle part of the QRS complex in primum defects, reduced the number of misclassifications to a minimum of 4 (performance 0.96). A combination of this criterion with one based on the duration of the initial inferior QRS vectors gave some further improvement. This simple combination was not surpassed by classification performed by a stepwise discriminant analysis computer program (BMD 07M).", "contents": "Quantitative vectorcardiographic criteria for the differentiation between atrial septal defects of primum and secundum types. From a population of 304 patients who were operated on for atrial septal defects, 25 patients with primum defects and 78 patients with secundum defects were studied with regard to the ability of various quantitative axial lead vectorcardiographic (VCG) data to predict the anatomical type of defect. The patients included all in whom there had been preoperative doubt concerning the classification. By applying the electrocardiographic (ECG) mean frontal QRS axis, 8 patients were erroneously classified, and 14 could not be classified because no definite QRS resultant was present in the frontal plane (diagnostic performance index 0.85). Several VCG criteria showed a better performance, the best being sums of 10 msec Y lead amplitudes from 20 to 50 msec after QRS onset. These criteria, which reflect the superior shift of the middle part of the QRS complex in primum defects, reduced the number of misclassifications to a minimum of 4 (performance 0.96). A combination of this criterion with one based on the duration of the initial inferior QRS vectors gave some further improvement. This simple combination was not surpassed by classification performed by a stepwise discriminant analysis computer program (BMD 07M)."}
{"id": "PMID:1097561", "title": "Electrocardiographic screening by special purpose computer. Correlation with twelve-lead electrocardiogram.", "content": "An electrocardiometer (ECM) which computes the T/R ratio of Lead I and simultaneously prints out Lead I was used to screen 1,004 consecutive hospitalized patients for EKG abnormalities. When the combined meter and Lead I print out analyses were used, 77.9% of abnormal EKG's were identified with a false-positive rate of less than 4%. The method is highly specific for detecting EKG abnormalities associated with anterior and lateral left ventricular disease. It could be used as an inexpensive mass screening device for electrocardiographic risk factors associated with coronary disease.", "contents": "Electrocardiographic screening by special purpose computer. Correlation with twelve-lead electrocardiogram. An electrocardiometer (ECM) which computes the T/R ratio of Lead I and simultaneously prints out Lead I was used to screen 1,004 consecutive hospitalized patients for EKG abnormalities. When the combined meter and Lead I print out analyses were used, 77.9% of abnormal EKG's were identified with a false-positive rate of less than 4%. The method is highly specific for detecting EKG abnormalities associated with anterior and lateral left ventricular disease. It could be used as an inexpensive mass screening device for electrocardiographic risk factors associated with coronary disease."}
{"id": "PMID:1097563", "title": "The responsiveness of the anterior pituitary gland to luteinizing hormone releasing factor in rats exposed to constant light.", "content": "The responsiveness of the anterior pituitary gland to synthetic luteinizing hormone releasing factor (LH-RF) was tested in rats exposed to constant light. At a dosage of 50 ng LH-RF/100 g body wt the mean maximal increments in plasma LH and FSH were similar to those at 10.00 h of pro-oestrus. The increments in the plasma gonadotrophins at dosages of 500 and 1000 ng LH-RF/100 g body wt did not differ significantly from those at 250 ng LH-RF/100 g body wt. These findings suggest that, in contrast to rats which exhibit regular oestrous cycles, the preovulatory (post-coital) release of LH in rats exposed to constant light may depend almost entirely on the release of a relatively large amount of LH-RF into hypophysial portal vessel blood. Whereas in pro-oestrous animals a relatively small fraction of the readily releasable pool of LH is released during the spontaneous preovulatory surge, in rats exposed to constant light most releasable LH appears to be discharged during the reflex preovulatory surge of this hormone. The concentrations of radioimmunoassayable FSH in blood samples withdrawn before the injection of LH-RF support the view that FSH secretion in the rat is increased by constant exposure to light.", "contents": "The responsiveness of the anterior pituitary gland to luteinizing hormone releasing factor in rats exposed to constant light. The responsiveness of the anterior pituitary gland to synthetic luteinizing hormone releasing factor (LH-RF) was tested in rats exposed to constant light. At a dosage of 50 ng LH-RF/100 g body wt the mean maximal increments in plasma LH and FSH were similar to those at 10.00 h of pro-oestrus. The increments in the plasma gonadotrophins at dosages of 500 and 1000 ng LH-RF/100 g body wt did not differ significantly from those at 250 ng LH-RF/100 g body wt. These findings suggest that, in contrast to rats which exhibit regular oestrous cycles, the preovulatory (post-coital) release of LH in rats exposed to constant light may depend almost entirely on the release of a relatively large amount of LH-RF into hypophysial portal vessel blood. Whereas in pro-oestrous animals a relatively small fraction of the readily releasable pool of LH is released during the spontaneous preovulatory surge, in rats exposed to constant light most releasable LH appears to be discharged during the reflex preovulatory surge of this hormone. The concentrations of radioimmunoassayable FSH in blood samples withdrawn before the injection of LH-RF support the view that FSH secretion in the rat is increased by constant exposure to light."}
{"id": "PMID:1097569", "title": "The effects of minute direct electrical currents on cultured chick embryo trigeminal ganglia.", "content": "The effects of low levels of electric current were determined on organ cultures of 6- to 19-day-old chick embryo trigeminal ganglia. Current levels ranged from 0.00115 to 11.5 nA/mm2; the time of electrical treatment varied from 2 h to 96 h. Low levels of electricity were found to have at least three major effects on this system of mixed cellular types: (1) Outgrowth of nerve fibers from the explant was enriched. This resulted in a greater number of fibers which were longer and more highly branched than those in control cultures. (2) Survival of neurons within the original explanted ganglion was enchanced by treatment with electricity. The presence of healthy neurons was found in 93.1% of the treated cultures and in 53.5% of untreated cultures. (3) Neurons, fibers and non-neural cells were stimulated to grow in the direction of the cathode. The rate of cathodal migration was calculated to be 0.1 mm/h (2.4 mm/day) in an explant from 12-day-old embryo. No differences were observed between surviving treated and control cultures in histochemical tests for acetycholinesterase. A similarity between the action of low levels of electrical current and nerve growth factor (NGF) is suggested.", "contents": "The effects of minute direct electrical currents on cultured chick embryo trigeminal ganglia. The effects of low levels of electric current were determined on organ cultures of 6- to 19-day-old chick embryo trigeminal ganglia. Current levels ranged from 0.00115 to 11.5 nA/mm2; the time of electrical treatment varied from 2 h to 96 h. Low levels of electricity were found to have at least three major effects on this system of mixed cellular types: (1) Outgrowth of nerve fibers from the explant was enriched. This resulted in a greater number of fibers which were longer and more highly branched than those in control cultures. (2) Survival of neurons within the original explanted ganglion was enchanced by treatment with electricity. The presence of healthy neurons was found in 93.1% of the treated cultures and in 53.5% of untreated cultures. (3) Neurons, fibers and non-neural cells were stimulated to grow in the direction of the cathode. The rate of cathodal migration was calculated to be 0.1 mm/h (2.4 mm/day) in an explant from 12-day-old embryo. No differences were observed between surviving treated and control cultures in histochemical tests for acetycholinesterase. A similarity between the action of low levels of electrical current and nerve growth factor (NGF) is suggested."}
{"id": "PMID:1097570", "title": "Histocompatibility studies in a closely bred colony of dogs. V. Mechanisms of cellular adaptation in long-term DL-A identical radiation chimeras.", "content": "20 Cooperstown beagles of known DL-A genotypes (B\" dogs) were exposed to supralethal total body irradiation and received a bone marrow allograft from a DL-A identical donor (A\" dog); the resulting chimeras have survived uneventfully for 882, 1466 days, with no evidence of secondary disease, and have been tolerant to kidney and skin allografts obtained from the donor of marrow. Treatment of \"A\" dogs with serum obtained from their long-term \"B\" chimeras had no significant effect upon the ability of the recipients to reject \"B\" skin allografts...", "contents": "Histocompatibility studies in a closely bred colony of dogs. V. Mechanisms of cellular adaptation in long-term DL-A identical radiation chimeras. 20 Cooperstown beagles of known DL-A genotypes (B\" dogs) were exposed to supralethal total body irradiation and received a bone marrow allograft from a DL-A identical donor (A\" dog); the resulting chimeras have survived uneventfully for 882, 1466 days, with no evidence of secondary disease, and have been tolerant to kidney and skin allografts obtained from the donor of marrow. Treatment of \"A\" dogs with serum obtained from their long-term \"B\" chimeras had no significant effect upon the ability of the recipients to reject \"B\" skin allografts..."}
{"id": "PMID:1097571", "title": "C1q deviation test for the detection of immune complexes, aggregates of IgG, and bacterial products in human serum.", "content": "This report describes a new, rapid, sensitive, and quantitative method for the detection of immune complexes, endotoxins, and other complement activating materials in patients sera utilizing the ability of these substances to react with isolated C1q. The procedure is based on the inhibition of radiolabeled C1q binding to sensitized sheep erythrocytes by C1q-reactive substances in pathological sera. The C1q deviation test may be performed on 50 mu1 of serum, using 1 mug of radiolabeled C1q per sample. The procedure may be completed in 1.5-2 h, it is capable of detecting 5 mug of aggregated human IgG per ml of serum, and its coefficient of variation is 4.2%. Application of the test to the study of 193 sera from 43 patients with Dengue hemorrhagic fever showed a positive correlation between degree of C1q deviation and severity of disease.", "contents": "C1q deviation test for the detection of immune complexes, aggregates of IgG, and bacterial products in human serum. This report describes a new, rapid, sensitive, and quantitative method for the detection of immune complexes, endotoxins, and other complement activating materials in patients sera utilizing the ability of these substances to react with isolated C1q. The procedure is based on the inhibition of radiolabeled C1q binding to sensitized sheep erythrocytes by C1q-reactive substances in pathological sera. The C1q deviation test may be performed on 50 mu1 of serum, using 1 mug of radiolabeled C1q per sample. The procedure may be completed in 1.5-2 h, it is capable of detecting 5 mug of aggregated human IgG per ml of serum, and its coefficient of variation is 4.2%. Application of the test to the study of 193 sera from 43 patients with Dengue hemorrhagic fever showed a positive correlation between degree of C1q deviation and severity of disease."}
{"id": "PMID:1097572", "title": "The modulation of lymphocyte functions by molecules secreted by macrophages. I. Description and partial biochemical analysis.", "content": "Culture fluids of peritoneal exudate cells rich in macrophages stimulated DNA synthesis of thymocytes and, to lesser extent, of spleen cells. We also investigated the effects of culture fluids from macrophages on the in vitro response to a hapten-carrier protein (fluorescein-menocyanin) using spleen cells from immune mice. Macrophage culture fluids contained an activity that increased the plaque-forming cell response of both IgG and IgM class. This increase was observed in the absence of any added hapten protein to the culture. The helper function of T lymphocytes (as evidenced by challenging with the hapten on the homologous carrier) was also increased by the macrophage culture fluid. However, this enhancement was best observed in conditions of relatively low T-cell activity. Also, the macrophage fluid allowed spleen cells of nude athymic mice to make a plaque-forming cell response to sheep red blood cells of both the IgM and IgG class. The macrophage was the cell source of the stimulatory molecule since it was generated only in cultures of macrophages devoid of significant number of lymphocytes. Stimulatory activity was not found in cultures of lymphocytes, mouse embryo cells, or 3T3 cells. The thymocyte stimulatory molecule did not contain H-2 antigens, was resistant to diisopropylfluorophosphate treatment, eluted from Sephadex with a size ranging from 15,000 to 21,000 daltons, and was sensitive to chymotrypsin and pepsin.", "contents": "The modulation of lymphocyte functions by molecules secreted by macrophages. I. Description and partial biochemical analysis. Culture fluids of peritoneal exudate cells rich in macrophages stimulated DNA synthesis of thymocytes and, to lesser extent, of spleen cells. We also investigated the effects of culture fluids from macrophages on the in vitro response to a hapten-carrier protein (fluorescein-menocyanin) using spleen cells from immune mice. Macrophage culture fluids contained an activity that increased the plaque-forming cell response of both IgG and IgM class. This increase was observed in the absence of any added hapten protein to the culture. The helper function of T lymphocytes (as evidenced by challenging with the hapten on the homologous carrier) was also increased by the macrophage culture fluid. However, this enhancement was best observed in conditions of relatively low T-cell activity. Also, the macrophage fluid allowed spleen cells of nude athymic mice to make a plaque-forming cell response to sheep red blood cells of both the IgM and IgG class. The macrophage was the cell source of the stimulatory molecule since it was generated only in cultures of macrophages devoid of significant number of lymphocytes. Stimulatory activity was not found in cultures of lymphocytes, mouse embryo cells, or 3T3 cells. The thymocyte stimulatory molecule did not contain H-2 antigens, was resistant to diisopropylfluorophosphate treatment, eluted from Sephadex with a size ranging from 15,000 to 21,000 daltons, and was sensitive to chymotrypsin and pepsin."}
{"id": "PMID:1097573", "title": "Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens.", "content": "The data presented in this paper establish the finding that multiple specific protective antibodies exist in rabbits in response to immunization with Group B streptococci. The summary in Table I indicates the serological types into which Group B streptococci have been divided on the basis of their antigenic composition. This classification is dependent upon passive protection of mice with antibodies directed against the specific antigens, and types are defined in these terms. Heretofore, it was thought that type-specific polysaccharides accounted for all such protection in Group B streptococci. Certain exceptions of cross-protection between types due to minor polysaccharide determinants soon appeared; cross-protection reactions based on protein determinants in at least two types were also discovered. The present experiments show that specific antibodies directed to either polysaccharide or protein antigens of a single strain can be protective against infection with streptococci containing these antigens.", "contents": "Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens. The data presented in this paper establish the finding that multiple specific protective antibodies exist in rabbits in response to immunization with Group B streptococci. The summary in Table I indicates the serological types into which Group B streptococci have been divided on the basis of their antigenic composition. This classification is dependent upon passive protection of mice with antibodies directed against the specific antigens, and types are defined in these terms. Heretofore, it was thought that type-specific polysaccharides accounted for all such protection in Group B streptococci. Certain exceptions of cross-protection between types due to minor polysaccharide determinants soon appeared; cross-protection reactions based on protein determinants in at least two types were also discovered. The present experiments show that specific antibodies directed to either polysaccharide or protein antigens of a single strain can be protective against infection with streptococci containing these antigens."}
{"id": "PMID:1097574", "title": "Histoincompatibility and maternal immunological status as determinants of fetoplacental weight and litter size in rodents.", "content": "Studies conducted upon inbred strains of mice, hamsters and rats have shown that following the interstrain matings the now familiar covert reactivity of pregnant females to the alloantigens of their conceptuses may benefit the latter in two ways; firstly, it exerts a significant influence upon placental weight, and indirectly upon the birth weight of the fetus-allogeneic placentas tending to be heavier than syngeneic placentas, and mothers specifically presensitized against alien paternal tissue antigens gestate fetuses with heavier placentas than normal females. Specifically tolerant mothers, on the other hand, produce smaller, F1 hybrid, fetoplacental (fp) units. The classic notion that the disparity between the birth weights of F1 hybrid and homozygous offspring is due to hybrid vigor has been challenged by the finding that DA and (DA times F1)F1 hybrid blastocysts transferred to the uteri of genetically tolerant (DA times F1)F1 hybrid rats produce fp units of similar weight Maternal immunological reactivity against the fetus qua allograft may make a significant contribution here. Additional support for the premise that maternal reactivity against fetal alloantigens in some way promotes the growth of the fp unit was afforded by the finding that excision of the para-aortic lymph nodes (which drain the uterine horns) from females before interstrain matings resulted in smaller fp units than in females subjected to sham operations. The finding with one rat strain combination that passive immunization of females with serum against their F1 hybrid conceptuses promoted the growth of the latter suggests that a humoral rather than a cellular immunity may be involved. Secondly, in the three species studied, it was observed that genetic disparity between a conceptus and its mother significantly improved its chances of implantation and development to term.", "contents": "Histoincompatibility and maternal immunological status as determinants of fetoplacental weight and litter size in rodents. Studies conducted upon inbred strains of mice, hamsters and rats have shown that following the interstrain matings the now familiar covert reactivity of pregnant females to the alloantigens of their conceptuses may benefit the latter in two ways; firstly, it exerts a significant influence upon placental weight, and indirectly upon the birth weight of the fetus-allogeneic placentas tending to be heavier than syngeneic placentas, and mothers specifically presensitized against alien paternal tissue antigens gestate fetuses with heavier placentas than normal females. Specifically tolerant mothers, on the other hand, produce smaller, F1 hybrid, fetoplacental (fp) units. The classic notion that the disparity between the birth weights of F1 hybrid and homozygous offspring is due to hybrid vigor has been challenged by the finding that DA and (DA times F1)F1 hybrid blastocysts transferred to the uteri of genetically tolerant (DA times F1)F1 hybrid rats produce fp units of similar weight Maternal immunological reactivity against the fetus qua allograft may make a significant contribution here. Additional support for the premise that maternal reactivity against fetal alloantigens in some way promotes the growth of the fp unit was afforded by the finding that excision of the para-aortic lymph nodes (which drain the uterine horns) from females before interstrain matings resulted in smaller fp units than in females subjected to sham operations. The finding with one rat strain combination that passive immunization of females with serum against their F1 hybrid conceptuses promoted the growth of the latter suggests that a humoral rather than a cellular immunity may be involved. Secondly, in the three species studied, it was observed that genetic disparity between a conceptus and its mother significantly improved its chances of implantation and development to term."}
{"id": "PMID:1097575", "title": "Genetical control of B-cell responses. IV. Inheritance of the unresponsiveness to lipopolysaccharides.", "content": "The inheritance of B-cell responsiveness to lipopolysaccharide (LPS) was studied in 55 crosses between mice of the low-responder strain C3H/HeJ and the high-responder strains B10.5M and C3H/Tif. F1 hybrid mice between the low-and the high-responder strains, showed in every case responses which were intermediate between the responses obtained with each parent. The responsiveness among F2 hybrid and backcross mice to either high- or low-responder parents, segregated into intermediate, high, or low categories, respectively. The present results are compatible with the hypothesis that responsiveness to LPS is determined by one single, codominantly expressed, autosomal gene. The capacity to develop a specific thymus-independent response to a hapten-LPS conjugate, also under genetical control, was found to segregate together with the capacity to develop polyclonal responses to LPS.", "contents": "Genetical control of B-cell responses. IV. Inheritance of the unresponsiveness to lipopolysaccharides. The inheritance of B-cell responsiveness to lipopolysaccharide (LPS) was studied in 55 crosses between mice of the low-responder strain C3H/HeJ and the high-responder strains B10.5M and C3H/Tif. F1 hybrid mice between the low-and the high-responder strains, showed in every case responses which were intermediate between the responses obtained with each parent. The responsiveness among F2 hybrid and backcross mice to either high- or low-responder parents, segregated into intermediate, high, or low categories, respectively. The present results are compatible with the hypothesis that responsiveness to LPS is determined by one single, codominantly expressed, autosomal gene. The capacity to develop a specific thymus-independent response to a hapten-LPS conjugate, also under genetical control, was found to segregate together with the capacity to develop polyclonal responses to LPS."}
{"id": "PMID:1097576", "title": "Distribution of fibroblast surface antigen in the developing chick embryo.", "content": "Fibroblast surface (SE) antigen is present in fibrillar surface structures of cultured normal fibroblasts, shed to the extracellular medium, and is also found in circulation (serum and plasma). Malignant fibroblasts (transformed by viruses) do not express SF antigen on the cell surface. In this study the in vivo differentiation and distribution of SF antigen has been investigated in the developing chick embryo using cryostat sections and immunofluorescence. The major findings were: (a) SF antigen was detectable in the loose connective tissue of very early (2-to 3-day old) embryos. (b) Condensation of SF antigen was seen in various boundary membranes such as the glomerular and tubular basement membranes of the kidney, the boundary membranes of the notochord, yolk sac, and vitelline membranes and liver sinusoids. (c) SF antigen was found to be cell-type specific. It was seen as a fibrillar network in the loose connective tissue of different organs but not in the parenchymal cells. It was not found in muscle cells at any stage of development. (d) The antigen was present in the undifferentiated mesenchymal cells of the kidney; but not found after their development into epithelial cells of the secretory tubules. (e) Both in vivo and in fibroblast cultures SF antigen was distributed as a fibrillar network. These data indicate that SF antigen is a \"differentiation antigen\" restricted to certain cells of mesenchymal origin and character, and that is accumulates in the connective tissue during embryogenesis.", "contents": "Distribution of fibroblast surface antigen in the developing chick embryo. Fibroblast surface (SE) antigen is present in fibrillar surface structures of cultured normal fibroblasts, shed to the extracellular medium, and is also found in circulation (serum and plasma). Malignant fibroblasts (transformed by viruses) do not express SF antigen on the cell surface. In this study the in vivo differentiation and distribution of SF antigen has been investigated in the developing chick embryo using cryostat sections and immunofluorescence. The major findings were: (a) SF antigen was detectable in the loose connective tissue of very early (2-to 3-day old) embryos. (b) Condensation of SF antigen was seen in various boundary membranes such as the glomerular and tubular basement membranes of the kidney, the boundary membranes of the notochord, yolk sac, and vitelline membranes and liver sinusoids. (c) SF antigen was found to be cell-type specific. It was seen as a fibrillar network in the loose connective tissue of different organs but not in the parenchymal cells. It was not found in muscle cells at any stage of development. (d) The antigen was present in the undifferentiated mesenchymal cells of the kidney; but not found after their development into epithelial cells of the secretory tubules. (e) Both in vivo and in fibroblast cultures SF antigen was distributed as a fibrillar network. These data indicate that SF antigen is a \"differentiation antigen\" restricted to certain cells of mesenchymal origin and character, and that is accumulates in the connective tissue during embryogenesis."}
{"id": "PMID:1097577", "title": "In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells.", "content": "Specific selection of antigen-responsive guinea pig peritoneal exudate lymphocytes (PELs) was achieved by a selection culture procedure. This procedure involved the addition of PELs from immune donors to monolayers of antigen-pulsed adherent peritoneal exudate cells from nonprimed syngeneic donors. PELs which failed to adhere were discarded at 24 and 48 h; after 1 wk of culture, lymphocytes were obtained which were highly responsive to the antigen for which they were selected but which demonstrated little or no response to other antigens to which the original donor of the lymphocyte was immune. These selected cells were largely T lymphocytes and could be maintained in culture for 2-5 wk in an antigen-responsive state and, in 20-30% of cases, for 8-10 wk in an antigen-independent state.", "contents": "In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells. Specific selection of antigen-responsive guinea pig peritoneal exudate lymphocytes (PELs) was achieved by a selection culture procedure. This procedure involved the addition of PELs from immune donors to monolayers of antigen-pulsed adherent peritoneal exudate cells from nonprimed syngeneic donors. PELs which failed to adhere were discarded at 24 and 48 h; after 1 wk of culture, lymphocytes were obtained which were highly responsive to the antigen for which they were selected but which demonstrated little or no response to other antigens to which the original donor of the lymphocyte was immune. These selected cells were largely T lymphocytes and could be maintained in culture for 2-5 wk in an antigen-responsive state and, in 20-30% of cases, for 8-10 wk in an antigen-independent state."}
{"id": "PMID:1097578", "title": "Selective utilization of clinical diagnosis in treatment of pharyngitis.", "content": "Exudate, adenopathy, and fever were used to predict clinically whether pharyngitis was streptococcal, nonstreptococcal, or questionable in 466 adults and 234 children. Clinical accuracy was: nonstreptococcal - adults 94.6 percent, children 86.9 percent; streptococcal - adults 44.2 percent, children 53.5 percent. Significance of results was determined by calculating the cost of routine cultures compared to risk of rheumatic fever. The economic justification of cultures was lowest in nonstreptococcal adults, streptococcal adults, and streptococcal children. Therapy of streptococcal patients presented an additional risk: anaphylaxis. The fatality risk after penicillin injection compared to the increased risk of rheumatic fever after oral penicillin is: adults 7.5 percent (clinical diagnosis), 3.3 percent (laboratory diagnosis); children 1.8 percent and 0.8 percent respectively. It is recommended that throat culture be obtained for all questionable patients and clinically nonstreptococcal children. Penicillin should be administered orally in the majority of adults.", "contents": "Selective utilization of clinical diagnosis in treatment of pharyngitis. Exudate, adenopathy, and fever were used to predict clinically whether pharyngitis was streptococcal, nonstreptococcal, or questionable in 466 adults and 234 children. Clinical accuracy was: nonstreptococcal - adults 94.6 percent, children 86.9 percent; streptococcal - adults 44.2 percent, children 53.5 percent. Significance of results was determined by calculating the cost of routine cultures compared to risk of rheumatic fever. The economic justification of cultures was lowest in nonstreptococcal adults, streptococcal adults, and streptococcal children. Therapy of streptococcal patients presented an additional risk: anaphylaxis. The fatality risk after penicillin injection compared to the increased risk of rheumatic fever after oral penicillin is: adults 7.5 percent (clinical diagnosis), 3.3 percent (laboratory diagnosis); children 1.8 percent and 0.8 percent respectively. It is recommended that throat culture be obtained for all questionable patients and clinically nonstreptococcal children. Penicillin should be administered orally in the majority of adults."}
{"id": "PMID:1097579", "title": "Hematospermia.", "content": "During a 15-year period, 200 patients with hematospermia were seen at the Mayo Clinic. General physical examination, including digital rectal palpation and urinalysis, was performed on all 200 patients. Of these, 26 had no additional urologic evaluation. In the remaining 174 patients, cystoscopy and K.U.B. roentgenography were done. The results of urologic evaluation in the 174 patients revealed various minor abnormalities in 64; however, in no instance was there significant urologic disease. During follow-up of five to 23 years in 150 of the 174 patients, 106 had no further hematospermia whereas 44 had recurring hematospermia, which persisted in some for more than ten years. None of these patients developed any significant disease related to the hematospermia. In our experience, the occurrence of hematospermia in an otherwise asymptomatic man with normal findings on physical examination, including digital rectal examination, seems not to be associated with the presence of, and does not lead to future development of, significant urologic disease.", "contents": "Hematospermia. During a 15-year period, 200 patients with hematospermia were seen at the Mayo Clinic. General physical examination, including digital rectal palpation and urinalysis, was performed on all 200 patients. Of these, 26 had no additional urologic evaluation. In the remaining 174 patients, cystoscopy and K.U.B. roentgenography were done. The results of urologic evaluation in the 174 patients revealed various minor abnormalities in 64; however, in no instance was there significant urologic disease. During follow-up of five to 23 years in 150 of the 174 patients, 106 had no further hematospermia whereas 44 had recurring hematospermia, which persisted in some for more than ten years. None of these patients developed any significant disease related to the hematospermia. In our experience, the occurrence of hematospermia in an otherwise asymptomatic man with normal findings on physical examination, including digital rectal examination, seems not to be associated with the presence of, and does not lead to future development of, significant urologic disease."}
{"id": "PMID:1097580", "title": "A critical review of periodic health screening using specific screening criteria. 3. Selected diseases of the genitourinary system.", "content": "Despite the increasing interest in recent years in prevention and early recognition of asymptomatic disease, an objectively based program for periodic health screening of asymptomatic adults has yet to be proposed for the primary care physician. This is the third in a series of four articles which will critically examine the feasibility of screening procedures for 36 selected diseases. Six basic criteria are adopted as necessary to justify periodic screening. Specific screening recommendations are made for each disease, and a longitudinal screening program for asymptomatic adults will be proposed in the concluding article of this series.", "contents": "A critical review of periodic health screening using specific screening criteria. 3. Selected diseases of the genitourinary system. Despite the increasing interest in recent years in prevention and early recognition of asymptomatic disease, an objectively based program for periodic health screening of asymptomatic adults has yet to be proposed for the primary care physician. This is the third in a series of four articles which will critically examine the feasibility of screening procedures for 36 selected diseases. Six basic criteria are adopted as necessary to justify periodic screening. Specific screening recommendations are made for each disease, and a longitudinal screening program for asymptomatic adults will be proposed in the concluding article of this series."}
{"id": "PMID:1097581", "title": "A synthetic strand of cardiac muscle: its passive electrical properties.", "content": "The passive electrical properties of synthetic strands of cardiac muscle, grown in tissue culture, were studied using two intracellular microelectrodes: one to inject a rectangular pulse of current and the other to record the resultant displacement of membrane potential at various distances from the current source. In all preparations, the potential displacement, instead of approaching a steady value as would be expected for a cell with constant electrical properties, increased slowly with time throughout the current step. In such circumstances, the specific electrical constants for the membrane and cytoplasm must not be obtained by applying the usual methods, which are based on the analytical solution of the partial differential equation describing a one-dimensional cell with constant electrical properties. A satisfactory fit of the potential waveforms was, however, obtained with numerical solutions of a modified form of this equation in which the membrane resistance increased linearly with time. Best fits of the waveforms from 12 preparations gave the following values for the membrane resistance times unit length, membrane capacitance per unit length, and for the myoplasmic resistance: 1.22 plus or minus 0.13 x 10-5 omegacm, 0.224 plus or minus 0.023 uF with cm-minus 1, and 1.37 plus or minus 0.13 x 10-7 omegacm-minus 1, respectively. The value of membrane capacitance per unit length was close to that obtained from the time constant of the foot of the action potential and was in keeping with the generally satisfactory fit of the recorded waveforms with solutions of the cable equation in which the membrane impedance is that of a single capacitor and resistor in parallel. The area of membrane per unit length and the cross-sectional area of myoplasm at any given length of the preparation were determined from light and composite electron micrographs, and these were used to calculate the following values for the specific electrical membrane resistance, membrane capacitance, and the resistivity of the cytoplasm: 20.5 plus or minus 3.0 x 10-3 omegacm-2, l.54 plus or minus 0.24 uFWITHcm-minus 2, and 180 plus or minus 34 omegacm, respectively.", "contents": "A synthetic strand of cardiac muscle: its passive electrical properties. The passive electrical properties of synthetic strands of cardiac muscle, grown in tissue culture, were studied using two intracellular microelectrodes: one to inject a rectangular pulse of current and the other to record the resultant displacement of membrane potential at various distances from the current source. In all preparations, the potential displacement, instead of approaching a steady value as would be expected for a cell with constant electrical properties, increased slowly with time throughout the current step. In such circumstances, the specific electrical constants for the membrane and cytoplasm must not be obtained by applying the usual methods, which are based on the analytical solution of the partial differential equation describing a one-dimensional cell with constant electrical properties. A satisfactory fit of the potential waveforms was, however, obtained with numerical solutions of a modified form of this equation in which the membrane resistance increased linearly with time. Best fits of the waveforms from 12 preparations gave the following values for the membrane resistance times unit length, membrane capacitance per unit length, and for the myoplasmic resistance: 1.22 plus or minus 0.13 x 10-5 omegacm, 0.224 plus or minus 0.023 uF with cm-minus 1, and 1.37 plus or minus 0.13 x 10-7 omegacm-minus 1, respectively. The value of membrane capacitance per unit length was close to that obtained from the time constant of the foot of the action potential and was in keeping with the generally satisfactory fit of the recorded waveforms with solutions of the cable equation in which the membrane impedance is that of a single capacitor and resistor in parallel. The area of membrane per unit length and the cross-sectional area of myoplasm at any given length of the preparation were determined from light and composite electron micrographs, and these were used to calculate the following values for the specific electrical membrane resistance, membrane capacitance, and the resistivity of the cytoplasm: 20.5 plus or minus 3.0 x 10-3 omegacm-2, l.54 plus or minus 0.24 uFWITHcm-minus 2, and 180 plus or minus 34 omegacm, respectively."}
{"id": "PMID:1097583", "title": "Missense suppression in Coprinus lagopus associated wtih a chromosome duplication.", "content": "Amongst some 70 recessive suppressors of a met-I mutation in Coprinus lagopus, one unstable suppressor was identified. The unstable suppressor, designated sup-6plus, could be maintained on minimal medium, but was lost within 24h on minimal medium containing more than 1-7 p.p.m. DL-methionine or 0-75 p.p.m. L-methionine. Isolation of hyphal tips from the monokaryotic strain carrying sup-6plus yielded three types of colony: the unstable parental type, the stable met-I auxotroph and a stable prototroph which was slow-growing and inhibited by methionine in the growth medium. This stable sup-6plus type was recovered with difficulty by resolving dikaryons formed between the unstable sup-6plus strain and strains carring the wild-type allele of the suppressor gene. From sexual crosses, neither the unstable nor stable sup-6plus type segregated, only the met-I auxotrophic revertant. The unstable sup-6plus strain is thought to have an extra chromosome carrying the sup-6plus mutation. For vigorous growth the wild-type allele, sup-6, is indispensable and would be carried on the homologous chromosome. The selective pressures on different media account for loss of the duplicated chromosomes. The results are interpreted as missense suppression by a mutant of an indispensable tRNA.", "contents": "Missense suppression in Coprinus lagopus associated wtih a chromosome duplication. Amongst some 70 recessive suppressors of a met-I mutation in Coprinus lagopus, one unstable suppressor was identified. The unstable suppressor, designated sup-6plus, could be maintained on minimal medium, but was lost within 24h on minimal medium containing more than 1-7 p.p.m. DL-methionine or 0-75 p.p.m. L-methionine. Isolation of hyphal tips from the monokaryotic strain carrying sup-6plus yielded three types of colony: the unstable parental type, the stable met-I auxotroph and a stable prototroph which was slow-growing and inhibited by methionine in the growth medium. This stable sup-6plus type was recovered with difficulty by resolving dikaryons formed between the unstable sup-6plus strain and strains carring the wild-type allele of the suppressor gene. From sexual crosses, neither the unstable nor stable sup-6plus type segregated, only the met-I auxotrophic revertant. The unstable sup-6plus strain is thought to have an extra chromosome carrying the sup-6plus mutation. For vigorous growth the wild-type allele, sup-6, is indispensable and would be carried on the homologous chromosome. The selective pressures on different media account for loss of the duplicated chromosomes. The results are interpreted as missense suppression by a mutant of an indispensable tRNA."}
{"id": "PMID:1097584", "title": "A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et. sp. nov.", "content": "A new species of nocardioform actinomycete isolated from spontaneously heated sugar-cane bagasse is described as Saccharopolyspora hirsuta gen. et sp. nov. It has affinities with species of both Nocardia and Actinomadura but can be distinguished from both genera by its morphology, sporulation, wall and lipid analyses, antibiotic resistance, degradation and carbon utilzation tests.", "contents": "A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et. sp. nov. A new species of nocardioform actinomycete isolated from spontaneously heated sugar-cane bagasse is described as Saccharopolyspora hirsuta gen. et sp. nov. It has affinities with species of both Nocardia and Actinomadura but can be distinguished from both genera by its morphology, sporulation, wall and lipid analyses, antibiotic resistance, degradation and carbon utilzation tests."}
{"id": "PMID:1097585", "title": "Purification and Characterization of two bacteriocins from Streptococcus faecium.", "content": "Two bacteriocins were found in the supernatant fluid and in an extract of Streptococcus faecium strain EI. The small soluble enterocin EIA represented more than 90% of the total activity in the supernatant fluid, and was purified 400-fold by ammonium sulphate fractionation, gel filtration on Sephadex G-75 and chromatography on DEAE-cellulose. Enterocin EIB, with a particle weight greater than 4 x 10(6), was the predominant type in the extract. It was released in appreciable quantities after breakage of the bacteria and was purified 100-fold by differential centrifugation, chromatography on Sepharose 4B and density gradient ultracentrifugation. Enterocin EIA, a basic substance with a molecular weight of about 10000, was resistant to heat and was attacked by trypsin, whereas enterocin EIB was less thermostable and insensitive to proteolytic enzymes. The activity of enterocin EIB was unchanged by treatment with DNAase. Sensitivity to enterocin action was confined to certain strains of various enterococcus species, Streptococcus salivarius and Listeria monocytogenes; all the other Gram-positive and Gram-negative bacteria tested for sensitivity were unaffected by either enterocin.", "contents": "Purification and Characterization of two bacteriocins from Streptococcus faecium. Two bacteriocins were found in the supernatant fluid and in an extract of Streptococcus faecium strain EI. The small soluble enterocin EIA represented more than 90% of the total activity in the supernatant fluid, and was purified 400-fold by ammonium sulphate fractionation, gel filtration on Sephadex G-75 and chromatography on DEAE-cellulose. Enterocin EIB, with a particle weight greater than 4 x 10(6), was the predominant type in the extract. It was released in appreciable quantities after breakage of the bacteria and was purified 100-fold by differential centrifugation, chromatography on Sepharose 4B and density gradient ultracentrifugation. Enterocin EIA, a basic substance with a molecular weight of about 10000, was resistant to heat and was attacked by trypsin, whereas enterocin EIB was less thermostable and insensitive to proteolytic enzymes. The activity of enterocin EIB was unchanged by treatment with DNAase. Sensitivity to enterocin action was confined to certain strains of various enterococcus species, Streptococcus salivarius and Listeria monocytogenes; all the other Gram-positive and Gram-negative bacteria tested for sensitivity were unaffected by either enterocin."}
{"id": "PMID:1097586", "title": "Extremely thermophilic gram-negative bacteria from hot tap water.", "content": "Two strains of heterotrophic, non-motile, Gram-negative extreme thermophiles have been isolated from hot tap water. These strains (NH and DI) have been characterized and compared with strains of genus Thermus. Few of the single organic compounds tested supported growth in the presence of ammonium salts, and, like Thermus strains, growth on undefined media was restricted to dilute tryptone-yeast extract-mineral salt solutions. Nutrients agar and similar common laboratory media did not support growth. The growth rate was similar to that of Thermus strains, as was the unusual pattern of antibiotic resistance. Mean base composition of NH was 61-4% guanine plus cytosine (G+C), and DI was 62-0 to 62-2% G+C, which are several per cent lower than  the G+C contents of other strains so far described.", "contents": "Extremely thermophilic gram-negative bacteria from hot tap water. Two strains of heterotrophic, non-motile, Gram-negative extreme thermophiles have been isolated from hot tap water. These strains (NH and DI) have been characterized and compared with strains of genus Thermus. Few of the single organic compounds tested supported growth in the presence of ammonium salts, and, like Thermus strains, growth on undefined media was restricted to dilute tryptone-yeast extract-mineral salt solutions. Nutrients agar and similar common laboratory media did not support growth. The growth rate was similar to that of Thermus strains, as was the unusual pattern of antibiotic resistance. Mean base composition of NH was 61-4% guanine plus cytosine (G+C), and DI was 62-0 to 62-2% G+C, which are several per cent lower than  the G+C contents of other strains so far described."}
{"id": "PMID:1097587", "title": "Modification of the membrane composition of Mycoplasma mycoides subsp. capri by the growth medium.", "content": "Mycoplasma mycoides subsp. capri was grown in different media. The amounts of the lipids in these media were varied, resulting in altered lipid compositions of the cells. Lowering the amounts of cholesterol in the media resulted in less cholesterol incorporation into the cell lipid, with a concomitant decrease in the amount of phospholipid. The changes in the phospholipid/cholesterol ratio in the mycoplasmas were very small compared with the large changes in the amounts of cholesterol which occurred when the cholesterol content in the medium was altered. Changes in the amounts of glycolipid and glyceride in the cell lipid also resulted from alterations of the cholesterol concentration in the media. Under these conditions cell with reduced cholesterol contents were more sensitive to lysis by digitonin. No changes were observed in the polyacrylamide gel electrophoretic pattern of cellular or membrane proteins when the cholesterol was replaced by other sterols.", "contents": "Modification of the membrane composition of Mycoplasma mycoides subsp. capri by the growth medium. Mycoplasma mycoides subsp. capri was grown in different media. The amounts of the lipids in these media were varied, resulting in altered lipid compositions of the cells. Lowering the amounts of cholesterol in the media resulted in less cholesterol incorporation into the cell lipid, with a concomitant decrease in the amount of phospholipid. The changes in the phospholipid/cholesterol ratio in the mycoplasmas were very small compared with the large changes in the amounts of cholesterol which occurred when the cholesterol content in the medium was altered. Changes in the amounts of glycolipid and glyceride in the cell lipid also resulted from alterations of the cholesterol concentration in the media. Under these conditions cell with reduced cholesterol contents were more sensitive to lysis by digitonin. No changes were observed in the polyacrylamide gel electrophoretic pattern of cellular or membrane proteins when the cholesterol was replaced by other sterols."}
{"id": "PMID:1097590", "title": "Genetics of a primaquin-resistant yeast.", "content": "Primaquin specifically inhibits mitochondrial function in yeast. Mutants resistant to primaquin have been isolated. Genetic analysis revealed that the expression of resistance in one of them was under the control of both a nuclear gene and a cytoplasmic factor (possibly a mitochondrial gene).", "contents": "Genetics of a primaquin-resistant yeast. Primaquin specifically inhibits mitochondrial function in yeast. Mutants resistant to primaquin have been isolated. Genetic analysis revealed that the expression of resistance in one of them was under the control of both a nuclear gene and a cytoplasmic factor (possibly a mitochondrial gene)."}
{"id": "PMID:1097591", "title": "Sex pili as immunogens.", "content": "Groups of chickens were vaccinated intravenously with live cultures of Escherichia coli K12 possessing transfer factors F, I, A2 or no transfer factor (Tra-minus) and their immunity challenged by injecting them intravenously with an F-plus strain of Salmonella heidelberg or an F-plus or I-plus strain of Salmonella typhimurium. Compared with the Tra-minus strain, vaccination with the F-plus, but not the I-plus or A2-plus, K12 strain significantly reduced the mortality rate caused by the F-plus salmonella strains; vaccination with the I-plus or A2-plus, but not the F-plus, K12 strain significantly reduced the mortality rate caused by the I-plus salmonella strain.", "contents": "Sex pili as immunogens. Groups of chickens were vaccinated intravenously with live cultures of Escherichia coli K12 possessing transfer factors F, I, A2 or no transfer factor (Tra-minus) and their immunity challenged by injecting them intravenously with an F-plus strain of Salmonella heidelberg or an F-plus or I-plus strain of Salmonella typhimurium. Compared with the Tra-minus strain, vaccination with the F-plus, but not the I-plus or A2-plus, K12 strain significantly reduced the mortality rate caused by the F-plus salmonella strains; vaccination with the I-plus or A2-plus, but not the F-plus, K12 strain significantly reduced the mortality rate caused by the I-plus salmonella strain."}
{"id": "PMID:1097592", "title": "A comparison of analytical methods for the numerical taxonomy of yeasts.", "content": "Four methods of numerical analysis were tested to assess the taxonomic relationships of species of yeasts in 20 genera using characters obtained from standard descriptions. Relationships varied according to the analytical method employed. It is suggested that a reduction in the present number of genera may be necessary to justify the taxonomic rank of genus.", "contents": "A comparison of analytical methods for the numerical taxonomy of yeasts. Four methods of numerical analysis were tested to assess the taxonomic relationships of species of yeasts in 20 genera using characters obtained from standard descriptions. Relationships varied according to the analytical method employed. It is suggested that a reduction in the present number of genera may be necessary to justify the taxonomic rank of genus."}
{"id": "PMID:1097595", "title": "Genetical studies of serum resistance in Escherichia coli.", "content": "One induced and one spontaneous serum-resistant mutant were derived from smooth serum-sensitive Escherichia coli strains. There was little difference in the yield or the O-side-chain-sugar to core-sugar ratio of lipopolysaccharides from the mutants compared with the parental strains. The mutations to serum resistance were not accompanied by increases in either the amount or haemagglutination-inhibiting activity of acidic polysaccharides extracted from the strains. Two colonially rough forms, designated 17fa and 17gb, were isolated from an aged culture of serum-resistant mutant 17. Escherichia coli 17fa appeared to be a som mutant and was rapidly killed by serum. Escherichia coli 17gb retained serological O-specificity, and 17gb lipopolysaccharide contained a full complement of O-side-chains; the strain was killed by serum but only after a delay of 1 h. Escherichia coli K-negative O8 donor Hfr59, which was serum-resistant, was crossed with rough serum-sensitive E. coli strain F470 and his+ recombinants were selected. The majority of his-+ recombinants inherited a full complement of lipopolysaccharide O-side-chains but were killed by serum after a delay of 1 to 2 h. These results suggest that lipopolysaccharide O-side-chains are responsible for a delay in the killing by normal human serum of smooth E. coli strains but that other factors determine full serum resistance. No evidence was found of a role for K-antigens in serum resistance.", "contents": "Genetical studies of serum resistance in Escherichia coli. One induced and one spontaneous serum-resistant mutant were derived from smooth serum-sensitive Escherichia coli strains. There was little difference in the yield or the O-side-chain-sugar to core-sugar ratio of lipopolysaccharides from the mutants compared with the parental strains. The mutations to serum resistance were not accompanied by increases in either the amount or haemagglutination-inhibiting activity of acidic polysaccharides extracted from the strains. Two colonially rough forms, designated 17fa and 17gb, were isolated from an aged culture of serum-resistant mutant 17. Escherichia coli 17fa appeared to be a som mutant and was rapidly killed by serum. Escherichia coli 17gb retained serological O-specificity, and 17gb lipopolysaccharide contained a full complement of O-side-chains; the strain was killed by serum but only after a delay of 1 h. Escherichia coli K-negative O8 donor Hfr59, which was serum-resistant, was crossed with rough serum-sensitive E. coli strain F470 and his+ recombinants were selected. The majority of his-+ recombinants inherited a full complement of lipopolysaccharide O-side-chains but were killed by serum after a delay of 1 to 2 h. These results suggest that lipopolysaccharide O-side-chains are responsible for a delay in the killing by normal human serum of smooth E. coli strains but that other factors determine full serum resistance. No evidence was found of a role for K-antigens in serum resistance."}
{"id": "PMID:1097596", "title": "Therapeutic reversal with benxtropine in schizophrenics. Practical and theoretical significance.", "content": "The effect of an anticholinergic antiparkinsonism drug, benztropine, on the therapeutic course of neuroleptic treatment in 18 schizophrenics was investigated in a double blind cross-over study involving haloperidol and chlorpromazine. Significant therapeutic reversal was observed with benztropine in terms of the social, affective,and cognitive dysfunctions chracteristically seen in schizophrenic psychosis. The hallucinatory behavior and disturbed attention were not so affected. The aspects of the clinical picture to show significant nontherapeutic change with benxtropine differed with the stage of treatment and seemed to be determined by the kinetics of the therapeutic process. The effect was one of exacerbation of the disorder and not a toxic confusional state sometimes associated with anticholinergic drugs. The practical and theoretical significance of these findings was discussed. It was suggested that the benztropine reversal of therapeutic changes provided a valuable pharmacological model for understanding the neurobiological basis of schizophrenic decompensation and its restitution with neuroleptics. The reported data were considered as indirect evidence suggesting that cholinergic neuronal mechnisms are involved in both of these processes. It was speculated that these mechanisms may well be the cholinergic suppressor systems, such as the periventricular catecholamine pathways in the limbic organization and bsal ganglia known to be affected by neuroleptics.", "contents": "Therapeutic reversal with benxtropine in schizophrenics. Practical and theoretical significance. The effect of an anticholinergic antiparkinsonism drug, benztropine, on the therapeutic course of neuroleptic treatment in 18 schizophrenics was investigated in a double blind cross-over study involving haloperidol and chlorpromazine. Significant therapeutic reversal was observed with benztropine in terms of the social, affective,and cognitive dysfunctions chracteristically seen in schizophrenic psychosis. The hallucinatory behavior and disturbed attention were not so affected. The aspects of the clinical picture to show significant nontherapeutic change with benxtropine differed with the stage of treatment and seemed to be determined by the kinetics of the therapeutic process. The effect was one of exacerbation of the disorder and not a toxic confusional state sometimes associated with anticholinergic drugs. The practical and theoretical significance of these findings was discussed. It was suggested that the benztropine reversal of therapeutic changes provided a valuable pharmacological model for understanding the neurobiological basis of schizophrenic decompensation and its restitution with neuroleptics. The reported data were considered as indirect evidence suggesting that cholinergic neuronal mechnisms are involved in both of these processes. It was speculated that these mechanisms may well be the cholinergic suppressor systems, such as the periventricular catecholamine pathways in the limbic organization and bsal ganglia known to be affected by neuroleptics."}
{"id": "PMID:1097597", "title": "The effects of electrosleep on insomnia revisited.", "content": "Ten subjects who had suffered from sleep onset insomnia for a minimun of 2 years participated in a double blind study of the effects of electrosleep on this disorder.  This paper reports a 2-year follow-up of these subjects.  Of the five subjects who received 24 live treatments, four appeared to be able to fall asleep with little difficulty and to awake feeling moderately to very well rested.  Only one appeared to have relapsed during the 2-year-no-treatment period.  Of those receiving sham treatment four were having quite a bit of difficulty falling asleep but three of the five awoke feeling moderately well rested.  Although the number of subjects is small, the trends appear consistent with the interpretation that sleep habits were improved for most of the real treatment subjects and for few of those receiving sham treatment.", "contents": "The effects of electrosleep on insomnia revisited. Ten subjects who had suffered from sleep onset insomnia for a minimun of 2 years participated in a double blind study of the effects of electrosleep on this disorder.  This paper reports a 2-year follow-up of these subjects.  Of the five subjects who received 24 live treatments, four appeared to be able to fall asleep with little difficulty and to awake feeling moderately to very well rested.  Only one appeared to have relapsed during the 2-year-no-treatment period.  Of those receiving sham treatment four were having quite a bit of difficulty falling asleep but three of the five awoke feeling moderately well rested.  Although the number of subjects is small, the trends appear consistent with the interpretation that sleep habits were improved for most of the real treatment subjects and for few of those receiving sham treatment."}
{"id": "PMID:1097598", "title": "Plasma concentrations of L-dopa and 3-methoxydopa and improvement in clinical ratings and motor performance in patients with Parkinsonism treated with L-dopa alone or in combination with amantadine.", "content": "Six patients with idiopathic Parkinsonism were treated with a combination of amantadine and L-dopa and after 12 to 24 weeks amantadine was replaced by placebo for a six week period in a double-blind trial. Although there was a tendency for clinical disability ratings and scores on objective ratings of motor skills to deteriorate initially after amantadine removal, there was no significant deterioration in clinical improvement or motor performance during the period of amantadine withdrawal. Amantadine withdrawal also failed to cause any significant change in plasma concentrations of L-dopa or its metabolite 3-methoxy-dopa in these patients. In a group of 27 patients seen regularly as outpatients measurements of plasma L-dopa failed to correlate significantly with either oral dose or with clinical improvement scores. The plasma concentration of 3-methoxy-dopa, however, was on average 2.8 times higher than that of L-dopa, and there was a significant correlation between plasma levels of this metabolite and clinical improvement. It is suggested that 3-methoxy-dopa may contribute significantly to the therapeutic actions of L-dopa in Parkinsonism.", "contents": "Plasma concentrations of L-dopa and 3-methoxydopa and improvement in clinical ratings and motor performance in patients with Parkinsonism treated with L-dopa alone or in combination with amantadine. Six patients with idiopathic Parkinsonism were treated with a combination of amantadine and L-dopa and after 12 to 24 weeks amantadine was replaced by placebo for a six week period in a double-blind trial. Although there was a tendency for clinical disability ratings and scores on objective ratings of motor skills to deteriorate initially after amantadine removal, there was no significant deterioration in clinical improvement or motor performance during the period of amantadine withdrawal. Amantadine withdrawal also failed to cause any significant change in plasma concentrations of L-dopa or its metabolite 3-methoxy-dopa in these patients. In a group of 27 patients seen regularly as outpatients measurements of plasma L-dopa failed to correlate significantly with either oral dose or with clinical improvement scores. The plasma concentration of 3-methoxy-dopa, however, was on average 2.8 times higher than that of L-dopa, and there was a significant correlation between plasma levels of this metabolite and clinical improvement. It is suggested that 3-methoxy-dopa may contribute significantly to the therapeutic actions of L-dopa in Parkinsonism."}
{"id": "PMID:1097599", "title": "Epidemiology of Creutzfeldt-Jakob disease in England and Wales.", "content": "Some aspects of the epidemiology of Creutzfeldt-Jakob disease in England and Wales in the decade 1964-73 were studied with the object of detecting evidence of natural transmission of this slow virus encephalopathy. Some geographical clustering and possibility of contact between cases was found.", "contents": "Epidemiology of Creutzfeldt-Jakob disease in England and Wales. Some aspects of the epidemiology of Creutzfeldt-Jakob disease in England and Wales in the decade 1964-73 were studied with the object of detecting evidence of natural transmission of this slow virus encephalopathy. Some geographical clustering and possibility of contact between cases was found."}
{"id": "PMID:1097600", "title": "Amphetamines in the treatment of Parkinson's disease.", "content": "Twenty-two patients with Parkinsonism were treated with levoamphetamine and 12 of these with dextroamphetamine. Levoamphetamine resulted in a significant improvement in disability from Parkinsonism, although the reduction in total disability, tremor, akinesia, and rigidity scores was slight (ca 20 percent). Dextroamphetamine in lower dosage also reduced disability by some 17 percent. The most disabled patients, including those also on levodopa, showed the greatest response to amphetamines. Previously, amphetamines have been reported to be a selective treatment for the oculogyric crises of post-encephalitic Parkinsonism. Amphetamines are thought to cause the release of catecholamines from central neurones. Their action in Parkinson's disease may be limited because of pre-existing striatal dopamine deficiency. Side-effects of amphetamines, anorexia, and CNS stimulation are different from those caused by levodopa in patients with Parkinson's disease.", "contents": "Amphetamines in the treatment of Parkinson's disease. Twenty-two patients with Parkinsonism were treated with levoamphetamine and 12 of these with dextroamphetamine. Levoamphetamine resulted in a significant improvement in disability from Parkinsonism, although the reduction in total disability, tremor, akinesia, and rigidity scores was slight (ca 20 percent). Dextroamphetamine in lower dosage also reduced disability by some 17 percent. The most disabled patients, including those also on levodopa, showed the greatest response to amphetamines. Previously, amphetamines have been reported to be a selective treatment for the oculogyric crises of post-encephalitic Parkinsonism. Amphetamines are thought to cause the release of catecholamines from central neurones. Their action in Parkinson's disease may be limited because of pre-existing striatal dopamine deficiency. Side-effects of amphetamines, anorexia, and CNS stimulation are different from those caused by levodopa in patients with Parkinson's disease."}
{"id": "PMID:1097601", "title": "Intraventricular cryptococcal granuloma.", "content": "A case is reported of a cryptococcal granuloma occurring within the lateral ventricle. The findings on angiography and brain-scanning led to a preoperative diagnosis of intraventricular meningioma. There are no previous reports of an isotope brain-scan in this condition and angiography usually shows an avascular swelling.", "contents": "Intraventricular cryptococcal granuloma. A case is reported of a cryptococcal granuloma occurring within the lateral ventricle. The findings on angiography and brain-scanning led to a preoperative diagnosis of intraventricular meningioma. There are no previous reports of an isotope brain-scan in this condition and angiography usually shows an avascular swelling."}
{"id": "PMID:1097602", "title": "Clinical syndromes of arteriovenous malformations of the transverse-sigmoid sinus.", "content": "Arteriovenous malformations or fistulae shunting arterial blood from branches of the external and internal carotid and vertebral arteries into the transverse-sigmoid sinus may produce different clinical syndromes. The literature is reviewed with 96 patients including six personal cases. Usually these malformations have a congenital origin and only in 4% of the series was there a previous history of a severe head injury. Clinical groups are defined and the role of angiography assessed. Direct surgical approach with occlusion or removal of the vascular malformation is the treatment of choice. Possible methods of treatment by selective embolization are discussed.", "contents": "Clinical syndromes of arteriovenous malformations of the transverse-sigmoid sinus. Arteriovenous malformations or fistulae shunting arterial blood from branches of the external and internal carotid and vertebral arteries into the transverse-sigmoid sinus may produce different clinical syndromes. The literature is reviewed with 96 patients including six personal cases. Usually these malformations have a congenital origin and only in 4% of the series was there a previous history of a severe head injury. Clinical groups are defined and the role of angiography assessed. Direct surgical approach with occlusion or removal of the vascular malformation is the treatment of choice. Possible methods of treatment by selective embolization are discussed."}
{"id": "PMID:1097603", "title": "A phenothiazine derivative in the treatment of spasticity.", "content": "The efficacy of a selective fusimotor suppressant, the phenothiazine (+/-)-10-3-dimethylamino-2-methylpropyl)-2-valeroylphenothiazine, has been assessed in a double-blind crossover trail in eight patients suffering from cerebral spasticity and one patient suffering from spinal spasticity. Dosage was 40 mg daily. Independent clinical and electromyographic methods of assessment were used. The active agent produced a small but significant reduction in spasticity, although this was of clinical value in only a few patients. There were few side-effects. It is recommended that further studies using higher dosages be undertaken.", "contents": "A phenothiazine derivative in the treatment of spasticity. The efficacy of a selective fusimotor suppressant, the phenothiazine (+/-)-10-3-dimethylamino-2-methylpropyl)-2-valeroylphenothiazine, has been assessed in a double-blind crossover trail in eight patients suffering from cerebral spasticity and one patient suffering from spinal spasticity. Dosage was 40 mg daily. Independent clinical and electromyographic methods of assessment were used. The active agent produced a small but significant reduction in spasticity, although this was of clinical value in only a few patients. There were few side-effects. It is recommended that further studies using higher dosages be undertaken."}
{"id": "PMID:1097604", "title": "Cyclophosphamide in exacerbations of multiple sclerosis. Therapeutic trial and a strategy for pilot drug studies.", "content": "Cyclophosphamide (CY) has been shown to reverse the signs of experimental allergic encephalomyelitis (EAE) even after the onset of neurological deficits. Because of the analogy of EAE to exacerbations of multiple sclerosis (MS) a clinical trial of CY in acute MS exacerbations was undertaken. A 'sequential criterion' method was used to minimize the size of sample needed for this pilot study. CY failed to alter significantly the course of acute exacerbations of MS. Possible reasons for this failure, and the value of the sequential criterion method in pilot studies, are discussed.", "contents": "Cyclophosphamide in exacerbations of multiple sclerosis. Therapeutic trial and a strategy for pilot drug studies. Cyclophosphamide (CY) has been shown to reverse the signs of experimental allergic encephalomyelitis (EAE) even after the onset of neurological deficits. Because of the analogy of EAE to exacerbations of multiple sclerosis (MS) a clinical trial of CY in acute MS exacerbations was undertaken. A 'sequential criterion' method was used to minimize the size of sample needed for this pilot study. CY failed to alter significantly the course of acute exacerbations of MS. Possible reasons for this failure, and the value of the sequential criterion method in pilot studies, are discussed."}
{"id": "PMID:1097605", "title": "Cerebral haemorrhages with atypical clinical patterns. A study of cerebral haematomasusing CSF spectrophotometry and computerized transverse axial tomography (\"EMI scanning\").", "content": "Twelve patients with 13 cerebrovascular incidents presenting with atypical clincial patterns where thecombination of CSF  spectrophotometry (CSF-SPE) and computerized transverse axialtomogrpahy(CTAT, \"EMI-scanning\") showed cerebral haematomas, with or without haemorrhage into the CSFare reported. The high diagnostic significance of SDF-SPE as well as the value of using a combination of CSF-SPE and CTAT examinations in cerebrovascular disease has preveiouslybeen  established by the authors. The present patient group comprised some 10% of a totalseries of patients with cerebrovascular disease hitherto studied in this fashion. Conclusive findings were found in only 1 fo the 5 cases examined by angiography and in none of the5 cases studied by isotope scan. Since many cerebral haemorrhages obviosly haveatypical clincalfeatures as compared with the \"classical\" pattern, the combined use of CSF-SPE and CTAT examinations in the diagnosis of cerebral haematomas is a prereguisite for rational therapy.", "contents": "Cerebral haemorrhages with atypical clinical patterns. A study of cerebral haematomasusing CSF spectrophotometry and computerized transverse axial tomography (\"EMI scanning\"). Twelve patients with 13 cerebrovascular incidents presenting with atypical clincial patterns where thecombination of CSF  spectrophotometry (CSF-SPE) and computerized transverse axialtomogrpahy(CTAT, \"EMI-scanning\") showed cerebral haematomas, with or without haemorrhage into the CSFare reported. The high diagnostic significance of SDF-SPE as well as the value of using a combination of CSF-SPE and CTAT examinations in cerebrovascular disease has preveiouslybeen  established by the authors. The present patient group comprised some 10% of a totalseries of patients with cerebrovascular disease hitherto studied in this fashion. Conclusive findings were found in only 1 fo the 5 cases examined by angiography and in none of the5 cases studied by isotope scan. Since many cerebral haemorrhages obviosly haveatypical clincalfeatures as compared with the \"classical\" pattern, the combined use of CSF-SPE and CTAT examinations in the diagnosis of cerebral haematomas is a prereguisite for rational therapy."}
{"id": "PMID:1097606", "title": "Ligation of the vertebral (unilateral or bilateral) or basilar artery in the treatment of large intracranial aneurysms.", "content": "The author reports the use of vertebral artery ligation, unilateral and bilateral, for the treatment of large vertebral-basilar aneurysms in 14 patients with one delayed death. Extracranial ligation was carried out unilaterally with a Selverstone clamp in three patients. In two, where the aneurysm filled only from one vertebral artery, there was extensive thrombosis within the sac and dramatic clinical improvement after decompression. Extracranial ligation was done bilaterally in three patients, temporarily in two. A 14-year-old boy is well after 5 years but the bilateral vertebrobasilar aneurysm did not undergo extensive thrombosis until both vertebral arteries were occluded at their intracranial entrance above collateral flow. In two others, the clamp had to be reopened on the second artery. In one patient, death from delayed thrombosis of a huge aneurysm and pontine infarction might have been prevented with anticoagulants. In the other, the aneurysm ruptured again fatally 18 months later. Unilateral intracranial occlusion of a vertebral artery was done in eight cases, with no morbidity and complete or nearly complete thrombosis in all but one aneurysm. Seven patients had excellent or good results while one showed little recovery from an existing medullary syndrome. Occlusion of the basilar artery was done in seven cases. In five it was used deliberately as the only treatment, but in two it was forced when an aneurysm burst during dissection. Only two of the patients in the first group and one of the second group have made complete recoveries. The results of vertebral artery occlusion are encouraging and the technique deserves further consideration. Extensive collateral circulation enhances the safety of cervical vertebral artery occlusion but can be of a degree to make the occlusion ineffective. For intracranial occlusion knowledge of the size and distribution of each vertebral artery is essential. Occlusion of the basilar artery is dangerous, although it seems to be effective in producing extensive thrombosis in the aneurysm. It should probably be done under anesthesia only when the artery fills spontaneously from the carotid circulation. Otherwise, even when reasonable posterior communicating arteries are demonstrated, it is best to test occlusion under local anesthesia.", "contents": "Ligation of the vertebral (unilateral or bilateral) or basilar artery in the treatment of large intracranial aneurysms. The author reports the use of vertebral artery ligation, unilateral and bilateral, for the treatment of large vertebral-basilar aneurysms in 14 patients with one delayed death. Extracranial ligation was carried out unilaterally with a Selverstone clamp in three patients. In two, where the aneurysm filled only from one vertebral artery, there was extensive thrombosis within the sac and dramatic clinical improvement after decompression. Extracranial ligation was done bilaterally in three patients, temporarily in two. A 14-year-old boy is well after 5 years but the bilateral vertebrobasilar aneurysm did not undergo extensive thrombosis until both vertebral arteries were occluded at their intracranial entrance above collateral flow. In two others, the clamp had to be reopened on the second artery. In one patient, death from delayed thrombosis of a huge aneurysm and pontine infarction might have been prevented with anticoagulants. In the other, the aneurysm ruptured again fatally 18 months later. Unilateral intracranial occlusion of a vertebral artery was done in eight cases, with no morbidity and complete or nearly complete thrombosis in all but one aneurysm. Seven patients had excellent or good results while one showed little recovery from an existing medullary syndrome. Occlusion of the basilar artery was done in seven cases. In five it was used deliberately as the only treatment, but in two it was forced when an aneurysm burst during dissection. Only two of the patients in the first group and one of the second group have made complete recoveries. The results of vertebral artery occlusion are encouraging and the technique deserves further consideration. Extensive collateral circulation enhances the safety of cervical vertebral artery occlusion but can be of a degree to make the occlusion ineffective. For intracranial occlusion knowledge of the size and distribution of each vertebral artery is essential. Occlusion of the basilar artery is dangerous, although it seems to be effective in producing extensive thrombosis in the aneurysm. It should probably be done under anesthesia only when the artery fills spontaneously from the carotid circulation. Otherwise, even when reasonable posterior communicating arteries are demonstrated, it is best to test occlusion under local anesthesia."}
{"id": "PMID:1097607", "title": "Scintiphotography in diagnosis of urinary fistula after renal transplantation.", "content": "Scintiphotographic studies in six patients with ureteral fistula following renal transplantation are presented. Images were obtained using 99m-Tc-Sn-DTPA or 131-I-orthoiodohippurate. Urinary leakage was accurately detected in each case but the pattern of extravasation is highly variable. When carefully performed, radionuclide scintiphotography is a safe and effective method for detecting urinary leakage after renal transplantation.", "contents": "Scintiphotography in diagnosis of urinary fistula after renal transplantation. Scintiphotographic studies in six patients with ureteral fistula following renal transplantation are presented. Images were obtained using 99m-Tc-Sn-DTPA or 131-I-orthoiodohippurate. Urinary leakage was accurately detected in each case but the pattern of extravasation is highly variable. When carefully performed, radionuclide scintiphotography is a safe and effective method for detecting urinary leakage after renal transplantation."}
{"id": "PMID:1097608", "title": "Effect of obstructed mediastinal venous return on dynamic brain blood flow studies: Case report.", "content": "Three patients are described who had unusual isotope brain flow studies in which the injected isotope refluxed into the internal jugular vein, entered the transverse dural sinuses, and returned proximally to a variable extent through the contralateral internal jugular vein. Mediastinal flow studies revealed obstructed pathways of venous return in the upper mediastinum. One patient had lymphoma, another had had a pneumonectomy, and the third had no demonstrable disease process to explain the flow pattern. The internal jugular-cerebral system apparently permits reversal of flow in the presence of unilateral obstruction proximal to the internal jugular vein.", "contents": "Effect of obstructed mediastinal venous return on dynamic brain blood flow studies: Case report. Three patients are described who had unusual isotope brain flow studies in which the injected isotope refluxed into the internal jugular vein, entered the transverse dural sinuses, and returned proximally to a variable extent through the contralateral internal jugular vein. Mediastinal flow studies revealed obstructed pathways of venous return in the upper mediastinum. One patient had lymphoma, another had had a pneumonectomy, and the third had no demonstrable disease process to explain the flow pattern. The internal jugular-cerebral system apparently permits reversal of flow in the presence of unilateral obstruction proximal to the internal jugular vein."}
{"id": "PMID:1097609", "title": "The bonding of a polymeric fissure sealant to topical fluoride-treated teeth.", "content": "The tensile bond strength of a polymeric fissure sealant to enamel treated with a variety of topical fluorides was determined. Application of acidulated phosphate fluoride solutions (APF) prior to and after acid etching reduces the tensile bond strength of Nuva Seal from 36-6+/-9-0 kg/cm2 to 4-9+/-3-3 kg/cm2 and 23-8+/-6-3 kg/cm2 respectively. The treatment of the etched enamel with freshly prepared 8% stannous fluoride solution prior to Nuva Seal application significantly increases the tensile bond strength to 55-7+/-17-1 kg/cm2. It is recommended that clinical procedures such as the application of fissure sealants, acid etch composite restorations and the direct attachment of orthodontic brackets, all of which involve etching of the enamel, should not be precedded by the topical application of APF solutions.", "contents": "The bonding of a polymeric fissure sealant to topical fluoride-treated teeth. The tensile bond strength of a polymeric fissure sealant to enamel treated with a variety of topical fluorides was determined. Application of acidulated phosphate fluoride solutions (APF) prior to and after acid etching reduces the tensile bond strength of Nuva Seal from 36-6+/-9-0 kg/cm2 to 4-9+/-3-3 kg/cm2 and 23-8+/-6-3 kg/cm2 respectively. The treatment of the etched enamel with freshly prepared 8% stannous fluoride solution prior to Nuva Seal application significantly increases the tensile bond strength to 55-7+/-17-1 kg/cm2. It is recommended that clinical procedures such as the application of fissure sealants, acid etch composite restorations and the direct attachment of orthodontic brackets, all of which involve etching of the enamel, should not be precedded by the topical application of APF solutions."}
{"id": "PMID:1097610", "title": "Relationship of the maxillary central incisors and canines to the incisive papilla.", "content": "Four hundred and thirty dentulous casts in normal alignment and Angle's Class I Relationship were examined. The results indicated a relationship between arch shape and the incisive papilla. The suggestion is made that in the preliminary location of the anterior teeth during construction of full dentures, the average distance of incisors and canines from the incisive papilla could be used as starting points.", "contents": "Relationship of the maxillary central incisors and canines to the incisive papilla. Four hundred and thirty dentulous casts in normal alignment and Angle's Class I Relationship were examined. The results indicated a relationship between arch shape and the incisive papilla. The suggestion is made that in the preliminary location of the anterior teeth during construction of full dentures, the average distance of incisors and canines from the incisive papilla could be used as starting points."}
{"id": "PMID:1097611", "title": "Carisoprodol in the treatment of myofascial pain-dysfunction syndrome.", "content": "The effects of carisoprodol and a placebo were compared in a sample of 60 patients with the clinical diagnosis of myofascial pain-dysfunction syndrome using double-blind design.  Carisoprodol failed to show a significantly greater effect than the placebo.  The side effects were about the same with both agents.", "contents": "Carisoprodol in the treatment of myofascial pain-dysfunction syndrome. The effects of carisoprodol and a placebo were compared in a sample of 60 patients with the clinical diagnosis of myofascial pain-dysfunction syndrome using double-blind design.  Carisoprodol failed to show a significantly greater effect than the placebo.  The side effects were about the same with both agents."}
{"id": "PMID:1097612", "title": "The testis in mice infected with Mycobacterium leprae.", "content": "Following inoculation either locally or intravenously with Mycobact. leprae of human origin, the histopathology and bacteriology of the testis in experimental mice is described. Normal mice, and mice rendered immunologically deficient by thymectomy and whole-body irradiation, were studied. Attention is drawn to a heavy bacillation of the testis in mice from both groups. Bacilli were found in and beneath the tunica albuginea, but mainly in interstitial cells and in macrophages surrounding the tubules. The percentage of solidly staining bacilli was high, and globi were frequent. The study showed that the testis in mice is particularly favourable for the lodgement and multiplication of Mycobact. laprae following either local or intravenous inoculation. The significance of this in relation to the metabolism of the leprosy bacillus and to the frequent occurrence of testicular damage in the lepromatous male patient is discussed. This work was supported by grants to A. G. M. Weddell and A. C. McDougall from the Medical Research Council and the British Leprosy Relief Association (LEPRA).", "contents": "The testis in mice infected with Mycobacterium leprae. Following inoculation either locally or intravenously with Mycobact. leprae of human origin, the histopathology and bacteriology of the testis in experimental mice is described. Normal mice, and mice rendered immunologically deficient by thymectomy and whole-body irradiation, were studied. Attention is drawn to a heavy bacillation of the testis in mice from both groups. Bacilli were found in and beneath the tunica albuginea, but mainly in interstitial cells and in macrophages surrounding the tubules. The percentage of solidly staining bacilli was high, and globi were frequent. The study showed that the testis in mice is particularly favourable for the lodgement and multiplication of Mycobact. laprae following either local or intravenous inoculation. The significance of this in relation to the metabolism of the leprosy bacillus and to the frequent occurrence of testicular damage in the lepromatous male patient is discussed. This work was supported by grants to A. G. M. Weddell and A. C. McDougall from the Medical Research Council and the British Leprosy Relief Association (LEPRA)."}
{"id": "PMID:1097613", "title": "Hyperacute rejection of guinea-pig to rat cardiac xenografts. I. Morphology.", "content": "The morphological changes occurring in xenograft hyperacute rejection, using guinea-pig to rat cardiac transplants, have been studied. Ultrastructural evidence of platelet aggregation in myocardial capillaries and veins first occurred two minutes after revascularisation. Small amounts of fibrin, associated with platelet degranulation occurred at three minutes. From four minutes, platelet aggregates increased in number and endothelial damage first appeared in those vessels containing platelet aggregates. Rejection was complete by 12 minutes and platelet aggregates then filled many capillaries, veins, arterioles and arteries. Polymorphonuclear leucocyte infiltration was not seen.", "contents": "Hyperacute rejection of guinea-pig to rat cardiac xenografts. I. Morphology. The morphological changes occurring in xenograft hyperacute rejection, using guinea-pig to rat cardiac transplants, have been studied. Ultrastructural evidence of platelet aggregation in myocardial capillaries and veins first occurred two minutes after revascularisation. Small amounts of fibrin, associated with platelet degranulation occurred at three minutes. From four minutes, platelet aggregates increased in number and endothelial damage first appeared in those vessels containing platelet aggregates. Rejection was complete by 12 minutes and platelet aggregates then filled many capillaries, veins, arterioles and arteries. Polymorphonuclear leucocyte infiltration was not seen."}
{"id": "PMID:1097614", "title": "Development of preschool-aged children of different social and ethnic groups: implications for developmental screening.", "content": "The developmental status of 1,180 children representing a cross section of Denver's ethnic and parental occupational groups was compared with that of 1,055 children (349 Anglo, 354 Spanish surname, and 352 Black) whose parents were unskilled workers. The children varied in age from 2 weeks to 6.4 years and were evaluated with the Denver developmental screening test. Comparisons were made in developmental achievements as reflected by individual item differences for Anglo children from unskilled families with Anglos from cross-sectional families. A second comparison of Anglo, Spanish, and Black children of the unskilled sample was also made. The first analysis comparing 910 Anglo children from the cross-sectional sample with 349 Anglo children from the unskilled families demonstrated significant differences (p smaller than 0.05) for 39 of the 105 items. Below 20 months of age, children of the unskilled sample were more advanced, whereas after 20 months of age, the children of the cross-sectional sample were advanced in all test sectors except for items in the personal-social sector. Comparisons of Anglo, Black, and Spanish surname children from the unskilled families showed fewer differences in rates of development.", "contents": "Development of preschool-aged children of different social and ethnic groups: implications for developmental screening. The developmental status of 1,180 children representing a cross section of Denver's ethnic and parental occupational groups was compared with that of 1,055 children (349 Anglo, 354 Spanish surname, and 352 Black) whose parents were unskilled workers. The children varied in age from 2 weeks to 6.4 years and were evaluated with the Denver developmental screening test. Comparisons were made in developmental achievements as reflected by individual item differences for Anglo children from unskilled families with Anglos from cross-sectional families. A second comparison of Anglo, Spanish, and Black children of the unskilled sample was also made. The first analysis comparing 910 Anglo children from the cross-sectional sample with 349 Anglo children from the unskilled families demonstrated significant differences (p smaller than 0.05) for 39 of the 105 items. Below 20 months of age, children of the unskilled sample were more advanced, whereas after 20 months of age, the children of the cross-sectional sample were advanced in all test sectors except for items in the personal-social sector. Comparisons of Anglo, Black, and Spanish surname children from the unskilled families showed fewer differences in rates of development."}
{"id": "PMID:1097615", "title": "Inadequacies in the pharmacologic management of ambulatory children.", "content": "The compliance with treatment of acute otitis media in 300 pediatric outpatients was evaluated. Complete compliance in taking prescribed antibiotics was noted in 7.3% of the patients. Pharmacists dispensed less than prescribed amounts of antibiotics to 15% of patients. Bottles were incorrectly labeled 3% of the time. Volumes of 130 \"teaspoons\" examined varied from 2 to 9 ml. Parental understanding of the illness and of the effects of medication was inadequate and erroneous in many instances. Parents gave fewer than the prescribed number of doses in 36% of cases, and therapy was discontinued early in 37%. Recommendations for improving the quality of therapy for ambulatory pediatric patients are outlined.", "contents": "Inadequacies in the pharmacologic management of ambulatory children. The compliance with treatment of acute otitis media in 300 pediatric outpatients was evaluated. Complete compliance in taking prescribed antibiotics was noted in 7.3% of the patients. Pharmacists dispensed less than prescribed amounts of antibiotics to 15% of patients. Bottles were incorrectly labeled 3% of the time. Volumes of 130 \"teaspoons\" examined varied from 2 to 9 ml. Parental understanding of the illness and of the effects of medication was inadequate and erroneous in many instances. Parents gave fewer than the prescribed number of doses in 36% of cases, and therapy was discontinued early in 37%. Recommendations for improving the quality of therapy for ambulatory pediatric patients are outlined."}
{"id": "PMID:1097616", "title": "A reappraisal of the value of the IgM fluorescent treponemal antibody absorption test in the diagnosis of congenital syphilis.", "content": "To re-evaluate the usefulness of the IgM fluorescent treponemal antibody absorption test in the diagnosis of congenital syphilis, three groups of infants were investigated. Group 1 infants showed that the test was always negative in normal neonates born to seronegative mothers. From Group 2 infants we determined the standardization of the test using commercially prepared conjugate to allow the maximum exclusion of false positive and negative results. Studies on Group 3 neonates confirmed that the standardized test was extremely successful in determining whether or not an asymptomatic infant was infected with Treponema pallidum. Finally it was shown that the standardization of the test varies with different commercially prepared conjugates. From the investigation it was concluded that the IgM FTA test, if suitably standardized, was a highly successful method for diagnosing congenital syphilis, especially in asymptomatic neonates.", "contents": "A reappraisal of the value of the IgM fluorescent treponemal antibody absorption test in the diagnosis of congenital syphilis. To re-evaluate the usefulness of the IgM fluorescent treponemal antibody absorption test in the diagnosis of congenital syphilis, three groups of infants were investigated. Group 1 infants showed that the test was always negative in normal neonates born to seronegative mothers. From Group 2 infants we determined the standardization of the test using commercially prepared conjugate to allow the maximum exclusion of false positive and negative results. Studies on Group 3 neonates confirmed that the standardized test was extremely successful in determining whether or not an asymptomatic infant was infected with Treponema pallidum. Finally it was shown that the standardization of the test varies with different commercially prepared conjugates. From the investigation it was concluded that the IgM FTA test, if suitably standardized, was a highly successful method for diagnosing congenital syphilis, especially in asymptomatic neonates."}
{"id": "PMID:1097617", "title": "Congential dyserythropoietic anemia--type IV.", "content": "Congenital dyserythropoietic anemia is characterized by ineffective erythropoiesis and increased numbers of multinucleated red cell precursors in the marrow. This syndrome has been subclassified on the basis of morphologic differences in the red cell precursors. Type I is characterized by megaloblastoid erythropoiesis and macrocytosis; Type II, by normoblastic multinuclearity and normocytosis; and Type III, by frequent giant multinucleated erythroblasts and macrocytes. Type II is further distinguished from the other types by serologic and ultrastructural abnormalities. The patient presented in this report does not fit any of the above categories; her red cells are similar to Type II congenital dyserythropoietic anemia cells, but no characteristic ultrastructural or serologic abnormalities are present. It is suggested that this patient may represent an additional variant of congenital dyserythropoietic anemia, Type IV.", "contents": "Congential dyserythropoietic anemia--type IV. Congenital dyserythropoietic anemia is characterized by ineffective erythropoiesis and increased numbers of multinucleated red cell precursors in the marrow. This syndrome has been subclassified on the basis of morphologic differences in the red cell precursors. Type I is characterized by megaloblastoid erythropoiesis and macrocytosis; Type II, by normoblastic multinuclearity and normocytosis; and Type III, by frequent giant multinucleated erythroblasts and macrocytes. Type II is further distinguished from the other types by serologic and ultrastructural abnormalities. The patient presented in this report does not fit any of the above categories; her red cells are similar to Type II congenital dyserythropoietic anemia cells, but no characteristic ultrastructural or serologic abnormalities are present. It is suggested that this patient may represent an additional variant of congenital dyserythropoietic anemia, Type IV."}
{"id": "PMID:1097619", "title": "The early use of continuous positive airway pressure in the treatment of idiopathic respiratory distress syndrome.", "content": "Infants with IRDS were treated with CPAP early (0.40 FIO2; WITH PaO2 less than 60 mm Hg) or late (0.70 FIO2; with PaO2 less than 60 mm Hg). There was no difference in survival, duration of CPAP therapy, total time of oxygen administration, or complications. The early treated infants needed a lower FIO2 (maximum 0.55) and had a less severe clinical course. The late treated infants were subjected to 0.70 or more FIO2 for an average of 24 hours and were in greater than 0.40 FIO2 significantly longer than those given CPAP early. Infants who weighed less than 1,500 gm and had severe disease did not do well regardless of when CPAP was applied.", "contents": "The early use of continuous positive airway pressure in the treatment of idiopathic respiratory distress syndrome. Infants with IRDS were treated with CPAP early (0.40 FIO2; WITH PaO2 less than 60 mm Hg) or late (0.70 FIO2; with PaO2 less than 60 mm Hg). There was no difference in survival, duration of CPAP therapy, total time of oxygen administration, or complications. The early treated infants needed a lower FIO2 (maximum 0.55) and had a less severe clinical course. The late treated infants were subjected to 0.70 or more FIO2 for an average of 24 hours and were in greater than 0.40 FIO2 significantly longer than those given CPAP early. Infants who weighed less than 1,500 gm and had severe disease did not do well regardless of when CPAP was applied."}
{"id": "PMID:1097621", "title": "Energy metabolism and hormonal profile in children with edematous protein-calorie malnutrition.", "content": "Modifications in energy metabolism and endocrine homeostasis (plasma insulin and growth hormone values, glucose and free fatty acid levels, serum thyroxine and TSH, free thyroxine index, and urinary catecholamines) were investigated in eight children with edematous protein-calorie malnutrition. Caloric expenditure was low at admission and correlated linearly with increased caloric intake throughout the study. The hormonal changes at admission were characterized by a negligible insulin response to intravenous arginine or glucose and by markedly elevated growth hormone levels which were neither increased by arginine nor suppressed by intravenous glucose. Serum thyroxine values were low, but free thyroxine index and serum TSH levels were within normal limits. At admission to the study, 24-hour urinary excretion of dopamine and norepinephrine was relatively reduced in relation to the excretion of epinephrine. All these modifications were corrected at time of the recovery study. It is suggested that in edematous protein-calorie malnutrition, insulin acts as the primary regulator of peripheral fuel release and that the high, nonsuppressible growth hormone levels may form part of an important homeostatic mechanism to provide substrates for brain metabolism via lipolysis.", "contents": "Energy metabolism and hormonal profile in children with edematous protein-calorie malnutrition. Modifications in energy metabolism and endocrine homeostasis (plasma insulin and growth hormone values, glucose and free fatty acid levels, serum thyroxine and TSH, free thyroxine index, and urinary catecholamines) were investigated in eight children with edematous protein-calorie malnutrition. Caloric expenditure was low at admission and correlated linearly with increased caloric intake throughout the study. The hormonal changes at admission were characterized by a negligible insulin response to intravenous arginine or glucose and by markedly elevated growth hormone levels which were neither increased by arginine nor suppressed by intravenous glucose. Serum thyroxine values were low, but free thyroxine index and serum TSH levels were within normal limits. At admission to the study, 24-hour urinary excretion of dopamine and norepinephrine was relatively reduced in relation to the excretion of epinephrine. All these modifications were corrected at time of the recovery study. It is suggested that in edematous protein-calorie malnutrition, insulin acts as the primary regulator of peripheral fuel release and that the high, nonsuppressible growth hormone levels may form part of an important homeostatic mechanism to provide substrates for brain metabolism via lipolysis."}
{"id": "PMID:1097628", "title": "Clinical evaluation of a quaternary ammonium-containing mouthrinse.", "content": "This was a double-blind, radomized, crossover study of adult subjects to evaluate the effect of a test mouthrinse (Cepacol) on plaque accumulation. The study was divided into two parts, four weeks each, one in which only a mouthwash was used (part I) and the other in which a mouthwash and toothbrushing were used (part II). 1. The test mouthrinse produced a satistically significant reduction in dental plaque when compared to a placebo rinse. 2. The GI averaged approximately 1.0 throughout the study regardless of which mouthrinse was used. 3. A possible carryover effect of the test mouthrinse was noted. 4. A lower plaque score was seen in 67 to 75% of all patients during the period in which the test mouthrinse was used as compared to the placebo. 5. Ten subjects reported a burning sensation of the tongue with the test mouthrinse. No objective adverse effects were seen.", "contents": "Clinical evaluation of a quaternary ammonium-containing mouthrinse. This was a double-blind, radomized, crossover study of adult subjects to evaluate the effect of a test mouthrinse (Cepacol) on plaque accumulation. The study was divided into two parts, four weeks each, one in which only a mouthwash was used (part I) and the other in which a mouthwash and toothbrushing were used (part II). 1. The test mouthrinse produced a satistically significant reduction in dental plaque when compared to a placebo rinse. 2. The GI averaged approximately 1.0 throughout the study regardless of which mouthrinse was used. 3. A possible carryover effect of the test mouthrinse was noted. 4. A lower plaque score was seen in 67 to 75% of all patients during the period in which the test mouthrinse was used as compared to the placebo. 5. Ten subjects reported a burning sensation of the tongue with the test mouthrinse. No objective adverse effects were seen."}
{"id": "PMID:1097629", "title": "Personality assessment and test interpretation of Mexican Americans: a critique.", "content": "Reviews the personality test assessment of Mexican Americans. Although there is a paucity of available research there are some indications that Mexican Americans differ in response patterning on projective devices. On objective instruments, problems involving fluency in English obscure the findings. Several recommendations are offered for increasing the efficiency of these instruments for use with Mexican American clients.", "contents": "Personality assessment and test interpretation of Mexican Americans: a critique. Reviews the personality test assessment of Mexican Americans. Although there is a paucity of available research there are some indications that Mexican Americans differ in response patterning on projective devices. On objective instruments, problems involving fluency in English obscure the findings. Several recommendations are offered for increasing the efficiency of these instruments for use with Mexican American clients."}
{"id": "PMID:1097630", "title": "Artistic quality of drawings and judges' evaluations of the DAP.", "content": "When art quality varied for a set of figure drawings, judges were unable to differentiate hospitalized schizophrenic females from a matched nonpatient group. Both trained and naive judges erroneously tended to see drawings of low are quality as a patient origin, and drawings of high overall quality as of nonpatient origin. When art quality was held constant judges did slightly 0etter than chance in differentiating the patient vs nonpatient drawings. Psychologists were no more accurate than untrained judges. Results support the position that art quality of drawings influences judges' evaluations, and, in terms of the present investigation, represents a major source of error in drawing interpretation. Even when art quality was controlled, however, judges' rate of success was low, casting doubt on the status validity of the Draw-A-Person test (DAP).", "contents": "Artistic quality of drawings and judges' evaluations of the DAP. When art quality varied for a set of figure drawings, judges were unable to differentiate hospitalized schizophrenic females from a matched nonpatient group. Both trained and naive judges erroneously tended to see drawings of low are quality as a patient origin, and drawings of high overall quality as of nonpatient origin. When art quality was held constant judges did slightly 0etter than chance in differentiating the patient vs nonpatient drawings. Psychologists were no more accurate than untrained judges. Results support the position that art quality of drawings influences judges' evaluations, and, in terms of the present investigation, represents a major source of error in drawing interpretation. Even when art quality was controlled, however, judges' rate of success was low, casting doubt on the status validity of the Draw-A-Person test (DAP)."}
{"id": "PMID:1097631", "title": "Female homosexuals' concepts of self, men, and women.", "content": "Twenty-four female homosexuals and 24 female heterosexuals matched for age and education were given the Draw-A-Person Test (DAP) and, on the Gough-Heilbrun Adjective Check List (ACL), were asked to describe themselves, men in general, and women in general. Results indicated that homosexuals, relative to the heterosuxual controls: (a) sexualized their drawings (p smaller than .02) and embellished the female figure more (p smaller than .05); (b) showed no evidence of confusion in gender identity on the DAP or ACL; (c) scored sifnigicantly higher on Autonomy, Change, and Exhibition on the ACL; and (d) scored significantly lower on Abasement, Defensiveness, Deference, Endurance, Order, Personal Adjustment and Self-control on the ACL. It was concluded that the female homosexual may be more independent, changeable, and sexually preoccupied and less well adjusted than her heterosexual counterpart.", "contents": "Female homosexuals' concepts of self, men, and women. Twenty-four female homosexuals and 24 female heterosexuals matched for age and education were given the Draw-A-Person Test (DAP) and, on the Gough-Heilbrun Adjective Check List (ACL), were asked to describe themselves, men in general, and women in general. Results indicated that homosexuals, relative to the heterosuxual controls: (a) sexualized their drawings (p smaller than .02) and embellished the female figure more (p smaller than .05); (b) showed no evidence of confusion in gender identity on the DAP or ACL; (c) scored sifnigicantly higher on Autonomy, Change, and Exhibition on the ACL; and (d) scored significantly lower on Abasement, Defensiveness, Deference, Endurance, Order, Personal Adjustment and Self-control on the ACL. It was concluded that the female homosexual may be more independent, changeable, and sexually preoccupied and less well adjusted than her heterosexual counterpart."}
{"id": "PMID:1097632", "title": "Gas chromatographic determination and pharmacokinetics of 4-hydroxybutrate in dog and mouse.", "content": "A rapid and reproducible method was developed to extract 4-hydroxybutyrate from plasma as 4-butyrolactone for subsequent gas chromatographic (GLC) assay. The drug, an intravenous anesthetic and oral hypnotic in man, was infused into four dogs and the plasma concentration was determined by 14C-isotope dilution and GLC. Pharmacokinetic parameters for distribution and elimination were calculated. A capacity-limited process appears to be involved in the elimination fo 4-hydroxybutyrate in the dog. Macroautoradiogrpahy recieved the distribution pattern in normal and pregnant adult mice.", "contents": "Gas chromatographic determination and pharmacokinetics of 4-hydroxybutrate in dog and mouse. A rapid and reproducible method was developed to extract 4-hydroxybutyrate from plasma as 4-butyrolactone for subsequent gas chromatographic (GLC) assay. The drug, an intravenous anesthetic and oral hypnotic in man, was infused into four dogs and the plasma concentration was determined by 14C-isotope dilution and GLC. Pharmacokinetic parameters for distribution and elimination were calculated. A capacity-limited process appears to be involved in the elimination fo 4-hydroxybutyrate in the dog. Macroautoradiogrpahy recieved the distribution pattern in normal and pregnant adult mice."}
{"id": "PMID:1097633", "title": "Synthesis of (4-quinolinoamino)aminoalkyltetrahydronaphthalene derivatives for possible antimalarial activity.", "content": "(4-Quinolinoamino)aminoalkyltetrahydronaphthalene derivatives were synthesized in an attempt to introduce new agents with antimalarial activity.", "contents": "Synthesis of (4-quinolinoamino)aminoalkyltetrahydronaphthalene derivatives for possible antimalarial activity. (4-Quinolinoamino)aminoalkyltetrahydronaphthalene derivatives were synthesized in an attempt to introduce new agents with antimalarial activity."}
{"id": "PMID:1097634", "title": "Synthesis and biological properties of alkyl esters of polyene antibiotics.", "content": "Several new alkyl esters of polyene antibiotics were prepared by an improved general procedure, and their toxicity and microbiological activity were tested. Some of these alkyl esters were more active than the nonesterified polyenes against Candida albicans but were less effective than the known methyl esters. Their toxicity was much less than that of the parent compounds.", "contents": "Synthesis and biological properties of alkyl esters of polyene antibiotics. Several new alkyl esters of polyene antibiotics were prepared by an improved general procedure, and their toxicity and microbiological activity were tested. Some of these alkyl esters were more active than the nonesterified polyenes against Candida albicans but were less effective than the known methyl esters. Their toxicity was much less than that of the parent compounds."}
{"id": "PMID:1097635", "title": "Genetic effects of providone-iodine.", "content": "Povidone-iodine is capable of specifically altering the DNA of living cells. This alteration may result in the induction of mutations of the base-substitution type. Because of the known relationship between mutagenic potential and the ability to induce cancer in animals, the present findings raise serious questions concerning the safety of this topical disinfectant.", "contents": "Genetic effects of providone-iodine. Povidone-iodine is capable of specifically altering the DNA of living cells. This alteration may result in the induction of mutations of the base-substitution type. Because of the known relationship between mutagenic potential and the ability to induce cancer in animals, the present findings raise serious questions concerning the safety of this topical disinfectant."}
{"id": "PMID:1097636", "title": "Absorption, distribution, and metabolic fate of 7-chloro-3,3a-dihydro-2-methyl-2H,9H-isoxazolo-(3,2-b)(1,3)-benzoxazin-9-one in rats, dogs, and humans.", "content": "The absorption and metabolic fate of 7-chloro-3,3a-dihydro-2-methyl-2H,9H-isoxazolo-(3,2-b)(1,3)-benzoxazin-9-one (I) was studied in rats, dogs, and humans. Orally administered I was readily absorbed by all species. In the rat, orally administered I was converted to its metabolite, 5-chlorosalicylic acid, by the intestinal wall. The half-lives of blood radioactivity, after the oral administration of I-9-14C, were about 18 and 12 hr in the rat and beagle hound, respectively. In human subjects, no intact I was detected in the bloodstream; however, the clearance of the metabolite, 5-chlorosalicylic acid, had a half-life of about 33 hr. Cleavage of the oxazine ring of I generated 5-chlorosalicylic acid, which was excreted both in the free form and conjugated with glycine and glucuronic acid. The isoxazole moiety was converted to beta-hydroxybutyric acid and its metabolites carbon dioxide and fumaric, citric, alpha-ketoglutaric, succinic, and malic acids. Binding of I to plasma proteins was extensive but was less than that of 5-chlorosalicylic acid.", "contents": "Absorption, distribution, and metabolic fate of 7-chloro-3,3a-dihydro-2-methyl-2H,9H-isoxazolo-(3,2-b)(1,3)-benzoxazin-9-one in rats, dogs, and humans. The absorption and metabolic fate of 7-chloro-3,3a-dihydro-2-methyl-2H,9H-isoxazolo-(3,2-b)(1,3)-benzoxazin-9-one (I) was studied in rats, dogs, and humans. Orally administered I was readily absorbed by all species. In the rat, orally administered I was converted to its metabolite, 5-chlorosalicylic acid, by the intestinal wall. The half-lives of blood radioactivity, after the oral administration of I-9-14C, were about 18 and 12 hr in the rat and beagle hound, respectively. In human subjects, no intact I was detected in the bloodstream; however, the clearance of the metabolite, 5-chlorosalicylic acid, had a half-life of about 33 hr. Cleavage of the oxazine ring of I generated 5-chlorosalicylic acid, which was excreted both in the free form and conjugated with glycine and glucuronic acid. The isoxazole moiety was converted to beta-hydroxybutyric acid and its metabolites carbon dioxide and fumaric, citric, alpha-ketoglutaric, succinic, and malic acids. Binding of I to plasma proteins was extensive but was less than that of 5-chlorosalicylic acid."}
{"id": "PMID:1097637", "title": "The relationship between nitro group reduction and the intestinal microflora.", "content": "The capacity of rats to reduce a 25-mg dose of p-nitrobenzoic acid (PNBA) was measured by quantifying the amount of this compound recovered in the urine as p-aminobenzoic acid (PABA) and its conjugates. It was found that germfree rats converted approximately 1% of PNBA to PABA; in conventional rats the conversion was approximately 25%. Various bacteria isolated from the rat cecum were selectively associated with germfree rats and it was demonstrated that these bacteria colonized their gastrointestinal tracts. In assoication with Lactobacillus plantarum, the conversion of PNBA to PABA increased to 3.9%. When these rats were further associated with Clostridium sp. and Streptoccocus faecalis, the conversion increased to approximately 12%. A general correlation was found between the capacity of constituents of the microflora to reduce PNBA in vitro and when associated with the germfree rat. Cecectomy, which removes a substantial portion of the microflora of the rat, decreases the capacity of the conventional rat to reduce PNBA. Similar experiments with p-nitrobenzenesulfonamide indicate that this compound is also largely reduced by the flora. Evidence that the reduction of the nitro group in these compounds is carried out by the intestinal microflora explains previous observations in which the reduction of these compounds in rats did not correlate with the activity of liver enzymes putative for these reactions.", "contents": "The relationship between nitro group reduction and the intestinal microflora. The capacity of rats to reduce a 25-mg dose of p-nitrobenzoic acid (PNBA) was measured by quantifying the amount of this compound recovered in the urine as p-aminobenzoic acid (PABA) and its conjugates. It was found that germfree rats converted approximately 1% of PNBA to PABA; in conventional rats the conversion was approximately 25%. Various bacteria isolated from the rat cecum were selectively associated with germfree rats and it was demonstrated that these bacteria colonized their gastrointestinal tracts. In assoication with Lactobacillus plantarum, the conversion of PNBA to PABA increased to 3.9%. When these rats were further associated with Clostridium sp. and Streptoccocus faecalis, the conversion increased to approximately 12%. A general correlation was found between the capacity of constituents of the microflora to reduce PNBA in vitro and when associated with the germfree rat. Cecectomy, which removes a substantial portion of the microflora of the rat, decreases the capacity of the conventional rat to reduce PNBA. Similar experiments with p-nitrobenzenesulfonamide indicate that this compound is also largely reduced by the flora. Evidence that the reduction of the nitro group in these compounds is carried out by the intestinal microflora explains previous observations in which the reduction of these compounds in rats did not correlate with the activity of liver enzymes putative for these reactions."}
{"id": "PMID:1097638", "title": "Intestinal smooth muscle contraction and the effects of cadmium and A23187.", "content": "Pretreatment of the guinea-pig ileal longitudinal muscle preparation with cadmium (Cd++) in the concentration range 5 to 1000 muM resulted in an inhibition of the phasic and tonic contractions induced by both high potassium and the muscarinic agonist, cis-2-methyl-4-dimethylaminomethyl-1,3-dioxolane methiodide. The K+ contracture was significantly more sensitive to the inhibitory action of Cd++ in the concentration range 5 to 100 muM, but not at concentrations of Cd++ above 100 muM. Low concentrations of Cd++, 0.1 to 0.01 muM, were found to increase by an atropine-sensitive mechanism, the spontaneous activity of approximately 50% of the preparations tested. It was also shown that the ionophore A23187 produced a concentration-dependent contraction in the guinea-pig ileal longitudinal muscle that was dependent upon the presence of extracellular calcium. When 1.8 mM Cd++ was added to a tissue that had been incubated in Ca++-free media for 60 minutes and pretreated with 5 muM A23187 for 15 minutes, a slow contractile response was recorded. Similar, but significantly smaller, contractions were also recorded when 1.8 mM Ca++, Mn++, Sr++, Pb++ and Ba++, but not when Co++, Ni++, Hg++, UO2++, and Tm++ and Th++++ were administered. It was concluded that the contractile action of these cations was dependent on ionic radius, hydration energy, valency and, perhaps, coordination number, and was due to the intracellular entry of the cation where it resulted in the release of bound Ca++ and/or directly activated the smooth muscle troponin system.", "contents": "Intestinal smooth muscle contraction and the effects of cadmium and A23187. Pretreatment of the guinea-pig ileal longitudinal muscle preparation with cadmium (Cd++) in the concentration range 5 to 1000 muM resulted in an inhibition of the phasic and tonic contractions induced by both high potassium and the muscarinic agonist, cis-2-methyl-4-dimethylaminomethyl-1,3-dioxolane methiodide. The K+ contracture was significantly more sensitive to the inhibitory action of Cd++ in the concentration range 5 to 100 muM, but not at concentrations of Cd++ above 100 muM. Low concentrations of Cd++, 0.1 to 0.01 muM, were found to increase by an atropine-sensitive mechanism, the spontaneous activity of approximately 50% of the preparations tested. It was also shown that the ionophore A23187 produced a concentration-dependent contraction in the guinea-pig ileal longitudinal muscle that was dependent upon the presence of extracellular calcium. When 1.8 mM Cd++ was added to a tissue that had been incubated in Ca++-free media for 60 minutes and pretreated with 5 muM A23187 for 15 minutes, a slow contractile response was recorded. Similar, but significantly smaller, contractions were also recorded when 1.8 mM Ca++, Mn++, Sr++, Pb++ and Ba++, but not when Co++, Ni++, Hg++, UO2++, and Tm++ and Th++++ were administered. It was concluded that the contractile action of these cations was dependent on ionic radius, hydration energy, valency and, perhaps, coordination number, and was due to the intracellular entry of the cation where it resulted in the release of bound Ca++ and/or directly activated the smooth muscle troponin system."}
{"id": "PMID:1097639", "title": "Ionic mechanism of the efferent olivo-cochlear inhibition studied by cochlear perfusion in the cat.", "content": "1. A method for perfusing the scala tympani of the cat's cochlea from basal turn to apex is described. The perfusion with modified Krebs solution did not interfere with the recording of cochlear microphonic (CM) and neural responses to sound, nor with the efferent inhibition elicited by stereotaxic stimulation of the crossed olivo-cochlear bundle (COCB) in the medulla. 2. Cochlear perfusion with solutions in which most of the chloride was replaced by large anions (sulphate or gluconate) decreased or eliminated auditory nerve or the ventral cochlear nucleus. These effects were reversible. They were only observed if the rate of perfusion (2-20 mul/min) was adequate to reduce the chloride concentration in perilymph below about 80 mM, this being estimated, in different perfusion of the same cochlea, by a chloride-selective electrode. 3. The COCB-induced negative shift of the endocochlear potential recorded with a glass micro-electrode inserted into the scala media was abolished by I.V. strychnine o.2 mg/kg. It was decreased when the perilymph chloride was reduced to 50-70 mM and could be abolished when the perilymph chloride dropped to about 5 mM. 4. The COCB-induced potentiation of the cochlear microphonic potential was also reduced by chloride substitution but the pattern of this effect differed from that of neural inhibition. 5. Similar cochlear perfusions with a solution in which the small diameter bromide anion was substituted for chloride did not affect the COCB-efferent effects. 6. The data indicate that the inhibitory transmitter released by COCB terminals elicits an increased conductance to small anions (normally to chloride) in the membrane of the auditory dendrite and of the outer hair cell. The significance of the COCB-induced negative shift of endocochlear potential and of the potentiation of CM is discussed, as well as the pre- and post-synaptic mechanisms involved in the efferent gating exerted on the auditory input. The latter would seem to involve primarily a post-synaptic mechanism at efferent axo-dendritic synapses.", "contents": "Ionic mechanism of the efferent olivo-cochlear inhibition studied by cochlear perfusion in the cat. 1. A method for perfusing the scala tympani of the cat's cochlea from basal turn to apex is described. The perfusion with modified Krebs solution did not interfere with the recording of cochlear microphonic (CM) and neural responses to sound, nor with the efferent inhibition elicited by stereotaxic stimulation of the crossed olivo-cochlear bundle (COCB) in the medulla. 2. Cochlear perfusion with solutions in which most of the chloride was replaced by large anions (sulphate or gluconate) decreased or eliminated auditory nerve or the ventral cochlear nucleus. These effects were reversible. They were only observed if the rate of perfusion (2-20 mul/min) was adequate to reduce the chloride concentration in perilymph below about 80 mM, this being estimated, in different perfusion of the same cochlea, by a chloride-selective electrode. 3. The COCB-induced negative shift of the endocochlear potential recorded with a glass micro-electrode inserted into the scala media was abolished by I.V. strychnine o.2 mg/kg. It was decreased when the perilymph chloride was reduced to 50-70 mM and could be abolished when the perilymph chloride dropped to about 5 mM. 4. The COCB-induced potentiation of the cochlear microphonic potential was also reduced by chloride substitution but the pattern of this effect differed from that of neural inhibition. 5. Similar cochlear perfusions with a solution in which the small diameter bromide anion was substituted for chloride did not affect the COCB-efferent effects. 6. The data indicate that the inhibitory transmitter released by COCB terminals elicits an increased conductance to small anions (normally to chloride) in the membrane of the auditory dendrite and of the outer hair cell. The significance of the COCB-induced negative shift of endocochlear potential and of the potentiation of CM is discussed, as well as the pre- and post-synaptic mechanisms involved in the efferent gating exerted on the auditory input. The latter would seem to involve primarily a post-synaptic mechanism at efferent axo-dendritic synapses."}
{"id": "PMID:1097640", "title": "Gastric, antral and fundic pouch secretion in sheep.", "content": "1. Fundic secretion of HCl and pepsin was studied in sheep with both fundic and antral pouches. The antral pouches were of the entire pyloric region. Continuity of the alimentary tract was restored by an abomasoduodenal anastomosis. 2. Secretion from fundic pouches was continuous. It was reduced in volume and acidity, was pepsin output, by resection of the antral pouches. 3. Teasing with food, feeding and injection of pentagastrin stimulated fundic acid and pepsin secretion in animals with antral pouches before and after antrectomy. 4. Pouches prepared from the entire pyloric region showed continuous secretion with variations not related to feeding or fasting. Antral secretion was increased after s.c. injections of histamine, carbachol and pentagastrin. 5. It is concluded that although the pyloric antrum contributes to the magnitude of the secretory response of an abomasal fundic pouch, the direction of the secretory response is similar whether or not the pyloric antrum is present as a separated pouch, or is removed.", "contents": "Gastric, antral and fundic pouch secretion in sheep. 1. Fundic secretion of HCl and pepsin was studied in sheep with both fundic and antral pouches. The antral pouches were of the entire pyloric region. Continuity of the alimentary tract was restored by an abomasoduodenal anastomosis. 2. Secretion from fundic pouches was continuous. It was reduced in volume and acidity, was pepsin output, by resection of the antral pouches. 3. Teasing with food, feeding and injection of pentagastrin stimulated fundic acid and pepsin secretion in animals with antral pouches before and after antrectomy. 4. Pouches prepared from the entire pyloric region showed continuous secretion with variations not related to feeding or fasting. Antral secretion was increased after s.c. injections of histamine, carbachol and pentagastrin. 5. It is concluded that although the pyloric antrum contributes to the magnitude of the secretory response of an abomasal fundic pouch, the direction of the secretory response is similar whether or not the pyloric antrum is present as a separated pouch, or is removed."}
{"id": "PMID:1097648", "title": "A denture tray technique for remake dentures.", "content": "A technique is described to fabricate a replacement denture for your patient. It reduces patient and laboratory time. The technique does not compromise the patient's existing denture, the impression, jaw relation records, or esthetics. It provides superior trays and effective occlusion rims, thus simplifying recording techniques. A denture duplicating flask may be substituted for the two plastic impression trays to duplicate the denture. The duplicating flask may improve realignment of the upper and lower molds, but additional irreversible hydrocolloid is required to fill the mold.", "contents": "A denture tray technique for remake dentures. A technique is described to fabricate a replacement denture for your patient. It reduces patient and laboratory time. The technique does not compromise the patient's existing denture, the impression, jaw relation records, or esthetics. It provides superior trays and effective occlusion rims, thus simplifying recording techniques. A denture duplicating flask may be substituted for the two plastic impression trays to duplicate the denture. The duplicating flask may improve realignment of the upper and lower molds, but additional irreversible hydrocolloid is required to fill the mold."}
{"id": "PMID:1097650", "title": "Postinsertion changes in complete dentures.", "content": "The influence of denture changes, occurring during a period of use ranging from one to 15 years, was studied in a group of 35 complete denture subjects. Clinical and electromyographic data were collected. N0 correlation was found between denture serviceability on the one hand and masticatory performance and muscular coordination during chewing of test food on the other. However, a significant correlation was found between a subject's satisfaction and his masticatory performance, as well as his muscular coordination during chewing of test food. It appears that, as soon as the time-linked and insidious imperfections of complete dentures are compensated for by feedback-controlled neuromuscular adaptation, the subject's adjusted pattern of function does not affect his performance and comfort and consequently his satisfaction. Despite the obvious differences among the respective subjects, the number of masticatory strokes in the same individual was rather constant. Obviously, a subject's satisfaction may easily camouflage lack of denture fit, including its potential concomitant pathologic sequelae. Therefore, periodic examinations and adequate corrections are essential to successful complete denture treatment.", "contents": "Postinsertion changes in complete dentures. The influence of denture changes, occurring during a period of use ranging from one to 15 years, was studied in a group of 35 complete denture subjects. Clinical and electromyographic data were collected. N0 correlation was found between denture serviceability on the one hand and masticatory performance and muscular coordination during chewing of test food on the other. However, a significant correlation was found between a subject's satisfaction and his masticatory performance, as well as his muscular coordination during chewing of test food. It appears that, as soon as the time-linked and insidious imperfections of complete dentures are compensated for by feedback-controlled neuromuscular adaptation, the subject's adjusted pattern of function does not affect his performance and comfort and consequently his satisfaction. Despite the obvious differences among the respective subjects, the number of masticatory strokes in the same individual was rather constant. Obviously, a subject's satisfaction may easily camouflage lack of denture fit, including its potential concomitant pathologic sequelae. Therefore, periodic examinations and adequate corrections are essential to successful complete denture treatment."}
{"id": "PMID:1097651", "title": "The use of natural teeth in overlay dentures.", "content": "A method has been described for the construction of tooth-supported dentures where the natural tooth was utilized and the acrylic resin for the denture base processed directly to the prepared cast. Based on the 112 dentures that were made, this technique is economical, provides support and stabilization, and has full patient acceptance.", "contents": "The use of natural teeth in overlay dentures. A method has been described for the construction of tooth-supported dentures where the natural tooth was utilized and the acrylic resin for the denture base processed directly to the prepared cast. Based on the 112 dentures that were made, this technique is economical, provides support and stabilization, and has full patient acceptance."}
{"id": "PMID:1097652", "title": "Conditioning dentures for problem patients.", "content": "We have had considerable success with difficult patients through the use of conditioning dentures. Patients with severe gagging problems have been able to gradually accustom themselves to the presence of dentures in the privacy of their homes and at their own rate of progression. Patients without previous denture experience are given a chance to adjust to problems of speech, salivation, and taste prior to receiving their new dentures. We believe that conditioning dentures should be a part of the treatment sequence for all patients being prepared for their first dentures. Many problems are faced by the new denture patient, and anything the dentist can do to prepare him for this difficult time can increase the chances for success.", "contents": "Conditioning dentures for problem patients. We have had considerable success with difficult patients through the use of conditioning dentures. Patients with severe gagging problems have been able to gradually accustom themselves to the presence of dentures in the privacy of their homes and at their own rate of progression. Patients without previous denture experience are given a chance to adjust to problems of speech, salivation, and taste prior to receiving their new dentures. We believe that conditioning dentures should be a part of the treatment sequence for all patients being prepared for their first dentures. Many problems are faced by the new denture patient, and anything the dentist can do to prepare him for this difficult time can increase the chances for success."}
{"id": "PMID:1097653", "title": "Stressbreakers and the removable partial denture.", "content": "A laboratory study was designed to determine the effect of \"\"deactivation'' of stressbreakers on abutment tooth movement and ridge displacement. The Ticonium hidden-lock and the pin-Dalbo precision stressbreakers were tested. Results showed that ridge displacement was significantly decreased when the Dalbo was made rigid, and tooth movement was significantly decreased (during bilateral loading) when the hidden lock was made rigid.", "contents": "Stressbreakers and the removable partial denture. A laboratory study was designed to determine the effect of \"\"deactivation'' of stressbreakers on abutment tooth movement and ridge displacement. The Ticonium hidden-lock and the pin-Dalbo precision stressbreakers were tested. Results showed that ridge displacement was significantly decreased when the Dalbo was made rigid, and tooth movement was significantly decreased (during bilateral loading) when the hidden lock was made rigid."}
{"id": "PMID:1097654", "title": "Preparing guide planes for removable partial dentures.", "content": "The freehand technique for preparing parallel guide planes offers several advantages. There is a direct transfer of the index from the surveyor to the mouth. The procedure adds little time to routine removable partial denture construction. The method is effective. The only disadvantage of the method is the inability of the dentist to clinically establish perfect parallelism. An occlusal divergence of 3 to 4 degrees from absolute parallelism may result. Care in making the index and increased skill will reduce inaccuracies.", "contents": "Preparing guide planes for removable partial dentures. The freehand technique for preparing parallel guide planes offers several advantages. There is a direct transfer of the index from the surveyor to the mouth. The procedure adds little time to routine removable partial denture construction. The method is effective. The only disadvantage of the method is the inability of the dentist to clinically establish perfect parallelism. An occlusal divergence of 3 to 4 degrees from absolute parallelism may result. Care in making the index and increased skill will reduce inaccuracies."}
{"id": "PMID:1097655", "title": "The paradoxes of fixed prosthodontics.", "content": "(1) The replacement of a missing tooth or teeth by a fixed prosthesis as a preventive device is a paradox. (2) When a missing tooth is replaced by a fixed prosthesis, iatrogenic problems may occur. (3) The problems caused by the fabrication of a fixed partial denture must be recognized and controlled by both the patient and dentist. (4) Concerning the prognosis of any particular fixed prosthesis, we can only predict, from experience, the longevity of the replacement. The acutal life of the replacement is dependent upon the proficiency used in the maintenance procedures and the ability of the dentist to reduce or minimize the iatrogenic factors inherent in any fixed bridge.", "contents": "The paradoxes of fixed prosthodontics. (1) The replacement of a missing tooth or teeth by a fixed prosthesis as a preventive device is a paradox. (2) When a missing tooth is replaced by a fixed prosthesis, iatrogenic problems may occur. (3) The problems caused by the fabrication of a fixed partial denture must be recognized and controlled by both the patient and dentist. (4) Concerning the prognosis of any particular fixed prosthesis, we can only predict, from experience, the longevity of the replacement. The acutal life of the replacement is dependent upon the proficiency used in the maintenance procedures and the ability of the dentist to reduce or minimize the iatrogenic factors inherent in any fixed bridge."}
{"id": "PMID:1097656", "title": "A study of temperature changes during removal of amalgam restorations.", "content": "Because tooth preparation is only one of several potent causes of trauma to the pulp during clinical procedures, the objective should be to increase the chance of pulp survival by reducing this trauma wheneve- possible. In case of tooth preparation, this study shows that there is far less potential for pulpal damage when water is used as a coolant.", "contents": "A study of temperature changes during removal of amalgam restorations. Because tooth preparation is only one of several potent causes of trauma to the pulp during clinical procedures, the objective should be to increase the chance of pulp survival by reducing this trauma wheneve- possible. In case of tooth preparation, this study shows that there is far less potential for pulpal damage when water is used as a coolant."}
{"id": "PMID:1097657", "title": "Anterior condylar displacement: its diagnosis and treatment.", "content": "A deflective slide in centric relation to centric occlusion does not necessarily mean anterior condylar displacement. Its diagnosis and treatment depend on the correlation of three factors: the direction and magnitude of the mandibular slide from centric relation to centric occlusion, the change in vertical dimension of occlusion during the slide, and the position of the condyles in the fossae when the teeth are in the maximum occlusion (centric occlusion). When the change in vertical dimension almost equals to amount of slide from the deflective contact in centric relation to maximum intercuspation, very little anterior condylar displacement would be expected. Conversely, with proportionately little change in vertical dimension, more anterior condylar translation is required for a given degree of anterior slide. Examples of each type of anterior slide were related to the TMJ radiographs of the condylar position. If the direction and magnitude of the deflective occlusal contact can be correlated with the TMJ radiographs, the centric relation is \"\"functional,'' and the clinically retruded mandibular position should be used. When this correlation does not exist, the centric relation is \"\"dysfunctional'' and the terminal hinge position (retruded mandibular position) should not be used for restorative or corrective procedures. Examples of anterior condylar displacement were given, including deviation, with a comparison of \"\"before'' and \"\"after'' TMJ radiographs.", "contents": "Anterior condylar displacement: its diagnosis and treatment. A deflective slide in centric relation to centric occlusion does not necessarily mean anterior condylar displacement. Its diagnosis and treatment depend on the correlation of three factors: the direction and magnitude of the mandibular slide from centric relation to centric occlusion, the change in vertical dimension of occlusion during the slide, and the position of the condyles in the fossae when the teeth are in the maximum occlusion (centric occlusion). When the change in vertical dimension almost equals to amount of slide from the deflective contact in centric relation to maximum intercuspation, very little anterior condylar displacement would be expected. Conversely, with proportionately little change in vertical dimension, more anterior condylar translation is required for a given degree of anterior slide. Examples of each type of anterior slide were related to the TMJ radiographs of the condylar position. If the direction and magnitude of the deflective occlusal contact can be correlated with the TMJ radiographs, the centric relation is \"\"functional,'' and the clinically retruded mandibular position should be used. When this correlation does not exist, the centric relation is \"\"dysfunctional'' and the terminal hinge position (retruded mandibular position) should not be used for restorative or corrective procedures. Examples of anterior condylar displacement were given, including deviation, with a comparison of \"\"before'' and \"\"after'' TMJ radiographs."}
{"id": "PMID:1097658", "title": "Fabrication of intraoral radiotherapy prostheses.", "content": "An accurate, short method of fabricating intraoral radiotherapy prostheses has been described. With it, there is no need of mounting casts on an articulator, wax-up, or flasking. Almost all procedures can be accomplished by the dental technician. Most important, the prostheses can be ready for use a few hours after the impressions are made. The technician can prepare the walls and cylinders beforehand and keep them in stock until needed. Also, it would be advisable for the radiotherapist to be present at the initial fastening of the bases to the cylinders. In this manner, the accuracy of the function of the prosthesis will be assured.", "contents": "Fabrication of intraoral radiotherapy prostheses. An accurate, short method of fabricating intraoral radiotherapy prostheses has been described. With it, there is no need of mounting casts on an articulator, wax-up, or flasking. Almost all procedures can be accomplished by the dental technician. Most important, the prostheses can be ready for use a few hours after the impressions are made. The technician can prepare the walls and cylinders beforehand and keep them in stock until needed. Also, it would be advisable for the radiotherapist to be present at the initial fastening of the bases to the cylinders. In this manner, the accuracy of the function of the prosthesis will be assured."}
{"id": "PMID:1097661", "title": "Effect of the antiphagocytic agent cytochalasin B on macrophage invasion by Leishmania mexicana promastigotes and Trypanosoma cruzi epimastigotes.", "content": "Unstimulated mouse peritoneal exudate cells were cultured on coverslips in Medium 199 containing 10 per cent (v/v) calf serum. Cytochalasin B dissolved in dimethyl sulphoxide (DMSO) and diluted in Medium 199 was added to cultures to give final concentrations of 1, 5 and 10 mug/ml. Equal numbers of Leishmania mexicana promastigotes, Trypanosoma cruzi epimastigotes and sheep red cells were added to 24 hr cultures incubated at 37 C. The macrophage monolayers were fixed and stained at various time intervals. L. mexicana promastigotes sheep red blood cells were found to attach to macrophages in the presence of the drug but did not enter the cells. When the medium containing the cytochalasin was replaced with normal medium phagocytosis of the adherent parasites and red cells followed rapidly. T. cruzi epimastigotes were found inside macrophages in both drug-treated and drug-free cultures although the number found to be intracellular in the latter was significantly greater. This study suggest that L. mexicana promastigotes enter macrophages by being phagocytosed, whereas T. cruzi epimastigotes can actively penetrate these cells.", "contents": "Effect of the antiphagocytic agent cytochalasin B on macrophage invasion by Leishmania mexicana promastigotes and Trypanosoma cruzi epimastigotes. Unstimulated mouse peritoneal exudate cells were cultured on coverslips in Medium 199 containing 10 per cent (v/v) calf serum. Cytochalasin B dissolved in dimethyl sulphoxide (DMSO) and diluted in Medium 199 was added to cultures to give final concentrations of 1, 5 and 10 mug/ml. Equal numbers of Leishmania mexicana promastigotes, Trypanosoma cruzi epimastigotes and sheep red cells were added to 24 hr cultures incubated at 37 C. The macrophage monolayers were fixed and stained at various time intervals. L. mexicana promastigotes sheep red blood cells were found to attach to macrophages in the presence of the drug but did not enter the cells. When the medium containing the cytochalasin was replaced with normal medium phagocytosis of the adherent parasites and red cells followed rapidly. T. cruzi epimastigotes were found inside macrophages in both drug-treated and drug-free cultures although the number found to be intracellular in the latter was significantly greater. This study suggest that L. mexicana promastigotes enter macrophages by being phagocytosed, whereas T. cruzi epimastigotes can actively penetrate these cells."}
{"id": "PMID:1097662", "title": "[Evaluation of the parietal factor interfering in the measurement of cardiac output by external detection with the aid of 99mTc labelled red cells (author's transl)].", "content": "The results of a radioisotopic exploration are not independant of the physical characteristics of the tracer used. Thus in the measurement of cardiac output index by external detection, the substitution of 131I fastened on the human serum albumine by 99mTc fastened on red cells introduces a systematic average error of +20% whose origin is double. Because of the plasma or red cell kind of the tracer, the average error done on the volume is, in 130 measurements, 26% whereas that which results from the difference of photon energy is -6%. Only this last error affects the measurement of the cardiac output and, by that time, it is demonstrated that a direct determination of the cardiac output index by STEWART-HAMILTON'S relation is impossible.", "contents": "[Evaluation of the parietal factor interfering in the measurement of cardiac output by external detection with the aid of 99mTc labelled red cells (author's transl)]. The results of a radioisotopic exploration are not independant of the physical characteristics of the tracer used. Thus in the measurement of cardiac output index by external detection, the substitution of 131I fastened on the human serum albumine by 99mTc fastened on red cells introduces a systematic average error of +20% whose origin is double. Because of the plasma or red cell kind of the tracer, the average error done on the volume is, in 130 measurements, 26% whereas that which results from the difference of photon energy is -6%. Only this last error affects the measurement of the cardiac output and, by that time, it is demonstrated that a direct determination of the cardiac output index by STEWART-HAMILTON'S relation is impossible."}
{"id": "PMID:1097664", "title": "[Radiographic aspects of aseptic osteonecrosis following renal transplantation. Apropos of 65 localizations in 30 patients].", "content": "The authors report 30 cases of aseptic osteonecrosis in 65 different bones, including 37 hips, 13 knees, 5 taluses, 1 cuboid, 8 shoulders, 1 humeral condyle, following renal transplantation. One patient had osteonecrosis in six different bones. In 24 cases, the necrosis occurred before the end of the 18th month following the graftmin 21 cases, radiodiagnosis of the first localisation was made before the end of the first month following the first clinical sign. The usual presenting symptom of osteonecrosis is a clear, sub-chondral crescent. In 2/3rds of cases of osteonecrosis of the femoral neck, there is a rapidly destructive form often well tolerated clinically. In 1/3rd of cases, the course was slow with mainly osteocondensation. The course is particularly destructive when, following failure of the graft, the transplanted patient is submitted to periodic hemodialysis. In 8 cases, the femoral condyles were involved, and in 4, the tibial condylesm the appearance may be that of osteochondritis dissecans, but more often the course resembles the destructive form of aseptic necrosis of the femoral head. Shoulder involvement leads to a radiological picture identical with that of the hips. One transplanted patient out of 5, has a chance to develop osteonecrosis within two years following the graft.", "contents": "[Radiographic aspects of aseptic osteonecrosis following renal transplantation. Apropos of 65 localizations in 30 patients]. The authors report 30 cases of aseptic osteonecrosis in 65 different bones, including 37 hips, 13 knees, 5 taluses, 1 cuboid, 8 shoulders, 1 humeral condyle, following renal transplantation. One patient had osteonecrosis in six different bones. In 24 cases, the necrosis occurred before the end of the 18th month following the graftmin 21 cases, radiodiagnosis of the first localisation was made before the end of the first month following the first clinical sign. The usual presenting symptom of osteonecrosis is a clear, sub-chondral crescent. In 2/3rds of cases of osteonecrosis of the femoral neck, there is a rapidly destructive form often well tolerated clinically. In 1/3rd of cases, the course was slow with mainly osteocondensation. The course is particularly destructive when, following failure of the graft, the transplanted patient is submitted to periodic hemodialysis. In 8 cases, the femoral condyles were involved, and in 4, the tibial condylesm the appearance may be that of osteochondritis dissecans, but more often the course resembles the destructive form of aseptic necrosis of the femoral head. Shoulder involvement leads to a radiological picture identical with that of the hips. One transplanted patient out of 5, has a chance to develop osteonecrosis within two years following the graft."}
{"id": "PMID:1097671", "title": "The macromolecular organization of membranes and its bearing on events leading up to fertilization.", "content": "Current concepts of the macromolecular organization of membrances and of ways in which membrane organization may change during membrane fusion are considered briefly. The properties of the sperm membrane at different phases of its development up to fertilization are then examined in the light of this more general model.", "contents": "The macromolecular organization of membranes and its bearing on events leading up to fertilization. Current concepts of the macromolecular organization of membrances and of ways in which membrane organization may change during membrane fusion are considered briefly. The properties of the sperm membrane at different phases of its development up to fertilization are then examined in the light of this more general model."}
{"id": "PMID:1097673", "title": "A comparison of gas and liquid for hysteroscopy.", "content": "Attempts to inspect the uterine cavity heretofore have been thwarted because of inability to obtain its sustained adequate expansion, concern about introducing infection into the peritoneal cavity through the tube with liquid or gaseous media and the provocation of endometrial bleeding during manipulation. Recent mechanical and optical innovations may give the gynecologist the capability of overcoming these problems. In this presentation the focus was on the comparison of gas and liquid as the distending medium for hysteroscopy.", "contents": "A comparison of gas and liquid for hysteroscopy. Attempts to inspect the uterine cavity heretofore have been thwarted because of inability to obtain its sustained adequate expansion, concern about introducing infection into the peritoneal cavity through the tube with liquid or gaseous media and the provocation of endometrial bleeding during manipulation. Recent mechanical and optical innovations may give the gynecologist the capability of overcoming these problems. In this presentation the focus was on the comparison of gas and liquid as the distending medium for hysteroscopy."}
{"id": "PMID:1097678", "title": "Marrow origin of canine alveolar macrophages.", "content": "Pulmonary macrophages were obtained from a canine radiation chimera 221 days after irradiation. A variant of soluble glutamic oxaloacetic transaminase was utilized to demonstrate that these cells were of donor origin, providing evidence that pulmonary macrophages in the dog are of marrow origin.", "contents": "Marrow origin of canine alveolar macrophages. Pulmonary macrophages were obtained from a canine radiation chimera 221 days after irradiation. A variant of soluble glutamic oxaloacetic transaminase was utilized to demonstrate that these cells were of donor origin, providing evidence that pulmonary macrophages in the dog are of marrow origin."}
{"id": "PMID:1097679", "title": "The influence of antigenic stimulation on the ontegeny of lymphoid aggregates and immunoglobulin-containing cells in mouse bronchial and intestinal mucosa.", "content": "The requirement of Ag for the localization of precursors of IgA-producing cells in mucosal surfaces was tested in a model in which fetal tissues develop in an Ag-free adultA/J mice. At 30 days after transplantation primitive Peyer's patches )gut associated lymphoid tissue) and bronchus associated lymphoid tissue were seen in 1/3 of the animals examined and in all animals examined at 60 days. Immunoglobulin A-containing cells first appeared between 5 and 15 days in transplanted fetal guts. Their numbers increased to reach a plateau at 60 days at which time they approximated that of germ-free animals and were about 1/3 that of 60 day old normal mice. Immunoglobulin G and IgM containing cells were rarely seen, Few Ig-containing cells were present in fetal lungs. Fetal gut lumenal IgA sedimented in sucrose density gradients as if it were larger than serum IgA. Thus, in the absence of Ag, IgA plasma cells are present in mucosal tissues but their numbers are reduced.", "contents": "The influence of antigenic stimulation on the ontegeny of lymphoid aggregates and immunoglobulin-containing cells in mouse bronchial and intestinal mucosa. The requirement of Ag for the localization of precursors of IgA-producing cells in mucosal surfaces was tested in a model in which fetal tissues develop in an Ag-free adultA/J mice. At 30 days after transplantation primitive Peyer's patches )gut associated lymphoid tissue) and bronchus associated lymphoid tissue were seen in 1/3 of the animals examined and in all animals examined at 60 days. Immunoglobulin A-containing cells first appeared between 5 and 15 days in transplanted fetal guts. Their numbers increased to reach a plateau at 60 days at which time they approximated that of germ-free animals and were about 1/3 that of 60 day old normal mice. Immunoglobulin G and IgM containing cells were rarely seen, Few Ig-containing cells were present in fetal lungs. Fetal gut lumenal IgA sedimented in sucrose density gradients as if it were larger than serum IgA. Thus, in the absence of Ag, IgA plasma cells are present in mucosal tissues but their numbers are reduced."}
{"id": "PMID:1097682", "title": "Nuclear antigens and antinuclear antibodies-their role in joint inflammation.", "content": "Nuclear antigens, including native DNA, denatured single-stranded DNA and soluble nucleoprotein are found in synovial effusions from a majority of all types of inflammatory joint disease. On the other hand, antinuclear antibodies are rarely detected in joint fluids, except in rheumatoid arthritis. Evidence that nucleoprotein and DNA immune complexes contribute to rheumatoid joint inflammation is reviewed.", "contents": "Nuclear antigens and antinuclear antibodies-their role in joint inflammation. Nuclear antigens, including native DNA, denatured single-stranded DNA and soluble nucleoprotein are found in synovial effusions from a majority of all types of inflammatory joint disease. On the other hand, antinuclear antibodies are rarely detected in joint fluids, except in rheumatoid arthritis. Evidence that nucleoprotein and DNA immune complexes contribute to rheumatoid joint inflammation is reviewed."}
{"id": "PMID:1097688", "title": "Inhibition of phenylalanyl-tRNA synthetase by aromatic guanidines and amidines.", "content": "Aromatic guanidines and amidines were investigated for their ability to inhibit phenylalanyl-tRNA synthetase from E. coli B. 2-Phenylacetamidine (1), benzylguanidine (2), and N-benzylbenzamidine (3) are competive inhibitors with respect to phenylalanine, binding nearly as well as the substrate, The remainder of the inhibitors was unexpectedly found to be noncompetitive, indication the presence of a secondary binding site on the enzyme. Inhibition by these compounds appears to be specific for phenylalanyl-tRNA synthetase and requires the presence of a phenyl ring as well as the amidine or guanidine moiety.", "contents": "Inhibition of phenylalanyl-tRNA synthetase by aromatic guanidines and amidines. Aromatic guanidines and amidines were investigated for their ability to inhibit phenylalanyl-tRNA synthetase from E. coli B. 2-Phenylacetamidine (1), benzylguanidine (2), and N-benzylbenzamidine (3) are competive inhibitors with respect to phenylalanine, binding nearly as well as the substrate, The remainder of the inhibitors was unexpectedly found to be noncompetitive, indication the presence of a secondary binding site on the enzyme. Inhibition by these compounds appears to be specific for phenylalanyl-tRNA synthetase and requires the presence of a phenyl ring as well as the amidine or guanidine moiety."}
{"id": "PMID:1097689", "title": "Pattern recognitiion and structure-activity relationship studies. Computer-assisted prediction of antitumor activity in structurally diverse drugs in an experimental mouse brain tumor system.", "content": "This paper reports the application of pattern recognition and substructural analysis to the problem of predicting the antineoplastic activity of 24 test compounds in an experimental mouse brain tumor system based on 138 structurally diverse compounds tested in this tumor system. The molecules were represented by three types of substructural fragments, the augmented atom, the heteropath, and the ring fragments. Of the two pattern recognition methods used to predict the activity of the test compounds the nearest neighbor method predicted 83% correctly while the learning machine method predicted 92% correctly. The test structures and the important substructural fragments used in this study are given and the implications of these results are discussed.", "contents": "Pattern recognitiion and structure-activity relationship studies. Computer-assisted prediction of antitumor activity in structurally diverse drugs in an experimental mouse brain tumor system. This paper reports the application of pattern recognition and substructural analysis to the problem of predicting the antineoplastic activity of 24 test compounds in an experimental mouse brain tumor system based on 138 structurally diverse compounds tested in this tumor system. The molecules were represented by three types of substructural fragments, the augmented atom, the heteropath, and the ring fragments. Of the two pattern recognition methods used to predict the activity of the test compounds the nearest neighbor method predicted 83% correctly while the learning machine method predicted 92% correctly. The test structures and the important substructural fragments used in this study are given and the implications of these results are discussed."}
{"id": "PMID:1097690", "title": "Synthesis and biological activity of luteinizing hormone-releasing hormone and related peptides.", "content": "Syntheses of the decapeptide luteinizing hormone-releasing hormone, less thanGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 are described. The basic properties of arginine can provide a simple repetitive isolation procedure for arginine-containing peptides. The biological activities of the decapeptide, of a range of fragments and modified fragments, and of two analogs with alteration in the series at position 4 were measured by in vitro incubation with sheep pituitary slices, measuring the liberated LH by bioassay. None of the compounds of shortened sequence were active, with the exception of less thanGlu-His-Trp which showed 1% of the decapeptide in one of four experiments. Neither [Ser(But)4]-LH-RH-nor [Leu4]-LH-RH showed significant activity indicating (despite the known activity of [Ala4]-LH-RH) the importance of this part of the structure for full biological activity.", "contents": "Synthesis and biological activity of luteinizing hormone-releasing hormone and related peptides. Syntheses of the decapeptide luteinizing hormone-releasing hormone, less thanGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 are described. The basic properties of arginine can provide a simple repetitive isolation procedure for arginine-containing peptides. The biological activities of the decapeptide, of a range of fragments and modified fragments, and of two analogs with alteration in the series at position 4 were measured by in vitro incubation with sheep pituitary slices, measuring the liberated LH by bioassay. None of the compounds of shortened sequence were active, with the exception of less thanGlu-His-Trp which showed 1% of the decapeptide in one of four experiments. Neither [Ser(But)4]-LH-RH-nor [Leu4]-LH-RH showed significant activity indicating (despite the known activity of [Ala4]-LH-RH) the importance of this part of the structure for full biological activity."}
{"id": "PMID:1097691", "title": "7-N-amidinocephalosporins.", "content": "7-Aminocephalosporanic acid tert-butyl ester reacts quantitatively at--20 degrees with iminium chlorides to give amidino derivatives. Removal of the tert-butyl protecting group with trifluoroacetic acid and treatment with 1 equiv of triethylamine yield the corresponding zwitterions. These compounds were less active than their penicillin analogs.", "contents": "7-N-amidinocephalosporins. 7-Aminocephalosporanic acid tert-butyl ester reacts quantitatively at--20 degrees with iminium chlorides to give amidino derivatives. Removal of the tert-butyl protecting group with trifluoroacetic acid and treatment with 1 equiv of triethylamine yield the corresponding zwitterions. These compounds were less active than their penicillin analogs."}
{"id": "PMID:1097692", "title": "Synthesis and biological activity of some antitumor benzophenanthridinium salts.", "content": "A facil synthesis of benzophenanthridinium salts has been developed and used for preparing a number of these compounds.  The antitumor activities in mouse leukemia L1210 (LE) and P388 (PS) were determined as well as some selected antimicrobial activities.  Although antitumor activity was exhibited by several of the derivatives, none was as active as the naturally occurring alkaloid fagaronine.  Fagaronine was made available as a synthetic by an improved procedure.  Some structure-activity relationships among antitumor benzophenanthridinium salts are discussed.", "contents": "Synthesis and biological activity of some antitumor benzophenanthridinium salts. A facil synthesis of benzophenanthridinium salts has been developed and used for preparing a number of these compounds.  The antitumor activities in mouse leukemia L1210 (LE) and P388 (PS) were determined as well as some selected antimicrobial activities.  Although antitumor activity was exhibited by several of the derivatives, none was as active as the naturally occurring alkaloid fagaronine.  Fagaronine was made available as a synthetic by an improved procedure.  Some structure-activity relationships among antitumor benzophenanthridinium salts are discussed."}
{"id": "PMID:1097693", "title": "Quinoxaline studies. 23. Potential antimalarials. Substituted 5,8-dimethoxyquinoxalines.", "content": "Two series of 2,3-disubstituted 5,8-dimethoxy-6-[N- (omega-dimethylaminoalkyl) amino] quinoxalines were prepared: the first series with identical 2,3-substituents H, CH3, C6H5, C6H4-4-Cl, and CH2C6H5; and the second with identical styryl groups CH=CHC6H5, CH=CHC6H4-4-Cl, CH=CHC6H3-3,4-C12, CH=CHC6H4-4-F, CH=CHC6H4-4-CF3, and CH=CHC6H4-4-NO2. None of the substances possessed antimalarial activity; several were toxic at highest dosage levels.", "contents": "Quinoxaline studies. 23. Potential antimalarials. Substituted 5,8-dimethoxyquinoxalines. Two series of 2,3-disubstituted 5,8-dimethoxy-6-[N- (omega-dimethylaminoalkyl) amino] quinoxalines were prepared: the first series with identical 2,3-substituents H, CH3, C6H5, C6H4-4-Cl, and CH2C6H5; and the second with identical styryl groups CH=CHC6H5, CH=CHC6H4-4-Cl, CH=CHC6H3-3,4-C12, CH=CHC6H4-4-F, CH=CHC6H4-4-CF3, and CH=CHC6H4-4-NO2. None of the substances possessed antimalarial activity; several were toxic at highest dosage levels."}
{"id": "PMID:1097694", "title": "Synthesis and antibacterial activities of some chloro analogs of 3 amino-3,4-dihydro-1-hydroxycarbostyril.", "content": "The effects of a chloro substituent upon the microbiological activities of 3-amino-3,4-dihydro-1-hydroxycarbostyril were determined. The 5-, 6-, and 7-chloro analogs were synthesized by reductive cyclizations of the appropriately chloro-substituted o-nitrophenylalanines, while the 8-chloro analog was obtained from the N-trifluoroacetyl-3-chloro-2-nitrophenylalanine ethyl ester. All of these compounds were observed to inhibit the growth of Escherichia coli 9723, Leuconostoc dextranicum 8086, and Lactobacillus plantarum 8014. The relative inhibitory activities of the chloro analogs were 7-Cl greater than 6-Cl greater than 8-Cl greater than 5-Cl in E. coli and 7-Cl greater than 6-Cl greater than 8-Cl = 5-Cl in L. dextranicum and L. plantarum. In each of the three microorganisms, the 7-Cl analog was a more effective growth inhibitor than the parent unsubstituted compound. The growth inhibitory activities of this class of compounds were demonstrated to be much more effective than those of the four corresponding lactams, the 5-, 6-, 7-, and 8-chloro analogs of 3-amino-3,4-dihydrocarbostyril.", "contents": "Synthesis and antibacterial activities of some chloro analogs of 3 amino-3,4-dihydro-1-hydroxycarbostyril. The effects of a chloro substituent upon the microbiological activities of 3-amino-3,4-dihydro-1-hydroxycarbostyril were determined. The 5-, 6-, and 7-chloro analogs were synthesized by reductive cyclizations of the appropriately chloro-substituted o-nitrophenylalanines, while the 8-chloro analog was obtained from the N-trifluoroacetyl-3-chloro-2-nitrophenylalanine ethyl ester. All of these compounds were observed to inhibit the growth of Escherichia coli 9723, Leuconostoc dextranicum 8086, and Lactobacillus plantarum 8014. The relative inhibitory activities of the chloro analogs were 7-Cl greater than 6-Cl greater than 8-Cl greater than 5-Cl in E. coli and 7-Cl greater than 6-Cl greater than 8-Cl = 5-Cl in L. dextranicum and L. plantarum. In each of the three microorganisms, the 7-Cl analog was a more effective growth inhibitor than the parent unsubstituted compound. The growth inhibitory activities of this class of compounds were demonstrated to be much more effective than those of the four corresponding lactams, the 5-, 6-, 7-, and 8-chloro analogs of 3-amino-3,4-dihydrocarbostyril."}
{"id": "PMID:1097720", "title": "Mafenide acetate solution dressings: an adjunct in burn wound care.", "content": "A continuation of the study of 5% aqueous Sulfamylon solution dressings in burned patients was analyzed in 150 consecutive cases. The rate of invasive infection and mortality was not excessive. Dressings were used as an adjunct to other topical chemotherapeutic agents as well as homo/heterograft skin in the overall burn care program. Sulfamylon soaks were shown to be effective for debridement, granulation tissue protection and preparation, and bacterial control. The dressings were comfortable when in place and the wounds appeared clean. Epithelialization was not hampered so that the dressings could be utilized in partial thickness wounds as well as for mesh autografts on extensive burn surfaces=", "contents": "Mafenide acetate solution dressings: an adjunct in burn wound care. A continuation of the study of 5% aqueous Sulfamylon solution dressings in burned patients was analyzed in 150 consecutive cases. The rate of invasive infection and mortality was not excessive. Dressings were used as an adjunct to other topical chemotherapeutic agents as well as homo/heterograft skin in the overall burn care program. Sulfamylon soaks were shown to be effective for debridement, granulation tissue protection and preparation, and bacterial control. The dressings were comfortable when in place and the wounds appeared clean. Epithelialization was not hampered so that the dressings could be utilized in partial thickness wounds as well as for mesh autografts on extensive burn surfaces="}
{"id": "PMID:1097721", "title": "Acute common femoral arterial trauma.", "content": "1) Acute common femoral arterial injuries remain relatively uncommon in both military and civilian practice. 2) The recent experience in Vietnam has provided the most complete statistics regarding this type of injury. Among 1,000 acute major arterial injuries, the incidence of common femoral arterial trauma was approximately 5%. This present study reports 109 acute common femoral arterial injuries. Nearly two thirds of the injuries were caused by fragments and slightly more than one half of the injuries were lacerations. It was possible to perform autogenous vein graft repairs in about 57%, end-to-end anastomoses in about 25%, and lateral suture repair in about 10% of the injuries. Prosthetic material was used in only three repairs. 3) The overall complication rate was 28.3%, the amputation rate was 13.2%, and the mortality rate was 2.8%. 4) In the treatment of iatrogenic injuries associated with diagnostic catheterizations of the common femoral artery at Walter Reed Army Medical Center, there were no complications and no mortalities.", "contents": "Acute common femoral arterial trauma. 1) Acute common femoral arterial injuries remain relatively uncommon in both military and civilian practice. 2) The recent experience in Vietnam has provided the most complete statistics regarding this type of injury. Among 1,000 acute major arterial injuries, the incidence of common femoral arterial trauma was approximately 5%. This present study reports 109 acute common femoral arterial injuries. Nearly two thirds of the injuries were caused by fragments and slightly more than one half of the injuries were lacerations. It was possible to perform autogenous vein graft repairs in about 57%, end-to-end anastomoses in about 25%, and lateral suture repair in about 10% of the injuries. Prosthetic material was used in only three repairs. 3) The overall complication rate was 28.3%, the amputation rate was 13.2%, and the mortality rate was 2.8%. 4) In the treatment of iatrogenic injuries associated with diagnostic catheterizations of the common femoral artery at Walter Reed Army Medical Center, there were no complications and no mortalities."}
{"id": "PMID:1097722", "title": "Penetrating abdominal injuries in children and adolescents.", "content": "One hundred thirty-two children and adolescents with penetrating abdominal trauma have been reported. Eighty-two of these patients had intraperitoneal injuries. Five of the injured patients died, and 35 suffered complications. Patterns of organ injuries were found to be similar to a previously studied adult series. Satisfactory results were obtained by simple management of specific injuries. Ninety-five per cent of the injured patients and 80% of uninjured patients could be accurately identified at initial physical examination. Selective observation of penetrating abdominal injuries can be safely employed in children and adolescents.", "contents": "Penetrating abdominal injuries in children and adolescents. One hundred thirty-two children and adolescents with penetrating abdominal trauma have been reported. Eighty-two of these patients had intraperitoneal injuries. Five of the injured patients died, and 35 suffered complications. Patterns of organ injuries were found to be similar to a previously studied adult series. Satisfactory results were obtained by simple management of specific injuries. Ninety-five per cent of the injured patients and 80% of uninjured patients could be accurately identified at initial physical examination. Selective observation of penetrating abdominal injuries can be safely employed in children and adolescents."}
{"id": "PMID:1097723", "title": "Advantages of double releases in axillary, antecubital and popliteal contractures.", "content": "The advantages of the double release technique in axillary contractures are discussed. The procedure is useful for releasing contractures involving the antecubital and popliteal spaces as well. The risk of cutting vital structures involved in the scar tissue is minimized; also, the skin in the center of the axilla, if infected or ruptured, can be covered without as much threat to the axillary space. In addition, by placing the skin grafts on the surfaces at edges of the antecubital or in the popliteal space, the take of the skin graft on a flat surface is better insured.", "contents": "Advantages of double releases in axillary, antecubital and popliteal contractures. The advantages of the double release technique in axillary contractures are discussed. The procedure is useful for releasing contractures involving the antecubital and popliteal spaces as well. The risk of cutting vital structures involved in the scar tissue is minimized; also, the skin in the center of the axilla, if infected or ruptured, can be covered without as much threat to the axillary space. In addition, by placing the skin grafts on the surfaces at edges of the antecubital or in the popliteal space, the take of the skin graft on a flat surface is better insured."}
{"id": "PMID:1097728", "title": "[Incomplete duplication of the urethra with intra-rectal opening of the posterior chanel (author's transl)].", "content": "We have described two cases of urethro-rectal fistulae: outstanding by their absence of anorectal abnormality. This type of malformation must be separated from the fistula observed in imperforated anus. We consider it as an incomplete duplication of the urethra; the posterior part ending in the rectum: this is a very rare abnormality. The treatment involves a separation of the posterior urethra from the rectum to bring it up to the preneum then continue as in an hypospadias usig the Duplay and Leveuf (Cecil) techniques. Seemingly normal urethra is hypoplastic in part or entirely.", "contents": "[Incomplete duplication of the urethra with intra-rectal opening of the posterior chanel (author's transl)]. We have described two cases of urethro-rectal fistulae: outstanding by their absence of anorectal abnormality. This type of malformation must be separated from the fistula observed in imperforated anus. We consider it as an incomplete duplication of the urethra; the posterior part ending in the rectum: this is a very rare abnormality. The treatment involves a separation of the posterior urethra from the rectum to bring it up to the preneum then continue as in an hypospadias usig the Duplay and Leveuf (Cecil) techniques. Seemingly normal urethra is hypoplastic in part or entirely."}
{"id": "PMID:1097729", "title": "[Treatment of infra-vesical obstructions in neurologic bladders. 27 cases].", "content": "Of a total of 176 cases of spina bifida observed from a urological standpoint, we detected an infra-vesical obstruction in 27, the diagnosis being based upon urological findings alone: dysuria, residue and infection. The 11 boys had a disorder of the striated sphincter justifying sphincterotomy, the technique and results of which are discussed, in terms of symptoms and signs resulting from the obstruction, with respect to continence and the effects on the upper tract. 16 of our patients were female and the nature of the infra-vesical obstruction was far more uncertain. A disorder of the striated sphincter was present in the girl treated by a Y-V plasty of the anterior aspect of the bladder neck passing low down on the anterior aspect of the urethra. We also present results relating to residue, retention, dysuria, continence and the upper urinary tract. Overall, these results are too recent and difficult and no definite conclusions may be drawn.", "contents": "[Treatment of infra-vesical obstructions in neurologic bladders. 27 cases]. Of a total of 176 cases of spina bifida observed from a urological standpoint, we detected an infra-vesical obstruction in 27, the diagnosis being based upon urological findings alone: dysuria, residue and infection. The 11 boys had a disorder of the striated sphincter justifying sphincterotomy, the technique and results of which are discussed, in terms of symptoms and signs resulting from the obstruction, with respect to continence and the effects on the upper tract. 16 of our patients were female and the nature of the infra-vesical obstruction was far more uncertain. A disorder of the striated sphincter was present in the girl treated by a Y-V plasty of the anterior aspect of the bladder neck passing low down on the anterior aspect of the urethra. We also present results relating to residue, retention, dysuria, continence and the upper urinary tract. Overall, these results are too recent and difficult and no definite conclusions may be drawn."}
{"id": "PMID:1097731", "title": "Polyamines in bacteriophage R17 and its RNA.", "content": "Bacteriophage R17 and its RNA were found to contain significant amounts of spermidine but not of putrescine. When isolated at 0.01 M KCl, up to 1,000 molecules of spermidine were associated with the virion. The phage RNA isolated with phenol plus sodium lauryl sulfate contained approximately 70 to 90 molecules of spermidine. The association appeared to be ionic because the bound spermidine could be dissociated by KCl, MgCl2, or both. Effects of polyamines on in vitro translation were studied using both poly(U) and phage R17-RNA as mRNA. Addition of spermidine to the system at suboptimal concentrations of Mg2+ resulted in marked stimulations of the rate of protein synthesis. Putrescine alone had no effect but stimulated the incorporation in the presence of suboptimal concentrations of spermidine plus Mg2+. The isolated amino acid-incorporating system contained suboptimal soluble and bound polyamines. A comparison of incorporation was made in this system using R17-RNA with and without bound spermidine. No effects of these bound cations were detected on the rate or extent of incorporation of valine. The ratio of incorporation of histidine (present in non-coat proteins) to valine (total protein) revealed little difference as a functions of cation in the system or a function of the spermidine present in R17-RNA.", "contents": "Polyamines in bacteriophage R17 and its RNA. Bacteriophage R17 and its RNA were found to contain significant amounts of spermidine but not of putrescine. When isolated at 0.01 M KCl, up to 1,000 molecules of spermidine were associated with the virion. The phage RNA isolated with phenol plus sodium lauryl sulfate contained approximately 70 to 90 molecules of spermidine. The association appeared to be ionic because the bound spermidine could be dissociated by KCl, MgCl2, or both. Effects of polyamines on in vitro translation were studied using both poly(U) and phage R17-RNA as mRNA. Addition of spermidine to the system at suboptimal concentrations of Mg2+ resulted in marked stimulations of the rate of protein synthesis. Putrescine alone had no effect but stimulated the incorporation in the presence of suboptimal concentrations of spermidine plus Mg2+. The isolated amino acid-incorporating system contained suboptimal soluble and bound polyamines. A comparison of incorporation was made in this system using R17-RNA with and without bound spermidine. No effects of these bound cations were detected on the rate or extent of incorporation of valine. The ratio of incorporation of histidine (present in non-coat proteins) to valine (total protein) revealed little difference as a functions of cation in the system or a function of the spermidine present in R17-RNA."}
{"id": "PMID:1097732", "title": "Effect of UV irradiation on transduction by coliphage T1.", "content": "Lysates of the virulent coliphage T1 transduce seven markers between strains of Escherichia coli with reproducilble efficiencies which range from 10(-7) to 10(-5). The ability of a UV-irradiated lysate to transduce Arg(+), Str(R), Trp(+), Lac(+), and Pro(+) is 90% and Bio(+) is 99% inactivated by doses which inactivate plaque formation of T1 by six orders of magnitude. A dose of irradiation which causes a 1- to 2-log drop in the titer of T1 stimulated Gal(+) transduction by two- to three-fold; no other marker tested was stimulated. Irradiation causes dislinkage of some co-transducible markers but not others.", "contents": "Effect of UV irradiation on transduction by coliphage T1. Lysates of the virulent coliphage T1 transduce seven markers between strains of Escherichia coli with reproducilble efficiencies which range from 10(-7) to 10(-5). The ability of a UV-irradiated lysate to transduce Arg(+), Str(R), Trp(+), Lac(+), and Pro(+) is 90% and Bio(+) is 99% inactivated by doses which inactivate plaque formation of T1 by six orders of magnitude. A dose of irradiation which causes a 1- to 2-log drop in the titer of T1 stimulated Gal(+) transduction by two- to three-fold; no other marker tested was stimulated. Irradiation causes dislinkage of some co-transducible markers but not others."}
{"id": "PMID:1097733", "title": "Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli.", "content": "The dnaB gene product of Escherischia coli is required for multiplication of temperate phage P2. At 37 C in dnaB-ts mutnats, P2 will not plaque and gives a very small burst of progeny. P2 mutants have been isolated which can grow well enough to plaque under these conditions. This type of phage mutant is cis dominant, and one such mutant (P2rlb1) has been mapped near the left end of the early gene B and to the right of the cox4 (excision) mutation. The rlb1 mutation does not lie at the replication origin, but may affect transcription in the early region, which includes the replication origin. It may also represent a site on the P2 DNA which interacts with the dnaB gene product.", "contents": "Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli. The dnaB gene product of Escherischia coli is required for multiplication of temperate phage P2. At 37 C in dnaB-ts mutnats, P2 will not plaque and gives a very small burst of progeny. P2 mutants have been isolated which can grow well enough to plaque under these conditions. This type of phage mutant is cis dominant, and one such mutant (P2rlb1) has been mapped near the left end of the early gene B and to the right of the cox4 (excision) mutation. The rlb1 mutation does not lie at the replication origin, but may affect transcription in the early region, which includes the replication origin. It may also represent a site on the P2 DNA which interacts with the dnaB gene product."}
{"id": "PMID:1097734", "title": "Discriminative effect of rifampin of RNA replication of various RNA bacteriophages.", "content": "Rifampin interferes exclusively with RNA replication in vivo of the group I phages MS2, f2, and R17, whereas QbetaRNA replication is unaffected by the drug. In addition, rifampin has a discriminative effect of group I phage RNA replication. In the experimental system employed by us the antibiotic differentially interferes with the synthesis of minus RNA strands in f2, whereas it has almost no effect on the synthesis of progeny plus strands. In MS2, the drug differentially arrests the synthesis of progeny plus strands and almost fails to affect the synthesis of minus RNA strands. In R17 both steps of its RNA replication are affected by rifampin, although each step is only partially (approximately 50%) inhibited. The relation of the present results to the possible role of bacterial proteins and tertiary structure of phage  RNA in the process of template recognition is discussed.", "contents": "Discriminative effect of rifampin of RNA replication of various RNA bacteriophages. Rifampin interferes exclusively with RNA replication in vivo of the group I phages MS2, f2, and R17, whereas QbetaRNA replication is unaffected by the drug. In addition, rifampin has a discriminative effect of group I phage RNA replication. In the experimental system employed by us the antibiotic differentially interferes with the synthesis of minus RNA strands in f2, whereas it has almost no effect on the synthesis of progeny plus strands. In MS2, the drug differentially arrests the synthesis of progeny plus strands and almost fails to affect the synthesis of minus RNA strands. In R17 both steps of its RNA replication are affected by rifampin, although each step is only partially (approximately 50%) inhibited. The relation of the present results to the possible role of bacterial proteins and tertiary structure of phage  RNA in the process of template recognition is discussed."}
{"id": "PMID:1097735", "title": "Replication of bacteriophage M13 IX. Requirement of the Escherichia coli dnaG function for M13 duplex DNA replication.", "content": "Temperature-shift experiments with an Escherichia coli dnaG strain indicate a requirement for the dnaG function for M13 phage production only at an early stage of infection. Mutant cells infected at nonpermissive temperature form the parental RF (SS leads to RF) but do not replicate further. A shift to nonpermissive temperature after infection inhibits RF leads to RF replication but not RF leads to SS synthesis. The synthesis of both strands of the duplex RF was inhibited equally after a temperature shift during RF leads to RF replication. We infer that the dnaG protein is required for M13 production only during RF replication and that it is required for the synthesis of both strands of the RF.", "contents": "Replication of bacteriophage M13 IX. Requirement of the Escherichia coli dnaG function for M13 duplex DNA replication. Temperature-shift experiments with an Escherichia coli dnaG strain indicate a requirement for the dnaG function for M13 phage production only at an early stage of infection. Mutant cells infected at nonpermissive temperature form the parental RF (SS leads to RF) but do not replicate further. A shift to nonpermissive temperature after infection inhibits RF leads to RF replication but not RF leads to SS synthesis. The synthesis of both strands of the duplex RF was inhibited equally after a temperature shift during RF leads to RF replication. We infer that the dnaG protein is required for M13 production only during RF replication and that it is required for the synthesis of both strands of the RF."}
{"id": "PMID:1097736", "title": "Synthesis of complex forms of bacteriophage phiX174 double-stranded DNA in a temperature-sensitive dnaC mutant of Escherichia coli C.", "content": "Fast-sedimenting forms of bacteriophage phiX174 double-stranded replicative-form DNA observed in normal infections continued to accumulate at the nonpermissive temperature in a temperature-sensitive dnaC mutant of Escherichia coli. These complex molecules accounted for up to half of the DNA synthesized during short pulses at the nonpermissive temperature. They were the dead-end products of DNA synthesis, not intermediates in normal replicative-form replication. The data suggest that these higher-than-normal-molecular-weight DNA molecules result from abnormal initiation of phiX174 replicative-form DNA replication.", "contents": "Synthesis of complex forms of bacteriophage phiX174 double-stranded DNA in a temperature-sensitive dnaC mutant of Escherichia coli C. Fast-sedimenting forms of bacteriophage phiX174 double-stranded replicative-form DNA observed in normal infections continued to accumulate at the nonpermissive temperature in a temperature-sensitive dnaC mutant of Escherichia coli. These complex molecules accounted for up to half of the DNA synthesized during short pulses at the nonpermissive temperature. They were the dead-end products of DNA synthesis, not intermediates in normal replicative-form replication. The data suggest that these higher-than-normal-molecular-weight DNA molecules result from abnormal initiation of phiX174 replicative-form DNA replication."}
{"id": "PMID:1097737", "title": "Biological functions of the bacteriophage T3 SAMase gene.", "content": "Certain differences between phage T3 on the one hand and T3sam- and T7 on the other hand indicate that the T3-coded SAMase function is responsible (i) for the development of the pseudolysogenic state by preventing T3 DNA methylation, and (ii) for the partial protection of the phage DNA against restriction by the P system.", "contents": "Biological functions of the bacteriophage T3 SAMase gene. Certain differences between phage T3 on the one hand and T3sam- and T7 on the other hand indicate that the T3-coded SAMase function is responsible (i) for the development of the pseudolysogenic state by preventing T3 DNA methylation, and (ii) for the partial protection of the phage DNA against restriction by the P system."}
{"id": "PMID:1097738", "title": "Lysozymes from bacteriophages T3 and T5.", "content": "Lysozymes produced in host cells infected with bacteriophages T3 and T5 were found to have the same enzymatic specificity toward the peptidoglycan from Escherichia coli as T7 phage lysozyme, which has been shown to be an N-acetylmuramyl-L-alanine amidase.", "contents": "Lysozymes from bacteriophages T3 and T5. Lysozymes produced in host cells infected with bacteriophages T3 and T5 were found to have the same enzymatic specificity toward the peptidoglycan from Escherichia coli as T7 phage lysozyme, which has been shown to be an N-acetylmuramyl-L-alanine amidase."}
{"id": "PMID:1097739", "title": "Renal autotransplantation using hypothermic storage and pulsatile perfusion.", "content": "Three patients with severe hypertension secondary to renal artery stenosis were treated by renal autotransplantation. Of these 3 patients 2 had solitary kidneys and 1 had 2 renal arteries to each kidney, all of which were stenosed. Renal autotransplantation with hypothermia of the kidney was performed in all 3 patients rather than the more conventional arterial bypass or endarterectomy because 1) hypothermic preservation permitted a prolonged ischemia time and 2) there was improved exposure for the vascular anastomosis. Postoperatively 2 patients remained normotensive without drugs for 9 and 12 months and 1 patient died of septicemia not directly related to the autotransplant. All 3 patients required expansion of the intravascular volume postoperatively to overcome the loss of vasoconstrictor substances following restoration of renal blood flow.", "contents": "Renal autotransplantation using hypothermic storage and pulsatile perfusion. Three patients with severe hypertension secondary to renal artery stenosis were treated by renal autotransplantation. Of these 3 patients 2 had solitary kidneys and 1 had 2 renal arteries to each kidney, all of which were stenosed. Renal autotransplantation with hypothermia of the kidney was performed in all 3 patients rather than the more conventional arterial bypass or endarterectomy because 1) hypothermic preservation permitted a prolonged ischemia time and 2) there was improved exposure for the vascular anastomosis. Postoperatively 2 patients remained normotensive without drugs for 9 and 12 months and 1 patient died of septicemia not directly related to the autotransplant. All 3 patients required expansion of the intravascular volume postoperatively to overcome the loss of vasoconstrictor substances following restoration of renal blood flow."}
{"id": "PMID:1097740", "title": "Medrogestone: a prospective study in the pharmaceutical management of benign prostatic hyperplasia.", "content": "Medrogestone is a progestational agent that may reduce outflow obstruction secondary to benign prostatic hyperplasia. Five of 8 patients taking 15 mg. oral medrogestone twice daily and 5 of 7 patients taking 7.5 mg. oral medrogestone twice daily experienced an increase in mean urine flow (averaging 55.8 and 30.1 per cent, respectively), while no change was noted in 4 untreated patients. Five of the 8 patients taking 15 mg. and 6 of the 7 patients taking 7.5 mg. experienced an increase in peak urine flow (averaging 60 and 37.6 per cent, respectively), while 2 of the 4 controls had an improvement of only 12.2 per cent. Repetitive residual urines and digital examination were inaccurate to assay sequential changes in prostatic size. Certain side effects, such as hypertension, abnormal glucose tolerance and impotence, were encountered in isolated patients but in no instance was the side effect hazardous to the health of the test subject and in each instance it could be reversed by withdrawal of the medication.", "contents": "Medrogestone: a prospective study in the pharmaceutical management of benign prostatic hyperplasia. Medrogestone is a progestational agent that may reduce outflow obstruction secondary to benign prostatic hyperplasia. Five of 8 patients taking 15 mg. oral medrogestone twice daily and 5 of 7 patients taking 7.5 mg. oral medrogestone twice daily experienced an increase in mean urine flow (averaging 55.8 and 30.1 per cent, respectively), while no change was noted in 4 untreated patients. Five of the 8 patients taking 15 mg. and 6 of the 7 patients taking 7.5 mg. experienced an increase in peak urine flow (averaging 60 and 37.6 per cent, respectively), while 2 of the 4 controls had an improvement of only 12.2 per cent. Repetitive residual urines and digital examination were inaccurate to assay sequential changes in prostatic size. Certain side effects, such as hypertension, abnormal glucose tolerance and impotence, were encountered in isolated patients but in no instance was the side effect hazardous to the health of the test subject and in each instance it could be reversed by withdrawal of the medication."}
{"id": "PMID:1097741", "title": "Seminoma in a renal transplant recipient.", "content": "A case is reported of testicular seminoma developing de novo 13 months post-transplantation. Two other post-transplant scrotal complications were noted, that is hydrocele and testicular atrophy. The question raised is what type of therapy would be best for this patient.", "contents": "Seminoma in a renal transplant recipient. A case is reported of testicular seminoma developing de novo 13 months post-transplantation. Two other post-transplant scrotal complications were noted, that is hydrocele and testicular atrophy. The question raised is what type of therapy would be best for this patient."}
{"id": "PMID:1097742", "title": "Studies on the oral infectivity of rabies virus in carnivora.", "content": "Mature and immature red foxes (Vulpes vulpes) and striped skunks (Mephitis mephitis) were fed varying numbers of white mice infected with street isolates and a fixed strain of rabies virus. Rabies deaths and the development of serum neutralizing antibody to rabies virus occurred in both species. The epizootiological implications of these findings are discussed.", "contents": "Studies on the oral infectivity of rabies virus in carnivora. Mature and immature red foxes (Vulpes vulpes) and striped skunks (Mephitis mephitis) were fed varying numbers of white mice infected with street isolates and a fixed strain of rabies virus. Rabies deaths and the development of serum neutralizing antibody to rabies virus occurred in both species. The epizootiological implications of these findings are discussed."}
{"id": "PMID:1097743", "title": "Vaccination of foxes against rabies using ingested baits.", "content": "A method for immunizing foxes against rabies was evaluated. Fifteen of 36 red foxes (Vulpes fulva) fed baits impregnated with modified live virus rabies vaccine developed serum rabies neutralizing antibody. The vaccine-bait proved unstable when held at 4 C or 25 C for 96 hours prior to feeding. For safety testing the vaccine virus was administered to opossums (Didelphis virginiana), cotton rats (Sigmodon hispidus), hamsters (Mesocricetus auratus), and chickens (Gallus domesticus) in either liquid or bait form. One of ten cotton rats fed liquid vaccine died of vaccine induced rabies. No animals which ate the vaccine-baits died of vaccine induced rabies.", "contents": "Vaccination of foxes against rabies using ingested baits. A method for immunizing foxes against rabies was evaluated. Fifteen of 36 red foxes (Vulpes fulva) fed baits impregnated with modified live virus rabies vaccine developed serum rabies neutralizing antibody. The vaccine-bait proved unstable when held at 4 C or 25 C for 96 hours prior to feeding. For safety testing the vaccine virus was administered to opossums (Didelphis virginiana), cotton rats (Sigmodon hispidus), hamsters (Mesocricetus auratus), and chickens (Gallus domesticus) in either liquid or bait form. One of ten cotton rats fed liquid vaccine died of vaccine induced rabies. No animals which ate the vaccine-baits died of vaccine induced rabies."}
{"id": "PMID:1097744", "title": "Serologic observations during an outbreak of rat borne plague in the San Francisco Bay area of California.", "content": "Results of a serologic study of a plaque outbreak among rats (Rattus norvegicus) and associated wild rodents are presented. Bacteriologic and serologic evidence points toward mutual exchange of fleas and plaque infection between the intermingled rat and wild rodent populations. Results emphasize the value of serologic methods for epizootiologic studies of plague in North American rat populations.", "contents": "Serologic observations during an outbreak of rat borne plague in the San Francisco Bay area of California. Results of a serologic study of a plaque outbreak among rats (Rattus norvegicus) and associated wild rodents are presented. Bacteriologic and serologic evidence points toward mutual exchange of fleas and plaque infection between the intermingled rat and wild rodent populations. Results emphasize the value of serologic methods for epizootiologic studies of plague in North American rat populations."}
{"id": "PMID:1097745", "title": "Bacteriuria in the catheterized patient.  Cystitis or pyelonephritis?", "content": "Seventy-two patients with indwelling urethral catheters had urine specimens cultured to detect bacteriuria.  Bacterial sediments were examined to find the source of the bacteria, with use of an antibody immunofluorescence (IF) technique.  Antibody coating demonstrated by fluorescence was considered evidence for pyelonephritis.  In cystitis, the bacteria did not fluoresce.  Repeat culture and immunofluorescence studies were done 3 to 18 week later.  One third of the patients had a urinary infection.  Two (13%) of the patients catheterized for one day and 17 (55%) catheterized four or more days had appreciable bacteriuria.  One patient catheterized less than three days had positive IF, while 5 patients (16%) catheterized four or more days had positive IF.  Only two of the ten patients observed, with an initial infection, had sterile urine on follow-up culture.  On follow-up study, of the four patients with bacteriuria of renal origin, one showed a sterile urine, two had cystitis, and one still had pyelonephritis.", "contents": "Bacteriuria in the catheterized patient.  Cystitis or pyelonephritis? Seventy-two patients with indwelling urethral catheters had urine specimens cultured to detect bacteriuria.  Bacterial sediments were examined to find the source of the bacteria, with use of an antibody immunofluorescence (IF) technique.  Antibody coating demonstrated by fluorescence was considered evidence for pyelonephritis.  In cystitis, the bacteria did not fluoresce.  Repeat culture and immunofluorescence studies were done 3 to 18 week later.  One third of the patients had a urinary infection.  Two (13%) of the patients catheterized for one day and 17 (55%) catheterized four or more days had appreciable bacteriuria.  One patient catheterized less than three days had positive IF, while 5 patients (16%) catheterized four or more days had positive IF.  Only two of the ten patients observed, with an initial infection, had sterile urine on follow-up culture.  On follow-up study, of the four patients with bacteriuria of renal origin, one showed a sterile urine, two had cystitis, and one still had pyelonephritis."}
{"id": "PMID:1097747", "title": "Trimethoprim-sulfamethoxazole vs ampicillin in chronic urinary tract infections. A double-blind multicenter cooperative controlled study.", "content": "A multicenter, prospective, double-blind, controlled study was performed to compare the efficacy of trimethoprim-sulfamethoxazole with that of ampicillin in the treatment of chronic urinary tract infections. The incidence of adverse clinical signs and symptoms as well as abnormal laboratory values were comparable in the two treatment groups. This study appears to establish the fact that trimethoprim-sulfamethoxazole is an acceptable alternative to ampicillin for the treatment of chronic urinary tract infections caused by susceptible Escherichia coli.", "contents": "Trimethoprim-sulfamethoxazole vs ampicillin in chronic urinary tract infections. A double-blind multicenter cooperative controlled study. A multicenter, prospective, double-blind, controlled study was performed to compare the efficacy of trimethoprim-sulfamethoxazole with that of ampicillin in the treatment of chronic urinary tract infections. The incidence of adverse clinical signs and symptoms as well as abnormal laboratory values were comparable in the two treatment groups. This study appears to establish the fact that trimethoprim-sulfamethoxazole is an acceptable alternative to ampicillin for the treatment of chronic urinary tract infections caused by susceptible Escherichia coli."}
{"id": "PMID:1097749", "title": "The 12th Report of the Human Renal Transplant Registry. Prepared by the Advisory Committee to the Renal Transplant Registry.", "content": "This report, the 12th, from the Renal Transplant Registry summarizes current results of kidney transplantation in man. There has been a trend toward increasing use of cadaveric sources for kidney transplantation. Results are summarized in five-year life-table analyses of recipient survival and graft function. Also, a summary of the results of special studies is provided. These include preparation of the recipient for transplantation by nephrectomy in various disease states, comparison of simple flushing with machine perfusion in cadaveric kidney storage, results of transplantation in the pediatric recipient, and the development of malignant neoplasm in graft recipients. A large study relating typing to graft function presents information at some variance from previous reports published in the United States.", "contents": "The 12th Report of the Human Renal Transplant Registry. Prepared by the Advisory Committee to the Renal Transplant Registry. This report, the 12th, from the Renal Transplant Registry summarizes current results of kidney transplantation in man. There has been a trend toward increasing use of cadaveric sources for kidney transplantation. Results are summarized in five-year life-table analyses of recipient survival and graft function. Also, a summary of the results of special studies is provided. These include preparation of the recipient for transplantation by nephrectomy in various disease states, comparison of simple flushing with machine perfusion in cadaveric kidney storage, results of transplantation in the pediatric recipient, and the development of malignant neoplasm in graft recipients. A large study relating typing to graft function presents information at some variance from previous reports published in the United States."}
{"id": "PMID:1097753", "title": "Factitious meningitis. Diagnostic error due to nonviable bacteria in commercial lumbar puncture trays.", "content": "A cluster of five false-positive cerebrospinal fluid (CSF) Gram stains led to an investigation of possible causes of specimen or smear contamination. Specimen tubes supplied in commercial lumbar puncture trays from the lot being used in the involved hospital were shown to contain nonviable bacteria, When filled with a test solution and processed in a manner similar to that used for processing CSF specimens, 10 of 12 tubes evaluated yielded Gram stains containing either Gram-negative rods, diplococci, or coccobacilli. Before this problem was recognized, the patient from whom the first false-positive smear was obtained was treated for bacterial meningitis. It is important to realize that microbial contamination of commercial CSF specimen tubes can result in findings simulating those of early bacterial meningitis.", "contents": "Factitious meningitis. Diagnostic error due to nonviable bacteria in commercial lumbar puncture trays. A cluster of five false-positive cerebrospinal fluid (CSF) Gram stains led to an investigation of possible causes of specimen or smear contamination. Specimen tubes supplied in commercial lumbar puncture trays from the lot being used in the involved hospital were shown to contain nonviable bacteria, When filled with a test solution and processed in a manner similar to that used for processing CSF specimens, 10 of 12 tubes evaluated yielded Gram stains containing either Gram-negative rods, diplococci, or coccobacilli. Before this problem was recognized, the patient from whom the first false-positive smear was obtained was treated for bacterial meningitis. It is important to realize that microbial contamination of commercial CSF specimen tubes can result in findings simulating those of early bacterial meningitis."}
{"id": "PMID:1097758", "title": "Quantitation of bidirectional shunt from a single dye-dilution curve.", "content": "A method for quantitating intracardiac bidirectional shunt and estimating its blood flow is presented. Three curves, defined as \"right-to-left shunt curve\", \"normal pathway curve\", and \"left-to-right shunt curve\" were separately drawn from the original downstream dye-dilution curve, and their areas ?A'', A, A', respectively, were calculated in the conventional manner. A'/A showed left-to-right shunt ratio (QL-R%) even in the presence of right-to-left shunt. However, right-to-left shunt ratio (QR-L%) was given by (1-A'/A)/(A/A'' + 1--A'/A), and equalled A''/(A + A'') only when left-to-right shunt was absent (A' equals O). A''/(A + A) should become increasingly higher than QR-L% with rise in QL-R% (equals A'/A). The relationship between A''/(A + A'') and QR-L% for various A/A isopleths is shown by figure. A good agreement (r equals 0.848, p less than 0.001) was found between QL-R% values by the present method and those calculated by the oxygen method. In contrast, QR-L% values by the present method were consistently lower than those by the oxygen method; the difference was exaggerated especially in cases with history of cardiac failure, and these cases tended to show lower systemic flow. The average difference in QR-L% between the two methods, found in cases free from failure, was 7 per cent. This was thought to be ascribed to intrapulmonary physiological shunt.", "contents": "Quantitation of bidirectional shunt from a single dye-dilution curve. A method for quantitating intracardiac bidirectional shunt and estimating its blood flow is presented. Three curves, defined as \"right-to-left shunt curve\", \"normal pathway curve\", and \"left-to-right shunt curve\" were separately drawn from the original downstream dye-dilution curve, and their areas ?A'', A, A', respectively, were calculated in the conventional manner. A'/A showed left-to-right shunt ratio (QL-R%) even in the presence of right-to-left shunt. However, right-to-left shunt ratio (QR-L%) was given by (1-A'/A)/(A/A'' + 1--A'/A), and equalled A''/(A + A'') only when left-to-right shunt was absent (A' equals O). A''/(A + A) should become increasingly higher than QR-L% with rise in QL-R% (equals A'/A). The relationship between A''/(A + A'') and QR-L% for various A/A isopleths is shown by figure. A good agreement (r equals 0.848, p less than 0.001) was found between QL-R% values by the present method and those calculated by the oxygen method. In contrast, QR-L% values by the present method were consistently lower than those by the oxygen method; the difference was exaggerated especially in cases with history of cardiac failure, and these cases tended to show lower systemic flow. The average difference in QR-L% between the two methods, found in cases free from failure, was 7 per cent. This was thought to be ascribed to intrapulmonary physiological shunt."}
{"id": "PMID:1097767", "title": "Comparative studies on generalized transducing bacteriophages of Proteus mirabilis, phim and pi1.", "content": "Comparative studies were made on the generalized transducing bacteriophages of Proteus mirabilis phim (Nakaya and Rownd), pi1(B\u00f6hme), and a clear plaque-forming mutant phim-c, derived from phim. Electron microscopic observations revealed that these phages were morphologically identical, indicating that they belonged to the group C of Bradley's classification, or to the type C1 of Ackermann's classification. Phages phim and pi1 formed characteristic turbid plaques different from each other, and the plaques of pi1 were smaller in size than those of phim. The plaques of phage phim-c were clear and also were the largest in size among those studied. Average latent periods of phim and pi1 were 70 and 60 min, respectively. Average burst size was found to be 30 and 10 plaque-forming units per infected cell for phim and pi1, respectively. It was confirmed by cross neutralization tests that phim and pi1 differed serologically  from each other. The host range of the two phages also differed, and phage phim was more sensitive to heat than pi1. These results indicate that phages phim and pi1 are different types of phages. Majority of the properties of phage phim-c were nearly identical with those of phage phim except that the multiplication of phim-c was more strongly inhibited by methylene blue than that of phim and pi1. Phage phim-c is considered to be a clear mutant of phim.", "contents": "Comparative studies on generalized transducing bacteriophages of Proteus mirabilis, phim and pi1. Comparative studies were made on the generalized transducing bacteriophages of Proteus mirabilis phim (Nakaya and Rownd), pi1(B\u00f6hme), and a clear plaque-forming mutant phim-c, derived from phim. Electron microscopic observations revealed that these phages were morphologically identical, indicating that they belonged to the group C of Bradley's classification, or to the type C1 of Ackermann's classification. Phages phim and pi1 formed characteristic turbid plaques different from each other, and the plaques of pi1 were smaller in size than those of phim. The plaques of phage phim-c were clear and also were the largest in size among those studied. Average latent periods of phim and pi1 were 70 and 60 min, respectively. Average burst size was found to be 30 and 10 plaque-forming units per infected cell for phim and pi1, respectively. It was confirmed by cross neutralization tests that phim and pi1 differed serologically  from each other. The host range of the two phages also differed, and phage phim was more sensitive to heat than pi1. These results indicate that phages phim and pi1 are different types of phages. Majority of the properties of phage phim-c were nearly identical with those of phage phim except that the multiplication of phim-c was more strongly inhibited by methylene blue than that of phim and pi1. Phage phim-c is considered to be a clear mutant of phim."}
{"id": "PMID:1097768", "title": "Nonspecific  elicitation of antibody-forming cells in the mouse spleen by bacterial lipopolysaccharide.", "content": "Mechanisms of nonspecific elicitation of anti-sheep erythrocyte (SRBC) hemolytic antibody plaqueforming cells (PFC) in mouse spleens with an injection of bacterial endotoxin (lipopolysaccharide (LPS)) were studied in comparison with the genesis of naturally occurring \"background\" PFC in normal mouse spleens and of rapidly arising PFC in mouse spleens after immunization with SRBC. The cytokinetic pattern of anti-SRBC PFC response after an injection of LPS was quite different from that of the response elicited after immunization with SRBC. In addition, even though LPS nonspecifically elicited anti-SRBC PFC response in mice, LPS could not confer any immunological memory on mouse immunocytess for a \"secondary-type\" anti-SRBC PFC response to restimulation with LPS or SRBC. The administration of rabbit anti-mouse thymocyte immunoglobulin or anti-SRBC antiserum in mice markedly suppressed the PFC response after immunization with SRBC, but did not do so after stimulation with LPS. Neonatally thymectomized mice could still respond to stimulation with LPS, producing anti-SRBC PFC in their spleens. Injections of actinomycin D or cyclophosphamide into mice resulted in obvious reductions of the PFC responses elicited by either LPS or SRBC. However, injections of these immunosuppressive antisera or drugs did not affect the number of anti-SRBC PFC in normal mouse spleens. These results suggest that the genesis of anti-SRBC PFC developed under different conditions, i.e., background PFC, LPS-stimulated PFC, and antigen-stimulated PFC, are quite different from each other, and that the nonspecific elicitation of anti-SRBC PFC by LPS does not require the helper function of T lymphocytes. No obvious difference, however, was observed in the time of ontogenic maturation among these three different anti-SRBC PFC in the mouse spleens judging from when they were first manifested after birth.", "contents": "Nonspecific  elicitation of antibody-forming cells in the mouse spleen by bacterial lipopolysaccharide. Mechanisms of nonspecific elicitation of anti-sheep erythrocyte (SRBC) hemolytic antibody plaqueforming cells (PFC) in mouse spleens with an injection of bacterial endotoxin (lipopolysaccharide (LPS)) were studied in comparison with the genesis of naturally occurring \"background\" PFC in normal mouse spleens and of rapidly arising PFC in mouse spleens after immunization with SRBC. The cytokinetic pattern of anti-SRBC PFC response after an injection of LPS was quite different from that of the response elicited after immunization with SRBC. In addition, even though LPS nonspecifically elicited anti-SRBC PFC response in mice, LPS could not confer any immunological memory on mouse immunocytess for a \"secondary-type\" anti-SRBC PFC response to restimulation with LPS or SRBC. The administration of rabbit anti-mouse thymocyte immunoglobulin or anti-SRBC antiserum in mice markedly suppressed the PFC response after immunization with SRBC, but did not do so after stimulation with LPS. Neonatally thymectomized mice could still respond to stimulation with LPS, producing anti-SRBC PFC in their spleens. Injections of actinomycin D or cyclophosphamide into mice resulted in obvious reductions of the PFC responses elicited by either LPS or SRBC. However, injections of these immunosuppressive antisera or drugs did not affect the number of anti-SRBC PFC in normal mouse spleens. These results suggest that the genesis of anti-SRBC PFC developed under different conditions, i.e., background PFC, LPS-stimulated PFC, and antigen-stimulated PFC, are quite different from each other, and that the nonspecific elicitation of anti-SRBC PFC by LPS does not require the helper function of T lymphocytes. No obvious difference, however, was observed in the time of ontogenic maturation among these three different anti-SRBC PFC in the mouse spleens judging from when they were first manifested after birth."}
{"id": "PMID:1097803", "title": "[Characteristics of the islet apparatus of the pancreas in rats with genetic spontaneous hypertension].", "content": "In spontaneously hypertensive rats (SHR) at the age of 7-9 weeks a poor development of the beta-cellular part of the islet-cell tissue was found, as compared to normotensive Wistar rats of the same age (a lower number of beta-cellular islets, decreased mass of the islet-cell tissue). With glucose load in SHR hyperglycemia was more distinct and longer lasting, which indicates the development of insulin insufficiency in them, in a situation demanding forced insulin incretion. A histochemical examination proved a reduction of the insulin content in the islet-cells of these animals, as compared with the control ones. The above mentioned changes evidently do not pertain to the primary genetically-determined pathology of their pancreas, but are of a secondary nature of atrophy of the insulin-forming tissue, that is probably conditioned by the decreased tissue requirements (target) for insulin due to the increased permeability of the cell membranes.", "contents": "[Characteristics of the islet apparatus of the pancreas in rats with genetic spontaneous hypertension]. In spontaneously hypertensive rats (SHR) at the age of 7-9 weeks a poor development of the beta-cellular part of the islet-cell tissue was found, as compared to normotensive Wistar rats of the same age (a lower number of beta-cellular islets, decreased mass of the islet-cell tissue). With glucose load in SHR hyperglycemia was more distinct and longer lasting, which indicates the development of insulin insufficiency in them, in a situation demanding forced insulin incretion. A histochemical examination proved a reduction of the insulin content in the islet-cells of these animals, as compared with the control ones. The above mentioned changes evidently do not pertain to the primary genetically-determined pathology of their pancreas, but are of a secondary nature of atrophy of the insulin-forming tissue, that is probably conditioned by the decreased tissue requirements (target) for insulin due to the increased permeability of the cell membranes."}
{"id": "PMID:1097805", "title": "Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen.", "content": "Pepsin solubilization of small and large noduled liver cirrhosis yielded two types of collagen (precipitated at 1.7 and 2.5 M NaCl concentrations) as demonstrated by electronmicroscopy. The 1.7 M NaCl precipitate was identified as type III collagen using an immunofluorescence technique. The 2.5 M NaCl precipitate appeared to be type I in the electronmicroscope. However, immunofluorescent and biochemical studies indicated that it was not type I but a type of collagen not yet described.", "contents": "Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen. Pepsin solubilization of small and large noduled liver cirrhosis yielded two types of collagen (precipitated at 1.7 and 2.5 M NaCl concentrations) as demonstrated by electronmicroscopy. The 1.7 M NaCl precipitate was identified as type III collagen using an immunofluorescence technique. The 2.5 M NaCl precipitate appeared to be type I in the electronmicroscope. However, immunofluorescent and biochemical studies indicated that it was not type I but a type of collagen not yet described."}
{"id": "PMID:1097806", "title": "[Cholestatic jaundice following disseminated intravascular coagulation (\"shock-liver\") (author's transl)].", "content": "Recently we gained increasing evidence of the fact that cholostatic syndromes in young infants are frequently associated with preceeding shock and disseminated intravascular coagulation.  This is illustrated by the case of a 4 weeks-old male infant with urosepticemia due to E. coli, which was successfully treated with additional streptokinase.  Therefore, in the age-group of early infancy we have to pay attention to the liver as a potential shock-organ similar to the well known shock organs such as kidneys, lungs, skin and brain.", "contents": "[Cholestatic jaundice following disseminated intravascular coagulation (\"shock-liver\") (author's transl)]. Recently we gained increasing evidence of the fact that cholostatic syndromes in young infants are frequently associated with preceeding shock and disseminated intravascular coagulation.  This is illustrated by the case of a 4 weeks-old male infant with urosepticemia due to E. coli, which was successfully treated with additional streptokinase.  Therefore, in the age-group of early infancy we have to pay attention to the liver as a potential shock-organ similar to the well known shock organs such as kidneys, lungs, skin and brain."}
{"id": "PMID:1097807", "title": "[The effect of caerulein on gastric mucosal blood-flow and gastric secretion (author's transl)].", "content": "The effect of Caerulein, a decapeptide produced from the skin of the Australian frog Hyla caerulea, on the mucosal blood-flow and the gastric acid secretion as well as the pepsin secretion has been investigated in 15 patients. Caerulein administered at a dose rate of 0.1 mug/kg-h significantly inhibited the gastric mucosal blood-flow (p greater than 0.005) and the acid secretion (0.05 greater than p greater than 0.01), which had been stimulated by pentagastrin at a dose rate of 1.5 mug/kg-h. The pepsin secretion stimulated with pentagastrin also decreased, but not significantly. Caerulein at the above mentioned dose rate however caused a slight but not significant increase of the acid secretion stimulated by 40 mug histamine/kg-h. At the same time, gastric mucosal blood-flow and pepsin output decreased slightly but not significantly. Caerulein given alone stimulates acid secretion, yet it causes a significant reduction of the acid output when administered together with pentagastrin. This response has to be explained by the fact, that the similar chemical structure of pentagastrin and caerulein leads to a competitive inhibition.  The mucosal blood-flow of the stomach measured with the heat-clearance technique also decreases with the administration of caerulein after pentagastrin stimulation. The fact that the mucosal blood-flow remains unchanged after the administration of histamine, suggests that the reduction in mucosal blood-flow observed with pentagastrin is a consequence and not the cause of the acid inhibition occurring at the same stage.", "contents": "[The effect of caerulein on gastric mucosal blood-flow and gastric secretion (author's transl)]. The effect of Caerulein, a decapeptide produced from the skin of the Australian frog Hyla caerulea, on the mucosal blood-flow and the gastric acid secretion as well as the pepsin secretion has been investigated in 15 patients. Caerulein administered at a dose rate of 0.1 mug/kg-h significantly inhibited the gastric mucosal blood-flow (p greater than 0.005) and the acid secretion (0.05 greater than p greater than 0.01), which had been stimulated by pentagastrin at a dose rate of 1.5 mug/kg-h. The pepsin secretion stimulated with pentagastrin also decreased, but not significantly. Caerulein at the above mentioned dose rate however caused a slight but not significant increase of the acid secretion stimulated by 40 mug histamine/kg-h. At the same time, gastric mucosal blood-flow and pepsin output decreased slightly but not significantly. Caerulein given alone stimulates acid secretion, yet it causes a significant reduction of the acid output when administered together with pentagastrin. This response has to be explained by the fact, that the similar chemical structure of pentagastrin and caerulein leads to a competitive inhibition.  The mucosal blood-flow of the stomach measured with the heat-clearance technique also decreases with the administration of caerulein after pentagastrin stimulation. The fact that the mucosal blood-flow remains unchanged after the administration of histamine, suggests that the reduction in mucosal blood-flow observed with pentagastrin is a consequence and not the cause of the acid inhibition occurring at the same stage."}
{"id": "PMID:1097823", "title": "Restraint, venipuncture, endotracheal intubation, and anesthesia of miniature swine.", "content": "Miniature swine are useful as experimental animals for investigations involving major surgical procedures, provided that proper restraint and anesthetic methods are employed. The methods and drugs described herein are not intended to be all-inclusive but rather those found most applicable to a wide variety of studies with miniature swine at this laboratory. This report is intended primarily to guide those unfamiliar in handling miniature swine during initiation of studies with that species. For chemical restraint during minor procedures, diazepam, at a dose of 5.5-8.5 mg/kg im, has become the drug of choice. For surgical anesthesia, optimal results have been obtained using thiamylal sodium iv for induction of anesthesia and maintained by 0.5-1.5% halothane administered via an endotracheal cannula. Pentobarbital sodium may be used to produce surgical anesthesia but is generally unsatisfactory except for terminal procedures. Electroanesthesia, even with concomitant use of tranquilizing drugs, is difficult to control and results in very poor muscle relaxation.", "contents": "Restraint, venipuncture, endotracheal intubation, and anesthesia of miniature swine. Miniature swine are useful as experimental animals for investigations involving major surgical procedures, provided that proper restraint and anesthetic methods are employed. The methods and drugs described herein are not intended to be all-inclusive but rather those found most applicable to a wide variety of studies with miniature swine at this laboratory. This report is intended primarily to guide those unfamiliar in handling miniature swine during initiation of studies with that species. For chemical restraint during minor procedures, diazepam, at a dose of 5.5-8.5 mg/kg im, has become the drug of choice. For surgical anesthesia, optimal results have been obtained using thiamylal sodium iv for induction of anesthesia and maintained by 0.5-1.5% halothane administered via an endotracheal cannula. Pentobarbital sodium may be used to produce surgical anesthesia but is generally unsatisfactory except for terminal procedures. Electroanesthesia, even with concomitant use of tranquilizing drugs, is difficult to control and results in very poor muscle relaxation."}
{"id": "PMID:1097824", "title": "Experimental transmission of atypical ileal hyperplasia of hamsters.", "content": "Conditions for oral transmission of atypical ileal hyperplasia (AIH) in weanling hamsters were established and 22 passages were made. AIH was transmitted by feeding whole cell-free supernatants of ileal homogenates. The etiologic agent(s) was retained by 0.22 mum pore-size filters and was inactivated by chloroform treatment or by heating at 56 degrees C for 30 min. Enteric bacteria from affected animals also induced AIH, but with a lower morbidity and mortality than following inoculation with ileal extracts. Experimentally induced lesions progressed from marked segmental hyperplasia of ileal mucosa to granulomatous inflammation in underlying connective tissue and muscle tunics. Hyperplastic mucosal epithelium penetrated the muscularis mucosa, but metastases were not detected. Serum antibody from exposed animals reacted specifically, by indirect immunofluorescence, with an intracytoplasmic mucosal cell antigen(s) of autologous and homogolous ileal lesions, but antibody did not react with normal ileal mucosa or with unaffected portions of intestine from animals bearing ileal lesions.", "contents": "Experimental transmission of atypical ileal hyperplasia of hamsters. Conditions for oral transmission of atypical ileal hyperplasia (AIH) in weanling hamsters were established and 22 passages were made. AIH was transmitted by feeding whole cell-free supernatants of ileal homogenates. The etiologic agent(s) was retained by 0.22 mum pore-size filters and was inactivated by chloroform treatment or by heating at 56 degrees C for 30 min. Enteric bacteria from affected animals also induced AIH, but with a lower morbidity and mortality than following inoculation with ileal extracts. Experimentally induced lesions progressed from marked segmental hyperplasia of ileal mucosa to granulomatous inflammation in underlying connective tissue and muscle tunics. Hyperplastic mucosal epithelium penetrated the muscularis mucosa, but metastases were not detected. Serum antibody from exposed animals reacted specifically, by indirect immunofluorescence, with an intracytoplasmic mucosal cell antigen(s) of autologous and homogolous ileal lesions, but antibody did not react with normal ileal mucosa or with unaffected portions of intestine from animals bearing ileal lesions."}
{"id": "PMID:1097825", "title": "Parabiotic reinnervation: surgical technic and animal care.", "content": "A technic called parabiotic reinnervation was developed to test the hypothesis that a derangement of the trophic function normally exerted by a nerve on a muscle is an element in the pathogenesis of muscular dystrophy in the 129/ReJ dydy mouse. The technic involves the reinnervation of the muscles of the anterior compartment of the hind limb of 1 animal (the recipient) by the peroneal nerve of another animal (the donor). Survival of the parabiosed animals depends on attention to nutrition and cage environment.", "contents": "Parabiotic reinnervation: surgical technic and animal care. A technic called parabiotic reinnervation was developed to test the hypothesis that a derangement of the trophic function normally exerted by a nerve on a muscle is an element in the pathogenesis of muscular dystrophy in the 129/ReJ dydy mouse. The technic involves the reinnervation of the muscles of the anterior compartment of the hind limb of 1 animal (the recipient) by the peroneal nerve of another animal (the donor). Survival of the parabiosed animals depends on attention to nutrition and cage environment."}
{"id": "PMID:1097826", "title": "A rapid method for skin grafting in germfree rats.", "content": "A rapid method for skin grafting in germfree rats was developed. Six mm grafts are punched out on the tails of host and donor rats, grafts exchanged, oriented, and bound in place with non-allergic tape. Autografts healed within 5 da; first-set allografts between strains were usually rejected within 10 da. This technic provides a simple method for the assessment of skin graft responses in the germfree environment.", "contents": "A rapid method for skin grafting in germfree rats. A rapid method for skin grafting in germfree rats was developed. Six mm grafts are punched out on the tails of host and donor rats, grafts exchanged, oriented, and bound in place with non-allergic tape. Autografts healed within 5 da; first-set allografts between strains were usually rejected within 10 da. This technic provides a simple method for the assessment of skin graft responses in the germfree environment."}
{"id": "PMID:1097827", "title": "Basophilic degeneration of myocardium: an ultrastructural study.", "content": "The basophilic degeneration of myocardial cells is frequently observed in pathology. We studied the ultrastructure of the degeneration in human myocardial samples.  Thelesion varied in different regions that could be grouped in three various types.  One ofthe regions seems to be the point of origin of the degeneration; mitochondrial streaks, elements of the smooth or rough sarcoplasmic reticulum, and polyribisomes are found init.  All of the  structures are surrounded by a substance rich in fine filaments and microvesticles.  The other two regions are more compact; one is composed mainly of osmiophilic flakes and the other consists almost exclusively of filaments, some of them showinga periodicity of 200 A. The affected cells seem to be suffering from abnormal glycogen metabolism.  The substance found in basophilic degeneration shows a stron similarityto the corpora amylacea and the Lafora bodies.  This substance is probably a glycoprotein and seems to accumulate because of a betabolic disturbance.", "contents": "Basophilic degeneration of myocardium: an ultrastructural study. The basophilic degeneration of myocardial cells is frequently observed in pathology. We studied the ultrastructure of the degeneration in human myocardial samples.  Thelesion varied in different regions that could be grouped in three various types.  One ofthe regions seems to be the point of origin of the degeneration; mitochondrial streaks, elements of the smooth or rough sarcoplasmic reticulum, and polyribisomes are found init.  All of the  structures are surrounded by a substance rich in fine filaments and microvesticles.  The other two regions are more compact; one is composed mainly of osmiophilic flakes and the other consists almost exclusively of filaments, some of them showinga periodicity of 200 A. The affected cells seem to be suffering from abnormal glycogen metabolism.  The substance found in basophilic degeneration shows a stron similarityto the corpora amylacea and the Lafora bodies.  This substance is probably a glycoprotein and seems to accumulate because of a betabolic disturbance."}
{"id": "PMID:1097830", "title": "Considerations appropriate to a clinical trial of definitive radiation therapy in adenocarcinoma of the pancreas.", "content": "The Author describes criteria for selection of patients who might be eligible for entry into a clinical trial of definitive Radiation Therapy for adenocarcinoma of the pancreas. Most emphasis is placed on careful surgical staging, target volume and location, and the use of bypass procedures when appropriate.", "contents": "Considerations appropriate to a clinical trial of definitive radiation therapy in adenocarcinoma of the pancreas. The Author describes criteria for selection of patients who might be eligible for entry into a clinical trial of definitive Radiation Therapy for adenocarcinoma of the pancreas. Most emphasis is placed on careful surgical staging, target volume and location, and the use of bypass procedures when appropriate."}
{"id": "PMID:1097831", "title": "Prospectives for cell and organ culture systems in the study of pancreatic carcinoma.", "content": "Adenocarcinoma of the pancreas is presumed to arise from ductal cell precursors. This brief article questions the definitions on which this assumption is based and suggests possible ways to answer the question since for future studies on the etiology of this cancer it is imperative to study the appropriate cell of origin. Possible approaches for future work on pancreatic carcinogenesis utilizing cell and organ culture systems are discussed.", "contents": "Prospectives for cell and organ culture systems in the study of pancreatic carcinoma. Adenocarcinoma of the pancreas is presumed to arise from ductal cell precursors. This brief article questions the definitions on which this assumption is based and suggests possible ways to answer the question since for future studies on the etiology of this cancer it is imperative to study the appropriate cell of origin. Possible approaches for future work on pancreatic carcinogenesis utilizing cell and organ culture systems are discussed."}
{"id": "PMID:1097833", "title": "Palatal reconstruction utilizing retrieved forehead flap.", "content": "Ablation of tumors involving the tonsil and retromolar trigone complex often require partial resection of the soft palate. The pharyngeal wall defect is well reconstructed with the forehead flap. We have used the distal portion of the severed inserted forehead flap to reconstruct the soft palate defect successfully in 35 cases. The nasopharyngeal surface is lined with a book-flap from the remaining palate. This provides full palatal reconstruction with nasopharyngeal mucosa lining: thus preventing flap contracture and the unwanted sequelae of nasopharyngeal air and fluid reflux.", "contents": "Palatal reconstruction utilizing retrieved forehead flap. Ablation of tumors involving the tonsil and retromolar trigone complex often require partial resection of the soft palate. The pharyngeal wall defect is well reconstructed with the forehead flap. We have used the distal portion of the severed inserted forehead flap to reconstruct the soft palate defect successfully in 35 cases. The nasopharyngeal surface is lined with a book-flap from the remaining palate. This provides full palatal reconstruction with nasopharyngeal mucosa lining: thus preventing flap contracture and the unwanted sequelae of nasopharyngeal air and fluid reflux."}
{"id": "PMID:1097834", "title": "Cervical-pectoral flaps in the treatment of advanced oral cancer.", "content": "Twenty-two of 90 patients with carcinoma of the anterior oral cavity treated at the Minneapolis Veterans Administration Hospital from 1965 through 1973 had advanced lesions - tumors attached to the mandible and/or more than 3 cm in size. These patients were treated by primary radical neck dissection, en bloc excision, and segmental mandibulectomy. A cervical-pectoral flap combination was used for reconstruction. The functional and cosmetic results were excellent. Patient survival and cure rate compare favorably with other series. We emphasize the need for wide initial resection with immediate reconstruction.", "contents": "Cervical-pectoral flaps in the treatment of advanced oral cancer. Twenty-two of 90 patients with carcinoma of the anterior oral cavity treated at the Minneapolis Veterans Administration Hospital from 1965 through 1973 had advanced lesions - tumors attached to the mandible and/or more than 3 cm in size. These patients were treated by primary radical neck dissection, en bloc excision, and segmental mandibulectomy. A cervical-pectoral flap combination was used for reconstruction. The functional and cosmetic results were excellent. Patient survival and cure rate compare favorably with other series. We emphasize the need for wide initial resection with immediate reconstruction."}
{"id": "PMID:1097835", "title": "Appendectomy, tonsillectomy, and neoplasia.", "content": "Reports in the literature that study the frequency of cancer in individual who have had their tonsils or appendix or both removed are reviewed. Some investigators found a high incidence of solid cancers - in particular, breast and colon neoplasms - in female patients who have had appendectomy, but others disagreed. The association of tonsillectomy and/or appendectomy with the later development of malignant lymphomas is not proven.", "contents": "Appendectomy, tonsillectomy, and neoplasia. Reports in the literature that study the frequency of cancer in individual who have had their tonsils or appendix or both removed are reviewed. Some investigators found a high incidence of solid cancers - in particular, breast and colon neoplasms - in female patients who have had appendectomy, but others disagreed. The association of tonsillectomy and/or appendectomy with the later development of malignant lymphomas is not proven."}
{"id": "PMID:1097836", "title": "Cardiac function early after repair of tetralogy of Fallot.", "content": "Hemodynamics of 12 patients with tetralogy of Fallot were monitored during the first 72 hours after surgical repair. Total immediate repair in 5 patients was followed after 24 hours by a greater decrease in cardiac index than that observed in the group of 4 patients with previous palliative shunt (minus 25 plus or minus 6 vs. minus 1 plus or minus 7 per cent, p smaller than 0.025). This difference disappeared after 48 hours, and the short-term follow-up periods of these two groups were equally smooth. Six patients with pulmonary stenosis requiring the placement of an outflow patch had higher right ventricular filling pressures (after 24 hours 13.8 vs. 10.8 mm. Hg, p smaller than 0.025; 2 to 4 weeks later 9.6 vs. 5.5 mm. Hg, p smaller than 0.05), suggestive of a persistent right ventricular depression. This ventricular depression must be attributed to the induced pulmonary insufficiency and to the presence of akinetic areas. Both these factors should therefore be carefully minimized during the surgical procedure.", "contents": "Cardiac function early after repair of tetralogy of Fallot. Hemodynamics of 12 patients with tetralogy of Fallot were monitored during the first 72 hours after surgical repair. Total immediate repair in 5 patients was followed after 24 hours by a greater decrease in cardiac index than that observed in the group of 4 patients with previous palliative shunt (minus 25 plus or minus 6 vs. minus 1 plus or minus 7 per cent, p smaller than 0.025). This difference disappeared after 48 hours, and the short-term follow-up periods of these two groups were equally smooth. Six patients with pulmonary stenosis requiring the placement of an outflow patch had higher right ventricular filling pressures (after 24 hours 13.8 vs. 10.8 mm. Hg, p smaller than 0.025; 2 to 4 weeks later 9.6 vs. 5.5 mm. Hg, p smaller than 0.05), suggestive of a persistent right ventricular depression. This ventricular depression must be attributed to the induced pulmonary insufficiency and to the presence of akinetic areas. Both these factors should therefore be carefully minimized during the surgical procedure."}
{"id": "PMID:1097839", "title": "Consturction of new right ventricle on the epicardium: a possible correction for underdevelopment of the right ventricle.", "content": "By covering almost the entire right ventricular free wall and the anterior wall of the left ventricle with a Teflon cloth, we constructed a new chamber on the epicardium of a canine heart. An aortic homograft with the aortic valve was anastomosed to the right atrium as an inlet of this chamber. Another aortic homograft was sutured to the main pulmonary artery as an outlet. Pressure produced in this chamber ranged 25 to 55 mm. Hg and its first derivative was 450 to 1,870 mm. Hg per second. Thus this chamber had an excellent capability of epicardium will yield more physiological benefits to patients with an agenetic or hypoplastic right ventricle than do the current surgical procedures, in which a conduit is interposed between the right atrium and the main pulmonary artery. The procedures proposed could pressurize the pulmonary circulation and produce a pulsatile flow.", "contents": "Consturction of new right ventricle on the epicardium: a possible correction for underdevelopment of the right ventricle. By covering almost the entire right ventricular free wall and the anterior wall of the left ventricle with a Teflon cloth, we constructed a new chamber on the epicardium of a canine heart. An aortic homograft with the aortic valve was anastomosed to the right atrium as an inlet of this chamber. Another aortic homograft was sutured to the main pulmonary artery as an outlet. Pressure produced in this chamber ranged 25 to 55 mm. Hg and its first derivative was 450 to 1,870 mm. Hg per second. Thus this chamber had an excellent capability of epicardium will yield more physiological benefits to patients with an agenetic or hypoplastic right ventricle than do the current surgical procedures, in which a conduit is interposed between the right atrium and the main pulmonary artery. The procedures proposed could pressurize the pulmonary circulation and produce a pulsatile flow."}
{"id": "PMID:1097840", "title": "Platelet support for cardiopulmonary bypass surgery.", "content": "After we discontinued the use of fresh blood for cardiopulmonary bypass surgery, we routinely provided platelet concentrates for the patients. To ascertain if this was necessary, patients paired for procedure and age were given either 4 units of platelets (Group I) or no platelets (Group II). Platelet counts were obtained preoperatively; hourly during bypass; immediately, 1/2 hour, and 3 to 4 hours after bypass; and daily for 7 postoperative days. In the 60 patients evaluated, a significant difference between mean platelet counts could not be demonstrated at any time. Total blood use and the total time required for postbypass hemostasis was not significantly different between the two groups. The degree of thrombocytopenia could not be correlated by bypass time. Platelet concentrates or fresh blood are not needed prophylactically for cardiopulmonary bypass surgery, and their usage should be reserved for the occasional patient who manifests thrombocytopenia as well as hemorrhagic complications.", "contents": "Platelet support for cardiopulmonary bypass surgery. After we discontinued the use of fresh blood for cardiopulmonary bypass surgery, we routinely provided platelet concentrates for the patients. To ascertain if this was necessary, patients paired for procedure and age were given either 4 units of platelets (Group I) or no platelets (Group II). Platelet counts were obtained preoperatively; hourly during bypass; immediately, 1/2 hour, and 3 to 4 hours after bypass; and daily for 7 postoperative days. In the 60 patients evaluated, a significant difference between mean platelet counts could not be demonstrated at any time. Total blood use and the total time required for postbypass hemostasis was not significantly different between the two groups. The degree of thrombocytopenia could not be correlated by bypass time. Platelet concentrates or fresh blood are not needed prophylactically for cardiopulmonary bypass surgery, and their usage should be reserved for the occasional patient who manifests thrombocytopenia as well as hemorrhagic complications."}
{"id": "PMID:1097841", "title": "Morphologic observations on heart valve prostheses made of fascia lata.", "content": "A cooperative, international study permitted morphologic observations on 58 fascia lata heart valve prosthese. Thirty-nine had been in position less than a year, the remainder for periods up to 50 months. The fascial cusps, no matter whether free or stent mounted, underwent changes that included death of the original fascia and vacuolar, degenerative changes if a valve had been in situ more than 3 years. Formation of a pseudointima on a cusp's surface caused it to shrink. The rate of progression and severity of the changes varied from prosthesis to prosthesis. Severe cusp shrinkage caused valve incompetence. That seemed particularly likely in mitral and tricuspid prostheses and explains why their use has been abandoned. Clinically, fascia lata aortic valves function satisfactorily for several years. However, their long-term value is still uncertain. Morphologically, the specter of late calcification and other degenerative changes haunt their use until more, from long-term survivors, are available for study.", "contents": "Morphologic observations on heart valve prostheses made of fascia lata. A cooperative, international study permitted morphologic observations on 58 fascia lata heart valve prosthese. Thirty-nine had been in position less than a year, the remainder for periods up to 50 months. The fascial cusps, no matter whether free or stent mounted, underwent changes that included death of the original fascia and vacuolar, degenerative changes if a valve had been in situ more than 3 years. Formation of a pseudointima on a cusp's surface caused it to shrink. The rate of progression and severity of the changes varied from prosthesis to prosthesis. Severe cusp shrinkage caused valve incompetence. That seemed particularly likely in mitral and tricuspid prostheses and explains why their use has been abandoned. Clinically, fascia lata aortic valves function satisfactorily for several years. However, their long-term value is still uncertain. Morphologically, the specter of late calcification and other degenerative changes haunt their use until more, from long-term survivors, are available for study."}
{"id": "PMID:1097842", "title": "Lymphocele: a significant complication following renal transplantation.", "content": "Six pelvic lymphoceles occurred in a series of 88 renal transplants. All of the patients had ipsilateral leg edema and one-half had a urinary tract infection and/or pain. Displacement of the urinary bladder away from the kidney with or without some degree of ureteral obstruction was diagnostic. External or internal drainage resulted in reliev of the symptoms.", "contents": "Lymphocele: a significant complication following renal transplantation. Six pelvic lymphoceles occurred in a series of 88 renal transplants. All of the patients had ipsilateral leg edema and one-half had a urinary tract infection and/or pain. Displacement of the urinary bladder away from the kidney with or without some degree of ureteral obstruction was diagnostic. External or internal drainage resulted in reliev of the symptoms."}
{"id": "PMID:1097843", "title": "Isiopathic postpartum renal failure: review and case report of a successful renal transplantation.", "content": "Idiopathic postpartum renal failure is a syndrome of acute, rapidly progressive oliguria, azotemia, and microangiopathic hemolytic anemia occurring shortly after a normal pregnancy and usually terminating fatally.  Of 25 patients described in the literature, 8 survived: 1 recovered almost normal renal function, 4 had renal functional impairment, and 3 required maintenance hemodialysis.  This report concerns a patient who survived after nephrectomy, hemodialysis, and successful renal transplantation from a living related donor.  Theoretic and experimental studies indicate that heparin, platelet inhibitors, and fibrinolytic agents should be beneficial if given early.  If the disease progresses to the irreversible stage, nephrectomy and renal transplantation are warranted since the disorder does not seem to recur after transplantation.", "contents": "Isiopathic postpartum renal failure: review and case report of a successful renal transplantation. Idiopathic postpartum renal failure is a syndrome of acute, rapidly progressive oliguria, azotemia, and microangiopathic hemolytic anemia occurring shortly after a normal pregnancy and usually terminating fatally.  Of 25 patients described in the literature, 8 survived: 1 recovered almost normal renal function, 4 had renal functional impairment, and 3 required maintenance hemodialysis.  This report concerns a patient who survived after nephrectomy, hemodialysis, and successful renal transplantation from a living related donor.  Theoretic and experimental studies indicate that heparin, platelet inhibitors, and fibrinolytic agents should be beneficial if given early.  If the disease progresses to the irreversible stage, nephrectomy and renal transplantation are warranted since the disorder does not seem to recur after transplantation."}
{"id": "PMID:1097845", "title": "The viral theory of connective tissue diseases: a review.", "content": "For many years evidence for a viral aetiology of connective tissue diseases such as systemic lupus erythematosus and rheumatoid arthritis has been sought by a variety of methods, including attempts at direct isolation, the study of rheumatoid synovial cells, ultrastructural examination of pathological material and assays of anti-viral antibody. No convincing proof has yet been obtained. However, a better understanding of the mechanisms of viral persistence, and the ways in which host defences can be subverted by viral infections has prompted other ways of approaching this problem. A viral aetiology for this group of diseases remains an attractive but unsubstantiated hypothesis.", "contents": "The viral theory of connective tissue diseases: a review. For many years evidence for a viral aetiology of connective tissue diseases such as systemic lupus erythematosus and rheumatoid arthritis has been sought by a variety of methods, including attempts at direct isolation, the study of rheumatoid synovial cells, ultrastructural examination of pathological material and assays of anti-viral antibody. No convincing proof has yet been obtained. However, a better understanding of the mechanisms of viral persistence, and the ways in which host defences can be subverted by viral infections has prompted other ways of approaching this problem. A viral aetiology for this group of diseases remains an attractive but unsubstantiated hypothesis."}
{"id": "PMID:1097846", "title": "State response to federal policy: children, EPSDT, and the Medicaid Muddle.", "content": "The implementation in Connecticut of Early and Periodic Screening, Diagnosis and Treatment (EPSDT), a program of comprehensive medical care for needy children, illustrates the complexities engendered by federally mandated state-administered health programs.  The EPSDT amendments to Title V and Title XIX (Medicaid) were ambiguous on four major issues: administrative responsibility, costs, eligibility, and scope of services.  The problems experienced by federal, regional, state, and local administrators in resolving these issues illustrate the weaknesses inherent in federal-state relations, and the contrasting roles health and welfare agencies under Medicaid have played in the development of health policy.  Connecticut may represent maximum limits to state performance in carrying out EPSDT because it is a rich state, ranking high in medical resources and in the provision of Medicaid services.  During the first year of implementation of EPSDT, the program had little impact: less than 5 percent of eligible children were served.  State policies, which contravened federal policy, precluded effective resolution of the legislative ambiguities; no new services were added, the organization of health services remained unchanged and fragmented; and the State Health Department played only a limited role.", "contents": "State response to federal policy: children, EPSDT, and the Medicaid Muddle. The implementation in Connecticut of Early and Periodic Screening, Diagnosis and Treatment (EPSDT), a program of comprehensive medical care for needy children, illustrates the complexities engendered by federally mandated state-administered health programs.  The EPSDT amendments to Title V and Title XIX (Medicaid) were ambiguous on four major issues: administrative responsibility, costs, eligibility, and scope of services.  The problems experienced by federal, regional, state, and local administrators in resolving these issues illustrate the weaknesses inherent in federal-state relations, and the contrasting roles health and welfare agencies under Medicaid have played in the development of health policy.  Connecticut may represent maximum limits to state performance in carrying out EPSDT because it is a rich state, ranking high in medical resources and in the provision of Medicaid services.  During the first year of implementation of EPSDT, the program had little impact: less than 5 percent of eligible children were served.  State policies, which contravened federal policy, precluded effective resolution of the legislative ambiguities; no new services were added, the organization of health services remained unchanged and fragmented; and the State Health Department played only a limited role."}
{"id": "PMID:1097847", "title": "Cost-sharing and prior authorization effects on Medicaid services in California:  Part II: The providers' reactions.", "content": "Providers for California's Medical program were interviewed to determine their reaction to the copayment experiment.  Private physicians, pharmacists, and nursing homes routinely collected the copayments and though it was not a particular hardship on the patients.  Hospital outpatient departments often did not collect.  Physicians both in hospitals and in private practice generally did not know which patients were copayers and hence copayment had no effect on their practice.  Pharmacists reported some delay and some selectivity on the part of patients in the filling of their prescriptions.  All providers felt as much more harassment and interference from  the concurrent prior authorization requirements.  This effect may have swamped copayment effects.", "contents": "Cost-sharing and prior authorization effects on Medicaid services in California:  Part II: The providers' reactions. Providers for California's Medical program were interviewed to determine their reaction to the copayment experiment.  Private physicians, pharmacists, and nursing homes routinely collected the copayments and though it was not a particular hardship on the patients.  Hospital outpatient departments often did not collect.  Physicians both in hospitals and in private practice generally did not know which patients were copayers and hence copayment had no effect on their practice.  Pharmacists reported some delay and some selectivity on the part of patients in the filling of their prescriptions.  All providers felt as much more harassment and interference from  the concurrent prior authorization requirements.  This effect may have swamped copayment effects."}
{"id": "PMID:1097848", "title": "Risk vulnerability and enrollment in a prepaid group practice.", "content": "An hypothesis that specifies conditions under which risk vulnerability factors are associated with enrollment in prepaid practices is presented and tested.  We assert that such factors as illness histories and previous use of medical services are linearly and positively associated with enrollment when the prepaid option offers significantly lower marginal out-of-pocket costs.  The hypothesis is supported by observations from a prostpective study of a lowincome population.", "contents": "Risk vulnerability and enrollment in a prepaid group practice. An hypothesis that specifies conditions under which risk vulnerability factors are associated with enrollment in prepaid practices is presented and tested.  We assert that such factors as illness histories and previous use of medical services are linearly and positively associated with enrollment when the prepaid option offers significantly lower marginal out-of-pocket costs.  The hypothesis is supported by observations from a prostpective study of a lowincome population."}
{"id": "PMID:1097849", "title": "The potential for radiation sensitizers and possible strategy for use.", "content": "The search for radiation sensitzers for use in radiotherapy is based on the assumption that proper use of these compounds will provide a differential sensitization of tumor and normal tissue. Based on the evidence that hypoxic cells exist within human tumors but not within normal cell populations, there is reason to believe that specific sensitizers of hypoxic cells might provide useful therapeutic benefit. This paper discusses the possible advantages of such sensitizers, over reliance on the phenomenon of reoxygenation, and describes very briefly the current status of hypoxic cell sensitizers. Finally a proposal is made concerning the way in which such sensitizers might be tested for their efficacy in human radiotherapy.", "contents": "The potential for radiation sensitizers and possible strategy for use. The search for radiation sensitzers for use in radiotherapy is based on the assumption that proper use of these compounds will provide a differential sensitization of tumor and normal tissue. Based on the evidence that hypoxic cells exist within human tumors but not within normal cell populations, there is reason to believe that specific sensitizers of hypoxic cells might provide useful therapeutic benefit. This paper discusses the possible advantages of such sensitizers, over reliance on the phenomenon of reoxygenation, and describes very briefly the current status of hypoxic cell sensitizers. Finally a proposal is made concerning the way in which such sensitizers might be tested for their efficacy in human radiotherapy."}
{"id": "PMID:1097850", "title": "Nasal reconstruction.", "content": "Congenital malformations, trauma, benign or malignant tumors may call for reconstructive surgical procedures to cover the defect. In order to establish a proper diagnosis a careful examination including X-rays and biopsies is mandatory. A close contact with with the radiotherapist is most desirable in order to combine the therapy if necessary. After the excision of scars or growth, we differentiate between three phases of treatment: 1. The immediate reconstruction either by local flaps or by free grafts. 2. The delayed excision of the tumor after the establishment of the proper diagnosis. This waiting period (two to three weeks) is used to preform the missing nasal parts in the vicinity of the lesion either on the cheek or forehead. Rib cartilage with split skin grafts or composite grafts is used for this purpose. After the excision of the tumor the nasal reconstruction is carried out during the same operation. 3. Tumor excision and coverage of the raw surface with split grafts in order to inspect the area for several months for any recurrence and the delayed reconstruction with adjacent or distant flaps. The operative techniques are described.", "contents": "Nasal reconstruction. Congenital malformations, trauma, benign or malignant tumors may call for reconstructive surgical procedures to cover the defect. In order to establish a proper diagnosis a careful examination including X-rays and biopsies is mandatory. A close contact with with the radiotherapist is most desirable in order to combine the therapy if necessary. After the excision of scars or growth, we differentiate between three phases of treatment: 1. The immediate reconstruction either by local flaps or by free grafts. 2. The delayed excision of the tumor after the establishment of the proper diagnosis. This waiting period (two to three weeks) is used to preform the missing nasal parts in the vicinity of the lesion either on the cheek or forehead. Rib cartilage with split skin grafts or composite grafts is used for this purpose. After the excision of the tumor the nasal reconstruction is carried out during the same operation. 3. Tumor excision and coverage of the raw surface with split grafts in order to inspect the area for several months for any recurrence and the delayed reconstruction with adjacent or distant flaps. The operative techniques are described."}
{"id": "PMID:1097858", "title": "Diabetic retinopathy.", "content": "Retinopathy is present in 60%-80% of long-term diabetics, and 5%-10% of diabetics surviving 20 yr from the time of diagnosis will be blind, mostly from retinopathy, which is now the commonest cause of newly diagnosed blindness in the 30-65 yr age group. The mean survival time after a diagnosis of retinopathy is only 5 yr. The natural history of diabetic retinopathy is now being understood more clearly. Mild background retinopathy, characterised by microaneurysms or scattered hard exudates, may progress to maculopathy, with macular vascular pathology leading to exudates and edema at the macula; this is most common in older patients. It may also lead to proliferative retinopathy, which may progress slowly with new vessels and fibrous-tissue formation, or rapidly with widespread capillary closure and soft-exudate formation, extensive neovascularisation, hemorrhages, and blindness. The changes of diabetic retinopathy can be documented using retinal photography, and several grading methods have been devised that are useful for evaluation treatment. The cause of diabetic retinopathy is still unclear. Evidence for incriminating genetic factors, growth-hormone excess, and hypoxia associated with changes in blood flow and retinal metabolism are reviewed. Insulin responses and plasma triglyceride seem to be different in maturity-onset diabetics with retinopathy when compared with those in whom this complication is absent. Most physicians agree that good diabetic control may both lower the incidence of retinopathy and reduce the speed of its progress. While there is little evidence that drugs are ever of much value in this condition, the role of photocoagulation both by laser and xenon arc is becoming clearer with increased experience of these techniques, and the current situation is reviewed. Pituitary ablation is a very drastic method of treatment and should never be used as a desperate measure in a patient with advanced proliferative disease. It is, however, the treatment of choice in early florid retinopathy, when it proves the only chance to arrest the condition. Some new techniques of vitreous surgery are now being developed and the possible role of these in the management of patients with advanced proliferative disease is briefly reviewed.", "contents": "Diabetic retinopathy. Retinopathy is present in 60%-80% of long-term diabetics, and 5%-10% of diabetics surviving 20 yr from the time of diagnosis will be blind, mostly from retinopathy, which is now the commonest cause of newly diagnosed blindness in the 30-65 yr age group. The mean survival time after a diagnosis of retinopathy is only 5 yr. The natural history of diabetic retinopathy is now being understood more clearly. Mild background retinopathy, characterised by microaneurysms or scattered hard exudates, may progress to maculopathy, with macular vascular pathology leading to exudates and edema at the macula; this is most common in older patients. It may also lead to proliferative retinopathy, which may progress slowly with new vessels and fibrous-tissue formation, or rapidly with widespread capillary closure and soft-exudate formation, extensive neovascularisation, hemorrhages, and blindness. The changes of diabetic retinopathy can be documented using retinal photography, and several grading methods have been devised that are useful for evaluation treatment. The cause of diabetic retinopathy is still unclear. Evidence for incriminating genetic factors, growth-hormone excess, and hypoxia associated with changes in blood flow and retinal metabolism are reviewed. Insulin responses and plasma triglyceride seem to be different in maturity-onset diabetics with retinopathy when compared with those in whom this complication is absent. Most physicians agree that good diabetic control may both lower the incidence of retinopathy and reduce the speed of its progress. While there is little evidence that drugs are ever of much value in this condition, the role of photocoagulation both by laser and xenon arc is becoming clearer with increased experience of these techniques, and the current situation is reviewed. Pituitary ablation is a very drastic method of treatment and should never be used as a desperate measure in a patient with advanced proliferative disease. It is, however, the treatment of choice in early florid retinopathy, when it proves the only chance to arrest the condition. Some new techniques of vitreous surgery are now being developed and the possible role of these in the management of patients with advanced proliferative disease is briefly reviewed."}
{"id": "PMID:1097864", "title": "Topical treatment of recurrent herpes simplex with cytosine arabinoside.", "content": "A double-blind controlled clinical trial of the topical use of cytosine arabinoside in recurrent herpes simplex of the face and genital region showed no significant difference in the efficiency of a placebo as compared with cytosine arabinoside. The high rate of placebo response (70%) found in this trial makes the assessment of any drug treatment for herpes simplex particularly difficult.", "contents": "Topical treatment of recurrent herpes simplex with cytosine arabinoside. A double-blind controlled clinical trial of the topical use of cytosine arabinoside in recurrent herpes simplex of the face and genital region showed no significant difference in the efficiency of a placebo as compared with cytosine arabinoside. The high rate of placebo response (70%) found in this trial makes the assessment of any drug treatment for herpes simplex particularly difficult."}
{"id": "PMID:1097866", "title": "Effect of soya bean milk on the healing of gastric ulcers -- a controlled endoscopic study.", "content": "Soya bean milk was given orally in doses of 150 ml second-hourly (7 a.m. to 11 p.m. daily) to 10 Chinese subjects with proven gastric ulcer for two weeks. The control group consisted of 10 Chinese subjects with proven gastric ulcer who did not receive soya bean milk therapy. All were inpatients and were given bed rest. Gastric ulcer healing was assessed endoscopically with a duodenofibrescope. Endoscopic visualization and colour photography of the ulcer crater were undertaken just before and after two weeks of treatment. In the soya bean milk group, complete healing was seen in two cases, considerable healing in four cases, slight healing in two cases, and no healing in two cases. In the control group, complete healing was seen in no cases, considerably healing in three cases, slight healing in four cases, and no healing in three cases. The mean ulcer healing grade was 1.600 in the soya bean milk group as compared with 1.000 in the controls. The difference was not statistically significant. There was no significant difference between the two groups in the initial ulcer size, age and sex distribution. Although the effect on gastric ulcer healing was not significant, soya bean milk has been shown to be effective in the relief of peptic ulcer pain.", "contents": "Effect of soya bean milk on the healing of gastric ulcers -- a controlled endoscopic study. Soya bean milk was given orally in doses of 150 ml second-hourly (7 a.m. to 11 p.m. daily) to 10 Chinese subjects with proven gastric ulcer for two weeks. The control group consisted of 10 Chinese subjects with proven gastric ulcer who did not receive soya bean milk therapy. All were inpatients and were given bed rest. Gastric ulcer healing was assessed endoscopically with a duodenofibrescope. Endoscopic visualization and colour photography of the ulcer crater were undertaken just before and after two weeks of treatment. In the soya bean milk group, complete healing was seen in two cases, considerable healing in four cases, slight healing in two cases, and no healing in two cases. In the control group, complete healing was seen in no cases, considerably healing in three cases, slight healing in four cases, and no healing in three cases. The mean ulcer healing grade was 1.600 in the soya bean milk group as compared with 1.000 in the controls. The difference was not statistically significant. There was no significant difference between the two groups in the initial ulcer size, age and sex distribution. Although the effect on gastric ulcer healing was not significant, soya bean milk has been shown to be effective in the relief of peptic ulcer pain."}
{"id": "PMID:1097869", "title": "A controlled study of a tetracyclic antidepressant--maprotiline (Ludiomil).", "content": "Maprotiline (Ludiomil) is a dibenzo bicyclooctadiene pharmacologically related to the tricyclic antidepressants and stereochemically having a tetracyclic structure. A controlled, double-blind comparison of maprotiline and imipramine in a group of 71 female outpatients with primary depression is presented. Maprotiline was found to be at least as effective as imipramine and significantly better in depressive neurosis. It also showed a bipolar action, being effective in both agitation and retardation.", "contents": "A controlled study of a tetracyclic antidepressant--maprotiline (Ludiomil). Maprotiline (Ludiomil) is a dibenzo bicyclooctadiene pharmacologically related to the tricyclic antidepressants and stereochemically having a tetracyclic structure. A controlled, double-blind comparison of maprotiline and imipramine in a group of 71 female outpatients with primary depression is presented. Maprotiline was found to be at least as effective as imipramine and significantly better in depressive neurosis. It also showed a bipolar action, being effective in both agitation and retardation."}
{"id": "PMID:1097875", "title": "[Acute leukemia in the terminal phase of Hodgkin's disease: a consequence of the depressed immune function?].", "content": "A 52 year old patient suffering from Hodgkin's disease received ionizing radiation and chemotherapy. 7 years after the diagnosis was made he died of acute leukemia. In the recent past similar cases were reported in the literature. There it was suggested that the leukemogenic effect of treatment may be an etiological factor for the development of leukemia. The clinical and experimental data show that the immune function of thymus-dependent lymphocytes is depressed in patients with Hodgkin's disease. According to the Burnet's immune surveillance theory the T-lymphocytes eliminate malignant cells in an early stage. If this T-dependent immune function is depressed - as in Hodgkin's disease - further malignancies may develop.", "contents": "[Acute leukemia in the terminal phase of Hodgkin's disease: a consequence of the depressed immune function?]. A 52 year old patient suffering from Hodgkin's disease received ionizing radiation and chemotherapy. 7 years after the diagnosis was made he died of acute leukemia. In the recent past similar cases were reported in the literature. There it was suggested that the leukemogenic effect of treatment may be an etiological factor for the development of leukemia. The clinical and experimental data show that the immune function of thymus-dependent lymphocytes is depressed in patients with Hodgkin's disease. According to the Burnet's immune surveillance theory the T-lymphocytes eliminate malignant cells in an early stage. If this T-dependent immune function is depressed - as in Hodgkin's disease - further malignancies may develop."}
{"id": "PMID:1097876", "title": "[Monocytic leukemias with unusual clinical presentations].", "content": "In clinical hematology the terms \"monocytic\" leukemia and \"reticulosis\" still -require better definition and classification. By presenting the histories of eight patients and by cytology and cytochemistry it is shown that myelo-monocytic leukemias can have the course well-known for an acute leukemia, including different skin lesions, as well as that of an typical chronic granulocytic leukemia. In dermatology monocytic leukemias were considered as belonging to the entity of the so-called reticulosarcomatosis cutis. However, strict differentiation from the hiary cell leukemia is to be made today. The general term \"reticuloses\" has quite faulty been used formerly for classification of these last two disorders.", "contents": "[Monocytic leukemias with unusual clinical presentations]. In clinical hematology the terms \"monocytic\" leukemia and \"reticulosis\" still -require better definition and classification. By presenting the histories of eight patients and by cytology and cytochemistry it is shown that myelo-monocytic leukemias can have the course well-known for an acute leukemia, including different skin lesions, as well as that of an typical chronic granulocytic leukemia. In dermatology monocytic leukemias were considered as belonging to the entity of the so-called reticulosarcomatosis cutis. However, strict differentiation from the hiary cell leukemia is to be made today. The general term \"reticuloses\" has quite faulty been used formerly for classification of these last two disorders."}
{"id": "PMID:1097878", "title": "[Testing of the bronchodilator NAB 365 in a double blind trial author's transl].", "content": "In a double blind trial NAD 365 (3 X 10 and 20 mug), Salbutamol (3 X 2,0 mg), and Terbutalin (3 X 2,5 mg) were compared for 14 days. In a total of 96 patients five parameters concerning function were tested-p02, pC02, airway resistance (Rt), intrathoracic gas volume (IGV), and forced expiratory flow. Although the doses for NAB 365 were considerably lower than those of the comparative substances, the degree of efficacy for NAB 365, Salbutamol and Terbutalin was the same. Adverse reactions of all three substances under consideration were negligible. The higher dose of NAB 365 showed the least favoralbe result in this respect.", "contents": "[Testing of the bronchodilator NAB 365 in a double blind trial author's transl]. In a double blind trial NAD 365 (3 X 10 and 20 mug), Salbutamol (3 X 2,0 mg), and Terbutalin (3 X 2,5 mg) were compared for 14 days. In a total of 96 patients five parameters concerning function were tested-p02, pC02, airway resistance (Rt), intrathoracic gas volume (IGV), and forced expiratory flow. Although the doses for NAB 365 were considerably lower than those of the comparative substances, the degree of efficacy for NAB 365, Salbutamol and Terbutalin was the same. Adverse reactions of all three substances under consideration were negligible. The higher dose of NAB 365 showed the least favoralbe result in this respect."}
{"id": "PMID:1097881", "title": "[Isolation of Yersinia enterocolitica in acute ileitis].", "content": "2246 stool specimens were specifically testet for Yersinia enterocolitica during 1973/74. In one case it was possible to isolate a strain of Yersinia enterocolitica (0-group I of Knapp/Serotype 3 of Winblad) from the feces of a patient suffering of acute ileitis. A high agglutinine-titer was serologically detected against a bacterial suspension of the isolated strain as well as of a reference strain (Serotype 3 of Winblad). The antibody-titer was 1 : 3200 by the first examination and dropped to 1 : 160 after two months. A reisolation attempt was unsuccessful.", "contents": "[Isolation of Yersinia enterocolitica in acute ileitis]. 2246 stool specimens were specifically testet for Yersinia enterocolitica during 1973/74. In one case it was possible to isolate a strain of Yersinia enterocolitica (0-group I of Knapp/Serotype 3 of Winblad) from the feces of a patient suffering of acute ileitis. A high agglutinine-titer was serologically detected against a bacterial suspension of the isolated strain as well as of a reference strain (Serotype 3 of Winblad). The antibody-titer was 1 : 3200 by the first examination and dropped to 1 : 160 after two months. A reisolation attempt was unsuccessful."}
{"id": "PMID:1097888", "title": "Accumulation of replicating bacterial plasmid DNA during thymine limitation or hydroxyurea treatment.", "content": "The rate of DNA chain elongation of a thymine auxotroph of Proteus mirabilis harboring an R factor was reduced by either thymine limitation or treatment with hydroxyurea.  Using CsCl density gradient centrifugation, sedimentation in sucrose gradients, and electron microscopy, it has been shown that the fraction of total R factor DNA which is in the process of replication is greatly increased when the rate of DNA chain elongation is reduced while the initiation of plasmid replication continues at the normal rate.  This makes possible the isolation and characterization of replicating plasmid DNA.  Electron microscopy revealed that replicating R factor DNA consists of double-branched (theta type) circular molecules.", "contents": "Accumulation of replicating bacterial plasmid DNA during thymine limitation or hydroxyurea treatment. The rate of DNA chain elongation of a thymine auxotroph of Proteus mirabilis harboring an R factor was reduced by either thymine limitation or treatment with hydroxyurea.  Using CsCl density gradient centrifugation, sedimentation in sucrose gradients, and electron microscopy, it has been shown that the fraction of total R factor DNA which is in the process of replication is greatly increased when the rate of DNA chain elongation is reduced while the initiation of plasmid replication continues at the normal rate.  This makes possible the isolation and characterization of replicating plasmid DNA.  Electron microscopy revealed that replicating R factor DNA consists of double-branched (theta type) circular molecules."}
{"id": "PMID:1097889", "title": "Non-enzymatic translocation in ribosomes from streptomycin-resistant mutants of Escherichia coli.", "content": "The capablity of ribosomes of four types of streptomycin-resistant mutants (A1, A2, A40 and A60) for non-enzymatic (EF-G--GTP-independent) translocation was tested.  It was found that an A40 type mutation (amino acid replacement in position 87 of the protein S12 polypeptide chain) leads to activation of the capablity of the ribosome to perform spontaneous non-enzymatic translocation, while type A1, A2 and A60 mutations (amino acid replacements in position 42 of protein S12) does not give such an effect.  Thus, it is shown that non-enzymatic translocation can be activated not only by the earlier described damage of the protein S12 by para-chloromercuribenzoate or by the complete removal of protein S12, but also by a definate mutational alteration of the protein.  Preliminary data are also reported on the possibility of activating non-enzymatic translocation by combinations of mutational alterations of the ribosomal proteins other than protein S12 but interdepending with it (such as S4 and S5).", "contents": "Non-enzymatic translocation in ribosomes from streptomycin-resistant mutants of Escherichia coli. The capablity of ribosomes of four types of streptomycin-resistant mutants (A1, A2, A40 and A60) for non-enzymatic (EF-G--GTP-independent) translocation was tested.  It was found that an A40 type mutation (amino acid replacement in position 87 of the protein S12 polypeptide chain) leads to activation of the capablity of the ribosome to perform spontaneous non-enzymatic translocation, while type A1, A2 and A60 mutations (amino acid replacements in position 42 of protein S12) does not give such an effect.  Thus, it is shown that non-enzymatic translocation can be activated not only by the earlier described damage of the protein S12 by para-chloromercuribenzoate or by the complete removal of protein S12, but also by a definate mutational alteration of the protein.  Preliminary data are also reported on the possibility of activating non-enzymatic translocation by combinations of mutational alterations of the ribosomal proteins other than protein S12 but interdepending with it (such as S4 and S5)."}
{"id": "PMID:1097890", "title": "Conjugational behaviour of N plasmids in Escherichia coli K12.", "content": "R factors of the N incompatibility group apparently transfer at low frequencies (often 10(-6)-10(-5) per donor cell) in 30 min liquid matings.  However, the level of transfer is greatly increased when donor-recipient mixtures are held on solid medium selective for the recipient only, prior to transconjugant selection.  Increase in transconjugant recovery is still donor-dependent at plating and therefore arises from mating on the plate.  The process limiting N-mediated conjugation in liquid is probably mating pair formation, suggesting that the increase in yield of transconjugants may result from provision of a solid substrate for mating pair formation rather than from the delay in selection per se.  The kinetics of N plasmid transfer on solid medium resemble those of traniments suggest that RP4, the prototype P incompatibility group plasmid, shows a stimulation of transfer on solid medium similar to the N plasmids studied.  We suggest that N, and probably P, plasmids are (like F and the col V's) naturally derepressed for fertility, but this is masked by the imcompetence in liquid matings of donors carrying them.", "contents": "Conjugational behaviour of N plasmids in Escherichia coli K12. R factors of the N incompatibility group apparently transfer at low frequencies (often 10(-6)-10(-5) per donor cell) in 30 min liquid matings.  However, the level of transfer is greatly increased when donor-recipient mixtures are held on solid medium selective for the recipient only, prior to transconjugant selection.  Increase in transconjugant recovery is still donor-dependent at plating and therefore arises from mating on the plate.  The process limiting N-mediated conjugation in liquid is probably mating pair formation, suggesting that the increase in yield of transconjugants may result from provision of a solid substrate for mating pair formation rather than from the delay in selection per se.  The kinetics of N plasmid transfer on solid medium resemble those of traniments suggest that RP4, the prototype P incompatibility group plasmid, shows a stimulation of transfer on solid medium similar to the N plasmids studied.  We suggest that N, and probably P, plasmids are (like F and the col V's) naturally derepressed for fertility, but this is masked by the imcompetence in liquid matings of donors carrying them."}
{"id": "PMID:1097891", "title": "Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells.", "content": "The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to assume that all the cells are competent). About 75% of this DNA was found to be attached to the cytoplasmic membrane. Employing Poisson approximation for distribution of the donor-marker DNA molecules on recipient cells it was found that the efficiency of a marker recombination and expression is about 2-10(-5). The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor 0exogenous", "contents": "Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells. The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to assume that all the cells are competent). About 75% of this DNA was found to be attached to the cytoplasmic membrane. Employing Poisson approximation for distribution of the donor-marker DNA molecules on recipient cells it was found that the efficiency of a marker recombination and expression is about 2-10(-5). The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor 0exogenous"}
{"id": "PMID:1097892", "title": "A method for isolating nonsense suppressors in enterobacteriaceae using an amber mutant of the drug resistance factor R1.", "content": "We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins.  We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that contain nonsense suppressors (Sup- strains) by screening penicillin-resistant revertants of strains containing the mutant R factor for the presence of such suppressors.  This obviates the need to have known nonsense mutations in chromosomal genes.  Theoretically, suppressor-containing derivatives of any bacterial species that can maintain and express R1 can be constructed.", "contents": "A method for isolating nonsense suppressors in enterobacteriaceae using an amber mutant of the drug resistance factor R1. We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins.  We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that contain nonsense suppressors (Sup- strains) by screening penicillin-resistant revertants of strains containing the mutant R factor for the presence of such suppressors.  This obviates the need to have known nonsense mutations in chromosomal genes.  Theoretically, suppressor-containing derivatives of any bacterial species that can maintain and express R1 can be constructed."}
{"id": "PMID:1097893", "title": "[Continuous positive airway pressure in the spontaneously breathing newborn by means of bilateral nasal cannulation(author's transl)].", "content": "Continuous positive airway pressure (CPAP) by means of a bilateral nasal cannula system has been used in 21 newborns.  15 had hyaline membrane disease, 3 had an aspiration and 3 showed extensive atelectasis.  In 18 of the 21 patients, paO2 increased after cannulation, FiO2 being kept unaltered.  As a consequence, the oxygen concentration in the inspired gas mixture could be lowered.  3 children had to be intubated endotracheally and ventilated artificially despite previous nasal cannulation.  The criteria for using the CPAP and the methodology are described, its advantages, disadvantages and dangers discussed.", "contents": "[Continuous positive airway pressure in the spontaneously breathing newborn by means of bilateral nasal cannulation(author's transl)]. Continuous positive airway pressure (CPAP) by means of a bilateral nasal cannula system has been used in 21 newborns.  15 had hyaline membrane disease, 3 had an aspiration and 3 showed extensive atelectasis.  In 18 of the 21 patients, paO2 increased after cannulation, FiO2 being kept unaltered.  As a consequence, the oxygen concentration in the inspired gas mixture could be lowered.  3 children had to be intubated endotracheally and ventilated artificially despite previous nasal cannulation.  The criteria for using the CPAP and the methodology are described, its advantages, disadvantages and dangers discussed."}
{"id": "PMID:1097895", "title": "Anatomy and histology of the human temporomandibular joint.", "content": "1. The anatomy and histology of the human temporomandibular joint indicates that the meniscus is firmly attached to the mandibular condyle and that during translatory movements the meniscus and condyle move concomitantly. The lateral pterygoid muscle, both inferior and superior heads insert into the meniscus and the pterygoid fovea of the condyle. 2. The structure of the gracilis and pars posterior indicate that these are the parts of the meniscus that are exposed to friction during jaw movement. It is suggested that during hinge movement the sagittal crest of the condyle moves from underneath the pars posterior to underneath the pars gracilis. 3. The restraining ligament for the articulation appears to be the lateral ligament. Its function is to keep the articular surfaces relatively close together during movement and to restrain lateral and posterior movements of the condyle. Its intimate association with the deep fibres of the masseter muscle appears to be important. 4. The fact that the meniscus is thicker medially than laterally would indicate that its major function is to smooth out the incongruities between the joint surfaces. 5. The sphenomandibular ligament as well as being a 'guy' ligament is intimately associated with the postero-superior medial capsular meniscal complex. The medial capsular complex would seem to contribute to the major part of the anterior ligament to the malleus. 6. Remodelling of the articular surfaces occur throughout life by progressive and regressive remodelling. However, if the remodelling processes increase, either the medio-lateral or antero-posterior dimensions of the joint an osteoarthritic condition prevails. 7. Excessive progressive remodelling appears to be due to abnormal positions of the condyle in the maximal intercuspal position. It can be caused by orthodontic procedures or by experimental displacement of the condyle. 8. The transitional zone appears to be important in the aetiology of osteoarthritis since when this zone comes under pressure proliferation of cartilage cells occur which may be transformed into bone, resulting in the hooked condyle.", "contents": "Anatomy and histology of the human temporomandibular joint. 1. The anatomy and histology of the human temporomandibular joint indicates that the meniscus is firmly attached to the mandibular condyle and that during translatory movements the meniscus and condyle move concomitantly. The lateral pterygoid muscle, both inferior and superior heads insert into the meniscus and the pterygoid fovea of the condyle. 2. The structure of the gracilis and pars posterior indicate that these are the parts of the meniscus that are exposed to friction during jaw movement. It is suggested that during hinge movement the sagittal crest of the condyle moves from underneath the pars posterior to underneath the pars gracilis. 3. The restraining ligament for the articulation appears to be the lateral ligament. Its function is to keep the articular surfaces relatively close together during movement and to restrain lateral and posterior movements of the condyle. Its intimate association with the deep fibres of the masseter muscle appears to be important. 4. The fact that the meniscus is thicker medially than laterally would indicate that its major function is to smooth out the incongruities between the joint surfaces. 5. The sphenomandibular ligament as well as being a 'guy' ligament is intimately associated with the postero-superior medial capsular meniscal complex. The medial capsular complex would seem to contribute to the major part of the anterior ligament to the malleus. 6. Remodelling of the articular surfaces occur throughout life by progressive and regressive remodelling. However, if the remodelling processes increase, either the medio-lateral or antero-posterior dimensions of the joint an osteoarthritic condition prevails. 7. Excessive progressive remodelling appears to be due to abnormal positions of the condyle in the maximal intercuspal position. It can be caused by orthodontic procedures or by experimental displacement of the condyle. 8. The transitional zone appears to be important in the aetiology of osteoarthritis since when this zone comes under pressure proliferation of cartilage cells occur which may be transformed into bone, resulting in the hooked condyle."}
{"id": "PMID:1097896", "title": "Mandibular movements.", "content": "The study of jaw movements or mandibular kinematics has important applications in many fields of clinical dentistry and provides a basis for modern concepts of dental occlusion. Movement patterns must be considered in conjunction with the mandibular articulation, the system of synovial joints, muscles and associated structures concerned with jaw suspension and jaw movements. Kinematics includes the study of articulating surfaces and the movements between them as well as the paths mapped out by a point on the moving bone. In this review, joint movements are considered from a theoretical approach based on the mechanics of articular surfaces, and by reference to recent experimental studies of condylar motion. Movements of the mandible are related to the envelope of motion traced out by the central incisor point. Attention is also given to border paths, reference positions and masticatory movements of the jaw.", "contents": "Mandibular movements. The study of jaw movements or mandibular kinematics has important applications in many fields of clinical dentistry and provides a basis for modern concepts of dental occlusion. Movement patterns must be considered in conjunction with the mandibular articulation, the system of synovial joints, muscles and associated structures concerned with jaw suspension and jaw movements. Kinematics includes the study of articulating surfaces and the movements between them as well as the paths mapped out by a point on the moving bone. In this review, joint movements are considered from a theoretical approach based on the mechanics of articular surfaces, and by reference to recent experimental studies of condylar motion. Movements of the mandible are related to the envelope of motion traced out by the central incisor point. Attention is also given to border paths, reference positions and masticatory movements of the jaw."}
{"id": "PMID:1097907", "title": "Study of genetic effects of high energy radiations with different ionizing capacities on extracellular phages.", "content": "The inactivating and mutagenic action of high-energy radiations with different ionizing capacities (gamma-rays, protons, alpha-particles and accelerated ions of 12C and 20Ne) was studied by using coliphages lambda11 and SD as subjects. In particular the role of irradiation conditions (broth suspension, pure buffer, dry samples) and of the host functions recA, exrA and polA was investigated. The dose-response curve of induced mutagenesis was studied by measuring the yield of vir mutants in lambda11 and plaque mutants in SD. The following results were obtained. (1) The inactivation kinetics of phages under the action of gamma-rays and protons was first order to a survival of 10(-7). Heavy ions also showed exponential inactivation kinetics to a survival of 10(-4). At higher doses of 20Ne ion bombardment some deviation from one-hit kinetics was observed. For dry samples of phages the dimensions of targets for all types of radiation were approximately proportional to the molecular weights of phage DNA's. For densely ionizing radiation (heavy ions) the inactivating action was 3-5 times weaker than for gamma-rays and protons. (2) Mutagenesis was observed for all types of radiation, but heavy ions were 1-5-2 times less efficient than gamma-rays. For both phages studied the dose-response curve of mutagenesis was non-linear. The dependence on the dose was near to parabolic for lambda11. For SD a plateau or maximum of mutagenesis was observed for the relative number of mutants at a survival of about 10(-4). (3) Host-cell functions recA and exrA were practically indifferent for survival of gamma-irradiated phage lambda11, but indispensable for mutagenesis. Mutation recAI3 abolished induced vir mutations totally and exrA- reduced them significantly. The absence of the function polA had a considerable influence on phage survival, but no effect on vir mutation yield (if compared at the same survival level). (4) In conditions of indirect action of gamma-rays no vir mutations were induced. This is regarded as evidence that the single-strand breaks formed under indirect action conditions cannot serve as pre-mutational damage in DNA.", "contents": "Study of genetic effects of high energy radiations with different ionizing capacities on extracellular phages. The inactivating and mutagenic action of high-energy radiations with different ionizing capacities (gamma-rays, protons, alpha-particles and accelerated ions of 12C and 20Ne) was studied by using coliphages lambda11 and SD as subjects. In particular the role of irradiation conditions (broth suspension, pure buffer, dry samples) and of the host functions recA, exrA and polA was investigated. The dose-response curve of induced mutagenesis was studied by measuring the yield of vir mutants in lambda11 and plaque mutants in SD. The following results were obtained. (1) The inactivation kinetics of phages under the action of gamma-rays and protons was first order to a survival of 10(-7). Heavy ions also showed exponential inactivation kinetics to a survival of 10(-4). At higher doses of 20Ne ion bombardment some deviation from one-hit kinetics was observed. For dry samples of phages the dimensions of targets for all types of radiation were approximately proportional to the molecular weights of phage DNA's. For densely ionizing radiation (heavy ions) the inactivating action was 3-5 times weaker than for gamma-rays and protons. (2) Mutagenesis was observed for all types of radiation, but heavy ions were 1-5-2 times less efficient than gamma-rays. For both phages studied the dose-response curve of mutagenesis was non-linear. The dependence on the dose was near to parabolic for lambda11. For SD a plateau or maximum of mutagenesis was observed for the relative number of mutants at a survival of about 10(-4). (3) Host-cell functions recA and exrA were practically indifferent for survival of gamma-irradiated phage lambda11, but indispensable for mutagenesis. Mutation recAI3 abolished induced vir mutations totally and exrA- reduced them significantly. The absence of the function polA had a considerable influence on phage survival, but no effect on vir mutation yield (if compared at the same survival level). (4) In conditions of indirect action of gamma-rays no vir mutations were induced. This is regarded as evidence that the single-strand breaks formed under indirect action conditions cannot serve as pre-mutational damage in DNA."}
{"id": "PMID:1097910", "title": "Phileomycin-induced lethality and DNA degradation in Escherichia coli K12.", "content": "The cell lethality and DNA fragmentation caused by phleomycin (PM) were studied in E. coli K12 strains with special reference to the effects of repair or recombination deficiences and metabolic inhibitors. (1) Unlike excision-defective derivatives of E. coli B, uvrA, uvrB, and uvrC mutants of strain K12 showed no peculiarities compared with wild type in regard to cell survival. Likewise, mutant alleles at uvrD and polA loci had no effect. In contrast, rec mutants were more sensitive to PM-killing than were rec+ strains. (2) PM-induced strand breakage in DNA was observed in all strains tested including the above-mentioned mutants. There was no significant distinction between the uvr mutants and the wild type strain, indicating that the uvr-endonuclease was not responsible for the strand breaks. Involvement of endonuclease I was also ruled out. (3) At least some of the PM-induced breaks were repairable. (4) PM-induced lethality and strand breakage were totally dependent on energy supply. Inhibition of protein synthesis resulted in a partial and parallel suppression of the two effects. Our results suggest that the lethality is due to DNA strand breakage and the repair of such damage is postulated to be controlled by rec genes.", "contents": "Phileomycin-induced lethality and DNA degradation in Escherichia coli K12. The cell lethality and DNA fragmentation caused by phleomycin (PM) were studied in E. coli K12 strains with special reference to the effects of repair or recombination deficiences and metabolic inhibitors. (1) Unlike excision-defective derivatives of E. coli B, uvrA, uvrB, and uvrC mutants of strain K12 showed no peculiarities compared with wild type in regard to cell survival. Likewise, mutant alleles at uvrD and polA loci had no effect. In contrast, rec mutants were more sensitive to PM-killing than were rec+ strains. (2) PM-induced strand breakage in DNA was observed in all strains tested including the above-mentioned mutants. There was no significant distinction between the uvr mutants and the wild type strain, indicating that the uvr-endonuclease was not responsible for the strand breaks. Involvement of endonuclease I was also ruled out. (3) At least some of the PM-induced breaks were repairable. (4) PM-induced lethality and strand breakage were totally dependent on energy supply. Inhibition of protein synthesis resulted in a partial and parallel suppression of the two effects. Our results suggest that the lethality is due to DNA strand breakage and the repair of such damage is postulated to be controlled by rec genes."}
{"id": "PMID:1097911", "title": "Induction of rho-minus mutants in Saccharomyces cerevisiae by guanidine hydrochloride. I. Genetic analysis.", "content": "Guanidine hydrochloride (GuHCl) induced in Saccharomyces cerevisiae cytoplasmic petite mutants (rho-) of the suppressive type. However, it was unable to induce the neutral type, even after prolonged incubation or increased drug concentration. No correlation was found between the degree of suppressiveness and the time of incubation of yeast cells with guanidine hydrochloride. The suppressiveness of rho- induced was not altered by further treatment with GuHCl, whereas it was reduced upon treatment with ethidium bromide (EtBr). Some mitochondrial genetic information was lacking in the rho- mutants induced by GuHCl, as demonstrated by the loss of the gene for erythromycin resistance and by reduced buoyant density of mitochondrial DNA of some rho-. There was no correlation between the degree of suppressiveness of the rho- induced by GuHCl and the buoyant density of the mutant mitochondrial DNA.", "contents": "Induction of rho-minus mutants in Saccharomyces cerevisiae by guanidine hydrochloride. I. Genetic analysis. Guanidine hydrochloride (GuHCl) induced in Saccharomyces cerevisiae cytoplasmic petite mutants (rho-) of the suppressive type. However, it was unable to induce the neutral type, even after prolonged incubation or increased drug concentration. No correlation was found between the degree of suppressiveness and the time of incubation of yeast cells with guanidine hydrochloride. The suppressiveness of rho- induced was not altered by further treatment with GuHCl, whereas it was reduced upon treatment with ethidium bromide (EtBr). Some mitochondrial genetic information was lacking in the rho- mutants induced by GuHCl, as demonstrated by the loss of the gene for erythromycin resistance and by reduced buoyant density of mitochondrial DNA of some rho-. There was no correlation between the degree of suppressiveness of the rho- induced by GuHCl and the buoyant density of the mutant mitochondrial DNA."}
{"id": "PMID:1097914", "title": "Role of toxigenic and invasive bacteria in acute diarrhea of childhood.", "content": "The cause of pediatric diarrhea in Brazil was investigated with use of assays for toxigenic and invasive bacteria. Potential etiologic agents were identified in 31 of 40 consecutive children with diarrhea: toxigenic Escherichia coli or klebsiella in 20 patients (50 per cent); invasive Esch, coli or salmonella in four (10 per cent), and both toxigenic and invasive organisms in seven (18 per cent). Fecal leukocytes were present in nine of the 11 patients (82 per cent) with invasive pathogens. All 27 patients with toxigenic isolates had organisms that produced heat-labile cholera-like toxin; three patients had Esch. coli that also produced a heat-stable toxin. Among 20 controls without diarrhea there were no organisms that produced heat-labile toxin or tissue invasion, but there was one Esch. coli that produced only heat-stable toxin and one salmonella. In this pediatric population Esch. coli appears to cause most acute diarrheas due to toxigenic and invasive pathogenic mechanisms.", "contents": "Role of toxigenic and invasive bacteria in acute diarrhea of childhood. The cause of pediatric diarrhea in Brazil was investigated with use of assays for toxigenic and invasive bacteria. Potential etiologic agents were identified in 31 of 40 consecutive children with diarrhea: toxigenic Escherichia coli or klebsiella in 20 patients (50 per cent); invasive Esch, coli or salmonella in four (10 per cent), and both toxigenic and invasive organisms in seven (18 per cent). Fecal leukocytes were present in nine of the 11 patients (82 per cent) with invasive pathogens. All 27 patients with toxigenic isolates had organisms that produced heat-labile cholera-like toxin; three patients had Esch. coli that also produced a heat-stable toxin. Among 20 controls without diarrhea there were no organisms that produced heat-labile toxin or tissue invasion, but there was one Esch. coli that produced only heat-stable toxin and one salmonella. In this pediatric population Esch. coli appears to cause most acute diarrheas due to toxigenic and invasive pathogenic mechanisms."}
{"id": "PMID:1097916", "title": "The future of chiropractic: a psychosocial view.", "content": "As the total health-care delivery system in the United States has come under increasing public scrutiny, the role of chiropractic has become of interest. An overview of chiropractic, with an analysis of its social functions and a projection of its future indicates that chiropractic is more congruent with the values of its lower-middle-class clientele than is orthodox medicine. Chiropractors function adquately to legitimize their patients' entrance into the \"sick role\" and to provide an alternative channel to therapy. The marginal role of the chiropractor in the health-care system, as compared to that of the physician, is stable and functional for the chiropractors thenselves, as well as for their patients. Chiropractic likewise serves the physician, both as a boundary-defining mechanism and as a means of disengagement from troublesome patients. For these reasons, we believe that the role of chiropractic within the health-care system will remain stable in the future.", "contents": "The future of chiropractic: a psychosocial view. As the total health-care delivery system in the United States has come under increasing public scrutiny, the role of chiropractic has become of interest. An overview of chiropractic, with an analysis of its social functions and a projection of its future indicates that chiropractic is more congruent with the values of its lower-middle-class clientele than is orthodox medicine. Chiropractors function adquately to legitimize their patients' entrance into the \"sick role\" and to provide an alternative channel to therapy. The marginal role of the chiropractor in the health-care system, as compared to that of the physician, is stable and functional for the chiropractors thenselves, as well as for their patients. Chiropractic likewise serves the physician, both as a boundary-defining mechanism and as a means of disengagement from troublesome patients. For these reasons, we believe that the role of chiropractic within the health-care system will remain stable in the future."}
{"id": "PMID:1097921", "title": "Efficacy of acupuncture on osteoarthritic pain. A controlled, double-blind study.", "content": "Forty patients, randomly assigned to an experimental and a control group, participated in a double-blind study to assess the effectiveness of acupuncture in reducing chronic pain associated with osteoarthritis.  The experimental group received treatment at standard acupuncture points, and the control group at placebo points.  Analysis before and after treatment showed a significant (P less than 0.05) improvement in tenderness and subjective report of pain in both groups as evaluated by two independent observers and in activity by one observer.  Comparison of responses to treatment between the two groups showed no significant (P greater than 0.05) difference.  Thus, both experimental and control groups showed a reduction in pain after the treatments. These results may reflect the natural course of illness, and various attitudinal and social factors.", "contents": "Efficacy of acupuncture on osteoarthritic pain. A controlled, double-blind study. Forty patients, randomly assigned to an experimental and a control group, participated in a double-blind study to assess the effectiveness of acupuncture in reducing chronic pain associated with osteoarthritis.  The experimental group received treatment at standard acupuncture points, and the control group at placebo points.  Analysis before and after treatment showed a significant (P less than 0.05) improvement in tenderness and subjective report of pain in both groups as evaluated by two independent observers and in activity by one observer.  Comparison of responses to treatment between the two groups showed no significant (P greater than 0.05) difference.  Thus, both experimental and control groups showed a reduction in pain after the treatments. These results may reflect the natural course of illness, and various attitudinal and social factors."}
{"id": "PMID:1097925", "title": "Propranolol-withdrawal rebound phenomenon. Exacerbation of coronary events after abrupt cessation of antianginal therapy.", "content": "Effects on anginal symptoms of sudden withdrawal of large doses of propranolol or placebo were evaluated in 20 patients in a double-blind crossover efficacy trial requiring sudden cessation of the agent. With propranolol, 160 to 320 mg per day for six and 12 weeks, no patients had increased angina or nitroglycerin use, and there were no hospitalizations or deaths. However, within two weeks of discontinuance of propranolol, untoward ischemic events developed in 10 patients. Six had serious withdrawal complications: intermediate coronary syndrome in three, and ventricular tachycardia, fatal myocardial infarction, and sudden death in one each. In four patients discontinuance of placebo increased anginal symptoms; in the remaining 10, ischemic symptoms were not provoked. The rebound phenomenon was related to degree of pre-propranolol angina and relief of pain by the agent. Thus, chronically administered propranolol should be gradually reduced, and activity restricted during its withdrawal.", "contents": "Propranolol-withdrawal rebound phenomenon. Exacerbation of coronary events after abrupt cessation of antianginal therapy. Effects on anginal symptoms of sudden withdrawal of large doses of propranolol or placebo were evaluated in 20 patients in a double-blind crossover efficacy trial requiring sudden cessation of the agent. With propranolol, 160 to 320 mg per day for six and 12 weeks, no patients had increased angina or nitroglycerin use, and there were no hospitalizations or deaths. However, within two weeks of discontinuance of propranolol, untoward ischemic events developed in 10 patients. Six had serious withdrawal complications: intermediate coronary syndrome in three, and ventricular tachycardia, fatal myocardial infarction, and sudden death in one each. In four patients discontinuance of placebo increased anginal symptoms; in the remaining 10, ischemic symptoms were not provoked. The rebound phenomenon was related to degree of pre-propranolol angina and relief of pain by the agent. Thus, chronically administered propranolol should be gradually reduced, and activity restricted during its withdrawal."}
{"id": "PMID:1097926", "title": "Demonstration of an intrathymic defect in a case of severe combined immunodeficiency disease.", "content": "An in vitro system has been developed to elucidate the nature of the cellular defect in primary immunodeficiency diseases. Incubation, on human thymic epithelial monolayer cultures, of peripheral blood lymphocytes and bone-marrow cells from a child with documented severe combined immunodeficiency disease resulted in the appearance of a population of cells that formed rosettes with sheep erythrocytes. The same cell preparation permitted the synthesis of antigen-specific, complement-dependent antibodies after in vitro education, as demonstrated in a plaque assay system. In addition, thymic tissue from the same child gave morphologic and functional evidence of maturation when cultured in vitro. The experimental results suggest that in this case, lymphoid precursor cells were present in the bone marrow but failed to differentiate to functional maturity due to a defect in maturation of thymic tissue.", "contents": "Demonstration of an intrathymic defect in a case of severe combined immunodeficiency disease. An in vitro system has been developed to elucidate the nature of the cellular defect in primary immunodeficiency diseases. Incubation, on human thymic epithelial monolayer cultures, of peripheral blood lymphocytes and bone-marrow cells from a child with documented severe combined immunodeficiency disease resulted in the appearance of a population of cells that formed rosettes with sheep erythrocytes. The same cell preparation permitted the synthesis of antigen-specific, complement-dependent antibodies after in vitro education, as demonstrated in a plaque assay system. In addition, thymic tissue from the same child gave morphologic and functional evidence of maturation when cultured in vitro. The experimental results suggest that in this case, lymphoid precursor cells were present in the bone marrow but failed to differentiate to functional maturity due to a defect in maturation of thymic tissue."}
{"id": "PMID:1097931", "title": "Fatal aspergillosis in imported parrots.", "content": "Spontaneous fatal aspergillosis occurred in several species of parrots imported from Latin America, Australia, Malaya and Ghana for studies on the control of psittacosis. Over a period of 4 years, 655 parrots were imported for use in these studies. All birds that died during these investigations were necropsied, and the internal organs of 45 were found to have macroscopic lesions suggestive of aspergillosis. Of these 45 suspected cases, 32 were confirmed as aspergillosis by both histopathology and culture, and three others by histopathology alone. There was no evidence that the remaining 10 had this disease. Of the 32 culturally confirmed cases, 13 were found to be caused by Aspergillus fumigatus, 16 by A. oryzae, and three by both fungi. In this series, three sets of circumstances appear to have been associated with the development of fatal aspergillosis. Their capture and transport to the United States, the administration of chlortetracycline used in the control of psittacosis, and the administration of cortisone acetate in an attempt to activate existent latent psittacosis infections. The possible causal relationship of these factors are discussed.", "contents": "Fatal aspergillosis in imported parrots. Spontaneous fatal aspergillosis occurred in several species of parrots imported from Latin America, Australia, Malaya and Ghana for studies on the control of psittacosis. Over a period of 4 years, 655 parrots were imported for use in these studies. All birds that died during these investigations were necropsied, and the internal organs of 45 were found to have macroscopic lesions suggestive of aspergillosis. Of these 45 suspected cases, 32 were confirmed as aspergillosis by both histopathology and culture, and three others by histopathology alone. There was no evidence that the remaining 10 had this disease. Of the 32 culturally confirmed cases, 13 were found to be caused by Aspergillus fumigatus, 16 by A. oryzae, and three by both fungi. In this series, three sets of circumstances appear to have been associated with the development of fatal aspergillosis. Their capture and transport to the United States, the administration of chlortetracycline used in the control of psittacosis, and the administration of cortisone acetate in an attempt to activate existent latent psittacosis infections. The possible causal relationship of these factors are discussed."}
{"id": "PMID:1097932", "title": "The ultrastructure of Prototheca wickerhamii.", "content": "Two clinical isolates from Prototheca wickerhamii were freeze-dried, fixed and studied by electron microscopy and were also examined growing in culture medium by phase contrast light microscopy. Resting spores placed on fresh medium developed cytoplasmic extensions which sequestrated before proliferation occurred. In the presence of adequate nutrients vegetative spores grew and subdivided to form up to 12 endospores within large sporangia which ruptured to release free spores every 5--6 hours. These proliferating or vegetative spores contained much more endoplasmic reticulum and mitochondria than the resting spores which contained more lipid, and often starch granules as well, but relatively few membranous organelles.", "contents": "The ultrastructure of Prototheca wickerhamii. Two clinical isolates from Prototheca wickerhamii were freeze-dried, fixed and studied by electron microscopy and were also examined growing in culture medium by phase contrast light microscopy. Resting spores placed on fresh medium developed cytoplasmic extensions which sequestrated before proliferation occurred. In the presence of adequate nutrients vegetative spores grew and subdivided to form up to 12 endospores within large sporangia which ruptured to release free spores every 5--6 hours. These proliferating or vegetative spores contained much more endoplasmic reticulum and mitochondria than the resting spores which contained more lipid, and often starch granules as well, but relatively few membranous organelles."}
{"id": "PMID:1097949", "title": "Trichlortriethylamine (TS-160 Spofa) in combination chemotherapy of malignant lymphomas.", "content": "Thirteen patients with disseminated malignant lymphomas were treated with combination chemotherapy which included trichlortriethyl amine (TS-160), vincristine (Oncovin), procarbazine hydrochloride (Natulan) and prednisone. All patients but two received some form of chemotherapy and/or radiotherapy prior to being started on four-drug regimen. Good response to the combination chemotherapy was recorded in 84% of patients, while complete remission was achieved in 23% of cases. Drug toxicity has not proved to be a major problem in the combination used. Nausea and vomiting were the side effects the most frequently observed.", "contents": "Trichlortriethylamine (TS-160 Spofa) in combination chemotherapy of malignant lymphomas. Thirteen patients with disseminated malignant lymphomas were treated with combination chemotherapy which included trichlortriethyl amine (TS-160), vincristine (Oncovin), procarbazine hydrochloride (Natulan) and prednisone. All patients but two received some form of chemotherapy and/or radiotherapy prior to being started on four-drug regimen. Good response to the combination chemotherapy was recorded in 84% of patients, while complete remission was achieved in 23% of cases. Drug toxicity has not proved to be a major problem in the combination used. Nausea and vomiting were the side effects the most frequently observed."}
{"id": "PMID:1097950", "title": "Beneficial effect of hemodialysis on renal allograft survival.", "content": "The effect of the length of hemodialysis on the outcome of renal transplant was evaluated in 43 recipients of renal allograft. 22 out of 23 patients (95.7%) dialyzed for longer than one year had an excellent renal function at one year, while only 10 out of 20 patients (50%) dialyzed for less than one year had good renal function at one year. Only one kidney (4.3%) was lost due to rejection in the first group, while 10 kidneys (50%) failed to function because of severe rejection in the second group. The length of hemodialysis appears to have a beneficial effect on graft survival, but the mechanism by which it exerts this effect is not clear.", "contents": "Beneficial effect of hemodialysis on renal allograft survival. The effect of the length of hemodialysis on the outcome of renal transplant was evaluated in 43 recipients of renal allograft. 22 out of 23 patients (95.7%) dialyzed for longer than one year had an excellent renal function at one year, while only 10 out of 20 patients (50%) dialyzed for less than one year had good renal function at one year. Only one kidney (4.3%) was lost due to rejection in the first group, while 10 kidneys (50%) failed to function because of severe rejection in the second group. The length of hemodialysis appears to have a beneficial effect on graft survival, but the mechanism by which it exerts this effect is not clear."}
{"id": "PMID:1097951", "title": "Salmonella typhimurium infections in renal transplant patients. Report of five cases.", "content": "Five patients with Salmonella typhimurium infection after a renal cadaveric graft are described. Salmonella were isolated from the urine of four patients, from the stool of one patient, from the blood of two patients, from the hip joint of one patient and from the cerebrospinal fluid of one patient. Infections were difficult to eradicate and necessitated prolonged antibiotic treatment. Renal function only deteriorated after the infection in some patients; salmonellosis could have triggered the rejection of the graft. Impaired cell-mediated immunity due to immunosuppressive drugs may be considered to be a predisposing factor for this kind of infection. Higher humoral antibody titers against Salmonella were found in the patients most clinically ill.", "contents": "Salmonella typhimurium infections in renal transplant patients. Report of five cases. Five patients with Salmonella typhimurium infection after a renal cadaveric graft are described. Salmonella were isolated from the urine of four patients, from the stool of one patient, from the blood of two patients, from the hip joint of one patient and from the cerebrospinal fluid of one patient. Infections were difficult to eradicate and necessitated prolonged antibiotic treatment. Renal function only deteriorated after the infection in some patients; salmonellosis could have triggered the rejection of the graft. Impaired cell-mediated immunity due to immunosuppressive drugs may be considered to be a predisposing factor for this kind of infection. Higher humoral antibody titers against Salmonella were found in the patients most clinically ill."}
{"id": "PMID:1097954", "title": "[Controlled respiration in patients with severe neurological diseases. Syndrome of functional changes].", "content": "The investigations were carried out on a group of neurological patients treated by means of assisted or controlled respiration from respirators. Volumetric and gasometric measurements were performed and radiological examinations of the chest were done periodically. The obtained results suggest that neurological patients constitute a specific group with a more rapid fall in the values of VC and PaO2, increased alveolar-capillary gradient (PA-a) and decreased actual compliance delta t/ delta p.", "contents": "[Controlled respiration in patients with severe neurological diseases. Syndrome of functional changes]. The investigations were carried out on a group of neurological patients treated by means of assisted or controlled respiration from respirators. Volumetric and gasometric measurements were performed and radiological examinations of the chest were done periodically. The obtained results suggest that neurological patients constitute a specific group with a more rapid fall in the values of VC and PaO2, increased alveolar-capillary gradient (PA-a) and decreased actual compliance delta t/ delta p."}
{"id": "PMID:1097971", "title": "Bacturcult in the diagnosis of urinary tract infection.", "content": "Colony counts on 122 clean-catch mid-stream urine specimens were determined with Bacturcult and compared with those obtained using the quantitative pour plate method. The suggested criteria for using Bacturcult resulted in an unacceptably high number of false positive cultures. It has been shown that over 100 colonies in the proscribed area on the cellophane sleeve correlated well with pour plate colony counts of over 100 000/ml. Bacturcult was found to be a simple and convenient method for performing a quantitative colony count on a urine specimen. It could be recommended for use in domiciliary practice, out-patient clinics and in population screening programes.", "contents": "Bacturcult in the diagnosis of urinary tract infection. Colony counts on 122 clean-catch mid-stream urine specimens were determined with Bacturcult and compared with those obtained using the quantitative pour plate method. The suggested criteria for using Bacturcult resulted in an unacceptably high number of false positive cultures. It has been shown that over 100 colonies in the proscribed area on the cellophane sleeve correlated well with pour plate colony counts of over 100 000/ml. Bacturcult was found to be a simple and convenient method for performing a quantitative colony count on a urine specimen. It could be recommended for use in domiciliary practice, out-patient clinics and in population screening programes."}
{"id": "PMID:1097974", "title": "Hormonal profiles in anovulatory patients treated with gonadotropins and synthetic luteinizing hormone releasing hormone.", "content": "Ten patients with hypothalamic anovulation weretreated with a \"retard\" preparation of synthetic luteinizing hormone releasing hormone (LHRH) after an HMG stimulation in order to induce ovulation and pregnancy. Four of the patient ovulated after intramuscular administration of the LHRH preparation. This study suggests that is is possible to induce ovulation with LHRH in patients  pretreated with HMG, and that LHRH has advantages over HCG since it does not induce hyperstimulation even in the presence of exagerated follicular growth. Nevertheless, the optimal conditions for the use and monitoring of LHRH treatment have yet to be clarified.", "contents": "Hormonal profiles in anovulatory patients treated with gonadotropins and synthetic luteinizing hormone releasing hormone. Ten patients with hypothalamic anovulation weretreated with a \"retard\" preparation of synthetic luteinizing hormone releasing hormone (LHRH) after an HMG stimulation in order to induce ovulation and pregnancy. Four of the patient ovulated after intramuscular administration of the LHRH preparation. This study suggests that is is possible to induce ovulation with LHRH in patients  pretreated with HMG, and that LHRH has advantages over HCG since it does not induce hyperstimulation even in the presence of exagerated follicular growth. Nevertheless, the optimal conditions for the use and monitoring of LHRH treatment have yet to be clarified."}
{"id": "PMID:1097975", "title": "Chorioangiomas.", "content": "Six cases of chorioangioma of the placenta and a review of relevant leterature are presented. The incidence of chorioangiomas is higher than generally realized because many small chorioangiomas are missed in routine examination of placentas. A case of chorioangioma with foci resembling hemangiopericytoma is presented. Cases of large chorioangiomas seem to occur when the placenta is large. Hydramnios is commonly found with large chiorangiomas, and both of these conditions may be conducive to premature births. There is an increased incidence of toxemia in pregnant patients with chorioangiomas, and the condition is unrelated to the size of the tumor. Diligent examination of infants associated with chorioangioma of the placenta is suggested, as these infants may have unsuspected congenital anomalies.", "contents": "Chorioangiomas. Six cases of chorioangioma of the placenta and a review of relevant leterature are presented. The incidence of chorioangiomas is higher than generally realized because many small chorioangiomas are missed in routine examination of placentas. A case of chorioangioma with foci resembling hemangiopericytoma is presented. Cases of large chorioangiomas seem to occur when the placenta is large. Hydramnios is commonly found with large chiorangiomas, and both of these conditions may be conducive to premature births. There is an increased incidence of toxemia in pregnant patients with chorioangiomas, and the condition is unrelated to the size of the tumor. Diligent examination of infants associated with chorioangioma of the placenta is suggested, as these infants may have unsuspected congenital anomalies."}
{"id": "PMID:1097976", "title": "Transient renal dysfunction associated with acute pyelonephritis of pregnancy.", "content": "Endogenous creatinine clearance was measured in 220 pregnant women with acute pyelonephritis and the results compared to data obtained from 167 pregnant control patients without acute pyelonephritis. These data showed that an appreciable number of patients with pyelonephritis occurring during pregnancy demonstrated a transient but definite diminution in renal function during the acute attack. Causes of this temporary renal dysfunction are discussed.", "contents": "Transient renal dysfunction associated with acute pyelonephritis of pregnancy. Endogenous creatinine clearance was measured in 220 pregnant women with acute pyelonephritis and the results compared to data obtained from 167 pregnant control patients without acute pyelonephritis. These data showed that an appreciable number of patients with pyelonephritis occurring during pregnancy demonstrated a transient but definite diminution in renal function during the acute attack. Causes of this temporary renal dysfunction are discussed."}
{"id": "PMID:1097977", "title": "Priming of the uterine cervix with oral prostaglandin E2 in the term multigravida.", "content": "A double-blind study evaluating two dose schedules of oral prostaglandin E2 (PGE2) was conducted in 56 normal \"unripe\" multiparas at term. The effect of PGE2 was evaluated by comparing the change in Bishop score of the cervix and patient's response to oxytocin induction of labor after the prostaglandin priming. In the two study groups (one group on a fixed PGE2 dosage and the other on a variable drug regimen) an average change of more than three points in the Bishop score was observed. In control group no significant change in cervical ripeness was observed. Nine of the 32 patients (28%) receiving prostaglandin delivered during the priming phase of the study, whereas only 1 control patient (4.2%) delivered during priming. Six of 24 control patients (25%) failed to deliver, wheras only 3 of 32 study patients (9%) failed. In the successfully induced patients, the response to oxytocin, as expressed by the time to active phase, total time of labor, and mean dose of oxytocin required, did not vary significantly when control and study groups were compared. It is concluded that PGE2 at either dosage schedule is effective in changing the Bishop score of the cervix and that it is not feasible to titrate the PGE2 dose in order to effectively ripen the cervix without a very high incidence of induction of labor. In those cases where priming was completed, without accidental delivery, the induction phase was successful more than 90% of the time. No adverse effect to mother or baby was observed during this study.", "contents": "Priming of the uterine cervix with oral prostaglandin E2 in the term multigravida. A double-blind study evaluating two dose schedules of oral prostaglandin E2 (PGE2) was conducted in 56 normal \"unripe\" multiparas at term. The effect of PGE2 was evaluated by comparing the change in Bishop score of the cervix and patient's response to oxytocin induction of labor after the prostaglandin priming. In the two study groups (one group on a fixed PGE2 dosage and the other on a variable drug regimen) an average change of more than three points in the Bishop score was observed. In control group no significant change in cervical ripeness was observed. Nine of the 32 patients (28%) receiving prostaglandin delivered during the priming phase of the study, whereas only 1 control patient (4.2%) delivered during priming. Six of 24 control patients (25%) failed to deliver, wheras only 3 of 32 study patients (9%) failed. In the successfully induced patients, the response to oxytocin, as expressed by the time to active phase, total time of labor, and mean dose of oxytocin required, did not vary significantly when control and study groups were compared. It is concluded that PGE2 at either dosage schedule is effective in changing the Bishop score of the cervix and that it is not feasible to titrate the PGE2 dose in order to effectively ripen the cervix without a very high incidence of induction of labor. In those cases where priming was completed, without accidental delivery, the induction phase was successful more than 90% of the time. No adverse effect to mother or baby was observed during this study."}
{"id": "PMID:1097979", "title": "Repair of cicatricial ectropion by horizontal shortening and pedicle flap.", "content": "Senile ectropion can usually be repaired by a horizontal shortening procedure. However, in those cases where there is a cicatricial component, additional skin is needed to insure a satisfactory result. This report describes a method of correcting this type of ectropion by utilizing a combination of horizontal shortening and single pedicle flap from the upper eyelid.", "contents": "Repair of cicatricial ectropion by horizontal shortening and pedicle flap. Senile ectropion can usually be repaired by a horizontal shortening procedure. However, in those cases where there is a cicatricial component, additional skin is needed to insure a satisfactory result. This report describes a method of correcting this type of ectropion by utilizing a combination of horizontal shortening and single pedicle flap from the upper eyelid."}
{"id": "PMID:1097978", "title": "Complications after removal of mandibular third molars with special reference to local anaesthetics with different vasoactive properties.", "content": "Nhe symptoms after surgical removal of 379 mandibular wisdom teeth when Xylocain-Exadrin 2% and Citanest-Octopressin 3% were used, were evaluated according to a three grade scale. The severity of the symptoms, which were mild and normal in 53% and severe in 47%, did not demonstrably vary with the type of anaesthetic agent used. The symptoms were, on the average, more severe among the women than among the men. The symptoms were correlated with the duration of the operation, which was most obvious in the men. For the men the frequency of symptoms also increased with age. In the men there was also a tendency to a correlation between dry socket and long duration of operation. It thus appeared as if only the men reacted in the way expected from a biologic point of view. The investigation also showed that Xylocain-Exadrin had a better anaesthetic and ischemic effect.", "contents": "Complications after removal of mandibular third molars with special reference to local anaesthetics with different vasoactive properties. Nhe symptoms after surgical removal of 379 mandibular wisdom teeth when Xylocain-Exadrin 2% and Citanest-Octopressin 3% were used, were evaluated according to a three grade scale. The severity of the symptoms, which were mild and normal in 53% and severe in 47%, did not demonstrably vary with the type of anaesthetic agent used. The symptoms were, on the average, more severe among the women than among the men. The symptoms were correlated with the duration of the operation, which was most obvious in the men. For the men the frequency of symptoms also increased with age. In the men there was also a tendency to a correlation between dry socket and long duration of operation. It thus appeared as if only the men reacted in the way expected from a biologic point of view. The investigation also showed that Xylocain-Exadrin had a better anaesthetic and ischemic effect."}
{"id": "PMID:1097980", "title": "Genesis and genetics of retinoblastoma.", "content": "1. The increase in frequency of retinoblastoma reported during the last decades [FRANCOIS, 1968] is probably not due to an increase of the mutation rate but to a lowered mortality rate and also to improvement in methods of investigation and diagnosis. (2) In 95% of all affected families retinoblastoma occurs just once, the familial occurrence being +/- 5%. (3) The hereditary retinoblastomas (whether sporadic or familial) represent about 40% of all cases. (4) A new dominant mutation is judged to be responsible for 100% of the bilateral sporadic cases and for 10-20% of the unilateral sporadic cases. (5) Genetic susceptibility to cancer can be chromosomal, mendelian dominant, mendelian recessive, or polygenic. Retinoblastoma belongs to the first as well as to the second category. The fact that the chromosomal aberration of the D deletion syndrome antedates the appearance of the tumour suggests that chromosomal change may be the primary cause of the tumour formation. (6) Although several problems remain an unsolved question, attention must be given to KNUDSON'S working hypothesis [KNUDSON et al., 1973], according to which all childhood tumours fit at least a two-mutational aetiology, involving a two-step process. It remains an open question whether both events occur at different genetic loci or at alleles of the same locus. (7) The main difficulty in genetic counseling is the lack of means for identifying which sporadic retinoblastoma cases are due to new germinal mutations.", "contents": "Genesis and genetics of retinoblastoma. 1. The increase in frequency of retinoblastoma reported during the last decades [FRANCOIS, 1968] is probably not due to an increase of the mutation rate but to a lowered mortality rate and also to improvement in methods of investigation and diagnosis. (2) In 95% of all affected families retinoblastoma occurs just once, the familial occurrence being +/- 5%. (3) The hereditary retinoblastomas (whether sporadic or familial) represent about 40% of all cases. (4) A new dominant mutation is judged to be responsible for 100% of the bilateral sporadic cases and for 10-20% of the unilateral sporadic cases. (5) Genetic susceptibility to cancer can be chromosomal, mendelian dominant, mendelian recessive, or polygenic. Retinoblastoma belongs to the first as well as to the second category. The fact that the chromosomal aberration of the D deletion syndrome antedates the appearance of the tumour suggests that chromosomal change may be the primary cause of the tumour formation. (6) Although several problems remain an unsolved question, attention must be given to KNUDSON'S working hypothesis [KNUDSON et al., 1973], according to which all childhood tumours fit at least a two-mutational aetiology, involving a two-step process. It remains an open question whether both events occur at different genetic loci or at alleles of the same locus. (7) The main difficulty in genetic counseling is the lack of means for identifying which sporadic retinoblastoma cases are due to new germinal mutations."}
{"id": "PMID:1097981", "title": "[Radiation-induced sarcoma 12 years after high dosage x-ray treatment of a bilateral retinoblastoma].", "content": "After a survey of the literature, a radiation-induced sarcoma is described which had arisen 12 years after a successful high-dosage X-ray treatment of a bilateral retinoblastoma and led to the patient's death.", "contents": "[Radiation-induced sarcoma 12 years after high dosage x-ray treatment of a bilateral retinoblastoma]. After a survey of the literature, a radiation-induced sarcoma is described which had arisen 12 years after a successful high-dosage X-ray treatment of a bilateral retinoblastoma and led to the patient's death."}
{"id": "PMID:1097983", "title": "Reconstruction of the alveolar process by implantation of allogenic demineralized dentin.", "content": "Different materials have been used for reconstruction of the alveolar process. None of these, however, is perfect in every respect. Allogenic demineralized dentin has been shown to induce bone formation and to maintain the bone contour. In the present case this material was used to reconstruct a traumatic defect of the alveolar process in the region of the upper left incisors. The result was satisfactory, both esthetically and functionally. Additional cases and a longer period of observation will be necessary to assess the relevance of this finding.", "contents": "Reconstruction of the alveolar process by implantation of allogenic demineralized dentin. Different materials have been used for reconstruction of the alveolar process. None of these, however, is perfect in every respect. Allogenic demineralized dentin has been shown to induce bone formation and to maintain the bone contour. In the present case this material was used to reconstruct a traumatic defect of the alveolar process in the region of the upper left incisors. The result was satisfactory, both esthetically and functionally. Additional cases and a longer period of observation will be necessary to assess the relevance of this finding."}
{"id": "PMID:1097984", "title": "Cleidocranial dysostosis with mandibular cyst. Report of a case.", "content": "Six hundred cases of cleidocranial dysostosis have been reported since the disorder was first described in 1898. This is the first case reported with cystic involvement.", "contents": "Cleidocranial dysostosis with mandibular cyst. Report of a case. Six hundred cases of cleidocranial dysostosis have been reported since the disorder was first described in 1898. This is the first case reported with cystic involvement."}
{"id": "PMID:1097985", "title": "Nystatin and amphotericin B in the treatment of denture-related candidiasis.", "content": "A double-blind trial of nystatin, amphotericin B, and a placebo was carried out in fifty-two cases of denture-related candidiasis and/or angular cheilitis. The drugs effected a significant clinical cure, but recurrence of the signs was common after withdrawal of the drugs. Concurrent bacteriologic examination showed few cures and continued presence of Candida albicans during the trial. A specimen from a red palate was examined histologically.", "contents": "Nystatin and amphotericin B in the treatment of denture-related candidiasis. A double-blind trial of nystatin, amphotericin B, and a placebo was carried out in fifty-two cases of denture-related candidiasis and/or angular cheilitis. The drugs effected a significant clinical cure, but recurrence of the signs was common after withdrawal of the drugs. Concurrent bacteriologic examination showed few cures and continued presence of Candida albicans during the trial. A specimen from a red palate was examined histologically."}
{"id": "PMID:1097986", "title": "Klebsiella pneumoniae in endodontic therapy. Report of a case.", "content": "A 48-year-old man underwent endodontic treatment of the lower left central incisor. In the course of treatment two acute exacerbations occurred. Penicillin therapy was initiated at the time of the first exacerbation. It was ineffective, and a culture of the purulent exudate was taken at the time of the second acute response. The causative organism was Klebsiella pneumoniae, which was found to be sensitive to tetracyclines. After tetracycline therapy, endodontic therapy was completed. Healing has been uneventful.", "contents": "Klebsiella pneumoniae in endodontic therapy. Report of a case. A 48-year-old man underwent endodontic treatment of the lower left central incisor. In the course of treatment two acute exacerbations occurred. Penicillin therapy was initiated at the time of the first exacerbation. It was ineffective, and a culture of the purulent exudate was taken at the time of the second acute response. The causative organism was Klebsiella pneumoniae, which was found to be sensitive to tetracyclines. After tetracycline therapy, endodontic therapy was completed. Healing has been uneventful."}
{"id": "PMID:1097990", "title": "[Serum immunoglobulins in cardiovascular pathology].", "content": "The authors measured as a routine IgA, IgG and IgM serum immunoglobulins in a hundred subjects free from any cardiac or hypertensive disease, in 51 patients with coronary heart disease in in 50 hypertensive subjects. They found that in 2% of the normal subjects and in 28% of the coronary heart disease or hypertension, the level of one of the three serum immunoglobulins was outside the following limits: IgA 100 to 480 mg/100 ml, IgG 500 to 1900 mg/100 ml and IgM 45 to 380 mg/100 ml. The 2 normal subjects and 12% of the patients with coronary heart disease or hypertension presented an abnormally low level of one of the immunoglobulins, while an abnormally high level was observed in 16% of the subjects with coronary heart disease or hypertension. The abnormal serum levels may concern any class of immunoglobulin and persist in the case of some patients with coronary heart disease several years after the acute ischemic incident.", "contents": "[Serum immunoglobulins in cardiovascular pathology]. The authors measured as a routine IgA, IgG and IgM serum immunoglobulins in a hundred subjects free from any cardiac or hypertensive disease, in 51 patients with coronary heart disease in in 50 hypertensive subjects. They found that in 2% of the normal subjects and in 28% of the coronary heart disease or hypertension, the level of one of the three serum immunoglobulins was outside the following limits: IgA 100 to 480 mg/100 ml, IgG 500 to 1900 mg/100 ml and IgM 45 to 380 mg/100 ml. The 2 normal subjects and 12% of the patients with coronary heart disease or hypertension presented an abnormally low level of one of the immunoglobulins, while an abnormally high level was observed in 16% of the subjects with coronary heart disease or hypertension. The abnormal serum levels may concern any class of immunoglobulin and persist in the case of some patients with coronary heart disease several years after the acute ischemic incident."}
{"id": "PMID:1097991", "title": "Reptilase clot retraction test. (Its cofactors and relation to other platelet functions).", "content": "C-PRP clotted by reptilase does not retract due to the lack of activation of platelets. In order to support retraction, the platelets must undergo membrane changes resulting in contractile system activation. Only in the presence of aggregating agents capable of eliciting these membrane changes in vitro (e.g. ADP, adrenaline and collagen but not bovine fibrinogen or ristocetin), strong reptilase clot retraction (RCR) occurs. During RCR an aggregating activity, platelet factor 4, and retraction stimulating factor (RSF) were released, but acid phosphatase was not available. Not only inhibitors of platelet adhesion-aggregation reaction (PAAR) but also specific blockers of release are capable of inhibiting RCR. In coagulation disorders RCR was normal in most cases, but in thrombopathies different abnormalities of RCR were found. The RCR defect may be associated not only with a defect in the initial stages of PAAR, but in some instances also with a specific defect in release reaction. Besides inducer--\"activated\" platelets and their adhesion to polymerizing fibrin, the presence of divalent cations and free thrombocytar--SH groups is necessary for the retraction of reptilase clots.", "contents": "Reptilase clot retraction test. (Its cofactors and relation to other platelet functions). C-PRP clotted by reptilase does not retract due to the lack of activation of platelets. In order to support retraction, the platelets must undergo membrane changes resulting in contractile system activation. Only in the presence of aggregating agents capable of eliciting these membrane changes in vitro (e.g. ADP, adrenaline and collagen but not bovine fibrinogen or ristocetin), strong reptilase clot retraction (RCR) occurs. During RCR an aggregating activity, platelet factor 4, and retraction stimulating factor (RSF) were released, but acid phosphatase was not available. Not only inhibitors of platelet adhesion-aggregation reaction (PAAR) but also specific blockers of release are capable of inhibiting RCR. In coagulation disorders RCR was normal in most cases, but in thrombopathies different abnormalities of RCR were found. The RCR defect may be associated not only with a defect in the initial stages of PAAR, but in some instances also with a specific defect in release reaction. Besides inducer--\"activated\" platelets and their adhesion to polymerizing fibrin, the presence of divalent cations and free thrombocytar--SH groups is necessary for the retraction of reptilase clots."}
{"id": "PMID:1097992", "title": "[Should resistance to methicillin of RH mutant staphylococci be necessarily attributed to cephalosporins?].", "content": "The resistance to cephalosporins of 48 heterogeneous methicillin-resistant strains (\"RH\" mutants) of Staphylococcus pyogenes var. aureus is studied in vitro with various methods: sensitivity discs test on Mueller-Hinton medium with 5 per cent NaCl at 37 degrees C; M.I.C. in solid medium by spots medium with two inocula: a light inoculum (10(7) bacterial/ml) and a heavy inoculum (10(9) bacteria/ml). With light inoculum all strains have M.I.C. to cephaloridin less than 2 meg/ml and seem sensitive. With heavy inoculum 45 sc. equal or superior to 64 meg/ml and then are resistant. These resistances occur after 3 degrees C. The varied methods of detection of mutants to cephalosporins are discussed in this paper.", "contents": "[Should resistance to methicillin of RH mutant staphylococci be necessarily attributed to cephalosporins?]. The resistance to cephalosporins of 48 heterogeneous methicillin-resistant strains (\"RH\" mutants) of Staphylococcus pyogenes var. aureus is studied in vitro with various methods: sensitivity discs test on Mueller-Hinton medium with 5 per cent NaCl at 37 degrees C; M.I.C. in solid medium by spots medium with two inocula: a light inoculum (10(7) bacterial/ml) and a heavy inoculum (10(9) bacteria/ml). With light inoculum all strains have M.I.C. to cephaloridin less than 2 meg/ml and seem sensitive. With heavy inoculum 45 sc. equal or superior to 64 meg/ml and then are resistant. These resistances occur after 3 degrees C. The varied methods of detection of mutants to cephalosporins are discussed in this paper."}
{"id": "PMID:1097993", "title": "[Epidemiological study of antibiotic resistance of Escherichia coli strains isolated during one year at the regional hospital center of Angers. Trial of a statistical method].", "content": "A study of 1956 strains of Escherichia coli isolated at the Angers Regional Hospital Center during the year 1972, was used for the development of a statistical method of study of epidemiology of antibiotic resistance.", "contents": "[Epidemiological study of antibiotic resistance of Escherichia coli strains isolated during one year at the regional hospital center of Angers. Trial of a statistical method]. A study of 1956 strains of Escherichia coli isolated at the Angers Regional Hospital Center during the year 1972, was used for the development of a statistical method of study of epidemiology of antibiotic resistance."}
{"id": "PMID:1097994", "title": "[Electrophoretic study of normal erythrocyte membrane proteins after in vitro action of antimalarial agents].", "content": "The electrophretic pattern of the red cell membrane protein, after the in vitro action on them of antimalarial agents, primaquine, quinine and chloroquine, is described. This electrophoretic study has proved that all studied antimalarial agents after the membrane proteins, causing a total loss of certain bands. This method has proved to be a new way of appreciation of the action of different agents on the red cell membrane.", "contents": "[Electrophoretic study of normal erythrocyte membrane proteins after in vitro action of antimalarial agents]. The electrophretic pattern of the red cell membrane protein, after the in vitro action on them of antimalarial agents, primaquine, quinine and chloroquine, is described. This electrophoretic study has proved that all studied antimalarial agents after the membrane proteins, causing a total loss of certain bands. This method has proved to be a new way of appreciation of the action of different agents on the red cell membrane."}
{"id": "PMID:1097995", "title": "[Pulmonary blood volume. Definition, measurement, normal values].", "content": "We propose a simplification of the double-injection method whereby left atrial catheterization is made unnecessary by use of injection of indicator in the pulmonary wedge position instead of into the left atrium. Normal values of PBV range between 204 +/- 51 ml. m-2 and 326 +/- 51 ml. M-2. Such a large scatter is due in all likelihood partly to techniques themselves, partly to the small number of studied subjects, which precludes standardization of normal values according to age, sex and body build. Determination of PBV may help in assessing the overall distensibility of the pulmonary vascular bed, but is of no avail to assess separately the distensibility of arteries, microcirculation, and veins whose respective volumes cannot be measured in vivo for lack of undisputable methods. Morphometric and physiological observations suggest that the measurement of PBV is no avail to estimate changes in caliber of pulmonary arterioles caused by vasoactive stimuli.", "contents": "[Pulmonary blood volume. Definition, measurement, normal values]. We propose a simplification of the double-injection method whereby left atrial catheterization is made unnecessary by use of injection of indicator in the pulmonary wedge position instead of into the left atrium. Normal values of PBV range between 204 +/- 51 ml. m-2 and 326 +/- 51 ml. M-2. Such a large scatter is due in all likelihood partly to techniques themselves, partly to the small number of studied subjects, which precludes standardization of normal values according to age, sex and body build. Determination of PBV may help in assessing the overall distensibility of the pulmonary vascular bed, but is of no avail to assess separately the distensibility of arteries, microcirculation, and veins whose respective volumes cannot be measured in vivo for lack of undisputable methods. Morphometric and physiological observations suggest that the measurement of PBV is no avail to estimate changes in caliber of pulmonary arterioles caused by vasoactive stimuli."}
{"id": "PMID:1097996", "title": "[Use of the plastic \"Terphane\" for the maintenance and the study of cell cultures].", "content": "Terphane plastic used as substrate for tissue culture, facilitates both cytochemcial, biochemical and ulstratructural studies of cells in situ. Particularly pliable, thin and resistant, the sheet of Terphane plastic is held tense by a system of two plaques of Petri dish type. This apparatus is perfectly waterproof, sterilisable and may be used indefinitely. Growth, morphology, ultrastructure and enzyme activities of primary or subcultures of rat and human liver maintained on terphane plastic are comparable with those of cells cultivated on Falcon polystyrene. Furthermore, terphane offers several advantages over the Melinex plastic: its thinness, resistance and plasticity render easy all manipulations.", "contents": "[Use of the plastic \"Terphane\" for the maintenance and the study of cell cultures]. Terphane plastic used as substrate for tissue culture, facilitates both cytochemcial, biochemical and ulstratructural studies of cells in situ. Particularly pliable, thin and resistant, the sheet of Terphane plastic is held tense by a system of two plaques of Petri dish type. This apparatus is perfectly waterproof, sterilisable and may be used indefinitely. Growth, morphology, ultrastructure and enzyme activities of primary or subcultures of rat and human liver maintained on terphane plastic are comparable with those of cells cultivated on Falcon polystyrene. Furthermore, terphane offers several advantages over the Melinex plastic: its thinness, resistance and plasticity render easy all manipulations."}
{"id": "PMID:1097999", "title": "The restoration of the vasculature of skin autografts in the rabbit.", "content": "The restoration of the vasculature of full-thickness skin autografts was investigated on the rabbit ear, the grafted discs of skin being replaced with their original orientation, or rotated 90 or 180 degrees. Circulation in the graft was first detected 1 day after grafting, increasing on the 2nd and successive days. The larger arteries in the grafts survived and became permanently joined at the periphery of the grafts to adjacent severed arteries in the host by well-defined and often tortuous segments termed junction vessels. The pre-operative vascular pattern was re-established when grafts were replaced without rotation but comparable survival of arteries also occurred when the grafted discs of skin were rotated. In most grafts, the segment of artery became stabilized by 4-9 weeks; more than 75% of the length of the arterial segments survived in 83% of grafts, with minimal accompanying histological changes in their walls. There was some increase in number and aborization of small vessels in the established grafts, although the vascular pattern was essentially similar to that of unoperated skin. The factors possibly involved in the survival of the large arteries in the grafts include the relative rigidity of the arterial wall, the early establishment of circulation, and the favourable haemodynamic position of the grafts.", "contents": "The restoration of the vasculature of skin autografts in the rabbit. The restoration of the vasculature of full-thickness skin autografts was investigated on the rabbit ear, the grafted discs of skin being replaced with their original orientation, or rotated 90 or 180 degrees. Circulation in the graft was first detected 1 day after grafting, increasing on the 2nd and successive days. The larger arteries in the grafts survived and became permanently joined at the periphery of the grafts to adjacent severed arteries in the host by well-defined and often tortuous segments termed junction vessels. The pre-operative vascular pattern was re-established when grafts were replaced without rotation but comparable survival of arteries also occurred when the grafted discs of skin were rotated. In most grafts, the segment of artery became stabilized by 4-9 weeks; more than 75% of the length of the arterial segments survived in 83% of grafts, with minimal accompanying histological changes in their walls. There was some increase in number and aborization of small vessels in the established grafts, although the vascular pattern was essentially similar to that of unoperated skin. The factors possibly involved in the survival of the large arteries in the grafts include the relative rigidity of the arterial wall, the early establishment of circulation, and the favourable haemodynamic position of the grafts."}
{"id": "PMID:1098000", "title": "Evaluation of two kinds of smallpox vaccine: CVI-78 and calf lymph vaccine. I. Clinical and serologic response to primary vaccination.", "content": "A comparative study of two smallpox vaccines, standard calf lymph vaccine, and an attenuated vaccine, CVI-78, was performed in 95 children. Primary vaccination with CVI-78 resulted in a more attenuated response than primary vaccination with standard vaccine. Sixty-one percent of those vaccinated with CVI-78 and 96 percent of those vaccinated with standard vaccine developed a major dermal reaction; 16 percent of those vaccinated with CVI-78 and 89 percent of those vaccinated with standard vaccine developed post-vaccination neutralizing antibodies. Twenty-seven percent of the children vaccinated with CVI-78 demonstrated neither a dermal nor serologic postvaccination response, whereas only 2 percent of those vaccinated with standard vaccination demonstrated no postvaccination response.", "contents": "Evaluation of two kinds of smallpox vaccine: CVI-78 and calf lymph vaccine. I. Clinical and serologic response to primary vaccination. A comparative study of two smallpox vaccines, standard calf lymph vaccine, and an attenuated vaccine, CVI-78, was performed in 95 children. Primary vaccination with CVI-78 resulted in a more attenuated response than primary vaccination with standard vaccine. Sixty-one percent of those vaccinated with CVI-78 and 96 percent of those vaccinated with standard vaccine developed a major dermal reaction; 16 percent of those vaccinated with CVI-78 and 89 percent of those vaccinated with standard vaccine developed post-vaccination neutralizing antibodies. Twenty-seven percent of the children vaccinated with CVI-78 demonstrated neither a dermal nor serologic postvaccination response, whereas only 2 percent of those vaccinated with standard vaccination demonstrated no postvaccination response."}
{"id": "PMID:1098002", "title": "Chronic vulvovaginitis in children due to Shigella flexneri.", "content": "Although previous reports have implicated Shigella flexneri in resistant or chronic cases  of vulvovaginitis in children, no authors have described the clinical findings of this condition. The report presents four cases of persistent vulvovaginitis in prepubertal Indian girls from different reservation communities in Arizona. S. flexneri was isolated in pure culture from the vaginal discharge of each patient. All four cases were characterized by a prolonged vaginitis with a bloody, purulent discharge which responded poorly or not at all to various topical modes of therapy for nonspecific vaginitis. Three cases cleared completely when treated with orally given ampicillin for one week. The striking similarity of these cases suggests that chronic Shigella vulvovaginitis is a recognizable clinical entity and should be considered in the differential diagnosis of persistent vaginitis in children, especially in those from communities where Shigella is endemic.", "contents": "Chronic vulvovaginitis in children due to Shigella flexneri. Although previous reports have implicated Shigella flexneri in resistant or chronic cases  of vulvovaginitis in children, no authors have described the clinical findings of this condition. The report presents four cases of persistent vulvovaginitis in prepubertal Indian girls from different reservation communities in Arizona. S. flexneri was isolated in pure culture from the vaginal discharge of each patient. All four cases were characterized by a prolonged vaginitis with a bloody, purulent discharge which responded poorly or not at all to various topical modes of therapy for nonspecific vaginitis. Three cases cleared completely when treated with orally given ampicillin for one week. The striking similarity of these cases suggests that chronic Shigella vulvovaginitis is a recognizable clinical entity and should be considered in the differential diagnosis of persistent vaginitis in children, especially in those from communities where Shigella is endemic."}
{"id": "PMID:1098003", "title": "An approach to the measurement of body fluid compartment volumes in non-steady conditions in the rat.", "content": "A method has been developed by which body fluid volume changes can be assessed frequently after only one initial injection of the appropriate radioactive tracers. This method is based on the assumptions that, 1. following their intravenous injection, the temporal behaviour of tritiated water or radio-sodium can be adequately modelled by the kinetic behaviour of an open, interconnected two-compartment system; 2. known amounts of tracer are added to or irreversibly removed from the system only via the first compartment. In the measurement of body fluid volume changes in rats after isotonic blood volume expansion, the rapid urinary tracer excretion was treated as a series of negative tracer \"injections\" made instantaneously into the first tracer compartment at the mid point of each short urine collection period. The effect of these \"injections\" on the first compartment was regarded as diminishing with time in accordance with the steady state rate constants. The vaolues for non steady state changes in total body water volume and functional extracellular fluid volume obtained by such a mathematical treatment, agreed closely with directly measured changes where such direct comparisons could be made.", "contents": "An approach to the measurement of body fluid compartment volumes in non-steady conditions in the rat. A method has been developed by which body fluid volume changes can be assessed frequently after only one initial injection of the appropriate radioactive tracers. This method is based on the assumptions that, 1. following their intravenous injection, the temporal behaviour of tritiated water or radio-sodium can be adequately modelled by the kinetic behaviour of an open, interconnected two-compartment system; 2. known amounts of tracer are added to or irreversibly removed from the system only via the first compartment. In the measurement of body fluid volume changes in rats after isotonic blood volume expansion, the rapid urinary tracer excretion was treated as a series of negative tracer \"injections\" made instantaneously into the first tracer compartment at the mid point of each short urine collection period. The effect of these \"injections\" on the first compartment was regarded as diminishing with time in accordance with the steady state rate constants. The vaolues for non steady state changes in total body water volume and functional extracellular fluid volume obtained by such a mathematical treatment, agreed closely with directly measured changes where such direct comparisons could be made."}
{"id": "PMID:1098004", "title": "On the regulation of the renal response blood volume expansion by vascular parameters in the rat.", "content": "The dynamic patterns of body fluid volume distribution, of cardiovascular variables and of renal water, sodium and potassium excretion were studied in the anaesthetized rat following acute, iso-oncotic blood volume expansion. The increased renal excretion following expansion was soon reversed and its pattern to termination was not correlated with changes in pulse and mean arterial pressure, in plasma electrolyte concentration or in haematocrit. At the end of the renal response the change in extracellular fluid volume was not usually corrected, the blood volume was always well above its control value and the interstitial fluid was below its control value. In all cases the temporal pattern and the termination of the renal response corresponded closely with the temporal pattern and the return to the control value of the central venous mean and pulse pressure. The findings of thse experiments are not consistent with the view that any of the measured body fluid volumes directly and always determine renal excretion. It is proposed that in response to an acute blood volume expansion an animal may initially regulate neither its blood volume nor its extracellular fluid volume but rather a factor which is reflected in or related to the central venous pressure.", "contents": "On the regulation of the renal response blood volume expansion by vascular parameters in the rat. The dynamic patterns of body fluid volume distribution, of cardiovascular variables and of renal water, sodium and potassium excretion were studied in the anaesthetized rat following acute, iso-oncotic blood volume expansion. The increased renal excretion following expansion was soon reversed and its pattern to termination was not correlated with changes in pulse and mean arterial pressure, in plasma electrolyte concentration or in haematocrit. At the end of the renal response the change in extracellular fluid volume was not usually corrected, the blood volume was always well above its control value and the interstitial fluid was below its control value. In all cases the temporal pattern and the termination of the renal response corresponded closely with the temporal pattern and the return to the control value of the central venous mean and pulse pressure. The findings of thse experiments are not consistent with the view that any of the measured body fluid volumes directly and always determine renal excretion. It is proposed that in response to an acute blood volume expansion an animal may initially regulate neither its blood volume nor its extracellular fluid volume but rather a factor which is reflected in or related to the central venous pressure."}
{"id": "PMID:1098010", "title": "[The effect of antibiotic supplementation on antibiotic resistance, transferable antibiotic resistance, morbidity, and growth in calves (author's transl)].", "content": "112 calves, divided into four groups, were given milk substitutes containing tetracycline (high-dosage feed), zinc bacitracin or Lactosat (control) and concentratesw with or without zinc bacitracin (Table I). We have investigated the incidence of antibiotic resistance and transferable antibiotic resistance in E. coli strains isolated from the faces of these calves and checked the growth and state of health in the four groups. Growth was also studies in a further ten feeding series comprising a total of 258 calves. Half this number received zinc bacitracin in milk substitute, the other half were fed milk substitute containing Lactosat, instead of antibiotics (Fig. 8). Tetracycline led to a quick increase of antibiotic-resistant straing (Fig. 4--6). This increase is significant in comparison with the other groups. Significant increases of resistance were also noted to ampicillin and chloramphenicol (Fig. 7), althoug these drugs were not used. A significant increase of the transferable resistance was also observed in the group of calves given tetracycline (Fig. 7) and to 65% at about 31/2 months after the end to 38% within 12 days (Fig. 7) and to 65% at about 31/2 months after the end of tetracycline feeding (Fig. 7). A marked increase of en-bloc transfer was also noted after completion of tetracycline feeding (Fig. 2). Up to slaughter, at the age of 6 months, no better growth was obtained with zinc-bacitracin supplement than with milk substitute without antibiotics but containing Lactosat. During the milking period the results varied between the different feeding series. In some series the mean growth in the zinc-bacitracin group was better than, in some no different from, and in other s poorer than that in the Lactosat group (TableII, Fig. 8). The only demonstrable positive effect of tetracycline supplement was a tendency twwards better health during the period of administration of the drug (Table II...", "contents": "[The effect of antibiotic supplementation on antibiotic resistance, transferable antibiotic resistance, morbidity, and growth in calves (author's transl)]. 112 calves, divided into four groups, were given milk substitutes containing tetracycline (high-dosage feed), zinc bacitracin or Lactosat (control) and concentratesw with or without zinc bacitracin (Table I). We have investigated the incidence of antibiotic resistance and transferable antibiotic resistance in E. coli strains isolated from the faces of these calves and checked the growth and state of health in the four groups. Growth was also studies in a further ten feeding series comprising a total of 258 calves. Half this number received zinc bacitracin in milk substitute, the other half were fed milk substitute containing Lactosat, instead of antibiotics (Fig. 8). Tetracycline led to a quick increase of antibiotic-resistant straing (Fig. 4--6). This increase is significant in comparison with the other groups. Significant increases of resistance were also noted to ampicillin and chloramphenicol (Fig. 7), althoug these drugs were not used. A significant increase of the transferable resistance was also observed in the group of calves given tetracycline (Fig. 7) and to 65% at about 31/2 months after the end to 38% within 12 days (Fig. 7) and to 65% at about 31/2 months after the end of tetracycline feeding (Fig. 7). A marked increase of en-bloc transfer was also noted after completion of tetracycline feeding (Fig. 2). Up to slaughter, at the age of 6 months, no better growth was obtained with zinc-bacitracin supplement than with milk substitute without antibiotics but containing Lactosat. During the milking period the results varied between the different feeding series. In some series the mean growth in the zinc-bacitracin group was better than, in some no different from, and in other s poorer than that in the Lactosat group (TableII, Fig. 8). The only demonstrable positive effect of tetracycline supplement was a tendency twwards better health during the period of administration of the drug (Table II..."}
{"id": "PMID:1098011", "title": "Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey.", "content": "Two mycoplasma species of the porcine respiratory tract: Mycoplasma suipneumoniae and Mycoplasma flocculare, have been notoriously difficult to cultivate. In the present paper a description is given of a new medium with which primary isolation of these organisms is usually successful. The basal medium is made from commercial dehydrated products. Pig serum is added and phenol red used as pH indicator. Contrary to what is customary in the preparation of mycoplasma media, various inorganic salts (Hank's balanced salt solution) are included and penicillin-G replaced as a bacteriostatic by bacitracin and meticillin. The volume of water is adjusted so as to give isotonia. Various additives often recommended for mycoplasma cultivation have been examined for growth promoting effect; apparently, however, they were all without effect. Working procedures for primary isolation trials are briefly described.", "contents": "Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey. Two mycoplasma species of the porcine respiratory tract: Mycoplasma suipneumoniae and Mycoplasma flocculare, have been notoriously difficult to cultivate. In the present paper a description is given of a new medium with which primary isolation of these organisms is usually successful. The basal medium is made from commercial dehydrated products. Pig serum is added and phenol red used as pH indicator. Contrary to what is customary in the preparation of mycoplasma media, various inorganic salts (Hank's balanced salt solution) are included and penicillin-G replaced as a bacteriostatic by bacitracin and meticillin. The volume of water is adjusted so as to give isotonia. Various additives often recommended for mycoplasma cultivation have been examined for growth promoting effect; apparently, however, they were all without effect. Working procedures for primary isolation trials are briefly described."}
{"id": "PMID:1098012", "title": "[The development of drug resistance in Salmonella strains isolated from animals in Sweden 1955-65 and 1973-4(author's transl)].", "content": "134 salmonella strains, isolated from animals in 1955-65 were compared to 133 salmonella strains isolated in 1973-74, with regard to their drug resistance and the frequentcy of R-factors. No significant changes were observed during the reference period. Salmonella typhi-murium in comparison with other serotypes harboured a significantly larger number of resistant strains. Of the recently isolated resistant strains that were searched for R-factors, about half the number were carriers. All strains were sensitive to chloramphenicole and neomycin.", "contents": "[The development of drug resistance in Salmonella strains isolated from animals in Sweden 1955-65 and 1973-4(author's transl)]. 134 salmonella strains, isolated from animals in 1955-65 were compared to 133 salmonella strains isolated in 1973-74, with regard to their drug resistance and the frequentcy of R-factors. No significant changes were observed during the reference period. Salmonella typhi-murium in comparison with other serotypes harboured a significantly larger number of resistant strains. Of the recently isolated resistant strains that were searched for R-factors, about half the number were carriers. All strains were sensitive to chloramphenicole and neomycin."}
{"id": "PMID:1098013", "title": "[Prolactin adenoma. Study of gonadotrophic function by the sassoles G LHRH test].", "content": "An intravenous injection of synthetic LHRH (50mug) was given in 10 patients with prolactin secreting pituitary adenomas. Variations in circulating levels of gonadotrophic hormones were measured by radioimmunological estimation. The increase in blood level of luteinizing hormone was constant, low in three cases, normal in 3 and high in 4. An increase in follicle stimulating hormone (FSH) was absent in only two cases. The data obtained indicates the absence of any characteristic value of the LHRH test in the aetiological diagnosis of amenorrhoea/galactorrhoea syndromes. They represent an argument in favour of the relative character and of the functional nature of the gonadotrophic insufficiency of prolactin adenomas.", "contents": "[Prolactin adenoma. Study of gonadotrophic function by the sassoles G LHRH test]. An intravenous injection of synthetic LHRH (50mug) was given in 10 patients with prolactin secreting pituitary adenomas. Variations in circulating levels of gonadotrophic hormones were measured by radioimmunological estimation. The increase in blood level of luteinizing hormone was constant, low in three cases, normal in 3 and high in 4. An increase in follicle stimulating hormone (FSH) was absent in only two cases. The data obtained indicates the absence of any characteristic value of the LHRH test in the aetiological diagnosis of amenorrhoea/galactorrhoea syndromes. They represent an argument in favour of the relative character and of the functional nature of the gonadotrophic insufficiency of prolactin adenomas."}
{"id": "PMID:1098015", "title": "[Active immunotherapy of acute leukemia and leukemic lymphosarcoma. Results of 10 years. Study of 200 cases].", "content": "The authors report a ten year study of active immunotherapy using BCG and irradiated allogeneic leukaemic cells in 200 patients. In acute lymphatic leukaemia, 57 out of 168 patients treated in this way remained in primary remission for 18 months to 10 years after active immunotherapy was begun, the relapse rate became low after 18 months and nil after 36 months. The results varied according to prognostic factors: the cytological type, active immunotherapy being above all effective in small cell (microlymphoblastic and prolymphocytic) types with a hope of cure in 50 to 60 p.cent of cases; malignant cellular volume; meningeal deposits. In microlymphoblastic forms the possibility of survival at the 5th year is greater than 90 p.cent. After relapse during active immunotherapy sensitivity to chemotherapy does not seem to be diminished. Trials of active immunotherapy in acute myeloid leukaemia are worthy of further pursuit. The results of active immunotherapy in leukaemic lymphosarcoma show that immunotherapy may be effective in preventing local recurrence, both of tumour as well as in the marrow. Four patients are in apparently complete remission for more than four years. On the basis of these results, trials of active immunotherapy for \"residual disease\" should be undertaken in the field of cancerology, going beyond the realm of leukaemias.", "contents": "[Active immunotherapy of acute leukemia and leukemic lymphosarcoma. Results of 10 years. Study of 200 cases]. The authors report a ten year study of active immunotherapy using BCG and irradiated allogeneic leukaemic cells in 200 patients. In acute lymphatic leukaemia, 57 out of 168 patients treated in this way remained in primary remission for 18 months to 10 years after active immunotherapy was begun, the relapse rate became low after 18 months and nil after 36 months. The results varied according to prognostic factors: the cytological type, active immunotherapy being above all effective in small cell (microlymphoblastic and prolymphocytic) types with a hope of cure in 50 to 60 p.cent of cases; malignant cellular volume; meningeal deposits. In microlymphoblastic forms the possibility of survival at the 5th year is greater than 90 p.cent. After relapse during active immunotherapy sensitivity to chemotherapy does not seem to be diminished. Trials of active immunotherapy in acute myeloid leukaemia are worthy of further pursuit. The results of active immunotherapy in leukaemic lymphosarcoma show that immunotherapy may be effective in preventing local recurrence, both of tumour as well as in the marrow. Four patients are in apparently complete remission for more than four years. On the basis of these results, trials of active immunotherapy for \"residual disease\" should be undertaken in the field of cancerology, going beyond the realm of leukaemias."}
{"id": "PMID:1098018", "title": "[Evaluation of an analgesic activity in man: thermodolorimetery].", "content": "The authors propose an experimental technique which makes possible the evaluation in man of the activity of analgesic or anti-inflammatory medications. The principle of thermodolorimetre threshold of painful thermic sensitivity (S.S.T.D. in the french text) which is stable over a period of time in a given individual receiving a placebo but which varies in a significant fashion in groups treated with a medication belonging to one of the two classifications studied.", "contents": "[Evaluation of an analgesic activity in man: thermodolorimetery]. The authors propose an experimental technique which makes possible the evaluation in man of the activity of analgesic or anti-inflammatory medications. The principle of thermodolorimetre threshold of painful thermic sensitivity (S.S.T.D. in the french text) which is stable over a period of time in a given individual receiving a placebo but which varies in a significant fashion in groups treated with a medication belonging to one of the two classifications studied."}
{"id": "PMID:1098021", "title": "The binding of polyamines and of ethidium bromide to tRNA.", "content": "The binding of spermidine and ethidium bromide to mixed tRNA and phenylalanine tRNA has been studied under equilibrium conditions. The numbers and classes of binding sites obtained have been compared to those found in complexes isolated by gel filtration a low ionic strength. The latter complexes contain 10-11 moles of either spermidine or ethidium per mole of tRNA; either cation is completely displaceable by the other. In ethidium complexes, the first 2-3 moles are bound in fluorescent binding sites; the remaining 7-8 molecules bind in non-fluorescent form. At least one of the binding sites for spermidine appears similar to a binding site for fluorescent ethidium. Similar results are found with E. coli formylmethionine tRNA. Spermine, in excess of 18-20 moles per mole tRNA, causes precipitation of the complex. Putrescine does not form isolable complexes with yeast tRNA and displaces ethidium less readily from preformed ethidium-tRNA complexes. Under equilibrium conditions, in the absence of Mg++, there are 16-17 moles of spermidine bound per mole of tRNA as determined by equilibrium dialysis. Of these, 2-3 bind with a Ksence of 9 mM Mg++, the total number of binding sites is decreased slightly and there appears to be only one class of sites with a Ka = 600 M(-1). Quantitatively similar results are obtained for the binding of spermidine to yeast phenylalanine tRNA. When the interaction between ethidium bromide and mixed tRNA is studied by equilibrium dialysis or spectrophotometric titration, two classes of binding sites are obtained: 2-3 molecules bind with an average Ka = 6.6 x 10(5) M(-1) and 14-15 molecules bind with an average Ka = 4.1 x 10(4) M(-1). Spermidine, spermine, and Mg++ compete effectively for both classes of ethidium sites and have the effect of reducing the apparent binding constants for ethidium. When the binding of ethidium is studied by fluorometry, there are 3-4 highly fluorescent sites per tRNA. These sites are also affected by spermidine, spermine and Mg++. Putrescine has little effect on any of the classes of binding sites. These data are consistent with those found under non-equilibrium conditions. They suggest that polyamines bind to fairly specific regions of tRNA and may be involved in the maintenance of certain structural features of tRNA.", "contents": "The binding of polyamines and of ethidium bromide to tRNA. The binding of spermidine and ethidium bromide to mixed tRNA and phenylalanine tRNA has been studied under equilibrium conditions. The numbers and classes of binding sites obtained have been compared to those found in complexes isolated by gel filtration a low ionic strength. The latter complexes contain 10-11 moles of either spermidine or ethidium per mole of tRNA; either cation is completely displaceable by the other. In ethidium complexes, the first 2-3 moles are bound in fluorescent binding sites; the remaining 7-8 molecules bind in non-fluorescent form. At least one of the binding sites for spermidine appears similar to a binding site for fluorescent ethidium. Similar results are found with E. coli formylmethionine tRNA. Spermine, in excess of 18-20 moles per mole tRNA, causes precipitation of the complex. Putrescine does not form isolable complexes with yeast tRNA and displaces ethidium less readily from preformed ethidium-tRNA complexes. Under equilibrium conditions, in the absence of Mg++, there are 16-17 moles of spermidine bound per mole of tRNA as determined by equilibrium dialysis. Of these, 2-3 bind with a Ksence of 9 mM Mg++, the total number of binding sites is decreased slightly and there appears to be only one class of sites with a Ka = 600 M(-1). Quantitatively similar results are obtained for the binding of spermidine to yeast phenylalanine tRNA. When the interaction between ethidium bromide and mixed tRNA is studied by equilibrium dialysis or spectrophotometric titration, two classes of binding sites are obtained: 2-3 molecules bind with an average Ka = 6.6 x 10(5) M(-1) and 14-15 molecules bind with an average Ka = 4.1 x 10(4) M(-1). Spermidine, spermine, and Mg++ compete effectively for both classes of ethidium sites and have the effect of reducing the apparent binding constants for ethidium. When the binding of ethidium is studied by fluorometry, there are 3-4 highly fluorescent sites per tRNA. These sites are also affected by spermidine, spermine and Mg++. Putrescine has little effect on any of the classes of binding sites. These data are consistent with those found under non-equilibrium conditions. They suggest that polyamines bind to fairly specific regions of tRNA and may be involved in the maintenance of certain structural features of tRNA."}
{"id": "PMID:1098022", "title": "Inhibitors of protein synthesis V. Irreversible interaction of antibiotics with an initiation complex.", "content": "The initiation complex (t-complex) formed in a cell-free system (E. coli) from Ac-Phe-tRNA, poly(U) and washed ribosomes in the presence of initiation factors (ribosomal wash) and GTP, contains the Ac-Phe-tRNA bound quantitatively in a puromycin-reactive state. The t-complex is irreversibly inactivated by spiramycin with respect to its reactivity toward puromycin. The inactivated t-complex retains all of the Ac-Phe-tRNA bound, but it does not react with puromycin (2 x10-minus-3M) within 32 min at 25 degrees. In the case of another inhibitor protein synthesis, sparsomycin, the permanently \"modified\" t-complex not only retains all the bound Ac-Phe-tRNA but it can still react with puromycin. In the continuous presence of sparsomycin (1 x 10-minus-7M) the bound Ac-Phe-tRNA reacts quantitatively at a rate which is one-tenth the rate at which the t-complex reacts with puromycin, at low (6.25 x 10-minus-5M) or high (2 x 10-minus-3M) concentrations. These results are not in agreement with current views according to which aparsomycin binds to the ribosome reversibly at a single site with a KI in the range of 10-minus6-10-minus-7 M and according to which this stie is at the A'-site (puromycin site) of peptidyl transferase.", "contents": "Inhibitors of protein synthesis V. Irreversible interaction of antibiotics with an initiation complex. The initiation complex (t-complex) formed in a cell-free system (E. coli) from Ac-Phe-tRNA, poly(U) and washed ribosomes in the presence of initiation factors (ribosomal wash) and GTP, contains the Ac-Phe-tRNA bound quantitatively in a puromycin-reactive state. The t-complex is irreversibly inactivated by spiramycin with respect to its reactivity toward puromycin. The inactivated t-complex retains all of the Ac-Phe-tRNA bound, but it does not react with puromycin (2 x10-minus-3M) within 32 min at 25 degrees. In the case of another inhibitor protein synthesis, sparsomycin, the permanently \"modified\" t-complex not only retains all the bound Ac-Phe-tRNA but it can still react with puromycin. In the continuous presence of sparsomycin (1 x 10-minus-7M) the bound Ac-Phe-tRNA reacts quantitatively at a rate which is one-tenth the rate at which the t-complex reacts with puromycin, at low (6.25 x 10-minus-5M) or high (2 x 10-minus-3M) concentrations. These results are not in agreement with current views according to which aparsomycin binds to the ribosome reversibly at a single site with a KI in the range of 10-minus6-10-minus-7 M and according to which this stie is at the A'-site (puromycin site) of peptidyl transferase."}
{"id": "PMID:1098023", "title": "The metabolism of N4-hydroxycytidine-a mutagen for Salmonella typhimurium.", "content": "Salmonella typhimurium cells were grown in the presence of (14C)-N4-hydroxycytidine (N4OHcyd), a mutagenic nucleoside, and labelling in DNA and RNA digest was traced. The results show that this analogue is incorporated into RNA at a level of 40 mug/100 mg, and into DNA with a yield at least 100 times smaller. Some N-minus4OHcyd was rapidly metabolized and labelling was found in all ribo- and deoxyribo-nucleosides.", "contents": "The metabolism of N4-hydroxycytidine-a mutagen for Salmonella typhimurium. Salmonella typhimurium cells were grown in the presence of (14C)-N4-hydroxycytidine (N4OHcyd), a mutagenic nucleoside, and labelling in DNA and RNA digest was traced. The results show that this analogue is incorporated into RNA at a level of 40 mug/100 mg, and into DNA with a yield at least 100 times smaller. Some N-minus4OHcyd was rapidly metabolized and labelling was found in all ribo- and deoxyribo-nucleosides."}
{"id": "PMID:1098024", "title": "The binding sites for tRNA on eukaryotic ribosomes.", "content": "We have studied the non-enzymic binding of phe-tRNA to ribosomes from rat liver using deacylated tRNA to inhibit binding to the P-site and puromycin (5 x 10-minus3M) to inhibit binding to the A-site. We conclude that at a low concentration of magnesium ions (10mM) phe-tRNA is bound only at the A-site of 80S irbosomes, whereas at a high concentration of magnesium ions (40mM) phe-tRNA is also bound at the P-site. Studies with edeine indicate that, during non-enzymic binding of phe-tRNA, eukaryotic ribosomes (in contrast to prokarotic ribosomes) have the A-site of the 60S subunit and the initiation site of the 40S subunit juxtaposed. This may account for the differences observed, in formation of diphenylalanyl-tRNA and phenylalanyl-puromycin, between phe-tRNA bound non-enzymically to the P-sites of eukaryotic and prokaryotic ribosomes.", "contents": "The binding sites for tRNA on eukaryotic ribosomes. We have studied the non-enzymic binding of phe-tRNA to ribosomes from rat liver using deacylated tRNA to inhibit binding to the P-site and puromycin (5 x 10-minus3M) to inhibit binding to the A-site. We conclude that at a low concentration of magnesium ions (10mM) phe-tRNA is bound only at the A-site of 80S irbosomes, whereas at a high concentration of magnesium ions (40mM) phe-tRNA is also bound at the P-site. Studies with edeine indicate that, during non-enzymic binding of phe-tRNA, eukaryotic ribosomes (in contrast to prokarotic ribosomes) have the A-site of the 60S subunit and the initiation site of the 40S subunit juxtaposed. This may account for the differences observed, in formation of diphenylalanyl-tRNA and phenylalanyl-puromycin, between phe-tRNA bound non-enzymically to the P-sites of eukaryotic and prokaryotic ribosomes."}
{"id": "PMID:1098031", "title": "Blood parameters of dwarf and normal pullets from growth selected lines before and after Escherichia coli challenge.", "content": "Two experiments were conducted to study the hematological differences and the effect of challenge with Escherichia coli on the hemopoietic system among dwarfs and their normal sisters in lines selected for high and low juvenile body weight. Erythrocyte counts, packed cell volumes, and hemoglobin concentrations were significantly higher to dwarf than for normal pullets, while no such differences were noted for mean corpuscular volume, mean corpuscular hemoglobin and hemoglobin concentration. Dwarfs had a significantly lower erythrocyte sedimentation rate than normal pullets. The effect of challenge with E. coli was evident in all the groups. Body weight decreased and leucocyte counts increased significantly. Lymphopenia, accompanied by heterophilia and a consequent increase in H:L ratio was the conspicuous effect of the challenge with E. coli indicating a polymorphonuclear response. Forty-eight hours after challenge the differential response of lines and genotypes to heterophilia was uniform. The hematological data suggest that the dw gene may not contribute to resistance to bacterial diseases. Mortality and autopsy lesions data indicate that the dw gene had a deleterious effect in the low weight but not in the high weight line, suggesting that its influence on the traits measured is dependent upon the genetic background of the population.", "contents": "Blood parameters of dwarf and normal pullets from growth selected lines before and after Escherichia coli challenge. Two experiments were conducted to study the hematological differences and the effect of challenge with Escherichia coli on the hemopoietic system among dwarfs and their normal sisters in lines selected for high and low juvenile body weight. Erythrocyte counts, packed cell volumes, and hemoglobin concentrations were significantly higher to dwarf than for normal pullets, while no such differences were noted for mean corpuscular volume, mean corpuscular hemoglobin and hemoglobin concentration. Dwarfs had a significantly lower erythrocyte sedimentation rate than normal pullets. The effect of challenge with E. coli was evident in all the groups. Body weight decreased and leucocyte counts increased significantly. Lymphopenia, accompanied by heterophilia and a consequent increase in H:L ratio was the conspicuous effect of the challenge with E. coli indicating a polymorphonuclear response. Forty-eight hours after challenge the differential response of lines and genotypes to heterophilia was uniform. The hematological data suggest that the dw gene may not contribute to resistance to bacterial diseases. Mortality and autopsy lesions data indicate that the dw gene had a deleterious effect in the low weight but not in the high weight line, suggesting that its influence on the traits measured is dependent upon the genetic background of the population."}
{"id": "PMID:1098032", "title": "Hemagglutinating properties of Salmonella typhimurium strains isolated from avian sources.", "content": "A total of 565 strains of Salmonella typhimurium and S. typhimurium var. copenhagen, isolated from avian species, were examined for their ability to cause hemagglutination of turkey erythrocytes. Results showed that 541 (95.7%) of the strains caused varying degrees of hemagglutination. The hemagglutinating principle was maintained in cultures stored at 5 degrees C. as formalized suspensions for as long as 6 months. It was destroyed by exposure to a temperature of 80 degrees C. for 1 minute or to absolute ethyl alcohol at 37 degrees C. for 24 hours. The hemagglutinating principle was best preserved in cultures by infrequent transfers, growth in liquid media, lyophilization or storage at low temperatures. Addition of d-mannose to bacteria-erythrocyte mixtures at a final concentration of 0.5% completely inhibited the hemagglutinating activity of the cultures. A concentration of 1% d-mannose added to crystal-violet-stained antigen preparations of S. typhimurium eliminated entirely the hemagglutinating effect of positive cultures and did not interfere with regular agglutination reactions. The hemagglutinating activity of S. typhimurium strains is apparently due to the presence of rigid appendage (fimgriae) on the surface of the bacterial cells. Results from these studies showed that fimbriated cultures are quite common among strains of S. typhimurium.", "contents": "Hemagglutinating properties of Salmonella typhimurium strains isolated from avian sources. A total of 565 strains of Salmonella typhimurium and S. typhimurium var. copenhagen, isolated from avian species, were examined for their ability to cause hemagglutination of turkey erythrocytes. Results showed that 541 (95.7%) of the strains caused varying degrees of hemagglutination. The hemagglutinating principle was maintained in cultures stored at 5 degrees C. as formalized suspensions for as long as 6 months. It was destroyed by exposure to a temperature of 80 degrees C. for 1 minute or to absolute ethyl alcohol at 37 degrees C. for 24 hours. The hemagglutinating principle was best preserved in cultures by infrequent transfers, growth in liquid media, lyophilization or storage at low temperatures. Addition of d-mannose to bacteria-erythrocyte mixtures at a final concentration of 0.5% completely inhibited the hemagglutinating activity of the cultures. A concentration of 1% d-mannose added to crystal-violet-stained antigen preparations of S. typhimurium eliminated entirely the hemagglutinating effect of positive cultures and did not interfere with regular agglutination reactions. The hemagglutinating activity of S. typhimurium strains is apparently due to the presence of rigid appendage (fimgriae) on the surface of the bacterial cells. Results from these studies showed that fimbriated cultures are quite common among strains of S. typhimurium."}
{"id": "PMID:1098033", "title": "The effect of heat exposure and bacterial endotoxin on plasma zinc concentration and the body temperature in the broiler.", "content": "The effect of heat exposure and bacterial endotoxins on plasma zinc levels and mean rectal temperature of 8 week old broilers and investigated. Broilers exposed for 2 hours to 35-35 degrees C. had higher (P less than 0.01) mean rectal temperatures (42.8 vs 41.7 degrees C.) than sham treated broilers maintained at 22-24 degrees C. Likewise, endotoxin (E. coli, 500 mug./kg) treated broilers also had mean rectal temperatures significantly higher (P less than 0.01, 42.4 vs 41.7 degrees C.) than sham treated broilers. Plasma zinc levels in the bacterial endotoxin treated broilers were lower (P less than 0.05) than in the sham treated (173 vs 220, and 219 mug/kg./100 ml. plasma, respectively). In contrast, the plasma zinc concentration was not significantly depressed in the broilers exposed to heat for 2 hours. In summary, either endotoxin or heat exposure resulted in increased body temperature, however only the response to endotoxin resulted in a significant depression of plasma zinc concentration.", "contents": "The effect of heat exposure and bacterial endotoxin on plasma zinc concentration and the body temperature in the broiler. The effect of heat exposure and bacterial endotoxins on plasma zinc levels and mean rectal temperature of 8 week old broilers and investigated. Broilers exposed for 2 hours to 35-35 degrees C. had higher (P less than 0.01) mean rectal temperatures (42.8 vs 41.7 degrees C.) than sham treated broilers maintained at 22-24 degrees C. Likewise, endotoxin (E. coli, 500 mug./kg) treated broilers also had mean rectal temperatures significantly higher (P less than 0.01, 42.4 vs 41.7 degrees C.) than sham treated broilers. Plasma zinc levels in the bacterial endotoxin treated broilers were lower (P less than 0.05) than in the sham treated (173 vs 220, and 219 mug/kg./100 ml. plasma, respectively). In contrast, the plasma zinc concentration was not significantly depressed in the broilers exposed to heat for 2 hours. In summary, either endotoxin or heat exposure resulted in increased body temperature, however only the response to endotoxin resulted in a significant depression of plasma zinc concentration."}
{"id": "PMID:1098037", "title": "Isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques.", "content": "Procedures are described for the separation and purification of cytoplasmic polyhedrosis viruses obtained from multiply infected Heliothis armigera larvae. Separation was achieved by differential centrifugation and density gradient zone electrophoresis followed by complexing with nuclear polyhedrosis virus specific antibody. The yield of cytoplasmic polyhedrosis virus was increased by passage in larvae reared on synthetic media.", "contents": "Isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. Procedures are described for the separation and purification of cytoplasmic polyhedrosis viruses obtained from multiply infected Heliothis armigera larvae. Separation was achieved by differential centrifugation and density gradient zone electrophoresis followed by complexing with nuclear polyhedrosis virus specific antibody. The yield of cytoplasmic polyhedrosis virus was increased by passage in larvae reared on synthetic media."}
{"id": "PMID:1098043", "title": "Growth of mouse megakaryocyte colonies in vitro.", "content": "Mouse bone marrow and spleen cells formed pure or mixed colonies of up to 80 megakaryocytes in agar cultures after stimulation by medium conditioned by activated mouse lymphoid cells. Megakaryocytes were identified on the basis of their morphology, polyploid mitoses and DNA content, and high cytoplasmic content of acetylcholinesterase. Megakaryocyte colony-forming cells were relatively small with a peak sedimentation velocity of 4.2 mm/hr. Spleen, lymph node, and thymus cells produced the factor stimulating megakaryocyte proliferation after culture in medium containing 2-mercaptoethanol, with or without added mitogens or allogeneic spleen cells. Peak activity in conditioning medium was associated with the small lymphocyte fractions in mouse spleen.", "contents": "Growth of mouse megakaryocyte colonies in vitro. Mouse bone marrow and spleen cells formed pure or mixed colonies of up to 80 megakaryocytes in agar cultures after stimulation by medium conditioned by activated mouse lymphoid cells. Megakaryocytes were identified on the basis of their morphology, polyploid mitoses and DNA content, and high cytoplasmic content of acetylcholinesterase. Megakaryocyte colony-forming cells were relatively small with a peak sedimentation velocity of 4.2 mm/hr. Spleen, lymph node, and thymus cells produced the factor stimulating megakaryocyte proliferation after culture in medium containing 2-mercaptoethanol, with or without added mitogens or allogeneic spleen cells. Peak activity in conditioning medium was associated with the small lymphocyte fractions in mouse spleen."}
{"id": "PMID:1098042", "title": "Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations.", "content": "Rho factor has been purified from a strain of E. coli containing the Su78 mutation in the suA gene and assayed in another strain with an amber mutation in the suA gene. The rho from the Su78 mutant strain is present in normal amounts but has altered termination function; it does not terminate transcription at some sites that are recognized effectively by the rho factor from the isogenic wild-type strain. Rho in cells with an amber mutation in the suA gene has been assayed by its RNA-dependent ATPase activity. Extracts of cells of this strain have only 9% as much of this rho activity as extracts of cells of the isogenic wild-type strain. These results suggest that rho is the product of the suA gene. Since mutations in the suA gene are known to decrease polar effects of mutations in other genes, it is also suggested that rho factor is at least partially responsible for polar effects.", "contents": "Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations. Rho factor has been purified from a strain of E. coli containing the Su78 mutation in the suA gene and assayed in another strain with an amber mutation in the suA gene. The rho from the Su78 mutant strain is present in normal amounts but has altered termination function; it does not terminate transcription at some sites that are recognized effectively by the rho factor from the isogenic wild-type strain. Rho in cells with an amber mutation in the suA gene has been assayed by its RNA-dependent ATPase activity. Extracts of cells of this strain have only 9% as much of this rho activity as extracts of cells of the isogenic wild-type strain. These results suggest that rho is the product of the suA gene. Since mutations in the suA gene are known to decrease polar effects of mutations in other genes, it is also suggested that rho factor is at least partially responsible for polar effects."}
{"id": "PMID:1098044", "title": "Determination of genes, restriction sites, and DNA sequences surrounding the 6S RNA template of bacteriophage lambda.", "content": "A major product of the transcription of bacteriophage lambda DNA in vitro is the 6S RNA. This article presents a detailed mapping of restriction endonuclease cleavage sites about the region of the 6S RNA template within the lambda genome. Restriction fragments defined by these sites have been used to localize the 6S RNA template within the physical and genetic maps of the lambda genome. Nucleotide sequence analysis of one of these fragments has largely confimed the nucleotide sequence of the 6S RNA reported previously and has indicated the sequence of DNA that immediately follows the 6S RNA template. This article reports the nucleotide sequence following a known site of transcription termination by RNA polymerase of Escherichia coli.", "contents": "Determination of genes, restriction sites, and DNA sequences surrounding the 6S RNA template of bacteriophage lambda. A major product of the transcription of bacteriophage lambda DNA in vitro is the 6S RNA. This article presents a detailed mapping of restriction endonuclease cleavage sites about the region of the 6S RNA template within the lambda genome. Restriction fragments defined by these sites have been used to localize the 6S RNA template within the physical and genetic maps of the lambda genome. Nucleotide sequence analysis of one of these fragments has largely confimed the nucleotide sequence of the 6S RNA reported previously and has indicated the sequence of DNA that immediately follows the 6S RNA template. This article reports the nucleotide sequence following a known site of transcription termination by RNA polymerase of Escherichia coli."}
{"id": "PMID:1098045", "title": "Single-strand scissions of chromosomal DNA during commitment to recombination at meiosis.", "content": "Diploid cells of the yeast Saccharomyces cerevisiae induced to undergo meiosis accumulate single-strand scissions in both template and newly synthesized DNA during commitment to genetic recombination. No evidence for accumulation of double-strand breaks during meiosis was obtained. When commitment to recombination is at the full meiotic level there are approximately 70 to 200 single-strand scissions per meiotic cell in which approximately 150 recombination events have been reported to occur.", "contents": "Single-strand scissions of chromosomal DNA during commitment to recombination at meiosis. Diploid cells of the yeast Saccharomyces cerevisiae induced to undergo meiosis accumulate single-strand scissions in both template and newly synthesized DNA during commitment to genetic recombination. No evidence for accumulation of double-strand breaks during meiosis was obtained. When commitment to recombination is at the full meiotic level there are approximately 70 to 200 single-strand scissions per meiotic cell in which approximately 150 recombination events have been reported to occur."}
{"id": "PMID:1098046", "title": "Changing patterns of actin localization during cell division.", "content": "Changing patterns of actin localization have been studied on a light microscopic level by means of fluorescently labeled heavy meromyosin. The cellular distribution of actin is characterized by four major patterns, each of which corresponds to a particular phase of cell division. Long actin fibers are a prominent feature of the interphase cell. They disappear as the cell rounds up for mitosis and are replaced by a diffuse distribution of actin throughout the cytoplasm. During cytokinesis, the actin is localized predominantly in the cleavage furrow. The final shift of actin occurs after the completion of cytokinesis. At this time the actin becomes concentrated in the distal poles of the cell where pseudopods form to pull the daughter cells apart. When the daughter cells have separated, they flatten on the culture dish and the fibrous pattern of actin characteristic of interphase cells returns. All of these changes take place during the 1-hr period required for cell division.", "contents": "Changing patterns of actin localization during cell division. Changing patterns of actin localization have been studied on a light microscopic level by means of fluorescently labeled heavy meromyosin. The cellular distribution of actin is characterized by four major patterns, each of which corresponds to a particular phase of cell division. Long actin fibers are a prominent feature of the interphase cell. They disappear as the cell rounds up for mitosis and are replaced by a diffuse distribution of actin throughout the cytoplasm. During cytokinesis, the actin is localized predominantly in the cleavage furrow. The final shift of actin occurs after the completion of cytokinesis. At this time the actin becomes concentrated in the distal poles of the cell where pseudopods form to pull the daughter cells apart. When the daughter cells have separated, they flatten on the culture dish and the fibrous pattern of actin characteristic of interphase cells returns. All of these changes take place during the 1-hr period required for cell division."}
{"id": "PMID:1098047", "title": "Presence of two polypeptide chains comprising fatty acid synthetase.", "content": "Highly purified fatty acid synthetases of chicken and rat livers have molecular weights of 500,000 and dissociate in solutions of low ionic strength into subunits of molecular weight 250,000 with loss of synthetase activity. The subunits can be reassociated in phosphate buffer with full restoration of the activity. In the presence of sodium dodecyl sulfate or guanifine-HCl, the synthetases dissociate into polypeptide chains of molecular weight 220,000 as determined by sodium dodecyl sulfate-gel electrophoresis and sedimentation equilibrium. The polypeptide contains the 4-phosphopantetheine group and the [14C]acetyl and [4C]malonyl groups if the synthetases were prelabeled with [14C]acetyl-CoA and [14C]malonyl-CoA. Similar results were obtained with the synthetase from yeast, except the subunit has a molecular weight of 200,000. These observations indicate that the multi-catalytic activities of the synthetases and the acyl carrier protein are associated only with the two polypeptide chains. The findings suggest a novel structural organization for multienzyme complexes.", "contents": "Presence of two polypeptide chains comprising fatty acid synthetase. Highly purified fatty acid synthetases of chicken and rat livers have molecular weights of 500,000 and dissociate in solutions of low ionic strength into subunits of molecular weight 250,000 with loss of synthetase activity. The subunits can be reassociated in phosphate buffer with full restoration of the activity. In the presence of sodium dodecyl sulfate or guanifine-HCl, the synthetases dissociate into polypeptide chains of molecular weight 220,000 as determined by sodium dodecyl sulfate-gel electrophoresis and sedimentation equilibrium. The polypeptide contains the 4-phosphopantetheine group and the [14C]acetyl and [4C]malonyl groups if the synthetases were prelabeled with [14C]acetyl-CoA and [14C]malonyl-CoA. Similar results were obtained with the synthetase from yeast, except the subunit has a molecular weight of 200,000. These observations indicate that the multi-catalytic activities of the synthetases and the acyl carrier protein are associated only with the two polypeptide chains. The findings suggest a novel structural organization for multienzyme complexes."}
{"id": "PMID:1098049", "title": "Insulin secretion by perfused islets from the obese Zucker rat.", "content": "The dynamics of insulin secretion from pancreatic islets of the Zucker-obese rat were studied by in vitro perfusion of individual islets. Glucose and L-leucine were used as insulinogenic stimuli. Control pancreatic islets were obtained from both normal weight Zucker-thin littermates and equivalent weight Sprague-Dawley rats. Our results demonstrate that pancreatic islets from 13-wk-old Zucker-obese rats hypersecrete insulin in the basal state and in response to both glucose and amino acid (L-leucine) stimuli. Neither pancreatic islets from control Zucker-thin littermate animals (matched with the Zucker-obese animals for age or for total body weight), nor islets from Sprague-Dawley rats of comparable age and weight demonstrate comparable hypersecretion of insulin. These findings; i.e., hypersecretion of insulin from obese pancreatic islets, suggest that the plasma hyperinsulinism characteristic of the obese state is maintained, at least in part, by an inherent abnormality of beta cell secretion. Whether this abnormality in beta cell secretion results from a genetic trait in the Zucker-obese strain or is induced by the insulin resistance of the obese animal is not resolved by this study. In any case, the observed in vitro hyperinsulin secretion from these islets supports the postulation that in vivo peripheral insulin resistance characteristic of obesity may be a physiological response that protects the animal from insulin-induced hypoglycemia.", "contents": "Insulin secretion by perfused islets from the obese Zucker rat. The dynamics of insulin secretion from pancreatic islets of the Zucker-obese rat were studied by in vitro perfusion of individual islets. Glucose and L-leucine were used as insulinogenic stimuli. Control pancreatic islets were obtained from both normal weight Zucker-thin littermates and equivalent weight Sprague-Dawley rats. Our results demonstrate that pancreatic islets from 13-wk-old Zucker-obese rats hypersecrete insulin in the basal state and in response to both glucose and amino acid (L-leucine) stimuli. Neither pancreatic islets from control Zucker-thin littermate animals (matched with the Zucker-obese animals for age or for total body weight), nor islets from Sprague-Dawley rats of comparable age and weight demonstrate comparable hypersecretion of insulin. These findings; i.e., hypersecretion of insulin from obese pancreatic islets, suggest that the plasma hyperinsulinism characteristic of the obese state is maintained, at least in part, by an inherent abnormality of beta cell secretion. Whether this abnormality in beta cell secretion results from a genetic trait in the Zucker-obese strain or is induced by the insulin resistance of the obese animal is not resolved by this study. In any case, the observed in vitro hyperinsulin secretion from these islets supports the postulation that in vivo peripheral insulin resistance characteristic of obesity may be a physiological response that protects the animal from insulin-induced hypoglycemia."}
{"id": "PMID:1098050", "title": "Stereologic evaluation of granular pneumocyte lamellar bodies in different species.", "content": "Lamellar bodies in individual pulmonary glanular pneumocytes in the species examined (mouse, rat, rabbit, and dog) have virtually the same volume density with respect to the cytoplasmic volume as estimated by stereological techniques. The surface-to-volume ratio for these structures also fail to show any interspecies variation in these species.", "contents": "Stereologic evaluation of granular pneumocyte lamellar bodies in different species. Lamellar bodies in individual pulmonary glanular pneumocytes in the species examined (mouse, rat, rabbit, and dog) have virtually the same volume density with respect to the cytoplasmic volume as estimated by stereological techniques. The surface-to-volume ratio for these structures also fail to show any interspecies variation in these species."}
{"id": "PMID:1098048", "title": "Proposed mechanism of bacteriophage lambda induction: acquisition of binding sites for lambda repressor by DNA of the host.", "content": "Interference with the in vitro binding of lambda phage repressor to lambda operator DNA was observed when Escherichia coli DNA containing the following lesions was present in the reaction mixture: (a) DNA with single-strand breaks from pancreatic DNase (nicked DNA); (B) DNA isolated from thymine-straved cells; (c) DNA from ultraviolet-treated cells; (d) DNA of mitomycin-treated cells; and (e) DNA from a temperature-sensitive ligase mutant after 1 hr at 42 degrees. Normal E. coli DNA did not interfere. Binding of lambda cIing-minus repressor to operator DNA was not affected by E. coli DNA with lesions. DNAs from cells treated with increasing doses of mitomycin were proportionately more effective in competition for repressor, suggesting increasing binding sites per unit of DNA. A general model of virus induction is proposed, based on binding affinity of ultraviolet-sensitive repressors for single-strand breaks in the host DNA. The model is extended also to the presumptive repressor of cell division.", "contents": "Proposed mechanism of bacteriophage lambda induction: acquisition of binding sites for lambda repressor by DNA of the host. Interference with the in vitro binding of lambda phage repressor to lambda operator DNA was observed when Escherichia coli DNA containing the following lesions was present in the reaction mixture: (a) DNA with single-strand breaks from pancreatic DNase (nicked DNA); (B) DNA isolated from thymine-straved cells; (c) DNA from ultraviolet-treated cells; (d) DNA of mitomycin-treated cells; and (e) DNA from a temperature-sensitive ligase mutant after 1 hr at 42 degrees. Normal E. coli DNA did not interfere. Binding of lambda cIing-minus repressor to operator DNA was not affected by E. coli DNA with lesions. DNAs from cells treated with increasing doses of mitomycin were proportionately more effective in competition for repressor, suggesting increasing binding sites per unit of DNA. A general model of virus induction is proposed, based on binding affinity of ultraviolet-sensitive repressors for single-strand breaks in the host DNA. The model is extended also to the presumptive repressor of cell division."}
{"id": "PMID:1098051", "title": "Effect of native levan on homograft rejection in mice.", "content": "The effect of high-molecular-weight levan on skin graft rejection was studied. Daily ip administration of 15-30 mg levan was shown to delay rejection in Balb/c and C57BL recipient mice. An increase in MST value by 3.6 days was obtained in Balb/c mice and of 5.6 days for C57BL.", "contents": "Effect of native levan on homograft rejection in mice. The effect of high-molecular-weight levan on skin graft rejection was studied. Daily ip administration of 15-30 mg levan was shown to delay rejection in Balb/c and C57BL recipient mice. An increase in MST value by 3.6 days was obtained in Balb/c mice and of 5.6 days for C57BL."}
{"id": "PMID:1098053", "title": "Long-term culture of isolated pancreatic islet cells.", "content": "Successful long-term culture of isolated pancreatic islet as well as dissociated islet cells was accomplished with the use of Microtest II trays which provides the very small amount of culture medium that is easily conditioned. In the culture of isolated islet, the ultrastructure of the islet cells was completely preserved up to the 30th day and vigorous secretion of insulin was maintained even on the 75th day. While in the islet cell culture, insulin secretion gradually decreased from the 12th day. The latter procedure makes inoculation of a definite number of dissociated pure islet cells possible, thus opeing the way to the quantitative evaluation of B cell function in vitro.", "contents": "Long-term culture of isolated pancreatic islet cells. Successful long-term culture of isolated pancreatic islet as well as dissociated islet cells was accomplished with the use of Microtest II trays which provides the very small amount of culture medium that is easily conditioned. In the culture of isolated islet, the ultrastructure of the islet cells was completely preserved up to the 30th day and vigorous secretion of insulin was maintained even on the 75th day. While in the islet cell culture, insulin secretion gradually decreased from the 12th day. The latter procedure makes inoculation of a definite number of dissociated pure islet cells possible, thus opeing the way to the quantitative evaluation of B cell function in vitro."}
{"id": "PMID:1098054", "title": "Lysosomal enzyme secretion and volume contraction induced in neutrophils by cytochalasin B, chemotactic factor and A23187.", "content": "The secretion of lysosomal enzymes from rabbit peritoneal polymorphonuclear leukocytes caused by either cytochalasin B, or cytochalasin B plus chemotactic factors, or by the divalent ionophore, A23187, is associated with a decrease in the volume of these same cells. No quantitative correlation could be found between the extent of lysosomal enzyme secretion and volume decrease for any of the three stimuli. This suggests that the volume decrease is neither the direct cause nor the result of the secretion.", "contents": "Lysosomal enzyme secretion and volume contraction induced in neutrophils by cytochalasin B, chemotactic factor and A23187. The secretion of lysosomal enzymes from rabbit peritoneal polymorphonuclear leukocytes caused by either cytochalasin B, or cytochalasin B plus chemotactic factors, or by the divalent ionophore, A23187, is associated with a decrease in the volume of these same cells. No quantitative correlation could be found between the extent of lysosomal enzyme secretion and volume decrease for any of the three stimuli. This suggests that the volume decrease is neither the direct cause nor the result of the secretion."}
{"id": "PMID:1098055", "title": "Similar vasoconstrictor responses to calcium in normotensive and esssential hypertensive men.", "content": "To study limb vascular responses in man to elevations in plasma calcium concentrations, we infused test isosmolar solutions of CaCl2 (0.115, 0.230, and 0.460 meq calcium/min) and NaCl and control isosmolar solutions of NaCl into the brachial arteries of 10 normotensive men and eight men with essential hypertension of mild to moderate severity. Limb blood pressures were monitored, limb blood flow was measured by indicator-dilution, and limb vascular resistance was calculated as mm Hg/ml flow/min/100 cm3 limb volume. Measured concentration of calcium in limb venous plasma during infusion of 0.460 meq calcium/min was 11.5 +/- 0.8 meq/liter (mean +/- SEM) with individual values ranging up to 20 meq/liter. Changes in limb venous serum sodium, potassium, magnesium, and osmolality were similar during control and CaCl2 infusions. Decreases in limb venous blood hematocrit during CaCl2 infusions were the same or greater than those during control infusions. The infusions did not significantly change systemic blood calcium concentration or blood pressures. Limb blood flow decreased and resistance increased in response to CaCl2. Increments averaging as little as 2.2 meq/liter elevated limb resistance by about 45%. Log dose-response curves were linear. Responses did not differ in normotensives and hypertensives (P greater than 0.8). We conclude that the vascular response to acute elevation of plasma calcium concentrations up to 20 meq/liter in the limb oman is an impressive vasoconstriction. We found no evidence for abnormal vascular responses to calcium in essential hypertensive men.", "contents": "Similar vasoconstrictor responses to calcium in normotensive and esssential hypertensive men. To study limb vascular responses in man to elevations in plasma calcium concentrations, we infused test isosmolar solutions of CaCl2 (0.115, 0.230, and 0.460 meq calcium/min) and NaCl and control isosmolar solutions of NaCl into the brachial arteries of 10 normotensive men and eight men with essential hypertension of mild to moderate severity. Limb blood pressures were monitored, limb blood flow was measured by indicator-dilution, and limb vascular resistance was calculated as mm Hg/ml flow/min/100 cm3 limb volume. Measured concentration of calcium in limb venous plasma during infusion of 0.460 meq calcium/min was 11.5 +/- 0.8 meq/liter (mean +/- SEM) with individual values ranging up to 20 meq/liter. Changes in limb venous serum sodium, potassium, magnesium, and osmolality were similar during control and CaCl2 infusions. Decreases in limb venous blood hematocrit during CaCl2 infusions were the same or greater than those during control infusions. The infusions did not significantly change systemic blood calcium concentration or blood pressures. Limb blood flow decreased and resistance increased in response to CaCl2. Increments averaging as little as 2.2 meq/liter elevated limb resistance by about 45%. Log dose-response curves were linear. Responses did not differ in normotensives and hypertensives (P greater than 0.8). We conclude that the vascular response to acute elevation of plasma calcium concentrations up to 20 meq/liter in the limb oman is an impressive vasoconstriction. We found no evidence for abnormal vascular responses to calcium in essential hypertensive men."}
{"id": "PMID:1098061", "title": "Effects of stress and drugs on the catecholamine and pepsin concentrations in stomach wall of rat.", "content": "Reserpine, serotonin, and histamine as well as stress were employed to produced dystrophic lesions in the gastric mucosa of rats. Analysis of stomach tissues revealed that agencies which reduced catecholamine concentration also decreased endogenous levels of pepsinogen. Concentrations of the zymogen were increased after parenteral administration of dopa, but were not changed by injections of alpha-methyl-dopa. The evidence supports the suggestion that catecholamines participate in the control of pepsin production and are involved in the secretory function of the stomach.", "contents": "Effects of stress and drugs on the catecholamine and pepsin concentrations in stomach wall of rat. Reserpine, serotonin, and histamine as well as stress were employed to produced dystrophic lesions in the gastric mucosa of rats. Analysis of stomach tissues revealed that agencies which reduced catecholamine concentration also decreased endogenous levels of pepsinogen. Concentrations of the zymogen were increased after parenteral administration of dopa, but were not changed by injections of alpha-methyl-dopa. The evidence supports the suggestion that catecholamines participate in the control of pepsin production and are involved in the secretory function of the stomach."}
{"id": "PMID:1098063", "title": "Infrared scattering: a method for evaluating the mass and size of bacteria.", "content": "In the realm of biophysics and biochemistry, light scattering is used extensively to evaluate the molecular weight and size of macromolecules and particles in suspension. Bacteria scatter strongly but are too large for the conventional procedures. By extending the wavelength lambda of the incident radiation into the infrared, we show that the effective size of the bacteria (relative to lambda) is reduced, and the usual Zimm plot measurements and procedure can be applied to evaluate the molecular weight and size. Details of the apparatus, its alignment, calibration and use are given along with data for aqueous suspensions of the three species Serratia marcescens, Escherichia coli and Thiobacillus ferrooxidans. The method has the advantages of being suitable for rod-like or ellipsoid-like bacteria as well as spheres, for polydisperse samples and for monitoring the effects of environment, antibodies and chemical therapeutic agents on the bacteria.", "contents": "Infrared scattering: a method for evaluating the mass and size of bacteria. In the realm of biophysics and biochemistry, light scattering is used extensively to evaluate the molecular weight and size of macromolecules and particles in suspension. Bacteria scatter strongly but are too large for the conventional procedures. By extending the wavelength lambda of the incident radiation into the infrared, we show that the effective size of the bacteria (relative to lambda) is reduced, and the usual Zimm plot measurements and procedure can be applied to evaluate the molecular weight and size. Details of the apparatus, its alignment, calibration and use are given along with data for aqueous suspensions of the three species Serratia marcescens, Escherichia coli and Thiobacillus ferrooxidans. The method has the advantages of being suitable for rod-like or ellipsoid-like bacteria as well as spheres, for polydisperse samples and for monitoring the effects of environment, antibodies and chemical therapeutic agents on the bacteria."}
{"id": "PMID:1098058", "title": "Effects of 6-hydroxydopamine on shock-elicited aggression, emotionality and maternal behavior in female rats.", "content": "Tests of emotionality and shock-elicited aggression, which have revealed consistent changes in behavior in male rats given 6-hydroxydopamine (6-OHDA), were administered to female rats intraventricularly injected with 250 mug of 6-OHDA to determine whether aspects of the 6-OHDA syndrome are shared by female and male rats. The results confirmed that female rats become hyperemotional as well as hyperaggressive after 6-OHDA, as do males. Using the hypothesis that the behavioral effects of 6-OHDA lesions of catecholamine neurons result from a general reduction in threshold to aversive stimuli, it was predicted that arbitrarily selected stimuli having aversive components should enhance the behavior normally elicited by these stimuli. Stimuli selected for testing were those that normally elicit maternal defensive aggression and maternal retrieval. Both of these behaviors were found to be enhanced in 6-OHDA treated female rats, thus supporting the prediction. These findings were interpreted as inconsistent with the hypothesis that norepinephrine chemically codes specific behaviors, particularly aggressive behavior.", "contents": "Effects of 6-hydroxydopamine on shock-elicited aggression, emotionality and maternal behavior in female rats. Tests of emotionality and shock-elicited aggression, which have revealed consistent changes in behavior in male rats given 6-hydroxydopamine (6-OHDA), were administered to female rats intraventricularly injected with 250 mug of 6-OHDA to determine whether aspects of the 6-OHDA syndrome are shared by female and male rats. The results confirmed that female rats become hyperemotional as well as hyperaggressive after 6-OHDA, as do males. Using the hypothesis that the behavioral effects of 6-OHDA lesions of catecholamine neurons result from a general reduction in threshold to aversive stimuli, it was predicted that arbitrarily selected stimuli having aversive components should enhance the behavior normally elicited by these stimuli. Stimuli selected for testing were those that normally elicit maternal defensive aggression and maternal retrieval. Both of these behaviors were found to be enhanced in 6-OHDA treated female rats, thus supporting the prediction. These findings were interpreted as inconsistent with the hypothesis that norepinephrine chemically codes specific behaviors, particularly aggressive behavior."}
{"id": "PMID:1098065", "title": "A 24-hour periodicity in the activity of a nickel-coated glass slide used for carrying out immunologic reactions at a liquid-solid interface.", "content": "Active nickel-plated slides are prepared by evaporating the metal in the presence of a magnetic field oriented perpendicularly to the slide, the surface being metallized facing the south pole of the field. Active slides coated with an antigen layer (bovine albumin or ovalbumin) can specifically adsorb a layer of antibodies nearly twice as thick (70 to 80 A) as that adsorbed on inactive slides (40 A). The activity of a slide varies within a 24-hour period. Fully active at night, a slide gradually loses its activity from sunrise on but regains it at sunset. The experimental evidence favors the view that inactivation is brought about by soft cosmic rays emanating from the sun. The inactivation can be prevented by keeping the slide under 3.5 cm of lead.", "contents": "A 24-hour periodicity in the activity of a nickel-coated glass slide used for carrying out immunologic reactions at a liquid-solid interface. Active nickel-plated slides are prepared by evaporating the metal in the presence of a magnetic field oriented perpendicularly to the slide, the surface being metallized facing the south pole of the field. Active slides coated with an antigen layer (bovine albumin or ovalbumin) can specifically adsorb a layer of antibodies nearly twice as thick (70 to 80 A) as that adsorbed on inactive slides (40 A). The activity of a slide varies within a 24-hour period. Fully active at night, a slide gradually loses its activity from sunrise on but regains it at sunset. The experimental evidence favors the view that inactivation is brought about by soft cosmic rays emanating from the sun. The inactivation can be prevented by keeping the slide under 3.5 cm of lead."}
{"id": "PMID:1098066", "title": "The effect of sodium bisulfite on the melting profiles of nucleic acids and on their respective nucleosides.", "content": "The effect of sodium bisulfite (0.27 M, pH 7) on melting behavior of DNA, yeast RNA and their respective nucleosides was studied. It was found that bisulfite added not only to pyrimidine bases but also to purine bases of nucleic acids and of nucleosides. The addition products were stable at higher temperatures but reverted to parent compounds at room temperature. The only exception was the addition product of uridine which was stable at room temperature and could be isolated by paper chromatography in a 42-62% yield. Heating of DNA solutions in the presence of bisulfite to 95 degrees C caused a 90% loss of absorbance at 260 nm. On cooling, the absorbance was essentially recovered. When compared to the melting behavior of DNA in 0.27 M NaCl or 0.09 M Na2SO4 (same ionic strength), it was found that bisulfite destabilized double helical structure of DNA and that reversible addition of bisulfite did occur much below the melting temperature of DNA observed in the other two solvents.", "contents": "The effect of sodium bisulfite on the melting profiles of nucleic acids and on their respective nucleosides. The effect of sodium bisulfite (0.27 M, pH 7) on melting behavior of DNA, yeast RNA and their respective nucleosides was studied. It was found that bisulfite added not only to pyrimidine bases but also to purine bases of nucleic acids and of nucleosides. The addition products were stable at higher temperatures but reverted to parent compounds at room temperature. The only exception was the addition product of uridine which was stable at room temperature and could be isolated by paper chromatography in a 42-62% yield. Heating of DNA solutions in the presence of bisulfite to 95 degrees C caused a 90% loss of absorbance at 260 nm. On cooling, the absorbance was essentially recovered. When compared to the melting behavior of DNA in 0.27 M NaCl or 0.09 M Na2SO4 (same ionic strength), it was found that bisulfite destabilized double helical structure of DNA and that reversible addition of bisulfite did occur much below the melting temperature of DNA observed in the other two solvents."}
{"id": "PMID:1098068", "title": "Radiographic approach to stereotaxic surgery in rhesus monkeys.", "content": "A method is described which uses radiographic techniques to identify ventricular and bony landmarks as guides in placing probes into specific areas of the brain in the rhesus monkey. This approach requires a relatively simple modification of a standard stereotaxic instrument and is applicable to various physiological and behavioral experiments in species where cranial variability limits the accuracy of stereotaxic surgery.", "contents": "Radiographic approach to stereotaxic surgery in rhesus monkeys. A method is described which uses radiographic techniques to identify ventricular and bony landmarks as guides in placing probes into specific areas of the brain in the rhesus monkey. This approach requires a relatively simple modification of a standard stereotaxic instrument and is applicable to various physiological and behavioral experiments in species where cranial variability limits the accuracy of stereotaxic surgery."}
{"id": "PMID:1098069", "title": "The anatomy of several free flap donor sites.", "content": "The vascular anatomy of the supplying vessels to the iliofemoral, the deltopectoral, the axillary, and the thoracoabdominal regions has been investigated in detail in fresh cadavers. Other donor sites were also examined. The anatomical relationship of the supplying vessels is discussed-and the design, conversion, and utilization of these skin areas as donor sites for free flap transfer are detailed. The iliofemoral region is suggested as the best donor site for most free flap procedures.", "contents": "The anatomy of several free flap donor sites. The vascular anatomy of the supplying vessels to the iliofemoral, the deltopectoral, the axillary, and the thoracoabdominal regions has been investigated in detail in fresh cadavers. Other donor sites were also examined. The anatomical relationship of the supplying vessels is discussed-and the design, conversion, and utilization of these skin areas as donor sites for free flap transfer are detailed. The iliofemoral region is suggested as the best donor site for most free flap procedures."}
{"id": "PMID:1098070", "title": "Laser, scalpel, electrosurgical, and tangential excisions of third degree burns. A preliminary report.", "content": "Our clinical experience with laser, electrosurgical, scalpel, and tangential burn wound excisions in 26 patients is presented.", "contents": "Laser, scalpel, electrosurgical, and tangential excisions of third degree burns. A preliminary report. Our clinical experience with laser, electrosurgical, scalpel, and tangential burn wound excisions in 26 patients is presented."}
{"id": "PMID:1098075", "title": "One-stage coverage of the large chest wall defect with a giant bepedicled flap. Case reports.", "content": "We present a method of closing chest wall defects with a large bepedicled flap from the adjacent chest wall and abdominal tissue.", "contents": "One-stage coverage of the large chest wall defect with a giant bepedicled flap. Case reports. We present a method of closing chest wall defects with a large bepedicled flap from the adjacent chest wall and abdominal tissue."}
{"id": "PMID:1098076", "title": "A method to keep dressings moist prior to delayed skin grafting.", "content": "A simple method is described of using a commercial drape or sheet of polyethylene to keep dressings moist prior to the application of delayed skin grafts.", "contents": "A method to keep dressings moist prior to delayed skin grafting. A simple method is described of using a commercial drape or sheet of polyethylene to keep dressings moist prior to the application of delayed skin grafts."}
{"id": "PMID:1098084", "title": "Nomifensine: a potent dopaminergic agonist of antiparkinson potential.", "content": "Nomifensine was shown to be a potent stereotypic agent in rat. Its effect was resistant to a-methylparatyrosine pretreatment but was abolished by combined reserpine/alpha-methylparatyrosine and by haloperidol. Electrolytic lesions placed in dopamine-containing areas of the extra-pyramidal, mesolimbic and amygdaloid systems indicated an effect in all areas, but the globus pallidus and substantianigra were shown to be most important for its action. Also the effect of nomifensine was reduced by lesions of the medial and/or dorsal raph\u00e9 nuclei. A circling behaviour was recorded following nomifensine administration to animals with unilateral electrolytic lesions of the substantia nigra or asymmetric lesions of the medial raph\u00e9 nucleus. These effects were resistant to alpha-methylparatyrosine and inhibited by haloperidol. Nomifensine reduced the intensity of harmine-induced tremor. The M2-metabolic of nomifensine mimicked the effects of the parent compound on peripheral administration bu the onset of action was more rapid and  the duration shorter. The M2-metabolite was active on intrastriatal injection to induce stereotyped/hyperactive behaviour and contralateral asymmetries. In all experimental situations nomifensine was compared with apomorphine and d-amphetamine (dopamine and L-Dopa where appropriate). Nomifensine/metabolite was shown to be a potent dopaminergic agonist with an action mainly dependent upon functioning of the extrapyramidal system and partly independent of presynaptic mechanisms.", "contents": "Nomifensine: a potent dopaminergic agonist of antiparkinson potential. Nomifensine was shown to be a potent stereotypic agent in rat. Its effect was resistant to a-methylparatyrosine pretreatment but was abolished by combined reserpine/alpha-methylparatyrosine and by haloperidol. Electrolytic lesions placed in dopamine-containing areas of the extra-pyramidal, mesolimbic and amygdaloid systems indicated an effect in all areas, but the globus pallidus and substantianigra were shown to be most important for its action. Also the effect of nomifensine was reduced by lesions of the medial and/or dorsal raph\u00e9 nuclei. A circling behaviour was recorded following nomifensine administration to animals with unilateral electrolytic lesions of the substantia nigra or asymmetric lesions of the medial raph\u00e9 nucleus. These effects were resistant to alpha-methylparatyrosine and inhibited by haloperidol. Nomifensine reduced the intensity of harmine-induced tremor. The M2-metabolic of nomifensine mimicked the effects of the parent compound on peripheral administration bu the onset of action was more rapid and  the duration shorter. The M2-metabolite was active on intrastriatal injection to induce stereotyped/hyperactive behaviour and contralateral asymmetries. In all experimental situations nomifensine was compared with apomorphine and d-amphetamine (dopamine and L-Dopa where appropriate). Nomifensine/metabolite was shown to be a potent dopaminergic agonist with an action mainly dependent upon functioning of the extrapyramidal system and partly independent of presynaptic mechanisms."}
{"id": "PMID:1098085", "title": "N-desmethyldiazepam and amylobarbitone sodium as hypnotics in anxious patients. Plasma levels, clinical efficacy and residual effects.", "content": "Drug plasma levels of N-desmethyldiazepam (10 and 20 mg/day given at night) and amylobarbitone sodium (200 mg/day given at night) were determined in 45 patients after 1 and 7 days of treatment together with estimates of hypnotic and residual effects. No relationships were found between clinical rati ngs of hypnotic effect and drug plasma levels, while significant correlations were observed between some performance taska and drug plasma concentrations of amylobarbitone sodium, and of the higher dose of N-desmethyldiazepam.", "contents": "N-desmethyldiazepam and amylobarbitone sodium as hypnotics in anxious patients. Plasma levels, clinical efficacy and residual effects. Drug plasma levels of N-desmethyldiazepam (10 and 20 mg/day given at night) and amylobarbitone sodium (200 mg/day given at night) were determined in 45 patients after 1 and 7 days of treatment together with estimates of hypnotic and residual effects. No relationships were found between clinical rati ngs of hypnotic effect and drug plasma levels, while significant correlations were observed between some performance taska and drug plasma concentrations of amylobarbitone sodium, and of the higher dose of N-desmethyldiazepam."}
{"id": "PMID:1098086", "title": "Inhibition of d-amphetamine-induced locomotor activity by injection of haloperidol into the nucleus accumbens of the rat.", "content": "The effect of intracerebral administraton of antagonists of dopamine and noradrenaline upon the locomotor stimulation induced by intraperitoneal injection of d-amphetamine sulfate in rats was investigated. Inj ection of low doses of the dopamine antagonist haloperidol (2.5 mug and 5 mug) bilaterally into the nucleus accumbens antagonized the locomotor stimulation following d-amphetamine. No significant inhibition was observed following administration of the alpha-adrenergic antagonist phentolamine or thhe beta-adrenergic antagonist propranolol into the nucleus accumbens. Injection of the dame doses of haloperidol into the caudate nucleus did not inhibit the d-amphetamine induced locomotor activity in contrast to the effects seen following injection into the nucleus accumbens. The results confirm the significance of dopaminergic mechanisms for the locomotor stimulant effect of d-amphetamine and indicate that the mesolimbic dopamine system plays an important role in this respect.", "contents": "Inhibition of d-amphetamine-induced locomotor activity by injection of haloperidol into the nucleus accumbens of the rat. The effect of intracerebral administraton of antagonists of dopamine and noradrenaline upon the locomotor stimulation induced by intraperitoneal injection of d-amphetamine sulfate in rats was investigated. Inj ection of low doses of the dopamine antagonist haloperidol (2.5 mug and 5 mug) bilaterally into the nucleus accumbens antagonized the locomotor stimulation following d-amphetamine. No significant inhibition was observed following administration of the alpha-adrenergic antagonist phentolamine or thhe beta-adrenergic antagonist propranolol into the nucleus accumbens. Injection of the dame doses of haloperidol into the caudate nucleus did not inhibit the d-amphetamine induced locomotor activity in contrast to the effects seen following injection into the nucleus accumbens. The results confirm the significance of dopaminergic mechanisms for the locomotor stimulant effect of d-amphetamine and indicate that the mesolimbic dopamine system plays an important role in this respect."}
{"id": "PMID:1098087", "title": "Changes in morphine self-administration and morphine dependence after lesions of the caudate nucleus in rats.", "content": "Rats were trained to bar press for intravenous infusions of morphine sulfate during 1-hr daily test sessions.  Small, centrally placed bilateral lesions of the caudate nucleus reduced rats of morphine self-administration to approximately one seventh of preoperative levels; postoperative rates were similar to preoperative rates when the postoperative unit infusion dose of morphine was one tenth of the preoperative dose.  Caudate lesions also lowered the threshold dose at which morphine's rewarding property could be detected.  Physical dependence was studied in other rats receiving a 3-day continuous infusion of morphine sulfate via implanted subcutaneous silicone reservoirs.  Caudate lesions ameliorated withdrawal-induced weight loss and naloxone-induced \"wet dog shakes\".  Both the self-administration and dependence data are consistent with the idea that morphine blocks dopaminergic transmission in the striatum.", "contents": "Changes in morphine self-administration and morphine dependence after lesions of the caudate nucleus in rats. Rats were trained to bar press for intravenous infusions of morphine sulfate during 1-hr daily test sessions.  Small, centrally placed bilateral lesions of the caudate nucleus reduced rats of morphine self-administration to approximately one seventh of preoperative levels; postoperative rates were similar to preoperative rates when the postoperative unit infusion dose of morphine was one tenth of the preoperative dose.  Caudate lesions also lowered the threshold dose at which morphine's rewarding property could be detected.  Physical dependence was studied in other rats receiving a 3-day continuous infusion of morphine sulfate via implanted subcutaneous silicone reservoirs.  Caudate lesions ameliorated withdrawal-induced weight loss and naloxone-induced \"wet dog shakes\".  Both the self-administration and dependence data are consistent with the idea that morphine blocks dopaminergic transmission in the striatum."}
{"id": "PMID:1098088", "title": "The effect of amitriptyline, desipramine and imipramine on the vivo brain synthesis of 3H-noradrenaline from 3H-L-dopa in the rat.", "content": "3H-L-Dopa was given to rats after a peripheral decarboxylase inhibitor Ro 4-4602 (50 mg/kg) and the effect of 30 min pretreatment with antiptyline (10 mg/kg), desipramine (10 mg/kg) and imipramine (10 mg/kg) on the brain formation of 3H-dopamine, 3H-noradrenaline and their major metabolites was investigated. Desipramine produced a decrease in the level of labelled noradrenaline and its major metabolites free and conjugated 3-methoxy-4-hydroxyphenyleneglycol and 3,4-dihydroxyphenyleneglycol. Imipramine decreased labelled noradrenaline and 3-methoxy-4-hydroxyphenyleneglycol, whereas amitriptyline produced no significant effect on noradrenaline metabolism. The thymoleptic drugs produced no significant effect on endogenous brain noradrenaline and dopamine. These findings provide a strong indication that desipramine and imipramine inhibit the 3H-noradrenaline biosynthesis from 3H-L-Dopamthe effect seems closely related to the well-known membrane inhibitory effect of these drugs, since desipramine produced a more marked effect than imipramine and amitriptyline showed no effect. No conclusive evidence for the precise mechanism of action was obtained but it is possible that the decreased 3H-noradrenaline synthesis is related to interference of desipramine and imipramine with the precursor (s) 3H-L-Dopa or 3H-dopamine at sites of 3H-noradrenaline biosynthesis.", "contents": "The effect of amitriptyline, desipramine and imipramine on the vivo brain synthesis of 3H-noradrenaline from 3H-L-dopa in the rat. 3H-L-Dopa was given to rats after a peripheral decarboxylase inhibitor Ro 4-4602 (50 mg/kg) and the effect of 30 min pretreatment with antiptyline (10 mg/kg), desipramine (10 mg/kg) and imipramine (10 mg/kg) on the brain formation of 3H-dopamine, 3H-noradrenaline and their major metabolites was investigated. Desipramine produced a decrease in the level of labelled noradrenaline and its major metabolites free and conjugated 3-methoxy-4-hydroxyphenyleneglycol and 3,4-dihydroxyphenyleneglycol. Imipramine decreased labelled noradrenaline and 3-methoxy-4-hydroxyphenyleneglycol, whereas amitriptyline produced no significant effect on noradrenaline metabolism. The thymoleptic drugs produced no significant effect on endogenous brain noradrenaline and dopamine. These findings provide a strong indication that desipramine and imipramine inhibit the 3H-noradrenaline biosynthesis from 3H-L-Dopamthe effect seems closely related to the well-known membrane inhibitory effect of these drugs, since desipramine produced a more marked effect than imipramine and amitriptyline showed no effect. No conclusive evidence for the precise mechanism of action was obtained but it is possible that the decreased 3H-noradrenaline synthesis is related to interference of desipramine and imipramine with the precursor (s) 3H-L-Dopa or 3H-dopamine at sites of 3H-noradrenaline biosynthesis."}
{"id": "PMID:1098090", "title": "Erythroid toxicity of azathioprin. Macrocytosis and selective marrow hypoplasis.", "content": "In a population of 70 stable renal allograft recipients receiving azathioprine, macrocytosis was present in a majority and its severity was related to azathioprine dosage. There were associated megaloblastoid marrow changes which could not be related to serum folate or vitamin B12 concentrations. Two cases of selective red cell hypoplasia in renal allograft recipients are described; in each patient, azathioprine was incriminated and the effect dose-related. These observations indicate that the erythroid effects of azathioprine may precede, and be independent of, its effects on the myeloid series.", "contents": "Erythroid toxicity of azathioprin. Macrocytosis and selective marrow hypoplasis. In a population of 70 stable renal allograft recipients receiving azathioprine, macrocytosis was present in a majority and its severity was related to azathioprine dosage. There were associated megaloblastoid marrow changes which could not be related to serum folate or vitamin B12 concentrations. Two cases of selective red cell hypoplasia in renal allograft recipients are described; in each patient, azathioprine was incriminated and the effect dose-related. These observations indicate that the erythroid effects of azathioprine may precede, and be independent of, its effects on the myeloid series."}
{"id": "PMID:1098091", "title": "Acute reversible renal failure in patients with acute cholecystitis and cholangitis.", "content": "Although acute renal failure is a well recognized complication of several extra-hepatic biliary tract diseases especially biliary tract surgery in the presence of obstructive jaundice, there is little information concerning renal failure in acute cholecystitis. Renal function was assessed in 14 patients with acute cholecystitis and two with acute cholangitis. Six patients had no evidence of renal impairment, four had modest elevations of plasma urea and creatinine concentrations and six had acute reversible renal failure of whom three required peritoneal dialysis. Only one patient was hypovolaemic and in the remainder there was evidence that intravasular coagulation was responsible for the renal failure. It is suggested that bacteraemia was the initiating factor. The therapeutic implications of these findings are discussed.", "contents": "Acute reversible renal failure in patients with acute cholecystitis and cholangitis. Although acute renal failure is a well recognized complication of several extra-hepatic biliary tract diseases especially biliary tract surgery in the presence of obstructive jaundice, there is little information concerning renal failure in acute cholecystitis. Renal function was assessed in 14 patients with acute cholecystitis and two with acute cholangitis. Six patients had no evidence of renal impairment, four had modest elevations of plasma urea and creatinine concentrations and six had acute reversible renal failure of whom three required peritoneal dialysis. Only one patient was hypovolaemic and in the remainder there was evidence that intravasular coagulation was responsible for the renal failure. It is suggested that bacteraemia was the initiating factor. The therapeutic implications of these findings are discussed."}
{"id": "PMID:1098100", "title": "Radiology of the urinary tract: some physiological considerations. Annual oration in memory of John S. Bouslog, M.D., 1890-1973.", "content": "This paper reviews some of the fundamental technical and diagnostic problems in clinical uroradiology, such as (a) the reason for the obstructive nephrogram; (b) why high-dose urography gives better studies than low-dose; (c) why the Trueta phenomenon has not been demonstrated in man; (d) the case for an intrarenal arterial collateral pathway via the pericalyceal network (these vessels can be demonstrated in man and must not be misinterpreted as tumor vessels or vascular malformations). One of the most baffling problems sometimes occurs with the sudden partial block of a single segmental vessel: the kidney ceases to excrete urine and eventually becomes atrophic even though some excretory function returns. To date, there is no satisfactory explanation for this course of events.", "contents": "Radiology of the urinary tract: some physiological considerations. Annual oration in memory of John S. Bouslog, M.D., 1890-1973. This paper reviews some of the fundamental technical and diagnostic problems in clinical uroradiology, such as (a) the reason for the obstructive nephrogram; (b) why high-dose urography gives better studies than low-dose; (c) why the Trueta phenomenon has not been demonstrated in man; (d) the case for an intrarenal arterial collateral pathway via the pericalyceal network (these vessels can be demonstrated in man and must not be misinterpreted as tumor vessels or vascular malformations). One of the most baffling problems sometimes occurs with the sudden partial block of a single segmental vessel: the kidney ceases to excrete urine and eventually becomes atrophic even though some excretory function returns. To date, there is no satisfactory explanation for this course of events."}
{"id": "PMID:1098101", "title": "Arteriography of renal transplants.", "content": "Arteriograms were reviewed in 60 cases of renal transplant dysfunction and correlated with the final diagnosis. Angiographic refinements included selective injections, 2-4X direct magnification, and flow-dependent injection rates. Angiography permitted recognition of common causes of post-transplantation dysfunction, including acute vasomotor nephropathy (AVN), acute refection (AR), chronic rejection, and obstruction of the ureter, renal artery, or renal vein. In 28 patients with AVN or AR who had technically adequate cortical microangiograms, classification was correct in 57%, indeterminate in 36%, and erroneous in 7%. In addition to its diagnostic value, angiography provides some prognostic information in AR, permitting prediction of functional return when the pattern suggests AVN and lack of return when cortical necrosis is indicated.", "contents": "Arteriography of renal transplants. Arteriograms were reviewed in 60 cases of renal transplant dysfunction and correlated with the final diagnosis. Angiographic refinements included selective injections, 2-4X direct magnification, and flow-dependent injection rates. Angiography permitted recognition of common causes of post-transplantation dysfunction, including acute vasomotor nephropathy (AVN), acute refection (AR), chronic rejection, and obstruction of the ureter, renal artery, or renal vein. In 28 patients with AVN or AR who had technically adequate cortical microangiograms, classification was correct in 57%, indeterminate in 36%, and erroneous in 7%. In addition to its diagnostic value, angiography provides some prognostic information in AR, permitting prediction of functional return when the pattern suggests AVN and lack of return when cortical necrosis is indicated."}
{"id": "PMID:1098102", "title": "The potential of localized heating as a adjunct to radiation therapy.", "content": "Experimental studies have shown that (a) tumor cells may be more sensitive to heat than normal cells; (b) hyperthermia inactivates cellular repair mechanisms for radiation damage; and (c) heat may lower the OER for ionizing radiation (anoxic cells are at least as sensitive to hyperthermia as oxygenated cells). Localized hyperthemia produced by localized current fields in the range of 100 kHz-10 MHz by direct contact electrodes offers two major advantages: the eletrode configurations may be manipulated to obtain desired thermal dose distributions, and, since the mode of heating is essentially instantaneous, accurate temperature control can be maintained during treatment.", "contents": "The potential of localized heating as a adjunct to radiation therapy. Experimental studies have shown that (a) tumor cells may be more sensitive to heat than normal cells; (b) hyperthermia inactivates cellular repair mechanisms for radiation damage; and (c) heat may lower the OER for ionizing radiation (anoxic cells are at least as sensitive to hyperthermia as oxygenated cells). Localized hyperthemia produced by localized current fields in the range of 100 kHz-10 MHz by direct contact electrodes offers two major advantages: the eletrode configurations may be manipulated to obtain desired thermal dose distributions, and, since the mode of heating is essentially instantaneous, accurate temperature control can be maintained during treatment."}
{"id": "PMID:1098106", "title": "The impact of computed tomography on neuroradiologic practice.", "content": "To assess the impact of computed tomography (CT) of the head on neuroradiologic and diagnostic practice, we analyzed its capabilities, performance, and usage in our practice during the past 18 months. The results were compared to similar data on other neurodiagnostic tests, i.e., electroencephalography (EEG), A-mode echoencephalography, cerebral angiography, cerebral pneumography, and nuclide brain scanning. All diagnostic techniques except EEG were used less often as the capabilities of CT became known to our clinicians.", "contents": "The impact of computed tomography on neuroradiologic practice. To assess the impact of computed tomography (CT) of the head on neuroradiologic and diagnostic practice, we analyzed its capabilities, performance, and usage in our practice during the past 18 months. The results were compared to similar data on other neurodiagnostic tests, i.e., electroencephalography (EEG), A-mode echoencephalography, cerebral angiography, cerebral pneumography, and nuclide brain scanning. All diagnostic techniques except EEG were used less often as the capabilities of CT became known to our clinicians."}
{"id": "PMID:1098107", "title": "An approach to contrast enhancement in computed tomography of the brain.", "content": "A total of 450 brain examinations were performed using computed tomography before and after intravenous infusion of methylglucamine diatrizoate 30%. In 110 cases involving a documented intracranial lesion, the scans were analyzed to determine the degree and usefulness of contrast enhancement. Enhancement was achieved in a wide variety of neoplastic and non-neoplastic lesions and was found to be of considerable help in detection of abnormality as well as in defining tumor morphology.", "contents": "An approach to contrast enhancement in computed tomography of the brain. A total of 450 brain examinations were performed using computed tomography before and after intravenous infusion of methylglucamine diatrizoate 30%. In 110 cases involving a documented intracranial lesion, the scans were analyzed to determine the degree and usefulness of contrast enhancement. Enhancement was achieved in a wide variety of neoplastic and non-neoplastic lesions and was found to be of considerable help in detection of abnormality as well as in defining tumor morphology."}
{"id": "PMID:1098108", "title": "Evaluation of mammographic calcifications using a computer program.", "content": "A computer pattern recognition program was developed to evaluate calcifications seen on mammograms. When the approximate horizontal length, average internal gray level, and contrast were used as criteria, 84.3% of the lesions studied were correctly identified as malignant or benign. These results show that a computer program can help evaluate calcifications of the breast.", "contents": "Evaluation of mammographic calcifications using a computer program. A computer pattern recognition program was developed to evaluate calcifications seen on mammograms. When the approximate horizontal length, average internal gray level, and contrast were used as criteria, 84.3% of the lesions studied were correctly identified as malignant or benign. These results show that a computer program can help evaluate calcifications of the breast."}
{"id": "PMID:1098109", "title": "Sealed sources in synthetic absorbable suture: a new method for permanent interstitial implantation.", "content": "Inactive gold grains are embedded at 1-cm intervals along a synthetic, absorbable surgical suture, then subjected to neutron irradiation in a nuclear reactor, forming a new material for permanent interstitial implant therapy. Assembling and activating the sealed sources in an absorbable carrier, the physical properties of the irradiated suture, its behavior in tissue, and the methods and results of implantation in the dog are described.", "contents": "Sealed sources in synthetic absorbable suture: a new method for permanent interstitial implantation. Inactive gold grains are embedded at 1-cm intervals along a synthetic, absorbable surgical suture, then subjected to neutron irradiation in a nuclear reactor, forming a new material for permanent interstitial implant therapy. Assembling and activating the sealed sources in an absorbable carrier, the physical properties of the irradiated suture, its behavior in tissue, and the methods and results of implantation in the dog are described."}
{"id": "PMID:1098113", "title": "Luteolysis, estrus and ovulation, and blood prostaglandin F after intramuscular administration of 15, 30 or 60 mg prostaglanding F2alpha.", "content": "During diestrus in three consecutive estrous cycles, each of six heifers was given (im) 30 mg, 15 mg (twice at 6-hr intervals) and 60 mg prostaglandin F2alpha (PGF2alpha) tham salt. Neither the decline in blood progesterone, the increase in blood estradiol, the duration or the peak of the LH surge, the interval to onset of estrus, nor the interval to ovulation was affected significantly by dose of PGF2alpha. Thus, relative to that after 30 mg PGF2alpha im, two injections of 15 mg at 6-hr intervals or 60 mg PGF2alpha did not hasten luteolysis. Thirty mg was an ample in dose of PGF2alpha to cause luteolysis. Regardless of im dose of PGF2alpha, blood PGF peaked at about 6.0 ng/ml within 10 minutes and returned to basal values (less than 1.0 ng/ml) within 90 minutes. In another trial, after a single iv injection of 5 mg PGF2alpha, blood PGF peaked (25 ng/ml) within 5 minutes and returned to basal values within 15 minutes. During a 30-minute infusion (0.5 mg/minute) of PGF2alpha, blood PGF plateaued at 29.5 ng/ml with a metabolic clearance rate of 17.0 liters per minute.", "contents": "Luteolysis, estrus and ovulation, and blood prostaglandin F after intramuscular administration of 15, 30 or 60 mg prostaglanding F2alpha. During diestrus in three consecutive estrous cycles, each of six heifers was given (im) 30 mg, 15 mg (twice at 6-hr intervals) and 60 mg prostaglandin F2alpha (PGF2alpha) tham salt. Neither the decline in blood progesterone, the increase in blood estradiol, the duration or the peak of the LH surge, the interval to onset of estrus, nor the interval to ovulation was affected significantly by dose of PGF2alpha. Thus, relative to that after 30 mg PGF2alpha im, two injections of 15 mg at 6-hr intervals or 60 mg PGF2alpha did not hasten luteolysis. Thirty mg was an ample in dose of PGF2alpha to cause luteolysis. Regardless of im dose of PGF2alpha, blood PGF peaked at about 6.0 ng/ml within 10 minutes and returned to basal values (less than 1.0 ng/ml) within 90 minutes. In another trial, after a single iv injection of 5 mg PGF2alpha, blood PGF peaked (25 ng/ml) within 5 minutes and returned to basal values within 15 minutes. During a 30-minute infusion (0.5 mg/minute) of PGF2alpha, blood PGF plateaued at 29.5 ng/ml with a metabolic clearance rate of 17.0 liters per minute."}
{"id": "PMID:1098117", "title": "Effect of the antiepileptic drug sodium valproate on induction of hepatic microsomal P450.", "content": "Sodium valproate is an anticonvulsant which, unlike phenobarbitone, does not produce an increase in rat hepatic P450 when given in large nontherapeutic doses.", "contents": "Effect of the antiepileptic drug sodium valproate on induction of hepatic microsomal P450. Sodium valproate is an anticonvulsant which, unlike phenobarbitone, does not produce an increase in rat hepatic P450 when given in large nontherapeutic doses."}
{"id": "PMID:1098118", "title": "Demonstration of methadone in the human brain by immunofluorescence.", "content": "Specific neuronal staining for methadone was demonstrated by immunofluorescence in the brain tissue of ten addicts who had died from drug overdose. Areas of the brain most frequently involved were the hypothalamus, thalamus, hippocampus, amygdala, cerebellum and brain stem. Toxicological studies confirmed the presence of methadone in each case.", "contents": "Demonstration of methadone in the human brain by immunofluorescence. Specific neuronal staining for methadone was demonstrated by immunofluorescence in the brain tissue of ten addicts who had died from drug overdose. Areas of the brain most frequently involved were the hypothalamus, thalamus, hippocampus, amygdala, cerebellum and brain stem. Toxicological studies confirmed the presence of methadone in each case."}
{"id": "PMID:1098119", "title": "Frequency of surface immunoglobulin bearing blood lymphocytes in cattle affected with bovine leukosis.", "content": "Lymphocytes of the peripheral blood from healthy cows and animals suffering from bovine leukosis (persistent lymphocytosis or leukaemia) were examined for the presence of surface immunoglobulin determinants. Using the immunofluorescence technique an average of 18 per cent of lymphocytes from healthy and 67 per cent from leukotic cows could be recognised as positive. An increase of these positive cells, which are supposed to be B cells or of B cell origin, seems to be a characteristic finding in this disease of cattle.", "contents": "Frequency of surface immunoglobulin bearing blood lymphocytes in cattle affected with bovine leukosis. Lymphocytes of the peripheral blood from healthy cows and animals suffering from bovine leukosis (persistent lymphocytosis or leukaemia) were examined for the presence of surface immunoglobulin determinants. Using the immunofluorescence technique an average of 18 per cent of lymphocytes from healthy and 67 per cent from leukotic cows could be recognised as positive. An increase of these positive cells, which are supposed to be B cells or of B cell origin, seems to be a characteristic finding in this disease of cattle."}
{"id": "PMID:1098120", "title": "The direct fluorescent antibody test for the detection of Treponema hyodysenteria in pigs.", "content": "A direct fluorescent antibody technique for the detection of Treponema hyodysenteriae in faeces and bowel smears from pigs produced a better preparation than the indirect method and also had the advantages that it took less time, produced less non-specific fluorescnece and gave direct control over the availability of the reagents used.", "contents": "The direct fluorescent antibody test for the detection of Treponema hyodysenteria in pigs. A direct fluorescent antibody technique for the detection of Treponema hyodysenteriae in faeces and bowel smears from pigs produced a better preparation than the indirect method and also had the advantages that it took less time, produced less non-specific fluorescnece and gave direct control over the availability of the reagents used."}
{"id": "PMID:1098126", "title": "[Dentofacial orthopedics and orthopedic surgery].", "content": "The authors describe five cases of surgical correction of dysmorphisms of the maxillaries requiring orthodontic treatment before surgery. Pre-operative orthodontic treatment of the adult has the advantage of enabling the arches to be aligned, facilitating the operation when the osteotomized fragments are positioned and resulting in immobilisation in a good position.", "contents": "[Dentofacial orthopedics and orthopedic surgery]. The authors describe five cases of surgical correction of dysmorphisms of the maxillaries requiring orthodontic treatment before surgery. Pre-operative orthodontic treatment of the adult has the advantage of enabling the arches to be aligned, facilitating the operation when the osteotomized fragments are positioned and resulting in immobilisation in a good position."}
{"id": "PMID:1098129", "title": "[Note on lingual mutilations and their possible surgical repair].", "content": "A historical review is followed by a case report concerning a 58-year-old man cured of a pelvilingual carcinoma at the price of severe mutilation in whom it was possible, with the aid of cutaneo-cylindircal grafts, to close a buccocervical fistula and to anatomically reconstruct the tongue. This rare case proves that after treatment for carcinoma, stomatological plastic surgery is possible when cure is certain and the patient's general condition good.", "contents": "[Note on lingual mutilations and their possible surgical repair]. A historical review is followed by a case report concerning a 58-year-old man cured of a pelvilingual carcinoma at the price of severe mutilation in whom it was possible, with the aid of cutaneo-cylindircal grafts, to close a buccocervical fistula and to anatomically reconstruct the tongue. This rare case proves that after treatment for carcinoma, stomatological plastic surgery is possible when cure is certain and the patient's general condition good."}
{"id": "PMID:1098130", "title": "Decrease in gastric secretion during the first three months after proximal gastric vagotomy in duodenal ulcer patients.", "content": "Gastric secretion was stimulated by intravenous infusion of 15 mug/kg/hr of pentagastrin before and 10 and 90 days after proximal gastric vagotomy in 16 duodenal ulcer patients. Postoperatively 15 ug/kg/hr of pentagastrin was also given in combination with 2 mug/kg/hr of carbacholine. Mean acid output in response to pentagastrin alone was reduced by 48 and 64 per cent at 10 and 90 days after the vagotomy, respectively. Mean pentagastrin-stimulated acid output and volume of gastric juice was significantly higher at 10 days than at 90 days after the operation. Mean pepsin output decreased also, but the decrease was not statistically significant. There was no significant effect of carbacholine on pentagastrin-stimulated acid output either at 10 or 90 days. Carbacholine increased pentagastrin-stimulated volume of gastric juice significantly at 10 but not 90 days postoperatively. The effect of carbacholine on pentagastrin-stimulated pepsin output was significant both 10 and 90 days after the vagotomy. Pepsin output in response to pentagastrin plus carbacholine 10 days after the operation was not significantly different from preoperative values. At 10 days postoperatively the increase in pentagastrin-stimulated volume of gastric juice by carbacholine was significantly greater than at 90 days. The corresponding differences of acid and pepsin outputs were not significant.", "contents": "Decrease in gastric secretion during the first three months after proximal gastric vagotomy in duodenal ulcer patients. Gastric secretion was stimulated by intravenous infusion of 15 mug/kg/hr of pentagastrin before and 10 and 90 days after proximal gastric vagotomy in 16 duodenal ulcer patients. Postoperatively 15 ug/kg/hr of pentagastrin was also given in combination with 2 mug/kg/hr of carbacholine. Mean acid output in response to pentagastrin alone was reduced by 48 and 64 per cent at 10 and 90 days after the vagotomy, respectively. Mean pentagastrin-stimulated acid output and volume of gastric juice was significantly higher at 10 days than at 90 days after the operation. Mean pepsin output decreased also, but the decrease was not statistically significant. There was no significant effect of carbacholine on pentagastrin-stimulated acid output either at 10 or 90 days. Carbacholine increased pentagastrin-stimulated volume of gastric juice significantly at 10 but not 90 days postoperatively. The effect of carbacholine on pentagastrin-stimulated pepsin output was significant both 10 and 90 days after the vagotomy. Pepsin output in response to pentagastrin plus carbacholine 10 days after the operation was not significantly different from preoperative values. At 10 days postoperatively the increase in pentagastrin-stimulated volume of gastric juice by carbacholine was significantly greater than at 90 days. The corresponding differences of acid and pepsin outputs were not significant."}
{"id": "PMID:1098132", "title": "Role of intravenously infused insulin in treatment of acute pancreatitis. A double-blind study.", "content": "The effect of insulin treatment in acute pancreatitis was studied in a double-blind investigation. Fifteen patients with clinical signs of acute pancreatitis were treated with intravenous infusion of glucose and insulin only. Twelve patients were free from pain within four hours of commencement of treatment. Within eight hours all fifteen were free from abdominal pain. Fourteen patients with pancreatitis established by the same criteria were treated with glucose infusions without insulin. Only four of the patients in this group became free from pain within four hours. The role of intravenously infused insulin with respect to the more beneficial effect in the first group is discussed.", "contents": "Role of intravenously infused insulin in treatment of acute pancreatitis. A double-blind study. The effect of insulin treatment in acute pancreatitis was studied in a double-blind investigation. Fifteen patients with clinical signs of acute pancreatitis were treated with intravenous infusion of glucose and insulin only. Twelve patients were free from pain within four hours of commencement of treatment. Within eight hours all fifteen were free from abdominal pain. Fourteen patients with pancreatitis established by the same criteria were treated with glucose infusions without insulin. Only four of the patients in this group became free from pain within four hours. The role of intravenously infused insulin with respect to the more beneficial effect in the first group is discussed."}
{"id": "PMID:1098133", "title": "Inhibition of leucocyte migration by antigens from human colon and E. coli O 14 in patients with ulcerative colitis.", "content": "In 55 patients with ulcerative colitis and in 25 controls the leucocyte migration inhibition test was performed. As antigens the extract from human fetal colon and the lipopolysaccharide antigen from E. coli O 14 were used. The leucocyte migration in patients with ulcerative colitis was significantly inhibited in the presence of the antigens mentioned. In patients with an active form of ulcerative colitis the leucocyte migration was significantly inhibited as compared to the leucocyte migration in patients with a nonactive form of the disease.", "contents": "Inhibition of leucocyte migration by antigens from human colon and E. coli O 14 in patients with ulcerative colitis. In 55 patients with ulcerative colitis and in 25 controls the leucocyte migration inhibition test was performed. As antigens the extract from human fetal colon and the lipopolysaccharide antigen from E. coli O 14 were used. The leucocyte migration in patients with ulcerative colitis was significantly inhibited in the presence of the antigens mentioned. In patients with an active form of ulcerative colitis the leucocyte migration was significantly inhibited as compared to the leucocyte migration in patients with a nonactive form of the disease."}
{"id": "PMID:1098134", "title": "The effects of acetylsalicylic acid on the human gastric mucosa as revealed by gastrocamera.", "content": "The influence on the gastric mucosa of acetylsalicylic acid (ASA) given as a buffered solution (Bamyl-SR, AB H\u00e4ssle, Sweden) and as a conventional disintegrating tablet (MagnecylR, ACO, Sweden) has been compared in 7 healthy volunteers, using the gastrocamera technique. The study was performed crossover with randomized treatment periods. The dosage of ASA was 1 g 3 times daily for 3 days. Before and after each treatment period gastrocamera examinations were performed. The evaluation of the gastro-camera films was made blindly. It was found that after the ASA-tablets all subjects had multiple and prominent erosions. However, in all subjects the erosive effects on the gastric mucosa were less pronounced with the buffered solution. It was concluded that any person taking conventional ASA-tablets in a dose of 1 g 3 times daily for 3 days runs the risk of developing lesions of the gastric mucosa. This risk is less when ASA is taken in the form of a buffered solution.", "contents": "The effects of acetylsalicylic acid on the human gastric mucosa as revealed by gastrocamera. The influence on the gastric mucosa of acetylsalicylic acid (ASA) given as a buffered solution (Bamyl-SR, AB H\u00e4ssle, Sweden) and as a conventional disintegrating tablet (MagnecylR, ACO, Sweden) has been compared in 7 healthy volunteers, using the gastrocamera technique. The study was performed crossover with randomized treatment periods. The dosage of ASA was 1 g 3 times daily for 3 days. Before and after each treatment period gastrocamera examinations were performed. The evaluation of the gastro-camera films was made blindly. It was found that after the ASA-tablets all subjects had multiple and prominent erosions. However, in all subjects the erosive effects on the gastric mucosa were less pronounced with the buffered solution. It was concluded that any person taking conventional ASA-tablets in a dose of 1 g 3 times daily for 3 days runs the risk of developing lesions of the gastric mucosa. This risk is less when ASA is taken in the form of a buffered solution."}
{"id": "PMID:1098135", "title": "Output of bilirubin and pancreatic enzymes in response to different liquid test meals in man.", "content": "The output of bilirubin and pancreatic enzymes was determined after ingestion of three different liquid test meals in man. Doubling the amount of protein in a composite meal was followed by a delivery of more protein per time unit to the intestine and by a higher output of bilirubin and enzymes than was recorded after a similar meal containing less protein. The ingestion of a 15% glucose solution awoke a considerable output of bilirubin and enzymes during the first hour, thereafter it was low and decreasing. The differences in enzyme and bilirubin output corresponded to differences in quality and quantity of the emptied food components.", "contents": "Output of bilirubin and pancreatic enzymes in response to different liquid test meals in man. The output of bilirubin and pancreatic enzymes was determined after ingestion of three different liquid test meals in man. Doubling the amount of protein in a composite meal was followed by a delivery of more protein per time unit to the intestine and by a higher output of bilirubin and enzymes than was recorded after a similar meal containing less protein. The ingestion of a 15% glucose solution awoke a considerable output of bilirubin and enzymes during the first hour, thereafter it was low and decreasing. The differences in enzyme and bilirubin output corresponded to differences in quality and quantity of the emptied food components."}
{"id": "PMID:1098136", "title": "[The short novel \"The jealous Estremaduran\", an example of Cervantes' psychopathology].", "content": "The Cervantinie short novel \"The Jealous Estremaduran\" describes with poetic liberty the senile jealousy-imagination of a man nearly 80 years old towards his 15-year-old new bride. The preposterous, patholplastic forms of this jealousy-phantasm make the main figure of the short novel, the old man Carrizales, his absurd, fantastie plans of a hermetical isolation of his wife from the outside-world a reality. CERVANTES describes in his novel, without the noxa alcohol playing any part though, a state of affairs which is similar to the symptoms of the chronic jealousy-phantasm of the alcoholic who only has in his mind's eye the sheer wish to possess his partner. These details prescribed in a person with a pathological derangement, which show CERVANTES as an excellent psychopathologist among the poets, are examined and analysed.", "contents": "[The short novel \"The jealous Estremaduran\", an example of Cervantes' psychopathology]. The Cervantinie short novel \"The Jealous Estremaduran\" describes with poetic liberty the senile jealousy-imagination of a man nearly 80 years old towards his 15-year-old new bride. The preposterous, patholplastic forms of this jealousy-phantasm make the main figure of the short novel, the old man Carrizales, his absurd, fantastie plans of a hermetical isolation of his wife from the outside-world a reality. CERVANTES describes in his novel, without the noxa alcohol playing any part though, a state of affairs which is similar to the symptoms of the chronic jealousy-phantasm of the alcoholic who only has in his mind's eye the sheer wish to possess his partner. These details prescribed in a person with a pathological derangement, which show CERVANTES as an excellent psychopathologist among the poets, are examined and analysed."}
{"id": "PMID:1098128", "title": "[Primary cheilorhinoplasty for congenital unilateral labiomaxillary fissure. Trial schematization of a technic].", "content": "The addition of rhinoplasty to primary cheiloplasty for cleft lip improves not only the post-operative state but also, by re-establishing the equilibrium of all the cartilaginous and musculo-periosteal structures, favourises maxillary growth. The author describes a technique and presents some results.", "contents": "[Primary cheilorhinoplasty for congenital unilateral labiomaxillary fissure. Trial schematization of a technic]. The addition of rhinoplasty to primary cheiloplasty for cleft lip improves not only the post-operative state but also, by re-establishing the equilibrium of all the cartilaginous and musculo-periosteal structures, favourises maxillary growth. The author describes a technique and presents some results."}
{"id": "PMID:1098137", "title": "[Electro-clinical evolution of cerebral spongiosis].", "content": "Electroencephalogram was repeated about every third week in the last five months' course of a spongiform encephalopathy, Creutzfeldt-Jakob type. This confirms the interest of EEG and its diagnostic value in certain presenile dementias. The appearance of triphasic stereotyped complexes with a short periodic interval is the fundamental pattern allowing to predict, then to assess, the diagnosis. Our study is based upon 14 polygraphic records (including EEG, respiratory rhythm, ocular movements and limbs' actogram); compared with previous studies from the literature it shows some pecularities: - periodic discharges with an interval of about one second appearing early, i.e. before the clinical picture became typical, -lack of any change under the influence of various drugs and stimulations, - stability of the pattern throughout the whole course of the disease.", "contents": "[Electro-clinical evolution of cerebral spongiosis]. Electroencephalogram was repeated about every third week in the last five months' course of a spongiform encephalopathy, Creutzfeldt-Jakob type. This confirms the interest of EEG and its diagnostic value in certain presenile dementias. The appearance of triphasic stereotyped complexes with a short periodic interval is the fundamental pattern allowing to predict, then to assess, the diagnosis. Our study is based upon 14 polygraphic records (including EEG, respiratory rhythm, ocular movements and limbs' actogram); compared with previous studies from the literature it shows some pecularities: - periodic discharges with an interval of about one second appearing early, i.e. before the clinical picture became typical, -lack of any change under the influence of various drugs and stimulations, - stability of the pattern throughout the whole course of the disease."}
{"id": "PMID:1098138", "title": "[The beginning of neurosurgery].", "content": "Trephining was practiced by neolithic man. But neurosurgery as a speciality arose only during the second half of the 19th century. Its first pioneers were Sir William Mac Ewen, Sir Victor Horsley, Fedor Drause, and Antoine Chipault. The leaders of the second generation were Harvey Cushing, Walter Dandy, Charles Elsberg and Charles Frazier. Mention deserve also Otfried Foerster, Thierry de Martel, Clovis Vincent, Ren\u00e9 Leriche and Ludwig Puusepp.", "contents": "[The beginning of neurosurgery]. Trephining was practiced by neolithic man. But neurosurgery as a speciality arose only during the second half of the 19th century. Its first pioneers were Sir William Mac Ewen, Sir Victor Horsley, Fedor Drause, and Antoine Chipault. The leaders of the second generation were Harvey Cushing, Walter Dandy, Charles Elsberg and Charles Frazier. Mention deserve also Otfried Foerster, Thierry de Martel, Clovis Vincent, Ren\u00e9 Leriche and Ludwig Puusepp."}
{"id": "PMID:1098139", "title": "[Lope de Vegas comedy \"Los locos de Valencia\" a view of a 16th century psychiatric institution].", "content": "With the comedy \"Los locos de Valencia\" (The Insane of Valencia) Lope de Vega left us valuable evidence of the nursing of the insane in the 16th century. After a summarizing presentation of the intricate plot of the play the manifold details about admission, general treatment of the mentally ill as well as the habits in the oldest psychiatric institution of the world are communicated and analysed by means of citations form comedy. Furthermore, an attempt is being made to demonstrate the nosolgic understanding of the Humanist and non-physician Lope de Vega for the psychiatric conditions and way of living from the same source.", "contents": "[Lope de Vegas comedy \"Los locos de Valencia\" a view of a 16th century psychiatric institution]. With the comedy \"Los locos de Valencia\" (The Insane of Valencia) Lope de Vega left us valuable evidence of the nursing of the insane in the 16th century. After a summarizing presentation of the intricate plot of the play the manifold details about admission, general treatment of the mentally ill as well as the habits in the oldest psychiatric institution of the world are communicated and analysed by means of citations form comedy. Furthermore, an attempt is being made to demonstrate the nosolgic understanding of the Humanist and non-physician Lope de Vega for the psychiatric conditions and way of living from the same source."}
{"id": "PMID:1098140", "title": "[Priapism--pathogenesis and therapy].", "content": "Priapism can occur when pathologic stimuli (e.g. lesions of the cervical spinal cord) cause prolonged erection, and when normal stimuli under pathologic circumstances (e.g. sickle cell disease, leukemia) produce prolonged erection. As any priapism eventually leads to permanent impotentia coeundi, immediate therapy is absolutely indicated. All unspecific procedures are useless and merely waste time. The priapism in connection with hematologic diseases must be countered by conservative specific therapy (sickle cell disease by blood transfusions; leukemias by cytostatic drugs). All other forms of priapism must be treated by surgery as soon as possible. The operation of choice today perineal bilateral shunt between the corpus cavernosum and the corpus spongiosum", "contents": "[Priapism--pathogenesis and therapy]. Priapism can occur when pathologic stimuli (e.g. lesions of the cervical spinal cord) cause prolonged erection, and when normal stimuli under pathologic circumstances (e.g. sickle cell disease, leukemia) produce prolonged erection. As any priapism eventually leads to permanent impotentia coeundi, immediate therapy is absolutely indicated. All unspecific procedures are useless and merely waste time. The priapism in connection with hematologic diseases must be countered by conservative specific therapy (sickle cell disease by blood transfusions; leukemias by cytostatic drugs). All other forms of priapism must be treated by surgery as soon as possible. The operation of choice today perineal bilateral shunt between the corpus cavernosum and the corpus spongiosum"}
{"id": "PMID:1098141", "title": "[Osteoplastic hip buttress in the treatment of osteoarthritis of the hip].", "content": "The results of 40 acetabuloplasties of the hip for osteoarthritis have been studied over a follow-up period of 6 months to 7 years. 12 patients have been treated by shelf arthroplasty alone and 26 by the same operation combined with intertrochanteric osteotomy. These operations, which are suitable for use in patients too young for a total hip prosthesis, give very satisfactory results in 80% of the cases, but the patients must be carefully selected. The clinical and radiological criteria on which this choice is based, and the technical peculiarities of the operation, are described.", "contents": "[Osteoplastic hip buttress in the treatment of osteoarthritis of the hip]. The results of 40 acetabuloplasties of the hip for osteoarthritis have been studied over a follow-up period of 6 months to 7 years. 12 patients have been treated by shelf arthroplasty alone and 26 by the same operation combined with intertrochanteric osteotomy. These operations, which are suitable for use in patients too young for a total hip prosthesis, give very satisfactory results in 80% of the cases, but the patients must be carefully selected. The clinical and radiological criteria on which this choice is based, and the technical peculiarities of the operation, are described."}
{"id": "PMID:1098143", "title": "[Dental and mandibular studies on mummies. Contribution of a stomatologist].", "content": "This is the sequence to an investigation on a mummy, 1965. Two further mummies could be examined: the first, a woman about 34 years old, from the 1st to 3rd century, shows similar advanced destruction of the teeth as the mummy investigated in 1965, caused by sand in the hand milled bread flour. This caused an early loss of molars by exaggerated abrasion, exposure of the pulps and periodontal bone loss. There was no evidence of occlusal caries. The second mummy was a 18-year-old young woman, 800-700 b. C. From the inscriptions on the sarcophagus name, family and living circumstances could be found. The same damages were found here, there was only a gradual difference owing to age difference. An interesting special finding was the absence of lip closure and the postmortal damage to the front teeth which may have been caused by the ritual of mouth opening.", "contents": "[Dental and mandibular studies on mummies. Contribution of a stomatologist]. This is the sequence to an investigation on a mummy, 1965. Two further mummies could be examined: the first, a woman about 34 years old, from the 1st to 3rd century, shows similar advanced destruction of the teeth as the mummy investigated in 1965, caused by sand in the hand milled bread flour. This caused an early loss of molars by exaggerated abrasion, exposure of the pulps and periodontal bone loss. There was no evidence of occlusal caries. The second mummy was a 18-year-old young woman, 800-700 b. C. From the inscriptions on the sarcophagus name, family and living circumstances could be found. The same damages were found here, there was only a gradual difference owing to age difference. An interesting special finding was the absence of lip closure and the postmortal damage to the front teeth which may have been caused by the ritual of mouth opening."}
{"id": "PMID:1098144", "title": "Changes in obsessive/compulsive patients as measured by the Leyton Inventory before and after treatment with clomipramine.", "content": "The Leyton Obsessional Inventory has been found to be a useful measure in assessing patients before and after treatment with clomipramine. Mean scores for symptoms and interference altered significantly during the course of treatment. The Leyton Obsessional Inventory, however, lacks external validation owing to the absence of some valid alternative quantification. In the absence of such external validation it seems justifiable to use the mean Leyton score diagnostically but not as a sole indication of severity or response to treatment.", "contents": "Changes in obsessive/compulsive patients as measured by the Leyton Inventory before and after treatment with clomipramine. The Leyton Obsessional Inventory has been found to be a useful measure in assessing patients before and after treatment with clomipramine. Mean scores for symptoms and interference altered significantly during the course of treatment. The Leyton Obsessional Inventory, however, lacks external validation owing to the absence of some valid alternative quantification. In the absence of such external validation it seems justifiable to use the mean Leyton score diagnostically but not as a sole indication of severity or response to treatment."}
{"id": "PMID:1098148", "title": "Flagellar coordination in Chlamydomonas reinhardtii: isolation and reactivation of the flagellar apparatus.", "content": "The flagellar apparatus (both flagella with basal bodies and accessory structures) of Chlamydomonas reinhardtii was isolated from a wall-less mutant and induced to swim in the presence of adenosine triphosphate. The movement of the flagella of the isolated apparatus was largely synchronous and indistinguishable from the movement of flagella on living cells. These observations suggest that the mechanism of flagellar coordination in this biflagellate alga is largely intrinsic to the structure of the flagellar apparatus itself.", "contents": "Flagellar coordination in Chlamydomonas reinhardtii: isolation and reactivation of the flagellar apparatus. The flagellar apparatus (both flagella with basal bodies and accessory structures) of Chlamydomonas reinhardtii was isolated from a wall-less mutant and induced to swim in the presence of adenosine triphosphate. The movement of the flagella of the isolated apparatus was largely synchronous and indistinguishable from the movement of flagella on living cells. These observations suggest that the mechanism of flagellar coordination in this biflagellate alga is largely intrinsic to the structure of the flagellar apparatus itself."}
{"id": "PMID:1098149", "title": "Glucagon release: paradoxical stimulation by glucose during calcium deprivation.", "content": "During calsium deprivation, the rate of glucagon release by the isolated perfused rat pancreas is positively related to the glucose concentration of the perfusion medium. It is suggested that such a paradoxical behavior, which is reminiscent of the abnormality of glucagon secretion recently disclosed in diabetic subjects, results from a perturbation in the normal structural and functional bridging between pancreatic alpha and beta cells.", "contents": "Glucagon release: paradoxical stimulation by glucose during calcium deprivation. During calsium deprivation, the rate of glucagon release by the isolated perfused rat pancreas is positively related to the glucose concentration of the perfusion medium. It is suggested that such a paradoxical behavior, which is reminiscent of the abnormality of glucagon secretion recently disclosed in diabetic subjects, results from a perturbation in the normal structural and functional bridging between pancreatic alpha and beta cells."}
{"id": "PMID:1098152", "title": "Radioligand assay in reproductive biology.", "content": "Radioligand assays have been developed for the principal reproductive steroids and peptide hormones. Specific binding reagents have included antibodies, plasma binders, and intracellular receptors. In each assay, problems of specificity, sensitivity, and nonspecific inhibitors were encountered. Many features of the endocrine physiology in childhood, during puberty, and in adulthood have been characterized. Hormonal evaluations of endocrine disorders of reproduction are characterized on the basis of their characteristic pathophysiologic alterations.", "contents": "Radioligand assay in reproductive biology. Radioligand assays have been developed for the principal reproductive steroids and peptide hormones. Specific binding reagents have included antibodies, plasma binders, and intracellular receptors. In each assay, problems of specificity, sensitivity, and nonspecific inhibitors were encountered. Many features of the endocrine physiology in childhood, during puberty, and in adulthood have been characterized. Hormonal evaluations of endocrine disorders of reproduction are characterized on the basis of their characteristic pathophysiologic alterations."}
{"id": "PMID:1098155", "title": "A clinical evaluation of the efficacy of mebendazole in the treatment of trichuriasis.", "content": "In a single-blind clinical study of mebendazole, a new, broadspectrum anthelmintic, 70 patients with light to severe infections of Trichuris trichiura were treated. Ten subjects received placebos, and 60 received 100 mg mebendazole twice a day for three and four days. The drug was well tolerated by the patients, and no toxic side effects were observed. Egg count comparisons using the McMaster method on feces collected before and one month after treatment indicated a cure rate for trichuriasis of 65.6% and 89.3% and a total egg reduction of 69.5% and 95.9% for three- and four-day regimens, respectively. Patients not cleared showed an  egg reduction rate of 31.6% and 76.8% on the three- and four-day regimens, respectively.", "contents": "A clinical evaluation of the efficacy of mebendazole in the treatment of trichuriasis. In a single-blind clinical study of mebendazole, a new, broadspectrum anthelmintic, 70 patients with light to severe infections of Trichuris trichiura were treated. Ten subjects received placebos, and 60 received 100 mg mebendazole twice a day for three and four days. The drug was well tolerated by the patients, and no toxic side effects were observed. Egg count comparisons using the McMaster method on feces collected before and one month after treatment indicated a cure rate for trichuriasis of 65.6% and 89.3% and a total egg reduction of 69.5% and 95.9% for three- and four-day regimens, respectively. Patients not cleared showed an  egg reduction rate of 31.6% and 76.8% on the three- and four-day regimens, respectively."}
{"id": "PMID:1098157", "title": "Variables in objective audiometry.", "content": "Objective audiometry has a definite application in evaluating problem patients. Three basic tests, electrodermal response audiometry (EDR), evoked cortical response audiometry (ERA), and impedance audiometry, are used to evaluate pediatric and adult patients. Each measurement technic, however, has limitations of which the physician should be aware. Impedance audiometry has the greatest potential as an objective measurement of auditory function.", "contents": "Variables in objective audiometry. Objective audiometry has a definite application in evaluating problem patients. Three basic tests, electrodermal response audiometry (EDR), evoked cortical response audiometry (ERA), and impedance audiometry, are used to evaluate pediatric and adult patients. Each measurement technic, however, has limitations of which the physician should be aware. Impedance audiometry has the greatest potential as an objective measurement of auditory function."}
{"id": "PMID:1098158", "title": "Hereditary nephritis (Alport's syndrome) in a new kindred.", "content": "A new kindred with Alport's syndrome is presented with the clinical and historical data permitting diagnosis. History of family members with nephritis and oto-ophthalmologic anomalies in the presence of nephritis in a given patient continues to be the strongest clue to Alport's syndrome. As the disease is one of the few in which life-threatening renal failure can be thus predicted, increased emphasis on diagnosis is urged in place of casually categorizing these patients together with those who have Bright's disease. Public Law 92-603 is a mandata from the public for physicians to exercise every avenue of diagnosis and salvage for the nephritic patient. Alport's syndrome with nephritis represents an opportunity to plan for eventual dialysis and renal transplantation.", "contents": "Hereditary nephritis (Alport's syndrome) in a new kindred. A new kindred with Alport's syndrome is presented with the clinical and historical data permitting diagnosis. History of family members with nephritis and oto-ophthalmologic anomalies in the presence of nephritis in a given patient continues to be the strongest clue to Alport's syndrome. As the disease is one of the few in which life-threatening renal failure can be thus predicted, increased emphasis on diagnosis is urged in place of casually categorizing these patients together with those who have Bright's disease. Public Law 92-603 is a mandata from the public for physicians to exercise every avenue of diagnosis and salvage for the nephritic patient. Alport's syndrome with nephritis represents an opportunity to plan for eventual dialysis and renal transplantation."}
{"id": "PMID:1098159", "title": "Indications for fiberoptic colonscopy.", "content": "In certain clinical situations, fiberoptic colonoscopy has proved most useful and effective as a diagnostic and therapeutic tool. Colonscopy is indicated to remove polyps when feasible and to rule out the presence of toher polyps or tumors undetected by barium enema. Patients with cancer of the colon should have preoperative colonoscopy to rule out the presence of undetected polyps or metachronous cancer. Follow-up examinations are important to insure against residual cancer or recurrence after colonscopic removal of sessile, premalianant, or malignant polypoid lesions, and to evaluate certain patients who have increased risk of polyp or tumor formation. Colonscopy usually can explain persistent, localized abnormalities detected by barium enema (ie, \"filling defects\") and provide a definitive diagnosis in cases of unexplained rectal bleeding or diarrhea despite negative sigmoidoscopic and barium studies. In experienced hands, this technic is safe, confortable, and effective in avoiding laparotomy to remove clinically significant polyps and in providing definitive diagnosis in many clinical situations.", "contents": "Indications for fiberoptic colonscopy. In certain clinical situations, fiberoptic colonoscopy has proved most useful and effective as a diagnostic and therapeutic tool. Colonscopy is indicated to remove polyps when feasible and to rule out the presence of toher polyps or tumors undetected by barium enema. Patients with cancer of the colon should have preoperative colonoscopy to rule out the presence of undetected polyps or metachronous cancer. Follow-up examinations are important to insure against residual cancer or recurrence after colonscopic removal of sessile, premalianant, or malignant polypoid lesions, and to evaluate certain patients who have increased risk of polyp or tumor formation. Colonscopy usually can explain persistent, localized abnormalities detected by barium enema (ie, \"filling defects\") and provide a definitive diagnosis in cases of unexplained rectal bleeding or diarrhea despite negative sigmoidoscopic and barium studies. In experienced hands, this technic is safe, confortable, and effective in avoiding laparotomy to remove clinically significant polyps and in providing definitive diagnosis in many clinical situations."}
{"id": "PMID:1098169", "title": "Problems related to postmenopausal women.", "content": "The most consistently troublesome symptom of the menopause is the hot flush. It can occur without warning or may be triggered off by emotion, exercise or change of environment or temperature. It is estimated that about 50% of women suffer from hot flushes, but only 5-10% of these seek medical advice. The cause is ascribed to excessive production of follicle-stimulating hormone. There is an increased sensitivity to the effect of locally released dilator substances in the peripheral vasculature.", "contents": "Problems related to postmenopausal women. The most consistently troublesome symptom of the menopause is the hot flush. It can occur without warning or may be triggered off by emotion, exercise or change of environment or temperature. It is estimated that about 50% of women suffer from hot flushes, but only 5-10% of these seek medical advice. The cause is ascribed to excessive production of follicle-stimulating hormone. There is an increased sensitivity to the effect of locally released dilator substances in the peripheral vasculature."}
{"id": "PMID:1098171", "title": "[Death and the moment of death: several legal aspects].", "content": "None of the important legal issues concerning the moment of death and re-animation procedures can be solved by means of simplistic legal formulae. In adjudicating on a particular problem, a court of law will have to be guided by prevailing religious, societal and medical attitudes. But as jurists we must strongly support the individual's \"right to die\".", "contents": "[Death and the moment of death: several legal aspects]. None of the important legal issues concerning the moment of death and re-animation procedures can be solved by means of simplistic legal formulae. In adjudicating on a particular problem, a court of law will have to be guided by prevailing religious, societal and medical attitudes. But as jurists we must strongly support the individual's \"right to die\"."}
{"id": "PMID:1098175", "title": "Osteonecrosis following renal transplantation.", "content": "Subarticular osteonecrosis has been described with increasing frequency after renal transplantation and the use of immunosuppressive corticosteroids. Six cases have been encountered among 170 patients in the Johannesburg renal transplantation programme. The commonly affected sites are the femoral head, the femoral condyles, the humeral head, the talus and the capitulum. The radiological and morbid anatomical features of this complication are described, alternative theories of pathogenesis are considered and an approach to treatment is outlined.", "contents": "Osteonecrosis following renal transplantation. Subarticular osteonecrosis has been described with increasing frequency after renal transplantation and the use of immunosuppressive corticosteroids. Six cases have been encountered among 170 patients in the Johannesburg renal transplantation programme. The commonly affected sites are the femoral head, the femoral condyles, the humeral head, the talus and the capitulum. The radiological and morbid anatomical features of this complication are described, alternative theories of pathogenesis are considered and an approach to treatment is outlined."}
{"id": "PMID:1098177", "title": "A bismuth-peptide complex in the treatment of duodenal ulceration. A double-blind duodenoscopic study.", "content": "A bismuth-protein complex (Bicitropeptide, BCP-Compound) active at an acid pH was studied in a trial of treatment in ambulant patients with duodenal ulcers. BCP is said to have an affinity for actively granulating tissue, and to coat the ulcer with a protective layer, allowing healing to occur. A double-blind trial of BCP and placebo was undertaken in 60 patients with duodenal ulcers. Assessments were made by duodenoscopy. Healing of the ulcers occurred in 89% of patients on BCP, and the mean time for the disappearance of symptoms on BCP was 4,9 days. Only 16% of ulcers healed on placebo, and the mean time for disappearance of symptoms on placebo was 6,8 days. This result is highly significant and in favour of BCP (X-2 equals 30,52 P less than 0,0005). No side-effects were observed.", "contents": "A bismuth-peptide complex in the treatment of duodenal ulceration. A double-blind duodenoscopic study. A bismuth-protein complex (Bicitropeptide, BCP-Compound) active at an acid pH was studied in a trial of treatment in ambulant patients with duodenal ulcers. BCP is said to have an affinity for actively granulating tissue, and to coat the ulcer with a protective layer, allowing healing to occur. A double-blind trial of BCP and placebo was undertaken in 60 patients with duodenal ulcers. Assessments were made by duodenoscopy. Healing of the ulcers occurred in 89% of patients on BCP, and the mean time for the disappearance of symptoms on BCP was 4,9 days. Only 16% of ulcers healed on placebo, and the mean time for disappearance of symptoms on placebo was 6,8 days. This result is highly significant and in favour of BCP (X-2 equals 30,52 P less than 0,0005). No side-effects were observed."}
{"id": "PMID:1098178", "title": "[The rebel ambulance of 1914].", "content": "The story of the so-called Rebel Ambulance of 1914 covers a period of about 6 weeks. During this time a group of people in Bloemfontein endeavoured to provide medical cover and comforts for casualties among those who participated in the armed protest of 1914 in the Orange Free State. Information about relevant historical facts, the collection of funds and equipment, and the work of the field ambulance are presented.", "contents": "[The rebel ambulance of 1914]. The story of the so-called Rebel Ambulance of 1914 covers a period of about 6 weeks. During this time a group of people in Bloemfontein endeavoured to provide medical cover and comforts for casualties among those who participated in the armed protest of 1914 in the Orange Free State. Information about relevant historical facts, the collection of funds and equipment, and the work of the field ambulance are presented."}
{"id": "PMID:1098179", "title": "The effect of vehicle composition on the release of chloramphenicol from creams and eye ointments.", "content": "The effects that formulation of topical vehicle can have on the bio-availability and effectiveness of active ingrediants are discussed with reference to steroids, salicylic acid and benzocaine.  Results obtained for chloramphenicol-containing preparations are presented, and both dissolution curves and cup-plate assays demonstrate that chloramphenicol has far superior release (and hence activity) from creams than from ophthalmic ointments.  The latter, however, produce adequate zones of inhibition, and are long-acting.", "contents": "The effect of vehicle composition on the release of chloramphenicol from creams and eye ointments. The effects that formulation of topical vehicle can have on the bio-availability and effectiveness of active ingrediants are discussed with reference to steroids, salicylic acid and benzocaine.  Results obtained for chloramphenicol-containing preparations are presented, and both dissolution curves and cup-plate assays demonstrate that chloramphenicol has far superior release (and hence activity) from creams than from ophthalmic ointments.  The latter, however, produce adequate zones of inhibition, and are long-acting."}
{"id": "PMID:1098180", "title": "The first fifty - a review of the South African Medical Congresses.", "content": "This historical r\u00e9sum\u00e9, a collation of facts from various sources which lie scattered among the pages of early medical journals of our land, has been composed in an attempt to give some continuity of substance to this particular part of South African medical history which will be celebrated by our Jubilee Congress in Johannesburg this year.", "contents": "The first fifty - a review of the South African Medical Congresses. This historical r\u00e9sum\u00e9, a collation of facts from various sources which lie scattered among the pages of early medical journals of our land, has been composed in an attempt to give some continuity of substance to this particular part of South African medical history which will be celebrated by our Jubilee Congress in Johannesburg this year."}
{"id": "PMID:1098181", "title": "A preliminary report on cephradine.", "content": "Plasma cephradine levels were measured after parenteral and oral administration to healthy adults. Plasma cephradine contrations of 7 - 15 mug/ml were measured after 0.5 g had been administered by mouth, and concentrations of 12 - 57 mug/ml were measured after parenteral administration; they fell to 3 mug/ml within 240 minutes except after intramuscular administration. The results of an open clinical trial conducted among 10 patients suffering from urinary tract infections were satisfactory.", "contents": "A preliminary report on cephradine. Plasma cephradine levels were measured after parenteral and oral administration to healthy adults. Plasma cephradine contrations of 7 - 15 mug/ml were measured after 0.5 g had been administered by mouth, and concentrations of 12 - 57 mug/ml were measured after parenteral administration; they fell to 3 mug/ml within 240 minutes except after intramuscular administration. The results of an open clinical trial conducted among 10 patients suffering from urinary tract infections were satisfactory."}
{"id": "PMID:1098183", "title": "Streptococcal cellulitis of the scrotum and penis with secondary skin gangrene.", "content": "Cellulitis of the scrotum and penis is caused, in the majority of instances, by a beta hemolytic streptococci without a discernible portal of entry. Clostridium, occasionally, will result in this disease as a manifestation of a perirectal abscess. In either instance, fluid accumulates rapidly in the closed space between Colles' and Buck's fascia, producing intense swelling of the scrotum. If this compartment is not immediately decompressed by linear incisions, devascularization of the scrotal and penile skin will often occur, resulting in gangrene. Immediate treatment of the bacterial infection with penicillin also is essential. If gangrene does develop, radical debridement of the necrotic tissue as well as a wide margin of adjacent inflamed skin must be undertaken. Continual monitoring of the microflora of the debrided would is essential for the selection of the appropriate antibiotic against any secondary intruders. Coverage of the granulating would is accomplished when the would bacterial count is below 10-5 per gram of tissue.", "contents": "Streptococcal cellulitis of the scrotum and penis with secondary skin gangrene. Cellulitis of the scrotum and penis is caused, in the majority of instances, by a beta hemolytic streptococci without a discernible portal of entry. Clostridium, occasionally, will result in this disease as a manifestation of a perirectal abscess. In either instance, fluid accumulates rapidly in the closed space between Colles' and Buck's fascia, producing intense swelling of the scrotum. If this compartment is not immediately decompressed by linear incisions, devascularization of the scrotal and penile skin will often occur, resulting in gangrene. Immediate treatment of the bacterial infection with penicillin also is essential. If gangrene does develop, radical debridement of the necrotic tissue as well as a wide margin of adjacent inflamed skin must be undertaken. Continual monitoring of the microflora of the debrided would is essential for the selection of the appropriate antibiotic against any secondary intruders. Coverage of the granulating would is accomplished when the would bacterial count is below 10-5 per gram of tissue."}
{"id": "PMID:1098185", "title": "The bacterial causation of postoperative osteitis pubis.", "content": "Five patients with a bacterial causation of postoperative osteitis pubis were treated during a three year period.  In each patient, the bacterial cause was suggested by a known infectious process adjacent of the symphysis pubis.  It is suggested that a bacterial causation be considered for the clinical and roentgenographic syndrome of osteitis pubis if a contigous infectious process is present.", "contents": "The bacterial causation of postoperative osteitis pubis. Five patients with a bacterial causation of postoperative osteitis pubis were treated during a three year period.  In each patient, the bacterial cause was suggested by a known infectious process adjacent of the symphysis pubis.  It is suggested that a bacterial causation be considered for the clinical and roentgenographic syndrome of osteitis pubis if a contigous infectious process is present."}
{"id": "PMID:1098187", "title": "Fulton and penicillin.", "content": "John Fulton, the late noted neurophysiologist, played a little known role in bringing penicillin to the world. He contributed behind-the-scenes support to Howard Florey and the penicillin research and production.", "contents": "Fulton and penicillin. John Fulton, the late noted neurophysiologist, played a little known role in bringing penicillin to the world. He contributed behind-the-scenes support to Howard Florey and the penicillin research and production."}
{"id": "PMID:1098189", "title": "Failure of angiotensin II inhibitor to modify hyperacute rejection.", "content": "The effect of angiotensin II inhibitor (sar1,gly8 angiotensin II) upon the pig-to-dog (10 pigs to 20 dogs) modified renal xenograft model has been studied. The angiotensin II inhibitor does not prevent hyperacute rejection (HAR). This implies that the vasocaonstriction which occurs during the HAR is not related to high levels of angiotensin II.", "contents": "Failure of angiotensin II inhibitor to modify hyperacute rejection. The effect of angiotensin II inhibitor (sar1,gly8 angiotensin II) upon the pig-to-dog (10 pigs to 20 dogs) modified renal xenograft model has been studied. The angiotensin II inhibitor does not prevent hyperacute rejection (HAR). This implies that the vasocaonstriction which occurs during the HAR is not related to high levels of angiotensin II."}
{"id": "PMID:1098190", "title": "The use of monofilament suture in vascular surgery.", "content": "The blood lost through the suture line holes of vessels closed with braided polyester sutures is compared with that lost when a monofilament polypropylene suture is employed. In a retrospective analysis of blood replacement during saphenous vein aortorenal bypasses, twice as much blood replacement was required when the braided polyester suture was used. Further, ten dogs had one femoral artery reanastomosed with braided polyester suture and the other with monofilament polypropylene. Bleeding was three times greater and lasted six times longer when the braided polyester suture material was utilized.", "contents": "The use of monofilament suture in vascular surgery. The blood lost through the suture line holes of vessels closed with braided polyester sutures is compared with that lost when a monofilament polypropylene suture is employed. In a retrospective analysis of blood replacement during saphenous vein aortorenal bypasses, twice as much blood replacement was required when the braided polyester suture was used. Further, ten dogs had one femoral artery reanastomosed with braided polyester suture and the other with monofilament polypropylene. Bleeding was three times greater and lasted six times longer when the braided polyester suture material was utilized."}
{"id": "PMID:1098191", "title": "Pseudoaneurysm of the brachiocephalic arteries: a complication of percutaneous internal jugular vein catheterization.", "content": "Three cases of arterial pseudoaneurysm formation as a complication of percutaneous internal jugular vein catheterization are presented. Laceration of the posterior common carotid artery or thyrocervical trunk was the source for these lesions. The common antecedent event was the attempted cannulation of the internal jugular vein with the lateral approach. A transcervical rather than transthoracic approach for treatment of these lesions was selected because of its low morbidity rate. This report documents another complication of internal jugular vein cannulation and recommends methods for prevention and successful treatment should it occur.", "contents": "Pseudoaneurysm of the brachiocephalic arteries: a complication of percutaneous internal jugular vein catheterization. Three cases of arterial pseudoaneurysm formation as a complication of percutaneous internal jugular vein catheterization are presented. Laceration of the posterior common carotid artery or thyrocervical trunk was the source for these lesions. The common antecedent event was the attempted cannulation of the internal jugular vein with the lateral approach. A transcervical rather than transthoracic approach for treatment of these lesions was selected because of its low morbidity rate. This report documents another complication of internal jugular vein cannulation and recommends methods for prevention and successful treatment should it occur."}
{"id": "PMID:1098194", "title": "George J. Heuer, M.D.--a critical analysis of the role that he played in introducing the residency system of training.", "content": "This presentation of the career of George J. Heuer concerns one William S. Halsted's most trusted and esteemed trainees. Many of the facts concerning Dr. Heuer are known but there also are many that are not known and the purpose of this discourse is to record his important contributions involving the training of surgeons as we know it today. Dr. Heuer had many obstacles to overcome in establishing residency training programs in Cincinnati and New York City, but he was able to overcome most of them with the able assistance of his dedicated lieutenants.", "contents": "George J. Heuer, M.D.--a critical analysis of the role that he played in introducing the residency system of training. This presentation of the career of George J. Heuer concerns one William S. Halsted's most trusted and esteemed trainees. Many of the facts concerning Dr. Heuer are known but there also are many that are not known and the purpose of this discourse is to record his important contributions involving the training of surgeons as we know it today. Dr. Heuer had many obstacles to overcome in establishing residency training programs in Cincinnati and New York City, but he was able to overcome most of them with the able assistance of his dedicated lieutenants."}
{"id": "PMID:1098195", "title": "Immunodepression after major surgery in normal patients.", "content": "Multiple parameters of in vivo and in vitro immune function were measured serially before, during, and following nephrectomy in 12 normal renal transplant donors. All in vitro functions studied (total blood lymphocyte count, B-cell count, T-cell count, mitogen blastogenic response, and mixed leukocyte reactivity as both responder and stimulator) decreased on induction of anethesia and continued to fall during and after operation to reach a low point on the evening after nephrectomy. Depth of depression and rate of recovery varied with the individual function, but all were near normal by the fifth postoperative day. The in vivo delayed hypersensitivity response to cutaneously administered recall antigens declined more gradually and was still falling at the fifth postoperative day. Return of preoperative skin response was delayed, being complete for streptokinase/streptodornase (SK/SD) by 10 to 14 days but incomplete as long as 2 to 3 weeks for mumps and Candida antigens. Serum immunoglobulins did not change. These findings suggest incomplete correlation among the responses to the commonly used in vitro assays of cellular immunity and poor correlation with the in vivo tests. Although surgery and anesthesia results in measurable depression of immune response, clinically significant problems did not arise in these patients.", "contents": "Immunodepression after major surgery in normal patients. Multiple parameters of in vivo and in vitro immune function were measured serially before, during, and following nephrectomy in 12 normal renal transplant donors. All in vitro functions studied (total blood lymphocyte count, B-cell count, T-cell count, mitogen blastogenic response, and mixed leukocyte reactivity as both responder and stimulator) decreased on induction of anethesia and continued to fall during and after operation to reach a low point on the evening after nephrectomy. Depth of depression and rate of recovery varied with the individual function, but all were near normal by the fifth postoperative day. The in vivo delayed hypersensitivity response to cutaneously administered recall antigens declined more gradually and was still falling at the fifth postoperative day. Return of preoperative skin response was delayed, being complete for streptokinase/streptodornase (SK/SD) by 10 to 14 days but incomplete as long as 2 to 3 weeks for mumps and Candida antigens. Serum immunoglobulins did not change. These findings suggest incomplete correlation among the responses to the commonly used in vitro assays of cellular immunity and poor correlation with the in vivo tests. Although surgery and anesthesia results in measurable depression of immune response, clinically significant problems did not arise in these patients."}
{"id": "PMID:1098214", "title": "National Conference on Thrombosis and Hemostosis, Dallas, Tesas 20.-22. November 1974. Clinical Correlations.", "content": "There are two aspects which are clinically relevant in the relationships among thrombosis and arteriosclerosis. First, the relationship of thrombosis to atherogenesis. Thrombosis is closely related to endothelial injury and there is recent evidence that repeated or continuous endothelial damage leads to the development of all the lesions seen in human atherosclerosis. This occurs in the absence of dietary lipid supplement. The relative importance of the parts played by injury and thrombosis in this process need further delineation. Secondly, established arteriosclerotic disease is associated with thrombosis. This thrombosis is us  ually mural, seldom occlusive. Embolism by platelet aggregates which has been well described in the retinal and cerebral circulations may affect other vascular territories such as the heart and the kidney. Such a mechanism may explain some cases of otherwise unexplained sudden cardiac death and some cases of hypertension. We need better clinical tool to detect the occurrence of thrombo-embolism and to monitor it's progress. Measures to modify thrombosis and embolism may be as useful or more useful than those wer currently employ in the clinical management of atherosclerotic disease.", "contents": "National Conference on Thrombosis and Hemostosis, Dallas, Tesas 20.-22. November 1974. Clinical Correlations. There are two aspects which are clinically relevant in the relationships among thrombosis and arteriosclerosis. First, the relationship of thrombosis to atherogenesis. Thrombosis is closely related to endothelial injury and there is recent evidence that repeated or continuous endothelial damage leads to the development of all the lesions seen in human atherosclerosis. This occurs in the absence of dietary lipid supplement. The relative importance of the parts played by injury and thrombosis in this process need further delineation. Secondly, established arteriosclerotic disease is associated with thrombosis. This thrombosis is us  ually mural, seldom occlusive. Embolism by platelet aggregates which has been well described in the retinal and cerebral circulations may affect other vascular territories such as the heart and the kidney. Such a mechanism may explain some cases of otherwise unexplained sudden cardiac death and some cases of hypertension. We need better clinical tool to detect the occurrence of thrombo-embolism and to monitor it's progress. Measures to modify thrombosis and embolism may be as useful or more useful than those wer currently employ in the clinical management of atherosclerotic disease."}
{"id": "PMID:1098216", "title": "The phase II urokinase-streptokinase pulmonary embolism trial: a national cooperative study.", "content": "The controlled clinical trials of thrombolytic agents in the United States have been carried out in two phases, under the auspices of the National Heart and Lung Institute. Phase I was devoted to the comparison of 12-hour Urokinase (12h-UK) followed by heparin (H) with heparin alone in patients with acute pulmonary embolism (Walsh et al. 1969). The results showed that pateints treated with UK had more rapid and gretaer resolution of pulmonary thromboemboli in the first twenty-four hours of therapy than patients treated with H alone, as assessed by serial pulmonary angiography, hemodynamics and lung scanning (The Urokinase Pulmonary Embolism Trial, 1970, 1973; Hyers et al. 1970). Because of the ralatively small size of the Trial and the low mor tality of treated pulmonary embolism, mortality differences were not sought-nor was one found. Although there was early difference in amount of clot resolution, patients treated with H alone showed similar improvement by two weeks. The phase II Urokinase-Streptokinase Pulmonary Embolism Trial (USPET) was begun to assess the comparative results of UK and Streptokinase (SK) therapy. Because of favorable results obtained with SK in other countries, it was deemed necessary to make this comparison (Browse and James, 1964; Hirsh et al. 1968; Miller et al. 1969, 1971; Chesterman et al. 1969). A third group, 12-hour UK, was added to relate this study (24-hour UK and SK) with the Phase I results which employed only a 12-hour infusion of UK. This Phase II Trial represents the first controlled, randomized study of UK and SK in thromboembolic disorders.", "contents": "The phase II urokinase-streptokinase pulmonary embolism trial: a national cooperative study. The controlled clinical trials of thrombolytic agents in the United States have been carried out in two phases, under the auspices of the National Heart and Lung Institute. Phase I was devoted to the comparison of 12-hour Urokinase (12h-UK) followed by heparin (H) with heparin alone in patients with acute pulmonary embolism (Walsh et al. 1969). The results showed that pateints treated with UK had more rapid and gretaer resolution of pulmonary thromboemboli in the first twenty-four hours of therapy than patients treated with H alone, as assessed by serial pulmonary angiography, hemodynamics and lung scanning (The Urokinase Pulmonary Embolism Trial, 1970, 1973; Hyers et al. 1970). Because of the ralatively small size of the Trial and the low mor tality of treated pulmonary embolism, mortality differences were not sought-nor was one found. Although there was early difference in amount of clot resolution, patients treated with H alone showed similar improvement by two weeks. The phase II Urokinase-Streptokinase Pulmonary Embolism Trial (USPET) was begun to assess the comparative results of UK and Streptokinase (SK) therapy. Because of favorable results obtained with SK in other countries, it was deemed necessary to make this comparison (Browse and James, 1964; Hirsh et al. 1968; Miller et al. 1969, 1971; Chesterman et al. 1969). A third group, 12-hour UK, was added to relate this study (24-hour UK and SK) with the Phase I results which employed only a 12-hour infusion of UK. This Phase II Trial represents the first controlled, randomized study of UK and SK in thromboembolic disorders."}
{"id": "PMID:1098221", "title": "The search for a human equivalent of the mouse T-locus - negative results from a study of HL-A types in spina bifida.", "content": "The spina bifida/anencephaly complex in man in some ways resembles the effects of T-locus mutants in the mouse. To test the hypothesis that these entities are genetically related, HL-A typing was performed in families with multiple cases of central nervous system abnormality. No evidence of linkage was found between HL-A type and the congenital malformation.", "contents": "The search for a human equivalent of the mouse T-locus - negative results from a study of HL-A types in spina bifida. The spina bifida/anencephaly complex in man in some ways resembles the effects of T-locus mutants in the mouse. To test the hypothesis that these entities are genetically related, HL-A typing was performed in families with multiple cases of central nervous system abnormality. No evidence of linkage was found between HL-A type and the congenital malformation."}
{"id": "PMID:1098222", "title": "Renal transplantation and cancer. The Scandia transplant material.", "content": "Between 1965 and 1973, 30 malignant tumours were found in patients transplanted in Scandinavia. Of these tumours, 24 were found in the 1,254 patients transplanted in Scandinavia as part of the Scandia Tranplant Programme between 1969 and 1972. Using the information recorded in the programme, 418 Danish patients who received their first and only transplant between 1969 and 1972 were selected and followed until 31/12 1973. Twelve tumours occurred in these patients. A comparison with the expected age, sex and time-specific incidence rates from the Danish Cancer Register was made and the observed tumour incidence was found to be significantly (P less than 0.001) greater than expected. Tumour incidence was found to be associated with incompatibility in the FOUR series, but was unrelated to number of rejections, the presence of HL-A antibodies and sex difference between donor and recipient. Apart from certain special forms of immunosuppression which could be oncogenic, the cause of tumour development appears to be multifactorial, both the allograft itself and immunosuppressive treatment playing a role.", "contents": "Renal transplantation and cancer. The Scandia transplant material. Between 1965 and 1973, 30 malignant tumours were found in patients transplanted in Scandinavia. Of these tumours, 24 were found in the 1,254 patients transplanted in Scandinavia as part of the Scandia Tranplant Programme between 1969 and 1972. Using the information recorded in the programme, 418 Danish patients who received their first and only transplant between 1969 and 1972 were selected and followed until 31/12 1973. Twelve tumours occurred in these patients. A comparison with the expected age, sex and time-specific incidence rates from the Danish Cancer Register was made and the observed tumour incidence was found to be significantly (P less than 0.001) greater than expected. Tumour incidence was found to be associated with incompatibility in the FOUR series, but was unrelated to number of rejections, the presence of HL-A antibodies and sex difference between donor and recipient. Apart from certain special forms of immunosuppression which could be oncogenic, the cause of tumour development appears to be multifactorial, both the allograft itself and immunosuppressive treatment playing a role."}
{"id": "PMID:1098223", "title": "A new method for the screening of unidentified complaints syndrome in pre-, mid- and post-menopausal women.", "content": "A new simple test for the screening of pre-, mid- and post-menopausal women with unidentified complaints overlaid by psychiatric disorders has been devised. This test is performed by intravenous injection of conjugated estrogen and by comparing Kupperman's menopausal indices before and after the injection. That is, the discriminant function between groups with and without psychiatric abnormalities was obtained from Kupperman's menopausal indices before and after intravenous injection of conjugated estrogen, and from this function was made a calculative differential diagnosis between the two groups. The calculative diagnosis agreed fairly well with the clinical diagnosis by the psychiatrist.", "contents": "A new method for the screening of unidentified complaints syndrome in pre-, mid- and post-menopausal women. A new simple test for the screening of pre-, mid- and post-menopausal women with unidentified complaints overlaid by psychiatric disorders has been devised. This test is performed by intravenous injection of conjugated estrogen and by comparing Kupperman's menopausal indices before and after the injection. That is, the discriminant function between groups with and without psychiatric abnormalities was obtained from Kupperman's menopausal indices before and after intravenous injection of conjugated estrogen, and from this function was made a calculative differential diagnosis between the two groups. The calculative diagnosis agreed fairly well with the clinical diagnosis by the psychiatrist."}
{"id": "PMID:1098228", "title": "[Studies of the method and distribution of prosthetic care].", "content": "Studies on the kind and the distribution of prothetic care. The present paper gives a survey of the achievements of the Prosthetic Service of the Stomatological Clinic at Jena during the years 1961-1970. The studies involved the number of patients who had presented for prosthetic treatment, the most frequent age groups and the kind and extent of prosthetic work.", "contents": "[Studies of the method and distribution of prosthetic care]. Studies on the kind and the distribution of prothetic care. The present paper gives a survey of the achievements of the Prosthetic Service of the Stomatological Clinic at Jena during the years 1961-1970. The studies involved the number of patients who had presented for prosthetic treatment, the most frequent age groups and the kind and extent of prosthetic work."}
{"id": "PMID:1098229", "title": "[Clinical aspects and etiology of prosthesis-induced changes in the oral mucosa].", "content": "The clinical picture and the aetiology of prosthesis-induced alterations of the oral mucosa are presented. In the light of personal gerontological observations, the authors deal with the mechanical irritation produced by the prosthesis as the main aetiological factor and discuss its effects on prophylaxis, diagnosis and therapy.", "contents": "[Clinical aspects and etiology of prosthesis-induced changes in the oral mucosa]. The clinical picture and the aetiology of prosthesis-induced alterations of the oral mucosa are presented. In the light of personal gerontological observations, the authors deal with the mechanical irritation produced by the prosthesis as the main aetiological factor and discuss its effects on prophylaxis, diagnosis and therapy."}
{"id": "PMID:1098231", "title": "[ Problems of wound dressing after gingivectomy in the periodontally diseased incomplete dentition (report of treatment) ].", "content": "After some remarks upon the value and the importance of periodontal surgical dressing in general and in case of incomplete dentition in particular, the author reports of a case in which a temporary plastic bridge had been combined with a dressing plate. This combination may also be recommended as a model to busy stomatological practices.", "contents": "[ Problems of wound dressing after gingivectomy in the periodontally diseased incomplete dentition (report of treatment) ]. After some remarks upon the value and the importance of periodontal surgical dressing in general and in case of incomplete dentition in particular, the author reports of a case in which a temporary plastic bridge had been combined with a dressing plate. This combination may also be recommended as a model to busy stomatological practices."}
{"id": "PMID:1098234", "title": "A reconsideration of immunological privilege within the anterior chamber of the eye.", "content": "The immunologically privileged status of the anterior chamber of the eye has been confirmed on the basis of skin and thyroid allografts with use of inbred strains of rats. Skin grafts, transplanted across both major and minor histocompatibility barriers, enjoy prolonged survival within the anterior chamber. However, several factors have been found to restrict the privilege exhibited by this site: the magnitude of the immunogenetic disparity between donor and recipient, graft size, type of tissue grafted, and, at least in the case of thyroid grafts, the endocrine status of the host. Although a defect in the afferent limb of the immune response has been the popular explanation offered for immunological privilege in an alymphatic site, this was not the basis of the privilege encountered in the anterior chamber. Furthermore, because the efferent limb of the immunological reflex are within the anterior chamber has been demonstrated to be intact, it was suggested that the unique properties of the anterior chamber may depend upon the forced intravascular presentation of antigen and consequent aberrant central processing.", "contents": "A reconsideration of immunological privilege within the anterior chamber of the eye. The immunologically privileged status of the anterior chamber of the eye has been confirmed on the basis of skin and thyroid allografts with use of inbred strains of rats. Skin grafts, transplanted across both major and minor histocompatibility barriers, enjoy prolonged survival within the anterior chamber. However, several factors have been found to restrict the privilege exhibited by this site: the magnitude of the immunogenetic disparity between donor and recipient, graft size, type of tissue grafted, and, at least in the case of thyroid grafts, the endocrine status of the host. Although a defect in the afferent limb of the immune response has been the popular explanation offered for immunological privilege in an alymphatic site, this was not the basis of the privilege encountered in the anterior chamber. Furthermore, because the efferent limb of the immunological reflex are within the anterior chamber has been demonstrated to be intact, it was suggested that the unique properties of the anterior chamber may depend upon the forced intravascular presentation of antigen and consequent aberrant central processing."}
{"id": "PMID:1098235", "title": "The effect of cyproheptadine (periactin) on rejection of rat kidney allografts.", "content": "The effect of cyproheptadine, a potent platelet deaggregator, was investigated in rats bearing renal allografts. Thirty-three bilaterally nephrectomized LEW rats received orthotopic transplants of a (LEW X BN)F-1 kidney. Fourteen rats were treated with cyproheptadine, 1.5 mg/kg i.m. daily, for 60 days after transplantation; the remaining animals served as controls. The median allograft survival time in the cyproheptadine group was prolonged to 64 days compared to 12 days in the untreated group (P less than 0.005). In the cyproheptadine group the blood urea nitrogen rose to over 150 mg/100 ml 1 week after transplantation, fell to 80-120 mg/100 ml, and then rose steadily from the day after transplantation until the death of the animals. Despite prolonged survival, cyproheptadine-treated animals usually showed the histological findings of acute and chronic renal rejection. Cyproheptadine may be a useful adjunct to immunosuppression in the management of human renal allotransplantation.", "contents": "The effect of cyproheptadine (periactin) on rejection of rat kidney allografts. The effect of cyproheptadine, a potent platelet deaggregator, was investigated in rats bearing renal allografts. Thirty-three bilaterally nephrectomized LEW rats received orthotopic transplants of a (LEW X BN)F-1 kidney. Fourteen rats were treated with cyproheptadine, 1.5 mg/kg i.m. daily, for 60 days after transplantation; the remaining animals served as controls. The median allograft survival time in the cyproheptadine group was prolonged to 64 days compared to 12 days in the untreated group (P less than 0.005). In the cyproheptadine group the blood urea nitrogen rose to over 150 mg/100 ml 1 week after transplantation, fell to 80-120 mg/100 ml, and then rose steadily from the day after transplantation until the death of the animals. Despite prolonged survival, cyproheptadine-treated animals usually showed the histological findings of acute and chronic renal rejection. Cyproheptadine may be a useful adjunct to immunosuppression in the management of human renal allotransplantation."}
{"id": "PMID:1098232", "title": "Therapy against brain swelling in stroke patients. A retrospective clinical study on 227 patients.", "content": "The effectiveness of cerebral antiedema agents in stroke has been questioned. Animal and clinical work is inconclusive about steroids and osmotic drugs. A retrospective study of a continuous series of 227 stroke patients treated in the acute stage (some with dexamethasone alone, some with dexamethasone plus hyperosmotic mannitol infusions, and some without antiedema therapy) showed no significant difference in the ten-day survival rate. On this criterion, there is no ground for the systematic use of such agents against this type of brain swelling.", "contents": "Therapy against brain swelling in stroke patients. A retrospective clinical study on 227 patients. The effectiveness of cerebral antiedema agents in stroke has been questioned. Animal and clinical work is inconclusive about steroids and osmotic drugs. A retrospective study of a continuous series of 227 stroke patients treated in the acute stage (some with dexamethasone alone, some with dexamethasone plus hyperosmotic mannitol infusions, and some without antiedema therapy) showed no significant difference in the ten-day survival rate. On this criterion, there is no ground for the systematic use of such agents against this type of brain swelling."}
{"id": "PMID:1098236", "title": "Shifts from IgG-2 class to IgG-1 class in CBA and C3H anti-BALB/c antibody.", "content": "With use of a recently developed method for determining relative levels of IgG-1 and IgG-2 class antibodies of a given specificity within an unfractionated serum, it has been possible to examine anti-BALB/c antibodies in the early and late part of an immunization with allogeneic spleen cells. At about 6 days after primary immunization of CBA or C3H mice with BALB/c spleen cells, suppressive antibodies can be measured in the sera of the animals. About half of these are attributable to IgM class, and this contribution decreases to zero by the 12th day. The remaining suppressive antibodies are of IgG-2 class and these increase in concentration until day 8 or 12, or begin to decline between day 8 and day 12. Anti-BALB/c antibodies of IgG-1 class have not yet appeared on day 6, but thereafter appear and increase in concentration. Thus, antibodies of IgG-1 class begin to appear after those of the IgG-2 class and may still be increasing after the IgG-2 class has stopped to increase in concentration, antibody of IgG-1 class is continuing to increase and may even continue to increase after IgF-2 class antibody has begun to decrease in concentration. Thus, the synthesis of IgG-1 class antibody begins later and continues later than that of IgG-2 class. The implications of this sequence for our data on various effects of anti-H-2 antibodies on retention of skin allografts are discussed.", "contents": "Shifts from IgG-2 class to IgG-1 class in CBA and C3H anti-BALB/c antibody. With use of a recently developed method for determining relative levels of IgG-1 and IgG-2 class antibodies of a given specificity within an unfractionated serum, it has been possible to examine anti-BALB/c antibodies in the early and late part of an immunization with allogeneic spleen cells. At about 6 days after primary immunization of CBA or C3H mice with BALB/c spleen cells, suppressive antibodies can be measured in the sera of the animals. About half of these are attributable to IgM class, and this contribution decreases to zero by the 12th day. The remaining suppressive antibodies are of IgG-2 class and these increase in concentration until day 8 or 12, or begin to decline between day 8 and day 12. Anti-BALB/c antibodies of IgG-1 class have not yet appeared on day 6, but thereafter appear and increase in concentration. Thus, antibodies of IgG-1 class begin to appear after those of the IgG-2 class and may still be increasing after the IgG-2 class has stopped to increase in concentration, antibody of IgG-1 class is continuing to increase and may even continue to increase after IgF-2 class antibody has begun to decrease in concentration. Thus, the synthesis of IgG-1 class antibody begins later and continues later than that of IgG-2 class. The implications of this sequence for our data on various effects of anti-H-2 antibodies on retention of skin allografts are discussed."}
{"id": "PMID:1098237", "title": "A mixed antiglobulin test with kidney cells in suspension for IgG antibody in human allograft recipients.", "content": "A modification of the mixed antiglobulin test for the detection of IgG antibodies directed against human kidney cells has been devised which uses a suspension of individual kidney cells rather than a monolayer culture as antigen so that it may be enployed clinically as a prospective crossmatch for organ transplantation. The antiglobulin reagent is added to the sensitized kidney cells rather than to the indicator erythrocytes, which reduces the background of nonspecific reations almost to zero. It is reproducible. The mixed antiglobulin test detected antibody in the sera of patients on chronic dialysis two to four times more frequently than did either the immune adherence test or the most sensitive modification of the microlymphocytotoxicity test which was utilized. It detected the development of antibody specific for donor kidney cells in the sera of 5 of 10 allograft receipients during periods of good to moderate renal transplant function several months before rejection.", "contents": "A mixed antiglobulin test with kidney cells in suspension for IgG antibody in human allograft recipients. A modification of the mixed antiglobulin test for the detection of IgG antibodies directed against human kidney cells has been devised which uses a suspension of individual kidney cells rather than a monolayer culture as antigen so that it may be enployed clinically as a prospective crossmatch for organ transplantation. The antiglobulin reagent is added to the sensitized kidney cells rather than to the indicator erythrocytes, which reduces the background of nonspecific reations almost to zero. It is reproducible. The mixed antiglobulin test detected antibody in the sera of patients on chronic dialysis two to four times more frequently than did either the immune adherence test or the most sensitive modification of the microlymphocytotoxicity test which was utilized. It detected the development of antibody specific for donor kidney cells in the sera of 5 of 10 allograft receipients during periods of good to moderate renal transplant function several months before rejection."}
{"id": "PMID:1098233", "title": "Comparison of blood flow and patency in arterial and vein grafts to basilar artery.", "content": "Blood flow and patency rates obtained by lingual to basilar artery anastomosis were compared with those obtained by saphenous vein bypass graft from the carotid to the basilar artey in two groups of ten dogs. Flow was measured by an electromagnetic technique while blood pressure and blood gases were monitored. Graft patency also was determined by angiography and histological examination. The arterial and venous grafts carried more than enough blood to maintain a normal flow (9.5 ml per minute) through the basilar system of dogs. Immediately after anastomosis, average flow through the vein grafts was 15.5 ml per minute (range 10 to 24 ml per minute). Six weeks later, average flow through the vein graft was 11.5 ml per minute and through the arterial graft 13 ml per minute. With induced hypertension, flow increased in the arterial grafts to an average of 26.2 ml per minute and in the vein grafts 24 ml per minute. Hypercarbia increased arterial graft flow to an average of 27.8 ml per minute and vein graft flow to 23.5 ml per minute. By angiography, graft patency was shown in only 80% of the grafts at one week and in 60% at six weeks postoperatively, even though all grafts were patent by flow and histological determinations. This failure of angiography represents a limitation of the radiographical resolution in millimeter-sized vessels.", "contents": "Comparison of blood flow and patency in arterial and vein grafts to basilar artery. Blood flow and patency rates obtained by lingual to basilar artery anastomosis were compared with those obtained by saphenous vein bypass graft from the carotid to the basilar artey in two groups of ten dogs. Flow was measured by an electromagnetic technique while blood pressure and blood gases were monitored. Graft patency also was determined by angiography and histological examination. The arterial and venous grafts carried more than enough blood to maintain a normal flow (9.5 ml per minute) through the basilar system of dogs. Immediately after anastomosis, average flow through the vein grafts was 15.5 ml per minute (range 10 to 24 ml per minute). Six weeks later, average flow through the vein graft was 11.5 ml per minute and through the arterial graft 13 ml per minute. With induced hypertension, flow increased in the arterial grafts to an average of 26.2 ml per minute and in the vein grafts 24 ml per minute. Hypercarbia increased arterial graft flow to an average of 27.8 ml per minute and vein graft flow to 23.5 ml per minute. By angiography, graft patency was shown in only 80% of the grafts at one week and in 60% at six weeks postoperatively, even though all grafts were patent by flow and histological determinations. This failure of angiography represents a limitation of the radiographical resolution in millimeter-sized vessels."}
{"id": "PMID:1098241", "title": "Thymectomy as immunosuppression in uremic patients.", "content": "Five uremic patients awaiting renal transplantation underwent transcervical thymectomy in an evaluation of the immunosuppressive effect of removal of the thymus in such transplantations. A number of immunological parameters, including lymphocyte transformation tests, were followed in these patients for up to 30 weeks after thymectomy. The number of B lymphocytes in the blood, stem cells in bone marrow, and T lymphocytes in lymph nodes decreased, whereas IgG, IgA, and IgM in four cases and C3 complement in all five cases increased. Blood leucocyte and lymphocyte counts did not show any characteristic changes. The T cell response of circulating lymphocytes was determined after stimulation with mitogens, specific antigens, and allogeneic cells in mixed lymphocyte culture, and showed a large increase. Thymectomy of uremic patients results in a considerable increase in immunocompetence in the first 30 weeks, indicating that it is not suitable as an immunosuppressive treatment.", "contents": "Thymectomy as immunosuppression in uremic patients. Five uremic patients awaiting renal transplantation underwent transcervical thymectomy in an evaluation of the immunosuppressive effect of removal of the thymus in such transplantations. A number of immunological parameters, including lymphocyte transformation tests, were followed in these patients for up to 30 weeks after thymectomy. The number of B lymphocytes in the blood, stem cells in bone marrow, and T lymphocytes in lymph nodes decreased, whereas IgG, IgA, and IgM in four cases and C3 complement in all five cases increased. Blood leucocyte and lymphocyte counts did not show any characteristic changes. The T cell response of circulating lymphocytes was determined after stimulation with mitogens, specific antigens, and allogeneic cells in mixed lymphocyte culture, and showed a large increase. Thymectomy of uremic patients results in a considerable increase in immunocompetence in the first 30 weeks, indicating that it is not suitable as an immunosuppressive treatment."}
{"id": "PMID:1098242", "title": "Studies on the stability of antilymphocyte globulins.", "content": "An account is given of the effect of storage under various conditions on the physical and immunosuppressive properties of antilymphocyte globulins. No loss of in vivo immunosuppressive potency was shown by horse antimouse lymphocyte globulin when stored for up to 2 years in a liquid, frozen, or lyophilised state. Similar studies on horse antihuman lymphocyte globulin showed no loss of in vitro activity, as measured by an immunofluorescence test. Ultracentrifugal analyses showed no increase in aggregate when antilymphocyte globulin was stored liquid or freeze-dried at 4 degrees C. Storage experiments at abnormally high temperatures demonstrated that under these conditions freeze-dried horse antihuman lymphocyte globulin was stabilised by the presence of glucose (4.6%w/v). Antilymphocyte globulin lyophilised in the presence of glucose showed improved solubility properties as compared with material dried from saline.", "contents": "Studies on the stability of antilymphocyte globulins. An account is given of the effect of storage under various conditions on the physical and immunosuppressive properties of antilymphocyte globulins. No loss of in vivo immunosuppressive potency was shown by horse antimouse lymphocyte globulin when stored for up to 2 years in a liquid, frozen, or lyophilised state. Similar studies on horse antihuman lymphocyte globulin showed no loss of in vitro activity, as measured by an immunofluorescence test. Ultracentrifugal analyses showed no increase in aggregate when antilymphocyte globulin was stored liquid or freeze-dried at 4 degrees C. Storage experiments at abnormally high temperatures demonstrated that under these conditions freeze-dried horse antihuman lymphocyte globulin was stabilised by the presence of glucose (4.6%w/v). Antilymphocyte globulin lyophilised in the presence of glucose showed improved solubility properties as compared with material dried from saline."}
{"id": "PMID:1098243", "title": "The response of the lymphoid system to renal allografts in sheep.", "content": "The immunological events which occur in lymph nodes situation regional to renal allografts have been studied by collecting lymph from these nodes and monitoring the changes in its cellular and humoral antibody content. Simultaneously, reactions occurring in the renal allograft and in lymph nodes distant to the graft were also monitored in the lymph from these organs throughout thelife of the graft. Large basophilic lymphoid cells appeared first in lymph from the renal allograft at around 48 hr postgrafting, whereas these cells did not appear in the lymph from the regional node until 80-100 hr after the graft was installed. Lymphoid blast cells were not seen inany significant numbers in lumph from nodes situated at a distance from the graft. The first detectable antibody was produced by the regional lymph node between 110 and 175 hr postgrafting, andin other undetermined sites within the next 80 hr. Antibody was not synthesized in any detectable amounts by the graft or by lymph nodes situated at a distance from the graft. The antibody which was present in the lymph from the regional node was produced primarily by fixed cells which remained in the node, and very little antibody was produced by the lymphoid cells which migrated from the node in efferent lymph. The cells in the lymph from the renal allograft produced only small amounts of immunoglobulin. Cells present in lymph from the renal allograft and in lymph from the regional lymph node actively synthesized and secreted significant amounts of nonimmunoglobulin proteins which were separated on Sephadex G-200 columns into peaks which coincided with 19S, 7S, and 4S proteins. The identities and biological activities of these proteins have not yet been determined.", "contents": "The response of the lymphoid system to renal allografts in sheep. The immunological events which occur in lymph nodes situation regional to renal allografts have been studied by collecting lymph from these nodes and monitoring the changes in its cellular and humoral antibody content. Simultaneously, reactions occurring in the renal allograft and in lymph nodes distant to the graft were also monitored in the lymph from these organs throughout thelife of the graft. Large basophilic lymphoid cells appeared first in lymph from the renal allograft at around 48 hr postgrafting, whereas these cells did not appear in the lymph from the regional node until 80-100 hr after the graft was installed. Lymphoid blast cells were not seen inany significant numbers in lumph from nodes situated at a distance from the graft. The first detectable antibody was produced by the regional lymph node between 110 and 175 hr postgrafting, andin other undetermined sites within the next 80 hr. Antibody was not synthesized in any detectable amounts by the graft or by lymph nodes situated at a distance from the graft. The antibody which was present in the lymph from the regional node was produced primarily by fixed cells which remained in the node, and very little antibody was produced by the lymphoid cells which migrated from the node in efferent lymph. The cells in the lymph from the renal allograft produced only small amounts of immunoglobulin. Cells present in lymph from the renal allograft and in lymph from the regional lymph node actively synthesized and secreted significant amounts of nonimmunoglobulin proteins which were separated on Sephadex G-200 columns into peaks which coincided with 19S, 7S, and 4S proteins. The identities and biological activities of these proteins have not yet been determined."}
{"id": "PMID:1098244", "title": "Studies of the behaviour of allogeneic cancellous bone grafts in inbred rats.", "content": "The degree of new bone formation in isografts and allografts has been assessed by a radiotracer technique. Allografts used have been either H-1-identical or H-1-disparate with the recipient. It was found that new bone formation is curtailed within the first 3 weeks after grafting. This curtailment was significantly greater in those donor-recipient combinations which were H-1-identical but disparate for many weak locus antigens than those in which H-1 disparity existed with only a few minor locus differences. There was no late phase (i.e., host-derived) osteogenesis in the combination with H-1 identity, but multiple minor antigen disparities. In isografts and H-1-disparate grafts significant late phase osteogenesis was found in about 30% of recipients. Resorption of the graft was found to be significantly impaired in both H-1-identical and disparate grafts in comparison with isografts. There was a significant negative correlation between weight of the graft and degree of osteogenesis in isografts during the first 2 weeks after grafting, and also during the later phase (6-8 weeks). A similar negative correlation was found during the late phase in H-1-disparate grafts with few minor antigen disparities, but no correlation was found at any time in H-1-identical grafts with multiple minor antigen disparities.", "contents": "Studies of the behaviour of allogeneic cancellous bone grafts in inbred rats. The degree of new bone formation in isografts and allografts has been assessed by a radiotracer technique. Allografts used have been either H-1-identical or H-1-disparate with the recipient. It was found that new bone formation is curtailed within the first 3 weeks after grafting. This curtailment was significantly greater in those donor-recipient combinations which were H-1-identical but disparate for many weak locus antigens than those in which H-1 disparity existed with only a few minor locus differences. There was no late phase (i.e., host-derived) osteogenesis in the combination with H-1 identity, but multiple minor antigen disparities. In isografts and H-1-disparate grafts significant late phase osteogenesis was found in about 30% of recipients. Resorption of the graft was found to be significantly impaired in both H-1-identical and disparate grafts in comparison with isografts. There was a significant negative correlation between weight of the graft and degree of osteogenesis in isografts during the first 2 weeks after grafting, and also during the later phase (6-8 weeks). A similar negative correlation was found during the late phase in H-1-disparate grafts with few minor antigen disparities, but no correlation was found at any time in H-1-identical grafts with multiple minor antigen disparities."}
{"id": "PMID:1098246", "title": "[Study of invertebrate soft tissues by the smear technic in light and electron microscopy].", "content": "A soft tissue is smeared on the surface of an araldite plate, and after fixation is coated by an araldite film. After examination with the light microscope, the araldite slide may be distributed for histological or ultrathin serial processing. The method provides an adequate conservation of cellular structures.", "contents": "[Study of invertebrate soft tissues by the smear technic in light and electron microscopy]. A soft tissue is smeared on the surface of an araldite plate, and after fixation is coated by an araldite film. After examination with the light microscope, the araldite slide may be distributed for histological or ultrathin serial processing. The method provides an adequate conservation of cellular structures."}
{"id": "PMID:1098252", "title": "The pathophysiology of renovascular hypertension.", "content": "Renovascular hypertension, comprising a small percentage of the total hypertensive population, stands out as remarkably important, being the most common cause of surgically correctable hypertension. Its precise diagnosis can be accomplished by judicious application of recently available sophisticated laboratory methods and correct prediction of curability is now possible. Careful selection of cases among the vast numbers of hypertensives by routine screening procedures is essential. The selected few deserve more complete studies including bilateral renal vein renin measurements. Rapid sequence pyelography, split function studies, radioisotope renorgram, and renal arteriography accurately define the presence of a significant structural abnormality involving the renal circulation on either side. Significant bilateral renal vein plasma renin differential, done under appropriate conditions of volume depletion and upright posture, in the absence of interference by concurrent antihypertensive drug therapy, establishes a causal relationship between the structural abnormality and the high blood pressure. A thorough knowledge of the normal physiology of the renin-angiotensin-aldosterone system and its inappropriate response in renovascular hypertension and other related clinical conditions is clearly necessary if one is to plan diagnostic studies intelligently and interpret the results correctly.", "contents": "The pathophysiology of renovascular hypertension. Renovascular hypertension, comprising a small percentage of the total hypertensive population, stands out as remarkably important, being the most common cause of surgically correctable hypertension. Its precise diagnosis can be accomplished by judicious application of recently available sophisticated laboratory methods and correct prediction of curability is now possible. Careful selection of cases among the vast numbers of hypertensives by routine screening procedures is essential. The selected few deserve more complete studies including bilateral renal vein renin measurements. Rapid sequence pyelography, split function studies, radioisotope renorgram, and renal arteriography accurately define the presence of a significant structural abnormality involving the renal circulation on either side. Significant bilateral renal vein plasma renin differential, done under appropriate conditions of volume depletion and upright posture, in the absence of interference by concurrent antihypertensive drug therapy, establishes a causal relationship between the structural abnormality and the high blood pressure. A thorough knowledge of the normal physiology of the renin-angiotensin-aldosterone system and its inappropriate response in renovascular hypertension and other related clinical conditions is clearly necessary if one is to plan diagnostic studies intelligently and interpret the results correctly."}
{"id": "PMID:1098253", "title": "New concepts of the renin system and of vasoconstriction-volume mechanisms. Diagnosis and treatment of renovascular and renal hypertensions.", "content": "Information defining the renin-angiotensin-aldosterone axis as a control system concurrently regulating salt balance and blood pressure has been applied to examine the role of renin in the causation of experimental and clinical forms of renovascular and renal hypertension and thence to develop criteria for differentiating these entities. Experimantally there are two models of renovascular hypertension, one characterized by excess renin with reduced sodium (vasoconstrictor form) and the other by excess sodium with reduced renin (volume form). But with sodium depletion, the volume form switches to a vasoconstrictor form, illustrating how the two factors coordinate to maintain blood pressure. Human renovascular and renal hypertensions appear to be sustained by the same two mechanisms. Experimental and clinical studies both indicate that curable renal hypertension is in fact a renin-dependent vasoconstrictor hypertension. Three criteria, derived from four renin measurements, identify this situation: (1) Hypersecretion of renin is reflected by a high peripheral level when referenced against sodium excretion. (2) Lacteralization of renin secretion with contralateral suppression rules out occult bilateral disease. It is indicated by V-A = 0 from the uninvolved kidney. (3) A third criterion, (V-A)/A greater than 48 per cent from the ipsilat-eral kidney, identifies renal ischemia. These three criteria, when taken together in a combined scoring analysis, provide high precision in identifying the patient with the vasoconstrictor form of renal hypertension that is potentially reversible by appropriate surgery. Absence of these criteria identifies hypertension associated with either occult or overt bilateral renal disease. In these patients, the volume factor often predominates and is expressed by some suppression of plasma renin levels. Here, removal of renal tissue is contraindicated. Corroborative evidence to support these three criteria can be developed from the blood pressure response to angiotensin blocking drugs or to anti-renin therapy with propranolol. Thus in all of these renal hypertensions, the vasoconstrictor and volume factors can be identified using renin measurements and pharmacologic interventions.", "contents": "New concepts of the renin system and of vasoconstriction-volume mechanisms. Diagnosis and treatment of renovascular and renal hypertensions. Information defining the renin-angiotensin-aldosterone axis as a control system concurrently regulating salt balance and blood pressure has been applied to examine the role of renin in the causation of experimental and clinical forms of renovascular and renal hypertension and thence to develop criteria for differentiating these entities. Experimantally there are two models of renovascular hypertension, one characterized by excess renin with reduced sodium (vasoconstrictor form) and the other by excess sodium with reduced renin (volume form). But with sodium depletion, the volume form switches to a vasoconstrictor form, illustrating how the two factors coordinate to maintain blood pressure. Human renovascular and renal hypertensions appear to be sustained by the same two mechanisms. Experimental and clinical studies both indicate that curable renal hypertension is in fact a renin-dependent vasoconstrictor hypertension. Three criteria, derived from four renin measurements, identify this situation: (1) Hypersecretion of renin is reflected by a high peripheral level when referenced against sodium excretion. (2) Lacteralization of renin secretion with contralateral suppression rules out occult bilateral disease. It is indicated by V-A = 0 from the uninvolved kidney. (3) A third criterion, (V-A)/A greater than 48 per cent from the ipsilat-eral kidney, identifies renal ischemia. These three criteria, when taken together in a combined scoring analysis, provide high precision in identifying the patient with the vasoconstrictor form of renal hypertension that is potentially reversible by appropriate surgery. Absence of these criteria identifies hypertension associated with either occult or overt bilateral renal disease. In these patients, the volume factor often predominates and is expressed by some suppression of plasma renin levels. Here, removal of renal tissue is contraindicated. Corroborative evidence to support these three criteria can be developed from the blood pressure response to angiotensin blocking drugs or to anti-renin therapy with propranolol. Thus in all of these renal hypertensions, the vasoconstrictor and volume factors can be identified using renin measurements and pharmacologic interventions."}
{"id": "PMID:1098256", "title": "Renal autotransplantation and ex vivo renal surgery: surgical treatment of renovascular hypertension.", "content": "Autotransplantation of the kidney has proved to be an extremely valuable surgical alternative for the treatment of renovascular hypertension. The modern operation is founded in landmark surgical experiments performed during the early years of this century. Despite the relative technical simplicity and considerable potential application of renal autotransplantation, the operation was not employed for at least a decade following the more uncertain initial clinical adventures in renal allotransplantation. Since its inception, however, the successful use of renal autotransplantation for renovascular hypertension has been well documented in a relatively small but impressive series of clinical situations. Renal autotransplantation in the surgical management of renovascular hypertension appears to afford many technical advantages vis-\u00e0-vis in situ renal revascularization including the option to perform meticulous ex vivo reconstructive surgery. The need to interrupt the renal circulation during renal autotransplantation and the possibility that the ischemic operative interval might result in renal cellular damage, has been a deterrent to greater consideration of this surgical alternative. Cold perfusion of the ischemic kidney has been regarded as \"inherently\" necessary in order to prevent intravascular coagulation and to protect the kidney through the use of hypothermia until the circulation is re-established. A wide variety of perfusates and methods of perfusion have been successfully employed for these purposes. A new perfusate has been formulated which is intended to help prevent the rapid and profound changes in the amounts of distribution of intracellular electrolytes and water which are known to occur when the kidney is deprived of its circulation. This solution appears to be effective in preventing significant damage in ischemic canine kidneys during at least three hours of normothermic exposure, and at least five hours of ambiothermic exposure following a single initial cold perfusion. The various perfusates and methods of renal perfusion for clinical and experimental renal autotransplantation and ex vivo renal surgery are herein compared and contrasted with regard to their proposed mechanisms of action, apparent effectiveness, and simplicity of application.", "contents": "Renal autotransplantation and ex vivo renal surgery: surgical treatment of renovascular hypertension. Autotransplantation of the kidney has proved to be an extremely valuable surgical alternative for the treatment of renovascular hypertension. The modern operation is founded in landmark surgical experiments performed during the early years of this century. Despite the relative technical simplicity and considerable potential application of renal autotransplantation, the operation was not employed for at least a decade following the more uncertain initial clinical adventures in renal allotransplantation. Since its inception, however, the successful use of renal autotransplantation for renovascular hypertension has been well documented in a relatively small but impressive series of clinical situations. Renal autotransplantation in the surgical management of renovascular hypertension appears to afford many technical advantages vis-\u00e0-vis in situ renal revascularization including the option to perform meticulous ex vivo reconstructive surgery. The need to interrupt the renal circulation during renal autotransplantation and the possibility that the ischemic operative interval might result in renal cellular damage, has been a deterrent to greater consideration of this surgical alternative. Cold perfusion of the ischemic kidney has been regarded as \"inherently\" necessary in order to prevent intravascular coagulation and to protect the kidney through the use of hypothermia until the circulation is re-established. A wide variety of perfusates and methods of perfusion have been successfully employed for these purposes. A new perfusate has been formulated which is intended to help prevent the rapid and profound changes in the amounts of distribution of intracellular electrolytes and water which are known to occur when the kidney is deprived of its circulation. This solution appears to be effective in preventing significant damage in ischemic canine kidneys during at least three hours of normothermic exposure, and at least five hours of ambiothermic exposure following a single initial cold perfusion. The various perfusates and methods of renal perfusion for clinical and experimental renal autotransplantation and ex vivo renal surgery are herein compared and contrasted with regard to their proposed mechanisms of action, apparent effectiveness, and simplicity of application."}
{"id": "PMID:1098257", "title": "[Indication, techniques and results of the operative correction of hypospadias using modification of the original Denis Browne technique (author's transl)].", "content": "The experiences with operative correction of hypospadias 416 patients are reported. In 264 of these patients the correction of the hypospadic deformity was completed, using the original Denis Browne technique or a modification described by Marberger. The results and the complications are demonstrated.", "contents": "[Indication, techniques and results of the operative correction of hypospadias using modification of the original Denis Browne technique (author's transl)]. The experiences with operative correction of hypospadias 416 patients are reported. In 264 of these patients the correction of the hypospadic deformity was completed, using the original Denis Browne technique or a modification described by Marberger. The results and the complications are demonstrated."}
{"id": "PMID:1098258", "title": "[First experiences with the incontinence operation Kaufman III (implantation of a silicongel prothesis covered with polyurethanfoam) (author's transl)].", "content": "For operative treatment of urinary incontinence after TUR of the prostate, radical prostatectomy and meningocele operation the silicongel prothesis described by Kaufman was implanted. By compression of the bulbus urethrae a sealing effect was achieved. The pressure of the detrusor opens the urethra enough to enable urination. Seven patients were operated. The results directly after the operations were: three very good, two good and two bad. We achieved very good results by implantation of the prothesis \"large\" -in the case of the two bad operative results we used the \"medium\" size prothesis. By filling these protheses with 5 ml liquid they proved to be leaky. One of these was operatively removed and replaced by the size \"large\". This patient later developed a urethra stricture in the implantation region with overflow bladder after having been continent. By internal urethrotomy this condition was restored; except for minor dripping after miction the patient was continent. The prothesis of a direct postoperative continent patient had to be filled. This led to and infection with the development of fistula. The prothesis had to be removed. A further patient who sat on a hard object developed a lesion of the prothesis with resulting incontinence. At least six months after the operations four patients remained continent and three incontinent.", "contents": "[First experiences with the incontinence operation Kaufman III (implantation of a silicongel prothesis covered with polyurethanfoam) (author's transl)]. For operative treatment of urinary incontinence after TUR of the prostate, radical prostatectomy and meningocele operation the silicongel prothesis described by Kaufman was implanted. By compression of the bulbus urethrae a sealing effect was achieved. The pressure of the detrusor opens the urethra enough to enable urination. Seven patients were operated. The results directly after the operations were: three very good, two good and two bad. We achieved very good results by implantation of the prothesis \"large\" -in the case of the two bad operative results we used the \"medium\" size prothesis. By filling these protheses with 5 ml liquid they proved to be leaky. One of these was operatively removed and replaced by the size \"large\". This patient later developed a urethra stricture in the implantation region with overflow bladder after having been continent. By internal urethrotomy this condition was restored; except for minor dripping after miction the patient was continent. The prothesis of a direct postoperative continent patient had to be filled. This led to and infection with the development of fistula. The prothesis had to be removed. A further patient who sat on a hard object developed a lesion of the prothesis with resulting incontinence. At least six months after the operations four patients remained continent and three incontinent."}
{"id": "PMID:1098259", "title": "[Operative theatment of male pseudohermaphroditism (author's transl)].", "content": "The operative treatment of two patients with male pseudohermaphroditism is reported. The patients reported were girls whose psychosexual attitude indicated a male sexual change. During several procedures the vagina was removed, the urethra was extended according to the method of Denis-Browne and a functioning penis was formed using a skin flap plasty supported by rib cartilage or an artificial prosthesis.", "contents": "[Operative theatment of male pseudohermaphroditism (author's transl)]. The operative treatment of two patients with male pseudohermaphroditism is reported. The patients reported were girls whose psychosexual attitude indicated a male sexual change. During several procedures the vagina was removed, the urethra was extended according to the method of Denis-Browne and a functioning penis was formed using a skin flap plasty supported by rib cartilage or an artificial prosthesis."}
{"id": "PMID:1098267", "title": "[Autovenous aorto-coronary shunting].", "content": "It is emphasized that the results of aorto-coronary shunting depend on the shunt patency. It is the author's opinion that the shunt patency is influenced by many factors, among which morphological changes in the wall of the venous graft are of great importance. The technic of taking the large saphenous vein in patients, its preparation for transplantation and a selection of the venous segment for grafting are described.", "contents": "[Autovenous aorto-coronary shunting]. It is emphasized that the results of aorto-coronary shunting depend on the shunt patency. It is the author's opinion that the shunt patency is influenced by many factors, among which morphological changes in the wall of the venous graft are of great importance. The technic of taking the large saphenous vein in patients, its preparation for transplantation and a selection of the venous segment for grafting are described."}
{"id": "PMID:1098270", "title": "[Conservation of the lung in the course of 2 to 24 hours with delayed autotransplantation in the experiment].", "content": "Opportunities of preservation of the lung at +4 degrees C and in association of this temperature with hyperbaric oxygenation during 2 and 24 hours with postponed autotransplantation have been studied. In 74 experiments on dogs postponed autotransplantation of the lung was performed, including 46--with preservation during 2 hours, and 28--with preservation during 24 hours. Based on the studies carried out, it was found that in preservation of the lung during 2 hours at +4 degrees C or in association of +4 degrees C and hyperbaric oxygenation the lung could be successfully employed for postponed autotransplantation. It is felt that there is no considerable difference between the results of experiments with postponed autotransplatation of the lung in preservation during 2 and 24 hours, depending on different conditions of preservation.", "contents": "[Conservation of the lung in the course of 2 to 24 hours with delayed autotransplantation in the experiment]. Opportunities of preservation of the lung at +4 degrees C and in association of this temperature with hyperbaric oxygenation during 2 and 24 hours with postponed autotransplantation have been studied. In 74 experiments on dogs postponed autotransplantation of the lung was performed, including 46--with preservation during 2 hours, and 28--with preservation during 24 hours. Based on the studies carried out, it was found that in preservation of the lung during 2 hours at +4 degrees C or in association of +4 degrees C and hyperbaric oxygenation the lung could be successfully employed for postponed autotransplantation. It is felt that there is no considerable difference between the results of experiments with postponed autotransplatation of the lung in preservation during 2 and 24 hours, depending on different conditions of preservation."}
{"id": "PMID:1098288", "title": "Pediatric renal transplantation.", "content": "Thirty-one children received 38 kidney transplants from 22 live and 16 cadaver donors. Among the 31 patients, 25 received one transplant each, 5 received two transplants each and 1 received three transplants. Peritoneal or hemodialysis (or both) was carried out in 22 patients, with an average dialytic maintenance of 12 weeks before transplantation. Posttransplant immunosuppressive therapy included prednisone and azathioprine. Antilymphocyte globulin was administered to 33 recipients as adjunctive immunosuppressive therapy. At present, 23 patients have functioning allografts, 3 are on hemodialysis and 5 are dead. Of 22 live kidney transplants, 18 are presently functioning two months to 14 years after transplantation with an average of 36 months. Of 16 cadaver kidney transplants, 5 are presently functioning 9 to 57 months after transplantation with an average of 32 months. Actuarial live donor allograft survival for one year was 76 percent, for two years was 66 percent and for three years was 64 percent. Cadaver allograft survival was 50 percent, 40 percent and 40 percent, respectively. Complications were urologic and infection related. Of nine recipients with sustained hypertension, in six the condition was due to chronic rejection, while in one it was due to recurrence of the original disease in the allograft. Linear growth was measured in 15 children who were less than 14 years of age at the time of transplantation and in whom allografts survived more than one year. Maximum average linear growth velocity occurred during the first year after transplantation. Our experience indicates pediatric renal transplantation can be successfully used in the treatment of terminal renal failure.", "contents": "Pediatric renal transplantation. Thirty-one children received 38 kidney transplants from 22 live and 16 cadaver donors. Among the 31 patients, 25 received one transplant each, 5 received two transplants each and 1 received three transplants. Peritoneal or hemodialysis (or both) was carried out in 22 patients, with an average dialytic maintenance of 12 weeks before transplantation. Posttransplant immunosuppressive therapy included prednisone and azathioprine. Antilymphocyte globulin was administered to 33 recipients as adjunctive immunosuppressive therapy. At present, 23 patients have functioning allografts, 3 are on hemodialysis and 5 are dead. Of 22 live kidney transplants, 18 are presently functioning two months to 14 years after transplantation with an average of 36 months. Of 16 cadaver kidney transplants, 5 are presently functioning 9 to 57 months after transplantation with an average of 32 months. Actuarial live donor allograft survival for one year was 76 percent, for two years was 66 percent and for three years was 64 percent. Cadaver allograft survival was 50 percent, 40 percent and 40 percent, respectively. Complications were urologic and infection related. Of nine recipients with sustained hypertension, in six the condition was due to chronic rejection, while in one it was due to recurrence of the original disease in the allograft. Linear growth was measured in 15 children who were less than 14 years of age at the time of transplantation and in whom allografts survived more than one year. Maximum average linear growth velocity occurred during the first year after transplantation. Our experience indicates pediatric renal transplantation can be successfully used in the treatment of terminal renal failure."}
{"id": "PMID:1098290", "title": "An incubation flask to study metabolism of foreign compounds by intestinal microflora.", "content": "1. The design and procedure for use of an all-glass incubation flask which facilitates the production and maintenance of an anaerobic environment suitable for the study of drug metabolism by intestinal microflora is described. 2. Evaluation was based on monitoring available oxygen with an oxygen electrode. 3. Removal of air by the combination of replacement with hydrogen, a room temperature catalyst and a fluching technique, produced low oxygen levels comparable to vacuum systems without the need for adverse pressure changes.", "contents": "An incubation flask to study metabolism of foreign compounds by intestinal microflora. 1. The design and procedure for use of an all-glass incubation flask which facilitates the production and maintenance of an anaerobic environment suitable for the study of drug metabolism by intestinal microflora is described. 2. Evaluation was based on monitoring available oxygen with an oxygen electrode. 3. Removal of air by the combination of replacement with hydrogen, a room temperature catalyst and a fluching technique, produced low oxygen levels comparable to vacuum systems without the need for adverse pressure changes."}
{"id": "PMID:1098292", "title": "Significance of amniotic fluid phospholipid determination for the prediction of neonatal respiratory distress syndrome.", "content": "198 amniotic fluid samples, obtained from 14-42 weeks of 158 patients were assessed for amniotic fluid phospholipids (total phospholipids, lecithin, sphingomyelin, lecithin/sphingomyelin ratio). The TPLP and Lec-concentrations show significant rises in concentration after 34th week whereas the sphingomyelin concentration remains virtually constant during pregnancy. L/S ratio remains relatively constant smaller than or equal to 2 prior to 32 weeks and smaller than or equal to 3 between 32 and 34 weeks but shows then a steep rise. The amount of Lec (expressed as percentage of total phospholipid concentration) rises from 30-40% at 30 weeks to 70-80% at term whereas the amount of Sph declines from 30-40% to less than 10%. In 12 cases neonatal RDS occurred. With L/S ratio treshold values of 3 correct prediction of RDS was made in 83% of cases, whereas probability of RDS occurrence was 63%. With L/S ratio values greater 3 RDS can be excluded in 95% of cases. The corresponding figures for Lec and TPLP concentration are described too. The range of different results in the literature is discussed.", "contents": "Significance of amniotic fluid phospholipid determination for the prediction of neonatal respiratory distress syndrome. 198 amniotic fluid samples, obtained from 14-42 weeks of 158 patients were assessed for amniotic fluid phospholipids (total phospholipids, lecithin, sphingomyelin, lecithin/sphingomyelin ratio). The TPLP and Lec-concentrations show significant rises in concentration after 34th week whereas the sphingomyelin concentration remains virtually constant during pregnancy. L/S ratio remains relatively constant smaller than or equal to 2 prior to 32 weeks and smaller than or equal to 3 between 32 and 34 weeks but shows then a steep rise. The amount of Lec (expressed as percentage of total phospholipid concentration) rises from 30-40% at 30 weeks to 70-80% at term whereas the amount of Sph declines from 30-40% to less than 10%. In 12 cases neonatal RDS occurred. With L/S ratio treshold values of 3 correct prediction of RDS was made in 83% of cases, whereas probability of RDS occurrence was 63%. With L/S ratio values greater 3 RDS can be excluded in 95% of cases. The corresponding figures for Lec and TPLP concentration are described too. The range of different results in the literature is discussed."}
{"id": "PMID:1098293", "title": "[The amniotic infection syndrome and premature rupture of the amnion. Manifest and threatening unspecific intra-uterine infections of the last third of pregnancy (author's transl)].", "content": "In the last third of pregnancy the unspecific bacterial diseases of placenta, umbilical cord and membranes differ from those in earlier months [154; 155; 85]. There is a parallel change in the localization of fetal organs affected. Pathologic anatomical clarification of this led to the conceptual definition of the rare extra-amnial placental-fetal path of infection on the one hand and of primary intra-amnial infection with secondary chorion-amnionitis on the other hand and to identification of the amniotic infection syndrome as a typical entity at the end of pregnancy [19]. While the amnionic sac is closed, unspecific fetal inflammation is rare. Generally this happens only after rupture of the membranes. Since the amniotic infection syndrome often produces only minor clinical symptoms and since fetal infection probably starts early, one searched for means of assessing the risk of infection independently from symptoms. In comparative series of investigations of pregnancies with and without premature rupture of the membranes, cases of neonatal death from infection were preceded by signs of inflammation in placenta, umbilical cord and membranes. Together with the fetal and maternal infections they depended on the length of time between rupture and the onset of labor. pns. The data now available give a clear picture of the development of the inflammatory processes in mature and premature children and suffice for the assessment of fetal and maternal risks.", "contents": "[The amniotic infection syndrome and premature rupture of the amnion. Manifest and threatening unspecific intra-uterine infections of the last third of pregnancy (author's transl)]. In the last third of pregnancy the unspecific bacterial diseases of placenta, umbilical cord and membranes differ from those in earlier months [154; 155; 85]. There is a parallel change in the localization of fetal organs affected. Pathologic anatomical clarification of this led to the conceptual definition of the rare extra-amnial placental-fetal path of infection on the one hand and of primary intra-amnial infection with secondary chorion-amnionitis on the other hand and to identification of the amniotic infection syndrome as a typical entity at the end of pregnancy [19]. While the amnionic sac is closed, unspecific fetal inflammation is rare. Generally this happens only after rupture of the membranes. Since the amniotic infection syndrome often produces only minor clinical symptoms and since fetal infection probably starts early, one searched for means of assessing the risk of infection independently from symptoms. In comparative series of investigations of pregnancies with and without premature rupture of the membranes, cases of neonatal death from infection were preceded by signs of inflammation in placenta, umbilical cord and membranes. Together with the fetal and maternal infections they depended on the length of time between rupture and the onset of labor. pns. The data now available give a clear picture of the development of the inflammatory processes in mature and premature children and suffice for the assessment of fetal and maternal risks."}
{"id": "PMID:1098294", "title": "Determination of serum nucleotidase with cytidine monophosphate as substrate, (I).", "content": "This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity.", "contents": "Determination of serum nucleotidase with cytidine monophosphate as substrate, (I). This paper deals with a new method for the determination of serum nucleotidase (EC 3.1.3.5). The assay is performed with cytidine-5'-monophosphate as substrate, followed by deamination of the generated cytidine. The principle of the method and the determination of the liberated ammonia by the Berthelot indophenol-reaction are comparable to the Persijn--van der Slik method in which adenosine-5'-monophosphate is used as substrate. The correlation between the results obtained with these two methods was found to be good; the new method has the advantage of higher sensitivity."}
{"id": "PMID:1098295", "title": "[Themistocles Gluck (1853-1942), pioneer in endoprosthetics].", "content": "There ist reported about the surgeon Prof. Themistocles Gluck, who first invented endoprostheses from ivory in 1890 at Berlin. So we believe, that he was the pioneer in endoprosthetics.", "contents": "[Themistocles Gluck (1853-1942), pioneer in endoprosthetics]. There ist reported about the surgeon Prof. Themistocles Gluck, who first invented endoprostheses from ivory in 1890 at Berlin. So we believe, that he was the pioneer in endoprosthetics."}
{"id": "PMID:1098296", "title": "[Results of treatment of congenital hip dislocation by extensionrepositioning and Hanausek-retention].", "content": "In the past 4 years all our patients below the age of three years, suffering from luxation or dysplasia of the hipjoint, were treated only conservatively. For repositioning we used a modified overhead-extension (Extensionsreposition). The \"Hanausek-Apparat\" was used only for retention. By this method all luxations  could be reponed. Because of the low rate of re-dislocations and femur-head-nekroses, which decreased significantly from 23 per cent to 3.9 per cent, our method proved to be superior to manual repositioning under general anaesthesia, plaster-extension or early operation.", "contents": "[Results of treatment of congenital hip dislocation by extensionrepositioning and Hanausek-retention]. In the past 4 years all our patients below the age of three years, suffering from luxation or dysplasia of the hipjoint, were treated only conservatively. For repositioning we used a modified overhead-extension (Extensionsreposition). The \"Hanausek-Apparat\" was used only for retention. By this method all luxations  could be reponed. Because of the low rate of re-dislocations and femur-head-nekroses, which decreased significantly from 23 per cent to 3.9 per cent, our method proved to be superior to manual repositioning under general anaesthesia, plaster-extension or early operation."}
{"id": "PMID:1098297", "title": "[The personality of children with weak posture].", "content": "An attempt is made on two groups of children with weak posture and of normal children aged 8 to 10 years to demonstrate a statistical link between fibrous tissue-weakness and certain psychological characteristics. In tests and observations of experiences and behavior of children one finds: Compared with the controls the children  showing symptoms suffer psychosomatic changes. The cause is held to be not just increased psychomotor clumsiness, but their behavior pattern is essentially due to psychological pressure of their environment (parents, school, friends etc). This leads to: reduction self-confidence, increased experience of failure, hypercompensatory view of the self with strong criticism of others and again physical dysfunction. To offer treatment is not enough. We have to demand a spread of information, cooperative efforts of teachers , school-physicians and parents and education by team-work.", "contents": "[The personality of children with weak posture]. An attempt is made on two groups of children with weak posture and of normal children aged 8 to 10 years to demonstrate a statistical link between fibrous tissue-weakness and certain psychological characteristics. In tests and observations of experiences and behavior of children one finds: Compared with the controls the children  showing symptoms suffer psychosomatic changes. The cause is held to be not just increased psychomotor clumsiness, but their behavior pattern is essentially due to psychological pressure of their environment (parents, school, friends etc). This leads to: reduction self-confidence, increased experience of failure, hypercompensatory view of the self with strong criticism of others and again physical dysfunction. To offer treatment is not enough. We have to demand a spread of information, cooperative efforts of teachers , school-physicians and parents and education by team-work."}
{"id": "PMID:1098322", "title": "Studies on the energetic metabolism of Shigella flexneri and Escherichia coli hybrids devoid of penetration ability. II. Oxygen uptake and dehydrogenase activity.", "content": "Oxygen uptake and dehydrogenase activity are studied with lac+ kcpminus hybrids of Shigella flexneri, being avirulent to the eyes of guinea pigs, because of having lost ability for penetration in epithelial cells. These strains were obtained by hybridization of the virulent strain Shigella flexneri 222 5 a with the Hfr strain Escherichia coli K-12 P4x. It is established that lac+ kcp minus hybrids are characterized by significant decrease of respiratory activity. This decrease is outlined as a tendency studying O2 uptake and is extremely well expressed studying the dehydrogenase activity. The tests being performed with these hybrids show that the loss of virulence and connected with it the loss of the ability for penetration is related to a decrease of the respiratory activity of these bacteria which supply a part of the energy needed for their viability.", "contents": "Studies on the energetic metabolism of Shigella flexneri and Escherichia coli hybrids devoid of penetration ability. II. Oxygen uptake and dehydrogenase activity. Oxygen uptake and dehydrogenase activity are studied with lac+ kcpminus hybrids of Shigella flexneri, being avirulent to the eyes of guinea pigs, because of having lost ability for penetration in epithelial cells. These strains were obtained by hybridization of the virulent strain Shigella flexneri 222 5 a with the Hfr strain Escherichia coli K-12 P4x. It is established that lac+ kcp minus hybrids are characterized by significant decrease of respiratory activity. This decrease is outlined as a tendency studying O2 uptake and is extremely well expressed studying the dehydrogenase activity. The tests being performed with these hybrids show that the loss of virulence and connected with it the loss of the ability for penetration is related to a decrease of the respiratory activity of these bacteria which supply a part of the energy needed for their viability."}
{"id": "PMID:1098323", "title": "[The anodic-moving thermolabile antigen (ATA) of gram-negative bacteria: its occurrence in rough mutants of shigella flexneri and Escherichia coli(author's transl)].", "content": "The thermolabile antigen (ATA) moving to the anode, discovered by us (9) and also described by LARSSON et al. (8), could be detected in Ra-, Rb-, Re- and Rd-mutants of Sh. flexneri and, in smaller amounts, in Rd- and Re- mutants of E. coli O8:K27:H. So it proves to be different from KUNIN'S common antigen (7). Our results lead to the conclusion that ATA is located in a more inner part of the bacterial cell wall and is fixed by noncovalent linkages.", "contents": "[The anodic-moving thermolabile antigen (ATA) of gram-negative bacteria: its occurrence in rough mutants of shigella flexneri and Escherichia coli(author's transl)]. The thermolabile antigen (ATA) moving to the anode, discovered by us (9) and also described by LARSSON et al. (8), could be detected in Ra-, Rb-, Re- and Rd-mutants of Sh. flexneri and, in smaller amounts, in Rd- and Re- mutants of E. coli O8:K27:H. So it proves to be different from KUNIN'S common antigen (7). Our results lead to the conclusion that ATA is located in a more inner part of the bacterial cell wall and is fixed by noncovalent linkages."}
{"id": "PMID:1098324", "title": "Occurrence of megasphaera elsdenii in faecal samples of young pigs.", "content": "Important numbers of Megasphaera elsdenii, known as a normal inhabitant of the rumen of cattle and sheep were recently cultured from intestinal contents of man and pigs. Studying the occurrence of this organism in faecal contents of pigs, we paid attention to the use of a strict anaerobic isolating and counting technique. It was demonstrated that Megasphaera elsdenii is a strict anaerobic organism. Exposure to air during 15 minutes caused an important decrease of the colony count. Comparison of the biochemical properties of some isolates with the data of other investigators is given. Pigs isolates were nearly similar to rumen strains.", "contents": "Occurrence of megasphaera elsdenii in faecal samples of young pigs. Important numbers of Megasphaera elsdenii, known as a normal inhabitant of the rumen of cattle and sheep were recently cultured from intestinal contents of man and pigs. Studying the occurrence of this organism in faecal contents of pigs, we paid attention to the use of a strict anaerobic isolating and counting technique. It was demonstrated that Megasphaera elsdenii is a strict anaerobic organism. Exposure to air during 15 minutes caused an important decrease of the colony count. Comparison of the biochemical properties of some isolates with the data of other investigators is given. Pigs isolates were nearly similar to rumen strains."}
{"id": "PMID:1098325", "title": "In vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. I. influence on the composition of the flora.", "content": "In vitro fermentations with the gastro-intestinal flora of 18 treated piglets (Control, Virginiamycin, Spiramycin) were carried out to study the influence of Nutritional doses of Virginiamycin and Spiramycin on the composition of the microflora. Each total flora was used for three different incubations: Control, 50 ppm Virginiamycin, 50 ppm Spiramycin. To obtain comparable results with experiments in vivo, an artificial fermentation medium was made on the basis of several chemical analyses of intestinal contents. The treatments with nutritional doses in vivo had little influence on the different in vitro fermentations. Addition of Spiramycin during incubation was of little influence on the growth and the composition of the flora. Virginiamycin on the other hand caused marked changes in the gastro-intestinal microflora, such as a decrease of the total growth and a decrease in the number of Lactobacilli and Enterococci, combined with a slight increase of the Gram negative aerobic population.", "contents": "In vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. I. influence on the composition of the flora. In vitro fermentations with the gastro-intestinal flora of 18 treated piglets (Control, Virginiamycin, Spiramycin) were carried out to study the influence of Nutritional doses of Virginiamycin and Spiramycin on the composition of the microflora. Each total flora was used for three different incubations: Control, 50 ppm Virginiamycin, 50 ppm Spiramycin. To obtain comparable results with experiments in vivo, an artificial fermentation medium was made on the basis of several chemical analyses of intestinal contents. The treatments with nutritional doses in vivo had little influence on the different in vitro fermentations. Addition of Spiramycin during incubation was of little influence on the growth and the composition of the flora. Virginiamycin on the other hand caused marked changes in the gastro-intestinal microflora, such as a decrease of the total growth and a decrease in the number of Lactobacilli and Enterococci, combined with a slight increase of the Gram negative aerobic population."}
{"id": "PMID:1098326", "title": "[Nocardia pellegrini: III. An attempt of phage typing(author's transl)].", "content": "The typing of Nocardia pellegrini with different Nocardia-phages was studied. The investigation included 25 Nocardia pellegrini strains, 19 strains of different Nocardia species and also strains of different species of mycobacteria. It could be stated that phages typical for Nocardia pellegrini were also active against other fast fragmentating Nocardia species, such as Nocardia corallina or Nocardia rubra. However, they did not attack Nocardia asteroides or strains of mycobacteria. Phages typical for mycobacteria or for Nocardia asteroides did not lysate the fast fragmentating nocardia (Table 1 and 2). Mutants of one Nocardia pellegrini phage could be isolated which showed a broader host range than the parent phage (Table 1 and 2). Some lysogenic strains of Nocardia pellegrini with a changed phage sensitivity could be isolated after action of the phages (Table 3). The taxonomical position of Nocardia pellegrini and Nocardia rubra is discussed.", "contents": "[Nocardia pellegrini: III. An attempt of phage typing(author's transl)]. The typing of Nocardia pellegrini with different Nocardia-phages was studied. The investigation included 25 Nocardia pellegrini strains, 19 strains of different Nocardia species and also strains of different species of mycobacteria. It could be stated that phages typical for Nocardia pellegrini were also active against other fast fragmentating Nocardia species, such as Nocardia corallina or Nocardia rubra. However, they did not attack Nocardia asteroides or strains of mycobacteria. Phages typical for mycobacteria or for Nocardia asteroides did not lysate the fast fragmentating nocardia (Table 1 and 2). Mutants of one Nocardia pellegrini phage could be isolated which showed a broader host range than the parent phage (Table 1 and 2). Some lysogenic strains of Nocardia pellegrini with a changed phage sensitivity could be isolated after action of the phages (Table 3). The taxonomical position of Nocardia pellegrini and Nocardia rubra is discussed."}
{"id": "PMID:1098327", "title": "Studies on the immunizing capacity of orally administered particulate antigens. II. Production of immunological memory in germ-free mice by orally administered sheep erythrocytes.", "content": "A study was perfromed to find out, whether or not the oral administration of sheep erythrocytes results in a general primary immune reaction as well as in effective priming for the secondary response in both conventional and germ-free NMRI mice. Whereas negative results were obtained with conventionally held mice, five oral applications of 0.3 ml of a 60% suspension of sheep erythrocytes to germ-free mice, each dose separated by an interval of 24 hr, resulted in a general primary immune response both at the cellular and humoral levels. When such pretreated mice were given an i.p. injection of 4 times 10(8) sheep erythrocytes as a secondary antigenic stimulus 32 days after the last of the five oral applications, the subsequent response was characterized by the predominant development of 7S hemolysin-producing spleen cells. This evidently indicates that effective priming for the secondary response has taken place by the orally administered antigen.", "contents": "Studies on the immunizing capacity of orally administered particulate antigens. II. Production of immunological memory in germ-free mice by orally administered sheep erythrocytes. A study was perfromed to find out, whether or not the oral administration of sheep erythrocytes results in a general primary immune reaction as well as in effective priming for the secondary response in both conventional and germ-free NMRI mice. Whereas negative results were obtained with conventionally held mice, five oral applications of 0.3 ml of a 60% suspension of sheep erythrocytes to germ-free mice, each dose separated by an interval of 24 hr, resulted in a general primary immune response both at the cellular and humoral levels. When such pretreated mice were given an i.p. injection of 4 times 10(8) sheep erythrocytes as a secondary antigenic stimulus 32 days after the last of the five oral applications, the subsequent response was characterized by the predominant development of 7S hemolysin-producing spleen cells. This evidently indicates that effective priming for the secondary response has taken place by the orally administered antigen."}
{"id": "PMID:1098328", "title": "[Laboratory procedures in adenoviruses. II. Sensitivity of various cell cultures as determined by endpoint titration (author's transl)].", "content": "The sensitivity of human embryonic kidney (HEK), human amnion (HAm) and HeLa cells for human adenoviruses was investigated by means of endpoint titrations of 13 prototype strains and numerous original specimens from patients. Prototypes and original specimens showed the same behavior. Adenoviruses of subgrouppp III produced nearly identical titers in all 3 cell cultures, while for subgroup I HAm cells showed a 10- to 100-fold lower titer. On the other hand, HAm cells are nearly as sensitive as HEK cells for type 8, whereas HeLa cells are less sensitive. For other viruses of subgroup II and for subgrou IV, HAm (for type 12 also HeLa) cells are less sensitive than HEK cells. Out of three HeLa cell strains tested the Bristol strain was inferior to the other two in its sensitivity. Concerning the speed of the CPE development, HEK cells are superior for all types, the difference being greatest for subgroup I towards HAm and HeLa and for type 8 towards HeLa cells. The length of incubation necessary for the appearance of CPE even with minimal amounts of virus is 40 days for type 8 in all kinds of cell cultures, whereas for adenoviruses of sub-groups I and III, which are predominantly isolated in the routine laboratory, 25 days for HEK and 30 days for HAm and HeLa cells may suffice in most cases. When endpoint titrations are compared with titers obtained by immunofluorescence, it appears that this technique may be applied as a screening method; thus long incubation periods of negative specimens can be saved. The amounts of infectious virus present in original specimens are reported; the highest quantity was found in specimens containing subgroup I viruses.", "contents": "[Laboratory procedures in adenoviruses. II. Sensitivity of various cell cultures as determined by endpoint titration (author's transl)]. The sensitivity of human embryonic kidney (HEK), human amnion (HAm) and HeLa cells for human adenoviruses was investigated by means of endpoint titrations of 13 prototype strains and numerous original specimens from patients. Prototypes and original specimens showed the same behavior. Adenoviruses of subgrouppp III produced nearly identical titers in all 3 cell cultures, while for subgroup I HAm cells showed a 10- to 100-fold lower titer. On the other hand, HAm cells are nearly as sensitive as HEK cells for type 8, whereas HeLa cells are less sensitive. For other viruses of subgroup II and for subgrou IV, HAm (for type 12 also HeLa) cells are less sensitive than HEK cells. Out of three HeLa cell strains tested the Bristol strain was inferior to the other two in its sensitivity. Concerning the speed of the CPE development, HEK cells are superior for all types, the difference being greatest for subgroup I towards HAm and HeLa and for type 8 towards HeLa cells. The length of incubation necessary for the appearance of CPE even with minimal amounts of virus is 40 days for type 8 in all kinds of cell cultures, whereas for adenoviruses of sub-groups I and III, which are predominantly isolated in the routine laboratory, 25 days for HEK and 30 days for HAm and HeLa cells may suffice in most cases. When endpoint titrations are compared with titers obtained by immunofluorescence, it appears that this technique may be applied as a screening method; thus long incubation periods of negative specimens can be saved. The amounts of infectious virus present in original specimens are reported; the highest quantity was found in specimens containing subgroup I viruses."}
{"id": "PMID:1098330", "title": "[Qualitative und quantitative salmonella investigations and their hygienic valuation in connection with the E. coli titre, demonstrated with examples from the coastal waters of Kiel bight (Western Baltic Sea) (author's transl)].", "content": "Qualitative salmonella investigations and E. coli titre determinations were carried out in about 4,000 water samples taken from the coastal region of Kiel Bight (Western Baltic) in 1972 and 1973, and evaluated in connection with epidemiological data. Further, about 100 samples were determined quantitatively by the MPN method and set in relation to the E. coli titre. The significance to be attributed to positive salmonella findings particularly in relation to the assessment of risk of infection and as impurity indicators was investigated with reference to this material. A comparison of salmonella findings and E. coli titre showed that with increasing E. coli contamination the proportion of positive salmonella findings also increased (paraboloid curve). Salmonella frequencies and the frequency with which the limiting values of the E. coli titre is exceeded run approximately parallel; the frequency of exceeding a limiting value of E. coli titre of 1.0 and the frequency of salmonella (determination in 100 ml) in sewage laden areas largely coincide. The quantitative salmonella investigations (MPN method) also showed that up to an E. coli titre of 1.0 extremely low salmonella counts only are to be expected (on the average, less than 2/100 ml). With an E. coli titre of 0.1, a marked increase can be seen. A limiting value of 1.0 is proposed for the E. coli titre in coastal waters. Epidemiological data have also shown that an increased risk of infection could not be deduced from positive salmonella findings. According to the present investigations, the value of the information obtained from salmonella findings is small compared with the E. coli titre. Considering the great expense of laboratory investigations, tests for salmonella should be dispensed with in routine examination of water.", "contents": "[Qualitative und quantitative salmonella investigations and their hygienic valuation in connection with the E. coli titre, demonstrated with examples from the coastal waters of Kiel bight (Western Baltic Sea) (author's transl)]. Qualitative salmonella investigations and E. coli titre determinations were carried out in about 4,000 water samples taken from the coastal region of Kiel Bight (Western Baltic) in 1972 and 1973, and evaluated in connection with epidemiological data. Further, about 100 samples were determined quantitatively by the MPN method and set in relation to the E. coli titre. The significance to be attributed to positive salmonella findings particularly in relation to the assessment of risk of infection and as impurity indicators was investigated with reference to this material. A comparison of salmonella findings and E. coli titre showed that with increasing E. coli contamination the proportion of positive salmonella findings also increased (paraboloid curve). Salmonella frequencies and the frequency with which the limiting values of the E. coli titre is exceeded run approximately parallel; the frequency of exceeding a limiting value of E. coli titre of 1.0 and the frequency of salmonella (determination in 100 ml) in sewage laden areas largely coincide. The quantitative salmonella investigations (MPN method) also showed that up to an E. coli titre of 1.0 extremely low salmonella counts only are to be expected (on the average, less than 2/100 ml). With an E. coli titre of 0.1, a marked increase can be seen. A limiting value of 1.0 is proposed for the E. coli titre in coastal waters. Epidemiological data have also shown that an increased risk of infection could not be deduced from positive salmonella findings. According to the present investigations, the value of the information obtained from salmonella findings is small compared with the E. coli titre. Considering the great expense of laboratory investigations, tests for salmonella should be dispensed with in routine examination of water."}
{"id": "PMID:1098331", "title": "[Bacteriological and parasitological investigations in public indoor swimming baths (author's transl)].", "content": "The importance of the public swimming bath for the dissemination of Trichomonas vaginalis is disputed. 30 samples, each of 250 ml bath water from four indoor swimming baths, were examined for trichomonads. All the samples were negative. 218 smears taken from the wet places on the heated benches and other seating accommodation in these swimming baths were cultured for trichomonads and bacteria. Trichomonas vaginalis could not be demonstrated. Fecal organisms were found in 56 places, antibiotic-sensitive staphylococci were cultured from 20 samples and Ps. aeruginosa from 8. The negative tests for trichomonads suggests that the indoor swimming bath has no epidemiological importance in the dissemination of this parasite.", "contents": "[Bacteriological and parasitological investigations in public indoor swimming baths (author's transl)]. The importance of the public swimming bath for the dissemination of Trichomonas vaginalis is disputed. 30 samples, each of 250 ml bath water from four indoor swimming baths, were examined for trichomonads. All the samples were negative. 218 smears taken from the wet places on the heated benches and other seating accommodation in these swimming baths were cultured for trichomonads and bacteria. Trichomonas vaginalis could not be demonstrated. Fecal organisms were found in 56 places, antibiotic-sensitive staphylococci were cultured from 20 samples and Ps. aeruginosa from 8. The negative tests for trichomonads suggests that the indoor swimming bath has no epidemiological importance in the dissemination of this parasite."}
{"id": "PMID:1098332", "title": "[Characteristics of capsular bacteria, isolated from children with acute intestinal diseases].", "content": "A study was made of 256 young children suffering from gastroenterocolitis, dyspepsia, food poisoning for the presence of conditionally pathogenic representatives of the Enterobacteriaceae family. Klebsiellae were isolated in 39.8% of the patients and in 22.3% of 67 healthy children. A study was made of morphological, cultural-biochemical properties and the antigenic structure in 308 strains of the Klebsiella denus. Capsules were detected by microscopic examination. Biochemical properties were determined after Kaufman, and some of the cultures proved to differ from the typical biochemical pattern described by this author. K-antigen was found in 185 klebsiella strains; of this number 99 strains belonged to Kl. pneumoniae and 86--to Kl. aerogenes. Fimbria were revealed in some of the Kl. pneumoniae and Kl. aerogenes strains. Bacteriocines were detected in 103 of 206 strains (by Abbot and Shennon's method).", "contents": "[Characteristics of capsular bacteria, isolated from children with acute intestinal diseases]. A study was made of 256 young children suffering from gastroenterocolitis, dyspepsia, food poisoning for the presence of conditionally pathogenic representatives of the Enterobacteriaceae family. Klebsiellae were isolated in 39.8% of the patients and in 22.3% of 67 healthy children. A study was made of morphological, cultural-biochemical properties and the antigenic structure in 308 strains of the Klebsiella denus. Capsules were detected by microscopic examination. Biochemical properties were determined after Kaufman, and some of the cultures proved to differ from the typical biochemical pattern described by this author. K-antigen was found in 185 klebsiella strains; of this number 99 strains belonged to Kl. pneumoniae and 86--to Kl. aerogenes. Fimbria were revealed in some of the Kl. pneumoniae and Kl. aerogenes strains. Bacteriocines were detected in 103 of 206 strains (by Abbot and Shennon's method)."}
{"id": "PMID:1098333", "title": "[Morphological changes during intestinal dysbacteriosis in rats].", "content": "Inclusion into the food of rats of living E. coli strains produced an inflammatory-destructive process in the mucose of the cecum. When a strain isolated from the patient suffering from carcinoma of the stomach was fed the changes appeared in 2 to 3 months after the beginning of the experiment and were accompanied by atrophy of the mucosa; after colibacterin administration the inflammatory process occurred later, it was less pronounced and accompanied by the repair reaction.", "contents": "[Morphological changes during intestinal dysbacteriosis in rats]. Inclusion into the food of rats of living E. coli strains produced an inflammatory-destructive process in the mucose of the cecum. When a strain isolated from the patient suffering from carcinoma of the stomach was fed the changes appeared in 2 to 3 months after the beginning of the experiment and were accompanied by atrophy of the mucosa; after colibacterin administration the inflammatory process occurred later, it was less pronounced and accompanied by the repair reaction."}
{"id": "PMID:1098334", "title": "[Experience in the use of Tebrophen for influenza prevention].", "content": "The efficacy of influenca prophylaxis by a new Soviet chemopreparation tebrophen was studied during the influenza epidemics of 1972-1973 caused by the influenza virus of A/Hong-kong/68 and A/England/72/H3N2/types. Investigations were carried out under conditions of an epidemiological experience among the organized (adult) collective bodies (4352 persons in all). The preparation was instilled intranasally in the form of a 0.25 and 1% ointment on vaseline base. The efficacy index was 2.0-2.7, and the protection index--51--60%.", "contents": "[Experience in the use of Tebrophen for influenza prevention]. The efficacy of influenca prophylaxis by a new Soviet chemopreparation tebrophen was studied during the influenza epidemics of 1972-1973 caused by the influenza virus of A/Hong-kong/68 and A/England/72/H3N2/types. Investigations were carried out under conditions of an epidemiological experience among the organized (adult) collective bodies (4352 persons in all). The preparation was instilled intranasally in the form of a 0.25 and 1% ointment on vaseline base. The efficacy index was 2.0-2.7, and the protection index--51--60%."}
{"id": "PMID:1098335", "title": "[Dynamics of dysenteric diseases, caused by Sh. sonnei of different enzymatic and conditional colicinogenotypes, in Kharkov in 1965-1972].", "content": "Results of studying the enzymatic (by maltose, rhamnose and xylose) properties of 9277 freshly-located Sh. sonnei strains isolated in Kharkov in 1965-1972 showed that they were referred to 14 enzymatic types (a, b, c, d, e, f, g, k, l, m, n, o, p, q). The strains under study were additionally--within the range of each of the enzymatic type-divided into 8 conditioned coli phi,cinogen types (I-VIII) by their sensitivity to colicines of the indicator E. coli, K12 and B cultures. Subdivision of the strains with the use of the two mentioned tests permitted to establish the prevalence among the Sh. sonnei strains isolated during the individual years of definite conditioned enzymatic-colicinogenic types. During the period observed group diseases were caused by Sh. sonnei of only some of the enzymatic-colicinogenic types-aII, aVI, dI, dII, dVI, etc. Three enzymatic types of Sh. sonnei-a, d and k-dominated in Kharkov beginning from 1969.", "contents": "[Dynamics of dysenteric diseases, caused by Sh. sonnei of different enzymatic and conditional colicinogenotypes, in Kharkov in 1965-1972]. Results of studying the enzymatic (by maltose, rhamnose and xylose) properties of 9277 freshly-located Sh. sonnei strains isolated in Kharkov in 1965-1972 showed that they were referred to 14 enzymatic types (a, b, c, d, e, f, g, k, l, m, n, o, p, q). The strains under study were additionally--within the range of each of the enzymatic type-divided into 8 conditioned coli phi,cinogen types (I-VIII) by their sensitivity to colicines of the indicator E. coli, K12 and B cultures. Subdivision of the strains with the use of the two mentioned tests permitted to establish the prevalence among the Sh. sonnei strains isolated during the individual years of definite conditioned enzymatic-colicinogenic types. During the period observed group diseases were caused by Sh. sonnei of only some of the enzymatic-colicinogenic types-aII, aVI, dI, dII, dVI, etc. Three enzymatic types of Sh. sonnei-a, d and k-dominated in Kharkov beginning from 1969."}
{"id": "PMID:1098336", "title": "[Relation of the immune response in rats to the state of the lysosomes and intensity of protein synthesis].", "content": "In stimulation of an immune response to salmonellae in rats by dibasol and methyluracil and its depression by chlorophos and an amine salt of 2,4-dichlorphenoacetic acid it was found that the intensity of the immune response depended more on the level of protein biosynthesis than on the state of lysosomes at the period preceding the immunization.", "contents": "[Relation of the immune response in rats to the state of the lysosomes and intensity of protein synthesis]. In stimulation of an immune response to salmonellae in rats by dibasol and methyluracil and its depression by chlorophos and an amine salt of 2,4-dichlorphenoacetic acid it was found that the intensity of the immune response depended more on the level of protein biosynthesis than on the state of lysosomes at the period preceding the immunization."}
{"id": "PMID:1098337", "title": "[Induction of autologous tissue disorders in animals tolerant to cross-reacting microbial antigens].", "content": "Injection to newborn C3H mice of streptococcus, group C, or C. albicans vaccines prevented in adult mice induction of the skin autotransplant affection caused by the immunization of these animals with the mentioned microbial vaccines. In response to the immunization with the streptococcus or monilia vaccines the tolerant mice displayed a reduction in the number of cells reacting with the microbial vaccines in the immunoadhesion and blasttransformation reactions; their cytotoxic activity in respect to the syngenous target cells was also diminished.", "contents": "[Induction of autologous tissue disorders in animals tolerant to cross-reacting microbial antigens]. Injection to newborn C3H mice of streptococcus, group C, or C. albicans vaccines prevented in adult mice induction of the skin autotransplant affection caused by the immunization of these animals with the mentioned microbial vaccines. In response to the immunization with the streptococcus or monilia vaccines the tolerant mice displayed a reduction in the number of cells reacting with the microbial vaccines in the immunoadhesion and blasttransformation reactions; their cytotoxic activity in respect to the syngenous target cells was also diminished."}
{"id": "PMID:1098338", "title": "[Detection of atoxigenic variants in Cl. botulinum type A and B cultures exposed to different treatments].", "content": "A possibility of detection of atoxigenic clones in the cultures of Cl. botulinum, types A and B, under conditions of treatment with acridine strains, UV-irradiation and heating of spores was studied. Of the 10 strains investigated, atoxigenic variants were obtained only in 3, belonging to type B; the frequency of formation of atoxigenic clones under the effect of ethidium bronide constituted 36--45.7%, and of the UV-irradiation-4.2%; heating of spores proved to be ineffective. A frequent loss of the toxigenicity sign coincided with the view on the elimination of the episomal determinant of the toxin-formation, not with the mutational nature-of this change. An association between the differences in stability of retention of the toxigenicity signs in various strains and types of Cl. botulinum and possible differences of the corresponding genetic determinants of the toxin formation is discussed.", "contents": "[Detection of atoxigenic variants in Cl. botulinum type A and B cultures exposed to different treatments]. A possibility of detection of atoxigenic clones in the cultures of Cl. botulinum, types A and B, under conditions of treatment with acridine strains, UV-irradiation and heating of spores was studied. Of the 10 strains investigated, atoxigenic variants were obtained only in 3, belonging to type B; the frequency of formation of atoxigenic clones under the effect of ethidium bronide constituted 36--45.7%, and of the UV-irradiation-4.2%; heating of spores proved to be ineffective. A frequent loss of the toxigenicity sign coincided with the view on the elimination of the episomal determinant of the toxin-formation, not with the mutational nature-of this change. An association between the differences in stability of retention of the toxigenicity signs in various strains and types of Cl. botulinum and possible differences of the corresponding genetic determinants of the toxin formation is discussed."}
{"id": "PMID:1098340", "title": "Alkylated cytosine nucleosides: substrate and inhibitor properties in enzymatic deamination.", "content": "Cytosine nucleoside deaminase (EC 3.5.4.5) from Salmonella typhimurium LT2 catalyses the deamination of ribo-, deoxyribo- and arabinosyl nucleosides of cytosine alkylated at the C-5, but not at the N3 or exocyclic N4, of the pyrimidine ring. The enzyme was inert towards analogues etherified at the 3'-OH and 5'-OH of the sugar ring; it was active against the 2'-O-methyl derivative of cytidine, but not arabinosycytosine. The N4-and 5'-O-alkyl non-substrate analogues competitively inhibited deamination of deoxycytidine and arabinosylcytosine, the most inhibitory being 5'-O-methylarabinosylcytosine. The alpha anomer of 5'-ethyldeoxycytidine, the 2,2'-anhydro derivative of cytidine, and the 3'-O-alkyl derivatives were neither substrates nor inhibitors. The presence of cytidine deaminase was demonstrated in both granulocytes and lymphocytes from human peripheral blood. The specificity of this enzyme differed significantly from that of the bacterial enzyme, a finding of some relevance in relation to the frequently encountered intracellular deamination of therapeutically active arabinosylcytosine to the inactive arabinosyluracil.", "contents": "Alkylated cytosine nucleosides: substrate and inhibitor properties in enzymatic deamination. Cytosine nucleoside deaminase (EC 3.5.4.5) from Salmonella typhimurium LT2 catalyses the deamination of ribo-, deoxyribo- and arabinosyl nucleosides of cytosine alkylated at the C-5, but not at the N3 or exocyclic N4, of the pyrimidine ring. The enzyme was inert towards analogues etherified at the 3'-OH and 5'-OH of the sugar ring; it was active against the 2'-O-methyl derivative of cytidine, but not arabinosycytosine. The N4-and 5'-O-alkyl non-substrate analogues competitively inhibited deamination of deoxycytidine and arabinosylcytosine, the most inhibitory being 5'-O-methylarabinosylcytosine. The alpha anomer of 5'-ethyldeoxycytidine, the 2,2'-anhydro derivative of cytidine, and the 3'-O-alkyl derivatives were neither substrates nor inhibitors. The presence of cytidine deaminase was demonstrated in both granulocytes and lymphocytes from human peripheral blood. The specificity of this enzyme differed significantly from that of the bacterial enzyme, a finding of some relevance in relation to the frequently encountered intracellular deamination of therapeutically active arabinosylcytosine to the inactive arabinosyluracil."}
{"id": "PMID:1098341", "title": "Advantage of defined tissue slices against minced samples in biochemical in vitro investigations of brain cortex.", "content": "A cutting device for the preparation of brain cortex slices with a regular cylindric shape and a defined thickness is described. Determinations of the glucose and the electrolyte metabolism and the reactions of both to substances' additions were made with minced brain and with brain slices of different thichknes. The comparison of the methods and of the results makes evident that experimentation with slices has several advantages.", "contents": "Advantage of defined tissue slices against minced samples in biochemical in vitro investigations of brain cortex. A cutting device for the preparation of brain cortex slices with a regular cylindric shape and a defined thickness is described. Determinations of the glucose and the electrolyte metabolism and the reactions of both to substances' additions were made with minced brain and with brain slices of different thichknes. The comparison of the methods and of the results makes evident that experimentation with slices has several advantages."}
{"id": "PMID:1098342", "title": "[In-vitro cultivated cells of smooth muscular organs. 1. Cultivation of isolated rat bladder cells].", "content": "Cells obtained from the rats urinary bladder by enzymatic treatment were cultivated on glass surfaces in completely synthetic nutrient media supplemented with 10% calf serum. The occurrence of smooth muscle cells, in addition to fibroblasts and multinuclear giant cells, in the monolayer cultures obtained is postulated.", "contents": "[In-vitro cultivated cells of smooth muscular organs. 1. Cultivation of isolated rat bladder cells]. Cells obtained from the rats urinary bladder by enzymatic treatment were cultivated on glass surfaces in completely synthetic nutrient media supplemented with 10% calf serum. The occurrence of smooth muscle cells, in addition to fibroblasts and multinuclear giant cells, in the monolayer cultures obtained is postulated."}
{"id": "PMID:1098343", "title": "Application of histoacryl-N-blau and Surgicel in experimental pancreas injuries and resections.", "content": "A new method has been worked out for the treatment of experimental pancreas injuries and resections. The surfaces were coated with SurgicelR and the coating was fixed with the adhesive Histoacryl-N-Blau. Use of these two completely absorbable substances prevented the complications caused by sutures. The results confirmed by histological and laboratory tests have been satisfactory, hence the method appears to be clinically applicable.", "contents": "Application of histoacryl-N-blau and Surgicel in experimental pancreas injuries and resections. A new method has been worked out for the treatment of experimental pancreas injuries and resections. The surfaces were coated with SurgicelR and the coating was fixed with the adhesive Histoacryl-N-Blau. Use of these two completely absorbable substances prevented the complications caused by sutures. The results confirmed by histological and laboratory tests have been satisfactory, hence the method appears to be clinically applicable."}
{"id": "PMID:1098344", "title": "Superior mesenteric blood flow in man studied with a dye-dilution technique.", "content": "The superior mesenteric blood flow in the post-absorptive state was studied with a dye-dilution technique using indocyanine green in 37 patients with apparently normal small bowel function. All the patients except 2 had been, or were to be, treated because of malignant disease, mostly rectal carcinoma. The investigations were performed in connection with portography. The dye was injected into the superior mesenteric artery as a single injection during continuous sampling of blood from the superior mesenteric vein, catheterized through the reopened umbilical vein. The blood flow was calculated from the dye-dilution curves obtained with a modified Stewart-Hamilton formula. As recirculating dye appeared in the later part of the dye-dilution curves, the appearance of the recirculating dye was timed in 4 patients. The hepatic extraction of the amount of dye usually injected, 1.25 mg, was 60%. The superior mesenteric blood flow averaged 706 ml/min (397 ml/min/m-2 body surface) or 12% of the cardiac output. The appearance time, mean transit time, vascular resistance and vascular volume were also determined.", "contents": "Superior mesenteric blood flow in man studied with a dye-dilution technique. The superior mesenteric blood flow in the post-absorptive state was studied with a dye-dilution technique using indocyanine green in 37 patients with apparently normal small bowel function. All the patients except 2 had been, or were to be, treated because of malignant disease, mostly rectal carcinoma. The investigations were performed in connection with portography. The dye was injected into the superior mesenteric artery as a single injection during continuous sampling of blood from the superior mesenteric vein, catheterized through the reopened umbilical vein. The blood flow was calculated from the dye-dilution curves obtained with a modified Stewart-Hamilton formula. As recirculating dye appeared in the later part of the dye-dilution curves, the appearance of the recirculating dye was timed in 4 patients. The hepatic extraction of the amount of dye usually injected, 1.25 mg, was 60%. The superior mesenteric blood flow averaged 706 ml/min (397 ml/min/m-2 body surface) or 12% of the cardiac output. The appearance time, mean transit time, vascular resistance and vascular volume were also determined."}
{"id": "PMID:1098345", "title": "Superior mesenteric blood flow in man following injection of bradykinin and vasopressin into the superior mesenteric artery.", "content": "The superior mesenteric blood flow was studied with a dye-dilution technique after catheterization of the superior mesenteric artery and vein. The investigation was performed in connection with portography in 22 patients with apparently normal small bowel function. Intra-arterial injection of 5, 10 or 20 mug bradykinin was followed within one minute by an increase, on the average, of 114, 176 and 223% respectively, in the superior mesenteric blood flow. The blood flow was dose-dependent in this range. The estimated vascular resistance decreased by 52, 61 and 67%, respectively. The portal venous pressure was increased slightly after intra-arterial injection, but the pressure was unchanged after intra-portal injection. Intra-arterial injection of bradykinin causes a highly improved venous phase at superior mesenteric angiography. This may be due not only to the increased flow but to some extent also to increased capillary permeability produced by bradykinin. Intra-arterial injection of 0.125 and 0.250 IU of vasopressin decreased the superior mesenteric blood flow by 53 and 54%, respectively, within 3 minutes of the injection. The dye-dilution method used was not applicable to blood flow below a level of about 200 ml/min. Continuous infusion of 0.05 IU/min decreased the superior mesenteric blood flow by, on an average, 58%. The portal venous pressure was decreased by 25% after the intra-arterial injection, but no change in pressure was recorded after intra-portal administration. The clinical use of intra-arterial infusion of vasopressin during gastrointenstinal bleeding is discussed.", "contents": "Superior mesenteric blood flow in man following injection of bradykinin and vasopressin into the superior mesenteric artery. The superior mesenteric blood flow was studied with a dye-dilution technique after catheterization of the superior mesenteric artery and vein. The investigation was performed in connection with portography in 22 patients with apparently normal small bowel function. Intra-arterial injection of 5, 10 or 20 mug bradykinin was followed within one minute by an increase, on the average, of 114, 176 and 223% respectively, in the superior mesenteric blood flow. The blood flow was dose-dependent in this range. The estimated vascular resistance decreased by 52, 61 and 67%, respectively. The portal venous pressure was increased slightly after intra-arterial injection, but the pressure was unchanged after intra-portal injection. Intra-arterial injection of bradykinin causes a highly improved venous phase at superior mesenteric angiography. This may be due not only to the increased flow but to some extent also to increased capillary permeability produced by bradykinin. Intra-arterial injection of 0.125 and 0.250 IU of vasopressin decreased the superior mesenteric blood flow by 53 and 54%, respectively, within 3 minutes of the injection. The dye-dilution method used was not applicable to blood flow below a level of about 200 ml/min. Continuous infusion of 0.05 IU/min decreased the superior mesenteric blood flow by, on an average, 58%. The portal venous pressure was decreased by 25% after the intra-arterial injection, but no change in pressure was recorded after intra-portal administration. The clinical use of intra-arterial infusion of vasopressin during gastrointenstinal bleeding is discussed."}
{"id": "PMID:1098346", "title": "Preservation of kidneys for transplantation. Assessment of ischemic damage by renal autoperfusion.", "content": "Autoperfusion of canine kidneys was investigated as an alternative method to determinate the ischmic organ damage following various preservation procedures. As a compromise between implantation of the donor kidney and normotherimic isolated perfusion, autoperfusion is shown to offer some advantages in the study of the pathophysiology of the ischemic damaged kidney. Within 3 hours of autoperfusion, we obtained detailed information about the immediate regenerative ability of the glomerular, tubular, and vascular functions following various sorts of ischemic trauma.", "contents": "Preservation of kidneys for transplantation. Assessment of ischemic damage by renal autoperfusion. Autoperfusion of canine kidneys was investigated as an alternative method to determinate the ischmic organ damage following various preservation procedures. As a compromise between implantation of the donor kidney and normotherimic isolated perfusion, autoperfusion is shown to offer some advantages in the study of the pathophysiology of the ischemic damaged kidney. Within 3 hours of autoperfusion, we obtained detailed information about the immediate regenerative ability of the glomerular, tubular, and vascular functions following various sorts of ischemic trauma."}
{"id": "PMID:1098347", "title": "Diagnostic value of the LH-releasing hormone stimulation test in functional amenorrhea.", "content": "LH-releasing hormone (25 mug, iv.) was administered to 37 women with functional amenorrhea. In addition to the clinical classification, these patients were divided into three groups according to the basal level of serum LH. A significant correlation was found between the base-line levels of LH and the serum concentration of oestradiol plus oestrone. The absolute increment of LH after the injection of LH-RH was found to be dependent only on the base-line level of LH. Except for the patients with anorexia nervoxa, the base-line levels and the response pattern of FSH were almost the same for all three groups. From the results of this study, it was concluded that: 1. The circulating levels of oestradiol and oestrone, where derived from ovarian secretion, actually depend on the gonadotrophic stimulus. In patients with functional amenorrhea, the oestrogens do not make an independent contribution to the pituitary response to LH-RH. 2. Dysregulation of releasing hormones, whether located at the hypothalamic or suprahypothalamic level, necessarily influences the secretory capacity of the pituitary gland; long-standing deficiency of LH-RH, may finally lead to a state of pituitary \"functional\" unresponsiveness to releasing hormones. 3. In view of the excellent correlation between base-line levels of LH and the absolute increment of LH following stimulation with LH-RH, this test only accentuates the existing pituitary secretroy capacity, which can be roughly estimated from circulating levels of LH and FSH. 4. This test may be useful in distinguishing the milder cases of psychogenic amenorrhea from extreme gonadotrophic dysfunction in patients with anorexia nervosa.", "contents": "Diagnostic value of the LH-releasing hormone stimulation test in functional amenorrhea. LH-releasing hormone (25 mug, iv.) was administered to 37 women with functional amenorrhea. In addition to the clinical classification, these patients were divided into three groups according to the basal level of serum LH. A significant correlation was found between the base-line levels of LH and the serum concentration of oestradiol plus oestrone. The absolute increment of LH after the injection of LH-RH was found to be dependent only on the base-line level of LH. Except for the patients with anorexia nervoxa, the base-line levels and the response pattern of FSH were almost the same for all three groups. From the results of this study, it was concluded that: 1. The circulating levels of oestradiol and oestrone, where derived from ovarian secretion, actually depend on the gonadotrophic stimulus. In patients with functional amenorrhea, the oestrogens do not make an independent contribution to the pituitary response to LH-RH. 2. Dysregulation of releasing hormones, whether located at the hypothalamic or suprahypothalamic level, necessarily influences the secretory capacity of the pituitary gland; long-standing deficiency of LH-RH, may finally lead to a state of pituitary \"functional\" unresponsiveness to releasing hormones. 3. In view of the excellent correlation between base-line levels of LH and the absolute increment of LH following stimulation with LH-RH, this test only accentuates the existing pituitary secretroy capacity, which can be roughly estimated from circulating levels of LH and FSH. 4. This test may be useful in distinguishing the milder cases of psychogenic amenorrhea from extreme gonadotrophic dysfunction in patients with anorexia nervosa."}
{"id": "PMID:1098348", "title": "The influence of time, dose and gonadal steroids on LH-RH-induced gonadotrophin release in rats.", "content": "Hypophyseal responses to LH-releasing hormone (LH-RH) were studied in ovariectomized rats, pre-treated once with 50 mug oestradiol-3-benzoate alone or in combination with 25 mg progesterone either 16, 24, 48 or 72 h before the administration of LH-RH.  The highest serum gonadotrophin increase was detected in ovariectomized rats pre-treated with oestrogen 48 h before the LH-RH injection.  The serum LH concentration change in intact male rats was markedly lower in terms of order of magnitude than in ovariectomized, oestradiol-3-benzoate, progesterone-treated rats (\"O.Oe. P.\"-rats).  These latter test animals showed the maximal increase of serum LH concentration, depending on the dose of LH-RH, after 10 to 25 min and that of FSH after 40 min.  The dose-response curves for LH release in \"O.Oe.P.\"-rats were linear for short (10 and 15 min) and sigmoid for longer time intervals (20, 30 and 40 min) between LH-RH injection and sacrifice.  The possible reasons for this difference in both magnitude and time-lapse for maximal increase of serum LH and FSH concentration, the influence of steroid pre-treatment and the variation of the time course of serum LH concentration, depending on the dose of LH-RH, are discussed.", "contents": "The influence of time, dose and gonadal steroids on LH-RH-induced gonadotrophin release in rats. Hypophyseal responses to LH-releasing hormone (LH-RH) were studied in ovariectomized rats, pre-treated once with 50 mug oestradiol-3-benzoate alone or in combination with 25 mg progesterone either 16, 24, 48 or 72 h before the administration of LH-RH.  The highest serum gonadotrophin increase was detected in ovariectomized rats pre-treated with oestrogen 48 h before the LH-RH injection.  The serum LH concentration change in intact male rats was markedly lower in terms of order of magnitude than in ovariectomized, oestradiol-3-benzoate, progesterone-treated rats (\"O.Oe. P.\"-rats).  These latter test animals showed the maximal increase of serum LH concentration, depending on the dose of LH-RH, after 10 to 25 min and that of FSH after 40 min.  The dose-response curves for LH release in \"O.Oe.P.\"-rats were linear for short (10 and 15 min) and sigmoid for longer time intervals (20, 30 and 40 min) between LH-RH injection and sacrifice.  The possible reasons for this difference in both magnitude and time-lapse for maximal increase of serum LH and FSH concentration, the influence of steroid pre-treatment and the variation of the time course of serum LH concentration, depending on the dose of LH-RH, are discussed."}
{"id": "PMID:1098349", "title": "The sequence of pituitary responses to synthetic luteinizing hormone releasing hormone (LH-RH) throughout the normal menstrual cycle.", "content": "Thirty-one ovulatory women between 20 and 33 years of age were given 150 mug of synthetic LH-RH during different phases of the menstrual cycle. Five patients were studied during the early follicular phase (days 4-7); 10 patients during the late follicular phase (days 9-12); 6 patients during the \"LH Surge\"; 5 patients during the early luteal phase (days 14-16); 3 patients during mid-luteal phase (days 17-21); and 2 patients during late luteal phase (days 22-27). Oestrogen, progesterone, FSH and LH levels were determined from 30 min prior to LH-RH administration to 90 min thereafter in all cases. LH response to LH-RH increased progressively during the follicular phase. Enhanced pituitary responsiveness to LH-RH occurred at mid-cycle for both LH and FSH and maximum LH responses occurred during the \"LH Surge\" and early luteal phase. LH responses during the mid and late luteal phases were similar to late follicular phase responses. There were no significant differences between FSH responses during the early follicular, late follicular, mid-luteal and late luteal phases. Maximum pituitary responsiveness appears to occur in a gonadal steroid milieu of high oestrogen levels in association with rising but low progesterone levels. Progesterone or a crucial oestrogen: progesterone ratio may in fact potentiate pituitary release of LH during the early stages of corpus luteum formation. Pituitary responsiveness to LH-RH correlates positively with basal LH and oestrogen levels during the menstrual cycle and with the oestrogen:progesterone ratio during the luteal phase.", "contents": "The sequence of pituitary responses to synthetic luteinizing hormone releasing hormone (LH-RH) throughout the normal menstrual cycle. Thirty-one ovulatory women between 20 and 33 years of age were given 150 mug of synthetic LH-RH during different phases of the menstrual cycle. Five patients were studied during the early follicular phase (days 4-7); 10 patients during the late follicular phase (days 9-12); 6 patients during the \"LH Surge\"; 5 patients during the early luteal phase (days 14-16); 3 patients during mid-luteal phase (days 17-21); and 2 patients during late luteal phase (days 22-27). Oestrogen, progesterone, FSH and LH levels were determined from 30 min prior to LH-RH administration to 90 min thereafter in all cases. LH response to LH-RH increased progressively during the follicular phase. Enhanced pituitary responsiveness to LH-RH occurred at mid-cycle for both LH and FSH and maximum LH responses occurred during the \"LH Surge\" and early luteal phase. LH responses during the mid and late luteal phases were similar to late follicular phase responses. There were no significant differences between FSH responses during the early follicular, late follicular, mid-luteal and late luteal phases. Maximum pituitary responsiveness appears to occur in a gonadal steroid milieu of high oestrogen levels in association with rising but low progesterone levels. Progesterone or a crucial oestrogen: progesterone ratio may in fact potentiate pituitary release of LH during the early stages of corpus luteum formation. Pituitary responsiveness to LH-RH correlates positively with basal LH and oestrogen levels during the menstrual cycle and with the oestrogen:progesterone ratio during the luteal phase."}
{"id": "PMID:1098350", "title": "Testosterone undecanoate: a new orally active androgen.", "content": "Oral testosterone undecanoate (TU) in arachis oil has been evaluated with a view to its possible use as a means of androgen replacement therapy. A single 100 mg dose was found to elevate plasma androgen levels and urinary 17-ketosteroid excretion in 6 normal men. Ninety mg/day and 60 mg/day doses taken by a hypogonadal man resulted in sustained levels of androgen which appeared physiological when measured by radioimmunoassay without chromatography. However, upon separation of the steroids by chromatography it was found that much of the androgen present was in fact dihydrotestosterone not testosterone. Both TU and dihydrotestosterone undecanoate were detected in plasma by gas chromatography and it is suggested that the ester is absorbed as such from the intestine and the unesterified steroid subsequently released by hydrolysis. The convenience of oral administration, the resulting prolonged elevated plasma androgen levels and the probable lack of deleterious effects on the liver may render oral TU of value where androgen replacement therapy is indicated.", "contents": "Testosterone undecanoate: a new orally active androgen. Oral testosterone undecanoate (TU) in arachis oil has been evaluated with a view to its possible use as a means of androgen replacement therapy. A single 100 mg dose was found to elevate plasma androgen levels and urinary 17-ketosteroid excretion in 6 normal men. Ninety mg/day and 60 mg/day doses taken by a hypogonadal man resulted in sustained levels of androgen which appeared physiological when measured by radioimmunoassay without chromatography. However, upon separation of the steroids by chromatography it was found that much of the androgen present was in fact dihydrotestosterone not testosterone. Both TU and dihydrotestosterone undecanoate were detected in plasma by gas chromatography and it is suggested that the ester is absorbed as such from the intestine and the unesterified steroid subsequently released by hydrolysis. The convenience of oral administration, the resulting prolonged elevated plasma androgen levels and the probable lack of deleterious effects on the liver may render oral TU of value where androgen replacement therapy is indicated."}
{"id": "PMID:1098352", "title": "Mast cell sensitizing antibodies in experimental candidiasis.", "content": "Inoculation into foot-pads of guinea-pigs of a living suspension of Candida albicans together with Complete Freund's Adjuvant, provoked a hypersensitive state, characterized by: 1) positive skin tests; 2) direct degranulation of peritoneal mast cells when exposed in vitro to the antigen; and 3) homologous \"mast cell sensitizing antibodies\" detected by the \"indirect mast cell degranulation test\". The mast cell sensitizing antibodies were heat labile, and their activity could not be restored by complement.", "contents": "Mast cell sensitizing antibodies in experimental candidiasis. Inoculation into foot-pads of guinea-pigs of a living suspension of Candida albicans together with Complete Freund's Adjuvant, provoked a hypersensitive state, characterized by: 1) positive skin tests; 2) direct degranulation of peritoneal mast cells when exposed in vitro to the antigen; and 3) homologous \"mast cell sensitizing antibodies\" detected by the \"indirect mast cell degranulation test\". The mast cell sensitizing antibodies were heat labile, and their activity could not be restored by complement."}
{"id": "PMID:1098353", "title": "Treatment of children with perennial rhinitis with brompheniraminemaleate and phenylpropanolaminehydrochloride (lunerin mite)", "content": "The effect of a combined pediatric preparation of brompheniramine maleate and phenylpropanolaminehydrochloride (Lunerin mite) was studied in 17 children (aged 4-14 years) with allergic rhinitis. A double-blind cross-over technique was used, and a score system was used for evaluation of symptoms and signs. The results show that the drug had a good effect with statistical differences on the 1 per cent level between the active preparation and the placebo. The effect was less pronounced in children aged more than 10 years, for whom a higher dose of the drug is recommended. The frequency of side-effects was negligible.", "contents": "Treatment of children with perennial rhinitis with brompheniraminemaleate and phenylpropanolaminehydrochloride (lunerin mite). The effect of a combined pediatric preparation of brompheniramine maleate and phenylpropanolaminehydrochloride (Lunerin mite) was studied in 17 children (aged 4-14 years) with allergic rhinitis. A double-blind cross-over technique was used, and a score system was used for evaluation of symptoms and signs. The results show that the drug had a good effect with statistical differences on the 1 per cent level between the active preparation and the placebo. The effect was less pronounced in children aged more than 10 years, for whom a higher dose of the drug is recommended. The frequency of side-effects was negligible."}
{"id": "PMID:1098354", "title": "Mast cell sensitizing antibody (MCSAb) response in experimental candidiasis: chromatographic studies.", "content": "A single dose of the yeast-like fungus Candida albicans (CA), mixed with Complete Freund's Adjuvant (CFA), and inoculated into guinea-pig foot-pads, provoked the productions of homologous mast cell sensitizing antibodies (MCSAb) present in the psiI-globulin fraction only, and of agglutinins localized in the same fraction. Heterologous MCSAb and skin-sensitizing antibodies detectable by passive cutaneous anaphylaxis (PCA) were constantly absent. These facts are in contradiction to results of previous experiments performed with other antigens; which after similar inoculation elicited production of heterologous MCSAb attaching themselves on rat mast cells (MC) and present in the psi2 fast moving globulin, homologous MCSAb localized in the psiI-globulin and in the psi2 fast moving globulin, and PCA antibodies. The immunogenicity of CA is discussed.", "contents": "Mast cell sensitizing antibody (MCSAb) response in experimental candidiasis: chromatographic studies. A single dose of the yeast-like fungus Candida albicans (CA), mixed with Complete Freund's Adjuvant (CFA), and inoculated into guinea-pig foot-pads, provoked the productions of homologous mast cell sensitizing antibodies (MCSAb) present in the psiI-globulin fraction only, and of agglutinins localized in the same fraction. Heterologous MCSAb and skin-sensitizing antibodies detectable by passive cutaneous anaphylaxis (PCA) were constantly absent. These facts are in contradiction to results of previous experiments performed with other antigens; which after similar inoculation elicited production of heterologous MCSAb attaching themselves on rat mast cells (MC) and present in the psi2 fast moving globulin, homologous MCSAb localized in the psiI-globulin and in the psi2 fast moving globulin, and PCA antibodies. The immunogenicity of CA is discussed."}
{"id": "PMID:1098356", "title": "Effect of cobalt salts on the glycemia and islet histology of Channa punctatus (Bloch).", "content": "Channa punctatus responds to the intramuscular administration of cobalt chloride and cobalt nitrate by exhibiting an increase in the blood glucose level. The hyperglycemia persists till 120 h, after which the fish become moribund and ultimately die. Cobalt salts evoke degranulation and vacuolization of the alpha-cells in the initial stage but later the beta-cells are also found to be severely damaged. Cobalt chloride has been found to be more toxic than the cobalt nitrate.", "contents": "Effect of cobalt salts on the glycemia and islet histology of Channa punctatus (Bloch). Channa punctatus responds to the intramuscular administration of cobalt chloride and cobalt nitrate by exhibiting an increase in the blood glucose level. The hyperglycemia persists till 120 h, after which the fish become moribund and ultimately die. Cobalt salts evoke degranulation and vacuolization of the alpha-cells in the initial stage but later the beta-cells are also found to be severely damaged. Cobalt chloride has been found to be more toxic than the cobalt nitrate."}
{"id": "PMID:1098357", "title": "Scintillation camera studies of isotope flow in arteriovenous malformations.", "content": "A significant proportion of arteriovenous malformations are still undetected on conventional brain scans. Dynamic scintillation camera studies have shown a pronounced and rapid isotope accumulation in these structures. By means of data processed flow studies, one can distinctly select the inflow phase etc., display TV pictures in optimal phase, and make quantitative comparisons. Thereby, one can obtain a very probable diagnosis, even when the AVM is small or has the characteristics of a fistula which is not seen on routine scans. As the method is simple and not time consuming, it is well suited for clinical screening.", "contents": "Scintillation camera studies of isotope flow in arteriovenous malformations. A significant proportion of arteriovenous malformations are still undetected on conventional brain scans. Dynamic scintillation camera studies have shown a pronounced and rapid isotope accumulation in these structures. By means of data processed flow studies, one can distinctly select the inflow phase etc., display TV pictures in optimal phase, and make quantitative comparisons. Thereby, one can obtain a very probable diagnosis, even when the AVM is small or has the characteristics of a fistula which is not seen on routine scans. As the method is simple and not time consuming, it is well suited for clinical screening."}
{"id": "PMID:1098351", "title": "[4-year-observation of results of VM 26 treatment of pathologic syndromes from the lymphoma malignum group].", "content": "The  authors used the preparation VM 26 in 66 patients with various pathological syndromes belonging to the group of malignant lymphoma. The patients were divided into three groups. Group A received only VM 26, group B VM 26 and ultracorten, group C VM 26, cyclophosphamide, methylhydrazine and glycocorticosteroids. In all observed groups a therapeutic activity of VM 26 was demonstrated. The best therapeutic results were obtained in group C, in group B they were slightly worse.", "contents": "[4-year-observation of results of VM 26 treatment of pathologic syndromes from the lymphoma malignum group]. The  authors used the preparation VM 26 in 66 patients with various pathological syndromes belonging to the group of malignant lymphoma. The patients were divided into three groups. Group A received only VM 26, group B VM 26 and ultracorten, group C VM 26, cyclophosphamide, methylhydrazine and glycocorticosteroids. In all observed groups a therapeutic activity of VM 26 was demonstrated. The best therapeutic results were obtained in group C, in group B they were slightly worse."}
{"id": "PMID:1098358", "title": "Subjective symptoms in epileptic patients on anticonvulsant drugs. A controlled therapeutic trial on the effect of vitamin d.", "content": "The possibility (based on the recognised existence of anticonvulsant osteomalacia), of an osteomalacic origin of a number of subjective symptoms in epileptics (back pain, tiredness, sleepiness, irritability, and giddiness) was tested during a controlled therapeutic trial in 226 outpatients. There was no correlation between subjective symptoms and objective pathological indices of osteomalacia, and group treated with vitamin D (2000 international units daily for 3 months) showed no amelioration of subjective symptoms above that seen in the placebo group. The findings do not support the view that all epileptic patients on anticonvulsant therapy should be treated prophylactically with vitamin D.", "contents": "Subjective symptoms in epileptic patients on anticonvulsant drugs. A controlled therapeutic trial on the effect of vitamin d. The possibility (based on the recognised existence of anticonvulsant osteomalacia), of an osteomalacic origin of a number of subjective symptoms in epileptics (back pain, tiredness, sleepiness, irritability, and giddiness) was tested during a controlled therapeutic trial in 226 outpatients. There was no correlation between subjective symptoms and objective pathological indices of osteomalacia, and group treated with vitamin D (2000 international units daily for 3 months) showed no amelioration of subjective symptoms above that seen in the placebo group. The findings do not support the view that all epileptic patients on anticonvulsant therapy should be treated prophylactically with vitamin D."}
{"id": "PMID:1098359", "title": "Piribedil (ET 495) in the treatment of Parkinson's disease combined with amantadine or levodopa.", "content": "The further therapeutic benefit of piribedil when combined with amantadine or Levodopa was studied by a double-blind, cross-over trial in 15 patients with Parkinson's disease. A significant improvement at the 5 per cent level for akinesia, gait, speech disorder and facial expression occurred when piribedil was added to Levodopa; and a more highly significant improvement at the 1 per cent level for akinesia, facial expression and finger dexterity occurred with piribedil and amantadine. No significant improvement occurred for special timed tests. Improvement was associated with side effects in both groups of patients. Side effects occurred with both placebo and active piribedil. Only nausea during piribedil and Levodopa treatment reached statistical significance when compared with the placebo. Piribedil did not give rise to any haematological or biochemical complications. Our findings suggest that piribedil is of further therapeutic benefit when added to amantadine or Levodopa. It was suggested that the improvement which occurred together with amantadine could be due to the combined action of both drugs on dopamine receptors.", "contents": "Piribedil (ET 495) in the treatment of Parkinson's disease combined with amantadine or levodopa. The further therapeutic benefit of piribedil when combined with amantadine or Levodopa was studied by a double-blind, cross-over trial in 15 patients with Parkinson's disease. A significant improvement at the 5 per cent level for akinesia, gait, speech disorder and facial expression occurred when piribedil was added to Levodopa; and a more highly significant improvement at the 1 per cent level for akinesia, facial expression and finger dexterity occurred with piribedil and amantadine. No significant improvement occurred for special timed tests. Improvement was associated with side effects in both groups of patients. Side effects occurred with both placebo and active piribedil. Only nausea during piribedil and Levodopa treatment reached statistical significance when compared with the placebo. Piribedil did not give rise to any haematological or biochemical complications. Our findings suggest that piribedil is of further therapeutic benefit when added to amantadine or Levodopa. It was suggested that the improvement which occurred together with amantadine could be due to the combined action of both drugs on dopamine receptors."}
{"id": "PMID:1098360", "title": "Intranuclear inclusions in a case of Creutzfeldt-Jakob disease: an ultrastructural study.", "content": "Two types of intranuclear inclusions were described in a brain biopsy from a patient with Creutzfeldt-Jakob disease. The first type of intranculear inclusion was papova virus-like and was observed within 20% of the nuclei of all astrocytes and neurons examined. The particles measured 32 nm in diameter and consisted of a dense core surrounded by a multi-layered shell. The second type of intranuclear inculsion was a granulo-fibrillar nuclear body found within the nuclei of astrocytes and neurons. The possible significance of these inculsions to the etiology of Creutzfeldt-Jakob disease is discussed.", "contents": "Intranuclear inclusions in a case of Creutzfeldt-Jakob disease: an ultrastructural study. Two types of intranuclear inclusions were described in a brain biopsy from a patient with Creutzfeldt-Jakob disease. The first type of intranculear inclusion was papova virus-like and was observed within 20% of the nuclei of all astrocytes and neurons examined. The particles measured 32 nm in diameter and consisted of a dense core surrounded by a multi-layered shell. The second type of intranuclear inculsion was a granulo-fibrillar nuclear body found within the nuclei of astrocytes and neurons. The possible significance of these inculsions to the etiology of Creutzfeldt-Jakob disease is discussed."}
{"id": "PMID:1098361", "title": "Morphology and classification of malignant lymphomas and so-called reticuloses.", "content": "There is general agreement as to the definition and subclassification of Hodgkin lymphomas, which is acceptable for practical reasons. However, the nature of the different types of Hodgkin lymphomas needs further consideration. On the other hand, today we are as far from agreement on non-Hodgkin lymphomas as ever before. Earlier European and American classifications have now to be reconsidered in the light of modern concepts of experimental immunology. It has become necessary to apply immunochemical and immunomorphological methods in addition to histological, histochemical, cytological, and ultrastructural techniques. Only such a complex approach has been able to provide new insight into the functional properties of the tumor cells. The result was a new lymphoma classification, which conforms to LUKES' concept in many respects. It was fundamentally accepted by a group of European lymphoma experts and has been condensed and modified in order to be applicable by as many lymphoma centers as possible. It has received the name \"Kiel Classification\" 1974. The main principles of the classification are as follows: 1. A distinction is made between malignant lymphomas of low-grade and those of high-grade malignancy; \"-cytic\", \"-blastic\" m.l. 2. The terms \"sarcoma\" and \"leukemia\" are avoided. All types of malignant lymphoma can be leukemic, however with differnt frequencies. All B-cell lymphomas can also show a monoclonal immunoglobulin increase (\"paraproteinemia\") in the blood, mostly of IgM. So-called macroglobulinemia of Waldenstr\u00f6m is therefore not itself an etity, but instead a clinical syndrome. It has been shown that so-called reticulosarcoma is derived from immunoblasts, not from reticulum cells or histiocytes. The group of reticuloses also needs reconsideration. The term \"reticulosis\" is cytologically incorrect in most cases. Otherwise it refers to a group of diseases which we do not yet understand.", "contents": "Morphology and classification of malignant lymphomas and so-called reticuloses. There is general agreement as to the definition and subclassification of Hodgkin lymphomas, which is acceptable for practical reasons. However, the nature of the different types of Hodgkin lymphomas needs further consideration. On the other hand, today we are as far from agreement on non-Hodgkin lymphomas as ever before. Earlier European and American classifications have now to be reconsidered in the light of modern concepts of experimental immunology. It has become necessary to apply immunochemical and immunomorphological methods in addition to histological, histochemical, cytological, and ultrastructural techniques. Only such a complex approach has been able to provide new insight into the functional properties of the tumor cells. The result was a new lymphoma classification, which conforms to LUKES' concept in many respects. It was fundamentally accepted by a group of European lymphoma experts and has been condensed and modified in order to be applicable by as many lymphoma centers as possible. It has received the name \"Kiel Classification\" 1974. The main principles of the classification are as follows: 1. A distinction is made between malignant lymphomas of low-grade and those of high-grade malignancy; \"-cytic\", \"-blastic\" m.l. 2. The terms \"sarcoma\" and \"leukemia\" are avoided. All types of malignant lymphoma can be leukemic, however with differnt frequencies. All B-cell lymphomas can also show a monoclonal immunoglobulin increase (\"paraproteinemia\") in the blood, mostly of IgM. So-called macroglobulinemia of Waldenstr\u00f6m is therefore not itself an etity, but instead a clinical syndrome. It has been shown that so-called reticulosarcoma is derived from immunoblasts, not from reticulum cells or histiocytes. The group of reticuloses also needs reconsideration. The term \"reticulosis\" is cytologically incorrect in most cases. Otherwise it refers to a group of diseases which we do not yet understand."}
{"id": "PMID:1098362", "title": "Primary and borderline brain lymphosarcoma: a neuropathological review of nine cases.", "content": "Brain lymphosarcoma may be divided into the circumscribed forms, surrounded by marked oedema with fibrin, and the diffuse or infiltrating forms. Cytology of the former is more uniformly lymphocytic, its clinical course is more rapidly fatal. Anatomically primary diffuse lymphosarcoma of the brain may be secondary to 'cured' systemic lymphoma.", "contents": "Primary and borderline brain lymphosarcoma: a neuropathological review of nine cases. Brain lymphosarcoma may be divided into the circumscribed forms, surrounded by marked oedema with fibrin, and the diffuse or infiltrating forms. Cytology of the former is more uniformly lymphocytic, its clinical course is more rapidly fatal. Anatomically primary diffuse lymphosarcoma of the brain may be secondary to 'cured' systemic lymphoma."}
{"id": "PMID:1098363", "title": "The classification of microgliomatosis with particular reference to diffuse microgliomatosis.", "content": "In 13 of 14 autopsied cases of microgliomatosis, there was macroscopic evidence of tumour in the brain. In 11 of these 13 cases, microscopical examination disclosed many other small foci of microglimatosis. In the brain which appeared normal macroscopically, there was only diffuse microgliomatosis. This was histologically indistinguishable from that found in the cases with distinct masses of tumour.", "contents": "The classification of microgliomatosis with particular reference to diffuse microgliomatosis. In 13 of 14 autopsied cases of microgliomatosis, there was macroscopic evidence of tumour in the brain. In 11 of these 13 cases, microscopical examination disclosed many other small foci of microglimatosis. In the brain which appeared normal macroscopically, there was only diffuse microgliomatosis. This was histologically indistinguishable from that found in the cases with distinct masses of tumour."}
{"id": "PMID:1098364", "title": "Patterns of proliferation in cerebral lymphoreticular tumours.", "content": "A study of eighteen cases of primary lymphoreticular tumours in the brain is described. In four of these there were extraneural lesions and in one macroglobulinaema. The use of whole brain sections embedded in celloidin, and of metallic impregnation methods, revealed certain constant patterns of proliferation. The tumours were diffuse and multicentric; the leptomeninges and perivascular spaces especially in the subependymal regions were frequently involved. Mature microglia were active both in infiltrated and in apparently tumour-free regions.", "contents": "Patterns of proliferation in cerebral lymphoreticular tumours. A study of eighteen cases of primary lymphoreticular tumours in the brain is described. In four of these there were extraneural lesions and in one macroglobulinaema. The use of whole brain sections embedded in celloidin, and of metallic impregnation methods, revealed certain constant patterns of proliferation. The tumours were diffuse and multicentric; the leptomeninges and perivascular spaces especially in the subependymal regions were frequently involved. Mature microglia were active both in infiltrated and in apparently tumour-free regions."}
{"id": "PMID:1098365", "title": "\"Malignant lymphoma\" of the brain following renal transplantation.", "content": "A patient is described who was thought to have developed a malignant lymphoma of the brain after renal transplantation. The correct diagnosis was toxoplasmosis.", "contents": "\"Malignant lymphoma\" of the brain following renal transplantation. A patient is described who was thought to have developed a malignant lymphoma of the brain after renal transplantation. The correct diagnosis was toxoplasmosis."}
{"id": "PMID:1098366", "title": "Human and experimental reticulum cell sarcoma (microglioma) of the nervous system.", "content": "The histology and ultrastructure of primary central nervous system and metastatic (epidural) reticulum cell sarcoma is described. The tumors showed varying numbers of argyrophilic microglial cells which in all cases constituted a minority of all tumor cells. A primitive, undifferentiated cell, common to all tumors, was a small cell with a large dense nucleus and a narrow rim of poorly structured cytoplasm. These cells frequently showed small fatty inclusions and were tentatively identified as primitive multipotential cells from which all other tumor cells develop. It is concluded that reticulum cell sarcoma of the nervous system (primary or metastatic) and microglioma or microgliomatosis are one and the same tumor.", "contents": "Human and experimental reticulum cell sarcoma (microglioma) of the nervous system. The histology and ultrastructure of primary central nervous system and metastatic (epidural) reticulum cell sarcoma is described. The tumors showed varying numbers of argyrophilic microglial cells which in all cases constituted a minority of all tumor cells. A primitive, undifferentiated cell, common to all tumors, was a small cell with a large dense nucleus and a narrow rim of poorly structured cytoplasm. These cells frequently showed small fatty inclusions and were tentatively identified as primitive multipotential cells from which all other tumor cells develop. It is concluded that reticulum cell sarcoma of the nervous system (primary or metastatic) and microglioma or microgliomatosis are one and the same tumor."}
{"id": "PMID:1098367", "title": "Fine structure of primary reticulum cell sacroma of the brain.", "content": "An electron microscopic study was made on two cases of reticulum cell sarcoma (microglioma) of the brain. In both, the tumour was composed of several type of cells; cells with an undifferentiated appearance and cells with ultrastructural features of the reticulum cell and histiocyte. There seemed to be no significant difference in the fine structure between the tumour cells in reticulum cell sarcoma of the brain and extracerebral forms. The ultrastructures of a lymphosarcoma of the spinal epidural space was also examined and resembles that of such tumours occurring elsewhere in the body.", "contents": "Fine structure of primary reticulum cell sacroma of the brain. An electron microscopic study was made on two cases of reticulum cell sarcoma (microglioma) of the brain. In both, the tumour was composed of several type of cells; cells with an undifferentiated appearance and cells with ultrastructural features of the reticulum cell and histiocyte. There seemed to be no significant difference in the fine structure between the tumour cells in reticulum cell sarcoma of the brain and extracerebral forms. The ultrastructures of a lymphosarcoma of the spinal epidural space was also examined and resembles that of such tumours occurring elsewhere in the body."}
{"id": "PMID:1098368", "title": "Primary cerebral reticulosis and plasma cell diffenentiation.", "content": "Cerebral proliferative and tumour forming reticulosis are primary pathologic processes of the brain originating in the perivascular adventitial cell. In these conditions this cell can transform into a histiocyte or microglial cell with characters of a macrophage. A reactive cell with endoplasmic reticulum, basement membrane and fibril forming properties, is also present (fibril forming reticular cell). The malignant tumours of the perivascular spaces (cerebral reticulosarcoma) are characterized by the presence of the dedifferentiated cells originating from the intraadventitial cell. Under both proliferative and tumour forming conditions this cell may transform into a cell rich in endoplasmic reticulum, sometimes charged with Russell bodies and corresponds morphologically to the protein-forming plasma cell. The properties of the intracerebral periadventitial cells are identical with those of the reticulo-histiocytic system described by ASCHOFF. As its structure and the elements into which it differentiats sharply differ from those of the cells belonging to the lymphocyte series the term malignant lymphoma of the brain given to these processes does not correspond to the fundamental characters of these pathological process.", "contents": "Primary cerebral reticulosis and plasma cell diffenentiation. Cerebral proliferative and tumour forming reticulosis are primary pathologic processes of the brain originating in the perivascular adventitial cell. In these conditions this cell can transform into a histiocyte or microglial cell with characters of a macrophage. A reactive cell with endoplasmic reticulum, basement membrane and fibril forming properties, is also present (fibril forming reticular cell). The malignant tumours of the perivascular spaces (cerebral reticulosarcoma) are characterized by the presence of the dedifferentiated cells originating from the intraadventitial cell. Under both proliferative and tumour forming conditions this cell may transform into a cell rich in endoplasmic reticulum, sometimes charged with Russell bodies and corresponds morphologically to the protein-forming plasma cell. The properties of the intracerebral periadventitial cells are identical with those of the reticulo-histiocytic system described by ASCHOFF. As its structure and the elements into which it differentiats sharply differ from those of the cells belonging to the lymphocyte series the term malignant lymphoma of the brain given to these processes does not correspond to the fundamental characters of these pathological process."}
{"id": "PMID:1098369", "title": "Ultrastructural features of human lymphomas.", "content": "The diagnostic value of some ultrastructural details in a series of 73 lymphomas, 7 thymomas, 6 cases of Waldenstr\u00f6m's disease, and 5 myelomas has been critically reviewed. The light microscopical diagnoses of \"reticulum cell sarcoma\" seems now inadvisable, since thhe majority of these cases, when examined by electron microscopy, were found to be \"blast cell sarcomas\", probably lymphoid in nature. Clear-cut relationships between cell ultrastructure and immunfluorescence data about surface Ig in lymphoid cell populations have not been ascertained.", "contents": "Ultrastructural features of human lymphomas. The diagnostic value of some ultrastructural details in a series of 73 lymphomas, 7 thymomas, 6 cases of Waldenstr\u00f6m's disease, and 5 myelomas has been critically reviewed. The light microscopical diagnoses of \"reticulum cell sarcoma\" seems now inadvisable, since thhe majority of these cases, when examined by electron microscopy, were found to be \"blast cell sarcomas\", probably lymphoid in nature. Clear-cut relationships between cell ultrastructure and immunfluorescence data about surface Ig in lymphoid cell populations have not been ascertained."}
{"id": "PMID:1098370", "title": "Certain notable clinical attributes of the histiocytic sarcomas of the central nervous system.", "content": "By the term reticulum cell sarcoma we denote any tumor composed predominantly of undifferentiated cells, some of which have the qualities of histiocytes. The origin of such CNS tumors may be traced to circulating monocytes, perithelial ro meningeal histiocytes or microgliocytes. The ubiquity of cells of the monocyte-histiocyte series allows six possibilities of CNS involvement: a) primary in the brain: b) secondarily involve the brain or spinal cord by extending from a cranial bone or vertebra to the epidural space c) rarely to involve intraneuronal tissues (lymph nodes, bone, viscera) and then later to localize to the brain substance d) to spread from brain outside the nervous system e) to evoke any one or several of the paraneoplastic diseases (polymyositis, polyneuritis, cerebellar degeneration, f) to permit widespread infections of the nervous system such as multifocal leucoencephalitis. Clinical attributes to be emphasized are the relative rarity of hematogenous metastases (2 of 121 cases), the relatively high incidence of such tumors in immunologically suppressed individuals (12 of 5000 cases), the frequency of primary tumors of CNS (23 of 144 cases), the high incidence of epidural and dural involvement from osseous lesions (13 of 121 cases); the rapid evolution of clinical phenomena; the rarity of paraneoplastic syndromes; the occasional spontaneous and frequent therapeutic regression upon x-radiation. The common invasion of pia and ependyma by the tumor cells and their natural tendency to phagocytosis opens unrealized possibilities of clinical diagnosis by cytological examination and culture of CSF. Early diagnosis by these methods permits avoidance of surgery and the use of radiation and possibly chemotherapy, which may be rewarded by symptomatic regression and potential cure.", "contents": "Certain notable clinical attributes of the histiocytic sarcomas of the central nervous system. By the term reticulum cell sarcoma we denote any tumor composed predominantly of undifferentiated cells, some of which have the qualities of histiocytes. The origin of such CNS tumors may be traced to circulating monocytes, perithelial ro meningeal histiocytes or microgliocytes. The ubiquity of cells of the monocyte-histiocyte series allows six possibilities of CNS involvement: a) primary in the brain: b) secondarily involve the brain or spinal cord by extending from a cranial bone or vertebra to the epidural space c) rarely to involve intraneuronal tissues (lymph nodes, bone, viscera) and then later to localize to the brain substance d) to spread from brain outside the nervous system e) to evoke any one or several of the paraneoplastic diseases (polymyositis, polyneuritis, cerebellar degeneration, f) to permit widespread infections of the nervous system such as multifocal leucoencephalitis. Clinical attributes to be emphasized are the relative rarity of hematogenous metastases (2 of 121 cases), the relatively high incidence of such tumors in immunologically suppressed individuals (12 of 5000 cases), the frequency of primary tumors of CNS (23 of 144 cases), the high incidence of epidural and dural involvement from osseous lesions (13 of 121 cases); the rapid evolution of clinical phenomena; the rarity of paraneoplastic syndromes; the occasional spontaneous and frequent therapeutic regression upon x-radiation. The common invasion of pia and ependyma by the tumor cells and their natural tendency to phagocytosis opens unrealized possibilities of clinical diagnosis by cytological examination and culture of CSF. Early diagnosis by these methods permits avoidance of surgery and the use of radiation and possibly chemotherapy, which may be rewarded by symptomatic regression and potential cure."}
{"id": "PMID:1098371", "title": "Differential diagnostic aspects in malignant lymphomas involving the central nervous system.", "content": "Neoplastic cells were found in the cerebrospinal fluid (CSF) cytograms in 57% of patients with lymphocytic leukemia and reticulum cell sarcoma. Tumor cells may be observed in CSF specimens showing normal cell count and total proteins. Quantitative and/or qualitative changes in CSF proteins in absence of corresponding abnormalities in serum may indicate a malignant lymphorproliferative process involving the central nervous system even in absence of neoplastic cells.", "contents": "Differential diagnostic aspects in malignant lymphomas involving the central nervous system. Neoplastic cells were found in the cerebrospinal fluid (CSF) cytograms in 57% of patients with lymphocytic leukemia and reticulum cell sarcoma. Tumor cells may be observed in CSF specimens showing normal cell count and total proteins. Quantitative and/or qualitative changes in CSF proteins in absence of corresponding abnormalities in serum may indicate a malignant lymphorproliferative process involving the central nervous system even in absence of neoplastic cells."}
{"id": "PMID:1098372", "title": "Light- and electron microscopic studies in hemoblastosis with CNS-disorder.", "content": "Some cases of hemoblastosis with CNS-involvement were investigated by methods of light microscopy, cytochemistry and electronmicroscopy. The unique cell picture, as observed by light microscopy could not be proven by electronmicroscopic results. This fact was supported by comparative investigations of inflammation processes. Typical structures are described and discusses.", "contents": "Light- and electron microscopic studies in hemoblastosis with CNS-disorder. Some cases of hemoblastosis with CNS-involvement were investigated by methods of light microscopy, cytochemistry and electronmicroscopy. The unique cell picture, as observed by light microscopy could not be proven by electronmicroscopic results. This fact was supported by comparative investigations of inflammation processes. Typical structures are described and discusses."}
{"id": "PMID:1098373", "title": "Clinical and pathological studies in a case of reticulum cell sarcoma.", "content": "A case of reticulum cell sarcoma associated with involvement of the central nervous system was presented. This seems to be an atypical case of reticulum cell sarcoma for the following reasons. 1. Hepatosplenomegaly and enlarged lymph nodes were not observed through the entire clinical course. The main abnormalities were psycho-neurological. 2. These cells did not form a tumour mass, but infiltrated diffucely into the central nervous system and extraneural sites, and the primary focus could not be determined. Four cases of reticulum cell sarcoma in Japan, in which the initial symptoms were of neurological involvement, are reviewed.", "contents": "Clinical and pathological studies in a case of reticulum cell sarcoma. A case of reticulum cell sarcoma associated with involvement of the central nervous system was presented. This seems to be an atypical case of reticulum cell sarcoma for the following reasons. 1. Hepatosplenomegaly and enlarged lymph nodes were not observed through the entire clinical course. The main abnormalities were psycho-neurological. 2. These cells did not form a tumour mass, but infiltrated diffucely into the central nervous system and extraneural sites, and the primary focus could not be determined. Four cases of reticulum cell sarcoma in Japan, in which the initial symptoms were of neurological involvement, are reviewed."}
{"id": "PMID:1098374", "title": "Diffuse reticulosis with leukomalacia.", "content": "A malignant lymphoproliferative disease with acute clinical course and selective clinical CNS involvement is described. The metastatic lesions in the brain are compared with a primary microglioma of the brain.", "contents": "Diffuse reticulosis with leukomalacia. A malignant lymphoproliferative disease with acute clinical course and selective clinical CNS involvement is described. The metastatic lesions in the brain are compared with a primary microglioma of the brain."}
{"id": "PMID:1098375", "title": "Differentiation of lymphoid cells of the B cell series according to membrane and cytoplasmic determinants.", "content": "Previous investigations have shown that a large proportion of normal human lymphocytes bearing membrane-bound IgM have also independently moving IgD molecules on their surface. Since it is known that in the majority of chronic lymphatic leukaemias (CLL) high numbers of lymphocytes with easily detectable membrane IgM can be found, we investigated CLL cells for the presence of membrane-bound IgD. In all 18 cases studied, high proportions of cells bearing surface IgD and IgM could be found. In none of the 18 cases crystalline Ig inclusions could be detected.", "contents": "Differentiation of lymphoid cells of the B cell series according to membrane and cytoplasmic determinants. Previous investigations have shown that a large proportion of normal human lymphocytes bearing membrane-bound IgM have also independently moving IgD molecules on their surface. Since it is known that in the majority of chronic lymphatic leukaemias (CLL) high numbers of lymphocytes with easily detectable membrane IgM can be found, we investigated CLL cells for the presence of membrane-bound IgD. In all 18 cases studied, high proportions of cells bearing surface IgD and IgM could be found. In none of the 18 cases crystalline Ig inclusions could be detected."}
{"id": "PMID:1098376", "title": "Malignant lymphomas of the nervous system.", "content": "In a series of some 7,000 patients with tumors of the central nervous system, 208 patients (about 3%) had some form of a malignant lymphoma. Slightly less than half of these tumors were primary in the brain; the remainder had cranial involvement as part of a generalized process. The tumors consisted of Hodgkin's disease, lymphosarcomas, reticulosarcomas and plasmacytomas. The brain was involved in one of two ways: either as localized tumor masses resembling certain gliomas, or as diffusely invasive neoplasms resembling exudative cellular inflammatory processes. They had a peculiar predilection for the septum pellucidum but occurred also in the cerebral lobes, basal ganglia, brain stem and cerebellum. They all produced a fibrillary stroma of reticulin fibers and they spread along the perivascular spaces, in the cerebrospinal subarachnoid space, or intraventricularly on and beneath the ependymal lining. One type of lymphoma often fused into another - thus a single tumor often consisted of Hodgkin's sarcoma, lymphosarcoma and reticulosarcoma. In an addition series of 57 cases of spinal cord involvement by malignant lymphomas, there were no instances of a primary tumor; all patients had either primary lymphomas of the brain with secondary spread to the spinal subarchnoid space, or had spinal cord compression as a result of tumor in the vertebrae, the spinal epidural space, or the spinal dura. Hence the spinal cord involvement was a secondary manifestation of a lymphoma elsewhere. Peripheral nerve involvement by lymphomas resulted in destruction of myelin sheaths and axons by tumor cell infiltration and the neuropathy was always part of a generalized lymphomatosis.", "contents": "Malignant lymphomas of the nervous system. In a series of some 7,000 patients with tumors of the central nervous system, 208 patients (about 3%) had some form of a malignant lymphoma. Slightly less than half of these tumors were primary in the brain; the remainder had cranial involvement as part of a generalized process. The tumors consisted of Hodgkin's disease, lymphosarcomas, reticulosarcomas and plasmacytomas. The brain was involved in one of two ways: either as localized tumor masses resembling certain gliomas, or as diffusely invasive neoplasms resembling exudative cellular inflammatory processes. They had a peculiar predilection for the septum pellucidum but occurred also in the cerebral lobes, basal ganglia, brain stem and cerebellum. They all produced a fibrillary stroma of reticulin fibers and they spread along the perivascular spaces, in the cerebrospinal subarachnoid space, or intraventricularly on and beneath the ependymal lining. One type of lymphoma often fused into another - thus a single tumor often consisted of Hodgkin's sarcoma, lymphosarcoma and reticulosarcoma. In an addition series of 57 cases of spinal cord involvement by malignant lymphomas, there were no instances of a primary tumor; all patients had either primary lymphomas of the brain with secondary spread to the spinal subarchnoid space, or had spinal cord compression as a result of tumor in the vertebrae, the spinal epidural space, or the spinal dura. Hence the spinal cord involvement was a secondary manifestation of a lymphoma elsewhere. Peripheral nerve involvement by lymphomas resulted in destruction of myelin sheaths and axons by tumor cell infiltration and the neuropathy was always part of a generalized lymphomatosis."}
{"id": "PMID:1098377", "title": "Lymphoreticular proliferative disorders of the CNS and other organs: analogies and differences.", "content": "Authors review differences between CNS and extraneural reticuloses, and consider the immediate threat to life by CNS lymphomas contrasting to other organ lymphomas, which usually become fatal only after generalization. Other special features of CNS reticuloses include the role of microglial cells, reactive astrocytosis, variations in meningeal vs. parenchymatous involvement and easier morphologic confusion with inflammatory processes. Analogies exist between CNS and extranodal \"organ\" lymphomas in terms of relatively late or no generalization. The entire spectrum of lymphomatous and histiocytic proliferation may occur in the CNS, the latter including atypical fibrous xanthomas and xanthosarcomas. A further analogy is seen in the presence of lymphocyte and plasma cells in cerebral and organ lymphomas that may well represent host defense reaction. When present in large numbers they may be responsible for the \"inflammatory\" appearance of some reticulum cell sarcomas of the brain.", "contents": "Lymphoreticular proliferative disorders of the CNS and other organs: analogies and differences. Authors review differences between CNS and extraneural reticuloses, and consider the immediate threat to life by CNS lymphomas contrasting to other organ lymphomas, which usually become fatal only after generalization. Other special features of CNS reticuloses include the role of microglial cells, reactive astrocytosis, variations in meningeal vs. parenchymatous involvement and easier morphologic confusion with inflammatory processes. Analogies exist between CNS and extranodal \"organ\" lymphomas in terms of relatively late or no generalization. The entire spectrum of lymphomatous and histiocytic proliferation may occur in the CNS, the latter including atypical fibrous xanthomas and xanthosarcomas. A further analogy is seen in the presence of lymphocyte and plasma cells in cerebral and organ lymphomas that may well represent host defense reaction. When present in large numbers they may be responsible for the \"inflammatory\" appearance of some reticulum cell sarcomas of the brain."}
{"id": "PMID:1098378", "title": "Neoplastic involvement of the CNS in generalized lymphomas.", "content": "In 49 autopsies on patients with generalized malignant lymphomas a thorough histological examination of the CNS was performed. Isolated neoplastic foci were found in the CNS in 10 cases. It is believed that most of the tumour infiltrations in the CNS as a result of neoplastic transformation of local mesenchymal cells and are not blood-borne metastases.", "contents": "Neoplastic involvement of the CNS in generalized lymphomas. In 49 autopsies on patients with generalized malignant lymphomas a thorough histological examination of the CNS was performed. Isolated neoplastic foci were found in the CNS in 10 cases. It is believed that most of the tumour infiltrations in the CNS as a result of neoplastic transformation of local mesenchymal cells and are not blood-borne metastases."}
{"id": "PMID:1098379", "title": "Pathology of primary reticulum cell sarcoma of the human central nervous system.", "content": "Among 6000 consecutive autopsies, 9 cases of primary reticulum cell sarcoma of the central nervous system were discovered. There were 5 males and 4 females from 17 to 68 years old, and the mean clinical duration was 5 months. Gross examination revealed a wide range of lesions in the cerebral hemispheres, cerebellum and spinal cord. Histologically, the same type of malignant reticulum cell proliferation was encountered in every case.", "contents": "Pathology of primary reticulum cell sarcoma of the human central nervous system. Among 6000 consecutive autopsies, 9 cases of primary reticulum cell sarcoma of the central nervous system were discovered. There were 5 males and 4 females from 17 to 68 years old, and the mean clinical duration was 5 months. Gross examination revealed a wide range of lesions in the cerebral hemispheres, cerebellum and spinal cord. Histologically, the same type of malignant reticulum cell proliferation was encountered in every case."}
{"id": "PMID:1098380", "title": "Primary malignant lymphomas of the central nervous system in man.", "content": "Sixty-eight primary malignant lymphomas of the CNS exclusively confined to the brain and its leptomeninges from a series of about 8000 intracranial neoplasms (incidence 0.85%) were examined and classified according to current histopathologic criteria. Average age at onset of symptoms was 55 years, mean duration of illness to time of diagnosis was 3 months. Survival averaged 1,8 months with supportive care, but 17,2 months with surgery, radiation and/or chemotherapy. CSF cytology was useful and reliable tool for clinical diagnosis. The cerebral hemispheres were affected in about 50%, the basal ganglia in 18%, posterior fossa in 10%, while multifocal lesions amounted to 22%. All CNS tumors were of the diffuse type of non-Hodgkin's lymphomas; no follicular (germinal center) lymphomas were observed. Three histological patterns comparable to extraneural lymphomas were distinguished: Immunoblastoma (reticulosarcoma) occurred most frequently (58.8%), lympho-plasmacytoid immunocytoma constituted 28 percent, while lymphoblastic lymphoma occurred least frequently (13.2%). There were no significant differences with regard to onset, location, growth pattern or clinical course except for a much poorer prognosis of lymphoblastic lymphoma. Although there are no definite cytological differences between malignant lymphomas arising in extraneural sites or as primary lesions in the CNS, the latter showed a much greater proportion of phagocyting histiocytes (and microglia) and a frequent occurrence of plasmacytes and their precursors which apparently exceded pure host reaction. The prognostic value of modern classification schemes for CNS lymphomas needs further critical evaluation.", "contents": "Primary malignant lymphomas of the central nervous system in man. Sixty-eight primary malignant lymphomas of the CNS exclusively confined to the brain and its leptomeninges from a series of about 8000 intracranial neoplasms (incidence 0.85%) were examined and classified according to current histopathologic criteria. Average age at onset of symptoms was 55 years, mean duration of illness to time of diagnosis was 3 months. Survival averaged 1,8 months with supportive care, but 17,2 months with surgery, radiation and/or chemotherapy. CSF cytology was useful and reliable tool for clinical diagnosis. The cerebral hemispheres were affected in about 50%, the basal ganglia in 18%, posterior fossa in 10%, while multifocal lesions amounted to 22%. All CNS tumors were of the diffuse type of non-Hodgkin's lymphomas; no follicular (germinal center) lymphomas were observed. Three histological patterns comparable to extraneural lymphomas were distinguished: Immunoblastoma (reticulosarcoma) occurred most frequently (58.8%), lympho-plasmacytoid immunocytoma constituted 28 percent, while lymphoblastic lymphoma occurred least frequently (13.2%). There were no significant differences with regard to onset, location, growth pattern or clinical course except for a much poorer prognosis of lymphoblastic lymphoma. Although there are no definite cytological differences between malignant lymphomas arising in extraneural sites or as primary lesions in the CNS, the latter showed a much greater proportion of phagocyting histiocytes (and microglia) and a frequent occurrence of plasmacytes and their precursors which apparently exceded pure host reaction. The prognostic value of modern classification schemes for CNS lymphomas needs further critical evaluation."}
{"id": "PMID:1098381", "title": "Bacterial flora of the normal conjunctiva. II. Methods of obtaining cultures.", "content": "The methods used for obtaining bacterial cultures from the normal conjunctiva were reviewed. A comparison was made between four methods; a) platinum loop, b)calcium alginate swab c) dry, and d) wet cotton wool swab, using agar (all methods) and serum-bouillon (methods b, c, d) as culture media. The comparisons were based on the isolation rate of Staphylococcus albus and corynebacteria. At the same time, four topical anaesthetics (benoxinate, tetracaine, proparacaine, cocaine) and one vital stain (tetrazolium - alcian blue mixture) instilled into the conjunctiva immediately before taking samples, were studied for their effects on the isolation rate of Staphylococcus albus and corynebacteria. For isolating Staphylococcus albus, method d was found to be superior to the others when the methods were used in the above mentioned order, and when agar was the only culture medium used. On the other hand, after instillation of the above mentioned eyedrops, causing the so-called \"washing-out\" effect (Fahmy et al. 1974), method a was found better than the others, providing the methods were used in the a-b-c-d order and when agar only was used. When considering the growth of Staphylococcus albus in serum boullion as well, methods b, c, and d were found equal in effect, but somewhat superior to a. Corynebacteria showed nearly the same isolation rate with all the methods before as well as after the instillation of eye drops.", "contents": "Bacterial flora of the normal conjunctiva. II. Methods of obtaining cultures. The methods used for obtaining bacterial cultures from the normal conjunctiva were reviewed. A comparison was made between four methods; a) platinum loop, b)calcium alginate swab c) dry, and d) wet cotton wool swab, using agar (all methods) and serum-bouillon (methods b, c, d) as culture media. The comparisons were based on the isolation rate of Staphylococcus albus and corynebacteria. At the same time, four topical anaesthetics (benoxinate, tetracaine, proparacaine, cocaine) and one vital stain (tetrazolium - alcian blue mixture) instilled into the conjunctiva immediately before taking samples, were studied for their effects on the isolation rate of Staphylococcus albus and corynebacteria. For isolating Staphylococcus albus, method d was found to be superior to the others when the methods were used in the above mentioned order, and when agar was the only culture medium used. On the other hand, after instillation of the above mentioned eyedrops, causing the so-called \"washing-out\" effect (Fahmy et al. 1974), method a was found better than the others, providing the methods were used in the a-b-c-d order and when agar only was used. When considering the growth of Staphylococcus albus in serum boullion as well, methods b, c, and d were found equal in effect, but somewhat superior to a. Corynebacteria showed nearly the same isolation rate with all the methods before as well as after the instillation of eye drops."}
{"id": "PMID:1098383", "title": "Asymptomatic bacteriuria in schoolgirls. II. Differences in escherichia coli causing asymptomatic bacteriuria.", "content": "Three hundred and forty-three E. coli strains isolated from the urine of patients with asymptomatic bacteriuria (ABU), symptomatic cystitis, or pyelonephritis were analysed with regard to O group distribution and sensitivity to the bactericidal effect of normal serum. Strains of O groups 1, 2, 4, 6, 7, 16, 18 and 75 were found in 31.3% in ABU, in 58.7% in cystitis and in 79.8% in pyelonephritis. Spontaneous agglutination was noted in 45.2% of ABU, 6.5% of cystitis and 1.7% of pyelonephritis strains. The strains from patients with ABU were significantly more sensitive to the bactericidal effect of normal serum than were those from patients with symptomatic urinary tract infection. In some patients with untreated ABU, changes in the characteristics of the urinary strains isolated were noted. The strains tended to become spontaneously agglutinating as well as more sensitive to the bactericidal activity of normal serum. The strains found in patients with ABU probably had an altered cell wall compared with those found in patients with symptomatic infections such that they produce fewer symptoms and possibly be less virulent.", "contents": "Asymptomatic bacteriuria in schoolgirls. II. Differences in escherichia coli causing asymptomatic bacteriuria. Three hundred and forty-three E. coli strains isolated from the urine of patients with asymptomatic bacteriuria (ABU), symptomatic cystitis, or pyelonephritis were analysed with regard to O group distribution and sensitivity to the bactericidal effect of normal serum. Strains of O groups 1, 2, 4, 6, 7, 16, 18 and 75 were found in 31.3% in ABU, in 58.7% in cystitis and in 79.8% in pyelonephritis. Spontaneous agglutination was noted in 45.2% of ABU, 6.5% of cystitis and 1.7% of pyelonephritis strains. The strains from patients with ABU were significantly more sensitive to the bactericidal effect of normal serum than were those from patients with symptomatic urinary tract infection. In some patients with untreated ABU, changes in the characteristics of the urinary strains isolated were noted. The strains tended to become spontaneously agglutinating as well as more sensitive to the bactericidal activity of normal serum. The strains found in patients with ABU probably had an altered cell wall compared with those found in patients with symptomatic infections such that they produce fewer symptoms and possibly be less virulent."}
{"id": "PMID:1098384", "title": "Asymptomatic bacteriuria in school girls. IV Difficulties of level diagnosis and the possible relation to the character of infecting bacteria.", "content": "As no method for localization of urinary tract infection has been shown to be absolutely reliable, six methods have been run parallelly in a study of 60 school girls with asymptomatic bacteriuria caused by E. coli. A poor correlation was obtained between \"high infection\" indicated by bladder washout test and abnormal findings of C-reactive protein, sedimentation rate, antibody titres or renal concentrating capacity, while findings of parenchymal reduction on the pyelogram and reflux on the urethrocystogram were often found within this group. The low frequency of abnormal findings of C-reactive protein, sedimentation rate and renal concentrating capacity in girls with a \"high infection\" may be explained by the finding that the ABU patients were infected with changed E. coli strains probably not able to cause the usual inflammatory reactions. The patients' bacteria were found to be less efficient in providing antigen for antibody determinations than were standard strains of same O group, indicating a difference between strains from ABU patients and standard strains. From the battery of tests used it seemed that most of the girls had a bladder infection. All tests were normal in 48% of the patients while at least three of the methods were abnormal in 12%.", "contents": "Asymptomatic bacteriuria in school girls. IV Difficulties of level diagnosis and the possible relation to the character of infecting bacteria. As no method for localization of urinary tract infection has been shown to be absolutely reliable, six methods have been run parallelly in a study of 60 school girls with asymptomatic bacteriuria caused by E. coli. A poor correlation was obtained between \"high infection\" indicated by bladder washout test and abnormal findings of C-reactive protein, sedimentation rate, antibody titres or renal concentrating capacity, while findings of parenchymal reduction on the pyelogram and reflux on the urethrocystogram were often found within this group. The low frequency of abnormal findings of C-reactive protein, sedimentation rate and renal concentrating capacity in girls with a \"high infection\" may be explained by the finding that the ABU patients were infected with changed E. coli strains probably not able to cause the usual inflammatory reactions. The patients' bacteria were found to be less efficient in providing antigen for antibody determinations than were standard strains of same O group, indicating a difference between strains from ABU patients and standard strains. From the battery of tests used it seemed that most of the girls had a bladder infection. All tests were normal in 48% of the patients while at least three of the methods were abnormal in 12%."}
{"id": "PMID:1098385", "title": "Pulmonary vascular lesions in chickens following intravenous injections of disintegrated cells of Escherichia coli.", "content": "Intravenous injections of disintegrated cells of Escherichia coli in chickens were almost constantly followed by extensive lesions in pulmonary arteries; the alterations consisted of mural fibrinoid necrosis, sometimes with slight intramural occurrence of mononuclear inflammatory cells and eosinophils. Massive perivascular accumulations of the same cell types were also very common findings. Affected arteries were frequently occluded by precipitates, predominantly consisting of the injected material, which were rapidly replaced by proliferating endothelial cells, resulting in obliterative lesions, where giant cells sometimes occurred. The conclusion was drawn that the arterial lesions could most adequately be categorized as hypersensitivity angiitis.", "contents": "Pulmonary vascular lesions in chickens following intravenous injections of disintegrated cells of Escherichia coli. Intravenous injections of disintegrated cells of Escherichia coli in chickens were almost constantly followed by extensive lesions in pulmonary arteries; the alterations consisted of mural fibrinoid necrosis, sometimes with slight intramural occurrence of mononuclear inflammatory cells and eosinophils. Massive perivascular accumulations of the same cell types were also very common findings. Affected arteries were frequently occluded by precipitates, predominantly consisting of the injected material, which were rapidly replaced by proliferating endothelial cells, resulting in obliterative lesions, where giant cells sometimes occurred. The conclusion was drawn that the arterial lesions could most adequately be categorized as hypersensitivity angiitis."}
{"id": "PMID:1098386", "title": "Influence of thymectomy, transfer of thymus and bone marrow cells and treatment with thymosin on the depressed splenic release of lymphocytes into the blood after irradiation.", "content": "The content of lymphocytes in blood samples from the splenic vein and the splenic artery of guinea pigs was determined and the veno-arterial difference in number of cells was used as measure of the net release of cells from the spleen into the blood. The splenic release of lymphocytes was reduced after whole body irradiation with 300 rad. This reduction could partly be prevented by transfusion of bone marrow cells after irradiation, 10(6) cells being the most effective dose. In thymectomized, irradiated animals the restoration of the splenic release of lymphocytes after irradiation was impaired in comparison with sham-operated animals. In the thymectomized animals the transfusion of 10(6) thymus cells had a restitutive effect on the splenic release of lymphocytes, while transfusion of bone marrow cells or daily treatments with thymosin, alone or combined with transfusion of bone marrow cells, had no such restitutive effect. The results indicate that restoration of the splenic release of lymphocytes into the blood after irradiation is thymus dependent and probably caused by a traffic of lymphocytes from the thymus to the spleen.", "contents": "Influence of thymectomy, transfer of thymus and bone marrow cells and treatment with thymosin on the depressed splenic release of lymphocytes into the blood after irradiation. The content of lymphocytes in blood samples from the splenic vein and the splenic artery of guinea pigs was determined and the veno-arterial difference in number of cells was used as measure of the net release of cells from the spleen into the blood. The splenic release of lymphocytes was reduced after whole body irradiation with 300 rad. This reduction could partly be prevented by transfusion of bone marrow cells after irradiation, 10(6) cells being the most effective dose. In thymectomized, irradiated animals the restoration of the splenic release of lymphocytes after irradiation was impaired in comparison with sham-operated animals. In the thymectomized animals the transfusion of 10(6) thymus cells had a restitutive effect on the splenic release of lymphocytes, while transfusion of bone marrow cells or daily treatments with thymosin, alone or combined with transfusion of bone marrow cells, had no such restitutive effect. The results indicate that restoration of the splenic release of lymphocytes into the blood after irradiation is thymus dependent and probably caused by a traffic of lymphocytes from the thymus to the spleen."}
{"id": "PMID:1098387", "title": "Antihypertensive and hypertensive effects of the kidney. Elucidated by treatment with medullary transplants and with blockade either of the reninangiotensin-system or of the prostaglandin biosynthesis.", "content": "After subcutaneous isotransplantation of renal medulla either from normal donors or from the ischaemic kidney of renal two-kidney hypertensive rats, the blood pressure of the renal two-kidney hypertensive recipients was lowered-but not to normal levels. After i.v. injection of the converting enzyme inhibitor SQ 20,881, the blood pressure was further decreased to, or close to, normal blood pressure. A complete normalization of the blood pressure was obtained by the combined treatment with medullary transplants and infusions of the angiotensin II inhibitor Saralasin. The difference between the effects of these two blockers was probably caused by differences in the doses used. Administration of indomethacin, an inhibitor of the prostaglandin biosynthesis, to renal two-kidney hypertensive rats with or without renomedullary transplants failed to provoke a rise in blood pressure. This indicates that the anti-hypertensive activity of renomedullary transplants is not due to the group of prostaglandins, the  synthesis of which is inhibited by indomethacin, and furthermore that these prostaglandins are not of importance to the blood pressure level in renal two-kidney hypertensive rats.", "contents": "Antihypertensive and hypertensive effects of the kidney. Elucidated by treatment with medullary transplants and with blockade either of the reninangiotensin-system or of the prostaglandin biosynthesis. After subcutaneous isotransplantation of renal medulla either from normal donors or from the ischaemic kidney of renal two-kidney hypertensive rats, the blood pressure of the renal two-kidney hypertensive recipients was lowered-but not to normal levels. After i.v. injection of the converting enzyme inhibitor SQ 20,881, the blood pressure was further decreased to, or close to, normal blood pressure. A complete normalization of the blood pressure was obtained by the combined treatment with medullary transplants and infusions of the angiotensin II inhibitor Saralasin. The difference between the effects of these two blockers was probably caused by differences in the doses used. Administration of indomethacin, an inhibitor of the prostaglandin biosynthesis, to renal two-kidney hypertensive rats with or without renomedullary transplants failed to provoke a rise in blood pressure. This indicates that the anti-hypertensive activity of renomedullary transplants is not due to the group of prostaglandins, the  synthesis of which is inhibited by indomethacin, and furthermore that these prostaglandins are not of importance to the blood pressure level in renal two-kidney hypertensive rats."}
{"id": "PMID:1098382", "title": "Directional preponderance, 1942-1974. A review.", "content": "The subject reviewed is the phenomenon of Directional Preponderance (DP), a term first proposed in 1942 by Fitzgerald & Hallpike for an abnormality in the pattern of the caloric responses found to occur in association with a variety of unilateral lesions, both central and peripheral, of the vestibular system. On the basis of observations carried out upon a series of human subjects, Fitzgerald, Hallpike & Cawthorne (1942) argued that the DP towards the unaffected labyrinth which follows unilateral labyrinthectomy is subserved in part by a unilateral loss of utricular function--a utricular paresis. Support for the utricular paresis hypothesis, as developed in the course of subsequent studies by Hallpike and his co-workers is thought to be provided by a substantial body of evidence, both clinical and experimental, which has since become available. This is reviewed.", "contents": "Directional preponderance, 1942-1974. A review. The subject reviewed is the phenomenon of Directional Preponderance (DP), a term first proposed in 1942 by Fitzgerald & Hallpike for an abnormality in the pattern of the caloric responses found to occur in association with a variety of unilateral lesions, both central and peripheral, of the vestibular system. On the basis of observations carried out upon a series of human subjects, Fitzgerald, Hallpike & Cawthorne (1942) argued that the DP towards the unaffected labyrinth which follows unilateral labyrinthectomy is subserved in part by a unilateral loss of utricular function--a utricular paresis. Support for the utricular paresis hypothesis, as developed in the course of subsequent studies by Hallpike and his co-workers is thought to be provided by a substantial body of evidence, both clinical and experimental, which has since become available. This is reviewed."}
{"id": "PMID:1098388", "title": "For new Escherichia coli o antigens, o158, o159, o160 and o161, and two new h antigens, h53 and h54.", "content": "Four Escherichia coli strains were established as antigenic test strains for four new o groups, o158, o159, o160 and o161. The strains of o158 and o159 were isolated from cases of infantile diarrhoea in Great Britain, while the o160 and o161 strains were isolated from faecal specimens in Arabia. The o161 strain was at the same time designated as test strain of a new H antigen, H54, while a strain belonging to o group 148 was established as antigenic test strain for H53; this strain was also isolated from human faeces in Arabia.", "contents": "For new Escherichia coli o antigens, o158, o159, o160 and o161, and two new h antigens, h53 and h54. Four Escherichia coli strains were established as antigenic test strains for four new o groups, o158, o159, o160 and o161. The strains of o158 and o159 were isolated from cases of infantile diarrhoea in Great Britain, while the o160 and o161 strains were isolated from faecal specimens in Arabia. The o161 strain was at the same time designated as test strain of a new H antigen, H54, while a strain belonging to o group 148 was established as antigenic test strain for H53; this strain was also isolated from human faeces in Arabia."}
{"id": "PMID:1098389", "title": "Two new Escherichia coli o antigens, o162 and o163, and one new h antigen, h56. withdrawal of h antigen h50.", "content": "Three E. coli strains representing strains from faeces of normal persons were established as antigenic test strains of two new O antigens, O162 and O163, and one new H antigen, H56.", "contents": "Two new Escherichia coli o antigens, o162 and o163, and one new h antigen, h56. withdrawal of h antigen h50. Three E. coli strains representing strains from faeces of normal persons were established as antigenic test strains of two new O antigens, O162 and O163, and one new H antigen, H56."}
{"id": "PMID:1098390", "title": "Radioimmunoassay of hepatitis b antigen and antibody in dialysis and transplant patients.", "content": "57 dialysis patients, 29 of whom had been exposed to hepatitis in 1968-69, were re-examined for hepatitis B antigen (HB-Ag) and antibody (HB-Ab) by means of radioimmunoassay (RIA) and counter immunoelectrophoresis (CIE). Infected patients were followed for five years. Compared to CIE, RIA did not detect additional HB-Ag-positive patients, but antibody could persistently be detected in seven out of fifteen patients previously supposed to have escaped infection. tnine surviving HB-Ag-positive transplant patients remained carriers for 50-72 months. None developed clinical signs of chronic hepatic failure.", "contents": "Radioimmunoassay of hepatitis b antigen and antibody in dialysis and transplant patients. 57 dialysis patients, 29 of whom had been exposed to hepatitis in 1968-69, were re-examined for hepatitis B antigen (HB-Ag) and antibody (HB-Ab) by means of radioimmunoassay (RIA) and counter immunoelectrophoresis (CIE). Infected patients were followed for five years. Compared to CIE, RIA did not detect additional HB-Ag-positive patients, but antibody could persistently be detected in seven out of fifteen patients previously supposed to have escaped infection. tnine surviving HB-Ag-positive transplant patients remained carriers for 50-72 months. None developed clinical signs of chronic hepatic failure."}
{"id": "PMID:1098391", "title": "New immunofluorescence method for the identification of group a, b, c, e, and g streptococci.", "content": "An immunofluorescence method for the identification of groups A, B, C, E, F, and G beta-haemolytic streptococci has been developed, using FITC-labelled F(ab')2 fragments of IgG with optimal staining characteristics. The method is useful for the rapid confirmation of beta-haemolytic streptococci in cultures; in a blind comparison with Lancefield grouping, there was a 97 per cent agreement.", "contents": "New immunofluorescence method for the identification of group a, b, c, e, and g streptococci. An immunofluorescence method for the identification of groups A, B, C, E, F, and G beta-haemolytic streptococci has been developed, using FITC-labelled F(ab')2 fragments of IgG with optimal staining characteristics. The method is useful for the rapid confirmation of beta-haemolytic streptococci in cultures; in a blind comparison with Lancefield grouping, there was a 97 per cent agreement."}
{"id": "PMID:1098392", "title": "Microbial growth on agar surfaces studied by incident light differential interference contrast microscopy.", "content": "Microbial surface growth on routine opaque agar media was examined by various incident light microscopical techniques. Only differential interference contrast regularly gave good resolution and contrast. The arrangement of units approaching the size of individual bacteria may be judged by low power dry objectives.", "contents": "Microbial growth on agar surfaces studied by incident light differential interference contrast microscopy. Microbial surface growth on routine opaque agar media was examined by various incident light microscopical techniques. Only differential interference contrast regularly gave good resolution and contrast. The arrangement of units approaching the size of individual bacteria may be judged by low power dry objectives."}
{"id": "PMID:1098393", "title": "Application of the passive haemolysis test for the determination of rubella virus antibodies.", "content": "A passive haemolysis test for the determination of antibodies against rubella virus haemagglutinin is presented. According to this method, the principle of radial immunodiffusion techinque is applied. Rubella haemagglutinin-coated chick erythrocytes in the agarose gel were lysed by the diffusing positive sera of complement at 37 degrees C. The passive haemolysis test was compared with the conventional haemagglutination inhibition method, and the diameter of the haemolysis zones was shown to be a direct measure of the quantity of antibody added to the well. A plot of the log (HI titre) against the zone diameter gives a straight line. There is no need to remove nonspecific haemagglutination inhibitors. However, all serum samples must be inactivated at 56 degrees C for 30 minutes before testing. Tests of 200 serum samples from healthy women showed a good correlation between the haemagglutination inhibition titre and the antibody titre determined by the passive haemolysis technique. Twenty-one samples with a haemagglutination inhibition titre less than 10 were also negative in the passive haemolysis test. With the exception of one, all sera with a positive haemagglutination inhibition titre showed a positive haemolysis reaction. The method was found to be as specific and as sensitive as the haemagglutination inhibition test. In addition, the technique is rapid and simple for quantitative studies of antibodies against rubella virus.", "contents": "Application of the passive haemolysis test for the determination of rubella virus antibodies. A passive haemolysis test for the determination of antibodies against rubella virus haemagglutinin is presented. According to this method, the principle of radial immunodiffusion techinque is applied. Rubella haemagglutinin-coated chick erythrocytes in the agarose gel were lysed by the diffusing positive sera of complement at 37 degrees C. The passive haemolysis test was compared with the conventional haemagglutination inhibition method, and the diameter of the haemolysis zones was shown to be a direct measure of the quantity of antibody added to the well. A plot of the log (HI titre) against the zone diameter gives a straight line. There is no need to remove nonspecific haemagglutination inhibitors. However, all serum samples must be inactivated at 56 degrees C for 30 minutes before testing. Tests of 200 serum samples from healthy women showed a good correlation between the haemagglutination inhibition titre and the antibody titre determined by the passive haemolysis technique. Twenty-one samples with a haemagglutination inhibition titre less than 10 were also negative in the passive haemolysis test. With the exception of one, all sera with a positive haemagglutination inhibition titre showed a positive haemolysis reaction. The method was found to be as specific and as sensitive as the haemagglutination inhibition test. In addition, the technique is rapid and simple for quantitative studies of antibodies against rubella virus."}
{"id": "PMID:1098394", "title": "Common enterobacterial antigen in Yersinia enterocolitica.", "content": "Production of the common enterobacterial antigen (CA) by strains of Yersinia enterocolitica (Y.e.) serotypes 3 and 9 (Winblad), by strains of 5 different Y.e. serogroups (Wauters) and various other bacteria was examined. Antibody against CA was raised by immunization of rabbits with E. coli O 14. Extract prepared from S. typhimurium was used for the sensitization of sheep erythrocytes with CA. Absorption and haemagglutination inhibition experiments showed that CA could be detected in heat extracts from all Y.e. strains examined, and in that from Yersinia pseudotuberculosis. CA was not detected in extracts from Pasteurella multocida, Francisella tularensis, Brucella abortus, Acinetobacter calcoaceticus or Pseudomonas aeruginosa. Anti-CA antibodies could not be demonstrated in serum from rabbits immunized with Y.e. bacteria, but were demonstrated in serum from rabbits immunized with a CA-containing fraction of the Y.e. extract. The possibility of participation of anti-CA antibody in indirect haemagglutination tests for detection of antibody to Y.e. O antigens is emphasized.", "contents": "Common enterobacterial antigen in Yersinia enterocolitica. Production of the common enterobacterial antigen (CA) by strains of Yersinia enterocolitica (Y.e.) serotypes 3 and 9 (Winblad), by strains of 5 different Y.e. serogroups (Wauters) and various other bacteria was examined. Antibody against CA was raised by immunization of rabbits with E. coli O 14. Extract prepared from S. typhimurium was used for the sensitization of sheep erythrocytes with CA. Absorption and haemagglutination inhibition experiments showed that CA could be detected in heat extracts from all Y.e. strains examined, and in that from Yersinia pseudotuberculosis. CA was not detected in extracts from Pasteurella multocida, Francisella tularensis, Brucella abortus, Acinetobacter calcoaceticus or Pseudomonas aeruginosa. Anti-CA antibodies could not be demonstrated in serum from rabbits immunized with Y.e. bacteria, but were demonstrated in serum from rabbits immunized with a CA-containing fraction of the Y.e. extract. The possibility of participation of anti-CA antibody in indirect haemagglutination tests for detection of antibody to Y.e. O antigens is emphasized."}
{"id": "PMID:1098395", "title": "Effect of temperature on optical density of rabbit blood in dye dilution studies.", "content": "The optical desity of rabbit blood increased on cooling. This increase was additive to the deflection produced by indocyanine green at all concentrations measured. If calibration curves were not corrected for any increase in optical density produced by cooling the blood, and error (minimum 11%) was introduced in the estimation of cardiac output. The effect of temperature was investigated with three different densitometers. Its wave-length dependence did not follow the absorption spectrum of oxyhemoglobin.", "contents": "Effect of temperature on optical density of rabbit blood in dye dilution studies. The optical desity of rabbit blood increased on cooling. This increase was additive to the deflection produced by indocyanine green at all concentrations measured. If calibration curves were not corrected for any increase in optical density produced by cooling the blood, and error (minimum 11%) was introduced in the estimation of cardiac output. The effect of temperature was investigated with three different densitometers. Its wave-length dependence did not follow the absorption spectrum of oxyhemoglobin."}
{"id": "PMID:1098396", "title": "The effect of spironolactone (Aldactone) and methyldopa in low and normal renin hypertension.", "content": "The effect on BP of 100 and 200 mg spironolactone/day has been compared with that of methyl-dopa, 750 mg/day, and with combined treatment with both drugs in 32 patients with essential hypertension. The 28 patients who completed the entire investigation were treated for 30 weeks, divided into 4 treatment periods and 4 placebo periods of equal duration. BP did not fall significantly during the initial placebo period, and at the end of each of the intervening placebo periods it rose to pretreatment levels. A signifcant decrease in mean BP was found during the 4 treatment periods. A fall exceeding 14% was registered in 32% of the patients after methyldopa, 750 mg/day, in 50% of the patients after spironolactone, 200 mg/day, in 89% after combined treatment with both drugs, and in 29% after spironolactone, 100 mg/day. Low renin hypertension was found in 9 of the 28 patients. The average decrease in mean BP after sironolactone, 200 mg/day, methyldopa, 750 mg/day, and after combined treatment did not differ significantly between the low and the normal renin group. The rationale for using diuretics such as spironolactone or thiazide as the basic therapy in essential hypertension is discussed. It is concluded that both are useful in the treatment of essential hypertension and might be used alone or in combination.", "contents": "The effect of spironolactone (Aldactone) and methyldopa in low and normal renin hypertension. The effect on BP of 100 and 200 mg spironolactone/day has been compared with that of methyl-dopa, 750 mg/day, and with combined treatment with both drugs in 32 patients with essential hypertension. The 28 patients who completed the entire investigation were treated for 30 weeks, divided into 4 treatment periods and 4 placebo periods of equal duration. BP did not fall significantly during the initial placebo period, and at the end of each of the intervening placebo periods it rose to pretreatment levels. A signifcant decrease in mean BP was found during the 4 treatment periods. A fall exceeding 14% was registered in 32% of the patients after methyldopa, 750 mg/day, in 50% of the patients after spironolactone, 200 mg/day, in 89% after combined treatment with both drugs, and in 29% after spironolactone, 100 mg/day. Low renin hypertension was found in 9 of the 28 patients. The average decrease in mean BP after sironolactone, 200 mg/day, methyldopa, 750 mg/day, and after combined treatment did not differ significantly between the low and the normal renin group. The rationale for using diuretics such as spironolactone or thiazide as the basic therapy in essential hypertension is discussed. It is concluded that both are useful in the treatment of essential hypertension and might be used alone or in combination."}
{"id": "PMID:1098397", "title": "Antihypertensive effect of beta-1 receptor blockade and beta-2-receptor stimulation in essential hypertension.", "content": "The antihypertensive effect of practolol, a beta-1-blocker, alone and in combination with a beta-2-stimulating drug, salbutamol, has been studied in a double-blind cross-over trial on 19 patients. Practolol treatment induced a significant BP reduction, while the addition of salbutamol failed to give any further decrease.", "contents": "Antihypertensive effect of beta-1 receptor blockade and beta-2-receptor stimulation in essential hypertension. The antihypertensive effect of practolol, a beta-1-blocker, alone and in combination with a beta-2-stimulating drug, salbutamol, has been studied in a double-blind cross-over trial on 19 patients. Practolol treatment induced a significant BP reduction, while the addition of salbutamol failed to give any further decrease."}
{"id": "PMID:1098401", "title": "The impact of August Krogh on the insulin treatment of diabetes and our present status.", "content": "The influence of August Krogh on the introduction of insulin preparations in Scandinavia is mentioned, and his outstanding physiological background is outlined. Developments in preparation of insulin depending on the progress in protein-chemistry are summarized. The latest step in the purification of insulin extracted from pork pancreas now allows treatment of diabetics with protamine-pork-insulin without causing formation of insulin-antibodies in at least 90 per cent treated with insulin through several months. In only 3 out of 51 diabetics was it possible to demonstrate insulin-antibodies. This new step may be of great interest in two ways 1) it is now possible to make insulin-estimations in the plasma of insulin-treated cases and 2) it will be possible to find out whether elimination of insulin-antibody-formation will reduce the degree of vascular lesions in diabetics, such lesions being the greatest problem in clinical diabetes.", "contents": "The impact of August Krogh on the insulin treatment of diabetes and our present status. The influence of August Krogh on the introduction of insulin preparations in Scandinavia is mentioned, and his outstanding physiological background is outlined. Developments in preparation of insulin depending on the progress in protein-chemistry are summarized. The latest step in the purification of insulin extracted from pork pancreas now allows treatment of diabetics with protamine-pork-insulin without causing formation of insulin-antibodies in at least 90 per cent treated with insulin through several months. In only 3 out of 51 diabetics was it possible to demonstrate insulin-antibodies. This new step may be of great interest in two ways 1) it is now possible to make insulin-estimations in the plasma of insulin-treated cases and 2) it will be possible to find out whether elimination of insulin-antibody-formation will reduce the degree of vascular lesions in diabetics, such lesions being the greatest problem in clinical diabetes."}
{"id": "PMID:1098402", "title": "Effect of hypothermia and endotoxin on phagocytosis.", "content": "Experiments with colloidal 198Au indicated that gold clearance highly decreased in rabbits cooled to 29-30 degrees C. The capacity of storing colloidal gold decreased especially in the spleen and liver. Endotoxin reduced the gold clearance in normothermic rabbits; the decrease was more marked in hypothermic animals.", "contents": "Effect of hypothermia and endotoxin on phagocytosis. Experiments with colloidal 198Au indicated that gold clearance highly decreased in rabbits cooled to 29-30 degrees C. The capacity of storing colloidal gold decreased especially in the spleen and liver. Endotoxin reduced the gold clearance in normothermic rabbits; the decrease was more marked in hypothermic animals."}
{"id": "PMID:1098403", "title": "Study of Escherichia coli O114 associated with a hospital outbreak.", "content": "Escherichia coli O114 strains isolated from infants in the course of a hospital outbreak of enteritis were identical with type strain 26W in antigenic structure (O114:K90a, 90b), and in immunoelectrophoretic pattern (ORSKOV's group 1Bb) but differed from it in fermenting salicin late (18-22 days) and in phage pattern (group A4); the strains produced colicin and were lysogenic. Pathogenicity of the organism was proved by IgM type antibodies in a patient's serum rising in a titre of 1:128 and by its enterotoxin-producing capacity shown by the rabbit ileal loop assay.", "contents": "Study of Escherichia coli O114 associated with a hospital outbreak. Escherichia coli O114 strains isolated from infants in the course of a hospital outbreak of enteritis were identical with type strain 26W in antigenic structure (O114:K90a, 90b), and in immunoelectrophoretic pattern (ORSKOV's group 1Bb) but differed from it in fermenting salicin late (18-22 days) and in phage pattern (group A4); the strains produced colicin and were lysogenic. Pathogenicity of the organism was proved by IgM type antibodies in a patient's serum rising in a titre of 1:128 and by its enterotoxin-producing capacity shown by the rabbit ileal loop assay."}
{"id": "PMID:1098404", "title": "The mutagenic effect of pesticides on Escherichia coli WP2 try.", "content": "Thirty pesticides commercially available in Hungary and three well-known chemical mutagens were applied to agar plate cultures of her+ and her- derivatives of Escherichia coli WP2 try- strain. After incubation, the plates were examined for a relative increase in reverse mutation number. N-Trichloro methylthio-1,2,3,6-tetrahydrophthalimide, dimethyl-3,2-dichlorovinyl-phosphate and N-trichloro methylthiophyhalamide proved to have definite mutagenic activity.", "contents": "The mutagenic effect of pesticides on Escherichia coli WP2 try. Thirty pesticides commercially available in Hungary and three well-known chemical mutagens were applied to agar plate cultures of her+ and her- derivatives of Escherichia coli WP2 try- strain. After incubation, the plates were examined for a relative increase in reverse mutation number. N-Trichloro methylthio-1,2,3,6-tetrahydrophthalimide, dimethyl-3,2-dichlorovinyl-phosphate and N-trichloro methylthiophyhalamide proved to have definite mutagenic activity."}
{"id": "PMID:1098406", "title": "Macromolecular synthesis during conjugation in yeast.", "content": "Morphological changes and synthesis of DNA, RNA and protein during conjugation of Saccharomyces cerevisiae was studied. It was found that during conjugation distinct cell expansion and increase in the dry mass of the cell takes place. DNA synthesis is inhibited during mating while synthesis of RNA and protein is not affected. Conjugation of temperature sensitive mutant defective in elongation of DNA chain was normal both in permissive and restrictive temperature. Conjugation of mutants defective in the initiation of DNA synthesis or defective in RNA and protein synthesis was inhibited in restrictive temperature. The results obtained with temperature sensitive mutants suggest that RNA and protein synthesis is necessary for mating reaction while DNA synthesis is not required.", "contents": "Macromolecular synthesis during conjugation in yeast. Morphological changes and synthesis of DNA, RNA and protein during conjugation of Saccharomyces cerevisiae was studied. It was found that during conjugation distinct cell expansion and increase in the dry mass of the cell takes place. DNA synthesis is inhibited during mating while synthesis of RNA and protein is not affected. Conjugation of temperature sensitive mutant defective in elongation of DNA chain was normal both in permissive and restrictive temperature. Conjugation of mutants defective in the initiation of DNA synthesis or defective in RNA and protein synthesis was inhibited in restrictive temperature. The results obtained with temperature sensitive mutants suggest that RNA and protein synthesis is necessary for mating reaction while DNA synthesis is not required."}
{"id": "PMID:1098407", "title": "Specificity and origin of the Mod2- mutation which restores respiratory sufficient phenotype in some pet mutants in Saccharomyces cerevisiae.", "content": "It has been found that Mod2- mutation restores respiratory sufficient phenotype in cytochrome b less mutant MB127-20C. Mod2- gene when present in the same haploid cell with mutant gene pet25 causing deficiency in cytochromes a+a3 and b seems to be responsible for reappearance of cytochrome b in the cytochrome spectrum as well partial respiratory activity. However, the restored respiratory activity seems to be inefficient as growth on unfermentable carbon sources is still impossible. Evidences are presented that the Mod2- mutation originates from respiratory sufficient strain 18-27 in which op1 mutation has been induced.", "contents": "Specificity and origin of the Mod2- mutation which restores respiratory sufficient phenotype in some pet mutants in Saccharomyces cerevisiae. It has been found that Mod2- mutation restores respiratory sufficient phenotype in cytochrome b less mutant MB127-20C. Mod2- gene when present in the same haploid cell with mutant gene pet25 causing deficiency in cytochromes a+a3 and b seems to be responsible for reappearance of cytochrome b in the cytochrome spectrum as well partial respiratory activity. However, the restored respiratory activity seems to be inefficient as growth on unfermentable carbon sources is still impossible. Evidences are presented that the Mod2- mutation originates from respiratory sufficient strain 18-27 in which op1 mutation has been induced."}
{"id": "PMID:1098408", "title": "Comparison of mutagenic efficiency of decay of 32P incorporated in E. coli WP-2 and E. coli WP-2S cells.", "content": "32P-labeled Escherichia coli WP-2 and Escherichia coli WP-2S cells were stored at minus 196 degrees. The lethal effect induced by 32P decay was equal in both strains. Lethal efficiency of 32P leads to 32P transmutation in DNA amounted to 0.046. Reversion try leads to try+ were induced with a ten times higher efficiency in UV--sensitive strain WP-2S, as compared with strain WP-2.", "contents": "Comparison of mutagenic efficiency of decay of 32P incorporated in E. coli WP-2 and E. coli WP-2S cells. 32P-labeled Escherichia coli WP-2 and Escherichia coli WP-2S cells were stored at minus 196 degrees. The lethal effect induced by 32P decay was equal in both strains. Lethal efficiency of 32P leads to 32P transmutation in DNA amounted to 0.046. Reversion try leads to try+ were induced with a ten times higher efficiency in UV--sensitive strain WP-2S, as compared with strain WP-2."}
{"id": "PMID:1098411", "title": "Interaction of concanavalin A with the surface of virus-infected cells.", "content": "Infection of untransformed cells with a wide-range of non-oncogenic enveloped viruses causes a significant increase in their susceptibility to agglutination by concanavalin A (Con A). The increased Con A agglutinability of these cells is not caused by an increase in the number of Con A sites on the cell surface but involves alteration in the surface properties of infected cells to allow redistribution of Con A receptors to form \"patches\" following binding of Con A to the cell surface. Similarities between Con A-mediated agglutination of normal cells infected with non-oncogenic viruses and the agglutination response to cells transformed by oncogenic viruses will be reviewed. Finally, the use of Con A as an experimental tool to modify the replication and cytopathogenicity of non-oncogenic viruses grown in mammalian cells will be presented.", "contents": "Interaction of concanavalin A with the surface of virus-infected cells. Infection of untransformed cells with a wide-range of non-oncogenic enveloped viruses causes a significant increase in their susceptibility to agglutination by concanavalin A (Con A). The increased Con A agglutinability of these cells is not caused by an increase in the number of Con A sites on the cell surface but involves alteration in the surface properties of infected cells to allow redistribution of Con A receptors to form \"patches\" following binding of Con A to the cell surface. Similarities between Con A-mediated agglutination of normal cells infected with non-oncogenic viruses and the agglutination response to cells transformed by oncogenic viruses will be reviewed. Finally, the use of Con A as an experimental tool to modify the replication and cytopathogenicity of non-oncogenic viruses grown in mammalian cells will be presented."}
{"id": "PMID:1098412", "title": "Effects of concanavalin A on cellular dynamics and membrane transport.", "content": "How does Con A affect the cellular dynamics and cellular transport of various substances? It has been observed that following a 30 minute incubation with Con A (20-66 mug/ml) the tumor cells (Anaplastic carcinoma Type 15091a), unlike the normal cells, gradually retract their pseudopodia, take on a rounded shape and lose their motility. The maximum rounding of tumor cells takes place during the period of 200-250 min following the withdrawal of Con A from the incubating medium. All of these rounded cells later develop their regular pseudopodia. A partial synchrony of cell division is also observed. Associated with the rounding effect, there is a decrease in the rate of uptake of some specific amino acids by the tumor cells. The maximum reduction in the uptake coincides with the stage of maximum rounding. Con A also affects some of the electrical properties of tumor cells. In addition, the incorporation of radioactive thymidine into the nuclei is reduced to a much larger extent than can be accounted for by the reduction in the rate of entry of thymidine into cytoplasm. The saturation densities of both normal and tumor cells are not significantly altered by Con A. Tumor cells treated with Con A (chronically or acutely) are equally as potent as the untreated cells in yielding solid tumors when the host animals are injected with these cell subcutaneously. In conclusion, it is suggested that while Con A does not revert or destroy this particular line of tumor cells, this lectin has some interesting selective effects on the morphology and transport properties of tumor cells.", "contents": "Effects of concanavalin A on cellular dynamics and membrane transport. How does Con A affect the cellular dynamics and cellular transport of various substances? It has been observed that following a 30 minute incubation with Con A (20-66 mug/ml) the tumor cells (Anaplastic carcinoma Type 15091a), unlike the normal cells, gradually retract their pseudopodia, take on a rounded shape and lose their motility. The maximum rounding of tumor cells takes place during the period of 200-250 min following the withdrawal of Con A from the incubating medium. All of these rounded cells later develop their regular pseudopodia. A partial synchrony of cell division is also observed. Associated with the rounding effect, there is a decrease in the rate of uptake of some specific amino acids by the tumor cells. The maximum reduction in the uptake coincides with the stage of maximum rounding. Con A also affects some of the electrical properties of tumor cells. In addition, the incorporation of radioactive thymidine into the nuclei is reduced to a much larger extent than can be accounted for by the reduction in the rate of entry of thymidine into cytoplasm. The saturation densities of both normal and tumor cells are not significantly altered by Con A. Tumor cells treated with Con A (chronically or acutely) are equally as potent as the untreated cells in yielding solid tumors when the host animals are injected with these cell subcutaneously. In conclusion, it is suggested that while Con A does not revert or destroy this particular line of tumor cells, this lectin has some interesting selective effects on the morphology and transport properties of tumor cells."}
{"id": "PMID:1098413", "title": "Concanavalin A and other lectins in the study of tumor cell surface organization.", "content": "Cell surface structures of two mouse ascites tumors were studied using lectins. The tumors are sublines of the spontaneous mammary adenocarcinoma TA3 in strain A, differing in two main characteristics. Subline TA3-St grows only in syngeneic mice and has high expression of H-2 antigens. Subline TA3-Ha, in contrast, proliferates in all mouse strains, and has low amounts of exposed H-2 antigens. Concanavalin A (Con A), phytohemagglutinin (PHA) and Helix pomatia anti A hemagglutinin (HP) were used in agglutination tests, in binding experiments with 125I-labelled lectins and also in fluorescence studies with FITC-labelled lectins. Con A and PHA agglutinated the TA3-St cells but not the TA3-Ha cells. However, fluorescein-labelled Con A and PHA were bound to all cells (greater than 90%) of both sublines. Moreover both cell types contained an identical number of Con A receptors. The same result was obtained when the number of PHA receptors on the two sublines was compared. HP agglutinated TA3-Ha cells but not TA3-St cells. However, in this case, the difference in agglutinability between the lines was due to the presence or absence of HP receptors. All TA3-Ha cells contained large numbers of HP receptors. In contrast the majority (greater than 90%) of the TA3-St cells lacked HP receptors. (See article.) Trypsin released the HP receptors from TA3-Ha cells, at the same time making these cells agglutinable by both Con A and PHA. We conclude that the Ta3-Ha cells have a trypsin sensitive surface glycoprotein (or glycoproteins) detectable by HP, which is absent on most TA3-St cells. It is possible that this glycoprotein interferes with the agglutinability of TA3-Ha cells by Con A and PHA; whether it is also responsible for the low expression of H-2 alloantigen on this cell remains to be seen.", "contents": "Concanavalin A and other lectins in the study of tumor cell surface organization. Cell surface structures of two mouse ascites tumors were studied using lectins. The tumors are sublines of the spontaneous mammary adenocarcinoma TA3 in strain A, differing in two main characteristics. Subline TA3-St grows only in syngeneic mice and has high expression of H-2 antigens. Subline TA3-Ha, in contrast, proliferates in all mouse strains, and has low amounts of exposed H-2 antigens. Concanavalin A (Con A), phytohemagglutinin (PHA) and Helix pomatia anti A hemagglutinin (HP) were used in agglutination tests, in binding experiments with 125I-labelled lectins and also in fluorescence studies with FITC-labelled lectins. Con A and PHA agglutinated the TA3-St cells but not the TA3-Ha cells. However, fluorescein-labelled Con A and PHA were bound to all cells (greater than 90%) of both sublines. Moreover both cell types contained an identical number of Con A receptors. The same result was obtained when the number of PHA receptors on the two sublines was compared. HP agglutinated TA3-Ha cells but not TA3-St cells. However, in this case, the difference in agglutinability between the lines was due to the presence or absence of HP receptors. All TA3-Ha cells contained large numbers of HP receptors. In contrast the majority (greater than 90%) of the TA3-St cells lacked HP receptors. (See article.) Trypsin released the HP receptors from TA3-Ha cells, at the same time making these cells agglutinable by both Con A and PHA. We conclude that the Ta3-Ha cells have a trypsin sensitive surface glycoprotein (or glycoproteins) detectable by HP, which is absent on most TA3-St cells. It is possible that this glycoprotein interferes with the agglutinability of TA3-Ha cells by Con A and PHA; whether it is also responsible for the low expression of H-2 alloantigen on this cell remains to be seen."}
{"id": "PMID:1098414", "title": "Effect of concanavalin A and phytohemagglutinin on the modification of immunogenicity of canine kidney allografts.", "content": "Mongrel dog kidneys were allografted to unrelated nephrectomized recipients which were then treated with subimmunosuppressive doses of azathioprine (2.5 mg/kg/day). Dog kidneys treated in vitro with perfusates containing concanavalin A (Con A) or phytohemagglutinin-P (PHA) survived as long as 60 days (mean 39.8 +/- 4.3) after transplantation, whereas normal kidneys survived less than 16 days. The optimal prolongation was achieved by perfusing the kidneys with 500 ml Ringer's lactate containing 25 mg/L Con A, 25 4 degrees C. Lesser effects were achieved with higher or lower concentrations of Con A, or with perfusions carried out at 25 degrees C. Most evidence suggests that Con A and PHA bind to cell surfaces and interfere with the perception of the graft antigens by the host.", "contents": "Effect of concanavalin A and phytohemagglutinin on the modification of immunogenicity of canine kidney allografts. Mongrel dog kidneys were allografted to unrelated nephrectomized recipients which were then treated with subimmunosuppressive doses of azathioprine (2.5 mg/kg/day). Dog kidneys treated in vitro with perfusates containing concanavalin A (Con A) or phytohemagglutinin-P (PHA) survived as long as 60 days (mean 39.8 +/- 4.3) after transplantation, whereas normal kidneys survived less than 16 days. The optimal prolongation was achieved by perfusing the kidneys with 500 ml Ringer's lactate containing 25 mg/L Con A, 25 4 degrees C. Lesser effects were achieved with higher or lower concentrations of Con A, or with perfusions carried out at 25 degrees C. Most evidence suggests that Con A and PHA bind to cell surfaces and interfere with the perception of the graft antigens by the host."}
{"id": "PMID:1098415", "title": "Evidence for the activation of 3-methylcholanthrene as a carcinogen in vivo and asa mutagen in vitro by P1 -450 from inbred strains of mice.", "content": "Genetic differences in aromatic hydrocarbon \"responsiveness\" exist among various mouse strains. New formation of cytochrome P1-450 and the induction aryl hydrocarbon (benzo [a ])pyrene) hydroxylase (as well as numerous other monooxygenase activities) appear to be associated ultimately with genes that cosegregate at a small number of genetic loci. By comparing \"responsive\" and \"nonresponsive\" siblings, we can evaluate the susceptibility of each individual to various mutagenic chemicals in vitro or carcinogenic agents in vivo.", "contents": "Evidence for the activation of 3-methylcholanthrene as a carcinogen in vivo and asa mutagen in vitro by P1 -450 from inbred strains of mice. Genetic differences in aromatic hydrocarbon \"responsiveness\" exist among various mouse strains. New formation of cytochrome P1-450 and the induction aryl hydrocarbon (benzo [a ])pyrene) hydroxylase (as well as numerous other monooxygenase activities) appear to be associated ultimately with genes that cosegregate at a small number of genetic loci. By comparing \"responsive\" and \"nonresponsive\" siblings, we can evaluate the susceptibility of each individual to various mutagenic chemicals in vitro or carcinogenic agents in vivo."}
{"id": "PMID:1098420", "title": "Pathology of dendrites in subacute spongiform virus encephalopathies.", "content": "The spongiform changes in the cerebral cortex of scrapie mice and of chimpanzees afflicted with experimental kuru and Creutzfeldt-Jakob disease were examined by electron microscopy. The pathognomonic findings consisted of swelling and vacuolation of neurons, particularly of dendrites. Fusion of swollen cells and processes occurred. The changes were associated with alterations of plasma membranes. Curled fragments of membranes accumulated at points of cell fusion and at the margin of vacuoles within dendrites. The abnormal membranes were wider and more osmiophilic than normal plasma membranes. These findings as well as other data on the nature of the atypical viruses of scrapies, kuru, and Creutzfeldt-Jakob disease indicate that the infectious agents are closely associated with membranes and that alterations of neuronal membranes initiate the spongiform degeneration of neurons.", "contents": "Pathology of dendrites in subacute spongiform virus encephalopathies. The spongiform changes in the cerebral cortex of scrapie mice and of chimpanzees afflicted with experimental kuru and Creutzfeldt-Jakob disease were examined by electron microscopy. The pathognomonic findings consisted of swelling and vacuolation of neurons, particularly of dendrites. Fusion of swollen cells and processes occurred. The changes were associated with alterations of plasma membranes. Curled fragments of membranes accumulated at points of cell fusion and at the margin of vacuoles within dendrites. The abnormal membranes were wider and more osmiophilic than normal plasma membranes. These findings as well as other data on the nature of the atypical viruses of scrapies, kuru, and Creutzfeldt-Jakob disease indicate that the infectious agents are closely associated with membranes and that alterations of neuronal membranes initiate the spongiform degeneration of neurons."}
{"id": "PMID:1098426", "title": "Sutures and iris wound-healing in the baboon.", "content": "This paper reports an experimental study of iris wound-healing and the response of iris tissue in baboons to different suture materials following a planned surgical incision. The wound was sutured with 8-0 virgin silk, 22 mum Ethilon or 7-0 chromacised catgut, or left unsutured. The iris produces a minimal inflammatory response to aseptic trauma characterised by round cell infiltration, clump cell proliferation, pigment cell proliferation from the posterior epithelial surface and proliferation of non-pigmented ciliary epithelium (knots). This response is associated with microscopic hemorrhages in the iris. There does not appear to be any fibrous union of wound edges, instead the wound surfaces become lined with a layer of pigmented cells, probably from the posterior epithelium. Sutures play the dual role of accurately apposing the wound edges and, because there is no fibrous union of iris tissue, of keeping them in apposition. Therefore, they must remain in situ permanently. Catgut is poorly tolerated by the iris and is eventually absorbed. For these two reasons it is unsuitable. Ethilon is the least reactive and virgin silk only slightly more reactive. Both of these are suitable for iris suture. The suture material at present available is not entirely satisfactory. Finer suture material is required to reduce even more the iris response to sutures. Needles, even those supplied with Ethilon or virgin silk, are too large and clumsy. The answer to this problem probably lies in strengthening and sharpening the suture material ends so that they can act as needles.", "contents": "Sutures and iris wound-healing in the baboon. This paper reports an experimental study of iris wound-healing and the response of iris tissue in baboons to different suture materials following a planned surgical incision. The wound was sutured with 8-0 virgin silk, 22 mum Ethilon or 7-0 chromacised catgut, or left unsutured. The iris produces a minimal inflammatory response to aseptic trauma characterised by round cell infiltration, clump cell proliferation, pigment cell proliferation from the posterior epithelial surface and proliferation of non-pigmented ciliary epithelium (knots). This response is associated with microscopic hemorrhages in the iris. There does not appear to be any fibrous union of wound edges, instead the wound surfaces become lined with a layer of pigmented cells, probably from the posterior epithelium. Sutures play the dual role of accurately apposing the wound edges and, because there is no fibrous union of iris tissue, of keeping them in apposition. Therefore, they must remain in situ permanently. Catgut is poorly tolerated by the iris and is eventually absorbed. For these two reasons it is unsuitable. Ethilon is the least reactive and virgin silk only slightly more reactive. Both of these are suitable for iris suture. The suture material at present available is not entirely satisfactory. Finer suture material is required to reduce even more the iris response to sutures. Needles, even those supplied with Ethilon or virgin silk, are too large and clumsy. The answer to this problem probably lies in strengthening and sharpening the suture material ends so that they can act as needles."}
{"id": "PMID:1098429", "title": "Animal experiments on an absorbable synthetic thread made of polyglycolic acid (PGA) in corneal surgery.", "content": "Monofilaments of the absorbable synthetic thread Dexon made from PGA have properties similar to nylon 10-0. Experiments with intra-lamellar implantation of the monofilament in rabbit corneas showed the same good material tolerance as nylon 10-0. Perforating corneal wounds were closed with interrupted and running sutures from PGA monofilaments, respectively. One week post-operatively, increased intraocular pressure caused a wound rupture but not a thread rupture. Two weeks post-operatively an increased intraocular pressure of more than 300 mm Hg could no longer rupture the wound. Slit lamp and histological examinations revealed symptoms of absorption after the second week. According to our findings, PGA monofilaments may be suitable for corneal surgery in patients.", "contents": "Animal experiments on an absorbable synthetic thread made of polyglycolic acid (PGA) in corneal surgery. Monofilaments of the absorbable synthetic thread Dexon made from PGA have properties similar to nylon 10-0. Experiments with intra-lamellar implantation of the monofilament in rabbit corneas showed the same good material tolerance as nylon 10-0. Perforating corneal wounds were closed with interrupted and running sutures from PGA monofilaments, respectively. One week post-operatively, increased intraocular pressure caused a wound rupture but not a thread rupture. Two weeks post-operatively an increased intraocular pressure of more than 300 mm Hg could no longer rupture the wound. Slit lamp and histological examinations revealed symptoms of absorption after the second week. According to our findings, PGA monofilaments may be suitable for corneal surgery in patients."}
{"id": "PMID:1098432", "title": "A new technique for measuring the consistency of faeces: a report on its application to the assessment of Senokotot therapy in the elderly.", "content": "The use of a penetrometer provided a reliable and simple method of measuring stool hardness which enabled the stool softening effect of Senokot medication to be quantified.", "contents": "A new technique for measuring the consistency of faeces: a report on its application to the assessment of Senokotot therapy in the elderly. The use of a penetrometer provided a reliable and simple method of measuring stool hardness which enabled the stool softening effect of Senokot medication to be quantified."}
{"id": "PMID:1098436", "title": "Radioimmunological evidence for early functional activity in chick embryonic alpha cells.", "content": "A radioimmunoassay for glucagon was utilized to investigate early developmental aspects of glucagon synthesis and release in the chick embryo. Immunoreactive glucagon was detected in both the pancreas and blood plasma from the fifth embryonic day onwards. The present data show that the chick embryonic alpha cell has the potential for secretory activity very early in development, and suggest a developmental role for glucagon in hepatic glycogen metabolism.", "contents": "Radioimmunological evidence for early functional activity in chick embryonic alpha cells. A radioimmunoassay for glucagon was utilized to investigate early developmental aspects of glucagon synthesis and release in the chick embryo. Immunoreactive glucagon was detected in both the pancreas and blood plasma from the fifth embryonic day onwards. The present data show that the chick embryonic alpha cell has the potential for secretory activity very early in development, and suggest a developmental role for glucagon in hepatic glycogen metabolism."}
{"id": "PMID:1098437", "title": "Sensitivities of strains of enteropathogenic Escherichia coli to cephalexin and other antibiotics.", "content": "The sensitivity patterns of strains of enteropathogenic Escherichia coli to nine antibiotics were determined. Most strains were sensitive to gentamicin, kanamycin, neomycin, and colistin. Sensitivity to cephalexin was generally greater than sensitivity to ampicillin. Compared with sensitivity patterns of strains isolated in previous years, no significant change in sensitivity patterns of recently isolated strains was detected. All ampicillin-resistant strains destroyed the drug by producing beta-lactamase. The activity of this enzyme against cephalexin was significantly lower than its activity against ampicillin. The role of beta-lactamase, the correlation between its production and resistance to beta-lactamase antibiotics, and the similarity between beta-lactamase produced by EEC and the classified beta-lactamases produced by other enteric bacteria and Escherichia coli, are discussed.", "contents": "Sensitivities of strains of enteropathogenic Escherichia coli to cephalexin and other antibiotics. The sensitivity patterns of strains of enteropathogenic Escherichia coli to nine antibiotics were determined. Most strains were sensitive to gentamicin, kanamycin, neomycin, and colistin. Sensitivity to cephalexin was generally greater than sensitivity to ampicillin. Compared with sensitivity patterns of strains isolated in previous years, no significant change in sensitivity patterns of recently isolated strains was detected. All ampicillin-resistant strains destroyed the drug by producing beta-lactamase. The activity of this enzyme against cephalexin was significantly lower than its activity against ampicillin. The role of beta-lactamase, the correlation between its production and resistance to beta-lactamase antibiotics, and the similarity between beta-lactamase produced by EEC and the classified beta-lactamases produced by other enteric bacteria and Escherichia coli, are discussed."}
{"id": "PMID:1098438", "title": "T-cell lymphoma. Report of a case.", "content": "The morphologic, immunologic, and clinical findings in the case of a patient with a T-cell mediastinal lymphoma ((Sternberg sarcoma) are presented.", "contents": "T-cell lymphoma. Report of a case. The morphologic, immunologic, and clinical findings in the case of a patient with a T-cell mediastinal lymphoma ((Sternberg sarcoma) are presented."}
{"id": "PMID:1098439", "title": "Studies of mitogen-induced lymphocyte transformation by a semi-microtechnic.", "content": "The authors have demonstrated that mitogen-induced lymphocyte transformation can be effectively studied in a semi-microsystem allowing multiple studies from  a reasonably small volume of blood, which makes the technic more feasible for clinical studies of cellular immune mechanisms. The variability among individuals, and from day to day in the same individual, makes repeated, careful kinetic studies necessary for valid data. Moreover, the very wide range of normal responses in man makes the interpretation of cellular hyporesponsiveness difficult in pathologic states and necessitates the use of several mitogen concentrations and sequential kinetic studies to establish the validity of results.", "contents": "Studies of mitogen-induced lymphocyte transformation by a semi-microtechnic. The authors have demonstrated that mitogen-induced lymphocyte transformation can be effectively studied in a semi-microsystem allowing multiple studies from  a reasonably small volume of blood, which makes the technic more feasible for clinical studies of cellular immune mechanisms. The variability among individuals, and from day to day in the same individual, makes repeated, careful kinetic studies necessary for valid data. Moreover, the very wide range of normal responses in man makes the interpretation of cellular hyporesponsiveness difficult in pathologic states and necessitates the use of several mitogen concentrations and sequential kinetic studies to establish the validity of results."}
{"id": "PMID:1098440", "title": "Differential characterization of the \"reticulum cell\" in lymphoreticular neoplasms.", "content": "Differential characterization of the \"reticulum cell\" in lymphoreticular neoplasms. Am J Clin Pathol. 64: 171-179, 1975. The term \"reticulum cell\" is confusing, having been applied to the cells involved in many hematopoietic neoplasms, such as reticulum-cell sarcoma, histiocytic medullary reticulosis, leukemic reticuloendotheliosis, and monocytic or histiocytic leukemias. In histologic sections, even the cells from poorly differentiated extramedullary lesions of chloroma or myeloblastic leukemia have been called \"reticulum cells.\"A combined morphologic and cytochemical approach has been used to study \"reticulum cells\"in smears and tissue sections of neoplasms involving \"histiocytes\" or \"reticulum cells.\"The cytochemical markers are: chloracetate esterase for neutrophilic granulocytes; nonspecific esterase and fluoride-resistant esterase for monocytes and histiocytes (phagocytes); tartrate-resistant acid phosphatase for the reticulum cells of leukemic reticuloendotheliosis; pyronin for the lymphatic reticulum cells (germinal center cells). The morphology of these cells is very well appreciated in smears, and the locations of these marked cells in tissue sections are easily recognized. The use of cytochemical and immunochemical methods and functional studies, in addition to simple morphology, may be useful in subclassification of lymphoreticular neoplasms.", "contents": "Differential characterization of the \"reticulum cell\" in lymphoreticular neoplasms. Differential characterization of the \"reticulum cell\" in lymphoreticular neoplasms. Am J Clin Pathol. 64: 171-179, 1975. The term \"reticulum cell\" is confusing, having been applied to the cells involved in many hematopoietic neoplasms, such as reticulum-cell sarcoma, histiocytic medullary reticulosis, leukemic reticuloendotheliosis, and monocytic or histiocytic leukemias. In histologic sections, even the cells from poorly differentiated extramedullary lesions of chloroma or myeloblastic leukemia have been called \"reticulum cells.\"A combined morphologic and cytochemical approach has been used to study \"reticulum cells\"in smears and tissue sections of neoplasms involving \"histiocytes\" or \"reticulum cells.\"The cytochemical markers are: chloracetate esterase for neutrophilic granulocytes; nonspecific esterase and fluoride-resistant esterase for monocytes and histiocytes (phagocytes); tartrate-resistant acid phosphatase for the reticulum cells of leukemic reticuloendotheliosis; pyronin for the lymphatic reticulum cells (germinal center cells). The morphology of these cells is very well appreciated in smears, and the locations of these marked cells in tissue sections are easily recognized. The use of cytochemical and immunochemical methods and functional studies, in addition to simple morphology, may be useful in subclassification of lymphoreticular neoplasms."}
{"id": "PMID:1098441", "title": "Cutaneous infection due to a rough variant of Mycobacterium marinum.", "content": "Spreading lesions clinically resembling lymphangitic sporotrichosis developed on the right arm and chest of a 60-year-old man with chronic lymphocytic leukemia. Acid-fast bacilli were seen in exudates from lesions and in biopsies, and were cultured from them. The isolant grew initially as a yellowish-orange scotochromogen on Lowenstein-Jensen medium at room temperature and at 35 C., but failed to grow at 37 C. It failed to grow on 7-H-10 medium. On repeated subculturing over a 2-year period it gradually converted to a photochromogen. Histologically, there was ulceration with extensive acute and chronic inflammation with fibrosis. Organisms occurred intracellularly as dense, compact, cigar-like packets resembling lepara bacilli. The appeared to have a predilection for the nucleus. The patient was anergic to PPD S, B, Y and G, and lacked antibodies to BCG phosphoglycolipids. The mycobacteriosis was alleviated by combined INH and ethambutol therapy. The isolant was identified as a rough variant of Mycobacterium marinum. It may have been transmitted by an insect vector.", "contents": "Cutaneous infection due to a rough variant of Mycobacterium marinum. Spreading lesions clinically resembling lymphangitic sporotrichosis developed on the right arm and chest of a 60-year-old man with chronic lymphocytic leukemia. Acid-fast bacilli were seen in exudates from lesions and in biopsies, and were cultured from them. The isolant grew initially as a yellowish-orange scotochromogen on Lowenstein-Jensen medium at room temperature and at 35 C., but failed to grow at 37 C. It failed to grow on 7-H-10 medium. On repeated subculturing over a 2-year period it gradually converted to a photochromogen. Histologically, there was ulceration with extensive acute and chronic inflammation with fibrosis. Organisms occurred intracellularly as dense, compact, cigar-like packets resembling lepara bacilli. The appeared to have a predilection for the nucleus. The patient was anergic to PPD S, B, Y and G, and lacked antibodies to BCG phosphoglycolipids. The mycobacteriosis was alleviated by combined INH and ethambutol therapy. The isolant was identified as a rough variant of Mycobacterium marinum. It may have been transmitted by an insect vector."}
{"id": "PMID:1098435", "title": "Long term treatment of steroid dependent asthmatic patients with beclomethasone dipropionate.", "content": "In the efforts to avoid the side-effects of the peroral cortisone drugs in the treatment of asthma, trials have been made since the early fifties with the administration of steroids in aerosol form applied direct on the bronchial mucosa. Hydrocortisone as well as prednisolone and dexamethasone have been used with a documented effect on the obstruction. However, the drugs were found to be resorbed and thus resulted in suppression of adrenal function and appearance of Cushing syndrome and other well-known steroid side-effects (12). More recently, beclomethasone dipropionate -- a steroid with potent topical activity -- has been used as a pressurised aerosol. In 1972 Morrow Brown et al. (1) as well as Lal et al. (10) reported a good therapeutic effect with this drug and at the same time found evidence for the opinion that its systemic effect, if any, was insignificant. Less ositive results were reported by Choo-Kang et al. (4) and also by Herxheimer (8), who considered beclomethasone spray not to possess any advantages as compared with the earlier steroids. The purpose of the present study was to assess the clinical value of beclomethasone in the treatment of adult patients previously on long-term oral steroids.", "contents": "Long term treatment of steroid dependent asthmatic patients with beclomethasone dipropionate. In the efforts to avoid the side-effects of the peroral cortisone drugs in the treatment of asthma, trials have been made since the early fifties with the administration of steroids in aerosol form applied direct on the bronchial mucosa. Hydrocortisone as well as prednisolone and dexamethasone have been used with a documented effect on the obstruction. However, the drugs were found to be resorbed and thus resulted in suppression of adrenal function and appearance of Cushing syndrome and other well-known steroid side-effects (12). More recently, beclomethasone dipropionate -- a steroid with potent topical activity -- has been used as a pressurised aerosol. In 1972 Morrow Brown et al. (1) as well as Lal et al. (10) reported a good therapeutic effect with this drug and at the same time found evidence for the opinion that its systemic effect, if any, was insignificant. Less ositive results were reported by Choo-Kang et al. (4) and also by Herxheimer (8), who considered beclomethasone spray not to possess any advantages as compared with the earlier steroids. The purpose of the present study was to assess the clinical value of beclomethasone in the treatment of adult patients previously on long-term oral steroids."}
{"id": "PMID:1098446", "title": "Enteroinvasive and enterotoxigenic Escherichia coli. Occurrence in acute diarrhea of infants and children.", "content": "The abilities of Escherichia coli to induce diarrhea by enterotoxin production and by intestinal cell penetration have not been explored simultaneously in children with diarrheal disease. In this study, we investigated 36 infants and children with acute diarrhea and 17 healthy controls. From each patient's rectal swab culture, E coli colonies were tested for enterotoxin production by intragastric inoculation in suckling mice and screened for cell penetration with HEp-2 cells. Colonies showing invasiveness in HEp-2 cells were further tested by inoculation into guinea pig conjunctivae. Salmonella or Shigella strains were isolated from 42% of the patients with diarrhea. Enterotoxin-producing strains of E coli were found in 86% of the diarrhea group and in 41% of controls. Strains with capability to invade epithelial cells were found in 30% of the diarrhea group and in 12% of controls. In seven patients, E coli strains demonstrated both enterotoxin production and invasiveness. In an overall view, considering Salmonella, Shigella, and enteroinvasive or enterotoxigenic E coli, a possible etiologic agent was demonstrated in 94% of infants with diarrheal disease. However, the high frequency of enterotoxigenic strains in healthy controls suggest that in some patients with diarrhea there may be a coincidental carrier state with diarrhea actually due to another cause.", "contents": "Enteroinvasive and enterotoxigenic Escherichia coli. Occurrence in acute diarrhea of infants and children. The abilities of Escherichia coli to induce diarrhea by enterotoxin production and by intestinal cell penetration have not been explored simultaneously in children with diarrheal disease. In this study, we investigated 36 infants and children with acute diarrhea and 17 healthy controls. From each patient's rectal swab culture, E coli colonies were tested for enterotoxin production by intragastric inoculation in suckling mice and screened for cell penetration with HEp-2 cells. Colonies showing invasiveness in HEp-2 cells were further tested by inoculation into guinea pig conjunctivae. Salmonella or Shigella strains were isolated from 42% of the patients with diarrhea. Enterotoxin-producing strains of E coli were found in 86% of the diarrhea group and in 41% of controls. Strains with capability to invade epithelial cells were found in 30% of the diarrhea group and in 12% of controls. In seven patients, E coli strains demonstrated both enterotoxin production and invasiveness. In an overall view, considering Salmonella, Shigella, and enteroinvasive or enterotoxigenic E coli, a possible etiologic agent was demonstrated in 94% of infants with diarrheal disease. However, the high frequency of enterotoxigenic strains in healthy controls suggest that in some patients with diarrhea there may be a coincidental carrier state with diarrhea actually due to another cause."}
{"id": "PMID:1098447", "title": "Osteogenesis imperfecta. Review of the literature with presentation of 29 cases.", "content": "Twelve families with 29 patients showing various patterns of osteogenesis imperfecta are described, along with the genetic, pathological, and clinical features. No abnormal blochemical features were found. It has been shown that the best treatment is prophylactic (such as intramedullary nailingy or supportive. There was no remarkable improvement from any form of medical treatment. These data are compared to those of other authors.", "contents": "Osteogenesis imperfecta. Review of the literature with presentation of 29 cases. Twelve families with 29 patients showing various patterns of osteogenesis imperfecta are described, along with the genetic, pathological, and clinical features. No abnormal blochemical features were found. It has been shown that the best treatment is prophylactic (such as intramedullary nailingy or supportive. There was no remarkable improvement from any form of medical treatment. These data are compared to those of other authors."}
{"id": "PMID:1098448", "title": "Duodenal microflora: a prospective study in pediatric gastrointestinal disorders.", "content": "Culture of the duodenal microflora was performed on 96 infants and children with a variety of gastrointestinal disorders. The resident microflora consists predominantly of oral-type microorganisms such as alpha-Streptococci and Neisseria. A fecal-type microflora was found in all 6 infants with post-surgical diarrhea and temporary monosachcaride intolerance; 4 of 22 patients with protracted diarrhea of infancy, 3 of 22 patients with celiac disease, and none of 32 patients with nonspecific diarrhea or 14 others with miscellaneous disorders. Anaerobic microorganisms were seldom isolated. Only 9% of the duodenal cultures were completely sterile, although a further 17 had a quantitative culture less than 5 times 10(4) microorganisms/ml. Small-bowel contamination with fecal-type microorganisms is associated with stasis and disturbance of the normal peristaltic-clearing mechanism. Duodenal culture is essential in any infant with temporary monosaccharide intolerance. It is occasionally useful in other situations where motility may be disturbed.", "contents": "Duodenal microflora: a prospective study in pediatric gastrointestinal disorders. Culture of the duodenal microflora was performed on 96 infants and children with a variety of gastrointestinal disorders. The resident microflora consists predominantly of oral-type microorganisms such as alpha-Streptococci and Neisseria. A fecal-type microflora was found in all 6 infants with post-surgical diarrhea and temporary monosachcaride intolerance; 4 of 22 patients with protracted diarrhea of infancy, 3 of 22 patients with celiac disease, and none of 32 patients with nonspecific diarrhea or 14 others with miscellaneous disorders. Anaerobic microorganisms were seldom isolated. Only 9% of the duodenal cultures were completely sterile, although a further 17 had a quantitative culture less than 5 times 10(4) microorganisms/ml. Small-bowel contamination with fecal-type microorganisms is associated with stasis and disturbance of the normal peristaltic-clearing mechanism. Duodenal culture is essential in any infant with temporary monosaccharide intolerance. It is occasionally useful in other situations where motility may be disturbed."}
{"id": "PMID:1098449", "title": "A controlled trial of azathioprine in Crohn's disease.", "content": "To determine the efficacy of azathioprine in the treatment of Crohn's disease, a 26-week double-blind trial was performed. 20 patients with Crohn's disease, requiring at least 10 mg of prednisone/day over the 3 months prior to entering the study were randomized into placebo (10 patients) and major criterion of success in the trial. There were 7 relapses in the placebo group (5 patients) and 2 relapses in the azathioprine group (2 patients). Complications including fistulae were not affected by the medications. The mean reduction in steriod dosage in the azathioprine group at the end of the trial (-15.5 mg) was greater than in the placebo group (-6.1 mg). These results suggest that azathioprine may permit reduction or discontinuation of steroids without the worsening of symptoms in some patients who appear to require steroids for control of their symptoms. The clinical features of this \"AZA-responsive subgroup\" remain to be defined.", "contents": "A controlled trial of azathioprine in Crohn's disease. To determine the efficacy of azathioprine in the treatment of Crohn's disease, a 26-week double-blind trial was performed. 20 patients with Crohn's disease, requiring at least 10 mg of prednisone/day over the 3 months prior to entering the study were randomized into placebo (10 patients) and major criterion of success in the trial. There were 7 relapses in the placebo group (5 patients) and 2 relapses in the azathioprine group (2 patients). Complications including fistulae were not affected by the medications. The mean reduction in steriod dosage in the azathioprine group at the end of the trial (-15.5 mg) was greater than in the placebo group (-6.1 mg). These results suggest that azathioprine may permit reduction or discontinuation of steroids without the worsening of symptoms in some patients who appear to require steroids for control of their symptoms. The clinical features of this \"AZA-responsive subgroup\" remain to be defined."}
{"id": "PMID:1098450", "title": "Quantitation of intestinal-tissue layers from their histology.", "content": "A line-sampling technique was applied to microscopic histology of human peroral small-intestinal biopsies to quantitate the relative proportions of the tissue layers. In healthy individuals the villus epithelium formed 41--46%, the crypt epithelium 20--26%, and the lamina propria 34% of the mucosa. In gluten enteropathy the villus epithelium formed 7%, crypt epithelium 43%, and lamina propria 50% of the mucosa. In individuals suffering from chronic diarrhea with malabsorption, the percentages of the tissue components were intermediate between those of healthy individuals and those with gluten enteropathy. The validity of the technique was tested in male and female rats with different intestinal size. In adjacent segments, the actual weight of the scraped mucosa and that of the underlying tissues correlated very well with their corresponding weights estimated from the microscopic histology, using the line-sampling technique. The intestinal-tissue layers quantitated were the villus epithelium, crypt epithelium, the lamina propria + muscularis mucosae+submucosa, and the muscle+serosal coats.", "contents": "Quantitation of intestinal-tissue layers from their histology. A line-sampling technique was applied to microscopic histology of human peroral small-intestinal biopsies to quantitate the relative proportions of the tissue layers. In healthy individuals the villus epithelium formed 41--46%, the crypt epithelium 20--26%, and the lamina propria 34% of the mucosa. In gluten enteropathy the villus epithelium formed 7%, crypt epithelium 43%, and lamina propria 50% of the mucosa. In individuals suffering from chronic diarrhea with malabsorption, the percentages of the tissue components were intermediate between those of healthy individuals and those with gluten enteropathy. The validity of the technique was tested in male and female rats with different intestinal size. In adjacent segments, the actual weight of the scraped mucosa and that of the underlying tissues correlated very well with their corresponding weights estimated from the microscopic histology, using the line-sampling technique. The intestinal-tissue layers quantitated were the villus epithelium, crypt epithelium, the lamina propria + muscularis mucosae+submucosa, and the muscle+serosal coats."}
{"id": "PMID:1098452", "title": "Colonization of intensive care unit patients with gram-negative bacilli.", "content": "A prospective study of 64 patients admitted to a medical intensive care unit and 86 patients admitted to a surgical intensive care unit was done to determine the frequency of pharyngeal, intestinal, and tube site colonization with Gram-negative bacilli. Studies were carried out over a 13-week period. The pharyngeal carrier rate among the surgical patients increased by a total of 34 strains compared to 14 strains among medical patients. Similarly, the intestinal carrier rate increased by 35 strains compared to 12 strains. The increased carriage in surgical patients was related more to the presence of indwelling tubes and colonization of multiple sites in the same patient than to the use of antimicrobial drugs. Pharyngeal and rectal colonization in medical patients was related to antibiotic therapy. Indwelling tubes were used predominately in surgical patients and were a significant reservoir of these organisms.", "contents": "Colonization of intensive care unit patients with gram-negative bacilli. A prospective study of 64 patients admitted to a medical intensive care unit and 86 patients admitted to a surgical intensive care unit was done to determine the frequency of pharyngeal, intestinal, and tube site colonization with Gram-negative bacilli. Studies were carried out over a 13-week period. The pharyngeal carrier rate among the surgical patients increased by a total of 34 strains compared to 14 strains among medical patients. Similarly, the intestinal carrier rate increased by 35 strains compared to 12 strains. The increased carriage in surgical patients was related more to the presence of indwelling tubes and colonization of multiple sites in the same patient than to the use of antimicrobial drugs. Pharyngeal and rectal colonization in medical patients was related to antibiotic therapy. Indwelling tubes were used predominately in surgical patients and were a significant reservoir of these organisms."}
{"id": "PMID:1098456", "title": "Glomerular lesions after renal transplantation.", "content": "Significant changes in glomeruli on light microscopy has been observed in 27 of 109 cadaveric renal allografts which functioned beyond 6 months. Tissue was available for study from all but two allografts. The histologic lesions were classified as follows: recurrent glomeruloneophritis, 9 cases (3 focal scierosis, 2 mesangial immunoglobulin A[IgA] disease, 2 mesangiocapillary glomerulonephritis, 1 dense deposit disease, 1 familial nephritis); de novo glomerulonephritis, 1 case (diffuse proliferative glomerulonephritis with crescents); and glomerular change of uncertain etiology, 17 cases (10 mesangiocapillary, 5 focal scierosis, 1 focal proliferative and 1 mesangial proliferative). These lesions were not distinguishable on light, fluorescent and electron microscopy from those in patients with spontaneous renal disease. All patients with glomerular lesions had proteinuria, and all but 3 had microscopic hematuria. Glomerular lesions were not significantly associated with early clinical rejection episodes or HLA compatibility. Presensitization of HLA antigens was significantly related to the occurence of a nonrecurrent glomerular lesion. Vescoureteral reflux was significantly more frequent in those with glomerular change (14 of 24) than in those without (13 of 48). Glomerular lesions were associated with a higher rate of graft loss due to renal transplant failure; renal function in survivors was significantly worse than in those without glomerular lesions.", "contents": "Glomerular lesions after renal transplantation. Significant changes in glomeruli on light microscopy has been observed in 27 of 109 cadaveric renal allografts which functioned beyond 6 months. Tissue was available for study from all but two allografts. The histologic lesions were classified as follows: recurrent glomeruloneophritis, 9 cases (3 focal scierosis, 2 mesangial immunoglobulin A[IgA] disease, 2 mesangiocapillary glomerulonephritis, 1 dense deposit disease, 1 familial nephritis); de novo glomerulonephritis, 1 case (diffuse proliferative glomerulonephritis with crescents); and glomerular change of uncertain etiology, 17 cases (10 mesangiocapillary, 5 focal scierosis, 1 focal proliferative and 1 mesangial proliferative). These lesions were not distinguishable on light, fluorescent and electron microscopy from those in patients with spontaneous renal disease. All patients with glomerular lesions had proteinuria, and all but 3 had microscopic hematuria. Glomerular lesions were not significantly associated with early clinical rejection episodes or HLA compatibility. Presensitization of HLA antigens was significantly related to the occurence of a nonrecurrent glomerular lesion. Vescoureteral reflux was significantly more frequent in those with glomerular change (14 of 24) than in those without (13 of 48). Glomerular lesions were associated with a higher rate of graft loss due to renal transplant failure; renal function in survivors was significantly worse than in those without glomerular lesions."}
{"id": "PMID:1098457", "title": "Chronic membranoproliferative glomerulonephritis. A patient with recurrent episodes simulating rheumatic fever and acute nephritis.", "content": "A most unusual case of chronic membranoproliferative glomerulonephritis with nodule formation is reported in an elderly man in whom it was possible to document many episodes of acute oilguric and hemorrhagic glomerulonephritis and a syndrome resembling acute rheumatic fever. Severe renal failure necessitating peritoneal dialysis occurred on three occasions. Renal function returned to near preexacerbation levels in each episode. Although there was strong clinical evidence for streptococcal hypersensitivity, convincing laboratory documentation was lacking. The histologic and immunopathologic findings changed comparatively little during the several exacerbations. Impairment in fibrinolytic and coagulation activities by circulating macromolecular complexes is postulated for the induction and perpetuation of the glomerular injury.", "contents": "Chronic membranoproliferative glomerulonephritis. A patient with recurrent episodes simulating rheumatic fever and acute nephritis. A most unusual case of chronic membranoproliferative glomerulonephritis with nodule formation is reported in an elderly man in whom it was possible to document many episodes of acute oilguric and hemorrhagic glomerulonephritis and a syndrome resembling acute rheumatic fever. Severe renal failure necessitating peritoneal dialysis occurred on three occasions. Renal function returned to near preexacerbation levels in each episode. Although there was strong clinical evidence for streptococcal hypersensitivity, convincing laboratory documentation was lacking. The histologic and immunopathologic findings changed comparatively little during the several exacerbations. Impairment in fibrinolytic and coagulation activities by circulating macromolecular complexes is postulated for the induction and perpetuation of the glomerular injury."}
{"id": "PMID:1098458", "title": "Cardiac amyloidosis. Diagnosis by transvenous endomyocardial biopsy.", "content": "Endomyocardial tissue, obtained from two patients presenting with restrictive cardiomyopathies, demonstrated amyloid infiltration. The percutaneous transvenous cardiac biopsy technic, using a modified Konno-Sakakibara cardiac bioptome, was safe and quick. Physical examination and catheterization data may not provide a definite differential diagnosis between restrictive and constrictive myocardial disease. Confirmation by biopsy of the cardiac amyloidosis assisted in providing optimum diagnostic and therapeutic care for these patients.", "contents": "Cardiac amyloidosis. Diagnosis by transvenous endomyocardial biopsy. Endomyocardial tissue, obtained from two patients presenting with restrictive cardiomyopathies, demonstrated amyloid infiltration. The percutaneous transvenous cardiac biopsy technic, using a modified Konno-Sakakibara cardiac bioptome, was safe and quick. Physical examination and catheterization data may not provide a definite differential diagnosis between restrictive and constrictive myocardial disease. Confirmation by biopsy of the cardiac amyloidosis assisted in providing optimum diagnostic and therapeutic care for these patients."}
{"id": "PMID:1098459", "title": "Membranoproliferative glomerulonephritis. Progression from the pure form to the crescentic form with recurrence after transplantation.", "content": "Described are the clinical course and renal morphologic findings in a patient with membranoproliferative glomerulonephritis. intially, the patient had a pure form of membranoproliferative glomerulonephritis but after a 3 year course, it became crescentic. After a renal allograft was performed, membranoproliferative glomerulonephritis recurred within 1 month in a pure form and subsequently developed into the crescentic form. This change occurred in the host kidney as well as in the allograft immediately after immunosuppressive and steroid therapy was discontinued.", "contents": "Membranoproliferative glomerulonephritis. Progression from the pure form to the crescentic form with recurrence after transplantation. Described are the clinical course and renal morphologic findings in a patient with membranoproliferative glomerulonephritis. intially, the patient had a pure form of membranoproliferative glomerulonephritis but after a 3 year course, it became crescentic. After a renal allograft was performed, membranoproliferative glomerulonephritis recurred within 1 month in a pure form and subsequently developed into the crescentic form. This change occurred in the host kidney as well as in the allograft immediately after immunosuppressive and steroid therapy was discontinued."}
{"id": "PMID:1098462", "title": "Repeat Marshall-Marchetti procedure for recurrent stress urinary incontinence.", "content": "Thirty-six patients with recurrent stress incontinence had undergone a total of 109 previous procedures, at least one of which had been a Marshall-Marchetti-Krantz (MMK) operation. At the time of operative dissection, it was apparent that most of these patients had had an \"alleged\" MMK operation with no evidence of adhesion or scarring in the periurethral and vesical neck regions. With the transvesical approach, accurate assessment of the tone and quality of the bladder neck and the proximal urethra is possible. This approach also provides an opportunity to \"see and feel\" the effect of the properly placed periurethral and suspending suture. Thirty-two of 36 patients are continent after repeated suspension augmented by plication and bladder revision", "contents": "Repeat Marshall-Marchetti procedure for recurrent stress urinary incontinence. Thirty-six patients with recurrent stress incontinence had undergone a total of 109 previous procedures, at least one of which had been a Marshall-Marchetti-Krantz (MMK) operation. At the time of operative dissection, it was apparent that most of these patients had had an \"alleged\" MMK operation with no evidence of adhesion or scarring in the periurethral and vesical neck regions. With the transvesical approach, accurate assessment of the tone and quality of the bladder neck and the proximal urethra is possible. This approach also provides an opportunity to \"see and feel\" the effect of the properly placed periurethral and suspending suture. Thirty-two of 36 patients are continent after repeated suspension augmented by plication and bladder revision"}
{"id": "PMID:1098463", "title": "Computer diagnosis of labor progression.", "content": "Abnormal labor has been suspected of being inherently delectorious to the fetus.  In order to explore this problem, clinical factors, labor progress, and fetal monitoring parameters were compared in matched groups of high-risk patients whose fetuses were in theoccipitoposterior (0P) and occipitoanterior postions.  The OP group showed significant excesses of dysfunctional labor aptterns, uterine contraction pattern abnormalities, and late and variabl fetal heart rate decelerations not accounted for by theuse of oxytocin or the presence of cord problems.  In OP labor, lower Apgar scores were associated withasignificant excess of preceding fetal heart rate decelerations independant of operative delivery.  This study suggests that neonatal depression in OP laboris related to intrapartum factors preceding delivery and provides direct support for theconcept that abnormal labor may adversely affect the fetus.  The OP position is an indication for close fetomaternal supervision during labor.", "contents": "Computer diagnosis of labor progression. Abnormal labor has been suspected of being inherently delectorious to the fetus.  In order to explore this problem, clinical factors, labor progress, and fetal monitoring parameters were compared in matched groups of high-risk patients whose fetuses were in theoccipitoposterior (0P) and occipitoanterior postions.  The OP group showed significant excesses of dysfunctional labor aptterns, uterine contraction pattern abnormalities, and late and variabl fetal heart rate decelerations not accounted for by theuse of oxytocin or the presence of cord problems.  In OP labor, lower Apgar scores were associated withasignificant excess of preceding fetal heart rate decelerations independant of operative delivery.  This study suggests that neonatal depression in OP laboris related to intrapartum factors preceding delivery and provides direct support for theconcept that abnormal labor may adversely affect the fetus.  The OP position is an indication for close fetomaternal supervision during labor."}
{"id": "PMID:1098465", "title": "Management of infertility resulting from anovulation.", "content": "Recent methods for induction of ovulation in the woman are described. The only indication for use of these medications is induction of ovulation and pregnancy. In properly selected patients, the success rate is quite high, but treatment has undesirable side effects which occasionally may be severe.", "contents": "Management of infertility resulting from anovulation. Recent methods for induction of ovulation in the woman are described. The only indication for use of these medications is induction of ovulation and pregnancy. In properly selected patients, the success rate is quite high, but treatment has undesirable side effects which occasionally may be severe."}
{"id": "PMID:1098466", "title": "Successful induction of follicular maturation and ovulation by prolonged treatment with LH-releasing hormone in women with anorexia nervosa.", "content": "Four women with anorexia nervosa were treated with synthetic LH-releasing hormone (LRH) in an attempt to induce ovulation. All the women had very low pretreatment levels of gonadotropins and estrogens. Administration of LRH resulted in significant gonadotropin increases. The FSH response to LRH in relation to the LH response was higher than in regularly menstruating women. LRH (500 mug) was administered parenterally three times daily over about 4 weeks. During this period there were no significant effects on mood, eating behavior, weight, or libido. All the women responded with follicular maturation and ovulation to the prolonged LRH treatment.", "contents": "Successful induction of follicular maturation and ovulation by prolonged treatment with LH-releasing hormone in women with anorexia nervosa. Four women with anorexia nervosa were treated with synthetic LH-releasing hormone (LRH) in an attempt to induce ovulation. All the women had very low pretreatment levels of gonadotropins and estrogens. Administration of LRH resulted in significant gonadotropin increases. The FSH response to LRH in relation to the LH response was higher than in regularly menstruating women. LRH (500 mug) was administered parenterally three times daily over about 4 weeks. During this period there were no significant effects on mood, eating behavior, weight, or libido. All the women responded with follicular maturation and ovulation to the prolonged LRH treatment."}
{"id": "PMID:1098461", "title": "Serological reactions to Nocardia antigens.", "content": "Sera from 23 patients with nocardiosis were tested for the presence of precipitating antibodies against an extract of Nocardia asteroides. Sera from nine of 20 patients infected with Nocardia asteroides and one of three sera from patients with Nocardia brasiliensis produced one to three precipitin lines with the antigen. Sera from eight of 55 patients with tuberculosis and 11 of 13 patients with leprosy also contained Nocardia precipitins. None of 33 sera from patients with cryptococcosis, blastomycosis, actinomycosis, histoplasmosis, coccidioidomycosis, mucormycosis or candidiasis contained Norcardia antibodies. None of the sera from ten patients infected with Staphylococcus aureus, ten patients infected with Pseudomonas, or 16 sera from uninfected patients contained Nocardia precipitins.", "contents": "Serological reactions to Nocardia antigens. Sera from 23 patients with nocardiosis were tested for the presence of precipitating antibodies against an extract of Nocardia asteroides. Sera from nine of 20 patients infected with Nocardia asteroides and one of three sera from patients with Nocardia brasiliensis produced one to three precipitin lines with the antigen. Sera from eight of 55 patients with tuberculosis and 11 of 13 patients with leprosy also contained Nocardia precipitins. None of 33 sera from patients with cryptococcosis, blastomycosis, actinomycosis, histoplasmosis, coccidioidomycosis, mucormycosis or candidiasis contained Norcardia antibodies. None of the sera from ten patients infected with Staphylococcus aureus, ten patients infected with Pseudomonas, or 16 sera from uninfected patients contained Nocardia precipitins."}
{"id": "PMID:1098468", "title": "The incidence of retinal detachment after penetrating keratoplasty.", "content": "Of 610 penetrating keratoplasties reviewed, retinal detachments did not occur after any phakic graft, or after aphakic or combined (keratoplasty and cataract extraction) procedures unless these were associated with vitreous manipulation. A 5.4% incidence of retinal detachment occurred after keratoplasty that involved vitrectomy. The resultant detachments had a poor prognosis probably because of delayd diagnosis and difficulties in visualization. Vitreous manipulation, therefore, may be essential to graft clarity but it is hazardous.", "contents": "The incidence of retinal detachment after penetrating keratoplasty. Of 610 penetrating keratoplasties reviewed, retinal detachments did not occur after any phakic graft, or after aphakic or combined (keratoplasty and cataract extraction) procedures unless these were associated with vitreous manipulation. A 5.4% incidence of retinal detachment occurred after keratoplasty that involved vitrectomy. The resultant detachments had a poor prognosis probably because of delayd diagnosis and difficulties in visualization. Vitreous manipulation, therefore, may be essential to graft clarity but it is hazardous."}
{"id": "PMID:1098469", "title": "Keratoplasty wound separations.", "content": "Forty cases of partial or full-thickness corneal wound separtions after penetrating keratoplasty comprised three groups. One group of separations occurred before suture removal, due to technical problems encountered at surgery or increased intraocular pressure, or both. Most separations occurred immediately after suture removal and may be the most preventable. The third group of separations occurred long after suture-removal and may not be associated with obvious trauma. About 50% of all wound separations caused graft failure, but patients with fullthickness wound separations had a worse prognosis for visual acuity recovery. Of those grafts that failed after wound separation and were retransplanted, there is a good chance of visual recovery. In our series, fullthickness wound separations or partial wound gape occurred in about 5.7% of our transplant cases. With delay of suture removal associated with a more adequate clinical means of evaluating wound healing, this number may be decreased.", "contents": "Keratoplasty wound separations. Forty cases of partial or full-thickness corneal wound separtions after penetrating keratoplasty comprised three groups. One group of separations occurred before suture removal, due to technical problems encountered at surgery or increased intraocular pressure, or both. Most separations occurred immediately after suture removal and may be the most preventable. The third group of separations occurred long after suture-removal and may not be associated with obvious trauma. About 50% of all wound separations caused graft failure, but patients with fullthickness wound separations had a worse prognosis for visual acuity recovery. Of those grafts that failed after wound separation and were retransplanted, there is a good chance of visual recovery. In our series, fullthickness wound separations or partial wound gape occurred in about 5.7% of our transplant cases. With delay of suture removal associated with a more adequate clinical means of evaluating wound healing, this number may be decreased."}
{"id": "PMID:1098470", "title": "Coagulation and platelet adhesion-inducing factor in the endothelium of the retinal vessels.", "content": "By means of an immunofluorescent technique, we found factors promoting blood coagulation and platelet adhesion in the intima of retinal vessels. These factors coexisted with other agents causing the opposite process, fibrin dissolution. The components of this vascular hemostatic balance are possibly involved in the thrombotic occlusion of the retinal vessels, their canalization, and in the pathogenesis of diabetic retinopathy.", "contents": "Coagulation and platelet adhesion-inducing factor in the endothelium of the retinal vessels. By means of an immunofluorescent technique, we found factors promoting blood coagulation and platelet adhesion in the intima of retinal vessels. These factors coexisted with other agents causing the opposite process, fibrin dissolution. The components of this vascular hemostatic balance are possibly involved in the thrombotic occlusion of the retinal vessels, their canalization, and in the pathogenesis of diabetic retinopathy."}
{"id": "PMID:1098471", "title": "Computerized axial tomography in the detection of orbital space-occupying lesions.", "content": "Of 41 patients we examined for a suspected primary orbital tumor, 25 patients had evidence of a lesion either histologically, angiographically, or surgically. We examined nine of the 25 patients by means of an EMI-Scanner and 16 by a fine matrix addition (160 times 160 cells), and compared these results to those we received by axial tomography, orbital venography, and B-scan ultrasound. We also compared the absorption values of the recorded tumors. The EMI-Scanner had a diagnostic accuracy of 84%; orbital venography, 84%; axial hypocycloidal tomography, 71%; and ultrasound, 76%. The fine matrix scanner gave a preoperative diagnostic rate of 93% and defined optic nerve abnormalities accurately. However, EMI, scanning should complement existing noninvasive procedures and orbital venography.", "contents": "Computerized axial tomography in the detection of orbital space-occupying lesions. Of 41 patients we examined for a suspected primary orbital tumor, 25 patients had evidence of a lesion either histologically, angiographically, or surgically. We examined nine of the 25 patients by means of an EMI-Scanner and 16 by a fine matrix addition (160 times 160 cells), and compared these results to those we received by axial tomography, orbital venography, and B-scan ultrasound. We also compared the absorption values of the recorded tumors. The EMI-Scanner had a diagnostic accuracy of 84%; orbital venography, 84%; axial hypocycloidal tomography, 71%; and ultrasound, 76%. The fine matrix scanner gave a preoperative diagnostic rate of 93% and defined optic nerve abnormalities accurately. However, EMI, scanning should complement existing noninvasive procedures and orbital venography."}
{"id": "PMID:1098472", "title": "Evaluation of intraocular lens fixation in pseudophakia.", "content": "I developed my own lens designs after analyzing the reasons for failure of previous designs. The iris clip lens and the capsular lens are supported by the iris and by the capsular membrane, respectively, the former having minimal and the latter having nearly no contact at all with intraocular tissues. A comparison between the iris and capsular membrane as support for an intraocular lens favor the capsular membrane.", "contents": "Evaluation of intraocular lens fixation in pseudophakia. I developed my own lens designs after analyzing the reasons for failure of previous designs. The iris clip lens and the capsular lens are supported by the iris and by the capsular membrane, respectively, the former having minimal and the latter having nearly no contact at all with intraocular tissues. A comparison between the iris and capsular membrane as support for an intraocular lens favor the capsular membrane."}
{"id": "PMID:1098473", "title": "Repair of cicatricial entropion of the upper eyelid.", "content": "The disparity in vertical length between the skin muscle lamellae and the tarsoconjunctival lamellae in cicatricial entropion of the upper eyelid secondary to contracture of the transconjunctival layer was corrected by a transconjunctival incision allowing the eyelid to straighten. The resultant defect in the tarsoconjunctival lamellae was corrected by means of a bare scleral graft 20% larger than the defect in the eyelid. The graft was allowed to epithelialize spontaneously and was not covered with mucuous membrane.", "contents": "Repair of cicatricial entropion of the upper eyelid. The disparity in vertical length between the skin muscle lamellae and the tarsoconjunctival lamellae in cicatricial entropion of the upper eyelid secondary to contracture of the transconjunctival layer was corrected by a transconjunctival incision allowing the eyelid to straighten. The resultant defect in the tarsoconjunctival lamellae was corrected by means of a bare scleral graft 20% larger than the defect in the eyelid. The graft was allowed to epithelialize spontaneously and was not covered with mucuous membrane."}
{"id": "PMID:1098475", "title": "Hyperacute renal allograft rejection in the primate. Intrarenal effects of heparin and associated net release of factor VIII activity and kallikrein activation.", "content": "Two groups of specifically presensitized Macaca speciosa monkeys received renal allografts via anastomosis to an indwelling arteriovenous (A-V) shunt. One group was pretreated with heparin (2 mg/kg) intravenously and the other was also treated with constant heparin infusion (1 mg/kg/hr) directly into the renal artery. These studies were performed to evaluate the effects of heparin within the kidney on total and compartmental blood flow, complement (C3) levels, sequestration of formed elements, and activation of the coagulation, fibrinolytic, and kinin-forming systems during the initial 3 hours of hyperacute rejection. The results are compared with those previously reported in unmodified control allografts. Heparin prolonged blood clotting time to infinity, markedly prolonged total renal venous blood flow, and normalized compartmental distribution in both groups despite antibody deposition similar to that in controls. With heparin pretreatment only, initial morphologic injury was much reduced but then progressed rapidly. Marked initial cortical cyanosis with mottling appeared to change constantly and was associated with fluctuations in renal turgor, total blood flow, and sequestration of formed elements, all of which suggested repetitive local cortical arterial spasm and incremental destruction of the grafts. Activation of coagulation Factors II and XII was also revealed and marked net Factor VIII activity was observed in the venous effluent. The latter reflects either formation and release of this factor by the injured kidney, or provides in vivo documentation of the \"hyperactivation\" of Factor VIII by thrombin known to occur in vitro. The addition of intrarenal artery heparin infusion resulted in greater improvement in early total blood flow rates and more uniformly progressive cyanosis and loss of turgor, but the diffuse initial morphologic injury suggested more uniform perfusion of injured areas. Intrarenal consumption of C3 and sequestration of formed elements was similar to that in controls. Paradoxically, prompt activation and consumption of all coagulation factors, plasminogen, and prekallikrein were observed, but formed fibrin was sparse. The exess amount of Factor XIIa present during heparin blockade may have been diverted to production of plasminogen activator and kallikrein formation. The enormous numbers of neutrophils observed within vessels of grafts which showed the greatest kallikrein activation provide the probable in vivo demonstration of the chemotactic properties of kallikrein noted by others in vitro. Heparin-induced platelet aggregation may have played an important role in the failure of these grafts. These studies elucidate the intrarenal effects of heparin during hyperacute rejection and again suggest that vasoconstriction is the most important early determinant of graft failure, as blood flow appeared unrelated to the degree of vascular injury and apparent obstruction. Also, heparin may exer a beneficial effect on blood flow by other than its known action on coagulation.", "contents": "Hyperacute renal allograft rejection in the primate. Intrarenal effects of heparin and associated net release of factor VIII activity and kallikrein activation. Two groups of specifically presensitized Macaca speciosa monkeys received renal allografts via anastomosis to an indwelling arteriovenous (A-V) shunt. One group was pretreated with heparin (2 mg/kg) intravenously and the other was also treated with constant heparin infusion (1 mg/kg/hr) directly into the renal artery. These studies were performed to evaluate the effects of heparin within the kidney on total and compartmental blood flow, complement (C3) levels, sequestration of formed elements, and activation of the coagulation, fibrinolytic, and kinin-forming systems during the initial 3 hours of hyperacute rejection. The results are compared with those previously reported in unmodified control allografts. Heparin prolonged blood clotting time to infinity, markedly prolonged total renal venous blood flow, and normalized compartmental distribution in both groups despite antibody deposition similar to that in controls. With heparin pretreatment only, initial morphologic injury was much reduced but then progressed rapidly. Marked initial cortical cyanosis with mottling appeared to change constantly and was associated with fluctuations in renal turgor, total blood flow, and sequestration of formed elements, all of which suggested repetitive local cortical arterial spasm and incremental destruction of the grafts. Activation of coagulation Factors II and XII was also revealed and marked net Factor VIII activity was observed in the venous effluent. The latter reflects either formation and release of this factor by the injured kidney, or provides in vivo documentation of the \"hyperactivation\" of Factor VIII by thrombin known to occur in vitro. The addition of intrarenal artery heparin infusion resulted in greater improvement in early total blood flow rates and more uniformly progressive cyanosis and loss of turgor, but the diffuse initial morphologic injury suggested more uniform perfusion of injured areas. Intrarenal consumption of C3 and sequestration of formed elements was similar to that in controls. Paradoxically, prompt activation and consumption of all coagulation factors, plasminogen, and prekallikrein were observed, but formed fibrin was sparse. The exess amount of Factor XIIa present during heparin blockade may have been diverted to production of plasminogen activator and kallikrein formation. The enormous numbers of neutrophils observed within vessels of grafts which showed the greatest kallikrein activation provide the probable in vivo demonstration of the chemotactic properties of kallikrein noted by others in vitro. Heparin-induced platelet aggregation may have played an important role in the failure of these grafts. These studies elucidate the intrarenal effects of heparin during hyperacute rejection and again suggest that vasoconstriction is the most important early determinant of graft failure, as blood flow appeared unrelated to the degree of vascular injury and apparent obstruction. Also, heparin may exer a beneficial effect on blood flow by other than its known action on coagulation."}
{"id": "PMID:1098476", "title": "A possible autoimmune parathyroiditis following ozone inhalation. II. A histopathologic, ultrastructural, and immunofluorescent study.", "content": "Histologic, ultrastructural, and immunofluorescent changes in the parathyroid glands of rabbits have been studied after 48 hours of ozone inhalation at a dosage of 0.75 ppm. The frequent changes observed included hyperplastic parathyroiditis followed by capillary proliferation and leukocytic infiltration. The progressive cytologic events consisted of the presence of eosinophilic leukocytes, reticuloendothelial and lymphocytic infiltration, disaggregation of the parenchyma, and interstitial edema. The ultrastructural changes consisted of degeneration of nuclei, atrophy of the mitochondria, dilatation and atrophy of the endoplasmic reticulum of the chief cells of the parathyroid gland, proliferation of the venous limb of the capillary network, and the prominent interstitial elements. The immunofluorescent techniques revealed positive immunologic response. These data suggest that ozone inhalation perhaps triggers an immune reaction which causes inflammatory injury to the parathyroid gland. The possibility that the modified functional chemical groups of the parathyroid gland act as autoantigen is discussed.", "contents": "A possible autoimmune parathyroiditis following ozone inhalation. II. A histopathologic, ultrastructural, and immunofluorescent study. Histologic, ultrastructural, and immunofluorescent changes in the parathyroid glands of rabbits have been studied after 48 hours of ozone inhalation at a dosage of 0.75 ppm. The frequent changes observed included hyperplastic parathyroiditis followed by capillary proliferation and leukocytic infiltration. The progressive cytologic events consisted of the presence of eosinophilic leukocytes, reticuloendothelial and lymphocytic infiltration, disaggregation of the parenchyma, and interstitial edema. The ultrastructural changes consisted of degeneration of nuclei, atrophy of the mitochondria, dilatation and atrophy of the endoplasmic reticulum of the chief cells of the parathyroid gland, proliferation of the venous limb of the capillary network, and the prominent interstitial elements. The immunofluorescent techniques revealed positive immunologic response. These data suggest that ozone inhalation perhaps triggers an immune reaction which causes inflammatory injury to the parathyroid gland. The possibility that the modified functional chemical groups of the parathyroid gland act as autoantigen is discussed."}
{"id": "PMID:1098478", "title": "Comparative anatomy of the larynx in man and the chimpanzee: implications for language in Neanderthal.", "content": "Using the larynges of the newborn human and chimpanzee as models, Lieberman and Crelin ('71) and Lieberman, Crelin and Klatt ('72) have reconstructed the larynx of the \"classic\" Neanderthal La Chapelle aux Saints. The authors used their reconstructed vocal tract to generate linguistic functions which led them to conclude that Neanderthal lacked the ability to produce fully articulate human speech. In this paper, it is shown that their reconstruction of the larynx of Neanderthal is based on a placement of the hyoid bone of La Chapelle which is too high. The reconstructed hyoid bone of La Chalpelle which is too high. The reconstructed hyoid bone has been placed in a position unlike that occupied by hyoid bones of newborn humans, adult humans, stillborn chimpanzees or adult chimpanzees. In any laryngeal reconstruction, the function of swallowing must be taken into account. The ability of the reconstructed Neanderthal to swallow is discussed in light of a comparative analysis of swallowing in man and the chimpanzee. It is concluded that the statement that Neanderthal was less than fully articulate remains unsubstantiated because it rests on a questionable reconstruction of the larynx.", "contents": "Comparative anatomy of the larynx in man and the chimpanzee: implications for language in Neanderthal. Using the larynges of the newborn human and chimpanzee as models, Lieberman and Crelin ('71) and Lieberman, Crelin and Klatt ('72) have reconstructed the larynx of the \"classic\" Neanderthal La Chapelle aux Saints. The authors used their reconstructed vocal tract to generate linguistic functions which led them to conclude that Neanderthal lacked the ability to produce fully articulate human speech. In this paper, it is shown that their reconstruction of the larynx of Neanderthal is based on a placement of the hyoid bone of La Chapelle which is too high. The reconstructed hyoid bone of La Chalpelle which is too high. The reconstructed hyoid bone has been placed in a position unlike that occupied by hyoid bones of newborn humans, adult humans, stillborn chimpanzees or adult chimpanzees. In any laryngeal reconstruction, the function of swallowing must be taken into account. The ability of the reconstructed Neanderthal to swallow is discussed in light of a comparative analysis of swallowing in man and the chimpanzee. It is concluded that the statement that Neanderthal was less than fully articulate remains unsubstantiated because it rests on a questionable reconstruction of the larynx."}
{"id": "PMID:1098479", "title": "Genetic, acclimatizational and anthropometric factors in hand cooling among North and South Chinese.", "content": "The left hands of unacclimatized North Chinese (N=16) and South Chinese (N=13) were exposed to 5 degrees C water for 30 minutes followed by a 10 minute recovery period. Significant differences independent of body composition were found between the two samples on mean 30 minute temperature during immersion, time and temperature of the onset of initial CIVD (cold-induced vasodilation) at 0.01, 0.05 and 0.10 levels respectively. The result suggests the presence of a genetic component in the cold responses of Continental Asian populations and the possibility of the presence of a clinal distribution of cold response in Asia.", "contents": "Genetic, acclimatizational and anthropometric factors in hand cooling among North and South Chinese. The left hands of unacclimatized North Chinese (N=16) and South Chinese (N=13) were exposed to 5 degrees C water for 30 minutes followed by a 10 minute recovery period. Significant differences independent of body composition were found between the two samples on mean 30 minute temperature during immersion, time and temperature of the onset of initial CIVD (cold-induced vasodilation) at 0.01, 0.05 and 0.10 levels respectively. The result suggests the presence of a genetic component in the cold responses of Continental Asian populations and the possibility of the presence of a clinal distribution of cold response in Asia."}
{"id": "PMID:1098480", "title": "Arthritis in the prehistoric Southeastern United States: biological and cultural variables.", "content": "Recent research shows that a bacterial life form, Erysipelothrix insidiosa, can produce rheumatoid arthritis in deer, swine, and dogs, and that a number of animals, including man, birds, and fish, may be infected by the organism. Examination of the archaeological record suggests that both cultural and biological variables may be interrelated in the maintenance of some forms of arthritis over long periods of time in geographically disparate populations. Re-examination of Cherokee folk beliefs concerning arthritis suggests that they had some recognition of this connection, and it also suggests that they had some recognition of this connection, and it also suggests that the term \"magical\" may relate more to the world view of the observer than to any actual inability of preliterate peoples to draw causal relations on the basis of their own intimate knowledge of their environments.", "contents": "Arthritis in the prehistoric Southeastern United States: biological and cultural variables. Recent research shows that a bacterial life form, Erysipelothrix insidiosa, can produce rheumatoid arthritis in deer, swine, and dogs, and that a number of animals, including man, birds, and fish, may be infected by the organism. Examination of the archaeological record suggests that both cultural and biological variables may be interrelated in the maintenance of some forms of arthritis over long periods of time in geographically disparate populations. Re-examination of Cherokee folk beliefs concerning arthritis suggests that they had some recognition of this connection, and it also suggests that they had some recognition of this connection, and it also suggests that the term \"magical\" may relate more to the world view of the observer than to any actual inability of preliterate peoples to draw causal relations on the basis of their own intimate knowledge of their environments."}
{"id": "PMID:1098481", "title": "Cobalt inhibition of insulin secretion and calcium uptake by isolated rat islets.", "content": "Cobalt (Co'++) inhibited glucose-, leucine-, and K'+-induced immunoreactive insulin (IRI)release by isolated rat islets.  This inhibition of the insulinotropic effect of glucose was dose dependent, affected both phases of secretion, was very rapid, and was well reversible.  It exhibited a kinetics similiar to that of Ca'++ omission, and the metal (2.5 mM) prevented Ca'++ reintroduction from restoring a normal rate of IRI release.  Theophylline (2 mM) partially overcame the inhibitory effect of 0.5 mM Co'++, but not that of 2.5 mM.  Glucose-induced secretion was lessmarkedly reduced by 0.5 mM Co'++ in the absence of Mg'++ or in the presence of 7.5 mM Ca'++.  Even at 12.5 mM, the metal did not alter glucose oxidation by theislets.  By contrast, Ca uptake by islet cells was reversibly diminished (55%) in the presence of 1.25 mM Co'++.  Calcium influx (measured after 2.5 min) was aslo reduced by Co'++ to a degree that did not change after longer incubation periods.  It is concluded that Co'++ inhibits IRI release mainly through an antaognistic action on Ca entry in beta cells.", "contents": "Cobalt inhibition of insulin secretion and calcium uptake by isolated rat islets. Cobalt (Co'++) inhibited glucose-, leucine-, and K'+-induced immunoreactive insulin (IRI)release by isolated rat islets.  This inhibition of the insulinotropic effect of glucose was dose dependent, affected both phases of secretion, was very rapid, and was well reversible.  It exhibited a kinetics similiar to that of Ca'++ omission, and the metal (2.5 mM) prevented Ca'++ reintroduction from restoring a normal rate of IRI release.  Theophylline (2 mM) partially overcame the inhibitory effect of 0.5 mM Co'++, but not that of 2.5 mM.  Glucose-induced secretion was lessmarkedly reduced by 0.5 mM Co'++ in the absence of Mg'++ or in the presence of 7.5 mM Ca'++.  Even at 12.5 mM, the metal did not alter glucose oxidation by theislets.  By contrast, Ca uptake by islet cells was reversibly diminished (55%) in the presence of 1.25 mM Co'++.  Calcium influx (measured after 2.5 min) was aslo reduced by Co'++ to a degree that did not change after longer incubation periods.  It is concluded that Co'++ inhibits IRI release mainly through an antaognistic action on Ca entry in beta cells."}
{"id": "PMID:1098482", "title": "Secretin effect on gastrin, gastric secretion in dogs with chronic antral stimulation.", "content": "Antrocolic transposition in four dogs with Heidenhain pouch and gastrojejunostomyresulted in a marked increase in fasting plasma gastrin concentration and sustained highacid secretion, closely related to gastrin levels.  A marked production of pepsin output could not be correlated with plasma gastrin.  Reduction in plasma gastrin concentration was more than twofold less pronounced than the reduction in acid output for 1 and 2 U/kg-h, while the 0.5 U/kg-h no effect was noted.  For both acid output and gastrin concentrations, close correlations were noted between presecretion level and remaining level upon secretin infusion.  Despite the reduction in the secretory volume of the pouch, 0.5 and 1 U/kg-h of secretin induced a 1.5- and 2-fold increase in pepsin output, respectively.  Two untis per kilagram-hour decreased the secretory volume as well as the pepsin output.  If the physiological release of secretin in dogs does not exceed the equivalent of the lowest dose studied, our results would indicate that acid inhibition is a physiological activity of secretin, while the effect on circulating gastrin concentration seems to be phamacological.", "contents": "Secretin effect on gastrin, gastric secretion in dogs with chronic antral stimulation. Antrocolic transposition in four dogs with Heidenhain pouch and gastrojejunostomyresulted in a marked increase in fasting plasma gastrin concentration and sustained highacid secretion, closely related to gastrin levels.  A marked production of pepsin output could not be correlated with plasma gastrin.  Reduction in plasma gastrin concentration was more than twofold less pronounced than the reduction in acid output for 1 and 2 U/kg-h, while the 0.5 U/kg-h no effect was noted.  For both acid output and gastrin concentrations, close correlations were noted between presecretion level and remaining level upon secretin infusion.  Despite the reduction in the secretory volume of the pouch, 0.5 and 1 U/kg-h of secretin induced a 1.5- and 2-fold increase in pepsin output, respectively.  Two untis per kilagram-hour decreased the secretory volume as well as the pepsin output.  If the physiological release of secretin in dogs does not exceed the equivalent of the lowest dose studied, our results would indicate that acid inhibition is a physiological activity of secretin, while the effect on circulating gastrin concentration seems to be phamacological."}
{"id": "PMID:1098483", "title": "Psychiatry and the elderly: an overview.", "content": "The author notes that the elderly in our society have not been provided with treatment, research, and services commensurate with their needs. These needs are reflected in the increasing incidence of psychopathology, suicide, and poverty with increasing age. The psychiatric profession's therapeutic nihilism toward the elderly may reflect unresolved countertransference issues that result in a form of prejudice called \"ageism.\" Many of the conditions labeled \"senility\" are actually manifestations of socioeconomic or medical problems that could be resolved with prompt, appropriate treatment. The author makes several recommendations--the creation of a multidisciplinary nongovernmental commission on mental health and illness of the elderly, reexamination by psychiatrists of their attitudes toward the elderly, and proportionate representation of older individuals in psychiatric services, training, and research.", "contents": "Psychiatry and the elderly: an overview. The author notes that the elderly in our society have not been provided with treatment, research, and services commensurate with their needs. These needs are reflected in the increasing incidence of psychopathology, suicide, and poverty with increasing age. The psychiatric profession's therapeutic nihilism toward the elderly may reflect unresolved countertransference issues that result in a form of prejudice called \"ageism.\" Many of the conditions labeled \"senility\" are actually manifestations of socioeconomic or medical problems that could be resolved with prompt, appropriate treatment. The author makes several recommendations--the creation of a multidisciplinary nongovernmental commission on mental health and illness of the elderly, reexamination by psychiatrists of their attitudes toward the elderly, and proportionate representation of older individuals in psychiatric services, training, and research."}
{"id": "PMID:1098484", "title": "A study of transsexuals seeking gender reassignment.", "content": "The authors report the results. of a computerized psychodiagnostic assessment of 19 males and 1 female seeking sex-reassignment surgery. The most pervasive finding was hysterical personality trend (13 of 20 cases). Twelve patients exhibited psychotic trends: 6 showed psychotic thinking on test scores, and 6 received computer-preferred diagnoses of paranoid or schizoid personality. However, most of these 12 patients were not considered psychotic on interview, and those who had sex-reassignment surgery had good results", "contents": "A study of transsexuals seeking gender reassignment. The authors report the results. of a computerized psychodiagnostic assessment of 19 males and 1 female seeking sex-reassignment surgery. The most pervasive finding was hysterical personality trend (13 of 20 cases). Twelve patients exhibited psychotic trends: 6 showed psychotic thinking on test scores, and 6 received computer-preferred diagnoses of paranoid or schizoid personality. However, most of these 12 patients were not considered psychotic on interview, and those who had sex-reassignment surgery had good results"}
{"id": "PMID:1098489", "title": "Massive development of amebas in the large intestine. Fedor Aleksandrovich Lesh (L\u00f6sch).", "content": "Fedor Aleksandrovich Lesh (L\u00f6sch) 1840-1903. Although L\u00f6sch was a distinguished Russian physician, little has been written about him in English. A graduate of the Medico-chirurgical Academy in St. Petersburg (1863), he defended a doctoral thesis on pulmonary embolism and infarction in 1866, and became an assistant professor of medicine in 1871. From 1872 to 1885 he was a lecturer on diagnosis at the Women's Medical College and a consultant to the Nikolayev Military Hospital. In 1885 he became Professor of Diagnostic Medicine at Kiev University where he remained until his retirement in 1897. In 1870, L\u00f6sch found Balantidium coli in the stools of two patients and made a detailed study of the clinical aspects of balantidiasis. In the same year he described in detail Giardia lamblia and Blastocystis hominis. Altogether, he published some 20 papers on the diagnostic and clinical aspects of various diseases, with special emphasis on the microscopic aspects of coprology. It was in 1875 (exactly 100 years ago) that L\u00f6sch, in this monumental paper, reported the clinical aspects of recurrent intestinal amebiasis, identified and described its cause, Entamoeba histolytica, and produced the disease in an experimental animal. This work on amebiasis was completed while he was assistant professor at St. Petersburg, on the wards of Prof. E. Eichwald. Clinicians will cherish the description of the patient's symptoms; protozoologists must be delighted by the description of the parasite and its motility; experimentalists will be nervous about his luck in infecting one of three dogs; and iconoclasts must derive satisfaction from L\u00f6sch's caution in not automatically assigning a direct causal relationship between the presence of the parasite and the disease.", "contents": "Massive development of amebas in the large intestine. Fedor Aleksandrovich Lesh (L\u00f6sch). Fedor Aleksandrovich Lesh (L\u00f6sch) 1840-1903. Although L\u00f6sch was a distinguished Russian physician, little has been written about him in English. A graduate of the Medico-chirurgical Academy in St. Petersburg (1863), he defended a doctoral thesis on pulmonary embolism and infarction in 1866, and became an assistant professor of medicine in 1871. From 1872 to 1885 he was a lecturer on diagnosis at the Women's Medical College and a consultant to the Nikolayev Military Hospital. In 1885 he became Professor of Diagnostic Medicine at Kiev University where he remained until his retirement in 1897. In 1870, L\u00f6sch found Balantidium coli in the stools of two patients and made a detailed study of the clinical aspects of balantidiasis. In the same year he described in detail Giardia lamblia and Blastocystis hominis. Altogether, he published some 20 papers on the diagnostic and clinical aspects of various diseases, with special emphasis on the microscopic aspects of coprology. It was in 1875 (exactly 100 years ago) that L\u00f6sch, in this monumental paper, reported the clinical aspects of recurrent intestinal amebiasis, identified and described its cause, Entamoeba histolytica, and produced the disease in an experimental animal. This work on amebiasis was completed while he was assistant professor at St. Petersburg, on the wards of Prof. E. Eichwald. Clinicians will cherish the description of the patient's symptoms; protozoologists must be delighted by the description of the parasite and its motility; experimentalists will be nervous about his luck in infecting one of three dogs; and iconoclasts must derive satisfaction from L\u00f6sch's caution in not automatically assigning a direct causal relationship between the presence of the parasite and the disease."}
{"id": "PMID:1098490", "title": "Observations on malaria in Indonesian timor.", "content": "Malaria parasitemias were found in 35% of 520 individuals from a village in Timor, Indonesia. Plasmodium falciparum accounted for 80% of infections. The existence of P. ovale in Timor is reported for the first time. The WHO Standard Field Test for drug resistance did not reveal significant resistance of P. falciparum or P. vivax to chloroquine.", "contents": "Observations on malaria in Indonesian timor. Malaria parasitemias were found in 35% of 520 individuals from a village in Timor, Indonesia. Plasmodium falciparum accounted for 80% of infections. The existence of P. ovale in Timor is reported for the first time. The WHO Standard Field Test for drug resistance did not reveal significant resistance of P. falciparum or P. vivax to chloroquine."}
{"id": "PMID:1098491", "title": "Application of the indirect fluorescent antibody method in a study of malaria endemicity in Mato Grosso, Brazil.", "content": "Four surveys of malaria endemicity were conducted in the Cuiab\u00e1 Sector of Mato Grosso State, Brazil, at 6-month intervals during the period April 1970 to September 1971; during April 1970 a survey was also conducted in the Campo Grande Sector. Over 4,000 dual specimens--blood films for parasite diagnosis and filter paper blood spots for determination of fluorescent antibody (IFA) response--were collected from the general population, including school populations whenever possible. Parasitologic examinations yielded positivity rates ranging from 0.8 to 2.3%. In the Cuiab\u00e1 Sector, sero-positives (larger than or equal to 1:20) ranged from 9.3 to 13.6%; in the survey in the Campo Grande Sector only 4.3% of the specimens were positive. There was an expected increase in IFA response with age in both the proportion of positives and mean maximum titers. In the Cuiab\u00e1 surveys 75 to 91% of the maximum positive responses were to the Plasmodium falciparum antigen, while in Campo Grande only 46% of the maximum titers were for P. falciparum. The wide differences in malaria endemicity observed within the Cuiab\u00e1 Secctor were attributed to differences in climate, geography, and degree of development of the several regions. A study of surveillance reports from 1966 through the survey dates revealed that the high rates of infection seen often resulted from detection activities in the larger cities and probably represented cases imported from endemic areas to the north and west. In order to interpret accurately the endemicity of malaria in an area and, thus, determine the necessity for introduction or continuation of control measures, thorough epidemiologic studies are necessary. The addition of a serologic method to normal surveillance can increase the accuracy of interpretation.", "contents": "Application of the indirect fluorescent antibody method in a study of malaria endemicity in Mato Grosso, Brazil. Four surveys of malaria endemicity were conducted in the Cuiab\u00e1 Sector of Mato Grosso State, Brazil, at 6-month intervals during the period April 1970 to September 1971; during April 1970 a survey was also conducted in the Campo Grande Sector. Over 4,000 dual specimens--blood films for parasite diagnosis and filter paper blood spots for determination of fluorescent antibody (IFA) response--were collected from the general population, including school populations whenever possible. Parasitologic examinations yielded positivity rates ranging from 0.8 to 2.3%. In the Cuiab\u00e1 Sector, sero-positives (larger than or equal to 1:20) ranged from 9.3 to 13.6%; in the survey in the Campo Grande Sector only 4.3% of the specimens were positive. There was an expected increase in IFA response with age in both the proportion of positives and mean maximum titers. In the Cuiab\u00e1 surveys 75 to 91% of the maximum positive responses were to the Plasmodium falciparum antigen, while in Campo Grande only 46% of the maximum titers were for P. falciparum. The wide differences in malaria endemicity observed within the Cuiab\u00e1 Secctor were attributed to differences in climate, geography, and degree of development of the several regions. A study of surveillance reports from 1966 through the survey dates revealed that the high rates of infection seen often resulted from detection activities in the larger cities and probably represented cases imported from endemic areas to the north and west. In order to interpret accurately the endemicity of malaria in an area and, thus, determine the necessity for introduction or continuation of control measures, thorough epidemiologic studies are necessary. The addition of a serologic method to normal surveillance can increase the accuracy of interpretation."}
{"id": "PMID:1098492", "title": "Development of antibodies to Plasmodium vivax as measured by two different serologic techniques.", "content": "Sera from individuals infected with Plasmodium vivax were tested for the presence of malarial antibodies using the indirect fluorescent antibody (IFA) and the indirect hemagglutination (IGA) tests. The primary infection resulted in the conversion of all sera to a positive response in the IFA test, whereas only 50% gave a positive IGA response. There was a direct relationship between the duration of the primary parasitemia and percentage giving positive IGA response. Relapse resulted in high level positive IFA and IGA responses.", "contents": "Development of antibodies to Plasmodium vivax as measured by two different serologic techniques. Sera from individuals infected with Plasmodium vivax were tested for the presence of malarial antibodies using the indirect fluorescent antibody (IFA) and the indirect hemagglutination (IGA) tests. The primary infection resulted in the conversion of all sera to a positive response in the IFA test, whereas only 50% gave a positive IGA response. There was a direct relationship between the duration of the primary parasitemia and percentage giving positive IGA response. Relapse resulted in high level positive IFA and IGA responses."}
{"id": "PMID:1098493", "title": "Pathogenesis of acute avian malaria. IV. Immunologic factors in nephritis of acute Plasmodium gallinaceum infections of chickens.", "content": "Study of urinary waste of chickens with acute Plasmodium gallinaceum malaria indicated that serum proteins of the globulin and albumin classes were passed. Protein extravasation did not begin until globulin-associated serum antigen and its antibody were detected in the blood. Both serum antigen and antibody were found in the wastes for as long as the antigen was present in the blood. Extracts of kidney tissues contained serum proteins that were not present in extracts from normal kidneys, and serum antigen and its antibody were both present. Frozen kidney sections reacted strongly with fluorescein-conjugated antibody to serum antigen, showing diffuse granular immunofluorescence characteristic of immune complex nephritis. Sections from chickens with nephritis induced by injections of malarious plasma also showed deposits of extravasated antigen. The experiments suggested that complexes of serum antigen and antibody served as a permeability factor, or activated other permeability factors and may have caused the glomerulonephritis associated with acute avian malaria. The passing of serum proteins in the urinary wastes after chickens had recovered from acute malaria suggested that persisting damage may have resulted.", "contents": "Pathogenesis of acute avian malaria. IV. Immunologic factors in nephritis of acute Plasmodium gallinaceum infections of chickens. Study of urinary waste of chickens with acute Plasmodium gallinaceum malaria indicated that serum proteins of the globulin and albumin classes were passed. Protein extravasation did not begin until globulin-associated serum antigen and its antibody were detected in the blood. Both serum antigen and antibody were found in the wastes for as long as the antigen was present in the blood. Extracts of kidney tissues contained serum proteins that were not present in extracts from normal kidneys, and serum antigen and its antibody were both present. Frozen kidney sections reacted strongly with fluorescein-conjugated antibody to serum antigen, showing diffuse granular immunofluorescence characteristic of immune complex nephritis. Sections from chickens with nephritis induced by injections of malarious plasma also showed deposits of extravasated antigen. The experiments suggested that complexes of serum antigen and antibody served as a permeability factor, or activated other permeability factors and may have caused the glomerulonephritis associated with acute avian malaria. The passing of serum proteins in the urinary wastes after chickens had recovered from acute malaria suggested that persisting damage may have resulted."}
{"id": "PMID:1098494", "title": "Soil survival of toxoplasma oocysts in Kansas and Costa Rica.", "content": "Toxoplasma oocysts in cat feces were marked with a tracer amount of strontium-85 (85-SR), and were superficially buried simulating the natural disposal of feces by cats. Oocyst infectivity in Costa Rica was followed qualitatively and persisted for 1 year in three shaded sites, two moist, and one relatively dry site. Oocyst infectivity was quantitated in the Kansas deposit over a period of 18 months, including two winters. After initial mixing in soil, the level of infectivity remained fairly stable. Infectivity was recovered, probably from the surface on one Musca, several isopods, and earthworms. These data on persistence of Toxoplasma oocysts in soil support the concept that Toxoplasma infectivity in nature may be increased logarithmically by cats.", "contents": "Soil survival of toxoplasma oocysts in Kansas and Costa Rica. Toxoplasma oocysts in cat feces were marked with a tracer amount of strontium-85 (85-SR), and were superficially buried simulating the natural disposal of feces by cats. Oocyst infectivity in Costa Rica was followed qualitatively and persisted for 1 year in three shaded sites, two moist, and one relatively dry site. Oocyst infectivity was quantitated in the Kansas deposit over a period of 18 months, including two winters. After initial mixing in soil, the level of infectivity remained fairly stable. Infectivity was recovered, probably from the surface on one Musca, several isopods, and earthworms. These data on persistence of Toxoplasma oocysts in soil support the concept that Toxoplasma infectivity in nature may be increased logarithmically by cats."}
{"id": "PMID:1098495", "title": "Rifampin therapy of lepromatous leprosy.", "content": "Patients with borderline-lepromatous (BL) or fully lepromatous (LL) leprosy were treated in the sanitarium for approximately 1 year with oral rifampin (600 mg daily) or with oral dapsone (100 mg daily). They were then treated as outpatients with intramuscular acedapsone (225 mg every 12 weeks) or oral dapsone (50 mg daily). They have now been followed for a total of 28 to 34 months. Death of Mycobacterium leprae during the initial 24 weeks was monitored by mouse inoculation with M. leprae from skin punch biopsy specimens. With rifampin therapy, death of M.leprae occurred rapidly, and viable M. leprae were nearly undetectable by the time the first specimen was taken after the start of treatment, at 4 weeks. With dapsone therapy, death of M. leprae was slower, and in some cases the inoculation results were still positive at 12 weeks. The therapeutic response during the period of outpatient treatment has been satisfactory. The number of dead M. leprae, as measured by the bacterial index in skin smears and the number of acid-fast bacteria in skin specimens, has continued to decrease, and clinical progress has been satisfactory. The measured drug-induced death of M. leprae occurred at about the same rate in BL patients as in LL patients. Disappearance of dead M. leprae from the tissues was much more rapid in BL patients than in LL patients.", "contents": "Rifampin therapy of lepromatous leprosy. Patients with borderline-lepromatous (BL) or fully lepromatous (LL) leprosy were treated in the sanitarium for approximately 1 year with oral rifampin (600 mg daily) or with oral dapsone (100 mg daily). They were then treated as outpatients with intramuscular acedapsone (225 mg every 12 weeks) or oral dapsone (50 mg daily). They have now been followed for a total of 28 to 34 months. Death of Mycobacterium leprae during the initial 24 weeks was monitored by mouse inoculation with M. leprae from skin punch biopsy specimens. With rifampin therapy, death of M.leprae occurred rapidly, and viable M. leprae were nearly undetectable by the time the first specimen was taken after the start of treatment, at 4 weeks. With dapsone therapy, death of M. leprae was slower, and in some cases the inoculation results were still positive at 12 weeks. The therapeutic response during the period of outpatient treatment has been satisfactory. The number of dead M. leprae, as measured by the bacterial index in skin smears and the number of acid-fast bacteria in skin specimens, has continued to decrease, and clinical progress has been satisfactory. The measured drug-induced death of M. leprae occurred at about the same rate in BL patients as in LL patients. Disappearance of dead M. leprae from the tissues was much more rapid in BL patients than in LL patients."}
{"id": "PMID:1098496", "title": "Acedapsone (DADDS) treatment of leprosy patients in the Karimui of Papua New Guinea: status at six years.", "content": "Acedapsone (DADDS), a repository sulfone given by injection five times a year, has been used since 1967 for the treatment of all leprosy patients in the Karimui, an area of diffic-lt access. More than 460 patients have been treated, 336 beginning in November 1967 and continuing through the latest assessment 6 years later. The injections have been well received and they have been administered very regularly. Clinical observations were begun before 1967, as a base-line of assessments was available for the patients whose disease appeared before that time. The response to DADDS therapy has been satisfactory except in 5 of the 28 multibacillary patients in whose smears solid-staining Mycobacterium leprae have reappeared. M. leprae was isolated in mice from three of these patients; one strain has been proven to be completely susceptible to dapsone (DDS), and the other two very probably are. DDS levels in the plasma of these five patients were normal and well above the minimal inhibitory concentration. The most probable explanation is that a few viable M. leprae survived in the presence of inhibitory concentrations of DDS for the 4 to 6 years during which dead bacilli were disintegrating and disappearing from the tissues. The other 23 multibacillary patients responded satisfactorily. The decrease in the number of M. leprae in the skin smears has been most prompt in patients with low initial bacterial loads and in those with borderline lepromatous diagnoses. A high initial bacterial load and a fully lepromatous diagnosis were associated with a slow initial loss of M. leprae in the 1st year, followed by a more rapid loss the next year. All of the multibacillary patients have now been treated by the addition of a 90-day course of rifampicin.", "contents": "Acedapsone (DADDS) treatment of leprosy patients in the Karimui of Papua New Guinea: status at six years. Acedapsone (DADDS), a repository sulfone given by injection five times a year, has been used since 1967 for the treatment of all leprosy patients in the Karimui, an area of diffic-lt access. More than 460 patients have been treated, 336 beginning in November 1967 and continuing through the latest assessment 6 years later. The injections have been well received and they have been administered very regularly. Clinical observations were begun before 1967, as a base-line of assessments was available for the patients whose disease appeared before that time. The response to DADDS therapy has been satisfactory except in 5 of the 28 multibacillary patients in whose smears solid-staining Mycobacterium leprae have reappeared. M. leprae was isolated in mice from three of these patients; one strain has been proven to be completely susceptible to dapsone (DDS), and the other two very probably are. DDS levels in the plasma of these five patients were normal and well above the minimal inhibitory concentration. The most probable explanation is that a few viable M. leprae survived in the presence of inhibitory concentrations of DDS for the 4 to 6 years during which dead bacilli were disintegrating and disappearing from the tissues. The other 23 multibacillary patients responded satisfactorily. The decrease in the number of M. leprae in the skin smears has been most prompt in patients with low initial bacterial loads and in those with borderline lepromatous diagnoses. A high initial bacterial load and a fully lepromatous diagnosis were associated with a slow initial loss of M. leprae in the 1st year, followed by a more rapid loss the next year. All of the multibacillary patients have now been treated by the addition of a 90-day course of rifampicin."}
{"id": "PMID:1098497", "title": "Simian hemorrhagic fever: Studies of coagulation and pathology.", "content": "Simian hemorrhagic fever (SHF) was induced in three species of monkeys (Macaca mulatta, M. radiata and M. fascicularis) using plasma from animals that died with SHF in the 1967 outbreak at the California Primate Research Center. The disease was uniformly fatal in all three species with death occurring by day 5 in M. radiata and M. fascicularis and by day 7 in M. mulatta. Serial studies of hemostasis were consistent with the occurrence of disseminated intravascular coagulation, particularly in the M. mulatta. Studies of pathology were typical of previously reported findings in SHF and support the possibility of intravascular coagulation. The role of intravascular coagulation in the pathogenesis and outcome of SHF remains uncertain but studies of the influence of heparin on the disease are in progress.", "contents": "Simian hemorrhagic fever: Studies of coagulation and pathology. Simian hemorrhagic fever (SHF) was induced in three species of monkeys (Macaca mulatta, M. radiata and M. fascicularis) using plasma from animals that died with SHF in the 1967 outbreak at the California Primate Research Center. The disease was uniformly fatal in all three species with death occurring by day 5 in M. radiata and M. fascicularis and by day 7 in M. mulatta. Serial studies of hemostasis were consistent with the occurrence of disseminated intravascular coagulation, particularly in the M. mulatta. Studies of pathology were typical of previously reported findings in SHF and support the possibility of intravascular coagulation. The role of intravascular coagulation in the pathogenesis and outcome of SHF remains uncertain but studies of the influence of heparin on the disease are in progress."}
{"id": "PMID:1098498", "title": "Natural infections of Anopheles albimanus with Plasmodium in a small malaria focus.", "content": "Entomologic surveys conducted in a small village in an area of known high malaria transmission in El Salvador yielded a high rate of infection in Anopheles albimanus collected inside houses in which cases of malaria had occurred. Of 324 specimens dissected, 12 were found to harbor sporozoites or oocysts. This is in contrast to prior reports of extremely low infection rates in collections of this species from malarious areas, and suggests that under some circumstances A. albimanus does meet the criteria of an effective malaria vector.", "contents": "Natural infections of Anopheles albimanus with Plasmodium in a small malaria focus. Entomologic surveys conducted in a small village in an area of known high malaria transmission in El Salvador yielded a high rate of infection in Anopheles albimanus collected inside houses in which cases of malaria had occurred. Of 324 specimens dissected, 12 were found to harbor sporozoites or oocysts. This is in contrast to prior reports of extremely low infection rates in collections of this species from malarious areas, and suggests that under some circumstances A. albimanus does meet the criteria of an effective malaria vector."}
{"id": "PMID:1098499", "title": "Amodiaquine resistant falciparum malaria in Thailand.", "content": "Amodiaquine cured 38% (13/34) of patients with falciparum malaria in Southeast Thailand. Chloroquine cured 0% (0/13). The cure rates with amodiaquine were the same whether a 1.5 g or 2.0 g course was used. Most patients were resistant to amodiaquine at the RI level and to chloroquine at the RII level. In hospital, amodiaquine cleared parasitemia more frequently than did chloroquine. With the 2.0 g course of amodiaquine, the parasite clearance time was 77 hours; the fever clearance time of 36 hours was low and suggests that amodiaquine does not cause a drug fever. Because of resistance, chloroquine should not be used for falciparum malaria in Thailand. Routine use of amodiaquine is not indicated because more effective drugs are available.", "contents": "Amodiaquine resistant falciparum malaria in Thailand. Amodiaquine cured 38% (13/34) of patients with falciparum malaria in Southeast Thailand. Chloroquine cured 0% (0/13). The cure rates with amodiaquine were the same whether a 1.5 g or 2.0 g course was used. Most patients were resistant to amodiaquine at the RI level and to chloroquine at the RII level. In hospital, amodiaquine cleared parasitemia more frequently than did chloroquine. With the 2.0 g course of amodiaquine, the parasite clearance time was 77 hours; the fever clearance time of 36 hours was low and suggests that amodiaquine does not cause a drug fever. Because of resistance, chloroquine should not be used for falciparum malaria in Thailand. Routine use of amodiaquine is not indicated because more effective drugs are available."}
{"id": "PMID:1098500", "title": "Emergence of La Crosse virus from endemic foci. Fluorescent antibody studies of overwintered Aedes triseriatus.", "content": "The frequency and distribution of La Crosse (LAC) virus overwintering in Aedes triseriatus diapause eggs were studied during 1974 by following 64 tree-hole oviposition sites in four enzootic hardwood forest areas. A direct fluorescent antibody technique, adapted for detection of LAC virus in individual mosquitoes, proved to be a rapid, reliable and economical tool for ascertaining true field infection rates. Virus was found in larvae from each of the four study areas before the seasonal emergence of adults and detected in 10 (0.6%) of 1,698 individually processed adults reared from collected larvae. In one of these study areas, all 12 located tree-holes were enclosed with screen before the seasonal emergence of adults, to ensure that all collected from these sites originated from overwintered eggs. Of 1,280 Aedes triseriatus processed from this area throughout the season, 16 (1.2%) contained virus. Isolates were found in overwintered Aedes triseriatus throughout the summer months, demonstrating the role of these tree-hole sites as foci for both overwintering and continuing summer season source of LAC virus in these forested areas.", "contents": "Emergence of La Crosse virus from endemic foci. Fluorescent antibody studies of overwintered Aedes triseriatus. The frequency and distribution of La Crosse (LAC) virus overwintering in Aedes triseriatus diapause eggs were studied during 1974 by following 64 tree-hole oviposition sites in four enzootic hardwood forest areas. A direct fluorescent antibody technique, adapted for detection of LAC virus in individual mosquitoes, proved to be a rapid, reliable and economical tool for ascertaining true field infection rates. Virus was found in larvae from each of the four study areas before the seasonal emergence of adults and detected in 10 (0.6%) of 1,698 individually processed adults reared from collected larvae. In one of these study areas, all 12 located tree-holes were enclosed with screen before the seasonal emergence of adults, to ensure that all collected from these sites originated from overwintered eggs. Of 1,280 Aedes triseriatus processed from this area throughout the season, 16 (1.2%) contained virus. Isolates were found in overwintered Aedes triseriatus throughout the summer months, demonstrating the role of these tree-hole sites as foci for both overwintering and continuing summer season source of LAC virus in these forested areas."}
{"id": "PMID:1098501", "title": "Simplified technic for orthotopic liver transplantation in the rat.", "content": "A new surgical procedure has been developed to simplify the difficult, complex technic of orthotopic liver transplantation in the rat that we previously described. This procedure eliminates the hepatic artery anastomosis and specifically changes the sequence of anastomoses of the vena cava and portal vein to minimize splanchnic congestion and hepatic ischemia. This has simplified and shortened the operation and has eliminated the need for an extracorporeal portosystemic shunt. Seventy-two per cent operative survival was achieved in fifty-seven orthotopic liver transplantations. This simplified technic of orthotopic liver transplantation in the rat can be applied widely to studies of liver physiology, immunologic rejection, and liver preservation.", "contents": "Simplified technic for orthotopic liver transplantation in the rat. A new surgical procedure has been developed to simplify the difficult, complex technic of orthotopic liver transplantation in the rat that we previously described. This procedure eliminates the hepatic artery anastomosis and specifically changes the sequence of anastomoses of the vena cava and portal vein to minimize splanchnic congestion and hepatic ischemia. This has simplified and shortened the operation and has eliminated the need for an extracorporeal portosystemic shunt. Seventy-two per cent operative survival was achieved in fifty-seven orthotopic liver transplantations. This simplified technic of orthotopic liver transplantation in the rat can be applied widely to studies of liver physiology, immunologic rejection, and liver preservation."}
{"id": "PMID:1098502", "title": "Prolongation of pancreatic allograft survival by 5-fluorouracil.", "content": "Recipient survival in canine pancreatic allotransplantation was significantly prolonged when animals were treated with 5-fluorouracil in combination with low-dose azathioprine. Untreated dogs and those receiving only azathioprine survived for only short periods of time, and pancreatitis was frequently encountered. In contrast, animals treated with 5-fluorouracil were protected from pancreatitis and survived an average of almost three weeks.", "contents": "Prolongation of pancreatic allograft survival by 5-fluorouracil. Recipient survival in canine pancreatic allotransplantation was significantly prolonged when animals were treated with 5-fluorouracil in combination with low-dose azathioprine. Untreated dogs and those receiving only azathioprine survived for only short periods of time, and pancreatitis was frequently encountered. In contrast, animals treated with 5-fluorouracil were protected from pancreatitis and survived an average of almost three weeks."}
{"id": "PMID:1098503", "title": "Biology of infections of split thickness skin grafts.", "content": "The purpose of this study was to identify determinants of split thickness skin graft infection. The bacterial count of the experimental wounds was proportional to the incidence of infection in split thickness skin grafts. When the wound was heavily contaminated with 107 organisms, infection developed under most grafts. Graft take frequently occurred in wounds subjected to a lower level of inoculum. The importance of bacterial counts as a determinant of potential skin graft infection was also suggested by a clinical study. We now routinely use quantitative bacterial counts to identify the granulating wounds that are ready for grafting. The type of organism played no significant role in the development of infection.", "contents": "Biology of infections of split thickness skin grafts. The purpose of this study was to identify determinants of split thickness skin graft infection. The bacterial count of the experimental wounds was proportional to the incidence of infection in split thickness skin grafts. When the wound was heavily contaminated with 107 organisms, infection developed under most grafts. Graft take frequently occurred in wounds subjected to a lower level of inoculum. The importance of bacterial counts as a determinant of potential skin graft infection was also suggested by a clinical study. We now routinely use quantitative bacterial counts to identify the granulating wounds that are ready for grafting. The type of organism played no significant role in the development of infection."}
{"id": "PMID:1098506", "title": "Early detection of acute rejection in renal allografts using radioiodinated autologous fibrinogen.", "content": "131I-labeled autologous fibrinogen was used to detect acute renal allograft rejection in the early postoperative period. Ratios of radioactive counts over transplanted kidneys to those over the heart increased with deposition of radioactive fibrinogen in kidneys undergoing rejection. The test was positive in all instances of acute rejection twelve to twenty-four hours prior to clinical ro biochemical changes. False-positive test results were noted in instances of perinephric hematoma, seroma, and wound abscess and in one patient with urinary tract infection. The test was negative in cases of renal failure secondary to acute tubular necrosis, uric acid nephropathy (in the absence of acute rejection), and chronic rejection. This test is simple, rapid, and practical. It can be performed at the bedside and is free from complications, particularly serum hepatitis.", "contents": "Early detection of acute rejection in renal allografts using radioiodinated autologous fibrinogen. 131I-labeled autologous fibrinogen was used to detect acute renal allograft rejection in the early postoperative period. Ratios of radioactive counts over transplanted kidneys to those over the heart increased with deposition of radioactive fibrinogen in kidneys undergoing rejection. The test was positive in all instances of acute rejection twelve to twenty-four hours prior to clinical ro biochemical changes. False-positive test results were noted in instances of perinephric hematoma, seroma, and wound abscess and in one patient with urinary tract infection. The test was negative in cases of renal failure secondary to acute tubular necrosis, uric acid nephropathy (in the absence of acute rejection), and chronic rejection. This test is simple, rapid, and practical. It can be performed at the bedside and is free from complications, particularly serum hepatitis."}
{"id": "PMID:1098507", "title": "Spectrum of cholangitis.", "content": "Of 402 patients admitted with biliary disease over the last three years, cholangitis has been diagnosed in 36. This represents an 8.8 per cent overal incidence and a 33.8 per cent incidence among patients who have undergone operation or manipulation involving the common duct. Based on this experience, a program of prophylaxis and treatment of cholangitis has been devised with special emphasis on the management of elderly patients in the initial postoperative period.", "contents": "Spectrum of cholangitis. Of 402 patients admitted with biliary disease over the last three years, cholangitis has been diagnosed in 36. This represents an 8.8 per cent overal incidence and a 33.8 per cent incidence among patients who have undergone operation or manipulation involving the common duct. Based on this experience, a program of prophylaxis and treatment of cholangitis has been devised with special emphasis on the management of elderly patients in the initial postoperative period."}
{"id": "PMID:1098508", "title": "Enzymatic dissolution of phytobezoars.", "content": "A review of phytobezoars is presented, emphasizing medical treatment using enzymatic dissolution for bezoar located in the gastric pouch. Clinical and in vitro studies show the efficacy of both papain and cellulase, and it is suggested that, since each acts on a different component of the bezoar, they be administered in combination. There were no complications with this treatment in the patients reported in this series.", "contents": "Enzymatic dissolution of phytobezoars. A review of phytobezoars is presented, emphasizing medical treatment using enzymatic dissolution for bezoar located in the gastric pouch. Clinical and in vitro studies show the efficacy of both papain and cellulase, and it is suggested that, since each acts on a different component of the bezoar, they be administered in combination. There were no complications with this treatment in the patients reported in this series."}
{"id": "PMID:1098511", "title": "[Experimental candida keratitis. Histological, immunological and electron microscopical studies (author's transl)].", "content": "Using histological, immunohistological and electron microscopical methods, it could be demonstrated that the course of experimental Candida albicans keratitis in mice invlves three phases: a traumatically caused alternative phase is followed by a granulocytic exsudative phase and finally by a proliferative phase. The experiments were followed over 41 days. Fungal elements could be detected only during the first 4 days after infection. When topically treated with hydrocortisone, the exsudation of leucocytes was diminished and delayed and the cellular proliferation was reduced considerably in the challenged corneal stroma. A lower number of Candida organisms was found in the cornea when treatment with hydrocortisone was combined with the antifungal drug pimaricin. When treated with pimaricin only however, fungal growth and inflammation were least apparent.", "contents": "[Experimental candida keratitis. Histological, immunological and electron microscopical studies (author's transl)]. Using histological, immunohistological and electron microscopical methods, it could be demonstrated that the course of experimental Candida albicans keratitis in mice invlves three phases: a traumatically caused alternative phase is followed by a granulocytic exsudative phase and finally by a proliferative phase. The experiments were followed over 41 days. Fungal elements could be detected only during the first 4 days after infection. When topically treated with hydrocortisone, the exsudation of leucocytes was diminished and delayed and the cellular proliferation was reduced considerably in the challenged corneal stroma. A lower number of Candida organisms was found in the cornea when treatment with hydrocortisone was combined with the antifungal drug pimaricin. When treated with pimaricin only however, fungal growth and inflammation were least apparent."}
{"id": "PMID:1098515", "title": "Rapid freezing of deep cerebral structures for electron microscopy.", "content": "A method is described for the investigation of deep cerebral structures by freeze-substitution. The head of a mouse is sectioned in a guillotine-like apparatus. The exposed surface is subjected to freeze-substitution. Electron micrographs prepared from this material exhibit under a layer of sheared tissue, a layer of tissue comparable with that observed previously in micrographs of the cerebral and cerebellar cortices subjected to freeze-substitution of their natural surfaces. By varying the plane in which the head is sectioned any structure in the brain can be made accessible to freeze-substitution.", "contents": "Rapid freezing of deep cerebral structures for electron microscopy. A method is described for the investigation of deep cerebral structures by freeze-substitution. The head of a mouse is sectioned in a guillotine-like apparatus. The exposed surface is subjected to freeze-substitution. Electron micrographs prepared from this material exhibit under a layer of sheared tissue, a layer of tissue comparable with that observed previously in micrographs of the cerebral and cerebellar cortices subjected to freeze-substitution of their natural surfaces. By varying the plane in which the head is sectioned any structure in the brain can be made accessible to freeze-substitution."}
{"id": "PMID:1098516", "title": "The influence of function on chondrogenesis at the epiphyseal cartilage of a growing long bone.", "content": "In order to study the behaviour of epiphyseal cartilage in a nonfunctional environment, the third metacarpal bone was transplanted intracerebrally as an isograft between 7-day-old litter-mate rats. Host animals were killed 1, 2, 3, 4, 5, 6 weeks post-operatively, and the cellular kinetics evaluated by means of tritiated thymidine autoradiography. The study was also used to compare the effects of function on chondrogenesis at the epiphyseal cartilage with that previously demonstrated for the condylar cartilage of the mandible (Meikle, '73a). Transplantation resulted in three major changes in the cartilage; there was a decreased rate of proliferative activity in the cell columns; the cartilage failed to maintain a satisfactory increase in transverse diameter; the cells of the perichondrium differentiated into osteoblasts instead of chondroblasts. Autoradiographic and histological findings suggested that the inability of the cartilage to increase in transverse diameter was related to the decreased rate of proliferative activity in the cell columns and not to the cessation of perichondrial chondrogenesis. On the basis of these findings two conclusions can be made. 1. Extrinsic mechanical stresses associated with function appear to be necessary for the normal interstitial growth of epiphyseal cartilage during postnatal development, suggesting that functional activity can influence the rate of cell proliferation. 2. Functional activity provides the stimulus for the differentiation of perichondrial cells into chondroblasts.", "contents": "The influence of function on chondrogenesis at the epiphyseal cartilage of a growing long bone. In order to study the behaviour of epiphyseal cartilage in a nonfunctional environment, the third metacarpal bone was transplanted intracerebrally as an isograft between 7-day-old litter-mate rats. Host animals were killed 1, 2, 3, 4, 5, 6 weeks post-operatively, and the cellular kinetics evaluated by means of tritiated thymidine autoradiography. The study was also used to compare the effects of function on chondrogenesis at the epiphyseal cartilage with that previously demonstrated for the condylar cartilage of the mandible (Meikle, '73a). Transplantation resulted in three major changes in the cartilage; there was a decreased rate of proliferative activity in the cell columns; the cartilage failed to maintain a satisfactory increase in transverse diameter; the cells of the perichondrium differentiated into osteoblasts instead of chondroblasts. Autoradiographic and histological findings suggested that the inability of the cartilage to increase in transverse diameter was related to the decreased rate of proliferative activity in the cell columns and not to the cessation of perichondrial chondrogenesis. On the basis of these findings two conclusions can be made. 1. Extrinsic mechanical stresses associated with function appear to be necessary for the normal interstitial growth of epiphyseal cartilage during postnatal development, suggesting that functional activity can influence the rate of cell proliferation. 2. Functional activity provides the stimulus for the differentiation of perichondrial cells into chondroblasts."}
{"id": "PMID:1098524", "title": "A Double-blind crossover trial of pseudoephedrine and triprolidine, alone and in combination, for the treatment of allergenic rhinitis.", "content": "In a double-blind crossover trial of pseudoephedrine 60 mg and triprolidine 2.5 mg, alone and in combination, on 40 volunteers suffering from allergic rhinitis, both drugs were found superior to placebo in reducing the effects of allergic rhinitis and were of equal efficacy. The combination tablet was consistently better than either drug in several of the assessments and was the treatment which the subjects, as a whole, preferred. Side effects were not a problem with any of the medications.", "contents": "A Double-blind crossover trial of pseudoephedrine and triprolidine, alone and in combination, for the treatment of allergenic rhinitis. In a double-blind crossover trial of pseudoephedrine 60 mg and triprolidine 2.5 mg, alone and in combination, on 40 volunteers suffering from allergic rhinitis, both drugs were found superior to placebo in reducing the effects of allergic rhinitis and were of equal efficacy. The combination tablet was consistently better than either drug in several of the assessments and was the treatment which the subjects, as a whole, preferred. Side effects were not a problem with any of the medications."}
{"id": "PMID:1098526", "title": "Exercise-induced bronchospasm: evaluation of albuterol aerosol.", "content": "Albuterol aerosol was compared with isoproterenol and placebo in a double-blind, cross-over study in 12 asthmatic children. A significant increase in mean peak expiratory flow rate 15 minutes after albuterol inhalation persisted for at least five hours after treatment. Inhalation of albuterol one hour before treadmill exercise inhibited exercise-induced bronchospasm.", "contents": "Exercise-induced bronchospasm: evaluation of albuterol aerosol. Albuterol aerosol was compared with isoproterenol and placebo in a double-blind, cross-over study in 12 asthmatic children. A significant increase in mean peak expiratory flow rate 15 minutes after albuterol inhalation persisted for at least five hours after treatment. Inhalation of albuterol one hour before treadmill exercise inhibited exercise-induced bronchospasm."}
{"id": "PMID:1098527", "title": "Specificity of an immunofluorescent test for identification of type specific ragweed antibodies.", "content": "An immunofluorescent test using ragweed pollen grains for identification of immunolglobulin type-specific rag weed antibodies is described.  The test measured effectively antibody specificity produced by immunotherapy with Allpyral.  Repeating the test after ragweed inhibition and the use of an unrelated pollen grain verified the specificity of the test.", "contents": "Specificity of an immunofluorescent test for identification of type specific ragweed antibodies. An immunofluorescent test using ragweed pollen grains for identification of immunolglobulin type-specific rag weed antibodies is described.  The test measured effectively antibody specificity produced by immunotherapy with Allpyral.  Repeating the test after ragweed inhibition and the use of an unrelated pollen grain verified the specificity of the test."}
{"id": "PMID:1098528", "title": "Development of specific lymphocyte immunostimulation and tuberculin skin reactivity in swine infected with Mycobacterium bovis and Mycobacterium avium.", "content": "Swine infected with Mycobacterium bovis and Mycobacterium avium were studied for development and specificity of lymphocyte immunostimulation and tuberculin skin reactivity to purified protein derivatives (PPD) of tuberculins. Lymphocytes from infected animals had maximal blastogenic responses to PPD prepared from the homologous organisms. The time of appearance of specifically sensitized lymphocytes was studied, as was the specificity of skin reactivity. In vitro lymphocyte responses of M avium serotype 1-infected swine to M avium serotype 1 PPD were greater than were the responses of M avium serotype 2-infected swine to the same antigen. Results indicate that the specific stimulation of sensitized lymphocytes might prove useful in the diagnosis of tuberculosis in swine and in differentiating infections due to different serotypes of M avium.", "contents": "Development of specific lymphocyte immunostimulation and tuberculin skin reactivity in swine infected with Mycobacterium bovis and Mycobacterium avium. Swine infected with Mycobacterium bovis and Mycobacterium avium were studied for development and specificity of lymphocyte immunostimulation and tuberculin skin reactivity to purified protein derivatives (PPD) of tuberculins. Lymphocytes from infected animals had maximal blastogenic responses to PPD prepared from the homologous organisms. The time of appearance of specifically sensitized lymphocytes was studied, as was the specificity of skin reactivity. In vitro lymphocyte responses of M avium serotype 1-infected swine to M avium serotype 1 PPD were greater than were the responses of M avium serotype 2-infected swine to the same antigen. Results indicate that the specific stimulation of sensitized lymphocytes might prove useful in the diagnosis of tuberculosis in swine and in differentiating infections due to different serotypes of M avium."}
{"id": "PMID:1098529", "title": "Pathogenesis of in utero infection: experimental infection of five-week-old porcine fetuses with porcine parvovirus.", "content": "Injection of porcine parvovirus (PPV) into the allantoic fluid of 3 or 4 fetuses of each of 4 litters of PPV-immune gilts early in gestation (34 to 36 days) resulted in fetal maceration and mummification. A high concentration of virus demonstrated in many tissues of fetuses collected 1 week after initial intraallantoic exposure indicated extensive viral replication. Progressively lesser amounts of virus were isolated from tissues of macerated and mummified fetuses collected after longer intervals; however, direct examination of many of the same tissues by immunofluorescence microscopy revealed masses of viral antigen throughout. Abortion was not a sequela of infection of fetuses of immune gilts nor was either virus or viral antigen ever demonstrated in maternal tissues, including those contiguous with infected fetuses. Intrauterine transmission of PPV from fetuses exposed by intraallantoic injection of virus was only confirmed for 2 of the 18 littermate controls that were tested.", "contents": "Pathogenesis of in utero infection: experimental infection of five-week-old porcine fetuses with porcine parvovirus. Injection of porcine parvovirus (PPV) into the allantoic fluid of 3 or 4 fetuses of each of 4 litters of PPV-immune gilts early in gestation (34 to 36 days) resulted in fetal maceration and mummification. A high concentration of virus demonstrated in many tissues of fetuses collected 1 week after initial intraallantoic exposure indicated extensive viral replication. Progressively lesser amounts of virus were isolated from tissues of macerated and mummified fetuses collected after longer intervals; however, direct examination of many of the same tissues by immunofluorescence microscopy revealed masses of viral antigen throughout. Abortion was not a sequela of infection of fetuses of immune gilts nor was either virus or viral antigen ever demonstrated in maternal tissues, including those contiguous with infected fetuses. Intrauterine transmission of PPV from fetuses exposed by intraallantoic injection of virus was only confirmed for 2 of the 18 littermate controls that were tested."}
{"id": "PMID:1098530", "title": "Experimentally induced infection of neonatal swine with porcine parvovirus.", "content": "One-day-old pigs were exposed to porcine parvovirus (PPV) and killed at postinoculation day (PID) 3 or 7. Signs of illness and lesions were not seen. Hemagglutination-inhibition (HI) antibodies for PPV were present in the serums of pigs killed on PID 7. As detected by viral isolation and immunofluorescence, PPV was mainly located in rapidly proliferating tissues. Heaviest concentration of viral antigen was in the germinal centers of lymph nodes and lightest concentration of antigen was in the central nervous system.", "contents": "Experimentally induced infection of neonatal swine with porcine parvovirus. One-day-old pigs were exposed to porcine parvovirus (PPV) and killed at postinoculation day (PID) 3 or 7. Signs of illness and lesions were not seen. Hemagglutination-inhibition (HI) antibodies for PPV were present in the serums of pigs killed on PID 7. As detected by viral isolation and immunofluorescence, PPV was mainly located in rapidly proliferating tissues. Heaviest concentration of viral antigen was in the germinal centers of lymph nodes and lightest concentration of antigen was in the central nervous system."}
{"id": "PMID:1098531", "title": "Factors involved in bactericidal activity of sheep serum.", "content": "The bactericidal activity of blood, plasma, and serum from sheep was measured with 3 strains of Escherichia coli. Plasma showed greater bactericidal activity than blood or serum for strains O78:K80(B) and Lilly. The antibody-complement system of normal serum killed the rough strain, Lilly, but neither antibody nor complement alone were bactericidal. Antibody, complement, and an additional heat-stable cofactor were necessary for killing strain O78:K80(B). Strain 3662 was not susceptible to the lethal action of the antibody-complement system. Capsular and flagellar antibodies did not appear to play any part in the bactericidal activity of serum. There was no relationship between the titer of antibodies to the somatic antigen and the bactericidal titer in the immune serums. The antibodies necessary for killing the smooth strain of E coli, O78:K80(B), were absorbed with the rough strain, Lilly, but not by the other smooth strain, 3662. It is suggested that the target antigen of the bactericidal antibody is not the somatic antigen, but a rough antigen internal to the O polysaccharide.", "contents": "Factors involved in bactericidal activity of sheep serum. The bactericidal activity of blood, plasma, and serum from sheep was measured with 3 strains of Escherichia coli. Plasma showed greater bactericidal activity than blood or serum for strains O78:K80(B) and Lilly. The antibody-complement system of normal serum killed the rough strain, Lilly, but neither antibody nor complement alone were bactericidal. Antibody, complement, and an additional heat-stable cofactor were necessary for killing strain O78:K80(B). Strain 3662 was not susceptible to the lethal action of the antibody-complement system. Capsular and flagellar antibodies did not appear to play any part in the bactericidal activity of serum. There was no relationship between the titer of antibodies to the somatic antigen and the bactericidal titer in the immune serums. The antibodies necessary for killing the smooth strain of E coli, O78:K80(B), were absorbed with the rough strain, Lilly, but not by the other smooth strain, 3662. It is suggested that the target antigen of the bactericidal antibody is not the somatic antigen, but a rough antigen internal to the O polysaccharide."}
{"id": "PMID:1098532", "title": "Bactericidal and opsonic activities of normal sheep serum against gram-negative bacteria.", "content": "The bactericidal and opsonic activities of normal sheep serum against 9 smooth and 4 rough strains of gram-negative bacteria were measured. Three smooth strains-Escherichia coli 3662, Salmonella typhimurium, and Salmonella gallinarum-were resistant to the bactericidal action of normal sheep serum with or without complement. Six smooth strains-E coli O78:K80(B), Salmonella arizonae, Proteus inconstans, Klebsiella pneumoniae, Salmonella stanley, and Salmonella abortus-equi-were moderately sensitive to bactericidal activity of fresh sheep serum. After complement was added, serum heated at 56 C for 30 minutes did not regain its bactericidal activity against E coli O78:K80(B), P inconstans, nor S abortus-equi. Four rough strains-E coli Lilly, Salmonella cholerae-suis, Shigella sonnei, and Hafnia alvei-were very sensitive to the bactericidal activity of normal sheep serum. Somatic agglutinins were demonstrated in all of the sheep serums against all of the bacteria, but this antibody activity was partially destroyed by heating at 56 C for 30 minutes. The opsonic activity of normal sheep serum was demonstrated in normal mice. In the mouse peritoneal cavity, normal sheep serum enhanced opsonic activity against all smooth strains and 1 rough strain of the bacteria. The other 3 rough strains were extremely sensitive to the opsonic system of normal mice.", "contents": "Bactericidal and opsonic activities of normal sheep serum against gram-negative bacteria. The bactericidal and opsonic activities of normal sheep serum against 9 smooth and 4 rough strains of gram-negative bacteria were measured. Three smooth strains-Escherichia coli 3662, Salmonella typhimurium, and Salmonella gallinarum-were resistant to the bactericidal action of normal sheep serum with or without complement. Six smooth strains-E coli O78:K80(B), Salmonella arizonae, Proteus inconstans, Klebsiella pneumoniae, Salmonella stanley, and Salmonella abortus-equi-were moderately sensitive to bactericidal activity of fresh sheep serum. After complement was added, serum heated at 56 C for 30 minutes did not regain its bactericidal activity against E coli O78:K80(B), P inconstans, nor S abortus-equi. Four rough strains-E coli Lilly, Salmonella cholerae-suis, Shigella sonnei, and Hafnia alvei-were very sensitive to the bactericidal activity of normal sheep serum. Somatic agglutinins were demonstrated in all of the sheep serums against all of the bacteria, but this antibody activity was partially destroyed by heating at 56 C for 30 minutes. The opsonic activity of normal sheep serum was demonstrated in normal mice. In the mouse peritoneal cavity, normal sheep serum enhanced opsonic activity against all smooth strains and 1 rough strain of the bacteria. The other 3 rough strains were extremely sensitive to the opsonic system of normal mice."}
{"id": "PMID:1098534", "title": "Point source epidemic of Mycoplasma pneumoniae infection in a prosthodontics laboratory.", "content": "A cluster of 10 cases of Mycoplasma pneumoniae infection involving persons working in a prosthodontics laboratory is descrbied.  The pattern indicates a point source epidemic.  The suspected mode of spread was a fine-particle aerosol generated from abrasive drilling of contaminated false teeth.", "contents": "Point source epidemic of Mycoplasma pneumoniae infection in a prosthodontics laboratory. A cluster of 10 cases of Mycoplasma pneumoniae infection involving persons working in a prosthodontics laboratory is descrbied.  The pattern indicates a point source epidemic.  The suspected mode of spread was a fine-particle aerosol generated from abrasive drilling of contaminated false teeth."}
{"id": "PMID:1098538", "title": "[Surgery of vesicoureteral reflux (author's transl)].", "content": "The objective of surgery of vesicureteral reflux is to block the way of urine from urinary bladder to the ureter without upsetting normal flux of urine in descending sense. Most important techniques used are described from the beginning of this surgery until nowadays and specially the Leadbetter-Politano technique, which accomplished most essential requirements for the obtention of favourable results. Finally special considerations are enumerated to keep in mind in treatment of reflux when is associated to other malformations of urinary tract like the ureteral diverticule of double ureter.", "contents": "[Surgery of vesicoureteral reflux (author's transl)]. The objective of surgery of vesicureteral reflux is to block the way of urine from urinary bladder to the ureter without upsetting normal flux of urine in descending sense. Most important techniques used are described from the beginning of this surgery until nowadays and specially the Leadbetter-Politano technique, which accomplished most essential requirements for the obtention of favourable results. Finally special considerations are enumerated to keep in mind in treatment of reflux when is associated to other malformations of urinary tract like the ureteral diverticule of double ureter."}
{"id": "PMID:1098539", "title": "[Urinary tract infections presenting with jaundice (author's transl)].", "content": "Ten two to ten weeks old children are presented with urinary tract infection and jaundice. In six cases \"E. coli\" was the causative organism. Laboratory data seemed to indicate that hemolisis was an important factor in the pathogenesis of jaundice. Treatment with appropriate antibiotics was favorable in all cases. Urinary tract infection with bacteriemia and jaundice is probably the most frequent cause of jaundice among children of two to ten weeks of age.", "contents": "[Urinary tract infections presenting with jaundice (author's transl)]. Ten two to ten weeks old children are presented with urinary tract infection and jaundice. In six cases \"E. coli\" was the causative organism. Laboratory data seemed to indicate that hemolisis was an important factor in the pathogenesis of jaundice. Treatment with appropriate antibiotics was favorable in all cases. Urinary tract infection with bacteriemia and jaundice is probably the most frequent cause of jaundice among children of two to ten weeks of age."}
{"id": "PMID:1098536", "title": "[Intestinal recirculation in the management of experimental short bowel syndrome (author's transl)].", "content": "A short bowel syndrome was produced in 33 mongrel puppies less than one month of age, by resecting 80% of the small bowel. An end-to-end anastomosis was performed in 14 dogs (Group A). Sixteen dogs were reanastomosed by performing an isoperistaltic circular intestinal loop, modified from Tanner and Mackby (Group B). Three dogs had a circular loop performed using Mackby method, but this technique was abandoned because of intraluminal stasis. Long term survival on 18 dogs was 12,5% for Group A and 57% for Group B. Weight gain on the survivors was over 25% higher in Group B. Intestinal transit time was abnormally short only in Group A. The loop procedure seems worthy of further clinical trial.", "contents": "[Intestinal recirculation in the management of experimental short bowel syndrome (author's transl)]. A short bowel syndrome was produced in 33 mongrel puppies less than one month of age, by resecting 80% of the small bowel. An end-to-end anastomosis was performed in 14 dogs (Group A). Sixteen dogs were reanastomosed by performing an isoperistaltic circular intestinal loop, modified from Tanner and Mackby (Group B). Three dogs had a circular loop performed using Mackby method, but this technique was abandoned because of intraluminal stasis. Long term survival on 18 dogs was 12,5% for Group A and 57% for Group B. Weight gain on the survivors was over 25% higher in Group B. Intestinal transit time was abnormally short only in Group A. The loop procedure seems worthy of further clinical trial."}
{"id": "PMID:1098537", "title": "[Rickets and tetany in the Spanish bibliography of the ninetenth century (author's transl)].", "content": "An historical study of the relationship between rickets and tetany through publications edited in Spain from 1800 to 1914 is presented. Twelve collections of journals, both pediatric and general medical, and likewise Medical Treatises, were used. In spite of the recognition of nervous phenomena accompanying rickets towards the beginning of the century, the clear relationship between rickets and tetany was not recognised until the beginning of this century. The causes of this delay are discussed, the main one being the limitations of an anatomoclinic mentality. The limited contribution and the low level of information of the Spanish author is emphasized.", "contents": "[Rickets and tetany in the Spanish bibliography of the ninetenth century (author's transl)]. An historical study of the relationship between rickets and tetany through publications edited in Spain from 1800 to 1914 is presented. Twelve collections of journals, both pediatric and general medical, and likewise Medical Treatises, were used. In spite of the recognition of nervous phenomena accompanying rickets towards the beginning of the century, the clear relationship between rickets and tetany was not recognised until the beginning of this century. The causes of this delay are discussed, the main one being the limitations of an anatomoclinic mentality. The limited contribution and the low level of information of the Spanish author is emphasized."}
{"id": "PMID:1098541", "title": "[Antigenic relationships between \"Levinea amalonatica\" and \"Shigella dysenteriae\" and \"boydii\" (author's transl)].", "content": "Twenty-eight strains among thirty-five Levinea amalonatica show O antigenic relationships with Shigella as follows: with S. dysenteriae 3 (5 strains), S. dysenteriae 4 (1 strain), S. dysenteriae 5 (3 strains), S. dysenteriae 7 (11 strains), S. boydii 6 (7 strains), and with both S. boydii 15 and S. dysenteriae 8 (1 strain). These strains do not induce purulent keratogenous conjunctivitis in Guinea pigs as Shigella do.", "contents": "[Antigenic relationships between \"Levinea amalonatica\" and \"Shigella dysenteriae\" and \"boydii\" (author's transl)]. Twenty-eight strains among thirty-five Levinea amalonatica show O antigenic relationships with Shigella as follows: with S. dysenteriae 3 (5 strains), S. dysenteriae 4 (1 strain), S. dysenteriae 5 (3 strains), S. dysenteriae 7 (11 strains), S. boydii 6 (7 strains), and with both S. boydii 15 and S. dysenteriae 8 (1 strain). These strains do not induce purulent keratogenous conjunctivitis in Guinea pigs as Shigella do."}
{"id": "PMID:1098542", "title": "[A comparison of the serological effects of classical cholera vaccine and of purified fraction vaccine, with or without simultaneous yellow fever vaccine (author's transl)].", "content": "In order to test whether simultaneously administered cholera vaccine has a depressive effect on yellow fever vaccine, a controlled trial was undertaken on school-age children in the South-Central Province of Cameroun. In addition to this principle objective, the study also permitted a comparison of the serological response in subjects vaccinated with classical cholera vaccine and in those vaccinated with a purified fraction vaccine, either with or without simultaneous yellow fever vaccine. The evaluation was measured by changes in vibriocidal antibodies and cholera agglutinins 30 days after vaccination. Only subjects without cholera antibodies prior to the study, were included. 1) Results obtained by assay of vibriocidal antibodies. It was confirmed that, no matter which cholera vaccine was used, the simultaneous administration of yellow fever vaccine had no influence on the percentage of subjects showing a significant rise in vibriocidal antibodies (4-fold increase in titre) following vaccination. In addition, in this study the purified fraction vaccine resulted in a significantly higher rate of seroconversion than did the classical vaccine. However, in comparison to other studies using classical cholera vaccine, our figures for seroconversion after purified fraction vaccine show very little, if any, differences. 2) Results obtained by assay of agglutinating antibodies. When measured by this method, there was a high frequency of non-reactors to the vaccines. This may be attributed to the date of the post vaccination blood speciment (30th day after vaccination). It has been shown that agglutinins decay rapidly after the 15th day following clinical cholera. Thus, the late date of the second speciment after vaccination could explain why we were unable to show any difference in the level of agglutinin after either classical or purified cholera vaccination. The simultaneous administration of the yellow fever vaccine did not influence the titre of agglutinins induced by the classic cholera vaccine. On the other hand, using the association, the seroconversion rate as observed on the 30th day post vaccination was significantly higher than that observed when the fraction was administered alone. If one accepts the generally admitted specificity of the agglutination reaction after clinical disease, two hypotheses can be considered: a) the yellow fever vaccine has an adjuvant effect for the production of antibodies induced by the purified fraction vaccine, or b) the addition of yellow fever vaccine has a retarding effect on the elimination of the agglutinins which, in the natural disease, are rapidly eliminated. Further studies to verify these hypothesis should be undertaken.", "contents": "[A comparison of the serological effects of classical cholera vaccine and of purified fraction vaccine, with or without simultaneous yellow fever vaccine (author's transl)]. In order to test whether simultaneously administered cholera vaccine has a depressive effect on yellow fever vaccine, a controlled trial was undertaken on school-age children in the South-Central Province of Cameroun. In addition to this principle objective, the study also permitted a comparison of the serological response in subjects vaccinated with classical cholera vaccine and in those vaccinated with a purified fraction vaccine, either with or without simultaneous yellow fever vaccine. The evaluation was measured by changes in vibriocidal antibodies and cholera agglutinins 30 days after vaccination. Only subjects without cholera antibodies prior to the study, were included. 1) Results obtained by assay of vibriocidal antibodies. It was confirmed that, no matter which cholera vaccine was used, the simultaneous administration of yellow fever vaccine had no influence on the percentage of subjects showing a significant rise in vibriocidal antibodies (4-fold increase in titre) following vaccination. In addition, in this study the purified fraction vaccine resulted in a significantly higher rate of seroconversion than did the classical vaccine. However, in comparison to other studies using classical cholera vaccine, our figures for seroconversion after purified fraction vaccine show very little, if any, differences. 2) Results obtained by assay of agglutinating antibodies. When measured by this method, there was a high frequency of non-reactors to the vaccines. This may be attributed to the date of the post vaccination blood speciment (30th day after vaccination). It has been shown that agglutinins decay rapidly after the 15th day following clinical cholera. Thus, the late date of the second speciment after vaccination could explain why we were unable to show any difference in the level of agglutinin after either classical or purified cholera vaccination. The simultaneous administration of the yellow fever vaccine did not influence the titre of agglutinins induced by the classic cholera vaccine. On the other hand, using the association, the seroconversion rate as observed on the 30th day post vaccination was significantly higher than that observed when the fraction was administered alone. If one accepts the generally admitted specificity of the agglutination reaction after clinical disease, two hypotheses can be considered: a) the yellow fever vaccine has an adjuvant effect for the production of antibodies induced by the purified fraction vaccine, or b) the addition of yellow fever vaccine has a retarding effect on the elimination of the agglutinins which, in the natural disease, are rapidly eliminated. Further studies to verify these hypothesis should be undertaken."}
{"id": "PMID:1098549", "title": "EGG analysis: a history and a prospectus.", "content": "Progress in EGGy has been largely limited to those clinical situations in which there are pragmatic associations between observed electrical activity and disease processes. Lack of understanding of the functional significances of EEG patterns continues to present the major challenge to those who would use computational methods to increase our understanding of mental processes.", "contents": "EGG analysis: a history and a prospectus. Progress in EGGy has been largely limited to those clinical situations in which there are pragmatic associations between observed electrical activity and disease processes. Lack of understanding of the functional significances of EEG patterns continues to present the major challenge to those who would use computational methods to increase our understanding of mental processes."}
{"id": "PMID:1098543", "title": "Experimental aural cholesteatoma causing bone resorption.", "content": "A series of experiments were carried out on 55 guinea pigs in four groups to study the conditions fostering bone resorbin epidermal cysts. The first group had free grafts of canal wall skin applied to the cochlea with and without talc application. The second group had canal skin flaps applied to the cochlea with and without subsequent talc application. The third group had talc applied either to the tympanic membrane or on the cochlea. The fourth group had a canal skin flap inserted into a mucosal pocket in the bulla. The animals were killed three to four months after surgery and the temporal bones were prepared for histology. Epidermal cysts were found at the cochlea in 8 of 55 animals. Cochlear fistulas were found in 6 of 55 animals. The fistulas were associated with epidermal cysts in three cases, otitis media in two cases, and talc granuloma in one case. These experiments show that migrating skin attached to a source of epithelium is capable of inducing bone resorption. Chronic foreign body granulomas and chronic sepsis are also capable of resorbing bone. These three conditions all produce a layer of undifferentiated connective tissue containing chronic inflammatory cells lying against the resorbing bone.", "contents": "Experimental aural cholesteatoma causing bone resorption. A series of experiments were carried out on 55 guinea pigs in four groups to study the conditions fostering bone resorbin epidermal cysts. The first group had free grafts of canal wall skin applied to the cochlea with and without talc application. The second group had canal skin flaps applied to the cochlea with and without subsequent talc application. The third group had talc applied either to the tympanic membrane or on the cochlea. The fourth group had a canal skin flap inserted into a mucosal pocket in the bulla. The animals were killed three to four months after surgery and the temporal bones were prepared for histology. Epidermal cysts were found at the cochlea in 8 of 55 animals. Cochlear fistulas were found in 6 of 55 animals. The fistulas were associated with epidermal cysts in three cases, otitis media in two cases, and talc granuloma in one case. These experiments show that migrating skin attached to a source of epithelium is capable of inducing bone resorption. Chronic foreign body granulomas and chronic sepsis are also capable of resorbing bone. These three conditions all produce a layer of undifferentiated connective tissue containing chronic inflammatory cells lying against the resorbing bone."}
{"id": "PMID:1098544", "title": "Grafting materials and methods in reconstructive ear surgery.", "content": "The evolution of tympanic membrane grafting over the past 20 years has been based on biologic tissues of mesodermal origin which contain a collagen matrix. The dominant theme of this evolution has been the modification of these collagen membranes by physical and chemical agents to achieve a more ideal graft. The graft material and the operation should be suited to specific problems. Current operative techniques and graft materials are discussed. It is believed that there will be continued modification and manipulation of collagen in the future to achieve a more ideal graft for tympanic membrane restoration.", "contents": "Grafting materials and methods in reconstructive ear surgery. The evolution of tympanic membrane grafting over the past 20 years has been based on biologic tissues of mesodermal origin which contain a collagen matrix. The dominant theme of this evolution has been the modification of these collagen membranes by physical and chemical agents to achieve a more ideal graft. The graft material and the operation should be suited to specific problems. Current operative techniques and graft materials are discussed. It is believed that there will be continued modification and manipulation of collagen in the future to achieve a more ideal graft for tympanic membrane restoration."}
{"id": "PMID:1098545", "title": "Reconstruction of the open mastoidectomy ear: a six year progress report.", "content": "This report deals with 61 mastoid cavities which were revised or reconstructed using homograft techniques over a six-year period. Discussed are the variety of homograft procurement techniques and surgical homograft techniques which have been abandoned and included in a review of 16 consecutive mastoid revisions performed from 1973-1974 utilizing a standard surgical technique.", "contents": "Reconstruction of the open mastoidectomy ear: a six year progress report. This report deals with 61 mastoid cavities which were revised or reconstructed using homograft techniques over a six-year period. Discussed are the variety of homograft procurement techniques and surgical homograft techniques which have been abandoned and included in a review of 16 consecutive mastoid revisions performed from 1973-1974 utilizing a standard surgical technique."}
{"id": "PMID:1098551", "title": "Chemotaxis in bacteria.", "content": "Bacteria swim by rotating their flagella. They alter course by abruptly changing the direction of this rotation. The probability of the occurrence of this event is biased by chemoreception. The bias depends on the way in which the concentration of the attractant or repellent changes with time. Sugars are detected as they bind to specific proteins which also play a role in transport. The way in which the receptors are coupled to the flagella is not known. The coupling may involve changes in membrane potential.", "contents": "Chemotaxis in bacteria. Bacteria swim by rotating their flagella. They alter course by abruptly changing the direction of this rotation. The probability of the occurrence of this event is biased by chemoreception. The bias depends on the way in which the concentration of the attractant or repellent changes with time. Sugars are detected as they bind to specific proteins which also play a role in transport. The way in which the receptors are coupled to the flagella is not known. The coupling may involve changes in membrane potential."}
{"id": "PMID:1098568", "title": "On the evolution of the physiological model.", "content": "Most of us who have concerned ourselves with models can perceive outlines like those above to catalog the future evolution of the expository function of models. In the context of a single class of computerized mathematical models of respiratory physiology, we can observe at once the burgeoning interest among scientists, and the similarities between model activity and the general organization of scientific information for use. Although physiological models have become quite advanced in their subject control, there is relatively little coordinated activity in the mechanization of the purposes and philosophical potential of automata. The outlines, however, are visible. An assiduous pursuit of the notion of \"explanation\" by machine is a major evolutionary step next to occur. It appears to us that various diagrams similar to Figures 5 or 6 can be created and investigated in terms of their relation to the human mind and in terms of formalizing rules for traversing from one plane to the next. The evolution of models will require program-making programs which can decide when and how to aggregate for deductive inference, and how far to penetrate top-down for explanation. The rules for identifying \"second order\" effects must be established. The decision to ignore or use these rules will be crucial. These are the means whereby the systems are traversed from plane to plane. In a word, models need to synthesize the means to ignore, \"forget,\" and gloss over; only then will we have useful tools for taking informed action in physiology, diagnosis in medicine, or the writing of \"scholarly\" reviews.", "contents": "On the evolution of the physiological model. Most of us who have concerned ourselves with models can perceive outlines like those above to catalog the future evolution of the expository function of models. In the context of a single class of computerized mathematical models of respiratory physiology, we can observe at once the burgeoning interest among scientists, and the similarities between model activity and the general organization of scientific information for use. Although physiological models have become quite advanced in their subject control, there is relatively little coordinated activity in the mechanization of the purposes and philosophical potential of automata. The outlines, however, are visible. An assiduous pursuit of the notion of \"explanation\" by machine is a major evolutionary step next to occur. It appears to us that various diagrams similar to Figures 5 or 6 can be created and investigated in terms of their relation to the human mind and in terms of formalizing rules for traversing from one plane to the next. The evolution of models will require program-making programs which can decide when and how to aggregate for deductive inference, and how far to penetrate top-down for explanation. The rules for identifying \"second order\" effects must be established. The decision to ignore or use these rules will be crucial. These are the means whereby the systems are traversed from plane to plane. In a word, models need to synthesize the means to ignore, \"forget,\" and gloss over; only then will we have useful tools for taking informed action in physiology, diagnosis in medicine, or the writing of \"scholarly\" reviews."}
{"id": "PMID:1098569", "title": "Interrelation between guanosine tetraphosphate accumulation, ribonucleic acid synthesis, and streptomycin lethality in Escherichia coli CP78.", "content": "The effects of streptomycin on the synthesis of ribonucleic acid (RNA) and guanosine-3'-diphosphate-5'-diphosphate have been examined in the isogenic strains CP78 (rel(+)) and CP79 (rel-). In the absence of the required amino acids arginine, leucine, or threonine, streptomycin stimulates RNA synthesis in CP78 and this stimulation coincides with cell death. However, in the absence of histidine, also a required amino acid, streptomycin kills the cells without stimulation of RNA synthesis above that which occurs in the absence of streptomycin. In all of these instances, guanosine-3'-diphosphate-5'-diphosphate levels vary inversely with RNA synthesis, decreasing when a stimulation of RNA synthesis occurs. Streptomycin has little effect on guanosine-3'-diphosphate-5'-diphosphate levels in CP78 in the absence of histidine. Another histidine auxotroph, 15 T(-)H(-)U(-) (rel(+)), does show streptomycin-stimulated synthesis of RNA which is coincident with cell death. CP79 (rel(-)) is 10 times more susceptible to streptomycin than CP78 and streptomycin causes an inhibition of the relaxed synthesis of RNA. The greater susceptibility of CP79 may be due to a greater initial uptake of streptomycin by CP79.", "contents": "Interrelation between guanosine tetraphosphate accumulation, ribonucleic acid synthesis, and streptomycin lethality in Escherichia coli CP78. The effects of streptomycin on the synthesis of ribonucleic acid (RNA) and guanosine-3'-diphosphate-5'-diphosphate have been examined in the isogenic strains CP78 (rel(+)) and CP79 (rel-). In the absence of the required amino acids arginine, leucine, or threonine, streptomycin stimulates RNA synthesis in CP78 and this stimulation coincides with cell death. However, in the absence of histidine, also a required amino acid, streptomycin kills the cells without stimulation of RNA synthesis above that which occurs in the absence of streptomycin. In all of these instances, guanosine-3'-diphosphate-5'-diphosphate levels vary inversely with RNA synthesis, decreasing when a stimulation of RNA synthesis occurs. Streptomycin has little effect on guanosine-3'-diphosphate-5'-diphosphate levels in CP78 in the absence of histidine. Another histidine auxotroph, 15 T(-)H(-)U(-) (rel(+)), does show streptomycin-stimulated synthesis of RNA which is coincident with cell death. CP79 (rel(-)) is 10 times more susceptible to streptomycin than CP78 and streptomycin causes an inhibition of the relaxed synthesis of RNA. The greater susceptibility of CP79 may be due to a greater initial uptake of streptomycin by CP79."}
{"id": "PMID:1098570", "title": "Killing and lysis of Echerichia coli in the presence of choloramphenicol: relation to cellular magensim.", "content": "Treatment of Escherichia coli K-10 with 100 mug of chloramphenicol per ml for periods greater than 30 min leads to progressive lysis and killing of cells. The bactericidal action of the antibiotic is dependent on cell growth and physiology; only rapidly dividing cells are susceptible to killing; resting or slowly growing cells are not. The presence of excess Mg(2+) in the growth medium specifically and competitively prevents excretion of macromolecules and cell lysis. However, inhibition of protein synthesis and killing of cells still occur even in the presence of added Mg(2+). The possible relation of these effects to the mode of action of chloramphenicol is discussed.", "contents": "Killing and lysis of Echerichia coli in the presence of choloramphenicol: relation to cellular magensim. Treatment of Escherichia coli K-10 with 100 mug of chloramphenicol per ml for periods greater than 30 min leads to progressive lysis and killing of cells. The bactericidal action of the antibiotic is dependent on cell growth and physiology; only rapidly dividing cells are susceptible to killing; resting or slowly growing cells are not. The presence of excess Mg(2+) in the growth medium specifically and competitively prevents excretion of macromolecules and cell lysis. However, inhibition of protein synthesis and killing of cells still occur even in the presence of added Mg(2+). The possible relation of these effects to the mode of action of chloramphenicol is discussed."}
{"id": "PMID:1098571", "title": "Comparison of tetracycline and minocyclie transport in Escherichia Coli.", "content": "Differences between minocycline and tetracycline transport were demonstrated in an Escherichia coli strain with and without an R factor (R46) which confers moderate Tc resistance. Minocycline uptake was similar in R(+) and R(-) organisms, whereas tetracycline uptake was decreased in the R(+) as compared to the R(-) organism. Sodium azide had little effect on tetracycline uptake by either strain but minocycline uptake was completely inhibited by azide. p-Chloromercuribenzoate greatly decreased tetracycline uptake in both strains while minocycline uptake was markedly augmented. Both minocycline and tetracycline were effective inducers of decreased tetracycline transport, but only tetracycline had an effect on minocycline uptake. Mutual inhibition of uptake of one antibiotic by the other could not be demonstrated. These studies indicate different mechanisms of transport of minocycline and tetracycline in E. coli.", "contents": "Comparison of tetracycline and minocyclie transport in Escherichia Coli. Differences between minocycline and tetracycline transport were demonstrated in an Escherichia coli strain with and without an R factor (R46) which confers moderate Tc resistance. Minocycline uptake was similar in R(+) and R(-) organisms, whereas tetracycline uptake was decreased in the R(+) as compared to the R(-) organism. Sodium azide had little effect on tetracycline uptake by either strain but minocycline uptake was completely inhibited by azide. p-Chloromercuribenzoate greatly decreased tetracycline uptake in both strains while minocycline uptake was markedly augmented. Both minocycline and tetracycline were effective inducers of decreased tetracycline transport, but only tetracycline had an effect on minocycline uptake. Mutual inhibition of uptake of one antibiotic by the other could not be demonstrated. These studies indicate different mechanisms of transport of minocycline and tetracycline in E. coli."}
{"id": "PMID:1098572", "title": "Effects of cis-Dichlorudiammineplatinum (II) and related transition metal complexes on Escherichia Coli.", "content": "A number of transition metal complexes, including the cis and trans isomers of dichlorodiammineplatinum (II), six complexes of rhodium (I), two of iridium (I), and one of ruthenium (II) have been tested for their ability to induce lambda prophage, to produce filamentous growth of Escherichia coli, and to be selectively toxic for strains with defects in the deoxyribonucleic acid repair system. Dichlorotetrakis(dimethylsulfoxide)ruthenium II [RuCl(2) (DMSO)(4)] was strictly similar to cis-dichlorodiammineplatinum II [cis PtCl(2) (NH(3))(2)] in the test for lambda induction, filamentous growth production, and selective toxicity for a recA(-) strain. [Rh COD 1,10-phenanthroline](+) Cl(-), though more toxic for recA(-) than for rec(+)E. coli, was scarcely effective in the test for filamentous growth and did not induce prophage. None of the other tested compounds showed any similarity with cis-PtCl(2)(NH(3))(2). Due to the interesting results obtained with cis-PtCl(2)(NH(3))(2) as an antitumor agent, it seems reasonable to propose RuCl(2)(DMSO)(4) as a potential antitumor substance.", "contents": "Effects of cis-Dichlorudiammineplatinum (II) and related transition metal complexes on Escherichia Coli. A number of transition metal complexes, including the cis and trans isomers of dichlorodiammineplatinum (II), six complexes of rhodium (I), two of iridium (I), and one of ruthenium (II) have been tested for their ability to induce lambda prophage, to produce filamentous growth of Escherichia coli, and to be selectively toxic for strains with defects in the deoxyribonucleic acid repair system. Dichlorotetrakis(dimethylsulfoxide)ruthenium II [RuCl(2) (DMSO)(4)] was strictly similar to cis-dichlorodiammineplatinum II [cis PtCl(2) (NH(3))(2)] in the test for lambda induction, filamentous growth production, and selective toxicity for a recA(-) strain. [Rh COD 1,10-phenanthroline](+) Cl(-), though more toxic for recA(-) than for rec(+)E. coli, was scarcely effective in the test for filamentous growth and did not induce prophage. None of the other tested compounds showed any similarity with cis-PtCl(2)(NH(3))(2). Due to the interesting results obtained with cis-PtCl(2)(NH(3))(2) as an antitumor agent, it seems reasonable to propose RuCl(2)(DMSO)(4) as a potential antitumor substance."}
{"id": "PMID:1098573", "title": "Salmonella survival on pecans as influenced by processing and storage conditions.", "content": "Survival of Salmonella senftenberg 775W, S. anatum, and S. typhimurium during exposure to currently practiced, as well as abusive, pecan processing and storage conditions was studied. Thermal treatments normally carried out during the processing of pecans are inadequate to consistently destroy salmonellae in highly contaminated inshell nuts. Pecan nut packing tissue was toxic to salmonellae, thus affording some protection against high initial contamination and subsequent survival of the organisms. Examinations of inoculated inshell pecans stored at -18, -7, 5, and 21 C for up to 32 weeks revealed that the extent of survival was inversely correlated to the storage temperature. S. senftenberg 775W and S. anatum were not detectable on inshell nuts after 16 weeks of storage at 21 C. Little decrease in viable population of the three species was noted on inoculated pecan halves stored at -18, -7, and 5 C for 32 weeks. Due to organoleptic quality deterioration in pecan nutmeats at elevated temperatures, sterilization methods other than thermal treatment appear to be required for the elimination of viable salmonellae from pecan nuts.", "contents": "Salmonella survival on pecans as influenced by processing and storage conditions. Survival of Salmonella senftenberg 775W, S. anatum, and S. typhimurium during exposure to currently practiced, as well as abusive, pecan processing and storage conditions was studied. Thermal treatments normally carried out during the processing of pecans are inadequate to consistently destroy salmonellae in highly contaminated inshell nuts. Pecan nut packing tissue was toxic to salmonellae, thus affording some protection against high initial contamination and subsequent survival of the organisms. Examinations of inoculated inshell pecans stored at -18, -7, 5, and 21 C for up to 32 weeks revealed that the extent of survival was inversely correlated to the storage temperature. S. senftenberg 775W and S. anatum were not detectable on inshell nuts after 16 weeks of storage at 21 C. Little decrease in viable population of the three species was noted on inoculated pecan halves stored at -18, -7, and 5 C for 32 weeks. Due to organoleptic quality deterioration in pecan nutmeats at elevated temperatures, sterilization methods other than thermal treatment appear to be required for the elimination of viable salmonellae from pecan nuts."}
{"id": "PMID:1098574", "title": "Potential pathogens in the environment: cultural reactions and nucleic acid studies on Klebsiella pneumoniae from clinical and environmental sources.", "content": "The phenotypic and nucleic acid properties of Klebsiella pneumoniae have been studied on cultures obtained from six different habitats (humans, vegetables, seeds, trees, rivers, and pulp mills). The 19 cultural reactions of 107 isolates varied significantly only in tryptophanase activity and dulcitol fermentation. The percentage of guanine plus cytosine base composition of 41 isolates varied from 53.9 to 59.2%. The range of percentage of guanine plus cytosine base composition for environmental klebsiellas was broader than that for the cultures of human origin. The range of deoxyribonucleic acid relative reassociation (homology) to the human K. pneumoniae reference strain extended from 5% to 100% and the chromosome molecular weights ranged from 2,200 x 10(6) to 3,000 x 10(6). The species of K. pneumoniae is thus molecularly more heterogeneous than previously thought and most isolates of human, pulp mill, and river origin are genetically indistinguishable. The presence of K. pneumoniae therefore represents a deterioration of the microbiological quality of the environment and should be considered of public health significance. At the present time the health significance of the molecularly more divergent strains, primarily of vegetable and seed origin, their relationship to klebsiellas of human origin, or to other genera of the Enterobacteriaceae is unclear.", "contents": "Potential pathogens in the environment: cultural reactions and nucleic acid studies on Klebsiella pneumoniae from clinical and environmental sources. The phenotypic and nucleic acid properties of Klebsiella pneumoniae have been studied on cultures obtained from six different habitats (humans, vegetables, seeds, trees, rivers, and pulp mills). The 19 cultural reactions of 107 isolates varied significantly only in tryptophanase activity and dulcitol fermentation. The percentage of guanine plus cytosine base composition of 41 isolates varied from 53.9 to 59.2%. The range of percentage of guanine plus cytosine base composition for environmental klebsiellas was broader than that for the cultures of human origin. The range of deoxyribonucleic acid relative reassociation (homology) to the human K. pneumoniae reference strain extended from 5% to 100% and the chromosome molecular weights ranged from 2,200 x 10(6) to 3,000 x 10(6). The species of K. pneumoniae is thus molecularly more heterogeneous than previously thought and most isolates of human, pulp mill, and river origin are genetically indistinguishable. The presence of K. pneumoniae therefore represents a deterioration of the microbiological quality of the environment and should be considered of public health significance. At the present time the health significance of the molecularly more divergent strains, primarily of vegetable and seed origin, their relationship to klebsiellas of human origin, or to other genera of the Enterobacteriaceae is unclear."}
{"id": "PMID:1098575", "title": "Membrane filter procedure for enumerating the component genera of the coliform group in seawater.", "content": "A facile, quantitative, membrane filter procedure (mC) for defining the distribution of coliform populations in seawater according to the component genera was developed. The procedure, which utilizes a series of in situ substrate tests to obviate the picking of colonies for identification, also provides an estimate of the total coliform density. When pure cultures of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae were suspended in seawater and held at 4 C for 24 h, between 56 and 100% of the cells which grew on nutrient agar spread plates at 35 C could be recovered by the mC procedure. Confirmation as coliforms of typical colonies from natural samples was about 95%. Assay variability was found to be insignificant. The recovery of coliforms from marine waters by the mC procedure was comparable to those obtainable by current methods. Klebsiella was differentiated by the urease reaction and E. coli by its ability to form indole. The confirmation frequencies for colonies designated as Klebsiella and E. coli by the in situ tests approached 95% for the former and 98% for the latter.", "contents": "Membrane filter procedure for enumerating the component genera of the coliform group in seawater. A facile, quantitative, membrane filter procedure (mC) for defining the distribution of coliform populations in seawater according to the component genera was developed. The procedure, which utilizes a series of in situ substrate tests to obviate the picking of colonies for identification, also provides an estimate of the total coliform density. When pure cultures of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae were suspended in seawater and held at 4 C for 24 h, between 56 and 100% of the cells which grew on nutrient agar spread plates at 35 C could be recovered by the mC procedure. Confirmation as coliforms of typical colonies from natural samples was about 95%. Assay variability was found to be insignificant. The recovery of coliforms from marine waters by the mC procedure was comparable to those obtainable by current methods. Klebsiella was differentiated by the urease reaction and E. coli by its ability to form indole. The confirmation frequencies for colonies designated as Klebsiella and E. coli by the in situ tests approached 95% for the former and 98% for the latter."}
{"id": "PMID:1098578", "title": "Ambroise Par\u00e9 (1510-1590) and the evolution of the surgical instrumentarium.", "content": "Ambroise Par\u00e9 (1510-1590) has done pioneer work not only in surgery but also in the teaching of surgery. For hemostasis, he reintroduced vascular ligation instead of cautery. Moreover, he has improved the surgical instrumentarium. He devised the grooved sound for the operation of incarcerated hernias. He also developed a method for the treatment of the urinary fistulas which frequently occurred as the consequence of the surgical removal of bladder calculi. He raised the level of fracture treatment. It was he who introduced the word 'bistoury' for the surgical knife.", "contents": "Ambroise Par\u00e9 (1510-1590) and the evolution of the surgical instrumentarium. Ambroise Par\u00e9 (1510-1590) has done pioneer work not only in surgery but also in the teaching of surgery. For hemostasis, he reintroduced vascular ligation instead of cautery. Moreover, he has improved the surgical instrumentarium. He devised the grooved sound for the operation of incarcerated hernias. He also developed a method for the treatment of the urinary fistulas which frequently occurred as the consequence of the surgical removal of bladder calculi. He raised the level of fracture treatment. It was he who introduced the word 'bistoury' for the surgical knife."}
{"id": "PMID:1098579", "title": "Experience with the use of polyglycolic acid suture material (Dexon) in 500 surgical patients.", "content": "A highly concise review is presented of the experimental and clinical literature concerning polyglycolic acid suture material, marketed for clinical use for the first time in 1970. It is a synthetic, completely absorbable, non-toxic product, which not only offers a number of advantages over catgut, but may also be used where non-absorbable materials were formerly applied exclusively. Our favorable experiences in 500 patients, who were subjected to many different kinds of general surgical operations, are in agreement with those published in the literature. We have gained the impression that suture fistulization, which may lead to protracted morbidity after use of non-absorbable material, has decreased in incidence and severity after the use of PGA suture material. This is a definite advantage.", "contents": "Experience with the use of polyglycolic acid suture material (Dexon) in 500 surgical patients. A highly concise review is presented of the experimental and clinical literature concerning polyglycolic acid suture material, marketed for clinical use for the first time in 1970. It is a synthetic, completely absorbable, non-toxic product, which not only offers a number of advantages over catgut, but may also be used where non-absorbable materials were formerly applied exclusively. Our favorable experiences in 500 patients, who were subjected to many different kinds of general surgical operations, are in agreement with those published in the literature. We have gained the impression that suture fistulization, which may lead to protracted morbidity after use of non-absorbable material, has decreased in incidence and severity after the use of PGA suture material. This is a definite advantage."}
{"id": "PMID:1098580", "title": "Effect of low-dose heparin on incidence of postoperative thrombosis in orthopaedic patients.", "content": "In a double-blind study, using matched controls, thrombo-embolic prophylaxis with subcutaneous low-dose heparin was studied in 25 patients admitted for orthopaedic surgery. Deep vein thrombosis (DVT) was detected by clinical assessment and by the 125I-fibrinogen leg-scanning technique. As a control the T-1/2 of the 125I-fibrinogen was measured. Six of the 13 patients in the control group and one in the heparin-treated group developed deep vein thrombosis within the first eight postoperative days.", "contents": "Effect of low-dose heparin on incidence of postoperative thrombosis in orthopaedic patients. In a double-blind study, using matched controls, thrombo-embolic prophylaxis with subcutaneous low-dose heparin was studied in 25 patients admitted for orthopaedic surgery. Deep vein thrombosis (DVT) was detected by clinical assessment and by the 125I-fibrinogen leg-scanning technique. As a control the T-1/2 of the 125I-fibrinogen was measured. Six of the 13 patients in the control group and one in the heparin-treated group developed deep vein thrombosis within the first eight postoperative days."}
{"id": "PMID:1098581", "title": "Preliminary studies on the prolongation of the survival of mouse skin allografts cultured on a solid medium.", "content": "Skin allografts were transferred between individuals of white Swiss mice. No first set reaction was observed, but a second set reaction which resulted in accelerated rejection of the graft, could be induced by a second graft transplanted 2 weeks after the first. However, the histoincompatibility of the graft which causes the rejection, could be temporarily weakened when a graft had been cultured \"in vitro\" for at least 6 weeks prior to grafting. Nevertheless, histocompatibility differences between two different species e.g. man and mouse, could not be weakened at all by the tissue culture method.", "contents": "Preliminary studies on the prolongation of the survival of mouse skin allografts cultured on a solid medium. Skin allografts were transferred between individuals of white Swiss mice. No first set reaction was observed, but a second set reaction which resulted in accelerated rejection of the graft, could be induced by a second graft transplanted 2 weeks after the first. However, the histoincompatibility of the graft which causes the rejection, could be temporarily weakened when a graft had been cultured \"in vitro\" for at least 6 weeks prior to grafting. Nevertheless, histocompatibility differences between two different species e.g. man and mouse, could not be weakened at all by the tissue culture method."}
{"id": "PMID:1098582", "title": "Single daily dose of allopurinol.", "content": "Allopurinol was administered to seven patients with gout to compare the effects of three different methods of administration. Allopurinol 100 mg given three times daily. Allopurinol given once daily as three 100 mg tablets. Allopurinol given once daily as a single 300 mg tablet. Allopurinol as a single dose in the morning gave as sustained control of plasma levels as did divided administration.", "contents": "Single daily dose of allopurinol. Allopurinol was administered to seven patients with gout to compare the effects of three different methods of administration. Allopurinol 100 mg given three times daily. Allopurinol given once daily as three 100 mg tablets. Allopurinol given once daily as a single 300 mg tablet. Allopurinol as a single dose in the morning gave as sustained control of plasma levels as did divided administration."}
{"id": "PMID:1098583", "title": "The chemotherapy of rodent malaria, XXI. Action of quinine and WR 122 (a 9-phenanthrenemethanol) on the fine structure of Plasmodium berghei in mouse blood.", "content": "The effects of quinine and a 9-phenanthrenemethanol, WR 122,455 on the fine structure of Plasmodium berghei have been investigated. Changes consequent upon quinine treatment were noted in the outer membranes (including those that surround the \"food vacuole\") and the digestive vacuoles. These changes were followed by cytoplasmic degeneration and vacuolization. WR 122,455 caused morphological changes in at least three areas of the parasite, (1) the outer membranes, (2) the digestive vacuole and (3) the nucleus. The relationship of these changes to the mode of action of these compounds is discussed. It seems possible that they interfere primarily with limiting membrane-mitochondrial functions. The modifications of pigment formation are characteristic of these compounds and differ from those seen with chloroquine or primaquine.", "contents": "The chemotherapy of rodent malaria, XXI. Action of quinine and WR 122 (a 9-phenanthrenemethanol) on the fine structure of Plasmodium berghei in mouse blood. The effects of quinine and a 9-phenanthrenemethanol, WR 122,455 on the fine structure of Plasmodium berghei have been investigated. Changes consequent upon quinine treatment were noted in the outer membranes (including those that surround the \"food vacuole\") and the digestive vacuoles. These changes were followed by cytoplasmic degeneration and vacuolization. WR 122,455 caused morphological changes in at least three areas of the parasite, (1) the outer membranes, (2) the digestive vacuole and (3) the nucleus. The relationship of these changes to the mode of action of these compounds is discussed. It seems possible that they interfere primarily with limiting membrane-mitochondrial functions. The modifications of pigment formation are characteristic of these compounds and differ from those seen with chloroquine or primaquine."}
{"id": "PMID:1098584", "title": "The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of P. berghei in screening for blood schizontocidal activity.", "content": "Data are provided on the activity of a variety of antimalarial drugs against drug-sensitive and drug-resistant lines of Plasmodium berghei in albino mice. Parallel data for the response of the drug-sensitive parasites to these compounds in vitro indicate whether the drugs have a chloroquine-like or quinine-like type of action, or neither. The value of this test system for drug evaluation is debated. It is concluded that the in vivo tests do provide a valuable indication of the potential use of a compound against drug-resistant malaria parasites of man. The tests may also provide a good indication of the future value of new compounds against strains of parasites that are already resistant to chloroquine. Where necessary, steps should be taken to protect promising new compounds.", "contents": "The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of P. berghei in screening for blood schizontocidal activity. Data are provided on the activity of a variety of antimalarial drugs against drug-sensitive and drug-resistant lines of Plasmodium berghei in albino mice. Parallel data for the response of the drug-sensitive parasites to these compounds in vitro indicate whether the drugs have a chloroquine-like or quinine-like type of action, or neither. The value of this test system for drug evaluation is debated. It is concluded that the in vivo tests do provide a valuable indication of the potential use of a compound against drug-resistant malaria parasites of man. The tests may also provide a good indication of the future value of new compounds against strains of parasites that are already resistant to chloroquine. Where necessary, steps should be taken to protect promising new compounds."}
{"id": "PMID:1098585", "title": "Sudden increase in virulence in a strain of Plasmodium berghei yoelii.", "content": "The mild and chronic 17X strain of Plasmodium berghei yoelii showed a sudden increase in virulence following a period of 110 days in the deep freeze. The enhanced virulence was seen in a very high and early parasite peak in the blood and a 100% mortality of all infected mice. The exalted virulence remained unaltered following a number of blood transfers of the strain and after four cyclical transmissions through Anopheles stephensi. Enzyme pattern studies revealed that the virulent strain possessed the enzyme types GPI-1 and 6 PGD-4,-both characteristic for strain 17X of P.b. yoelii. Studies carried out to investigate the possibility of a concomitant viral infection transferred by blood passage, which could have been responsible for the enhanced virulence and mortality in the infected mice, showed no presence of any detectable virus in the blood of the P.b. yoelii infected mice. It is suggested that virulence may provide a marker in genetic work of rodent plasmodia and be included in association with enzyme and drug resistance markers.", "contents": "Sudden increase in virulence in a strain of Plasmodium berghei yoelii. The mild and chronic 17X strain of Plasmodium berghei yoelii showed a sudden increase in virulence following a period of 110 days in the deep freeze. The enhanced virulence was seen in a very high and early parasite peak in the blood and a 100% mortality of all infected mice. The exalted virulence remained unaltered following a number of blood transfers of the strain and after four cyclical transmissions through Anopheles stephensi. Enzyme pattern studies revealed that the virulent strain possessed the enzyme types GPI-1 and 6 PGD-4,-both characteristic for strain 17X of P.b. yoelii. Studies carried out to investigate the possibility of a concomitant viral infection transferred by blood passage, which could have been responsible for the enhanced virulence and mortality in the infected mice, showed no presence of any detectable virus in the blood of the P.b. yoelii infected mice. It is suggested that virulence may provide a marker in genetic work of rodent plasmodia and be included in association with enzyme and drug resistance markers."}
{"id": "PMID:1098586", "title": "Role of complement components in the susceptibility to Plasmodium berghei infection among inbred strains of mice.", "content": "Inbred strains of mice, sensitive and resistant to Plasmodium berghei infections, including a complement deficient strain were infected with P. berghei. Daily levels of parasitaemia were determined and serum levels of complement components (C3 and C5) measured by standard haemolytic assay. Serum levels of C3 and C5 were depressed in all strains of mice infected with P. berghei. There was no difference in the infectivity and course of P. berghei infection in the co-isogenic C5 deficient and non-deficient strains. The significance of these observations is discussed.", "contents": "Role of complement components in the susceptibility to Plasmodium berghei infection among inbred strains of mice. Inbred strains of mice, sensitive and resistant to Plasmodium berghei infections, including a complement deficient strain were infected with P. berghei. Daily levels of parasitaemia were determined and serum levels of complement components (C3 and C5) measured by standard haemolytic assay. Serum levels of C3 and C5 were depressed in all strains of mice infected with P. berghei. There was no difference in the infectivity and course of P. berghei infection in the co-isogenic C5 deficient and non-deficient strains. The significance of these observations is discussed."}
{"id": "PMID:1098587", "title": "Chloroquine resistance of Plasmodium falciparum in West Irian and East Kalimantan.", "content": "Evidence is presented of the chloroquine-resistance in Plasmodium falciparum in West Irian and East Kalimantan as shown by clinical observation and quantitative parasite counts in two patients hospitalized in Tokyo where no natural transmission of malaria occurs. The evidence is supported by the determination of plasma chloroquine levels, and in vitro sensitivity tests. The highest plasma level of chloroquine in the patient infected in West Irian was 197 ng/ml (fourth treatment) and 435 ng/ml (fifth treatment), and that in the patient infected in East Kalimantan was 312 ng/ml. Fifty percent inhibition of maturation was obtained in vitro at 0-6 and 0-62 nM/ml of chloroquine, respectively. Both patients were readically cured by 2 g of sulphadoxine and 100 mg of pyrimethamine given in a single dose.", "contents": "Chloroquine resistance of Plasmodium falciparum in West Irian and East Kalimantan. Evidence is presented of the chloroquine-resistance in Plasmodium falciparum in West Irian and East Kalimantan as shown by clinical observation and quantitative parasite counts in two patients hospitalized in Tokyo where no natural transmission of malaria occurs. The evidence is supported by the determination of plasma chloroquine levels, and in vitro sensitivity tests. The highest plasma level of chloroquine in the patient infected in West Irian was 197 ng/ml (fourth treatment) and 435 ng/ml (fifth treatment), and that in the patient infected in East Kalimantan was 312 ng/ml. Fifty percent inhibition of maturation was obtained in vitro at 0-6 and 0-62 nM/ml of chloroquine, respectively. Both patients were readically cured by 2 g of sulphadoxine and 100 mg of pyrimethamine given in a single dose."}
{"id": "PMID:1098588", "title": "Purification and some properties of malarial pigment.", "content": "Malarial pigment from erythrocytes infected with Plasmodium berghei was purified by treatment with sodium dodecyl sulphate solution, followed by incubation with Pancreatin. The purified pigment retained the apparently crystalline form of pigment within the parasite, rotated polarised light and had the same solubility characterisation as crude malarial pigment. It contained about 1% iron, all of which could be accounted for in terms of haemin. The iron of the pigment molecule is oxidised by the parasite to the ferric state.", "contents": "Purification and some properties of malarial pigment. Malarial pigment from erythrocytes infected with Plasmodium berghei was purified by treatment with sodium dodecyl sulphate solution, followed by incubation with Pancreatin. The purified pigment retained the apparently crystalline form of pigment within the parasite, rotated polarised light and had the same solubility characterisation as crude malarial pigment. It contained about 1% iron, all of which could be accounted for in terms of haemin. The iron of the pigment molecule is oxidised by the parasite to the ferric state."}
{"id": "PMID:1098589", "title": "Immunological studies in rodent malaria. I: Protective immunity induced in mice by mild strains of Plasmodium berghei yoelii against a virulent and fatal line of this plasmodium.", "content": "Mild and virulent lines of Plasmodium berghei yoelii are readily distinguished both by their course of infection and by the preference of the virulent line for mature red blood cells. Mice given either mild line were fully protected against the virulent P.b. yoelii one week after they had become negative. Mice given the mild line of P.b. yoelii 17X were fully protected against a challenge by the virulent line on the third day of infection (D+3). Mice given the mild and virulent lines of P.b. yoelii 17X concomitantly showed only slight protection, all eventually succumbing. Mice given the virulent P.b. yoelii 17X and challenged with P.b. yoelii 17X mild on D+1 showed 2/6 with virulent-like infections and 4/6 infections which resembled the mild controls. Five out of eleven animals challenged with P.b. berghei after immunization with mild P.b. yoelii had infections which resembled the P.b. berghei control group and were dead by D+23. The other six mice became negative, but all of them relapsed and three of them subsequently died. In mice challenged four weeks after their mild P.b. yoelii 17X infections had become negative, 5/6 succumbed to a P.b. berghei infection. Mice immunized with P.b. yoelii 17X mild and challenged with P.v. vinckei one week after becoming negative had a survival rate of 2/10.", "contents": "Immunological studies in rodent malaria. I: Protective immunity induced in mice by mild strains of Plasmodium berghei yoelii against a virulent and fatal line of this plasmodium. Mild and virulent lines of Plasmodium berghei yoelii are readily distinguished both by their course of infection and by the preference of the virulent line for mature red blood cells. Mice given either mild line were fully protected against the virulent P.b. yoelii one week after they had become negative. Mice given the mild line of P.b. yoelii 17X were fully protected against a challenge by the virulent line on the third day of infection (D+3). Mice given the mild and virulent lines of P.b. yoelii 17X concomitantly showed only slight protection, all eventually succumbing. Mice given the virulent P.b. yoelii 17X and challenged with P.b. yoelii 17X mild on D+1 showed 2/6 with virulent-like infections and 4/6 infections which resembled the mild controls. Five out of eleven animals challenged with P.b. berghei after immunization with mild P.b. yoelii had infections which resembled the P.b. berghei control group and were dead by D+23. The other six mice became negative, but all of them relapsed and three of them subsequently died. In mice challenged four weeks after their mild P.b. yoelii 17X infections had become negative, 5/6 succumbed to a P.b. berghei infection. Mice immunized with P.b. yoelii 17X mild and challenged with P.v. vinckei one week after becoming negative had a survival rate of 2/10."}
{"id": "PMID:1098590", "title": "The chemotherapy of rodent malaria, XXIII Causal prophylaxis, part II: Practical experience with Plasmodium yoelii nigeriensis in drug screening.", "content": "Data are presented on the causal prophylactic action of about 100 compounds of various types against Plasmodium yoelii nigeriensis N67 in mice. Examples are given to show how action against pre-erythrocytic schizonts may be differentiated from action on emerging erythrocytic stages. In a series of 35 8-aminoquinolines, all but 10 showed definite causal prophylactic activity at tolerated doses. The data permit the compounds to be ranked in order of activity, and many are shown to be more active in this test system than primaquine. Marked causal prophylactic activity is displayed by a variety of quinone structures, several of which show a significant residual action on blood stages. A high level of activity is found in dihydrofolate reductase inhibitors within several chemical classes. Rorguanil is more effective as a causal prophylactic than a blood schizontocide in the mouse as in man. Sulphonamides and sulphones are also effective in this system. The active levels are influenced by the content of PABA in the diet of the hosts. Causal prophylactic action has been detected in a number of experimental compounds including some antibiotics (such as tetracycline and clindamycin). The pyrocatechol RC 12 shows only slight activity at the maximum tolerated dose. Chloroquine, mepacrine, quinine, quinolinemethanols and phenanthrenemethanols are inactive as causal prophylactics. It is concluded that a rodent malaria-mouse model does provide a relatively simple model for the screening of drugs for causal prophylaxis, and the data so obtained are of relevance to the detection of causal prophylactics against human malaria.", "contents": "The chemotherapy of rodent malaria, XXIII Causal prophylaxis, part II: Practical experience with Plasmodium yoelii nigeriensis in drug screening. Data are presented on the causal prophylactic action of about 100 compounds of various types against Plasmodium yoelii nigeriensis N67 in mice. Examples are given to show how action against pre-erythrocytic schizonts may be differentiated from action on emerging erythrocytic stages. In a series of 35 8-aminoquinolines, all but 10 showed definite causal prophylactic activity at tolerated doses. The data permit the compounds to be ranked in order of activity, and many are shown to be more active in this test system than primaquine. Marked causal prophylactic activity is displayed by a variety of quinone structures, several of which show a significant residual action on blood stages. A high level of activity is found in dihydrofolate reductase inhibitors within several chemical classes. Rorguanil is more effective as a causal prophylactic than a blood schizontocide in the mouse as in man. Sulphonamides and sulphones are also effective in this system. The active levels are influenced by the content of PABA in the diet of the hosts. Causal prophylactic action has been detected in a number of experimental compounds including some antibiotics (such as tetracycline and clindamycin). The pyrocatechol RC 12 shows only slight activity at the maximum tolerated dose. Chloroquine, mepacrine, quinine, quinolinemethanols and phenanthrenemethanols are inactive as causal prophylactics. It is concluded that a rodent malaria-mouse model does provide a relatively simple model for the screening of drugs for causal prophylaxis, and the data so obtained are of relevance to the detection of causal prophylactics against human malaria."}
{"id": "PMID:1098591", "title": "A comparison of pigment from Schistosoma mansoni and Plasmodium berghei.", "content": "The structures of malarial and schistosomal pigment within the parasites are distinct. The two pigments are distinguishable both before and after extraction from host liver.", "contents": "A comparison of pigment from Schistosoma mansoni and Plasmodium berghei. The structures of malarial and schistosomal pigment within the parasites are distinct. The two pigments are distinguishable both before and after extraction from host liver."}
{"id": "PMID:1098592", "title": "Serodiagnosis by fluorescent antibody staining of an outbreak of trichinosis in Lebanon.", "content": "The opportunity offered by a recent outbreak of trichinosis in Lebannon was utilized to evaluate the applicability of an indirect fluorescent antibody (IFA) test for early diagnosis. Freeze-dried T. spiralis sonicated larval antigen fragments were used, together with human test serum and monospecific anti-human IgG, IgM and IgA fluorescein conjugates. Diagnostic IgG levels of 1/100 and above were observed in serum obtained some time during the acute phase of illness from nine out of ten patients. Diagnosis was made in seven cases within 30 days after the onset of symptoms. IgM antibodies were demonstrated in occasional samples. No IgA antibodies were detected. IgG levels of 1/100 were present in only four out of ten samples obtained 150 days after the clinical onset. These observations suggest that freeze-dried larval antigens make the IFA test an easy and useful procedure in the diagnosis of acute trichinosis. In chronic cases the efficacy of the method appears to be poor.", "contents": "Serodiagnosis by fluorescent antibody staining of an outbreak of trichinosis in Lebanon. The opportunity offered by a recent outbreak of trichinosis in Lebannon was utilized to evaluate the applicability of an indirect fluorescent antibody (IFA) test for early diagnosis. Freeze-dried T. spiralis sonicated larval antigen fragments were used, together with human test serum and monospecific anti-human IgG, IgM and IgA fluorescein conjugates. Diagnostic IgG levels of 1/100 and above were observed in serum obtained some time during the acute phase of illness from nine out of ten patients. Diagnosis was made in seven cases within 30 days after the onset of symptoms. IgM antibodies were demonstrated in occasional samples. No IgA antibodies were detected. IgG levels of 1/100 were present in only four out of ten samples obtained 150 days after the clinical onset. These observations suggest that freeze-dried larval antigens make the IFA test an easy and useful procedure in the diagnosis of acute trichinosis. In chronic cases the efficacy of the method appears to be poor."}
{"id": "PMID:1098593", "title": "Food and water intake in gold thioglucose-induced obese Charles River mice.", "content": "Food and water intake have been measured during the dynamic phase of gold thioglucose-induced obesity in Charles River mice. Regressions of gain in weight with food and water consumption were calculated in young growing animals and in adults fed ad libitum. The influence of fat content in the diet (2.5 and 8% fat) and environmental temperature (68degrees or 79degrees F) was estimated on the regressions. Excessive gain in weight without hyperphagia was observed in growing animals, in adults fed on a fat-enriched diet or maintained within a thermoneutral environment (79degrees F). But a significant hyperphagia was observed in adults fed with a conventional diet and maintained at 68degrees F or in growing animals as a sequela of food deprivation.", "contents": "Food and water intake in gold thioglucose-induced obese Charles River mice. Food and water intake have been measured during the dynamic phase of gold thioglucose-induced obesity in Charles River mice. Regressions of gain in weight with food and water consumption were calculated in young growing animals and in adults fed ad libitum. The influence of fat content in the diet (2.5 and 8% fat) and environmental temperature (68degrees or 79degrees F) was estimated on the regressions. Excessive gain in weight without hyperphagia was observed in growing animals, in adults fed on a fat-enriched diet or maintained within a thermoneutral environment (79degrees F). But a significant hyperphagia was observed in adults fed with a conventional diet and maintained at 68degrees F or in growing animals as a sequela of food deprivation."}
{"id": "PMID:1098594", "title": "Further evaluation of quinestrol in the inhibition of lactation: a double-blind comparison of two dose levels against placebo.", "content": "One hundred and ninetysix post-partum women, in whom lactation was to be prevented, were given under double-blind conditions either placebo or quinestrol 2 mg or 4 mg as a single oral dose within twentyfour hours of delivery. Early assessment of the results gave a failure rate of 58 per cent, 15 per cent and 5 per cent respectively, with statistically significant differences among the three groups of patients. At the follow-up evaluation, which could be made in only about one third of the women, breast troubles were recorded in 20 to 30 per cent, without significant differences among the groups. Post-partum amenorrhea showed a progressive prolongation from an average of 47.9 days in the controls to 64.6 and 72.6 days respectively in the 2 and 4 mg quinestrol groups. Adverse reactions, represented by delayed uterine involution during hospital stay and abnormal uterine bleeding in the late puerperium, were somewhat more frequent in the higher dose group than in the lower dose and control groups. On the basis of the prsent findings and of similar, though rare, experiences reported in the relevant literature, the question is therefore raised whether the 2 mg quinestrol dose would not be preferable to the 4 mg one for routine use in post-partum nonusing women.", "contents": "Further evaluation of quinestrol in the inhibition of lactation: a double-blind comparison of two dose levels against placebo. One hundred and ninetysix post-partum women, in whom lactation was to be prevented, were given under double-blind conditions either placebo or quinestrol 2 mg or 4 mg as a single oral dose within twentyfour hours of delivery. Early assessment of the results gave a failure rate of 58 per cent, 15 per cent and 5 per cent respectively, with statistically significant differences among the three groups of patients. At the follow-up evaluation, which could be made in only about one third of the women, breast troubles were recorded in 20 to 30 per cent, without significant differences among the groups. Post-partum amenorrhea showed a progressive prolongation from an average of 47.9 days in the controls to 64.6 and 72.6 days respectively in the 2 and 4 mg quinestrol groups. Adverse reactions, represented by delayed uterine involution during hospital stay and abnormal uterine bleeding in the late puerperium, were somewhat more frequent in the higher dose group than in the lower dose and control groups. On the basis of the prsent findings and of similar, though rare, experiences reported in the relevant literature, the question is therefore raised whether the 2 mg quinestrol dose would not be preferable to the 4 mg one for routine use in post-partum nonusing women."}
{"id": "PMID:1098595", "title": "Recurrence of idiopathic membranous nephropathy in a renal allograft.", "content": "A middle-aged man with idiopathic membranous nephropathy, who developed chronic renal failure, received a cadaver renal allograft. Two months later, massive, persistent proteinuria developed. A biopsy specimen of the allograft showed changes characteristic of membranous nephropathy and identical to those present in the patient's own kidney. The criteria for recurrence of glomerulonephritis in renal allografts are met in this case.", "contents": "Recurrence of idiopathic membranous nephropathy in a renal allograft. A middle-aged man with idiopathic membranous nephropathy, who developed chronic renal failure, received a cadaver renal allograft. Two months later, massive, persistent proteinuria developed. A biopsy specimen of the allograft showed changes characteristic of membranous nephropathy and identical to those present in the patient's own kidney. The criteria for recurrence of glomerulonephritis in renal allografts are met in this case."}
{"id": "PMID:1098596", "title": "Treatment of tachyarrhythmias by pacing.", "content": "The present status of the use of electrical pacing to treat tachyarrythmias is reviewed. The pathogenesis of arrythmia formation, methods of pacing, response of specific tachyarrhythmias to pacing and the clinical application of pacing to terminate and suppress tachyarrhythmias are delineated.", "contents": "Treatment of tachyarrhythmias by pacing. The present status of the use of electrical pacing to treat tachyarrythmias is reviewed. The pathogenesis of arrythmia formation, methods of pacing, response of specific tachyarrhythmias to pacing and the clinical application of pacing to terminate and suppress tachyarrhythmias are delineated."}
{"id": "PMID:1098598", "title": "Composition of cellulin, the unique chitin-glucan granules of the fungus, Apodachlya sp.", "content": "Cellulin granules, the polysaccharide inclusions found uniquely in oomycetous fungi of the order Leptomitales, were isolated from Apodachyla sp. The granules were prepared free of cell wall and cytoplasmic contaminants. Biochemical analyses and X-ray diffraction studies demonstrated that the granules were composed of 60% chitin and 39% glucan consisting of beta-1,3- and beta-1,6-linked glucose units. A protein content of only 0.1% was attributed to an insignificant amount of cytoplasmic contamination. Isolated granules and those in situ showed no apparent differences in their microscopic form.", "contents": "Composition of cellulin, the unique chitin-glucan granules of the fungus, Apodachlya sp. Cellulin granules, the polysaccharide inclusions found uniquely in oomycetous fungi of the order Leptomitales, were isolated from Apodachyla sp. The granules were prepared free of cell wall and cytoplasmic contaminants. Biochemical analyses and X-ray diffraction studies demonstrated that the granules were composed of 60% chitin and 39% glucan consisting of beta-1,3- and beta-1,6-linked glucose units. A protein content of only 0.1% was attributed to an insignificant amount of cytoplasmic contamination. Isolated granules and those in situ showed no apparent differences in their microscopic form."}
{"id": "PMID:1098599", "title": "Biosynthesis of sulphur amoni acids in Saccharomyces cerevisiae. I. Genetic analysis of leaky mutants of sulphite reductase.", "content": "The hypothesis of an alternative pathway of sulphur amino acid synthesis as the basis of the prototrophy of sulphite reductase negative (Sr--) strains of Saccharomyces cerevisiae has been rejected. Met- mutants obtained after phenylmercuric nitrate treatment of Sr- strains accumulate H2S as the consequence of a metabolic block which leads to methionine auxotrophy. This mutation has been shown to be independent of the Sr locus. We assume that the molecular basis of the prototrophy of Sr- mutant resides in a leaky missense induced in the Sr gene.", "contents": "Biosynthesis of sulphur amoni acids in Saccharomyces cerevisiae. I. Genetic analysis of leaky mutants of sulphite reductase. The hypothesis of an alternative pathway of sulphur amino acid synthesis as the basis of the prototrophy of sulphite reductase negative (Sr--) strains of Saccharomyces cerevisiae has been rejected. Met- mutants obtained after phenylmercuric nitrate treatment of Sr- strains accumulate H2S as the consequence of a metabolic block which leads to methionine auxotrophy. This mutation has been shown to be independent of the Sr locus. We assume that the molecular basis of the prototrophy of Sr- mutant resides in a leaky missense induced in the Sr gene."}
{"id": "PMID:1098600", "title": "The fine structure of Herpetosiphon, and a note on the taxonomy of the genus.", "content": "The fine structure of the Gram-negative filamentous gliding bacterium, Herpetosiphon is described. The outer membrane of the cell envelope could not be resolved as a separate structure, probably because it is fused with the underlying dense (peptidoglycan) layer. There was an additional wall layer outside this membrane-peptidoglycan complex, but a sheath in the classical sense, as postulated in the definition of the genus, was lacking. On the cell surface a loose network of fibrils could be seen. Inside the cells 3 types of intracytoplasmic membranes were discernible: a) true mesosomes near cross walls; b) a system of coarser membranes which was not connected with the septa and formed networks or tubular complexes; c) degenerated septa within bulbs. The bulbs are swollen sections of filaments, occurred mainly in ageing cultures, and are probably a degeneration phenomenon. The filaments contained necridia, i.e. dead and empty cells, across which breaks may occur so that empty cell wall cylinders remain attached to the ends of the daughter filaments, falsely suggesting the presence of a sheath. The taxonomy of Herpetosiphon is discussed in detail: The organism has been described before as Flexibacter giganteus. It is proposed to abandon the species H. aurantiacus in favor of H. giganteus, but to retain the genus Herpetosiphon. An improved definition of the genus is given.", "contents": "The fine structure of Herpetosiphon, and a note on the taxonomy of the genus. The fine structure of the Gram-negative filamentous gliding bacterium, Herpetosiphon is described. The outer membrane of the cell envelope could not be resolved as a separate structure, probably because it is fused with the underlying dense (peptidoglycan) layer. There was an additional wall layer outside this membrane-peptidoglycan complex, but a sheath in the classical sense, as postulated in the definition of the genus, was lacking. On the cell surface a loose network of fibrils could be seen. Inside the cells 3 types of intracytoplasmic membranes were discernible: a) true mesosomes near cross walls; b) a system of coarser membranes which was not connected with the septa and formed networks or tubular complexes; c) degenerated septa within bulbs. The bulbs are swollen sections of filaments, occurred mainly in ageing cultures, and are probably a degeneration phenomenon. The filaments contained necridia, i.e. dead and empty cells, across which breaks may occur so that empty cell wall cylinders remain attached to the ends of the daughter filaments, falsely suggesting the presence of a sheath. The taxonomy of Herpetosiphon is discussed in detail: The organism has been described before as Flexibacter giganteus. It is proposed to abandon the species H. aurantiacus in favor of H. giganteus, but to retain the genus Herpetosiphon. An improved definition of the genus is given."}
{"id": "PMID:1098601", "title": "Cytochemical detection of polysaccharides on the surface of the cell membrane complex in fungi.", "content": "Cytochemical staining in toto (periodic acid, thiosemicarbazide, OSO4) revealed the presence of polysaccharide lamellae on the surface of the cell membrane complex of fungi. The membraneous clusters in the vacuolar bodies of Claviceps purpurea were covered with these lamellae at both surfaces, as it was also the case with the endoplasmic reticulum membranes, the tonoplast and the cytoplasmic membrane. In Saccharomyces cerevisiae, the polysaccharide lamellae were visible on the surface of the endoplasmic reticulum membranes and the plasmalemma; the strain revealed polysaccharide deposits also on the tonoplasts of small vacuoles and in glucanase vesicles. We assume that these observations give precision to the localization of the enzymes synthetizing the glycoprotein components of the fungal cell wall.", "contents": "Cytochemical detection of polysaccharides on the surface of the cell membrane complex in fungi. Cytochemical staining in toto (periodic acid, thiosemicarbazide, OSO4) revealed the presence of polysaccharide lamellae on the surface of the cell membrane complex of fungi. The membraneous clusters in the vacuolar bodies of Claviceps purpurea were covered with these lamellae at both surfaces, as it was also the case with the endoplasmic reticulum membranes, the tonoplast and the cytoplasmic membrane. In Saccharomyces cerevisiae, the polysaccharide lamellae were visible on the surface of the endoplasmic reticulum membranes and the plasmalemma; the strain revealed polysaccharide deposits also on the tonoplasts of small vacuoles and in glucanase vesicles. We assume that these observations give precision to the localization of the enzymes synthetizing the glycoprotein components of the fungal cell wall."}
{"id": "PMID:1098602", "title": "The control by respiration of the uptake of alpha-methyl glucoside in Escherichia coli K12.", "content": "The uptake of methyl alpha-D-glucopyranoside (alpha-MG) by Escherichia coli K12 was decreased by the addition of substrates which stimulated the rate of oxygen consumption by the cells. The inhibition, which occurred only at non-saturating concentrations of alpha-MG, was not the result of a stimulation of the rate of exit of intracellular alpha-MG, and was abolished by the presence of carbonyl cyanide m-chlorophenylhydrazone or sodium azide. Since those drugs inhibit energy conservation at the respiratory chain and did not alter significantly the rate of oxygen consumption under the conditions for the assay of alpha-MG uptake, it appears that the inhibition of the transport system by respirable substrates is mediated by some form of energy derived from respiration.", "contents": "The control by respiration of the uptake of alpha-methyl glucoside in Escherichia coli K12. The uptake of methyl alpha-D-glucopyranoside (alpha-MG) by Escherichia coli K12 was decreased by the addition of substrates which stimulated the rate of oxygen consumption by the cells. The inhibition, which occurred only at non-saturating concentrations of alpha-MG, was not the result of a stimulation of the rate of exit of intracellular alpha-MG, and was abolished by the presence of carbonyl cyanide m-chlorophenylhydrazone or sodium azide. Since those drugs inhibit energy conservation at the respiratory chain and did not alter significantly the rate of oxygen consumption under the conditions for the assay of alpha-MG uptake, it appears that the inhibition of the transport system by respirable substrates is mediated by some form of energy derived from respiration."}
{"id": "PMID:1098603", "title": "Dependence of the maximum temperature for growth of Saccharomyces cerevisiae on nutrient concentration.", "content": "Saccharomyces cerevisae was grown in a chemostat under glucose limitation at three superoptimal temperatures.  In each steady state the specific growth rate was the sum of the dilution rate and the specific death rate, exponential death occurring with exponential growth.  The specific death rate was a function of both the temperature and the concentration of the limiting nutrient.  Each superoptimal temperature was characterized by a critical glucose concentration below which net growth was not possible.  The critical glucose concentration increased with the temperature.  Consequently the maximum temperature for growth was a function of the concentration of the limiting nutrient and approached the optimum temperature for growth with decreasing glucose concentrations.", "contents": "Dependence of the maximum temperature for growth of Saccharomyces cerevisiae on nutrient concentration. Saccharomyces cerevisae was grown in a chemostat under glucose limitation at three superoptimal temperatures.  In each steady state the specific growth rate was the sum of the dilution rate and the specific death rate, exponential death occurring with exponential growth.  The specific death rate was a function of both the temperature and the concentration of the limiting nutrient.  Each superoptimal temperature was characterized by a critical glucose concentration below which net growth was not possible.  The critical glucose concentration increased with the temperature.  Consequently the maximum temperature for growth was a function of the concentration of the limiting nutrient and approached the optimum temperature for growth with decreasing glucose concentrations."}
{"id": "PMID:1098604", "title": "[The development and the reversion of spheroplasts of a diaminopimelic acid-auxotrophic mutant of Escherichia coli].", "content": "1. The formation and reversion of spheroplasts of the diaminopimelic acid-auxotrophic mutant Escherichia coli K 12, 335, DAP-, R+TEM in a medium lacking diaminopimelic acid have been investigated by microphotography: During their development from rod form cells to spheroplasts cells on slide-surface-agar preparations underwent two successive cell divisions in the course of which the cells retained their rod form. The cells formed by these divisions partitioned into a varying number of spheroplasts of different size. The reversion of spheroplasts to rod form cells, started by the addition of diaminopimelic acid showed two characteristic steps: Each spheroplast partitioned again into several spheroplast-like cell bodies; most of them reverted directly to rod form cells. 2. The release of the R-factor mediated periplasmic TEM-beta-lactamase, E. C. 3.4.2.6., into the growth medium during the development of spheroplasts attained more than 50% of the entire TEM-beta=lactamase activity. The spheroplasts showed a multiple enhancement of TEM-beta-lactamase activity per mg cell protein compared with rod form cells.", "contents": "[The development and the reversion of spheroplasts of a diaminopimelic acid-auxotrophic mutant of Escherichia coli]. 1. The formation and reversion of spheroplasts of the diaminopimelic acid-auxotrophic mutant Escherichia coli K 12, 335, DAP-, R+TEM in a medium lacking diaminopimelic acid have been investigated by microphotography: During their development from rod form cells to spheroplasts cells on slide-surface-agar preparations underwent two successive cell divisions in the course of which the cells retained their rod form. The cells formed by these divisions partitioned into a varying number of spheroplasts of different size. The reversion of spheroplasts to rod form cells, started by the addition of diaminopimelic acid showed two characteristic steps: Each spheroplast partitioned again into several spheroplast-like cell bodies; most of them reverted directly to rod form cells. 2. The release of the R-factor mediated periplasmic TEM-beta-lactamase, E. C. 3.4.2.6., into the growth medium during the development of spheroplasts attained more than 50% of the entire TEM-beta=lactamase activity. The spheroplasts showed a multiple enhancement of TEM-beta-lactamase activity per mg cell protein compared with rod form cells."}
{"id": "PMID:1098605", "title": "Defining synchrony of cell cultures.", "content": "A method was developed for the statistical analysis of growth data from synchronized growth experiments. The analysis provided a firm basis for the recognition of synchrony and the objective graphical presentation of the growth pattern of a synchronized culture. The latter could then supply reliably the parameters required for the calculation of a synchronization index, i.e. for the synchrony evaluation.", "contents": "Defining synchrony of cell cultures. A method was developed for the statistical analysis of growth data from synchronized growth experiments. The analysis provided a firm basis for the recognition of synchrony and the objective graphical presentation of the growth pattern of a synchronized culture. The latter could then supply reliably the parameters required for the calculation of a synchronization index, i.e. for the synchrony evaluation."}
{"id": "PMID:1098606", "title": "Computers and psychiatry. Promises to keep.", "content": "The potential of computer-based psychiatric information systems is still largely undeveloped. Conceptual, technical, cost, and procedural problems have limited the impact of computers, but there are programs that have been successful or that show promise. While changes in the philosophy of psychiatric computer applications are on-going, a cost-effective, widely available clinical computer system has been developed.", "contents": "Computers and psychiatry. Promises to keep. The potential of computer-based psychiatric information systems is still largely undeveloped. Conceptual, technical, cost, and procedural problems have limited the impact of computers, but there are programs that have been successful or that show promise. While changes in the philosophy of psychiatric computer applications are on-going, a cost-effective, widely available clinical computer system has been developed."}
{"id": "PMID:1098607", "title": "Computer-assisted derivation of a screening interview for alcoholism.", "content": "A short screening interview for the diagnosis of alcoholism has been developed with the assistance of the automatic interaction detector (AID) program (a multivariate sequential analysis strategy) that is part of the OSIRIS statistical package. Retrospective and prospective tests of the effectiveness of the interview have shown that it permits a diagnosis of alcoholism with less effort than a larger, more comprehansive research interview. The performance of the screening and research interviews have been compared to assess the effects of severity of illness and reliability of individual replies on the diagnoses made by each interview.", "contents": "Computer-assisted derivation of a screening interview for alcoholism. A short screening interview for the diagnosis of alcoholism has been developed with the assistance of the automatic interaction detector (AID) program (a multivariate sequential analysis strategy) that is part of the OSIRIS statistical package. Retrospective and prospective tests of the effectiveness of the interview have shown that it permits a diagnosis of alcoholism with less effort than a larger, more comprehansive research interview. The performance of the screening and research interviews have been compared to assess the effects of severity of illness and reliability of individual replies on the diagnoses made by each interview."}
{"id": "PMID:1098608", "title": "Gonadotropin response to LH-RH in anorexia nervosa.", "content": "The gonadotropin-ovary axis of patients with anorexia nervosa was assessed with the aim of distinguishing between an intrinsically hypophysial deficiency and pituitary malfunction secondary to hypothalamic impairment. The most consistent endocrine abnormality was low serum luteinizing and follicle stimulating hormone (LH and FSH) levels associated with depressed serum estradiol levels. Gonadotropin levels responded to luteinizing hormone-releasing hormone (LH-RH), thus indicating a primarily hypothalamic dysfunction. The increase in serum FSH was at least as great as that of LH. The LH levels were additionally depressed and their increase after LH-RH injection somewhat blunted by three-day treatment with ethinyl estradiol.", "contents": "Gonadotropin response to LH-RH in anorexia nervosa. The gonadotropin-ovary axis of patients with anorexia nervosa was assessed with the aim of distinguishing between an intrinsically hypophysial deficiency and pituitary malfunction secondary to hypothalamic impairment. The most consistent endocrine abnormality was low serum luteinizing and follicle stimulating hormone (LH and FSH) levels associated with depressed serum estradiol levels. Gonadotropin levels responded to luteinizing hormone-releasing hormone (LH-RH), thus indicating a primarily hypothalamic dysfunction. The increase in serum FSH was at least as great as that of LH. The LH levels were additionally depressed and their increase after LH-RH injection somewhat blunted by three-day treatment with ethinyl estradiol."}
{"id": "PMID:1098610", "title": "[A simple method for breeding major spore quantities of Clostridium butyricum to be used in experiments for tumour diagnostics and therapy (author's transl)].", "content": "A simple method is described for breeding major spore quantities of Clostridium butyricum using a modification of Berger's iron bouillon. The method allows the breeding of cultures with comparatively high spore-forming rates which is essential for the use of such spores in experiments in the field of early tumour diagnostics and tumour therapy.", "contents": "[A simple method for breeding major spore quantities of Clostridium butyricum to be used in experiments for tumour diagnostics and therapy (author's transl)]. A simple method is described for breeding major spore quantities of Clostridium butyricum using a modification of Berger's iron bouillon. The method allows the breeding of cultures with comparatively high spore-forming rates which is essential for the use of such spores in experiments in the field of early tumour diagnostics and tumour therapy."}
{"id": "PMID:1098611", "title": "Organ preservation in success of cadaver transplants.", "content": "The long-term function of 110 cadaver kidney transplants preserved by hypothermic perfusion was compared with that of 79 fresh cadaver kidneys. The one-month failure rate was 4 percent in the perfused kidneys in contrast to a 32 percent one-month failure rate reported by one cooperative group. These data were again confirmed in a prospective, paired study comparing perfused and fresh cadaver kidneys from the same donor. Thus, perfusion of kidneys was not harmful for cadaver transplantation. In fact, of 83 first cadaver transplants, less than 30 percent of perfused kidneys failed in the first year. There appears to be no evidence supporting the idea that bloodless hypothermic perfusion permanently damages human kidneys or increases the chances of rejection.", "contents": "Organ preservation in success of cadaver transplants. The long-term function of 110 cadaver kidney transplants preserved by hypothermic perfusion was compared with that of 79 fresh cadaver kidneys. The one-month failure rate was 4 percent in the perfused kidneys in contrast to a 32 percent one-month failure rate reported by one cooperative group. These data were again confirmed in a prospective, paired study comparing perfused and fresh cadaver kidneys from the same donor. Thus, perfusion of kidneys was not harmful for cadaver transplantation. In fact, of 83 first cadaver transplants, less than 30 percent of perfused kidneys failed in the first year. There appears to be no evidence supporting the idea that bloodless hypothermic perfusion permanently damages human kidneys or increases the chances of rejection."}
{"id": "PMID:1098612", "title": "Renal trauma during laparotomy for intra-abdominal injury.", "content": "The advantages of nonsurgical or surgical management of renal injuries are important when the renal trauma is found during a laparotomy for intra-abdominal injury. Blunt external trauma caused 85.5 percent of the renal injuries found during laparotomy in 194 patients. A large dose or infusion intravenous pyelogram on a modified operating table has allowed immediate evaluation of the renal injury during a laparotomy. When the degree of renal injury was more severe, renal tissue and function were saved by early surgical management. In this group, lowered morbidity and a sharp reduction in delayed renal operations followed the introduction of immediate surgical management. The nephrectomy rate was 11 percent, which compares favorably with that of nonsurgical management. Clamping of the renal vessels prior to opening Gerota fascia prevented reactivation of hemorrhage and allowed for a deliberate operation with conservation of undamaged renal tissue.", "contents": "Renal trauma during laparotomy for intra-abdominal injury. The advantages of nonsurgical or surgical management of renal injuries are important when the renal trauma is found during a laparotomy for intra-abdominal injury. Blunt external trauma caused 85.5 percent of the renal injuries found during laparotomy in 194 patients. A large dose or infusion intravenous pyelogram on a modified operating table has allowed immediate evaluation of the renal injury during a laparotomy. When the degree of renal injury was more severe, renal tissue and function were saved by early surgical management. In this group, lowered morbidity and a sharp reduction in delayed renal operations followed the introduction of immediate surgical management. The nephrectomy rate was 11 percent, which compares favorably with that of nonsurgical management. Clamping of the renal vessels prior to opening Gerota fascia prevented reactivation of hemorrhage and allowed for a deliberate operation with conservation of undamaged renal tissue."}
{"id": "PMID:1098613", "title": "Clinical use of the membrane oxygenator.", "content": "Since April 1973 we have treated nine patients with extracorporeal membrane oxygenation (ECMO), utilizing the spiral coil membrane lung. One patient is a long-term survivor. All patients except one showed substantial improvement in peripheral arterial oxygen tension. Four adults and two neonates were treated for critical hypoxia. Two patients were treated for cardiac failure but failed to show improved myocardial function. Complications involving perfusion circuitry, cannulation, chronic systemic heparinization, thrombocytopenia, and renal failure have been managed with minimal difficulty. However, irreversible pulmonary, neurologic, hepatic, or gastrointestinal damage due to hypoxia present before the institution of ECMO was associated with lethal complications. The ECMO has supplied adequate oxygenation to this group of nine critically hypoxic patients. Institution of ECMO at an earlier date in patients with critical hypoxia would provide a higher likelihood of survival.", "contents": "Clinical use of the membrane oxygenator. Since April 1973 we have treated nine patients with extracorporeal membrane oxygenation (ECMO), utilizing the spiral coil membrane lung. One patient is a long-term survivor. All patients except one showed substantial improvement in peripheral arterial oxygen tension. Four adults and two neonates were treated for critical hypoxia. Two patients were treated for cardiac failure but failed to show improved myocardial function. Complications involving perfusion circuitry, cannulation, chronic systemic heparinization, thrombocytopenia, and renal failure have been managed with minimal difficulty. However, irreversible pulmonary, neurologic, hepatic, or gastrointestinal damage due to hypoxia present before the institution of ECMO was associated with lethal complications. The ECMO has supplied adequate oxygenation to this group of nine critically hypoxic patients. Institution of ECMO at an earlier date in patients with critical hypoxia would provide a higher likelihood of survival."}
{"id": "PMID:1098614", "title": "Alterations in kinins of coronary blood from rat heart homografts.", "content": "Forty-eight heterotopic heart transplants (33 homografts and 15 isografts) were performed in inbred rats. Levels of kallikrein, prekallikrein, and kallikrein inhibitor were determined in coronary sinus venous blood of grafted hearts at various intervals postgrafting. Homograft observations were assessed against those in nonrejecting isografts. Significant increases in homograft prekallikrein level on day 4 were followed by significant increases in homograft kallikrein activity on day 6. Kallikrein inhibitor levels in homografts were consistently increased in relation to isograft levels. Kallikrein and inhibitor activity showed an inverse relationship in their respective changes. High levels of kallikrein and prekallikrein occurred in homografts ten days posttransplantation. Immunological rejecting homografts showed meaningful changes in the activity of kinin-forming substances known to participate in inflammation.", "contents": "Alterations in kinins of coronary blood from rat heart homografts. Forty-eight heterotopic heart transplants (33 homografts and 15 isografts) were performed in inbred rats. Levels of kallikrein, prekallikrein, and kallikrein inhibitor were determined in coronary sinus venous blood of grafted hearts at various intervals postgrafting. Homograft observations were assessed against those in nonrejecting isografts. Significant increases in homograft prekallikrein level on day 4 were followed by significant increases in homograft kallikrein activity on day 6. Kallikrein inhibitor levels in homografts were consistently increased in relation to isograft levels. Kallikrein and inhibitor activity showed an inverse relationship in their respective changes. High levels of kallikrein and prekallikrein occurred in homografts ten days posttransplantation. Immunological rejecting homografts showed meaningful changes in the activity of kinin-forming substances known to participate in inflammation."}
{"id": "PMID:1098615", "title": "Use of a mechanical suturing apparatus in low colorectal anastomosis.", "content": "A circular stapling apparatus was especially designed for use in low colorectal anastomoses. In shape and size it resembles a slightly oversized proctosigmoidoscope. After the tumor-bearing segment is resected in the standard manner, the instrument is introduced via the anus through the distal segment into the abdomen. The proximal and distal segments are apposed and mechanically anastomosed. A secure anastomosis is thus performed more quickly and more reliably than by standard suture methods. The method has been used successfully in 20 dogs, without evidence of leakage or stenosis. In 165 human operations for low rectal carcinoma performed in Russia from 1967 to 1972, the mortality was 2.4% and the leakage rate was 3.6%. No stenosis or late stricture occurred.", "contents": "Use of a mechanical suturing apparatus in low colorectal anastomosis. A circular stapling apparatus was especially designed for use in low colorectal anastomoses. In shape and size it resembles a slightly oversized proctosigmoidoscope. After the tumor-bearing segment is resected in the standard manner, the instrument is introduced via the anus through the distal segment into the abdomen. The proximal and distal segments are apposed and mechanically anastomosed. A secure anastomosis is thus performed more quickly and more reliably than by standard suture methods. The method has been used successfully in 20 dogs, without evidence of leakage or stenosis. In 165 human operations for low rectal carcinoma performed in Russia from 1967 to 1972, the mortality was 2.4% and the leakage rate was 3.6%. No stenosis or late stricture occurred."}
{"id": "PMID:1098616", "title": "Treatment of flail chest. Use of intermittent mandatory ventilation and positive end-expiratory pressure.", "content": "For the past two years we have treated patients with flail chest injuries and concomitant respiratory failure with intermittent mandatory ventilation (IMV) and positive end-expiratory pressure (PEEP). Prior to 1972 these patients were treated with controlled mechanical ventilation (CMV) until gross flailing ceased and inspiratory force and vital capacity measurements were adequate. We retrospectively studied the charts of 37 consecutive patients to compare the length of mechanical ventilatory support of patients managed by conventional CMV with those ventilated with IMV and PEEP. The mean ventilation time of patients treated with IMV and PEEP (5.1 +/- 4.7 days) was significantly less than that of the patients treated with CMV (18.8 +/- 14.4 days) (P less than .001).", "contents": "Treatment of flail chest. Use of intermittent mandatory ventilation and positive end-expiratory pressure. For the past two years we have treated patients with flail chest injuries and concomitant respiratory failure with intermittent mandatory ventilation (IMV) and positive end-expiratory pressure (PEEP). Prior to 1972 these patients were treated with controlled mechanical ventilation (CMV) until gross flailing ceased and inspiratory force and vital capacity measurements were adequate. We retrospectively studied the charts of 37 consecutive patients to compare the length of mechanical ventilatory support of patients managed by conventional CMV with those ventilated with IMV and PEEP. The mean ventilation time of patients treated with IMV and PEEP (5.1 +/- 4.7 days) was significantly less than that of the patients treated with CMV (18.8 +/- 14.4 days) (P less than .001)."}
{"id": "PMID:1098617", "title": "Renal transplantation in the older age group.", "content": "Previously reported results of renal transplantation in the older age group have been discouraging; thus this form of therapy has generally been denied to patients over 50 years of age, unless a living related donor was available. A review of our transplant patients aged 51 or older who received cadaver donor kidneys was performed, and comparison was made to the survival being achieved for this group through hemodialysis or transplantation from living related donors. Functional survival of the homograft in the older age group in the Massachusetts General Hospital series compared favorably to that reported by the National Transplant Registry for recipients of all ages. We conclude that cadaver donor transplantation should be offered to increasing numbers of end-stage renal failure patients older than 50 years of age.", "contents": "Renal transplantation in the older age group. Previously reported results of renal transplantation in the older age group have been discouraging; thus this form of therapy has generally been denied to patients over 50 years of age, unless a living related donor was available. A review of our transplant patients aged 51 or older who received cadaver donor kidneys was performed, and comparison was made to the survival being achieved for this group through hemodialysis or transplantation from living related donors. Functional survival of the homograft in the older age group in the Massachusetts General Hospital series compared favorably to that reported by the National Transplant Registry for recipients of all ages. We conclude that cadaver donor transplantation should be offered to increasing numbers of end-stage renal failure patients older than 50 years of age."}
{"id": "PMID:1098618", "title": "Urologic problems in renal transplantation.", "content": "Of 206 patients who underwent 221 consecutive renal homografts, two patients had major urologic complications. One patient had a partial ureteric obstruction requiring surgical intervention and ureterostomy. Another patient had a fistula at the ureteropelvic junction secondary to vigorous diuresis in the presence of acute retention; eventually nephrectomy was required, but the patient survived. In one other instance, urinary extravasation was suspected without substantiation and with uneventful recovery of the patient. The total incidence of major urologic complications was less than 1%, with no related deaths.", "contents": "Urologic problems in renal transplantation. Of 206 patients who underwent 221 consecutive renal homografts, two patients had major urologic complications. One patient had a partial ureteric obstruction requiring surgical intervention and ureterostomy. Another patient had a fistula at the ureteropelvic junction secondary to vigorous diuresis in the presence of acute retention; eventually nephrectomy was required, but the patient survived. In one other instance, urinary extravasation was suspected without substantiation and with uneventful recovery of the patient. The total incidence of major urologic complications was less than 1%, with no related deaths."}
{"id": "PMID:1098619", "title": "Successful management of caliceal fistulas following renal transplantation.", "content": "Caliceal fistulas are unusual complications of renal transplantation. Segmental renal infarction and distal ureteral obstruction may be contributing factors in their formation. Two cases are reported, the first probably a result of segmental renal infarction from failure to recognize and anastomose an accessory renal artery, the second possibly related to blunt trauma to the area of the graft in an automobile accident and ureteral obstruction resulting from blood clots. Nephrostomy drainage through a healthy area of renal parenchyma was instrumental in the salvage of both kidneys.", "contents": "Successful management of caliceal fistulas following renal transplantation. Caliceal fistulas are unusual complications of renal transplantation. Segmental renal infarction and distal ureteral obstruction may be contributing factors in their formation. Two cases are reported, the first probably a result of segmental renal infarction from failure to recognize and anastomose an accessory renal artery, the second possibly related to blunt trauma to the area of the graft in an automobile accident and ureteral obstruction resulting from blood clots. Nephrostomy drainage through a healthy area of renal parenchyma was instrumental in the salvage of both kidneys."}
{"id": "PMID:1098620", "title": "Survival of cardiac xenografts. Effect of antithymocyte serum and enhancing heteroantiserum.", "content": "Rat hearts were transplanted to the abdomens of mice by primary vascular union, and sinus rhythm occurred a few minutes after perfusion of the rat coronary vessels with mouse arterial blood. Five groups of mice with rat heart transplants were studied to evaluate the effect of mouse antirat serum (MARS) and antithymocyte serum (ATS) on the survival of the xenografted hearts. When high-dose MARS was administered, hyperacute rejection occurred, while the administration of low-dose MARS doubled the survival time of the xenotransplant. Antithymocyte serum greatly prolonged the function and survival of the hearts, while the addition of \"enhancing serum\" to the ATS regimen did not procure longer survival over that achieved by ATS alone.", "contents": "Survival of cardiac xenografts. Effect of antithymocyte serum and enhancing heteroantiserum. Rat hearts were transplanted to the abdomens of mice by primary vascular union, and sinus rhythm occurred a few minutes after perfusion of the rat coronary vessels with mouse arterial blood. Five groups of mice with rat heart transplants were studied to evaluate the effect of mouse antirat serum (MARS) and antithymocyte serum (ATS) on the survival of the xenografted hearts. When high-dose MARS was administered, hyperacute rejection occurred, while the administration of low-dose MARS doubled the survival time of the xenotransplant. Antithymocyte serum greatly prolonged the function and survival of the hearts, while the addition of \"enhancing serum\" to the ATS regimen did not procure longer survival over that achieved by ATS alone."}
{"id": "PMID:1098621", "title": "[Age characteristics of the structure of the human retina].", "content": "Cytological, histochemical and neurohistological methods were used to study the human retina (27 corpses of people from 60 to 80 years of age). Two retinas from people of 32 and 27 years served as controls. The activity of alkaline phosphatase in neurons was established to increases with age. There were also enlargement of cysts and an increase of the square surface of their distribution in the retina, numerous aneurysmatic swelling along the course of the retinal capillaries. The nerve elements of the retina degenerated more often with age and partly died. Varicosities and excessively growing enlarged terminals were found along the course of dendritic and axonic processes. In the macular zone there were hypertrophicsynapses and circularly running sinuous fine fibres. Solitary ganglionic cells in this region had dendrites directed toward on side-they ere asymmetrical. The astrocytigglia was not changed. The fovea bottom contained may Muller's cells. Possible interpretation of the mechanism of appearance of vaicosities and excrescences in the nerve fibres with age age is presented.", "contents": "[Age characteristics of the structure of the human retina]. Cytological, histochemical and neurohistological methods were used to study the human retina (27 corpses of people from 60 to 80 years of age). Two retinas from people of 32 and 27 years served as controls. The activity of alkaline phosphatase in neurons was established to increases with age. There were also enlargement of cysts and an increase of the square surface of their distribution in the retina, numerous aneurysmatic swelling along the course of the retinal capillaries. The nerve elements of the retina degenerated more often with age and partly died. Varicosities and excessively growing enlarged terminals were found along the course of dendritic and axonic processes. In the macular zone there were hypertrophicsynapses and circularly running sinuous fine fibres. Solitary ganglionic cells in this region had dendrites directed toward on side-they ere asymmetrical. The astrocytigglia was not changed. The fovea bottom contained may Muller's cells. Possible interpretation of the mechanism of appearance of vaicosities and excrescences in the nerve fibres with age age is presented."}
{"id": "PMID:1098622", "title": "[Analysis of certain nodal features of regenerative reactions].", "content": "The increased degree of death of cells due to increased functional stresses may serve as a starting factor responsible for the compensatory growth. This fact allows considering reparative regeneration and compensatory growth as phenomena of the same class. Different forms of reparative growth (intracellular regeneration and proliferation of cells, endomorphosis and epimorphosis) serve to concrete tasks of reparation at different levels of organization of the morphological substrate. They are sustained by endogeneous morphogenetical mechanisms developing independently of other levels and of environment. In the subsystem of lover levels of organization the involved morphogenetical mechanisms may sustain not only compensation of the lost structures but also their overproduction. As a rule the program of reparative processes may be realized on higher (e. g. organic) level but partly. Among available criteria of regeneration at the present time the calculation of mitoses should be considered the most adequate one. The systemic approach may prove to be a perspective method both for estimation of regeneratory effect and for deeper analysis of reparative phenomena in general.", "contents": "[Analysis of certain nodal features of regenerative reactions]. The increased degree of death of cells due to increased functional stresses may serve as a starting factor responsible for the compensatory growth. This fact allows considering reparative regeneration and compensatory growth as phenomena of the same class. Different forms of reparative growth (intracellular regeneration and proliferation of cells, endomorphosis and epimorphosis) serve to concrete tasks of reparation at different levels of organization of the morphological substrate. They are sustained by endogeneous morphogenetical mechanisms developing independently of other levels and of environment. In the subsystem of lover levels of organization the involved morphogenetical mechanisms may sustain not only compensation of the lost structures but also their overproduction. As a rule the program of reparative processes may be realized on higher (e. g. organic) level but partly. Among available criteria of regeneration at the present time the calculation of mitoses should be considered the most adequate one. The systemic approach may prove to be a perspective method both for estimation of regeneratory effect and for deeper analysis of reparative phenomena in general."}
{"id": "PMID:1098623", "title": "[Simple method for the removal of the gelatin coating and the re-covering of histoautoradiographic preparations with an emulsion].", "content": "This simple method is used for removing emulsion from unsuccessful histoautoradiographic preparations for putting a new layer of emulsion. For this purpose the old emulsion is solved in warm water, the silver residue is removed from the preparation surface with a 10% solution of potassium ferricyanide.", "contents": "[Simple method for the removal of the gelatin coating and the re-covering of histoautoradiographic preparations with an emulsion]. This simple method is used for removing emulsion from unsuccessful histoautoradiographic preparations for putting a new layer of emulsion. For this purpose the old emulsion is solved in warm water, the silver residue is removed from the preparation surface with a 10% solution of potassium ferricyanide."}
{"id": "PMID:1098628", "title": "A suture-reinforced scleral sling. Technique for suspension of the ptotic upper lid.", "content": "A frontalis sling procedure for ptosis repair, employing preserved human bank sclera and polyfilament, cable-type, ophthalmic (Supramid Extra) suture in combination has been devised and tried. Results indicate that these two readily available materials that are now used separately may be combined for lid suspension. They are easy to work with and produce lasting results.", "contents": "A suture-reinforced scleral sling. Technique for suspension of the ptotic upper lid. A frontalis sling procedure for ptosis repair, employing preserved human bank sclera and polyfilament, cable-type, ophthalmic (Supramid Extra) suture in combination has been devised and tried. Results indicate that these two readily available materials that are now used separately may be combined for lid suspension. They are easy to work with and produce lasting results."}
{"id": "PMID:1098629", "title": "Bone formation in the normal human otic capsule.", "content": "Postmoretem examination of undecaicified temporal bones from five patients treated with tetracycline during adult life for unrelated disease showed the uptake of tetracycline label within the endochondral bone the otic capsule. Adult endochondral bone is histologically woven (nonlamellar) in character, and has been assumed to be metabolically inert in the healthy state. The strong perlvascular labelling seen suggests that new bone is normally deposited in the otic capsule and that this new bone is lamellar in character. Continuous new bone formation in the presence of a constant net volume of bone implies that  resorption must also occur normally within the otic capsule.", "contents": "Bone formation in the normal human otic capsule. Postmoretem examination of undecaicified temporal bones from five patients treated with tetracycline during adult life for unrelated disease showed the uptake of tetracycline label within the endochondral bone the otic capsule. Adult endochondral bone is histologically woven (nonlamellar) in character, and has been assumed to be metabolically inert in the healthy state. The strong perlvascular labelling seen suggests that new bone is normally deposited in the otic capsule and that this new bone is lamellar in character. Continuous new bone formation in the presence of a constant net volume of bone implies that  resorption must also occur normally within the otic capsule."}
{"id": "PMID:1098630", "title": "The brow-lift operation.", "content": "We describe a useful technique for local brow-lift operation. We discuss the results and outline procedural modifications that minimize postoperative scarring. The operation may be performed in conjunction with blepharoplasty or other cosmetic surgery.", "contents": "The brow-lift operation. We describe a useful technique for local brow-lift operation. We discuss the results and outline procedural modifications that minimize postoperative scarring. The operation may be performed in conjunction with blepharoplasty or other cosmetic surgery."}
{"id": "PMID:1098634", "title": "Evaluation of a single-layer intestinal anastomosis: an experimental study.", "content": "Fifteen single-layer and 15 double-layer inverting intestinal anastomoses were made in the small intestines of 15 healthy mongrel dogs and the results were compared. The single-layer anastomosis was found to be superior to the double-layer procedure in many respects.", "contents": "Evaluation of a single-layer intestinal anastomosis: an experimental study. Fifteen single-layer and 15 double-layer inverting intestinal anastomoses were made in the small intestines of 15 healthy mongrel dogs and the results were compared. The single-layer anastomosis was found to be superior to the double-layer procedure in many respects."}
{"id": "PMID:1098643", "title": "Algorithm for the multi-parameter analysis of nystagmus using a digital computer.", "content": "A computer program for analyzing nystagmus has been developed and can be used on a small laboratory digital computer. The algorithm accepts digitized data and looks for the minimum and maximum (minmax) points of the nystagmus waveform. These points in turn are used to define seven descriptive parameters of nystagmus, including the amplitude, duration, and velocity of the slow and fast phases, and the frequency. The algorithm uses three user-adjusted criteria for accepting or rejecting minmax points. The treatment of noisy or irregular data can be improved by adjusting the values of these criteria.", "contents": "Algorithm for the multi-parameter analysis of nystagmus using a digital computer. A computer program for analyzing nystagmus has been developed and can be used on a small laboratory digital computer. The algorithm accepts digitized data and looks for the minimum and maximum (minmax) points of the nystagmus waveform. These points in turn are used to define seven descriptive parameters of nystagmus, including the amplitude, duration, and velocity of the slow and fast phases, and the frequency. The algorithm uses three user-adjusted criteria for accepting or rejecting minmax points. The treatment of noisy or irregular data can be improved by adjusting the values of these criteria."}
{"id": "PMID:1098637", "title": "Causes of enlarged sternal bursas (breast blisters).", "content": "Bursas were taken weekly as normal turkeys developed from 3 to 22 weeks of age, and no specific bacterial or viral infection was found. Bacterial cultures were also made of condemned bursas taken at processing. Of 144 collected, 91% were sterile; the others had staphylococci, streptococci, or coliform organisms. Experiments with turkeys measured the effect of strain, body conformation, completeness of breast-feather cover, protection of the keel with sheepskins, age when put on range, and type of range on the incidence of enlarged sternal bursas. The strain difference of 25.4% breast blisters against 11.5% was highly significant (99% confidence). In one experiment the effect of feather cover was significant at the 95% level of confidence (61.7% breast blisters with no feather cover, versus 40% with complete feather cover). The effect of protecting the breast with sheepskin was significant at the 95% level of confidence in reducing incidence of breast blisters. There was no significant difference in the incidence of breast blisters in relation to body conformation, age, or type of range.", "contents": "Causes of enlarged sternal bursas (breast blisters). Bursas were taken weekly as normal turkeys developed from 3 to 22 weeks of age, and no specific bacterial or viral infection was found. Bacterial cultures were also made of condemned bursas taken at processing. Of 144 collected, 91% were sterile; the others had staphylococci, streptococci, or coliform organisms. Experiments with turkeys measured the effect of strain, body conformation, completeness of breast-feather cover, protection of the keel with sheepskins, age when put on range, and type of range on the incidence of enlarged sternal bursas. The strain difference of 25.4% breast blisters against 11.5% was highly significant (99% confidence). In one experiment the effect of feather cover was significant at the 95% level of confidence (61.7% breast blisters with no feather cover, versus 40% with complete feather cover). The effect of protecting the breast with sheepskin was significant at the 95% level of confidence in reducing incidence of breast blisters. There was no significant difference in the incidence of breast blisters in relation to body conformation, age, or type of range."}
{"id": "PMID:1098638", "title": "Influence of rofenaid-40 feed medication on an experimental Salmonella infection in chickens.", "content": "Rofenaid-40 administered at 0.02% in feed to chickens experimentally infected with Salmonella typhimurium resulted in less colonization and shedding of S. typhimurium than in unmedicated chickens. The antibacterial sensitivity pattern of S. typhimurium isolated from infected birds had not changed after exposure to Rofenaid for 56 days.", "contents": "Influence of rofenaid-40 feed medication on an experimental Salmonella infection in chickens. Rofenaid-40 administered at 0.02% in feed to chickens experimentally infected with Salmonella typhimurium resulted in less colonization and shedding of S. typhimurium than in unmedicated chickens. The antibacterial sensitivity pattern of S. typhimurium isolated from infected birds had not changed after exposure to Rofenaid for 56 days."}
{"id": "PMID:1098647", "title": "Quantitation in morphology: possibilities and limits.", "content": "Methods of structural measurement (morphometry) play an increasing role in investigative pathology. Basic methods of surface and volume measurement and their use in pathology are discussed. This paper demonstrates on the example of the pulmonary gas exchange apparatus that a morphometric approach, using methods of stereology, can yield results of high significance in assessing the structural alterations underlying a gas exchange deficiency.", "contents": "Quantitation in morphology: possibilities and limits. Methods of structural measurement (morphometry) play an increasing role in investigative pathology. Basic methods of surface and volume measurement and their use in pathology are discussed. This paper demonstrates on the example of the pulmonary gas exchange apparatus that a morphometric approach, using methods of stereology, can yield results of high significance in assessing the structural alterations underlying a gas exchange deficiency."}
{"id": "PMID:1098648", "title": "Glomerulopathies in human renal allografts.", "content": "The present study discusses the light, electron and immunofluorescence microscopy as well as some clinicopathologic correlations of rejection change in human renal allograft glomeruli. It is based on examination of 126 tissue specimens from 54 grafts obtained from 50 patients (1966-1973). The most frequent and characteristic lesion was membranous transplant glomerulopathy (MG) with irregular fibrillar thickening of capillary walls but without conspicuous hypercellularity. This thickening was caused by subendothelial depositsdifferent from classical fibrinoid lesions. During further progression, widening and peripheral extension of mesangium with degenerative changes became apparent. Advanced MG was encountered most frequently in the 2nd year after transplantation (TPL) at moderate to medium proteinuria and hypertension. It was accompanied by endarteristic rejection changes, and renal insufficiency set on usually in the course of the 3rd year. Nevertheless, the course, symptoms, and graft survival exhibited considerable variations. - The morphology and manifestations of destructive segmental transplant glomerulopathy  (SG) depended on the time of its development. In the early stage (within about 3 months after TPL), the lesion was characterized by areas of fibrinoid insudation and necro(bio)sis associated with severe vascular changes, most frequently obliterative arterio(lo)pathy (OA). The ultrastructure was characterized by endothelial defects with host's polynuclear reaction and focal intravascular coagulation. The grafts thus affected failed soon, their function usually subsiding within the first trimester  at a moderate, but gradually increasing proteinuria and severe persistent hypertension. The late from of destructive SG presenting as fibrohyaline obliteration of the loops with foam cells always accompanied advanced MG with severe arterial lesions. - Fluorescence microscopy revealed both linear and focal fixation of antisera, which, however had no apparent correlation with the microscopical and clinical presentations.", "contents": "Glomerulopathies in human renal allografts. The present study discusses the light, electron and immunofluorescence microscopy as well as some clinicopathologic correlations of rejection change in human renal allograft glomeruli. It is based on examination of 126 tissue specimens from 54 grafts obtained from 50 patients (1966-1973). The most frequent and characteristic lesion was membranous transplant glomerulopathy (MG) with irregular fibrillar thickening of capillary walls but without conspicuous hypercellularity. This thickening was caused by subendothelial depositsdifferent from classical fibrinoid lesions. During further progression, widening and peripheral extension of mesangium with degenerative changes became apparent. Advanced MG was encountered most frequently in the 2nd year after transplantation (TPL) at moderate to medium proteinuria and hypertension. It was accompanied by endarteristic rejection changes, and renal insufficiency set on usually in the course of the 3rd year. Nevertheless, the course, symptoms, and graft survival exhibited considerable variations. - The morphology and manifestations of destructive segmental transplant glomerulopathy  (SG) depended on the time of its development. In the early stage (within about 3 months after TPL), the lesion was characterized by areas of fibrinoid insudation and necro(bio)sis associated with severe vascular changes, most frequently obliterative arterio(lo)pathy (OA). The ultrastructure was characterized by endothelial defects with host's polynuclear reaction and focal intravascular coagulation. The grafts thus affected failed soon, their function usually subsiding within the first trimester  at a moderate, but gradually increasing proteinuria and severe persistent hypertension. The late from of destructive SG presenting as fibrohyaline obliteration of the loops with foam cells always accompanied advanced MG with severe arterial lesions. - Fluorescence microscopy revealed both linear and focal fixation of antisera, which, however had no apparent correlation with the microscopical and clinical presentations."}
{"id": "PMID:1098649", "title": "[Studies on the number of fat cells and connective tissue cells in white adipose tissue in chronic starvation and after refeeding. I. Investigations on aurothioglucose-obese NMRI-albino-mice(author's transl)].", "content": "Former investigations (Rakow et al., 1970, 1971 b) on epididymal fat pads of lean NMRI-Albino-mice during starvation and refeeding have shown that the number of fat cells (adipocytes) remained unchanged while the number of cells o connective tissue decreased during starvation and increased during the refeeding period. In the present paper the problem has been investigated whether these variations could be demonstrated also in NMRI-albino-mice obesified by administration of aurothioglucose. The investigations were performed with male NMRI-Albino-mice rendered obese by administration of aurothioglucose (800 mg per kg body weight). These animals were fed 2.5 g Altromin 1115 R daily for six weeks. After this they were fed Altromin 1115 R and additional oat flakes ad libitum for three (exp. groups HW3) and seven (exp. groups HW 7) days respectively. After this time the animals were sacrificed. The epididymal fat pads were removed and weighed. One of both pads of each animal was used for chemical investigations. After PCA-treatment (0.5 n, 90 degrees C. 15 min.) the DNA (Burton, 1956) and RNA (Ceriotti, 1955) was determined in the supernatant. After NaOH-treatment (0.5 n, 37 degrees C, 24 Hrs) the noncollagen protein content (Lowry et al., 1951) of the sediment was estimated. After HCl-treatment (25%, 110 degrees C, 20 hrs.) the collagen content (Stegemenn, 1958; modified by Rauskolb, 1967) was determined. The remaining fat pad was used for calculations of cell numbers in the fat cell and connective tissue cell compartment. For this reason fat cells were isolated according to Rodbell (1964). The fat cell diameters were determined microscopically and the average masses of the fat cells were estimated. From the wet weight of the fat pads and the average fat cell mass the number of fat cells was calculated. The remaining suspension of fat cells and cells of connective tissue was utilized for cell smears. These smears were stained with Schiff's reagent (Feulgen et al., 1924; Graumann, 1952). With an integrating microdensitometer (Deeley, 1955) the average relative DNA-content of single cell nuclei were measured and the ploidy patterns were estimated. From the whole DNA-content of the fat pads and the DNA-content of the fat cell population the number of cells of the connective tissue was calculated...", "contents": "[Studies on the number of fat cells and connective tissue cells in white adipose tissue in chronic starvation and after refeeding. I. Investigations on aurothioglucose-obese NMRI-albino-mice(author's transl)]. Former investigations (Rakow et al., 1970, 1971 b) on epididymal fat pads of lean NMRI-Albino-mice during starvation and refeeding have shown that the number of fat cells (adipocytes) remained unchanged while the number of cells o connective tissue decreased during starvation and increased during the refeeding period. In the present paper the problem has been investigated whether these variations could be demonstrated also in NMRI-albino-mice obesified by administration of aurothioglucose. The investigations were performed with male NMRI-Albino-mice rendered obese by administration of aurothioglucose (800 mg per kg body weight). These animals were fed 2.5 g Altromin 1115 R daily for six weeks. After this they were fed Altromin 1115 R and additional oat flakes ad libitum for three (exp. groups HW3) and seven (exp. groups HW 7) days respectively. After this time the animals were sacrificed. The epididymal fat pads were removed and weighed. One of both pads of each animal was used for chemical investigations. After PCA-treatment (0.5 n, 90 degrees C. 15 min.) the DNA (Burton, 1956) and RNA (Ceriotti, 1955) was determined in the supernatant. After NaOH-treatment (0.5 n, 37 degrees C, 24 Hrs) the noncollagen protein content (Lowry et al., 1951) of the sediment was estimated. After HCl-treatment (25%, 110 degrees C, 20 hrs.) the collagen content (Stegemenn, 1958; modified by Rauskolb, 1967) was determined. The remaining fat pad was used for calculations of cell numbers in the fat cell and connective tissue cell compartment. For this reason fat cells were isolated according to Rodbell (1964). The fat cell diameters were determined microscopically and the average masses of the fat cells were estimated. From the wet weight of the fat pads and the average fat cell mass the number of fat cells was calculated. The remaining suspension of fat cells and cells of connective tissue was utilized for cell smears. These smears were stained with Schiff's reagent (Feulgen et al., 1924; Graumann, 1952). With an integrating microdensitometer (Deeley, 1955) the average relative DNA-content of single cell nuclei were measured and the ploidy patterns were estimated. From the whole DNA-content of the fat pads and the DNA-content of the fat cell population the number of cells of the connective tissue was calculated..."}
{"id": "PMID:1098650", "title": "The role of bone marrow in different phases of the cellular repopulation of irradiated mouse thymus.", "content": "In one series of experiments, the cellular repopulation of the thymus was investigated in mice exposed first to 200 R on the whole body and, after various intervals, to 700 R with one leg protected or unprotected during the exposure. When no protection was made, the mice were transplanted with syngeneic bone marrow cells in a defined number immediately after irradiation. Repopulation was fastest when the interval between exposures was 5 days, and most delayed when it was 14 days; with a 30 day interval the speed of repopulation was intermediate, and resembled that of a control group exposed to only the second dose. In another experimental series, thymus repopulation was studied after exposure of mice first to 200 R with one leg protected or unprotected and, after an interval of 5, 14 or 30 days, to a second dose of 700 R on the whole body. In all cases, syngeneic bone marrow cells were grafted intravenously after the second irradiation. The thymus repopulation was enhanced by protection of the leg when 14 day interval separated the exposures. In the other cases, no enhancement was noted. The findings were interpreted to indicate that the cellular composition of the thymus and, in particular, the frequency of the proliferating stem cells at the time of the exposure determines thymic repopulation for about two weeks after irradiation. After this period, repopulation is due to new precursors from the bone marrow which seeded the thymus.", "contents": "The role of bone marrow in different phases of the cellular repopulation of irradiated mouse thymus. In one series of experiments, the cellular repopulation of the thymus was investigated in mice exposed first to 200 R on the whole body and, after various intervals, to 700 R with one leg protected or unprotected during the exposure. When no protection was made, the mice were transplanted with syngeneic bone marrow cells in a defined number immediately after irradiation. Repopulation was fastest when the interval between exposures was 5 days, and most delayed when it was 14 days; with a 30 day interval the speed of repopulation was intermediate, and resembled that of a control group exposed to only the second dose. In another experimental series, thymus repopulation was studied after exposure of mice first to 200 R with one leg protected or unprotected and, after an interval of 5, 14 or 30 days, to a second dose of 700 R on the whole body. In all cases, syngeneic bone marrow cells were grafted intravenously after the second irradiation. The thymus repopulation was enhanced by protection of the leg when 14 day interval separated the exposures. In the other cases, no enhancement was noted. The findings were interpreted to indicate that the cellular composition of the thymus and, in particular, the frequency of the proliferating stem cells at the time of the exposure determines thymic repopulation for about two weeks after irradiation. After this period, repopulation is due to new precursors from the bone marrow which seeded the thymus."}
{"id": "PMID:1098653", "title": "Specific cyanylation and cleavage at cysteine-104 human hemoglobin alpha-chain. A novel approach to the problem of the alpha-chain tryptic core in the study of haemoglobin variants by \"fingerprinting\" methods.", "content": "1. A new approach to the analysis, by \"fingerprinting\", of the tryptic core region of human haemoglobin alpha-chain is described. 2. The alpha-chain is cyanylated at its single cysteine residue (alpha104) and then split, by exposure to mild alkali, at the N-peptide bond of the resulting beta-thiocyanoalanine residue. 3. The two cleavage fragments, alpha1-103 and alpha104-141, are separated by gel filtration, and the fragment alpha104-141, which contains all the residues of the alpha-chain tryptic core, is digested with pepsin. 4. Preparative \"fingerprints\" of these peptic peptides yield eight major peptides, which provide complete sequence information for the whole region alpha104-141. 5. The utility of the method is demonstrated by repeating the determination of the substitution in haemoglobin Hopkins-2, a known alpha-chain core variant in which histidine-alpha112 (G19) is replaced by an aspartic acid residue.", "contents": "Specific cyanylation and cleavage at cysteine-104 human hemoglobin alpha-chain. A novel approach to the problem of the alpha-chain tryptic core in the study of haemoglobin variants by \"fingerprinting\" methods. 1. A new approach to the analysis, by \"fingerprinting\", of the tryptic core region of human haemoglobin alpha-chain is described. 2. The alpha-chain is cyanylated at its single cysteine residue (alpha104) and then split, by exposure to mild alkali, at the N-peptide bond of the resulting beta-thiocyanoalanine residue. 3. The two cleavage fragments, alpha1-103 and alpha104-141, are separated by gel filtration, and the fragment alpha104-141, which contains all the residues of the alpha-chain tryptic core, is digested with pepsin. 4. Preparative \"fingerprints\" of these peptic peptides yield eight major peptides, which provide complete sequence information for the whole region alpha104-141. 5. The utility of the method is demonstrated by repeating the determination of the substitution in haemoglobin Hopkins-2, a known alpha-chain core variant in which histidine-alpha112 (G19) is replaced by an aspartic acid residue."}
{"id": "PMID:1098654", "title": "Nitrogenase of Klebsiella pneumoniae. A stopped-flow study of magnesium-adenosine triphosphate-induce electron transfer between the compeonent proteins.", "content": "Stopped-flow kinetic data have been obtained for a rapid electron-transfer reaction between the component proteins of nitrogenase from Klebsiella pneumoniae, which was induced by MgATP. Up to three equivalents of the Fe-containing protein were rapidly oxidized by one equivalent of the Fe-Mo-containing protein in a unimolecular reaction, k2 = 2 x 10(2)S-1. Evidence for a tight complex between the component proteins, KD(complex) less than 0.5 muM, which was formed with a rate k1 greater than 1 x 10(7)M-1-S-1, has been obtained. MgATP bound to either the Fe-containing protein or to the two-protein complex with a rate k3 greater than 2.5 x 10(6)M-1-S-1 and with KD(MgATP) = 0.4mM, before the electron-transfer reaction.", "contents": "Nitrogenase of Klebsiella pneumoniae. A stopped-flow study of magnesium-adenosine triphosphate-induce electron transfer between the compeonent proteins. Stopped-flow kinetic data have been obtained for a rapid electron-transfer reaction between the component proteins of nitrogenase from Klebsiella pneumoniae, which was induced by MgATP. Up to three equivalents of the Fe-containing protein were rapidly oxidized by one equivalent of the Fe-Mo-containing protein in a unimolecular reaction, k2 = 2 x 10(2)S-1. Evidence for a tight complex between the component proteins, KD(complex) less than 0.5 muM, which was formed with a rate k1 greater than 1 x 10(7)M-1-S-1, has been obtained. MgATP bound to either the Fe-containing protein or to the two-protein complex with a rate k3 greater than 2.5 x 10(6)M-1-S-1 and with KD(MgATP) = 0.4mM, before the electron-transfer reaction."}
{"id": "PMID:1098655", "title": "The kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form Proteus vulgaris.", "content": "Km and Vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. Degradation of chondroitin 4-sulphate was inhibited by hyaluronic acid but not by keratan sulphate. The results are discussed with regard to the use to the use of chondroitinase as a sleective reagent for the degradation of tissue glycosaminoglycans.", "contents": "The kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form Proteus vulgaris. Km and Vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. Degradation of chondroitin 4-sulphate was inhibited by hyaluronic acid but not by keratan sulphate. The results are discussed with regard to the use to the use of chondroitinase as a sleective reagent for the degradation of tissue glycosaminoglycans."}
{"id": "PMID:1098656", "title": "Studies on the high-sulphur proteins of reduced mohair. The isolation and amino acid sequence of protein scmkb-m1.2.", "content": "The complete amino acid sequence of mohair protein, SCMKB-M1.2 (97 residues), was determined. The protein was isolated from reduced and carboxymethylated mohair by chromatography on DEAE-cellulose phosphate. Peptides for sequence determination were obtained by digestion with trypsin, pepsin, chymotrypsin, thermolysin and papain, and were fractionated by DEAE-cellulose chromatography, paper chromatography and electrophoresis. The sequence of the peptides were determined by the Edman degradation method (by use of both the Beckman Sequence and a non-automatic procedure), and by partial acid hydrolysis. The protein is closely homologous to wool protein SCMKB-IIIB2, and also contains acetylated alanine as N-terminal amino acid.", "contents": "Studies on the high-sulphur proteins of reduced mohair. The isolation and amino acid sequence of protein scmkb-m1.2. The complete amino acid sequence of mohair protein, SCMKB-M1.2 (97 residues), was determined. The protein was isolated from reduced and carboxymethylated mohair by chromatography on DEAE-cellulose phosphate. Peptides for sequence determination were obtained by digestion with trypsin, pepsin, chymotrypsin, thermolysin and papain, and were fractionated by DEAE-cellulose chromatography, paper chromatography and electrophoresis. The sequence of the peptides were determined by the Edman degradation method (by use of both the Beckman Sequence and a non-automatic procedure), and by partial acid hydrolysis. The protein is closely homologous to wool protein SCMKB-IIIB2, and also contains acetylated alanine as N-terminal amino acid."}
{"id": "PMID:1098657", "title": "Purification of D-alanine carboxypeptidase from Escherichia coli B on a penicillin-Sepharose column.", "content": "1. A soluble D-alanine carboxypeptidase from Escherichia coli strain B was purified on a p-aminobenzylpenicillin-Sepharose column. This one-step chromatography followed by an (NH4)2SO4 precipitation yielded an enzyme purified 1200-fold and some of its properties are reported. 2. The pure D-alanine carboxypeptidase was devoid of D-alanine carboxypeptidase II activity and migrated as a single protein band on analytical disc gel electrophoresis. 3. Triton X-100 in the purification procedure is an absolute requirement for obtaining a stable enzyme. 4. The enzymic activity of D-alanine carboxypeptidase was greatly affected in solution of high salt concentrations and varied somewhat with the nature of the cation tested.", "contents": "Purification of D-alanine carboxypeptidase from Escherichia coli B on a penicillin-Sepharose column. 1. A soluble D-alanine carboxypeptidase from Escherichia coli strain B was purified on a p-aminobenzylpenicillin-Sepharose column. This one-step chromatography followed by an (NH4)2SO4 precipitation yielded an enzyme purified 1200-fold and some of its properties are reported. 2. The pure D-alanine carboxypeptidase was devoid of D-alanine carboxypeptidase II activity and migrated as a single protein band on analytical disc gel electrophoresis. 3. Triton X-100 in the purification procedure is an absolute requirement for obtaining a stable enzyme. 4. The enzymic activity of D-alanine carboxypeptidase was greatly affected in solution of high salt concentrations and varied somewhat with the nature of the cation tested."}
{"id": "PMID:1098658", "title": "Insulin release and the microtubular system of the islets of Langerhans. Identification and characterization of tubulin-like protein.", "content": "1. Incubation of islets of Langerhans in vitro in the presence of colchicine produced a progressive inhibition of the insulin-secretory response to glucose, which was dependent on the time of incubation. 2. The uptake of [3-H]colchicine by islet cells was a rapid process, equilibrium being reached in less than 30 min. Part of the colchicine taken up was bound to protein material, which was recovered largely in a post-microsomal supernatant fraction prepared from the islets. In contrast with this rapid uptake, the binding of colchicine by islet-cell proteins in intact islets or in islet homogenates was a slow process, and equilibrium was not reached for 60-90 min. After an initial 30 min delay, the time-course of the binding of [3-H]colchicine to islet-cell proteins paralleled that for the inhibitory effect of colchicine on insulin release. 3. Some purification of the colchicine-binding material present in islet homogenates could be achieved by precipitation of the protein with 2mM-CaCl2 (2.8-fold). However, ion-exchange chromatography on DEAE-Sephadex produced a further 27-fold purification on elution with 0.6M-NaCl. 4. Colchicine-binding protein prepared from islets by ion-exchange chromatography showed an intrinsic association constant for colchicine of 1.4muM and an apparent molecular weight on gel filtration of 110000. 5. These results suggest that colchicine-binding protein in islet cells closely resembles tubulin extracted from the other tissues. The delayed effectiveness of colchicine in inhibiting insulin secretion is not due to poor penetration of colchicine into the cells but rather to slow binding of the alkaloid to islet-cell tubulin. It seems likely that, as in other tissues, this binding prevents polymerization of the tubulin into microtubules, and thus interferes with the release process.", "contents": "Insulin release and the microtubular system of the islets of Langerhans. Identification and characterization of tubulin-like protein. 1. Incubation of islets of Langerhans in vitro in the presence of colchicine produced a progressive inhibition of the insulin-secretory response to glucose, which was dependent on the time of incubation. 2. The uptake of [3-H]colchicine by islet cells was a rapid process, equilibrium being reached in less than 30 min. Part of the colchicine taken up was bound to protein material, which was recovered largely in a post-microsomal supernatant fraction prepared from the islets. In contrast with this rapid uptake, the binding of colchicine by islet-cell proteins in intact islets or in islet homogenates was a slow process, and equilibrium was not reached for 60-90 min. After an initial 30 min delay, the time-course of the binding of [3-H]colchicine to islet-cell proteins paralleled that for the inhibitory effect of colchicine on insulin release. 3. Some purification of the colchicine-binding material present in islet homogenates could be achieved by precipitation of the protein with 2mM-CaCl2 (2.8-fold). However, ion-exchange chromatography on DEAE-Sephadex produced a further 27-fold purification on elution with 0.6M-NaCl. 4. Colchicine-binding protein prepared from islets by ion-exchange chromatography showed an intrinsic association constant for colchicine of 1.4muM and an apparent molecular weight on gel filtration of 110000. 5. These results suggest that colchicine-binding protein in islet cells closely resembles tubulin extracted from the other tissues. The delayed effectiveness of colchicine in inhibiting insulin secretion is not due to poor penetration of colchicine into the cells but rather to slow binding of the alkaloid to islet-cell tubulin. It seems likely that, as in other tissues, this binding prevents polymerization of the tubulin into microtubules, and thus interferes with the release process."}
{"id": "PMID:1098659", "title": "Synthesis of alternative membrane-bound redox carriers during aerobic growth of Escherichia coli in the presence of potassium cyanide.", "content": "Aerobic growth of Escherichia coli with an oxidizable substrate as carbon source in the presence of low concentrations of KCN leads to the synthesis and integration into the membrane of menaquinone and cytochromes b558, a1 and d in addition to the redox carriers normally present under aerobic growth conditions, namely ubiquinone and cytochromes b562, b556 and o. The results are discussed with reference to other phenotypic and genotypic modifications to the electron-transport chains of E. coli.", "contents": "Synthesis of alternative membrane-bound redox carriers during aerobic growth of Escherichia coli in the presence of potassium cyanide. Aerobic growth of Escherichia coli with an oxidizable substrate as carbon source in the presence of low concentrations of KCN leads to the synthesis and integration into the membrane of menaquinone and cytochromes b558, a1 and d in addition to the redox carriers normally present under aerobic growth conditions, namely ubiquinone and cytochromes b562, b556 and o. The results are discussed with reference to other phenotypic and genotypic modifications to the electron-transport chains of E. coli."}
{"id": "PMID:1098671", "title": "The therapeutic activity of 1-(p-chlorobenzoyl)-5-methoxy-2-methylindole-3-acetic acid monohydrate glucosamide in rheumatoid arthritis (double blind trial).", "content": "A new nonsteroidal anti-inflammatory agent, 1-(p-chlorobenzyl)-5-methylindole-3-acetic acid monohydrate glucosamide (glucametacine, Teoremac\u00bf), was used in the treatment of a total of 39 patients including 31 cases of rheumatoid arthritis, 3 of ankylosing spondylitis, 1 of psoriatic rheumatism, 1 of chronic chondrocalcinosis and 3 of arthrosis. Teoremac was given for 14 days at a dosage of 420 mg/day. The drug was shown to be more or less active in all the patients except 4. The improvement in the various clinical parameters of rheumatoid arthritis studied was statistically significant. The side effects recorded were headache, which was severe in only 1 case, and \"minor\" disturbances of the gastro-intestinal tract. A double blind crossover trial was carried out in 15 patients with rheumatoid arthritis to compare the action of Teoremac (420 mg/day for 14 days) with that of indometacin (100 mg/day for 14 days). Under the conditions of the trial, the clinical findings showed Teoremac to be slightly superior to indometacin, but the difference was not significant statistically.", "contents": "The therapeutic activity of 1-(p-chlorobenzoyl)-5-methoxy-2-methylindole-3-acetic acid monohydrate glucosamide in rheumatoid arthritis (double blind trial). A new nonsteroidal anti-inflammatory agent, 1-(p-chlorobenzyl)-5-methylindole-3-acetic acid monohydrate glucosamide (glucametacine, Teoremac\u00bf), was used in the treatment of a total of 39 patients including 31 cases of rheumatoid arthritis, 3 of ankylosing spondylitis, 1 of psoriatic rheumatism, 1 of chronic chondrocalcinosis and 3 of arthrosis. Teoremac was given for 14 days at a dosage of 420 mg/day. The drug was shown to be more or less active in all the patients except 4. The improvement in the various clinical parameters of rheumatoid arthritis studied was statistically significant. The side effects recorded were headache, which was severe in only 1 case, and \"minor\" disturbances of the gastro-intestinal tract. A double blind crossover trial was carried out in 15 patients with rheumatoid arthritis to compare the action of Teoremac (420 mg/day for 14 days) with that of indometacin (100 mg/day for 14 days). Under the conditions of the trial, the clinical findings showed Teoremac to be slightly superior to indometacin, but the difference was not significant statistically."}
{"id": "PMID:1098672", "title": "[Insulin precursors--a historical synopsis. First diabetes treatment trials using pancreas extracts].", "content": "The highly interesting and successful attempts to treat diabetes with pancreas extracts deserve a historical review. Some of these experiments were performed long before the isolation and use of insulin by the Canadians Banting, Best, Macleod and Collip in 1921--22. Successful isolation of insulin, known as acomatol and pancreatin, was first carried out before the Canadian research-workers published their results. Particular credit goes above all to Paulesco, Reuter and Zuelzer.", "contents": "[Insulin precursors--a historical synopsis. First diabetes treatment trials using pancreas extracts]. The highly interesting and successful attempts to treat diabetes with pancreas extracts deserve a historical review. Some of these experiments were performed long before the isolation and use of insulin by the Canadians Banting, Best, Macleod and Collip in 1921--22. Successful isolation of insulin, known as acomatol and pancreatin, was first carried out before the Canadian research-workers published their results. Particular credit goes above all to Paulesco, Reuter and Zuelzer."}
{"id": "PMID:1098676", "title": "Experimental Shwartzman phenomenon in the heart muscle.", "content": "Intravenous injection of E. coli culture caused a sudden death in rabbits which were injected Streptococcus culture into the myocardium 24 h before the experiment. Pretreatment of animals with Trasylol-R, before injection of E. coli culture, caused an increase of survival time. In isolated Langendorff perfused heart from myocardially Streptococcus culture injected animals, E. coli culture filtrate caused a transitory increase and then a continuous decrease in coronary perfusion pressure together with an increase in heart rate. These findings are shown to be prevented by previous addition of Trasylol to the perfusion medium. According to these results it is speculated that a Shwartzman-like phenomenon can also occur in the heart muscle. The possible role of the activation of tissue kallikrein system in the production of this phenomenon is discussed.", "contents": "Experimental Shwartzman phenomenon in the heart muscle. Intravenous injection of E. coli culture caused a sudden death in rabbits which were injected Streptococcus culture into the myocardium 24 h before the experiment. Pretreatment of animals with Trasylol-R, before injection of E. coli culture, caused an increase of survival time. In isolated Langendorff perfused heart from myocardially Streptococcus culture injected animals, E. coli culture filtrate caused a transitory increase and then a continuous decrease in coronary perfusion pressure together with an increase in heart rate. These findings are shown to be prevented by previous addition of Trasylol to the perfusion medium. According to these results it is speculated that a Shwartzman-like phenomenon can also occur in the heart muscle. The possible role of the activation of tissue kallikrein system in the production of this phenomenon is discussed."}
{"id": "PMID:1098670", "title": "Septic arthritis in sickle-cell thalassemia. Pathophysiology of impaired response to infection.", "content": "The knee of a boy with sickle-cell thalassemia became infected with Salmonella enteritidis, sensitive to ampicillin. Doses of ampicillin that achieved anti-bacterial titers of 32 in synovial fluid failed to eradicate the organism. The synovial fluid exhibited severe hypoxia, hypercapnia, and lactic acidosis. Methods were developed to estimate the oxygen saturation of blood in regional venous capillaries, the degree of periarticular sickling, changes in the viscosity of local blood, and the articular ratio of blood flow to oxygen utilization. The results suggest that local vascular insufficiency associated with sickling may affect host response to infection and efficacy of antibiotics. The Salmonella C1 readily transformed to an L form on hypertonic medium, thus acquiring resistance to ampicillin and other cell-wall inhibitors.", "contents": "Septic arthritis in sickle-cell thalassemia. Pathophysiology of impaired response to infection. The knee of a boy with sickle-cell thalassemia became infected with Salmonella enteritidis, sensitive to ampicillin. Doses of ampicillin that achieved anti-bacterial titers of 32 in synovial fluid failed to eradicate the organism. The synovial fluid exhibited severe hypoxia, hypercapnia, and lactic acidosis. Methods were developed to estimate the oxygen saturation of blood in regional venous capillaries, the degree of periarticular sickling, changes in the viscosity of local blood, and the articular ratio of blood flow to oxygen utilization. The results suggest that local vascular insufficiency associated with sickling may affect host response to infection and efficacy of antibiotics. The Salmonella C1 readily transformed to an L form on hypertonic medium, thus acquiring resistance to ampicillin and other cell-wall inhibitors."}
{"id": "PMID:1098677", "title": "[Phalloidin antagonists. 2. Protective effect of disilybine in phalloidin poisoning of isolated hepatocytes].", "content": "Phalloidin produces marked protrusions on the surface of isolated hepatocytes prepared from rat livers. This typical in-vitro effect of the mushroom toxin is prevented or inhibited by preincubation with disilybin. The antagonistic effect depends on the concentration of disilybin and might be caused by an inhibition of phalloidin binding.", "contents": "[Phalloidin antagonists. 2. Protective effect of disilybine in phalloidin poisoning of isolated hepatocytes]. Phalloidin produces marked protrusions on the surface of isolated hepatocytes prepared from rat livers. This typical in-vitro effect of the mushroom toxin is prevented or inhibited by preincubation with disilybin. The antagonistic effect depends on the concentration of disilybin and might be caused by an inhibition of phalloidin binding."}
{"id": "PMID:1098678", "title": "Influence of D-penicillamine on the immune response of mice.", "content": "Studies on the influence of D-ss, ss-dimethylcystein (D-penicillamine, Metalcaptase-R) on the primary immune response of mice after immunization with sheep red blood cells show, that D-penicillamine up to doses of 20 mg/kg has no effect on the synthesis of hemagglutinating antibodies. Plaque forming cells in the spleen were not significantly reduced after administration of 80 mg/kg. A striking inhibition of the formation of immune rosettes is demonstrated. 80 mg/kg led to a significant reduction of rosette forming B-lymphocytes in the spleen.", "contents": "Influence of D-penicillamine on the immune response of mice. Studies on the influence of D-ss, ss-dimethylcystein (D-penicillamine, Metalcaptase-R) on the primary immune response of mice after immunization with sheep red blood cells show, that D-penicillamine up to doses of 20 mg/kg has no effect on the synthesis of hemagglutinating antibodies. Plaque forming cells in the spleen were not significantly reduced after administration of 80 mg/kg. A striking inhibition of the formation of immune rosettes is demonstrated. 80 mg/kg led to a significant reduction of rosette forming B-lymphocytes in the spleen."}
{"id": "PMID:1098683", "title": "Use of a dynamic method in calibration of dye-dilution curves during cardiac surgery.", "content": "A simplified dynamic method of calibration for dye-dilution curves is described. The method involves appropriate matching of the fluid volume in the mixing chamber, the blood withdrawal rate, and the calibration dose of dye. It was used to compare the observed versus calibrated pump output of a heart-lung machine with individual dye-dilution curves obtained from 15 patients during total cardiopulmonary bypass. The results indicate that the difference (range+14% to-8%) between the two is statistically insignificant. The method is rapid and relatively simple. It is particularly useful for cardiac output determination during unsteady clinical conditions because of the ability to calibrate dye curves at the time they are recorded.", "contents": "Use of a dynamic method in calibration of dye-dilution curves during cardiac surgery. A simplified dynamic method of calibration for dye-dilution curves is described. The method involves appropriate matching of the fluid volume in the mixing chamber, the blood withdrawal rate, and the calibration dose of dye. It was used to compare the observed versus calibrated pump output of a heart-lung machine with individual dye-dilution curves obtained from 15 patients during total cardiopulmonary bypass. The results indicate that the difference (range+14% to-8%) between the two is statistically insignificant. The method is rapid and relatively simple. It is particularly useful for cardiac output determination during unsteady clinical conditions because of the ability to calibrate dye curves at the time they are recorded."}
{"id": "PMID:1098684", "title": "Procainamide and phenytoin. Comparative study of their antiarrhythmic effects at apparent therapeutic plasma levels.", "content": "The antiarrhythmic effects of procainamide and phenytoin were studied in 81 patients admitted to the coronary care unit at the University Hospital in Link\u00f6ping because of a suspected or proven diagnosis of acute myocardial infarction, and who developed ventricular arrhyhmias, requiring treatment, during the first 8 hours in hospital. Patients were randomly allocated to a procainamide of phenytoin group. The drugs were given as intravenous and oral loading doses followed by oral maintenance therapy. Plasma levels of the two druge were frequently determined and the electrocardiogram was continuously recorded during the 24-hour trial and analysed minute by minute. A significantly higher frequency of therapeutic failure was found in the phenytoin group (23 of 35 patients)compared to the procainamide group(13 of 39 aptients) during the first 2 hours after initiation of therapy. Four patients in the phenytoin group and 2 in the procainamide group developed symptoms probably caused by the trial drugs, necessitating discontinuation of therapy. The mean plasma levels were usually within the apparent therapeutic range (for phenytoin 40-72 mumol/l (10-18 mug/ml), and for procainamide 17-34 mumol/l (4-8 mug/ml). Seventeen patients (68%) in the phenytoin group and 10 patients (48%) in the procainamide group had plasma concentrations within this range when the therapeutic failure was observed. Nine patients died in hospital but only one of them during the trial. The results of this investigation clearly demonstrate the overall superiority of procainamide over phenytoin as an antiarrhythmic drug in short-term therapy after acute myocardial infarction.", "contents": "Procainamide and phenytoin. Comparative study of their antiarrhythmic effects at apparent therapeutic plasma levels. The antiarrhythmic effects of procainamide and phenytoin were studied in 81 patients admitted to the coronary care unit at the University Hospital in Link\u00f6ping because of a suspected or proven diagnosis of acute myocardial infarction, and who developed ventricular arrhyhmias, requiring treatment, during the first 8 hours in hospital. Patients were randomly allocated to a procainamide of phenytoin group. The drugs were given as intravenous and oral loading doses followed by oral maintenance therapy. Plasma levels of the two druge were frequently determined and the electrocardiogram was continuously recorded during the 24-hour trial and analysed minute by minute. A significantly higher frequency of therapeutic failure was found in the phenytoin group (23 of 35 patients)compared to the procainamide group(13 of 39 aptients) during the first 2 hours after initiation of therapy. Four patients in the phenytoin group and 2 in the procainamide group developed symptoms probably caused by the trial drugs, necessitating discontinuation of therapy. The mean plasma levels were usually within the apparent therapeutic range (for phenytoin 40-72 mumol/l (10-18 mug/ml), and for procainamide 17-34 mumol/l (4-8 mug/ml). Seventeen patients (68%) in the phenytoin group and 10 patients (48%) in the procainamide group had plasma concentrations within this range when the therapeutic failure was observed. Nine patients died in hospital but only one of them during the trial. The results of this investigation clearly demonstrate the overall superiority of procainamide over phenytoin as an antiarrhythmic drug in short-term therapy after acute myocardial infarction."}
{"id": "PMID:1098680", "title": "Dose-response effect of single and combined clofibrate (Atromidin) and niceritrol (Perycit) treatment on serum lipids and lipoproteins in type II hyperlipoproteinaemia.", "content": "The dose-response effects of clofibrate and niceritrol on serum cholesterol and triglycerides (TG) were studied in 29 patients with Type IIa and IIb hyperlipoproteinaemia. In 17 patients, clofibrate and niceritrol were then given as combined treatment, and the effects of this combination on serum lipoproteins were studied. Clofibrate was given in three doses: 1.5,2 and 2.5 g/day. The optimum effect on serum TG and cholesterol in patients with Type IIa was observed with the 1.5 g dose and higher doses did not lower the serum lipids further. In patients with Type IIb the optimal clofibrate dose was 1.5-2 g/day. Niceritrol was then given in three doses: 3,45 and 6 g/day. In patients with Type IIa the serum TG concentration was not significantly affected by any dose used, whereas the reduction of the serum cholesterol concentration was dose dependent. In patients with Type IIb the reduction of both serum TG and cholesterol was dose dependent. Combined treatment with 2 g clofibrate and 3 g niceritrol resulted in a normal lipoprotein pattern i 15 out of 17 patients. The reduction of the serum lipids was approximately the same as during treatment with 6 g niceritrol/day. No additional side-effects were observed during combined treatment.", "contents": "Dose-response effect of single and combined clofibrate (Atromidin) and niceritrol (Perycit) treatment on serum lipids and lipoproteins in type II hyperlipoproteinaemia. The dose-response effects of clofibrate and niceritrol on serum cholesterol and triglycerides (TG) were studied in 29 patients with Type IIa and IIb hyperlipoproteinaemia. In 17 patients, clofibrate and niceritrol were then given as combined treatment, and the effects of this combination on serum lipoproteins were studied. Clofibrate was given in three doses: 1.5,2 and 2.5 g/day. The optimum effect on serum TG and cholesterol in patients with Type IIa was observed with the 1.5 g dose and higher doses did not lower the serum lipids further. In patients with Type IIb the optimal clofibrate dose was 1.5-2 g/day. Niceritrol was then given in three doses: 3,45 and 6 g/day. In patients with Type IIa the serum TG concentration was not significantly affected by any dose used, whereas the reduction of the serum cholesterol concentration was dose dependent. In patients with Type IIb the reduction of both serum TG and cholesterol was dose dependent. Combined treatment with 2 g clofibrate and 3 g niceritrol resulted in a normal lipoprotein pattern i 15 out of 17 patients. The reduction of the serum lipids was approximately the same as during treatment with 6 g niceritrol/day. No additional side-effects were observed during combined treatment."}
{"id": "PMID:1098687", "title": "The influence of treatment of the paralesional skin with dithranol on healing in psoriasis.", "content": "Application of dithranol confined to the normal skin surrounding thirty-six psoriatic lesions produced improvement in twenty-four lesions, which was significantly more than the improvement noted in four of thirty-six control lesions left untreated. Such healing was temporary and slower than in lesions treated conventionally. It is suggested that the apparently normal skin around a psoriatic lesion under treatment influences the psoriasis.", "contents": "The influence of treatment of the paralesional skin with dithranol on healing in psoriasis. Application of dithranol confined to the normal skin surrounding thirty-six psoriatic lesions produced improvement in twenty-four lesions, which was significantly more than the improvement noted in four of thirty-six control lesions left untreated. Such healing was temporary and slower than in lesions treated conventionally. It is suggested that the apparently normal skin around a psoriatic lesion under treatment influences the psoriasis."}
{"id": "PMID:1098688", "title": "5-Fluorouracil in the treatment of common warts of the hands. A double-blind study.", "content": "A double-blind study was carried out to compare the action of 5-fluorouracil and 10% salicylic acid varnish with that of a 10% salicylic acid varnish on common warts. Fifty per cent of the lesions disappeared with the combined preparation and only 4% with the varnish containing salicylic acid alone. The presence of warts on the hands of 45.8% of the female workers of a fowl slaughterhouse is described.", "contents": "5-Fluorouracil in the treatment of common warts of the hands. A double-blind study. A double-blind study was carried out to compare the action of 5-fluorouracil and 10% salicylic acid varnish with that of a 10% salicylic acid varnish on common warts. Fifty per cent of the lesions disappeared with the combined preparation and only 4% with the varnish containing salicylic acid alone. The presence of warts on the hands of 45.8% of the female workers of a fowl slaughterhouse is described."}
{"id": "PMID:1098689", "title": "A controlled trial on the use of topical 5-fluorouracil on viral warts.", "content": "A double-blind controlled trial was performed on the use of topical 5% 5-fluorouracil ointment as a treatment for viral warts. The results showed the 5-fluorouracil to be better than the placebo.", "contents": "A controlled trial on the use of topical 5-fluorouracil on viral warts. A double-blind controlled trial was performed on the use of topical 5% 5-fluorouracil ointment as a treatment for viral warts. The results showed the 5-fluorouracil to be better than the placebo."}
{"id": "PMID:1098691", "title": "Photoaffinity labeling of the ribosomal A site with S-(p-azidophenacyl)valyl-tRNA.", "content": "S-(p-Azidophenacyl)valyl-tRNA, an analog of valyl-tRNA which has a photoaffinity label attached to its 4-thiouridine residue, was bound to the ribosomal A site at 10 mM Mg2+. Binding was stimulated 25-fold by the presence of elongation factor EFTu. Photoactivation of the p-azidophenacyl group by irradiation resulted in covalent linking of 6% of the noncovalently bound tRNA to the ribosomes. Covalent linking was dependent on the simultaneous presence of ribosomes, poly(U2,G),EFTu.GTP, required irradiation, and did not occur when S-(phenacyl)valyl-tRNA, a nonphotolyzable analog, replaced S-(p-azidophenacyl)valyl-tRNA. The attached tRNA was distributed approximately equally between both the 30S and 50S subunits. At the 30S subunit, 30% of the tRNA was bound to protein while 70% was linked to 16S RNA. At the 50S subunit, however, negligible binding to the 23S RNA was observed. More than 90% of the tRNA was attached to low molecular weight material according to sodium dodecyl sulfate-sucrose gradient analysis, and more than 87% of this fraction consisted of tRNA-protein complexes as assayed by phenol solubility and electrophoretic mobility before and after protease treatment. These results, in conjunction with our previous report (I. Schwartz and J Ofengand (1974), Proc. Natl. Acad. Sci. U.S.A. 71, 3951) which showed that covalent linking of this same tRNA derivative at the ribosomal P site resulted in attachment solely to the 16S RNA, demonstrate that 16S, but not 23S or 5S rRNA, is an important component of the tRNA binding site in the region of the 4-thiouridine residue and furthermore show that ribosomal A and P sites are topologically distinct.", "contents": "Photoaffinity labeling of the ribosomal A site with S-(p-azidophenacyl)valyl-tRNA. S-(p-Azidophenacyl)valyl-tRNA, an analog of valyl-tRNA which has a photoaffinity label attached to its 4-thiouridine residue, was bound to the ribosomal A site at 10 mM Mg2+. Binding was stimulated 25-fold by the presence of elongation factor EFTu. Photoactivation of the p-azidophenacyl group by irradiation resulted in covalent linking of 6% of the noncovalently bound tRNA to the ribosomes. Covalent linking was dependent on the simultaneous presence of ribosomes, poly(U2,G),EFTu.GTP, required irradiation, and did not occur when S-(phenacyl)valyl-tRNA, a nonphotolyzable analog, replaced S-(p-azidophenacyl)valyl-tRNA. The attached tRNA was distributed approximately equally between both the 30S and 50S subunits. At the 30S subunit, 30% of the tRNA was bound to protein while 70% was linked to 16S RNA. At the 50S subunit, however, negligible binding to the 23S RNA was observed. More than 90% of the tRNA was attached to low molecular weight material according to sodium dodecyl sulfate-sucrose gradient analysis, and more than 87% of this fraction consisted of tRNA-protein complexes as assayed by phenol solubility and electrophoretic mobility before and after protease treatment. These results, in conjunction with our previous report (I. Schwartz and J Ofengand (1974), Proc. Natl. Acad. Sci. U.S.A. 71, 3951) which showed that covalent linking of this same tRNA derivative at the ribosomal P site resulted in attachment solely to the 16S RNA, demonstrate that 16S, but not 23S or 5S rRNA, is an important component of the tRNA binding site in the region of the 4-thiouridine residue and furthermore show that ribosomal A and P sites are topologically distinct."}
{"id": "PMID:1098692", "title": "Purification and properties of nucleic acids from an unusual cytoplasmic organelle in the flagellate protozoan Crithidia oncopelti.", "content": "A simple, rapid method for preparing bipolar bodies from sonicated (rithidia oncopelti cells, with a yield of 2-5%, is described. Apart from 2-4% contamination with unbroken cells the fraction was considered pure with respect to contamination by other nucleic acid-containing organelles, as judged by light and electron microscopy. A light satellite DNA, f bouyant density 1.695 g/ml in neutral CsCl, and derived from the bipolar body, had a Tm of 81.6 degrees C in0.15 M NaCl/0.015 M sodium citrate (pH 7.0) and a kinetic complexity of 2.7 with 109. The bipolar body fraction also contained ribonucleoprotein particles with and s20,w of 67 S, in contrast to cytoplasmic ribosomes (87 S). Bipolar body ribosomes contained rRNA components which migraged coincidentally with Escherichia coli rRNA (molecular weights 1.07 with 10-6 and 0.56 with 10-6) on polyacrylamide gel electrophoresis. Cytoplasmic ribosomes contained rRNAs of molecular weights 1.30 with 10-6 and 0.83 with 10-6. Bipolar body rRNA accounted for up to 10% of the rRNA extracted from cells. The properties of these bipolar body nucleic acids provide good evidence for the bacterial nature of this subcellular component.", "contents": "Purification and properties of nucleic acids from an unusual cytoplasmic organelle in the flagellate protozoan Crithidia oncopelti. A simple, rapid method for preparing bipolar bodies from sonicated (rithidia oncopelti cells, with a yield of 2-5%, is described. Apart from 2-4% contamination with unbroken cells the fraction was considered pure with respect to contamination by other nucleic acid-containing organelles, as judged by light and electron microscopy. A light satellite DNA, f bouyant density 1.695 g/ml in neutral CsCl, and derived from the bipolar body, had a Tm of 81.6 degrees C in0.15 M NaCl/0.015 M sodium citrate (pH 7.0) and a kinetic complexity of 2.7 with 109. The bipolar body fraction also contained ribonucleoprotein particles with and s20,w of 67 S, in contrast to cytoplasmic ribosomes (87 S). Bipolar body ribosomes contained rRNA components which migraged coincidentally with Escherichia coli rRNA (molecular weights 1.07 with 10-6 and 0.56 with 10-6) on polyacrylamide gel electrophoresis. Cytoplasmic ribosomes contained rRNAs of molecular weights 1.30 with 10-6 and 0.83 with 10-6. Bipolar body rRNA accounted for up to 10% of the rRNA extracted from cells. The properties of these bipolar body nucleic acids provide good evidence for the bacterial nature of this subcellular component."}
{"id": "PMID:1098693", "title": "Photoinduced convalent crosslinkage, in situ, of Escherichia coli 50 S ribosomal proteins to rRNA.", "content": "Irradiation of aqueous solutions of Escherichia coli 50 S ribosomal subunits with 253.7 nm light results in the covalent crosslinkage of the rRNA and protein components. Neither peptide bond cleavage nor protein-protein crosslinkage accompanies the crosslinkage reactionmin addition, substantial photoinduced modifications in the primary structure of the ribosomal proteins, other than crosslinkage to rRNA, are not detected. The crosslinkage of the ribosomal proteins to the rRNA proroceeds in two discrete, dose-dependent steps. The first step, requires an input of up to 3 with 10-20 Quanta of 253.7nm radiation, and results in the crosslinkage of less than half of the ribosomal proteins to the rRNA. The second step requires an input of greater than 3 with 10-20 Quanta of 253.7 nm radiation, and results in the crosslinkage of the remaining ribosomal proteins to the rRNA. The possible relationship of the nature of the corsslinkage reaction to the spatial orientations of the rRNA and protein molecules in the intact 50 S ribosomal subunits is discussed.", "contents": "Photoinduced convalent crosslinkage, in situ, of Escherichia coli 50 S ribosomal proteins to rRNA. Irradiation of aqueous solutions of Escherichia coli 50 S ribosomal subunits with 253.7 nm light results in the covalent crosslinkage of the rRNA and protein components. Neither peptide bond cleavage nor protein-protein crosslinkage accompanies the crosslinkage reactionmin addition, substantial photoinduced modifications in the primary structure of the ribosomal proteins, other than crosslinkage to rRNA, are not detected. The crosslinkage of the ribosomal proteins to the rRNA proroceeds in two discrete, dose-dependent steps. The first step, requires an input of up to 3 with 10-20 Quanta of 253.7nm radiation, and results in the crosslinkage of less than half of the ribosomal proteins to the rRNA. The second step requires an input of greater than 3 with 10-20 Quanta of 253.7 nm radiation, and results in the crosslinkage of the remaining ribosomal proteins to the rRNA. The possible relationship of the nature of the corsslinkage reaction to the spatial orientations of the rRNA and protein molecules in the intact 50 S ribosomal subunits is discussed."}
{"id": "PMID:1098694", "title": "The characterization of energized and partially de-energized (respiration-independent) beta-galactoside transport into Escherichia coli.", "content": "Unidirectional fluxes of [14C]lactose by whole cells of Escherichia coli under highly energized and partially de-energized (in the presence of CN-) conditions are analyzed kinetically. When the cells are energized, the value for V influx is 0.45 +/- 0.01 mM internal concentration increment/s and Kt is 0.26 +/- 0.03 mM. At an external concentration of 0.61 mM the steady-state internal concentration is 0.25 M, reached after about 1h. The maximum steady-state concentration ratio is 2-10(3). The efflux process under these conditions is non-saturable, being linearly dependent upon internal concentration over the range 25-250 mM with a first-order rate constant of 8.8 +/- 0.2-10(-4) S-1. The transport in the presence of CN- is active, with a maximum concentration ratio (internal concentration/external concentration) of 104, and the uptake is mimicked by anoxia (less than 70 ppm O2). The effects of CN- are to lower the V for influx and to change the efflux from a non-saturable to a saturable process with a value for Kt (60 mM) intermediate between that for energized efflux (greater than 250 mM) and influx (0.3-0.6 mM), the latter value not changing appreciably. Partial de-energization thus affects both the influx and efflux processes.", "contents": "The characterization of energized and partially de-energized (respiration-independent) beta-galactoside transport into Escherichia coli. Unidirectional fluxes of [14C]lactose by whole cells of Escherichia coli under highly energized and partially de-energized (in the presence of CN-) conditions are analyzed kinetically. When the cells are energized, the value for V influx is 0.45 +/- 0.01 mM internal concentration increment/s and Kt is 0.26 +/- 0.03 mM. At an external concentration of 0.61 mM the steady-state internal concentration is 0.25 M, reached after about 1h. The maximum steady-state concentration ratio is 2-10(3). The efflux process under these conditions is non-saturable, being linearly dependent upon internal concentration over the range 25-250 mM with a first-order rate constant of 8.8 +/- 0.2-10(-4) S-1. The transport in the presence of CN- is active, with a maximum concentration ratio (internal concentration/external concentration) of 104, and the uptake is mimicked by anoxia (less than 70 ppm O2). The effects of CN- are to lower the V for influx and to change the efflux from a non-saturable to a saturable process with a value for Kt (60 mM) intermediate between that for energized efflux (greater than 250 mM) and influx (0.3-0.6 mM), the latter value not changing appreciably. Partial de-energization thus affects both the influx and efflux processes."}
{"id": "PMID:1098695", "title": "The effects of organic solvents on Escherichia coli DNA polymerase III.", "content": "The polymerizing ability of Escherichia coli DNA polymerase III is enhanced by a variety of water-miscible organic solvents of which dimethyl sulfoxide at 17% (v/v) is the most effective tested. The extent of stimulation depends on the organic solvent used and its concentration, but shows no obvious correlation with the chemical structure of the solvent or its dielectric constant. Kinetic studies indicate that the mechanism of stimulation is complex.", "contents": "The effects of organic solvents on Escherichia coli DNA polymerase III. The polymerizing ability of Escherichia coli DNA polymerase III is enhanced by a variety of water-miscible organic solvents of which dimethyl sulfoxide at 17% (v/v) is the most effective tested. The extent of stimulation depends on the organic solvent used and its concentration, but shows no obvious correlation with the chemical structure of the solvent or its dielectric constant. Kinetic studies indicate that the mechanism of stimulation is complex."}
{"id": "PMID:1098696", "title": "Degradation of double-stranded RNA by a monomeric derivative of ribonuclease BS-1.", "content": "Double-stranded RNA, resistant to the action of pancreatic monomeric RNAase A, is actively degraded by seminal dimeric RNAase BS-1. Evidence is presented that a monomeric derivative of seminal RNAase degrades double-stranded RNA as efficiently as the parent dimeric molecule. This finding is discussed in the light of the hypothesis previously advanced that two active sites simultaneously available on an enzyme molecule may be responsible for degradation of double-stranded polyribonucleotides.", "contents": "Degradation of double-stranded RNA by a monomeric derivative of ribonuclease BS-1. Double-stranded RNA, resistant to the action of pancreatic monomeric RNAase A, is actively degraded by seminal dimeric RNAase BS-1. Evidence is presented that a monomeric derivative of seminal RNAase degrades double-stranded RNA as efficiently as the parent dimeric molecule. This finding is discussed in the light of the hypothesis previously advanced that two active sites simultaneously available on an enzyme molecule may be responsible for degradation of double-stranded polyribonucleotides."}
{"id": "PMID:1098697", "title": "Isolation and properties of the transcription complex of Escherichia coli RNA polymerase.", "content": "A procedure is described which permits complete separation of a transcription complex formed with template DNA, growing RNA chain and functioning RNA polymerase, from RNA polymerase molecules which have bound to DNA but not initiated RNA synthesis. The method is based on the marked stability of the transcription complex to dissociation by high concentrations of CsCl or CS2SO4 which enable banding the complex after equilibrium centrifugation. With use of the newly developed procedure, affinity of Escherichia coli RNA polymerase to T7 phage DNA was found to increase during initiation of RNA synthesis but then decrease concomitant with elongation of RNA chain presumably due to migration of the enzyme to DNA sites of weak affinity. Under the conditions of maximum affinity, the transcription complex contained one core polymerase for each T7 DNA if it was isolated by centrifugation in CsCl; in contrast, 2-6 enzyme molecules remained attached on the complex when the centrifugation was carried out in Cs2SO4. Thus, RNA polymerases bound to different sites of transcription initiation appear to be distinguished based on the affinity of interaction. Attempts are also described to isolate the transcription complex in vivo by Cs2SO4 centrifugation by Brij 58-deoxycholate lysate of lysozyme-treated Escherichia coli cells. The isolated complex contained approx. 50 polymerase molecules per Escherichia coli genome as well as other unidentified proteins.", "contents": "Isolation and properties of the transcription complex of Escherichia coli RNA polymerase. A procedure is described which permits complete separation of a transcription complex formed with template DNA, growing RNA chain and functioning RNA polymerase, from RNA polymerase molecules which have bound to DNA but not initiated RNA synthesis. The method is based on the marked stability of the transcription complex to dissociation by high concentrations of CsCl or CS2SO4 which enable banding the complex after equilibrium centrifugation. With use of the newly developed procedure, affinity of Escherichia coli RNA polymerase to T7 phage DNA was found to increase during initiation of RNA synthesis but then decrease concomitant with elongation of RNA chain presumably due to migration of the enzyme to DNA sites of weak affinity. Under the conditions of maximum affinity, the transcription complex contained one core polymerase for each T7 DNA if it was isolated by centrifugation in CsCl; in contrast, 2-6 enzyme molecules remained attached on the complex when the centrifugation was carried out in Cs2SO4. Thus, RNA polymerases bound to different sites of transcription initiation appear to be distinguished based on the affinity of interaction. Attempts are also described to isolate the transcription complex in vivo by Cs2SO4 centrifugation by Brij 58-deoxycholate lysate of lysozyme-treated Escherichia coli cells. The isolated complex contained approx. 50 polymerase molecules per Escherichia coli genome as well as other unidentified proteins."}
{"id": "PMID:1098703", "title": "Perspectives on acute enteric disease epidemiology and control.", "content": "This paper reviews the major epidemiologic features of the acute enteric diseases and outlines a plan for their prevention and control in developing countries. Annual mortality from enteric diseases ranges from 10 per 1000,000 in highly developed countries to as much as 500 per 100,000 in developing countries. Most agents of enteric disease are spread by one of two routes: direct person-to-person contact or ingestion of contaminated vehicles (food or water). An effective control program should therefore focus on common epidemiologic factors, rather than on agent-specific remedies such as vaccines or antibiotics. The major operational components of such a program should include oral fluid replacement therapy, improvements in environmental sanitation, health education to promote personal hygiene and proper food handling, and epidemiologic surveillance to monitor public health needs and evaluate the impact of health measures.", "contents": "Perspectives on acute enteric disease epidemiology and control. This paper reviews the major epidemiologic features of the acute enteric diseases and outlines a plan for their prevention and control in developing countries. Annual mortality from enteric diseases ranges from 10 per 1000,000 in highly developed countries to as much as 500 per 100,000 in developing countries. Most agents of enteric disease are spread by one of two routes: direct person-to-person contact or ingestion of contaminated vehicles (food or water). An effective control program should therefore focus on common epidemiologic factors, rather than on agent-specific remedies such as vaccines or antibiotics. The major operational components of such a program should include oral fluid replacement therapy, improvements in environmental sanitation, health education to promote personal hygiene and proper food handling, and epidemiologic surveillance to monitor public health needs and evaluate the impact of health measures."}
{"id": "PMID:1098708", "title": "The use of Opsite, a vapour permeable dressing, on skin graft donor sites.", "content": "A trial of a moisture vapour permeable dressing (Opsite) on split-skin graft donor sites of 53 patients has been carried out. The main advantages were freedom from pain, early healing and lack of bulky dressings. The disadvantages were post-operative leakage of fluid from under the Opsite and fragility of the newly healed donor site for the first few days. An occlusive dressing for the first 48 hours post-operatively is suggested to help overcome the leakage problem, and care of the healed donor site, with particular emphasis on not removing Opsite that is adherent to it, is stressed.", "contents": "The use of Opsite, a vapour permeable dressing, on skin graft donor sites. A trial of a moisture vapour permeable dressing (Opsite) on split-skin graft donor sites of 53 patients has been carried out. The main advantages were freedom from pain, early healing and lack of bulky dressings. The disadvantages were post-operative leakage of fluid from under the Opsite and fragility of the newly healed donor site for the first few days. An occlusive dressing for the first 48 hours post-operatively is suggested to help overcome the leakage problem, and care of the healed donor site, with particular emphasis on not removing Opsite that is adherent to it, is stressed."}
{"id": "PMID:1098709", "title": "Burli (mycobacterial) ulcers in Caucasians in Nigeria.", "content": "Seven Caucasians with Buruli ulcers have been treated. The disease did not behave differently in them from its behaviour in Africans nor has liability to infection any racial basis. Surgical removal with or without skin grafting is the treatment of choice.", "contents": "Burli (mycobacterial) ulcers in Caucasians in Nigeria. Seven Caucasians with Buruli ulcers have been treated. The disease did not behave differently in them from its behaviour in Africans nor has liability to infection any racial basis. Surgical removal with or without skin grafting is the treatment of choice."}
{"id": "PMID:1098711", "title": "The length: breadth ratio of skin flaps on the trunk: two new long narrow flaps.", "content": "Our experience of skin flaps on the trunk is reviewed. A single-pedicle flap based on the sterno-clavicular region and a bipedicled longitudinal back flap have length: breadth ratios much in excess of random pattern flaps.", "contents": "The length: breadth ratio of skin flaps on the trunk: two new long narrow flaps. Our experience of skin flaps on the trunk is reviewed. A single-pedicle flap based on the sterno-clavicular region and a bipedicled longitudinal back flap have length: breadth ratios much in excess of random pattern flaps."}
{"id": "PMID:1098705", "title": "[Allergen-induced bronchoconstriction in asthma: antagonistic effect of a synthetic anticholinergic drug (author's transl)].", "content": "In ten allergic patients, the effect of inhaltion of increasing quantities of grass pollen on specific airways resistance (SRaw, measured by plethysmography) was estimated after preliminary inhalation of a placebo and of a synthetic anticholinergic drug (SCH 10000). After placebo, the inhalation of grass pollen induced in all cases a bronchoconstriction (mean increase of SRaw : 170 %). After SCH 1000, and for the same quantity of pollen inhaled, seven patients were entirely protected against the bronchoconstrictor effect of the allergen ; for the whole group, the observed bronchoconstriction was significantly lower (p less than 0.01, mean increase of SRaw : 60 %). The possible mechanisms of this protection are discussed. The results suggest that SCH 1000 could be used as a preventive drug in pollinic asthma.", "contents": "[Allergen-induced bronchoconstriction in asthma: antagonistic effect of a synthetic anticholinergic drug (author's transl)]. In ten allergic patients, the effect of inhaltion of increasing quantities of grass pollen on specific airways resistance (SRaw, measured by plethysmography) was estimated after preliminary inhalation of a placebo and of a synthetic anticholinergic drug (SCH 10000). After placebo, the inhalation of grass pollen induced in all cases a bronchoconstriction (mean increase of SRaw : 170 %). After SCH 1000, and for the same quantity of pollen inhaled, seven patients were entirely protected against the bronchoconstrictor effect of the allergen ; for the whole group, the observed bronchoconstriction was significantly lower (p less than 0.01, mean increase of SRaw : 60 %). The possible mechanisms of this protection are discussed. The results suggest that SCH 1000 could be used as a preventive drug in pollinic asthma."}
{"id": "PMID:1098712", "title": "Hypospadias repair using polyglycolic acid sutures.", "content": "A two-stage hypospadias repair is described and the results of 50 consecutive cases are reported in which polyglycolic acid sutures were used.", "contents": "Hypospadias repair using polyglycolic acid sutures. A two-stage hypospadias repair is described and the results of 50 consecutive cases are reported in which polyglycolic acid sutures were used."}
{"id": "PMID:1098713", "title": "Aggressive non-irradiated basal cell carcinoma of forehead treated by free flap transfer.", "content": "A case of recurrent basal cell carcinoma resistant to repeated excision is reported. The forehead defect was covered with a deltopectoral free flap transfer in which 1 artery and 2 veins were anastomosed.", "contents": "Aggressive non-irradiated basal cell carcinoma of forehead treated by free flap transfer. A case of recurrent basal cell carcinoma resistant to repeated excision is reported. The forehead defect was covered with a deltopectoral free flap transfer in which 1 artery and 2 veins were anastomosed."}
{"id": "PMID:1098720", "title": "Gonadotrophin-releasing hormone treatment for induction of follicular maturation and ovulation in amenorrhoeic women with anorexia nervosa.", "content": "Follicular maturation and ovulation can be induced in amenorrhoeic women with anorexia nervosa by long-term treatment with 500 mug of luteinizing hormone releasing hormone (LH-RH) every eight hours. In some women, however, treatment with LH-RH alone results in ovulatory menstrual cycles with indications of luteal phase insufficiency. Human chorionic gonadotrophin (HCG) was therefore given with LH-RH during three treatment cycles. This resulted in ovulation and normal corpus-luteum function, as shown by the occurrence of a single pregnancy in the only involuntarily sterile patient. During the prolonged LH-RH treatment the LH response to LH-RH increased in parallel with the increased oestrogen secretion while the follicle-stimulating hormone response to LH-RH decreased. These changes in the pituitary responsiveness to LH-RH may result from modulating effects on the pituitary by the sex steroids.", "contents": "Gonadotrophin-releasing hormone treatment for induction of follicular maturation and ovulation in amenorrhoeic women with anorexia nervosa. Follicular maturation and ovulation can be induced in amenorrhoeic women with anorexia nervosa by long-term treatment with 500 mug of luteinizing hormone releasing hormone (LH-RH) every eight hours. In some women, however, treatment with LH-RH alone results in ovulatory menstrual cycles with indications of luteal phase insufficiency. Human chorionic gonadotrophin (HCG) was therefore given with LH-RH during three treatment cycles. This resulted in ovulation and normal corpus-luteum function, as shown by the occurrence of a single pregnancy in the only involuntarily sterile patient. During the prolonged LH-RH treatment the LH response to LH-RH increased in parallel with the increased oestrogen secretion while the follicle-stimulating hormone response to LH-RH decreased. These changes in the pituitary responsiveness to LH-RH may result from modulating effects on the pituitary by the sex steroids."}
{"id": "PMID:1098721", "title": "Value of prednisone in combination chemotherapy of stage IV Hodgkin's disease. Report from the British National Lymphoma Investigation.", "content": "A combination of mustine, vincristine (Oncovin), procarbazine, and prednisione (MOPP) and the same combination without prednisone (MOP) were compared in the treatment of stage IV Hodgkin's disease in a prospective randomized trial. The complete remission rates were 80% with MOPP and 44% with MOP; the difference was highly significant. Prednisone seems to be an important component of the MOPP combination in the management of stage IV Hodgkin's disease.", "contents": "Value of prednisone in combination chemotherapy of stage IV Hodgkin's disease. Report from the British National Lymphoma Investigation. A combination of mustine, vincristine (Oncovin), procarbazine, and prednisione (MOPP) and the same combination without prednisone (MOP) were compared in the treatment of stage IV Hodgkin's disease in a prospective randomized trial. The complete remission rates were 80% with MOPP and 44% with MOP; the difference was highly significant. Prednisone seems to be an important component of the MOPP combination in the management of stage IV Hodgkin's disease."}
{"id": "PMID:1098726", "title": "Failure of low-dose heparin to improve efficacy of peroperative intermittent calf compression in preventing postoperative deep vein thrombosis.", "content": "The possible benefits of adding a low-dose heparin regimen to the technique of peroperative intermittent calf compression for preventing deep vein thrombosis (D.V.T.) were assessed in a randomized trial in 84 surgical patients. The efficacy of peroperative intermittent calf compression was not enhanced by a low-dose heparin regimen, but neither was it worsened. Age, weight, duration, operation, and malignant disease did not affect the relative effectiveness of the two regimens of prophylaxis. The results confirmed that venous stasis is the principal cause of D.V.T.", "contents": "Failure of low-dose heparin to improve efficacy of peroperative intermittent calf compression in preventing postoperative deep vein thrombosis. The possible benefits of adding a low-dose heparin regimen to the technique of peroperative intermittent calf compression for preventing deep vein thrombosis (D.V.T.) were assessed in a randomized trial in 84 surgical patients. The efficacy of peroperative intermittent calf compression was not enhanced by a low-dose heparin regimen, but neither was it worsened. Age, weight, duration, operation, and malignant disease did not affect the relative effectiveness of the two regimens of prophylaxis. The results confirmed that venous stasis is the principal cause of D.V.T."}
{"id": "PMID:1098727", "title": "Immunopotentiation with levamisole in resectable bronchogenic carcinoma: a double-blind controlled trial; Study Group for Bronchogenic Carcinoma.", "content": "A long-term multicentre double-blind study was designed to test the immunotropic effects of levamisole in patients undergoing operation for primary bronchial carcinoma. They received levamisole 50 mg three times a day by mouth or placebo for three days every fortnight, starting three days before surgery. Unless there was clinical evidence of recurrence, cytostatic drugs, corticosteroids, and radiotherapy were prohibited. In the 111 patients who have been followed up for one year the incidence of side effects was similar in both groups. Recurrences occurred in 10 out of 51 patients (seven deaths) receiving levamisole and in 20 out of 60 (12 deaths) receiving placebo. Further analysis showed that there were fewer recurrences on levamisole in patients with squamous cell carcinomas and medium and large primary tumours and fewer suspected and proved recurrences and deaths from metastases on levamisole in patients with extended tumours. Distant recurrences tended to be less common with levamisole, whereas the disease-free interval in relapsing patients was almost identical in the two groups. These interim results show that levamisole seems to exert its beneficial effect by preventing immunosuppression due to surgery.", "contents": "Immunopotentiation with levamisole in resectable bronchogenic carcinoma: a double-blind controlled trial; Study Group for Bronchogenic Carcinoma. A long-term multicentre double-blind study was designed to test the immunotropic effects of levamisole in patients undergoing operation for primary bronchial carcinoma. They received levamisole 50 mg three times a day by mouth or placebo for three days every fortnight, starting three days before surgery. Unless there was clinical evidence of recurrence, cytostatic drugs, corticosteroids, and radiotherapy were prohibited. In the 111 patients who have been followed up for one year the incidence of side effects was similar in both groups. Recurrences occurred in 10 out of 51 patients (seven deaths) receiving levamisole and in 20 out of 60 (12 deaths) receiving placebo. Further analysis showed that there were fewer recurrences on levamisole in patients with squamous cell carcinomas and medium and large primary tumours and fewer suspected and proved recurrences and deaths from metastases on levamisole in patients with extended tumours. Distant recurrences tended to be less common with levamisole, whereas the disease-free interval in relapsing patients was almost identical in the two groups. These interim results show that levamisole seems to exert its beneficial effect by preventing immunosuppression due to surgery."}
{"id": "PMID:1098730", "title": "Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.", "content": "The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis.", "contents": "Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis. The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis."}
{"id": "PMID:1098731", "title": "Automation of a flocculation test for syphilis on Groupamatic equipment.", "content": "A flocculation reaction employing a cardiolipid antigen was used for syphilis screening on Groupamatic equipment in parallel with conventional screening reactions: Kolmer CF, RPCF, Kahn, Kline, and RPR. The positive samples were confirmed by FTA-200, FTA-ABS, TPI, and in some cases by TPHA. There were 5,212 known samples which had already been tested by all methods and of which 1,648 were positive, and 58,636 screened samples including 65 positives. Half of the samples in the first series were taken without anticoagulant; the remainder were collected in potassium EDTA. The percentage of false positives with the Groupamatic was about 1-4 per cent. The percentage of false negatives among positve (greater than or equal+) samples varied from 0-18 to 1-3 per cent.; on the other hand the sensitivity was less good for samples giving doubtful and/or dissociated reactions in conventional screening reactions. The specificity and sensitivity of this technique are acceptable for a blood transfusion centre. The reproducibility is excellent and the automatic reading of results accurate. Additional advantages are rapidity (340 samples processed per hour); simultaneous performance of eleven other immunohaematological reactions; no contamination between samples; automatic reading, interpretation, and print-out of results; and saving of time because samples are not filed sequentially and are automatically identified when the results are obtained. Although the importance of syphilis in blood transfusion seems small, estimates of the risk are difficult and further investigations are planned.", "contents": "Automation of a flocculation test for syphilis on Groupamatic equipment. A flocculation reaction employing a cardiolipid antigen was used for syphilis screening on Groupamatic equipment in parallel with conventional screening reactions: Kolmer CF, RPCF, Kahn, Kline, and RPR. The positive samples were confirmed by FTA-200, FTA-ABS, TPI, and in some cases by TPHA. There were 5,212 known samples which had already been tested by all methods and of which 1,648 were positive, and 58,636 screened samples including 65 positives. Half of the samples in the first series were taken without anticoagulant; the remainder were collected in potassium EDTA. The percentage of false positives with the Groupamatic was about 1-4 per cent. The percentage of false negatives among positve (greater than or equal+) samples varied from 0-18 to 1-3 per cent.; on the other hand the sensitivity was less good for samples giving doubtful and/or dissociated reactions in conventional screening reactions. The specificity and sensitivity of this technique are acceptable for a blood transfusion centre. The reproducibility is excellent and the automatic reading of results accurate. Additional advantages are rapidity (340 samples processed per hour); simultaneous performance of eleven other immunohaematological reactions; no contamination between samples; automatic reading, interpretation, and print-out of results; and saving of time because samples are not filed sequentially and are automatically identified when the results are obtained. Although the importance of syphilis in blood transfusion seems small, estimates of the risk are difficult and further investigations are planned."}
{"id": "PMID:1098732", "title": "Treatment of trichomoniasis in the female. A comparison of metronidazole and nimorazole.", "content": "The effectiveness of metronidazole (Flagyl) and nimorazole (Naxogin) has been compared by using these drugs in the recommended dosage in alternate patients in a series of 218 consecutive cases of vaginal trichomoniasis. Follow-up tests in 100 patients treated with metronidazole and 97 treated with nimorazole indicated cure-rates of 95 and 82 per cent. respectively. Male consorts were examined and given treatment on epidemiological grounds in about 70 per cent. of both treatment groups. Both drugs were free from significant side-effects. The causes of treatment failure in trichomoniasis are discussed and the desirability of relating dosage to the patient's body-weight is suggested. This factor may be especially important in deciding the dosage given on epidemiological grounds to the male consorts. It may also be an important advantage in the current trend of treating trichomoniasis in both sexes with larger doses over a much shorter time.", "contents": "Treatment of trichomoniasis in the female. A comparison of metronidazole and nimorazole. The effectiveness of metronidazole (Flagyl) and nimorazole (Naxogin) has been compared by using these drugs in the recommended dosage in alternate patients in a series of 218 consecutive cases of vaginal trichomoniasis. Follow-up tests in 100 patients treated with metronidazole and 97 treated with nimorazole indicated cure-rates of 95 and 82 per cent. respectively. Male consorts were examined and given treatment on epidemiological grounds in about 70 per cent. of both treatment groups. Both drugs were free from significant side-effects. The causes of treatment failure in trichomoniasis are discussed and the desirability of relating dosage to the patient's body-weight is suggested. This factor may be especially important in deciding the dosage given on epidemiological grounds to the male consorts. It may also be an important advantage in the current trend of treating trichomoniasis in both sexes with larger doses over a much shorter time."}
{"id": "PMID:1098734", "title": "Electron microscopy of experimental axonal degeneration in photochemically modified Golgi preparations: a procedure for precise mapping of nervous connections.", "content": "A new version is presented of a procedure for bleaching (deimpregnating) silver chromate Golgi preparations through ultraviolet (UV) light irradiation followed by thiosulphate treatment, leaving only scattered particles of metallic silver. This version gives a better ultrastructure of the tissue than obtained before. Moreover, the results of studying a specific connection in such material are reported. The perforant path, connecting the entorhinal area with the hippocampus and fascia dentata, was transected in the rat and after a 2-day survival the brain was subjected to Golgi impregnation followed by the deimpregnation procedure. Blocks of tissue containing impregnated fascia dentata granule cell dendrites were selected for electron microscopy. Postsynaptic thickenings associated with degenerated boutons could be discerned within the deimpregnated dendrites, proving that the entorhino-dentate fibres end on the dendrites of the granule cells (smooth surfaces as well as spines) and that they end at asymmetric junctions (of Gray's type 1). While this conclusion was as expected from previous experimental studies carried out with different methods it is thought that the same strategy for cell-to-cell mapping of nervous connections may be used also in other parts of the central nervous system.", "contents": "Electron microscopy of experimental axonal degeneration in photochemically modified Golgi preparations: a procedure for precise mapping of nervous connections. A new version is presented of a procedure for bleaching (deimpregnating) silver chromate Golgi preparations through ultraviolet (UV) light irradiation followed by thiosulphate treatment, leaving only scattered particles of metallic silver. This version gives a better ultrastructure of the tissue than obtained before. Moreover, the results of studying a specific connection in such material are reported. The perforant path, connecting the entorhinal area with the hippocampus and fascia dentata, was transected in the rat and after a 2-day survival the brain was subjected to Golgi impregnation followed by the deimpregnation procedure. Blocks of tissue containing impregnated fascia dentata granule cell dendrites were selected for electron microscopy. Postsynaptic thickenings associated with degenerated boutons could be discerned within the deimpregnated dendrites, proving that the entorhino-dentate fibres end on the dendrites of the granule cells (smooth surfaces as well as spines) and that they end at asymmetric junctions (of Gray's type 1). While this conclusion was as expected from previous experimental studies carried out with different methods it is thought that the same strategy for cell-to-cell mapping of nervous connections may be used also in other parts of the central nervous system."}
{"id": "PMID:1098735", "title": "A Golgi and electron microscopical study of nerve cells in layer I of the cat auditory cortex.", "content": "The nerve cells of the plexiform layer of the cat auditory cortex were studied in Golgi rapid preparations, and by electron microscopy. In the Golgi material 178 cells were found. Thirty were horizontal cells (Cajal's cells) with spiny dendrites and a long axon with horizontal trajectory. Seventy-two small cells had smooth dendrites and a short axon branching profusely around the perikaryon, within the plexiform layer. Twenty-eight were slightly larger cells, morphologically similar to the small ones, but their axons and dendrites were longer, extending to layer II. Twenty-five cells had large bottle-shaped perikarya, spiny dendrites reaching layer II, and a vertically oriented axon branching in layer II, and sometimes reaching layer III. In addition, 23 small modified pyramidal cells, similar to those of layer II, were found within the boundaries of layer I. In the most superficial 50 mum of the cortex horizontal cells were found, but the other categories were very rare. The 3 categories of short axon cells were preferentially localized between 50 and 150 mum from the pial surface. By electron microscopy two classes of cells were found. The first had abundant mitochondria, well defined Nissl bodies and few axosomatic synapses. The second had few mitochondria, undefined Nissl bodies and 6-8 axosomatic synapses per profile. Desmosome-like membrane specializations were found between perikarya of the second class and pyramidal dendrites or their spines. It is suggested that the first class of cells found by electron microscopy may correspond to the horizontal cells while the second class may correspond to the 3 categories of short axon cells.", "contents": "A Golgi and electron microscopical study of nerve cells in layer I of the cat auditory cortex. The nerve cells of the plexiform layer of the cat auditory cortex were studied in Golgi rapid preparations, and by electron microscopy. In the Golgi material 178 cells were found. Thirty were horizontal cells (Cajal's cells) with spiny dendrites and a long axon with horizontal trajectory. Seventy-two small cells had smooth dendrites and a short axon branching profusely around the perikaryon, within the plexiform layer. Twenty-eight were slightly larger cells, morphologically similar to the small ones, but their axons and dendrites were longer, extending to layer II. Twenty-five cells had large bottle-shaped perikarya, spiny dendrites reaching layer II, and a vertically oriented axon branching in layer II, and sometimes reaching layer III. In addition, 23 small modified pyramidal cells, similar to those of layer II, were found within the boundaries of layer I. In the most superficial 50 mum of the cortex horizontal cells were found, but the other categories were very rare. The 3 categories of short axon cells were preferentially localized between 50 and 150 mum from the pial surface. By electron microscopy two classes of cells were found. The first had abundant mitochondria, well defined Nissl bodies and few axosomatic synapses. The second had few mitochondria, undefined Nissl bodies and 6-8 axosomatic synapses per profile. Desmosome-like membrane specializations were found between perikarya of the second class and pyramidal dendrites or their spines. It is suggested that the first class of cells found by electron microscopy may correspond to the horizontal cells while the second class may correspond to the 3 categories of short axon cells."}
{"id": "PMID:1098736", "title": "In vivo localization of brain sulci by arteriography: a stereotactic anatomoradiological study.", "content": "The authors studied the course of the cortical arteries using the Talairach stereotactic system (orthogonal 4.8 m teleradiography, with rigid fixation of the skull in the stereotactic frame) in both anatomical specimens and normal bilateral carotid angiographies of epileptic patients. Anatomoradiological correlations between arterial patterns and underlying brain surface were identified by detailed 3-dimensional analysis of brain-skull preparations, where gyri and injected arteries were dissected progressively with stereotactic X-ray and photographic pictures taken at each step. The vascular landmarks so identified were studied in stereoscopic arteriographies that give a direct visualization of the spatial relationships of the arterial tree moulding the cortical surface thus allowing the recognition of the localization of the principal gyri and sulci. The latter were drawn on stereotactic diagrams. The basic sulcal pattern as shown by the arteries is strikingly symmetrical in both hemispheres of the same individual, in spite of variability in branching of the arterial tree. The disposition of the vascular landmarks in the retroinsular region reflects the left-right asymmetry of the planum temporale that can be recognized in vivo. This can give a clue to the study of anatomofunctional correlations with regard to speech dominance. A limited first series studied in this respect strongly suggests a close relationship between anatomical and functional asymmetry of the human cerebral hemispheres. Generally speaking, the authors feel that 3- dimensional, anatomical interpretation of angiography permits a direct localization of brain structures including the cortex, that can be used instead of indirect localization landmarks in stereotactic explorations, and further, that the wealth of anatomical information that can be drawn from angiography with their method may be helpful in diagnosis or for planning surgery.", "contents": "In vivo localization of brain sulci by arteriography: a stereotactic anatomoradiological study. The authors studied the course of the cortical arteries using the Talairach stereotactic system (orthogonal 4.8 m teleradiography, with rigid fixation of the skull in the stereotactic frame) in both anatomical specimens and normal bilateral carotid angiographies of epileptic patients. Anatomoradiological correlations between arterial patterns and underlying brain surface were identified by detailed 3-dimensional analysis of brain-skull preparations, where gyri and injected arteries were dissected progressively with stereotactic X-ray and photographic pictures taken at each step. The vascular landmarks so identified were studied in stereoscopic arteriographies that give a direct visualization of the spatial relationships of the arterial tree moulding the cortical surface thus allowing the recognition of the localization of the principal gyri and sulci. The latter were drawn on stereotactic diagrams. The basic sulcal pattern as shown by the arteries is strikingly symmetrical in both hemispheres of the same individual, in spite of variability in branching of the arterial tree. The disposition of the vascular landmarks in the retroinsular region reflects the left-right asymmetry of the planum temporale that can be recognized in vivo. This can give a clue to the study of anatomofunctional correlations with regard to speech dominance. A limited first series studied in this respect strongly suggests a close relationship between anatomical and functional asymmetry of the human cerebral hemispheres. Generally speaking, the authors feel that 3- dimensional, anatomical interpretation of angiography permits a direct localization of brain structures including the cortex, that can be used instead of indirect localization landmarks in stereotactic explorations, and further, that the wealth of anatomical information that can be drawn from angiography with their method may be helpful in diagnosis or for planning surgery."}
{"id": "PMID:1098746", "title": "Heat destruction of some bacterial strains attached to broiler skin.", "content": "1. Heat destruction of bacteria attached to the skin did not occur at a logarithmic rate provided the temperature was above 51 degrees C. 2. It was concluded that the bacteria are protected by their location in the skin surface rather than by polymers produced during attachment. 3. An analogous process is considered to take place during the scalding of broilers, since bacteria which survived were difficult to remove from the carcasses during further processing.", "contents": "Heat destruction of some bacterial strains attached to broiler skin. 1. Heat destruction of bacteria attached to the skin did not occur at a logarithmic rate provided the temperature was above 51 degrees C. 2. It was concluded that the bacteria are protected by their location in the skin surface rather than by polymers produced during attachment. 3. An analogous process is considered to take place during the scalding of broilers, since bacteria which survived were difficult to remove from the carcasses during further processing."}
{"id": "PMID:1098747", "title": "Further studies on the attachment of bacteria to skin.", "content": "1. A proportion of the bacterial flora of the skin is present in the surrounding water film and can be removed by adequate rinsing. 2. The remainder of the flora is attached to the skin in such a manner that numbers can be reduced only 10-fold during spray-cooling or spin-chilling. 3. The reduction process was found to be independent of bacterial type or the extent of previous contamination. 4. The significance of the \"water film flora\" is discussed with respect to the attachment of bacteria and the cleaning of carcasses.", "contents": "Further studies on the attachment of bacteria to skin. 1. A proportion of the bacterial flora of the skin is present in the surrounding water film and can be removed by adequate rinsing. 2. The remainder of the flora is attached to the skin in such a manner that numbers can be reduced only 10-fold during spray-cooling or spin-chilling. 3. The reduction process was found to be independent of bacterial type or the extent of previous contamination. 4. The significance of the \"water film flora\" is discussed with respect to the attachment of bacteria and the cleaning of carcasses."}
{"id": "PMID:1098748", "title": "The effectiveness of in-plant chlorination in poultry processing.", "content": "1. Sampling carcasses after plucking or after the post-evisceration spray-wash showed that 10 or 20 ppm available chlorine in the processing-plant water supply caused little reduction in carcass contamination. 2. When 20 ppm chlorine was used in the water supply to parts of the processing-plant other than the mechanical immersion chilling system, counts of faecal and spoilage bacteria from carcasses were reduced approximately 10-fold after passage through the chilling system; bacterial carry-over of chlorine from the final spray-washer. 3. Artificial contamination of carcasses with a readily identifiable strain of Escherichia coli confirmed the occurrence of cross-contamination during plucking and evisceration; in-plant chlorination reduced neither the proportion of carasses contaminated nor the numbers of organisms transferred at these stages. 4. In most cases the chlorine-resistance of poultry spoilage pseudomonads was greater than that of E. coli; hence in-plant chlorination is to be recommended for processing-plant water supplies which carry such spoilage organisms.", "contents": "The effectiveness of in-plant chlorination in poultry processing. 1. Sampling carcasses after plucking or after the post-evisceration spray-wash showed that 10 or 20 ppm available chlorine in the processing-plant water supply caused little reduction in carcass contamination. 2. When 20 ppm chlorine was used in the water supply to parts of the processing-plant other than the mechanical immersion chilling system, counts of faecal and spoilage bacteria from carcasses were reduced approximately 10-fold after passage through the chilling system; bacterial carry-over of chlorine from the final spray-washer. 3. Artificial contamination of carcasses with a readily identifiable strain of Escherichia coli confirmed the occurrence of cross-contamination during plucking and evisceration; in-plant chlorination reduced neither the proportion of carasses contaminated nor the numbers of organisms transferred at these stages. 4. In most cases the chlorine-resistance of poultry spoilage pseudomonads was greater than that of E. coli; hence in-plant chlorination is to be recommended for processing-plant water supplies which carry such spoilage organisms."}
{"id": "PMID:1098751", "title": "Althesin and thiopentone: a clinical comparison.", "content": "Althesin was compared with thiopentone in a double blind study of 200 patients undergoing gynaecological operations. Althesin in a dose of 0.05 ml/kg was found to be an excellent induction agent, virtually devoid of undesirable side effects; however, when used as the principal anaesthetic (supplemented only by 66 per cent nitrous oxide in oxygen), the incidence of disturbing movements was high. Recovery time from Althesin was one-third of that after thiopentone. The patients recovering from Althesin were remarkably clearheaded with a low incidence of \"hangover\".", "contents": "Althesin and thiopentone: a clinical comparison. Althesin was compared with thiopentone in a double blind study of 200 patients undergoing gynaecological operations. Althesin in a dose of 0.05 ml/kg was found to be an excellent induction agent, virtually devoid of undesirable side effects; however, when used as the principal anaesthetic (supplemented only by 66 per cent nitrous oxide in oxygen), the incidence of disturbing movements was high. Recovery time from Althesin was one-third of that after thiopentone. The patients recovering from Althesin were remarkably clearheaded with a low incidence of \"hangover\"."}
{"id": "PMID:1098752", "title": "Percutaneous transtracheal ventilation for anaesthesia and resuscitation: a review and report of complications.", "content": "Percutaneous transtracheal ventilation with intermittent jets of oxygen under high pressure has been used for approximately five years. This paper briefly reviews published reports on the use of this technique for resuscitation and during anaesthesia. Complications occurring in the reports reviewed are tabulated. The complications occurring in a series of 80 patients (52 elective prior to anaesthesia and 28 for acute respiratory distress) at the Pittsburgh Eye and Ear Hospital are documented. This includes three detailed case reports. No deaths and few serious complications have so far been reported, particularly with the elective use of transtracheal ventilation. This technique has established itself as an important adjunct in life support. When used in properly selected cases, its continued use appears justified.", "contents": "Percutaneous transtracheal ventilation for anaesthesia and resuscitation: a review and report of complications. Percutaneous transtracheal ventilation with intermittent jets of oxygen under high pressure has been used for approximately five years. This paper briefly reviews published reports on the use of this technique for resuscitation and during anaesthesia. Complications occurring in the reports reviewed are tabulated. The complications occurring in a series of 80 patients (52 elective prior to anaesthesia and 28 for acute respiratory distress) at the Pittsburgh Eye and Ear Hospital are documented. This includes three detailed case reports. No deaths and few serious complications have so far been reported, particularly with the elective use of transtracheal ventilation. This technique has established itself as an important adjunct in life support. When used in properly selected cases, its continued use appears justified."}
{"id": "PMID:1098755", "title": "Antazoline therapy of recurrent refractory supraventricular arrhythmias--a preliminary report.", "content": "Seven patients with chronic or recurrent supraventricular tachyarrhythmias were selected for a trial of antazoline therapy because sinus rhythm or a controlled ventricular response could not be achieved with quinidine, procainamide, digitalis or propranolol. Sinus rhythm was established by either intravenous administration of antazoline or direct-current countershock, and has been maintained in all for 4 to 16 months by oral administration of antazoline. Side effects were minor. Antazoline is a sufficiently promising antiarrhythmic agent to warrant large-scale controlled studies.", "contents": "Antazoline therapy of recurrent refractory supraventricular arrhythmias--a preliminary report. Seven patients with chronic or recurrent supraventricular tachyarrhythmias were selected for a trial of antazoline therapy because sinus rhythm or a controlled ventricular response could not be achieved with quinidine, procainamide, digitalis or propranolol. Sinus rhythm was established by either intravenous administration of antazoline or direct-current countershock, and has been maintained in all for 4 to 16 months by oral administration of antazoline. Side effects were minor. Antazoline is a sufficiently promising antiarrhythmic agent to warrant large-scale controlled studies."}
{"id": "PMID:1098756", "title": "Aldosterone and renin in essential hypertension.", "content": "A review of some recent laboratory findings indicates definite disturbances in aldosterone metabolism and regulation in patients with mild essential hypertension: (a) a significant mean increase in plasma aldosterone concentration in patients with mild and stable essential hypertension, in contrast to the absence of any difference in patients with labile borderline essential hypertension when in a normotensive phase, compared with control subjects; and (b) a significant mean decrease in metabolic clearance rate of aldosterone, associated with a 12% decrease in hepatic blood flow and an increased binding of aldosterone to a transcortin-like plasma globulin. The secretion rate of 18-hydroxy-11-deoxycorticosterone is above the upper range of normal in 60% of patients with mild, uncomplicated essential hypertension. The incidence of low-renin hypertension, when age and race are taken into account, is much lower than previously assumed. Unless measurements are repeated over a long period, one or two low values of plasma renin cannot be considered a permanent marker indicating a special category of patients with essential hypertension. Tonin, a new enzyme discovered by Boucher, which forms angiotensin II directly from a plasma protein, from the tetradecapeptide substrate and from angiotensin I, is present in most tissues, but in highest concentration in the submaxillary gland. This enzyme is under the control of beta-adrenergic receptors.", "contents": "Aldosterone and renin in essential hypertension. A review of some recent laboratory findings indicates definite disturbances in aldosterone metabolism and regulation in patients with mild essential hypertension: (a) a significant mean increase in plasma aldosterone concentration in patients with mild and stable essential hypertension, in contrast to the absence of any difference in patients with labile borderline essential hypertension when in a normotensive phase, compared with control subjects; and (b) a significant mean decrease in metabolic clearance rate of aldosterone, associated with a 12% decrease in hepatic blood flow and an increased binding of aldosterone to a transcortin-like plasma globulin. The secretion rate of 18-hydroxy-11-deoxycorticosterone is above the upper range of normal in 60% of patients with mild, uncomplicated essential hypertension. The incidence of low-renin hypertension, when age and race are taken into account, is much lower than previously assumed. Unless measurements are repeated over a long period, one or two low values of plasma renin cannot be considered a permanent marker indicating a special category of patients with essential hypertension. Tonin, a new enzyme discovered by Boucher, which forms angiotensin II directly from a plasma protein, from the tetradecapeptide substrate and from angiotensin I, is present in most tissues, but in highest concentration in the submaxillary gland. This enzyme is under the control of beta-adrenergic receptors."}
{"id": "PMID:1098759", "title": "Effect of chronic renal failure, dialysis and transplantation on motor nerve conduction velocity in children.", "content": "Ulnar and peroneal motor nerve conduction volocities (MNCVs) were measured in 47 children in a dialysis-transplantation program. Mean peroneal MNCV was significantly decreased from normal in children with mild renal failure (serum creatinine concentration, 1.5 to 2.9 mg/dl), whereas ulnar MNCV was significantly decreased only when the serum creatinine value was at least 9 mg/dl. Both ulnar and peroneal MNCVs remained unchanged during long-term hemodialysis or peritoneal dialysis; however, after individual dialyses ulnar MNCV increased. After renal transplantation ulnar MNCV returned to normal within a year and peroneal MNCV within 3 years. Before dialysis was required and during long-term dialysis most plasma magnesium values were elevated; ionized calcium activity was decreased in about 50% of determinations. After transplantation and the concentration of divalent cations rapidly returned to normal. These children differed from adults studied in that (a) there was no correlation between severity of renal failure and MNCV, (b) long-term dialysis did not improve MNCV and (c) peroneal velocities did not recover for 3 years after transplantation.", "contents": "Effect of chronic renal failure, dialysis and transplantation on motor nerve conduction velocity in children. Ulnar and peroneal motor nerve conduction volocities (MNCVs) were measured in 47 children in a dialysis-transplantation program. Mean peroneal MNCV was significantly decreased from normal in children with mild renal failure (serum creatinine concentration, 1.5 to 2.9 mg/dl), whereas ulnar MNCV was significantly decreased only when the serum creatinine value was at least 9 mg/dl. Both ulnar and peroneal MNCVs remained unchanged during long-term hemodialysis or peritoneal dialysis; however, after individual dialyses ulnar MNCV increased. After renal transplantation ulnar MNCV returned to normal within a year and peroneal MNCV within 3 years. Before dialysis was required and during long-term dialysis most plasma magnesium values were elevated; ionized calcium activity was decreased in about 50% of determinations. After transplantation and the concentration of divalent cations rapidly returned to normal. These children differed from adults studied in that (a) there was no correlation between severity of renal failure and MNCV, (b) long-term dialysis did not improve MNCV and (c) peroneal velocities did not recover for 3 years after transplantation."}
{"id": "PMID:1098760", "title": "Surgical management of bacterial meningitis.", "content": "A variety of associated lesions may require the neurosurgeon's assistance in the management of bacterial meningitis. As treatment of this infection of the central nervous system proceeds, the surgeon will have to decide about the concurrent or subsequent operative treatment of congenital dysraphic states, paraneural infections, compound fractures or penetrating wounds of thecranium or spine, or infected bypass shunts for cerebrospinal fluid (CSF). In patients with intractable meningitic infections the surgeon may have to insert a ventricular drainage-irrigation system to permit adequate perfusion of the CSF pathways with antibiotic. Hydrocephalus or subdural effusions complicating meningitis may bring the patient to the surgeon long after the infection has been cured. This paper examines these problems and outlines the current principles of management.", "contents": "Surgical management of bacterial meningitis. A variety of associated lesions may require the neurosurgeon's assistance in the management of bacterial meningitis. As treatment of this infection of the central nervous system proceeds, the surgeon will have to decide about the concurrent or subsequent operative treatment of congenital dysraphic states, paraneural infections, compound fractures or penetrating wounds of thecranium or spine, or infected bypass shunts for cerebrospinal fluid (CSF). In patients with intractable meningitic infections the surgeon may have to insert a ventricular drainage-irrigation system to permit adequate perfusion of the CSF pathways with antibiotic. Hydrocephalus or subdural effusions complicating meningitis may bring the patient to the surgeon long after the infection has been cured. This paper examines these problems and outlines the current principles of management."}
{"id": "PMID:1098764", "title": "Combined androgen and antimetabolite therapy of advanced female breast cancer. A report of the cooperative breast cancer group.", "content": "A clinical trial of androgen and antimetabolite therapy of advanced female breast cancer was conducted in 110 patients by the Cooperative Breast Cancer Group. An objective regression rate of 20% was achieved in women receiving oral testolactone, 6% in patients given intravenous fluorouracil alone, and 14% when the androgen and antimetabolite were administered together. This randomized trial according to the CBCG protocol did not produce the high regression rate noted previously in a nonrandomized, nonprotocol evaluation of these drugs.", "contents": "Combined androgen and antimetabolite therapy of advanced female breast cancer. A report of the cooperative breast cancer group. A clinical trial of androgen and antimetabolite therapy of advanced female breast cancer was conducted in 110 patients by the Cooperative Breast Cancer Group. An objective regression rate of 20% was achieved in women receiving oral testolactone, 6% in patients given intravenous fluorouracil alone, and 14% when the androgen and antimetabolite were administered together. This randomized trial according to the CBCG protocol did not produce the high regression rate noted previously in a nonrandomized, nonprotocol evaluation of these drugs."}
{"id": "PMID:1098765", "title": "Proliferation-dependent cytotoxicity of anticancer agents: a review.", "content": "One of the factors of importance in determining the killing of mammalian cells following exposure to a variety of anticancer agents is the proliferative state of the cell population. Generally, proliferating cells are much more sensitive to anticancer agents than are nonproliferating cells. In this review, the cellular aspects of this differential sensitivity are discussed with the hemopoietic stem cell population and tissue culture cells as the focus for the analysis. This phenomenon is not only of concern to the cell biologist but also has implications with regard to scheduling of anticancer agents against human tumors.", "contents": "Proliferation-dependent cytotoxicity of anticancer agents: a review. One of the factors of importance in determining the killing of mammalian cells following exposure to a variety of anticancer agents is the proliferative state of the cell population. Generally, proliferating cells are much more sensitive to anticancer agents than are nonproliferating cells. In this review, the cellular aspects of this differential sensitivity are discussed with the hemopoietic stem cell population and tissue culture cells as the focus for the analysis. This phenomenon is not only of concern to the cell biologist but also has implications with regard to scheduling of anticancer agents against human tumors."}
{"id": "PMID:1098766", "title": "Chromosome studies in acute leukemias developing in patients with multiple myeloma.", "content": "Chromosomal findings are reported in three patients with acute myelomonocytic leukemia and in one with reticulosarcoma leukemia who had been treated for multiple myeloma with melphalan and X-ray. All four patients had striking chromosomal anomalies. An iatrogenic causation of aneuploidy is suggested. This is supported by chromosomal findings in patients with acute leukemia following polycythemia vera and Hodgkin's disease; practically all of the leukemias have been aneuploid. A comparison is made of such \"secondary\" acute leukemias with \"primary\" acute leukemias that are aneuploid in only 40% of the cases. Chromosomal changes are not considered to be the initial event in leukemogenesis.", "contents": "Chromosome studies in acute leukemias developing in patients with multiple myeloma. Chromosomal findings are reported in three patients with acute myelomonocytic leukemia and in one with reticulosarcoma leukemia who had been treated for multiple myeloma with melphalan and X-ray. All four patients had striking chromosomal anomalies. An iatrogenic causation of aneuploidy is suggested. This is supported by chromosomal findings in patients with acute leukemia following polycythemia vera and Hodgkin's disease; practically all of the leukemias have been aneuploid. A comparison is made of such \"secondary\" acute leukemias with \"primary\" acute leukemias that are aneuploid in only 40% of the cases. Chromosomal changes are not considered to be the initial event in leukemogenesis."}
{"id": "PMID:1098779", "title": "A mutant of escherichia coli defective in removing 3' terminal nucleotides from some transfer RNA precursor molecules.", "content": "The conversion of precursor RNA into bacteriophage T4 proline and serine transfer RNAs includes two steps for the enzymatic removal of nucleotides from the 3' ends of RNA chains. Neither of these steps occur following infection of a mutant of Escherichia coli that was previously shown to block the suppressor function of T4 serine transfer RNA. Cell-free extracts of this mutant are furthermore deficient in a wild type enzyme activity that removes nucleotides from the 3' ends of one of the RNA chains described above. The relation of this enzyme to other 3' ribonucleases is not known. We subsequently examined the mutant for its ability to support the biosynthesis of other bacteriophage transfer RNAs.  In one instance that is analogous to the proline-serine precursor RNA, maturation of the precursor RNA was blocked during infection of mutant cells. In another instance, precursor RNA maturation was normal, even though this involved the removal of 3'nucleotides. These observations point to the possible existence of at least two 3' ribonucleases for the biosynthesis of transfer RNAs.", "contents": "A mutant of escherichia coli defective in removing 3' terminal nucleotides from some transfer RNA precursor molecules. The conversion of precursor RNA into bacteriophage T4 proline and serine transfer RNAs includes two steps for the enzymatic removal of nucleotides from the 3' ends of RNA chains. Neither of these steps occur following infection of a mutant of Escherichia coli that was previously shown to block the suppressor function of T4 serine transfer RNA. Cell-free extracts of this mutant are furthermore deficient in a wild type enzyme activity that removes nucleotides from the 3' ends of one of the RNA chains described above. The relation of this enzyme to other 3' ribonucleases is not known. We subsequently examined the mutant for its ability to support the biosynthesis of other bacteriophage transfer RNAs.  In one instance that is analogous to the proline-serine precursor RNA, maturation of the precursor RNA was blocked during infection of mutant cells. In another instance, precursor RNA maturation was normal, even though this involved the removal of 3'nucleotides. These observations point to the possible existence of at least two 3' ribonucleases for the biosynthesis of transfer RNAs."}
{"id": "PMID:1098780", "title": "Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae.", "content": "Mitochondrial DNA replication was examined in mutants for seven different Saccharomyces cerevisiae genes which are essential for nuclear DNA replication. In cdc8 and cdc21, mutants defective in continued replication during the S phase of the cell cycle, mitochondrial DNA replication ceases at the nonpermissive temperature. Replication is temperature sensitive even when these mutants are arrested in the G1 phase of the cell cycle with alpha factor, a condition where mitochondrial DNA replication continues for the equivalent of several generations at the permissive temperature. Therefore the cessation of replication results from a defect in mitochondrial replication per se, rather than from an indirect consequence of cells being blocked in a phase of the cell cycle where mitochondrial DNA is not normally synthesized. Since the temperature-sensitive mutations are recessive, the products of genes cdc8 and cdc21 must be required for both nuclear and mitochondrial DNA replication. In contrast to cdc8 and cdc21, mitochondrial DNA replication continues for a long time at the nonpermissive temperature in five other cell division cycle mutants in which nuclear DNA synthesis ceases within one cell cycle: cdc4, cdc7, and cdc28, which are defective in the initiation of nuclear DNA synthesis, and cdc14 and cdc23, which are defective in nuclear division. The products of these genes, therefore, are apparently not required for the initiation of mitochondrial DNA replication.", "contents": "Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae. Mitochondrial DNA replication was examined in mutants for seven different Saccharomyces cerevisiae genes which are essential for nuclear DNA replication. In cdc8 and cdc21, mutants defective in continued replication during the S phase of the cell cycle, mitochondrial DNA replication ceases at the nonpermissive temperature. Replication is temperature sensitive even when these mutants are arrested in the G1 phase of the cell cycle with alpha factor, a condition where mitochondrial DNA replication continues for the equivalent of several generations at the permissive temperature. Therefore the cessation of replication results from a defect in mitochondrial replication per se, rather than from an indirect consequence of cells being blocked in a phase of the cell cycle where mitochondrial DNA is not normally synthesized. Since the temperature-sensitive mutations are recessive, the products of genes cdc8 and cdc21 must be required for both nuclear and mitochondrial DNA replication. In contrast to cdc8 and cdc21, mitochondrial DNA replication continues for a long time at the nonpermissive temperature in five other cell division cycle mutants in which nuclear DNA synthesis ceases within one cell cycle: cdc4, cdc7, and cdc28, which are defective in the initiation of nuclear DNA synthesis, and cdc14 and cdc23, which are defective in nuclear division. The products of these genes, therefore, are apparently not required for the initiation of mitochondrial DNA replication."}
{"id": "PMID:1098800", "title": "Effect of procaine hydrochloride on DNA repair in Escherichia coli.", "content": "Liquid-holding recovery and rejoining of gamma-radiation-induced DNA single-strand scissions in Escherichia coli could be effectively inhibited by procaine hydrochloride at the concentration of 20 mM. At this concentration, the drug also reversibly altered cellular permeability barrier as evidenced from the uptake of acriflavin by bacterial cells.", "contents": "Effect of procaine hydrochloride on DNA repair in Escherichia coli. Liquid-holding recovery and rejoining of gamma-radiation-induced DNA single-strand scissions in Escherichia coli could be effectively inhibited by procaine hydrochloride at the concentration of 20 mM. At this concentration, the drug also reversibly altered cellular permeability barrier as evidenced from the uptake of acriflavin by bacterial cells."}
{"id": "PMID:1098804", "title": "Myocardial sodium extraction at varied coronary flows in the dog. Estimation of capillary permeability of residue and outflow detection.", "content": "Sudden injections of boluses containing both 131I-albumin and 24NaCl were made into the coronary artery inflow of isolated blood-perfused dog hearts. Indicator dilution curves were recorded using gamma emissions from both the intact heart and the coronary sinus outflow, with plasma flows, Fs, ranging from 0.3 to 1.8 ml/g min-1. Three measures of sodium extraction, E, during transcapillary passage were obtained from each site by comparison of the sodium and albumin curves. The most useful estimates of E were \"instantaneous extractions\" obtained from the later part of the upslope and the peak of the venous dilution curves (coronary sinus) or from the corresponding early phase of washout of the externally monitored curves (intact organ). Extractions were lower at higher flows. Permeability-surface area products, PS, were computed (1) by the formula PS equals -Fsloge(1 - E), (2) by fitting the observed dilution curves with a Krogh capillary-tissue cylinder model, and (3) by the approximating formula PS equals -Fsloge (1 - 1.14E). The two latter approaches provided a correction for back diffusion of tracer from tissue to blood. For sodium, the values of PS averaged 0.88 +/- 0.36 (SD) ml/g min-1, (n equals 52). At high flows, with Fs greater than 1.0 ml/g min-1, the values of PS averaged 1.01 +/- 0.38 ml/g min-1 (N equals 11). Assuming S equals 500 cm2/g and plasma to be 93% water, our findings suggest capillary permeabilities for sodium of about 3.1 times 10(-5) cm/sec.", "contents": "Myocardial sodium extraction at varied coronary flows in the dog. Estimation of capillary permeability of residue and outflow detection. Sudden injections of boluses containing both 131I-albumin and 24NaCl were made into the coronary artery inflow of isolated blood-perfused dog hearts. Indicator dilution curves were recorded using gamma emissions from both the intact heart and the coronary sinus outflow, with plasma flows, Fs, ranging from 0.3 to 1.8 ml/g min-1. Three measures of sodium extraction, E, during transcapillary passage were obtained from each site by comparison of the sodium and albumin curves. The most useful estimates of E were \"instantaneous extractions\" obtained from the later part of the upslope and the peak of the venous dilution curves (coronary sinus) or from the corresponding early phase of washout of the externally monitored curves (intact organ). Extractions were lower at higher flows. Permeability-surface area products, PS, were computed (1) by the formula PS equals -Fsloge(1 - E), (2) by fitting the observed dilution curves with a Krogh capillary-tissue cylinder model, and (3) by the approximating formula PS equals -Fsloge (1 - 1.14E). The two latter approaches provided a correction for back diffusion of tracer from tissue to blood. For sodium, the values of PS averaged 0.88 +/- 0.36 (SD) ml/g min-1, (n equals 52). At high flows, with Fs greater than 1.0 ml/g min-1, the values of PS averaged 1.01 +/- 0.38 ml/g min-1 (N equals 11). Assuming S equals 500 cm2/g and plasma to be 93% water, our findings suggest capillary permeabilities for sodium of about 3.1 times 10(-5) cm/sec."}
{"id": "PMID:1098805", "title": "Influence of surgery on survival in 145 patients with left main coronary artery disease.", "content": "A total of 145 patients with greater than 50% obstruction of the left main coronary artery (LMC) were studied by coronary arteriography. Of the 140 who survived cardiac catheterization, 121 were considered operable by coronary arteriography, and 19 were inoperable. Of the 121 operable patients, 32 were treated medically, and 89 had aortocoronary bypass surgery. Surgical mortality was 11%. The follow-up was from 1 to 72 months with a mean follow-up of 15 months. A survival table was plotted over a 24-month period. At 90 days survival in surgical patients was 85%, medical patients 72%, and inoperable patients 64%. At one year 82% of surgical patients were alive compared with 61% of medically treated operable patients (P less than 0.05). Thereafter, the survival rate remained stable up to 23 months. Survival in inoperable patients was 38% at one year. Improved surgical survival was associated with the presence of preoperative collateral vessels and a left ventricular stroke index greater than 18 g-m/m2. The study demonstrates that survival is improved significantly by surgery in LMC patients. Since mortality occurs early after coronary arteriography, surgery should be offered early to operable patients.", "contents": "Influence of surgery on survival in 145 patients with left main coronary artery disease. A total of 145 patients with greater than 50% obstruction of the left main coronary artery (LMC) were studied by coronary arteriography. Of the 140 who survived cardiac catheterization, 121 were considered operable by coronary arteriography, and 19 were inoperable. Of the 121 operable patients, 32 were treated medically, and 89 had aortocoronary bypass surgery. Surgical mortality was 11%. The follow-up was from 1 to 72 months with a mean follow-up of 15 months. A survival table was plotted over a 24-month period. At 90 days survival in surgical patients was 85%, medical patients 72%, and inoperable patients 64%. At one year 82% of surgical patients were alive compared with 61% of medically treated operable patients (P less than 0.05). Thereafter, the survival rate remained stable up to 23 months. Survival in inoperable patients was 38% at one year. Improved surgical survival was associated with the presence of preoperative collateral vessels and a left ventricular stroke index greater than 18 g-m/m2. The study demonstrates that survival is improved significantly by surgery in LMC patients. Since mortality occurs early after coronary arteriography, surgery should be offered early to operable patients."}
{"id": "PMID:1098806", "title": "Logistic and technical considerations in the treatment of the wounded heart.", "content": "As improvements in the prehospital care of traumatically injured patients have paralleled advancements in cardiovascular surgery, changing concepts in the management of the patient with blunt and penetrating injuries of the heart have occurred. More critically injured patients now arrive at a hospital facility still alive than in former years. Between 1951 and 1974, 350 patients with heart injuries were treated at Harris County Hospital District facilities. Gunshot wounds now predominate, compared with stab wounds in former years. Thirteen patients had rupture of the heart secondary to blunt trauma. Patients arriving with cardiac arrest and cerebral signs of preterminal activity had a 67% survival rate when cardiorraphy was performed in the Trauma Center. In the last four years, 50 patients without cardiac arrest, but frequently with pericardiocentesis as a preoperative adjunct, had an 87% survival rate.", "contents": "Logistic and technical considerations in the treatment of the wounded heart. As improvements in the prehospital care of traumatically injured patients have paralleled advancements in cardiovascular surgery, changing concepts in the management of the patient with blunt and penetrating injuries of the heart have occurred. More critically injured patients now arrive at a hospital facility still alive than in former years. Between 1951 and 1974, 350 patients with heart injuries were treated at Harris County Hospital District facilities. Gunshot wounds now predominate, compared with stab wounds in former years. Thirteen patients had rupture of the heart secondary to blunt trauma. Patients arriving with cardiac arrest and cerebral signs of preterminal activity had a 67% survival rate when cardiorraphy was performed in the Trauma Center. In the last four years, 50 patients without cardiac arrest, but frequently with pericardiocentesis as a preoperative adjunct, had an 87% survival rate."}
{"id": "PMID:1098807", "title": "Postoperative hemodynamics following aortic cross-clamping during aortocoronary bypass surgery.", "content": "Forty-one patients undergoing aortocoronary bypass surgery were divided into two groups: an \"unclamped\" group (17 patients) in whom the aorta was not cross-clamped during operation and a \"clamped\" group (24 patients) in whom the ascending aorta was cross-clamped during performance of distal anastomoses. Myocardial protection was provided during aortic clamping by cooling the anoxic heart with cold saline immersion. Immediate postoperative hemodynamic function was assayed in both groups, as was cardiopulmonary bypass time and cardiopulmonary bypass time/graft. Cardiopulmonary bypass time was 107 minutes in the unclamped group and 87 minutes in the clamped group (P less than 0.05). Cardiopulmonary bypass time/graft was 48 minutes in the unclamped group and 40 minutes in the clamped group (P less than 0.01). Results of hemodynamic studies with values for the unclamped group listed first were as follows: cardiac index (L/min/m2) 2.3 and 2.5 (NS), stroke index (ml/beat/m2) 23 and 25 (NS), left ventricular minute work index (kg-m/min/m2) 3.03 and 2.81 (NS), and stroke work index (g-m/min/m2) 31 and 30 (NS). These data indicate that aortic cross-clamping during performance of distal anastomoses expedites the performance of aortocoronary bypass surgery and does not adversely affect postoperative hemodynamics.", "contents": "Postoperative hemodynamics following aortic cross-clamping during aortocoronary bypass surgery. Forty-one patients undergoing aortocoronary bypass surgery were divided into two groups: an \"unclamped\" group (17 patients) in whom the aorta was not cross-clamped during operation and a \"clamped\" group (24 patients) in whom the ascending aorta was cross-clamped during performance of distal anastomoses. Myocardial protection was provided during aortic clamping by cooling the anoxic heart with cold saline immersion. Immediate postoperative hemodynamic function was assayed in both groups, as was cardiopulmonary bypass time and cardiopulmonary bypass time/graft. Cardiopulmonary bypass time was 107 minutes in the unclamped group and 87 minutes in the clamped group (P less than 0.05). Cardiopulmonary bypass time/graft was 48 minutes in the unclamped group and 40 minutes in the clamped group (P less than 0.01). Results of hemodynamic studies with values for the unclamped group listed first were as follows: cardiac index (L/min/m2) 2.3 and 2.5 (NS), stroke index (ml/beat/m2) 23 and 25 (NS), left ventricular minute work index (kg-m/min/m2) 3.03 and 2.81 (NS), and stroke work index (g-m/min/m2) 31 and 30 (NS). These data indicate that aortic cross-clamping during performance of distal anastomoses expedites the performance of aortocoronary bypass surgery and does not adversely affect postoperative hemodynamics."}
{"id": "PMID:1098808", "title": "The role of sulfinpyrazone in the prevention of arterio-venous shunt thrombosis.", "content": "The effect of sulfinpyrazone on the incidence of thrombosis of arterio-venous shunts was investigated in a double-blind crossover study in 45 patients on chronic hemodialysis over a period of 12 months. The incidence of thrombosis was reduced from 0.64 thrombi per patient month when on placebo to 0.21 thrombi per patient month when on sulfinpryazone (P less than 0.001). The therapeutic effect was more striking in men than in women and became evident within a week of starting the drug. The side effects were minimal, requiring withdrawal from the study of only one patient. This crossover study strengthens the findings in the previous report that sulfinpyrazone is of value in the prevention of thrombosis.", "contents": "The role of sulfinpyrazone in the prevention of arterio-venous shunt thrombosis. The effect of sulfinpyrazone on the incidence of thrombosis of arterio-venous shunts was investigated in a double-blind crossover study in 45 patients on chronic hemodialysis over a period of 12 months. The incidence of thrombosis was reduced from 0.64 thrombi per patient month when on placebo to 0.21 thrombi per patient month when on sulfinpryazone (P less than 0.001). The therapeutic effect was more striking in men than in women and became evident within a week of starting the drug. The side effects were minimal, requiring withdrawal from the study of only one patient. This crossover study strengthens the findings in the previous report that sulfinpyrazone is of value in the prevention of thrombosis."}
{"id": "PMID:1098809", "title": "The status of cardiac transplantation, 1975.", "content": "Since December 1967, 263 human cardiac transplant operations have been performed throughout the world. Eighty-two of these were performed at Stanford University Medical Center, In 1974, 27 such operations were performed, 15 at Stanford Survival rates for the entire Standford series are 48% at one year and 25% at three years; survival rates at one and three years for patients surviving the first three critical months after transplantation are 77% and 42%, respectively. Recipients under the age of 55 years, with New York Heart Association Class IV cardiac disability, are selected for transplant procedures according to criteria dictated by experience over the past seven years. A routine immunsuppressive regimen for organ transplantation, incorporating prednisone, azathioprine, and antithymocyte globulin is employed early postoperatively, and prednisone and azathioprine are used for indefinite maintenance therapy. Acute cardiac graft rejection in nearly all recipients is diagnosed by clinical signs, electrocardiographic changes, and percutaneous transvenous endomyocardial biopsy. Ninety-five percent of acute rejection episodes are reversible with appropriate immunosuppressive treatment, but infectious complications are common and have accounted for 56% of all postoperative deaths. The Stanford experience in cardiac transplantation has demonstrated the potential therapeutic value of this procedure. Maximum survival now extends beyond five years. Satisfactory graft function has been documented in long-term surviving patients, the majority of whom have enjoyed a high degree of social and physical rehabilitation.", "contents": "The status of cardiac transplantation, 1975. Since December 1967, 263 human cardiac transplant operations have been performed throughout the world. Eighty-two of these were performed at Stanford University Medical Center, In 1974, 27 such operations were performed, 15 at Stanford Survival rates for the entire Standford series are 48% at one year and 25% at three years; survival rates at one and three years for patients surviving the first three critical months after transplantation are 77% and 42%, respectively. Recipients under the age of 55 years, with New York Heart Association Class IV cardiac disability, are selected for transplant procedures according to criteria dictated by experience over the past seven years. A routine immunsuppressive regimen for organ transplantation, incorporating prednisone, azathioprine, and antithymocyte globulin is employed early postoperatively, and prednisone and azathioprine are used for indefinite maintenance therapy. Acute cardiac graft rejection in nearly all recipients is diagnosed by clinical signs, electrocardiographic changes, and percutaneous transvenous endomyocardial biopsy. Ninety-five percent of acute rejection episodes are reversible with appropriate immunosuppressive treatment, but infectious complications are common and have accounted for 56% of all postoperative deaths. The Stanford experience in cardiac transplantation has demonstrated the potential therapeutic value of this procedure. Maximum survival now extends beyond five years. Satisfactory graft function has been documented in long-term surviving patients, the majority of whom have enjoyed a high degree of social and physical rehabilitation."}
{"id": "PMID:1098815", "title": "The qualitative nitroblue tetrazolium (NBT) test as a means to differentiate between infection and rejection in renal transplant patients.", "content": "In order to determine whether immunosupression depresses the response of the NBT test to bacterial infections and to note the effect of allograft rejection on this test, a prospective study was carried out on 30 renal transplant recipients. 12 of 30 renal transplant patients developed bacterial infections and in these patients NBT readings were elevated. 12 of the remianing 18 patients who developed rejection episodes showed normal NBT results. All patients were on high doses of steroids and other immunsuppressive agents. We conclude that the NBT test may be of value in diagnosing bacterial infection in the immunsuppressed allograft recipient, and may also be an useful adjunct in the differentiation between allograft infection and bacterial infection.", "contents": "The qualitative nitroblue tetrazolium (NBT) test as a means to differentiate between infection and rejection in renal transplant patients. In order to determine whether immunosupression depresses the response of the NBT test to bacterial infections and to note the effect of allograft rejection on this test, a prospective study was carried out on 30 renal transplant recipients. 12 of 30 renal transplant patients developed bacterial infections and in these patients NBT readings were elevated. 12 of the remianing 18 patients who developed rejection episodes showed normal NBT results. All patients were on high doses of steroids and other immunsuppressive agents. We conclude that the NBT test may be of value in diagnosing bacterial infection in the immunsuppressed allograft recipient, and may also be an useful adjunct in the differentiation between allograft infection and bacterial infection."}
{"id": "PMID:1098817", "title": "Successful renal transplantation in focal glomerular sclerosis: report of two cases.", "content": "The fate of renal allografts has provided much useful information about the pathogenesis of certain glomerular diseases. Idiopathic nephrotic syndrome with focal glomerular sclerosis (FGS) has been reported to recur in transplants. Two patients are reported with idiopathic nephrotic syndrome who progressed to renal failure. Immunopathologic and ultrastructural studies appeared to exclude immune pathogenic mechanisms in both patients, and light microscopic findings were characteristics of FGS. The nephrotic syndrome in one patient was initially steroid-dependent. Both patients underwent bilateral nephrectomy prior to renal transplantation. Graft function and urine protein excretion were normal in both patients 2 and 4 years respectively after transplantation, and transplant biopsies revealed no evidence of recurrent FGS. These results indicate that renal transplantation in patients with idiopathic nephrotic syndrome and FGS is not invariably followed by recurrent glomerular disease and suggest that the responsible pathogenetic mechanism(s) may not always persist.", "contents": "Successful renal transplantation in focal glomerular sclerosis: report of two cases. The fate of renal allografts has provided much useful information about the pathogenesis of certain glomerular diseases. Idiopathic nephrotic syndrome with focal glomerular sclerosis (FGS) has been reported to recur in transplants. Two patients are reported with idiopathic nephrotic syndrome who progressed to renal failure. Immunopathologic and ultrastructural studies appeared to exclude immune pathogenic mechanisms in both patients, and light microscopic findings were characteristics of FGS. The nephrotic syndrome in one patient was initially steroid-dependent. Both patients underwent bilateral nephrectomy prior to renal transplantation. Graft function and urine protein excretion were normal in both patients 2 and 4 years respectively after transplantation, and transplant biopsies revealed no evidence of recurrent FGS. These results indicate that renal transplantation in patients with idiopathic nephrotic syndrome and FGS is not invariably followed by recurrent glomerular disease and suggest that the responsible pathogenetic mechanism(s) may not always persist."}
{"id": "PMID:1098825", "title": "Prostaglandin in calcium and bone metabolism.", "content": "The effect of prostaglandins on bone resorption and formation is reviewed. The prostaglandins probably play a role in pathological changes in bone, e.g., those occurring in immune responses and neoplastic disease. Their physiological role is unknown.", "contents": "Prostaglandin in calcium and bone metabolism. The effect of prostaglandins on bone resorption and formation is reviewed. The prostaglandins probably play a role in pathological changes in bone, e.g., those occurring in immune responses and neoplastic disease. Their physiological role is unknown."}
{"id": "PMID:1098826", "title": "Clinical aspects of thyrocalcitonin.", "content": "Thyrocalcitonin (TCT) is useful as a diagnostic and therapeutic agent in selected human diseases. Elevated plasma levels of TCT occur in patients with medullary carcinoma of the thyroid gland. Asymptomatic relatives of these patients harboring microscopic foci of tumor may have abnormal plasma levels of TCT in the basal state or after provocative stimuli. In both instances the plasma levels of TCT can be used in the diagnosis and management of this thyroid neoplasm. Elevated plasma levels of TCT have also been described recently in subjects with certain extrathyroidal neoplasms or renal failure. Moderate elevations are seen during normal pregnancy and in the neonatal period. Although exogenous TCT has actions on several organs, including bone, the kidneys and the gastrointestinal tract, its physiological role in man, if any, is still unknown. The recently reported measurements of TCT in normal subjects should facilitate the clarification of this issue. TCT has been used as a therapeutic agent in Paget's disease of bone, hypercalcemia of diverse etiologies, osteopenia and several other skeletal disorders. The dramatic improvement of patients with Paget's disease has been a unique therapeutic action of TCT. The therapeutic responses in hypercalcemic subjects given TCT are encouraging but more information is needed about the pharmacology of the hormones in these subjects before conclusions can be formed. At present the therapeutic role of TCT in osteopenia is hypothetical and the results of ongoing and future studies are needed to determine its effects.", "contents": "Clinical aspects of thyrocalcitonin. Thyrocalcitonin (TCT) is useful as a diagnostic and therapeutic agent in selected human diseases. Elevated plasma levels of TCT occur in patients with medullary carcinoma of the thyroid gland. Asymptomatic relatives of these patients harboring microscopic foci of tumor may have abnormal plasma levels of TCT in the basal state or after provocative stimuli. In both instances the plasma levels of TCT can be used in the diagnosis and management of this thyroid neoplasm. Elevated plasma levels of TCT have also been described recently in subjects with certain extrathyroidal neoplasms or renal failure. Moderate elevations are seen during normal pregnancy and in the neonatal period. Although exogenous TCT has actions on several organs, including bone, the kidneys and the gastrointestinal tract, its physiological role in man, if any, is still unknown. The recently reported measurements of TCT in normal subjects should facilitate the clarification of this issue. TCT has been used as a therapeutic agent in Paget's disease of bone, hypercalcemia of diverse etiologies, osteopenia and several other skeletal disorders. The dramatic improvement of patients with Paget's disease has been a unique therapeutic action of TCT. The therapeutic responses in hypercalcemic subjects given TCT are encouraging but more information is needed about the pharmacology of the hormones in these subjects before conclusions can be formed. At present the therapeutic role of TCT in osteopenia is hypothetical and the results of ongoing and future studies are needed to determine its effects."}
{"id": "PMID:1098827", "title": "High density polyethylene prosthetic femoral head replacemnt in the dog.", "content": "A high density polyethylene (RCH 1000) femoral head prosthesis was inserted in 5 large dogs. Periodic clinical and roentgenographic evaluations, and histopathological examination of the replaced joints as well as biomechanical evaluation of the prosthetic joints were done at intervals from 3 weeks to 15 months. HDP is NOT suitable for movable weight-bearing prosthesis articulating with cartilage or bone. Wear of the cartilage and the polyethylene occurred within a few weeks of function and became extensive proportionally to the length of use. The accumulating wear particles of HDP caused a significant foreign body granulomatous reaction which resulted in erosion of bone adjacent to the acetabulum in one hip.", "contents": "High density polyethylene prosthetic femoral head replacemnt in the dog. A high density polyethylene (RCH 1000) femoral head prosthesis was inserted in 5 large dogs. Periodic clinical and roentgenographic evaluations, and histopathological examination of the replaced joints as well as biomechanical evaluation of the prosthetic joints were done at intervals from 3 weeks to 15 months. HDP is NOT suitable for movable weight-bearing prosthesis articulating with cartilage or bone. Wear of the cartilage and the polyethylene occurred within a few weeks of function and became extensive proportionally to the length of use. The accumulating wear particles of HDP caused a significant foreign body granulomatous reaction which resulted in erosion of bone adjacent to the acetabulum in one hip."}
{"id": "PMID:1098822", "title": "Hypochondroplasia.", "content": "Hypochondroplasia is a hereditary form of short-limbed dwarfism which somewhat resembles achondroplasia, but which is clinically, roentgenographically, and genetically distinct. The pertinent findings in 6 new cases are: moderate rhizomelic shortness of stature; normal craniofacial appearance and hand configuration; and later recognition of the presence of the abnormality than in achondroplasia (which can be recognized at birth). A review of the 35 previously described cases in the literature reveals a preponderance of affected females and a high incidence of spontaneous mutation, although when once evident the trait is passed as an autosomal dominant. Two of our patients had seizures, and mental deficiency may be an associated finding although it is by no means usually present. Orthopedists should be aware of this entity, which may be somewhat more common than has been thought, because of the different implications for ultimate height, which is greater in hypochondroplasia than in achondroplasia, the lack of cauda equina compression findings, and the need for genetic counseling.", "contents": "Hypochondroplasia. Hypochondroplasia is a hereditary form of short-limbed dwarfism which somewhat resembles achondroplasia, but which is clinically, roentgenographically, and genetically distinct. The pertinent findings in 6 new cases are: moderate rhizomelic shortness of stature; normal craniofacial appearance and hand configuration; and later recognition of the presence of the abnormality than in achondroplasia (which can be recognized at birth). A review of the 35 previously described cases in the literature reveals a preponderance of affected females and a high incidence of spontaneous mutation, although when once evident the trait is passed as an autosomal dominant. Two of our patients had seizures, and mental deficiency may be an associated finding although it is by no means usually present. Orthopedists should be aware of this entity, which may be somewhat more common than has been thought, because of the different implications for ultimate height, which is greater in hypochondroplasia than in achondroplasia, the lack of cauda equina compression findings, and the need for genetic counseling."}
{"id": "PMID:1098828", "title": "A comparison of patellectomy with prosthetic replacement of the patella.", "content": "Eight adult mongrel dogs were used to compare patellectomy with prosthetic replacement of the patella. All of the dogs who underwent patellectomies displayed functional disturbances when examined four weeks postoperatively. None of the dogs with patellar prostheses displayed discernible functional disturbances. All but one of the dogs with patellectomies developed pathological alterations in the patellar bed of the femur. None of the dogs with patellar prostheses had pathological alterations in the patellar bed of the femur.", "contents": "A comparison of patellectomy with prosthetic replacement of the patella. Eight adult mongrel dogs were used to compare patellectomy with prosthetic replacement of the patella. All of the dogs who underwent patellectomies displayed functional disturbances when examined four weeks postoperatively. None of the dogs with patellar prostheses displayed discernible functional disturbances. All but one of the dogs with patellectomies developed pathological alterations in the patellar bed of the femur. None of the dogs with patellar prostheses had pathological alterations in the patellar bed of the femur."}
{"id": "PMID:1098830", "title": "Radiotherapy in osteosarcoma.", "content": "Radiation therapy in the treatment of osteosarcoma has been considered with respect of the subclinical metastatic disease in the lung and the primary lesion. Emphasis is given to the necessity of coordinating the management plan of the primary lesion with an adjuvant program. Evaluation of the efficacy of a conservative treatment of the primary lesion by radiation therapy and chemotherapy is considered a proper subject for clinical study. A proper biopsy technique and several practical aspects of technique of radiation therapy in management of osteosarcomas of bone are important factors in survival time of individual patients.", "contents": "Radiotherapy in osteosarcoma. Radiation therapy in the treatment of osteosarcoma has been considered with respect of the subclinical metastatic disease in the lung and the primary lesion. Emphasis is given to the necessity of coordinating the management plan of the primary lesion with an adjuvant program. Evaluation of the efficacy of a conservative treatment of the primary lesion by radiation therapy and chemotherapy is considered a proper subject for clinical study. A proper biopsy technique and several practical aspects of technique of radiation therapy in management of osteosarcomas of bone are important factors in survival time of individual patients."}
{"id": "PMID:1098819", "title": "The problem of chondromalacia patellae.", "content": "Daily activity subjects the human patella to forces often several times the individual's body weight. Healthy cartilage can adjust to these forces if they are not too excessive, concentrated, or repetitive. Such abnormal stresses most frequently occur with the disturbance of normal patellar mechanical function. Chondromalacia patellae is the result common to a wide variety of unusual traumata. Treatment must be directed primarily not toward the damaged patellar cartilage but toward a correction of the mechanical abnormality causing it. Until proven otherwise, a young female complaining of knee joint pain, particularly if bilateral, should be considered as suffering from a subluxating patella, with or without chondromalacia patellae.", "contents": "The problem of chondromalacia patellae. Daily activity subjects the human patella to forces often several times the individual's body weight. Healthy cartilage can adjust to these forces if they are not too excessive, concentrated, or repetitive. Such abnormal stresses most frequently occur with the disturbance of normal patellar mechanical function. Chondromalacia patellae is the result common to a wide variety of unusual traumata. Treatment must be directed primarily not toward the damaged patellar cartilage but toward a correction of the mechanical abnormality causing it. Until proven otherwise, a young female complaining of knee joint pain, particularly if bilateral, should be considered as suffering from a subluxating patella, with or without chondromalacia patellae."}
{"id": "PMID:1098823", "title": "Reconstructive surgery in the myelomeningocele hip.", "content": "In 17 myelomeningocele patients with subluxating or dislocated hips, 31 posterolateral iliopsoas were treated by transfers, as described by Sharrard, in addition to complimentary procedures to balance muscle power and center the hip joint. Surgical goals are to stablize the hips within the acetabulum and prevent the occurrence of severe fixed flexion and adduction contractures of the hip and secondary lumbar lordosis. Surgery is performed at about 1 year of age with bilateral adductor transfer to the ischium, followed in 2 weeks by the posterolateral transfer of the iliopsoas muscle, first on one hip and 2 weeks later following with the other hip. Adductor transfer to the ischium reduces the adductor and secondary flexor power of the adductor longus and anterior fibers of the adductor brevis. After transfer it provides some extensor power. The range of abduction is increased. The transferred iliopsoas muscle has a better mechanical advantage in its new function as an abductor and extensor, and the hips were better centered radiographically than the cases with iliopsoas transfer alone. Fewer bony procedures were required to center the hips when the iliopsoas transfer was performed in children before the age of 1 1/2 years.", "contents": "Reconstructive surgery in the myelomeningocele hip. In 17 myelomeningocele patients with subluxating or dislocated hips, 31 posterolateral iliopsoas were treated by transfers, as described by Sharrard, in addition to complimentary procedures to balance muscle power and center the hip joint. Surgical goals are to stablize the hips within the acetabulum and prevent the occurrence of severe fixed flexion and adduction contractures of the hip and secondary lumbar lordosis. Surgery is performed at about 1 year of age with bilateral adductor transfer to the ischium, followed in 2 weeks by the posterolateral transfer of the iliopsoas muscle, first on one hip and 2 weeks later following with the other hip. Adductor transfer to the ischium reduces the adductor and secondary flexor power of the adductor longus and anterior fibers of the adductor brevis. After transfer it provides some extensor power. The range of abduction is increased. The transferred iliopsoas muscle has a better mechanical advantage in its new function as an abductor and extensor, and the hips were better centered radiographically than the cases with iliopsoas transfer alone. Fewer bony procedures were required to center the hips when the iliopsoas transfer was performed in children before the age of 1 1/2 years."}
{"id": "PMID:1098821", "title": "Talectomy in the treatment of the myelomeningocele patient.", "content": "This paper is an analysis of a series of 11 pediatric myelomeningocele patients in whom 20 talectomies were performed for correction of rigid equinovarus of paralytic vertical talus deformities. In most of the patients talectomy achieved a satisfactory correction of the foot deformity with maintenance of the correction for up to 5 years of follow-up in this study. Failure of the procedure related to two factors: (1) severe external rotational contracture of the hips in patients with high spinal lesions; (2) incomplete removal of the talus because of extensive scarring and fibrosis due to prior infection or surgery. Correction of the external rotational deformity of the hip or a derotational supramalleolar osteotomy of the tibia can prevent the first cause of failure. An intra-operative X-ray of the foot can prevent the latter.", "contents": "Talectomy in the treatment of the myelomeningocele patient. This paper is an analysis of a series of 11 pediatric myelomeningocele patients in whom 20 talectomies were performed for correction of rigid equinovarus of paralytic vertical talus deformities. In most of the patients talectomy achieved a satisfactory correction of the foot deformity with maintenance of the correction for up to 5 years of follow-up in this study. Failure of the procedure related to two factors: (1) severe external rotational contracture of the hips in patients with high spinal lesions; (2) incomplete removal of the talus because of extensive scarring and fibrosis due to prior infection or surgery. Correction of the external rotational deformity of the hip or a derotational supramalleolar osteotomy of the tibia can prevent the first cause of failure. An intra-operative X-ray of the foot can prevent the latter."}
{"id": "PMID:1098832", "title": "Folic acid dosage for chronic hemodialysis patients.", "content": "The doses of folic acid, necessary to avoid folic acid deficiency in patients being maintained on hemodialysis, have been estimated to be between 1 and 5 mg daily. To more precisely define an adequate dose of folic acid, 6 anephric patients were studied. The patients were maintained on hemodialysis and received, in a crossover fashion, 1 mg and 5 mg of folic acid for 3 wk. In a second crossover study, 3 anephric patients were first maintained on 0 and then on 1 mg of folic acid after each dialysis for 3 wk periods. Pre- and postdialysis folic acid blood levels were measured and dialyzer clearances of folic acid were determined. The results of this study support the conclusion of the Food and Drug Administration report suggesting that daily doses of 1 mg of folic acid are adequate to sustain therapeutic folate levels. The data further indicate that the administration of 1 mg of folic acid after each dialysis, rather than 1 mg of folic acid daily, can provide adequate folate.", "contents": "Folic acid dosage for chronic hemodialysis patients. The doses of folic acid, necessary to avoid folic acid deficiency in patients being maintained on hemodialysis, have been estimated to be between 1 and 5 mg daily. To more precisely define an adequate dose of folic acid, 6 anephric patients were studied. The patients were maintained on hemodialysis and received, in a crossover fashion, 1 mg and 5 mg of folic acid for 3 wk. In a second crossover study, 3 anephric patients were first maintained on 0 and then on 1 mg of folic acid after each dialysis for 3 wk periods. Pre- and postdialysis folic acid blood levels were measured and dialyzer clearances of folic acid were determined. The results of this study support the conclusion of the Food and Drug Administration report suggesting that daily doses of 1 mg of folic acid are adequate to sustain therapeutic folate levels. The data further indicate that the administration of 1 mg of folic acid after each dialysis, rather than 1 mg of folic acid daily, can provide adequate folate."}
{"id": "PMID:1098835", "title": "Reconstruction of cranial defects.", "content": "We have found that small defects, particularly those within the hairline, are easily reconstructed with methy methacrylate prepared at the operating table. Larger defects, especially in children and active adults, are benefited by autogenous bone grafts and we prefer split rib. When the fabrication facilities are available the preformed silicone implants offer much to the surgeon. They may be sculptured to correct difficult contour problems such as in the supraorbital or forehead region and are especially useful to fill in defects where the bone is present, but depressed. These three methods have many advantages over the metallic implants. There is little complaint of the implant being cold when the patient is exposed to cold weather and a very important fact is that these methods not only provide a radiolucent reconstruction, but also do not affect EEG examination.", "contents": "Reconstruction of cranial defects. We have found that small defects, particularly those within the hairline, are easily reconstructed with methy methacrylate prepared at the operating table. Larger defects, especially in children and active adults, are benefited by autogenous bone grafts and we prefer split rib. When the fabrication facilities are available the preformed silicone implants offer much to the surgeon. They may be sculptured to correct difficult contour problems such as in the supraorbital or forehead region and are especially useful to fill in defects where the bone is present, but depressed. These three methods have many advantages over the metallic implants. There is little complaint of the implant being cold when the patient is exposed to cold weather and a very important fact is that these methods not only provide a radiolucent reconstruction, but also do not affect EEG examination."}
{"id": "PMID:1098836", "title": "Mandibular reconstruction. Current state of the art.", "content": "At the present time several techniques of bone grafting for restoration of mandibular defects are being employed. These methods are well established by clinical trials and animal research. Other methods are in the experimental stage. Regardless of the technique utilized it remains paramount that the surgeon plan the initial procedure with great care as each failure results in diminished blood supply to the graft bed and makes subsequent procedures less likely to succeed. The well known principles of bone grafting involve establishing good nutritional status of the patient, proper preparation of the graft bed by insuring adequate soft tissue and adequate blood supply, elimination of infection from the graft bed, provision of adequate fixation and immobilization of the graft, and careful closure of soft tissues to prevent hematoma formation. These principles remain inviolable if the procedure is to succeed.", "contents": "Mandibular reconstruction. Current state of the art. At the present time several techniques of bone grafting for restoration of mandibular defects are being employed. These methods are well established by clinical trials and animal research. Other methods are in the experimental stage. Regardless of the technique utilized it remains paramount that the surgeon plan the initial procedure with great care as each failure results in diminished blood supply to the graft bed and makes subsequent procedures less likely to succeed. The well known principles of bone grafting involve establishing good nutritional status of the patient, proper preparation of the graft bed by insuring adequate soft tissue and adequate blood supply, elimination of infection from the graft bed, provision of adequate fixation and immobilization of the graft, and careful closure of soft tissues to prevent hematoma formation. These principles remain inviolable if the procedure is to succeed."}
{"id": "PMID:1098862", "title": "A statistical approach to the recognition of inferior wall myocardial infarction by the Q/R ratios.", "content": "To sharpen ECG criteria for diagnosis of inferior wall myocardial infarction (IWMI), the distribution of Q duration and Q/R ratios in leads 3 and aVF in four groups have been analyzed. Two of these groups are teaching samples (200 ECGs of patients with various diagnoses, but in whom no myocardial infarction was found on post-mortem, and 123 ECGs of patients in whom IWMI was found on post-mortem examinations) and two control samples (408 ECGs of healthy men and 60 ECGs of patients with clinically proved IWMI). The chosen combination of conditions Q/R3 greater than 25 percent and Q/RaVF greater than 10 per cent gives minimal number of errors for the teaching samples. The diagnostic rule gave 5-7 percent errors over the control samples. It is shown that use of Q duration greater than 0.04'' gives less satisfactory results in the diagnosis of IWMI.", "contents": "A statistical approach to the recognition of inferior wall myocardial infarction by the Q/R ratios. To sharpen ECG criteria for diagnosis of inferior wall myocardial infarction (IWMI), the distribution of Q duration and Q/R ratios in leads 3 and aVF in four groups have been analyzed. Two of these groups are teaching samples (200 ECGs of patients with various diagnoses, but in whom no myocardial infarction was found on post-mortem, and 123 ECGs of patients in whom IWMI was found on post-mortem examinations) and two control samples (408 ECGs of healthy men and 60 ECGs of patients with clinically proved IWMI). The chosen combination of conditions Q/R3 greater than 25 percent and Q/RaVF greater than 10 per cent gives minimal number of errors for the teaching samples. The diagnostic rule gave 5-7 percent errors over the control samples. It is shown that use of Q duration greater than 0.04'' gives less satisfactory results in the diagnosis of IWMI."}
{"id": "PMID:1098864", "title": "Mode of action of 5-fluorocytosine and mechanisms of resistance.", "content": "Mode of action of 5-fluorocytosine (5-FC) and mechanisms of resistance to the drug are discussed on the basis of experiments performed with Candida albicans ATCC 26790 and with 50 selected clinical isolates of C. albicans belonging to serological type A or B and representing various degrees and models of 5-FC resistance (sensitivity). Incorporation of 5-fluorouridylic acid into RNA appeared as a prerequisite to antifungal activity, although at a given incorporation rate, growth inhibition varied considerably from one strain to the other. The amino acid pool was unbalanced, and there was evidence for disturbance of protein synthesis. These dysfunctions of RNA probably account for growth inhibition and cell death, whereas up to the present, there was no proof of formation of 5-fluorodeoxyuridylic acid nor of subsequent inhibition of thymidylate synthetase. Incorporation of fluorinated pyrimidine into RNA was lower in normally sensitive type B strains than in normally sensitive ones of type A, whereas the frequency of 5-FC-resistant mutants was the same. The two serological types did not differ in the activity of cytosine permease nor in that of cytosine deaminase. Among 29 clinical isolates with 6-FC resistance (or impaired sensitivity) no instance of cytosine permease deficiency was found. Two isolates (belonging to the serological type A) were deficient in cytosine deaminase, whereas the majority was probably deficient in uridine monophosphate pyrophosphorylase or had a surplus of de novo synthesis of pyrimidines. Relative 5-FC resistance was more common than complete resistance.", "contents": "Mode of action of 5-fluorocytosine and mechanisms of resistance. Mode of action of 5-fluorocytosine (5-FC) and mechanisms of resistance to the drug are discussed on the basis of experiments performed with Candida albicans ATCC 26790 and with 50 selected clinical isolates of C. albicans belonging to serological type A or B and representing various degrees and models of 5-FC resistance (sensitivity). Incorporation of 5-fluorouridylic acid into RNA appeared as a prerequisite to antifungal activity, although at a given incorporation rate, growth inhibition varied considerably from one strain to the other. The amino acid pool was unbalanced, and there was evidence for disturbance of protein synthesis. These dysfunctions of RNA probably account for growth inhibition and cell death, whereas up to the present, there was no proof of formation of 5-fluorodeoxyuridylic acid nor of subsequent inhibition of thymidylate synthetase. Incorporation of fluorinated pyrimidine into RNA was lower in normally sensitive type B strains than in normally sensitive ones of type A, whereas the frequency of 5-FC-resistant mutants was the same. The two serological types did not differ in the activity of cytosine permease nor in that of cytosine deaminase. Among 29 clinical isolates with 6-FC resistance (or impaired sensitivity) no instance of cytosine permease deficiency was found. Two isolates (belonging to the serological type A) were deficient in cytosine deaminase, whereas the majority was probably deficient in uridine monophosphate pyrophosphorylase or had a surplus of de novo synthesis of pyrimidines. Relative 5-FC resistance was more common than complete resistance."}
{"id": "PMID:1098865", "title": "Kinetics and mechanisms of action of 'folate synthesis inhibitors', alone or in combination, on Escherichia coli. III. Pyrimethamine, trimethoprim and sulfamethoxazole.", "content": "The inhibitory activity of pyrimethamine (PMA) is 1/290 of the activity of trimethoprim (TMP) against E. coli as evaluated from a plot of C.ko/ko-kapp VS. C. Even at high concentrations the effect of PMA in contrast to TMP seems to be cateriostatic. Combinations of TMP and PMA reveal an additive effect. In PMA-treated cultures, the slope of the logarithmic growth curve decreases after an initial inhibited growth, and a second steady state is established. This second steady state has a different reason than the one observed in TMP-treated cultures; whereas the second phase in TMP-inhibited cultures depends on the number of germs, in the case of PMA it depends on the number of generations. Using prewashed cell cultures, it was shown that there is no influence on the two steady states in PMA-inhibited cultures; for TMP, however, it was shown that the presence of the first phase is due to an antagonist excreted into the culture medium. These observations hint at differences in the mode of action of TMP and PMA in addition to differences in the affinity to the target enzyme dihydrofolate reductase. Combination of PMA with sulfamethoxazole (SMZ) at concentrations where both drugs are acting only bacteriostatically leads to effects considerably greater than would be expected from simple additivity. The kill rate observed is the same as observed for TMP/SMZ combinations despite of the considerable lower activity of PMA and SMZ. The results support the assumption that it might be possible to select drug combinations considering the best pharmacokinetical fit and not necessarily the most effective drugs in the series studied.", "contents": "Kinetics and mechanisms of action of 'folate synthesis inhibitors', alone or in combination, on Escherichia coli. III. Pyrimethamine, trimethoprim and sulfamethoxazole. The inhibitory activity of pyrimethamine (PMA) is 1/290 of the activity of trimethoprim (TMP) against E. coli as evaluated from a plot of C.ko/ko-kapp VS. C. Even at high concentrations the effect of PMA in contrast to TMP seems to be cateriostatic. Combinations of TMP and PMA reveal an additive effect. In PMA-treated cultures, the slope of the logarithmic growth curve decreases after an initial inhibited growth, and a second steady state is established. This second steady state has a different reason than the one observed in TMP-treated cultures; whereas the second phase in TMP-inhibited cultures depends on the number of germs, in the case of PMA it depends on the number of generations. Using prewashed cell cultures, it was shown that there is no influence on the two steady states in PMA-inhibited cultures; for TMP, however, it was shown that the presence of the first phase is due to an antagonist excreted into the culture medium. These observations hint at differences in the mode of action of TMP and PMA in addition to differences in the affinity to the target enzyme dihydrofolate reductase. Combination of PMA with sulfamethoxazole (SMZ) at concentrations where both drugs are acting only bacteriostatically leads to effects considerably greater than would be expected from simple additivity. The kill rate observed is the same as observed for TMP/SMZ combinations despite of the considerable lower activity of PMA and SMZ. The results support the assumption that it might be possible to select drug combinations considering the best pharmacokinetical fit and not necessarily the most effective drugs in the series studied."}
{"id": "PMID:1098866", "title": "Mucolytic and antibiotic effect of a new compound in chronic bronchitis.", "content": "The purpose of the study was the objective evaluation of the mucolytic and antibiotic properties of Fluimucil Antibiotic, a molecular combination of acetylcysteine and thiamphenicol. The preparation was administered to 45 patients in the dose of 3 tablets daily each containing 500 mg of thiamphenical. Statistical analysis of the data obtained showed mucolytic effects on the 4th to 5th day of treatment and marked antibiotic activity. The evaluation is positive particularly if the relatively low dose of antibiotic normally used in chronic bronchitis is considered. The preparation Fluimucil Antibiotic can be recommended for the treatment of chronic bronchitis also in view of its good tolerability.", "contents": "Mucolytic and antibiotic effect of a new compound in chronic bronchitis. The purpose of the study was the objective evaluation of the mucolytic and antibiotic properties of Fluimucil Antibiotic, a molecular combination of acetylcysteine and thiamphenicol. The preparation was administered to 45 patients in the dose of 3 tablets daily each containing 500 mg of thiamphenical. Statistical analysis of the data obtained showed mucolytic effects on the 4th to 5th day of treatment and marked antibiotic activity. The evaluation is positive particularly if the relatively low dose of antibiotic normally used in chronic bronchitis is considered. The preparation Fluimucil Antibiotic can be recommended for the treatment of chronic bronchitis also in view of its good tolerability."}
{"id": "PMID:1098867", "title": "The effect of co-trimoxazole on antitoxin response to tetanus toxoid.", "content": "The effect of a 4-day course of co-trimoxazole (Septrin) on antitoxin response to tetanus vaccine was assessed in a double-blind study involving 22 healthy adults. The tetanus antitoxin levels were measured by in vivo and in vitro methods for up to 8 weeks after the first of two injections of tetanus vaccine, given 4 weeks apart. No sigificant difference was observed in the level of tetanus antitoxin produced in subjects who received co-trimoxazole (Septrin) in recommended therapeutic dosage during the first 4 days of the trial from that in subjects who received placebo tablets. The results indicate that a 4-day course of co-trimoxazole (Septrin) does not affect antibody production.", "contents": "The effect of co-trimoxazole on antitoxin response to tetanus toxoid. The effect of a 4-day course of co-trimoxazole (Septrin) on antitoxin response to tetanus vaccine was assessed in a double-blind study involving 22 healthy adults. The tetanus antitoxin levels were measured by in vivo and in vitro methods for up to 8 weeks after the first of two injections of tetanus vaccine, given 4 weeks apart. No sigificant difference was observed in the level of tetanus antitoxin produced in subjects who received co-trimoxazole (Septrin) in recommended therapeutic dosage during the first 4 days of the trial from that in subjects who received placebo tablets. The results indicate that a 4-day course of co-trimoxazole (Septrin) does not affect antibody production."}
{"id": "PMID:1098868", "title": "Results obtained with combination therapy of VM-26, natulan and prednisolone in generalized Hodgkin's disease.", "content": "By evaluating the results obtained in 50 patients the authors stated that VM-26 given in combination with Natulan and Prednisolone is a drug of value in the therapy of generalized Hodgkin's disease. It cause milder side effects than drugs previously used in combination therapy. Complete or partial remissions were obtained in 84% of patients treated. 75% of the therapy-resistant cases proved to be Hodgkin's sarcoma by the postmortem examination. Remission lasted longer in stage III than in stage IV and the previously untreated patients responded better than the treated one. Definite correlation could not be revealed between histologic type and duration of remission.", "contents": "Results obtained with combination therapy of VM-26, natulan and prednisolone in generalized Hodgkin's disease. By evaluating the results obtained in 50 patients the authors stated that VM-26 given in combination with Natulan and Prednisolone is a drug of value in the therapy of generalized Hodgkin's disease. It cause milder side effects than drugs previously used in combination therapy. Complete or partial remissions were obtained in 84% of patients treated. 75% of the therapy-resistant cases proved to be Hodgkin's sarcoma by the postmortem examination. Remission lasted longer in stage III than in stage IV and the previously untreated patients responded better than the treated one. Definite correlation could not be revealed between histologic type and duration of remission."}
{"id": "PMID:1098869", "title": "Antimalarials: screening of (orally administered) silver solfonamides against Plasmodium berghei.", "content": "Orally administered silver sulfadiazine is effective against Plasmodium berghei. Attempts to synthesize an active analogue with antimalarial activity failed. Although several of the analogues were chemically stable (like silver sulfadiazine) in sodium chloride, none of the analogues were biologically active. This suggests that the antimalarial activity of silver sulfadiazine is stereo-specific.", "contents": "Antimalarials: screening of (orally administered) silver solfonamides against Plasmodium berghei. Orally administered silver sulfadiazine is effective against Plasmodium berghei. Attempts to synthesize an active analogue with antimalarial activity failed. Although several of the analogues were chemically stable (like silver sulfadiazine) in sodium chloride, none of the analogues were biologically active. This suggests that the antimalarial activity of silver sulfadiazine is stereo-specific."}
{"id": "PMID:1098870", "title": "In vitro and in vivo microbiological evaluation of Cephapirin, a new antibiotic.", "content": "The microbiological properties of cephapirin, a new semisynthetis cephalosporin, have been studied. This antibiotic, if compared with ampicillin, shows a greater activity against gram positive bacteria, a lack of sensitivity to staphlococcal beta-lactamase, and a lower sensitivity to those produced by gram-negative bacteria. Useful therapeutic of cephapirin levels can be detected in human serum 6 h after administration of 500 and 1,000 mg parenterally.", "contents": "In vitro and in vivo microbiological evaluation of Cephapirin, a new antibiotic. The microbiological properties of cephapirin, a new semisynthetis cephalosporin, have been studied. This antibiotic, if compared with ampicillin, shows a greater activity against gram positive bacteria, a lack of sensitivity to staphlococcal beta-lactamase, and a lower sensitivity to those produced by gram-negative bacteria. Useful therapeutic of cephapirin levels can be detected in human serum 6 h after administration of 500 and 1,000 mg parenterally."}
{"id": "PMID:1098871", "title": "Doxycycline in the treatment of upper and lower respiratory tract infections. A field trial.", "content": "As part of the Pan-European multi-centre trial of doxycycline in the treatment of respiratory tract infections during the winter of 1973/1974, a field trial was carried out in Austria. 95 physicians provided 540 case reports for evaluation. Very good or good results were obtained in 93.9% of patients and the rate of improvement as indicated by fall in temperature, reduction in cough, sputum and pain, and easing of inflammatory symptoms was rapid; 77.8% of patients showed complete remission of acute symptoms after 3 days of treatment. Only 5.6% of patients suffered from side-effects which were mostly mild. Comparison with a previous field trial in Austria in 1968/1969 showed that doxycycline has retained its efficacy over the years.", "contents": "Doxycycline in the treatment of upper and lower respiratory tract infections. A field trial. As part of the Pan-European multi-centre trial of doxycycline in the treatment of respiratory tract infections during the winter of 1973/1974, a field trial was carried out in Austria. 95 physicians provided 540 case reports for evaluation. Very good or good results were obtained in 93.9% of patients and the rate of improvement as indicated by fall in temperature, reduction in cough, sputum and pain, and easing of inflammatory symptoms was rapid; 77.8% of patients showed complete remission of acute symptoms after 3 days of treatment. Only 5.6% of patients suffered from side-effects which were mostly mild. Comparison with a previous field trial in Austria in 1968/1969 showed that doxycycline has retained its efficacy over the years."}
{"id": "PMID:1098872", "title": "A multi-centre trial of alpha-6-desoxy-5-oxytetracycline (doxycycline).", "content": "23 physicians in Italy took part in a multi-centre clinical trial of doxycycline in the treatment of respiratory tract infections. The case records of 290 patients were assessed with respect to both subjective and objective improvement and clinical cure: most patients were considered improved by the third day and cured by the fifth. The recommended dose of doxycycline was 200 mg on the first day and 100 mg daily thereafter; only 11 of the patients had to continue treatment beyond the tenth day. The antibiotic was extremely well tolerated; gastrointestinal side-effects occurred in 10 patients. Overall, 88.6% of the patients achieved a very good or good result.", "contents": "A multi-centre trial of alpha-6-desoxy-5-oxytetracycline (doxycycline). 23 physicians in Italy took part in a multi-centre clinical trial of doxycycline in the treatment of respiratory tract infections. The case records of 290 patients were assessed with respect to both subjective and objective improvement and clinical cure: most patients were considered improved by the third day and cured by the fifth. The recommended dose of doxycycline was 200 mg on the first day and 100 mg daily thereafter; only 11 of the patients had to continue treatment beyond the tenth day. The antibiotic was extremely well tolerated; gastrointestinal side-effects occurred in 10 patients. Overall, 88.6% of the patients achieved a very good or good result."}
{"id": "PMID:1098873", "title": "Treatment of acute respiratory tract infections with doxycycline in general practice.", "content": "A multi-centre general practitioner trial was carried out in the Netherlands during the winter of 1973/1974. This trial was part of a larger pan-European study. There were 13- cases studied in the Netherlands who were only treated with doxycycline, by 17 physicians. All patients included in the trial had an acute infection of either the upper or lower respiratory tract. tthere were 64 females and 66 males. The results were excellent at 100 patients (76.9%) had a very good result, 21 patients (16.2%) had moderate or fair results, and only 7 patients (5.4%) had poor or negative results. Doxycycline was well tolerated as no side-effects were noted in 122 of the patients (93.8%). In the 8 patients who experienced side-effects they were mild and self-limiting.", "contents": "Treatment of acute respiratory tract infections with doxycycline in general practice. A multi-centre general practitioner trial was carried out in the Netherlands during the winter of 1973/1974. This trial was part of a larger pan-European study. There were 13- cases studied in the Netherlands who were only treated with doxycycline, by 17 physicians. All patients included in the trial had an acute infection of either the upper or lower respiratory tract. tthere were 64 females and 66 males. The results were excellent at 100 patients (76.9%) had a very good result, 21 patients (16.2%) had moderate or fair results, and only 7 patients (5.4%) had poor or negative results. Doxycycline was well tolerated as no side-effects were noted in 122 of the patients (93.8%). In the 8 patients who experienced side-effects they were mild and self-limiting."}
{"id": "PMID:1098874", "title": "Vibramycin in paediatrics. An evaluation of the onset of action and efficacy.", "content": "The effectiveness of and tolerance to doxycycline (Vibramycin, Pfizer) was investigated in a group of 188 children and the radpidity of both subjective and objective improvement was assessed in a second group of 58 children. Doxycycline was found to be very effective. It was possible to analyse the results of treatment in 245 out of the total of 246 patients. Cure was obtained in 168 patients (68.6%) and marked improvement was seen in 59 (24.1%). The overall success rate was thus 92.7%. 18 patients (7.3%) failed to respond; these either had a history of relapsing infections or were infected with resistant organisms. Doxycycline also proved to be rapid in its effect. Rapid subjective improvement was seen in 48 out of 58 patients (84.2%) and rapid objective improvement in 41 patients (70.7%). Tolerance to doxycycline was very good. Treatment had to be interrupted in only one out of 246 patients; there were initial gastrointestinal symptoms in 4 patients. Doxycycline showed itself to be a very suitable antibiotic for use in day-to-day paediatric practice.", "contents": "Vibramycin in paediatrics. An evaluation of the onset of action and efficacy. The effectiveness of and tolerance to doxycycline (Vibramycin, Pfizer) was investigated in a group of 188 children and the radpidity of both subjective and objective improvement was assessed in a second group of 58 children. Doxycycline was found to be very effective. It was possible to analyse the results of treatment in 245 out of the total of 246 patients. Cure was obtained in 168 patients (68.6%) and marked improvement was seen in 59 (24.1%). The overall success rate was thus 92.7%. 18 patients (7.3%) failed to respond; these either had a history of relapsing infections or were infected with resistant organisms. Doxycycline also proved to be rapid in its effect. Rapid subjective improvement was seen in 48 out of 58 patients (84.2%) and rapid objective improvement in 41 patients (70.7%). Tolerance to doxycycline was very good. Treatment had to be interrupted in only one out of 246 patients; there were initial gastrointestinal symptoms in 4 patients. Doxycycline showed itself to be a very suitable antibiotic for use in day-to-day paediatric practice."}
{"id": "PMID:1098875", "title": "A comparative trial of doxycycline and ampicillin in the treatment of acute sinusitis.", "content": "A double-blind comparative clinical trial of doxycycline (200 mg on the first day followed by 100 mg daily for a total of 7 days) and ampicillin (250 mg 4 times daily for 7 days) was performed in 44 patients with acute or chonic sinusitis. Significantly superior results were observed with doxycycline; 90% of the patients responded to doxycycline and 35% to ampicillin. The incidence of side-effects was low and these were of a minor nature, in no case with either drug was discontinuance of treatment necessary. These results imply that in treating chronic bronchitis an antibiotic should be given which is effective not only against the responsible organisms but which is able also to penetrate those tissues (such as the sinuses) that act as a reservoir for these organisms. Doxycycline meets these requirements.", "contents": "A comparative trial of doxycycline and ampicillin in the treatment of acute sinusitis. A double-blind comparative clinical trial of doxycycline (200 mg on the first day followed by 100 mg daily for a total of 7 days) and ampicillin (250 mg 4 times daily for 7 days) was performed in 44 patients with acute or chonic sinusitis. Significantly superior results were observed with doxycycline; 90% of the patients responded to doxycycline and 35% to ampicillin. The incidence of side-effects was low and these were of a minor nature, in no case with either drug was discontinuance of treatment necessary. These results imply that in treating chronic bronchitis an antibiotic should be given which is effective not only against the responsible organisms but which is able also to penetrate those tissues (such as the sinuses) that act as a reservoir for these organisms. Doxycycline meets these requirements."}
{"id": "PMID:1098876", "title": "Doxycycline in respiratory tract infections. Report of a retrospective study in Spain during the winter 1972-1973.", "content": "In the winter of 1972-1973 a multicentre trial of the treatment of upper and lower respiratory tract infections was carried out in Spain using doxycycline. 85 physicians participated in the study and treated 1,653 patients. The infections included acute bronchitis, acute exacerbation of chronic bronchitis, pneumonia, bronchopneumonia, tonsillitis, pharyngitis, trachetis, sinusitis, and otitis media. The majority of the patients were adult out-patients although some children and adolescents were included: 1,011 of the patients were male and 642 female. A number of the signs of respiratory infection such as temperature, cough, pain and inflammatory symptoms were examined. A rapid reduction in intensity and severity was noted in all of these parameters. Tolerance to the antibiotic was excellent. Only minor side-effects were reported and these were mild and mainly limited to the gastro-intestinal tract - in no case was treatment discontinued. The total number of side-effects was 37; they occurred in 31 out of the 1,653 patients. The overall evaluation of results showed a very good or good response in 85% of the patients. It appears from this multicentre study that the efficacy of doxycycline has in no way decreased over the 7 years of its extensive use in Spain. It remains a fast acting and effective antibiotic in upper and lower respiratory tract infections irrespective of age, sex or diagnosis.", "contents": "Doxycycline in respiratory tract infections. Report of a retrospective study in Spain during the winter 1972-1973. In the winter of 1972-1973 a multicentre trial of the treatment of upper and lower respiratory tract infections was carried out in Spain using doxycycline. 85 physicians participated in the study and treated 1,653 patients. The infections included acute bronchitis, acute exacerbation of chronic bronchitis, pneumonia, bronchopneumonia, tonsillitis, pharyngitis, trachetis, sinusitis, and otitis media. The majority of the patients were adult out-patients although some children and adolescents were included: 1,011 of the patients were male and 642 female. A number of the signs of respiratory infection such as temperature, cough, pain and inflammatory symptoms were examined. A rapid reduction in intensity and severity was noted in all of these parameters. Tolerance to the antibiotic was excellent. Only minor side-effects were reported and these were mild and mainly limited to the gastro-intestinal tract - in no case was treatment discontinued. The total number of side-effects was 37; they occurred in 31 out of the 1,653 patients. The overall evaluation of results showed a very good or good response in 85% of the patients. It appears from this multicentre study that the efficacy of doxycycline has in no way decreased over the 7 years of its extensive use in Spain. It remains a fast acting and effective antibiotic in upper and lower respiratory tract infections irrespective of age, sex or diagnosis."}
{"id": "PMID:1098877", "title": "Doxycycline in the treatment of respiratory tract infections. Results of a pan-European multi-centre trial.", "content": "In the winter of 1973-4, general practitioners from seven European countries took part in a multi-centre trial of doxycycline in the treatment of infections of the respiratory tract. The carefully designed protocol was observed by all participants. A total of 1,747 patients were admitted to the trial; their ages ranged from 6 years to over 80. The commonest diagnoses (50%) were acute bronchitis and acute exacerbations of chronic bronchitis. On the recommended dosage of 200 mg doxycycline on the first day, followed by 100 mg daily thereafter (though 200 mg could be continued daily in severe cases), 87% of patients achieved good or very good results. Both subjective (pain) and objective (sputum volume and viscosity, temperature, cough) measures showed rapid improvement, usually by the third to fifth days. Side-effects were minimal and mainly gastrointestinal and caused only 4 patients to discontinue treatment. Overall, doxycycline proved its effectiveness and rapidity of action.", "contents": "Doxycycline in the treatment of respiratory tract infections. Results of a pan-European multi-centre trial. In the winter of 1973-4, general practitioners from seven European countries took part in a multi-centre trial of doxycycline in the treatment of infections of the respiratory tract. The carefully designed protocol was observed by all participants. A total of 1,747 patients were admitted to the trial; their ages ranged from 6 years to over 80. The commonest diagnoses (50%) were acute bronchitis and acute exacerbations of chronic bronchitis. On the recommended dosage of 200 mg doxycycline on the first day, followed by 100 mg daily thereafter (though 200 mg could be continued daily in severe cases), 87% of patients achieved good or very good results. Both subjective (pain) and objective (sputum volume and viscosity, temperature, cough) measures showed rapid improvement, usually by the third to fifth days. Side-effects were minimal and mainly gastrointestinal and caused only 4 patients to discontinue treatment. Overall, doxycycline proved its effectiveness and rapidity of action."}
{"id": "PMID:1098878", "title": "Social interaction, social competence, and friendship in children.", "content": "The relationship between social skills, social interaction, and popularity was examined. The subjects were 198 children in third and fourth grades in middle- and low-income schools. The relationships between number of friends, socioeconomic status, and grade level were studied in a 2 times 2 times 2 factorial design with 2 sets of dependent measures: (1) social skills were assessed by an experimenter testing each child individually on a set of tasks which included measures of the ability to label emotions in facial expressions, knowledge of how to make friends, giving help, and role-taking ability; and (2) social interaction in the classroom was assessed using a naturalistic observational system. Popular and unpopular children differed in their knowledge of how to make friends and on the referential-communication task. In the classroom, popular children distributed and received more positive reinforcement than unpopular children and spent less time daydreaming. Both grade and social class factors were significant. However, different patterns of results contributed to the main effect of friends and the grade-level main effect. The importance of assessing social skills which are first validated by reference to a criterion such as sociometric position was noted.", "contents": "Social interaction, social competence, and friendship in children. The relationship between social skills, social interaction, and popularity was examined. The subjects were 198 children in third and fourth grades in middle- and low-income schools. The relationships between number of friends, socioeconomic status, and grade level were studied in a 2 times 2 times 2 factorial design with 2 sets of dependent measures: (1) social skills were assessed by an experimenter testing each child individually on a set of tasks which included measures of the ability to label emotions in facial expressions, knowledge of how to make friends, giving help, and role-taking ability; and (2) social interaction in the classroom was assessed using a naturalistic observational system. Popular and unpopular children differed in their knowledge of how to make friends and on the referential-communication task. In the classroom, popular children distributed and received more positive reinforcement than unpopular children and spent less time daydreaming. Both grade and social class factors were significant. However, different patterns of results contributed to the main effect of friends and the grade-level main effect. The importance of assessing social skills which are first validated by reference to a criterion such as sociometric position was noted."}
{"id": "PMID:1098880", "title": "[Clinical picture and pathomorphology of traumatic high-pressure oil injections, the so called \"grease gun injury\"].", "content": "On the basis of 3 personal observations the clinical picture, pathomorphology and therapy of the so-called \"grease gun injury\", are described. Clinically there is a difference between the acute stage, intermediary stage and chronic stage. In accordance with the course of disease are the pathomophological alterations, which are characterized by the development of an epithelioidcell-like granulation tissue. The injected oil is surrounded by histiocytic cell infiltrations, which, due to the chemico-toxic influence of this foreign substance are transformed into epithloidcells. Analogous to the pathogenesis of the tuberculoid granuloma this cellular metamorphosis is based on the action of long-chain fatty acids. It is important that the injured person is given immediate surgical treatment and operation. The aim of this being the entire removal of the oil or grease in order to avoid chronically granulating inflammation of the soft parts as well as the formation of scar tissue.", "contents": "[Clinical picture and pathomorphology of traumatic high-pressure oil injections, the so called \"grease gun injury\"]. On the basis of 3 personal observations the clinical picture, pathomorphology and therapy of the so-called \"grease gun injury\", are described. Clinically there is a difference between the acute stage, intermediary stage and chronic stage. In accordance with the course of disease are the pathomophological alterations, which are characterized by the development of an epithelioidcell-like granulation tissue. The injected oil is surrounded by histiocytic cell infiltrations, which, due to the chemico-toxic influence of this foreign substance are transformed into epithloidcells. Analogous to the pathogenesis of the tuberculoid granuloma this cellular metamorphosis is based on the action of long-chain fatty acids. It is important that the injured person is given immediate surgical treatment and operation. The aim of this being the entire removal of the oil or grease in order to avoid chronically granulating inflammation of the soft parts as well as the formation of scar tissue."}
{"id": "PMID:1098886", "title": "[Results of the synchronization therapy with vincristine and cyclophosphamide in lymphogranulomatosis, reticulum cell sarcoma and lymphosarcoma. A phase II study (author's transl)].", "content": "The effectiveness of synchronization therapy was tested on 88 patients who had already undergone some form of treatment for lymphogranulomatosis (stage III B and IV), generalized reticulum cell sarcoma or lymphosarcoma. This therapeutical concept is based on a partial synchronic increase in tumor cells induced by noncytocidal doses of vincristine, followed by cytostatic or cytocidal treatment with cyclophosphamide during DNA synthesis of the partially synchronized tumor cells. The therapeutic plan is similar to a single-agent therapy. In lymphogranulomatosis, complete remission could be achieved in 14 out of 19 cases (stage III B) and in 9 out of 24 cases (stage IV). The mean remission period during an orally administered cytostatic maintenance therapy was 15 1/2 months. The highest rate of remission was found in the mixed cell type of granulomas (23 out of 24 patients). In the non-Hodgkin's lymphomas, complete remission was achieved in 13 out of 30 cases (lymphosarcoma) and in 19 out of 15 cases (reticulum cell sarcoma). The mean remission period for orally administered cytostatic maintenance therapy was 16 months for lymphosarcoma and 11 1/2 months for reticulum cell sarcoma. These therapeutic results are comparable to those achieved by very effective schemes of combination chemotherapy but the toxic side-effects of synchronization therapy are considerably lower.", "contents": "[Results of the synchronization therapy with vincristine and cyclophosphamide in lymphogranulomatosis, reticulum cell sarcoma and lymphosarcoma. A phase II study (author's transl)]. The effectiveness of synchronization therapy was tested on 88 patients who had already undergone some form of treatment for lymphogranulomatosis (stage III B and IV), generalized reticulum cell sarcoma or lymphosarcoma. This therapeutical concept is based on a partial synchronic increase in tumor cells induced by noncytocidal doses of vincristine, followed by cytostatic or cytocidal treatment with cyclophosphamide during DNA synthesis of the partially synchronized tumor cells. The therapeutic plan is similar to a single-agent therapy. In lymphogranulomatosis, complete remission could be achieved in 14 out of 19 cases (stage III B) and in 9 out of 24 cases (stage IV). The mean remission period during an orally administered cytostatic maintenance therapy was 15 1/2 months. The highest rate of remission was found in the mixed cell type of granulomas (23 out of 24 patients). In the non-Hodgkin's lymphomas, complete remission was achieved in 13 out of 30 cases (lymphosarcoma) and in 19 out of 15 cases (reticulum cell sarcoma). The mean remission period for orally administered cytostatic maintenance therapy was 16 months for lymphosarcoma and 11 1/2 months for reticulum cell sarcoma. These therapeutic results are comparable to those achieved by very effective schemes of combination chemotherapy but the toxic side-effects of synchronization therapy are considerably lower."}
{"id": "PMID:1098887", "title": "[Effect of slow-release aminophylline on pulmonary function in obstructive respiratory disease(author's transl)].", "content": "The effects on pulmonary function of a slow-release preparation of an oral broncholytic drug (containing 350 mg aminophylline, released over eight hours) was tested on 26 patients in a double-blind trial. There was a marked reduction of airway resistance and stimulation of breathing with decreased dynamic work of breathing. Distinctly improved alveolar function was demonstrated especially in a fall of arterial CO2 tension, but in some cases there was probably an increase in distribution abnormality. Central haemodynamic changes with a decreased in pulmonary artery pressure and changes in the other values lasted for more than ten hours on the first day of treatment, and were demonstrable on the fourth day even before the drug was taken that day. The criteria of an effective broncholytic slow-release drug with sustained effect were thus fulfilled.", "contents": "[Effect of slow-release aminophylline on pulmonary function in obstructive respiratory disease(author's transl)]. The effects on pulmonary function of a slow-release preparation of an oral broncholytic drug (containing 350 mg aminophylline, released over eight hours) was tested on 26 patients in a double-blind trial. There was a marked reduction of airway resistance and stimulation of breathing with decreased dynamic work of breathing. Distinctly improved alveolar function was demonstrated especially in a fall of arterial CO2 tension, but in some cases there was probably an increase in distribution abnormality. Central haemodynamic changes with a decreased in pulmonary artery pressure and changes in the other values lasted for more than ten hours on the first day of treatment, and were demonstrable on the fourth day even before the drug was taken that day. The criteria of an effective broncholytic slow-release drug with sustained effect were thus fulfilled."}
{"id": "PMID:1098888", "title": "[Fludrocortisone in the treatment of orthostatic hypotension: ophthalmodynamography during standing(author's transl)].", "content": "The effect of fludrocortisone (Astonin -H) in the management of orthostatic hypotension was tested in a double-blind trial of 30 patients who were receiving 0.3 mg fludrocortisone for seven days, a control group of 30 subjects receiving placebos. Systolic and diastolic brachial and ophthalmic arterial pressures, pulse amplitude and pulse rate were measured at rest, immediately after standing up and 10 minutes later. Comparing the treatment and placebo groups, the former had a higher systolic ophthalmic blood pressure on standing, a smaller fall of pulse volume and less of a rise in heart rate on standing up, and a raised systolic brachial artery pressure even in recumbency. At the same time there was an improvement in symptoms.", "contents": "[Fludrocortisone in the treatment of orthostatic hypotension: ophthalmodynamography during standing(author's transl)]. The effect of fludrocortisone (Astonin -H) in the management of orthostatic hypotension was tested in a double-blind trial of 30 patients who were receiving 0.3 mg fludrocortisone for seven days, a control group of 30 subjects receiving placebos. Systolic and diastolic brachial and ophthalmic arterial pressures, pulse amplitude and pulse rate were measured at rest, immediately after standing up and 10 minutes later. Comparing the treatment and placebo groups, the former had a higher systolic ophthalmic blood pressure on standing, a smaller fall of pulse volume and less of a rise in heart rate on standing up, and a raised systolic brachial artery pressure even in recumbency. At the same time there was an improvement in symptoms."}
{"id": "PMID:1098904", "title": "[Work-hygienic studies connected with the manipulation of silver-tin-mercury alloys at the dentists working place].", "content": "By means of a spectro-photometric measuring method, tests are made regarding the development of Hg vapors at the dental working place when processing amalgam. Depending on the step in amalgam processing and the location, more or less high Hg vapors concentrations, were measured. They are highest with high-speed, dry preparation of amalgam fillings, they decrease considerably with lower speed and more intensive colling (spray!).", "contents": "[Work-hygienic studies connected with the manipulation of silver-tin-mercury alloys at the dentists working place]. By means of a spectro-photometric measuring method, tests are made regarding the development of Hg vapors at the dental working place when processing amalgam. Depending on the step in amalgam processing and the location, more or less high Hg vapors concentrations, were measured. They are highest with high-speed, dry preparation of amalgam fillings, they decrease considerably with lower speed and more intensive colling (spray!)."}
{"id": "PMID:1098905", "title": "Limbic system involvement in the increase in plasma prolactin following cortical spreading depression in gonadotropin-treated female rats.", "content": "Cortical spreading depression (SD) was induced by applying 25% KCl to the frontal cerebral cortex in female rats under continuous ether anesthesia. Three weeks previously the animals had been subjected to sham operation, bilateral surgical \"deefferentation\" of the amygdala or transection of the dorsal columns of the fornix. During the week prior to experiment the rats were made \"pseudopregnant\" by treatment with PMS and hCG. Plasma prolactin was measured by radioimmunoassay in blood samples obtained from the peripheral circulation at 20 min intervals. After two control samples had been taken, KCl was applied to the cortex and sampling continued for another 100 min; In the sham-operated group prolactin levels increased with time following the application of KCl. Fornix-cut animals showed a similar, although briefer, increase with values significantly lower than those found in sham-operated animals at 80 min. The increase in plasma prolactin observed in sham-operated and fornix groups was completely abolished in amygdala-cut animals. These results indicate that limbic structures play a significant role in the mechanisms by which cortical SD elevates plasma prolactin levels under the present experimental conditions.", "contents": "Limbic system involvement in the increase in plasma prolactin following cortical spreading depression in gonadotropin-treated female rats. Cortical spreading depression (SD) was induced by applying 25% KCl to the frontal cerebral cortex in female rats under continuous ether anesthesia. Three weeks previously the animals had been subjected to sham operation, bilateral surgical \"deefferentation\" of the amygdala or transection of the dorsal columns of the fornix. During the week prior to experiment the rats were made \"pseudopregnant\" by treatment with PMS and hCG. Plasma prolactin was measured by radioimmunoassay in blood samples obtained from the peripheral circulation at 20 min intervals. After two control samples had been taken, KCl was applied to the cortex and sampling continued for another 100 min; In the sham-operated group prolactin levels increased with time following the application of KCl. Fornix-cut animals showed a similar, although briefer, increase with values significantly lower than those found in sham-operated animals at 80 min. The increase in plasma prolactin observed in sham-operated and fornix groups was completely abolished in amygdala-cut animals. These results indicate that limbic structures play a significant role in the mechanisms by which cortical SD elevates plasma prolactin levels under the present experimental conditions."}
{"id": "PMID:1098906", "title": "The effects of Methallibure (ICI 33,828) on the LH release in castrate male rats challenged with LHRH.", "content": "Daily administration of 10 mg of Methallibure (ICI 33,828) for 6 days to male castrate rats resulted in significant depression of serum radioimmunoassayable luteinizing hormone (LH). Subsequent challenge with 100 ng of synthetic luteinizing hormone-releasing hormone (LHRH) showed these rats to release sufficient pituitary LH to achieve plasma levels equal to those of LHRH treated castrate controls. Although an effect at the level of the pituitary remains to be conclusively ruled out, these results suggest that the predominant in vivo effect of Methallibure in the castrate male rat is to suppress pituitary LH release due to diminished secretion of LHRH by the hypothalamus.", "contents": "The effects of Methallibure (ICI 33,828) on the LH release in castrate male rats challenged with LHRH. Daily administration of 10 mg of Methallibure (ICI 33,828) for 6 days to male castrate rats resulted in significant depression of serum radioimmunoassayable luteinizing hormone (LH). Subsequent challenge with 100 ng of synthetic luteinizing hormone-releasing hormone (LHRH) showed these rats to release sufficient pituitary LH to achieve plasma levels equal to those of LHRH treated castrate controls. Although an effect at the level of the pituitary remains to be conclusively ruled out, these results suggest that the predominant in vivo effect of Methallibure in the castrate male rat is to suppress pituitary LH release due to diminished secretion of LHRH by the hypothalamus."}
{"id": "PMID:1098908", "title": "Recent research into the nutrition of the horse.", "content": "The majority of recent experiments in equine nutrition relates to the metabolism of protein, non-protein nitrogen, carbohydrate, calcium and phosphorus. An understanding of the significance of the nitrogen cycle is emerging in which there is a two-way movement of compounds across the wall of the G.I. tract. Some quantitative estimates of the role of microflora in this process have been made for comparison with similar processes in ruminants. However, the pathway by which the microflora contributes to the nitrogen economy and the extent to which this occurs is not established in the horse. It is well established that both glucose and volatile fatty acids are common substrates in tissue metabolism; but in contrast to evidence in the ruminant the ratio of starch to fibre in the diet does not affect the efficiency fo fibre digestibility. This, and similar observations, are explicable in terms of the anatomy of the G.I. tract of the horse. There is a scarcity of information on the secretion of digestive enzymes by the horse and on the relationship between intestinal function and certain digestive upsets. Furthermore, there is an urgent need for accurate clinical evidence for the significance of calcium and phosphorus nutrition to the development of leg abnormalities in horses. The irrelevance, for diagnosing purposes, of estimates of serum Ca and P is concluded, especially where no other relevant information is available. Haematological procedures, of diagnostic value in this respect, are available for use at least in controlled clinical conditions.", "contents": "Recent research into the nutrition of the horse. The majority of recent experiments in equine nutrition relates to the metabolism of protein, non-protein nitrogen, carbohydrate, calcium and phosphorus. An understanding of the significance of the nitrogen cycle is emerging in which there is a two-way movement of compounds across the wall of the G.I. tract. Some quantitative estimates of the role of microflora in this process have been made for comparison with similar processes in ruminants. However, the pathway by which the microflora contributes to the nitrogen economy and the extent to which this occurs is not established in the horse. It is well established that both glucose and volatile fatty acids are common substrates in tissue metabolism; but in contrast to evidence in the ruminant the ratio of starch to fibre in the diet does not affect the efficiency fo fibre digestibility. This, and similar observations, are explicable in terms of the anatomy of the G.I. tract of the horse. There is a scarcity of information on the secretion of digestive enzymes by the horse and on the relationship between intestinal function and certain digestive upsets. Furthermore, there is an urgent need for accurate clinical evidence for the significance of calcium and phosphorus nutrition to the development of leg abnormalities in horses. The irrelevance, for diagnosing purposes, of estimates of serum Ca and P is concluded, especially where no other relevant information is available. Haematological procedures, of diagnostic value in this respect, are available for use at least in controlled clinical conditions."}
{"id": "PMID:1098912", "title": "Studies on the oxygen supply of heterotopic auxiliary liver grafts in rats.", "content": "Heterotopic auxiliary liver transplantations were performed in rats using the method of Hess et. al. [6]. Oxygen pressure of liver tissue was measured with a Pt-surface electrode of Kessler and L\u00fcbbers [10]. After ligation of the hepatic artery, liver tissue PO2 decreased and remained low during further preparation. Only two of the seven lobes of the rat liver (= 30% of the parenchyma) were used as a graft. After revascularization and application of a gelatine plasma substitute, tissue PO2 of the graft increased to values of normal rat liver, although the graft was supplied with portal blood only. We suggest that one reason for this is the increase of portal perfusion rate, for the whole amount of portal blood perfuses only 30% of liver parenchyma. In the first month after transplantation PO2 of the graft parenchyma was low, but increased continually in the following months, reaching PO2 values of normal rat livers after 1 year.", "contents": "Studies on the oxygen supply of heterotopic auxiliary liver grafts in rats. Heterotopic auxiliary liver transplantations were performed in rats using the method of Hess et. al. [6]. Oxygen pressure of liver tissue was measured with a Pt-surface electrode of Kessler and L\u00fcbbers [10]. After ligation of the hepatic artery, liver tissue PO2 decreased and remained low during further preparation. Only two of the seven lobes of the rat liver (= 30% of the parenchyma) were used as a graft. After revascularization and application of a gelatine plasma substitute, tissue PO2 of the graft increased to values of normal rat liver, although the graft was supplied with portal blood only. We suggest that one reason for this is the increase of portal perfusion rate, for the whole amount of portal blood perfuses only 30% of liver parenchyma. In the first month after transplantation PO2 of the graft parenchyma was low, but increased continually in the following months, reaching PO2 values of normal rat livers after 1 year."}
{"id": "PMID:1098913", "title": "Early recovery of albumin synthesis after liver transplantation.", "content": "The delay necessary for an orthotopic transplanted liver to recover in normal activity has never been defined. This is of great importance whenever liver transplantation is considered in patients with severe liver insufficiency. This delay was studied in dogs using albumin synthesis and BSP clearance as tests of liver function. Albumin synthesis was normal as early as 2 h following graft revascularization while BSP clearance was not yet normal by this time. It is suggested that transplanted livers will be rapidly able to corect metabolic disorders and above all coagulation problems in patients with terminal hepatic failure.", "contents": "Early recovery of albumin synthesis after liver transplantation. The delay necessary for an orthotopic transplanted liver to recover in normal activity has never been defined. This is of great importance whenever liver transplantation is considered in patients with severe liver insufficiency. This delay was studied in dogs using albumin synthesis and BSP clearance as tests of liver function. Albumin synthesis was normal as early as 2 h following graft revascularization while BSP clearance was not yet normal by this time. It is suggested that transplanted livers will be rapidly able to corect metabolic disorders and above all coagulation problems in patients with terminal hepatic failure."}
{"id": "PMID:1098914", "title": "Glucagon and insulin release from the allografted canine pancreas.", "content": "Six previously pancreatectomized dogs were transplanted with duct-ligated, pancreatic allografts. Glucagon and insulin levels in the venous outflow from the graft and in the systemic circulation were determined during the first 60 minutes after transplantation. Two of the dogs were subjected to L-arginine stimulation 5 days after transplantation and the glucagon levels in the venous outflow from the graft were determined. The glucagon and insulin concentrations increased rapidly after transplantation, describing a biphasic curve. In response to L-arginine stimulation, an increased glucagon release was observed.", "contents": "Glucagon and insulin release from the allografted canine pancreas. Six previously pancreatectomized dogs were transplanted with duct-ligated, pancreatic allografts. Glucagon and insulin levels in the venous outflow from the graft and in the systemic circulation were determined during the first 60 minutes after transplantation. Two of the dogs were subjected to L-arginine stimulation 5 days after transplantation and the glucagon levels in the venous outflow from the graft were determined. The glucagon and insulin concentrations increased rapidly after transplantation, describing a biphasic curve. In response to L-arginine stimulation, an increased glucagon release was observed."}
{"id": "PMID:1098918", "title": "Measurement of erythropoiesis by radio-iron incorporation: influence of iron- and cell kinetic changes.", "content": "59Fe blood appearance curves have been obtained in mice by injection of the isotope at various times after 850 rad whole body X-irradiation and bone marrow grafting in order to produce different levels of erythropoiesis. The results demonstrate that measurements at 24, 48 and 72 hours are not comparable and lead to different interpretations. Standardization of the time at which 59Fe uptake measurements are made is therefore recommended.", "contents": "Measurement of erythropoiesis by radio-iron incorporation: influence of iron- and cell kinetic changes. 59Fe blood appearance curves have been obtained in mice by injection of the isotope at various times after 850 rad whole body X-irradiation and bone marrow grafting in order to produce different levels of erythropoiesis. The results demonstrate that measurements at 24, 48 and 72 hours are not comparable and lead to different interpretations. Standardization of the time at which 59Fe uptake measurements are made is therefore recommended."}
{"id": "PMID:1098919", "title": "The effects of antilymphocyte sera on bone marrow allograft rejection.", "content": "Prior sensitization can be a problem in successful graft take in bone marrow therapy. Spleen weight and femoral marrow cellularity have been used as indices of successful graft take in lethally irradiated mice receiving bone marrow allografts. Recovery if poor in mice sensitized, at least 6 days before irradiation and bone marrow therapy, to an intraperitoneal injection of allogenic bone marrow cells. Rabbit anti-mouse lymphocyte serum, normal rabbit serum and pig anti-mouse lymphocyte serum but not normal pig serum partially ablate tis immunity when administered 8 and 10 days after sensitization. Normal rabbit serum is, however, ineffective when administratered 26 and 28 days after sensitization whereas rabbit anti-mouse lymphocyte serum completely ablates this immunity. These findings demonstrate the marked radioresistance of the secondary response to bone marrow allografts and further show the effectiveness of antilymphocyte sera in ablating this immunity.", "contents": "The effects of antilymphocyte sera on bone marrow allograft rejection. Prior sensitization can be a problem in successful graft take in bone marrow therapy. Spleen weight and femoral marrow cellularity have been used as indices of successful graft take in lethally irradiated mice receiving bone marrow allografts. Recovery if poor in mice sensitized, at least 6 days before irradiation and bone marrow therapy, to an intraperitoneal injection of allogenic bone marrow cells. Rabbit anti-mouse lymphocyte serum, normal rabbit serum and pig anti-mouse lymphocyte serum but not normal pig serum partially ablate tis immunity when administered 8 and 10 days after sensitization. Normal rabbit serum is, however, ineffective when administratered 26 and 28 days after sensitization whereas rabbit anti-mouse lymphocyte serum completely ablates this immunity. These findings demonstrate the marked radioresistance of the secondary response to bone marrow allografts and further show the effectiveness of antilymphocyte sera in ablating this immunity."}
{"id": "PMID:1098920", "title": "Quantitative measurements on the haemopoietic systems of three strains of mice.", "content": "Quantitative measurements on the haemopoiedic systems of SAS/4, 3H and RFM mice have been used to calculate the distribution and population sizes of haemopoietic cells in the bone marrow, spleen and peripheral blood. Considerable strain differences have been found in the sizes of haemopioetic stem cell populations and in the distribution of these cells within the haemopioetic system, although the incidence of CFU-S was shown to be uniform throughout skeletal sites of haemopioesis. The seeding efficiency of colony forming cells in the spleen, measured by the 'f\" factor 24 hours after irradiation and transplantation, also differs between strains and between the bone marrow and spleen of the strains investigated.", "contents": "Quantitative measurements on the haemopoietic systems of three strains of mice. Quantitative measurements on the haemopoiedic systems of SAS/4, 3H and RFM mice have been used to calculate the distribution and population sizes of haemopoietic cells in the bone marrow, spleen and peripheral blood. Considerable strain differences have been found in the sizes of haemopioetic stem cell populations and in the distribution of these cells within the haemopioetic system, although the incidence of CFU-S was shown to be uniform throughout skeletal sites of haemopioesis. The seeding efficiency of colony forming cells in the spleen, measured by the 'f\" factor 24 hours after irradiation and transplantation, also differs between strains and between the bone marrow and spleen of the strains investigated."}
{"id": "PMID:1098923", "title": "Children born to women denied abortion.", "content": "'Compulsory childbearing has varied and sometimes unfavorable consequences for the subsequent life of the child.... The higher incidence of illness and hospitalization..., slightly poorer school marks and performance, somewhat worse integrationin the peer group--all point to a higher risk situation for the (unwanted) child and the family, as well as for society.... Boys born from unwanted pregnancies are more endangered in the development of their personalities than girls'.", "contents": "Children born to women denied abortion. 'Compulsory childbearing has varied and sometimes unfavorable consequences for the subsequent life of the child.... The higher incidence of illness and hospitalization..., slightly poorer school marks and performance, somewhat worse integrationin the peer group--all point to a higher risk situation for the (unwanted) child and the family, as well as for society.... Boys born from unwanted pregnancies are more endangered in the development of their personalities than girls'."}
{"id": "PMID:1098938", "title": "Physical dependence on alcohol in mice.", "content": "Although it has not yet been possible to construct a complete model of alcoholism in experimental animals; some aspects of the disease can now be studied with satisfactory laboratory systems. Production of physical dependence requires a period of continuous intoxication; brief intervals of sobriety allow the accrued dependence to disappear. Administration of ethanol by inhalation, with daily injections of pyrazole, allows maintenance of stable blood alcohol levels in mice. In this model, physical dependence arises to its maximum in a week or two and can decay in less than a day. Sedative drugs suppress the mouse withdrawal reactions but drugs that intefere with catecholamine or gamma-aminobutyric acid pathways facilitate withdrawal seizures. Susceptibility to withdrawal seizures is controlled in part by genetic factors.", "contents": "Physical dependence on alcohol in mice. Although it has not yet been possible to construct a complete model of alcoholism in experimental animals; some aspects of the disease can now be studied with satisfactory laboratory systems. Production of physical dependence requires a period of continuous intoxication; brief intervals of sobriety allow the accrued dependence to disappear. Administration of ethanol by inhalation, with daily injections of pyrazole, allows maintenance of stable blood alcohol levels in mice. In this model, physical dependence arises to its maximum in a week or two and can decay in less than a day. Sedative drugs suppress the mouse withdrawal reactions but drugs that intefere with catecholamine or gamma-aminobutyric acid pathways facilitate withdrawal seizures. Susceptibility to withdrawal seizures is controlled in part by genetic factors."}
{"id": "PMID:1098939", "title": "Direct effects of ethanol on the nervous system.", "content": "Neurophysiological, neurochemical and behavioral studies of the effects of ethanol on the nervous system have so far failed to identify specific, direct, primary mechnisms of action that may account for the typical pattern of alcohol intoxication in vivo. Electroencephalogram and evoked response studies indicate biphasic effects in the intact subject, which may correlate better with the level of arousal than with a specific drug action. Effects on spinal reflexes are also biphasic, probably representing the net result of direct influence on resting membrane potential, primary afferent depolarization, and neurotransmitter release. With the exception of its inhibitory effect on release of oxytocin, vasopressin and possibly other hypothalamic peptides, ethanol does not appear notably different in its spectrum of effects from a wide range of other hypnotics, anesthetics and minor tranquilizers. Interpretation of the findings is complicated by the fact that functional alteration of any given neuronal system by ethanol in vivo may reflect a) direct local action of ethanol on the cells under study, b) change in the input to those cells because of an action elsewhere in the nervous system, c) effects of ethanol metabolites, or d) indirect consequences of decreased blood flow, oxygen or metabolite supply, hormonal action, or hypothermia, due to disturbances of homeostasis in the whole body as a result of deep intoxication. To date, attempts to circmvent b, c and d by the study of brain tissue in vitro have shown consistent effects of ethanol only at concentrations well above those that are meaningful in vivo. Relatively specific patterns of action of different drugs in vivo may prove to be largely dependent on their customary rates and routes of administration, and on summation of minor differences in the dose-response curves with different types of neuron, even though the basic types of molecular action may be essentially similar.", "contents": "Direct effects of ethanol on the nervous system. Neurophysiological, neurochemical and behavioral studies of the effects of ethanol on the nervous system have so far failed to identify specific, direct, primary mechnisms of action that may account for the typical pattern of alcohol intoxication in vivo. Electroencephalogram and evoked response studies indicate biphasic effects in the intact subject, which may correlate better with the level of arousal than with a specific drug action. Effects on spinal reflexes are also biphasic, probably representing the net result of direct influence on resting membrane potential, primary afferent depolarization, and neurotransmitter release. With the exception of its inhibitory effect on release of oxytocin, vasopressin and possibly other hypothalamic peptides, ethanol does not appear notably different in its spectrum of effects from a wide range of other hypnotics, anesthetics and minor tranquilizers. Interpretation of the findings is complicated by the fact that functional alteration of any given neuronal system by ethanol in vivo may reflect a) direct local action of ethanol on the cells under study, b) change in the input to those cells because of an action elsewhere in the nervous system, c) effects of ethanol metabolites, or d) indirect consequences of decreased blood flow, oxygen or metabolite supply, hormonal action, or hypothermia, due to disturbances of homeostasis in the whole body as a result of deep intoxication. To date, attempts to circmvent b, c and d by the study of brain tissue in vitro have shown consistent effects of ethanol only at concentrations well above those that are meaningful in vivo. Relatively specific patterns of action of different drugs in vivo may prove to be largely dependent on their customary rates and routes of administration, and on summation of minor differences in the dose-response curves with different types of neuron, even though the basic types of molecular action may be essentially similar."}
{"id": "PMID:1098940", "title": "Ethanol and brain ribosomes.", "content": "The effects of chronic ethanol intake by mice and rats have been determined on brain ribosomes. Under conditions of ethanol administration, when physical dependence on ethanol either does or does not develop in rats, significant inhibition of polypeptide synthesis was observed in comparison with animals not receiving ethanol. In both rats and mice drinking a 10% ethanol solution, in vitro protein synthesis was found to be diminished more in the free than membrane-bound polyribosomes (polysomes) when compared to similar fractions obtained from control animals. Addition of exogenous amino acids stimulated protein synthesis of free polysomes to a greater extent than that of bound polysomes obtained from both groups of micemin vivo incorporation of [5-3H]-orotic acid into RNA was more inhibited in polysomes than ribosomes of ethanol-drinking mice, suggesting that ethanol affects messenger RNA. In addition, chronic ethanol ingestion produced a decreased in vivo incorporation in ribosomal RNA of a mixed population of ribosomes and polysomes. Chronic ethanol ingestion by mice led also to a differential effect on the polysomal population of the brain. It decreased the amount and the incorporation of precursor label into RNA of free polysomes while exerting an opposite effect on bound polysomes. The significance of these findings relative to brain metabolism is discussed.", "contents": "Ethanol and brain ribosomes. The effects of chronic ethanol intake by mice and rats have been determined on brain ribosomes. Under conditions of ethanol administration, when physical dependence on ethanol either does or does not develop in rats, significant inhibition of polypeptide synthesis was observed in comparison with animals not receiving ethanol. In both rats and mice drinking a 10% ethanol solution, in vitro protein synthesis was found to be diminished more in the free than membrane-bound polyribosomes (polysomes) when compared to similar fractions obtained from control animals. Addition of exogenous amino acids stimulated protein synthesis of free polysomes to a greater extent than that of bound polysomes obtained from both groups of micemin vivo incorporation of [5-3H]-orotic acid into RNA was more inhibited in polysomes than ribosomes of ethanol-drinking mice, suggesting that ethanol affects messenger RNA. In addition, chronic ethanol ingestion produced a decreased in vivo incorporation in ribosomal RNA of a mixed population of ribosomes and polysomes. Chronic ethanol ingestion by mice led also to a differential effect on the polysomal population of the brain. It decreased the amount and the incorporation of precursor label into RNA of free polysomes while exerting an opposite effect on bound polysomes. The significance of these findings relative to brain metabolism is discussed."}
{"id": "PMID:1098941", "title": "Supersensitivity-like phenomena in the central nervous system.", "content": "The are numerous supersensitivity-like changes in the central nervous system associated with denervation and disuse. There is little evidence, however, for increases in postjunctional sensitivity exactly parallel to that which occurs in disused effector organs. As we learn more about the mechanisms responsible for supersensitivity in effector organs, it becomes evident that the operation of such mechanisms in neurons may have reverberations in all spheres of cellular function, including transmitter release and energy control as well as receptor function.", "contents": "Supersensitivity-like phenomena in the central nervous system. The are numerous supersensitivity-like changes in the central nervous system associated with denervation and disuse. There is little evidence, however, for increases in postjunctional sensitivity exactly parallel to that which occurs in disused effector organs. As we learn more about the mechanisms responsible for supersensitivity in effector organs, it becomes evident that the operation of such mechanisms in neurons may have reverberations in all spheres of cellular function, including transmitter release and energy control as well as receptor function."}
{"id": "PMID:1098942", "title": "Use of synthetic luteinizing hormone-releasing hormone in induction of ovulation in amenorrheic patients.", "content": "Luteinizing hormone-releasing hormone (LH-RH), administered to normal women in divided intrramuscular doses or by intravenous infusion, resulted in a prolonged release of luteinizing hormone and follicle-stimulating hormone from the pituitary gland, reliably resulting in ovulation during the late follicular phase. LH-RH was administered 6 days after clomiphene to 18 amenorrheic, anovulatory, infertile women in whom clomiphene therapy had failed previously. When LH-RH was administered intramuscularly in two divided doses, ovulation occurred in six of eight women and in 11 of 22 woman-months of stimulation (50%). One pregnancy occurred. When LH-RH was administered by intravenous infusion, ovulation occurred in nine of ten women and in 16 of 26 woman-months of stimulation (61.5%); three of the ten women became pregnant. Pregnancy also followed treatment withLH-RH in one patient with irregular menses and functional sterility.", "contents": "Use of synthetic luteinizing hormone-releasing hormone in induction of ovulation in amenorrheic patients. Luteinizing hormone-releasing hormone (LH-RH), administered to normal women in divided intrramuscular doses or by intravenous infusion, resulted in a prolonged release of luteinizing hormone and follicle-stimulating hormone from the pituitary gland, reliably resulting in ovulation during the late follicular phase. LH-RH was administered 6 days after clomiphene to 18 amenorrheic, anovulatory, infertile women in whom clomiphene therapy had failed previously. When LH-RH was administered intramuscularly in two divided doses, ovulation occurred in six of eight women and in 11 of 22 woman-months of stimulation (50%). One pregnancy occurred. When LH-RH was administered by intravenous infusion, ovulation occurred in nine of ten women and in 16 of 26 woman-months of stimulation (61.5%); three of the ten women became pregnant. Pregnancy also followed treatment withLH-RH in one patient with irregular menses and functional sterility."}
{"id": "PMID:1098943", "title": "The presence of peptidases in the rabbit hypothalamus capable of inactivating luteinizing hormone-releasing hormone.", "content": "The presence of peptidases in the rabbit hypothalamus capable of inactivating luteinizing hormone-releasing hormone (LH-RH) was investigated with the use of a sensitive and specific radioimmunoassay for the releasing hormone. Enzymes acting on LH-RH were found in two fractions from rabbit hypothalami, a particulate fraction and a supernatant fraction, which very rapidly inactivated the decapeptide. Peptidase activity in the particulate fraction from male and female rabbits was approximately the same, but the supernatant fraction from female animals had significantly greater peptidase activity than that from male animals. These findings confirm the hypothesis that rabbit hypothalamus contains peptidases which inactivate LH-RH, and give some indication that the enzymes may be involved in the control by the central nervous system of reproductive function.", "contents": "The presence of peptidases in the rabbit hypothalamus capable of inactivating luteinizing hormone-releasing hormone. The presence of peptidases in the rabbit hypothalamus capable of inactivating luteinizing hormone-releasing hormone (LH-RH) was investigated with the use of a sensitive and specific radioimmunoassay for the releasing hormone. Enzymes acting on LH-RH were found in two fractions from rabbit hypothalami, a particulate fraction and a supernatant fraction, which very rapidly inactivated the decapeptide. Peptidase activity in the particulate fraction from male and female rabbits was approximately the same, but the supernatant fraction from female animals had significantly greater peptidase activity than that from male animals. These findings confirm the hypothesis that rabbit hypothalamus contains peptidases which inactivate LH-RH, and give some indication that the enzymes may be involved in the control by the central nervous system of reproductive function."}
{"id": "PMID:1098945", "title": "Psoriasis: a review. II.", "content": "Notwithstanding the intense interest in systemic cytotoxic therapy in recent years, the great majority in psoriatics are likely to go on needing topical therapy with tar, dithranol or corticosteroids, and a welcome trend is the spreading of interest in day care centres such as those in Paris (GRUPPER), Stockholm (THYRESSON), Stanford (FARBER) and San Francisco (CRAM) evolving from the out-patient regimes advocated by INGRAM in Leeds a generation ago.", "contents": "Psoriasis: a review. II. Notwithstanding the intense interest in systemic cytotoxic therapy in recent years, the great majority in psoriatics are likely to go on needing topical therapy with tar, dithranol or corticosteroids, and a welcome trend is the spreading of interest in day care centres such as those in Paris (GRUPPER), Stockholm (THYRESSON), Stanford (FARBER) and San Francisco (CRAM) evolving from the out-patient regimes advocated by INGRAM in Leeds a generation ago."}
{"id": "PMID:1098947", "title": "Effect of glucose and anti-insulin serum on insulin released from adult rat isolated islets of Langerhans in long-term organ culture (fourteen days).", "content": "Isolated islets of Langerhans from adult male rats were cultured in media containing either basal (1 gm./ml.) or high glucose (3 mg./ml.), and the media were changed every two days for fourteen days. The insulin released in each medium was measured by a back-titration radioimmunoassay. Insulin secretion was fairly constant in the basal glucose medium and a consistent three-to-sevenfold increase was observed in the alternated or continuous presence of high glucose. Addition of an excess of anti-insulin serum did not change significantly the basal or stimulated release of insulin. The electron microscopic appearance of the islets after fourteen days of incubation showed well-preserved islets both with or without anti-insulin serum. Anti-insulin serum did not alter the ultrastructural aspects of the islets in either basal or high glucose media.", "contents": "Effect of glucose and anti-insulin serum on insulin released from adult rat isolated islets of Langerhans in long-term organ culture (fourteen days). Isolated islets of Langerhans from adult male rats were cultured in media containing either basal (1 gm./ml.) or high glucose (3 mg./ml.), and the media were changed every two days for fourteen days. The insulin released in each medium was measured by a back-titration radioimmunoassay. Insulin secretion was fairly constant in the basal glucose medium and a consistent three-to-sevenfold increase was observed in the alternated or continuous presence of high glucose. Addition of an excess of anti-insulin serum did not change significantly the basal or stimulated release of insulin. The electron microscopic appearance of the islets after fourteen days of incubation showed well-preserved islets both with or without anti-insulin serum. Anti-insulin serum did not alter the ultrastructural aspects of the islets in either basal or high glucose media."}
{"id": "PMID:1098948", "title": "Effect of lectins on hormone release from isolated rat islets of langerhans.", "content": "Lectins from Agaricus bisporus and Agaricus campestris stimulate insulin and glucagon release from isolated rat islets in the presence of 2 mM glucose. In the case of insulin release, maximal stimulation was observed at lectin concentrations above 58 mug. per milliliter (approximately 1 muM).A. bisporus PHA-B-stimulated insulin release was independent of a source of metabolic energy but was abolished by deuterium oxide. The lectin did not alter islet glucose oxidation to CO2 or incorporation of [3H] leucine into trichloracetic acid-precipitable material nor did it modify rates of insulin secretion induced by 20 mM glucose. None of nine other lectins tested stimulated insulin release, whereas stimulation of fat cell glucose oxidation was a general property of the lectins. Binding of 125I-labeled A. bisporus PHA-B to islets increased with time up to one hour and after attainment of equilibrium was very slowly reversible. Binding was directly proportional to islet number and the estimated Kdiss of the binding reaction was 17 mug per milliliter. The total number of A. bisporus PHA-B binding sites per islet was approximately 2 times 10(10). Binding of A. bisporus PHA-B to the islets and A. bisporus PHA-B-stimulated insulin release were inhibited in parallel by a glycopeptide containing the oligosaccharide receptor for the lectin, suggesting that lectin binding is essential for the expression of insulin-releasing activity. It is proposed that the specific interaction between mushroom lectin and its receptors may lead to conformational changes in the structure of the membranes of the islet A2- and B-cells that facilitate exocytosis.", "contents": "Effect of lectins on hormone release from isolated rat islets of langerhans. Lectins from Agaricus bisporus and Agaricus campestris stimulate insulin and glucagon release from isolated rat islets in the presence of 2 mM glucose. In the case of insulin release, maximal stimulation was observed at lectin concentrations above 58 mug. per milliliter (approximately 1 muM).A. bisporus PHA-B-stimulated insulin release was independent of a source of metabolic energy but was abolished by deuterium oxide. The lectin did not alter islet glucose oxidation to CO2 or incorporation of [3H] leucine into trichloracetic acid-precipitable material nor did it modify rates of insulin secretion induced by 20 mM glucose. None of nine other lectins tested stimulated insulin release, whereas stimulation of fat cell glucose oxidation was a general property of the lectins. Binding of 125I-labeled A. bisporus PHA-B to islets increased with time up to one hour and after attainment of equilibrium was very slowly reversible. Binding was directly proportional to islet number and the estimated Kdiss of the binding reaction was 17 mug per milliliter. The total number of A. bisporus PHA-B binding sites per islet was approximately 2 times 10(10). Binding of A. bisporus PHA-B to the islets and A. bisporus PHA-B-stimulated insulin release were inhibited in parallel by a glycopeptide containing the oligosaccharide receptor for the lectin, suggesting that lectin binding is essential for the expression of insulin-releasing activity. It is proposed that the specific interaction between mushroom lectin and its receptors may lead to conformational changes in the structure of the membranes of the islet A2- and B-cells that facilitate exocytosis."}
{"id": "PMID:1098951", "title": "Effects of cyproheptadine on insulin secretion by isolated perifused rat islets.", "content": "The effects of cyproheptadine on basal and glucose-induced insulin release by isolated rat islets was studied by use of a perifusion system. A forty-five minute preincubation of islets with a medium containing both 34.3 mug./ml. (10(-4) M) cyproheptadine and 1.0 mg./ml. glucose completely abolished the biphasic pattern of increased insulin secretion normally obtained after islets are stimulated with a medium containing 3.0 mg./ml. glucose. In another series of experiments, similar results were obtained when the cyproheptadine and 3.0 mg./ml. glucose were presented together. Here, however, the inhibition of the first phase of insulin secretion did not achieve statistical significance and some recovery of the islets' secretory capacity was observed late during the second phase. In studies designed to investigate the influence of cyproheptadine on basal insulin secretion, no obvious effect was observed. These results are discussed in relation to the species-specific alterations in pancreatic beta-cell morphology that have been reported in rats after the oral administration of cyproheptadine.", "contents": "Effects of cyproheptadine on insulin secretion by isolated perifused rat islets. The effects of cyproheptadine on basal and glucose-induced insulin release by isolated rat islets was studied by use of a perifusion system. A forty-five minute preincubation of islets with a medium containing both 34.3 mug./ml. (10(-4) M) cyproheptadine and 1.0 mg./ml. glucose completely abolished the biphasic pattern of increased insulin secretion normally obtained after islets are stimulated with a medium containing 3.0 mg./ml. glucose. In another series of experiments, similar results were obtained when the cyproheptadine and 3.0 mg./ml. glucose were presented together. Here, however, the inhibition of the first phase of insulin secretion did not achieve statistical significance and some recovery of the islets' secretory capacity was observed late during the second phase. In studies designed to investigate the influence of cyproheptadine on basal insulin secretion, no obvious effect was observed. These results are discussed in relation to the species-specific alterations in pancreatic beta-cell morphology that have been reported in rats after the oral administration of cyproheptadine."}
{"id": "PMID:1098953", "title": "The tropic action of gastro-intestinal hormones.", "content": "There is evidence that under various physiological circumstances long-term adaptation of structure and function of the gut occurs. The mechanisms of these changes are not clear but gastro-intestinal hormones may be involved. In particular, gastrin which has been shown experimentally to stimulate growth and development of parts of the gut, probably has a role in maintaining the structure of the normal upper alimentary tract. Cholecystokinin may be of major importance in producing adaptive changes in the pancreas in response to dietary modifications and enteroglucagon is possibly concerned with maintaining a normal small intestinal structure. The importance of the 'trophic' action of gatrointestinal hormones is becoming more widely recognised and as new gastro-intestinal hormones become established, this aspect of their physiological importance as well as their acute effects, will deserve attention.", "contents": "The tropic action of gastro-intestinal hormones. There is evidence that under various physiological circumstances long-term adaptation of structure and function of the gut occurs. The mechanisms of these changes are not clear but gastro-intestinal hormones may be involved. In particular, gastrin which has been shown experimentally to stimulate growth and development of parts of the gut, probably has a role in maintaining the structure of the normal upper alimentary tract. Cholecystokinin may be of major importance in producing adaptive changes in the pancreas in response to dietary modifications and enteroglucagon is possibly concerned with maintaining a normal small intestinal structure. The importance of the 'trophic' action of gatrointestinal hormones is becoming more widely recognised and as new gastro-intestinal hormones become established, this aspect of their physiological importance as well as their acute effects, will deserve attention."}
{"id": "PMID:1098955", "title": "Hepatic veno-occlusive disease in a renal transplant patient receiving azathioprine.", "content": "Central vein phlebitis and occlusion developed in a patient who was receiving immunosuppressive therapy with azathioprine (Imuran) and prednisone after renal transplantation. He had been well for 1 year before evidence of liver disease was noted; once begun, it progressed to hepatocellular failure in spite of a change in immunosuppressants from azathioprine to cyclophosphamide. Several known causes of venoocclusive disease and the possible causes in this patient are discussed.", "contents": "Hepatic veno-occlusive disease in a renal transplant patient receiving azathioprine. Central vein phlebitis and occlusion developed in a patient who was receiving immunosuppressive therapy with azathioprine (Imuran) and prednisone after renal transplantation. He had been well for 1 year before evidence of liver disease was noted; once begun, it progressed to hepatocellular failure in spite of a change in immunosuppressants from azathioprine to cyclophosphamide. Several known causes of venoocclusive disease and the possible causes in this patient are discussed."}
{"id": "PMID:1098962", "title": "The problems of abdominal operations in elderly patients.", "content": "In a series of 152 patients aged 65 years or older who underwent abdominal operations between January 1 and December 31, 1971, the total mortality was 17 percent. Pulmonary pathologic conditions were responsible for the majority of the deaths. Use of IPPB did not significantly influence the postoperative course. This study suggests that meticulous preoperative preparation of patients; deliberate, clean, and efficient surgical techniques; and intensive postoperative care are of utmost importance in surgical treatment of the elderly.", "contents": "The problems of abdominal operations in elderly patients. In a series of 152 patients aged 65 years or older who underwent abdominal operations between January 1 and December 31, 1971, the total mortality was 17 percent. Pulmonary pathologic conditions were responsible for the majority of the deaths. Use of IPPB did not significantly influence the postoperative course. This study suggests that meticulous preoperative preparation of patients; deliberate, clean, and efficient surgical techniques; and intensive postoperative care are of utmost importance in surgical treatment of the elderly."}
{"id": "PMID:1098968", "title": "Aspects of the effect of metiamide on pentagastrin-stimulated and basal gastric secretion of acid and pepsin in man.", "content": "This study has examined the inhibition produced by metiamide on the gastric secretion of acid and pepsin in 13 patients with duodenal and three with gastric ulcer. The effect of metiamide on the response to a range of doses of pentagastrin in three normal individuals was determined, as was the interaction of metiamide and atropine on prolonged basal secretion. Metiamide inhibited the secretion of acid more than pepsin and the gastric secretion of patients with gastric ulcer more than duodenal ulcer. Metiamide inhibited both the maximal secretory response attainable with pentagastrin and decreased the sensitivity to pentagastrin. Atropine augmented and prolonged the action of metiamide.", "contents": "Aspects of the effect of metiamide on pentagastrin-stimulated and basal gastric secretion of acid and pepsin in man. This study has examined the inhibition produced by metiamide on the gastric secretion of acid and pepsin in 13 patients with duodenal and three with gastric ulcer. The effect of metiamide on the response to a range of doses of pentagastrin in three normal individuals was determined, as was the interaction of metiamide and atropine on prolonged basal secretion. Metiamide inhibited the secretion of acid more than pepsin and the gastric secretion of patients with gastric ulcer more than duodenal ulcer. Metiamide inhibited both the maximal secretory response attainable with pentagastrin and decreased the sensitivity to pentagastrin. Atropine augmented and prolonged the action of metiamide."}
{"id": "PMID:1098980", "title": "Development of host resistance to Fasciola hepatica after the elimination of primary infection with diamphenethide.", "content": "We studied the specificity of the individual developmental stages of Fasciola hepatica for evoking immune response of the host to reinfection with this parasite, whereby the primary infection was eliminated by a dose of 150 mg/kg diamphenethide administered in various intervals. In rats we observed a state of hypersensitivity demonstrated by retarded migration and growth of the flukes and the reduction in the number of sexually developed parasites. The changes were most marked, if the elimination of the immunizing infection followed 8-10 weeks after primary infection.", "contents": "Development of host resistance to Fasciola hepatica after the elimination of primary infection with diamphenethide. We studied the specificity of the individual developmental stages of Fasciola hepatica for evoking immune response of the host to reinfection with this parasite, whereby the primary infection was eliminated by a dose of 150 mg/kg diamphenethide administered in various intervals. In rats we observed a state of hypersensitivity demonstrated by retarded migration and growth of the flukes and the reduction in the number of sexually developed parasites. The changes were most marked, if the elimination of the immunizing infection followed 8-10 weeks after primary infection."}
{"id": "PMID:1098982", "title": "Effects of various cerebral metabolic activators on glucose metabolism of brain.", "content": "The effects of various metabolic activators on the glucose metabolism of the perfused cat brain have been investigated.  (1) When the perfusion is done with the blood containing cytidine monophosphate, the glucose metabolism of the brain is enhanced, as compared to the perfusion with the blood without cytidine monophosphate.  There is no marked change in the cerebral metabolic rate and in the contents of intermediate metabolite of glucose in the brain.  (2) Citicoline enhances the incorporation of blood glucose into the brain and its metabolism in the brain.  It increases slightly the cerebral blood flow rate and decreases the accumulation of lactate in the brain.  (3) Either Pyrithioxin or Meclophenoxate has no effect on the glucose metabolism of the brain nor on the cerebral metabolic rate.", "contents": "Effects of various cerebral metabolic activators on glucose metabolism of brain. The effects of various metabolic activators on the glucose metabolism of the perfused cat brain have been investigated.  (1) When the perfusion is done with the blood containing cytidine monophosphate, the glucose metabolism of the brain is enhanced, as compared to the perfusion with the blood without cytidine monophosphate.  There is no marked change in the cerebral metabolic rate and in the contents of intermediate metabolite of glucose in the brain.  (2) Citicoline enhances the incorporation of blood glucose into the brain and its metabolism in the brain.  It increases slightly the cerebral blood flow rate and decreases the accumulation of lactate in the brain.  (3) Either Pyrithioxin or Meclophenoxate has no effect on the glucose metabolism of the brain nor on the cerebral metabolic rate."}
{"id": "PMID:1098985", "title": "[Symptom-centered group therapy for phobias--the problem-oriented workshop in psychotherapy].", "content": "Short term psychotherapy is increasingly recognized as a socioeconomic necessity. Problem-oriented treatments, particularly if applied in problem-homogenous groups, are one means of economizing and sometimes even improving psychotherapy. They also require a re-evaluation of the nature of the patient-therapist dyad. The \"task-oriented small group\" as a basic field of human interaction has been studied intensively in experimental social psychology. Nevertheless, group psychotherapy and behavior therapy have largely neglected group-specific aspects of such variables as cohesion, task-orientation, modelling, mutual self-help, risk-taking, motivation and insight. This paper outlines some recent changes in this respect, and discusses in particular the \"symptomoriented peer workshop\" as a treatment milieu. The general therapeutic potential of the peer workshop is briefly assessed with regard to the application of the above mentioned group variables in peer self-help groups, rehabilitation programs for the chronically hospitalized, symptom or depth-treatment in short-term psychotherapy, and in re-education programs for problem-children. The main concern is with its specific application to the treatment of phobias. Phobias are comparatively easy to define, yet are often connected with other personality problems. This has made them a model-problem in the arguments about symptom-substitution between \"psycho\"- and \"behavior\" -therapists, and it has stimulated discussion as to how behavior-focused treatments affect cognition and emotion, and to what extend depth and insight-oriented therapies change behavior. The interaction between treated and non-treated problems, and the behavioral, cognitive and emotional effects of differential treatment ingredients are discussed with special reference to the first controlled study in behavior therapy, that applied behavior-focussed confrontation with reality (flooding in vivo) under the sociodynamics of the small gsoup. Problem-focussed retraining under real-life conditions (reality testing) as group-therapeutic fieldwork can be a means of treatment in its own right may open up the patient for subsequent attempts to tackle more complex problems, or may just be an adjuvant in a multi-level approach. This certainly applies for phobias and can probably be generalized to other problems.", "contents": "[Symptom-centered group therapy for phobias--the problem-oriented workshop in psychotherapy]. Short term psychotherapy is increasingly recognized as a socioeconomic necessity. Problem-oriented treatments, particularly if applied in problem-homogenous groups, are one means of economizing and sometimes even improving psychotherapy. They also require a re-evaluation of the nature of the patient-therapist dyad. The \"task-oriented small group\" as a basic field of human interaction has been studied intensively in experimental social psychology. Nevertheless, group psychotherapy and behavior therapy have largely neglected group-specific aspects of such variables as cohesion, task-orientation, modelling, mutual self-help, risk-taking, motivation and insight. This paper outlines some recent changes in this respect, and discusses in particular the \"symptomoriented peer workshop\" as a treatment milieu. The general therapeutic potential of the peer workshop is briefly assessed with regard to the application of the above mentioned group variables in peer self-help groups, rehabilitation programs for the chronically hospitalized, symptom or depth-treatment in short-term psychotherapy, and in re-education programs for problem-children. The main concern is with its specific application to the treatment of phobias. Phobias are comparatively easy to define, yet are often connected with other personality problems. This has made them a model-problem in the arguments about symptom-substitution between \"psycho\"- and \"behavior\" -therapists, and it has stimulated discussion as to how behavior-focused treatments affect cognition and emotion, and to what extend depth and insight-oriented therapies change behavior. The interaction between treated and non-treated problems, and the behavioral, cognitive and emotional effects of differential treatment ingredients are discussed with special reference to the first controlled study in behavior therapy, that applied behavior-focussed confrontation with reality (flooding in vivo) under the sociodynamics of the small gsoup. Problem-focussed retraining under real-life conditions (reality testing) as group-therapeutic fieldwork can be a means of treatment in its own right may open up the patient for subsequent attempts to tackle more complex problems, or may just be an adjuvant in a multi-level approach. This certainly applies for phobias and can probably be generalized to other problems."}
{"id": "PMID:1098990", "title": "Induction of pseudopregnancy in the rat by electrochemical stimulation of the brain.", "content": "Electrochemical stimulation of the neocortex, VMH-arcuate hypothalamus, medial preoptic area, corticomedial amygdala, and dorsal hippocampus was performed under pentobarbital anesthesia to determine the brain regions involved in prolactin release as evidenced by pseudopregnancy. Stimulation occurred on the day of vaginal estrus, followed 4 days later by uterine traumatization. All animals were sacrificed 8 days after brain stimulation. Medial preoptic sitmulation resulted in an 83% incidence of pseudopregnancy while VMH-arcuate stimulation resulted in 43% incidence. Amygdaloid and hippocampal stimulation resulted in a lower incidence of pseudopregnancy (20 and 24%, respectively). Stimulation of the neocortex or sham stimulation of the five brain regions did not induce pseudopregnancy. These data indicate that the MPOA and VMH-arcuate hypothalamus are areas which may facilitate the release of prolactin necessary for the development of pseudopregnancy. The lower incidence of deciduomata formation resulting from electrochemical stimulation on the amygdala and hippocampus suggest that these areas have a minor effect on stimulating the induction of pseudopregnancy.", "contents": "Induction of pseudopregnancy in the rat by electrochemical stimulation of the brain. Electrochemical stimulation of the neocortex, VMH-arcuate hypothalamus, medial preoptic area, corticomedial amygdala, and dorsal hippocampus was performed under pentobarbital anesthesia to determine the brain regions involved in prolactin release as evidenced by pseudopregnancy. Stimulation occurred on the day of vaginal estrus, followed 4 days later by uterine traumatization. All animals were sacrificed 8 days after brain stimulation. Medial preoptic sitmulation resulted in an 83% incidence of pseudopregnancy while VMH-arcuate stimulation resulted in 43% incidence. Amygdaloid and hippocampal stimulation resulted in a lower incidence of pseudopregnancy (20 and 24%, respectively). Stimulation of the neocortex or sham stimulation of the five brain regions did not induce pseudopregnancy. These data indicate that the MPOA and VMH-arcuate hypothalamus are areas which may facilitate the release of prolactin necessary for the development of pseudopregnancy. The lower incidence of deciduomata formation resulting from electrochemical stimulation on the amygdala and hippocampus suggest that these areas have a minor effect on stimulating the induction of pseudopregnancy."}
{"id": "PMID:1098991", "title": "Twenty-five years of hospital and community psychiatry: 1945-1970.", "content": "The author, the first medical director of the American Psychiatric Association and APA president in 1964-65, presents a historical review of the 25 years between 1945 and 1970, a period in which there was a reawakening of interest in the mentally ill and mentally retarded after years of neglect. He lists major landmarks in mental health care during those years, describes the first Mental Hospital Institiute held by the American Psychiatric Association in 1949, and presents an anecdotal footnote about the Joint Commission on Mental Illness and Health.", "contents": "Twenty-five years of hospital and community psychiatry: 1945-1970. The author, the first medical director of the American Psychiatric Association and APA president in 1964-65, presents a historical review of the 25 years between 1945 and 1970, a period in which there was a reawakening of interest in the mentally ill and mentally retarded after years of neglect. He lists major landmarks in mental health care during those years, describes the first Mental Hospital Institiute held by the American Psychiatric Association in 1949, and presents an anecdotal footnote about the Joint Commission on Mental Illness and Health."}
{"id": "PMID:1098999", "title": "Further characterization of antigen-dependent migration inhibition factor in mice.", "content": "Antigen-dependent MIF was produced in inbred mice. Lymph node cells from mice sensitized to tubercle bacilli were incubated with small quantities of antigen. The supernatant contained antigen-dependent MIF and its activity when tested on mouse and rabbit spleen was minimal unless PPD was added to the supernatant. The production of the antigen-dependent MIF was T cell-dependent, as shown by the use of anti-theta serum. Its molecular weight was in the range of 50--100,000 and it was concluded that the factor is different from conventional antibodies or antigen-antibody complexes.", "contents": "Further characterization of antigen-dependent migration inhibition factor in mice. Antigen-dependent MIF was produced in inbred mice. Lymph node cells from mice sensitized to tubercle bacilli were incubated with small quantities of antigen. The supernatant contained antigen-dependent MIF and its activity when tested on mouse and rabbit spleen was minimal unless PPD was added to the supernatant. The production of the antigen-dependent MIF was T cell-dependent, as shown by the use of anti-theta serum. Its molecular weight was in the range of 50--100,000 and it was concluded that the factor is different from conventional antibodies or antigen-antibody complexes."}
{"id": "PMID:1099000", "title": "The recovery of the B-cell population in adult thymectomized, lethally irradiated and bone marrow-reconstituted mice.", "content": "The recovery of the B-cell population in adult thymectomized, irradiated and bone marrow-reconstituted mice (T X BM mice was estimated at various times after bone marrow transplantation. The spleen cells to be tested were mixed with dexamethasone-resistant thymocytes (DRT) and sheep red blood cells (SRBC) and transferrred to irradiated recipients. The number of plaque-forming cells (PFC) in the spleen of the recipients was determined 7 days later. Using this functional B-cell assay a sequential appearance of the precursors of IgM-, IgG- and IgA-PFC in the spleen of T X BM mice was observed. The precursors of IgM-PFG (IgM-B cells) were present immediately after transplantation. The first IgG-B cells could be detected at 13-16 days after transplantation and the IgA-B cells finally appeared at 22 days after transplantation. The number of B cells reached a constant and normal level at 30 days after transplantation. The IgM-, IgG- and IgA-B cell development in sham-thymectomized, irradiated and bone narrow-reconstituted mice (ST X BM mice) was virtually the same as in T X BM mice.", "contents": "The recovery of the B-cell population in adult thymectomized, lethally irradiated and bone marrow-reconstituted mice. The recovery of the B-cell population in adult thymectomized, irradiated and bone marrow-reconstituted mice (T X BM mice was estimated at various times after bone marrow transplantation. The spleen cells to be tested were mixed with dexamethasone-resistant thymocytes (DRT) and sheep red blood cells (SRBC) and transferrred to irradiated recipients. The number of plaque-forming cells (PFC) in the spleen of the recipients was determined 7 days later. Using this functional B-cell assay a sequential appearance of the precursors of IgM-, IgG- and IgA-PFC in the spleen of T X BM mice was observed. The precursors of IgM-PFG (IgM-B cells) were present immediately after transplantation. The first IgG-B cells could be detected at 13-16 days after transplantation and the IgA-B cells finally appeared at 22 days after transplantation. The number of B cells reached a constant and normal level at 30 days after transplantation. The IgM-, IgG- and IgA-B cell development in sham-thymectomized, irradiated and bone narrow-reconstituted mice (ST X BM mice) was virtually the same as in T X BM mice."}
{"id": "PMID:1099004", "title": "Levels of lysosomal enzymes in tissues of mice infected with Mycoplasma fermentans.", "content": "The effects of a toxic dose of Mycoplasma fermentans on levels of lysosomal enzymes in mice were examined. Washed cell suspensions (approximately 10(10) colony-forming units) of a recent isolate of M. fermentans were injected intraperitoneally into 3- to 4-week-old BALB mice, and levels of acid phosphatase and beta-glucuronidase were monitored in liver, spleen, thymus, and serum. Levels of acid phosphatase remained essentially normal, but levels of beta-glucuronidase were markedly evevated in serum and to a lesser extent in liver and thymus. The peak response of serum beta-glucuronidase was noted at 8 h postinjection, with a level of 30 mug of phenolphthalein released per ml per h, representing a six-fold increase over control levels. Pretreatment with BCG did not potentiate the effect as it did with endotoxin. The implication of this increased lysosomal enzyme activity in \"lethal toxicity\" is that that the increase may be secondary to some other cytotoxic event, or that the affinity of mycoplasmas for biological membranes may be involved. The data suggest that the role of lysosomal enzymes in other models of mycoplasma-induced disease should be evaluated.", "contents": "Levels of lysosomal enzymes in tissues of mice infected with Mycoplasma fermentans. The effects of a toxic dose of Mycoplasma fermentans on levels of lysosomal enzymes in mice were examined. Washed cell suspensions (approximately 10(10) colony-forming units) of a recent isolate of M. fermentans were injected intraperitoneally into 3- to 4-week-old BALB mice, and levels of acid phosphatase and beta-glucuronidase were monitored in liver, spleen, thymus, and serum. Levels of acid phosphatase remained essentially normal, but levels of beta-glucuronidase were markedly evevated in serum and to a lesser extent in liver and thymus. The peak response of serum beta-glucuronidase was noted at 8 h postinjection, with a level of 30 mug of phenolphthalein released per ml per h, representing a six-fold increase over control levels. Pretreatment with BCG did not potentiate the effect as it did with endotoxin. The implication of this increased lysosomal enzyme activity in \"lethal toxicity\" is that that the increase may be secondary to some other cytotoxic event, or that the affinity of mycoplasmas for biological membranes may be involved. The data suggest that the role of lysosomal enzymes in other models of mycoplasma-induced disease should be evaluated."}
{"id": "PMID:1099005", "title": "Sequential appearance of salivary antibodies after oral immunization of axenic mice.", "content": "The purpose of the present investigation was to examine the production of specific salivary antibodies after oral immunization of axenic mice (ICR/CD-1) with formalinized Escherichia coli (ATCC 11775). Three aspects of the humoral response were studied: (i) the sequential appearance of anti-E. coli antibodies; (ii) classes of antibodies produced in response to the antigen; and (iii) antibody specificity. Antibody levels were determined by passive hemagglutination with soluble lipoplysaccharide and bacterial agglutination, using preparations of somatic O and flagellar H antigens. A short latent period of 1 to 3 days was observed when saliva samples were assayed for anti-lipopolysaccharide and anti-O antibodies. The salivary antibody titers peaked at 11 days after initiation of the immunization regimen. Only two of six mice exhibited a change in serum antibody levels against these antigens. The predominant class of early antibody in saliva was immunoglobulin (Ig)G; however by 7 days IgA WAS found to comprise the major portion of specific immunoglobulins. Serum antibodies directed against falgellar antigenic determinants were primarily of the IgM class, whereas the salivary antibodies were IgG in nature.", "contents": "Sequential appearance of salivary antibodies after oral immunization of axenic mice. The purpose of the present investigation was to examine the production of specific salivary antibodies after oral immunization of axenic mice (ICR/CD-1) with formalinized Escherichia coli (ATCC 11775). Three aspects of the humoral response were studied: (i) the sequential appearance of anti-E. coli antibodies; (ii) classes of antibodies produced in response to the antigen; and (iii) antibody specificity. Antibody levels were determined by passive hemagglutination with soluble lipoplysaccharide and bacterial agglutination, using preparations of somatic O and flagellar H antigens. A short latent period of 1 to 3 days was observed when saliva samples were assayed for anti-lipopolysaccharide and anti-O antibodies. The salivary antibody titers peaked at 11 days after initiation of the immunization regimen. Only two of six mice exhibited a change in serum antibody levels against these antigens. The predominant class of early antibody in saliva was immunoglobulin (Ig)G; however by 7 days IgA WAS found to comprise the major portion of specific immunoglobulins. Serum antibodies directed against falgellar antigenic determinants were primarily of the IgM class, whereas the salivary antibodies were IgG in nature."}
{"id": "PMID:1099006", "title": "In situ separation of bacterial trapping and killing functions of the perfused liver.", "content": "CF-1 mice cleared and killed 80% of a 1.2 x 10(9) intravenous dose of Salmonella typhimurium after 30 min. The perfused mouse liver trapped 70% of a similar dose of S. typhimurium in a single pass, but in the perfusion model no significant killing of the trapped organisms was observed. The perfused rat liver also avidly trapped bacteria. Because of its larger size, we have been able to devise techniques to experimentally distinguish between the bacterial trapping and killing functions of this organ. When the liver was washed free of blood with sterile M-199, over 70 to 80% of a 10(6) to 10(10) dose of viable S. typhimurium was trapped after a single pass, but no significant bacterial killing was observed. When blood or plasma was added to the perfusion medium, over 50% of the trapped bacteria were killed in 15 to 30 min. Phase contrast and electron micrographs of perfused livers showed extensive extracellular trapping of bacteria in the sinusoids. Our data show that humoral factors are apparently not necessary for efficient trapping of live Salmonella by the perfused rat liver but are an absolute requirement for bacterial activity of the organ.", "contents": "In situ separation of bacterial trapping and killing functions of the perfused liver. CF-1 mice cleared and killed 80% of a 1.2 x 10(9) intravenous dose of Salmonella typhimurium after 30 min. The perfused mouse liver trapped 70% of a similar dose of S. typhimurium in a single pass, but in the perfusion model no significant killing of the trapped organisms was observed. The perfused rat liver also avidly trapped bacteria. Because of its larger size, we have been able to devise techniques to experimentally distinguish between the bacterial trapping and killing functions of this organ. When the liver was washed free of blood with sterile M-199, over 70 to 80% of a 10(6) to 10(10) dose of viable S. typhimurium was trapped after a single pass, but no significant bacterial killing was observed. When blood or plasma was added to the perfusion medium, over 50% of the trapped bacteria were killed in 15 to 30 min. Phase contrast and electron micrographs of perfused livers showed extensive extracellular trapping of bacteria in the sinusoids. Our data show that humoral factors are apparently not necessary for efficient trapping of live Salmonella by the perfused rat liver but are an absolute requirement for bacterial activity of the organ."}
{"id": "PMID:1099007", "title": "The F\u00e9d\u00e9ration Dentaire Internationale, 1900-1975.", "content": "The author presents an historical survey of the development of the F\u00e9d\u00e9ration Dentaire Internationale. Beginning with its foundation by Charles Godon in 1900 he describes the growth of the Federation as an organization of the dental profession which continued despite the interruption of two world wars. The most important aims and events of the first 50 years are presented in chronological sequence. In the period of the last 25 years--after the London Congress of 1952---the main emphasis is placed on describing the present structure and objectives of the Federation. Brief reflections on the Federation of today conclude the article.", "contents": "The F\u00e9d\u00e9ration Dentaire Internationale, 1900-1975. The author presents an historical survey of the development of the F\u00e9d\u00e9ration Dentaire Internationale. Beginning with its foundation by Charles Godon in 1900 he describes the growth of the Federation as an organization of the dental profession which continued despite the interruption of two world wars. The most important aims and events of the first 50 years are presented in chronological sequence. In the period of the last 25 years--after the London Congress of 1952---the main emphasis is placed on describing the present structure and objectives of the Federation. Brief reflections on the Federation of today conclude the article."}
{"id": "PMID:1099008", "title": "The mystery bridges of Martin Bormann's alleged Berlin skull--key clues for forensic identification or another Piltdown case?", "content": "Dental records prepared by Martin Bormann's dentist Dr Hugo Blaschke in 1945 have been examined in the Captured Military Record Branch of the US National Archives in 1972 and compared with the direct evidence from examination of skeletal remains unearthed in Berlin in 1972 at a site next to Lehrter RR station where Bormann is known to have been seen alive for the last time on May 2, 1945. There is adequate qualitative and quantitative concurrence between all available antemortem and postmortem dental data to ascertain that the forensic identification of Martin Bormann can be considered established.", "contents": "The mystery bridges of Martin Bormann's alleged Berlin skull--key clues for forensic identification or another Piltdown case? Dental records prepared by Martin Bormann's dentist Dr Hugo Blaschke in 1945 have been examined in the Captured Military Record Branch of the US National Archives in 1972 and compared with the direct evidence from examination of skeletal remains unearthed in Berlin in 1972 at a site next to Lehrter RR station where Bormann is known to have been seen alive for the last time on May 2, 1945. There is adequate qualitative and quantitative concurrence between all available antemortem and postmortem dental data to ascertain that the forensic identification of Martin Bormann can be considered established."}
{"id": "PMID:1099009", "title": "Lymphokines in sensitized rats. I. Migration inhibitory factor(s) from specifically stimulated thymocytes in vitro.", "content": "Cell-mediated immune responses were studied in Wistar rats by migration inhibition factor (MIF) assays in vitro of lymphoid cells derived from thymus, spleen and peripheral blood and peritoneal exudates and by delayed cutaneous hypersensitivity reactions in vivo. Using PPD and purified diphtheria toxoid as soluble test antigens it appeared that significant migration inhibition was observed only when sensitized thymus and peritoneal exudate cells were used in the direct capillary migration system. Indirect MIF assays, using both capillary and agarose techniques, showed that migration inhibitory activity was found in thymus culture supernatants as well as in spleen culture supernatants of sensitized animals. The MIF activity in thymic cell culture supernatants seemed to be antigen-independent, although in this respect no definitive conclusions can be drawn from the present results. Migration inhibition in direct and indirect techniques always correlated with a positive delayed-type skin reaction. It is suggested that a population of thymus cells of sensitized animals is able to produce MIF on specific antigenic stimulation in vitro. This might support the idea that a cell population within the thymus possesses characteristics of peripheral T cells.", "contents": "Lymphokines in sensitized rats. I. Migration inhibitory factor(s) from specifically stimulated thymocytes in vitro. Cell-mediated immune responses were studied in Wistar rats by migration inhibition factor (MIF) assays in vitro of lymphoid cells derived from thymus, spleen and peripheral blood and peritoneal exudates and by delayed cutaneous hypersensitivity reactions in vivo. Using PPD and purified diphtheria toxoid as soluble test antigens it appeared that significant migration inhibition was observed only when sensitized thymus and peritoneal exudate cells were used in the direct capillary migration system. Indirect MIF assays, using both capillary and agarose techniques, showed that migration inhibitory activity was found in thymus culture supernatants as well as in spleen culture supernatants of sensitized animals. The MIF activity in thymic cell culture supernatants seemed to be antigen-independent, although in this respect no definitive conclusions can be drawn from the present results. Migration inhibition in direct and indirect techniques always correlated with a positive delayed-type skin reaction. It is suggested that a population of thymus cells of sensitized animals is able to produce MIF on specific antigenic stimulation in vitro. This might support the idea that a cell population within the thymus possesses characteristics of peripheral T cells."}
{"id": "PMID:1099010", "title": "Lymphokines. I. Use of insoluble concanavalin A for the production of migration inhibitory factor in guinea pig lymphocyte cultures.", "content": "Incubation of guinea pig lymph node lymphocytes with insoluble concanavalin A (Con A) in serum-free medium yields sizable amounts of migration inhibition (MIF) and mitogenic factors (MF). Various controls confirmed that the activities detected in supernatants from such cultures are not due to free soluble Con A released during culture. Whereas guinea pig MIF does not act upon human and horse monocytes, guinea pig MF stimulated human but not horse lymphocytes. Upon stimulation by insoluble Con A, guinea pig MIF and MF appear to be produced essentially by T lymphocytes. The technique proposed for producing lymphokines appears convenient for further purification and biochemical characterization of culture supernatants.", "contents": "Lymphokines. I. Use of insoluble concanavalin A for the production of migration inhibitory factor in guinea pig lymphocyte cultures. Incubation of guinea pig lymph node lymphocytes with insoluble concanavalin A (Con A) in serum-free medium yields sizable amounts of migration inhibition (MIF) and mitogenic factors (MF). Various controls confirmed that the activities detected in supernatants from such cultures are not due to free soluble Con A released during culture. Whereas guinea pig MIF does not act upon human and horse monocytes, guinea pig MF stimulated human but not horse lymphocytes. Upon stimulation by insoluble Con A, guinea pig MIF and MF appear to be produced essentially by T lymphocytes. The technique proposed for producing lymphokines appears convenient for further purification and biochemical characterization of culture supernatants."}
{"id": "PMID:1099011", "title": "Regulation of antibody formation in chickens escaping from tolerance to human serum albumin.", "content": "The immune capacity of chickens made tolerant to human serum albumin just after hatching was studied after a primary and secondary challenge at 7--14 weeks of age, of either 1 mg/kg or 100 mg/kg. The class and avidity of antibody produced by birds \"escaping\" from tolerance was similar to normal controls. The escaping chickens made a normal peak antibody response to a high dose but not to a low dose (except that the response to a low secondary dose after priming with a high dose was normal); but the decline of the antibody titer was abrupt, indicating inability to maintain the response. It is concluded that the antibody-forming capacity of B cells is normal, but regulation of antibody formation is impaired in previously tolerant chickens.", "contents": "Regulation of antibody formation in chickens escaping from tolerance to human serum albumin. The immune capacity of chickens made tolerant to human serum albumin just after hatching was studied after a primary and secondary challenge at 7--14 weeks of age, of either 1 mg/kg or 100 mg/kg. The class and avidity of antibody produced by birds \"escaping\" from tolerance was similar to normal controls. The escaping chickens made a normal peak antibody response to a high dose but not to a low dose (except that the response to a low secondary dose after priming with a high dose was normal); but the decline of the antibody titer was abrupt, indicating inability to maintain the response. It is concluded that the antibody-forming capacity of B cells is normal, but regulation of antibody formation is impaired in previously tolerant chickens."}
{"id": "PMID:1099012", "title": "Behaviour of local and systemic immunoglobulins in patients with lung cancer.", "content": "Tissue blocks from lung parenchyma in the vicinity of primary lung cancer and regional lymph nodes were collected during surgery from 22 patients. Cryostat sections were assessed for the presence of five five immunoglobulin classes by means of immunofluorescence. Imprints made from surfaces of freshly resected tumours were used for indirect immunofluorescent reaction to detect anti-tumour antibodies in the autologous system sera of patients after surgery. Also, sera of 60 lung cancer patients were assessed for IgG, A, M levels. It has been found that the direct vicinity of primary lung cancer and regional lymph nodes was a site of intensive immunoglobulin synthesis is plasma cells, with a predominance of the IgA and IgG classes. Serum anti-tumour antibody was found in 3 cases out of 22. There were significantly elevated serum immunoglobulin levels with predominance of IgA and IgG. Inoperable patients had higher immunoglobulin levels then those who underwent surgery.", "contents": "Behaviour of local and systemic immunoglobulins in patients with lung cancer. Tissue blocks from lung parenchyma in the vicinity of primary lung cancer and regional lymph nodes were collected during surgery from 22 patients. Cryostat sections were assessed for the presence of five five immunoglobulin classes by means of immunofluorescence. Imprints made from surfaces of freshly resected tumours were used for indirect immunofluorescent reaction to detect anti-tumour antibodies in the autologous system sera of patients after surgery. Also, sera of 60 lung cancer patients were assessed for IgG, A, M levels. It has been found that the direct vicinity of primary lung cancer and regional lymph nodes was a site of intensive immunoglobulin synthesis is plasma cells, with a predominance of the IgA and IgG classes. Serum anti-tumour antibody was found in 3 cases out of 22. There were significantly elevated serum immunoglobulin levels with predominance of IgA and IgG. Inoperable patients had higher immunoglobulin levels then those who underwent surgery."}
{"id": "PMID:1099013", "title": "Yoshida ascites sarcoma grown in mice.", "content": "Xenogeneic Yoshida ascites sarcoma grows well in the abdominal cavity of thymectomized, irradiated mice reconstituted with syngeneic bone marrow. These animals displayed a 100-percent mortality. In control sham-thymectomized, irradiated mice reconstituted with syngeneic bone marrow mortality was significantly lower. The tumor growth could be neutralized by means of single intravenous injection of nonimmune spleen cells inoculated at the same time as the tumor. Tumor cells grown in mice killed normal rats and were rejected by normal mice.", "contents": "Yoshida ascites sarcoma grown in mice. Xenogeneic Yoshida ascites sarcoma grows well in the abdominal cavity of thymectomized, irradiated mice reconstituted with syngeneic bone marrow. These animals displayed a 100-percent mortality. In control sham-thymectomized, irradiated mice reconstituted with syngeneic bone marrow mortality was significantly lower. The tumor growth could be neutralized by means of single intravenous injection of nonimmune spleen cells inoculated at the same time as the tumor. Tumor cells grown in mice killed normal rats and were rejected by normal mice."}
{"id": "PMID:1099014", "title": "Organization and unionization of health workers in the United States: the trade union perspective.", "content": "Recent legislation in Washington opened the door to organization and unionization of health workers in voluntary hospitals and highlighted the poor pay and unorganized status of this enormous work force of at least 1.5 million persons, many of whom are women, black, or Spanish-speaking. The authors, who are senior officials of District 1199 of the National Union of Hospital and Health Care Employees, chart the factors associated with the low priority given over the years to organizing hospital workers, and the first breakthroughs, in New York City hospitals, in 1958. From a start with lowest income hospital workers, the subsequent inclusion of technical and professional workers and linkages with the highly publicized hospital workers' strike in Charleston, South Carolina in 1969. The benefits of unionization and the broader social goals of the union are discussed.", "contents": "Organization and unionization of health workers in the United States: the trade union perspective. Recent legislation in Washington opened the door to organization and unionization of health workers in voluntary hospitals and highlighted the poor pay and unorganized status of this enormous work force of at least 1.5 million persons, many of whom are women, black, or Spanish-speaking. The authors, who are senior officials of District 1199 of the National Union of Hospital and Health Care Employees, chart the factors associated with the low priority given over the years to organizing hospital workers, and the first breakthroughs, in New York City hospitals, in 1958. From a start with lowest income hospital workers, the subsequent inclusion of technical and professional workers and linkages with the highly publicized hospital workers' strike in Charleston, South Carolina in 1969. The benefits of unionization and the broader social goals of the union are discussed."}
{"id": "PMID:1099015", "title": "Unionization, strikes, threatened strikes, and hospitals--the view from hospital management.", "content": "The history of union organizing efforts in the hospital field is discussed in this article, along with the factors judged necessary for successful union organizing. The role played by labor legislation in the unionization of hospital workers is shown, and the influences of the National Labor Relations Act, the Taft-Hartley amendments, and labor legislation at the local level are described. Management has largely resisted unionization because of the social nature of hospitals. Competitive market forces do not confront the not-for-profit hospitals, which are dependent upon third-party reimbursement. While strikes are an integral and essential part of collective bargaining in industry, they are, in fact, detrimental to hospitals because of these institutions' concern with human life. Despite laws and assurances from labor leaders that strikes will not occur, strikes have been used as a method for resolving disputes, through they are basically inconsistent with the economic characteristics and objectives of the hospital. The authors conclude that arbitration awards should be made by arbitrators appointed from outside of the local region of the hospital involved, ant that, because of the catastrophic effect of strikes upon patients as well as employees, arbitration awards should be required, should be binding upon both parties, and should be federally enforced.", "contents": "Unionization, strikes, threatened strikes, and hospitals--the view from hospital management. The history of union organizing efforts in the hospital field is discussed in this article, along with the factors judged necessary for successful union organizing. The role played by labor legislation in the unionization of hospital workers is shown, and the influences of the National Labor Relations Act, the Taft-Hartley amendments, and labor legislation at the local level are described. Management has largely resisted unionization because of the social nature of hospitals. Competitive market forces do not confront the not-for-profit hospitals, which are dependent upon third-party reimbursement. While strikes are an integral and essential part of collective bargaining in industry, they are, in fact, detrimental to hospitals because of these institutions' concern with human life. Despite laws and assurances from labor leaders that strikes will not occur, strikes have been used as a method for resolving disputes, through they are basically inconsistent with the economic characteristics and objectives of the hospital. The authors conclude that arbitration awards should be made by arbitrators appointed from outside of the local region of the hospital involved, ant that, because of the catastrophic effect of strikes upon patients as well as employees, arbitration awards should be required, should be binding upon both parties, and should be federally enforced."}
{"id": "PMID:1099020", "title": "The chemotherapy of infections due to nocardia.", "content": "Recent studies in vitro have revealed marked susceptibility of Nocardia asteroides to minocycline and to the synergistic combination of ampicillin plus erythromycin. Sulfonamides remain as the proven and well-used agents for this potentially fatal infection seen commonly in the compriomised host.", "contents": "The chemotherapy of infections due to nocardia. Recent studies in vitro have revealed marked susceptibility of Nocardia asteroides to minocycline and to the synergistic combination of ampicillin plus erythromycin. Sulfonamides remain as the proven and well-used agents for this potentially fatal infection seen commonly in the compriomised host."}
{"id": "PMID:1099021", "title": "Clomacran in the treatment of schizophrenic patients: a comparison of two assessment methods.", "content": "In an uncontrolled clinical trial clomacran, the chlorpromazine analogue of the acridane series, was found to be therapeutically effective in the treatment of newly admitted schizophrenic patients. There was essentially no difference in the sensitivity of the two assessment instruments, i.e. BPRS and PAF, in the detection of therapeutic changes. On the other hand, the PAF, a 123-item scale, was found to be more sensitive for the description of a schizophrenic patient population than the BPRS, an 18-item scale.", "contents": "Clomacran in the treatment of schizophrenic patients: a comparison of two assessment methods. In an uncontrolled clinical trial clomacran, the chlorpromazine analogue of the acridane series, was found to be therapeutically effective in the treatment of newly admitted schizophrenic patients. There was essentially no difference in the sensitivity of the two assessment instruments, i.e. BPRS and PAF, in the detection of therapeutic changes. On the other hand, the PAF, a 123-item scale, was found to be more sensitive for the description of a schizophrenic patient population than the BPRS, an 18-item scale."}
{"id": "PMID:1099022", "title": "The effect of methylphenidate on cardiovascular sensory differentiation on the hyperkinetic syndrome.", "content": "In the present study, the action of methylphenidate on mean heart rate was studied while hyperkinetic children were attending to monosensory, bisensory and trisensory stimuli presentations. Neither children receiving methylphenidate nor those receiving placebo exhibited a significant shift in tonic heart rate during relaxation periods. While attending to sensory stimuli, heart rates were higher for children receiving placebo than for those receiving methylphenidate in all experimental conditions for the high and moderate degrees of hyperkinetic behavior. A drug reversal effect was demonstrated in mildly hyperkinetic children.", "contents": "The effect of methylphenidate on cardiovascular sensory differentiation on the hyperkinetic syndrome. In the present study, the action of methylphenidate on mean heart rate was studied while hyperkinetic children were attending to monosensory, bisensory and trisensory stimuli presentations. Neither children receiving methylphenidate nor those receiving placebo exhibited a significant shift in tonic heart rate during relaxation periods. While attending to sensory stimuli, heart rates were higher for children receiving placebo than for those receiving methylphenidate in all experimental conditions for the high and moderate degrees of hyperkinetic behavior. A drug reversal effect was demonstrated in mildly hyperkinetic children."}
{"id": "PMID:1099017", "title": "Effect of quinacrine, chloroquine and primaquine on the multiplication of Mycobacterium leprae in mice.", "content": "Quinacrine administered in 100 mg per kg body weight to mice had a bacteriostatic activity on M. leprae in the mouse, chloroquine at 15 mg per kg and primaquine at 0.25 mg per kg were without activity. These findings could point to the presence of a functioning direct oxidative pathway of glucose catabolism in M. leprae.", "contents": "Effect of quinacrine, chloroquine and primaquine on the multiplication of Mycobacterium leprae in mice. Quinacrine administered in 100 mg per kg body weight to mice had a bacteriostatic activity on M. leprae in the mouse, chloroquine at 15 mg per kg and primaquine at 0.25 mg per kg were without activity. These findings could point to the presence of a functioning direct oxidative pathway of glucose catabolism in M. leprae."}
{"id": "PMID:1099018", "title": "Separation of Mycobacterium lepraemurium from the subcutaneous tissues of the rat.", "content": "In an effort to obtain cell suspensions free from blood cells which interfere in spectrophotometric studies, a method was devised by which relatively large quantities of whole cell suspensions of M. lepraemurium are obtained for experimental purposes. We have routinely employed this procedure which is quite reliable, technically simple, moderate in equipment requirements, and in a matter of five minutes it can be ascertained whether or not the preparations are free from contaminants which render the purified bacillary suspensions unsuitable for studies involving spectrophotometric technics. However, in this procedure, certain points are essential in order to obtain large quantities of purified bacillary suspensions. It is important to homogenize the lepromata for only a few seconds at brief intervals. Excessive homogenization may cause considerable damage to the bacilli and it may be difficult to separate the bacilli from the host tissues. It is also important to use transparent bottles and tubes during centrifugation as the material can be seen and thus each fraction can be separated easily. Very few bacilli are lost in the discarded material and we have repeatedly obtained 2.5 to 3 gm wet weight of M. lepraemurium from one leproma weighing 25 gm to 30 gm.", "contents": "Separation of Mycobacterium lepraemurium from the subcutaneous tissues of the rat. In an effort to obtain cell suspensions free from blood cells which interfere in spectrophotometric studies, a method was devised by which relatively large quantities of whole cell suspensions of M. lepraemurium are obtained for experimental purposes. We have routinely employed this procedure which is quite reliable, technically simple, moderate in equipment requirements, and in a matter of five minutes it can be ascertained whether or not the preparations are free from contaminants which render the purified bacillary suspensions unsuitable for studies involving spectrophotometric technics. However, in this procedure, certain points are essential in order to obtain large quantities of purified bacillary suspensions. It is important to homogenize the lepromata for only a few seconds at brief intervals. Excessive homogenization may cause considerable damage to the bacilli and it may be difficult to separate the bacilli from the host tissues. It is also important to use transparent bottles and tubes during centrifugation as the material can be seen and thus each fraction can be separated easily. Very few bacilli are lost in the discarded material and we have repeatedly obtained 2.5 to 3 gm wet weight of M. lepraemurium from one leproma weighing 25 gm to 30 gm."}
{"id": "PMID:1099016", "title": "Acid mucopolysaccharide metabolism in leprosy. 3. Hyaluronic acid mycobacterial growth enhancement, and growth suppression by saccharic acid and vitamin C as inhibitors of beta-glucuronidase.", "content": "A series of pilot studies are presented utilizing mouse and human infections with M. leprae and mouse infections with M. lepraemurium relating to the previously reported finding that hyaluronic acid seems to be a major nutrient substrate for these bacilli. The \"feeding\" of hyaluronic acid to the bacilli enhanced the growth of M. leprae in mouse abdominal walls and increased the Morphologic Index of M. lepraemurium infection. Saccharic acid, an inhibitor of beta-glucuronidase previously reported as present in these leprosy bacilli, caused marked regression of advanced M. lepraemurium infection, inhii. Ascorbic acid (vitamin C), also an inhibitor of beta-glucuronidase, given at a level of 1.5 gm/day for 4.5 months to one lepromatous patient without other treatment and for up to 24 months to four other lepromatous patients receiving DDS, was accompanied by lesion regression and changes in bacillary morphology similar to those seen in the inhibitor treated mice. If these observations are confirmed the possible use of beta-glucuronidase inhibitors as a useful adjunct to other leprosy therapy is raised as is also the likelihood of developing new therapies.", "contents": "Acid mucopolysaccharide metabolism in leprosy. 3. Hyaluronic acid mycobacterial growth enhancement, and growth suppression by saccharic acid and vitamin C as inhibitors of beta-glucuronidase. A series of pilot studies are presented utilizing mouse and human infections with M. leprae and mouse infections with M. lepraemurium relating to the previously reported finding that hyaluronic acid seems to be a major nutrient substrate for these bacilli. The \"feeding\" of hyaluronic acid to the bacilli enhanced the growth of M. leprae in mouse abdominal walls and increased the Morphologic Index of M. lepraemurium infection. Saccharic acid, an inhibitor of beta-glucuronidase previously reported as present in these leprosy bacilli, caused marked regression of advanced M. lepraemurium infection, inhii. Ascorbic acid (vitamin C), also an inhibitor of beta-glucuronidase, given at a level of 1.5 gm/day for 4.5 months to one lepromatous patient without other treatment and for up to 24 months to four other lepromatous patients receiving DDS, was accompanied by lesion regression and changes in bacillary morphology similar to those seen in the inhibitor treated mice. If these observations are confirmed the possible use of beta-glucuronidase inhibitors as a useful adjunct to other leprosy therapy is raised as is also the likelihood of developing new therapies."}
{"id": "PMID:1099023", "title": "Chlorpromazine-induced electrocardiogram abnormalities.", "content": "The aim of our clinical investigation was to evaluate the electrocardiographic abnormalities in patients receiving only chlorpromazine, and to assess the role of sex, age and length of illness in these abnormalities. 96 schizophrenic patients (48 male and 48 female patients) satisfying study criteria were selected for inclusion and changed from their old medication to a fixed dose of 125 mg chlorpromazine daily. This uniformization period lasted two weeks, during which the electrocardiograms were taken after an overnight fast. Five patients were excluded because their electrocardiographic changes revealed the possibility of an organic heart disease. The electrocardiograms were found to be normal for 65.9% of the patients and abnormal for 34.1%. 11% of patients were classified as demonstrating Grade I repolarization abnormalities, 14.3% of patients as Grade II, and 8.8% as Grade III. Chi square (X2) tests revealed no significant differences between sexes, age groups or lenghts of illness as regards ECG normality and grade of abnormality.", "contents": "Chlorpromazine-induced electrocardiogram abnormalities. The aim of our clinical investigation was to evaluate the electrocardiographic abnormalities in patients receiving only chlorpromazine, and to assess the role of sex, age and length of illness in these abnormalities. 96 schizophrenic patients (48 male and 48 female patients) satisfying study criteria were selected for inclusion and changed from their old medication to a fixed dose of 125 mg chlorpromazine daily. This uniformization period lasted two weeks, during which the electrocardiograms were taken after an overnight fast. Five patients were excluded because their electrocardiographic changes revealed the possibility of an organic heart disease. The electrocardiograms were found to be normal for 65.9% of the patients and abnormal for 34.1%. 11% of patients were classified as demonstrating Grade I repolarization abnormalities, 14.3% of patients as Grade II, and 8.8% as Grade III. Chi square (X2) tests revealed no significant differences between sexes, age groups or lenghts of illness as regards ECG normality and grade of abnormality."}
{"id": "PMID:1099024", "title": "Thiosemicarbazones and hydrazones of alpha-methylchalkone as potential chemotherapeutic agents.", "content": "The effectiveness of chalkones and derivatives as antibacterial and antifungal agents stimulated our interest in the possibility of coupling this type of compound with certain hydrazines and thiosemicarbazides to determine the potential chemotherapeutic activity of these combinations as anticancer and antimalarial agents. Accordingly, 18 hydrazine and thiosemicarbazide derivatives of alpha-methylchalkone (dypnone) have been synthesized for study as potential antitumor agents in animal tumor systems against Walker 256 carcinosarcoma (intramuscular) and leukemia L-1210 and for antimalarial activity against Plasmodium berghei in experimentally infected mice. Of the series of chalkone derivatives, significant inhibition in preliminary tests against the Walker 256 carcinosarcoma (intramuscular) rat tumor system was exhibited by alpha-methylchalkone-1,4-phthalazinediyldihydrazone and showed activity in the leukemia 1210 mouse tumor system. The guanylhydrazone of alpha-methylchalkone showed good inhibition with confirmed activity against Plasmodium berghei in experimentally infected mice.", "contents": "Thiosemicarbazones and hydrazones of alpha-methylchalkone as potential chemotherapeutic agents. The effectiveness of chalkones and derivatives as antibacterial and antifungal agents stimulated our interest in the possibility of coupling this type of compound with certain hydrazines and thiosemicarbazides to determine the potential chemotherapeutic activity of these combinations as anticancer and antimalarial agents. Accordingly, 18 hydrazine and thiosemicarbazide derivatives of alpha-methylchalkone (dypnone) have been synthesized for study as potential antitumor agents in animal tumor systems against Walker 256 carcinosarcoma (intramuscular) and leukemia L-1210 and for antimalarial activity against Plasmodium berghei in experimentally infected mice. Of the series of chalkone derivatives, significant inhibition in preliminary tests against the Walker 256 carcinosarcoma (intramuscular) rat tumor system was exhibited by alpha-methylchalkone-1,4-phthalazinediyldihydrazone and showed activity in the leukemia 1210 mouse tumor system. The guanylhydrazone of alpha-methylchalkone showed good inhibition with confirmed activity against Plasmodium berghei in experimentally infected mice."}
{"id": "PMID:1099019", "title": "A comparative evaluation of bacteriologic and morphologic indices of Mycobacterium leprae in skin, lymph node, bone marrow, nerve and muscle.", "content": "This study elicits the comparative evaluation of the Bacteriologic Index (BI) and Morphologic Index (MI) of the skin, lymph node, bone marrow, nerve and muscle tissues of 15 untreated leprosy patients. The findings of comparable or even higher BI's and MI's in lymph nodes than skin are of considerable interest, especially the presence of a significant percentage of viable bacilli during reaction states when MI of skin and nerve tissue was found to be negative. Evaluation of the BI's and MI's of lymph nodes has been suggested to be of utmost importance for follow-up studies of patients being treated with antileprosy drugs.", "contents": "A comparative evaluation of bacteriologic and morphologic indices of Mycobacterium leprae in skin, lymph node, bone marrow, nerve and muscle. This study elicits the comparative evaluation of the Bacteriologic Index (BI) and Morphologic Index (MI) of the skin, lymph node, bone marrow, nerve and muscle tissues of 15 untreated leprosy patients. The findings of comparable or even higher BI's and MI's in lymph nodes than skin are of considerable interest, especially the presence of a significant percentage of viable bacilli during reaction states when MI of skin and nerve tissue was found to be negative. Evaluation of the BI's and MI's of lymph nodes has been suggested to be of utmost importance for follow-up studies of patients being treated with antileprosy drugs."}
{"id": "PMID:1099025", "title": "Statistical problems in interpreting comparative bioavailability trials.", "content": "The basic purpose of a comparative bioavailability is to determine whether or not a new formulation of a drug is equivalent in its in vivo behavior to some standard formulation. Statistical analysis of the blood or urine concentrations of the drug plays a critical role in this determination, and two major problems are in deciding what to analyse and what statistical methods to use. It is proposed that the analysis usually employed, namely, hypothesis-testing, is largely irrelevant to the problem and should be replaced by an approach based on confidence intervals. Secondly, it is suggested that only those characteristics of the bood or urine-level sequence that have a meaningful relationship to the therapeutic use of the drug, should be analysed.", "contents": "Statistical problems in interpreting comparative bioavailability trials. The basic purpose of a comparative bioavailability is to determine whether or not a new formulation of a drug is equivalent in its in vivo behavior to some standard formulation. Statistical analysis of the blood or urine concentrations of the drug plays a critical role in this determination, and two major problems are in deciding what to analyse and what statistical methods to use. It is proposed that the analysis usually employed, namely, hypothesis-testing, is largely irrelevant to the problem and should be replaced by an approach based on confidence intervals. Secondly, it is suggested that only those characteristics of the bood or urine-level sequence that have a meaningful relationship to the therapeutic use of the drug, should be analysed."}
{"id": "PMID:1099026", "title": "Bioavailability studies and the clinical pharmacologist: correlation of drug distribution with clinical effects.", "content": "Bioavailability studies can be interpreted only when clinical information is available which correlates drug distribution with both efficacious and toxic clinical effects. The clinical pharmacologist should be instrumental in the development of safe and effective drug therapy by the systematic application of well designed clinical trials, the clear definition of therapeutically desirable clinical responses, and the development and application of improved techniques of measuring these effects.", "contents": "Bioavailability studies and the clinical pharmacologist: correlation of drug distribution with clinical effects. Bioavailability studies can be interpreted only when clinical information is available which correlates drug distribution with both efficacious and toxic clinical effects. The clinical pharmacologist should be instrumental in the development of safe and effective drug therapy by the systematic application of well designed clinical trials, the clear definition of therapeutically desirable clinical responses, and the development and application of improved techniques of measuring these effects."}
{"id": "PMID:1099027", "title": "Toxoplasmosis and the compromised host.", "content": "A high percentage of population shows antibodies to Toxoplasma gondii yet clinical manifestations of primary infestations are rare. Congenital and secondary forms (chorioretinitis) are serious medical conditions. An outburst in a compromised host is always serious and may be fatal. Therefore, patients subjected to immunosuppressive therapy or with pathologic immune suppression should be clinically and serologically screened and, when toxoplasmosis is diagnosed, treated for the entire period of immunosuppression.", "contents": "Toxoplasmosis and the compromised host. A high percentage of population shows antibodies to Toxoplasma gondii yet clinical manifestations of primary infestations are rare. Congenital and secondary forms (chorioretinitis) are serious medical conditions. An outburst in a compromised host is always serious and may be fatal. Therefore, patients subjected to immunosuppressive therapy or with pathologic immune suppression should be clinically and serologically screened and, when toxoplasmosis is diagnosed, treated for the entire period of immunosuppression."}
{"id": "PMID:1099036", "title": "Tinea versicolor hypopigmentation: histochemical and therapeutic studies.", "content": "One hundred and thirty-four patients with tinea versicolor were divided into 3 treatment groups: (1) 30% sodium thiosulfate application, (2) 30% sodium thiosulfate application plus ultraviolet light, and (3) 30% sodium thiosulfate application, ultraviolet light, and meladinine tablets (amoidin 0.01 g and amidin 0.005 g). Analysis of the 79 patients who completed the 6-week study showed no statistical difference. Therefore, topical treatment should be instituted as quickly as possible to prevent pigmentary changes. Ultraviolet light should be used only after the fungus has cleared in order not to darken the skin further.", "contents": "Tinea versicolor hypopigmentation: histochemical and therapeutic studies. One hundred and thirty-four patients with tinea versicolor were divided into 3 treatment groups: (1) 30% sodium thiosulfate application, (2) 30% sodium thiosulfate application plus ultraviolet light, and (3) 30% sodium thiosulfate application, ultraviolet light, and meladinine tablets (amoidin 0.01 g and amidin 0.005 g). Analysis of the 79 patients who completed the 6-week study showed no statistical difference. Therefore, topical treatment should be instituted as quickly as possible to prevent pigmentary changes. Ultraviolet light should be used only after the fungus has cleared in order not to darken the skin further."}
{"id": "PMID:1099031", "title": "Diagnostic significance of the immunofluorescent pattern in dermatitis herpetiformis.", "content": "Despite significant clinical, histologic, immunopathologic and other differences between typical cases of dermatitis herpetiformis (DH) and bullous pemphigoid (BP), the differential diagnosis may be difficult. Our direct immunofluorescent studies in 59 cases of DH and 134 cases of BP indicate the diagnostic value of immunopathologic studies.", "contents": "Diagnostic significance of the immunofluorescent pattern in dermatitis herpetiformis. Despite significant clinical, histologic, immunopathologic and other differences between typical cases of dermatitis herpetiformis (DH) and bullous pemphigoid (BP), the differential diagnosis may be difficult. Our direct immunofluorescent studies in 59 cases of DH and 134 cases of BP indicate the diagnostic value of immunopathologic studies."}
{"id": "PMID:1099032", "title": "Urea creams in the treatment of dry skin and hand dermatitis.", "content": "Two randomized, double-blind comparison studies were conducted to evaluate the efforts of 2 emollient dry skin creams containing urea in the management of dry skin in atopic patients. Cream with 10% urea was shown to be effective in the management of dry skin and hand dermatitis. The cream with a pH of 6 caused less burning.", "contents": "Urea creams in the treatment of dry skin and hand dermatitis. Two randomized, double-blind comparison studies were conducted to evaluate the efforts of 2 emollient dry skin creams containing urea in the management of dry skin in atopic patients. Cream with 10% urea was shown to be effective in the management of dry skin and hand dermatitis. The cream with a pH of 6 caused less burning."}
{"id": "PMID:1099041", "title": "Psychoanalysis as a theory of therapy.", "content": "The development of psychoanalytic theory is traced from its origins in Freud's early thinking in terms of his advancing clinical experience. While the influence of clinical experience on the modifications of theory has long been accepted, little attention has been paid to the broad spectrum effects of changing theoretical perspectives on the technique of clinical psychoanalysis. This development is traced through the emergence of ego psychology and the dominance of the structural theory to more current developments having to do with object relations and the theory narcissism. The residual theoretical ambiguities and clinical perplexities are illustrated in two clinical cases. The development of psychoanalytic theory and the correlative aspects of technique are seen as manifesting an organic developmental process which has led to an increasing sophistication of analytic intervention and a broadening capacity for dealing with increasingly difficult and less classical types of cases. The conclusion is drawn that therapeutic lestening is equivalently an informed listening, and it is theory that lends understanding and sensitivity to the hearing process. It is theory that is to be put in the service of listening, rather than listening to be put in the service of theory.", "contents": "Psychoanalysis as a theory of therapy. The development of psychoanalytic theory is traced from its origins in Freud's early thinking in terms of his advancing clinical experience. While the influence of clinical experience on the modifications of theory has long been accepted, little attention has been paid to the broad spectrum effects of changing theoretical perspectives on the technique of clinical psychoanalysis. This development is traced through the emergence of ego psychology and the dominance of the structural theory to more current developments having to do with object relations and the theory narcissism. The residual theoretical ambiguities and clinical perplexities are illustrated in two clinical cases. The development of psychoanalytic theory and the correlative aspects of technique are seen as manifesting an organic developmental process which has led to an increasing sophistication of analytic intervention and a broadening capacity for dealing with increasingly difficult and less classical types of cases. The conclusion is drawn that therapeutic lestening is equivalently an informed listening, and it is theory that lends understanding and sensitivity to the hearing process. It is theory that is to be put in the service of listening, rather than listening to be put in the service of theory."}
{"id": "PMID:1099045", "title": "Side effects of tricyclic antidepressand drugs with particular reference fo dothiepin.", "content": "Fifteen patients on dothiepin and 20 on other tricyclic compounds were compared for side effects by self-rating. The high incidence of complaints beforehand and overall reduction during 2 weeks treatment, correlating with clinical improvement, shows that comparison of side effects is unreliable unless pretreatment incidence is recorded.", "contents": "Side effects of tricyclic antidepressand drugs with particular reference fo dothiepin. Fifteen patients on dothiepin and 20 on other tricyclic compounds were compared for side effects by self-rating. The high incidence of complaints beforehand and overall reduction during 2 weeks treatment, correlating with clinical improvement, shows that comparison of side effects is unreliable unless pretreatment incidence is recorded."}
{"id": "PMID:1099046", "title": "The Hamilton rating scale. An assessment bases on a dothiepin (\"prothiaden\") versus imipramine (\"Tofranil\") clinical trial.", "content": "The Hamilton Rating Scale for Depression is in common use, particularly in durg trials. The authors compared this scale with a system of psychiatric assessment and recording designed by M. Sim. They found that there was closer agreement with the full assessment of the Sim method than with the single diagnostic area for depression. This suggests that the Hamilton Scale for depression is more general than specific in its application.", "contents": "The Hamilton rating scale. An assessment bases on a dothiepin (\"prothiaden\") versus imipramine (\"Tofranil\") clinical trial. The Hamilton Rating Scale for Depression is in common use, particularly in durg trials. The authors compared this scale with a system of psychiatric assessment and recording designed by M. Sim. They found that there was closer agreement with the full assessment of the Sim method than with the single diagnostic area for depression. This suggests that the Hamilton Scale for depression is more general than specific in its application."}
{"id": "PMID:1099047", "title": "Contingent naloxone (N-allylnoroxymorphone) treatment of the paroled narcotic addict.", "content": "This is a presentation of the results of pilot and controlled research on the effectiveness of the contingent (upon narcotic drug use) administration of 500-2,000 mg daily, of the narcotic antagonist, naloxone (N-allylnoroxymorphone), to paroled narcotic addicts enrolled in a urine monitoring program conducted in a metropolitan-based outpatient clinic. Criteria of effectiveness, which include clinic attendance, the extent of narcotic drug usage, and final disposition at the end of a 6-month treatment period, are viewed in relation to already established baseline results with  a sample of patients processed through the same clinic over a 5-year period prior to the introduction of naloxone treatment. Although results of the pilot study are encouraging, indicating longer patient involvement and less reinstitutionalization than baseline values, the results of the controlled evaluation reveal no benefit from contingently administered naloxone beyond placebo reactivity, which appears to be substantial in the contingent approach. The results are discussed in terms of given sample characteristics, and suggestions are offered regarding the development of new narcotic antagonist treatment approaches.", "contents": "Contingent naloxone (N-allylnoroxymorphone) treatment of the paroled narcotic addict. This is a presentation of the results of pilot and controlled research on the effectiveness of the contingent (upon narcotic drug use) administration of 500-2,000 mg daily, of the narcotic antagonist, naloxone (N-allylnoroxymorphone), to paroled narcotic addicts enrolled in a urine monitoring program conducted in a metropolitan-based outpatient clinic. Criteria of effectiveness, which include clinic attendance, the extent of narcotic drug usage, and final disposition at the end of a 6-month treatment period, are viewed in relation to already established baseline results with  a sample of patients processed through the same clinic over a 5-year period prior to the introduction of naloxone treatment. Although results of the pilot study are encouraging, indicating longer patient involvement and less reinstitutionalization than baseline values, the results of the controlled evaluation reveal no benefit from contingently administered naloxone beyond placebo reactivity, which appears to be substantial in the contingent approach. The results are discussed in terms of given sample characteristics, and suggestions are offered regarding the development of new narcotic antagonist treatment approaches."}
{"id": "PMID:1099048", "title": "Effects of control techniques on therapeutic outcome in a controlled clinical trial.", "content": "Undertaken in the context of a controlled drug trial involving newly admitted psychiatric hospital patients, this study compared the effectiveness of a \"Doctor's Choice\" (DC) method of administering psychotropic drugs versus an experimentally determined treatment regimen employing random assignment and double-bline procedures. The 32-day drug trial sought to determine the comparative effectiveness of thioridazine-placebo, thioridazine-chlordiazepoxide, and thioridazine-imipramine, with the daily dosage of openly administered thioridazine ranging from 100 to 900 mg and dosages for chlordiazepoxide and imipramine, administered in a double-blind fashion, fixed at daily dosages of 30 and 75 mg, respectively. DC medication, consisting of a choice (by a research physician) of any of the three experimental medications determined on the basis of judged clinical need, was added as a fourth treatment category for present purposes. Criteria of effectiveness included standarized psychiatric rating scales and global measures of imporvement completed by research team members and ward physicians. Outcome results for the DC group compared to those for a single control group made up of individuals matched with DC patients on the basis of drug assignment indicated an essentially similar clinical effectiveness under both DC and control treatment conditions. Generalization of the finding was limited by the fact that the main treatment effect, attributable to thioridazine, overshadowed the more subtle action of the ancillary drugs.", "contents": "Effects of control techniques on therapeutic outcome in a controlled clinical trial. Undertaken in the context of a controlled drug trial involving newly admitted psychiatric hospital patients, this study compared the effectiveness of a \"Doctor's Choice\" (DC) method of administering psychotropic drugs versus an experimentally determined treatment regimen employing random assignment and double-bline procedures. The 32-day drug trial sought to determine the comparative effectiveness of thioridazine-placebo, thioridazine-chlordiazepoxide, and thioridazine-imipramine, with the daily dosage of openly administered thioridazine ranging from 100 to 900 mg and dosages for chlordiazepoxide and imipramine, administered in a double-blind fashion, fixed at daily dosages of 30 and 75 mg, respectively. DC medication, consisting of a choice (by a research physician) of any of the three experimental medications determined on the basis of judged clinical need, was added as a fourth treatment category for present purposes. Criteria of effectiveness included standarized psychiatric rating scales and global measures of imporvement completed by research team members and ward physicians. Outcome results for the DC group compared to those for a single control group made up of individuals matched with DC patients on the basis of drug assignment indicated an essentially similar clinical effectiveness under both DC and control treatment conditions. Generalization of the finding was limited by the fact that the main treatment effect, attributable to thioridazine, overshadowed the more subtle action of the ancillary drugs."}
{"id": "PMID:1099056", "title": "[Severe light dermatosis following photo therapy in a newborn infant with congenital erythropoietic urophyria].", "content": "A newborn infant with hemolytic anemia and hepatosplenomegaly was treated by phototherapy for early jaundice. After 18 h, a dark brown pigmentation of the skin was noticed, leading to the assumption of a bronze baby syndrome. Indeed, the child was suffering from a severe disturbance of liver function. 4 days later, a severe bullous dermatosis with blody imbibition developed, covering all exposed parts of the body surface and reoccurring in many bursts over several weeks despite protection against light. A severe hemolytic anemia was constantly present. The baby died on the 50th day. The diagnosis of erythropoietic porphyria was suggested immediately after the onset of the bullous exanthema and proved by laboratory data as follows: uro- and coproporphyrin in the urine were extremely high, uroporphyrin being mainly of type-I isomer. In red cells, increased amounts of uro-, copro- and protoporphyrins were detected. Massive red fluorescence of erythroblasts (so-called porphyroblasts) in the bone marrow and in the blood could be observed. At autopsy, the liver showed multiple blood-forming areas and severe diffuse hemosiderosis, which is to be explained by a long existing, i.e. fetal hemolysis. Erythropoietic porphyria is such a rare disease that there is no reason to consider it as a general contraindication for phototherapy.", "contents": "[Severe light dermatosis following photo therapy in a newborn infant with congenital erythropoietic urophyria]. A newborn infant with hemolytic anemia and hepatosplenomegaly was treated by phototherapy for early jaundice. After 18 h, a dark brown pigmentation of the skin was noticed, leading to the assumption of a bronze baby syndrome. Indeed, the child was suffering from a severe disturbance of liver function. 4 days later, a severe bullous dermatosis with blody imbibition developed, covering all exposed parts of the body surface and reoccurring in many bursts over several weeks despite protection against light. A severe hemolytic anemia was constantly present. The baby died on the 50th day. The diagnosis of erythropoietic porphyria was suggested immediately after the onset of the bullous exanthema and proved by laboratory data as follows: uro- and coproporphyrin in the urine were extremely high, uroporphyrin being mainly of type-I isomer. In red cells, increased amounts of uro-, copro- and protoporphyrins were detected. Massive red fluorescence of erythroblasts (so-called porphyroblasts) in the bone marrow and in the blood could be observed. At autopsy, the liver showed multiple blood-forming areas and severe diffuse hemosiderosis, which is to be explained by a long existing, i.e. fetal hemolysis. Erythropoietic porphyria is such a rare disease that there is no reason to consider it as a general contraindication for phototherapy."}
{"id": "PMID:1099057", "title": "Catalytically inactive sucrase antigen of rabbit small intestine: the enzyme precursor.", "content": "By immunofluorescence microscopy using a specific antibody to the active rabbit sucrase-isomaltase complex, a catalytically inactive sucrase antigen was discovered on the small intestinal mucosa of young rabbits still lacking sucrase activity. In adult rabbits, the same antigen was demonstrated on enterocytes of mucosal crypts devoid of sucrase. Catalytically inactive antigen was isolated by means of immobilized antibody to active sucrase, and it was compared with the active sucrase-isomaltase complex. From structural similarities between the 2 proteins and from the fact that active sucrase succeeds the inactive antigen in both the maturing and the mature rabbit, it is concluded that the inactive antigen is the enzyme precursor. In some patients with hereditary sucrase-isomaltase deficiency, abnormal persistence of sucrase precurosr due to a faulty activation mechanism may be the underlying defect.", "contents": "Catalytically inactive sucrase antigen of rabbit small intestine: the enzyme precursor. By immunofluorescence microscopy using a specific antibody to the active rabbit sucrase-isomaltase complex, a catalytically inactive sucrase antigen was discovered on the small intestinal mucosa of young rabbits still lacking sucrase activity. In adult rabbits, the same antigen was demonstrated on enterocytes of mucosal crypts devoid of sucrase. Catalytically inactive antigen was isolated by means of immobilized antibody to active sucrase, and it was compared with the active sucrase-isomaltase complex. From structural similarities between the 2 proteins and from the fact that active sucrase succeeds the inactive antigen in both the maturing and the mature rabbit, it is concluded that the inactive antigen is the enzyme precursor. In some patients with hereditary sucrase-isomaltase deficiency, abnormal persistence of sucrase precurosr due to a faulty activation mechanism may be the underlying defect."}
{"id": "PMID:1099060", "title": "Streptomycylamines: difference in activity and mode of action between short-chain and long-chain derivatives.", "content": "A homologue series of aliphatic streptomycylamines (SM-amines) have been prepared and tested in vitro (binding to 70S ribosomes) and in vivo (MIC).  The short-chain SM-amines act as streptomycin (SM) but are less active than SM.  They are inactive towards a SM-resistant Escherichia coli, our strain 042.  The long-chain SM-amines are active both towards our sensitive and resistant E. coli, our strains 079 and 042.  Their activities are not pH-dependent in contrast to that of SM.  However, the higher homologues of the aliphatic amines (C10 Congruent TO C10) are considerably active per se although two to four times less than the corresponding SM-amines.  Further, the amines do not compete with the SM-amine for the binding to the ribosomal particles.  The binding affinities of these long-chain SM-amines to ribosomes are considerably smaller than that of SM.  The binding is however specific as a typical isotope dilution curve can be obtained.  We conclude that the long-chain SM-amines have a mode of action different from that of SM.", "contents": "Streptomycylamines: difference in activity and mode of action between short-chain and long-chain derivatives. A homologue series of aliphatic streptomycylamines (SM-amines) have been prepared and tested in vitro (binding to 70S ribosomes) and in vivo (MIC).  The short-chain SM-amines act as streptomycin (SM) but are less active than SM.  They are inactive towards a SM-resistant Escherichia coli, our strain 042.  The long-chain SM-amines are active both towards our sensitive and resistant E. coli, our strains 079 and 042.  Their activities are not pH-dependent in contrast to that of SM.  However, the higher homologues of the aliphatic amines (C10 Congruent TO C10) are considerably active per se although two to four times less than the corresponding SM-amines.  Further, the amines do not compete with the SM-amine for the binding to the ribosomal particles.  The binding affinities of these long-chain SM-amines to ribosomes are considerably smaller than that of SM.  The binding is however specific as a typical isotope dilution curve can be obtained.  We conclude that the long-chain SM-amines have a mode of action different from that of SM."}
{"id": "PMID:1099065", "title": "Determination of ammonia inhibition in fabric impregnated with antimicrobials.", "content": "A new method is described for determining the presence or absence of ammonia produced by urealytic microorganisms, using fabric which has been treated with an antimicrobial. Circular disks of fabric treated with various concentrations of an antimicrobial are placed in the bottoms of snap-on lid petri dishes. Nutrient medium containing urea, an indicator, and a dilution of an overnight culture of Proteus mirabilis is applied to the fabric disks. The lids are snapped on and the dishes are incubated at 37 degrees C. By examination of the fabric disks at regular intervals of time for a color change from white to red, one may determine whether the concentration of antimicrobial in the fabric is sufficient to inhibit the production of ammonia by a urealytic microorganism.", "contents": "Determination of ammonia inhibition in fabric impregnated with antimicrobials. A new method is described for determining the presence or absence of ammonia produced by urealytic microorganisms, using fabric which has been treated with an antimicrobial. Circular disks of fabric treated with various concentrations of an antimicrobial are placed in the bottoms of snap-on lid petri dishes. Nutrient medium containing urea, an indicator, and a dilution of an overnight culture of Proteus mirabilis is applied to the fabric disks. The lids are snapped on and the dishes are incubated at 37 degrees C. By examination of the fabric disks at regular intervals of time for a color change from white to red, one may determine whether the concentration of antimicrobial in the fabric is sufficient to inhibit the production of ammonia by a urealytic microorganism."}
{"id": "PMID:1099066", "title": "Computerized Electroencephalogram. A model of understanding the brain function in childhood psychosis and its treatment.", "content": "Computer analysis of the electroencephalogram (CEEG) in psychotic children before and after pharmacotherapy, normal children of schizophrenic mothers, and matched normal children of normal parents indicated significant intergroup differences. The psychotic children had more slow, as well as very fast, EEG waves. With drug therapy the EEG showed a partial \"normalization,\" as fast EEG activity decreased. The EEG and auditory evoked potential of children of schizophrenic mothers were strikingly similar to those of psychotic children and schizophrenic adults, with significant decreases of the average EEG amplitude and the evoked potential latencies. Psychotic children were distinctly differentiated from the normal children by discriminant function analysis of the EEG and EP. Quantitative analysis of brain functions in the mentally ill can help determine the neurophysiological correlates of behavior, a more scientific diagnostic classification, prognosis, and selection of therapy.", "contents": "Computerized Electroencephalogram. A model of understanding the brain function in childhood psychosis and its treatment. Computer analysis of the electroencephalogram (CEEG) in psychotic children before and after pharmacotherapy, normal children of schizophrenic mothers, and matched normal children of normal parents indicated significant intergroup differences. The psychotic children had more slow, as well as very fast, EEG waves. With drug therapy the EEG showed a partial \"normalization,\" as fast EEG activity decreased. The EEG and auditory evoked potential of children of schizophrenic mothers were strikingly similar to those of psychotic children and schizophrenic adults, with significant decreases of the average EEG amplitude and the evoked potential latencies. Psychotic children were distinctly differentiated from the normal children by discriminant function analysis of the EEG and EP. Quantitative analysis of brain functions in the mentally ill can help determine the neurophysiological correlates of behavior, a more scientific diagnostic classification, prognosis, and selection of therapy."}
{"id": "PMID:1099067", "title": "Methionine-and S-adenosyl methionine-mediated repression in a methionyl-transfer ribonucleic-acid synthetase mutant of Saccharomyces cerevisiae.", "content": "A Saccharomyces cerevisiae mutant strain unable to grow at 38 C and bearing a modified methionyl-transfer ribonucleic acid (tRNA) synthetase has been studied. It has been shown that, in this mutant, the percentage of tRNAmet charged in vivo paralleled the degree of repressibility of methionine biosynthetic enzymes by exogenous methionine. On the contrary, the repression mediated by exogenous S-adenosylmethionine does not correlate with complete acylation of tRNAmet. Althought McLaughlin and Hartwell reported previously that the thermosensitivity and the defect in the methionyl-tRNA synthetase were due to the same genetic lesion (1969), no diffenence could be found in the methionyl-tRNA synthetase activity or in the pattern of repressibility of methionine biosynthetic pathway after growth at the premissive and at a semipermissive temperature. It appears that the mutant also exhibits some other modified characters that render unlikely the existence of only one genetic lesion in this strain. A genetic study of this mutant was undertaken which led to the conclusion that the thermosensitivity and the other defects are not related to the methionyl-tRNA synthetase modification. It was shown that the modified repressibility of methionine biosynthetic enzymes by methionine and the lack of acylation of tRNAmet in vivo follow the methionyl-tRNA synthetase modification. These results are in favor of the idea that methionyl-tRNAmet, more likely than methionine, is implicated in the regulation of the biosynthesis of methionine.", "contents": "Methionine-and S-adenosyl methionine-mediated repression in a methionyl-transfer ribonucleic-acid synthetase mutant of Saccharomyces cerevisiae. A Saccharomyces cerevisiae mutant strain unable to grow at 38 C and bearing a modified methionyl-transfer ribonucleic acid (tRNA) synthetase has been studied. It has been shown that, in this mutant, the percentage of tRNAmet charged in vivo paralleled the degree of repressibility of methionine biosynthetic enzymes by exogenous methionine. On the contrary, the repression mediated by exogenous S-adenosylmethionine does not correlate with complete acylation of tRNAmet. Althought McLaughlin and Hartwell reported previously that the thermosensitivity and the defect in the methionyl-tRNA synthetase were due to the same genetic lesion (1969), no diffenence could be found in the methionyl-tRNA synthetase activity or in the pattern of repressibility of methionine biosynthetic pathway after growth at the premissive and at a semipermissive temperature. It appears that the mutant also exhibits some other modified characters that render unlikely the existence of only one genetic lesion in this strain. A genetic study of this mutant was undertaken which led to the conclusion that the thermosensitivity and the other defects are not related to the methionyl-tRNA synthetase modification. It was shown that the modified repressibility of methionine biosynthetic enzymes by methionine and the lack of acylation of tRNAmet in vivo follow the methionyl-tRNA synthetase modification. These results are in favor of the idea that methionyl-tRNAmet, more likely than methionine, is implicated in the regulation of the biosynthesis of methionine."}
{"id": "PMID:1099068", "title": "Derepression in Saccharomyces cerevisiae can be dissociated from cellular proliferation and deoxyribonucleic acid synthesis.", "content": "A method has been developed that permits precise control of release from catabolite repression in Saccharomyces cerevisiae. It consists of transferring cells growing exponentially on 5% glucose to derepression medium at high cell density. Derepression then proceeds with reproducible kinetics and is complete within 6 to 7.5 h for various intra- and extramitochondrial markers, in the absence of any substantial increase in cellular dry weight or protein. Nuclear (and mitochondrial) deoxyribonucleic acid synthesis can be interrupted in certain thermosensitive (cdc) mutants at the nonpermissive temperature; a shift to this temperature before the onset of derepression has no effect on its outcome.", "contents": "Derepression in Saccharomyces cerevisiae can be dissociated from cellular proliferation and deoxyribonucleic acid synthesis. A method has been developed that permits precise control of release from catabolite repression in Saccharomyces cerevisiae. It consists of transferring cells growing exponentially on 5% glucose to derepression medium at high cell density. Derepression then proceeds with reproducible kinetics and is complete within 6 to 7.5 h for various intra- and extramitochondrial markers, in the absence of any substantial increase in cellular dry weight or protein. Nuclear (and mitochondrial) deoxyribonucleic acid synthesis can be interrupted in certain thermosensitive (cdc) mutants at the nonpermissive temperature; a shift to this temperature before the onset of derepression has no effect on its outcome."}
{"id": "PMID:1099069", "title": "Transition of R factor NR1 in Proteus mirabilis: molecular structure and replication of NR1 deoxyribonucleic acid.", "content": "The structure of R factor NR1 DNA in Proteus mirabilis has been studied by using the techniques of CsCl density gradient centrifugation, sedimentation in neutral and alkaline sucrose gradients, and electron microscopy. It has been shown that the nontransitioned form of NR1 DNA isolated from P. mirabilis cultured in drug-free medium is a37-mum circular deoxyribonucleic acid (DNA) with a density of 1.712 g/ml in a neutral CsCl gradient. This circular molecule is a composite structure consisting of a 29-mum resistance transfer factor containing the tetracycline-resistance genes (RTF-TC) and an 8-mum r-determinants component conferring resistance to chloramphenicol (CM), streptomycin/spectinomycin, and the sulfonamides. There are one to two copies of NR1 per chromosome equivalent of DNA in exponential-phase cells cultured in Penassay broth. After growth of PM15/NR1 in medium containing 100 mug of CM per ml, the density of the NR1 DNA increased from 1.712 g/ml to approximately 1.718 g/ml and the proportion of NR1 DNA relative to the chromosome is amplified about 10-fold. The changes in R factor DNA structure which accompany this phenomenon (termed the transition) have been studied. DNA density profiles of the transitioned NR1 DNA consist of a 1.718 g/ml band which is skewed toward the less dense side. The transitioned NR1 DNA consists of molecules containing the RTF-TC element attached to multiple copies of r-determinants DNA (poly-r-determinant R factors) and multimeric and monomeric autonomous r-determinants structures. Poly-r-determinant R factors have a density intermediate between the basic composite structure (1.712 g/ml) and r-determinants DNA (1.718 g/ml). These species presumably account for the skewing of the 1.718-g/ml DNA band toward the less dense side. When transitioned cells are subsequently cultured in drug-free medium, poly-r-determinant R factors and autonomous poly-r-determinants undergo dissociation to form smaller structures containing fewer copies of r-determinants. This process continues until, after prolonged growth in drug-free medium the NR1 DNA returns to the nontransitioned state which consists of an RTF-TC and a single copy of r-determinants.", "contents": "Transition of R factor NR1 in Proteus mirabilis: molecular structure and replication of NR1 deoxyribonucleic acid. The structure of R factor NR1 DNA in Proteus mirabilis has been studied by using the techniques of CsCl density gradient centrifugation, sedimentation in neutral and alkaline sucrose gradients, and electron microscopy. It has been shown that the nontransitioned form of NR1 DNA isolated from P. mirabilis cultured in drug-free medium is a37-mum circular deoxyribonucleic acid (DNA) with a density of 1.712 g/ml in a neutral CsCl gradient. This circular molecule is a composite structure consisting of a 29-mum resistance transfer factor containing the tetracycline-resistance genes (RTF-TC) and an 8-mum r-determinants component conferring resistance to chloramphenicol (CM), streptomycin/spectinomycin, and the sulfonamides. There are one to two copies of NR1 per chromosome equivalent of DNA in exponential-phase cells cultured in Penassay broth. After growth of PM15/NR1 in medium containing 100 mug of CM per ml, the density of the NR1 DNA increased from 1.712 g/ml to approximately 1.718 g/ml and the proportion of NR1 DNA relative to the chromosome is amplified about 10-fold. The changes in R factor DNA structure which accompany this phenomenon (termed the transition) have been studied. DNA density profiles of the transitioned NR1 DNA consist of a 1.718 g/ml band which is skewed toward the less dense side. The transitioned NR1 DNA consists of molecules containing the RTF-TC element attached to multiple copies of r-determinants DNA (poly-r-determinant R factors) and multimeric and monomeric autonomous r-determinants structures. Poly-r-determinant R factors have a density intermediate between the basic composite structure (1.712 g/ml) and r-determinants DNA (1.718 g/ml). These species presumably account for the skewing of the 1.718-g/ml DNA band toward the less dense side. When transitioned cells are subsequently cultured in drug-free medium, poly-r-determinant R factors and autonomous poly-r-determinants undergo dissociation to form smaller structures containing fewer copies of r-determinants. This process continues until, after prolonged growth in drug-free medium the NR1 DNA returns to the nontransitioned state which consists of an RTF-TC and a single copy of r-determinants."}
{"id": "PMID:1099070", "title": "Denaturation mapping of R factor deoxyribonucleic acid.", "content": "The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants region of the composite R factor NR1 DNA which has a contour length of 37 mum and a density of 1.712 g/ml. The r-determinants region was a relatively undenatured 8.5-mum segment of the molecule when the deoxyribonucleic acid was partially denatured at pH 10.7. An RTF-TC genetic segregant of NR1 which had lost the r-determinants component had a contour length of 28.7 mum and a density of 1.710 g/ml. Characterization of an RTF-TC using partial denaturation mapping at pH 10.7 confirmed that the relatively undenatured 8.5-mum r-determinants segment of the composite R factor had been deleted. Circular, transitioned NR1 DNA molecules (1.716 to 1.718 g/ml), whose contour lengths were consistent with an RTF-TC plus an integral number of tandem copies of r-determinants, were also characterized by denaturation mapping. The relatively undenatured region in these molecules had a length equal to an integral number of copies of r-determinants and was located at the same site in the partially denatured RTF-TC as the single copy of r-determinants in the 37-mum composite NR1. This indicates that there is a unique integration site for r-determinants in the RTF-TC component. The R factor UCR122, a TC deletion mutant of NR1, was also characterized by denaturation mapping. The translocation of the TC resistance gene(s) on the denaturation map permitted the alignment of the denaturation map with the heteroduplex map of Sharp et al. (u073). Linear and circular monomeric and presumed multimeric r-determinants DNA molecules (p = 1.718 g/ml) were partially denatured at a higher pH (11.10). The r-determinants multimers showed a repeating 8.3-mum (monomeric) partial denaturation pattern indicating a head-to-tail arrangement of monomers in these poly-r-determinant molecules.", "contents": "Denaturation mapping of R factor deoxyribonucleic acid. The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants region of the composite R factor NR1 DNA which has a contour length of 37 mum and a density of 1.712 g/ml. The r-determinants region was a relatively undenatured 8.5-mum segment of the molecule when the deoxyribonucleic acid was partially denatured at pH 10.7. An RTF-TC genetic segregant of NR1 which had lost the r-determinants component had a contour length of 28.7 mum and a density of 1.710 g/ml. Characterization of an RTF-TC using partial denaturation mapping at pH 10.7 confirmed that the relatively undenatured 8.5-mum r-determinants segment of the composite R factor had been deleted. Circular, transitioned NR1 DNA molecules (1.716 to 1.718 g/ml), whose contour lengths were consistent with an RTF-TC plus an integral number of tandem copies of r-determinants, were also characterized by denaturation mapping. The relatively undenatured region in these molecules had a length equal to an integral number of copies of r-determinants and was located at the same site in the partially denatured RTF-TC as the single copy of r-determinants in the 37-mum composite NR1. This indicates that there is a unique integration site for r-determinants in the RTF-TC component. The R factor UCR122, a TC deletion mutant of NR1, was also characterized by denaturation mapping. The translocation of the TC resistance gene(s) on the denaturation map permitted the alignment of the denaturation map with the heteroduplex map of Sharp et al. (u073). Linear and circular monomeric and presumed multimeric r-determinants DNA molecules (p = 1.718 g/ml) were partially denatured at a higher pH (11.10). The r-determinants multimers showed a repeating 8.3-mum (monomeric) partial denaturation pattern indicating a head-to-tail arrangement of monomers in these poly-r-determinant molecules."}
{"id": "PMID:1099071", "title": "Isolation of specialized transducing bacteriophage lambda carrying genes of the L-arabinose operon of Escherichia coli B/r.", "content": "A heat-inducible lysis-defective phage lambda (lambdacI857S7) has been integrated at multiple sites within the L-arabinose region (araCOIBAD) of a strain of Escherichia coli K-12 deleted for the normal lambda attachment site (lambdaattdelta). The lambda phage has become integrated with opposite orientations at two different loci within the aratb gene and with the \"normal\" orientation (clockwise N-RA-J) at a single site in the araC gene. The burst size, spontaneous-curing frequencies, and number of prophage harbored by each of the ara secondary-site lysogens have been determined. From these secondary-site lysogens it has been possible to generate plaque-forming ara-transducing phage (lambdapara) and defective ara-transducing phage (lambdadara), as well as defective leucine-transducing particles (lambdadleu). The construction and characterization of these lambdaara-transducing phage and their derivatives which carry genetically defined portions of the L-arabinose region are presented.", "contents": "Isolation of specialized transducing bacteriophage lambda carrying genes of the L-arabinose operon of Escherichia coli B/r. A heat-inducible lysis-defective phage lambda (lambdacI857S7) has been integrated at multiple sites within the L-arabinose region (araCOIBAD) of a strain of Escherichia coli K-12 deleted for the normal lambda attachment site (lambdaattdelta). The lambda phage has become integrated with opposite orientations at two different loci within the aratb gene and with the \"normal\" orientation (clockwise N-RA-J) at a single site in the araC gene. The burst size, spontaneous-curing frequencies, and number of prophage harbored by each of the ara secondary-site lysogens have been determined. From these secondary-site lysogens it has been possible to generate plaque-forming ara-transducing phage (lambdapara) and defective ara-transducing phage (lambdadara), as well as defective leucine-transducing particles (lambdadleu). The construction and characterization of these lambdaara-transducing phage and their derivatives which carry genetically defined portions of the L-arabinose region are presented."}
{"id": "PMID:1099072", "title": "Role of pyrimidine dimer excision in loss of potential streptomycin resistance mutations of ultraviolet-irradiated Escherichia coli on phosphate-buffered agar.", "content": "The frequency of ultraviolet (UV)-induced mutations to streptomycin resistance dropped rapidly when starved Escherichia coli strains WP-2 B/r and B/r T- were incubated on phosphate-buffered agar (PBA), but was reduced only slightly in a WP-2 hcr- mutant. During postirradiation, incubation viability remained approximately constant. Cells given an optimal recovery treatment with photo-reactivating light showed no further recovery if subsequently incubated on PBA. At least 70% of the mutations induced to streptomycin resistance by UV could be repaired. The loss of potential streptomycin-resistant mutants was markedly reduced in strain B/r T- when 5 mug of acriflavin or 700 mug of caffeine per ml was added to PBA. The excision of UV-induced thymine-containing dimers from E. coli tb/r T- was investigated. Dimer excision progressed more slowly when the cells were incubated on PBA containing acriflavin or caffeine. There was no congruity between the kinetics of dimer excision and the kinetics of mutant loss. Our results indicate that removal of potential streptomycin-resistant mutants is considerably faster than the excision of pyrimidine dimers.", "contents": "Role of pyrimidine dimer excision in loss of potential streptomycin resistance mutations of ultraviolet-irradiated Escherichia coli on phosphate-buffered agar. The frequency of ultraviolet (UV)-induced mutations to streptomycin resistance dropped rapidly when starved Escherichia coli strains WP-2 B/r and B/r T- were incubated on phosphate-buffered agar (PBA), but was reduced only slightly in a WP-2 hcr- mutant. During postirradiation, incubation viability remained approximately constant. Cells given an optimal recovery treatment with photo-reactivating light showed no further recovery if subsequently incubated on PBA. At least 70% of the mutations induced to streptomycin resistance by UV could be repaired. The loss of potential streptomycin-resistant mutants was markedly reduced in strain B/r T- when 5 mug of acriflavin or 700 mug of caffeine per ml was added to PBA. The excision of UV-induced thymine-containing dimers from E. coli tb/r T- was investigated. Dimer excision progressed more slowly when the cells were incubated on PBA containing acriflavin or caffeine. There was no congruity between the kinetics of dimer excision and the kinetics of mutant loss. Our results indicate that removal of potential streptomycin-resistant mutants is considerably faster than the excision of pyrimidine dimers."}
{"id": "PMID:1099073", "title": "N-terminal amino acid sequences of D-serine deaminases of wild-type and operator-constitutive strains of Escherichia coli K-12.", "content": "The N-terminal amino acid sequences of the D-serine deaminases from strains of Escherichia coli K-12 that harbor wild-type and high-level constitutive catabolite-insensitive operator-initiator regions are identical: Met-Ser-GluNH2-Ser-Gly-Arg-His-Cys. This result indicates that the operator-initiator region is probably distinct from the D-serine deaminase structural gene.", "contents": "N-terminal amino acid sequences of D-serine deaminases of wild-type and operator-constitutive strains of Escherichia coli K-12. The N-terminal amino acid sequences of the D-serine deaminases from strains of Escherichia coli K-12 that harbor wild-type and high-level constitutive catabolite-insensitive operator-initiator regions are identical: Met-Ser-GluNH2-Ser-Gly-Arg-His-Cys. This result indicates that the operator-initiator region is probably distinct from the D-serine deaminase structural gene."}
{"id": "PMID:1099074", "title": "Structural and biochemical alterations of Nocardia asteroides cell walls during its growth cycle.", "content": "Analysis of Nocardia asteroides 14759 cell walls were done to determine the chemical and structural composition during the growth cycle. It was found that the ultrastructural profiles of the cell wall become altered as the cultures aged. Chemical analysis revealed corresponding shifts in cell wall components as the culture went from lag to logarithmic to stationary phases of growth. The peptidoglycan from lag-phase cells (5 h) represented 15% of the total cell wall weight, and the percentage of peptidoglycan progressively increased so that, in 1-week stationary-phase cells, it represented approximately 40% of the total wall weight. In lag-phase cells it was found that 36% of the cell wall weight was lipid in nature, whereas stationary-phase cells had only 7% lipid in their wall. The overall sugar composition of the walls remained relatively constant at about 28 to 31% however, the arabinose to galactose ratio changed from approximately 1:1 in lag-phase to 2:1 in stationary-phase cells. Gas-liquid chromatography demonstrated that the fatty acids making up the cell wall lipids changed relative to one another as the cells aged. Based upon the removal of lipids by ethanol-ether, chloroform, and alkaline methanol extraction, it was shown that the classes of loosely and firmly associated lipids changed as the cells aged. Further, it was found that a carotenoid-like pigment associated to a C22 fatty acid increased in the cell wall as the culture stopped growing. Peptidolipid or lipoprotein was found to make up a significant part of the cell wall. This component increased in amount and varied in amino acid content as the culture aged. Analysis of the totally extracted basal layer of the cell wall (peptidoglycan plus arabinogalactan) showed that it too changed as the cells grew and fragmented. The data presented established that the cell wall of N. asteroides was structurally and chemically complex and that a progression of chemical and physical processes occurred within the wall as the cells developed through their growth cycle.", "contents": "Structural and biochemical alterations of Nocardia asteroides cell walls during its growth cycle. Analysis of Nocardia asteroides 14759 cell walls were done to determine the chemical and structural composition during the growth cycle. It was found that the ultrastructural profiles of the cell wall become altered as the cultures aged. Chemical analysis revealed corresponding shifts in cell wall components as the culture went from lag to logarithmic to stationary phases of growth. The peptidoglycan from lag-phase cells (5 h) represented 15% of the total cell wall weight, and the percentage of peptidoglycan progressively increased so that, in 1-week stationary-phase cells, it represented approximately 40% of the total wall weight. In lag-phase cells it was found that 36% of the cell wall weight was lipid in nature, whereas stationary-phase cells had only 7% lipid in their wall. The overall sugar composition of the walls remained relatively constant at about 28 to 31% however, the arabinose to galactose ratio changed from approximately 1:1 in lag-phase to 2:1 in stationary-phase cells. Gas-liquid chromatography demonstrated that the fatty acids making up the cell wall lipids changed relative to one another as the cells aged. Based upon the removal of lipids by ethanol-ether, chloroform, and alkaline methanol extraction, it was shown that the classes of loosely and firmly associated lipids changed as the cells aged. Further, it was found that a carotenoid-like pigment associated to a C22 fatty acid increased in the cell wall as the culture stopped growing. Peptidolipid or lipoprotein was found to make up a significant part of the cell wall. This component increased in amount and varied in amino acid content as the culture aged. Analysis of the totally extracted basal layer of the cell wall (peptidoglycan plus arabinogalactan) showed that it too changed as the cells grew and fragmented. The data presented established that the cell wall of N. asteroides was structurally and chemically complex and that a progression of chemical and physical processes occurred within the wall as the cells developed through their growth cycle."}
{"id": "PMID:1099075", "title": "Deletions fusing the hisG and hisD genes in Salmonella typhimurium.", "content": "Frameshift mutation hisD497 occurs in the operator-proximal portion of the Salmonella typhimurium gene coding for the dimeric protein, L-histidinol dehydrogenase (HDH). Rare revertants of hisD497 are deletions fusing the hisD gene to the adjacent preceding structural gene, hisG (adenosine 5'-triphosphate-PR transferase). HDH purified from one revertant, hisGD4908, contains subunits of approximately normal molecular weight but with no clearly demonstrable unique amino-terminal sequence. We propose that a combined inactive G-D polypeptide is synthesized and then cleaved at a number of closely juxtaposed sites by endoproteolytic activity. At least some of the resulting fragments then participate in formation of active HDH dimers.", "contents": "Deletions fusing the hisG and hisD genes in Salmonella typhimurium. Frameshift mutation hisD497 occurs in the operator-proximal portion of the Salmonella typhimurium gene coding for the dimeric protein, L-histidinol dehydrogenase (HDH). Rare revertants of hisD497 are deletions fusing the hisD gene to the adjacent preceding structural gene, hisG (adenosine 5'-triphosphate-PR transferase). HDH purified from one revertant, hisGD4908, contains subunits of approximately normal molecular weight but with no clearly demonstrable unique amino-terminal sequence. We propose that a combined inactive G-D polypeptide is synthesized and then cleaved at a number of closely juxtaposed sites by endoproteolytic activity. At least some of the resulting fragments then participate in formation of active HDH dimers."}
{"id": "PMID:1099076", "title": "Isolation of a hybrid F' factor-carrying Escherichia coli lactose region and Salmonella typhimurium histidine region, F42-400 (F' ts114 lac+, his+): its partial characterization and behavior in Salmonella typhimurium.", "content": "Episome F' ts114 lac+ (F42-114) was transferred into Salmonella typhimurium carrying an F'his+ (FS400) episome, and fused episome F' ts114 lac+, his+ (F42-400) was obtained. Episome F42-400 could be transferred to S. typhimurium, Escherichia coli and Klebsiella pneumoniae. Identification of the episome was based on: (i) temperature sensitivity of the Lac+ and His+ phenotypes; (ii) the fact that F- segregants, obtained after temperature curing or acridine orange curing, were simultaneously Lac- and His-; and (iii) linkage of lac+ with his+ in episomal transfers to E. coli and S. typhimurium. The frequency of episome transfer was influenced by the genotype of the donor. Plasmid LT2, prevalent in S. typhimurium LT2 strains, was suggested to be responsible for the low fertility of S. typhimurium donors. Episome F42-400 was capable of chromosome mobilization, and the extent of chromosome mobilization was not influenced by the presence or absence of the histidine region on the donor chromosome. Growth in a defined medium with acridine orange was able to cure F42-400. The frequency of curing was increased (the frequency of His+ cells was 0.0001%) if the cells were grown at 40 C in the presence of acridine orange. Selection for temperature-resistant Lac+, His+ derivatives in a strain without histidine deletion yielded Hfr strains. However, similar and stronger selections in strains without the chromosomal histidine region failed to yield Hfr strains. Our inability to obtain Hfr's in strains without the chromosomal histidine region was explained by assuming that the episome F42-400 has lost the F sites involved in integration into the S. typhimurium chromosome.", "contents": "Isolation of a hybrid F' factor-carrying Escherichia coli lactose region and Salmonella typhimurium histidine region, F42-400 (F' ts114 lac+, his+): its partial characterization and behavior in Salmonella typhimurium. Episome F' ts114 lac+ (F42-114) was transferred into Salmonella typhimurium carrying an F'his+ (FS400) episome, and fused episome F' ts114 lac+, his+ (F42-400) was obtained. Episome F42-400 could be transferred to S. typhimurium, Escherichia coli and Klebsiella pneumoniae. Identification of the episome was based on: (i) temperature sensitivity of the Lac+ and His+ phenotypes; (ii) the fact that F- segregants, obtained after temperature curing or acridine orange curing, were simultaneously Lac- and His-; and (iii) linkage of lac+ with his+ in episomal transfers to E. coli and S. typhimurium. The frequency of episome transfer was influenced by the genotype of the donor. Plasmid LT2, prevalent in S. typhimurium LT2 strains, was suggested to be responsible for the low fertility of S. typhimurium donors. Episome F42-400 was capable of chromosome mobilization, and the extent of chromosome mobilization was not influenced by the presence or absence of the histidine region on the donor chromosome. Growth in a defined medium with acridine orange was able to cure F42-400. The frequency of curing was increased (the frequency of His+ cells was 0.0001%) if the cells were grown at 40 C in the presence of acridine orange. Selection for temperature-resistant Lac+, His+ derivatives in a strain without histidine deletion yielded Hfr strains. However, similar and stronger selections in strains without the chromosomal histidine region failed to yield Hfr strains. Our inability to obtain Hfr's in strains without the chromosomal histidine region was explained by assuming that the episome F42-400 has lost the F sites involved in integration into the S. typhimurium chromosome."}
{"id": "PMID:1099077", "title": "Behavior of a hybrid F' ts114 lac+, his+ factor (F42-400) in Klebsiella pneumoniae M5a1.", "content": "Episome F' ts114 lac+, his+ (F42-400) was transferred from Salmonella typhimurium to Klebsiella pneumoniae. From the progeny, a strain of K. pneumoniae able to retransfer the episome was obtained. The His+ phenotype in this strain is temperature sensitive. Escherichia coli female-specific phages phiII, W31, and T3 were shown to plate on K. pneumoniae. From phiII we obtained two derivatives; phiIIK, which plates only on K. pneumoniae, and phiIIE, which plates only on E. coli. Growth of phages T3 and phiIIK was inhibited by F42-400 in K. pneumoniae. Growth in presence of acridine orange in a defined medium at 40 C resulted in a high level of curing. The frequency of His+ cells after growth in acridine orange at 40 C was 0.001%. An extensive search to detect chromosome mobilization by F42-400 in K. pneumoniae, under different experimental conditions, was negative. We cannot exclude the possibility that the low transfer efficiencies prevented our detection of chromosome mobilization. A search among temperature-resistant, acridine orange-curing-resistant, or galactose-resistant derivatives of the K. pneumoniae donor strain failed to reveal any chromosome transfer. Our failure to detect Hfr's may be a result of: (i) the peculiarity of episome F42-400, (ii) the peculiarity of K. pneumoniae chromosome, or (iii) low transfer efficiency. K. pneumoniae-modified F42-400 and phage 424 were restricted by E. Coli K-12. E. coli K-12-modified episome F42-400 and phage 424 were restricted by K. pneumoniae. E. coli C failed to restrict F42-400 modified with K. pneumoniae specificity. The ability of K. pneumoniae to accept F42-400 is less, by about a factor of 50, than that of E. coli C. As an explanation for the differences in the behavior of E. coli C and K. pneumoniae in ability to receive F42-400 it was suggested that recipient bacteria have specific sites for interaction with the F-pilus tip; these are present in E. Coli C, leading to high transfer efficiency, whereas they may not be present (or if present, are not accessible) in K. pneumoniae, leading to low transfer efficiency.", "contents": "Behavior of a hybrid F' ts114 lac+, his+ factor (F42-400) in Klebsiella pneumoniae M5a1. Episome F' ts114 lac+, his+ (F42-400) was transferred from Salmonella typhimurium to Klebsiella pneumoniae. From the progeny, a strain of K. pneumoniae able to retransfer the episome was obtained. The His+ phenotype in this strain is temperature sensitive. Escherichia coli female-specific phages phiII, W31, and T3 were shown to plate on K. pneumoniae. From phiII we obtained two derivatives; phiIIK, which plates only on K. pneumoniae, and phiIIE, which plates only on E. coli. Growth of phages T3 and phiIIK was inhibited by F42-400 in K. pneumoniae. Growth in presence of acridine orange in a defined medium at 40 C resulted in a high level of curing. The frequency of His+ cells after growth in acridine orange at 40 C was 0.001%. An extensive search to detect chromosome mobilization by F42-400 in K. pneumoniae, under different experimental conditions, was negative. We cannot exclude the possibility that the low transfer efficiencies prevented our detection of chromosome mobilization. A search among temperature-resistant, acridine orange-curing-resistant, or galactose-resistant derivatives of the K. pneumoniae donor strain failed to reveal any chromosome transfer. Our failure to detect Hfr's may be a result of: (i) the peculiarity of episome F42-400, (ii) the peculiarity of K. pneumoniae chromosome, or (iii) low transfer efficiency. K. pneumoniae-modified F42-400 and phage 424 were restricted by E. Coli K-12. E. coli K-12-modified episome F42-400 and phage 424 were restricted by K. pneumoniae. E. coli C failed to restrict F42-400 modified with K. pneumoniae specificity. The ability of K. pneumoniae to accept F42-400 is less, by about a factor of 50, than that of E. coli C. As an explanation for the differences in the behavior of E. coli C and K. pneumoniae in ability to receive F42-400 it was suggested that recipient bacteria have specific sites for interaction with the F-pilus tip; these are present in E. Coli C, leading to high transfer efficiency, whereas they may not be present (or if present, are not accessible) in K. pneumoniae, leading to low transfer efficiency."}
{"id": "PMID:1099078", "title": "Differences in coupling of energy to glycine and phenylalanine transport in aerobically grown Escherichia coli.", "content": "Differences exist in the coupling of energy to transport of glycine and phenylalanine in aerobically grown cells of Escherichia coli. Energy derived from respiration, although involved in both uptake systems, is not employed identically as shown by kinetic effects of cyanide and anoxia and by temperature dependencies. Additional evidence for aerobic differences was provided by the effects of azide which greatly decreased initial rates of uptake of glycine but not phenylalanine. The effect on glycine uptake was not due to uncoupling of oxidative phosphorylation or to a decrease in respiration rate. Evidence for anaerobic differences was provided by the addition of either glucose or ferricyanide to cell suspensions containing glycerol, thereby maintaining anoxic uptake of phenylalanine, but not glycine, at the aerobic level. Ferricyanide stimulation required a functional Ca, Mg-adenosine 5'-triphosphatase and involved cell metabolism. Ferricyanide was also found to produce differential stimulation of other amino acid transport systems; tyrosine, tryptophan and leucine uptakes were stimulated whereas those for alanine, proline, threonine, and glutamine were relatively unaffected.", "contents": "Differences in coupling of energy to glycine and phenylalanine transport in aerobically grown Escherichia coli. Differences exist in the coupling of energy to transport of glycine and phenylalanine in aerobically grown cells of Escherichia coli. Energy derived from respiration, although involved in both uptake systems, is not employed identically as shown by kinetic effects of cyanide and anoxia and by temperature dependencies. Additional evidence for aerobic differences was provided by the effects of azide which greatly decreased initial rates of uptake of glycine but not phenylalanine. The effect on glycine uptake was not due to uncoupling of oxidative phosphorylation or to a decrease in respiration rate. Evidence for anaerobic differences was provided by the addition of either glucose or ferricyanide to cell suspensions containing glycerol, thereby maintaining anoxic uptake of phenylalanine, but not glycine, at the aerobic level. Ferricyanide stimulation required a functional Ca, Mg-adenosine 5'-triphosphatase and involved cell metabolism. Ferricyanide was also found to produce differential stimulation of other amino acid transport systems; tyrosine, tryptophan and leucine uptakes were stimulated whereas those for alanine, proline, threonine, and glutamine were relatively unaffected."}
{"id": "PMID:1099079", "title": "Kinetic studies on the specificity of chelate-iron uptake in Aspergillus.", "content": "Three strains of the fungus Aspergillus, Aspergillus quadricinctus (E. Yuill), A. fumigatus (Fresenius), and A. melleus (Yukawa), each producing different iron-chelating compounds during iron-deficient cultivation, were used for 55Fe3+ uptake measurements. Iron from chelates of the ferrichrome-type family was taken up by young mycelia of all strains tested, irrespective of the ferrichrome-type compound these strains predominantly produce in low-iron cultures. Ferrichrysin-producing strains, however, seem to favor ferrichrysin iron uptake, whereas ferrichrome, ferricrocin, and even ferrirubin showed similar iron transport properties in all of these strains. Compared to iron uptake from ferrichrome-type compounds (Km approximately 4 uM) iron uptake from fusigen revealed completely different kinetic values (Km approximately 50 to 80 muM). Iron from exogenous chelates, e.g., from coprogen produced by Neurospora crassa for ferrioxamine B produced by Streptomyces pilosus, can obviously not be taken up by Aspergillus, confirming the pronounced specificity of chelate-iron transport in fungi.", "contents": "Kinetic studies on the specificity of chelate-iron uptake in Aspergillus. Three strains of the fungus Aspergillus, Aspergillus quadricinctus (E. Yuill), A. fumigatus (Fresenius), and A. melleus (Yukawa), each producing different iron-chelating compounds during iron-deficient cultivation, were used for 55Fe3+ uptake measurements. Iron from chelates of the ferrichrome-type family was taken up by young mycelia of all strains tested, irrespective of the ferrichrome-type compound these strains predominantly produce in low-iron cultures. Ferrichrysin-producing strains, however, seem to favor ferrichrysin iron uptake, whereas ferrichrome, ferricrocin, and even ferrirubin showed similar iron transport properties in all of these strains. Compared to iron uptake from ferrichrome-type compounds (Km approximately 4 uM) iron uptake from fusigen revealed completely different kinetic values (Km approximately 50 to 80 muM). Iron from exogenous chelates, e.g., from coprogen produced by Neurospora crassa for ferrioxamine B produced by Streptomyces pilosus, can obviously not be taken up by Aspergillus, confirming the pronounced specificity of chelate-iron transport in fungi."}
{"id": "PMID:1099081", "title": "Specificity of the Escherichia coli proline transport system.", "content": "The presence of both the carbonyl portion of the carboxyl group at position 2 of the pyrrolidine ring and a secondary amine was essential for uptake of a compound by the proline permease of Escherichia coli. The permease possessed a high affinity for azetidine-2-carboxylic acid and for compounds with ring structures smaller than the pyrrolidine ring. Pipecolic acid, the higher homologue of proline, and its derivatives were not transported. Cis- and trans-3,4-methano-prolines, also six-membered ring structures, behaved anomolously in that they possessed a high affinity for the permease. The difference between the methano-prolines and other six-membered ring structures probably resides in the fact that the former exist in the \"boat\" configuration whereas the latter possess the \"chair\" configuration. In general, substituted prolines in the cis configuration displayed a higher affinity for the permease than did corresponding trans isomers, though the affinity for substituted prolines was influenced by the position, size, and polar or nonpolar nature of the substituent group. At O C many analogues with affinity for proline permease exchanged with intracellular proline, but some analogues, notably trans-3-methyl- and trans-4-methyl-L-prolines, though possessing high affinity for the permease, showed an almost complete inability to exchange with intracellular proline.", "contents": "Specificity of the Escherichia coli proline transport system. The presence of both the carbonyl portion of the carboxyl group at position 2 of the pyrrolidine ring and a secondary amine was essential for uptake of a compound by the proline permease of Escherichia coli. The permease possessed a high affinity for azetidine-2-carboxylic acid and for compounds with ring structures smaller than the pyrrolidine ring. Pipecolic acid, the higher homologue of proline, and its derivatives were not transported. Cis- and trans-3,4-methano-prolines, also six-membered ring structures, behaved anomolously in that they possessed a high affinity for the permease. The difference between the methano-prolines and other six-membered ring structures probably resides in the fact that the former exist in the \"boat\" configuration whereas the latter possess the \"chair\" configuration. In general, substituted prolines in the cis configuration displayed a higher affinity for the permease than did corresponding trans isomers, though the affinity for substituted prolines was influenced by the position, size, and polar or nonpolar nature of the substituent group. At O C many analogues with affinity for proline permease exchanged with intracellular proline, but some analogues, notably trans-3-methyl- and trans-4-methyl-L-prolines, though possessing high affinity for the permease, showed an almost complete inability to exchange with intracellular proline."}
{"id": "PMID:1099080", "title": "Inhibition of Escherichia coli isoleucine biosynthesis by isoleucine tetrazole.", "content": "Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal of growth inhibiton was effected by L-leucine, L-methionine, or L-homoserine. The tetrazole inhibited the activity of the biosynthetic threonine deaminase (EC 4.2.1.16 L-threonine hydrolyase [deaminating]), the inhibition being relieved by L-valine. The tetrazole also inhibited isoleucyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.5 L-isoleucine: tRNA ligase [adenosine monophosphate]), but was without effect on the activities of alpha-isopropylmalate synthetase or acetohydroxy acid synthetase. One class of isoleucine tetrazole-resistant mutants produced biosynthetic threonine deaminases which were no longer subject to feedback inhibition by either isoleucine or the tetrazole.", "contents": "Inhibition of Escherichia coli isoleucine biosynthesis by isoleucine tetrazole. Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal of growth inhibiton was effected by L-leucine, L-methionine, or L-homoserine. The tetrazole inhibited the activity of the biosynthetic threonine deaminase (EC 4.2.1.16 L-threonine hydrolyase [deaminating]), the inhibition being relieved by L-valine. The tetrazole also inhibited isoleucyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.5 L-isoleucine: tRNA ligase [adenosine monophosphate]), but was without effect on the activities of alpha-isopropylmalate synthetase or acetohydroxy acid synthetase. One class of isoleucine tetrazole-resistant mutants produced biosynthetic threonine deaminases which were no longer subject to feedback inhibition by either isoleucine or the tetrazole."}
{"id": "PMID:1099082", "title": "Attachment of lipoprotein to murein (peptidoglycan) of Escherichia coli in the presence and absence of penicillin FL 1060.", "content": "In vivo studies on the attachment of lipoprotein to the murein (peptidoglycan) of Escherichia coli showed that it takes several generations of growth until the amount of lipoprotein on newly made murein is equilibrated. The technique used involves degradation of the sodium dodecyl sulfate-insoluble murein-lipoprotein complex (sacculus, rigid layer) with lysozyme and separation of the labeled products on paper. No lipoprotein was found on murein subunits incorporated during a pulse of [3H]diaminopimelate for 1 min in logarithmically growing cells at 37 C. Even after one doubling of the cell mass, only 4 to 8% of the labeled murein was isolated as bound to lipoprotein. With uniformly labeled murein, 30% remains bound to lipoprotein after lysozyme treatment, corresponding to three murein subunits. Therefore it can be concluded that during pulse labeling either no lipoprotein is incorporated into the newly synthesized murein or no murein subunits are inserted into existing murein around lipoprotein attachment sites. Longer pulse and pulse-chase experiments argue for the latter interpretation. It is therefore concluded that incorporation of murein subunits into the growing murein polymer is not at all a random process. Instead, quite large areas of murein, on which lipoprotein is situated, seem to be preserved. Under the influence of penicillin FL 1060 murein synthesis is 50% inhibited. The rate of lipoprotein attachment is less affected so that increasing amounts of lipoprotein become attached during spheroplast formation. By the time the stationary growth phase has been reached, the lipoprotein content of the murein has doubled. Diaminopimelate auxotrophic mutants require, in the presence of penicillin FL 1060, more diaminopimelate for full growth than in the absence of penicillin FL 1060. This finding and the fact that murein synthesis is always inhibited by 50% over a wide range of penicillin concentration (1 to 1,000 mug/ml) point to the inhibition of an enzymatic step of murein synthesis which can be partially bypassed by a second enzyme, less efficient but resistant to penicillin FL 1060.", "contents": "Attachment of lipoprotein to murein (peptidoglycan) of Escherichia coli in the presence and absence of penicillin FL 1060. In vivo studies on the attachment of lipoprotein to the murein (peptidoglycan) of Escherichia coli showed that it takes several generations of growth until the amount of lipoprotein on newly made murein is equilibrated. The technique used involves degradation of the sodium dodecyl sulfate-insoluble murein-lipoprotein complex (sacculus, rigid layer) with lysozyme and separation of the labeled products on paper. No lipoprotein was found on murein subunits incorporated during a pulse of [3H]diaminopimelate for 1 min in logarithmically growing cells at 37 C. Even after one doubling of the cell mass, only 4 to 8% of the labeled murein was isolated as bound to lipoprotein. With uniformly labeled murein, 30% remains bound to lipoprotein after lysozyme treatment, corresponding to three murein subunits. Therefore it can be concluded that during pulse labeling either no lipoprotein is incorporated into the newly synthesized murein or no murein subunits are inserted into existing murein around lipoprotein attachment sites. Longer pulse and pulse-chase experiments argue for the latter interpretation. It is therefore concluded that incorporation of murein subunits into the growing murein polymer is not at all a random process. Instead, quite large areas of murein, on which lipoprotein is situated, seem to be preserved. Under the influence of penicillin FL 1060 murein synthesis is 50% inhibited. The rate of lipoprotein attachment is less affected so that increasing amounts of lipoprotein become attached during spheroplast formation. By the time the stationary growth phase has been reached, the lipoprotein content of the murein has doubled. Diaminopimelate auxotrophic mutants require, in the presence of penicillin FL 1060, more diaminopimelate for full growth than in the absence of penicillin FL 1060. This finding and the fact that murein synthesis is always inhibited by 50% over a wide range of penicillin concentration (1 to 1,000 mug/ml) point to the inhibition of an enzymatic step of murein synthesis which can be partially bypassed by a second enzyme, less efficient but resistant to penicillin FL 1060."}
{"id": "PMID:1099083", "title": "Accumulation of arginine precursors in Escherichia coli: effects on growth, enzyme repression, and application to the forward selection of arginine auxotrophs.", "content": "The accumulation or ornithine, citrulline, and possibly acetylornithine by Escherichia coli K-12 arginineless mutants provided with acetylarginine as source of arginine causes severe growth inhibition. This occurs under conditions where comparable derivatives of E. coli W (Bollon and Vogel, 1973) show little or no growth inhibition. The same conditions, which have been reported to cause noncorrelative synthesis of acetylornithinase and argininosuccinase in E. coli W (Bollon and Vogel, 1973), do not alter the correlative pattern of enzyme synthesis observed in E. coli K-12. Moreover, previously reported effects of ornithine and citrulline on repression of the arginine regulon in E. coli W are not observed in the K-12 strains examined. The bearing of these observations on possible differences between the mechanism of enzyme repression operating in the two types of strains cannot yet be fully evaluated; it is, however, clear that considerable care should be exercised before extrapolating the results obtained with one type of strain to the other one. The particularly strong inhibition of acetylarginine utilization exerted by ornithine in E. coli K-12 allows the forward selection of several classes of arginine auxotrophs from strains deficient in carbamoylphosphate biosynthesis and thus capable of ornithine accumulation. Possible applications of this technique to the genetic analysis of the bipolar argECBH operon are discussed.", "contents": "Accumulation of arginine precursors in Escherichia coli: effects on growth, enzyme repression, and application to the forward selection of arginine auxotrophs. The accumulation or ornithine, citrulline, and possibly acetylornithine by Escherichia coli K-12 arginineless mutants provided with acetylarginine as source of arginine causes severe growth inhibition. This occurs under conditions where comparable derivatives of E. coli W (Bollon and Vogel, 1973) show little or no growth inhibition. The same conditions, which have been reported to cause noncorrelative synthesis of acetylornithinase and argininosuccinase in E. coli W (Bollon and Vogel, 1973), do not alter the correlative pattern of enzyme synthesis observed in E. coli K-12. Moreover, previously reported effects of ornithine and citrulline on repression of the arginine regulon in E. coli W are not observed in the K-12 strains examined. The bearing of these observations on possible differences between the mechanism of enzyme repression operating in the two types of strains cannot yet be fully evaluated; it is, however, clear that considerable care should be exercised before extrapolating the results obtained with one type of strain to the other one. The particularly strong inhibition of acetylarginine utilization exerted by ornithine in E. coli K-12 allows the forward selection of several classes of arginine auxotrophs from strains deficient in carbamoylphosphate biosynthesis and thus capable of ornithine accumulation. Possible applications of this technique to the genetic analysis of the bipolar argECBH operon are discussed."}
{"id": "PMID:1099084", "title": "Role of the cell surface in bacterial mating: requirement for intact mucopeptide in donors for the expression of surface exclusion in R+ strains of Escherichia coli.", "content": "Two derivatives of the F-like R factor R1drd19 carrying mutually exclusive resistance determinants were used to study the role of the mucopeptide in the expression of conjugal functions. The use of metabolically active penicillin spheroplasts in R+ times R- matings had no effect on the ability of the cells to donate or accept a plasmid. However, in R+ times R+ matings it was found that surface exclusion was totally abolished if the donor, but not the recipient, was a spheroplast. This result implies that the traS gene, expressed by the excluding plasmid, is dependent for its action on an intact mucopeptide layer in the donor cell, and that this interaction is independent of the transfer ability of the excluding plasmid.", "contents": "Role of the cell surface in bacterial mating: requirement for intact mucopeptide in donors for the expression of surface exclusion in R+ strains of Escherichia coli. Two derivatives of the F-like R factor R1drd19 carrying mutually exclusive resistance determinants were used to study the role of the mucopeptide in the expression of conjugal functions. The use of metabolically active penicillin spheroplasts in R+ times R- matings had no effect on the ability of the cells to donate or accept a plasmid. However, in R+ times R+ matings it was found that surface exclusion was totally abolished if the donor, but not the recipient, was a spheroplast. This result implies that the traS gene, expressed by the excluding plasmid, is dependent for its action on an intact mucopeptide layer in the donor cell, and that this interaction is independent of the transfer ability of the excluding plasmid."}
{"id": "PMID:1099085", "title": "Membrane-associated nucleotide sugar reactions: influence of mutations affecting lipopolysaccharide on the first enzyme of O-antigen synthesis.", "content": "Both the synthesis of lipopolysaccharide O-antigen and the synthesis of peptidoglycan in Salmonella typhimurium proceed via membrane-bound glycosylated lipid intermediates. The first enzyme of each pathway transfers a sugar phosphate from a nucleotide sugar to the glycosyl carrier lipid (P-GCL). Each enzyme catalyzes an exchange reaction between the reaction product urine monophosphate, and the nucleotide sugar substrate. Several strains of S. typhimurium defective in lipopolysaccharide synthesis accumulate glycosylated lipid intermediates under appropriate conditions. In addition, strains lysogenic for phage P22 synthesize a glucose derivative of the carrier lipid. These strains were used to demonstrate the P/GCL requirement of the exchange reaction catalyzed by galactose-diphosphoglycosyl carrier lipid (GCL-PP-Gal) synthetase, the first enzyme of O-antigen synthesis. Enzyme activity is greatly reduced when glycosylated P-GCL accumulates on the cytoplasmic membrane. The exchange reaction catalyzed by the first enzyme of peptidoglycan synthesis is unaffected by the accumulation of O-antigen fragments on the carrier lipid and may interact with a different pool of P-GCL within the membrane. GCL-PP-Gal synthetase activity cannot be detected in the membranes of two rfa mutants that synthesize incomplete lipopolysaccharide core. Either the synthesis of GCL-PP-Gal synthetase or the stable integration of the enzyme into the membrane structure may be disrupted in the rfa mutants. Peptidoglycan synthesis is unaffected by the mutations affecting the core glycosyltransferases.", "contents": "Membrane-associated nucleotide sugar reactions: influence of mutations affecting lipopolysaccharide on the first enzyme of O-antigen synthesis. Both the synthesis of lipopolysaccharide O-antigen and the synthesis of peptidoglycan in Salmonella typhimurium proceed via membrane-bound glycosylated lipid intermediates. The first enzyme of each pathway transfers a sugar phosphate from a nucleotide sugar to the glycosyl carrier lipid (P-GCL). Each enzyme catalyzes an exchange reaction between the reaction product urine monophosphate, and the nucleotide sugar substrate. Several strains of S. typhimurium defective in lipopolysaccharide synthesis accumulate glycosylated lipid intermediates under appropriate conditions. In addition, strains lysogenic for phage P22 synthesize a glucose derivative of the carrier lipid. These strains were used to demonstrate the P/GCL requirement of the exchange reaction catalyzed by galactose-diphosphoglycosyl carrier lipid (GCL-PP-Gal) synthetase, the first enzyme of O-antigen synthesis. Enzyme activity is greatly reduced when glycosylated P-GCL accumulates on the cytoplasmic membrane. The exchange reaction catalyzed by the first enzyme of peptidoglycan synthesis is unaffected by the accumulation of O-antigen fragments on the carrier lipid and may interact with a different pool of P-GCL within the membrane. GCL-PP-Gal synthetase activity cannot be detected in the membranes of two rfa mutants that synthesize incomplete lipopolysaccharide core. Either the synthesis of GCL-PP-Gal synthetase or the stable integration of the enzyme into the membrane structure may be disrupted in the rfa mutants. Peptidoglycan synthesis is unaffected by the mutations affecting the core glycosyltransferases."}
{"id": "PMID:1099086", "title": "Effect of temperature on ergosterol biosynthesis in yeast.", "content": "Anaerobically grown Saccharomyces cerevisiae was aerated for 7 hr at 20 degrees, 30 degrees, or 40 degrees, in a phosphate buffer containing 2% glucose. At elevated temperature (40 degrees), de novo synthesis of squalene and sterois in the aerated yeast was only 32-35% of that at lower temperature (20 degrees or 30 degrees), and this decrease was attributed to the repression of the enzymes involved in the synthesis of mevalonate from acetyl-COA. In addition, at elevated temperature, the metabolic flux from squalene to ergosterol was blocked at squalene epoxidation, lanosterol demethylation, and ergosta-5, 7, 22, 24(28)-tetraene-3beta-ol reduction.", "contents": "Effect of temperature on ergosterol biosynthesis in yeast. Anaerobically grown Saccharomyces cerevisiae was aerated for 7 hr at 20 degrees, 30 degrees, or 40 degrees, in a phosphate buffer containing 2% glucose. At elevated temperature (40 degrees), de novo synthesis of squalene and sterois in the aerated yeast was only 32-35% of that at lower temperature (20 degrees or 30 degrees), and this decrease was attributed to the repression of the enzymes involved in the synthesis of mevalonate from acetyl-COA. In addition, at elevated temperature, the metabolic flux from squalene to ergosterol was blocked at squalene epoxidation, lanosterol demethylation, and ergosta-5, 7, 22, 24(28)-tetraene-3beta-ol reduction."}
{"id": "PMID:1099087", "title": "Conformational transitions of polypeptide chain elongation factor Tu. I. Studies with hydrophobic probes.", "content": "The conformational difference between polypeptide chain elongation factor Tu (EF-Tu)-GTP and EF-Tu-GDP has been studied using hydrophobic and fluorescent probes. The interaction of EF-Tu-GDP with 1-anilino-8-naphthalenesulfonate (ANS) was measured in terms of the enhancement of the fluorescence intensity at the emission maximum of 475 nm. When EF-Tu-GDP-ANS complex was converted to EF-Tu-GTP-ANS complex by incubation with phosphoenolpyruvate and pyruvate kinase [EC 2.7.1.40], there was a roughly 2-fold increase in fluorescence intensity and a blue shift of the emission maximum from 475 to 467 nm, indicating a conformational transition of the protein. The conformational change was found to be reversible and the spectrum promptly returned to that of EF-Tu-GDP-ANS complex upon addition of excess GDP. A similar change in the spectrum was also observed when aminoacyl-tRNA, but not deacylated tRNA, was added to EF-Tu-GDP-ANS complex. Measurement of the number of binding sites by gel filtration indicated that EF-Tu-GTP and EF-Tu-GDP bind 2.9 and 1.7 molecules of ANS, respectively. These results suggest that in EF-Tu-GTP the conformation was altered and one additional binding site for ANS was created at or near the site interacting with aminoacyl-tRNA. Another reagent, N-(1-anilinonaphthyl-4) maleimide (ANM) was covalently bound to the sulfhydryl group in EF-Tu-GDP which is essential for interaction with aminoacyl-tRNA. The binding could be determined spectrofluorometrically, since the reagent, which is nonfluorescent in aqueous solution, emitted a strong fluorescence upon binding with the sulfhydryl group, indicating a marked hydrophobicity of the local environment. Measurements of the kinetics of the binding revealed that ANM reacted rapidly with the sulfhydryl group in EF-Tu-GTP, while the reaction with that in EF-Tu-GDP proceeded more sluggishly. The difference in the reactivity of the sulfhydryl group essential for aminoacyl-tRNA binding between EF-Tu-GTP and EF-Tu-GDP probably reflects a conformational transition of the protein near the active site. These results, together with those on spin-label studies previously published (Arai, Kawakita, Kaziro, Maeda, & Onishi (1974) J. Biol. Chem. 249, 3311), demonstrate that reversible conformational transition does occur in EF-Tu on changing the ligand from GDP to GTP.", "contents": "Conformational transitions of polypeptide chain elongation factor Tu. I. Studies with hydrophobic probes. The conformational difference between polypeptide chain elongation factor Tu (EF-Tu)-GTP and EF-Tu-GDP has been studied using hydrophobic and fluorescent probes. The interaction of EF-Tu-GDP with 1-anilino-8-naphthalenesulfonate (ANS) was measured in terms of the enhancement of the fluorescence intensity at the emission maximum of 475 nm. When EF-Tu-GDP-ANS complex was converted to EF-Tu-GTP-ANS complex by incubation with phosphoenolpyruvate and pyruvate kinase [EC 2.7.1.40], there was a roughly 2-fold increase in fluorescence intensity and a blue shift of the emission maximum from 475 to 467 nm, indicating a conformational transition of the protein. The conformational change was found to be reversible and the spectrum promptly returned to that of EF-Tu-GDP-ANS complex upon addition of excess GDP. A similar change in the spectrum was also observed when aminoacyl-tRNA, but not deacylated tRNA, was added to EF-Tu-GDP-ANS complex. Measurement of the number of binding sites by gel filtration indicated that EF-Tu-GTP and EF-Tu-GDP bind 2.9 and 1.7 molecules of ANS, respectively. These results suggest that in EF-Tu-GTP the conformation was altered and one additional binding site for ANS was created at or near the site interacting with aminoacyl-tRNA. Another reagent, N-(1-anilinonaphthyl-4) maleimide (ANM) was covalently bound to the sulfhydryl group in EF-Tu-GDP which is essential for interaction with aminoacyl-tRNA. The binding could be determined spectrofluorometrically, since the reagent, which is nonfluorescent in aqueous solution, emitted a strong fluorescence upon binding with the sulfhydryl group, indicating a marked hydrophobicity of the local environment. Measurements of the kinetics of the binding revealed that ANM reacted rapidly with the sulfhydryl group in EF-Tu-GTP, while the reaction with that in EF-Tu-GDP proceeded more sluggishly. The difference in the reactivity of the sulfhydryl group essential for aminoacyl-tRNA binding between EF-Tu-GTP and EF-Tu-GDP probably reflects a conformational transition of the protein near the active site. These results, together with those on spin-label studies previously published (Arai, Kawakita, Kaziro, Maeda, & Onishi (1974) J. Biol. Chem. 249, 3311), demonstrate that reversible conformational transition does occur in EF-Tu on changing the ligand from GDP to GTP."}
{"id": "PMID:1099088", "title": "Crystallographic studies on L-asparaginase from Proteus vulgaris. II. Symmetry and location of the tetrameric molecule.", "content": "Analyses of the x-ray diffraction intensity data by the Patterson synthesis and rotation function techniques show that the true space group of the monoclinic crystals of L-asparaginase (L-asparagine amidohydrolase, EC 3.5.1.1) from Proteus vulgaris is P21, that the molecular centers lie at x = 0.054, y = 0, z = 0.256, and its symmetry related positions, and that the tetramer molecules possess three approximate, mutually perpendicular 2-fold rotational symmetries, the axes of which run along the directions of the crystallographic a*-, b-, and c-axes. In addition, an investigation of the molecular packing arrangement in the crystal indicates that the tetramer molecules possess an approximately regular tetrahedral subunit structure.", "contents": "Crystallographic studies on L-asparaginase from Proteus vulgaris. II. Symmetry and location of the tetrameric molecule. Analyses of the x-ray diffraction intensity data by the Patterson synthesis and rotation function techniques show that the true space group of the monoclinic crystals of L-asparaginase (L-asparagine amidohydrolase, EC 3.5.1.1) from Proteus vulgaris is P21, that the molecular centers lie at x = 0.054, y = 0, z = 0.256, and its symmetry related positions, and that the tetramer molecules possess three approximate, mutually perpendicular 2-fold rotational symmetries, the axes of which run along the directions of the crystallographic a*-, b-, and c-axes. In addition, an investigation of the molecular packing arrangement in the crystal indicates that the tetramer molecules possess an approximately regular tetrahedral subunit structure."}
{"id": "PMID:1099089", "title": "In vitro synthesis of transfer RNA. I. Purification of required components.", "content": "We have described an in vitro system in which active su+III tRNATyr is synthesized from a phi80psu++III DNA template. Using this system, we have identified four essential components that are required for synthesis of tRNA. The first of these is DNA-dependent RNA polymerase. It has been shown that a crude preparation of DNA-dependent RNA polymerase synthesizes su++III tRNATyr precursor similar to that which has been isolated in vivo, and that this preparation is capable of supporting high levels of tRNA synthesis. With purified DNA-dependent RNA polymerase, the su++III tRNATyr precursor was not observed as a transcription product and tRNA synthesis was below detetable levels. On this basis, a second essential component for tRNA synthesis was identified. This fraction, designated Fraction V, in combination with purified RNA polymerase, catalyzes the synthesis of precursor tRNA. The third component is a ribonuclease (RNase P III), which specifically catalyzes the removal of the extra nucleotides present at the 3' terminus of the tRNA precursor. In the absence of this fraction, the in vitro synthesized su++III tRNATyr is slightly larger than 4 S and contains additional nucleotides beyond the normal --CCAOH 3 terminus of the mature tRNA. The fourth essential component required is a fraction containing RNase P, a previously identified endonuclease which specifically catalyzes the removal of the 5' extra nucleotides present on tRNA precursors.", "contents": "In vitro synthesis of transfer RNA. I. Purification of required components. We have described an in vitro system in which active su+III tRNATyr is synthesized from a phi80psu++III DNA template. Using this system, we have identified four essential components that are required for synthesis of tRNA. The first of these is DNA-dependent RNA polymerase. It has been shown that a crude preparation of DNA-dependent RNA polymerase synthesizes su++III tRNATyr precursor similar to that which has been isolated in vivo, and that this preparation is capable of supporting high levels of tRNA synthesis. With purified DNA-dependent RNA polymerase, the su++III tRNATyr precursor was not observed as a transcription product and tRNA synthesis was below detetable levels. On this basis, a second essential component for tRNA synthesis was identified. This fraction, designated Fraction V, in combination with purified RNA polymerase, catalyzes the synthesis of precursor tRNA. The third component is a ribonuclease (RNase P III), which specifically catalyzes the removal of the extra nucleotides present at the 3' terminus of the tRNA precursor. In the absence of this fraction, the in vitro synthesized su++III tRNATyr is slightly larger than 4 S and contains additional nucleotides beyond the normal --CCAOH 3 terminus of the mature tRNA. The fourth essential component required is a fraction containing RNase P, a previously identified endonuclease which specifically catalyzes the removal of the 5' extra nucleotides present on tRNA precursors."}
{"id": "PMID:1099090", "title": "In vitro synthesis of transfer RNA. II. Identification of required enzymatic activities.", "content": "We have shown that the synthesis of active su+III tRNATyr from a phi80psu+III DNA template requires the action of four distinct enzymatic activities. The first of these, DNA-dependent RNA polymerase, catalyzes the formation of a large molecular weight transcript, initiating synthesis at a specific site 41 nucleotides proximal to the 5' end of the su+III tRNATyr structural gene and continuing at least 100 nucleotides beyond the 3' terminus of the su+III tRNATyr sequence. The second required component, designated Fraction V, allows purified DNA-DEPENDENT RNA polymerase to function in tRNA synthesis. We have shown that this fraction contains an endonuclease that together with DNA-dependent RNA polymerase is responsible for the synthesis of su+III tRNATyr \"precursor\". Thus, su+III tRNATyr precursor is not itself the primary transcription product of the su+III tRNATyr gene, but rather, it arises as a result of post-transcriptional cleavage of a much larger transcript by the action of the nuclease present in Fraction V. The third enzymatic activity required for synthesis of active su+III tRNATyr is a ribonuclease (RNase P III) that specifically catalyzes the removal of the 3' extra nucleotides from the su+III tRNATyr precursor. The fourth activity required for synthesis of tRNA is a previously identified endonuclease, RNase P, that specifically catalyzes the removal of the 5' extra nucleotides from tRNA precursors. The properties of RNase P purified according to the procedure developed in this laboratory have been compared with those of the enzyme purified from ribosomes according to the procedure described by Robertson et al. (Robertson, H.D., Altman, S., and Smith, F.D. (1972) J.Biol. Chem. 247, 5243-5251.).", "contents": "In vitro synthesis of transfer RNA. II. Identification of required enzymatic activities. We have shown that the synthesis of active su+III tRNATyr from a phi80psu+III DNA template requires the action of four distinct enzymatic activities. The first of these, DNA-dependent RNA polymerase, catalyzes the formation of a large molecular weight transcript, initiating synthesis at a specific site 41 nucleotides proximal to the 5' end of the su+III tRNATyr structural gene and continuing at least 100 nucleotides beyond the 3' terminus of the su+III tRNATyr sequence. The second required component, designated Fraction V, allows purified DNA-DEPENDENT RNA polymerase to function in tRNA synthesis. We have shown that this fraction contains an endonuclease that together with DNA-dependent RNA polymerase is responsible for the synthesis of su+III tRNATyr \"precursor\". Thus, su+III tRNATyr precursor is not itself the primary transcription product of the su+III tRNATyr gene, but rather, it arises as a result of post-transcriptional cleavage of a much larger transcript by the action of the nuclease present in Fraction V. The third enzymatic activity required for synthesis of active su+III tRNATyr is a ribonuclease (RNase P III) that specifically catalyzes the removal of the 3' extra nucleotides from the su+III tRNATyr precursor. The fourth activity required for synthesis of tRNA is a previously identified endonuclease, RNase P, that specifically catalyzes the removal of the 5' extra nucleotides from tRNA precursors. The properties of RNase P purified according to the procedure developed in this laboratory have been compared with those of the enzyme purified from ribosomes according to the procedure described by Robertson et al. (Robertson, H.D., Altman, S., and Smith, F.D. (1972) J.Biol. Chem. 247, 5243-5251.)."}
{"id": "PMID:1099091", "title": "Structure of an exophthalmos-producing factor derived from thyrotropin by partial pepsin digestion.", "content": "Previously reported experiments (Winand, R.J., and Kohn, L.D. (1970) J. Biol. Chem. 245, 967-975; Kohn, L.D., and Winand, R.J. (1971) J. Biol. Chem 246, 6570-6575) have demonstrated that partial pepsin digestion of bovine thyrotropin preparation yields a fragment of the thyrotropin molecule which is exophthalmogenic but has negligible or no thyroid-stimulating activity. In the present report this exophthalmogenic derivative of the thyrotropin molecule is shown to contain two major polypeptide components with approximate molecular weights of 14,000 and 6,000. Amino acid analyses, carbohydrate analyses, and tryptic digestion experiments indicate that this exophthalmogenic factor is composed of an intact or nearly intact beta subunit of thyrotropin and an NH2-terminal fragment of the alpha subunit of thyrotropin. Neither polypeptide component of the exophthalmogenic factor has the in vivo exophthalmogenic activity of the intact structure. In vitro the intact exophthalmogenic derivative of the thyrotropin molecule can bind to the thyrotropin receptor on thyroid membranes less efficiently than thyrotropin but significantly better than either its own polypeptide components or the alpha or beta subunits of thyrotropin. The exophthalmogenic factor and its parent thyrotropin molecule can stimulate adenylate cyclase activity in retro-orbital tissue membranes from guinea pigs, a mammalian model of exophthalmos; its polypeptide components have little or no such activity.", "contents": "Structure of an exophthalmos-producing factor derived from thyrotropin by partial pepsin digestion. Previously reported experiments (Winand, R.J., and Kohn, L.D. (1970) J. Biol. Chem. 245, 967-975; Kohn, L.D., and Winand, R.J. (1971) J. Biol. Chem 246, 6570-6575) have demonstrated that partial pepsin digestion of bovine thyrotropin preparation yields a fragment of the thyrotropin molecule which is exophthalmogenic but has negligible or no thyroid-stimulating activity. In the present report this exophthalmogenic derivative of the thyrotropin molecule is shown to contain two major polypeptide components with approximate molecular weights of 14,000 and 6,000. Amino acid analyses, carbohydrate analyses, and tryptic digestion experiments indicate that this exophthalmogenic factor is composed of an intact or nearly intact beta subunit of thyrotropin and an NH2-terminal fragment of the alpha subunit of thyrotropin. Neither polypeptide component of the exophthalmogenic factor has the in vivo exophthalmogenic activity of the intact structure. In vitro the intact exophthalmogenic derivative of the thyrotropin molecule can bind to the thyrotropin receptor on thyroid membranes less efficiently than thyrotropin but significantly better than either its own polypeptide components or the alpha or beta subunits of thyrotropin. The exophthalmogenic factor and its parent thyrotropin molecule can stimulate adenylate cyclase activity in retro-orbital tissue membranes from guinea pigs, a mammalian model of exophthalmos; its polypeptide components have little or no such activity."}
{"id": "PMID:1099092", "title": "Enhancement of migration inhibitory factor activity by plasma esterase inhibitors.", "content": "The plasma esterase inhibitors alpha2-macroglobulin, alpha1-antitrypsin, C1-inhibitor, antithrombin-heparin cofactor, and, as previously described, soybean trypsin inhibitor (Kunitz) and diisopropylphosphorofluoridate (9) enhance the response of guinea pig macrophages to migration inhibitory factor. To obtain this effect, macrophages are incubated with inhibitors prior to assay. The data suggest that (a) the enhancement of migration inhibitory factor response is due to the inhibition of esterases associated with the macrophage through a distinct active site on the inhibitors, and (b) that the active sites of antithrombin-heparin cofactor and soybean trypsin inhibitor, which interact with the macrophage enzymes, are different from the active sites of these inhibitors which interact with thrombin and trypsin respectively. Chemical modification of the active site of antithrombin-heparin cofactor for thrombin and of soybean trypsin inhibitor for trypsin does not affect their capacity to enhance the migration inhibitory factor response. From studies with thrombin, it was known that antithrombin-heparin cofactor has a heparin binding site. Addition of heparin was found to prevent the migration inhibitory factor-enhancing effect of antithrombin-heparin cofactor. The present results suggest that plasma esterase inhibitors may play a regulatory role in the response of macrophages to mediators of cellular immunity.", "contents": "Enhancement of migration inhibitory factor activity by plasma esterase inhibitors. The plasma esterase inhibitors alpha2-macroglobulin, alpha1-antitrypsin, C1-inhibitor, antithrombin-heparin cofactor, and, as previously described, soybean trypsin inhibitor (Kunitz) and diisopropylphosphorofluoridate (9) enhance the response of guinea pig macrophages to migration inhibitory factor. To obtain this effect, macrophages are incubated with inhibitors prior to assay. The data suggest that (a) the enhancement of migration inhibitory factor response is due to the inhibition of esterases associated with the macrophage through a distinct active site on the inhibitors, and (b) that the active sites of antithrombin-heparin cofactor and soybean trypsin inhibitor, which interact with the macrophage enzymes, are different from the active sites of these inhibitors which interact with thrombin and trypsin respectively. Chemical modification of the active site of antithrombin-heparin cofactor for thrombin and of soybean trypsin inhibitor for trypsin does not affect their capacity to enhance the migration inhibitory factor response. From studies with thrombin, it was known that antithrombin-heparin cofactor has a heparin binding site. Addition of heparin was found to prevent the migration inhibitory factor-enhancing effect of antithrombin-heparin cofactor. The present results suggest that plasma esterase inhibitors may play a regulatory role in the response of macrophages to mediators of cellular immunity."}
{"id": "PMID:1099093", "title": "The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli.", "content": "The membrane-bound formate dehydrogenase of Escherichia coli grown anaerobically in the presence of nitrate was solubilized with deoxycholate and purified to near homogeneity. The purification procedure included ammonium sulfate fractionation and chromatography on Bio-Gel A-1.5m and DEAE Bio-Gel A in the presence of the nonionic detergent, Triton X-100. This detergent caused a significant decrease in the molecular weight of the soluble formate dehydrogenase complex and allowed the enzyme then to be resolved from other membrane components. Anaerobic conditions were required throughout due to the sensitivity of the enzyme to oxygen inactivation. Formate dehydrogenase was judged to be at least 93 to 99% pure by the following procedures: polyacrylamide gel electrophoresis in the presence of Triton X-100 and sodium dodecyl sulfate, gel filtration, and sedimentation velocity studies. The purified enzyme exists as a detergent-protein complex (0.20 +/- 0.03 g of Triton X-100/g of protein) which has an S20,w of 18.1 S and a Stokes radius of 76 A. This corresponds to a molecular weight of 590,000 +/- 59,000. The enzyme had an absorbance spectrum of a b-type cytochrome which could be completely reduced by formate. The heme content corresponds to an equivalent weight of 154,000 which suggests a tetrameric structure for the enzyme. Formate dehydrogenase was found to contain (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide. Neither FAD nor FMN could be detected. The enzyme contains three polypeptides, designated alpha, beta, and gamma, whose molecular weights were estimated by gel electrophoresis in the presence of sodium dodecyl sulfate to be 110,000, 32,000, and 20,000, respectively. After separation of the polypeptides by gel filtration in the presence of sodium dodecyl sulfate alpha, beta, and gamma were found in 1:1.2:0.55 molar ratios. A study of the enzyme obtained from cells grown with [75Se]selenite showed that only the alpha polypeptide contained significant amounts of selenium. The enzyme will catalyze the formate-dependent reduction of phenazine methosulfate, dichlorophenolindophenol, methylene blue, nitroblue tetrazolium, benzyl viologen, methyl viologen, ferricyanide, and coenzyme Q6. Cyanide, azide, p-hydroxymercuribenzoate, iodoacetamide, and oxygen inhibit the enzyme. The procedure which was designed for the purification of formate dehydrogenase also yields a highly purified preparation of nitrate reductase. This nitrate reductase has been shown to contain significant amounts of heme (Enoch, H. G., and Lester, R. L. (1974) Biochem. Biophys. Res Commun. 61,1234-1241). The enzyme contains three polypeptides with molecular weights of 155,000, 63,000, and 19,000. When measured in the presence of Trition X-100 the Stokes radius of nitrate reductase is 75 A and the S20,w is 16 S which corresponds to a molecular weight of 498,000.", "contents": "The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli. The membrane-bound formate dehydrogenase of Escherichia coli grown anaerobically in the presence of nitrate was solubilized with deoxycholate and purified to near homogeneity. The purification procedure included ammonium sulfate fractionation and chromatography on Bio-Gel A-1.5m and DEAE Bio-Gel A in the presence of the nonionic detergent, Triton X-100. This detergent caused a significant decrease in the molecular weight of the soluble formate dehydrogenase complex and allowed the enzyme then to be resolved from other membrane components. Anaerobic conditions were required throughout due to the sensitivity of the enzyme to oxygen inactivation. Formate dehydrogenase was judged to be at least 93 to 99% pure by the following procedures: polyacrylamide gel electrophoresis in the presence of Triton X-100 and sodium dodecyl sulfate, gel filtration, and sedimentation velocity studies. The purified enzyme exists as a detergent-protein complex (0.20 +/- 0.03 g of Triton X-100/g of protein) which has an S20,w of 18.1 S and a Stokes radius of 76 A. This corresponds to a molecular weight of 590,000 +/- 59,000. The enzyme had an absorbance spectrum of a b-type cytochrome which could be completely reduced by formate. The heme content corresponds to an equivalent weight of 154,000 which suggests a tetrameric structure for the enzyme. Formate dehydrogenase was found to contain (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide. Neither FAD nor FMN could be detected. The enzyme contains three polypeptides, designated alpha, beta, and gamma, whose molecular weights were estimated by gel electrophoresis in the presence of sodium dodecyl sulfate to be 110,000, 32,000, and 20,000, respectively. After separation of the polypeptides by gel filtration in the presence of sodium dodecyl sulfate alpha, beta, and gamma were found in 1:1.2:0.55 molar ratios. A study of the enzyme obtained from cells grown with [75Se]selenite showed that only the alpha polypeptide contained significant amounts of selenium. The enzyme will catalyze the formate-dependent reduction of phenazine methosulfate, dichlorophenolindophenol, methylene blue, nitroblue tetrazolium, benzyl viologen, methyl viologen, ferricyanide, and coenzyme Q6. Cyanide, azide, p-hydroxymercuribenzoate, iodoacetamide, and oxygen inhibit the enzyme. The procedure which was designed for the purification of formate dehydrogenase also yields a highly purified preparation of nitrate reductase. This nitrate reductase has been shown to contain significant amounts of heme (Enoch, H. G., and Lester, R. L. (1974) Biochem. Biophys. Res Commun. 61,1234-1241). The enzyme contains three polypeptides with molecular weights of 155,000, 63,000, and 19,000. When measured in the presence of Trition X-100 the Stokes radius of nitrate reductase is 75 A and the S20,w is 16 S which corresponds to a molecular weight of 498,000."}
{"id": "PMID:1099094", "title": "Anaerobic transport in Escherichia coli membrane vesicles.", "content": "Anaerobic lactose and/or amino acid transport by membrane vesicles prepared from Escherichia coli ML 308-225 can be coupled to at least four electron transfer systems: alpha-glycerol-P-dehydrogenase:nitrate reductase, formate dehydrogenase:nitrate reductase, alpha-glycerol-P dehydrogenase:fumarate reductase, and formate dehydrogenase:fumarate reductase. Vesicles contain one or more of these electron transfer systems depending on the growth conditions of the parent cells. alpha-Glycerol-P dehydrogenase and fumarate reductase are present only in vesicles prepared from cells grown in the presence of glycerol or fumarate, respectively. Formate dehydrogenase and nitrate reductase activities, on the other hand, are present in vesicles from cells grown on a variety of media. alpha-Glycerol-P and formate are able to drive aerobic transport in vesicles prepared from anaerobically grown cells, indicating coupling between aerobic and anaerobic electron transfer systems.", "contents": "Anaerobic transport in Escherichia coli membrane vesicles. Anaerobic lactose and/or amino acid transport by membrane vesicles prepared from Escherichia coli ML 308-225 can be coupled to at least four electron transfer systems: alpha-glycerol-P-dehydrogenase:nitrate reductase, formate dehydrogenase:nitrate reductase, alpha-glycerol-P dehydrogenase:fumarate reductase, and formate dehydrogenase:fumarate reductase. Vesicles contain one or more of these electron transfer systems depending on the growth conditions of the parent cells. alpha-Glycerol-P dehydrogenase and fumarate reductase are present only in vesicles prepared from cells grown in the presence of glycerol or fumarate, respectively. Formate dehydrogenase and nitrate reductase activities, on the other hand, are present in vesicles from cells grown on a variety of media. alpha-Glycerol-P and formate are able to drive aerobic transport in vesicles prepared from anaerobically grown cells, indicating coupling between aerobic and anaerobic electron transfer systems."}
{"id": "PMID:1099095", "title": "Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with an impermeant azidophenylgalactoside.", "content": "2'-N-(2-Nitro-4-azidophenyl) aminoethyl-1-thio-beta-D-galactopyranoside (APG2) is a competitive inhibitor of lactose transport in membrane vesicles isolated from Escherichia coli ML 308-225, exhibiting an apparent Ki of 30 to 40 muM, but is not transported. When irradiated with visible light in the presence of D-lactate, APG2 irreversibly inactivates the lac transport system. Imposition of a membrane potential (positive outside) by valinomycin-induced potassium efflux also causes APG2 photoinactivation. Strikingly, photoinactivation is not observed in the absence of D-lactate or a potassium diffusion gradient. Kinetic studies of the inactivation process yield a KD of 35 muM. Since lactose protects against the inactivation, it is apparent that these effects are specific for the lac transport system. The results show that APG2 inactivates from the outer surface of the vesicle membrane and support the previous hypothesis that the lac carrier protein is unable to bind external substrate in the absence of energy coupling.", "contents": "Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with an impermeant azidophenylgalactoside. 2'-N-(2-Nitro-4-azidophenyl) aminoethyl-1-thio-beta-D-galactopyranoside (APG2) is a competitive inhibitor of lactose transport in membrane vesicles isolated from Escherichia coli ML 308-225, exhibiting an apparent Ki of 30 to 40 muM, but is not transported. When irradiated with visible light in the presence of D-lactate, APG2 irreversibly inactivates the lac transport system. Imposition of a membrane potential (positive outside) by valinomycin-induced potassium efflux also causes APG2 photoinactivation. Strikingly, photoinactivation is not observed in the absence of D-lactate or a potassium diffusion gradient. Kinetic studies of the inactivation process yield a KD of 35 muM. Since lactose protects against the inactivation, it is apparent that these effects are specific for the lac transport system. The results show that APG2 inactivates from the outer surface of the vesicle membrane and support the previous hypothesis that the lac carrier protein is unable to bind external substrate in the absence of energy coupling."}
{"id": "PMID:1099096", "title": "Aspartate transcarbamylase of Escherichia coli. Mechanisms of inhibition and activation by dicarboxylic acids and other anions.", "content": "The interactions of several dicarboxylic acids and monoanions with Escherichia coli aspartate transcarbamylase and with its catalytic subunit have been studied by ultraviolet difference spectroscopy and steady state kinetics, with the following major findings. 1. A variety of dicarboxylic acids compete with carbamyl-P for the active sites of unliganded catalytic subunit, with steric requirements very different from those important for competition with L-aspartate for the subunit/carbamyl-P complex. Competition with carbamyl-P is much reduced if the dicarboxylic acid has a positively charged amino group. Acetate and chloride also compete. 2. At pH 7, equal concentrations of lysine acetate and L-aspartate are equally effective in displacing the transition state analog N-(phosphonacetyl)-L-aspartate (PALA) from the active sites of the concentrations of L-aspartate and lysine acetate is constant, increasing the concentration of L-aspartate does not relieve inhibition of the enzyme by PALA (Collins, K.C., and Stark, G. R. (1971) J. Biol. Chem. 246, 6599-6605). Therefore, the L-aspartate/subunit complex, like the acetate/subunit complex, must be incapable of participating in the catalytic reaction. We conclude that the kinetic mechanism is ordered, in agreement with the recent findings of Wedler and Gasser (Wedler, F.C., and Gasser, F.J. (1974), Arch. Biochem. Biophys. 163, 57-68) and in disagreement with the interpretation of Heyde et al. (Heyde, E., Nagabhushanam, A., And Morrison, J.F. (1973) Biochemistry 12, 4718-4726)...", "contents": "Aspartate transcarbamylase of Escherichia coli. Mechanisms of inhibition and activation by dicarboxylic acids and other anions. The interactions of several dicarboxylic acids and monoanions with Escherichia coli aspartate transcarbamylase and with its catalytic subunit have been studied by ultraviolet difference spectroscopy and steady state kinetics, with the following major findings. 1. A variety of dicarboxylic acids compete with carbamyl-P for the active sites of unliganded catalytic subunit, with steric requirements very different from those important for competition with L-aspartate for the subunit/carbamyl-P complex. Competition with carbamyl-P is much reduced if the dicarboxylic acid has a positively charged amino group. Acetate and chloride also compete. 2. At pH 7, equal concentrations of lysine acetate and L-aspartate are equally effective in displacing the transition state analog N-(phosphonacetyl)-L-aspartate (PALA) from the active sites of the concentrations of L-aspartate and lysine acetate is constant, increasing the concentration of L-aspartate does not relieve inhibition of the enzyme by PALA (Collins, K.C., and Stark, G. R. (1971) J. Biol. Chem. 246, 6599-6605). Therefore, the L-aspartate/subunit complex, like the acetate/subunit complex, must be incapable of participating in the catalytic reaction. We conclude that the kinetic mechanism is ordered, in agreement with the recent findings of Wedler and Gasser (Wedler, F.C., and Gasser, F.J. (1974), Arch. Biochem. Biophys. 163, 57-68) and in disagreement with the interpretation of Heyde et al. (Heyde, E., Nagabhushanam, A., And Morrison, J.F. (1973) Biochemistry 12, 4718-4726)..."}
{"id": "PMID:1099097", "title": "Binding of Met-tRNAf to native 40 S ribosomal subunits in Ehrlich ascites tumor cells.", "content": "Two forms of native 40 S ribosomal subunits, distinguishable by their buoyant densities, are recovered from Ehrlich ascites cells. In this communication, we describe experiments designed to test whether Met-tRNAf is associated with either form. Our results indicate that (a) in the cell, Met-tRNAf is bound to the native 40 S subunit, and in particular, to the subunit of density 1.40 g x cm-3; (b) under the growth conditions used, less than 10% of the low density native subunits have bound Met-tRNAf; (c) the majority of the Met-tRNAf containing native 40 S subunits, isolated from sucrose gradient analyses of cell extracts, join with 60 S subunits in vitro to form 80 S monosomes only if additional ribosomal wash factors are provided; and (d) the 80 S monosomes so formed are completed initiation complexes that can form peptide bonds. These results support the hypothesis that various forms of the native 40 S subunit represent different stages in the formation of a complete initiation complex.", "contents": "Binding of Met-tRNAf to native 40 S ribosomal subunits in Ehrlich ascites tumor cells. Two forms of native 40 S ribosomal subunits, distinguishable by their buoyant densities, are recovered from Ehrlich ascites cells. In this communication, we describe experiments designed to test whether Met-tRNAf is associated with either form. Our results indicate that (a) in the cell, Met-tRNAf is bound to the native 40 S subunit, and in particular, to the subunit of density 1.40 g x cm-3; (b) under the growth conditions used, less than 10% of the low density native subunits have bound Met-tRNAf; (c) the majority of the Met-tRNAf containing native 40 S subunits, isolated from sucrose gradient analyses of cell extracts, join with 60 S subunits in vitro to form 80 S monosomes only if additional ribosomal wash factors are provided; and (d) the 80 S monosomes so formed are completed initiation complexes that can form peptide bonds. These results support the hypothesis that various forms of the native 40 S subunit represent different stages in the formation of a complete initiation complex."}
{"id": "PMID:1099098", "title": "Evidence against a covalent intermediate in the adenosine triphosphate phosphoribosyltransferase reaction of histidine biosynthesis.", "content": "14C-Labeled 5-phospho-alpha-D-ribose-1-diphosphate (PRibPP) was synthesized and its interaction with adenosine triphosphate phosphoribosyltransferase was examined by gel filtration in a search for a form of this substrate covalently bound to the enzyme. Wide variation in solvent conditions gave little labeling of the enzyme. Heavy labeling was found only in the presence of the second substrate, ATP, and this was shown to arise from tightly but noncovalently bound product. Previous reports of a covalent intermediate in this enzymatic reaction probably were due to contaminating ATP in 5-phospho-alpha-D-ribose-1-diphosphate. Feedback inhibition of the enzyme by histidine was shown to occur at the step giving product or at some earlier step in the mechanism.", "contents": "Evidence against a covalent intermediate in the adenosine triphosphate phosphoribosyltransferase reaction of histidine biosynthesis. 14C-Labeled 5-phospho-alpha-D-ribose-1-diphosphate (PRibPP) was synthesized and its interaction with adenosine triphosphate phosphoribosyltransferase was examined by gel filtration in a search for a form of this substrate covalently bound to the enzyme. Wide variation in solvent conditions gave little labeling of the enzyme. Heavy labeling was found only in the presence of the second substrate, ATP, and this was shown to arise from tightly but noncovalently bound product. Previous reports of a covalent intermediate in this enzymatic reaction probably were due to contaminating ATP in 5-phospho-alpha-D-ribose-1-diphosphate. Feedback inhibition of the enzyme by histidine was shown to occur at the step giving product or at some earlier step in the mechanism."}
{"id": "PMID:1099099", "title": "Relation of growth and protein synthesis to the adenylate energy charge in an adenine-requiring mutant of Escherichia coli.", "content": "When Escherichia coli K-12 strain PC 0294, which is unable to synthesize adenine nucleotides, thereonine, proline, and leucine, was starved for adenine, the concentrations of ATP, ADP, and AMP fell rapidly. The adenylate energy charge was not affected until the adenine nucleotide pool fell to about 30% of its normal value. Similarly, cells in an adenine-limited chemostat grew at intracellular adenine nucleotide pool values as low as 30% of normal, but the energy charge in all cases was approximately 0.90. Incorporation of [14C]leucine into protein in adenine-starved cells continued rapidly as long as the energy charge was in or near its normal range, even when the concentration of ATP was 30% to 10% of its normal value. When glucose was added to cells that had been resuspended in medium lacking both glucose and adenine, the energy charge rose rapidly but the concentration of ATP fell, presumably because of nucleic acid synthesis. The rate of [14C]leucine incorporation into protein rose rapidly while adenine nucleotide concentrations fell, and then declined roughly in parallel with the energy charge. At a given value of energy and of total concentration of adenine nucleotides, the rate of protein synthesis may, of course, vary with the concentrations of precursors and modifiers; thus, the rate cannot be predicted from knowledge only of the energy charge. Our results suggest, however, that the rate of protein synthesis and the capacity for growth are much more sensitive to changes in the value of the energy charge than to changes in the concentration of ATP. Growth occurs when the ATP concentration is reduced to one-third of its normal value, but has not been observed when the energy charge has fallen by as much as 10%.", "contents": "Relation of growth and protein synthesis to the adenylate energy charge in an adenine-requiring mutant of Escherichia coli. When Escherichia coli K-12 strain PC 0294, which is unable to synthesize adenine nucleotides, thereonine, proline, and leucine, was starved for adenine, the concentrations of ATP, ADP, and AMP fell rapidly. The adenylate energy charge was not affected until the adenine nucleotide pool fell to about 30% of its normal value. Similarly, cells in an adenine-limited chemostat grew at intracellular adenine nucleotide pool values as low as 30% of normal, but the energy charge in all cases was approximately 0.90. Incorporation of [14C]leucine into protein in adenine-starved cells continued rapidly as long as the energy charge was in or near its normal range, even when the concentration of ATP was 30% to 10% of its normal value. When glucose was added to cells that had been resuspended in medium lacking both glucose and adenine, the energy charge rose rapidly but the concentration of ATP fell, presumably because of nucleic acid synthesis. The rate of [14C]leucine incorporation into protein rose rapidly while adenine nucleotide concentrations fell, and then declined roughly in parallel with the energy charge. At a given value of energy and of total concentration of adenine nucleotides, the rate of protein synthesis may, of course, vary with the concentrations of precursors and modifiers; thus, the rate cannot be predicted from knowledge only of the energy charge. Our results suggest, however, that the rate of protein synthesis and the capacity for growth are much more sensitive to changes in the value of the energy charge than to changes in the concentration of ATP. Growth occurs when the ATP concentration is reduced to one-third of its normal value, but has not been observed when the energy charge has fallen by as much as 10%."}
{"id": "PMID:1099100", "title": "\"Cytoplasmic\" deoxyribonucleic acid polymerase. Structure and properties of the highly purified enzyme from human KB cells.", "content": "The freshly prepared crude cytoplasmic fraction of aqueously extracted KB cells contains a single major species of DNA polymerase activity (DNA polymerase C) that sediments homogeneously in low ionic strength sucrose gradients with a peak at 10.8 S. The enzyme activity from frozen crude extracts sediments heterogeneously under these conditions with peaks at 8.4 and 10 S. In 0.45 M salt-containing gradients all of the polymerase activity is recovered as a single 6.4 S species. When purified to a specific activity of 7,300, DNA polymerase C sediments in low ionic strength gradients as a single species of 6.5 S. From combined sedimentation and gel filtration analysis, we estimate the molecular weight of the active protomeric species of the polymerase to be about 170,000. Under no conditions of ionic strength does the enzyme disaggregate to active species smaller than 6.4 to 6.5 S. Sodium dodecyl sulfate-polyacrylamide gel analysis of the most highly purified enzyme fractions reveals two major protein bands of 87,000 and 175,000 daltons, respectively. These data suggest that DNA polymerase C contains an 87,000-dalton component and permit the interpretation that the active protomer of Mr equal 170,000 may be a dimer. The purified enzyme shows maximal activity with gapped duplex DNA and has an absolute requirement for 3'-hydroxyl termini. It utilizes initiated polydeoxynucleotide templates poorly and initiated polyribonucleotide templates not at all. Although the polymerase is inhibited by PPi it has only minimal ability to promote PPi exchange (0.8% of the polymerase activity). The purified enzyme is free of endonuclease and exonuclease activities (less than or equal to 0.003% of the polymerase activity) and demonstrates no primer-template-dependent conversion of substrate dNTP to free dNMP during the polymerization reaction. Finally, DNA polymerase C does not excise misparied primer termini from a synthetic homopolymer primer-template but can utilize such termini as initiation sites, although at a very slow rate.", "contents": "\"Cytoplasmic\" deoxyribonucleic acid polymerase. Structure and properties of the highly purified enzyme from human KB cells. The freshly prepared crude cytoplasmic fraction of aqueously extracted KB cells contains a single major species of DNA polymerase activity (DNA polymerase C) that sediments homogeneously in low ionic strength sucrose gradients with a peak at 10.8 S. The enzyme activity from frozen crude extracts sediments heterogeneously under these conditions with peaks at 8.4 and 10 S. In 0.45 M salt-containing gradients all of the polymerase activity is recovered as a single 6.4 S species. When purified to a specific activity of 7,300, DNA polymerase C sediments in low ionic strength gradients as a single species of 6.5 S. From combined sedimentation and gel filtration analysis, we estimate the molecular weight of the active protomeric species of the polymerase to be about 170,000. Under no conditions of ionic strength does the enzyme disaggregate to active species smaller than 6.4 to 6.5 S. Sodium dodecyl sulfate-polyacrylamide gel analysis of the most highly purified enzyme fractions reveals two major protein bands of 87,000 and 175,000 daltons, respectively. These data suggest that DNA polymerase C contains an 87,000-dalton component and permit the interpretation that the active protomer of Mr equal 170,000 may be a dimer. The purified enzyme shows maximal activity with gapped duplex DNA and has an absolute requirement for 3'-hydroxyl termini. It utilizes initiated polydeoxynucleotide templates poorly and initiated polyribonucleotide templates not at all. Although the polymerase is inhibited by PPi it has only minimal ability to promote PPi exchange (0.8% of the polymerase activity). The purified enzyme is free of endonuclease and exonuclease activities (less than or equal to 0.003% of the polymerase activity) and demonstrates no primer-template-dependent conversion of substrate dNTP to free dNMP during the polymerization reaction. Finally, DNA polymerase C does not excise misparied primer termini from a synthetic homopolymer primer-template but can utilize such termini as initiation sites, although at a very slow rate."}
{"id": "PMID:1099101", "title": "Thermal regulation of the membrane lipid composition of Escherichia coli. Evidence for the direct control of fatty acid synthesis.", "content": "Starvation of strains of Escherichia coli which are glycerol auxotrophs and are also defective in beta oxidation results in the accumulation of large amounts of free fatty acid (Cronan, J. E., Jr., Weisberg, L. W., and Allen, R. G. (1975) J. Biol. Chem. 250, 5835-5840). We now report that the ratio of saturated to unsaturated species appearing in the free fatty acid fraction depends on the incubation temperature at the time of synthesis of these acids. This result indicates that fatty acid synthesis is one site of the thermal control of phospholipid fatty acid composition. We also report experiments on the incorporation of exogenously supplied fatty acids into membrane phospholipids. The effect of temperature on this incorporation supports the hypothesis that a second site of thermal regulation acts at the level of phosphatidic acid synthesis.", "contents": "Thermal regulation of the membrane lipid composition of Escherichia coli. Evidence for the direct control of fatty acid synthesis. Starvation of strains of Escherichia coli which are glycerol auxotrophs and are also defective in beta oxidation results in the accumulation of large amounts of free fatty acid (Cronan, J. E., Jr., Weisberg, L. W., and Allen, R. G. (1975) J. Biol. Chem. 250, 5835-5840). We now report that the ratio of saturated to unsaturated species appearing in the free fatty acid fraction depends on the incubation temperature at the time of synthesis of these acids. This result indicates that fatty acid synthesis is one site of the thermal control of phospholipid fatty acid composition. We also report experiments on the incorporation of exogenously supplied fatty acids into membrane phospholipids. The effect of temperature on this incorporation supports the hypothesis that a second site of thermal regulation acts at the level of phosphatidic acid synthesis."}
{"id": "PMID:1099103", "title": "Fractures and anatomical variations of the proximal portion of the fifth metatarsal.", "content": "Fractures of the tuberosity of the fifth metatarsal are frequent and with conservative treatment uniformly heal well. In contrast, fractures of the metatarsal shaft distal to the tuberosity heal slowly. Of 100 fractures of the tuberosity, almost all were clinically united within three weeks and roentgenographically united within two months. Of twenty fractures in the proximal part of the shaft, five required bone grafts and the time elapsed before roentgenographic and clinical evidence of union was variable and often prolonged.", "contents": "Fractures and anatomical variations of the proximal portion of the fifth metatarsal. Fractures of the tuberosity of the fifth metatarsal are frequent and with conservative treatment uniformly heal well. In contrast, fractures of the metatarsal shaft distal to the tuberosity heal slowly. Of 100 fractures of the tuberosity, almost all were clinically united within three weeks and roentgenographically united within two months. Of twenty fractures in the proximal part of the shaft, five required bone grafts and the time elapsed before roentgenographic and clinical evidence of union was variable and often prolonged."}
{"id": "PMID:1099104", "title": "Repair of experimental autologous grafts of cortical bone.", "content": "Fresh autologous segmental grafts of fibula, four centimeters long, were obtained from thirteen adult dogs. Holes were drilled in each before replantation. The amount of repair was determined after intervals of from four to twenty-four weeks. Torsional stress testing showed that the six drill holes 500 micrometers in diameter confined within an expanse of two centimeters did not mechanically weaken the transplants at the time of surgery. The drill holes were rapidly filled with cancellous bone which matured to cortical bone within twelve weeks. Microradiography and tetracycline labeling of drilled and non-drilled grafts demonstrated similar biological reparative patterns.", "contents": "Repair of experimental autologous grafts of cortical bone. Fresh autologous segmental grafts of fibula, four centimeters long, were obtained from thirteen adult dogs. Holes were drilled in each before replantation. The amount of repair was determined after intervals of from four to twenty-four weeks. Torsional stress testing showed that the six drill holes 500 micrometers in diameter confined within an expanse of two centimeters did not mechanically weaken the transplants at the time of surgery. The drill holes were rapidly filled with cancellous bone which matured to cortical bone within twelve weeks. Microradiography and tetracycline labeling of drilled and non-drilled grafts demonstrated similar biological reparative patterns."}
{"id": "PMID:1099105", "title": "Actin in erythrocyte ghosts and its association with spectrin.  Evidence for a nonfilamentous form of these two molecules in situ.", "content": "Actin was isolated from erythrocyte ghosts.  It is identical to muscle actin in its molecular weight, net charge, ability to polymerize into filaments with the double helical morphology, and its decoration with heavy meromyosin (HMM).  when erythrocyte ghosts are incubated in 0.1 mM EDTA, actin and spectrin are solubilized.  Spectrin has a larger molecular weight than muscle myosin.  When salt is added to the EDTA extract, a branching filamentous polymer is formed.  However, when muscle actin and the EDTA extract are mixed together in the presence of salt, the viscosity achieved is less than the viscosity of the solution if spectrin is omitted.  Thus, spectrin seems to inhibit the polymerization of actin.  If the actin is already polymerized, the addition of spectrin increases the viscosity of the solution, presumably by cross-linking the actin filaments.  The addition of HMM of trypsin to erythrocyte ghosts results in filament formation in situ.  These agents apparently act by detaching erythrocyte actin from spectrin, thereby allowing the polmerization of one or both proteins to occur.  Since filaments are not present in untreated erythrocyte ghosts, we conclude that erythrocyte actin and spectrin associate to form an anastomosing network beneath the erythrocyte membrane.  This network presumably functions in restricting the lateral movement of membrane-penetrating particles.", "contents": "Actin in erythrocyte ghosts and its association with spectrin.  Evidence for a nonfilamentous form of these two molecules in situ. Actin was isolated from erythrocyte ghosts.  It is identical to muscle actin in its molecular weight, net charge, ability to polymerize into filaments with the double helical morphology, and its decoration with heavy meromyosin (HMM).  when erythrocyte ghosts are incubated in 0.1 mM EDTA, actin and spectrin are solubilized.  Spectrin has a larger molecular weight than muscle myosin.  When salt is added to the EDTA extract, a branching filamentous polymer is formed.  However, when muscle actin and the EDTA extract are mixed together in the presence of salt, the viscosity achieved is less than the viscosity of the solution if spectrin is omitted.  Thus, spectrin seems to inhibit the polymerization of actin.  If the actin is already polymerized, the addition of spectrin increases the viscosity of the solution, presumably by cross-linking the actin filaments.  The addition of HMM of trypsin to erythrocyte ghosts results in filament formation in situ.  These agents apparently act by detaching erythrocyte actin from spectrin, thereby allowing the polmerization of one or both proteins to occur.  Since filaments are not present in untreated erythrocyte ghosts, we conclude that erythrocyte actin and spectrin associate to form an anastomosing network beneath the erythrocyte membrane.  This network presumably functions in restricting the lateral movement of membrane-penetrating particles."}
{"id": "PMID:1099106", "title": "Observations on the sorting-out of embryonic cells in monolayer culture.", "content": "Two problems are raised concerning the movement of cells during tissue-specific sorting-out of chick embryo cells in mixed aggregates. (i) A possible expectation from the hypothesis of 'contact inhibition' is that cells which are entirely surrounded by other cells in monolayer should be held stationary. Cells within solid aggregates, being totally surrounded by others, might also not be expected to move. How is it then that cell movement takes place within solid aggregates during sorting-out? (ii) Are the movements of cells within sorting aggregates 'passive', being driven by adhesive differentials or 'active', being merely guided by such differentials? In order to study these questions, sorting out experiments with chick embryonic limb bud mesenchyme and liver cells were carried out in monolayer culture, permitting direct observation of cell movements. Cell behavior was observed by time-lapse cinematography. Sorting-out of these cells in monolayer began before and continued after the cells had spread to confluency. During sorting, liver cells showed ruffing activity even when they appeared to be totally surrounded by other cells. Both cell types showed contact inhibition as judged by the criterion of monolayering, for they did not move over each other but remained attached to the substratum. Yet the cells in the confluent monolayer were not immobilized. Because of this, we suggest that the observed restraint against overlapping did not result from an inhibition of movement. Several considerations, detailed in the text, suggest that cell movement during sorting-out involve active locomotion. Previous work suggest that sorting-out configurations are determined by the relative intensities of intercellular adhesive strengths, the more cohesive of 2 cell populations tending to adopt the internal position. While limb bud cells form internal islands surrounded by liver cells in solid aggregates, the reverse was found to be the case in these monolayers. This suggests that, in the monolayer, limb bud cohesiveness is depressed relative to liver cell cohesiveness. This is consistent with the observation that the limb bud cells flattened themselves markedly against the substratum, significantly decreasing their area of mutual apposition.", "contents": "Observations on the sorting-out of embryonic cells in monolayer culture. Two problems are raised concerning the movement of cells during tissue-specific sorting-out of chick embryo cells in mixed aggregates. (i) A possible expectation from the hypothesis of 'contact inhibition' is that cells which are entirely surrounded by other cells in monolayer should be held stationary. Cells within solid aggregates, being totally surrounded by others, might also not be expected to move. How is it then that cell movement takes place within solid aggregates during sorting-out? (ii) Are the movements of cells within sorting aggregates 'passive', being driven by adhesive differentials or 'active', being merely guided by such differentials? In order to study these questions, sorting out experiments with chick embryonic limb bud mesenchyme and liver cells were carried out in monolayer culture, permitting direct observation of cell movements. Cell behavior was observed by time-lapse cinematography. Sorting-out of these cells in monolayer began before and continued after the cells had spread to confluency. During sorting, liver cells showed ruffing activity even when they appeared to be totally surrounded by other cells. Both cell types showed contact inhibition as judged by the criterion of monolayering, for they did not move over each other but remained attached to the substratum. Yet the cells in the confluent monolayer were not immobilized. Because of this, we suggest that the observed restraint against overlapping did not result from an inhibition of movement. Several considerations, detailed in the text, suggest that cell movement during sorting-out involve active locomotion. Previous work suggest that sorting-out configurations are determined by the relative intensities of intercellular adhesive strengths, the more cohesive of 2 cell populations tending to adopt the internal position. While limb bud cells form internal islands surrounded by liver cells in solid aggregates, the reverse was found to be the case in these monolayers. This suggests that, in the monolayer, limb bud cohesiveness is depressed relative to liver cell cohesiveness. This is consistent with the observation that the limb bud cells flattened themselves markedly against the substratum, significantly decreasing their area of mutual apposition."}
{"id": "PMID:1099107", "title": "Cell locomotion within a contact-inhibited monolayer of chick embryonic liver parenchyma cells.", "content": "Using time-lapse filming, the relative movement of cells (nuclei) within a contact-inhibited monolayer of chick embryonic liver parenchyma cells has been studied. Two techniques were employed to determine the amount of relative cell movement during a culture period of 6 h. Firstly, the number of neighbours lost or gained by each nucleus was counted. Secondly, the relative distance moved by each nucleus in relation to other nucleus in the monolayer was measured. (The numerical results obtained from these analyses and details of the methods used are given in the text). A considerable amount of relative movement of nuclei within the monolayer was found during this period of culture. Although some gaps were occasionally seen between the cells in the monolayer, it was observed that cells able both to \"ruffle\" and to translocate when no gap was detectable; i.e. the cells appeared able to move while entirely surrounded by other cells. Because of this, we suggest that the monolayering of these epitheloid cells on a surface may be due to restriction of overlapping between them rather than to inhibition of movement by mutual contact. We argue that the term \"contact inhibition of overlapping\" relates to this behaviour better than the term \"contact inhibition of movement\".", "contents": "Cell locomotion within a contact-inhibited monolayer of chick embryonic liver parenchyma cells. Using time-lapse filming, the relative movement of cells (nuclei) within a contact-inhibited monolayer of chick embryonic liver parenchyma cells has been studied. Two techniques were employed to determine the amount of relative cell movement during a culture period of 6 h. Firstly, the number of neighbours lost or gained by each nucleus was counted. Secondly, the relative distance moved by each nucleus in relation to other nucleus in the monolayer was measured. (The numerical results obtained from these analyses and details of the methods used are given in the text). A considerable amount of relative movement of nuclei within the monolayer was found during this period of culture. Although some gaps were occasionally seen between the cells in the monolayer, it was observed that cells able both to \"ruffle\" and to translocate when no gap was detectable; i.e. the cells appeared able to move while entirely surrounded by other cells. Because of this, we suggest that the monolayering of these epitheloid cells on a surface may be due to restriction of overlapping between them rather than to inhibition of movement by mutual contact. We argue that the term \"contact inhibition of overlapping\" relates to this behaviour better than the term \"contact inhibition of movement\"."}
{"id": "PMID:1099109", "title": "New methods of quantitation in thin-layer chromatography: tubular thin-layer chromatography [TTLC].", "content": "Techniques used in the quantitative evaluation of thin-layer chromatograms are compared. The quantitation of tubular thin-layer chromatograms by means of vapor-phase detectors is emphasized, and typical examples of the use of this method are outlined. Applications of tubular thin-layer chromatography (TTLC) in the quantitative analysis of fish liver oils are described.", "contents": "New methods of quantitation in thin-layer chromatography: tubular thin-layer chromatography [TTLC]. Techniques used in the quantitative evaluation of thin-layer chromatograms are compared. The quantitation of tubular thin-layer chromatograms by means of vapor-phase detectors is emphasized, and typical examples of the use of this method are outlined. Applications of tubular thin-layer chromatography (TTLC) in the quantitative analysis of fish liver oils are described."}
{"id": "PMID:1099111", "title": "A review of the analysis of free fatty acids [C2-C6].", "content": "A large number of the techniques and methods used to analyze FFA[C2-C6] from the advent of GLC to the present are reviewed. Possible reasons as to why it has taken such a long time to establish an unequivocal quantitative method are suggested. The results of analyzing standard solutions of FFA by three different methods are compared, and that based on the addition of formic acid to the carrier gas is recommended.", "contents": "A review of the analysis of free fatty acids [C2-C6]. A large number of the techniques and methods used to analyze FFA[C2-C6] from the advent of GLC to the present are reviewed. Possible reasons as to why it has taken such a long time to establish an unequivocal quantitative method are suggested. The results of analyzing standard solutions of FFA by three different methods are compared, and that based on the addition of formic acid to the carrier gas is recommended."}
{"id": "PMID:1099113", "title": "Response to LH-RH and clomiphene citrate in two women with the Prader-Labhart-Willi syndrome.", "content": "Two women with the Prader-Labhart-Willi syndrome are presented. The gonadotropin response to LH-RH administration was studied prior to, immediately following, and 6 months after a 6-week trial of clomiphene citrate, 200 mg per day for 21 days in divided doses, followed by 100 mg for  21 days in divided doses, followed by 100 mg per day for 14 days in divided doses, and followed by 50 mg per day in a single dose for an additional 14 days. During therapy, the basal gonadotropin and estradiol concentrations rose from prepubertal levels to those of mature women in midmenstrual cycle. However, 6 months after cessation of treatment, the basal gondadotropin and estradiol levels had returned to the prepubertal range. The initial response to LH-RH in the 2 patients differed in that one was clearly prepubertal and the other indistinguishable from the broad range of the adult normal response. The LY and FSH responses to LH-RH administration was greater after 6 weeks of clomiphene citrate therapy than they were either before (both patients) or 6 months after treatment (1 patient). We conclude that there is heterogeneity in the response to LH-RH administration in the Prader-Labhart-Willi Syndrome, just as there is in other syndromes of hypogonadotropic hypogonadism. A normal adult response of gonadotropins to the administration of LH-RH was acheived during clomiphene citrate therapy.", "contents": "Response to LH-RH and clomiphene citrate in two women with the Prader-Labhart-Willi syndrome. Two women with the Prader-Labhart-Willi syndrome are presented. The gonadotropin response to LH-RH administration was studied prior to, immediately following, and 6 months after a 6-week trial of clomiphene citrate, 200 mg per day for 21 days in divided doses, followed by 100 mg for  21 days in divided doses, followed by 100 mg per day for 14 days in divided doses, and followed by 50 mg per day in a single dose for an additional 14 days. During therapy, the basal gonadotropin and estradiol concentrations rose from prepubertal levels to those of mature women in midmenstrual cycle. However, 6 months after cessation of treatment, the basal gondadotropin and estradiol levels had returned to the prepubertal range. The initial response to LH-RH in the 2 patients differed in that one was clearly prepubertal and the other indistinguishable from the broad range of the adult normal response. The LY and FSH responses to LH-RH administration was greater after 6 weeks of clomiphene citrate therapy than they were either before (both patients) or 6 months after treatment (1 patient). We conclude that there is heterogeneity in the response to LH-RH administration in the Prader-Labhart-Willi Syndrome, just as there is in other syndromes of hypogonadotropic hypogonadism. A normal adult response of gonadotropins to the administration of LH-RH was acheived during clomiphene citrate therapy."}
{"id": "PMID:1099114", "title": "A computer program that evaluates patients with hypercalcemia.", "content": "A computer program has been developed to assist the physician in managing patients with hypercalcemia. The program requests appropriate information, such as the patient's serum calcium concentration, age and x-ray results. Based on the abnormalities detected, the program asks additional questions needed to suggest the most helpful course of action for evaluating or treating the patient's hypercalcemia. As soon as the data have been entered, the program generates an evaluation note that includes diagnostic possibilities, suggestions for additional laboratory studies, therapeutic recommendations and references to the medical literature. The program can be used from any general purpose teletype-compatible terminal (including cathode ray tube displays) connected to the Bell Telephone System. The time required to enter the data and obtain the evaluation note is approximately 4 minutes with a teletypewriter, and less if a faster terminal is used. The cost for computer time is comparable to that of a laboratory test. Comparison of diagnoses produced by the program with those obtained from pathologic examination of from consulting endocrinolgists shows that the program is sufficiently accurate for clinical use. Finnally, the explanation of the pathophysiology contained in the evaluation note may be useful to the student.", "contents": "A computer program that evaluates patients with hypercalcemia. A computer program has been developed to assist the physician in managing patients with hypercalcemia. The program requests appropriate information, such as the patient's serum calcium concentration, age and x-ray results. Based on the abnormalities detected, the program asks additional questions needed to suggest the most helpful course of action for evaluating or treating the patient's hypercalcemia. As soon as the data have been entered, the program generates an evaluation note that includes diagnostic possibilities, suggestions for additional laboratory studies, therapeutic recommendations and references to the medical literature. The program can be used from any general purpose teletype-compatible terminal (including cathode ray tube displays) connected to the Bell Telephone System. The time required to enter the data and obtain the evaluation note is approximately 4 minutes with a teletypewriter, and less if a faster terminal is used. The cost for computer time is comparable to that of a laboratory test. Comparison of diagnoses produced by the program with those obtained from pathologic examination of from consulting endocrinolgists shows that the program is sufficiently accurate for clinical use. Finnally, the explanation of the pathophysiology contained in the evaluation note may be useful to the student."}
{"id": "PMID:1099115", "title": "Secretion of alpha subunits of luteinizing hormone (LH) by the anterior pituitary.", "content": "Free alpha subunit chains of the glycopeptide pituitary hormones have been found in the sera of normal subjects and postmenopausal women. To ascertain whether the alpha subunit of LH is directly secreted by the pituitary or formed as a result of degradation of intact LY in the periphery, alpha subunits and intact LH were measured by radioimmunoassay in human volunteers after LRF stimulation and purified LH infusion. In 4 subjects a loading dose of 90 IU, followed by the infusion of 22.5 IJ of purified human LH over 30 min, produced peak serum LH levels of 41 mIU/ml but no change in alpha subunit levels of 35 IU of purified human LH to an additional 4 subjects, produced peak LH levels of 8* mIU/ml, but again, no change in alpha subunits. In the same two groups of subjects 100 mug of LRF produced peak LH levels of 25 mIU/ml and 75 mIU/ml, respectively, with significant alpha subunit elevations at 20 min of 1.7 ng/ml and 2.7 ng/ml, respectively. In separate groups of men LRF was administered over a wide dose range of 1 to 3,000 mug and LH and the alpha subunit measured. A dose-response curve existed over the entire LRF dose range for blood LH; no minimum or maximum plateaus were observed over the range studied. However, the alpha chain response appeared to reach a maximal plateau at a dose of 100 mug of LRF. The results are compativle with the hypothesis that the alpha subunits appearing in the peripheral circulation in response to LRF are due to secretion by the anterior pituitary and not due to peripheral degradation of intact secreted LH.", "contents": "Secretion of alpha subunits of luteinizing hormone (LH) by the anterior pituitary. Free alpha subunit chains of the glycopeptide pituitary hormones have been found in the sera of normal subjects and postmenopausal women. To ascertain whether the alpha subunit of LH is directly secreted by the pituitary or formed as a result of degradation of intact LY in the periphery, alpha subunits and intact LH were measured by radioimmunoassay in human volunteers after LRF stimulation and purified LH infusion. In 4 subjects a loading dose of 90 IU, followed by the infusion of 22.5 IJ of purified human LH over 30 min, produced peak serum LH levels of 41 mIU/ml but no change in alpha subunit levels of 35 IU of purified human LH to an additional 4 subjects, produced peak LH levels of 8* mIU/ml, but again, no change in alpha subunits. In the same two groups of subjects 100 mug of LRF produced peak LH levels of 25 mIU/ml and 75 mIU/ml, respectively, with significant alpha subunit elevations at 20 min of 1.7 ng/ml and 2.7 ng/ml, respectively. In separate groups of men LRF was administered over a wide dose range of 1 to 3,000 mug and LH and the alpha subunit measured. A dose-response curve existed over the entire LRF dose range for blood LH; no minimum or maximum plateaus were observed over the range studied. However, the alpha chain response appeared to reach a maximal plateau at a dose of 100 mug of LRF. The results are compativle with the hypothesis that the alpha subunits appearing in the peripheral circulation in response to LRF are due to secretion by the anterior pituitary and not due to peripheral degradation of intact secreted LH."}
{"id": "PMID:1099116", "title": "Secondary drug failure occurring during chronic treatment with LHRH: appearance of an antibody.", "content": "Secondary drug failure with regard to subjective and hormonal responses occurred in a patient treated chronically with LHRH. This failure of response to hormone therapy was correlated with increased binding of 125I-LHRH by the patient's sera. Also, competitive displacement of 125I-LHRH by unlabelled LHRH was demonstrated. Affinity constants and titers of order appropriate for antibody were obtained. These data support strongly a report of antibody formation to exogenous LHRH in man.", "contents": "Secondary drug failure occurring during chronic treatment with LHRH: appearance of an antibody. Secondary drug failure with regard to subjective and hormonal responses occurred in a patient treated chronically with LHRH. This failure of response to hormone therapy was correlated with increased binding of 125I-LHRH by the patient's sera. Also, competitive displacement of 125I-LHRH by unlabelled LHRH was demonstrated. Affinity constants and titers of order appropriate for antibody were obtained. These data support strongly a report of antibody formation to exogenous LHRH in man."}
{"id": "PMID:1099117", "title": "Circulating polyribophosphate in Hemophilus influenzae, type b meningitis. Correlation with clinical course and antibody response.", "content": "In systemic infections caused by Hemophilus influenzae, type b, the capsular polysaccharide, polyribophosphate, is released into the circulation. Polyribophosphate was quantitated in serial serum and cerebrospinal fluid samples from 45 children with H. influenzae, type b meningitis by means of a radiolabeled antigen-binding inhibition assay. Polyribophosphate was regularly found in acute serum and cerebrospinal fluid samples and could be detected in unbound form for periods of 1-30 days after initiation of effective therapy. Complexes of polyribophosphate dissociable with acid and pepsin were detected in serum samples from 17 patients, in one case for a period of 145 days after hospitalization. Polyribophosphate levels and patterns of clearance were studied in relation to hospital course and antibody response. Patients with prolonged antigenemia had protracted fevers and severe neurological symptoms during hospitalization, frequently with focal complications.Antipolyribophosphate antibody responses were detected during the first 100 days of convalescence by radioimmunoassay in 79% of the patients studied, including 60% of the children 1 yr or less in age. The intensity of antibody response although clearly related to the age of the patient, was more reliably predicted by the efficiency of antigen clearance. Antibody responses were uniformly of low magnitude in patients with prolonged antigenemia, irrespective of age. Paients who failed to develop antibody to polyribophosphate after meningitis also exhibited impaired antigen clearance. These studies suggest that mechanisms necessary for clearance of polyribophosphate may influence the development and intensity of the humoral immune response and raise the possibility of developmental deficiencies in the clearance system in infants and children.", "contents": "Circulating polyribophosphate in Hemophilus influenzae, type b meningitis. Correlation with clinical course and antibody response. In systemic infections caused by Hemophilus influenzae, type b, the capsular polysaccharide, polyribophosphate, is released into the circulation. Polyribophosphate was quantitated in serial serum and cerebrospinal fluid samples from 45 children with H. influenzae, type b meningitis by means of a radiolabeled antigen-binding inhibition assay. Polyribophosphate was regularly found in acute serum and cerebrospinal fluid samples and could be detected in unbound form for periods of 1-30 days after initiation of effective therapy. Complexes of polyribophosphate dissociable with acid and pepsin were detected in serum samples from 17 patients, in one case for a period of 145 days after hospitalization. Polyribophosphate levels and patterns of clearance were studied in relation to hospital course and antibody response. Patients with prolonged antigenemia had protracted fevers and severe neurological symptoms during hospitalization, frequently with focal complications.Antipolyribophosphate antibody responses were detected during the first 100 days of convalescence by radioimmunoassay in 79% of the patients studied, including 60% of the children 1 yr or less in age. The intensity of antibody response although clearly related to the age of the patient, was more reliably predicted by the efficiency of antigen clearance. Antibody responses were uniformly of low magnitude in patients with prolonged antigenemia, irrespective of age. Paients who failed to develop antibody to polyribophosphate after meningitis also exhibited impaired antigen clearance. These studies suggest that mechanisms necessary for clearance of polyribophosphate may influence the development and intensity of the humoral immune response and raise the possibility of developmental deficiencies in the clearance system in infants and children."}
{"id": "PMID:1099118", "title": "A morphological basis for intercellular communication between alpha- and beta-cells in the endocrine pancreas.", "content": "By degranulating beta-cells in the islets of Langerhans of the rat with sulfonylurea, it has been possible to distinguish unambiguously alpha-cells from beta-cells in freeze-fracture replicas. In such preparations, we found morphologically typical tight and gap junctions occurring between alpha- and beta-cells. The presence of gap junctions offers indirect evidence that these cells are coupled with one another; coupling may influence the secretory behavior of alpha- and beta-cells maintaining glucose homeostasis within tightly constricted limits.", "contents": "A morphological basis for intercellular communication between alpha- and beta-cells in the endocrine pancreas. By degranulating beta-cells in the islets of Langerhans of the rat with sulfonylurea, it has been possible to distinguish unambiguously alpha-cells from beta-cells in freeze-fracture replicas. In such preparations, we found morphologically typical tight and gap junctions occurring between alpha- and beta-cells. The presence of gap junctions offers indirect evidence that these cells are coupled with one another; coupling may influence the secretory behavior of alpha- and beta-cells maintaining glucose homeostasis within tightly constricted limits."}
{"id": "PMID:1099119", "title": "Functional role of antibody against \"core\" glycolipid of Enterobacteriaceae.", "content": "Antibodies against the \"core\" glycolipid of Enterobacteriaceae (2-keto, 3-deoxyoctonate-Lipid A) have been associated with protection against the sequelae of gram-negative rod bacteremia. To investigate the nature of this protection, dogs and rabbits were immunized with purified glycolipid prepared by phenol-chloroform-petroleum ether extraction of the \"Re\" mutant of Salmonella minnesota 595 and opsonophagocytic and bactericidal tests were carried out using lapine peritoneal granulocytes and serum factors. Whereas 1-4 mug/kg of glycolipid i.v. produced hypotension in dogs and 8 mug/kg i.v. was lethal, a rising dosage schedule of immunization with an average total dose of 1 mg/kg produced striking protection against shock and death against challenge with heterologous organisms. 20 control dogs, given approximately 10(10) live, serum-resistant Escherichia coli 0.85:H9 or Serratia marcescens 03 during a continuous intra-arterial pressure transducer recording, showed a drop in mean systolic pressure from 186 (+/- 6 SE) to 101 (+/- 12 SE) MM Hg and a fall in mean diastolic pressure from 118 (+/- 3 SE) to 64 (+/- 8 SE) mm Hg within 60-120 min. Minor pressure changes (average less than 12% of prechallenge levels) were seen in the same number of immunized dogs. In contrast, no significant difference was noted in the bloodstream clearance of these serum-resistant organisms over a period of 4-6 h between immunized and control dogs. Intravascular clearance was greater in animals immunized with the challenged strain or in glycolipid-immunized animals challenged with highly serum-sensitive E. coli 0.14:K7. Antibody against core glycolipid protected against the hemodynamic sequelae of bacillemia, augmented intravascular clearance of serum-sensitive organisms, and abrogated the pyrogenic response to enteric bacilli, but did not enhance clearance of serum-resistant organisms. Although canine and lapine antiserum against core glycolipid passively protected mice against a heterologous challenge, opsonophagocytic and bactericidal activity was at least 100-fold less than type-specific antiserum.", "contents": "Functional role of antibody against \"core\" glycolipid of Enterobacteriaceae. Antibodies against the \"core\" glycolipid of Enterobacteriaceae (2-keto, 3-deoxyoctonate-Lipid A) have been associated with protection against the sequelae of gram-negative rod bacteremia. To investigate the nature of this protection, dogs and rabbits were immunized with purified glycolipid prepared by phenol-chloroform-petroleum ether extraction of the \"Re\" mutant of Salmonella minnesota 595 and opsonophagocytic and bactericidal tests were carried out using lapine peritoneal granulocytes and serum factors. Whereas 1-4 mug/kg of glycolipid i.v. produced hypotension in dogs and 8 mug/kg i.v. was lethal, a rising dosage schedule of immunization with an average total dose of 1 mg/kg produced striking protection against shock and death against challenge with heterologous organisms. 20 control dogs, given approximately 10(10) live, serum-resistant Escherichia coli 0.85:H9 or Serratia marcescens 03 during a continuous intra-arterial pressure transducer recording, showed a drop in mean systolic pressure from 186 (+/- 6 SE) to 101 (+/- 12 SE) MM Hg and a fall in mean diastolic pressure from 118 (+/- 3 SE) to 64 (+/- 8 SE) mm Hg within 60-120 min. Minor pressure changes (average less than 12% of prechallenge levels) were seen in the same number of immunized dogs. In contrast, no significant difference was noted in the bloodstream clearance of these serum-resistant organisms over a period of 4-6 h between immunized and control dogs. Intravascular clearance was greater in animals immunized with the challenged strain or in glycolipid-immunized animals challenged with highly serum-sensitive E. coli 0.14:K7. Antibody against core glycolipid protected against the hemodynamic sequelae of bacillemia, augmented intravascular clearance of serum-sensitive organisms, and abrogated the pyrogenic response to enteric bacilli, but did not enhance clearance of serum-resistant organisms. Although canine and lapine antiserum against core glycolipid passively protected mice against a heterologous challenge, opsonophagocytic and bactericidal activity was at least 100-fold less than type-specific antiserum."}
{"id": "PMID:1099120", "title": "Behavior of eosinophil leukocytes in acute inflammation. II. Eosinophil dynamics during acute inflammation.", "content": "The marked diminution in the number of circulating eosinophils, which has been shown to occur during acute bacterial infections, is a distinctive aspect of eosinophil physiology and of the host response to acute infection. The mouse rendered eosinophilic by infection with trichinosis provides a suitable model for study of the eosinopenic response induced by acute inflammation. The alterations in eosinophil dynamics associated with acute inflammatory reactions in trichinous mice were studied with pneumococcal abscesses, with Escherichia coli pyelonephritis, with Coxsackie viral pancreatitis, and with acute subcutaneous inflammation due to turpentine. Each of these stimuli of acute inflammation markedly suppressed the eosinophilia of trichinosis. This suggests that the eosinopenia is a response to the acute inflammatory process rather than the response to a specific type of pathogen. These studies apply quantitative techniques to ascertain the effects of acute inflammation on eosinophil production in bone marrow and on distribution of eosinophils in the peripheral tissues. From these observations, it is apparent that the initial response to acute inflammation includes a rapid drop in numbers of circulating eosinophils, a rapid accumulation of eosinophils at the periphery of the inflammatory site, and an inhibition of egress of eosinophils from the bone marrow. With prolongation of the inflammatory process, inhibition of eosinopoiesis occurs.", "contents": "Behavior of eosinophil leukocytes in acute inflammation. II. Eosinophil dynamics during acute inflammation. The marked diminution in the number of circulating eosinophils, which has been shown to occur during acute bacterial infections, is a distinctive aspect of eosinophil physiology and of the host response to acute infection. The mouse rendered eosinophilic by infection with trichinosis provides a suitable model for study of the eosinopenic response induced by acute inflammation. The alterations in eosinophil dynamics associated with acute inflammatory reactions in trichinous mice were studied with pneumococcal abscesses, with Escherichia coli pyelonephritis, with Coxsackie viral pancreatitis, and with acute subcutaneous inflammation due to turpentine. Each of these stimuli of acute inflammation markedly suppressed the eosinophilia of trichinosis. This suggests that the eosinopenia is a response to the acute inflammatory process rather than the response to a specific type of pathogen. These studies apply quantitative techniques to ascertain the effects of acute inflammation on eosinophil production in bone marrow and on distribution of eosinophils in the peripheral tissues. From these observations, it is apparent that the initial response to acute inflammation includes a rapid drop in numbers of circulating eosinophils, a rapid accumulation of eosinophils at the periphery of the inflammatory site, and an inhibition of egress of eosinophils from the bone marrow. With prolongation of the inflammatory process, inhibition of eosinopoiesis occurs."}
{"id": "PMID:1099121", "title": "Hereditary deficiency of the seventh component of complement.", "content": "Deficiency of the seventh component of complement has been found in the serum of a 42-yr-old Caucasian woman who has Raynaud's phenomenon, sclerodactyly, and telangiectasia. Partial deficiency was found in the serum of the patient's parents and children, indicating a pattern of inheritance of autosomal codominance. Transfusion experiments indicated that exogenous C7 had a 91-h halk-life in the patient. There was no evidence for C7 synthesis after transfusion. No C7 inhibitors were detected in the patient's serum. The patient's serum was found to support the activation of complement by both the classical and properdin pathways to the C7 stage. The addition of C7 to the patient's serum permitted it to support hemolytic reactions initiated by either pathway. No defects could be detected in plasma or whole blood coagulation. The patient's serum was deficient in opsonizing unsensitized yeast particles in serum and in the generation of chemotactic factor by antigen-antibody complexes and endotoxin. Both deficiencies were corrected by the addition of C7. These observations suggest a key role for C7 for in vitro yeast phagocytosis and chemotaxis generation. However, the patient's lack of infections indicates a relatively minor role for C7 in human resistance to infection.", "contents": "Hereditary deficiency of the seventh component of complement. Deficiency of the seventh component of complement has been found in the serum of a 42-yr-old Caucasian woman who has Raynaud's phenomenon, sclerodactyly, and telangiectasia. Partial deficiency was found in the serum of the patient's parents and children, indicating a pattern of inheritance of autosomal codominance. Transfusion experiments indicated that exogenous C7 had a 91-h halk-life in the patient. There was no evidence for C7 synthesis after transfusion. No C7 inhibitors were detected in the patient's serum. The patient's serum was found to support the activation of complement by both the classical and properdin pathways to the C7 stage. The addition of C7 to the patient's serum permitted it to support hemolytic reactions initiated by either pathway. No defects could be detected in plasma or whole blood coagulation. The patient's serum was deficient in opsonizing unsensitized yeast particles in serum and in the generation of chemotactic factor by antigen-antibody complexes and endotoxin. Both deficiencies were corrected by the addition of C7. These observations suggest a key role for C7 for in vitro yeast phagocytosis and chemotaxis generation. However, the patient's lack of infections indicates a relatively minor role for C7 in human resistance to infection."}
{"id": "PMID:1099122", "title": "An animal model of hypersensitivity pneumonitis in the rabbit.", "content": "This study was devised to produce an animal model of hypersensitivity pneumonitis in order to study both the induction and the elicitation of the disease. Rabbits exposed by aerosol to large quantities of pigeon antigens developed a humoral, but not cellular, immunologic response. Moreover, their lungs were essentially normal histologically. A single i.v. injection of killed BCG in oil permitted the induction of pulmonary cell-medid hypersensitivity to the inhaled antigen, as well as the development of pulmonary lesions which were more severe than that caused by the administration of BCG alone. The humoral immunologic response to the inhaled antigen was not increased after BCG injection. Since many individuals are exposed to the etiologic agents of hypersensitivity pneumonitis for extended periods without developing the disease, these findings in animals suggest that some event may occur to induce cell mediated hypersensitivity in order to initiate the disease process. In addition, we have shown that animals with normal lung histology and circulating complement-fixing antibodies undergo serum complement (CH50) depression after an aerosol challenge with the specific antigen. Animals with circulating, complement-fixing antibodies, and inflamed lungs (BCG-induced failed to undergo a complement depression subsequent to an aerosol challenge with specific antigens. These results re consistent with those seen in symptomatic and asymptomatic pigeon breeders and suggest that antigen distribution through the lung is important in the pathogenesis of hypersensitivity pneumonitis.", "contents": "An animal model of hypersensitivity pneumonitis in the rabbit. This study was devised to produce an animal model of hypersensitivity pneumonitis in order to study both the induction and the elicitation of the disease. Rabbits exposed by aerosol to large quantities of pigeon antigens developed a humoral, but not cellular, immunologic response. Moreover, their lungs were essentially normal histologically. A single i.v. injection of killed BCG in oil permitted the induction of pulmonary cell-medid hypersensitivity to the inhaled antigen, as well as the development of pulmonary lesions which were more severe than that caused by the administration of BCG alone. The humoral immunologic response to the inhaled antigen was not increased after BCG injection. Since many individuals are exposed to the etiologic agents of hypersensitivity pneumonitis for extended periods without developing the disease, these findings in animals suggest that some event may occur to induce cell mediated hypersensitivity in order to initiate the disease process. In addition, we have shown that animals with normal lung histology and circulating complement-fixing antibodies undergo serum complement (CH50) depression after an aerosol challenge with the specific antigen. Animals with circulating, complement-fixing antibodies, and inflamed lungs (BCG-induced failed to undergo a complement depression subsequent to an aerosol challenge with specific antigens. These results re consistent with those seen in symptomatic and asymptomatic pigeon breeders and suggest that antigen distribution through the lung is important in the pathogenesis of hypersensitivity pneumonitis."}
{"id": "PMID:1099124", "title": "Lactational responses to postruminal administration of proteins and amino acids.", "content": "Rumen by-pass of nutrients has improved performance of lactating dairy cows, growing stepers, and sheep. Postruminal supplementation of casein has increased milk production 1 to 4 kg per cow per day and milk protein yield by 10 to 15%. Component(s) in casein eliciting the improved performance have not been determined adequately. Glucose, amino acids, hormone secretion, or a combination of these factors may be responsible for the improved performance. Future research to delineate limiting nutrients for postruminal supplementation should prove fruitful and should make a major contribution to improved production by ruminant animals.", "contents": "Lactational responses to postruminal administration of proteins and amino acids. Rumen by-pass of nutrients has improved performance of lactating dairy cows, growing stepers, and sheep. Postruminal supplementation of casein has increased milk production 1 to 4 kg per cow per day and milk protein yield by 10 to 15%. Component(s) in casein eliciting the improved performance have not been determined adequately. Glucose, amino acids, hormone secretion, or a combination of these factors may be responsible for the improved performance. Future research to delineate limiting nutrients for postruminal supplementation should prove fruitful and should make a major contribution to improved production by ruminant animals."}
{"id": "PMID:1099125", "title": "Rumen bypass and protection of proteins and amino acids.", "content": "Potent rumen microbial proteases and deaminases rapidly degrade protein and amino acids which are soluble in the rumen liquid phase. Because protein sources vary in their solubility, the degree of degradation in the rumen is variable. Methods of decreasing protein and amino acid degradation in the rumen include heat treatment, chemical treatment, encapsulation, use of amino acid analogs, selective manipulation of balances of rumen metabolic pathways, and esophageal groove closure. It is important that procedures do not interfere with ruminal metabolism or post-ruminal digestion. Bypassing the rumen changes sites in the digestive tract of nutrient digestion and absorption and provides a mechanism for supplementing outflow of nutrients from the rumen. A feasible approach to production of animal protein from ruminants would be utilization of nonprotein nitrogen for rumen protein production, maximization of rumen bypass of dietary protein, and supplementation with rumen nondegradable amino acids.", "contents": "Rumen bypass and protection of proteins and amino acids. Potent rumen microbial proteases and deaminases rapidly degrade protein and amino acids which are soluble in the rumen liquid phase. Because protein sources vary in their solubility, the degree of degradation in the rumen is variable. Methods of decreasing protein and amino acid degradation in the rumen include heat treatment, chemical treatment, encapsulation, use of amino acid analogs, selective manipulation of balances of rumen metabolic pathways, and esophageal groove closure. It is important that procedures do not interfere with ruminal metabolism or post-ruminal digestion. Bypassing the rumen changes sites in the digestive tract of nutrient digestion and absorption and provides a mechanism for supplementing outflow of nutrients from the rumen. A feasible approach to production of animal protein from ruminants would be utilization of nonprotein nitrogen for rumen protein production, maximization of rumen bypass of dietary protein, and supplementation with rumen nondegradable amino acids."}
{"id": "PMID:1099126", "title": "Nitrogen requirement and utilization in dairy cattle.", "content": "Formulation of dairy cow rations should consider the following points regarding nitrogen utilization by lactating cows. (a) Maintenance of ruminal ammonia nitrogen in excess of 5 mg/100 ml rumen fluid has no effect on microbial protein production. (b)Supplemental nonprotein nitrogen is not utilized in typical dairy and feedlot beef rations containing more than 12 to 13% crude protein(dry matter basis). (c)Nonprotein nitrogen is approximately equal to true protein as a source of nitrogen in typical dairy and feedlot rations containing not more than 12 to 13% crude protein. (d)A scheme based upon metabolizable protein (absorbable protein) for calculating requirements and comparing protein sources is superior to crude or digestible protein designations. Ultimate expression of the requirement may be in terms of crude protein for the sake of simplicity. (e)One kilogram of crude protein, regardless of nitrogen source, equals about .75kg metabolizable protein in typical dairy and feedlot beef rations containing not more than 12 to 13% crude protein. One kilogram of plant protein (true protein) fed in excess of an amount equivalent to 12 to 13% dietary protein equals about .3 kg metabolizable protein. (f)Protein supplementation of lactating cows might be related more to stage of lactation than to milk production. (g)Lactating cows having above average lactational ability may benefit from dietary protein as high as 16 to 17% (dry matter basis) during the first third of lactation. (h)Cows in the latter two-thirds of lactation appear to require 12.5% dietary protein or less. (i)Plant protein (true protein) should be the supplemental sources of nitrogen during the first third of lactation, with NPN providing most, if not all, the supplemental nitrogen during the last two-thirds of lactation.", "contents": "Nitrogen requirement and utilization in dairy cattle. Formulation of dairy cow rations should consider the following points regarding nitrogen utilization by lactating cows. (a) Maintenance of ruminal ammonia nitrogen in excess of 5 mg/100 ml rumen fluid has no effect on microbial protein production. (b)Supplemental nonprotein nitrogen is not utilized in typical dairy and feedlot beef rations containing more than 12 to 13% crude protein(dry matter basis). (c)Nonprotein nitrogen is approximately equal to true protein as a source of nitrogen in typical dairy and feedlot rations containing not more than 12 to 13% crude protein. (d)A scheme based upon metabolizable protein (absorbable protein) for calculating requirements and comparing protein sources is superior to crude or digestible protein designations. Ultimate expression of the requirement may be in terms of crude protein for the sake of simplicity. (e)One kilogram of crude protein, regardless of nitrogen source, equals about .75kg metabolizable protein in typical dairy and feedlot beef rations containing not more than 12 to 13% crude protein. One kilogram of plant protein (true protein) fed in excess of an amount equivalent to 12 to 13% dietary protein equals about .3 kg metabolizable protein. (f)Protein supplementation of lactating cows might be related more to stage of lactation than to milk production. (g)Lactating cows having above average lactational ability may benefit from dietary protein as high as 16 to 17% (dry matter basis) during the first third of lactation. (h)Cows in the latter two-thirds of lactation appear to require 12.5% dietary protein or less. (i)Plant protein (true protein) should be the supplemental sources of nitrogen during the first third of lactation, with NPN providing most, if not all, the supplemental nitrogen during the last two-thirds of lactation."}
{"id": "PMID:1099132", "title": "Juvenile hysteria and primary shock: report of case.", "content": "Hysterical aberrations may occur from circumstances other than fear and anxiety in a dental office. Irreversible shock from hysteria is well documented. It is important that causative factors of hysterical experiences be properly and promptly identified, and adequate measures be taken to allay these factors so that permanent adverse effects of the hysteria are aborted.", "contents": "Juvenile hysteria and primary shock: report of case. Hysterical aberrations may occur from circumstances other than fear and anxiety in a dental office. Irreversible shock from hysteria is well documented. It is important that causative factors of hysterical experiences be properly and promptly identified, and adequate measures be taken to allay these factors so that permanent adverse effects of the hysteria are aborted."}
{"id": "PMID:1099142", "title": "Surveying.", "content": "Several systems for the survey and design for removable partial dentures are based on empiricism and misinformation. Presented here is a systematic approach to the basic requirements for precribing a prosthesis that will be received favorably by the patient.", "contents": "Surveying. Several systems for the survey and design for removable partial dentures are based on empiricism and misinformation. Presented here is a systematic approach to the basic requirements for precribing a prosthesis that will be received favorably by the patient."}
{"id": "PMID:1099137", "title": "Effect of humidity and temperature changes on orthodontic direct-bonding adhesive systems.", "content": "Plastic brackets were bonded to 560 extracted human teeth with use of two orthodontic adhesive systems: (1) methyl methacrylate resin bonded to a sealant that was polymerized using ultraviolet light, and (2) self-polymerizing methyl methacrylate resin bonded directly to the etched enamel. Tensile and shearlike tests were performed. Prolonged exposure to heat, moisture, and severe temperature changes decreased the shearlike strength of both adhesives. Both systems were adequately strong to withstand routine orthodontic and estimated masticatory forces.", "contents": "Effect of humidity and temperature changes on orthodontic direct-bonding adhesive systems. Plastic brackets were bonded to 560 extracted human teeth with use of two orthodontic adhesive systems: (1) methyl methacrylate resin bonded to a sealant that was polymerized using ultraviolet light, and (2) self-polymerizing methyl methacrylate resin bonded directly to the etched enamel. Tensile and shearlike tests were performed. Prolonged exposure to heat, moisture, and severe temperature changes decreased the shearlike strength of both adhesives. Both systems were adequately strong to withstand routine orthodontic and estimated masticatory forces."}
{"id": "PMID:1099145", "title": "An outbreak of dysentery caused by Shigella dysenteriae type 1 on a coral island in the Bay of Bengal.", "content": "An epidemic of severe dysentery occurred on St. Martin Island in the Bay of Bengal at a time when the island, with a population of 1,318, was isolated from the mainland because of the monsoon season. There were 434 cases and 28 deaths within a three-month period. Mortality (2%) was limited to the very young and the elderly. Shigella dysenteriae type 1 (Shiga bacillus) was isolated from 12 of 65 rectal swab specimens. All strains gave similar biochemical reactions and were resistant to tetracycline, chloramphenicol, streptomycin, and the sulfonamides but were sensitive to ampicillin, kanamycin, and gentamicin. No other organism was implicated in the outbreak. The disease disappeared from the island after institution of chlorination of the sources of drinking water and effective treatment of patients.", "contents": "An outbreak of dysentery caused by Shigella dysenteriae type 1 on a coral island in the Bay of Bengal. An epidemic of severe dysentery occurred on St. Martin Island in the Bay of Bengal at a time when the island, with a population of 1,318, was isolated from the mainland because of the monsoon season. There were 434 cases and 28 deaths within a three-month period. Mortality (2%) was limited to the very young and the elderly. Shigella dysenteriae type 1 (Shiga bacillus) was isolated from 12 of 65 rectal swab specimens. All strains gave similar biochemical reactions and were resistant to tetracycline, chloramphenicol, streptomycin, and the sulfonamides but were sensitive to ampicillin, kanamycin, and gentamicin. No other organism was implicated in the outbreak. The disease disappeared from the island after institution of chlorination of the sources of drinking water and effective treatment of patients."}
{"id": "PMID:1099136", "title": "Effect of radiation-induced xerostomia on human oral microflora.", "content": "A longitudinal study was performed to assess the effects of radiation-induced xerostomia on the human oral microflora. Pronounced microbial population shifts were found in each of five intraoral sites tested. Cariogenic microorganisms gained prominence at the expense of noncariogenic microorganisms in concert with the saliva shutdown. These changes occurred before the onset of clinical caries irrespective of whether or not a topical fluoride gel was used as a caries preventive.", "contents": "Effect of radiation-induced xerostomia on human oral microflora. A longitudinal study was performed to assess the effects of radiation-induced xerostomia on the human oral microflora. Pronounced microbial population shifts were found in each of five intraoral sites tested. Cariogenic microorganisms gained prominence at the expense of noncariogenic microorganisms in concert with the saliva shutdown. These changes occurred before the onset of clinical caries irrespective of whether or not a topical fluoride gel was used as a caries preventive."}
{"id": "PMID:1099146", "title": "Asymptomatic epidemic acquisition of group A Streptococcus: antibody response to extracellular and type-specific antigens.", "content": "The antibody response of 40 institutionalized children involved in an epidemic associated with asymptomatic pharyngeal acquisition of a group A, M-11, T-11 typeable Streptococcus was studied. Titers of antibody to streptolysin O and to deoxyribonuclease B determined in sera collected from patients within two weeks of positive throat cultures were significantly higher than those in sera of controls (P smaller than 0.001). However, there was no rise in antibody titers in sera obtained from these patients after an interval of three weeks. Type-specific antibody to the group A Streptococcus (type M-11) was assayed in the sera of 24 patients. No detectable antibody activity was found either in the initial sera or in sera collected eight months after the epidemic. Thus, the asymptomatic nature of this epidemic could not be attributed to the presence of detectable type-specific antibody in this population at the time of the epidemic. These observations suggest that asymptomatic pharyngeal acquisition of group A Streptococcus may occur in epidemic fashion in certain populations and may not be associated with evidence of an antibody response to the streptococcal organism.", "contents": "Asymptomatic epidemic acquisition of group A Streptococcus: antibody response to extracellular and type-specific antigens. The antibody response of 40 institutionalized children involved in an epidemic associated with asymptomatic pharyngeal acquisition of a group A, M-11, T-11 typeable Streptococcus was studied. Titers of antibody to streptolysin O and to deoxyribonuclease B determined in sera collected from patients within two weeks of positive throat cultures were significantly higher than those in sera of controls (P smaller than 0.001). However, there was no rise in antibody titers in sera obtained from these patients after an interval of three weeks. Type-specific antibody to the group A Streptococcus (type M-11) was assayed in the sera of 24 patients. No detectable antibody activity was found either in the initial sera or in sera collected eight months after the epidemic. Thus, the asymptomatic nature of this epidemic could not be attributed to the presence of detectable type-specific antibody in this population at the time of the epidemic. These observations suggest that asymptomatic pharyngeal acquisition of group A Streptococcus may occur in epidemic fashion in certain populations and may not be associated with evidence of an antibody response to the streptococcal organism."}
{"id": "PMID:1099147", "title": "Susceptibility of coccidioides immitis, Candida albicans, and Cryptococcus neoformans to amphotericin B, flucytosine, and clotrimazole.", "content": "Toxicity and failure of treatment with amphotericin B are stimuli for researchers to evaluate alternative antifungal antimicrobics. Also, data from susceptibility tests of Coccidioides immitis are sparse. With use of a defined, synthetic culture medium, C. immitis (25 strains). Candida albicans (21 strains), and Cryptococcus neoformans (21 strains) were tested against flucytosine, clotrimazole, and amphotericin B. Molecule for molecule, the sequency of activity was: clotrimazole greater than amphotericin B greater than flucytosine (totally inactive) C. immitis; and clotrimazole greater than amphotericin B greater than flucytosine with C. albicans and C. neoformans. With four strains of C. immitis, the minimal inhibitory concentration (of amphotericin B) was the same when inocula of arthrospores were tested as when corresponding spherules/endospores were tested simultaneously and identically. The clinical outcome of coccidioidomycosis in 17 patients treated with amphotericin B correlated best with minimal inhibitory concentration after incubation of cultures for 48 hr; a favorable response was associated with minimal inhibitory concentrations of less than or equal 1.0 mug/ml. Because clinical isolates of fungi appear to vary in susceptibility, in vitro tests may have clinical utility.", "contents": "Susceptibility of coccidioides immitis, Candida albicans, and Cryptococcus neoformans to amphotericin B, flucytosine, and clotrimazole. Toxicity and failure of treatment with amphotericin B are stimuli for researchers to evaluate alternative antifungal antimicrobics. Also, data from susceptibility tests of Coccidioides immitis are sparse. With use of a defined, synthetic culture medium, C. immitis (25 strains). Candida albicans (21 strains), and Cryptococcus neoformans (21 strains) were tested against flucytosine, clotrimazole, and amphotericin B. Molecule for molecule, the sequency of activity was: clotrimazole greater than amphotericin B greater than flucytosine (totally inactive) C. immitis; and clotrimazole greater than amphotericin B greater than flucytosine with C. albicans and C. neoformans. With four strains of C. immitis, the minimal inhibitory concentration (of amphotericin B) was the same when inocula of arthrospores were tested as when corresponding spherules/endospores were tested simultaneously and identically. The clinical outcome of coccidioidomycosis in 17 patients treated with amphotericin B correlated best with minimal inhibitory concentration after incubation of cultures for 48 hr; a favorable response was associated with minimal inhibitory concentrations of less than or equal 1.0 mug/ml. Because clinical isolates of fungi appear to vary in susceptibility, in vitro tests may have clinical utility."}
{"id": "PMID:1099148", "title": "Transmissible drug resistance of Escherichia coli and Salmonella from humans, animals, and their rural environments.", "content": "Isolates of Escherichia coli and Salmonella species obtained from humans, animals, and their rural environments were tested for their ability to transfer multiple drug resistance to competent recipients. In all, 125 (52.9%) of 242 isolates of E.coli transferred all or part of their resistance to E.coli CSH-2, and 103 (44.4%) of 232 isolates of E.coli transferred all or part of their resistance to Salmonella choleraesuis var. kuzendorf 214. The data suggest that the multiple drug resistance of E.coli transferred all or part of their resistance to Salmonella choleraesuis var kuzendorf 214. The data suggest that the multiple drug resistance of E. coli in the gut of humans who work with livestock may be due to the transfer of resistance determinants from animal strains to the resident flora of the human gut. Such transfer could occur during transient passage through the digestive tract. Multiple drug-resistant Salmonella were isolated from all sources. In all, 48 (80%) of 60 isolates of Salmonella transferred all or part of their resistance to E. coli CSH-2, and isolates of Salmonella transferred all or part of their resistance to E.coli CSH-2, and 42 (82.4%) of 51 isolates of Salmonella transferred all or part of their resistance to S. choleraesuis var, kuzendorf 214. Fifty-three (46.9%) of 113 antibiotic-sensitive isolates of E.coli possessed resistance transfer factor, and six (23.1%) of 26 isolates with resistance transfer factor mobilized resistance determinants in isolates unable to transfer drug resistance during conjugation experiments.", "contents": "Transmissible drug resistance of Escherichia coli and Salmonella from humans, animals, and their rural environments. Isolates of Escherichia coli and Salmonella species obtained from humans, animals, and their rural environments were tested for their ability to transfer multiple drug resistance to competent recipients. In all, 125 (52.9%) of 242 isolates of E.coli transferred all or part of their resistance to E.coli CSH-2, and 103 (44.4%) of 232 isolates of E.coli transferred all or part of their resistance to Salmonella choleraesuis var. kuzendorf 214. The data suggest that the multiple drug resistance of E.coli transferred all or part of their resistance to Salmonella choleraesuis var kuzendorf 214. The data suggest that the multiple drug resistance of E. coli in the gut of humans who work with livestock may be due to the transfer of resistance determinants from animal strains to the resident flora of the human gut. Such transfer could occur during transient passage through the digestive tract. Multiple drug-resistant Salmonella were isolated from all sources. In all, 48 (80%) of 60 isolates of Salmonella transferred all or part of their resistance to E. coli CSH-2, and isolates of Salmonella transferred all or part of their resistance to E.coli CSH-2, and 42 (82.4%) of 51 isolates of Salmonella transferred all or part of their resistance to S. choleraesuis var, kuzendorf 214. Fifty-three (46.9%) of 113 antibiotic-sensitive isolates of E.coli possessed resistance transfer factor, and six (23.1%) of 26 isolates with resistance transfer factor mobilized resistance determinants in isolates unable to transfer drug resistance during conjugation experiments."}
{"id": "PMID:1099149", "title": "Colicin types of Shigella sonnei in relation to antibiotic resistance.", "content": "Colicin typing of 436 strains of Shigella sonnei isolated in Dallas during a 10-year period was performed to determine whether resistance to ampicillin was associated with a single strain or was widespread among all S. sonnei types. One hundred ninety-three strains collected during a period of eight  and one-half years when ampicillin resistance was 1.6% were available for retrospective review. During the last one and one-half years of study, 20.6% of the 243 strains that represented almost all S. sonnei isolated in Dallas were resistant to ampicillin. More than 50% of the strains collected annually were colicin type 9,22% were untypable, and seven other types were less frequently encountered. Resistance to ampicillin increased slightly from 1.5% to 9.5% among the type 9 strains, and one type 8 strain (2.9%) was resistaiple resistance to seven antibiotics tested was found mainly in untypable strains. Thus it appears that the abrupt increase in ampicillin resistance in mid-1972 was due principally to resistance in a single biotype, and that resistance is not widespread among all types of S. sonnei.", "contents": "Colicin types of Shigella sonnei in relation to antibiotic resistance. Colicin typing of 436 strains of Shigella sonnei isolated in Dallas during a 10-year period was performed to determine whether resistance to ampicillin was associated with a single strain or was widespread among all S. sonnei types. One hundred ninety-three strains collected during a period of eight  and one-half years when ampicillin resistance was 1.6% were available for retrospective review. During the last one and one-half years of study, 20.6% of the 243 strains that represented almost all S. sonnei isolated in Dallas were resistant to ampicillin. More than 50% of the strains collected annually were colicin type 9,22% were untypable, and seven other types were less frequently encountered. Resistance to ampicillin increased slightly from 1.5% to 9.5% among the type 9 strains, and one type 8 strain (2.9%) was resistaiple resistance to seven antibiotics tested was found mainly in untypable strains. Thus it appears that the abrupt increase in ampicillin resistance in mid-1972 was due principally to resistance in a single biotype, and that resistance is not widespread among all types of S. sonnei."}
{"id": "PMID:1099159", "title": "Regression of cutaneous neoplasms following delayed-type hypersensitivity challenge reactions to microbial antigens or lymphokines.", "content": "Induction of delayed-type hypersensitivity challenge reactions to microbial antigens at sites of neoplasms involving the skin resulted in regression of mycosis fungoides, reticulum cell sarcoma, superficial basal cell carcinoma and adenocarcinoma of the breast. Similar reactions induced by lymphokine preparations also resulted in regression of lesions of mycosis fungoides and superficial basal cell carcinoma. The role of the large monomuclear cells in the inflammatory infiltrate of the delayed hypersensitivity reaction in eliciting the tumor regression is discussed. It is proposed that these large mononuclear cells represent the effectors in a primitive surveillance mechanism for neoplastic cells.", "contents": "Regression of cutaneous neoplasms following delayed-type hypersensitivity challenge reactions to microbial antigens or lymphokines. Induction of delayed-type hypersensitivity challenge reactions to microbial antigens at sites of neoplasms involving the skin resulted in regression of mycosis fungoides, reticulum cell sarcoma, superficial basal cell carcinoma and adenocarcinoma of the breast. Similar reactions induced by lymphokine preparations also resulted in regression of lesions of mycosis fungoides and superficial basal cell carcinoma. The role of the large monomuclear cells in the inflammatory infiltrate of the delayed hypersensitivity reaction in eliciting the tumor regression is discussed. It is proposed that these large mononuclear cells represent the effectors in a primitive surveillance mechanism for neoplastic cells."}
{"id": "PMID:1099164", "title": "VECTAN II: A computer program for the spatial analysis of the vectorcardiogram.", "content": "This paper presents the operation of a digital computer program, VECTAN II, for the spatial analysis of the vectorcardiogram (VCG). The program incorporates a unique waveform recognition algorithm based on the spatial vector length which has been shown to perform better than previous algorithms. The waveform analysis employed by the program considers the vectorcardiogram as a three dimensional entity rather than as scalar or planar representations. VECTAN II is designed chiefly to measure and quantify the VCG response of normal subjects to a controlled stress by analyzing one VCG complex every five seconds throughout a long experiment. The program has been used to analyzeom the NASA Johnson Space Center Cardiovascular Laboratory, from the pre- and postflight medical examinations of the Apollo 15, 16 and 17 crewmen, and from onboard Skylab experiments.", "contents": "VECTAN II: A computer program for the spatial analysis of the vectorcardiogram. This paper presents the operation of a digital computer program, VECTAN II, for the spatial analysis of the vectorcardiogram (VCG). The program incorporates a unique waveform recognition algorithm based on the spatial vector length which has been shown to perform better than previous algorithms. The waveform analysis employed by the program considers the vectorcardiogram as a three dimensional entity rather than as scalar or planar representations. VECTAN II is designed chiefly to measure and quantify the VCG response of normal subjects to a controlled stress by analyzing one VCG complex every five seconds throughout a long experiment. The program has been used to analyzeom the NASA Johnson Space Center Cardiovascular Laboratory, from the pre- and postflight medical examinations of the Apollo 15, 16 and 17 crewmen, and from onboard Skylab experiments."}
{"id": "PMID:1099170", "title": "The production of a temperature-sensitive persistent measles virus infection.", "content": "A persistent infection of measles virus was established in HEp2 cells. All cells contained virus antigen when tested by specific immunofluorescence and approx. 50% were positive by haemadsorption. Infectious virus released into the supernatant medium was usually equivalent to no more than 0-001 p.f.u./cell, but between 10 and 40% of the infected cells produced plaques when plated on Vero cells. Passage of persistently infected cultures in the presence of measles antibody had no effect on the proportion of antigen-positive cells. Virus obtained from the supernatant medium of persistently infected cultures was temperature sensitive at 39-5 degrees C when tested on Vero cells whereas the original non-persistent virus produced infections on Vero cells at 39-8 degrees C. On passage of the persistently infected culture at 39-5 degrees C most of the surface antigens disappeared within 24 h whereas the intracellular virus antigens had not totally disappeared until the 5th passage.", "contents": "The production of a temperature-sensitive persistent measles virus infection. A persistent infection of measles virus was established in HEp2 cells. All cells contained virus antigen when tested by specific immunofluorescence and approx. 50% were positive by haemadsorption. Infectious virus released into the supernatant medium was usually equivalent to no more than 0-001 p.f.u./cell, but between 10 and 40% of the infected cells produced plaques when plated on Vero cells. Passage of persistently infected cultures in the presence of measles antibody had no effect on the proportion of antigen-positive cells. Virus obtained from the supernatant medium of persistently infected cultures was temperature sensitive at 39-5 degrees C when tested on Vero cells whereas the original non-persistent virus produced infections on Vero cells at 39-8 degrees C. On passage of the persistently infected culture at 39-5 degrees C most of the surface antigens disappeared within 24 h whereas the intracellular virus antigens had not totally disappeared until the 5th passage."}
{"id": "PMID:1099171", "title": "Beta blockade in lithium tremor.", "content": "Practolol, propranolol, and placebo have been tested on an objective test of lithium induced tremor. Both beta-blocking agents produced significantly more tremor than the placebo. It is argued that lithium induced tremor is closer to essential than to physiological tremor.", "contents": "Beta blockade in lithium tremor. Practolol, propranolol, and placebo have been tested on an objective test of lithium induced tremor. Both beta-blocking agents produced significantly more tremor than the placebo. It is argued that lithium induced tremor is closer to essential than to physiological tremor."}
{"id": "PMID:1099172", "title": "Apomorphine and psychopathology.", "content": "Forty men, mainly alcoholics, were administered either the dopamine receptor agonist, apomorphine HCl (1 mg), or distilled water subcutaneously three times a day for 14 days in a double blind study. None of the subjects developed an endogenous depression or schizophrenic symptoms. Scores on the Hamilton Rating Scale, Zung Self Rating Scale, and Brief Psychiatric Rating Scale showed improvement with both apomorphine and placebo. There were no significant differences between the two treatments on these rating scales. A significant incidence of spontaneous penile erections occurred after apomorphine treatment compared with placebo. Both treatments eliminated subjective craving for alcohol. Acute administration of apomorphine had no effect on psychomotor retardation or depressed mood in two patients with endogenous depression.", "contents": "Apomorphine and psychopathology. Forty men, mainly alcoholics, were administered either the dopamine receptor agonist, apomorphine HCl (1 mg), or distilled water subcutaneously three times a day for 14 days in a double blind study. None of the subjects developed an endogenous depression or schizophrenic symptoms. Scores on the Hamilton Rating Scale, Zung Self Rating Scale, and Brief Psychiatric Rating Scale showed improvement with both apomorphine and placebo. There were no significant differences between the two treatments on these rating scales. A significant incidence of spontaneous penile erections occurred after apomorphine treatment compared with placebo. Both treatments eliminated subjective craving for alcohol. Acute administration of apomorphine had no effect on psychomotor retardation or depressed mood in two patients with endogenous depression."}
{"id": "PMID:1099174", "title": "Threshold for pain from anterolateral quadrant stimulation as a predictor of success of percutaneous cordotomy for relief of pain.", "content": "A sensitive quantitative index for predicting optimal electrode position in percutaneous anterolateral cordotomy was determined by electrical stimulation through the lesioning electrode. If the threshold for pain elicited by the stimulation electrode was less than 300 muA, a 5-sec radiofrequency lesion of 50 mA would produce complete contralateral analgesia with no weakness. When the pain threshold exceeded 300 muA, incomplete or no analgesia would result with the standard single lesion. The results further suggested that the fibers in the anterolateral quadrant that transmit pain are discretely rather than diffusely localized.", "contents": "Threshold for pain from anterolateral quadrant stimulation as a predictor of success of percutaneous cordotomy for relief of pain. A sensitive quantitative index for predicting optimal electrode position in percutaneous anterolateral cordotomy was determined by electrical stimulation through the lesioning electrode. If the threshold for pain elicited by the stimulation electrode was less than 300 muA, a 5-sec radiofrequency lesion of 50 mA would produce complete contralateral analgesia with no weakness. When the pain threshold exceeded 300 muA, incomplete or no analgesia would result with the standard single lesion. The results further suggested that the fibers in the anterolateral quadrant that transmit pain are discretely rather than diffusely localized."}
{"id": "PMID:1099175", "title": "Meningomyelocele and progressive hydromyelia. Progressive paresis in myelodysplasia.", "content": "Five cases of myelodysplasia with progressive paraparesis are presented. Three of the five patients developed spasticity, but dissociated sensory loss and loss of sphincter control was not a prominent feature. All were found to have compensated hydrocephalus and extensive communicating hydromyelia. The use of myelography and ventriculography in the diagnosis of hydromyelia is discussed. Ventricular drainage led to clinical improvement in two cases and radiological improvement in one. The relationship of compensated hydrocephalus, meningomyelocele, and progressive hydromyelia postnatally may support the hydrodynamic hypothesis of myelodysplasia.", "contents": "Meningomyelocele and progressive hydromyelia. Progressive paresis in myelodysplasia. Five cases of myelodysplasia with progressive paraparesis are presented. Three of the five patients developed spasticity, but dissociated sensory loss and loss of sphincter control was not a prominent feature. All were found to have compensated hydrocephalus and extensive communicating hydromyelia. The use of myelography and ventriculography in the diagnosis of hydromyelia is discussed. Ventricular drainage led to clinical improvement in two cases and radiological improvement in one. The relationship of compensated hydrocephalus, meningomyelocele, and progressive hydromyelia postnatally may support the hydrodynamic hypothesis of myelodysplasia."}
{"id": "PMID:1099176", "title": "Effect of ascorbic acid on the human electroencephalogram.", "content": "Controversy exists over whether ascorbic acid (vitamin C) in doses in excess of known physiological requirements has demonstrable biological effects. The present study reports that such megadoses of vitamin C do significantly effect an objective electrophysiological measure, i.e., electroencephalogram (EEG) driving responses to photic stimulation. Four thousand milligrams of vitamin C administered 27 and 3 hours prior to assessment produced an effect on the EEGs of 18 healthy adolescent human males that was significantly different from a placebo. Fifty milligrams of ascorbic acid administered in this manner did not produce an effect on the EEG significantly different from placebo administration.", "contents": "Effect of ascorbic acid on the human electroencephalogram. Controversy exists over whether ascorbic acid (vitamin C) in doses in excess of known physiological requirements has demonstrable biological effects. The present study reports that such megadoses of vitamin C do significantly effect an objective electrophysiological measure, i.e., electroencephalogram (EEG) driving responses to photic stimulation. Four thousand milligrams of vitamin C administered 27 and 3 hours prior to assessment produced an effect on the EEGs of 18 healthy adolescent human males that was significantly different from a placebo. Fifty milligrams of ascorbic acid administered in this manner did not produce an effect on the EEG significantly different from placebo administration."}
{"id": "PMID:1099180", "title": "The histopathology of lepromatous leprosy in the nose.", "content": "On the basis of clinical, histological and bacteriological assessments, 31 patients in Central India were selected and classified as having active but early lepromatous leprosy and 4 patients as having early borderline leprosy. From the nose of each patient an average of 4 biopsies were taken from particular sites of the septum and turbinates either by punch biopsy or dissection with a scalpel. The nasal tissues from all the lepromatous patients contained many acid-fast bacilli; no bacilli or abnormalities were seen in nasal tissues from the borderline patients. The histopathology of these highly bacilliferous tissues is described. Bacilli were universally seen in macrophages, but they were also seen in blood monocytes and polymorphs, fibroblasts, squamous and pseudo-columnar epithelium, keratin, peri- and endo-neurial cells of tiny nerve bundles, erectile tissue, vascular plain muscle, perivascular histiocytes and frequently and abundantly in the cytoplasm of endothelial lining cells of lymphatics and of small blood vessels and free within the lumina of these vessels. Five basic mechanisms of escape of bacilli from the submucosa on to the surface, and thus into the external environment, are described. Secondary infection, in the presence of an expansile lepromatous infiltrate, together with simple trauma to the surface epithelium, are the main factors in the discharge of bacilli. These histopathological observations are consistent with the findings from other recent studies on the nose in leprosy regarding (1) the large numbers of morphologically intact and viable Myco. leprae excreted in the nasal mucus of lepromatous patients; (2) the clinical changes observed in the nose of such patients, and (3) the similar nasal involvement and excretion of Myco. leprae from the nose of mice inoculated with leprosy bacilli of human origin. Of particular interest was the frequency and intensity of bacilli within the endothelial lining cells of small blood and lymph vessels and the presence of bacilli free within the lumina of these vessels or within monocytes and polymorphs. The possible dynamic significance of these observations in the pathogenesis of leprosy is discussed. The significance of all these observations in relation to (1) the spread of leprosy; (2) local factors in the nose which might favour the growth of Myco. leprae, and (3) the nose as a portal of entry, are discussed.", "contents": "The histopathology of lepromatous leprosy in the nose. On the basis of clinical, histological and bacteriological assessments, 31 patients in Central India were selected and classified as having active but early lepromatous leprosy and 4 patients as having early borderline leprosy. From the nose of each patient an average of 4 biopsies were taken from particular sites of the septum and turbinates either by punch biopsy or dissection with a scalpel. The nasal tissues from all the lepromatous patients contained many acid-fast bacilli; no bacilli or abnormalities were seen in nasal tissues from the borderline patients. The histopathology of these highly bacilliferous tissues is described. Bacilli were universally seen in macrophages, but they were also seen in blood monocytes and polymorphs, fibroblasts, squamous and pseudo-columnar epithelium, keratin, peri- and endo-neurial cells of tiny nerve bundles, erectile tissue, vascular plain muscle, perivascular histiocytes and frequently and abundantly in the cytoplasm of endothelial lining cells of lymphatics and of small blood vessels and free within the lumina of these vessels. Five basic mechanisms of escape of bacilli from the submucosa on to the surface, and thus into the external environment, are described. Secondary infection, in the presence of an expansile lepromatous infiltrate, together with simple trauma to the surface epithelium, are the main factors in the discharge of bacilli. These histopathological observations are consistent with the findings from other recent studies on the nose in leprosy regarding (1) the large numbers of morphologically intact and viable Myco. leprae excreted in the nasal mucus of lepromatous patients; (2) the clinical changes observed in the nose of such patients, and (3) the similar nasal involvement and excretion of Myco. leprae from the nose of mice inoculated with leprosy bacilli of human origin. Of particular interest was the frequency and intensity of bacilli within the endothelial lining cells of small blood and lymph vessels and the presence of bacilli free within the lumina of these vessels or within monocytes and polymorphs. The possible dynamic significance of these observations in the pathogenesis of leprosy is discussed. The significance of all these observations in relation to (1) the spread of leprosy; (2) local factors in the nose which might favour the growth of Myco. leprae, and (3) the nose as a portal of entry, are discussed."}
{"id": "PMID:1099181", "title": "Viral hepatitis with intracytoplasmic cholestasis and cholesterol crystals in a drug addict.", "content": "Electron-microscopical exammination of a liver biopsy from a young female drug addict with clinically relapsing serum hepatitis revealed virus-like particles free in the liver cell cytoplasm. In addition, there was intracellular cholestasis, and numerous clefts were found in the cytoplasm of hepatocytes, presumably caused by the presence of cholesterol crystals which were dissolved during the processing of the tissue. Such clefts have earlier been reported in liver cells in patients with cholestasis and hypercholesterolemia, but not in patients with viral hepatitis and a normal serum cholesterol concentration.", "contents": "Viral hepatitis with intracytoplasmic cholestasis and cholesterol crystals in a drug addict. Electron-microscopical exammination of a liver biopsy from a young female drug addict with clinically relapsing serum hepatitis revealed virus-like particles free in the liver cell cytoplasm. In addition, there was intracellular cholestasis, and numerous clefts were found in the cytoplasm of hepatocytes, presumably caused by the presence of cholesterol crystals which were dissolved during the processing of the tissue. Such clefts have earlier been reported in liver cells in patients with cholestasis and hypercholesterolemia, but not in patients with viral hepatitis and a normal serum cholesterol concentration."}
{"id": "PMID:1099183", "title": "Yeast colonization in hospitalized and nonhospitalized children.", "content": "Data have been accumulated to determine the prevalence of yeast colonization of the skin and digestive tract of hospitalized and nonhospitalized infants and children. There was no difference in the prevalence between hospitalized patients at the time of admission and nonhospitalized children. However, there was a positive correlation of the duration of hospitalization and the prevalence of yeast colonization. There were no correlations of hospitalization with site of colonization, age of the patient, or type of yeast isolated.", "contents": "Yeast colonization in hospitalized and nonhospitalized children. Data have been accumulated to determine the prevalence of yeast colonization of the skin and digestive tract of hospitalized and nonhospitalized infants and children. There was no difference in the prevalence between hospitalized patients at the time of admission and nonhospitalized children. However, there was a positive correlation of the duration of hospitalization and the prevalence of yeast colonization. There were no correlations of hospitalization with site of colonization, age of the patient, or type of yeast isolated."}
{"id": "PMID:1099184", "title": "Early versus late introduction of continuous negative pressure in the management of the idiopathic respiratory distress syndrome.", "content": "To evaluate the effectiveness of the early application of continuous negative pressure about the thorax, 23 infants with the idiopathic respiratory distress syndrome who had adequate alveolar ventilation were assigned to early or late application of CNP. There were 11 infants in the early CNP and 12 in the late CNP group. There were no differences between the two groups with respect to birth weight, gestational age, age at admission to the study, initial FIO2 requirements, or initial PaO2, PaCo2, and AaPO2. None of the ENCP patients required mechanical ventilation, whereas four of the LCNP group did so. The FIO2 requirements were significantly less for the ECNP infants when compared to the LCNP ones. The mean duration of FIO2 requirements greater than 0.6 was 28.3 hours for those receiving ECNP and 60.7 hours for those in LCNP (P LESS THAN 0.05). This study suggests that the course of RDS may be modified by the early application of CNP.", "contents": "Early versus late introduction of continuous negative pressure in the management of the idiopathic respiratory distress syndrome. To evaluate the effectiveness of the early application of continuous negative pressure about the thorax, 23 infants with the idiopathic respiratory distress syndrome who had adequate alveolar ventilation were assigned to early or late application of CNP. There were 11 infants in the early CNP and 12 in the late CNP group. There were no differences between the two groups with respect to birth weight, gestational age, age at admission to the study, initial FIO2 requirements, or initial PaO2, PaCo2, and AaPO2. None of the ENCP patients required mechanical ventilation, whereas four of the LCNP group did so. The FIO2 requirements were significantly less for the ECNP infants when compared to the LCNP ones. The mean duration of FIO2 requirements greater than 0.6 was 28.3 hours for those receiving ECNP and 60.7 hours for those in LCNP (P LESS THAN 0.05). This study suggests that the course of RDS may be modified by the early application of CNP."}
{"id": "PMID:1099186", "title": "Traumatizing effect of breech delivery on infants with spina bifida.", "content": "About one quarter of babies with myelomeningocele are born by breech delivery. Dissection of the locomotor system tissues of 49 spina bifida babies born by normal delivery and 15 spina bifida babies born by breech delivery revealed that the latter type of labor causes frequent and often severe trauma to the already handicapped muscles and nerves of the lower extremities. In cases where the birth damage is extensive, the baby's chances of survival may be reduced. The possibility should be considered that this type of labor, which traumatizes the baby's muscles and nerves, may influence the postnatal locomotor function and further contribute to the child's disability.", "contents": "Traumatizing effect of breech delivery on infants with spina bifida. About one quarter of babies with myelomeningocele are born by breech delivery. Dissection of the locomotor system tissues of 49 spina bifida babies born by normal delivery and 15 spina bifida babies born by breech delivery revealed that the latter type of labor causes frequent and often severe trauma to the already handicapped muscles and nerves of the lower extremities. In cases where the birth damage is extensive, the baby's chances of survival may be reduced. The possibility should be considered that this type of labor, which traumatizes the baby's muscles and nerves, may influence the postnatal locomotor function and further contribute to the child's disability."}
{"id": "PMID:1099187", "title": "Glucocorticoid prophylaxis for respiratory distress syndrome: a review of potential toxicity.", "content": "Much evidence suggests that glucocorticoids may increase the rate of lung development during late fetal life, and thereby decrease the risk of respiratory distress syndrome if the fetus is born prematurely. Results of human and animal studies are basically in agreement. The risks of this form of therapy are less well examined, especially in human studies. This review briefly outlines studies that relate to toxicity so that the \"risk/benefit ratio\" of this form of therapy may be assessed.", "contents": "Glucocorticoid prophylaxis for respiratory distress syndrome: a review of potential toxicity. Much evidence suggests that glucocorticoids may increase the rate of lung development during late fetal life, and thereby decrease the risk of respiratory distress syndrome if the fetus is born prematurely. Results of human and animal studies are basically in agreement. The risks of this form of therapy are less well examined, especially in human studies. This review briefly outlines studies that relate to toxicity so that the \"risk/benefit ratio\" of this form of therapy may be assessed."}
{"id": "PMID:1099188", "title": "Osseous autografts. I. Clinical responses to bone blend or hip marrow grafts.", "content": "A clinical evaluation was undertaken to compare regeneration of osseous defects following implantation of either osseous coagulum-bone blend from intraoral sources or autogenous iliac marrow-cancellous bone. Thirty-two transplants were performed in 15 male patients. The intraosseous defects in which marrow was placed (initial average depth = 7.18 mm) filled 60.7% (average fill = 4.36 mm). Defects in which osseous coagulum-bone blend was placed (initial average depth 4.0 mm) filled 73% (average fill = 2.93 mm). The difference in results between the two materials was not statistically significant. Therefore, similar levels of osseous regeneration apparently took place regardless of graft material used.", "contents": "Osseous autografts. I. Clinical responses to bone blend or hip marrow grafts. A clinical evaluation was undertaken to compare regeneration of osseous defects following implantation of either osseous coagulum-bone blend from intraoral sources or autogenous iliac marrow-cancellous bone. Thirty-two transplants were performed in 15 male patients. The intraosseous defects in which marrow was placed (initial average depth = 7.18 mm) filled 60.7% (average fill = 4.36 mm). Defects in which osseous coagulum-bone blend was placed (initial average depth 4.0 mm) filled 73% (average fill = 2.93 mm). The difference in results between the two materials was not statistically significant. Therefore, similar levels of osseous regeneration apparently took place regardless of graft material used."}
{"id": "PMID:1099189", "title": "The clinical and histological response of periodontal pockets to root planing and oral hygiene.", "content": "The study evaluated, clinically and microscopically, the soft tissue response of suprabony periodontal pockets treated by root planing and oral hygiene or by oral hygiene measures alone. The participants were 22 adults beginning treatment for inflammatory periodontal disease. For each patient, three clinically similar labial or lingual pockets were scored for gingival inflammation, pocket depth, and the extent of plaque and calculus. One pocket in each patient served as a control, and a gingival biopsy was obtained to determine the pretreatment level of inflammation. The second pocket was treated by root planing and by the patient's oral hygiene measures. The third pocket was treated only by the patient's daily oral hygiene measures. Fifty-six to 63 days later, treated areas were reevaluated clinically, and biopsies were secured. Each type of therapy reduced the mean pocket depth and the incidence and severity of gingivitis; however, root planing accompanied by oral hygiene measures resulted in a statistically greater improvement than did oral hygiene measures alone.", "contents": "The clinical and histological response of periodontal pockets to root planing and oral hygiene. The study evaluated, clinically and microscopically, the soft tissue response of suprabony periodontal pockets treated by root planing and oral hygiene or by oral hygiene measures alone. The participants were 22 adults beginning treatment for inflammatory periodontal disease. For each patient, three clinically similar labial or lingual pockets were scored for gingival inflammation, pocket depth, and the extent of plaque and calculus. One pocket in each patient served as a control, and a gingival biopsy was obtained to determine the pretreatment level of inflammation. The second pocket was treated by root planing and by the patient's oral hygiene measures. The third pocket was treated only by the patient's daily oral hygiene measures. Fifty-six to 63 days later, treated areas were reevaluated clinically, and biopsies were secured. Each type of therapy reduced the mean pocket depth and the incidence and severity of gingivitis; however, root planing accompanied by oral hygiene measures resulted in a statistically greater improvement than did oral hygiene measures alone."}
{"id": "PMID:1099192", "title": "Cryopreservation of Trypanosoma cruzi bloodstream forms.", "content": "Recovery rates of T. cruzi bloodstream forms subjected to several methods of cryopreservation in liquid nitrogen and at -73 C are reported.  Inoculations of animals with cryopreserved and nonpreserved trypomastigotes revealed that prolonged storage at -196 C apparently did not change the biologic characteristics of different T. cruzi strains.  The reproducibility and consistency of results suggest that \"cryobanks\" or \"reference centers\" may be established.", "contents": "Cryopreservation of Trypanosoma cruzi bloodstream forms. Recovery rates of T. cruzi bloodstream forms subjected to several methods of cryopreservation in liquid nitrogen and at -73 C are reported.  Inoculations of animals with cryopreserved and nonpreserved trypomastigotes revealed that prolonged storage at -196 C apparently did not change the biologic characteristics of different T. cruzi strains.  The reproducibility and consistency of results suggest that \"cryobanks\" or \"reference centers\" may be established."}
{"id": "PMID:1099195", "title": "Ovulation induction and antifertility effects of an LH-RH analogue (AY 25,205) in cyclic rats.", "content": "Pro-oestrous rats, treated with fluphenazine dihydrochloride to block ovulation, were used to compare the ovulation-inducing activity of synthetic LH-RH with one of its analogues, [D-Ala-6, des-Gly-NH2-10]-LH-RH ethylamide (AY-25,205). The lowest dose of LH-RH which produced a significant response was 125 ng compared with 2.9 ng AY-25,205. Statistical analysis of the data showed that AY-25,205 was approximately thirty-six times more potent than LH-RH. Treatment with AY-25,205 also induced partial ovulation in metoestrous rats (80 or 160 ng/rat) and apparently normal ovulation in dioestrous rats (10 to 160 ng/rat). The compound (160 ng) failed to induce further ovulation in oestrous rats which ovulated during the previous night. Advancement of premature ovulation in dioestrous rats with AY-25,205 prevented mating behaviour and pregnancy during the treatment cycle. The antifertility effect of the compound disappeared during the following cycle.", "contents": "Ovulation induction and antifertility effects of an LH-RH analogue (AY 25,205) in cyclic rats. Pro-oestrous rats, treated with fluphenazine dihydrochloride to block ovulation, were used to compare the ovulation-inducing activity of synthetic LH-RH with one of its analogues, [D-Ala-6, des-Gly-NH2-10]-LH-RH ethylamide (AY-25,205). The lowest dose of LH-RH which produced a significant response was 125 ng compared with 2.9 ng AY-25,205. Statistical analysis of the data showed that AY-25,205 was approximately thirty-six times more potent than LH-RH. Treatment with AY-25,205 also induced partial ovulation in metoestrous rats (80 or 160 ng/rat) and apparently normal ovulation in dioestrous rats (10 to 160 ng/rat). The compound (160 ng) failed to induce further ovulation in oestrous rats which ovulated during the previous night. Advancement of premature ovulation in dioestrous rats with AY-25,205 prevented mating behaviour and pregnancy during the treatment cycle. The antifertility effect of the compound disappeared during the following cycle."}
{"id": "PMID:1099198", "title": "Cellular and subcellular compartmentalization of steroid metabolism in the rat testis.", "content": "The following aspects are discussed in relation to the cellular and subcellular localization of steroid metabolism in the rat testis: (1) the localization of enzyme activities for steroid production in interstitial tissue and seminiferous tubules in intact cells and in isolated mitochondrial fractions: (2) steroid production by isolated cellular and subcellular fractions and by the intact testis in vivo; and (3) the effect of cofactors on steroid production by rat testis preparations in vitro. It is concluded that it is difficult to decide whether steroid production and the enzymes involved in steroid metabolism are restricted to specific compartments. Steroid production by the testis can, however, be quantitatively explained by that occurring in the isolated mitochondria of the interstitial tissue.", "contents": "Cellular and subcellular compartmentalization of steroid metabolism in the rat testis. The following aspects are discussed in relation to the cellular and subcellular localization of steroid metabolism in the rat testis: (1) the localization of enzyme activities for steroid production in interstitial tissue and seminiferous tubules in intact cells and in isolated mitochondrial fractions: (2) steroid production by isolated cellular and subcellular fractions and by the intact testis in vivo; and (3) the effect of cofactors on steroid production by rat testis preparations in vitro. It is concluded that it is difficult to decide whether steroid production and the enzymes involved in steroid metabolism are restricted to specific compartments. Steroid production by the testis can, however, be quantitatively explained by that occurring in the isolated mitochondria of the interstitial tissue."}
{"id": "PMID:1099200", "title": "Antimalarial phenanthrene amino alcohols. 3. Halogen-containing 9-phenanthrenemethanols.", "content": "A series of new 9-phenanthrene amino alcohols has been prepared in which each compound bears from one to five halogen or halogen-containing moieties. A number of these compounds are extremely active against Plasmodium berghei in the mouse. Some structural requirements for optimal efficacy are considered.", "contents": "Antimalarial phenanthrene amino alcohols. 3. Halogen-containing 9-phenanthrenemethanols. A series of new 9-phenanthrene amino alcohols has been prepared in which each compound bears from one to five halogen or halogen-containing moieties. A number of these compounds are extremely active against Plasmodium berghei in the mouse. Some structural requirements for optimal efficacy are considered."}
{"id": "PMID:1099201", "title": "Synthesis and antibacterial evaluation of 1,2,3,4-tetrahydro-4-oxo-1,8-naphthyridine-3-carboxylic acid esters, carbonitriles, and carboxamides.", "content": "A series of 1,2,3,4-tetrahydro-4-oxo-1,8-naphthyridine-3-carboxylic acid esters, carbonitriles, and carboxamides (2a-k) was synthesized and initially evaluated (dose range 50-400 mg/kg) in mice infected with Escherichia coli. Only two derivatives, the ethyl and butyl esters of 1-ethyl-1,2-dihydro-4-hydroxy-7-methyl-1,8-naphthyridine-3-carboxylic acid, protected the animals against E. coli and several other gram-negative bacterial pathogenic infections. A pro-drug type of mechanism appears to be operable since neither agent showed in vitro activity.", "contents": "Synthesis and antibacterial evaluation of 1,2,3,4-tetrahydro-4-oxo-1,8-naphthyridine-3-carboxylic acid esters, carbonitriles, and carboxamides. A series of 1,2,3,4-tetrahydro-4-oxo-1,8-naphthyridine-3-carboxylic acid esters, carbonitriles, and carboxamides (2a-k) was synthesized and initially evaluated (dose range 50-400 mg/kg) in mice infected with Escherichia coli. Only two derivatives, the ethyl and butyl esters of 1-ethyl-1,2-dihydro-4-hydroxy-7-methyl-1,8-naphthyridine-3-carboxylic acid, protected the animals against E. coli and several other gram-negative bacterial pathogenic infections. A pro-drug type of mechanism appears to be operable since neither agent showed in vitro activity."}
{"id": "PMID:1099202", "title": "Inhibitors of protein synthesis. 4. Studies on the structure-activity relationship of gougerotin and some of its analogs.", "content": "Seventeen structural analogs of gougerotin have been compared with the parent compound as inhibitors of the growh of E. coli B and as inhibitors of N-acetylphenylalanylpuromycin formation. The analogs comprise compounds with (1) an intact sarcosyl-D-seryl moiety and modifications at various positions of the carbohydrate moiety, (2) compounds which lack the peptidyl residue, and (3) compounds in which the sarcosyl-D-seryl moiety has been replaced by other aminoacyl or peptidyl moieties and in which various sites in the carbohydrate moiety have been modified in addition. These comparisons revealed that (1) the 3'-hydroxyl group makes a strong contribution to inhibitory activity, (2) the introduction of a double bond between the 2' and 3' positions of the carbohydrate moiety causes a marked decrease in activity; however, if in addition the sarcosyl-D-seryl moiety is replaced by the epsilon-N-methyl-beta-L-arginyl side chain, full activity is restored, (3) there is a marked degree of stereospecificity around the alpha carbon of the seryl moiety (replacement of the sarcosyl-D-seryl residue with the sarcosyl-L-seryl residue results in greatly reduced activity), and (4) replacement of the carboxamide group at position 5' with a hydroxymethyl function resulsts in reduced activity which is further decreased by alterations in the sarcosyl-D-seryl moiety. These alterations include (a) replacement of the D-seryl residue of the sarcosyl-D-seryl moiety by the D-alanyl or the D-phenylalanyl residues and (b) replacement of the whole sarcosyl-D-seryl side chain by the D-seryl, D-alanyl, D-phenylalanyl, p-methoxy-D-phenylalanyl, or p-methoxy-L-phenylalanyl moieties; the replacement of the sarcosyl-D-seryl side chain with the various aminoacyl residues did not lead to acceptor (puromycin-like) activity with N-Ac-Phe-tRNA as the donor.", "contents": "Inhibitors of protein synthesis. 4. Studies on the structure-activity relationship of gougerotin and some of its analogs. Seventeen structural analogs of gougerotin have been compared with the parent compound as inhibitors of the growh of E. coli B and as inhibitors of N-acetylphenylalanylpuromycin formation. The analogs comprise compounds with (1) an intact sarcosyl-D-seryl moiety and modifications at various positions of the carbohydrate moiety, (2) compounds which lack the peptidyl residue, and (3) compounds in which the sarcosyl-D-seryl moiety has been replaced by other aminoacyl or peptidyl moieties and in which various sites in the carbohydrate moiety have been modified in addition. These comparisons revealed that (1) the 3'-hydroxyl group makes a strong contribution to inhibitory activity, (2) the introduction of a double bond between the 2' and 3' positions of the carbohydrate moiety causes a marked decrease in activity; however, if in addition the sarcosyl-D-seryl moiety is replaced by the epsilon-N-methyl-beta-L-arginyl side chain, full activity is restored, (3) there is a marked degree of stereospecificity around the alpha carbon of the seryl moiety (replacement of the sarcosyl-D-seryl residue with the sarcosyl-L-seryl residue results in greatly reduced activity), and (4) replacement of the carboxamide group at position 5' with a hydroxymethyl function resulsts in reduced activity which is further decreased by alterations in the sarcosyl-D-seryl moiety. These alterations include (a) replacement of the D-seryl residue of the sarcosyl-D-seryl moiety by the D-alanyl or the D-phenylalanyl residues and (b) replacement of the whole sarcosyl-D-seryl side chain by the D-seryl, D-alanyl, D-phenylalanyl, p-methoxy-D-phenylalanyl, or p-methoxy-L-phenylalanyl moieties; the replacement of the sarcosyl-D-seryl side chain with the various aminoacyl residues did not lead to acceptor (puromycin-like) activity with N-Ac-Phe-tRNA as the donor."}
{"id": "PMID:1099203", "title": "1(((5-Nitrofuranyl)methylene)amino)-4 and/or-5-substituted 2-imidazolidinones.", "content": "A series of 1-[[(5-nitrofuranyl)methylene]amino]-4- and/or -5-substituted 2-imidazolidinones was prepared utilizing three different reaction sequences. The structure of 4, the product derived from 4-methyl-2-imidazolidinone (2a), was verified by synthesis using an alternate, unequivocal route. The levo isomer l-4 was prepared by a series of reactions starting with L(+)-2-amino-1-propanol (l-10). All of the nitrofurans were examined for potential use as chemotherapeutic agents for urinary tract infections. Based on the high level of activity in the urine and the in vitro antibacterial activity (MIC) 4, l-4, and 16 are considered to be the most active as urinary tract agents.", "contents": "1(((5-Nitrofuranyl)methylene)amino)-4 and/or-5-substituted 2-imidazolidinones. A series of 1-[[(5-nitrofuranyl)methylene]amino]-4- and/or -5-substituted 2-imidazolidinones was prepared utilizing three different reaction sequences. The structure of 4, the product derived from 4-methyl-2-imidazolidinone (2a), was verified by synthesis using an alternate, unequivocal route. The levo isomer l-4 was prepared by a series of reactions starting with L(+)-2-amino-1-propanol (l-10). All of the nitrofurans were examined for potential use as chemotherapeutic agents for urinary tract infections. Based on the high level of activity in the urine and the in vitro antibacterial activity (MIC) 4, l-4, and 16 are considered to be the most active as urinary tract agents."}
{"id": "PMID:1099204", "title": "Preparation and biological activity of some aminoacyl and peptidyl derivatives of 2'-amino-2'-deoxyuridine.", "content": "Several aminoacyl and peptidyl derivatives of 2'-amino-2'-deoxyuridine have been synthesized. 2'-Amino-2'-deoxyuridine (I) and 2'-N-glycyl-2'-amino-2'-deoxyuridine (IIIa) were condensed with N-Cbz blocked p-nitrophenyl esters of the appropriate amino acids to afford the protected aminoacyl and peptidyl nucleosides. Removal of the protecting groups gave the title compounds in good yields. The biological effects of these derivatives are discussed.", "contents": "Preparation and biological activity of some aminoacyl and peptidyl derivatives of 2'-amino-2'-deoxyuridine. Several aminoacyl and peptidyl derivatives of 2'-amino-2'-deoxyuridine have been synthesized. 2'-Amino-2'-deoxyuridine (I) and 2'-N-glycyl-2'-amino-2'-deoxyuridine (IIIa) were condensed with N-Cbz blocked p-nitrophenyl esters of the appropriate amino acids to afford the protected aminoacyl and peptidyl nucleosides. Removal of the protecting groups gave the title compounds in good yields. The biological effects of these derivatives are discussed."}
{"id": "PMID:1099207", "title": "A simulation laboratory for medical education.", "content": "Simulation as an instructional technique is being utilized for the learning of clinical skills in the training of health professionals. The Center for Educational Development at the University of Illinois College of Medicine has established a Learning Resource and Development Center to assist medical faculty in the development and evaluation of instructional simulations. These are not designed to replace clinical experiences but to provide additional opportunities for students to strengthen skills in patient examination. Prior to the introduction of these learning tools in 1972, a study was undertaken to validate their instructional effectiveness. After an additional two years of operation and experimentation, a revalidation study was undertaken during the fall of 1974. Improvements are continually being made in the various simulation formats as a result of feedback from students and faculty.", "contents": "A simulation laboratory for medical education. Simulation as an instructional technique is being utilized for the learning of clinical skills in the training of health professionals. The Center for Educational Development at the University of Illinois College of Medicine has established a Learning Resource and Development Center to assist medical faculty in the development and evaluation of instructional simulations. These are not designed to replace clinical experiences but to provide additional opportunities for students to strengthen skills in patient examination. Prior to the introduction of these learning tools in 1972, a study was undertaken to validate their instructional effectiveness. After an additional two years of operation and experimentation, a revalidation study was undertaken during the fall of 1974. Improvements are continually being made in the various simulation formats as a result of feedback from students and faculty."}
{"id": "PMID:1099208", "title": "Interaction of ethidium bromide with the transport system for monovalent cations in yeast.", "content": "Ethidium was found to be taken up by yeast cells in a process that, at certain concentrations has the main following characteristics: a) a substrate is required; b) it presents cooperative kinetics, with n, according to the Hill equation approximately 3; c) ethidium can be concentrated more than 100-fold; d) the uptake is inhibited by Ca2+; e) the uptake of the dye is inhibited by monovalent cations with a selectivity pattern similar to that observed in their transport by yeast; f) ethidium inhibits the uptake of K+, and, at concentrations up to about 250 muM produces a competitive inhibition on the uptake of Rb+; and g) ethidium produces the same effects as K+ on respiration and the extrusion of H+. It is concluded that ethidium is taken up by yeast cells in a selective way by the same transport system normally employed for monovalent cation uptake.", "contents": "Interaction of ethidium bromide with the transport system for monovalent cations in yeast. Ethidium was found to be taken up by yeast cells in a process that, at certain concentrations has the main following characteristics: a) a substrate is required; b) it presents cooperative kinetics, with n, according to the Hill equation approximately 3; c) ethidium can be concentrated more than 100-fold; d) the uptake is inhibited by Ca2+; e) the uptake of the dye is inhibited by monovalent cations with a selectivity pattern similar to that observed in their transport by yeast; f) ethidium inhibits the uptake of K+, and, at concentrations up to about 250 muM produces a competitive inhibition on the uptake of Rb+; and g) ethidium produces the same effects as K+ on respiration and the extrusion of H+. It is concluded that ethidium is taken up by yeast cells in a selective way by the same transport system normally employed for monovalent cation uptake."}
{"id": "PMID:1099218", "title": "The origin of mitochondria.", "content": "The endosymbiont and episome theories about the origin of mitochondria are reviewed.  Biochemical and genetic data, relevant to these theories are discussed.  An alternative theory is also proposed; this theory is that nuclear and mitochondrial DNAs developed from compartmentalized duplicate prokaryote DNAs.", "contents": "The origin of mitochondria. The endosymbiont and episome theories about the origin of mitochondria are reviewed.  Biochemical and genetic data, relevant to these theories are discussed.  An alternative theory is also proposed; this theory is that nuclear and mitochondrial DNAs developed from compartmentalized duplicate prokaryote DNAs."}
{"id": "PMID:1099220", "title": "Foreign-body tumorigenesis: in vitro isolation and expansion of preneoplastic clonal cell populations.", "content": "Foreign-body reactions were induced in coisogenic CBA/H and CBA/H-T6 mice by sc implantation of 15 times 22 times 0.2-mm unplasticized vinyl chloride vinyl acetate copolymer films. At 6 months' post implantation, implants and unopened tissue capsules were transferred to recipient animals of the T6-different partner strain. After another 3 months, part of the film/capsule complex was transferred to (C57BL/10ScSn times CBA/H-T6)F1 mice for tumor development. Capsule-derived and film-attached cells of the other part were separately cultured. Cultures consisting initially of euploid cells were often gradually replaced by different cells with specific aneuploid karyotypes which were identical with, or closely related to, those of the corresponding tumors. The cultured cells implanted in hybrid recipients at different passage numbers frequently gave rise to homologous tumors. Hence, it was possible to prepare in vitro cells with prefixed specific tumor determinants at different stages of preneoplastic maturation.", "contents": "Foreign-body tumorigenesis: in vitro isolation and expansion of preneoplastic clonal cell populations. Foreign-body reactions were induced in coisogenic CBA/H and CBA/H-T6 mice by sc implantation of 15 times 22 times 0.2-mm unplasticized vinyl chloride vinyl acetate copolymer films. At 6 months' post implantation, implants and unopened tissue capsules were transferred to recipient animals of the T6-different partner strain. After another 3 months, part of the film/capsule complex was transferred to (C57BL/10ScSn times CBA/H-T6)F1 mice for tumor development. Capsule-derived and film-attached cells of the other part were separately cultured. Cultures consisting initially of euploid cells were often gradually replaced by different cells with specific aneuploid karyotypes which were identical with, or closely related to, those of the corresponding tumors. The cultured cells implanted in hybrid recipients at different passage numbers frequently gave rise to homologous tumors. Hence, it was possible to prepare in vitro cells with prefixed specific tumor determinants at different stages of preneoplastic maturation."}
{"id": "PMID:1099224", "title": "Clinical applications of free flap transfer in the burn patient.", "content": "Free microvascular flap transfer is indicated in situations in which flap coverage is required and no local flap is available. The technique of transfer of a groin flap based on the superficial circumflex iliac vessels was described. The procedure has been applied to two patients with acute electrical burns with extensive soft-tissue loss, three patients with scar contractures, and one with osteoradionecrosis. Satisfactory results were achieved in all patients. The procedure is contraindicated if recipient or donor vessels are abnormal or inadequate, and in the face of fulminating infection, general debility, and obesity. With scrupulous attention to indications and contraindications, the procedure is safe, reliable, and a valuable addition to the surgeon's armamentarium.", "contents": "Clinical applications of free flap transfer in the burn patient. Free microvascular flap transfer is indicated in situations in which flap coverage is required and no local flap is available. The technique of transfer of a groin flap based on the superficial circumflex iliac vessels was described. The procedure has been applied to two patients with acute electrical burns with extensive soft-tissue loss, three patients with scar contractures, and one with osteoradionecrosis. Satisfactory results were achieved in all patients. The procedure is contraindicated if recipient or donor vessels are abnormal or inadequate, and in the face of fulminating infection, general debility, and obesity. With scrupulous attention to indications and contraindications, the procedure is safe, reliable, and a valuable addition to the surgeon's armamentarium."}
{"id": "PMID:1099225", "title": "Clinical evaluation of the carbon dioxide laser for burn wound excisions: a comparison of the laser, scalpel, and electrocautery.", "content": "The use of a 100 watt carbon dioxide laser for surgical excisions of burn eschar was evaluated in 13 patients with symmetrical burns. In each patient, laser excision was compared with either scalpel or electrocautery excision of a comparable area. Graft \"take,\" blood loss, and operative speed were measured. Graft \"take\" following laser excisions was comparable to that obtained when either scalpel or electrocautery was used. Regression analysis indicates that laser excisions involved 29% of the blood loss encountered with scalpel excisions and that laser speed was 1.49 times scalpel speed. Although laser blood loss was 60% of electrocautery blood loss, laser speed was significantly slower: 73% of electrocautery speed. These differences, however, were of small clinical consequence. It is felt that the surgical arm of this laser is at present quite awkward and is in need of technological refinement if the full potential of the laser is to be realized.", "contents": "Clinical evaluation of the carbon dioxide laser for burn wound excisions: a comparison of the laser, scalpel, and electrocautery. The use of a 100 watt carbon dioxide laser for surgical excisions of burn eschar was evaluated in 13 patients with symmetrical burns. In each patient, laser excision was compared with either scalpel or electrocautery excision of a comparable area. Graft \"take,\" blood loss, and operative speed were measured. Graft \"take\" following laser excisions was comparable to that obtained when either scalpel or electrocautery was used. Regression analysis indicates that laser excisions involved 29% of the blood loss encountered with scalpel excisions and that laser speed was 1.49 times scalpel speed. Although laser blood loss was 60% of electrocautery blood loss, laser speed was significantly slower: 73% of electrocautery speed. These differences, however, were of small clinical consequence. It is felt that the surgical arm of this laser is at present quite awkward and is in need of technological refinement if the full potential of the laser is to be realized."}
{"id": "PMID:1099229", "title": "Regulation of early mRNA synthesis after bacteriophage T4 infection of Escherichia coli.", "content": "Regulation of T4-specific mRNA synthesis was studied during leucine starvation of a leucine-requiring stringent Escherichia coli B strain. This was done by imposing starvation prior to T4 infection and then letting RNA synthesis proceed for different time periods. Rifampin or streptolydigin was added to stop further RNA synthesis, and protein synthesis was restored by addition of leucine. Samples were withdrawn at different times, and the enzyme-forming capacities found that, during conditions which elicit the stringent response in uninfected bacteria, immediate early mRNA is not stringently regulated. This conclusion contradicts the earlier conclusion of others, obtained by measuring incorporation of radioactive uracil; this is explained by  the observation of Edlin and Neuhard (1967), confirmed and extended by us to the T4-infected cell, that the incorporation of uracil into RNA of a stringent strain is virtually blocked by amino acid starvation, whereas that of adenine continues at 30 to 50% of the rate seen in the presence of the required amino acid.", "contents": "Regulation of early mRNA synthesis after bacteriophage T4 infection of Escherichia coli. Regulation of T4-specific mRNA synthesis was studied during leucine starvation of a leucine-requiring stringent Escherichia coli B strain. This was done by imposing starvation prior to T4 infection and then letting RNA synthesis proceed for different time periods. Rifampin or streptolydigin was added to stop further RNA synthesis, and protein synthesis was restored by addition of leucine. Samples were withdrawn at different times, and the enzyme-forming capacities found that, during conditions which elicit the stringent response in uninfected bacteria, immediate early mRNA is not stringently regulated. This conclusion contradicts the earlier conclusion of others, obtained by measuring incorporation of radioactive uracil; this is explained by  the observation of Edlin and Neuhard (1967), confirmed and extended by us to the T4-infected cell, that the incorporation of uracil into RNA of a stringent strain is virtually blocked by amino acid starvation, whereas that of adenine continues at 30 to 50% of the rate seen in the presence of the required amino acid."}
{"id": "PMID:1099230", "title": "In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities.", "content": "Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and from these monomeric, dimeric, or trimeric circular molecules were purified. By transformation of cells with these DNAs, clones were obtained that carried lambdadv1 in a monomeric or dimeric form. The former type of clones have not been generated in vivo, except for one in a different host strain, and carriers of timeric or tetrameric lambdadv1's have not been obtained so far. It was observed that a considerable fraction of these oligomeric circular DNAs were converted to lower oligomers (e.g., from trimer to dimer) during transformation. The characteristics of the monomeric lambdadv1 carriers obtained were compared with those of dimeric lambdadv1 carriers. The stabilities of the plasmids of the two forms were the same. However, the monomeric plasmid carriers were less tolerant to lambdavir phage infection and perpetuated about 30% less plasmid genomes in monomer units. Furthermore, dimeric plasmid carriers appeared spontaneously and accumulated in cultures of the monomeric lambdadv1 carriers.", "contents": "In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities. Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and from these monomeric, dimeric, or trimeric circular molecules were purified. By transformation of cells with these DNAs, clones were obtained that carried lambdadv1 in a monomeric or dimeric form. The former type of clones have not been generated in vivo, except for one in a different host strain, and carriers of timeric or tetrameric lambdadv1's have not been obtained so far. It was observed that a considerable fraction of these oligomeric circular DNAs were converted to lower oligomers (e.g., from trimer to dimer) during transformation. The characteristics of the monomeric lambdadv1 carriers obtained were compared with those of dimeric lambdadv1 carriers. The stabilities of the plasmids of the two forms were the same. However, the monomeric plasmid carriers were less tolerant to lambdavir phage infection and perpetuated about 30% less plasmid genomes in monomer units. Furthermore, dimeric plasmid carriers appeared spontaneously and accumulated in cultures of the monomeric lambdadv1 carriers."}
{"id": "PMID:1099231", "title": "Genetic studies of coliphage P1. I. Mapping by use of prophage deletions.", "content": "One hundred and ten amber mutants of coliphage P1 were isolated and localized into groups with respect to the existing genetic map by use of nonpermissive Escherichia coli K-12 strains lysogenic for P1 with deletions. These lysogens contain one of three types of deletion prophages: P1cry and its derivatives, P1dlacs, and P1dpros. Fourteen such lysogens were tested for their ability to rescue the amber mutants which were then assigned to one of nine deletion segments of the P1 genome defined by the termini of the various prophage deletions. The relationship of the nine deletion segments with the published P1 map is described, two new segments having been added. The deletions of the 14 prophages overlapped sufficiently to indicate that the P1 genetic prophage map should be represented in circular form, which is consistent with the fact that P1 is normally a circular plasmid in the prophage state. The distribution of mutants into deletion segments is nonrandom for at least one segment. In addition, the deletion termini of the 14 defective prophages coincided in five out of nine regions separating the nine deletion segments. Various possible explanations are discussed for the nonrandom recurrence of these deletion termini, including the evidence of hot spots of recombination.", "contents": "Genetic studies of coliphage P1. I. Mapping by use of prophage deletions. One hundred and ten amber mutants of coliphage P1 were isolated and localized into groups with respect to the existing genetic map by use of nonpermissive Escherichia coli K-12 strains lysogenic for P1 with deletions. These lysogens contain one of three types of deletion prophages: P1cry and its derivatives, P1dlacs, and P1dpros. Fourteen such lysogens were tested for their ability to rescue the amber mutants which were then assigned to one of nine deletion segments of the P1 genome defined by the termini of the various prophage deletions. The relationship of the nine deletion segments with the published P1 map is described, two new segments having been added. The deletions of the 14 prophages overlapped sufficiently to indicate that the P1 genetic prophage map should be represented in circular form, which is consistent with the fact that P1 is normally a circular plasmid in the prophage state. The distribution of mutants into deletion segments is nonrandom for at least one segment. In addition, the deletion termini of the 14 defective prophages coincided in five out of nine regions separating the nine deletion segments. Various possible explanations are discussed for the nonrandom recurrence of these deletion termini, including the evidence of hot spots of recombination."}
{"id": "PMID:1099232", "title": "Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant.", "content": "The Escherichia coli dnaC protein is not absolutely required in vivo for bacteriophage phiX174 parental replicative-form synthesis (Kranias and Dumas, 1974). However, when rifampin is present at a concentration that inhibits DNA-dependent RNA polymerase, phiX174 parental replicative-form synthesis is dependent on the dnaC protein activity. We conclude that E. coli DNA-dependent RNA polymerase can substitute for the dnaC protein in phiX174 parental replicative-form DNA synthesis, presumably in its initiation. The implications of this result with respect to the in vitro synthesis of the complementary strand of phiX174 DNA are discussed.", "contents": "Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant. The Escherichia coli dnaC protein is not absolutely required in vivo for bacteriophage phiX174 parental replicative-form synthesis (Kranias and Dumas, 1974). However, when rifampin is present at a concentration that inhibits DNA-dependent RNA polymerase, phiX174 parental replicative-form synthesis is dependent on the dnaC protein activity. We conclude that E. coli DNA-dependent RNA polymerase can substitute for the dnaC protein in phiX174 parental replicative-form DNA synthesis, presumably in its initiation. The implications of this result with respect to the in vitro synthesis of the complementary strand of phiX174 DNA are discussed."}
{"id": "PMID:1099233", "title": "Escherichia coli capsule bacteriophages. VII. Bacteriophage 29-host capsular polysaccharide interactions.", "content": "Different interactions between particles of Escherichia coli capsule bacteriophage 29 and its receptor, the E. coli serotype 29 capsular polysaccharide have been studied. The inactivation of phage 29 (8 x 10(3) PFU/ml) by isolated host capsular glycan was found to be physiologically insignificant (50% inactivation dose equals 100 mug after 1 h at 37 C). No adsorption (less than 2 x 10(4) PFU/mug) of the viruses to K29 polysaccharide-coated erythroyctes (at 0 or 37 C) was observed either. The phage particles were, however, found to catalyze the hydrolysis of beta-D-glucosido-(1leads to 3)-D-glucuronic acid bonds (arrow) in the receptor polymer, leading, ultimately, to the formation of a mixture of K29 hexasaccharide (one repeating unit), dodecasaccharide, and octadecasaccharide: (see article). Testing derivatives of K29 polysaccharide, as well as 82 heterologous bacterial (mainly Enteriobactericeae) capsular glycans, the viral glycanase was found to be highly specific; in accordance with the host range of phage 29, only one enzymatic cross-reaction (with the Klebsiella K31 polysaccharide) was observed. These and previous results, as well as the electron optical findings of M. E. Bayer and H. Thurow (submitted for publication), are discussed in terms of a unifying mechanism of phage 29-host capsule interaction. We propose that the viruses penetrate the capsules by means of their spike-associated glycanase activity, which leads them along capsular polysaccharide strands to membrane-cell wall adhesions where ejection of the viral genomes occurs.", "contents": "Escherichia coli capsule bacteriophages. VII. Bacteriophage 29-host capsular polysaccharide interactions. Different interactions between particles of Escherichia coli capsule bacteriophage 29 and its receptor, the E. coli serotype 29 capsular polysaccharide have been studied. The inactivation of phage 29 (8 x 10(3) PFU/ml) by isolated host capsular glycan was found to be physiologically insignificant (50% inactivation dose equals 100 mug after 1 h at 37 C). No adsorption (less than 2 x 10(4) PFU/mug) of the viruses to K29 polysaccharide-coated erythroyctes (at 0 or 37 C) was observed either. The phage particles were, however, found to catalyze the hydrolysis of beta-D-glucosido-(1leads to 3)-D-glucuronic acid bonds (arrow) in the receptor polymer, leading, ultimately, to the formation of a mixture of K29 hexasaccharide (one repeating unit), dodecasaccharide, and octadecasaccharide: (see article). Testing derivatives of K29 polysaccharide, as well as 82 heterologous bacterial (mainly Enteriobactericeae) capsular glycans, the viral glycanase was found to be highly specific; in accordance with the host range of phage 29, only one enzymatic cross-reaction (with the Klebsiella K31 polysaccharide) was observed. These and previous results, as well as the electron optical findings of M. E. Bayer and H. Thurow (submitted for publication), are discussed in terms of a unifying mechanism of phage 29-host capsule interaction. We propose that the viruses penetrate the capsules by means of their spike-associated glycanase activity, which leads them along capsular polysaccharide strands to membrane-cell wall adhesions where ejection of the viral genomes occurs."}
{"id": "PMID:1099234", "title": "Identification of bacteriophage T4-specific precursor tRNA by using a host mutant defective in the methylation of tRNA.", "content": "A mutant of Escherichia coli K-12 that is defective in the synthesis of 5-methyluridine (ribothymidine) in tRNA was used to identify precursors to phage T4-specific tRNA. The precursor molecules, isolated by gel filtration, were more than twice the size of tRNA. This method is suitable for isolation of rather large amounts of such precursor molecules.", "contents": "Identification of bacteriophage T4-specific precursor tRNA by using a host mutant defective in the methylation of tRNA. A mutant of Escherichia coli K-12 that is defective in the synthesis of 5-methyluridine (ribothymidine) in tRNA was used to identify precursors to phage T4-specific tRNA. The precursor molecules, isolated by gel filtration, were more than twice the size of tRNA. This method is suitable for isolation of rather large amounts of such precursor molecules."}
{"id": "PMID:1099235", "title": "Ureteroneocystostomy versus ureteropyelostomy: a comparison in the same renal allograft series.", "content": "Of the 132 transplants in our series 44 patients underwent reconstruction by ureteropyelostomy (group 1) and 88 underwent reconstruction by ureteroneocystostomy (group 2). The rates of major urologic complications were 11 percent in group 1 and 4 per cent in group 2. These results support the widely held opinion that ureteroneocystostomy is the superior method for urinary reconstruction in renal allograft cases. However, the alternative of ureteropyelostomy should be part of every transplant surgeons' armamentarium, especially for cadaver transplants when ureteral blood supply may be compromised.", "contents": "Ureteroneocystostomy versus ureteropyelostomy: a comparison in the same renal allograft series. Of the 132 transplants in our series 44 patients underwent reconstruction by ureteropyelostomy (group 1) and 88 underwent reconstruction by ureteroneocystostomy (group 2). The rates of major urologic complications were 11 percent in group 1 and 4 per cent in group 2. These results support the widely held opinion that ureteroneocystostomy is the superior method for urinary reconstruction in renal allograft cases. However, the alternative of ureteropyelostomy should be part of every transplant surgeons' armamentarium, especially for cadaver transplants when ureteral blood supply may be compromised."}
{"id": "PMID:1099258", "title": "Limulus lysate test for gram-negative bacterial meningitis. Bedside application.", "content": "The limulus lysate on cerebrospinal fluid was evaluated in 335 infants and children as a method for the rapid diagnosis of Gram-negative bacterial meningitis. Positive limulus tests were obtained within one hour in 33 of 34 cases of Hemophilus influenzae meningitis; four additional patients with Gram-negative meningitis also showed positive limulus lysate tests. Conversely, 13 patients with Gram-positive bacterial meningitis all yielded negative limulus assays. All 48 cases of aseptic meningitis and 236 children with no meningitis showed negative limulus assays. Antibiotic therapy prior to hospitalization did not vitiate the validity of the test. A bedside adaptation of the limulus test, performed by house officers and medical students, showed approximately 98% agreement with the laboratory assay.", "contents": "Limulus lysate test for gram-negative bacterial meningitis. Bedside application. The limulus lysate on cerebrospinal fluid was evaluated in 335 infants and children as a method for the rapid diagnosis of Gram-negative bacterial meningitis. Positive limulus tests were obtained within one hour in 33 of 34 cases of Hemophilus influenzae meningitis; four additional patients with Gram-negative meningitis also showed positive limulus lysate tests. Conversely, 13 patients with Gram-positive bacterial meningitis all yielded negative limulus assays. All 48 cases of aseptic meningitis and 236 children with no meningitis showed negative limulus assays. Antibiotic therapy prior to hospitalization did not vitiate the validity of the test. A bedside adaptation of the limulus test, performed by house officers and medical students, showed approximately 98% agreement with the laboratory assay."}
{"id": "PMID:1099261", "title": "The Johns Hopkins rechargeable pacemaker. Historical aspect.", "content": "Several groups have attempted to develop a clinically usable rechargeable cardiac pacemaker. Because of the technical problems, of the 11 reported efforts, only one has met with success, the unit developed at the Johns Hopkins University. A brief review of rechargeable pacemakers is given, along with a discussion of technical problems that required solution before the rechargeable pacemaker could be used clinically. The rechargeable pacemaker, because of its size, should minimize patient problems of discomfort, cosmesis, and skin erosion. The anticipated life of the rechargeable power cell should eliminate the need for pulse generator replacement during the patients's lifetime.", "contents": "The Johns Hopkins rechargeable pacemaker. Historical aspect. Several groups have attempted to develop a clinically usable rechargeable cardiac pacemaker. Because of the technical problems, of the 11 reported efforts, only one has met with success, the unit developed at the Johns Hopkins University. A brief review of rechargeable pacemakers is given, along with a discussion of technical problems that required solution before the rechargeable pacemaker could be used clinically. The rechargeable pacemaker, because of its size, should minimize patient problems of discomfort, cosmesis, and skin erosion. The anticipated life of the rechargeable power cell should eliminate the need for pulse generator replacement during the patients's lifetime."}
{"id": "PMID:1099263", "title": "Exercise-induced asthma. Pharmacological assessment of single drugs and drug combinations.", "content": "The relative effectiveness of ephedrine, theophylline, and hydroxyzine hydrochloride and their combinations was studied in a two-part investigation of 16 youths who has asthma and exercise-induced asthma. The beneficial and adverse effects of the drugs in the control of asthma were studied in the first part of the investigation. Both theophylline and ephedrine were judged superior to placebo when given alone. When combined they were somewhat more effective than either agent alone, although adverse effects precluded their use in almost half of the subjects. The addition of hydroxyzine diminished the side effects sufficiently to make the combination acceptable to four subjects who refused to take ephedrine/theophylline. In the second part of the study, the relief of exercise-induced asthma was evaluated. Ephedrine had no effect, hydroxyzine had a weak effect on hastening recovery, but theophylline modified the postexercise response significantly. The three drugs together produced an additive effect superior to that of theophylline alone.", "contents": "Exercise-induced asthma. Pharmacological assessment of single drugs and drug combinations. The relative effectiveness of ephedrine, theophylline, and hydroxyzine hydrochloride and their combinations was studied in a two-part investigation of 16 youths who has asthma and exercise-induced asthma. The beneficial and adverse effects of the drugs in the control of asthma were studied in the first part of the investigation. Both theophylline and ephedrine were judged superior to placebo when given alone. When combined they were somewhat more effective than either agent alone, although adverse effects precluded their use in almost half of the subjects. The addition of hydroxyzine diminished the side effects sufficiently to make the combination acceptable to four subjects who refused to take ephedrine/theophylline. In the second part of the study, the relief of exercise-induced asthma was evaluated. Ephedrine had no effect, hydroxyzine had a weak effect on hastening recovery, but theophylline modified the postexercise response significantly. The three drugs together produced an additive effect superior to that of theophylline alone."}
{"id": "PMID:1099269", "title": "[Transmission of drug-resistance through conjugation in Yersinia enterocolitica and Yersinia pseudotuberculosis. (II). With special reference to streptomycin resistant strains isolated from men and domestic animals (author's transl)].", "content": "Seven strains of Y. enterocolitica, of which 2 strains being resistant to SM, TC and SA, 2 to SM and SA 3 to SM, and 3 strains of Y. pseudotuberculosis resistant to SM have been isolated from men and domestic animals. Tests were carried out with these 10 isolates for transfer of the drug resistance by bacterial conjugation, and evidence was obtained for transfer of resistance from 1 SM-resistant Y. enterocolitica and 2 Y. pseudotuberculosis strains to the sensitive E. coli K-12. Moreover, secondary transfer of this resistance to sensitive strains of the donor species Y. enterocolitica and Y pseudotuberculosis was also demonstrated. The findings indicate the spontaneous occurrence of organisms carrying the resistance transfer factor, R(Sm), among the populations of these two species in the natural environment.", "contents": "[Transmission of drug-resistance through conjugation in Yersinia enterocolitica and Yersinia pseudotuberculosis. (II). With special reference to streptomycin resistant strains isolated from men and domestic animals (author's transl)]. Seven strains of Y. enterocolitica, of which 2 strains being resistant to SM, TC and SA, 2 to SM and SA 3 to SM, and 3 strains of Y. pseudotuberculosis resistant to SM have been isolated from men and domestic animals. Tests were carried out with these 10 isolates for transfer of the drug resistance by bacterial conjugation, and evidence was obtained for transfer of resistance from 1 SM-resistant Y. enterocolitica and 2 Y. pseudotuberculosis strains to the sensitive E. coli K-12. Moreover, secondary transfer of this resistance to sensitive strains of the donor species Y. enterocolitica and Y pseudotuberculosis was also demonstrated. The findings indicate the spontaneous occurrence of organisms carrying the resistance transfer factor, R(Sm), among the populations of these two species in the natural environment."}
{"id": "PMID:1099271", "title": "[The characteristics of the antibacterial activity of garlic (author's transl)].", "content": "It has been found by Cavallito (1944) that garlic (Allium sativum L.) has a strong antibacterial activity and the active principle of garlic is named \"Allicin\" and it has been confirmed by Stoll et al. (1948) that the allicin is derived from the alliin-alliinase system. Nevertheless, one worker in Japan (1951) stands on another view point which the effective component of garlic is some sugar-proteim because he could not find alliin in the native garlic. We intend to make sure that the alliin is contained in the native garlic, by using Hitachi Amino acid Analyser with Fraction Collecter and compared with garlic of other countrys' production. As the result of examinations, we found that the good amounts of alliin is contained in the native garlic also. The contents of alliin in garlic is varied by the species, the cultivation mode, and the district of production. The form of alliin in the tissue is a free style, and thereupon it is easy to change its chemical conformation by the method of extraction. We compared the relative capacity between 1 allicin unit by Klein (1954) and 1 cup unit by Schmidt et al. (1947). Furthermore we tested the antibiotic spectrum, using the pure crystallized alliin with the sufficient amount of raw alliinase gained by the isoelectric point precipitation and checked upon wide spectrum for Mycobacterium tuberculosis, Gram-positive and negative bacteria, and some fungi.", "contents": "[The characteristics of the antibacterial activity of garlic (author's transl)]. It has been found by Cavallito (1944) that garlic (Allium sativum L.) has a strong antibacterial activity and the active principle of garlic is named \"Allicin\" and it has been confirmed by Stoll et al. (1948) that the allicin is derived from the alliin-alliinase system. Nevertheless, one worker in Japan (1951) stands on another view point which the effective component of garlic is some sugar-proteim because he could not find alliin in the native garlic. We intend to make sure that the alliin is contained in the native garlic, by using Hitachi Amino acid Analyser with Fraction Collecter and compared with garlic of other countrys' production. As the result of examinations, we found that the good amounts of alliin is contained in the native garlic also. The contents of alliin in garlic is varied by the species, the cultivation mode, and the district of production. The form of alliin in the tissue is a free style, and thereupon it is easy to change its chemical conformation by the method of extraction. We compared the relative capacity between 1 allicin unit by Klein (1954) and 1 cup unit by Schmidt et al. (1947). Furthermore we tested the antibiotic spectrum, using the pure crystallized alliin with the sufficient amount of raw alliinase gained by the isoelectric point precipitation and checked upon wide spectrum for Mycobacterium tuberculosis, Gram-positive and negative bacteria, and some fungi."}
{"id": "PMID:1099274", "title": "Multiclinical open studies on the effect of beta-methyldigoxin on congestive heart failure with atrial fibrillation.", "content": "Clinical open trials of beta-methyldigoxin were carried out in 15 institutions in order to examine the effect, usefulness and ease of its oral administration. In the case of oral digitalization with 0.2 mg, 3 times daily, an effect was obtained in all of 13 cases of congestive heart failure accompanied by atrial fibrillation or flutter. The average time and dose required for digitalization were about 50 hours and 1.27 mg respectively. In 9 of the 13 cases, the effect was achieved within 48 hours. The average maintenance does of beta-methyldigoxin in 102 cases of congestive heart failure with atrial fibrillation was 0.177 mg per day. About 75% of the cases were maintained with 0.15 to 0.2 mg. This range of dose of beta-methyldigoxin was much smaller than that of digoxin in our series. This can be ascribed to a higher absorption rate of beta-methyldigoxin from the digestive tract. Studies on the cases in which patients previously treated with other glycosides were switched over to beta-methyldigoxin revealed that 1 mg of beta-methyldigoxin is equivalent to 1.8 mg of digoxin or to 0.59 mg of digitoxin. The usefulness and ease of beta-methyldigoxin in maintenance was evaluated as being somewhat superior to other cardiac glycosides, according to the global judgement of the physicians. The observed side effects were similar to those of other glycosides in frequency and character.", "contents": "Multiclinical open studies on the effect of beta-methyldigoxin on congestive heart failure with atrial fibrillation. Clinical open trials of beta-methyldigoxin were carried out in 15 institutions in order to examine the effect, usefulness and ease of its oral administration. In the case of oral digitalization with 0.2 mg, 3 times daily, an effect was obtained in all of 13 cases of congestive heart failure accompanied by atrial fibrillation or flutter. The average time and dose required for digitalization were about 50 hours and 1.27 mg respectively. In 9 of the 13 cases, the effect was achieved within 48 hours. The average maintenance does of beta-methyldigoxin in 102 cases of congestive heart failure with atrial fibrillation was 0.177 mg per day. About 75% of the cases were maintained with 0.15 to 0.2 mg. This range of dose of beta-methyldigoxin was much smaller than that of digoxin in our series. This can be ascribed to a higher absorption rate of beta-methyldigoxin from the digestive tract. Studies on the cases in which patients previously treated with other glycosides were switched over to beta-methyldigoxin revealed that 1 mg of beta-methyldigoxin is equivalent to 1.8 mg of digoxin or to 0.59 mg of digitoxin. The usefulness and ease of beta-methyldigoxin in maintenance was evaluated as being somewhat superior to other cardiac glycosides, according to the global judgement of the physicians. The observed side effects were similar to those of other glycosides in frequency and character."}
{"id": "PMID:1099286", "title": "Microbial adjuvant and autoimmunity. I. Induction of antibody responses to syngeneic tissue extracts in mice treated with capsular polysaccharide of Klebsiella pneumoniae.", "content": "With the use of the capsular polysaccharide of Klebsiella pneumoniae (CPS-K) as a powerful adjuvant, high precipitin responses could be induced in mice to syngeneic eyeball extracts and thyroid gland extracts which were normally nonimmunogenic. Only very weak responses were induced to eyeball extracts by Freund's complete adjuvant. Repeated administrations of the antigens mixed with CPS-K at time intervals of 30 days (more than twice for the eyeballs or more than three times for the thyroid glands) were required for induction of high precipitin responses. Antibody responses detectable by the immunofluorescent technique could be induced to syngeneic lymphoid tissue extracts by injecting the mixture of antigen and CPS-K more than five times at time intervals of 30 days. These findings suggest that repeated stimulation by autoantigens together with such a strong adjuvant as CPS-K can terminate natural tolerance against autoantigens.", "contents": "Microbial adjuvant and autoimmunity. I. Induction of antibody responses to syngeneic tissue extracts in mice treated with capsular polysaccharide of Klebsiella pneumoniae. With the use of the capsular polysaccharide of Klebsiella pneumoniae (CPS-K) as a powerful adjuvant, high precipitin responses could be induced in mice to syngeneic eyeball extracts and thyroid gland extracts which were normally nonimmunogenic. Only very weak responses were induced to eyeball extracts by Freund's complete adjuvant. Repeated administrations of the antigens mixed with CPS-K at time intervals of 30 days (more than twice for the eyeballs or more than three times for the thyroid glands) were required for induction of high precipitin responses. Antibody responses detectable by the immunofluorescent technique could be induced to syngeneic lymphoid tissue extracts by injecting the mixture of antigen and CPS-K more than five times at time intervals of 30 days. These findings suggest that repeated stimulation by autoantigens together with such a strong adjuvant as CPS-K can terminate natural tolerance against autoantigens."}
{"id": "PMID:1099287", "title": "Arginine gene cluster of Serratia marcescens.", "content": "Biochemical and genetic studies on the arginine-requiring auxotrophs derived from a Serratia marcescens strain were carried out. The arg mutants were classified into seven biochemical groups based on their growth response to five precursors of arginine biosynthesis and enzyme deficiency. Reciprocal transduction tests among these arg mutants divided them into three linkage groups, and the fine mapping in each of the groups by two- or three-point crosses revealed the following arrangement of loci. (1) arg44-thy11-lys1; (2) met1-glt2-argE-(arg19-arg51)-arg120-argG-argH; (3) arg33-pyr4. Five of the seven biochemically distinct arg mutants belonged to the second linkage group, and they constituted an agr-gene cluster. A characteristic feature of the arg-gene cluster of S. marcescens is that it involves argG, which was previously reported only in the Proteus group of Enterobacteriaceae.", "contents": "Arginine gene cluster of Serratia marcescens. Biochemical and genetic studies on the arginine-requiring auxotrophs derived from a Serratia marcescens strain were carried out. The arg mutants were classified into seven biochemical groups based on their growth response to five precursors of arginine biosynthesis and enzyme deficiency. Reciprocal transduction tests among these arg mutants divided them into three linkage groups, and the fine mapping in each of the groups by two- or three-point crosses revealed the following arrangement of loci. (1) arg44-thy11-lys1; (2) met1-glt2-argE-(arg19-arg51)-arg120-argG-argH; (3) arg33-pyr4. Five of the seven biochemically distinct arg mutants belonged to the second linkage group, and they constituted an agr-gene cluster. A characteristic feature of the arg-gene cluster of S. marcescens is that it involves argG, which was previously reported only in the Proteus group of Enterobacteriaceae."}
{"id": "PMID:1099288", "title": "Chemical studies on the cell walls of Leptorspira biflexa strain Urawa and Treponema pallidum strain Reiter.", "content": "The preparation and chemical properties of the cell walls of Leptospira biflexa Urawa and Treponema pallidum Reiter are described. Both cell walls are composed mainly of polysaccharides and peptidoglycans. The data of chemical analysis indicate that the cell wall of L. biflexa Urawa contains rhamnose, arabinose, xylose, mannose, galactose, glucose and unidentified sugars as neutral sugars, and alanine, glutamic acid, alpha, epsilon-diaminopimelic acid, glucosamine and muramic acid as major amino acids and amino sugars. As major chemical constituents of the cell wall of T. pallidum Reiter, rhamnose, arabinose, xylose, mannose, galactose, glucose, alanine, glutamic acid, ornithine, glycine, glucosamine and muramic acid have been detected. The chemical properties of protein and polysaccharide fractions prepared from the cells of T. pallidum Reiter were also partially examined.", "contents": "Chemical studies on the cell walls of Leptorspira biflexa strain Urawa and Treponema pallidum strain Reiter. The preparation and chemical properties of the cell walls of Leptospira biflexa Urawa and Treponema pallidum Reiter are described. Both cell walls are composed mainly of polysaccharides and peptidoglycans. The data of chemical analysis indicate that the cell wall of L. biflexa Urawa contains rhamnose, arabinose, xylose, mannose, galactose, glucose and unidentified sugars as neutral sugars, and alanine, glutamic acid, alpha, epsilon-diaminopimelic acid, glucosamine and muramic acid as major amino acids and amino sugars. As major chemical constituents of the cell wall of T. pallidum Reiter, rhamnose, arabinose, xylose, mannose, galactose, glucose, alanine, glutamic acid, ornithine, glycine, glucosamine and muramic acid have been detected. The chemical properties of protein and polysaccharide fractions prepared from the cells of T. pallidum Reiter were also partially examined."}
{"id": "PMID:1099296", "title": "Acquiescent renal infection.", "content": "The relationship between bacterial infection of the renal parenchyma with Escherichia coli and the establishment of pathologic lesions has been investigate experimentally.  Infection was established in one kidney and the bacteriologic, pathologic and immunologic features of infection were compared in the pyelonephritic and contralateral unmanipulated kidney.  Whereas active bacterial infection was associated with pathologic changes in the pyelonephritic kidney, a poor correlation was found between bacterial growth and the gross pathology and histopathologic changes in the contralateral kidney.  The conclusion from these studies is that infection of the kidney is not always associated with pathologic changes.  The term \"acquiescent infection\" has been used to describe this host-parasite relationship in which active, persistent, bacterial infection is not associated with pathologic lesions.  Evidence is presented that bacteria in the contralateral unmanipulated kidney are present in the renal parenchyma and that bacterial proliferation can be induced following renal trauma.  Activation of infection and bacterial proliferation did not always result in histopathologic damage to the kidney and was not associated with an increase in serum antibody.", "contents": "Acquiescent renal infection. The relationship between bacterial infection of the renal parenchyma with Escherichia coli and the establishment of pathologic lesions has been investigate experimentally.  Infection was established in one kidney and the bacteriologic, pathologic and immunologic features of infection were compared in the pyelonephritic and contralateral unmanipulated kidney.  Whereas active bacterial infection was associated with pathologic changes in the pyelonephritic kidney, a poor correlation was found between bacterial growth and the gross pathology and histopathologic changes in the contralateral kidney.  The conclusion from these studies is that infection of the kidney is not always associated with pathologic changes.  The term \"acquiescent infection\" has been used to describe this host-parasite relationship in which active, persistent, bacterial infection is not associated with pathologic lesions.  Evidence is presented that bacteria in the contralateral unmanipulated kidney are present in the renal parenchyma and that bacterial proliferation can be induced following renal trauma.  Activation of infection and bacterial proliferation did not always result in histopathologic damage to the kidney and was not associated with an increase in serum antibody."}
{"id": "PMID:1099301", "title": "Cardiovascular disease in uremic patients on hemodialysis.", "content": "In conclusion, patients on chronic maintenance dialysis have an increased incidence of death from cardiovascular disease. Hypertension plays a major role, and these patients must be carefully monitored for complete control of blood pressure. Adequacy of ultrafiltration to maintain normal extracellular volume is an essential part of the dialytic treatment. Hypertensive patients should be screened for excessive renin secretion because of its possible role in unresponsive hypertension in patients on dialysis. Nephrectomy should be used when necessary, where dialysis and antihypertensive medication have not adequately controlled blood pressure. Patients must be monitored for the presence of pericardial disease to avoid subsequent pericardial effusion and the development of constrictive pericarditis with its adverse effect on myocardial function. When constrictive pericarditis is present, it obviously should be relieved by appropriate surgery. Efforts should be made to minimize cardiac output in hemodialysis patients. Whether or not routine transfusions to maintain a higher hematocrit are indicated is a question that cannot yet be answered. However, patients with marginal cardiovascular function who are accepted on hemodialysis and must have an arteriovenous shunt should be supported in any manner to minimize an increase in cardiac output. Early and aggressive treatment of known episodes of sepsis is important in the elimination of valvular endocarditis in this patient population. Perhaps one of the finer indicators of adequacy of hemodialysis will be K rate and peak immunoreactive insulin levels. Continued abnormality of these parameters may contribute to cardiovascular disease. Clearly, further study of the effect of abnormal carbohydrate metabolism on lipid metabolism is in order. Serum triglyceride, serum cholesterol and lipid electrophoretic pattern should be followed to evaluate the beneficial effects of drug therapy and changes in dialytic technique on the development of cardiovascular disease. Careful monitoring of calcium, phosphorus, bone films and parathyroid hormone levels is indicated to assess parathyroid status. The use of aluminum binders and parathyroidectomy to prevent vascular and myocardial calcification is important in the therapy of these patients. The use of cardiac catheterization, coronary artery arteriography, and possibly cardiac vascular repair, should be considered in the chronic hemodialysis patient with coronary artery disease if he is otherwise well. Adequacy of hemodialysis perhaps can be evaluated through its effect on all of the above parameters. Whether or not changes in artificial kidney treatments can correct the final vascular disease remains to be seen.", "contents": "Cardiovascular disease in uremic patients on hemodialysis. In conclusion, patients on chronic maintenance dialysis have an increased incidence of death from cardiovascular disease. Hypertension plays a major role, and these patients must be carefully monitored for complete control of blood pressure. Adequacy of ultrafiltration to maintain normal extracellular volume is an essential part of the dialytic treatment. Hypertensive patients should be screened for excessive renin secretion because of its possible role in unresponsive hypertension in patients on dialysis. Nephrectomy should be used when necessary, where dialysis and antihypertensive medication have not adequately controlled blood pressure. Patients must be monitored for the presence of pericardial disease to avoid subsequent pericardial effusion and the development of constrictive pericarditis with its adverse effect on myocardial function. When constrictive pericarditis is present, it obviously should be relieved by appropriate surgery. Efforts should be made to minimize cardiac output in hemodialysis patients. Whether or not routine transfusions to maintain a higher hematocrit are indicated is a question that cannot yet be answered. However, patients with marginal cardiovascular function who are accepted on hemodialysis and must have an arteriovenous shunt should be supported in any manner to minimize an increase in cardiac output. Early and aggressive treatment of known episodes of sepsis is important in the elimination of valvular endocarditis in this patient population. Perhaps one of the finer indicators of adequacy of hemodialysis will be K rate and peak immunoreactive insulin levels. Continued abnormality of these parameters may contribute to cardiovascular disease. Clearly, further study of the effect of abnormal carbohydrate metabolism on lipid metabolism is in order. Serum triglyceride, serum cholesterol and lipid electrophoretic pattern should be followed to evaluate the beneficial effects of drug therapy and changes in dialytic technique on the development of cardiovascular disease. Careful monitoring of calcium, phosphorus, bone films and parathyroid hormone levels is indicated to assess parathyroid status. The use of aluminum binders and parathyroidectomy to prevent vascular and myocardial calcification is important in the therapy of these patients. The use of cardiac catheterization, coronary artery arteriography, and possibly cardiac vascular repair, should be considered in the chronic hemodialysis patient with coronary artery disease if he is otherwise well. Adequacy of hemodialysis perhaps can be evaluated through its effect on all of the above parameters. Whether or not changes in artificial kidney treatments can correct the final vascular disease remains to be seen."}
{"id": "PMID:1099335", "title": "Immunosuppression in rabbits: skin and kidney allografts.", "content": "Both skin and kidney allografts were at least partially protected from rejection by daily treatment with 1-0 mg/kg of prednisolone acetate. Azathioprine, cyclophosphamide, antilymphocytic serum, and attempts to produce a tolerant state with prior grafting of specific donor antigen, all failed to alter the course of rejection.", "contents": "Immunosuppression in rabbits: skin and kidney allografts. Both skin and kidney allografts were at least partially protected from rejection by daily treatment with 1-0 mg/kg of prednisolone acetate. Azathioprine, cyclophosphamide, antilymphocytic serum, and attempts to produce a tolerant state with prior grafting of specific donor antigen, all failed to alter the course of rejection."}
{"id": "PMID:1099336", "title": "Endothelial injury induced by thrombin or thrombi.", "content": "A variety of blood constituents was injected into an isolated segment of rabbit aorta to determine which elements might be involved in early endothelial injury. Test materials consisted of platelet-rich plasma (PRP) alone; PRP plus adenosine diphosphate (ADP); PRP plus tendon extract; PRP plus thrombin; ultrasonicated PRP alone; platelet-poor plasma alone; and thrombin in saline. Each experimental mixture was left in the aorta for 15 minutes, followed by reflow for 20 minutes. The vessel was then fixed by glutaraldehyde perfusion. Thick sections of the entire circumference of the aorta were taken for phase contrast microscopy and representative arease were selected for electron microscopy. In control PRP alone, platelet-poor plasma alone and with PRP plus ADP there were occasional subendothelial vesicles. When PRP plus thrombin and platelet-poor plasma plus thrombin were injected separately to form a thrombus or when thrombin in saline was used, there was extensive subendothelial vesiculation with focal ulceration and adherence of thrombus to endothelium. Severe injury was associated with the presence of thrombin initiating the polymerization of fibrinogen to fibrin. Electron micrographs demonstrate the earliest lesion as a disruption of the superficial fibrilliary elastica with separation of overlying endothelium.", "contents": "Endothelial injury induced by thrombin or thrombi. A variety of blood constituents was injected into an isolated segment of rabbit aorta to determine which elements might be involved in early endothelial injury. Test materials consisted of platelet-rich plasma (PRP) alone; PRP plus adenosine diphosphate (ADP); PRP plus tendon extract; PRP plus thrombin; ultrasonicated PRP alone; platelet-poor plasma alone; and thrombin in saline. Each experimental mixture was left in the aorta for 15 minutes, followed by reflow for 20 minutes. The vessel was then fixed by glutaraldehyde perfusion. Thick sections of the entire circumference of the aorta were taken for phase contrast microscopy and representative arease were selected for electron microscopy. In control PRP alone, platelet-poor plasma alone and with PRP plus ADP there were occasional subendothelial vesicles. When PRP plus thrombin and platelet-poor plasma plus thrombin were injected separately to form a thrombus or when thrombin in saline was used, there was extensive subendothelial vesiculation with focal ulceration and adherence of thrombus to endothelium. Severe injury was associated with the presence of thrombin initiating the polymerization of fibrinogen to fibrin. Electron micrographs demonstrate the earliest lesion as a disruption of the superficial fibrilliary elastica with separation of overlying endothelium."}
{"id": "PMID:1099337", "title": "A controlled sequential morphologic study of hyperacute cardiac allograft rejection in the rat.", "content": "A series of heterotopic cardiac allografts from ACI strain rat donors to ACI skin-presensitized Lewis strain recipients was examined by light and electron microscopy at intervals from 1 minute to 24 hours for a sequential morphologic study of hyperacute rejection. Syngeneic Lewis hearts grafts in ACI skin-presensitized Lewis recipients provided controls. Extensive platelet aggregation throughout the vasculature of the allografts in the presence of a largely intact endothelium was the initial morphologic event, which was present by 1 minute posttransplant and absent in all syngeneic controls. This was followed by widespread endothelial damage. Endothelial damage preceded neutrophil margination and emigration in the graft. Neutrophil degranulation or evidence of phagocytosis was not observed within the 1st hour. At 6 and 24 hours, degranulated forms were common and apparent extrusion of granule contents with progressive ischemic injury. Segmental occlusion of the vasculature by platelet plugs and progressive disruption and loss of capillaries combined with an increase in permeability may result in myocardial ischemia. Neutrophil-derived agents do not appear to be of major significance in hyperacute rejection during the early posttransplant phase, but may play a role in mediating myocardial injury if the allograft survives for alonger period. However, on morphologic grounds, it is suggested that microcirculatory hypoperfusion with resultant ischemia is a major determinant of myocardial damage and graft function. No significant change was noted over the observation period in peripheral neutrophil or platelet counts when compared to syngeneic controls, and a slight reudction in specific cytotoxic antibody titer in the allografts was immediate but very transient.", "contents": "A controlled sequential morphologic study of hyperacute cardiac allograft rejection in the rat. A series of heterotopic cardiac allografts from ACI strain rat donors to ACI skin-presensitized Lewis strain recipients was examined by light and electron microscopy at intervals from 1 minute to 24 hours for a sequential morphologic study of hyperacute rejection. Syngeneic Lewis hearts grafts in ACI skin-presensitized Lewis recipients provided controls. Extensive platelet aggregation throughout the vasculature of the allografts in the presence of a largely intact endothelium was the initial morphologic event, which was present by 1 minute posttransplant and absent in all syngeneic controls. This was followed by widespread endothelial damage. Endothelial damage preceded neutrophil margination and emigration in the graft. Neutrophil degranulation or evidence of phagocytosis was not observed within the 1st hour. At 6 and 24 hours, degranulated forms were common and apparent extrusion of granule contents with progressive ischemic injury. Segmental occlusion of the vasculature by platelet plugs and progressive disruption and loss of capillaries combined with an increase in permeability may result in myocardial ischemia. Neutrophil-derived agents do not appear to be of major significance in hyperacute rejection during the early posttransplant phase, but may play a role in mediating myocardial injury if the allograft survives for alonger period. However, on morphologic grounds, it is suggested that microcirculatory hypoperfusion with resultant ischemia is a major determinant of myocardial damage and graft function. No significant change was noted over the observation period in peripheral neutrophil or platelet counts when compared to syngeneic controls, and a slight reudction in specific cytotoxic antibody titer in the allografts was immediate but very transient."}
{"id": "PMID:1099338", "title": "Perinatal reactions of the rabbit lung to maternal endotoxemia.", "content": "The reactions of the fetal and newborn lung to in utero injury were investigated in rabbits using as a model maternal administration of Escherichia coli endotoxin, 2 to 4 mug per kg. of body weight, injected intravenously at the 29th gestational day. At 6 hours after endotoxin injection, the lung revealed diffuse alveolar damage-sparing type II cells which appeared resistant to injury but more mature than type II cells from control lungs. These changes were followed by a reparative phase which over a 2-day period resulted in increased interstitial cellularity, replacement of the damaged type I cells by proliferated type II cells, and marked intraalveolar accumulation of myelin figures. The above reactions were associated with an average increase of 100 per cent in the phospholipid content of the surfactant fraction isolated from lung washing. Postnatally, the lungs showed structural changes resembling those seen in hyaline membrane disease of the newborn. The findings suggest that the response of the alveolar epithelim to injury inudced late in gestation follows a pattern similar to that described previously in adult lung, and is assoicated with in utero increased release of surfactant. It is proposed that alveolar injury and repair, including reduction of the intracellular surfactant reservoir occurring prior to breathing, may interfere postnatally with formation and maintenance of a normal surfactant layer, thereby predisposing to respiratory distress.", "contents": "Perinatal reactions of the rabbit lung to maternal endotoxemia. The reactions of the fetal and newborn lung to in utero injury were investigated in rabbits using as a model maternal administration of Escherichia coli endotoxin, 2 to 4 mug per kg. of body weight, injected intravenously at the 29th gestational day. At 6 hours after endotoxin injection, the lung revealed diffuse alveolar damage-sparing type II cells which appeared resistant to injury but more mature than type II cells from control lungs. These changes were followed by a reparative phase which over a 2-day period resulted in increased interstitial cellularity, replacement of the damaged type I cells by proliferated type II cells, and marked intraalveolar accumulation of myelin figures. The above reactions were associated with an average increase of 100 per cent in the phospholipid content of the surfactant fraction isolated from lung washing. Postnatally, the lungs showed structural changes resembling those seen in hyaline membrane disease of the newborn. The findings suggest that the response of the alveolar epithelim to injury inudced late in gestation follows a pattern similar to that described previously in adult lung, and is assoicated with in utero increased release of surfactant. It is proposed that alveolar injury and repair, including reduction of the intracellular surfactant reservoir occurring prior to breathing, may interfere postnatally with formation and maintenance of a normal surfactant layer, thereby predisposing to respiratory distress."}
{"id": "PMID:1099339", "title": "Comments on operations for lower limb lymphoedema.", "content": "Good results have been obtained from surgical operations for lymphoedema of the lower limbs using skin flaps with a blood supply to cover the muscles following reduction of swollen subcutaneous tissue. The Charles operation using free skin grafts for cover is reserved for tropical elephantiasis or patients with local skin in bad condition. A variety of other procedures of physiologic intent have given disappointing results and been abandoned. The results of 74 operations are reviewed with a view to improving still further the results. The mortality rate was nil and there were only minor complications.", "contents": "Comments on operations for lower limb lymphoedema. Good results have been obtained from surgical operations for lymphoedema of the lower limbs using skin flaps with a blood supply to cover the muscles following reduction of swollen subcutaneous tissue. The Charles operation using free skin grafts for cover is reserved for tropical elephantiasis or patients with local skin in bad condition. A variety of other procedures of physiologic intent have given disappointing results and been abandoned. The results of 74 operations are reviewed with a view to improving still further the results. The mortality rate was nil and there were only minor complications."}
{"id": "PMID:1099346", "title": "Sliding esophageal hiatal hernia and reflux peptic esophagitis.", "content": "The past two decades have seen outstanding contributions to our knowledge of the physiology of the esophagus, particularly of the lower esophageal sphincter. The clinical syndrome of reflux peptic esophagitis has been clearly delineated and is now well recognized. Although the relationship of the lower esophageal sphincter failure, which causes sliding esophageal hiatal hernia, remains obscure, successful hiatal herniorrhaphy by a variety of methods produces satisfactory clinical results in a majority of patients. There is a significant failure rate in all methods and a morbidity clearly related to operative intervention. Additive surgery such as vagotomy and pyloroplasty is not useful in preventing recurrence and is associated with increased morbidity. Peptic strictures with firm, fibrous stenosis can be satisfactorily treated in most cases with the Thal fundic patch to widen the lumen and Nissen fundoplication to prevent further gastroesophageal reflux.", "contents": "Sliding esophageal hiatal hernia and reflux peptic esophagitis. The past two decades have seen outstanding contributions to our knowledge of the physiology of the esophagus, particularly of the lower esophageal sphincter. The clinical syndrome of reflux peptic esophagitis has been clearly delineated and is now well recognized. Although the relationship of the lower esophageal sphincter failure, which causes sliding esophageal hiatal hernia, remains obscure, successful hiatal herniorrhaphy by a variety of methods produces satisfactory clinical results in a majority of patients. There is a significant failure rate in all methods and a morbidity clearly related to operative intervention. Additive surgery such as vagotomy and pyloroplasty is not useful in preventing recurrence and is associated with increased morbidity. Peptic strictures with firm, fibrous stenosis can be satisfactorily treated in most cases with the Thal fundic patch to widen the lumen and Nissen fundoplication to prevent further gastroesophageal reflux."}
{"id": "PMID:1099347", "title": "Effects of parathyroid hormone on renal function.", "content": "Parathyroid hormone has been recognized as an important factor in the regulation of renal function since the 1920s. Early investigations mainly characterized the role of the hormone in calcium and phosphorus metabolism. Subsequently, significant contributions have been made to the understanding of the influence of parathyroid hormone on acid-base balance and amino acid excretion. The following is a review of the effects of parathyroid hormone in the regulation of renal function with special emphasis on new developments.", "contents": "Effects of parathyroid hormone on renal function. Parathyroid hormone has been recognized as an important factor in the regulation of renal function since the 1920s. Early investigations mainly characterized the role of the hormone in calcium and phosphorus metabolism. Subsequently, significant contributions have been made to the understanding of the influence of parathyroid hormone on acid-base balance and amino acid excretion. The following is a review of the effects of parathyroid hormone in the regulation of renal function with special emphasis on new developments."}
{"id": "PMID:1099348", "title": "The use of computerized transaxial tomography in the diagnosis of tuberous sclerosis.", "content": "Computerized transaxial tomography is a useful test for the recognition of tuberous sclerosis when this diagnosis has not been firmly established by clinical methods. In addition, computerized transaxial tomography may demonstrate the location of obstruction in patients with tuberous sclerosis who have developed signs of increased intracranial presure. These generalizations are supported by evidence from four patients investigated by this test.", "contents": "The use of computerized transaxial tomography in the diagnosis of tuberous sclerosis. Computerized transaxial tomography is a useful test for the recognition of tuberous sclerosis when this diagnosis has not been firmly established by clinical methods. In addition, computerized transaxial tomography may demonstrate the location of obstruction in patients with tuberous sclerosis who have developed signs of increased intracranial presure. These generalizations are supported by evidence from four patients investigated by this test."}
{"id": "PMID:1099355", "title": "Adverse effects of iodides on thyroid function.", "content": "The administration of pharmacologic quantities of iodine such as iodides for the treatment of pulmonary disease, organic iodine present in medications and x-ray contrast dyes, and the ingestion of iodine-rich natural foods, may result in goiter, hypothyroidism, or hyperthyroidism, especially in patients with underlying thyroid disease. Medications containing iodide may induce hypothroidism in euthyroid patients with Hashimoto's thyroiditis, 131I or surgically treated Graves' disease, or following hemithyroidectomy for nodules; and they may induce hyperthyroidism in patients with endemic iodine-deficient goiter, autonomous nodules or nontoxic nodular goiter, or in patients recently treated with antithyroid drugs for Graves' disease. Rarely, hypothyroidism or hyperthyroidism may develop in patients with completely normal thyroid function during administration of iodide. The etiology of iodide-induced goiter and hypothyroidism in patients with cystic fibrosis remains obscure. Iodide-induced myxedema may also occur in patients receiving drugs which alter thyroid function, such as lithium, phenazone, and sulfisoxazole. Finally, iodides do have a role in the treatment of hyperthyroidism but their use should probably be restricted to thyroid storm, preoperative preparation of the hyperthyroid patient, and following 131I treatment.", "contents": "Adverse effects of iodides on thyroid function. The administration of pharmacologic quantities of iodine such as iodides for the treatment of pulmonary disease, organic iodine present in medications and x-ray contrast dyes, and the ingestion of iodine-rich natural foods, may result in goiter, hypothyroidism, or hyperthyroidism, especially in patients with underlying thyroid disease. Medications containing iodide may induce hypothroidism in euthyroid patients with Hashimoto's thyroiditis, 131I or surgically treated Graves' disease, or following hemithyroidectomy for nodules; and they may induce hyperthyroidism in patients with endemic iodine-deficient goiter, autonomous nodules or nontoxic nodular goiter, or in patients recently treated with antithyroid drugs for Graves' disease. Rarely, hypothyroidism or hyperthyroidism may develop in patients with completely normal thyroid function during administration of iodide. The etiology of iodide-induced goiter and hypothyroidism in patients with cystic fibrosis remains obscure. Iodide-induced myxedema may also occur in patients receiving drugs which alter thyroid function, such as lithium, phenazone, and sulfisoxazole. Finally, iodides do have a role in the treatment of hyperthyroidism but their use should probably be restricted to thyroid storm, preoperative preparation of the hyperthyroid patient, and following 131I treatment."}
{"id": "PMID:1099358", "title": "Gonadal steroids and gonadotropins in hyperthyroidism.", "content": "Gynecomastia occurs in about 20 to 40 per cent of men with hyperthyroidism. Serum concentrations of total estradiol-17beta, total testosterone, and luteinizing hormone are supranormal in these patients. Serum concentration of SHBG is also high in hyperthyroidism. This can explain the high serum testosterone, essentially completely but not the high serum estradiol-17beta; thus, whereas serum unbound testosterone is normal, serum unbound estradiol-17beta is above normal in hyperthyroid men. This balance in relative concentrations of unbound gonadal steroids is apparently quite favorable to the development of gynecomastia in hyperthyroidism. Increased peripheral tissue metabolism of androgens to estrogens seems to be the major factor responsible for high estradiol-17beta in hyperthyroidism; increased glandular secretion of estradiol-17beta may also be important. The mechanism of hypomenorrhea or amenorrhea in hyperthyroidism remains unclear. Changes in circulating estrogens, androgens, and  luteinizing hormone in hyperthyroid women are similar to those in hyperthyroid men. The mid-cycle ovulatory peak of luteinizing hormone may be blunted in patients with scanty periods whereas it may be altogether absent in those with amenorrhea.", "contents": "Gonadal steroids and gonadotropins in hyperthyroidism. Gynecomastia occurs in about 20 to 40 per cent of men with hyperthyroidism. Serum concentrations of total estradiol-17beta, total testosterone, and luteinizing hormone are supranormal in these patients. Serum concentration of SHBG is also high in hyperthyroidism. This can explain the high serum testosterone, essentially completely but not the high serum estradiol-17beta; thus, whereas serum unbound testosterone is normal, serum unbound estradiol-17beta is above normal in hyperthyroid men. This balance in relative concentrations of unbound gonadal steroids is apparently quite favorable to the development of gynecomastia in hyperthyroidism. Increased peripheral tissue metabolism of androgens to estrogens seems to be the major factor responsible for high estradiol-17beta in hyperthyroidism; increased glandular secretion of estradiol-17beta may also be important. The mechanism of hypomenorrhea or amenorrhea in hyperthyroidism remains unclear. Changes in circulating estrogens, androgens, and  luteinizing hormone in hyperthyroid women are similar to those in hyperthyroid men. The mid-cycle ovulatory peak of luteinizing hormone may be blunted in patients with scanty periods whereas it may be altogether absent in those with amenorrhea."}
{"id": "PMID:1099360", "title": "Anemia in thyroid diseases.", "content": "Thyroid hormones generally stimulate erythropoiesis. These agents also increase erythrocyte 2,3-DPG concentrations, which serve to enhance the delivery of oxygen to tissues. In the absence of thyroid hormones, anemia frequently develops and may be normocytic, hypochromic-microcytic, or macrocytic. Anemia is an uncommon finding in hyperthyroidism but when present may be morphologically similar to that observed in hypothyroidism. Pernicious anemia has been strongly associated with hypothyroidism, hyperthyroidism, and thyroiditis. Complete correction of anemia often requires restoration of thyroid function as well as specific hematinic therapy. Continued attention to hematologic status is essential in the management of patients with thyroid diseases.", "contents": "Anemia in thyroid diseases. Thyroid hormones generally stimulate erythropoiesis. These agents also increase erythrocyte 2,3-DPG concentrations, which serve to enhance the delivery of oxygen to tissues. In the absence of thyroid hormones, anemia frequently develops and may be normocytic, hypochromic-microcytic, or macrocytic. Anemia is an uncommon finding in hyperthyroidism but when present may be morphologically similar to that observed in hypothyroidism. Pernicious anemia has been strongly associated with hypothyroidism, hyperthyroidism, and thyroiditis. Complete correction of anemia often requires restoration of thyroid function as well as specific hematinic therapy. Continued attention to hematologic status is essential in the management of patients with thyroid diseases."}
{"id": "PMID:1099362", "title": "Thyroiditis: current views of pathogenesis.", "content": "From indirect and circumstantial evidence it would appear that subacute thyroiditis is probably a viral infection of the thyroid gland. The thyroid may respond in the stereotyped manner to any one of a number of viruses. Despite the available evidence, however, the viral theory has yet to be finally proven. Autoimmunity does not appear to be a primary factor in the initiation of subacute thyroiditis, although transient immunologic responses occur as a secondary phenomenon. Autoimmune thyroiditis, on the other hand, is almost certainly a primary immunologic disease, and likely is due to a specific inherited defect in immunologic control. It would appear that both cell-mediated immunity and humoral immunity (i.e., T and B lymphocytes) are necessary for the full expression of this disorder.", "contents": "Thyroiditis: current views of pathogenesis. From indirect and circumstantial evidence it would appear that subacute thyroiditis is probably a viral infection of the thyroid gland. The thyroid may respond in the stereotyped manner to any one of a number of viruses. Despite the available evidence, however, the viral theory has yet to be finally proven. Autoimmunity does not appear to be a primary factor in the initiation of subacute thyroiditis, although transient immunologic responses occur as a secondary phenomenon. Autoimmune thyroiditis, on the other hand, is almost certainly a primary immunologic disease, and likely is due to a specific inherited defect in immunologic control. It would appear that both cell-mediated immunity and humoral immunity (i.e., T and B lymphocytes) are necessary for the full expression of this disorder."}
{"id": "PMID:1099369", "title": "Treatment of carcinoma of the base of the tongue and the larynx.", "content": "Cancer of the base of the tongue and the larynx is usually treated primarily with irradiation to 6,000 rads. Persistence of tumor after irradiation is common and requires a logical, thoughtful approach designed to augment all tissues removed. Careful preoperative planning and the use of the lateral approach to the base of the tongue, in conjunction with pharyngostome and laryngostome formation allows wide resection of the cancer. Preservation of the function of speech and swallowing, and preservation of the mandibular contour are important, and correlate closely with carotid artery protection. Five different methods of approach to the problem are analyzed and presented.", "contents": "Treatment of carcinoma of the base of the tongue and the larynx. Cancer of the base of the tongue and the larynx is usually treated primarily with irradiation to 6,000 rads. Persistence of tumor after irradiation is common and requires a logical, thoughtful approach designed to augment all tissues removed. Careful preoperative planning and the use of the lateral approach to the base of the tongue, in conjunction with pharyngostome and laryngostome formation allows wide resection of the cancer. Preservation of the function of speech and swallowing, and preservation of the mandibular contour are important, and correlate closely with carotid artery protection. Five different methods of approach to the problem are analyzed and presented."}
{"id": "PMID:1099382", "title": "Effects of bacteriophage M13 infection upon phospholipid and fatty acid compositions of Escherichia coli.", "content": "Escherichia coli K38 were grown and infected with wild type and amber mutants of bacteriophage M13 in the early log phase. Lipid compositions of the infected and healthy cultures, grown under identical conditions, were determined 2 hr after infection. From the results, it was observed that total lipid and total phospholipid content remained nearly constant, suggesting that the cell membrane which contained the maximum phospholipids was not damaged by the infection. Moreover, the percentage of diphosphatidylglycerol and lyso- compounds corresponding to phosphatidylethanolamine and phosphatidylglycerol increased, while phosphatidylethanolamine and phosphatidylglycerol decreased. The increase in lyso- compounds may be due to the relase of phospholipase A2 (a periplasmic enzyme) from the cell wall after damage by the infection. Bacteriophage M13 infection had no effect on the fatty acid compostion of the phospholipids.", "contents": "Effects of bacteriophage M13 infection upon phospholipid and fatty acid compositions of Escherichia coli. Escherichia coli K38 were grown and infected with wild type and amber mutants of bacteriophage M13 in the early log phase. Lipid compositions of the infected and healthy cultures, grown under identical conditions, were determined 2 hr after infection. From the results, it was observed that total lipid and total phospholipid content remained nearly constant, suggesting that the cell membrane which contained the maximum phospholipids was not damaged by the infection. Moreover, the percentage of diphosphatidylglycerol and lyso- compounds corresponding to phosphatidylethanolamine and phosphatidylglycerol increased, while phosphatidylethanolamine and phosphatidylglycerol decreased. The increase in lyso- compounds may be due to the relase of phospholipase A2 (a periplasmic enzyme) from the cell wall after damage by the infection. Bacteriophage M13 infection had no effect on the fatty acid compostion of the phospholipids."}
{"id": "PMID:1099399", "title": "Coccidioidomycosis in compromised hosts. Experience at Stanford University Hospital.", "content": "To determine the frequency and clinical characteristics of infection with Coccidioides immitis in immunosuppressed patients at Stanford University Hospital, clinical records of 14 years were examined. Thirteen cases met the diagnostic criteria. Half had Hodgkin's disease. In six the infection was disseminated; five of the six died early in the course of their infectious illness, frequently without diagnosis. Conclusions include: 1. The occurrence of coccidioidomycosis in immunosuppressed patients seen at institutions in or adjacent to the endemic area is not as rare as the literature suggests. 2. Dissemination is frequently explosive and the radiographic appearance of pulmonary involvement may appear late. Widespread pulmonary dissemination may occur within 24 hours after a negative x-ray. 3. Although the skin test loses its diagnostic value, the serology remains valid. Thus immunosuppressed patients with febrile illnesses (with or without radiographically evident pulmonary involvement) who have a history of travel to an endemic area should have serological examinations. 4. Lymphocytopenia correlates with risk of dissemination of coccidioidomycosis. 5. The administration of immunsuppressive chemotherapy correlates with such risk while radiotherapy and the malignant or non-malignant nature of the disease do not.", "contents": "Coccidioidomycosis in compromised hosts. Experience at Stanford University Hospital. To determine the frequency and clinical characteristics of infection with Coccidioides immitis in immunosuppressed patients at Stanford University Hospital, clinical records of 14 years were examined. Thirteen cases met the diagnostic criteria. Half had Hodgkin's disease. In six the infection was disseminated; five of the six died early in the course of their infectious illness, frequently without diagnosis. Conclusions include: 1. The occurrence of coccidioidomycosis in immunosuppressed patients seen at institutions in or adjacent to the endemic area is not as rare as the literature suggests. 2. Dissemination is frequently explosive and the radiographic appearance of pulmonary involvement may appear late. Widespread pulmonary dissemination may occur within 24 hours after a negative x-ray. 3. Although the skin test loses its diagnostic value, the serology remains valid. Thus immunosuppressed patients with febrile illnesses (with or without radiographically evident pulmonary involvement) who have a history of travel to an endemic area should have serological examinations. 4. Lymphocytopenia correlates with risk of dissemination of coccidioidomycosis. 5. The administration of immunsuppressive chemotherapy correlates with such risk while radiotherapy and the malignant or non-malignant nature of the disease do not."}
{"id": "PMID:1099400", "title": "Cytochrome pigments in Mycobacterium leprae isolated from armadillos (Dasypus novemcinctus L.).", "content": "The bacilli were isolated from granulomata harvested from armadillos. Cytochrome systems in whole cell suspensions as well as in cell-free extracts were examined spectrophotometrically. The intact cells contained cytochromes of the a+b3, b and c type which were found to be present mainly in the reduced form. The cytochrome systems in cell-free extracts of M. leprae were in the oxidized form but contained the same type of cytochromes as the intact bacteria. The presence of cytochromes was easily detectable in the anaerobically-reduced (no substrate added) as well as in the dithionite- or succinate-reduced minus O2-oxidized difference spectra. The dithionite-reduced plus CO minus reduced difference spectra exhibited cytochromes a3 and o monoxide binding pigments.", "contents": "Cytochrome pigments in Mycobacterium leprae isolated from armadillos (Dasypus novemcinctus L.). The bacilli were isolated from granulomata harvested from armadillos. Cytochrome systems in whole cell suspensions as well as in cell-free extracts were examined spectrophotometrically. The intact cells contained cytochromes of the a+b3, b and c type which were found to be present mainly in the reduced form. The cytochrome systems in cell-free extracts of M. leprae were in the oxidized form but contained the same type of cytochromes as the intact bacteria. The presence of cytochromes was easily detectable in the anaerobically-reduced (no substrate added) as well as in the dithionite- or succinate-reduced minus O2-oxidized difference spectra. The dithionite-reduced plus CO minus reduced difference spectra exhibited cytochromes a3 and o monoxide binding pigments."}
{"id": "PMID:1099401", "title": "Specific antibodies and heterologous antigensin monofactor sera.", "content": "A concentrated monofactor serum 9 (Wellcome lot K 8028) precipitated against proteins from 2 different strains of Salmonella enteritidis and from a strain of S. dublin, as well as against the proteinic moiety of the somatic antigen from S. typhi 0 901; no polysaccharide from the strains, sharing factor 9, precipitated against this serum. A concentrated monofactor serum 20 (Wellcome lot K 6733) precipitated against the proteins from S. emek (8, 20), but not against its polysaccharide. A monofactor serum 8 (Wellcome lot K 7801), different from a lot previously analysed (Wellcome lot K 6860), precipitated against the same proteins as the previous lot, but, in addition, precipitated strongly against the polysaccharide of S, newport (6.8) and weakly with the polysaccharide of S. emek too. According to these and previous results, it is not clear what sort of antibodies responsible for cross-agglutination are absorbed by the heterologous strains used in the operations. Since more often than not antipolysaccharide antibodies were not found in concentrated monofactor sera, it may be that just the antipolysaccharides. All the monofactor sera so far analysed (Wellcome) 1,2,4,5,8,9,20 are full of proteins left by the bacteria used for absorptions; due to these proteins, the presence of which may be meaningless for the agglutinations, strong precipitations in agar-gel were obtained between monofactor sera and different antibacterial and anti-protein sera containing antibodies induced against the corresponding free proteins.", "contents": "Specific antibodies and heterologous antigensin monofactor sera. A concentrated monofactor serum 9 (Wellcome lot K 8028) precipitated against proteins from 2 different strains of Salmonella enteritidis and from a strain of S. dublin, as well as against the proteinic moiety of the somatic antigen from S. typhi 0 901; no polysaccharide from the strains, sharing factor 9, precipitated against this serum. A concentrated monofactor serum 20 (Wellcome lot K 6733) precipitated against the proteins from S. emek (8, 20), but not against its polysaccharide. A monofactor serum 8 (Wellcome lot K 7801), different from a lot previously analysed (Wellcome lot K 6860), precipitated against the same proteins as the previous lot, but, in addition, precipitated strongly against the polysaccharide of S, newport (6.8) and weakly with the polysaccharide of S. emek too. According to these and previous results, it is not clear what sort of antibodies responsible for cross-agglutination are absorbed by the heterologous strains used in the operations. Since more often than not antipolysaccharide antibodies were not found in concentrated monofactor sera, it may be that just the antipolysaccharides. All the monofactor sera so far analysed (Wellcome) 1,2,4,5,8,9,20 are full of proteins left by the bacteria used for absorptions; due to these proteins, the presence of which may be meaningless for the agglutinations, strong precipitations in agar-gel were obtained between monofactor sera and different antibacterial and anti-protein sera containing antibodies induced against the corresponding free proteins."}
{"id": "PMID:1099402", "title": "Investigation into pyruvate kinases from Escherichia coli K-12 grown under aerobic and anaerobic conditions.", "content": "Two forms of anaerobic Escherichia coli K-12 pyruvate kinase (EC 2.7.1.40) were separated by ammonium sulphate fractionations. Pyruvate kinases I is allosteric and pyruvate kinase II is non-allosteric to phosphoenolpyruvate. The addition of 1 mM FDP reversed the allostery to normal Michaelis-Menten kinetics. AMP had no effect, whereas 8 mM ATP completely inhibited the enzyme. The enzyme showed normal kinetics with ADP as substrate. Mg2+ and Mn2+ stimulated whereas Cu2+ severely inhibited the enzyme, which could be reversed by the addition of 1 mM FDP. Citrate, alpha-ketoglutarate, succinate, fumarate and alanine inhibited the enzyme, whereas phenylalanine had no effect. The allosteric pyruvate kinase from aerobic cultures was not only activated by FDP, but also by AMP. FDP changed Km and Vmax, whereas AMP influenced only the Km. During aerobic-anaerobic transition, pyruvate kinase synthesis increases and reaches a maximum under anaerobic conditions. The degree of FDP activation remains constant, but AMP activation is lost during transition. Aerobic cultures of E. coli K-12 grown on gluconeogenic substrates exhibited pyruvate kinase II activity (non-allosteric), which was stimulated by FDP and by AMP. It has been suggested that E. coli may have two types of pyruvate kinase II depending on the substrate and two types of pyruvate kinase I depending on oxygen tension in the medium.", "contents": "Investigation into pyruvate kinases from Escherichia coli K-12 grown under aerobic and anaerobic conditions. Two forms of anaerobic Escherichia coli K-12 pyruvate kinase (EC 2.7.1.40) were separated by ammonium sulphate fractionations. Pyruvate kinases I is allosteric and pyruvate kinase II is non-allosteric to phosphoenolpyruvate. The addition of 1 mM FDP reversed the allostery to normal Michaelis-Menten kinetics. AMP had no effect, whereas 8 mM ATP completely inhibited the enzyme. The enzyme showed normal kinetics with ADP as substrate. Mg2+ and Mn2+ stimulated whereas Cu2+ severely inhibited the enzyme, which could be reversed by the addition of 1 mM FDP. Citrate, alpha-ketoglutarate, succinate, fumarate and alanine inhibited the enzyme, whereas phenylalanine had no effect. The allosteric pyruvate kinase from aerobic cultures was not only activated by FDP, but also by AMP. FDP changed Km and Vmax, whereas AMP influenced only the Km. During aerobic-anaerobic transition, pyruvate kinase synthesis increases and reaches a maximum under anaerobic conditions. The degree of FDP activation remains constant, but AMP activation is lost during transition. Aerobic cultures of E. coli K-12 grown on gluconeogenic substrates exhibited pyruvate kinase II activity (non-allosteric), which was stimulated by FDP and by AMP. It has been suggested that E. coli may have two types of pyruvate kinase II depending on the substrate and two types of pyruvate kinase I depending on oxygen tension in the medium."}
{"id": "PMID:1099403", "title": "Dilute performic acid--a versatile and easily to handle oxidant in general histology and histochemistry of structure bound sulphur compounds.", "content": "Performic acid normally leads to frequent and nearly inevitable detaching of commonly attached tissue sections, thus rendering such methods unrecommendable for histological and histochemical routine work. By the use of alcohol dilute solutions instead of the crude reagent, this problem can be managed easily. Special attention is drawn to the ambiguity of methods for tissue sulphur, based on the demonstration of oxidation induced strong basophilia.", "contents": "Dilute performic acid--a versatile and easily to handle oxidant in general histology and histochemistry of structure bound sulphur compounds. Performic acid normally leads to frequent and nearly inevitable detaching of commonly attached tissue sections, thus rendering such methods unrecommendable for histological and histochemical routine work. By the use of alcohol dilute solutions instead of the crude reagent, this problem can be managed easily. Special attention is drawn to the ambiguity of methods for tissue sulphur, based on the demonstration of oxidation induced strong basophilia."}
{"id": "PMID:1099405", "title": "[Intravital accumulation of acriflavine in mitochondria of yeast organisms].", "content": "Acriflavine, a fluorochrome of the acridine series, inducing cytoplasmic mutations in yeast organisms, at low concentrations (0.25 to 2.50 mcg/ml) in the growth medium, is selectively accumulated in the mitochondria in which it forms complexes with nucleic acids. This binding of acriflavine to nucleic acids in the mitochondria has almost no effect on the cell viability though their respiratory activity decreases at these concentrations by 70 percent, and the activity of fermentation increases by 15 to 20 percent.", "contents": "[Intravital accumulation of acriflavine in mitochondria of yeast organisms]. Acriflavine, a fluorochrome of the acridine series, inducing cytoplasmic mutations in yeast organisms, at low concentrations (0.25 to 2.50 mcg/ml) in the growth medium, is selectively accumulated in the mitochondria in which it forms complexes with nucleic acids. This binding of acriflavine to nucleic acids in the mitochondria has almost no effect on the cell viability though their respiratory activity decreases at these concentrations by 70 percent, and the activity of fermentation increases by 15 to 20 percent."}
{"id": "PMID:1099406", "title": "[New variant of the method for direct count of bacteria in water].", "content": "Direct counting of the bacteria in water is suggested to be done by means of the pitch limit stop of the microscope tube made as a washer with stop pins.", "contents": "[New variant of the method for direct count of bacteria in water]. Direct counting of the bacteria in water is suggested to be done by means of the pitch limit stop of the microscope tube made as a washer with stop pins."}
{"id": "PMID:1099408", "title": "[Production and biological properties of L-like mutants (11r) of Escherichia coli].", "content": "Treatment of the strains of E. coli K12 with the mutagen N-nitroso-N-methylurea yielded mutants with the morphology similar to that of stable penicillin L-forms. Studies of the biological properties of the cultures have shown that the mutation of the gen 11r increases the susceptibility to antibiotics of various action spectra, ionic and non-ionic detergents and the resistance to UV and gamma radiation. Biochemical analysis of peptidoglycan from the cell walls of the parent bacterial cultures and L-mutants revealed no differences in its chemical composition.", "contents": "[Production and biological properties of L-like mutants (11r) of Escherichia coli]. Treatment of the strains of E. coli K12 with the mutagen N-nitroso-N-methylurea yielded mutants with the morphology similar to that of stable penicillin L-forms. Studies of the biological properties of the cultures have shown that the mutation of the gen 11r increases the susceptibility to antibiotics of various action spectra, ionic and non-ionic detergents and the resistance to UV and gamma radiation. Biochemical analysis of peptidoglycan from the cell walls of the parent bacterial cultures and L-mutants revealed no differences in its chemical composition."}
{"id": "PMID:1099409", "title": "[Ecology of the obligate thermophilic bacteria].", "content": "The obligate thermophilic non-sporeforming bacterium Thermus ruber, isolated from the thermal springs of Kamchatka at temperatures of 60 to 90 degrees C (Loginova et al.,1974), has a high lytic activity towards the living and dead cells of Escherichia coli and Micrococcus lysodeikticus.", "contents": "[Ecology of the obligate thermophilic bacteria]. The obligate thermophilic non-sporeforming bacterium Thermus ruber, isolated from the thermal springs of Kamchatka at temperatures of 60 to 90 degrees C (Loginova et al.,1974), has a high lytic activity towards the living and dead cells of Escherichia coli and Micrococcus lysodeikticus."}
{"id": "PMID:1099407", "title": "[Effect of heliomycin on protein and nucleic acid synthesis in Escherichia coli].", "content": "Heliomycin at a concentration of 100 mcg/ml had almost no effect on the growth of Escherichia coli, which was inhibited only upon the combined action of heliomycin and ethanol that facilitated the penetration of the antibiotic into the cell. Heliomycin inhibited in these conditions the synthesis of RNA and had no effect on the synthesis of DNA in the three studied strains of E. coli. The synthesis of protein either was not inhibited by heliomycin at all or was inhibited only to a small extent.", "contents": "[Effect of heliomycin on protein and nucleic acid synthesis in Escherichia coli]. Heliomycin at a concentration of 100 mcg/ml had almost no effect on the growth of Escherichia coli, which was inhibited only upon the combined action of heliomycin and ethanol that facilitated the penetration of the antibiotic into the cell. Heliomycin inhibited in these conditions the synthesis of RNA and had no effect on the synthesis of DNA in the three studied strains of E. coli. The synthesis of protein either was not inhibited by heliomycin at all or was inhibited only to a small extent."}
{"id": "PMID:1099437", "title": "Evidence for a functional association of DNA synthesis with the membrane in mitochondria of Saccharomyces cerevisiae.", "content": "We have studied the effect of membrane fatty acid composition on replicative DNA synthetic activity in mitochondria isolated from Saccharomyces cerevisiae. Cells containing different levels of membrane unsaturated fatty acids were obtained by growth of a fatty acid desaturase mutant of Saccharomyces cerevisiae in glucose-limited chemostat cultures supplemented with various concentrations of Tween 80. Arrhenius plots of DNA synthetic activity in isolated mitochondria show a discrete discontinuity at specific temperature which are dependent on the membrane unsaturated fatty acid content of the mitochondria. This indicates a functional association of DNA replication with the mitochondrial membrane in Saccharomyces cerevisiae.", "contents": "Evidence for a functional association of DNA synthesis with the membrane in mitochondria of Saccharomyces cerevisiae. We have studied the effect of membrane fatty acid composition on replicative DNA synthetic activity in mitochondria isolated from Saccharomyces cerevisiae. Cells containing different levels of membrane unsaturated fatty acids were obtained by growth of a fatty acid desaturase mutant of Saccharomyces cerevisiae in glucose-limited chemostat cultures supplemented with various concentrations of Tween 80. Arrhenius plots of DNA synthetic activity in isolated mitochondria show a discrete discontinuity at specific temperature which are dependent on the membrane unsaturated fatty acid content of the mitochondria. This indicates a functional association of DNA replication with the mitochondrial membrane in Saccharomyces cerevisiae."}
{"id": "PMID:1099438", "title": "Complementary binding of oligonucleotides with 16S RNA and ribosomal ribonucleoproteins.", "content": "The accessibility of single-stranded sequences in 16S RNA in free state and in ribonucleoprotein particles (RNP) to complementary binding with isoplith fractions of oligonucleotides was studied. RNP had different protein composition and corresponded to intermediate stages of E. coli 30S subunit assembly in vitro. Gel-filtration was used to detect the most strong binding. It was found that S4 essentially inhibited the hexamer binding to RNA. 'Core' proteins bound to 16S RNA strongly increased the shielding of single-stranded regions while 'split' proteins insignificantly changed the hexamer binding. Nevertheless evidence is presented that 'split' proteins might also interact directly with 16S RNA in the 30S subunit.", "contents": "Complementary binding of oligonucleotides with 16S RNA and ribosomal ribonucleoproteins. The accessibility of single-stranded sequences in 16S RNA in free state and in ribonucleoprotein particles (RNP) to complementary binding with isoplith fractions of oligonucleotides was studied. RNP had different protein composition and corresponded to intermediate stages of E. coli 30S subunit assembly in vitro. Gel-filtration was used to detect the most strong binding. It was found that S4 essentially inhibited the hexamer binding to RNA. 'Core' proteins bound to 16S RNA strongly increased the shielding of single-stranded regions while 'split' proteins insignificantly changed the hexamer binding. Nevertheless evidence is presented that 'split' proteins might also interact directly with 16S RNA in the 30S subunit."}
{"id": "PMID:1099445", "title": "Efficacy of 17alpha-hydroxyprogesterone caproate in the prevention of premature labor.", "content": "We conducted a double-blind study to determine the efficacy of 17alpha-hydroxyprogesterone caproate in preventing premature delivery in 43 high-risk patients. Premature delivery did not occur in 18 patients receiving the progestational agent, whereas 41 per cent of the 22 receiving the palcebo had premature delivery (P less than 0.01). The mean duration of pregnancy and the mean birth weight in the former group (38.6 weeks +/- 1.6 S.D., and 2836 g +/- 412 S.D.) were both significantly greater (P less than 0.025) than that in the latter (35.2 weeks +/- 6.7 S.D.; 2361 g +/- 1085 S.D.). The perinatal mortality rate in the group given the progestational agent (O per cent) was significantly less than that observed in the placebo group (27 per cent) (P less than 0.05). Although there were no complications attributable to the progestational drug, the study population was too small for assessment of immediate or long term safety. However, the results indicate a possible obstetric use for this drug.", "contents": "Efficacy of 17alpha-hydroxyprogesterone caproate in the prevention of premature labor. We conducted a double-blind study to determine the efficacy of 17alpha-hydroxyprogesterone caproate in preventing premature delivery in 43 high-risk patients. Premature delivery did not occur in 18 patients receiving the progestational agent, whereas 41 per cent of the 22 receiving the palcebo had premature delivery (P less than 0.01). The mean duration of pregnancy and the mean birth weight in the former group (38.6 weeks +/- 1.6 S.D., and 2836 g +/- 412 S.D.) were both significantly greater (P less than 0.025) than that in the latter (35.2 weeks +/- 6.7 S.D.; 2361 g +/- 1085 S.D.). The perinatal mortality rate in the group given the progestational agent (O per cent) was significantly less than that observed in the placebo group (27 per cent) (P less than 0.05). Although there were no complications attributable to the progestational drug, the study population was too small for assessment of immediate or long term safety. However, the results indicate a possible obstetric use for this drug."}
{"id": "PMID:1099449", "title": "Antiemetic effect of delta-9-tetrahydrocannabinol in patients receiving cancer chemotherapy.", "content": "Anecdotal accounts suggested that smoking marihuana decreases the nausea and vomiting associated with cancer chemotherapeutic agents. Oral delta-9-tetrahydrocannabinol was compared with placebo in a controlled, randomized, \"double-blind\" experiment. All patients were receiving chemotherapeutic drugs known to cause nausea and vomiting of central origin. Each patient was to serve as his own control to determine whether tetrahydrocannabinol had an antiemetic effect. Twenty-two patients entered the study, 20 of whom were evaluable. For all patients an antiemetic effect was observed in 14 of 20 tetrahydrocannabinol courses and in none of 22 placebo courses. For patients completing the study, response occurred in 12 of 15 courses of tetrahydrocannabinol and in none of 14 courses of placebo (P less than 0.001). No patient vomited while experiencing a subjective \"high\". Oral tetrahydrocannabinol has antiemetic properties and is significantly better than a placebo in reducting vomiting caused by chemotherapeutic agents.", "contents": "Antiemetic effect of delta-9-tetrahydrocannabinol in patients receiving cancer chemotherapy. Anecdotal accounts suggested that smoking marihuana decreases the nausea and vomiting associated with cancer chemotherapeutic agents. Oral delta-9-tetrahydrocannabinol was compared with placebo in a controlled, randomized, \"double-blind\" experiment. All patients were receiving chemotherapeutic drugs known to cause nausea and vomiting of central origin. Each patient was to serve as his own control to determine whether tetrahydrocannabinol had an antiemetic effect. Twenty-two patients entered the study, 20 of whom were evaluable. For all patients an antiemetic effect was observed in 14 of 20 tetrahydrocannabinol courses and in none of 22 placebo courses. For patients completing the study, response occurred in 12 of 15 courses of tetrahydrocannabinol and in none of 14 courses of placebo (P less than 0.001). No patient vomited while experiencing a subjective \"high\". Oral tetrahydrocannabinol has antiemetic properties and is significantly better than a placebo in reducting vomiting caused by chemotherapeutic agents."}
{"id": "PMID:1099453", "title": "Genetic perturbations that reveal tertiary conformation of tRNA precursor molecules.", "content": "The ubiquity of tRNA-like conformations in tRNA precursors of prokaryotes provides a common structural basis for precursor recognition by the maturation enzymes. This design eliminates need for multiple enzymes to achieve the maturation of different precursors. Moreover, the requirement of this specific conformation for maturation guards against the production of potentially deleterious mutant tRNAs.", "contents": "Genetic perturbations that reveal tertiary conformation of tRNA precursor molecules. The ubiquity of tRNA-like conformations in tRNA precursors of prokaryotes provides a common structural basis for precursor recognition by the maturation enzymes. This design eliminates need for multiple enzymes to achieve the maturation of different precursors. Moreover, the requirement of this specific conformation for maturation guards against the production of potentially deleterious mutant tRNAs."}
{"id": "PMID:1099458", "title": "In vitro synthesis of tRNA precursors and their conversion to mature size tRNA.", "content": "Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+ and su3-) and tRNA2Tyr, tRNA2Gly (su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.", "contents": "In vitro synthesis of tRNA precursors and their conversion to mature size tRNA. Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+ and su3-) and tRNA2Tyr, tRNA2Gly (su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules."}
{"id": "PMID:1099463", "title": "The importance of sensory nerve endings as sites of drug action.", "content": "The role that sensory nerve endings can play in drug action and the strategy used for its experimental analysis and proof is first exemplified by three effects of nicotine which are seen when the lowest effective doses of the drug are given intravenously in the cat: (1) a vasopressor effect due to arterial chemoreceptor stimulation; (2) a triad of bradycardia, hypotension and apnea, and (3) a depressant effect upon somatic motor activity, both of which are traced to vagal afferent endings in the pulmonary circulation. While receptors in the lung are responsible at least for the initial phase of the reflex responses listed in (2) and (3), sensory endings in heart, aorta, and carotid sinus region may be recruited into action as the drug reaches them. Several of these reflex effects can also be elicited by other sensory stimulant agents such as phenyldiguanide, 5-hydroxytryptamine, and veratrum alkaloids. In the second part, a general outline is given of what may be classified as 'Afferent Pharmacology', dealing with drug action upon sensory receptors and with the resulting remote drug effects. The action upon sensory receptors can either be a direct one ('primary' drug effect) consisting of stimulation, sensitization, desensitization, depression or combinations thereof, or an indirect ('secondary') effect brought about by a variety of drug-induced changes in the tissues surrounding the receptors. Depending on the nature of the primary or secondary action, the remote drug effect can be either an initiation, modification or impairment of those reflexes which have their origin in the sensory endings acted upon. Indeed, the grossly observable pharmacological actions of 'afferent drugs' are generally those relating to the reflex response. To avoid blurring of the boundaries of afferent pharmacology, drugs acting on central synapses of reflex pathways, or on the elaborate efferent control system of afferent input, are not included. A discussion follows of the topics of investigation, the influence of experimental conditions and anesthesia, various approaches and methods, the physiological and pharmacological importance of inquiry in this area, and some of the therapeutic aspects. Finally, brief mention is made of certain features and problems which appear to be characteristic of afferent pharmacology.", "contents": "The importance of sensory nerve endings as sites of drug action. The role that sensory nerve endings can play in drug action and the strategy used for its experimental analysis and proof is first exemplified by three effects of nicotine which are seen when the lowest effective doses of the drug are given intravenously in the cat: (1) a vasopressor effect due to arterial chemoreceptor stimulation; (2) a triad of bradycardia, hypotension and apnea, and (3) a depressant effect upon somatic motor activity, both of which are traced to vagal afferent endings in the pulmonary circulation. While receptors in the lung are responsible at least for the initial phase of the reflex responses listed in (2) and (3), sensory endings in heart, aorta, and carotid sinus region may be recruited into action as the drug reaches them. Several of these reflex effects can also be elicited by other sensory stimulant agents such as phenyldiguanide, 5-hydroxytryptamine, and veratrum alkaloids. In the second part, a general outline is given of what may be classified as 'Afferent Pharmacology', dealing with drug action upon sensory receptors and with the resulting remote drug effects. The action upon sensory receptors can either be a direct one ('primary' drug effect) consisting of stimulation, sensitization, desensitization, depression or combinations thereof, or an indirect ('secondary') effect brought about by a variety of drug-induced changes in the tissues surrounding the receptors. Depending on the nature of the primary or secondary action, the remote drug effect can be either an initiation, modification or impairment of those reflexes which have their origin in the sensory endings acted upon. Indeed, the grossly observable pharmacological actions of 'afferent drugs' are generally those relating to the reflex response. To avoid blurring of the boundaries of afferent pharmacology, drugs acting on central synapses of reflex pathways, or on the elaborate efferent control system of afferent input, are not included. A discussion follows of the topics of investigation, the influence of experimental conditions and anesthesia, various approaches and methods, the physiological and pharmacological importance of inquiry in this area, and some of the therapeutic aspects. Finally, brief mention is made of certain features and problems which appear to be characteristic of afferent pharmacology."}
{"id": "PMID:1099465", "title": "Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli.", "content": "There is a difference in the extent of inhibition of thymine dimers (TT) excision in ultravioley (UV) irradiated cells of E. coli after preirradiation depression of protein and DNA syntheses induced by a simultaneous deprivation of essential amino acids (AA-) and thymine(T-) or by deprivation of essential amino acids and addition of nalidixie acid (NAL+). This difference has been noted in both E. coli B/r Her+ and E. coli K12 SR20 uvr+ cells. Depression of DNA synthesis with the aid of malidixic acid as exogenous agent will inhibit TT excision to a lesser degree than depression of DNA synthesis by thymine starvation. The extent of TT excision has no appreciable influence on restoration of the sedimentation profile of newly synthesized DNA nor again on UV resistance of cells in conditions of dark repair. At the time when there are TT still present in DNA, the DNA molecule, having the size of the molecule of unirradiated cells, will become synthesized.", "contents": "Comparison of the effect of nalidixic acid and thymine deprivation on excision repair in Escherichia coli. There is a difference in the extent of inhibition of thymine dimers (TT) excision in ultravioley (UV) irradiated cells of E. coli after preirradiation depression of protein and DNA syntheses induced by a simultaneous deprivation of essential amino acids (AA-) and thymine(T-) or by deprivation of essential amino acids and addition of nalidixie acid (NAL+). This difference has been noted in both E. coli B/r Her+ and E. coli K12 SR20 uvr+ cells. Depression of DNA synthesis with the aid of malidixic acid as exogenous agent will inhibit TT excision to a lesser degree than depression of DNA synthesis by thymine starvation. The extent of TT excision has no appreciable influence on restoration of the sedimentation profile of newly synthesized DNA nor again on UV resistance of cells in conditions of dark repair. At the time when there are TT still present in DNA, the DNA molecule, having the size of the molecule of unirradiated cells, will become synthesized."}
{"id": "PMID:1099466", "title": "Changes in stored and short-term cultures of mouse bone marrow cells.", "content": "Changes in cells of mouse bone marrow cultured for 6--24 hr were followed both when fresh, and after storage for 3 to 5 days at a temperature of +2 to +4 degrees C. Considerable alterations were observed in the morphology, activity and transformation of these cells both during the storage period and the subsequent culture. Phagocyte ability was also detected in these cultures.", "contents": "Changes in stored and short-term cultures of mouse bone marrow cells. Changes in cells of mouse bone marrow cultured for 6--24 hr were followed both when fresh, and after storage for 3 to 5 days at a temperature of +2 to +4 degrees C. Considerable alterations were observed in the morphology, activity and transformation of these cells both during the storage period and the subsequent culture. Phagocyte ability was also detected in these cultures."}
{"id": "PMID:1099468", "title": "Therapeutic effect of a water-salt extract from BCG in two patients with advanced breast cancer.", "content": "Two cases of advanced breast cancer treated with a water-salt extract from BCG are presented, showing a positive response -- marked and durable regression of the breast tumor and of the axillar metastases, parallel in the second case with a disappearance of lung metastases.", "contents": "Therapeutic effect of a water-salt extract from BCG in two patients with advanced breast cancer. Two cases of advanced breast cancer treated with a water-salt extract from BCG are presented, showing a positive response -- marked and durable regression of the breast tumor and of the axillar metastases, parallel in the second case with a disappearance of lung metastases."}
{"id": "PMID:1099469", "title": "Nonspecific resistance to tumor allograft.", "content": "The effects of an increased volume of reticulohistiocyte system and macrophage activation in the recipient after treatment with BCG and Zymosan on the course of antitumor concomitant allograft-immunity reaction were studied. The results were verified on animals affected with immunosuppression due to whole body irradiation. A factor of essential significance for resistance to tumor allograft is an increase of the total number of small lymphocytes supplied by the transplantation of syngeneic spleen cells. A substantially lower efficiency was shown by prophylactic administration of BCG and Zymosan. Transplantation of macrophages syngeneic to the host failed to affect the latter's resistance to subsequent tumor allograft. The irradiated host's reactivity was regenerated with a suspesion of spleen cells of purified small lymphocytes, but transplantation of syngeneic macrophages remained without effect.", "contents": "Nonspecific resistance to tumor allograft. The effects of an increased volume of reticulohistiocyte system and macrophage activation in the recipient after treatment with BCG and Zymosan on the course of antitumor concomitant allograft-immunity reaction were studied. The results were verified on animals affected with immunosuppression due to whole body irradiation. A factor of essential significance for resistance to tumor allograft is an increase of the total number of small lymphocytes supplied by the transplantation of syngeneic spleen cells. A substantially lower efficiency was shown by prophylactic administration of BCG and Zymosan. Transplantation of macrophages syngeneic to the host failed to affect the latter's resistance to subsequent tumor allograft. The irradiated host's reactivity was regenerated with a suspesion of spleen cells of purified small lymphocytes, but transplantation of syngeneic macrophages remained without effect."}
{"id": "PMID:1099470", "title": "Lowering of the tumoricide activity of human serum with Varidase. II. Antibodies against Varidase in human serum measured by complement binding.", "content": "Active human serum manifests a tumoricide effect against cells of the Ehrlich ascites tumor. Sera of patients with Ca ventriculi show a higher effect than those of patients with a chronic streptococcal disorder, in comparison with intact subjects. This tumorcide effect is depressed by Varidase, the degree of depression being greatest in the group of patients with streptococcal infection, smaller in healthy subjects and least in carcinoma patients. The investigated sera contained antibodies against Varidase, but not against streptokinase. The anti-Varidase antibodies are found also in sera of the new-born children. The decline of the tumoricide activity of Varidase-treated sera is related to the quantity of anti-Varidase antibodies determined by complement fixing reaction in non-treated sera.", "contents": "Lowering of the tumoricide activity of human serum with Varidase. II. Antibodies against Varidase in human serum measured by complement binding. Active human serum manifests a tumoricide effect against cells of the Ehrlich ascites tumor. Sera of patients with Ca ventriculi show a higher effect than those of patients with a chronic streptococcal disorder, in comparison with intact subjects. This tumorcide effect is depressed by Varidase, the degree of depression being greatest in the group of patients with streptococcal infection, smaller in healthy subjects and least in carcinoma patients. The investigated sera contained antibodies against Varidase, but not against streptokinase. The anti-Varidase antibodies are found also in sera of the new-born children. The decline of the tumoricide activity of Varidase-treated sera is related to the quantity of anti-Varidase antibodies determined by complement fixing reaction in non-treated sera."}
{"id": "PMID:1099471", "title": "Evaluation of the usefulness of nitroblue tetrazolium reduction test (NBT test) for detection of bacterial infection in cancer patients.", "content": "False positivity of the NBT test in neoplastic diseases described by some authose may be a limitation in the use of the test for infection screening in cancer patients. For explaining this problem studies were performed in a group of 30 patients with various untreated malignancies without infection and in 20 of these with bacteriologically confirmed infections. The obtained results were compared with those in groups of normal persons without and with infection. Slightly modified Park method for spontaneous and stimulated NBT test was used. No significant differences between patients of both groups without infection were found. The increase in positivity of the test in presence of infection was lower in cancer patients than in normal subjects, however, it was non-significant. We did not observe any false-positive results in our patients, though one case of false negativity in myeloma is described separately. It is concluded that neoplastic disease does not cause, as such positivity of the NBT test. Because of similar response of NBT test to presence of infection, the NBT test is recognized as very useful for infection screening in cancer patients similarly as in normal persons.", "contents": "Evaluation of the usefulness of nitroblue tetrazolium reduction test (NBT test) for detection of bacterial infection in cancer patients. False positivity of the NBT test in neoplastic diseases described by some authose may be a limitation in the use of the test for infection screening in cancer patients. For explaining this problem studies were performed in a group of 30 patients with various untreated malignancies without infection and in 20 of these with bacteriologically confirmed infections. The obtained results were compared with those in groups of normal persons without and with infection. Slightly modified Park method for spontaneous and stimulated NBT test was used. No significant differences between patients of both groups without infection were found. The increase in positivity of the test in presence of infection was lower in cancer patients than in normal subjects, however, it was non-significant. We did not observe any false-positive results in our patients, though one case of false negativity in myeloma is described separately. It is concluded that neoplastic disease does not cause, as such positivity of the NBT test. Because of similar response of NBT test to presence of infection, the NBT test is recognized as very useful for infection screening in cancer patients similarly as in normal persons."}
{"id": "PMID:1099473", "title": "Cellular hypersensitization to peripheral nervous antigens in the Guillain-Barr\u00e9 syndrome.", "content": "The macrophage migration inhibition factor assay was used as a specific measure of cellular hypersensitivity to peripheral nervous system antigen in a large group of Guillain-Barre patients and control subjects. Lymphocytes from 34 patients with Guillain-Barre syndrome, 33 with other peripheral nervous system disease, and 33 normal controls were assayed for production of macrophage migration inhibition factor. A mean of 101 +/- 7.2 was obtained in the control group, 70 +/- 16.3 in the Guillain-Barre syndrome group, and 96 +/- 11.3 in those with other peripheral nervous system disease. Twenty-six of the 34 patients with Guillain-Barre syndrome, two patients with myeloradiculitis, and two with Bell's palsy gave significant values. These results support the hypothesis that cellular hypersensitization to peripheral nervous system antigens is a pathogenetic factor in Guillain-Barre syndrome.", "contents": "Cellular hypersensitization to peripheral nervous antigens in the Guillain-Barr\u00e9 syndrome. The macrophage migration inhibition factor assay was used as a specific measure of cellular hypersensitivity to peripheral nervous system antigen in a large group of Guillain-Barre patients and control subjects. Lymphocytes from 34 patients with Guillain-Barre syndrome, 33 with other peripheral nervous system disease, and 33 normal controls were assayed for production of macrophage migration inhibition factor. A mean of 101 +/- 7.2 was obtained in the control group, 70 +/- 16.3 in the Guillain-Barre syndrome group, and 96 +/- 11.3 in those with other peripheral nervous system disease. Twenty-six of the 34 patients with Guillain-Barre syndrome, two patients with myeloradiculitis, and two with Bell's palsy gave significant values. These results support the hypothesis that cellular hypersensitization to peripheral nervous system antigens is a pathogenetic factor in Guillain-Barre syndrome."}
{"id": "PMID:1099475", "title": "Emergency medical services in crisis: an Italian case study.", "content": "This paper deals with the system of emergency medical services in Italy. More specifically, it is a case study of the organization and operation of this system in the region of Tuscany. Recent decentralization decrees have established regional governments with major responsibilities for health care, including emergency medical services. The effects of a long history of social and political cleavages on provision of these services at the regional level are presented and discussed. The paper concludes that prospects for rational reform of emergency care service are dim.", "contents": "Emergency medical services in crisis: an Italian case study. This paper deals with the system of emergency medical services in Italy. More specifically, it is a case study of the organization and operation of this system in the region of Tuscany. Recent decentralization decrees have established regional governments with major responsibilities for health care, including emergency medical services. The effects of a long history of social and political cleavages on provision of these services at the regional level are presented and discussed. The paper concludes that prospects for rational reform of emergency care service are dim."}
{"id": "PMID:1099479", "title": "[R. D. Laing's evaluation and critical methodology in the problems of psychological sciences].", "content": "The theory and methodology of psychotherapy and contemporary psychiatrics according to R. D. Laing are presented and examined from a critical point of view.", "contents": "[R. D. Laing's evaluation and critical methodology in the problems of psychological sciences]. The theory and methodology of psychotherapy and contemporary psychiatrics according to R. D. Laing are presented and examined from a critical point of view."}
{"id": "PMID:1099489", "title": "Ketoprofen (Orudis) in ankylosing spondylitis.", "content": "A double-blind cross-over study is described in which ketoprofen (Orudis) is compared with phenylbutazone in 14 patients with ankylosing spondylitis. Results indicated a significant patient preference for phenylbutazone with greater improvement in the ESR. However, the absence of objective and laboratory deterioration with ketoprofen, when compared with the initial, or control, assessments, suggests that it may prove a useful alternative to phenylbutazone in spondylitic disease.", "contents": "Ketoprofen (Orudis) in ankylosing spondylitis. A double-blind cross-over study is described in which ketoprofen (Orudis) is compared with phenylbutazone in 14 patients with ankylosing spondylitis. Results indicated a significant patient preference for phenylbutazone with greater improvement in the ESR. However, the absence of objective and laboratory deterioration with ketoprofen, when compared with the initial, or control, assessments, suggests that it may prove a useful alternative to phenylbutazone in spondylitic disease."}
{"id": "PMID:1099490", "title": "Urinary antiseptics in asymptomatic bacteriuria of pregnancy.", "content": "A screening programme for the detection of asymptomatic bacteriuria in patients attending an antenatal clinic is described. The results obtained from the use of urinary antiseptics in a controlled trial over a period of two years are presented. The number of patients developing clinical pyelonephritis, fetal maturity at delivery and birth weight were recorded for treatment and control groups and compared with the equivalent results obtained from the total population of patients seen at the clinic.", "contents": "Urinary antiseptics in asymptomatic bacteriuria of pregnancy. A screening programme for the detection of asymptomatic bacteriuria in patients attending an antenatal clinic is described. The results obtained from the use of urinary antiseptics in a controlled trial over a period of two years are presented. The number of patients developing clinical pyelonephritis, fetal maturity at delivery and birth weight were recorded for treatment and control groups and compared with the equivalent results obtained from the total population of patients seen at the clinic."}
{"id": "PMID:1099491", "title": "A double blind controlled trial of a new anorectic agent AN448.", "content": "AN448 is a new anorectic agent, which in the dose used, is without the CNS stimulant properties of the amphetamines. Patients entering the trial were a highly resistant group at least 20 percent above ideal weight chosen from an ischaemic-hyperlipidaemic clinic population. In a double blind trial of 12 patients on the active drug and nine patients on placebo, a highly significant weight loss occurred over 12 weeks in the active group only. A significant reduction in serum cholesterol occurred independently of weight loss but there was no reduction in the serum triglycerides and no change in the lipoprotein electrophoretic pattern. The drug is shown to be effective for this group of patients and may also be useful as a cholesterol lowering agent.", "contents": "A double blind controlled trial of a new anorectic agent AN448. AN448 is a new anorectic agent, which in the dose used, is without the CNS stimulant properties of the amphetamines. Patients entering the trial were a highly resistant group at least 20 percent above ideal weight chosen from an ischaemic-hyperlipidaemic clinic population. In a double blind trial of 12 patients on the active drug and nine patients on placebo, a highly significant weight loss occurred over 12 weeks in the active group only. A significant reduction in serum cholesterol occurred independently of weight loss but there was no reduction in the serum triglycerides and no change in the lipoprotein electrophoretic pattern. The drug is shown to be effective for this group of patients and may also be useful as a cholesterol lowering agent."}
{"id": "PMID:1099494", "title": "The father of radiology in New Zealand.", "content": "Attention is drawn to the pioneering contribution of Dr Henry A. de Lautour to the practice of radiology in New Zealand and a brief sketch of his life and work is recorded. Information has been gathered from one of his patients who still possesses a copy of a radiograpy taken by Dr de Lautour in 1889, just four years after the discovery of x-rays. A 75-year follow-up radiograph shows that all but one of the lead pellets still remain in his hand.", "contents": "The father of radiology in New Zealand. Attention is drawn to the pioneering contribution of Dr Henry A. de Lautour to the practice of radiology in New Zealand and a brief sketch of his life and work is recorded. Information has been gathered from one of his patients who still possesses a copy of a radiograpy taken by Dr de Lautour in 1889, just four years after the discovery of x-rays. A 75-year follow-up radiograph shows that all but one of the lead pellets still remain in his hand."}
{"id": "PMID:1099496", "title": "Clinical experience with the oxytocin challenge test. II. An ominous atypical pattern.", "content": "Three cases have been observed over the past 3 years at Los Angeles County-USC Medical Center, Women's Hospital, which have shown an unusual fetal heart rate response to induced uterine contractions during the antepartum period. All 3 cases resulted in perinatal death apparently due to asphyxia. This report describes this unusual pattern and presents a discussion of its possible significance.", "contents": "Clinical experience with the oxytocin challenge test. II. An ominous atypical pattern. Three cases have been observed over the past 3 years at Los Angeles County-USC Medical Center, Women's Hospital, which have shown an unusual fetal heart rate response to induced uterine contractions during the antepartum period. All 3 cases resulted in perinatal death apparently due to asphyxia. This report describes this unusual pattern and presents a discussion of its possible significance."}
{"id": "PMID:1099497", "title": "Intraepithelial vaginal neoplasia following immunosuppressive therapy treated with topical 5-Fu.", "content": "A case is reported of the successful treatment of multifocal intraepithelial neoplasia of the vagina by topical application of a cytotoxic agent, 5-fluorouracil. These lesions followed prolonged immunosuppressive therapy after renal transplantation. Intraepithelial neoplasia was present also in the cervix and was treated by conization.", "contents": "Intraepithelial vaginal neoplasia following immunosuppressive therapy treated with topical 5-Fu. A case is reported of the successful treatment of multifocal intraepithelial neoplasia of the vagina by topical application of a cytotoxic agent, 5-fluorouracil. These lesions followed prolonged immunosuppressive therapy after renal transplantation. Intraepithelial neoplasia was present also in the cervix and was treated by conization."}
{"id": "PMID:1099501", "title": "Combination chemotherapy in disseminated melanoma and other solid tumors in adults.", "content": "Eighty-eight patients with disseminated melanoma and 22 patients with various other solid tumors were treated with combination chemotherapy consisting of DTIC, at a fixed dose, with either adriamycin, CCNU, or hydroxyurea, at several dosages.  An objective response rate of 20% was observed in 84 evaluable melanoma patients.  The addition of the other agents to DTIC did not provide enhanced antitumor activity.  Combination chemotherapy increased toxicity.  Encouraging objective responses were observed in patients with synovial sarcoma (4/7) and teratocarcinoma of the testis (1/1) treated with the combination of adriamycin and DTIC.", "contents": "Combination chemotherapy in disseminated melanoma and other solid tumors in adults. Eighty-eight patients with disseminated melanoma and 22 patients with various other solid tumors were treated with combination chemotherapy consisting of DTIC, at a fixed dose, with either adriamycin, CCNU, or hydroxyurea, at several dosages.  An objective response rate of 20% was observed in 84 evaluable melanoma patients.  The addition of the other agents to DTIC did not provide enhanced antitumor activity.  Combination chemotherapy increased toxicity.  Encouraging objective responses were observed in patients with synovial sarcoma (4/7) and teratocarcinoma of the testis (1/1) treated with the combination of adriamycin and DTIC."}
{"id": "PMID:1099507", "title": "Surgical treatment of the swan-neck deformity in rheumatoid arthritis.", "content": "Swan-neck deformities vary not only in origin but also in the extent of functional loss. Careful examination used to determine the degree of flexion in all positions as well as x-ray examination of the joint has allowed us to group these patients into four types. Various surgical procedures are recommended according to this classification, which has made the treatment of these patients more orderly and rewarding.", "contents": "Surgical treatment of the swan-neck deformity in rheumatoid arthritis. Swan-neck deformities vary not only in origin but also in the extent of functional loss. Careful examination used to determine the degree of flexion in all positions as well as x-ray examination of the joint has allowed us to group these patients into four types. Various surgical procedures are recommended according to this classification, which has made the treatment of these patients more orderly and rewarding."}
{"id": "PMID:1099508", "title": "Surgical treatment of the boutonniere deformity in rheumatoid arthritis.", "content": "The rheumatoid boutonniere deformity does not limit hand function significantly until it becomes severe. For this reason the surgical procedures used should not risk or sacrifice existing function. For the mild cases one can improve the digital balance by a simple extensor tenotomy combined with dynamic splinting. In more advanced stages with passive correction possible, the deformity can be improved by reconstruction of the extensor mechanism. With fixed deformities one can choose between proximal interphalangeal joint fusion and arthroplasty. The decision is influenced by several factors, including the digit involved and the status of adjacent joints. After a careful evaluation of the patient's capabilities and needs, it is possible to improve these deformities by the methods discussed.", "contents": "Surgical treatment of the boutonniere deformity in rheumatoid arthritis. The rheumatoid boutonniere deformity does not limit hand function significantly until it becomes severe. For this reason the surgical procedures used should not risk or sacrifice existing function. For the mild cases one can improve the digital balance by a simple extensor tenotomy combined with dynamic splinting. In more advanced stages with passive correction possible, the deformity can be improved by reconstruction of the extensor mechanism. With fixed deformities one can choose between proximal interphalangeal joint fusion and arthroplasty. The decision is influenced by several factors, including the digit involved and the status of adjacent joints. After a careful evaluation of the patient's capabilities and needs, it is possible to improve these deformities by the methods discussed."}
{"id": "PMID:1099509", "title": "Preventive surgery - tenosynovectomy and synovectomy.", "content": "The goal of preventative rheumatoid hand surgery is the eradication of the diseased synovium or tenosynovium before irreparable joint or tendon destruction occurs. Although there is general agreement that both dorsal and flexor tenosynovectomy are reliable and effective procedures to prevent the complications of tenosynovitis, the case for synovectomy is less well established. We have discussed the indications for both tenosynovectomy and synovectomy and described the surgical techniques used for dorsal tenosynovectomy and flexor tenosynovectomy in the wrist, palm, and digits. In addition, the treatment of both extensor and flexor tendon ruptures has been presented.", "contents": "Preventive surgery - tenosynovectomy and synovectomy. The goal of preventative rheumatoid hand surgery is the eradication of the diseased synovium or tenosynovium before irreparable joint or tendon destruction occurs. Although there is general agreement that both dorsal and flexor tenosynovectomy are reliable and effective procedures to prevent the complications of tenosynovitis, the case for synovectomy is less well established. We have discussed the indications for both tenosynovectomy and synovectomy and described the surgical techniques used for dorsal tenosynovectomy and flexor tenosynovectomy in the wrist, palm, and digits. In addition, the treatment of both extensor and flexor tendon ruptures has been presented."}
{"id": "PMID:1099512", "title": "Preoperative and postoperative management: the role of allied health professionals.", "content": "The success of a coordinated team approach is dependent upon respect and communication among all members, all directed toward the individual patient's goal. Standardized forms can be developed by each discipline and used as a formal mode of communication. These can be available in each patient's record for all the professional staff to read. Unitization has established an atomosphere for informal ongoing communication: i.e. allied health team members have been assigned to the same patient care unit. The same allied health members are therefore working together with the same patient on a day by day basis. Greater efficiency and communication tend to develop spontaneously within such a system. Full inpatient coverage seven days a week by the therapist as well as the nurses has improved continuity of care. Since these changes in staff distribution have taken place, a study of a significant number of patients undergoin total knee or toal hip procedures has shown that there has been a reduction in the average length of stay for the hospitalization, from two days for a total knee replacement to five days for a patient undergoing total hip replacement. Weekly or twice weekly meetings of allied health members, surgeons, and rheumatologists greatly facilitate the progression and coorination of the treatment program. Medical and nursing audits on patients with total hip procedures document a remarkably low rate of surgical complications. Early discharge planning has fostered a smooth transition of the patient to his home and ultimately to work.", "contents": "Preoperative and postoperative management: the role of allied health professionals. The success of a coordinated team approach is dependent upon respect and communication among all members, all directed toward the individual patient's goal. Standardized forms can be developed by each discipline and used as a formal mode of communication. These can be available in each patient's record for all the professional staff to read. Unitization has established an atomosphere for informal ongoing communication: i.e. allied health team members have been assigned to the same patient care unit. The same allied health members are therefore working together with the same patient on a day by day basis. Greater efficiency and communication tend to develop spontaneously within such a system. Full inpatient coverage seven days a week by the therapist as well as the nurses has improved continuity of care. Since these changes in staff distribution have taken place, a study of a significant number of patients undergoin total knee or toal hip procedures has shown that there has been a reduction in the average length of stay for the hospitalization, from two days for a total knee replacement to five days for a patient undergoing total hip replacement. Weekly or twice weekly meetings of allied health members, surgeons, and rheumatologists greatly facilitate the progression and coorination of the treatment program. Medical and nursing audits on patients with total hip procedures document a remarkably low rate of surgical complications. Early discharge planning has fostered a smooth transition of the patient to his home and ultimately to work."}
{"id": "PMID:1099521", "title": "Basic and clinical aspects of the alpha1-antitrypsin.", "content": "A deficiency of the major serum alpha1-globulin, the alpha1-antitrypsin, was first described in five patients by Laurell and Eriksson in Sweden in 1963. It soon became obvious that severe alpha1-antitrypsin deficiency was familial, and highly associated with chronic lung disease, having its onset in the third or fourth decade of life. Since the early descriptions of this common deficiency state, it has become clearly associated with familial emphysema in some families, familial infantile cirrhosis in others, and occasionally with a combination of childhood lung and liver disease in siblings. For the pediatrician, severe alpha1-antitrypsin deficiency now enters into the differential diagnosis of both chronic pulmonary disease in childhood and obstructive jaundice in the newborn period; In addition, low levels of alpha1-antitrypsin in serum are characteristic of respiratory distress syndrome, and elevations of this protein may be found in a variety of clinical situations. The, alpha1-antitrypsin probably functions as a major control protein against the tissue-damaging effects of both endogenous and exogenous enzymes. This review will cover several basic and clinical features of this protein with respect to its importance in pediatrics.", "contents": "Basic and clinical aspects of the alpha1-antitrypsin. A deficiency of the major serum alpha1-globulin, the alpha1-antitrypsin, was first described in five patients by Laurell and Eriksson in Sweden in 1963. It soon became obvious that severe alpha1-antitrypsin deficiency was familial, and highly associated with chronic lung disease, having its onset in the third or fourth decade of life. Since the early descriptions of this common deficiency state, it has become clearly associated with familial emphysema in some families, familial infantile cirrhosis in others, and occasionally with a combination of childhood lung and liver disease in siblings. For the pediatrician, severe alpha1-antitrypsin deficiency now enters into the differential diagnosis of both chronic pulmonary disease in childhood and obstructive jaundice in the newborn period; In addition, low levels of alpha1-antitrypsin in serum are characteristic of respiratory distress syndrome, and elevations of this protein may be found in a variety of clinical situations. The, alpha1-antitrypsin probably functions as a major control protein against the tissue-damaging effects of both endogenous and exogenous enzymes. This review will cover several basic and clinical features of this protein with respect to its importance in pediatrics."}
{"id": "PMID:1099522", "title": "The peripheral hemodynamic effects of continuous positive transpulmonary pressure breathing in neonates free from cardiorespiratory disease.", "content": "We applied continuous positive transpulmonary pressure (CPTP) by face mask to 22 spontaneously breathing infants who were free from cardiorespiratory disease, and measured resultant changes in peak esophageal pressure (Pes) and peripheral perfusion (Q1). We measured Pes by balloon and transducer, and Q1 by venous occlusion plethysmography with a mercury-in-rubber strain gauge. Application of 7.6 cm H2O CPTP led to a 13% decrease in Q1 (paired t-test=2.39; P less than .02). Thirty-two percent of the applied CPTP was detected as a change in Pes. The biological significance of a 13% decrease in peripheral perfusion is probably minimal.", "contents": "The peripheral hemodynamic effects of continuous positive transpulmonary pressure breathing in neonates free from cardiorespiratory disease. We applied continuous positive transpulmonary pressure (CPTP) by face mask to 22 spontaneously breathing infants who were free from cardiorespiratory disease, and measured resultant changes in peak esophageal pressure (Pes) and peripheral perfusion (Q1). We measured Pes by balloon and transducer, and Q1 by venous occlusion plethysmography with a mercury-in-rubber strain gauge. Application of 7.6 cm H2O CPTP led to a 13% decrease in Q1 (paired t-test=2.39; P less than .02). Thirty-two percent of the applied CPTP was detected as a change in Pes. The biological significance of a 13% decrease in peripheral perfusion is probably minimal."}
{"id": "PMID:1099523", "title": "The therapeutic application of end-expiratory pressure in the meconium aspiration syndrome.", "content": "The effectiveness of end-expiratory pressure (EEP) in relieving hypoxemia in the meconium aspiration syndrome (MAS) was studied in 14 patients with the disorder. These infants demonstrated a direct, mean PO2 response of 12 torr/cm H2O EEP. A maximum PO2 response was observed in an EEP range of 4 to 7 cm H2O. EEP was equally effective whether patients were breathing spontaneously or were being mechanically ventilated. EEP is useful in the treatment of hypoxemia in the infants with meconium aspiration syndrome.", "contents": "The therapeutic application of end-expiratory pressure in the meconium aspiration syndrome. The effectiveness of end-expiratory pressure (EEP) in relieving hypoxemia in the meconium aspiration syndrome (MAS) was studied in 14 patients with the disorder. These infants demonstrated a direct, mean PO2 response of 12 torr/cm H2O EEP. A maximum PO2 response was observed in an EEP range of 4 to 7 cm H2O. EEP was equally effective whether patients were breathing spontaneously or were being mechanically ventilated. EEP is useful in the treatment of hypoxemia in the infants with meconium aspiration syndrome."}
{"id": "PMID:1099524", "title": "Hyaline membrane disease treated with early nasal end-expiratory pressure: one year's experience.", "content": "This report describes one year's experience treating hyaline membrane disease (HMD) with nasal end-expiratory pressure (NEEP). During the 12 months from July 1, 1973 through June 30, 1974, 119 children with HMD were admitted to the Intensive Care Unit of St. Paul Children's Hospital. Sixty-nine infants were treated early in the course of their disease with NEEP. The survival, incidence of complications, and the number of endotracheal intubations are reported and compared to our experience during a similar time period prior to the use of NEEP (1971-1972). Since the advent of the early application of modest amounts of end-expiratory pressure by nasopharyngeal tube, there has been an increase in the survival of all admissions with HMD, but the increase was statistically significant (P less than .01) only in those weighing 1,501 to 2,000 gm. There was a significant decrease (P less than .025) in the total number of children with HMD requiring endotracheal intubation. There was no change in the incidence of pneumothoraces or bronchopulmonary dysplasia. NEEP is a simple and effective technique for creating continuous airway distending pressure. Its effectiveness and ready availability make the routine endotracheal intubation of infants requiring only continuous airway distending pressure no longer justifiable.", "contents": "Hyaline membrane disease treated with early nasal end-expiratory pressure: one year's experience. This report describes one year's experience treating hyaline membrane disease (HMD) with nasal end-expiratory pressure (NEEP). During the 12 months from July 1, 1973 through June 30, 1974, 119 children with HMD were admitted to the Intensive Care Unit of St. Paul Children's Hospital. Sixty-nine infants were treated early in the course of their disease with NEEP. The survival, incidence of complications, and the number of endotracheal intubations are reported and compared to our experience during a similar time period prior to the use of NEEP (1971-1972). Since the advent of the early application of modest amounts of end-expiratory pressure by nasopharyngeal tube, there has been an increase in the survival of all admissions with HMD, but the increase was statistically significant (P less than .01) only in those weighing 1,501 to 2,000 gm. There was a significant decrease (P less than .025) in the total number of children with HMD requiring endotracheal intubation. There was no change in the incidence of pneumothoraces or bronchopulmonary dysplasia. NEEP is a simple and effective technique for creating continuous airway distending pressure. Its effectiveness and ready availability make the routine endotracheal intubation of infants requiring only continuous airway distending pressure no longer justifiable."}
{"id": "PMID:1099526", "title": "Aspirin intolerance in chronic childhood asthma: Detected by oral challenge.", "content": "Most previously reported individuals with acetylsalicylic acid (aspirin, ASA)-induced asthma have been adults. This study was undertaken to define the prevalence of ASA intolerance among children with intractable asthma. Fifty children (34 boys and 16 girls) ranging in age from 6 to 18 years with extrinsic (atopic) asthma were studied. None had a history of ASA sensitivity or nasal polyps; all required continuous medication including cromolyn sodium and daily or intermittent steroids. Anti-asthmatic medications were stopped 12 hours prior to testing. At the same time of day, each subject, in a doubleblind manner, on two separate days, ingested either 300 mg of ASA or 100 mg of lactose (placebo). Measurements of FVC, FEV1, and FEF25-75 were obtained prior to challenge and one half, one, two, three, and four hours after. ASA intolerance required a decrease of 30% in lung function with ASA as compared to placebo. Fourteen of 50 (28%) were ASA-intolerant; a greater number were girls, and they had more sinusitis and an onset of disease prior to 2 years of age. Steroid dependency, frequency of eczema, nasal eosinophilia, serum IgE levels, and peripheral eosinophil counts did not differ between the two groups. The use of aspirin in childhood asthma should be limited.", "contents": "Aspirin intolerance in chronic childhood asthma: Detected by oral challenge. Most previously reported individuals with acetylsalicylic acid (aspirin, ASA)-induced asthma have been adults. This study was undertaken to define the prevalence of ASA intolerance among children with intractable asthma. Fifty children (34 boys and 16 girls) ranging in age from 6 to 18 years with extrinsic (atopic) asthma were studied. None had a history of ASA sensitivity or nasal polyps; all required continuous medication including cromolyn sodium and daily or intermittent steroids. Anti-asthmatic medications were stopped 12 hours prior to testing. At the same time of day, each subject, in a doubleblind manner, on two separate days, ingested either 300 mg of ASA or 100 mg of lactose (placebo). Measurements of FVC, FEV1, and FEF25-75 were obtained prior to challenge and one half, one, two, three, and four hours after. ASA intolerance required a decrease of 30% in lung function with ASA as compared to placebo. Fourteen of 50 (28%) were ASA-intolerant; a greater number were girls, and they had more sinusitis and an onset of disease prior to 2 years of age. Steroid dependency, frequency of eczema, nasal eosinophilia, serum IgE levels, and peripheral eosinophil counts did not differ between the two groups. The use of aspirin in childhood asthma should be limited."}
{"id": "PMID:1099530", "title": "Apparent motion and the Pulfrich effect.", "content": "The Pulfrich pendulum effect, obtained by viewing a moving object with a filter over one eye, was examined with target stimuli in apparent, rather than continuous, motion. The filter-induced depth effect persisted until a certain degree of intermittency in the presentations of the target was reached, and then it broke down. The degree of intermittency that could be tolerated before the depth effect broke down increased with the density of the filter. It could be argued that the filter determined a shift in the pairing of successive inputs to the eyes, such that the target position in the unfiltered eye was fused with the preceding target position in the filtered eye. However, it appears that the shifted-pairing effect cannot account for the depth impression seen when the target intermittency is less than about 30 ms. Below this value of intermittency a filter can produce a depth effect even when the delay it introduces is small in comparison to the intermittency of the input. The depth effect seen with intermittencies less than 30 ms appears to be of the same magnitude as that obtained with stimuli in continuous motion. It is concluded that a filter can cause two different kinds of depth shift with apparently moving stimuli.", "contents": "Apparent motion and the Pulfrich effect. The Pulfrich pendulum effect, obtained by viewing a moving object with a filter over one eye, was examined with target stimuli in apparent, rather than continuous, motion. The filter-induced depth effect persisted until a certain degree of intermittency in the presentations of the target was reached, and then it broke down. The degree of intermittency that could be tolerated before the depth effect broke down increased with the density of the filter. It could be argued that the filter determined a shift in the pairing of successive inputs to the eyes, such that the target position in the unfiltered eye was fused with the preceding target position in the filtered eye. However, it appears that the shifted-pairing effect cannot account for the depth impression seen when the target intermittency is less than about 30 ms. Below this value of intermittency a filter can produce a depth effect even when the delay it introduces is small in comparison to the intermittency of the input. The depth effect seen with intermittencies less than 30 ms appears to be of the same magnitude as that obtained with stimuli in continuous motion. It is concluded that a filter can cause two different kinds of depth shift with apparently moving stimuli."}
{"id": "PMID:1099532", "title": "[Transuterine migration of intrauterine contraceptive devices. 12 cases].", "content": "Transuterine migration of an IUCD is a rare complication, more spectacular than serious. In general asymptomatic or associated with only minor symptoms, such a perforation usually passes unnoticed. The diagnosis is made when the absence of the string at the cervix is noted at routine examination, and may be proved by radiological examination of the uterine cavity. Treatment consists of removal of the migratory foreign body either by coelioscopy or by laparotomy. Although the no single mechanism for the occurrence of such perforations exists, this study confirmed the high incidence of this complication when the device is inserted in the immediate post-partum period.", "contents": "[Transuterine migration of intrauterine contraceptive devices. 12 cases]. Transuterine migration of an IUCD is a rare complication, more spectacular than serious. In general asymptomatic or associated with only minor symptoms, such a perforation usually passes unnoticed. The diagnosis is made when the absence of the string at the cervix is noted at routine examination, and may be proved by radiological examination of the uterine cavity. Treatment consists of removal of the migratory foreign body either by coelioscopy or by laparotomy. Although the no single mechanism for the occurrence of such perforations exists, this study confirmed the high incidence of this complication when the device is inserted in the immediate post-partum period."}
{"id": "PMID:1099537", "title": "[Detection of hepatic lesions in patients with multiple injuries. Value of the determination of blood glutamo-pyruvic transaminase].", "content": "Serum glutamic pyruvic transaminase (S.G.P.T.) was measured in a series of 39 patients with multiple injuries in order to test its value in detecting liver lesion. An increase above five times the upper limit of the normal value was found in 15 out of the 20 patients having a liver injury and in all of those in whom S.G.P.T. was measured within the first 24 hours following the trauma. In contrast, a similar elevation was observed only in two out of the 19 patients without liver injury. It is suggested that S.G.P.T. determination should be routinely performed in patients with multiple injuries when abdominal signs are poor or concealed by associated lesions. A marked elevation of S.G.P.T. seems in any case suggestive enough to advise a liver arteriogram if hemodynamic conditions allow to perform it.", "contents": "[Detection of hepatic lesions in patients with multiple injuries. Value of the determination of blood glutamo-pyruvic transaminase]. Serum glutamic pyruvic transaminase (S.G.P.T.) was measured in a series of 39 patients with multiple injuries in order to test its value in detecting liver lesion. An increase above five times the upper limit of the normal value was found in 15 out of the 20 patients having a liver injury and in all of those in whom S.G.P.T. was measured within the first 24 hours following the trauma. In contrast, a similar elevation was observed only in two out of the 19 patients without liver injury. It is suggested that S.G.P.T. determination should be routinely performed in patients with multiple injuries when abdominal signs are poor or concealed by associated lesions. A marked elevation of S.G.P.T. seems in any case suggestive enough to advise a liver arteriogram if hemodynamic conditions allow to perform it."}
{"id": "PMID:1099531", "title": "[Influence of restrictive use of antibiotics on the development of drug resistance in intestinal Escherichia coli from pigs (author's transl)].", "content": "A retrospective investigation into the drug resistance pattern of intestinal E. coli was carried out in 17 closely monitored herds in order to evaluate a possible influence of an improved diagnostic and a restrictive use of antibiotics in swine herds. The herds participated from October, 1970, in an investigation of preweaning mortality and morbidity, and during a two-year investigation period antibiotics were only used when therapeutically indicated. Sensitivity tests were regularly performed on the intestinal flora from post mortem examined pigs from the herds and the results forwarded to the local veterinarians. A total of 443 E. Coli strains were isolated in pure or almost pure culture from the  jejunum of post mortem examined pigs and tested for sensitivity, using the following antibiotics: streptomycin, compound sulphonamide, neomycin, oxytetracycline, chloramphenicol, nalidixic acid, furazolidone, and ampicillin. During the investigation period, the frequency of isolated multiresistant E. coli strains decreazed from 67.7 per cent to 9.5 per cent and the number of strains without antibiotic resistance increased from 3.0 per cent to 36.2 per cent (Table I; Fig. 1). Herds were divided into 3 groups according to different intensity of antibiotic administration and to closed or open management system (Table II). The most pronounced decrease in the number of multiresistant E. coli strains (from 64.9 to 2.0 per cent) was observed in Group I, which comprises closed herds with a rapid decrease in the use of antibiotics. In Group II, which comprises semi-closed herds with a decreasing, but in the last year of the investigation still frequent use of antibiotics, a somewhat slower decrease of the number of multiresistant strains to a level of 12.1 per cent was observed. The number of strains without resistance increased to approximately the same level as in Group I. In Group III, which comprises open herds with infrequent use of antibiotics, the incidence of resistant E. coli strains fluctuated (Table III; Fig. 2). The resistance was proved transferable in 60.5 per cent of the examined resistant strains. The frequency of transferability did not show any significant change during the investigation period (Table IV). The most frequently encountered resistance determinants were: sulphonamide (97 per cent), streptomycin (40 per cent), and oxytetracycline (34 per cent). Only resistance against sulphonamide, streptomycin, tetracycline, and chloramphenicol was proved transferable (Table V). It is concluded that it is possible to obtain a simultaneous reduction of induced antibiotic resistance of intestinal E. coli and of enteric diseases in sucking pigs in commercial herds, by adjustment of management and rational use of antibiotics based on improved diagnostic including in vitro sensitivity tests.", "contents": "[Influence of restrictive use of antibiotics on the development of drug resistance in intestinal Escherichia coli from pigs (author's transl)]. A retrospective investigation into the drug resistance pattern of intestinal E. coli was carried out in 17 closely monitored herds in order to evaluate a possible influence of an improved diagnostic and a restrictive use of antibiotics in swine herds. The herds participated from October, 1970, in an investigation of preweaning mortality and morbidity, and during a two-year investigation period antibiotics were only used when therapeutically indicated. Sensitivity tests were regularly performed on the intestinal flora from post mortem examined pigs from the herds and the results forwarded to the local veterinarians. A total of 443 E. Coli strains were isolated in pure or almost pure culture from the  jejunum of post mortem examined pigs and tested for sensitivity, using the following antibiotics: streptomycin, compound sulphonamide, neomycin, oxytetracycline, chloramphenicol, nalidixic acid, furazolidone, and ampicillin. During the investigation period, the frequency of isolated multiresistant E. coli strains decreazed from 67.7 per cent to 9.5 per cent and the number of strains without antibiotic resistance increased from 3.0 per cent to 36.2 per cent (Table I; Fig. 1). Herds were divided into 3 groups according to different intensity of antibiotic administration and to closed or open management system (Table II). The most pronounced decrease in the number of multiresistant E. coli strains (from 64.9 to 2.0 per cent) was observed in Group I, which comprises closed herds with a rapid decrease in the use of antibiotics. In Group II, which comprises semi-closed herds with a decreasing, but in the last year of the investigation still frequent use of antibiotics, a somewhat slower decrease of the number of multiresistant strains to a level of 12.1 per cent was observed. The number of strains without resistance increased to approximately the same level as in Group I. In Group III, which comprises open herds with infrequent use of antibiotics, the incidence of resistant E. coli strains fluctuated (Table III; Fig. 2). The resistance was proved transferable in 60.5 per cent of the examined resistant strains. The frequency of transferability did not show any significant change during the investigation period (Table IV). The most frequently encountered resistance determinants were: sulphonamide (97 per cent), streptomycin (40 per cent), and oxytetracycline (34 per cent). Only resistance against sulphonamide, streptomycin, tetracycline, and chloramphenicol was proved transferable (Table V). It is concluded that it is possible to obtain a simultaneous reduction of induced antibiotic resistance of intestinal E. coli and of enteric diseases in sucking pigs in commercial herds, by adjustment of management and rational use of antibiotics based on improved diagnostic including in vitro sensitivity tests."}
{"id": "PMID:1099542", "title": "[Tests of stimulation of calcitonin secretion. Value in medullary cancer of the thyroid].", "content": "The detection of high circulating levels of calcitonin is a most valuable procedure to diagnose advanced cases of medullary carcinoma of the thyroid. However, the diagnosis of a primitive tumor in the early stages of development (as in cases of the familial form of the disease) or of a metastasis following ablation of the tumor is more difficult. In the latter cases, the levels of circulating calcitonin may be within normal limits, and for diagnosis one must then resort to tests to stimulate the secretion of calcitonin. We are reporting from our personal experiences the advantages, disadvantages and inconveniences of the three most well-known tests. All patients responded positively to administration of calcium or pentagastrin. The alcohol test, however, produced inconsistent results.", "contents": "[Tests of stimulation of calcitonin secretion. Value in medullary cancer of the thyroid]. The detection of high circulating levels of calcitonin is a most valuable procedure to diagnose advanced cases of medullary carcinoma of the thyroid. However, the diagnosis of a primitive tumor in the early stages of development (as in cases of the familial form of the disease) or of a metastasis following ablation of the tumor is more difficult. In the latter cases, the levels of circulating calcitonin may be within normal limits, and for diagnosis one must then resort to tests to stimulate the secretion of calcitonin. We are reporting from our personal experiences the advantages, disadvantages and inconveniences of the three most well-known tests. All patients responded positively to administration of calcium or pentagastrin. The alcohol test, however, produced inconsistent results."}
{"id": "PMID:1099566", "title": "Problems of aging: diagnosing and treating leg pain due to arteriosclerosis obliterans.", "content": "Management of patients with arteriosclerosis obliterans begins with organization of the history, results of physical examination, and blood flow measurements into a patient profile. This approach permits the practicing physician to monitor the progression of the obstructive process over time. With close observation, a controlled exercise program, and properly timed surgical intervention, if necessary, patients with arterioslcerosis obliterans can enjoy a full and productive life.", "contents": "Problems of aging: diagnosing and treating leg pain due to arteriosclerosis obliterans. Management of patients with arteriosclerosis obliterans begins with organization of the history, results of physical examination, and blood flow measurements into a patient profile. This approach permits the practicing physician to monitor the progression of the obstructive process over time. With close observation, a controlled exercise program, and properly timed surgical intervention, if necessary, patients with arterioslcerosis obliterans can enjoy a full and productive life."}
{"id": "PMID:1099567", "title": "Pediatric ophthalmic mythology.", "content": "Headaches in children are virtually never the result of eye problems. If a child needs glasses, young age is almost never a contraindication to their use. Any child in whom strabismus is recognized or suspected should be referred to an ophthalmologist. The strabismus may be a presenting sign of a serious disease. Even if it is not, spontaneous cure almost never occurs. Furthermore, the younger the child, the easier and more successful will be the treatment of both the strabismus and the amblyopia that often accompanies it. Dyslexia is not the result of abnormalities of the eyes and is unaffected by eye exercises. There are no significant ocular hazards in the use of standard mydriatic (dilating) eye drops in children.", "contents": "Pediatric ophthalmic mythology. Headaches in children are virtually never the result of eye problems. If a child needs glasses, young age is almost never a contraindication to their use. Any child in whom strabismus is recognized or suspected should be referred to an ophthalmologist. The strabismus may be a presenting sign of a serious disease. Even if it is not, spontaneous cure almost never occurs. Furthermore, the younger the child, the easier and more successful will be the treatment of both the strabismus and the amblyopia that often accompanies it. Dyslexia is not the result of abnormalities of the eyes and is unaffected by eye exercises. There are no significant ocular hazards in the use of standard mydriatic (dilating) eye drops in children."}
{"id": "PMID:1099568", "title": "Recognizing the allergic individual.", "content": "Patients with respiratory tract allergy present certain auditory and visual signs which, when correlated with the history, aid substantially in arriving at a conclusive diagnosis. The physician should be alert to the signs that indicate allergic disease. Early recognition followed by modern allergy management will help to prevent progression of the allergic process.", "contents": "Recognizing the allergic individual. Patients with respiratory tract allergy present certain auditory and visual signs which, when correlated with the history, aid substantially in arriving at a conclusive diagnosis. The physician should be alert to the signs that indicate allergic disease. Early recognition followed by modern allergy management will help to prevent progression of the allergic process."}
{"id": "PMID:1099571", "title": "Vitamin E or vitamin A protects chickens against E. coli infection.", "content": "The supplementation of either vitamin E (300 mg./kg. diet) or vitamin A (60,000 I.U./kg. diet) to a standard chick ration increased the protection of six week old immunized chickens against E coli infection, decreasing mortality from about 40% to 5%. The combination of the two vitamins, however, did not give as much protection as either vitamin alone. Vitamin E or A did not protect chicks from weight loss and severe morbidity due to infection, but slightly increased the rate of recovery.", "contents": "Vitamin E or vitamin A protects chickens against E. coli infection. The supplementation of either vitamin E (300 mg./kg. diet) or vitamin A (60,000 I.U./kg. diet) to a standard chick ration increased the protection of six week old immunized chickens against E coli infection, decreasing mortality from about 40% to 5%. The combination of the two vitamins, however, did not give as much protection as either vitamin alone. Vitamin E or A did not protect chicks from weight loss and severe morbidity due to infection, but slightly increased the rate of recovery."}
{"id": "PMID:1099579", "title": "Repetitive DNA replication of the incomplete genomes of phage T4 petite particles.", "content": "The genomes of petite T4 phage particles presumably cannot circularize because they are deficient for a significant terminal segment and hence not terminally redundant like normal T4 genomes. Combined density- and 32P-labeling shows that the majority of such deficient DNA molecules can nevertheless replicate their entire length. Furthermore, the density-shift technique shows that replicated parental strands can exchange their partners for new light strands, indicating that noncircularized T4 DNA molecules replicate repeatedly. When taken together with previously published data, these results indicate that T4 replication is bidirectional from multiple, genetically fixed points of origin. Rolling circle models can, therefore, not be considered as an essential mechanism for the early rounds of T4 replication.", "contents": "Repetitive DNA replication of the incomplete genomes of phage T4 petite particles. The genomes of petite T4 phage particles presumably cannot circularize because they are deficient for a significant terminal segment and hence not terminally redundant like normal T4 genomes. Combined density- and 32P-labeling shows that the majority of such deficient DNA molecules can nevertheless replicate their entire length. Furthermore, the density-shift technique shows that replicated parental strands can exchange their partners for new light strands, indicating that noncircularized T4 DNA molecules replicate repeatedly. When taken together with previously published data, these results indicate that T4 replication is bidirectional from multiple, genetically fixed points of origin. Rolling circle models can, therefore, not be considered as an essential mechanism for the early rounds of T4 replication."}
{"id": "PMID:1099580", "title": "Reversible, coenzyme-A-mediated inactivation of biosynthetic condensing enzymes in yeast: a possible regulatory mechanism.", "content": "alpha-Isopropylmalate synthase [3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methylbutyrate-lyase (CoA-acetylating); EC 4.1.3.12], the enzyme catalyzing the first committed step in leucine biosynthesis, and homocitrate synthase [3-hydroxy-3-carboxyadipate 2-oxoglutarate-lyase (CoA-acetylating); EC 4.1.3.21], the first enzyme in lysine biosynthesis in yeast, are rapidly inactivated in the presence of low concentrations of coenzyme A, a product of both reactions. Closely related compounds like 3-dephospho-coenzyme A or oxidized coenzyme A are almost without effect, as are other sulfhydryl compounds. Citrate (si)-synthase [citrate oxaloacetate-lyase (pro-3S-CH2-COO-minus leads to acetyl-CoA); EC 4.1.3.7] appears to be completely resistant against inactivation by coenzyme A. Inactivated alpha-isopropylmalate and homocitrate synthases can be reactivated by dialysis, but not by adding excess substrate. Protection against coenzyme-A-mediated inactivation is provided by relatively high concentrations of the alpha-ketoacid substrate or the specific end product inhibitor of each of the two enzymes. The coenzyme-A-mediated inactivation of alpha-isopropylmalate synthase has been more closely investigated. It requires the presence of divalent metal ions, with Zn++being most effective. The inactivation does not require molecular oxygen. It occurs in the presence of low concentrations of substrates and is observed in toluene-treated cells. These results, together with evidence that alpha-isopropylmalate synthase and homocitrate synthase are located in the mitochondria, suggest a mechanism by which increasing intra-mitochondrial coenzyme A concentrations might serve as a signal of decreasing acetyl-coenzyme A levels, triggering a temporary inactivation of biosynthetic acetyl-coenzyme A-consuming reactions in order to channel the available acetyl-coenzyme A into the citrate cycle.", "contents": "Reversible, coenzyme-A-mediated inactivation of biosynthetic condensing enzymes in yeast: a possible regulatory mechanism. alpha-Isopropylmalate synthase [3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methylbutyrate-lyase (CoA-acetylating); EC 4.1.3.12], the enzyme catalyzing the first committed step in leucine biosynthesis, and homocitrate synthase [3-hydroxy-3-carboxyadipate 2-oxoglutarate-lyase (CoA-acetylating); EC 4.1.3.21], the first enzyme in lysine biosynthesis in yeast, are rapidly inactivated in the presence of low concentrations of coenzyme A, a product of both reactions. Closely related compounds like 3-dephospho-coenzyme A or oxidized coenzyme A are almost without effect, as are other sulfhydryl compounds. Citrate (si)-synthase [citrate oxaloacetate-lyase (pro-3S-CH2-COO-minus leads to acetyl-CoA); EC 4.1.3.7] appears to be completely resistant against inactivation by coenzyme A. Inactivated alpha-isopropylmalate and homocitrate synthases can be reactivated by dialysis, but not by adding excess substrate. Protection against coenzyme-A-mediated inactivation is provided by relatively high concentrations of the alpha-ketoacid substrate or the specific end product inhibitor of each of the two enzymes. The coenzyme-A-mediated inactivation of alpha-isopropylmalate synthase has been more closely investigated. It requires the presence of divalent metal ions, with Zn++being most effective. The inactivation does not require molecular oxygen. It occurs in the presence of low concentrations of substrates and is observed in toluene-treated cells. These results, together with evidence that alpha-isopropylmalate synthase and homocitrate synthase are located in the mitochondria, suggest a mechanism by which increasing intra-mitochondrial coenzyme A concentrations might serve as a signal of decreasing acetyl-coenzyme A levels, triggering a temporary inactivation of biosynthetic acetyl-coenzyme A-consuming reactions in order to channel the available acetyl-coenzyme A into the citrate cycle."}
{"id": "PMID:1099583", "title": "On becoming a confederation.", "content": "This paper was presented as the keynote speech at the Seventh International Congress of the World Confederation for Physical Therapy. The question of what it will take to strengthen physical therapy as a world force for the health of mankind is examined. The past and present status of physical therapy and the Confederation are assessed as background for looking at ways to help the Confederation become truly a World Confederation for Physical Therapy.", "contents": "On becoming a confederation. This paper was presented as the keynote speech at the Seventh International Congress of the World Confederation for Physical Therapy. The question of what it will take to strengthen physical therapy as a world force for the health of mankind is examined. The past and present status of physical therapy and the Confederation are assessed as background for looking at ways to help the Confederation become truly a World Confederation for Physical Therapy."}
{"id": "PMID:1099585", "title": "Technique for vestibular neurotomy in the rat.", "content": "A technique for vestibular neurotomy in the rat has been developed. Removal of the tympanic membrane and ossicles through the external auditory meatus permits access to the auditory bulla. After the pterygopalatine artery has been cauterized, and the electrode may be introduced through the oval widow. The vestibular branch of the VIII nerve can then be cauterized with the electrode without direct visualization.", "contents": "Technique for vestibular neurotomy in the rat. A technique for vestibular neurotomy in the rat has been developed. Removal of the tympanic membrane and ossicles through the external auditory meatus permits access to the auditory bulla. After the pterygopalatine artery has been cauterized, and the electrode may be introduced through the oval widow. The vestibular branch of the VIII nerve can then be cauterized with the electrode without direct visualization."}
{"id": "PMID:1099586", "title": "Chemistor: a reusable telestimulation device for direct chemical stimulation of the nonhuman primate brain in freely moving animals.", "content": "A reusable telemetric multi-injector unit (chemistor) suitable for injections into the brain of primates has been developed and tested in chronic experiments with rhesus monkeys freely moving and interacting with other monkeys. The chemistor unit, which measures 15.0 by 15.0 by 7.5 mm and weight 5.8 g, consists of a microinjector, equipped with an internal mechanical power source, and an electronic circuit connected with a receiving coil; a total volume of 27.0 mul to be injected in quanta of 0.9 mul at freely chosen time intervals makes this unit suitable for 30 injections. The desirable protection against possible damage is provided by a circular, head-mounted container (dia. 43.0 mm and 10.3 mm high) which leaves place for two complete chemistors. The device is small, light in weight, reliable and resistant to physical damage.", "contents": "Chemistor: a reusable telestimulation device for direct chemical stimulation of the nonhuman primate brain in freely moving animals. A reusable telemetric multi-injector unit (chemistor) suitable for injections into the brain of primates has been developed and tested in chronic experiments with rhesus monkeys freely moving and interacting with other monkeys. The chemistor unit, which measures 15.0 by 15.0 by 7.5 mm and weight 5.8 g, consists of a microinjector, equipped with an internal mechanical power source, and an electronic circuit connected with a receiving coil; a total volume of 27.0 mul to be injected in quanta of 0.9 mul at freely chosen time intervals makes this unit suitable for 30 injections. The desirable protection against possible damage is provided by a circular, head-mounted container (dia. 43.0 mm and 10.3 mm high) which leaves place for two complete chemistors. The device is small, light in weight, reliable and resistant to physical damage."}
{"id": "PMID:1099587", "title": "Inexpensive stereotaxic sighting microscope.", "content": "An inexpensive low-power microscope is described for use in stereotaxic surgery. The microscope aids in the measurement of skull landmarks and improves the accuracy of stereotaxic surgery.", "contents": "Inexpensive stereotaxic sighting microscope. An inexpensive low-power microscope is described for use in stereotaxic surgery. The microscope aids in the measurement of skull landmarks and improves the accuracy of stereotaxic surgery."}
{"id": "PMID:1099597", "title": "Some problems and potentialities of present-day psychoanalysis.", "content": "In a critical review of the current historical situation of psychoanalysis and its unique problems, the author considers a variety of specific but interacting factors. The potentialities for advance as therapy and science, their interrelationship, and the possible channels for such advance are examined. The social, clinical, and scientific importance of derivative 'psychotherapies' and of rationally flexible boundaries of 'indication' are discussed. As differentiated from rational conservatism, the phenomenon of analysts' irrational resistance to potential change in basic tenets of technique or theory is held to be a critically formidable impediment to the further development of psychoanalysis.", "contents": "Some problems and potentialities of present-day psychoanalysis. In a critical review of the current historical situation of psychoanalysis and its unique problems, the author considers a variety of specific but interacting factors. The potentialities for advance as therapy and science, their interrelationship, and the possible channels for such advance are examined. The social, clinical, and scientific importance of derivative 'psychotherapies' and of rationally flexible boundaries of 'indication' are discussed. As differentiated from rational conservatism, the phenomenon of analysts' irrational resistance to potential change in basic tenets of technique or theory is held to be a critically formidable impediment to the further development of psychoanalysis."}
{"id": "PMID:1099598", "title": "Activity in chronic schizophrenic patients: comparison of pimozide with fluphenazine in a double blind trial.", "content": "A double-blind crossover trial was carried out comparing pimozide with fluphenazine.  The effects of the drugs on the activity of 20 chronic inert male schizophrenic patients were measured using nursing observations, psychiatric rating, and an operant conditioning method.  When activity was assessed in these different ways, no significant difference was found between the two drugs on any of the measures.  It is concluded that pimozide does not effectively increase the activity in such patients.  It is considered that the free operant conditioning method used was shown to be a useful measure for comparing therapy in chronic schizophrenic patients.", "contents": "Activity in chronic schizophrenic patients: comparison of pimozide with fluphenazine in a double blind trial. A double-blind crossover trial was carried out comparing pimozide with fluphenazine.  The effects of the drugs on the activity of 20 chronic inert male schizophrenic patients were measured using nursing observations, psychiatric rating, and an operant conditioning method.  When activity was assessed in these different ways, no significant difference was found between the two drugs on any of the measures.  It is concluded that pimozide does not effectively increase the activity in such patients.  It is considered that the free operant conditioning method used was shown to be a useful measure for comparing therapy in chronic schizophrenic patients."}
{"id": "PMID:1099605", "title": "Neuropharmacological studies on D145 (1,3-dimethyl-5-aminoadamantan).", "content": "The effects of D145 (1,3-dimethyl-5-aminoadamantan) and amantadine on dopaminergic mechanisms in the rat were investigated by use of pharmacological agents known to disrupt dopamine function, by placing electrolytic lesions in the dopamine-containing areas of the extrapyrimidal and mesolimbic systems and by the direct application to dopamine sensitive areas. Stereotypy and circling behaviour were used as behavioural indices of dopaminergic stimulation and apomorphine and d-amphetamine were used as standard dopaminergic agonists. In addition, the possible importance of 5-hydroxytryptamine to the dopamine effects was investigated using electrolytic lesions of the midbrain raph\u00e9 nuclei. Both D145 and amantadine caused a stereotyped behaviour characterized by periodic sniffing, repetitive limb movements and biting, but the effect of amantadine was far more periodic. In addition D145, but not amantadine, caused marked hyperactivity. These behavioural effects were resistant to pretreatment with alpha-methylparatyrosine but not to combined alpha-methylparatyrosine/reserpine or low doses of haloperidol. Also, the prior administration of D145 or amantadine inhibited the development of the biting components of apomorphine and d-amphetamine stereotypy. Both D145 and amantadine caused circling behaviour in animals with asymmetric lesions of the medial raph\u00e9 nucleus or unilateral lesions of the substantia nigra but the action of D145 was more intense. Bilateral electrolytic lesions placed in the extrapyrimidal (caudate-putamen, globus pallidus, substantia nigra), mesolimbic (nucleus accumbens septi, tuberculum olfactorium, nucleus interstitialis stria terminalis, nucleus amygdaloideus centralis) nuclei or the neuronal pathways supplying them showed D145 and amantadine to act in both areas although their action on the extrapyrimidal system was most marked. However, of particular note was the significantly greater involvement of the substantia nigra with the D145 effect, and the greater involvement of the D145 effect with mesolimbic function. Lesions placed in the medial and/or dorsal raph\u00e9 nucleus indicated some involvement of 5-hydroxytryptamine with the actions of both D145 and amantadine. The bilateral intrastriatal application of D145 or amantadine in nialamide pretreated animals failed to induce stereotyped or hyperactive behaviour although contralateral asymmetries, which were abolished by lesions of the substantia nigra, were recorded following the unilateral intrastriatal application of D145 or amantadine in haloperidol pretreated animals.", "contents": "Neuropharmacological studies on D145 (1,3-dimethyl-5-aminoadamantan). The effects of D145 (1,3-dimethyl-5-aminoadamantan) and amantadine on dopaminergic mechanisms in the rat were investigated by use of pharmacological agents known to disrupt dopamine function, by placing electrolytic lesions in the dopamine-containing areas of the extrapyrimidal and mesolimbic systems and by the direct application to dopamine sensitive areas. Stereotypy and circling behaviour were used as behavioural indices of dopaminergic stimulation and apomorphine and d-amphetamine were used as standard dopaminergic agonists. In addition, the possible importance of 5-hydroxytryptamine to the dopamine effects was investigated using electrolytic lesions of the midbrain raph\u00e9 nuclei. Both D145 and amantadine caused a stereotyped behaviour characterized by periodic sniffing, repetitive limb movements and biting, but the effect of amantadine was far more periodic. In addition D145, but not amantadine, caused marked hyperactivity. These behavioural effects were resistant to pretreatment with alpha-methylparatyrosine but not to combined alpha-methylparatyrosine/reserpine or low doses of haloperidol. Also, the prior administration of D145 or amantadine inhibited the development of the biting components of apomorphine and d-amphetamine stereotypy. Both D145 and amantadine caused circling behaviour in animals with asymmetric lesions of the medial raph\u00e9 nucleus or unilateral lesions of the substantia nigra but the action of D145 was more intense. Bilateral electrolytic lesions placed in the extrapyrimidal (caudate-putamen, globus pallidus, substantia nigra), mesolimbic (nucleus accumbens septi, tuberculum olfactorium, nucleus interstitialis stria terminalis, nucleus amygdaloideus centralis) nuclei or the neuronal pathways supplying them showed D145 and amantadine to act in both areas although their action on the extrapyrimidal system was most marked. However, of particular note was the significantly greater involvement of the substantia nigra with the D145 effect, and the greater involvement of the D145 effect with mesolimbic function. Lesions placed in the medial and/or dorsal raph\u00e9 nucleus indicated some involvement of 5-hydroxytryptamine with the actions of both D145 and amantadine. The bilateral intrastriatal application of D145 or amantadine in nialamide pretreated animals failed to induce stereotyped or hyperactive behaviour although contralateral asymmetries, which were abolished by lesions of the substantia nigra, were recorded following the unilateral intrastriatal application of D145 or amantadine in haloperidol pretreated animals."}
{"id": "PMID:1099606", "title": "Psychotropic drugs and impairment of psychomotor functions.", "content": "The present work deals with the effects of psychotropic drug therapy on the operation of psychomotor functions used in a clinical examination of suspected drunken drivers. 100 psychiatric mental, but otherwise health, patients were examined; the type of medication and the number of drugs used varied greatly. In 71 cases the mean degree of error in the clinical examination was higher, and, in several of these, markedly higher than the reference values obtained earlier on suspected drunken drivers when the blood contained very small amounts of alcohol or none at all. In 18 cases coarsely-divided nystagmus was registered in patients on psychotropes. This is an obvious sign of a marked side-effect of medication but was present more infrequently than in subjects with after ingestion of alcohol. The present results indicate that application of the clinical examination method, which was originally developed for and related to the examination of alcohol cases, to subjects on psychotropes is adequate, and it is possible with clinical examination to obtain valuable medicolegal information on the impairment of physiological functions. The present review of suspected drugged drivers examined in Helsinki in 1969--1972 also supports this view.", "contents": "Psychotropic drugs and impairment of psychomotor functions. The present work deals with the effects of psychotropic drug therapy on the operation of psychomotor functions used in a clinical examination of suspected drunken drivers. 100 psychiatric mental, but otherwise health, patients were examined; the type of medication and the number of drugs used varied greatly. In 71 cases the mean degree of error in the clinical examination was higher, and, in several of these, markedly higher than the reference values obtained earlier on suspected drunken drivers when the blood contained very small amounts of alcohol or none at all. In 18 cases coarsely-divided nystagmus was registered in patients on psychotropes. This is an obvious sign of a marked side-effect of medication but was present more infrequently than in subjects with after ingestion of alcohol. The present results indicate that application of the clinical examination method, which was originally developed for and related to the examination of alcohol cases, to subjects on psychotropes is adequate, and it is possible with clinical examination to obtain valuable medicolegal information on the impairment of physiological functions. The present review of suspected drugged drivers examined in Helsinki in 1969--1972 also supports this view."}
{"id": "PMID:1099608", "title": "Changes in human tissue donor attitudes: 1969-1974.", "content": "Surveys of various community groups reveals changes in attitudes regarding human tissue donation from 1969 to the present.  An overall donor ratio of 67% in 1969 is compared to a donor volunteer ratio of 78% in 1974.  Both prospective donors and nondonors rate various organ transplantation procedures more favorably now than in 1969, with nondonors showing a particularly striking rise in evaluation of such procedures.  Even transplant operations such as liver or heart, now largely abandoned, are viewed more favorably today.  Attitudes concerning giving or receiving of blood occupy a special place in donor regard since both donors and nondonors overwhelmingly favor blood transfusion and do not differ significantly in this respect.", "contents": "Changes in human tissue donor attitudes: 1969-1974. Surveys of various community groups reveals changes in attitudes regarding human tissue donation from 1969 to the present.  An overall donor ratio of 67% in 1969 is compared to a donor volunteer ratio of 78% in 1974.  Both prospective donors and nondonors rate various organ transplantation procedures more favorably now than in 1969, with nondonors showing a particularly striking rise in evaluation of such procedures.  Even transplant operations such as liver or heart, now largely abandoned, are viewed more favorably today.  Attitudes concerning giving or receiving of blood occupy a special place in donor regard since both donors and nondonors overwhelmingly favor blood transfusion and do not differ significantly in this respect."}
{"id": "PMID:1099614", "title": "Gastrointestinal complications following renal transplantation.", "content": "Gastrointestinal complications developed in 29 (6%) of 510 patients following renal transplantation. Thirteen patients (45%) died as a result. Gastrointestinal bleeding, usually considered the commonest complication, was seen in only 7 cases. Other complications included pancreatitis and hyperamylasemia, massive ileus, fistulas with abscesses, bowel infarction, peptic ulcers without bleeding, obstruction, gangrenous cholecystitis, esophagitis, spontaneous perforation of the sigmoid colon, and pneumatosis cystoides intestinalis. Six patients had no clinical or laboratory findings suggesting the underlying gastrointestinal complication; it was the radiographic findings that called attention to the acute problem.", "contents": "Gastrointestinal complications following renal transplantation. Gastrointestinal complications developed in 29 (6%) of 510 patients following renal transplantation. Thirteen patients (45%) died as a result. Gastrointestinal bleeding, usually considered the commonest complication, was seen in only 7 cases. Other complications included pancreatitis and hyperamylasemia, massive ileus, fistulas with abscesses, bowel infarction, peptic ulcers without bleeding, obstruction, gangrenous cholecystitis, esophagitis, spontaneous perforation of the sigmoid colon, and pneumatosis cystoides intestinalis. Six patients had no clinical or laboratory findings suggesting the underlying gastrointestinal complication; it was the radiographic findings that called attention to the acute problem."}
{"id": "PMID:1099615", "title": "Regulation of coronary blood flow.", "content": "A very schematic summary of possible factors that affect coronary vascular resistance is illustrated in Fig. 11. These factors are mechanical, myogenic, metabolic, and neural. Mechanical influence is passive and is essentially determined by the extravascular compression which is important only during systole and particularly in the subendocardial layers of the left ventricular myocardium. With respect to the myogenic factor it is controversial whether it plays a role in adjustments of coronary vascular tone. Metabolic processes are possibly integrated at the level of the myocardial cell PO2 which is the resultant of oxygen supply and need, which in turn depend on the myocardial contractile activity as well as other biochemical processes. A decrease in myocardial PO2 gives rise to the release of the vasodilator metabolite adenosine. Nevertheless, other chemical factors (H+, K+, osmolarity) known to be released by the heart (which by themselves are poor and transient coronary vasodilators and whose release does not correspond with changes in coronary resistance) may play a role by modulating the coronary sensitivity to adenosine and other factors.", "contents": "Regulation of coronary blood flow. A very schematic summary of possible factors that affect coronary vascular resistance is illustrated in Fig. 11. These factors are mechanical, myogenic, metabolic, and neural. Mechanical influence is passive and is essentially determined by the extravascular compression which is important only during systole and particularly in the subendocardial layers of the left ventricular myocardium. With respect to the myogenic factor it is controversial whether it plays a role in adjustments of coronary vascular tone. Metabolic processes are possibly integrated at the level of the myocardial cell PO2 which is the resultant of oxygen supply and need, which in turn depend on the myocardial contractile activity as well as other biochemical processes. A decrease in myocardial PO2 gives rise to the release of the vasodilator metabolite adenosine. Nevertheless, other chemical factors (H+, K+, osmolarity) known to be released by the heart (which by themselves are poor and transient coronary vasodilators and whose release does not correspond with changes in coronary resistance) may play a role by modulating the coronary sensitivity to adenosine and other factors."}
{"id": "PMID:1099642", "title": "Chymoral in the treatment of lumbar disc prolapse.", "content": "Ninety-three patients with lumbar disc prolapse of less than six months duration were treated with Chymoral or an identical placebo in a double-blind study. Those patients given Chymoral exhibited significantly greater improvement of straight-leg raising and received significantly fewer analgesics. However, no difference was apparent with regard to other parameters, and similar numbers from both groups required further therapy. Over-all, the value of Chymoral in the treatment of lumbar disc prolapse seemed little better than a placebo.", "contents": "Chymoral in the treatment of lumbar disc prolapse. Ninety-three patients with lumbar disc prolapse of less than six months duration were treated with Chymoral or an identical placebo in a double-blind study. Those patients given Chymoral exhibited significantly greater improvement of straight-leg raising and received significantly fewer analgesics. However, no difference was apparent with regard to other parameters, and similar numbers from both groups required further therapy. Over-all, the value of Chymoral in the treatment of lumbar disc prolapse seemed little better than a placebo."}
{"id": "PMID:1099644", "title": "[Surgical and prosthetic approach to the difficulties of fitting in the completely edentulous].", "content": "Prosthetic Surgery must not be considered as an exceptional process. It is likely to transform the comfort and stability of a complete prosthesis if the indications are well laid down. Two elements combine towards its success. 1. The surgical action, which may be -- a limited vestibuloplasty, destined to abolish the small ligaments, the hyperplasias or the polypoid formations; --an extensive vestibuloplasty, intended to deepen the non-retentive vestibules. The process of choice is a super-periosteal plasty of the CLARKE or VRASSE and BATAILLE type. It may be used above all on the mandible. For the maxilla, CELESNIK's intervention may be associated with it; --free skin grafts. They do not always give the results anticipated because of the cicatrical retraction which is almost constantly observed. 2. The retention is as important as the surgery. It is carried out by an anchorage with wires, in a hammock or transcutaneous, and by a plate of resin lined with a layer of elastomeres of silicon. The material appears very much superior to the sponges of polyvinylic alcohol advocated by VRASSE and NETTER.", "contents": "[Surgical and prosthetic approach to the difficulties of fitting in the completely edentulous]. Prosthetic Surgery must not be considered as an exceptional process. It is likely to transform the comfort and stability of a complete prosthesis if the indications are well laid down. Two elements combine towards its success. 1. The surgical action, which may be -- a limited vestibuloplasty, destined to abolish the small ligaments, the hyperplasias or the polypoid formations; --an extensive vestibuloplasty, intended to deepen the non-retentive vestibules. The process of choice is a super-periosteal plasty of the CLARKE or VRASSE and BATAILLE type. It may be used above all on the mandible. For the maxilla, CELESNIK's intervention may be associated with it; --free skin grafts. They do not always give the results anticipated because of the cicatrical retraction which is almost constantly observed. 2. The retention is as important as the surgery. It is carried out by an anchorage with wires, in a hammock or transcutaneous, and by a plate of resin lined with a layer of elastomeres of silicon. The material appears very much superior to the sponges of polyvinylic alcohol advocated by VRASSE and NETTER."}
{"id": "PMID:1099645", "title": "Laminate-plasty reconstruction of partial auricular defects A new method.", "content": "The problems surrounding the reconstruction of partial defects of the external ear are examined, and a new method is presented for the reconstruction of defects of the helix where up to one-half is missing. The operative procedure has the following underlying principles: that for ear to sit naturally there must be an 8-10 mm wide rim of cartilage along the whole periphery; and the three layers of the ear, like layers of veneer, can be seaprated from each other and, as flaps, reconstruct a defect where the donor area of the ear only needs to have two layers. This reconstruction can be carried out ambulatorily, and gives satisfactory cosmetic and functional results.", "contents": "Laminate-plasty reconstruction of partial auricular defects A new method. The problems surrounding the reconstruction of partial defects of the external ear are examined, and a new method is presented for the reconstruction of defects of the helix where up to one-half is missing. The operative procedure has the following underlying principles: that for ear to sit naturally there must be an 8-10 mm wide rim of cartilage along the whole periphery; and the three layers of the ear, like layers of veneer, can be seaprated from each other and, as flaps, reconstruct a defect where the donor area of the ear only needs to have two layers. This reconstruction can be carried out ambulatorily, and gives satisfactory cosmetic and functional results."}
{"id": "PMID:1099646", "title": "Transfixation in cross-leg procedures using Hoffman's instruments. Report of forty-two cases.", "content": "The Hoffman transfixation equipment for fracture fixation gives simultaneously an excellent fixation of a flap in cross-leg procedures. Because of these favourable results in 42 cases this method for the fixation of the extremities in predicle flap procedures is recommended. It is possible to divide the flaps much earlier than is customary. In this material half of the flaps were divided on the 14th day or earlier. One or two preoperative flap delays are recommended. The method produces safer and faster healing of the flap.", "contents": "Transfixation in cross-leg procedures using Hoffman's instruments. Report of forty-two cases. The Hoffman transfixation equipment for fracture fixation gives simultaneously an excellent fixation of a flap in cross-leg procedures. Because of these favourable results in 42 cases this method for the fixation of the extremities in predicle flap procedures is recommended. It is possible to divide the flaps much earlier than is customary. In this material half of the flaps were divided on the 14th day or earlier. One or two preoperative flap delays are recommended. The method produces safer and faster healing of the flap."}
{"id": "PMID:1099647", "title": "[Diagnostic significance of the demonstration of fibrin and fibrinogen degradation products in the urine].", "content": "The concentration of fibrin/fibrinogen degradation products in urine (FDPu) was measured in samples obtained from 114 patients and 63 clinically healthy volunteers, once or repeatedly. The FDP titers measured by the hemagglutination inhibition (HI) method corresponded to less than 2.6 mug/ml FDP in all samples from healthy controls. Slightly elevated FDP concentrations were found in urine obtained from a few patients with disorders not primarily involving the urinary tract. The clinical importance of these isolated findings remains unclear. Urinary tract infections were not frequently accompanied by elevated FDPu concentrations. In patients with glomerulonephritis FDP excretion correlated somewhat better with severity of the renal affection. A further group of patients showed an unequivocal correlation between FDP excretion in the urine and postoperative complications following renal transplantation. However, the clinical diagnosis of acute rejection crisis was usually established at the same time or even before an increase in FDPu was found. Our results suggest that among diagnostic procedures the measurement of FDPu contributes little specific information for the evaluation of urinary tract disease. FDPu measurements in the immediate postoperative phase following renal transplantation may however be important for prognostic evaluation and, in individual cases, predict transplant rejection. We also attempted to define the FDPu qualitatively by simultaneous measurements using HI and the staphylococcal clumping test (SCT). Immunoelectrophoresis confirmed the well-known fact that the SCT detects only high-molecular FDP; this limits its clinical usefulness, despite its high sensitivity.", "contents": "[Diagnostic significance of the demonstration of fibrin and fibrinogen degradation products in the urine]. The concentration of fibrin/fibrinogen degradation products in urine (FDPu) was measured in samples obtained from 114 patients and 63 clinically healthy volunteers, once or repeatedly. The FDP titers measured by the hemagglutination inhibition (HI) method corresponded to less than 2.6 mug/ml FDP in all samples from healthy controls. Slightly elevated FDP concentrations were found in urine obtained from a few patients with disorders not primarily involving the urinary tract. The clinical importance of these isolated findings remains unclear. Urinary tract infections were not frequently accompanied by elevated FDPu concentrations. In patients with glomerulonephritis FDP excretion correlated somewhat better with severity of the renal affection. A further group of patients showed an unequivocal correlation between FDP excretion in the urine and postoperative complications following renal transplantation. However, the clinical diagnosis of acute rejection crisis was usually established at the same time or even before an increase in FDPu was found. Our results suggest that among diagnostic procedures the measurement of FDPu contributes little specific information for the evaluation of urinary tract disease. FDPu measurements in the immediate postoperative phase following renal transplantation may however be important for prognostic evaluation and, in individual cases, predict transplant rejection. We also attempted to define the FDPu qualitatively by simultaneous measurements using HI and the staphylococcal clumping test (SCT). Immunoelectrophoresis confirmed the well-known fact that the SCT detects only high-molecular FDP; this limits its clinical usefulness, despite its high sensitivity."}
{"id": "PMID:1099648", "title": "[Endocrine ophthalmology.  An overview of pathology, classification, and therapy].", "content": "A new uniform nomenclature will allow more accurate clinical study of endocrine ophthalmopathy, a disease that remains an enigma.  Clinical eye disease develops in 30-60% of patients with Graves' disease, but the severe malignant form occurs in a maximum of 5%, and most of these latter patients have been rendered euthyroid or hypothyroid by treatment of the hyperthyroidism.  Despite claims to the contrary, there is no convincing evidence that the mode of treatment (antithyroid drugs, surgery or radioiodine) has a bearing on the development of ophthalmopathy.  Circulating fragments of the TSH-molecule, which produce swelling of retroorbital tissue, and as autoimmune processes of the delayed type are currently under consideration as causative factors.  Medical treatment of endocrine ophthalmopathy has only a limited effect.  In cases of rapid progression with deterioration of vision it is recommended that operative decompression, preferably into the maxillary and ethmoidal sinus, be performed without delay.  Retroorbital administration of 2000 rad by a highly collimated megavoltage beam appears to be a valuable alternative to surgery.", "contents": "[Endocrine ophthalmology.  An overview of pathology, classification, and therapy]. A new uniform nomenclature will allow more accurate clinical study of endocrine ophthalmopathy, a disease that remains an enigma.  Clinical eye disease develops in 30-60% of patients with Graves' disease, but the severe malignant form occurs in a maximum of 5%, and most of these latter patients have been rendered euthyroid or hypothyroid by treatment of the hyperthyroidism.  Despite claims to the contrary, there is no convincing evidence that the mode of treatment (antithyroid drugs, surgery or radioiodine) has a bearing on the development of ophthalmopathy.  Circulating fragments of the TSH-molecule, which produce swelling of retroorbital tissue, and as autoimmune processes of the delayed type are currently under consideration as causative factors.  Medical treatment of endocrine ophthalmopathy has only a limited effect.  In cases of rapid progression with deterioration of vision it is recommended that operative decompression, preferably into the maxillary and ethmoidal sinus, be performed without delay.  Retroorbital administration of 2000 rad by a highly collimated megavoltage beam appears to be a valuable alternative to surgery."}
{"id": "PMID:1099652", "title": "Lymphoid response of the burn patient.", "content": "Clinical observations have long suggested immunologic compromise in burned patients. Resolution of the immune system into cooperative dual components of T-cells mediating delayed hypersensitivity and B-cells  mediating antibody responses prompted the present survey of T-cell and B-cell changes after acute thermal burn injury. Adults and children sustaining extensive burn injuries were studied for up to 60 days after injury. T-cell function was assessed by lymphocyte counts, in vitro lymphocyte synthesis of RNA and DNA with and without mitogenic stimulation, and lymphocyte response and stimulatory capacities in the mixed lymphocyte culture (MLC) reaction. B-cell function was studied by quantitation of the major classes of circulating immunoglobulins. Transient lymphocytopenia, increased RNA and DNA synthesis rates by both mitogenstimulated and unstimulated lymphocytes, and impairment of lymphocyte response and stimulation in the MLC were observed early in the postburn period. These parameters tended to normalize by the third week after injury. Immunoglobulin levels, particularly IgG, were depressed significantly the first week postburn, but were normal or elevated by 60 days. These studies document both T-cell and B-cell changes postburn,which appear to be reversible with the recovery phase. A transient shift of circulating lymphocytes toward the B-cell type is suggested and early depression of immunoglobulin levels is notable. Both types of changes likely contribute to the immunologic compromise of the acutely burned patient.", "contents": "Lymphoid response of the burn patient. Clinical observations have long suggested immunologic compromise in burned patients. Resolution of the immune system into cooperative dual components of T-cells mediating delayed hypersensitivity and B-cells  mediating antibody responses prompted the present survey of T-cell and B-cell changes after acute thermal burn injury. Adults and children sustaining extensive burn injuries were studied for up to 60 days after injury. T-cell function was assessed by lymphocyte counts, in vitro lymphocyte synthesis of RNA and DNA with and without mitogenic stimulation, and lymphocyte response and stimulatory capacities in the mixed lymphocyte culture (MLC) reaction. B-cell function was studied by quantitation of the major classes of circulating immunoglobulins. Transient lymphocytopenia, increased RNA and DNA synthesis rates by both mitogenstimulated and unstimulated lymphocytes, and impairment of lymphocyte response and stimulation in the MLC were observed early in the postburn period. These parameters tended to normalize by the third week after injury. Immunoglobulin levels, particularly IgG, were depressed significantly the first week postburn, but were normal or elevated by 60 days. These studies document both T-cell and B-cell changes postburn,which appear to be reversible with the recovery phase. A transient shift of circulating lymphocytes toward the B-cell type is suggested and early depression of immunoglobulin levels is notable. Both types of changes likely contribute to the immunologic compromise of the acutely burned patient."}
{"id": "PMID:1099659", "title": "Carcinoma of the renal pelvis: a ten-year review.", "content": "Twenty-two cases of renal pelvic carcinoma are reviewed, of which nine were squamous cell and 13 were transitional cell carcinomas. No difference in symptoms between the two types was seen except for an increased incidence of a palpable mass and weight loss among patients having the squamous cell variety. Thirteen of the 22 patients have died of renal pelvic carcinoma. Reasons for the low survival rate are discussed. Treatment of transitional cell carcinoma with nephroureterectomy and local lymph node dissection is recommended.", "contents": "Carcinoma of the renal pelvis: a ten-year review. Twenty-two cases of renal pelvic carcinoma are reviewed, of which nine were squamous cell and 13 were transitional cell carcinomas. No difference in symptoms between the two types was seen except for an increased incidence of a palpable mass and weight loss among patients having the squamous cell variety. Thirteen of the 22 patients have died of renal pelvic carcinoma. Reasons for the low survival rate are discussed. Treatment of transitional cell carcinoma with nephroureterectomy and local lymph node dissection is recommended."}
{"id": "PMID:1099663", "title": "Autologous bone marrow transplantation.", "content": "Bone marrow cells can be aspirated from patients with malignant disorders, stored at low temperatures, and reinfused into the same patient at a later date. In the past, the results of this autologous bone marrow transplantation were equivocal. With the availability of better methods for cryopreservation and more accurate assays for the survival of hemopoietic progenitor cells, there has been a resurgence of interest in this procedure.", "contents": "Autologous bone marrow transplantation. Bone marrow cells can be aspirated from patients with malignant disorders, stored at low temperatures, and reinfused into the same patient at a later date. In the past, the results of this autologous bone marrow transplantation were equivocal. With the availability of better methods for cryopreservation and more accurate assays for the survival of hemopoietic progenitor cells, there has been a resurgence of interest in this procedure."}
{"id": "PMID:1099664", "title": "Southern thoracic surgery.", "content": "The different cultures of North and South produced surgeons with subtle but identifiable differences in approaches to thoracic surgery. Some of these factors still represent a strength of the Southern thoracic surgeon, who is, at least spiritually, a descendant of early Southern surgical leaders. Background, contributions, observations, and attitudes of surgeons of the Confederate States of America are examined. Southern thoracic surgeons are urged to demand intellectual accomplishment and scientific achievement, even in the face of a changing, unstable, unpredictable sociopolitical environment of which we have little understanding and even less control.", "contents": "Southern thoracic surgery. The different cultures of North and South produced surgeons with subtle but identifiable differences in approaches to thoracic surgery. Some of these factors still represent a strength of the Southern thoracic surgeon, who is, at least spiritually, a descendant of early Southern surgical leaders. Background, contributions, observations, and attitudes of surgeons of the Confederate States of America are examined. Southern thoracic surgeons are urged to demand intellectual accomplishment and scientific achievement, even in the face of a changing, unstable, unpredictable sociopolitical environment of which we have little understanding and even less control."}
{"id": "PMID:1099691", "title": "The treatment of burns by early tangential excision and skin grafting.", "content": "The principles, aims and detailed techniques of tangential excision and skin grafting of burns are described. The results and advantages of the method in 28 burned patients are discussed.", "contents": "The treatment of burns by early tangential excision and skin grafting. The principles, aims and detailed techniques of tangential excision and skin grafting of burns are described. The results and advantages of the method in 28 burned patients are discussed."}
{"id": "PMID:1099692", "title": "Malaria prophylaxis.", "content": "A trial of malaria prophylaxis was carried out on 744 army personnel who were camping in an endemic area over a 3-month period. Three antimalarial drugs were used under strict control and the local population was used as a control, to prove that malaria was present during this time. No cases of malaria occurred in the trial group during this period.", "contents": "Malaria prophylaxis. A trial of malaria prophylaxis was carried out on 744 army personnel who were camping in an endemic area over a 3-month period. Three antimalarial drugs were used under strict control and the local population was used as a control, to prove that malaria was present during this time. No cases of malaria occurred in the trial group during this period."}
{"id": "PMID:1099694", "title": "Edendale Hospital, Pietermaritzburg. The first twenty-one years.", "content": "Edendale Hospital celebrates its twenty-first anniversary in 1975. Among non-teaching hospitals it has led the field in training and advancement of Black nurses and it has pioneered the employment of full-time specialists. It claims to have the first intensive care unit, the first central sterilising department, and the first postoperative recovery room in Southern Africa. Aspects of Edendale's early history are recorded.", "contents": "Edendale Hospital, Pietermaritzburg. The first twenty-one years. Edendale Hospital celebrates its twenty-first anniversary in 1975. Among non-teaching hospitals it has led the field in training and advancement of Black nurses and it has pioneered the employment of full-time specialists. It claims to have the first intensive care unit, the first central sterilising department, and the first postoperative recovery room in Southern Africa. Aspects of Edendale's early history are recorded."}
{"id": "PMID:1099697", "title": "Cell electrophoretic studies on the cellular immune response to Candida albicans in rabbits.", "content": "Rabbits were sensitized intracutaneously with Candida albicans total antigen by a series of booster injections. The electrophoretic migration time of lymph node lymphocytes was related to the time after injection. Two lymphocyte sub-populations could be distinguished. The proportion of each, changed during the course of the immune response. Characteristic changes also took place after in vitro incubation with Candidin. The results have been compared with agglutination titers and are discussed in relation to bursa and thymus-derived cells. The electrophoretic method may be valuable for the diagnosis of candidiasis.", "contents": "Cell electrophoretic studies on the cellular immune response to Candida albicans in rabbits. Rabbits were sensitized intracutaneously with Candida albicans total antigen by a series of booster injections. The electrophoretic migration time of lymph node lymphocytes was related to the time after injection. Two lymphocyte sub-populations could be distinguished. The proportion of each, changed during the course of the immune response. Characteristic changes also took place after in vitro incubation with Candidin. The results have been compared with agglutination titers and are discussed in relation to bursa and thymus-derived cells. The electrophoretic method may be valuable for the diagnosis of candidiasis."}
{"id": "PMID:1099698", "title": "Amino acid synthetic media for fungal pathogens based on aminopeptidase specificities: Histoplasma capsulatum, Blastomyces dermititidis, Paracoccidioides brasiliensis and Cryptococcus neoformans.", "content": "The development of simple and chemically defined liquid media for Histoplasma capsulatum, Blastomyces dermatitidis, Paracoccidioides brasiliensis and Crypto-occus neoformans according to their aminopeptidases profiles as amino acid requirement was described. When 1.5% purified agar was added, these media also supported excellent mycelial growth and sporulation of the deep mycoses. H. capsulatum was converted to and maintained in yeast phase when 0.1% L-cystine was added to the solid medium incubated at 37 degrees C.", "contents": "Amino acid synthetic media for fungal pathogens based on aminopeptidase specificities: Histoplasma capsulatum, Blastomyces dermititidis, Paracoccidioides brasiliensis and Cryptococcus neoformans. The development of simple and chemically defined liquid media for Histoplasma capsulatum, Blastomyces dermatitidis, Paracoccidioides brasiliensis and Crypto-occus neoformans according to their aminopeptidases profiles as amino acid requirement was described. When 1.5% purified agar was added, these media also supported excellent mycelial growth and sporulation of the deep mycoses. H. capsulatum was converted to and maintained in yeast phase when 0.1% L-cystine was added to the solid medium incubated at 37 degrees C."}
{"id": "PMID:1099699", "title": "Immunoglobulin classes of antibodies to Aspergillus fumigatus in patients with pulmonary aspergillosis.", "content": "An indirect immunofluorescence test was used to determine the titre and immunoglobulin classes of antibodies to Aspergillus fumigatus in human serum. In 15 patients with aspergillosis, significant titres of IgG but not IgA or IgM antibodies were found. The IgG, IgA and IgM fractions of serum from 2 patients with aspergillosis were separated. This confirmed that antibodies to A. fumigatus reside in highest titre in the IgG fraction. Precipitating antibodies to A. fumigatus in these patients also belonged to the IgG class of immunoglobulin.", "contents": "Immunoglobulin classes of antibodies to Aspergillus fumigatus in patients with pulmonary aspergillosis. An indirect immunofluorescence test was used to determine the titre and immunoglobulin classes of antibodies to Aspergillus fumigatus in human serum. In 15 patients with aspergillosis, significant titres of IgG but not IgA or IgM antibodies were found. The IgG, IgA and IgM fractions of serum from 2 patients with aspergillosis were separated. This confirmed that antibodies to A. fumigatus reside in highest titre in the IgG fraction. Precipitating antibodies to A. fumigatus in these patients also belonged to the IgG class of immunoglobulin."}
{"id": "PMID:1099700", "title": "Quantitative tissue invasion of the murine brain as a phenotypic marker of strain virulence in Aspergillus nidulans.", "content": "In this study the extent of fungal invasion in tissues of the target organs (brain, heart and kidney) has been quantitated histologically in DBA/2J mice inoculated intravenously with 3 strains of Aspergillus nidulans of different virulence. This was done to determine the relation between tissue invasion and strain virulence as determined by time-mortality bioassay. It was found that quantitatively tissue invasion by A. nidulans was target organ specific. Relative tissue invasion within each of the tissues examined was dependent on the strain of A. nidulans injected. In the brain a direct relationship between strain virulence and the extent of tissue invasion was found. This relationship was not observed in the other 2 target organs. The observations suggest that an important phenotypic marker of murine virulence in A. nidulans is the relative ability to invade the tissues of the murine brain.", "contents": "Quantitative tissue invasion of the murine brain as a phenotypic marker of strain virulence in Aspergillus nidulans. In this study the extent of fungal invasion in tissues of the target organs (brain, heart and kidney) has been quantitated histologically in DBA/2J mice inoculated intravenously with 3 strains of Aspergillus nidulans of different virulence. This was done to determine the relation between tissue invasion and strain virulence as determined by time-mortality bioassay. It was found that quantitatively tissue invasion by A. nidulans was target organ specific. Relative tissue invasion within each of the tissues examined was dependent on the strain of A. nidulans injected. In the brain a direct relationship between strain virulence and the extent of tissue invasion was found. This relationship was not observed in the other 2 target organs. The observations suggest that an important phenotypic marker of murine virulence in A. nidulans is the relative ability to invade the tissues of the murine brain."}
{"id": "PMID:1099702", "title": "Respiratory care following open heart surgery.", "content": "Respiratory care of patients undergoing open heart surgery should begin in the preoperative period. Patients must stop smoking, and if obese they are encouraged to lose weight. Pulmonary infection is treated and secretions must be eliminated. Postoperative hypoxemia, which is an expected event following anesthesia and surgery, is aggravated by circulatory instability and pulmonary complications. Following open heart surgery pulmonary complications such as atelectasis, congestion, edema, postperfusion lung, pneumothorax, pleural effusion, and hemothorax are common. Respiratory care should be planned to avoid these complications and to treat them promptly should they occur. Routinely every patient is mechanically ventilated for at least 12 to 18 hours following surgery. The type of ventilator used and its parameters are adjusted according to the clinical condition of the patient to maintain adequate oxygenation and to prevent any respiratory acidosis. When indicated, PEEP is applied to improve arterial oxygenation. Respiratory care is extended for at least 5 days after termination of artificial ventilation. Oxygen therapy is given with either a nasal catheter or a mask, according to the patient's need. IPPB and physiotherapy are continued until the patient shows no signs of pulmonary infection and is capable of effectively eliminating secretions. This routine management and extended postoperative respiratory care definitely contribute to the successful outcome of open heart surgery.", "contents": "Respiratory care following open heart surgery. Respiratory care of patients undergoing open heart surgery should begin in the preoperative period. Patients must stop smoking, and if obese they are encouraged to lose weight. Pulmonary infection is treated and secretions must be eliminated. Postoperative hypoxemia, which is an expected event following anesthesia and surgery, is aggravated by circulatory instability and pulmonary complications. Following open heart surgery pulmonary complications such as atelectasis, congestion, edema, postperfusion lung, pneumothorax, pleural effusion, and hemothorax are common. Respiratory care should be planned to avoid these complications and to treat them promptly should they occur. Routinely every patient is mechanically ventilated for at least 12 to 18 hours following surgery. The type of ventilator used and its parameters are adjusted according to the clinical condition of the patient to maintain adequate oxygenation and to prevent any respiratory acidosis. When indicated, PEEP is applied to improve arterial oxygenation. Respiratory care is extended for at least 5 days after termination of artificial ventilation. Oxygen therapy is given with either a nasal catheter or a mask, according to the patient's need. IPPB and physiotherapy are continued until the patient shows no signs of pulmonary infection and is capable of effectively eliminating secretions. This routine management and extended postoperative respiratory care definitely contribute to the successful outcome of open heart surgery."}
{"id": "PMID:1099703", "title": "Use of ileal reservoir following proctocolectomy.", "content": "A study of 37 patients who underwent ileostomy and creation of an ileal reservoir revealed that all had satisfactory pouches. In the 24 patients in whom a nipple valve was created, the stoma was continent in 21. In the patients without a nipple valve, about one-half are continent. There have been no skin problems in any of the 37 patients. In two patients, the reservoir has been removed, one because of obstruction and the other at the election of the patinet. Although ileostomy with an ileal reservoir does not solve all of the problems, it offers patients who have undergone proctocolectomy for chonic ulcerative colitis or multiple neoplastic disease a better quality of life than does ileostomy alone. An appliance need not be worn constantly, and the stoma can be flush with the skin edge and placed low in the abdominal wall. Nevertheless, management problems do exist in some patients, and the patients are exposed to complications similar to those in any patient who has undergone a major abdominal surgical procedure that involves resection of intestine and creation of an intestinal stoma. Our group is optimistic regarding the use of this procedure in selected patients.", "contents": "Use of ileal reservoir following proctocolectomy. A study of 37 patients who underwent ileostomy and creation of an ileal reservoir revealed that all had satisfactory pouches. In the 24 patients in whom a nipple valve was created, the stoma was continent in 21. In the patients without a nipple valve, about one-half are continent. There have been no skin problems in any of the 37 patients. In two patients, the reservoir has been removed, one because of obstruction and the other at the election of the patinet. Although ileostomy with an ileal reservoir does not solve all of the problems, it offers patients who have undergone proctocolectomy for chonic ulcerative colitis or multiple neoplastic disease a better quality of life than does ileostomy alone. An appliance need not be worn constantly, and the stoma can be flush with the skin edge and placed low in the abdominal wall. Nevertheless, management problems do exist in some patients, and the patients are exposed to complications similar to those in any patient who has undergone a major abdominal surgical procedure that involves resection of intestine and creation of an intestinal stoma. Our group is optimistic regarding the use of this procedure in selected patients."}
{"id": "PMID:1099719", "title": "[Dopamine effects on the pulmonary circulation of innervated and autotransplanted lungs (author's transl)].", "content": "The effects of dopamine on the pulmonary vascular resistance were investigated in 7 normal and 9 autotransplanted canine lungs during perfusion in situ. Dopamine (up to 13,5 gamma/kg BW, rapid injection) acts as a vasoconstrictor by alpha-receptor stimulation. Vasodilative effects could not be registered neither by very small amounts of dopamine nor during alpha-blockade with phentolamine. No difference could be detected between the vascular reactions of normal and autotransplanted lungs.", "contents": "[Dopamine effects on the pulmonary circulation of innervated and autotransplanted lungs (author's transl)]. The effects of dopamine on the pulmonary vascular resistance were investigated in 7 normal and 9 autotransplanted canine lungs during perfusion in situ. Dopamine (up to 13,5 gamma/kg BW, rapid injection) acts as a vasoconstrictor by alpha-receptor stimulation. Vasodilative effects could not be registered neither by very small amounts of dopamine nor during alpha-blockade with phentolamine. No difference could be detected between the vascular reactions of normal and autotransplanted lungs."}
{"id": "PMID:1099725", "title": "[Adhesive sealing of fissures and pits for the prevention of caries. Review].", "content": "Conformably to the order received, the present contribution aims, as desired by the editor, at meeting the information requirements of the practitioner as completely as possible. For that reason, the cruical points are stressed. This complex of questions is also analysed from the aspect of the author's opinion. This analysis is intended for aiding the reader in processing source material and in forming his own opinion. Starting from the epidemiological foundation of the necessity of caries prevention, the author integrates the method of adhesive sealing and describes the necessary materials and the various steps of the procedure. The article centers in a critical interpretation of its preventive efficiency in clinic and experiment and ends in an outlook.", "contents": "[Adhesive sealing of fissures and pits for the prevention of caries. Review]. Conformably to the order received, the present contribution aims, as desired by the editor, at meeting the information requirements of the practitioner as completely as possible. For that reason, the cruical points are stressed. This complex of questions is also analysed from the aspect of the author's opinion. This analysis is intended for aiding the reader in processing source material and in forming his own opinion. Starting from the epidemiological foundation of the necessity of caries prevention, the author integrates the method of adhesive sealing and describes the necessary materials and the various steps of the procedure. The article centers in a critical interpretation of its preventive efficiency in clinic and experiment and ends in an outlook."}
{"id": "PMID:1099726", "title": "[Prosthetic aids in the resection of the middle portion of the mandible].", "content": "Prosthetic aids in resections of the middle piece of the mandible. The authors describe a prosthesis which is inserted during the resection of the middle piece of the mandible. Due to its construction it prevents the soft tissues of the floor of the mouth from sinking back. Such a prosthesis has been used in 18 cases. Respiratory complications did not occur; no tracheotomy was necessary. During the postoperative phase, the prosthesis came up to expectations, but it could not prevent cicatricial contraction in the operating area. Occlusion-conformable positioning of the fragments will be achieved only it the remaining teeth are suited for function-stable prosthetic care. Consequently, secondary osteoplasty should not be abandoned.", "contents": "[Prosthetic aids in the resection of the middle portion of the mandible]. Prosthetic aids in resections of the middle piece of the mandible. The authors describe a prosthesis which is inserted during the resection of the middle piece of the mandible. Due to its construction it prevents the soft tissues of the floor of the mouth from sinking back. Such a prosthesis has been used in 18 cases. Respiratory complications did not occur; no tracheotomy was necessary. During the postoperative phase, the prosthesis came up to expectations, but it could not prevent cicatricial contraction in the operating area. Occlusion-conformable positioning of the fragments will be achieved only it the remaining teeth are suited for function-stable prosthetic care. Consequently, secondary osteoplasty should not be abandoned."}
{"id": "PMID:1099727", "title": "Specificity and immunosuppressive potency of a rabbit antimouse T cell-specific antiserum.", "content": "An attempt was made to prepare a specific heterologous rabbit antimouse T cell antiserum (anti-MTLA) by absorbing rabbit antimouse thymocyte globulin (ATG) with spleen cells from BALB/c TXBM mice. Cytotoxicity data showed that whereas ATG was cytotoxic to both T and B cells, anti-MTLA was highly cytotoxic to only T cells. Whereas spleen cells treated with ATG and complement (C) failed to respond in all assays studied, spleen cells treated with anti-MTLA and C: (1) responded to the B cell mitogens but failed to respond to the T cell mitogens; (2) were able to stimulate allogeneic spleen cells but failed to respond to mixed lymphocyte culture (3) failed to act as T killer cells in the CML reaction but retained their ability to kill antibody-coated target cells; and (4) did not cause a graft-versus-host reaction when injected in allogeneic mice and increased their survival significantly. Furthermore, anti-MTLA was just as immunosuppressive in vivo as ATG in its ability to suppress the immune response to sheep red blood cells and prolong skin allograft survival. Anti-MTLA was found to be different in specificities from anti-theta serum by several points: (1) it was cytotoxic for T cells from both theta-C3H and theta-AKR mice; (2) it was highly immunosuppressive in vivo when compared to anti-theta serum; (3) absorption of anti-MTLA with mouse brain did not decrease the immunosuppressive activity; and (4) rabbit antimouse brain antiserum failed to show any immunosuppressive activity. These data indicate that anti-MTLA is a specific antiserum against a unique marker on T cells distinct from the theta marker.", "contents": "Specificity and immunosuppressive potency of a rabbit antimouse T cell-specific antiserum. An attempt was made to prepare a specific heterologous rabbit antimouse T cell antiserum (anti-MTLA) by absorbing rabbit antimouse thymocyte globulin (ATG) with spleen cells from BALB/c TXBM mice. Cytotoxicity data showed that whereas ATG was cytotoxic to both T and B cells, anti-MTLA was highly cytotoxic to only T cells. Whereas spleen cells treated with ATG and complement (C) failed to respond in all assays studied, spleen cells treated with anti-MTLA and C: (1) responded to the B cell mitogens but failed to respond to the T cell mitogens; (2) were able to stimulate allogeneic spleen cells but failed to respond to mixed lymphocyte culture (3) failed to act as T killer cells in the CML reaction but retained their ability to kill antibody-coated target cells; and (4) did not cause a graft-versus-host reaction when injected in allogeneic mice and increased their survival significantly. Furthermore, anti-MTLA was just as immunosuppressive in vivo as ATG in its ability to suppress the immune response to sheep red blood cells and prolong skin allograft survival. Anti-MTLA was found to be different in specificities from anti-theta serum by several points: (1) it was cytotoxic for T cells from both theta-C3H and theta-AKR mice; (2) it was highly immunosuppressive in vivo when compared to anti-theta serum; (3) absorption of anti-MTLA with mouse brain did not decrease the immunosuppressive activity; and (4) rabbit antimouse brain antiserum failed to show any immunosuppressive activity. These data indicate that anti-MTLA is a specific antiserum against a unique marker on T cells distinct from the theta marker."}
{"id": "PMID:1099728", "title": "The role of the spleen in the rejection and enhancement of renal allografts in the rat.", "content": "As rats were splenectomised up to 7 weeks before or at the time of receiving an (AS times August)F1 kidney allograft. Only 2 of 19 splenectomised recipients rejected their grafts within 12 days, and 14 of the 19 survived for more than 100 days. All 19 splenectomised recipients had a marked and often complete suppression of the lymphocytotoxic antibody response to the graft. In contrast, splenecotomy had no effect in suppressing the rejection of (AS times August)F1 renal allografts transplanted to AS recipients previously immunised with August lymphoid tissue, or in AS recipients of homozygous August renal allografts. Splenectomised recipients could be reconstituted with spleen cells from sensitised but not from normal AS rats. Splenectomy was found neither to augment nor diminish the effectiveness of passive enhancement of (AS times August)F1 or August kidneys in AS recipients. Splenectomy of AS recipients 2 or 4 days after an (AS times August)F1 renal allograft was as effective as pregraft splenectomy in suppressing rejection, but there was a deficiency of long survivors in a group splenectomised 1 day after grafting. A working hypothesis to explain these results is presented, and their clinical relevance is discussed.", "contents": "The role of the spleen in the rejection and enhancement of renal allografts in the rat. As rats were splenectomised up to 7 weeks before or at the time of receiving an (AS times August)F1 kidney allograft. Only 2 of 19 splenectomised recipients rejected their grafts within 12 days, and 14 of the 19 survived for more than 100 days. All 19 splenectomised recipients had a marked and often complete suppression of the lymphocytotoxic antibody response to the graft. In contrast, splenecotomy had no effect in suppressing the rejection of (AS times August)F1 renal allografts transplanted to AS recipients previously immunised with August lymphoid tissue, or in AS recipients of homozygous August renal allografts. Splenectomised recipients could be reconstituted with spleen cells from sensitised but not from normal AS rats. Splenectomy was found neither to augment nor diminish the effectiveness of passive enhancement of (AS times August)F1 or August kidneys in AS recipients. Splenectomy of AS recipients 2 or 4 days after an (AS times August)F1 renal allograft was as effective as pregraft splenectomy in suppressing rejection, but there was a deficiency of long survivors in a group splenectomised 1 day after grafting. A working hypothesis to explain these results is presented, and their clinical relevance is discussed."}
{"id": "PMID:1099729", "title": "The treatment of rejection. A trial of acetylsalicylic acid, dipyridamole, and heparin.", "content": "Serial studies of platelet and fibrinogen survival were performed in 26 nonimmunosuppressed dogs after allogenic renal transplant operations. Treatment with acetylsalicylic acid, dipyridamole, and heparin failed to improve the selective platelet destruction which occurred in untreated animals, and it did not improve postoperative longevity. There was a high incidence of postoperative wound and intrarenal hemorrhage after heparin treatment. These results are consistent with the hypothesis that platelet destruction is a consequence rather than the cause of acute graft rejection, and it is concluded that antithrombotic therapy is not of practical benefit in preventing acute rejection.", "contents": "The treatment of rejection. A trial of acetylsalicylic acid, dipyridamole, and heparin. Serial studies of platelet and fibrinogen survival were performed in 26 nonimmunosuppressed dogs after allogenic renal transplant operations. Treatment with acetylsalicylic acid, dipyridamole, and heparin failed to improve the selective platelet destruction which occurred in untreated animals, and it did not improve postoperative longevity. There was a high incidence of postoperative wound and intrarenal hemorrhage after heparin treatment. These results are consistent with the hypothesis that platelet destruction is a consequence rather than the cause of acute graft rejection, and it is concluded that antithrombotic therapy is not of practical benefit in preventing acute rejection."}
{"id": "PMID:1099730", "title": "Enhancement of skin grafts in the mouse. The combined used of specific and nonspecific immunosuppression.", "content": "Enhancement of skin grafts in mice by passively administered alloantiserum was examined in conjunction with the simultaneous use of other immunosuppressive regimens. It could be clearly shown that when a \"weak\" antilymphocyte serum (ALS) was used, a significant further prolongation of graft survival occurred over that obtained with the enhancing antiserum alone, and these two separate effects were synergistic. When a \"strong\" ALS was used, no synergistic effects were apparent unless the enhancing alloantiserum was given almost continuously. A similar, but less impressive synergism was seen when the enhancing alloantiserum and azathioprine were used together. Azathioprine, like ALS, presumably acts on T cells, whereas drugs directed against B cells, such as cyclophosphamide and prednisolone, failed to show any synergistic effect.", "contents": "Enhancement of skin grafts in the mouse. The combined used of specific and nonspecific immunosuppression. Enhancement of skin grafts in mice by passively administered alloantiserum was examined in conjunction with the simultaneous use of other immunosuppressive regimens. It could be clearly shown that when a \"weak\" antilymphocyte serum (ALS) was used, a significant further prolongation of graft survival occurred over that obtained with the enhancing antiserum alone, and these two separate effects were synergistic. When a \"strong\" ALS was used, no synergistic effects were apparent unless the enhancing alloantiserum was given almost continuously. A similar, but less impressive synergism was seen when the enhancing alloantiserum and azathioprine were used together. Azathioprine, like ALS, presumably acts on T cells, whereas drugs directed against B cells, such as cyclophosphamide and prednisolone, failed to show any synergistic effect."}
{"id": "PMID:1099734", "title": "Kidney preservation using combinations of Belzer and Collins techniques.", "content": "A study was designed to test the advisability of using combined Belzer and Collins preservation in kidney-sharing programs. Dogs were randomly assigned to seven groups and no donor pretreatment was used. In group 1, left kidneys were removed from dogs; were flushed with Collins solution; and were autotransplanted immediately. In groups 2 through 7, dog kidneys were preserved for 24 hr using combined techniques. The percentage of Collins preservation included in each group was increased progressively until, in Group 7, Collins alone was used. Contralateral nephrectomies were performed at the time of autotransplantation. Between 4 and 8 dogs survived in each group, yielding 43 6-week survivors. Sixty-six per cent of group 7 animals and greater than 90% of animals in other groups survived 6 weeks after transplantation. Average creatinine and blood urea nitrogen values on days 3,7, and 42 showed a progressive increase in kidney damage from groups 1 through 7. As the percentage of time the kidneys were subjected to Collins preservation was increased, the quality of kidney preservation decreased in a progressive manner. This study suggests that Belzer and Collins techniques can be combined, but superior preservation was obtained when the majority of preservation was on the Belzer apparatus.", "contents": "Kidney preservation using combinations of Belzer and Collins techniques. A study was designed to test the advisability of using combined Belzer and Collins preservation in kidney-sharing programs. Dogs were randomly assigned to seven groups and no donor pretreatment was used. In group 1, left kidneys were removed from dogs; were flushed with Collins solution; and were autotransplanted immediately. In groups 2 through 7, dog kidneys were preserved for 24 hr using combined techniques. The percentage of Collins preservation included in each group was increased progressively until, in Group 7, Collins alone was used. Contralateral nephrectomies were performed at the time of autotransplantation. Between 4 and 8 dogs survived in each group, yielding 43 6-week survivors. Sixty-six per cent of group 7 animals and greater than 90% of animals in other groups survived 6 weeks after transplantation. Average creatinine and blood urea nitrogen values on days 3,7, and 42 showed a progressive increase in kidney damage from groups 1 through 7. As the percentage of time the kidneys were subjected to Collins preservation was increased, the quality of kidney preservation decreased in a progressive manner. This study suggests that Belzer and Collins techniques can be combined, but superior preservation was obtained when the majority of preservation was on the Belzer apparatus."}
{"id": "PMID:1099735", "title": "Characteristics of specific unresponsiveness toward kidney and skin allografts in adult rats inoculated at birth with allogenic bone marrow or kidney cells across a strong H-1 barrier.", "content": "Specific unresponsiveness inducible in newborn Lewis (Le) rats by injection of bone marrow or kidney cells from (Bu x Le)F1 hybrids was tested in adults by allografting of skin or kidneys from strongly incompatible Buffalo (Bu) strain donors. High doses of bone marrow cells (10(7) or more) proved quite effective in procuring indefinite survival of subsequent Bu kidney allografts, but not Bu skin allografts. Kidney allografts remained fully functional without modulation of their inherent immunogenicity regardless whether skin allografts were acutely rejected before or after kidney grafting on bone marrow-tolerant recipients. Lower doses of bone marrow cells were either near threshold for induction of specific Bu kidney allograft tolerance (5 x 10(6)) or, conversely, led to increased immunity(1-5 x 106). A strikingly different pattern of reactivity was found in adult Le recipients of Bu skin allografts as shown by only a moderate, but stepwise increase in survival times as a function of increasing allogeneic cell dosage at birth. Allogeneic kidney cells at all doses were surprisingly ineffective in inducing tolerance of either kidney or skin allografts, so the existence of kidney-specific transplantation antigens remains problematical. Lymphocytes from kidney allograft-tolerant recipients showed much reduced but still significant antidonor activity in several tests of cell-mediated immunity in vitro and in vivo. Blocking antibody activity was also demonstrable in these animals. Acquired tolerance (i.e., essential nonreactivity) may not only exist in degrees in either T or B cell pathways but may coexist with specific immunoblocking reactions in a dynamic equilibrium. Instead of tolerance versus enhancement, a new concept of selective specific immunoregulation emerges.", "contents": "Characteristics of specific unresponsiveness toward kidney and skin allografts in adult rats inoculated at birth with allogenic bone marrow or kidney cells across a strong H-1 barrier. Specific unresponsiveness inducible in newborn Lewis (Le) rats by injection of bone marrow or kidney cells from (Bu x Le)F1 hybrids was tested in adults by allografting of skin or kidneys from strongly incompatible Buffalo (Bu) strain donors. High doses of bone marrow cells (10(7) or more) proved quite effective in procuring indefinite survival of subsequent Bu kidney allografts, but not Bu skin allografts. Kidney allografts remained fully functional without modulation of their inherent immunogenicity regardless whether skin allografts were acutely rejected before or after kidney grafting on bone marrow-tolerant recipients. Lower doses of bone marrow cells were either near threshold for induction of specific Bu kidney allograft tolerance (5 x 10(6)) or, conversely, led to increased immunity(1-5 x 106). A strikingly different pattern of reactivity was found in adult Le recipients of Bu skin allografts as shown by only a moderate, but stepwise increase in survival times as a function of increasing allogeneic cell dosage at birth. Allogeneic kidney cells at all doses were surprisingly ineffective in inducing tolerance of either kidney or skin allografts, so the existence of kidney-specific transplantation antigens remains problematical. Lymphocytes from kidney allograft-tolerant recipients showed much reduced but still significant antidonor activity in several tests of cell-mediated immunity in vitro and in vivo. Blocking antibody activity was also demonstrable in these animals. Acquired tolerance (i.e., essential nonreactivity) may not only exist in degrees in either T or B cell pathways but may coexist with specific immunoblocking reactions in a dynamic equilibrium. Instead of tolerance versus enhancement, a new concept of selective specific immunoregulation emerges."}
{"id": "PMID:1099736", "title": "Hyperacute rejection of skin allografts in the mouse. Sensitivity of ingrowing skin grafts to the action of alloantibody and rabbit complement.", "content": "The destructive action of alloantiserum and exogenous complement on ingrowing skin allografts was studied. B6AF1 recipients of a B10.D2 skin graft received a single intravenous injection of B6AF1 anti-B10.D2 serum (antiserum to H-2K.31) together with rabbit complement (RC) within the first 10 days after transplantation. Different models were used: recipients without immunosuppression, recipients treated with antilymphocyte serum, x-irradiation, or enhancing antibody. If the injection was given between day 5 and 10 after grafting, hyperacute rejection occurred in all cases. The rejection seemed to be most violent when the injection was given on days 7 or 8. Injections given on days 1, 2 or 3 after grafting could not induce hyperacute-rejection, but resulted, on the contrary, in a prolongation of graft survival, probably due to immunological enhancement. Injections on day 4 produced patchy necrosis, but the grafts recovered and the residual tissue showed a prolonged survival. The results suggest that the presence of a functioning vascular network is a prerequisite for the occurrence of hyperacute rejection of skin allografts in the mouse.", "contents": "Hyperacute rejection of skin allografts in the mouse. Sensitivity of ingrowing skin grafts to the action of alloantibody and rabbit complement. The destructive action of alloantiserum and exogenous complement on ingrowing skin allografts was studied. B6AF1 recipients of a B10.D2 skin graft received a single intravenous injection of B6AF1 anti-B10.D2 serum (antiserum to H-2K.31) together with rabbit complement (RC) within the first 10 days after transplantation. Different models were used: recipients without immunosuppression, recipients treated with antilymphocyte serum, x-irradiation, or enhancing antibody. If the injection was given between day 5 and 10 after grafting, hyperacute rejection occurred in all cases. The rejection seemed to be most violent when the injection was given on days 7 or 8. Injections given on days 1, 2 or 3 after grafting could not induce hyperacute-rejection, but resulted, on the contrary, in a prolongation of graft survival, probably due to immunological enhancement. Injections on day 4 produced patchy necrosis, but the grafts recovered and the residual tissue showed a prolonged survival. The results suggest that the presence of a functioning vascular network is a prerequisite for the occurrence of hyperacute rejection of skin allografts in the mouse."}
{"id": "PMID:1099737", "title": "Surface properties and functional characteristics of infiltrating cells harvested from acutely rejecting cardiac allografts in inbred rats.", "content": "Viable, functioning effector cells exerting donor specific cytotoxic properties have been removed, with their surface properties intact, from acutely rejecting cardiac allografts in rats to define immunological events occurring within the grafts themselves. Macrophages comprised 15 to 25% of the cells harvested; lymphocytes comprised about 75%. A few polymorphonuclear leukocytes (approximately 5%) were present consistently. Lymphocytes bearing surface Ig, B cells, made up 35 to 47% of the mononuclear cells collected. The remaining lymphocytes were presumptive T cells. The surfact attributes of the infiltrating cells were compared to those of cells from recipient peripheral blood, spleen and lymph nodes, and to those from isografted controls. Many of the B lymphocytes possessed Fc receptors but were virtually unable to form erythrocyteantibody complement (EAC) rosettes, in contrast to splenocytes which formed approximately 30% EAC rosettes. Specific cytotoxicity against donor alloantigen-bearing cells, as tested in a 51Cr release assay, was shown to be a T cell function by serial fractionation experiments. Peak cytotoxicity of infiltrating T cells occurred early during the rejection process, while that of cells from recipient blood and lymphoid tissues occurred only following complete graft destruction. Antibody-dependent lymphocyte-mediated cytotoxicity (Ab-LMC) was demonstrated following gentle trypsinization or overnight incubation of the cells. Differing effector cell populations could be distinguished by treatment of anti-Ig and complement; direct donor-specific lymphocyte-mediated cytotoxicity was not disrupted by this treatment, while Ab-LMC was abolished.", "contents": "Surface properties and functional characteristics of infiltrating cells harvested from acutely rejecting cardiac allografts in inbred rats. Viable, functioning effector cells exerting donor specific cytotoxic properties have been removed, with their surface properties intact, from acutely rejecting cardiac allografts in rats to define immunological events occurring within the grafts themselves. Macrophages comprised 15 to 25% of the cells harvested; lymphocytes comprised about 75%. A few polymorphonuclear leukocytes (approximately 5%) were present consistently. Lymphocytes bearing surface Ig, B cells, made up 35 to 47% of the mononuclear cells collected. The remaining lymphocytes were presumptive T cells. The surfact attributes of the infiltrating cells were compared to those of cells from recipient peripheral blood, spleen and lymph nodes, and to those from isografted controls. Many of the B lymphocytes possessed Fc receptors but were virtually unable to form erythrocyteantibody complement (EAC) rosettes, in contrast to splenocytes which formed approximately 30% EAC rosettes. Specific cytotoxicity against donor alloantigen-bearing cells, as tested in a 51Cr release assay, was shown to be a T cell function by serial fractionation experiments. Peak cytotoxicity of infiltrating T cells occurred early during the rejection process, while that of cells from recipient blood and lymphoid tissues occurred only following complete graft destruction. Antibody-dependent lymphocyte-mediated cytotoxicity (Ab-LMC) was demonstrated following gentle trypsinization or overnight incubation of the cells. Differing effector cell populations could be distinguished by treatment of anti-Ig and complement; direct donor-specific lymphocyte-mediated cytotoxicity was not disrupted by this treatment, while Ab-LMC was abolished."}
{"id": "PMID:1099744", "title": "Effect of anti-donor serum (ADS) and donor cells on renal allograft survival in DL-A tissue-typed littermate beagles.", "content": "The influence of passive and active immunization on kidney allograft survival was tested in littermate beagles differing in one and two DL-A haplotypes. Immunization by treatment of recipients with hyperimmune antidonor serum (ADS) prolonged graft survival only in a few donor-recipient combinations differing in one DL-A haplotype. All ADS batches that were tested in MLC reaction did block this reaction, but no correlation with its in vivo activities was found. Hyperimmunization of recipients with donor lymphoid cells before grafting caused a second-set rejection, whereas one injection with donor blood did so only in 14%, with antibody production and accelerated rejection. A short course of horse antidog lymphocyte serum (HADLS) prolonged kidney graft survival. The addition of intravenously given donor bone-marrow cells to this protocol did not lead to a further increase in graft survival.", "contents": "Effect of anti-donor serum (ADS) and donor cells on renal allograft survival in DL-A tissue-typed littermate beagles. The influence of passive and active immunization on kidney allograft survival was tested in littermate beagles differing in one and two DL-A haplotypes. Immunization by treatment of recipients with hyperimmune antidonor serum (ADS) prolonged graft survival only in a few donor-recipient combinations differing in one DL-A haplotype. All ADS batches that were tested in MLC reaction did block this reaction, but no correlation with its in vivo activities was found. Hyperimmunization of recipients with donor lymphoid cells before grafting caused a second-set rejection, whereas one injection with donor blood did so only in 14%, with antibody production and accelerated rejection. A short course of horse antidog lymphocyte serum (HADLS) prolonged kidney graft survival. The addition of intravenously given donor bone-marrow cells to this protocol did not lead to a further increase in graft survival."}
{"id": "PMID:1099747", "title": "Comparison of tritiated hypoxanthine, adenine and adenosine for purine-salvage incorporation into nucleic acids of the malarial parasite, Plasmodium berghei.", "content": "This study was accomplished to examine the relative importance of different metabolic precursors of nucleic acid synthesis in the malarial parasite, P. berghei. Three possible pathways for incorporation of Adenine (type) compounds exist: 1) incorporation via hypoxanthine, 2) via adenine, or 3) via adenosine. The parasitized cell and erythrocyte-free malarial parasite were both examined because of possible metabolic differences that could be encountered. Hypoxanthine was clearly the best precursor at both levels with extra-incorporation in the presence of allopurinol (10(-4)M), which protects oxidative metabolism of hypoxanthine. Adenosine was less efficient in its incorporation into nucleic acids at both levels. Adenine was clearly the poorest precursor being extremely less efficient compared to hypoxanthine 1/50 at parasitized cell level and 1/100 at the free parasite level. At both levels adenine seemed to be slightly more efficient in the presence of allopurinol and this appeared to be a similar to the incorporation via adenosine with allopurinol. In both cases, part of the incorporation could be coming via conversion to hypoxanthine because allopurinol protects oxidation of hypoxanthine via inhibition of xanthine oxidase. With the prior observation of Manandhar and Van Dyke that adenosine is converted to hypoxanthine outside or on the surface of the malarial parasite one is lead to conclude that of the three pathways the hypoxanthine pathway is probably the major and possibly the almost totally important pathway making hypoxanthine's uptake and/or conversion to inosine monophosphate a key event of metabolic and chemotherapeutic importance.", "contents": "Comparison of tritiated hypoxanthine, adenine and adenosine for purine-salvage incorporation into nucleic acids of the malarial parasite, Plasmodium berghei. This study was accomplished to examine the relative importance of different metabolic precursors of nucleic acid synthesis in the malarial parasite, P. berghei. Three possible pathways for incorporation of Adenine (type) compounds exist: 1) incorporation via hypoxanthine, 2) via adenine, or 3) via adenosine. The parasitized cell and erythrocyte-free malarial parasite were both examined because of possible metabolic differences that could be encountered. Hypoxanthine was clearly the best precursor at both levels with extra-incorporation in the presence of allopurinol (10(-4)M), which protects oxidative metabolism of hypoxanthine. Adenosine was less efficient in its incorporation into nucleic acids at both levels. Adenine was clearly the poorest precursor being extremely less efficient compared to hypoxanthine 1/50 at parasitized cell level and 1/100 at the free parasite level. At both levels adenine seemed to be slightly more efficient in the presence of allopurinol and this appeared to be a similar to the incorporation via adenosine with allopurinol. In both cases, part of the incorporation could be coming via conversion to hypoxanthine because allopurinol protects oxidation of hypoxanthine via inhibition of xanthine oxidase. With the prior observation of Manandhar and Van Dyke that adenosine is converted to hypoxanthine outside or on the surface of the malarial parasite one is lead to conclude that of the three pathways the hypoxanthine pathway is probably the major and possibly the almost totally important pathway making hypoxanthine's uptake and/or conversion to inosine monophosphate a key event of metabolic and chemotherapeutic importance."}
{"id": "PMID:1099748", "title": "[Comparative morphological studies on the kinetoplast of various species of trypanosomes, with special reference to trypanosoma cruzi (author's transl)].", "content": "Comparative electron microscope studies on the morphology of the kinetoplast DNA (K-DNA) of the epimastigotes in many trypanosome species were carried out under standardized conditions. The K-DNA shows a morphological variation during the cell cycle of culture forms of the trypanosome species under study. In longitudinal sections of the kinetoplast, the K-DNA of T. cruzi appears as a compact trabecular structure; as a relatively disaggregated unit, with a central band or in a transitional stage between these forms. The conspicuous central band of the K-DNA which occurs at the beginning of cell division when the basal body is duplicated, could be demonstrated in all the 8 T. cruzi isolates studied. This has been found in the human-pathogen strains as well as in the T. cruzi-like trypanosomes of wild animals. In contrast, in comparable developmental stages of T. conorhini, T. rangeli and two strains of T. lewisi, this structural configuration of the K-DNA could not be observed. Based on these results, the extent to which the central band of K-DNA may be used in differentiating between trypanosomes is discussed. These findings may also reflect the present state of knowledge, as based on the study of 12 trypanosome isolates, so that corrections or additions may be lateron possible.", "contents": "[Comparative morphological studies on the kinetoplast of various species of trypanosomes, with special reference to trypanosoma cruzi (author's transl)]. Comparative electron microscope studies on the morphology of the kinetoplast DNA (K-DNA) of the epimastigotes in many trypanosome species were carried out under standardized conditions. The K-DNA shows a morphological variation during the cell cycle of culture forms of the trypanosome species under study. In longitudinal sections of the kinetoplast, the K-DNA of T. cruzi appears as a compact trabecular structure; as a relatively disaggregated unit, with a central band or in a transitional stage between these forms. The conspicuous central band of the K-DNA which occurs at the beginning of cell division when the basal body is duplicated, could be demonstrated in all the 8 T. cruzi isolates studied. This has been found in the human-pathogen strains as well as in the T. cruzi-like trypanosomes of wild animals. In contrast, in comparable developmental stages of T. conorhini, T. rangeli and two strains of T. lewisi, this structural configuration of the K-DNA could not be observed. Based on these results, the extent to which the central band of K-DNA may be used in differentiating between trypanosomes is discussed. These findings may also reflect the present state of knowledge, as based on the study of 12 trypanosome isolates, so that corrections or additions may be lateron possible."}
{"id": "PMID:1099749", "title": "A serological study on human Trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay.", "content": "A micro-scale enzyme linked immunosorbent assay for African trypanosomiasis is described. The test uses very small quantities of sera and with T. brucei antigen is sensitive in detecting T. rhodesiense infections in man. Cross reactions occur with antisera to, and antigens of, Leishmania donovani and Trypanosoma cruzi.", "contents": "A serological study on human Trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay. A micro-scale enzyme linked immunosorbent assay for African trypanosomiasis is described. The test uses very small quantities of sera and with T. brucei antigen is sensitive in detecting T. rhodesiense infections in man. Cross reactions occur with antisera to, and antigens of, Leishmania donovani and Trypanosoma cruzi."}
{"id": "PMID:1099750", "title": "[Antigenic translocation of the \"carcinoembryonic antigen\" in colonic neoplasms].", "content": "The localization of the carcinoembryonic antigen (CEA) in the colon tumours was studied with the indirect Coons test. The localization of CEA in malignant cells was different from that in the normal colon mucosa. In addition, an essential secretion of this antigen may occur in neoplasms. The alteretion of the antigen position in pathologically changed cells or its secretion, differing from that in normal tissue, is referred to as the antigenic translocation.", "contents": "[Antigenic translocation of the \"carcinoembryonic antigen\" in colonic neoplasms]. The localization of the carcinoembryonic antigen (CEA) in the colon tumours was studied with the indirect Coons test. The localization of CEA in malignant cells was different from that in the normal colon mucosa. In addition, an essential secretion of this antigen may occur in neoplasms. The alteretion of the antigen position in pathologically changed cells or its secretion, differing from that in normal tissue, is referred to as the antigenic translocation."}
{"id": "PMID:1099751", "title": "[Simple apparatus for drying cytological specimens by the \"critical point\" method].", "content": "A simple modification of the critical-point drying apparatus is described. This modification was effectively used for preparation of normal and malignant cell cultures using the scanning electron microscopy.", "contents": "[Simple apparatus for drying cytological specimens by the \"critical point\" method]. A simple modification of the critical-point drying apparatus is described. This modification was effectively used for preparation of normal and malignant cell cultures using the scanning electron microscopy."}
{"id": "PMID:1099765", "title": "Renal transplantation in the rat. I. Studies concerning the ureteral anastomosis with special reference to the end-to-end technique.", "content": "End-to-end anastomosis of the rat ureter over an in-dwelling silastic splint gives good primary results and a low frequency of complications. This technique has been used when reconstructing the ureter in situ and in renal transplantation.", "contents": "Renal transplantation in the rat. I. Studies concerning the ureteral anastomosis with special reference to the end-to-end technique. End-to-end anastomosis of the rat ureter over an in-dwelling silastic splint gives good primary results and a low frequency of complications. This technique has been used when reconstructing the ureter in situ and in renal transplantation."}
{"id": "PMID:1099766", "title": "Management of children with urinary tract infections: the Stanford experience.", "content": "Two hundred seventy-eight female children with urinary tract infections have been evaluated at Stanford division of urology. All children were followed up for a period of not less than twelve months. Age of onset of infection, clinical presentation, and nature of infecting organisms were observed. The group consisted of 144 children without ureteral reflux and 134 children with ureteral reflux. Sixty-one of the female children with ureteral reflux had ureteral reimplantation, while 73 received medical treatment alone. A study of infection rates in each of the three groups of children indicated a similar infection rate, although those children with reflux experienced a higher incidence of clinical pyelonephritis. Correction of ureteral reflux did not alter the infection rate; however, infections after surgical correction were generally of a type usually associated with children without reflux. Twenty-nine children had urethral dilatation, and the infection rate prior to and following urethral dilatation indicated a similar rate of infection pre- and posturethral dilatation. One hundred nonrefluxing kidneys were observed radiologically: 97 were normal and 3 showed clubbing and scarring. Of 110 refluxing renal units observed, 62 were clubbed and scarred and 48 were normal. Following surgical correction of reflux, renal clubbing and scarring were not observed in previously normal renal units. Of those renal units found to be abnormal at time of surgery, 66 per cent showed progression of clubbing and scarring after surgical correction of reflux. It was observed that the greater the degree of reflux present, the higher the incidence of renal damage. This study suggests that children who experience recurrent urinary tract infections who do not have ureteral reflux are seldom at renal risk; similar children who do have ureteral reflux are at risk unless the infections are controlled or the reflux either disappears or is corrected surgically.", "contents": "Management of children with urinary tract infections: the Stanford experience. Two hundred seventy-eight female children with urinary tract infections have been evaluated at Stanford division of urology. All children were followed up for a period of not less than twelve months. Age of onset of infection, clinical presentation, and nature of infecting organisms were observed. The group consisted of 144 children without ureteral reflux and 134 children with ureteral reflux. Sixty-one of the female children with ureteral reflux had ureteral reimplantation, while 73 received medical treatment alone. A study of infection rates in each of the three groups of children indicated a similar infection rate, although those children with reflux experienced a higher incidence of clinical pyelonephritis. Correction of ureteral reflux did not alter the infection rate; however, infections after surgical correction were generally of a type usually associated with children without reflux. Twenty-nine children had urethral dilatation, and the infection rate prior to and following urethral dilatation indicated a similar rate of infection pre- and posturethral dilatation. One hundred nonrefluxing kidneys were observed radiologically: 97 were normal and 3 showed clubbing and scarring. Of 110 refluxing renal units observed, 62 were clubbed and scarred and 48 were normal. Following surgical correction of reflux, renal clubbing and scarring were not observed in previously normal renal units. Of those renal units found to be abnormal at time of surgery, 66 per cent showed progression of clubbing and scarring after surgical correction of reflux. It was observed that the greater the degree of reflux present, the higher the incidence of renal damage. This study suggests that children who experience recurrent urinary tract infections who do not have ureteral reflux are seldom at renal risk; similar children who do have ureteral reflux are at risk unless the infections are controlled or the reflux either disappears or is corrected surgically."}
{"id": "PMID:1099768", "title": "Hypothalamic and pituitary function in hypogonadotropic hypogonadism.", "content": "Hypogonadotropic hypogonadism has been identified as a cause of partial or complete failure of puberty, may be familial and may have other associated abnormalities of hyposmia, intellectual retardation, perceptive deafness, color blindness, skeletal deformities, and gynecomastia. Pituitary function is usually normal with the primary defect believed to be hypothalamic. A twenty-year-old white male with a clinical diagnosis of hypogonadotropic hypogonadism and anosmia under-went complete endocrine evaluation with evaluation of the pituitary response to luteinizing hormone-releasing hormone. FSH (follicle-stimulating hormone) and LH (luteinizing hormone) release after luteinizing hormone-releasing hormone did occur, but the response was less than that seen in normal controls. Evaluation demonstrated that the pituitary-gonadal axis was intact with the hypothalamic-pituitary axis being defective. Therapy with the synthetic decapeptide (luteinizing hormone-releasing hormone) is correct theoretically and may be superior to therapy with exogenous gonadotropins.", "contents": "Hypothalamic and pituitary function in hypogonadotropic hypogonadism. Hypogonadotropic hypogonadism has been identified as a cause of partial or complete failure of puberty, may be familial and may have other associated abnormalities of hyposmia, intellectual retardation, perceptive deafness, color blindness, skeletal deformities, and gynecomastia. Pituitary function is usually normal with the primary defect believed to be hypothalamic. A twenty-year-old white male with a clinical diagnosis of hypogonadotropic hypogonadism and anosmia under-went complete endocrine evaluation with evaluation of the pituitary response to luteinizing hormone-releasing hormone. FSH (follicle-stimulating hormone) and LH (luteinizing hormone) release after luteinizing hormone-releasing hormone did occur, but the response was less than that seen in normal controls. Evaluation demonstrated that the pituitary-gonadal axis was intact with the hypothalamic-pituitary axis being defective. Therapy with the synthetic decapeptide (luteinizing hormone-releasing hormone) is correct theoretically and may be superior to therapy with exogenous gonadotropins."}
{"id": "PMID:1099770", "title": "Use of horseshoe kidney in renal transplantation: technical aspects.", "content": "The incidence and etiology of horseshoe kidneys and their structural abnormalities are discussed. Previously, patients with horseshoe kidneys have been considered undesirable donors for renal transplantation. Two cases of horseshoe kidney transplantation are presented, apparently, the first reported utilization of such a donor for transplantation, along with important aspects of surgical technique.", "contents": "Use of horseshoe kidney in renal transplantation: technical aspects. The incidence and etiology of horseshoe kidneys and their structural abnormalities are discussed. Previously, patients with horseshoe kidneys have been considered undesirable donors for renal transplantation. Two cases of horseshoe kidney transplantation are presented, apparently, the first reported utilization of such a donor for transplantation, along with important aspects of surgical technique."}
{"id": "PMID:1099771", "title": "Periureteric fibrosis complicating kidney transplantation.", "content": "Impairment of renal function developed in a kidney transplant recipient three years after the transplant due to ureteric obstruction. At operation this was found to be due to periureteric fibrosis. Ureterolysis was performed with subsequent return of renal function.", "contents": "Periureteric fibrosis complicating kidney transplantation. Impairment of renal function developed in a kidney transplant recipient three years after the transplant due to ureteric obstruction. At operation this was found to be due to periureteric fibrosis. Ureterolysis was performed with subsequent return of renal function."}
{"id": "PMID:1099808", "title": "Psychological sequelae of elective abortion.", "content": "A mild, short, depressive and guilt ridden period following abortion is quite common, but a severe psychological reaction is rare. The indication for the abortion and the preabortal psychological state of the patient are the two most important factors. Almost all reported instances of postabortion psychoses have occurred in patients who had severe preabortal psychiatric problems. Women undergoing abortion for socioeconomic or psychosocial indications appear to be at minimal risk for long-term negative psychological sequelae. In contrast, women in whom abortion is carried out because of exposure to rubella and the risk of fetal malformation, maternal organic disease or the prenatal diagnosis of a genetically defective fetus are at greater risk and may need supportive psychotherapy.", "contents": "Psychological sequelae of elective abortion. A mild, short, depressive and guilt ridden period following abortion is quite common, but a severe psychological reaction is rare. The indication for the abortion and the preabortal psychological state of the patient are the two most important factors. Almost all reported instances of postabortion psychoses have occurred in patients who had severe preabortal psychiatric problems. Women undergoing abortion for socioeconomic or psychosocial indications appear to be at minimal risk for long-term negative psychological sequelae. In contrast, women in whom abortion is carried out because of exposure to rubella and the risk of fetal malformation, maternal organic disease or the prenatal diagnosis of a genetically defective fetus are at greater risk and may need supportive psychotherapy."}
{"id": "PMID:1099804", "title": "[Stereotaxic longitudinal hippocampotomy and its prospects in the treatment of epilepsy].", "content": "A description is offered of a new method of stereotaxic longitudinal hippocampotomy that ensures an electrosubcorticographic precise location of an epileptogenic focus in the hippocampus and its selective destruction. A positive therapeutic effect was achieved in 13 patients operated upon by using this method.", "contents": "[Stereotaxic longitudinal hippocampotomy and its prospects in the treatment of epilepsy]. A description is offered of a new method of stereotaxic longitudinal hippocampotomy that ensures an electrosubcorticographic precise location of an epileptogenic focus in the hippocampus and its selective destruction. A positive therapeutic effect was achieved in 13 patients operated upon by using this method."}
{"id": "PMID:1099805", "title": "[Titration of antibodies to lymphocytic choriomeningitis virus by the method of indirect immunofluorescence].", "content": "Antibody to lymphocytic choriomeningitis virus was determined by the indirect immunofluorescence test in immune sera of guinea pigs and immune ascitic fluids of rats and mice. Among 135 patients with aseptic meningitis serum antibody was found in 11 patients in titers of 1 : 64 to 1 : 128 and in the cerebro-spinal fluid of these patients in considerably lower titers. By the indirect immunofluorescence test antibody in maximum titers was found early after the appearance of meningeal symptoms.", "contents": "[Titration of antibodies to lymphocytic choriomeningitis virus by the method of indirect immunofluorescence]. Antibody to lymphocytic choriomeningitis virus was determined by the indirect immunofluorescence test in immune sera of guinea pigs and immune ascitic fluids of rats and mice. Among 135 patients with aseptic meningitis serum antibody was found in 11 patients in titers of 1 : 64 to 1 : 128 and in the cerebro-spinal fluid of these patients in considerably lower titers. By the indirect immunofluorescence test antibody in maximum titers was found early after the appearance of meningeal symptoms."}
{"id": "PMID:1099823", "title": "Control of adenovirus acute respiratory disease in U.S. Army trainees.", "content": "Although limited almost exclusively to military trainees, acute respiratory disease (ARD) caused by adenovirus types 4 and 7 had been the leading cause of hospitalization in U.S. Army personnel. This decrease which resembles influenza in clinical manifestations led to hospitalization of as many as 50% of military trainees in midwinter and imposed a heavy burden on military hospitals and training programs. In studies undertaken from 1965 to 1970, live adenovirus type 4 and subsequently type 7 vaccines were found to be safe and immunogenic and to confer protection against type specific adenovirus ARD. For the past 5 yr. military trainees have been immunized with both adenovirus vaccines during periods of expected adenovirus disease. Since 1966, use of adenovirus vaccines has been monitored through the adenovirus surveillance program which yields weekly data on incidence and etiology of ARD in basic combat trainees. Since 1973, stable adenovirus vaccines have resulted in excellent control of adenovirus ARD. Potential problems with this immunization program are discussed.", "contents": "Control of adenovirus acute respiratory disease in U.S. Army trainees. Although limited almost exclusively to military trainees, acute respiratory disease (ARD) caused by adenovirus types 4 and 7 had been the leading cause of hospitalization in U.S. Army personnel. This decrease which resembles influenza in clinical manifestations led to hospitalization of as many as 50% of military trainees in midwinter and imposed a heavy burden on military hospitals and training programs. In studies undertaken from 1965 to 1970, live adenovirus type 4 and subsequently type 7 vaccines were found to be safe and immunogenic and to confer protection against type specific adenovirus ARD. For the past 5 yr. military trainees have been immunized with both adenovirus vaccines during periods of expected adenovirus disease. Since 1966, use of adenovirus vaccines has been monitored through the adenovirus surveillance program which yields weekly data on incidence and etiology of ARD in basic combat trainees. Since 1973, stable adenovirus vaccines have resulted in excellent control of adenovirus ARD. Potential problems with this immunization program are discussed."}
{"id": "PMID:1099824", "title": "An ultrastructural study of the renal medulla in experimental acute pyelonephritis.", "content": "Experimental acute pyelonephritis was produced in rats by a combination of intravenous administration of Escherichia coli, strain IMRU-54, and temporary unilateral mechanical ureteral obstruction. Structural alterations of the renal medulla were studied by light and electron microscopy. Major cellular alterations occurred in the vasa recta. Tubular and interstitial cells demonstrated minimal alterations after the brief period of acute inflammation. Polymorphonuclear leukocytes within tubular lumina contained structures resembling E. coli in nonprotoplasts-like form. Numerous protoplast-like organisms, to the exclusion of any other structural forms, were detected within the interstitium of the inner medulla. Nonprotoplast-like structures resembling E. coli were rarely observed in interstitium of the inner medulla. Following relief of ureteral obstruction, clearance of acute inflammation was rapid. In conclusion, hemoatogenous acute pyelonephritis induced by E. coli, IMRU-54, is able to inflict cytological and ultrastructural damage to structural elements of the inner and outer medulla of rats. Vasa recta incurred prominent alterations in endothelia and basement membranes, whereas tubular epithelia and interstitial cells had relatively good structural preservation. The data suggest that intravenously administered E. coli is capable to revert to a protoplast-like structure in the inner medulla.", "contents": "An ultrastructural study of the renal medulla in experimental acute pyelonephritis. Experimental acute pyelonephritis was produced in rats by a combination of intravenous administration of Escherichia coli, strain IMRU-54, and temporary unilateral mechanical ureteral obstruction. Structural alterations of the renal medulla were studied by light and electron microscopy. Major cellular alterations occurred in the vasa recta. Tubular and interstitial cells demonstrated minimal alterations after the brief period of acute inflammation. Polymorphonuclear leukocytes within tubular lumina contained structures resembling E. coli in nonprotoplasts-like form. Numerous protoplast-like organisms, to the exclusion of any other structural forms, were detected within the interstitium of the inner medulla. Nonprotoplast-like structures resembling E. coli were rarely observed in interstitium of the inner medulla. Following relief of ureteral obstruction, clearance of acute inflammation was rapid. In conclusion, hemoatogenous acute pyelonephritis induced by E. coli, IMRU-54, is able to inflict cytological and ultrastructural damage to structural elements of the inner and outer medulla of rats. Vasa recta incurred prominent alterations in endothelia and basement membranes, whereas tubular epithelia and interstitial cells had relatively good structural preservation. The data suggest that intravenously administered E. coli is capable to revert to a protoplast-like structure in the inner medulla."}
{"id": "PMID:1099826", "title": "[Stress sensitivity and adaptation].", "content": "On the basis of theoretical, clinical and experimental analyses the influence of different stress categories, especially of psycho-emotional and socio-emotional stress, upon cerebro-visceral regulation processes is investigated. Central-nervous information processing in various brain areas (neocortex, limbic system, hypothalamus, formatio reticularis mesencephali etc.) and learning processes are equally affected by stress factors as are haemodynamic and biochemical parameters. Changes of the carbohydrate and lipid metabolism, of plasma renin activity, catechol amine release and the counter-regulatory insulin enhancement are especially pronounced in \"stress-sensitive risk persons\", e.g. in the hypertensive as a maladaptive dysregulation-as opposed to the adaptive regulation capacity of healthy individuals. The investigated functional patterns of different systems of the organism allow the distinction of hyper- and hyporeactive forms of maladaptation. The pathogenetic efficiency of stress is determined not only by the quantity, quality, duration of exposure and basic processes of the central nervous system, but also by man's personality structure and his sensitivity to stress (genetic predisposition and/or acquired maladaptation). More insight into the neuro-biological processes underlying the motivational and psycho-emotional performance of the brain and its connection withe humoral-hormonal and cardiovascular systems is necessary to treat and to control cerebrovisceral dysregulations such as arterial essential hypertension. It will be a task of psychotherapy to help improve man's adaptive capacity and to desensibilize stress-sensitive risk persons.", "contents": "[Stress sensitivity and adaptation]. On the basis of theoretical, clinical and experimental analyses the influence of different stress categories, especially of psycho-emotional and socio-emotional stress, upon cerebro-visceral regulation processes is investigated. Central-nervous information processing in various brain areas (neocortex, limbic system, hypothalamus, formatio reticularis mesencephali etc.) and learning processes are equally affected by stress factors as are haemodynamic and biochemical parameters. Changes of the carbohydrate and lipid metabolism, of plasma renin activity, catechol amine release and the counter-regulatory insulin enhancement are especially pronounced in \"stress-sensitive risk persons\", e.g. in the hypertensive as a maladaptive dysregulation-as opposed to the adaptive regulation capacity of healthy individuals. The investigated functional patterns of different systems of the organism allow the distinction of hyper- and hyporeactive forms of maladaptation. The pathogenetic efficiency of stress is determined not only by the quantity, quality, duration of exposure and basic processes of the central nervous system, but also by man's personality structure and his sensitivity to stress (genetic predisposition and/or acquired maladaptation). More insight into the neuro-biological processes underlying the motivational and psycho-emotional performance of the brain and its connection withe humoral-hormonal and cardiovascular systems is necessary to treat and to control cerebrovisceral dysregulations such as arterial essential hypertension. It will be a task of psychotherapy to help improve man's adaptive capacity and to desensibilize stress-sensitive risk persons."}
{"id": "PMID:1099827", "title": "[Determination of kidney circulation with radioxenon].", "content": "On 23 investigations in dogs the 133xenon eluation method was compared with the bloody measurement of the renal blood supply. The most favourable correlation presents the measurement of the initial decrease of the curves with r = 0.827 and y = 0.889x + 53.3, besides the modified hight-surface-method. Possibilities of error exist especially by the recirculation of xenon and the influence on the blood supply by the xenon-injection.", "contents": "[Determination of kidney circulation with radioxenon]. On 23 investigations in dogs the 133xenon eluation method was compared with the bloody measurement of the renal blood supply. The most favourable correlation presents the measurement of the initial decrease of the curves with r = 0.827 and y = 0.889x + 53.3, besides the modified hight-surface-method. Possibilities of error exist especially by the recirculation of xenon and the influence on the blood supply by the xenon-injection."}
{"id": "PMID:1099828", "title": "[Mumps myocarditis in a small child].", "content": "The clinical history and post-mortem findings in a five-year-old girl are reported. She died in consequence of a chronic mumps myocarditis 13 weeks after having fallen ill with mumps. The antigen specific for mumps virus could be demonstrated in the myocardium by means of immunofluorescence.", "contents": "[Mumps myocarditis in a small child]. The clinical history and post-mortem findings in a five-year-old girl are reported. She died in consequence of a chronic mumps myocarditis 13 weeks after having fallen ill with mumps. The antigen specific for mumps virus could be demonstrated in the myocardium by means of immunofluorescence."}
{"id": "PMID:1099829", "title": "A selective coronary indicator dilution technique with fiberoptic recording.", "content": "A new method of accurately measuring myocardial circulation times is described: dye injections were made selectively into the left coronary artery while the passage of dye was recorded instantaneously in the coronary sinus with a fiberoptic catheter. The appearance time was found to be shorter than previously estimated (2.2 +/- 0.8 sec). The mean transit time in patients with left coronary artery disease and/or myocardial disease was significantly prolonged (p less than 0.01) and was found in all patients to shorten with increasing heart rate (p less than 0.01). Deformed dyd dilution curves were only obtained with stenotic lesions of the left coronary artery.", "contents": "A selective coronary indicator dilution technique with fiberoptic recording. A new method of accurately measuring myocardial circulation times is described: dye injections were made selectively into the left coronary artery while the passage of dye was recorded instantaneously in the coronary sinus with a fiberoptic catheter. The appearance time was found to be shorter than previously estimated (2.2 +/- 0.8 sec). The mean transit time in patients with left coronary artery disease and/or myocardial disease was significantly prolonged (p less than 0.01) and was found in all patients to shorten with increasing heart rate (p less than 0.01). Deformed dyd dilution curves were only obtained with stenotic lesions of the left coronary artery."}
{"id": "PMID:1099830", "title": "[Sinus node syndrome].", "content": "Recently disorders of sinus node function have found increasing interest in clinical medicine thanks to new diagnostic and therapeutic developments. This paper represents a comprehensive review of these conditions, combined under the name \"Sick Sinus Syndrome\" (SSS). Besides a detailed analysis of 63 cases seen at our institution, the results of other groups are compared and extensively discussed. The clinical picture of the SSS is characterized by a wide variety of bradycardiac and tachycardic atrial arrhythmias, occurring separately or in combination. These can be classified in three subgroups: Patients with exclusive sinus bradycardia; patients with sinoatrial exit block or transient episodes of sinus arrest with or without AV escape rhythms; and finally patients with the bradycardia/tachycardia-syndrome, which are complicated by additional atrial tachyarrhythmias. The symptomatology of the SSS is multiform and extends from symptomless cases and those with only general signs of reduced cardiac function to patients with recurrent severe syncopal attacks which may lead to cerebral damage and even death. Besides the typical history, the diagnosis of the SSS primarily rests upon the ECG, especially the long term ECG recorded continuously on a 24 hrs. tape (Holter technique). Also the exercise ECG is of some value, characteristically showing an inadequate increase in the sinus rate, sometimes with AV escape systoles and -rhythms. In addition various provocative tests have been devised which are of help to differentiate between a pathologic and a normal sinus node function. Among these the determination of the sinus node recovery time following overdrive atrial pacing has gained wide acceptance. In most cases the exact etiology of the SSS is not known. In addition to coronary and inflammatory heart diseases a primarily degenerative lesion of the sinus node, comparable to cases with \"primary heart block\" are discussed. There is also a remarkably frequent past history diththeria. Rarer causes of the condition represent cases with cardiomyopathy, thyreotoxic heart disease, collagen and other disorders and also a familial manifestation of the SSS has been described. Therapeutically, pharmacologic treatment with vagolytic, beta-adrenergic or the common antiarrhythmic drugs is often unsuccessful, especially in the treatment of the Brady-Tachy-Syndrome. Digitalis glycosides, however, are frequently of some value, as they represent an effective prophylactic agent against atrial tachyarrhythmias without prolonging the sinus node recovery time or reducing significantly the sinus rate. While a few patients do not require any treatment, an artificial cardiac pacemaker has to be inserted in most cases. Atrial stimulation may be superior to ventricular on-demand pacing in some patients, and also a special system for the treatment of the SSS combined with significant AV block (binodal disease) has been designed, the bifocal sequential pacemaker.", "contents": "[Sinus node syndrome]. Recently disorders of sinus node function have found increasing interest in clinical medicine thanks to new diagnostic and therapeutic developments. This paper represents a comprehensive review of these conditions, combined under the name \"Sick Sinus Syndrome\" (SSS). Besides a detailed analysis of 63 cases seen at our institution, the results of other groups are compared and extensively discussed. The clinical picture of the SSS is characterized by a wide variety of bradycardiac and tachycardic atrial arrhythmias, occurring separately or in combination. These can be classified in three subgroups: Patients with exclusive sinus bradycardia; patients with sinoatrial exit block or transient episodes of sinus arrest with or without AV escape rhythms; and finally patients with the bradycardia/tachycardia-syndrome, which are complicated by additional atrial tachyarrhythmias. The symptomatology of the SSS is multiform and extends from symptomless cases and those with only general signs of reduced cardiac function to patients with recurrent severe syncopal attacks which may lead to cerebral damage and even death. Besides the typical history, the diagnosis of the SSS primarily rests upon the ECG, especially the long term ECG recorded continuously on a 24 hrs. tape (Holter technique). Also the exercise ECG is of some value, characteristically showing an inadequate increase in the sinus rate, sometimes with AV escape systoles and -rhythms. In addition various provocative tests have been devised which are of help to differentiate between a pathologic and a normal sinus node function. Among these the determination of the sinus node recovery time following overdrive atrial pacing has gained wide acceptance. In most cases the exact etiology of the SSS is not known. In addition to coronary and inflammatory heart diseases a primarily degenerative lesion of the sinus node, comparable to cases with \"primary heart block\" are discussed. There is also a remarkably frequent past history diththeria. Rarer causes of the condition represent cases with cardiomyopathy, thyreotoxic heart disease, collagen and other disorders and also a familial manifestation of the SSS has been described. Therapeutically, pharmacologic treatment with vagolytic, beta-adrenergic or the common antiarrhythmic drugs is often unsuccessful, especially in the treatment of the Brady-Tachy-Syndrome. Digitalis glycosides, however, are frequently of some value, as they represent an effective prophylactic agent against atrial tachyarrhythmias without prolonging the sinus node recovery time or reducing significantly the sinus rate. While a few patients do not require any treatment, an artificial cardiac pacemaker has to be inserted in most cases. Atrial stimulation may be superior to ventricular on-demand pacing in some patients, and also a special system for the treatment of the SSS combined with significant AV block (binodal disease) has been designed, the bifocal sequential pacemaker."}
{"id": "PMID:1099831", "title": "[Antiarrhythmic properties of lidoflazine in the therapy of auricular fibrillation and flutter (clinical studies of 26 patients)].", "content": "Successful conversion to sinus rhythm was achieved in 20 out of 26 patients (77%) with chronic atrial fibrillation or flutter of various origin, that had existed for about 40 +/- 13 months. There was a significant relation between the converting dose of Lidoflazine and the duration of atrial fibrillation. In those patients with atrial fibrillation, that preexisted no longer than two years, restoration of sinus rhythm was achieved in 12 of 14 cases (86%) with an average dose of 109 +/- 67 mg Lidoflazine a day, whereas in the group with atrial fibrillation known for more than two years therapeutical success was obtained only in 8 of 12 cases (67%). The dose of conversion was 382 +/- 150 mg/day in this group and produced in a few patients toxic side effects. Two patients relapsed to atrial fibrillation. On this reasons conversion with Lidoflazine should only be done in patients with atrial fibrillation within two years. There were no significant changes related to: heart rate, myocardial function and intraventricular conduction process. In the presence of underlying hypokalaemic disturbances and/or additional therapy with digitalis preparations Lidoflazine may be the cause of developing premature beats. Lidoflazine may be regarded as a relatively safe drug even in large doses, if monitoring of heart rhythm and QTc-interval is available.", "contents": "[Antiarrhythmic properties of lidoflazine in the therapy of auricular fibrillation and flutter (clinical studies of 26 patients)]. Successful conversion to sinus rhythm was achieved in 20 out of 26 patients (77%) with chronic atrial fibrillation or flutter of various origin, that had existed for about 40 +/- 13 months. There was a significant relation between the converting dose of Lidoflazine and the duration of atrial fibrillation. In those patients with atrial fibrillation, that preexisted no longer than two years, restoration of sinus rhythm was achieved in 12 of 14 cases (86%) with an average dose of 109 +/- 67 mg Lidoflazine a day, whereas in the group with atrial fibrillation known for more than two years therapeutical success was obtained only in 8 of 12 cases (67%). The dose of conversion was 382 +/- 150 mg/day in this group and produced in a few patients toxic side effects. Two patients relapsed to atrial fibrillation. On this reasons conversion with Lidoflazine should only be done in patients with atrial fibrillation within two years. There were no significant changes related to: heart rate, myocardial function and intraventricular conduction process. In the presence of underlying hypokalaemic disturbances and/or additional therapy with digitalis preparations Lidoflazine may be the cause of developing premature beats. Lidoflazine may be regarded as a relatively safe drug even in large doses, if monitoring of heart rhythm and QTc-interval is available."}
{"id": "PMID:1099848", "title": "[Newer antimycotics. I. Derivatives of phenyl-hydrazine (author's transl)].", "content": "The author produced a number of derivatives of phenyl-hydrazine and its analogues, investigated their antimicrobial efficiency in vitro, and discussed the association of chemical structure with the fungistatic effect in the series of phenyl-hydrazine-derivatives. From the author's results it can be concluded that the halogen-substituted phenyl-hydrazines and their N-(acetyl)-derivatives exert a moderate fungistatic effect (Tab. 1). In the series of N-aryl-sulphonyl-N'-phenyl-hydrazines only the compounds derived from 3,4-dichloro-phenyl-hydrazine proved to be effective; the other representatives of this type were fully ineffective (Tab. 1). The majority of the halogen-substituted N-aroyl-N'-phenyl-hydrazines exerted a relatively strong fungistatic activity with a broad spectrum of efficiency (Tab. 2). On the contrary, their analogues having a -CO-NH- group in place of the -CO-NH-NH-group, were fully inactive (Tab. 3). From the investigated derivatives of phenyl-hydrazine, the N-benzoyl-, N-(2-chloro-benzoyl)-, N-(4-chloro-benzoyl)- and N-furoyl-derivatives of 3- and 4-chloro-phenyl-hydrazine, furthermore the N-benzoyl- and N-furoyl-derivatives of 3,4-dichloro-phenyl-hydrazine were most effective (Tab. 2). The acute toxicity in the mouse of these effective compounds was relatively small; they were found to be significantly less toxic than tetramethyl-thiuramdisulfide (Tab. 4). On the basis of an intensive fungistatic efficiency and small toxicity of the halogen-substituted N-aroyl-N'-phenyl-hydrazines, it seems that a number of representatives of these types of chemical compounds could be given a role in the chemotherapy of superficial human and animal dermatomycoses, and also in the chemoprophylaxis of certain phytomycoses.", "contents": "[Newer antimycotics. I. Derivatives of phenyl-hydrazine (author's transl)]. The author produced a number of derivatives of phenyl-hydrazine and its analogues, investigated their antimicrobial efficiency in vitro, and discussed the association of chemical structure with the fungistatic effect in the series of phenyl-hydrazine-derivatives. From the author's results it can be concluded that the halogen-substituted phenyl-hydrazines and their N-(acetyl)-derivatives exert a moderate fungistatic effect (Tab. 1). In the series of N-aryl-sulphonyl-N'-phenyl-hydrazines only the compounds derived from 3,4-dichloro-phenyl-hydrazine proved to be effective; the other representatives of this type were fully ineffective (Tab. 1). The majority of the halogen-substituted N-aroyl-N'-phenyl-hydrazines exerted a relatively strong fungistatic activity with a broad spectrum of efficiency (Tab. 2). On the contrary, their analogues having a -CO-NH- group in place of the -CO-NH-NH-group, were fully inactive (Tab. 3). From the investigated derivatives of phenyl-hydrazine, the N-benzoyl-, N-(2-chloro-benzoyl)-, N-(4-chloro-benzoyl)- and N-furoyl-derivatives of 3- and 4-chloro-phenyl-hydrazine, furthermore the N-benzoyl- and N-furoyl-derivatives of 3,4-dichloro-phenyl-hydrazine were most effective (Tab. 2). The acute toxicity in the mouse of these effective compounds was relatively small; they were found to be significantly less toxic than tetramethyl-thiuramdisulfide (Tab. 4). On the basis of an intensive fungistatic efficiency and small toxicity of the halogen-substituted N-aroyl-N'-phenyl-hydrazines, it seems that a number of representatives of these types of chemical compounds could be given a role in the chemotherapy of superficial human and animal dermatomycoses, and also in the chemoprophylaxis of certain phytomycoses."}
{"id": "PMID:1099849", "title": "[On the morphology of flagella and fimbriae of Escherichia strains (author's transl)].", "content": "100 Escherichia-strains, isolated from urine and faeces, were investigated with regard to flagella and fimbriae by electron-microskopy. The flagella had a length of 3-6 mu and a diameter of ca 0.02 mu. The flagellation was different not only between to the single bacteria of the same strain, but also in the separated isolates. Of 50 strains from urine had 27 and of 50 strains from feces had 28 no flagella. Different Escherichia-strains contained fimbriae, the number of which was much higher with regard to the flagella. The length of the fimbriae differed between 0.25 and 2 mu, the diameter was constantly ca 8 nm. 21 strains of 100 (4 from 50 urine strains and 17 from 50 faeces strains) had no fimbriae.", "contents": "[On the morphology of flagella and fimbriae of Escherichia strains (author's transl)]. 100 Escherichia-strains, isolated from urine and faeces, were investigated with regard to flagella and fimbriae by electron-microskopy. The flagella had a length of 3-6 mu and a diameter of ca 0.02 mu. The flagellation was different not only between to the single bacteria of the same strain, but also in the separated isolates. Of 50 strains from urine had 27 and of 50 strains from feces had 28 no flagella. Different Escherichia-strains contained fimbriae, the number of which was much higher with regard to the flagella. The length of the fimbriae differed between 0.25 and 2 mu, the diameter was constantly ca 8 nm. 21 strains of 100 (4 from 50 urine strains and 17 from 50 faeces strains) had no fimbriae."}
{"id": "PMID:1099850", "title": "[On the biological activity of Escherichia strains, with special reference to fimbriae (author's transl)].", "content": "According to the results of earlier investigations of other authors, there are differences between Escherichia-strains fron urinary tract infections and fron the feces of healthy persons with regard to their biochemical, hemolytic, necrotizing and pathogenic properties in mice experiments. Hemolytic Escherichia cultures are very different from non - hemolytic cultures in their necrotizing and pathogenic behaviour. In contrast to Escherichia-strains from the feces of healthy persons strains from urinary tract infections have fimbriae to a much greater extent and hemolytic strains up to 100%.", "contents": "[On the biological activity of Escherichia strains, with special reference to fimbriae (author's transl)]. According to the results of earlier investigations of other authors, there are differences between Escherichia-strains fron urinary tract infections and fron the feces of healthy persons with regard to their biochemical, hemolytic, necrotizing and pathogenic properties in mice experiments. Hemolytic Escherichia cultures are very different from non - hemolytic cultures in their necrotizing and pathogenic behaviour. In contrast to Escherichia-strains from the feces of healthy persons strains from urinary tract infections have fimbriae to a much greater extent and hemolytic strains up to 100%."}
{"id": "PMID:1099851", "title": "[Is the examination of drinkable water and swimming pools on presence of enteropathogenic E. coli necessary? (author's transl)].", "content": "The examination of 1950 different water samples showed that 21,5 percent of them were microbiologically not safe and had an high MPN of E. coli, 2,3 percent of these strains belonged to enteropathogenic groups. The percentage depended on the character of water establishment, and was in small water supplies as high as 8 percent, in swimming pools 11 percent. There were identified the following OK groups of EEC: O25:K11, O28:K73, O55:K59, O75:K80, O115, O126:K71, O146, which are well known as causes of acute enteric diseases of children and adults. In case of special epidemiological or other occasions, it would be useful to examine the E. coli strains, isolated from different water samples serologically, in order to identify them as enteropathogenic eventually.", "contents": "[Is the examination of drinkable water and swimming pools on presence of enteropathogenic E. coli necessary? (author's transl)]. The examination of 1950 different water samples showed that 21,5 percent of them were microbiologically not safe and had an high MPN of E. coli, 2,3 percent of these strains belonged to enteropathogenic groups. The percentage depended on the character of water establishment, and was in small water supplies as high as 8 percent, in swimming pools 11 percent. There were identified the following OK groups of EEC: O25:K11, O28:K73, O55:K59, O75:K80, O115, O126:K71, O146, which are well known as causes of acute enteric diseases of children and adults. In case of special epidemiological or other occasions, it would be useful to examine the E. coli strains, isolated from different water samples serologically, in order to identify them as enteropathogenic eventually."}
{"id": "PMID:1099852", "title": "[Comparative investigations on the efficacy of tap water, some detergents and ethanol on the transient flora of the hands (author's transl)].", "content": "The germ reducing efficacy of tap water, soap, pHisoHex, pHisoDerm and 70% ethanol was compared using the model of hands artificially contaminated with E. coli. Each of ten persons washed 1, 2 or 4 minutes. Reduction factors were calculated from pre- and post-values and were compared for each individual. After one minute the logarithms of the geometric means of the reduction factors amounted to 2,99, 3,23, 3,28, 3,61, 4,25 in the above used sequence (Table 2). After one minute the reductions came off more slowly and the slopes were linear and parallel as far as the mechanical germ reducing procedures are concerned (Fig. 2). In contrast, ethanol caused a steeper slope even after one minute wash. The strong reduction achieved by mechanical procedures (even with water for one minute 3 powers of ten were observed) demonstrates the high hygienic importance of hand washings for removal of the transient flora. Detergents like pHisoDerm produce further significantly higher reductions and seem to be more suitable than aggressive disinfectants for application in fields where bactericidal action is not imperative. The proposals for procedures aiming at disinfecting of the transient flora issued by the Austrian Society for Hygiene, Microbiology and Preventive Medicine require a reduction factor of at least 10(5) after two minutes hand wash. The results of this investigation show that this is practicable with ethanol and necessary, otherwise the bactericidal action would not be different from a mechanical removal of the transient flora.", "contents": "[Comparative investigations on the efficacy of tap water, some detergents and ethanol on the transient flora of the hands (author's transl)]. The germ reducing efficacy of tap water, soap, pHisoHex, pHisoDerm and 70% ethanol was compared using the model of hands artificially contaminated with E. coli. Each of ten persons washed 1, 2 or 4 minutes. Reduction factors were calculated from pre- and post-values and were compared for each individual. After one minute the logarithms of the geometric means of the reduction factors amounted to 2,99, 3,23, 3,28, 3,61, 4,25 in the above used sequence (Table 2). After one minute the reductions came off more slowly and the slopes were linear and parallel as far as the mechanical germ reducing procedures are concerned (Fig. 2). In contrast, ethanol caused a steeper slope even after one minute wash. The strong reduction achieved by mechanical procedures (even with water for one minute 3 powers of ten were observed) demonstrates the high hygienic importance of hand washings for removal of the transient flora. Detergents like pHisoDerm produce further significantly higher reductions and seem to be more suitable than aggressive disinfectants for application in fields where bactericidal action is not imperative. The proposals for procedures aiming at disinfecting of the transient flora issued by the Austrian Society for Hygiene, Microbiology and Preventive Medicine require a reduction factor of at least 10(5) after two minutes hand wash. The results of this investigation show that this is practicable with ethanol and necessary, otherwise the bactericidal action would not be different from a mechanical removal of the transient flora."}
{"id": "PMID:1099857", "title": "[Measurement of nuclear size in cells from embryonic mouse brain in monolayer culture using automatic image analysers].", "content": "Size analyses and size classifications were made of the nuclei of embryonal mouse brain cells in monolayer culture. Measurements were made by surface analysis, using the Quantimet (1,299 nuclei), and linear analysis, using the Digiscan (1,887 nuclei). Despite the different methods of measurement and different logarithmic classification, similar distribution curves were obtained, approaching the Gaussian normal distribution. The methodical problems of karyometry as well as the problems of statistical data processing are discussed.", "contents": "[Measurement of nuclear size in cells from embryonic mouse brain in monolayer culture using automatic image analysers]. Size analyses and size classifications were made of the nuclei of embryonal mouse brain cells in monolayer culture. Measurements were made by surface analysis, using the Quantimet (1,299 nuclei), and linear analysis, using the Digiscan (1,887 nuclei). Despite the different methods of measurement and different logarithmic classification, similar distribution curves were obtained, approaching the Gaussian normal distribution. The methodical problems of karyometry as well as the problems of statistical data processing are discussed."}
{"id": "PMID:1099858", "title": "Observations on the intrinsic innervation of the islet of Langerhans of Rattus rattus rufescens (Indian black rat).", "content": "The innervation of Langerhans' islets appeared rather profuse. Generally the nerve fibres of the peri-insular plexus formed a basket enveloping the islet of Langerhans. The peri-insular ganglia were occasionally seen. No autonomic interstitial cells of Cajal nor nerve endings were observed in the islet of Langerhans, due to the thickness of the sections. The 'complexes sympathico-neuro-insulaires' were occasionally seen. The plexuses and neural terminal network were seen connected to each other; thus they are not separate entities.", "contents": "Observations on the intrinsic innervation of the islet of Langerhans of Rattus rattus rufescens (Indian black rat). The innervation of Langerhans' islets appeared rather profuse. Generally the nerve fibres of the peri-insular plexus formed a basket enveloping the islet of Langerhans. The peri-insular ganglia were occasionally seen. No autonomic interstitial cells of Cajal nor nerve endings were observed in the islet of Langerhans, due to the thickness of the sections. The 'complexes sympathico-neuro-insulaires' were occasionally seen. The plexuses and neural terminal network were seen connected to each other; thus they are not separate entities."}
{"id": "PMID:1099859", "title": "Serum lipids during oestradiol-valerate/norgestrel treatment of menopausal women. Double-blind study of a sequential preparation.", "content": "Thirty-three women aged 38-56 years, all with menopausal complaints, were treated after randomization during three cycles either with oestradiolvalerate/norgestrel suquential therapy or a placebo and then during three cycles with the opposite drug. Serum cholesterol, triglycerides, glycerol as well as plasma FFA and lipoprotein electrophoresis were studied before the institution of treatment, after each cycle, and 4 weeks after the intake of the last tablet. During the treatment period the triglyceride level was significantly lower than during the placebo period, whereas no changes occurred in other parameters. The decrease was at a maximum after 8 weeks' treatment; 4 weeks after the discontinuation of treatment the levels were as before its institution. It cannot be decided whether the decrease was due to the natural oestrogen or to the gestagen.", "contents": "Serum lipids during oestradiol-valerate/norgestrel treatment of menopausal women. Double-blind study of a sequential preparation. Thirty-three women aged 38-56 years, all with menopausal complaints, were treated after randomization during three cycles either with oestradiolvalerate/norgestrel suquential therapy or a placebo and then during three cycles with the opposite drug. Serum cholesterol, triglycerides, glycerol as well as plasma FFA and lipoprotein electrophoresis were studied before the institution of treatment, after each cycle, and 4 weeks after the intake of the last tablet. During the treatment period the triglyceride level was significantly lower than during the placebo period, whereas no changes occurred in other parameters. The decrease was at a maximum after 8 weeks' treatment; 4 weeks after the discontinuation of treatment the levels were as before its institution. It cannot be decided whether the decrease was due to the natural oestrogen or to the gestagen."}
{"id": "PMID:1099860", "title": "Diazepam as a sedative in induced abortion.", "content": "In 136 patients, who underwent induced abortion by the vacuum curettage method under local anaesthesia (paracervical block), the effect of 10 mg diazepam intravenously as preoperative sedative was investigated for its ability to abolish the subjective experience of pain. The trial was carried out as a paired sample, random, allocation, double-blind, fixed dose trial, and the statistical method was sequential analysis. Thirty-six pairs showed no difference and were excluded. After 32 pairs (24 A preferences and 8 O preferences), diazepam showed a significant superiority to placebo (p less than 0.05) (Fig. 1.) Amnesia for the procedure was reported by about one-quarter of the diazepam-treated patients, and in practically all cases the sedative effect had subsided 1-3 hours after the operation.", "contents": "Diazepam as a sedative in induced abortion. In 136 patients, who underwent induced abortion by the vacuum curettage method under local anaesthesia (paracervical block), the effect of 10 mg diazepam intravenously as preoperative sedative was investigated for its ability to abolish the subjective experience of pain. The trial was carried out as a paired sample, random, allocation, double-blind, fixed dose trial, and the statistical method was sequential analysis. Thirty-six pairs showed no difference and were excluded. After 32 pairs (24 A preferences and 8 O preferences), diazepam showed a significant superiority to placebo (p less than 0.05) (Fig. 1.) Amnesia for the procedure was reported by about one-quarter of the diazepam-treated patients, and in practically all cases the sedative effect had subsided 1-3 hours after the operation."}
{"id": "PMID:1099854", "title": "Computer discrimination between benign and malignant urothelial cells.", "content": "Computer discrimination between benign and malignant cells by means of the TICAS routines and subroutines has been accomplished with a small error of classification, not greater than ten percent. One feature of major diagnostic significance, namely, nuclear texture was singled out for discussion. This study augurs well for the future of computerized cytology of the urinary tract.", "contents": "Computer discrimination between benign and malignant urothelial cells. Computer discrimination between benign and malignant cells by means of the TICAS routines and subroutines has been accomplished with a small error of classification, not greater than ten percent. One feature of major diagnostic significance, namely, nuclear texture was singled out for discussion. This study augurs well for the future of computerized cytology of the urinary tract."}
{"id": "PMID:1099865", "title": "Influence of absorbed dose and field size on the geometry of the radiation-surgical brain lesion.", "content": "Specimens from a number of patients locally irradiated in the thalamus for intractable pain with a multiple gamma beam technique have been examined. The geometry of well circumscribed necrotic lesions was ascertained. No correlation was seen between the dimensions of the lesions and the size of the field, 3 mm X 5 mm or 3 mm X 7 mm, within the clinically useful dose range, 16 to 25 krad.", "contents": "Influence of absorbed dose and field size on the geometry of the radiation-surgical brain lesion. Specimens from a number of patients locally irradiated in the thalamus for intractable pain with a multiple gamma beam technique have been examined. The geometry of well circumscribed necrotic lesions was ascertained. No correlation was seen between the dimensions of the lesions and the size of the field, 3 mm X 5 mm or 3 mm X 7 mm, within the clinically useful dose range, 16 to 25 krad."}
{"id": "PMID:1099866", "title": "Destruction of small intracranial tumours with 60Co gamma radiation. Physical and technical considerations.", "content": "The clinical and physical conditions for the destruction of small intracranial tumours by means of multiple gamma beam irradiation are evaluated. The effects of the collimation of the single beam and the spatial distribution of 179 beams on the superimposed three-dimensional dose distribution are investigated experimentally and theoretically. Techniques for selective irradiation of tumours of various types and sizes are discussed.", "contents": "Destruction of small intracranial tumours with 60Co gamma radiation. Physical and technical considerations. The clinical and physical conditions for the destruction of small intracranial tumours by means of multiple gamma beam irradiation are evaluated. The effects of the collimation of the single beam and the spatial distribution of 179 beams on the superimposed three-dimensional dose distribution are investigated experimentally and theoretically. Techniques for selective irradiation of tumours of various types and sizes are discussed."}
{"id": "PMID:1099867", "title": "Errors and uncertainties in external radiation therapy. A system analysis with a cell kinetic model.", "content": "The relative importance of accurate knowledge and control of various biologic and physical factors in external radiation therapy was estimated with a simple cell kinetic treatment response model for a simulated tumour therapy situation. A set of parameter values was chosen for a reference case so that the calculated probability of zero surviving tumour cells was about 0.5. The parameters were varied, one at a time, over a range that was considered representative with respect to uncertainty in dose calculation, dose delivery and the description of the pertinent patient data. Also parameters in the cell kinetic model were varied. The calculations demonstrated a strong influence on the number of surviving cells from even moderate changes in the parameters which affect the tumour dose. Predictions with regard to curability are heavily dependent on the reliability of the biologic parameters in the model.", "contents": "Errors and uncertainties in external radiation therapy. A system analysis with a cell kinetic model. The relative importance of accurate knowledge and control of various biologic and physical factors in external radiation therapy was estimated with a simple cell kinetic treatment response model for a simulated tumour therapy situation. A set of parameter values was chosen for a reference case so that the calculated probability of zero surviving tumour cells was about 0.5. The parameters were varied, one at a time, over a range that was considered representative with respect to uncertainty in dose calculation, dose delivery and the description of the pertinent patient data. Also parameters in the cell kinetic model were varied. The calculations demonstrated a strong influence on the number of surviving cells from even moderate changes in the parameters which affect the tumour dose. Predictions with regard to curability are heavily dependent on the reliability of the biologic parameters in the model."}
{"id": "PMID:1099878", "title": "Postoperative ventriculitis in infants.", "content": "Eight consecutive cases of ventriculitis detected and treated during a four year period are reviewed. This complication represented 7.9% of all cases of spina bifida treated surgically, and 5.4% of all shunt precedures performed during the study period. Prophylactic antibiotic treatment did not reduce the incidence of ventriculitis, and in fact seemed to produce resistant strains, which led to difficulties in treatment and a worse prognosis. The presence of an intraventricular foreign body precludes permanent sterilization of the ventricular fluid. Finally, in those cases in which the antibiotic of choice penetrates poorly into the cerebrospinal fluid; intraventricular as well as systemic administration of the drug is indicated. With Gram negative organisms, Gentamicin is currently the drug of choice.", "contents": "Postoperative ventriculitis in infants. Eight consecutive cases of ventriculitis detected and treated during a four year period are reviewed. This complication represented 7.9% of all cases of spina bifida treated surgically, and 5.4% of all shunt precedures performed during the study period. Prophylactic antibiotic treatment did not reduce the incidence of ventriculitis, and in fact seemed to produce resistant strains, which led to difficulties in treatment and a worse prognosis. The presence of an intraventricular foreign body precludes permanent sterilization of the ventricular fluid. Finally, in those cases in which the antibiotic of choice penetrates poorly into the cerebrospinal fluid; intraventricular as well as systemic administration of the drug is indicated. With Gram negative organisms, Gentamicin is currently the drug of choice."}
{"id": "PMID:1099886", "title": "Frank vectorcardiogram in common ventricle: correlation with anatomic findings.", "content": "The influence on the Frank vectorcardiogram of anatomic features in common ventricle was analyzed by comparison of Frank vectorcardiograms in 51 patients who had common ventricle with those of 36 patients who had large ventricular septal defect, 14 of whom had levotransposition of the great arteries and 22 of whom had dextrotransposition. Frank vector loops in common ventricle differed from those in ventricular septal defect by the frequent occurrence of clockwise horizontal rotation, most common in patients with type C common ventricle (no outflow chamber). There was a statistically significant difference in the amplitude of early forces between patients with common ventricle and those with large ventricular septal defect. The degree of overlap of vectorcardiographic features between the two groups is sufficiently great that the vectorcardiogram has limited usefulness in establishing the diagnosis of common ventricle in any individual patient.", "contents": "Frank vectorcardiogram in common ventricle: correlation with anatomic findings. The influence on the Frank vectorcardiogram of anatomic features in common ventricle was analyzed by comparison of Frank vectorcardiograms in 51 patients who had common ventricle with those of 36 patients who had large ventricular septal defect, 14 of whom had levotransposition of the great arteries and 22 of whom had dextrotransposition. Frank vector loops in common ventricle differed from those in ventricular septal defect by the frequent occurrence of clockwise horizontal rotation, most common in patients with type C common ventricle (no outflow chamber). There was a statistically significant difference in the amplitude of early forces between patients with common ventricle and those with large ventricular septal defect. The degree of overlap of vectorcardiographic features between the two groups is sufficiently great that the vectorcardiogram has limited usefulness in establishing the diagnosis of common ventricle in any individual patient."}
{"id": "PMID:1099887", "title": "Left ventricular function in ischemic heart disease: assessment by noninvasive techniques.", "content": "1. Thirty-two patients (29 men and 3 women), admitted to a coronary-care unit with either acute coronary insufficiency or acute myocardial infarction, had their systolic time intervals and the a/E ratio of the apexcardiogram studied on days 1,2, and 7 of their hospital stay. 2. Only the LVETc and PEP/LVET were found to undergo any statistically significant change. Although all figures were in the abnormal range, they had no discriminative value in individuals. None of the other commonly accepted noninvasive  indices or left ventricular function, including the a/E ratio of the apexcardiogram, were found to be of assistance in the early distinction between acute coronary insufficiency and acute myocardial infarction.", "contents": "Left ventricular function in ischemic heart disease: assessment by noninvasive techniques. 1. Thirty-two patients (29 men and 3 women), admitted to a coronary-care unit with either acute coronary insufficiency or acute myocardial infarction, had their systolic time intervals and the a/E ratio of the apexcardiogram studied on days 1,2, and 7 of their hospital stay. 2. Only the LVETc and PEP/LVET were found to undergo any statistically significant change. Although all figures were in the abnormal range, they had no discriminative value in individuals. None of the other commonly accepted noninvasive  indices or left ventricular function, including the a/E ratio of the apexcardiogram, were found to be of assistance in the early distinction between acute coronary insufficiency and acute myocardial infarction."}
{"id": "PMID:1099888", "title": "The heart in myasthenia gravis.", "content": "The cardiac changes associated with myasthenia gravis have been reviewed and specific areas explored. There is no doubt concerning the involvement of the myocardium in this disease as indicated by clinical, ECG, vectorcardiographic, and autopsy data. The doubt lies in the precipitating factor for the pathology found. On the one hand the whole picture could be a direct result of the pathologic process of myasthenia gravis. On the other hand the patient with myasthenia gravis, during the natural history of the disease, encounters many iatrogenic and coincidental variables which could influence the nature of the clinical findings and autopsy data. One fact seems reasonably clear: The association of myocardial pathology with thymoma, especially malignant thymoma, is well established for the more severe form of the myocardial disease. Furthermore, the hypothesis that cardiac muscle antibodies give rise to such reactions is attractive, although not fully resolved. If such cases are excluded then there remain others where cardiac pathology could be due to other disease, primarily coronary heart disease, in the older group. In addition, the mode of death of some patients indicates that intercurrent respiratory problems could play a part. Most patients in this group do not seem to have cardiac abnormalities due to the pathologic process of myasthenia. There is in addition a small group where very striking cardiovascular findings suggestive of myocarditis are found. There is not necessarily a thymoma and no other specific etiologic factors can be found. These are rare cases and might still be related to disorders of immune mechanisms. In conclusion it can be stated that, although there is now a considerable body of evidence concerning the heart in myasthenia gravis, the implication of specific pathology for this neuromuscular disorder affecting the heart must be considered, in the traditional Scottish legal sense, as \"not proven.\"", "contents": "The heart in myasthenia gravis. The cardiac changes associated with myasthenia gravis have been reviewed and specific areas explored. There is no doubt concerning the involvement of the myocardium in this disease as indicated by clinical, ECG, vectorcardiographic, and autopsy data. The doubt lies in the precipitating factor for the pathology found. On the one hand the whole picture could be a direct result of the pathologic process of myasthenia gravis. On the other hand the patient with myasthenia gravis, during the natural history of the disease, encounters many iatrogenic and coincidental variables which could influence the nature of the clinical findings and autopsy data. One fact seems reasonably clear: The association of myocardial pathology with thymoma, especially malignant thymoma, is well established for the more severe form of the myocardial disease. Furthermore, the hypothesis that cardiac muscle antibodies give rise to such reactions is attractive, although not fully resolved. If such cases are excluded then there remain others where cardiac pathology could be due to other disease, primarily coronary heart disease, in the older group. In addition, the mode of death of some patients indicates that intercurrent respiratory problems could play a part. Most patients in this group do not seem to have cardiac abnormalities due to the pathologic process of myasthenia. There is in addition a small group where very striking cardiovascular findings suggestive of myocarditis are found. There is not necessarily a thymoma and no other specific etiologic factors can be found. These are rare cases and might still be related to disorders of immune mechanisms. In conclusion it can be stated that, although there is now a considerable body of evidence concerning the heart in myasthenia gravis, the implication of specific pathology for this neuromuscular disorder affecting the heart must be considered, in the traditional Scottish legal sense, as \"not proven.\""}
{"id": "PMID:1099889", "title": "Electrophysiology and pharmacology of cardiac arrhythmias. VIII. Cardiac effects of diphenylhydantoin. B.", "content": "DPH is highly effective against both atrial and ventricular arrhythmias resulting from digitalis toxicity and should be considered one of the primary antiarrhythmic drugs in this clinical situation. Clinical studies have indicated that DPH, for the most part, is effective against atrial arrhythmias and not markedly effective against ventricular arrhythmias associated with acute or chronic cardiac disease. For the latter ventricular arrhythmias, DPH is usually considered only after other antiarrhythmic drug therapy has failed. Studies on the mechanisms responsible for the antiarrhythmic effects of DPH are, as yet, inconclusive, There is good evidence that its actions differ from those of the commonly used drugs with local anesthetic effects. The extent to which it exerts direct effects on the heart which are therapeutically relevant remains to be demonstrated; further, there is solid evidence that its effects on the central nervous system may be of prime importance in its antiarrhythmic efficacy.", "contents": "Electrophysiology and pharmacology of cardiac arrhythmias. VIII. Cardiac effects of diphenylhydantoin. B. DPH is highly effective against both atrial and ventricular arrhythmias resulting from digitalis toxicity and should be considered one of the primary antiarrhythmic drugs in this clinical situation. Clinical studies have indicated that DPH, for the most part, is effective against atrial arrhythmias and not markedly effective against ventricular arrhythmias associated with acute or chronic cardiac disease. For the latter ventricular arrhythmias, DPH is usually considered only after other antiarrhythmic drug therapy has failed. Studies on the mechanisms responsible for the antiarrhythmic effects of DPH are, as yet, inconclusive, There is good evidence that its actions differ from those of the commonly used drugs with local anesthetic effects. The extent to which it exerts direct effects on the heart which are therapeutically relevant remains to be demonstrated; further, there is solid evidence that its effects on the central nervous system may be of prime importance in its antiarrhythmic efficacy."}
{"id": "PMID:1099894", "title": "L-Ascorbic acid and D-isoascorbic acid in a common cold survey.", "content": "In a survey of double-bind design, 3 groups of approximately 70 student volunteers took 1 g L-ascorbic acid, 1 g D-isoascorbic acid, or placebo tablets every day for 15 weeks and the incidence and duration of colds were recorded. The group taking D-isoascrobic acid suffered 34% fewer colds than the other two groups; the results were also analyzed in relation to the sex and smoking habits of the volunteers. The rationale for investigating D-isoascorbic acid, an isomer of L-ascorbic acid which has limited antiscorbutic activity, is discussed.", "contents": "L-Ascorbic acid and D-isoascorbic acid in a common cold survey. In a survey of double-bind design, 3 groups of approximately 70 student volunteers took 1 g L-ascorbic acid, 1 g D-isoascorbic acid, or placebo tablets every day for 15 weeks and the incidence and duration of colds were recorded. The group taking D-isoascrobic acid suffered 34% fewer colds than the other two groups; the results were also analyzed in relation to the sex and smoking habits of the volunteers. The rationale for investigating D-isoascorbic acid, an isomer of L-ascorbic acid which has limited antiscorbutic activity, is discussed."}
{"id": "PMID:1099895", "title": "Cardiorespiratory, hematological and physical performance responses of anemic subjects to iron treatment.", "content": "Twenty-nine adult iron-deficient anemis subjects (13 men and 16 women) with hemoglobin levels of 4.0 to 12.0 g/100 ml blood were divided into either an iron treatment or placebo group. Hematological, cardiorespiratory and performance data were collected before, during, and after treatment and compared with data from a control group of subjects (4 men and 6 women) from the same socioeconomic population. Hemoglobin levels for the iron treatment group improved from 7.7 to 12.4 g for the women and from 7.1 to 14.0 g for the men. Values for the control group were 13.9 g and 14.3 g for the women and men, respectively. The placebo group showed virtually no change over the 80-day period (8.1-8.4 g for women and 7.7-7.4 g for men). Peak exercise heart rates (5 min, 40-cm step test) were significantly reduced after treatment from 155 to 113 for the iron treatment men and 152 to 123 for the women compared with the placebo group which showed no changes. Values for the control group were 119 and 142 for the men and women, respectively. In response to the exercise test, no difference in oxygen consumption was found between the iron treatment and placebo group although 15% more O2 was delivered per pulse in the iron treatment group. Blood lactates were significantly highein the placebo than iron treatment group both at rest, 1.18 versus 0.64 mmole/liter, and 1 min after exercise, 5.30 versus 2.68 mmoles/liter. No changes in handgrip or shoulder adductor strength were observed following treatment. These results clearly support the concept that performance requiring high oxygen delivery is significantly affected by hemoglobin levels.", "contents": "Cardiorespiratory, hematological and physical performance responses of anemic subjects to iron treatment. Twenty-nine adult iron-deficient anemis subjects (13 men and 16 women) with hemoglobin levels of 4.0 to 12.0 g/100 ml blood were divided into either an iron treatment or placebo group. Hematological, cardiorespiratory and performance data were collected before, during, and after treatment and compared with data from a control group of subjects (4 men and 6 women) from the same socioeconomic population. Hemoglobin levels for the iron treatment group improved from 7.7 to 12.4 g for the women and from 7.1 to 14.0 g for the men. Values for the control group were 13.9 g and 14.3 g for the women and men, respectively. The placebo group showed virtually no change over the 80-day period (8.1-8.4 g for women and 7.7-7.4 g for men). Peak exercise heart rates (5 min, 40-cm step test) were significantly reduced after treatment from 155 to 113 for the iron treatment men and 152 to 123 for the women compared with the placebo group which showed no changes. Values for the control group were 119 and 142 for the men and women, respectively. In response to the exercise test, no difference in oxygen consumption was found between the iron treatment and placebo group although 15% more O2 was delivered per pulse in the iron treatment group. Blood lactates were significantly highein the placebo than iron treatment group both at rest, 1.18 versus 0.64 mmole/liter, and 1 min after exercise, 5.30 versus 2.68 mmoles/liter. No changes in handgrip or shoulder adductor strength were observed following treatment. These results clearly support the concept that performance requiring high oxygen delivery is significantly affected by hemoglobin levels."}
{"id": "PMID:1099896", "title": "Indirect fluorescent antibody technic for demonstration of serum antibody in dengue hemorrhagic fever cases.", "content": "One hundred eighty-three sera from two groups of children with dengue hemorrhagic fever and 37 control sera were examined for antibody against dengue virus by an indirect immunofluorescent method. When the reciprocal titer of 16 or higher was used as the diagnostic level, positive tests could be obtained in 80% of Group I and 100% of Group II sera after 3--6 days of fever. Positive tests were obtained in 100% of both groups after the first week of fever. There was no false-positive among the control sera. Preparation of the antigen is relatively easy, and the antigen may be stored for at least 3 months. The immunofluorescent method is rapid and simple, and is recommended for routine detection of serum antibody in dengue hemorrhagic fever.", "contents": "Indirect fluorescent antibody technic for demonstration of serum antibody in dengue hemorrhagic fever cases. One hundred eighty-three sera from two groups of children with dengue hemorrhagic fever and 37 control sera were examined for antibody against dengue virus by an indirect immunofluorescent method. When the reciprocal titer of 16 or higher was used as the diagnostic level, positive tests could be obtained in 80% of Group I and 100% of Group II sera after 3--6 days of fever. Positive tests were obtained in 100% of both groups after the first week of fever. There was no false-positive among the control sera. Preparation of the antigen is relatively easy, and the antigen may be stored for at least 3 months. The immunofluorescent method is rapid and simple, and is recommended for routine detection of serum antibody in dengue hemorrhagic fever."}
{"id": "PMID:1099897", "title": "Mucormycosis in a transplant recipient.", "content": "Mucormycosis classically occurs in patients who have uncontrolled diabetes who develop rhinocerebral disease. A fatal case of rhinocerebral infection caused by Rhizopus arrhizus in a 53-year-old man who had received a renal homograft three years previously is reported. Only five similar cases have been documented, all since 1970. Although direct smears of the purulent nasal exudate revealed the presence of numerous Gram-negative bacilli, later identified as Haemophilus influenzae, the diagnosis of mucormycosis was made by demonstrating the typical broad, nonseptate branched hyphae in the necrotic tissue obtained by surgical debridement of the paranasal sinuses. Culture of this material revealed growth of mold-like fungus which, upon direct microscopic examination, showed sporangiophores bearing spherical sporangia arising directly from a cluster of root-like structures of rhizoids. Despite the immediate institution of therapy with amphotericin B postoperatively, the patient died 48 hours later. Subsequently, the Rhizopus isolated was shown to be resistant to both amphotericin B and 5-fluorocytosine. The present case and two others stress the importance of an aggressive diagnostic approach to patients suspected of having mucormycosis, because the usual microbiologic technics are frequently, inexplicably, unsuccessful, and possibly even misleading in this disease.", "contents": "Mucormycosis in a transplant recipient. Mucormycosis classically occurs in patients who have uncontrolled diabetes who develop rhinocerebral disease. A fatal case of rhinocerebral infection caused by Rhizopus arrhizus in a 53-year-old man who had received a renal homograft three years previously is reported. Only five similar cases have been documented, all since 1970. Although direct smears of the purulent nasal exudate revealed the presence of numerous Gram-negative bacilli, later identified as Haemophilus influenzae, the diagnosis of mucormycosis was made by demonstrating the typical broad, nonseptate branched hyphae in the necrotic tissue obtained by surgical debridement of the paranasal sinuses. Culture of this material revealed growth of mold-like fungus which, upon direct microscopic examination, showed sporangiophores bearing spherical sporangia arising directly from a cluster of root-like structures of rhizoids. Despite the immediate institution of therapy with amphotericin B postoperatively, the patient died 48 hours later. Subsequently, the Rhizopus isolated was shown to be resistant to both amphotericin B and 5-fluorocytosine. The present case and two others stress the importance of an aggressive diagnostic approach to patients suspected of having mucormycosis, because the usual microbiologic technics are frequently, inexplicably, unsuccessful, and possibly even misleading in this disease."}
{"id": "PMID:1099898", "title": "Antacid vs placebo in hospitalized gastric ulcer patients: a controlled therapeutic study.", "content": "Antacids are widely accepted as agents that promote healing and relieve pain of gastric ulcer. Well-controlled studies designed to test this belief are few. 28 patients with endoscopically proven gastric ulcer were treated for 3 weeks in hospital, 13 receiving a liquid placebo and 15 an antacid. All were followed by endoscopy to complete healing or until surgery was performed. 10 patients healed satisfactorily in the placebo group and 11 in the antacid group. All patients were free of pain during their hospitalization. From this study it is concluded that in gastric ulcer patients hospitalized for 3 weeks, the rate of healing of the ulcer and the relief of pain is not influenced by treatment with a standard antacid preparation.", "contents": "Antacid vs placebo in hospitalized gastric ulcer patients: a controlled therapeutic study. Antacids are widely accepted as agents that promote healing and relieve pain of gastric ulcer. Well-controlled studies designed to test this belief are few. 28 patients with endoscopically proven gastric ulcer were treated for 3 weeks in hospital, 13 receiving a liquid placebo and 15 an antacid. All were followed by endoscopy to complete healing or until surgery was performed. 10 patients healed satisfactorily in the placebo group and 11 in the antacid group. All patients were free of pain during their hospitalization. From this study it is concluded that in gastric ulcer patients hospitalized for 3 weeks, the rate of healing of the ulcer and the relief of pain is not influenced by treatment with a standard antacid preparation."}
{"id": "PMID:1099899", "title": "Adaptive changes in vitamin B12 absorption in celiac disease and after proximal small-bowel resection in man.", "content": "Resection of the proximal small bowel is known to cause mucosal hyperplasia and enhanced absorption in the ileum of experimental animals, but similar adaptive changes had not previously been studied in man. Since intrinsic-factor-bound vitamin B12 (IF-B12) absorption is confined to the ileum, as an index of ileal adaptation, we measured whole-body IF-58 Co B12 absorption in 24 control subjects, in 4 patients after proximal small-bowel resection, and in 9 patients with adult celiac disease (where mucosal damage is often limited to the proximal intestine and spares the ileum). Control subjects absorbed 20.4% (+/- 1 SD 6.2%) of the administered 5-mug dose of vitamin B12, while the corresponding 7-day retention values in patients with proximal resection (mean 42.3%; range 32-61%) and in 2 of the 9 celiac patients (44.1% and 54%, respectively), were above the normal range. The increased vitamin B12 absorption in these patients suggest that functional adaptation also occurs in the ileum in man. The results also illustrate the application of a newly developed whole-body counting technique to study vitamin B12 absorption in man.", "contents": "Adaptive changes in vitamin B12 absorption in celiac disease and after proximal small-bowel resection in man. Resection of the proximal small bowel is known to cause mucosal hyperplasia and enhanced absorption in the ileum of experimental animals, but similar adaptive changes had not previously been studied in man. Since intrinsic-factor-bound vitamin B12 (IF-B12) absorption is confined to the ileum, as an index of ileal adaptation, we measured whole-body IF-58 Co B12 absorption in 24 control subjects, in 4 patients after proximal small-bowel resection, and in 9 patients with adult celiac disease (where mucosal damage is often limited to the proximal intestine and spares the ileum). Control subjects absorbed 20.4% (+/- 1 SD 6.2%) of the administered 5-mug dose of vitamin B12, while the corresponding 7-day retention values in patients with proximal resection (mean 42.3%; range 32-61%) and in 2 of the 9 celiac patients (44.1% and 54%, respectively), were above the normal range. The increased vitamin B12 absorption in these patients suggest that functional adaptation also occurs in the ileum in man. The results also illustrate the application of a newly developed whole-body counting technique to study vitamin B12 absorption in man."}
{"id": "PMID:1099900", "title": "An outbreak of salmonellosis propagated by person-to-person transmission on an Indian reservation.", "content": "Between December 21, 1969, and April 14, 1970, 44 symptomatic cases of Salmonella typhimurium gastroenteritis occurred among the approximately 2500 Sioux Indians of the Lake Traverse Reservation in South Dakota. Twenty-five cases were confirmed by positive stool culture. All 19 cases not confirmed by culture had diarrhea and were epidemiologically associated with the culture-proven cases. Fourteen of these were discovered during the course of the investigation and had not been cultured previously. Twelve cases were hospital-acquired and 32 community acquired. Both the nosocomial and community-acquired infections occurred randomly during the 17-week span of the epidemic. Despite extensive investigation, no common exposure was discovered. The hospital-acquired infections all occurred in patients who shared a room or nursing personnel with patients who had active disease, or were born of a woman with active disease at the time of parturition. Twenty-nine of the 32 community acquired cases were linked to each other by person-to-person contact. This epidemic is the first documented outbreak of non-institutional salmonellosis propagated by person-to-person transmission.", "contents": "An outbreak of salmonellosis propagated by person-to-person transmission on an Indian reservation. Between December 21, 1969, and April 14, 1970, 44 symptomatic cases of Salmonella typhimurium gastroenteritis occurred among the approximately 2500 Sioux Indians of the Lake Traverse Reservation in South Dakota. Twenty-five cases were confirmed by positive stool culture. All 19 cases not confirmed by culture had diarrhea and were epidemiologically associated with the culture-proven cases. Fourteen of these were discovered during the course of the investigation and had not been cultured previously. Twelve cases were hospital-acquired and 32 community acquired. Both the nosocomial and community-acquired infections occurred randomly during the 17-week span of the epidemic. Despite extensive investigation, no common exposure was discovered. The hospital-acquired infections all occurred in patients who shared a room or nursing personnel with patients who had active disease, or were born of a woman with active disease at the time of parturition. Twenty-nine of the 32 community acquired cases were linked to each other by person-to-person contact. This epidemic is the first documented outbreak of non-institutional salmonellosis propagated by person-to-person transmission."}
{"id": "PMID:1099901", "title": "Normal chemoreceptor function in obesity before and after ileal bypass surgery to force weight reduction.", "content": "Ventilatory responses to progressive isocapnic hypoxia and rebreathing of carbon dioxide in oxygen were determined in four obese women before and approximately 1 year after ileal bypass surgery to force weight reduction. None of the patients was hypoventilating and all had normal pulmonary function tests. The ventilatory responses to hypoxia were normal before surgery and were not effected by weight reduction. The ventilatory responses to hypercapnia did not change in slope but a shift of the carbon dioxide response line toward a lower arterial carbon dioxide tension occurred in two subjects after weight reduction. We conclude that obesity per se does not necessarily cause loss of hypoxic ventilatory drive.", "contents": "Normal chemoreceptor function in obesity before and after ileal bypass surgery to force weight reduction. Ventilatory responses to progressive isocapnic hypoxia and rebreathing of carbon dioxide in oxygen were determined in four obese women before and approximately 1 year after ileal bypass surgery to force weight reduction. None of the patients was hypoventilating and all had normal pulmonary function tests. The ventilatory responses to hypoxia were normal before surgery and were not effected by weight reduction. The ventilatory responses to hypercapnia did not change in slope but a shift of the carbon dioxide response line toward a lower arterial carbon dioxide tension occurred in two subjects after weight reduction. We conclude that obesity per se does not necessarily cause loss of hypoxic ventilatory drive."}
{"id": "PMID:1099902", "title": "Leukemic reticuloendotheliosis. A study of the origin of the malignant cell.", "content": "A highly pure preparation of neoplastic cells from the spleen of a patient with leukemic reticuloendotheliosis was studied for function, membrane characteristics and glucose metabolism. Glass adherence and phagocytosis of small particles (latex and carbon black) were demonstrated with phase contrast microscopy. Staphylocidal activity was similar to that of normal monocytes. Immunofluorescent assays revealed nonspecific uptake of antiserums to immunoglobulins G (IgG), M (IgM), A (IgA) and kappa and kappa and lambda light chains. Rosette assays indicated the presence of receptors for IgG on the surface of all cells but no receptors for complement (C3) or sheep red blood cells. Glucose metabolic studies revealed a pattern that differed from that of normal monocytes or lymphocytes with intermediate values for glycolysis, low hexose monophosphate shunt activity and high Krebs cycle activity. Increments in tritiated (3H)-thymidine uptake and glucose metabolism in response to phytohemagglutinin stimulation were minimal (5 per cent of normal lymphocyte values) and no response was noted with pokeweed mitogen stimulation. These findings suggest that the leukemic reticuloendotheliosis cell most closely resembles cells of the monocyte-histiocyte series.", "contents": "Leukemic reticuloendotheliosis. A study of the origin of the malignant cell. A highly pure preparation of neoplastic cells from the spleen of a patient with leukemic reticuloendotheliosis was studied for function, membrane characteristics and glucose metabolism. Glass adherence and phagocytosis of small particles (latex and carbon black) were demonstrated with phase contrast microscopy. Staphylocidal activity was similar to that of normal monocytes. Immunofluorescent assays revealed nonspecific uptake of antiserums to immunoglobulins G (IgG), M (IgM), A (IgA) and kappa and kappa and lambda light chains. Rosette assays indicated the presence of receptors for IgG on the surface of all cells but no receptors for complement (C3) or sheep red blood cells. Glucose metabolic studies revealed a pattern that differed from that of normal monocytes or lymphocytes with intermediate values for glycolysis, low hexose monophosphate shunt activity and high Krebs cycle activity. Increments in tritiated (3H)-thymidine uptake and glucose metabolism in response to phytohemagglutinin stimulation were minimal (5 per cent of normal lymphocyte values) and no response was noted with pokeweed mitogen stimulation. These findings suggest that the leukemic reticuloendotheliosis cell most closely resembles cells of the monocyte-histiocyte series."}
{"id": "PMID:1099906", "title": "Increased and prolonged LH-RH/FSH-RH activity of synthetic (D-Ala6, Des-Gly-NH210)-LH-RH-ethylamide in normal women.", "content": "A synthetic LH-RH analogue (D-Ala6, Des-Gly-NH210)-LH-RH ethylamide exhibited an increased and prolonged LH-RH/FSH-RH activity in normal women. The integrated amounts of LH and FSH levels for this LH-RH analogue were about nine and five times greater than for the same doses of synthetic LH-RH. It is expected that this synthetic LH-RH analogue might yield more positive results than with LH-RH when used in infertility problems.", "contents": "Increased and prolonged LH-RH/FSH-RH activity of synthetic (D-Ala6, Des-Gly-NH210)-LH-RH-ethylamide in normal women. A synthetic LH-RH analogue (D-Ala6, Des-Gly-NH210)-LH-RH ethylamide exhibited an increased and prolonged LH-RH/FSH-RH activity in normal women. The integrated amounts of LH and FSH levels for this LH-RH analogue were about nine and five times greater than for the same doses of synthetic LH-RH. It is expected that this synthetic LH-RH analogue might yield more positive results than with LH-RH when used in infertility problems."}
{"id": "PMID:1099910", "title": "Change in cell type of human choroidal malignant melanoma in tissue culture.", "content": "In five cases choroidal malignant melanoma spontaneously converted from epitheloid to spindle cell and vice versa. The cultured epitheloid cells divided more frequently and grew faster than the spindle cells. Cells from one tumor were cloned and developed into a cell line.", "contents": "Change in cell type of human choroidal malignant melanoma in tissue culture. In five cases choroidal malignant melanoma spontaneously converted from epitheloid to spindle cell and vice versa. The cultured epitheloid cells divided more frequently and grew faster than the spindle cells. Cells from one tumor were cloned and developed into a cell line."}
{"id": "PMID:1099911", "title": "Uveitis and reticulum cell sarcoma of brain with bilateral neoplastic seeding of vitreous without retinal or uveal involvement.", "content": "A 65-year-old woman had been observed for more than four years with bilateral chronic nongranulomatous uveitis and vitreous clouding of unknown cause. Her death was from reticulum cell sarcoma of the brain (microglioma). Both eyes were obtained post mortem. Histopathologic examination revealed malignant cell in the vitreous of both eyes, but no other tumor was demonstrable in the ocular tissues. Reticulum cell sarcoma should be suspected in middle-aged or older persons with chronic unilateral or bilateral uveitis of unknown cause who develop cerebral manifestations during the course of the disease.", "contents": "Uveitis and reticulum cell sarcoma of brain with bilateral neoplastic seeding of vitreous without retinal or uveal involvement. A 65-year-old woman had been observed for more than four years with bilateral chronic nongranulomatous uveitis and vitreous clouding of unknown cause. Her death was from reticulum cell sarcoma of the brain (microglioma). Both eyes were obtained post mortem. Histopathologic examination revealed malignant cell in the vitreous of both eyes, but no other tumor was demonstrable in the ocular tissues. Reticulum cell sarcoma should be suspected in middle-aged or older persons with chronic unilateral or bilateral uveitis of unknown cause who develop cerebral manifestations during the course of the disease."}
{"id": "PMID:1099912", "title": "The past 25 years of surgery.", "content": "The past 25 years have seen an explosion in interest and progress in the diagnosis and treatment of vitreoretinal diseases, especially retinal detachments. This progress has paid high dividends in the prevention of blindness and the clinical care of patients. The major advances during this period are the development of a binocular stereoscopic indirect ophthalmoscope combined with scleral depression, the introduction of a scleral buckling procedure to close retinal breaks, the development of instruments capable of producing controlled focal irritation in the ocular fundus, and the advent of direct surgical removal of the vitreous.", "contents": "The past 25 years of surgery. The past 25 years have seen an explosion in interest and progress in the diagnosis and treatment of vitreoretinal diseases, especially retinal detachments. This progress has paid high dividends in the prevention of blindness and the clinical care of patients. The major advances during this period are the development of a binocular stereoscopic indirect ophthalmoscope combined with scleral depression, the introduction of a scleral buckling procedure to close retinal breaks, the development of instruments capable of producing controlled focal irritation in the ocular fundus, and the advent of direct surgical removal of the vitreous."}
{"id": "PMID:1099913", "title": "Unintentional filtration after cataract surgery.", "content": "The eyes of an 81-year-old man were obtained three and ten days after intracapsular extractions had been made through limbal incisions under limbal-based flaps of conjunctiva and Tenon's capsule. The wounds were closed with 7-0 chromic collagen. Before death from bronchopneumonia, the patient developed bilateral filtrations in association with violent coughing spells. In both specimens, the healing processes of the conjunctiva and Tenon's capsule were advanced, confining the escaping aqueous humor to the subepithelial tissues of the flap. In contrast, the stromal wounds were still inert in accordance with our earlier observations that these wounds are precariously weak during the first two weeks after surgery. In cataract surgery, flaps including both conjunctiva and Tenon's capsule should be prepared and closed to support the slower healing stromal wound. Prevention of filtrations is not a simple matter of using many sutures. This patient's eyes demonstrated that improperly placed or too tightly tied sutures may be directly responsible for leaking wounds.", "contents": "Unintentional filtration after cataract surgery. The eyes of an 81-year-old man were obtained three and ten days after intracapsular extractions had been made through limbal incisions under limbal-based flaps of conjunctiva and Tenon's capsule. The wounds were closed with 7-0 chromic collagen. Before death from bronchopneumonia, the patient developed bilateral filtrations in association with violent coughing spells. In both specimens, the healing processes of the conjunctiva and Tenon's capsule were advanced, confining the escaping aqueous humor to the subepithelial tissues of the flap. In contrast, the stromal wounds were still inert in accordance with our earlier observations that these wounds are precariously weak during the first two weeks after surgery. In cataract surgery, flaps including both conjunctiva and Tenon's capsule should be prepared and closed to support the slower healing stromal wound. Prevention of filtrations is not a simple matter of using many sutures. This patient's eyes demonstrated that improperly placed or too tightly tied sutures may be directly responsible for leaking wounds."}
{"id": "PMID:1099914", "title": "Immunologic study of nonsyphilitic interstitial keratitis with vestibuloauditory symptoms.", "content": "We studied four patients with the clinical entity of nonsyphilitic interstitial keratitis and vestibuloauditory symptoms to determine whether immunologic abnormalities play a role in its pathophysiology. There was no evidence of alteration in nonspecific cell-mediated or humoral immunologic status of any of the patients studied. Three of the four patients had W-17, a histocompatibility antigen that occurs in 3 to 10% of the normal population. All four patients had increased cell-mediated immunity against normal allogenic corneal antigens compared to normal control subjects. There seems to be no apparent generalized alteration of the immune system in this syndrome.", "contents": "Immunologic study of nonsyphilitic interstitial keratitis with vestibuloauditory symptoms. We studied four patients with the clinical entity of nonsyphilitic interstitial keratitis and vestibuloauditory symptoms to determine whether immunologic abnormalities play a role in its pathophysiology. There was no evidence of alteration in nonspecific cell-mediated or humoral immunologic status of any of the patients studied. Three of the four patients had W-17, a histocompatibility antigen that occurs in 3 to 10% of the normal population. All four patients had increased cell-mediated immunity against normal allogenic corneal antigens compared to normal control subjects. There seems to be no apparent generalized alteration of the immune system in this syndrome."}
{"id": "PMID:1099915", "title": "Clinical evaluation of adenine arabinoside and idoxuridine in the treatment of ocular herpes simplex.", "content": "A Double-blind clinical study comparing idoxuridine (IDU) with adenine arabinoside (ara-A) in treating 54 routine herpetic ulcers, and an open ara-A therapy study of 58 herpetic ulcers in patients intolerant of or resistant to IDU, was carried out over a four-year period. There was no significant difference in healing time between IDU-treated eyes (11.5 days) and ara-A-treated eyes (12.4 days) in the double-blind study but there were four moderate to marked adverse reactions to IDU and only two mild reactions to ara-A. In the open-drug study 21 patients who were intolerant of IDU because of allergy or toxicity and 37 patients who had ulcers resistant to or deteriorating on IDU therapy used ara-A up to 192 days without any adverse reaction. Mean healing time was 10.6 days for 49 of 57 patients in the efficacy analysis. Eight patients developed trophic ulcers that responded to soft lens therapy and one was dropped from the study because his initial disease could not be distinguished from severe IDU-induced keratitis.", "contents": "Clinical evaluation of adenine arabinoside and idoxuridine in the treatment of ocular herpes simplex. A Double-blind clinical study comparing idoxuridine (IDU) with adenine arabinoside (ara-A) in treating 54 routine herpetic ulcers, and an open ara-A therapy study of 58 herpetic ulcers in patients intolerant of or resistant to IDU, was carried out over a four-year period. There was no significant difference in healing time between IDU-treated eyes (11.5 days) and ara-A-treated eyes (12.4 days) in the double-blind study but there were four moderate to marked adverse reactions to IDU and only two mild reactions to ara-A. In the open-drug study 21 patients who were intolerant of IDU because of allergy or toxicity and 37 patients who had ulcers resistant to or deteriorating on IDU therapy used ara-A up to 192 days without any adverse reaction. Mean healing time was 10.6 days for 49 of 57 patients in the efficacy analysis. Eight patients developed trophic ulcers that responded to soft lens therapy and one was dropped from the study because his initial disease could not be distinguished from severe IDU-induced keratitis."}
{"id": "PMID:1099916", "title": "Bacterial endophthalmitis following suture removal after penetrating keratoplasty.", "content": "Of three cases of bacterial endophthalmitis occurring after removal of corneal sutures following penetrating keratoplasty, two were associated with wound dehiscence and one was not. Factors that may predispose to wound dehiscence and subsequent infection are: use of fine suture material, corticosteroids, and chronic topical antibiotics; early removal of corneal sutures; lack of adherence to strict asepsis prior to and after suture removal; and open suture tracts that may be kept open by incarceration of vitreous strands. Late corneal wound dehiscence may occur with subsequent development of endophthalmitis as long as three days after suture removal. The use of topical antibiotics before and after suture removal may decrease the potential for infection. In addition, early recognition of intraocular infection by the patient and the surgeon for immediate institution of antimicrobial and anti-inflammatory therapy may preserve useful vision.", "contents": "Bacterial endophthalmitis following suture removal after penetrating keratoplasty. Of three cases of bacterial endophthalmitis occurring after removal of corneal sutures following penetrating keratoplasty, two were associated with wound dehiscence and one was not. Factors that may predispose to wound dehiscence and subsequent infection are: use of fine suture material, corticosteroids, and chronic topical antibiotics; early removal of corneal sutures; lack of adherence to strict asepsis prior to and after suture removal; and open suture tracts that may be kept open by incarceration of vitreous strands. Late corneal wound dehiscence may occur with subsequent development of endophthalmitis as long as three days after suture removal. The use of topical antibiotics before and after suture removal may decrease the potential for infection. In addition, early recognition of intraocular infection by the patient and the surgeon for immediate institution of antimicrobial and anti-inflammatory therapy may preserve useful vision."}
{"id": "PMID:1099917", "title": "Corneal holder.", "content": "As a result of the widespread use of M-K (McCarey-Kaufman) medium preserved corneas, as well as other methods of preserving corneas in a viable state, I developed a corneal holder to facilitate the lamellar dissection of previously excised whole human corneas. Consisting of a moderately heavy base, cutting pedestal, scleral rim-sealing sleeve, and retaining ring, the corneal holder is economically manufactured, simple to use, and easily sterilized. Its weight and construction allow unassisted dissection of a lamellar graft of any size.", "contents": "Corneal holder. As a result of the widespread use of M-K (McCarey-Kaufman) medium preserved corneas, as well as other methods of preserving corneas in a viable state, I developed a corneal holder to facilitate the lamellar dissection of previously excised whole human corneas. Consisting of a moderately heavy base, cutting pedestal, scleral rim-sealing sleeve, and retaining ring, the corneal holder is economically manufactured, simple to use, and easily sterilized. Its weight and construction allow unassisted dissection of a lamellar graft of any size."}
{"id": "PMID:1099919", "title": "Research on variation in dental occlusion. A \"state of the art\" workshop conducted by the Craniofacial Anomalies Program, the National Institute of Dental Research.", "content": "The following report on assessing research on variation in dental occlusion was based in part on a workshop conducted by the National Institute of Dental Research at the initiative of Richard L. Christiansen, Chief, Craniofacial Anomalies Program. The meeting was planned and developed by Robert J. Isaacson, Chairman, Touro M. Graber, Richard A. Riedel, and Richard L. Christiansen. This report is designed to provide a review of the achievements, directions, and needs of research concerning variations in dental occlusion. The workshop was held at the National Institutes of Health, Bethesda, Maryland, on Nov 22 and 23, 1972. The material presented by the workshop participants has been summarized and incorporated in this report. The subject of research related to the field of malocclusion was discussed in an article published in the American Journal of Orthodontics in January, 1971.", "contents": "Research on variation in dental occlusion. A \"state of the art\" workshop conducted by the Craniofacial Anomalies Program, the National Institute of Dental Research. The following report on assessing research on variation in dental occlusion was based in part on a workshop conducted by the National Institute of Dental Research at the initiative of Richard L. Christiansen, Chief, Craniofacial Anomalies Program. The meeting was planned and developed by Robert J. Isaacson, Chairman, Touro M. Graber, Richard A. Riedel, and Richard L. Christiansen. This report is designed to provide a review of the achievements, directions, and needs of research concerning variations in dental occlusion. The workshop was held at the National Institutes of Health, Bethesda, Maryland, on Nov 22 and 23, 1972. The material presented by the workshop participants has been summarized and incorporated in this report. The subject of research related to the field of malocclusion was discussed in an article published in the American Journal of Orthodontics in January, 1971."}
{"id": "PMID:1099923", "title": "Interstitial therapy suing non-absorbable (Ir192 nylon ribbon) and absrobable (I125 \"Vicryl\") suturing techniques.", "content": "Permanent and removable interstitial implantation techniques using absorbable and unabsorbable sutures are described. Most of these techniques can be performed in the clinic easily and quickly with basic instruments: needle holder, needle book, and hemoclips. Specifically, Ir192 (74-4 day half life, 300-610 keV, and 6.0 cm. hvl in tissue) nylon ribbon and I125 (60 day half life, 27-35 keV,  and 2.0 cm. hvl in tissue) Vicryl sutures are described. A major advantage of the I125 over the Ir192 see (other than the fact that it can be permanently implanted and needs less radiation protection) is that the patient does not remain highly radioactive for as long a period due to hte extremely low I125 energy and may be allowed to leave the hospital. Both nuclides have the advantage of a long shelf life, making their use practical and economically feasible.", "contents": "Interstitial therapy suing non-absorbable (Ir192 nylon ribbon) and absrobable (I125 \"Vicryl\") suturing techniques. Permanent and removable interstitial implantation techniques using absorbable and unabsorbable sutures are described. Most of these techniques can be performed in the clinic easily and quickly with basic instruments: needle holder, needle book, and hemoclips. Specifically, Ir192 (74-4 day half life, 300-610 keV, and 6.0 cm. hvl in tissue) nylon ribbon and I125 (60 day half life, 27-35 keV,  and 2.0 cm. hvl in tissue) Vicryl sutures are described. A major advantage of the I125 over the Ir192 see (other than the fact that it can be permanently implanted and needs less radiation protection) is that the patient does not remain highly radioactive for as long a period due to hte extremely low I125 energy and may be allowed to leave the hospital. Both nuclides have the advantage of a long shelf life, making their use practical and economically feasible."}
{"id": "PMID:1099929", "title": "[Postoperative astigmatism after penetrating corneal grafts (author's transl)].", "content": "232 cases of penetrating grafts from 1961-1972 have been studied. 141 (60%) were evaluated for visual acuity and corneal astigmatism. Since the introduction of the running suture (1966) postoperative complications have markedly decreased, visual acuity is in the average good and even very good. Patients operated for keratokonus however show higher postoperative astigmatism than patients operatedfor other corneal diseases.", "contents": "[Postoperative astigmatism after penetrating corneal grafts (author's transl)]. 232 cases of penetrating grafts from 1961-1972 have been studied. 141 (60%) were evaluated for visual acuity and corneal astigmatism. Since the introduction of the running suture (1966) postoperative complications have markedly decreased, visual acuity is in the average good and even very good. Patients operated for keratokonus however show higher postoperative astigmatism than patients operatedfor other corneal diseases."}
{"id": "PMID:1099930", "title": "[Clinical picture and morphology of orbital rhabdomyosarcomas in children].", "content": "The clinical course of orbital rhabdomyosarcoma is described in seven male children. A survey of the literature on the subject is given. Particular attention is paid to certain diagnostic problems of this most frequent orbital malignant primary in childhood. An adequate combination therapy which results in cure in more than half of the patients should be employed provided that an exact morphological diagnosis is made; this still causes often great difficulty. Electron microscopy is of great value in distinguishing this type of tumor from other malignancies, including the so-called reticulosarcoma. In comparison with the case material of other authors, there was a relatively high rate of late deaths in our patients.", "contents": "[Clinical picture and morphology of orbital rhabdomyosarcomas in children]. The clinical course of orbital rhabdomyosarcoma is described in seven male children. A survey of the literature on the subject is given. Particular attention is paid to certain diagnostic problems of this most frequent orbital malignant primary in childhood. An adequate combination therapy which results in cure in more than half of the patients should be employed provided that an exact morphological diagnosis is made; this still causes often great difficulty. Electron microscopy is of great value in distinguishing this type of tumor from other malignancies, including the so-called reticulosarcoma. In comparison with the case material of other authors, there was a relatively high rate of late deaths in our patients."}
{"id": "PMID:1099931", "title": "Immunological investigations of patients with transplanted cornea.", "content": "Authors examined the role of the cellular immunity by the method (I) of lymphoblastic transformation in 26 keratoplasty cases. Only in one case did they observe significant blast transformation, in this case the keratoplasty was necessary after injury with lime. In the rest of the cases no positive result were ever obtained. They investigated the cellular immunoreactivity of 10 patients waiting for keratoplasty and 10 cataractous patients against cornea antigen by means (II) of the Leucocyte Migration Test (LMT). The change of the cellular immuno-reactivity against cornea antigen was examined on the 1st, 4th, 7th, 14th, 21st, and 28th days after keratoplasty. It could be established that after keratoplasty the LMT became positive (inhibition or stimulation of migration). The results of the LMT investigations obtained on keratoplasty patients by cornea antigen may be of some help for the understanding of the events in the postoperative phase and sometimes also may eventually require immunosuppressive therapy.", "contents": "Immunological investigations of patients with transplanted cornea. Authors examined the role of the cellular immunity by the method (I) of lymphoblastic transformation in 26 keratoplasty cases. Only in one case did they observe significant blast transformation, in this case the keratoplasty was necessary after injury with lime. In the rest of the cases no positive result were ever obtained. They investigated the cellular immunoreactivity of 10 patients waiting for keratoplasty and 10 cataractous patients against cornea antigen by means (II) of the Leucocyte Migration Test (LMT). The change of the cellular immuno-reactivity against cornea antigen was examined on the 1st, 4th, 7th, 14th, 21st, and 28th days after keratoplasty. It could be established that after keratoplasty the LMT became positive (inhibition or stimulation of migration). The results of the LMT investigations obtained on keratoplasty patients by cornea antigen may be of some help for the understanding of the events in the postoperative phase and sometimes also may eventually require immunosuppressive therapy."}
{"id": "PMID:1099932", "title": "Immunological investigations on patients with transplanted cornea: the role of tissue antigens in keratoplasty.", "content": "After briefly describing the conceptions of the HL-A antigens and of histocompatibility, the authors give an account of their own retrospective examinations in connection with keratoplasty. Transplantations between identical groups of the ABO-system have a greater probability of ending successfully, if at least 2 HL-A antigens of donor and recipient are identical; this being chiefly important when grafting is carried out into unfavourable, vascularized surroundings.", "contents": "Immunological investigations on patients with transplanted cornea: the role of tissue antigens in keratoplasty. After briefly describing the conceptions of the HL-A antigens and of histocompatibility, the authors give an account of their own retrospective examinations in connection with keratoplasty. Transplantations between identical groups of the ABO-system have a greater probability of ending successfully, if at least 2 HL-A antigens of donor and recipient are identical; this being chiefly important when grafting is carried out into unfavourable, vascularized surroundings."}
{"id": "PMID:1099941", "title": "[Karyometric studies of the pancreas and adrenal glands of the albino-rat fed an unbalanced glucose diet or a glucose diet supplemented with thiamine].", "content": "Karyometric measurements in rats which for 30 days had received a glucose diet or a glucose diet supplemented with thiamine showed the following decrease in nuclear size in contrast to no treated controls. (see article) In both experimental groups there was a 30% loss of weight during the course of the experiment. The different decreases in nuclear volume in the examined organs cannot be explained only by a relative state of hunger. The very large decrease in activity in the fasciculated zone of the adrenal cortex is explained as an adaptation mechanism favouring glucose oxidation by insulin, and the relative increase in activity in the adrenal medulla as an adaptation mechanism to accomplish an elevated lipolysis.", "contents": "[Karyometric studies of the pancreas and adrenal glands of the albino-rat fed an unbalanced glucose diet or a glucose diet supplemented with thiamine]. Karyometric measurements in rats which for 30 days had received a glucose diet or a glucose diet supplemented with thiamine showed the following decrease in nuclear size in contrast to no treated controls. (see article) In both experimental groups there was a 30% loss of weight during the course of the experiment. The different decreases in nuclear volume in the examined organs cannot be explained only by a relative state of hunger. The very large decrease in activity in the fasciculated zone of the adrenal cortex is explained as an adaptation mechanism favouring glucose oxidation by insulin, and the relative increase in activity in the adrenal medulla as an adaptation mechanism to accomplish an elevated lipolysis."}
{"id": "PMID:1099944", "title": "Relationship among skin tests, bronchial challenge and serology in house dust and Candida albicans allergic asthma.", "content": "One hundred and ninety-six asthmatic patients were examined by inhalation challenge using house dust and candida albicans; an early, dual or late bronchial response was induced where IgE, C3 and C4 were elevated. In some of late responses by candida inhalation there was a decrease or almost no increase in IgE and a fall in C3 and C4. There was a considerable correlation between skin tests and bronchial responses. An induced bronchial response, early, dual or late, was identified as Type I, While candida-induced late bronchial responses were Type I and Type III. There is some question whether Type I plus Type III mechanism may be involved in the dual bronchial response provoked with candida.", "contents": "Relationship among skin tests, bronchial challenge and serology in house dust and Candida albicans allergic asthma. One hundred and ninety-six asthmatic patients were examined by inhalation challenge using house dust and candida albicans; an early, dual or late bronchial response was induced where IgE, C3 and C4 were elevated. In some of late responses by candida inhalation there was a decrease or almost no increase in IgE and a fall in C3 and C4. There was a considerable correlation between skin tests and bronchial responses. An induced bronchial response, early, dual or late, was identified as Type I, While candida-induced late bronchial responses were Type I and Type III. There is some question whether Type I plus Type III mechanism may be involved in the dual bronchial response provoked with candida."}
{"id": "PMID:1099945", "title": "Ru-Tuss in the symptomatic treatment of allergic rhinitis.", "content": "In the treatment of allergic rhinitis with a placebo only one symptom, nasal itching, was reduced significantly. Where Ru-Tuss tablets were used, all symptoms improved and statistically significant reduction of  symptoms were obtained for lacrimation, nasal itching, sneezing, itching of the mouth, itching of the pharynx, rhinorrhea and the condition of the nasal mucosa. Ridit analysis was used to evaluate differences between the Ru-Tuss Tablet and the placebo treated groups.", "contents": "Ru-Tuss in the symptomatic treatment of allergic rhinitis. In the treatment of allergic rhinitis with a placebo only one symptom, nasal itching, was reduced significantly. Where Ru-Tuss tablets were used, all symptoms improved and statistically significant reduction of  symptoms were obtained for lacrimation, nasal itching, sneezing, itching of the mouth, itching of the pharynx, rhinorrhea and the condition of the nasal mucosa. Ridit analysis was used to evaluate differences between the Ru-Tuss Tablet and the placebo treated groups."}
{"id": "PMID:1099946", "title": "Isolation and identification of mycobacteria from porcine tissues: a three-year summary.", "content": "Mycobacteria were isolated from 1,591 (78%) of 2,036 porcine tissues submitted to Veterinary Services Laboratories over a 3-year period (July 1, 1971, to June 30, 1974). The isolates were identified by biochemical and serologic tests. Of the 1,547 Mycobacterium avium isolates, 452 were serotype 1, 728 were serotype 2, 60 were serotyped 4, 110 were serotype 8, and 51 were serotyped 10; 36 isolates represented 11 other serotypes; 65 isolates shared antigens with more than one serotype; and 45 isolates, untypabley serologic methods, were identified by pathogenicity in chickens as M avium. Mycobacterium bovis was isolated from 15 tissues; Mycobacterium fortuitum, from 3; and Mycobacterium paratuberculosis, Mycobacterium scrofulaceum, and Mycobacterium xenopi were each isolated from single specimens.", "contents": "Isolation and identification of mycobacteria from porcine tissues: a three-year summary. Mycobacteria were isolated from 1,591 (78%) of 2,036 porcine tissues submitted to Veterinary Services Laboratories over a 3-year period (July 1, 1971, to June 30, 1974). The isolates were identified by biochemical and serologic tests. Of the 1,547 Mycobacterium avium isolates, 452 were serotype 1, 728 were serotype 2, 60 were serotyped 4, 110 were serotype 8, and 51 were serotyped 10; 36 isolates represented 11 other serotypes; 65 isolates shared antigens with more than one serotype; and 45 isolates, untypabley serologic methods, were identified by pathogenicity in chickens as M avium. Mycobacterium bovis was isolated from 15 tissues; Mycobacterium fortuitum, from 3; and Mycobacterium paratuberculosis, Mycobacterium scrofulaceum, and Mycobacterium xenopi were each isolated from single specimens."}
{"id": "PMID:1099947", "title": "Tissue-washing tank for histologic preparations.", "content": "A small tank made of plexiglass was designed to wash tissues for histologic preparations. The tank was divided into 16 compartments in 2 rows to wash 16 tissue samples at one time. The compartments in the same row alternately intercommunicated by rows of holes in the upper or lower halves. The arrangement of holes at 2 water levels in the partition appeared to create silent water waves, making the tank more efficient for washing tissues.", "contents": "Tissue-washing tank for histologic preparations. A small tank made of plexiglass was designed to wash tissues for histologic preparations. The tank was divided into 16 compartments in 2 rows to wash 16 tissue samples at one time. The compartments in the same row alternately intercommunicated by rows of holes in the upper or lower halves. The arrangement of holes at 2 water levels in the partition appeared to create silent water waves, making the tank more efficient for washing tissues."}
{"id": "PMID:1099948", "title": "A comparative study of atropine sulfate and isoproterenol hydrochloride in chronic bronchitis.", "content": "Fifteen subjects with chronic bronchitis were treated with inhaled atropine sulfate, isoproterenol hydrochloride, or placebo in a double blind crossover trial. Each drug was inhaled 4 times per day for 3 weeks. Baseline forced expiratory flows, specific airway conductance, airway resistnace, and functional residual capacity were measured weekly. These measurements were repeated 15 min and 60 min after laboratory inhalation of each agent, using a separate double blind protocol. No significant differences were observed among the mean baseline function studies during the 3 treatment periods. There were no significant differences in sputum production, sputum thickness, dyspnea, or mouth dryness among the 3 treatment periods. Side effects were mild; isoproterenol treatment was associated with an increased frequency of palpitations, and atropine treatment was associated with difficulty in voiding. Significant increases in lung function were apparent in the measurements made 15 min and 60 min after inhalation of atropine or isoproterenol. Mean values of flow and specific airway conductance increased, and mean values of airway resistance and functional residual capacity decreased after inhalation of either drug. There was no significant difference between the effects of atropine and isoproterenol on lung function, although both differed significantly from the effects of placebo (P less than 0.001). Inhaled atropine sulfate was as effective a bronchodilator as inhaled isoproterenol hydrochloride, and was well tolerated during the 3-week trial of daily therapy.", "contents": "A comparative study of atropine sulfate and isoproterenol hydrochloride in chronic bronchitis. Fifteen subjects with chronic bronchitis were treated with inhaled atropine sulfate, isoproterenol hydrochloride, or placebo in a double blind crossover trial. Each drug was inhaled 4 times per day for 3 weeks. Baseline forced expiratory flows, specific airway conductance, airway resistnace, and functional residual capacity were measured weekly. These measurements were repeated 15 min and 60 min after laboratory inhalation of each agent, using a separate double blind protocol. No significant differences were observed among the mean baseline function studies during the 3 treatment periods. There were no significant differences in sputum production, sputum thickness, dyspnea, or mouth dryness among the 3 treatment periods. Side effects were mild; isoproterenol treatment was associated with an increased frequency of palpitations, and atropine treatment was associated with difficulty in voiding. Significant increases in lung function were apparent in the measurements made 15 min and 60 min after inhalation of atropine or isoproterenol. Mean values of flow and specific airway conductance increased, and mean values of airway resistance and functional residual capacity decreased after inhalation of either drug. There was no significant difference between the effects of atropine and isoproterenol on lung function, although both differed significantly from the effects of placebo (P less than 0.001). Inhaled atropine sulfate was as effective a bronchodilator as inhaled isoproterenol hydrochloride, and was well tolerated during the 3-week trial of daily therapy."}
{"id": "PMID:1099949", "title": "Effects of smoked marijuana in experimentally induced asthma.", "content": "After experimental induction of acute bronchospasm in 8 subjects with clinically stable bronchial asthma, effects of 500 mg of smoked marijuana (2.0 per cent delta9-tetrahydrocannabinol) on specific airway conductance and thoracic gas volume were compared with those of 500 mg of smoked placebo marijuana (0.0 per cent delta9-tetrahydrocannabinol), 0.25 ml of aerosolized saline, and 0.25 ml of aerosolized isoproterenol (1,250 mug). Bronchospasm was induced on 4 separate occasions, by inhalation of methacholine and, on four other occasions, by exercise on a bicycle ergometer or treadmill. Methacholine and exercise caused average decreases in specific airway conductance of 40 to 55 per cent and 30 to 39 per cent, respectively, and average increases in thoracic gas volume of 35 to 43 per cent and 25 to 35 per cent, respectively. After methacholine-induced bronchospasm, placebo marijuana and saline inhalation produced minimal changes in specific airway conductance and thoracic gas volume, whereas 2.0 per cent marijuana and isoproterenol each caused a prompt correction of the bronchospasm and associated hyperinflation. After exercise-induced bronchospasm, placebo marijuana and saline were followed by gradual recovery during 30 to 60 min, whereas 2.0 per cent marijuana and isoproterenol caused an immediate reversal of exercise-induced asthma and hyperinflation.", "contents": "Effects of smoked marijuana in experimentally induced asthma. After experimental induction of acute bronchospasm in 8 subjects with clinically stable bronchial asthma, effects of 500 mg of smoked marijuana (2.0 per cent delta9-tetrahydrocannabinol) on specific airway conductance and thoracic gas volume were compared with those of 500 mg of smoked placebo marijuana (0.0 per cent delta9-tetrahydrocannabinol), 0.25 ml of aerosolized saline, and 0.25 ml of aerosolized isoproterenol (1,250 mug). Bronchospasm was induced on 4 separate occasions, by inhalation of methacholine and, on four other occasions, by exercise on a bicycle ergometer or treadmill. Methacholine and exercise caused average decreases in specific airway conductance of 40 to 55 per cent and 30 to 39 per cent, respectively, and average increases in thoracic gas volume of 35 to 43 per cent and 25 to 35 per cent, respectively. After methacholine-induced bronchospasm, placebo marijuana and saline inhalation produced minimal changes in specific airway conductance and thoracic gas volume, whereas 2.0 per cent marijuana and isoproterenol each caused a prompt correction of the bronchospasm and associated hyperinflation. After exercise-induced bronchospasm, placebo marijuana and saline were followed by gradual recovery during 30 to 60 min, whereas 2.0 per cent marijuana and isoproterenol caused an immediate reversal of exercise-induced asthma and hyperinflation."}
{"id": "PMID:1099952", "title": "Highlights in the development of arterial surgery.", "content": "It is concluded that progress in medicine has been maintained at such a rapid pace that the delivery system has had difficulty in keeping up. This has been excellently demonstrated by the progress in the category of vascular surgery. Achievements have placed added demands on available trained personnel, materiel, and most importantly, the financial resources of every community and the government fulfilled, the problem will continue to be a vexing one and will not be easy to solve. The success of our scientific efforts will continue to be costly.", "contents": "Highlights in the development of arterial surgery. It is concluded that progress in medicine has been maintained at such a rapid pace that the delivery system has had difficulty in keeping up. This has been excellently demonstrated by the progress in the category of vascular surgery. Achievements have placed added demands on available trained personnel, materiel, and most importantly, the financial resources of every community and the government fulfilled, the problem will continue to be a vexing one and will not be easy to solve. The success of our scientific efforts will continue to be costly."}
{"id": "PMID:1099953", "title": "Repair of hiatal hernia: description and long-term results of a technique.", "content": "A modification of the surgical approach for the correction of hiatal hernia is presented. The technique involves use of the phrenoesophageal ligament as a structure of support for the repair, along with the strength of the deep submucosal sutures through the stomach and esophagus. An additional modification is the buffering of the sutures with teflon felt pledgets to prevent tearing through of the sutures. Long-term follow-up was obtained in 30 patients. There was one recurrence. All patients remain symptom-free to date.", "contents": "Repair of hiatal hernia: description and long-term results of a technique. A modification of the surgical approach for the correction of hiatal hernia is presented. The technique involves use of the phrenoesophageal ligament as a structure of support for the repair, along with the strength of the deep submucosal sutures through the stomach and esophagus. An additional modification is the buffering of the sutures with teflon felt pledgets to prevent tearing through of the sutures. Long-term follow-up was obtained in 30 patients. There was one recurrence. All patients remain symptom-free to date."}
{"id": "PMID:1099957", "title": "T- and B-cell interactions in autoimmune syndromes.", "content": "We have reviewed briefly some of the individual capabilities of T and B cells and how these can be modified by interactions between the two cell lines. Evidence that T cells have their own distinct receptors for antigens, yet under certain circumstances may bind IgM or IgG produced by B cells, has been particularly emphasized. The probability that B cells, in turn, may bind the antigen receptor of T-cell origin reflects the balanced nature of an intricate communication system in which interactions between antigens, antigen receptors, and binding sites for these receptors all serve to modulate the integrated functioning of T and B cells. It is suggested that this communication system is disturbed in patients with rheumatoid arthritis; specifically, it is proposed that defective feedback activity of IgG-antigen complexes on activated T cells may exist in some patients and could result in unchecked and harmful T-cell activity in the joint. Therapeutic implications of this idea are mentioned.", "contents": "T- and B-cell interactions in autoimmune syndromes. We have reviewed briefly some of the individual capabilities of T and B cells and how these can be modified by interactions between the two cell lines. Evidence that T cells have their own distinct receptors for antigens, yet under certain circumstances may bind IgM or IgG produced by B cells, has been particularly emphasized. The probability that B cells, in turn, may bind the antigen receptor of T-cell origin reflects the balanced nature of an intricate communication system in which interactions between antigens, antigen receptors, and binding sites for these receptors all serve to modulate the integrated functioning of T and B cells. It is suggested that this communication system is disturbed in patients with rheumatoid arthritis; specifically, it is proposed that defective feedback activity of IgG-antigen complexes on activated T cells may exist in some patients and could result in unchecked and harmful T-cell activity in the joint. Therapeutic implications of this idea are mentioned."}
{"id": "PMID:1099958", "title": "Lymphokines in rheumatoid synovitis.", "content": "In antigen-induced experimental arthritis of rabbits, a macrophage migration inhibitory factor was released from the inflamed synovial tissues. A migration inhibitory factor, blastogenic factor, and B-cell-stimulating factor were also found in human rheumatoid synovial fluids and culture supernatants of rheumatoid tissue explants. Joint fluids from patients with inflammatory conditions other than RA sometimes also displayed these activities. OA fluids were usually inactive. At present, little is known of the origin or role in vivo of the lymphokine-like activities observed in the joints of rheumatoid patients. In related experiments, injection of lymphokine-rich antigen-free lymphocyte supernatants into normal rabbit knee joints produced a synovitis characterized by lining layer hyperplasia and infiltration of the sublining layer by macrophages. The lymphocytic and plasmacytic components seen in active antigen-induced synovitis were absent. It seems likely that some of the changes observed in active chronic synovitis are mediated by soluble factors of the lymphokine variety.", "contents": "Lymphokines in rheumatoid synovitis. In antigen-induced experimental arthritis of rabbits, a macrophage migration inhibitory factor was released from the inflamed synovial tissues. A migration inhibitory factor, blastogenic factor, and B-cell-stimulating factor were also found in human rheumatoid synovial fluids and culture supernatants of rheumatoid tissue explants. Joint fluids from patients with inflammatory conditions other than RA sometimes also displayed these activities. OA fluids were usually inactive. At present, little is known of the origin or role in vivo of the lymphokine-like activities observed in the joints of rheumatoid patients. In related experiments, injection of lymphokine-rich antigen-free lymphocyte supernatants into normal rabbit knee joints produced a synovitis characterized by lining layer hyperplasia and infiltration of the sublining layer by macrophages. The lymphocytic and plasmacytic components seen in active antigen-induced synovitis were absent. It seems likely that some of the changes observed in active chronic synovitis are mediated by soluble factors of the lymphokine variety."}
{"id": "PMID:1099959", "title": "Physiologic and pharmacologic alterations of rat leukocyte chemotaxis (Cx) in vivo.", "content": "The method of adoptively transferring 51Cr-labeled rat leukocytes iv to isologous recipients was used to quantitate the extravascular accumulation of specific cell types at the site of inflammation induced by local injection of various phlogistic agents. Experiments were designed to determine whether cellular accumulation could be modified at the level of the chemotactic factor, by serum components, or by alteration of the responding cell itself. The results indicated a selective attraction of mononuclear cells to the local injection site of BCG and of neutrophils to the injection site of aggregated but not monometric gamma-globulin. Thus, leukocytic accumulation was found to be dependent upon the local generation of specific reactants that were particular to the agent employed. Leukocytic accumulation could also be modified by serum factors. Cellular accumulation was inhibited when leukocytes were exposed to serum that contained phagocytosable particles or after phagocytosis in vitro prior to adoptive transfer. Chemotaxis of lymphocytes could be induced by their preincubation with sera from adjuvant arthritic animals. These observations were confirmed by in vitro studies and by the finding that 6 days after adjuvant injection , lymphocytes but not mononuclear cells accumulated at the noninjected extremity. In the final series of experiments, it was shown that BCG immunization was capable of inducing a unique population of peritoneal mononuclear cells that after adoptive transfer had an enhanced capacity to remain in the circulation, which, in turn, resulted in a functional increase in their accumulation at an inflammatory reaction site. In conclusion, these studies indicated that the chemotactic activity of adoptively transferred cells can be modified by changes in the chemotactic stimuli, can be enhanced or depressed by serum factors, and is a function of the physiologic capability of the cell population employed.", "contents": "Physiologic and pharmacologic alterations of rat leukocyte chemotaxis (Cx) in vivo. The method of adoptively transferring 51Cr-labeled rat leukocytes iv to isologous recipients was used to quantitate the extravascular accumulation of specific cell types at the site of inflammation induced by local injection of various phlogistic agents. Experiments were designed to determine whether cellular accumulation could be modified at the level of the chemotactic factor, by serum components, or by alteration of the responding cell itself. The results indicated a selective attraction of mononuclear cells to the local injection site of BCG and of neutrophils to the injection site of aggregated but not monometric gamma-globulin. Thus, leukocytic accumulation was found to be dependent upon the local generation of specific reactants that were particular to the agent employed. Leukocytic accumulation could also be modified by serum factors. Cellular accumulation was inhibited when leukocytes were exposed to serum that contained phagocytosable particles or after phagocytosis in vitro prior to adoptive transfer. Chemotaxis of lymphocytes could be induced by their preincubation with sera from adjuvant arthritic animals. These observations were confirmed by in vitro studies and by the finding that 6 days after adjuvant injection , lymphocytes but not mononuclear cells accumulated at the noninjected extremity. In the final series of experiments, it was shown that BCG immunization was capable of inducing a unique population of peritoneal mononuclear cells that after adoptive transfer had an enhanced capacity to remain in the circulation, which, in turn, resulted in a functional increase in their accumulation at an inflammatory reaction site. In conclusion, these studies indicated that the chemotactic activity of adoptively transferred cells can be modified by changes in the chemotactic stimuli, can be enhanced or depressed by serum factors, and is a function of the physiologic capability of the cell population employed."}
{"id": "PMID:1099964", "title": "Activation of the complement and properdin systems in rheumatoid arthritis.", "content": "The classic (C1, C4, and C2) and properdin factors (D, C3b, and B) of complement generate C3 convertases that are capable of cleaving C3 and subsequently activating C5-C9. Both C1 and factor D are serine esterases, and both convertases undergo decay and regeneration. In seropositive rheumatoid arthritis, where intraarticular activation of the classic early components (C1, C4, and C2) by immunoglobulin complexes appears to predominate, findings of relative depressions in synovial fluid levels of factor B indicate recruitment of the amplification loop (D, C3b, and B), and relative declines in properdin levels suggest activation of the properdin pathway as well. Quantitative analysis of the complement system in disease states requires several different approaches: measurement of function and antigenic concentration to assess the functional integrity of the protein; determination of component metabolism to appreciate the relative contributions of hypercatabolism and hyper- or hyposynthesis to the plasma level; and for compartmentalized disease, measurement of the component in the appropriate biologic fluid and determination of local tissue synthesis.", "contents": "Activation of the complement and properdin systems in rheumatoid arthritis. The classic (C1, C4, and C2) and properdin factors (D, C3b, and B) of complement generate C3 convertases that are capable of cleaving C3 and subsequently activating C5-C9. Both C1 and factor D are serine esterases, and both convertases undergo decay and regeneration. In seropositive rheumatoid arthritis, where intraarticular activation of the classic early components (C1, C4, and C2) by immunoglobulin complexes appears to predominate, findings of relative depressions in synovial fluid levels of factor B indicate recruitment of the amplification loop (D, C3b, and B), and relative declines in properdin levels suggest activation of the properdin pathway as well. Quantitative analysis of the complement system in disease states requires several different approaches: measurement of function and antigenic concentration to assess the functional integrity of the protein; determination of component metabolism to appreciate the relative contributions of hypercatabolism and hyper- or hyposynthesis to the plasma level; and for compartmentalized disease, measurement of the component in the appropriate biologic fluid and determination of local tissue synthesis."}
{"id": "PMID:1099975", "title": "[Sequential analysis of effectiveness of diazepam, a tranquilizer, on stress in swine (author's transl)].", "content": "To study the effectiveness of diazepam, a tranquilizer, in alleviating the problems resulting from the mixing and moving of weaned piglets from one piggery to another, two procedures, one of short-duration transport and the other of long-duration transport, are used in a double blind clinical trial. Both followed mixing the pigs, but before they entered the new piggery. Results from both groups of pigs (treated and control) are sequentially analyzed. Measurements used are growth rate and feed conversion curves during the first 18-20 days of the prefattening period. Diazepam (I mg/kg IM) reduces feed conversion when used to alleviate the effects of the long journey, but increases feed conversion after the short journey when compared to controls injected with a placebo. Worsening effects seem related to increased aggressiveness in animals treated and transported. These results indicate that a better understanding of the action mechanisms of psychotropic drugs is necessary, and they should be used with caution in husbandry.", "contents": "[Sequential analysis of effectiveness of diazepam, a tranquilizer, on stress in swine (author's transl)]. To study the effectiveness of diazepam, a tranquilizer, in alleviating the problems resulting from the mixing and moving of weaned piglets from one piggery to another, two procedures, one of short-duration transport and the other of long-duration transport, are used in a double blind clinical trial. Both followed mixing the pigs, but before they entered the new piggery. Results from both groups of pigs (treated and control) are sequentially analyzed. Measurements used are growth rate and feed conversion curves during the first 18-20 days of the prefattening period. Diazepam (I mg/kg IM) reduces feed conversion when used to alleviate the effects of the long journey, but increases feed conversion after the short journey when compared to controls injected with a placebo. Worsening effects seem related to increased aggressiveness in animals treated and transported. These results indicate that a better understanding of the action mechanisms of psychotropic drugs is necessary, and they should be used with caution in husbandry."}
{"id": "PMID:1099969", "title": "Effect of ovine hydatid cyst fluid on the cardiovascular and respiratory systems in sheep.", "content": "Rupture of the hydatid cyst in man brings about mild to severe toxic reactions including death. The present study was undertaken to investigate some of the responses resulting from administration of the ovine hydatid fluid to the sheep, which, like man, is an intermediate host of the Echinococcus granulosus. In 50 sodium pentobarbital-anesthetized sheep, the arterial blood pressure (A.B.P.), central venous pressure (C.V.P.), respiration and electrocardiogram were recorded. Intraveonus administration of 5-10 ml hydatid fluid brought about moderate to severe fall in A.B.P. and rapid respiration with or without transient apnea or permanent repiratory cessation in 80 percent of the animals. Fifty percent of the sheep died of circulatory and respiratory failure after the first injection of the hydatid fluid. Boiled hydatid fluid did not lose its potency to evoke the above responses. Pretreatment of the aminals with atropine sulfate, 0,5 mg/kg subcutaneously, did not block the reactions. Administration of the antihistamine chlorpheniramine, 4 mg/kg intravenously, caused partial prevention of the reactions in 6 out of 10 responsive sheep. The cardiovascular and respiratory responses to ovine hydatid fluid may be due to antigen-antibody reactions or some toxic component of the fluid.", "contents": "Effect of ovine hydatid cyst fluid on the cardiovascular and respiratory systems in sheep. Rupture of the hydatid cyst in man brings about mild to severe toxic reactions including death. The present study was undertaken to investigate some of the responses resulting from administration of the ovine hydatid fluid to the sheep, which, like man, is an intermediate host of the Echinococcus granulosus. In 50 sodium pentobarbital-anesthetized sheep, the arterial blood pressure (A.B.P.), central venous pressure (C.V.P.), respiration and electrocardiogram were recorded. Intraveonus administration of 5-10 ml hydatid fluid brought about moderate to severe fall in A.B.P. and rapid respiration with or without transient apnea or permanent repiratory cessation in 80 percent of the animals. Fifty percent of the sheep died of circulatory and respiratory failure after the first injection of the hydatid fluid. Boiled hydatid fluid did not lose its potency to evoke the above responses. Pretreatment of the aminals with atropine sulfate, 0,5 mg/kg subcutaneously, did not block the reactions. Administration of the antihistamine chlorpheniramine, 4 mg/kg intravenously, caused partial prevention of the reactions in 6 out of 10 responsive sheep. The cardiovascular and respiratory responses to ovine hydatid fluid may be due to antigen-antibody reactions or some toxic component of the fluid."}
{"id": "PMID:1099981", "title": "Diffusion of trimethoprim and sulfamethoxazole from susceptibility disks into agar medium.", "content": "The standard practice of using a single susceptibility disk for the antimicrobial combination Septra (trimethoprim/sulfamethoxazole) has been further justified by a direct measurement of the diffusion rates of each compound through agar medium. [(14)C]trimethoprim and [(35)S]sulfamethoxazole, singly and in combination, were applied to blank susceptibility disks which were incubated on 4-inch (10.16-cm) agar plates (Mueller-Hinton medium) at 37 C. The migration of each compound from the disk and diffusion through agar were measured with time by determining the radioactivity in concentric zones extending from the origin. The two compounds diffuse with similar rates, maintaining approximately a 1:20 concentration ratio which is approximately the ratio of trimethoprim to sulfamethoxazole observed in plasma during treatment. The diffusion rate is independent of the presence of the other compound; greater than 95% of the radioactivity is transferred from the disk to the agar in 24 h.", "contents": "Diffusion of trimethoprim and sulfamethoxazole from susceptibility disks into agar medium. The standard practice of using a single susceptibility disk for the antimicrobial combination Septra (trimethoprim/sulfamethoxazole) has been further justified by a direct measurement of the diffusion rates of each compound through agar medium. [(14)C]trimethoprim and [(35)S]sulfamethoxazole, singly and in combination, were applied to blank susceptibility disks which were incubated on 4-inch (10.16-cm) agar plates (Mueller-Hinton medium) at 37 C. The migration of each compound from the disk and diffusion through agar were measured with time by determining the radioactivity in concentric zones extending from the origin. The two compounds diffuse with similar rates, maintaining approximately a 1:20 concentration ratio which is approximately the ratio of trimethoprim to sulfamethoxazole observed in plasma during treatment. The diffusion rate is independent of the presence of the other compound; greater than 95% of the radioactivity is transferred from the disk to the agar in 24 h."}
{"id": "PMID:1099982", "title": "Activity of minocycline against Acinetobacter calcoaceticus var. anitratus (syn. Herellea vaginicola) and Serratia marcescens.", "content": "The activity of minocycline and tetracycline against 23 isolates of Acinetobacter calcoaceticus var. anitratus (syn. Herellea vaginicola) and 178 strains of Serratia marcescens was determined by disk and microdilution methods. The results indicate that minocycline is highly active against this species of Acinetobacter, all but one strain being inhibited by 0.007 mug of the antibiotic per ml. Tetracycline was also active, though to a lesser degree, against A. calcoaceticus. Of the 178 strains of S. marcescens tested, only seven (3.9%) had a minimum inhibitory concentration of 2 mug or less of minocycline per ml. Tetracycline was less active than minocycline against S. marcescens; with 2 mug of tetracycline per ml, only 2 of 152 (1.3%) strains were inhibited. At concentrations of 8 and 16 mug of minocycline per ml, which can be achieved in the urine with the usual doses, 44.9 and 63.5% of S. marcescens strains were inhibited, which implies its possible usefulness for the therapy of urinary tract infection due to this organism.", "contents": "Activity of minocycline against Acinetobacter calcoaceticus var. anitratus (syn. Herellea vaginicola) and Serratia marcescens. The activity of minocycline and tetracycline against 23 isolates of Acinetobacter calcoaceticus var. anitratus (syn. Herellea vaginicola) and 178 strains of Serratia marcescens was determined by disk and microdilution methods. The results indicate that minocycline is highly active against this species of Acinetobacter, all but one strain being inhibited by 0.007 mug of the antibiotic per ml. Tetracycline was also active, though to a lesser degree, against A. calcoaceticus. Of the 178 strains of S. marcescens tested, only seven (3.9%) had a minimum inhibitory concentration of 2 mug or less of minocycline per ml. Tetracycline was less active than minocycline against S. marcescens; with 2 mug of tetracycline per ml, only 2 of 152 (1.3%) strains were inhibited. At concentrations of 8 and 16 mug of minocycline per ml, which can be achieved in the urine with the usual doses, 44.9 and 63.5% of S. marcescens strains were inhibited, which implies its possible usefulness for the therapy of urinary tract infection due to this organism."}
{"id": "PMID:1099978", "title": "Clinical and laboratory considerations in metabolic bone disease.", "content": "An overview of the common types of metabolic bone disease is described. When the disease is present in pure form, diagnosis is not difficult. When mixed disease is present, as may be the case, the pathophysiology involved must be clearly understood for accurate diagnosis and treatment.", "contents": "Clinical and laboratory considerations in metabolic bone disease. An overview of the common types of metabolic bone disease is described. When the disease is present in pure form, diagnosis is not difficult. When mixed disease is present, as may be the case, the pathophysiology involved must be clearly understood for accurate diagnosis and treatment."}
{"id": "PMID:1099983", "title": "Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF13: purification and characterization of the immunity protein.", "content": "Cloacin DF13 inhibits protein synthesis both in vivo and in vitro by inactivation of ribosomes. In this paper we describe the purification, from purified bacteriocinogenic plasmid DF13 (Clo DF13)-harboring minicells of Escherichia coli, of an acidic protein (molecular weight about 10,000) that prevents the inhibition of in vitro protein synthesis by cloacin DF13. It turned out that this protein is one of three Clo DF13 plasmid-specific polypeptides that are synthesized by the noninduced Clo DF13 plasmid in minicells. Furthermore, we observed that, after induction with mitomycin C, the amount of immunity protein is only slightly increased, whereas the cloacin DF13 synthesis is increased about 40 times. These results imply that the genes that code for immunity protein and cloacin DF13 are not located in the same regulatory unit. Under noninduced conditions, apparently an excess of immunity protein is synthesized, because this amount of immunity protein is even sufficient to neutralize the strongly increased amount of cloacin after induction, since growth of induced cloacinogenic cells is continued.", "contents": "Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF13: purification and characterization of the immunity protein. Cloacin DF13 inhibits protein synthesis both in vivo and in vitro by inactivation of ribosomes. In this paper we describe the purification, from purified bacteriocinogenic plasmid DF13 (Clo DF13)-harboring minicells of Escherichia coli, of an acidic protein (molecular weight about 10,000) that prevents the inhibition of in vitro protein synthesis by cloacin DF13. It turned out that this protein is one of three Clo DF13 plasmid-specific polypeptides that are synthesized by the noninduced Clo DF13 plasmid in minicells. Furthermore, we observed that, after induction with mitomycin C, the amount of immunity protein is only slightly increased, whereas the cloacin DF13 synthesis is increased about 40 times. These results imply that the genes that code for immunity protein and cloacin DF13 are not located in the same regulatory unit. Under noninduced conditions, apparently an excess of immunity protein is synthesized, because this amount of immunity protein is even sufficient to neutralize the strongly increased amount of cloacin after induction, since growth of induced cloacinogenic cells is continued."}
{"id": "PMID:1099979", "title": "Immunologic aspects of myasthenia gravis.", "content": "Clinical and pathologic features of myasthenia gravis suggest a possible autoimmune etiology. Myasthenia is found in association with other putative autoimmune diseases and autoantibodies including anti-muscle antibodies. There is also in vitro evidence for cell-mediated immunity to muscle in blood and thymic cells as well as a suggestion of antigenic disparity between blood and thymic cells. In addition, two experimental animal models are under investigation. It seems likely that the immune system plays a role in the pathogenesis, but what that role is and how it relates to neuromuscular block is not clear.", "contents": "Immunologic aspects of myasthenia gravis. Clinical and pathologic features of myasthenia gravis suggest a possible autoimmune etiology. Myasthenia is found in association with other putative autoimmune diseases and autoantibodies including anti-muscle antibodies. There is also in vitro evidence for cell-mediated immunity to muscle in blood and thymic cells as well as a suggestion of antigenic disparity between blood and thymic cells. In addition, two experimental animal models are under investigation. It seems likely that the immune system plays a role in the pathogenesis, but what that role is and how it relates to neuromuscular block is not clear."}
{"id": "PMID:1099984", "title": "Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF16: action of the immunity protein in vivo and in vitro.", "content": "The Clo DF13 plasmid-specific immunity protein is able to prevent the inhibitory effect of cloacin DF13 on in vitro protein synthesis. We have shown, by gel filtration, that direct binding of the Clo DF13 immunity protein to cloacin occurs in vitro. This cloacin DF13-immunity protein complex is rather stable, and the cloacin present in the complex is no longer able to cause inhibition of in vitro protein synthesis. The binding of immunity protein to cloacin DF13 is rather specific because the Clo DF13 immunity protein does not bind to in vitro inactive cloacin and binds very poorly to the closely related bacteriocin colicin E3. Furthermore, we present data which strongly suggest that in vitro at least a fourfold excess of immunity protein is required to ensure that every cloacin molecule is inactivated by cloacin-immunity protein complex formation. Only a fraction (an about equimolar amount) of the immunity protein molecules, however, actually binds to cloacin DF13. The existence of an immunity protein-cloacin complex in vivo was concluded from the observation that cloacin, purified by chromatography on diethyl-(2-hydroxypropyl)-aminoethyl Sephadex in the absence of urea, contains an about equimolar amount of a second protein which comigrates with immunity protein on sodium dodecyl sulfate-polyacrylamide and urea-polyacrylamide gels. In an in vitro protein-synthesizing system, this component appeared to behave identical to the Clo DF13 immunity protein. The purified immunity protein-containing cloacin was at least 80 times less active in inhibiting in vitro protein synthesis, compared to cloacin, free of immunity protein. These data imply that few, if any, cloacin DF13 molecules are present in cloacinogenic cells as active, free cloacin molecules.", "contents": "Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF16: action of the immunity protein in vivo and in vitro. The Clo DF13 plasmid-specific immunity protein is able to prevent the inhibitory effect of cloacin DF13 on in vitro protein synthesis. We have shown, by gel filtration, that direct binding of the Clo DF13 immunity protein to cloacin occurs in vitro. This cloacin DF13-immunity protein complex is rather stable, and the cloacin present in the complex is no longer able to cause inhibition of in vitro protein synthesis. The binding of immunity protein to cloacin DF13 is rather specific because the Clo DF13 immunity protein does not bind to in vitro inactive cloacin and binds very poorly to the closely related bacteriocin colicin E3. Furthermore, we present data which strongly suggest that in vitro at least a fourfold excess of immunity protein is required to ensure that every cloacin molecule is inactivated by cloacin-immunity protein complex formation. Only a fraction (an about equimolar amount) of the immunity protein molecules, however, actually binds to cloacin DF13. The existence of an immunity protein-cloacin complex in vivo was concluded from the observation that cloacin, purified by chromatography on diethyl-(2-hydroxypropyl)-aminoethyl Sephadex in the absence of urea, contains an about equimolar amount of a second protein which comigrates with immunity protein on sodium dodecyl sulfate-polyacrylamide and urea-polyacrylamide gels. In an in vitro protein-synthesizing system, this component appeared to behave identical to the Clo DF13 immunity protein. The purified immunity protein-containing cloacin was at least 80 times less active in inhibiting in vitro protein synthesis, compared to cloacin, free of immunity protein. These data imply that few, if any, cloacin DF13 molecules are present in cloacinogenic cells as active, free cloacin molecules."}
{"id": "PMID:1099985", "title": "Survival of frozen mycoplasmas.", "content": "Cooling to -70 C killed a higher percentage of Acholeplasma laidlawii and Mycoplasma mycoides var. capri cells than cooling to -20 C. However, to preserve cell viability for prolonged periods storage at -70 C was much more preferable. The percentage of cells surviving freezing could be increased by increasing the initial cell concentration or by the addition of dimethyl sulfoxide or glycerol as cryoprotective agents. In the presence of 1.5 M of any one of these agents survival rates of up to 100% could be obtained. The optimal cooling rates for maximal survival of A. laidlawii under the experimental conditions tested were 11 C/min for cooling to -20 C and about 15 C/min for cooling to -70 C. Increasing the warming rate during thawing from 0.6 to 67 C/min increased survival by 3 log. Oleic acid enrichment of A. laidlawii membrane lipids, or reduction in the cholesterol content of M. mycoides var. capri membranes, increased the percentage of organisms surviving freezing. Hence, the composition of membrane lipids appears to have a marked influence on the susceptibility of mycoplasmas to freezing injury.", "contents": "Survival of frozen mycoplasmas. Cooling to -70 C killed a higher percentage of Acholeplasma laidlawii and Mycoplasma mycoides var. capri cells than cooling to -20 C. However, to preserve cell viability for prolonged periods storage at -70 C was much more preferable. The percentage of cells surviving freezing could be increased by increasing the initial cell concentration or by the addition of dimethyl sulfoxide or glycerol as cryoprotective agents. In the presence of 1.5 M of any one of these agents survival rates of up to 100% could be obtained. The optimal cooling rates for maximal survival of A. laidlawii under the experimental conditions tested were 11 C/min for cooling to -20 C and about 15 C/min for cooling to -70 C. Increasing the warming rate during thawing from 0.6 to 67 C/min increased survival by 3 log. Oleic acid enrichment of A. laidlawii membrane lipids, or reduction in the cholesterol content of M. mycoides var. capri membranes, increased the percentage of organisms surviving freezing. Hence, the composition of membrane lipids appears to have a marked influence on the susceptibility of mycoplasmas to freezing injury."}
{"id": "PMID:1099986", "title": "Comparative effects of anesthetics on the viability and integrity of Escherichia coli ML30.", "content": "Cells of Escherichia coli ML30 in a mineral salts medium were exposed to dichlorodifluoromethane (f-12), cyclopropane, halothane, or Ethrane at concentrations of 1.25, 0.2, 0.04, and 0.008 X saturation for times up to 1,200 min, and at temperatures in the range of 2 to 37 C. When any of these anesthetics were applied for 300 min at 1.25 X saturation, a substantial decrease in number of survivors occurred. Halothane was most bactericidal, cyclopropane and Ethrane were moderately bactericidal, and t-12 was least bactericidal. At saturation values of less than 1.0, none of the four anesthetics had an appreciable effect on viability of E. coli. Greatest increases in cell permeability occurred when anesthetics were used at saturation values of 1.25, and permeability changes generally decreased as the concentrations of the chemicals were reduced. In many instances, anesthetics in the vapor state caused significant increases in cell permeability but little or no loss of viability. This indicated that a close relationship did not exist between loss of viability and increased permeability. All four anesthetics caused E. coli to lose substantial and similar amounts of compounds absorbing at 260 nm. Release of compounds absorbing at 260 nm generally increased as the saturation value of a given chemical was increased. Halothane, Ethrane, and cyclopropane but not f-12 caused lysis of E. coli ML300. Considering all results, E. coli ML30 was damaged more by halothane or cyclopropane than by f-12 or Ethrane. When f-12 was applied at a saturation value of 1.25, the bactericidal effect on E. coli was much greater at 37 or 22 C than at 12 or 2 C.", "contents": "Comparative effects of anesthetics on the viability and integrity of Escherichia coli ML30. Cells of Escherichia coli ML30 in a mineral salts medium were exposed to dichlorodifluoromethane (f-12), cyclopropane, halothane, or Ethrane at concentrations of 1.25, 0.2, 0.04, and 0.008 X saturation for times up to 1,200 min, and at temperatures in the range of 2 to 37 C. When any of these anesthetics were applied for 300 min at 1.25 X saturation, a substantial decrease in number of survivors occurred. Halothane was most bactericidal, cyclopropane and Ethrane were moderately bactericidal, and t-12 was least bactericidal. At saturation values of less than 1.0, none of the four anesthetics had an appreciable effect on viability of E. coli. Greatest increases in cell permeability occurred when anesthetics were used at saturation values of 1.25, and permeability changes generally decreased as the concentrations of the chemicals were reduced. In many instances, anesthetics in the vapor state caused significant increases in cell permeability but little or no loss of viability. This indicated that a close relationship did not exist between loss of viability and increased permeability. All four anesthetics caused E. coli to lose substantial and similar amounts of compounds absorbing at 260 nm. Release of compounds absorbing at 260 nm generally increased as the saturation value of a given chemical was increased. Halothane, Ethrane, and cyclopropane but not f-12 caused lysis of E. coli ML300. Considering all results, E. coli ML30 was damaged more by halothane or cyclopropane than by f-12 or Ethrane. When f-12 was applied at a saturation value of 1.25, the bactericidal effect on E. coli was much greater at 37 or 22 C than at 12 or 2 C."}
{"id": "PMID:1099987", "title": "Antibodies against enteric bacteria in brown bullhead catfish (Ictalurus nebulosus, LeSueur) inhabiting contaminated waters.", "content": "Brown bullhead catfish were collected from sewage- and acid mine waste-polluted waters in an attempt to detect antibodies against human enteric bacteria in their sera and to investigate the association of antibody response with environmental conditions. Agglutination antigens prepared from isolates obtained from water collected at the same locations as the fish habitats were used to demonstrate such antibodies. The results showed large percentages of reactive sera for common isolates such as Escherichia coli and Enterobacter cloacae as well as lesser incidences of antibodies to other, less common isolates. In general, fish with the highest titres were collected from habitats with higher coliform counts. Acid mine drainage reduced the total coliform counts, but did not appear to affect the titers of sera from fish collected from water so affected. It was concluded that the bottom-feeding catfish might be a better subject for the study of fish as an ecological indicator of fecal pollution in acid-polluted waters.", "contents": "Antibodies against enteric bacteria in brown bullhead catfish (Ictalurus nebulosus, LeSueur) inhabiting contaminated waters. Brown bullhead catfish were collected from sewage- and acid mine waste-polluted waters in an attempt to detect antibodies against human enteric bacteria in their sera and to investigate the association of antibody response with environmental conditions. Agglutination antigens prepared from isolates obtained from water collected at the same locations as the fish habitats were used to demonstrate such antibodies. The results showed large percentages of reactive sera for common isolates such as Escherichia coli and Enterobacter cloacae as well as lesser incidences of antibodies to other, less common isolates. In general, fish with the highest titres were collected from habitats with higher coliform counts. Acid mine drainage reduced the total coliform counts, but did not appear to affect the titers of sera from fish collected from water so affected. It was concluded that the bottom-feeding catfish might be a better subject for the study of fish as an ecological indicator of fecal pollution in acid-polluted waters."}
{"id": "PMID:1099988", "title": "Principal component analysis of infraspecific variation in bacteria.", "content": "In certain types of ecological investigations it may be desirable to investigate infraspecific variation in bacteria. Principal component analysis is demonstrated to be satisfactory for this purpose. Hypothetical bacterial populations were used to show that such analysis can be used to compare collections of bacterial isolates taken at different times or from different sources. Alternatively, given n isolates, whether they represent a single bacterial population can be determined. The method is applied to authentic collections of bacteria in three separate analyses. The results are compatible with current taxonomic tenets.", "contents": "Principal component analysis of infraspecific variation in bacteria. In certain types of ecological investigations it may be desirable to investigate infraspecific variation in bacteria. Principal component analysis is demonstrated to be satisfactory for this purpose. Hypothetical bacterial populations were used to show that such analysis can be used to compare collections of bacterial isolates taken at different times or from different sources. Alternatively, given n isolates, whether they represent a single bacterial population can be determined. The method is applied to authentic collections of bacteria in three separate analyses. The results are compatible with current taxonomic tenets."}
{"id": "PMID:1099989", "title": "Total rinse method for microbiological sampling of the internal cavity of eviscerated broiler carcasses.", "content": "A method is described for spray-rinse sampling the entire visceral cavity of broiler chicken carcasses for microbiological analyses. The method is designed to provide a more comprehensive sample than swabbing or excision of small areas.", "contents": "Total rinse method for microbiological sampling of the internal cavity of eviscerated broiler carcasses. A method is described for spray-rinse sampling the entire visceral cavity of broiler chicken carcasses for microbiological analyses. The method is designed to provide a more comprehensive sample than swabbing or excision of small areas."}
{"id": "PMID:1099991", "title": "Immunologic phenomena in herpes gestationis.", "content": "Immunofluorescence findings are of diagnostic significance in herpes gestationis. The studies in two cases demonstrated some complement components (chiefly C3 and C4, but no C(1q)), without immunoglobulins at the basement membrane zone and with no circulating anti BMZ antibodies. In one case IgG were found at the BM zone in one biopsy specimen, but were not demonstrable in several other biopsies. The IF findings seem to indicate both the alternate and classical pathways of complement activation, and speak in favor of a relation between herpes gestationis and bullous pemphigoid, in which sometimes the immunoglobulins are also not demonstrable and the immunofluorescent pattern is very much like that of herpes gestationis.", "contents": "Immunologic phenomena in herpes gestationis. Immunofluorescence findings are of diagnostic significance in herpes gestationis. The studies in two cases demonstrated some complement components (chiefly C3 and C4, but no C(1q)), without immunoglobulins at the basement membrane zone and with no circulating anti BMZ antibodies. In one case IgG were found at the BM zone in one biopsy specimen, but were not demonstrable in several other biopsies. The IF findings seem to indicate both the alternate and classical pathways of complement activation, and speak in favor of a relation between herpes gestationis and bullous pemphigoid, in which sometimes the immunoglobulins are also not demonstrable and the immunofluorescent pattern is very much like that of herpes gestationis."}
{"id": "PMID:1099993", "title": "Long term studies of pancreas transplantation in experimental diabetes mellitus.", "content": "Alloxan diabetes was induced in inbred rats that then were divided into four groups consisting of unoperated diabetic controls, sham-operated diabetic controls, rats given pancreaticoduodenal isografts, and rats given duct-ligated pancreas isografts. The animals were studied for from 18 months (controls) to two years (transplants) and the following important results were obtained: 1) In striking contrast to the diabetic controls, pancreas transplants of both types produced immediate and permanent relief of hyperglycemia, immediate and lasting elevation of serum insulin levels, a normal weight and growth curve, and good health for two years. Removal of the graft was followed by recurrence of severe diabetes. 2) Pancreas transplants of both types prevented the widespread and severe renal, ophthalmic and neural lesions of diabetes that were found in the diabetic controls. 3) The duct-ligated pancreas graft and pancreaticoduodenal transplant were equally effective in controlling diabetes. Ligation of the pancreatic duct was not followed by significant morphologic or clinical evidence of pancreatitis or by loss of endocrine function. 4) Portal venous drainage of the pancreas transplant was unnecessary for good endocrine function.", "contents": "Long term studies of pancreas transplantation in experimental diabetes mellitus. Alloxan diabetes was induced in inbred rats that then were divided into four groups consisting of unoperated diabetic controls, sham-operated diabetic controls, rats given pancreaticoduodenal isografts, and rats given duct-ligated pancreas isografts. The animals were studied for from 18 months (controls) to two years (transplants) and the following important results were obtained: 1) In striking contrast to the diabetic controls, pancreas transplants of both types produced immediate and permanent relief of hyperglycemia, immediate and lasting elevation of serum insulin levels, a normal weight and growth curve, and good health for two years. Removal of the graft was followed by recurrence of severe diabetes. 2) Pancreas transplants of both types prevented the widespread and severe renal, ophthalmic and neural lesions of diabetes that were found in the diabetic controls. 3) The duct-ligated pancreas graft and pancreaticoduodenal transplant were equally effective in controlling diabetes. Ligation of the pancreatic duct was not followed by significant morphologic or clinical evidence of pancreatitis or by loss of endocrine function. 4) Portal venous drainage of the pancreas transplant was unnecessary for good endocrine function."}
{"id": "PMID:1099994", "title": "New surgical concepts resulting from cranio-orbito-facial surgery.", "content": "The authors have defined the subspecialty of craniofacial surgery and described the organization of the multi-disciplinary team required to care for such patients. Common features of the craniofacial patient have been summarized and three major categories of patients have been proposed. These are: I. Syndromes associated with hypertelorism; II. Syndromes associated with premature synostoses or growth arrests; III. Syndromes associated with primarily mid- and lower face anomalies. Growing out of an experience with 242 operations on 106 patients, the authors have listed 9 relatively new surgical \"principles.\" Each has led to a current surgical approach that is now being employed by the craniofacial team at The University of Virginia. A number of examples are given to show ways in which the lessons learned from the craniofacial patients are now being applied, with improved results, to patients with neoplasms, traumatic injuries, or other conditions.", "contents": "New surgical concepts resulting from cranio-orbito-facial surgery. The authors have defined the subspecialty of craniofacial surgery and described the organization of the multi-disciplinary team required to care for such patients. Common features of the craniofacial patient have been summarized and three major categories of patients have been proposed. These are: I. Syndromes associated with hypertelorism; II. Syndromes associated with premature synostoses or growth arrests; III. Syndromes associated with primarily mid- and lower face anomalies. Growing out of an experience with 242 operations on 106 patients, the authors have listed 9 relatively new surgical \"principles.\" Each has led to a current surgical approach that is now being employed by the craniofacial team at The University of Virginia. A number of examples are given to show ways in which the lessons learned from the craniofacial patients are now being applied, with improved results, to patients with neoplasms, traumatic injuries, or other conditions."}
{"id": "PMID:1099995", "title": "Correction of the transposition of the great arteries.", "content": "Inversion of the atrial flow in transposition of the great arteries is achieved in the following way: the remaining atrial septum is detached from behind, leaving it as a flap between both AV-valves. The incision is continued into the coronary sinus and the Vena magna cordis to the base of the left atrial appendage along the mitral ring. The resulting flap from the split coronary sinus is sutured to the left atrial wall in front of the left side pulmonary veins, thus forming a septum dorsal to the caval veins, diverting the left pulmonary venous blood to the right side. The atrial septal flap is reattached in front of the caval vein orifices. In 50 cases a small Dacron patch was used to complete the midportion of the new septum. Six patients died and so far we have encountered one superior Vena cava stenosis and one pulmonary vein stenosis. In 16 patients completion of the midportion of the new atrial septum was done with a partially excised flap from the right atrial wall still attached to the Vena cava inferior. This method resulted in 3 operative deaths. In 42 of 58 survivors the ECG has been repeatedly controlled 3 months to 6 years postoperatively. Thirty-six of the 42 patients have sinus rhythm, 4 vary between nodal and sinus-rhythm and 2 have a total AV-dissociation.", "contents": "Correction of the transposition of the great arteries. Inversion of the atrial flow in transposition of the great arteries is achieved in the following way: the remaining atrial septum is detached from behind, leaving it as a flap between both AV-valves. The incision is continued into the coronary sinus and the Vena magna cordis to the base of the left atrial appendage along the mitral ring. The resulting flap from the split coronary sinus is sutured to the left atrial wall in front of the left side pulmonary veins, thus forming a septum dorsal to the caval veins, diverting the left pulmonary venous blood to the right side. The atrial septal flap is reattached in front of the caval vein orifices. In 50 cases a small Dacron patch was used to complete the midportion of the new septum. Six patients died and so far we have encountered one superior Vena cava stenosis and one pulmonary vein stenosis. In 16 patients completion of the midportion of the new atrial septum was done with a partially excised flap from the right atrial wall still attached to the Vena cava inferior. This method resulted in 3 operative deaths. In 42 of 58 survivors the ECG has been repeatedly controlled 3 months to 6 years postoperatively. Thirty-six of the 42 patients have sinus rhythm, 4 vary between nodal and sinus-rhythm and 2 have a total AV-dissociation."}
{"id": "PMID:1099996", "title": "Complications of pulmonary resection.", "content": "The following complications of pulmonary resection are discussed with reference to their frequency of occurrence, etiology, diagnosis, and treatment: pulmonary insufficiency, arrhythmias, residual intrapleural air spaces, prolonged air leaks, postpneumonectomy empyema, bronchopleural fistula, cardiac herniation, lobar gangrene, esophagopleural fistula, pulmonary embolism, and tumor embolism.", "contents": "Complications of pulmonary resection. The following complications of pulmonary resection are discussed with reference to their frequency of occurrence, etiology, diagnosis, and treatment: pulmonary insufficiency, arrhythmias, residual intrapleural air spaces, prolonged air leaks, postpneumonectomy empyema, bronchopleural fistula, cardiac herniation, lobar gangrene, esophagopleural fistula, pulmonary embolism, and tumor embolism."}
{"id": "PMID:1099997", "title": "Pulmonary artery pressure and lung water during extracorporeal circulation in experimental pulmonary insufficiency.", "content": "Use of the membrane oxggenator has been advocated in the management of severe respiratory insufficiency. We have compared this method to conventional therapy in an experimental model in which 23 dogs were subjected to aspiration with 0.1 N HC1 and 18 were supported with a volume respirator and positive end-expiratory pressure or placed on partial bypass using a membrane oxygenator for 12 to 24 hours. Adequate oxygenation (POX greater than 100 mm Hg) was achieved with the membrane oxygenator. The increase in lung weight during conventional therapy was significantly greater than during membrane oxygenator support. Extracorporeal support during recovery from severe pulmonary injury allows pulmonary artery pressure to be controlled and reduces the expected increase in lung water.", "contents": "Pulmonary artery pressure and lung water during extracorporeal circulation in experimental pulmonary insufficiency. Use of the membrane oxggenator has been advocated in the management of severe respiratory insufficiency. We have compared this method to conventional therapy in an experimental model in which 23 dogs were subjected to aspiration with 0.1 N HC1 and 18 were supported with a volume respirator and positive end-expiratory pressure or placed on partial bypass using a membrane oxygenator for 12 to 24 hours. Adequate oxygenation (POX greater than 100 mm Hg) was achieved with the membrane oxygenator. The increase in lung weight during conventional therapy was significantly greater than during membrane oxygenator support. Extracorporeal support during recovery from severe pulmonary injury allows pulmonary artery pressure to be controlled and reduces the expected increase in lung water."}
{"id": "PMID:1099998", "title": "Cardiovascular effects of central microinjections of apomorphin in cats.", "content": "Microinjections of apomorphine (25-300 mug) were made into the lateral ventricle, dorsal nucleus of the vagus and ventromedial nucleus of the hypothalamus of cats through a stereotaxically implanted cannula-electrode. Apomorphine caused depressor and bradycardic effects without any dose-response relationship. At the above doses of apomorphine the efferent vagal discharges werr markedly increased concurrent with cardiovascular changes. Respiration was not affected except at higher doses causing depression in some experiments. Pretreatment with atropine, scopolamine or haloperidol abolished those responses. Similar results were observed with bivagotomy and midcollicular transection. Dopamine (125-100 mug), acetylcholine (10-100 mug) and norepinephrine (25-100 mug) caused similar cardiovascular changes, as in the case of apomorphine, and such effects were blocked by both specific and non-specific autonomic blackers. Thus the hypothalamus and the dorsal nucleus of the vagus appear to be inbolved in the central cardiovascular effects of apomorphine and such effects may be mediated through more than one neurotransmitter mechanisms.", "contents": "Cardiovascular effects of central microinjections of apomorphin in cats. Microinjections of apomorphine (25-300 mug) were made into the lateral ventricle, dorsal nucleus of the vagus and ventromedial nucleus of the hypothalamus of cats through a stereotaxically implanted cannula-electrode. Apomorphine caused depressor and bradycardic effects without any dose-response relationship. At the above doses of apomorphine the efferent vagal discharges werr markedly increased concurrent with cardiovascular changes. Respiration was not affected except at higher doses causing depression in some experiments. Pretreatment with atropine, scopolamine or haloperidol abolished those responses. Similar results were observed with bivagotomy and midcollicular transection. Dopamine (125-100 mug), acetylcholine (10-100 mug) and norepinephrine (25-100 mug) caused similar cardiovascular changes, as in the case of apomorphine, and such effects were blocked by both specific and non-specific autonomic blackers. Thus the hypothalamus and the dorsal nucleus of the vagus appear to be inbolved in the central cardiovascular effects of apomorphine and such effects may be mediated through more than one neurotransmitter mechanisms."}
{"id": "PMID:1099999", "title": "Influence of temperature and cocaine on responses of the isolated mouse vas deferens to adrenergic amines.", "content": "Lowering the bath temperature from 37 degrees C to 20 degrees C resulted in supersensitivity of the isolated mouse was deferens to norepinephrine. Dose-response curves to norepinephrine were shifted to the left along the log dose axis (enhanced potency) and the maximum degree of contraction that could be elicited was increased. The effect of cocaine on norepinephrine effects was qualitatively the same as hypothermia. Both treatments enhanced the potencies of other alpha receptor agonists that are substrates for the adrenergic neuronal membrane uptake mechanism. Neither treatment enhanced the potency of methoxamine, which is not taken up by adrenergic neurons, but hypothermia increased the methoxamine-induced maximum contractile height. Uptake of 14C-norepinephrine by the vas deferens was abolished by cocaine and low temperature. The data suggest that enhanced potency of adrenergic amines by hypothermia results from an inhibition of uptake by adrenergic neurons. Increased height of maximum contraction at low temperature may be partly due to decreased neuronal uptake, but other mechanisms are also likely to be involved in this effect.", "contents": "Influence of temperature and cocaine on responses of the isolated mouse vas deferens to adrenergic amines. Lowering the bath temperature from 37 degrees C to 20 degrees C resulted in supersensitivity of the isolated mouse was deferens to norepinephrine. Dose-response curves to norepinephrine were shifted to the left along the log dose axis (enhanced potency) and the maximum degree of contraction that could be elicited was increased. The effect of cocaine on norepinephrine effects was qualitatively the same as hypothermia. Both treatments enhanced the potencies of other alpha receptor agonists that are substrates for the adrenergic neuronal membrane uptake mechanism. Neither treatment enhanced the potency of methoxamine, which is not taken up by adrenergic neurons, but hypothermia increased the methoxamine-induced maximum contractile height. Uptake of 14C-norepinephrine by the vas deferens was abolished by cocaine and low temperature. The data suggest that enhanced potency of adrenergic amines by hypothermia results from an inhibition of uptake by adrenergic neurons. Increased height of maximum contraction at low temperature may be partly due to decreased neuronal uptake, but other mechanisms are also likely to be involved in this effect."}
{"id": "PMID:1100001", "title": "Lymphocyte function in rheumatic disorders.", "content": "This review discusses the immunology of rheumatic diseases, specifically rheumatoid arthritis, and systemic lupus erythematosus, and presents some known data, while suggesting what more needs to be learned. Cellular immunity occurring in rheumatoid arthritis and systemic lupus erythematosus is emphasized.", "contents": "Lymphocyte function in rheumatic disorders. This review discusses the immunology of rheumatic diseases, specifically rheumatoid arthritis, and systemic lupus erythematosus, and presents some known data, while suggesting what more needs to be learned. Cellular immunity occurring in rheumatoid arthritis and systemic lupus erythematosus is emphasized."}
{"id": "PMID:1100006", "title": "Cryptococcal meningitis: diagnostic value of cryptococcal antigen in cerebrospinal fluid.", "content": "In three previously reported cases of cryptococcal meningitis, the only laboratory evidence for this diagnosis was the presence of cryptococcal antigen in the cerebrospinal fluid (CSF). Three additional patients had chronic meningitis and repeatedly negative CSF cultures and had cryptococcal antigen demonstrated in the CSF. In our patients, the diagnosis was further supported by the complete recovery after amphotericin B therapy in two and the demonstration of Cryptococcus neoformans in the meninges at autopsy in the third. In certain patients with chronic meningitis, the detection of cryptococcal antigen in the CSF may be the only means of establishing a diagnosis during life. In such patients, if cryptococcal antigen is present in the CSF in a titer of larger than or equal to 1:8, antifungal therapy should be initiated, pending results of other diagnostic studies.", "contents": "Cryptococcal meningitis: diagnostic value of cryptococcal antigen in cerebrospinal fluid. In three previously reported cases of cryptococcal meningitis, the only laboratory evidence for this diagnosis was the presence of cryptococcal antigen in the cerebrospinal fluid (CSF). Three additional patients had chronic meningitis and repeatedly negative CSF cultures and had cryptococcal antigen demonstrated in the CSF. In our patients, the diagnosis was further supported by the complete recovery after amphotericin B therapy in two and the demonstration of Cryptococcus neoformans in the meninges at autopsy in the third. In certain patients with chronic meningitis, the detection of cryptococcal antigen in the CSF may be the only means of establishing a diagnosis during life. In such patients, if cryptococcal antigen is present in the CSF in a titer of larger than or equal to 1:8, antifungal therapy should be initiated, pending results of other diagnostic studies."}
{"id": "PMID:1100007", "title": "Fungal infection following renal transplantation.", "content": "Twenty-seven deep fungal infections developed in 22 of 171 patients following renal transplantation. These infections included cryptococcosis (ten), nocardiosis (seven), candidiasis (four), aspergillosis (two), phycomycosis (two), chromomycosis (one), and subcutaneous infection with Phialophora gougeroti (one). Twelve infections occurred in living-related and ten in cadaveric recipients. Nineteen of the 22 patients were male. Infections occurred from 0 to 61 months after transplantation. Complicating non-fungal infections were present concomitantly in 15 patients. Thirteen patients died, eight probably as a result of fungal infection. Appropriate diagnostic procedures yielded a diagnosis in 20 of 27 infections, and therapy was begun in 18 patients. Serologic, culture, and biopsy procedures useful in making rapid diagnoses are advocated in the hope of increasing survival.", "contents": "Fungal infection following renal transplantation. Twenty-seven deep fungal infections developed in 22 of 171 patients following renal transplantation. These infections included cryptococcosis (ten), nocardiosis (seven), candidiasis (four), aspergillosis (two), phycomycosis (two), chromomycosis (one), and subcutaneous infection with Phialophora gougeroti (one). Twelve infections occurred in living-related and ten in cadaveric recipients. Nineteen of the 22 patients were male. Infections occurred from 0 to 61 months after transplantation. Complicating non-fungal infections were present concomitantly in 15 patients. Thirteen patients died, eight probably as a result of fungal infection. Appropriate diagnostic procedures yielded a diagnosis in 20 of 27 infections, and therapy was begun in 18 patients. Serologic, culture, and biopsy procedures useful in making rapid diagnoses are advocated in the hope of increasing survival."}
{"id": "PMID:1100009", "title": "[Phagocytosis in patients with splenectomy].", "content": "Phagocytosis was evaluated in nine patients with splenctomy undergoing renal transplant, eight renal transplanted patients without splenectomy, three immunodepressed patients with terminal renal failure, four patients with splenectomy and six healthy volunteers. For phagocytosis evaluation, neutrophils adhered to glass were used. They were cover with 4% latex and albumin containing culture medium with or without phagocytosis stimulating tetrapeptide extracted from IgG in each case. Phagocytosis was decreased in all groups studied. Immunodepressed patients showed the highest diminishment of phagocytosis and patients with splenectomy and immunodepression the lesser. It was speculated that such a finding is due to a decrease of tetrapeptide in serum.", "contents": "[Phagocytosis in patients with splenectomy]. Phagocytosis was evaluated in nine patients with splenctomy undergoing renal transplant, eight renal transplanted patients without splenectomy, three immunodepressed patients with terminal renal failure, four patients with splenectomy and six healthy volunteers. For phagocytosis evaluation, neutrophils adhered to glass were used. They were cover with 4% latex and albumin containing culture medium with or without phagocytosis stimulating tetrapeptide extracted from IgG in each case. Phagocytosis was decreased in all groups studied. Immunodepressed patients showed the highest diminishment of phagocytosis and patients with splenectomy and immunodepression the lesser. It was speculated that such a finding is due to a decrease of tetrapeptide in serum."}
{"id": "PMID:1100010", "title": "[The influence of the antivitamine pyrithiamine on the metabolism of the thiamin-autotrophic Serratia marcescens].", "content": "A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to phosphorylate the antivitamine (presumably to pyrithiamine monophosphate). The uptake of the analog influences neither the generation time of Serratia marcescens nor the biomass of the culture, nor the total amounts of carbohydrate and protein within the cells. On the contrary, pyrithiamin stimulates the exponential death-rate k and decreases the quantity of neutral fats. The carbon source glycerol is consumed at a higher rate, which is paralleled by an increased total amount of pyruvate as well as by an increased biosynthesis of acetoin.", "contents": "[The influence of the antivitamine pyrithiamine on the metabolism of the thiamin-autotrophic Serratia marcescens]. A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to phosphorylate the antivitamine (presumably to pyrithiamine monophosphate). The uptake of the analog influences neither the generation time of Serratia marcescens nor the biomass of the culture, nor the total amounts of carbohydrate and protein within the cells. On the contrary, pyrithiamin stimulates the exponential death-rate k and decreases the quantity of neutral fats. The carbon source glycerol is consumed at a higher rate, which is paralleled by an increased total amount of pyruvate as well as by an increased biosynthesis of acetoin."}
{"id": "PMID:1100012", "title": "Exercise electrocardiograms: Code for their clinical evaluation.", "content": "This report suggests the use of a simple clinical method of codification of the most significant electrocardiographic changes as a result of the exercise stress test. It uses common abbreviations in the evaluation of the electrocardiographic findings before, during and after exercise. The five major categories to be codified include: (1) the basic electrocardiographic pattern, (2) the heart rate, (3) the pattern of ventricular conduction, (4) the pattern of rhythm and (5) the ST segment deviation. The initial sequence of five symbols indicates the findings in the preexercise electrocardiogram. This is followed by the word \"to\" with a  subscript number. The number in subscript indicates the peak heart rate achieved during the exercise. The sequence of symbols following \"to[]\" serves to indicate the electrocardiographic changes observed in the postexercise period. The data codified using this system have been found to be concise and easily comparable. The familiarity of the symbols used facilitates its learning and application.", "contents": "Exercise electrocardiograms: Code for their clinical evaluation. This report suggests the use of a simple clinical method of codification of the most significant electrocardiographic changes as a result of the exercise stress test. It uses common abbreviations in the evaluation of the electrocardiographic findings before, during and after exercise. The five major categories to be codified include: (1) the basic electrocardiographic pattern, (2) the heart rate, (3) the pattern of ventricular conduction, (4) the pattern of rhythm and (5) the ST segment deviation. The initial sequence of five symbols indicates the findings in the preexercise electrocardiogram. This is followed by the word \"to\" with a  subscript number. The number in subscript indicates the peak heart rate achieved during the exercise. The sequence of symbols following \"to[]\" serves to indicate the electrocardiographic changes observed in the postexercise period. The data codified using this system have been found to be concise and easily comparable. The familiarity of the symbols used facilitates its learning and application."}
{"id": "PMID:1100013", "title": "Glucocorticoid and antibiotic effect on experimental gram-negative bacteremic shock.", "content": "This study was designed to answer the three following questions: (1) Are glucocorticoids as protective in Gram-negative bacteremic shock as they are in endotoxic shock? (2) Is there any difference in efficacy between a bacteriostatic and a bactericidal antibiotic in bacteremic shock? (3) Does the combination of glucocorticoid with antibiotic potentiate the individual protective effects of both? Bacteremia was induced in male Sprague-Dawley rats by a single intravenous injection of viable Escherichia coli. The results showed that dexamethasone sodium phosphate alone afforded significant protection against Gram-negative bacteremic shock up to eight hours after challenge. The choice of a bactericidal vs a bacteriostatic antibiotic did not influence the survival rates in this study. The survival rate was maximal when dexamethasone was used with both ampicillin sodium and gentamicin sulfate.", "contents": "Glucocorticoid and antibiotic effect on experimental gram-negative bacteremic shock. This study was designed to answer the three following questions: (1) Are glucocorticoids as protective in Gram-negative bacteremic shock as they are in endotoxic shock? (2) Is there any difference in efficacy between a bacteriostatic and a bactericidal antibiotic in bacteremic shock? (3) Does the combination of glucocorticoid with antibiotic potentiate the individual protective effects of both? Bacteremia was induced in male Sprague-Dawley rats by a single intravenous injection of viable Escherichia coli. The results showed that dexamethasone sodium phosphate alone afforded significant protection against Gram-negative bacteremic shock up to eight hours after challenge. The choice of a bactericidal vs a bacteriostatic antibiotic did not influence the survival rates in this study. The survival rate was maximal when dexamethasone was used with both ampicillin sodium and gentamicin sulfate."}
{"id": "PMID:1100014", "title": "Low-dose heparin in postoperative patients: a prospective, coded study.", "content": "One hundred five patients over age 40 undergoing various major operations were randomly divided into control and treated groups; all were treated by subcutaneous injection containing either 5,000 international units aqueous heparin sodium or a placebo one hour prior to operation and every 12 hours thereafter for eight days. Deep vein thrombosis (DVT) was detected by daily -125I-fibrinogen injection and leg scanning, and confirmed by ascending phlebography. Both groups were comparably distributed by age, sex, variety of operation, incidence of previous thromboembolism, and myocardial and cerebrovascular disease. Blood loss was not increased in the treated group. Incidence of DVT was 8.6 percent for the total group, 7.5 percent in the heparin-tested group (four of 53), and 9.6 percent in the control group (five of 52), including one control patient with a normal scan (who later had a pulmonary embolus.", "contents": "Low-dose heparin in postoperative patients: a prospective, coded study. One hundred five patients over age 40 undergoing various major operations were randomly divided into control and treated groups; all were treated by subcutaneous injection containing either 5,000 international units aqueous heparin sodium or a placebo one hour prior to operation and every 12 hours thereafter for eight days. Deep vein thrombosis (DVT) was detected by daily -125I-fibrinogen injection and leg scanning, and confirmed by ascending phlebography. Both groups were comparably distributed by age, sex, variety of operation, incidence of previous thromboembolism, and myocardial and cerebrovascular disease. Blood loss was not increased in the treated group. Incidence of DVT was 8.6 percent for the total group, 7.5 percent in the heparin-tested group (four of 53), and 9.6 percent in the control group (five of 52), including one control patient with a normal scan (who later had a pulmonary embolus."}
{"id": "PMID:1100015", "title": "The leukocyte aggregation test: immunodiagnostic applications and immunotherapeutic implications for clinical renal transplantation.", "content": "The leukocyte aggregation test (LAT) detects the in vitro adhesion of sensitized, but not nonimmune, recipient leukocytes onto donor kidney cell monolayers. The test specifically detects cell-mediated homograft immunity up to 15 days prior to the appearance of clinical signs or alteration of chemical indexes. The presence of a positive reaction always signified incipient homograft rejection, which was usually controlled by intravenously administered, high-dose methylprednisolone sodium succinate (Solu-Medrol) therapy. There was no instance in which methyl-prednisolone treatment effectively reversed rejection in the presence of a negative leukocyte aggregation test. One common form of homograft rejection may be characterized by positive LAT results, a cellular infiltrate on the renal biopsy specimen, and sensitivity to methylprednisolone therapy.", "contents": "The leukocyte aggregation test: immunodiagnostic applications and immunotherapeutic implications for clinical renal transplantation. The leukocyte aggregation test (LAT) detects the in vitro adhesion of sensitized, but not nonimmune, recipient leukocytes onto donor kidney cell monolayers. The test specifically detects cell-mediated homograft immunity up to 15 days prior to the appearance of clinical signs or alteration of chemical indexes. The presence of a positive reaction always signified incipient homograft rejection, which was usually controlled by intravenously administered, high-dose methylprednisolone sodium succinate (Solu-Medrol) therapy. There was no instance in which methyl-prednisolone treatment effectively reversed rejection in the presence of a negative leukocyte aggregation test. One common form of homograft rejection may be characterized by positive LAT results, a cellular infiltrate on the renal biopsy specimen, and sensitivity to methylprednisolone therapy."}
{"id": "PMID:1100017", "title": "[Caudal vertebro-medullary dysrhaphia. Intra-sacral forms].", "content": "The mean theories of the sacral spinal dysraphisms are reviewed and 4 cases of \"meningeal intrasacral diverticulum\" are reported. The authors prefer this term to the more common \"intrasacral occult meningocele\". The proeminent symptom in their cases was late vesical incontinence, which disappeared after surgery. In the pertinent literature 24 similar cases were described till now.", "contents": "[Caudal vertebro-medullary dysrhaphia. Intra-sacral forms]. The mean theories of the sacral spinal dysraphisms are reviewed and 4 cases of \"meningeal intrasacral diverticulum\" are reported. The authors prefer this term to the more common \"intrasacral occult meningocele\". The proeminent symptom in their cases was late vesical incontinence, which disappeared after surgery. In the pertinent literature 24 similar cases were described till now."}
{"id": "PMID:1100019", "title": "Use of the Lombard response in cases of hysterical aphonia.", "content": "Lombard is best known for his work on the effect of noise on vocal intensity. In 1917, he applied this concept to the treatment of hysterical aphonia and described the procedure he used in a military publication. He suggested that a masking noise will interfere with the auditory monitoring system sufficiently that the patient will not be aware he has regained use of phonation. Removal of the noise from the patient's ears confirms the return of phonation, and the aphonia disappears. Credit is given to Lombard for this pioneering effort, and suggestions for present-day clinical use are made.", "contents": "Use of the Lombard response in cases of hysterical aphonia. Lombard is best known for his work on the effect of noise on vocal intensity. In 1917, he applied this concept to the treatment of hysterical aphonia and described the procedure he used in a military publication. He suggested that a masking noise will interfere with the auditory monitoring system sufficiently that the patient will not be aware he has regained use of phonation. Removal of the noise from the patient's ears confirms the return of phonation, and the aphonia disappears. Credit is given to Lombard for this pioneering effort, and suggestions for present-day clinical use are made."}
{"id": "PMID:1100020", "title": "Membranous glomerulopathy in a patient with sarcoidosis.", "content": "We describe a case of membranous nephropathy in a patient with pulmonary, splenic and hepatic sarcoidosis. The patient was asymptomatic, and edema was absent notwithstanding the proteinuria (over 8.0 gm/100 ml daily). Prednisone cleared the pulmonary and splenic complications, but the proteinuria, although diminished, persisted. Adjunctive therapy with cyclophosphamide caused further diminishment of the proteinuria. We have reviewed the relationship between the nephropathy and the sarcoidosis and suggest that a causal relationship exists between the two diseases.", "contents": "Membranous glomerulopathy in a patient with sarcoidosis. We describe a case of membranous nephropathy in a patient with pulmonary, splenic and hepatic sarcoidosis. The patient was asymptomatic, and edema was absent notwithstanding the proteinuria (over 8.0 gm/100 ml daily). Prednisone cleared the pulmonary and splenic complications, but the proteinuria, although diminished, persisted. Adjunctive therapy with cyclophosphamide caused further diminishment of the proteinuria. We have reviewed the relationship between the nephropathy and the sarcoidosis and suggest that a causal relationship exists between the two diseases."}
{"id": "PMID:1100021", "title": "Granulomatous pneumonitis. A result of intrapleural instillation of quinacrine and talcum powder.", "content": "A 73-year-old man with bilateral recurrent pleural effusions had relief of symptoms with intrapleural instillation of quinacrine hydrochloride and talcum powder. At postmortem, examination of the lungs showed granulomatous consolidation of a portion of the lower lobe of the left lung. The granulomatous reaction was in response to large numbers of talc and quinacrine crystals within the pulmonary parenchyma. A review of the literature disclosed no reports of pulmonary damage following the intrapleural administration of these two agents. This unusual complication of therapy may have resulted from the aspiration of crystals through a small, undetected bronchopleural fistula.", "contents": "Granulomatous pneumonitis. A result of intrapleural instillation of quinacrine and talcum powder. A 73-year-old man with bilateral recurrent pleural effusions had relief of symptoms with intrapleural instillation of quinacrine hydrochloride and talcum powder. At postmortem, examination of the lungs showed granulomatous consolidation of a portion of the lower lobe of the left lung. The granulomatous reaction was in response to large numbers of talc and quinacrine crystals within the pulmonary parenchyma. A review of the literature disclosed no reports of pulmonary damage following the intrapleural administration of these two agents. This unusual complication of therapy may have resulted from the aspiration of crystals through a small, undetected bronchopleural fistula."}
{"id": "PMID:1100022", "title": "Changes in the composition of a nickel-base partial denture casting alloy upon fusion and casting.", "content": "Three series of tensile test pieces were produced using a nickel-base partial denture casting alloy. For the first series induction heating was employed for melting the alloy, for the second a resistance crucible, and for the third an oxy-acetylene torch. In each series the same metal was cast sequentially five times, following which samples of the alloy were subjected to a ten element quantitative analysis to ascertain compositional changes associated with the three methods of fusion.", "contents": "Changes in the composition of a nickel-base partial denture casting alloy upon fusion and casting. Three series of tensile test pieces were produced using a nickel-base partial denture casting alloy. For the first series induction heating was employed for melting the alloy, for the second a resistance crucible, and for the third an oxy-acetylene torch. In each series the same metal was cast sequentially five times, following which samples of the alloy were subjected to a ten element quantitative analysis to ascertain compositional changes associated with the three methods of fusion."}
{"id": "PMID:1100023", "title": "The effects of remelting on the mechanical properties of a nickel base partial denture casting alloy.", "content": "Three series of tensile test pieces were produced using a nickel base partial denture casting alloy. For the first series induction heating was employed, for the second a resistance crucible, and for the third, an oxy-acetylene torch. In each series the same metal was cast sequentially a number of times and all test pieces so produced were subjected to mechanical testing. The mechanical properties were found to vary according to both the number of times the alloy was cast and the method of heating used to render the alloy molten.", "contents": "The effects of remelting on the mechanical properties of a nickel base partial denture casting alloy. Three series of tensile test pieces were produced using a nickel base partial denture casting alloy. For the first series induction heating was employed, for the second a resistance crucible, and for the third, an oxy-acetylene torch. In each series the same metal was cast sequentially a number of times and all test pieces so produced were subjected to mechanical testing. The mechanical properties were found to vary according to both the number of times the alloy was cast and the method of heating used to render the alloy molten."}
{"id": "PMID:1100024", "title": "The pulpless abutment.", "content": "The use of preformed metallic anchorages inserted in satisfactorily treated teeth for increased stabilization and retention in complete and partial dentures is demonstrated. An additonal advantage is the retention of proprioceptive guidance in mastication with complete dentures. A number of clinical examples are illustrated.", "contents": "The pulpless abutment. The use of preformed metallic anchorages inserted in satisfactorily treated teeth for increased stabilization and retention in complete and partial dentures is demonstrated. An additonal advantage is the retention of proprioceptive guidance in mastication with complete dentures. A number of clinical examples are illustrated."}
{"id": "PMID:1100025", "title": "Microporosity in casting alloys.", "content": "Three series of tensile test pieces were produced using a nickel base partial denture casting alloy. For the first series induction heating was employed, for the second a resistance crucible, and for the third an oxy-acetylene torch. Samples from each series were sectioned longitudinally, mounted, polished and examined microscopically for evidence of microporosity.", "contents": "Microporosity in casting alloys. Three series of tensile test pieces were produced using a nickel base partial denture casting alloy. For the first series induction heating was employed, for the second a resistance crucible, and for the third an oxy-acetylene torch. Samples from each series were sectioned longitudinally, mounted, polished and examined microscopically for evidence of microporosity."}
{"id": "PMID:1100026", "title": "Periodontal treatment requirements of a group of defence forces' personnel.", "content": "The periodontal treatment requirements of 187 Royal Australian Air Force men aged 18-54 years based on clinical signs and symptoms were assessed. Every man required some treatment; 60 per cent needed surgery in one or more segments of the mouth.", "contents": "Periodontal treatment requirements of a group of defence forces' personnel. The periodontal treatment requirements of 187 Royal Australian Air Force men aged 18-54 years based on clinical signs and symptoms were assessed. Every man required some treatment; 60 per cent needed surgery in one or more segments of the mouth."}
{"id": "PMID:1100027", "title": "Papillary necrosis in renal allografts--report of 2 cases.", "content": "Two patients are reported in whom early and irreversible renal allograft rejection was associated with passage of necrotic papillary material in the urine. Evidence is presented for a causal relationship between rejection and medullary necrosis. Passage of a papilla is regarded as a definite indication for graft exploration.", "contents": "Papillary necrosis in renal allografts--report of 2 cases. Two patients are reported in whom early and irreversible renal allograft rejection was associated with passage of necrotic papillary material in the urine. Evidence is presented for a causal relationship between rejection and medullary necrosis. Passage of a papilla is regarded as a definite indication for graft exploration."}
{"id": "PMID:1100028", "title": "Aortic valve replacement following cadaver renal transplantation.", "content": "Acute bacterial endocarditis producing aortic incompetence developed in a patient undergoin haemodialysis.  Subsequent cadaver renal transplantation and aortic valve replacement has proved successful.", "contents": "Aortic valve replacement following cadaver renal transplantation. Acute bacterial endocarditis producing aortic incompetence developed in a patient undergoin haemodialysis.  Subsequent cadaver renal transplantation and aortic valve replacement has proved successful."}
{"id": "PMID:1100029", "title": "Cold agglutinin formation in patients undergoing haemodialysis.  A possible relationship to dialyser re-use.", "content": "Cold agglutinins with anti-N characteristics were shown to develop in as short a time as four months in patients on home dialysis.  It appeared that the development of such antibodies is related to dialyser re-use.  Those who were treated without re-using their dialyser did not develop these antibodies.  Anti-N may cause early graft failure associated with agglutination in a cold allograft.  Moreover, if these antibodies develop in patients on dialysis there may be problems with extracorporeal dialysis and blood transfusion.", "contents": "Cold agglutinin formation in patients undergoing haemodialysis.  A possible relationship to dialyser re-use. Cold agglutinins with anti-N characteristics were shown to develop in as short a time as four months in patients on home dialysis.  It appeared that the development of such antibodies is related to dialyser re-use.  Those who were treated without re-using their dialyser did not develop these antibodies.  Anti-N may cause early graft failure associated with agglutination in a cold allograft.  Moreover, if these antibodies develop in patients on dialysis there may be problems with extracorporeal dialysis and blood transfusion."}
{"id": "PMID:1100030", "title": "The pituitary-testicular response to luteinising hormone releasing hormone administration to normal men.", "content": "The response of the pituitary and testis in normal men to the injection of LH-RH has been studied using single intravenous doses of either 25 mug or 100 mug.  Serum LH levels rose with both doses but significant elevation of plasma testosterone was evident only following 100 mug of LH-RH.  Though testosterone levels were elevated within 60 minutes, peak responses occurred from 4-12 hours post-injection.  The rise in serum FSH levels was small or absent at 25 mug but was clearly present following 100 mug LH-RH, in general rising more slowly than LH levels but persisting for a longer period.", "contents": "The pituitary-testicular response to luteinising hormone releasing hormone administration to normal men. The response of the pituitary and testis in normal men to the injection of LH-RH has been studied using single intravenous doses of either 25 mug or 100 mug.  Serum LH levels rose with both doses but significant elevation of plasma testosterone was evident only following 100 mug of LH-RH.  Though testosterone levels were elevated within 60 minutes, peak responses occurred from 4-12 hours post-injection.  The rise in serum FSH levels was small or absent at 25 mug but was clearly present following 100 mug LH-RH, in general rising more slowly than LH levels but persisting for a longer period."}
{"id": "PMID:1100038", "title": "Hemodynamics, renal function, plasma renin, and aldosterone in man after 5 to 14 days of bedrest.", "content": "Continous bedrest for 5 to 14 days had no significant effect on resting heart rate, blood pressure or cardiac output in six normal men. Head-up tilt induced greater tachycardia in 5 of 6 patients after bed rest than in the control period. Propranolol diminished both the tachycardia and the incidence of hypotension and faintness in upright posture. Body weight, serum electrolytes and resting renal plasma flow, and glomerular filtration rate were unchanged by bedrest. Plasma volume fell, extracellular fluid volume increased, and plasma renin activity was significantly elevated following bedrest. Unusually large increases in plasma renin followed head-up tilt or administration of isoproterenol during bedrest, and after resuming normal activity. During bedrest, plasma aldosterone was often increased in the early morning. We conclude that after bedrest, upright posture evokes strong beta-adrenergic activity, with exaggerated metabolic and circulatory responses which can be reduced or abolished by the beta-adrenergic blocker, propranolol.", "contents": "Hemodynamics, renal function, plasma renin, and aldosterone in man after 5 to 14 days of bedrest. Continous bedrest for 5 to 14 days had no significant effect on resting heart rate, blood pressure or cardiac output in six normal men. Head-up tilt induced greater tachycardia in 5 of 6 patients after bed rest than in the control period. Propranolol diminished both the tachycardia and the incidence of hypotension and faintness in upright posture. Body weight, serum electrolytes and resting renal plasma flow, and glomerular filtration rate were unchanged by bedrest. Plasma volume fell, extracellular fluid volume increased, and plasma renin activity was significantly elevated following bedrest. Unusually large increases in plasma renin followed head-up tilt or administration of isoproterenol during bedrest, and after resuming normal activity. During bedrest, plasma aldosterone was often increased in the early morning. We conclude that after bedrest, upright posture evokes strong beta-adrenergic activity, with exaggerated metabolic and circulatory responses which can be reduced or abolished by the beta-adrenergic blocker, propranolol."}
{"id": "PMID:1100039", "title": "Human assay of antimotion sickness drugs.", "content": "The present study was undertaken to improve previous testing procedures, involving the use of a slow rotation room, for assessing the efficacy of antimotion sickness drugs which had validity for groups of subjects but not for each individual in the group. Three major changes were introduced: first, the use of an incremental increase in the intensity of the stressful stimuli of constant intensity; second, a systematic distribution of placebos, rather than a random distribution, in using a modified Latin-square design; third, categorizing the responses as \"inconsquential,\" \"beneficial,\" and \"detrimental\"--the range of the \"placebo responses\" was doubled to define the inconsequential range and response above or below were, respectively, beneficial or detrimental. Only drugs known to have antimotion sickness effectiveness were tested and the cardinal findings can be briefly summarized: 1) The group responses were similar to the data previously reported; 2) Great individual differences in response to antimotion sickness drugs were revealed, implying that individual assessments must be made for maximal benefits; 3) The fixed-dose combination of promethazine hydrochloride and ephedrine sulfate (25 mg each) proved to be outstanding as this combination of homergic drugs clearly exhibited a suprasummation effect; and 4) A few tests were conducted using larger than usual doses and the results supported previous findings that, for a maximal beneficial effect in response to a single dose, individuals may vary both with regard to the choice of drug and the amount administered.", "contents": "Human assay of antimotion sickness drugs. The present study was undertaken to improve previous testing procedures, involving the use of a slow rotation room, for assessing the efficacy of antimotion sickness drugs which had validity for groups of subjects but not for each individual in the group. Three major changes were introduced: first, the use of an incremental increase in the intensity of the stressful stimuli of constant intensity; second, a systematic distribution of placebos, rather than a random distribution, in using a modified Latin-square design; third, categorizing the responses as \"inconsquential,\" \"beneficial,\" and \"detrimental\"--the range of the \"placebo responses\" was doubled to define the inconsequential range and response above or below were, respectively, beneficial or detrimental. Only drugs known to have antimotion sickness effectiveness were tested and the cardinal findings can be briefly summarized: 1) The group responses were similar to the data previously reported; 2) Great individual differences in response to antimotion sickness drugs were revealed, implying that individual assessments must be made for maximal benefits; 3) The fixed-dose combination of promethazine hydrochloride and ephedrine sulfate (25 mg each) proved to be outstanding as this combination of homergic drugs clearly exhibited a suprasummation effect; and 4) A few tests were conducted using larger than usual doses and the results supported previous findings that, for a maximal beneficial effect in response to a single dose, individuals may vary both with regard to the choice of drug and the amount administered."}
{"id": "PMID:1100040", "title": "Surveillance of some infectious diseases among aircrew personnel in Southeast Asia.", "content": "A 2-year analysis of specimens among aircrew personnel in Southeast Asia is reported. Stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (SGOT) and antibodies to Entamoeba histolytica, Toxoplasma gondii, Chikungunya and Japanese encephalitis viruses, Rickettsia tsutsugamushi, Leptospira sp. and Pseudomonas pseudomallei. One to four specimens each were obtained from 537 adult males and 20 females. There were 56 subjects with intestinal parasites as follows: Giardia lamblia (3.8%), Endolimax nana (2.6%), Entamoeba histolytica (1.4%), Entamoeba hartmanni (0.9%), Entamoeba coli (0.7%), Trichiuris trichiura (1.7%), Ascaris lumbricoides (1.4%), hookworm (0.9%), and Clonorchis sinensis (1.2%). Two individuals had malaria, Plasmodium vivax (0.6%). Pathogenic enteric bacteria were isolated from seven stool specimens; Shigella groups B and D (0.9%), Salmonella paratyphi (0.3%), and Arizona group (0.9%). Significantly elevated SGOT levels were found in 0.9% and antibodies to Japanese encephalitis virus in 1.5%, to Rickettsia tsutsugamushi in 1.2%, to Pseudomonas pseudomallei in 0.3%, to Entamoeba histolytica in 0.9% and to Toxoplasma gondii in 10.1%. No antibodies were found to Chikungunya virus or Leptospira sp.", "contents": "Surveillance of some infectious diseases among aircrew personnel in Southeast Asia. A 2-year analysis of specimens among aircrew personnel in Southeast Asia is reported. Stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (SGOT) and antibodies to Entamoeba histolytica, Toxoplasma gondii, Chikungunya and Japanese encephalitis viruses, Rickettsia tsutsugamushi, Leptospira sp. and Pseudomonas pseudomallei. One to four specimens each were obtained from 537 adult males and 20 females. There were 56 subjects with intestinal parasites as follows: Giardia lamblia (3.8%), Endolimax nana (2.6%), Entamoeba histolytica (1.4%), Entamoeba hartmanni (0.9%), Entamoeba coli (0.7%), Trichiuris trichiura (1.7%), Ascaris lumbricoides (1.4%), hookworm (0.9%), and Clonorchis sinensis (1.2%). Two individuals had malaria, Plasmodium vivax (0.6%). Pathogenic enteric bacteria were isolated from seven stool specimens; Shigella groups B and D (0.9%), Salmonella paratyphi (0.3%), and Arizona group (0.9%). Significantly elevated SGOT levels were found in 0.9% and antibodies to Japanese encephalitis virus in 1.5%, to Rickettsia tsutsugamushi in 1.2%, to Pseudomonas pseudomallei in 0.3%, to Entamoeba histolytica in 0.9% and to Toxoplasma gondii in 10.1%. No antibodies were found to Chikungunya virus or Leptospira sp."}
{"id": "PMID:1100041", "title": "Effects of pyrobenzamine and plimasin on fighter pilots flying a fighter intercept mission in the F4D flight simulator.", "content": "There were 26 F4D crews from a Tactical Air Force Wing which participated in a difficult intercept mission in a F4D flight simulator. They were divided into three groups, medicated with either Plimasin, Pyrobenzamine or placebo. The groups medicated with either Plimasin or Pyrobenzamine alone demonstrated decreased effectiveness in completing this intercept as compared with the nonmedicated group. These medications apparently caused impairment of mission performance and should be avoided while performing flying duties.", "contents": "Effects of pyrobenzamine and plimasin on fighter pilots flying a fighter intercept mission in the F4D flight simulator. There were 26 F4D crews from a Tactical Air Force Wing which participated in a difficult intercept mission in a F4D flight simulator. They were divided into three groups, medicated with either Plimasin, Pyrobenzamine or placebo. The groups medicated with either Plimasin or Pyrobenzamine alone demonstrated decreased effectiveness in completing this intercept as compared with the nonmedicated group. These medications apparently caused impairment of mission performance and should be avoided while performing flying duties."}
{"id": "PMID:1100037", "title": "Induced systemic candidiasis in young broiler chickens.", "content": "Systemic candidiasis was induced in broiler chickens 14 days old by intravenous injection of a suspension of viable Candida albicans cells. Injection resulted in decreased body weight, moderate mortality, swollen and reddened livers and kidneys, pancreatitis, and disturbances of the nervous system. Three types of neutral disturbances were observed: 1) extreme opisthotonus with spasmodic tremors; 2) extreme torticollis with cranial rotation of 270 degrees: and 3) extreme torticolis which resulted in the head being drawn in a medial-ventral direction. None to 50% of the inoculated birds exhibited these neural disturbances, depending on the isolant of C. albicans used. Microbiological examination of internal organs and blood revealed that C. albicans localized in the meninges of the brain. There was also a significant isolant-related effect of C. albicans on the growth rate of the inoculated birds. These easily quantitated differential effects of various isolants of C. albicans offer the prospect of correlating biochemical characteristics with virulence and obtaining information about the mechanism of pathogenicity of this microorganism.", "contents": "Induced systemic candidiasis in young broiler chickens. Systemic candidiasis was induced in broiler chickens 14 days old by intravenous injection of a suspension of viable Candida albicans cells. Injection resulted in decreased body weight, moderate mortality, swollen and reddened livers and kidneys, pancreatitis, and disturbances of the nervous system. Three types of neutral disturbances were observed: 1) extreme opisthotonus with spasmodic tremors; 2) extreme torticollis with cranial rotation of 270 degrees: and 3) extreme torticolis which resulted in the head being drawn in a medial-ventral direction. None to 50% of the inoculated birds exhibited these neural disturbances, depending on the isolant of C. albicans used. Microbiological examination of internal organs and blood revealed that C. albicans localized in the meninges of the brain. There was also a significant isolant-related effect of C. albicans on the growth rate of the inoculated birds. These easily quantitated differential effects of various isolants of C. albicans offer the prospect of correlating biochemical characteristics with virulence and obtaining information about the mechanism of pathogenicity of this microorganism."}
{"id": "PMID:1100046", "title": "Regulation of normal hemopoiesis in the acute leukemia L5222.", "content": "Normal hemopoiesis becomes markedly depressed in rats during the development of the rat leukemia L5222. Whether this could be due to the influence of a circulating humoral factor was investigated by comparing the growth of normal bone marrow cells in diffusion chambers implanted into the peritoneal cavity of normal or leukemic hosts. Similar growth in total cell numbers was observed in both groups of hosts, and no significant difference could be detected between them. In an attempt to exclude the possibility that an inhibitor either did not penetrate to the peritoneum or was inactivated by the chamber membrane, normal serum, or serum from a highly leukemic rat was mixed with the bone marrow cell suspension in the chambers. Again, no difference in growth between the 2 groups could be detected. Therefore, the influence of a circulating humoral factor in the depression of normal bone marrow hemopoiesis in this experimental leukemia seems to be ruled out, and the decrease may be attributable to local events in the bone marrow such as short-range factors or cellular interaction between the leukemic and normal hemopoietic cell populations.", "contents": "Regulation of normal hemopoiesis in the acute leukemia L5222. Normal hemopoiesis becomes markedly depressed in rats during the development of the rat leukemia L5222. Whether this could be due to the influence of a circulating humoral factor was investigated by comparing the growth of normal bone marrow cells in diffusion chambers implanted into the peritoneal cavity of normal or leukemic hosts. Similar growth in total cell numbers was observed in both groups of hosts, and no significant difference could be detected between them. In an attempt to exclude the possibility that an inhibitor either did not penetrate to the peritoneum or was inactivated by the chamber membrane, normal serum, or serum from a highly leukemic rat was mixed with the bone marrow cell suspension in the chambers. Again, no difference in growth between the 2 groups could be detected. Therefore, the influence of a circulating humoral factor in the depression of normal bone marrow hemopoiesis in this experimental leukemia seems to be ruled out, and the decrease may be attributable to local events in the bone marrow such as short-range factors or cellular interaction between the leukemic and normal hemopoietic cell populations."}
{"id": "PMID:1100047", "title": "Murine and human leukemias.", "content": "Essentially all the drugs which are active against human leukemias and lymphomas are active against one type or another of the rodent leukemias and lymphomas. Leukemia L1210 has been generally the most successful screening tool for clinically active compounds. Leukemia P388, however, seems to be better in detecting active antibiotics and natural products and P1534 is particularly sensitive to the Vinca alkaloids, while L5178Y, EARAD, and 6C3HED are useful in detecting the activities of various asparaginase containing fractions. Cell cultures of these leukemias can demonstrate mechanism of drug action and quantitate resistance. Spontaneous AKR leukemia is a model of the advanced human disease. In these leukemias vincristine and prednisone produce a 4 log cell kill. Cytoxan and arabinosyl cytosine (Ara-C) are also effective. On the other hand drugs such as mercaptopurine (6MP) and methotrexate which are highly active in the maintenance phase of acute lymphocytic leukemia (ALL) and in L1210 have little or no activity against the AKR spontaneous system. Mouse leukemias can also detect schedule dependence, synergistic combinations, cross resistance, oral activity, and the ability of drugs to pass the blood brain barrier. A case in point is the Ara-C analog 2,2'-anhydro-arabinofuranosyl-5-fluorocytosine (AAFC) which is not schedule dependent, is active orally, is potentiated by thioguanine, and is effective against intracerebrally inoculated mouse leukemia. AAFC and its analogs might thus be a considerable improvement over Ara-C which is at the present time the most important component of the combination treatment of acute myelogenous leukemia (AML).", "contents": "Murine and human leukemias. Essentially all the drugs which are active against human leukemias and lymphomas are active against one type or another of the rodent leukemias and lymphomas. Leukemia L1210 has been generally the most successful screening tool for clinically active compounds. Leukemia P388, however, seems to be better in detecting active antibiotics and natural products and P1534 is particularly sensitive to the Vinca alkaloids, while L5178Y, EARAD, and 6C3HED are useful in detecting the activities of various asparaginase containing fractions. Cell cultures of these leukemias can demonstrate mechanism of drug action and quantitate resistance. Spontaneous AKR leukemia is a model of the advanced human disease. In these leukemias vincristine and prednisone produce a 4 log cell kill. Cytoxan and arabinosyl cytosine (Ara-C) are also effective. On the other hand drugs such as mercaptopurine (6MP) and methotrexate which are highly active in the maintenance phase of acute lymphocytic leukemia (ALL) and in L1210 have little or no activity against the AKR spontaneous system. Mouse leukemias can also detect schedule dependence, synergistic combinations, cross resistance, oral activity, and the ability of drugs to pass the blood brain barrier. A case in point is the Ara-C analog 2,2'-anhydro-arabinofuranosyl-5-fluorocytosine (AAFC) which is not schedule dependent, is active orally, is potentiated by thioguanine, and is effective against intracerebrally inoculated mouse leukemia. AAFC and its analogs might thus be a considerable improvement over Ara-C which is at the present time the most important component of the combination treatment of acute myelogenous leukemia (AML)."}
{"id": "PMID:1100053", "title": "[Influence of indole-3-alkanecarboxylic acids on glucose utilization in rats].", "content": "The influence of various indole-3-alkane-carboxylic acids (indole-3-propionic acid, indole-3-butyric acid and derivatives of this substance) on some parameters of carbohydrate, fat and insulin metabolism were studied in normal and experimentally induced diabetic rats. Earlier it had been shown, by Schillinger and Loge (1973) using rat liver slices for in vitro studies and adrenalectomized rates for in vivo experiments that indole-3-butyric acid and its analogues investigated suppress gluconeogenesis from pyruvate and that these substances have a hypoglycaemic effect when administered orally at doses of 50-250 mg/kg. Indole-3-propionic acid, indole-3-butyric acid, 1-methyl-indole-3-butyric acid, 2-methyl-indole-3-butyric acid and 1-carboxy-6-fluor-1,2,3,4-tetrahydrocarbazol, administered orally at doses up to 1000 mg/kg, had either only a slight or no hypoglycaemic effect at all in intact and streptozotocin-diabetic rats. The suppression of gluconeogenesis observed in vitro thus only has an appreciable effect on blood glucose in vivo when the capacity of the liver to synthetize glucose is reduced. In normal rats indole-3-propionic acid and indole-3-butyric acid led to a deterioration of glucose tolerance following i.v. glucose loading and to a reduction of 14C-U-glucose oxidation. An increase in the concentration of serum free fatty acids (FFA) measured after treatment with indole-3-butyric acid and its 1- and 2-methylated derivatives was not--in accordance with a theory of an interference of the glucose and fatty acid utilization published in literature--considered to be the primary cause since the reduced formation of 14CO2 from radioactive glucose is not normalized when the increased FFA content is reduced by an inhibitor of lipolysis (5-methyl-isoxazol-3-carboxylic acid)...", "contents": "[Influence of indole-3-alkanecarboxylic acids on glucose utilization in rats]. The influence of various indole-3-alkane-carboxylic acids (indole-3-propionic acid, indole-3-butyric acid and derivatives of this substance) on some parameters of carbohydrate, fat and insulin metabolism were studied in normal and experimentally induced diabetic rats. Earlier it had been shown, by Schillinger and Loge (1973) using rat liver slices for in vitro studies and adrenalectomized rates for in vivo experiments that indole-3-butyric acid and its analogues investigated suppress gluconeogenesis from pyruvate and that these substances have a hypoglycaemic effect when administered orally at doses of 50-250 mg/kg. Indole-3-propionic acid, indole-3-butyric acid, 1-methyl-indole-3-butyric acid, 2-methyl-indole-3-butyric acid and 1-carboxy-6-fluor-1,2,3,4-tetrahydrocarbazol, administered orally at doses up to 1000 mg/kg, had either only a slight or no hypoglycaemic effect at all in intact and streptozotocin-diabetic rats. The suppression of gluconeogenesis observed in vitro thus only has an appreciable effect on blood glucose in vivo when the capacity of the liver to synthetize glucose is reduced. In normal rats indole-3-propionic acid and indole-3-butyric acid led to a deterioration of glucose tolerance following i.v. glucose loading and to a reduction of 14C-U-glucose oxidation. An increase in the concentration of serum free fatty acids (FFA) measured after treatment with indole-3-butyric acid and its 1- and 2-methylated derivatives was not--in accordance with a theory of an interference of the glucose and fatty acid utilization published in literature--considered to be the primary cause since the reduced formation of 14CO2 from radioactive glucose is not normalized when the increased FFA content is reduced by an inhibitor of lipolysis (5-methyl-isoxazol-3-carboxylic acid)..."}
{"id": "PMID:1100054", "title": "[Spectral-analytical studies of the action of etifoxin on the human EEG].", "content": "Spectral analysis of EEG in healthy volunteers shows that Etifoxin, the hydrochloride of 6-chloro-4-methyl-4-phenyl-2-ethylamino-4H-3,1-benzoxazine (Hoe 36,801), in an oral dosage of 100 mg has a differential effect in function of the individual alpha organization. This differential effect appearing in the course of the quantitative evaluation can be interpreted, from a morphological point of view, as an expression of various partial phases and patterns of a process leading to reduced vigilance.", "contents": "[Spectral-analytical studies of the action of etifoxin on the human EEG]. Spectral analysis of EEG in healthy volunteers shows that Etifoxin, the hydrochloride of 6-chloro-4-methyl-4-phenyl-2-ethylamino-4H-3,1-benzoxazine (Hoe 36,801), in an oral dosage of 100 mg has a differential effect in function of the individual alpha organization. This differential effect appearing in the course of the quantitative evaluation can be interpreted, from a morphological point of view, as an expression of various partial phases and patterns of a process leading to reduced vigilance."}
{"id": "PMID:1100055", "title": "The favourable effect of adding a catalase to the treatment of syndromes due to intervertebral disc degenerations.", "content": "Over a period of one year, 648 patients suffering from 4 different but very resistant syndromes due to intervertebral disc degenerations of the cervical and lumbosacral spine were treated in an identical way, but 50% of them received also catalase (Optidase\u00bf) injections. Half of each group were males and half of them females, the age being 30--50 years. Catalase accelerates the recovery already within the first 3 weeks and the final results after 24 weeks are still significant. In addition, the study of the ischial syndrome was extended to a group of 1208 patients over a period of 5 years, confirming again the favourable effect of catalase. Catalase is generally well tolerated; dangerous reactions have not been noticed.", "contents": "The favourable effect of adding a catalase to the treatment of syndromes due to intervertebral disc degenerations. Over a period of one year, 648 patients suffering from 4 different but very resistant syndromes due to intervertebral disc degenerations of the cervical and lumbosacral spine were treated in an identical way, but 50% of them received also catalase (Optidase\u00bf) injections. Half of each group were males and half of them females, the age being 30--50 years. Catalase accelerates the recovery already within the first 3 weeks and the final results after 24 weeks are still significant. In addition, the study of the ischial syndrome was extended to a group of 1208 patients over a period of 5 years, confirming again the favourable effect of catalase. Catalase is generally well tolerated; dangerous reactions have not been noticed."}
{"id": "PMID:1100056", "title": "[Gynecological, hematological and metabolic studies during therapy with an estrogen-gestagen combination preparation].", "content": "Twenty women after menopause were submitted to substitution treatment with a biphasic hormone combination (Cyclacur\u00bf). Nine of these had undergone hysterectomy + ovarectomy. Through 11 days each of the women was administered a daily dose of 2 mg estradiol-valerianate as estrogen. Subsequently, for another 10 days each was given the identical dose of estrogen plus 0.5 mg DL-norgestrel as gestagen. Upon this followed an intermission of 7 days without any medication. After 1 cycle of placebo, 7 cycles of medication were followed by 1 cycle of placebo again with regular controls. Climacteric signs and symptoms were eliminated entirely. In those women without hysterectomy withdrawal-menorrhagia occurred promptly 2--3 days after discontinuing medication. During the entire period of investigation there was no significant change in serum lipids, blood sugar values, or serum enzyme tests used as parameter of hepatic function. As far as hematology is concerned there was a moderate rise of hematocrit. No change of serum protein-bound iodine was observed.", "contents": "[Gynecological, hematological and metabolic studies during therapy with an estrogen-gestagen combination preparation]. Twenty women after menopause were submitted to substitution treatment with a biphasic hormone combination (Cyclacur\u00bf). Nine of these had undergone hysterectomy + ovarectomy. Through 11 days each of the women was administered a daily dose of 2 mg estradiol-valerianate as estrogen. Subsequently, for another 10 days each was given the identical dose of estrogen plus 0.5 mg DL-norgestrel as gestagen. Upon this followed an intermission of 7 days without any medication. After 1 cycle of placebo, 7 cycles of medication were followed by 1 cycle of placebo again with regular controls. Climacteric signs and symptoms were eliminated entirely. In those women without hysterectomy withdrawal-menorrhagia occurred promptly 2--3 days after discontinuing medication. During the entire period of investigation there was no significant change in serum lipids, blood sugar values, or serum enzyme tests used as parameter of hepatic function. As far as hematology is concerned there was a moderate rise of hematocrit. No change of serum protein-bound iodine was observed."}
{"id": "PMID:1100057", "title": "Treatment of climacteric complaints with oestriol.", "content": "150 women with serious climacteric complaints were continuously treated from January, 1970, up to April, 1972, with Ovestin\u00bf, an orally effective preparation containing 1 mg of oestriol per tablet. After one month of treatment subjective complaints were already considerably improved. Oestrogenic activity was markedly increased, according to vaginal smears and total gonadotrophin excretion in the urine. Its high tolerance and beneficial therapeutic effect make Ovestin the preparation of choice in the treatment of the climacteric syndrome.", "contents": "Treatment of climacteric complaints with oestriol. 150 women with serious climacteric complaints were continuously treated from January, 1970, up to April, 1972, with Ovestin\u00bf, an orally effective preparation containing 1 mg of oestriol per tablet. After one month of treatment subjective complaints were already considerably improved. Oestrogenic activity was markedly increased, according to vaginal smears and total gonadotrophin excretion in the urine. Its high tolerance and beneficial therapeutic effect make Ovestin the preparation of choice in the treatment of the climacteric syndrome."}
{"id": "PMID:1100088", "title": "A preliminary report on the effects of methanol extraction residue of BCG (MER) on cancer patients.", "content": "Twenty-seven patients with malignant neoplasia were injected intradermally with the methanol extraction residue (MER) fraction of tubercle bacilli. Two schedules of treatment were used: every other week and once a month; 1-10 courses of MER were administered to the patients. The skin reactivity to 3 recall antigens, as well as to the injected MER itself, was used to monitor the immune response. Improvement of skin reactivity occurred in 9 of 18 patients tested with recall antigens. Five of 6 patients treated every other week improved in their immune capacity whereas only 4 of 12 patients improved on the monthly schedule. Thus, repeated injections given every other week were more effective in increasing the cutaneous reactivity than monthly injections of MER. The side-effects of MER treatment were tolerable.", "contents": "A preliminary report on the effects of methanol extraction residue of BCG (MER) on cancer patients. Twenty-seven patients with malignant neoplasia were injected intradermally with the methanol extraction residue (MER) fraction of tubercle bacilli. Two schedules of treatment were used: every other week and once a month; 1-10 courses of MER were administered to the patients. The skin reactivity to 3 recall antigens, as well as to the injected MER itself, was used to monitor the immune response. Improvement of skin reactivity occurred in 9 of 18 patients tested with recall antigens. Five of 6 patients treated every other week improved in their immune capacity whereas only 4 of 12 patients improved on the monthly schedule. Thus, repeated injections given every other week were more effective in increasing the cutaneous reactivity than monthly injections of MER. The side-effects of MER treatment were tolerable."}
{"id": "PMID:1100089", "title": "Historical evidence for the genetic heterogeneity of pseudoxanthoma elasticum.", "content": "Re-examination of published data from affected families with pseudoxanthoma elasticum (PXE) described in the literature between 1896 and 1968 supported the concept of clinical and genetic heterogeneity. There was clinical evidence for two autosomal dominant and one recessive type, although only a single patient with recessive type II was described. Examination of the individual pedigrees supported both autosomal dominant and recessive patterns of inheritance. The pooled genetic data were less helpful because they were so highly selected. (They had been reported in the literature.) Even so the dominant type II data fitted the hypothesis, whilst the type I and recessive groups were near the expected values. The clinical properties of the groups corresponded closely to the recent British (Pope, 1973) survey. In general, the dominant type I group showed classical cutaneous changes, vascular complications were common and the chief ophthalmic complication was a severe choroidoretinitis. The dominant type II group, in contrast, has an atypical macular rash, or sometimes no rash at all, no vascular complications and very mild retinal changes. Choroidoretinitis was rare and peau d'orange patterns and prominent choroidal vessels common. The recessive group was of intermediate severity.", "contents": "Historical evidence for the genetic heterogeneity of pseudoxanthoma elasticum. Re-examination of published data from affected families with pseudoxanthoma elasticum (PXE) described in the literature between 1896 and 1968 supported the concept of clinical and genetic heterogeneity. There was clinical evidence for two autosomal dominant and one recessive type, although only a single patient with recessive type II was described. Examination of the individual pedigrees supported both autosomal dominant and recessive patterns of inheritance. The pooled genetic data were less helpful because they were so highly selected. (They had been reported in the literature.) Even so the dominant type II data fitted the hypothesis, whilst the type I and recessive groups were near the expected values. The clinical properties of the groups corresponded closely to the recent British (Pope, 1973) survey. In general, the dominant type I group showed classical cutaneous changes, vascular complications were common and the chief ophthalmic complication was a severe choroidoretinitis. The dominant type II group, in contrast, has an atypical macular rash, or sometimes no rash at all, no vascular complications and very mild retinal changes. Choroidoretinitis was rare and peau d'orange patterns and prominent choroidal vessels common. The recessive group was of intermediate severity."}
{"id": "PMID:1100090", "title": "Erythropoietic protoprophyria--submicroscopic events during the acute photosensitivity flare.", "content": "The immediate response of erythropoietic protoporphyria (EPP) skin to long-wave ultraviolet radiation (UVR) was studied with the electron microscope. The main finding was severe vascular injury. This was confined to the superficial vessels of the dermis and consisted of endothelial cell degeneration and a pronounced leakage of vascular contents. In contrast, the epidermis showed no abnormalities. Short-wave UV irradiation of EPP skin resulted in epidermal changes typical for the usual sunburn reaction and spared the dermal blood vessels. The following conclusions are drawn: (i) Endothelial cells are the primary cellular target for the photodynamic reaction in EPP. (ii) The fibrillar material, characteristic for chronic EPP lesions, originates from the vessels and vascular contents. (iii) The multilayered basement membranes observed in such lesions reflect multiple consecutive reparative processes that follow endothelial injuries.", "contents": "Erythropoietic protoprophyria--submicroscopic events during the acute photosensitivity flare. The immediate response of erythropoietic protoporphyria (EPP) skin to long-wave ultraviolet radiation (UVR) was studied with the electron microscope. The main finding was severe vascular injury. This was confined to the superficial vessels of the dermis and consisted of endothelial cell degeneration and a pronounced leakage of vascular contents. In contrast, the epidermis showed no abnormalities. Short-wave UV irradiation of EPP skin resulted in epidermal changes typical for the usual sunburn reaction and spared the dermal blood vessels. The following conclusions are drawn: (i) Endothelial cells are the primary cellular target for the photodynamic reaction in EPP. (ii) The fibrillar material, characteristic for chronic EPP lesions, originates from the vessels and vascular contents. (iii) The multilayered basement membranes observed in such lesions reflect multiple consecutive reparative processes that follow endothelial injuries."}
{"id": "PMID:1100092", "title": "Reactions to aspirin and food additives in patients with chronic urticaria, including the physical urticarias.", "content": "In 131 patients with chronic urticaria, including physical urticarias, oral provocation tests were done with aspirin. A total of thirty-one patients showed a reaction on aspirin challenge. Reactions were seen in 35% of patients with idiopathic urticaria, 52% of patients with cholinergic urticaria, and 43% of those with pressure urticaria. The patients with reactions to aspirin were also tested with tartrazine, sodium benzoate, 4-hydroxybenzoic acid, sodium- and phenyl salicylate and the analgesics indomethacin, paracetamol and mefanamic acid. In nineteen of twenty three aspirin sensitive patients, positive reactions to one or more of these substances were observed. Indomethacin and tartrazine had the highest scores. There was no statistically significant correlation between aspirin reactions and the presence of nasal polyposis, sinusitis, asthma or atopy.", "contents": "Reactions to aspirin and food additives in patients with chronic urticaria, including the physical urticarias. In 131 patients with chronic urticaria, including physical urticarias, oral provocation tests were done with aspirin. A total of thirty-one patients showed a reaction on aspirin challenge. Reactions were seen in 35% of patients with idiopathic urticaria, 52% of patients with cholinergic urticaria, and 43% of those with pressure urticaria. The patients with reactions to aspirin were also tested with tartrazine, sodium benzoate, 4-hydroxybenzoic acid, sodium- and phenyl salicylate and the analgesics indomethacin, paracetamol and mefanamic acid. In nineteen of twenty three aspirin sensitive patients, positive reactions to one or more of these substances were observed. Indomethacin and tartrazine had the highest scores. There was no statistically significant correlation between aspirin reactions and the presence of nasal polyposis, sinusitis, asthma or atopy."}
{"id": "PMID:1100093", "title": "One hundred twin pairs patch tested with primary irritants.", "content": "The cutaneous sensitivity to benzalkonium chloride, sodium lauryl sulphate and potash soap was determined in 54 monozygotic and 46 dizygotic twin pairs. Comparing the intra-pair reaction strength a higher degree of concordance was found among monozygotic than among dizygotic twins, and even more so when monozygotic twins were compared with matched controls.", "contents": "One hundred twin pairs patch tested with primary irritants. The cutaneous sensitivity to benzalkonium chloride, sodium lauryl sulphate and potash soap was determined in 54 monozygotic and 46 dizygotic twin pairs. Comparing the intra-pair reaction strength a higher degree of concordance was found among monozygotic than among dizygotic twins, and even more so when monozygotic twins were compared with matched controls."}
{"id": "PMID:1100094", "title": "Puritus vulvae: treatment by multiple intradermal alcohol injections.", "content": "A Preliminary report is presented on the use of multiple intradermal injections of absolute alcohol in the treatment of intractable pruritus vulvae. Twenty-five patients were followed up for over I year, and of these six (24%) were cured, and thirteen (52%) showed marked symptomatic improvement.", "contents": "Puritus vulvae: treatment by multiple intradermal alcohol injections. A Preliminary report is presented on the use of multiple intradermal injections of absolute alcohol in the treatment of intractable pruritus vulvae. Twenty-five patients were followed up for over I year, and of these six (24%) were cured, and thirteen (52%) showed marked symptomatic improvement."}
{"id": "PMID:1100095", "title": "Simplified dithranol treatment for psoriasis.", "content": "Progressive simplification of dithranol therapy has made treatment at home more acceptable. This has included ommitting ultra-violet light, talc and dressings, and substituting an ordinary bath instead of one containing tar. A stiffened dithranol 0.5% ointment was found to be slightly more effective than the best paste hitherto employed. It was also very much easier to use.", "contents": "Simplified dithranol treatment for psoriasis. Progressive simplification of dithranol therapy has made treatment at home more acceptable. This has included ommitting ultra-violet light, talc and dressings, and substituting an ordinary bath instead of one containing tar. A stiffened dithranol 0.5% ointment was found to be slightly more effective than the best paste hitherto employed. It was also very much easier to use."}
{"id": "PMID:1100096", "title": "The absorption of hexachlorophane from Ultralanum ointment.", "content": "The application of Ultralanum ointment to adult skin cases has produced levels of hexachlorophane in the plasma comparable with those found in infants who have undergone whole-body washing with hexachlorophane solutions. Clinical evidence of toxicity was not observed, but it is felt that the value of hexachlorophane in topical steroid preparations should be re-appraised, particularly for long-term treatment of large eczematous areas.", "contents": "The absorption of hexachlorophane from Ultralanum ointment. The application of Ultralanum ointment to adult skin cases has produced levels of hexachlorophane in the plasma comparable with those found in infants who have undergone whole-body washing with hexachlorophane solutions. Clinical evidence of toxicity was not observed, but it is felt that the value of hexachlorophane in topical steroid preparations should be re-appraised, particularly for long-term treatment of large eczematous areas."}
{"id": "PMID:1100097", "title": "Effect of pindolol on intraocular pressure.", "content": "Pindolol, a strong beta-adrenergic blocking agent, instilled into the conjunctival sac of normal and glaucomatous eyes, produced a significant drop in intraocular pressure. This was not, at first, accompanied by any variation in outflow facility; only after prolonged treatment did an increase in facility appear, which accounted only for one-third of the tension-lowering effect. The drug was well tolerated, and did not affect either pupil motility or corneal sensitivity. It seems suitable for a trial use in the treatment of glaucoma.", "contents": "Effect of pindolol on intraocular pressure. Pindolol, a strong beta-adrenergic blocking agent, instilled into the conjunctival sac of normal and glaucomatous eyes, produced a significant drop in intraocular pressure. This was not, at first, accompanied by any variation in outflow facility; only after prolonged treatment did an increase in facility appear, which accounted only for one-third of the tension-lowering effect. The drug was well tolerated, and did not affect either pupil motility or corneal sensitivity. It seems suitable for a trial use in the treatment of glaucoma."}
{"id": "PMID:1100098", "title": "1H nuclear magnetic resonance of modified bases of valine transfer ribonucleic acid (Escherichia coli). A direct monitor of sequential thermal unfolding.", "content": "Proton magnetic resonances at 220 MHz from three nucleotide residues of valine I tRNA (Escherichia coli) serve as intrinsic probes of local molecular structure. Resonances from the methyl group of ribothymidine, the methyl group of N6-methyladenosine, and the C-5 methylene of dihydrouridine monitor separate conformational transitions in the TpsiC, anticodon, and dihydrouridine loops, respectively. As the temperature is raised in a solution containing 0.23 M Na+ and no Mg2+, the dihydrouridine region melts with a Tm of 55 degrees, the anticodon region at 58 degrees, and the TpsiC region at 67 degrees. The dihydrouridine nuclear magnetic resonance (NMR) transition correlates with the major change in absorbance monitored in the uv at 330 nm which is ascribed to structural pertubations near the 4-thiouracil moiety. On the NMR time scale slow exchange is seen throughout the temperature range for dihydrouridine and below the apparent Tm for the ribothymidine methyl group. Chemical shift and line width differences between folded and unfolded forms of the polynucleotide indicate that, in the native structure, ribothymidine is in a highly structured region and N6-methyladenosine is in a somewhat less restricted environment. Narrow line widths for the C-5 methylene triplet are found over the whole temperature range indicating that this base is undergoing rapid internal reorientation relative to the overall macromolecule.", "contents": "1H nuclear magnetic resonance of modified bases of valine transfer ribonucleic acid (Escherichia coli). A direct monitor of sequential thermal unfolding. Proton magnetic resonances at 220 MHz from three nucleotide residues of valine I tRNA (Escherichia coli) serve as intrinsic probes of local molecular structure. Resonances from the methyl group of ribothymidine, the methyl group of N6-methyladenosine, and the C-5 methylene of dihydrouridine monitor separate conformational transitions in the TpsiC, anticodon, and dihydrouridine loops, respectively. As the temperature is raised in a solution containing 0.23 M Na+ and no Mg2+, the dihydrouridine region melts with a Tm of 55 degrees, the anticodon region at 58 degrees, and the TpsiC region at 67 degrees. The dihydrouridine nuclear magnetic resonance (NMR) transition correlates with the major change in absorbance monitored in the uv at 330 nm which is ascribed to structural pertubations near the 4-thiouracil moiety. On the NMR time scale slow exchange is seen throughout the temperature range for dihydrouridine and below the apparent Tm for the ribothymidine methyl group. Chemical shift and line width differences between folded and unfolded forms of the polynucleotide indicate that, in the native structure, ribothymidine is in a highly structured region and N6-methyladenosine is in a somewhat less restricted environment. Narrow line widths for the C-5 methylene triplet are found over the whole temperature range indicating that this base is undergoing rapid internal reorientation relative to the overall macromolecule."}
{"id": "PMID:1100099", "title": "Fluorescence detected circular dichroism study of the anticodon loop of yeast tRNAPhe.", "content": "Fluorescence detected circular dichroism (FDCD) measurements have been used to study the conformations of the anticodon loop of yeast phenylalanine tRNA. To our knowledge this is the first application of fluorescence detected circular dichroism. Much smaller amounts of tRNA are needed for the measurement of FDCD than for the conventionally measured circular dichroism. Furthermore, FDCD is specific for conformational changes near the anticodon loop. The FDCD measurements suggest a transition in the anticodon loop near 20 degrees in 0.01 M MgCl2-0.1 M NaCl (pH 7). This is followed by a broad transition from 30 to 60 degrees and finally a sharp melting at 75 degrees consistent with the absorbance detected melting of the entire tRNA. Removal of Mg2+ from the tRNA at 1 degrees causes nearly a factor of two decrease in the FDCD near 230 nm. This indicates a decrease in conformational rigidity in the anticodon loop on removal of Mg2+.", "contents": "Fluorescence detected circular dichroism study of the anticodon loop of yeast tRNAPhe. Fluorescence detected circular dichroism (FDCD) measurements have been used to study the conformations of the anticodon loop of yeast phenylalanine tRNA. To our knowledge this is the first application of fluorescence detected circular dichroism. Much smaller amounts of tRNA are needed for the measurement of FDCD than for the conventionally measured circular dichroism. Furthermore, FDCD is specific for conformational changes near the anticodon loop. The FDCD measurements suggest a transition in the anticodon loop near 20 degrees in 0.01 M MgCl2-0.1 M NaCl (pH 7). This is followed by a broad transition from 30 to 60 degrees and finally a sharp melting at 75 degrees consistent with the absorbance detected melting of the entire tRNA. Removal of Mg2+ from the tRNA at 1 degrees causes nearly a factor of two decrease in the FDCD near 230 nm. This indicates a decrease in conformational rigidity in the anticodon loop on removal of Mg2+."}
{"id": "PMID:1100100", "title": "Interaction of actin with N-ethylmaleimide modified heavy meromyosin in the presence and absence of adenosine triphosphate.", "content": "N-Ethylmaleimide modified heavy meromyosin in only 3-fold activated by actin rather than 200-fold as is normal heavy meromyosin  (Silverman, R., Eisenberg, E., and Kielley, W. W. (1972), Nature (London) 240, 207). Ultracentrifuge studies demonstrated that in the absence of ATP the N-ethylmaleimide modified heavy meromyosin binds to actin at a ratio of 2 actins to 1 N-ethylmaleimide modified heavy meromyosin. However, it was found that most of the N-ethylmaleimide modified heavy meromyosin was not bound to actin during ATP hydrolysis. Ultracentrifuge studies demonstrated that in the presence of 25 or 50 mM KCl under conditions where the ATPase is maximally activated by actin, less than 5% of the N-ethylmaleimide modified heavy meromyosin was bound to actin. In the absence of KCl there was limited binding but even this binding did not appear to correlate with the N-ethylmaleimide modified heavy meromyosin ATPase rate. Turbidity and viscosity studies also indicated that in the presence of ATP under conditions of maximal actin activation the N-ethylmaleimide modified heavy meromyosin and actin are almost completely dissociated, whereas there is a marked increase in turbidity and viscosity after all of the ATP is hydrolyzed. These results suggest that in the presence of ATP and actin N-ethylmaleimide modified heavy meromyosin exists most of the time in a refractory state unable to bind to actin and only a small part of the time in a nonrefractory state which can interact with actin. It follows that the major rate-limiting step during actin activation is the transition from the refractory to the nonrefractory state. Since the actin activation of N-ethylmaleimide modified heavy meromyosin is lower than that of normal heavy meromyosin this transition may be slower for N-ethylmaleimide modified heavy meromyosin than for normal heavy meromyosin.", "contents": "Interaction of actin with N-ethylmaleimide modified heavy meromyosin in the presence and absence of adenosine triphosphate. N-Ethylmaleimide modified heavy meromyosin in only 3-fold activated by actin rather than 200-fold as is normal heavy meromyosin  (Silverman, R., Eisenberg, E., and Kielley, W. W. (1972), Nature (London) 240, 207). Ultracentrifuge studies demonstrated that in the absence of ATP the N-ethylmaleimide modified heavy meromyosin binds to actin at a ratio of 2 actins to 1 N-ethylmaleimide modified heavy meromyosin. However, it was found that most of the N-ethylmaleimide modified heavy meromyosin was not bound to actin during ATP hydrolysis. Ultracentrifuge studies demonstrated that in the presence of 25 or 50 mM KCl under conditions where the ATPase is maximally activated by actin, less than 5% of the N-ethylmaleimide modified heavy meromyosin was bound to actin. In the absence of KCl there was limited binding but even this binding did not appear to correlate with the N-ethylmaleimide modified heavy meromyosin ATPase rate. Turbidity and viscosity studies also indicated that in the presence of ATP under conditions of maximal actin activation the N-ethylmaleimide modified heavy meromyosin and actin are almost completely dissociated, whereas there is a marked increase in turbidity and viscosity after all of the ATP is hydrolyzed. These results suggest that in the presence of ATP and actin N-ethylmaleimide modified heavy meromyosin exists most of the time in a refractory state unable to bind to actin and only a small part of the time in a nonrefractory state which can interact with actin. It follows that the major rate-limiting step during actin activation is the transition from the refractory to the nonrefractory state. Since the actin activation of N-ethylmaleimide modified heavy meromyosin is lower than that of normal heavy meromyosin this transition may be slower for N-ethylmaleimide modified heavy meromyosin than for normal heavy meromyosin."}
{"id": "PMID:1100101", "title": "Reversible inactivation of vectorial phosphorylation by hydroxybutynoate in Escherichia coli membrane vesicles.", "content": "The acetylenic hydroxy acid 2-hydroxy-3-butynoate causes irreversible inactivation of the Escherichia coli membrane-bound flavoenzyme D-lactic dehyrogenase, and thus blocks D-lactate dependent active transport in isolated membrane vesicles [Walsh, C. T., Abeles, R. H., and Kaback H. R. (1972), J. Biol. Chem. 247, 7858]. The inactivator is a suicide substrate for the dehydrogenase, undergoing a small number of turnovers before partitioning between oxidation and inactiviation. It is now demonstrated that reactive product molecules of 2-keto-3-butynoate can diffuse in the membranes to a component of the phosphotransferase system and cause time-dependent and covalent inactivation of phosphoenolpyruvate-dependent hexose uptake. Membrane vesicles from double mutants with low levels of both D- and L-lactic dehydrogenase lose only 30 percent of their hexose uptake capacity on exposure to hydroxybutynoate under conditions sufficient to fully inactivate hexose transport in wild type vesicles. Transport of 1-[14C]hydroxybutynoate into vesicles is followed by rapid covalent labeling of membrane proteins by the reactive, enzymatically generating keto acid oxidation product. Incubation of hydroxybutynoate-inactivated vesicles (5% residual activity) for 20 min in buffer with 10 mM dithiothreitol results in reactivation of 63% of the hexose transport activity, a 12-fold increase in activity. No reactivation occurs if the vesicular phosphotransferase system is inactivated by keto acid derived from membrane oxidation of the olefinic congener 2-hydroxy-3-butenoate. In contrast to thiol reactivation of acetylenic-blocked glucose transport, blockage of D-lactate-stimulated proline uptake is not alleviated, stressing different modes of inactivation of the phosphotransferase system compared to the membranous lactate dehydrogenases.", "contents": "Reversible inactivation of vectorial phosphorylation by hydroxybutynoate in Escherichia coli membrane vesicles. The acetylenic hydroxy acid 2-hydroxy-3-butynoate causes irreversible inactivation of the Escherichia coli membrane-bound flavoenzyme D-lactic dehyrogenase, and thus blocks D-lactate dependent active transport in isolated membrane vesicles [Walsh, C. T., Abeles, R. H., and Kaback H. R. (1972), J. Biol. Chem. 247, 7858]. The inactivator is a suicide substrate for the dehydrogenase, undergoing a small number of turnovers before partitioning between oxidation and inactiviation. It is now demonstrated that reactive product molecules of 2-keto-3-butynoate can diffuse in the membranes to a component of the phosphotransferase system and cause time-dependent and covalent inactivation of phosphoenolpyruvate-dependent hexose uptake. Membrane vesicles from double mutants with low levels of both D- and L-lactic dehydrogenase lose only 30 percent of their hexose uptake capacity on exposure to hydroxybutynoate under conditions sufficient to fully inactivate hexose transport in wild type vesicles. Transport of 1-[14C]hydroxybutynoate into vesicles is followed by rapid covalent labeling of membrane proteins by the reactive, enzymatically generating keto acid oxidation product. Incubation of hydroxybutynoate-inactivated vesicles (5% residual activity) for 20 min in buffer with 10 mM dithiothreitol results in reactivation of 63% of the hexose transport activity, a 12-fold increase in activity. No reactivation occurs if the vesicular phosphotransferase system is inactivated by keto acid derived from membrane oxidation of the olefinic congener 2-hydroxy-3-butenoate. In contrast to thiol reactivation of acetylenic-blocked glucose transport, blockage of D-lactate-stimulated proline uptake is not alleviated, stressing different modes of inactivation of the phosphotransferase system compared to the membranous lactate dehydrogenases."}
{"id": "PMID:1100102", "title": "Limited trypsinolysis of native Escherichia coli elongation factor G.", "content": "Native elongation factor G possesses three peptide bonds or limited regions of the molecule which are especially sensitive to trypsinolysis. Cleavage at these sites occurs in sequence. Initially, the protein, a single peptide chain of 74,000 daltons, is rapidly split into a fragment of 71,000 daltons and one or more small peptides totaling 3000 daltons. This initial scission does not alter the gross three-dimentional structure of the protein, as the native and trypsin-cleaved protein have the same Kd on Sephadex gel filtration. Although cleaved, the modified elongation factor G retains full activity as measured by its ability to form complexes with the ribosome and guanine nucleotides. A single peptide bond (or region) in the 71,000-dalton fragment is then completely cleaved, yielding fragments of 47,000 and 29,000 daltons. These fragments do not remain associated under native conditions, as they are clearly resolved on gel filtration. Loss of activity is concomitant with the scission of the 71,000-dalton fragment. The third cleavage is complete under the conditions employed here and occurs in the 47,000-dalton peptide generating a fragment of 45,000 daltons and one or more small peptides totaling 2000 daltons. Prolonged treatment with higher levels of trypsin ultimately reduces the protein to small peptides but without generating further discrete fragments visible on sodium dodecyl sulfate gel electrophoresis.", "contents": "Limited trypsinolysis of native Escherichia coli elongation factor G. Native elongation factor G possesses three peptide bonds or limited regions of the molecule which are especially sensitive to trypsinolysis. Cleavage at these sites occurs in sequence. Initially, the protein, a single peptide chain of 74,000 daltons, is rapidly split into a fragment of 71,000 daltons and one or more small peptides totaling 3000 daltons. This initial scission does not alter the gross three-dimentional structure of the protein, as the native and trypsin-cleaved protein have the same Kd on Sephadex gel filtration. Although cleaved, the modified elongation factor G retains full activity as measured by its ability to form complexes with the ribosome and guanine nucleotides. A single peptide bond (or region) in the 71,000-dalton fragment is then completely cleaved, yielding fragments of 47,000 and 29,000 daltons. These fragments do not remain associated under native conditions, as they are clearly resolved on gel filtration. Loss of activity is concomitant with the scission of the 71,000-dalton fragment. The third cleavage is complete under the conditions employed here and occurs in the 47,000-dalton peptide generating a fragment of 45,000 daltons and one or more small peptides totaling 2000 daltons. Prolonged treatment with higher levels of trypsin ultimately reduces the protein to small peptides but without generating further discrete fragments visible on sodium dodecyl sulfate gel electrophoresis."}
{"id": "PMID:1100103", "title": "Raman spectra and structure of yeast phenylalanine transfer RNA in the crystalline state and in solution.", "content": "Laser Raman spectra of yeast phenylalanine transfer RNA have been obtained in solution and in orthorhombic and hexagonal crystals. So far as one can tell from the spectra, which are identical in the two crystal forms, the molecular structure of the tRNA is not altered by differences in molecular packing in these two unit cells. In addition, the spectra of the two crystal forms show the same characteristic Raman frequencies and intensities as those of the tRNA in aqueous solution. Thus the structure of the tRNA molecule appears to be the same in the crystals and in aqueous solution. From the spectroscopic changes that result when Mg2+ ions are removed from the native tRNA, it is concluded that the removal of Mg2+ produces a partial disordering of the ribophosphate backbone of the molecule and a lowering of its melting temperature. The melting is shown to be a complex process in that the vibrations specific for adenine indicate a slightly lower melting temperature and those specific for guanine a slightly higher melting temperature than that of the ribophosphate backbone.", "contents": "Raman spectra and structure of yeast phenylalanine transfer RNA in the crystalline state and in solution. Laser Raman spectra of yeast phenylalanine transfer RNA have been obtained in solution and in orthorhombic and hexagonal crystals. So far as one can tell from the spectra, which are identical in the two crystal forms, the molecular structure of the tRNA is not altered by differences in molecular packing in these two unit cells. In addition, the spectra of the two crystal forms show the same characteristic Raman frequencies and intensities as those of the tRNA in aqueous solution. Thus the structure of the tRNA molecule appears to be the same in the crystals and in aqueous solution. From the spectroscopic changes that result when Mg2+ ions are removed from the native tRNA, it is concluded that the removal of Mg2+ produces a partial disordering of the ribophosphate backbone of the molecule and a lowering of its melting temperature. The melting is shown to be a complex process in that the vibrations specific for adenine indicate a slightly lower melting temperature and those specific for guanine a slightly higher melting temperature than that of the ribophosphate backbone."}
{"id": "PMID:1100104", "title": "Escherichia coli stringent factor binds to ribosomes at a site different from that of elongation factor Tu or G.", "content": "The binding of Escherichia coli stringent factor to ribosomes has been studied; the reaction depends on 50S and 30S ribosomal subunits and poly(U) as messenger RNA. The ribosome-stringent factor complex is formed at 5-10 mM magnesium acetate; NH4 ions are inhibitory. Binding of the stringent factor to the 70S-mRNA complex is not stimulated by uncharged tRNA. The ribosomal binding site(s) for the stringent factor does not overlap with the one known for the elongation factor Tu (EF-Tu) or G (EF-G). Ribosomes carrying either EF-Tu or EF-G are active in binding the stringent factor; however, they are inactive in synthesizing guanosine 5'-triphosphate 3'-diphosphate (pppGpp) and guanosine 5'-diphosphate 3'-diphosphate (ppGpp). The latter result is due to the blockage of the ribosomal acceptor site by the aminoacyl-tRNA and/or elongation factors. That stringent and elongation factors do not compete for identical ribosomal region(s) is supported by: (1) the reverse experiments where ribosomes charged with the stringent factor are fully active in EF-Tu or EF-G dependent functions; (2) ribosomes that lack the two ribosomal proteins L7 and L12 known to be essential for EF-Tu and EF-G functions bind the stringent factor and are active in synthesizing pppGpp and ppGpp.", "contents": "Escherichia coli stringent factor binds to ribosomes at a site different from that of elongation factor Tu or G. The binding of Escherichia coli stringent factor to ribosomes has been studied; the reaction depends on 50S and 30S ribosomal subunits and poly(U) as messenger RNA. The ribosome-stringent factor complex is formed at 5-10 mM magnesium acetate; NH4 ions are inhibitory. Binding of the stringent factor to the 70S-mRNA complex is not stimulated by uncharged tRNA. The ribosomal binding site(s) for the stringent factor does not overlap with the one known for the elongation factor Tu (EF-Tu) or G (EF-G). Ribosomes carrying either EF-Tu or EF-G are active in binding the stringent factor; however, they are inactive in synthesizing guanosine 5'-triphosphate 3'-diphosphate (pppGpp) and guanosine 5'-diphosphate 3'-diphosphate (ppGpp). The latter result is due to the blockage of the ribosomal acceptor site by the aminoacyl-tRNA and/or elongation factors. That stringent and elongation factors do not compete for identical ribosomal region(s) is supported by: (1) the reverse experiments where ribosomes charged with the stringent factor are fully active in EF-Tu or EF-G dependent functions; (2) ribosomes that lack the two ribosomal proteins L7 and L12 known to be essential for EF-Tu and EF-G functions bind the stringent factor and are active in synthesizing pppGpp and ppGpp."}
{"id": "PMID:1100105", "title": "Cobalt(III) labeled aspartokinase-homoserine dehydrogenase of Escherichia coli.", "content": "The kinase activity of the threonine-sensitive aspartokinase-homoserine dehydrogenase enzyme complex of Escherichia coli was selectively inactivated by Co(III) incorporation. Incubation of the enzyme with Co(II) in the presence of oxygen or H2O2 resulted in incorporation of one Co(III) per subunit. The cobalt(III) bound to the enzyme was not removable by dialysis and presumably results from formation of \"inert\" coordination complexes with ligands contributed by the enzyme. Cobalt was released from the enzyme by incubation with dithiothreitol but not by metal chelating agents. The Co(III)-labeled enzyme was aspartokinase inactive but still retained 60% of its original homoserine dehydrogenase activity. Studies of the time course of inactivation showed aspartokinase inactivation paralleled Co(III) incorporation. The residual dehydrogenase activity of aspartokinase inactive enzyme was still inhibited by threonine Thus, Co(III) incorporation seems to result in a specific inactivation of kinase activity which permits enumeration of the number of aspartokinase sites. Limited alpha-chymotrypsin digestion of Co(III)-enzyme produced homoserine dehydrogenase-active fragments devoid of Co(III), further confirming the specificity of the labeling procedure. Aspartokinase inactivation obtained without concomitant desensitization of homoserine dehydrogenase to threonine inhibition suggests that kinase active site integrity is not required for threonine binding and inhibition of homoserine dehydrogenase.", "contents": "Cobalt(III) labeled aspartokinase-homoserine dehydrogenase of Escherichia coli. The kinase activity of the threonine-sensitive aspartokinase-homoserine dehydrogenase enzyme complex of Escherichia coli was selectively inactivated by Co(III) incorporation. Incubation of the enzyme with Co(II) in the presence of oxygen or H2O2 resulted in incorporation of one Co(III) per subunit. The cobalt(III) bound to the enzyme was not removable by dialysis and presumably results from formation of \"inert\" coordination complexes with ligands contributed by the enzyme. Cobalt was released from the enzyme by incubation with dithiothreitol but not by metal chelating agents. The Co(III)-labeled enzyme was aspartokinase inactive but still retained 60% of its original homoserine dehydrogenase activity. Studies of the time course of inactivation showed aspartokinase inactivation paralleled Co(III) incorporation. The residual dehydrogenase activity of aspartokinase inactive enzyme was still inhibited by threonine Thus, Co(III) incorporation seems to result in a specific inactivation of kinase activity which permits enumeration of the number of aspartokinase sites. Limited alpha-chymotrypsin digestion of Co(III)-enzyme produced homoserine dehydrogenase-active fragments devoid of Co(III), further confirming the specificity of the labeling procedure. Aspartokinase inactivation obtained without concomitant desensitization of homoserine dehydrogenase to threonine inhibition suggests that kinase active site integrity is not required for threonine binding and inhibition of homoserine dehydrogenase."}
{"id": "PMID:1100106", "title": "Fragments of bovine serum albumin produced by limited proteolysis. Isolation and characterization of peptic fragments.", "content": "Five new fragments of bovine serum albumin have been isolated following limited peptic hydrolysis. These fragments, and the two peptic fragments previously described by King (King, T.P. (1973), Arch. Biochem. Biophys. 156, 509), were positioned within the albumin sequence published by Brown (Brown, J.R. (1975), Fed. Proc., Fed. Am. Soc. Exp. Biol. 34, 591) on the basis of molecular weight, amino acid composition, and amino- and carboxyl-terminal sequences. The fragments correspond to residues 1-385, 1-306, 307-581, 49-185, 186-306, 307-385, and 353-503 in the albumin sequence. These peptides are likely to be native in structure since disulfide bonds were not cleaved during their preparation. In each case the amino acid composition and terminal sequences have confirmed the bovine serum albumin sequence and disulfide bridging pattern proposed by Brown, and the offer further proof that bovine albumin is composed of a series of nine independent loops. These fragments should be useful in elucidating the structure-function relationships of albumin.", "contents": "Fragments of bovine serum albumin produced by limited proteolysis. Isolation and characterization of peptic fragments. Five new fragments of bovine serum albumin have been isolated following limited peptic hydrolysis. These fragments, and the two peptic fragments previously described by King (King, T.P. (1973), Arch. Biochem. Biophys. 156, 509), were positioned within the albumin sequence published by Brown (Brown, J.R. (1975), Fed. Proc., Fed. Am. Soc. Exp. Biol. 34, 591) on the basis of molecular weight, amino acid composition, and amino- and carboxyl-terminal sequences. The fragments correspond to residues 1-385, 1-306, 307-581, 49-185, 186-306, 307-385, and 353-503 in the albumin sequence. These peptides are likely to be native in structure since disulfide bonds were not cleaved during their preparation. In each case the amino acid composition and terminal sequences have confirmed the bovine serum albumin sequence and disulfide bridging pattern proposed by Brown, and the offer further proof that bovine albumin is composed of a series of nine independent loops. These fragments should be useful in elucidating the structure-function relationships of albumin."}
{"id": "PMID:1100107", "title": "DNA-binding chromosomal non-histone proteins. Isolation, characterization, and tissue specifity.", "content": "A fractionation schedule is described which allows the isolation of a group of chromosomal non-histone proteins (NP) with affinity for DNA. In polyacrylamide gel electrophoresis these proteins isolated from rat liver are represented principally by a group of low molecular weight polypeptides. The NP fraction comprises about 2-4% of the total chromatin protein content in rat liver or Novikoff hepatoma. Experiments in vivo and in vitro revealed that the NP proteins do not incorporate significant amounts of 32P. Complexes of the chromosomal proteins NP with homologous DNA are immunologically tissue specific and the specificity can be transferred by reconstituting the NP proteins from one tissue to the residual chromatin from another.", "contents": "DNA-binding chromosomal non-histone proteins. Isolation, characterization, and tissue specifity. A fractionation schedule is described which allows the isolation of a group of chromosomal non-histone proteins (NP) with affinity for DNA. In polyacrylamide gel electrophoresis these proteins isolated from rat liver are represented principally by a group of low molecular weight polypeptides. The NP fraction comprises about 2-4% of the total chromatin protein content in rat liver or Novikoff hepatoma. Experiments in vivo and in vitro revealed that the NP proteins do not incorporate significant amounts of 32P. Complexes of the chromosomal proteins NP with homologous DNA are immunologically tissue specific and the specificity can be transferred by reconstituting the NP proteins from one tissue to the residual chromatin from another."}
{"id": "PMID:1100108", "title": "Phospholipid metabolism of stimulated lymphocytes. Composition of phospholipid fatty acids.", "content": "Lymph node lymphocytes and thymocytes from different species were isolated. Rabbit and calf thymocytes were stimulated in vitro with concanavalin A. Phospholipid fatty acids of these cells were analyzed and their positional distribution was determined. When compared with liver, phosphatidylcholine of unstimulated lymphocytes was found to contain relatively high amounts of palmitic acid in position 2 and oleic acid in position 1. After stimulation of rabbit thymus cells, the content of polyunsaturated fatty acids (linoleic and arachidonic acid) increased. Thus the ratio of polyenoic acids (18:2 + 20:4) to saturated fatty acids was doubled when compared to control cells. Similar results were obtained after in vivo stimualtion with Mycobacterium Calmette Guerin. The correlation of these findings with the activation of acyl-CoA:lysolecithin acyltransferase, and their relevance for changes of membrane fluidity during lymphocyte stimulation is discussed.", "contents": "Phospholipid metabolism of stimulated lymphocytes. Composition of phospholipid fatty acids. Lymph node lymphocytes and thymocytes from different species were isolated. Rabbit and calf thymocytes were stimulated in vitro with concanavalin A. Phospholipid fatty acids of these cells were analyzed and their positional distribution was determined. When compared with liver, phosphatidylcholine of unstimulated lymphocytes was found to contain relatively high amounts of palmitic acid in position 2 and oleic acid in position 1. After stimulation of rabbit thymus cells, the content of polyunsaturated fatty acids (linoleic and arachidonic acid) increased. Thus the ratio of polyenoic acids (18:2 + 20:4) to saturated fatty acids was doubled when compared to control cells. Similar results were obtained after in vivo stimualtion with Mycobacterium Calmette Guerin. The correlation of these findings with the activation of acyl-CoA:lysolecithin acyltransferase, and their relevance for changes of membrane fluidity during lymphocyte stimulation is discussed."}
{"id": "PMID:1100109", "title": "Formation of lipid-bound oligosaccharides in yeast.", "content": "Incubation of a membrane fraction from Saccharomyces cerevisiae with UDP-N-acetyl [14C]glucosamine catalyzes the transfer of N-acetylglucosamine to an endogenous lipid fraction as well as a methanol-insoluble polymer. The glycolipid was shown to separate into three compounds by thin-layer chromatography. The biosynthesis of two of them could clearly be stimuated by the addition of dolichol monophosphate to the incubation mixture. Evidence is presented that the substances are dolichol pyrophosphate derivatives: dolichol pyrophosphate N-acetylglucosamine and dolichol pyrophosphate di-N-acetylchitobiose. The formation of the chitobiose-containing lipid was increased by reincubation of the glycolipid with non-radioactive UDP-N-acetylglucosamine. The same particulate preparation transferred mannose from GDPmannose to dolichol pyrophosphate di-N-acetylchitobiose, giving rise to a lipid-bound oligosaccharide. Molecular weight determination of the oligosaccharide moiety gave a value of 780, which is consistent with a tetrasaccharide containing two mannose subunits attached to di-N-acetylchitobiose. The methanol-insoluble radioactive product obtained in the presence of UDP-N-acetyl[14C]glucosamine was transformed by pronase treatment to a large extent into dialyzable material. It is suggested that the glycolipids described serve as intermediates in the glycosylation of yeast mannoproteins.", "contents": "Formation of lipid-bound oligosaccharides in yeast. Incubation of a membrane fraction from Saccharomyces cerevisiae with UDP-N-acetyl [14C]glucosamine catalyzes the transfer of N-acetylglucosamine to an endogenous lipid fraction as well as a methanol-insoluble polymer. The glycolipid was shown to separate into three compounds by thin-layer chromatography. The biosynthesis of two of them could clearly be stimuated by the addition of dolichol monophosphate to the incubation mixture. Evidence is presented that the substances are dolichol pyrophosphate derivatives: dolichol pyrophosphate N-acetylglucosamine and dolichol pyrophosphate di-N-acetylchitobiose. The formation of the chitobiose-containing lipid was increased by reincubation of the glycolipid with non-radioactive UDP-N-acetylglucosamine. The same particulate preparation transferred mannose from GDPmannose to dolichol pyrophosphate di-N-acetylchitobiose, giving rise to a lipid-bound oligosaccharide. Molecular weight determination of the oligosaccharide moiety gave a value of 780, which is consistent with a tetrasaccharide containing two mannose subunits attached to di-N-acetylchitobiose. The methanol-insoluble radioactive product obtained in the presence of UDP-N-acetyl[14C]glucosamine was transformed by pronase treatment to a large extent into dialyzable material. It is suggested that the glycolipids described serve as intermediates in the glycosylation of yeast mannoproteins."}
{"id": "PMID:1100110", "title": "Inhibition of thiamine transport in anaerobic baker's yeast by iodoacetate, 2,4-dinitrophenol N,N'-dicyclohexylcarbodiimide and fatty acids.", "content": "1. [14C]Thiamine uptake by baker's yeast (Saccharomyces cerevisiae) was strongly inhibited by 0.2 mM iodoacetate, 0.2 mM 2,4-dinitrophenol and 0.1 mM N,N'-dicyclohexylcarbodiimide under anaerobic conditions. 2. The inhibition of anaerobic [14C]thiamine uptake by these inhibitors was accompanied by almost parallel decreases in the ATP level of the yeast cells. 3. On the other hand, the short-chain fatty acids inhibited [14C]thiamine uptake to a large extent, without greatly affecting the intracellular ATP level. This suggests that the acids primarily block the use of energy from ATP for the transport rather than the fermentation process. 4. Caproate, which has a most pronounced inhibitory effect on [14C]thiamine uptake, significantly prevented the dissipation of an energized membrane state of yeast cells necessary for the active transport of thiamine. 5. Possible ways in which the inhibitors may affect thiamine uptake were discussed.", "contents": "Inhibition of thiamine transport in anaerobic baker's yeast by iodoacetate, 2,4-dinitrophenol N,N'-dicyclohexylcarbodiimide and fatty acids. 1. [14C]Thiamine uptake by baker's yeast (Saccharomyces cerevisiae) was strongly inhibited by 0.2 mM iodoacetate, 0.2 mM 2,4-dinitrophenol and 0.1 mM N,N'-dicyclohexylcarbodiimide under anaerobic conditions. 2. The inhibition of anaerobic [14C]thiamine uptake by these inhibitors was accompanied by almost parallel decreases in the ATP level of the yeast cells. 3. On the other hand, the short-chain fatty acids inhibited [14C]thiamine uptake to a large extent, without greatly affecting the intracellular ATP level. This suggests that the acids primarily block the use of energy from ATP for the transport rather than the fermentation process. 4. Caproate, which has a most pronounced inhibitory effect on [14C]thiamine uptake, significantly prevented the dissipation of an energized membrane state of yeast cells necessary for the active transport of thiamine. 5. Possible ways in which the inhibitors may affect thiamine uptake were discussed."}
{"id": "PMID:1100111", "title": "The effects of D- and L-glyceraldehyde on glucose oxidation, insulin secretion and insulin biosynthesis by pancreatic islets of the rat.", "content": "D-glyceraldehyde stimulated insulin secretion from isolated rat pancreatic islets in static incubation and perifusion systems. At low concentrations (2-4 mM) D-glyceraldehyde was a more potent secretagogue than glucose. The insulinotropic action of 15 mM D-glyceraldehyde was not affected by D-mannoheptulose, was potentiated by cytochalasin B (5 mug/ml) and theophylline (4 mM), and was inhibited by both adrenalin (2 muM) and somatostatin (10 mug/ml). D-glyceraldehyde at a concentration of 1.5 mM produced a 10-fold increase of L-[4,5-3H]leucine incorporation into proinsulin and insulin without a significant increase into other islet proteins. Glucose at 1.5 mM did not stimulate proinsulin biosynthesis. D-Glyceraldehyde at concentrations higher than 1.5 mM, in marked contrast to glucose, progressively inhibited incorporation of labelled leucine into proinsulin + insulin and other islet proteins. D-Glyceraldehyde also inhibited the oxidation of glucose. L-Glyceraldehyde did not stimulate proinsulin biosynthesis and had less effect than the D-isomer on insulin release and glucose oxidation. The results strongly suggest that metabolites below D-glyceraldehyde-3-P are signals for insulin biosynthesis and release. Interaction of D-glyceraldehyde with a \"membrane receptor\" cannot, however, be excluded with certainty.", "contents": "The effects of D- and L-glyceraldehyde on glucose oxidation, insulin secretion and insulin biosynthesis by pancreatic islets of the rat. D-glyceraldehyde stimulated insulin secretion from isolated rat pancreatic islets in static incubation and perifusion systems. At low concentrations (2-4 mM) D-glyceraldehyde was a more potent secretagogue than glucose. The insulinotropic action of 15 mM D-glyceraldehyde was not affected by D-mannoheptulose, was potentiated by cytochalasin B (5 mug/ml) and theophylline (4 mM), and was inhibited by both adrenalin (2 muM) and somatostatin (10 mug/ml). D-glyceraldehyde at a concentration of 1.5 mM produced a 10-fold increase of L-[4,5-3H]leucine incorporation into proinsulin and insulin without a significant increase into other islet proteins. Glucose at 1.5 mM did not stimulate proinsulin biosynthesis. D-Glyceraldehyde at concentrations higher than 1.5 mM, in marked contrast to glucose, progressively inhibited incorporation of labelled leucine into proinsulin + insulin and other islet proteins. D-Glyceraldehyde also inhibited the oxidation of glucose. L-Glyceraldehyde did not stimulate proinsulin biosynthesis and had less effect than the D-isomer on insulin release and glucose oxidation. The results strongly suggest that metabolites below D-glyceraldehyde-3-P are signals for insulin biosynthesis and release. Interaction of D-glyceraldehyde with a \"membrane receptor\" cannot, however, be excluded with certainty."}
{"id": "PMID:1100112", "title": "Glucosamine-labelled envelope proteins of Escherichia coli K-12. I. Electrophoretic studies and partial fractionation of phenol-soluble proteins.", "content": "Hydrophobic envelope proteins were extracted by phenol from a glucosamine- and leucine-requiring mutant of Escherichia coli K-12 (E-110). Three protein fractions labelled with D-[1-14C]glucosamine and L-[4,5-3H]leucine were obtained by electrophoretic separation. Envelopes were isolated from cells labelled with D-[1-14C]glucosamine--HCL and acid hydrolyzed. At least 68% of the radioactivity was recovered as glucosamine and glucose with no random distribution of label. Fingerprinting of pronase digests of glucosamine-labelled proteins showed four radioactive spots associated with peptides. The glycoproteins were pronase- and trypsin-sensitive and had apparent molecular weights of 11 000 (fast mobility), 35 000 (intermediate mobility) and 62 000 (slow mobility) as estimated by sodium dodecyl sulfate-polyacrylamide disc electrophoresis. The two heavier fractions were labelled with meso-diamino[1,7-14C2]pimelic acid, while orth[32P]phosphate was not incorporated into any fraction. The glucosamine radioactivity of the fast fraction underwent rapid changes upon a chase with non-radioactive glucosamine. Using a Sephadex LH-20 column, the radioactive proteins were separated from the phenol and subsequently fractionated on a DEAE-cellulose column. The DEAE-cellulose fractions were distinct from each other in the number and composition of protein bands, when analyzed by sodium dodecyl sulfate-polyacrylamide disc electrophoresis. Radioactive bands with intermediate and fast electrophoretic mobilities were found in separate DEAE-cellulose fractions.", "contents": "Glucosamine-labelled envelope proteins of Escherichia coli K-12. I. Electrophoretic studies and partial fractionation of phenol-soluble proteins. Hydrophobic envelope proteins were extracted by phenol from a glucosamine- and leucine-requiring mutant of Escherichia coli K-12 (E-110). Three protein fractions labelled with D-[1-14C]glucosamine and L-[4,5-3H]leucine were obtained by electrophoretic separation. Envelopes were isolated from cells labelled with D-[1-14C]glucosamine--HCL and acid hydrolyzed. At least 68% of the radioactivity was recovered as glucosamine and glucose with no random distribution of label. Fingerprinting of pronase digests of glucosamine-labelled proteins showed four radioactive spots associated with peptides. The glycoproteins were pronase- and trypsin-sensitive and had apparent molecular weights of 11 000 (fast mobility), 35 000 (intermediate mobility) and 62 000 (slow mobility) as estimated by sodium dodecyl sulfate-polyacrylamide disc electrophoresis. The two heavier fractions were labelled with meso-diamino[1,7-14C2]pimelic acid, while orth[32P]phosphate was not incorporated into any fraction. The glucosamine radioactivity of the fast fraction underwent rapid changes upon a chase with non-radioactive glucosamine. Using a Sephadex LH-20 column, the radioactive proteins were separated from the phenol and subsequently fractionated on a DEAE-cellulose column. The DEAE-cellulose fractions were distinct from each other in the number and composition of protein bands, when analyzed by sodium dodecyl sulfate-polyacrylamide disc electrophoresis. Radioactive bands with intermediate and fast electrophoretic mobilities were found in separate DEAE-cellulose fractions."}
{"id": "PMID:1100113", "title": "Glucosamine-labelled envelope proteins of Escherichia coli K-12. II. Location in inner and outer membranes.", "content": "Outer and inner membranes were prepared from a culture of a glucosamine- and leucine-requiring mutant of Escherichia coli K-12, which was grown on L-[4,5-(3)H2]leucine and D-[1-(14)C]glucosamine and \"chased\" with unlabelled medium. Phenol-soluble glycoproteins were obtained from these membranes after phospholipid extraction. Sodium dodecyl sulfate-disc electrophoresis of the outer-membrane glycoproteins separated two components which were labelled with both isotopes and had a fast (F) and intermediate (I) migration. Dodecyl sulfate electrophoresis of the inner-membrane glycoproteins showed that only the F band incorporated both 14C and 3H labels. The 3H to 14C ratio in the F band from the outer membrane was completely different from the isotopic ratio in the F band from the inner membrane, indicating that these components were not identical despite their similar molecular weights. The F bands from both membranes lost their label during the chase with unlabelled medium, while the I band remained relatively stable. Chloroform-soluble 14C label extracted from the outer membrane decreased during the chase. In contrast, the chloroform-soluble 14C from the inner membrane, accumulated during the chase.", "contents": "Glucosamine-labelled envelope proteins of Escherichia coli K-12. II. Location in inner and outer membranes. Outer and inner membranes were prepared from a culture of a glucosamine- and leucine-requiring mutant of Escherichia coli K-12, which was grown on L-[4,5-(3)H2]leucine and D-[1-(14)C]glucosamine and \"chased\" with unlabelled medium. Phenol-soluble glycoproteins were obtained from these membranes after phospholipid extraction. Sodium dodecyl sulfate-disc electrophoresis of the outer-membrane glycoproteins separated two components which were labelled with both isotopes and had a fast (F) and intermediate (I) migration. Dodecyl sulfate electrophoresis of the inner-membrane glycoproteins showed that only the F band incorporated both 14C and 3H labels. The 3H to 14C ratio in the F band from the outer membrane was completely different from the isotopic ratio in the F band from the inner membrane, indicating that these components were not identical despite their similar molecular weights. The F bands from both membranes lost their label during the chase with unlabelled medium, while the I band remained relatively stable. Chloroform-soluble 14C label extracted from the outer membrane decreased during the chase. In contrast, the chloroform-soluble 14C from the inner membrane, accumulated during the chase."}
{"id": "PMID:1100114", "title": "Effect of activated nitrofurans on DNA.", "content": "Enzymically activated nitrofurazone reacts with co-valently closed circular DNA (derived from Escherichia coli minicells carrying lambdadv) to give at least two kinds of damage: breaks which are detected on neutral sucrose gradients and alkali-labile lesions in DNA which are converted to breaks when the DNA is subsequently Treated with alkali. DNA, isolated from nimicells exposed to the drug, also contains lesions which are converted to breaks upon treatment with endonuclease preparations obtained from Micrococcus luteus. Minicells repaired both breaks and nuclease-susceptible lesions within 2 h but did not repair alkali labile lesions within that time. Experiments with three other nitrofurans show that there are considerable differences in the degree to which DNA is damaged by activated metabolites of various derivatives and that the potency of the compounds as mutagens and carcinogens is correlated with the amount of damage caused to minicell DNA.", "contents": "Effect of activated nitrofurans on DNA. Enzymically activated nitrofurazone reacts with co-valently closed circular DNA (derived from Escherichia coli minicells carrying lambdadv) to give at least two kinds of damage: breaks which are detected on neutral sucrose gradients and alkali-labile lesions in DNA which are converted to breaks when the DNA is subsequently Treated with alkali. DNA, isolated from nimicells exposed to the drug, also contains lesions which are converted to breaks upon treatment with endonuclease preparations obtained from Micrococcus luteus. Minicells repaired both breaks and nuclease-susceptible lesions within 2 h but did not repair alkali labile lesions within that time. Experiments with three other nitrofurans show that there are considerable differences in the degree to which DNA is damaged by activated metabolites of various derivatives and that the potency of the compounds as mutagens and carcinogens is correlated with the amount of damage caused to minicell DNA."}
{"id": "PMID:1100115", "title": "Temperature and salt effects on the formation of preinitiation complexes between RNA polymerase and phage DNA.", "content": "The influence of temperature and KCl concentration on the formation of rifampicin-resistant preinitiation complexes by holo RNA polymerase has been compared for T4 DNA and Azotobacter phage A21 DNA. The sharp transition with respect to temperature between an inactive complex of polymerase and DNA and a preinitiation complex reflects an equilibrium between the two complexes, the position of which depends on the temperature and the salt concentration. The transition is shifted to higher temperatures by increasing the KCl concentration. The position of this transition is characteristically different for T4 and A21 DNA. The midpoint for A21 DNA is about 15 degrees C above that for T4 at 0.006 M KCl. At 0.15 M KCl the transition for A21 DNA cannot be observed below 37 degrees C. This difference is responsible for the apparent inhibition of a21 dna transcription by KCl and for the low template activity of A21 DNA under the conditions of the standard assay. Both holo and core RNA polymerases are able to form complexes with A21 DNA that are resistant to attack by rifampicin. The second-order rate constant for the inactivation  of the complex with the core enxyme is three times greater than that for the complex with the holoenzyme.", "contents": "Temperature and salt effects on the formation of preinitiation complexes between RNA polymerase and phage DNA. The influence of temperature and KCl concentration on the formation of rifampicin-resistant preinitiation complexes by holo RNA polymerase has been compared for T4 DNA and Azotobacter phage A21 DNA. The sharp transition with respect to temperature between an inactive complex of polymerase and DNA and a preinitiation complex reflects an equilibrium between the two complexes, the position of which depends on the temperature and the salt concentration. The transition is shifted to higher temperatures by increasing the KCl concentration. The position of this transition is characteristically different for T4 and A21 DNA. The midpoint for A21 DNA is about 15 degrees C above that for T4 at 0.006 M KCl. At 0.15 M KCl the transition for A21 DNA cannot be observed below 37 degrees C. This difference is responsible for the apparent inhibition of a21 dna transcription by KCl and for the low template activity of A21 DNA under the conditions of the standard assay. Both holo and core RNA polymerases are able to form complexes with A21 DNA that are resistant to attack by rifampicin. The second-order rate constant for the inactivation  of the complex with the core enxyme is three times greater than that for the complex with the holoenzyme."}
{"id": "PMID:1100117", "title": "Ribosome run through of the termination codon in the absence of the ribosome releasing factor.", "content": "The ribosome releasing factor (RR factor) which releases ribosomes from mRNA at the termination codon has been examined for its effects on the amino acid incorporation programmed by wild type R17 Phage RNA and amB2 R17 RNA. When RR factor was added at the beginning of the incorporation, there was no effect on the initial rate of incorporation but it reduced the final level of incorporation. The reduction of the final level of incorporation was more pronounced for histidine incorporation than for valine incorporation suggesting that the translation of the RNA polymerase cistron was more influenced by RR factor. These experiments were carried out under conditions where no reinitiation of protein synthesis occurred. In the presence of RR factor, suppressor tRNA functioned better for the incorporation of amino acids into proteins with amB2 R17 RNA than did wild type tRNA. No such differential effect of suppressor tRNA was observed in the absence of RR factor. This suggests that the ribosome has to be released from mRNA by RR factor in order for the amber mutation to be effective.", "contents": "Ribosome run through of the termination codon in the absence of the ribosome releasing factor. The ribosome releasing factor (RR factor) which releases ribosomes from mRNA at the termination codon has been examined for its effects on the amino acid incorporation programmed by wild type R17 Phage RNA and amB2 R17 RNA. When RR factor was added at the beginning of the incorporation, there was no effect on the initial rate of incorporation but it reduced the final level of incorporation. The reduction of the final level of incorporation was more pronounced for histidine incorporation than for valine incorporation suggesting that the translation of the RNA polymerase cistron was more influenced by RR factor. These experiments were carried out under conditions where no reinitiation of protein synthesis occurred. In the presence of RR factor, suppressor tRNA functioned better for the incorporation of amino acids into proteins with amB2 R17 RNA than did wild type tRNA. No such differential effect of suppressor tRNA was observed in the absence of RR factor. This suggests that the ribosome has to be released from mRNA by RR factor in order for the amber mutation to be effective."}
{"id": "PMID:1100118", "title": "DNA synthesis in vitro in lysates of Escherichia coli.", "content": "A system using crude lysates of Escherichia coli PolA- for in vitro synthesis of DNA has properties similar to those described previously for washed DNA - membrane complexes, i.e. short-lived synthesis of DNA, 2-3-fold stimulation by ATP, semiconservative form of synthesis, and most of the product in the size distribution of Okazaki fragments. The proximate substrate is shown to be the deoxynucleoside triphosphate. The lysate system can be fractionated into a particular membrane - DNA complex and a soluble portion, each of which is inactive by itself, but which upon mixing restores activity. The particulate fraction provides template as well as essential protein(s). The soluble fraction contains DNA polymerases II and III both of which are capable of the necessary polymerase function, and a factor that is required for the stimulation of DNA synthesis by ATP. The stimulatory activity can also be assayed by restoration of activity to an aged lysate, which has lost the ATP-dependent synthesis; the activity is not a kinase, and is not an ATP-dependent nuclease, at least of the type currently known. The ATP-dependent stimulatory factor has been partially purified but further purification or characterization of it has been limited by its extreme instability. Both the stimulatory factor and ATP are required for semiconservative synthesis in the lysate, and for synthesis of the short fragments. Similar to results in vivo and in permeable cells the new DNA appears to contain RNA, as judged by the evidence for RNA - DNA junctions from modified nearest neighbor experiments. However, the nucleotides at the RNA - DNA junctions in the lysate system are not specific, in contrast to results in permeabilized Escherichia coli.", "contents": "DNA synthesis in vitro in lysates of Escherichia coli. A system using crude lysates of Escherichia coli PolA- for in vitro synthesis of DNA has properties similar to those described previously for washed DNA - membrane complexes, i.e. short-lived synthesis of DNA, 2-3-fold stimulation by ATP, semiconservative form of synthesis, and most of the product in the size distribution of Okazaki fragments. The proximate substrate is shown to be the deoxynucleoside triphosphate. The lysate system can be fractionated into a particular membrane - DNA complex and a soluble portion, each of which is inactive by itself, but which upon mixing restores activity. The particulate fraction provides template as well as essential protein(s). The soluble fraction contains DNA polymerases II and III both of which are capable of the necessary polymerase function, and a factor that is required for the stimulation of DNA synthesis by ATP. The stimulatory activity can also be assayed by restoration of activity to an aged lysate, which has lost the ATP-dependent synthesis; the activity is not a kinase, and is not an ATP-dependent nuclease, at least of the type currently known. The ATP-dependent stimulatory factor has been partially purified but further purification or characterization of it has been limited by its extreme instability. Both the stimulatory factor and ATP are required for semiconservative synthesis in the lysate, and for synthesis of the short fragments. Similar to results in vivo and in permeable cells the new DNA appears to contain RNA, as judged by the evidence for RNA - DNA junctions from modified nearest neighbor experiments. However, the nucleotides at the RNA - DNA junctions in the lysate system are not specific, in contrast to results in permeabilized Escherichia coli."}
{"id": "PMID:1100119", "title": "Wall mannan of Saccharomyces cerevisiae. Metabolic stability and release into growth medium.", "content": "Selective labelling of cell wall mannan with radioactive precursors in growing Saccharomyces cerevisiae showed that this polysaccharide is metabolically stable during exponential growth. Mannan once inserted into the wall is not subject to turnover or release into the growth medium. However, about 10% of the amount of mannan incorporated into the cell wall fraction can be recovered in the non-dialyzable material isolated from the growth medium. Therefore, the mannan escaping from the cell must be either a mannan de novo synthesized, not trapped in the growing wall structure, or a mannan with a non-structural role. Radioactivity was also retained in the wall fraction of cells pre-labelled with [14C] glucose which pointed to metabolic stability of all cell wall polysaccharides in growing S. cerevisiae.", "contents": "Wall mannan of Saccharomyces cerevisiae. Metabolic stability and release into growth medium. Selective labelling of cell wall mannan with radioactive precursors in growing Saccharomyces cerevisiae showed that this polysaccharide is metabolically stable during exponential growth. Mannan once inserted into the wall is not subject to turnover or release into the growth medium. However, about 10% of the amount of mannan incorporated into the cell wall fraction can be recovered in the non-dialyzable material isolated from the growth medium. Therefore, the mannan escaping from the cell must be either a mannan de novo synthesized, not trapped in the growing wall structure, or a mannan with a non-structural role. Radioactivity was also retained in the wall fraction of cells pre-labelled with [14C] glucose which pointed to metabolic stability of all cell wall polysaccharides in growing S. cerevisiae."}
{"id": "PMID:1100120", "title": "Levels and turnover of the proteinase B inhibitors in yeast.", "content": "The ratio of the proteinase B inhibitors IB1 and IB2 from baker's yeast was shown to depend on the yeast strain by specific immunoprecipitation from boiled yeast extract and subsequent electrophoresis of the heat-dissociated precipitates on polyacrylamide gels. Bothe IB1 and IB2 were found, IB2 being by far predominant. Saccharomyces carlsbergensis NCYC 74 contained IB1, whereas in Saccharomyces cerevisiae X 2180 only IB2 was present. When cells of the latter strain were labelled with [14C] leucine from the beginning of growth and pulsed with [3H] leucine during the stationary phase, no short-lived IB1 could be detected. However, the peak of IB2 resolved on the gel showed an increased 3H/14C ratio in comparison to the majority of the other cellular proteins. The increased 3H/14C ratio was found to be the result of catabolite repression of inhibitor synthesis during exponential growth: cells growing on glucose as carbon source contain high inhibitor levels only during the stationary phase of growth, whereas during growth on acetate high amounts of inhibitor are present even in exponentially growing cells. During the stationary phase of growth the inhibitor is degraded with the same half-life as the total cellular proteins (about 50 h).", "contents": "Levels and turnover of the proteinase B inhibitors in yeast. The ratio of the proteinase B inhibitors IB1 and IB2 from baker's yeast was shown to depend on the yeast strain by specific immunoprecipitation from boiled yeast extract and subsequent electrophoresis of the heat-dissociated precipitates on polyacrylamide gels. Bothe IB1 and IB2 were found, IB2 being by far predominant. Saccharomyces carlsbergensis NCYC 74 contained IB1, whereas in Saccharomyces cerevisiae X 2180 only IB2 was present. When cells of the latter strain were labelled with [14C] leucine from the beginning of growth and pulsed with [3H] leucine during the stationary phase, no short-lived IB1 could be detected. However, the peak of IB2 resolved on the gel showed an increased 3H/14C ratio in comparison to the majority of the other cellular proteins. The increased 3H/14C ratio was found to be the result of catabolite repression of inhibitor synthesis during exponential growth: cells growing on glucose as carbon source contain high inhibitor levels only during the stationary phase of growth, whereas during growth on acetate high amounts of inhibitor are present even in exponentially growing cells. During the stationary phase of growth the inhibitor is degraded with the same half-life as the total cellular proteins (about 50 h)."}
{"id": "PMID:1100121", "title": "The use of computed activity coefficients in Raoult's reference system, for n-alcohol/water mixtures, and their application to the study of proteins.", "content": "Activity coefficients of alcohols in Raoult's reference system have been calculated. Using data from papers dealing with transition temperatures in the presence of n-alcohols, lipase deactivation and competitive inhibition by n-alcohols of pepsin and alpha-chymotrypsin, it is shown that the conversion of concentrations into activities (using Pierotti's equation) profoundly affects the original data. Within experimental error, if inhibition constants by alcohols keep the same value, lupase deactivation becomes independent of the length of the alcohol chain, and except for butanol, transition temperature is no longer dependent on the number of methylene groups. Interpretations which take into account the thermodynamic reference system and final environment are related to the fact that alcohol molecules bind to some inner parts of the proteins.", "contents": "The use of computed activity coefficients in Raoult's reference system, for n-alcohol/water mixtures, and their application to the study of proteins. Activity coefficients of alcohols in Raoult's reference system have been calculated. Using data from papers dealing with transition temperatures in the presence of n-alcohols, lipase deactivation and competitive inhibition by n-alcohols of pepsin and alpha-chymotrypsin, it is shown that the conversion of concentrations into activities (using Pierotti's equation) profoundly affects the original data. Within experimental error, if inhibition constants by alcohols keep the same value, lupase deactivation becomes independent of the length of the alcohol chain, and except for butanol, transition temperature is no longer dependent on the number of methylene groups. Interpretations which take into account the thermodynamic reference system and final environment are related to the fact that alcohol molecules bind to some inner parts of the proteins."}
{"id": "PMID:1100122", "title": "A novel electrophoretic fractionation of Escherichia coli envelopes.", "content": "Particulate fractions of Escherichia coli have been submitted to electrophoretic fractionation in a buffer stabilized by sucrose gradient. Inner membrane and outer membrane were readily resolved. A combination of electrophoresis, fractional centrifugation and gel filtration can remove remaining contamination by ribosomes and cytoplasm. The presence of particles containing no phospholipids was detected after differential centrifugation. The nature of this fraction is unknown. The inner membrane exhibited heterogeneity on electrophoresis.", "contents": "A novel electrophoretic fractionation of Escherichia coli envelopes. Particulate fractions of Escherichia coli have been submitted to electrophoretic fractionation in a buffer stabilized by sucrose gradient. Inner membrane and outer membrane were readily resolved. A combination of electrophoresis, fractional centrifugation and gel filtration can remove remaining contamination by ribosomes and cytoplasm. The presence of particles containing no phospholipids was detected after differential centrifugation. The nature of this fraction is unknown. The inner membrane exhibited heterogeneity on electrophoresis."}
{"id": "PMID:1100123", "title": "Inducible gluconate permease in a gluconate kinase-deficient mutant of Escherichia coli.", "content": "Gluconate-resistant mutants were isolated from Escherichia coli strain DF 1070 deficient in phosphogluconate dehydrogenase (EC 1.1.1.44) and in phosphogluconate dehydrogenase (EC 4.2.1.12) which is inhibited by gluconate. Among the resistant mutants, AR 13 has been identified as a gluconate kinase (EC 2.7.1.12)-deficient strain. This mutant exhibits an inducible gluconate transport system capable of concentrating gluconate in the cytoplasm against a concentration gradient. The accumulated gluconate is subject to permanent turnover, and is not chemically modified. The kinetics of induction and deinduction indicate a single inducible component, rate limiting for the transport function, and the distribution of transport capacity among non-induced progeny of induced parents indicates that the inducible protein is membrane bound.", "contents": "Inducible gluconate permease in a gluconate kinase-deficient mutant of Escherichia coli. Gluconate-resistant mutants were isolated from Escherichia coli strain DF 1070 deficient in phosphogluconate dehydrogenase (EC 1.1.1.44) and in phosphogluconate dehydrogenase (EC 4.2.1.12) which is inhibited by gluconate. Among the resistant mutants, AR 13 has been identified as a gluconate kinase (EC 2.7.1.12)-deficient strain. This mutant exhibits an inducible gluconate transport system capable of concentrating gluconate in the cytoplasm against a concentration gradient. The accumulated gluconate is subject to permanent turnover, and is not chemically modified. The kinetics of induction and deinduction indicate a single inducible component, rate limiting for the transport function, and the distribution of transport capacity among non-induced progeny of induced parents indicates that the inducible protein is membrane bound."}
{"id": "PMID:1100126", "title": "Heterogeneity of schizophrenia.", "content": "One of the difficulties in defining schizophrenia is the possibility of its heterogeneity. Schizophrenia may be divided into organic and idiopathic on the basis of precipitating factors; the difference between the two is supported by evidence from family studies and differences in symptomatology. A further division of idiopathic schizophrenia may be made into typical and atypical schizophrenia based on differences in clinical features, family history, mode of inheritance, and prognosis. There is suggestive evidence that within typical schizophrenia, paranoid and nonparanoid subtypes may be idstinguished due to clinical and family differences. Within nonparanoid schizophrenia, simple, catatonic, and hebephrenic schizophrenia may also prove to be distinct entities. The available evidence for differentiating the subtypes of typical schizophrenia is inconclusive to date. However, several recent developments in the methodology of family research may lead to more convincing findings for differentiating subtypes within typical schizophrenia.", "contents": "Heterogeneity of schizophrenia. One of the difficulties in defining schizophrenia is the possibility of its heterogeneity. Schizophrenia may be divided into organic and idiopathic on the basis of precipitating factors; the difference between the two is supported by evidence from family studies and differences in symptomatology. A further division of idiopathic schizophrenia may be made into typical and atypical schizophrenia based on differences in clinical features, family history, mode of inheritance, and prognosis. There is suggestive evidence that within typical schizophrenia, paranoid and nonparanoid subtypes may be idstinguished due to clinical and family differences. Within nonparanoid schizophrenia, simple, catatonic, and hebephrenic schizophrenia may also prove to be distinct entities. The available evidence for differentiating the subtypes of typical schizophrenia is inconclusive to date. However, several recent developments in the methodology of family research may lead to more convincing findings for differentiating subtypes within typical schizophrenia."}
{"id": "PMID:1100130", "title": "Sequential treatment assignment with balancing for prognostic factors in the controlled clinical trial.", "content": "In controlled clinical trials there are usually several prognostic factors known or thought to influence the patient's ability to respond to treatment. Therefore, the method of sequential treatment assignment needs to be designed so that treatment balance is simultaneously achieved across all such patients factor. Traditional methods of restricted randomization such as \"permuted blocks within strata\" prove inadequate once the number of strata, or combinations of factor levels, approaches the sample size. A new general procedure for treatment assignment is described which concentrates on minimizing imbalance in the distributions of treatment numbers within the levels of each individual prognostic factor. The improved treatment balance obtained by this approach is explored using simulation for a simple model of a clinical trial. Further discussion centers on the selection, predictability and practicability of such a procedure.", "contents": "Sequential treatment assignment with balancing for prognostic factors in the controlled clinical trial. In controlled clinical trials there are usually several prognostic factors known or thought to influence the patient's ability to respond to treatment. Therefore, the method of sequential treatment assignment needs to be designed so that treatment balance is simultaneously achieved across all such patients factor. Traditional methods of restricted randomization such as \"permuted blocks within strata\" prove inadequate once the number of strata, or combinations of factor levels, approaches the sample size. A new general procedure for treatment assignment is described which concentrates on minimizing imbalance in the distributions of treatment numbers within the levels of each individual prognostic factor. The improved treatment balance obtained by this approach is explored using simulation for a simple model of a clinical trial. Further discussion centers on the selection, predictability and practicability of such a procedure."}
{"id": "PMID:1100131", "title": "Cost evaluation of a two-stage classification procedure.", "content": "A two-stage classification rule is suggested for classifying an individual into one of two multivariate normal populations. An individual may be classified using measurements on just the first subset; otherwise the second subset is measured and he is classified using the combined set. On the basis of misclassification costs and costs of collecting observations, the two-stage rule is compared with the usual rules based on the first subset and on the combined set. Comparisons are made for the case of known population parameters and also for the case of unknown population parameters which are estimated from samples. It is assumed throughout that a priori probabilities, cost of misclassification, and cost of making observation are known.", "contents": "Cost evaluation of a two-stage classification procedure. A two-stage classification rule is suggested for classifying an individual into one of two multivariate normal populations. An individual may be classified using measurements on just the first subset; otherwise the second subset is measured and he is classified using the combined set. On the basis of misclassification costs and costs of collecting observations, the two-stage rule is compared with the usual rules based on the first subset and on the combined set. Comparisons are made for the case of known population parameters and also for the case of unknown population parameters which are estimated from samples. It is assumed throughout that a priori probabilities, cost of misclassification, and cost of making observation are known."}
{"id": "PMID:1100132", "title": "A generalized modified-chi2 analysis of categorical bacteria survival data from a complex dilution experiment.", "content": "A well-known method of estimation for the density of bacteria in a sample solution is the most probable number (MPN) procedure. This paper considers the change in density through time of bacteria populations which are undergoing extinction. The MPN with its estimated variance for a fixed time point is a basic module of these investigations and is treated as a general implicit function of the cell proportions in linear categorical data analysis. These quantities are then used to fit exponential decay models over time by weighted least squares. When such models are supported by the data, comparisons of decay rates between populations under possibly different experimental conditions can be undertaken. This methodology is illustrated with an example pertaining to the survival experience of Leptospira autumnalis.", "contents": "A generalized modified-chi2 analysis of categorical bacteria survival data from a complex dilution experiment. A well-known method of estimation for the density of bacteria in a sample solution is the most probable number (MPN) procedure. This paper considers the change in density through time of bacteria populations which are undergoing extinction. The MPN with its estimated variance for a fixed time point is a basic module of these investigations and is treated as a general implicit function of the cell proportions in linear categorical data analysis. These quantities are then used to fit exponential decay models over time by weighted least squares. When such models are supported by the data, comparisons of decay rates between populations under possibly different experimental conditions can be undertaken. This methodology is illustrated with an example pertaining to the survival experience of Leptospira autumnalis."}
{"id": "PMID:1100133", "title": "Analysis of data from multiclinic experiments.", "content": "The analysis of data from multiclinic experiments is put in the framework of a mixed model in which the treatment effects are assumed to be fixed, and clinic and clinic by treatment effects are assumed to be random. The analysis in the unbalanced case is developed under the usual mixed model assumptions. The maximum likelihood estimates and likelihood ratio tests of the parameters are derived following the approach of Hemmerle and Hartley (1973). The T2 test is shown to be asymptotically equivalent to the analysis of variance F test in the balanced case. The distribution of the test statistic in the case of proportional cell frequencies is also discussed. Some investigation is made of the distribution of the test statistic by applying the method of Box (1954).", "contents": "Analysis of data from multiclinic experiments. The analysis of data from multiclinic experiments is put in the framework of a mixed model in which the treatment effects are assumed to be fixed, and clinic and clinic by treatment effects are assumed to be random. The analysis in the unbalanced case is developed under the usual mixed model assumptions. The maximum likelihood estimates and likelihood ratio tests of the parameters are derived following the approach of Hemmerle and Hartley (1973). The T2 test is shown to be asymptotically equivalent to the analysis of variance F test in the balanced case. The distribution of the test statistic in the case of proportional cell frequencies is also discussed. Some investigation is made of the distribution of the test statistic by applying the method of Box (1954)."}
{"id": "PMID:1100134", "title": "Statistics and medical experimentation.", "content": "Starting from a review of four major experimental areas in medicine (Polio, Coronary Surgery, Diabetes, Breast Cancer), some of the special statistical problems arising in the design and analysis of clinical experiments are reviewed, and the limitations of current formulations are emphasized. Particular attention is given to the ethical dilemma and an alternative formulation of the inference/decision problem is proposed.", "contents": "Statistics and medical experimentation. Starting from a review of four major experimental areas in medicine (Polio, Coronary Surgery, Diabetes, Breast Cancer), some of the special statistical problems arising in the design and analysis of clinical experiments are reviewed, and the limitations of current formulations are emphasized. Particular attention is given to the ethical dilemma and an alternative formulation of the inference/decision problem is proposed."}
{"id": "PMID:1100135", "title": "Randomization and the analysis of variance.", "content": "This paper attempts to strengthen faith in randomization, first by defining it in general and second by showing how it is the fundamental means, in many experiments, of generating the probability space. It is defined by the natural structure of the experimental units (E.U.'s) not by a particular experimental design imposed on that structure. Three examples are given, with the same design but different E.U. structures and randomizations, to show that models developed from experimental designs are superficial, but that their randomization is fundamental.", "contents": "Randomization and the analysis of variance. This paper attempts to strengthen faith in randomization, first by defining it in general and second by showing how it is the fundamental means, in many experiments, of generating the probability space. It is defined by the natural structure of the experimental units (E.U.'s) not by a particular experimental design imposed on that structure. Three examples are given, with the same design but different E.U. structures and randomizations, to show that models developed from experimental designs are superficial, but that their randomization is fundamental."}
{"id": "PMID:1100136", "title": "Efficiency of case-control studies with multiple controls per case: continuous or dichotomous data.", "content": "The statistical efficiency of a study design which matches each case with k controls is compared with that of the standard matched-pairs setup. For the situation in which the distribution of measurements on the factor under investigation is continuous, the comparison criterion used is the reciprocal of the variance ratio of the corresponding case-control difference estimators; for dichotomous observations, the Pitman efficiency of Miettinen's test [1969] or of Pike and Morrow's test [1970] relative to McNemar's test is used. When the variances for continuously distributed case and control measurements are equal to the same result, 2k/(k + 1), is obtained for both situations and, more generally, the efficiency of k1 controls relative to k2 controls is given by k1(k2 + 1)/k2(k1 + 1). The Pitman efficiency is compared with the \"practical efficiency\" for large-sample dichotomous situations.", "contents": "Efficiency of case-control studies with multiple controls per case: continuous or dichotomous data. The statistical efficiency of a study design which matches each case with k controls is compared with that of the standard matched-pairs setup. For the situation in which the distribution of measurements on the factor under investigation is continuous, the comparison criterion used is the reciprocal of the variance ratio of the corresponding case-control difference estimators; for dichotomous observations, the Pitman efficiency of Miettinen's test [1969] or of Pike and Morrow's test [1970] relative to McNemar's test is used. When the variances for continuously distributed case and control measurements are equal to the same result, 2k/(k + 1), is obtained for both situations and, more generally, the efficiency of k1 controls relative to k2 controls is given by k1(k2 + 1)/k2(k1 + 1). The Pitman efficiency is compared with the \"practical efficiency\" for large-sample dichotomous situations."}
{"id": "PMID:1100137", "title": "The relationship between separation time and genetic distance based on angular transformations of gene frequencies.", "content": "Expressions are constructed for the theoretical expectation and the variance of the statistic chord2 used in connection with the genetic distances introduced by Cavalli-Sforza and Edwards. The gene frequency probability distribution is taken from Kimura's solution of the diffusion equation for pure random drift. The expression for the expectation is used for discussing whether distances are independent of original gene frequencies, and whether they are proportional to separation times. A modified method for estimating such separation times is proposed, and an example is given with cattle breeds. The accuracy of procedures based on genetic distances is evaluated using the variance of chord2.", "contents": "The relationship between separation time and genetic distance based on angular transformations of gene frequencies. Expressions are constructed for the theoretical expectation and the variance of the statistic chord2 used in connection with the genetic distances introduced by Cavalli-Sforza and Edwards. The gene frequency probability distribution is taken from Kimura's solution of the diffusion equation for pure random drift. The expression for the expectation is used for discussing whether distances are independent of original gene frequencies, and whether they are proportional to separation times. A modified method for estimating such separation times is proposed, and an example is given with cattle breeds. The accuracy of procedures based on genetic distances is evaluated using the variance of chord2."}
{"id": "PMID:1100141", "title": "F-cells in the adult: normal values and levels in individuals with hereditary and acquired elevations of Hb F.", "content": "Specific antibodies to human fetal hemoglobin were prepared and, after conjugation with a fluorescent dye, were used to determine the distribution of Hb F-containing cells in blood smears from normal adults and individuals with hereditary and acquired conditions associated with abnormal levels of Hb F. The mean proportion of F-cells in normal persons was 2.7% +/- 1.4%, with a range of 0.5%-7.0%. Proportions of F-cells were increased in persons with several acquired and inherited disorders that are associated with an increased percentage of Hb F in hemolysates. A strong linear correlation between the amount of Hb F and proportion of F-cells was observed. This technique may prove useful in studies of a variety of disorders associated with Hb F elevations and also in investigations of the mechanisms controlling the transition from fetal to adult hemoglobin during the course of human development.", "contents": "F-cells in the adult: normal values and levels in individuals with hereditary and acquired elevations of Hb F. Specific antibodies to human fetal hemoglobin were prepared and, after conjugation with a fluorescent dye, were used to determine the distribution of Hb F-containing cells in blood smears from normal adults and individuals with hereditary and acquired conditions associated with abnormal levels of Hb F. The mean proportion of F-cells in normal persons was 2.7% +/- 1.4%, with a range of 0.5%-7.0%. Proportions of F-cells were increased in persons with several acquired and inherited disorders that are associated with an increased percentage of Hb F in hemolysates. A strong linear correlation between the amount of Hb F and proportion of F-cells was observed. This technique may prove useful in studies of a variety of disorders associated with Hb F elevations and also in investigations of the mechanisms controlling the transition from fetal to adult hemoglobin during the course of human development."}
{"id": "PMID:1100143", "title": "Adrenergic mechanisms in the rabbit ear artery. A review.", "content": "A number of factors which have been implicated in the response of the rabbit ear artery to adrenergic agents and stionstriction in the arterial smooth muscle; (b) the interrelationship between the intramural distribution of sympathetic nerves and muscle, and the influence of uptake and enzymic inactivation on the response to norepinephrine, and (c) the influence of presynaptic adrenergic and cholinergic receptors on the rwsponse to sympathetic nerve stimulation.", "contents": "Adrenergic mechanisms in the rabbit ear artery. A review. A number of factors which have been implicated in the response of the rabbit ear artery to adrenergic agents and stionstriction in the arterial smooth muscle; (b) the interrelationship between the intramural distribution of sympathetic nerves and muscle, and the influence of uptake and enzymic inactivation on the response to norepinephrine, and (c) the influence of presynaptic adrenergic and cholinergic receptors on the rwsponse to sympathetic nerve stimulation."}
{"id": "PMID:1100145", "title": "The NBT test: Erratic behaviour in acute leukaemia.", "content": "Non-stimulated and stimulated nitroblue tetrazolium (NBT) dye test was studied in sixteen adult patients with acute leukaemia in sequence, viz. at the time of diagnosis, during and after treatment. It was found that the capacity of the neutrophilic granulocytes to reduce NBT (non-stimulated) was below, within or above the range for the normal group. Two of the patients had a constant negative non-stimulated NBT test. The stimulated NBT test was below the normal range. Generally, there was no correlation between the percentage of NBT positive cells and fever of bacterial origin. All sixteen patients but four were found to be within the normal range concerning phagocytic ability.", "contents": "The NBT test: Erratic behaviour in acute leukaemia. Non-stimulated and stimulated nitroblue tetrazolium (NBT) dye test was studied in sixteen adult patients with acute leukaemia in sequence, viz. at the time of diagnosis, during and after treatment. It was found that the capacity of the neutrophilic granulocytes to reduce NBT (non-stimulated) was below, within or above the range for the normal group. Two of the patients had a constant negative non-stimulated NBT test. The stimulated NBT test was below the normal range. Generally, there was no correlation between the percentage of NBT positive cells and fever of bacterial origin. All sixteen patients but four were found to be within the normal range concerning phagocytic ability."}
{"id": "PMID:1100146", "title": "Effect on platelet counts and fever of platelet transfusion in leukemia.", "content": "The efficacy of platelet transfusions in pretransfused HL-1 sensitized patients has been doubtful, particularly where repeated platelet transfusions were required. The efficacy of one hundred and nine ABO-compatible, cross match negative platelet transfusions in 26 adult patients with acute leukemia was therefore studied. Although most of the patientswere old and pretransfused and although no HL-A typing could be made, a statistically significant average increase of about 11 000 platelets/mul was seen not only after the first but also after subsequent (up to 8) platelet transfusions. Moreover, significant reduction in temperature followed platelet transfusions. However, platelet recoveries were worse in severely infected patients.", "contents": "Effect on platelet counts and fever of platelet transfusion in leukemia. The efficacy of platelet transfusions in pretransfused HL-1 sensitized patients has been doubtful, particularly where repeated platelet transfusions were required. The efficacy of one hundred and nine ABO-compatible, cross match negative platelet transfusions in 26 adult patients with acute leukemia was therefore studied. Although most of the patientswere old and pretransfused and although no HL-A typing could be made, a statistically significant average increase of about 11 000 platelets/mul was seen not only after the first but also after subsequent (up to 8) platelet transfusions. Moreover, significant reduction in temperature followed platelet transfusions. However, platelet recoveries were worse in severely infected patients."}
{"id": "PMID:1100156", "title": "Depolarizing effects of the ionophores X-537A and A23187 and their relevance to secretion.", "content": "The ionophore X-537A depolarized frog skeletal muscle fibres about 10 mV in 10 min and a further 30 mV or so over the next 50 minutes. With A23187, depolarization was relatively feeble and delayed in onset but was hastened when calcium was removed from the bathing medium. The results support conjecture that some functional responses to these ionophores are attributable, in part, to depolarization.", "contents": "Depolarizing effects of the ionophores X-537A and A23187 and their relevance to secretion. The ionophore X-537A depolarized frog skeletal muscle fibres about 10 mV in 10 min and a further 30 mV or so over the next 50 minutes. With A23187, depolarization was relatively feeble and delayed in onset but was hastened when calcium was removed from the bathing medium. The results support conjecture that some functional responses to these ionophores are attributable, in part, to depolarization."}
{"id": "PMID:1100157", "title": "Studies on the hypotensive action of alpha-methyldopamine.", "content": "1. Intraventricular alpha-methyldopamine (50-200 mug) produced a dose-related fall in blood pressure in conscious spontaneously hypertensive rats. Pretreatment with intraventricular 6-hydroxydopamine prevented this hypotensive effect of alpha-methyldopamine. 2. The hypotensive effect of alpha-methyldopamine was prevented by intraventricular injection of phentolamine or desmethylimipramine, but not by intraperitoneal injection of haloperidol. 3. Pretreatment with U-14,624, a selective central dopamine-beta-hydroxylase inhibitor, prevented the hypotensive effect of alpha-methyldopamine. 4. Alpha-methyldopamine was considerably less potent than noradrenaline as a pressor agent in the pithed rat, but noradrenaline and alpha-methylnoradrenaline were found to be equipotent. 5. Alpha-methyldopamine (1-5 mg i.c.v.) reduced pressor responses elicited by electrical stimulation of the midbrain reticular formation in cats anaesthetized with chloralose. 6. It is concluded that the hypotensive action of alpha-methyldopamine in conscious animals involves intact central alpha-adrenergic neurones and a central adrenergic uptake mechanism for the formation of alpha-methylnoradrenaline.", "contents": "Studies on the hypotensive action of alpha-methyldopamine. 1. Intraventricular alpha-methyldopamine (50-200 mug) produced a dose-related fall in blood pressure in conscious spontaneously hypertensive rats. Pretreatment with intraventricular 6-hydroxydopamine prevented this hypotensive effect of alpha-methyldopamine. 2. The hypotensive effect of alpha-methyldopamine was prevented by intraventricular injection of phentolamine or desmethylimipramine, but not by intraperitoneal injection of haloperidol. 3. Pretreatment with U-14,624, a selective central dopamine-beta-hydroxylase inhibitor, prevented the hypotensive effect of alpha-methyldopamine. 4. Alpha-methyldopamine was considerably less potent than noradrenaline as a pressor agent in the pithed rat, but noradrenaline and alpha-methylnoradrenaline were found to be equipotent. 5. Alpha-methyldopamine (1-5 mg i.c.v.) reduced pressor responses elicited by electrical stimulation of the midbrain reticular formation in cats anaesthetized with chloralose. 6. It is concluded that the hypotensive action of alpha-methyldopamine in conscious animals involves intact central alpha-adrenergic neurones and a central adrenergic uptake mechanism for the formation of alpha-methylnoradrenaline."}
{"id": "PMID:1100159", "title": "Bile salts and gallstone disease.", "content": "Bile salts play an important role in maintaining cholesterol in aqueous solution in bile. There is evidence that in some patients at least gallstones arise as a result of bile salt deficiency. The evidence to date suggests that although oral bile salts may be of use in treating some gallstone patients they are unlikely to replace cholecystectomy in the foreseeable future. The instillation of bile salts via an indwelling T-tube may well be of great use in the management of stones retained in the common duct after choledocholithotomy. There is no doubt that in this branch of medicine as in all others prevention is better than cure and our aim should be to perfect our technique so that we do not leave stones behind. However, should the situation occur we may soon have safe effective physiological solvents to dissolve our mistakes.", "contents": "Bile salts and gallstone disease. Bile salts play an important role in maintaining cholesterol in aqueous solution in bile. There is evidence that in some patients at least gallstones arise as a result of bile salt deficiency. The evidence to date suggests that although oral bile salts may be of use in treating some gallstone patients they are unlikely to replace cholecystectomy in the foreseeable future. The instillation of bile salts via an indwelling T-tube may well be of great use in the management of stones retained in the common duct after choledocholithotomy. There is no doubt that in this branch of medicine as in all others prevention is better than cure and our aim should be to perfect our technique so that we do not leave stones behind. However, should the situation occur we may soon have safe effective physiological solvents to dissolve our mistakes."}
{"id": "PMID:1100160", "title": "Orthotopic liver transplantation: an experimental study on the prevention of infections with Gram-negative organisms.", "content": "Infection with Gram-negative bacteria is frequently the cause of death in human liver transplantation. In experimental liver transplantation in mongrel dogs we detected Gram-negative septicaemia in 73% of our first 15 cases; maximal survival was 26 days. In a second series of 27 orthotopic liver transplantations in tissue-typed littermate dogs and littermate pigs the standard surgical technique and aftercare were changed with regard to four factors: an end-to-end common bile duct anastomosis was made instead of a gallbladder to duodenum anastomosis; the continuous postoperative bacteriostatic antibiotic therapy was changed to a single 2-day course of bactericidal antibiotics; preoperative selective bowel decontamination combined with postoperative protective isolation was introduced; the dosage of azathioprine used for immunosuppression was reduced..", "contents": "Orthotopic liver transplantation: an experimental study on the prevention of infections with Gram-negative organisms. Infection with Gram-negative bacteria is frequently the cause of death in human liver transplantation. In experimental liver transplantation in mongrel dogs we detected Gram-negative septicaemia in 73% of our first 15 cases; maximal survival was 26 days. In a second series of 27 orthotopic liver transplantations in tissue-typed littermate dogs and littermate pigs the standard surgical technique and aftercare were changed with regard to four factors: an end-to-end common bile duct anastomosis was made instead of a gallbladder to duodenum anastomosis; the continuous postoperative bacteriostatic antibiotic therapy was changed to a single 2-day course of bactericidal antibiotics; preoperative selective bowel decontamination combined with postoperative protective isolation was introduced; the dosage of azathioprine used for immunosuppression was reduced.."}
{"id": "PMID:1100162", "title": "Gastric acid secretion following renal transplantation.", "content": "Gastric acid secretion studies were performed in 10 patients on regular dialysis and again following transplantation. While on dialysis, the acid output was high, with mean peak values after pentagastrin of 37-2 mEq/h in males and 26-3 mEq/h in females. These compared with values in normal subjects of 21-6 mEq/h in males and 12-3 mEq/h in females. Following transplantation continuous steroid therapy was given. Repear gastric acid secretion studies showed no significant change, with values of 37-1 mEq/h in males and 24-6 mEq/h in females. The high incidence of gastro-intestinal haemorrhage and perforation following transplantation is probably due mainly to steroid therapy, but the ulcerogenic effect of steroids would not seem to be mediated by increased gastric acid secretion.", "contents": "Gastric acid secretion following renal transplantation. Gastric acid secretion studies were performed in 10 patients on regular dialysis and again following transplantation. While on dialysis, the acid output was high, with mean peak values after pentagastrin of 37-2 mEq/h in males and 26-3 mEq/h in females. These compared with values in normal subjects of 21-6 mEq/h in males and 12-3 mEq/h in females. Following transplantation continuous steroid therapy was given. Repear gastric acid secretion studies showed no significant change, with values of 37-1 mEq/h in males and 24-6 mEq/h in females. The high incidence of gastro-intestinal haemorrhage and perforation following transplantation is probably due mainly to steroid therapy, but the ulcerogenic effect of steroids would not seem to be mediated by increased gastric acid secretion."}
{"id": "PMID:1100179", "title": "Analysis of treatment in childhood leukaemia. I. Predisposition to methotrexate-induced neutropenia after craniospinal irradiation. Report to the Medical Research Council of the Working Party on Leukaemia in Childhood.", "content": "The degree of drug-induced neutropenia resulting from a controlled trial (UKALL I) of treatment in acute lymphoblastic leukaemia was analysed. The main agent associated with severe neutropenia was methotrexate, and methotrexate-induced neutropenia was significantly greater in patients who had received craniospinal irradiation. The synergistic toxic effect of irradiation followed by methotrexate treatment seems to have contributed to three of the five deaths which occurred in complete remission in this trial; all deaths in remission occurred in patients who had received central nervous system prophylaxis. Analysis of patients who subsequently relapsed compared with those still in remission after 18 months of treatment indicated that the former, on average, had slightly lower neutrophil counts. This suggests that the children who relapsed did not receive any less aggressive treatment than those who remained in remission.", "contents": "Analysis of treatment in childhood leukaemia. I. Predisposition to methotrexate-induced neutropenia after craniospinal irradiation. Report to the Medical Research Council of the Working Party on Leukaemia in Childhood. The degree of drug-induced neutropenia resulting from a controlled trial (UKALL I) of treatment in acute lymphoblastic leukaemia was analysed. The main agent associated with severe neutropenia was methotrexate, and methotrexate-induced neutropenia was significantly greater in patients who had received craniospinal irradiation. The synergistic toxic effect of irradiation followed by methotrexate treatment seems to have contributed to three of the five deaths which occurred in complete remission in this trial; all deaths in remission occurred in patients who had received central nervous system prophylaxis. Analysis of patients who subsequently relapsed compared with those still in remission after 18 months of treatment indicated that the former, on average, had slightly lower neutrophil counts. This suggests that the children who relapsed did not receive any less aggressive treatment than those who remained in remission."}
{"id": "PMID:1100180", "title": "Serum digoxin in patients with thyroid disease.", "content": "Serum digoxin concentrations were measured by radioimmunoassay in 17 hyperthyroid and 16 hypothyroid patients after a seven-day course of oral digoxin. The significantly higher levels of serum digoxin in patients with hypothyroidism and lower levels in those with hyperthyroidism were closely related to the measured changes of glomerular filtration rate and digoxin serum half time in these two groups. Differences in serum digoxin concentration contribute to the altered sensitivity to digoxin shown by patients with thyroid disease.", "contents": "Serum digoxin in patients with thyroid disease. Serum digoxin concentrations were measured by radioimmunoassay in 17 hyperthyroid and 16 hypothyroid patients after a seven-day course of oral digoxin. The significantly higher levels of serum digoxin in patients with hypothyroidism and lower levels in those with hyperthyroidism were closely related to the measured changes of glomerular filtration rate and digoxin serum half time in these two groups. Differences in serum digoxin concentration contribute to the altered sensitivity to digoxin shown by patients with thyroid disease."}
{"id": "PMID:1100181", "title": "Use of bacteriological investigations by general practitioners.", "content": "The numbers of vaginal swabs, faecal specimens, throat swabs, and urine specimens submitted by 104 city general practitioners to the bacteriology laboratory at Aberdeen differed widely. The doctors who made most use of the laboratory service rarely did so equally for all four investigations but usually for only one or two. Similarly, those who used the service least often made frequent use of one particular investigation. Further studies are needed to identify and evaluate the reasons for the apparently substantial disagreement among general practitioners concerning the value of these diffferent bacteriological investigations in general practice.", "contents": "Use of bacteriological investigations by general practitioners. The numbers of vaginal swabs, faecal specimens, throat swabs, and urine specimens submitted by 104 city general practitioners to the bacteriology laboratory at Aberdeen differed widely. The doctors who made most use of the laboratory service rarely did so equally for all four investigations but usually for only one or two. Similarly, those who used the service least often made frequent use of one particular investigation. Further studies are needed to identify and evaluate the reasons for the apparently substantial disagreement among general practitioners concerning the value of these diffferent bacteriological investigations in general practice."}
{"id": "PMID:1100186", "title": "Agonal phase, ischaemic times, and renal vascular abnormalities and outcome of cadaver kidney transplants.", "content": "A retrospective study of 250 cadaver kidney transplants was carried out to determine the effects of the agonal period, the warm and cold ischaemic times, and the use of kidneys with vascular anomalies on the primary success and failure and the subsequent level of function of the transplants. Kidneys with vascular anomalies or from non-ventilated donors had a primary failure rate of over 30%, whereas those with normal vasculature or from ventilated donors had a rate of 17%. An initial warm ischaemic time of more than 60 minutes was associated with a primary failure rate of 57% and a cold ischaemic time of over 550 minutes with a primary failure rate of 47%. The interrelationship between the warm and cold ischaemic times in the primary success or failure of the transplants was examined and criteria defined for selecting potentially viable cadaver kidneys for transplantation, as follows: (1) The donor should be (a) ventilated, (b) aged 6-50 years, and (c) have normal ante-mortem renal function and have secreted more than 1-5 1 of urine in the 24 hours before death (or an equivalent volume if the urinary output was recorded for less than 24 hours before death); (2) the kidney should have normal renal vasculature enabling single arterial and venous anastomoses to be performed; (3) kidneys with I.W.I.T.s of longer than 60 minutes should not be used; (4) for kidneys with I.W.I.T.s of less than 20 minutes the C.I.T. is not critical but should not exceed 12 hours; (5) for kidneys with I.W.I.T.s of 20-60 minutes the C.I.T. should not exceed 450 minutes.", "contents": "Agonal phase, ischaemic times, and renal vascular abnormalities and outcome of cadaver kidney transplants. A retrospective study of 250 cadaver kidney transplants was carried out to determine the effects of the agonal period, the warm and cold ischaemic times, and the use of kidneys with vascular anomalies on the primary success and failure and the subsequent level of function of the transplants. Kidneys with vascular anomalies or from non-ventilated donors had a primary failure rate of over 30%, whereas those with normal vasculature or from ventilated donors had a rate of 17%. An initial warm ischaemic time of more than 60 minutes was associated with a primary failure rate of 57% and a cold ischaemic time of over 550 minutes with a primary failure rate of 47%. The interrelationship between the warm and cold ischaemic times in the primary success or failure of the transplants was examined and criteria defined for selecting potentially viable cadaver kidneys for transplantation, as follows: (1) The donor should be (a) ventilated, (b) aged 6-50 years, and (c) have normal ante-mortem renal function and have secreted more than 1-5 1 of urine in the 24 hours before death (or an equivalent volume if the urinary output was recorded for less than 24 hours before death); (2) the kidney should have normal renal vasculature enabling single arterial and venous anastomoses to be performed; (3) kidneys with I.W.I.T.s of longer than 60 minutes should not be used; (4) for kidneys with I.W.I.T.s of less than 20 minutes the C.I.T. is not critical but should not exceed 12 hours; (5) for kidneys with I.W.I.T.s of 20-60 minutes the C.I.T. should not exceed 450 minutes."}
{"id": "PMID:1100187", "title": "Hyperparathyroidism after renal transplantation.", "content": "Renal transplantation reduced circulating parathyroid hormone in recipients of renal allografts. Nevertheless, biochemical and histological evidence of mild hyperparathyroidism persisted up to eight years after renal transplantation despite good graft function.", "contents": "Hyperparathyroidism after renal transplantation. Renal transplantation reduced circulating parathyroid hormone in recipients of renal allografts. Nevertheless, biochemical and histological evidence of mild hyperparathyroidism persisted up to eight years after renal transplantation despite good graft function."}
{"id": "PMID:1100188", "title": "Closure of abdominal wounds by adhesive strips: a clinical trial.", "content": "In a randomized trial of wound closure in 512 abdominal wounds, wounds were closed with either reinforced Steristrip skin closures or interrupted silk sutures. Comparisons were made of wound pain and discomfort, wound infection, discharge, redness, width, and skin reaction. The causes of peeling of the tapes were assessed. The results showed that tapes were significantly more comfortable and that patients preferred them to sutures (P less than 0.01), but wide scars occurred more often. There was no difference in rates of wound infection and no case of allergy to the tapes was seen. Closure of abdominal wounds by these tapes is a satisfactory procedure that could be used more extensively.", "contents": "Closure of abdominal wounds by adhesive strips: a clinical trial. In a randomized trial of wound closure in 512 abdominal wounds, wounds were closed with either reinforced Steristrip skin closures or interrupted silk sutures. Comparisons were made of wound pain and discomfort, wound infection, discharge, redness, width, and skin reaction. The causes of peeling of the tapes were assessed. The results showed that tapes were significantly more comfortable and that patients preferred them to sutures (P less than 0.01), but wide scars occurred more often. There was no difference in rates of wound infection and no case of allergy to the tapes was seen. Closure of abdominal wounds by these tapes is a satisfactory procedure that could be used more extensively."}
{"id": "PMID:1100190", "title": "Reticuloendothelial function in renal allograft recipients.", "content": "The phagocytic capacity of the reticuloendothelial system (R.E.S.) was assessed in patients with chronic renal failure and in renal transplant recipients. R.E.S. phagocytosis was increased in the former group. Soon after transplantation R.E.S. phagocytosis was moderately reduced (through levels were comparable with those of normal controls) but was particularly reduced after high-dose corticosteroid treatment for rejection. In long-term allograft recipients R.E.S. phagocytosis was also depressed though steroid maintenance doses were small.", "contents": "Reticuloendothelial function in renal allograft recipients. The phagocytic capacity of the reticuloendothelial system (R.E.S.) was assessed in patients with chronic renal failure and in renal transplant recipients. R.E.S. phagocytosis was increased in the former group. Soon after transplantation R.E.S. phagocytosis was moderately reduced (through levels were comparable with those of normal controls) but was particularly reduced after high-dose corticosteroid treatment for rejection. In long-term allograft recipients R.E.S. phagocytosis was also depressed though steroid maintenance doses were small."}
{"id": "PMID:1100192", "title": "Survival of infants with unoperated myeloceles.", "content": "Of the infants with spina bifida referred to a neonatal surgical unit over three years and denied early closure of the myelocele, a significant proportion survived long enough for procedures to drain hydrocephalus to be needed. The key decision in the management of this condition is not whether myeloceles should be closed but whether hydrocephalus should be treated.", "contents": "Survival of infants with unoperated myeloceles. Of the infants with spina bifida referred to a neonatal surgical unit over three years and denied early closure of the myelocele, a significant proportion survived long enough for procedures to drain hydrocephalus to be needed. The key decision in the management of this condition is not whether myeloceles should be closed but whether hydrocephalus should be treated."}
{"id": "PMID:1100199", "title": "Ultrastructural localization of luteinizing hormone-releasing hormone in the median eminence of the rat.", "content": "The nature of cells in the hypothalamus that produce hypothalamic hypophysiotropic hormones remains unsettled. To investigate this problem electron microscopic immunocytochemistry was performed on thin sections of the median eminence of proestrous rats using antibodies to synthetic luteinizing hormone-releasing hormone (LHRH) and the peroxidase-antiperoxidase (PAP) technique. PAP complexes indicating the presence of LHRH were found over small, homogeneously dense neurosecretory granules 75-90 nm in diameter that occurred in clusters within neurons. Positive staining was more prevalent in the palisade zone in the anterior median eminence than at more posterior levels. LHRH positive granules were not obsereved in neuronal processes in the neurohemal contact zone, but appeared closer to the pericapillary space in the posterior median eminence than at more anterior levels. Occasional groups of LHRH positive granules were also found in the internal layer and hypendymal zone. No staining was observed in tanycytes or glial elements. These results support the hypothesis that hypothalamic hypophysiotropic hormones are produced by neurons and are stored in granules of homogeneous size and density in nerve processes  located in the median eminence in the proximity of the hypophysial portal plexus.", "contents": "Ultrastructural localization of luteinizing hormone-releasing hormone in the median eminence of the rat. The nature of cells in the hypothalamus that produce hypothalamic hypophysiotropic hormones remains unsettled. To investigate this problem electron microscopic immunocytochemistry was performed on thin sections of the median eminence of proestrous rats using antibodies to synthetic luteinizing hormone-releasing hormone (LHRH) and the peroxidase-antiperoxidase (PAP) technique. PAP complexes indicating the presence of LHRH were found over small, homogeneously dense neurosecretory granules 75-90 nm in diameter that occurred in clusters within neurons. Positive staining was more prevalent in the palisade zone in the anterior median eminence than at more posterior levels. LHRH positive granules were not obsereved in neuronal processes in the neurohemal contact zone, but appeared closer to the pericapillary space in the posterior median eminence than at more anterior levels. Occasional groups of LHRH positive granules were also found in the internal layer and hypendymal zone. No staining was observed in tanycytes or glial elements. These results support the hypothesis that hypothalamic hypophysiotropic hormones are produced by neurons and are stored in granules of homogeneous size and density in nerve processes  located in the median eminence in the proximity of the hypophysial portal plexus."}
{"id": "PMID:1100203", "title": "Relationships of intestinal enzymes and serum antitoxin to the pig response to Escherichia coli enterotoxin.", "content": "The intestinal loop technique was used to evaluate the response of three week old piglets to the heat labile (LT) and the heat stable (ST) enterotoxins produced by Escherichia coli F11(P155). The serum anti-LT activity and the lipase, amylase and trypsin activities in the jejunal lumen of these pigs were determined. Piglets responded independently ti each toxin and no relationship between these responses and serum anti-LT activity or the enzyme activities of the jejunal content could be demonstrated.", "contents": "Relationships of intestinal enzymes and serum antitoxin to the pig response to Escherichia coli enterotoxin. The intestinal loop technique was used to evaluate the response of three week old piglets to the heat labile (LT) and the heat stable (ST) enterotoxins produced by Escherichia coli F11(P155). The serum anti-LT activity and the lipase, amylase and trypsin activities in the jejunal lumen of these pigs were determined. Piglets responded independently ti each toxin and no relationship between these responses and serum anti-LT activity or the enzyme activities of the jejunal content could be demonstrated."}
{"id": "PMID:1100204", "title": "Polyserositis in pigs due to generalized Escherichia coli infection.", "content": "A fibrinous polyserositis syndrome due to generalized Escherichia coli infection in pigs was observed in 13 out of 17 systematically monitored herds. The mortality rate was approximately 0.1% among liveborn pigs. The occurrence was usually sporadic but a minor enzootic was observed in one herd. Most of the affected pigs succumbed during first or second week of life but cases were observed throughout the suckling period. The clinical signs included marked depression, anorexia, rough haircoat, laboured respiration and death in two to five days. Predominant gross pathological lesions were signs of septicaemia and a voluminous, gelatinous and fibrinous exudate in the pleural, the pericardial and the peritoneal cavities. Frequently also a firbinous polyarthritis and a fibrinous pneumonia were present. The majority of the isolated invasive E. coli strains were nonhaemolytic. Serologically 11 different E. coli O groups were encountered. O group most frequently represented was 020. None of the examined E. coli strains belonged to the serogroups which frequently are associated with enteropathogenicity in pigs.", "contents": "Polyserositis in pigs due to generalized Escherichia coli infection. A fibrinous polyserositis syndrome due to generalized Escherichia coli infection in pigs was observed in 13 out of 17 systematically monitored herds. The mortality rate was approximately 0.1% among liveborn pigs. The occurrence was usually sporadic but a minor enzootic was observed in one herd. Most of the affected pigs succumbed during first or second week of life but cases were observed throughout the suckling period. The clinical signs included marked depression, anorexia, rough haircoat, laboured respiration and death in two to five days. Predominant gross pathological lesions were signs of septicaemia and a voluminous, gelatinous and fibrinous exudate in the pleural, the pericardial and the peritoneal cavities. Frequently also a firbinous polyarthritis and a fibrinous pneumonia were present. The majority of the isolated invasive E. coli strains were nonhaemolytic. Serologically 11 different E. coli O groups were encountered. O group most frequently represented was 020. None of the examined E. coli strains belonged to the serogroups which frequently are associated with enteropathogenicity in pigs."}
{"id": "PMID:1100205", "title": "Immunofluorescent cell assay of neonatal calf diarrhea virus.", "content": "A reliable plaque assay procedure has not yet been described for the neonatal calf diarrhea virus. Therefore, a previously developed immunofluorescent cell counting procedure was adapted to assay this virus. Adsorption of the virus to bovine kidney cells plateaued at 60 minutes. The optimal staining time was between 20 and 24 hours postinfection. Infected cells begun releasing from the coverslips if the cultures were incubated longer than 24 hours. This procedure has proven successful with virus grown in cell culture as well as virus present in fecal samples.", "contents": "Immunofluorescent cell assay of neonatal calf diarrhea virus. A reliable plaque assay procedure has not yet been described for the neonatal calf diarrhea virus. Therefore, a previously developed immunofluorescent cell counting procedure was adapted to assay this virus. Adsorption of the virus to bovine kidney cells plateaued at 60 minutes. The optimal staining time was between 20 and 24 hours postinfection. Infected cells begun releasing from the coverslips if the cultures were incubated longer than 24 hours. This procedure has proven successful with virus grown in cell culture as well as virus present in fecal samples."}
{"id": "PMID:1100206", "title": "Metabolism of [2-14C]prostaglandin E1 on passage through the pulmonary circulation.", "content": "A multiple indicator dilution technique was used to study inactivation of [14C]prostaglandin E1 (PGE1) by isolated cat lungs. A bolus containing [2-14C]PGE1, indocyanine green dye and 3HOH was rapidly introduced into the blood entering the pulmonary artery. Sequential samples of the venous effluent were collected and analyzed for 3H, dye, and 14C. The 14C-labelled compounds were separated by thin-layer chromatography, and the quantity of PGE1 and its metabolites contained in each sample was determined (radiochromatogram scanning). Recovery of the dye and 3H was complete within the sampling period. However, only 79% of the injected 14C emerged from the lung within this period, 36% as [14C]PGE1, and 43% as two less polar metabolites. The mean transit times (including connecting tubing) for the unmetabolized [14C]PGE1 and the dye were 9.1 and 8.7 s, respectively. The apparent mean transit times for 14C in the metabolites were considerably longer, 12.4 and 14.3 s. The 3HOH mean transit time was 10.7 s. These data are compatible with an extraluminal site for PGE1 metabolism.", "contents": "Metabolism of [2-14C]prostaglandin E1 on passage through the pulmonary circulation. A multiple indicator dilution technique was used to study inactivation of [14C]prostaglandin E1 (PGE1) by isolated cat lungs. A bolus containing [2-14C]PGE1, indocyanine green dye and 3HOH was rapidly introduced into the blood entering the pulmonary artery. Sequential samples of the venous effluent were collected and analyzed for 3H, dye, and 14C. The 14C-labelled compounds were separated by thin-layer chromatography, and the quantity of PGE1 and its metabolites contained in each sample was determined (radiochromatogram scanning). Recovery of the dye and 3H was complete within the sampling period. However, only 79% of the injected 14C emerged from the lung within this period, 36% as [14C]PGE1, and 43% as two less polar metabolites. The mean transit times (including connecting tubing) for the unmetabolized [14C]PGE1 and the dye were 9.1 and 8.7 s, respectively. The apparent mean transit times for 14C in the metabolites were considerably longer, 12.4 and 14.3 s. The 3HOH mean transit time was 10.7 s. These data are compatible with an extraluminal site for PGE1 metabolism."}
{"id": "PMID:1100207", "title": "Effective selection of Proteus mirabilis clones producing mirabilicin D-52.", "content": "From a defective-lysogenic Proteus mirabilis strain we isolated several clones differing in the pattern of their growth on agar plates. Using electron microscopy we have shown some of the selected clones to be efficient in producing mirabilicin D-52 after UV induction, while other clones produced defective mirabilicin polysheaths and polycores. Clones producing polysheaths and polycores can be detected electron microscopically only, since these defective particles are biologically inactive.", "contents": "Effective selection of Proteus mirabilis clones producing mirabilicin D-52. From a defective-lysogenic Proteus mirabilis strain we isolated several clones differing in the pattern of their growth on agar plates. Using electron microscopy we have shown some of the selected clones to be efficient in producing mirabilicin D-52 after UV induction, while other clones produced defective mirabilicin polysheaths and polycores. Clones producing polysheaths and polycores can be detected electron microscopically only, since these defective particles are biologically inactive."}
{"id": "PMID:1100208", "title": "Isolation and characterization of a bacteriophage infective for a UDP-galactose-4-epimeraseless mutant of Escherichia coli.", "content": "A DNA bacteriophage, designated CP13, was isolated against Escherichia coli J5, a UDP-galactose-4-epimeraseless mutant of E. coli 0111:B4. Bacteriophage CP13 appears to be specific for rough bacterial strains. Adsorption studies with E. coli J5 grown with galactose show that the bacteriophage will not adsorb when complete lipopolysaccharide is present in the cell membrane. This indicates that lipopolysaccharide may be directly or indirectly involved with the receptor site for bacteriophage CP13. The bacteriophage DNA has a G + C content of 52%.", "contents": "Isolation and characterization of a bacteriophage infective for a UDP-galactose-4-epimeraseless mutant of Escherichia coli. A DNA bacteriophage, designated CP13, was isolated against Escherichia coli J5, a UDP-galactose-4-epimeraseless mutant of E. coli 0111:B4. Bacteriophage CP13 appears to be specific for rough bacterial strains. Adsorption studies with E. coli J5 grown with galactose show that the bacteriophage will not adsorb when complete lipopolysaccharide is present in the cell membrane. This indicates that lipopolysaccharide may be directly or indirectly involved with the receptor site for bacteriophage CP13. The bacteriophage DNA has a G + C content of 52%."}
{"id": "PMID:1100209", "title": "Purification and properties of a nuclear polyhedrosis virus from Choristoneura fumiferana.", "content": "The polyhedral inclusion bodies of a nuclear polyhedrosis virus from the spruce budworm Choristoneura fumiferana were purified by fluorocarbon treatment, sucrose columns, sucrose density gradients, and equilibrium centrifugation. The preparation yields a single and homogeneous band in potassium tartrate gradients at a density of 1.193 g/ml. The virions were released from the polyhedra by alkali treatment and purified by either rate zonal or equilibrium centrifugation in sucrose gradients. The purified virions, which band at a density of 1.265-1.300 g/ml in sucrose, were infectious to the spruce budworm larvae and their mean LD50 was 1.09 +/- 0.1 mug of virus. However, viral nucleocapsids, released by a nonionic detergent, Nonidet P-40, had a density of 1.315 g/ml and were noninfectious by either per os inoculation or intrahemocoelic injection.", "contents": "Purification and properties of a nuclear polyhedrosis virus from Choristoneura fumiferana. The polyhedral inclusion bodies of a nuclear polyhedrosis virus from the spruce budworm Choristoneura fumiferana were purified by fluorocarbon treatment, sucrose columns, sucrose density gradients, and equilibrium centrifugation. The preparation yields a single and homogeneous band in potassium tartrate gradients at a density of 1.193 g/ml. The virions were released from the polyhedra by alkali treatment and purified by either rate zonal or equilibrium centrifugation in sucrose gradients. The purified virions, which band at a density of 1.265-1.300 g/ml in sucrose, were infectious to the spruce budworm larvae and their mean LD50 was 1.09 +/- 0.1 mug of virus. However, viral nucleocapsids, released by a nonionic detergent, Nonidet P-40, had a density of 1.315 g/ml and were noninfectious by either per os inoculation or intrahemocoelic injection."}
{"id": "PMID:1100210", "title": "[Study of the stability and the degradation of fluorescence in the immunofluorescence technic (FTA-ABS) using pulsed lasers].", "content": "The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to quantify exactly the fluorescent signal or repeat the analysis. Stability of fluorescence can be achieved by very short excitation pulses (in the order of a microsecond) and this fast fluorescent signal can be detected only by an electronic detection and recording system. With such a system, high precision is obtained by elimination of errors by the human eye.", "contents": "[Study of the stability and the degradation of fluorescence in the immunofluorescence technic (FTA-ABS) using pulsed lasers]. The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to quantify exactly the fluorescent signal or repeat the analysis. Stability of fluorescence can be achieved by very short excitation pulses (in the order of a microsecond) and this fast fluorescent signal can be detected only by an electronic detection and recording system. With such a system, high precision is obtained by elimination of errors by the human eye."}
{"id": "PMID:1100211", "title": "Repair of heat injury in thymine auxotrophs of Escherichia coli.", "content": "Heat-induced damage in four strains of bacteria has been studied. It has been shown that the damage is repairable on incubation at 37 degrees C in a medium not supporting growth. The extent of repair is dependent upon the strain of bacterium chosen. Recovery from the damage is prominent in the case of filament-forming bacteria, and in non-filament formers, recovery is not totally absent.", "contents": "Repair of heat injury in thymine auxotrophs of Escherichia coli. Heat-induced damage in four strains of bacteria has been studied. It has been shown that the damage is repairable on incubation at 37 degrees C in a medium not supporting growth. The extent of repair is dependent upon the strain of bacterium chosen. Recovery from the damage is prominent in the case of filament-forming bacteria, and in non-filament formers, recovery is not totally absent."}
{"id": "PMID:1100213", "title": "Alpha methyldopahydrazine as an adjunct to levodopa therapy in Parkinson's disease.", "content": "A double-blind, double-observer study was carried out in twenty-five patients with Parkinson's disease. Alpha methyldopahydrazine in combination with L-dopa was compared to placebo with L-dopa. Combination therapy resulted in a reduction in L-dopa dosage to 1/3 of the amount required during the baseline. There were no side effects attributed directly to the alpha methyldopahydrazine. The overall incidence of side effects in the two groups was similar but the combination therapy significantly reduced the incidence of nausea and vomiting. The limiting factor in the combination therapy was the presence of L-dopa induced dyskinesias.", "contents": "Alpha methyldopahydrazine as an adjunct to levodopa therapy in Parkinson's disease. A double-blind, double-observer study was carried out in twenty-five patients with Parkinson's disease. Alpha methyldopahydrazine in combination with L-dopa was compared to placebo with L-dopa. Combination therapy resulted in a reduction in L-dopa dosage to 1/3 of the amount required during the baseline. There were no side effects attributed directly to the alpha methyldopahydrazine. The overall incidence of side effects in the two groups was similar but the combination therapy significantly reduced the incidence of nausea and vomiting. The limiting factor in the combination therapy was the presence of L-dopa induced dyskinesias."}
{"id": "PMID:1100214", "title": "Cucumber shaped and 35 nm particles in Creutzfeldt-Jakob disease.", "content": "A 63 year old female with the ataxic form of Creutzfeldt-Jakob disease (CJD) is presented. In addition to amyloid plaques which were not associated with Alzheimer's neurofibrillary tangles, rare profiles similar to those reported in Scrapie were also seen. To our knowledge, these profiles have never been observed in CJD and their presence in this condition adds a further morphologic similarity between the human and animal forms of subacute spongiform \"viral\" encephalopathies.", "contents": "Cucumber shaped and 35 nm particles in Creutzfeldt-Jakob disease. A 63 year old female with the ataxic form of Creutzfeldt-Jakob disease (CJD) is presented. In addition to amyloid plaques which were not associated with Alzheimer's neurofibrillary tangles, rare profiles similar to those reported in Scrapie were also seen. To our knowledge, these profiles have never been observed in CJD and their presence in this condition adds a further morphologic similarity between the human and animal forms of subacute spongiform \"viral\" encephalopathies."}
{"id": "PMID:1100215", "title": "Choice of gastric drainage procedures.", "content": "The need for drainage after truncal or selective vagotomy is well accepted, but there is no agreement on the most suitable type of drainage procedure. A review of the literature, particularly of papers published in the last 3 years, suggests that whereas vagotomy with gastroenterostomy is followed by a relatively high incidence of bile vomiting, vagotomy with pyloroplasty carries a significantly higher risk of recurrent ulceration. The surgeon, therefore, should not hesitate to perform gastroenterostomy if the pyloroduodenum is severely scarred. As pyloroplasty is irreversible, gastroenterostomy is probably the drainage procedure of choice.", "contents": "Choice of gastric drainage procedures. The need for drainage after truncal or selective vagotomy is well accepted, but there is no agreement on the most suitable type of drainage procedure. A review of the literature, particularly of papers published in the last 3 years, suggests that whereas vagotomy with gastroenterostomy is followed by a relatively high incidence of bile vomiting, vagotomy with pyloroplasty carries a significantly higher risk of recurrent ulceration. The surgeon, therefore, should not hesitate to perform gastroenterostomy if the pyloroduodenum is severely scarred. As pyloroplasty is irreversible, gastroenterostomy is probably the drainage procedure of choice."}
{"id": "PMID:1100220", "title": "Combined cyclophosphamide vincristine, procarbazine, and prednisone (COPP) therapy of malignant lymphoma. Evaluation of 190 patients.", "content": "One hundred ninety patients who had advanced active Hodgkin's disease, lymphosarcoma, or reticulum cell sarcoma were treated with a combination of cyclophosphamide, vincristine, procarbazine, and prednisone (COPP) given in a cyclical fashion every month. Complete remission was produced in 91 of 138 (66%) patients with Hodgkin's disease and in 39 of 52 (75%) patients with non-Hodgkin's lymphoma (lymphosarcoma and reticulum cell sarcoma). The response rate was higher in patients who completed six cycles of therapy compared to those who completed only three to five cycles: 77% vs. 45%, respectively, in Hodgkin's disease, and 85% vs. 46%, respectively, in non-Hodgkin's lymphoma. The median duration of remission was longer for Hodgkin's disease patients who completed six cycles (30 months vs. 10 months). The median duration of complete remission of non-Hodgkin's lymphoma was 14 months. The response to treatment correlated positively with survival. The median survival time start of COPP treatment for patients with Hodgkin's disease was 7 months for nonresponders, 14 months for those who attained partial remission, and more than 48 months for those who attained complete remission. For patients with non-Hodgkin's lymphoma, the median survival time from start of COPP treatment was 24 months for nonresponders and those who had partial remission, and more than 32 months for those who attained complete remission. Of complete remission responders with Hodgkin's disease, 70% are still alive 84 months after diagnosis, and 63% of the patients witn non-Hodgkin's lymphoma are still alive 48 months after diagnosis.", "contents": "Combined cyclophosphamide vincristine, procarbazine, and prednisone (COPP) therapy of malignant lymphoma. Evaluation of 190 patients. One hundred ninety patients who had advanced active Hodgkin's disease, lymphosarcoma, or reticulum cell sarcoma were treated with a combination of cyclophosphamide, vincristine, procarbazine, and prednisone (COPP) given in a cyclical fashion every month. Complete remission was produced in 91 of 138 (66%) patients with Hodgkin's disease and in 39 of 52 (75%) patients with non-Hodgkin's lymphoma (lymphosarcoma and reticulum cell sarcoma). The response rate was higher in patients who completed six cycles of therapy compared to those who completed only three to five cycles: 77% vs. 45%, respectively, in Hodgkin's disease, and 85% vs. 46%, respectively, in non-Hodgkin's lymphoma. The median duration of remission was longer for Hodgkin's disease patients who completed six cycles (30 months vs. 10 months). The median duration of complete remission of non-Hodgkin's lymphoma was 14 months. The response to treatment correlated positively with survival. The median survival time start of COPP treatment for patients with Hodgkin's disease was 7 months for nonresponders, 14 months for those who attained partial remission, and more than 48 months for those who attained complete remission. For patients with non-Hodgkin's lymphoma, the median survival time from start of COPP treatment was 24 months for nonresponders and those who had partial remission, and more than 32 months for those who attained complete remission. Of complete remission responders with Hodgkin's disease, 70% are still alive 84 months after diagnosis, and 63% of the patients witn non-Hodgkin's lymphoma are still alive 48 months after diagnosis."}
{"id": "PMID:1100221", "title": "Clinical comparison of the nitrosoureas.", "content": "The nitrosourea group of antitumor agents was developed by the Division of Cancer Treatment of the National Cancer Institute. Three nitrosoureas (BCNU, CCNU, MeCCNU) have undergone extensive clinical trials, and two of them (BCNU, CCNU) will soon become commercially available. This paper briefly considers the preclinical development of the nitrosoureas and provides an overview and comparison of the extensive clinical data. Information is given regarding any clinical differences. We discuss general conclusions drawn from the nitrosourea development.", "contents": "Clinical comparison of the nitrosoureas. The nitrosourea group of antitumor agents was developed by the Division of Cancer Treatment of the National Cancer Institute. Three nitrosoureas (BCNU, CCNU, MeCCNU) have undergone extensive clinical trials, and two of them (BCNU, CCNU) will soon become commercially available. This paper briefly considers the preclinical development of the nitrosoureas and provides an overview and comparison of the extensive clinical data. Information is given regarding any clinical differences. We discuss general conclusions drawn from the nitrosourea development."}
{"id": "PMID:1100222", "title": "In vitro determination of tritiated thymidine labeling index (LI). Evaluation of a method utilizing hyperbaric oxygen and observations on the LI of human mammary carcinoma.", "content": "A simple method for incubation of tissue specimens with tritiated thymidine under 3 atm oxygen tension gave an in vitro labeling index (LI) that corresponded closely with LI's determined by injection of tritiated thymidine in vivo.  The LI of tumors was not affected by storage at room temperature for up to 135 minutes. The mean LI for 39 human mammary carcinomas was 3.8, but the distribution was skewed to the right, and the most frequently observed LI's were between 2 and 3. Larger carcinomas had significantly larger LI's than smaller tumors, which suggests that the larger tumors were faster-growing at the time of observation than the smaller tumors. The capacity of the larger tumors to maintain a relatively rapid growth rate may account for their large size. The data suggested, but did not prove, that a high LI was associated with more frequent metastasis to axillary lymph nodes.", "contents": "In vitro determination of tritiated thymidine labeling index (LI). Evaluation of a method utilizing hyperbaric oxygen and observations on the LI of human mammary carcinoma. A simple method for incubation of tissue specimens with tritiated thymidine under 3 atm oxygen tension gave an in vitro labeling index (LI) that corresponded closely with LI's determined by injection of tritiated thymidine in vivo.  The LI of tumors was not affected by storage at room temperature for up to 135 minutes. The mean LI for 39 human mammary carcinomas was 3.8, but the distribution was skewed to the right, and the most frequently observed LI's were between 2 and 3. Larger carcinomas had significantly larger LI's than smaller tumors, which suggests that the larger tumors were faster-growing at the time of observation than the smaller tumors. The capacity of the larger tumors to maintain a relatively rapid growth rate may account for their large size. The data suggested, but did not prove, that a high LI was associated with more frequent metastasis to axillary lymph nodes."}
{"id": "PMID:1100223", "title": "Acquired hypertrichosis languiginosa. Report of two new cases and a review of the literature.", "content": "Hypertrichosis lanuginosa is a pathologic state characterized by an excessive, new growth of fine, fetal hair. Two cases of hypertrichosis languinosa with malignancy (lymphoma and uterine cancer) are presented and added to the 9 in the literature. Lymphoma and uterine cancer are previously unreported as associated with hypertrichosis osis lanuginosa. Review of the 11 cases of hypertrichosis lanuginosa revealed the following characteristics: females were predominant; none was below the 4th decade; all had advanced neoplastic disease; all malignancies except one were of epithelial origin; and there were no demonstrable endocrine abnormalities. Despite an attempt to find etiologic factors in our patients and in the literature, none could be elicited.", "contents": "Acquired hypertrichosis languiginosa. Report of two new cases and a review of the literature. Hypertrichosis lanuginosa is a pathologic state characterized by an excessive, new growth of fine, fetal hair. Two cases of hypertrichosis languinosa with malignancy (lymphoma and uterine cancer) are presented and added to the 9 in the literature. Lymphoma and uterine cancer are previously unreported as associated with hypertrichosis osis lanuginosa. Review of the 11 cases of hypertrichosis lanuginosa revealed the following characteristics: females were predominant; none was below the 4th decade; all had advanced neoplastic disease; all malignancies except one were of epithelial origin; and there were no demonstrable endocrine abnormalities. Despite an attempt to find etiologic factors in our patients and in the literature, none could be elicited."}
{"id": "PMID:1100224", "title": "Clinical study of the new podophyllotoxin derivative, 4'-demethylepipodophyllotoxin 9-(4,6-o-ethylidene- beta-D-glucopyranoside) (NSC-141540; VP-16-213), in solid tumors.", "content": "The new semisynthetic epipodophyllotoxin, 4'-demethylepipodophyllotoxin 9-(4,6-o-ethylidene- beta-D-glucopyranoside) (NSC-141540), was tested for antitumor activity against solid tumors and for clinical toxicity in 30 patients. The first two courses were given intravenously (60 mg/m2/day times 5, every 21 days), and subsequent courses were given orally (60-120 mg/m2/day times 5, every 21 days). The drug was subjectively well tolerated but induced considerable leukothrombocytopenia and alopecia. It demonstrated significant activity in oat cell carcinoma of the lung (eight responses out of 11 patients) and ovarian cancer (-our responses out of six patients). NSC-141540 has valuable cytostatic activity against these two tumors and warrants further clinical trials, especially in combination chemotherapy.", "contents": "Clinical study of the new podophyllotoxin derivative, 4'-demethylepipodophyllotoxin 9-(4,6-o-ethylidene- beta-D-glucopyranoside) (NSC-141540; VP-16-213), in solid tumors. The new semisynthetic epipodophyllotoxin, 4'-demethylepipodophyllotoxin 9-(4,6-o-ethylidene- beta-D-glucopyranoside) (NSC-141540), was tested for antitumor activity against solid tumors and for clinical toxicity in 30 patients. The first two courses were given intravenously (60 mg/m2/day times 5, every 21 days), and subsequent courses were given orally (60-120 mg/m2/day times 5, every 21 days). The drug was subjectively well tolerated but induced considerable leukothrombocytopenia and alopecia. It demonstrated significant activity in oat cell carcinoma of the lung (eight responses out of 11 patients) and ovarian cancer (-our responses out of six patients). NSC-141540 has valuable cytostatic activity against these two tumors and warrants further clinical trials, especially in combination chemotherapy."}
{"id": "PMID:1100225", "title": "4'-demethylepipodophyllotoxin 9-(4,6-o-2-thenylidene-beta-D-glucopyranoside) (NSC-122819; VM-26) and 4'-demethylepipodophyllotoxin 9-(4.6-0-ethylidene-beta-D-glucopyranoside) (NSC-141540; VP-16-213) in childhood cancer: preliminary observations.", "content": "We evaluated the responses of 39 children with cancer who, after failure to respond to conventional chemotherapeutic agents, received either or both of two epipodophyllotoxins: 4'-demethylepipodophyllotoxin 9-(4,6-o-2-thenylidene-beta-D-glucopyranoside) (NSC-122819) and 4'-demethylepipodophyllotoxin 9-(4,6-o-ethylidene-beta-D-glucopyranoside) (NSC-141540). Seventeen patients has acute lymphocytic leukemia (ALL). 12 had acute nonlymphocytic leukemia (ANLL), and ten had solid tumors. Initially, the patients in each disease category were randomized to receive 50 mg/m2/dose of NSC-122819 intravenously (iv) twice weekly or 75 mg/m2/dose iv of NSC-141540 twice weekly for 4 weeks. Drug dosages and schedules of administration were adjusted during the course of the study. Although objective responses were not detected in the heterogeneous group of solid tumor patients, definite clinical responses were obtained in nine of the 29 patients with acute leukemia. The responses to the two epipodophyllotoxins were noted in patients with ALL as well as in patients with ANLL. Toxic side-effects included nausea, vomiting, diarrhea, fever, alopecia, leukopenia, and thrombocytopenia. These results, the first reported with both NSC-122819 and NSC-141540 in childhood cancer, indicate that the epipodophyllotoxins are well tolerated and may be effective against acute leukemia.", "contents": "4'-demethylepipodophyllotoxin 9-(4,6-o-2-thenylidene-beta-D-glucopyranoside) (NSC-122819; VM-26) and 4'-demethylepipodophyllotoxin 9-(4.6-0-ethylidene-beta-D-glucopyranoside) (NSC-141540; VP-16-213) in childhood cancer: preliminary observations. We evaluated the responses of 39 children with cancer who, after failure to respond to conventional chemotherapeutic agents, received either or both of two epipodophyllotoxins: 4'-demethylepipodophyllotoxin 9-(4,6-o-2-thenylidene-beta-D-glucopyranoside) (NSC-122819) and 4'-demethylepipodophyllotoxin 9-(4,6-o-ethylidene-beta-D-glucopyranoside) (NSC-141540). Seventeen patients has acute lymphocytic leukemia (ALL). 12 had acute nonlymphocytic leukemia (ANLL), and ten had solid tumors. Initially, the patients in each disease category were randomized to receive 50 mg/m2/dose of NSC-122819 intravenously (iv) twice weekly or 75 mg/m2/dose iv of NSC-141540 twice weekly for 4 weeks. Drug dosages and schedules of administration were adjusted during the course of the study. Although objective responses were not detected in the heterogeneous group of solid tumor patients, definite clinical responses were obtained in nine of the 29 patients with acute leukemia. The responses to the two epipodophyllotoxins were noted in patients with ALL as well as in patients with ANLL. Toxic side-effects included nausea, vomiting, diarrhea, fever, alopecia, leukopenia, and thrombocytopenia. These results, the first reported with both NSC-122819 and NSC-141540 in childhood cancer, indicate that the epipodophyllotoxins are well tolerated and may be effective against acute leukemia."}
{"id": "PMID:1100226", "title": "Phase I clinical trial of isophosphamide (NSC-109724).", "content": "An initial clinical phase I trial of isophosphamide has been carried out at dose levels of 200-10,000 mg/m2 of body surface area using a single-dose, every-3-week schedule. Significant toxicity was not seen at isophosphamide dose levels less than 2900 mg/m2. At higher doses, nausea and vomiting was nearly universal. Hematologic toxicity manifested by leukopenia occurred in 12 of 20 patients treated with doses of 3800-7000 mg/m2. Thrombocytopenia to 100,000 platelets/mm3 was not seen in any patient. Urinary bladder toxicity manifested by hemorrhagic cystitis was seen in 15 of 23 patients treated with doses of 3800-10,000 mg/m2. Hemorrhagic cystitis could be completely prevented by bladder irrigation with N-acetyl-L-cysteine (1% solution, 2000 ml/day). With careful attention to hydration, renal toxicity was largely prevented with blood urea nitrogen elevations to 30 mg/dl in only two of 14 patients receiving isophosphamide doses of 5000-7000 mg/m2. Another side effect was central nervous system toxicity in seven of 17 patients at doses of 5000-10,000 mg/m2. For phase II trials a dose of 5000 mg/m2 is recommended, with careful attention to the state of hydration in the patient and with the knowledge that bladder irrigation may be necessary for the prevention and/or amelioration of bladder toxicity.", "contents": "Phase I clinical trial of isophosphamide (NSC-109724). An initial clinical phase I trial of isophosphamide has been carried out at dose levels of 200-10,000 mg/m2 of body surface area using a single-dose, every-3-week schedule. Significant toxicity was not seen at isophosphamide dose levels less than 2900 mg/m2. At higher doses, nausea and vomiting was nearly universal. Hematologic toxicity manifested by leukopenia occurred in 12 of 20 patients treated with doses of 3800-7000 mg/m2. Thrombocytopenia to 100,000 platelets/mm3 was not seen in any patient. Urinary bladder toxicity manifested by hemorrhagic cystitis was seen in 15 of 23 patients treated with doses of 3800-10,000 mg/m2. Hemorrhagic cystitis could be completely prevented by bladder irrigation with N-acetyl-L-cysteine (1% solution, 2000 ml/day). With careful attention to hydration, renal toxicity was largely prevented with blood urea nitrogen elevations to 30 mg/dl in only two of 14 patients receiving isophosphamide doses of 5000-7000 mg/m2. Another side effect was central nervous system toxicity in seven of 17 patients at doses of 5000-10,000 mg/m2. For phase II trials a dose of 5000 mg/m2 is recommended, with careful attention to the state of hydration in the patient and with the knowledge that bladder irrigation may be necessary for the prevention and/or amelioration of bladder toxicity."}
{"id": "PMID:1100233", "title": "Ambulatory monitoring of arrhythmias.", "content": "Currently available instrumentation provides the capability of ambulatory electrocardiographic monitoring. A considerable body of literature has developed since the late 1950s which defines an important role for this technique in clinical practice. The use of ambulatory monitoring may ultimately prove as useful in the diagnosis and treatment of occult arrhythmias during activity as monitoring has been during acute phases of myocardial infarction.", "contents": "Ambulatory monitoring of arrhythmias. Currently available instrumentation provides the capability of ambulatory electrocardiographic monitoring. A considerable body of literature has developed since the late 1950s which defines an important role for this technique in clinical practice. The use of ambulatory monitoring may ultimately prove as useful in the diagnosis and treatment of occult arrhythmias during activity as monitoring has been during acute phases of myocardial infarction."}
{"id": "PMID:1100236", "title": "Echocardiography in congenital heart disease.", "content": "It has been demonstrated that echocardiography is a useful and accurate noninvasive technique for the study of congenital cardiovascular diseases. This method provides significant information in that invasive cardiac catheterization and angiocardiography can be planned with greater facility. This is especially important in severely ill babies in whom invasive diagnostic methods are risky. It is particularly attractive in neonates in whom the clinical differentiation of lethal heart disease from septic shock is difficult, since in the latter situation the echographic findings are normal.", "contents": "Echocardiography in congenital heart disease. It has been demonstrated that echocardiography is a useful and accurate noninvasive technique for the study of congenital cardiovascular diseases. This method provides significant information in that invasive cardiac catheterization and angiocardiography can be planned with greater facility. This is especially important in severely ill babies in whom invasive diagnostic methods are risky. It is particularly attractive in neonates in whom the clinical differentiation of lethal heart disease from septic shock is difficult, since in the latter situation the echographic findings are normal."}
{"id": "PMID:1100237", "title": "Diagnostic value of systolic time intervals in man.", "content": "The STI offer a quantitative noninvasive measure of left ventricular performance in man. Extensive studies in animals and man have validated the STI. The STI are also sensitive to changes in inotropic state, preload, and afterload, and distinction must be made between the differing effects of acute and chronic alterations upon the STI. When properly performed and interpreted the STI are useful in diagnosis (aortic valve disease, angina pectoris, and pericardial disease), evaluation of the effectiveness of cardiac compensation (coronary artery disease, mitral valve disease, hypertensive heart disease), and evaluation of surgical or pharmacologic interventions. While much investigation is required for a more comprehensive understanding of the clinical application of the STI, the studies summarized in this review support continued use of these measures for the evaluation of left ventricular performance in man.", "contents": "Diagnostic value of systolic time intervals in man. The STI offer a quantitative noninvasive measure of left ventricular performance in man. Extensive studies in animals and man have validated the STI. The STI are also sensitive to changes in inotropic state, preload, and afterload, and distinction must be made between the differing effects of acute and chronic alterations upon the STI. When properly performed and interpreted the STI are useful in diagnosis (aortic valve disease, angina pectoris, and pericardial disease), evaluation of the effectiveness of cardiac compensation (coronary artery disease, mitral valve disease, hypertensive heart disease), and evaluation of surgical or pharmacologic interventions. While much investigation is required for a more comprehensive understanding of the clinical application of the STI, the studies summarized in this review support continued use of these measures for the evaluation of left ventricular performance in man."}
{"id": "PMID:1100239", "title": "Coronary arteriography: indications and pitfalls.", "content": "Selective coronary arteriography has become an unusually useful technique in evaluating patients with known or suspected ischemic heart disease. The contribution of F. Mason Sones in developing the technique and in emphasizing that properly performed studies in experienced hands can be done at an acceptable risk cannot be overemphasized. Although the study can give information only on the anatomical aspects of the disease, it has remarkably broadened ouo understanding of the whole spectrum of coronary arteriosclerosis and ischemic heart disease. This is related to the fact that the method has provided the opportunity to study an extraordinarily large number of patients representing virtually every clinical subset of the disease. The importance of nonanatomical factors, better prognostic information about the disease, and more complete understanding of the hemodynamic ocrrelates and mechanisms of drug action have resulted from investigative studies using coronary arteriographic methods. Because ischemic heart disease is such a complex and variable entity, it is important to stress that coronary arteriography and left ventricular angiography can provide only anatomical information, and that the method has significant but ordinarily recognizable limitations. Some of these limitations are technical and are not important considerations in experienced hands in well-run laboratories. Other problems are inherent in the anatomical complexity of the disease and the coronary circulation. Hence, appropriate emphasis on the details of the patient's history, including risk factors and age, as well as other selected clinical laboratory studiies, such as exercise electrocardiography, are important in assessing the patient's total problem. Because of these factors, the greatest value of coronary arteriography is realized when it is performed as part of a thoughtful, detailed--and highly individualized--evaluation of the patient.", "contents": "Coronary arteriography: indications and pitfalls. Selective coronary arteriography has become an unusually useful technique in evaluating patients with known or suspected ischemic heart disease. The contribution of F. Mason Sones in developing the technique and in emphasizing that properly performed studies in experienced hands can be done at an acceptable risk cannot be overemphasized. Although the study can give information only on the anatomical aspects of the disease, it has remarkably broadened ouo understanding of the whole spectrum of coronary arteriosclerosis and ischemic heart disease. This is related to the fact that the method has provided the opportunity to study an extraordinarily large number of patients representing virtually every clinical subset of the disease. The importance of nonanatomical factors, better prognostic information about the disease, and more complete understanding of the hemodynamic ocrrelates and mechanisms of drug action have resulted from investigative studies using coronary arteriographic methods. Because ischemic heart disease is such a complex and variable entity, it is important to stress that coronary arteriography and left ventricular angiography can provide only anatomical information, and that the method has significant but ordinarily recognizable limitations. Some of these limitations are technical and are not important considerations in experienced hands in well-run laboratories. Other problems are inherent in the anatomical complexity of the disease and the coronary circulation. Hence, appropriate emphasis on the details of the patient's history, including risk factors and age, as well as other selected clinical laboratory studiies, such as exercise electrocardiography, are important in assessing the patient's total problem. Because of these factors, the greatest value of coronary arteriography is realized when it is performed as part of a thoughtful, detailed--and highly individualized--evaluation of the patient."}
{"id": "PMID:1100240", "title": "Value of ventriculography in cardiac function and diagnosis.", "content": "The methods for calculating left ventricular volumes employing biplane angiocardiograms and the calculated parameters of left ventricular mass, pressure-volume diagrams, wall forces, compliance, and abnormally contracting segments are described. Examples of these calculations are given and the contributions to the assessment of cardiac function and diagnosis are discussed. Normal values and the alterations produced by volume overload, pressure overload, myocardial disease, and coronary artery disease are presented.", "contents": "Value of ventriculography in cardiac function and diagnosis. The methods for calculating left ventricular volumes employing biplane angiocardiograms and the calculated parameters of left ventricular mass, pressure-volume diagrams, wall forces, compliance, and abnormally contracting segments are described. Examples of these calculations are given and the contributions to the assessment of cardiac function and diagnosis are discussed. Normal values and the alterations produced by volume overload, pressure overload, myocardial disease, and coronary artery disease are presented."}
{"id": "PMID:1100241", "title": "Radioisotopic measurements of coronary blood flow.", "content": "This review has presented a critical evaluation of current methods used in measurement of total and regional blood flow. Measurement of total coronary blood flow furnishes little evidence of pathological processes within the coronary vascular system. However, in conjunction with coronary vasodilators, measurement of total coronary blood flow may indicate the presence of coronary artery disease. Difficulties inherent in the determination of regional coronary blood flow lie primarily in defining clearance slopes and in the presence of accidental counts. More promising are methods using scintigraphs obtained with radioactive particles injected into the coronary artery. However, these procedures do not measure flow, only flow distribution. A procedure for measuring regional uptake of positron emitters by coincidence counting has been described; the method possesses definite advantages over other procedures, since it is noninvasive and appears to have good resolution. However, the method is dependent on higher decay density which necessitates the use of nuclides with a short half-life (e.g., 81rubidium).", "contents": "Radioisotopic measurements of coronary blood flow. This review has presented a critical evaluation of current methods used in measurement of total and regional blood flow. Measurement of total coronary blood flow furnishes little evidence of pathological processes within the coronary vascular system. However, in conjunction with coronary vasodilators, measurement of total coronary blood flow may indicate the presence of coronary artery disease. Difficulties inherent in the determination of regional coronary blood flow lie primarily in defining clearance slopes and in the presence of accidental counts. More promising are methods using scintigraphs obtained with radioactive particles injected into the coronary artery. However, these procedures do not measure flow, only flow distribution. A procedure for measuring regional uptake of positron emitters by coincidence counting has been described; the method possesses definite advantages over other procedures, since it is noninvasive and appears to have good resolution. However, the method is dependent on higher decay density which necessitates the use of nuclides with a short half-life (e.g., 81rubidium)."}
{"id": "PMID:1100243", "title": "Myocardial enzymes.", "content": "1. The use of enzyme analysis has proved considerably valuable in the diagnosis of myocardial infarction. 2. Serial studies of multiple enzymes appear to lend greater specificity and sensitivity to such testing. 3. Creatine phosphokinase appears to be the most useful of the conventional enzymes employed in the diagnosis of myocardial infarction, but limitations due to technical factors and other clinical states causing elevation must be recognized. 4. Current studies to enhance the specificity of enzyme diagnosis in myocardial infarction include the employment of isoenzymes of CPK and LDH. 5. Enzymes appear to afford significant benefit in assessing the prognosis following myocardial infarction and may aid in the selection of therapy.", "contents": "Myocardial enzymes. 1. The use of enzyme analysis has proved considerably valuable in the diagnosis of myocardial infarction. 2. Serial studies of multiple enzymes appear to lend greater specificity and sensitivity to such testing. 3. Creatine phosphokinase appears to be the most useful of the conventional enzymes employed in the diagnosis of myocardial infarction, but limitations due to technical factors and other clinical states causing elevation must be recognized. 4. Current studies to enhance the specificity of enzyme diagnosis in myocardial infarction include the employment of isoenzymes of CPK and LDH. 5. Enzymes appear to afford significant benefit in assessing the prognosis following myocardial infarction and may aid in the selection of therapy."}
{"id": "PMID:1100245", "title": "Transcutaneous Doppler detector in the study of arterial and venous flow patterns.", "content": "The transcutaneous Doppler flow detector is not presently suited for volume quantitation. However, the arterial flow velocity profile has been established as a valuable procedure for the diagnosis of peripheral arterial disease and assessment of abnormalities of peripheral flow that may result from cardiac arrhythmia or cardiac dysfunction. Distinctive, diagnostic arterial flow patterns in patients with hypertrophic subaortic stenosis and in patients with aortic insufficiency have been described. Occlusive venous disease in the popliteal vein and more proximal vessels can be diagnopsed with an accuracy that justifies the use of the Doppler ultrasound examination as a screening procedure in individuals subject to thrombophlebitis. It is reasonable to anticipate that future work with pulsed Doppler instruments will establish this technique of imaging as a practical clinical method for noninvasive arteriography.", "contents": "Transcutaneous Doppler detector in the study of arterial and venous flow patterns. The transcutaneous Doppler flow detector is not presently suited for volume quantitation. However, the arterial flow velocity profile has been established as a valuable procedure for the diagnosis of peripheral arterial disease and assessment of abnormalities of peripheral flow that may result from cardiac arrhythmia or cardiac dysfunction. Distinctive, diagnostic arterial flow patterns in patients with hypertrophic subaortic stenosis and in patients with aortic insufficiency have been described. Occlusive venous disease in the popliteal vein and more proximal vessels can be diagnopsed with an accuracy that justifies the use of the Doppler ultrasound examination as a screening procedure in individuals subject to thrombophlebitis. It is reasonable to anticipate that future work with pulsed Doppler instruments will establish this technique of imaging as a practical clinical method for noninvasive arteriography."}
{"id": "PMID:1100246", "title": "Diagnostic uses of electrical pacing.", "content": "Diagnostic pacing has proven useful for the study of a great variety of clinical problems. Rapid atrial pacing is an excellent means of stressing the heart, particularly in patients with ischemic heart disease. Pacing-induced tachycardia has been used to provoke typical coronary pain and to produce hemodynamic, metabolic, and left ventricular contractile changes in patients with coronary artery disease. Because this heart stress is reproducible, it has also been valuable in assessing response to medical and surgic al therapy in patients with angina. Electrophysiologically, pacing has been used to clarify mechanisms of normal and abnormal function of the sinus node and A-V conduction. The pre-excitation states have been more precisely defined, and the introduction of programmed electrical stimuli into the cardiac cycle has helped elucidate the nature of re-entry supraventricular tachycardias.", "contents": "Diagnostic uses of electrical pacing. Diagnostic pacing has proven useful for the study of a great variety of clinical problems. Rapid atrial pacing is an excellent means of stressing the heart, particularly in patients with ischemic heart disease. Pacing-induced tachycardia has been used to provoke typical coronary pain and to produce hemodynamic, metabolic, and left ventricular contractile changes in patients with coronary artery disease. Because this heart stress is reproducible, it has also been valuable in assessing response to medical and surgic al therapy in patients with angina. Electrophysiologically, pacing has been used to clarify mechanisms of normal and abnormal function of the sinus node and A-V conduction. The pre-excitation states have been more precisely defined, and the introduction of programmed electrical stimuli into the cardiac cycle has helped elucidate the nature of re-entry supraventricular tachycardias."}
{"id": "PMID:1100247", "title": "Usefulness and limitations of precordial phonocardiography and external pulse recordings.", "content": "Phonocardiography provides one of a growing list of valuable intermediary studies between physical examination and cardiac catheterization. It not only provides a medium for instruction in physical examination, but also allows one to make certain diagnoses and assess severity of numerous cardiovascular lesions. It is especially valuable in the evaluation of all valvular stenoses, especially aortic stenosis. One can also use phonocardiography for screening and diagnosis of idiopathic hypertrophic subaortic stenosis. It is a helpful adjunct in the study of several congenital abnormalities as well as in the assessment of myocardial function in cardiomyopathies and ischemic heart disease, and may often be decisive in confirming the diagnosis of pericardial constriction.", "contents": "Usefulness and limitations of precordial phonocardiography and external pulse recordings. Phonocardiography provides one of a growing list of valuable intermediary studies between physical examination and cardiac catheterization. It not only provides a medium for instruction in physical examination, but also allows one to make certain diagnoses and assess severity of numerous cardiovascular lesions. It is especially valuable in the evaluation of all valvular stenoses, especially aortic stenosis. One can also use phonocardiography for screening and diagnosis of idiopathic hypertrophic subaortic stenosis. It is a helpful adjunct in the study of several congenital abnormalities as well as in the assessment of myocardial function in cardiomyopathies and ischemic heart disease, and may often be decisive in confirming the diagnosis of pericardial constriction."}
{"id": "PMID:1100250", "title": "Followup of implanted pacemakers: an evaluation of surveillance methods.", "content": "A waveform analysis clinic augmented by telephone transmission of pacemaker interval in the late stages of pacemaker life will yield a considerable amount of information and will permit elective replacement of pacemakers in about 90 per cent of cases. The clinic has an advantage over other surveillance systems in the accuracy of the diagnosis, the identification of abnormalities that do not require pacemaker replacement, and the multiple benefits of a direct doctor-patient relationship. No system of surveillance can be recommended over all others in all circumstances. It is sufficient here to indicate the merits of each system and to allow the various centers to develop according to their own particular needs and desires. There is no objection to telephone monitoring alone as long as one realizes that only about 80 per cent of the problems can be detected and that there will be an irreducible percentage of false negative and false positive diagnoses. The danger of errors of this type is not great, but it does exist and should be avoided if possible. Other methods of pacemaker followup, such as simple examination and an electrocardiogram in a doctor's office or changing the pacemaker on the basis of the manufacturer's prediction, are relatively unsatisfactory. It should be stressed that pacemaker surveillance of some type is essential to satisfactory patient care because it provides for maximum utilization of the pacemaker, for replacement only if and when necessary, for detection of 90 per cent of pacemaker problems, and for protection of the patient against unexpected pacemaker failure.", "contents": "Followup of implanted pacemakers: an evaluation of surveillance methods. A waveform analysis clinic augmented by telephone transmission of pacemaker interval in the late stages of pacemaker life will yield a considerable amount of information and will permit elective replacement of pacemakers in about 90 per cent of cases. The clinic has an advantage over other surveillance systems in the accuracy of the diagnosis, the identification of abnormalities that do not require pacemaker replacement, and the multiple benefits of a direct doctor-patient relationship. No system of surveillance can be recommended over all others in all circumstances. It is sufficient here to indicate the merits of each system and to allow the various centers to develop according to their own particular needs and desires. There is no objection to telephone monitoring alone as long as one realizes that only about 80 per cent of the problems can be detected and that there will be an irreducible percentage of false negative and false positive diagnoses. The danger of errors of this type is not great, but it does exist and should be avoided if possible. Other methods of pacemaker followup, such as simple examination and an electrocardiogram in a doctor's office or changing the pacemaker on the basis of the manufacturer's prediction, are relatively unsatisfactory. It should be stressed that pacemaker surveillance of some type is essential to satisfactory patient care because it provides for maximum utilization of the pacemaker, for replacement only if and when necessary, for detection of 90 per cent of pacemaker problems, and for protection of the patient against unexpected pacemaker failure."}
{"id": "PMID:1100251", "title": "Intracardiac sound and pressure: right heart events. Toward an ultimate classification of auscultatory events.", "content": "These observations are presented as a tentative step towards an ultimate classification of auscultatory events based on ICSP studies, chest wall phonocardiography, and auscultatory areas, with continuing clarification or modification by angiography, Doppler techniques, echocardiography, computer usage, and any available, useful technique. The ultimate diagnostic value of ICSP information resides in the feed-back loop wherein this information is used daily at the bedside as part of the understanding and explanation of daily auscultatory phenomena.", "contents": "Intracardiac sound and pressure: right heart events. Toward an ultimate classification of auscultatory events. These observations are presented as a tentative step towards an ultimate classification of auscultatory events based on ICSP studies, chest wall phonocardiography, and auscultatory areas, with continuing clarification or modification by angiography, Doppler techniques, echocardiography, computer usage, and any available, useful technique. The ultimate diagnostic value of ICSP information resides in the feed-back loop wherein this information is used daily at the bedside as part of the understanding and explanation of daily auscultatory phenomena."}
{"id": "PMID:1100259", "title": "Immunohistochemical detection of growth hormone-release inhibiting hormone (somatostatin) in the guinea-pig brain.", "content": "Utilizing the unlabeled antibody peroxidase-antiperoxidase method, we have studied the distribution of somatostatin (growth hormone release-inhibiting hormone) in the guinea pig brain. An immunohistochemical reaction was found in both internal and external zone of the caudal and median portions of the median eminence. Reaction products were also present in the arcuate nucleus in proximity of the third ventricule. The immunostained elements resembled nerve processes. No cell bodies were stained. A positive reaction was also present in the whole organum vasculosum of the lamina terminalis. In the subfornical and subcommissural organs, somatostatin was observed in the ependymal and subependymal layers and the perivascular areas. In the pineal gland, a positive reaction was also found mainly in the perivascular areas. All the other brain areas were completely negative. These results establish that, in the hypothalamus, somatostatin is mainly concentrated in the median eminence and suggest that periventricular organs can play a role in the regulation of growth hormone secretion.", "contents": "Immunohistochemical detection of growth hormone-release inhibiting hormone (somatostatin) in the guinea-pig brain. Utilizing the unlabeled antibody peroxidase-antiperoxidase method, we have studied the distribution of somatostatin (growth hormone release-inhibiting hormone) in the guinea pig brain. An immunohistochemical reaction was found in both internal and external zone of the caudal and median portions of the median eminence. Reaction products were also present in the arcuate nucleus in proximity of the third ventricule. The immunostained elements resembled nerve processes. No cell bodies were stained. A positive reaction was also present in the whole organum vasculosum of the lamina terminalis. In the subfornical and subcommissural organs, somatostatin was observed in the ependymal and subependymal layers and the perivascular areas. In the pineal gland, a positive reaction was also found mainly in the perivascular areas. All the other brain areas were completely negative. These results establish that, in the hypothalamus, somatostatin is mainly concentrated in the median eminence and suggest that periventricular organs can play a role in the regulation of growth hormone secretion."}
{"id": "PMID:1100261", "title": "The ratio of smooth muscle cell volume to cell surface area measured by electron microscopy with corrections for shrinkage.", "content": "The ratio of cell volume to cell surface area (V/A) has been measured by electron microscopy, for smooth muscle cells of the guinea-pig taenia coli. The extent of shrinkage which occurs during processing for electron microscopy has been estimated using Archimedes' principle. The corrected ratio of V/A is 0.8 mum which is lower than previously published estimates for this tissue. The contribution of the surface vesicles to V/A was not included in the present estimate, but the extra surface area contributed by these organelles would lower V/A even more.", "contents": "The ratio of smooth muscle cell volume to cell surface area measured by electron microscopy with corrections for shrinkage. The ratio of cell volume to cell surface area (V/A) has been measured by electron microscopy, for smooth muscle cells of the guinea-pig taenia coli. The extent of shrinkage which occurs during processing for electron microscopy has been estimated using Archimedes' principle. The corrected ratio of V/A is 0.8 mum which is lower than previously published estimates for this tissue. The contribution of the surface vesicles to V/A was not included in the present estimate, but the extra surface area contributed by these organelles would lower V/A even more."}
{"id": "PMID:1100260", "title": "Intracellular localization of actin with fluorescently labelled heavy meromyosin.", "content": "An improved technique for fluorescent labelling of heavy meromyosin has made it possible to detect on a light microscopic level the cellular sites of actin localization. Rabbit heavy meromyosin (HMM) was labelled with fluorescein isothiocyanate so that the actin binding site was protected during the reaction. The specificity of fluorescent HMM binding to cellular actin was tested by using glycerinated myofibrils. Staining was most intense in the I-Bands, and decreased at the edges of the A-Band, where actin filaments overlap with myosin. No staining occurred in the H-Zones or in the Z-Bands. The fluorescent HMM could be removed by washing with a relaxing solution. Similar sarcomeric patterns were obtained when embryonic chick skeletal and cardiac muscle cells were stained with fluorescent HMM. Localized fluorescent staining was also observed in smooth muscle fibers, axons and growth cones of nerves, acrosomal caps of sperm, cleavage furrows of dividing cells and pseudopods of various motile cells, all of which are known to contain actin. In sessile cells, the actin was found predominantly in fibrous bundles. This pattern of actin localization was altered when the cells underwent cleavage or became motile. The relationship between the intracellular distribution of actin and its function in the cell is discussed.", "contents": "Intracellular localization of actin with fluorescently labelled heavy meromyosin. An improved technique for fluorescent labelling of heavy meromyosin has made it possible to detect on a light microscopic level the cellular sites of actin localization. Rabbit heavy meromyosin (HMM) was labelled with fluorescein isothiocyanate so that the actin binding site was protected during the reaction. The specificity of fluorescent HMM binding to cellular actin was tested by using glycerinated myofibrils. Staining was most intense in the I-Bands, and decreased at the edges of the A-Band, where actin filaments overlap with myosin. No staining occurred in the H-Zones or in the Z-Bands. The fluorescent HMM could be removed by washing with a relaxing solution. Similar sarcomeric patterns were obtained when embryonic chick skeletal and cardiac muscle cells were stained with fluorescent HMM. Localized fluorescent staining was also observed in smooth muscle fibers, axons and growth cones of nerves, acrosomal caps of sperm, cleavage furrows of dividing cells and pseudopods of various motile cells, all of which are known to contain actin. In sessile cells, the actin was found predominantly in fibrous bundles. This pattern of actin localization was altered when the cells underwent cleavage or became motile. The relationship between the intracellular distribution of actin and its function in the cell is discussed."}
{"id": "PMID:1100285", "title": "Interaction of water with egg lecithin in benzene solution.", "content": "Benzene solutions of purified egg lecithin, with small amounts of water added, have been examined by 60 MGz and 100 MHz NMR spectroscopy, infrared spectrophotometry and phase contrast microscopy. The transverse relaxation times of the water, N-methyl and O--H protons are dependent on water concentration. This dependence changes sharply for the water proton at a level of one water molecule per lecithin monohydrate molecule. These results do not fully agree with those reported by other workers. Four mathematical models are examined which could account for the behaviour of the water protons. Models which assume a constant transverse relaxation time for water protons above a level of one water molecule per lecithin molecule cannot predict the behaviour observed. It is sufficient to assume that water protons above this concentration have a single relaxation time which is a linear function of water concentration. The added water associates primarily with the phosphate in the lecithin head group. Above nine water molecules per lecithin monohydrate molecule free water is present in the system.", "contents": "Interaction of water with egg lecithin in benzene solution. Benzene solutions of purified egg lecithin, with small amounts of water added, have been examined by 60 MGz and 100 MHz NMR spectroscopy, infrared spectrophotometry and phase contrast microscopy. The transverse relaxation times of the water, N-methyl and O--H protons are dependent on water concentration. This dependence changes sharply for the water proton at a level of one water molecule per lecithin monohydrate molecule. These results do not fully agree with those reported by other workers. Four mathematical models are examined which could account for the behaviour of the water protons. Models which assume a constant transverse relaxation time for water protons above a level of one water molecule per lecithin molecule cannot predict the behaviour observed. It is sufficient to assume that water protons above this concentration have a single relaxation time which is a linear function of water concentration. The added water associates primarily with the phosphate in the lecithin head group. Above nine water molecules per lecithin monohydrate molecule free water is present in the system."}
{"id": "PMID:1100288", "title": "Tissue vitamin B-12 assay by a radioisotope dilution technique.", "content": "A method is described for the measurement of vitamin B-12 (B-12) in solid tissues by radioisotope dilution (RID) assay. The method is a modivication using double extraction of a serum B-12 RID assay which uses chicken serum as the B-12 binder. The method was developed and tested using human and bat liver specimens. Double extraction was shown to be more efficient than single extraction, and resulted in complete release of endogenous liver [57Co] B-12 administered to bats. Results using the RID assay in 16 humans and 17 bat liver specimens were compared with those obtained using the Lactobacillus leichmannii microbiological assay. Correlation was good, but the RID assay gave higher results using the microbiological assay appear to be due to inadequate extraction of B-12 from tissues.", "contents": "Tissue vitamin B-12 assay by a radioisotope dilution technique. A method is described for the measurement of vitamin B-12 (B-12) in solid tissues by radioisotope dilution (RID) assay. The method is a modivication using double extraction of a serum B-12 RID assay which uses chicken serum as the B-12 binder. The method was developed and tested using human and bat liver specimens. Double extraction was shown to be more efficient than single extraction, and resulted in complete release of endogenous liver [57Co] B-12 administered to bats. Results using the RID assay in 16 humans and 17 bat liver specimens were compared with those obtained using the Lactobacillus leichmannii microbiological assay. Correlation was good, but the RID assay gave higher results using the microbiological assay appear to be due to inadequate extraction of B-12 from tissues."}
{"id": "PMID:1100289", "title": "Gonadotrophin hormone releasing tests in women receiving hormonal contraception.", "content": "Gonadotrophin secretion by the pituitary was tested by i.v. injections of synthetic LH and FSH releasing hormone (LHRH) in five normal women between day 21 and day 25 of the luteal phase of the cycle, in twelve women receiving contraception with i.m. injection of medroxyprogesterone acetate and in eight women taking combined oral contraceptives of different varieties. Treatment with oral contraceptives led to a decrease or even to complete suppression of the response and possibly the reserve capacity of both LH and FSH secretions. Treatment with medroxyprogesterone acetate for several years lowered basal serum LH and FSH as did oral contraceptives but had much less effect on the responses to LRH. In long-term steroid contraception, progestogens alone seem to exert much less deleterious effect on gonadotrophin function than when used in combination with an oestrogen.", "contents": "Gonadotrophin hormone releasing tests in women receiving hormonal contraception. Gonadotrophin secretion by the pituitary was tested by i.v. injections of synthetic LH and FSH releasing hormone (LHRH) in five normal women between day 21 and day 25 of the luteal phase of the cycle, in twelve women receiving contraception with i.m. injection of medroxyprogesterone acetate and in eight women taking combined oral contraceptives of different varieties. Treatment with oral contraceptives led to a decrease or even to complete suppression of the response and possibly the reserve capacity of both LH and FSH secretions. Treatment with medroxyprogesterone acetate for several years lowered basal serum LH and FSH as did oral contraceptives but had much less effect on the responses to LRH. In long-term steroid contraception, progestogens alone seem to exert much less deleterious effect on gonadotrophin function than when used in combination with an oestrogen."}
{"id": "PMID:1100290", "title": "The effect of progesterone and FSH and LH response to LHRH in normal women.", "content": "An LHRH test was performed on twenty-eight regularly menstruating females during follicular phase of their cycles. Twenty-four were then given an intramuscular injection of progesterone in oil and retested with LHRH 20 or 44 h later, the remaining four received no pretreatment and acted as controls. The twelve subjects retested 20 h after the progesterone injection showed marked changes in response. The greatest increase in gonadotrophin release after LHRH was seen when subjects were tested between days 8 and 10 of the menstrual cycle. In contrast, the twelve studied 44 h after receiving the steroid showed no change in release.", "contents": "The effect of progesterone and FSH and LH response to LHRH in normal women. An LHRH test was performed on twenty-eight regularly menstruating females during follicular phase of their cycles. Twenty-four were then given an intramuscular injection of progesterone in oil and retested with LHRH 20 or 44 h later, the remaining four received no pretreatment and acted as controls. The twelve subjects retested 20 h after the progesterone injection showed marked changes in response. The greatest increase in gonadotrophin release after LHRH was seen when subjects were tested between days 8 and 10 of the menstrual cycle. In contrast, the twelve studied 44 h after receiving the steroid showed no change in release."}
{"id": "PMID:1100287", "title": "[Duration of action of the pyrimethamine-sulfametopyrazine combination in a Plasmodium falciparum endemic zone].", "content": "The duration of action of the drug Antemal, a combination of Pyrimethamine and Sulfametopyrazine, was eveluated in Bobo-Dioulasso, Upper Volta, West Africa, and endemic zone for Plasmodium falciparum malaria. The study was held during the season of maximum malaria transmission. 79 persons presenting with an acute attack of malaria were studied; 37 persons received a single dose of chloroquine sulfate (Nivaquine), at a dose of 15 mg./kg.; 42 persons received a single dose of Antemal at a dose of one tablet (75 mg. of Pyrimethamine and 25 mg. of Sulfametopyrazine) per 10 kg. Clinical and parasitological studies of all subjects occurred on days one, two, three, 10, 17, 24 and 31. Only one of 37 (2.7%) subjects on Antemal had a reappearance of trophozoites, occurring on day 17. Eight of 42 (19.0%) patients taking nivaquine had reappearance of trophozoites, observed between day 23 and 31. Gametocytes were observed in eight of 37 (21.6 %) persons on Antemal and in only one of 42 (2.3 %) persons on Nivaquine. These observations suggest an extended duration of protection from Antemal in semi-immune individuals. Nivaquine appeared as a potent inhibitor of gametocytogenesis.", "contents": "[Duration of action of the pyrimethamine-sulfametopyrazine combination in a Plasmodium falciparum endemic zone]. The duration of action of the drug Antemal, a combination of Pyrimethamine and Sulfametopyrazine, was eveluated in Bobo-Dioulasso, Upper Volta, West Africa, and endemic zone for Plasmodium falciparum malaria. The study was held during the season of maximum malaria transmission. 79 persons presenting with an acute attack of malaria were studied; 37 persons received a single dose of chloroquine sulfate (Nivaquine), at a dose of 15 mg./kg.; 42 persons received a single dose of Antemal at a dose of one tablet (75 mg. of Pyrimethamine and 25 mg. of Sulfametopyrazine) per 10 kg. Clinical and parasitological studies of all subjects occurred on days one, two, three, 10, 17, 24 and 31. Only one of 37 (2.7%) subjects on Antemal had a reappearance of trophozoites, occurring on day 17. Eight of 42 (19.0%) patients taking nivaquine had reappearance of trophozoites, observed between day 23 and 31. Gametocytes were observed in eight of 37 (21.6 %) persons on Antemal and in only one of 42 (2.3 %) persons on Nivaquine. These observations suggest an extended duration of protection from Antemal in semi-immune individuals. Nivaquine appeared as a potent inhibitor of gametocytogenesis."}
{"id": "PMID:1100286", "title": "[What was the fate of patients with leprosy during the plague pandemia in the middle ages (1348-1350)].", "content": "The author thinks that, if lepra has suddenly decreased in Europe from the 14th century, it is because the most severe cases, i.e. the most contagious ones, disappeared during the hecatombs caused between 1348 and 1350 by the \"Black Death\", the black plague, which took most often the pulmonary form. The author disproves the opinion of those who think that lepers died from plague. He thinks that lepers' death was secondary to that of the monks who, at this time, cared for these outcases, and thanks to their self-sacrifice permitted these lepers' survival. The monks were more exposed to contagion; obliged by their vocation and by pope's command to help the dyings and to give them sacraments, they were obliged to leave lepers to their fate. Like domestic animals, the latter died of hunger probably, any corpse or carcass being considered as plague victims. Supporting this opinion, the author reports his observations at Madagascar, where no leper of the leper-houses of Madascar center, a plague focus still to-day but very active between 1922 and 1936, contracted plague. On the other hand, experiments with \"leprous\" rats (Stefansky bacillus) showed a significant resistance of these animals to an experimental plague infection.", "contents": "[What was the fate of patients with leprosy during the plague pandemia in the middle ages (1348-1350)]. The author thinks that, if lepra has suddenly decreased in Europe from the 14th century, it is because the most severe cases, i.e. the most contagious ones, disappeared during the hecatombs caused between 1348 and 1350 by the \"Black Death\", the black plague, which took most often the pulmonary form. The author disproves the opinion of those who think that lepers died from plague. He thinks that lepers' death was secondary to that of the monks who, at this time, cared for these outcases, and thanks to their self-sacrifice permitted these lepers' survival. The monks were more exposed to contagion; obliged by their vocation and by pope's command to help the dyings and to give them sacraments, they were obliged to leave lepers to their fate. Like domestic animals, the latter died of hunger probably, any corpse or carcass being considered as plague victims. Supporting this opinion, the author reports his observations at Madagascar, where no leper of the leper-houses of Madascar center, a plague focus still to-day but very active between 1922 and 1936, contracted plague. On the other hand, experiments with \"leprous\" rats (Stefansky bacillus) showed a significant resistance of these animals to an experimental plague infection."}
{"id": "PMID:1100294", "title": "A field with a liver measles-mumps-rubella vaccine.", "content": "Three lots of Lirutrin (measles-mumps-rubella live, attenuated virus vaccine) were evaluated in a double-blind, placebo-controlled clinical study involoving 273 children. The vaccine consisted of a combination of Schwarz strain measles, Jeryl Lynn strain mumps, and Cendehill strain rubella vaccine viruses. The frequency of positive clinical findings was essentially the same in susceptiable vaccinees, immune vaccinees, and placebo recipients; indicating that the observed symptoms were not caused by the vaccine. Antibody assay of paired serum samples revealed that measles, mumps, and rubella seroconverison rates in triple susceptible vaccinees were excellent, ranging from 96 to 98 per cent with all three lots combined. Results of this study show that Lirutrin trivalent vaccine is well tolerated and highly effective.", "contents": "A field with a liver measles-mumps-rubella vaccine. Three lots of Lirutrin (measles-mumps-rubella live, attenuated virus vaccine) were evaluated in a double-blind, placebo-controlled clinical study involoving 273 children. The vaccine consisted of a combination of Schwarz strain measles, Jeryl Lynn strain mumps, and Cendehill strain rubella vaccine viruses. The frequency of positive clinical findings was essentially the same in susceptiable vaccinees, immune vaccinees, and placebo recipients; indicating that the observed symptoms were not caused by the vaccine. Antibody assay of paired serum samples revealed that measles, mumps, and rubella seroconverison rates in triple susceptible vaccinees were excellent, ranging from 96 to 98 per cent with all three lots combined. Results of this study show that Lirutrin trivalent vaccine is well tolerated and highly effective."}
{"id": "PMID:1100295", "title": "Cephalexin in the management of bacteriuria.", "content": "This report evaluates the effectiveness of cephalexin in the treatment of bacteriuria in 93 children. Cephalexin therapy eradicated sensitive organisms in 97 per cent of the cases regardless of recurrence, structural abnormality, or status of renal function. Nevertheless, recurrences with a new, resistant organism occurred significantly in certain patients, expecially those with major anatomic abnormalities, during a six-week follow-up period. The incidence of drug reactions was low.", "contents": "Cephalexin in the management of bacteriuria. This report evaluates the effectiveness of cephalexin in the treatment of bacteriuria in 93 children. Cephalexin therapy eradicated sensitive organisms in 97 per cent of the cases regardless of recurrence, structural abnormality, or status of renal function. Nevertheless, recurrences with a new, resistant organism occurred significantly in certain patients, expecially those with major anatomic abnormalities, during a six-week follow-up period. The incidence of drug reactions was low."}
{"id": "PMID:1100298", "title": "Comparison of cardiac effects of timolol and propranolol.", "content": "The cardiac effects of the beta-adrenergic blocking agent, timolol, were compared to those of propranolol after intravenous administration in 6 healthy male subjects. The effects of timolol and propranolol on maximal exercise-induced tachycardia were measured. Timolol was about ten times as potent as propranolol in antagonizing exercise-induced tachycardia. Dose-response curves to intravenous infusions of isoproterenol were obtained after three doses of each beta-antagonist. Changes in myocardial contractility were assessed by cardiac systolic time intervals and were compared to the simultaneous changes in heart rate elicited by isoproterenol. For the 6 subjects, the mean potency ratios of timolol to propranolol as an antagonist of the chronotropic and inotropic effects of isoproterenol were 13.8 (+/-0.8) and 12.7 (+/-1.0), respectively. Neither timolol nor propranolol acted more selectively on beta-receptors affecting heart rate than on those influencing contractility.", "contents": "Comparison of cardiac effects of timolol and propranolol. The cardiac effects of the beta-adrenergic blocking agent, timolol, were compared to those of propranolol after intravenous administration in 6 healthy male subjects. The effects of timolol and propranolol on maximal exercise-induced tachycardia were measured. Timolol was about ten times as potent as propranolol in antagonizing exercise-induced tachycardia. Dose-response curves to intravenous infusions of isoproterenol were obtained after three doses of each beta-antagonist. Changes in myocardial contractility were assessed by cardiac systolic time intervals and were compared to the simultaneous changes in heart rate elicited by isoproterenol. For the 6 subjects, the mean potency ratios of timolol to propranolol as an antagonist of the chronotropic and inotropic effects of isoproterenol were 13.8 (+/-0.8) and 12.7 (+/-1.0), respectively. Neither timolol nor propranolol acted more selectively on beta-receptors affecting heart rate than on those influencing contractility."}
{"id": "PMID:1100299", "title": "Effects of marihuana combined with secobarbital.", "content": "Twelve male volunteers smoked a marihuana cigarette prepared to deliver 0 or 25 mug/kg tetrahydrocannabinol (THC) 50 min after ingesting a capsule containing either placebo or 150 mg/70 kg sodium secobarbital. Drugs were administered in a double-blind manner, and all treatments were assigned to each subject in a randomized complete block design. Objective and subjective tests designed to measure mental and motor performance indicated that marihuana impaired stability, hand-eye coordination, and mental performance. Secobarbital affected motor performance, manual coordination, and mental performance. In combination, marihuana and secobarbital had an additive effect on subjective responses and impairment in certain psychomotor performance tests.", "contents": "Effects of marihuana combined with secobarbital. Twelve male volunteers smoked a marihuana cigarette prepared to deliver 0 or 25 mug/kg tetrahydrocannabinol (THC) 50 min after ingesting a capsule containing either placebo or 150 mg/70 kg sodium secobarbital. Drugs were administered in a double-blind manner, and all treatments were assigned to each subject in a randomized complete block design. Objective and subjective tests designed to measure mental and motor performance indicated that marihuana impaired stability, hand-eye coordination, and mental performance. Secobarbital affected motor performance, manual coordination, and mental performance. In combination, marihuana and secobarbital had an additive effect on subjective responses and impairment in certain psychomotor performance tests."}
{"id": "PMID:1100300", "title": "Triazolam in insomniac family practice patients.", "content": "Triazolam, 0.5 mg, a benzodiazepine with hypnotic properties, was compared to secobarbital, 100 mg, and placebo in a 1-wk study conducted with 100 insomniac family practice patients. Considerable sensitivity to differential treatment effects was demonstrated for these family practice patients as well as for a research methodology that combines a crossover design, permitting preference ratings, with a between-patient design. In almost all sleep parameters, assessed with a variety of subjective techniques, triazolam and secobarbital were shown to be significantly more effective than placebo. Triazolam was consistently and often significantly indicated to be a more effective hypnotic, particularly for reducing nocturnal awakening, than secobarbital. Analysis of self-report emotional distress data revealed that present insomniac patients were slightly more emotionally symptomatic than other nonpsychiatric populations. Triazolam was followed by the greatest and secobarbital the least relief of emotional symptoms and triazolam emerged as an especially effective hypnotic for initially more depressed insomniac patients. Present findings suggest that type and degree of emotional symptomatology may affect the response of insomniac patients to hypnotics.", "contents": "Triazolam in insomniac family practice patients. Triazolam, 0.5 mg, a benzodiazepine with hypnotic properties, was compared to secobarbital, 100 mg, and placebo in a 1-wk study conducted with 100 insomniac family practice patients. Considerable sensitivity to differential treatment effects was demonstrated for these family practice patients as well as for a research methodology that combines a crossover design, permitting preference ratings, with a between-patient design. In almost all sleep parameters, assessed with a variety of subjective techniques, triazolam and secobarbital were shown to be significantly more effective than placebo. Triazolam was consistently and often significantly indicated to be a more effective hypnotic, particularly for reducing nocturnal awakening, than secobarbital. Analysis of self-report emotional distress data revealed that present insomniac patients were slightly more emotionally symptomatic than other nonpsychiatric populations. Triazolam was followed by the greatest and secobarbital the least relief of emotional symptoms and triazolam emerged as an especially effective hypnotic for initially more depressed insomniac patients. Present findings suggest that type and degree of emotional symptomatology may affect the response of insomniac patients to hypnotics."}
{"id": "PMID:1100301", "title": "Penfluridol steady-state kinetics in psychiatric patients.", "content": "Twenty-two hospitalized schizophrenic patients, participating in a large-scale phase II double-blind dose-effect study (30, 60, and 120 mg weekly) of penfluridol, a new diphenylbutylpiperidine neuroleptic, were maintained on a regular dosage regimen for 13 wk. Several blood samples were taken during the last dosage interval. Results show that the peak concentration develops within 12 hr after the last dose. A rapid decline, probably due to tissue re-equilibration, then occurs and is followed by a much slower falloff. Detectable concentrations 168 hr after administration are consistent with the long duration of action of penfluridol. Statistically significant differences between doses were found in the analysis of variance of plasma concentrations at all sampling times and in mean steady-state plasm concentrations. Wide differences in plasma concentrations were noted in patients receiving the same absolute dose, but a good relationship was defined between mean steady-state concentration and the dose expressed as mg per either kg of body weight or square meter of body surface area.", "contents": "Penfluridol steady-state kinetics in psychiatric patients. Twenty-two hospitalized schizophrenic patients, participating in a large-scale phase II double-blind dose-effect study (30, 60, and 120 mg weekly) of penfluridol, a new diphenylbutylpiperidine neuroleptic, were maintained on a regular dosage regimen for 13 wk. Several blood samples were taken during the last dosage interval. Results show that the peak concentration develops within 12 hr after the last dose. A rapid decline, probably due to tissue re-equilibration, then occurs and is followed by a much slower falloff. Detectable concentrations 168 hr after administration are consistent with the long duration of action of penfluridol. Statistically significant differences between doses were found in the analysis of variance of plasma concentrations at all sampling times and in mean steady-state plasm concentrations. Wide differences in plasma concentrations were noted in patients receiving the same absolute dose, but a good relationship was defined between mean steady-state concentration and the dose expressed as mg per either kg of body weight or square meter of body surface area."}
{"id": "PMID:1100302", "title": "Bioavailability of dexamethasone. II. Dexamethasone phosphate.", "content": "Plasma levels of dexamethasone phosphate (DP) and dexamethasone free alcohol (DA) were determined by a modification of existing radioimmunoassay methodology following intravenous administration of DP in man. Areas under DA plasma profiles were a linear function of DP dosage over the 40-fold range 0.05 to 2.0 mg/kg, and, by comparison with values obtained after DA was intravenously administered, indicated an overall conversion of DP to DA of 90%. The first-order rate constant for the conversion, 4.03 hr-1, was approximately 25 times that for hydrolysis in whole blood incubated in vitro. This relationship as well as disposition kinetics suggested that the major component of DP hydrolysis occurs within highly perfused organ(s) comprising the central kinetic compartment. Eighteen subjects were studied in a crossover experiment, and no significant differences were observed in best-fit parameters for the 4 mg/ml parenteral solution of DP in current use and an experimental high potency preparation of 24 mg/ml.", "contents": "Bioavailability of dexamethasone. II. Dexamethasone phosphate. Plasma levels of dexamethasone phosphate (DP) and dexamethasone free alcohol (DA) were determined by a modification of existing radioimmunoassay methodology following intravenous administration of DP in man. Areas under DA plasma profiles were a linear function of DP dosage over the 40-fold range 0.05 to 2.0 mg/kg, and, by comparison with values obtained after DA was intravenously administered, indicated an overall conversion of DP to DA of 90%. The first-order rate constant for the conversion, 4.03 hr-1, was approximately 25 times that for hydrolysis in whole blood incubated in vitro. This relationship as well as disposition kinetics suggested that the major component of DP hydrolysis occurs within highly perfused organ(s) comprising the central kinetic compartment. Eighteen subjects were studied in a crossover experiment, and no significant differences were observed in best-fit parameters for the 4 mg/ml parenteral solution of DP in current use and an experimental high potency preparation of 24 mg/ml."}
{"id": "PMID:1100303", "title": "Suppression of cortisol by a progestational steroid, melengestrol.", "content": "Melengestrol, a steroid with progestational activity but with a structure that does not resemble that of cortisol, suppresses plasma cortisol in humans with a potency 1/40th that of dexamethasone. The 6alpha-methyl and 17alpha-acetoxy substituents and the 6,7 double bond of melengestrol acetate are known to slow the metabolism of similar steroids. Insertion of a 6alpha-methyl group markedly increases the glucocorticoid activity of 17alpha-OH-progesterone. The glucocorticoid activity of melengestrol is presumably attributable to the large dose administered in our study, its slow metabolism and limitations of the specificity of glucocorticoid tissue receptors.", "contents": "Suppression of cortisol by a progestational steroid, melengestrol. Melengestrol, a steroid with progestational activity but with a structure that does not resemble that of cortisol, suppresses plasma cortisol in humans with a potency 1/40th that of dexamethasone. The 6alpha-methyl and 17alpha-acetoxy substituents and the 6,7 double bond of melengestrol acetate are known to slow the metabolism of similar steroids. Insertion of a 6alpha-methyl group markedly increases the glucocorticoid activity of 17alpha-OH-progesterone. The glucocorticoid activity of melengestrol is presumably attributable to the large dose administered in our study, its slow metabolism and limitations of the specificity of glucocorticoid tissue receptors."}
{"id": "PMID:1100304", "title": "Correlation of plateau serum salicylate level with rate of salicylate metabolism.", "content": "The range of plateau serum salicylate concentrations was 4.4 to 33 mg/100 ml in patients with rheumatoid arthritis after they were treated with 50 mg/kg of aspirin daily for 5 days. Individual plateau serum levels correlated better with urinary excretion rates of the metabolite, salicylurate (whose maximum production is capacity-limited), than with total urinary excretion of salicylates. These observations suggest that large intersubject variations in plateau serum salicylate levels are determined, at least in part, by similar differences in the maximum rates of capacity-limited metabolic reactions. For optimal therapeutic responses, individualization of aspirin dosage by following serum salicylate levels is recommended.", "contents": "Correlation of plateau serum salicylate level with rate of salicylate metabolism. The range of plateau serum salicylate concentrations was 4.4 to 33 mg/100 ml in patients with rheumatoid arthritis after they were treated with 50 mg/kg of aspirin daily for 5 days. Individual plateau serum levels correlated better with urinary excretion rates of the metabolite, salicylurate (whose maximum production is capacity-limited), than with total urinary excretion of salicylates. These observations suggest that large intersubject variations in plateau serum salicylate levels are determined, at least in part, by similar differences in the maximum rates of capacity-limited metabolic reactions. For optimal therapeutic responses, individualization of aspirin dosage by following serum salicylate levels is recommended."}
{"id": "PMID:1100305", "title": "Pharmacokinetics of indomethacin.", "content": "Plasma concentrations of indomethacin have been studied in 5 healthy volunteers after single and multiple doses (25 mg intravenously [iv], 25, 50, and 100 mg orally, 100 mg rectally, and 25 mg three times daily [tid]. In 8 other normal subjects and in 5 patients a 50-mg oral dose of indomethacin was given and the indomethacin concentration was followed from 8 to 32 hr after dosing. All analyses were carried out using a new mass fragmentographic method. After oral and rectal doses the plasma decay of indomethacin was biphasic, and the data were interpreted according to a 2-compartment open model. The half-life of the beta-phase varied between 2.6 and 11.2 hr. The volume of distribution ranged from 0.34 to 1.57 L/kg and the plasma clearance from 0.044 to 0.109 L/kg/hr. There was no evidence of dose-dependent elimination. Indomethacin was rapidly and well absorbed after oral dosing with peak plasma concentrations within 2 hr. Comparison with the area under the curve (AUC) after iv dosing indicated complete bioavailability. The AUC after rectal dosing indicated complete bioavailability, but the rate of absorption was slower than after oral administration. Indomethacin, 25 mg three times daily, was also given for 9 days to the same normal subjects; equilibrium concentrations obtained did not differ significantly (p greater than 0.1) from those predicted from single-dose data in the 5 subjects.", "contents": "Pharmacokinetics of indomethacin. Plasma concentrations of indomethacin have been studied in 5 healthy volunteers after single and multiple doses (25 mg intravenously [iv], 25, 50, and 100 mg orally, 100 mg rectally, and 25 mg three times daily [tid]. In 8 other normal subjects and in 5 patients a 50-mg oral dose of indomethacin was given and the indomethacin concentration was followed from 8 to 32 hr after dosing. All analyses were carried out using a new mass fragmentographic method. After oral and rectal doses the plasma decay of indomethacin was biphasic, and the data were interpreted according to a 2-compartment open model. The half-life of the beta-phase varied between 2.6 and 11.2 hr. The volume of distribution ranged from 0.34 to 1.57 L/kg and the plasma clearance from 0.044 to 0.109 L/kg/hr. There was no evidence of dose-dependent elimination. Indomethacin was rapidly and well absorbed after oral dosing with peak plasma concentrations within 2 hr. Comparison with the area under the curve (AUC) after iv dosing indicated complete bioavailability. The AUC after rectal dosing indicated complete bioavailability, but the rate of absorption was slower than after oral administration. Indomethacin, 25 mg three times daily, was also given for 9 days to the same normal subjects; equilibrium concentrations obtained did not differ significantly (p greater than 0.1) from those predicted from single-dose data in the 5 subjects."}
{"id": "PMID:1100306", "title": "Relative potency of prorenoate and spironolactone in normal man.", "content": "The potency of single doses of a new aldosterone antagonist, prorenoate, in reversing the renal effects of the synthetic mineralocorticoid fludrocortisone was compared to that of spironolactone in a double-blind balanced crossover study in 12 healthy subjects. The potency of prorenoate potassium as related to elevation of the urinary log 10 10 Na/K ratio (2.69:1) and as related to potassium retention (3.75:1) was significantly higher than that of spironolactone. Prorenoate produced greater natriuresis (1.64:1) but the difference was not significant. There was evidence for a qualitative difference between spironolactone and prorenoate; the latter significantly more potent in retaining potassium than in increasing sodium excretion. The simple methodology described is based on standard bioassay principles, yielded a valid and sensitive comparison of the two drugs, and should prove useful in the evaluation of other aldosterone antagonists.", "contents": "Relative potency of prorenoate and spironolactone in normal man. The potency of single doses of a new aldosterone antagonist, prorenoate, in reversing the renal effects of the synthetic mineralocorticoid fludrocortisone was compared to that of spironolactone in a double-blind balanced crossover study in 12 healthy subjects. The potency of prorenoate potassium as related to elevation of the urinary log 10 10 Na/K ratio (2.69:1) and as related to potassium retention (3.75:1) was significantly higher than that of spironolactone. Prorenoate produced greater natriuresis (1.64:1) but the difference was not significant. There was evidence for a qualitative difference between spironolactone and prorenoate; the latter significantly more potent in retaining potassium than in increasing sodium excretion. The simple methodology described is based on standard bioassay principles, yielded a valid and sensitive comparison of the two drugs, and should prove useful in the evaluation of other aldosterone antagonists."}
{"id": "PMID:1100307", "title": "Temporal variations in acetaminophen and phenacetin half-life in man.", "content": "Acetaminophen (APAP) and phenacetin mean plasma half-lives were approximately 15% longer in normal male volunteers at 6 A.M. than at 2 P.M. as determined after oral administration of each drug at 6 A.M. and 2 P.M. Concentrations of plasma 11-hydroxycorticoids (11-OHCS) in these volunteers were approximately 42% higher at 6 A.M. than at 2 P.M. The mean apparent volume of distribution (aVd) of APAP decreased by approximately 13% from 6 A.M. to 2 P.M., whereas the mean metabolic clearance rate (MCR) of APAP did not change significantly. Neither the mean aVd nor the mean MCR of phenacetin changed significantly from 6 A.M. to 2 P.M. Measurement of urinary metabolites showed no alteration in APAP glucuronidation in the 8-hr period following APAP administration at 6 A.M. or 2 P.M., but indicated that after phenacetin administration 10% more of the dose was oxidatively metabolized to APAP during a 24-hr period beginning at 2 P.M. than at 6 A.M. Within each subject, plasma acetaminophen or phenacetin half-life at 2 P.M. or 6 A.M. was highly reproducible, but large interindividual variations occurred in the extent of temporal variation during this period.", "contents": "Temporal variations in acetaminophen and phenacetin half-life in man. Acetaminophen (APAP) and phenacetin mean plasma half-lives were approximately 15% longer in normal male volunteers at 6 A.M. than at 2 P.M. as determined after oral administration of each drug at 6 A.M. and 2 P.M. Concentrations of plasma 11-hydroxycorticoids (11-OHCS) in these volunteers were approximately 42% higher at 6 A.M. than at 2 P.M. The mean apparent volume of distribution (aVd) of APAP decreased by approximately 13% from 6 A.M. to 2 P.M., whereas the mean metabolic clearance rate (MCR) of APAP did not change significantly. Neither the mean aVd nor the mean MCR of phenacetin changed significantly from 6 A.M. to 2 P.M. Measurement of urinary metabolites showed no alteration in APAP glucuronidation in the 8-hr period following APAP administration at 6 A.M. or 2 P.M., but indicated that after phenacetin administration 10% more of the dose was oxidatively metabolized to APAP during a 24-hr period beginning at 2 P.M. than at 6 A.M. Within each subject, plasma acetaminophen or phenacetin half-life at 2 P.M. or 6 A.M. was highly reproducible, but large interindividual variations occurred in the extent of temporal variation during this period."}
{"id": "PMID:1100308", "title": "Bioavailability and single-dose pharmacokinetics of intramuscular phenytoin.", "content": "Comparison of phenytoin plasma concentrations in an intravenous and intramuscular crossover study in 12 healthy ambulant, male subjects indicates that phenytoin administered intramuscularly is absorbed over a period of approximately five days. A model simulating precipitation and redissolution of the drug at the injection site has been shown to satisfactorily fit observed plasma concentration data following intramuscular administration. It is proposed that this model will be useful in the selection of an appropriate dosing regimen in situations in which intramuscular administration of phenytoin is indicated.", "contents": "Bioavailability and single-dose pharmacokinetics of intramuscular phenytoin. Comparison of phenytoin plasma concentrations in an intravenous and intramuscular crossover study in 12 healthy ambulant, male subjects indicates that phenytoin administered intramuscularly is absorbed over a period of approximately five days. A model simulating precipitation and redissolution of the drug at the injection site has been shown to satisfactorily fit observed plasma concentration data following intramuscular administration. It is proposed that this model will be useful in the selection of an appropriate dosing regimen in situations in which intramuscular administration of phenytoin is indicated."}
{"id": "PMID:1100309", "title": "Combination 6-mercaptopurine-adriamycin in refractory adult acute leukemia.", "content": "Remission induction therapy with 6MP and adriamycin in combination was administered to 19 adult leukemic patients refractory to previous therapy. Eight patients also received vincristine and prednisone. Thirteen patients had acute myelogenous leukemia, 3 undifferentiated leukemia, and 3 blastic transformation of chronic myelogenous leukemia. Four patients achieved remission but in only 2 were the remissions complete. Eleven patients failed to respond. Ten of the 19 patients developed unexpected severe liver toxicity manifested by a clinical picture of cholestasis (in the majority) or ascending cholangitis (in 2 patients). In the postmortem examination of 8 patients there was cholestasis and mild to severe hepatocellular damage in all.", "contents": "Combination 6-mercaptopurine-adriamycin in refractory adult acute leukemia. Remission induction therapy with 6MP and adriamycin in combination was administered to 19 adult leukemic patients refractory to previous therapy. Eight patients also received vincristine and prednisone. Thirteen patients had acute myelogenous leukemia, 3 undifferentiated leukemia, and 3 blastic transformation of chronic myelogenous leukemia. Four patients achieved remission but in only 2 were the remissions complete. Eleven patients failed to respond. Ten of the 19 patients developed unexpected severe liver toxicity manifested by a clinical picture of cholestasis (in the majority) or ascending cholangitis (in 2 patients). In the postmortem examination of 8 patients there was cholestasis and mild to severe hepatocellular damage in all."}
{"id": "PMID:1100310", "title": "Effects of ticlopidine, a new platelet aggregation inhibitor in man.", "content": "Ticlopidine is a new platelet aggregation inhibitor. The effect of this drug was studied on 55 subjects, healthy volunteers and hospitalized patients. The action requires 24 to 48 hr to appear, and lasts more than 3 days. A dose-effect relationship was studied with oral daily doses ranging from 250 to 1,000 mg during 1 wk; it showed a 50% inhibition on adenosine diphosphate (ADP)-induced aggregation at 2 muM concentration on an oral daily dose of 450 mg. No action was found on collagen-induced aggregation, and a mild effect was observed on platelet adhesiveness. Clinical tolerance was assessed in patients given ticlopidine in oral doses up to 500 mg/day during several weeks, showing no overt side effects and no change in the safety parameters.", "contents": "Effects of ticlopidine, a new platelet aggregation inhibitor in man. Ticlopidine is a new platelet aggregation inhibitor. The effect of this drug was studied on 55 subjects, healthy volunteers and hospitalized patients. The action requires 24 to 48 hr to appear, and lasts more than 3 days. A dose-effect relationship was studied with oral daily doses ranging from 250 to 1,000 mg during 1 wk; it showed a 50% inhibition on adenosine diphosphate (ADP)-induced aggregation at 2 muM concentration on an oral daily dose of 450 mg. No action was found on collagen-induced aggregation, and a mild effect was observed on platelet adhesiveness. Clinical tolerance was assessed in patients given ticlopidine in oral doses up to 500 mg/day during several weeks, showing no overt side effects and no change in the safety parameters."}
{"id": "PMID:1100323", "title": "Galactose-specific messenger ribonucleic acid contents in Escherichia coli: effect of inducer, gene dosage and galactose genotype.", "content": "Galactose specific mRNA (gal-mRNA) and galactokinase were measured in strains of E.coli with varying numbers of copies of the galactose operon. While the fucose induction the amount of gal mRNA has been found to be proportional to the content of galactokinase and to the gene frequency, with galactose induction this was not the case. It is suggested that this is a result of the metabolism of galactose leading to catabolite repression. The amounts of gal-mRNA and galactokinase were also measured in a series of polar mutants. With increasing polarity, there was a greater effect on enzyme content than on gal-mRNA. This suggests that the effect of polarity on RNA is the result of degradation after synthesis rather than prevention of transcription. A method of correlating hybridisation data with the genetic map is described.", "contents": "Galactose-specific messenger ribonucleic acid contents in Escherichia coli: effect of inducer, gene dosage and galactose genotype. Galactose specific mRNA (gal-mRNA) and galactokinase were measured in strains of E.coli with varying numbers of copies of the galactose operon. While the fucose induction the amount of gal mRNA has been found to be proportional to the content of galactokinase and to the gene frequency, with galactose induction this was not the case. It is suggested that this is a result of the metabolism of galactose leading to catabolite repression. The amounts of gal-mRNA and galactokinase were also measured in a series of polar mutants. With increasing polarity, there was a greater effect on enzyme content than on gal-mRNA. This suggests that the effect of polarity on RNA is the result of degradation after synthesis rather than prevention of transcription. A method of correlating hybridisation data with the genetic map is described."}
{"id": "PMID:1100325", "title": "Idiopathic pulmonary hemosiderosis. Electron microscopic, immunofluorescent, and iron kinetic studies.", "content": "The clinical course of a 37-year-old white man with idiopathic pulmonary hemosiderosis is presented. This patient is unusual in that he has had repeated exacerbations and remissions over a period of seven years and remains currently in spontaneous remission with no therapy. Routine sections of the lung biopsy revealed characteristec findings. Immunofluorescence staining of the lung was negative, and electron microscopic studies showed only nonspecific findings. While in remission, 51chromium-labelled red-blood-cell survival studies and 59iron kinetic studies were performed; the results were normal.", "contents": "Idiopathic pulmonary hemosiderosis. Electron microscopic, immunofluorescent, and iron kinetic studies. The clinical course of a 37-year-old white man with idiopathic pulmonary hemosiderosis is presented. This patient is unusual in that he has had repeated exacerbations and remissions over a period of seven years and remains currently in spontaneous remission with no therapy. Routine sections of the lung biopsy revealed characteristec findings. Immunofluorescence staining of the lung was negative, and electron microscopic studies showed only nonspecific findings. While in remission, 51chromium-labelled red-blood-cell survival studies and 59iron kinetic studies were performed; the results were normal."}
{"id": "PMID:1100337", "title": "[Fungal infections after renal transplantation (author's transl)].", "content": "21 of 41 patients developed clinically manifest or systemic Candida albicans infection 1-36 months after renal transplantation. Asymptomatic candiduria was diagnosed in all patients even before the onset of clinical symptoms. Fungal stomatitis was the most frequent clinical sign, followed by mycotic changes in the respiratory, genito-urinary (vaginitis) and gastro-intestinal tract. In five cases intrahepatic biliary stasis was diagnosed in the course of a Candida albicans septicaemia. In 12 patients with renal transplants it was possible, by treatment with nystatin, clotrimazole, flucytosine, miconazole and amphotericine B to control a generalized or clinically manifest Candida albicans infection. Three died of the septicaemia or meningoencephalitis, six as the result of bacterial superinfections. Inspection of the mouth is an important means of early diagnosing fungal infections. Antimycotic treatment should be started if fungal cultures from urine are repeatedly positive even if the clinical findings are still negative.", "contents": "[Fungal infections after renal transplantation (author's transl)]. 21 of 41 patients developed clinically manifest or systemic Candida albicans infection 1-36 months after renal transplantation. Asymptomatic candiduria was diagnosed in all patients even before the onset of clinical symptoms. Fungal stomatitis was the most frequent clinical sign, followed by mycotic changes in the respiratory, genito-urinary (vaginitis) and gastro-intestinal tract. In five cases intrahepatic biliary stasis was diagnosed in the course of a Candida albicans septicaemia. In 12 patients with renal transplants it was possible, by treatment with nystatin, clotrimazole, flucytosine, miconazole and amphotericine B to control a generalized or clinically manifest Candida albicans infection. Three died of the septicaemia or meningoencephalitis, six as the result of bacterial superinfections. Inspection of the mouth is an important means of early diagnosing fungal infections. Antimycotic treatment should be started if fungal cultures from urine are repeatedly positive even if the clinical findings are still negative."}
{"id": "PMID:1100338", "title": "[Indirect immunofluorescence in the serological diagnosis of chronic pyelonephritis (comparison with indirect haemagglutination and the Widal reaction (author's transl)].", "content": "Using indirect immunofluorescence, o-antibodies against the homologous causative organism were determined in 119 adults with chronic pyelonephritis, during a one to two-and-a-half year period. Humoral antibodies were determined in the same group of patients with the Widal reaction and indirect haemagglutination. The results were compared with those in a group of adults without renal disease, using the same serological reactions. In adults with chronic pyelonephritis the antibody titre (by immunofluorescence) was above normal limits (as obtained in the healthy group) in 63.1%. The corresponding results for the Widal reaction and indirect haemagglutination reaction were 16.5% and 12, respectively. Indirect immunofluorescence is thus clearly better than the other two serological methods in the diagnosis of chronic pyelonephritis.", "contents": "[Indirect immunofluorescence in the serological diagnosis of chronic pyelonephritis (comparison with indirect haemagglutination and the Widal reaction (author's transl)]. Using indirect immunofluorescence, o-antibodies against the homologous causative organism were determined in 119 adults with chronic pyelonephritis, during a one to two-and-a-half year period. Humoral antibodies were determined in the same group of patients with the Widal reaction and indirect haemagglutination. The results were compared with those in a group of adults without renal disease, using the same serological reactions. In adults with chronic pyelonephritis the antibody titre (by immunofluorescence) was above normal limits (as obtained in the healthy group) in 63.1%. The corresponding results for the Widal reaction and indirect haemagglutination reaction were 16.5% and 12, respectively. Indirect immunofluorescence is thus clearly better than the other two serological methods in the diagnosis of chronic pyelonephritis."}
{"id": "PMID:1100346", "title": "[Effects of bridge parts on the gingiva].", "content": "Over the past few years, preparation and insertion of a dental bridges have been considerably modified and rationalized due to new methods and techniques. The former requirements the shape of pontics hat to meet are no longer fully valid. The behavior of the various materials (metals, resins, ceramics) in the mouth and especially towards the gingiva has been tested sufficiently. Knowledge regarding the functional conditions (flushing of the gingiva underneath bridges, turgor, abutment mobility, pulsation) has considerably increased in the past few years. Therefore recommendations regarding the shaping of pontics can be formulated that meet the anatomic, physiologic, and hygienic conditions as well as the esthetic wishes of the patients.", "contents": "[Effects of bridge parts on the gingiva]. Over the past few years, preparation and insertion of a dental bridges have been considerably modified and rationalized due to new methods and techniques. The former requirements the shape of pontics hat to meet are no longer fully valid. The behavior of the various materials (metals, resins, ceramics) in the mouth and especially towards the gingiva has been tested sufficiently. Knowledge regarding the functional conditions (flushing of the gingiva underneath bridges, turgor, abutment mobility, pulsation) has considerably increased in the past few years. Therefore recommendations regarding the shaping of pontics can be formulated that meet the anatomic, physiologic, and hygienic conditions as well as the esthetic wishes of the patients."}
{"id": "PMID:1100347", "title": "[Clinical study on the contact between the partial denture and the alveolar process].", "content": "53 clinically prepared bridges were examined with regard to the resting position of the bridge body on the alveolar ridge, before they were finally cemented. It was to be tested whether the ideal requirements of punctate or linear pressure-less resting was met and if not, how big the portion of the base resting on the gingiva was as compared to the total base. All bridge bodies were resting on the alveolar ridge without exerting pressure but with a more or less broad base. In 42 out of the 66 examined cases, the bridge body was in a 50 to 100% contact with the mucosa.", "contents": "[Clinical study on the contact between the partial denture and the alveolar process]. 53 clinically prepared bridges were examined with regard to the resting position of the bridge body on the alveolar ridge, before they were finally cemented. It was to be tested whether the ideal requirements of punctate or linear pressure-less resting was met and if not, how big the portion of the base resting on the gingiva was as compared to the total base. All bridge bodies were resting on the alveolar ridge without exerting pressure but with a more or less broad base. In 42 out of the 66 examined cases, the bridge body was in a 50 to 100% contact with the mucosa."}
{"id": "PMID:1100345", "title": "Antibacterial drugs today. I.", "content": "Since the development of the sulphonamides in the 1930s and the subsequent development of antibiotics from the 1940s onwards, there have now been many drugs developed which are capable of chemotherapeutic activity in a patient infected by a susceptible micro-organism. This review is concerned with precise descriptions of important groups of antimicrobial drugs, with emphasis being placed on the more recently developed drugs. With each group of drugs the pharmacology, major therapeutic indications, dosages and adverse reactions are discussed. Part I of the article discusses the sulphonamides, the natural and semi-synthetic penicillins, cotrimoxazole, chloramphenicol, tetracyclines, the macrolides, lincomycin and clindamycin, fusidic acid, and the urinary antiseptics. The place of each in therapy is defined.", "contents": "Antibacterial drugs today. I. Since the development of the sulphonamides in the 1930s and the subsequent development of antibiotics from the 1940s onwards, there have now been many drugs developed which are capable of chemotherapeutic activity in a patient infected by a susceptible micro-organism. This review is concerned with precise descriptions of important groups of antimicrobial drugs, with emphasis being placed on the more recently developed drugs. With each group of drugs the pharmacology, major therapeutic indications, dosages and adverse reactions are discussed. Part I of the article discusses the sulphonamides, the natural and semi-synthetic penicillins, cotrimoxazole, chloramphenicol, tetracyclines, the macrolides, lincomycin and clindamycin, fusidic acid, and the urinary antiseptics. The place of each in therapy is defined."}
{"id": "PMID:1100348", "title": "[Chemical analysis of methacrylate plastics].", "content": "It was the object of this study to find quick but exact methods for determining the components of quickly setting methacrylate materials for dentures, dental fillings (PMMA) and for bone surgery. Furthermore, lower-molecular components still being present in the hardened resin were tested. By measuring refraction index and by infrared spectrum and gas-chromatographical analyses the composition of the liquid is obtained. Dimethyl-p-toluidine, hydrochinon and cross-linking agents were determined spectrophotometrically. Filling agents, pigments, plasticizers and soluble components of the powder were determined gravimetrically. The presence of polystyrol is established by infrared analysis, that of benzoyl peroxide by spectroscopic analysis. As to the hardened material, the molecular weight is determined by viscosity measurements and the molecular weight distribution by gel permeability measurements. Residual monomer is found by gas chromatography.", "contents": "[Chemical analysis of methacrylate plastics]. It was the object of this study to find quick but exact methods for determining the components of quickly setting methacrylate materials for dentures, dental fillings (PMMA) and for bone surgery. Furthermore, lower-molecular components still being present in the hardened resin were tested. By measuring refraction index and by infrared spectrum and gas-chromatographical analyses the composition of the liquid is obtained. Dimethyl-p-toluidine, hydrochinon and cross-linking agents were determined spectrophotometrically. Filling agents, pigments, plasticizers and soluble components of the powder were determined gravimetrically. The presence of polystyrol is established by infrared analysis, that of benzoyl peroxide by spectroscopic analysis. As to the hardened material, the molecular weight is determined by viscosity measurements and the molecular weight distribution by gel permeability measurements. Residual monomer is found by gas chromatography."}
{"id": "PMID:1100349", "title": "[Findings on the functional planning of dentures].", "content": "Analysis of over 20,000 prosthetic treatments carried out in the area of one association of dentists working under the compulsory public health insurance plan shows that prior to the conclusion of the new financial agreements on prosthetics, a treatment plan based on the findings and aiming at appropriate functional rehabilitation had been prepared in half of all the cases only.", "contents": "[Findings on the functional planning of dentures]. Analysis of over 20,000 prosthetic treatments carried out in the area of one association of dentists working under the compulsory public health insurance plan shows that prior to the conclusion of the new financial agreements on prosthetics, a treatment plan based on the findings and aiming at appropriate functional rehabilitation had been prepared in half of all the cases only."}
{"id": "PMID:1100350", "title": "[Follow-up studies on model cast prostheses after 4 years wear].", "content": "Over a period of 4-1/2 years, patients having model cast dentures that had been inserted in the dental office were checked and submitted to follow-ups. It became evident that the socalled pre-prosthetical measures had been performed in a non-satisfying manner. In 69% of the patients, teeth had been extracted directly before inserting the model cast denture. As a result thereof, a majority of the dentures was sagging after a relatively short time and 70% of the dentures revealed non-occlusion in the final follow-up. 47% of the dentures had to be modified because of necessary tooth extractions, thereby reducing the original number of abutment teeth by 19%. About a third of the inserted model cast dentures showed gross deficiences, the percentage of socalled \"collar\" constructions was relatively high.", "contents": "[Follow-up studies on model cast prostheses after 4 years wear]. Over a period of 4-1/2 years, patients having model cast dentures that had been inserted in the dental office were checked and submitted to follow-ups. It became evident that the socalled pre-prosthetical measures had been performed in a non-satisfying manner. In 69% of the patients, teeth had been extracted directly before inserting the model cast denture. As a result thereof, a majority of the dentures was sagging after a relatively short time and 70% of the dentures revealed non-occlusion in the final follow-up. 47% of the dentures had to be modified because of necessary tooth extractions, thereby reducing the original number of abutment teeth by 19%. About a third of the inserted model cast dentures showed gross deficiences, the percentage of socalled \"collar\" constructions was relatively high."}
{"id": "PMID:1100351", "title": "[A data storage system in computer dialog for dental prosthetics].", "content": "It is reported on a data processing system with computer dialogue for the \"standardized sheet of findings\". The computer-readable form, improved error control, and optimum evaluation possibilities are emphasized as special advantages. Simple handling and optimum data protection permit to have this system operated by trained laymen.", "contents": "[A data storage system in computer dialog for dental prosthetics]. It is reported on a data processing system with computer dialogue for the \"standardized sheet of findings\". The computer-readable form, improved error control, and optimum evaluation possibilities are emphasized as special advantages. Simple handling and optimum data protection permit to have this system operated by trained laymen."}
{"id": "PMID:1100352", "title": "[Shape of the denture base and self-cleaning].", "content": "It is reported on the relationship between the extent of the gap between teeth and denture and the formation of plaque.", "contents": "[Shape of the denture base and self-cleaning]. It is reported on the relationship between the extent of the gap between teeth and denture and the formation of plaque."}
{"id": "PMID:1100353", "title": "[Measurement of the pressure in dental casting].", "content": "The tests showed that the pressure exerted on the tooth stump and the surrounding tissue is determined by the consistency of the impression material, the amount escaping from the impression tray, the adaptation pressure and adaptation speed. The original consistency of the impression material without exposure to a catalyst is an especially important factor. If the material has a flow limit (e.g. Optosil with or without catalyst) it is exerting pressure in any case and this pressure continues even after setting. Impression materials without flow limit (e.g. Silaplast without catalyst) produce practically no pressure. Only by the activity of the catalyst a flow limit develops and thus pressure is produced.", "contents": "[Measurement of the pressure in dental casting]. The tests showed that the pressure exerted on the tooth stump and the surrounding tissue is determined by the consistency of the impression material, the amount escaping from the impression tray, the adaptation pressure and adaptation speed. The original consistency of the impression material without exposure to a catalyst is an especially important factor. If the material has a flow limit (e.g. Optosil with or without catalyst) it is exerting pressure in any case and this pressure continues even after setting. Impression materials without flow limit (e.g. Silaplast without catalyst) produce practically no pressure. Only by the activity of the catalyst a flow limit develops and thus pressure is produced."}
{"id": "PMID:1100354", "title": "[Development of an internal cooling mechanism for surgical drills].", "content": "An interior liquid cooling method was developed for surgical burs and tested in animal experiments as well as during the treatment of patients. The method ensures excellent cooling of the bone during surgical treatment.", "contents": "[Development of an internal cooling mechanism for surgical drills]. An interior liquid cooling method was developed for surgical burs and tested in animal experiments as well as during the treatment of patients. The method ensures excellent cooling of the bone during surgical treatment."}
{"id": "PMID:1100355", "title": "[Immediate prosthetic management of surgically treated maxillary lesions].", "content": "In accordance with a method previously published - in which undercut areas in the region of resection are utilized for retaining the denture - a modified method is presented permitting immediate prosthetical care for operated maxillary defects despite fresh wound surfaces and constrictio maxillarum.", "contents": "[Immediate prosthetic management of surgically treated maxillary lesions]. In accordance with a method previously published - in which undercut areas in the region of resection are utilized for retaining the denture - a modified method is presented permitting immediate prosthetical care for operated maxillary defects despite fresh wound surfaces and constrictio maxillarum."}
{"id": "PMID:1100356", "title": "[Jaw metastasis of sympathicoblastoma].", "content": "Using the example of a sympathicoblastoma metastasizing into the jaw, the symptomatology of this rare location of metastases is outlined. Jaw metastases of sympathicoblastomas occur in greater numbers only in children in the age of 2.5 to 5 years. Osteolytic jaw defects and unexplainable loosening of permanent molars in children should give rise to detailed examination with regard to the possible existence of a metastasizing malignant tumor (especially neuroblastoma). Despite a solitary metastasis, early diagnosis might have a decisive influence on the otherwise lethal course of the disease.", "contents": "[Jaw metastasis of sympathicoblastoma]. Using the example of a sympathicoblastoma metastasizing into the jaw, the symptomatology of this rare location of metastases is outlined. Jaw metastases of sympathicoblastomas occur in greater numbers only in children in the age of 2.5 to 5 years. Osteolytic jaw defects and unexplainable loosening of permanent molars in children should give rise to detailed examination with regard to the possible existence of a metastasizing malignant tumor (especially neuroblastoma). Despite a solitary metastasis, early diagnosis might have a decisive influence on the otherwise lethal course of the disease."}
{"id": "PMID:1100358", "title": "[The decalcification of teeth for histological studies by means of ultrasonics].", "content": "In order to accelerate decalcification of hard tissues for histological examinations, the additional application of ultrasonics is sometimes recommended. Comparative studies show that decalcification is not acclerated by ultrasonics.", "contents": "[The decalcification of teeth for histological studies by means of ultrasonics]. In order to accelerate decalcification of hard tissues for histological examinations, the additional application of ultrasonics is sometimes recommended. Comparative studies show that decalcification is not acclerated by ultrasonics."}
{"id": "PMID:1100359", "title": "[Clinical studies on a sodium monofluorophosphate toothpaste in school children].", "content": "Random tests made in school children of the Ludwigsburg district showed that after 3 years of uncontrolled use, the number of new DMF surfaces is smaller in children using Na2PO3F toothpaste and regularly cleaning their teeth than in the corresponding placebo groups. The caries-inhibiting effect was statistically significant (significance level p=0.05%). The increase in caries impact in school children regularly but insufficiently cleaning their teeth was also significant in the placebo groups.", "contents": "[Clinical studies on a sodium monofluorophosphate toothpaste in school children]. Random tests made in school children of the Ludwigsburg district showed that after 3 years of uncontrolled use, the number of new DMF surfaces is smaller in children using Na2PO3F toothpaste and regularly cleaning their teeth than in the corresponding placebo groups. The caries-inhibiting effect was statistically significant (significance level p=0.05%). The increase in caries impact in school children regularly but insufficiently cleaning their teeth was also significant in the placebo groups."}
{"id": "PMID:1100360", "title": "[Quantitative determination of the gingival sulcus fluid by means of microcapillaries].", "content": "Evaluation of the quantity and quality of crevicular fluid has become increasingly important in the past few years. Amount and composition of the gingival exudate reflects the physical condition of the gingival margin. For drawing off the gingival fluid primarily filter paper stripes are recommended. Some authors use microcapillaries made of glass. For facilitating measurements on the fluid a special method is described using standardized glass pipettes for collecting the gingival exudate.", "contents": "[Quantitative determination of the gingival sulcus fluid by means of microcapillaries]. Evaluation of the quantity and quality of crevicular fluid has become increasingly important in the past few years. Amount and composition of the gingival exudate reflects the physical condition of the gingival margin. For drawing off the gingival fluid primarily filter paper stripes are recommended. Some authors use microcapillaries made of glass. For facilitating measurements on the fluid a special method is described using standardized glass pipettes for collecting the gingival exudate."}
{"id": "PMID:1100361", "title": "[Statistical analysis of the activity of loxapine and thioridazine in a double-blind study].", "content": "49 patients have been treated for 12 weeks with loxapine and thioridazine in a double-blind study. The evaluation was carried out with a covariance analysis with B.P.R.S. and NOSIE as bases and a variance analysis concerning the general clinical improvement. With 36 schizophrenics, the two treatments have shown significant and similar general improvement as early as the second week, and this in particular with all the factors and most of the items of the B.P.R.S. with 13 psychotic not schizophrenic patients, the covariance analysis showed that the results (improvement of all the factors, with most of the items and in the total score of the B.P.R.S.), while comparable, were slightly in favour of loxapine for the item \"disorientation\", for \"disturbance of thought\", and \"excitement-disorientation\", for the final score of B.P.R.S., for the general and clinical improvement and for the alteration of the initial degree of severity. The analysis of the NOSIE showed a significant improvement which is similar for both treatments.", "contents": "[Statistical analysis of the activity of loxapine and thioridazine in a double-blind study]. 49 patients have been treated for 12 weeks with loxapine and thioridazine in a double-blind study. The evaluation was carried out with a covariance analysis with B.P.R.S. and NOSIE as bases and a variance analysis concerning the general clinical improvement. With 36 schizophrenics, the two treatments have shown significant and similar general improvement as early as the second week, and this in particular with all the factors and most of the items of the B.P.R.S. with 13 psychotic not schizophrenic patients, the covariance analysis showed that the results (improvement of all the factors, with most of the items and in the total score of the B.P.R.S.), while comparable, were slightly in favour of loxapine for the item \"disorientation\", for \"disturbance of thought\", and \"excitement-disorientation\", for the final score of B.P.R.S., for the general and clinical improvement and for the alteration of the initial degree of severity. The analysis of the NOSIE showed a significant improvement which is similar for both treatments."}
{"id": "PMID:1100362", "title": "Age-related changes in pituitary responsiveness to LHRH in the female rat.", "content": "Serum LH changes in response to LH-releasing hormone (LHRH) injection were measured in young (4-6 month-old) proestrous, estrous, and second-day-diestrous rats and in aged(23-30-month-old) constant estrous and irregular pseudopregnant (prolonged diestrus) female Long-Evans rats. Serum LH was measured by radioimmunoassay in serial blood samples taken before and at 15, 30 and 60 min after injection of 0, 5, 50 or 500 ng of LHRH. Serum LH also was assayed in similar groups of rats acutely pretreated with either 20 mug of estradiol benzoate (EB) or 5 mg of progesterone. Aged rats showed a smaller increase in serum LH following LHRH injection than the young groups. Within the young groups, the magnitude of response was greater in proestrous and estrous rats than in the diestrous animals. Pretreatment with EB resulted in increased serum LH after LHRH injection in young estrous and diestrous rats and in aged constant estrous rats, but did not alter serum LH levels in the aged pseudopregnant rats. Although the inccrease in serum LH after LHRH injection was greater in all young groups given progesterone pretreatment, progesterone pretreatment did not affect LHRH responsiveness in either of the aged groups. These results suggest that with aging, the pituitary becomes less capable of relasing LH in response to acute LHRH stimulation, and that the pituitaries of aged rats are less responsive to gonadal steroid sensitization to LHRH stimulation.", "contents": "Age-related changes in pituitary responsiveness to LHRH in the female rat. Serum LH changes in response to LH-releasing hormone (LHRH) injection were measured in young (4-6 month-old) proestrous, estrous, and second-day-diestrous rats and in aged(23-30-month-old) constant estrous and irregular pseudopregnant (prolonged diestrus) female Long-Evans rats. Serum LH was measured by radioimmunoassay in serial blood samples taken before and at 15, 30 and 60 min after injection of 0, 5, 50 or 500 ng of LHRH. Serum LH also was assayed in similar groups of rats acutely pretreated with either 20 mug of estradiol benzoate (EB) or 5 mg of progesterone. Aged rats showed a smaller increase in serum LH following LHRH injection than the young groups. Within the young groups, the magnitude of response was greater in proestrous and estrous rats than in the diestrous animals. Pretreatment with EB resulted in increased serum LH after LHRH injection in young estrous and diestrous rats and in aged constant estrous rats, but did not alter serum LH levels in the aged pseudopregnant rats. Although the inccrease in serum LH after LHRH injection was greater in all young groups given progesterone pretreatment, progesterone pretreatment did not affect LHRH responsiveness in either of the aged groups. These results suggest that with aging, the pituitary becomes less capable of relasing LH in response to acute LHRH stimulation, and that the pituitaries of aged rats are less responsive to gonadal steroid sensitization to LHRH stimulation."}
{"id": "PMID:1100363", "title": "Immunoreactive gonadotropin-releasing hormone (Gn-RH) during maturation in the rat: Ontogeny of regional hypothalamic differences.", "content": "The developmental appearance of immunoreactive gonandotropin releasing hormone (Gn-RH) was measured by radioimmunoassay in male and female rats from the 12th day of gestation to adulthood. Gn-RH content of the whole hypothalamus of both sexes increased from birth to 21 days of age. By the 28th day, Gn-RH content in females had approximately reached adult levels, while that in males continued to increase. Adult male rats exhibited significantly higher levels than adult females. The ontogeny of the regional differences previously observed in cycling female rats (4) was also studied. Regional analysis of Gn-RH development was characterized in the anterior hypothalamus by the rapid attainment of adult levels by day 7. In striking contrast, in the mid-hypothalamic region, Gn-RH content increased gradually, rising dramatically just prior to vaginal opening. The developmental pattern of Gn-RH content is consistent with the maturation of those hypothalamic structures concerned with synthesis, transport and storage of Gn-RH. The regional patterns observed may thus represent sequential maturation of the neural pathways involved.", "contents": "Immunoreactive gonadotropin-releasing hormone (Gn-RH) during maturation in the rat: Ontogeny of regional hypothalamic differences. The developmental appearance of immunoreactive gonandotropin releasing hormone (Gn-RH) was measured by radioimmunoassay in male and female rats from the 12th day of gestation to adulthood. Gn-RH content of the whole hypothalamus of both sexes increased from birth to 21 days of age. By the 28th day, Gn-RH content in females had approximately reached adult levels, while that in males continued to increase. Adult male rats exhibited significantly higher levels than adult females. The ontogeny of the regional differences previously observed in cycling female rats (4) was also studied. Regional analysis of Gn-RH development was characterized in the anterior hypothalamus by the rapid attainment of adult levels by day 7. In striking contrast, in the mid-hypothalamic region, Gn-RH content increased gradually, rising dramatically just prior to vaginal opening. The developmental pattern of Gn-RH content is consistent with the maturation of those hypothalamic structures concerned with synthesis, transport and storage of Gn-RH. The regional patterns observed may thus represent sequential maturation of the neural pathways involved."}
{"id": "PMID:1100364", "title": "Radioimmunoassay for luteinizing hormone releasing hormone in plasma.", "content": "A sensitive and specific double antibody radioimmunoassay has been developed capable of measuring LH-RH in extracted human plasma. Thyrotropin releasing hormone, lysine vasopressin and most of LH-RH analogues did not appear to affect the assay. Hypothalamic extract and some of the LH-RH analogues produced displacement curves which were parallel to that obtained with the synthetic LH-RH. Sensitivity of the radioimmunoassay was about 3 pg per assay tube. The coefficient of variation of intraassay was 6.4%, while that of interassay was 9.6%. Exogenous LH-RH could be quantitatively extracted by acidic ethanol when varying amounts of synthetic LH-RH were added to plasma. Immunoreactivity of LH-RH was preserved in plasma until 2 hr in the cold and gradually reduced thereafter. The plasma levels in LH-RH were 20 pg/ml or less in normal adults and not detectable in children. The aged males over 60 yr and postmenopausal women showed a tendency to have higher levels of plasma LH-RH. Plasma LH-RH level was significantly higher in midcycle than in follicular and luteal stages. The disappearance rate of LH-RH from the circulation after intravenous injection could be represented as half times of 4-6 min. Between 0.2-0.4% of the injected dose was excreted into urine within 1 hr. These results indicate that the determination of LH-RH might be a useful tool for elucidating hypothalamic-pituitary-gonad interactions.", "contents": "Radioimmunoassay for luteinizing hormone releasing hormone in plasma. A sensitive and specific double antibody radioimmunoassay has been developed capable of measuring LH-RH in extracted human plasma. Thyrotropin releasing hormone, lysine vasopressin and most of LH-RH analogues did not appear to affect the assay. Hypothalamic extract and some of the LH-RH analogues produced displacement curves which were parallel to that obtained with the synthetic LH-RH. Sensitivity of the radioimmunoassay was about 3 pg per assay tube. The coefficient of variation of intraassay was 6.4%, while that of interassay was 9.6%. Exogenous LH-RH could be quantitatively extracted by acidic ethanol when varying amounts of synthetic LH-RH were added to plasma. Immunoreactivity of LH-RH was preserved in plasma until 2 hr in the cold and gradually reduced thereafter. The plasma levels in LH-RH were 20 pg/ml or less in normal adults and not detectable in children. The aged males over 60 yr and postmenopausal women showed a tendency to have higher levels of plasma LH-RH. Plasma LH-RH level was significantly higher in midcycle than in follicular and luteal stages. The disappearance rate of LH-RH from the circulation after intravenous injection could be represented as half times of 4-6 min. Between 0.2-0.4% of the injected dose was excreted into urine within 1 hr. These results indicate that the determination of LH-RH might be a useful tool for elucidating hypothalamic-pituitary-gonad interactions."}
{"id": "PMID:1100365", "title": "[Studies on long-term irradiated rats using clomiphenedihydrogen citrate, LH and LH-RH].", "content": "The influence of clomiphene, LH and LH-RH on vaginal cycle and ovarian function in mature rats, which had been exposed to continuous light in order to block the cyclic sexual centre, was examined by different doses. While ovulation was obtained by administration of LH and LH-RH, clomiphene showed no effect. It may be assumed that the central mode of action of the \"ovulation inducing effect\" of clomiphene is mediated by stimulation of the cyclic sexual centre in the anterior hypothalamus.", "contents": "[Studies on long-term irradiated rats using clomiphenedihydrogen citrate, LH and LH-RH]. The influence of clomiphene, LH and LH-RH on vaginal cycle and ovarian function in mature rats, which had been exposed to continuous light in order to block the cyclic sexual centre, was examined by different doses. While ovulation was obtained by administration of LH and LH-RH, clomiphene showed no effect. It may be assumed that the central mode of action of the \"ovulation inducing effect\" of clomiphene is mediated by stimulation of the cyclic sexual centre in the anterior hypothalamus."}
{"id": "PMID:1100366", "title": "Ultrastructure of rat pituitary gonadotrophs after injection of synthetic luteinizing hormone releasing factor (LRF).", "content": "The effects of intracarotid injection of synthetic luteinizing hormone releasing factor (LRF) on the ultrastructure of pituitary gonadotrophs are reported. Chronically ovariectomized rats of the Sprague-Dawley strain pretreated with oestradiol and progesterone were sacrificed at zero time and at 30, 120 min after injection of 1 mug of synthetic LRF. A careful observation was made for evidence of granule extrusion from gonadotrophs following intracarotid injection of LRF, but no signs of granule extrusion were demonstrated. However, granule migration to the periphery of the cell was observed 30 min after LRF injection and the distribution of secretory granules recovered 2 hours after injection. The result of ultrastructural observations suggests that LRF plays an important role in releasing and synthesizing gonadotrophins in pituitary gonadotrophs. Further investigations should elucidate more clearly the morphologic aspect of mode of LRF action in pituitary gonadotrophs.", "contents": "Ultrastructure of rat pituitary gonadotrophs after injection of synthetic luteinizing hormone releasing factor (LRF). The effects of intracarotid injection of synthetic luteinizing hormone releasing factor (LRF) on the ultrastructure of pituitary gonadotrophs are reported. Chronically ovariectomized rats of the Sprague-Dawley strain pretreated with oestradiol and progesterone were sacrificed at zero time and at 30, 120 min after injection of 1 mug of synthetic LRF. A careful observation was made for evidence of granule extrusion from gonadotrophs following intracarotid injection of LRF, but no signs of granule extrusion were demonstrated. However, granule migration to the periphery of the cell was observed 30 min after LRF injection and the distribution of secretory granules recovered 2 hours after injection. The result of ultrastructural observations suggests that LRF plays an important role in releasing and synthesizing gonadotrophins in pituitary gonadotrophs. Further investigations should elucidate more clearly the morphologic aspect of mode of LRF action in pituitary gonadotrophs."}
{"id": "PMID:1100370", "title": "Lead in the environment - origins, pathways and sinks.", "content": "1. Lead is a natural constituent of the environment. 2. In many urban areas, the principal source of elevated lead in soils is the weathering of paint on houses. 3. Burning of lead in gasoline is the main source of lead in air in many areas. This lead is removed by sedimentation and in rainfall and snowfall. 4. Soil and sediments are the ultimate sinks for lead. 5. Man receives an average of about 300 mug of lead per day in his food, but the range is between 100 and 400 mug. 6. The natural concentration of lead in food resulting from the natural lead content of soil is a few tenths of a microgram per gram of wet plant material. 7. Present concentrations of lead in air do not measurably increase the lead content of the edible portion of most plants. 8. A several-fold increase in lead in soil does not measurably change the concentration of lead in plants. 9. Leafy portions of plants near busy highways contain higher concentrations of lead. 10. Rainwater does not appear to be a significant source of lead in crops.", "contents": "Lead in the environment - origins, pathways and sinks. 1. Lead is a natural constituent of the environment. 2. In many urban areas, the principal source of elevated lead in soils is the weathering of paint on houses. 3. Burning of lead in gasoline is the main source of lead in air in many areas. This lead is removed by sedimentation and in rainfall and snowfall. 4. Soil and sediments are the ultimate sinks for lead. 5. Man receives an average of about 300 mug of lead per day in his food, but the range is between 100 and 400 mug. 6. The natural concentration of lead in food resulting from the natural lead content of soil is a few tenths of a microgram per gram of wet plant material. 7. Present concentrations of lead in air do not measurably increase the lead content of the edible portion of most plants. 8. A several-fold increase in lead in soil does not measurably change the concentration of lead in plants. 9. Leafy portions of plants near busy highways contain higher concentrations of lead. 10. Rainwater does not appear to be a significant source of lead in crops."}
{"id": "PMID:1100371", "title": "Fluorescence studies on the interaction between yeast seryl-tRNA synthetase and its substrates.", "content": "Substrate-induced variations in the native fluorescence of seryl-tRNA synthetase from yeast have been used to evaluate the binding equilibria with its ligands. Binding of L-serine to the enzyme can be detected by equilibrium dialysis but not by fluorescence quenching. Hence, in contrast to ATP and tRNA(Ser), L-serine does not induce any fluorescence-sensitive conformational change of the synthetase. A comparison of the binding constants for ATP (2 X10(4) M-1) and seryl adenylate (1 x10(6) M-1) indicates that the activation reaction is mainly driven by the higher affinity of seryl adenylate to the enzyme. tRNA(Ser) and seryl-tRNA(Ser) though causing rather different maximal fluorescence quenching are bound to the enzyme with similar stoichiometry and association constant. The implications of this result are discussed with respect to the mechanism of the aminoacylation reaction. It is also shown that the enzyme clearly is capable of discriminating L-serine from other amino acids, ATP from other nucleotidetriphosphates and AMP, and tRNA(SER) from other tRNA species in the binding processes. The spectral properties, the effects of outside quenchers and the fluorescence decay times of the free enzyme and the enzyme-substrate complexes are discussed with respect to the intercalation hypothesis of H\u00e9l\u00e8ne.", "contents": "Fluorescence studies on the interaction between yeast seryl-tRNA synthetase and its substrates. Substrate-induced variations in the native fluorescence of seryl-tRNA synthetase from yeast have been used to evaluate the binding equilibria with its ligands. Binding of L-serine to the enzyme can be detected by equilibrium dialysis but not by fluorescence quenching. Hence, in contrast to ATP and tRNA(Ser), L-serine does not induce any fluorescence-sensitive conformational change of the synthetase. A comparison of the binding constants for ATP (2 X10(4) M-1) and seryl adenylate (1 x10(6) M-1) indicates that the activation reaction is mainly driven by the higher affinity of seryl adenylate to the enzyme. tRNA(Ser) and seryl-tRNA(Ser) though causing rather different maximal fluorescence quenching are bound to the enzyme with similar stoichiometry and association constant. The implications of this result are discussed with respect to the mechanism of the aminoacylation reaction. It is also shown that the enzyme clearly is capable of discriminating L-serine from other amino acids, ATP from other nucleotidetriphosphates and AMP, and tRNA(SER) from other tRNA species in the binding processes. The spectral properties, the effects of outside quenchers and the fluorescence decay times of the free enzyme and the enzyme-substrate complexes are discussed with respect to the intercalation hypothesis of H\u00e9l\u00e8ne."}
{"id": "PMID:1100372", "title": "A study of codon-dependent binding of aminoacyl-tRNA with the ribosomal 30-S subparticle of Escherichia coli. Determination of the active-particle fraction and binding constants in different media.", "content": "Titration of isolated Escherichia coli ribosomal 30-S particles with [14C]phenylalanyl-tRNA in the presence of poly(uridylic acid) was used for a quantitative assay of codon-dependent binding of aminoacyl-tRNA with the small ribosomal subparticle. The technique has allowed the estimation both of the fraction of \"active\" 30-S subparticles capable of forming the 30-S - poly(U) - phenylalanyl-tRNA complexes and the equilibrium constants of phenylalanyl-tRNA binding in different media. Heterogeneity of the ternary complexes formed has been revealed: at least two classes of complexes differing in stability have been observed. The stability of the 30-S - poly(U) - phenylalanyl-tRNA complexes has been shown to decrease with the lowering of the Mg2+ concentration, the increase of K+ concentration and the addition of urea. The stability of the complexes increases with the increase of Mg2+ concentration, with the addition of ethanol and decrease of temperature. It is demonstrated that the fraction of actively binding 30-S particles also varies in different medium conditions; it decreases with the increase of ionic strength (K+) and with the addition of urea, and increases with the increase of Mg2+ concentration and addition of ethanol.", "contents": "A study of codon-dependent binding of aminoacyl-tRNA with the ribosomal 30-S subparticle of Escherichia coli. Determination of the active-particle fraction and binding constants in different media. Titration of isolated Escherichia coli ribosomal 30-S particles with [14C]phenylalanyl-tRNA in the presence of poly(uridylic acid) was used for a quantitative assay of codon-dependent binding of aminoacyl-tRNA with the small ribosomal subparticle. The technique has allowed the estimation both of the fraction of \"active\" 30-S subparticles capable of forming the 30-S - poly(U) - phenylalanyl-tRNA complexes and the equilibrium constants of phenylalanyl-tRNA binding in different media. Heterogeneity of the ternary complexes formed has been revealed: at least two classes of complexes differing in stability have been observed. The stability of the 30-S - poly(U) - phenylalanyl-tRNA complexes has been shown to decrease with the lowering of the Mg2+ concentration, the increase of K+ concentration and the addition of urea. The stability of the complexes increases with the increase of Mg2+ concentration, with the addition of ethanol and decrease of temperature. It is demonstrated that the fraction of actively binding 30-S particles also varies in different medium conditions; it decreases with the increase of ionic strength (K+) and with the addition of urea, and increases with the increase of Mg2+ concentration and addition of ethanol."}
{"id": "PMID:1100373", "title": "The mode of action of pleuromutilin derivatives. Location and properties of the pleuromutilin binding site on Escherichia coli ribosomes.", "content": "Using equilibrium dialysis techniques it could be demonstrated that (a) the pleuromutilin derivative 14-deoxy-14[(2-diethylaminoethyl)-mercaptoacetoxy] dihydromutilin HCl binds to one site per ribosome specifically, (b) the binding constant is 1.3 times 10(7) M(-1) and (c) chloramphenicol and puromycin compete with binding of the pleuromutilin derivative. Similarly the nucleotides CpA and CpCpA also displace the unsaturated derivative of the above-mentioned pleuromutilin compound. These findings suggest that the ribosomal binding site for pleuromutilin overlaps with that for chloramphenicol and analogs of the 3'-terminus of a tRNA, like puromycin or the nucleotides CpA and CpCpA.", "contents": "The mode of action of pleuromutilin derivatives. Location and properties of the pleuromutilin binding site on Escherichia coli ribosomes. Using equilibrium dialysis techniques it could be demonstrated that (a) the pleuromutilin derivative 14-deoxy-14[(2-diethylaminoethyl)-mercaptoacetoxy] dihydromutilin HCl binds to one site per ribosome specifically, (b) the binding constant is 1.3 times 10(7) M(-1) and (c) chloramphenicol and puromycin compete with binding of the pleuromutilin derivative. Similarly the nucleotides CpA and CpCpA also displace the unsaturated derivative of the above-mentioned pleuromutilin compound. These findings suggest that the ribosomal binding site for pleuromutilin overlaps with that for chloramphenicol and analogs of the 3'-terminus of a tRNA, like puromycin or the nucleotides CpA and CpCpA."}
{"id": "PMID:1100374", "title": "The mechanism of template activation by exonuclease V.", "content": "In crude extracts from Escherichia coli cells the ATP-dependent exonuclease V was found to be most active in converting double-stranded DNA into a suitable template for DNA polymerase. This phenomenon was studied in some detail with isolated exonuclease V and T7 DNA polymerase. We found that, at ATP concentrations arount 1 mM, the exonuclease produces a broad spectrum of DNA fragments. One class of fragments is largely single stranded with hydrogen-bonded small primer sequences. These structures allow the synthesis of remarkably homogeneous polynucleotide strands by T7 DNA polymerase.", "contents": "The mechanism of template activation by exonuclease V. In crude extracts from Escherichia coli cells the ATP-dependent exonuclease V was found to be most active in converting double-stranded DNA into a suitable template for DNA polymerase. This phenomenon was studied in some detail with isolated exonuclease V and T7 DNA polymerase. We found that, at ATP concentrations arount 1 mM, the exonuclease produces a broad spectrum of DNA fragments. One class of fragments is largely single stranded with hydrogen-bonded small primer sequences. These structures allow the synthesis of remarkably homogeneous polynucleotide strands by T7 DNA polymerase."}
{"id": "PMID:1100375", "title": "Molecular morphology of ribosomes. Iodination of Escherichia coli ribosomal proteins with solid-state lactoperoxidase.", "content": "Using either soluble or solid-state lactoperoxidase, a comparison was made between the enzymic iodination of ribosomal proteins iodinated as 30-S and 50-S subunits or as 70-S monosomes. Proteins S7, S11 and S12 of the 30-S subunit and proteins L2, L11, L26 and L28 of the 50-S subunit were labelled to a greater extent in isolated particles than in the 70-S ribosome. In contrast, proteins S4, S19 and S20 were labelled to a lesser extent in the isolated subunit. No significant differences were observed in the iodination patterns of ribosomes iodinated in the presence of soluble lactoperoxidase and those iodinated in the presence of lactoperoxidase bound to Sepharose 4B. It is suggested that the 30-S subunit undergoes a conformational change during its association with the 50-S subunit to form a 70-S monosome. Implications from results obtained with solid-state lactoperoxidase-catalyzed iodination of ribosomal proteins are also discussed.", "contents": "Molecular morphology of ribosomes. Iodination of Escherichia coli ribosomal proteins with solid-state lactoperoxidase. Using either soluble or solid-state lactoperoxidase, a comparison was made between the enzymic iodination of ribosomal proteins iodinated as 30-S and 50-S subunits or as 70-S monosomes. Proteins S7, S11 and S12 of the 30-S subunit and proteins L2, L11, L26 and L28 of the 50-S subunit were labelled to a greater extent in isolated particles than in the 70-S ribosome. In contrast, proteins S4, S19 and S20 were labelled to a lesser extent in the isolated subunit. No significant differences were observed in the iodination patterns of ribosomes iodinated in the presence of soluble lactoperoxidase and those iodinated in the presence of lactoperoxidase bound to Sepharose 4B. It is suggested that the 30-S subunit undergoes a conformational change during its association with the 50-S subunit to form a 70-S monosome. Implications from results obtained with solid-state lactoperoxidase-catalyzed iodination of ribosomal proteins are also discussed."}
{"id": "PMID:1100376", "title": "Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography.", "content": "DNA has been covalently linked to insoluble matrices of agarose (Sepharose) in high yield using cyanogen bromide activation. Both double-stranded and single-stranded DNA have been coupled with yields up to 225 nmol/mg dry weight Sepharose or 3-8 mumol nucleotide phosphate/ml bed volume. The DNA-Sepharose has been used for (a) the affinity chromatography of various enzymes (Escherichia coli DNA polymerase I and RNA polymerase) from crude extracts or after initial purification steps, resulting in high yields and degrees of purification, and for (b) nucleic acid hybridization. The DNA-Sepharose is stable to high temperature, prolonged storage, and in the case of single-stranded DNA, can be washed with NaOH to destroy nuclease activity and to release any digested oligonucleotides or mononucleotides.", "contents": "Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography. DNA has been covalently linked to insoluble matrices of agarose (Sepharose) in high yield using cyanogen bromide activation. Both double-stranded and single-stranded DNA have been coupled with yields up to 225 nmol/mg dry weight Sepharose or 3-8 mumol nucleotide phosphate/ml bed volume. The DNA-Sepharose has been used for (a) the affinity chromatography of various enzymes (Escherichia coli DNA polymerase I and RNA polymerase) from crude extracts or after initial purification steps, resulting in high yields and degrees of purification, and for (b) nucleic acid hybridization. The DNA-Sepharose is stable to high temperature, prolonged storage, and in the case of single-stranded DNA, can be washed with NaOH to destroy nuclease activity and to release any digested oligonucleotides or mononucleotides."}
{"id": "PMID:1100377", "title": "Sterol biosynthesis in yeast. 3-Hydorxy-3-methylglutaryl-Coenzyme A reductase as a regulatory enzyme.", "content": "Anaerobically and aerobically grown yeast contains 3-hydroxy-3-methylglutaryl-CoA reductase, which is located in the mitochondrial fraction of the cell. Anaerobically grown yeast has a low specific activity of 3-hydroxy-3-methylglutaryl-CoA reductase and a low sterol content. Aeration of this yeast in buffer, without growth, results in an increase in the specific activity of the enzyme, which is paralleled by an increase in the sterol content. This induction has an oscillatory profile with yeast grown anaerobically for 24 h and a linear pattern with cells grown anaerobically for 72 h. With the latter type of yeast, glucose is necessary for an induction, whereas with the other yeast an induction occurs with and without glucose. By an anaerobic incubation in buffer of the yeast grown anaerobically for 24 h, the oscillatory profile can be transformed into a linear one. The extent of induction of the reductase is strictly dependent on the concentration of glucose present. Sterols increase in whole cells, but they do not increase in the mitochondrial fraction. The induction of 3-hydroxy-3-methylglutaryl-CoA reductase is strongly inhibited by cycloheximide, but is not affected by chloramphenicol. The induction of the enzyme is closely connected with the glucose metabolism of the cell; fructose, mannose, and ethanol can also induce the reductase.", "contents": "Sterol biosynthesis in yeast. 3-Hydorxy-3-methylglutaryl-Coenzyme A reductase as a regulatory enzyme. Anaerobically and aerobically grown yeast contains 3-hydroxy-3-methylglutaryl-CoA reductase, which is located in the mitochondrial fraction of the cell. Anaerobically grown yeast has a low specific activity of 3-hydroxy-3-methylglutaryl-CoA reductase and a low sterol content. Aeration of this yeast in buffer, without growth, results in an increase in the specific activity of the enzyme, which is paralleled by an increase in the sterol content. This induction has an oscillatory profile with yeast grown anaerobically for 24 h and a linear pattern with cells grown anaerobically for 72 h. With the latter type of yeast, glucose is necessary for an induction, whereas with the other yeast an induction occurs with and without glucose. By an anaerobic incubation in buffer of the yeast grown anaerobically for 24 h, the oscillatory profile can be transformed into a linear one. The extent of induction of the reductase is strictly dependent on the concentration of glucose present. Sterols increase in whole cells, but they do not increase in the mitochondrial fraction. The induction of 3-hydroxy-3-methylglutaryl-CoA reductase is strongly inhibited by cycloheximide, but is not affected by chloramphenicol. The induction of the enzyme is closely connected with the glucose metabolism of the cell; fructose, mannose, and ethanol can also induce the reductase."}
{"id": "PMID:1100378", "title": "Mechanism of 2-deoxy-D-glucose inhibition of cell-wall polysaccharide and glycoprotein biosyntheses in Saccharomyces cerevisiae.", "content": "The mechanism of inhibition by 2-deoxy-D-glucose of the synthesis of yeast wall polysaccharides and glycoproteins was investigated in Saccharomyces cerevisiae cells and protoplasts. The extent of the inhibition of mannan and glucan synthesis was found to be dependent on whether glucose or mannose was used as the carbon source in the medium. During growth on glucose, 2-deoxy-D-glucose inhibited more intensively mannan than glucan formation. Biosynthesis of wall glucan was strongly suppressed in mannose medium. Selective incorporation of 2-deoxy-D-glucose occurred into that polysaccharide, synthesis of which was more inhibited under given conditions. Suggestive evidence has been obtained that the decisive factor for the proportion of glucan and mannan in the walls is the direction of glucose 6-phosphate/mannose 6-phosphate interconversion dependent on the exogeneous hexose. No close correlation was found between the inhibition of mannan synthesis and the appearance of the mannan-protein enzymes invertase and acid phosphatase. Effect of 2-deoxy-D-glucose was therefore investigated on the parallel synthesis of protein, mannan and several extracellular and intracellular enzymes in protoplasts grown on glucose and mannose. The results obtained pointed out that the hindrance of the secretion of mannan-protein enzymes is of a complex nature and related more to the inhibition of synthesis of the protein moiety than to the inhibition of glycosylation. Synthesis of several enzymes was found to be a subject of a metabolic control by 2-deoxy-D-glucose or its metabolites.", "contents": "Mechanism of 2-deoxy-D-glucose inhibition of cell-wall polysaccharide and glycoprotein biosyntheses in Saccharomyces cerevisiae. The mechanism of inhibition by 2-deoxy-D-glucose of the synthesis of yeast wall polysaccharides and glycoproteins was investigated in Saccharomyces cerevisiae cells and protoplasts. The extent of the inhibition of mannan and glucan synthesis was found to be dependent on whether glucose or mannose was used as the carbon source in the medium. During growth on glucose, 2-deoxy-D-glucose inhibited more intensively mannan than glucan formation. Biosynthesis of wall glucan was strongly suppressed in mannose medium. Selective incorporation of 2-deoxy-D-glucose occurred into that polysaccharide, synthesis of which was more inhibited under given conditions. Suggestive evidence has been obtained that the decisive factor for the proportion of glucan and mannan in the walls is the direction of glucose 6-phosphate/mannose 6-phosphate interconversion dependent on the exogeneous hexose. No close correlation was found between the inhibition of mannan synthesis and the appearance of the mannan-protein enzymes invertase and acid phosphatase. Effect of 2-deoxy-D-glucose was therefore investigated on the parallel synthesis of protein, mannan and several extracellular and intracellular enzymes in protoplasts grown on glucose and mannose. The results obtained pointed out that the hindrance of the secretion of mannan-protein enzymes is of a complex nature and related more to the inhibition of synthesis of the protein moiety than to the inhibition of glycosylation. Synthesis of several enzymes was found to be a subject of a metabolic control by 2-deoxy-D-glucose or its metabolites."}
{"id": "PMID:1100379", "title": "Quantitative binding of antibiotics to ribosomes from a yeast mutant altered on the peptidyl-transferase center.", "content": "Quantitative binding studies of [G-3H]anisomycin and [acetyl-14C]trichodermin to sensitive and resistant 80-S ribosomes from yeasts are described in this work. A single mutation, most probably affecting the ribosome peptidyl transferase centre, appears to have pleiotropic effects on the ribosome leading to resistance to trichodermin and anisomycin and to an increased sensitivity to sparsomycin. Resistance to trichodermin is due to a reduced affinity of ribosomes from the mutant for the antibiotic. Ribosomes from the sensitive strain (Y 1661 bind [acetyl-14C]trichodermin with a dissociation constant of 0.99 muM while those from the resistant one (TR1) bind [acetyl-14C]trichodermin with a dissociation constant of 15.4 muM. Similar results are obtained when the binding of [acetyl-14C]trichodermin to Y 166 and TR1 60-S subunits is studied. The mutant TR1 is also resistant to anisomycin. Although trichodermin and anisomycin bind to the ribosome at mutually exclusive sites, the higher affinity binding of [G-3H]anisomycin that is responsible for the inhibition of the peptidyl transferase center is practically identical for Y 166 and TR1 ribosomes. Therefore, the mutation in the ribosome leading to resistance to trichodermin and anisomycin decreases the affinity for trichodermin but not for anisomycin. Trichodermin, trichothecin and fusarenon X inhibit the binding of [G-3H]anisomycin to TR1 ribosomes to a lower extent than to Y 166 ribosomes, suggesting that the resistance of TR1 ribosomes to the effects of trichothecin and fusarenon X is caused by a decrease in the affinity of the ribosomes for these drugs, as was seen with trichodermin. On the other hand, verrucarin A inhibits [G-3H]anisomycin binding to Y 166 and TR1 ribosomes to a similar extent and therefore its affinity for the ribosome does not appear to be affected by the mutation leading to resistance. Trichothecin, trichodermin and fusarenon X appear to have a common binding site on the 60-S ribosomal subunits, which overlaps or is closely linked to the binding sites of anisomycin and verrucarin A.", "contents": "Quantitative binding of antibiotics to ribosomes from a yeast mutant altered on the peptidyl-transferase center. Quantitative binding studies of [G-3H]anisomycin and [acetyl-14C]trichodermin to sensitive and resistant 80-S ribosomes from yeasts are described in this work. A single mutation, most probably affecting the ribosome peptidyl transferase centre, appears to have pleiotropic effects on the ribosome leading to resistance to trichodermin and anisomycin and to an increased sensitivity to sparsomycin. Resistance to trichodermin is due to a reduced affinity of ribosomes from the mutant for the antibiotic. Ribosomes from the sensitive strain (Y 1661 bind [acetyl-14C]trichodermin with a dissociation constant of 0.99 muM while those from the resistant one (TR1) bind [acetyl-14C]trichodermin with a dissociation constant of 15.4 muM. Similar results are obtained when the binding of [acetyl-14C]trichodermin to Y 166 and TR1 60-S subunits is studied. The mutant TR1 is also resistant to anisomycin. Although trichodermin and anisomycin bind to the ribosome at mutually exclusive sites, the higher affinity binding of [G-3H]anisomycin that is responsible for the inhibition of the peptidyl transferase center is practically identical for Y 166 and TR1 ribosomes. Therefore, the mutation in the ribosome leading to resistance to trichodermin and anisomycin decreases the affinity for trichodermin but not for anisomycin. Trichodermin, trichothecin and fusarenon X inhibit the binding of [G-3H]anisomycin to TR1 ribosomes to a lower extent than to Y 166 ribosomes, suggesting that the resistance of TR1 ribosomes to the effects of trichothecin and fusarenon X is caused by a decrease in the affinity of the ribosomes for these drugs, as was seen with trichodermin. On the other hand, verrucarin A inhibits [G-3H]anisomycin binding to Y 166 and TR1 ribosomes to a similar extent and therefore its affinity for the ribosome does not appear to be affected by the mutation leading to resistance. Trichothecin, trichodermin and fusarenon X appear to have a common binding site on the 60-S ribosomal subunits, which overlaps or is closely linked to the binding sites of anisomycin and verrucarin A."}
{"id": "PMID:1100380", "title": "Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms.", "content": "Ions of low molecular weight like metal cations and basic amines are present in lipopolysaccharides regardless of the isolation procedure employed. They are present in salt form with the acidic groups of the molecule and, partly, bound by chelation. Electrodialysis which removed a large proportion of these basic materials led to acidic lipopolysaccharides often with reduced solubility. Electrodialyzed lopopolysaccharides could be rendered soluble by neutralizing with alkali or with a basic amine. Depending on the base employed for neutralization preparations were obtained which showed in water distinct differences in solubility, viscosity and opalescence. These differences were related to differences in the sedimentation coefficients of the various salt forms. Neutralization with triethylamine led in all cases to highly soluble preparations with low sedimentation coefficients, while, on the other hand, neutralization with Mg(OH)2 led in most cases to insoluble preparations. The acidic lipopolysaccharides obtained by electrodialysis deteriorate on storing in a freeze-dried form. On heating in distilled water autohydrolysis occurs and free lipid A is liberated. The lipid A which is so far known as a water-insoluble material showed increased solubility when prepared from electrodialyzed lipopolysaccharides.", "contents": "Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Ions of low molecular weight like metal cations and basic amines are present in lipopolysaccharides regardless of the isolation procedure employed. They are present in salt form with the acidic groups of the molecule and, partly, bound by chelation. Electrodialysis which removed a large proportion of these basic materials led to acidic lipopolysaccharides often with reduced solubility. Electrodialyzed lopopolysaccharides could be rendered soluble by neutralizing with alkali or with a basic amine. Depending on the base employed for neutralization preparations were obtained which showed in water distinct differences in solubility, viscosity and opalescence. These differences were related to differences in the sedimentation coefficients of the various salt forms. Neutralization with triethylamine led in all cases to highly soluble preparations with low sedimentation coefficients, while, on the other hand, neutralization with Mg(OH)2 led in most cases to insoluble preparations. The acidic lipopolysaccharides obtained by electrodialysis deteriorate on storing in a freeze-dried form. On heating in distilled water autohydrolysis occurs and free lipid A is liberated. The lipid A which is so far known as a water-insoluble material showed increased solubility when prepared from electrodialyzed lipopolysaccharides."}
{"id": "PMID:1100381", "title": "Regulation of protein secretion and metabolism in rat salivary glands. Effects of norepinephrine and carbachol on the glycogenolysis in submaxillary glands.", "content": "Carbachol and norepinephrine induce glycogen breakdown in rat submaxillary glands incubated in vitro. These effects are suppressed by specific inhibitors of cholinergic receptors (atropine, a muscarinic blocker) and of adrenergic receptors (propranolol, a beta-blocker and phentolamine, an alpha-blocker). The beta-adrenergic effect seems to be mediated through the adenosine 3':5'-monophosphate system, whereas the alpha-adrenergic and cholinergic effects are dependent of Ca2+ influx into cell. In the two latter systems, it is suggested that a direct activation of phosphorylase b kinase by calcium takes place. These data clearly indicate that glycogenolysis in rat submaxillary glands in vitro can be independently affected by sympathetic and parasympathetic agents via specific receptors.", "contents": "Regulation of protein secretion and metabolism in rat salivary glands. Effects of norepinephrine and carbachol on the glycogenolysis in submaxillary glands. Carbachol and norepinephrine induce glycogen breakdown in rat submaxillary glands incubated in vitro. These effects are suppressed by specific inhibitors of cholinergic receptors (atropine, a muscarinic blocker) and of adrenergic receptors (propranolol, a beta-blocker and phentolamine, an alpha-blocker). The beta-adrenergic effect seems to be mediated through the adenosine 3':5'-monophosphate system, whereas the alpha-adrenergic and cholinergic effects are dependent of Ca2+ influx into cell. In the two latter systems, it is suggested that a direct activation of phosphorylase b kinase by calcium takes place. These data clearly indicate that glycogenolysis in rat submaxillary glands in vitro can be independently affected by sympathetic and parasympathetic agents via specific receptors."}
{"id": "PMID:1100382", "title": "tRNA conformation and magnesium binding. A study of a yeast phenylalanine-specific tRNA by a fluorescent indicator and differential melting curves.", "content": "The binding of Mg2+ to tRNAPhe (yeast) in three conformational states was studied at 10, 30, 45, and 70 degrees C by the fluorescence indicator 8-hydroxyquinoline 5-sulphonic acid in the presence of 0.032 M monovalent cations (Na+). At temperatures below those characteristic for early melting (completely folded tRNA) the Scatchard plots are biphasic. They were well fitted by two classes of noninteracting binding sites with stability constants independent of temperature (KA = 9X10(4), KB = 6X10(3) M-1). In partially unfolded tRNA the strong binding process is co-operative. A single class of weak sites was found in the statistically coiled conformation at 70 degrees C (KB = 3.3X103 M-1). The total number of binding sites is 23 +/- 5; differences for the folded and unfolded conformations are smaller than 1. The influence of Mg2+ on the stability of the conformational elements of tRNAPhe (yeast) and its CCA-half (i.e. nucleotides 38--76) was determined by differential ultraviolet absorbance and depolarisation melting curves using the fluorescence of the Y base. Tertiary structure corresponding to early melting is stabilized by strongly bound Mg2+, whereas all other melting transitions are only influenced by Mg2+ bound at weak sites. The stability constants of tertiary structure obtained from the melting experiments can quantitatively be described by assuming that 5 +/- 1 non-interacting strong sites as characterized by the fluorescence titrations are converted to weak sites upon unfolding of the tertiary structure. Co-operative interaction of Mg2+ with the 5 strong sites in the folded conformation of tRNA can be ruled out. Strong binding of Mg2+ to completely folded tRNA does not produce a conformational transition changing ultraviolet absorbance, circular dichroism and sedimentation coefficient.", "contents": "tRNA conformation and magnesium binding. A study of a yeast phenylalanine-specific tRNA by a fluorescent indicator and differential melting curves. The binding of Mg2+ to tRNAPhe (yeast) in three conformational states was studied at 10, 30, 45, and 70 degrees C by the fluorescence indicator 8-hydroxyquinoline 5-sulphonic acid in the presence of 0.032 M monovalent cations (Na+). At temperatures below those characteristic for early melting (completely folded tRNA) the Scatchard plots are biphasic. They were well fitted by two classes of noninteracting binding sites with stability constants independent of temperature (KA = 9X10(4), KB = 6X10(3) M-1). In partially unfolded tRNA the strong binding process is co-operative. A single class of weak sites was found in the statistically coiled conformation at 70 degrees C (KB = 3.3X103 M-1). The total number of binding sites is 23 +/- 5; differences for the folded and unfolded conformations are smaller than 1. The influence of Mg2+ on the stability of the conformational elements of tRNAPhe (yeast) and its CCA-half (i.e. nucleotides 38--76) was determined by differential ultraviolet absorbance and depolarisation melting curves using the fluorescence of the Y base. Tertiary structure corresponding to early melting is stabilized by strongly bound Mg2+, whereas all other melting transitions are only influenced by Mg2+ bound at weak sites. The stability constants of tertiary structure obtained from the melting experiments can quantitatively be described by assuming that 5 +/- 1 non-interacting strong sites as characterized by the fluorescence titrations are converted to weak sites upon unfolding of the tertiary structure. Co-operative interaction of Mg2+ with the 5 strong sites in the folded conformation of tRNA can be ruled out. Strong binding of Mg2+ to completely folded tRNA does not produce a conformational transition changing ultraviolet absorbance, circular dichroism and sedimentation coefficient."}
{"id": "PMID:1100383", "title": "Isolation and partial characterisation of the relaxation protein from nuclei of cultured mouse and human cells.", "content": "A protein, called relaxation protein because of its ability to remove superhelical turns in closed-circular DNA, has been isolated and partially characterized from the nuclei of LA9 mouse and HeLa cells. The purification was facilitated by an assay method, with PM2 DNA, which used the fluorescence enhancement of the intercalating dye ethidium bromide upon binding to the closed-circular DNA. The amount of dye bound depends upon the degree of the superhelix density of the DNA. The relaxation products were analysed by the buoyant separation method in CsCl containing ethidium bromide and were shown to be completely relaxed. The purification resulted in a single band in a dodecylsulfate gel electrophoresis with an apparent molecular weight of 37000. The pH optimum is 7.0 and the optimal salt concentration is 0.2 M NaCl. The relaxation protein removes negative as well as positive supercoils, the latter generated by the interaction of ethidium bromide with closed-circular DNA. Relaxation of positive supercoils results, after removal of the dye, in the formation of molecules with superhelix densities exceeding that of native PM2 DNA (0.054). The highest negative superhelix density observed was -0.098 +/- 0.001. The corresponding positive superhelix density has been calculated to be + 0.023. A nicking--swivelling--closing mechanism is postulated, but nicked intermediates have so far not been demonstrated. The relaxation protein is not inhibited by known mammalian endonuclease I inhibitors, except for denatured DNA, and does not possess a conventional polynucleotide ligase activity. The relaxation activity was found to be predominantly in the nuclei, with only small amounts present in the cytoplasm and mitochondria. The biological function of transient swivels induced by the relaxation protein is not known. However, transient swivels are considered necessary or useful in the replication of closed-circular DNA or long linear DNA, respectively. Relaxation protein could replace the combined action of an endonuclease and a ligase ahead of the replication fork. Alternatively, transient swivels could be involved in the transcription process.", "contents": "Isolation and partial characterisation of the relaxation protein from nuclei of cultured mouse and human cells. A protein, called relaxation protein because of its ability to remove superhelical turns in closed-circular DNA, has been isolated and partially characterized from the nuclei of LA9 mouse and HeLa cells. The purification was facilitated by an assay method, with PM2 DNA, which used the fluorescence enhancement of the intercalating dye ethidium bromide upon binding to the closed-circular DNA. The amount of dye bound depends upon the degree of the superhelix density of the DNA. The relaxation products were analysed by the buoyant separation method in CsCl containing ethidium bromide and were shown to be completely relaxed. The purification resulted in a single band in a dodecylsulfate gel electrophoresis with an apparent molecular weight of 37000. The pH optimum is 7.0 and the optimal salt concentration is 0.2 M NaCl. The relaxation protein removes negative as well as positive supercoils, the latter generated by the interaction of ethidium bromide with closed-circular DNA. Relaxation of positive supercoils results, after removal of the dye, in the formation of molecules with superhelix densities exceeding that of native PM2 DNA (0.054). The highest negative superhelix density observed was -0.098 +/- 0.001. The corresponding positive superhelix density has been calculated to be + 0.023. A nicking--swivelling--closing mechanism is postulated, but nicked intermediates have so far not been demonstrated. The relaxation protein is not inhibited by known mammalian endonuclease I inhibitors, except for denatured DNA, and does not possess a conventional polynucleotide ligase activity. The relaxation activity was found to be predominantly in the nuclei, with only small amounts present in the cytoplasm and mitochondria. The biological function of transient swivels induced by the relaxation protein is not known. However, transient swivels are considered necessary or useful in the replication of closed-circular DNA or long linear DNA, respectively. Relaxation protein could replace the combined action of an endonuclease and a ligase ahead of the replication fork. Alternatively, transient swivels could be involved in the transcription process."}
{"id": "PMID:1100384", "title": "Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases.", "content": "Complexes between tRNAPhe (yeast), tRNASer (yeast) and tRNATyr (Escherichia coli) and their cognate aminoacyl-tRNA synthetases have been studied by sedimentation velocity runs in an analytical ultracentrifuge. The amount of complex formation was determined by the absorption and the sedimentation coefficients of the fast-moving boundary in the presence of excess tRNA or excess synthetase respectively. The same method has been applied to unspecific combinations of tRNAs and synthetases. Inactive material of tRNA or synthetase does not influence the results. 1. Two moles of tRNAPhe can be bound to one mole of phenylalanyl-tRNA synthetase with a binding constant greater than 10(6) M-1. The binding constants for both tRNAs are very similar; the binding sites are independent of each other. Omission of Mg2+ does not prevent binding. 2. Two moles of tRNASer can be bound to one mole of Seryl-tRNA synthetase; the binding of the first and second tRNA is non-equivalent, K1 greater than 10(6) M-1, K2 is determined to be 1.3 X 10(5) M-1 at pH 7.2. Omission of Mg2+ prevents complex formation. 3. Tyrosyl-tRNA synthetase behaves very similarly to seryl-tRNA synthetase. The binding constant for the weakly bound tRNA is 2.3 X 10(5) M-1 at pH 7.2, and 2.5 X 10(6) M-1 at pH 6.0. No complexes are observed in the absence of Mg2+. 4. Unspecific binding was only obtained with phenylalanyl-tRNA synthetase. It binds tRNASer (yeast), tRNAAla (yeast) and tRNATyr (E. coli) with a binding constant about 100 times lower compared to its cognate tRNA. The binding data are discussed with respect to the tertiary structure of the tRNAs, the subunit structure of the synthetases and the possible physical basis for the non-equivalence of binding sites.", "contents": "Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases. Complexes between tRNAPhe (yeast), tRNASer (yeast) and tRNATyr (Escherichia coli) and their cognate aminoacyl-tRNA synthetases have been studied by sedimentation velocity runs in an analytical ultracentrifuge. The amount of complex formation was determined by the absorption and the sedimentation coefficients of the fast-moving boundary in the presence of excess tRNA or excess synthetase respectively. The same method has been applied to unspecific combinations of tRNAs and synthetases. Inactive material of tRNA or synthetase does not influence the results. 1. Two moles of tRNAPhe can be bound to one mole of phenylalanyl-tRNA synthetase with a binding constant greater than 10(6) M-1. The binding constants for both tRNAs are very similar; the binding sites are independent of each other. Omission of Mg2+ does not prevent binding. 2. Two moles of tRNASer can be bound to one mole of Seryl-tRNA synthetase; the binding of the first and second tRNA is non-equivalent, K1 greater than 10(6) M-1, K2 is determined to be 1.3 X 10(5) M-1 at pH 7.2. Omission of Mg2+ prevents complex formation. 3. Tyrosyl-tRNA synthetase behaves very similarly to seryl-tRNA synthetase. The binding constant for the weakly bound tRNA is 2.3 X 10(5) M-1 at pH 7.2, and 2.5 X 10(6) M-1 at pH 6.0. No complexes are observed in the absence of Mg2+. 4. Unspecific binding was only obtained with phenylalanyl-tRNA synthetase. It binds tRNASer (yeast), tRNAAla (yeast) and tRNATyr (E. coli) with a binding constant about 100 times lower compared to its cognate tRNA. The binding data are discussed with respect to the tertiary structure of the tRNAs, the subunit structure of the synthetases and the possible physical basis for the non-equivalence of binding sites."}
{"id": "PMID:1100385", "title": "[Mechanism for the condensation reaction of fatty-acid biosynthesis (author's transl)].", "content": "The spontaneous hydrogen-deuterium exchange of the methylene group of malonyl-thioesters was investigated by nuclear-magnetic-resonance (NMR) spectroscopy using the model compound S-malonyl-N-acetylcysteamine. The half life of the methylene proteins is 12 to 16 min in 0.1 M K-phosphate buffer at pH 6.5 to 7.0 at 25 degrees C, the conditions of maximal activity of fatty acid synthetase from yeast. Proton catalysis was used for the quick preparation of deuterium- and tritium-labeled malonylthioesters. Compared with malonyl-CoA, dideutero-malonyl-CoA had no primary isotope effect on the reaction velocity of the yeast enzyme catalysed fatty acid synthesis, in which the rate limiting step is the condensation reaction. Although deuterium oxide had a solvent isotope effect, there was no difference in reaction velocities between malonyl CoA and dideuteromalonyl CoA in deuterium oxide. The condensation reaction was investiaged separately from the overall fatty acid synthesis using beta-ketoacyl-acyl-carrier-protein (ACP) synthetase (condensing enzyme) of Escherichia coli. The condensation reaction with deuteromalonyl-ACP had no kinetic isotope effect, in agreement with the observations on the overall reaction. However, in this case no solvent isotope effect was observed with 2H2O. When the condensation reaction was carried out in the presence of tritiated water, there was no incorporation of label into the reaction product acetoacetyl-thioester, excluding proton exchange with the solvent. The results exclude a mechanism for the condensation reaction involving a malonyl carbanion and its acylation as intermediates in the sense of an organic-chemical malonic ester synthesis, and they indicate that the condensation reaction follows a concerted mechanism: The formation of the new carbon-carbon bond is coupled with the cleavage of the carboxyl bond of the malonyl group.", "contents": "[Mechanism for the condensation reaction of fatty-acid biosynthesis (author's transl)]. The spontaneous hydrogen-deuterium exchange of the methylene group of malonyl-thioesters was investigated by nuclear-magnetic-resonance (NMR) spectroscopy using the model compound S-malonyl-N-acetylcysteamine. The half life of the methylene proteins is 12 to 16 min in 0.1 M K-phosphate buffer at pH 6.5 to 7.0 at 25 degrees C, the conditions of maximal activity of fatty acid synthetase from yeast. Proton catalysis was used for the quick preparation of deuterium- and tritium-labeled malonylthioesters. Compared with malonyl-CoA, dideutero-malonyl-CoA had no primary isotope effect on the reaction velocity of the yeast enzyme catalysed fatty acid synthesis, in which the rate limiting step is the condensation reaction. Although deuterium oxide had a solvent isotope effect, there was no difference in reaction velocities between malonyl CoA and dideuteromalonyl CoA in deuterium oxide. The condensation reaction was investiaged separately from the overall fatty acid synthesis using beta-ketoacyl-acyl-carrier-protein (ACP) synthetase (condensing enzyme) of Escherichia coli. The condensation reaction with deuteromalonyl-ACP had no kinetic isotope effect, in agreement with the observations on the overall reaction. However, in this case no solvent isotope effect was observed with 2H2O. When the condensation reaction was carried out in the presence of tritiated water, there was no incorporation of label into the reaction product acetoacetyl-thioester, excluding proton exchange with the solvent. The results exclude a mechanism for the condensation reaction involving a malonyl carbanion and its acylation as intermediates in the sense of an organic-chemical malonic ester synthesis, and they indicate that the condensation reaction follows a concerted mechanism: The formation of the new carbon-carbon bond is coupled with the cleavage of the carboxyl bond of the malonyl group."}
{"id": "PMID:1100386", "title": "Quaternary structure of polynucleotide phosphorylase from Escherichia coli: evidence of a complex between two types of polypeptide chains.", "content": "A new form of polynucleotide phosphorylase containing alpha and beta subunits was isolated (form B) by purification without preparative electrophoresis; this form was compared to the enzyme obtained by preparative electrophoresis purification (form A). The Stokes radius of these two forms are very different: 6.4 nm for form A and 8.7 - 9.0 nm for form B; on the other hand the sedimentation coefficients are close: 8.9 S and 9.9 S respectively. Such a result suggests that form B is very asymmetric. The apparent molecular weights, calculated from the Stokes radii and from the sedimentation coefficients, are approximately 365000 for form B and 252000 for form A. The latter is homogeneous on polyacrylamide gel, whereas the former yields two components, one of which behaves similarly to form A. Finally, whereas form A is composed of only one type of subunit, alpha, form B contains two types of chains: alpha (Mr 86000 +/- 5000) and beta (Mr 48000 +/- 2000) in stoichiometric proportions. From these results we believe that one should assume for form B the existence of a complex between form A and beta chains; the role of the latter still remains to be specified.", "contents": "Quaternary structure of polynucleotide phosphorylase from Escherichia coli: evidence of a complex between two types of polypeptide chains. A new form of polynucleotide phosphorylase containing alpha and beta subunits was isolated (form B) by purification without preparative electrophoresis; this form was compared to the enzyme obtained by preparative electrophoresis purification (form A). The Stokes radius of these two forms are very different: 6.4 nm for form A and 8.7 - 9.0 nm for form B; on the other hand the sedimentation coefficients are close: 8.9 S and 9.9 S respectively. Such a result suggests that form B is very asymmetric. The apparent molecular weights, calculated from the Stokes radii and from the sedimentation coefficients, are approximately 365000 for form B and 252000 for form A. The latter is homogeneous on polyacrylamide gel, whereas the former yields two components, one of which behaves similarly to form A. Finally, whereas form A is composed of only one type of subunit, alpha, form B contains two types of chains: alpha (Mr 86000 +/- 5000) and beta (Mr 48000 +/- 2000) in stoichiometric proportions. From these results we believe that one should assume for form B the existence of a complex between form A and beta chains; the role of the latter still remains to be specified."}
{"id": "PMID:1100387", "title": "The modification of DNA-dependent RNA polymerase from Escherichia coli by an alkylating derivative of rifamycin SV.", "content": "3-(2-Bromo[1-14C]acetamidoethyl)-thio-rifamycin SV, abbreviated BrAcNEtS-Rif, and alkylating derivative of rifamycin SV was synthesized. A four-fold excess of BrAcNEtS-Rif inhibited the enzymic activity of RNA polymerase from Escherichia coli to 97%. Incubation of RNA polymerase with Br[14C]AcNEtS-Rif led to covalent substitution. The reaction of Br[14C]AcNEtS-Rif with enzyme at a ratio of 1.4:1 and a concentration of 63 nM was found to proceed with a half life of 1 h at 37 degrees C. The enzyme could be protected from reaction with BrAcNEtS-Rif by either rifampicin or the hybrid [poly(dT)]-[r(Ap)5a]. The modification of holoenzyme by Br[14C]AcNEtS-Rif in the presence of p-hydroxymercuribenzene sulfonic acid (pOH-HgBzSO3H) or 4 M LiCl occurred with faster kinetics and led to a higher degree of substitution. Reaction of Br[14C]AcNEtS-Rif with RNA polymerase core enzyme caused predominant substitution of subunit beta. In the case of RNA polymerase holenzyme the radioactive substituents were evenly distributed between subunits beta and sigma. Apparently the topology of the rifamycin binding site of holoenzyme, similarly to core enzyme, precludes attacks of nucleophilic functions from beta' and alpha, but it allows nucleophilic functions from subunits beta and sigma to react with equal probability on BrAcNEtS-Rif. In the presence of a 20-fold excess of pOH-HgBzSO3H, the modification of holoenzyme was drastically altered. Virtually all substitution took place on subunit beta', very little on beta and none on subunits sigma and alpha.", "contents": "The modification of DNA-dependent RNA polymerase from Escherichia coli by an alkylating derivative of rifamycin SV. 3-(2-Bromo[1-14C]acetamidoethyl)-thio-rifamycin SV, abbreviated BrAcNEtS-Rif, and alkylating derivative of rifamycin SV was synthesized. A four-fold excess of BrAcNEtS-Rif inhibited the enzymic activity of RNA polymerase from Escherichia coli to 97%. Incubation of RNA polymerase with Br[14C]AcNEtS-Rif led to covalent substitution. The reaction of Br[14C]AcNEtS-Rif with enzyme at a ratio of 1.4:1 and a concentration of 63 nM was found to proceed with a half life of 1 h at 37 degrees C. The enzyme could be protected from reaction with BrAcNEtS-Rif by either rifampicin or the hybrid [poly(dT)]-[r(Ap)5a]. The modification of holoenzyme by Br[14C]AcNEtS-Rif in the presence of p-hydroxymercuribenzene sulfonic acid (pOH-HgBzSO3H) or 4 M LiCl occurred with faster kinetics and led to a higher degree of substitution. Reaction of Br[14C]AcNEtS-Rif with RNA polymerase core enzyme caused predominant substitution of subunit beta. In the case of RNA polymerase holenzyme the radioactive substituents were evenly distributed between subunits beta and sigma. Apparently the topology of the rifamycin binding site of holoenzyme, similarly to core enzyme, precludes attacks of nucleophilic functions from beta' and alpha, but it allows nucleophilic functions from subunits beta and sigma to react with equal probability on BrAcNEtS-Rif. In the presence of a 20-fold excess of pOH-HgBzSO3H, the modification of holoenzyme was drastically altered. Virtually all substitution took place on subunit beta', very little on beta and none on subunits sigma and alpha."}
{"id": "PMID:1100388", "title": "Role of ribosomal protein S1 in portein synthesis: effects of its addition to Bacillus stearothermophilus cell-free system.", "content": "Products of the f2 phage RNA-directed protein synthesizing systems in vitro, derived from Escherichia coli and Bacillus stearothermophilus, were analyzed. In contrast to other reports, it was found that B. stearothermophilus ribosomes synthesized phage coat protein when the high-speed (100 000 X g) supernatant (S100) and ribosomal wash (crude initiation factors) of homologous origin were used. Furthermore, a marked stimulation of coat protein synthesis was observed with B. stearothermophilus ribosomes when either S100 or ribosomal wash or both from E. coli cells were used instead of the respective homologous components. The principle responsible for this stimulation was identified as the 30-S ribosomal protein S1 present in S100 and ribosomal wash. Purified S1 from E. coli at roughly one-to-one molar ratio to ribosomes resulted in about 10-fold stimulation of the incorporation of [14C]valine when added to the B. stearothermophilus system. This stimulation was observed mainly in the synthesis of coat protein and replicase.", "contents": "Role of ribosomal protein S1 in portein synthesis: effects of its addition to Bacillus stearothermophilus cell-free system. Products of the f2 phage RNA-directed protein synthesizing systems in vitro, derived from Escherichia coli and Bacillus stearothermophilus, were analyzed. In contrast to other reports, it was found that B. stearothermophilus ribosomes synthesized phage coat protein when the high-speed (100 000 X g) supernatant (S100) and ribosomal wash (crude initiation factors) of homologous origin were used. Furthermore, a marked stimulation of coat protein synthesis was observed with B. stearothermophilus ribosomes when either S100 or ribosomal wash or both from E. coli cells were used instead of the respective homologous components. The principle responsible for this stimulation was identified as the 30-S ribosomal protein S1 present in S100 and ribosomal wash. Purified S1 from E. coli at roughly one-to-one molar ratio to ribosomes resulted in about 10-fold stimulation of the incorporation of [14C]valine when added to the B. stearothermophilus system. This stimulation was observed mainly in the synthesis of coat protein and replicase."}
{"id": "PMID:1100389", "title": "Nucleotide sequences of Escherichia coli 16-S RNA associated with ribosomal proteins S7, S9, S10, S14 and S19.", "content": "1. A ribonucleoprotein fragment containing proteins S7, S9, S10, S14, and S19 was isolated in high yield from Escherichia coli 30-S ribosomal sub-particles. The same fragment was obtained whether ribosomes from E. coli strain A19 or MRE 600 were used, despite the fact that protein S7 differs widely between the two strains. RNA was extracted from this fragment and fractionated on gels containing 7 M urea, to reveal \"hidden breaks\". A well-defined and reporducible pattern of RNA fragments was obtained, with the main components being approximately 300, 240, 130, 115 and 75 nucleotides in length, respectively. The pattern of RNA fragments obtained was also independent of the strain of E. coli used. 2. Two-dimensional fingerprints were made from ribonuclease T1 hydrolysates of these RNA fragments, labelled with 32P, and the oligonucleotides were further analysed by digestion with either ribonuclease A or U2. The data obtained were fitted in detail to the new 16-S RNA sequence map of Ehresmann et al. (1975). Again no significant differences were observed between the RNA from E. coli A19 or MRE 600. The RNA sequences found lay in the region O'-D-E'-K-P-P'-E-A of the 16-S RNA, with a clear excision of several nucleotides in section E'-K. The total sequence length covered was approximately 430 nucleotides.", "contents": "Nucleotide sequences of Escherichia coli 16-S RNA associated with ribosomal proteins S7, S9, S10, S14 and S19. 1. A ribonucleoprotein fragment containing proteins S7, S9, S10, S14, and S19 was isolated in high yield from Escherichia coli 30-S ribosomal sub-particles. The same fragment was obtained whether ribosomes from E. coli strain A19 or MRE 600 were used, despite the fact that protein S7 differs widely between the two strains. RNA was extracted from this fragment and fractionated on gels containing 7 M urea, to reveal \"hidden breaks\". A well-defined and reporducible pattern of RNA fragments was obtained, with the main components being approximately 300, 240, 130, 115 and 75 nucleotides in length, respectively. The pattern of RNA fragments obtained was also independent of the strain of E. coli used. 2. Two-dimensional fingerprints were made from ribonuclease T1 hydrolysates of these RNA fragments, labelled with 32P, and the oligonucleotides were further analysed by digestion with either ribonuclease A or U2. The data obtained were fitted in detail to the new 16-S RNA sequence map of Ehresmann et al. (1975). Again no significant differences were observed between the RNA from E. coli A19 or MRE 600. The RNA sequences found lay in the region O'-D-E'-K-P-P'-E-A of the 16-S RNA, with a clear excision of several nucleotides in section E'-K. The total sequence length covered was approximately 430 nucleotides."}
{"id": "PMID:1100390", "title": "Cell-wall lipopolysaccharide from Escherichia coli B.", "content": "The lipopolysaccharide of Escherichia coli BB and a number of R-phage selected (e.g. T3, T4) cell-wall-defective mutants were analyzed. From their lipopolysaccharides the respective core oligosaccharides were obtained. Following dephosphorylation, the oligosaccharides were methylated and analyzed by gas chromatography/mass spectrometry. This revealed the sugar sequence in the hexose-heptose region of the core. The linkage of heptose (Hep) to 2-keto-3-deoxyoctonate (KDO) was established as ... Hep 1,5 leads to KDO ... by methylation analysis. The substituted derivative of KDO was identified by gas chromatography and mass spectrometry. The KDO region contains three KDO units. Its structure was elaborated by (a) selective removal and identification of 7-phosphoryl ethanolamine-KDO (KDO-PN), (b) periodate oxidation and thiobarbituric acid reaction in conjunction with mild hydrolysis, (c) a modified methylation analysis. Phosphate substitution of E. coli BB core was studied by beta-elimination and using the information obtained with KDO-PN. The structures of the cell wall lipopolysaccharides from E. coli BB and cell-wall-defective mutants are given.", "contents": "Cell-wall lipopolysaccharide from Escherichia coli B. The lipopolysaccharide of Escherichia coli BB and a number of R-phage selected (e.g. T3, T4) cell-wall-defective mutants were analyzed. From their lipopolysaccharides the respective core oligosaccharides were obtained. Following dephosphorylation, the oligosaccharides were methylated and analyzed by gas chromatography/mass spectrometry. This revealed the sugar sequence in the hexose-heptose region of the core. The linkage of heptose (Hep) to 2-keto-3-deoxyoctonate (KDO) was established as ... Hep 1,5 leads to KDO ... by methylation analysis. The substituted derivative of KDO was identified by gas chromatography and mass spectrometry. The KDO region contains three KDO units. Its structure was elaborated by (a) selective removal and identification of 7-phosphoryl ethanolamine-KDO (KDO-PN), (b) periodate oxidation and thiobarbituric acid reaction in conjunction with mild hydrolysis, (c) a modified methylation analysis. Phosphate substitution of E. coli BB core was studied by beta-elimination and using the information obtained with KDO-PN. The structures of the cell wall lipopolysaccharides from E. coli BB and cell-wall-defective mutants are given."}
{"id": "PMID:1100391", "title": "Malonyl and palmityl transferase-less mutants of the yeast fatty-acid-synthetase complex.", "content": "146 independently isolated mutants of the fatty acid synthetase gene locus fas 1 were subdivided into six different complementation groups. Three of these groups, Va, Vb and Vd, have not been described before. The mutant fatty acid synthetases isolated from representatives of complementation group Vb were specifically deficient in two component enzymes at the same time, the malonyl and palmityl transferases. Among more than 180 fas 1 and fas 2 mutants systematically screened for malonyl and palmityl transferase activities no mutant was found affected in only one of these two fatty acid synthetase component enzymes. From this it is concluded that both transfer reactions are catalyzed by the same enzyme. In any malonyl transferase-less fatty acid synthetase, neither of the two known malonyl binding sites, i.e. enzyme-bound pantetheine and the non-thiol binding site, accepts malonate. This indicates that malonate is transferred to both groups by the same enzyme. So far, no acetyl transferase-less fas mutants have been characterized. On the other hand, the mutants of two fas 1 complementation groups, Va and Vd, though negative in overall fatty acid synthetase activity had no deficiency in any of the known component enzymes which can be tested in vitro. A possible interrelationship between both findings is discussed.", "contents": "Malonyl and palmityl transferase-less mutants of the yeast fatty-acid-synthetase complex. 146 independently isolated mutants of the fatty acid synthetase gene locus fas 1 were subdivided into six different complementation groups. Three of these groups, Va, Vb and Vd, have not been described before. The mutant fatty acid synthetases isolated from representatives of complementation group Vb were specifically deficient in two component enzymes at the same time, the malonyl and palmityl transferases. Among more than 180 fas 1 and fas 2 mutants systematically screened for malonyl and palmityl transferase activities no mutant was found affected in only one of these two fatty acid synthetase component enzymes. From this it is concluded that both transfer reactions are catalyzed by the same enzyme. In any malonyl transferase-less fatty acid synthetase, neither of the two known malonyl binding sites, i.e. enzyme-bound pantetheine and the non-thiol binding site, accepts malonate. This indicates that malonate is transferred to both groups by the same enzyme. So far, no acetyl transferase-less fas mutants have been characterized. On the other hand, the mutants of two fas 1 complementation groups, Va and Vd, though negative in overall fatty acid synthetase activity had no deficiency in any of the known component enzymes which can be tested in vitro. A possible interrelationship between both findings is discussed."}
{"id": "PMID:1100392", "title": "Histidyl-tRNA synthetase from Salmonella typhimurium: specificity in the binding of histidine analogues.", "content": "The topography of the active site of histidyl-tRNA synthetase has been investigated by determining Ki values for a variety of structural analogues of histidine, using the ATP-PPi exchange and tRNA aminoacylation reactions. Using these kinetic constants it has been possible to have a measure of the relative binding affinity of the enzyme for the histidine analogues. The following conclusions have been drawn: (a) the enzyme is stereospecific in the formation of aminoacyl-tRNA complexes, since the D-isomer of histidine does not influence the two reactions; (b) the carboxyl group is not required for binding; (c) bulky derivatives of the carboxyl group prevent the molecules from binding to the enzyme; (d) the amino group permits a good binding affinity; (e) the length of the ring side chain plays a very important role as point of attachment to the enzyme; (f) the kinds of heteroatoms on the ring determine the inhibitory properties of the analogues.", "contents": "Histidyl-tRNA synthetase from Salmonella typhimurium: specificity in the binding of histidine analogues. The topography of the active site of histidyl-tRNA synthetase has been investigated by determining Ki values for a variety of structural analogues of histidine, using the ATP-PPi exchange and tRNA aminoacylation reactions. Using these kinetic constants it has been possible to have a measure of the relative binding affinity of the enzyme for the histidine analogues. The following conclusions have been drawn: (a) the enzyme is stereospecific in the formation of aminoacyl-tRNA complexes, since the D-isomer of histidine does not influence the two reactions; (b) the carboxyl group is not required for binding; (c) bulky derivatives of the carboxyl group prevent the molecules from binding to the enzyme; (d) the amino group permits a good binding affinity; (e) the length of the ring side chain plays a very important role as point of attachment to the enzyme; (f) the kinds of heteroatoms on the ring determine the inhibitory properties of the analogues."}
{"id": "PMID:1100393", "title": "Biosynthesis of sterols by a yeast homogenate. Incorporation of mevalonic acid into cholesta-5,7,24-trien-3beta-ol and 5alpha-cholesta-7,24-dien-3beta-ol.", "content": "Incubation of (3RS,2R)-[2-14C,2-3H]mevalonic acid and (3RS, 2S)-[2-14C,2-3H]mevalonic acid with mechanically disrupted yeast cells resulted in C27-metabolites. Two (14C5, 3H4)-metabolites, cholesta-5,7,24-trien-3beta-ol and 5 alpha-cholesta-7,24-dien-3beta-ol, were isolated and characterized. The impairment of the 24-methyl transferase system was confirmed by the lack of incorporation of 14C into the sterol fraction on incubation of S-adenosyl-L-[methyl-14C]methionine with the yeast homogenate. The results indicate that interference with the (C-24)-alkylating system did not prevent the transformation of lanosterol to the cholesta-5,7,24-trien-3beta-ol and to 5 alpha-cholesta-7,24-dien-3beta-ol. It can therefore be inferred that transformations of the nucleus and of the side chain can function independently. However our results do not provide a definition of the actual sequence of the metabolic events between lanosterol and ergosterol.", "contents": "Biosynthesis of sterols by a yeast homogenate. Incorporation of mevalonic acid into cholesta-5,7,24-trien-3beta-ol and 5alpha-cholesta-7,24-dien-3beta-ol. Incubation of (3RS,2R)-[2-14C,2-3H]mevalonic acid and (3RS, 2S)-[2-14C,2-3H]mevalonic acid with mechanically disrupted yeast cells resulted in C27-metabolites. Two (14C5, 3H4)-metabolites, cholesta-5,7,24-trien-3beta-ol and 5 alpha-cholesta-7,24-dien-3beta-ol, were isolated and characterized. The impairment of the 24-methyl transferase system was confirmed by the lack of incorporation of 14C into the sterol fraction on incubation of S-adenosyl-L-[methyl-14C]methionine with the yeast homogenate. The results indicate that interference with the (C-24)-alkylating system did not prevent the transformation of lanosterol to the cholesta-5,7,24-trien-3beta-ol and to 5 alpha-cholesta-7,24-dien-3beta-ol. It can therefore be inferred that transformations of the nucleus and of the side chain can function independently. However our results do not provide a definition of the actual sequence of the metabolic events between lanosterol and ergosterol."}
{"id": "PMID:1100394", "title": "Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes.", "content": "After digestion of protein S4 with trypsin, all 32 tryptic peptides were isolated. Their amino acid compositions were analyzed and the sequence of the amino acids within the tryptic peptides was determined by means of a solid-phase peptide sequenator and by exopeptidases. Alignment of the tryptic peptides was established by analyzing and partially sequencing peptides isolated after digestion of the S4 protein with chymotrypsin, thermolysin and a glutamic-acid-specific protease. Further information about the alignment of peptides came from treatment of S4 with CNBr and with a lysine-modifying reagent.", "contents": "Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes. After digestion of protein S4 with trypsin, all 32 tryptic peptides were isolated. Their amino acid compositions were analyzed and the sequence of the amino acids within the tryptic peptides was determined by means of a solid-phase peptide sequenator and by exopeptidases. Alignment of the tryptic peptides was established by analyzing and partially sequencing peptides isolated after digestion of the S4 protein with chymotrypsin, thermolysin and a glutamic-acid-specific protease. Further information about the alignment of peptides came from treatment of S4 with CNBr and with a lysine-modifying reagent."}
{"id": "PMID:1100395", "title": "The primary structure of tRNAIIArg from brewers' yeast. 1. Complete digestions with pancreatic and T1 ribonucleases.", "content": "tRNAIIArg purified from bulk brewers' yeast tRNA by countercurrent distribution followed by two column-chromatographic steps was completely digested with pancreatic and T1 ribonucleases. Isolations of the products have been carried out either by column chromatography or by high-voltage electrophoresis. Analyses of the isolated nucleotides and olignoucleotides were in good agreement and indicate that this tRNA is composed of 76 nucltotide residues including 13 minor nucleotides. Overlaps resulting from the end-products of the two complementary digests led to a sequence of 25 residues. The primary structure of tRNAIIArg has been determined after partial digestion with T1 ribonuclease as described in the following paper.", "contents": "The primary structure of tRNAIIArg from brewers' yeast. 1. Complete digestions with pancreatic and T1 ribonucleases. tRNAIIArg purified from bulk brewers' yeast tRNA by countercurrent distribution followed by two column-chromatographic steps was completely digested with pancreatic and T1 ribonucleases. Isolations of the products have been carried out either by column chromatography or by high-voltage electrophoresis. Analyses of the isolated nucleotides and olignoucleotides were in good agreement and indicate that this tRNA is composed of 76 nucltotide residues including 13 minor nucleotides. Overlaps resulting from the end-products of the two complementary digests led to a sequence of 25 residues. The primary structure of tRNAIIArg has been determined after partial digestion with T1 ribonuclease as described in the following paper."}
{"id": "PMID:1100397", "title": "Isolation and properties of a thymidylate-less mutant in Saccharomyces cerevisiae.", "content": "A mutant, tmp3, has been isolated in Saccharomyces cerevisiae. Genetic and physiological analysis show that a single mendelian gene controls the multiple requirements for thymidylate, methionine, adenine and histidine and a neutral cytoplasmic petite character. Crude extracts of this mutant present a 60% decrease of serine transhydroxymethylase specific activity as compared to a wild-type strain.", "contents": "Isolation and properties of a thymidylate-less mutant in Saccharomyces cerevisiae. A mutant, tmp3, has been isolated in Saccharomyces cerevisiae. Genetic and physiological analysis show that a single mendelian gene controls the multiple requirements for thymidylate, methionine, adenine and histidine and a neutral cytoplasmic petite character. Crude extracts of this mutant present a 60% decrease of serine transhydroxymethylase specific activity as compared to a wild-type strain."}
{"id": "PMID:1100398", "title": "Purification and properties of phosphoglucomutase from Fleischmann's yeast.", "content": "1. A procedure has been described for the purification of the major isozyme of yeast phosphoglucomutase of highest known specific activity. 2. The native enzyme has a molecular weight of about 65400 and was found to be homogeneous as judged by sucrose density gradient centrifugation, gel filtration, electrophoresis on acrylamide gel and ultracentrifugal analysis. In the presence of denaturing agents such as guanidine hydrochloride or sodium dodecyl sulfate, the enzyme dissociated into 32000-molecular-weight subunits. 3. As isolated, the enzyme has one mole of phosphate bound per mole of enzyme. Preparations incubated with 1.0 mM EDTA in 10 mM citrate buffer, pH 5.5 and dialysed against 10 mM metal-free citrate buffer, pH 5.5, contain no intrinsically bound Zn2+ and were enzymically inactive but fully active in the presence of 5 mM Mg2+ and 84% as active with 0.5 mM Zn2+. Simultaneous presence of both ions at these concentrations did not enhance activity. Enzyme was completely and irreversibly inactivated by preincubation with Be2+. Inactive enzyme had one mole of Be2+ bound per mole of enzyme. 4. Enzyme exhibited \"ping-pong\" kinetics rather than \"random sequential\". Km values for glucose 1-phosphate and for glucose 1,6-bisphosphate were calculated to be 2.34 times 10(-5) M and 2.24 times 10(-6) M, respectively. Rate of enzyme phosphate turnover was studied with rapid-mixing technique. The rates of 32P release from 32P-labeled enzyme and its appearance as glucose 6-[32P]phosphate were comparable and remained unaffected by addition of glucose 1,6-bisphosphate.", "contents": "Purification and properties of phosphoglucomutase from Fleischmann's yeast. 1. A procedure has been described for the purification of the major isozyme of yeast phosphoglucomutase of highest known specific activity. 2. The native enzyme has a molecular weight of about 65400 and was found to be homogeneous as judged by sucrose density gradient centrifugation, gel filtration, electrophoresis on acrylamide gel and ultracentrifugal analysis. In the presence of denaturing agents such as guanidine hydrochloride or sodium dodecyl sulfate, the enzyme dissociated into 32000-molecular-weight subunits. 3. As isolated, the enzyme has one mole of phosphate bound per mole of enzyme. Preparations incubated with 1.0 mM EDTA in 10 mM citrate buffer, pH 5.5 and dialysed against 10 mM metal-free citrate buffer, pH 5.5, contain no intrinsically bound Zn2+ and were enzymically inactive but fully active in the presence of 5 mM Mg2+ and 84% as active with 0.5 mM Zn2+. Simultaneous presence of both ions at these concentrations did not enhance activity. Enzyme was completely and irreversibly inactivated by preincubation with Be2+. Inactive enzyme had one mole of Be2+ bound per mole of enzyme. 4. Enzyme exhibited \"ping-pong\" kinetics rather than \"random sequential\". Km values for glucose 1-phosphate and for glucose 1,6-bisphosphate were calculated to be 2.34 times 10(-5) M and 2.24 times 10(-6) M, respectively. Rate of enzyme phosphate turnover was studied with rapid-mixing technique. The rates of 32P release from 32P-labeled enzyme and its appearance as glucose 6-[32P]phosphate were comparable and remained unaffected by addition of glucose 1,6-bisphosphate."}
{"id": "PMID:1100399", "title": "The thermodynamics and kinetics of conformational changes in 5-S RNA from Escherichia coli.", "content": "Solutions of native, renatured and denatured 5-S RNA from Escherichia coli were each found to revert to a mixture of the denatured and renatured forms when incubated at elevated temperatures in the absence of divalent cations. The equilibrium and rate constants for the renaturation and denaturation reactions were studied as a function of temperature and concentration of added NaCl. The enthalpy and entropy of denaturation and the activation energies were determined. The activation energies for the renaturation reaction were found to be almost independent of the salt concentration, whereas the other parameters increased with the salt concentration. The results are interpreted in terms of a partially unfolded intermediate whose concentration is suppressed by the addition of salt.", "contents": "The thermodynamics and kinetics of conformational changes in 5-S RNA from Escherichia coli. Solutions of native, renatured and denatured 5-S RNA from Escherichia coli were each found to revert to a mixture of the denatured and renatured forms when incubated at elevated temperatures in the absence of divalent cations. The equilibrium and rate constants for the renaturation and denaturation reactions were studied as a function of temperature and concentration of added NaCl. The enthalpy and entropy of denaturation and the activation energies were determined. The activation energies for the renaturation reaction were found to be almost independent of the salt concentration, whereas the other parameters increased with the salt concentration. The results are interpreted in terms of a partially unfolded intermediate whose concentration is suppressed by the addition of salt."}
{"id": "PMID:1100401", "title": "An inhibitory effect of thymidine on its own conversion to nucleotide in Escherichia coli.", "content": "The cellular levels of thymidine nucleotide pool in thymine-requiring mutants of Escherichia coli were followed. The pool levels of dTDP, dTTP and compound-X reached maximal values in much lower concentration of thymidine than those in thymine. In higher concentrations of thymidine, an inhibitory effect on its own conversion to nucleotide was observed. The inhibited step was suggested to be the conversion of dTMP to dTDP.", "contents": "An inhibitory effect of thymidine on its own conversion to nucleotide in Escherichia coli. The cellular levels of thymidine nucleotide pool in thymine-requiring mutants of Escherichia coli were followed. The pool levels of dTDP, dTTP and compound-X reached maximal values in much lower concentration of thymidine than those in thymine. In higher concentrations of thymidine, an inhibitory effect on its own conversion to nucleotide was observed. The inhibited step was suggested to be the conversion of dTMP to dTDP."}
{"id": "PMID:1100402", "title": "The regulation of arginine biosynthesis in Saccharomyces cerevisiae. The specificity of argR- mutations and the general control of amino-acid biosynthesis.", "content": "The regulation of arginine biosynthetic enzymes in yeast is subjected to a double control. One level of arginine enzyme synthesis is under the control of an apo-repressor, called ARGR. ARGR molecules control specifically the arginine pathway. A second level of control of arginine biosynthesis has been disclosed. It also controls tryptophan, histidine, lysine, isoleucine-valine and probably many more biosyntheses. The general mechanism is turned on in leaky mutants in any of the amino acid pathways mentioned above.", "contents": "The regulation of arginine biosynthesis in Saccharomyces cerevisiae. The specificity of argR- mutations and the general control of amino-acid biosynthesis. The regulation of arginine biosynthetic enzymes in yeast is subjected to a double control. One level of arginine enzyme synthesis is under the control of an apo-repressor, called ARGR. ARGR molecules control specifically the arginine pathway. A second level of control of arginine biosynthesis has been disclosed. It also controls tryptophan, histidine, lysine, isoleucine-valine and probably many more biosyntheses. The general mechanism is turned on in leaky mutants in any of the amino acid pathways mentioned above."}
{"id": "PMID:1100403", "title": "Pools of ribosomal proteins in Escherichia coli. Studies on the exchange of proteins between pools and ribosomes.", "content": "A labelling technique in vivo has been introduced which allows the tritiation of cell components with high specific activity during growth in rich medium. By this technique the pool size of each protein can be measured directly in the supernatant from centrifugation at 150000 times g. A measurable pool was found for the proteins S1, S2, S10, L1, L4, L7, L8/9, L10, L12, L21, and L25. Experiments on migration of ribosomal proteins from the supernatant to ribosomes (i.e. association) and vice versa (dissociation) demonstrate a remarkable constancy in the composition of the ribosome. There is no significant difference between ribosomes engaged or not engaged in poly-(Phe) synthesis.", "contents": "Pools of ribosomal proteins in Escherichia coli. Studies on the exchange of proteins between pools and ribosomes. A labelling technique in vivo has been introduced which allows the tritiation of cell components with high specific activity during growth in rich medium. By this technique the pool size of each protein can be measured directly in the supernatant from centrifugation at 150000 times g. A measurable pool was found for the proteins S1, S2, S10, L1, L4, L7, L8/9, L10, L12, L21, and L25. Experiments on migration of ribosomal proteins from the supernatant to ribosomes (i.e. association) and vice versa (dissociation) demonstrate a remarkable constancy in the composition of the ribosome. There is no significant difference between ribosomes engaged or not engaged in poly-(Phe) synthesis."}
{"id": "PMID:1100404", "title": "Fluorescent labelling of Escherichia coli ribosomal sulfhydryl groups.", "content": "The reactivity of the sulfhydryl groups of Escherichia coli ribosome has been investigated using a fluorescent label. Under denaturing conditions, all the --SH groups can be titrated. In the native form of the ribosome, six and less than one labels can be respectively conjugated to the 30-S and 50-S subparticles without loss of activity for poly(U)-dependent polyphenylalanine synthesis. The most reactive thiol groups belong to proteins S1, S12, S18, S21. The binding of mRNA plus tRNA to the 70-S ribosomes affects the spectroscopic properties of the labels showing that conformational changes are induced by these interactions. Furthermore, the treatment of these complexes by the labelling agent demonstrates that the --SH group belonging to protein S1 is partially protected whereas other thiol groups located on the 50-S subparticle become reactive.", "contents": "Fluorescent labelling of Escherichia coli ribosomal sulfhydryl groups. The reactivity of the sulfhydryl groups of Escherichia coli ribosome has been investigated using a fluorescent label. Under denaturing conditions, all the --SH groups can be titrated. In the native form of the ribosome, six and less than one labels can be respectively conjugated to the 30-S and 50-S subparticles without loss of activity for poly(U)-dependent polyphenylalanine synthesis. The most reactive thiol groups belong to proteins S1, S12, S18, S21. The binding of mRNA plus tRNA to the 70-S ribosomes affects the spectroscopic properties of the labels showing that conformational changes are induced by these interactions. Furthermore, the treatment of these complexes by the labelling agent demonstrates that the --SH group belonging to protein S1 is partially protected whereas other thiol groups located on the 50-S subparticle become reactive."}
{"id": "PMID:1100405", "title": "Amino-acid composition of the covalent rigid-layer lipoprotein in cell walls of Proteus mirabilis.", "content": "Cell walls of Proteus mirabilis in the stationary phase of growth contain a lipoprotein in covalent linkage to peptidoglycan and probably also in free form in the outer membrane. The protein moiety of this lipoprotein is composed of about 50 amino acids and has an approximate molecular weight of 5500. The Proteus lipoprotein has glycine and phenylalanine as specific components which are not present in lipoproteins of other enteric bacteria. Treatment of the peptidoglycan-lipoprotein complex of Proteus with trypsin leaves lysine as the only lipoprotein amino acid attached to the peptidoglycan. This suggests that in P. mirabilis, as in Escherichia coli, the lipoprotein is linked to the peptidoglycan by its C-terminal lysine residue.", "contents": "Amino-acid composition of the covalent rigid-layer lipoprotein in cell walls of Proteus mirabilis. Cell walls of Proteus mirabilis in the stationary phase of growth contain a lipoprotein in covalent linkage to peptidoglycan and probably also in free form in the outer membrane. The protein moiety of this lipoprotein is composed of about 50 amino acids and has an approximate molecular weight of 5500. The Proteus lipoprotein has glycine and phenylalanine as specific components which are not present in lipoproteins of other enteric bacteria. Treatment of the peptidoglycan-lipoprotein complex of Proteus with trypsin leaves lysine as the only lipoprotein amino acid attached to the peptidoglycan. This suggests that in P. mirabilis, as in Escherichia coli, the lipoprotein is linked to the peptidoglycan by its C-terminal lysine residue."}
{"id": "PMID:1100406", "title": "The interaction of fusidic acid with peptidyl-transfer-ribonucleic-acid - ribosome complexes.", "content": "The inhibitory action of fusidic acid on peptide-chain elongation was studied with systems in vitro directed by either polyuridylic acid or endogenous messenger (Escherichia coli polysomes washed with 1 M NH4Cl) or R17 RNA, and supplemented with either crude or purified elongation factors. In all cases strong inhibition of synthesis required high concentrations of the antibiotic (approx. 1 mM), while a similar inhibition of the EF-G-plus-ribosome-dependent GTP hydrolysis required between 10 and 100 times less antibiotic. Since most of the GTP hydrolysis observed was presumably due to free ribosomes (without aminoacyl-tRNA or peptidyl-tRNA), fusidic acid seemed to interact far more easily with these ribosomes than with ribosomes engaged in peptide-chain elongation. The role of the GDP-EF-G-ribosome-fusidic acid complex in the inhibition of polypeptide synthesis was assessed by measuring formation of this complex on polysomes engaged in peptide-chain elongation. Using purified elongation factors the complex formed on only 25-35% of ribosomes, as measured either by retention of [3H]GDP or by hydrolysis of [3H, gamma-32P]GTP. In contrast, with crude factors (S 100 extract) it formed on more than 70% of ribosomes. The results are compatible with the postulated role of the complex in polypeptide synthesis inhibition (blockade of the ribosomal acceptor site and subsequent inhibition of aminoacyl-tRNA binding) and indicate that formation of the complex takes place by overriding the control that prevents interaction of EF-G when the donor site is occupied by peptidyl-tRNA. In the polyuridylic-acid-directed system for synthesis of oligophenylalanine the antibiotic inhibits every round of peptide elongation, including dipeptide formation, to roughly the same extent.", "contents": "The interaction of fusidic acid with peptidyl-transfer-ribonucleic-acid - ribosome complexes. The inhibitory action of fusidic acid on peptide-chain elongation was studied with systems in vitro directed by either polyuridylic acid or endogenous messenger (Escherichia coli polysomes washed with 1 M NH4Cl) or R17 RNA, and supplemented with either crude or purified elongation factors. In all cases strong inhibition of synthesis required high concentrations of the antibiotic (approx. 1 mM), while a similar inhibition of the EF-G-plus-ribosome-dependent GTP hydrolysis required between 10 and 100 times less antibiotic. Since most of the GTP hydrolysis observed was presumably due to free ribosomes (without aminoacyl-tRNA or peptidyl-tRNA), fusidic acid seemed to interact far more easily with these ribosomes than with ribosomes engaged in peptide-chain elongation. The role of the GDP-EF-G-ribosome-fusidic acid complex in the inhibition of polypeptide synthesis was assessed by measuring formation of this complex on polysomes engaged in peptide-chain elongation. Using purified elongation factors the complex formed on only 25-35% of ribosomes, as measured either by retention of [3H]GDP or by hydrolysis of [3H, gamma-32P]GTP. In contrast, with crude factors (S 100 extract) it formed on more than 70% of ribosomes. The results are compatible with the postulated role of the complex in polypeptide synthesis inhibition (blockade of the ribosomal acceptor site and subsequent inhibition of aminoacyl-tRNA binding) and indicate that formation of the complex takes place by overriding the control that prevents interaction of EF-G when the donor site is occupied by peptidyl-tRNA. In the polyuridylic-acid-directed system for synthesis of oligophenylalanine the antibiotic inhibits every round of peptide elongation, including dipeptide formation, to roughly the same extent."}
{"id": "PMID:1100407", "title": "The joining of the 30-S initiation complex with the 50-S subunit, the main target for thiostrepton.", "content": "The study undertaken in this paper on the mode of action of thiostrepton provides data which permit a more precise localization of the main target of thiostrepton. There is severe impairment of the joining of the 50-S subunit, probably carrying thiostrepton, with either the 30-S subunit or the 30-S initiation complex. The degree of impairment of this coupling is temperature dependent, being almost completely inhibited at 0 degrees C, whereas at 37 degrees C the effect is much less marked, provided that natural messenger RNA is present. The inhibition of initiation by thiostrepton is more severe in the presence of IF-1, a factor, which similar to thiostrepton, is able to shift the dynamic equilibrium of 70-S in equilibrium 50-S + 30-S more towards dissociation. By means of 14C-labeled IF-2 it is demonstrated that the binding of IF-2 into the 70-S initiation complex is prevented by thiostrepton, which seems to be the main cause for non-coupling.", "contents": "The joining of the 30-S initiation complex with the 50-S subunit, the main target for thiostrepton. The study undertaken in this paper on the mode of action of thiostrepton provides data which permit a more precise localization of the main target of thiostrepton. There is severe impairment of the joining of the 50-S subunit, probably carrying thiostrepton, with either the 30-S subunit or the 30-S initiation complex. The degree of impairment of this coupling is temperature dependent, being almost completely inhibited at 0 degrees C, whereas at 37 degrees C the effect is much less marked, provided that natural messenger RNA is present. The inhibition of initiation by thiostrepton is more severe in the presence of IF-1, a factor, which similar to thiostrepton, is able to shift the dynamic equilibrium of 70-S in equilibrium 50-S + 30-S more towards dissociation. By means of 14C-labeled IF-2 it is demonstrated that the binding of IF-2 into the 70-S initiation complex is prevented by thiostrepton, which seems to be the main cause for non-coupling."}
{"id": "PMID:1100408", "title": "Preferential digestion of (A plus T)-rich stretches of yeast mitochondrial DNA in isolated mitochondria.", "content": "Yeast mitochondrial DNA labelled in vitro by incubation of isolated mitochondria with DNA precursors exhibits skewed profiles on isopycnic CsCl gradients. The skew is not due to nuclear DNA nor to single-stranded mitochondrial DNA in the product labelled in vitro. Simultaneous labelling with [3H]dTTP and [14C]dGTP in vitro indicates a gradient of base composition in the DNA labelled in vitro. Thus, selective degradation of mitochondrial DNA occurs during incubation, converting large molecules having the mean density of mitochondrial DNA into smaller molecules of higher mean density and with higher G:T ratio. Similarly skewed distributions can also be produced by incubation of mitochondrial DNA labelled in vivo with the yeast mitochondrial fraction or with micrococcal endonuclease, an enzyme known to selectively hydrolyse (A plus T)-rich regions of DNA.", "contents": "Preferential digestion of (A plus T)-rich stretches of yeast mitochondrial DNA in isolated mitochondria. Yeast mitochondrial DNA labelled in vitro by incubation of isolated mitochondria with DNA precursors exhibits skewed profiles on isopycnic CsCl gradients. The skew is not due to nuclear DNA nor to single-stranded mitochondrial DNA in the product labelled in vitro. Simultaneous labelling with [3H]dTTP and [14C]dGTP in vitro indicates a gradient of base composition in the DNA labelled in vitro. Thus, selective degradation of mitochondrial DNA occurs during incubation, converting large molecules having the mean density of mitochondrial DNA into smaller molecules of higher mean density and with higher G:T ratio. Similarly skewed distributions can also be produced by incubation of mitochondrial DNA labelled in vivo with the yeast mitochondrial fraction or with micrococcal endonuclease, an enzyme known to selectively hydrolyse (A plus T)-rich regions of DNA."}
{"id": "PMID:1100409", "title": "Interaction of RNA polymerase from Escherichia coli with DNA. Analysis of T7 DNA early-promoter sites.", "content": "A method was devised for directing RNA polymerase on a single promoter site on T7 DNA. Initiation complexes were formed on each of the three main promoter sites using one dinucleotide plus one nucleoside triphosphate. The ternary initiation complexes are resistant to rifampicin action, to inhibition by (rI)n at 0 degrees C and are stable at high salt concentrations. A minimum of a trinucleotide is required to form a stable ternary complex. To determine which promoter site was selected by RNA polymerase during initiation, the (rI)n-resistant RNA was digested by RNAse III to generate three characteristic initiator RNA fragments, resolved by gel electrophoresis. The three major promoter sites could be selected individually by using different primer and substrate combinations ApC plus ATP selected promoter A3, CpG plus CTP selected A2 and CpC plus ATP specified preferentially A1. A number of primer-substrate combinations specified each site at low salt concentration but the substrate requirement became very stringent at high salt concentration, suggesting that the postulated local opening of the promoter site could be more or less extensive, depending on the ionic strength. The minimum opening observed at high salt concentration corresponded to the insertion of a leader trinucleotide sequence. The promoter region melted by RNA polymerase at low salt concentration was (G plus C)-rich and corresponded to about 9 to 11 base pairs. Sequences of the melting recognition regions were tentatively inferred from the results.", "contents": "Interaction of RNA polymerase from Escherichia coli with DNA. Analysis of T7 DNA early-promoter sites. A method was devised for directing RNA polymerase on a single promoter site on T7 DNA. Initiation complexes were formed on each of the three main promoter sites using one dinucleotide plus one nucleoside triphosphate. The ternary initiation complexes are resistant to rifampicin action, to inhibition by (rI)n at 0 degrees C and are stable at high salt concentrations. A minimum of a trinucleotide is required to form a stable ternary complex. To determine which promoter site was selected by RNA polymerase during initiation, the (rI)n-resistant RNA was digested by RNAse III to generate three characteristic initiator RNA fragments, resolved by gel electrophoresis. The three major promoter sites could be selected individually by using different primer and substrate combinations ApC plus ATP selected promoter A3, CpG plus CTP selected A2 and CpC plus ATP specified preferentially A1. A number of primer-substrate combinations specified each site at low salt concentration but the substrate requirement became very stringent at high salt concentration, suggesting that the postulated local opening of the promoter site could be more or less extensive, depending on the ionic strength. The minimum opening observed at high salt concentration corresponded to the insertion of a leader trinucleotide sequence. The promoter region melted by RNA polymerase at low salt concentration was (G plus C)-rich and corresponded to about 9 to 11 base pairs. Sequences of the melting recognition regions were tentatively inferred from the results."}
{"id": "PMID:1100419", "title": "Immunologic chimerism as evidence of bone marrow graft acceptance in an identical twin with acute lymphocytic leukemia.", "content": "Bone marrow from a well child was infused into her identical twin who had acute lymphocytic leukemia. In an attempt to provide an immunologic tag for use in twin transplantation, the donor twin was immunized to Keyhole limpet hemocyanin (KLH) and yellow fever virus prior to the marrow infusion. Subsequent immunological chimerism in the recipient twin gave evidence for graft acceptance.", "contents": "Immunologic chimerism as evidence of bone marrow graft acceptance in an identical twin with acute lymphocytic leukemia. Bone marrow from a well child was infused into her identical twin who had acute lymphocytic leukemia. In an attempt to provide an immunologic tag for use in twin transplantation, the donor twin was immunized to Keyhole limpet hemocyanin (KLH) and yellow fever virus prior to the marrow infusion. Subsequent immunological chimerism in the recipient twin gave evidence for graft acceptance."}
{"id": "PMID:1100420", "title": "Diffusion chamber culturing of haematopoietic cells: methodological investigations and improvement of the technique.", "content": "Mouse bone marrow cells were cultured to determine some basic characteristics of the diffusion chamber (DC) technique. The main findings were as follows: (i) Incorporated 3H-thymidine was conveniently measured after deposition of a cell sample on glass fibre discs followed by methanol washing. Varying the specific activity from approximately 2-approximately 20 Ci/mmole did not affect the relationship between DC cellularity and isotope incorporation. Isotope uptake was similar regardless of whether 3H-thymidine of high or low specific activity had been used. (ii) Higher cell yields were obtained when Millipore or Acropor filters were heat-sealed rather than glued to the plastic rings, when Millipore filters were moistened before DC filling, when we omitted gluing the closing plugs, and when we used an average pore size of 0.22 mum for the DC walls rather than one of 0.10 mum or 0.45 mum. Varying the ring thickness from 2.0-2.5 mm did not impair the cell growth, nor did removal of a softening agent from the plastic rings improve it. (iii) More cells were retrieved from DC carried by young rather than by older mice. Results were not influenced by sex or strain differences between the donor and the host or by the number of implants, whether single or double, within an individual host. (iv) Adding Ficoll to the pronase solution increased the yield of viable CFU-C. (V) Diffusion rate through the DC walls declined with increasing period of culturing, so that i.p. 3H-thymidine is not a flash label for 7-day cultures, for example. The great variability of 3H-thymidine diffusion into i.p. DC was markedly reduced by in vitro exposure to the unopened DC to the isotope. (vi) DC could be incubated in vitro in a medium devoid of protein for at least 6 hours without a fall in 3H-thymidine incorporation rate or CFU-C content, provided that pH was kept constant.", "contents": "Diffusion chamber culturing of haematopoietic cells: methodological investigations and improvement of the technique. Mouse bone marrow cells were cultured to determine some basic characteristics of the diffusion chamber (DC) technique. The main findings were as follows: (i) Incorporated 3H-thymidine was conveniently measured after deposition of a cell sample on glass fibre discs followed by methanol washing. Varying the specific activity from approximately 2-approximately 20 Ci/mmole did not affect the relationship between DC cellularity and isotope incorporation. Isotope uptake was similar regardless of whether 3H-thymidine of high or low specific activity had been used. (ii) Higher cell yields were obtained when Millipore or Acropor filters were heat-sealed rather than glued to the plastic rings, when Millipore filters were moistened before DC filling, when we omitted gluing the closing plugs, and when we used an average pore size of 0.22 mum for the DC walls rather than one of 0.10 mum or 0.45 mum. Varying the ring thickness from 2.0-2.5 mm did not impair the cell growth, nor did removal of a softening agent from the plastic rings improve it. (iii) More cells were retrieved from DC carried by young rather than by older mice. Results were not influenced by sex or strain differences between the donor and the host or by the number of implants, whether single or double, within an individual host. (iv) Adding Ficoll to the pronase solution increased the yield of viable CFU-C. (V) Diffusion rate through the DC walls declined with increasing period of culturing, so that i.p. 3H-thymidine is not a flash label for 7-day cultures, for example. The great variability of 3H-thymidine diffusion into i.p. DC was markedly reduced by in vitro exposure to the unopened DC to the isotope. (vi) DC could be incubated in vitro in a medium devoid of protein for at least 6 hours without a fall in 3H-thymidine incorporation rate or CFU-C content, provided that pH was kept constant."}
{"id": "PMID:1100421", "title": "Toxicologic and marrow transplantation studies in rhesus monkeys given dimethyl myleran.", "content": "The hematologic and pathologic effects of single doses of dimethyl myleran (DMM) were evaluated in rhesus monkeys with and without the infusion of marrow. DMM 3 to 5 mg/kg produced severe but reversible myelotoxicity and no non-marrow toxicity. All animals given DMM 10 mg/kg died of marrow failure which was consistently reversed by the infusion of cryopreserved autologous marrow. At higher doses of DMM the protective effect of autologous marrow was less consistent due to increased gastrointestinal toxicity. Allogeneic engraftment was not achieved following administration of 10 mg/kg of DMM. In two animals receiving 15 mg/kg, proliferating allogeneic donor cells were present in recipient marrow seven days after marrow infusion.", "contents": "Toxicologic and marrow transplantation studies in rhesus monkeys given dimethyl myleran. The hematologic and pathologic effects of single doses of dimethyl myleran (DMM) were evaluated in rhesus monkeys with and without the infusion of marrow. DMM 3 to 5 mg/kg produced severe but reversible myelotoxicity and no non-marrow toxicity. All animals given DMM 10 mg/kg died of marrow failure which was consistently reversed by the infusion of cryopreserved autologous marrow. At higher doses of DMM the protective effect of autologous marrow was less consistent due to increased gastrointestinal toxicity. Allogeneic engraftment was not achieved following administration of 10 mg/kg of DMM. In two animals receiving 15 mg/kg, proliferating allogeneic donor cells were present in recipient marrow seven days after marrow infusion."}
{"id": "PMID:1100422", "title": "Weakening of genetic resistance. I. The effect of injection of endotoxin, Freund's complete adjuvant and alloantiserum.", "content": "A parent to F1 transplantation combination was used to study the weakening effect of endotoxin, Freund's complete adjuvant and alloantiserum on genetic resistance. The relationship between time of treatment with endotoxin, Salmonella typhosa, and Freund's complete adjuvant, and their weakening effect was assessed by use of the spleen colony technique. CFU growth studies revealed that both endotoxin and alloantiserum were capable of weakening genetic resistance in the spleen but were unable to induce weakening of the resistance in the femoral marrow cavity. These results led us to the conclusion that the agents might not have a direct effect on the effector cells of the resistance. The weakening induced by endotoxin and alloantiserum seemed to be related to a certain immunological phenomenon in the spleen. In this phenomenon macrophages are likely to play a role since a number of agents capable of weakening resistance were known for their capacity to influence the mononuclear phagocytic system.", "contents": "Weakening of genetic resistance. I. The effect of injection of endotoxin, Freund's complete adjuvant and alloantiserum. A parent to F1 transplantation combination was used to study the weakening effect of endotoxin, Freund's complete adjuvant and alloantiserum on genetic resistance. The relationship between time of treatment with endotoxin, Salmonella typhosa, and Freund's complete adjuvant, and their weakening effect was assessed by use of the spleen colony technique. CFU growth studies revealed that both endotoxin and alloantiserum were capable of weakening genetic resistance in the spleen but were unable to induce weakening of the resistance in the femoral marrow cavity. These results led us to the conclusion that the agents might not have a direct effect on the effector cells of the resistance. The weakening induced by endotoxin and alloantiserum seemed to be related to a certain immunological phenomenon in the spleen. In this phenomenon macrophages are likely to play a role since a number of agents capable of weakening resistance were known for their capacity to influence the mononuclear phagocytic system."}
{"id": "PMID:1100426", "title": "The methylation of mercuric chloride by human intestinal bacteria.", "content": "Most strains of staphylococci, streptococci, yeasts and E. coli isolated from human faeces, could synthesize methylmercury compounds. In contrast, few strains of obligate anaerobes could do so. Up to 6 ng methylmercury/ml were formed in 44 h from 2 mug mercuric chloride.", "contents": "The methylation of mercuric chloride by human intestinal bacteria. Most strains of staphylococci, streptococci, yeasts and E. coli isolated from human faeces, could synthesize methylmercury compounds. In contrast, few strains of obligate anaerobes could do so. Up to 6 ng methylmercury/ml were formed in 44 h from 2 mug mercuric chloride."}
{"id": "PMID:1100427", "title": "Erythrocyte agglutinins in the blood of certain ascidians.", "content": "Plasma from Ciona intestinalis, Phallusia mamillata and Ascidia malaca possess hemagglutinin for a variety of erythrocytes. Results obtained by physical and chemical treatments suggest that hemagglutinin for Phallusia mamillata and Ascidia malaca may be a protein or a protein-like substance.", "contents": "Erythrocyte agglutinins in the blood of certain ascidians. Plasma from Ciona intestinalis, Phallusia mamillata and Ascidia malaca possess hemagglutinin for a variety of erythrocytes. Results obtained by physical and chemical treatments suggest that hemagglutinin for Phallusia mamillata and Ascidia malaca may be a protein or a protein-like substance."}
{"id": "PMID:1100435", "title": "Oxygen tension: dependent or independent variable in local control of blood flow?", "content": "We have looked at a fairly simple model of blood flow regulation at the microvascular level consisting of suffused microvascular preparations and isolated smooth muscles. When the O2 demand of the microvessel preparations was decreased by elevating suffusion solution PO2, changes in wall PO2 indicated that only the smallest microvessels could be controlled by a direct effect of oxygen. In vivo and in vitro studies of the oxygen sensitivity of smooth muscle indicated that even the smaller vessels were probably not directly controlled by oxygen availability during a free flow state, since measured perivascular PO2's did not fall to levels low enough to alter contractile performance of the vascular smooth muscle. Our data indicate that in any condition in which flow is interrupted for periods in excess of about 30 sec, one might anticipate vascular relaxation due to oxygen lack. It was judged resonable to extrapolate our findings to autoregulation of blood flow in resting skeletal muscle and to responses to modest exercise. Our data indicate that it is improbable that oxygen acts by a direct effect on the smooth muscle under these conditions. On the other hand, they suggest that a direct effect of oxygen might well be important in causing postocclusion hyperemia.", "contents": "Oxygen tension: dependent or independent variable in local control of blood flow? We have looked at a fairly simple model of blood flow regulation at the microvascular level consisting of suffused microvascular preparations and isolated smooth muscles. When the O2 demand of the microvessel preparations was decreased by elevating suffusion solution PO2, changes in wall PO2 indicated that only the smallest microvessels could be controlled by a direct effect of oxygen. In vivo and in vitro studies of the oxygen sensitivity of smooth muscle indicated that even the smaller vessels were probably not directly controlled by oxygen availability during a free flow state, since measured perivascular PO2's did not fall to levels low enough to alter contractile performance of the vascular smooth muscle. Our data indicate that in any condition in which flow is interrupted for periods in excess of about 30 sec, one might anticipate vascular relaxation due to oxygen lack. It was judged resonable to extrapolate our findings to autoregulation of blood flow in resting skeletal muscle and to responses to modest exercise. Our data indicate that it is improbable that oxygen acts by a direct effect on the smooth muscle under these conditions. On the other hand, they suggest that a direct effect of oxygen might well be important in causing postocclusion hyperemia."}
{"id": "PMID:1100437", "title": "Biochemical function of selenium and its relation to vitamin E.", "content": "Glutathione peroxidase (glutathione:H2O2 oxidoreductase, E.C. 1.11.1.9), isolated from ovine and bovine erythrocytes, has recently been shown to contain 4 selenium atoms per mole, an average of 1 Se per protein subunit of about 22,000 molecular weight. Selenium deficiency in the rat, chick and sheep causes dramatic decreases in the activity of this enzyme in the tissues, but certain sites such as liver are affected more than others. Decreases in glutathione peroxidase correlate with lesions caused by selenium deficiency and appear useful in diagnosing selenium deficiency. Glutathione peroxidase is an important enzyme in destroying H2O2 and organic hydroperoxides such as lipid hydroperoxides. It therefore guards against oxidative damage to the cell membranes and other oxidant-sensitive sites in the cell. While this selenium-dependent system destroys lipid hydroperoxides and other peroxides, vitamin E is believed to protect against oxidant damage to membranes by preventing the formation of lipid hydroperoxides. A scheme is proposed, based on oxidant damage and its prevention, which accounts for the interaction between selenium, vitamin E, unsaturated lipids, sulfur-containing amino acids, and cell damaging agents such as oxidant stressors and toxicants such as silver and tri-o-cresyl phosphate. The background for such a scheme is reviewed.", "contents": "Biochemical function of selenium and its relation to vitamin E. Glutathione peroxidase (glutathione:H2O2 oxidoreductase, E.C. 1.11.1.9), isolated from ovine and bovine erythrocytes, has recently been shown to contain 4 selenium atoms per mole, an average of 1 Se per protein subunit of about 22,000 molecular weight. Selenium deficiency in the rat, chick and sheep causes dramatic decreases in the activity of this enzyme in the tissues, but certain sites such as liver are affected more than others. Decreases in glutathione peroxidase correlate with lesions caused by selenium deficiency and appear useful in diagnosing selenium deficiency. Glutathione peroxidase is an important enzyme in destroying H2O2 and organic hydroperoxides such as lipid hydroperoxides. It therefore guards against oxidative damage to the cell membranes and other oxidant-sensitive sites in the cell. While this selenium-dependent system destroys lipid hydroperoxides and other peroxides, vitamin E is believed to protect against oxidant damage to membranes by preventing the formation of lipid hydroperoxides. A scheme is proposed, based on oxidant damage and its prevention, which accounts for the interaction between selenium, vitamin E, unsaturated lipids, sulfur-containing amino acids, and cell damaging agents such as oxidant stressors and toxicants such as silver and tri-o-cresyl phosphate. The background for such a scheme is reviewed."}
{"id": "PMID:1100438", "title": "Mechanisms of action of selenium and vitamin E in protection of biological membranes.", "content": "Opinions vary as to the mode of action of vitamin E and selenium. Some argue that they act as nonspecific biological antioxidants. Others propose that the functions of the two substances are distinct and that vitamin E acts as a true vitamin in addition to functioning as a lipid-soluble antioxidant. Support for the \"Biological Antioxidant Theory\" is largely circumstantial. However, lipoperoxides have been detected in adipose tissues of vitamin E-deficient animals, and increased rates of in vitro peroxidation have been demonstrated in homogenates of several tissues of selenium and vitamin E-deficient animals. The basis of the antioxygenic role of selenium in these systems was elucidated by the discovery of Rotruck et al. (1973) that selenium is a component of rat erythrocyte glutathione peroxidase. Further studies in this laboratory have demonstrated the important role of glutathione peroxidase in protection against the vitamin E- and selenium-deficiency disease of chicks, exudative diathesis, which results from increased capillary permeability. Also shown were the activities of both dietary selenium and vitamin E in prevention of ascorbate-induced peroxidation in mitochondrial and microsomal preparations from chick liver. Recent results demonstrate that both selenium and vitamin E are required to protect hepatic mitochondria and microsomes from peroxidative degradation. Dietary requirements of the chick for both nutrients for this function have been determined: approximately 0.06 ppm selenium in the presence of adequate vitamin E; 30-50 IU vitamin E per kg in the presence of adequate selenium.", "contents": "Mechanisms of action of selenium and vitamin E in protection of biological membranes. Opinions vary as to the mode of action of vitamin E and selenium. Some argue that they act as nonspecific biological antioxidants. Others propose that the functions of the two substances are distinct and that vitamin E acts as a true vitamin in addition to functioning as a lipid-soluble antioxidant. Support for the \"Biological Antioxidant Theory\" is largely circumstantial. However, lipoperoxides have been detected in adipose tissues of vitamin E-deficient animals, and increased rates of in vitro peroxidation have been demonstrated in homogenates of several tissues of selenium and vitamin E-deficient animals. The basis of the antioxygenic role of selenium in these systems was elucidated by the discovery of Rotruck et al. (1973) that selenium is a component of rat erythrocyte glutathione peroxidase. Further studies in this laboratory have demonstrated the important role of glutathione peroxidase in protection against the vitamin E- and selenium-deficiency disease of chicks, exudative diathesis, which results from increased capillary permeability. Also shown were the activities of both dietary selenium and vitamin E in prevention of ascorbate-induced peroxidation in mitochondrial and microsomal preparations from chick liver. Recent results demonstrate that both selenium and vitamin E are required to protect hepatic mitochondria and microsomes from peroxidative degradation. Dietary requirements of the chick for both nutrients for this function have been determined: approximately 0.06 ppm selenium in the presence of adequate vitamin E; 30-50 IU vitamin E per kg in the presence of adequate selenium."}
{"id": "PMID:1100441", "title": "In vitro production of the factors effective in the transfer of experimental autoimmune aspermatogenesis.", "content": "We studied the biological effects of the factors released into the culture medium by the lymphocytes from animals sensitized with testicular antigen. The supernatants from cultures of these lymphocytes were active in vitro in the migration inhibition tests because they transferred the sensitivity to normal spleen cells. In vivo, they transferred aspermatogenesis in the allogeneic and xenogeneic system. The results suggest a specific effect on the behaviour of macrophages rather than a direct cytotoxic effect of the active factors.", "contents": "In vitro production of the factors effective in the transfer of experimental autoimmune aspermatogenesis. We studied the biological effects of the factors released into the culture medium by the lymphocytes from animals sensitized with testicular antigen. The supernatants from cultures of these lymphocytes were active in vitro in the migration inhibition tests because they transferred the sensitivity to normal spleen cells. In vivo, they transferred aspermatogenesis in the allogeneic and xenogeneic system. The results suggest a specific effect on the behaviour of macrophages rather than a direct cytotoxic effect of the active factors."}
{"id": "PMID:1100442", "title": "Inhibition of haemopoietic stem cells by syngeneic lymphocytes treated with antilymphocyte serum.", "content": "Some aspects of the mechanisms by which antilymphocyte serum, its globulin fraction, or phytohaemagglutinin act to reduce the stem cell content of murine haemopoietic tissues were studied, using the method of clonal proliferation of stem cells in the spleens of lethally irradiated (830R) mice. The transplants were treated in vitro with ALS, ALG or PHA for 30 min. at 37 degrees C. Direct treatment of spleen or bone marrow cells with ALS or PHA resulted in pronounced inhibition of the colony-forming capacity of haemopoietic tissues in syngeneic irradiated recipients. The degree of inhibition was dependent on the concentration of ALS or PHA. It was found that the inhibition of CFC with ALS was mediated by lymphoid cells: bone marrow CFC were reduced after 30-min incubation at 37 degrees C with syngeneic lymph node or thymus cell pretreated with ALG and washed free of its excess. It was further demonstrated that ALG-treated thymocytes released, upon 30-min. incubation at 37 degrees C, some material capable of inhibiting bone marrow CFC. No release was observed with PHA-treated thymocytes. Some properties of the active supernatants are described and it is suggested to designate the observed inhibitory activity as Stem-cell Inhibitory Factor.", "contents": "Inhibition of haemopoietic stem cells by syngeneic lymphocytes treated with antilymphocyte serum. Some aspects of the mechanisms by which antilymphocyte serum, its globulin fraction, or phytohaemagglutinin act to reduce the stem cell content of murine haemopoietic tissues were studied, using the method of clonal proliferation of stem cells in the spleens of lethally irradiated (830R) mice. The transplants were treated in vitro with ALS, ALG or PHA for 30 min. at 37 degrees C. Direct treatment of spleen or bone marrow cells with ALS or PHA resulted in pronounced inhibition of the colony-forming capacity of haemopoietic tissues in syngeneic irradiated recipients. The degree of inhibition was dependent on the concentration of ALS or PHA. It was found that the inhibition of CFC with ALS was mediated by lymphoid cells: bone marrow CFC were reduced after 30-min incubation at 37 degrees C with syngeneic lymph node or thymus cell pretreated with ALG and washed free of its excess. It was further demonstrated that ALG-treated thymocytes released, upon 30-min. incubation at 37 degrees C, some material capable of inhibiting bone marrow CFC. No release was observed with PHA-treated thymocytes. Some properties of the active supernatants are described and it is suggested to designate the observed inhibitory activity as Stem-cell Inhibitory Factor."}
{"id": "PMID:1100443", "title": "Analysis of the Kinetics of the immune response by means of ionizing radiation.", "content": "The inhibitory kinetics of the primary immune response was studied by means of lethal irradiation. CBA mice were immunized with sheep red blood cells and 48, 72 and 96 hours later, they were irradiated with 750R. Afterwards, the number of direct plaque-forming cells was determined in their spleens during 58 to 66 hours at 4-hour intervals. After irradiation at 48 and 72 hours of the immune response, a plateau of different duration was obtained: 48 hours in the former and 24 hours in the latter case. In both cases, reduction in the PFC number began at about 96 hours of the resonse. The PFC reduction curves in these two series of experiments and those obtained 96 hours after irradiation and in control, non-irradiated mice were parallel. The mean half-time of the PFC number reduction as determined by these curves was 6 hours. The time of functioning of PFC in the spleen is discussed with respect to recruitment of proliferating precursors.", "contents": "Analysis of the Kinetics of the immune response by means of ionizing radiation. The inhibitory kinetics of the primary immune response was studied by means of lethal irradiation. CBA mice were immunized with sheep red blood cells and 48, 72 and 96 hours later, they were irradiated with 750R. Afterwards, the number of direct plaque-forming cells was determined in their spleens during 58 to 66 hours at 4-hour intervals. After irradiation at 48 and 72 hours of the immune response, a plateau of different duration was obtained: 48 hours in the former and 24 hours in the latter case. In both cases, reduction in the PFC number began at about 96 hours of the resonse. The PFC reduction curves in these two series of experiments and those obtained 96 hours after irradiation and in control, non-irradiated mice were parallel. The mean half-time of the PFC number reduction as determined by these curves was 6 hours. The time of functioning of PFC in the spleen is discussed with respect to recruitment of proliferating precursors."}
{"id": "PMID:1100444", "title": "Effects of various immunosuppressive drugs on the development of experimental renal infection in rats.", "content": "The effect of azathioprine, hydrocortisone and antihymocyte serum on the development of experimental colibacillary renal infection in rats was assessed by macroscopical findings in the kidneys, especially by determining the number of microbes in the infected organ, on day 7 after injection of the infectious agent. The greatest multiplication of bacteria and so the most pronounced macroscopical changes occurred after ALS treatment. There was statistically significant difference between ALS-treated group and the control group, whereas the differences between controls and the other experimental groups (Imuran, hydrocortisone) were non-significant.", "contents": "Effects of various immunosuppressive drugs on the development of experimental renal infection in rats. The effect of azathioprine, hydrocortisone and antihymocyte serum on the development of experimental colibacillary renal infection in rats was assessed by macroscopical findings in the kidneys, especially by determining the number of microbes in the infected organ, on day 7 after injection of the infectious agent. The greatest multiplication of bacteria and so the most pronounced macroscopical changes occurred after ALS treatment. There was statistically significant difference between ALS-treated group and the control group, whereas the differences between controls and the other experimental groups (Imuran, hydrocortisone) were non-significant."}
{"id": "PMID:1100459", "title": "Effect of intracisternal insulin on plasma glucose and insulin in the dog.", "content": "The intracisternal administration of insulin (0.2 U./kg.) to anesthetized dogs resulted in an increase of arterial immunoreactive insulin and a decrease of plasma glucose relative to a control injection. The arterial responses were significantly attenuated when the insulin was administered to the cisternum of subdiaphragmatically vagotomized dogs. When cerebrospinal fluid glucose was lowered by injecting pneumococcal neuraminidase intracisternally, no peripheral hyperinsulinemia resulted, indicating that increased spinal fluid insulin and its consequent increase of glucose uptake, rather than decreased spinal fluid glucose, is necessary to elicit the vagally mediated insulin secretion and hypoglycemia. It is hypothesized that increased spinal fluid insulin causes an increased glucose uptake of some glucoregulatory area of the brain and that the elicited reflex is vagally mediated pancreatic insulin secretion.", "contents": "Effect of intracisternal insulin on plasma glucose and insulin in the dog. The intracisternal administration of insulin (0.2 U./kg.) to anesthetized dogs resulted in an increase of arterial immunoreactive insulin and a decrease of plasma glucose relative to a control injection. The arterial responses were significantly attenuated when the insulin was administered to the cisternum of subdiaphragmatically vagotomized dogs. When cerebrospinal fluid glucose was lowered by injecting pneumococcal neuraminidase intracisternally, no peripheral hyperinsulinemia resulted, indicating that increased spinal fluid insulin and its consequent increase of glucose uptake, rather than decreased spinal fluid glucose, is necessary to elicit the vagally mediated insulin secretion and hypoglycemia. It is hypothesized that increased spinal fluid insulin causes an increased glucose uptake of some glucoregulatory area of the brain and that the elicited reflex is vagally mediated pancreatic insulin secretion."}
{"id": "PMID:1100458", "title": "Dynamics of insulin release and microtubular-microfilamentous system. VII. Do microfilaments provide the motive force for the translocation and extrusion of beta granules?", "content": "The active role played by beta-cell microfilamentous structures in the dynamics of insulin secretion was investigated by examining the influence of cytochalasin B upon various parameters of hormonal release by the isolated perfused rat pancreas. The view that the cytochalasin-induced changes in insulin release are due to a primary biophysical effect on microfilaments, rather than to an unrelated biochemical alteration of the beta-cell glucose-sensor device, was strengthened by the following observations: (1) the onset and disappearance of the cytochalasin B-induced facilitating action upon insulin release followed a time-course parallel to that characterizing the ultrastructural changes provoked by the drug in the distribution of beta-cell microfilamentous material; and (2) cytochalasin B facilitated leucine-induced insulin release in the presence of a very low glucose concentration. The mold metabolite was also found to transform transient secretory responses into biphasic ones and to prevent the reduction that normally affects the early response to insulinotropic agents when the pancreas is stimulated a few minutes after a prior and short exposure to glucose. The release of insulin evoked by either glucose or gliclazide was abolished in the absence of extracellular calcium, whether in the presence or absence of cytochalasin B. Theophylline and cytochalasin B exerted a synergistic effect upon glucose-induced insulin release. These data support the concept that calcium-dependent contractile events involving cytochalasin B-sensitive microfilamentous structures provide the motive force for both the intracellular translocation and exocytotic release of beta granules.", "contents": "Dynamics of insulin release and microtubular-microfilamentous system. VII. Do microfilaments provide the motive force for the translocation and extrusion of beta granules? The active role played by beta-cell microfilamentous structures in the dynamics of insulin secretion was investigated by examining the influence of cytochalasin B upon various parameters of hormonal release by the isolated perfused rat pancreas. The view that the cytochalasin-induced changes in insulin release are due to a primary biophysical effect on microfilaments, rather than to an unrelated biochemical alteration of the beta-cell glucose-sensor device, was strengthened by the following observations: (1) the onset and disappearance of the cytochalasin B-induced facilitating action upon insulin release followed a time-course parallel to that characterizing the ultrastructural changes provoked by the drug in the distribution of beta-cell microfilamentous material; and (2) cytochalasin B facilitated leucine-induced insulin release in the presence of a very low glucose concentration. The mold metabolite was also found to transform transient secretory responses into biphasic ones and to prevent the reduction that normally affects the early response to insulinotropic agents when the pancreas is stimulated a few minutes after a prior and short exposure to glucose. The release of insulin evoked by either glucose or gliclazide was abolished in the absence of extracellular calcium, whether in the presence or absence of cytochalasin B. Theophylline and cytochalasin B exerted a synergistic effect upon glucose-induced insulin release. These data support the concept that calcium-dependent contractile events involving cytochalasin B-sensitive microfilamentous structures provide the motive force for both the intracellular translocation and exocytotic release of beta granules."}
{"id": "PMID:1100460", "title": "Effect of cerebral intraventricular insulin on pancreatic insulin secretion in the dog.", "content": "The effect of cerebral intraventricular insulin on pancreatic insulin secretion was investigated. An extracorporeal pancreatic blood circuit was established after laparotomy to monitor blood flow and insulin concentration directly from the superior pancreaticoduodenal vein. Phentolamine was infused throughout (0.2 mg./min. intravenously) to block alpha-adrenergic effects of any catecholamine secretion induced by surgical stress. Glucose (1.5 mg./kg./min. intravenously) was infused to maintain a constant baseline stimulation of insulin secretion. Six dogs received insulin and six control dogs received saline through a spinal needle stereotaxically placed into the left lateral cerebral ventricle. After central injection of insulin (0.2 U./kg.) there was a significant increase of pancreatic output as early as five minutes. It is concluded that the pancreatic beta-cells are under the influence of insulin-sensitive cells of the CNS.", "contents": "Effect of cerebral intraventricular insulin on pancreatic insulin secretion in the dog. The effect of cerebral intraventricular insulin on pancreatic insulin secretion was investigated. An extracorporeal pancreatic blood circuit was established after laparotomy to monitor blood flow and insulin concentration directly from the superior pancreaticoduodenal vein. Phentolamine was infused throughout (0.2 mg./min. intravenously) to block alpha-adrenergic effects of any catecholamine secretion induced by surgical stress. Glucose (1.5 mg./kg./min. intravenously) was infused to maintain a constant baseline stimulation of insulin secretion. Six dogs received insulin and six control dogs received saline through a spinal needle stereotaxically placed into the left lateral cerebral ventricle. After central injection of insulin (0.2 U./kg.) there was a significant increase of pancreatic output as early as five minutes. It is concluded that the pancreatic beta-cells are under the influence of insulin-sensitive cells of the CNS."}
{"id": "PMID:1100461", "title": "Ethanol-induced alterations of glucose tolerance, postglucose hypoglycemia, and insulin secretion in normal, obese, and diabetic subjects.", "content": "Ethanol at an average blood concentration of 1 mg. per milliliter enhanced the immediate (first-phase) and prolonged (second-phase) insulin response to an intravenous glucose load in nonfasting normal human subjects. Simultaneously, the glucose disposal rate was increased and the postglucose hypoglycemia was accentuated, resulting in definite hypoglycemic symptoms in some individuals. Oral glucose tolerance was not changed by ethanol administration, but the thirty-minute blood glucose and plasma insulin values were increased, suggesting that alcohol might accelerate the absorption of glucose from the gut. Ethanol given orally during evening hours (1.5 gm. per kilogram) caused a nocturnal hyperinsulinemia and a decrease of blood glucose, but not an actual hypoglycemia. Oral glucose tolerance and plasma insulin response tested the next morning, when ethanol had disappeared from the blood, were not influenced by drinking the previous evening. The K-value of intravenous glucose was increased at this time, however. When alcohol was administered for one week at a dose corresponding to 25 per cent of daily calories and substituting for fat, both the oral and intravenous glucose tolerances were impaired in each subject but the insulin response remained unchaged. In obese nondiabetic subjects, ethanol did not potentiate the early insulin response to intravenous glucose but it increased the second phase of insulin secretion in response to sustained hyperglycemia. In contrast to conditions in nonobese subjects, the glucose disposal rate was not incresed and postglucose hypoglycemia was not accentuated by ethanol in overweight subjects. In insulin-deficient diabetic patients the absent early insulin response could not be restored by ethanol, and the late component of insulin release was little increased by alcohol infusion. Ethanol did not improve the glucose utilization of diabetic patients.", "contents": "Ethanol-induced alterations of glucose tolerance, postglucose hypoglycemia, and insulin secretion in normal, obese, and diabetic subjects. Ethanol at an average blood concentration of 1 mg. per milliliter enhanced the immediate (first-phase) and prolonged (second-phase) insulin response to an intravenous glucose load in nonfasting normal human subjects. Simultaneously, the glucose disposal rate was increased and the postglucose hypoglycemia was accentuated, resulting in definite hypoglycemic symptoms in some individuals. Oral glucose tolerance was not changed by ethanol administration, but the thirty-minute blood glucose and plasma insulin values were increased, suggesting that alcohol might accelerate the absorption of glucose from the gut. Ethanol given orally during evening hours (1.5 gm. per kilogram) caused a nocturnal hyperinsulinemia and a decrease of blood glucose, but not an actual hypoglycemia. Oral glucose tolerance and plasma insulin response tested the next morning, when ethanol had disappeared from the blood, were not influenced by drinking the previous evening. The K-value of intravenous glucose was increased at this time, however. When alcohol was administered for one week at a dose corresponding to 25 per cent of daily calories and substituting for fat, both the oral and intravenous glucose tolerances were impaired in each subject but the insulin response remained unchaged. In obese nondiabetic subjects, ethanol did not potentiate the early insulin response to intravenous glucose but it increased the second phase of insulin secretion in response to sustained hyperglycemia. In contrast to conditions in nonobese subjects, the glucose disposal rate was not incresed and postglucose hypoglycemia was not accentuated by ethanol in overweight subjects. In insulin-deficient diabetic patients the absent early insulin response could not be restored by ethanol, and the late component of insulin release was little increased by alcohol infusion. Ethanol did not improve the glucose utilization of diabetic patients."}
{"id": "PMID:1100462", "title": "The G cell population of the pyloric antrum of the cat.", "content": "The method used in this study to quantify the G cell population of the cat is similar to the technique used for measuring the parietal cell mass in the stomach of rats and man. The method may be used on the human antrum. Mucosal sampling technique and immunofluorescence methods are described. The method is reproducible. G cell distribution in the antrum was not uniform and the highest concentrations were found along the lesser curvature. The G cell population was 10.27, 12.66, 12.87, 12.88 and 15.42 million cells (mean 12.8 x  10(6)) per antrum in each of the five cats studied.", "contents": "The G cell population of the pyloric antrum of the cat. The method used in this study to quantify the G cell population of the cat is similar to the technique used for measuring the parietal cell mass in the stomach of rats and man. The method may be used on the human antrum. Mucosal sampling technique and immunofluorescence methods are described. The method is reproducible. G cell distribution in the antrum was not uniform and the highest concentrations were found along the lesser curvature. The G cell population was 10.27, 12.66, 12.87, 12.88 and 15.42 million cells (mean 12.8 x  10(6)) per antrum in each of the five cats studied."}
{"id": "PMID:1100469", "title": "Hepatocellular uptake of bile acids in the dog: Evidence for a common carrier-mediated transport system. An indicator dilution study.", "content": "The purpose of this study was to compare the uptake of taurocholate (TC) by the liver of the dog to that of taurochenodeoxycholate (TCDC) and to examine the influence of TCDC on TC uptake. The uptake of these bile acids by the liver of the intact was studied with the multiple indicator dilution method, using 51Cr-labeled red blood cells as a vascular indicator, 125I-labeled albumin as an extravascular reference, and one or two labeled bile acids. The analysis of the dilution curves fitted well a three-compartment model, and no return of the extracted bile acids to the extracellular space could be detected. The initial space of distributin of TC in the presence of a fixed dose of TCDC was 1.25 +/- 0.09 (SD) times greater than that of albumin. Analysis of the data for TC uptake in the presence of TCDC was consistent with Michaelis-Menten kinetics, as previously established for TC alone. The calculated initial maximal velocity of uptake (Vmax) of TC in the precence of TCDC was 4.92 +/- 1.36 (SD) mumoles per sec per 100 g of liver. This value is not significantly different from the Vmax previously calculated for TC alone. The apparent KD (dose of TC yielding half-maximal velocity) was 18.10 +/- 6.14 (SD) mumoles per 100 g of liver, a value significantly higher than that obtained in the absence of TCDC. A K1 (the Michaelis-type constant characterizing the inhibition of TC uptake by TCDC) of 2.71 +/- 1.08 (SD) mumoles of TCDC per 100 g of liver was calculated. These observations are consistent with competitive inhibition of TC uptake by TCDC, and thus with the existence of a common carrier-mediated transport system for TC and TCDC. Estimation of the kinetic characteristics of the two bile acids from dilution curves obtained simultaneously showed that the affinity for the carrier was higher for TCDC than for TC, but that the maximal capacity was lower for TCDC than for TC.", "contents": "Hepatocellular uptake of bile acids in the dog: Evidence for a common carrier-mediated transport system. An indicator dilution study. The purpose of this study was to compare the uptake of taurocholate (TC) by the liver of the dog to that of taurochenodeoxycholate (TCDC) and to examine the influence of TCDC on TC uptake. The uptake of these bile acids by the liver of the intact was studied with the multiple indicator dilution method, using 51Cr-labeled red blood cells as a vascular indicator, 125I-labeled albumin as an extravascular reference, and one or two labeled bile acids. The analysis of the dilution curves fitted well a three-compartment model, and no return of the extracted bile acids to the extracellular space could be detected. The initial space of distributin of TC in the presence of a fixed dose of TCDC was 1.25 +/- 0.09 (SD) times greater than that of albumin. Analysis of the data for TC uptake in the presence of TCDC was consistent with Michaelis-Menten kinetics, as previously established for TC alone. The calculated initial maximal velocity of uptake (Vmax) of TC in the precence of TCDC was 4.92 +/- 1.36 (SD) mumoles per sec per 100 g of liver. This value is not significantly different from the Vmax previously calculated for TC alone. The apparent KD (dose of TC yielding half-maximal velocity) was 18.10 +/- 6.14 (SD) mumoles per 100 g of liver, a value significantly higher than that obtained in the absence of TCDC. A K1 (the Michaelis-type constant characterizing the inhibition of TC uptake by TCDC) of 2.71 +/- 1.08 (SD) mumoles of TCDC per 100 g of liver was calculated. These observations are consistent with competitive inhibition of TC uptake by TCDC, and thus with the existence of a common carrier-mediated transport system for TC and TCDC. Estimation of the kinetic characteristics of the two bile acids from dilution curves obtained simultaneously showed that the affinity for the carrier was higher for TCDC than for TC, but that the maximal capacity was lower for TCDC than for TC."}
{"id": "PMID:1100468", "title": "Serum immunoglobulin M concentrations following bowel resection in chronic inflammatory bowel disease.", "content": "Serum immunoglobulin alterations were characterized in 20 patients with chronic inflammatory bowel disease (CIBD) undergoing bowel resection and in 11 control subjects undergoing intra-abdominal operations. Serum immunoglobulin M (IgM) values became elevated during the 2nd week in both groups with individual serum IgM concentrations reaching as high as 9 times preoperative values. The IgM increment, which was 19 S polyclonal immunoglobulin, was significantly greater in the CIBD group than in controls. Because of a recent suggestion that this IgM rise might be related to the pathogenesis of CIBD, the affinity of this IgM for colon was studied in two ways. Serum anticolon antibody titers did not change after surgery nor did IgM, isolated from serum after surgery and labeled with 125I, bind to homogenates of normal or CIBD colon. Titers of heterophile antibody and antibodies directed against Escherichia coli, Bacteroides, and blood group antigens increased in many patients. In most instances, these increments were shown to be antibody of the IgM class by reduction with 2-mercaptoethanol. These data indicate that the IgM changes after abdominal surgery (1) are not specific for CIBD patients, (2) do not represent antibody directed against colon antigens, and (3) include increased titers of several IgM antibodies. These findings might be explained by a response to bacterial or tissue antigens released at or after the time of surgery.", "contents": "Serum immunoglobulin M concentrations following bowel resection in chronic inflammatory bowel disease. Serum immunoglobulin alterations were characterized in 20 patients with chronic inflammatory bowel disease (CIBD) undergoing bowel resection and in 11 control subjects undergoing intra-abdominal operations. Serum immunoglobulin M (IgM) values became elevated during the 2nd week in both groups with individual serum IgM concentrations reaching as high as 9 times preoperative values. The IgM increment, which was 19 S polyclonal immunoglobulin, was significantly greater in the CIBD group than in controls. Because of a recent suggestion that this IgM rise might be related to the pathogenesis of CIBD, the affinity of this IgM for colon was studied in two ways. Serum anticolon antibody titers did not change after surgery nor did IgM, isolated from serum after surgery and labeled with 125I, bind to homogenates of normal or CIBD colon. Titers of heterophile antibody and antibodies directed against Escherichia coli, Bacteroides, and blood group antigens increased in many patients. In most instances, these increments were shown to be antibody of the IgM class by reduction with 2-mercaptoethanol. These data indicate that the IgM changes after abdominal surgery (1) are not specific for CIBD patients, (2) do not represent antibody directed against colon antigens, and (3) include increased titers of several IgM antibodies. These findings might be explained by a response to bacterial or tissue antigens released at or after the time of surgery."}
{"id": "PMID:1100472", "title": "[On the beginnings of hormonal contraception (author's transl)].", "content": "The possibility of hormonal contraception was postulated as early as 1919 by the physiologist Ludwig Haberlandt in Innsbruck. The same year, he began to test his hypothesis in animal experiments. In 1924 he succeeded in his efforts to render mice infertil by orally administering ovarian and placental extracts. He failed to have his method tried in women.", "contents": "[On the beginnings of hormonal contraception (author's transl)]. The possibility of hormonal contraception was postulated as early as 1919 by the physiologist Ludwig Haberlandt in Innsbruck. The same year, he began to test his hypothesis in animal experiments. In 1924 he succeeded in his efforts to render mice infertil by orally administering ovarian and placental extracts. He failed to have his method tried in women."}
{"id": "PMID:1100487", "title": "The autonomic nervous system and its relationship to tubal ovum transport--a reappraisal.", "content": "The role of the autonomic nervous system in controlling ovum transport remains obscure. Although not studied extensively in the oviduct, the para-sympathetic nervous system does not appear to significantly influence ovum transport. The sympathetic nervous system of the oviduct and its pharmacology have been studied more thoroughly. Despite this, little information is available concerning cellular mechanisims of adrenergically altered motility or transport. In spite of much speculation, the weight of evidence suggests that, at least in the rabbit, the sympathetic nervous system plays a minor role in the control of ovum transport.", "contents": "The autonomic nervous system and its relationship to tubal ovum transport--a reappraisal. The role of the autonomic nervous system in controlling ovum transport remains obscure. Although not studied extensively in the oviduct, the para-sympathetic nervous system does not appear to significantly influence ovum transport. The sympathetic nervous system of the oviduct and its pharmacology have been studied more thoroughly. Despite this, little information is available concerning cellular mechanisims of adrenergically altered motility or transport. In spite of much speculation, the weight of evidence suggests that, at least in the rabbit, the sympathetic nervous system plays a minor role in the control of ovum transport."}
{"id": "PMID:1100488", "title": "Sperm transport to and survival in the human fallopian tube.", "content": "A review is given on sperm migration to and sperm survival within the human Fallopian tube. Sperm migration from the external os can be very fast. The survival time of spermatozoa in the oviduct has been demonstrated to be 85 h. Spermatozoa normally enter the abdominal cavity through the open fimbriated end. Laterally closed oviducts retain spermatozoa resulting in a larger number of spermatozoa than in the normal oviduct, where the number of sperm at the site of fertilization is very low. The morphology of spermatozoa reaching the ampulla of the oviduct is mostly normal, which seems to be based on the correlation between normal morphology and good motility. Spermatozoa within the abdominal cavity do not cause antibody formation of any importance for the fertility of the woman.", "contents": "Sperm transport to and survival in the human fallopian tube. A review is given on sperm migration to and sperm survival within the human Fallopian tube. Sperm migration from the external os can be very fast. The survival time of spermatozoa in the oviduct has been demonstrated to be 85 h. Spermatozoa normally enter the abdominal cavity through the open fimbriated end. Laterally closed oviducts retain spermatozoa resulting in a larger number of spermatozoa than in the normal oviduct, where the number of sperm at the site of fertilization is very low. The morphology of spermatozoa reaching the ampulla of the oviduct is mostly normal, which seems to be based on the correlation between normal morphology and good motility. Spermatozoa within the abdominal cavity do not cause antibody formation of any importance for the fertility of the woman."}
{"id": "PMID:1100491", "title": "The isolation of protoplasts of the fission yeast Schizosaccharomyces by Trichoderma viride and snail enzymes.", "content": "The formation of protoplasts of the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces versatilis after the combined application of snail enzymes and Trichoderma viride enzymes in an osmotic stabilizer (0.4M KCl, pH 5.5) was studied by light and electron microscopy. The effect of the enzymes used leads during 30 min to the formation of 100% protoplast population. Using electron microscopy no original walls or wall remnants were detected in the suspension of protoplasts. Protoplasts are viable and in liquid nutrient medium they regenerate cell walls and revert into normal cells. Such a protoplast population may be useful for biochemical study of protoplast metabolism by quantitative methods as well as for the chemical study of regenerating cell walls.", "contents": "The isolation of protoplasts of the fission yeast Schizosaccharomyces by Trichoderma viride and snail enzymes. The formation of protoplasts of the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces versatilis after the combined application of snail enzymes and Trichoderma viride enzymes in an osmotic stabilizer (0.4M KCl, pH 5.5) was studied by light and electron microscopy. The effect of the enzymes used leads during 30 min to the formation of 100% protoplast population. Using electron microscopy no original walls or wall remnants were detected in the suspension of protoplasts. Protoplasts are viable and in liquid nutrient medium they regenerate cell walls and revert into normal cells. Such a protoplast population may be useful for biochemical study of protoplast metabolism by quantitative methods as well as for the chemical study of regenerating cell walls."}
{"id": "PMID:1100492", "title": "Penicillinamidohydrolase in Escherichia coli. II. Synthesis of the enzyme, kinetics and specificity of its induction and the effect of O2.", "content": "The differential rate of synthesis of penicillinamidohydrolase (penicillin acylase -- EC 3.5.1.11) was studied in Escherichia coli growing in some chemically defined media and in a complex medium. The enzyme is synthesized at a constant rate only during the exponential phase of growth of cells. Its synthesis is induced most effectively (with respect to quantity) by phenylacetic acid. The induction lag of the enzyme synthesis in a medium with acetate corresponds to two generation times. The highest rate of the enzyme synthesis is reached in a medium containing phenylacetic acid as the only source of carbon and energy. The enzyme synthesis is fully repressed by an increased concentration of dissolved oxygen in the medium, even when Escherichia coli is cultivated in the medium with phenylacetic acid as the only carbon and energy source.", "contents": "Penicillinamidohydrolase in Escherichia coli. II. Synthesis of the enzyme, kinetics and specificity of its induction and the effect of O2. The differential rate of synthesis of penicillinamidohydrolase (penicillin acylase -- EC 3.5.1.11) was studied in Escherichia coli growing in some chemically defined media and in a complex medium. The enzyme is synthesized at a constant rate only during the exponential phase of growth of cells. Its synthesis is induced most effectively (with respect to quantity) by phenylacetic acid. The induction lag of the enzyme synthesis in a medium with acetate corresponds to two generation times. The highest rate of the enzyme synthesis is reached in a medium containing phenylacetic acid as the only source of carbon and energy. The enzyme synthesis is fully repressed by an increased concentration of dissolved oxygen in the medium, even when Escherichia coli is cultivated in the medium with phenylacetic acid as the only carbon and energy source."}
{"id": "PMID:1100493", "title": "The effect of medium composition on the production of valine by Corynebacterium 9366 - EMS/184.", "content": "The auxotrophic mutant Corynebacterium 9366-EMS/184, requiring isoleucine accumulates about 22 g of L-valine per litre after a 96 h cultivation in a medium containing 15% sucrose and 1% ammonium acetate.", "contents": "The effect of medium composition on the production of valine by Corynebacterium 9366 - EMS/184. The auxotrophic mutant Corynebacterium 9366-EMS/184, requiring isoleucine accumulates about 22 g of L-valine per litre after a 96 h cultivation in a medium containing 15% sucrose and 1% ammonium acetate."}
{"id": "PMID:1100503", "title": "Formation of a complex between yeast proteinases A and B.", "content": "Sephadex G-100 gel filtration of a purified mixture of tryptophan-synthase-inactivating enzymes I and II from Saccharomyces cerevisiae, which had previously been identified as yeast proteinases A and B, yields two coincident peaks of tryptophan-synthase-inactivating activity and proteinase A and B activities. The peaks correspond to molecular weights of approximately 100 000 and 50 000. It could be demonstrated, with purified proteinases A and B, that the high molecular weight peaks is due to the formation of a stoichiometric complex between proteinases A and B. The AB complex can easily be separated into its constituent enzymes by DEAE-Sephadex chromatography. When in the AB complex, proteinase B molecules still bind the specific proteinase B inhibitor from yeast.", "contents": "Formation of a complex between yeast proteinases A and B. Sephadex G-100 gel filtration of a purified mixture of tryptophan-synthase-inactivating enzymes I and II from Saccharomyces cerevisiae, which had previously been identified as yeast proteinases A and B, yields two coincident peaks of tryptophan-synthase-inactivating activity and proteinase A and B activities. The peaks correspond to molecular weights of approximately 100 000 and 50 000. It could be demonstrated, with purified proteinases A and B, that the high molecular weight peaks is due to the formation of a stoichiometric complex between proteinases A and B. The AB complex can easily be separated into its constituent enzymes by DEAE-Sephadex chromatography. When in the AB complex, proteinase B molecules still bind the specific proteinase B inhibitor from yeast."}
{"id": "PMID:1100504", "title": "Synthesis of a chemically reactive analog of the nonsense codon U-G-A. Its reaction with ribosomes of Escherichia coli.", "content": "Nitrophenylated 5'-uridylic acid could be employed as primer in a polyribonucleotide nucleotidyltransferase (Micrococcus luteus) reaction to yield 5'-nitrophenylated Pu-G-A. After reduction of the nitrophenyl moiety and subsequent bromoacetylation, a 5'-bromoacetamido-phenyl-phosphorylated U-G-A was obtained, which could be used as an affinity label for the ribosomal binding site of the nonsense codon. If freshly prepared active ribosomes were employed in the incubation mixtures, the U-G-A analog reacted exclusively with one protein, which is tentatively identified as protein S18. Exposure of ribosomes to low temperatures gave rise to a reaction of the U-G-A label with another protein, which was identified as protein S4, the ram gene product. The results of the affinity labeling experiments with the chemically reactive U-G-A derivative are very similar to that obtained with a corresponding derivative of the initiation codon A-U-G (O. Pongs and E. Lanka (1975) Proc. Natl. Acad. Sci. U.S.A. 75, 1505-1509), which suggests that 70S ribosomes have one preferential codon binding site.", "contents": "Synthesis of a chemically reactive analog of the nonsense codon U-G-A. Its reaction with ribosomes of Escherichia coli. Nitrophenylated 5'-uridylic acid could be employed as primer in a polyribonucleotide nucleotidyltransferase (Micrococcus luteus) reaction to yield 5'-nitrophenylated Pu-G-A. After reduction of the nitrophenyl moiety and subsequent bromoacetylation, a 5'-bromoacetamido-phenyl-phosphorylated U-G-A was obtained, which could be used as an affinity label for the ribosomal binding site of the nonsense codon. If freshly prepared active ribosomes were employed in the incubation mixtures, the U-G-A analog reacted exclusively with one protein, which is tentatively identified as protein S18. Exposure of ribosomes to low temperatures gave rise to a reaction of the U-G-A label with another protein, which was identified as protein S4, the ram gene product. The results of the affinity labeling experiments with the chemically reactive U-G-A derivative are very similar to that obtained with a corresponding derivative of the initiation codon A-U-G (O. Pongs and E. Lanka (1975) Proc. Natl. Acad. Sci. U.S.A. 75, 1505-1509), which suggests that 70S ribosomes have one preferential codon binding site."}
{"id": "PMID:1100506", "title": "The primary structure of the 5s rRNA binding protein L25 of Escherichia coli ribosomes.", "content": "The primary structure of protein L25 from the large subunit of Escherichia coli ribosomes was determined by isolation and analysis of peptides obtained after cleavage of the protein with trypsin, thermolysin and Staphylococcus protease as well as by Edman degradation of the intact protein and of a CNBr peptide. The complete amino acid sequence is shown in Fig. 4. There are sequence homologies within protein L25 (Table 6) as well as between protein L25 and other ribosomal proteins (Table 5).", "contents": "The primary structure of the 5s rRNA binding protein L25 of Escherichia coli ribosomes. The primary structure of protein L25 from the large subunit of Escherichia coli ribosomes was determined by isolation and analysis of peptides obtained after cleavage of the protein with trypsin, thermolysin and Staphylococcus protease as well as by Edman degradation of the intact protein and of a CNBr peptide. The complete amino acid sequence is shown in Fig. 4. There are sequence homologies within protein L25 (Table 6) as well as between protein L25 and other ribosomal proteins (Table 5)."}
{"id": "PMID:1100507", "title": "Properties of Isoaccepting tRNALys, tRNAGlu and tRNAGln from rabbit Reticulocytes and liver. Multiplicity, Codon Recognition and Inactivation by Iodine.", "content": "The three tRNA species from rabbit liver and reticulocytes, each corresponding to the codons XAA and XAG(X equals A, G, C) were investigated. The elution patterns of the isoaccepting tRNA subspecies after separation by reversedphase chromatography followed by determination of amino acid acceptance have common and differing characteristics. Three subspecies of tRNALys, two of tRNAGlu and three of tRNAGln have been found in reticulocytes, wheras two subspecies of tRNALys, three of tRNAGlu and four of tRNAGln have been determined in rabbit liver. Examination of codon recognition by means of a ribosome binding assay showed that each subspecies binds exclusively in the presence of only one of the two possible triplets; i.e. wobbling was not found. It has been assumed that this high specificity, which is not to be expected according to the \"wobble hypothesis\", is due to a 2-thiouracil base in the first position of the anticodon. This was supported by oxidation experiments with iodine. Treatment with iodine significantly reduces the aminoacylation capacity of all subspecies that show specific binding with triplets ending in adenosine. We describe and compare for the first time the characteristics of eucaryotic tRNA species which occupy homologous positions in the codon table. Thus it can be seen that all the investigated subspecies have rigorously specific codon recognition in common, wherby \"wobbling\" can probably be excluded by a principle that is valid for all the investigated species.", "contents": "Properties of Isoaccepting tRNALys, tRNAGlu and tRNAGln from rabbit Reticulocytes and liver. Multiplicity, Codon Recognition and Inactivation by Iodine. The three tRNA species from rabbit liver and reticulocytes, each corresponding to the codons XAA and XAG(X equals A, G, C) were investigated. The elution patterns of the isoaccepting tRNA subspecies after separation by reversedphase chromatography followed by determination of amino acid acceptance have common and differing characteristics. Three subspecies of tRNALys, two of tRNAGlu and three of tRNAGln have been found in reticulocytes, wheras two subspecies of tRNALys, three of tRNAGlu and four of tRNAGln have been determined in rabbit liver. Examination of codon recognition by means of a ribosome binding assay showed that each subspecies binds exclusively in the presence of only one of the two possible triplets; i.e. wobbling was not found. It has been assumed that this high specificity, which is not to be expected according to the \"wobble hypothesis\", is due to a 2-thiouracil base in the first position of the anticodon. This was supported by oxidation experiments with iodine. Treatment with iodine significantly reduces the aminoacylation capacity of all subspecies that show specific binding with triplets ending in adenosine. We describe and compare for the first time the characteristics of eucaryotic tRNA species which occupy homologous positions in the codon table. Thus it can be seen that all the investigated subspecies have rigorously specific codon recognition in common, wherby \"wobbling\" can probably be excluded by a principle that is valid for all the investigated species."}
{"id": "PMID:1100508", "title": "B-chain shortening of matrix-bound insulin with pepsin, II. Preparation and properties of camel des-pentapeptide (B26-30)- and des-PheB1-des-pentapeptide (B26-30)- insulin.", "content": "Digestion of matrix-bound camel insulin with pepsin was found to be restricted to the cleavage of the peptide bond between phenylalanine(B25) and tyrosine(B26). Purification of the camel des-pentapeptide(B26-30)-insulin obtained was achieved by gel filtration and ion exchange chromatography, yielding a molecularly uniform product. In the same way, camel des-PheB1-insulin prepared according to a method described for bovine insulin was hydrolyzed with pepsin and purified to give the des-PheB1-despentapeptide(B26-30)-insulin. The biological activity of these modified camel insulins was found to be as much reduced as the corresponding bovine insulin analogues. However, the reduced biological activity of camel des-PheB1-insulin (60%) was a contrast to the fully active bovine des-PheB1-insulin.", "contents": "B-chain shortening of matrix-bound insulin with pepsin, II. Preparation and properties of camel des-pentapeptide (B26-30)- and des-PheB1-des-pentapeptide (B26-30)- insulin. Digestion of matrix-bound camel insulin with pepsin was found to be restricted to the cleavage of the peptide bond between phenylalanine(B25) and tyrosine(B26). Purification of the camel des-pentapeptide(B26-30)-insulin obtained was achieved by gel filtration and ion exchange chromatography, yielding a molecularly uniform product. In the same way, camel des-PheB1-insulin prepared according to a method described for bovine insulin was hydrolyzed with pepsin and purified to give the des-PheB1-despentapeptide(B26-30)-insulin. The biological activity of these modified camel insulins was found to be as much reduced as the corresponding bovine insulin analogues. However, the reduced biological activity of camel des-PheB1-insulin (60%) was a contrast to the fully active bovine des-PheB1-insulin."}
{"id": "PMID:1100514", "title": "Pathology of cerebral embolization caused by nonthrombotic agents.", "content": "Although apparently infrequent, a variety of agents other than thrombotic material are known to cause cerebral embolization, often with serious consequences. The emboli may originate in different parts of the body or may be introduced from outside under diverse circumstances. The pathologic aspects of these individual embolic phenomena are described with particular emphasis on their recognition at autopsy. Adequate autopsy study appears to be the most important source of information for further elucidation of the incidence, mechanism, and sequelae of these heterogeneous embolic lesions of the brain. Such information is essential for clinical evaluation, management, and, more importantly, prevention of some of these potentially serious embolic phenomena.", "contents": "Pathology of cerebral embolization caused by nonthrombotic agents. Although apparently infrequent, a variety of agents other than thrombotic material are known to cause cerebral embolization, often with serious consequences. The emboli may originate in different parts of the body or may be introduced from outside under diverse circumstances. The pathologic aspects of these individual embolic phenomena are described with particular emphasis on their recognition at autopsy. Adequate autopsy study appears to be the most important source of information for further elucidation of the incidence, mechanism, and sequelae of these heterogeneous embolic lesions of the brain. Such information is essential for clinical evaluation, management, and, more importantly, prevention of some of these potentially serious embolic phenomena."}
{"id": "PMID:1100515", "title": "Vascular structures in brain tumors.", "content": "The blood vessels within brain tumors show alterations from the normal anatomy. Some of these seem to be related to an increased capacity to transfer materials between the lumen and the parenchyma and are probably intimately connected with the edema associated with the tumor. These alterations include fenestration, widened intercellular junctions, increase in pinocytotic vesicles, and infolding of the luminal surface. Other alterations are observed but their function is not as clear. The latter include an increase in the number of tubular bodies, the appearance of tubular structures within vacuoles, tubular arrays within the nuclear envelope and rough endoplasmic reticulum, and endothelial proliferation, among others.", "contents": "Vascular structures in brain tumors. The blood vessels within brain tumors show alterations from the normal anatomy. Some of these seem to be related to an increased capacity to transfer materials between the lumen and the parenchyma and are probably intimately connected with the edema associated with the tumor. These alterations include fenestration, widened intercellular junctions, increase in pinocytotic vesicles, and infolding of the luminal surface. Other alterations are observed but their function is not as clear. The latter include an increase in the number of tubular bodies, the appearance of tubular structures within vacuoles, tubular arrays within the nuclear envelope and rough endoplasmic reticulum, and endothelial proliferation, among others."}
{"id": "PMID:1100517", "title": "Distribution of cytophilic and anti-macrophage antibody on the macrophage surface.", "content": "The binding of cytophilic antibody to the macrophage surface results in patterns of distribution similar to those obtained when specific anti-macrophage antibody is used. These patterns are (a) diffuse circumferential binding at 4 degrees C, and (b) capping at 37 degrees C. The non-random distribution of the cytophilic antibody suggests that it binds to certain well-defined sites on the macrophage membrane.", "contents": "Distribution of cytophilic and anti-macrophage antibody on the macrophage surface. The binding of cytophilic antibody to the macrophage surface results in patterns of distribution similar to those obtained when specific anti-macrophage antibody is used. These patterns are (a) diffuse circumferential binding at 4 degrees C, and (b) capping at 37 degrees C. The non-random distribution of the cytophilic antibody suggests that it binds to certain well-defined sites on the macrophage membrane."}
{"id": "PMID:1100518", "title": "Endocytosis of chlorambucil-bound anti-tumor globulin following \"capping\" in EL4 lymphoma cells.", "content": "A rabbit 7 S antibody reacting specifically with a tumor assoicated antigen on the surface of mouse EL4 lymphoma cells could form \"caps\" and subsequently undergo endocytosis even when the antibody was non-covalently bound to chlorambucil. As the alkylation of nuclear DNA appears to be the basis of tumor inhibition by alkylating agents like chlorambucil, facilitation of the transport of chlorambucil across cell membrane by anti-tumor antibodies might explain, at least in part, the increased tumor inhibition by cholorambucil bound anti-tumor antibodies compared to tumor inhibition by equivalent amounts of tumor antibodies or chlorambucil alone.", "contents": "Endocytosis of chlorambucil-bound anti-tumor globulin following \"capping\" in EL4 lymphoma cells. A rabbit 7 S antibody reacting specifically with a tumor assoicated antigen on the surface of mouse EL4 lymphoma cells could form \"caps\" and subsequently undergo endocytosis even when the antibody was non-covalently bound to chlorambucil. As the alkylation of nuclear DNA appears to be the basis of tumor inhibition by alkylating agents like chlorambucil, facilitation of the transport of chlorambucil across cell membrane by anti-tumor antibodies might explain, at least in part, the increased tumor inhibition by cholorambucil bound anti-tumor antibodies compared to tumor inhibition by equivalent amounts of tumor antibodies or chlorambucil alone."}
{"id": "PMID:1100519", "title": "Studies on B- and T-cell receptors for lysozyme.", "content": "BALB/c mice have been immunized by intravenous administration of native or reduced and alkylated lysozyme. Primary immune response to these antigens was studied at the humoral level (by the Farr assay) and at the cellular level (by the rosette and the plaque assays using lysozyme coupled to sheep or pigeon erythrocytes). Antibodies and theta-negative RFC were specific for the antigen used for immunization. Specific inhibition of theta-negative RFC after incubation with the soluble immunizing antigen confirmed this specificity. Conversely, most theta-positive RFC had double specificity both to native and denatured lysozyme and showed lower avidity for the immunizing antigen, as shown by inhibition studies with soluble antigen. These data suggest that T cells have a broader specificity for lysozyme than B cells and also that, in this particular system, cytophilic antibodies are probably not responsible for the formation of theta-positive rosettes.", "contents": "Studies on B- and T-cell receptors for lysozyme. BALB/c mice have been immunized by intravenous administration of native or reduced and alkylated lysozyme. Primary immune response to these antigens was studied at the humoral level (by the Farr assay) and at the cellular level (by the rosette and the plaque assays using lysozyme coupled to sheep or pigeon erythrocytes). Antibodies and theta-negative RFC were specific for the antigen used for immunization. Specific inhibition of theta-negative RFC after incubation with the soluble immunizing antigen confirmed this specificity. Conversely, most theta-positive RFC had double specificity both to native and denatured lysozyme and showed lower avidity for the immunizing antigen, as shown by inhibition studies with soluble antigen. These data suggest that T cells have a broader specificity for lysozyme than B cells and also that, in this particular system, cytophilic antibodies are probably not responsible for the formation of theta-positive rosettes."}
{"id": "PMID:1100520", "title": "Age-dependent changes in the relative affinity of anti-dinitrophenyl antibodies in mice.", "content": "The affinity of anti-DNP antibodies produced by mice of various ages has been studied at the cellular level by the plaque inhibition technique. The affinity of PFC produced shortly after a single injection of antigen was found to increase during the first 6 weeks of life. The responses of 1- and 2-week-old animals also showed apparently restricted heterogeneity. The difference in the affinity of anti-DNP--PFC between young and adult mice could not be attributed to different (serum) levels of antigen or to differences in the rate of maturation of affinity during the immune response. Cell transfer experiments suggested that the age-dependent increase was due to a change in the population of antibody-forming (B) cell precursors and not to a progressive improvement of T-cell function. This findings is interpreted as favouring somatic mutation theories of antibody diversity.", "contents": "Age-dependent changes in the relative affinity of anti-dinitrophenyl antibodies in mice. The affinity of anti-DNP antibodies produced by mice of various ages has been studied at the cellular level by the plaque inhibition technique. The affinity of PFC produced shortly after a single injection of antigen was found to increase during the first 6 weeks of life. The responses of 1- and 2-week-old animals also showed apparently restricted heterogeneity. The difference in the affinity of anti-DNP--PFC between young and adult mice could not be attributed to different (serum) levels of antigen or to differences in the rate of maturation of affinity during the immune response. Cell transfer experiments suggested that the age-dependent increase was due to a change in the population of antibody-forming (B) cell precursors and not to a progressive improvement of T-cell function. This findings is interpreted as favouring somatic mutation theories of antibody diversity."}
{"id": "PMID:1100521", "title": "Experimental murine leprosy: growth of Mycobacterium lepraemurium in C3H and C57/BL mice after footpad inoculation.", "content": "Forty-three female C57/BL and C3H mice were inoculated with 2.7 X 10(6) Mycobacterium lepraemurium into each hind footpad. The foot thickness and the number of acid-fast bacilli in the footpad and popliteal and inquinal lymph nodes were recorded. In addition the morphological index and the mean bacillary length were determined in the footpad and in the popliteal lymph node. The bacilli multiplied in both strains during the first 4 weeks after inoculation. After that time no further increase in acid-fast bacilli was observed in the C57/BL strain; the bacilli became elongated and the morphological index decreased. These changes were preceded by a local swelling of the footpad due to the onset of an immune reaction. Thus, under the present conditions, C57/BL mice were able to resist experimental infection with M. lepraemurium by developing an immune response. In C3H mice no indication of an immune reaction was detected, and the bacilli continued to multiply throughout the observation period. The mouse footpad model seems to provide an excellent basis for the use of experimental murine leprosy to study immunity to mycobacterial infections. Certain aspects of the present model are discussed in relation to the mouse footpad model as used in the study of M. leprae infection in mice.", "contents": "Experimental murine leprosy: growth of Mycobacterium lepraemurium in C3H and C57/BL mice after footpad inoculation. Forty-three female C57/BL and C3H mice were inoculated with 2.7 X 10(6) Mycobacterium lepraemurium into each hind footpad. The foot thickness and the number of acid-fast bacilli in the footpad and popliteal and inquinal lymph nodes were recorded. In addition the morphological index and the mean bacillary length were determined in the footpad and in the popliteal lymph node. The bacilli multiplied in both strains during the first 4 weeks after inoculation. After that time no further increase in acid-fast bacilli was observed in the C57/BL strain; the bacilli became elongated and the morphological index decreased. These changes were preceded by a local swelling of the footpad due to the onset of an immune reaction. Thus, under the present conditions, C57/BL mice were able to resist experimental infection with M. lepraemurium by developing an immune response. In C3H mice no indication of an immune reaction was detected, and the bacilli continued to multiply throughout the observation period. The mouse footpad model seems to provide an excellent basis for the use of experimental murine leprosy to study immunity to mycobacterial infections. Certain aspects of the present model are discussed in relation to the mouse footpad model as used in the study of M. leprae infection in mice."}
{"id": "PMID:1100516", "title": "Induction of the immune response to cell surface antigens in vitro.", "content": "Two tissue culture incubation systems are described in which immune responses to cell surface antigens have been demonstrated. In the one-way \"mixed lymphocyte interaction\" system, a specific stimulation of thymidine uptake was induced by a particulate membrane antigen fraction, the microsomal lipoproteins (MLP), when low levels (0.01 to 0.001 mug per ml) were incubated with spleen or lymph node cells from nonsensitized mice. No stimulation was seen when allogeneic MLP was used at high levels, 10 mug per ml, nor at any level with syngeneic MLP. Specific effectors were demonstrated after 72-hr incubation with stimulatory levels of allogeneic MLP in three separate in vitro assays, a plaque-forming cell reduction assay, a tumor target assay, and an antigen-binding cell assay. In the latter assay, [125I]MLP was used as the source of antigen. This system has limited potential inasmuch as mouse spleen cells do not survive in it beyond the 4th day of culture. The second tissue culture system, the Marbrook system, has much greater possibilities because at least 25% of the inoculum is recovered 7 days later. In this culture system a cell-free sheep erythrocyte membrane preparation can induce plaque-forming cells in the absence of macrophages. Using a sensitive radioimmunoassay, free specific antibody was detected in culture supernatant fluids. With the same culture system, allogeneic lymphocytotoxic cells (killer) have been induced with spleen cells from unprimed mice in strains differing at the major histocompatibility locus (H-2). Allogeneic MLP induced very significant \"killer\" cell activity with spleen cells from primed mice. In a syngeneic tumor system, significant amounts of killer cell activity were induced with unprimed spleen cell inocula, and much larger amounts induced with spleen cells from immunized mice.", "contents": "Induction of the immune response to cell surface antigens in vitro. Two tissue culture incubation systems are described in which immune responses to cell surface antigens have been demonstrated. In the one-way \"mixed lymphocyte interaction\" system, a specific stimulation of thymidine uptake was induced by a particulate membrane antigen fraction, the microsomal lipoproteins (MLP), when low levels (0.01 to 0.001 mug per ml) were incubated with spleen or lymph node cells from nonsensitized mice. No stimulation was seen when allogeneic MLP was used at high levels, 10 mug per ml, nor at any level with syngeneic MLP. Specific effectors were demonstrated after 72-hr incubation with stimulatory levels of allogeneic MLP in three separate in vitro assays, a plaque-forming cell reduction assay, a tumor target assay, and an antigen-binding cell assay. In the latter assay, [125I]MLP was used as the source of antigen. This system has limited potential inasmuch as mouse spleen cells do not survive in it beyond the 4th day of culture. The second tissue culture system, the Marbrook system, has much greater possibilities because at least 25% of the inoculum is recovered 7 days later. In this culture system a cell-free sheep erythrocyte membrane preparation can induce plaque-forming cells in the absence of macrophages. Using a sensitive radioimmunoassay, free specific antibody was detected in culture supernatant fluids. With the same culture system, allogeneic lymphocytotoxic cells (killer) have been induced with spleen cells from unprimed mice in strains differing at the major histocompatibility locus (H-2). Allogeneic MLP induced very significant \"killer\" cell activity with spleen cells from primed mice. In a syngeneic tumor system, significant amounts of killer cell activity were induced with unprimed spleen cell inocula, and much larger amounts induced with spleen cells from immunized mice."}
{"id": "PMID:1100522", "title": "Comparison of leukocyte count and function in smoking and nonsmoking young men.", "content": "Leukocyte function and other hematological measurements were tested in 14 smoking and 13 nonsmoking young men free of intercurrent or chronic diseases. Leukocyte chemotaxis was depressed in smoking subjects when compared to the same subjects abstaining from cigarettes or to the nonsmokers. Smoking did not affect the whole blood bactericidal capacity of leukocytes and serum for Staphyloccus aureus or Klebsiella pneumoniae. Total leukocyte counts, hematocritis, and monocyte counts were higher in the smoking subjects when compared to the nonsmokers.", "contents": "Comparison of leukocyte count and function in smoking and nonsmoking young men. Leukocyte function and other hematological measurements were tested in 14 smoking and 13 nonsmoking young men free of intercurrent or chronic diseases. Leukocyte chemotaxis was depressed in smoking subjects when compared to the same subjects abstaining from cigarettes or to the nonsmokers. Smoking did not affect the whole blood bactericidal capacity of leukocytes and serum for Staphyloccus aureus or Klebsiella pneumoniae. Total leukocyte counts, hematocritis, and monocyte counts were higher in the smoking subjects when compared to the nonsmokers."}
{"id": "PMID:1100523", "title": "Klebsiella pneumoniae and Staphylococcus aureus infection in mice: difference in uremia and ammoniagenesis.", "content": "Lethal infections by Staphylococcus aureus and Klebsiella pneumoniae were compared for kidney-related effects in mice. K. pneumoniae caused uremia and an increase in blood ammonia that could reach 2.5 times normal. These events did not occur in mice inoculated with S. aureus. Use of germfree animals indicated that most of the increase in ammonia arose from the gut, presumably due to greater availability of urea and ureolysis. Injected ornithine restored blood ammonia to nearly normal levels and extended survival.", "contents": "Klebsiella pneumoniae and Staphylococcus aureus infection in mice: difference in uremia and ammoniagenesis. Lethal infections by Staphylococcus aureus and Klebsiella pneumoniae were compared for kidney-related effects in mice. K. pneumoniae caused uremia and an increase in blood ammonia that could reach 2.5 times normal. These events did not occur in mice inoculated with S. aureus. Use of germfree animals indicated that most of the increase in ammonia arose from the gut, presumably due to greater availability of urea and ureolysis. Injected ornithine restored blood ammonia to nearly normal levels and extended survival."}
{"id": "PMID:1100524", "title": "Model system for studying colonization and growth of bacteria on a hydroxyapatite surface.", "content": "A model system for the study of bacterial colonization and growth on a hydroxyapatite (HT) surface is described. Hydroxyapatite was crystallized over the surface of porous glass beads. Chemical analysis of the product showed that the ratio of Ca2+/P042- was indistinguishable from that of commercial HT powder. X-ray diffraction analysis supported the conclusion that the product was HT. A system employing [14C]polyethylene glycol, which selectively adsorbs to the glass surface of the beads, was developed to determine the amount of glass surface covered by HT. Over 90% of the glass surface could be covered by our method. The product, HT beads, consisted of approximately 20% (dry weight) HT. The HT beads possess several properties which make them potentially useful for studying microbial adherence, growth, and interactions. These include: (i) chemical similarity to the tooth surface, (ii) large surface area, and (iii) high density. We also describe a method for direct measurement of the microbial mass of cells growing on    beads. The method entails immobilizing a sample on a membrane filter (Millipore), staining it with amido black dye, and eluting the dye for spectrophotometric measurement. Streptococcus mutans served as the test organism. For free-growing bacteria the values measured with the filter assay were directly proportional to cell number, with a value of 1 mug of \"protein\" corresponding to about 1.5 X 10(6) colony-forming units, determined by viable count. For bacteria colonizing the beads, 1 mug of protein corresponded to about 2 X 10(7) colony-forming units on the beads during logarithmic growth. As the culture approached stationary phase, the efficiency of the assay decreased. These data indicate that multiple random samples, taken at a given time, are representative of the entire culture.", "contents": "Model system for studying colonization and growth of bacteria on a hydroxyapatite surface. A model system for the study of bacterial colonization and growth on a hydroxyapatite (HT) surface is described. Hydroxyapatite was crystallized over the surface of porous glass beads. Chemical analysis of the product showed that the ratio of Ca2+/P042- was indistinguishable from that of commercial HT powder. X-ray diffraction analysis supported the conclusion that the product was HT. A system employing [14C]polyethylene glycol, which selectively adsorbs to the glass surface of the beads, was developed to determine the amount of glass surface covered by HT. Over 90% of the glass surface could be covered by our method. The product, HT beads, consisted of approximately 20% (dry weight) HT. The HT beads possess several properties which make them potentially useful for studying microbial adherence, growth, and interactions. These include: (i) chemical similarity to the tooth surface, (ii) large surface area, and (iii) high density. We also describe a method for direct measurement of the microbial mass of cells growing on    beads. The method entails immobilizing a sample on a membrane filter (Millipore), staining it with amido black dye, and eluting the dye for spectrophotometric measurement. Streptococcus mutans served as the test organism. For free-growing bacteria the values measured with the filter assay were directly proportional to cell number, with a value of 1 mug of \"protein\" corresponding to about 1.5 X 10(6) colony-forming units, determined by viable count. For bacteria colonizing the beads, 1 mug of protein corresponded to about 2 X 10(7) colony-forming units on the beads during logarithmic growth. As the culture approached stationary phase, the efficiency of the assay decreased. These data indicate that multiple random samples, taken at a given time, are representative of the entire culture."}
{"id": "PMID:1100525", "title": "Hepatitis B antigens in serum and liver of chimpanzees acutely infected with hepatitis B virus.", "content": "We report the temporal patterns of various immunohistological and serological parameters of acute self-limited hepatitis B virus infection of two chimpanzees, and we provide evidence that the synthesis of hepatitis B core antigen precedes that of hepatitis B surface antigen. Our data suggest that existence of a biphasic hepatitis B virus infection involving a hematogenous reinfection of the liver and indicate that recruitment of liver cells to produce hepatitis B virus may occur in a pattern consistent with a replicative cycle of about 8 days.", "contents": "Hepatitis B antigens in serum and liver of chimpanzees acutely infected with hepatitis B virus. We report the temporal patterns of various immunohistological and serological parameters of acute self-limited hepatitis B virus infection of two chimpanzees, and we provide evidence that the synthesis of hepatitis B core antigen precedes that of hepatitis B surface antigen. Our data suggest that existence of a biphasic hepatitis B virus infection involving a hematogenous reinfection of the liver and indicate that recruitment of liver cells to produce hepatitis B virus may occur in a pattern consistent with a replicative cycle of about 8 days."}
{"id": "PMID:1100526", "title": "Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans.", "content": "An enterotoxin-producing strain of Escherichia coli isolated from a case of cholera-like diarrhea (E. coli strain H-10407) was found to possess a surface-associated colonization factor. Colonization was manifested as the ability of small inocula (10(5) bacteria) to attain large (10(9)) populations in the infant rabbit intestine with a concomitant diarrheal response. A laboratory-passed derivative of E. coli H-10407, designated H-10407-P, failed to exhibit an increase in population in the infant rabbit and also failed to induce diarrhea. Cell-free culture supernatant fluids of E. coli H-10407 and H-10407-P produced equivalent enterotoxic responses in infant and in adult rabbits. Specific anti-colonization factor antiserum was produced by adsorbing hyperimmune anti-H-10407 serum with both heat-killed and living cells E. coli H-10407-P. This specific adsorbed serum protected infant rabbits from challenge with living E. coli H-10407 although the serum did not possess bactericidal activity. The anti-colonization factor serum did not agglutinate a strain of E. coli K-12 possessing the K88 colonization factor peculiar to E. coli enterotoxigenic for swine. By electron microscopy it was demonstrated that E. coli H-10407, but not H10407-, possessed pilus-like surface structures which agglutinated with the specific adsorbed (anti-colonization factor) antiserum. E. coli H-10407 possessed three species of plasmid deoxyribonucleic acid, measuring 60 X 10(6), 42 X 10(6), and 3.7 X 10(6) daltons, respectively. E. coli H-10407-P possessed only the 42 X 10(6)- and the 3.7 X 10(6)-dalton plasmid species. Spontaneous loss of the specific H-10407 surface-associated antigen was accompanied by loss of the 60 X 10(6)-dalton species of plasmid deoxyribonucleic acid and loss of colonizing ability. Thus, it is concluded that the E. coli colonization factor described here is a virulence factor which may play an important and possibly essential role in naturally occurring E. coli enterotoxic diarrhea in man.", "contents": "Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. An enterotoxin-producing strain of Escherichia coli isolated from a case of cholera-like diarrhea (E. coli strain H-10407) was found to possess a surface-associated colonization factor. Colonization was manifested as the ability of small inocula (10(5) bacteria) to attain large (10(9)) populations in the infant rabbit intestine with a concomitant diarrheal response. A laboratory-passed derivative of E. coli H-10407, designated H-10407-P, failed to exhibit an increase in population in the infant rabbit and also failed to induce diarrhea. Cell-free culture supernatant fluids of E. coli H-10407 and H-10407-P produced equivalent enterotoxic responses in infant and in adult rabbits. Specific anti-colonization factor antiserum was produced by adsorbing hyperimmune anti-H-10407 serum with both heat-killed and living cells E. coli H-10407-P. This specific adsorbed serum protected infant rabbits from challenge with living E. coli H-10407 although the serum did not possess bactericidal activity. The anti-colonization factor serum did not agglutinate a strain of E. coli K-12 possessing the K88 colonization factor peculiar to E. coli enterotoxigenic for swine. By electron microscopy it was demonstrated that E. coli H-10407, but not H10407-, possessed pilus-like surface structures which agglutinated with the specific adsorbed (anti-colonization factor) antiserum. E. coli H-10407 possessed three species of plasmid deoxyribonucleic acid, measuring 60 X 10(6), 42 X 10(6), and 3.7 X 10(6) daltons, respectively. E. coli H-10407-P possessed only the 42 X 10(6)- and the 3.7 X 10(6)-dalton plasmid species. Spontaneous loss of the specific H-10407 surface-associated antigen was accompanied by loss of the 60 X 10(6)-dalton species of plasmid deoxyribonucleic acid and loss of colonizing ability. Thus, it is concluded that the E. coli colonization factor described here is a virulence factor which may play an important and possibly essential role in naturally occurring E. coli enterotoxic diarrhea in man."}
{"id": "PMID:1100527", "title": "Amount and avidity of antibody to Escherichia coli O antigen measured with the ammonium sulphate precipitation technique in children with urinary tract infections.", "content": "The antibody amounts and avidities were analyzed in 13 patients with acute primary pyelonephritis, 11 patients with acute primary cystitis, and one with ureterocele and recurrent infections, using the ammonium sulphate precipitation (ASP) technique. The ASP titrations did not discriminate as well between pyelonephritis and cystitis as do the determinations with the indirect hemagglutination technique. The increase of antibody titer and avidity detected by the ASP method in some of the cystitis patients suggested a deeper tissue involvement in some cases resulting in antibodies demonstrable with this technique. Since control patients also showed a somewhat heterogenous antibody response regarding titer and avidity it cannot be excluded that stimulation by Escherichia coli antigens in the gut is detected by the ASP method.", "contents": "Amount and avidity of antibody to Escherichia coli O antigen measured with the ammonium sulphate precipitation technique in children with urinary tract infections. The antibody amounts and avidities were analyzed in 13 patients with acute primary pyelonephritis, 11 patients with acute primary cystitis, and one with ureterocele and recurrent infections, using the ammonium sulphate precipitation (ASP) technique. The ASP titrations did not discriminate as well between pyelonephritis and cystitis as do the determinations with the indirect hemagglutination technique. The increase of antibody titer and avidity detected by the ASP method in some of the cystitis patients suggested a deeper tissue involvement in some cases resulting in antibodies demonstrable with this technique. Since control patients also showed a somewhat heterogenous antibody response regarding titer and avidity it cannot be excluded that stimulation by Escherichia coli antigens in the gut is detected by the ASP method."}
{"id": "PMID:1100528", "title": "Immunoglobulin-bearing lymphocytes: their demonstration in adult sheep and ontogeny in the sheep fetus.", "content": "A method for the preparation of lymphocytes from sheep blood is described. Lymphocytes from adult and fetal sheep have been examined by immunofluorescence for surface immunoglobulin (sIg) determinants. Young adults (1.5 years) had a mean of 23.2% sIg cells, older adults (8.75 years) a mean of 9.7% sIg cells. Pregnancy did not influence these values. The earliest lymphocytes with sIg in fetal lambs were demonstrable at 52 days (96 mm crown-rump length) and countable by 56 days (110 mm CRL) at 0.3% sIg. The percentage rose rapidly to 15.1% between 78 and 87 days, followed by a fall to 2.1% around 117 days with a second increase thereafter. The significance of this spontaneous appearance of fetal sIg cells is discussed.", "contents": "Immunoglobulin-bearing lymphocytes: their demonstration in adult sheep and ontogeny in the sheep fetus. A method for the preparation of lymphocytes from sheep blood is described. Lymphocytes from adult and fetal sheep have been examined by immunofluorescence for surface immunoglobulin (sIg) determinants. Young adults (1.5 years) had a mean of 23.2% sIg cells, older adults (8.75 years) a mean of 9.7% sIg cells. Pregnancy did not influence these values. The earliest lymphocytes with sIg in fetal lambs were demonstrable at 52 days (96 mm crown-rump length) and countable by 56 days (110 mm CRL) at 0.3% sIg. The percentage rose rapidly to 15.1% between 78 and 87 days, followed by a fall to 2.1% around 117 days with a second increase thereafter. The significance of this spontaneous appearance of fetal sIg cells is discussed."}
{"id": "PMID:1100529", "title": "[A system to aid decision-making. Application to automatic interpretation of vectorcardiograms].", "content": "This paper presents a complete and autonomous system for the automated diagnosis (heuristic approach). The system was worked out by means of a small computer. A flexible, evolutive, quasi-universal system is achieved through original procedures. A specialised language enables the users to describe diagnoses and their criteria in symbolic form. A suitable compiler translates this symbolic writing into an interpretable object program. This, with the Interpreter program, constitutes the 'Automated Diagnosis Program'. Our data are vectorcardiograms recorded according to the Frank orthogonal system. After an interactive pre-processing process, 180 parameters--mostly spatial--are computed. The data, the computed parameters and additional information are stored in a data bank. Finally, the medical interpretation is automatically selected from 125 possibilities. The user could also utilise a data bank interrogation language.", "contents": "[A system to aid decision-making. Application to automatic interpretation of vectorcardiograms]. This paper presents a complete and autonomous system for the automated diagnosis (heuristic approach). The system was worked out by means of a small computer. A flexible, evolutive, quasi-universal system is achieved through original procedures. A specialised language enables the users to describe diagnoses and their criteria in symbolic form. A suitable compiler translates this symbolic writing into an interpretable object program. This, with the Interpreter program, constitutes the 'Automated Diagnosis Program'. Our data are vectorcardiograms recorded according to the Frank orthogonal system. After an interactive pre-processing process, 180 parameters--mostly spatial--are computed. The data, the computed parameters and additional information are stored in a data bank. Finally, the medical interpretation is automatically selected from 125 possibilities. The user could also utilise a data bank interrogation language."}
{"id": "PMID:1100531", "title": "Attitudes toward alcohol and drug use and abuse. I. Demographic and correlational data.", "content": "Research concerning drug- and alcohol-related attitudes was reviewed. Demographic variables were not generally consistently related to attitudes. Most personality variables were also not consistently correlated with attitudes.", "contents": "Attitudes toward alcohol and drug use and abuse. I. Demographic and correlational data. Research concerning drug- and alcohol-related attitudes was reviewed. Demographic variables were not generally consistently related to attitudes. Most personality variables were also not consistently correlated with attitudes."}
{"id": "PMID:1100533", "title": "Chronic cerebral effects of alcohol and drug abuse.", "content": "A minority of alcohol abusers develop severe cerebral dysfunction in the form of Wernicke-Korsakoff syndrome. There is also evidence to suggest that cerebral dysfunction, particularly impaired abstracting ability, occurs in that larger population of heavy drinkers who do not go on to develop the  Wernicke-Korsakoff syndrome. There is no consistent evidence that long-term marijuana, hallucinogen, or sedative use causes lasting neuropsychological disturbance. The deficits in abstract thinking reported by some LSD studies are similar to deficits others have reported among alcoholics. Since the LSD studies were not controlled for alcohol use, their interpretation is difficult. It appears that cerebrovascular accidents occur more frequently and at a younger age among amphetamine abusers. There is no reliable information about possible other long-term effects of stimulants on the brain per se (i.e., nonvascular complications). Abuse of intravenous narcotics has been associated with case reports of transverse myelitis and encephalitis. It is not known whether this pathology is a direct or hypersensitivity effect of narcotic drugs, of adulterants, or of infection.", "contents": "Chronic cerebral effects of alcohol and drug abuse. A minority of alcohol abusers develop severe cerebral dysfunction in the form of Wernicke-Korsakoff syndrome. There is also evidence to suggest that cerebral dysfunction, particularly impaired abstracting ability, occurs in that larger population of heavy drinkers who do not go on to develop the  Wernicke-Korsakoff syndrome. There is no consistent evidence that long-term marijuana, hallucinogen, or sedative use causes lasting neuropsychological disturbance. The deficits in abstract thinking reported by some LSD studies are similar to deficits others have reported among alcoholics. Since the LSD studies were not controlled for alcohol use, their interpretation is difficult. It appears that cerebrovascular accidents occur more frequently and at a younger age among amphetamine abusers. There is no reliable information about possible other long-term effects of stimulants on the brain per se (i.e., nonvascular complications). Abuse of intravenous narcotics has been associated with case reports of transverse myelitis and encephalitis. It is not known whether this pathology is a direct or hypersensitivity effect of narcotic drugs, of adulterants, or of infection."}
{"id": "PMID:1100534", "title": "The use of tranquilizers in the treatment of mixed drug abuse.", "content": "If the abuse potential of tranquilizers, and particularly benzodiazepines, is examined by their administration as a therapeutic agent to persons dependent on alcohol, alcohol and drugs, or drugs alone, these substances are found to be potentially dependence producing with similar mechanisms to those of other hypnotics and sedatives. However, their abuse potential varies from one substance to another, and on the whole is relatively small. At all events, with constant medical supervision, patients who were previously dependent on a drug of barbiturate type do not independently increase their intake so as to become dependent on the tranquilizers. These experiments on persons with an inherent susceptibility to dependency show that tranquilizers must represent only a very small risk to the general population. The view that benzodiazepines are not potentially dependence producing has no more scientific foundation than the exaggerated fears of this potential. Important is that the uncontrolled supply of highly potent tranquilizers, and above all those with a strong sedative component, leads to dependence in a considerable percentage of cases in the treatment of mixed abuse. No conclusions in absolute terms on the risk presented by tranquilizers (and above all benzodiazepines) may be drawn from observations made in the uncontrolled administration of these substances in case of polytoxicomania. It follows that tranquilizers, which, as far as their therapeutic effect is concerned, are a valuable aid in the treatment of drug abuse, can be safely given when there is constant medical supervision combined with psychotherapy. The basic principle to be followed in the use of tranquilizers for the treatment of drug-dependent persons, is, therefore, their periodic administration in limited doses with constant medical supervision. In principle, however, because of the abuse potential which is nevertheless present, the same mode of administration is also to be recommended for other patient groups. Uncontrolled long-term administration is to be rejected in all circumstances, and supply should be strictly on prescription only in order to prevent uncontrolled acquisition. The various experiments carried out by us on the administration of benzodiazepines to patients with simple or mixed drug dependence show that the habit forming potential of benzodiazepines must, on the whole, be considered as being relatively small; at all events, this group of substances is the least hazardous of all known sedatives. This is of considerable importance, since the neuroleptics and antidepressants (which in themselves present no risk of abuse) are generally poorly tolerated and refused by patients with an inherent tendency towards drug dependence.", "contents": "The use of tranquilizers in the treatment of mixed drug abuse. If the abuse potential of tranquilizers, and particularly benzodiazepines, is examined by their administration as a therapeutic agent to persons dependent on alcohol, alcohol and drugs, or drugs alone, these substances are found to be potentially dependence producing with similar mechanisms to those of other hypnotics and sedatives. However, their abuse potential varies from one substance to another, and on the whole is relatively small. At all events, with constant medical supervision, patients who were previously dependent on a drug of barbiturate type do not independently increase their intake so as to become dependent on the tranquilizers. These experiments on persons with an inherent susceptibility to dependency show that tranquilizers must represent only a very small risk to the general population. The view that benzodiazepines are not potentially dependence producing has no more scientific foundation than the exaggerated fears of this potential. Important is that the uncontrolled supply of highly potent tranquilizers, and above all those with a strong sedative component, leads to dependence in a considerable percentage of cases in the treatment of mixed abuse. No conclusions in absolute terms on the risk presented by tranquilizers (and above all benzodiazepines) may be drawn from observations made in the uncontrolled administration of these substances in case of polytoxicomania. It follows that tranquilizers, which, as far as their therapeutic effect is concerned, are a valuable aid in the treatment of drug abuse, can be safely given when there is constant medical supervision combined with psychotherapy. The basic principle to be followed in the use of tranquilizers for the treatment of drug-dependent persons, is, therefore, their periodic administration in limited doses with constant medical supervision. In principle, however, because of the abuse potential which is nevertheless present, the same mode of administration is also to be recommended for other patient groups. Uncontrolled long-term administration is to be rejected in all circumstances, and supply should be strictly on prescription only in order to prevent uncontrolled acquisition. The various experiments carried out by us on the administration of benzodiazepines to patients with simple or mixed drug dependence show that the habit forming potential of benzodiazepines must, on the whole, be considered as being relatively small; at all events, this group of substances is the least hazardous of all known sedatives. This is of considerable importance, since the neuroleptics and antidepressants (which in themselves present no risk of abuse) are generally poorly tolerated and refused by patients with an inherent tendency towards drug dependence."}
{"id": "PMID:1100535", "title": "Effects of Demetrin on circulation.", "content": "In a double-blind study the influence of Demetrin on cardiovascular activity was investigated. Basic values before treatment were compared with values after 1 and 2 weeks' administration. The cardiovascular parameters \"pulse rate\" and \"blood pressure\" were studied in the orthostatic tolerance test (pivoting table) and in the bicycle ergometer test. Results were evaluated with variance analysis. Demetrin at dosage levels of 3 x 10 mg/day and 3 x 20 mg/day did not influence cardiovascular function. Occasionally mild sedation was observed.", "contents": "Effects of Demetrin on circulation. In a double-blind study the influence of Demetrin on cardiovascular activity was investigated. Basic values before treatment were compared with values after 1 and 2 weeks' administration. The cardiovascular parameters \"pulse rate\" and \"blood pressure\" were studied in the orthostatic tolerance test (pivoting table) and in the bicycle ergometer test. Results were evaluated with variance analysis. Demetrin at dosage levels of 3 x 10 mg/day and 3 x 20 mg/day did not influence cardiovascular function. Occasionally mild sedation was observed."}
{"id": "PMID:1100536", "title": "Dose titration of steroidal and non-steroidal topical anti-inflammatory agents.", "content": "Although there is a variety of animal models available, neither a single assay system nor the results of the various assays permit absolute protection of relative anti-inflammatory potency. Equally, current clinical dose titration studies, although more reliable, provide only gross estimates of therapeutic potency when conducted in certain clinical situations in a double-blind randomized fashion. This paper delineated clinical means to titrate more  objectively and accurately therapeutic potency in the patient. Moreover, it has submitted considerations as to how to assess therapeutic anti-inflammatory activity in the complex multi-component process of inflammation accompanying most dermatologic diseases that eventually may permit titration of specific anti-inflammatory compounds on certain tissue components of the inflammatory process.", "contents": "Dose titration of steroidal and non-steroidal topical anti-inflammatory agents. Although there is a variety of animal models available, neither a single assay system nor the results of the various assays permit absolute protection of relative anti-inflammatory potency. Equally, current clinical dose titration studies, although more reliable, provide only gross estimates of therapeutic potency when conducted in certain clinical situations in a double-blind randomized fashion. This paper delineated clinical means to titrate more  objectively and accurately therapeutic potency in the patient. Moreover, it has submitted considerations as to how to assess therapeutic anti-inflammatory activity in the complex multi-component process of inflammation accompanying most dermatologic diseases that eventually may permit titration of specific anti-inflammatory compounds on certain tissue components of the inflammatory process."}
{"id": "PMID:1100537", "title": "Assessment and treatment of abstinence in the infant of the drug-dependent mother.", "content": "To accomplish the assessment and treatment of abstinence in the infant of the drug dependent mother, a scoring system has been devised for use as a clinical and research tool. The score monitors the passively addicted infant in a more comprehensive and precise way than has previously been described, and permits us to be more objective in our clinical estimates of the abstinence syndrome. Further, this scoring system has been used by relating it to the dosage schedule of phenobarbital or paregoric as part of an ongoing research project designed to test the comparative usefulness of recommended treatments for neonates with abstinence symptoms. It has been found useful also in following the progression or diminution of symptomatology before, during and after drug therapy. Moreover, the scoring system provides a basis for developing uniform criteria for the assessment and treatment of the neonate of the addicted mother.", "contents": "Assessment and treatment of abstinence in the infant of the drug-dependent mother. To accomplish the assessment and treatment of abstinence in the infant of the drug dependent mother, a scoring system has been devised for use as a clinical and research tool. The score monitors the passively addicted infant in a more comprehensive and precise way than has previously been described, and permits us to be more objective in our clinical estimates of the abstinence syndrome. Further, this scoring system has been used by relating it to the dosage schedule of phenobarbital or paregoric as part of an ongoing research project designed to test the comparative usefulness of recommended treatments for neonates with abstinence symptoms. It has been found useful also in following the progression or diminution of symptomatology before, during and after drug therapy. Moreover, the scoring system provides a basis for developing uniform criteria for the assessment and treatment of the neonate of the addicted mother."}
{"id": "PMID:1100538", "title": "Carbenicillin in the first nine months of unrestricted use. A chapter in the natural history of an antibiotic.", "content": "During the first nine months following its release for general use, carbenicillin was administered to forty-two patients in Hahnemann Hospital. Thirty-nine available records of patients receiving the drug for other than urinary infection were reviewed. Among children, all but one had mucoviscidosis: most were given acceptable therapeutic regimens; survival rate was 80%, and their physicians were favorably impressed. Among adults, underlying disease was usually life threatening; most were inadequately treated; survival rate was 31%, and their physicians were not favorably impressed. The multiplicity of variables precludes objective evaluation of the drug under the circumstances of its use, but one that could have been avoided was the inconsistency of the therapeutic regimen, which in adult patients was too often insufficient. As used, therefore, carbenicillin has not provided the benefits predicted from its earlier controlled evaluation, and the large expenditure of money, particularly by adult patients, has been largely wasted.", "contents": "Carbenicillin in the first nine months of unrestricted use. A chapter in the natural history of an antibiotic. During the first nine months following its release for general use, carbenicillin was administered to forty-two patients in Hahnemann Hospital. Thirty-nine available records of patients receiving the drug for other than urinary infection were reviewed. Among children, all but one had mucoviscidosis: most were given acceptable therapeutic regimens; survival rate was 80%, and their physicians were favorably impressed. Among adults, underlying disease was usually life threatening; most were inadequately treated; survival rate was 31%, and their physicians were not favorably impressed. The multiplicity of variables precludes objective evaluation of the drug under the circumstances of its use, but one that could have been avoided was the inconsistency of the therapeutic regimen, which in adult patients was too often insufficient. As used, therefore, carbenicillin has not provided the benefits predicted from its earlier controlled evaluation, and the large expenditure of money, particularly by adult patients, has been largely wasted."}
{"id": "PMID:1100539", "title": "Prediction of psychotropic properties of lisuride hydrogen maleate by quantitative pharmaco-electroencephalogram.", "content": "Based on \"quantitative pharmaco-EEG\" using computer-analyzed EEG (CEEG) measurements, unknown CNS effects of lisuride hydrogen maleate (LHM) were established. CEEG profiles of LHM in low dosages (less than or equal to 10 mcg) are similar to CNS \"inhibitory\" compounds, while in higher dosages (25 mcg to 100 mcg) they resemble \"psychostimulant\" compounds. By measuring the brain function using computer period analysis of cerebral biopotentials, dose-efficacy relations were found (in the range of 25-75 mcg) which suggest the bioavailability of LHM at the CNS level. By comparing the CEEG profiles of LHM with the previously studied compounds, five different clinical uses of LHM were predicted. The pilot trials suggest that LHM may have therapeutic potentials in patients with \"aging\" and/or organic brain syndromes, and in children with behavioral disturbances.", "contents": "Prediction of psychotropic properties of lisuride hydrogen maleate by quantitative pharmaco-electroencephalogram. Based on \"quantitative pharmaco-EEG\" using computer-analyzed EEG (CEEG) measurements, unknown CNS effects of lisuride hydrogen maleate (LHM) were established. CEEG profiles of LHM in low dosages (less than or equal to 10 mcg) are similar to CNS \"inhibitory\" compounds, while in higher dosages (25 mcg to 100 mcg) they resemble \"psychostimulant\" compounds. By measuring the brain function using computer period analysis of cerebral biopotentials, dose-efficacy relations were found (in the range of 25-75 mcg) which suggest the bioavailability of LHM at the CNS level. By comparing the CEEG profiles of LHM with the previously studied compounds, five different clinical uses of LHM were predicted. The pilot trials suggest that LHM may have therapeutic potentials in patients with \"aging\" and/or organic brain syndromes, and in children with behavioral disturbances."}
{"id": "PMID:1100540", "title": "Clinical pharmacology of SQ 10,996, a potential antidepressive agent.", "content": "Single oral doses of SQ 10,996 ranging from 500 to 1000 mg (0.34 to 15.3 mg/kg), given once daily for 3 consecutive days to groups of healthy volunteers, were well tolerated. One of three subjects given 1250 mg (17.1 mg/kg) and two of three subjects given 1500 mg (15.6 and 21.4 mg/kg) became drowsy on the second and third days; this symptom disappeared within 24 hrs after cessation of dosage. A short-term, multiple-dose tolerance study was carried out with a formulation of SQ 10,996, the bioavailability of which was comparable to that of the formulation used earlier in the ascending-dose tolerance study. When 200-mg doses were administered every 12, 8, or 6 hrs over a 6-day period, mean steady-state serum concentrations of approximately 4, 7, and 8 mug/ml were attained within 48 hrs; unexpectedly, no subject showed any sign of drowsiness. The half-life for SQ 10,996 in serum, estimated from concentrations in serum after the last dose, was approximately 13 hrs, significantly shorter than the half-life found previously after the administration of single 10-mg doses. These clinical pharmacology studies in healthy volunteers have shown SQ 10,996 to be biologically available and well tolerated. Future studies will test its antidepressive potential in patients.", "contents": "Clinical pharmacology of SQ 10,996, a potential antidepressive agent. Single oral doses of SQ 10,996 ranging from 500 to 1000 mg (0.34 to 15.3 mg/kg), given once daily for 3 consecutive days to groups of healthy volunteers, were well tolerated. One of three subjects given 1250 mg (17.1 mg/kg) and two of three subjects given 1500 mg (15.6 and 21.4 mg/kg) became drowsy on the second and third days; this symptom disappeared within 24 hrs after cessation of dosage. A short-term, multiple-dose tolerance study was carried out with a formulation of SQ 10,996, the bioavailability of which was comparable to that of the formulation used earlier in the ascending-dose tolerance study. When 200-mg doses were administered every 12, 8, or 6 hrs over a 6-day period, mean steady-state serum concentrations of approximately 4, 7, and 8 mug/ml were attained within 48 hrs; unexpectedly, no subject showed any sign of drowsiness. The half-life for SQ 10,996 in serum, estimated from concentrations in serum after the last dose, was approximately 13 hrs, significantly shorter than the half-life found previously after the administration of single 10-mg doses. These clinical pharmacology studies in healthy volunteers have shown SQ 10,996 to be biologically available and well tolerated. Future studies will test its antidepressive potential in patients."}
{"id": "PMID:1100541", "title": "Serum growth hormone, serum immunoreactive insulin and blood glucose response to oral and intravenous diazepam in man.", "content": "The effect of diazepam on blood glucose, serum immunoreactive insulin (IRI), and growth hormone (GH) were studied in 10 volunteers who received diazepam in oral doses of 5 and 10 mg, and intravenously 10 mg. They also received placebo and saline treatment. There was a dose-dependent rise in GH after diazepam administration, and the rise was related to the peak plasma level of the drug. A highly significant correlation between the concentrations of serum GH and plasma diazepam was found. During the i.v. and oral administration of 10 mg of diazepam, the peak GH levels, reached in 30 and 60 minutes (19.6+/-2.9 and 15.2+/-3.2ng/ml, respectively), were significantly higher than those during saline and placebo periods (4.3+/-0.8 and 5.9+/-1.1 ng/ml, respectively). There was a tendency to a rise of blood glucose levels, but no significant changes of serum IRI.", "contents": "Serum growth hormone, serum immunoreactive insulin and blood glucose response to oral and intravenous diazepam in man. The effect of diazepam on blood glucose, serum immunoreactive insulin (IRI), and growth hormone (GH) were studied in 10 volunteers who received diazepam in oral doses of 5 and 10 mg, and intravenously 10 mg. They also received placebo and saline treatment. There was a dose-dependent rise in GH after diazepam administration, and the rise was related to the peak plasma level of the drug. A highly significant correlation between the concentrations of serum GH and plasma diazepam was found. During the i.v. and oral administration of 10 mg of diazepam, the peak GH levels, reached in 30 and 60 minutes (19.6+/-2.9 and 15.2+/-3.2ng/ml, respectively), were significantly higher than those during saline and placebo periods (4.3+/-0.8 and 5.9+/-1.1 ng/ml, respectively). There was a tendency to a rise of blood glucose levels, but no significant changes of serum IRI."}
{"id": "PMID:1100542", "title": "Tritiated digoxin: studies in renal disease in human subjects.", "content": "Digoxin is excreted primarily in the urine as the unchanged glycoside: 60-80% can be recovered from the urine in 7 days after a single intravenous dose in the human subject. Definition of the role of the kidney in digoxin excretion, turnover and metabolism was studied in 57 patients with renal disease, transplant candidates and/or donors and recipients of renal transplants. A single dose of 3H digoxin was given to the subjects, frequent serum samples were obtained and all urine and stools were saved for 7 days. All specimens were extracted with chloroform and digoxin, and its metabolites were separated by column chromatography. Results reveal that the serum T1/2 and the dominant T1/2 of digoxin are prolonged in renal disease in direct proportion to the reduction in creatinine clearance (r = 0.833). The blood urea nitrogen (BUN) is also related to digoxin clearance (r = 0.742). The higher the BUN, the less digoxin excreted in the urine. Anephric patients excrete more digoxin in stool, but this does not compensate for the lack of renal excretion. Transplanted kidneys excrete digoxin in proportion to renal functional capacity, as do patients who have experienced unilateral nephrectomy. Peritoneal or hemodialysis is not effective in removing digoxin from the human subject and may lead to digitalis intoxication if K+ is allowed to fall to critical levels. Digoxin excretion is not volume related, as patients with nephritogenic diabetes insipidus excrete the drug normally with urine volumes of 12 liters a day. Digoxin doses in renal insufficiency should be dictated by knowledge of renal functional ability of the kidneys and after \"normal\" loading doses, and maintenance doses should be 1/4 to 1/2 those usually administered.", "contents": "Tritiated digoxin: studies in renal disease in human subjects. Digoxin is excreted primarily in the urine as the unchanged glycoside: 60-80% can be recovered from the urine in 7 days after a single intravenous dose in the human subject. Definition of the role of the kidney in digoxin excretion, turnover and metabolism was studied in 57 patients with renal disease, transplant candidates and/or donors and recipients of renal transplants. A single dose of 3H digoxin was given to the subjects, frequent serum samples were obtained and all urine and stools were saved for 7 days. All specimens were extracted with chloroform and digoxin, and its metabolites were separated by column chromatography. Results reveal that the serum T1/2 and the dominant T1/2 of digoxin are prolonged in renal disease in direct proportion to the reduction in creatinine clearance (r = 0.833). The blood urea nitrogen (BUN) is also related to digoxin clearance (r = 0.742). The higher the BUN, the less digoxin excreted in the urine. Anephric patients excrete more digoxin in stool, but this does not compensate for the lack of renal excretion. Transplanted kidneys excrete digoxin in proportion to renal functional capacity, as do patients who have experienced unilateral nephrectomy. Peritoneal or hemodialysis is not effective in removing digoxin from the human subject and may lead to digitalis intoxication if K+ is allowed to fall to critical levels. Digoxin excretion is not volume related, as patients with nephritogenic diabetes insipidus excrete the drug normally with urine volumes of 12 liters a day. Digoxin doses in renal insufficiency should be dictated by knowledge of renal functional ability of the kidneys and after \"normal\" loading doses, and maintenance doses should be 1/4 to 1/2 those usually administered."}
{"id": "PMID:1100546", "title": "Methods of assessing gingival and periodontal disease: a review.", "content": "There has been an effort by the dental profession working in the field of gingival and periodontal disease to find a method of recording the extent and degree of pathological change in tissues leading from gingivitis to periodontitis and to measure reversible as well as irreversible changes. It is obvious that some form of index is required and it should have the following well-defined criteria: (1) Simplicity, (2) Accuracy, (3) Quantitativeness, (4) Reproducibility, (5) Speed, (6) Objectivity, and (7) Amenability to statistical analysis. Indices, as well as determining the prevalence of disease in the group under investigation at a given period in time, must also provide information on incidence of disease, i.e. at different periods of time. Indices must also give data that make it possible to verify the nature, severity and aetiology of the disease process and to evaluate therapeutic measures. Indices yield information about the success or failure of control and prevention of disease, affecting the gingivae and the periodontal tissues. A review of methods of assessing and recording gingival and periodontal disease is presented starting from the early investigations of the century. It is shown that subsequently many variations evolved, often with the same inherent difficulties of interpretation or application. Usually the initial stages of inflammation of the gingivae are more difficult to recognize than established disease. Present methods of recording as objectively and as quickly as possible the gingival state in population groups are discussed with emphasis upon the necessity for providing effective preventive measures based upon assessment of schoolchildren.", "contents": "Methods of assessing gingival and periodontal disease: a review. There has been an effort by the dental profession working in the field of gingival and periodontal disease to find a method of recording the extent and degree of pathological change in tissues leading from gingivitis to periodontitis and to measure reversible as well as irreversible changes. It is obvious that some form of index is required and it should have the following well-defined criteria: (1) Simplicity, (2) Accuracy, (3) Quantitativeness, (4) Reproducibility, (5) Speed, (6) Objectivity, and (7) Amenability to statistical analysis. Indices, as well as determining the prevalence of disease in the group under investigation at a given period in time, must also provide information on incidence of disease, i.e. at different periods of time. Indices must also give data that make it possible to verify the nature, severity and aetiology of the disease process and to evaluate therapeutic measures. Indices yield information about the success or failure of control and prevention of disease, affecting the gingivae and the periodontal tissues. A review of methods of assessing and recording gingival and periodontal disease is presented starting from the early investigations of the century. It is shown that subsequently many variations evolved, often with the same inherent difficulties of interpretation or application. Usually the initial stages of inflammation of the gingivae are more difficult to recognize than established disease. Present methods of recording as objectively and as quickly as possible the gingival state in population groups are discussed with emphasis upon the necessity for providing effective preventive measures based upon assessment of schoolchildren."}
{"id": "PMID:1100567", "title": "[Hair growth on pedunoular skin flaps in the guinea pig].", "content": "In animal experiments with 12 male guinea pigs skin defects have been covered with one-sided bridge flaps. As opposed to corresponding earlier investigations on free full thickness skin autografts no irreversible and much less reversible damage to the hair follicles could be demonstrated in these skin flaps in the primary postoperative phase. In the later postoperative phase, there was--similar to the free full thickness skin autografts--a synchronization of hair change over several cycles, but on the other hand there was no precipitation of hair change. As a result of these findings it may be assumed that the secondary damage to the hair follicles in the later postoperative phase is not as pronounced in these flaps as in free full thickness skin autografts.", "contents": "[Hair growth on pedunoular skin flaps in the guinea pig]. In animal experiments with 12 male guinea pigs skin defects have been covered with one-sided bridge flaps. As opposed to corresponding earlier investigations on free full thickness skin autografts no irreversible and much less reversible damage to the hair follicles could be demonstrated in these skin flaps in the primary postoperative phase. In the later postoperative phase, there was--similar to the free full thickness skin autografts--a synchronization of hair change over several cycles, but on the other hand there was no precipitation of hair change. As a result of these findings it may be assumed that the secondary damage to the hair follicles in the later postoperative phase is not as pronounced in these flaps as in free full thickness skin autografts."}
{"id": "PMID:1100568", "title": "[Acquired epidermolysis bullosa in Crohn's disease].", "content": "A case of epidermolysis bullosa acquisita associated with colitis granulomatosa (Crohn's disease) is reported. Although the cutaneous lesions of this unusual disease resemble in their clinical, histological and ultrastructural features to those of the hereditary epidermolysis bullosa dystrophica, the epidermolysis bullosa acquisita has to be regarded as a separate entity among the blistering epidermolysis because of its late manifestation in adults without evidence of hereditary disorders. The deposits of immunoglobulins in the involved skin of the acral areas demonstrated fluorescentmicroscopically are discussed in regard of a possible etiopathogenic relation with the primary irritated intestinal disease.", "contents": "[Acquired epidermolysis bullosa in Crohn's disease]. A case of epidermolysis bullosa acquisita associated with colitis granulomatosa (Crohn's disease) is reported. Although the cutaneous lesions of this unusual disease resemble in their clinical, histological and ultrastructural features to those of the hereditary epidermolysis bullosa dystrophica, the epidermolysis bullosa acquisita has to be regarded as a separate entity among the blistering epidermolysis because of its late manifestation in adults without evidence of hereditary disorders. The deposits of immunoglobulins in the involved skin of the acral areas demonstrated fluorescentmicroscopically are discussed in regard of a possible etiopathogenic relation with the primary irritated intestinal disease."}
{"id": "PMID:1100573", "title": "Enzyme cytochemistry of blood and marrow cells.", "content": "Diseases of the blood and bone marrow are commonly associated with abnormalities of oxido-reductase and lysosomal enzymes within individual erythrocytes and leucocytes. There are considerable technical difficulties, however, in adapting enzyme histochemical techniques to the study of haemopoietic tissue since individual cells are readily disrupted during processing, show variable enzyme activity according to the stage of maturation, and possess a lipoprotein cytoplasmic membrane which hinders reagent penetration. Cytochemical techniques for the study of oxido-reductase systems are of importance in the study of the neutrophil in infected patients, the erythrocyte in glucose-6-phosphate dehydrogenase deficiency, and the primitive blast cell in acute leukaemia. Lysosomal enzymes are of importance in the study of the neutrophil in infected patients and in the differential diagnosis of acute leukaemia. Some examples of recent studies of these enzyme systems are given to illustrate technical procedures involving cytocentrifugation of cells on to glass slides, adjustment of the osmolality of the reaction mixture, and the study of smeared cells as opposed to cells incubated in suspension.", "contents": "Enzyme cytochemistry of blood and marrow cells. Diseases of the blood and bone marrow are commonly associated with abnormalities of oxido-reductase and lysosomal enzymes within individual erythrocytes and leucocytes. There are considerable technical difficulties, however, in adapting enzyme histochemical techniques to the study of haemopoietic tissue since individual cells are readily disrupted during processing, show variable enzyme activity according to the stage of maturation, and possess a lipoprotein cytoplasmic membrane which hinders reagent penetration. Cytochemical techniques for the study of oxido-reductase systems are of importance in the study of the neutrophil in infected patients, the erythrocyte in glucose-6-phosphate dehydrogenase deficiency, and the primitive blast cell in acute leukaemia. Lysosomal enzymes are of importance in the study of the neutrophil in infected patients and in the differential diagnosis of acute leukaemia. Some examples of recent studies of these enzyme systems are given to illustrate technical procedures involving cytocentrifugation of cells on to glass slides, adjustment of the osmolality of the reaction mixture, and the study of smeared cells as opposed to cells incubated in suspension."}
{"id": "PMID:1100577", "title": "Retention of Salmonella typhimurium by certain species of fish and shrimp.", "content": "Marine fish (Mugil cephalus, Fundulus heteroclitus, Trachinotus carolinus), lake shrimp (Penaeus setiferus), and channel catfish (Ictalurus lacustris punctatus) were exposed to Salmonella typhimurium and cultured after 2 hours and at daily intervals for 30 days. Salmonella typhimurium was recovered from all exposed animals after 2 hours and for 30 days in all animals except P setifurus. The organisms could not be recovered from those shrimp after the initial sampling period. Apparent salmonellosis developed in certain specimens of M cephalus and T carolinus 10 to 14 days after exposure to S typhimurium.", "contents": "Retention of Salmonella typhimurium by certain species of fish and shrimp. Marine fish (Mugil cephalus, Fundulus heteroclitus, Trachinotus carolinus), lake shrimp (Penaeus setiferus), and channel catfish (Ictalurus lacustris punctatus) were exposed to Salmonella typhimurium and cultured after 2 hours and at daily intervals for 30 days. Salmonella typhimurium was recovered from all exposed animals after 2 hours and for 30 days in all animals except P setifurus. The organisms could not be recovered from those shrimp after the initial sampling period. Apparent salmonellosis developed in certain specimens of M cephalus and T carolinus 10 to 14 days after exposure to S typhimurium."}
{"id": "PMID:1100593", "title": "Postnatal development of pyrogenic sensitivity in guinea pigs.", "content": "Despite evidence of thermoregulatory ability from birth, neonates generally are unable to develop fever when challenged with endotoxin. This could be due to their small capacity for heat storage. To test this possibility, the pyrogenicity of S. enteritidis endotoxin (2 mug/kg, iv) was measured at both room (Ta = 25 degrees C) and neutral (Tn = 29-33 degrees C, depending on age) temperatures in 0- to 32-day-old unanesthetized guinea pigs, reared from birth at about 24 degrees C. Control guinea pigs received sterile saline injections in concurrent experiments. Shivering, O2 uptake, and colonic (Tre) and subcutaneous [over the interscapular fat pad (Tbat) and the sacrospinalis muscle (Tsc)] temperatures were recorded continuously for 4 h after injection. Endotoxin generally produced no febrile responses at both ambient temperaturess  rises in animals aged 8 or more days; Tbat increased before the other sites in the 8- and 16-day-old animals, and shivering did not occur; by 32 days of age, however, Tbat no longer increased first, and there was shivering. In Tn significant febrile rises were not evident until 32 days of age; control temperatures, however, were elevated during this exposure as compared to at Ta. These results showed therefore that pyrogenic sensitivity is not apparent in guinea pigs during the first postnatal week; thereafter fever responses are evocable, but their detection may be masked by environmentally produced changes in body temperature. The data also indicated that the site of the heat production underlying, in part, endotoxic fevers gradually shifts from brown fat so skeletal muscle during the first month of life.", "contents": "Postnatal development of pyrogenic sensitivity in guinea pigs. Despite evidence of thermoregulatory ability from birth, neonates generally are unable to develop fever when challenged with endotoxin. This could be due to their small capacity for heat storage. To test this possibility, the pyrogenicity of S. enteritidis endotoxin (2 mug/kg, iv) was measured at both room (Ta = 25 degrees C) and neutral (Tn = 29-33 degrees C, depending on age) temperatures in 0- to 32-day-old unanesthetized guinea pigs, reared from birth at about 24 degrees C. Control guinea pigs received sterile saline injections in concurrent experiments. Shivering, O2 uptake, and colonic (Tre) and subcutaneous [over the interscapular fat pad (Tbat) and the sacrospinalis muscle (Tsc)] temperatures were recorded continuously for 4 h after injection. Endotoxin generally produced no febrile responses at both ambient temperaturess  rises in animals aged 8 or more days; Tbat increased before the other sites in the 8- and 16-day-old animals, and shivering did not occur; by 32 days of age, however, Tbat no longer increased first, and there was shivering. In Tn significant febrile rises were not evident until 32 days of age; control temperatures, however, were elevated during this exposure as compared to at Ta. These results showed therefore that pyrogenic sensitivity is not apparent in guinea pigs during the first postnatal week; thereafter fever responses are evocable, but their detection may be masked by environmentally produced changes in body temperature. The data also indicated that the site of the heat production underlying, in part, endotoxic fevers gradually shifts from brown fat so skeletal muscle during the first month of life."}
{"id": "PMID:1100594", "title": "Stability in lobar ventilation distribution during change in thoracic configuration.", "content": "Stability in lobar ventilation was examined in dogs during bilateral electrophrenic respiration (BEPR) and positive pressure-assisted ventilation (PPAV). Bilateral prior ligation of the lower and middle lobe pulmonary arteries monitoring of upper lobe ventilation as alveolar minute ventilation (VA), middle and lower lobe ventilation as dead space (VD), and VD/VT ratio, both calculated by the Bohr equation. As documented by chest films, transverse and anteroposterior thoracic diameters during BEPR decreased below FRC values whereas thoracic cephalocaudal dimension greatly increased. During PPAV, all thoracic dimensions increased. Despite these dissimilar regional chest movements, VA, VD, and VD/VT ratio were comparable between PPAV and BEPR under conditions of matched tidal volume and respiratory frequency. Stability in upper lobe ventilation during BEPR was maintained by caudal displacement despite the compression of the rib cage, as documented by tantalum bronchography. Lobar-interdependence appears to be the mechanism transmitting negative pleural pressure developed by the diaphragm to the upper lobes via lower and middle lobe inflation.", "contents": "Stability in lobar ventilation distribution during change in thoracic configuration. Stability in lobar ventilation was examined in dogs during bilateral electrophrenic respiration (BEPR) and positive pressure-assisted ventilation (PPAV). Bilateral prior ligation of the lower and middle lobe pulmonary arteries monitoring of upper lobe ventilation as alveolar minute ventilation (VA), middle and lower lobe ventilation as dead space (VD), and VD/VT ratio, both calculated by the Bohr equation. As documented by chest films, transverse and anteroposterior thoracic diameters during BEPR decreased below FRC values whereas thoracic cephalocaudal dimension greatly increased. During PPAV, all thoracic dimensions increased. Despite these dissimilar regional chest movements, VA, VD, and VD/VT ratio were comparable between PPAV and BEPR under conditions of matched tidal volume and respiratory frequency. Stability in upper lobe ventilation during BEPR was maintained by caudal displacement despite the compression of the rib cage, as documented by tantalum bronchography. Lobar-interdependence appears to be the mechanism transmitting negative pleural pressure developed by the diaphragm to the upper lobes via lower and middle lobe inflation."}
{"id": "PMID:1100595", "title": "Determination of plasma volume by 113min-labeled transferrin.", "content": "The binding property of ionic indium (In3+) with plasma transferrin was utilized for determination of plasma volumes (PV) of whole body and individual organs in small animals. Plasma transferrin from a donor rat was labeled with 15-17 muCi 113mIn/ml plasma and injected into the tested rats. PV were determined either by extrapolation to the dilution at time zero (for whole animals) or by calculation of the ratio, organ radiation: radiation of a plasma unit volume (for organs). The reliability of the method for determination of whole-body PV was ascertained by comparing the results obtained with those obtained simultaneously by the Evans blue dilution method. Whole body PV values obtained by the two methods were similar, with a correlation coefficient (r) of 0.997. The short half life of 113mIn enables it to be used with other nuclides which have similar or different energies in the same sample; indium radiation was counted first and after it had disappeared the activity of the other nuclide could be measured.", "contents": "Determination of plasma volume by 113min-labeled transferrin. The binding property of ionic indium (In3+) with plasma transferrin was utilized for determination of plasma volumes (PV) of whole body and individual organs in small animals. Plasma transferrin from a donor rat was labeled with 15-17 muCi 113mIn/ml plasma and injected into the tested rats. PV were determined either by extrapolation to the dilution at time zero (for whole animals) or by calculation of the ratio, organ radiation: radiation of a plasma unit volume (for organs). The reliability of the method for determination of whole-body PV was ascertained by comparing the results obtained with those obtained simultaneously by the Evans blue dilution method. Whole body PV values obtained by the two methods were similar, with a correlation coefficient (r) of 0.997. The short half life of 113mIn enables it to be used with other nuclides which have similar or different energies in the same sample; indium radiation was counted first and after it had disappeared the activity of the other nuclide could be measured."}
{"id": "PMID:1100596", "title": "Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor.", "content": "Mutants affected in lamB, the structural gene for phage lambda receptor, are unable to utilize maltose when it is present at low concentrations (less than or equal 10 muM). During growth in a chemostat at limiting maltose concentrations, the lamB mutants tested were selected against in the presence of the wild-type strain. Transport studies demonstrate that most lamB mutants have deficient maltose transport capacities at low maltose concentrations. When antibodies against purified phage lambda receptor are added to a wild-type strain, transport of maltose at low concentrations is significantly reduced. These results strongly suggest that the phage lambda receptor molecule is involved in maltose transport.", "contents": "Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor. Mutants affected in lamB, the structural gene for phage lambda receptor, are unable to utilize maltose when it is present at low concentrations (less than or equal 10 muM). During growth in a chemostat at limiting maltose concentrations, the lamB mutants tested were selected against in the presence of the wild-type strain. Transport studies demonstrate that most lamB mutants have deficient maltose transport capacities at low maltose concentrations. When antibodies against purified phage lambda receptor are added to a wild-type strain, transport of maltose at low concentrations is significantly reduced. These results strongly suggest that the phage lambda receptor molecule is involved in maltose transport."}
{"id": "PMID:1100597", "title": "Role of the receptor for bacteriophage lambda in the functioning of the maltose chemoreceptor of Escherichia coli.", "content": "Chemotaxis towards maltose is specifically defective in many strains of Escherichia coli carrying mutations affecting lamB, the gene coding for the outer membrane receptor for bacteriophage lambda. However, with one exception, the most extreme effect of lamB mutants on the maltose response as determined in the capillary assay is a shift to higher sugar concentrations and a reduction in the number of bacteria accumulated to about 25% of the wild-type level. The severity of the taxis defect is strongly correlated with reduced ability of the cells to take up the maltose present at 1 and 10 muM. Evidence presented here and in the accompanying paper indicates that the lambda receptor is involved in the transport of maltose at these concentrations. The effects of lamB mutations on maltose taxis can be explained by postulating that the high-affinity maltose transport system in which the lambda receptor participates transfers maltose from the surrounding medium across the outer membrane and into the periplasmic space. If the maltose chemoreceptor detects sugar present in the periplasmic space, and not molecules external to the outer membrane, then defective transport of low concentrations of maltose into the periplasm would result in the observed apparent reduction in the sensitivity of the maltose receptor. Thus, the lambda receptor protein would participate in maltose chemorecepton only indirectly through its role in maltose transport.", "contents": "Role of the receptor for bacteriophage lambda in the functioning of the maltose chemoreceptor of Escherichia coli. Chemotaxis towards maltose is specifically defective in many strains of Escherichia coli carrying mutations affecting lamB, the gene coding for the outer membrane receptor for bacteriophage lambda. However, with one exception, the most extreme effect of lamB mutants on the maltose response as determined in the capillary assay is a shift to higher sugar concentrations and a reduction in the number of bacteria accumulated to about 25% of the wild-type level. The severity of the taxis defect is strongly correlated with reduced ability of the cells to take up the maltose present at 1 and 10 muM. Evidence presented here and in the accompanying paper indicates that the lambda receptor is involved in the transport of maltose at these concentrations. The effects of lamB mutations on maltose taxis can be explained by postulating that the high-affinity maltose transport system in which the lambda receptor participates transfers maltose from the surrounding medium across the outer membrane and into the periplasmic space. If the maltose chemoreceptor detects sugar present in the periplasmic space, and not molecules external to the outer membrane, then defective transport of low concentrations of maltose into the periplasm would result in the observed apparent reduction in the sensitivity of the maltose receptor. Thus, the lambda receptor protein would participate in maltose chemorecepton only indirectly through its role in maltose transport."}
{"id": "PMID:1100598", "title": "Isolation of an Escherichia coli mutant deficient in glutathione synthesis.", "content": "A mutant of Escherichia coli that contains essentially no detectable glutathione has been isolated. The mutant contains a very low level of the enzyme glutathione synthetase and accumulates lambda-glutamyl cysteine at a concentration approximately equal to the level of glutathione found in its parent. No significant differences in growth were observed between the mutant and its parent. However, the activity of at least one enzyme was found to be affected by the absence of glutathione; the specific activity of the B1 subunit of ribonucleoside diphosphate reductase was greatly reduced. The possibility that the decreased B1 activity is due to a mutation in the structural gene coding for B1 or its regulatory gene could be eliminated. This suggests that one role of glutathione in the cell is to maintain at least this one protein in an active state. We propose the designation gshB for the gene coding for glutathione synthetase.", "contents": "Isolation of an Escherichia coli mutant deficient in glutathione synthesis. A mutant of Escherichia coli that contains essentially no detectable glutathione has been isolated. The mutant contains a very low level of the enzyme glutathione synthetase and accumulates lambda-glutamyl cysteine at a concentration approximately equal to the level of glutathione found in its parent. No significant differences in growth were observed between the mutant and its parent. However, the activity of at least one enzyme was found to be affected by the absence of glutathione; the specific activity of the B1 subunit of ribonucleoside diphosphate reductase was greatly reduced. The possibility that the decreased B1 activity is due to a mutation in the structural gene coding for B1 or its regulatory gene could be eliminated. This suggests that one role of glutathione in the cell is to maintain at least this one protein in an active state. We propose the designation gshB for the gene coding for glutathione synthetase."}
{"id": "PMID:1100599", "title": "Escherichia coli deoxyribonucleic acid synthesis mutants: their effect upon bacteriophage P2 and satellite bacteriophage P4 deoxyribonucleic acid synthesis.", "content": "Escherichia coli C strains containing different deoxyribonucleic acid (DNA) synthesis mutations have been tested for their support of the DNA synthesis of bacteriophage P2 and its satellite phage P4. Bacteriophage P2 requires functional dnaB, dnaE, and dnaG E. coli gene products for DNA synthesis, whereas it does not require the products of the dnaA, dnaC, or dnaH genes. In contrast, the satellite virus P4 requires functional dnaE and dnaH gene products for DNA synthesis and does not need the products of the dnaA, dnaB, dnaC, and dnaG genes. Thus the P2 and P4 genomes are replicated differently, even though they are packaged in heads made of the same protein.", "contents": "Escherichia coli deoxyribonucleic acid synthesis mutants: their effect upon bacteriophage P2 and satellite bacteriophage P4 deoxyribonucleic acid synthesis. Escherichia coli C strains containing different deoxyribonucleic acid (DNA) synthesis mutations have been tested for their support of the DNA synthesis of bacteriophage P2 and its satellite phage P4. Bacteriophage P2 requires functional dnaB, dnaE, and dnaG E. coli gene products for DNA synthesis, whereas it does not require the products of the dnaA, dnaC, or dnaH genes. In contrast, the satellite virus P4 requires functional dnaE and dnaH gene products for DNA synthesis and does not need the products of the dnaA, dnaB, dnaC, and dnaG genes. Thus the P2 and P4 genomes are replicated differently, even though they are packaged in heads made of the same protein."}
{"id": "PMID:1100600", "title": "Indirect selection of bacterial plasmids lacking identifiable phenotypic properties.", "content": "A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasmid also contain the nonselected plasmid. A temperature-sensitive mutation in the replication functions of the indicator plasmid enables its elimination from doubly transformed bacteria. Using this procedure, we have isolated bacteria that carry only the small cryptic plasmid. P15A, of the Escherichia coli strain 15. This genetic element, which contains only 2,300 nucleotide pairs, is thus capable of functioning as a replicon independently of the two larger plasmids normally associated with it in E. coli 15 strains (Ikeda, Inuzuka, and Tomizawa, 1970).", "contents": "Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasmid also contain the nonselected plasmid. A temperature-sensitive mutation in the replication functions of the indicator plasmid enables its elimination from doubly transformed bacteria. Using this procedure, we have isolated bacteria that carry only the small cryptic plasmid. P15A, of the Escherichia coli strain 15. This genetic element, which contains only 2,300 nucleotide pairs, is thus capable of functioning as a replicon independently of the two larger plasmids normally associated with it in E. coli 15 strains (Ikeda, Inuzuka, and Tomizawa, 1970)."}
{"id": "PMID:1100601", "title": "Growth rate of Enterobacteriaceae at elevated temperatures: limitation by methionine.", "content": "The effect of elevated temperatures on growth rate was studied in five strains of Enterobacteriaceae. In all the strains tested a shift to the elevated temperature resulted in an immediate decrease in growth rate which was due to limitation in the availability of endogenous methionine. The first biosynthetic enzyme of the methionine pathway-homoserine transsuccinylase-was studied in extracts of Aerobacter aerogenes, Salmonella typhimurium, and Escherichia coli and was shown to be temperature sensitive in all of them.", "contents": "Growth rate of Enterobacteriaceae at elevated temperatures: limitation by methionine. The effect of elevated temperatures on growth rate was studied in five strains of Enterobacteriaceae. In all the strains tested a shift to the elevated temperature resulted in an immediate decrease in growth rate which was due to limitation in the availability of endogenous methionine. The first biosynthetic enzyme of the methionine pathway-homoserine transsuccinylase-was studied in extracts of Aerobacter aerogenes, Salmonella typhimurium, and Escherichia coli and was shown to be temperature sensitive in all of them."}
{"id": "PMID:1100603", "title": "Role of methionine in the regulation of serine hydroxymethyltransferase in Eschericia coli.", "content": "Significant derepression of serine hydroxymethyltransferase is observed when metE or metF mutants of Escherichia coli K-12 are grown on D-methionine sulfoxide instead of L-methionine. The derepression is not prevented by addition of glycine, adenosine, guanosine, guanosine, and thymidine to the growth medium of methionine-limited metF cells showing that the effect is not due to a secondary deficiency of these nutrients. On the other hand, methionine-limited growth of a metA mutant leads to derepression of met regulon enzymes, but only a marginal increase in serine hydroxymethyltransferase activity. A prototrophic metJ strain grown on minimal medium has about the same serine hydroxymethyltransferase as the wild type. The enzyme activity of the metJ strain is not influenced by methionine, but it is partially repressed by glycine, adenosine, and thymidine. metK strains have about twice as much serine hydroxymethyltransferase activity as wild-type cells when grown on minimal medium; but when both types of cells are grown on medium supplemented with glycine, adenosine, guanosine, and thymidine, their enzyme activities are about the same. The results show that methionine limitation can lead to depression of serine hydroxymethyltransferase, but that the regulatory system is different from the one which controls the methionine regulon.", "contents": "Role of methionine in the regulation of serine hydroxymethyltransferase in Eschericia coli. Significant derepression of serine hydroxymethyltransferase is observed when metE or metF mutants of Escherichia coli K-12 are grown on D-methionine sulfoxide instead of L-methionine. The derepression is not prevented by addition of glycine, adenosine, guanosine, guanosine, and thymidine to the growth medium of methionine-limited metF cells showing that the effect is not due to a secondary deficiency of these nutrients. On the other hand, methionine-limited growth of a metA mutant leads to derepression of met regulon enzymes, but only a marginal increase in serine hydroxymethyltransferase activity. A prototrophic metJ strain grown on minimal medium has about the same serine hydroxymethyltransferase as the wild type. The enzyme activity of the metJ strain is not influenced by methionine, but it is partially repressed by glycine, adenosine, and thymidine. metK strains have about twice as much serine hydroxymethyltransferase activity as wild-type cells when grown on minimal medium; but when both types of cells are grown on medium supplemented with glycine, adenosine, guanosine, and thymidine, their enzyme activities are about the same. The results show that methionine limitation can lead to depression of serine hydroxymethyltransferase, but that the regulatory system is different from the one which controls the methionine regulon."}
{"id": "PMID:1100602", "title": "Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.", "content": "Mutants of Escherichia coli K-12 unable to grow on any of the three naturally occurring hexitols D-manitol, D-glucitol, and galactitol and, among these specifically, mutants with altered transport and phosphorylating activity have been isolated. Different isolation procedures have been utilized, including suicide by D-[3H]mannitol, chemotaxis, and resistance to the toxic hexitol analogue 2-deoxy-arabino-hexitol. Mutations thus obtained have been mapped in four distinct operons. (i) Mutations affecting an enzyme II-complexmt1 activity of the phosphoenolpyruvate-dependent phosphotransferase system all map in gene mtlA. This gene has previously been shown (Solomon and Lin, 1972) to be part of an operon, mtl, located at 71 min on the E. coli linkage map containing, in addition to mtlA, the cis-dominant regulatory gene mtlC and mtlD, the structural gene for the enzyme D-mannitol-1-phosphate dehydrogenase. The gene order in this operon, induced by D-mannitol, is mtlC A D. (ii) Mutations in gene gutA affecting a second enzyme II-complexgut of the phosphotransferase system map at 51 min, clustered in operon gutC A D together with the cis-dominant regulatory gene gutC and the structural gene gutD for the enzyme D-glucitol-6-phosphate dehydrogenase. The gut operon, previously called sbl or srl, is induced by D-glucitol. (iii) Mutations affecting the transport and catabolism of galactitol are clustered in a third operon, gatC A D, located at 40.5 min. This operon again contains a cis-dominant regulatory gene, gatC, the structural gene gatD for galactitol-1-phosphate dehydrogenase, and gene gatA coding for a thrid hexitol-specific enzyme II-complexgat. Other genes coding for two additional enzymes involved in galactitol catabolism apparently are not linked to gatC A D. (iv) A fourth class of mutants pleiotropically negative for hexitol growth and transport maps in the pts operon. Triple-negative mutants (mtlA gutA gatA) do not have further transport or phosphorylating activity for any of the three hexitols.", "contents": "Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping. Mutants of Escherichia coli K-12 unable to grow on any of the three naturally occurring hexitols D-manitol, D-glucitol, and galactitol and, among these specifically, mutants with altered transport and phosphorylating activity have been isolated. Different isolation procedures have been utilized, including suicide by D-[3H]mannitol, chemotaxis, and resistance to the toxic hexitol analogue 2-deoxy-arabino-hexitol. Mutations thus obtained have been mapped in four distinct operons. (i) Mutations affecting an enzyme II-complexmt1 activity of the phosphoenolpyruvate-dependent phosphotransferase system all map in gene mtlA. This gene has previously been shown (Solomon and Lin, 1972) to be part of an operon, mtl, located at 71 min on the E. coli linkage map containing, in addition to mtlA, the cis-dominant regulatory gene mtlC and mtlD, the structural gene for the enzyme D-mannitol-1-phosphate dehydrogenase. The gene order in this operon, induced by D-mannitol, is mtlC A D. (ii) Mutations in gene gutA affecting a second enzyme II-complexgut of the phosphotransferase system map at 51 min, clustered in operon gutC A D together with the cis-dominant regulatory gene gutC and the structural gene gutD for the enzyme D-glucitol-6-phosphate dehydrogenase. The gut operon, previously called sbl or srl, is induced by D-glucitol. (iii) Mutations affecting the transport and catabolism of galactitol are clustered in a third operon, gatC A D, located at 40.5 min. This operon again contains a cis-dominant regulatory gene, gatC, the structural gene gatD for galactitol-1-phosphate dehydrogenase, and gene gatA coding for a thrid hexitol-specific enzyme II-complexgat. Other genes coding for two additional enzymes involved in galactitol catabolism apparently are not linked to gatC A D. (iv) A fourth class of mutants pleiotropically negative for hexitol growth and transport maps in the pts operon. Triple-negative mutants (mtlA gutA gatA) do not have further transport or phosphorylating activity for any of the three hexitols."}
{"id": "PMID:1100604", "title": "\"Killer\" character does not influence the transmission of mitochondrial genes in Saccharomyces cerevisiae.", "content": "Three cytoplasmic genetic elements have been shown to be separate from mitochondrial deoxyribonucleic acid (DNA), [rho], in Saccharomyces cerevisiae: the killer character [k], omicron-DNA, and psi [psi]. Griffiths has suggested genetic interactions between [VENR] and [TETR] mutants possibly located on omicron-DNA and mitochondrial genetic markers, but possible interactions between the best characterized of the three, the killer character, and mitochondrial DNA have not been investigated. To test this we isolated cycloheximide-induced nonkiller segregants (NKS) of killer cells with suitable genetic markers and mated them in [k] x [k], [k] x [k], and (NKS) x (NKS) combinations. No differences in quantitative mitochondrial marker transmission between these groups were found in crosses illustrating the mitochondrial phenomena of bias, polarity, and suppressiveness. Our studies show that no intercellular interactions between [k] and (NKS) cells influence mitochondrial transmission genetics. Intracellular interactions between the smaller double-stranded ribonucleic acid of [k] and mitochondrial DNA also were not detected.", "contents": "\"Killer\" character does not influence the transmission of mitochondrial genes in Saccharomyces cerevisiae. Three cytoplasmic genetic elements have been shown to be separate from mitochondrial deoxyribonucleic acid (DNA), [rho], in Saccharomyces cerevisiae: the killer character [k], omicron-DNA, and psi [psi]. Griffiths has suggested genetic interactions between [VENR] and [TETR] mutants possibly located on omicron-DNA and mitochondrial genetic markers, but possible interactions between the best characterized of the three, the killer character, and mitochondrial DNA have not been investigated. To test this we isolated cycloheximide-induced nonkiller segregants (NKS) of killer cells with suitable genetic markers and mated them in [k] x [k], [k] x [k], and (NKS) x (NKS) combinations. No differences in quantitative mitochondrial marker transmission between these groups were found in crosses illustrating the mitochondrial phenomena of bias, polarity, and suppressiveness. Our studies show that no intercellular interactions between [k] and (NKS) cells influence mitochondrial transmission genetics. Intracellular interactions between the smaller double-stranded ribonucleic acid of [k] and mitochondrial DNA also were not detected."}
{"id": "PMID:1100605", "title": "Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli.", "content": "the mutation that causes ribonuclease III (RNase III) deficiency in strain AB301-105 of Kindler et al. (1973) has been mapped by use of F' merodiploids, Hfr matings, and P1 transduction. This mutation, rnc-105, lies close to nadB, near 49 min on the genetic map of Escherichia coli. The rnc-105 mutation has been transferred from its original genetic background by transduction and conjugation, and these new strains have the same defects in ribonucleic acid processing reported previously for AB301-105. Strains that carry rnc-105 grow more slowly than parental rnc+ strains, but the difference in growth rate seems to depend on the genetic background of each strain. Bacteriophage T7 grows about equally well in RNase III+ and III- female strains of E. coli, even though the specific cuts that RNase III makes in T7 ribonucleic acid are not made in the RNase III- strains. A low-phosphate defined medium in which most E. coli strains seem to grow well was developed. This medium is equally useful for labeling ribonucleic acids with 32PO4 and as a selective medium for genetic manipulations. It was used to determine the growth requirements of strain AB301-105, which are biotin and succinate in addition to the methionine and histidine requirements of the parental strain. The biotin mutation lies near the position expected from known mutations of E. coli, but the succinate mutation apparently does not. The possibility that the succinate requirement could be due to the RNase III deficiency is discussed. A uraP mutation was isolated for use in transferring rnc-105 between strains by conjugation. It lies near 47 min, somewhat removed from the commonly accepted position for uraP.", "contents": "Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli. the mutation that causes ribonuclease III (RNase III) deficiency in strain AB301-105 of Kindler et al. (1973) has been mapped by use of F' merodiploids, Hfr matings, and P1 transduction. This mutation, rnc-105, lies close to nadB, near 49 min on the genetic map of Escherichia coli. The rnc-105 mutation has been transferred from its original genetic background by transduction and conjugation, and these new strains have the same defects in ribonucleic acid processing reported previously for AB301-105. Strains that carry rnc-105 grow more slowly than parental rnc+ strains, but the difference in growth rate seems to depend on the genetic background of each strain. Bacteriophage T7 grows about equally well in RNase III+ and III- female strains of E. coli, even though the specific cuts that RNase III makes in T7 ribonucleic acid are not made in the RNase III- strains. A low-phosphate defined medium in which most E. coli strains seem to grow well was developed. This medium is equally useful for labeling ribonucleic acids with 32PO4 and as a selective medium for genetic manipulations. It was used to determine the growth requirements of strain AB301-105, which are biotin and succinate in addition to the methionine and histidine requirements of the parental strain. The biotin mutation lies near the position expected from known mutations of E. coli, but the succinate mutation apparently does not. The possibility that the succinate requirement could be due to the RNase III deficiency is discussed. A uraP mutation was isolated for use in transferring rnc-105 between strains by conjugation. It lies near 47 min, somewhat removed from the commonly accepted position for uraP."}
{"id": "PMID:1100606", "title": "Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid.", "content": "Localization of a mutation affecting ribonuclease III activity (an enzyme specific for double-stranded ribonucleic acid) in Escherichia coli was attempted. By a series of matings and transduction experiments, the mutation rnc-105 was mapped near the nadB gene. In strains carrying this mutation, another mutation (ranA2074) was also found. Based on available data, their order on the E. coli chromosome appears to be tyrA, ranA, nadB, rnc, purI. Strains carrying either the ranA2074 or the rnc-105 mutation fail to grow at 45 C in enriched medium, whereas strains carrying only the rnc-105 mutation are defective in ribonuclease III activity. Strains carrying either of these mutations grow more slowly than corresponding wild-type strains in all media tested at all temperatures; the rnc-105 mutation reduces the growth rate more than the ranA2074 mutation. T4 and T7 bacteriophages form plaques with a lower efficiency on strains carrying the rnc-105 mutation than on other strains. Thus we suggest that ribonuclease III is beneficial for normal growth of E. Coli and that at higher temperatures it becomes indispensable.", "contents": "Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid. Localization of a mutation affecting ribonuclease III activity (an enzyme specific for double-stranded ribonucleic acid) in Escherichia coli was attempted. By a series of matings and transduction experiments, the mutation rnc-105 was mapped near the nadB gene. In strains carrying this mutation, another mutation (ranA2074) was also found. Based on available data, their order on the E. coli chromosome appears to be tyrA, ranA, nadB, rnc, purI. Strains carrying either the ranA2074 or the rnc-105 mutation fail to grow at 45 C in enriched medium, whereas strains carrying only the rnc-105 mutation are defective in ribonuclease III activity. Strains carrying either of these mutations grow more slowly than corresponding wild-type strains in all media tested at all temperatures; the rnc-105 mutation reduces the growth rate more than the ranA2074 mutation. T4 and T7 bacteriophages form plaques with a lower efficiency on strains carrying the rnc-105 mutation than on other strains. Thus we suggest that ribonuclease III is beneficial for normal growth of E. Coli and that at higher temperatures it becomes indispensable."}
{"id": "PMID:1100607", "title": "Transfer ribonucleic acid methylase deficiency found in UGA supressor strains.", "content": "Extracts of recessive UGA suppressor strains, designated supK, are deficient in transfer ribonucleic acid (tRNA)-methylating activity when compared to wild-type extracts. Moreover, the tRNA from suppressor strains is methyl deficient when compared to wild-type tRNA. This deficiency is due to the lack of a single tRNA methylase activity in suppressor strains. UGA suppressor activity may be caused by the miscoding of one or more methyl-deficient tRNA's in supK strains.", "contents": "Transfer ribonucleic acid methylase deficiency found in UGA supressor strains. Extracts of recessive UGA suppressor strains, designated supK, are deficient in transfer ribonucleic acid (tRNA)-methylating activity when compared to wild-type extracts. Moreover, the tRNA from suppressor strains is methyl deficient when compared to wild-type tRNA. This deficiency is due to the lack of a single tRNA methylase activity in suppressor strains. UGA suppressor activity may be caused by the miscoding of one or more methyl-deficient tRNA's in supK strains."}
{"id": "PMID:1100608", "title": "Nature and properties of hexitol transport systems in Escherichia coli.", "content": "In Escherichia coli K-12 the naturally occurring hexitols D-mannitol, D-glucitol, and galactitol are taken up and phosphorylated via three distinct transport systems by a mechanism called either group translocation or vectorial phosphorylation. For every system, a membrane-bound enzyme II-complex of the phosphoenolpyruvate-dependent phosphotransferase system has been found, each requiring phosphoenolpyruvate, enzyme I, and HPr or alternatively P-HPr as the phosphate donor. Cells with a constitutive synthesis of all hexitol transport systems but with low P-HPr levels have very low transport and phosphorylating activities in vivo, although 40 to 90% of the enzyme II-complex activities are detected in cell extracts of such mutants. No indications for additional hexitol transport systems, especially for systems able to transport and accumulate free hexitols as in Klebsiella aerogenes, have been found. Substrate Km, and Vmax of the three transport systems for several hexitols and hexitol analogues have been determined by growth rates, transport activities, and in vitro phosphorylating activities. Each system was found to take up several hexitols, but only one hexitol serves as the inducer. This inducer invariably is the substrate with the highest affinity. Since bacterial transport systems, as a general rule, seem to have a relatively broad substrate specificity, in contrast to a more restricted inducer specificity, we propose to name the system inducible by D-mannitol and coded by the gene mtlA the D-mannitol transport system, the system inducible by D-glucitol and coded by gutA the D-glucitol transport system, and the system inducible by galactitol and coded by gatA the galactitol transport system.", "contents": "Nature and properties of hexitol transport systems in Escherichia coli. In Escherichia coli K-12 the naturally occurring hexitols D-mannitol, D-glucitol, and galactitol are taken up and phosphorylated via three distinct transport systems by a mechanism called either group translocation or vectorial phosphorylation. For every system, a membrane-bound enzyme II-complex of the phosphoenolpyruvate-dependent phosphotransferase system has been found, each requiring phosphoenolpyruvate, enzyme I, and HPr or alternatively P-HPr as the phosphate donor. Cells with a constitutive synthesis of all hexitol transport systems but with low P-HPr levels have very low transport and phosphorylating activities in vivo, although 40 to 90% of the enzyme II-complex activities are detected in cell extracts of such mutants. No indications for additional hexitol transport systems, especially for systems able to transport and accumulate free hexitols as in Klebsiella aerogenes, have been found. Substrate Km, and Vmax of the three transport systems for several hexitols and hexitol analogues have been determined by growth rates, transport activities, and in vitro phosphorylating activities. Each system was found to take up several hexitols, but only one hexitol serves as the inducer. This inducer invariably is the substrate with the highest affinity. Since bacterial transport systems, as a general rule, seem to have a relatively broad substrate specificity, in contrast to a more restricted inducer specificity, we propose to name the system inducible by D-mannitol and coded by the gene mtlA the D-mannitol transport system, the system inducible by D-glucitol and coded by gutA the D-glucitol transport system, and the system inducible by galactitol and coded by gatA the galactitol transport system."}
{"id": "PMID:1100609", "title": "Effects on specific antibodies on the catalytic activity of L-asparaginase from Serratia marcescens and Escherichia coli.", "content": "Rabbit antisera against highly purified L-asparaginase from Serratia marcescens and from Escherichia coli showed up to 60% inhibition of the catalytic amidohydrolysis of L-asparagine when combined with the homologous enzyme. This inhibition was diminished somewhat against the heterologous enzyme. Kinetic studies in the presence of these antisera showed an increased Kmapp for both homologous and heterologous enzymes using L-asparagine as substrate. In contrast, kinetic studies employing the poor substrate, L-glutamine, showed activation attributable to specific antibodies. This was seen in lower Kmapp values and up to twofold increases in the Vmax over the normal rabbit serum controls. The high degree of cross-inhibition (approximately 80%) and the low degree of cross-reactivity in the quantitative precipitin test (approximately 34%) suggest that these two enzymes possess structural similarities located mainly in the regions of the catalytic sites.", "contents": "Effects on specific antibodies on the catalytic activity of L-asparaginase from Serratia marcescens and Escherichia coli. Rabbit antisera against highly purified L-asparaginase from Serratia marcescens and from Escherichia coli showed up to 60% inhibition of the catalytic amidohydrolysis of L-asparagine when combined with the homologous enzyme. This inhibition was diminished somewhat against the heterologous enzyme. Kinetic studies in the presence of these antisera showed an increased Kmapp for both homologous and heterologous enzymes using L-asparagine as substrate. In contrast, kinetic studies employing the poor substrate, L-glutamine, showed activation attributable to specific antibodies. This was seen in lower Kmapp values and up to twofold increases in the Vmax over the normal rabbit serum controls. The high degree of cross-inhibition (approximately 80%) and the low degree of cross-reactivity in the quantitative precipitin test (approximately 34%) suggest that these two enzymes possess structural similarities located mainly in the regions of the catalytic sites."}
{"id": "PMID:1100610", "title": "Lag in adaptation to lactose as a probe to the timing of permease incorporation into the cell membrane.", "content": "If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an inducible culture to low lactose provides such a situation since the cells cannot grow unless galactoside permease can function to concentrate the lactose internally. From such experiments, it was concluded that the Y gene product of the lac operon is synthesized, incorporated, and can start functioning in active transport, at any time throughout the bulk of the cell cycle. Not only were the lags before growth re-ensued much shorter than would be expected if the membrane transport capability could only be developed in  a small portion of the cycle, but brief pulses of a gratuitous inducer shortened the lags much further. Three types of Escherichia coli ML 30 culture were studied: cells that had exhausted the limiting glucose; cells taken directly from glucose-limited chemostats; and a washed suspension of highly catabolite repressed cells from cultures grown in high levels of glucose and gluconate. The growth studies reported here were performed on-line with a minicomputer. They represent at least an order of magnitude increase in accuracy in estimating growth parameters over previous instrumentation.", "contents": "Lag in adaptation to lactose as a probe to the timing of permease incorporation into the cell membrane. If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an inducible culture to low lactose provides such a situation since the cells cannot grow unless galactoside permease can function to concentrate the lactose internally. From such experiments, it was concluded that the Y gene product of the lac operon is synthesized, incorporated, and can start functioning in active transport, at any time throughout the bulk of the cell cycle. Not only were the lags before growth re-ensued much shorter than would be expected if the membrane transport capability could only be developed in  a small portion of the cycle, but brief pulses of a gratuitous inducer shortened the lags much further. Three types of Escherichia coli ML 30 culture were studied: cells that had exhausted the limiting glucose; cells taken directly from glucose-limited chemostats; and a washed suspension of highly catabolite repressed cells from cultures grown in high levels of glucose and gluconate. The growth studies reported here were performed on-line with a minicomputer. They represent at least an order of magnitude increase in accuracy in estimating growth parameters over previous instrumentation."}
{"id": "PMID:1100611", "title": "Isolation of plasmid deoxyribonucleic acid from two strains of Bacteroides.", "content": "Two clinical isolates of Bacteroides contained covalently closed circular deoxyribonucleic acid (DNA) as shown by sedimentation in an alkaline sucrose gradient, CsCl ethidium bromide equilibrium centrifugation, and electron microscopy. Bacteriodes fragilis N1175 contained a homogeneous species of plasmid DNA with a molecular weight of 25 x 10(6). Bacteroides ochraceus 2228 contained two distinct, covalently closed circular DNA elements. The larger cosedimented with the covalently closed circular DNA form of the R plasmid, R100, corresponding to a molecular weight of 70 x 10(6); the smaller sedimented as a 58S molecule with a calculated molecular weight of 25 x 10(6). The roles of these plasmids are unknown. Neither strain transferred antibiotic resistance to plasmid-negative Bacteroides or Escherichia coli, and neither produced bacteriocins active against other Bacteroides or sensitive indicator strains of E. coli.", "contents": "Isolation of plasmid deoxyribonucleic acid from two strains of Bacteroides. Two clinical isolates of Bacteroides contained covalently closed circular deoxyribonucleic acid (DNA) as shown by sedimentation in an alkaline sucrose gradient, CsCl ethidium bromide equilibrium centrifugation, and electron microscopy. Bacteriodes fragilis N1175 contained a homogeneous species of plasmid DNA with a molecular weight of 25 x 10(6). Bacteroides ochraceus 2228 contained two distinct, covalently closed circular DNA elements. The larger cosedimented with the covalently closed circular DNA form of the R plasmid, R100, corresponding to a molecular weight of 70 x 10(6); the smaller sedimented as a 58S molecule with a calculated molecular weight of 25 x 10(6). The roles of these plasmids are unknown. Neither strain transferred antibiotic resistance to plasmid-negative Bacteroides or Escherichia coli, and neither produced bacteriocins active against other Bacteroides or sensitive indicator strains of E. coli."}
{"id": "PMID:1100612", "title": "Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae.", "content": "The interdependence of spindle plaque with other aspects of cell division and conjugation in Saccharomyces cerevisiae has been investigated. Three forms of the spindle plaque appear sequentially before the formation of the complete, intranuclear spindle. The single plaque is present initially in the mitotic cycle; it becomes transformed into a satellite-bearing single plaque during the latter part of G1. Subsequently, plaque duplication yields the double plaque characteristic of the early phase of budding, which coincides with the period of chromosome replication (S). The eventual separation of these plaques to form a complete spindle, with a single plaque at each pole, is nearly coincident with the completion of S. The form of the plaque differs in two independent cases of G1 arrest: the single plaque is found in a cell in stationary arrest of growth, whereas a cell arrested by mating factors in preparation for conjugation contains a satellite-bearing single plaque. The latter form is retained during zygote formation, where it serves as the initial site of fusion of each prezygotic nuceus with the other. This fusion results in the formation of  a single zygotic nucleus with a satellite-bearing single plaque, which is subsequently transformed into a double plaque as the zygote buds. The double plaque is situated adjacent to the site of bud emergence in both vegetative cells and zygotes.", "contents": "Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae. The interdependence of spindle plaque with other aspects of cell division and conjugation in Saccharomyces cerevisiae has been investigated. Three forms of the spindle plaque appear sequentially before the formation of the complete, intranuclear spindle. The single plaque is present initially in the mitotic cycle; it becomes transformed into a satellite-bearing single plaque during the latter part of G1. Subsequently, plaque duplication yields the double plaque characteristic of the early phase of budding, which coincides with the period of chromosome replication (S). The eventual separation of these plaques to form a complete spindle, with a single plaque at each pole, is nearly coincident with the completion of S. The form of the plaque differs in two independent cases of G1 arrest: the single plaque is found in a cell in stationary arrest of growth, whereas a cell arrested by mating factors in preparation for conjugation contains a satellite-bearing single plaque. The latter form is retained during zygote formation, where it serves as the initial site of fusion of each prezygotic nuceus with the other. This fusion results in the formation of  a single zygotic nucleus with a satellite-bearing single plaque, which is subsequently transformed into a double plaque as the zygote buds. The double plaque is situated adjacent to the site of bud emergence in both vegetative cells and zygotes."}
{"id": "PMID:1100613", "title": "Sizing of bdellovibrio during growth.", "content": "A technique for the counting and relative sizing of host-independent bdellovibrio during growth, with the aid of a modified Coulter counter, is described.", "contents": "Sizing of bdellovibrio during growth. A technique for the counting and relative sizing of host-independent bdellovibrio during growth, with the aid of a modified Coulter counter, is described."}
{"id": "PMID:1100614", "title": "Recipient ability of bacteriophage-resistant mutants of Escherichia coli K-12.", "content": "The ability of a wide range of bacteriophage-resistant mutants to act as recipients in conjugation with F'lac pro and R100-1 donors has been studied. A number of mutant types defective in recipient ability with F'lac pro, as well as mutants which were hyperrecptive with R100-1, have been detected.", "contents": "Recipient ability of bacteriophage-resistant mutants of Escherichia coli K-12. The ability of a wide range of bacteriophage-resistant mutants to act as recipients in conjugation with F'lac pro and R100-1 donors has been studied. A number of mutant types defective in recipient ability with F'lac pro, as well as mutants which were hyperrecptive with R100-1, have been detected."}
{"id": "PMID:1100615", "title": "Bacteriophage Mu-1-induced permeability mutants in Escherichia coli K-12.", "content": "Apparent permeability mutations were produced in Escherichia coli K-12 by bacteriophage mu-1 mutagenesis. They are pleiotropic mutations showing sensitivity to a number of detergents and unrelated antibiotics, and presumably they affect cell wall or membrane biosynthesis. One of the mutations was genetically mapped at a site in or near the acrA and mtc loci at approximately 10.5 min on the Taylor and Trotter map (1972).", "contents": "Bacteriophage Mu-1-induced permeability mutants in Escherichia coli K-12. Apparent permeability mutations were produced in Escherichia coli K-12 by bacteriophage mu-1 mutagenesis. They are pleiotropic mutations showing sensitivity to a number of detergents and unrelated antibiotics, and presumably they affect cell wall or membrane biosynthesis. One of the mutations was genetically mapped at a site in or near the acrA and mtc loci at approximately 10.5 min on the Taylor and Trotter map (1972)."}
{"id": "PMID:1100616", "title": "Characterization of V-prime factors in Escherichia coli K-12.", "content": "An episome derived from an Hfr(v) (Hfr isolated from a V colicinogenic parent) strain of Escherichia coli K-12 was isolated and characterized. The direction of gene transfer was inverted from that in the original parental strain.", "contents": "Characterization of V-prime factors in Escherichia coli K-12. An episome derived from an Hfr(v) (Hfr isolated from a V colicinogenic parent) strain of Escherichia coli K-12 was isolated and characterized. The direction of gene transfer was inverted from that in the original parental strain."}
{"id": "PMID:1100617", "title": "Transductional mapping of gene trmA responsible for the production of 5-methyluridine in transfer ribonucleic acid of Escherichia coli.", "content": "The gene trmA, responsible for the production of 5-methyluridine (ribothymidine) in transfer ribonucleic acid, has been located at 79 min on the chromosomal map of Escherichia coli K-12. In five-factor crosses the gene order was shown to be argH-trmA-rif-thiA-metA. The co-transduction frequency between argH and trmA was 65%. Furthermore, the trmA5 mutation was shown to be recessive, in agreement with the notion that the trmA gene is the structural gene for the transfer tibonucleic acid (5-methyluridine) methyltransferase.", "contents": "Transductional mapping of gene trmA responsible for the production of 5-methyluridine in transfer ribonucleic acid of Escherichia coli. The gene trmA, responsible for the production of 5-methyluridine (ribothymidine) in transfer ribonucleic acid, has been located at 79 min on the chromosomal map of Escherichia coli K-12. In five-factor crosses the gene order was shown to be argH-trmA-rif-thiA-metA. The co-transduction frequency between argH and trmA was 65%. Furthermore, the trmA5 mutation was shown to be recessive, in agreement with the notion that the trmA gene is the structural gene for the transfer tibonucleic acid (5-methyluridine) methyltransferase."}
{"id": "PMID:1100618", "title": "Physiological and biochemical studies on the function of 5-methyluridine in the transfer ribonucleic acid of Escherichia coli.", "content": "Matched pairs of transductant strains differing by the presence of absence of 5-methyluridine (ribothymidine) (m5U) in their transfer ribonucleic acid (tRNA) were used to study the function of this modified nucleoside in Escherichia coli. Ordinary measurements of growth rate in different media revealed no effect of the loss of m5U in tRNA. A gene located close to trmA (the structural cistron for the methyltransferase that produces m5U in tRNA), however, was found to reduce the growth rates significantly, depending on the medium and the temperature of cultivation. Measurement of codon recognition, macromolecular composition, tRNA binding to the ribosome, and the rate of protein chain elongation in vivo indicated no disadvantage caused by the lack of m5U. The regulation of ilv and his operons seemed also to be unaffected by the absence of m5U in the tRNA. In a mixed population experiment, however, cells possessing m5U in their tRNA seemed to have a distinct advantage over cells lacking this modified nucleoside. This experiment provides the first indication of the overall value of m5U in tRNA.", "contents": "Physiological and biochemical studies on the function of 5-methyluridine in the transfer ribonucleic acid of Escherichia coli. Matched pairs of transductant strains differing by the presence of absence of 5-methyluridine (ribothymidine) (m5U) in their transfer ribonucleic acid (tRNA) were used to study the function of this modified nucleoside in Escherichia coli. Ordinary measurements of growth rate in different media revealed no effect of the loss of m5U in tRNA. A gene located close to trmA (the structural cistron for the methyltransferase that produces m5U in tRNA), however, was found to reduce the growth rates significantly, depending on the medium and the temperature of cultivation. Measurement of codon recognition, macromolecular composition, tRNA binding to the ribosome, and the rate of protein chain elongation in vivo indicated no disadvantage caused by the lack of m5U. The regulation of ilv and his operons seemed also to be unaffected by the absence of m5U in the tRNA. In a mixed population experiment, however, cells possessing m5U in their tRNA seemed to have a distinct advantage over cells lacking this modified nucleoside. This experiment provides the first indication of the overall value of m5U in tRNA."}
{"id": "PMID:1100620", "title": "Uridine diphosphate galactose-4-epimerase. Uridine monophosphate-dependent reduction by alpha- and beta-D-glucose.", "content": "Rates of UMP-dependent reduction of the DPN+ associated with Escherichia coli UDP-galactose-4-epimerase at 27 degrees and 0.2 M ionic strength in 0.1 M Tris-HCl buffer, pH 8.5, are reported. The reaction exhibits excellent pseudo-first order behavior when D-glucose is at anomeric equilibrium. The effects of [UMP] and [glucose] on the observed first order rate constants are consistent with the following equation. The symbols phi are empirical parameters. (See article). The data indicate that the pathway involves random equilibrium binding of UMP and glucose followed by rate-limiting decomposition of the ternary complex to epimerase-DNPH. The binding parameters indicate that the principal activating effect of UMP is not simply to increase the affinity of the enzyme for glucose. UMP appears to increase the reactivity or availability of enzyme-bound DPN+. The kinetic isotope effect for the reaction of D-]1-2H]glucose (kH/kD) is 4.2, which confirms that C-1 is oxidized and that hydride transfer is rate limiting. Both of the purified anomers, alpha- and beta-D-glucose, reduce the enzyme-bound DPN+. As indicated by the deviations from pseudo-first order kinetics because of concurrent mutarotation, the beta anomer is the more reactive, reacting about 4 to 5 times faster than the alpha anomer at concentrations well below saturation. Is is suggested that the lack of stereo-specificity in this reaction may be attributed to the two anomers being productively bound with their opposite faces projecting toward C-4 of bound DPN+. Nonstereospecific oxidation of alpha- and beta-D-glucose may be a model for the mechanism of UDP-hexose epimerization, which also involves nonstereospecific hydride transfer.", "contents": "Uridine diphosphate galactose-4-epimerase. Uridine monophosphate-dependent reduction by alpha- and beta-D-glucose. Rates of UMP-dependent reduction of the DPN+ associated with Escherichia coli UDP-galactose-4-epimerase at 27 degrees and 0.2 M ionic strength in 0.1 M Tris-HCl buffer, pH 8.5, are reported. The reaction exhibits excellent pseudo-first order behavior when D-glucose is at anomeric equilibrium. The effects of [UMP] and [glucose] on the observed first order rate constants are consistent with the following equation. The symbols phi are empirical parameters. (See article). The data indicate that the pathway involves random equilibrium binding of UMP and glucose followed by rate-limiting decomposition of the ternary complex to epimerase-DNPH. The binding parameters indicate that the principal activating effect of UMP is not simply to increase the affinity of the enzyme for glucose. UMP appears to increase the reactivity or availability of enzyme-bound DPN+. The kinetic isotope effect for the reaction of D-]1-2H]glucose (kH/kD) is 4.2, which confirms that C-1 is oxidized and that hydride transfer is rate limiting. Both of the purified anomers, alpha- and beta-D-glucose, reduce the enzyme-bound DPN+. As indicated by the deviations from pseudo-first order kinetics because of concurrent mutarotation, the beta anomer is the more reactive, reacting about 4 to 5 times faster than the alpha anomer at concentrations well below saturation. Is is suggested that the lack of stereo-specificity in this reaction may be attributed to the two anomers being productively bound with their opposite faces projecting toward C-4 of bound DPN+. Nonstereospecific oxidation of alpha- and beta-D-glucose may be a model for the mechanism of UDP-hexose epimerization, which also involves nonstereospecific hydride transfer."}
{"id": "PMID:1100621", "title": "Reactivation of the lipid-depleted pyruvate oxidase system from Escherichia coli with cell envelope neutral lipids.", "content": "The pyruvate oxidase system of Escherichia coli is composed of a soluble flavoprotein, pyruvate oxidase (EC 1.2.2.2, pyruvate:ferricytochrome b1 oxidoreductase), and an electron transport system associated with the cell envelope-membrane fraction. The membrane particles contain 15% lipid by weight. Fractionation of the lipids revealed that abut one-third are neutral lipids and two-thirds are phospholipids. The relative ratio of ubiquinone to menaquinone within the neutral lipid fraction is 15:1 on a molar basis. Removal of the lipids from the membrane particles by extraction with aqueous acetone or hydrolysis of the phospholipids by treatment with Bacillus cereus phospholipase C results in a complete loss of electron transport activity. Analysis of the particles extracted with aqueous acetone revealed that practically all the neutral lipids and 65% of the phospholipids are removed by this treatment. Phospholipase treatment results in a loss of 75% of the membrane phospholipid phosphorus; however, the diglycerides and the neutral lipids produced by phospholipase hydrolysis remain associated with the particles. Addition of neutral lipid and a detergent, hepta-DL-alanyl dodecylamide to the acetone-extracted material results in a restoration of 37% of the original particle activity. Addition of neutral lipid and hepta-DL-alanyl dodecylamide to phospholipase-treated particles completely restores the original electron transport activity. Furthermore, addition of ubiquinone from either yeast (UQ6) or E. coli (UQ8) will restore pyruvate oxidase activity when the quinones are supplemented with photoinactivated neutral lipid. No restoration of activity to phospholipase-treated particles is noted upon the addition of either menaquinone 6 or menaquinone 8 to the reconstitution system. In fact, these compounds appear to suppress restoration of activity when they are added to reaction mixtures containing neutral lipid and phospholipase-treated particles.", "contents": "Reactivation of the lipid-depleted pyruvate oxidase system from Escherichia coli with cell envelope neutral lipids. The pyruvate oxidase system of Escherichia coli is composed of a soluble flavoprotein, pyruvate oxidase (EC 1.2.2.2, pyruvate:ferricytochrome b1 oxidoreductase), and an electron transport system associated with the cell envelope-membrane fraction. The membrane particles contain 15% lipid by weight. Fractionation of the lipids revealed that abut one-third are neutral lipids and two-thirds are phospholipids. The relative ratio of ubiquinone to menaquinone within the neutral lipid fraction is 15:1 on a molar basis. Removal of the lipids from the membrane particles by extraction with aqueous acetone or hydrolysis of the phospholipids by treatment with Bacillus cereus phospholipase C results in a complete loss of electron transport activity. Analysis of the particles extracted with aqueous acetone revealed that practically all the neutral lipids and 65% of the phospholipids are removed by this treatment. Phospholipase treatment results in a loss of 75% of the membrane phospholipid phosphorus; however, the diglycerides and the neutral lipids produced by phospholipase hydrolysis remain associated with the particles. Addition of neutral lipid and a detergent, hepta-DL-alanyl dodecylamide to the acetone-extracted material results in a restoration of 37% of the original particle activity. Addition of neutral lipid and hepta-DL-alanyl dodecylamide to phospholipase-treated particles completely restores the original electron transport activity. Furthermore, addition of ubiquinone from either yeast (UQ6) or E. coli (UQ8) will restore pyruvate oxidase activity when the quinones are supplemented with photoinactivated neutral lipid. No restoration of activity to phospholipase-treated particles is noted upon the addition of either menaquinone 6 or menaquinone 8 to the reconstitution system. In fact, these compounds appear to suppress restoration of activity when they are added to reaction mixtures containing neutral lipid and phospholipase-treated particles."}
{"id": "PMID:1100622", "title": "Evidence for the coordinate control of glycogen synthesis, glucose utilization, and glycolysis in Escherichia coli. I. Quantitative covariance of the rate of glucose utilization and the cellular level of fructose 1,6-diphosphate during exponential growth and nutrient limitation.", "content": "In cultures of Escherichia coli W4597(K) and G34 under various nutritional conditions the rates of glucose utilization and cellular levels of fructose-1,6-P2 are quantitatively related by the Hill equation where the value of the Hill coefficient is approximately equal to 2. This is the first evidence that fructose-P2, or any metabolite which covaries with fructose-P2, modulates glucose utilization in E. coli. In light of previous observations from our laboratory this new observation and those in the succeeding report provide the first evidence that in E. coli glycolysis, glycogen synthesis and glucose utilization are coordinately regulated, thus providing for the coupling of ATP utilization and production under various metabolic circumstances. Alterations in the level of ATP apparently affect the velocity of phosphofructokinase, the rate-limiting enzyme in glycolysis, altering the cellular levels of glucose-6-P or fructose-P2. Changes in the levels of these hexose phosphates are quantitatively related to alterations in the rates of glucose utilization and glycogen synthesis in the intact E. coli cell.", "contents": "Evidence for the coordinate control of glycogen synthesis, glucose utilization, and glycolysis in Escherichia coli. I. Quantitative covariance of the rate of glucose utilization and the cellular level of fructose 1,6-diphosphate during exponential growth and nutrient limitation. In cultures of Escherichia coli W4597(K) and G34 under various nutritional conditions the rates of glucose utilization and cellular levels of fructose-1,6-P2 are quantitatively related by the Hill equation where the value of the Hill coefficient is approximately equal to 2. This is the first evidence that fructose-P2, or any metabolite which covaries with fructose-P2, modulates glucose utilization in E. coli. In light of previous observations from our laboratory this new observation and those in the succeeding report provide the first evidence that in E. coli glycolysis, glycogen synthesis and glucose utilization are coordinately regulated, thus providing for the coupling of ATP utilization and production under various metabolic circumstances. Alterations in the level of ATP apparently affect the velocity of phosphofructokinase, the rate-limiting enzyme in glycolysis, altering the cellular levels of glucose-6-P or fructose-P2. Changes in the levels of these hexose phosphates are quantitatively related to alterations in the rates of glucose utilization and glycogen synthesis in the intact E. coli cell."}
{"id": "PMID:1100623", "title": "Evidence for the coordinate control of glycogen synthesis, glucose utilization, and glycolysis in Escherichia coli. II. Quantitative correlation of the inhibition of glycogen synthesis and the stimulation of glucose utilization by 2,4-dinitrophenol with the effects on the cellular levels of glucose 6-phosphate, fructose, 1,6-diphosphate, and total adenylates.", "content": "In cultures of Escherichia coli W4597(K) and G34 under various nutritional conditions the rates of glucose utilization and cellular levels of fructose-1,6-P2 are quantitatively related by the Hill equation where the value of the Hill coefficient is approximately equal to 2. This is the first evidence that fructose-P2, or any metabolite which covaries with fructose-P2, modulates glucose utilization in E. coli. In light of previous observations from our laboratory this new observation and those in the succeeding report provide the first evidence that in E. coli glycolsis, glycogen synthesis and glucose utilization are coordinately regulated, thus providing for the coupling of ATP utilization and production under various metabolic circumstances. Alterations in the level of ATP apparently affect the velocity of phosphofructokinase, the rate-limiting enzyme in glycolsis, altering the cellular levels of glucose-6-P or fructose-P2. Changes in the levels of these hexose phosphates are quantitatively related to alterations in the rates of glucose utilization and glycogen synthesis in the intact E. coli cell.", "contents": "Evidence for the coordinate control of glycogen synthesis, glucose utilization, and glycolysis in Escherichia coli. II. Quantitative correlation of the inhibition of glycogen synthesis and the stimulation of glucose utilization by 2,4-dinitrophenol with the effects on the cellular levels of glucose 6-phosphate, fructose, 1,6-diphosphate, and total adenylates. In cultures of Escherichia coli W4597(K) and G34 under various nutritional conditions the rates of glucose utilization and cellular levels of fructose-1,6-P2 are quantitatively related by the Hill equation where the value of the Hill coefficient is approximately equal to 2. This is the first evidence that fructose-P2, or any metabolite which covaries with fructose-P2, modulates glucose utilization in E. coli. In light of previous observations from our laboratory this new observation and those in the succeeding report provide the first evidence that in E. coli glycolsis, glycogen synthesis and glucose utilization are coordinately regulated, thus providing for the coupling of ATP utilization and production under various metabolic circumstances. Alterations in the level of ATP apparently affect the velocity of phosphofructokinase, the rate-limiting enzyme in glycolsis, altering the cellular levels of glucose-6-P or fructose-P2. Changes in the levels of these hexose phosphates are quantitatively related to alterations in the rates of glucose utilization and glycogen synthesis in the intact E. coli cell."}
{"id": "PMID:1100624", "title": "Studies on the mechanism of inhibition of Salmonella typhimurium by 1,2,4-triazole.", "content": "The inhibition of Salmonella typhimurium by 1,2,4-triazole appears to be mediated through an effect on L-cysteine biosynthesis. O-Acetylserine sulfhydrylase A, the final enzyme in the L-cysteine biosynthetic pathway, was found to catalyze a reaction (triazolylase) between O-acetyl-L-serine and 1,2,4-triazole, giving 1,2,4-triazole-1-alanine as a product. In wild type S. typhimurium grown on 4 mM 1,2,4-triazole, 97% of the total O-acetyl-L-serine synthesized in vivo is incorporated into 1,2,4-triazole-1-alanine. 1,2,4-triazole also significantly lowers the levels of several of the enzymes necessary for sulfate reduction. This effect is presumably due to the ability of the inhibitor to lower intracellular concentrations of O-acetyl-L-serine, an inducer of these enzymes. Inhibition of growth is probably caused by L-cysteine starvation, arising from the decreased availability of the L-cysteine precursors, sulfide and O-acetyl-L-serine. Two 1,2,4-triazole-resistant strains bearing mutations in cysK, the structural gene for O-acetylserine sulfhydrylase A, incorporate only small quantities of O-acetyl-L-serine into 1,2,4-triazole-1-alanine in vivo. In vitro studies, using purified preparations of O-acetylserine sulfhydrylase A, revealed greater losses of triazolylase activity than sulfhydrylase activity in the enzymes from both cysK mutants. Resistance to 1,2,4-triazole apparently can arise from mutations leading to a preferential loss of triazolylase activity or from mutations which diminish both activities to the extent that high concentrations of O-acetyl-L-serine and sulfide accumulate behind the sulfhydrylase reaction.", "contents": "Studies on the mechanism of inhibition of Salmonella typhimurium by 1,2,4-triazole. The inhibition of Salmonella typhimurium by 1,2,4-triazole appears to be mediated through an effect on L-cysteine biosynthesis. O-Acetylserine sulfhydrylase A, the final enzyme in the L-cysteine biosynthetic pathway, was found to catalyze a reaction (triazolylase) between O-acetyl-L-serine and 1,2,4-triazole, giving 1,2,4-triazole-1-alanine as a product. In wild type S. typhimurium grown on 4 mM 1,2,4-triazole, 97% of the total O-acetyl-L-serine synthesized in vivo is incorporated into 1,2,4-triazole-1-alanine. 1,2,4-triazole also significantly lowers the levels of several of the enzymes necessary for sulfate reduction. This effect is presumably due to the ability of the inhibitor to lower intracellular concentrations of O-acetyl-L-serine, an inducer of these enzymes. Inhibition of growth is probably caused by L-cysteine starvation, arising from the decreased availability of the L-cysteine precursors, sulfide and O-acetyl-L-serine. Two 1,2,4-triazole-resistant strains bearing mutations in cysK, the structural gene for O-acetylserine sulfhydrylase A, incorporate only small quantities of O-acetyl-L-serine into 1,2,4-triazole-1-alanine in vivo. In vitro studies, using purified preparations of O-acetylserine sulfhydrylase A, revealed greater losses of triazolylase activity than sulfhydrylase activity in the enzymes from both cysK mutants. Resistance to 1,2,4-triazole apparently can arise from mutations leading to a preferential loss of triazolylase activity or from mutations which diminish both activities to the extent that high concentrations of O-acetyl-L-serine and sulfide accumulate behind the sulfhydrylase reaction."}
{"id": "PMID:1100625", "title": "Outer membrane as a diffusion barrier in Salmonella typhimurium. Penetration of oligo- and polysaccharides into isolated outer membrane vesicles and cells with degraded peptidoglycan layer.", "content": "In Escherichia coli and Salmonella typhimurium, the cell wall that contains both the outer membrane layer and the peptidoglycan layer acts as a barrier of the molecular sieve type for the penetration of uncharged saccharides (G. Decad, T. Nakae, and H. Nikaido (1974) Fed. Proc. 33, 1240). Here we examined which of the layers of the cell wall limited the size of the penetrating molecules, by studying the penetration of saccharides into (a) cells whose peptidoglycan layer had been destroyed by lysozyme treatment or growth in the presence of penicillin and (b) isolated outer membrane vesicles. We found that peptidoglycan-defective cells were similar to intact, plasmolyzed cells in that they allowed a partial penetration of stachyose (molecular weight 666), but essentially excluded saccharides with molecular weights higher than 900 to 1000. We also found that the isolated outer membrane acted as a penetration barrier for saccharides. These observations led us to conclude that the outer membrane, rather than peptidoglycan, sets the size limit for the penetration of uncharged, hydrophilic molecules through the E. coli or S. typhimurium cell wall. The isolated outer membrane, however, had an exclusion limit much higher than that found in intact cells. This \"leakiness\" could be decreased either by the use of mutants producing extremely deficient lipopolysaccharide, or by trypsin treatment of the isolated membrane followed by heating and slow cooling in the presence of Mg2+. We feel that these observations are consistent with the hypothesis that the resealing of the ruptured outer membrane during the isolation procedure is often incomplete, and that cracks and holes thus generated are responsible for the \"leakiness\" of the isolated membrane vesicles.", "contents": "Outer membrane as a diffusion barrier in Salmonella typhimurium. Penetration of oligo- and polysaccharides into isolated outer membrane vesicles and cells with degraded peptidoglycan layer. In Escherichia coli and Salmonella typhimurium, the cell wall that contains both the outer membrane layer and the peptidoglycan layer acts as a barrier of the molecular sieve type for the penetration of uncharged saccharides (G. Decad, T. Nakae, and H. Nikaido (1974) Fed. Proc. 33, 1240). Here we examined which of the layers of the cell wall limited the size of the penetrating molecules, by studying the penetration of saccharides into (a) cells whose peptidoglycan layer had been destroyed by lysozyme treatment or growth in the presence of penicillin and (b) isolated outer membrane vesicles. We found that peptidoglycan-defective cells were similar to intact, plasmolyzed cells in that they allowed a partial penetration of stachyose (molecular weight 666), but essentially excluded saccharides with molecular weights higher than 900 to 1000. We also found that the isolated outer membrane acted as a penetration barrier for saccharides. These observations led us to conclude that the outer membrane, rather than peptidoglycan, sets the size limit for the penetration of uncharged, hydrophilic molecules through the E. coli or S. typhimurium cell wall. The isolated outer membrane, however, had an exclusion limit much higher than that found in intact cells. This \"leakiness\" could be decreased either by the use of mutants producing extremely deficient lipopolysaccharide, or by trypsin treatment of the isolated membrane followed by heating and slow cooling in the presence of Mg2+. We feel that these observations are consistent with the hypothesis that the resealing of the ruptured outer membrane during the isolation procedure is often incomplete, and that cracks and holes thus generated are responsible for the \"leakiness\" of the isolated membrane vesicles."}
{"id": "PMID:1100626", "title": "Inactivation of normal beta-D-galactosidase by antibodies to defective forms of the enzyme.", "content": "A counterpart of the antibody-mediated activation of genetically defective enzymes is reported here. Antibodies elicited by certain mutant forms of beta-D-galactosidase (EC 3.2.1.23) of Escherichia coli were found to inactivate the normal form of the enzyme. (Antibodies elicited by normal beta-D-galactosidase do not affect the enzyme's catalytic activity.) We present evidence that the inactivating antibodies are directed against one or a few determinants of the enzyme. The level of inactivation caused by the antibodies was independent of temperature below 25 degrees and increased with temperature above 25 degrees. The inactivation was proportional to the concentration of antiserum until a maximum level of 50% inactivation was reached. Antibodies capable of inactivating up to 87% of the activity were obtained after the antiserum was partially absorbed in an affinity column. This antibody preparation showed a 10-fold enrichment of inactivating antibodies over other antibodies direct against the enzyme. The antibody-mediated inactivation caused a reduction in the Vmax of beta-D-galactosidase without affecting the apparent Km of the enzyme. In contrast to antibodies to normal beta-D-galactosidase, inactivating antibodies changed the response of the enzyme to cations. To explain these results, we present a model in which there is a temperature-dependent equilibrium between two active forms of beta-D-galactosidase. Inactivation results from a conformational change induced by the binding of inactivating antibodies to only one of these two forms.", "contents": "Inactivation of normal beta-D-galactosidase by antibodies to defective forms of the enzyme. A counterpart of the antibody-mediated activation of genetically defective enzymes is reported here. Antibodies elicited by certain mutant forms of beta-D-galactosidase (EC 3.2.1.23) of Escherichia coli were found to inactivate the normal form of the enzyme. (Antibodies elicited by normal beta-D-galactosidase do not affect the enzyme's catalytic activity.) We present evidence that the inactivating antibodies are directed against one or a few determinants of the enzyme. The level of inactivation caused by the antibodies was independent of temperature below 25 degrees and increased with temperature above 25 degrees. The inactivation was proportional to the concentration of antiserum until a maximum level of 50% inactivation was reached. Antibodies capable of inactivating up to 87% of the activity were obtained after the antiserum was partially absorbed in an affinity column. This antibody preparation showed a 10-fold enrichment of inactivating antibodies over other antibodies direct against the enzyme. The antibody-mediated inactivation caused a reduction in the Vmax of beta-D-galactosidase without affecting the apparent Km of the enzyme. In contrast to antibodies to normal beta-D-galactosidase, inactivating antibodies changed the response of the enzyme to cations. To explain these results, we present a model in which there is a temperature-dependent equilibrium between two active forms of beta-D-galactosidase. Inactivation results from a conformational change induced by the binding of inactivating antibodies to only one of these two forms."}
{"id": "PMID:1100627", "title": "Action of the highly purified, membrane-bound enzyme phosphatidylserine decarboxylase Escherichia coli toward phosphatidylserine in mixed micelles and erythrocyte ghosts in the presence of surfactant.", "content": "Phosphatidylserine decarboxylase, Escheichia coli, was purified to near-homogeneity by the procedure of Dowhan, W., Wickner, W. T., and Kennedy, E. P. ((1974) J. Biol. Chem. 249, 3079-3084) and assayed by following the production of CO2 using gas chromatography. The purified enzyme has an absolute requirement for the surfactant Triton X-100. The function of Triton in the assay is evaluated and a kinetic scheme describing the action of this membrane-bound enzyme in the micellar system provided by the surfactant is presented. According to this scheme, the enzyme first binds to a mixed micelle, composed of phosphatidylserine and Triton, where the dissociation constant is KSA. The enzyme, now part of the mixed micelle, then binds the substrate phosphatidylserine in its active site and this binding is related to the Michaelis constant, KMB. KSA, expressed as the sum of the molar concentrations of Triton and phosphatidylserine, is about 0.04 M. KMB, expressed as the mole fraction of phosphatidylserine in the mixed micelles, is about 0.03. Phosphatidylserine decarboxylase activity toward phosphatidylserine in human erythrocyte ghosts was also determined. The amount of phsophatidylserine converted to phosphatidylethanolamine and CO2 was found to be related to the amount of phosphatidylserine solubilized from the membrane by Triton X-100. In the absence of Triton, no significant activity of the enzyme toward the ghosts was detected even after subjecting the ghosts to lyophilization, homogenization, or sonication.", "contents": "Action of the highly purified, membrane-bound enzyme phosphatidylserine decarboxylase Escherichia coli toward phosphatidylserine in mixed micelles and erythrocyte ghosts in the presence of surfactant. Phosphatidylserine decarboxylase, Escheichia coli, was purified to near-homogeneity by the procedure of Dowhan, W., Wickner, W. T., and Kennedy, E. P. ((1974) J. Biol. Chem. 249, 3079-3084) and assayed by following the production of CO2 using gas chromatography. The purified enzyme has an absolute requirement for the surfactant Triton X-100. The function of Triton in the assay is evaluated and a kinetic scheme describing the action of this membrane-bound enzyme in the micellar system provided by the surfactant is presented. According to this scheme, the enzyme first binds to a mixed micelle, composed of phosphatidylserine and Triton, where the dissociation constant is KSA. The enzyme, now part of the mixed micelle, then binds the substrate phosphatidylserine in its active site and this binding is related to the Michaelis constant, KMB. KSA, expressed as the sum of the molar concentrations of Triton and phosphatidylserine, is about 0.04 M. KMB, expressed as the mole fraction of phosphatidylserine in the mixed micelles, is about 0.03. Phosphatidylserine decarboxylase activity toward phosphatidylserine in human erythrocyte ghosts was also determined. The amount of phsophatidylserine converted to phosphatidylethanolamine and CO2 was found to be related to the amount of phosphatidylserine solubilized from the membrane by Triton X-100. In the absence of Triton, no significant activity of the enzyme toward the ghosts was detected even after subjecting the ghosts to lyophilization, homogenization, or sonication."}
{"id": "PMID:1100628", "title": "DNA repair synthesis dependent on the uvrA,B gene products in toluene-treated cells.", "content": "Ultraviolet irradiation of toluene-treated Escherichia coli causes an inhibition of replicative DNA synthesis. This is followed by the appearance of nonconservative DNA repair synthesis which does not require either the polymerase or 5' leads to 3' exonucleolytic activities of DNA polymerase I. The repair synthesis may be catalyzed by DNA polymerase III activity but does not require a functional DNA polymerase II. The ultraviolet-induced synthesis requires ATP and is dependent on a functional uvrA and uvrB gene product. However, other uvr gene products are not required for the synthesis. The recB function is also not required.", "contents": "DNA repair synthesis dependent on the uvrA,B gene products in toluene-treated cells. Ultraviolet irradiation of toluene-treated Escherichia coli causes an inhibition of replicative DNA synthesis. This is followed by the appearance of nonconservative DNA repair synthesis which does not require either the polymerase or 5' leads to 3' exonucleolytic activities of DNA polymerase I. The repair synthesis may be catalyzed by DNA polymerase III activity but does not require a functional DNA polymerase II. The ultraviolet-induced synthesis requires ATP and is dependent on a functional uvrA and uvrB gene product. However, other uvr gene products are not required for the synthesis. The recB function is also not required."}
{"id": "PMID:1100629", "title": "The in vitro translation of a terminating signal by a single Escherichia coli ribosome. The fate of the subunits.", "content": "A complex was isolated containing one 70 S ribosome bound to the RNA of the f2 bacteriophage in the region of the coat gene. Either the f2 RNA and 70 S ribosome or both ribosomal subunits in this complex were differentially labeled with tritiated lysine or phosphorous 32 RNA. Incubation of this complex in a ribosome-free protein-synthesizing system lacking initiating factor and appreciable nuclease activities allowed the synthesis of coat protein and subsequent termination. Using this techique, it was found that termination resulted in the release of ribosome as subunits. Intermediate in this process is a transient 46 S complex composed of the f2 RNA and 30 S ribosomal subunit, presumably the result of the initial release of the 50 S ribosomal subunit. This 46 S complex subsequently breaks down to a free f2 RNA and 30 S ribosomal subunit. The rate of this latter process is faster in the presence of crude initiating factors. These results are discussed in terms of the mechanism of termination and reading of the intercistronic region.", "contents": "The in vitro translation of a terminating signal by a single Escherichia coli ribosome. The fate of the subunits. A complex was isolated containing one 70 S ribosome bound to the RNA of the f2 bacteriophage in the region of the coat gene. Either the f2 RNA and 70 S ribosome or both ribosomal subunits in this complex were differentially labeled with tritiated lysine or phosphorous 32 RNA. Incubation of this complex in a ribosome-free protein-synthesizing system lacking initiating factor and appreciable nuclease activities allowed the synthesis of coat protein and subsequent termination. Using this techique, it was found that termination resulted in the release of ribosome as subunits. Intermediate in this process is a transient 46 S complex composed of the f2 RNA and 30 S ribosomal subunit, presumably the result of the initial release of the 50 S ribosomal subunit. This 46 S complex subsequently breaks down to a free f2 RNA and 30 S ribosomal subunit. The rate of this latter process is faster in the presence of crude initiating factors. These results are discussed in terms of the mechanism of termination and reading of the intercistronic region."}
{"id": "PMID:1100630", "title": "The isolation of RNA homologous to the genetic control elements of the lactose operon.", "content": "A sequential DNA-RNA hybridization procedure is described whereby RNA homologous to a target DNA region 35 to 140 base pairs in length can be purified up to 6700-fold from a complex in vitro transcript to a homogeneity sufficient for sequence analysis. Requirements for the procedure include: (a) uniform transcription over the target DNA region in vitro; (b) specialized transducing phages which carry genetic deletions defining the target region on either side; and (c) specialized transducing phages which carry the target DNA in opposite orientations. These requirements have been met for the genetic control region (promoter, operator) of the lactose operon of Escherichia coli, to which the method was applied. The procedure is independent of the activity of the genetic control signals under study and can therefore be applied without modification to the study of point mutations introduced into the template.", "contents": "The isolation of RNA homologous to the genetic control elements of the lactose operon. A sequential DNA-RNA hybridization procedure is described whereby RNA homologous to a target DNA region 35 to 140 base pairs in length can be purified up to 6700-fold from a complex in vitro transcript to a homogeneity sufficient for sequence analysis. Requirements for the procedure include: (a) uniform transcription over the target DNA region in vitro; (b) specialized transducing phages which carry genetic deletions defining the target region on either side; and (c) specialized transducing phages which carry the target DNA in opposite orientations. These requirements have been met for the genetic control region (promoter, operator) of the lactose operon of Escherichia coli, to which the method was applied. The procedure is independent of the activity of the genetic control signals under study and can therefore be applied without modification to the study of point mutations introduced into the template."}
{"id": "PMID:1100631", "title": "Purification of Escherichia coli endonuclease specific for apurinic sites in DNA.", "content": "The endonuclease specific for apurinic sites in DNA has been isolated from Escherichia coli B41 as a pure monomeric protein of 32,000 daltons. The enzyme hydrolyzes a phosphodiester bond near the apurinic sites in double-stranded DNA; it does not hydrolyze untreated DNA and its action on alkylated DNA is restricted to the apurinic sites always present. This enzyme is not endonuclease II which is most probably a mixture of two enzymes, one a glycosidase (Kirtikar, D. M., and Goldthwait, D. A. (1974) Proc. Natl. Acad. Sci. U. S. A. 71, 2022-2026), the other an endonuclease for apurinic sites which is the enzyme isolated in this work.", "contents": "Purification of Escherichia coli endonuclease specific for apurinic sites in DNA. The endonuclease specific for apurinic sites in DNA has been isolated from Escherichia coli B41 as a pure monomeric protein of 32,000 daltons. The enzyme hydrolyzes a phosphodiester bond near the apurinic sites in double-stranded DNA; it does not hydrolyze untreated DNA and its action on alkylated DNA is restricted to the apurinic sites always present. This enzyme is not endonuclease II which is most probably a mixture of two enzymes, one a glycosidase (Kirtikar, D. M., and Goldthwait, D. A. (1974) Proc. Natl. Acad. Sci. U. S. A. 71, 2022-2026), the other an endonuclease for apurinic sites which is the enzyme isolated in this work."}
{"id": "PMID:1100632", "title": "A comparison of iliac marrow and biodegradable ceramic in periodontal defects.", "content": "Defects were created in the tooth-supporting bone of six beagle dogs. Biodegradable tricalcium phosphate was compared to cancellous marrow as a treatment method. The findings indicated that ankylosis and resorption of the tooth can occur when using marrow. The ceramic-filled defects healed slower; but the material itself was well tolerated by the tissues, initiated new bone formation, filled in the defect, and has good potential for the treatment of advanced periodontitis.", "contents": "A comparison of iliac marrow and biodegradable ceramic in periodontal defects. Defects were created in the tooth-supporting bone of six beagle dogs. Biodegradable tricalcium phosphate was compared to cancellous marrow as a treatment method. The findings indicated that ankylosis and resorption of the tooth can occur when using marrow. The ceramic-filled defects healed slower; but the material itself was well tolerated by the tissues, initiated new bone formation, filled in the defect, and has good potential for the treatment of advanced periodontitis."}
{"id": "PMID:1100633", "title": "Thermodynamic considerations of the setting reaction in Ag3Sn amalgams.", "content": "The setting reaction of Ag3Sn amalgams may be thought of as a liquid phase sintering problem in which the reactants seek to form products with the lowest thermodynamic potential. We discuss the thermodynamic and kinetic considerations of this reaction. The lowering of free energy has been given as the reason for the formation of the gamma1, gamma2,and beta1 phases and a qualitative reaction coordinate diagram has been hypothesized. The barrier height for the formation of the beta1 phase has been determined to be 137 kJ/mol.", "contents": "Thermodynamic considerations of the setting reaction in Ag3Sn amalgams. The setting reaction of Ag3Sn amalgams may be thought of as a liquid phase sintering problem in which the reactants seek to form products with the lowest thermodynamic potential. We discuss the thermodynamic and kinetic considerations of this reaction. The lowering of free energy has been given as the reason for the formation of the gamma1, gamma2,and beta1 phases and a qualitative reaction coordinate diagram has been hypothesized. The barrier height for the formation of the beta1 phase has been determined to be 137 kJ/mol."}
{"id": "PMID:1100634", "title": "Use of PMMA in expansion dental implants.", "content": "The Polymer Expansion Implant System, which is a system for the substitution of missing dental roots in natural or surgical alveolus, has been developed by the author. The polymer used is poly (methyl methacrylate) (PMMA). A material is sought that has three of the basic physical and mechanical qualities of human dentine: low modulus of elasticity, thermal and electrical passiveness, and ideal porosity. In addition it should be resistant, easily worked, biocompatible, and of low cost. Of the biomedical polymers, PMMA has these qualities, and it can be used as a substitute for dental roots. The design of bodies to be implanted as substitutes for dental roots requires: anatomical measurements of the maxilla and sockets; methods of retention by the bone tissue; and methods of insertion. The formation of lamina dura around an implanted body is a reply of the organism to the forces acting on it and a demonstration of the good interaction of the porosity of the material, the peri-implant collagenous fibers and bone. X-rays that demonstrate the behavior of bone to a PMMA implant are shown.", "contents": "Use of PMMA in expansion dental implants. The Polymer Expansion Implant System, which is a system for the substitution of missing dental roots in natural or surgical alveolus, has been developed by the author. The polymer used is poly (methyl methacrylate) (PMMA). A material is sought that has three of the basic physical and mechanical qualities of human dentine: low modulus of elasticity, thermal and electrical passiveness, and ideal porosity. In addition it should be resistant, easily worked, biocompatible, and of low cost. Of the biomedical polymers, PMMA has these qualities, and it can be used as a substitute for dental roots. The design of bodies to be implanted as substitutes for dental roots requires: anatomical measurements of the maxilla and sockets; methods of retention by the bone tissue; and methods of insertion. The formation of lamina dura around an implanted body is a reply of the organism to the forces acting on it and a demonstration of the good interaction of the porosity of the material, the peri-implant collagenous fibers and bone. X-rays that demonstrate the behavior of bone to a PMMA implant are shown."}
{"id": "PMID:1100635", "title": "A failed vitallium/stainless steel total hip replacement: a case report with histological and metallurgical examination.", "content": "A case of a failed total hip replacement consisting of a Vitallium hip socket and a stainless steel femoral head prosthesis is presented. The mixed-metal combination and inadequate design resulted in severe abrasive wear and fretting corrosion of the bearing surface, and the accumulation of stainless steel wear debris and corrosion products in the periarticular tissues was extensive. The tissue response was primarily fibrotic scarring with severe histiocytic and mild foreign body giant cell reaction. Despite the massive accumulation of wear debris and corrosion products, however, there was minimal chronic inflammation and no acute inflammation.", "contents": "A failed vitallium/stainless steel total hip replacement: a case report with histological and metallurgical examination. A case of a failed total hip replacement consisting of a Vitallium hip socket and a stainless steel femoral head prosthesis is presented. The mixed-metal combination and inadequate design resulted in severe abrasive wear and fretting corrosion of the bearing surface, and the accumulation of stainless steel wear debris and corrosion products in the periarticular tissues was extensive. The tissue response was primarily fibrotic scarring with severe histiocytic and mild foreign body giant cell reaction. Despite the massive accumulation of wear debris and corrosion products, however, there was minimal chronic inflammation and no acute inflammation."}
{"id": "PMID:1100640", "title": "[Renal preservation: comparative effectiveness of 2 simple perfusion technics].", "content": "The authors compare the efficacy of two perfusates, Collins' solution and Perfudex, for conservation of kidneys for short periods by perfusion and hypothermic immersion. 22 dog kidneys, removed by lombotomy, were preserved for 24 hours with one or other of these solutions and then re-implanted into their host. Radio-isotopic renography, the uptake of Hg 197 Cl3 and a hippuran renogram, used to assess the function of the transplants, suggested the functional superiority of kidneys preserved with Collins solutions. Histological controls carried out beyond the 10 th post-operative week, confirmed these results. The correlations observed between these various findings also assessed the reliability of radio-isotope examinations in the exploration of renal function.", "contents": "[Renal preservation: comparative effectiveness of 2 simple perfusion technics]. The authors compare the efficacy of two perfusates, Collins' solution and Perfudex, for conservation of kidneys for short periods by perfusion and hypothermic immersion. 22 dog kidneys, removed by lombotomy, were preserved for 24 hours with one or other of these solutions and then re-implanted into their host. Radio-isotopic renography, the uptake of Hg 197 Cl3 and a hippuran renogram, used to assess the function of the transplants, suggested the functional superiority of kidneys preserved with Collins solutions. Histological controls carried out beyond the 10 th post-operative week, confirmed these results. The correlations observed between these various findings also assessed the reliability of radio-isotope examinations in the exploration of renal function."}
{"id": "PMID:1100643", "title": "Effect of prolonged LH-releasing hormone administration on gonadotropin response in patients with hypothalamic and pituitary tumors.", "content": "The effect of prolonged administration of synthetic LH-RH (400 mug daily im for 5 days) on gonadotropin response to LH-RH (100 mug single injection iv) was compared in 13 patients with pituitary tumors or suprasellar tumors. No patients with pituitary tumors exhibited an augmented response on serum FSH and LH after prolonged LH-RH administration. A paradoxical fall in response to LH-RH test occurred in 3 patients with pituitary tumors and in a patient with hypothalamic tumor in whom gonadotropin responses were normal on the first LH-RH test. On the other hand, 2 patients with suprasellar tumors with a low response to the initial LH-RH test showed significant increase on a second LH-RHtest after consecutive LH-RH administration. Consecutive administration of LH-RH may help to distinguish between the hypogonadism of pituitary origin and that of hypothalamic origin when the initial LH response to a single dose of LH-RH is subnormal.", "contents": "Effect of prolonged LH-releasing hormone administration on gonadotropin response in patients with hypothalamic and pituitary tumors. The effect of prolonged administration of synthetic LH-RH (400 mug daily im for 5 days) on gonadotropin response to LH-RH (100 mug single injection iv) was compared in 13 patients with pituitary tumors or suprasellar tumors. No patients with pituitary tumors exhibited an augmented response on serum FSH and LH after prolonged LH-RH administration. A paradoxical fall in response to LH-RH test occurred in 3 patients with pituitary tumors and in a patient with hypothalamic tumor in whom gonadotropin responses were normal on the first LH-RH test. On the other hand, 2 patients with suprasellar tumors with a low response to the initial LH-RH test showed significant increase on a second LH-RHtest after consecutive LH-RH administration. Consecutive administration of LH-RH may help to distinguish between the hypogonadism of pituitary origin and that of hypothalamic origin when the initial LH response to a single dose of LH-RH is subnormal."}
{"id": "PMID:1100644", "title": "Pituitary response to synthetic luteinizing hormone-releasing hormone in patients with Turner's syndrone.", "content": "Synthetic luteinizing hormone-releasing hormone (LHRH) was administered intravenously in a dose of 25 mug to 9 patients with Turner's syndrome of 45,XO karyotypes, 5 normal menstruating women, and 5 postmenopausal women. In patients with Turner's syndrome, the basal serum LH level was higher than that of the normal subjects and lower than that of postmenopausal women. The LH response to LHRH, when expressed as a percent of control, was comparable to that of the normal luteal phase and significantly higher than that of postmenopausal women. The basal FSH level was more elevated than the basal LH level. The FSH response to LHRH, when expressed as a percent of control, was similar to that of postmenopausal women and lower than that of normal subjects. The results suggest that pituitary function is preserved in patients with Turner's syndrome. I interpret the pituitary responsiveness and/or reserve of patients with Turner's syndrome as larger than that of postmenopausal women. A greater response of LH than of FSH to LHRH was observed in this study and is contrary to findings reported by others on teenage patients with Turner's syndrome. This suggests that the sensitivity of gonadotropins changes with age in Turner's syndrome.", "contents": "Pituitary response to synthetic luteinizing hormone-releasing hormone in patients with Turner's syndrone. Synthetic luteinizing hormone-releasing hormone (LHRH) was administered intravenously in a dose of 25 mug to 9 patients with Turner's syndrome of 45,XO karyotypes, 5 normal menstruating women, and 5 postmenopausal women. In patients with Turner's syndrome, the basal serum LH level was higher than that of the normal subjects and lower than that of postmenopausal women. The LH response to LHRH, when expressed as a percent of control, was comparable to that of the normal luteal phase and significantly higher than that of postmenopausal women. The basal FSH level was more elevated than the basal LH level. The FSH response to LHRH, when expressed as a percent of control, was similar to that of postmenopausal women and lower than that of normal subjects. The results suggest that pituitary function is preserved in patients with Turner's syndrome. I interpret the pituitary responsiveness and/or reserve of patients with Turner's syndrome as larger than that of postmenopausal women. A greater response of LH than of FSH to LHRH was observed in this study and is contrary to findings reported by others on teenage patients with Turner's syndrome. This suggests that the sensitivity of gonadotropins changes with age in Turner's syndrome."}
{"id": "PMID:1100645", "title": "Detection of bacteriuria by luciferase assay of adenosine triphosphate.", "content": "A selective method for distinguishing bacterial and nonbacterial adenosine triphosphate (ATP) in clinical bacteriological specimens was studied. The method involved incubation of samples with the detergent Triton X-100 and the ATP-hydrolyzing enzyme apyrase. The incubation selectively destroyed ATP in suspensions of various human cells while not affecting the ATP content in microbial cells. ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay. Application of the method to 469 clinical urine specimens showed that the ATP level after treatment with Triton/apyrase was correlated to bacterial counts and that the sensitivity of the assay was sufficient for the detection of 10(5) bacteria/ml. The ATP levels per bacterial cell remaining in the urine specimen after treatment with Triton/apyrase were close to values observed in laboratory-grown cultures. The specificity and sensitivity of the luciferase assay for the detection of urinary bacteria and its possible use as a bacteriuria screening method are discussed.", "contents": "Detection of bacteriuria by luciferase assay of adenosine triphosphate. A selective method for distinguishing bacterial and nonbacterial adenosine triphosphate (ATP) in clinical bacteriological specimens was studied. The method involved incubation of samples with the detergent Triton X-100 and the ATP-hydrolyzing enzyme apyrase. The incubation selectively destroyed ATP in suspensions of various human cells while not affecting the ATP content in microbial cells. ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay. Application of the method to 469 clinical urine specimens showed that the ATP level after treatment with Triton/apyrase was correlated to bacterial counts and that the sensitivity of the assay was sufficient for the detection of 10(5) bacteria/ml. The ATP levels per bacterial cell remaining in the urine specimen after treatment with Triton/apyrase were close to values observed in laboratory-grown cultures. The specificity and sensitivity of the luciferase assay for the detection of urinary bacteria and its possible use as a bacteriuria screening method are discussed."}
{"id": "PMID:1100646", "title": "Unusual Enterobacteriaceae: a Salmonella cubana that is urease positive.", "content": "This is the first report of a naturally occurring Salmonella that is urea positive. The strain was identified as Salmonella cubana and it was typical in all biochemical, serological, and bacteriophage reactions, except that is produced urease strongly.", "contents": "Unusual Enterobacteriaceae: a Salmonella cubana that is urease positive. This is the first report of a naturally occurring Salmonella that is urea positive. The strain was identified as Salmonella cubana and it was typical in all biochemical, serological, and bacteriophage reactions, except that is produced urease strongly."}
{"id": "PMID:1100641", "title": "[Comparative experimental study of inverting and everting sutures in the colon].", "content": "The authors carried out 88 sutures of the ascending colon in Wistar rats, 44 in inversion and the remainder in eversion. The suture material used was monofilament 9/0 nylon. The intestinal diameter was measured using an instrument and the healing process studied by means of the following parameters: granulocytes, oedema, fibrin, fibroblasts and re-epithelialisation of the mucosa, on histology. The results prove that inverted sutures heal within 10 days, whereas everted sutures are not healed after 17 days. Furthermore, we demonstrated the fact that, after 72 hours, the intestinal caliber above the suture showed no appreciable differences compared with that of the normal colon.", "contents": "[Comparative experimental study of inverting and everting sutures in the colon]. The authors carried out 88 sutures of the ascending colon in Wistar rats, 44 in inversion and the remainder in eversion. The suture material used was monofilament 9/0 nylon. The intestinal diameter was measured using an instrument and the healing process studied by means of the following parameters: granulocytes, oedema, fibrin, fibroblasts and re-epithelialisation of the mucosa, on histology. The results prove that inverted sutures heal within 10 days, whereas everted sutures are not healed after 17 days. Furthermore, we demonstrated the fact that, after 72 hours, the intestinal caliber above the suture showed no appreciable differences compared with that of the normal colon."}
{"id": "PMID:1100647", "title": "Evaluation of the Bactrol Disks, a set of quality control cultures.", "content": "A set of commercially available quality control cultures was evaluated. These cultures were found to be an acceptable alternate to lyophilized or continuously subcultured cultures for a quality control program.", "contents": "Evaluation of the Bactrol Disks, a set of quality control cultures. A set of commercially available quality control cultures was evaluated. These cultures were found to be an acceptable alternate to lyophilized or continuously subcultured cultures for a quality control program."}
{"id": "PMID:1100648", "title": "Rapid hippurate hydrolysis method for presumptive identification of group B streptococci.", "content": "A rapid test to detect the hydrolysis of sodium hippurate by beta-hemolytic streptococci within 2 h was developed. All group B streptococci tested were positive using this method and all other groups were negative.", "contents": "Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. A rapid test to detect the hydrolysis of sodium hippurate by beta-hemolytic streptococci within 2 h was developed. All group B streptococci tested were positive using this method and all other groups were negative."}
{"id": "PMID:1100649", "title": "Comparison of four methods for determining nitrate utilization by cryptococci.", "content": "This study evaluated the following methods for determining nitrate utilization: Wickerham broth, a special nitrate broth, Delft plate, and nitrate strip. With 236 isolates of cryptococci as test organisms, the special nitrate broth method gave 99% correct results and the Wickerham broth method gave 98%. The nitrate strip and Delft plate methods gave correct results in 94 and 86% of tests, respectively. The special nitrate broth method is judged superior because it provides accurate results within 48 h, compared to 14 days with the Wickerham broth method.", "contents": "Comparison of four methods for determining nitrate utilization by cryptococci. This study evaluated the following methods for determining nitrate utilization: Wickerham broth, a special nitrate broth, Delft plate, and nitrate strip. With 236 isolates of cryptococci as test organisms, the special nitrate broth method gave 99% correct results and the Wickerham broth method gave 98%. The nitrate strip and Delft plate methods gave correct results in 94 and 86% of tests, respectively. The special nitrate broth method is judged superior because it provides accurate results within 48 h, compared to 14 days with the Wickerham broth method."}
{"id": "PMID:1100650", "title": "Effect of acetone fixation on infectivity and antigenicity of respiratory syncytial virus and adenovirus in the fluorescent antibody test.", "content": "An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for periods ranging from 30 s to 7 days. Treatment for 10 min was sufficient to inactivate respiratory syncytial virus, whereas infectious adenovirus type 5 could be isolated from cells immersed in acetone for 7 days. There was a gradual reduction, to 50% of that observed at 30 s, in the intensity of fluorescent antibody staining of both viruses with increasing fixation time, but no significant decreases in fluorescent antibody end-point titers of antisera to either virus were observed.", "contents": "Effect of acetone fixation on infectivity and antigenicity of respiratory syncytial virus and adenovirus in the fluorescent antibody test. An investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. Viruses were allowed to replicate in HEp-2 cells, and the cells were then fixed in acetone at 5 C for periods ranging from 30 s to 7 days. Treatment for 10 min was sufficient to inactivate respiratory syncytial virus, whereas infectious adenovirus type 5 could be isolated from cells immersed in acetone for 7 days. There was a gradual reduction, to 50% of that observed at 30 s, in the intensity of fluorescent antibody staining of both viruses with increasing fixation time, but no significant decreases in fluorescent antibody end-point titers of antisera to either virus were observed."}
{"id": "PMID:1100651", "title": "Standardization and evaluation of the CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material.", "content": "Primary cultures of clinical material were screened for the presence of colonies suspected of being Streptococcus agalactiae (Lancefield group B). Sixty-three such cultures and 108 other isolates of beta-hemolytic streptococci (groups A, C, and G), encountered during the first 3 months of the investigation, were studied by Lancefield grouping, sodium hippurate hydrolysis, and a standardized CAMP test. All streptococci were inoculated perpendicularly to streaks of a beta-toxin-producing staphylococcus on sheep blood agar plates and incubated aerobically in a candle jar and anaerobically at 37 C. Plates were examined after 5 to 6 and 18 h of incubation. The production of a distinct \"arrowhead\" of hemolysis was indicative of a positive CAMP reaction. All group B streptococci produced a positive CAMP reaction in the candle jar or anaerobically, usually within 5 to 6 h, and aerobically after 18 h of incubation. All group A streptococci produced a positive reaction only under anaerobic conditions. Groups C and G streptococci were negative under all atmospheres. The CAMP reaction is a prompt and reliable procedure for the presumptive identification of group B streptococci when a candle jar atmosphere is used during incubation.", "contents": "Standardization and evaluation of the CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material. Primary cultures of clinical material were screened for the presence of colonies suspected of being Streptococcus agalactiae (Lancefield group B). Sixty-three such cultures and 108 other isolates of beta-hemolytic streptococci (groups A, C, and G), encountered during the first 3 months of the investigation, were studied by Lancefield grouping, sodium hippurate hydrolysis, and a standardized CAMP test. All streptococci were inoculated perpendicularly to streaks of a beta-toxin-producing staphylococcus on sheep blood agar plates and incubated aerobically in a candle jar and anaerobically at 37 C. Plates were examined after 5 to 6 and 18 h of incubation. The production of a distinct \"arrowhead\" of hemolysis was indicative of a positive CAMP reaction. All group B streptococci produced a positive CAMP reaction in the candle jar or anaerobically, usually within 5 to 6 h, and aerobically after 18 h of incubation. All group A streptococci produced a positive reaction only under anaerobic conditions. Groups C and G streptococci were negative under all atmospheres. The CAMP reaction is a prompt and reliable procedure for the presumptive identification of group B streptococci when a candle jar atmosphere is used during incubation."}
{"id": "PMID:1100652", "title": "Rapid identification of group B streptococci by counterimmunoelectrophoresis.", "content": "Beta-hemolytic streptococcal isolates have been examined by counterimmunoelectrophoresis (CIE) with group B antiserum to determine whether this techinque is of value in the rapid identification of group B strains. Ninety stock cultures and 100 clinical isolates of beta-hemolytic streptococci including representatives of groups A, D, C, G, and B were inoculated into Todd-Hewitt broth; after incubation at 37 C for 1, 2, 3, and 4 h, aliquots of the whole broth cultures were removed and tested by CIE. Antigen was not regularly detected in the 1-, 2-, and 3-h samples, but after 4 h all 126 group B streptococcal strains identified by the capillary precipitin reaction gave CIE precipitin bands with group B antiserum. None of the 58 non-group B strains gave precipitin reactions with this antiserum. Cerebrospinal fluid from an infant with group B streptococcal meningitis and peritoneal fluid from a patient with group B streptococcal peritonitis had free group B antigen detected by the CIE technique. CIE of broth cultures and direct body fluids appears to be a rapid and sensitive method for the identification of group B streptococcal strains.", "contents": "Rapid identification of group B streptococci by counterimmunoelectrophoresis. Beta-hemolytic streptococcal isolates have been examined by counterimmunoelectrophoresis (CIE) with group B antiserum to determine whether this techinque is of value in the rapid identification of group B strains. Ninety stock cultures and 100 clinical isolates of beta-hemolytic streptococci including representatives of groups A, D, C, G, and B were inoculated into Todd-Hewitt broth; after incubation at 37 C for 1, 2, 3, and 4 h, aliquots of the whole broth cultures were removed and tested by CIE. Antigen was not regularly detected in the 1-, 2-, and 3-h samples, but after 4 h all 126 group B streptococcal strains identified by the capillary precipitin reaction gave CIE precipitin bands with group B antiserum. None of the 58 non-group B strains gave precipitin reactions with this antiserum. Cerebrospinal fluid from an infant with group B streptococcal meningitis and peritoneal fluid from a patient with group B streptococcal peritonitis had free group B antigen detected by the CIE technique. CIE of broth cultures and direct body fluids appears to be a rapid and sensitive method for the identification of group B streptococcal strains."}
{"id": "PMID:1100653", "title": "Pagano-Levin Candida test medium: evaluation using vaginal samples.", "content": "Since 1958 pagano-Levin medium (PLM) has been used as an aid in the identification of Candida albicans. However, no statistical analysis of its effectiveness based on large number of random human specimens has been reported. The present study compared PLM (now called Candida test) with Sabouraud's plus antimicrobials and Littman's ox-gall agar without antimicrobials. Of 500 random vaginal samples 24.8 were true positives, 71.9% were true negatives, 2.2% were false positives, and 1.2% were false negatives on PLM. If only samples identified as C. albicans were considered, 95.4% were true positives and 4.6% were false negatives. PLM did not inhibit C. albicans from the vagina. No one medium was found superior to the others for the purpose of isolating and identifying C. albicans. Of the five strains of C. parapsilosis, the only other Candida species isolated, all the samples grew on PLM but most were inhibited on Sabouraud's medium plus antimicrobials.", "contents": "Pagano-Levin Candida test medium: evaluation using vaginal samples. Since 1958 pagano-Levin medium (PLM) has been used as an aid in the identification of Candida albicans. However, no statistical analysis of its effectiveness based on large number of random human specimens has been reported. The present study compared PLM (now called Candida test) with Sabouraud's plus antimicrobials and Littman's ox-gall agar without antimicrobials. Of 500 random vaginal samples 24.8 were true positives, 71.9% were true negatives, 2.2% were false positives, and 1.2% were false negatives on PLM. If only samples identified as C. albicans were considered, 95.4% were true positives and 4.6% were false negatives. PLM did not inhibit C. albicans from the vagina. No one medium was found superior to the others for the purpose of isolating and identifying C. albicans. Of the five strains of C. parapsilosis, the only other Candida species isolated, all the samples grew on PLM but most were inhibited on Sabouraud's medium plus antimicrobials."}
{"id": "PMID:1100654", "title": "Serotyping and biotyping of 160 Escherichia coli strains: comparative study.", "content": "One hundred and sixty Escherichia coli strains were serotyped and biotyped. Serotyping revealed 68 different types, with 25 strains not typable. Biotyping was possible in all strains but revealed 55 different types. One biotype could be subdivided into 35 different serotypes, indicating that for this biotype the series of biochemical and fermentation reactions could be extended for further differentiation.", "contents": "Serotyping and biotyping of 160 Escherichia coli strains: comparative study. One hundred and sixty Escherichia coli strains were serotyped and biotyped. Serotyping revealed 68 different types, with 25 strains not typable. Biotyping was possible in all strains but revealed 55 different types. One biotype could be subdivided into 35 different serotypes, indicating that for this biotype the series of biochemical and fermentation reactions could be extended for further differentiation."}
{"id": "PMID:1100655", "title": "Sensitivity of bhk-21 cells supplemented with diethylaminoethyl-dextran for detection of street rabies virus in saliva samples.", "content": "A tissue culture system for detecting rabies virus from saliva samples of suspected animals was developed and compared to suckling mouse inoculation. Swab samples were obtained from the mouth of the animal heads received for rabies diagnosis; these swabs were submerged in maintenance medium. The maintenance medium was inoculated intracerebrally into suckling mice and onto BHK-21 cells with diethylaminoethyl (DEAE)-dextran (BHK/DEAE) and without (BHK). Rabies immunofluorescence was performed on the brain of the mice dying during the observation period and also on both tissue culture systems every day after infection. The BHK-DEAE system detected 28 positive samples obtained from 48 rabid animals and the BHK system detected 18. By suckling mouse inoculation only 11 of the same positive samples were detected. A total of 90 samples was studied by the three methods. Rabies virus was detected by the tissue culture methods earlier than by suckling mouse inoculation. The BHK-DEAE method was an economic and fast method for rabies virus detection in saliva samples, which could be used for ecological and pathogenesis studies, as well for rabies diagnosis before the death of the suspected animal.", "contents": "Sensitivity of bhk-21 cells supplemented with diethylaminoethyl-dextran for detection of street rabies virus in saliva samples. A tissue culture system for detecting rabies virus from saliva samples of suspected animals was developed and compared to suckling mouse inoculation. Swab samples were obtained from the mouth of the animal heads received for rabies diagnosis; these swabs were submerged in maintenance medium. The maintenance medium was inoculated intracerebrally into suckling mice and onto BHK-21 cells with diethylaminoethyl (DEAE)-dextran (BHK/DEAE) and without (BHK). Rabies immunofluorescence was performed on the brain of the mice dying during the observation period and also on both tissue culture systems every day after infection. The BHK-DEAE system detected 28 positive samples obtained from 48 rabid animals and the BHK system detected 18. By suckling mouse inoculation only 11 of the same positive samples were detected. A total of 90 samples was studied by the three methods. Rabies virus was detected by the tissue culture methods earlier than by suckling mouse inoculation. The BHK-DEAE method was an economic and fast method for rabies virus detection in saliva samples, which could be used for ecological and pathogenesis studies, as well for rabies diagnosis before the death of the suspected animal."}
{"id": "PMID:1100656", "title": "Suitability of peracetic acid for sterilization of media for mycoplasma cultures.", "content": "The utility of peracetic acid for sterilization of serum and yeast extract additions to mycoplasma medium was studied by culturing six Mycoplasma species. Culture media containing additions that had been sterilized with peracetic acid proved to be as good as filtered components. The use of 0.05 to 0.1% peracetic acid is recommended to sterilize the serum and yeast extract additions since savings in time and equipment can be accomplished.", "contents": "Suitability of peracetic acid for sterilization of media for mycoplasma cultures. The utility of peracetic acid for sterilization of serum and yeast extract additions to mycoplasma medium was studied by culturing six Mycoplasma species. Culture media containing additions that had been sterilized with peracetic acid proved to be as good as filtered components. The use of 0.05 to 0.1% peracetic acid is recommended to sterilize the serum and yeast extract additions since savings in time and equipment can be accomplished."}
{"id": "PMID:1100657", "title": "Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody.", "content": "A simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens has been devised as a screening test for antibody in human sera. The test differs from our standard procedure by amalgamating 15 different immunotypes into nine antigen pools, by using only three serum dilutions, and by dropping use of duplicate slides. The screening test could be performed on at least six times as many sera as the standard test for a given unit of effort. It was shown to have a sensitivity equal to the standard test and an ability to determine specific immunotype on the basis of antibody pattern (72%) only slightly reduced from the standard test (84%). The screening test was carried out on 876 patients from different population groups in the Seattle area. The tests showed that antibody was present frequently in persons attending venereal disease clinics (60%) and commonly (25%) in a group of adults without venereal disease. Nine percent of children under 15 years of age tested had antibody; the significance of this finding is unknown.", "contents": "Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody. A simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens has been devised as a screening test for antibody in human sera. The test differs from our standard procedure by amalgamating 15 different immunotypes into nine antigen pools, by using only three serum dilutions, and by dropping use of duplicate slides. The screening test could be performed on at least six times as many sera as the standard test for a given unit of effort. It was shown to have a sensitivity equal to the standard test and an ability to determine specific immunotype on the basis of antibody pattern (72%) only slightly reduced from the standard test (84%). The screening test was carried out on 876 patients from different population groups in the Seattle area. The tests showed that antibody was present frequently in persons attending venereal disease clinics (60%) and commonly (25%) in a group of adults without venereal disease. Nine percent of children under 15 years of age tested had antibody; the significance of this finding is unknown."}
{"id": "PMID:1100658", "title": "Identification of streptococci: serogrouping by immunofluorescence.", "content": "This paper deals with the fluorescent antibody (FA) method for identifying six commonly occuring and two rare groups of streptococci by using commercially prepared (Difco) conjugates. We have shown that group-specific FA produced frequent cross-reactions with heterologous groups of organisms. These reactions varied with different strains of the same serogroup. Nonetheless, there was distinct overall patterns in the intensity and appearance of the homologous and heterologous reactions. When monitored by the precipitin test with Rantz and Randall extracts, these patterns led to the correct identification of 90 to 100% of specimens of serogroup A, B, C, and G streptococci. Many members of groups D and F also showed distinctive reaction patterns. However, there was a significant number of strains of both groups D and F that either failed to strain or stained poorly with the homologous conjugate. As a result, the identification of these serogroups by FA was less reliable.", "contents": "Identification of streptococci: serogrouping by immunofluorescence. This paper deals with the fluorescent antibody (FA) method for identifying six commonly occuring and two rare groups of streptococci by using commercially prepared (Difco) conjugates. We have shown that group-specific FA produced frequent cross-reactions with heterologous groups of organisms. These reactions varied with different strains of the same serogroup. Nonetheless, there was distinct overall patterns in the intensity and appearance of the homologous and heterologous reactions. When monitored by the precipitin test with Rantz and Randall extracts, these patterns led to the correct identification of 90 to 100% of specimens of serogroup A, B, C, and G streptococci. Many members of groups D and F also showed distinctive reaction patterns. However, there was a significant number of strains of both groups D and F that either failed to strain or stained poorly with the homologous conjugate. As a result, the identification of these serogroups by FA was less reliable."}
{"id": "PMID:1100659", "title": "Antibody response of patients with malignancies to bacteremia with gram-negative bacteria.", "content": "Antibody response, determined by means of the indirect bacterial hemagglutination tests, was studied in 58 consecutive patients with various malignancies whose blood culture yielded growth of Enterobacteriaceae or Pseudomonas and from whom serum specimens were obtained. Of these patients 59% had a significant antibody response. The invading microorganisms were Escherichia coli in 33 and Klebsiella in 19 subjects, an antibody response being documented with essentially equal frequency (60 and 57% of the subjects, respectively). Two patients had positive blood cultures for both E. coli and Klebsiella, one of whom had a significant response to one isolate only. A specific antibody resonse was documented in 67% of the subjects from whom blood for antibody titration was obtained at least 5 days after the blood culture, but from only 21% of patients whose serum was procured during the first 5 days after the blood culture. Similarly, such an antibody response was identified in 73% of subjects with two consecutive serum specimens, but in only 28% of the patients with a single serum specimen for antibody titration. Documentation of the immune response may be of diagnostic aid in differentiating between infection and contamination even in patients with underlying malignancy and under potentially immunosuppressive therapy.", "contents": "Antibody response of patients with malignancies to bacteremia with gram-negative bacteria. Antibody response, determined by means of the indirect bacterial hemagglutination tests, was studied in 58 consecutive patients with various malignancies whose blood culture yielded growth of Enterobacteriaceae or Pseudomonas and from whom serum specimens were obtained. Of these patients 59% had a significant antibody response. The invading microorganisms were Escherichia coli in 33 and Klebsiella in 19 subjects, an antibody response being documented with essentially equal frequency (60 and 57% of the subjects, respectively). Two patients had positive blood cultures for both E. coli and Klebsiella, one of whom had a significant response to one isolate only. A specific antibody resonse was documented in 67% of the subjects from whom blood for antibody titration was obtained at least 5 days after the blood culture, but from only 21% of patients whose serum was procured during the first 5 days after the blood culture. Similarly, such an antibody response was identified in 73% of subjects with two consecutive serum specimens, but in only 28% of the patients with a single serum specimen for antibody titration. Documentation of the immune response may be of diagnostic aid in differentiating between infection and contamination even in patients with underlying malignancy and under potentially immunosuppressive therapy."}
{"id": "PMID:1100660", "title": "Laboratory experience with a radiometric method for detecting bacteremia.", "content": "Two bacteriologic systems for detecting bacteria in blood were compared; the automated radiometric BACTEC and the conventional method used in our laboratory for many years. BACTEC consisted of two bottles with 30 ml and the conventional method with 50 ml of media for aerobes and anaerobes. The BACTEC bottles were inoculated with 2 to 3 ml and the conventional with 4 to 5 ml of blood at the patient's bedside. Out of the 3,045 blood specimens cultured (804 patients), 262 (117 patients) were positive by one or both methods. The conventional system detected 5more cultures. The explanation of the differences is discussed. Positive blood cultures were detected by the BACTEC procedure as early as 6 h after the blood collection. In the first 24 h, on the average, 77% of aerobic organisms were detected by the BACTEC as compared to 48% by the conventional system. All anaerobic BACTEC cultures were positive within 4 days, whereas the conventional system detected at that time 74%. At day 4, 67% of fungi were detected by the BACTEC and only 27% by the conventional system. Of the 3,045 blood cultures examined by the BACTEC, 208 were recorded as false positive with growth index readings ranging from 30 to 59.", "contents": "Laboratory experience with a radiometric method for detecting bacteremia. Two bacteriologic systems for detecting bacteria in blood were compared; the automated radiometric BACTEC and the conventional method used in our laboratory for many years. BACTEC consisted of two bottles with 30 ml and the conventional method with 50 ml of media for aerobes and anaerobes. The BACTEC bottles were inoculated with 2 to 3 ml and the conventional with 4 to 5 ml of blood at the patient's bedside. Out of the 3,045 blood specimens cultured (804 patients), 262 (117 patients) were positive by one or both methods. The conventional system detected 5more cultures. The explanation of the differences is discussed. Positive blood cultures were detected by the BACTEC procedure as early as 6 h after the blood collection. In the first 24 h, on the average, 77% of aerobic organisms were detected by the BACTEC as compared to 48% by the conventional system. All anaerobic BACTEC cultures were positive within 4 days, whereas the conventional system detected at that time 74%. At day 4, 67% of fungi were detected by the BACTEC and only 27% by the conventional system. Of the 3,045 blood cultures examined by the BACTEC, 208 were recorded as false positive with growth index readings ranging from 30 to 59."}
{"id": "PMID:1100661", "title": "Assessment of virus infectivity by the immunofluorescent and immunoperoxidase techniques.", "content": "The immunofluorescent and immunoperoxidase cell-counting techniques were comparable in precision and reproducibility for the quantitative assessment of influenza virus infectivity. The dose-response function was linear with each procedure, and comparable results were obtained for estimating neutralizing antibodies in antiviral serum.", "contents": "Assessment of virus infectivity by the immunofluorescent and immunoperoxidase techniques. The immunofluorescent and immunoperoxidase cell-counting techniques were comparable in precision and reproducibility for the quantitative assessment of influenza virus infectivity. The dose-response function was linear with each procedure, and comparable results were obtained for estimating neutralizing antibodies in antiviral serum."}
{"id": "PMID:1100662", "title": "A simple test system for the separation of staphylococci from micrococci.", "content": "A simple test system for the separation of staphylococci from micrococci is described, which is based on the ability of staphylococci to produce acid aerobically from glycerol in the presence of 0.4 mug of erythromycin per ml and on their sensitivity to lysostaphin.", "contents": "A simple test system for the separation of staphylococci from micrococci. A simple test system for the separation of staphylococci from micrococci is described, which is based on the ability of staphylococci to produce acid aerobically from glycerol in the presence of 0.4 mug of erythromycin per ml and on their sensitivity to lysostaphin."}
{"id": "PMID:1100663", "title": "Fluorescent antibody studies in chlamydial infections.", "content": "Irradiated McCoy cells infected with genital strains of Chlamydia trachomatis were grown in wells on slides coated with polytetrafluoroethylene. The inclusions produced in this system formed the antigen in an indirect immunofluorescence test, which detected group-specific chlamydial antibodies in sera from patients attending veneral disease clinics. Chlamydial antibodies were found more frequently and in higher titer in sera from women attending veneral disease clinics then in sera from a less promiscuous population attending a Family Planning Association clinic. Paired sera from 13 patients with nongonococcal urethritis from whom chylamydiae had been isolated were tested against the homologous isolates; seroconversion was demonstrated in only one instance, and antibody was present in the first serum specimens of all the other patients. Chlamydia-specific immunoglobulin M was found in four of eight patients with psittacosis and in a proportion of sera from patients attending veneral disease and Family Planning Association clinics. The antigen for this immunofluorescence test can easily be prepared in laboratories with cell culture facilities for the isolation of C. trachomatis, and the test should be useful for laboratories which cannot undertake the micro-immunofluorescence test.", "contents": "Fluorescent antibody studies in chlamydial infections. Irradiated McCoy cells infected with genital strains of Chlamydia trachomatis were grown in wells on slides coated with polytetrafluoroethylene. The inclusions produced in this system formed the antigen in an indirect immunofluorescence test, which detected group-specific chlamydial antibodies in sera from patients attending veneral disease clinics. Chlamydial antibodies were found more frequently and in higher titer in sera from women attending veneral disease clinics then in sera from a less promiscuous population attending a Family Planning Association clinic. Paired sera from 13 patients with nongonococcal urethritis from whom chylamydiae had been isolated were tested against the homologous isolates; seroconversion was demonstrated in only one instance, and antibody was present in the first serum specimens of all the other patients. Chlamydia-specific immunoglobulin M was found in four of eight patients with psittacosis and in a proportion of sera from patients attending veneral disease and Family Planning Association clinics. The antigen for this immunofluorescence test can easily be prepared in laboratories with cell culture facilities for the isolation of C. trachomatis, and the test should be useful for laboratories which cannot undertake the micro-immunofluorescence test."}
{"id": "PMID:1100664", "title": "A more complete evaluation of the color-coded antigen for the automated reagin test.", "content": "A comparative study, on 500 samples, of the toluidine red antigen, one of the low-cost, color-coded antigens for the Automated Reagin Test, shows a greater sensitivity than the rapid plasma reagin card antigen.", "contents": "A more complete evaluation of the color-coded antigen for the automated reagin test. A comparative study, on 500 samples, of the toluidine red antigen, one of the low-cost, color-coded antigens for the Automated Reagin Test, shows a greater sensitivity than the rapid plasma reagin card antigen."}
{"id": "PMID:1100665", "title": "Recovery of Cryptococcus neoformans from modified Dubos liquid medium utilized for isolation of mycobacteria.", "content": "Cryptococcus neoformans was isolated from nine pathological specimens cultured for mycobacteria. Five of these were recovered only in liquid TBC medium in the absence of growth on conventional substrates.", "contents": "Recovery of Cryptococcus neoformans from modified Dubos liquid medium utilized for isolation of mycobacteria. Cryptococcus neoformans was isolated from nine pathological specimens cultured for mycobacteria. Five of these were recovered only in liquid TBC medium in the absence of growth on conventional substrates."}
{"id": "PMID:1100666", "title": "Serological diagnosis of California (La Crosse) encephalitis by immunofluorescence.", "content": "A method of indirect immunofluorescence was developed and examined retrospectively as a serological test for the laboratory diagnosis of California encephalitis (CE). LaCrosse virus immunofluorescence immunoglobulin (Ig) G and IgM studies were done on paired sera from 50 patients with acute central nervous system infections. CE had been documented in 25 patients by hemagglutination inhibition, neutralizing, complement fixing, and/or precipitin tests. Five (20%) of the acute and 16 (64%) of the convalescent sera from CE patients had La Crosse IgM antibodies. Seven (28%) of the acute and all of the convalescent CE specimens had La Crosse IgG antibodies. Titers ranged from less than 4 to 256. IgG antibodies were present in all 11 sera collected 1 to 2 years after CE, but IgM antibodies were absent. The 25 serum pairs from patients who did not have CE were negative for IgM and IgG antibodies. This study indicated that La Crosse immunofluorescence antibody tests were as sensitive and specific for CE as conventional hemagglutination-inhibition tests, and would detect at least 20% of patients during their acute illness.", "contents": "Serological diagnosis of California (La Crosse) encephalitis by immunofluorescence. A method of indirect immunofluorescence was developed and examined retrospectively as a serological test for the laboratory diagnosis of California encephalitis (CE). LaCrosse virus immunofluorescence immunoglobulin (Ig) G and IgM studies were done on paired sera from 50 patients with acute central nervous system infections. CE had been documented in 25 patients by hemagglutination inhibition, neutralizing, complement fixing, and/or precipitin tests. Five (20%) of the acute and 16 (64%) of the convalescent sera from CE patients had La Crosse IgM antibodies. Seven (28%) of the acute and all of the convalescent CE specimens had La Crosse IgG antibodies. Titers ranged from less than 4 to 256. IgG antibodies were present in all 11 sera collected 1 to 2 years after CE, but IgM antibodies were absent. The 25 serum pairs from patients who did not have CE were negative for IgM and IgG antibodies. This study indicated that La Crosse immunofluorescence antibody tests were as sensitive and specific for CE as conventional hemagglutination-inhibition tests, and would detect at least 20% of patients during their acute illness."}
{"id": "PMID:1100667", "title": "Rapid identification of swarming Proteus using the PathoTec ornithine decarboxylase test strip.", "content": "The PathoTec ornithine decarboxylase test strip was evaluated for use in identification of swarming Proteus. All 181 strains tested were accurately identified within 4 h.", "contents": "Rapid identification of swarming Proteus using the PathoTec ornithine decarboxylase test strip. The PathoTec ornithine decarboxylase test strip was evaluated for use in identification of swarming Proteus. All 181 strains tested were accurately identified within 4 h."}
{"id": "PMID:1100668", "title": "Growth potential of cottonseed culture media for various clinically significant aerobic bacteria.", "content": "Enzymatic hydrolysates of various cottonseed flours were prepared with the proteolytic enzymes bromelain, HT-200, Pronase, and trypsin. The growth of various aerobic bacteria of clinical significance in these hydrolysates was compared to that obtained with a standard casein-soybean peptone culture medium, Trypticase soy. The generation times of the majority of bacteria grown in the bromelain cottonseed flour hydrolysate were shorter than that obtained with the standard control broth. A bromelain cottonseed flour hydrolysate agar preparation supported the growth of the bacteria comparably to that of the casein-soybean agar substrate. All the bacterial colonies were larger on the bromelain cottonseed flour hydrolysate blood agar medium than those grown on the control agar. The peptones derived from the enzymatic hydrolysis of cottonseed flour are sufficient to promote the rapid and luxuriant growth of a wide spectrum of aerobic bacteria without the addition of peptone from other sources. It is suggested that cottonseed flour peptones be utilized as a nutrient source in general-purpose media for the clinical microbiology laboratory.", "contents": "Growth potential of cottonseed culture media for various clinically significant aerobic bacteria. Enzymatic hydrolysates of various cottonseed flours were prepared with the proteolytic enzymes bromelain, HT-200, Pronase, and trypsin. The growth of various aerobic bacteria of clinical significance in these hydrolysates was compared to that obtained with a standard casein-soybean peptone culture medium, Trypticase soy. The generation times of the majority of bacteria grown in the bromelain cottonseed flour hydrolysate were shorter than that obtained with the standard control broth. A bromelain cottonseed flour hydrolysate agar preparation supported the growth of the bacteria comparably to that of the casein-soybean agar substrate. All the bacterial colonies were larger on the bromelain cottonseed flour hydrolysate blood agar medium than those grown on the control agar. The peptones derived from the enzymatic hydrolysis of cottonseed flour are sufficient to promote the rapid and luxuriant growth of a wide spectrum of aerobic bacteria without the addition of peptone from other sources. It is suggested that cottonseed flour peptones be utilized as a nutrient source in general-purpose media for the clinical microbiology laboratory."}
{"id": "PMID:1100669", "title": "Pigment production by Cryptococcus neoformans from para- and ortho-Diphenols: effect of the nitrogen source.", "content": "Cryptococcus neoformans produced pigments when p-diphenols were substrates in a glucose-amino acid-salts medium. The best substrates were 2.5-dihydroxybenzoic acid and 2,5-dihydroxybenzenesulfonic acid. In contrast to the cellular pigment production from o-diphenols (hydroxyl groups in the 2,3- or 3,4-position of phenyl ring), the p-diphenols (1,4- or 2,5-positions for the hydroxyl groups) produced large amounts of soluble pigments that diffused into the medium. When an optimal source of nitrogen (glutamine, glycine, and asparagine) was used, 89% of the C. neoformans strains produced pigments from p-diphenols. In contrast, 0 to 67% of the strains produced pigments when a suboptimal nitrogen source (proline, ammonium sulfate, ornithine, and methionine) was used. When glutamine-glycine-asparagine was the nitrogen source, 100% of the C. neoformans strains produced pigments from o0diphenols, whereas 77 to 100% of the strains produced pigment when proline-ammonium sulfate-ornithine-methionine was the nitrogen source. Cryptococcus species other than C. neoformans and all tested Candida species failed to produce pigments from any of the substrates except when hydroquinone was used. A combination of glutamine-glycine-asparagine and 3,4-dihydroxyphenylalanine allowed differentiation of colonies of C. neoformans from C. albicans in 3 to 6 days. These data showed that pigment production from o- and p-diphenols served as an excellent biochemical test for the identification of C. neoformans.", "contents": "Pigment production by Cryptococcus neoformans from para- and ortho-Diphenols: effect of the nitrogen source. Cryptococcus neoformans produced pigments when p-diphenols were substrates in a glucose-amino acid-salts medium. The best substrates were 2.5-dihydroxybenzoic acid and 2,5-dihydroxybenzenesulfonic acid. In contrast to the cellular pigment production from o-diphenols (hydroxyl groups in the 2,3- or 3,4-position of phenyl ring), the p-diphenols (1,4- or 2,5-positions for the hydroxyl groups) produced large amounts of soluble pigments that diffused into the medium. When an optimal source of nitrogen (glutamine, glycine, and asparagine) was used, 89% of the C. neoformans strains produced pigments from p-diphenols. In contrast, 0 to 67% of the strains produced pigments when a suboptimal nitrogen source (proline, ammonium sulfate, ornithine, and methionine) was used. When glutamine-glycine-asparagine was the nitrogen source, 100% of the C. neoformans strains produced pigments from o0diphenols, whereas 77 to 100% of the strains produced pigment when proline-ammonium sulfate-ornithine-methionine was the nitrogen source. Cryptococcus species other than C. neoformans and all tested Candida species failed to produce pigments from any of the substrates except when hydroquinone was used. A combination of glutamine-glycine-asparagine and 3,4-dihydroxyphenylalanine allowed differentiation of colonies of C. neoformans from C. albicans in 3 to 6 days. These data showed that pigment production from o- and p-diphenols served as an excellent biochemical test for the identification of C. neoformans."}
{"id": "PMID:1100670", "title": "Construction of an interpretive pattern directory for the API 10 S kit and analysis of its diagnostic accuracy.", "content": "A directory of test patterns and their interpretations has been prepared for identification of Enterobacteriaceae by using the 11-test API 10 S kit. The diagnostic accuracy of the directory and kit were evaluated by using records of test results for 37,476 isolates studied with the 21-test API 20 Enteric kit. Analysis indicates that 96.9% of the isolates would have been correctly identified at the genus level and 95.9% at the species level by using only the subset of tests included in the API 10 S.", "contents": "Construction of an interpretive pattern directory for the API 10 S kit and analysis of its diagnostic accuracy. A directory of test patterns and their interpretations has been prepared for identification of Enterobacteriaceae by using the 11-test API 10 S kit. The diagnostic accuracy of the directory and kit were evaluated by using records of test results for 37,476 isolates studied with the 21-test API 20 Enteric kit. Analysis indicates that 96.9% of the isolates would have been correctly identified at the genus level and 95.9% at the species level by using only the subset of tests included in the API 10 S."}
{"id": "PMID:1100671", "title": "Anaerobic bag culture method.", "content": "In a new method of anaerobic culture, a transparent, gas-impermeable bag is used and the anaerobic environment is established with copper sulfate-saturated steel wool. An Alka-Seltzer tablet generates carbon dioxide. The agar plate surface can be inspected through the bag at any time without interrupting the anaerobic atmosphere or disturbing other specimens. Methylene blue indicator strips are completely reduced by 4 h after the bag is set up and have remained reduced for as long as 3 weeks. Growth of 16 different stock culture anaerobes was generally equivalent by the bag and GasPak jar methods. Yield and growth of anaerobic isolates also were equivalent with 7 of 10 clinical specimens; from the other 3 specimens, 13 isolates were recovered, 5 by both the bag and jar methods and the rest by one method or the other. No consistent differences were found between the anaerobic bag and GasPak jar methods in the yield of anaerobes from clinical specimens. Early growth (24 h of incubation) of anaerobes from one specimen was detected with the bag method.", "contents": "Anaerobic bag culture method. In a new method of anaerobic culture, a transparent, gas-impermeable bag is used and the anaerobic environment is established with copper sulfate-saturated steel wool. An Alka-Seltzer tablet generates carbon dioxide. The agar plate surface can be inspected through the bag at any time without interrupting the anaerobic atmosphere or disturbing other specimens. Methylene blue indicator strips are completely reduced by 4 h after the bag is set up and have remained reduced for as long as 3 weeks. Growth of 16 different stock culture anaerobes was generally equivalent by the bag and GasPak jar methods. Yield and growth of anaerobic isolates also were equivalent with 7 of 10 clinical specimens; from the other 3 specimens, 13 isolates were recovered, 5 by both the bag and jar methods and the rest by one method or the other. No consistent differences were found between the anaerobic bag and GasPak jar methods in the yield of anaerobes from clinical specimens. Early growth (24 h of incubation) of anaerobes from one specimen was detected with the bag method."}
{"id": "PMID:1100672", "title": "Analysis of acetoin and diacetyl in bacterial culture supernatants by gas-liquid chromatography.", "content": "The acetoin and diacetyl contents of culture supernatants of Voges-Proskauer-positive \"viridans\" streptotocci, Klebsiella pneumoniae and Staphylococcus aureus, were determined by a gas liquid chromatographic procedure, in which supernatants were extracted with diethyl ether and diacetyl was measured on columns of 10% (wt/wt) polyethylene glycol 400 (PEG 400) at 73 C. Acetoin was converted to diacetyl, before analysis, by a simple oxidation procedure with ferric chloride and without a distillation step. Streptococcal culture supernatants were shown by this method to contain only acetoin; supernatants of K. pneumoniae and S. aureus contained both acetoin and diacetyl.", "contents": "Analysis of acetoin and diacetyl in bacterial culture supernatants by gas-liquid chromatography. The acetoin and diacetyl contents of culture supernatants of Voges-Proskauer-positive \"viridans\" streptotocci, Klebsiella pneumoniae and Staphylococcus aureus, were determined by a gas liquid chromatographic procedure, in which supernatants were extracted with diethyl ether and diacetyl was measured on columns of 10% (wt/wt) polyethylene glycol 400 (PEG 400) at 73 C. Acetoin was converted to diacetyl, before analysis, by a simple oxidation procedure with ferric chloride and without a distillation step. Streptococcal culture supernatants were shown by this method to contain only acetoin; supernatants of K. pneumoniae and S. aureus contained both acetoin and diacetyl."}
{"id": "PMID:1100673", "title": "Comparison of three tests for the serological diagnosis of lymphocytic choriomeningitis virus infection.", "content": "Levels of lymphocytic choriomeningitis virus antibody were assayed in 62 infected persons. The three tests used were indirect fluorescent antibody (IFA), complement fixation, and neutralization in mice. The sera first became positive by the IFA test, and IFA titers rapidly rose to a relatively high level, with the sera remaining positive long after the antibody detectable by complement fixation had disappeared. The IFA test appeared to be specific. The sera became positive last by the mouse neutralization test; with this test, antibody first appeared several weeks after infection. Virus-infected cells were stable when stored at -60 C, allowing diagnostic sera to be tested promptly by the IFA test. The IFA test for lymphocytic choriomeningitis antibody should increase the number of serological diagnoses, since it is not only rapid and specific, but detects cases not diagnosed by the other methods.", "contents": "Comparison of three tests for the serological diagnosis of lymphocytic choriomeningitis virus infection. Levels of lymphocytic choriomeningitis virus antibody were assayed in 62 infected persons. The three tests used were indirect fluorescent antibody (IFA), complement fixation, and neutralization in mice. The sera first became positive by the IFA test, and IFA titers rapidly rose to a relatively high level, with the sera remaining positive long after the antibody detectable by complement fixation had disappeared. The IFA test appeared to be specific. The sera became positive last by the mouse neutralization test; with this test, antibody first appeared several weeks after infection. Virus-infected cells were stable when stored at -60 C, allowing diagnostic sera to be tested promptly by the IFA test. The IFA test for lymphocytic choriomeningitis antibody should increase the number of serological diagnoses, since it is not only rapid and specific, but detects cases not diagnosed by the other methods."}
{"id": "PMID:1100674", "title": "Evaluation of antibody coating of yeasts in urine as an indicator of the site of urinary tract infection.", "content": "Antibody coating of yeasts (Candida sp. and Torulopsis sp.) found in urine specimens was investigated to ascertain whether the presence of such coating might identify the site of urinary tract infection. Washed yeast cells obtained by centrifugation of fresh urine specimens were reacted with fluorescein-conjugated goat antihuman immunoglobulins (Ig) G, A, and M and examined by fluorescent microscopy. IgG was found on the surface of all species of yeast encountered in all urine specimens evaluted, whereas there was variability of IgA AND IgM coating. Antibody coating with IgG, IgA, AND IgM was also demonstrated on yeasts from other body sites (sputum, gastrostomy, oral, etc.). Control experiments confirmed the specificity of the reactions. Thus, it appears that yeasts from any body site are coated with antibodies. These results are in contrast to recent work with bacteria which showed that the presence of antibody-coated (IgG) bacteria indicates upper urinary tract infection (pyelonephritis) while bacteria are not coated with antibodies in lower urinary tract infection. Since all yeasts from all body sites tested were found to be coated with antibody regardless of the clinical situation, the presence of surface antibody has no diagnostic value in identifying the site of urinary tract infection with yeasts.", "contents": "Evaluation of antibody coating of yeasts in urine as an indicator of the site of urinary tract infection. Antibody coating of yeasts (Candida sp. and Torulopsis sp.) found in urine specimens was investigated to ascertain whether the presence of such coating might identify the site of urinary tract infection. Washed yeast cells obtained by centrifugation of fresh urine specimens were reacted with fluorescein-conjugated goat antihuman immunoglobulins (Ig) G, A, and M and examined by fluorescent microscopy. IgG was found on the surface of all species of yeast encountered in all urine specimens evaluted, whereas there was variability of IgA AND IgM coating. Antibody coating with IgG, IgA, AND IgM was also demonstrated on yeasts from other body sites (sputum, gastrostomy, oral, etc.). Control experiments confirmed the specificity of the reactions. Thus, it appears that yeasts from any body site are coated with antibodies. These results are in contrast to recent work with bacteria which showed that the presence of antibody-coated (IgG) bacteria indicates upper urinary tract infection (pyelonephritis) while bacteria are not coated with antibodies in lower urinary tract infection. Since all yeasts from all body sites tested were found to be coated with antibody regardless of the clinical situation, the presence of surface antibody has no diagnostic value in identifying the site of urinary tract infection with yeasts."}
{"id": "PMID:1100675", "title": "Detection of fungi in clinical specimens by phase-contrast microscopy.", "content": "During 1973 and 1974, the following fungi were detected in clinical specimens by using phase-contrast microscopy: Blastomyces dermatitidis, 5; Coccidioides immitis, 3; Cryptococcus neoformans, 11; other yeasts 918; dermatophytes, 863; Mucor species, 1; and Aspergillus fumigatus, 16. This technique allows rapid detection and, in many instances, immediate identification of fungi in clinical specimens.", "contents": "Detection of fungi in clinical specimens by phase-contrast microscopy. During 1973 and 1974, the following fungi were detected in clinical specimens by using phase-contrast microscopy: Blastomyces dermatitidis, 5; Coccidioides immitis, 3; Cryptococcus neoformans, 11; other yeasts 918; dermatophytes, 863; Mucor species, 1; and Aspergillus fumigatus, 16. This technique allows rapid detection and, in many instances, immediate identification of fungi in clinical specimens."}
{"id": "PMID:1100677", "title": "The classification of depressive disorders. II. A review of historical and physiological classification studies.", "content": "A review of recent classification studies of depressive disorders based on historical data and physiological indices was made. It was found that historical variables, such as age of onset and family background, are potentially useful to distinguish depressive subgroups, although these groupings did not differentiate consistently unipolar from bipolar depressives. While the physiological indices do not support the present psychiatric nosology, single physical signs were found to differentiate subgroups within the effective disorders. The implications of these findings for a different research model were discussed.", "contents": "The classification of depressive disorders. II. A review of historical and physiological classification studies. A review of recent classification studies of depressive disorders based on historical data and physiological indices was made. It was found that historical variables, such as age of onset and family background, are potentially useful to distinguish depressive subgroups, although these groupings did not differentiate consistently unipolar from bipolar depressives. While the physiological indices do not support the present psychiatric nosology, single physical signs were found to differentiate subgroups within the effective disorders. The implications of these findings for a different research model were discussed."}
{"id": "PMID:1100678", "title": "A comparison of muscle relaxation training and music in the reduction of state and trait anxiety.", "content": "A comparison was made between muscle-relaxation training and relaxing music in the reduction of state and trait anxiety. Ss were 108 anxious female college students, half of whom received three sessions in muscle-relaxation training and half of whom received three sessions of relaxing music. Results indicate that both types of treatment significantly reduce state anxiety, but neither reduces trait anxiety. Results support Spielberger's state-trait anxiety theory and hold some implication for treatment.", "contents": "A comparison of muscle relaxation training and music in the reduction of state and trait anxiety. A comparison was made between muscle-relaxation training and relaxing music in the reduction of state and trait anxiety. Ss were 108 anxious female college students, half of whom received three sessions in muscle-relaxation training and half of whom received three sessions of relaxing music. Results indicate that both types of treatment significantly reduce state anxiety, but neither reduces trait anxiety. Results support Spielberger's state-trait anxiety theory and hold some implication for treatment."}
{"id": "PMID:1100679", "title": "Immunofluorescent detection of alpha1-antitrypsin in paraffin embedded liver tissue.", "content": "Alpha1-antitrypsin was detected by indirect immunofluorescence in frozen sections of liver biopsies from patients with clinically and biochemically proven alpha1-antitrypsin deficiency. The antigen could also be demonstrated in those liver specimens of the same patients which were fixed in Bouin's fluid and embedded in paraffin. The cellular localization and the brightness of the fluorescence were the same in both frozen and paraffin sections. Four additional biopsies from three other patients were selected on the basis of PAS-positive diastase-resistant inclusions reported in the hepatocytes. All these biopsies showed bright fluorescence in the cytoplasm of the liver cells although one of the biopsies was stored for as long as eight years. Specific fluorescence was constantly found in the periportal hepatocytes with varying degrees of positivity. No fluorescence was observed in the six control biopsies from patients with various other liver diseases. These findings prove that paraffin embedded specimens are suitable for immunofluorescence detection of alpha1-antitrypsin and that a retrospective study on old paraffin blocks is possible.", "contents": "Immunofluorescent detection of alpha1-antitrypsin in paraffin embedded liver tissue. Alpha1-antitrypsin was detected by indirect immunofluorescence in frozen sections of liver biopsies from patients with clinically and biochemically proven alpha1-antitrypsin deficiency. The antigen could also be demonstrated in those liver specimens of the same patients which were fixed in Bouin's fluid and embedded in paraffin. The cellular localization and the brightness of the fluorescence were the same in both frozen and paraffin sections. Four additional biopsies from three other patients were selected on the basis of PAS-positive diastase-resistant inclusions reported in the hepatocytes. All these biopsies showed bright fluorescence in the cytoplasm of the liver cells although one of the biopsies was stored for as long as eight years. Specific fluorescence was constantly found in the periportal hepatocytes with varying degrees of positivity. No fluorescence was observed in the six control biopsies from patients with various other liver diseases. These findings prove that paraffin embedded specimens are suitable for immunofluorescence detection of alpha1-antitrypsin and that a retrospective study on old paraffin blocks is possible."}
{"id": "PMID:1100680", "title": "Variants of Escherichia coli giving the appearance of mixed growths in urine.", "content": "Urines from patients with symptoms of urinary tract infection yielded mixed growths of different colony types of Escherichia coli. The different colony types were found to be variants of single infecting strains caused by mutation or by phase action. It is suggested that care should be exercised in the interpretation of apparently mixed growths from urine.", "contents": "Variants of Escherichia coli giving the appearance of mixed growths in urine. Urines from patients with symptoms of urinary tract infection yielded mixed growths of different colony types of Escherichia coli. The different colony types were found to be variants of single infecting strains caused by mutation or by phase action. It is suggested that care should be exercised in the interpretation of apparently mixed growths from urine."}
{"id": "PMID:1100681", "title": "Microbial flora of the lower genital tract during pregnancy: relationship to morbidity.", "content": "Nineteen genera and groups of micro-organisms were isolated from the lower genital tract of 280 women at their first antenatal visit. Chlamydia, viruses, and T-strain mycoplasmas were not sought, and only routine methods of anaerobic culture were used. Growth was recorded as scanty, moderate or heavy. The population studied was grouped according to age, parity, gestational stage at booking, presence and degree of severity of lower genital tract morbidity, past history of vulvovaginitis, and suspicion of lower genital tract morbidity as evidenced by a request for a report on the microbiological findings. The frequency of isolation of the various microbes in health and in disease is given. The grading of Gram-stained smears bore no relation to the isolation rates of lactobacilli, but there was a significant increase (p less than 0-001) in the isolation rates of each of the following: Mycoplasma hominis, Bacteroides spp., Trichomonas vaginalis, Gram-variable cocco-bacilli, and anaerobic streptococci in those patients with smears in which lactobacilli were adjudged to be absent. The isolation of faecal streptococci was increased (p less than 0-001) in women aged more than 34 years. Escherichia coli (p less than 0-05) and anaerobic and microaerophilic streptococci (p less than 0-02) were isolated more frequently from those booking after the 25th week of pregnancy. The incidence of M. hominis (p less than 0-02) and of anaerobic streptococci (p less than 0-05) increased between the first and third trimesters. No significance positive correlations were established between the isolation rates of the various microbes and objective assessment of lower genital tract morbidity or the demonstration of pus cells, but lactobacilli were isolated less frequently (p less than 0-01) from those with morbidity. The isolation of Candida albicans (p less than 0-02), T. vaginalis (p less than 0-05), and M. hominis (p less than 0.05) was increased in patients in whom vulvovaginitis was suspected, and that of T. vaginalis (p less than 0-05) was increased in those with a past history of vulvovaginitis. The study indicates that, other than the pathogens T. vaginalis and C. albicans, only M. Hominis could be suspected, on statistical grounds, of being associated with disease of the lower genital tract during early pregnancy.", "contents": "Microbial flora of the lower genital tract during pregnancy: relationship to morbidity. Nineteen genera and groups of micro-organisms were isolated from the lower genital tract of 280 women at their first antenatal visit. Chlamydia, viruses, and T-strain mycoplasmas were not sought, and only routine methods of anaerobic culture were used. Growth was recorded as scanty, moderate or heavy. The population studied was grouped according to age, parity, gestational stage at booking, presence and degree of severity of lower genital tract morbidity, past history of vulvovaginitis, and suspicion of lower genital tract morbidity as evidenced by a request for a report on the microbiological findings. The frequency of isolation of the various microbes in health and in disease is given. The grading of Gram-stained smears bore no relation to the isolation rates of lactobacilli, but there was a significant increase (p less than 0-001) in the isolation rates of each of the following: Mycoplasma hominis, Bacteroides spp., Trichomonas vaginalis, Gram-variable cocco-bacilli, and anaerobic streptococci in those patients with smears in which lactobacilli were adjudged to be absent. The isolation of faecal streptococci was increased (p less than 0-001) in women aged more than 34 years. Escherichia coli (p less than 0-05) and anaerobic and microaerophilic streptococci (p less than 0-02) were isolated more frequently from those booking after the 25th week of pregnancy. The incidence of M. hominis (p less than 0-02) and of anaerobic streptococci (p less than 0-05) increased between the first and third trimesters. No significance positive correlations were established between the isolation rates of the various microbes and objective assessment of lower genital tract morbidity or the demonstration of pus cells, but lactobacilli were isolated less frequently (p less than 0-01) from those with morbidity. The isolation of Candida albicans (p less than 0-02), T. vaginalis (p less than 0-05), and M. hominis (p less than 0.05) was increased in patients in whom vulvovaginitis was suspected, and that of T. vaginalis (p less than 0-05) was increased in those with a past history of vulvovaginitis. The study indicates that, other than the pathogens T. vaginalis and C. albicans, only M. Hominis could be suspected, on statistical grounds, of being associated with disease of the lower genital tract during early pregnancy."}
{"id": "PMID:1100682", "title": "Evaluation of a dehydrated test strip for the detection of yeasts.", "content": "Use of a dehydrated test strip for the detection of yeasts is compared with traditional culture on Sabouraud's agar containing 50 mug/ml chloramphenicol. While the selective medium of the strip is satisfactory for the isolation of species of Candida, Torulopsis glabrata grows only very slowly. The strip has the advantage of a long storage life without deterioration, but a high cost may preclude general usage. The numbers of yeasts collected by a bacteriological swab disadvantages of the selective medium, and the value of direct microscopy in the examination of vaginal swabs are discussed.", "contents": "Evaluation of a dehydrated test strip for the detection of yeasts. Use of a dehydrated test strip for the detection of yeasts is compared with traditional culture on Sabouraud's agar containing 50 mug/ml chloramphenicol. While the selective medium of the strip is satisfactory for the isolation of species of Candida, Torulopsis glabrata grows only very slowly. The strip has the advantage of a long storage life without deterioration, but a high cost may preclude general usage. The numbers of yeasts collected by a bacteriological swab disadvantages of the selective medium, and the value of direct microscopy in the examination of vaginal swabs are discussed."}
{"id": "PMID:1100683", "title": "The effect of a chlorhexidine-containing gel on oral cleanliness and gingival health in young adults.", "content": "Students at a vocational school participated in a double-blind, cross-over study to test the effect of brushing with a 1% chlorhexidine-containing gel once a day. In the control period, a placebo gel was used. Each treatment period lasted for 4 weeks. This was subsequently followed by 4 weeks of ordinary toothbrushing. Any toothbrushing additional to the therapeutic brushing was performed with a standard commercial dentifrice without abrasives. In addition to the treatment groups, containing a total of 24 subjects, another group of 12 subjects received standardized oral hygiene instruction. Oral hygiene and gingival condition were assessed in all groups throughout the study. The chlorhexidine-containing gel seemed to have no effect on gingivitis and only a slight inhibitory effect on plaque formation. Furthermore, an increase in the degree and frequency of staining of the tooth surfaces related to the use of active gel were observed.", "contents": "The effect of a chlorhexidine-containing gel on oral cleanliness and gingival health in young adults. Students at a vocational school participated in a double-blind, cross-over study to test the effect of brushing with a 1% chlorhexidine-containing gel once a day. In the control period, a placebo gel was used. Each treatment period lasted for 4 weeks. This was subsequently followed by 4 weeks of ordinary toothbrushing. Any toothbrushing additional to the therapeutic brushing was performed with a standard commercial dentifrice without abrasives. In addition to the treatment groups, containing a total of 24 subjects, another group of 12 subjects received standardized oral hygiene instruction. Oral hygiene and gingival condition were assessed in all groups throughout the study. The chlorhexidine-containing gel seemed to have no effect on gingivitis and only a slight inhibitory effect on plaque formation. Furthermore, an increase in the degree and frequency of staining of the tooth surfaces related to the use of active gel were observed."}
{"id": "PMID:1100684", "title": "A study of cochlear innervation patterns in cats and rats with the Golgi method and Nomarkski Optics.", "content": "Cochlear innervation patterns were studied in infant cats and rats with the rapid Golgi method. Examination of thick serial sections and surface preparations with the differential interference contrast microscope (Nomarski optics) allowed direct visualization of individually impregnated spiral ganglion cells, complete with their peripheral processes and endings in the organ of Corti. Individually impregnated efferent fibers could  be recognized as heavily varicose axons that project radially to endings beneath inner and outer hair cells after taking a tangential course in the intraganglionic spiral bundle. It was often possible to visualize unimpregnated hair cells in contact with the impregnated endings of both types of fibers. There are at least two types of spiral ganglion cells in the cochlea of the infant cat and rat. One type innervates only inner hair cells by means of radial fibers. These ganglion cells constitute the overwhelming majority of ganglion cells impregnated in our preparations, and each cell typically innervates two inner hair cells. Hence, these ganglion cells establish nearly \"point-to-point\" connections between the auditory nerve and the organ of Corti. The other type of ganglion cell innervates  outer hair cells by means of long spiral fibers; each cell typically innervates many outer hair cells through the numerous angular enlargements and short end branches of its spiral fiber. In addition, a few of these spiral fibers also send branches to inner hair cells by means of short collaterals; it remains to be seen if such fibers also occur in mature cochleas. Efferent fibers have been traced to inner and outer hair cell regions. The simplest pattern is formed by fine beaded axons with only a few branches ending mainly beneath inner hair cells. More complex patterns are formed by larger axons with many branches ending beneath inner or outer hair cells. Many efferent fibers send branches to both inner and outer hair cells. Electrophysiological studies so far have not demonstrated different populations of units that clearly correspond to the spiral and radial fibers. Therefore, the physilogical differences between inner and outer hair cell innervation remain undefined.", "contents": "A study of cochlear innervation patterns in cats and rats with the Golgi method and Nomarkski Optics. Cochlear innervation patterns were studied in infant cats and rats with the rapid Golgi method. Examination of thick serial sections and surface preparations with the differential interference contrast microscope (Nomarski optics) allowed direct visualization of individually impregnated spiral ganglion cells, complete with their peripheral processes and endings in the organ of Corti. Individually impregnated efferent fibers could  be recognized as heavily varicose axons that project radially to endings beneath inner and outer hair cells after taking a tangential course in the intraganglionic spiral bundle. It was often possible to visualize unimpregnated hair cells in contact with the impregnated endings of both types of fibers. There are at least two types of spiral ganglion cells in the cochlea of the infant cat and rat. One type innervates only inner hair cells by means of radial fibers. These ganglion cells constitute the overwhelming majority of ganglion cells impregnated in our preparations, and each cell typically innervates two inner hair cells. Hence, these ganglion cells establish nearly \"point-to-point\" connections between the auditory nerve and the organ of Corti. The other type of ganglion cell innervates  outer hair cells by means of long spiral fibers; each cell typically innervates many outer hair cells through the numerous angular enlargements and short end branches of its spiral fiber. In addition, a few of these spiral fibers also send branches to inner hair cells by means of short collaterals; it remains to be seen if such fibers also occur in mature cochleas. Efferent fibers have been traced to inner and outer hair cell regions. The simplest pattern is formed by fine beaded axons with only a few branches ending mainly beneath inner hair cells. More complex patterns are formed by larger axons with many branches ending beneath inner or outer hair cells. Many efferent fibers send branches to both inner and outer hair cells. Electrophysiological studies so far have not demonstrated different populations of units that clearly correspond to the spiral and radial fibers. Therefore, the physilogical differences between inner and outer hair cell innervation remain undefined."}
{"id": "PMID:1100685", "title": "The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine-beta-hydroxylase as a marker.", "content": "A sensitive immunofluorescence technique was used to describe systematically the distrubution of dopamine-beta-hydroxylase (DBH)-containing cell bodies, non-terminal fiber pathways, and terminal fields in the brain of the male albino rat. DBH is the enzyme that catalyzes the conversion of dopamine to noradrenaline, and as such is useful as an anatomical marker for noradrenaline and possibly adrenaline neurons. The enzyme is not present in dopamine- or indolamine-containing neurons. Ten micron frozen sections (1-in 20 series) were prepared in the frontal, sagittal, and horizontal planes from the olfactory bulb to the upper cervical segments of the spinal cord; adjacent sections in each plane were stained for DBH and for cells (toluidine blue=azure II). An atlas consisting of 40 projection drawings of selected frontal sections illustrates the results of the investigation. DBH perikarya are confined to three groups in the pons and medulla: the well defined locus coeruleus, a more diffuse but continuous subcoeruleus group that arches through the pons and ventral medulla, and a third dorsal medullary group centered in the dorsal motor nucleus of the vagus. A single principal adrenergic fiber system distributes a great many of the axons from these neuron groups to a majority of nuclear areas in the brain. In the pons and medulla two components of the fiber system may be distinguished. A medullary branch may be followed from the posterior aspect of the subcoeruleus group dorsally and then anteriorly through the lateral tegmental field and ventral aspect of the vestibular complex to a position subjacent to the locus coeruleus, where it is joined by a subcoeruleus branch consisting of a large number of fibers coursing among cells along the length of the subcoeruleus group, and by fibers arising from the locus coeruleus. Anterior to the locus coeruleus the principal adrenergic bundle courses as a single fiber tract immediately ventrolateral to the central gray in the mesencephalon and in the zona incerta and substantia innominata in the diencephalon. At the level of the septal area separate bundles reach the cortex dorsally over the genu of the corpus calosum via the medial septal-diagonal band nuclei and the lateral septum and ventrally between the olfactory tubercle and caudate-putamen. In the medulla and pons adrenergic fibers undoubtedly course in both directions. Anterior to the most rostral pontine cell bodies, however, all fibers presumably ascend. Along the course of the bundle distinct branches emerge to innervate circumscribed terminal fields. In addition, certain regions of the brain such as the reticular formation and pontine gray receive diffuse DBH innervation derived from less clearly defined pathways. A small number of areas in the brain contain little or no detectable DBH. These include the caudate-putamen, nucleus accumbens, globus pallidus, olfactory tubercle, subthalamic nucleus, substantia nigra, pretectal area, third, fourth and sixth cranial verve nuclei, and the trapezoid body nucleus.", "contents": "The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine-beta-hydroxylase as a marker. A sensitive immunofluorescence technique was used to describe systematically the distrubution of dopamine-beta-hydroxylase (DBH)-containing cell bodies, non-terminal fiber pathways, and terminal fields in the brain of the male albino rat. DBH is the enzyme that catalyzes the conversion of dopamine to noradrenaline, and as such is useful as an anatomical marker for noradrenaline and possibly adrenaline neurons. The enzyme is not present in dopamine- or indolamine-containing neurons. Ten micron frozen sections (1-in 20 series) were prepared in the frontal, sagittal, and horizontal planes from the olfactory bulb to the upper cervical segments of the spinal cord; adjacent sections in each plane were stained for DBH and for cells (toluidine blue=azure II). An atlas consisting of 40 projection drawings of selected frontal sections illustrates the results of the investigation. DBH perikarya are confined to three groups in the pons and medulla: the well defined locus coeruleus, a more diffuse but continuous subcoeruleus group that arches through the pons and ventral medulla, and a third dorsal medullary group centered in the dorsal motor nucleus of the vagus. A single principal adrenergic fiber system distributes a great many of the axons from these neuron groups to a majority of nuclear areas in the brain. In the pons and medulla two components of the fiber system may be distinguished. A medullary branch may be followed from the posterior aspect of the subcoeruleus group dorsally and then anteriorly through the lateral tegmental field and ventral aspect of the vestibular complex to a position subjacent to the locus coeruleus, where it is joined by a subcoeruleus branch consisting of a large number of fibers coursing among cells along the length of the subcoeruleus group, and by fibers arising from the locus coeruleus. Anterior to the locus coeruleus the principal adrenergic bundle courses as a single fiber tract immediately ventrolateral to the central gray in the mesencephalon and in the zona incerta and substantia innominata in the diencephalon. At the level of the septal area separate bundles reach the cortex dorsally over the genu of the corpus calosum via the medial septal-diagonal band nuclei and the lateral septum and ventrally between the olfactory tubercle and caudate-putamen. In the medulla and pons adrenergic fibers undoubtedly course in both directions. Anterior to the most rostral pontine cell bodies, however, all fibers presumably ascend. Along the course of the bundle distinct branches emerge to innervate circumscribed terminal fields. In addition, certain regions of the brain such as the reticular formation and pontine gray receive diffuse DBH innervation derived from less clearly defined pathways. A small number of areas in the brain contain little or no detectable DBH. These include the caudate-putamen, nucleus accumbens, globus pallidus, olfactory tubercle, subthalamic nucleus, substantia nigra, pretectal area, third, fourth and sixth cranial verve nuclei, and the trapezoid body nucleus."}
{"id": "PMID:1100692", "title": "In vitro evaluation of marginal leakage of corrosion-resistant amalgam alloy.", "content": "Restorations placed without coating the cavity walls with cavity varnish exhibited significant marginal leakage at all time intervals; however, there appears to be some reduction of leakage after six months. Restorations placed after coating the cavity walls with cavity varnish demonstrated no leakage at forty-eight hours and three months. Velvalloy and Dispersalloy restorations exhibited no leakage at six months, although there was a slight leakage at one year. Spheraloy restorations with cavity varnish demonstrated some leakage at six months and one year. The results of this study appear to correlate with previous studies using the same technique to determine marginal leakage. Apparently, the corrosion resistance of the dispersed phase alloy does not increase the marginal leakage, when compared with the conventional and spherical alloys.", "contents": "In vitro evaluation of marginal leakage of corrosion-resistant amalgam alloy. Restorations placed without coating the cavity walls with cavity varnish exhibited significant marginal leakage at all time intervals; however, there appears to be some reduction of leakage after six months. Restorations placed after coating the cavity walls with cavity varnish demonstrated no leakage at forty-eight hours and three months. Velvalloy and Dispersalloy restorations exhibited no leakage at six months, although there was a slight leakage at one year. Spheraloy restorations with cavity varnish demonstrated some leakage at six months and one year. The results of this study appear to correlate with previous studies using the same technique to determine marginal leakage. Apparently, the corrosion resistance of the dispersed phase alloy does not increase the marginal leakage, when compared with the conventional and spherical alloys."}
{"id": "PMID:1100708", "title": "Pontics in fixed prostheses--status report. Council on Dental Materials and Devices.", "content": "It is clear that the choice and fabrication of a pontic demands as much care as any aspect of restorative planning and execution. To apply a particular pontic design arbitrarily is to disregard the work to date which appears to emphasize the individuality of each pontic situation. Existing designs appear to take into account every conceivable situation. Presently available materials seem adequate to meet requirements. The role of bacterial plaque control in the maintenance of all pontic materials and designs must be recognized. What is required is a dentist cognizant of the reasons for using a particular pontic in a particular situation and a patient highly motivated and instructed as to his responsibility in caring for this component of his prothesis.", "contents": "Pontics in fixed prostheses--status report. Council on Dental Materials and Devices. It is clear that the choice and fabrication of a pontic demands as much care as any aspect of restorative planning and execution. To apply a particular pontic design arbitrarily is to disregard the work to date which appears to emphasize the individuality of each pontic situation. Existing designs appear to take into account every conceivable situation. Presently available materials seem adequate to meet requirements. The role of bacterial plaque control in the maintenance of all pontic materials and designs must be recognized. What is required is a dentist cognizant of the reasons for using a particular pontic in a particular situation and a patient highly motivated and instructed as to his responsibility in caring for this component of his prothesis."}
{"id": "PMID:1100710", "title": "The isolation of salmonellas from British pork sausages and sausage meat.", "content": "Between 1969 and 1974, 1467 packets (3309 samples) of pork sausages and sausage meat produced by two large and two medium sized manufacturers and several local butchers were examined for the presence of salmonellas. Of these, 435 packets (786 samples) were found to contain salmonellas, but there was a wide variation in the isolation rates according to the producer. The salmonella incidence in samples from several small and two medium sized producers was low (0-11%) while the results from the two large producers investigated showed a striking difference, the rate of salmonella contamination in the product of one was low (about 2%) and in that of the other consistently high (40-60%). A comparison of liquid enrichment media, incubation temperatures and selective agar media was also carried out to determine the most efficient combination for the isolation of salmonellas from minced meat products. The results showed that (a) incubation of enrichment cultures at 43 degrees C. yielded a consistently greater number of salmonella isolations that at 37 degrees C., regardless of plating medium, (b) tetrathionate broth A (Rolfe) was superior to selenite broth as en enrichment medium at both 37 and 43 degrees C. and (c) brilliant green agar gave better results than deoxycholate citrate sucrose agar and bismuth sulphite agar as a selective medium.", "contents": "The isolation of salmonellas from British pork sausages and sausage meat. Between 1969 and 1974, 1467 packets (3309 samples) of pork sausages and sausage meat produced by two large and two medium sized manufacturers and several local butchers were examined for the presence of salmonellas. Of these, 435 packets (786 samples) were found to contain salmonellas, but there was a wide variation in the isolation rates according to the producer. The salmonella incidence in samples from several small and two medium sized producers was low (0-11%) while the results from the two large producers investigated showed a striking difference, the rate of salmonella contamination in the product of one was low (about 2%) and in that of the other consistently high (40-60%). A comparison of liquid enrichment media, incubation temperatures and selective agar media was also carried out to determine the most efficient combination for the isolation of salmonellas from minced meat products. The results showed that (a) incubation of enrichment cultures at 43 degrees C. yielded a consistently greater number of salmonella isolations that at 37 degrees C., regardless of plating medium, (b) tetrathionate broth A (Rolfe) was superior to selenite broth as en enrichment medium at both 37 and 43 degrees C. and (c) brilliant green agar gave better results than deoxycholate citrate sucrose agar and bismuth sulphite agar as a selective medium."}
{"id": "PMID:1100711", "title": "Contribution to the study of live streptomycin-dependent Salmonella vaccines: the problem of reversion to a virulent form.", "content": "The recovery of virulence by means of reversion of a live streptomycin-dependent (Sm D) Salmonella typhimurium vaccine was studied in CD-1 Swiss mice. Initially, a one-step Sm D mutant was obtained from a virulent streptomycin-sensitive (Sm S) S. typhimurium strain. Afterwards, two pools of streptomycin-independent (Sm I) revertants were prepared from the Sm D strain. The virulence of the Sm D strain and of the Sm I revertants was tested intraperitoneally. In the virulence testing the original suspension of the Sm I revertants, as well as their 1st and 10th passages on plain medium, medium+50 mug. streptomycin/ml. and medium+1000 mug. streptomycin/ml. were used. The results show that the Sm D mutant was avirulent, its avirulence being due to an intrinsic, genetic quality. The Sm I revertants, compared to the original Sm S strain, also displayed a lack of virulence. However, afterwards, the Sm I revertants behaved quite differently, according to their subsequent passages. Indeed, there was an increase in virulence after passages on plain medium, whereas similar passages on medium containing the drug, the virulence not only failed to increase, but disappeared almost completely. Moreover, the passages on medium containing 1000 mug. streptomycin/ml. induced a return to the status of drug-dependence. The danger of recovery of virulence by means of revertants is evaluated.", "contents": "Contribution to the study of live streptomycin-dependent Salmonella vaccines: the problem of reversion to a virulent form. The recovery of virulence by means of reversion of a live streptomycin-dependent (Sm D) Salmonella typhimurium vaccine was studied in CD-1 Swiss mice. Initially, a one-step Sm D mutant was obtained from a virulent streptomycin-sensitive (Sm S) S. typhimurium strain. Afterwards, two pools of streptomycin-independent (Sm I) revertants were prepared from the Sm D strain. The virulence of the Sm D strain and of the Sm I revertants was tested intraperitoneally. In the virulence testing the original suspension of the Sm I revertants, as well as their 1st and 10th passages on plain medium, medium+50 mug. streptomycin/ml. and medium+1000 mug. streptomycin/ml. were used. The results show that the Sm D mutant was avirulent, its avirulence being due to an intrinsic, genetic quality. The Sm I revertants, compared to the original Sm S strain, also displayed a lack of virulence. However, afterwards, the Sm I revertants behaved quite differently, according to their subsequent passages. Indeed, there was an increase in virulence after passages on plain medium, whereas similar passages on medium containing the drug, the virulence not only failed to increase, but disappeared almost completely. Moreover, the passages on medium containing 1000 mug. streptomycin/ml. induced a return to the status of drug-dependence. The danger of recovery of virulence by means of revertants is evaluated."}
{"id": "PMID:1100712", "title": "Experimental model of oral antityphoid vaccination with live streptomycin-dependent Salmonella typhimurium in C57BL/6 mice.", "content": "The present experimental model offered the opportunity to study particular aspects concerning the avirulence stability of live streptomycin-dependent (Sm D) Salmonella vaccines, under conditions resembling human enteric fever. The results seem to indicate that, in practice, the risk of reversion to a virulent form during oral antityphoid vaccination with Sm D strains remains slight even after the interruption of concomitant streptomycin administration.", "contents": "Experimental model of oral antityphoid vaccination with live streptomycin-dependent Salmonella typhimurium in C57BL/6 mice. The present experimental model offered the opportunity to study particular aspects concerning the avirulence stability of live streptomycin-dependent (Sm D) Salmonella vaccines, under conditions resembling human enteric fever. The results seem to indicate that, in practice, the risk of reversion to a virulent form during oral antityphoid vaccination with Sm D strains remains slight even after the interruption of concomitant streptomycin administration."}
{"id": "PMID:1100713", "title": "Comparison of two methods for assessing the removal of total organisms and pathogens from the skin.", "content": "A standard hand-wash sampling technique was compared with a simple finger-streak sampling method in assessing the relative effectiveness of a number of alternative preparations used for disinfecting the surgeon's hands (alcoholic 0.5% chlorhexidine, alcoholic 0.1% tetrabrom-o-methyl phenol, a 4% chlorhexidine detergent solution, aqueous 0.5% chlorhexidine, 2% 'Irgasan' detergent solution and, as control, bar soap). There was a fairly good correlation between the results of assessment by the two methods after a single disinfection and after six disinfections, three on one day and three on the next. Significant differences were shown in 21 comparisons between treatments when the hand-wash sampling test was used, and 16 of these comparisons also showed a significant difference by the finger-streak test. Staphylococcus aureus was found in hand samplings from 5 out of 8 nurses in the Burns Unit of Birmingham Accident Hospital by the hand-wash sampling method and from 2 of the same 8 nurses by the finger-streak method; the numbers were small, and no Staph. aureus were isolated from the same hands after 1 min. wash in 70% ethyl alcohol. Similar sampling on 29 nurses in other wards showed Staph. aureus on 3 nurses (one in large numbers) by the hand-wash technique and on 1 nurse by the finger-streak test; in only 1 nurse whose hands showed Staph. aureus before disinfection was the organism found, by hand-wash sampling, after disinfection. Parallel sampling of nurses' hands after washing with soap and water and after disinfection with 95% ethanol showed larger numbers of Staph. aureus in a hospital for skin diseases than in a general hospital, and a lower incidence and somewhat lower density of Staph. aureus after ethanol treatment than after washing with soap and water; Gram-negative bacilli, on the other hand, were commoner on hands in the general than in the skin hospital, and present in much smaller numbers after disinfection with ethanol than after washing with soap and water. Antibiotic sensitivity tests showed the frequent recurrence on the hands of some nurses of multi-resistant Staph. aureus with resistance patterns similar to those found in infective lesions in some of the patients; different sensitivity patterns were usually found in staphylococci isolated from the nose. Even in wards where many patients were infected, carriage by nurses' hands of a particular strain of Staph. aureus did not seem to last for more than a few days.", "contents": "Comparison of two methods for assessing the removal of total organisms and pathogens from the skin. A standard hand-wash sampling technique was compared with a simple finger-streak sampling method in assessing the relative effectiveness of a number of alternative preparations used for disinfecting the surgeon's hands (alcoholic 0.5% chlorhexidine, alcoholic 0.1% tetrabrom-o-methyl phenol, a 4% chlorhexidine detergent solution, aqueous 0.5% chlorhexidine, 2% 'Irgasan' detergent solution and, as control, bar soap). There was a fairly good correlation between the results of assessment by the two methods after a single disinfection and after six disinfections, three on one day and three on the next. Significant differences were shown in 21 comparisons between treatments when the hand-wash sampling test was used, and 16 of these comparisons also showed a significant difference by the finger-streak test. Staphylococcus aureus was found in hand samplings from 5 out of 8 nurses in the Burns Unit of Birmingham Accident Hospital by the hand-wash sampling method and from 2 of the same 8 nurses by the finger-streak method; the numbers were small, and no Staph. aureus were isolated from the same hands after 1 min. wash in 70% ethyl alcohol. Similar sampling on 29 nurses in other wards showed Staph. aureus on 3 nurses (one in large numbers) by the hand-wash technique and on 1 nurse by the finger-streak test; in only 1 nurse whose hands showed Staph. aureus before disinfection was the organism found, by hand-wash sampling, after disinfection. Parallel sampling of nurses' hands after washing with soap and water and after disinfection with 95% ethanol showed larger numbers of Staph. aureus in a hospital for skin diseases than in a general hospital, and a lower incidence and somewhat lower density of Staph. aureus after ethanol treatment than after washing with soap and water; Gram-negative bacilli, on the other hand, were commoner on hands in the general than in the skin hospital, and present in much smaller numbers after disinfection with ethanol than after washing with soap and water. Antibiotic sensitivity tests showed the frequent recurrence on the hands of some nurses of multi-resistant Staph. aureus with resistance patterns similar to those found in infective lesions in some of the patients; different sensitivity patterns were usually found in staphylococci isolated from the nose. Even in wards where many patients were infected, carriage by nurses' hands of a particular strain of Staph. aureus did not seem to last for more than a few days."}
{"id": "PMID:1100714", "title": "The effect of antibiotic therapy on the faecal excretion of Salmonella typhimurium by experimentally infected chickens.", "content": "Chickens in groups of 40 were infected orally with a nalidixic acid-resistant mutant of Salmonella typhimurium and then fed continuously on diets containing ampicillin, chloramphenicol, furazolidone, neomycin, oxytetracycline, polymixin, spectinomycin, streptomycin or a mixture of trimethoprim and sulphadiazine. The amount of S. typhimurium excreted in their faeces was estimated at intervals by culture on brilliant green agar containing sodium nalidixate, both direct and after enrichment in selenite broth; the amount of Escherichia coli excreted was estimated by culture on MacConkey agar. The feeding of diets containing 500 mg./kg. of ampicillin, furazolidone, neomycin, polymixin, spectinomycin or streptomycin or 100 mg./kg. of trimethoprim and 500 mg./kg. of sulphadiazine for 46 days reduced to a varying degree the amount of S. typhimurium and E. coli excreted, the greatest reduction in S. typhimurium being brought about by the last treatment. The effect was less obvious when the concentration of the antibiotics in the food was decreased fivefold. An important reason for the very limited effect of some of the antibiotics was the emergence of antibiotic-resistant populations of S. typhimurium and E. coli. High concentrations of antibiotic-resistant organisms also arose in the faeces of the chickens fed diets containing tetracyclines and chloramphenicol, treatments which had no apparent effect on the amount of S. typhimurium and E. coli excreted. Much of the antibiotic resistance encountered was determined by R factors, a particular R factor usually being found in the E. coli populations of individual chickens before it was found in their S. typhimurium populations. No S. typhimurium or E. coli were isolated that possessed R factors determining resistance to polymixin, furazolidone or trimethoprim. No S. typhimurium or E. coli were isolated that were polymixin-resistant and no S. typhimurium that were furazolidone-resistant. The few trimethoprim-resistant S. typhimurium isolated were thymine-dependent. The feeding of diets containing the higher concentrations of trimethoprim/sulphadiazine, neomycin, furazolidone or ampicillin for 9 days reduced the amount of S. typhimurium excreted. After the withdrawal of these diets, the amount of S. typhimurium excreted increased to the numbers found in chickens given ordinary diets throughout; the chickens that had been given trimethoprim/sulphadiazine or furazolidone did not remain faecal excreters of S. typhimurium longer than the chickens that had been given ordinary diets. Similar results were obtained with trimethoprim/sulphadiazine when the start of the 9-day treatment period was delayed for an extra 9 days or when it was extended to 18 days.", "contents": "The effect of antibiotic therapy on the faecal excretion of Salmonella typhimurium by experimentally infected chickens. Chickens in groups of 40 were infected orally with a nalidixic acid-resistant mutant of Salmonella typhimurium and then fed continuously on diets containing ampicillin, chloramphenicol, furazolidone, neomycin, oxytetracycline, polymixin, spectinomycin, streptomycin or a mixture of trimethoprim and sulphadiazine. The amount of S. typhimurium excreted in their faeces was estimated at intervals by culture on brilliant green agar containing sodium nalidixate, both direct and after enrichment in selenite broth; the amount of Escherichia coli excreted was estimated by culture on MacConkey agar. The feeding of diets containing 500 mg./kg. of ampicillin, furazolidone, neomycin, polymixin, spectinomycin or streptomycin or 100 mg./kg. of trimethoprim and 500 mg./kg. of sulphadiazine for 46 days reduced to a varying degree the amount of S. typhimurium and E. coli excreted, the greatest reduction in S. typhimurium being brought about by the last treatment. The effect was less obvious when the concentration of the antibiotics in the food was decreased fivefold. An important reason for the very limited effect of some of the antibiotics was the emergence of antibiotic-resistant populations of S. typhimurium and E. coli. High concentrations of antibiotic-resistant organisms also arose in the faeces of the chickens fed diets containing tetracyclines and chloramphenicol, treatments which had no apparent effect on the amount of S. typhimurium and E. coli excreted. Much of the antibiotic resistance encountered was determined by R factors, a particular R factor usually being found in the E. coli populations of individual chickens before it was found in their S. typhimurium populations. No S. typhimurium or E. coli were isolated that possessed R factors determining resistance to polymixin, furazolidone or trimethoprim. No S. typhimurium or E. coli were isolated that were polymixin-resistant and no S. typhimurium that were furazolidone-resistant. The few trimethoprim-resistant S. typhimurium isolated were thymine-dependent. The feeding of diets containing the higher concentrations of trimethoprim/sulphadiazine, neomycin, furazolidone or ampicillin for 9 days reduced the amount of S. typhimurium excreted. After the withdrawal of these diets, the amount of S. typhimurium excreted increased to the numbers found in chickens given ordinary diets throughout; the chickens that had been given trimethoprim/sulphadiazine or furazolidone did not remain faecal excreters of S. typhimurium longer than the chickens that had been given ordinary diets. Similar results were obtained with trimethoprim/sulphadiazine when the start of the 9-day treatment period was delayed for an extra 9 days or when it was extended to 18 days."}
{"id": "PMID:1100715", "title": "The effect of feeding diets containing permitted antibiotics on the faecal excretion of Salmonella typhimurium by experimentally infected chickens.", "content": "Groups of 45 chickens were fed continuously on diets containing 10 or 100 mg./kg. of different 'growth-promoting' antibiotics and infected orally with a nalidixic acid-resistant mutant of Salmonella typhimurium. The amount of S. typhimurium organisms excreted in their faeces was estimated by culturing them at weekly intervals and in a standard manner on plates of brilliant green agar containing sodium nalidixate, both direct and after enrichment in selenite broth. All of four groups fed diets containing 100 mg./kg. of nitrovin in three different experiments excreted much larger amounts of S. typhimurium than did groups fed antibiotic-free diets. In some, but not all, experiments, larger amounts were also excreted by groups fed diets containing 10 mg./kg. of nitrovin or 10 or 100 mg./kg. of flavomycin or tylosin. Feeding diets containing 10 or 100 mg./kg. of virginiamycin or bacitracin either did not influence or slightly increased the amount of S. typhimiurium excreted. Two groups fed continuously on diets containing 100 or 500 mg./kg. of sulphaquinoxaline for 44 days excreted smaller amounts of the S. typhimurium organisms that did groups fed antibiotic-free diets; no sulphonamide-resistant organisms of the S. typhimurium strain were isolated from the faeces.", "contents": "The effect of feeding diets containing permitted antibiotics on the faecal excretion of Salmonella typhimurium by experimentally infected chickens. Groups of 45 chickens were fed continuously on diets containing 10 or 100 mg./kg. of different 'growth-promoting' antibiotics and infected orally with a nalidixic acid-resistant mutant of Salmonella typhimurium. The amount of S. typhimurium organisms excreted in their faeces was estimated by culturing them at weekly intervals and in a standard manner on plates of brilliant green agar containing sodium nalidixate, both direct and after enrichment in selenite broth. All of four groups fed diets containing 100 mg./kg. of nitrovin in three different experiments excreted much larger amounts of S. typhimurium than did groups fed antibiotic-free diets. In some, but not all, experiments, larger amounts were also excreted by groups fed diets containing 10 mg./kg. of nitrovin or 10 or 100 mg./kg. of flavomycin or tylosin. Feeding diets containing 10 or 100 mg./kg. of virginiamycin or bacitracin either did not influence or slightly increased the amount of S. typhimiurium excreted. Two groups fed continuously on diets containing 100 or 500 mg./kg. of sulphaquinoxaline for 44 days excreted smaller amounts of the S. typhimurium organisms that did groups fed antibiotic-free diets; no sulphonamide-resistant organisms of the S. typhimurium strain were isolated from the faeces."}
{"id": "PMID:1100716", "title": "Complex surveillance of Streptococcus pyogenes. II. Resistance in human blood and bactericidal activity receptors of field and collection streptococci.", "content": "A comparative study of a series of 31 epidemic streptococci and 36 collection M protein producers showed no statistically significant difference in their resistance in human blood. Even highly resistant field strains did not produce M protein (as the main bactericidal activity receptor) under laboratory conditions, however, although under natural conditions they left an anti-M response in the exposed population. Streptococcal resistance is not generally associated with M protein, but is influenced by another factor.", "contents": "Complex surveillance of Streptococcus pyogenes. II. Resistance in human blood and bactericidal activity receptors of field and collection streptococci. A comparative study of a series of 31 epidemic streptococci and 36 collection M protein producers showed no statistically significant difference in their resistance in human blood. Even highly resistant field strains did not produce M protein (as the main bactericidal activity receptor) under laboratory conditions, however, although under natural conditions they left an anti-M response in the exposed population. Streptococcal resistance is not generally associated with M protein, but is influenced by another factor."}
{"id": "PMID:1100717", "title": "Immunochemical properties of streptococcal M protein purified by isoelectric focusing.", "content": "Electrofucusing of an alkaline extract of type 24 streptococcal M protein yielded an antigenic fraction that was type specific and apparently homogeneous. The haptenic nature of this fraction was suggested by its inability to precipitate type-specific antiserum or to induce opsonic antibodies in rabbits, despite its ability to strongly inhibit opsonization of homologous-type streptococci by M antibody. The fraction migrated as a single band upon electrophoresis in sodium dodecyl sulfate (SDS) acrylamide gel. The mobility of the protein band was consistent with a molecular weight of 36,500 daltons. In some experiments using larger quantities of protein, a second faint protein band with an average molecular weight of 70,000 was observed, suggesting the presence of dimers of the 36,500-dalton protein. Amino acid analysis showed the predominant amino acid to be glycine followed by aspartic acid and glutamic acid. Moreover, this M protein fraction was free of non-type-specific immunotoxic properties in guinea pigs and in man. Although apparently not immunogenic, this nontoxic fraction may provide a useful tool to study the relationship of the type-specific protective moiety to potentially harmful \"impurities\" in M protein vaccines.", "contents": "Immunochemical properties of streptococcal M protein purified by isoelectric focusing. Electrofucusing of an alkaline extract of type 24 streptococcal M protein yielded an antigenic fraction that was type specific and apparently homogeneous. The haptenic nature of this fraction was suggested by its inability to precipitate type-specific antiserum or to induce opsonic antibodies in rabbits, despite its ability to strongly inhibit opsonization of homologous-type streptococci by M antibody. The fraction migrated as a single band upon electrophoresis in sodium dodecyl sulfate (SDS) acrylamide gel. The mobility of the protein band was consistent with a molecular weight of 36,500 daltons. In some experiments using larger quantities of protein, a second faint protein band with an average molecular weight of 70,000 was observed, suggesting the presence of dimers of the 36,500-dalton protein. Amino acid analysis showed the predominant amino acid to be glycine followed by aspartic acid and glutamic acid. Moreover, this M protein fraction was free of non-type-specific immunotoxic properties in guinea pigs and in man. Although apparently not immunogenic, this nontoxic fraction may provide a useful tool to study the relationship of the type-specific protective moiety to potentially harmful \"impurities\" in M protein vaccines."}
{"id": "PMID:1100718", "title": "Mouse effector functions involved in the antibody-dependent cellular cytotoxicity to xenogeneic erythrocytes.", "content": "Mouse spleen cell populations were fractionated by carbonyl iron depletion and nylon wool column separation. The fractions were tested for phagocytic and lytic activity against antibody-coated 51Cr-labeled sheep red blood cells. Carbonyl iron resistant cells had no phagocytic activity but caused lysis at a relatively slow rate. Nylon wool column nonadherent cells had a similar effect. In contrast with the slow lytic action of these cells, nylon wool adherent cells or non-carbonyl iron-treated cells had phagocytic as well as rapid lytic action. Under the conditions studied, both rapid and slow lytic functions were saturable whereas the phagocytic function was not.", "contents": "Mouse effector functions involved in the antibody-dependent cellular cytotoxicity to xenogeneic erythrocytes. Mouse spleen cell populations were fractionated by carbonyl iron depletion and nylon wool column separation. The fractions were tested for phagocytic and lytic activity against antibody-coated 51Cr-labeled sheep red blood cells. Carbonyl iron resistant cells had no phagocytic activity but caused lysis at a relatively slow rate. Nylon wool column nonadherent cells had a similar effect. In contrast with the slow lytic action of these cells, nylon wool adherent cells or non-carbonyl iron-treated cells had phagocytic as well as rapid lytic action. Under the conditions studied, both rapid and slow lytic functions were saturable whereas the phagocytic function was not."}
{"id": "PMID:1100719", "title": "Reactivity of antihuman thymocyte serum with acute leukemic blasts.", "content": "The presence of receptors for sheep erythrocytes (E) on some leukemic lymphoblasts is suggestive but not conclusive evidence for a thymic origin of these lymphoid cells. Normal and leukemic lymphoid populations were therefore examined to determine if the formation of spontaneous E rosettes correlated with the presence of thymus-associated cell surface antigens. Lymphoblasts from four of 12 children with acute lymphoblastic leukemia (ALL) formed spontaneous E rosettes at 4 degrees C and 37 degrees C (E+) and bound rabbit antihuman thymocyte serum, as determined by indirect immunofluorescence and by an indirect radiolabeled antibody assay. The blasts from the other eight children did not form E rosettes at 4 or 37 degrees C (E-) and did not bind antithymocyte serum. Absorption of the antiserum with peripheral blood leukocytes removed all detectable reactivity with normal peripheral blood lymphocytes, normal bone marrow cells, and E- bone marrow cells, but did not remove all reactivity with thymus cells or E+ leukemic blasts. Absorption of the antiserum with thymus from one donor removed all reactivity against thymus cells from another donor. After absorption with normal peripheral blood leukocytes, the antithymocyte serum reacted with E+ leukemic blasts but not with remission lymphocytes from the same patients. Thus, there are at least two distinct thymus-associated antigens-one that is present on T lymphocytes from peripheral blood as well as thymus and another that is present only on normal thymus and on ALL blasts that are identified by their ability to form E rosettes at 37 degrees C.", "contents": "Reactivity of antihuman thymocyte serum with acute leukemic blasts. The presence of receptors for sheep erythrocytes (E) on some leukemic lymphoblasts is suggestive but not conclusive evidence for a thymic origin of these lymphoid cells. Normal and leukemic lymphoid populations were therefore examined to determine if the formation of spontaneous E rosettes correlated with the presence of thymus-associated cell surface antigens. Lymphoblasts from four of 12 children with acute lymphoblastic leukemia (ALL) formed spontaneous E rosettes at 4 degrees C and 37 degrees C (E+) and bound rabbit antihuman thymocyte serum, as determined by indirect immunofluorescence and by an indirect radiolabeled antibody assay. The blasts from the other eight children did not form E rosettes at 4 or 37 degrees C (E-) and did not bind antithymocyte serum. Absorption of the antiserum with peripheral blood leukocytes removed all detectable reactivity with normal peripheral blood lymphocytes, normal bone marrow cells, and E- bone marrow cells, but did not remove all reactivity with thymus cells or E+ leukemic blasts. Absorption of the antiserum with thymus from one donor removed all reactivity against thymus cells from another donor. After absorption with normal peripheral blood leukocytes, the antithymocyte serum reacted with E+ leukemic blasts but not with remission lymphocytes from the same patients. Thus, there are at least two distinct thymus-associated antigens-one that is present on T lymphocytes from peripheral blood as well as thymus and another that is present only on normal thymus and on ALL blasts that are identified by their ability to form E rosettes at 37 degrees C."}
{"id": "PMID:1100720", "title": "Antibody-induced redistribution of CEA on the cell surface: utilization in separation of CEA and isoantigen A.", "content": "Antibodies specific for carcinoembryonic antigen (CEA) induced polar redistribution of CEA which was expressed on the surface of human intestinal carcinoma cells grown in tissue culture. Polar redistribution occurred at 23 degrees C and 37 degrees C but not at 4 degrees C and was inhibited by sodium azide and cytochalasin B. Antibodies to isoantigen A did not react with CEA at the cell surface but did react with isoantigen A which was not redistributed by the antibody. Cells which expressed both CEA and isoantigen A were used to demonstrate that polar redistribution of CEA could be induced without altering the distribution of isoantigen A. The data indicate that CEA and isoantigen A can be expressed on the same cell as separate molecules.", "contents": "Antibody-induced redistribution of CEA on the cell surface: utilization in separation of CEA and isoantigen A. Antibodies specific for carcinoembryonic antigen (CEA) induced polar redistribution of CEA which was expressed on the surface of human intestinal carcinoma cells grown in tissue culture. Polar redistribution occurred at 23 degrees C and 37 degrees C but not at 4 degrees C and was inhibited by sodium azide and cytochalasin B. Antibodies to isoantigen A did not react with CEA at the cell surface but did react with isoantigen A which was not redistributed by the antibody. Cells which expressed both CEA and isoantigen A were used to demonstrate that polar redistribution of CEA could be induced without altering the distribution of isoantigen A. The data indicate that CEA and isoantigen A can be expressed on the same cell as separate molecules."}
{"id": "PMID:1100721", "title": "Evidence for an abnormal microenvironment in the thymus of New Zealand black mice.", "content": "The proliferative response of NZB and DBA/2 thymocytes and spleen cells to allogeneic cells and mitogens was studied in intact and lethally irradiated bone marrow-repopulated syngeneic recipients. The major findings were complete recovery of function in NZB spleen cells and premature recovery in thymocytes compared to the control DBA/2 strain. This recovery was not influenced by the administration of thymosin to the recipients, and was not due to functional thymocytes present in the donor marrow inoculum. These results suggest normal or increased support of T cell differentiation by the radioresistant thymus in 4-month-old NZB mice. Thymic epithelial cell products regulating T cell maturation may be disordered or unbalanced but do not appear to be broadly deficient at this age.", "contents": "Evidence for an abnormal microenvironment in the thymus of New Zealand black mice. The proliferative response of NZB and DBA/2 thymocytes and spleen cells to allogeneic cells and mitogens was studied in intact and lethally irradiated bone marrow-repopulated syngeneic recipients. The major findings were complete recovery of function in NZB spleen cells and premature recovery in thymocytes compared to the control DBA/2 strain. This recovery was not influenced by the administration of thymosin to the recipients, and was not due to functional thymocytes present in the donor marrow inoculum. These results suggest normal or increased support of T cell differentiation by the radioresistant thymus in 4-month-old NZB mice. Thymic epithelial cell products regulating T cell maturation may be disordered or unbalanced but do not appear to be broadly deficient at this age."}
{"id": "PMID:1100722", "title": "Temporal dependence of the inhibition by cytochalasin B of antigen-initiated migration inhibition factor production.", "content": "The effects of cytochalasin B on the antigen recognition process have been examined using an in vitro assay of macrophage-inhibition factor (MIF) production by activated guinea pig T lymphocyte-enriched populations. The data indicate that cytochalasin B inhibits MIF production if added at the initiation of culture but not if added 2 hr after antigen. These data are interpreted as consistent with either an inhibition by cytochalasin B of the physical assoication of antigen-bearing marcrophage and lymphocytes or with an inhibition of the access of antigen to the T lymphocyte receptor.", "contents": "Temporal dependence of the inhibition by cytochalasin B of antigen-initiated migration inhibition factor production. The effects of cytochalasin B on the antigen recognition process have been examined using an in vitro assay of macrophage-inhibition factor (MIF) production by activated guinea pig T lymphocyte-enriched populations. The data indicate that cytochalasin B inhibits MIF production if added at the initiation of culture but not if added 2 hr after antigen. These data are interpreted as consistent with either an inhibition by cytochalasin B of the physical assoication of antigen-bearing marcrophage and lymphocytes or with an inhibition of the access of antigen to the T lymphocyte receptor."}
{"id": "PMID:1100723", "title": "A subpopulation of T cells bearing Fc receptors.", "content": "A wide range of cell populations were examined for Fc receptor (FcR)-bearing T cells: thymus, spleen, peritoneal cells, and T cells activated to H-2 antigens in spleen (ATC spleen) and in thoracic duct lymph (T-TDL). In addition, B lymphocytes from thoracic duct lymph of athymic nude mice and a Thy-1-positive, FcR-positive thymoma served as control cell populations. Reagents used were aggregates of human gamma-globulin and of various mouse myeloma proteins (IgG1, IgG2a, IgG2b), radioiodinated antigen-antibody complexes, and sheep erythrocyte antibody rosettes. Labeling techniques involving radioautography and immunofluorescence were used to demonstrate FcR by one of the above reagents and to identify T cells either by staining with anti-Thy-1.2 or by a specific rabbit anti-mouse T cell serum, or by failure to stain with anti-mouse immunoglobulin. In some experiments phagocytic cells were removed whereas in others they were identified by their capacity to engulf latex particles. Approximately 25% of cells with T cell markers were FcR-bearing cells in thymus, normal spleen, and peritoneal cavity, and 17% in ATC spleen. FcR on T cells in peripheral lymphoid tissues were detectable by aggregates of HGG and myeloma proteins and by radioiodinated immune complexes. Those on T cells in thymus were revealed only by aggregates of HGG. Circulating T cells (T.TDL) failed to display FcR: a) despite the use of a wide range of the above labeling techniques, each of which was shown to detect FcR on other T cells, thymoma cells, and B cells, and b) even after removal of Ig associated with their cell membranes. In contrast to B cell FcR which bound IgG1 preferentially, those on T cells bound both IgG1 and IgG2, raising the possibility that the FcR on T cell is distinct from that on B cell. It is concluded that FcR-bearing T cells represent a subpopulation of cells within the thymus and the secondary lymphoid tissues.", "contents": "A subpopulation of T cells bearing Fc receptors. A wide range of cell populations were examined for Fc receptor (FcR)-bearing T cells: thymus, spleen, peritoneal cells, and T cells activated to H-2 antigens in spleen (ATC spleen) and in thoracic duct lymph (T-TDL). In addition, B lymphocytes from thoracic duct lymph of athymic nude mice and a Thy-1-positive, FcR-positive thymoma served as control cell populations. Reagents used were aggregates of human gamma-globulin and of various mouse myeloma proteins (IgG1, IgG2a, IgG2b), radioiodinated antigen-antibody complexes, and sheep erythrocyte antibody rosettes. Labeling techniques involving radioautography and immunofluorescence were used to demonstrate FcR by one of the above reagents and to identify T cells either by staining with anti-Thy-1.2 or by a specific rabbit anti-mouse T cell serum, or by failure to stain with anti-mouse immunoglobulin. In some experiments phagocytic cells were removed whereas in others they were identified by their capacity to engulf latex particles. Approximately 25% of cells with T cell markers were FcR-bearing cells in thymus, normal spleen, and peritoneal cavity, and 17% in ATC spleen. FcR on T cells in peripheral lymphoid tissues were detectable by aggregates of HGG and myeloma proteins and by radioiodinated immune complexes. Those on T cells in thymus were revealed only by aggregates of HGG. Circulating T cells (T.TDL) failed to display FcR: a) despite the use of a wide range of the above labeling techniques, each of which was shown to detect FcR on other T cells, thymoma cells, and B cells, and b) even after removal of Ig associated with their cell membranes. In contrast to B cell FcR which bound IgG1 preferentially, those on T cells bound both IgG1 and IgG2, raising the possibility that the FcR on T cell is distinct from that on B cell. It is concluded that FcR-bearing T cells represent a subpopulation of cells within the thymus and the secondary lymphoid tissues."}
{"id": "PMID:1100724", "title": "Antigen-enhanced glucosamine incorporation by peritoneal macrophages in cell-mediated hypersensitivity. I. Studies on biology and mechanism.", "content": "The interaction between sensitized lymphocytes and specific antigen occurring in classic delayed hypersensitivity causes guinea pig peritoneal macrophages to incorporate increased amounts of glucosamine into TCA precipitable, membrane-associated, cell surface material. Antigen-induced stimulation of glucosamine also occurred in peritoneal exudate cells (PEC) isolated from animals primed for cutaneous basophil hypersensitivity with certain strong antigens (KLH, vaccinia virus) in incomplete Freund's adjuvant (IFA), and lymphocytes from such animals elaborated MIF when cultured with specific antigen. Thus, the use of complete Freund's adjuvant is not obligatory for the induction of sensitized lymphocytes capable of secreting MIF or stimulating macrophage glucosamine incorporation; however, the potency of the immunogen employed is a critical variable since lymphocytes from animals primed with weaker antigens (HSA, BGG) in IFA did not have these capabilities. Significantly enhanced incorporation of radioactive glucosamine by macrophages occurred when normal PEC were cultured in lymphokine-containing supernatants, but the magnitude of incorporation was smaller than that of sensitized PEC stimulated by antigen. The final 24 hr of macrophage culture was critically important because lymphokines were equally effective in promoting glucosamine incorporation when present for only this interval. The kinetics of this response are thus very similar to those reported for macrophage \"activation\". The mechanism by which sensitized lymphocytes and their products stimulate glucosamine incorporation is not established, but at least part of the increment may be attributed to enhanced transport of glucosamine across the macrophage plasma membrane. The plant lectins Con A and PHA stimulated unsensitized plastic-adherent cells to increased glucosamine in corporation and exerted a further additive stimulation on sensitized PEC when nonadherent sensitized lymphocytes were present. It is likely that these mitogens stimulate glucosamine incorporation by two distinct mechanisms, one involving sensitized nonadherent lymphocytes and a second involving only adherent cells (macrophages and/or plastic adherent lymphocytes.", "contents": "Antigen-enhanced glucosamine incorporation by peritoneal macrophages in cell-mediated hypersensitivity. I. Studies on biology and mechanism. The interaction between sensitized lymphocytes and specific antigen occurring in classic delayed hypersensitivity causes guinea pig peritoneal macrophages to incorporate increased amounts of glucosamine into TCA precipitable, membrane-associated, cell surface material. Antigen-induced stimulation of glucosamine also occurred in peritoneal exudate cells (PEC) isolated from animals primed for cutaneous basophil hypersensitivity with certain strong antigens (KLH, vaccinia virus) in incomplete Freund's adjuvant (IFA), and lymphocytes from such animals elaborated MIF when cultured with specific antigen. Thus, the use of complete Freund's adjuvant is not obligatory for the induction of sensitized lymphocytes capable of secreting MIF or stimulating macrophage glucosamine incorporation; however, the potency of the immunogen employed is a critical variable since lymphocytes from animals primed with weaker antigens (HSA, BGG) in IFA did not have these capabilities. Significantly enhanced incorporation of radioactive glucosamine by macrophages occurred when normal PEC were cultured in lymphokine-containing supernatants, but the magnitude of incorporation was smaller than that of sensitized PEC stimulated by antigen. The final 24 hr of macrophage culture was critically important because lymphokines were equally effective in promoting glucosamine incorporation when present for only this interval. The kinetics of this response are thus very similar to those reported for macrophage \"activation\". The mechanism by which sensitized lymphocytes and their products stimulate glucosamine incorporation is not established, but at least part of the increment may be attributed to enhanced transport of glucosamine across the macrophage plasma membrane. The plant lectins Con A and PHA stimulated unsensitized plastic-adherent cells to increased glucosamine in corporation and exerted a further additive stimulation on sensitized PEC when nonadherent sensitized lymphocytes were present. It is likely that these mitogens stimulate glucosamine incorporation by two distinct mechanisms, one involving sensitized nonadherent lymphocytes and a second involving only adherent cells (macrophages and/or plastic adherent lymphocytes."}
{"id": "PMID:1100725", "title": "Structural and genetic basis of the in vivo immune response to TNP-LPS.", "content": "TNP-lipopolysaccharide (TNP-LPS) is a potent T-independent antigen in vivo, inducing a TNP-PFC response in T-depleted animals. The structural integrity of the lipid A-KDO portion of the LPS carrier molecule appears to be required since the haptenated LPS from Salmonella minnesota Re595 is immunogenic whereas the haptenated derivative of base hydrolyzed LPS is not. The immune response is not associated with any of the common histocompatiblity types, but does depend on the ability of the host strain to respond to LPS. C3H/HeJ mice are not killed by low doses of LPS and give a poor PFC response to TNP-LPS. Lethality and immunogenicity are dominant responses in hybrids of C3H/HeJ and responder mice. The structural and genetic requirements for the response to TNP-LPS suggest an active role for the carrier in the immunogenicity of this T-independent antigen.", "contents": "Structural and genetic basis of the in vivo immune response to TNP-LPS. TNP-lipopolysaccharide (TNP-LPS) is a potent T-independent antigen in vivo, inducing a TNP-PFC response in T-depleted animals. The structural integrity of the lipid A-KDO portion of the LPS carrier molecule appears to be required since the haptenated LPS from Salmonella minnesota Re595 is immunogenic whereas the haptenated derivative of base hydrolyzed LPS is not. The immune response is not associated with any of the common histocompatiblity types, but does depend on the ability of the host strain to respond to LPS. C3H/HeJ mice are not killed by low doses of LPS and give a poor PFC response to TNP-LPS. Lethality and immunogenicity are dominant responses in hybrids of C3H/HeJ and responder mice. The structural and genetic requirements for the response to TNP-LPS suggest an active role for the carrier in the immunogenicity of this T-independent antigen."}
{"id": "PMID:1100726", "title": "Number of histocompatibility loci in Syrian hamsters.", "content": "Syrian hamsters descended from animals captured in the summer of 1971 have been utilized to obtain some estimate of the number of histocompatibility loci in this species. The survival of skin grafts on two F2 populations derived from these animals and two isogenic stocks indicates considerably more histocompatibility loci than previously reported.", "contents": "Number of histocompatibility loci in Syrian hamsters. Syrian hamsters descended from animals captured in the summer of 1971 have been utilized to obtain some estimate of the number of histocompatibility loci in this species. The survival of skin grafts on two F2 populations derived from these animals and two isogenic stocks indicates considerably more histocompatibility loci than previously reported."}
{"id": "PMID:1100727", "title": "The immune adherence receptor: dissociation between the expression of erythrocyte and mononuclear cell C3b receptors.", "content": "Human erythrocytes have a surface receptor for the third component of complement (C3b). This receptor mediates the immune adherence (IA) reaction. In this study, erythrocytes from over 100 normal donors and from donors with rare blood group types were examine for IA reactivity. All donors had red cells with high titer IA activity, with the exception of one Rh null individual whose erythrocytes had barely detectable IA activity. Five other Rh null donors had normal IA titers. Because of the apparent defect in the expression of the red cell C3b receptor in this one individual, the mononuclear cell C3b receptors of this donor were studied and found to be normal. These findings suggest that expression of the erythrocyte C3b receptor may be controlled by factors which differ from those determining expression of the mononuclear cell C3b receptor.", "contents": "The immune adherence receptor: dissociation between the expression of erythrocyte and mononuclear cell C3b receptors. Human erythrocytes have a surface receptor for the third component of complement (C3b). This receptor mediates the immune adherence (IA) reaction. In this study, erythrocytes from over 100 normal donors and from donors with rare blood group types were examine for IA reactivity. All donors had red cells with high titer IA activity, with the exception of one Rh null individual whose erythrocytes had barely detectable IA activity. Five other Rh null donors had normal IA titers. Because of the apparent defect in the expression of the red cell C3b receptor in this one individual, the mononuclear cell C3b receptors of this donor were studied and found to be normal. These findings suggest that expression of the erythrocyte C3b receptor may be controlled by factors which differ from those determining expression of the mononuclear cell C3b receptor."}
{"id": "PMID:1100728", "title": "The humoral response of mouse spleen cells to two types of sheep erythrocytes. I. Genetic control of the response to H and L SRBC.", "content": "Some strains of mice discriminate between two types of sheep erythrocytes (H or L-SRBC) and others do not. It is proposed that there are shared antigen(s) common to both H and L extra antig(s) present only in H. It shown that individual clones of antibody-forming cells make antibody to either the shared or extra antigens. These antigenic differences are not found to correlate with any of the known  major sheep erythrocyte antigens. The genetic control of the ability to discriminate between these two types of red cell was investigated by an analysis of response of F1, F2 and backcross mice. The results suggest that the response is under multigene control. BL/6 mice give high responses to the extra antigens and low responses to the shared (high discriminator). DBA/2 gives high responses to the extra antigen (nondiscriminator). F1 mice give high responses to both extra and shared antigen but still discriminate between the two types of erythrocytes (low discriminator). Additional phenotypes appear in the F2 and backcross mice.", "contents": "The humoral response of mouse spleen cells to two types of sheep erythrocytes. I. Genetic control of the response to H and L SRBC. Some strains of mice discriminate between two types of sheep erythrocytes (H or L-SRBC) and others do not. It is proposed that there are shared antigen(s) common to both H and L extra antig(s) present only in H. It shown that individual clones of antibody-forming cells make antibody to either the shared or extra antigens. These antigenic differences are not found to correlate with any of the known  major sheep erythrocyte antigens. The genetic control of the ability to discriminate between these two types of red cell was investigated by an analysis of response of F1, F2 and backcross mice. The results suggest that the response is under multigene control. BL/6 mice give high responses to the extra antigens and low responses to the shared (high discriminator). DBA/2 gives high responses to the extra antigen (nondiscriminator). F1 mice give high responses to both extra and shared antigen but still discriminate between the two types of erythrocytes (low discriminator). Additional phenotypes appear in the F2 and backcross mice."}
{"id": "PMID:1100729", "title": "Effects of migration inhibiting factor(s) on the in vitro detachment of macrophages.", "content": "Supernatants from spleen cells, derived from mice injected with BCG and cultured with PPD, specificially inhibited the migration of normal mouse peritoneal macrophages when compared with control supernatants. Migration inhibitory supernatants were also shown to decrease the detachment of adherent macrophages in a novel test system. Both macrophage migration and detachment inhibitory activities were abrogated by neuraminidase and chymotrypsin treatment of supernatants but were thermostable, suggesting that the detachment test is a sensitive index of MIF activity.", "contents": "Effects of migration inhibiting factor(s) on the in vitro detachment of macrophages. Supernatants from spleen cells, derived from mice injected with BCG and cultured with PPD, specificially inhibited the migration of normal mouse peritoneal macrophages when compared with control supernatants. Migration inhibitory supernatants were also shown to decrease the detachment of adherent macrophages in a novel test system. Both macrophage migration and detachment inhibitory activities were abrogated by neuraminidase and chymotrypsin treatment of supernatants but were thermostable, suggesting that the detachment test is a sensitive index of MIF activity."}
{"id": "PMID:1100730", "title": "In vivo effects of cortisone on the B cell line in chickens.", "content": "The effects of a single dose of cortisone acetate (5 or 10 mg/100 g body weight) on B cells were examined in young chickens. A dose-dependent increase in numbers of circulating B lymphocytes and a change in their Ig-class distribution were followed by parallel increase in splenic plasma cells and serum immunoglobulins. The higher dose of cortisone produced changes in Bmu and Bgamma cells, whereas the lower dose primarily affected Bmu cells. These steroid-induced changes were preceded by lymphocyte depletion in the cortical regions of bursal follicles, and prior bursectomy prevented steroid-induced increases in circulating B lymphocytes and tissue plasma cells. The results suggest that cortisone can induce bursal lymphocytes to migrate from the bursa and to settle subsequently in peripheral lymphoid tissues where they become mature plasma cells.", "contents": "In vivo effects of cortisone on the B cell line in chickens. The effects of a single dose of cortisone acetate (5 or 10 mg/100 g body weight) on B cells were examined in young chickens. A dose-dependent increase in numbers of circulating B lymphocytes and a change in their Ig-class distribution were followed by parallel increase in splenic plasma cells and serum immunoglobulins. The higher dose of cortisone produced changes in Bmu and Bgamma cells, whereas the lower dose primarily affected Bmu cells. These steroid-induced changes were preceded by lymphocyte depletion in the cortical regions of bursal follicles, and prior bursectomy prevented steroid-induced increases in circulating B lymphocytes and tissue plasma cells. The results suggest that cortisone can induce bursal lymphocytes to migrate from the bursa and to settle subsequently in peripheral lymphoid tissues where they become mature plasma cells."}
{"id": "PMID:1100731", "title": "DNA release as a direct measure of microbial killing. I. Serum bactericidal activity.", "content": "A new method for quantification of microbial killing is presented, based upon the assumption that release of DNA from an organism can be taken as direct evidence of cell death. The assay was applied to measurement of serum bactericidal activity. A \"serum-sensitive\" Escherichia coli, whose DNA was pre-labeled with [14C] thymidine, released 95 to 100% of its radioactivity upon exposure to human serum for 120 min. This was accompanied by a fall in viability with less than 0.1% of the bacteria surviving. Heating serum to 56 degrees C for 30 min completely abolished both DNA release and killing. Normal serum did not release DNA from a \"serum-resistant\". Salmonella typhimurium whereas exposure to ampicillin caused both significant killing and DNA release. This assay is highly specific, sensitive, and rapid.", "contents": "DNA release as a direct measure of microbial killing. I. Serum bactericidal activity. A new method for quantification of microbial killing is presented, based upon the assumption that release of DNA from an organism can be taken as direct evidence of cell death. The assay was applied to measurement of serum bactericidal activity. A \"serum-sensitive\" Escherichia coli, whose DNA was pre-labeled with [14C] thymidine, released 95 to 100% of its radioactivity upon exposure to human serum for 120 min. This was accompanied by a fall in viability with less than 0.1% of the bacteria surviving. Heating serum to 56 degrees C for 30 min completely abolished both DNA release and killing. Normal serum did not release DNA from a \"serum-resistant\". Salmonella typhimurium whereas exposure to ampicillin caused both significant killing and DNA release. This assay is highly specific, sensitive, and rapid."}
{"id": "PMID:1100732", "title": "Effect on tolerance induction of the mode of attachment of the hapten the carrier.", "content": "In this study we have investigated the role played by the mode of attachment of the hapten on autologous IgG in the induction of carrier-determined tolerance. Three conjugates of the dinitrophenyl hapten and mouse gamma-globulin were prepared: 1) DNP-epsilon-lysine-MGG, 2) DNP-azo-MGG (which binds to histidine and tyrosine), 3) DNP-must-MGG (which binds to the carboxyl groups of glutamic or aspartic acid). DNP was also bound to the immunogenic carrier keyhole limpet hemocyanin (KLH) by the three different linkages. The three DNP mouse-gamma-globulin conjugates were used to induce tolerance and immediately thereafter the animals were immunized with  various DNP-KLH conjugates. Antibody response was tested by a modiciation of the Jerne plaque assay. It was found that DNP-epsilon-lysine-MGG induced tolerance to both DNP-epsilon-lysine-KLH and to DNP-azo-KLH. In contrast, neither DNP-azo-MGG nor DNP-must-MGG induced tolerance to DNP-epsilon-lysine-KLH or to DNP-azo-KLH and DNP-must-KLH, respectively. Clearance studies showed no differences in the catabolic rate of the various hapten mouse gamma-globulin conjugates as compared to unconjugated MGG. However, although DNP-must-MGG and DNP-azo-MGG were not tolerogenic, they were immunogenic. In conclusion, this study demonstrated that not only the nature of the carrier but the mode of hapten binding to his carrier are critical for the induction of hapten specific tolerance.", "contents": "Effect on tolerance induction of the mode of attachment of the hapten the carrier. In this study we have investigated the role played by the mode of attachment of the hapten on autologous IgG in the induction of carrier-determined tolerance. Three conjugates of the dinitrophenyl hapten and mouse gamma-globulin were prepared: 1) DNP-epsilon-lysine-MGG, 2) DNP-azo-MGG (which binds to histidine and tyrosine), 3) DNP-must-MGG (which binds to the carboxyl groups of glutamic or aspartic acid). DNP was also bound to the immunogenic carrier keyhole limpet hemocyanin (KLH) by the three different linkages. The three DNP mouse-gamma-globulin conjugates were used to induce tolerance and immediately thereafter the animals were immunized with  various DNP-KLH conjugates. Antibody response was tested by a modiciation of the Jerne plaque assay. It was found that DNP-epsilon-lysine-MGG induced tolerance to both DNP-epsilon-lysine-KLH and to DNP-azo-KLH. In contrast, neither DNP-azo-MGG nor DNP-must-MGG induced tolerance to DNP-epsilon-lysine-KLH or to DNP-azo-KLH and DNP-must-KLH, respectively. Clearance studies showed no differences in the catabolic rate of the various hapten mouse gamma-globulin conjugates as compared to unconjugated MGG. However, although DNP-must-MGG and DNP-azo-MGG were not tolerogenic, they were immunogenic. In conclusion, this study demonstrated that not only the nature of the carrier but the mode of hapten binding to his carrier are critical for the induction of hapten specific tolerance."}
{"id": "PMID:1100733", "title": "In vitro immunogenicity of trinitrophenylated bacteriophage T4. I. Lack of helper cell cooperation.", "content": "A primary immune response was obtained in vitro to trinitrophenylated bacteriophate T4 (TNP-T4) by using normal BALB/c spleen cells challenged in vitro. The primary response was limited to anti-TNP; we failed to find evidence of a primary anti-T4 response in spleen cell cultures challenged with either TNP-T4 or native T4. In vivo priming with the carrier T4 failed to enhance the subsequent in vitro response to TNP-T4 although such priming sometimes caused suppression of the anti-hapten response. The addition of excess free carrier to spleen cell cultures challenged with TNP-T4 failed to suppress the anti-tnp response. Spleen cells treated with anti-theta plus complement were unaffected in their ability to respond to TNP-T4. We conclude that TNP-T4 should be classified among the thymus independent antigens.", "contents": "In vitro immunogenicity of trinitrophenylated bacteriophage T4. I. Lack of helper cell cooperation. A primary immune response was obtained in vitro to trinitrophenylated bacteriophate T4 (TNP-T4) by using normal BALB/c spleen cells challenged in vitro. The primary response was limited to anti-TNP; we failed to find evidence of a primary anti-T4 response in spleen cell cultures challenged with either TNP-T4 or native T4. In vivo priming with the carrier T4 failed to enhance the subsequent in vitro response to TNP-T4 although such priming sometimes caused suppression of the anti-hapten response. The addition of excess free carrier to spleen cell cultures challenged with TNP-T4 failed to suppress the anti-tnp response. Spleen cells treated with anti-theta plus complement were unaffected in their ability to respond to TNP-T4. We conclude that TNP-T4 should be classified among the thymus independent antigens."}
{"id": "PMID:1100737", "title": "[Cellular responses to vibration during dental drilling with belt drive motor engine (in vitro) (author's transl)].", "content": "There have been many reports on the pulpal reaction caused by vibration during high speed dental drilling with air turbine hand-piece. It is, however, not clear pulpal responses to vibration of low speed drilling with 7 000 rpm of belt drive motor engine which produces a fundamental vibration of about 170 cps having most unpleasant sensation at vestibular apparatus. Here, cellular responses of L strain cells to vibration derived from 7 000 rpm and 15 000 rpm of belt drive motor engine were observed in vitro system. There is no difference between the both cellular responses to the vibration of 7 000 rpm and 15 000 rpm. Suspended free cells were easily influenced and degenerated by the vibration, and remarkably checked in their cell-multiplication. On the contrary, in the cells adhered to the substratum of glassware, no degeneration and no inhibitory effect were observed. And the cells showed reversible response of cytoplasmic shrinkage only.", "contents": "[Cellular responses to vibration during dental drilling with belt drive motor engine (in vitro) (author's transl)]. There have been many reports on the pulpal reaction caused by vibration during high speed dental drilling with air turbine hand-piece. It is, however, not clear pulpal responses to vibration of low speed drilling with 7 000 rpm of belt drive motor engine which produces a fundamental vibration of about 170 cps having most unpleasant sensation at vestibular apparatus. Here, cellular responses of L strain cells to vibration derived from 7 000 rpm and 15 000 rpm of belt drive motor engine were observed in vitro system. There is no difference between the both cellular responses to the vibration of 7 000 rpm and 15 000 rpm. Suspended free cells were easily influenced and degenerated by the vibration, and remarkably checked in their cell-multiplication. On the contrary, in the cells adhered to the substratum of glassware, no degeneration and no inhibitory effect were observed. And the cells showed reversible response of cytoplasmic shrinkage only."}
{"id": "PMID:1100740", "title": "Inhibition of leukocyte candidacidal activity by serum from patients with disseminated candidiasis.", "content": "The effect of normal serum and serum for seven patients with disseminated candidiasis (DC) on candidacidal activity of normal human neutrophils (PMNL) was studied. PMNL incubated in DC serum had depressed candiacidal activity when compared to studies with normal serum. The candidacidal defect was not related to differences in particle uptake since phagocytic indices were similar with either normal or DC serum. DC serum did not affect PMNL bactericidal activity against Staphylococcus aureus. When Candida albicans (CA) was incubated in either normal or DC sera, washed, and added to PMNL in normal serum, pronounced inhibition of candidacidal activity was seen in all studies with CA previously incubated in DC serum. Dilution of DC serum or absorption of DC serum with heat-killed CA reversed the candidacidal defect. Ultrastructural studies of phagocytized CA demonstrated small (\"tight\") phagolysosomes and a delay in breakdown of an electron-dense coating on CA previously incubated in DC serum when compared to controls. Inhibition of leukocyte candidacidal activity appeared to be related to high titers of IgG antibodies against CA present in DC serum.", "contents": "Inhibition of leukocyte candidacidal activity by serum from patients with disseminated candidiasis. The effect of normal serum and serum for seven patients with disseminated candidiasis (DC) on candidacidal activity of normal human neutrophils (PMNL) was studied. PMNL incubated in DC serum had depressed candiacidal activity when compared to studies with normal serum. The candidacidal defect was not related to differences in particle uptake since phagocytic indices were similar with either normal or DC serum. DC serum did not affect PMNL bactericidal activity against Staphylococcus aureus. When Candida albicans (CA) was incubated in either normal or DC sera, washed, and added to PMNL in normal serum, pronounced inhibition of candidacidal activity was seen in all studies with CA previously incubated in DC serum. Dilution of DC serum or absorption of DC serum with heat-killed CA reversed the candidacidal defect. Ultrastructural studies of phagocytized CA demonstrated small (\"tight\") phagolysosomes and a delay in breakdown of an electron-dense coating on CA previously incubated in DC serum when compared to controls. Inhibition of leukocyte candidacidal activity appeared to be related to high titers of IgG antibodies against CA present in DC serum."}
{"id": "PMID:1100741", "title": "Relation between insulin and glucose flux rates in the dog.", "content": "A constant infusion isotope dilution procedure for measurement of plasma insulin flux rates was performed in acutely eviscerated dogs receiving an infusion of nonlabeled insulin; the calculated rate of insulin flux corresponded closely to that of the administered rate, thus validating the isotope dilution procedure. Flux rates of insulin and glucose were measured simultaneously at different steady levels in intact dogs. There was a linear relationship between plasma insulin concentration and insulin flux rate. In each animal there was also a linear relationship between glucose flux rate and insulin concentration, and between rates of glucose flux and insulin flux, but there was considerable inter-animal variation in the slope of the regression lines.", "contents": "Relation between insulin and glucose flux rates in the dog. A constant infusion isotope dilution procedure for measurement of plasma insulin flux rates was performed in acutely eviscerated dogs receiving an infusion of nonlabeled insulin; the calculated rate of insulin flux corresponded closely to that of the administered rate, thus validating the isotope dilution procedure. Flux rates of insulin and glucose were measured simultaneously at different steady levels in intact dogs. There was a linear relationship between plasma insulin concentration and insulin flux rate. In each animal there was also a linear relationship between glucose flux rate and insulin concentration, and between rates of glucose flux and insulin flux, but there was considerable inter-animal variation in the slope of the regression lines."}
{"id": "PMID:1100743", "title": "The surgical closure of exposed skull.", "content": "The closure of scalp defects is still surrounded by many uncertainties. Free skin graft has proved most successful. Thiersch graft, split skin graft and full thickness skin graft have their indication for use. Thiersch skin graft is used as a temporary dressing, in order to achieve aseptic wound conditions, but cannot be looked upon as a lasting solution cosmetically and functionally. Split skin is used only for the closure of smaller defects. Full thickness skin grafts are most suitable, because they offer a lasting coverage, which meets to all demands. If the outer table of the skull is exposed, multiple bur holes could be placed immediately in cases of fresh injuries, and these facilitate the growth of granulation tissues which nourish the outer table adequately, so that it remains viable. Exposure of the outer table of the skull for a long time leads to necrosis of the superficial layer. It must therefore be removed down to the diploe with a chisel, or brought to early sequestration by means of numerous perforations made with a bur. Skin should not, however, be placed on the exposed diploe immediately, but it should be allowed to granulate, so as to produce a layer of fibrous tissue beneath the grafted full thickness skin. Thus a direct adhesion to the bony underlayer is prevented, and the skin remains movable. The danger of pressure ulcer is also prevented. The figures show the surgical procedure.", "contents": "The surgical closure of exposed skull. The closure of scalp defects is still surrounded by many uncertainties. Free skin graft has proved most successful. Thiersch graft, split skin graft and full thickness skin graft have their indication for use. Thiersch skin graft is used as a temporary dressing, in order to achieve aseptic wound conditions, but cannot be looked upon as a lasting solution cosmetically and functionally. Split skin is used only for the closure of smaller defects. Full thickness skin grafts are most suitable, because they offer a lasting coverage, which meets to all demands. If the outer table of the skull is exposed, multiple bur holes could be placed immediately in cases of fresh injuries, and these facilitate the growth of granulation tissues which nourish the outer table adequately, so that it remains viable. Exposure of the outer table of the skull for a long time leads to necrosis of the superficial layer. It must therefore be removed down to the diploe with a chisel, or brought to early sequestration by means of numerous perforations made with a bur. Skin should not, however, be placed on the exposed diploe immediately, but it should be allowed to granulate, so as to produce a layer of fibrous tissue beneath the grafted full thickness skin. Thus a direct adhesion to the bony underlayer is prevented, and the skin remains movable. The danger of pressure ulcer is also prevented. The figures show the surgical procedure."}
{"id": "PMID:1100744", "title": "Clinical and histological late findings on pedicled skin flaps in the oral cavity.", "content": "Pedicled skin flaps of 22 patients which had been exposed for many years to the environment of the oral cavity were examined clinically and histologically. The results permit statements to be made on the nature of the changes which occur on the surface, and in the histological picture of the graft. The superficial skin changes, as well as the typical histological structures of the external skin are shown in the micro-photographs presented, to be still preserved after many years.", "contents": "Clinical and histological late findings on pedicled skin flaps in the oral cavity. Pedicled skin flaps of 22 patients which had been exposed for many years to the environment of the oral cavity were examined clinically and histologically. The results permit statements to be made on the nature of the changes which occur on the surface, and in the histological picture of the graft. The superficial skin changes, as well as the typical histological structures of the external skin are shown in the micro-photographs presented, to be still preserved after many years."}
{"id": "PMID:1100745", "title": "Post mortem recovery of mandibular osseous graft with cellulose acetate filter. A case report.", "content": "A surgical procedure was performed to re-establish both aesthetics and functional form to a post partial mandibulectomy patient. A particulate, autogenous osseous graft from the hip was supported by a mandibular tantalum splint lined with a cellulose acetate filter. The patient developed metastatic carcinoma in the donor graft site and lung. The mandibular graft and supporting tissue was removed postmortem and examined histologically. It was observed that normal bone reconstruction was taking place and that union of mandibular bone and graft was evident. Secondary squamous cell carcinoma had invaded the symphysis area of the graft. It is also of interest to note that the tumour was mot able to penetrate those areas where the cellulose acetate filter was present.", "contents": "Post mortem recovery of mandibular osseous graft with cellulose acetate filter. A case report. A surgical procedure was performed to re-establish both aesthetics and functional form to a post partial mandibulectomy patient. A particulate, autogenous osseous graft from the hip was supported by a mandibular tantalum splint lined with a cellulose acetate filter. The patient developed metastatic carcinoma in the donor graft site and lung. The mandibular graft and supporting tissue was removed postmortem and examined histologically. It was observed that normal bone reconstruction was taking place and that union of mandibular bone and graft was evident. Secondary squamous cell carcinoma had invaded the symphysis area of the graft. It is also of interest to note that the tumour was mot able to penetrate those areas where the cellulose acetate filter was present."}
{"id": "PMID:1100746", "title": "Therapeutic uses of permanent pervenous atrial pacemakers: A review.", "content": "The available techniques for permanent pervenous atrial pacing have been reviewed with particular emphasis on the coronary vein approach. The major therapeutic uses for permanent atrial pacing include sinus node dysfunction, recurrent ventricular tachyarrhythmias, and intractable supraventricular tachycardias. Special types of atrial pacing involve the use of bifocal atrioventricular sequential pacing, paired atrial pacing, and rapid atrial pacing generators. The potential problems associated with the coronary vein atrial pacing method are discussed.", "contents": "Therapeutic uses of permanent pervenous atrial pacemakers: A review. The available techniques for permanent pervenous atrial pacing have been reviewed with particular emphasis on the coronary vein approach. The major therapeutic uses for permanent atrial pacing include sinus node dysfunction, recurrent ventricular tachyarrhythmias, and intractable supraventricular tachycardias. Special types of atrial pacing involve the use of bifocal atrioventricular sequential pacing, paired atrial pacing, and rapid atrial pacing generators. The potential problems associated with the coronary vein atrial pacing method are discussed."}
{"id": "PMID:1100747", "title": "Localization of prolactin in rat kidney tissue using a double-antibody technique.", "content": "A fluorescein-labelled double-antibody technique was used for the microscopical localization of ovine prolactin in kidney tissue of rats previously injected intravenously with 50 i.u. of the hormone at varying intervals before death. Thirty seconds after the injection, the hormone could already be visualized in teh lumina of the proximal tubules. After 2 min, discrete praticulate fluorescence appeared on the brush border of the epithelium of the proximal tubules. Three minutes later prolactin was clearly localized in the apical portion of the cytoplasm of these cells as well as on the brush border. Ten minutes after the injection, the hormone appeared exclusively within the cell, highest concentrations being observed in the perinuclear region. After 20 min, however, the hormone appeared evenly distributed throughout the cytoplasm. The distributtion of prolactin was in all cases confined to the proximal tubular region of the nephron. The findings support the suggestion that the site of action of prolactin in the kidney is the epithelium of the proximal tubule. As prolactin was first visualized in the lumen of this portion of the nephron, it appers that the hormone gains access to these cells via the glomerular filtrate.", "contents": "Localization of prolactin in rat kidney tissue using a double-antibody technique. A fluorescein-labelled double-antibody technique was used for the microscopical localization of ovine prolactin in kidney tissue of rats previously injected intravenously with 50 i.u. of the hormone at varying intervals before death. Thirty seconds after the injection, the hormone could already be visualized in teh lumina of the proximal tubules. After 2 min, discrete praticulate fluorescence appeared on the brush border of the epithelium of the proximal tubules. Three minutes later prolactin was clearly localized in the apical portion of the cytoplasm of these cells as well as on the brush border. Ten minutes after the injection, the hormone appeared exclusively within the cell, highest concentrations being observed in the perinuclear region. After 20 min, however, the hormone appeared evenly distributed throughout the cytoplasm. The distributtion of prolactin was in all cases confined to the proximal tubular region of the nephron. The findings support the suggestion that the site of action of prolactin in the kidney is the epithelium of the proximal tubule. As prolactin was first visualized in the lumen of this portion of the nephron, it appers that the hormone gains access to these cells via the glomerular filtrate."}
{"id": "PMID:1100748", "title": "Serum luteininzing hormone and follicle-stimulating hormone concentrations in immature femal rats treated with multiple injections and various  amounts of luteinizing hormone releasing hormone.", "content": "Release of immunoreactive LH and FSH was induced in immature intact female rats by repeated injections of synthetic luteinizing hormone releasing hormone (LH-RH). Altering the dose of LH-RH (5, 10, 20, 50 ng) and the frequency of administration (every 10, 20, 30 or 60 min) over a period of 2 h produced a variety of serum LH and FSH concentrations and ratios. When the dose was a constant 20 ng but the frequency of injections was either 20 or 30 min, a steady state in serum gonadotrophin concentrations was reached within 1 h and the level remained the same during the second hour. When given every 10 min, 20 ng LH-RH produced a much higher concentration of both LH and FSH during the second hour of stimulation. Examination of the gonadotrophin levels after each injection of LH-RH showed that the pituitary response was variable in spite of a constant stimulus.", "contents": "Serum luteininzing hormone and follicle-stimulating hormone concentrations in immature femal rats treated with multiple injections and various  amounts of luteinizing hormone releasing hormone. Release of immunoreactive LH and FSH was induced in immature intact female rats by repeated injections of synthetic luteinizing hormone releasing hormone (LH-RH). Altering the dose of LH-RH (5, 10, 20, 50 ng) and the frequency of administration (every 10, 20, 30 or 60 min) over a period of 2 h produced a variety of serum LH and FSH concentrations and ratios. When the dose was a constant 20 ng but the frequency of injections was either 20 or 30 min, a steady state in serum gonadotrophin concentrations was reached within 1 h and the level remained the same during the second hour. When given every 10 min, 20 ng LH-RH produced a much higher concentration of both LH and FSH during the second hour of stimulation. Examination of the gonadotrophin levels after each injection of LH-RH showed that the pituitary response was variable in spite of a constant stimulus."}
{"id": "PMID:1100752", "title": "A new method for the production of experimental diabetes in rabbits.", "content": "Oral administration of 250 mg hydrochlorothiazide/kg, with previous i.v. injection of 4 i.u. soluble insulin or s.c. injection of protamine-zinc insulin, has been found to produce stable hyperglycaemia in rabbits irrespective of sex. It is thought that insulin helps the selective action of hydrochlorothiazide on the beta cells by removing the protective glucose barrier. Although stimulation of glucagon or some extra-pancreatic mechanism at the initial stage could not be altogether ruled out, the central action of the drug is supported by the finding that the hydrochlorothiazide-induced hyperglycaemia was lowered by the sulphonylurea derivative, chlorpropamide.", "contents": "A new method for the production of experimental diabetes in rabbits. Oral administration of 250 mg hydrochlorothiazide/kg, with previous i.v. injection of 4 i.u. soluble insulin or s.c. injection of protamine-zinc insulin, has been found to produce stable hyperglycaemia in rabbits irrespective of sex. It is thought that insulin helps the selective action of hydrochlorothiazide on the beta cells by removing the protective glucose barrier. Although stimulation of glucagon or some extra-pancreatic mechanism at the initial stage could not be altogether ruled out, the central action of the drug is supported by the finding that the hydrochlorothiazide-induced hyperglycaemia was lowered by the sulphonylurea derivative, chlorpropamide."}
{"id": "PMID:1100756", "title": "Specificity of oxytocin and vasopressin immunofluorescence.", "content": "A total inhibition of immunofluorescence could not be obtained using the technique of preincubating either anti-vasopressin or anti-oxytocin plasmas with their homologous antigens. An alternative test of specificity was therefore developed. Lysine-vasopressin (LVP), arginine-vasopressin (AVP) or oxytocin were covalently bound to CNB-activated agarose beads. These hormone-coupled beads were then either placed immediately on glass slides and treated in the same way as tissue sections for immunofluorescence, or pre-incubated in test-tubes with the antibodies and then prepared for immunofluorescence. Fluorescence was measured quantitatively using a Leitz orthoplan microscope with epi-illumination and a photometer attachment. Without pre-incubation the anti-oxytocin plasmas produced intensive fluorescence on those beads containing their homologous antigen (oxytocin) but only slight fluorescence with the heterologous antigens (AVP or LVP). Anti-vasopressin plasmas produced intensive fluorescence of AVP-, LVP- and oxytocin-containing beads. Because the neurohypophysial hormones were bound to agarose beads, antibodies binding to these hormones could be removed by simple centrifugation. Anti-oxytocin and anti-vasopressin lost their fluorescence capacity after pre-incubation with oxytocin- or vasopressin-containing beads respectively, showing that all the antibodies important for fluorescence were bound to homologous antigens. Pre-incubation of anti-oxytocin or anti-vasopressin with beads coupled to their heterologous hormones completely removed the components binding to the heterologous hormone, leaving antibodies that showed fluorescence only with oxytocin or vasopressin respectively. This purification showed that in contrast to the findings with homologous antigens, not all of the antibody population bound to its heterologous antigen. Using non-purified anti-vasopressin, immunofluorescence was observed in neurohypophyses of homozygous Brattleboro rats. This was evidently due to those antibodies which bind to oxytocin, since the fluorescence was abolished after pre-incubating the antibody-containing plasmas with oxytocin-coupled beads. Immunofluorescence was still observed, however, in the suprachiasmatic nucleus of both Wistar and heterozygous Brattleboro rats, if purified anti-vasopressin was used. This was probably due to either vasopressin storage or production in these hypothalamic cells.", "contents": "Specificity of oxytocin and vasopressin immunofluorescence. A total inhibition of immunofluorescence could not be obtained using the technique of preincubating either anti-vasopressin or anti-oxytocin plasmas with their homologous antigens. An alternative test of specificity was therefore developed. Lysine-vasopressin (LVP), arginine-vasopressin (AVP) or oxytocin were covalently bound to CNB-activated agarose beads. These hormone-coupled beads were then either placed immediately on glass slides and treated in the same way as tissue sections for immunofluorescence, or pre-incubated in test-tubes with the antibodies and then prepared for immunofluorescence. Fluorescence was measured quantitatively using a Leitz orthoplan microscope with epi-illumination and a photometer attachment. Without pre-incubation the anti-oxytocin plasmas produced intensive fluorescence on those beads containing their homologous antigen (oxytocin) but only slight fluorescence with the heterologous antigens (AVP or LVP). Anti-vasopressin plasmas produced intensive fluorescence of AVP-, LVP- and oxytocin-containing beads. Because the neurohypophysial hormones were bound to agarose beads, antibodies binding to these hormones could be removed by simple centrifugation. Anti-oxytocin and anti-vasopressin lost their fluorescence capacity after pre-incubation with oxytocin- or vasopressin-containing beads respectively, showing that all the antibodies important for fluorescence were bound to homologous antigens. Pre-incubation of anti-oxytocin or anti-vasopressin with beads coupled to their heterologous hormones completely removed the components binding to the heterologous hormone, leaving antibodies that showed fluorescence only with oxytocin or vasopressin respectively. This purification showed that in contrast to the findings with homologous antigens, not all of the antibody population bound to its heterologous antigen. Using non-purified anti-vasopressin, immunofluorescence was observed in neurohypophyses of homozygous Brattleboro rats. This was evidently due to those antibodies which bind to oxytocin, since the fluorescence was abolished after pre-incubating the antibody-containing plasmas with oxytocin-coupled beads. Immunofluorescence was still observed, however, in the suprachiasmatic nucleus of both Wistar and heterozygous Brattleboro rats, if purified anti-vasopressin was used. This was probably due to either vasopressin storage or production in these hypothalamic cells."}
{"id": "PMID:1100758", "title": "[Studies on the origin of various blood cell lines in the salamander Pleurodeles waltlii (author's transl)].", "content": "Grafting of haemopoietic organs was performed at several developmental stages between diploid and autotetraploid individuals of Pleurodeles waltlii. The difference in size between diploid and tetraploid cells is so obvious that their identification is possible by direct microscopic examination. To avoid immunological rejection, the grafts have been made with inbred animals. 2. After grafting the spleen, the host cells are observed to colonize it. This phenomenon is more important with spleens from donors at early stages of development. When the spleen from a late larval stage or a metamorphosed animal is transplanted into another larva, the blood of the latter contains 66 or 72% erythrocytes originating from the graft. 3. Embryonic liver grafting is followed by a colonization of all haemopoietic organs by cells originating from the granulopoietic tissue of the graft. The proportion of grafted blood cells is then very high. A liver graft performed at a larval stage gives qualitatively similar results but the numbers of cells originating from the graft are lower. A survey of intra-cardiac erythropoiesis in these animals shows that it develops from stream blood cells and not from cardiac endothelial cells. 4. After thymus graft, the lymphoid part of the organ is replaced by the host cells. 5. From these results, it is suggested that the granulopoietic liver tissue contains one or several kinds of stem cells which could differentiate into all types of blood cells.", "contents": "[Studies on the origin of various blood cell lines in the salamander Pleurodeles waltlii (author's transl)]. Grafting of haemopoietic organs was performed at several developmental stages between diploid and autotetraploid individuals of Pleurodeles waltlii. The difference in size between diploid and tetraploid cells is so obvious that their identification is possible by direct microscopic examination. To avoid immunological rejection, the grafts have been made with inbred animals. 2. After grafting the spleen, the host cells are observed to colonize it. This phenomenon is more important with spleens from donors at early stages of development. When the spleen from a late larval stage or a metamorphosed animal is transplanted into another larva, the blood of the latter contains 66 or 72% erythrocytes originating from the graft. 3. Embryonic liver grafting is followed by a colonization of all haemopoietic organs by cells originating from the granulopoietic tissue of the graft. The proportion of grafted blood cells is then very high. A liver graft performed at a larval stage gives qualitatively similar results but the numbers of cells originating from the graft are lower. A survey of intra-cardiac erythropoiesis in these animals shows that it develops from stream blood cells and not from cardiac endothelial cells. 4. After thymus graft, the lymphoid part of the organ is replaced by the host cells. 5. From these results, it is suggested that the granulopoietic liver tissue contains one or several kinds of stem cells which could differentiate into all types of blood cells."}
{"id": "PMID:1100759", "title": "Development of mouse-bank vole interspecific chimaeric embryos.", "content": "One bank vole (Clethrionomys glareolus) embryo and two mouse embryos were combined at the 8- to 16-blastomere stage and cultured in vitro for 33-47 h. In 66% of cases single regular blastocysts were formed. The chimaeric composition of blastocysts was confirmed karyologically. Out of the 222 blastocysts transplanted to 49 pseudopregnant mouse recipients, a total of 52 implantations were found in 20 recipients. Among the 52 implantations, 14 contained embryos and the remaining were resorptions. The majority of embryos were abnormal and fell into two categories: (1) groups of cells surrounded by Reichert's membrane and lying freely in a cavity filled with giant trophoblastic cells, (2) small and retarded egg-cylinders usually composed of endoderm and ectoderm only, and containing a proamniotic cavity. The ectoplacental cone of these embryos was poorly developed or lacking altogether. Two normal-looking embryos were recovered on the 9th and 10th day (4-somite and ca. 12-somite stage). Chimaerism of the younger embryo was confirmed karyologically. No evidence of chimaerism was available in the case of older embryo which was examined histologically. Thirteen implantations examined between 11th and 17th day contained only resorptions. It is suggested that the main cause of the heavy mortality of chimaeric embryos is the profound difference in the course of embryogenesis of these two species immediately following implantation.", "contents": "Development of mouse-bank vole interspecific chimaeric embryos. One bank vole (Clethrionomys glareolus) embryo and two mouse embryos were combined at the 8- to 16-blastomere stage and cultured in vitro for 33-47 h. In 66% of cases single regular blastocysts were formed. The chimaeric composition of blastocysts was confirmed karyologically. Out of the 222 blastocysts transplanted to 49 pseudopregnant mouse recipients, a total of 52 implantations were found in 20 recipients. Among the 52 implantations, 14 contained embryos and the remaining were resorptions. The majority of embryos were abnormal and fell into two categories: (1) groups of cells surrounded by Reichert's membrane and lying freely in a cavity filled with giant trophoblastic cells, (2) small and retarded egg-cylinders usually composed of endoderm and ectoderm only, and containing a proamniotic cavity. The ectoplacental cone of these embryos was poorly developed or lacking altogether. Two normal-looking embryos were recovered on the 9th and 10th day (4-somite and ca. 12-somite stage). Chimaerism of the younger embryo was confirmed karyologically. No evidence of chimaerism was available in the case of older embryo which was examined histologically. Thirteen implantations examined between 11th and 17th day contained only resorptions. It is suggested that the main cause of the heavy mortality of chimaeric embryos is the profound difference in the course of embryogenesis of these two species immediately following implantation."}
{"id": "PMID:1100760", "title": "Pathenogenetic activation of mouse oocytes following avertin anaesthesia.", "content": "Avertin anaesthesia induced mouse eggs to become activated parthenogenetically. An increasing incidence of activation was observed when females were anaesthetized 6-5, 9 and 13 h after ovulation, reaching a maximum of 45.8 percent. In a spontaneously ovulating group approximately 12.5 percent of all the eggs ovulated, or 27.3 percent of all the eggs activated evoked a decidual response, and the presence of implanting embryos was confirmed histologically. These findings demonstrate a new and simple method of inducing post-implantation parthenogenetic development in the mouse, and stress the necessity of taking into account the possible consequences of anaesthesia in the early post-ovulatory period.", "contents": "Pathenogenetic activation of mouse oocytes following avertin anaesthesia. Avertin anaesthesia induced mouse eggs to become activated parthenogenetically. An increasing incidence of activation was observed when females were anaesthetized 6-5, 9 and 13 h after ovulation, reaching a maximum of 45.8 percent. In a spontaneously ovulating group approximately 12.5 percent of all the eggs ovulated, or 27.3 percent of all the eggs activated evoked a decidual response, and the presence of implanting embryos was confirmed histologically. These findings demonstrate a new and simple method of inducing post-implantation parthenogenetic development in the mouse, and stress the necessity of taking into account the possible consequences of anaesthesia in the early post-ovulatory period."}
{"id": "PMID:1100761", "title": "Stimulation of murine B lymphocytes by isolated C3b.", "content": "Addition of isolated B3b to murine lymph node cell cultures induced increased DNA synthesis. The stimulation is dependent on the dose of C3b added and is potentiated by fetal calf serum present in the medium. Isolated C3 is less stimulatory than C3b; C3a and C3c had no effect on DNA synthesis in these cultures. 10-20% large immunoglobulin containing blastlike cells developed in lymph node cell cultures stimulated by 50 mug C3b in the presence of 3% fetal calf serum. The stimulation by C3b was observed in cultures of lymph node and spleen cells of several mouse strains including C3H/HeJ and congenitally thymus-deficient (nu/nu) mice. The results suggest that B lymphocytes are the main target of the stimulatory effect of C3b. Two mechanisms which may be involved in the stimulation of lymphocytes by C3b are discussed: (a) cross-linking of receptors on the cell surface or between cells, and (b) the binding of C3b to receptors of B lymphocytes and the formation of the complement enzyme C3bB. The results are compatible with the suggestion that activation of C3 is part of the events triggering the B lymphocytes.", "contents": "Stimulation of murine B lymphocytes by isolated C3b. Addition of isolated B3b to murine lymph node cell cultures induced increased DNA synthesis. The stimulation is dependent on the dose of C3b added and is potentiated by fetal calf serum present in the medium. Isolated C3 is less stimulatory than C3b; C3a and C3c had no effect on DNA synthesis in these cultures. 10-20% large immunoglobulin containing blastlike cells developed in lymph node cell cultures stimulated by 50 mug C3b in the presence of 3% fetal calf serum. The stimulation by C3b was observed in cultures of lymph node and spleen cells of several mouse strains including C3H/HeJ and congenitally thymus-deficient (nu/nu) mice. The results suggest that B lymphocytes are the main target of the stimulatory effect of C3b. Two mechanisms which may be involved in the stimulation of lymphocytes by C3b are discussed: (a) cross-linking of receptors on the cell surface or between cells, and (b) the binding of C3b to receptors of B lymphocytes and the formation of the complement enzyme C3bB. The results are compatible with the suggestion that activation of C3 is part of the events triggering the B lymphocytes."}
{"id": "PMID:1100762", "title": "The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor.", "content": "Utilizing the fluorescence-activated cell sorter (FACS) and washed murine antibody-antigen complexes formed in antibody excess, we have demonstrated the presence of the Fc receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc- T lymphocytes from spleens of mice primed 1 wk or 1 mo previously with keyhole limpet hemocyanin (KLH) revealed that the T cells capable of cooperating with DNP-KLH primed B cells to give an adoptive anti-DNP PFC response do not bear the Fc receptor.", "contents": "The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor. Utilizing the fluorescence-activated cell sorter (FACS) and washed murine antibody-antigen complexes formed in antibody excess, we have demonstrated the presence of the Fc receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc- T lymphocytes from spleens of mice primed 1 wk or 1 mo previously with keyhole limpet hemocyanin (KLH) revealed that the T cells capable of cooperating with DNP-KLH primed B cells to give an adoptive anti-DNP PFC response do not bear the Fc receptor."}
{"id": "PMID:1100763", "title": "Control of bone resorption by hematopoietic tissue. The induction and reversal of congenital osteopetrosis in mice through use of bone marrow and splenic transplants.", "content": "The reciprocal transplantation of hematopoietic tissues was carried out on young, lethally irradiated mice of inbred, microphthalmic stock. The cell infusions prepared from the bone marrow or spleen of a normal littermate fully restored capacity to resorb bone and cartilage in the osteopetrotic recipients. Conversely, cell infusions prepared from the spleen of microphthalmic mice induced osteopetrosis in their irradiated, normal littermates. It is concluded that resorption of skeletal matrix is controlled by migratory cells, possibly osteoclastic progenitors, derived from the myelogenous tissues. No evidence was obtained to suggest that skeletal changes observed in the experimental animals were mediated by a graft-vs.-host reaction. The earliest skeletal changes in the experimental mice were detected 2 wk after onset which may represent the length or time required to replace the osteoclast population of the mouse.", "contents": "Control of bone resorption by hematopoietic tissue. The induction and reversal of congenital osteopetrosis in mice through use of bone marrow and splenic transplants. The reciprocal transplantation of hematopoietic tissues was carried out on young, lethally irradiated mice of inbred, microphthalmic stock. The cell infusions prepared from the bone marrow or spleen of a normal littermate fully restored capacity to resorb bone and cartilage in the osteopetrotic recipients. Conversely, cell infusions prepared from the spleen of microphthalmic mice induced osteopetrosis in their irradiated, normal littermates. It is concluded that resorption of skeletal matrix is controlled by migratory cells, possibly osteoclastic progenitors, derived from the myelogenous tissues. No evidence was obtained to suggest that skeletal changes observed in the experimental animals were mediated by a graft-vs.-host reaction. The earliest skeletal changes in the experimental mice were detected 2 wk after onset which may represent the length or time required to replace the osteoclast population of the mouse."}
{"id": "PMID:1100764", "title": "Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers.", "content": "Further mapping of the mouse T-cell factor specific for poly(Try,Glu)-polyD-LAla--polyLys is reported. It is shown to be a product of the I-A subregion of the H-2 complex by the use of antisera either raised specifically against or made specific, by absorption, for different regions of the H-2 complex. The factor cooperates across allogeneic barriers, e.g., when factor produced by one strian is combined with bone marrow cells of other H-2 incompatible strains.", "contents": "Antigen-specific T-cell factor in cell cooperation. Mapping within the I region of the H-2 complex and ability to cooperate across allogeneic barriers. Further mapping of the mouse T-cell factor specific for poly(Try,Glu)-polyD-LAla--polyLys is reported. It is shown to be a product of the I-A subregion of the H-2 complex by the use of antisera either raised specifically against or made specific, by absorption, for different regions of the H-2 complex. The factor cooperates across allogeneic barriers, e.g., when factor produced by one strian is combined with bone marrow cells of other H-2 incompatible strains."}
{"id": "PMID:1100765", "title": "Detection of non-H2 antigen(s) which, like Ia antigens, are associated with the Fc receptor of B lymphocytes.", "content": "Antibodies contained in an A/J anti-B10 antiserum, when tested on lymphocytes from B10.A mice, were shown to bind to both B and T cells and to inhibit the binding of Ig complexes to the Fc receptors of B lymphocytes. These antibodies could be removed by absorption with B10.D2 lymphocytes. Similar results were obtained with lymphocytes from two to six (B6A)F1 x A/J backcross mice which had H-2a/a genotype. These data indicate that alloantigens determined by at least one non-H-2 locus are associated with or a part of Fc receptors. These antigens may be similar in structure and function to Ia antigens.", "contents": "Detection of non-H2 antigen(s) which, like Ia antigens, are associated with the Fc receptor of B lymphocytes. Antibodies contained in an A/J anti-B10 antiserum, when tested on lymphocytes from B10.A mice, were shown to bind to both B and T cells and to inhibit the binding of Ig complexes to the Fc receptors of B lymphocytes. These antibodies could be removed by absorption with B10.D2 lymphocytes. Similar results were obtained with lymphocytes from two to six (B6A)F1 x A/J backcross mice which had H-2a/a genotype. These data indicate that alloantigens determined by at least one non-H-2 locus are associated with or a part of Fc receptors. These antigens may be similar in structure and function to Ia antigens."}
{"id": "PMID:1100766", "title": "Evidence that insect dorsal unpaired medican (DUM) neurons are octopaminergic.", "content": "The dorsal unpaired median neuron innervating the extensor tibiae (DUMETi) of locusts and grasshoppers, as well as some other DUM neurons, cause long-term inhibition of the slow intrinsic rhythm of contraction of the muscle by releasing a neurohumoral agent. Large, (600 - 1900 A), dense-core vesicles are present in the nerve terminals. The inhibitory action is mimicked by infusioninto the leg of a minute (0.01 ml.) drop of locust saline containing a very low concentration (10(-6)M) of dopamine or noradrenaline, or an extraordinarily low concentration of octopamine (2.5 x 10(-9) M). The amplitude and frequency of the contractions slowly return to normal. Neither the cell body nor the neuron fluoresces with the Falck-Hillarp treatment, even after a period of ligature has caused an accumulation of vesicles. Octopamine does not fluoresce with this treatment. Accordingly, it is suggested that this neuron, and the other efferent DUM neurons, are octopaminergic.?23Author", "contents": "Evidence that insect dorsal unpaired medican (DUM) neurons are octopaminergic. The dorsal unpaired median neuron innervating the extensor tibiae (DUMETi) of locusts and grasshoppers, as well as some other DUM neurons, cause long-term inhibition of the slow intrinsic rhythm of contraction of the muscle by releasing a neurohumoral agent. Large, (600 - 1900 A), dense-core vesicles are present in the nerve terminals. The inhibitory action is mimicked by infusioninto the leg of a minute (0.01 ml.) drop of locust saline containing a very low concentration (10(-6)M) of dopamine or noradrenaline, or an extraordinarily low concentration of octopamine (2.5 x 10(-9) M). The amplitude and frequency of the contractions slowly return to normal. Neither the cell body nor the neuron fluoresces with the Falck-Hillarp treatment, even after a period of ligature has caused an accumulation of vesicles. Octopamine does not fluoresce with this treatment. Accordingly, it is suggested that this neuron, and the other efferent DUM neurons, are octopaminergic.?23Author"}
{"id": "PMID:1100767", "title": "Synthesis of octopamine by insect dorsal median unpaired neurons.", "content": "Dorsal unpaired median (DUM) neurons of locusts and grasshoppers inhibit the instrinsic rhythm of contraction of metathoracic extensor tibia muscle fibers. This physiological action is mimicked by very low concentrations of octopamine and by higher concentrations of dopamine and noradrenaline. We have examined the synthesis of biogenic amines from tritiated tyrosine by these neurons. DUM cell bodies and a peripheral nerve containing the DUM axon which terminates in the extensor tibia both produced octopamine, but neither noradrenaline nor dopamine synthesis was detected. The observations support the suggestion that the dorsal unpaired median neurons are octopaminergic.", "contents": "Synthesis of octopamine by insect dorsal median unpaired neurons. Dorsal unpaired median (DUM) neurons of locusts and grasshoppers inhibit the instrinsic rhythm of contraction of metathoracic extensor tibia muscle fibers. This physiological action is mimicked by very low concentrations of octopamine and by higher concentrations of dopamine and noradrenaline. We have examined the synthesis of biogenic amines from tritiated tyrosine by these neurons. DUM cell bodies and a peripheral nerve containing the DUM axon which terminates in the extensor tibia both produced octopamine, but neither noradrenaline nor dopamine synthesis was detected. The observations support the suggestion that the dorsal unpaired median neurons are octopaminergic."}
{"id": "PMID:1100768", "title": "Atomic absorption analysis of some trace metals of toxicological interest.", "content": "A selective and critical review, mainly from 1971 to 1974, of atomic absorption applied to the determination of eight toxic metals (lead, mercury, cadmium, thallium, arsenic, bismuth, beryllium, and boron) in blood, urine, and tissue is presented. Discussion involves both flame and flameless atomization, sample preparation, matrix modification, background correction, and contamination. Advantages of other atomic spectrometric techniques and use of recent confirmatory instrumental methods are included. Some normal and toxic levels are mentioned.", "contents": "Atomic absorption analysis of some trace metals of toxicological interest. A selective and critical review, mainly from 1971 to 1974, of atomic absorption applied to the determination of eight toxic metals (lead, mercury, cadmium, thallium, arsenic, bismuth, beryllium, and boron) in blood, urine, and tissue is presented. Discussion involves both flame and flameless atomization, sample preparation, matrix modification, background correction, and contamination. Advantages of other atomic spectrometric techniques and use of recent confirmatory instrumental methods are included. Some normal and toxic levels are mentioned."}
{"id": "PMID:1100772", "title": "Temporary expression of flagellar phase-1 in phase-2 clones of diphasic Salmonella.", "content": "Diphasic Salmonella strains, Salmonella typhimurium TM2, S. abony SW803 and their derivatives differing in flagellar shape and antigen type, were found to produce copolymer segments of phase-1 and phase-2 flagellins among flagella in phase 2, except for a strain which is non-flagellate in phase 1. The copolymer segments were not detected in phase-1 clones of any of the strains. The wave-forms of the copolymers are homologous with those of the copolymer filaments obtained by in vitro reconstitution of the corresponding phase-1 and phase-2 flagellins. Thus, in the mutant producing normal flagella in phase 1 and straight ones in phase 2, copolymer segments with curly or small waves appear among the straight filaments. Formation of the copolymers was attributed to temporary derepression of the structural gene for phase-1, flagellin, HI, in phase 2. Copolymerization occurred in a fraction of the phase-2 cell population at late exponential and early stationary phase in nutrient broth cultures. When a phase-2 cell was temporarily derepressed, the copolymers formed almost simultaneously in every growing flagellar filament of the cell. Their formation continued for a short period until the supply of phase-1 flagellin was exhausted after re-establishment of repression. This period was estimated to be 7-7 min on average, fluctuating between 4 and 13 min in a cell population of a straight flagellar mutant whose generation time was 55 min in late exponential phase.", "contents": "Temporary expression of flagellar phase-1 in phase-2 clones of diphasic Salmonella. Diphasic Salmonella strains, Salmonella typhimurium TM2, S. abony SW803 and their derivatives differing in flagellar shape and antigen type, were found to produce copolymer segments of phase-1 and phase-2 flagellins among flagella in phase 2, except for a strain which is non-flagellate in phase 1. The copolymer segments were not detected in phase-1 clones of any of the strains. The wave-forms of the copolymers are homologous with those of the copolymer filaments obtained by in vitro reconstitution of the corresponding phase-1 and phase-2 flagellins. Thus, in the mutant producing normal flagella in phase 1 and straight ones in phase 2, copolymer segments with curly or small waves appear among the straight filaments. Formation of the copolymers was attributed to temporary derepression of the structural gene for phase-1, flagellin, HI, in phase 2. Copolymerization occurred in a fraction of the phase-2 cell population at late exponential and early stationary phase in nutrient broth cultures. When a phase-2 cell was temporarily derepressed, the copolymers formed almost simultaneously in every growing flagellar filament of the cell. Their formation continued for a short period until the supply of phase-1 flagellin was exhausted after re-establishment of repression. This period was estimated to be 7-7 min on average, fluctuating between 4 and 13 min in a cell population of a straight flagellar mutant whose generation time was 55 min in late exponential phase."}
{"id": "PMID:1100773", "title": "Transduction of a Proteus vulgaris strain by a Proteus mirabilis bacteriophage.", "content": "Only Proteus vulgaris strain PV127 out of many P. vulgaris, P. morganii and Providence strains was transduced to kanamycin resistance by high-frequency transducing variants, 5006MHFTk and 5006MHFTak, of phage 5006M, a general transducing phage for P. mirabilis strain PM5006. The phages adsorbed poorly to strain PV127 and did not form plaques. The transduction frequency of PV127 by these phages was 5 x 10(-8)/p.f.u. adsorbed. Phage 5006M increased the transduction frequencies. Abortive transductants were not detected. Transductants segregated kanamycin-sensitive clones at high frequency and this, together with data from the inactivation of transducing activity of lysates by ultraviolet irradiation, indicated that transduction was by lysogenization. The general transducing property of the phages was not expressed in transductions to auxotrophs of PV127. Transductants (type I) resulting from low multiplicities of phage input adsorbed phage to the same extent as PV127. This suggested a defect in the transducing particles (or host) because single phage 5006M infection converted strain PM5006 to non-adsorption of homologous phage. Type I transductants did not liberate phage, suggesting a defective phage maturation function. Transductants (type II) which arose from higher multiplicities of phage input did not adsorb phage, indicating possible heterogeneity among transducing particles. Phage derived from type II transductants adsorbed poorly to PV127 and transduced it to kanamycin resistance at frequencies similar to those of phages 5006MHFTk and 5006MHFTak, ruling out host-controlled modification as a cause of the low transduction frequencies. This phage transduced PM5006 to antibiotic resistance at high frequencies but generalized transduction was again not detected. It was suggested that general transduction could be performed by particles which, due to a different composition and/or mode of chromosomal integration, made material they carried susceptible to host-cell modification.", "contents": "Transduction of a Proteus vulgaris strain by a Proteus mirabilis bacteriophage. Only Proteus vulgaris strain PV127 out of many P. vulgaris, P. morganii and Providence strains was transduced to kanamycin resistance by high-frequency transducing variants, 5006MHFTk and 5006MHFTak, of phage 5006M, a general transducing phage for P. mirabilis strain PM5006. The phages adsorbed poorly to strain PV127 and did not form plaques. The transduction frequency of PV127 by these phages was 5 x 10(-8)/p.f.u. adsorbed. Phage 5006M increased the transduction frequencies. Abortive transductants were not detected. Transductants segregated kanamycin-sensitive clones at high frequency and this, together with data from the inactivation of transducing activity of lysates by ultraviolet irradiation, indicated that transduction was by lysogenization. The general transducing property of the phages was not expressed in transductions to auxotrophs of PV127. Transductants (type I) resulting from low multiplicities of phage input adsorbed phage to the same extent as PV127. This suggested a defect in the transducing particles (or host) because single phage 5006M infection converted strain PM5006 to non-adsorption of homologous phage. Type I transductants did not liberate phage, suggesting a defective phage maturation function. Transductants (type II) which arose from higher multiplicities of phage input did not adsorb phage, indicating possible heterogeneity among transducing particles. Phage derived from type II transductants adsorbed poorly to PV127 and transduced it to kanamycin resistance at frequencies similar to those of phages 5006MHFTk and 5006MHFTak, ruling out host-controlled modification as a cause of the low transduction frequencies. This phage transduced PM5006 to antibiotic resistance at high frequencies but generalized transduction was again not detected. It was suggested that general transduction could be performed by particles which, due to a different composition and/or mode of chromosomal integration, made material they carried susceptible to host-cell modification."}
{"id": "PMID:1100775", "title": "Regulation of fructose uptake by glucose in Escherichia coli.", "content": "A mutant, DAI, has been isolated from the Escherichia coli K12, strain K2. 1t, as a colony resistant to 2-deoxyglucose (DG) when growing on fructose but still sensitive to DG when growing on other sugars. The mutation in DAI specifically affects the catabolite inhibition of fructose utilization by glucose and glucose-6-phosphate; the affected gene (designated cif) is located at min 41 on the E. coli linkage map and is highly co-transducible with the genes that specify the uptake of fructose (ptsF) and enzymic conversion of fructose-1-phosphate to fructose-1,6-bisphosphate (fpk).", "contents": "Regulation of fructose uptake by glucose in Escherichia coli. A mutant, DAI, has been isolated from the Escherichia coli K12, strain K2. 1t, as a colony resistant to 2-deoxyglucose (DG) when growing on fructose but still sensitive to DG when growing on other sugars. The mutation in DAI specifically affects the catabolite inhibition of fructose utilization by glucose and glucose-6-phosphate; the affected gene (designated cif) is located at min 41 on the E. coli linkage map and is highly co-transducible with the genes that specify the uptake of fructose (ptsF) and enzymic conversion of fructose-1-phosphate to fructose-1,6-bisphosphate (fpk)."}
{"id": "PMID:1100777", "title": "2'-Deoxyadenosine and A23187 as agents for inducing synchrony in the budding yeast, Kluyveromyces fragilis.", "content": "The effects of induction synchrony by 2'-deoxyadenosine and the ionophore A23187 were compared with selection synchrony methods in Kluyveromyces fragilis. 2'-Deoxyadenosine synchronizes nuclear division and this is followed by synchronous cell division, but DNA synthesis is not synchronized. The ionophore A23187 also induces synchronous nuclear division and synchronous cell division, but likewise does not synchronize DNA synthesis.", "contents": "2'-Deoxyadenosine and A23187 as agents for inducing synchrony in the budding yeast, Kluyveromyces fragilis. The effects of induction synchrony by 2'-deoxyadenosine and the ionophore A23187 were compared with selection synchrony methods in Kluyveromyces fragilis. 2'-Deoxyadenosine synchronizes nuclear division and this is followed by synchronous cell division, but DNA synthesis is not synchronized. The ionophore A23187 also induces synchronous nuclear division and synchronous cell division, but likewise does not synchronize DNA synthesis."}
{"id": "PMID:1100780", "title": "Human figure drawings in a prospective study of six disorders: hypertension, coronary heart disease, malignant tumor, suicide, mental illness, and emotional disturbance.", "content": "To test the hypothesis that human figures drawn by young adults are potential predictors of future disease states, drawings of 204 former medical students were examined 13 to 23 years later. During the interval, the 102 subjects in the experimental group developed one of six specified disorders, while the 102 subjects in the control groups remained in good health. Drawings were classified in eight categories based primarily on the stance of the figure. Categories of drawings found to be distinctive for the various groups are: healthy control groups: the neutral or uncommitted attitude; hypertension/coronary group: the inviting or input-demanding attitude; malignant tumor group: the attitude of ambivalence or conflict; suicide/mental illness group: the self-related or withdrawal attitude; emotional disturbance group: incomplete figures, bizarre figures, action scenes. Thus the evidence suggests that the stance of the figure drawing reflects the subject's attitude toward the outside world. When the category profiles of groups are compared, the following differences are found at levels of statistical significance: the total experimental group is different from the total control group; the somatic subset, \"Body Takes the Rap\" is different from the psychological subset, \"Psyche Takes the Rap\"; and the hypertension/coronary group, malignant tumor group, and emotional disturbance group are different from their specific control groups. It is concluded that human figure drawings by this population have predictive potential for certain future disease states, and that there are psychological precursors of somatic disease.", "contents": "Human figure drawings in a prospective study of six disorders: hypertension, coronary heart disease, malignant tumor, suicide, mental illness, and emotional disturbance. To test the hypothesis that human figures drawn by young adults are potential predictors of future disease states, drawings of 204 former medical students were examined 13 to 23 years later. During the interval, the 102 subjects in the experimental group developed one of six specified disorders, while the 102 subjects in the control groups remained in good health. Drawings were classified in eight categories based primarily on the stance of the figure. Categories of drawings found to be distinctive for the various groups are: healthy control groups: the neutral or uncommitted attitude; hypertension/coronary group: the inviting or input-demanding attitude; malignant tumor group: the attitude of ambivalence or conflict; suicide/mental illness group: the self-related or withdrawal attitude; emotional disturbance group: incomplete figures, bizarre figures, action scenes. Thus the evidence suggests that the stance of the figure drawing reflects the subject's attitude toward the outside world. When the category profiles of groups are compared, the following differences are found at levels of statistical significance: the total experimental group is different from the total control group; the somatic subset, \"Body Takes the Rap\" is different from the psychological subset, \"Psyche Takes the Rap\"; and the hypertension/coronary group, malignant tumor group, and emotional disturbance group are different from their specific control groups. It is concluded that human figure drawings by this population have predictive potential for certain future disease states, and that there are psychological precursors of somatic disease."}
{"id": "PMID:1100781", "title": "Electroconvulsive therapy and memory.", "content": "Recent research on the effects of electroconvulsive therapy (ECT) on memory is critically reviewed. Despite some inconsistent findings, unilateral nondominant ECT appears to affect verbal memory less than bilateral ECT. Adequate research on multiple monitored ECT is lacking. With few exceptions, the research methodologies for assessing memory have been inadequate. Many studies have confounded learning with retention, and only very recently has long term memory been adequately studied. Standardized assessment procedures for short term and long term memory are needed, in addition to more sophisticated assessment of memory processes, the duration of memory loss, and qualitative aspects of memories.", "contents": "Electroconvulsive therapy and memory. Recent research on the effects of electroconvulsive therapy (ECT) on memory is critically reviewed. Despite some inconsistent findings, unilateral nondominant ECT appears to affect verbal memory less than bilateral ECT. Adequate research on multiple monitored ECT is lacking. With few exceptions, the research methodologies for assessing memory have been inadequate. Many studies have confounded learning with retention, and only very recently has long term memory been adequately studied. Standardized assessment procedures for short term and long term memory are needed, in addition to more sophisticated assessment of memory processes, the duration of memory loss, and qualitative aspects of memories."}
{"id": "PMID:1100782", "title": "Lithium in treatment failures.", "content": "Thirty-five patients with chronic and incapacitating mental illness who had not responded to the usual pharmacological and interactional therapies were treated with lithium. None of these patients appeared to be suffering from manic-depressive illness. If a trial of lithium resulted in unexpected improvement, lithium's contribution was assessed by double blind substitution of a placebo followed by lithium in an A-B-A-B design in which the patient served as his own control. Five patients (14 per cent) improved dramatically; in retrospect, four of these five patients suffered from nonremitting forms of manic-depressive illness, and the fifth patient suffered from a severe obsessive compulsive neurosis. Six other chronically hospitalized patients improved to the point of unexpected discharge. A trial of lithium therapy is recommended for the \"backward\" or intractable patient.", "contents": "Lithium in treatment failures. Thirty-five patients with chronic and incapacitating mental illness who had not responded to the usual pharmacological and interactional therapies were treated with lithium. None of these patients appeared to be suffering from manic-depressive illness. If a trial of lithium resulted in unexpected improvement, lithium's contribution was assessed by double blind substitution of a placebo followed by lithium in an A-B-A-B design in which the patient served as his own control. Five patients (14 per cent) improved dramatically; in retrospect, four of these five patients suffered from nonremitting forms of manic-depressive illness, and the fifth patient suffered from a severe obsessive compulsive neurosis. Six other chronically hospitalized patients improved to the point of unexpected discharge. A trial of lithium therapy is recommended for the \"backward\" or intractable patient."}
{"id": "PMID:1100784", "title": "Immunofluorescence of vasopressin and oxytocin in the rat hypothalamo-neurohypophypopseal system.", "content": "The present paper deals with the development of an immunofluorescence procedure that allows specific localization of vasopressin and oxytocin in the hypothalamo-neurohypophyseal system(hnx) of the rat. Antibodies against arginine vasopressin (AVP), lysine-vasopressin (LVP) and oxytocin were raised by injecting these hormones that were covalently bound to thyroglobulin into rabbits. The vasopressin-immunized rabbits showed periods of diabetes insipidus, while histoloty of the \"hns revealed an intact neurosecretory system with signs of increased endogenous hormone synthesis in the supraoptic nucleus and increased release in the neuro-hypophysis of some rabbits. The daily water intake of the oxytocin-immunized rabbits was similar to that of control rabbits. The development of antibodies against vasopressin as measured in the immunofluorescence procedure showed a course that was quite different from the curve of the titer as determined by radioimmunoassay (RIA). Also the specificity of the antibodies used in the immunofluorescence procedure was found to be quite different from their specificity in a RIA system. Potency and specificity of the antibodies have to be studied therefore within the immunofluorescence procedure itself. Using freshly frozen acetone-postfixed hypotalami or pituitaries, no sharp localization of immunofluorescence could be obtained in the HNS. Therefore prefixation was performed. Both, the type and the duration of prefixation revealed quite different results regarding the immunofluorescence in the neurosecretory cell boides in the hypotalamus and of their endings in the neurohypophysis. The best immunofluorescence results were obtained using 6 hours glyoxal-prefixation for the hypothalamus and 24 hours formalin-prefixation for the pituitary. The cross-reaction of the antibodies for oxytocin or vasopressin was tested on synthetic hormones that were bound to CNBR-activated agarose beads and mounted on glass sides. All anti-plasmas showed cross-reaction on beads containing the heterologou- antigen. The plasmas were purified by incubation with  beads containing the heterologous hormone until the cross-reacting component had been removed. Using purified antibodies, the distribution of oxytocin and vasopressin cells within the HNS was investigated. More oxytocin containing cells were localized in the rostral part and more vasopressin in the caudal part of both, the supraoptic (SON) and paraventricular nucleus (PVN). Comparable percentages of oxytocin and vasopressin containing cells were found in the SON and PVN. The absolute amount of oxytocin containing cells was 2.5 times more in the SON than in the PVN, which seems to contradict the \"classical\" view that the PVN predominantly or entirely synthetizes oxytocin. In addition, fluorescence was found using antobodies against vasopressin in the suprachiasmatic nucleus in Wistar rats and heterozygous Brattleboro rats, but not in this nucleus of homozygous Brattleboros.", "contents": "Immunofluorescence of vasopressin and oxytocin in the rat hypothalamo-neurohypophypopseal system. The present paper deals with the development of an immunofluorescence procedure that allows specific localization of vasopressin and oxytocin in the hypothalamo-neurohypophyseal system(hnx) of the rat. Antibodies against arginine vasopressin (AVP), lysine-vasopressin (LVP) and oxytocin were raised by injecting these hormones that were covalently bound to thyroglobulin into rabbits. The vasopressin-immunized rabbits showed periods of diabetes insipidus, while histoloty of the \"hns revealed an intact neurosecretory system with signs of increased endogenous hormone synthesis in the supraoptic nucleus and increased release in the neuro-hypophysis of some rabbits. The daily water intake of the oxytocin-immunized rabbits was similar to that of control rabbits. The development of antibodies against vasopressin as measured in the immunofluorescence procedure showed a course that was quite different from the curve of the titer as determined by radioimmunoassay (RIA). Also the specificity of the antibodies used in the immunofluorescence procedure was found to be quite different from their specificity in a RIA system. Potency and specificity of the antibodies have to be studied therefore within the immunofluorescence procedure itself. Using freshly frozen acetone-postfixed hypotalami or pituitaries, no sharp localization of immunofluorescence could be obtained in the HNS. Therefore prefixation was performed. Both, the type and the duration of prefixation revealed quite different results regarding the immunofluorescence in the neurosecretory cell boides in the hypotalamus and of their endings in the neurohypophysis. The best immunofluorescence results were obtained using 6 hours glyoxal-prefixation for the hypothalamus and 24 hours formalin-prefixation for the pituitary. The cross-reaction of the antibodies for oxytocin or vasopressin was tested on synthetic hormones that were bound to CNBR-activated agarose beads and mounted on glass sides. All anti-plasmas showed cross-reaction on beads containing the heterologou- antigen. The plasmas were purified by incubation with  beads containing the heterologous hormone until the cross-reacting component had been removed. Using purified antibodies, the distribution of oxytocin and vasopressin cells within the HNS was investigated. More oxytocin containing cells were localized in the rostral part and more vasopressin in the caudal part of both, the supraoptic (SON) and paraventricular nucleus (PVN). Comparable percentages of oxytocin and vasopressin containing cells were found in the SON and PVN. The absolute amount of oxytocin containing cells was 2.5 times more in the SON than in the PVN, which seems to contradict the \"classical\" view that the PVN predominantly or entirely synthetizes oxytocin. In addition, fluorescence was found using antobodies against vasopressin in the suprachiasmatic nucleus in Wistar rats and heterozygous Brattleboro rats, but not in this nucleus of homozygous Brattleboros."}
{"id": "PMID:1100787", "title": "Functional imaging of intrarenal blood flow using scintillation camera and computer.", "content": "In order to obtain spatial distribution of an index for regional blood flow at each element on a scintigraphic image of the kidney, we attempted the construction of the so-called functional image. After injecting a single bolus of 133Xe into a renal artery by means of a catheter, this objective was accomplished using digital computer processing for a sequence of scintillation camera recordings of the following washout process from the kidney. This is expressed in a form of matrix of disappearance rate constant. Calculation for the rate constant, the flow index of the functional image, was done using either the least squares (LS) method or height-over-area (H/A) method. Although the former method was considered to be theoretically suitable without undue participation of background activities, the latter was preferred because of stable results for image construction. On reviewing the functional image thus obtained from 22 patients representing a variety of renal diseases, the H/A gave specific information concerning regional distribution of the perfusion integrity mainly related to the cortical part of the kidney. This is often difficult to accomplish utilizing the conventional method of compartmental analysis of the xenon washout curve or selective renal angiography.", "contents": "Functional imaging of intrarenal blood flow using scintillation camera and computer. In order to obtain spatial distribution of an index for regional blood flow at each element on a scintigraphic image of the kidney, we attempted the construction of the so-called functional image. After injecting a single bolus of 133Xe into a renal artery by means of a catheter, this objective was accomplished using digital computer processing for a sequence of scintillation camera recordings of the following washout process from the kidney. This is expressed in a form of matrix of disappearance rate constant. Calculation for the rate constant, the flow index of the functional image, was done using either the least squares (LS) method or height-over-area (H/A) method. Although the former method was considered to be theoretically suitable without undue participation of background activities, the latter was preferred because of stable results for image construction. On reviewing the functional image thus obtained from 22 patients representing a variety of renal diseases, the H/A gave specific information concerning regional distribution of the perfusion integrity mainly related to the cortical part of the kidney. This is often difficult to accomplish utilizing the conventional method of compartmental analysis of the xenon washout curve or selective renal angiography."}
{"id": "PMID:1100788", "title": "Head and brain scan findings in rhinocerebral mucormycosis: case report.", "content": "Brain and bone scan findings in two patients suffering from rhinocerebral mucormycosis following kidney transplantation are presented. Two patients who had had kidney transplants and were sustained for over a month on immunosuppressive drugs developed a rare type of opportunistic infection--mucormycosis. They were examined in various stages of their disease. Special attention was paid to the scintillagraphic findings.", "contents": "Head and brain scan findings in rhinocerebral mucormycosis: case report. Brain and bone scan findings in two patients suffering from rhinocerebral mucormycosis following kidney transplantation are presented. Two patients who had had kidney transplants and were sustained for over a month on immunosuppressive drugs developed a rare type of opportunistic infection--mucormycosis. They were examined in various stages of their disease. Special attention was paid to the scintillagraphic findings."}
{"id": "PMID:1100789", "title": "Lumbar myelography with meglumine iocarmate and metrizamide.", "content": "Metrizamide and meglumine iocarmate (Dimer-X), which at present are the contrast media for myelography best tolerated by central nervous system, were compared in a double-blind test comprising a total of 86 patients, all with symptoms of lumbar or sacral root involvement. Symptoms appearing were recorded using a standard interview formula 24 hoaurs after myelography. EEG, routine laboratory analyses of CSF and neurologic examinations were also made. In some cases CSF paper electrophoresis, CSF pressure recordings and determination of CSF iodine content were obtained.", "contents": "Lumbar myelography with meglumine iocarmate and metrizamide. Metrizamide and meglumine iocarmate (Dimer-X), which at present are the contrast media for myelography best tolerated by central nervous system, were compared in a double-blind test comprising a total of 86 patients, all with symptoms of lumbar or sacral root involvement. Symptoms appearing were recorded using a standard interview formula 24 hoaurs after myelography. EEG, routine laboratory analyses of CSF and neurologic examinations were also made. In some cases CSF paper electrophoresis, CSF pressure recordings and determination of CSF iodine content were obtained."}
{"id": "PMID:1100790", "title": "Encasement of the spleen: case report.", "content": "In a patient with extensive reticulum cell sarcoma involving the abdomen and thorax, encasement of the spleen was demonstrated by scintillation imaging. The features of this observation, not previously reported, and its diagnostic significance are discussed.", "contents": "Encasement of the spleen: case report. In a patient with extensive reticulum cell sarcoma involving the abdomen and thorax, encasement of the spleen was demonstrated by scintillation imaging. The features of this observation, not previously reported, and its diagnostic significance are discussed."}
{"id": "PMID:1100792", "title": "Temporomandibular joint ankylosis: review of the literature and report of two cases of bilateral involvement.", "content": "The factors predisposing to ankylosis and the probable pathogenesis are discussed, as treatment, both past and present. Arthroplasty with Silastic interposition is presented as an acceptable means of managing ankylosis, as shown in two case reports. The ultimate postoperative result is dependent on surgical timing, surgical technique, and attention to postsurgical physiotherapy.", "contents": "Temporomandibular joint ankylosis: review of the literature and report of two cases of bilateral involvement. The factors predisposing to ankylosis and the probable pathogenesis are discussed, as treatment, both past and present. Arthroplasty with Silastic interposition is presented as an acceptable means of managing ankylosis, as shown in two case reports. The ultimate postoperative result is dependent on surgical timing, surgical technique, and attention to postsurgical physiotherapy."}
{"id": "PMID:1100793", "title": "Mycoplasma pneumoniae infections and exanthems.", "content": "A review of the medical literature and two case reports of M. pneumoniae infections with exanthems are presented. Erythematous maculopapular and vesicular exanthems were most common. The duration of rash was more than seven days in the majority of instances, and most patients had associated pneumonia. A striking difference in prevalence and clinical symptomatology by sex was noted; 16 of 20 patients analyzed were males, and they frequently dad severe mucocutaneous syndromes. In contrast, severe conjunctivitis, generalized ulcerative stomatitis, and vesicular or bullous exanthems were not seen in females. Clinicians should suspect infection with M. pneumoniae in patients with exanthem and pneumonia, although other etiologic possibilities should also be considered.", "contents": "Mycoplasma pneumoniae infections and exanthems. A review of the medical literature and two case reports of M. pneumoniae infections with exanthems are presented. Erythematous maculopapular and vesicular exanthems were most common. The duration of rash was more than seven days in the majority of instances, and most patients had associated pneumonia. A striking difference in prevalence and clinical symptomatology by sex was noted; 16 of 20 patients analyzed were males, and they frequently dad severe mucocutaneous syndromes. In contrast, severe conjunctivitis, generalized ulcerative stomatitis, and vesicular or bullous exanthems were not seen in females. Clinicians should suspect infection with M. pneumoniae in patients with exanthem and pneumonia, although other etiologic possibilities should also be considered."}
{"id": "PMID:1100794", "title": "Nonimmune rosette formation: a measure of the newborn infant's cellular immune response.", "content": "Nonimmune rosette formation, an in vitro measure of cellular immunity, was evaluated in normal newborn infants. Active rosette formation in 14 specimens of cord blood was 18.9 +/- 4.8% compared to 28.1 +/- 5.2% in 15 adult control samples (p less than 0.05). Total rosette formation in 13 cord blood samples was 33.3 +/- 7.6% compared to 55.1 +/- 6.5% in 15 adult control specimens (p less than 0.05). Tritiated thymidine uptake from phytohemagglutinin stimulation was comparable in cord blood and adult control lymphocytes. The importance of these findings is discussed in light of other recent reports suggesting that cord blood thymic derived T lymphocytes may have reduced immune capability when compared to adult lymphocytes.", "contents": "Nonimmune rosette formation: a measure of the newborn infant's cellular immune response. Nonimmune rosette formation, an in vitro measure of cellular immunity, was evaluated in normal newborn infants. Active rosette formation in 14 specimens of cord blood was 18.9 +/- 4.8% compared to 28.1 +/- 5.2% in 15 adult control samples (p less than 0.05). Total rosette formation in 13 cord blood samples was 33.3 +/- 7.6% compared to 55.1 +/- 6.5% in 15 adult control specimens (p less than 0.05). Tritiated thymidine uptake from phytohemagglutinin stimulation was comparable in cord blood and adult control lymphocytes. The importance of these findings is discussed in light of other recent reports suggesting that cord blood thymic derived T lymphocytes may have reduced immune capability when compared to adult lymphocytes."}
{"id": "PMID:1100802", "title": "The effect of an insoluble foreign body in the pathogenesis of early periodontal disease in the Syrian hamster.", "content": "The effect of adding 15% talc to either a standard periodontal disease-producing diet (Diet 2000) or a laboratory chow (Purina) was studied in the Syrian golden hamster. The additive was found to reduce the amount of periodontal disease with Diet 2000 and also change the characteristics of the plaque. The addition of talc to laboratory chow increased the severity of normally occurring periodontal disease, although not to the level of Diet 2000.", "contents": "The effect of an insoluble foreign body in the pathogenesis of early periodontal disease in the Syrian hamster. The effect of adding 15% talc to either a standard periodontal disease-producing diet (Diet 2000) or a laboratory chow (Purina) was studied in the Syrian golden hamster. The additive was found to reduce the amount of periodontal disease with Diet 2000 and also change the characteristics of the plaque. The addition of talc to laboratory chow increased the severity of normally occurring periodontal disease, although not to the level of Diet 2000."}
{"id": "PMID:1100807", "title": "The importance of the Hanau formula in construction of complete dentures.", "content": "The casts of five patients whose kinematic mandibular axes had been previously located and tatooed on the face were mounted on six articulators (two of Denar D4-A, two of Whip-Mix, and two of Hanau model 130-28). The horizontal and lateral condylar guidances of all articulators were adjusted by using 15 interocclusal records (five protrusive and 10 right and left lateral) made in cold-curing acrylic resin. The adjustments for each articulator were repeated 20 times within five days. The lateral condylar guidances as adjusted by the interocclusal records were compared with those established by Hanau's formula. A significant difference in the means of the condylar readings occurred when the Hanau articulator was adjusted with a lateral interocclusal record and the Hanau formula. The range of means of lateral condylar guidances of Hanau articulators using the Hanau formula was small. This small possibility of variation in the lateral condylar guidance would suggest the use of lateral interocclusal records when precise restorative procedures are necessary. The Hanau formula is the only formula available at this time to determine the average lateral condylar guidance as related to the horizontal condylar guidance.", "contents": "The importance of the Hanau formula in construction of complete dentures. The casts of five patients whose kinematic mandibular axes had been previously located and tatooed on the face were mounted on six articulators (two of Denar D4-A, two of Whip-Mix, and two of Hanau model 130-28). The horizontal and lateral condylar guidances of all articulators were adjusted by using 15 interocclusal records (five protrusive and 10 right and left lateral) made in cold-curing acrylic resin. The adjustments for each articulator were repeated 20 times within five days. The lateral condylar guidances as adjusted by the interocclusal records were compared with those established by Hanau's formula. A significant difference in the means of the condylar readings occurred when the Hanau articulator was adjusted with a lateral interocclusal record and the Hanau formula. The range of means of lateral condylar guidances of Hanau articulators using the Hanau formula was small. This small possibility of variation in the lateral condylar guidance would suggest the use of lateral interocclusal records when precise restorative procedures are necessary. The Hanau formula is the only formula available at this time to determine the average lateral condylar guidance as related to the horizontal condylar guidance."}
{"id": "PMID:1100809", "title": "Guiding planes in clasp reciprocation and retention.", "content": "Answers to two questions were sought: Is there a proper length for a guide plane and does the relationship of the guide plane to the undercut enhance retention? The cylindrical sleeve makes it possible to measure suprabulge contours in relationship to undercuts, thus establishing the \"action distance\" of the retentive arm. The reciprocating distance traveled by the reciprocal part of the clasp must be equal to the action distance: action distance = reciprocation distance = length of guide plane. Three sets of ivorine teeth were used with cast clasps with a connector and with a single clasp to test this concept of intrinsic retention--\"intrinsic retention\" being a guide plane opposed by an undercut at 180 degrees.", "contents": "Guiding planes in clasp reciprocation and retention. Answers to two questions were sought: Is there a proper length for a guide plane and does the relationship of the guide plane to the undercut enhance retention? The cylindrical sleeve makes it possible to measure suprabulge contours in relationship to undercuts, thus establishing the \"action distance\" of the retentive arm. The reciprocating distance traveled by the reciprocal part of the clasp must be equal to the action distance: action distance = reciprocation distance = length of guide plane. Three sets of ivorine teeth were used with cast clasps with a connector and with a single clasp to test this concept of intrinsic retention--\"intrinsic retention\" being a guide plane opposed by an undercut at 180 degrees."}
{"id": "PMID:1100810", "title": "Physical and mechanical properties of nystatin-containing denture liners.", "content": "The mechanical and physical effects of the presence of nystatin in three denture liners were investigated. In general, the modified materials were softer, showed greater strain, and demonstrated permanent set under compression for the duration of the tests. However, the degree of change in properties appeared unlikely to lead to reduced clinical performance. The most significant change was a great increase in the equilibrium capacity for water in the case of the modified liners. This may enhance the pharmacologic effectiveness of these liners by encouraging the release of nystatin at the tissue surface. Bacteriologic and clinical studies continue to investigate the possibility that modified semipermanent liners (Coe-Supersoft) may reduce the recurrence of denture stomatitis.", "contents": "Physical and mechanical properties of nystatin-containing denture liners. The mechanical and physical effects of the presence of nystatin in three denture liners were investigated. In general, the modified materials were softer, showed greater strain, and demonstrated permanent set under compression for the duration of the tests. However, the degree of change in properties appeared unlikely to lead to reduced clinical performance. The most significant change was a great increase in the equilibrium capacity for water in the case of the modified liners. This may enhance the pharmacologic effectiveness of these liners by encouraging the release of nystatin at the tissue surface. Bacteriologic and clinical studies continue to investigate the possibility that modified semipermanent liners (Coe-Supersoft) may reduce the recurrence of denture stomatitis."}
{"id": "PMID:1100811", "title": "Marginal penetration of composite resin restorations as indicated by a tracer dye.", "content": "There was no evidence of marginal penetration of a tracer dye around Class V preparations lined with a BIS-GMA sealant, filled with Nuva-fil (a composite restorative material), and covered with the sealant. In the control group, 72 per cent of the Adaptic restorations showed varying degrees of marginal penetration.", "contents": "Marginal penetration of composite resin restorations as indicated by a tracer dye. There was no evidence of marginal penetration of a tracer dye around Class V preparations lined with a BIS-GMA sealant, filled with Nuva-fil (a composite restorative material), and covered with the sealant. In the control group, 72 per cent of the Adaptic restorations showed varying degrees of marginal penetration."}
{"id": "PMID:1100812", "title": "A direct technique for fabrication of posts and cores.", "content": "A barbed broach covered by cotton fibers is used as a matrix to carry blue inlay wax into the canal prepared for a post. This simple, direct technique of post fabrication lends itself to various clinical situations. The technique requires no special armamentarium.", "contents": "A direct technique for fabrication of posts and cores. A barbed broach covered by cotton fibers is used as a matrix to carry blue inlay wax into the canal prepared for a post. This simple, direct technique of post fabrication lends itself to various clinical situations. The technique requires no special armamentarium."}
{"id": "PMID:1100813", "title": "Matches, clips, needles, or pins.", "content": "Several methods for positioning dowel pins used in the fabrication of removable dies have been presented. Needles, pins, paper clips, hairpins, and matches are all useful in positioning dowel pins in the impression. The use of paper matches is a simple and effective method for holding the dowel pin in the proper relation while the die stone portion of the cast is being poured.", "contents": "Matches, clips, needles, or pins. Several methods for positioning dowel pins used in the fabrication of removable dies have been presented. Needles, pins, paper clips, hairpins, and matches are all useful in positioning dowel pins in the impression. The use of paper matches is a simple and effective method for holding the dowel pin in the proper relation while the die stone portion of the cast is being poured."}
{"id": "PMID:1100823", "title": "Metabolic management of the horse with an acute abdominal crisis.", "content": "The horse with an abdominal crisis caused by acute gastro-intestinal tract obstruction develops hypovolaemia, haemoconcentration, electrolyte depletion, metabolic acidosis and shock. During preparation for operation, treatment with fluids, antibiotics and bicarbonate will impede metabolic imbalance. Stomach decompression may slow the passage of sodium, water and potassium to the gut lumen, reduce pain and minimize the risk of stomach rupture. Selected laboratory determinations and the monitoring of arterial and venous pressures will provide a measure of security, and serve as a guide to replacement therapy. In the post-surgical period, vigilance must be directed towards potassium and bicarbonate imbalance and adequate hydration.", "contents": "Metabolic management of the horse with an acute abdominal crisis. The horse with an abdominal crisis caused by acute gastro-intestinal tract obstruction develops hypovolaemia, haemoconcentration, electrolyte depletion, metabolic acidosis and shock. During preparation for operation, treatment with fluids, antibiotics and bicarbonate will impede metabolic imbalance. Stomach decompression may slow the passage of sodium, water and potassium to the gut lumen, reduce pain and minimize the risk of stomach rupture. Selected laboratory determinations and the monitoring of arterial and venous pressures will provide a measure of security, and serve as a guide to replacement therapy. In the post-surgical period, vigilance must be directed towards potassium and bicarbonate imbalance and adequate hydration."}
{"id": "PMID:1100818", "title": "[A new era in neuroradiology. Computerised axial transverse tomography-C.A.T.T. (Emi-Scanner) and its applications (author's transl)].", "content": "With an adequate follow-up, the authors report their experience with computerised axial transverse tomography (Emi-Scanner) after its use in 500 cases. A description of the method is followed by an account of the material used in this study. They mention the safety of C.A.T.T. and the profound change which this new technique has brought about in the chronology of neuroradiological investigations. The applications of C.A.T.T. are defined in relation to conventional neuroradiological investigations. In traumatic, degenerative, infective, neoplastic and vascular disorders, as well as epilepsy, C.A.T.T. makes possible visualisation of the lesion in the majority of cases. Having dealt with each of these indications, the authors illustrate the typical appearances seen at C.A.T.T. by significant examples. C.A.T.T. reduces the proportion of angiographic and encephalographic examinations. This tendency will become even more marked with the advent of technical development which improve the power of resolution of C.A.T.T.", "contents": "[A new era in neuroradiology. Computerised axial transverse tomography-C.A.T.T. (Emi-Scanner) and its applications (author's transl)]. With an adequate follow-up, the authors report their experience with computerised axial transverse tomography (Emi-Scanner) after its use in 500 cases. A description of the method is followed by an account of the material used in this study. They mention the safety of C.A.T.T. and the profound change which this new technique has brought about in the chronology of neuroradiological investigations. The applications of C.A.T.T. are defined in relation to conventional neuroradiological investigations. In traumatic, degenerative, infective, neoplastic and vascular disorders, as well as epilepsy, C.A.T.T. makes possible visualisation of the lesion in the majority of cases. Having dealt with each of these indications, the authors illustrate the typical appearances seen at C.A.T.T. by significant examples. C.A.T.T. reduces the proportion of angiographic and encephalographic examinations. This tendency will become even more marked with the advent of technical development which improve the power of resolution of C.A.T.T."}
{"id": "PMID:1100824", "title": "Digestive physiology of the horse.", "content": "Recent studies on the digestive physiology of the horse are reviewed. It was suggested that the small intestine is the primary site of digestion and absorption of protein, soluble carbohydrates, most minerals, fats, fat soluble and water soluble vitamins. The large intestine is the primary site of fibre digestion and net water absorption. Significant amounts of phosphorus are also absorbed from the large intestine. Many factors such as rate of passage, processing of feeds, level of intake, work and maturity of plant may influence digestive ability.", "contents": "Digestive physiology of the horse. Recent studies on the digestive physiology of the horse are reviewed. It was suggested that the small intestine is the primary site of digestion and absorption of protein, soluble carbohydrates, most minerals, fats, fat soluble and water soluble vitamins. The large intestine is the primary site of fibre digestion and net water absorption. Significant amounts of phosphorus are also absorbed from the large intestine. Many factors such as rate of passage, processing of feeds, level of intake, work and maturity of plant may influence digestive ability."}
{"id": "PMID:1100819", "title": "[Error introduced in the radiocardiographic measurement of right volume by the non linearity of the detector in high count rates (author's transl)].", "content": "The short life tracers permit the use of high activities and so allow to follow with a good statistic accuracy fast phenomenons such as the different phases of cardiac revolution. However, this advantage may be quickly limited by the dead-time of the detection unit used. It results from this, in high activities, a non linear response which introduces non negligible errors which we have calculated and verifies thanks to experience in the measurement of right ventricular volumes.", "contents": "[Error introduced in the radiocardiographic measurement of right volume by the non linearity of the detector in high count rates (author's transl)]. The short life tracers permit the use of high activities and so allow to follow with a good statistic accuracy fast phenomenons such as the different phases of cardiac revolution. However, this advantage may be quickly limited by the dead-time of the detection unit used. It results from this, in high activities, a non linear response which introduces non negligible errors which we have calculated and verifies thanks to experience in the measurement of right ventricular volumes."}
{"id": "PMID:1100826", "title": "Intestinal absorption of calcium and phosphorus by horses.", "content": "Recent knowledge regarding the absorption of calcium and phosphorus from the intestine of the horse has been reviewed. Consideration has been given to sites, mechanisms and factors affecting absorption as well as to the availability of calcium and phosphorus from feedstuffs commonly used in Eastern North America. Although the anatomy of the digestive tract might appear to impose restrictions on the ability of the horse to assimilate minerals, studies of absorption show that the horse utilizes the calcium and phosphorus of many feeds very efficiently.", "contents": "Intestinal absorption of calcium and phosphorus by horses. Recent knowledge regarding the absorption of calcium and phosphorus from the intestine of the horse has been reviewed. Consideration has been given to sites, mechanisms and factors affecting absorption as well as to the availability of calcium and phosphorus from feedstuffs commonly used in Eastern North America. Although the anatomy of the digestive tract might appear to impose restrictions on the ability of the horse to assimilate minerals, studies of absorption show that the horse utilizes the calcium and phosphorus of many feeds very efficiently."}
{"id": "PMID:1100825", "title": "Carbohydrate digestion and absorption in the equine small intestine.", "content": "Dietary carbohydrates, which constitute a most important source of equine nutrition, are digested and absorbed by a series of complex processes principally in the small intestine, beginning with intraluminal starch hydrolysis by the action of pancreatic amylase. The continuous secretion of a copious volume of pancreatic juice, low in enzyme activity, presumably releases sufficient oligosaccharides for further hydrolysis at the intestinal cell surface by brush border enzymes. Active carrier mediated mechanisms then transport the final hexose products across the intestinal cell for uptake in the hepatic portal system. Brush border disaccharidase activities in the equine small intestine are of the same order of magnitude, and have a similar distribution pattern, to those reported in omnivorous and carnivorous species. The disaccharidase development patterns are characteristic and reflect the ability of the horse to digest the major nutrient sources adequately at various stages of life. The efficiency of the mucosal disaccharidases and the monosaccharide transport systems in the equine small intestine have been established by a series of oral disaccharide and monosaccharide tolerance tests. Horses older than three years of age are unable to hydrolyse lactose, but young and adult horses are fully capable of rapidly hydrolysing sucrose and maltose loads. Several tests have clinical application for assessing small intestinal dysfunction in the investigation of diarrhoea and malabsorption. The deficient digestion or absorption of carbohydrate, whether primary or secondary, can almost always be localized to a defect in the enzymic or transport capacity of the small intestinal surface cell. The continued ingestion of lactose could be detrimental in severely diarrhoeic foals.", "contents": "Carbohydrate digestion and absorption in the equine small intestine. Dietary carbohydrates, which constitute a most important source of equine nutrition, are digested and absorbed by a series of complex processes principally in the small intestine, beginning with intraluminal starch hydrolysis by the action of pancreatic amylase. The continuous secretion of a copious volume of pancreatic juice, low in enzyme activity, presumably releases sufficient oligosaccharides for further hydrolysis at the intestinal cell surface by brush border enzymes. Active carrier mediated mechanisms then transport the final hexose products across the intestinal cell for uptake in the hepatic portal system. Brush border disaccharidase activities in the equine small intestine are of the same order of magnitude, and have a similar distribution pattern, to those reported in omnivorous and carnivorous species. The disaccharidase development patterns are characteristic and reflect the ability of the horse to digest the major nutrient sources adequately at various stages of life. The efficiency of the mucosal disaccharidases and the monosaccharide transport systems in the equine small intestine have been established by a series of oral disaccharide and monosaccharide tolerance tests. Horses older than three years of age are unable to hydrolyse lactose, but young and adult horses are fully capable of rapidly hydrolysing sucrose and maltose loads. Several tests have clinical application for assessing small intestinal dysfunction in the investigation of diarrhoea and malabsorption. The deficient digestion or absorption of carbohydrate, whether primary or secondary, can almost always be localized to a defect in the enzymic or transport capacity of the small intestinal surface cell. The continued ingestion of lactose could be detrimental in severely diarrhoeic foals."}
{"id": "PMID:1100827", "title": "Histological development of the thymic and intestinal lymphoid tissue of the horse.", "content": "The basic components of the immune system, and the defence mechanisms in the gastrointestinal tract, are briefly reviewed. Histological studies in 84 equine foetuses showed that lymphoid cells begin populating the thymic primordium at 11-12 weeks, the mesenteric lymph nodes at 13 weeks, the spleen at 25 weeks and the intestinal lamina propria at 13-14 weeks' gestation. Lymphocytes were seen in the intestinal epithelium very early in gestation. Histological signs of response to antigenic stimulation were seen in five foetuses, indicating that the horse is likely to be capable of mounting an immune response in utero. The changing status of the foal as it becomes fully immunocompetent is discussed.", "contents": "Histological development of the thymic and intestinal lymphoid tissue of the horse. The basic components of the immune system, and the defence mechanisms in the gastrointestinal tract, are briefly reviewed. Histological studies in 84 equine foetuses showed that lymphoid cells begin populating the thymic primordium at 11-12 weeks, the mesenteric lymph nodes at 13 weeks, the spleen at 25 weeks and the intestinal lamina propria at 13-14 weeks' gestation. Lymphocytes were seen in the intestinal epithelium very early in gestation. Histological signs of response to antigenic stimulation were seen in five foetuses, indicating that the horse is likely to be capable of mounting an immune response in utero. The changing status of the foal as it becomes fully immunocompetent is discussed."}
{"id": "PMID:1100828", "title": "Antimalarials. 7.2,8-Bis(trifluoromethyl)-4-quinolinemethanols.", "content": "Based on the high antilalarial activity of alpha-(2-piperidyl)-2,8-bis(trifluoromethyl)-4-quinolinemethanol, ten additional 2,8-bis(trifluoromethyl)-4-quinolinemethanols were prepared in which the amino alcohol side chain was structurally varied. Synthesis of the compounds is described and antimalarial activity data against Plasmodium berghei are presented and discussed in terms of the structure variations.", "contents": "Antimalarials. 7.2,8-Bis(trifluoromethyl)-4-quinolinemethanols. Based on the high antilalarial activity of alpha-(2-piperidyl)-2,8-bis(trifluoromethyl)-4-quinolinemethanol, ten additional 2,8-bis(trifluoromethyl)-4-quinolinemethanols were prepared in which the amino alcohol side chain was structurally varied. Synthesis of the compounds is described and antimalarial activity data against Plasmodium berghei are presented and discussed in terms of the structure variations."}
{"id": "PMID:1100829", "title": "Growth inhibitory properties of aromatic alpha-ketoaldehydes toward bacteria and yeast. Comparison of inhibition and glyoxalase I activity.", "content": "The alpha-ketoaldehydes methylglyoxal and substituted phenylglyoxals are similar in their abilities to inhibit the growth of Escherichia coli and yeast. When logarithmically growing cells are added to media containing 0.3-1 mM alpha-ketoaldehyde, growth stops for several hours, after which normal growth resumes. The period of growth inhibition does not appear to show any correlation with the ability of glyoxalase I to detoxify these alpha-ketoaldehydes. E. coli and yeast glyoxalase I show markedly different substrate specificities. For example, although both enzymes show broad specificity for both aliphatic and aromatic alpha-ketoaldehydes, 2,4,6-trimethylphenylglyoxal is a substrate for the E. coli enzyme but not for the yeast enzyme. Nevertheless, this alpha-ketoaldehyde inhibits the growth of both E. coli and yeast, similar to the other alpha-ketoaldehydes. Enzymes other than glyoxalase I must play a major role in the metabolism of these alpha-ketoaldehydes during the period of growth inhibition.", "contents": "Growth inhibitory properties of aromatic alpha-ketoaldehydes toward bacteria and yeast. Comparison of inhibition and glyoxalase I activity. The alpha-ketoaldehydes methylglyoxal and substituted phenylglyoxals are similar in their abilities to inhibit the growth of Escherichia coli and yeast. When logarithmically growing cells are added to media containing 0.3-1 mM alpha-ketoaldehyde, growth stops for several hours, after which normal growth resumes. The period of growth inhibition does not appear to show any correlation with the ability of glyoxalase I to detoxify these alpha-ketoaldehydes. E. coli and yeast glyoxalase I show markedly different substrate specificities. For example, although both enzymes show broad specificity for both aliphatic and aromatic alpha-ketoaldehydes, 2,4,6-trimethylphenylglyoxal is a substrate for the E. coli enzyme but not for the yeast enzyme. Nevertheless, this alpha-ketoaldehyde inhibits the growth of both E. coli and yeast, similar to the other alpha-ketoaldehydes. Enzymes other than glyoxalase I must play a major role in the metabolism of these alpha-ketoaldehydes during the period of growth inhibition."}
{"id": "PMID:1100836", "title": "Congenital vertebral anomalies: aetiology and relationship to spina bifida cystica.", "content": "A family survey of 337 patients with congenital vertebral anomalies has been carried out from the Scoliosis Clinics of Edinburgh and the Royal National Orthopaedic Hospital, London. From genetic and epidemiological evidence it is clear that multiple vertebral anomalies (without apparent spina bifida) are aetiologically related to anencephaly and spina bifida cystics, carrying a 5-10% risk to subsequent sibs for any one of these defects. The implications for prenatal diagnosis are discussed. Solitary hemivertebrae and localized anterior defects of the vertebral bodies causing kyphoscoliosis are sporadic (non-familial) in nature, carrying no risk to subsequent sibs.", "contents": "Congenital vertebral anomalies: aetiology and relationship to spina bifida cystica. A family survey of 337 patients with congenital vertebral anomalies has been carried out from the Scoliosis Clinics of Edinburgh and the Royal National Orthopaedic Hospital, London. From genetic and epidemiological evidence it is clear that multiple vertebral anomalies (without apparent spina bifida) are aetiologically related to anencephaly and spina bifida cystics, carrying a 5-10% risk to subsequent sibs for any one of these defects. The implications for prenatal diagnosis are discussed. Solitary hemivertebrae and localized anterior defects of the vertebral bodies causing kyphoscoliosis are sporadic (non-familial) in nature, carrying no risk to subsequent sibs."}
{"id": "PMID:1100837", "title": "Adhesion of enteropathogenic Escherichia coli to pig intestinal brush borders: the existence of two pig phenotypes.", "content": "An in-vitro test that demonstrates adhesion of K88-positive Escherichia coli to brush borders prepared from the small intestine of the pig is described. K88-positive E. coli adhered to the brush borders from some pigs (\"positive\" pigs) but not others (\"negative\" pigs). The sires of the pigs tested could be placed into two groups, namely, those that sired only \"positive\" piglets, and those that sired a mixture of \"positive\" and \"negative\" piglets. The incidence of the two phenotypes in litters indicated that \"positive\" and \"negative\" piglets arose as a result of simple Mendelian inheritance. It is suggested that \"negative\" pigs could be bred and that they might have a natural resistance to neonatal infection with K88-positive E. coli.", "contents": "Adhesion of enteropathogenic Escherichia coli to pig intestinal brush borders: the existence of two pig phenotypes. An in-vitro test that demonstrates adhesion of K88-positive Escherichia coli to brush borders prepared from the small intestine of the pig is described. K88-positive E. coli adhered to the brush borders from some pigs (\"positive\" pigs) but not others (\"negative\" pigs). The sires of the pigs tested could be placed into two groups, namely, those that sired only \"positive\" piglets, and those that sired a mixture of \"positive\" and \"negative\" piglets. The incidence of the two phenotypes in litters indicated that \"positive\" and \"negative\" piglets arose as a result of simple Mendelian inheritance. It is suggested that \"negative\" pigs could be bred and that they might have a natural resistance to neonatal infection with K88-positive E. coli."}
{"id": "PMID:1100838", "title": "A method for determining the utilisation of glucose by mycoplasmas.", "content": "A technique for measuring glucose utilisation by mycoplasmas, in which the disappearance of glucose is monitored by the glucose-oxidase method, is proposed as an alternative to the pH method. For maximum reliability the number of organisms in the test suspension must exceed a critical minimum value. Differences in efficiency of glucose utilisation were demonstrated between species of Mycoplasma and Acholeplasma.", "contents": "A method for determining the utilisation of glucose by mycoplasmas. A technique for measuring glucose utilisation by mycoplasmas, in which the disappearance of glucose is monitored by the glucose-oxidase method, is proposed as an alternative to the pH method. For maximum reliability the number of organisms in the test suspension must exceed a critical minimum value. Differences in efficiency of glucose utilisation were demonstrated between species of Mycoplasma and Acholeplasma."}
{"id": "PMID:1100860", "title": "Clinical trials with mebendazole a new broad spectrum anthelmintic.", "content": "This paper presents our experience with mebendazole, a new broad-spectrum anthelmintic, in 91 cases with ascariasis, 71 cases with necatoriasis 54 cases with trichuriasis and 10 cases with T. saginata infection. Various dosage schedules were evaluated. In ascariasis, a single dose of 200 mg was effective in totally clearing the infection in 100 per cent cases. In necatoriasis 200 mg twice daily for four days was found to be the most effective dose. With this dose total eradication of the infection was seen in 92 per cent cases. In trichuriasis, 200 mg twice daily for four days totally eradicated the infection in 90 per cent cases. In T. saginata infection, the drug was totally ineffective. The anthelminitic activity of the drug was demonstrated by the recovery of roundworms, hookworms and whipworms from the stools. The drug was well tolerated. No side effects or toxic effects were seen in any of the cases.", "contents": "Clinical trials with mebendazole a new broad spectrum anthelmintic. This paper presents our experience with mebendazole, a new broad-spectrum anthelmintic, in 91 cases with ascariasis, 71 cases with necatoriasis 54 cases with trichuriasis and 10 cases with T. saginata infection. Various dosage schedules were evaluated. In ascariasis, a single dose of 200 mg was effective in totally clearing the infection in 100 per cent cases. In necatoriasis 200 mg twice daily for four days was found to be the most effective dose. With this dose total eradication of the infection was seen in 92 per cent cases. In trichuriasis, 200 mg twice daily for four days totally eradicated the infection in 90 per cent cases. In T. saginata infection, the drug was totally ineffective. The anthelminitic activity of the drug was demonstrated by the recovery of roundworms, hookworms and whipworms from the stools. The drug was well tolerated. No side effects or toxic effects were seen in any of the cases."}
{"id": "PMID:1100861", "title": "Omental cyst associated with mesenteric tuberculosis.", "content": "A case of omental cyst associated with tuberculosis in a 12 year old Nigerian girl is presented. It is suggested that the omental cyst is lymphogenous in origin and that spread of tuberculosis led to a rapid increase in its size. A brief review of the features of omental cysts is also included.", "contents": "Omental cyst associated with mesenteric tuberculosis. A case of omental cyst associated with tuberculosis in a 12 year old Nigerian girl is presented. It is suggested that the omental cyst is lymphogenous in origin and that spread of tuberculosis led to a rapid increase in its size. A brief review of the features of omental cysts is also included."}
{"id": "PMID:1100862", "title": "Fate of MS2 proteins synthesized in Escherichia coli exposed to amino acid analogues.", "content": "Incorporation of an analoque into MS2 coded proteins prevents the maturation of phages. In addition, there is an alteration in the relative amount of coat protein to replicase protein synthesized, which supports the hypothesis that normal coat protein serves a physiological role as a translation repressor. Further, abnormal proteins, synthesized from the phage genome, are degraded, presumably by a host catabolic system, more rapidly than the normal gene products.", "contents": "Fate of MS2 proteins synthesized in Escherichia coli exposed to amino acid analogues. Incorporation of an analoque into MS2 coded proteins prevents the maturation of phages. In addition, there is an alteration in the relative amount of coat protein to replicase protein synthesized, which supports the hypothesis that normal coat protein serves a physiological role as a translation repressor. Further, abnormal proteins, synthesized from the phage genome, are degraded, presumably by a host catabolic system, more rapidly than the normal gene products."}
{"id": "PMID:1100863", "title": "Degradation of the viral strand of phiX174 parental replicative-form DNA in a rep- host.", "content": "A progressive degradation of the parental viral strand label is observed upon infection of a Rep- mutant of Escherichia coli by 32P-labeled phiX174. Very little parental label remains in the RF (replicative form) by 47 min after infection. Concomitant with this degradation, replicative intermediates are formed which sediment at 21s, the rate of RF I (supercoiled-closed circular DNA), in a neutral sucrose gradient but which denature and sediment in alkaline gradients as single strands of unit size and larger. These denaturable 21s replicative intermediates have been shown previously to be RF molecules containing an elongated viral strand. Addition of chloramphenicol at 7 min after infection at 30 mug/ml, a concentration sufficient to block RF leads to SS (single strand) synthesis but not RF leads to RF synthesis in a wild-type host cell, reduced the amount of viral strand elongation but did not prevent viral strand degradation. The addition of chloramphenicol at 150 mug/ml at 7 min after infection totally prevents both the degradation of the parental label and the formation of the replicative intermediates with elongated tails. We infer that degradation of the viral strand requires the gene A-mediated nicking of the viral strand but not the concomitant elongation of the viral strand.", "contents": "Degradation of the viral strand of phiX174 parental replicative-form DNA in a rep- host. A progressive degradation of the parental viral strand label is observed upon infection of a Rep- mutant of Escherichia coli by 32P-labeled phiX174. Very little parental label remains in the RF (replicative form) by 47 min after infection. Concomitant with this degradation, replicative intermediates are formed which sediment at 21s, the rate of RF I (supercoiled-closed circular DNA), in a neutral sucrose gradient but which denature and sediment in alkaline gradients as single strands of unit size and larger. These denaturable 21s replicative intermediates have been shown previously to be RF molecules containing an elongated viral strand. Addition of chloramphenicol at 7 min after infection at 30 mug/ml, a concentration sufficient to block RF leads to SS (single strand) synthesis but not RF leads to RF synthesis in a wild-type host cell, reduced the amount of viral strand elongation but did not prevent viral strand degradation. The addition of chloramphenicol at 150 mug/ml at 7 min after infection totally prevents both the degradation of the parental label and the formation of the replicative intermediates with elongated tails. We infer that degradation of the viral strand requires the gene A-mediated nicking of the viral strand but not the concomitant elongation of the viral strand."}
{"id": "PMID:1100864", "title": "Characterization of transduction by bacteriophage T1: time of production and density of transducing particles.", "content": "The transducing activity of two different kinds of premature lysates of T1-infected cells have been compared to normal lysates. The results show that T1-transducing particles are made early in the maturation period. The average density of T1-transducing particles is slightly greater than the density of plaque-forming T1.", "contents": "Characterization of transduction by bacteriophage T1: time of production and density of transducing particles. The transducing activity of two different kinds of premature lysates of T1-infected cells have been compared to normal lysates. The results show that T1-transducing particles are made early in the maturation period. The average density of T1-transducing particles is slightly greater than the density of plaque-forming T1."}
{"id": "PMID:1100865", "title": "Phospholipase activity in bacteriophage-infected Escherichia coli. III. Phopholipase A involvement in lysis of T4-infected cells.", "content": "Bacteriophage studies with Escherichia coli K-12 (gamma)DR-DS-, a mutant lacking the major known fatty acyl hydrolases (phospholipases), and its wild-type parent showed equivalent phage infection with regard to phage production and time of phage release. Further examination of the DR-DS- mutant, however, revealed that the progeny bacteriophage were released without complete dissolution of the host cell. Prolonged cell integrity of the infected mutant was noted by spectrophotometry and supported by direct microscope examination. The phage release occurred at normal \"lysis\" time with phage yields comparable to that of the wild-type bacteria. Inner membrane degradation was indicated by the release of beta-galactosidase, a cytoplasmic enzyme, and of trichloracetic acid-precipitable RNA. Thus, outer membrane degradation is required for dissolution of phage-infected cells, and this degradation is at least partly dependent on activation of host phospholipases.", "contents": "Phospholipase activity in bacteriophage-infected Escherichia coli. III. Phopholipase A involvement in lysis of T4-infected cells. Bacteriophage studies with Escherichia coli K-12 (gamma)DR-DS-, a mutant lacking the major known fatty acyl hydrolases (phospholipases), and its wild-type parent showed equivalent phage infection with regard to phage production and time of phage release. Further examination of the DR-DS- mutant, however, revealed that the progeny bacteriophage were released without complete dissolution of the host cell. Prolonged cell integrity of the infected mutant was noted by spectrophotometry and supported by direct microscope examination. The phage release occurred at normal \"lysis\" time with phage yields comparable to that of the wild-type bacteria. Inner membrane degradation was indicated by the release of beta-galactosidase, a cytoplasmic enzyme, and of trichloracetic acid-precipitable RNA. Thus, outer membrane degradation is required for dissolution of phage-infected cells, and this degradation is at least partly dependent on activation of host phospholipases."}
{"id": "PMID:1100871", "title": "Spontaneous reversion of FTA-ABS test reactions.", "content": "In a series of 56 clinically healthy persons with no history of syphilis and negative VDRL tests but reactive fluorescent treponemal antibody absorption (FTA-ABS) tests, 20 spontaneously developed nonreactive FTA-ABS tests in a period of eight months or less. All but one of these had been of 2+ or less fluorescence. In such a situation, the serologic tests for syphilis (VDRL and FTA-ABS tests) should be repeated at intervals for up to a year before coming to a decision regarding the diagnosis of syphilis. Laboratories could facilitate that decision by reporting degrees of fluorescence of the FTA-ABS test.", "contents": "Spontaneous reversion of FTA-ABS test reactions. In a series of 56 clinically healthy persons with no history of syphilis and negative VDRL tests but reactive fluorescent treponemal antibody absorption (FTA-ABS) tests, 20 spontaneously developed nonreactive FTA-ABS tests in a period of eight months or less. All but one of these had been of 2+ or less fluorescence. In such a situation, the serologic tests for syphilis (VDRL and FTA-ABS tests) should be repeated at intervals for up to a year before coming to a decision regarding the diagnosis of syphilis. Laboratories could facilitate that decision by reporting degrees of fluorescence of the FTA-ABS test."}
{"id": "PMID:1100888", "title": "Observations on nonconverting phage, c-n71, obtained from a nontoxigenic strain of Clostridium botulinum type C.", "content": "A nontoxigenic mutant (C-N71) obtained from a toxigenic strain of Clostridium botulinum type C, Stockholm, with nitrosoguanidine treatment was found to be lysogenic by the lysis test. Although the filtrate of a passaged lysate of this nontoxigenic but lysogenic strain, C-N71, lysed cells of the nontoxigenic strain C-AO2 equally as well as the converting phage c-st obtained from the strain C-Stockholm, it did not convert C-AO2 to the toxigenic state. The lysis spectrum of this filtrate was the same as that of the c-st phage. The ability of the filtrate to lyse the indicator cells, C-AO2, was destroyed neither by trypsin nor DNase but was inactivated by heat treatment at 80 C for 10 min. This suggested that the agent which caused lysis was not boticin but probably a phage. An electron micrograph of the complete phage, c-n71, which was similar in morphology to that of the c-st phage was obtained from the filtrate of strain C-N71. Anti-c-n71 phage rabbit serum neutralized both the lytic and the converting activities of the c-st phage. These findings strongly suggest that the c-n71 phage is a mutant of the c-st phage which lacks the gene controlling production of botulinum type C toxin.", "contents": "Observations on nonconverting phage, c-n71, obtained from a nontoxigenic strain of Clostridium botulinum type C. A nontoxigenic mutant (C-N71) obtained from a toxigenic strain of Clostridium botulinum type C, Stockholm, with nitrosoguanidine treatment was found to be lysogenic by the lysis test. Although the filtrate of a passaged lysate of this nontoxigenic but lysogenic strain, C-N71, lysed cells of the nontoxigenic strain C-AO2 equally as well as the converting phage c-st obtained from the strain C-Stockholm, it did not convert C-AO2 to the toxigenic state. The lysis spectrum of this filtrate was the same as that of the c-st phage. The ability of the filtrate to lyse the indicator cells, C-AO2, was destroyed neither by trypsin nor DNase but was inactivated by heat treatment at 80 C for 10 min. This suggested that the agent which caused lysis was not boticin but probably a phage. An electron micrograph of the complete phage, c-n71, which was similar in morphology to that of the c-st phage was obtained from the filtrate of strain C-N71. Anti-c-n71 phage rabbit serum neutralized both the lytic and the converting activities of the c-st phage. These findings strongly suggest that the c-n71 phage is a mutant of the c-st phage which lacks the gene controlling production of botulinum type C toxin."}
{"id": "PMID:1100889", "title": "An amber suppressor of Escherichia coli strain KO1.", "content": "The suppression characteristics of Escherichia coli strain KO1 have been investigated. The growth patterns of nonsense mutants of RNA (GA and f2) and DNA (lambda and T4) phages suggested that KO1 carried an amber, but not ochre or opal suppressors. The comparison of KO1 with previously identified amber suppressors indicated that KO1 differed from su1, su3 and su6 in its suppression pattern. KO1 and su2 shared some properties in common, for instance, their ability to suppress GA amber mutants with one exception (amN20) and the restriction of suppression capacity by the strr mutation. However, the suppression efficiency of KO1 (48%) was about three times that of su2 (18%). A possibility that KO1 contained a new amber suppressor is discussed.", "contents": "An amber suppressor of Escherichia coli strain KO1. The suppression characteristics of Escherichia coli strain KO1 have been investigated. The growth patterns of nonsense mutants of RNA (GA and f2) and DNA (lambda and T4) phages suggested that KO1 carried an amber, but not ochre or opal suppressors. The comparison of KO1 with previously identified amber suppressors indicated that KO1 differed from su1, su3 and su6 in its suppression pattern. KO1 and su2 shared some properties in common, for instance, their ability to suppress GA amber mutants with one exception (amN20) and the restriction of suppression capacity by the strr mutation. However, the suppression efficiency of KO1 (48%) was about three times that of su2 (18%). A possibility that KO1 contained a new amber suppressor is discussed."}
{"id": "PMID:1100890", "title": "Amoxycillin and ampicillin. A comparative study of in vitro sensitivity and induced morphological alterations in Serratia marcescens.", "content": "In vitro antibacterial activities of ampicillin and amoxycillin were compared against pigmented and non-pigmented strains of Serratia marcescens. Ampicillin appeared more effective than amoxycillin; three-fourths of all strains consistently exhibited an ampicillin minimum inhibitory concentration (MIC) of at least one tube less than that recorded for amoxycillin. Complete cross resistance was not observed as has previously been inferred. Further, greater bactericidal activity was demonstrated with ampicillin; minimum bactericidal concentrations (MBC) were either the same as or one tube greater than the MIC. MBC's for amoxycillin, however, were significantly higher; often four to five times greater than the MIC. Ampicillin exhibited greater bactericidal activity as inferred from differences observed in the biological lesions induced, as recorded through observations by scanning electron microscopy (SEM). Spheroplasts were the predominant morphological alteration induced by ampicillin. In contrast, only filament formation, which demonstrated a degree of reversibility, was induced by amoxycillin.", "contents": "Amoxycillin and ampicillin. A comparative study of in vitro sensitivity and induced morphological alterations in Serratia marcescens. In vitro antibacterial activities of ampicillin and amoxycillin were compared against pigmented and non-pigmented strains of Serratia marcescens. Ampicillin appeared more effective than amoxycillin; three-fourths of all strains consistently exhibited an ampicillin minimum inhibitory concentration (MIC) of at least one tube less than that recorded for amoxycillin. Complete cross resistance was not observed as has previously been inferred. Further, greater bactericidal activity was demonstrated with ampicillin; minimum bactericidal concentrations (MBC) were either the same as or one tube greater than the MIC. MBC's for amoxycillin, however, were significantly higher; often four to five times greater than the MIC. Ampicillin exhibited greater bactericidal activity as inferred from differences observed in the biological lesions induced, as recorded through observations by scanning electron microscopy (SEM). Spheroplasts were the predominant morphological alteration induced by ampicillin. In contrast, only filament formation, which demonstrated a degree of reversibility, was induced by amoxycillin."}
{"id": "PMID:1100897", "title": "[Malaria in children, with special reference to cerebral malaria (author's transl)].", "content": "Those who live in areas where Malaria is endemic, acquire immunity by continuous contact. This immunity cannot be acquired during a short holiday. Children in endemic areas acquire a more severe form of malaria during the period of developing immunity and more often suffer complications like acute hemolytic anemia and, in the case of plasmodium falciparum infection, cerebral malaria. This is a report of 39 cases of cerebral malaria which corresponds to an acute encephslopathy with high temperatures, generalized tonic-clonic spasms and unconsciousness. All children were between 6 months and 5 years old. Cerebral malaria at higher ages is rarely seen in Malawi. But its frequency depends on the intensity of endemic infection and the geographic distribution of the types of malaria. 11 (29%) of the 39 children died. Treatment was with chloroquine against which there was no resistance in East Africa for falciparum infections and with plasmaexpanders. In 1 case permanent neurologic changes a spastic cerebral paresis, were seen. Unconsciousness lasting more than 36 hours appears to be a bad prognostic sign. The CSF is clear and normal except for an occasional rise in protein never higher than 90 mg%.", "contents": "[Malaria in children, with special reference to cerebral malaria (author's transl)]. Those who live in areas where Malaria is endemic, acquire immunity by continuous contact. This immunity cannot be acquired during a short holiday. Children in endemic areas acquire a more severe form of malaria during the period of developing immunity and more often suffer complications like acute hemolytic anemia and, in the case of plasmodium falciparum infection, cerebral malaria. This is a report of 39 cases of cerebral malaria which corresponds to an acute encephslopathy with high temperatures, generalized tonic-clonic spasms and unconsciousness. All children were between 6 months and 5 years old. Cerebral malaria at higher ages is rarely seen in Malawi. But its frequency depends on the intensity of endemic infection and the geographic distribution of the types of malaria. 11 (29%) of the 39 children died. Treatment was with chloroquine against which there was no resistance in East Africa for falciparum infections and with plasmaexpanders. In 1 case permanent neurologic changes a spastic cerebral paresis, were seen. Unconsciousness lasting more than 36 hours appears to be a bad prognostic sign. The CSF is clear and normal except for an occasional rise in protein never higher than 90 mg%."}
{"id": "PMID:1100898", "title": "[Supportive care in bone marrow failure. Technical, immunological and clinical advances].", "content": "Supportive care with blood component transfusions has greatly improved prognosis in patients with bone marrow failure. This progress has made possible by newly developed techniques for separation of blood cells and by a better understanding of the antigenicity of human blood cells and of immunologic reactions following their transfusion. Transfusion of white cell and platelet-poor red cell preparations prevent alloimmunization to leukocyte and platelet-bound alloantigens, or non-hemolytic transfusion reactions in already alloimmunized patients. Alloimmunization can be circumvened and effective long-term platelet support to thrombocytopenic patients can be provided by matching donor and recipient for HL-A antigens. The place of granulocyte transfusion in clinical therapy has yet to be defined, although their usefulness in infected granulocytopenic patients is suggested by the few studies reported so far.", "contents": "[Supportive care in bone marrow failure. Technical, immunological and clinical advances]. Supportive care with blood component transfusions has greatly improved prognosis in patients with bone marrow failure. This progress has made possible by newly developed techniques for separation of blood cells and by a better understanding of the antigenicity of human blood cells and of immunologic reactions following their transfusion. Transfusion of white cell and platelet-poor red cell preparations prevent alloimmunization to leukocyte and platelet-bound alloantigens, or non-hemolytic transfusion reactions in already alloimmunized patients. Alloimmunization can be circumvened and effective long-term platelet support to thrombocytopenic patients can be provided by matching donor and recipient for HL-A antigens. The place of granulocyte transfusion in clinical therapy has yet to be defined, although their usefulness in infected granulocytopenic patients is suggested by the few studies reported so far."}
{"id": "PMID:1100899", "title": "Investigations on pituitary and Leydig cell function in chronic hemodialysis and after renal transplantation.", "content": "The hypothalamus-pituitary-Leydig cell axis was investigated in 20 male patients undergoing intermittent hemodialysis and in 21 male patients following renal transplantation compared to normal controls. Plasma testosterone as well as luteinizing hormone and follicle stimulating hormone were determined by radioimmunoassay under basal conditions and after stimulation with choriongonadotropic hormone and hypothalamus releasing hormone respectively. Suppressed Leydig cell function has been demonstrated in dialysed patients as well as in patients after renal transplantation. The Leydig cell insufficiency is more pronounced in the hemodialysed patients. None of these showed testosterone levels in the normal range. They are different in various dialysis schedules. In contrast Leydig cell function is much better in patients after renal transplantation compared to those undergoing intermittent hemodialysis. But even after renal transplantation a diminished Leydig cell function takes place. The degree of Leydig cell insufficiency however varies individually. In a few cases Leydig cell function is restored completely after transplantation. It remains uncertain whether the duration of the posttransplantation period or the function of the graft effects Leydig cell function. Anterior lobe insufficiency has been excluded since plasma concentrations of gonadotropins were slightly elevated before and after LH-RH stimulation as compared to normals.", "contents": "Investigations on pituitary and Leydig cell function in chronic hemodialysis and after renal transplantation. The hypothalamus-pituitary-Leydig cell axis was investigated in 20 male patients undergoing intermittent hemodialysis and in 21 male patients following renal transplantation compared to normal controls. Plasma testosterone as well as luteinizing hormone and follicle stimulating hormone were determined by radioimmunoassay under basal conditions and after stimulation with choriongonadotropic hormone and hypothalamus releasing hormone respectively. Suppressed Leydig cell function has been demonstrated in dialysed patients as well as in patients after renal transplantation. The Leydig cell insufficiency is more pronounced in the hemodialysed patients. None of these showed testosterone levels in the normal range. They are different in various dialysis schedules. In contrast Leydig cell function is much better in patients after renal transplantation compared to those undergoing intermittent hemodialysis. But even after renal transplantation a diminished Leydig cell function takes place. The degree of Leydig cell insufficiency however varies individually. In a few cases Leydig cell function is restored completely after transplantation. It remains uncertain whether the duration of the posttransplantation period or the function of the graft effects Leydig cell function. Anterior lobe insufficiency has been excluded since plasma concentrations of gonadotropins were slightly elevated before and after LH-RH stimulation as compared to normals."}
{"id": "PMID:1100900", "title": "Mechanisms by which tumors avoid destruction by the immune system BCG-catalyzed increase in IgG and IgA blocking activity of lymphocyte-mediated cytotoxicity.", "content": "The immunoglobulin IgM fraction from the serum of one week sarcoma-bearing BALB/c mice increased, the IgG and IgA fractions from the same animal had no effect on the lymphocytemediated cytotoxic release of 51Cr from 51Cr-labelled sarcoma cells. These latter two immunoglobulin fractions from serum of 14 Days, or more, sarcoma-bearing mice inhibited the lymphocyte-mediated cytotoxic activity. The IgA, IgG or IgM fractions from mice inoculated with bacillus Calmette-Guerin (BCG) had no effect, but if these same animals were inoculated with viable sarcoma cells (10(2) to 10(6) cells per mouse) IgG and IgA fractions inhibited the lymphocyte-mediated cytotoxic activity. The magnitude of inhibition was greatest with sera or IgG from tumor-bearing BCG-treated animals.", "contents": "Mechanisms by which tumors avoid destruction by the immune system BCG-catalyzed increase in IgG and IgA blocking activity of lymphocyte-mediated cytotoxicity. The immunoglobulin IgM fraction from the serum of one week sarcoma-bearing BALB/c mice increased, the IgG and IgA fractions from the same animal had no effect on the lymphocytemediated cytotoxic release of 51Cr from 51Cr-labelled sarcoma cells. These latter two immunoglobulin fractions from serum of 14 Days, or more, sarcoma-bearing mice inhibited the lymphocyte-mediated cytotoxic activity. The IgA, IgG or IgM fractions from mice inoculated with bacillus Calmette-Guerin (BCG) had no effect, but if these same animals were inoculated with viable sarcoma cells (10(2) to 10(6) cells per mouse) IgG and IgA fractions inhibited the lymphocyte-mediated cytotoxic activity. The magnitude of inhibition was greatest with sera or IgG from tumor-bearing BCG-treated animals."}
{"id": "PMID:1100912", "title": "The role of the spleen and immunity in the metastatic pattern of a murine reticulum cell sarcoma.", "content": "A reticulum cell sarcoma (RCS) which initially metastasizes selectively to the spleen in C3H/HeN mice has been studied. Previous reports indicated that removal of the spleen results in widespread metastases of this tumor. The current experiments utilized a parabiotic system to determine the effect of additional splenic tissue on the gross and microscopic metastatic pattern of this RCS. The results were that the addition of a second spleen in the parabiotic group was followed by a significant decrease in visceral metastases compared with the splenectomized group. Parabiotic animals with splenectomized partners and sham-operated animals did not significantly differ from control (single) animals, with all three of these groups showing visceral metastatic spread intermediate between the parabiotic and splenectomized groups. The protective effect of additional spleen against visceral metastases was found not to be immunologic, since immunosuppression of mice by irradiation produced a metastatic pattern similar to that of control animals.", "contents": "The role of the spleen and immunity in the metastatic pattern of a murine reticulum cell sarcoma. A reticulum cell sarcoma (RCS) which initially metastasizes selectively to the spleen in C3H/HeN mice has been studied. Previous reports indicated that removal of the spleen results in widespread metastases of this tumor. The current experiments utilized a parabiotic system to determine the effect of additional splenic tissue on the gross and microscopic metastatic pattern of this RCS. The results were that the addition of a second spleen in the parabiotic group was followed by a significant decrease in visceral metastases compared with the splenectomized group. Parabiotic animals with splenectomized partners and sham-operated animals did not significantly differ from control (single) animals, with all three of these groups showing visceral metastatic spread intermediate between the parabiotic and splenectomized groups. The protective effect of additional spleen against visceral metastases was found not to be immunologic, since immunosuppression of mice by irradiation produced a metastatic pattern similar to that of control animals."}
{"id": "PMID:1100913", "title": "Treatment of disseminated cancer by intravenous hydroxyurea and autogenous bone-marrow transplants: experience with 35 patients.", "content": "Massive doses of hydroxyurea (Hydrea) were given to 35 patients suffering from different cancers. Protection to the hematopoietic system was obtained by autogenous marrow transfusions. The clinical response obtained was not considered superior to the results obtained by administering the drug orally over a prolonged period. The described technique is safe and relatively atraumatic, and it may be advisable to administer a large priming dose by this technique to be followed by smaller maintenance doses of a given anticancer chemotherapeutic agent.", "contents": "Treatment of disseminated cancer by intravenous hydroxyurea and autogenous bone-marrow transplants: experience with 35 patients. Massive doses of hydroxyurea (Hydrea) were given to 35 patients suffering from different cancers. Protection to the hematopoietic system was obtained by autogenous marrow transfusions. The clinical response obtained was not considered superior to the results obtained by administering the drug orally over a prolonged period. The described technique is safe and relatively atraumatic, and it may be advisable to administer a large priming dose by this technique to be followed by smaller maintenance doses of a given anticancer chemotherapeutic agent."}
{"id": "PMID:1100914", "title": "Offsetting toxicity of antineoplastic agents.", "content": "PCO, a yeast extract, offsets at least in part the mitotic inhibitory effect of methotrexate and fluorouracil on bone marrow cells in vitro but increases the antimitotic activity of the drugs on ascites Krebs-2 carcinoma under similar conditions. In vivo, PCO enhances the action of methotrexate against the L-1210 lymphoid leukemia and does not interfere with the effectiveness of fluorouracil against the ascites Krebs-2 tumor.", "contents": "Offsetting toxicity of antineoplastic agents. PCO, a yeast extract, offsets at least in part the mitotic inhibitory effect of methotrexate and fluorouracil on bone marrow cells in vitro but increases the antimitotic activity of the drugs on ascites Krebs-2 carcinoma under similar conditions. In vivo, PCO enhances the action of methotrexate against the L-1210 lymphoid leukemia and does not interfere with the effectiveness of fluorouracil against the ascites Krebs-2 tumor."}
{"id": "PMID:1100915", "title": "A clinical trial of CCNU (1-(2 chloroethyl)-3-cyclohexyl-1-nitrosourea) (NSC 79037) in advanced ovarian carcinoma.", "content": "CCNU is a useful drug in treating advanced ovarian carcinoma. Of patients with advanced ovarian carcinoma 37% responded favorably to CCNU. Thrombocytopenia was the primary toxic reaction. Clinical trials are in progress to compare the relative effectiveness, the degree of cross-resistance, and the difference in toxicity between CCNU and alkylating agents.", "contents": "A clinical trial of CCNU (1-(2 chloroethyl)-3-cyclohexyl-1-nitrosourea) (NSC 79037) in advanced ovarian carcinoma. CCNU is a useful drug in treating advanced ovarian carcinoma. Of patients with advanced ovarian carcinoma 37% responded favorably to CCNU. Thrombocytopenia was the primary toxic reaction. Clinical trials are in progress to compare the relative effectiveness, the degree of cross-resistance, and the difference in toxicity between CCNU and alkylating agents."}
{"id": "PMID:1100916", "title": "A predictive test for the selection of cancer chemotherapeutic agents for the treatment of human cancer.", "content": "The use of animals for the screening of chemotherapeutic agents effective against cancer is not always satisfactory. In addition to the problems of cost, space, and care, the results obtained by animal screening do not always parallel those obtained by the clinical use of the agents. With the advent of more refined in vitro technics which can be applied directly to the patient's own tumor tissue, additional information may permit a more critical evaluation of the laboratory findings. The critical index of sensitivity probably lies in the combined evaluation of biochemical analysis and observed morphological damage. From this investigation it would appear that tissue culture of human tumors may serve as a valuable adjunctive method for the screening of chemicals in order to find drugs valuable in the treatment of cancer in man and to serve as a method in the selection of the most effective therapeutic agent for a given tumor in a given patient.", "contents": "A predictive test for the selection of cancer chemotherapeutic agents for the treatment of human cancer. The use of animals for the screening of chemotherapeutic agents effective against cancer is not always satisfactory. In addition to the problems of cost, space, and care, the results obtained by animal screening do not always parallel those obtained by the clinical use of the agents. With the advent of more refined in vitro technics which can be applied directly to the patient's own tumor tissue, additional information may permit a more critical evaluation of the laboratory findings. The critical index of sensitivity probably lies in the combined evaluation of biochemical analysis and observed morphological damage. From this investigation it would appear that tissue culture of human tumors may serve as a valuable adjunctive method for the screening of chemicals in order to find drugs valuable in the treatment of cancer in man and to serve as a method in the selection of the most effective therapeutic agent for a given tumor in a given patient."}
{"id": "PMID:1100917", "title": "Colorectal cancer--prognostic signs in \"local disease\".", "content": "This detailed review of the clincal and pathologic signs which effect the prognosis of patients with colorectal cancer. The following are reviewed: (1) the degree of cellular anaplasia, (2) local penetration of the bowel wall, (3) lymph-node spread, (4) venous invasion, (5) perineural invasion, (6) obstruction and perforation, and (7) the number of cm the lesion is located from the anal verge.", "contents": "Colorectal cancer--prognostic signs in \"local disease\". This detailed review of the clincal and pathologic signs which effect the prognosis of patients with colorectal cancer. The following are reviewed: (1) the degree of cellular anaplasia, (2) local penetration of the bowel wall, (3) lymph-node spread, (4) venous invasion, (5) perineural invasion, (6) obstruction and perforation, and (7) the number of cm the lesion is located from the anal verge."}
{"id": "PMID:1100918", "title": "Acquired ventricular septal defects. Evolution of an operation, surgical technique, and results.", "content": "The pathology and treatment of 19 patients with acquired ventricular septal defects (VSD's) secondary to myocardial infarction are presented. A new method of repair is described: suturing the free right ventricular wall against the VSD to support the friable septum on the right side in continuity with the patch on the left side of the septum. This removes the force of the left ventricle contraction from the patch and septum to the healthy right ventricle wall. Distal cardiac amputation was also used in several instances. The hospital survival rate was 64 per cent. The late functional and hemodynamic results were excellent.", "contents": "Acquired ventricular septal defects. Evolution of an operation, surgical technique, and results. The pathology and treatment of 19 patients with acquired ventricular septal defects (VSD's) secondary to myocardial infarction are presented. A new method of repair is described: suturing the free right ventricular wall against the VSD to support the friable septum on the right side in continuity with the patch on the left side of the septum. This removes the force of the left ventricle contraction from the patch and septum to the healthy right ventricle wall. Distal cardiac amputation was also used in several instances. The hospital survival rate was 64 per cent. The late functional and hemodynamic results were excellent."}
{"id": "PMID:1100919", "title": "The incidence of pathogenic yeasts among open-heart surgery patients-the value of prophylaxis.", "content": "The normal levels of commensal yeasts in patients undergoing open-heart surgery are established and the effect of antifungal prophylaxis is assessed. Mouth swabs and feces were taken for culture from patients on admission to hospital and 1,2, and 3 weeks postoperatively. Eighty-seven patients who received normal treatment and 50 patients who were given oral and topical antifungal prophylaxis commencing 12 days before hospitalization were studied. Yeast pathogens, mainly Candida albicans, were isolated from 42 (48.3 per cent) of the normal group on admission. There was a marked increase in the incidence and quantities of yeasts isolated from patients in the immediate postoperative period. The incidence and levels of yeasts in patients receiving antifungal prophylaxis was considerably reduced both on admission and postoperatively. The risk of Candida sepsis in open-heart surgery patients with high levels of commensal yeasts is discussed and the possibility of routine antifungal prophylaxis raised.", "contents": "The incidence of pathogenic yeasts among open-heart surgery patients-the value of prophylaxis. The normal levels of commensal yeasts in patients undergoing open-heart surgery are established and the effect of antifungal prophylaxis is assessed. Mouth swabs and feces were taken for culture from patients on admission to hospital and 1,2, and 3 weeks postoperatively. Eighty-seven patients who received normal treatment and 50 patients who were given oral and topical antifungal prophylaxis commencing 12 days before hospitalization were studied. Yeast pathogens, mainly Candida albicans, were isolated from 42 (48.3 per cent) of the normal group on admission. There was a marked increase in the incidence and quantities of yeasts isolated from patients in the immediate postoperative period. The incidence and levels of yeasts in patients receiving antifungal prophylaxis was considerably reduced both on admission and postoperatively. The risk of Candida sepsis in open-heart surgery patients with high levels of commensal yeasts is discussed and the possibility of routine antifungal prophylaxis raised."}
{"id": "PMID:1100920", "title": "Unusual complication after total correction of transposition of the great arteries. A case report.", "content": "A patient is described in whom the Mustard operation was carried out with the baffle sutured cephalad to the coronary sinus. This caused severe arterial desaturation which disappeared after the baffle was transposed caudad to the coronary sinus.", "contents": "Unusual complication after total correction of transposition of the great arteries. A case report. A patient is described in whom the Mustard operation was carried out with the baffle sutured cephalad to the coronary sinus. This caused severe arterial desaturation which disappeared after the baffle was transposed caudad to the coronary sinus."}
{"id": "PMID:1100923", "title": "Operative stabilization of nonpenetrating chest injuries.", "content": "Since 1958, a series of 112 patients with severe or moderately severe chest injuries have been treated. An aggressive policy has been adopted toward correcting or preventing major paradoxical chest wall movement by intramedullary pinning of ribs, costal cartilages, and the sternum. Whenever possible, positive-pressure mechanical ventilation and tracheostomy have been avoided. Fifty patients underwent stabilizing operations. The surgical approach was anterolateral in 12 (average 3.3 pins), posterolateral in 35 (average 6.8 pins), and midsternal in 3. Tracheostomy was performed in 8 of these 50 patients. Three died, on the first, third, and twenty-fifth days after injury. The tracheostomy was used only for aspiration of secretions in 3 others and for postoperative intermittent positive-pressure ventilation in 2 others. The duration of intermittent positive-pressure ventilation was 14 days and 1 day, respectively, Orotracheal intubation with positive-pressure mechanical ventilation after operation was required for more than a few hours in 3 patients, 1 of whom died. The 2 survivors were ventilated for 1 and 5 days. There was a total of 11 hospital deaths in these 50 cases. However, in 2 patients the severity of the initial injuries was thought to make death inevitable. Three of the patients who died were over 70 years of age. Operative stabilization permits avoidance or reduction in the duration of tracheostomy and mechanical ventilation. Permanent chest wall deformity is lessened or avoided.", "contents": "Operative stabilization of nonpenetrating chest injuries. Since 1958, a series of 112 patients with severe or moderately severe chest injuries have been treated. An aggressive policy has been adopted toward correcting or preventing major paradoxical chest wall movement by intramedullary pinning of ribs, costal cartilages, and the sternum. Whenever possible, positive-pressure mechanical ventilation and tracheostomy have been avoided. Fifty patients underwent stabilizing operations. The surgical approach was anterolateral in 12 (average 3.3 pins), posterolateral in 35 (average 6.8 pins), and midsternal in 3. Tracheostomy was performed in 8 of these 50 patients. Three died, on the first, third, and twenty-fifth days after injury. The tracheostomy was used only for aspiration of secretions in 3 others and for postoperative intermittent positive-pressure ventilation in 2 others. The duration of intermittent positive-pressure ventilation was 14 days and 1 day, respectively, Orotracheal intubation with positive-pressure mechanical ventilation after operation was required for more than a few hours in 3 patients, 1 of whom died. The 2 survivors were ventilated for 1 and 5 days. There was a total of 11 hospital deaths in these 50 cases. However, in 2 patients the severity of the initial injuries was thought to make death inevitable. Three of the patients who died were over 70 years of age. Operative stabilization permits avoidance or reduction in the duration of tracheostomy and mechanical ventilation. Permanent chest wall deformity is lessened or avoided."}
{"id": "PMID:1101002", "title": "Antarctic medicine, 1775-1975. I.", "content": "An historical review is made of Antarctic medical practice, which is unique because of the absence of an indigenous population. This review begins with the primitive shipboard practice of doctors accompanying Captain James Cook around 1775 and concludes with the modern era of permanent stations and vast scientific endeavour. The heroic era of Scott, Shackleton, Amundsen and Mawson and the highly mechanized transition period are contrasted with the present day. Medical practice on modern expeditions has reached a high standard, but there is still much to be learned concerning human adaptation. Comment is made on the possible utilization of Antarctica's natural resources bringing increases in polar populations and facilitating the expansion of medical research in the future era of polar medicine.", "contents": "Antarctic medicine, 1775-1975. I. An historical review is made of Antarctic medical practice, which is unique because of the absence of an indigenous population. This review begins with the primitive shipboard practice of doctors accompanying Captain James Cook around 1775 and concludes with the modern era of permanent stations and vast scientific endeavour. The heroic era of Scott, Shackleton, Amundsen and Mawson and the highly mechanized transition period are contrasted with the present day. Medical practice on modern expeditions has reached a high standard, but there is still much to be learned concerning human adaptation. Comment is made on the possible utilization of Antarctica's natural resources bringing increases in polar populations and facilitating the expansion of medical research in the future era of polar medicine."}
{"id": "PMID:1100996", "title": "[Disturbance in the equilibrium between DNA and protein synthesis rates in bacteria and their viability].", "content": "The effect of the disturbed correlation between the rates of synthesis of DNA and protein on the viability of bacterial cells was studied with different strain of E. coli K-12. The viability of the cells is determined by their resistance to sharp changes in the rates of synthesis of DNA and protein.", "contents": "[Disturbance in the equilibrium between DNA and protein synthesis rates in bacteria and their viability]. The effect of the disturbed correlation between the rates of synthesis of DNA and protein on the viability of bacterial cells was studied with different strain of E. coli K-12. The viability of the cells is determined by their resistance to sharp changes in the rates of synthesis of DNA and protein."}
{"id": "PMID:1100997", "title": "[Biosynthesis of sterols by various yeasts].", "content": "Biosynthesis of sterines by yeasts was studied with 112 strains belonging to 9 genera; 77 strains synthesized ergosterine and 15 most active strains were selected, which belonged to the following genera: Saccharomyces, Torulopsis, Hanseniaspora, Debaryomyces, Candida, Mechnikowia. Sterine composition was studied in these active strains. The main sterine in all studied strains was ergosterine.", "contents": "[Biosynthesis of sterols by various yeasts]. Biosynthesis of sterines by yeasts was studied with 112 strains belonging to 9 genera; 77 strains synthesized ergosterine and 15 most active strains were selected, which belonged to the following genera: Saccharomyces, Torulopsis, Hanseniaspora, Debaryomyces, Candida, Mechnikowia. Sterine composition was studied in these active strains. The main sterine in all studied strains was ergosterine."}
{"id": "PMID:1100998", "title": "[Physiological and biochemical properties of a Pseudomonas methanolica culture under chemostat cultivation].", "content": "The effect of different concentrations of methanol on Pseudomonas methanolica BKM B-1131 was studied. The limitation of growth by methanol in chemostat was found at a concentration of the substrate below 0.1--0.23 per cent; higher concentrations resulted in the appearance of residual methanol in the medium and in a decrease of the economic coefficient. A declining chemostat curve has been obtained when the growth was limited by a deficiency of methanol. If the growth rate increased, the content of RNA remained the same, the content of protein decreased from 50 to 39 per cent, and the content of POBA increased. Changes in the specific activity of some key enzymes of the Krebs and Embden-Meyerhof cycles and of methanol dehydrogenase suggest an increase in the activity of the Krebs cycle enzymes with the growth rate because at this time the substances between acetate and pyruvate are accumulated which are favourable substrates for the synthesis of POBA.", "contents": "[Physiological and biochemical properties of a Pseudomonas methanolica culture under chemostat cultivation]. The effect of different concentrations of methanol on Pseudomonas methanolica BKM B-1131 was studied. The limitation of growth by methanol in chemostat was found at a concentration of the substrate below 0.1--0.23 per cent; higher concentrations resulted in the appearance of residual methanol in the medium and in a decrease of the economic coefficient. A declining chemostat curve has been obtained when the growth was limited by a deficiency of methanol. If the growth rate increased, the content of RNA remained the same, the content of protein decreased from 50 to 39 per cent, and the content of POBA increased. Changes in the specific activity of some key enzymes of the Krebs and Embden-Meyerhof cycles and of methanol dehydrogenase suggest an increase in the activity of the Krebs cycle enzymes with the growth rate because at this time the substances between acetate and pyruvate are accumulated which are favourable substrates for the synthesis of POBA."}
{"id": "PMID:1100999", "title": "[Autoselection in Candida utilis yeasts in a chemostat culture].", "content": "Long-term continuous cultivation of Candida utilis in the conditions of chemostat, with the growth rate being limited with phosphates, was accompanied by autoselection. The culture obtained had a higher growth rate and differed by some physiological characteristics from the parent culture. The content of intracellular phosphates varied from 1.9 to 0.55% per dry weight, depending on the concentration of phosphates in the growth medium. At the threshold concentration (0.007 g/litre), phosphates were not virtually utilized by the culture. Specific time of transitional processes depends on the value of interfering effect.", "contents": "[Autoselection in Candida utilis yeasts in a chemostat culture]. Long-term continuous cultivation of Candida utilis in the conditions of chemostat, with the growth rate being limited with phosphates, was accompanied by autoselection. The culture obtained had a higher growth rate and differed by some physiological characteristics from the parent culture. The content of intracellular phosphates varied from 1.9 to 0.55% per dry weight, depending on the concentration of phosphates in the growth medium. At the threshold concentration (0.007 g/litre), phosphates were not virtually utilized by the culture. Specific time of transitional processes depends on the value of interfering effect."}
{"id": "PMID:1101000", "title": "[Polyploid hybrids of industrial yeast races and the prospects for using them in production].", "content": "The ploidity of the parent forms, hybrids, and two industrial yeast races was studied by determining the content of DNA per cell, the ratios during allel splitting, the dimensions and volumes of the cells. The triploid nature of the baker's race 14--2 and some hybrids was found (for example, the productive hybrid 112 obtained by crossing the distillery and baker's races). The results obtained suggest the possibility to apply the techniques of hybridization and polyploidy for selecting productive yeast strains.", "contents": "[Polyploid hybrids of industrial yeast races and the prospects for using them in production]. The ploidity of the parent forms, hybrids, and two industrial yeast races was studied by determining the content of DNA per cell, the ratios during allel splitting, the dimensions and volumes of the cells. The triploid nature of the baker's race 14--2 and some hybrids was found (for example, the productive hybrid 112 obtained by crossing the distillery and baker's races). The results obtained suggest the possibility to apply the techniques of hybridization and polyploidy for selecting productive yeast strains."}
{"id": "PMID:1101001", "title": "[Method of isolating pure cultures of mesophilic, thermotolerant and thermophilic methane-utilizing bacteria].", "content": "The paper describes a technique for isolation of pure cultures of various physiological groups (mesophilic, thermotolerant, and thermophilic) of methylotrophs bacteria. The technique is based on the application of solid media containing simultaneously two sources of carbon: elective for the accompanying microflora and elective for the obligate methylotrophs. If the culture to be studied is inoculated into such a medium, the following colonies will be found: (1) the initial culture; (2) pure cultures of the methylotrophs; and (3) the accompanying microflora. The paper presents a scheme for isolation of pure cultures of the methylotrophs, criteria for controlling their purity, recommendations for the composition of growth media which would not inhibit the growth of the obligate methylotrophs by organic substrates. The technique may also be used for the isolation of pure cultures of other bacteria (mycobacteria, lithotrophs, etc.) which are difficult to be separated from the accompanying microflora.", "contents": "[Method of isolating pure cultures of mesophilic, thermotolerant and thermophilic methane-utilizing bacteria]. The paper describes a technique for isolation of pure cultures of various physiological groups (mesophilic, thermotolerant, and thermophilic) of methylotrophs bacteria. The technique is based on the application of solid media containing simultaneously two sources of carbon: elective for the accompanying microflora and elective for the obligate methylotrophs. If the culture to be studied is inoculated into such a medium, the following colonies will be found: (1) the initial culture; (2) pure cultures of the methylotrophs; and (3) the accompanying microflora. The paper presents a scheme for isolation of pure cultures of the methylotrophs, criteria for controlling their purity, recommendations for the composition of growth media which would not inhibit the growth of the obligate methylotrophs by organic substrates. The technique may also be used for the isolation of pure cultures of other bacteria (mycobacteria, lithotrophs, etc.) which are difficult to be separated from the accompanying microflora."}
{"id": "PMID:1101009", "title": "Modification of normal cell surface by smooth membrane preparations from BHK-21 cells infected with Newcastle disease virus.", "content": "Smooth membrane fractions were prepared from the cytoplasmic extract of BHK-21 cells infected with Newcastle disease virus (NDV). These membranes exhibited high hemagglutinating, neuraminidase, and hemolytic activity but little infectivity, suggesting that they might be precursors for viral envelope. When such membranes were adsorbed to the monolayers of uninfected BHK-21 cells at 4 degrees C and then incubated at elevated temperature for a couple of hours, the cells became highly hemadsorptive even in the presence of cycloheximide. This phenomenon occurred between 15 degrees C and 25 degrees C, and was maximal at 31 degrees C, where approximately 4 times more erythrocytes were adsorbed than to the cells incubated at 4 degrees C. Immunofluorescent staining suggested that diffusion of viral antigens might occurred rapidly over the entire surface of the cells. Cell fractions containing virions induced hemadsorption in uninfected cells, too. However, induction occurred now at 31 degrees C and was maximal at 37 degrees C, and erythrocytes appeared to be adsorbed not to the entire surface of the monolayer but restricted areas of the cells. The diffusion of viral antigens on the cell surface was not so significant under these conditions. On the basis of these findings the possible role of the membranes of the smooth endoplasmic reticulum in virus replication is discussed.", "contents": "Modification of normal cell surface by smooth membrane preparations from BHK-21 cells infected with Newcastle disease virus. Smooth membrane fractions were prepared from the cytoplasmic extract of BHK-21 cells infected with Newcastle disease virus (NDV). These membranes exhibited high hemagglutinating, neuraminidase, and hemolytic activity but little infectivity, suggesting that they might be precursors for viral envelope. When such membranes were adsorbed to the monolayers of uninfected BHK-21 cells at 4 degrees C and then incubated at elevated temperature for a couple of hours, the cells became highly hemadsorptive even in the presence of cycloheximide. This phenomenon occurred between 15 degrees C and 25 degrees C, and was maximal at 31 degrees C, where approximately 4 times more erythrocytes were adsorbed than to the cells incubated at 4 degrees C. Immunofluorescent staining suggested that diffusion of viral antigens might occurred rapidly over the entire surface of the cells. Cell fractions containing virions induced hemadsorption in uninfected cells, too. However, induction occurred now at 31 degrees C and was maximal at 37 degrees C, and erythrocytes appeared to be adsorbed not to the entire surface of the monolayer but restricted areas of the cells. The diffusion of viral antigens on the cell surface was not so significant under these conditions. On the basis of these findings the possible role of the membranes of the smooth endoplasmic reticulum in virus replication is discussed."}
{"id": "PMID:1101010", "title": "Re-evaluation of the Auxotab (Inolex) Enteric 1 System for identification of enterobacteriaceae.", "content": "The improved Auxotab Enteric I System was re-evaluated and found to correlate 98% with conventional methods for the identification of Enterobacteriaceae. Possible Reasons for previous poor results with this system are discussed.", "contents": "Re-evaluation of the Auxotab (Inolex) Enteric 1 System for identification of enterobacteriaceae. The improved Auxotab Enteric I System was re-evaluated and found to correlate 98% with conventional methods for the identification of Enterobacteriaceae. Possible Reasons for previous poor results with this system are discussed."}
{"id": "PMID:1101026", "title": "Conjugation in Escherichia coli: a study of recombination and the fate of donor DNA at the level of the zygote.", "content": "We have developed an experimental system for studying concomitantly the fate of the donor DNA and the process of recombination after conjugation in Escherichia coli. We used a set of Hfr and F-strains carrying complementing lacZ mutations. Expression of the lacZ allele on the chromosomal fragment derived from the donor results in the formation of heat sensitive beta-galactosidase by complementation. By intragenic recombination between the two lacZ mutations a lacZ+ gene may be formed, and wild type beta-galactosidase will be synthesized subsequently. So the assay of heat sensitive and wild type beta-galactosidase enabled us to follow respectively the fate of the donor DNA and the recombination process. Using various recombination deficient recipient strains, we found that the donor DNA is progressively inactivated in recA, rec-34 and recH recipients, although the initial rate of expression is equivalent to that in a Rec+ recipient; no significant recombination was observed. In Rec+, recB or recG recipients there was no inactivation and recombination occurred. The kinetics of recombinant formation in the recB strain seems to differ from the wild type; in a recG recipient the recombination activity is significant, but lower than in the wild type recipient.", "contents": "Conjugation in Escherichia coli: a study of recombination and the fate of donor DNA at the level of the zygote. We have developed an experimental system for studying concomitantly the fate of the donor DNA and the process of recombination after conjugation in Escherichia coli. We used a set of Hfr and F-strains carrying complementing lacZ mutations. Expression of the lacZ allele on the chromosomal fragment derived from the donor results in the formation of heat sensitive beta-galactosidase by complementation. By intragenic recombination between the two lacZ mutations a lacZ+ gene may be formed, and wild type beta-galactosidase will be synthesized subsequently. So the assay of heat sensitive and wild type beta-galactosidase enabled us to follow respectively the fate of the donor DNA and the recombination process. Using various recombination deficient recipient strains, we found that the donor DNA is progressively inactivated in recA, rec-34 and recH recipients, although the initial rate of expression is equivalent to that in a Rec+ recipient; no significant recombination was observed. In Rec+, recB or recG recipients there was no inactivation and recombination occurred. The kinetics of recombinant formation in the recB strain seems to differ from the wild type; in a recG recipient the recombination activity is significant, but lower than in the wild type recipient."}
{"id": "PMID:1101027", "title": "The regulatory nature of the phoB gene for alkaline phosphatase synthesis in Escherichia coli.", "content": "Quantitative measurements of alkaline phosphatase activity in various merozygotic combinations and electrophoretic analyses of periplasmic proteins derepressed by phosphate-starvation show that phoB is a positive regulatory gene.", "contents": "The regulatory nature of the phoB gene for alkaline phosphatase synthesis in Escherichia coli. Quantitative measurements of alkaline phosphatase activity in various merozygotic combinations and electrophoretic analyses of periplasmic proteins derepressed by phosphate-starvation show that phoB is a positive regulatory gene."}
{"id": "PMID:1101028", "title": "IS1 is involved in deletion formation in the gal region of E. coli K12.", "content": "The DNA sequence IS1, which is 800 pairs long, has been shown to integrate into various bacterial and phage operons. The presence of this DNA sequence in the gal operon of E. coli K12 leads to an 30-2000 fold increase in deletion formation in the gal region as compared to wildtype. This high frequency of deletion formation is specific for IS1 and is independent of the cellular recA function. While the frequency of reversion of gal::IS1 mutations, which also is independent of recA, is not affected by the growth temperature of the cells, the formation of deletions in the gal::IS1 system is strongly dependent on the temperature of growth. Mapping experiments showed that one endpoint of the deletions in most cases is at the site of the IS1 mutation and the second endpoint seems to be at various but preferred sites. The formation of the different classes of delections observed is affected differently by the growth temperature of the cells. A model to account for these results is presented.", "contents": "IS1 is involved in deletion formation in the gal region of E. coli K12. The DNA sequence IS1, which is 800 pairs long, has been shown to integrate into various bacterial and phage operons. The presence of this DNA sequence in the gal operon of E. coli K12 leads to an 30-2000 fold increase in deletion formation in the gal region as compared to wildtype. This high frequency of deletion formation is specific for IS1 and is independent of the cellular recA function. While the frequency of reversion of gal::IS1 mutations, which also is independent of recA, is not affected by the growth temperature of the cells, the formation of deletions in the gal::IS1 system is strongly dependent on the temperature of growth. Mapping experiments showed that one endpoint of the deletions in most cases is at the site of the IS1 mutation and the second endpoint seems to be at various but preferred sites. The formation of the different classes of delections observed is affected differently by the growth temperature of the cells. A model to account for these results is presented."}
{"id": "PMID:1101029", "title": "A new gene for ribosomal restriction in Escherichia coli.", "content": "Two mutations restricting the leakiness of an amber mutant are described. They were selected without the use of streptomycin: one maps in the strA region (at 64 minutes of the current E. coli chromosomal map) but is streptomycin sensitive and the other in the threonine region (at the origin of the map), 23% cotransducible with threonine by P1.", "contents": "A new gene for ribosomal restriction in Escherichia coli. Two mutations restricting the leakiness of an amber mutant are described. They were selected without the use of streptomycin: one maps in the strA region (at 64 minutes of the current E. coli chromosomal map) but is streptomycin sensitive and the other in the threonine region (at the origin of the map), 23% cotransducible with threonine by P1."}
{"id": "PMID:1101030", "title": "Tetracycline-sensitive mutants of the F-like R factors R100 and R100-1.", "content": "The majority of tetracycline-sensitive (Tets) mutants of R100 and R100-1 are multisite (deletion) mutants. About 50% of these are also transfer-deficient, indicating that the Tetr locus is closely linked to the transfer genes. Tet(s) mutants with single-site lesions are also described.", "contents": "Tetracycline-sensitive mutants of the F-like R factors R100 and R100-1. The majority of tetracycline-sensitive (Tets) mutants of R100 and R100-1 are multisite (deletion) mutants. About 50% of these are also transfer-deficient, indicating that the Tetr locus is closely linked to the transfer genes. Tet(s) mutants with single-site lesions are also described."}
{"id": "PMID:1101031", "title": "Antimutator activity during mitosis by a meiotic mutant of yeast.", "content": "Diploids homozygous for the mutation spo7-1 do not exhibit net premeiotic DNA synthesis at 34 degrees C and are defective in commitment to recombination following exposure to sporulation medium. The spo7-1 mutation confers antimutator activity during mitosis at 34 degrees C, indicating that the SPO7 gene product is involved in both mitotic and premeiotic DNA metabolism. Strains bearing spo7-1 are slightly more sensitive to killing by ultraviolet light than the wild type but are proficient in UV induced mutation and mitotic intragenic recombination. The mitotic antimutator activity of spo7-1 is directed against a class of forward mutations known to occur more frequently during mitosis than meiosis.", "contents": "Antimutator activity during mitosis by a meiotic mutant of yeast. Diploids homozygous for the mutation spo7-1 do not exhibit net premeiotic DNA synthesis at 34 degrees C and are defective in commitment to recombination following exposure to sporulation medium. The spo7-1 mutation confers antimutator activity during mitosis at 34 degrees C, indicating that the SPO7 gene product is involved in both mitotic and premeiotic DNA metabolism. Strains bearing spo7-1 are slightly more sensitive to killing by ultraviolet light than the wild type but are proficient in UV induced mutation and mitotic intragenic recombination. The mitotic antimutator activity of spo7-1 is directed against a class of forward mutations known to occur more frequently during mitosis than meiosis."}
{"id": "PMID:1101032", "title": "Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants.", "content": "In order to analyse how many structural genes are implicated in the specific steps of the biosynthesis of methionine in Sacch. cerevisiae, a hundred mutants were studied by complementation. 21 groups were defined named MET1 to MET25. Neither recombination between independent mutants of the same complementation group nor linkage between different groups was found. Preliminary to biochemical studies, mutants of each complementation group were tested for their capacity to utilize various precursors of methionine.", "contents": "Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. In order to analyse how many structural genes are implicated in the specific steps of the biosynthesis of methionine in Sacch. cerevisiae, a hundred mutants were studied by complementation. 21 groups were defined named MET1 to MET25. Neither recombination between independent mutants of the same complementation group nor linkage between different groups was found. Preliminary to biochemical studies, mutants of each complementation group were tested for their capacity to utilize various precursors of methionine."}
{"id": "PMID:1101033", "title": "A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12.", "content": "A temperature sensitive nonsense (TSN) mutant of E. coli K12 has been isolated in which a major bacterial protein is not synthesized at 42 degrees C. This protein is found in the parental strain at 42 degrees C and in cells rendered temperature resistant due to the insertion of a number of different nonsense suppressors or the normal allele of the mutant locus.", "contents": "A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12. A temperature sensitive nonsense (TSN) mutant of E. coli K12 has been isolated in which a major bacterial protein is not synthesized at 42 degrees C. This protein is found in the parental strain at 42 degrees C and in cells rendered temperature resistant due to the insertion of a number of different nonsense suppressors or the normal allele of the mutant locus."}
{"id": "PMID:1101034", "title": "Extensive segments of the Escherichia coli K12 chromosome in Proteus mirabilis diploids.", "content": "Various Escherichia coli K12 Hfr donors transfer at low frequency portions of the E. coli genome to Proteus mirabilis. By remating such Proteus hybrids with the same or a different E. coli Hfr strain, other genetic characters could be added to yield diploid Proteus hybrids which contained more than 30 percent of the E. coli genome. The extent of the E. coli genetic material in these unstable Proteus diploid hybrids included segments with the following selected markers: gal, lac, ara, mel, mtl, and malA. Unselected markers known to map throughout this region of the chromosome were also detected in these hybrids. Among the markers expressed in Proteus hybrids with the E. coli malA region was the receptor site for coliphage lambda. Although plaques were not seen, lambda was adsorbed by the Proteus hybrids. Examination of DNA from the various Proteus hybrids by CsCl density gradient centrifugation showed a satellite component of E. coli DNA with a size that corresponded to the extent of the E. coli genome present as determined by genetic analysis.", "contents": "Extensive segments of the Escherichia coli K12 chromosome in Proteus mirabilis diploids. Various Escherichia coli K12 Hfr donors transfer at low frequency portions of the E. coli genome to Proteus mirabilis. By remating such Proteus hybrids with the same or a different E. coli Hfr strain, other genetic characters could be added to yield diploid Proteus hybrids which contained more than 30 percent of the E. coli genome. The extent of the E. coli genetic material in these unstable Proteus diploid hybrids included segments with the following selected markers: gal, lac, ara, mel, mtl, and malA. Unselected markers known to map throughout this region of the chromosome were also detected in these hybrids. Among the markers expressed in Proteus hybrids with the E. coli malA region was the receptor site for coliphage lambda. Although plaques were not seen, lambda was adsorbed by the Proteus hybrids. Examination of DNA from the various Proteus hybrids by CsCl density gradient centrifugation showed a satellite component of E. coli DNA with a size that corresponded to the extent of the E. coli genome present as determined by genetic analysis."}
{"id": "PMID:1101036", "title": "Scintigraphy of the orbit with 67Ga-citrate.", "content": "The orbits of 50 patients with unilateral exophthalmos have been scanned with 67Ga-citrate. Of the 14 cases of malignant neoplasms in this series, only three cases did not show deposits in a characteristic manner. Some of pseudo-tumours showed patterns characteristic of malignant neoplasmas. 67Ga uptake of cavernous haemangioma was very low compared to the surrounding normal tissues.", "contents": "Scintigraphy of the orbit with 67Ga-citrate. The orbits of 50 patients with unilateral exophthalmos have been scanned with 67Ga-citrate. Of the 14 cases of malignant neoplasms in this series, only three cases did not show deposits in a characteristic manner. Some of pseudo-tumours showed patterns characteristic of malignant neoplasmas. 67Ga uptake of cavernous haemangioma was very low compared to the surrounding normal tissues."}
{"id": "PMID:1101041", "title": "Subtotal reconstruction of the orbit after destruction by benign tumour.", "content": "We present five cases of reconstruction of the orbital walls after destruction by tumours. Treatment is based upon wide exposure through a bicoronal scalp incision and immediate reconstruction with autogenous iliac bone grafts. we do not recommend using foreign materials for reconstructing the orbit.", "contents": "Subtotal reconstruction of the orbit after destruction by benign tumour. We present five cases of reconstruction of the orbital walls after destruction by tumours. Treatment is based upon wide exposure through a bicoronal scalp incision and immediate reconstruction with autogenous iliac bone grafts. we do not recommend using foreign materials for reconstructing the orbit."}
{"id": "PMID:1101043", "title": "Subtraction angiography of the orbit.", "content": "The principles and techniques of subtraction angiography are described. The superimposed confusing shadows are eliminated so that the small opacified arteries and veins as well as the choroidal plexus are clearly seen. The method is useful in the diagnosis of intraocular tumours and in localizing deeply seated intraocular foreign bodies.", "contents": "Subtraction angiography of the orbit. The principles and techniques of subtraction angiography are described. The superimposed confusing shadows are eliminated so that the small opacified arteries and veins as well as the choroidal plexus are clearly seen. The method is useful in the diagnosis of intraocular tumours and in localizing deeply seated intraocular foreign bodies."}
{"id": "PMID:1101045", "title": "Alcoholism: a pharmacogenetic disorder.", "content": "Alcoholism is an extremely common psychosocial behavioral disorder for which genetic factors may play an important role. Statistical analysis of special kinds of family studies that separate inherited factors from the common family environment point strongly to genetic predisposition. This paper presents data and speculations on the genetically determined differences among population subgroups and individuals in the acute effects of ethanol ingestion, the metabolism of ethanol, the process of tolerance, physical dependence and addictability, premorbid personality features and serious complications of alcoholism in the liver and brain of the alcoholic and in the offspring of alcoholic mothers.", "contents": "Alcoholism: a pharmacogenetic disorder. Alcoholism is an extremely common psychosocial behavioral disorder for which genetic factors may play an important role. Statistical analysis of special kinds of family studies that separate inherited factors from the common family environment point strongly to genetic predisposition. This paper presents data and speculations on the genetically determined differences among population subgroups and individuals in the acute effects of ethanol ingestion, the metabolism of ethanol, the process of tolerance, physical dependence and addictability, premorbid personality features and serious complications of alcoholism in the liver and brain of the alcoholic and in the offspring of alcoholic mothers."}
{"id": "PMID:1101046", "title": "Genetic factors and lithium response in manic-depressive illness.", "content": "The relationship between success in lithium treatment and the presence of affective illness in the families of manic-depressive patients was investigated in a double-blind study of lithium prophylaxis. Of a total of 89 outpatients who were followed for periods of up to 48 months, 43 were randomly assigned to lithium and 46 to placebo. 56% of the lithium-treated patients remained asymptomatic, as compared to 28% of the placebo patients. Of the 24 successfully treated lithium cases, 16 (66%) had at least one first-degree relative with bipolar illness, while only 4 of the 19 lithium failures (21%) had a first-degree relative with bipolar illness. No relationship was found between response to lithium and the presence of unipolar illness in the patients' families.", "contents": "Genetic factors and lithium response in manic-depressive illness. The relationship between success in lithium treatment and the presence of affective illness in the families of manic-depressive patients was investigated in a double-blind study of lithium prophylaxis. Of a total of 89 outpatients who were followed for periods of up to 48 months, 43 were randomly assigned to lithium and 46 to placebo. 56% of the lithium-treated patients remained asymptomatic, as compared to 28% of the placebo patients. Of the 24 successfully treated lithium cases, 16 (66%) had at least one first-degree relative with bipolar illness, while only 4 of the 19 lithium failures (21%) had a first-degree relative with bipolar illness. No relationship was found between response to lithium and the presence of unipolar illness in the patients' families."}
{"id": "PMID:1101048", "title": "Genetics of monoamine oxidase.", "content": "Evidence for the genetic control of human MAO is now well established. The relationships of MAO activities to neuropsychiatric disorders or response to psychopharmacologic treatments are relatively unstudied. Preliminary findings from our own studies suggest that (1) blood MAO activity is a polygenically controlled trait; (2) there may be a subgroup of depressed patients with high MAO activity who are more severe symptomatically and more resistant to treatment, and (3) that men with atypical and mixed depressions respond more favorably than women to treatment with an MAO inhibitory drug.", "contents": "Genetics of monoamine oxidase. Evidence for the genetic control of human MAO is now well established. The relationships of MAO activities to neuropsychiatric disorders or response to psychopharmacologic treatments are relatively unstudied. Preliminary findings from our own studies suggest that (1) blood MAO activity is a polygenically controlled trait; (2) there may be a subgroup of depressed patients with high MAO activity who are more severe symptomatically and more resistant to treatment, and (3) that men with atypical and mixed depressions respond more favorably than women to treatment with an MAO inhibitory drug."}
{"id": "PMID:1101049", "title": "Monoamine oxidase. Its inhibition.", "content": "Some 50 years ago the enzyme MAO was discovered by Hare and in the early 1930s Blaschko suggested that MAO may play an important role in the catabolism of monoamines in the central nervous system. With the discovery of iproniazid as an inhibitor of MAO and its introduction as an anti-depressant, many aspects of MAO activity and biogenic amine metabolism in experimental animals and man were examined. Although many other inhibitors of MAO were discovered and used therapeutically as anti-depressants, these drugs fell into disrepute largely because of their side-effects. Furthermore, their anti-depressant properties were questioned. After some years of relative inactivity there is now a revival of interest in the functional role of MAO in the central nervous system and drugs that inhibit or stimulate its activity \"specifically\". The basic reason for the upsurge of interest is that the enzyme from many tissues, including the brain of animals as well as man, has been purified and characterised. The evidence that neuronal MAO exist with different substrate and inhibitor specificities has led to the suggestion that they have physiological function and that deamination of non-methylated biogenic monoamines can take place in neurons. These findings have led to the advent of new drugs (clorgyline and depranil) with \"selective\" inhibition of enzyme forms. Their possible usage in the chemotherapy of depressive illness should be considered seriously. Fluctuation in peripheral organs as well as brain MAO is well documented. Recently they have been associated with changes in naturally occurring steroids. Although a decrease in platelet and brain MAO activity has been reported in a number of affect disorders (schizophrenia and bipolar depression) the results of these findings have recently been questioned (20, 141). Obviously further study in this area of research discussed is badly needed.", "contents": "Monoamine oxidase. Its inhibition. Some 50 years ago the enzyme MAO was discovered by Hare and in the early 1930s Blaschko suggested that MAO may play an important role in the catabolism of monoamines in the central nervous system. With the discovery of iproniazid as an inhibitor of MAO and its introduction as an anti-depressant, many aspects of MAO activity and biogenic amine metabolism in experimental animals and man were examined. Although many other inhibitors of MAO were discovered and used therapeutically as anti-depressants, these drugs fell into disrepute largely because of their side-effects. Furthermore, their anti-depressant properties were questioned. After some years of relative inactivity there is now a revival of interest in the functional role of MAO in the central nervous system and drugs that inhibit or stimulate its activity \"specifically\". The basic reason for the upsurge of interest is that the enzyme from many tissues, including the brain of animals as well as man, has been purified and characterised. The evidence that neuronal MAO exist with different substrate and inhibitor specificities has led to the suggestion that they have physiological function and that deamination of non-methylated biogenic monoamines can take place in neurons. These findings have led to the advent of new drugs (clorgyline and depranil) with \"selective\" inhibition of enzyme forms. Their possible usage in the chemotherapy of depressive illness should be considered seriously. Fluctuation in peripheral organs as well as brain MAO is well documented. Recently they have been associated with changes in naturally occurring steroids. Although a decrease in platelet and brain MAO activity has been reported in a number of affect disorders (schizophrenia and bipolar depression) the results of these findings have recently been questioned (20, 141). Obviously further study in this area of research discussed is badly needed."}
{"id": "PMID:1101052", "title": "Studies on the induction of mitotic gene conversion by ultraviolet irradiation. I. Analysis of dose-frequency relationship.", "content": "The UV (270-nm) dose-frequency relationship for the induction of intragenic mitotic recombination at trp 5 locus in Saccharomyces cerevisiae was non-linear. Two parameters, alpha and a, in the proposed equation for the non-linear relationship f = (at)alpha were determined so as to fit the experimental data by the method of least squares. The analysis was extended over 5 cell stages during synchronous growth. It was found that (1) parameter alpha changed from 2.02 for unbudded small cells to 1.09 for the stage where the cell had finished the division of the nucleus, and (2) parameter a changed correspondingly from 7.25-10(-4) to 0.180-10(-4) sec-1 during the same period. One interesting outcome in this analysis was the deduction of a dose-dependent nature of relative sensitivity with respect to the stage. The determination of these two parameters enabled us to calculate dose-effect relationships beyond the limits of experimental restrictions. Such an \"imaginary\" relationship, calculated at an extremely low dose, revealed the existence of maximal sensitivity around the DNA synthesis period. It was further shown that this maximum would easily be masked even in the moderate dose range. Thus, we conclude that the validity of single dose comparisons is diminished unless alpha is constant regardless of the cell stage. Some considerations on the proposed parameters have been made in relation to the mechanisms of the induction of gene conversion by UV.", "contents": "Studies on the induction of mitotic gene conversion by ultraviolet irradiation. I. Analysis of dose-frequency relationship. The UV (270-nm) dose-frequency relationship for the induction of intragenic mitotic recombination at trp 5 locus in Saccharomyces cerevisiae was non-linear. Two parameters, alpha and a, in the proposed equation for the non-linear relationship f = (at)alpha were determined so as to fit the experimental data by the method of least squares. The analysis was extended over 5 cell stages during synchronous growth. It was found that (1) parameter alpha changed from 2.02 for unbudded small cells to 1.09 for the stage where the cell had finished the division of the nucleus, and (2) parameter a changed correspondingly from 7.25-10(-4) to 0.180-10(-4) sec-1 during the same period. One interesting outcome in this analysis was the deduction of a dose-dependent nature of relative sensitivity with respect to the stage. The determination of these two parameters enabled us to calculate dose-effect relationships beyond the limits of experimental restrictions. Such an \"imaginary\" relationship, calculated at an extremely low dose, revealed the existence of maximal sensitivity around the DNA synthesis period. It was further shown that this maximum would easily be masked even in the moderate dose range. Thus, we conclude that the validity of single dose comparisons is diminished unless alpha is constant regardless of the cell stage. Some considerations on the proposed parameters have been made in relation to the mechanisms of the induction of gene conversion by UV."}
{"id": "PMID:1101053", "title": "Studies on the induction of mitotic gene conversion by ultraviolet irradiation. II. Action spectra.", "content": "Action spectra for the induction of intragenic mitotic recombination (gene conversion) at the trp 5 locus by UV are presented for three cell stages (T0, T9 and T16) taken from synchronously growing cultures of Saccharomyces cerevisiae. The spectra over the range from 230 to 300 nm were taken mostly in 5-nm steps. The peak of action spectra was significantly shifted, regardless of the stage, toward the longer wavelengths as compared with that of the absorption spectrum of DNA (258 nm) or even that of thymine (265 nm). In one extreme case (T16), the peak was shifted 17 nm from the absorption peak of DNA. Further, the spectrum changed its shape as the cell stage advanced from non-dividing (unbudded) (T0) to a dividing phase (T16). Furthermore, the induction cross section decreased by a large factor (about 40), regardless of the wavelength, in going from T0 to T16. From observations of the high photoreversibility of induced conversions, the major primary damage was thought to be pyrimidine dimers in the DNA. One plausible explanation, though not quite satisfactory from the quantitative viewpoint for these findings was that the increasing RNA during growth would screen the incident UV differentially with respect to the stage. If this explanation is correct, thymine dimers may still be considered, in spite of the shifts and deformations in the action spectra, as the major primary damage that triggers the long series of processes leading to gene conversion. Conventional methods for obtaining action spectra are discussed in comparison with the present method, which was based on sensitivity parameter a in the proposed dose (t)-frequency (f) relation, f = (at)alpha (alpha is the multiplicity parameter).", "contents": "Studies on the induction of mitotic gene conversion by ultraviolet irradiation. II. Action spectra. Action spectra for the induction of intragenic mitotic recombination (gene conversion) at the trp 5 locus by UV are presented for three cell stages (T0, T9 and T16) taken from synchronously growing cultures of Saccharomyces cerevisiae. The spectra over the range from 230 to 300 nm were taken mostly in 5-nm steps. The peak of action spectra was significantly shifted, regardless of the stage, toward the longer wavelengths as compared with that of the absorption spectrum of DNA (258 nm) or even that of thymine (265 nm). In one extreme case (T16), the peak was shifted 17 nm from the absorption peak of DNA. Further, the spectrum changed its shape as the cell stage advanced from non-dividing (unbudded) (T0) to a dividing phase (T16). Furthermore, the induction cross section decreased by a large factor (about 40), regardless of the wavelength, in going from T0 to T16. From observations of the high photoreversibility of induced conversions, the major primary damage was thought to be pyrimidine dimers in the DNA. One plausible explanation, though not quite satisfactory from the quantitative viewpoint for these findings was that the increasing RNA during growth would screen the incident UV differentially with respect to the stage. If this explanation is correct, thymine dimers may still be considered, in spite of the shifts and deformations in the action spectra, as the major primary damage that triggers the long series of processes leading to gene conversion. Conventional methods for obtaining action spectra are discussed in comparison with the present method, which was based on sensitivity parameter a in the proposed dose (t)-frequency (f) relation, f = (at)alpha (alpha is the multiplicity parameter)."}
{"id": "PMID:1101065", "title": "Hepatitis B immune globulin as a prophylactic measure for spouses exposed to acute type B hepatitis.", "content": "Because the value of usual immune globulin preparations in preventing Type B hepatitis is doubtful, we carried out a double-blind comparison of a control human immune globulin preparation with one--identified as HBIG--that had a high concentration (442 mug per milliliter) of antibodies to surface components of hepatitis B virus. Effectiveness was tested in spouses of patients with acute Type B hepatitis. Within 150 days after injection, nine of 33 spouses in the control group had symptomatic Type B hepatitis, compared with one of 25 spouses receiving HBIG. One non-B case also occurred in the HBIG group. Five control globulin recipients had evidence of subclinical hepatitis B infection, compared with one HBIG recipient.  Thus, HBIG appeared effective in suppressing not only disease, but also infection itself. Prophylactic value has been demonstrated in persons who should now be recognized as being at exceptionally high risk.", "contents": "Hepatitis B immune globulin as a prophylactic measure for spouses exposed to acute type B hepatitis. Because the value of usual immune globulin preparations in preventing Type B hepatitis is doubtful, we carried out a double-blind comparison of a control human immune globulin preparation with one--identified as HBIG--that had a high concentration (442 mug per milliliter) of antibodies to surface components of hepatitis B virus. Effectiveness was tested in spouses of patients with acute Type B hepatitis. Within 150 days after injection, nine of 33 spouses in the control group had symptomatic Type B hepatitis, compared with one of 25 spouses receiving HBIG. One non-B case also occurred in the HBIG group. Five control globulin recipients had evidence of subclinical hepatitis B infection, compared with one HBIG recipient.  Thus, HBIG appeared effective in suppressing not only disease, but also infection itself. Prophylactic value has been demonstrated in persons who should now be recognized as being at exceptionally high risk."}
{"id": "PMID:1101066", "title": "Hepatitis B \"immune\" globulin: effectiveness in prevention of dialysis-associated hepatitis.", "content": "A randomized, double-blind, multicenter study of hepatitis prevention by immune serum globulin with high anti-HBs titer (\"hepatitis B immune globulin\") was carried out among 318 new patients and 296 staff members of renal dialysis units. Three milliliters of high titer globulin, repeated at four months, was compared with equal doses of intermediate or normal titer globulin. Among staff members, the cumulative percentages developing hepatitis or HBs Ag, or both, within eight months were 6.9, 11.7, and 11.1 in the high, intermediate, and normal titer groups respectively. The lower incidence associated with high titer globulin was not significant (P greater than 0.05). However, among the patients the respective percentages were 7.9, 21.3, and 23.1 and the lower incidence in the high titer globulin group was significant.", "contents": "Hepatitis B \"immune\" globulin: effectiveness in prevention of dialysis-associated hepatitis. A randomized, double-blind, multicenter study of hepatitis prevention by immune serum globulin with high anti-HBs titer (\"hepatitis B immune globulin\") was carried out among 318 new patients and 296 staff members of renal dialysis units. Three milliliters of high titer globulin, repeated at four months, was compared with equal doses of intermediate or normal titer globulin. Among staff members, the cumulative percentages developing hepatitis or HBs Ag, or both, within eight months were 6.9, 11.7, and 11.1 in the high, intermediate, and normal titer groups respectively. The lower incidence associated with high titer globulin was not significant (P greater than 0.05). However, among the patients the respective percentages were 7.9, 21.3, and 23.1 and the lower incidence in the high titer globulin group was significant."}
{"id": "PMID:1101070", "title": "Multiple steps in DNA recognition by restriction endonuclease from E. coli K.", "content": "The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.", "contents": "Multiple steps in DNA recognition by restriction endonuclease from E. coli K. The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP."}
{"id": "PMID:1101072", "title": "Identification of two copies of the gene for the elongation factor EF-Tu in E. coli.", "content": "Two copies of the structural gene for the elongation factor EF-Tu have been identified in Escherichia coli: one near rif and the other near str. The latter seems to belong to a single transcriptional unit together with the genes for ribosomal protein S7, S12 (str) and the elongation factor EF-G (fus).", "contents": "Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Two copies of the structural gene for the elongation factor EF-Tu have been identified in Escherichia coli: one near rif and the other near str. The latter seems to belong to a single transcriptional unit together with the genes for ribosomal protein S7, S12 (str) and the elongation factor EF-G (fus)."}
{"id": "PMID:1101077", "title": "Morphine-induced running analgesia in two strains of mice following septal lesions or modification of brain amines.", "content": "Groups of two inbred strains of mice (C 57 B1/6J and DBA/2J) with septal and control lesions were tested for morphine-induced analgesia and running activity (running fit). As previously observed the effects of morphine on the running fit and analgesia were strain-dependent and a negative strain correlation was evident between the two measures in C 57 and DBA operated control mice which are characterized by different brain levels and turnover of cholinergic and adrenergic mediators. Septal lesions, which cause a reduction in the levels of acetylcholine in the brain areas which receive a cholinergikc input from the septum, anatogonized morphine analgesia in both strains while the running fit syndrome was unaffected. Pharmacological manipulation of brain catecholamines did not interfer with morphine-induced analgesia. The effacts of different pharmacological agents with interfere with noradrenaline synthesis and catecholamine oxidation were assessed in the two strains which are characterized by presence (C 57) or absence (DBA) of increased motor activity following the injection of morphine. The results argue against an exclusive association of morphine-induced motor activity with noradrenergic mechanism.", "contents": "Morphine-induced running analgesia in two strains of mice following septal lesions or modification of brain amines. Groups of two inbred strains of mice (C 57 B1/6J and DBA/2J) with septal and control lesions were tested for morphine-induced analgesia and running activity (running fit). As previously observed the effects of morphine on the running fit and analgesia were strain-dependent and a negative strain correlation was evident between the two measures in C 57 and DBA operated control mice which are characterized by different brain levels and turnover of cholinergic and adrenergic mediators. Septal lesions, which cause a reduction in the levels of acetylcholine in the brain areas which receive a cholinergikc input from the septum, anatogonized morphine analgesia in both strains while the running fit syndrome was unaffected. Pharmacological manipulation of brain catecholamines did not interfer with morphine-induced analgesia. The effacts of different pharmacological agents with interfere with noradrenaline synthesis and catecholamine oxidation were assessed in the two strains which are characterized by presence (C 57) or absence (DBA) of increased motor activity following the injection of morphine. The results argue against an exclusive association of morphine-induced motor activity with noradrenergic mechanism."}
{"id": "PMID:1101086", "title": "Relationship between the kidney and parathyroid hormone.", "content": "This report reviews the interrelationship between the activity of the parathyroid glands and renal function. Among the topics discussed are: effects of parathyroid hormone on various aspects of renal function such as: (1) glomerular filtration rate and renal blood flow; (2) renal handling of phosphorus, calcium, magnesium, sodium and potassium; (3) renal production of 1,25-dihydroxycholecalciferol; (4) renal handling of bicarbonate and acid-base metabolism, and (5) mechanism of action of parathyroid hormone on the renal cell. Further topics include: renal metabolism of parathyroid hormone; the kidney in hyperparathyroidism, and effects of renal failure on structure and function of the parathyroid glands.", "contents": "Relationship between the kidney and parathyroid hormone. This report reviews the interrelationship between the activity of the parathyroid glands and renal function. Among the topics discussed are: effects of parathyroid hormone on various aspects of renal function such as: (1) glomerular filtration rate and renal blood flow; (2) renal handling of phosphorus, calcium, magnesium, sodium and potassium; (3) renal production of 1,25-dihydroxycholecalciferol; (4) renal handling of bicarbonate and acid-base metabolism, and (5) mechanism of action of parathyroid hormone on the renal cell. Further topics include: renal metabolism of parathyroid hormone; the kidney in hyperparathyroidism, and effects of renal failure on structure and function of the parathyroid glands."}
{"id": "PMID:1101087", "title": "Thyroid hormone and the kidney.", "content": "Thyroid hormones affect both renal morphology and function. They are required for kidney growth and development, and thyroid deficiency results in decreased renal plasma flow and glomerular filtration rate and in impaired urinary concentration and dilution. Thyroid hormones also influence membrane transport and electrolyte metabolism, and alterations in mineral metabolism in hyperthyroidism frequently cause calcium nephropathy which affects renal function adversely. The kidney plays an important role in the peripheral metabolism of iodine and thyroid hormones, and thyroid function is altered in certain kidney diseases, particularly chronic renal failure. The pathogenesis of these alterations is currently under active investigation.", "contents": "Thyroid hormone and the kidney. Thyroid hormones affect both renal morphology and function. They are required for kidney growth and development, and thyroid deficiency results in decreased renal plasma flow and glomerular filtration rate and in impaired urinary concentration and dilution. Thyroid hormones also influence membrane transport and electrolyte metabolism, and alterations in mineral metabolism in hyperthyroidism frequently cause calcium nephropathy which affects renal function adversely. The kidney plays an important role in the peripheral metabolism of iodine and thyroid hormones, and thyroid function is altered in certain kidney diseases, particularly chronic renal failure. The pathogenesis of these alterations is currently under active investigation."}
{"id": "PMID:1101088", "title": "Kidney and adrenocortical hormones.", "content": "We have presented a review of the interrelationship between the kidney and the adrenocortical steroids, aldosterone and cortisol primarily in the regulation of water and electrolyte metabolism. The presentation is divided into three parts: (1) the influence of cortisol and aldosterone on renal structure and function; (2) the effect of kidney disease on secretion and metabolism of these steroids, and (3) the role of the kidneys in the plasma clearance of these steroids and their metabolites. There is no evidence that an excess or deficit of these steroids have a direct effect on renal structure, but both are necessary to maintain normal GFR and RPF. Glucocorticoids augment renal hemodynamics in pharmacologic doses. The phenomenon of 'escape' by the kidney from the sodium-retaining effect of adrenocortical steroids is discussed in detail, as well as the ability of glucocorticoid to antagonize the sodium retaining activity of any adrenal steroid of analogue with lesser glucocorticoid noperties. It is included that the impaired water dunesis of glycocorticoid deficiency is due to the absence of the permissive action of these steroids on the kidney, augmented at times by enhanced ADH secretion in response to sustained nonosmotic stimuli. The effect of gluco and mineralocorticoids on the renal excretion of divalentions and uric acid is also discussed. While progressive chronic renal failure (CRF) does not seem to significantly after the secretion or metabolism of cortisol it is possible that CRF causes a state of chronic hyperaldosteronism that is essential to maintain normal excretion (secretion) of potassium per nephron as renal mass progressively decreases. A direct or an indirect effect of potassium ion may be responsible for the hypersecretion or aldosterone rather than the renin-angiotensin system.", "contents": "Kidney and adrenocortical hormones. We have presented a review of the interrelationship between the kidney and the adrenocortical steroids, aldosterone and cortisol primarily in the regulation of water and electrolyte metabolism. The presentation is divided into three parts: (1) the influence of cortisol and aldosterone on renal structure and function; (2) the effect of kidney disease on secretion and metabolism of these steroids, and (3) the role of the kidneys in the plasma clearance of these steroids and their metabolites. There is no evidence that an excess or deficit of these steroids have a direct effect on renal structure, but both are necessary to maintain normal GFR and RPF. Glucocorticoids augment renal hemodynamics in pharmacologic doses. The phenomenon of 'escape' by the kidney from the sodium-retaining effect of adrenocortical steroids is discussed in detail, as well as the ability of glucocorticoid to antagonize the sodium retaining activity of any adrenal steroid of analogue with lesser glucocorticoid noperties. It is included that the impaired water dunesis of glycocorticoid deficiency is due to the absence of the permissive action of these steroids on the kidney, augmented at times by enhanced ADH secretion in response to sustained nonosmotic stimuli. The effect of gluco and mineralocorticoids on the renal excretion of divalentions and uric acid is also discussed. While progressive chronic renal failure (CRF) does not seem to significantly after the secretion or metabolism of cortisol it is possible that CRF causes a state of chronic hyperaldosteronism that is essential to maintain normal excretion (secretion) of potassium per nephron as renal mass progressively decreases. A direct or an indirect effect of potassium ion may be responsible for the hypersecretion or aldosterone rather than the renin-angiotensin system."}
{"id": "PMID:1101089", "title": "Renin and the kidney.", "content": "The factors involved in renin release have been extensively evaluated. The primary determinants are the transmural pressure at the afferent arteriole, sodium delivery to the macula densa, and the activity of the adrenergic nervous system. Other possible factors include circulating catecholamines, the serum and cerebrospinal fluid sodium concentration, serum potassium concentration, angiotensin II concentration, and antidiuretic hormone release. There is no convincing evidence that the renin-angiotensin system mediates renal autoregulation. Plasma renin activity is altered in a number of clinical settings. This parameter is elevated in most patients with cirrhosis and the nephrotic syndrome as well as in individuals with severe congestive heart failure. Despite inappropriately large weight gains, plasma renin suppresses normally with increased salt intake in edematous patients who have a normal glomerular filtration rate. The mechanisms of the alteration in the renin-angiotensin system in Bartter's syndrome is still not clear.", "contents": "Renin and the kidney. The factors involved in renin release have been extensively evaluated. The primary determinants are the transmural pressure at the afferent arteriole, sodium delivery to the macula densa, and the activity of the adrenergic nervous system. Other possible factors include circulating catecholamines, the serum and cerebrospinal fluid sodium concentration, serum potassium concentration, angiotensin II concentration, and antidiuretic hormone release. There is no convincing evidence that the renin-angiotensin system mediates renal autoregulation. Plasma renin activity is altered in a number of clinical settings. This parameter is elevated in most patients with cirrhosis and the nephrotic syndrome as well as in individuals with severe congestive heart failure. Despite inappropriately large weight gains, plasma renin suppresses normally with increased salt intake in edematous patients who have a normal glomerular filtration rate. The mechanisms of the alteration in the renin-angiotensin system in Bartter's syndrome is still not clear."}
{"id": "PMID:1101090", "title": "Insulin and the kidney.", "content": "Changes in renal function and structure are frequently observed in patients with diabetes mellitus. In the early phases of the disease, alterations in glomerular filtration rate, renal plasma flow, glomerular permeability and tubular capacity for glucose reabsorption occur. In the late stages of juvenile onset diabetes, renal failure is a common cause of death. For this reason, increasing attention is being paid to the possibility of long-term dialysis and renal transplantation in these patients. The kidneys play an important role in regulating insulin metabolism. The renal arteriovenous difference is approximately 30-45% and a linear relationship exists between the arterial insulin level and the renal arteriovenous concentration difference. The renal extraction of insulin is 200 ml/min in man, and it is estimated that 6-8 U are removed and degraded by the kidney in 24 h. The quantity of insulin in urine is small. However, its clearance is relatively constant over a wide range of serum concentrations and is 0.15-0.5 ml/min. The mean basal insulin excretion is 3.6 muU/mg creatinine, and a fourfold rise occurs following a glucose load. The urinary insulin values in neonates, children and patients with diabetes and renal failure are reviewed. In diabetic patients, progressive renal disease is accompanied by decreasing insulin requirements. In contrast, nondiabetic patients who develop renal failure frequently show abnormalities in carbohydrate metabolism, the commonest of which is a pseudodiabetic state.", "contents": "Insulin and the kidney. Changes in renal function and structure are frequently observed in patients with diabetes mellitus. In the early phases of the disease, alterations in glomerular filtration rate, renal plasma flow, glomerular permeability and tubular capacity for glucose reabsorption occur. In the late stages of juvenile onset diabetes, renal failure is a common cause of death. For this reason, increasing attention is being paid to the possibility of long-term dialysis and renal transplantation in these patients. The kidneys play an important role in regulating insulin metabolism. The renal arteriovenous difference is approximately 30-45% and a linear relationship exists between the arterial insulin level and the renal arteriovenous concentration difference. The renal extraction of insulin is 200 ml/min in man, and it is estimated that 6-8 U are removed and degraded by the kidney in 24 h. The quantity of insulin in urine is small. However, its clearance is relatively constant over a wide range of serum concentrations and is 0.15-0.5 ml/min. The mean basal insulin excretion is 3.6 muU/mg creatinine, and a fourfold rise occurs following a glucose load. The urinary insulin values in neonates, children and patients with diabetes and renal failure are reviewed. In diabetic patients, progressive renal disease is accompanied by decreasing insulin requirements. In contrast, nondiabetic patients who develop renal failure frequently show abnormalities in carbohydrate metabolism, the commonest of which is a pseudodiabetic state."}
{"id": "PMID:1101091", "title": "Erythropoietin and the kidney.", "content": "Erythropoietin is a polypeptide hormone, which is produced in response to a deficit in the delivery of oxygen to the tissues relative to their oxygen needs; although other feedback mechanisms also modulate its production. The kidneys play a major role in the elaboration of this hormone. However, the precise action of the kidney is not yet known. Diseases of the kidneys cause profound alteration in the normal regulation of erythropoiesis. Some of these are reviewed here.", "contents": "Erythropoietin and the kidney. Erythropoietin is a polypeptide hormone, which is produced in response to a deficit in the delivery of oxygen to the tissues relative to their oxygen needs; although other feedback mechanisms also modulate its production. The kidneys play a major role in the elaboration of this hormone. However, the precise action of the kidney is not yet known. Diseases of the kidneys cause profound alteration in the normal regulation of erythropoiesis. Some of these are reviewed here."}
{"id": "PMID:1101092", "title": "Renal prostaglandins.", "content": "The renal prostaglandins PGS2 and PGE2 possess potent antihypertensive and vasodepressor activity. The mechanism of blood pressure lowering effect is through peripheral arteriolar dilation with a fall in total peripheral resistance. PGA unlike PGE escape degradation by the lung and thus could circulate as antihypertensive hormones. Since plasma PGA levels rise in humans on a low sodium intake, it has been postulated that the beneficial effects of a low sodium diet in some hypertensives may be the result of an increase in peripheral vasodilating PGA. Support that plasma PGA may be a regulator of systemic blood pressure is also derived from the fact a PGA-secreting renal tumor was associated with a fall in blood pressure and a rise in plasma PGA in a previously hypertensive woman. The removal of the tumor resulted in a return of blood pressure to elevated levels and a concomitant fall in PGA. Recently, a number of human patients with essential hypertension have been infused with PGA1 and PGA2. It was observed that there was an initial increase in renal blood flow, sodium and water excretion which was associated with no change in the elevated blood pressure. When blood pressure ultimately fell, there was a return of renal blood flow, sodium and water excretion to preinfusion levels. It would appear that PGA compounds act as 'ideal' antihypertensive agents since they favorably effect renal resistance, sodium and water homeostasis, plasma volume, total peripheral resistance, blood pressure and indirectly cardiac output through baroreceptor stimulation, all factors known to be important in etiology in human hypertension.", "contents": "Renal prostaglandins. The renal prostaglandins PGS2 and PGE2 possess potent antihypertensive and vasodepressor activity. The mechanism of blood pressure lowering effect is through peripheral arteriolar dilation with a fall in total peripheral resistance. PGA unlike PGE escape degradation by the lung and thus could circulate as antihypertensive hormones. Since plasma PGA levels rise in humans on a low sodium intake, it has been postulated that the beneficial effects of a low sodium diet in some hypertensives may be the result of an increase in peripheral vasodilating PGA. Support that plasma PGA may be a regulator of systemic blood pressure is also derived from the fact a PGA-secreting renal tumor was associated with a fall in blood pressure and a rise in plasma PGA in a previously hypertensive woman. The removal of the tumor resulted in a return of blood pressure to elevated levels and a concomitant fall in PGA. Recently, a number of human patients with essential hypertension have been infused with PGA1 and PGA2. It was observed that there was an initial increase in renal blood flow, sodium and water excretion which was associated with no change in the elevated blood pressure. When blood pressure ultimately fell, there was a return of renal blood flow, sodium and water excretion to preinfusion levels. It would appear that PGA compounds act as 'ideal' antihypertensive agents since they favorably effect renal resistance, sodium and water homeostasis, plasma volume, total peripheral resistance, blood pressure and indirectly cardiac output through baroreceptor stimulation, all factors known to be important in etiology in human hypertension."}
{"id": "PMID:1101093", "title": "Natriuretic hormone.", "content": "The factors that control sodium excretion have been the subject of considerable work in recent years. The natriuresis that follows expansion of the extracellular fluid volume is mediated to a large extent by inhibition of tubular sodium reabsorption. Of the several mechanisms proposed to explain this inhibition, considerable interest has followed the suggestion that a humoral substance is released into the blood stream in response to volume expansion. The present article reviews the evidence for and against the existence of such a humoral substance (natriuretic hormone). It also discusses its proposed chemical nature and its possible site of origin in the organism as well as its mode of action.", "contents": "Natriuretic hormone. The factors that control sodium excretion have been the subject of considerable work in recent years. The natriuresis that follows expansion of the extracellular fluid volume is mediated to a large extent by inhibition of tubular sodium reabsorption. Of the several mechanisms proposed to explain this inhibition, considerable interest has followed the suggestion that a humoral substance is released into the blood stream in response to volume expansion. The present article reviews the evidence for and against the existence of such a humoral substance (natriuretic hormone). It also discusses its proposed chemical nature and its possible site of origin in the organism as well as its mode of action."}
{"id": "PMID:1101094", "title": "Intention myoclonus of multiple sclerosis, its patho-anatomical basis and its stereotactic relief.", "content": "The typical multiple sclerosis case considered here is especially informative from both the standpoint of its clinical course and on the basis of the autopsy findings. The foci responsible for the severe bilateral intention myoclonus of the trunk and limbs are the nerve cell losses in both red nuclei due to extensive and almost complete demyelination. Thereby the triangle of Mollaret between the red nucleus, inferior olives and dentate nucleus is involved as the patho-physiological circuit responsible for myoclonus. Stereotactic coagulation of dentato-thalamic fibres resulted in complete relief of intention myoclonus. With regard to the triggering of fresh demyelinating foci by stereotactic interventions, our point of view is as follows: Although a stereotactic operation introduces the possibility of triggering new demyelinating foci in less than 10% of the cases, such a possibility does not represent an absolute contra-indication to the stereotactic treatment of action myoclonus in multiple sclerosis, if the patient is informed accordingly.", "contents": "Intention myoclonus of multiple sclerosis, its patho-anatomical basis and its stereotactic relief. The typical multiple sclerosis case considered here is especially informative from both the standpoint of its clinical course and on the basis of the autopsy findings. The foci responsible for the severe bilateral intention myoclonus of the trunk and limbs are the nerve cell losses in both red nuclei due to extensive and almost complete demyelination. Thereby the triangle of Mollaret between the red nucleus, inferior olives and dentate nucleus is involved as the patho-physiological circuit responsible for myoclonus. Stereotactic coagulation of dentato-thalamic fibres resulted in complete relief of intention myoclonus. With regard to the triggering of fresh demyelinating foci by stereotactic interventions, our point of view is as follows: Although a stereotactic operation introduces the possibility of triggering new demyelinating foci in less than 10% of the cases, such a possibility does not represent an absolute contra-indication to the stereotactic treatment of action myoclonus in multiple sclerosis, if the patient is informed accordingly."}
{"id": "PMID:1101096", "title": "Separation of brain-RNA by micro-electrophoresis on agarose-acrylamide gels.", "content": "A simple method is described for RNA fractionation on the microscale (10(-8) to 10(-7) g). The optimal conditions for electrophoretic separation of low molecular weight RNA (4S-16S) on such a scale have been determined running a standard mixture of 4S, 5S and 16S E. coli RNAs on agarose-polyacrylamide gels at three different concentrations of acrylamide. The separations were evaluated for resolution of the different species and for linearity in the relationship between mobility and logarithm of molecular weight. A straight linear relationship and good resolution was obtained on a 3.48 per cent polyacrylamide - 0.7 per cent agarose gel. When a sample of 10(-2) O.D.U.260 of macroextracted cerebral RNA was separated on such a gel, six unusual RNA bands were detected between 5A and 18S. To find out optimal conditions for RNA fractionation in the 16S-28S range a standard mixture of 16S, 18S, 23S and 28S RNA was run on gels of three different acrylamide concentrations. The separations were again evaluated with regard to both the resolution and the linearity of the relationship between the logarithms of the molecular weights and the mobilities of the standard RNA species. A 0.7 per cent agarose - 2.5 per cent polyacrylamide gel appeared to be quite suitable with regard to both. The fractionation of a microsample of RNA macroextracted from whole rat brain is presented. We also present the fractionation of the RNA microextracted from 1 mg of the CA3 region of rat hippocampus.", "contents": "Separation of brain-RNA by micro-electrophoresis on agarose-acrylamide gels. A simple method is described for RNA fractionation on the microscale (10(-8) to 10(-7) g). The optimal conditions for electrophoretic separation of low molecular weight RNA (4S-16S) on such a scale have been determined running a standard mixture of 4S, 5S and 16S E. coli RNAs on agarose-polyacrylamide gels at three different concentrations of acrylamide. The separations were evaluated for resolution of the different species and for linearity in the relationship between mobility and logarithm of molecular weight. A straight linear relationship and good resolution was obtained on a 3.48 per cent polyacrylamide - 0.7 per cent agarose gel. When a sample of 10(-2) O.D.U.260 of macroextracted cerebral RNA was separated on such a gel, six unusual RNA bands were detected between 5A and 18S. To find out optimal conditions for RNA fractionation in the 16S-28S range a standard mixture of 16S, 18S, 23S and 28S RNA was run on gels of three different acrylamide concentrations. The separations were again evaluated with regard to both the resolution and the linearity of the relationship between the logarithms of the molecular weights and the mobilities of the standard RNA species. A 0.7 per cent agarose - 2.5 per cent polyacrylamide gel appeared to be quite suitable with regard to both. The fractionation of a microsample of RNA macroextracted from whole rat brain is presented. We also present the fractionation of the RNA microextracted from 1 mg of the CA3 region of rat hippocampus."}
{"id": "PMID:1101099", "title": "Comparison of dopa decarboxylase inhibitor (carbidopa) combined with levodopa and levodopa alone in Parkinson's disease.", "content": "A double-blind study comparing the effects of carbidopa and levodopa combined in a single tablet with levodopa alone was undertaken in 50 patients with Parkinson's disease. After 6 months, there was a statistically significant improvement over baseline in total score, rigidity, and tremor only in the patients randomized to carbidopa/levodopa. In addition, 40 percent of the patients treated with carbidopa/levodopa showed obvious clinical improvement (a greater than 50 percent reduction in their total score) over treatment with levodopa alone. However, after 2 years, only 20 percent continued to show this improvement. Nausea, vomiting, and anorexia developed in 56 percent of patients on levodopa but in only 27 percent of patients on carbidopa/levodopa. However, abnormal involuntary movements, observed in 48 percent of patients on levodopa, were present in 77 percent of patients on carbidopa/levodopa. Despite the increase in abnormal involuntary movements, carbidopa/levodopa is more effective than levodopa.", "contents": "Comparison of dopa decarboxylase inhibitor (carbidopa) combined with levodopa and levodopa alone in Parkinson's disease. A double-blind study comparing the effects of carbidopa and levodopa combined in a single tablet with levodopa alone was undertaken in 50 patients with Parkinson's disease. After 6 months, there was a statistically significant improvement over baseline in total score, rigidity, and tremor only in the patients randomized to carbidopa/levodopa. In addition, 40 percent of the patients treated with carbidopa/levodopa showed obvious clinical improvement (a greater than 50 percent reduction in their total score) over treatment with levodopa alone. However, after 2 years, only 20 percent continued to show this improvement. Nausea, vomiting, and anorexia developed in 56 percent of patients on levodopa but in only 27 percent of patients on carbidopa/levodopa. However, abnormal involuntary movements, observed in 48 percent of patients on levodopa, were present in 77 percent of patients on carbidopa/levodopa. Despite the increase in abnormal involuntary movements, carbidopa/levodopa is more effective than levodopa."}
{"id": "PMID:1101097", "title": "[T-rosette test in Wilson's disease].", "content": "Twenty-three patients with Wilson's disease were studied. Using the rosette formation test it was found that the count of T-lymphocytes in the peripheral blood of these patients (29,77% +/- 12,58) was lower than in the control group (61,68% +/- 13,14). Using skin tests for demonstration of delayed hypersensitivity it was noted that in the group of patients the frequency of positive reaction with Candida albicans antigen was lower and these patients showed also less frequently positive reactions after DNCB immunization. The obtained results indicate that in Wilson's disease the functions of T-lymphocytes are distrubed. This may be the cause of previously observed hyperactivity of B-lymphocytes.", "contents": "[T-rosette test in Wilson's disease]. Twenty-three patients with Wilson's disease were studied. Using the rosette formation test it was found that the count of T-lymphocytes in the peripheral blood of these patients (29,77% +/- 12,58) was lower than in the control group (61,68% +/- 13,14). Using skin tests for demonstration of delayed hypersensitivity it was noted that in the group of patients the frequency of positive reaction with Candida albicans antigen was lower and these patients showed also less frequently positive reactions after DNCB immunization. The obtained results indicate that in Wilson's disease the functions of T-lymphocytes are distrubed. This may be the cause of previously observed hyperactivity of B-lymphocytes."}
{"id": "PMID:1101106", "title": "[Antiperistaltic esophago-jejuno-duodenostomy after total gastrectomy].", "content": "Total gastrectomy forms an essential part of surgical management of gastric carcinoma. The various operations proposed for reconstruction of the alimentary tract are reviewed, reflux oesophagitis, dehiscence of suture. Lastly, the advantages offered by oesophago-jejuno-duodenostomy are illustrated.", "contents": "[Antiperistaltic esophago-jejuno-duodenostomy after total gastrectomy]. Total gastrectomy forms an essential part of surgical management of gastric carcinoma. The various operations proposed for reconstruction of the alimentary tract are reviewed, reflux oesophagitis, dehiscence of suture. Lastly, the advantages offered by oesophago-jejuno-duodenostomy are illustrated."}
{"id": "PMID:1101107", "title": "[Clinical experiences with the use of a new antibiotic in inflammatory diseases of surgical interest].", "content": "Therapeutic and prophylactic management of 23 adults with inflammation of surgical interest using a new antibiotic, cefalexin sodium (Glaxo). 1.5-6 g/day were administered either i.m. (2 cases) or i.v. (21 cases) for 4 to 13 days. Examinations carried out before and after treatment showed that no damage was caused to the liver, kidney or haematopoietic apparatus. The clinical pharmacokinetic data made it clear that high serum levels were reached and renal elimination of the drug was almost complete. Cepoven displayed a high therapeutic capacity in nearly all cases. Both general and local tolerance were excellent.", "contents": "[Clinical experiences with the use of a new antibiotic in inflammatory diseases of surgical interest]. Therapeutic and prophylactic management of 23 adults with inflammation of surgical interest using a new antibiotic, cefalexin sodium (Glaxo). 1.5-6 g/day were administered either i.m. (2 cases) or i.v. (21 cases) for 4 to 13 days. Examinations carried out before and after treatment showed that no damage was caused to the liver, kidney or haematopoietic apparatus. The clinical pharmacokinetic data made it clear that high serum levels were reached and renal elimination of the drug was almost complete. Cepoven displayed a high therapeutic capacity in nearly all cases. Both general and local tolerance were excellent."}
{"id": "PMID:1101112", "title": "Antibiotic sensitivity of pathogens from infected skin lesions.", "content": "One hundred and seven patients attending an accident and emergency department with infected skin lesions were studied. The commonest organisms isolated were Staph aureus and Strep pyrogenes. Routine antimicrobial sensitivity testing of bacterial isolates showed that the antibiotic most likely to be effective against the organisms isolated was a cephalosporin or a tetracycline.", "contents": "Antibiotic sensitivity of pathogens from infected skin lesions. One hundred and seven patients attending an accident and emergency department with infected skin lesions were studied. The commonest organisms isolated were Staph aureus and Strep pyrogenes. Routine antimicrobial sensitivity testing of bacterial isolates showed that the antibiotic most likely to be effective against the organisms isolated was a cephalosporin or a tetracycline."}
{"id": "PMID:1101121", "title": "The Gambee intestinal anastomosis in gynecologic surgery.", "content": "The Gambee single-layer, through-and-through, catgut suture anastomosis of the gastrointestinal tract has been in use from 1969 to 1974. Sixty-four anastomoses have been performed on 56 patients with gastrointestinal involvement or pelvic malignancy. There were no operative deaths, and no patients required reoperation for correction of intestinal anastomotic leaks or rupture of the suture line. This experience suggests that the Gambee intestinal single-layer anastomosis may be useful in the management of gastrointestinal complications of pelvic surgery and malignancy.", "contents": "The Gambee intestinal anastomosis in gynecologic surgery. The Gambee single-layer, through-and-through, catgut suture anastomosis of the gastrointestinal tract has been in use from 1969 to 1974. Sixty-four anastomoses have been performed on 56 patients with gastrointestinal involvement or pelvic malignancy. There were no operative deaths, and no patients required reoperation for correction of intestinal anastomotic leaks or rupture of the suture line. This experience suggests that the Gambee intestinal single-layer anastomosis may be useful in the management of gastrointestinal complications of pelvic surgery and malignancy."}
{"id": "PMID:1101122", "title": "Follicle-stimulating hormone response to luteinizing-hormone-releasing hormone in patients with hydatidiform mole.", "content": "The serum concentration of immunoreactive follicle-stimulating hormone (FSH) and FSH response to synthetic luteinizing-hormone-releasing hormone (LHRH) were investigated in 9 patients with intact hydatidiform moles and 3 patients with aborted moles. The serum concentration of FSH before and after intravenous administration of 200 mug of synthetic LHRH was measured by double antibody radioimmunoassay. Serum FSH was detectable but relatively low compared with the levels seen in the follicular phase of the normal menstrual cycle in all 12 patients. Furthermore, the 12 patients did not respond, or responded subnormally, to exogenously administered LHRH, and there was no significant elevation in serum FSH levels after LHRH injection. The result of the current study is in accord with that of our previous report on the serum concentration of FSH and FSH response to synthetic LHRH during normal pregnancy. Thus, it might be concluded that the follicle-stimulating activity of the anterior pituitary is suppressed by an unknown mechanism during molar pregnancy as well as normal pregnancy.", "contents": "Follicle-stimulating hormone response to luteinizing-hormone-releasing hormone in patients with hydatidiform mole. The serum concentration of immunoreactive follicle-stimulating hormone (FSH) and FSH response to synthetic luteinizing-hormone-releasing hormone (LHRH) were investigated in 9 patients with intact hydatidiform moles and 3 patients with aborted moles. The serum concentration of FSH before and after intravenous administration of 200 mug of synthetic LHRH was measured by double antibody radioimmunoassay. Serum FSH was detectable but relatively low compared with the levels seen in the follicular phase of the normal menstrual cycle in all 12 patients. Furthermore, the 12 patients did not respond, or responded subnormally, to exogenously administered LHRH, and there was no significant elevation in serum FSH levels after LHRH injection. The result of the current study is in accord with that of our previous report on the serum concentration of FSH and FSH response to synthetic LHRH during normal pregnancy. Thus, it might be concluded that the follicle-stimulating activity of the anterior pituitary is suppressed by an unknown mechanism during molar pregnancy as well as normal pregnancy."}
{"id": "PMID:1101127", "title": "Upper lid loading with dermis graft and levator weakening. Management of lagophthalmos due to facial palsy.", "content": "A method to protect the cornea in case of lagophthalmos is presented, consisting in weighting the upper eyelid with dermis graft, combined with a weakening of the levator palpebrae. The technique is described, and the method is discussed versus other methods of upper lid loading.", "contents": "Upper lid loading with dermis graft and levator weakening. Management of lagophthalmos due to facial palsy. A method to protect the cornea in case of lagophthalmos is presented, consisting in weighting the upper eyelid with dermis graft, combined with a weakening of the levator palpebrae. The technique is described, and the method is discussed versus other methods of upper lid loading."}
{"id": "PMID:1101128", "title": "Diagnostic importance of immunofluorescence in oral bullous diseases and lupus erythematosus.", "content": "Immunofluorescent tests have proved to be of diagnostic importance for pemphigus, bullous pemphigoid, cicatricial pemphigoid, systemic lupus erythematosus, and discoid lupus erythematosus. Immunofluorescence test procedures, necessary specimens, and test findings have been reviewed as aids to dentists in the utilization and interpretation of these tests for the study of oral lesions.", "contents": "Diagnostic importance of immunofluorescence in oral bullous diseases and lupus erythematosus. Immunofluorescent tests have proved to be of diagnostic importance for pemphigus, bullous pemphigoid, cicatricial pemphigoid, systemic lupus erythematosus, and discoid lupus erythematosus. Immunofluorescence test procedures, necessary specimens, and test findings have been reviewed as aids to dentists in the utilization and interpretation of these tests for the study of oral lesions."}
{"id": "PMID:1101129", "title": "Continued anticoagulation in oral surgery procedures.", "content": "Anticoagulants are used in a number of clinical settings for the prevention and treatment of thromboembolic disease. The oral surgeon is seeing an increasing number of these anticoagulated patients with oral and dental disease. A technique is presented which minimizes the nonanticoagulated time for a patient requiring an oral surgical procedure. The technique requires hospitalization and should be used only for patients who are in danger of thromboembolism.", "contents": "Continued anticoagulation in oral surgery procedures. Anticoagulants are used in a number of clinical settings for the prevention and treatment of thromboembolic disease. The oral surgeon is seeing an increasing number of these anticoagulated patients with oral and dental disease. A technique is presented which minimizes the nonanticoagulated time for a patient requiring an oral surgical procedure. The technique requires hospitalization and should be used only for patients who are in danger of thromboembolism."}
{"id": "PMID:1101130", "title": "Clinical evaluation of the (+) isomer of mepivacaine.", "content": "A human clinical study was undertaken to evaluate the effectiveness and safety of dexivacaine 2 per cent, mepivacaine 2 per cent, and mepivacaine 3 per cent. A double-blind technique was used to evaluate time of onset, duration, depth of anesthesia, and side effects. Results indicated no significant differences among the three anesthetics, except that mepivacaine 3 per cent had a significantly longer duration of soft-tissue symptoms in the mandibular block series. The few side effects noted were minimal and of no statistical significance. The evaluation of an anesthetic by the several parameters employed and especially by the over-all opinion of the anesthesia was deemed a valuable research method whem comparing the clinical effectiveness of local anesthetics.", "contents": "Clinical evaluation of the (+) isomer of mepivacaine. A human clinical study was undertaken to evaluate the effectiveness and safety of dexivacaine 2 per cent, mepivacaine 2 per cent, and mepivacaine 3 per cent. A double-blind technique was used to evaluate time of onset, duration, depth of anesthesia, and side effects. Results indicated no significant differences among the three anesthetics, except that mepivacaine 3 per cent had a significantly longer duration of soft-tissue symptoms in the mandibular block series. The few side effects noted were minimal and of no statistical significance. The evaluation of an anesthetic by the several parameters employed and especially by the over-all opinion of the anesthesia was deemed a valuable research method whem comparing the clinical effectiveness of local anesthetics."}
{"id": "PMID:1101132", "title": "The microbiology of musculoskeletal infection.", "content": "Of paramount importance in the isolation and identification of bacteria, including mycobacteria, and fungi that cause musculoskeletal infections, are proper selection, collection, and transport of specimens. Material obtained by biopsy, curettage, or aspiration is preferable to that obtained on a swab because too little or unrepresentative material is usually collected on a swab and because swabs cannot be transported readily under conditions favorable to survival of anaerobes. Although actual infections are frequently due to staphylococci, strepococci, Enterobacteriaceae, and Pseudomonadacease, the etiologic agents of chronic lesions may include actinomycetes, brucellae, mycobacteria, and fungi. In chronic lesions, histopathologic examination of biopsy material is an important ancillary procedure. In acute infections, the incidence of gram-negative bacilli, particularly of Enterobacteriaceae and pseudomonads, has increased significantly in recent years.", "contents": "The microbiology of musculoskeletal infection. Of paramount importance in the isolation and identification of bacteria, including mycobacteria, and fungi that cause musculoskeletal infections, are proper selection, collection, and transport of specimens. Material obtained by biopsy, curettage, or aspiration is preferable to that obtained on a swab because too little or unrepresentative material is usually collected on a swab and because swabs cannot be transported readily under conditions favorable to survival of anaerobes. Although actual infections are frequently due to staphylococci, strepococci, Enterobacteriaceae, and Pseudomonadacease, the etiologic agents of chronic lesions may include actinomycetes, brucellae, mycobacteria, and fungi. In chronic lesions, histopathologic examination of biopsy material is an important ancillary procedure. In acute infections, the incidence of gram-negative bacilli, particularly of Enterobacteriaceae and pseudomonads, has increased significantly in recent years."}
{"id": "PMID:1101133", "title": "Septic arthritis in children.", "content": "When septic arthritis involves the hip, the prognosis is much worse than with any other joint. Approximately 50 per cent of the results were unsatisfactory with hip involvement compared to 12 per cent unsatisfactory results with involvement of the other joints. The erythrocyte sedimentation rate is a much more helpful diagnostic test than the leukocyte count or differential cell count. The most important prognostic factors are the duration of symptoms prior to treatment, a penicillinase-producing organism (penicillin-resistant) as the etiologic agent, and evidence of associated metaphyseal osteomyelitis. Early decompression and cleansing of the joint by aspiration or arthrotomy are essential for a good result. Long-term parenteral antibiotic therapy improves the prognosis when osteomyelitis is an assoicated feature. Arthrotomy with continuous irrigation appears to be more effective in decreasing long-term residual effects than arthrotomy alone. Almost all secondary surgical procedures are directed toward correcting the sequelae of septic arthritis of the hip.", "contents": "Septic arthritis in children. When septic arthritis involves the hip, the prognosis is much worse than with any other joint. Approximately 50 per cent of the results were unsatisfactory with hip involvement compared to 12 per cent unsatisfactory results with involvement of the other joints. The erythrocyte sedimentation rate is a much more helpful diagnostic test than the leukocyte count or differential cell count. The most important prognostic factors are the duration of symptoms prior to treatment, a penicillinase-producing organism (penicillin-resistant) as the etiologic agent, and evidence of associated metaphyseal osteomyelitis. Early decompression and cleansing of the joint by aspiration or arthrotomy are essential for a good result. Long-term parenteral antibiotic therapy improves the prognosis when osteomyelitis is an assoicated feature. Arthrotomy with continuous irrigation appears to be more effective in decreasing long-term residual effects than arthrotomy alone. Almost all secondary surgical procedures are directed toward correcting the sequelae of septic arthritis of the hip."}
{"id": "PMID:1101148", "title": "Surgery of the nasal tip.", "content": "Three techniques of nasal tip cartilage modifications are discussed in detail: the delivery, retroversion, and split (intracartilaginous) techniques. Ancillary procedures of the nasal tip, such as septoplasty without decreasing support, secondary surgery and post-traumatic nasal tip problems, and the rationale for various procedures are discussed.", "contents": "Surgery of the nasal tip. Three techniques of nasal tip cartilage modifications are discussed in detail: the delivery, retroversion, and split (intracartilaginous) techniques. Ancillary procedures of the nasal tip, such as septoplasty without decreasing support, secondary surgery and post-traumatic nasal tip problems, and the rationale for various procedures are discussed."}
{"id": "PMID:1101149", "title": "Augmentation rhinoplasty.", "content": "Augmentation of the depressed nasal dorsum may be accomplished in a variety of ways by utilizing a number of different implant materials. Several techniques are discussed and illustrated. Small areas of depression may be corrected with viable or nonviable cartilage or synthetic implants through an intercartilaginous incision. More extensive saddling is best augmented using either iliac bone placed subperiosteally or irradiated cartilage. When the implant is sizable or a two piece \"L shaped\" strut is required, placement is done through a gingivolabial sulcus incision.", "contents": "Augmentation rhinoplasty. Augmentation of the depressed nasal dorsum may be accomplished in a variety of ways by utilizing a number of different implant materials. Several techniques are discussed and illustrated. Small areas of depression may be corrected with viable or nonviable cartilage or synthetic implants through an intercartilaginous incision. More extensive saddling is best augmented using either iliac bone placed subperiosteally or irradiated cartilage. When the implant is sizable or a two piece \"L shaped\" strut is required, placement is done through a gingivolabial sulcus incision."}
{"id": "PMID:1101151", "title": "Surgical stabilisation of the spine.", "content": "It is important to assess spinal injuries, both in terms of cord damage and column damage, shortly after the trauma has occurred. A decision should then be made concerning the eventual stability of the bony vertebral column. If a lesion is unlikely to heal soundly by bone or if one is unable to obtain a reduction by conservative measures, one should consider surgical stabilisation of the damaged spine in the subacute phase. At the same time as one stabilISES THe spine with metallic fixation, bone grafts should be added for ultimate stability. If an unstable spine is encountered in the later stages of treatment, surgical stabilisation is again indicated. Metallic fixation can greatly aid in stabilising the spine until bone grafts can mature. Osteotomies of the spine through a combined transthoracic and posterior approach can be extremely valuable in correcting deformity. While there is no role for decompressive surgery after spinal cord injury, there is still a definite role for surgery in balancing and stabilising the vertebral column.", "contents": "Surgical stabilisation of the spine. It is important to assess spinal injuries, both in terms of cord damage and column damage, shortly after the trauma has occurred. A decision should then be made concerning the eventual stability of the bony vertebral column. If a lesion is unlikely to heal soundly by bone or if one is unable to obtain a reduction by conservative measures, one should consider surgical stabilisation of the damaged spine in the subacute phase. At the same time as one stabilISES THe spine with metallic fixation, bone grafts should be added for ultimate stability. If an unstable spine is encountered in the later stages of treatment, surgical stabilisation is again indicated. Metallic fixation can greatly aid in stabilising the spine until bone grafts can mature. Osteotomies of the spine through a combined transthoracic and posterior approach can be extremely valuable in correcting deformity. While there is no role for decompressive surgery after spinal cord injury, there is still a definite role for surgery in balancing and stabilising the vertebral column."}
{"id": "PMID:1101152", "title": "Effect of cycloheximide on pregnenolone-16alpha-carbonitrile (PCN)-induced ultrastructural changes in the rat liver.", "content": "Cycloheximide (10 mg/100 g body weight i.v. twice), an inhibitor of protein synthesis, caused smooth endoplasmic reticulum (SER) accumulation in the hepatocytes of female rats. Pregnenolone-16alpha-carbonitrile (PCN), given p.o., had a similar effect. When cycloheximide was administered to PCN-treated rats, SER proliferation as well as myelin figure formation were noted.", "contents": "Effect of cycloheximide on pregnenolone-16alpha-carbonitrile (PCN)-induced ultrastructural changes in the rat liver. Cycloheximide (10 mg/100 g body weight i.v. twice), an inhibitor of protein synthesis, caused smooth endoplasmic reticulum (SER) accumulation in the hepatocytes of female rats. Pregnenolone-16alpha-carbonitrile (PCN), given p.o., had a similar effect. When cycloheximide was administered to PCN-treated rats, SER proliferation as well as myelin figure formation were noted."}
{"id": "PMID:1101153", "title": "The multiple causes of alpha1-antitrypsin deficiency.", "content": "Alpha1-antitrypsin deficiency is a genetic model that predisposes to pulmonary emphysema or a form of hepatic cirrhosis in man. The antitrypsin protein protects the tissues from proteolytic digestion by lysosomal proteases from inflammatory cells. The three causes of antitrypsin deficiency demonstrated to date are: 1) A defect in synthesis and release of the Z variant from the liver. 2) Presence of an inactive (null) gene for antitrypsin production, and 3) Increased lability of certain variants associated with the in vivo degradation of antitrypsin.", "contents": "The multiple causes of alpha1-antitrypsin deficiency. Alpha1-antitrypsin deficiency is a genetic model that predisposes to pulmonary emphysema or a form of hepatic cirrhosis in man. The antitrypsin protein protects the tissues from proteolytic digestion by lysosomal proteases from inflammatory cells. The three causes of antitrypsin deficiency demonstrated to date are: 1) A defect in synthesis and release of the Z variant from the liver. 2) Presence of an inactive (null) gene for antitrypsin production, and 3) Increased lability of certain variants associated with the in vivo degradation of antitrypsin."}
{"id": "PMID:1101154", "title": "Further examples confirming the existence of Pi null (Pi-).", "content": "The existence of the Pi- allele (Pi null) was envisaged by Talamo when he discovered a subject whose serum contained no alpha-1-antitrypsin. We were able to demonstrate that a single dose of this allele exists in three families which we studied. In a fourth family, the propositus carried this allele in duplicate (phenotype Pi- -), which agrees with the results of the quantitative analyses carried out on the parents. The importance of the Pi- allele is envisaged and the necessity of the genetic survey is generally essential to demonstrate its existence.", "contents": "Further examples confirming the existence of Pi null (Pi-). The existence of the Pi- allele (Pi null) was envisaged by Talamo when he discovered a subject whose serum contained no alpha-1-antitrypsin. We were able to demonstrate that a single dose of this allele exists in three families which we studied. In a fourth family, the propositus carried this allele in duplicate (phenotype Pi- -), which agrees with the results of the quantitative analyses carried out on the parents. The importance of the Pi- allele is envisaged and the necessity of the genetic survey is generally essential to demonstrate its existence."}
{"id": "PMID:1101155", "title": "[Phenotype Pi and alpha 1 antitrypsin deficiency in types A and B chronic obstructive bronchopneumopathies].", "content": "The authors studied 79 patients with chronic obstructive lung disease. (COLD) (FEV1/VC less than 70%). Patients with chronic asthma were excluded from the series. These patients with COLD were divided up into 2 groups, depending on radiological and clinical criteria: (1) Type A, primary emphysema, 24 patients; (2) Type B, chronic bronchitis, 49 patients. 6 patients of type X (intermediate type) were excluded. Their respiratory function tests were studied together with clinical and radiological findings and blood gases. Furthermore, were studied in each patient, the antitrypsic activity and the serum concentrations of alpha-1-antitrypsin and phenotype Pi. Group A were, on average, more obstructive than group B. The ratio FEV1/VC was 31% +/- 7% in group A, against 46% +/- 13% in group B. The serum antitrypsin activity and the serum concentration in alpha-1-AT were on average close to normal in both groups. However, group A was divided up into two populations: a small population with a low concentration of alpha-1-AT, and a large population with normal alpha-1-AT. A study of the incidence of various phenotypes in groups A and B showed a significantly higher frequency of phenotype ZZ in group A (12.5%) than in group B (10%). All ZZ subjects were of clinical type A. There was no significant difference in the incidence of other phenotypes encountered. Serum alpha-1-antitrypsin concentration was very low in 3 ZZ patients. One case with phenotype SZ and one MM showed intermediate levels. The authors emphasize the low percentage of cases of pulmonary emphysema which may be explained by an alpha-1-antitrypsin deficiency. Nevertheless, this preliminary report should be completed by a study of a larger sample of patients with COLD with control of the phenotype.", "contents": "[Phenotype Pi and alpha 1 antitrypsin deficiency in types A and B chronic obstructive bronchopneumopathies]. The authors studied 79 patients with chronic obstructive lung disease. (COLD) (FEV1/VC less than 70%). Patients with chronic asthma were excluded from the series. These patients with COLD were divided up into 2 groups, depending on radiological and clinical criteria: (1) Type A, primary emphysema, 24 patients; (2) Type B, chronic bronchitis, 49 patients. 6 patients of type X (intermediate type) were excluded. Their respiratory function tests were studied together with clinical and radiological findings and blood gases. Furthermore, were studied in each patient, the antitrypsic activity and the serum concentrations of alpha-1-antitrypsin and phenotype Pi. Group A were, on average, more obstructive than group B. The ratio FEV1/VC was 31% +/- 7% in group A, against 46% +/- 13% in group B. The serum antitrypsin activity and the serum concentration in alpha-1-AT were on average close to normal in both groups. However, group A was divided up into two populations: a small population with a low concentration of alpha-1-AT, and a large population with normal alpha-1-AT. A study of the incidence of various phenotypes in groups A and B showed a significantly higher frequency of phenotype ZZ in group A (12.5%) than in group B (10%). All ZZ subjects were of clinical type A. There was no significant difference in the incidence of other phenotypes encountered. Serum alpha-1-antitrypsin concentration was very low in 3 ZZ patients. One case with phenotype SZ and one MM showed intermediate levels. The authors emphasize the low percentage of cases of pulmonary emphysema which may be explained by an alpha-1-antitrypsin deficiency. Nevertheless, this preliminary report should be completed by a study of a larger sample of patients with COLD with control of the phenotype."}
{"id": "PMID:1101156", "title": "[Study on the relationship between serum alpha 1 antitrypsin level, the phenotype Pi and various bronchopulmonary diseases].", "content": "The authors determined in 418 adults, males and females, admitted to hospital on the Respiratory Unit of the Montpellier University Hospital, the serum levels of alpha-1-antitrypsin, phenotype Pi and serum immunoglobulin levels. They compare these data together and with the diagnosis using a mathematical method known as factorial correspondence analysis.", "contents": "[Study on the relationship between serum alpha 1 antitrypsin level, the phenotype Pi and various bronchopulmonary diseases]. The authors determined in 418 adults, males and females, admitted to hospital on the Respiratory Unit of the Montpellier University Hospital, the serum levels of alpha-1-antitrypsin, phenotype Pi and serum immunoglobulin levels. They compare these data together and with the diagnosis using a mathematical method known as factorial correspondence analysis."}
{"id": "PMID:1101157", "title": "Human blood platelet elastase and proelastase.", "content": "Platelet elastase has been differenciated from various protein fractions into a trypsin dependent form and a trypsin independent form. Trypsin independent elastase has been purified by affinity chromatography on cellulose elastin column as a pure protein raction of molecular weight: 26,000 ou SDS acrylamide gels. Trypsin dependent elastase has been purified by preparative acrylamide disc gel electrophoresis. This fraction, proteolysed (limited proteolysis) and activated by trypsin into active elastase, has been identified as the precursor (platelet proelastase) of platelet elastase. Its molecular weight is 28,000 before trypsin and 26,000 after trypsin.", "contents": "Human blood platelet elastase and proelastase. Platelet elastase has been differenciated from various protein fractions into a trypsin dependent form and a trypsin independent form. Trypsin independent elastase has been purified by affinity chromatography on cellulose elastin column as a pure protein raction of molecular weight: 26,000 ou SDS acrylamide gels. Trypsin dependent elastase has been purified by preparative acrylamide disc gel electrophoresis. This fraction, proteolysed (limited proteolysis) and activated by trypsin into active elastase, has been identified as the precursor (platelet proelastase) of platelet elastase. Its molecular weight is 28,000 before trypsin and 26,000 after trypsin."}
{"id": "PMID:1101158", "title": "[Coupled use of chromatography and acrylamide-agarose gel electrophoresis in the study of polymeric forms of paraproteins].", "content": "A study of the physical properties of paraprotein required until now, the use of fairly complicated techniques, such as analytical ultracentrifugation. The use of chromatography on acrylamide agar pearls and electrophoresis on indubiose plates, coupled with the classical techniques of electrophoresis and immunoelectrophoresis, permitted a more simple approach to these problems. The study of IgA paraproteins in several polymerised forms, is an example of the possibilities of this new method of investigation.", "contents": "[Coupled use of chromatography and acrylamide-agarose gel electrophoresis in the study of polymeric forms of paraproteins]. A study of the physical properties of paraprotein required until now, the use of fairly complicated techniques, such as analytical ultracentrifugation. The use of chromatography on acrylamide agar pearls and electrophoresis on indubiose plates, coupled with the classical techniques of electrophoresis and immunoelectrophoresis, permitted a more simple approach to these problems. The study of IgA paraproteins in several polymerised forms, is an example of the possibilities of this new method of investigation."}
{"id": "PMID:1101159", "title": "[Blood stock management by computers in a transfusion center].", "content": "In large blood Transfusion Centers, blood stock management is quite difficult because of the numerous daily movements, the short storage time of blood products, the multiplicity of sample types, and, indeed, of blood antigens, etc. A data processing system has been established in the Blood Transfusion Center ofLille (170 000 bottles a year). From information codified in collection and in distribution departments, the computer is able to give to the authorities essential daily decision elements such as the knowledge of :--the exact profile of each collection (1 119 in 1974),--statistics of blood distributions,--available stock, classified by storage time, volume, blood group, etc. The computer contribution from the security point of view, as well as its utilization in phenotyped blood research is described here.", "contents": "[Blood stock management by computers in a transfusion center]. In large blood Transfusion Centers, blood stock management is quite difficult because of the numerous daily movements, the short storage time of blood products, the multiplicity of sample types, and, indeed, of blood antigens, etc. A data processing system has been established in the Blood Transfusion Center ofLille (170 000 bottles a year). From information codified in collection and in distribution departments, the computer is able to give to the authorities essential daily decision elements such as the knowledge of :--the exact profile of each collection (1 119 in 1974),--statistics of blood distributions,--available stock, classified by storage time, volume, blood group, etc. The computer contribution from the security point of view, as well as its utilization in phenotyped blood research is described here."}
{"id": "PMID:1101160", "title": "[Value and duration of the bone localization of gallium in the mouse].", "content": "Gallium was administered subcutaneously to mice, and was found to be localized essentially in the skeleton. It was measured in the bones of animals given daily injections of gallium sulfate. There was little or no mortality. The amount of gallium found rose with the number of injections, and was greater when higher doses were injected. Elimination was very low after cessation of treatment. Results were used to study the action of gallium on cutaneous allografts in mice.", "contents": "[Value and duration of the bone localization of gallium in the mouse]. Gallium was administered subcutaneously to mice, and was found to be localized essentially in the skeleton. It was measured in the bones of animals given daily injections of gallium sulfate. There was little or no mortality. The amount of gallium found rose with the number of injections, and was greater when higher doses were injected. Elimination was very low after cessation of treatment. Results were used to study the action of gallium on cutaneous allografts in mice."}
{"id": "PMID:1101161", "title": "[Comparison of blood sugar measured by Hoffman's, glucose oxidase and dextrostix technics during the oral glucose tolerance test].", "content": "The venous blood sugar has been measured in 384 adult subjects, between 20 and 60 years if agem during two oral glocose tolerance tests, with three methods: Hoffman's method, Glucose-oxidase method, and assay with reflectance meter (DRM). A good correlation was found with all methods (erg = 0,6 at 60 mn after load); however, the results given by the DRM were lower. So, in spite of its practical and economical advantages, the DRM can neverbe used for the diagnosis of an anomaly in the OGTT results. This method is a good one in emergency situations and in mass screening for diabetes.", "contents": "[Comparison of blood sugar measured by Hoffman's, glucose oxidase and dextrostix technics during the oral glucose tolerance test]. The venous blood sugar has been measured in 384 adult subjects, between 20 and 60 years if agem during two oral glocose tolerance tests, with three methods: Hoffman's method, Glucose-oxidase method, and assay with reflectance meter (DRM). A good correlation was found with all methods (erg = 0,6 at 60 mn after load); however, the results given by the DRM were lower. So, in spite of its practical and economical advantages, the DRM can neverbe used for the diagnosis of an anomaly in the OGTT results. This method is a good one in emergency situations and in mass screening for diabetes."}
{"id": "PMID:1101162", "title": "[Normal ultrastructure of the small bronchi and bronchioli in man].", "content": "Ultrastructural topographic and morphological analysis of the small bronchi and bronchioli was carried out on selected biopsy specimens obtained from 6 operative specimens. The small bronchi had a ciliated cylindrical epithelium identical to that in the large bronchi, apart from a smaller number of goblet cells. Their reticulin layer was thin (1 to 3 microns). The glands in the submucosa were rare whereas there was a rich submucosal vascular network. The lobular bronchioli had a ciliated cylindrical epithelium without goblet cells but possessing rare Clara cells. The epithelium of the terminal bromchioli was characterized by: 1 - The presence of numerous Clara cells, generally grouped in groups of 2 or 3. 2 - The appearance on the opposite side of a satellite artery (this side shows alveoli first) of cells which we interpreted as pre-ciliated cells. These cells possess a range of basal corpuscles under the luminal membrane but do not have cilia. 3 - The appearance of small membranous pneumocytes in islets. The epithelium covering the smooth wall of the respiratory bronchioles still have a gutter of ciliated cells and Clara cells which become thinner towards the periphery. After the secondary respiratory bronchioli, there appears in this gutter a new cell type, the cubical cells. These cells which have poorly differentiated cytoplasmic characteristics, seem to us to be immature bronchial cells, precursors of the Clara cells rather than of the ciliated cells. The remainder of the smooth wall of the respiratory bronchioli is covered with membranous pneumocytes and, in the case of the 3rd order of bronchioli, also by granular pneumocytes. The sub-basal reticulin layer is lacking in the terminal and respiratory bronchioli. The total thickness of the wall becomes considerably thinner, the muscular layer becoming proportionally very thick.", "contents": "[Normal ultrastructure of the small bronchi and bronchioli in man]. Ultrastructural topographic and morphological analysis of the small bronchi and bronchioli was carried out on selected biopsy specimens obtained from 6 operative specimens. The small bronchi had a ciliated cylindrical epithelium identical to that in the large bronchi, apart from a smaller number of goblet cells. Their reticulin layer was thin (1 to 3 microns). The glands in the submucosa were rare whereas there was a rich submucosal vascular network. The lobular bronchioli had a ciliated cylindrical epithelium without goblet cells but possessing rare Clara cells. The epithelium of the terminal bromchioli was characterized by: 1 - The presence of numerous Clara cells, generally grouped in groups of 2 or 3. 2 - The appearance on the opposite side of a satellite artery (this side shows alveoli first) of cells which we interpreted as pre-ciliated cells. These cells possess a range of basal corpuscles under the luminal membrane but do not have cilia. 3 - The appearance of small membranous pneumocytes in islets. The epithelium covering the smooth wall of the respiratory bronchioles still have a gutter of ciliated cells and Clara cells which become thinner towards the periphery. After the secondary respiratory bronchioli, there appears in this gutter a new cell type, the cubical cells. These cells which have poorly differentiated cytoplasmic characteristics, seem to us to be immature bronchial cells, precursors of the Clara cells rather than of the ciliated cells. The remainder of the smooth wall of the respiratory bronchioli is covered with membranous pneumocytes and, in the case of the 3rd order of bronchioli, also by granular pneumocytes. The sub-basal reticulin layer is lacking in the terminal and respiratory bronchioli. The total thickness of the wall becomes considerably thinner, the muscular layer becoming proportionally very thick."}
{"id": "PMID:1101163", "title": "[Histiocytosis X. Histochemical study of intracytoplasmic lipids. Apropos of 3 cases].", "content": "Histochemical study combined with differential extractions of lipids from biopsy fragments in three cases of eosinophil granuloma reveals a great amount and variety of lipidic categories existing in histiocytosis X cells : sterids, fatty-acids, triacylglycerols, phosphoglycerids, cholino-phospholipids, sphingomyelins, glycolipids. Comparison of the lipidic content of histiocytosis X cells and that of normal histiocytes, showed a preponderance of cholesterol esters in histiocytosis X contrary to the normal macrophages and alteration in phospholipidic control of cholesterol esterification. This study confirms that the histiocytosis X cells have an active and probably preferential lipid metabolism, explaining the frequent xanthomatous changes of the lesions and the intense production of various cytoplasmic membranous structures.", "contents": "[Histiocytosis X. Histochemical study of intracytoplasmic lipids. Apropos of 3 cases]. Histochemical study combined with differential extractions of lipids from biopsy fragments in three cases of eosinophil granuloma reveals a great amount and variety of lipidic categories existing in histiocytosis X cells : sterids, fatty-acids, triacylglycerols, phosphoglycerids, cholino-phospholipids, sphingomyelins, glycolipids. Comparison of the lipidic content of histiocytosis X cells and that of normal histiocytes, showed a preponderance of cholesterol esters in histiocytosis X contrary to the normal macrophages and alteration in phospholipidic control of cholesterol esterification. This study confirms that the histiocytosis X cells have an active and probably preferential lipid metabolism, explaining the frequent xanthomatous changes of the lesions and the intense production of various cytoplasmic membranous structures."}
{"id": "PMID:1101165", "title": "[Automation of extraction in the determination of blood triglyceride].", "content": "A complete automation of the determination of triglycerides is described which replaces the manual extraction and the final determination with an autoanalyzer chain, based on the method of Royer et al. For the automation of triglyceride extraction, we have modified the sampler, by incorporating a magnetic stirrer under the sample table. The sample table has two rows of holes. The sera, which are taken from the inner row and diluted with sulphuric acid, are reacted quantitatively with the extraction reagent in the beakers of the outer row, which contain a magnetic follower. After stirring, the upper phase is quantitatively removed and the determination is started. The problem of phasing the individual steps of the analysis is thus solved.", "contents": "[Automation of extraction in the determination of blood triglyceride]. A complete automation of the determination of triglycerides is described which replaces the manual extraction and the final determination with an autoanalyzer chain, based on the method of Royer et al. For the automation of triglyceride extraction, we have modified the sampler, by incorporating a magnetic stirrer under the sample table. The sample table has two rows of holes. The sera, which are taken from the inner row and diluted with sulphuric acid, are reacted quantitatively with the extraction reagent in the beakers of the outer row, which contain a magnetic follower. After stirring, the upper phase is quantitatively removed and the determination is started. The problem of phasing the individual steps of the analysis is thus solved."}
{"id": "PMID:1101166", "title": "Peripheral nervous system involvement in experimental tuberculosis.", "content": "Following intravenous injection of mycobacteria in the mouse the bacilli are regularly found in the peripheral nervous system after the 5th day. The peripheral nerves and autonomic ganglia are involved at the level of the perineural cells and the sheath of Schwann. When the organisms are of low virulence (BCG, Mycobacterium intracellulare) the development of the nodules is limited. In the case of a virulent strain (Ravenel) extensive granulomas are found but epitheloid cells, always found in other organs, are constantly absent. In animals previously immunized, the bacilli seem to implant themselves as in the controls, but multiply more slowly; the nodules are more infrequent and the lymphoid infiltration appears earlier with the multiplication of interstitial cells.", "contents": "Peripheral nervous system involvement in experimental tuberculosis. Following intravenous injection of mycobacteria in the mouse the bacilli are regularly found in the peripheral nervous system after the 5th day. The peripheral nerves and autonomic ganglia are involved at the level of the perineural cells and the sheath of Schwann. When the organisms are of low virulence (BCG, Mycobacterium intracellulare) the development of the nodules is limited. In the case of a virulent strain (Ravenel) extensive granulomas are found but epitheloid cells, always found in other organs, are constantly absent. In animals previously immunized, the bacilli seem to implant themselves as in the controls, but multiply more slowly; the nodules are more infrequent and the lymphoid infiltration appears earlier with the multiplication of interstitial cells."}
{"id": "PMID:1101167", "title": "Inhibition of HBsAg immunologic reactivity and intestinal aerobic flora.", "content": "Cultures of human intestinal aerobic flora did not have any inhibiting action on the immunologic reactivity of hepatitis B antigen even after disruption of bacterial cells by freezing and thawing. This suggests that the inhibitor is elaborated by the human intestinal mucosa.", "contents": "Inhibition of HBsAg immunologic reactivity and intestinal aerobic flora. Cultures of human intestinal aerobic flora did not have any inhibiting action on the immunologic reactivity of hepatitis B antigen even after disruption of bacterial cells by freezing and thawing. This suggests that the inhibitor is elaborated by the human intestinal mucosa."}
{"id": "PMID:1101168", "title": "Cells involved in the immune response. XXIX Establishment of optimal conditions for the primary and secondary immune responses by rabbit lymphoid cells in vitro.", "content": "Attempts were made to initiate the primary and secondary humoral immune responses to sheep red blood cells (SRBC) in vitro as determined by the hemolytic plaque-forming cell (PFC) response, with cell suspensions prepared from a variety of lymphoid organs of the rabbit- thymus, bone marrow, spleen, appendix, sacculus rotundus, Peyer's patches, popliteal lymph node and circulating leukocytes. A number of different media and gaseous phases were utilized in order to establish the optimal conditions for the immune response in vitro. The induction of a secondary PFC response was consistently obtained with 'memory' spleen cells obtained from rabbits 3-6 months following intravenous immunization with SRBC but not with cells of any of the other lymphoid organs, and this response probably represents the activity of memory cells which reside in the rabbit spleen. A primary response was observed only with 'normal' spleen cells, and the medium which faciliated the response was different from that which facilitated the induction of the secondary response in vitro. It was also observed, using a medium in which normal spleen cells were incapable of generating PFC', that mixed cultures of normal spleen and normal appendix or bone marrow cells could give a marked PFC reponse in vitro. Whether the PFC response to SRBCs obtained with the lymphoid cells of normal, unimmunized rabbits represent a true primary response, a  secondary response, or a response of a different nature as a consequence of continuous subthreshold immunization of the rabbit with enteric microorganisms which cross-react with the antigen, remains to be determined. However, out initial successes with cultures consisting of cells of at least two distinct lymphoid organs in cases where the cells of any one of these organs could not respond, suggest that interaction of at least two functionally distinct cells is required and that the repsonse observed in vitro is probably a primary immune response.", "contents": "Cells involved in the immune response. XXIX Establishment of optimal conditions for the primary and secondary immune responses by rabbit lymphoid cells in vitro. Attempts were made to initiate the primary and secondary humoral immune responses to sheep red blood cells (SRBC) in vitro as determined by the hemolytic plaque-forming cell (PFC) response, with cell suspensions prepared from a variety of lymphoid organs of the rabbit- thymus, bone marrow, spleen, appendix, sacculus rotundus, Peyer's patches, popliteal lymph node and circulating leukocytes. A number of different media and gaseous phases were utilized in order to establish the optimal conditions for the immune response in vitro. The induction of a secondary PFC response was consistently obtained with 'memory' spleen cells obtained from rabbits 3-6 months following intravenous immunization with SRBC but not with cells of any of the other lymphoid organs, and this response probably represents the activity of memory cells which reside in the rabbit spleen. A primary response was observed only with 'normal' spleen cells, and the medium which faciliated the response was different from that which facilitated the induction of the secondary response in vitro. It was also observed, using a medium in which normal spleen cells were incapable of generating PFC', that mixed cultures of normal spleen and normal appendix or bone marrow cells could give a marked PFC reponse in vitro. Whether the PFC response to SRBCs obtained with the lymphoid cells of normal, unimmunized rabbits represent a true primary response, a  secondary response, or a response of a different nature as a consequence of continuous subthreshold immunization of the rabbit with enteric microorganisms which cross-react with the antigen, remains to be determined. However, out initial successes with cultures consisting of cells of at least two distinct lymphoid organs in cases where the cells of any one of these organs could not respond, suggest that interaction of at least two functionally distinct cells is required and that the repsonse observed in vitro is probably a primary immune response."}
{"id": "PMID:1101170", "title": "Pathologist and histotechnologist: a marriage in need of counseling.", "content": "The general level of histotechnology in this country is alarmingly low. Surgical pathology, a difficult field, is made doubly so by poorly prepared sections. The blame for this must rest in part on the pathologist because of his ignorance of histologic procedures, his acceptance of shoddy work, and his lack of interest in seeing that bright young people are enticed into the field and that adequate training facilities and career opportunities are available to them. Today we are asked to perform increasingly complex procedures, and if we are to meet this challenge, as well as perform our duties in pathologic anatomy to the best of our ability, it is essential that we rapidly change our attitude.", "contents": "Pathologist and histotechnologist: a marriage in need of counseling. The general level of histotechnology in this country is alarmingly low. Surgical pathology, a difficult field, is made doubly so by poorly prepared sections. The blame for this must rest in part on the pathologist because of his ignorance of histologic procedures, his acceptance of shoddy work, and his lack of interest in seeing that bright young people are enticed into the field and that adequate training facilities and career opportunities are available to them. Today we are asked to perform increasingly complex procedures, and if we are to meet this challenge, as well as perform our duties in pathologic anatomy to the best of our ability, it is essential that we rapidly change our attitude."}
{"id": "PMID:1101172", "title": "Pathogenesis of teratoid tumors of the ovary and testis.", "content": "Based upon a representative sample of testicular tumors studied at the Armed Forces Institute of Pathology, several testicular and ovarian tumors observed in Denver, pertinent papers in the literature, and the singular thesis of Chevassu on tumors of the testis, the pathogenesis of such neoplasms is elaborated. The findings are philosophical, speculative, and established. Man is a multicellular individual to be regarded as a vehicle for the transmission of unicellular organisms or germ cells from one generation to the next. These cells remain distinct from somatic and trophoblastic cells. The mature human female not only tolerates the normal expression of the fertilized ovum during pregnancy (sex cells, blastoderm, and trophoblast) but also seems capable of greater differentiation of immature somatic cells resulting from parthenogenesis of one or more ova into cells of the three germ layers, as well as the suppression of the growth of neoplastic sex cells and trophoblast cells, with benign cystic teratoma as the most common culmination. The preponderance of malignant teratoid tumors before sexual maturity is a corollary. In contrast, the human male is not equipped with organizers postulated for the human female and thus is unable to differentiate malignant immature somatic cells, the most common cancerous element in testicular tumors. The explanation for such neoplasms must be on the basis of segregation of such cells and abnormal spermatogonia or less often trophoblastic cells in the embryo, with later expression as neoplastic cells, since spermatogonia and progeny are unable to form a new individual. To paraphrase Wilms, the statement may be made that malignant testicular and ovarian tumors of teratoid type are related, despite their different microscopic appearance, to a common form. They differ only in the quality, not in the quantity, of the different tissues comprising them. These tumors contain neoplastic blastodermic cells and differentiated cells of the three germ layers, neoplastic sex cells, and neoplastic trophoblastic cells. The cells of these tumors and the tissues they form resemble very nearly the tissues of the human embryo with nonaxial formation of alimentary and respiratory structures in many instances. The notable frequency of variably differentiated neural elements in the teratoids tumors of the ovary is in sharp contrast to their uncommon occurrence in like tumors of the testis. Dysgenesis of the ovaries and the testes of testicular feminization syndrome should be regarded as likely soil for the development of teratoid tumors.", "contents": "Pathogenesis of teratoid tumors of the ovary and testis. Based upon a representative sample of testicular tumors studied at the Armed Forces Institute of Pathology, several testicular and ovarian tumors observed in Denver, pertinent papers in the literature, and the singular thesis of Chevassu on tumors of the testis, the pathogenesis of such neoplasms is elaborated. The findings are philosophical, speculative, and established. Man is a multicellular individual to be regarded as a vehicle for the transmission of unicellular organisms or germ cells from one generation to the next. These cells remain distinct from somatic and trophoblastic cells. The mature human female not only tolerates the normal expression of the fertilized ovum during pregnancy (sex cells, blastoderm, and trophoblast) but also seems capable of greater differentiation of immature somatic cells resulting from parthenogenesis of one or more ova into cells of the three germ layers, as well as the suppression of the growth of neoplastic sex cells and trophoblast cells, with benign cystic teratoma as the most common culmination. The preponderance of malignant teratoid tumors before sexual maturity is a corollary. In contrast, the human male is not equipped with organizers postulated for the human female and thus is unable to differentiate malignant immature somatic cells, the most common cancerous element in testicular tumors. The explanation for such neoplasms must be on the basis of segregation of such cells and abnormal spermatogonia or less often trophoblastic cells in the embryo, with later expression as neoplastic cells, since spermatogonia and progeny are unable to form a new individual. To paraphrase Wilms, the statement may be made that malignant testicular and ovarian tumors of teratoid type are related, despite their different microscopic appearance, to a common form. They differ only in the quality, not in the quantity, of the different tissues comprising them. These tumors contain neoplastic blastodermic cells and differentiated cells of the three germ layers, neoplastic sex cells, and neoplastic trophoblastic cells. The cells of these tumors and the tissues they form resemble very nearly the tissues of the human embryo with nonaxial formation of alimentary and respiratory structures in many instances. The notable frequency of variably differentiated neural elements in the teratoids tumors of the ovary is in sharp contrast to their uncommon occurrence in like tumors of the testis. Dysgenesis of the ovaries and the testes of testicular feminization syndrome should be regarded as likely soil for the development of teratoid tumors."}
{"id": "PMID:1101174", "title": "Idiopathic arterial calcification of infancy: a clinicopathologic study.", "content": "The clinical and pathologic features of a group of 62 infants dying of idiopathic calcific arterial disease were reviewed. The disease most commonly occurs in infants less than 6 months of age. Pathologically, it is characterized by calcific deposits along the internal elastic membrane of arteries accompanied by fibrous thickening of the intima which causes luminal narrowing. The arterial lesions are widespread but the resultant luminal narrowing invariably promotes myocardial ischemia, causing the infants' deaths. A definite tendency of the disease to occur in siblings has been noted, but additional patterns of inheritance are not yet apparent. Clinical diagnosis is feasible with radiologic study of arteries of the head, neck, and extremities. There is a similarity of idiopathic calcific arterial disease of infancy to the arterial lesions of metastatic calcification in severe renal disease, calcific arterial lesions noted in conjunction with certain cardiovascular anomalies, and hypervitaminosis D. Certain experimental situations and toxic states can also produce calcific and proliferative vascular lesions.", "contents": "Idiopathic arterial calcification of infancy: a clinicopathologic study. The clinical and pathologic features of a group of 62 infants dying of idiopathic calcific arterial disease were reviewed. The disease most commonly occurs in infants less than 6 months of age. Pathologically, it is characterized by calcific deposits along the internal elastic membrane of arteries accompanied by fibrous thickening of the intima which causes luminal narrowing. The arterial lesions are widespread but the resultant luminal narrowing invariably promotes myocardial ischemia, causing the infants' deaths. A definite tendency of the disease to occur in siblings has been noted, but additional patterns of inheritance are not yet apparent. Clinical diagnosis is feasible with radiologic study of arteries of the head, neck, and extremities. There is a similarity of idiopathic calcific arterial disease of infancy to the arterial lesions of metastatic calcification in severe renal disease, calcific arterial lesions noted in conjunction with certain cardiovascular anomalies, and hypervitaminosis D. Certain experimental situations and toxic states can also produce calcific and proliferative vascular lesions."}
{"id": "PMID:1101176", "title": "Management problems of mentally retarded children and their families.", "content": "Pediatricians faced with the difficult task of providing management services to mentally retarded children and their families are confronted with a number of difficult problems. These range all the way from deciding how to inform parents that their child is retarded to dealing with grandparents who are a source of stress. Successful handling of these problems requires a recognition that management is central to the care of the mentally retarded child. The needs of mentally retarded children and their families will not be met by interacting with families only around issues of acute physical illness. Attention must be directed toward psychosocial as well as medical variables and the emphasis must be on the total family system.", "contents": "Management problems of mentally retarded children and their families. Pediatricians faced with the difficult task of providing management services to mentally retarded children and their families are confronted with a number of difficult problems. These range all the way from deciding how to inform parents that their child is retarded to dealing with grandparents who are a source of stress. Successful handling of these problems requires a recognition that management is central to the care of the mentally retarded child. The needs of mentally retarded children and their families will not be met by interacting with families only around issues of acute physical illness. Attention must be directed toward psychosocial as well as medical variables and the emphasis must be on the total family system."}
{"id": "PMID:1101177", "title": "Common sexual problems of children and adolescents.", "content": "The pediatrician is often expected by families to deal with sexual problems of children and adolescents. The physician should be able to identify problems and to guide parents in more meaningful communication and education of their children. Hopefully, he will be able to identify family conflicts and make the appropriate intervention or referral. He should be aware of his own sexual attitudes so as to avoid having his bias interfere with treatment. The childs behavior should not be isolated, but considered in the context of his family, his peers, and his own growth and development. Often, it is more useful to advise the family than to work with a young child. Adolescents present particular problems because of their conflicts over sexual identity, their reluctance to admit to problems, and frequently a mistrust of adults. They often feel a need for a trusting relationship with an adult, however, and are able to relate to a sensitive, non-judgmental professional.", "contents": "Common sexual problems of children and adolescents. The pediatrician is often expected by families to deal with sexual problems of children and adolescents. The physician should be able to identify problems and to guide parents in more meaningful communication and education of their children. Hopefully, he will be able to identify family conflicts and make the appropriate intervention or referral. He should be aware of his own sexual attitudes so as to avoid having his bias interfere with treatment. The childs behavior should not be isolated, but considered in the context of his family, his peers, and his own growth and development. Often, it is more useful to advise the family than to work with a young child. Adolescents present particular problems because of their conflicts over sexual identity, their reluctance to admit to problems, and frequently a mistrust of adults. They often feel a need for a trusting relationship with an adult, however, and are able to relate to a sensitive, non-judgmental professional."}
{"id": "PMID:1101179", "title": "Symposium on behavioral pediatrics. Juvenile delinquency.", "content": "The pediatrician's first step in responding to delinquent behavior is to make a differential diagnosis between sociological, characterological and neurotic delinquency. Sociological delinquency consists of subculturally sanctioned illegal activity by youngsters who are psychologically well-adjusted members of their peer group, and its treatment depends primarily on prevention through social action programs rather than on the intervention of the clinical practitioner. Characterological delinquency reflects a serious and long-standing personality disorder marked by aggressive and impulsive behavior and a lack of attachment to or consideration for others, and its treatment requires extensive psychotherapy, often in a residential setting. Neurotic delinquency involves uncharacteristic misbehavior of recent origin that represents an attempt to communicate unrecognized needs for attention or help, and it can often be treated effectively with short-term psychotherapy.", "contents": "Symposium on behavioral pediatrics. Juvenile delinquency. The pediatrician's first step in responding to delinquent behavior is to make a differential diagnosis between sociological, characterological and neurotic delinquency. Sociological delinquency consists of subculturally sanctioned illegal activity by youngsters who are psychologically well-adjusted members of their peer group, and its treatment depends primarily on prevention through social action programs rather than on the intervention of the clinical practitioner. Characterological delinquency reflects a serious and long-standing personality disorder marked by aggressive and impulsive behavior and a lack of attachment to or consideration for others, and its treatment requires extensive psychotherapy, often in a residential setting. Neurotic delinquency involves uncharacteristic misbehavior of recent origin that represents an attempt to communicate unrecognized needs for attention or help, and it can often be treated effectively with short-term psychotherapy."}
{"id": "PMID:1101182", "title": "Decreased bactericidal activity of leukocytes of stressed newborn infants.", "content": "Previous studies have established that leukocyte phagocytosis and intracellular killing are normal in term and low-birthweight newborns who are well. To determine the effect of stress or illness on newborn leukocyte function, the phagocytic and bactericidal activity of leukocytes from 40 sick newborns was compared with that of leukocytes from 12 newborns and 23 normal adults. To eliminate abnormal phagocytosis resulting from serum opsonic defects in newborn sera, pooled adult sera were used in all assays. Twenty-five of the 40 stressed newborns (63%) had decreased in vitro activity against either Staphylococcus aureus 502A or Escherichia coli, or both, compared with decreased activity in two of 12 well infants (17%) and in four of 23 adult controls (17%). The mean bactericidal activity (percentage of organisms killed after two hours) of leukocytes from stressed newborns against S. aureus (83% +/- 2 [SEM]) and E. coli. (87% +/- 4 [SEM]) was significantly less than in the combined well infant and adult control group (94% +/- 1 for S. aureus and 97% +/- .5 for E. coli). Although the more severely ill infants had an increased incidence of impaired antibacterial activity, the degree of impairment was not related to the severity of illness. No consistent relationship of decreased activity to birthweight, gestational age, age when studied, or specific diagnosis was seen. The leukocyte abnormality in stressed infants against S. aureus was principally a killing defect, while against E. coli both phagocytosis and killing were abnormal. This study indicates that a wide variety of neonatal disorders may affect one or more of the steps required for normal bacterial killing. The lability of leukocytic antibacterial function under stress is an additional mechanism for the newborn's increased susceptibility to infection.", "contents": "Decreased bactericidal activity of leukocytes of stressed newborn infants. Previous studies have established that leukocyte phagocytosis and intracellular killing are normal in term and low-birthweight newborns who are well. To determine the effect of stress or illness on newborn leukocyte function, the phagocytic and bactericidal activity of leukocytes from 40 sick newborns was compared with that of leukocytes from 12 newborns and 23 normal adults. To eliminate abnormal phagocytosis resulting from serum opsonic defects in newborn sera, pooled adult sera were used in all assays. Twenty-five of the 40 stressed newborns (63%) had decreased in vitro activity against either Staphylococcus aureus 502A or Escherichia coli, or both, compared with decreased activity in two of 12 well infants (17%) and in four of 23 adult controls (17%). The mean bactericidal activity (percentage of organisms killed after two hours) of leukocytes from stressed newborns against S. aureus (83% +/- 2 [SEM]) and E. coli. (87% +/- 4 [SEM]) was significantly less than in the combined well infant and adult control group (94% +/- 1 for S. aureus and 97% +/- .5 for E. coli). Although the more severely ill infants had an increased incidence of impaired antibacterial activity, the degree of impairment was not related to the severity of illness. No consistent relationship of decreased activity to birthweight, gestational age, age when studied, or specific diagnosis was seen. The leukocyte abnormality in stressed infants against S. aureus was principally a killing defect, while against E. coli both phagocytosis and killing were abnormal. This study indicates that a wide variety of neonatal disorders may affect one or more of the steps required for normal bacterial killing. The lability of leukocytic antibacterial function under stress is an additional mechanism for the newborn's increased susceptibility to infection."}
{"id": "PMID:1101186", "title": "Human figure drawing characteristics related to juvenile delinquents.", "content": "Human figure drawings were scored on seven characteristics popularly attributed to juvenile delinquents, i.e., head size, shading, and three indicators of emotional conflict, i.e., transparencies, omissions, and erasures. Ss were adolescents incarcerated at training schools. Delinquency was defined in terms of MMPI profiles associated with delinquency rather than by social-legal or medical-diagnostic classification. The results fail to support popular hypotheses concerning human figure drawings of delinquents. Delinquents drew significantly more transparencies; however, no other emotional indicator was significant. It was concluded that little support exists for hypotheses concerning human figure drawings of delinquents and drawings should be used with caution in the clinical assessment of a delinquent's personality.", "contents": "Human figure drawing characteristics related to juvenile delinquents. Human figure drawings were scored on seven characteristics popularly attributed to juvenile delinquents, i.e., head size, shading, and three indicators of emotional conflict, i.e., transparencies, omissions, and erasures. Ss were adolescents incarcerated at training schools. Delinquency was defined in terms of MMPI profiles associated with delinquency rather than by social-legal or medical-diagnostic classification. The results fail to support popular hypotheses concerning human figure drawings of delinquents. Delinquents drew significantly more transparencies; however, no other emotional indicator was significant. It was concluded that little support exists for hypotheses concerning human figure drawings of delinquents and drawings should be used with caution in the clinical assessment of a delinquent's personality."}
{"id": "PMID:1101188", "title": "Sleep during Transcendental Meditation.", "content": "Electroencephalograms (EEGs) and DC electrooculograms (EOGs) were recorded during Transcendental Meditation periods for 8 experienced Ss. The records, scored blind showed that all but 2 Ss spent considerable portions of their meditation periods in unambiguous physiological sleep.", "contents": "Sleep during Transcendental Meditation. Electroencephalograms (EEGs) and DC electrooculograms (EOGs) were recorded during Transcendental Meditation periods for 8 experienced Ss. The records, scored blind showed that all but 2 Ss spent considerable portions of their meditation periods in unambiguous physiological sleep."}
{"id": "PMID:1101191", "title": "A new method for toilet training developmentally disabled children.", "content": "20 profoundly retarded children (4 to 12 yr.) were trained, using a variety of relaxation and tension activities designed to help them differentiate and gain control of the toileting musculature. Operant techniques were used to reinforce appropriate urination. The post-training scores of the experimental and control groups differed significantly for both accidental and appropriate urination.", "contents": "A new method for toilet training developmentally disabled children. 20 profoundly retarded children (4 to 12 yr.) were trained, using a variety of relaxation and tension activities designed to help them differentiate and gain control of the toileting musculature. Operant techniques were used to reinforce appropriate urination. The post-training scores of the experimental and control groups differed significantly for both accidental and appropriate urination."}
{"id": "PMID:1101198", "title": "Prostaglandin induced parturition in swine with some aspects on prevention of the MMA (metritis, mastitis, agalactia) syndrome.", "content": "The effect of PGF2alpha to induce parturition in sows was studied in two herds. In one herd with a total number of 150 sows and with a good health status 12 sows were injected intramuscularly with 12.5 mg PGF2alpha during late pregnancy (110--114 days). During the same period 12 sows were treated intramuscularly with saline solution at corresponding gestation stage. The results are given in Tables I and II. The mean age calculated as number of litters was 2.6 in the experimental group and 4.3 in the control group. All sows treated with PGF2alpha farrowed within 30 hours after injection at an average time of 25.4 hours. The controls farrowed at an average 80.2 hours after injection. The mean farrowing time was 4.4 hours for the PG-treated sows while the corresponding figure for the controls was 6.6 hours. The litter size at birth, after 3 weeks and at weaning was somewhat higher in the experimental group. The same applies to the litter weight at the age of 3 weeks. The course of parturition was normal in all sows and the health condition during lactation was good. In another herd -- numbering 18 sows -- all sows which hade farrowed during the last 4 months before the present investigation, had developed the Mastitis-Metritis-Agalactia syndrome (MMA). The symptoms were a prolonged gestation length (116--117 days), fever, mastitis and agalactia. During the present study 3 sows were left untreated while 5 sows were given an intramuscular injection with 12.5 mg PGF2alpha during late pregnancy (112--114 days). Four of the five PG-treated sows farrowed within 32 hours, while the fifth sow farrowed 100 hours after injection. The sow not responding to the PG-treatment and the untreated sows all developed MMA while the four sows responding to treatment remained healthy. The gestation length in treated sows responding to treatment was 113--115 days. In the sows developing MMA the gestation length was 116--117 days. The potential effect for prevention of MMA of shortening the gestation length in this herd is discussed.", "contents": "Prostaglandin induced parturition in swine with some aspects on prevention of the MMA (metritis, mastitis, agalactia) syndrome. The effect of PGF2alpha to induce parturition in sows was studied in two herds. In one herd with a total number of 150 sows and with a good health status 12 sows were injected intramuscularly with 12.5 mg PGF2alpha during late pregnancy (110--114 days). During the same period 12 sows were treated intramuscularly with saline solution at corresponding gestation stage. The results are given in Tables I and II. The mean age calculated as number of litters was 2.6 in the experimental group and 4.3 in the control group. All sows treated with PGF2alpha farrowed within 30 hours after injection at an average time of 25.4 hours. The controls farrowed at an average 80.2 hours after injection. The mean farrowing time was 4.4 hours for the PG-treated sows while the corresponding figure for the controls was 6.6 hours. The litter size at birth, after 3 weeks and at weaning was somewhat higher in the experimental group. The same applies to the litter weight at the age of 3 weeks. The course of parturition was normal in all sows and the health condition during lactation was good. In another herd -- numbering 18 sows -- all sows which hade farrowed during the last 4 months before the present investigation, had developed the Mastitis-Metritis-Agalactia syndrome (MMA). The symptoms were a prolonged gestation length (116--117 days), fever, mastitis and agalactia. During the present study 3 sows were left untreated while 5 sows were given an intramuscular injection with 12.5 mg PGF2alpha during late pregnancy (112--114 days). Four of the five PG-treated sows farrowed within 32 hours, while the fifth sow farrowed 100 hours after injection. The sow not responding to the PG-treatment and the untreated sows all developed MMA while the four sows responding to treatment remained healthy. The gestation length in treated sows responding to treatment was 113--115 days. In the sows developing MMA the gestation length was 116--117 days. The potential effect for prevention of MMA of shortening the gestation length in this herd is discussed."}
{"id": "PMID:1101206", "title": "[Immunofluorescence study of bacteriuria. A new technic for the localization of urinary infection].", "content": "The detection by immunofluorescence of immunoglobulins fixed to the wall of urinary bacteria makes it possible to localise the site of the urinary infection. The presence of immunoglobulins was noted in 18 out of 19 patients with a high urinary tract infection involving the renal parenchyma. They were absent in 25 out of 28 subjects with a lower urinary tract infection. The three apparently discordant positive reaction involved 1 case of prostatitis and two cases in which the diagnosis of pyelonephritis was made later. In the case of renal involvement, these immunoglobulins, essentially IgG, are seen with greater frequency than humoral antibodies. Whilst the biological significance of these immunoglobulins remains uncertain, their existence probably being a reflection of parenchymatous inflammation, this nevertheless is a new and, apparently, reliable, method for the determination of the site of an infective process.", "contents": "[Immunofluorescence study of bacteriuria. A new technic for the localization of urinary infection]. The detection by immunofluorescence of immunoglobulins fixed to the wall of urinary bacteria makes it possible to localise the site of the urinary infection. The presence of immunoglobulins was noted in 18 out of 19 patients with a high urinary tract infection involving the renal parenchyma. They were absent in 25 out of 28 subjects with a lower urinary tract infection. The three apparently discordant positive reaction involved 1 case of prostatitis and two cases in which the diagnosis of pyelonephritis was made later. In the case of renal involvement, these immunoglobulins, essentially IgG, are seen with greater frequency than humoral antibodies. Whilst the biological significance of these immunoglobulins remains uncertain, their existence probably being a reflection of parenchymatous inflammation, this nevertheless is a new and, apparently, reliable, method for the determination of the site of an infective process."}
{"id": "PMID:1101207", "title": "[Surgical treatment of the non suppurated sacrococcygeal cyst. Technical improvements].", "content": "Some justified technical improvements have been made to the method of treatment for non-infected and sacro-coccygienally located \"pilo-nidal\". The absence of tension due to the use of a rotational autoplasty and the drying of the residual cavity by an efficient aspirative drainage are the means essentially employed.", "contents": "[Surgical treatment of the non suppurated sacrococcygeal cyst. Technical improvements]. Some justified technical improvements have been made to the method of treatment for non-infected and sacro-coccygienally located \"pilo-nidal\". The absence of tension due to the use of a rotational autoplasty and the drying of the residual cavity by an efficient aspirative drainage are the means essentially employed."}
{"id": "PMID:1101212", "title": "[Treatment of arteriovenous fistulas and of aneurysms using an inflatable and releasable balloon. Experimental principles. Application to man].", "content": "The authors have perfected a technique making possible the percutaneous introduction of a balloon filled with hydrosoluble iodine and releasable, either in an arteriovenous fistula or in an aneurysm. Experimental jugulo-carotid fistulae and experimental aneurysms in the dog were treated in this way. The technique has been used in two human patients - one with a traumatic caverno-carotid fistula and the other with a complicated vertebral fistula.", "contents": "[Treatment of arteriovenous fistulas and of aneurysms using an inflatable and releasable balloon. Experimental principles. Application to man]. The authors have perfected a technique making possible the percutaneous introduction of a balloon filled with hydrosoluble iodine and releasable, either in an arteriovenous fistula or in an aneurysm. Experimental jugulo-carotid fistulae and experimental aneurysms in the dog were treated in this way. The technique has been used in two human patients - one with a traumatic caverno-carotid fistula and the other with a complicated vertebral fistula."}
{"id": "PMID:1101220", "title": "[Current problems of data processing in clinical nuclear medicine. Symposium of Rhine-Westphalian Society for Nuclear Medicine in Bonn, Dezember 7, 1974].", "content": "Practical aspects of the use of computer systems in Nuclear Medicine were discussed in a symposium sponsored by the \"Rheinisch-Westf\u00e4lische Gesellschaft f\u00fcr Nuklearmedizin\" which was held at Bonn in December '74. In the opening session the president of the Society, Prof. Winkler (Bonn), gave a brief survey upon the development of data processing procedures for radionuclide diagnostics. He pointed out that at present computer application is not only of essential significance for camera scintigraphy but also for most of the other test methods as well as for the solution of organizing problems when running departments of Nuclear Medicine. For the performance of all these tasks and also for scientific purposes a special process control computer system has been developed in the Institute of Nuclear Medicine at the University of Bonn. A detailed description of this system was given by R. Knopp (Bonn). He emphasized in his paper that the rather bulky Bonn System, which consists of a dual computer (Siemens 305/306) and an extensive operating system, can not be regarded as a standard device for routine use in hospitals. However, on the basis of experiences which have been gathered on hand of the research system in Bonn efforts are in full activity for developing a compact Nuclear Medicine Computer system (CNMCS) suitable for the application within departments of any size. The CNMCS (which will be commercially available in the near future) is based on a 64 K, 16 bit central unit with a highly flexible operating system which permits a simultaneous multi user application of the computer. For CNMCS a comprehensive user software package will be at the clinician's disposal which can be easily handled.", "contents": "[Current problems of data processing in clinical nuclear medicine. Symposium of Rhine-Westphalian Society for Nuclear Medicine in Bonn, Dezember 7, 1974]. Practical aspects of the use of computer systems in Nuclear Medicine were discussed in a symposium sponsored by the \"Rheinisch-Westf\u00e4lische Gesellschaft f\u00fcr Nuklearmedizin\" which was held at Bonn in December '74. In the opening session the president of the Society, Prof. Winkler (Bonn), gave a brief survey upon the development of data processing procedures for radionuclide diagnostics. He pointed out that at present computer application is not only of essential significance for camera scintigraphy but also for most of the other test methods as well as for the solution of organizing problems when running departments of Nuclear Medicine. For the performance of all these tasks and also for scientific purposes a special process control computer system has been developed in the Institute of Nuclear Medicine at the University of Bonn. A detailed description of this system was given by R. Knopp (Bonn). He emphasized in his paper that the rather bulky Bonn System, which consists of a dual computer (Siemens 305/306) and an extensive operating system, can not be regarded as a standard device for routine use in hospitals. However, on the basis of experiences which have been gathered on hand of the research system in Bonn efforts are in full activity for developing a compact Nuclear Medicine Computer system (CNMCS) suitable for the application within departments of any size. The CNMCS (which will be commercially available in the near future) is based on a 64 K, 16 bit central unit with a highly flexible operating system which permits a simultaneous multi user application of the computer. For CNMCS a comprehensive user software package will be at the clinician's disposal which can be easily handled."}
{"id": "PMID:1101221", "title": "A chemical approach to studies of the three-dimensional structure of tRNA - alkylation with a reagent covalently bound to a \"peculiar site'.", "content": "Yeast valine tRNA1 was chemically modified with chlorambucil N-hydroxysuccinimide ester. tthe reagent was attached covalently to the valine residue of valyl-tRNA1Val under the conditions which prevented tRNA from alkylation. Chlorambucilyl-valyl-tRNA1Val thus obtained was separated from excess reagent and incubated in an aqueous solution at neutral pH in the presence of Mg++ions. Highly efficient intramolecular self-alkylation of chlorambucilyl-valyl-tRNA1Val took place. The chlorambucil residue bound covalently to the amino group of the valine residue of tRNA1Val alkylates the 5'-terminal phosphate group of the molecule, and its 3'-terminal sequence -A-C-C-A.", "contents": "A chemical approach to studies of the three-dimensional structure of tRNA - alkylation with a reagent covalently bound to a \"peculiar site'. Yeast valine tRNA1 was chemically modified with chlorambucil N-hydroxysuccinimide ester. tthe reagent was attached covalently to the valine residue of valyl-tRNA1Val under the conditions which prevented tRNA from alkylation. Chlorambucilyl-valyl-tRNA1Val thus obtained was separated from excess reagent and incubated in an aqueous solution at neutral pH in the presence of Mg++ions. Highly efficient intramolecular self-alkylation of chlorambucilyl-valyl-tRNA1Val took place. The chlorambucil residue bound covalently to the amino group of the valine residue of tRNA1Val alkylates the 5'-terminal phosphate group of the molecule, and its 3'-terminal sequence -A-C-C-A."}
{"id": "PMID:1101222", "title": "A model for chromatin structure.", "content": "A model for chromatin structure is presented. (a) Each of four histone species, H2A (IIbl or f2a2), H2B (IIb2 or f2b), H3 (III or f3) and H4 (IV or f2al) can form a parallel dimer. (b) These dimers can form two tetramers, (H2A)2(H2b)2 and (H3)2(H4)2. (C) These two tetramers bind a segment of DNA and condense it into a \"C\" segments. (d) The adjacent segments, termed extended or \"E\" segments, are bound by histone H1 (I or fl) for the major fraction of chromatin; the other \"E\" regions can be either bound by non-histone proteins or free of protein binding. (e) The binding of histones causes a structural distortion of the DNA which, depending upon the external conditions, may generate the formation of either an  open structure with a heterogeneous and non-uniform supercoil or a compact structure with a string of beads. The model is supported by experimental data on histone-histone interaction, histone-DNA interaction and histone subunit-DNA interaction.", "contents": "A model for chromatin structure. A model for chromatin structure is presented. (a) Each of four histone species, H2A (IIbl or f2a2), H2B (IIb2 or f2b), H3 (III or f3) and H4 (IV or f2al) can form a parallel dimer. (b) These dimers can form two tetramers, (H2A)2(H2b)2 and (H3)2(H4)2. (C) These two tetramers bind a segment of DNA and condense it into a \"C\" segments. (d) The adjacent segments, termed extended or \"E\" segments, are bound by histone H1 (I or fl) for the major fraction of chromatin; the other \"E\" regions can be either bound by non-histone proteins or free of protein binding. (e) The binding of histones causes a structural distortion of the DNA which, depending upon the external conditions, may generate the formation of either an  open structure with a heterogeneous and non-uniform supercoil or a compact structure with a string of beads. The model is supported by experimental data on histone-histone interaction, histone-DNA interaction and histone subunit-DNA interaction."}
{"id": "PMID:1101223", "title": "Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro.", "content": "(Deoxyribonucleic acid from Micrococcus luteus was methylated in vitro in the presence of S-adenosyl-(14C methyl)methionine with a DNA methyltransferase purified from extracts of te. coli infected with bacteriophage T2. The labelled DNA was degraded by enzymatic and specific chemical methods and the resulting short oligonucleotides were separated and characterized. tthe analytical data permit the conclusion that the tdna transmethylase reacts specifically with N-G-A-T-C-N sequences in which it converts adenine to a 6-methyl-aminopurine residue.", "contents": "Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro. (Deoxyribonucleic acid from Micrococcus luteus was methylated in vitro in the presence of S-adenosyl-(14C methyl)methionine with a DNA methyltransferase purified from extracts of te. coli infected with bacteriophage T2. The labelled DNA was degraded by enzymatic and specific chemical methods and the resulting short oligonucleotides were separated and characterized. tthe analytical data permit the conclusion that the tdna transmethylase reacts specifically with N-G-A-T-C-N sequences in which it converts adenine to a 6-methyl-aminopurine residue."}
{"id": "PMID:1101224", "title": "tRNA tertiary structure in solution as probed by the photochemically induced 8-13 cross-link.", "content": "The conformation of ten purified tRNAs from Escherichia Coli has been investigated by means of the photo-induced cross-linking of 4Srd8 and Cyd13, which is sensitive to the juxtaposition of the two bases. Three tRNAs photo-react abnormally slowly; tRNAPhe, tRNAMet/m a and tRNAVal/2; a comparison with normally reacting species suggests that base 47 (Urd or modified Urd) is involved in a tertiary interaction in Class I tRNAs with the triplet 8, 14, 28. The UGA suppressor tRNATrp photoreacts significantly slower than the wild type. Thus the single base change Gua 24 to Ade induces a conformational change that alters the rate constant for the cross-linking reaction.", "contents": "tRNA tertiary structure in solution as probed by the photochemically induced 8-13 cross-link. The conformation of ten purified tRNAs from Escherichia Coli has been investigated by means of the photo-induced cross-linking of 4Srd8 and Cyd13, which is sensitive to the juxtaposition of the two bases. Three tRNAs photo-react abnormally slowly; tRNAPhe, tRNAMet/m a and tRNAVal/2; a comparison with normally reacting species suggests that base 47 (Urd or modified Urd) is involved in a tertiary interaction in Class I tRNAs with the triplet 8, 14, 28. The UGA suppressor tRNATrp photoreacts significantly slower than the wild type. Thus the single base change Gua 24 to Ade induces a conformational change that alters the rate constant for the cross-linking reaction."}
{"id": "PMID:1101225", "title": "Utilization of an Escherichia coli mutant for carbon-13 enrichment of tRNA for NMR studies.", "content": "The enrichment of tRNA at specific sites with carbon-13 has been accomplished in vivo using a mutant of Escherichia coli. A relaxed strain of E. coli auxotrophic for methionine was grown in a specifically defined medium supplemented with either [14C] or [13C]-methyl labeled methionine. Cells were collected at the end of the log-phase of growth and tRNA was extracted. Analysis of the radioactivity of the [14C]-labeled tRNA established an incorporation ratio of three labeled carbons per tRNA molecule. Incorporation of the [14C]-label in vivo was confined to the methylation of nucleotides as determined by thin layer chromatography of nucleotides resulting from a ribonuclease digestion of [14C]-labeled tRNA. The carbon-13 NMR spectrum of [13C]-enriched tRNA indicated a similar degree of incorporation into the methylated nucleotides by the substantial enhancement of [13C]-methyl NMR signals only. Assignment of signals has been made for the methyl groups of ribothymidine and N7-methylguanosine in E. coli tRNA.", "contents": "Utilization of an Escherichia coli mutant for carbon-13 enrichment of tRNA for NMR studies. The enrichment of tRNA at specific sites with carbon-13 has been accomplished in vivo using a mutant of Escherichia coli. A relaxed strain of E. coli auxotrophic for methionine was grown in a specifically defined medium supplemented with either [14C] or [13C]-methyl labeled methionine. Cells were collected at the end of the log-phase of growth and tRNA was extracted. Analysis of the radioactivity of the [14C]-labeled tRNA established an incorporation ratio of three labeled carbons per tRNA molecule. Incorporation of the [14C]-label in vivo was confined to the methylation of nucleotides as determined by thin layer chromatography of nucleotides resulting from a ribonuclease digestion of [14C]-labeled tRNA. The carbon-13 NMR spectrum of [13C]-enriched tRNA indicated a similar degree of incorporation into the methylated nucleotides by the substantial enhancement of [13C]-methyl NMR signals only. Assignment of signals has been made for the methyl groups of ribothymidine and N7-methylguanosine in E. coli tRNA."}
{"id": "PMID:1101226", "title": "Misincorporation of (TP during transcription of poly dAT-dAT and poly dABU-dABU.", "content": "The synthetic DNA alternating copolymers poly dAT-dAT and dABU-dABU have been transcribed with E. coli RNA polymerase to measure the level of BrdU-induced misincorporation of guanine during transcription. GTP is found to be misincorporated into both copolymers at a frequency of 1 per 1000-2000 nucleotides polymerized. Using alpha-32P-GTP, the nearest neighbors to GMP are found to be UMP (approximately 63%), GMP (approximately 25%) and AMP (approximately 17%), with no apparent difference between the two templates. These results suggest that BrdU-substitution in DNA does not necessarily increase the potential for base mispairing during transcription, and hence, promote the production of faulty RNA molecules.", "contents": "Misincorporation of (TP during transcription of poly dAT-dAT and poly dABU-dABU. The synthetic DNA alternating copolymers poly dAT-dAT and dABU-dABU have been transcribed with E. coli RNA polymerase to measure the level of BrdU-induced misincorporation of guanine during transcription. GTP is found to be misincorporated into both copolymers at a frequency of 1 per 1000-2000 nucleotides polymerized. Using alpha-32P-GTP, the nearest neighbors to GMP are found to be UMP (approximately 63%), GMP (approximately 25%) and AMP (approximately 17%), with no apparent difference between the two templates. These results suggest that BrdU-substitution in DNA does not necessarily increase the potential for base mispairing during transcription, and hence, promote the production of faulty RNA molecules."}
{"id": "PMID:1101227", "title": "Analysis by isopycnic centrifugation of isolated nucleoids of Escherichia coli.", "content": "The isolated, formaldehyde-fixed nucleoid of E. coli has been analyzed by isopycnic centrifugation in CsCl density gradients. The membrane-free nucleoid bands at a density of 1.69 +/- 0.02 g/cm3. The membrane-associated nucleoid bands at a density of 1.46 +/- 0.02 g/cm3. Both species sediment to equilibrium as nearly monodisperse bands in CsCl, suggesting that the nucleoid components of DNA, RNA and protein are present in relatively constant ratios. These ratios are constant regardless of the position of the nucleoids in the heterogeneous sedimentation profile of a preparative sucrose gradient. The fixed nucleoids remain condensed during isopycnic centrifugation and there is no detectable loss of RNA from the nucleoid.", "contents": "Analysis by isopycnic centrifugation of isolated nucleoids of Escherichia coli. The isolated, formaldehyde-fixed nucleoid of E. coli has been analyzed by isopycnic centrifugation in CsCl density gradients. The membrane-free nucleoid bands at a density of 1.69 +/- 0.02 g/cm3. The membrane-associated nucleoid bands at a density of 1.46 +/- 0.02 g/cm3. Both species sediment to equilibrium as nearly monodisperse bands in CsCl, suggesting that the nucleoid components of DNA, RNA and protein are present in relatively constant ratios. These ratios are constant regardless of the position of the nucleoids in the heterogeneous sedimentation profile of a preparative sucrose gradient. The fixed nucleoids remain condensed during isopycnic centrifugation and there is no detectable loss of RNA from the nucleoid."}
{"id": "PMID:1101228", "title": "RNA nicking activity associated with DNA ligase of T4 infected E. coli: properties and influence on in vitro reactions of ligase.", "content": "Highly purified DNA ligase from T4 infected E. coli displays an RNA nicking activity which cleaves endonucleolytically the RNA of ribo-desoxy-and ribo-ribo type doublestranded structures to oligonucleotides with 5'phosphoryl-and 3'hydroxy termini. In the presence of ATP the generated nicks are repaired by the ligase except at the ends of the doublestranded regions where some short oligonucleotides are released before ligation can occur. As judged from its behaviour during the various purification steps and from some of its properties, the nicking activity seems to be different from known nicking enzymes.", "contents": "RNA nicking activity associated with DNA ligase of T4 infected E. coli: properties and influence on in vitro reactions of ligase. Highly purified DNA ligase from T4 infected E. coli displays an RNA nicking activity which cleaves endonucleolytically the RNA of ribo-desoxy-and ribo-ribo type doublestranded structures to oligonucleotides with 5'phosphoryl-and 3'hydroxy termini. In the presence of ATP the generated nicks are repaired by the ligase except at the ends of the doublestranded regions where some short oligonucleotides are released before ligation can occur. As judged from its behaviour during the various purification steps and from some of its properties, the nicking activity seems to be different from known nicking enzymes."}
{"id": "PMID:1101229", "title": "Atomic co-ordinates for yeast phenylalanine tRNA.", "content": "Atomic coordinates are presented for yeast tRNA(Phe) derived from a wire skeletal model fitted to an electron density map at 2.5 A resolution obtained by isomorphous replacement.", "contents": "Atomic co-ordinates for yeast phenylalanine tRNA. Atomic coordinates are presented for yeast tRNA(Phe) derived from a wire skeletal model fitted to an electron density map at 2.5 A resolution obtained by isomorphous replacement."}
{"id": "PMID:1101236", "title": "[Goblet cells in spontaneous colibacteriosis in pigs].", "content": "This work is a part of the studies on the intestinal barrier undertaken to cast some more light on the pathogenesis of colibacteriosis in pigs. The objective of the work was to investigate the goblet cells in the intestines of pigs ill with spontaneous colibacteriosis in the form of oedema disease and gastrointestinal form. The investigations were carried out on 26 pigs segregated into 3 groups: I group -- typical oedema disease (12 pigs), II group -- colibacteriosis in the gastrointestinal form (8 pigs), III group -- healthy controls (6 pigs). The segregation into the first two groups was based on the clinical, pathological and bacteriological criteria. In all the animals, the number of the goblet cells was counted in the duodenum, jejunum, ileum, cecum and colon. The results, analysed statistically, showed that in spontaneous colibacteriosis (oedema disease as well as the gastrointestinal form) a slight decrease of the number of the goblet cells was observed. In oedema disease, this decrease is significant only in the epithelium of the jejunal villi and in that of the colon, in the gastrointestinal form -- in the duodenal and jejunal villi. It results from the present investigations that the goblet cells, as one of the factors of the intestinal barrier, are not liable to such changes in spontaneous colibacteriosis as to explain or cast light on the pathogenesis of this disease.", "contents": "[Goblet cells in spontaneous colibacteriosis in pigs]. This work is a part of the studies on the intestinal barrier undertaken to cast some more light on the pathogenesis of colibacteriosis in pigs. The objective of the work was to investigate the goblet cells in the intestines of pigs ill with spontaneous colibacteriosis in the form of oedema disease and gastrointestinal form. The investigations were carried out on 26 pigs segregated into 3 groups: I group -- typical oedema disease (12 pigs), II group -- colibacteriosis in the gastrointestinal form (8 pigs), III group -- healthy controls (6 pigs). The segregation into the first two groups was based on the clinical, pathological and bacteriological criteria. In all the animals, the number of the goblet cells was counted in the duodenum, jejunum, ileum, cecum and colon. The results, analysed statistically, showed that in spontaneous colibacteriosis (oedema disease as well as the gastrointestinal form) a slight decrease of the number of the goblet cells was observed. In oedema disease, this decrease is significant only in the epithelium of the jejunal villi and in that of the colon, in the gastrointestinal form -- in the duodenal and jejunal villi. It results from the present investigations that the goblet cells, as one of the factors of the intestinal barrier, are not liable to such changes in spontaneous colibacteriosis as to explain or cast light on the pathogenesis of this disease."}
{"id": "PMID:1101237", "title": "[The nucleus cervicalis lateralis in sheep].", "content": "The objective of the work was to determine: a) the presence of the nucleus cervicalis lateralis in the sheep, b) the extent of this nucleus in the spinal cord and medulla oblongata, and c)the number and size of its cells. The histological method of staining the nerve tissue with methylene blue according to Nissl was employed. The nucleus cervicalis lateralis of the sheep is situated in the lateral funiculus, in the caudal part of the medulla oblongata and in the first three cervical segments of the spinal cord. The total number of cells making up the studied nucleus was 1629 in sheep I, 678 in sheep II, and 732 in sheep III. The average size of the cells ranged between 18.0 and 28.0 mu. In comparison with the same nucleus examined in other animals, that of the sheep shows a number of cell-free areas. It is composed of a very small number of cells, whose average diameter is small. These characteristics make it possible to regard the nucleus cervicalis lateralis in the sheep as a rudimentary structure.", "contents": "[The nucleus cervicalis lateralis in sheep]. The objective of the work was to determine: a) the presence of the nucleus cervicalis lateralis in the sheep, b) the extent of this nucleus in the spinal cord and medulla oblongata, and c)the number and size of its cells. The histological method of staining the nerve tissue with methylene blue according to Nissl was employed. The nucleus cervicalis lateralis of the sheep is situated in the lateral funiculus, in the caudal part of the medulla oblongata and in the first three cervical segments of the spinal cord. The total number of cells making up the studied nucleus was 1629 in sheep I, 678 in sheep II, and 732 in sheep III. The average size of the cells ranged between 18.0 and 28.0 mu. In comparison with the same nucleus examined in other animals, that of the sheep shows a number of cell-free areas. It is composed of a very small number of cells, whose average diameter is small. These characteristics make it possible to regard the nucleus cervicalis lateralis in the sheep as a rudimentary structure."}
{"id": "PMID:1101231", "title": "Pediatric surgical patients' and parents' stress responses and adjustment as a function of psychologic preparation and stress-point nursing care.", "content": "The purpose of this study was to test the hypotheses that children who receive systematic psychologic preparation and continued supportive care, in contrast to those who do not, would show less upset behavior and more cooperation in the hospital and fewer post-hospital adjustment problems and that their parents would be less anxious and more satisfied with information and care received. Eighty children scheduled for minor surgery and their parents were randomly assigned to experimental and control conditions. The experimental intervention consisted of accurate information about sequences of events, sensory experiences, role expectations and appropriate responses, previews of procedures through play techniques, and supportive care given at critical points pre- and postoperatively. Significant differences between experimental and control children and parents on ratings of upset behavior, cooperation with procedures, pulse before and after painful procedures, resistance to induction, time to first voiding, posthospital adjustment, and parental anxiety and satisfaction with information and care consistently supported the hypotheses. Results were also analyzed in relation to the age and sex of the children and whether parents roomed with the children.", "contents": "Pediatric surgical patients' and parents' stress responses and adjustment as a function of psychologic preparation and stress-point nursing care. The purpose of this study was to test the hypotheses that children who receive systematic psychologic preparation and continued supportive care, in contrast to those who do not, would show less upset behavior and more cooperation in the hospital and fewer post-hospital adjustment problems and that their parents would be less anxious and more satisfied with information and care received. Eighty children scheduled for minor surgery and their parents were randomly assigned to experimental and control conditions. The experimental intervention consisted of accurate information about sequences of events, sensory experiences, role expectations and appropriate responses, previews of procedures through play techniques, and supportive care given at critical points pre- and postoperatively. Significant differences between experimental and control children and parents on ratings of upset behavior, cooperation with procedures, pulse before and after painful procedures, resistance to induction, time to first voiding, posthospital adjustment, and parental anxiety and satisfaction with information and care consistently supported the hypotheses. Results were also analyzed in relation to the age and sex of the children and whether parents roomed with the children."}
{"id": "PMID:1101233", "title": "Analysis of nurse-doctorates; data collected for the International Directory of Nurses with Doctoral Degrees.", "content": "In an American Nurses' Foundation survey of the 1973 population of nurses with doctoral degrees (N = 1,020) general trends in doctoral education of nurses were elicited. Specific unique characteristics were highlighted, including: a continuing trend for nurses to earn the Ph.D. rather than the Ed.D., a divergence between field of doctoral study and the current field of research interest, and a slight number of these qualified nurses actually participating in research of any kind. The majority of subjects were single; most were employed in a university or college where they held either full or associate professorships; most considered themselves still to be in the field of nursing, although their doctoral studies frequently took them out of the discipline; their work responsibilities were heavily loaded with administrative duties. As compared with the holders of doctoral degrees in other fields, nurses earned their degrees later, but took no longer to complete them. While universities in the Mid-Atlantic states were the largest producers of nurse-doctorates, universities in the Midwest were found to be the most frequent employers. Questions regarding the future direction of nurse-doctorates were raised.", "contents": "Analysis of nurse-doctorates; data collected for the International Directory of Nurses with Doctoral Degrees. In an American Nurses' Foundation survey of the 1973 population of nurses with doctoral degrees (N = 1,020) general trends in doctoral education of nurses were elicited. Specific unique characteristics were highlighted, including: a continuing trend for nurses to earn the Ph.D. rather than the Ed.D., a divergence between field of doctoral study and the current field of research interest, and a slight number of these qualified nurses actually participating in research of any kind. The majority of subjects were single; most were employed in a university or college where they held either full or associate professorships; most considered themselves still to be in the field of nursing, although their doctoral studies frequently took them out of the discipline; their work responsibilities were heavily loaded with administrative duties. As compared with the holders of doctoral degrees in other fields, nurses earned their degrees later, but took no longer to complete them. While universities in the Mid-Atlantic states were the largest producers of nurse-doctorates, universities in the Midwest were found to be the most frequent employers. Questions regarding the future direction of nurse-doctorates were raised."}
{"id": "PMID:1101244", "title": "Reduction of airborne microorganisms by filtering recycled air in a chick hatcher.", "content": "An experimental chick hatcher designed to filter recycled ventilation air was tested for its effectiveness in reducing the number of viable airborne microorganisms. Chicks in a filtered hatcher and a control hatcher (no filter) were artificially contaminated with Serratia marcescens as ventilation air was recycled in the hatchers for twelve hours. The number of viable S. marcescens particles in the filtered air of the conditioning chamber was less than detectable. The number of viable airborne S. marcescens particles in the hatching chamber of the filtered hatcher indicated a reduction of greater than 90 percent over the number in the unfiltered hatcher. The filter was effective in reducing the number of airborne particles carrying viable S. marcescens organisms in the hatcher.", "contents": "Reduction of airborne microorganisms by filtering recycled air in a chick hatcher. An experimental chick hatcher designed to filter recycled ventilation air was tested for its effectiveness in reducing the number of viable airborne microorganisms. Chicks in a filtered hatcher and a control hatcher (no filter) were artificially contaminated with Serratia marcescens as ventilation air was recycled in the hatchers for twelve hours. The number of viable S. marcescens particles in the filtered air of the conditioning chamber was less than detectable. The number of viable airborne S. marcescens particles in the hatching chamber of the filtered hatcher indicated a reduction of greater than 90 percent over the number in the unfiltered hatcher. The filter was effective in reducing the number of airborne particles carrying viable S. marcescens organisms in the hatcher."}
{"id": "PMID:1101245", "title": "Effect of filtering recycled air in a chick hatcher on airborne pathogenic microorganisms.", "content": "Two experimental chick hatchers in which ventilation air within the hatcher was partially recirculated in a positive pressure system, one with an air filter in the system and the other without a filter, were simultaneously tested to determine effect of the filter on quantitative reduction of viable airborne microorganisms. Chicks were artificially contaminated with either Escherichia coli or Staphylococcus aureus (coagulase-positive). Air was sampled for total test bacteria per cubic foot of hatcher air. The filter effectively reduced the number of viable airborne particles contaminated with E. coli and S. aureus contributed by chicks in the hatcher.", "contents": "Effect of filtering recycled air in a chick hatcher on airborne pathogenic microorganisms. Two experimental chick hatchers in which ventilation air within the hatcher was partially recirculated in a positive pressure system, one with an air filter in the system and the other without a filter, were simultaneously tested to determine effect of the filter on quantitative reduction of viable airborne microorganisms. Chicks were artificially contaminated with either Escherichia coli or Staphylococcus aureus (coagulase-positive). Air was sampled for total test bacteria per cubic foot of hatcher air. The filter effectively reduced the number of viable airborne particles contaminated with E. coli and S. aureus contributed by chicks in the hatcher."}
{"id": "PMID:1101242", "title": "Release of a prostaglandin E-like substance into mixed venous blood during endotoxin hypotension in cats.", "content": "An iv injection of E. coli endotoxin (10--15 mg/kg) produced biphasic hypotensive reaction in cats. The second phase of hypotension developed 5--10 min after the administration of endotoxin and was paralleled by an appearance of a substance contracting the chick rectum and rat colon which were bathed in mixed venous blood. On the basis of differential sensitivity of the assay organs to prostaglandins it was concluded that a prostaglandin E-like substance (PGEs) was released. Its peak concentration in blood was 1--3-5 ng/ml in terms of PGE2-like activity. Indomethacin (10 mg/kg) interrupted the liberation of PGEs into blood and at the same time a slow but steady increase in arterial blood pressure occured. The amelioration of endotoxin hypotension by indomethacin was abolished by an infusion of PGE2 at a rate of 20--100 ng/kg/min. It is suggested that the second phase of endotoxin hypotension in cats is mediated by a release of PGEs.", "contents": "Release of a prostaglandin E-like substance into mixed venous blood during endotoxin hypotension in cats. An iv injection of E. coli endotoxin (10--15 mg/kg) produced biphasic hypotensive reaction in cats. The second phase of hypotension developed 5--10 min after the administration of endotoxin and was paralleled by an appearance of a substance contracting the chick rectum and rat colon which were bathed in mixed venous blood. On the basis of differential sensitivity of the assay organs to prostaglandins it was concluded that a prostaglandin E-like substance (PGEs) was released. Its peak concentration in blood was 1--3-5 ng/ml in terms of PGE2-like activity. Indomethacin (10 mg/kg) interrupted the liberation of PGEs into blood and at the same time a slow but steady increase in arterial blood pressure occured. The amelioration of endotoxin hypotension by indomethacin was abolished by an infusion of PGE2 at a rate of 20--100 ng/kg/min. It is suggested that the second phase of endotoxin hypotension in cats is mediated by a release of PGEs."}
{"id": "PMID:1101247", "title": "Idoxuridine in the treatment of herpes zoster.", "content": "An uncontrolled, double-blind, random-selection study of fifty consecutive patients with attacks of herpes zoster treated with one of two concentrations (5 per cent or 40 per cent) of idoxuridine (IDU) in dimethyl sulphoxide (DMSO) has shown that, over all, the patients fared better than would have been expected had they been treated only symptomatically. There was no apparent difference between the two concentrations of idoxuridine in regard to either side-effects or benefits. In 17 of the fifty patients the skin lesions healed more rapidly than would have been expected without treatment, and pain was relieved more rapidly than expected in 26 of the 47 patients in whom it was a feature of the attack. Side-effects, which included a transient stinging or burning sensation in 29 patients and acute sensitivity to idoxuridine (confirmed by patch-testing) in one, did not lead to withdrawal of any patient from the trial. Three patients complained of an unpleasant, garlicky taste during treatment. No significant abnormalities were noted in liver-function tests and in white-cell or platelet counts in patients in either treatment group. The solutions of idoxuridine in dimethyl sulphoxide were provided by W.B. Pharmaceutical Ltd.", "contents": "Idoxuridine in the treatment of herpes zoster. An uncontrolled, double-blind, random-selection study of fifty consecutive patients with attacks of herpes zoster treated with one of two concentrations (5 per cent or 40 per cent) of idoxuridine (IDU) in dimethyl sulphoxide (DMSO) has shown that, over all, the patients fared better than would have been expected had they been treated only symptomatically. There was no apparent difference between the two concentrations of idoxuridine in regard to either side-effects or benefits. In 17 of the fifty patients the skin lesions healed more rapidly than would have been expected without treatment, and pain was relieved more rapidly than expected in 26 of the 47 patients in whom it was a feature of the attack. Side-effects, which included a transient stinging or burning sensation in 29 patients and acute sensitivity to idoxuridine (confirmed by patch-testing) in one, did not lead to withdrawal of any patient from the trial. Three patients complained of an unpleasant, garlicky taste during treatment. No significant abnormalities were noted in liver-function tests and in white-cell or platelet counts in patients in either treatment group. The solutions of idoxuridine in dimethyl sulphoxide were provided by W.B. Pharmaceutical Ltd."}
{"id": "PMID:1101252", "title": "Granulation in chronic leg ulcers. A trial with a new material.", "content": "Twenty-two patients with chronic ulcers of the lower limb were treated with 'Varihesive', a modified form of 'Stomahesive'. The results showed that granulation was stimulated by the dressing, and no side-effects were encountered. The treatment is considered to be a useful adjunct to routine supportive measures. I should like to thank E.R. Squibb & Sons for providing the material for this study.", "contents": "Granulation in chronic leg ulcers. A trial with a new material. Twenty-two patients with chronic ulcers of the lower limb were treated with 'Varihesive', a modified form of 'Stomahesive'. The results showed that granulation was stimulated by the dressing, and no side-effects were encountered. The treatment is considered to be a useful adjunct to routine supportive measures. I should like to thank E.R. Squibb & Sons for providing the material for this study."}
{"id": "PMID:1101254", "title": "Isolation of DNA from yeast by chromatography on hydroxyapatite.", "content": "A simple procedure is described for isolation of purified non degraded total DNA from yeast cells. The procedure involves conversion of the cells into spheroplasts by enzymatic treatment, lysis of the spheroplasts in 8 M urea - 0.24 M sodium phosphate buffer - 0.01 M EDTA (ethylendiamintetraacetic acid, sodium salt) - 1% SDS (sodium dodecyl sulphate), deproteinization of the lysate with chloroform-phenol and separation of the DNA from proteins, RNA and other contaminants by hydroxyapatite chromatography. The yield is about 90% of the DNA in the starting material (spheroplasts).", "contents": "Isolation of DNA from yeast by chromatography on hydroxyapatite. A simple procedure is described for isolation of purified non degraded total DNA from yeast cells. The procedure involves conversion of the cells into spheroplasts by enzymatic treatment, lysis of the spheroplasts in 8 M urea - 0.24 M sodium phosphate buffer - 0.01 M EDTA (ethylendiamintetraacetic acid, sodium salt) - 1% SDS (sodium dodecyl sulphate), deproteinization of the lysate with chloroform-phenol and separation of the DNA from proteins, RNA and other contaminants by hydroxyapatite chromatography. The yield is about 90% of the DNA in the starting material (spheroplasts)."}
{"id": "PMID:1101255", "title": "Fragments of beta-galactosidase from Escherichia coli. Fragmentation, purification, characterization and in vitro complementation.", "content": "Thermal fragmentation of the beta-galactosidase was studied in different buffer solutions and at different temperatures. Fragmentation of the subunits in small size polypeptides could be observed directly. The fragmentation proceeded in buffer solution, pH 7.0, at either 75 degrees C or 100 degrees C in the presence of sodium dodecyl sulfate. The elevated temperature appeared to accelerate this process. At 100 degrees C, pH 7.2, the fragmentation proceeded in the absence of sodium dodecyl sulfate, but in the presence of 8 M urea. Molecular weights, determined by sodium dodecyl sulfate disc gel electrophoresis were from 130,000 to about 20,000. Multiple bands were observed. After dissociation was complete at 37 degrees C, the fragments were purified by ion-exchange column chromatography. Of the five fragments thus obtained, four were homogeneous by disc-gel electrophoresis. Molecular weight of the homogeneous fragments were found to be near 25,000. The fifth comprised a mixture of four fragments having molecular weights from 29,000 to 72,000. Two of the fragments were active as the alpha-donor in in vitro complementation with mutant M15, which contains a deletion in the alpha-region of the z gene.", "contents": "Fragments of beta-galactosidase from Escherichia coli. Fragmentation, purification, characterization and in vitro complementation. Thermal fragmentation of the beta-galactosidase was studied in different buffer solutions and at different temperatures. Fragmentation of the subunits in small size polypeptides could be observed directly. The fragmentation proceeded in buffer solution, pH 7.0, at either 75 degrees C or 100 degrees C in the presence of sodium dodecyl sulfate. The elevated temperature appeared to accelerate this process. At 100 degrees C, pH 7.2, the fragmentation proceeded in the absence of sodium dodecyl sulfate, but in the presence of 8 M urea. Molecular weights, determined by sodium dodecyl sulfate disc gel electrophoresis were from 130,000 to about 20,000. Multiple bands were observed. After dissociation was complete at 37 degrees C, the fragments were purified by ion-exchange column chromatography. Of the five fragments thus obtained, four were homogeneous by disc-gel electrophoresis. Molecular weight of the homogeneous fragments were found to be near 25,000. The fifth comprised a mixture of four fragments having molecular weights from 29,000 to 72,000. Two of the fragments were active as the alpha-donor in in vitro complementation with mutant M15, which contains a deletion in the alpha-region of the z gene."}
{"id": "PMID:1101258", "title": "In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase.", "content": "After infection with bacteriophage T7 the beta' and to a lesser extent the beta subunits of E. coli DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) are phosphorylated by a phage-gene-encoded protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). The phosphorylation occurs on threonine residues and appears site-specific. It is probably the molecular basis of the early transcriptional control.", "contents": "In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase. After infection with bacteriophage T7 the beta' and to a lesser extent the beta subunits of E. coli DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) are phosphorylated by a phage-gene-encoded protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). The phosphorylation occurs on threonine residues and appears site-specific. It is probably the molecular basis of the early transcriptional control."}
{"id": "PMID:1101259", "title": "Proton nuclear magnetic resonance of spin-labeled Escherichia coli tRNAf1MET.", "content": "Thiouridine at position 8 (s4U8) of tRNAf1Met was spin-labeled with the nitroxide free radical, N-(1-oxyl-2,2,5,5-Tetramethyl-3-pyrrolidinyl) bromacetamide, for proton nuclear magnetic resonance spectroscopic studies. The well-resolved methyl peak of ribothymidine is unperturbed, but the peak tentatively assigned to the C-5 methylene group of dihydrouridine is considerably broadened in spin-labeled tRNAf1Met. Of the approximately 27 slowly exchanging protons observed in the region between 11 and 15 ppm downfield from 4,4-dimethyl-4-silapentane-1-sulfonic acid, the equivalent of about five protons apparently disappeared in spin-labeled tRNAf1Met. The well-resolved single proton at 14.8 ppm was missing not only in the paramagnetic species, but also in the diamagnetic reduced form of spin-labeled tRNAf1Met, and was unequivocally identified as a hydrogen bond involving s4U8 by comparison of several forms of tRNAf1Met specifically modified at s4U. Evidence that the perturbation of a second single proton resonance at 14.6 ppm (shift and broadening) is coupled to the loss of a tertiary hydrogen bond involving residue 8, arises from the same modified forms. The resolved resonances in the methyl and N-H regions, particularly the resonance at 14.6 ppm as well as the four N-bonded proton resonances at higher field which are broadened solely due to their proximity to the unpaired electron of the spin label, provide specific indicators of the geometry of tRNAf1Met structure in solution. Their observability by nuclear magnetic resonance spectroscopy opens up the possibility of monitoring distance changes among the base residues of spin-labeled tRNAf1Met upon its interaction with aminoacyl-tRNA synthetase and other enzymes.", "contents": "Proton nuclear magnetic resonance of spin-labeled Escherichia coli tRNAf1MET. Thiouridine at position 8 (s4U8) of tRNAf1Met was spin-labeled with the nitroxide free radical, N-(1-oxyl-2,2,5,5-Tetramethyl-3-pyrrolidinyl) bromacetamide, for proton nuclear magnetic resonance spectroscopic studies. The well-resolved methyl peak of ribothymidine is unperturbed, but the peak tentatively assigned to the C-5 methylene group of dihydrouridine is considerably broadened in spin-labeled tRNAf1Met. Of the approximately 27 slowly exchanging protons observed in the region between 11 and 15 ppm downfield from 4,4-dimethyl-4-silapentane-1-sulfonic acid, the equivalent of about five protons apparently disappeared in spin-labeled tRNAf1Met. The well-resolved single proton at 14.8 ppm was missing not only in the paramagnetic species, but also in the diamagnetic reduced form of spin-labeled tRNAf1Met, and was unequivocally identified as a hydrogen bond involving s4U8 by comparison of several forms of tRNAf1Met specifically modified at s4U. Evidence that the perturbation of a second single proton resonance at 14.6 ppm (shift and broadening) is coupled to the loss of a tertiary hydrogen bond involving residue 8, arises from the same modified forms. The resolved resonances in the methyl and N-H regions, particularly the resonance at 14.6 ppm as well as the four N-bonded proton resonances at higher field which are broadened solely due to their proximity to the unpaired electron of the spin label, provide specific indicators of the geometry of tRNAf1Met structure in solution. Their observability by nuclear magnetic resonance spectroscopy opens up the possibility of monitoring distance changes among the base residues of spin-labeled tRNAf1Met upon its interaction with aminoacyl-tRNA synthetase and other enzymes."}
{"id": "PMID:1101260", "title": "Sulfonamide resistance mechanism in Escherichia coli: R plasmids can determine sulfonamide-resistant dihydropteroate synthases.", "content": "Several natural isolate E. coli strains highly resistant to sulfonamides and antibiotics are shown to contain a sulfonamide-resistant dihydropteroate synthase (2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine-diphosphate:4-aminobenzoate 2-amino-4-hydroxydihydropteridine-6-methenyltransferase, EC 2.5.1.15) in addition to the normal sensitive enzyme. The resistant dihydropteroate synthases examined are determined by an R plasmid and are smaller and less heat stable than the normal sulfonamide-sensitive enzyme. One synthase resistant to any sulfonamide tested, and to sulfanilic and arsanilic acids, was still inhibited by several non-sulfonamide analogs of p-aminobenzoate. Citrobacter and Klebsiella pneumoniae strains also show similar mechanisms of sulfonamide resistance.", "contents": "Sulfonamide resistance mechanism in Escherichia coli: R plasmids can determine sulfonamide-resistant dihydropteroate synthases. Several natural isolate E. coli strains highly resistant to sulfonamides and antibiotics are shown to contain a sulfonamide-resistant dihydropteroate synthase (2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine-diphosphate:4-aminobenzoate 2-amino-4-hydroxydihydropteridine-6-methenyltransferase, EC 2.5.1.15) in addition to the normal sensitive enzyme. The resistant dihydropteroate synthases examined are determined by an R plasmid and are smaller and less heat stable than the normal sulfonamide-sensitive enzyme. One synthase resistant to any sulfonamide tested, and to sulfanilic and arsanilic acids, was still inhibited by several non-sulfonamide analogs of p-aminobenzoate. Citrobacter and Klebsiella pneumoniae strains also show similar mechanisms of sulfonamide resistance."}
{"id": "PMID:1101261", "title": "Release of (oligo) peptidyl-tRNA from ribosomes by erythromycin A.", "content": "Erythromycin A released peptidyl-tRNA in the in vitro polypeptide synthesis system with bacterial components programmed by synthetic polynucleotide. This is consistent with our hypothesis that erythromycin A inhibits translocation by preventing proper situation of oligopeptidyl-tRNA in the donor (D) site on ribosomes.", "contents": "Release of (oligo) peptidyl-tRNA from ribosomes by erythromycin A. Erythromycin A released peptidyl-tRNA in the in vitro polypeptide synthesis system with bacterial components programmed by synthetic polynucleotide. This is consistent with our hypothesis that erythromycin A inhibits translocation by preventing proper situation of oligopeptidyl-tRNA in the donor (D) site on ribosomes."}
{"id": "PMID:1101262", "title": "Origin of hemopoietic stem cells in embryonic bursa of Fabricius and bone marrow studied through interspecific chimeras.", "content": "The histogenesis of the bursa of Fabricius and of bone marrow was studied by a biological cell marking technique based on differences in the nuclear structure of two species of birds, Japanese quail (Coturnix coturnix japonica) and chick (Gallus gallus). In quail cells the nucleus contains a large amount of heterochromatin associated with the nucleolus. That makes it possible to distinguish them from chick cells after Feulgen-Rossenbeck staining and by electron microscopy. By grafting bursal rudiments and limb buds of quail into chick and inversely it was possible to demonstrate that the whole hemopoietic population of the bursa of Fabricius and of bone marrow is derived from bloodborne extrinsic stem cells. Neither endoderm nor mesoderm of the bursal rudiments is capable of differentiating into lymphoid cells. Combinations of quail bursal endoderm with chick homologous mesenchyme showed that the reticular cells of the follicles are the only endodermal derivatives of the bursa. The mesenchymal bursal component gives rise to the interfollicular connective cells. The contribution to bone marrow histogenesis of cells of vascular and blood origin, on one hand, and of the elements of the cartilaginous model, on the other hand, was analyzed. It appeared that osteoblasts, osteocytes, and stromal cells of marrow are derived from the perichondrium. In contrast, the endothelium of the vascular buds and the hemopoietic cells which invade the diaphysal cartilage during the endochondral ossification process do not belong to the mesenchymal bone primordium but have a fully extrinsic origin.", "contents": "Origin of hemopoietic stem cells in embryonic bursa of Fabricius and bone marrow studied through interspecific chimeras. The histogenesis of the bursa of Fabricius and of bone marrow was studied by a biological cell marking technique based on differences in the nuclear structure of two species of birds, Japanese quail (Coturnix coturnix japonica) and chick (Gallus gallus). In quail cells the nucleus contains a large amount of heterochromatin associated with the nucleolus. That makes it possible to distinguish them from chick cells after Feulgen-Rossenbeck staining and by electron microscopy. By grafting bursal rudiments and limb buds of quail into chick and inversely it was possible to demonstrate that the whole hemopoietic population of the bursa of Fabricius and of bone marrow is derived from bloodborne extrinsic stem cells. Neither endoderm nor mesoderm of the bursal rudiments is capable of differentiating into lymphoid cells. Combinations of quail bursal endoderm with chick homologous mesenchyme showed that the reticular cells of the follicles are the only endodermal derivatives of the bursa. The mesenchymal bursal component gives rise to the interfollicular connective cells. The contribution to bone marrow histogenesis of cells of vascular and blood origin, on one hand, and of the elements of the cartilaginous model, on the other hand, was analyzed. It appeared that osteoblasts, osteocytes, and stromal cells of marrow are derived from the perichondrium. In contrast, the endothelium of the vascular buds and the hemopoietic cells which invade the diaphysal cartilage during the endochondral ossification process do not belong to the mesenchymal bone primordium but have a fully extrinsic origin."}
{"id": "PMID:1101263", "title": "Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.", "content": "An analysis of revertants of missense mutants in phage P22 has shown: (i) New temperature-sensitive (TS) and cold-sensitive (CS) phenotypes are often acquired concomitant with reversion. (ii) In many cases, these new phenotypes are due to second-site mutations (suppressors) that correct the original defect. (iii) Sometimes the suppressor mutation is not in the same gene as the original mutation. (iv) Extragenic suppressors are almost always in genes whose products are known to interact physically with the original gene products. (v) The suppressor mutations typically retain their TS or CS phenotypes when crossed into wild-type genetic backgrounds. (vi) Some TS and CS mutants derived by reversion can themselves be reverted to produce additional mutations. We have shown that genetic reversion of missense mutants can be of value in producing new temperature-sensitive and cold-sensitive mutations affecting related functions. We suggest that our approach can be extended to organisms with large genomes.", "contents": "Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins. An analysis of revertants of missense mutants in phage P22 has shown: (i) New temperature-sensitive (TS) and cold-sensitive (CS) phenotypes are often acquired concomitant with reversion. (ii) In many cases, these new phenotypes are due to second-site mutations (suppressors) that correct the original defect. (iii) Sometimes the suppressor mutation is not in the same gene as the original mutation. (iv) Extragenic suppressors are almost always in genes whose products are known to interact physically with the original gene products. (v) The suppressor mutations typically retain their TS or CS phenotypes when crossed into wild-type genetic backgrounds. (vi) Some TS and CS mutants derived by reversion can themselves be reverted to produce additional mutations. We have shown that genetic reversion of missense mutants can be of value in producing new temperature-sensitive and cold-sensitive mutations affecting related functions. We suggest that our approach can be extended to organisms with large genomes."}
{"id": "PMID:1101264", "title": "Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.", "content": "The transducing phage lambdarifd18 isolated by Kirschbaum and Konrad [(1973 J. Bacteriol. 116, 517-526] was found to carry structural genes for several 50S ribosomal proteins and 16S and 23S rRNA. It has previously been demonstrated [Kirschbaum & Scaife (1974) Mol. Gen. Genet. 132, 193-201] that this phage carries genes for the DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) subunits beta and beta'. Thus, the region of the E. coli chromosome carried by lambdarifd18 contains a cluster of genes essential for transcription and translation.", "contents": "Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits. The transducing phage lambdarifd18 isolated by Kirschbaum and Konrad [(1973 J. Bacteriol. 116, 517-526] was found to carry structural genes for several 50S ribosomal proteins and 16S and 23S rRNA. It has previously been demonstrated [Kirschbaum & Scaife (1974) Mol. Gen. Genet. 132, 193-201] that this phage carries genes for the DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) subunits beta and beta'. Thus, the region of the E. coli chromosome carried by lambdarifd18 contains a cluster of genes essential for transcription and translation."}
{"id": "PMID:1101265", "title": "Inducible error-prone repair in Escherichia coli.", "content": "A hypothesis that ultraviolet-induced mutagenesis arises from the induction of an error-prone mode of postreplication repair that requires the exrA+ recA+ genotype has been tested with alkaline sucrose gradient centrifugation coupled with assays of fixation determined by loss of photoreversibility. The inhibitor of protein synthesis, chloramphenicol, added before irradiation, prevented a small amount of postreplication repair and completely eliminated mutation fixation in E. coli WP2s uvrA. However, chloramphenicol did not affect strand joining: (a) in uvrA bacteria allowed 20 min of growth between irradiation and antibiotic treatment; (b) in nonmutable uvrA exrA bacteria; and (c) in uvrA tif bacteria grown at 42 degrees for 70 min before irradiation. These observations indicate that an inducible product is involved in a fraction of postreplication repair and is responsible for induced mutagenesis.", "contents": "Inducible error-prone repair in Escherichia coli. A hypothesis that ultraviolet-induced mutagenesis arises from the induction of an error-prone mode of postreplication repair that requires the exrA+ recA+ genotype has been tested with alkaline sucrose gradient centrifugation coupled with assays of fixation determined by loss of photoreversibility. The inhibitor of protein synthesis, chloramphenicol, added before irradiation, prevented a small amount of postreplication repair and completely eliminated mutation fixation in E. coli WP2s uvrA. However, chloramphenicol did not affect strand joining: (a) in uvrA bacteria allowed 20 min of growth between irradiation and antibiotic treatment; (b) in nonmutable uvrA exrA bacteria; and (c) in uvrA tif bacteria grown at 42 degrees for 70 min before irradiation. These observations indicate that an inducible product is involved in a fraction of postreplication repair and is responsible for induced mutagenesis."}
{"id": "PMID:1101266", "title": "New chromosomal location for structural genes of ribosomal proteins.", "content": "An Escherichia coli mutant, ts9, previously reported by Flaks et al. (Cold Spring Harbor Symp. Quant. Biol. 31, 623-631, 1966) to have an electrophoretically altered ribosomal protein, has been further characterized and the altered component has been identified as L7/L12. Although mutant ts9 is temperature sensitive for growth (rts-), the rts and L7/L12 mutations are genetically separable and are both located between argH and rif. The L7/L12 mutation (rpyL) maps very close to relC, mutants of which have a defect in the 50S ribosomal subunit. The gene order is argH-rts-(rpyL,relC)-rif. Protein synthesis directed by bacteriophage lambdacI857S7drifd18 in ultraviolet-irradiated cells indicates that L7/L12, As well as L1, L10, L11, and possibly L8 or L9 are coded by the phage DNA. Our results indicate that rpyL is the structural gene for L7/L12 and that this region of the E. coli chromosome contains a cluster of structural genes for ribosomal proteins.", "contents": "New chromosomal location for structural genes of ribosomal proteins. An Escherichia coli mutant, ts9, previously reported by Flaks et al. (Cold Spring Harbor Symp. Quant. Biol. 31, 623-631, 1966) to have an electrophoretically altered ribosomal protein, has been further characterized and the altered component has been identified as L7/L12. Although mutant ts9 is temperature sensitive for growth (rts-), the rts and L7/L12 mutations are genetically separable and are both located between argH and rif. The L7/L12 mutation (rpyL) maps very close to relC, mutants of which have a defect in the 50S ribosomal subunit. The gene order is argH-rts-(rpyL,relC)-rif. Protein synthesis directed by bacteriophage lambdacI857S7drifd18 in ultraviolet-irradiated cells indicates that L7/L12, As well as L1, L10, L11, and possibly L8 or L9 are coded by the phage DNA. Our results indicate that rpyL is the structural gene for L7/L12 and that this region of the E. coli chromosome contains a cluster of structural genes for ribosomal proteins."}
{"id": "PMID:1101267", "title": "A bacterial mutation blocking P2 phage late gene expression.", "content": "A mutant of Escherichia coli strain C has been isolated, called gro109, that blocks bacteriophage P2 propagation by interfering with late gene expression. DNA replication proceeds normally in P2+-infected gro109 cells, but late phage proteins are not made. Early P2 mRNA is made in normal amounts, but very little late mRNA can be detected. P2 mutants (P2 ogr) able to overcome the gro109 block have been isolated in which synthesis of late P2 mRNA and phage proteins Is restored. The gro109 mutation is closely linked to the cluster of ribosomal genes at 64 min and is recessive to the wild-type (gro+) allele. A P2 ogr mutation has been mapped on the left arm of the P2 genome, between the right-most known late gene (D) and the phage attachment site. P2 ogr can complement P2+ in gro109 cells, indicating that ogr codes for a diffusible product.", "contents": "A bacterial mutation blocking P2 phage late gene expression. A mutant of Escherichia coli strain C has been isolated, called gro109, that blocks bacteriophage P2 propagation by interfering with late gene expression. DNA replication proceeds normally in P2+-infected gro109 cells, but late phage proteins are not made. Early P2 mRNA is made in normal amounts, but very little late mRNA can be detected. P2 mutants (P2 ogr) able to overcome the gro109 block have been isolated in which synthesis of late P2 mRNA and phage proteins Is restored. The gro109 mutation is closely linked to the cluster of ribosomal genes at 64 min and is recessive to the wild-type (gro+) allele. A P2 ogr mutation has been mapped on the left arm of the P2 genome, between the right-most known late gene (D) and the phage attachment site. P2 ogr can complement P2+ in gro109 cells, indicating that ogr codes for a diffusible product."}
{"id": "PMID:1101268", "title": "Somnolence, akinesia, and sensory activation of motivated behavior in the lateral hypothalamic syndrome.", "content": "After lateral hypothalamic damage in rats, somnolence, akinesia, and sensory neglect combine to produce complete aphagia. Only simple automatisms (such as grooming, chewing, licking) are present, but intense stimuli can activate more complex actions (walking, orientation, swimming). In the anorexic stage, tactile stimuli dominate in steering locomotion and \"spontaneous\" locomotion depends on activation from the empty stomach.", "contents": "Somnolence, akinesia, and sensory activation of motivated behavior in the lateral hypothalamic syndrome. After lateral hypothalamic damage in rats, somnolence, akinesia, and sensory neglect combine to produce complete aphagia. Only simple automatisms (such as grooming, chewing, licking) are present, but intense stimuli can activate more complex actions (walking, orientation, swimming). In the anorexic stage, tactile stimuli dominate in steering locomotion and \"spontaneous\" locomotion depends on activation from the empty stomach."}
{"id": "PMID:1101271", "title": "Increased glycolysis in immature rat ovaries using endogenous gonadotropins secreted in response to exogenous luteinizing hormone-releasing hormone.", "content": "The rate of glycolysis, as indicated by in vitro lactic acid production, in ovaries from immature rats was stimulated by raising serum concentrations of endogenous LH and FSH by intravenous injections of luteinizing hormone-releasing hormone. The rate was shown to be a function of the serum concentration of gonadotropin but the response required the presence of elevated hormone levels for about 1 hr. These data are offered as further evidence for the lack of physiological importance of brief changes in pituitary function.", "contents": "Increased glycolysis in immature rat ovaries using endogenous gonadotropins secreted in response to exogenous luteinizing hormone-releasing hormone. The rate of glycolysis, as indicated by in vitro lactic acid production, in ovaries from immature rats was stimulated by raising serum concentrations of endogenous LH and FSH by intravenous injections of luteinizing hormone-releasing hormone. The rate was shown to be a function of the serum concentration of gonadotropin but the response required the presence of elevated hormone levels for about 1 hr. These data are offered as further evidence for the lack of physiological importance of brief changes in pituitary function."}
{"id": "PMID:1101284", "title": "Cryoglobulins. III. Further studies on the nature, incidence, clinical, diagnostic, prognostic, and immunopathologic significance of cryoproteins in renal disease.", "content": "Serial serum samples from a large number of patients with immunologic renal disease, normal healthy controls, acute infections as well as non-immunological renal disease were studied for the presence, nature and properties of cryoproteins, and these correlated with serial renal functional, morphologic, immunohistologic and clinical fingings as well as serologic observations. A high incidence of cryoproteins were found in renal disease thought to be mediated by immune complexes. Cryoprecipitates were not detected in the other patients. The presence of fibrinogen in a serum cryoprecipitate was always associated with rapidly progressive disease and poor prognosis. An association between the detection of cryoproteins and the clinical and morphological activity of disease was observed. Persistence of cryoproteinemia was associated with progression and apparent disappearance with resolution or progression to end stage renal disease. In patients with hematuria or proteinuria of questionable significance cryoprotein detection was always associated with immune complex nephritis. Renal transplantation in the presence of cryoproteinemia was associated with recurrent nephritis in the graft. Cryoproteins were found to have biologic properties attributable to antigen-antibody complexes, to contain immune complexes of antigen and antibody and to have serologic factors concentrated. The detection of serum cryoglobulins was found to be a better index of clinical and morphologic activity of immune complex renal disease than was serum complement. As in our previous studies, these proteins appear to be of diagnostic and prognostic value in renal disease and provide a method of antigen identification in these disorders.", "contents": "Cryoglobulins. III. Further studies on the nature, incidence, clinical, diagnostic, prognostic, and immunopathologic significance of cryoproteins in renal disease. Serial serum samples from a large number of patients with immunologic renal disease, normal healthy controls, acute infections as well as non-immunological renal disease were studied for the presence, nature and properties of cryoproteins, and these correlated with serial renal functional, morphologic, immunohistologic and clinical fingings as well as serologic observations. A high incidence of cryoproteins were found in renal disease thought to be mediated by immune complexes. Cryoprecipitates were not detected in the other patients. The presence of fibrinogen in a serum cryoprecipitate was always associated with rapidly progressive disease and poor prognosis. An association between the detection of cryoproteins and the clinical and morphological activity of disease was observed. Persistence of cryoproteinemia was associated with progression and apparent disappearance with resolution or progression to end stage renal disease. In patients with hematuria or proteinuria of questionable significance cryoprotein detection was always associated with immune complex nephritis. Renal transplantation in the presence of cryoproteinemia was associated with recurrent nephritis in the graft. Cryoproteins were found to have biologic properties attributable to antigen-antibody complexes, to contain immune complexes of antigen and antibody and to have serologic factors concentrated. The detection of serum cryoglobulins was found to be a better index of clinical and morphologic activity of immune complex renal disease than was serum complement. As in our previous studies, these proteins appear to be of diagnostic and prognostic value in renal disease and provide a method of antigen identification in these disorders."}
{"id": "PMID:1101285", "title": "Bronchial asthma in the Nigerian savanna region. A clinical and laboratory study of 106 patients with a review of the literature on asthma in the tropics.", "content": "One hundred and six asthma patients were studied in Zaria in the Nigerian savanna region. This group resembled hospital attenders in general in containing a disproportionately large number of immigrants from southern Nigeria and students undergoing higher education. Childhood asthma was rare. Asthma started after the age of 19 years in 69 per cent of patients. Twenty-seven per cent gave a history of rhinitis but none had had eczema. Twenty-two per cent gave a family history of asthma. Cutaneous hypersensitivity to house dust supported by a history of attacks being precipitated by dust was found in 41 per cent of patients. Asthma was worst in the rainy season in 45 per cent of patients. Mites were found in mattress dust samples; the mean count was 243 mites per g dust; Dermatophagoides farinae formed 86-6 per cent of the total mite population. The variability of airways obstruction averaged 50 per cent of maximum values for forced expiratory volume in the first second (FEV1) and peak expiratory flow (PEF). The median severity of airways obstruction measured as FEV1/VC per cent was four standard deviations below predicted normal. Eighty-seven per cent of patients were positive to prick skin tests with one or more allergens. The commonest reactions were to house dust (58 per cent), house dust mite (45 per cent) and Dermatophagoides farinae (44 per cent). Fifty-one per cent of a group of controls were also positive on skin testing but the pattern of responses was different from the asthmatic patients. This high proportion of reactors is explained by high allergen load. Serum IgE levels were lower in the asthmatics than in a group of healthy controls who showed the very high levels characteristic of some African populations. We suggest that the controls were protected from atopic disease by developing high blocking levels of non-specific IgE, perhaps in response to gut helminths. The clinical pattern of asthma in Zaria is compared with other countries in the tropical and temperate zones. The particular problems of treating asthma in developing tropical countries are discussed.", "contents": "Bronchial asthma in the Nigerian savanna region. A clinical and laboratory study of 106 patients with a review of the literature on asthma in the tropics. One hundred and six asthma patients were studied in Zaria in the Nigerian savanna region. This group resembled hospital attenders in general in containing a disproportionately large number of immigrants from southern Nigeria and students undergoing higher education. Childhood asthma was rare. Asthma started after the age of 19 years in 69 per cent of patients. Twenty-seven per cent gave a history of rhinitis but none had had eczema. Twenty-two per cent gave a family history of asthma. Cutaneous hypersensitivity to house dust supported by a history of attacks being precipitated by dust was found in 41 per cent of patients. Asthma was worst in the rainy season in 45 per cent of patients. Mites were found in mattress dust samples; the mean count was 243 mites per g dust; Dermatophagoides farinae formed 86-6 per cent of the total mite population. The variability of airways obstruction averaged 50 per cent of maximum values for forced expiratory volume in the first second (FEV1) and peak expiratory flow (PEF). The median severity of airways obstruction measured as FEV1/VC per cent was four standard deviations below predicted normal. Eighty-seven per cent of patients were positive to prick skin tests with one or more allergens. The commonest reactions were to house dust (58 per cent), house dust mite (45 per cent) and Dermatophagoides farinae (44 per cent). Fifty-one per cent of a group of controls were also positive on skin testing but the pattern of responses was different from the asthmatic patients. This high proportion of reactors is explained by high allergen load. Serum IgE levels were lower in the asthmatics than in a group of healthy controls who showed the very high levels characteristic of some African populations. We suggest that the controls were protected from atopic disease by developing high blocking levels of non-specific IgE, perhaps in response to gut helminths. The clinical pattern of asthma in Zaria is compared with other countries in the tropical and temperate zones. The particular problems of treating asthma in developing tropical countries are discussed."}
{"id": "PMID:1101286", "title": "Treatment of Paget's disease of bone with porcine calcitonin: clinical and metabolic responses.", "content": "The clinical and metabolic responses of 12 patients with painful Paget's disease have been assessed before, during and after an 18-day course of treatment with purified porcine calcitonin, 160 MRC units i.m. daily. The indices of response included relief from pain, changes in concentration of serum alkaline phosphatase, calcium, magnesium, electrolytes and plasma inorganic phosphate; balance studies of calcium, phosphate, magnesium and in some cases sodium and potassium were undertaken. Urinary excretion of hydroxyproline was also measured. Remission of pain was complete in 11 of 15 courses of treatment. Coexistent osteoarthrosis was considered to be responsible where remission was incomplete. The mean duration of remission of pain was 12 months. Three patients who received a second course of treatment achieved further remission of pain. Side effects were not troublesome. Serum levels of alkaline phosphatase decreased to 67 per cent of the initial reading and the degree of suppression depended on the initial alkaline phosphatase activity. Urinary hydroxyproline correlated closely with the activity of serum alkaline phosphatase before, during and after treatment. The ratio of serum alkaline phosphatase:hydroxyproline excretion rose at the start and at the termination of treatment. Retention of calcium and inorganic phosphate occurred during the early phase of treatment only. Withdrawal of treatment resulted in a rebound retention of calcium and inorganic phosphate. The degree of calcium retention after treatment correlated with the level of plasma inorganic phosphate prevailing before treatment. The efficacy of these short courses of calcitonin are discussed and the metabolic observations related to theories of bone remodelling at a cellular and hormonal level.", "contents": "Treatment of Paget's disease of bone with porcine calcitonin: clinical and metabolic responses. The clinical and metabolic responses of 12 patients with painful Paget's disease have been assessed before, during and after an 18-day course of treatment with purified porcine calcitonin, 160 MRC units i.m. daily. The indices of response included relief from pain, changes in concentration of serum alkaline phosphatase, calcium, magnesium, electrolytes and plasma inorganic phosphate; balance studies of calcium, phosphate, magnesium and in some cases sodium and potassium were undertaken. Urinary excretion of hydroxyproline was also measured. Remission of pain was complete in 11 of 15 courses of treatment. Coexistent osteoarthrosis was considered to be responsible where remission was incomplete. The mean duration of remission of pain was 12 months. Three patients who received a second course of treatment achieved further remission of pain. Side effects were not troublesome. Serum levels of alkaline phosphatase decreased to 67 per cent of the initial reading and the degree of suppression depended on the initial alkaline phosphatase activity. Urinary hydroxyproline correlated closely with the activity of serum alkaline phosphatase before, during and after treatment. The ratio of serum alkaline phosphatase:hydroxyproline excretion rose at the start and at the termination of treatment. Retention of calcium and inorganic phosphate occurred during the early phase of treatment only. Withdrawal of treatment resulted in a rebound retention of calcium and inorganic phosphate. The degree of calcium retention after treatment correlated with the level of plasma inorganic phosphate prevailing before treatment. The efficacy of these short courses of calcitonin are discussed and the metabolic observations related to theories of bone remodelling at a cellular and hormonal level."}
{"id": "PMID:1101287", "title": "Avascular necrosis of bone following renal transplantation.", "content": "Assessment of 78 successful renal transplants carried out in Newcastle upon Tyne between October 1967 and March 1973, revealed avascular necrosis of bone (A.N.) in 11 patients (14 percent). Pain and restriction of movement were the cardinal symptoms, while the onset was from five to 35 months after transplantation. In some patients one joint only was affected but in seven patients (64 per cent) A.N. developed in other joints too in an additive and progressive fashion. A time lag was usually noted between the onset of symptoms and radiological proof. These 11 patients with A.N. were compared with two control groups of unaffected renal transplant patients. Taking into account the patients' body weight, the cumulative dose of prednisone received by affected patients during the first three post-transplant months was found to be significantly higher than that for both control groups (P less than 0.05). Patients with A.N. were found to have had an unusually high incidence of early post-transplant surgical complications and the length of their initial stay in hospital was significantly prolonged (P less than 0.02) when compared to both control groups. It is possible that excessive corticosteroid therapy is more deleterious in transplant patients weakened by peri-renal haematomas, urinary extravasations and stormy prolonged post-operative periods. Having emphasized the detrimental effects of excessive anti-rejection corticosteroid therapy it must be mentioned that occasionally patients develop avascular necrosis even after low doses of steroid therapy, while others, receiving high doses of steroid therapy and undergoing a stormy post-operative course, do not develop this complaint. It is suggested that although excessive steroid therapy is positively harmful, avascular necrosis is not an invariable complication and that ofther factors including possibly a genetic predisposition operate to produce avascular necrosis of bone in renal transplant patients.", "contents": "Avascular necrosis of bone following renal transplantation. Assessment of 78 successful renal transplants carried out in Newcastle upon Tyne between October 1967 and March 1973, revealed avascular necrosis of bone (A.N.) in 11 patients (14 percent). Pain and restriction of movement were the cardinal symptoms, while the onset was from five to 35 months after transplantation. In some patients one joint only was affected but in seven patients (64 per cent) A.N. developed in other joints too in an additive and progressive fashion. A time lag was usually noted between the onset of symptoms and radiological proof. These 11 patients with A.N. were compared with two control groups of unaffected renal transplant patients. Taking into account the patients' body weight, the cumulative dose of prednisone received by affected patients during the first three post-transplant months was found to be significantly higher than that for both control groups (P less than 0.05). Patients with A.N. were found to have had an unusually high incidence of early post-transplant surgical complications and the length of their initial stay in hospital was significantly prolonged (P less than 0.02) when compared to both control groups. It is possible that excessive corticosteroid therapy is more deleterious in transplant patients weakened by peri-renal haematomas, urinary extravasations and stormy prolonged post-operative periods. Having emphasized the detrimental effects of excessive anti-rejection corticosteroid therapy it must be mentioned that occasionally patients develop avascular necrosis even after low doses of steroid therapy, while others, receiving high doses of steroid therapy and undergoing a stormy post-operative course, do not develop this complaint. It is suggested that although excessive steroid therapy is positively harmful, avascular necrosis is not an invariable complication and that ofther factors including possibly a genetic predisposition operate to produce avascular necrosis of bone in renal transplant patients."}
{"id": "PMID:1101288", "title": "Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle.", "content": "Mathematical models for the analysis of pulse-cytophotometric (PCP) data are described. With computer programs based on these models the fractions of cells in G1-, S- and (G2 + M)-phase are obtained. The methods are applied to PCP-measurements of Ehrlich ascites tumor cells, human bone marrow cells and L-929-cells in culture. The results of the L-cell experiment are compared with autoradiographic results; for both methods the duration of the various phases has been calculated. Two different mathematical models for PCP-data evaluation and the autoradiographic method yielded results agreeing within statistical error. The application of the two models on different types of DNA-histograms is discussed: One model is suitable for asynchronous cell populations with a low fraction of S-phase cells, the other can be applied for partially synchronized cells and high S-phase fractions as well.", "contents": "Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle. Mathematical models for the analysis of pulse-cytophotometric (PCP) data are described. With computer programs based on these models the fractions of cells in G1-, S- and (G2 + M)-phase are obtained. The methods are applied to PCP-measurements of Ehrlich ascites tumor cells, human bone marrow cells and L-929-cells in culture. The results of the L-cell experiment are compared with autoradiographic results; for both methods the duration of the various phases has been calculated. Two different mathematical models for PCP-data evaluation and the autoradiographic method yielded results agreeing within statistical error. The application of the two models on different types of DNA-histograms is discussed: One model is suitable for asynchronous cell populations with a low fraction of S-phase cells, the other can be applied for partially synchronized cells and high S-phase fractions as well."}
{"id": "PMID:1101289", "title": "Biological damage from the Auger effect, possible benefits.", "content": "Decay of radioactive isotopes by K-capture leads to the Auger effect and results in the loss of several orbital electrons and the emission of X-rays. Whereas radiation effects are produced from the emitted electrons, the consequences of the Auger effect are strictly localized to the site of the decaying nuclide. The paper reviews the biological consequences of the decay of 125I which produces the Auger effect. Nearly all data were obtained from DNA labeled with 125I-5-iodo-2'-deoxyuridine (IUdR) in bacteria and mammalian cells. Parameters of effects were cell death, DNA strand breaks, and mutation induction. In order to recognize in a cell the contribution from the Auger effect and that of absorbed radiation, experimental data are analysed in terms of the specific energy for the nuclear volume which contains the isotope. The data indicate that decay of 125I is far more toxic than is expected on the basis of absorbed dose to the labeled nucleus. Moreover, it is emphasized that the toxicity of the 125I decay is largely determined by events immediately localized to the site of decay. Because the consequences of the Auger effect are stricly localized to the molecular site of the decay, 125I and perhaps other nuclides decaying by K-capture promise to be interesting tools in cell biology and molecular biology. First data on the Auger effect as a tool are summarized. It appears that recognizable biological damage is only observed when the Auger effect takes place in vitally important molecules, an example of which is DNA.", "contents": "Biological damage from the Auger effect, possible benefits. Decay of radioactive isotopes by K-capture leads to the Auger effect and results in the loss of several orbital electrons and the emission of X-rays. Whereas radiation effects are produced from the emitted electrons, the consequences of the Auger effect are strictly localized to the site of the decaying nuclide. The paper reviews the biological consequences of the decay of 125I which produces the Auger effect. Nearly all data were obtained from DNA labeled with 125I-5-iodo-2'-deoxyuridine (IUdR) in bacteria and mammalian cells. Parameters of effects were cell death, DNA strand breaks, and mutation induction. In order to recognize in a cell the contribution from the Auger effect and that of absorbed radiation, experimental data are analysed in terms of the specific energy for the nuclear volume which contains the isotope. The data indicate that decay of 125I is far more toxic than is expected on the basis of absorbed dose to the labeled nucleus. Moreover, it is emphasized that the toxicity of the 125I decay is largely determined by events immediately localized to the site of decay. Because the consequences of the Auger effect are stricly localized to the molecular site of the decay, 125I and perhaps other nuclides decaying by K-capture promise to be interesting tools in cell biology and molecular biology. First data on the Auger effect as a tool are summarized. It appears that recognizable biological damage is only observed when the Auger effect takes place in vitally important molecules, an example of which is DNA."}
{"id": "PMID:1101291", "title": "[Pathophysiology of renal hypertension (author's transl)].", "content": "The review discusses the pathophysiology of the renal mechanisms of blood-pressure control. The physiology of the renin-angiotensin system is described and the mineralo-corticoid function has also been considered. The implications of alterations of these systems in various nephropathies are briefly summarized (renal artery stenosis, infarction, Page's syndrome, acute glomerulonephritis, essential hypertension a.o.).", "contents": "[Pathophysiology of renal hypertension (author's transl)]. The review discusses the pathophysiology of the renal mechanisms of blood-pressure control. The physiology of the renin-angiotensin system is described and the mineralo-corticoid function has also been considered. The implications of alterations of these systems in various nephropathies are briefly summarized (renal artery stenosis, infarction, Page's syndrome, acute glomerulonephritis, essential hypertension a.o.)."}
{"id": "PMID:1101293", "title": "[Pancreas scan (author's transl)].", "content": "A Review and a report of our ten years experience with pancreas scans. The diagnostic value of 75Selen-Methionine (75Se) scan is limited because of the lack of organ specificity of this substance. There is no improvement by pharmacological stimulation of the pancreas or subtraction techniques of the 75Se-activity in the liver. In the early phase after the injection of 75Se the distribution is more favourable for the pancreas compared to liver and intestine. Four descriptive terms are in use: normal, spotted, organ defect, and lack of uptake. There are no specific signs for pancreatitis, carcinoma, or exocrine dysfunction. Considering these limiting factors the use of pancreas scans is valuable to exclude pancreatic diseases.", "contents": "[Pancreas scan (author's transl)]. A Review and a report of our ten years experience with pancreas scans. The diagnostic value of 75Selen-Methionine (75Se) scan is limited because of the lack of organ specificity of this substance. There is no improvement by pharmacological stimulation of the pancreas or subtraction techniques of the 75Se-activity in the liver. In the early phase after the injection of 75Se the distribution is more favourable for the pancreas compared to liver and intestine. Four descriptive terms are in use: normal, spotted, organ defect, and lack of uptake. There are no specific signs for pancreatitis, carcinoma, or exocrine dysfunction. Considering these limiting factors the use of pancreas scans is valuable to exclude pancreatic diseases."}
{"id": "PMID:1101294", "title": "Rapid computer-aided radiographic calculation of total lung capacity (TLC).", "content": "Methods currently used for determining total lung capacity are either simple but inaccurate in the presence of airways disease (gas dilution) or accurate but not generally available (body plethysmography). The manual radiographic method is accurate both in normals and in patients with airways disease but is very tedious to use. The authors have developed a semi-automatic radiographic method utilizing a position transducer and a small computer which reduces the time for each determination from approximately 20 min. to 1 min. Agreement with manual calculations in 80 controls and 80 patients with airways disease is excellent (average correlation coefficient, 0.9872; average residual error, 2.65% or 156 ml).", "contents": "Rapid computer-aided radiographic calculation of total lung capacity (TLC). Methods currently used for determining total lung capacity are either simple but inaccurate in the presence of airways disease (gas dilution) or accurate but not generally available (body plethysmography). The manual radiographic method is accurate both in normals and in patients with airways disease but is very tedious to use. The authors have developed a semi-automatic radiographic method utilizing a position transducer and a small computer which reduces the time for each determination from approximately 20 min. to 1 min. Agreement with manual calculations in 80 controls and 80 patients with airways disease is excellent (average correlation coefficient, 0.9872; average residual error, 2.65% or 156 ml)."}
{"id": "PMID:1101329", "title": "Effects of delta-9-tetrahydrocannabinol on mouse spleens.", "content": "A study was undertaken to ascertain the effects of delta-9-Tetrahydrocannabinol on the plaque forming cell response of mice immunized with sheep erythrocytes. It was found that a marked suppression of plaque forming cells occurred concomitant with a general loss of spleen cellularity following administration of delta-9-THC. These results suggest rather marked effects of this drug on antibody synthesis.", "contents": "Effects of delta-9-tetrahydrocannabinol on mouse spleens. A study was undertaken to ascertain the effects of delta-9-Tetrahydrocannabinol on the plaque forming cell response of mice immunized with sheep erythrocytes. It was found that a marked suppression of plaque forming cells occurred concomitant with a general loss of spleen cellularity following administration of delta-9-THC. These results suggest rather marked effects of this drug on antibody synthesis."}
{"id": "PMID:1101330", "title": "Dynamic alveolar mechanics as studied by videomicroscopy.", "content": "The in vivo morphology of rat alveoli has been recorded and studied on videotape. The dynamic internal structure has been compared to that observed by histologic section from lung rapidly frozen with liquid nitrogen. The irreversible nature of local alveolar wall movement has been demonstrated in animals ventilated both with and without positive end-expiratory pressure (10 cm water) by parametrically comparing the distances between two sets of paired points measured sequentially across internal alveolar surfaces at intervals of 0.017 sec. Geometric hysteresis was significantly less in the animals ventilated with positive end-expiratory pressure. This hysteresis can be explained by irreversibility in alveolar surface area apart from any irreversible dependence on surface tension. Sharp reverses and rapid changes in geometric hysteresis suggest that the length-tension properties of elastic tissue within the alveolar wall and interface forces from adjacent alveoli are important determinants of local alveolar wall motion and pulmonary hysteresis.", "contents": "Dynamic alveolar mechanics as studied by videomicroscopy. The in vivo morphology of rat alveoli has been recorded and studied on videotape. The dynamic internal structure has been compared to that observed by histologic section from lung rapidly frozen with liquid nitrogen. The irreversible nature of local alveolar wall movement has been demonstrated in animals ventilated both with and without positive end-expiratory pressure (10 cm water) by parametrically comparing the distances between two sets of paired points measured sequentially across internal alveolar surfaces at intervals of 0.017 sec. Geometric hysteresis was significantly less in the animals ventilated with positive end-expiratory pressure. This hysteresis can be explained by irreversibility in alveolar surface area apart from any irreversible dependence on surface tension. Sharp reverses and rapid changes in geometric hysteresis suggest that the length-tension properties of elastic tissue within the alveolar wall and interface forces from adjacent alveoli are important determinants of local alveolar wall motion and pulmonary hysteresis."}
{"id": "PMID:1101346", "title": "[Polygraphic recording of tremor and of hypertonic phenomena. Application to the action of various drugs].", "content": "The recording technique described makes possible the detailed analysis of hypertonia and tremor in Parkinson's disease, as well as the action of different drugs. It may contribute to the understanding of the physiopathological basis of these motor disturbances and the mode of action of anti-parkinsonian drugs. It also represents a reliable method of repeated observation, independent of subjective factors, in the evaluation of the long-term effectiveness of a medication. We were particularly concerned with the evaluation and comparison of the effect of different drugs in the course of a short study, involving the administration of a single dose of the medications used. In this way were studied: Apomorphine, given sub-cutaneously in non-emetic doses, which decreases in a constant and spectacular fashion both tremor and hypertonia ; Piribedil (1-3 mg IV) ; L-Dopa (100 mg IV) ; Ro-080576/007 F in a dose of one capsule containing 200 mg of L-Dopa and 50 mg of L-dopa-decarboxylase inhibitor. The effect of these various medications was invariable greater than that of a placebo and lasted much longer. With these doses, and under the special experimental conditions of this study, Piribedil was better tolerated and in general more active than L-dopa, in particular in relation to tremor. The action observed in the course of this short study prior to treatment might, to a certain extent, make it possible to predict the effect of long-term treatment.", "contents": "[Polygraphic recording of tremor and of hypertonic phenomena. Application to the action of various drugs]. The recording technique described makes possible the detailed analysis of hypertonia and tremor in Parkinson's disease, as well as the action of different drugs. It may contribute to the understanding of the physiopathological basis of these motor disturbances and the mode of action of anti-parkinsonian drugs. It also represents a reliable method of repeated observation, independent of subjective factors, in the evaluation of the long-term effectiveness of a medication. We were particularly concerned with the evaluation and comparison of the effect of different drugs in the course of a short study, involving the administration of a single dose of the medications used. In this way were studied: Apomorphine, given sub-cutaneously in non-emetic doses, which decreases in a constant and spectacular fashion both tremor and hypertonia ; Piribedil (1-3 mg IV) ; L-Dopa (100 mg IV) ; Ro-080576/007 F in a dose of one capsule containing 200 mg of L-Dopa and 50 mg of L-dopa-decarboxylase inhibitor. The effect of these various medications was invariable greater than that of a placebo and lasted much longer. With these doses, and under the special experimental conditions of this study, Piribedil was better tolerated and in general more active than L-dopa, in particular in relation to tremor. The action observed in the course of this short study prior to treatment might, to a certain extent, make it possible to predict the effect of long-term treatment."}
{"id": "PMID:1101351", "title": "[Aseptic osteonecrosis in renal transplantation ; apropos of 29 cases].", "content": "Over a period of observation of 14 years, one or more aspetic osteonecroses (AON) were discovered in 29 patients who had undergone renal transplantation. Altogether 64 AON were discovered. In patients who had survived at least 1 year, the frequency of this lesion was 18 percent. These transplantation necroses were often bilateral (16 cases out of 21 AON of the femoral head) or multiple (6 patients presenting AON in more than 3 sites) ; necroses were sometimes found in unusual sites (shoulder, ankle, upper end of the tibia, scaphoid, condyle of the humerus). The AONs of the lower limbs were remarkable because of their extent and because of the rapid evolution of the radiological signs. This is in contrast to the moderate nature of the pain. Latent types of necrosis are also found (3 cases of AON of the femoral head). During a search for factors favouring the development of AON, the authors noted that the average duration of haemodialysis was longer in the patients with AON than in a control group of transplant patients. The average daily dose of corticoids for the first six months after transplantation was not significantly different in the two groups studied. However, the 15 patients with transplants who were not given corticoids did not develop AON.", "contents": "[Aseptic osteonecrosis in renal transplantation ; apropos of 29 cases]. Over a period of observation of 14 years, one or more aspetic osteonecroses (AON) were discovered in 29 patients who had undergone renal transplantation. Altogether 64 AON were discovered. In patients who had survived at least 1 year, the frequency of this lesion was 18 percent. These transplantation necroses were often bilateral (16 cases out of 21 AON of the femoral head) or multiple (6 patients presenting AON in more than 3 sites) ; necroses were sometimes found in unusual sites (shoulder, ankle, upper end of the tibia, scaphoid, condyle of the humerus). The AONs of the lower limbs were remarkable because of their extent and because of the rapid evolution of the radiological signs. This is in contrast to the moderate nature of the pain. Latent types of necrosis are also found (3 cases of AON of the femoral head). During a search for factors favouring the development of AON, the authors noted that the average duration of haemodialysis was longer in the patients with AON than in a control group of transplant patients. The average daily dose of corticoids for the first six months after transplantation was not significantly different in the two groups studied. However, the 15 patients with transplants who were not given corticoids did not develop AON."}
{"id": "PMID:1101352", "title": "[Systematic study of sequential salivary gland scintigraphy during rheumatoid arthritis and collagenosis. Apropos of 150 examinations].", "content": "Sequential salivary scintigraphy has been practised systematically in 130 patients hospitalized in a rheumatology unit. These patients were suffering from typical, classical rheumatoid arthritis, rheumatoid arthritis with high positivity for anti-nuclear antibodies, clearly defined polyarthritis, systemic scleroderma, other types of collagenosis, forms of inflammatory polyarticular rheumatism other than rheumatoid arthritis and collagenosis, and from different degenerative diseases. The scintigraphic anomalies were divided in three stages according to a personal classification. Nine patients presented a patend Gougerot-Sj\u00f6gren syndrome. All of them had abnormal or very abnormal scintigraphy. However, the patients with classical rheumatoid arthritis without signs of the Gougerot-Sj\u00f6gren syndrome, also showed a high frequency of isotopic anomalies that were equally severe. These were clearly connected only with the particular diagnosis (with classical rheumatoid arthritis, but not with clearly defined polyarthritis or collagenosis). No other direct relationship could be established between these diseases and the clinical, radiological, laboratory, or therapeutic aspects of the case. On the other hand, they were often associated with a reduction in lacrymal and salivary secretion. These isotopic studies, in combination with other findings shed a new light on the Gougerot-Sj\u00f6gren syndrome, taking into account the diffusion of the exocrine lesions and of the contributions of the most modern methods of investigation.", "contents": "[Systematic study of sequential salivary gland scintigraphy during rheumatoid arthritis and collagenosis. Apropos of 150 examinations]. Sequential salivary scintigraphy has been practised systematically in 130 patients hospitalized in a rheumatology unit. These patients were suffering from typical, classical rheumatoid arthritis, rheumatoid arthritis with high positivity for anti-nuclear antibodies, clearly defined polyarthritis, systemic scleroderma, other types of collagenosis, forms of inflammatory polyarticular rheumatism other than rheumatoid arthritis and collagenosis, and from different degenerative diseases. The scintigraphic anomalies were divided in three stages according to a personal classification. Nine patients presented a patend Gougerot-Sj\u00f6gren syndrome. All of them had abnormal or very abnormal scintigraphy. However, the patients with classical rheumatoid arthritis without signs of the Gougerot-Sj\u00f6gren syndrome, also showed a high frequency of isotopic anomalies that were equally severe. These were clearly connected only with the particular diagnosis (with classical rheumatoid arthritis, but not with clearly defined polyarthritis or collagenosis). No other direct relationship could be established between these diseases and the clinical, radiological, laboratory, or therapeutic aspects of the case. On the other hand, they were often associated with a reduction in lacrymal and salivary secretion. These isotopic studies, in combination with other findings shed a new light on the Gougerot-Sj\u00f6gren syndrome, taking into account the diffusion of the exocrine lesions and of the contributions of the most modern methods of investigation."}
{"id": "PMID:1101355", "title": "[Treatment of a case of extreme micrognathia. Significance of continuous traction].", "content": "A case of very marked mandibulary atrophy was corrected by osteotomy and bone grafting of the ascending ramus. This procedure was performed in two stages with continuous traction of the mandible. This traction avoided the retro-displacement caused by the skin and muscle contracture and stabilized the post-operative immobilization.", "contents": "[Treatment of a case of extreme micrognathia. Significance of continuous traction]. A case of very marked mandibulary atrophy was corrected by osteotomy and bone grafting of the ascending ramus. This procedure was performed in two stages with continuous traction of the mandible. This traction avoided the retro-displacement caused by the skin and muscle contracture and stabilized the post-operative immobilization."}
{"id": "PMID:1101362", "title": "Comparison of five growth media and two anaerobic techniques for isolating bacteria from dental plaque.", "content": "Five agar media in combination with the Hungate roll tube technique and conventional anaerobic jar technique (Baird & Tatlock\u00bf jars) were evaluated to determine the most suitable medium for non-selective isolation of the viable microorganisms in dental plaque. The highest colony count was obtained by using \"Modified Medium 10\" with the roll tube technique. About twice as many plaque colonies were recovered by roll tube technique as by the conventional anaerobic technique. With the MM 10 roll tube technique, 7 d of incubation revealed only 93% of the colonies that could be detected after 14 d of incubation.", "contents": "Comparison of five growth media and two anaerobic techniques for isolating bacteria from dental plaque. Five agar media in combination with the Hungate roll tube technique and conventional anaerobic jar technique (Baird & Tatlock\u00bf jars) were evaluated to determine the most suitable medium for non-selective isolation of the viable microorganisms in dental plaque. The highest colony count was obtained by using \"Modified Medium 10\" with the roll tube technique. About twice as many plaque colonies were recovered by roll tube technique as by the conventional anaerobic technique. With the MM 10 roll tube technique, 7 d of incubation revealed only 93% of the colonies that could be detected after 14 d of incubation."}
{"id": "PMID:1101363", "title": "Effect of 2-years' use of chlorhexidine-containing dentifrices on plaque, gingivitis, and caries.", "content": "Chlorhexidine digluconate in 0.1% and 0.4% concentrations was added to dentifrices to evaluate its effect on plaque formation, gingival conditions and caries in 73 dental students over a 2-year period. No differences were found in the Pl I and the G I indices between the active and the placebo dentifrices, but a possible effect on caries was demonstrated. Discolorations of anterior teeth and fillings were the only side effects observed.", "contents": "Effect of 2-years' use of chlorhexidine-containing dentifrices on plaque, gingivitis, and caries. Chlorhexidine digluconate in 0.1% and 0.4% concentrations was added to dentifrices to evaluate its effect on plaque formation, gingival conditions and caries in 73 dental students over a 2-year period. No differences were found in the Pl I and the G I indices between the active and the placebo dentifrices, but a possible effect on caries was demonstrated. Discolorations of anterior teeth and fillings were the only side effects observed."}
{"id": "PMID:1101357", "title": "[Experimental study on the role of the mylohyoid muscle in the instability of dental prostheses].", "content": "Using cinematographic and radiocinematographic recording techniques, the authors studied the movements of inferior prosthesis unstable in the lingual region. Pronunciation of the diphthong La... La... La... causes upward and forward projection of the prosthesis. Electromyography records contraction of the mylo-hyoid during pronunciation of these diphthongs. In the totally edentulous patient, the crest of the mylo-hoid is very close to the alveolar crest. From a clinical standpoint, functional exploration of the mylohyoid is easy, the index finger being placed along the mylo-hyoid crest and the patient asked to say La... La... La... If the index finger is pushed forwards and up, surgical intervention to lower the mylo-hyoid (Trauner's operation) is indicated, in association or not with other procedures designed to treat the other causes of instability or the prosthesis.", "contents": "[Experimental study on the role of the mylohyoid muscle in the instability of dental prostheses]. Using cinematographic and radiocinematographic recording techniques, the authors studied the movements of inferior prosthesis unstable in the lingual region. Pronunciation of the diphthong La... La... La... causes upward and forward projection of the prosthesis. Electromyography records contraction of the mylo-hyoid during pronunciation of these diphthongs. In the totally edentulous patient, the crest of the mylo-hoid is very close to the alveolar crest. From a clinical standpoint, functional exploration of the mylohyoid is easy, the index finger being placed along the mylo-hyoid crest and the patient asked to say La... La... La... If the index finger is pushed forwards and up, surgical intervention to lower the mylo-hyoid (Trauner's operation) is indicated, in association or not with other procedures designed to treat the other causes of instability or the prosthesis."}
{"id": "PMID:1101359", "title": "[Retention of a complete denture by the enclave method. Convergent enclaves].", "content": "The authors propose a technical variant of the method of enclaves for the retention of the full denture, this in the inferior maxilla because it is there that the problem of retention is posed with the greatest acuity and frequency. But whereas in the processes described the enclaves, 2 in number, corresponding with the zone of implantation of the canines, are perpendicular to the mandible in the frontal and sagittal planes, in the technique explained here each of the enclaves makes with the mandible, in the frontal plane, an angle of 10 degrees with respect to the median axis, thus leading to a convergence and hence an increased retention of the prosthesis with which they are connected.", "contents": "[Retention of a complete denture by the enclave method. Convergent enclaves]. The authors propose a technical variant of the method of enclaves for the retention of the full denture, this in the inferior maxilla because it is there that the problem of retention is posed with the greatest acuity and frequency. But whereas in the processes described the enclaves, 2 in number, corresponding with the zone of implantation of the canines, are perpendicular to the mandible in the frontal and sagittal planes, in the technique explained here each of the enclaves makes with the mandible, in the frontal plane, an angle of 10 degrees with respect to the median axis, thus leading to a convergence and hence an increased retention of the prosthesis with which they are connected."}
{"id": "PMID:1101365", "title": "Gastric secretory response to graded doses of pentagastrin alone or in combination with carbacholine in unoperated duodenal ulcer patients.", "content": "Dose-response studies of pentagastrin-stimulated secretion were performed in 6 unoperated duodenal ulcer patients. On different days pentagastrin was given in doses of 0.15, 1.5, and 15 mug/kg/hr, either alone or in combination with carbacholine, 2 mug/kg/hr. There were no significant differences between mean acid or pepsin outputs or mean volumes of gastric juice after infusion of pentagastrin alone or pentagastrin plus carbacholine at any dose step of pentagastrin. The dose of pentagastrin required to elicit half maximal acid output (Km) and the calculated maximal acid output (Vmax) were the same for pentagastrin alone and pentgastrin plus carbacholine. Thus, carbacholine did not increase the sensitivity of the parietal cells to pentagastrin in the unoperated duodenal ulcer patients.", "contents": "Gastric secretory response to graded doses of pentagastrin alone or in combination with carbacholine in unoperated duodenal ulcer patients. Dose-response studies of pentagastrin-stimulated secretion were performed in 6 unoperated duodenal ulcer patients. On different days pentagastrin was given in doses of 0.15, 1.5, and 15 mug/kg/hr, either alone or in combination with carbacholine, 2 mug/kg/hr. There were no significant differences between mean acid or pepsin outputs or mean volumes of gastric juice after infusion of pentagastrin alone or pentagastrin plus carbacholine at any dose step of pentagastrin. The dose of pentagastrin required to elicit half maximal acid output (Km) and the calculated maximal acid output (Vmax) were the same for pentagastrin alone and pentgastrin plus carbacholine. Thus, carbacholine did not increase the sensitivity of the parietal cells to pentagastrin in the unoperated duodenal ulcer patients."}
{"id": "PMID:1101366", "title": "An inhibitor of chemotaxis and phagocytosis in reticulum cell sarcoma.", "content": "In a study of neutrophil functions in haematological disorders using in vitro techniques, a heat-stable inhibitor of chemotaxis and phagocytosis was demonstrated in the plasma and serum of a 64-year-old woman with reticulum cell sarcoma. In partial purification by chromatography, the inhibitor activity could not be separated from IgG. Clinically the patient did not exhibit abnormal susceptibility to infections.", "contents": "An inhibitor of chemotaxis and phagocytosis in reticulum cell sarcoma. In a study of neutrophil functions in haematological disorders using in vitro techniques, a heat-stable inhibitor of chemotaxis and phagocytosis was demonstrated in the plasma and serum of a 64-year-old woman with reticulum cell sarcoma. In partial purification by chromatography, the inhibitor activity could not be separated from IgG. Clinically the patient did not exhibit abnormal susceptibility to infections."}
{"id": "PMID:1101367", "title": "In vitro inhibition of endotoxin induced platelet aggregation with hydrocortisone sodium succinate (Solu-Cortef).", "content": "Citrated canine platelet rich plasma stimulated with ADP, thrombin, collagen and E. coli endotoxin demonstrated platelet aggregation when measured with Payton aggregometer. Following incubation with hydrocortisone sodium succinate (Solu-Cortef) the same platelet rich plasma did not undergo platelet aggregation when stimulated by these agents. Solu-Cortef added to platelet rich plasma during ATP, thrombin, collagen and endotoxin stimulated platelet aggregation resulted in a reversal of the optical density suggesting deaggregation. These in vitro observations support the beneficial effects observed when steroids are administered in vivo to experimental or clinical shock.", "contents": "In vitro inhibition of endotoxin induced platelet aggregation with hydrocortisone sodium succinate (Solu-Cortef). Citrated canine platelet rich plasma stimulated with ADP, thrombin, collagen and E. coli endotoxin demonstrated platelet aggregation when measured with Payton aggregometer. Following incubation with hydrocortisone sodium succinate (Solu-Cortef) the same platelet rich plasma did not undergo platelet aggregation when stimulated by these agents. Solu-Cortef added to platelet rich plasma during ATP, thrombin, collagen and endotoxin stimulated platelet aggregation resulted in a reversal of the optical density suggesting deaggregation. These in vitro observations support the beneficial effects observed when steroids are administered in vivo to experimental or clinical shock."}
{"id": "PMID:1101368", "title": "Complement activation in relation to protective function of antibodies to Escherichia coli O antigens.", "content": "The relation between complement activation in an indirect hemolysis assay of E. coli O antibodies and their protective function in experimentally infected mice was analyzed. The antibody amounts and avidities were estimated in antisera and in IgG and IgM antibody fractions by the ammonium sulphate precipitation (ASP) technique of Farr. The protection obtained was due to both IgG and IgM antibodies and showed a close relation to the quantity of antibodies measured by the ASP technique. No link between complement activation capacity of the antibodies and their protective function could be seen. In the system used, the IgM antibodies showed a correlation between antibody avidity and complement activating efficiency, whereas the IgG antibodies activated complement poorly. The results do not indicate a major function of the complement in antibody-mediated protection against E. coli in this experimental system in mice.", "contents": "Complement activation in relation to protective function of antibodies to Escherichia coli O antigens. The relation between complement activation in an indirect hemolysis assay of E. coli O antibodies and their protective function in experimentally infected mice was analyzed. The antibody amounts and avidities were estimated in antisera and in IgG and IgM antibody fractions by the ammonium sulphate precipitation (ASP) technique of Farr. The protection obtained was due to both IgG and IgM antibodies and showed a close relation to the quantity of antibodies measured by the ASP technique. No link between complement activation capacity of the antibodies and their protective function could be seen. In the system used, the IgM antibodies showed a correlation between antibody avidity and complement activating efficiency, whereas the IgG antibodies activated complement poorly. The results do not indicate a major function of the complement in antibody-mediated protection against E. coli in this experimental system in mice."}
{"id": "PMID:1101369", "title": "Effect of polyclonal B-cell activators on DNA synthesis in fibroblasts.", "content": "The polyclonal B-cell activators (PBA) lipopolysaccharide (LPS), type III pneumococcal polysaccharide (SIII), dextran, dextran sulphate, pentosan sulphate, and polyvinylpyrrolidone (PVP) were found to cause an increased DNA synthesis in mouse fibroblast monolayer cultures in the presence of calf serum, provided the background cpm were within certain values. The same PBA suppressed DNA synthesis when the cultures showed high background [corrected] cpm. Since the PBA did not activate DNA synthesis in serum-free cultures, it was concluded that PBA could be directly activate fibroblasts but rather influenced factors responsible for regulation of DNA synthesis in fibroblasts. In contrast PBA directly trigger lymphocytes.", "contents": "Effect of polyclonal B-cell activators on DNA synthesis in fibroblasts. The polyclonal B-cell activators (PBA) lipopolysaccharide (LPS), type III pneumococcal polysaccharide (SIII), dextran, dextran sulphate, pentosan sulphate, and polyvinylpyrrolidone (PVP) were found to cause an increased DNA synthesis in mouse fibroblast monolayer cultures in the presence of calf serum, provided the background cpm were within certain values. The same PBA suppressed DNA synthesis when the cultures showed high background [corrected] cpm. Since the PBA did not activate DNA synthesis in serum-free cultures, it was concluded that PBA could be directly activate fibroblasts but rather influenced factors responsible for regulation of DNA synthesis in fibroblasts. In contrast PBA directly trigger lymphocytes."}
{"id": "PMID:1101370", "title": "Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity.", "content": "The production of antibody by human colostral cells was assayed by the hemolysis in-gel technique. When sheep erythrocytes coated with O antigens from frequently encountered Escherichia coli bacteria were used as detector cells and anti-IgA serum was added for development, numerous plaque-forming cells (PFC) were demonstrated in all samples tested. In contrast, plaques were rarely seen in the presence of anti-IgG developing serum. The direct (IgM) plaques occasionally noted with both antigen-coated and uncoated sheep erythrocytes were mainly due to the production of heterophil antibodies, since they were not formed when human erythrocytes were used as O-antigen carriers. A strikingly high number of the colostral lymphocytes formed antibodies to the E. coli antigens, up to 8%. This suggests that these cells represent a rather selective population--possibly cells from the gastrointestinal tract exposed to enteric bacteria. The large number of plaques observed, the predominance of the cells forming IgA antibodies, and the marked changes in PFC number in relationship to parturition pose a number of questions relevant to the antibody-producing colostrum cells and their relationship to the secretory immune system.", "contents": "Antibody production by human colostral cells. I. Immunoglobulin class, specificity, and quantity. The production of antibody by human colostral cells was assayed by the hemolysis in-gel technique. When sheep erythrocytes coated with O antigens from frequently encountered Escherichia coli bacteria were used as detector cells and anti-IgA serum was added for development, numerous plaque-forming cells (PFC) were demonstrated in all samples tested. In contrast, plaques were rarely seen in the presence of anti-IgG developing serum. The direct (IgM) plaques occasionally noted with both antigen-coated and uncoated sheep erythrocytes were mainly due to the production of heterophil antibodies, since they were not formed when human erythrocytes were used as O-antigen carriers. A strikingly high number of the colostral lymphocytes formed antibodies to the E. coli antigens, up to 8%. This suggests that these cells represent a rather selective population--possibly cells from the gastrointestinal tract exposed to enteric bacteria. The large number of plaques observed, the predominance of the cells forming IgA antibodies, and the marked changes in PFC number in relationship to parturition pose a number of questions relevant to the antibody-producing colostrum cells and their relationship to the secretory immune system."}
{"id": "PMID:1101371", "title": "Acute epiglottitis; a clinical, bacteriological and serological study.", "content": "25 patients, 15 children and 10 adults, with the clinical diagnosis of acute epiglottitis were studied. Patients severely ill on admission, 13 children and 1 adult, were immediately treated with tracheotomy and antibiotics; the remaining patients with antibiotics only. Haemophilus influenzae type b was the causative organism in all children and in 3 adults. One adult had a Diplococcus pneumoniae infection. No pathogens were isolated from the 6 remaining patients. Four of the children developed H. influenzae type b meningitis. All patients recovered. Anti-b antibodies were not demonstrable in any initial serum sample while agglutinating anti-b antibodies were found in subsequent samples (indirect hemagglutination). All patients with a type b infection had already initially precipitating anti-O antibodies against their own strain, and in most cases complement-fixing anti-O antibodies (mixed H. influenzae O antigen) with rising titers in subsequent serum samples. A possible connection between the presence of anti-O antibodies initially and the development of the acute epiglottitis is discussed.", "contents": "Acute epiglottitis; a clinical, bacteriological and serological study. 25 patients, 15 children and 10 adults, with the clinical diagnosis of acute epiglottitis were studied. Patients severely ill on admission, 13 children and 1 adult, were immediately treated with tracheotomy and antibiotics; the remaining patients with antibiotics only. Haemophilus influenzae type b was the causative organism in all children and in 3 adults. One adult had a Diplococcus pneumoniae infection. No pathogens were isolated from the 6 remaining patients. Four of the children developed H. influenzae type b meningitis. All patients recovered. Anti-b antibodies were not demonstrable in any initial serum sample while agglutinating anti-b antibodies were found in subsequent samples (indirect hemagglutination). All patients with a type b infection had already initially precipitating anti-O antibodies against their own strain, and in most cases complement-fixing anti-O antibodies (mixed H. influenzae O antigen) with rising titers in subsequent serum samples. A possible connection between the presence of anti-O antibodies initially and the development of the acute epiglottitis is discussed."}
{"id": "PMID:1101372", "title": "Bacteremia in a general hospital. A prospective study of 102 consecutive cases.", "content": "A prospective clinical-bacteriological study of 102 consecutive cases of confirmed bacteremia at a Copenhagen City general hospital was carried out during 5 months of 1973 with special concern given to focus of infection and acquisition of microorganisms. Valid positive cultures were obtained from 7.2 patients per 1000 admissions. 50 of the 102 bacteremias were by all probability acquired in the hospital, mainly due to transurethral manipulations or intravenous lines. Pneumonia and hepatobiliary infections accounted for most of the non-hospital acquired bacteremias. 26/102 patients died in relation to the bacteremia. Escherichia coli and Staphylococcus aureus caused more than half of the infections. Bacteremia caused by proteus, klebsiella, enterobacter species of staphylococci was in most cases nosocomial and carried the highest mortality, i.e. 40%, verus 15% when other organisms were responsible. It is concluded that nosocomial bacteremia is a frequent and life-endangering complication which is often preceded by certain diagnostic or therapeutic procedures, not invariably linked to severe underlying diseases. Consequently, attempts to reduce bacteremic episodes should include surveillance of ecological factors and certain hospital procedures.", "contents": "Bacteremia in a general hospital. A prospective study of 102 consecutive cases. A prospective clinical-bacteriological study of 102 consecutive cases of confirmed bacteremia at a Copenhagen City general hospital was carried out during 5 months of 1973 with special concern given to focus of infection and acquisition of microorganisms. Valid positive cultures were obtained from 7.2 patients per 1000 admissions. 50 of the 102 bacteremias were by all probability acquired in the hospital, mainly due to transurethral manipulations or intravenous lines. Pneumonia and hepatobiliary infections accounted for most of the non-hospital acquired bacteremias. 26/102 patients died in relation to the bacteremia. Escherichia coli and Staphylococcus aureus caused more than half of the infections. Bacteremia caused by proteus, klebsiella, enterobacter species of staphylococci was in most cases nosocomial and carried the highest mortality, i.e. 40%, verus 15% when other organisms were responsible. It is concluded that nosocomial bacteremia is a frequent and life-endangering complication which is often preceded by certain diagnostic or therapeutic procedures, not invariably linked to severe underlying diseases. Consequently, attempts to reduce bacteremic episodes should include surveillance of ecological factors and certain hospital procedures."}
{"id": "PMID:1101375", "title": "Comparative double-blind trial of intra-articular injections of two long-acting forms of betamethasone.", "content": "Two injectable betamethasone preparations, betamethasone dipropionate/betamethasone phosphate and betamethasone acetate/betamethasone phosphate were compared in a controlled trial on 22 \"matched patient pairs\" with rheumatic diseases. The two preparations were injected intra-articularly in knee joints affected by active synovitis. The time until onset of action and the duration of clinically adequate anti-inflammatory effect were recorded. All injections produced clinically significant effects. The interval before recurrence of symptoms varied from 2 days to more than 3 months. The dipropionate form showed statistically significantly longer duration of action. No difference was noted in the time until onset of action. Local side effects were not observed. The double-blind method of clinical evaluation proved useful in comparing duration of action between long-acting corticosteroids.", "contents": "Comparative double-blind trial of intra-articular injections of two long-acting forms of betamethasone. Two injectable betamethasone preparations, betamethasone dipropionate/betamethasone phosphate and betamethasone acetate/betamethasone phosphate were compared in a controlled trial on 22 \"matched patient pairs\" with rheumatic diseases. The two preparations were injected intra-articularly in knee joints affected by active synovitis. The time until onset of action and the duration of clinically adequate anti-inflammatory effect were recorded. All injections produced clinically significant effects. The interval before recurrence of symptoms varied from 2 days to more than 3 months. The dipropionate form showed statistically significantly longer duration of action. No difference was noted in the time until onset of action. Local side effects were not observed. The double-blind method of clinical evaluation proved useful in comparing duration of action between long-acting corticosteroids."}
{"id": "PMID:1101376", "title": "Use of pectoralis skin pedicle flap for closure of large bronchial fistula connected with postpneumonectomy empyema.", "content": "In 4 patients, the postpneumonectomy empyema was connected with a large bronchopleural fistula. The empyema was in all cases treated by a permanent open window thoracostoma. The fistulae were closed later with pedicle flaps made of the pectoralis muscle and the adjoining skin. In 2 patients the closing of the bronchial fistula was successful, and the treatment of one patient is not completed. In one patient the open pneural cavity was covered completely by skin using an additional pedicle flap and free skin transplantation. The surgical technique of the pedicle flap operation is described and the cases are reported.", "contents": "Use of pectoralis skin pedicle flap for closure of large bronchial fistula connected with postpneumonectomy empyema. In 4 patients, the postpneumonectomy empyema was connected with a large bronchopleural fistula. The empyema was in all cases treated by a permanent open window thoracostoma. The fistulae were closed later with pedicle flaps made of the pectoralis muscle and the adjoining skin. In 2 patients the closing of the bronchial fistula was successful, and the treatment of one patient is not completed. In one patient the open pneural cavity was covered completely by skin using an additional pedicle flap and free skin transplantation. The surgical technique of the pedicle flap operation is described and the cases are reported."}
{"id": "PMID:1101377", "title": "Toxicity and pollution potential of thallium.", "content": "Toxicity and pollution potential of thallium are reviewed. Thallium is slightly more acutely toxic to mammals than mercury, and as acutely toxic as copper to fish. Its present industrial uses are too limited to generate pollution, but thallium, discharged in wastes from mines, ore-processing, and coal-burning plants, is contaminating the environment.", "contents": "Toxicity and pollution potential of thallium. Toxicity and pollution potential of thallium are reviewed. Thallium is slightly more acutely toxic to mammals than mercury, and as acutely toxic as copper to fish. Its present industrial uses are too limited to generate pollution, but thallium, discharged in wastes from mines, ore-processing, and coal-burning plants, is contaminating the environment."}
{"id": "PMID:1101378", "title": "Lymphocyte surface immunoglobulins.", "content": "Immunoglobulins have been isolated from the surface of B (bone marrow-derived) and T (thymus-derived) lymphocytes. Two types of membrane immunoglobulin occur on B lymphocytes; one type resembles the 200,000-dalton subunit of IgM, the second possesses a heavy chain electrophoretically distinct from mu chain and does not correspond to any of the known classes of mouse immunoglobulins. It might correspond to human sigma chain. T lymphocytes possess only one type of surface immunoglobulin. This molecule has a mass of approximately 200,000 daltons and contains light chains and heavy chains similar to, but not identical to, mu chains. Evidence now exists that surface IgM-like immunoglobulins of B lymphocytes and T lymphocytes activated to certain antigens can bind specifically to antigen. These observations suggest that surface immunoglobulin functions as a receptor for antigen on B cells and at least on some T cells. The mechanisms by which combination of antigen with surface immunoglobulin initiate differentiation remain to be determined.", "contents": "Lymphocyte surface immunoglobulins. Immunoglobulins have been isolated from the surface of B (bone marrow-derived) and T (thymus-derived) lymphocytes. Two types of membrane immunoglobulin occur on B lymphocytes; one type resembles the 200,000-dalton subunit of IgM, the second possesses a heavy chain electrophoretically distinct from mu chain and does not correspond to any of the known classes of mouse immunoglobulins. It might correspond to human sigma chain. T lymphocytes possess only one type of surface immunoglobulin. This molecule has a mass of approximately 200,000 daltons and contains light chains and heavy chains similar to, but not identical to, mu chains. Evidence now exists that surface IgM-like immunoglobulins of B lymphocytes and T lymphocytes activated to certain antigens can bind specifically to antigen. These observations suggest that surface immunoglobulin functions as a receptor for antigen on B cells and at least on some T cells. The mechanisms by which combination of antigen with surface immunoglobulin initiate differentiation remain to be determined."}
{"id": "PMID:1101379", "title": "Implication of Phlebotomus sand flies as vectors of bartonellosis and leishmaniasis as early as 1764.", "content": "A written account implicating Phlebotomus sand flies as vectors of Carrion's disease and cutaneous leishmaniasis in Peru was published by Cosme Bueno in 1764. Bueno's report precedes other publications implicating sand flies in the transmission of human pathogens by nearly a century and a half.", "contents": "Implication of Phlebotomus sand flies as vectors of bartonellosis and leishmaniasis as early as 1764. A written account implicating Phlebotomus sand flies as vectors of Carrion's disease and cutaneous leishmaniasis in Peru was published by Cosme Bueno in 1764. Bueno's report precedes other publications implicating sand flies in the transmission of human pathogens by nearly a century and a half."}
{"id": "PMID:1101380", "title": "Complement-mediated bactericidal system: evidence for a new pathway of complement action.", "content": "The early components of human complement (C1, C4, and C2) plus certain serum euglobulins will kill pathogenic strains of Shigella sonnei. Serum from patients with hereditary C3 deficiencies and specific antiserums to C3, C5, and C6 were utilized to demonstrate the absence of requirements for late-acting complement components in this unusual bactericidal system.", "contents": "Complement-mediated bactericidal system: evidence for a new pathway of complement action. The early components of human complement (C1, C4, and C2) plus certain serum euglobulins will kill pathogenic strains of Shigella sonnei. Serum from patients with hereditary C3 deficiencies and specific antiserums to C3, C5, and C6 were utilized to demonstrate the absence of requirements for late-acting complement components in this unusual bactericidal system."}
{"id": "PMID:1101385", "title": "Epidemiologic investigation of an outbreak of Shiga bacillus dysentery in an island population.", "content": "An epidemic of dysentery broke out in St. Martin island during May through July 1973. The epidemic was caused by Shigella dysenteriae type 1. The dysentery could not be controlled by conventional antibiotics and other antidysenteric drugs. The average attack rate was 32.9%. The age specific attack rate was highest in the age group 1-4 years (52.2%). The attack rates were higher in smaller families. The rates were not greatly different amongst people using different sources of water. The overall death rate was 2.1%. The overall infection-to-death rate was 6.4% but amongst children less than a year old, it was 41.1%. A common source outbreak was unlikely. The higher attack rate in smaller families suggested limited possibility of person to person spread. No particular water source could be implicated with higher attack rate. Flies may have played an active role in transmission.", "contents": "Epidemiologic investigation of an outbreak of Shiga bacillus dysentery in an island population. An epidemic of dysentery broke out in St. Martin island during May through July 1973. The epidemic was caused by Shigella dysenteriae type 1. The dysentery could not be controlled by conventional antibiotics and other antidysenteric drugs. The average attack rate was 32.9%. The age specific attack rate was highest in the age group 1-4 years (52.2%). The attack rates were higher in smaller families. The rates were not greatly different amongst people using different sources of water. The overall death rate was 2.1%. The overall infection-to-death rate was 6.4% but amongst children less than a year old, it was 41.1%. A common source outbreak was unlikely. The higher attack rate in smaller families suggested limited possibility of person to person spread. No particular water source could be implicated with higher attack rate. Flies may have played an active role in transmission."}
{"id": "PMID:1101386", "title": "Intravenous ketamine for postoperative analgesia.", "content": "Thirty-six consecutive patients who had undergone major surgery were carefully studied for 6 hours postoperatively in an attempt to evaluate the relative analgesic and respiratory-depressant effect of morphine, ketamine, and placebo administered by continuous intravenous infusion. Side-effects, vital signs and respiratory measurements were recorded hourly by a consultant physician. With regard to the dosage and method of administration, ketamine was shown to be less effective than morphine for the first 3 hours postoperatively, but equally effective subsequently, whereas the patients who received ketamine showed a greater progressive tendency for their respiratory parameters to improve with time. Recommendations regarding the postoperative control of pain are made, and the need for further study is noted.", "contents": "Intravenous ketamine for postoperative analgesia. Thirty-six consecutive patients who had undergone major surgery were carefully studied for 6 hours postoperatively in an attempt to evaluate the relative analgesic and respiratory-depressant effect of morphine, ketamine, and placebo administered by continuous intravenous infusion. Side-effects, vital signs and respiratory measurements were recorded hourly by a consultant physician. With regard to the dosage and method of administration, ketamine was shown to be less effective than morphine for the first 3 hours postoperatively, but equally effective subsequently, whereas the patients who received ketamine showed a greater progressive tendency for their respiratory parameters to improve with time. Recommendations regarding the postoperative control of pain are made, and the need for further study is noted."}
{"id": "PMID:1101387", "title": "A serological survey of toxoplasmosis in the Bloemfonetin area.", "content": "The IFA test revealed the presence of antibodies to Toxoplasma gondii in 10, 3% (62) of sera from 600 pregnant women. The incidence of antibodies varied from 8,0% in the Black population to 10,5% in White and 12,5% in Coloured women. In the Coloured and Black populations the highest incidence of antibodies was found in the 31-40-year age group, while in Whites this occurred in the 21-30-year age group. Twenty-eight sera also contained complement-fixing antibodies. The results are discussed in relation to the epidemiology, complications, diagnosis and treatment of toxoplasmosis.", "contents": "A serological survey of toxoplasmosis in the Bloemfonetin area. The IFA test revealed the presence of antibodies to Toxoplasma gondii in 10, 3% (62) of sera from 600 pregnant women. The incidence of antibodies varied from 8,0% in the Black population to 10,5% in White and 12,5% in Coloured women. In the Coloured and Black populations the highest incidence of antibodies was found in the 31-40-year age group, while in Whites this occurred in the 21-30-year age group. Twenty-eight sera also contained complement-fixing antibodies. The results are discussed in relation to the epidemiology, complications, diagnosis and treatment of toxoplasmosis."}
{"id": "PMID:1101388", "title": "Probable chloroquine-resistant Plasmodium falciparum malaria from Mozambique A case report.", "content": "A female patient with Plasmodium falciparum malaria apparently resistant to chloroquine is descirbed. She had recently returned from Mozambique, which may prove to be a new endemic are with resistant strains. The infection was successfully treated with quinine.", "contents": "Probable chloroquine-resistant Plasmodium falciparum malaria from Mozambique A case report. A female patient with Plasmodium falciparum malaria apparently resistant to chloroquine is descirbed. She had recently returned from Mozambique, which may prove to be a new endemic are with resistant strains. The infection was successfully treated with quinine."}
{"id": "PMID:1101389", "title": "A specific radio-immunoassay for gonadotrophin-releasing hormone.", "content": "A specific antiserum has been made to synthetic gonadotrophin-releasing hormone (GnRH) conjugated to keyhole limpet haemocyanin and appears to be directed against amino acids 6-8 of this decapeptide. This has allowed the development of a radio-immunoassay for GnRH sensitive to 5 picograms per tube. Although it is easily measurable in hypothalamic extracts, we have failed to detect GnRH in plasma and urine from normal subjects and menopausal women.", "contents": "A specific radio-immunoassay for gonadotrophin-releasing hormone. A specific antiserum has been made to synthetic gonadotrophin-releasing hormone (GnRH) conjugated to keyhole limpet haemocyanin and appears to be directed against amino acids 6-8 of this decapeptide. This has allowed the development of a radio-immunoassay for GnRH sensitive to 5 picograms per tube. Although it is easily measurable in hypothalamic extracts, we have failed to detect GnRH in plasma and urine from normal subjects and menopausal women."}
{"id": "PMID:1101390", "title": "History of mental health services in South Africa. Part VIII. Services for epileptics.", "content": "The development of services for epileptics in South Africa is reviewed. In the field of education, the history of the Jan Kriel school at Kuils River in the Western Cape is described. Treatment is provided at government mental hospitals, institutions for defectives, and general hospitals. The Epileptic Employment Association provides epileptics with work and opportunity to live a normal life. Information about epilepsy is provided by the South African National Epilepsy League, which also provides opportunities for employment.", "contents": "History of mental health services in South Africa. Part VIII. Services for epileptics. The development of services for epileptics in South Africa is reviewed. In the field of education, the history of the Jan Kriel school at Kuils River in the Western Cape is described. Treatment is provided at government mental hospitals, institutions for defectives, and general hospitals. The Epileptic Employment Association provides epileptics with work and opportunity to live a normal life. Information about epilepsy is provided by the South African National Epilepsy League, which also provides opportunities for employment."}
{"id": "PMID:1101392", "title": "Historical perspective of myocardial infarction.", "content": "The history of myocardial infarction ('coronary thrombosis'), a condition which was only recognised as a clinical entity in the 20th century, is reviewed up to the time of the introduction of the intensive coronary care units. Early observations of presentations, complications, diagnosis and treatment of the disease are emphasised. The increase in incidence of myocardial infarction, real or relative, is discussed and the remarkable variation shown in earlier reports on the mortality rate of myocardial infarction is explained. Many of the important principles which revolutionised the management of the disease through coronary care units, are evident in the earlier reports.", "contents": "Historical perspective of myocardial infarction. The history of myocardial infarction ('coronary thrombosis'), a condition which was only recognised as a clinical entity in the 20th century, is reviewed up to the time of the introduction of the intensive coronary care units. Early observations of presentations, complications, diagnosis and treatment of the disease are emphasised. The increase in incidence of myocardial infarction, real or relative, is discussed and the remarkable variation shown in earlier reports on the mortality rate of myocardial infarction is explained. Many of the important principles which revolutionised the management of the disease through coronary care units, are evident in the earlier reports."}
{"id": "PMID:1101396", "title": "Practical aspects of a cholera surveillance programme.", "content": "The recent invasion of southern Africa by cholera stimulated the establishment of a regional cholera reference laboratory and surveillance centre. This article describes its activities in general and details the methods employed in the isolation of Vibrio cholerae from sewage, water, seafoods, patients, and carriers. Importance is also attached to the isolation of NAG (non-agglutinable) V. cholerae and V. parahaemolyticus from certain categories of specimens. In recognition of the importance of regional co-operation in combating the problem of cholera, the collaboration of other countries on the subcontinent was invited, and the response is encouraging.", "contents": "Practical aspects of a cholera surveillance programme. The recent invasion of southern Africa by cholera stimulated the establishment of a regional cholera reference laboratory and surveillance centre. This article describes its activities in general and details the methods employed in the isolation of Vibrio cholerae from sewage, water, seafoods, patients, and carriers. Importance is also attached to the isolation of NAG (non-agglutinable) V. cholerae and V. parahaemolyticus from certain categories of specimens. In recognition of the importance of regional co-operation in combating the problem of cholera, the collaboration of other countries on the subcontinent was invited, and the response is encouraging."}
{"id": "PMID:1101397", "title": "History of mental health services in South Africa. Part IX. The protection and care of the feebleminded.", "content": "Interest in mental deficiency was at low ebb in the 19th century. The invention of intelligence tests in France and the publication of a book in the USA stimulated the Child Life Protection Society in Cape Town to call a meeting which led ultimately to the formation of the National Council for Mental Health. Details of early intelligence tests are given and the mental state of poor Whites is described. The reports of two committees on mental deficiency are discussed.", "contents": "History of mental health services in South Africa. Part IX. The protection and care of the feebleminded. Interest in mental deficiency was at low ebb in the 19th century. The invention of intelligence tests in France and the publication of a book in the USA stimulated the Child Life Protection Society in Cape Town to call a meeting which led ultimately to the formation of the National Council for Mental Health. Details of early intelligence tests are given and the mental state of poor Whites is described. The reports of two committees on mental deficiency are discussed."}
{"id": "PMID:1101400", "title": "Regional infusion chemotherapy for cancer of the head and neck.", "content": "The experience with intraarterial infusion chemotherapy in the 24 years since its development has established that it is not a substitute for conventional methods of management. Its prime indications are for palliation of advanced and recurrent cancer unsuitable for irradiation or surgery or as an adjunct to the latter. Several specific observations are relevant to its use in the head and neck region: 1. With currently available antineoplastic agents more than half of the neoplasms in this region can be made to regress. Epidermoid carcinomas, the most frequent tumor of the head and neck, as well as less frequent carcinomas and sarcomas can be affected. 2. Regressions are usually incomplete and brief, lasting but a few months, and despite complete tumor regression microscopic cancer usually persists. Occasionally, unpredictably, and for uncertain reasons a regression is complete and permanent. Small, well-differentiated and exophytic (verrucous) epidermoid cancers appear most liable to such a response. 3. Temporary or permanent neurologic damage is the most distressing morbidity associated with treatment. This hazard can be minimized by avoiding infusion of the internal carotid artery and protection of the patient from embolism of air or particulate matter through catheters. 4. The combined use of two or more chemotherapeutic agents is promising as a means for increasing the frequency and possibly the duration of tumor regressions. 5. The value of infusion as an adjunct to irradiation or surgery for potentially curable cancers is still unproved. Uncontrolled data suggest that local control of cancer may be improved, particularly if all three modalities are combined. A controlled clinical trial by experienced investigators could resolve this issue. 6. Although the arterial route increases the activity of some chemotherapeutic agents, significant progress appears to depend upon the development of drugs with greater specificity for neoplastic tissues. 7. In its present form, arterial infusion chemotherapy offers a brief period of palliation to many patients with cancer of the head and neck who are otherwise untreatable, and occasionally to such individuals a final chance for cure.", "contents": "Regional infusion chemotherapy for cancer of the head and neck. The experience with intraarterial infusion chemotherapy in the 24 years since its development has established that it is not a substitute for conventional methods of management. Its prime indications are for palliation of advanced and recurrent cancer unsuitable for irradiation or surgery or as an adjunct to the latter. Several specific observations are relevant to its use in the head and neck region: 1. With currently available antineoplastic agents more than half of the neoplasms in this region can be made to regress. Epidermoid carcinomas, the most frequent tumor of the head and neck, as well as less frequent carcinomas and sarcomas can be affected. 2. Regressions are usually incomplete and brief, lasting but a few months, and despite complete tumor regression microscopic cancer usually persists. Occasionally, unpredictably, and for uncertain reasons a regression is complete and permanent. Small, well-differentiated and exophytic (verrucous) epidermoid cancers appear most liable to such a response. 3. Temporary or permanent neurologic damage is the most distressing morbidity associated with treatment. This hazard can be minimized by avoiding infusion of the internal carotid artery and protection of the patient from embolism of air or particulate matter through catheters. 4. The combined use of two or more chemotherapeutic agents is promising as a means for increasing the frequency and possibly the duration of tumor regressions. 5. The value of infusion as an adjunct to irradiation or surgery for potentially curable cancers is still unproved. Uncontrolled data suggest that local control of cancer may be improved, particularly if all three modalities are combined. A controlled clinical trial by experienced investigators could resolve this issue. 6. Although the arterial route increases the activity of some chemotherapeutic agents, significant progress appears to depend upon the development of drugs with greater specificity for neoplastic tissues. 7. In its present form, arterial infusion chemotherapy offers a brief period of palliation to many patients with cancer of the head and neck who are otherwise untreatable, and occasionally to such individuals a final chance for cure."}
{"id": "PMID:1101403", "title": "Experimental stress ulcers: a review.", "content": "The restraint model and other models for the production of experimental stress ulcers have been reviewed. The mechanism of experimental stress ulcers appears to depend on an interaction between the presence of acid, changes in mucosal circulation, an increase in the excretion of glycoproteins in the mucus, and a decrease in mitotic activity of the mucosal lining of the stomach. Factors enhancing ulceration are cold, starvation, increased acidity, burns, reflux of bile, endotoxin, adrenalectomy, and hemorrhage. Factors inhibiting ulceration are vagotomy, anticholinergics, elemental diets, vitamin A, antacids, prevention of bile reflux, corticosteroids, epinephrine and norepinephrine, serotonin antagonists, and immediate replacement of blood loss with low molecular weight dextran. The role of sepsis is unclear and more work is needed in this area. Ulcers from intracranial injury are usually associated with the hypersecretion of gastric acid. Stimulation of the hypothalamus, directly or indirectly, with resultant vagal stimulation is thought to be the responsible mechanism for the increase in acid.", "contents": "Experimental stress ulcers: a review. The restraint model and other models for the production of experimental stress ulcers have been reviewed. The mechanism of experimental stress ulcers appears to depend on an interaction between the presence of acid, changes in mucosal circulation, an increase in the excretion of glycoproteins in the mucus, and a decrease in mitotic activity of the mucosal lining of the stomach. Factors enhancing ulceration are cold, starvation, increased acidity, burns, reflux of bile, endotoxin, adrenalectomy, and hemorrhage. Factors inhibiting ulceration are vagotomy, anticholinergics, elemental diets, vitamin A, antacids, prevention of bile reflux, corticosteroids, epinephrine and norepinephrine, serotonin antagonists, and immediate replacement of blood loss with low molecular weight dextran. The role of sepsis is unclear and more work is needed in this area. Ulcers from intracranial injury are usually associated with the hypersecretion of gastric acid. Stimulation of the hypothalamus, directly or indirectly, with resultant vagal stimulation is thought to be the responsible mechanism for the increase in acid."}
{"id": "PMID:1101404", "title": "Role of the endogenous gastrointestinal microflora in postoperative wound sepsis.", "content": "Most infections following gastrointestinal surgery are caused by the escape of endogenous microorganisms at the time of operation. A thorough understanding of the gastrointestinal microflora in health and disease is essential to the intelligent treatment of postoperative septic complications. The oral flora found under certain circumstances in the proximal gastrointestinal tract is usually penicillin sensitive. Both aerobic and anaerobic resident microflora of the distal ileum and colon are penicillin resistant; therefore, other antibiotic agents must be prescribed for successful treatment of sepsis following resection of these organs.", "contents": "Role of the endogenous gastrointestinal microflora in postoperative wound sepsis. Most infections following gastrointestinal surgery are caused by the escape of endogenous microorganisms at the time of operation. A thorough understanding of the gastrointestinal microflora in health and disease is essential to the intelligent treatment of postoperative septic complications. The oral flora found under certain circumstances in the proximal gastrointestinal tract is usually penicillin sensitive. Both aerobic and anaerobic resident microflora of the distal ileum and colon are penicillin resistant; therefore, other antibiotic agents must be prescribed for successful treatment of sepsis following resection of these organs."}
{"id": "PMID:1101410", "title": "Anesthesia and the immune system.", "content": "Anesthesia and operation may impair the immune system so that bacterial growth and tumor spread may occur more readily, and host response to transplanted tissue and allergenic substances may be altered. Suggestions are presented regarding the anesthetic management of patients at risk from infection or tumor spread.", "contents": "Anesthesia and the immune system. Anesthesia and operation may impair the immune system so that bacterial growth and tumor spread may occur more readily, and host response to transplanted tissue and allergenic substances may be altered. Suggestions are presented regarding the anesthetic management of patients at risk from infection or tumor spread."}
{"id": "PMID:1101409", "title": "Effects of anesthetics on splanchnic circulation and metabolism.", "content": "In the last 10 years intense interest in \"halothane hepatitis\" plus simpler and more reliable technology for measuring splanchnic blood flow have led to a fuller understanding of the splanchnic effects of anesthetics. For the first time, a rational choice of anesthetic technique can be made for the patient with splanchnic organ dysfunction.", "contents": "Effects of anesthetics on splanchnic circulation and metabolism. In the last 10 years intense interest in \"halothane hepatitis\" plus simpler and more reliable technology for measuring splanchnic blood flow have led to a fuller understanding of the splanchnic effects of anesthetics. For the first time, a rational choice of anesthetic technique can be made for the patient with splanchnic organ dysfunction."}
{"id": "PMID:1101411", "title": "The biochemical basis of anesthetic toxicity.", "content": "The inhalational anesthetics reversibly inhibit mitochondrial electron transport from NADH-linded substrates at the level of the enzyme NADH-dehydrogenase. The effect of this inhibition on ATP production is probably not the basis of the anesthetic state, as the level of high-energy phosphate in the brain does not decrease during anesthesia. Anesthetics, therefore, interfere with brain energy untilization, in addition to brain energy production. Inhibition by anesthetics of mitochondrial calcium uptake, which results from the inhibition of electron transport, may be an important anesthetic effect if it raises the intracellular calcium level. Other areas of investigation which have not been discussed here include the effects of anesthetics on glycolytic and other enzyme systems, ion fluxes, neurotransmitter synthesis and release, and on the structure of natural and artificial membranes. It is now appreciated that most of the inhalational anesthetics are metabolized by the endoplasmic reticulum of hepatocytes. The biotransformation of several anesthetics can be increased by drugs which induce hepatic microsomal enzymes. The organ toxicities of chloroform, trichloroethylene, fluroxene, methoxyflurane, and possibly of halothane and enflurane are related to their intermediary or final metabolites. It is of anesthetic drugs for an individual patient.", "contents": "The biochemical basis of anesthetic toxicity. The inhalational anesthetics reversibly inhibit mitochondrial electron transport from NADH-linded substrates at the level of the enzyme NADH-dehydrogenase. The effect of this inhibition on ATP production is probably not the basis of the anesthetic state, as the level of high-energy phosphate in the brain does not decrease during anesthesia. Anesthetics, therefore, interfere with brain energy untilization, in addition to brain energy production. Inhibition by anesthetics of mitochondrial calcium uptake, which results from the inhibition of electron transport, may be an important anesthetic effect if it raises the intracellular calcium level. Other areas of investigation which have not been discussed here include the effects of anesthetics on glycolytic and other enzyme systems, ion fluxes, neurotransmitter synthesis and release, and on the structure of natural and artificial membranes. It is now appreciated that most of the inhalational anesthetics are metabolized by the endoplasmic reticulum of hepatocytes. The biotransformation of several anesthetics can be increased by drugs which induce hepatic microsomal enzymes. The organ toxicities of chloroform, trichloroethylene, fluroxene, methoxyflurane, and possibly of halothane and enflurane are related to their intermediary or final metabolites. It is of anesthetic drugs for an individual patient."}
{"id": "PMID:1101413", "title": "Anesthetic management of patients with cardiac disease.", "content": "The course of anesthesia for patients with cardiac disease includes assessment and management of complications during the entire perioperative period. A thorough understanding of the pharmacology of anesthetic drugs and their interactions with medications taken by patients with heart disease is of utmost importance. The rapid changes in intraoperative circulatory status may often necessitate second-to-second invasive monitoring techniques to maintain stability. Finally, knowledge of complications occurring commonly in such patients allows the anesthesiologist to prepare ahead of time for corrective measures which may be lifesaving.", "contents": "Anesthetic management of patients with cardiac disease. The course of anesthesia for patients with cardiac disease includes assessment and management of complications during the entire perioperative period. A thorough understanding of the pharmacology of anesthetic drugs and their interactions with medications taken by patients with heart disease is of utmost importance. The rapid changes in intraoperative circulatory status may often necessitate second-to-second invasive monitoring techniques to maintain stability. Finally, knowledge of complications occurring commonly in such patients allows the anesthesiologist to prepare ahead of time for corrective measures which may be lifesaving."}
{"id": "PMID:1101415", "title": "Deliberate hypotension.", "content": "Deliberate hypotension can reduce major blood loss and indelicate operations can produce a drier field increasing the ease of surgery and the likelihood of a good result. The techniques used to induce hypotension can also  be used to avoid dangerous hypertension during and after surgery. These benefits must be weighed against the risks of inadequate perfusion: especially cerebral, myocardial, or renal. In previously normotensive patients these risks are minimal when the arterial pressure is held above 80 torr systolic, and may be acceptably small even at mean pressure of 50 to 60 torr. Previously hypertensive patients show signs of cerebral ischemia at higher pressures; they should probably not be subjected to deliberate hypotension, but they also can be harmed by severe hypertension which can be avioded by the proper use of hypotensive agents. For most situations a balanced technique is suitable: after a stable anesthetic level has been achieved using halothane or enflurane, hypotension can be induced with sodium nitroprusside or trimethaphan camsylate. Longer-acting agents such as pentolinium are sometimes desirable, but the shorter-acting agents are easier to control. Careful monitoring with observation of intra-arterial pressure, electroencephalogram, electrocardiogram, and determination arterial blood gas tensions is likely to make for safer conduct. Close postoperative observation is essential. With careful preparation and monitoring deliberate hypotension can be a safe technique for reducing blood loss or facilitating delicate procedures.", "contents": "Deliberate hypotension. Deliberate hypotension can reduce major blood loss and indelicate operations can produce a drier field increasing the ease of surgery and the likelihood of a good result. The techniques used to induce hypotension can also  be used to avoid dangerous hypertension during and after surgery. These benefits must be weighed against the risks of inadequate perfusion: especially cerebral, myocardial, or renal. In previously normotensive patients these risks are minimal when the arterial pressure is held above 80 torr systolic, and may be acceptably small even at mean pressure of 50 to 60 torr. Previously hypertensive patients show signs of cerebral ischemia at higher pressures; they should probably not be subjected to deliberate hypotension, but they also can be harmed by severe hypertension which can be avioded by the proper use of hypotensive agents. For most situations a balanced technique is suitable: after a stable anesthetic level has been achieved using halothane or enflurane, hypotension can be induced with sodium nitroprusside or trimethaphan camsylate. Longer-acting agents such as pentolinium are sometimes desirable, but the shorter-acting agents are easier to control. Careful monitoring with observation of intra-arterial pressure, electroencephalogram, electrocardiogram, and determination arterial blood gas tensions is likely to make for safer conduct. Close postoperative observation is essential. With careful preparation and monitoring deliberate hypotension can be a safe technique for reducing blood loss or facilitating delicate procedures."}
{"id": "PMID:1101417", "title": "Trace anesthetic exposure. Consequences and control.", "content": "Epidemiologic and animal studies indicate that chronic exposure to anesthetic gases may consitute a potential health hazard. This is suggested by a higher incidence of spontaneous abortion among female operating room personnel, congenital anomalies in the offspring of operating room personnel, and neoplasia of the reticuloendothelial system. Human data show that performance can be compromised during exposure to anesthetic traces. A direct cause and effect relationship between anesthetic exposure and the alleged hazards will require further prospective studies. Such a study is being planned by the Ad Hoc Committee of the American Society of Anesthesiologists. In the interim, monitoring programs should be established to define adequate clearances of the anesthetic agents. Minimum standards and recommended procedures should be established regarding operating room ventilation and design. The use of effective scavengers and anesthetic techniques not requiring the use of inhalational agents should be encouraged. In this way a clear distinction may be defined by the prosepective study planned by the American Society of Anesthesiologists in 1978.", "contents": "Trace anesthetic exposure. Consequences and control. Epidemiologic and animal studies indicate that chronic exposure to anesthetic gases may consitute a potential health hazard. This is suggested by a higher incidence of spontaneous abortion among female operating room personnel, congenital anomalies in the offspring of operating room personnel, and neoplasia of the reticuloendothelial system. Human data show that performance can be compromised during exposure to anesthetic traces. A direct cause and effect relationship between anesthetic exposure and the alleged hazards will require further prospective studies. Such a study is being planned by the Ad Hoc Committee of the American Society of Anesthesiologists. In the interim, monitoring programs should be established to define adequate clearances of the anesthetic agents. Minimum standards and recommended procedures should be established regarding operating room ventilation and design. The use of effective scavengers and anesthetic techniques not requiring the use of inhalational agents should be encouraged. In this way a clear distinction may be defined by the prosepective study planned by the American Society of Anesthesiologists in 1978."}
{"id": "PMID:1101419", "title": "Hypothermic storage of the canine kidney for 48 hours in a low chloride solution.", "content": "In studies of 52 dogs, kidneys were stored at zero degrees C. using a low chloride hypertonic intracellular solution. When stored for 24 hours, one-half of the kidneys resumed normal function upon reimplantation. With storage for 48 hours using a brief washout at 24 hours, up to 38 per cent of the reimplanted kidneys provided life sustaining function without azotemia.", "contents": "Hypothermic storage of the canine kidney for 48 hours in a low chloride solution. In studies of 52 dogs, kidneys were stored at zero degrees C. using a low chloride hypertonic intracellular solution. When stored for 24 hours, one-half of the kidneys resumed normal function upon reimplantation. With storage for 48 hours using a brief washout at 24 hours, up to 38 per cent of the reimplanted kidneys provided life sustaining function without azotemia."}
{"id": "PMID:1101420", "title": "The role of the nurse epidemiologist in infection control and continuing education.", "content": "A university affiliated Veterans Administration Hospital had an increase in the number of serratia isolates. An epidemiologic investigation, including personnel and environmental prevalence study, helped correct errors in technique, management and judgment. A nurse epidemiologist prevented a cluster of infections from becoming an epidemic.", "contents": "The role of the nurse epidemiologist in infection control and continuing education. A university affiliated Veterans Administration Hospital had an increase in the number of serratia isolates. An epidemiologic investigation, including personnel and environmental prevalence study, helped correct errors in technique, management and judgment. A nurse epidemiologist prevented a cluster of infections from becoming an epidemic."}
{"id": "PMID:1101421", "title": "Simultaneous study of gastric and pancreatic secretion in a preparation perfused ex vivo.", "content": "A canine gastroduodenopancreatic block was isolated and perfused ex vivo with homologous blood. This preparation allowed a simultaneous collection of gastric and pancreatic secretion under various conditions of stimulation. To stimulate gastric secretion, pentagastrin, bethanechol chloride or histamine was infused intra-arterially during a six to eight hour period, or both vagi were electrically stimulated. To stimulate pancreatic secretion, secretin was infused by the same method. Secretin infusion inhibited gastric juice volume and acid output by stimulated stomachs and induced an increase in pepsin concentration. Secretin infusion also resulted in a significant increase of flow of pancreatic juice and in both concentration and output of pancreatic secretion of bicarbonate. Arterial perfusion pressure and peripheral vascular resistance of this ex vivo preparation were significantly reduced by secretin. The method described permits the simultaneous study of the secretory function of the stomach and pancreas as well as the evaluation of biochemical changes of perfusion blood and hemodynamic changes in the circuit. Easy accessibility of the vessels and vagus branches also allows direct infusion of drugs and placement of stimulation electrodes.", "contents": "Simultaneous study of gastric and pancreatic secretion in a preparation perfused ex vivo. A canine gastroduodenopancreatic block was isolated and perfused ex vivo with homologous blood. This preparation allowed a simultaneous collection of gastric and pancreatic secretion under various conditions of stimulation. To stimulate gastric secretion, pentagastrin, bethanechol chloride or histamine was infused intra-arterially during a six to eight hour period, or both vagi were electrically stimulated. To stimulate pancreatic secretion, secretin was infused by the same method. Secretin infusion inhibited gastric juice volume and acid output by stimulated stomachs and induced an increase in pepsin concentration. Secretin infusion also resulted in a significant increase of flow of pancreatic juice and in both concentration and output of pancreatic secretion of bicarbonate. Arterial perfusion pressure and peripheral vascular resistance of this ex vivo preparation were significantly reduced by secretin. The method described permits the simultaneous study of the secretory function of the stomach and pancreas as well as the evaluation of biochemical changes of perfusion blood and hemodynamic changes in the circuit. Easy accessibility of the vessels and vagus branches also allows direct infusion of drugs and placement of stimulation electrodes."}
{"id": "PMID:1101423", "title": "Vascular access for hemodialysis.", "content": "The subcutaneous arteriovenous fistula has become the method of choice for vascular access for chronic hemodialysis in the vast majority of dialysis centers in the United States and abroad. The Silastic cannula external arteriovenous shunt is an acceptable alternative and is the access of choice for treatment of acute renal failure or drug overdose. Surgeons dealing with these patients will have to familiarize themselves with both procedures, as well as with the various modifications currently in use in order to offer the best procedure for the particular patient and to make dialysis possible for all patients who need it.", "contents": "Vascular access for hemodialysis. The subcutaneous arteriovenous fistula has become the method of choice for vascular access for chronic hemodialysis in the vast majority of dialysis centers in the United States and abroad. The Silastic cannula external arteriovenous shunt is an acceptable alternative and is the access of choice for treatment of acute renal failure or drug overdose. Surgeons dealing with these patients will have to familiarize themselves with both procedures, as well as with the various modifications currently in use in order to offer the best procedure for the particular patient and to make dialysis possible for all patients who need it."}
{"id": "PMID:1101429", "title": "Post-transplant function of cadaver kidneys preserved with plasma perfusate containing antidonor antibody.", "content": "Two cadaver kidneys were preserved by the perfusion method with AB cryoprecipitated plasma which contained antidonor lymphocytotoxic antibody. The kidneys were transplanted subsequently and no demonstrable immunologic damage to the kidneys was observed in the preservation and post-transplantation periods. In one patient renal function was immediate, and function has remained satisfactory in the 3 month follow-up period. The other patient had an initial period (2 weeks) of acute tubular necrosis, but renal function became satisfactory and the patient's condition has remained stable in the 3 month follow-up period. The significance of antidonor lymphocytotoxic antibody in the plasma perfusate on subsequent kidney function has been discussed.", "contents": "Post-transplant function of cadaver kidneys preserved with plasma perfusate containing antidonor antibody. Two cadaver kidneys were preserved by the perfusion method with AB cryoprecipitated plasma which contained antidonor lymphocytotoxic antibody. The kidneys were transplanted subsequently and no demonstrable immunologic damage to the kidneys was observed in the preservation and post-transplantation periods. In one patient renal function was immediate, and function has remained satisfactory in the 3 month follow-up period. The other patient had an initial period (2 weeks) of acute tubular necrosis, but renal function became satisfactory and the patient's condition has remained stable in the 3 month follow-up period. The significance of antidonor lymphocytotoxic antibody in the plasma perfusate on subsequent kidney function has been discussed."}
{"id": "PMID:1101430", "title": "Tissue levels of prostaglandin E following heterotopic rat heart allografting.", "content": "Heterotopic rat heart allografts were performed in 35 rats. At intervals after transplantation, rats were killed and tissue concentrations of immunoassayable prostaglandin E (PGE) were measured in the native heart, transplanted heart, spleen, and thymus. A significant increase in allograft PGE activity was observed at days 5 through 7, coinciding with the onset of the rejection reaction. These studies implicate PGE in the host response to allografting, but further studies are needed to determine what (if any) role prostaglandins play in the rejection reaction.", "contents": "Tissue levels of prostaglandin E following heterotopic rat heart allografting. Heterotopic rat heart allografts were performed in 35 rats. At intervals after transplantation, rats were killed and tissue concentrations of immunoassayable prostaglandin E (PGE) were measured in the native heart, transplanted heart, spleen, and thymus. A significant increase in allograft PGE activity was observed at days 5 through 7, coinciding with the onset of the rejection reaction. These studies implicate PGE in the host response to allografting, but further studies are needed to determine what (if any) role prostaglandins play in the rejection reaction."}
{"id": "PMID:1101431", "title": "Orthotopic liver transplantation: an experimental study on mechanisms of hemorrhagic diathesis and thrombosis.", "content": "The hemorrhagic diathesis after transplantation of preserved livers generally is attributed to intravascular coagulation, whereas postoperative \"hypercoagulability\" of the blood is considered the main cause of thrombosis of the hepatic artery anastomosis. Since our preliminary studies, however, suggested other mechanisms, parameters of coagulation, fibrinolysis, and platelet function were prospectively studied before and after 28 orthotopic liver transplantations, with and without preservation, in dogs and pigs. In addition, the arterial anastomoses were evaluated routinely by angiography and, after removal at reintervention or autopsy, inspected for thrombosis and tested for fibrinolytic activity and fibrin deposition. Concerning the hemorrhagic diathesis, prolongation of bleeding time without concomitant thrombocytopenia was the main abnormality found and occurred only in the nine dogs with liver transplants previously preserved for 3 to 6 hours. As only two of the nine dogs had postoperative hemorrhage of clinical significance, we consider surgically imperfect hemostasis facilitated by an acquired platelet dysfunction the principal cause of hemorrhage. Thrombosis of the arterial anastomosis was found in 38 percent of animals in which an end-to-end anastomosis was made but was not encountered with celiac artery-aorta anastomoses. Local factors due to surgical technique therefore appear most important in the pathogenesis of thrombosis of the hepatic artery anastomosis, although the postoperative hyperfibrinogenemia and diminished local and systemic fibrinolytic activity may contribute as well.", "contents": "Orthotopic liver transplantation: an experimental study on mechanisms of hemorrhagic diathesis and thrombosis. The hemorrhagic diathesis after transplantation of preserved livers generally is attributed to intravascular coagulation, whereas postoperative \"hypercoagulability\" of the blood is considered the main cause of thrombosis of the hepatic artery anastomosis. Since our preliminary studies, however, suggested other mechanisms, parameters of coagulation, fibrinolysis, and platelet function were prospectively studied before and after 28 orthotopic liver transplantations, with and without preservation, in dogs and pigs. In addition, the arterial anastomoses were evaluated routinely by angiography and, after removal at reintervention or autopsy, inspected for thrombosis and tested for fibrinolytic activity and fibrin deposition. Concerning the hemorrhagic diathesis, prolongation of bleeding time without concomitant thrombocytopenia was the main abnormality found and occurred only in the nine dogs with liver transplants previously preserved for 3 to 6 hours. As only two of the nine dogs had postoperative hemorrhage of clinical significance, we consider surgically imperfect hemostasis facilitated by an acquired platelet dysfunction the principal cause of hemorrhage. Thrombosis of the arterial anastomosis was found in 38 percent of animals in which an end-to-end anastomosis was made but was not encountered with celiac artery-aorta anastomoses. Local factors due to surgical technique therefore appear most important in the pathogenesis of thrombosis of the hepatic artery anastomosis, although the postoperative hyperfibrinogenemia and diminished local and systemic fibrinolytic activity may contribute as well."}
{"id": "PMID:1101433", "title": "The composition of massive lesions in coal miners.", "content": "The nature of the material forming the massive lesions in the lungs of coal workers has never been demonstrated. The concept that it was in fact massive fibrosis, implying that it consisted of collagen impregnated with coal dust, has been challenged only during the last ten years. It was agreed that the best chance of obtaining more definite information was from a combined study of the biochemical, pathological, ultrastructural, and immunological features of a number of lungs containing these lesions. Six cases which were found to contain suitable material were studied. The preliminary results obtained suggest that collagen is present in the capsule of these lesions but that at the centre it is replaced by another insoluble protein or proteins which is probably stabilized by some form of cross-linking. This protein complex accounts for one-third of the weight of the lesions, the remaining two-thirds consisting of approximately equal amounts of mineral dusts and calcium phosphate. Serum proteins were also observed but their association with the lesions has yet to be determined.", "contents": "The composition of massive lesions in coal miners. The nature of the material forming the massive lesions in the lungs of coal workers has never been demonstrated. The concept that it was in fact massive fibrosis, implying that it consisted of collagen impregnated with coal dust, has been challenged only during the last ten years. It was agreed that the best chance of obtaining more definite information was from a combined study of the biochemical, pathological, ultrastructural, and immunological features of a number of lungs containing these lesions. Six cases which were found to contain suitable material were studied. The preliminary results obtained suggest that collagen is present in the capsule of these lesions but that at the centre it is replaced by another insoluble protein or proteins which is probably stabilized by some form of cross-linking. This protein complex accounts for one-third of the weight of the lesions, the remaining two-thirds consisting of approximately equal amounts of mineral dusts and calcium phosphate. Serum proteins were also observed but their association with the lesions has yet to be determined."}
{"id": "PMID:1101442", "title": "[Effect of the diluent on the aerobic bacterial count in raw minced meat and similar meats (author's transl)].", "content": "A report on the changes in the aerobic bacterial count in commercial minced meat, which appear when samples, decimally diluted with 0.85 per cent sodium chloride or with 0.85 per cent sodium chloride plus peptone (peptone physiological saline solution, p.ph.s.s.) are stored at room temperature for some time. When the higher dilutions (10(5) to 10(7) inclusive) were stored for periods of thirty minutes and more, significantly higher counts were recorded on using p.ph.s.s. and significantly lower counts were observed on using sodium chloride. Significantly higher counts were produced, however, when the two diluents were used following storage of the lowest dilution (10(1)) for a similar period. The dilutions have to be mixed again after each period of storage. The presence of \"sublethally injured\" bacteria in commercial minced meat is discussed. These bacteria may recover when a diluent having a sufficiently protective effect is used. Substances from the natural substrate are probably still active at the lowest dilution. A number of the injured bacteria which fail to multiply when the pour-plate method is used, will probably be able to produce colonies when the streak-plate method is adopted as more oxygen will be present in this case. Even when a rapid procedure is used in examining the samples (mixed agar method), the average number of colonies produced on using sodium chloride will be significantly smaller than that produced when p.ph.s.s. is used.", "contents": "[Effect of the diluent on the aerobic bacterial count in raw minced meat and similar meats (author's transl)]. A report on the changes in the aerobic bacterial count in commercial minced meat, which appear when samples, decimally diluted with 0.85 per cent sodium chloride or with 0.85 per cent sodium chloride plus peptone (peptone physiological saline solution, p.ph.s.s.) are stored at room temperature for some time. When the higher dilutions (10(5) to 10(7) inclusive) were stored for periods of thirty minutes and more, significantly higher counts were recorded on using p.ph.s.s. and significantly lower counts were observed on using sodium chloride. Significantly higher counts were produced, however, when the two diluents were used following storage of the lowest dilution (10(1)) for a similar period. The dilutions have to be mixed again after each period of storage. The presence of \"sublethally injured\" bacteria in commercial minced meat is discussed. These bacteria may recover when a diluent having a sufficiently protective effect is used. Substances from the natural substrate are probably still active at the lowest dilution. A number of the injured bacteria which fail to multiply when the pour-plate method is used, will probably be able to produce colonies when the streak-plate method is adopted as more oxygen will be present in this case. Even when a rapid procedure is used in examining the samples (mixed agar method), the average number of colonies produced on using sodium chloride will be significantly smaller than that produced when p.ph.s.s. is used."}
{"id": "PMID:1101443", "title": "Leptospirae of serotype lora of the serogroup Australis isolated for the first time from swine in the Netherlands.", "content": "Leptospirosis in swine, caused by serotype lora (serogroup Australis) was detected both serologically and by culture. The most important symptoms consisted in abortion during the final month of gestation and the birth of dead or not viable piglets.", "contents": "Leptospirae of serotype lora of the serogroup Australis isolated for the first time from swine in the Netherlands. Leptospirosis in swine, caused by serotype lora (serogroup Australis) was detected both serologically and by culture. The most important symptoms consisted in abortion during the final month of gestation and the birth of dead or not viable piglets."}
{"id": "PMID:1101444", "title": "A new CoDNA-ase spot plate method for the detection of coagulase and DNA-ase activity of staphylococci.", "content": "A new method for the determination of the coagulase and DNA-ase activity of staphylococci is described. The method enables the analyst to test large numbers of strains for this microorganism without the loss of time involved in the conventional technique.", "contents": "A new CoDNA-ase spot plate method for the detection of coagulase and DNA-ase activity of staphylococci. A new method for the determination of the coagulase and DNA-ase activity of staphylococci is described. The method enables the analyst to test large numbers of strains for this microorganism without the loss of time involved in the conventional technique."}
{"id": "PMID:1101447", "title": "Immune responses in Mycoplasma pneumoniae infections.", "content": "All children with Mycoplasma pneumoniae infection showed a significant response in IgG immunofluorescent (IMF) antibody titers, which were similar to that of complement-fixing (CF) antibody titers. However, there was a certain degree of variation in IgM IMF antibody response and its persistence. This variation also occurred in cases with pre-existing specific antibodies and good CF and IgG IMF antibody titers. Thus, it was considered that a reinfection with M. pneumoniae might not have an important effect upon the individual variation in IgM immune response, and that after a secondary immune response with M. pneumoniae infection with specific IgM antibody could be detected in some patients.", "contents": "Immune responses in Mycoplasma pneumoniae infections. All children with Mycoplasma pneumoniae infection showed a significant response in IgG immunofluorescent (IMF) antibody titers, which were similar to that of complement-fixing (CF) antibody titers. However, there was a certain degree of variation in IgM IMF antibody response and its persistence. This variation also occurred in cases with pre-existing specific antibodies and good CF and IgG IMF antibody titers. Thus, it was considered that a reinfection with M. pneumoniae might not have an important effect upon the individual variation in IgM immune response, and that after a secondary immune response with M. pneumoniae infection with specific IgM antibody could be detected in some patients."}
{"id": "PMID:1101450", "title": "[The bonding of enamel and dentin by means of adhesive materials. 4. Possible use of noracryl 100 in orthodontics].", "content": "The present paper deals with first personal experience. The indications concern orthodontic space elimination and movement of single teeth in the anterior region. This method is a true rationalization in orthodontic practice.", "contents": "[The bonding of enamel and dentin by means of adhesive materials. 4. Possible use of noracryl 100 in orthodontics]. The present paper deals with first personal experience. The indications concern orthodontic space elimination and movement of single teeth in the anterior region. This method is a true rationalization in orthodontic practice."}
{"id": "PMID:1101451", "title": "[The model casting method for inlays and bridgework].", "content": "The authors deal with the advantages and disadvantages of the direct and the indirect method of inlay making and describe the precision casting technique for inlays and bridges. Furthermore, they present the telescopic tray which has been developed in the Rostock Clinic. This tray permits to apply the impression material under increased pressure. The use of the compound embedding material and dental alloys indicated allows to obtain good treatment results in a rational manner.", "contents": "[The model casting method for inlays and bridgework]. The authors deal with the advantages and disadvantages of the direct and the indirect method of inlay making and describe the precision casting technique for inlays and bridges. Furthermore, they present the telescopic tray which has been developed in the Rostock Clinic. This tray permits to apply the impression material under increased pressure. The use of the compound embedding material and dental alloys indicated allows to obtain good treatment results in a rational manner."}
{"id": "PMID:1101452", "title": "[Stomatological morbidity study--Berlin 1972. 4. State of prosthetic-stomatologic care and the need for it].", "content": "In the course of a representative morbidity study in the capital of Berlin of 1972, it was found that almost 56% of the adults needed prosthodontic care. Only nearly half of them had dental prostheses of sufficient quality, one fourth had insufficient dental prostheses, and one fourth were untreated.", "contents": "[Stomatological morbidity study--Berlin 1972. 4. State of prosthetic-stomatologic care and the need for it]. In the course of a representative morbidity study in the capital of Berlin of 1972, it was found that almost 56% of the adults needed prosthodontic care. Only nearly half of them had dental prostheses of sufficient quality, one fourth had insufficient dental prostheses, and one fourth were untreated."}
{"id": "PMID:1101454", "title": "[The state of cytology in dentistry--a review].", "content": "Cytological examinations have been introduced into stomatology relatively late though the material is easy to collect and the techniques used are inexpensive. At present, cytological examinations may be employed for the following purposes: 1. Differential diagnosis of bullous lesions of the oral mucosa. 2. Supervision of inflammatory processes of the periodontium and related surgical interventions. 3. Detection of malignant and premalignant processes in the oral cavity and its environments. Apart from the well-known staining procedures, the acridine orange fluorescence method and recently, the cytometric evaluation of historadiographic techniques are used. Especially in the diagnosis of tumours, cytology is only an adjunct however worthwile.", "contents": "[The state of cytology in dentistry--a review]. Cytological examinations have been introduced into stomatology relatively late though the material is easy to collect and the techniques used are inexpensive. At present, cytological examinations may be employed for the following purposes: 1. Differential diagnosis of bullous lesions of the oral mucosa. 2. Supervision of inflammatory processes of the periodontium and related surgical interventions. 3. Detection of malignant and premalignant processes in the oral cavity and its environments. Apart from the well-known staining procedures, the acridine orange fluorescence method and recently, the cytometric evaluation of historadiographic techniques are used. Especially in the diagnosis of tumours, cytology is only an adjunct however worthwile."}
{"id": "PMID:1101455", "title": "[Edlan-Mejchar's method as a preprosthetic surgical measure for the stabilization of total mandibular prostheses. Report on first experiences].", "content": "In the present 1-year study, the method inaugurated by Edlan and Mejchar as a \"periodontal-preventive operation intended for deepening the lower oral vestibule\" was used as a preprosthetic-surgical measure. The operating technique is described in detail and the suitability of this method is proved by the results from 13 patients. It is a very recommendable method for deepening the lower oral vestibule.", "contents": "[Edlan-Mejchar's method as a preprosthetic surgical measure for the stabilization of total mandibular prostheses. Report on first experiences]. In the present 1-year study, the method inaugurated by Edlan and Mejchar as a \"periodontal-preventive operation intended for deepening the lower oral vestibule\" was used as a preprosthetic-surgical measure. The operating technique is described in detail and the suitability of this method is proved by the results from 13 patients. It is a very recommendable method for deepening the lower oral vestibule."}
{"id": "PMID:1101458", "title": "Neutralizing antibody in Rodent Malaria.", "content": "Small numbers of rat erythrocytes infected with viable P. berghei, when inoculated into susceptible rats together with hyperimmune rat serum (HIS), are fully neutralized. Serum from convalescent rats delays the onset of patency but does not neutralize. The neutralizing efficiency of HIS rises in proportion to the number of successive reinoculations of hyperimmune rats. In contrast, mice inoculated with parasites together with either HIS or normal rat serum succumbed to the disease at the same time after inoculation. Neutralization in rats occurs in vivo and is completed within 22 hours of inoculation. Much larger amounts of HIS are needed to achieve neutralization in splenectomized recipients than in intact rats. The action of HIS is dose-dependent. Thus, the degree of suppression of parasitaemia is proportional to the dose of HIS, while the mortality rate is inversely proportional to the dose. Suboptimal doses may even enhance the infection of recipient rats. The ability to produce neutralizing antibody is dissimilar in two strains of rat. Thus, the outbred Sabra strain produces neutralizing HIS, while the inbred Lewis rat is incapable of producing perceptible neutralizing antibody in our experimental model.", "contents": "Neutralizing antibody in Rodent Malaria. Small numbers of rat erythrocytes infected with viable P. berghei, when inoculated into susceptible rats together with hyperimmune rat serum (HIS), are fully neutralized. Serum from convalescent rats delays the onset of patency but does not neutralize. The neutralizing efficiency of HIS rises in proportion to the number of successive reinoculations of hyperimmune rats. In contrast, mice inoculated with parasites together with either HIS or normal rat serum succumbed to the disease at the same time after inoculation. Neutralization in rats occurs in vivo and is completed within 22 hours of inoculation. Much larger amounts of HIS are needed to achieve neutralization in splenectomized recipients than in intact rats. The action of HIS is dose-dependent. Thus, the degree of suppression of parasitaemia is proportional to the dose of HIS, while the mortality rate is inversely proportional to the dose. Suboptimal doses may even enhance the infection of recipient rats. The ability to produce neutralizing antibody is dissimilar in two strains of rat. Thus, the outbred Sabra strain produces neutralizing HIS, while the inbred Lewis rat is incapable of producing perceptible neutralizing antibody in our experimental model."}
{"id": "PMID:1101456", "title": "The contribution of computerized axial tomography to the diagnosis of cerebellar and pontine hematomas.", "content": "Case histories are reported of seven patients in whom the diagnosis of cerebellar (four) or pontine hemorrhage (three) was made by computerized axial tomography (CT). In all of the patients except two the diagnosis, established on the basis of a space-occupying high absorption lesion, was proved by either operation or autopical for an intracerebral hematoma, i.e., the high absorption zone was transformed into an area of reduced density. It is concluded that CT is a highly reliable method for the diagnosis and location of posterior fossa hematomas. A CT syndrome, observed in four of the seven cases, is described consisting of a hydrocephalus with the tips of the posterior horns being filled with blood. This seems to be specific for a posterior fossa hematoma having penetrated into the fourth ventricle.", "contents": "The contribution of computerized axial tomography to the diagnosis of cerebellar and pontine hematomas. Case histories are reported of seven patients in whom the diagnosis of cerebellar (four) or pontine hemorrhage (three) was made by computerized axial tomography (CT). In all of the patients except two the diagnosis, established on the basis of a space-occupying high absorption lesion, was proved by either operation or autopical for an intracerebral hematoma, i.e., the high absorption zone was transformed into an area of reduced density. It is concluded that CT is a highly reliable method for the diagnosis and location of posterior fossa hematomas. A CT syndrome, observed in four of the seven cases, is described consisting of a hydrocephalus with the tips of the posterior horns being filled with blood. This seems to be specific for a posterior fossa hematoma having penetrated into the fourth ventricle."}
{"id": "PMID:1101457", "title": "Effect of cyclandelate on dementia.", "content": "Cyclandelate, a vasodilator, was administered to 24 patients with dementia. The dementia in these patients was presumed to be due to cerebral ischemia caused by atherosclerosis in cerebral vessels after other possible causes were ruled out. In a double-blind, cross-over study, patients received 200 mg of cyclandelate four times daily for six weeks and a placebo for six weeks. Six psychological tests, which reflect various aspects of higher cortical ability, were used to evaluate the effect of cyclandelate on the dementia. Cyclandelate was found to be no more effective than placebo in improving higher cortical function in these demented patients.", "contents": "Effect of cyclandelate on dementia. Cyclandelate, a vasodilator, was administered to 24 patients with dementia. The dementia in these patients was presumed to be due to cerebral ischemia caused by atherosclerosis in cerebral vessels after other possible causes were ruled out. In a double-blind, cross-over study, patients received 200 mg of cyclandelate four times daily for six weeks and a placebo for six weeks. Six psychological tests, which reflect various aspects of higher cortical ability, were used to evaluate the effect of cyclandelate on the dementia. Cyclandelate was found to be no more effective than placebo in improving higher cortical function in these demented patients."}
{"id": "PMID:1101464", "title": "Comparison of a 9-phenanthrene methanol (WR33063), a 4-quinoline methanol (WR30090), and quinine for falciparum malaria in Thailand.", "content": "Quinine was compared with a 9-phenanthrene methanol (WR33063) and a 4-quinoline methanol (WR30090) for the treatment of 207 patients with falciparum malaria in Southeast Thailand. Quinine eradicated parasitaemia (average 70 hours) more rapidly than either WR30090 (72 hours) or WR33063 (77 hours). But WR33063 had a higher cure rate (92%) than WR30090 (86%) or quinine (85%). The mean duration of fever and of parasitaemia were combined with the failure rate to form an arbitrary efficacy index. Using this concept WR33063 was the most effective drug. The recrudescence rate correlated with the degree and duration of parasitaemia and with the duration of fever. WR33063 was the least toxic drug. Side effects associated with WR30090 appeared to be headache, backache and urticaria. Quinine was the most toxic drug. All 3 drugs were inconvenient in having to be administered every 8 hours for 6 days. One patient did not respond to oral quinine but did respond to an intravenous quinine infusion (IVQ). A \"Medication Ward Round\" was perfected during the study and comprised sequential history, drug administration, physical examination, dose notation and patient observation. Falciparum nephrosis was diagnosed in one patient.", "contents": "Comparison of a 9-phenanthrene methanol (WR33063), a 4-quinoline methanol (WR30090), and quinine for falciparum malaria in Thailand. Quinine was compared with a 9-phenanthrene methanol (WR33063) and a 4-quinoline methanol (WR30090) for the treatment of 207 patients with falciparum malaria in Southeast Thailand. Quinine eradicated parasitaemia (average 70 hours) more rapidly than either WR30090 (72 hours) or WR33063 (77 hours). But WR33063 had a higher cure rate (92%) than WR30090 (86%) or quinine (85%). The mean duration of fever and of parasitaemia were combined with the failure rate to form an arbitrary efficacy index. Using this concept WR33063 was the most effective drug. The recrudescence rate correlated with the degree and duration of parasitaemia and with the duration of fever. WR33063 was the least toxic drug. Side effects associated with WR30090 appeared to be headache, backache and urticaria. Quinine was the most toxic drug. All 3 drugs were inconvenient in having to be administered every 8 hours for 6 days. One patient did not respond to oral quinine but did respond to an intravenous quinine infusion (IVQ). A \"Medication Ward Round\" was perfected during the study and comprised sequential history, drug administration, physical examination, dose notation and patient observation. Falciparum nephrosis was diagnosed in one patient."}
{"id": "PMID:1101469", "title": "A disquisition on suppressor T cells.", "content": "The main points that  I have put forth are that: (1) suppressor T cell activity cannot be explained as simply being too much help; (2) feedback signals from target cells are of crucial importance in determining and maintaining the activity of suppressor T cells; (3) whenever T cells are triggered by antigen, suppression occurs. Immune responses only occur when countermanding signals are also generated. Both intrinsic and extrinsic adjuvanticity is the operational production of countermanding signals; (4) memory T cells are qualitatively different from normal T cells in their sensitivity to feedback signals and also in their susceptibility to suppression; (5) mature thymus dependent B cells cannot be rendered tolerant by the direct action of antigen, while immature and thymus independent B cells can; (6) the mechanism of suppression induced by exogenously administered antigens and that by normal differentiation products (i.e.: GVH; allotypes), is different; (7) generation of suppressor cells requires or results from complex interactions between subpopulations of cells, making it impossible under present conditions to determine which cell is doing what and to which; (8) further work is required before a full understanding of the importance, mechanism of action and other aspects of suppressor T cell function can be fully understood.", "contents": "A disquisition on suppressor T cells. The main points that  I have put forth are that: (1) suppressor T cell activity cannot be explained as simply being too much help; (2) feedback signals from target cells are of crucial importance in determining and maintaining the activity of suppressor T cells; (3) whenever T cells are triggered by antigen, suppression occurs. Immune responses only occur when countermanding signals are also generated. Both intrinsic and extrinsic adjuvanticity is the operational production of countermanding signals; (4) memory T cells are qualitatively different from normal T cells in their sensitivity to feedback signals and also in their susceptibility to suppression; (5) mature thymus dependent B cells cannot be rendered tolerant by the direct action of antigen, while immature and thymus independent B cells can; (6) the mechanism of suppression induced by exogenously administered antigens and that by normal differentiation products (i.e.: GVH; allotypes), is different; (7) generation of suppressor cells requires or results from complex interactions between subpopulations of cells, making it impossible under present conditions to determine which cell is doing what and to which; (8) further work is required before a full understanding of the importance, mechanism of action and other aspects of suppressor T cell function can be fully understood."}
{"id": "PMID:1101466", "title": "An evaluation of some factors affecting the detection of blood group antibodies by automated methods.", "content": "Some factors affecting the sensitivity of the automated methods for blood group antibody detection have been evaluated. The experiments revealed influencing differences between various albumin preparations. In the BMC method, one lot of albumin permitted no significant antibody detection. In the LISP technique, a plateau of maximum Polybrene activity was found. The beginning of this plateau depended on both the albumin preparation and the Polybrene lot. In the BMC method, methyl cellulose gave optimal sensitivity within a concentration range of 0.3 to 0.5 per cent. The stability of test cells stored in ACD at 4 C was studied. All test cells could be used safely up to two weeks. Cells from different donors showed variable reactivity after three weeks.", "contents": "An evaluation of some factors affecting the detection of blood group antibodies by automated methods. Some factors affecting the sensitivity of the automated methods for blood group antibody detection have been evaluated. The experiments revealed influencing differences between various albumin preparations. In the BMC method, one lot of albumin permitted no significant antibody detection. In the LISP technique, a plateau of maximum Polybrene activity was found. The beginning of this plateau depended on both the albumin preparation and the Polybrene lot. In the BMC method, methyl cellulose gave optimal sensitivity within a concentration range of 0.3 to 0.5 per cent. The stability of test cells stored in ACD at 4 C was studied. All test cells could be used safely up to two weeks. Cells from different donors showed variable reactivity after three weeks."}
{"id": "PMID:1101467", "title": "Comparison of HL-A typing with NIH trays and the NIH technique with typing by the fluorochromasia cytotoxicity assay.", "content": "A comparison of HL-A phenotypes determined with plates containing predispensed typing sera supplied by the NIH Serum Bank with phenotypes determined by the fluorochromasia cytotoxicity assay using local reagents showed that the NIH plates (as dispensed in 1972) had an insufficient number and variety of typing sera. Using NIH trays, it was difficult to detect HL-A9 and W19, both of which specificities are now known to have subtypes. The NIH trays also appeared to detect more HL-A antigens than were consistent with the current theory of two segregant series, each expressing a maximum of two antigens. The excess antigens could not be comparatively evaluated in view of the small number of sera defining each. With the NIH technique, it was impossible to interpret the reactions if the preparation had too many dead cells. A high proportion of dead cells was occasionally noted when lymphocytes had been previously frozen or obtained from lymph nodes. When the NIH and fluorochromasia techniques were compared using the same antisera and fresh lymphocytes, they proved equivalent in sensitivity.", "contents": "Comparison of HL-A typing with NIH trays and the NIH technique with typing by the fluorochromasia cytotoxicity assay. A comparison of HL-A phenotypes determined with plates containing predispensed typing sera supplied by the NIH Serum Bank with phenotypes determined by the fluorochromasia cytotoxicity assay using local reagents showed that the NIH plates (as dispensed in 1972) had an insufficient number and variety of typing sera. Using NIH trays, it was difficult to detect HL-A9 and W19, both of which specificities are now known to have subtypes. The NIH trays also appeared to detect more HL-A antigens than were consistent with the current theory of two segregant series, each expressing a maximum of two antigens. The excess antigens could not be comparatively evaluated in view of the small number of sera defining each. With the NIH technique, it was impossible to interpret the reactions if the preparation had too many dead cells. A high proportion of dead cells was occasionally noted when lymphocytes had been previously frozen or obtained from lymph nodes. When the NIH and fluorochromasia techniques were compared using the same antisera and fresh lymphocytes, they proved equivalent in sensitivity."}
{"id": "PMID:1101474", "title": "Skin grafting across H-Y incompatibility after organ culture.", "content": "Mouse skin maintained in organ culture for 7 days was transplanted across the H-Y histocompatibility barrier in two inbred strains: C57BL/6 and C3H/An. Cultured grafts were invariably rejected and at a faster rate than noncultured control grafts. Recipients of the cultured male skin grafts rejected subsequent noncultured male skin grafts in accelerated fashion.", "contents": "Skin grafting across H-Y incompatibility after organ culture. Mouse skin maintained in organ culture for 7 days was transplanted across the H-Y histocompatibility barrier in two inbred strains: C57BL/6 and C3H/An. Cultured grafts were invariably rejected and at a faster rate than noncultured control grafts. Recipients of the cultured male skin grafts rejected subsequent noncultured male skin grafts in accelerated fashion."}
{"id": "PMID:1101475", "title": "The effect of donor blood on renal allograft survival in DL-A tissue typed beagle littermates.", "content": "A single transfusion of 200 ml of donor blood 14 days before renal transplantation in prospectively DL-A tissue typed beagle littermates appeared to have an effect on graft survival. Seventeen per cent of the recipients did respond to the transfusion with formation of lymphocytotoxic and haemagglutinating antibodies. These \"responder dogs\" rejected kidney grafts in an accelerated way, compared with the \"nonresponders\" and with the nontreated control dogs. Responsiveness appeared to occur in pairs of littermates, which suggests that responding potency is genetically determined. There was histological evidence of acute arteritis in the renal grafts of responders, whereas cell-mediated rejection was noted in nonresponders.", "contents": "The effect of donor blood on renal allograft survival in DL-A tissue typed beagle littermates. A single transfusion of 200 ml of donor blood 14 days before renal transplantation in prospectively DL-A tissue typed beagle littermates appeared to have an effect on graft survival. Seventeen per cent of the recipients did respond to the transfusion with formation of lymphocytotoxic and haemagglutinating antibodies. These \"responder dogs\" rejected kidney grafts in an accelerated way, compared with the \"nonresponders\" and with the nontreated control dogs. Responsiveness appeared to occur in pairs of littermates, which suggests that responding potency is genetically determined. There was histological evidence of acute arteritis in the renal grafts of responders, whereas cell-mediated rejection was noted in nonresponders."}
{"id": "PMID:1101476", "title": "Inhibition of tumor growth in syngeneic chimeric mice mediated by a depletion of suppressor T cells.", "content": "Syngeneic chimeric (lethally irradiated and reconstituted with syngeneic bone marrow cells) mice manifested an increased resistance to the development of Lewis lung carcinoma. In addition, these mice had a higher response to polyvinylpyrrolidone and a reduced reactivity to T mitogens. The present findings suggest that syngeneic chimeric mice lack suppressor T cells shown to regulate the development of Lewis lung tumor and the response to polyvinylpyrrolidone. Other components of the T cell population, such as helper cells responding to sheep red blood cells or cells involved in allograft rejection, assayed in these syngeneic chimeras were found unaffected. The fact that chimeric mice are deficient in a certain suppressor T cell population whereas other T activities are normal suggests the existence of different cell lines within the T cell population.", "contents": "Inhibition of tumor growth in syngeneic chimeric mice mediated by a depletion of suppressor T cells. Syngeneic chimeric (lethally irradiated and reconstituted with syngeneic bone marrow cells) mice manifested an increased resistance to the development of Lewis lung carcinoma. In addition, these mice had a higher response to polyvinylpyrrolidone and a reduced reactivity to T mitogens. The present findings suggest that syngeneic chimeric mice lack suppressor T cells shown to regulate the development of Lewis lung tumor and the response to polyvinylpyrrolidone. Other components of the T cell population, such as helper cells responding to sheep red blood cells or cells involved in allograft rejection, assayed in these syngeneic chimeras were found unaffected. The fact that chimeric mice are deficient in a certain suppressor T cell population whereas other T activities are normal suggests the existence of different cell lines within the T cell population."}
{"id": "PMID:1101478", "title": "Effect of immunosuppressive therapy for renal allografts on the number of circulating sheep red blood cells rosetting cells.", "content": "Patients receiving renal allografts from relatively incompatible donors were randomly assigned to one of two immunosuppressive regimens: azathioprine and prednisone with or without a 2-week course of horse antihuman thymocyte globulin (ATG). The number of circulating lymphocytes fell to about one-third of pretreatment values in both groups of patients. In patients given ATG, the proportion of sheep red blood cell-rosetting lymphocytes (SRBC-R) fell promptly to less than 10% of pretreatment values. In contrast, the percentage of SRBC-R remained at about 70% in patients receiving only prednisone and azathioprine. The addition of ATG reduced the number of SRBC-R/mm3 to one-tenth to one-thirtieth the number seen in non-ATG-treated patients. Plasma of patients undergoing therapy did not inhibit rosetting in vitro. It is proposed that the monitoring of circulating rosetting cells may be a useful clinical guide to the degree of T cell immunosuppression. It is also suggested that the regimen of azathioprine, prednisone, and ATG results in a more effective suppression of circulating T cells than that produced by azathioprine and prednisone alone.", "contents": "Effect of immunosuppressive therapy for renal allografts on the number of circulating sheep red blood cells rosetting cells. Patients receiving renal allografts from relatively incompatible donors were randomly assigned to one of two immunosuppressive regimens: azathioprine and prednisone with or without a 2-week course of horse antihuman thymocyte globulin (ATG). The number of circulating lymphocytes fell to about one-third of pretreatment values in both groups of patients. In patients given ATG, the proportion of sheep red blood cell-rosetting lymphocytes (SRBC-R) fell promptly to less than 10% of pretreatment values. In contrast, the percentage of SRBC-R remained at about 70% in patients receiving only prednisone and azathioprine. The addition of ATG reduced the number of SRBC-R/mm3 to one-tenth to one-thirtieth the number seen in non-ATG-treated patients. Plasma of patients undergoing therapy did not inhibit rosetting in vitro. It is proposed that the monitoring of circulating rosetting cells may be a useful clinical guide to the degree of T cell immunosuppression. It is also suggested that the regimen of azathioprine, prednisone, and ATG results in a more effective suppression of circulating T cells than that produced by azathioprine and prednisone alone."}
{"id": "PMID:1101479", "title": "Sex-determined antigens in rats detected by bone marrow transplantation but not skin grafting.", "content": "Skin grafting in untreated rats and bone marrow transplantation into minimally immunosuppressed recipients have been used to investigate histocompatibility differences associated with the Y chromosome. Male skin survived for upwards of 120 days with no sign of rejection when grafted into female rats in circumstances where male bone marrow was rejected. Y chromosome-associated antigens were only detected with skin grafting by prior exposure of the female rats to male cells. It is concluded that bone marrow grafting into minimally immunosuppressed recipients may provide a more sensitive index of minor histocompatibility differences than skin grafting. The need to recognise donor cells in recipients prepared for bone marrow grafting with cytotoxic chemicals is emphasized.", "contents": "Sex-determined antigens in rats detected by bone marrow transplantation but not skin grafting. Skin grafting in untreated rats and bone marrow transplantation into minimally immunosuppressed recipients have been used to investigate histocompatibility differences associated with the Y chromosome. Male skin survived for upwards of 120 days with no sign of rejection when grafted into female rats in circumstances where male bone marrow was rejected. Y chromosome-associated antigens were only detected with skin grafting by prior exposure of the female rats to male cells. It is concluded that bone marrow grafting into minimally immunosuppressed recipients may provide a more sensitive index of minor histocompatibility differences than skin grafting. The need to recognise donor cells in recipients prepared for bone marrow grafting with cytotoxic chemicals is emphasized."}
{"id": "PMID:1101480", "title": "Xenogeneic skin and kidney transplants in a closely related canine system, fox-dog.", "content": "Fox kidney and skin grafts were transplanted into dog recipients. Fox kidneys, transplanted en bloc into untreated dogs, survived 6.2 +/- 0.4 days. The skin transplants survived 5.9 +/- 1.4 days. The grafted kidneys showed almost normal function before rejection. Both skin and kidney rejection were mediated through a cellular mechansim. Performed natural antibodies against donor tissue were not present in the serum of the recipients. These results combined with absorption studies suggested a close relationship between fox and dog, but different number and morphology of chromosomes, immunoelectrophoretic patterns of serum proteins, and disparities of the transplantation antigens proved that the fox is a species quite separate from the dog. It was concluded that the fox-dog system, with its similarity to the chimpanzeeman relationship, offers a unique model to study clinically applicable methods of managing xenografts between closely related species.", "contents": "Xenogeneic skin and kidney transplants in a closely related canine system, fox-dog. Fox kidney and skin grafts were transplanted into dog recipients. Fox kidneys, transplanted en bloc into untreated dogs, survived 6.2 +/- 0.4 days. The skin transplants survived 5.9 +/- 1.4 days. The grafted kidneys showed almost normal function before rejection. Both skin and kidney rejection were mediated through a cellular mechansim. Performed natural antibodies against donor tissue were not present in the serum of the recipients. These results combined with absorption studies suggested a close relationship between fox and dog, but different number and morphology of chromosomes, immunoelectrophoretic patterns of serum proteins, and disparities of the transplantation antigens proved that the fox is a species quite separate from the dog. It was concluded that the fox-dog system, with its similarity to the chimpanzeeman relationship, offers a unique model to study clinically applicable methods of managing xenografts between closely related species."}
{"id": "PMID:1101486", "title": "Attempts to compare the effectiveness of blocking factors and enhancing antibodies in vivo and in vitro.", "content": "Sera from rats carrying tolerated skin allografts were tested for the presence of blocking activity in vitro. Sera with blocking activity had no effect on transplantation tolerance induction in newborn animals. Immunological enhancement of tumor growth was procured by passive transfer of serum from tolerant animals bearing skin allografts. It made no difference whether or not the serum contained blocking activity in vitro. These results suggest that there is no relationship between blocking factors and enhancing activity in vivo.", "contents": "Attempts to compare the effectiveness of blocking factors and enhancing antibodies in vivo and in vitro. Sera from rats carrying tolerated skin allografts were tested for the presence of blocking activity in vitro. Sera with blocking activity had no effect on transplantation tolerance induction in newborn animals. Immunological enhancement of tumor growth was procured by passive transfer of serum from tolerant animals bearing skin allografts. It made no difference whether or not the serum contained blocking activity in vitro. These results suggest that there is no relationship between blocking factors and enhancing activity in vivo."}
{"id": "PMID:1101487", "title": "Longitudinal malaria studies in rural Northeast Thailand. Chloroquine treatment of falciparum malaria infections.", "content": "The efficacy of chloroquine in the treatment of falciparum malaria was studied in two villages in Northeast Thailand, an area endemic for chloroquine-resistant falciparum infections. Chloroquine did not appear to reduce the duration or density of parasitemias experienced by asymptomatic villagers, but did benefit, usually temporarily, many subjects with symptomatic or high-density infections. These observations suggest that the high prevalence of chloroquine-resistant infections in the villages is similar to data from the hospital and clinics serving the area. The question whether chloroquine should remain available to this population should be evaluated.", "contents": "Longitudinal malaria studies in rural Northeast Thailand. Chloroquine treatment of falciparum malaria infections. The efficacy of chloroquine in the treatment of falciparum malaria was studied in two villages in Northeast Thailand, an area endemic for chloroquine-resistant falciparum infections. Chloroquine did not appear to reduce the duration or density of parasitemias experienced by asymptomatic villagers, but did benefit, usually temporarily, many subjects with symptomatic or high-density infections. These observations suggest that the high prevalence of chloroquine-resistant infections in the villages is similar to data from the hospital and clinics serving the area. The question whether chloroquine should remain available to this population should be evaluated."}
{"id": "PMID:1101489", "title": "[Ultrastructure of the secretory cells of the pancreatic islets in human fetuses].", "content": "An electron microscopic study of pancreatic islets of human fetuses 16--28 weeks old discovered four types of secretory cells -- B, A, D and type IV, which has not yet been given a generally accepted name. These cells contain small secretory granules more dense than granules D. In three cases endocrine elements with \"mixed\" granulation (D--A) were noted. The formation of insulin- and glucagon-containing granules in the ergastoplasm and Golgi complex was observed. The hormone secretion is realized by emyocitosis.", "contents": "[Ultrastructure of the secretory cells of the pancreatic islets in human fetuses]. An electron microscopic study of pancreatic islets of human fetuses 16--28 weeks old discovered four types of secretory cells -- B, A, D and type IV, which has not yet been given a generally accepted name. These cells contain small secretory granules more dense than granules D. In three cases endocrine elements with \"mixed\" granulation (D--A) were noted. The formation of insulin- and glucagon-containing granules in the ergastoplasm and Golgi complex was observed. The hormone secretion is realized by emyocitosis."}
{"id": "PMID:1101490", "title": "[Comparative morphometric study of the ultrastructure of cells in vivo and in vitro].", "content": "A morphometrical study of the fine structure of normal and malignant cells, both taken immediately from the organism (in vivo) or previously cultured (in vitro) was carried out. A high level on the mitochondrial morphological coefficient -- Km was discovered in normal and malignant cells in vitro. Unlike, the ergastoplasmic morphological coefficient -- Ke was high in the in vivo cells and low in the in vitro cells. The in vitro factor causes considerable changes in the fine structure of cells, which are more important than distinctions between normal and malignant cells. This fact must be taken into account in morphological studies of cells in vitro.", "contents": "[Comparative morphometric study of the ultrastructure of cells in vivo and in vitro]. A morphometrical study of the fine structure of normal and malignant cells, both taken immediately from the organism (in vivo) or previously cultured (in vitro) was carried out. A high level on the mitochondrial morphological coefficient -- Km was discovered in normal and malignant cells in vitro. Unlike, the ergastoplasmic morphological coefficient -- Ke was high in the in vivo cells and low in the in vitro cells. The in vitro factor causes considerable changes in the fine structure of cells, which are more important than distinctions between normal and malignant cells. This fact must be taken into account in morphological studies of cells in vitro."}
{"id": "PMID:1101491", "title": "[Rapid method of obtaining ultrathin sections in monolayer embedding].", "content": "It is proposed to employ a simple optical method for a correct relative adjustment of the knife and the roof of the piramid for obtaining ultrathin sections of monolayer embedding. For the material embedding, plastic cuvets and a lavsan film are employed, this technique facilitating the process of embedding.", "contents": "[Rapid method of obtaining ultrathin sections in monolayer embedding]. It is proposed to employ a simple optical method for a correct relative adjustment of the knife and the roof of the piramid for obtaining ultrathin sections of monolayer embedding. For the material embedding, plastic cuvets and a lavsan film are employed, this technique facilitating the process of embedding."}
{"id": "PMID:1101500", "title": "Quantitative colour-ultrasonography by means of a computer aided simultaneous tomogram.", "content": "In this newly developed method, the ultrasonic diagnosis has become quantitative. The data will remain quantitative so far as an image storage tube is used. With the conventional storage type CRT, it is impossible to memorize the reflected echo signals of different strength levels. But with our method, it becomes possible to memorize the images according  to individual levels. By the direct digitalization of the ultrasonic echo images, more information is obtained from the reflected echo signals. By processing the original information with a computer, more accurate diagnosis is possible. It is hoped and believed, that this computer aided ultrasonic examination, with single scan display in colour or black and white, will further promote ultrasonic diagnosis in future.", "contents": "Quantitative colour-ultrasonography by means of a computer aided simultaneous tomogram. In this newly developed method, the ultrasonic diagnosis has become quantitative. The data will remain quantitative so far as an image storage tube is used. With the conventional storage type CRT, it is impossible to memorize the reflected echo signals of different strength levels. But with our method, it becomes possible to memorize the images according  to individual levels. By the direct digitalization of the ultrasonic echo images, more information is obtained from the reflected echo signals. By processing the original information with a computer, more accurate diagnosis is possible. It is hoped and believed, that this computer aided ultrasonic examination, with single scan display in colour or black and white, will further promote ultrasonic diagnosis in future."}
{"id": "PMID:1101505", "title": "Rotational skin flaps to cover wound defect in groin.", "content": "A technique of a lower relaxing incision and use of a rotational skin flap from the anterolateral thigh to close an extensive defect in the inguinal area is described. Its use is especially applicable for palliation in patients with large fungating metastases in the inguinal region.", "contents": "Rotational skin flaps to cover wound defect in groin. A technique of a lower relaxing incision and use of a rotational skin flap from the anterolateral thigh to close an extensive defect in the inguinal area is described. Its use is especially applicable for palliation in patients with large fungating metastases in the inguinal region."}
{"id": "PMID:1101519", "title": "[Phage typing of strains of S. enteritidis isolated in Bulgaria].", "content": "Studied were a total of 221 strains of Salmonella enteritidis isolated from birds, pigs, cattle, dogs, rodents, forage, environment, food products of animal origin, and human beings, in terms of their phage typing, in order to study the epizootiology of the foci of infection. Twelve phages were established: 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, 15, 19, 20 and 21. Most widely distributed proved phage type 6a, followed by phage type 2a. The remaining phages occured more rarely, and some of them--15, 19, 20 and 21--were found only in particular districts and farms.", "contents": "[Phage typing of strains of S. enteritidis isolated in Bulgaria]. Studied were a total of 221 strains of Salmonella enteritidis isolated from birds, pigs, cattle, dogs, rodents, forage, environment, food products of animal origin, and human beings, in terms of their phage typing, in order to study the epizootiology of the foci of infection. Twelve phages were established: 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, 15, 19, 20 and 21. Most widely distributed proved phage type 6a, followed by phage type 2a. The remaining phages occured more rarely, and some of them--15, 19, 20 and 21--were found only in particular districts and farms."}
{"id": "PMID:1101537", "title": "[Permeability of the mitochondrial membranes of the organs of white rats innoculated with Walker carcinoma to lipoic acid and thiamine labelled with S35].", "content": "Mitochondria isolated from organs of intact white rats and those with Walker carcinoma were incubated in Gubler medium containing S35-lipoic acid or S35-thiamine. An accumulation of lipoic acid by mitochondria is higher in tumor-bearing animals, while that of thiamine in most organs is lowered. In healthy animals mitochondria were found to accumulate S35-thiamine more intensively than S35-lipoic acid. In tumor animals these indices become nearly similar, while organ differences are leveled.", "contents": "[Permeability of the mitochondrial membranes of the organs of white rats innoculated with Walker carcinoma to lipoic acid and thiamine labelled with S35]. Mitochondria isolated from organs of intact white rats and those with Walker carcinoma were incubated in Gubler medium containing S35-lipoic acid or S35-thiamine. An accumulation of lipoic acid by mitochondria is higher in tumor-bearing animals, while that of thiamine in most organs is lowered. In healthy animals mitochondria were found to accumulate S35-thiamine more intensively than S35-lipoic acid. In tumor animals these indices become nearly similar, while organ differences are leveled."}
{"id": "PMID:1101538", "title": "[Prevention and treatment of cancer of the cervical stump].", "content": "Of total number of patients with cervical cancer (12687) since 1962 to 1972 155 (1.2%) were subjected to therapy for cancer of the cervical stump. In 5 of 155 patients (3.2%) preinvasive cancer of the cervical stump was observed. Preliminary supravaginal hysterectomy was performed for benign pathological processes of the female genitalia, hysterectomy was performed for benign pathological processes of the female genitalia, including 86.5%--for uterine fibromyoma. Recently, a prolonged interval (10 years and longer) elapsed since supravaginal hysterectomy is typical for patients in whom cancer of the cervical stump develop. Hence, a careful follow-up of patients during the life period after supravaginal hysterectomy seems to be hardly feasible as it should be recommended, and it could not be an adequate prophylactic measure without using a cytologic assay. An idea of the rationality of using extirpation in climacteric and menopausal patients with fibromyoma is being substantiated.", "contents": "[Prevention and treatment of cancer of the cervical stump]. Of total number of patients with cervical cancer (12687) since 1962 to 1972 155 (1.2%) were subjected to therapy for cancer of the cervical stump. In 5 of 155 patients (3.2%) preinvasive cancer of the cervical stump was observed. Preliminary supravaginal hysterectomy was performed for benign pathological processes of the female genitalia, hysterectomy was performed for benign pathological processes of the female genitalia, including 86.5%--for uterine fibromyoma. Recently, a prolonged interval (10 years and longer) elapsed since supravaginal hysterectomy is typical for patients in whom cancer of the cervical stump develop. Hence, a careful follow-up of patients during the life period after supravaginal hysterectomy seems to be hardly feasible as it should be recommended, and it could not be an adequate prophylactic measure without using a cytologic assay. An idea of the rationality of using extirpation in climacteric and menopausal patients with fibromyoma is being substantiated."}
{"id": "PMID:1101541", "title": "A mild method for the isolation of folded chromosomes from Escherichia coli.", "content": "Until now it has not been possible to obtain nuclear bodies from Escherichia coli after treatment with rifampicin. It was generally assumed that the cross-connections between the DNA double strands which are sensitive towards ribonuclease are destroyed under the influence of inhibitors of RNA synthesis like rifampicin. In this paper a new lysis procedure is described for preparing nuclear bodies from E. coli. These particles differ in some respects, especially in salt sensitivity from those prepared by earlier methods. Using the new lysis method it is also possible to obtain folded chromosomes from cells after treatment with rifampicin. These nuclear bodies can be destroyed by ribonuclease. Therefore, it has to be postulated that a fraction of RNA being sufficient to hold the chromosome in the folded shape is not susceptible to the action of rifampicin.", "contents": "A mild method for the isolation of folded chromosomes from Escherichia coli. Until now it has not been possible to obtain nuclear bodies from Escherichia coli after treatment with rifampicin. It was generally assumed that the cross-connections between the DNA double strands which are sensitive towards ribonuclease are destroyed under the influence of inhibitors of RNA synthesis like rifampicin. In this paper a new lysis procedure is described for preparing nuclear bodies from E. coli. These particles differ in some respects, especially in salt sensitivity from those prepared by earlier methods. Using the new lysis method it is also possible to obtain folded chromosomes from cells after treatment with rifampicin. These nuclear bodies can be destroyed by ribonuclease. Therefore, it has to be postulated that a fraction of RNA being sufficient to hold the chromosome in the folded shape is not susceptible to the action of rifampicin."}
{"id": "PMID:1101542", "title": "Unfolding of the chromosome of Escherichia coli after treatment with rifampicin.", "content": "Until recently it has been assumed that the factors being responsible for the condensation of the DNA in the nucleoid of Escherichia coli are destroyed by rifampicin because it has been impossible to obtain folded chromosomes from cells treated with this inhibitor. In this paper it is shown by viscosity and sedimentation measurements that unfolding of the DNA does not take place during the process of the cell lysis as it should be predicted from this assumption, but is occurring distinctly afterwards. Since the observed unfolding process is too slow to be caused simply by molecular movements it is concluded that it is brought about by the action of salts or detergents of the lysis medium. The structure of the nucleoid is still intact in vivo despite inhibition of RNA synthesis by rifampicin.", "contents": "Unfolding of the chromosome of Escherichia coli after treatment with rifampicin. Until recently it has been assumed that the factors being responsible for the condensation of the DNA in the nucleoid of Escherichia coli are destroyed by rifampicin because it has been impossible to obtain folded chromosomes from cells treated with this inhibitor. In this paper it is shown by viscosity and sedimentation measurements that unfolding of the DNA does not take place during the process of the cell lysis as it should be predicted from this assumption, but is occurring distinctly afterwards. Since the observed unfolding process is too slow to be caused simply by molecular movements it is concluded that it is brought about by the action of salts or detergents of the lysis medium. The structure of the nucleoid is still intact in vivo despite inhibition of RNA synthesis by rifampicin."}
{"id": "PMID:1101546", "title": "Rat liver alcohol dehydrogenase isozymes: influence of infection with Trypanosoma.", "content": "A study has been made of changes in alcohol dehydrogenase (ADH) isozymes in livers of rats infected with the following trypanosomes: Trypanosoma cruzi, T. rhodesiense, T. congolense, and six geographical isolates of T. lewisi. A control group of the same age was injected with saline, and another left uninjected. Liver homogenates were electrophoresed in noble agar gels followed by formazan staining according to the method of Ursprung and Leone (1965). Of a total of 25 replicates, 15 showed that the level of rat liver ADH isozymes was similar in the uninjected and saline injected animals; 6 replicates showed a slightly higher level of ADH isozymes in uninjected than saline injected control rat livers, and 4 replicates had a higher level in saline injected than in the uninjected. With either 1-octanol or ethanol as substrate, a marked elevation of ADH activity was observed beginning with day 7 post inoculation in rat livers infected with T. cruzi, T. lewisi, and T. congolense, or as early as day 4 post inoculation with T. rhodesiense. The elevation persisted 59 days or more in rat livers infected with T. lewisi, but rats infected with the pathogenic species usually died 5 to 14 days after infection. The elevation of ADH isozymes has also been demonstrated spectrophotometrically for the first 5 minutes of reaction time, using acetaldehyde as substrate. Also observed in livers infected with pathogenic species and certain strains of T. lewisi were: (1) ADH isozyme pattern changes involving an elevated level of certain isozymes only, and (2) abnormal isozyme mobilities.", "contents": "Rat liver alcohol dehydrogenase isozymes: influence of infection with Trypanosoma. A study has been made of changes in alcohol dehydrogenase (ADH) isozymes in livers of rats infected with the following trypanosomes: Trypanosoma cruzi, T. rhodesiense, T. congolense, and six geographical isolates of T. lewisi. A control group of the same age was injected with saline, and another left uninjected. Liver homogenates were electrophoresed in noble agar gels followed by formazan staining according to the method of Ursprung and Leone (1965). Of a total of 25 replicates, 15 showed that the level of rat liver ADH isozymes was similar in the uninjected and saline injected animals; 6 replicates showed a slightly higher level of ADH isozymes in uninjected than saline injected control rat livers, and 4 replicates had a higher level in saline injected than in the uninjected. With either 1-octanol or ethanol as substrate, a marked elevation of ADH activity was observed beginning with day 7 post inoculation in rat livers infected with T. cruzi, T. lewisi, and T. congolense, or as early as day 4 post inoculation with T. rhodesiense. The elevation persisted 59 days or more in rat livers infected with T. lewisi, but rats infected with the pathogenic species usually died 5 to 14 days after infection. The elevation of ADH isozymes has also been demonstrated spectrophotometrically for the first 5 minutes of reaction time, using acetaldehyde as substrate. Also observed in livers infected with pathogenic species and certain strains of T. lewisi were: (1) ADH isozyme pattern changes involving an elevated level of certain isozymes only, and (2) abnormal isozyme mobilities."}
{"id": "PMID:1101548", "title": "[Correction osteotomy of tibia for malalignment at the knee and the bone-suture (author's transl)].", "content": "Correction osteotomy of the tibia in genu valgum and varum is discussed on the basis of the literature and two cases. After considering critically the well-known techniques of fixation, the almost forgotten bone-suture with silk or Perlon is recalled which may replace osteosynthesis. The advantages of the plaster cast of the leg which permits much earlier weightbearing then osteosynthesis and adds to bony union are emphasized. The author warns against too early mobilization.", "contents": "[Correction osteotomy of tibia for malalignment at the knee and the bone-suture (author's transl)]. Correction osteotomy of the tibia in genu valgum and varum is discussed on the basis of the literature and two cases. After considering critically the well-known techniques of fixation, the almost forgotten bone-suture with silk or Perlon is recalled which may replace osteosynthesis. The advantages of the plaster cast of the leg which permits much earlier weightbearing then osteosynthesis and adds to bony union are emphasized. The author warns against too early mobilization."}
{"id": "PMID:1101573", "title": "[Serogrouping of Escherichia coli from urinary tract infections (author's transl)].", "content": "Serological grouping with 44 anti-O-sera was performed on 215 E. coli strains isolated from urine of patients with significant bacteriuria. 95 strains (44.2%) were typable, 34 strains (15.8%) proved to be rough strains, and 86 strains (40%) were untypbale. The O-groups 2, 6, 9 and 75 were found to be more frequent in patients with chronic pyelonephritis. Among pregnant women with asymptomatic bacteriuria a typical frequency was not observed.", "contents": "[Serogrouping of Escherichia coli from urinary tract infections (author's transl)]. Serological grouping with 44 anti-O-sera was performed on 215 E. coli strains isolated from urine of patients with significant bacteriuria. 95 strains (44.2%) were typable, 34 strains (15.8%) proved to be rough strains, and 86 strains (40%) were untypbale. The O-groups 2, 6, 9 and 75 were found to be more frequent in patients with chronic pyelonephritis. Among pregnant women with asymptomatic bacteriuria a typical frequency was not observed."}
{"id": "PMID:1101574", "title": "Profuse fimbriae conferring O-inagglutinability to several strains of S. typhi-murium and S. enteritidis isolated from pasta products. Cultural, morphological, and serological experiments.", "content": "During the last 2 years several O-inagglutinable Salmonella strains were isolated from pasta products the typing of which presented great difficulties. Serological and morphological tests (by electron microscope) revealed a fimbrial antigen which was thermolabile to a certain degree. This antigen represents (in corroboration of J.P. Dugiud) a special type of fimbriae of Salmonella and is morphologically different from type 1 and typ3-2 fimbriae previously described. It is probably an abnormal mutant form of fimbriae. Morphologically characteristic are its very much finer structure of filaments (2,7 nm) (Type-1 and type-2 of fimbriae measure in the range 6-8 nm.) and the 2,7 nm) (Type-1 and type-2 of fimbriae measure in the range 6-8 nm.). and the 2,7 nm) (Tpe-1 and type-2 aof fimbriae measure in the range 6-8 nm.) and the 2,7 nm) (Tpe-1 and type-2 of fimbriae measure in the range 6-8 nm.) and the large number of filamentous appendages...", "contents": "Profuse fimbriae conferring O-inagglutinability to several strains of S. typhi-murium and S. enteritidis isolated from pasta products. Cultural, morphological, and serological experiments. During the last 2 years several O-inagglutinable Salmonella strains were isolated from pasta products the typing of which presented great difficulties. Serological and morphological tests (by electron microscope) revealed a fimbrial antigen which was thermolabile to a certain degree. This antigen represents (in corroboration of J.P. Dugiud) a special type of fimbriae of Salmonella and is morphologically different from type 1 and typ3-2 fimbriae previously described. It is probably an abnormal mutant form of fimbriae. Morphologically characteristic are its very much finer structure of filaments (2,7 nm) (Type-1 and type-2 of fimbriae measure in the range 6-8 nm.) and the 2,7 nm) (Type-1 and type-2 of fimbriae measure in the range 6-8 nm.). and the 2,7 nm) (Tpe-1 and type-2 aof fimbriae measure in the range 6-8 nm.) and the 2,7 nm) (Tpe-1 and type-2 of fimbriae measure in the range 6-8 nm.) and the large number of filamentous appendages..."}
{"id": "PMID:1101575", "title": "[Contributions to the strain-specific virulence of Cryptococcus neoformans. Animal experiments with two C. neoformans-strains isolated from bird manure. Preliminary report (author's transl)].", "content": "The results briefly presented here highlight some of the observations met with during the course of a study aimed at finding out differential pathogenic behavior of the two strains of Cryptococcus neoformans i.e. W71/A117 and W2/A94 with special reference to cryptococcoma formation. Both strains were isolated from bird excreta but differed in their gross and microscopic morphology. Groups of male albino mice NMRI were separately inoculated, intramuscularly, with a comparable dose of the two strains. All the 50 animals challenged with W71/A117 developed macroscopically distinct cryptococcoma of variable size, and fatally progressive disease, involving most of the internal organs, namely, brain, heart, lung, liver, spleen and kidneys. On the contrary, only 34 of the 50 mice infected with the strain W2/A94 showed cryptococcoma formation at the site of inoculation, which were comparatively much smaller in size and gradually diminished. None of the animals, observed over a period of 93 days, died, and showed any sign of metastasis. In another series of experiment, only one instance of mortality was observed in a group of 80 mice inoculated intraperitoneally with about 2 X 10(7) viable cells of this strain. However, the fungus could be recovered, in a majority of cases, only from the brain of animals sacrificed after one month, though most of them showed no sign of sickness. The number of mice yielding positive cultures gradually decreased, and after 87 days the fungus could not be isolated from any organ. The high morbidity and mortality in mice caused by strains W71/A117 was significantly lowered when the animals were infected intramuscularly 3 months ago with the strain W2/A94. After an observation period of 61 days, 91% of the double infected animals were still alive in comparison to 23% survival among the animals challenged with the strain W71/A117 only.", "contents": "[Contributions to the strain-specific virulence of Cryptococcus neoformans. Animal experiments with two C. neoformans-strains isolated from bird manure. Preliminary report (author's transl)]. The results briefly presented here highlight some of the observations met with during the course of a study aimed at finding out differential pathogenic behavior of the two strains of Cryptococcus neoformans i.e. W71/A117 and W2/A94 with special reference to cryptococcoma formation. Both strains were isolated from bird excreta but differed in their gross and microscopic morphology. Groups of male albino mice NMRI were separately inoculated, intramuscularly, with a comparable dose of the two strains. All the 50 animals challenged with W71/A117 developed macroscopically distinct cryptococcoma of variable size, and fatally progressive disease, involving most of the internal organs, namely, brain, heart, lung, liver, spleen and kidneys. On the contrary, only 34 of the 50 mice infected with the strain W2/A94 showed cryptococcoma formation at the site of inoculation, which were comparatively much smaller in size and gradually diminished. None of the animals, observed over a period of 93 days, died, and showed any sign of metastasis. In another series of experiment, only one instance of mortality was observed in a group of 80 mice inoculated intraperitoneally with about 2 X 10(7) viable cells of this strain. However, the fungus could be recovered, in a majority of cases, only from the brain of animals sacrificed after one month, though most of them showed no sign of sickness. The number of mice yielding positive cultures gradually decreased, and after 87 days the fungus could not be isolated from any organ. The high morbidity and mortality in mice caused by strains W71/A117 was significantly lowered when the animals were infected intramuscularly 3 months ago with the strain W2/A94. After an observation period of 61 days, 91% of the double infected animals were still alive in comparison to 23% survival among the animals challenged with the strain W71/A117 only."}
{"id": "PMID:1101576", "title": "[Studies on the relationship of temperent phages and bacteriocines of streptococcus faecium (author's transl)].", "content": "Two bacteriocins (enterocin E1A and E1B) as well as a complete bacteriophage (PE1) were produced by Streptococcus faecium strain E1. Although the phage could be demonstrated by electron microscopy it was not possible to observe phages or phage-like particles in the purified preparation of the large enterocin E1B. Phage PE1 had a much smaller activity spectrum than that of enterocin E1A and E1B, inhibiting only one strain of Streptococcus faecium and one strain of Streptococcus salivarius. The enterocins were not neutralized by antiphage sera, thereby suggesting that the enterocins and the phage are chemically unrelated.", "contents": "[Studies on the relationship of temperent phages and bacteriocines of streptococcus faecium (author's transl)]. Two bacteriocins (enterocin E1A and E1B) as well as a complete bacteriophage (PE1) were produced by Streptococcus faecium strain E1. Although the phage could be demonstrated by electron microscopy it was not possible to observe phages or phage-like particles in the purified preparation of the large enterocin E1B. Phage PE1 had a much smaller activity spectrum than that of enterocin E1A and E1B, inhibiting only one strain of Streptococcus faecium and one strain of Streptococcus salivarius. The enterocins were not neutralized by antiphage sera, thereby suggesting that the enterocins and the phage are chemically unrelated."}
{"id": "PMID:1101577", "title": "[The endotoxin from S-, R- and M-forms of enteropathogenic E. coli O 149: pyrogenicity, Shwartzman-phenomenon and hypersensitivity (author's transl)].", "content": "The authors are interested in elucidation of the role of endotoxin from S, R- and M-forms of E. coli O 149, and of the role of hypersensitivity in pathogenesis of Colibacillosis. It was shown in this experiment that all S, R- and M-form of E. coli O149 possess endotoxin with almost the same pyrogenicity in normal rabbits. The pyrogenicity increases enormously in the hypersensitized rabbit. There were also not observed differences between endotoxins extracted from S, R, M-mutnants. All 3 endotoxins evoke in normal rabbits the skin Shwartzman-Phenomenon, the S-endotoxin was there much stronger. This phenomenon increases in capacity in hypersensitized rabbits. Thus again, the role of hypersensitivity in Colibacillosis was proved to be important.", "contents": "[The endotoxin from S-, R- and M-forms of enteropathogenic E. coli O 149: pyrogenicity, Shwartzman-phenomenon and hypersensitivity (author's transl)]. The authors are interested in elucidation of the role of endotoxin from S, R- and M-forms of E. coli O 149, and of the role of hypersensitivity in pathogenesis of Colibacillosis. It was shown in this experiment that all S, R- and M-form of E. coli O149 possess endotoxin with almost the same pyrogenicity in normal rabbits. The pyrogenicity increases enormously in the hypersensitized rabbit. There were also not observed differences between endotoxins extracted from S, R, M-mutnants. All 3 endotoxins evoke in normal rabbits the skin Shwartzman-Phenomenon, the S-endotoxin was there much stronger. This phenomenon increases in capacity in hypersensitized rabbits. Thus again, the role of hypersensitivity in Colibacillosis was proved to be important."}
{"id": "PMID:1101571", "title": "[Do the severity and duration of rheumatoid arthritis influence pulmonary ventilation?].", "content": "Pulmonary ventilation was studied in 100 patients with rheumatoid arthritis of an outpatient clinic, using whole-body plethysmography, spirography, and pneumometry. The patients with more or less severe joint involvement, as well as the patients with shorter or longer duration of their disease, did not show any significant difference in their dynamic and static pulmonary fuction. Patients with serious joint destructions showed a bronchial obstruction in 29%. 36 patients who were treated with thioglucose gold had a significantly better expiration capacity. A causal connection with the underlying disease is discussed. Further examinations should take therapeutic procedures and longitudinal studies into consideration. It is doubtful that significant restriction of ventilation is present in rheumatoid-arthritis. Methodically there was a good argreement between whole body plethysmography and pneumometry.", "contents": "[Do the severity and duration of rheumatoid arthritis influence pulmonary ventilation?]. Pulmonary ventilation was studied in 100 patients with rheumatoid arthritis of an outpatient clinic, using whole-body plethysmography, spirography, and pneumometry. The patients with more or less severe joint involvement, as well as the patients with shorter or longer duration of their disease, did not show any significant difference in their dynamic and static pulmonary fuction. Patients with serious joint destructions showed a bronchial obstruction in 29%. 36 patients who were treated with thioglucose gold had a significantly better expiration capacity. A causal connection with the underlying disease is discussed. Further examinations should take therapeutic procedures and longitudinal studies into consideration. It is doubtful that significant restriction of ventilation is present in rheumatoid-arthritis. Methodically there was a good argreement between whole body plethysmography and pneumometry."}
{"id": "PMID:1101578", "title": "[Mucous (M) mutants of enteropathogenic, septicemic and saprophytic E. coli (author's transl)].", "content": "Mucoid M mutants of enteropathogenic (for suckling pigs), septicemic (for calves), and saprophytic E. Coli were subject of the study. The characteristics of the M colonies have been listed in Table 1. Both spontaneously occurring M mutants which had been isolated by culturing of E. coli strains (S form) in normal horse serum (undiluted) for several weeks were observed. Enzymatic-biochemical tests revealed the existence of M mutants with full enzmatic activity which are identical with the homologous S and R forms. There were also M mutants exhibiting a considerably reduced enzymatic activity. Antigenic-serological analysis (agglutination) exhibited M mutants which were agglutinated by the homologous sera, at low and high titres. In contrast to this, one group of M mutants was found not to be agglutinable, despite autoclaving at 120 degrees C for 2 hours. Some mutants cultured in broth for several weeks were re-exhibiting S colonies of full biochemical activity and full antigen reactivity against homologous agglutinating sera.", "contents": "[Mucous (M) mutants of enteropathogenic, septicemic and saprophytic E. coli (author's transl)]. Mucoid M mutants of enteropathogenic (for suckling pigs), septicemic (for calves), and saprophytic E. Coli were subject of the study. The characteristics of the M colonies have been listed in Table 1. Both spontaneously occurring M mutants which had been isolated by culturing of E. coli strains (S form) in normal horse serum (undiluted) for several weeks were observed. Enzymatic-biochemical tests revealed the existence of M mutants with full enzmatic activity which are identical with the homologous S and R forms. There were also M mutants exhibiting a considerably reduced enzymatic activity. Antigenic-serological analysis (agglutination) exhibited M mutants which were agglutinated by the homologous sera, at low and high titres. In contrast to this, one group of M mutants was found not to be agglutinable, despite autoclaving at 120 degrees C for 2 hours. Some mutants cultured in broth for several weeks were re-exhibiting S colonies of full biochemical activity and full antigen reactivity against homologous agglutinating sera."}
{"id": "PMID:1101572", "title": "[Biochemical aspects of chronic rheumatic inflammation].", "content": "Morphological phenomena in rheumatoid arthritis are closely correlated to the biochemical aberrations of connective tissue metabolism. Both, morphological and biochemical analysis of the altered tissue portions demonstrate evidence for uncontrolled proliferation resp. metabolism similar to human and experimental malignoma. The present state of biochemical knowledge in this field permits to describe the metabolic state of the afflicted tissue by means of enzyme activity and substrate pattern and to draw some conclusions in respect to the pathogenesis of inflammatory processes involved in chronic rheumatic diseases.", "contents": "[Biochemical aspects of chronic rheumatic inflammation]. Morphological phenomena in rheumatoid arthritis are closely correlated to the biochemical aberrations of connective tissue metabolism. Both, morphological and biochemical analysis of the altered tissue portions demonstrate evidence for uncontrolled proliferation resp. metabolism similar to human and experimental malignoma. The present state of biochemical knowledge in this field permits to describe the metabolic state of the afflicted tissue by means of enzyme activity and substrate pattern and to draw some conclusions in respect to the pathogenesis of inflammatory processes involved in chronic rheumatic diseases."}
{"id": "PMID:1101579", "title": "[Swarming phenomenon of an aeromonas spec (author's transl)].", "content": "A genuine swarming phenomenon, such as has previously been known to occur in Proteus, Bacillus and Clostridium species only, was observed in an Aeromonas species. Fig. 1 shows the terraced swarming zones of the Aeromonas species on nutrient agar. The swarming rate, expressed as the growth of the swarming zone per time unit, was measured to be 70-120 mum/min on blood agar at 30 degrees C. The swarming could be inhibited by incubation at 37 degrees C (Table 2), by low saline concentrations (Table 3) as well as by addition of 4-nitro-phenylglycerol to the medium (Table 4). A DIENES-phenomenon between the swarming zones of Proteus strains and that of the Aeromonas species could not be observed (Fig. 2). The manner of swarming as seen in phase contrast microscopy was the same kind as that of Proteus. Furthermore, it could be shown by means of light- and electronmicroscopical investigations that the swarming phenomenon is connected with changes in the cell morphology and the form of flagellation (Figs. 4 and 5). Whereas in broth cultures (Fig. 3) as well as in the centre of colonies on solid media (Fig. 5a) the cells appeared as cocoid rods with polar flagellation, they developed elongated forms at the edge of the swarming zone, which - either in addition to or devoid of the polar flagella - were peritrichously populated with thin, flagella-like filaments (Figs. tb, 6, 7 and 8). The discussion deals with the various forms of bacterial surface translocation and investigates into the role of peritrichous flagella or fimbriae in the swarming phenomenon.", "contents": "[Swarming phenomenon of an aeromonas spec (author's transl)]. A genuine swarming phenomenon, such as has previously been known to occur in Proteus, Bacillus and Clostridium species only, was observed in an Aeromonas species. Fig. 1 shows the terraced swarming zones of the Aeromonas species on nutrient agar. The swarming rate, expressed as the growth of the swarming zone per time unit, was measured to be 70-120 mum/min on blood agar at 30 degrees C. The swarming could be inhibited by incubation at 37 degrees C (Table 2), by low saline concentrations (Table 3) as well as by addition of 4-nitro-phenylglycerol to the medium (Table 4). A DIENES-phenomenon between the swarming zones of Proteus strains and that of the Aeromonas species could not be observed (Fig. 2). The manner of swarming as seen in phase contrast microscopy was the same kind as that of Proteus. Furthermore, it could be shown by means of light- and electronmicroscopical investigations that the swarming phenomenon is connected with changes in the cell morphology and the form of flagellation (Figs. 4 and 5). Whereas in broth cultures (Fig. 3) as well as in the centre of colonies on solid media (Fig. 5a) the cells appeared as cocoid rods with polar flagellation, they developed elongated forms at the edge of the swarming zone, which - either in addition to or devoid of the polar flagella - were peritrichously populated with thin, flagella-like filaments (Figs. tb, 6, 7 and 8). The discussion deals with the various forms of bacterial surface translocation and investigates into the role of peritrichous flagella or fimbriae in the swarming phenomenon."}
{"id": "PMID:1101580", "title": "Minimal inhibitory dosage of rifampicin in intermittent treatment of Mycobacterium leprae infection in mice.", "content": "The total minimal inhibitory dose of rifampicin determined in the experimental mouse model, was found to be 10 mg/kg body weight, administered once a week for 6 weeks or once every 2 weeks for 12 weeks. From these and other results it is suggested that administration of RMP in human treatment can be reduced to a total amount of 7.2 either as a 600 mg dose once a week for 12 weeks or as a 900 mg dose once a week for 8 weeks. At present these regimens can only be used as an introductory treatment for multibacillary cases and are still too expensive for developing countries, but their efficacy should be evaluated in the field as sole treatments in tuberculoid cases, since they could signify a substantial economy for the management of the majority of leprosy infections.", "contents": "Minimal inhibitory dosage of rifampicin in intermittent treatment of Mycobacterium leprae infection in mice. The total minimal inhibitory dose of rifampicin determined in the experimental mouse model, was found to be 10 mg/kg body weight, administered once a week for 6 weeks or once every 2 weeks for 12 weeks. From these and other results it is suggested that administration of RMP in human treatment can be reduced to a total amount of 7.2 either as a 600 mg dose once a week for 12 weeks or as a 900 mg dose once a week for 8 weeks. At present these regimens can only be used as an introductory treatment for multibacillary cases and are still too expensive for developing countries, but their efficacy should be evaluated in the field as sole treatments in tuberculoid cases, since they could signify a substantial economy for the management of the majority of leprosy infections."}
{"id": "PMID:1101581", "title": "A comparison of tests for nitrate reduction.", "content": "504 strains of different bacteria have been tested for nitrate reduction by a conventional method and two microtests. A quick microtest for nitrate reduction is suggested allowing to read results after 1 hr cultivation. Nitrate reduction is a valuable test particularly for the species differentiation of the genera Bacillus, Micrococcus, Pseudomonas, Alcalignes, Moraxella, and other ones.", "contents": "A comparison of tests for nitrate reduction. 504 strains of different bacteria have been tested for nitrate reduction by a conventional method and two microtests. A quick microtest for nitrate reduction is suggested allowing to read results after 1 hr cultivation. Nitrate reduction is a valuable test particularly for the species differentiation of the genera Bacillus, Micrococcus, Pseudomonas, Alcalignes, Moraxella, and other ones."}
{"id": "PMID:1101582", "title": "[Quantitative description of the resistance of microbiological indicators by means of characteristic data (author's transl)].", "content": "The ambiguity of the quantitative description of the resistance of microbiological indicators by means of the terms common thus far, namely \"resistance\" (maximal period of action after which all indicators still exhibit organisms capable of reproduction) and \"death time\" (minimal period of action after which organisms capable of reproduction cannot be demonstrated any more in any of the indicators) is shown. Among other factors, the probability that all indicators will yield identical findings (sterility or growth of surviving organisms) at a defined time, is dependent upon the number of indicators in each case. The more indicators are tested, the lower \"resistance\" and the later the \"death time\" will be. Instead of times when all indicators present an identical reaction, times (or values related to these times in a defined way) should be taken at which indicators show the absence of organisms or the presence of surviving ones with a defined frequency. There is a defined relationship between the frequency of indicators found free from (resp. containing) surviving organisms and the number of surviving organisms per indicator (cf. Figs. 1 and 2). If the regularity of the decrease of organisms with the period of action of the destroying agent is known, 2 values are sufficient for a clear description of the resistance of a preparation of microbiological indicators. As characteristic values those values of action are proposed after which 99% and 1% of indicators will exhibit surviving organisms (t99% and t1%, respectively). The period during which the dependence of the frequency of presence of sterile indicators (po) upon the period of action of the destroying agent may be determined with sufficient precision and at justifiable experimental expenditure, is corresponding to a decrease of surviving organisms to appr. 1/30. From practical experience, it will be justified in the preponderant majority of cases to assume a logarithmic orderof death as given. Under such conditions, there will be a clear relationship between lg(-ln po) and the period of action of the destroying agent, so that test results may be easily evaluated by means of a simple graphical method or regression calculation. Graphical (FIG. 3) and mathematical determination of characteristic values from the frequency of indicators exhibitoring surviving organisms in the destruction test, after different periods of action, is explained by means of an example (Fig. 3; Tables 2 and 3) and a calculation (Table 1). To keep variation of experimental results sufficiently low, at least 30 (if possible 50) indicators per period of action should be studied for surviving organisms (cf. Fig 4). When evaluating results, different weights of the individual values and their asymmetric distribution around a maximum at t80% (Fig 5) should be taken into consideration.", "contents": "[Quantitative description of the resistance of microbiological indicators by means of characteristic data (author's transl)]. The ambiguity of the quantitative description of the resistance of microbiological indicators by means of the terms common thus far, namely \"resistance\" (maximal period of action after which all indicators still exhibit organisms capable of reproduction) and \"death time\" (minimal period of action after which organisms capable of reproduction cannot be demonstrated any more in any of the indicators) is shown. Among other factors, the probability that all indicators will yield identical findings (sterility or growth of surviving organisms) at a defined time, is dependent upon the number of indicators in each case. The more indicators are tested, the lower \"resistance\" and the later the \"death time\" will be. Instead of times when all indicators present an identical reaction, times (or values related to these times in a defined way) should be taken at which indicators show the absence of organisms or the presence of surviving ones with a defined frequency. There is a defined relationship between the frequency of indicators found free from (resp. containing) surviving organisms and the number of surviving organisms per indicator (cf. Figs. 1 and 2). If the regularity of the decrease of organisms with the period of action of the destroying agent is known, 2 values are sufficient for a clear description of the resistance of a preparation of microbiological indicators. As characteristic values those values of action are proposed after which 99% and 1% of indicators will exhibit surviving organisms (t99% and t1%, respectively). The period during which the dependence of the frequency of presence of sterile indicators (po) upon the period of action of the destroying agent may be determined with sufficient precision and at justifiable experimental expenditure, is corresponding to a decrease of surviving organisms to appr. 1/30. From practical experience, it will be justified in the preponderant majority of cases to assume a logarithmic orderof death as given. Under such conditions, there will be a clear relationship between lg(-ln po) and the period of action of the destroying agent, so that test results may be easily evaluated by means of a simple graphical method or regression calculation. Graphical (FIG. 3) and mathematical determination of characteristic values from the frequency of indicators exhibitoring surviving organisms in the destruction test, after different periods of action, is explained by means of an example (Fig. 3; Tables 2 and 3) and a calculation (Table 1). To keep variation of experimental results sufficiently low, at least 30 (if possible 50) indicators per period of action should be studied for surviving organisms (cf. Fig 4). When evaluating results, different weights of the individual values and their asymmetric distribution around a maximum at t80% (Fig 5) should be taken into consideration."}
{"id": "PMID:1101583", "title": "[Comparative studies of methods of salmonella enrichment (author's transl)].", "content": "Eight different methods of salmonella enrichment were compared in two series of experiments involving 100 samples of whole-egg powder and 80 samples of frozen whole liquid egg, respectively. 66 out of a total of 100 samples of whole-egg powder had been artificially infected with varying numbers of S. typhi-murium; 60 out of 80 samples of frozen whole liquid egg were found to be naturally infected with various salmonella species. 3 of the 8 methods (Table 1) were compared within an international collaborative study with 14 laboratories in 11 countries participating. A reduction of the pre-enrichment period from 18 to 6 hours and of volumes used in pre-enrichment and selective enrichment from 10 and 100 ml, respectively to 1 and 10 ml, respectively were found to have adverse influence upon the result of isolations, in particular in the case of weakly infected samples. In contrast, extended incubation over 48 hours as well as preparation of two sub-cultures on solid selective media following incubation of enrichment cultures over 18-24 hours and 42-48 hours, respectively always resulted in a certain increase of salmonella yield which, however, exhibited gradual differences for the individual methods examined. Preparation of a 2nd sub-culture meant, in particular, a decisive improvement of the result of isolations from artificially infected samples if selenite-cystine enrichment volumes were 10 and 100 ml, respectively. The best results could be obtained by means of the following methods of enrichment: Pre-enrichment of material in buffered peptone water at 37 degrees C over 18 hours; pipetting of 10 ml inoculated and incubated pre-enriched material into 100 ml selenite-cystine or tetrathionate enrichment medium according to MULLER-KAUFFMANN; onward incubation of the enrichment culture at 43 degrees C over 48 hours; and preparation of sub-cultures on solid selective media after 24 and 48 hours. The method using tetrathionate enrichment medium was found to be most expensive, results, however, were the most consistent ones.", "contents": "[Comparative studies of methods of salmonella enrichment (author's transl)]. Eight different methods of salmonella enrichment were compared in two series of experiments involving 100 samples of whole-egg powder and 80 samples of frozen whole liquid egg, respectively. 66 out of a total of 100 samples of whole-egg powder had been artificially infected with varying numbers of S. typhi-murium; 60 out of 80 samples of frozen whole liquid egg were found to be naturally infected with various salmonella species. 3 of the 8 methods (Table 1) were compared within an international collaborative study with 14 laboratories in 11 countries participating. A reduction of the pre-enrichment period from 18 to 6 hours and of volumes used in pre-enrichment and selective enrichment from 10 and 100 ml, respectively to 1 and 10 ml, respectively were found to have adverse influence upon the result of isolations, in particular in the case of weakly infected samples. In contrast, extended incubation over 48 hours as well as preparation of two sub-cultures on solid selective media following incubation of enrichment cultures over 18-24 hours and 42-48 hours, respectively always resulted in a certain increase of salmonella yield which, however, exhibited gradual differences for the individual methods examined. Preparation of a 2nd sub-culture meant, in particular, a decisive improvement of the result of isolations from artificially infected samples if selenite-cystine enrichment volumes were 10 and 100 ml, respectively. The best results could be obtained by means of the following methods of enrichment: Pre-enrichment of material in buffered peptone water at 37 degrees C over 18 hours; pipetting of 10 ml inoculated and incubated pre-enriched material into 100 ml selenite-cystine or tetrathionate enrichment medium according to MULLER-KAUFFMANN; onward incubation of the enrichment culture at 43 degrees C over 48 hours; and preparation of sub-cultures on solid selective media after 24 and 48 hours. The method using tetrathionate enrichment medium was found to be most expensive, results, however, were the most consistent ones."}
{"id": "PMID:1101584", "title": "[Utilization of LIN (lysine-indole-motility) medium for the preliminary identification of enteropathogenic bacteria].", "content": "A multiple-test medium for the routine laboratory identification of enteropathogenic bacteria is described. The medium has the following formula: Bacto-peptone (Difco) 5 g; yeast extract (Difco) 3 g; casein tryptic digest peptone (Merek) 15 g; glucose 1 g; L-lysine-monohydrochloride (Merck, No. 5700) 5 g; NaCl 5 g; bromcresol purple 0.016 g; agar 2 g; distilled water 1000 ml; final pH 6.6. The medium is dispensed in amounts of 5 ml into tubes of 14 X 85 mm and autoclaved at 120 degrees C for 10 min. The tubes are tightly closed with rubber stoppers. - The medium is inoculated by stabbing to the bottom of the tube. Readings are made after over-night incubation at 37 degrees C. A scheme for the preliminary identification of enteropathogenic bacteria is given, based on LIM medium in conjunction with Kligler's iron agar, and the oxidase reaction.", "contents": "[Utilization of LIN (lysine-indole-motility) medium for the preliminary identification of enteropathogenic bacteria]. A multiple-test medium for the routine laboratory identification of enteropathogenic bacteria is described. The medium has the following formula: Bacto-peptone (Difco) 5 g; yeast extract (Difco) 3 g; casein tryptic digest peptone (Merek) 15 g; glucose 1 g; L-lysine-monohydrochloride (Merck, No. 5700) 5 g; NaCl 5 g; bromcresol purple 0.016 g; agar 2 g; distilled water 1000 ml; final pH 6.6. The medium is dispensed in amounts of 5 ml into tubes of 14 X 85 mm and autoclaved at 120 degrees C for 10 min. The tubes are tightly closed with rubber stoppers. - The medium is inoculated by stabbing to the bottom of the tube. Readings are made after over-night incubation at 37 degrees C. A scheme for the preliminary identification of enteropathogenic bacteria is given, based on LIM medium in conjunction with Kligler's iron agar, and the oxidase reaction."}
{"id": "PMID:1101585", "title": "[The application of chorioallantois membranes of chicken embryos for the virulence determination of Nocardia asteroides (author's transl)].", "content": "The pathogenicity of Nocardia asteroides and other strains of different Nocardia species against chicken embryos was investigated. The strains were inoculated on the chorioallantois membrane of embryonated eggs and the resulting lesions were judged. 55 Nocardia asteroides strains and some strains of the species N. brasiliensis, N. leishmanii, N. caviae, N. (Actinomadura) madurae, N. caprae, N. rubra, N. pellegrini, N. corallina and N. polychromogenes were studied. 47 strains of the species N. asteroides were pathogenic, 2 apathogenic and 6 strains showed doubtful results. All the investigated 5 strains of the species N. brasiliensis, N. leishmanii, N. caviae and N. (Actinomadura) madurae, provoked typical lesions on the chorioallantois membrane. The other investigated strains were apathogenic. With this, described test it is possible to distinguish between pathogenic and apathogenic Nocardia strains, which is indeed important for the diagnosis of Nocardia infection.", "contents": "[The application of chorioallantois membranes of chicken embryos for the virulence determination of Nocardia asteroides (author's transl)]. The pathogenicity of Nocardia asteroides and other strains of different Nocardia species against chicken embryos was investigated. The strains were inoculated on the chorioallantois membrane of embryonated eggs and the resulting lesions were judged. 55 Nocardia asteroides strains and some strains of the species N. brasiliensis, N. leishmanii, N. caviae, N. (Actinomadura) madurae, N. caprae, N. rubra, N. pellegrini, N. corallina and N. polychromogenes were studied. 47 strains of the species N. asteroides were pathogenic, 2 apathogenic and 6 strains showed doubtful results. All the investigated 5 strains of the species N. brasiliensis, N. leishmanii, N. caviae and N. (Actinomadura) madurae, provoked typical lesions on the chorioallantois membrane. The other investigated strains were apathogenic. With this, described test it is possible to distinguish between pathogenic and apathogenic Nocardia strains, which is indeed important for the diagnosis of Nocardia infection."}
{"id": "PMID:1101586", "title": "[Phagocytosis and intracellular proliferation of Nocardia asteroides (strain Weipheld) in cell structures in vitro. 1. Alveolar macrophages of guinea-pigs (author's transl)].", "content": "Phagocytosis and intracellular proliferation of Nocardia asteroides (Weipheld) were studied in alveolar macrophages from normal guinea-pigs that had been immunized in two stages and guinea-pigs that had undergone long-term cortisone treatment (28 mg/kg/day over 30 days). The highest phagocytosis values were found in the guinea-pigs that had been immunized in two stages (3.85-6.82%). In the case of normal macrophages, values of 2.90-4.95% were observed. The lowest values (2.10-3.6%) were found for macrophages from those guinea-pigs that had been treated with hydrocortisone. Differences between the groups were statistically significant. Following phagocytosis, the phagocytosis index was seen rising in all three groups, reaching its highest values for alveolar macrophages from the cortisone-treated guinea-pigs (after 8 hours - 1.15). The cytotoxic effects of Nocardia upon the cells in the lung washing liquid from normal guinea-pigs immunized in two steps and guinea-pigs treated with high cortisone doses over an extended period were exhibiting identical trends. After 8 hours' exposure, 22.6% dead and decayed cells were found, against 5.9% in normal controls. Leucocytes proved to be most susceptible to the cytotoxic effect of Nocardia; alveolar macrophages were less and small lymphocytes least susceptible.", "contents": "[Phagocytosis and intracellular proliferation of Nocardia asteroides (strain Weipheld) in cell structures in vitro. 1. Alveolar macrophages of guinea-pigs (author's transl)]. Phagocytosis and intracellular proliferation of Nocardia asteroides (Weipheld) were studied in alveolar macrophages from normal guinea-pigs that had been immunized in two stages and guinea-pigs that had undergone long-term cortisone treatment (28 mg/kg/day over 30 days). The highest phagocytosis values were found in the guinea-pigs that had been immunized in two stages (3.85-6.82%). In the case of normal macrophages, values of 2.90-4.95% were observed. The lowest values (2.10-3.6%) were found for macrophages from those guinea-pigs that had been treated with hydrocortisone. Differences between the groups were statistically significant. Following phagocytosis, the phagocytosis index was seen rising in all three groups, reaching its highest values for alveolar macrophages from the cortisone-treated guinea-pigs (after 8 hours - 1.15). The cytotoxic effects of Nocardia upon the cells in the lung washing liquid from normal guinea-pigs immunized in two steps and guinea-pigs treated with high cortisone doses over an extended period were exhibiting identical trends. After 8 hours' exposure, 22.6% dead and decayed cells were found, against 5.9% in normal controls. Leucocytes proved to be most susceptible to the cytotoxic effect of Nocardia; alveolar macrophages were less and small lymphocytes least susceptible."}
{"id": "PMID:1101587", "title": "Selective involvement of the brain in experimental murine cryptococcosis. I. Microbiological observations.", "content": "During the course of infection in intraperitoneally infected white mice with a particular strain of Cryptococcus neoformans, it was possible to observe a time-limited and selective presence of the fungus in the brain. The presence of the fungus in the brain without causing clinical symptoms and the rare mortal exacerbation of the brain involvement offers possibilities for comparative studies of experimental and human cryptococcosis. On the basis of these strain-specific results new aspects of the virulence of C. neoformans and the epidemiology of cryptococcosis are discussed.", "contents": "Selective involvement of the brain in experimental murine cryptococcosis. I. Microbiological observations. During the course of infection in intraperitoneally infected white mice with a particular strain of Cryptococcus neoformans, it was possible to observe a time-limited and selective presence of the fungus in the brain. The presence of the fungus in the brain without causing clinical symptoms and the rare mortal exacerbation of the brain involvement offers possibilities for comparative studies of experimental and human cryptococcosis. On the basis of these strain-specific results new aspects of the virulence of C. neoformans and the epidemiology of cryptococcosis are discussed."}
{"id": "PMID:1101588", "title": "[Role of chronic toxoplasmosis infection in pathology of the nervous system and eyes in adults].", "content": "The authors conducted a statistical correlation of frequencies of neurological and ophthalmological pathology in a group of patients with different disorders of the nervous system, where 556 had positive and 788 negative seroallergical reactions to toxoplasmosis. It was possible to establish that most of the neurological and ophthalmological symptoms of toxoplasmosis were significantly more frequent in individuals of the first group, except patients with disseminated sclerosis, myopathy and myasthenia.", "contents": "[Role of chronic toxoplasmosis infection in pathology of the nervous system and eyes in adults]. The authors conducted a statistical correlation of frequencies of neurological and ophthalmological pathology in a group of patients with different disorders of the nervous system, where 556 had positive and 788 negative seroallergical reactions to toxoplasmosis. It was possible to establish that most of the neurological and ophthalmological symptoms of toxoplasmosis were significantly more frequent in individuals of the first group, except patients with disseminated sclerosis, myopathy and myasthenia."}
{"id": "PMID:1101589", "title": "[Place of the clinic in studying the nature of schizophrenia].", "content": "For successful international studies it is necessary to overcome differences in national psychiatry that are conditioned by the existing clinical methods of research. However, this method is necessary for a cognition of the substrate of a disease as it depicts regularities of clinical expressions of a disease. An example of that is given by Jackson who displayed a correlation between the positive and negative symptoms in a psychosis. This concept is considered from the point of view of relations between adaptive reactions of the organism and a breakage (Pavlov, Davidovsky) and in connection with the nozological problems.", "contents": "[Place of the clinic in studying the nature of schizophrenia]. For successful international studies it is necessary to overcome differences in national psychiatry that are conditioned by the existing clinical methods of research. However, this method is necessary for a cognition of the substrate of a disease as it depicts regularities of clinical expressions of a disease. An example of that is given by Jackson who displayed a correlation between the positive and negative symptoms in a psychosis. This concept is considered from the point of view of relations between adaptive reactions of the organism and a breakage (Pavlov, Davidovsky) and in connection with the nozological problems."}
{"id": "PMID:1101590", "title": "[Lithium prophylaxis].", "content": "The author considers some difficulties and discrepancies in the diagnosis of schizophrenia, schizo-affective psychoses on the one hand and manic-depressive psychosis on the other in a group of out-patients receiving treatment with lithium for the prophylaxis of affective disorders. Factors common to both groups and being the basis for the selection of lithium prophylaxis are considered. Special attention in these cases is paid to the course of the disease.", "contents": "[Lithium prophylaxis]. The author considers some difficulties and discrepancies in the diagnosis of schizophrenia, schizo-affective psychoses on the one hand and manic-depressive psychosis on the other in a group of out-patients receiving treatment with lithium for the prophylaxis of affective disorders. Factors common to both groups and being the basis for the selection of lithium prophylaxis are considered. Special attention in these cases is paid to the course of the disease."}
{"id": "PMID:1101592", "title": "Homolanthionine in fungi: accumulation in the methionine-requiring mutants of Aspergillus nidulans.", "content": "Homolanthionine, a higher homologue of cystathionine, was found to accumulate in the mutants of Aspergillus nidulan impaired in the synthesis of methionine from homocysteine. The additional introduction of mutation resulting in a block at cystathionine gamma-synthase but not at cystathionine beta-synthase abolishes accumulation of both homolanthionine and cystathionine. This suggests a possible participation of cystathionine gamma-synthase in homolanthionine synthesis.", "contents": "Homolanthionine in fungi: accumulation in the methionine-requiring mutants of Aspergillus nidulans. Homolanthionine, a higher homologue of cystathionine, was found to accumulate in the mutants of Aspergillus nidulan impaired in the synthesis of methionine from homocysteine. The additional introduction of mutation resulting in a block at cystathionine gamma-synthase but not at cystathionine beta-synthase abolishes accumulation of both homolanthionine and cystathionine. This suggests a possible participation of cystathionine gamma-synthase in homolanthionine synthesis."}
{"id": "PMID:1101593", "title": "[Influence of BCG on the production of antibody-forming cells in the primary and secondary immune response against sheep erythrocytes in the mouse].", "content": "Adult mice react with an increased production of PFC (plaque-forming cells) against sheep erythrocytes (SE). in the primary immune response after application of BCG. Animals receiving 0,5 mg of BCG 10 days before SE show an increase in direct PFC of more than 50%. No effect was observed after injecting BCG 14 or 5 days before SE or using a dose of 0,1 mg of BCG. The results indicate that the effect of BCG on the humoral immune response is influenced by the interval between application of BCG and the SE as well as the BCG-dose. By contrast, BCG had no significant influence on the secondary humoral immune response. In a few cases the immune response seemed to be suppressed.", "contents": "[Influence of BCG on the production of antibody-forming cells in the primary and secondary immune response against sheep erythrocytes in the mouse]. Adult mice react with an increased production of PFC (plaque-forming cells) against sheep erythrocytes (SE). in the primary immune response after application of BCG. Animals receiving 0,5 mg of BCG 10 days before SE show an increase in direct PFC of more than 50%. No effect was observed after injecting BCG 14 or 5 days before SE or using a dose of 0,1 mg of BCG. The results indicate that the effect of BCG on the humoral immune response is influenced by the interval between application of BCG and the SE as well as the BCG-dose. By contrast, BCG had no significant influence on the secondary humoral immune response. In a few cases the immune response seemed to be suppressed."}
{"id": "PMID:1101595", "title": "Central venous catheterization through the basilic vein or by infraclavicular puncture? A controlled trial.", "content": "Central venous catheterization through the basilic vein and by infraclavicular puncture of the subclavian vein was compared in a trial with random allocation to 125 candidate patients. The catheters were inserted via the two routes with roughly equal success rates. Subclavian catheters were maintained better for the period required than were basilic catheters. The infraclavicular puncture caused initial, and basilic catheterization, late complications. Consequently, infraclavicular puncture is preferable for long-term, and basilic catheterization for short-term, needs.", "contents": "Central venous catheterization through the basilic vein or by infraclavicular puncture? A controlled trial. Central venous catheterization through the basilic vein and by infraclavicular puncture of the subclavian vein was compared in a trial with random allocation to 125 candidate patients. The catheters were inserted via the two routes with roughly equal success rates. Subclavian catheters were maintained better for the period required than were basilic catheters. The infraclavicular puncture caused initial, and basilic catheterization, late complications. Consequently, infraclavicular puncture is preferable for long-term, and basilic catheterization for short-term, needs."}
{"id": "PMID:1101596", "title": "Surgical treatment of 102 tendo achillis ruptures-- suture or tenontoplasty?", "content": "A series of 102 patients with total subcutaneous rupture of the achilles tendon treated surgically from 1944 to 1972 is presneted. All patients were treated early after the acute event, and the diagnosis was made on clinical examination. Group I comprises 54 patients treated over the first part of the period by simple tendon suture, and group II comprises 48 patients from the second part of the period treated by tenontoplasty ad modum Silfverskj\u00f6ld. The results in the two groups are compared and also contrasted with the results following conservative treatment more recently reported. Operative treatment is recommended rather than conservative bandaging. There were no recurrent ruptures in group II, but 2 patients in group I had rupture recurrence. The functional results were not statistically significantly different in the two groups. There was no operative mortality. Surgical complications were infrequent, and, in general, did not affect the period of treatment, nor the duration of incapacity. Recent studies reported in the literature cast doubt on frequent previous reports of pathological changes in the tendon predisposing to rupture. Some constitutional weakness would appear to be etiological from this study where some familial incidence was noted, and particularly there was an incidence of not simultaneous bilateral rupture. The risk of contralateral rupture if patients returned to sporting activity to the same extent as before the primary injury was 26% (12-45%).", "contents": "Surgical treatment of 102 tendo achillis ruptures-- suture or tenontoplasty? A series of 102 patients with total subcutaneous rupture of the achilles tendon treated surgically from 1944 to 1972 is presneted. All patients were treated early after the acute event, and the diagnosis was made on clinical examination. Group I comprises 54 patients treated over the first part of the period by simple tendon suture, and group II comprises 48 patients from the second part of the period treated by tenontoplasty ad modum Silfverskj\u00f6ld. The results in the two groups are compared and also contrasted with the results following conservative treatment more recently reported. Operative treatment is recommended rather than conservative bandaging. There were no recurrent ruptures in group II, but 2 patients in group I had rupture recurrence. The functional results were not statistically significantly different in the two groups. There was no operative mortality. Surgical complications were infrequent, and, in general, did not affect the period of treatment, nor the duration of incapacity. Recent studies reported in the literature cast doubt on frequent previous reports of pathological changes in the tendon predisposing to rupture. Some constitutional weakness would appear to be etiological from this study where some familial incidence was noted, and particularly there was an incidence of not simultaneous bilateral rupture. The risk of contralateral rupture if patients returned to sporting activity to the same extent as before the primary injury was 26% (12-45%)."}
{"id": "PMID:1101598", "title": "Purification and characterisation of two porcine hypothalamic fractions with LH-releasing activity: evidence for a single LH and FSH-releasing hormone.", "content": "Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH-RH and synthetic LH-RH, E 203 was identified as purified natural LH-RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH-releasing activity as natural LH-RH. The binding to a specific LH-RH antiserum is identical with that of natural LH-RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH-RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.", "contents": "Purification and characterisation of two porcine hypothalamic fractions with LH-releasing activity: evidence for a single LH and FSH-releasing hormone. Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH-RH and synthetic LH-RH, E 203 was identified as purified natural LH-RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH-releasing activity as natural LH-RH. The binding to a specific LH-RH antiserum is identical with that of natural LH-RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH-RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions."}
{"id": "PMID:1101597", "title": "Studies on the duct-ligated pancreas as allograft for islet cell function.", "content": "Twenty previously pancreatectomized dogs were heterotopically transplanted with duct-ligated allografts. Sixteen of the grafts were preserved for 24 hours, nine with continuous hypothermic albumin perfusion, seven with hypothermic storage. Five dogs died from early postoperative bleedings. Fifteen surviving dogs had a satisfying endocrine function assessed by blood glucose and serum insulin during fasting and after glucose load. In six dogs the glucagon release from the graft was investigated and significant release was evident in all dogs. A high release was found after L-arginine stimulation. The endocrine function was satisfying after 24 hour preservation both with hypothermic storage and continuous hypothermic albumin perfusion. During such perfusion the pancreas became very oedematous. This did not influence on the outcome of the transplantation. The grafts were enlarged four to eight days after transplantation. Oedema, fibrotic changes and collagen depositions in the interstitial tissue were observed in light microscopy. These changes seemed to come earlier in glands preserved for 24 hours. Islet cells were identified in all functioning grafts and seemed histopathologically and ultrastructurally unchanged. A duct-ligated pancreatic segment was allografted to one diabetic patient in a pilot experiment. There was a satisfying endocrine function for 40 days. After that the graft became necrotic due to thrombus formation.", "contents": "Studies on the duct-ligated pancreas as allograft for islet cell function. Twenty previously pancreatectomized dogs were heterotopically transplanted with duct-ligated allografts. Sixteen of the grafts were preserved for 24 hours, nine with continuous hypothermic albumin perfusion, seven with hypothermic storage. Five dogs died from early postoperative bleedings. Fifteen surviving dogs had a satisfying endocrine function assessed by blood glucose and serum insulin during fasting and after glucose load. In six dogs the glucagon release from the graft was investigated and significant release was evident in all dogs. A high release was found after L-arginine stimulation. The endocrine function was satisfying after 24 hour preservation both with hypothermic storage and continuous hypothermic albumin perfusion. During such perfusion the pancreas became very oedematous. This did not influence on the outcome of the transplantation. The grafts were enlarged four to eight days after transplantation. Oedema, fibrotic changes and collagen depositions in the interstitial tissue were observed in light microscopy. These changes seemed to come earlier in glands preserved for 24 hours. Islet cells were identified in all functioning grafts and seemed histopathologically and ultrastructurally unchanged. A duct-ligated pancreatic segment was allografted to one diabetic patient in a pilot experiment. There was a satisfying endocrine function for 40 days. After that the graft became necrotic due to thrombus formation."}
{"id": "PMID:1101599", "title": "Acute effects of exogenous growth hormone in man: time- and dose-bound modification of glucose tolerance and glucose-induced insulin release.", "content": "The time and dose dependency of the effects of a 30-min long iv infusion of human growth hormone (GH) on glucose tolerance and glucose-stimulated insulin release was investigated in 19 healthy subjects. Glucose tolerance deteriorated immediately following GH, and the k-value continued to decrease up to 300 min later. A small but significant reduction of glucose tolerance persisted 24 h after GH administration. Significant deterioration of glucose tolerance was observed with the smallest GH dose used (5 mug per kg body weight), increasing the amount of the hormone having no further major influence. Glucose-stimulated insulin release was significantly inhibited 1 h after administration of a relatively high GH dose (40 mug per kg), both if expressed as mean plasma insulin levels, or as insulin release per magnitude of glucose stimulation (insulinogenic index). In the majority of subjects, insulin release was inhibited also by lower GH doses (5-20 mug GH per kg). However, the mean change with these doses was not statistically significant. The inhibitory effect of GH on insulin secretion seemed to have a duration of several hours. Five hours, but not 24 h, after GH administration (10 mug GH per kg) insulin release was still significantly suppressed. It is suggested that the initial effect of GH on pancreatic beta cells may be inhibition of insulin release, in contrast with the enhancement of insulin secretion observed during chronic administration of GH.", "contents": "Acute effects of exogenous growth hormone in man: time- and dose-bound modification of glucose tolerance and glucose-induced insulin release. The time and dose dependency of the effects of a 30-min long iv infusion of human growth hormone (GH) on glucose tolerance and glucose-stimulated insulin release was investigated in 19 healthy subjects. Glucose tolerance deteriorated immediately following GH, and the k-value continued to decrease up to 300 min later. A small but significant reduction of glucose tolerance persisted 24 h after GH administration. Significant deterioration of glucose tolerance was observed with the smallest GH dose used (5 mug per kg body weight), increasing the amount of the hormone having no further major influence. Glucose-stimulated insulin release was significantly inhibited 1 h after administration of a relatively high GH dose (40 mug per kg), both if expressed as mean plasma insulin levels, or as insulin release per magnitude of glucose stimulation (insulinogenic index). In the majority of subjects, insulin release was inhibited also by lower GH doses (5-20 mug GH per kg). However, the mean change with these doses was not statistically significant. The inhibitory effect of GH on insulin secretion seemed to have a duration of several hours. Five hours, but not 24 h, after GH administration (10 mug GH per kg) insulin release was still significantly suppressed. It is suggested that the initial effect of GH on pancreatic beta cells may be inhibition of insulin release, in contrast with the enhancement of insulin secretion observed during chronic administration of GH."}
{"id": "PMID:1101600", "title": "Radioimmunoassay of human prolactin (PRL).", "content": "A simple and rapid homologous radioimmunoassay of human prolactin (hPRL), by the use of 66% ethanol containing 6.6% ammonium acetate for the separation of free and bound hormones, has been established. PRL levels showed marked individual and daily variations as well as a circadian periodicity in both men and women. Levels of plasma PRL rose with puberty and decreased during post-menopause and in elderly men (ages 60-73 years). There was no marked change in plasma PRL levels at midcycle; however, in some subjects, luteal levels were slightly higher than follicular levels. Administration of oestradiol-17beta caused marked increase in plasma PRL levels during menstrual cycle. During pregnancy, the plasma PRL levels rose as high as 200 ng/ml and decreased within a week or two post-partum. During lactation, the levels were higher and increased with suckling. In a patient with habitual abortion, the plasma PRL level showed a sudden drop prior to abortion. Administration of LH-RH had no effect on the secretion of PRL in human subjects.", "contents": "Radioimmunoassay of human prolactin (PRL). A simple and rapid homologous radioimmunoassay of human prolactin (hPRL), by the use of 66% ethanol containing 6.6% ammonium acetate for the separation of free and bound hormones, has been established. PRL levels showed marked individual and daily variations as well as a circadian periodicity in both men and women. Levels of plasma PRL rose with puberty and decreased during post-menopause and in elderly men (ages 60-73 years). There was no marked change in plasma PRL levels at midcycle; however, in some subjects, luteal levels were slightly higher than follicular levels. Administration of oestradiol-17beta caused marked increase in plasma PRL levels during menstrual cycle. During pregnancy, the plasma PRL levels rose as high as 200 ng/ml and decreased within a week or two post-partum. During lactation, the levels were higher and increased with suckling. In a patient with habitual abortion, the plasma PRL level showed a sudden drop prior to abortion. Administration of LH-RH had no effect on the secretion of PRL in human subjects."}
{"id": "PMID:1101601", "title": "Response to pentagastrin in man. III. Comparison of two formulations.", "content": "Gastric secretion has been studied in response to the standard preparation of pentagastrin and a new, more stable preparation (pentagastrin meglumine). The sensitivity to, and maximal outputs of acid and pepsin elicited by, the two preparations of pentagastrin did not differ. However, the pentagastrin meglumine evoked a better sustained acid secretion with all dose rates than did the standard pentagastrin.", "contents": "Response to pentagastrin in man. III. Comparison of two formulations. Gastric secretion has been studied in response to the standard preparation of pentagastrin and a new, more stable preparation (pentagastrin meglumine). The sensitivity to, and maximal outputs of acid and pepsin elicited by, the two preparations of pentagastrin did not differ. However, the pentagastrin meglumine evoked a better sustained acid secretion with all dose rates than did the standard pentagastrin."}
{"id": "PMID:1101602", "title": "Evaluation of impedance cardiography as a non-invasive means of measuring systolic time intervals and cardiac output.", "content": "Impedance cardiography was used for non-invasive determinations of systolic time intervals (STI) and cardiac output. The results were compared with simultaneously obtained invasive measurements of STI from central aortic pressure curves and of cardiac output using the dye-dilution technique. The study was performed on eight dogs during increasing halothane concentration. A close correlation was found between non-invasively and invasively measured left ventricular ejection time = LVET (r = 0.986) and pre-ejection period - PEP (r - 0.948). Measurements of cardiac output derived from changes in thoracic impedance were determined 1) using a fixed value of p (p - the resistivity of blood) and 2) using an individual value of p based on the actual hematocrits. When compared to cardiac outputs obtained by dye-dilution the correlation coefficients were r = 0.806 and r = 0.816, respectively. Impedance cardiography is a useful method of evaluating changes in cardiac output. The method permits simultaneous observations of changes in STI and cardiac output as an index of cardiac function.", "contents": "Evaluation of impedance cardiography as a non-invasive means of measuring systolic time intervals and cardiac output. Impedance cardiography was used for non-invasive determinations of systolic time intervals (STI) and cardiac output. The results were compared with simultaneously obtained invasive measurements of STI from central aortic pressure curves and of cardiac output using the dye-dilution technique. The study was performed on eight dogs during increasing halothane concentration. A close correlation was found between non-invasively and invasively measured left ventricular ejection time = LVET (r = 0.986) and pre-ejection period - PEP (r - 0.948). Measurements of cardiac output derived from changes in thoracic impedance were determined 1) using a fixed value of p (p - the resistivity of blood) and 2) using an individual value of p based on the actual hematocrits. When compared to cardiac outputs obtained by dye-dilution the correlation coefficients were r = 0.806 and r = 0.816, respectively. Impedance cardiography is a useful method of evaluating changes in cardiac output. The method permits simultaneous observations of changes in STI and cardiac output as an index of cardiac function."}
{"id": "PMID:1101603", "title": "Paracervical block with etidocaine for out-patient abortion.", "content": "Paracervical block using etidocaine 0.5 % and 1.0 % without adrenaline proved to be an effective anaesthetic procedure for first trimester abortion performed by surgical dilatation of the cervix and subsequent vacuum suction. Three blocks out of 24 were inadequate, probably due to inaccurate deposition of the local anaesthetic. There were two adverse reactions, possibly as a result of inadvertant intravascular injection. The need for long-acting agents for the present indication is questioned.", "contents": "Paracervical block with etidocaine for out-patient abortion. Paracervical block using etidocaine 0.5 % and 1.0 % without adrenaline proved to be an effective anaesthetic procedure for first trimester abortion performed by surgical dilatation of the cervix and subsequent vacuum suction. Three blocks out of 24 were inadequate, probably due to inaccurate deposition of the local anaesthetic. There were two adverse reactions, possibly as a result of inadvertant intravascular injection. The need for long-acting agents for the present indication is questioned."}
{"id": "PMID:1101604", "title": "Acute intravenous tolerance of etidocaine.", "content": "Etidocaine can be said to be midway between lidocaine and bupivacaine in respect to its toxicity. Its therapeutic ratio will depend on a more exact comparison of the clinical efficacy of the three agents.", "contents": "Acute intravenous tolerance of etidocaine. Etidocaine can be said to be midway between lidocaine and bupivacaine in respect to its toxicity. Its therapeutic ratio will depend on a more exact comparison of the clinical efficacy of the three agents."}
{"id": "PMID:1101606", "title": "A comparative study between etidocaine and bupivacaine in ulnar nerve block.", "content": "The long-acting local anaesthetics bupivacaine and etidocaine were compared in a double-blind study using ulnar nerve block. Eleven volunteers took part in the study. Solutions used were: bupivacaine 0.25% and 0.5% and etidocaine 0.5% and 1 %. All solutions contained adrenaline 5 mug/ml. The time of onset and duration of analgesia and motor block, degree of motor block, and changes in skin temperature were investigated. The results did not show any differences in the duration of sensory of motor block. Differences were seen in the degree of motor block in favour of etidocaine. The sympathetic block seemed to be more intense and of longer duration with bupivacaine. Etidocaine had a shorter time of onset in all nerve fibres.", "contents": "A comparative study between etidocaine and bupivacaine in ulnar nerve block. The long-acting local anaesthetics bupivacaine and etidocaine were compared in a double-blind study using ulnar nerve block. Eleven volunteers took part in the study. Solutions used were: bupivacaine 0.25% and 0.5% and etidocaine 0.5% and 1 %. All solutions contained adrenaline 5 mug/ml. The time of onset and duration of analgesia and motor block, degree of motor block, and changes in skin temperature were investigated. The results did not show any differences in the duration of sensory of motor block. Differences were seen in the degree of motor block in favour of etidocaine. The sympathetic block seemed to be more intense and of longer duration with bupivacaine. Etidocaine had a shorter time of onset in all nerve fibres."}
{"id": "PMID:1101608", "title": "Axillary plexus block with etidocaine.", "content": "Axillary plexus block has been used to compare 0.5% and 1.0% etidocaine (with adrenaline 5 mug/ml) in 32 patients in an open label study. The injected volume was 30 ml. The time to total analgesia and motor block was around 10-12 minutes. Complete block was achieved in more than 97% of cases. The duration of total analgesia and motor block was 6-7 hours. The time from injection to return of total sensation and motility was 8.5-10 hours. Adequate analgesia was achieved in all patients but two (one in each group). No noteworthy differences were found between the two solutions.", "contents": "Axillary plexus block with etidocaine. Axillary plexus block has been used to compare 0.5% and 1.0% etidocaine (with adrenaline 5 mug/ml) in 32 patients in an open label study. The injected volume was 30 ml. The time to total analgesia and motor block was around 10-12 minutes. Complete block was achieved in more than 97% of cases. The duration of total analgesia and motor block was 6-7 hours. The time from injection to return of total sensation and motility was 8.5-10 hours. Adequate analgesia was achieved in all patients but two (one in each group). No noteworthy differences were found between the two solutions."}
{"id": "PMID:1101609", "title": "Supraclavicular brachial plexus block with etidocaine without vasoconstrictor. Preliminary report.", "content": "When used for supraclavicular brachial plexus blocks, etidocaine 1% gave a quick and sufficient onset. Its duration was longer than that of mepivacaine 2%, and equal to that of bupivacaine 0.5%. Onset times for etidocaine 0.5% and bupivacaine 0.5% were about equal, but the former seemed to give a more profound motor block. The duration of etidocaine 0.5% was shorter than that of bupivacaine 0.5%. The long-acting agents showed great variations in duration, and they all had longer regression times than mepivacaine. No serious pharmacological side-effects were observed during this study, and so far there are no reports on neurological sequelae.", "contents": "Supraclavicular brachial plexus block with etidocaine without vasoconstrictor. Preliminary report. When used for supraclavicular brachial plexus blocks, etidocaine 1% gave a quick and sufficient onset. Its duration was longer than that of mepivacaine 2%, and equal to that of bupivacaine 0.5%. Onset times for etidocaine 0.5% and bupivacaine 0.5% were about equal, but the former seemed to give a more profound motor block. The duration of etidocaine 0.5% was shorter than that of bupivacaine 0.5%. The long-acting agents showed great variations in duration, and they all had longer regression times than mepivacaine. No serious pharmacological side-effects were observed during this study, and so far there are no reports on neurological sequelae."}
{"id": "PMID:1101610", "title": "Intercostal blocks with etidocaine. Preliminary report.", "content": "Although the results are few and should be considered as preliminary, the following conclusions may be drawn: 1. Etidocaine 0.5% and bupivacaine 0.5%, both with adrenaline (5 mug/ml), are comparable with regard to duration of sensory block. 2. Etidocaine 0.5% without adrenaline did not give the same duration of complete sensory block as when adrenaline was present. 3. Etidocaine 0.5% acts as a vasodilator at the site of injection. Adrenaline added to the solution counteracts the dilating effect. 4. Blood level studies of etidocaine do not at present show differences when 0.5% solution with or without adrenaline is used.", "contents": "Intercostal blocks with etidocaine. Preliminary report. Although the results are few and should be considered as preliminary, the following conclusions may be drawn: 1. Etidocaine 0.5% and bupivacaine 0.5%, both with adrenaline (5 mug/ml), are comparable with regard to duration of sensory block. 2. Etidocaine 0.5% without adrenaline did not give the same duration of complete sensory block as when adrenaline was present. 3. Etidocaine 0.5% acts as a vasodilator at the site of injection. Adrenaline added to the solution counteracts the dilating effect. 4. Blood level studies of etidocaine do not at present show differences when 0.5% solution with or without adrenaline is used."}
{"id": "PMID:1101611", "title": "Comparative study with etidocaine and bupivacaine in epidural block.", "content": "Etidocaine 1% and bupivacaine 0.5% (both with adrenaline 5 mug/ml) have been compared in a double blind study in epidural analgesia. Time of onset, duration, recession of analgesia and intensity as well as frequency of motor blockade were recorded in a selected group of patients undergoing varicose vein stripping. The onset of action of etidocaine was more rapid. Duration, recession and intensity of analgesia were similar for the two drugs. The main difference between them was that etidocaine produced a higher frequency and greater intensity of motor blockade.", "contents": "Comparative study with etidocaine and bupivacaine in epidural block. Etidocaine 1% and bupivacaine 0.5% (both with adrenaline 5 mug/ml) have been compared in a double blind study in epidural analgesia. Time of onset, duration, recession of analgesia and intensity as well as frequency of motor blockade were recorded in a selected group of patients undergoing varicose vein stripping. The onset of action of etidocaine was more rapid. Duration, recession and intensity of analgesia were similar for the two drugs. The main difference between them was that etidocaine produced a higher frequency and greater intensity of motor blockade."}
{"id": "PMID:1101614", "title": "Comparison between bupivacaine and etidocaine in lumbar epidural block for urological surgery.", "content": "Lumbar epidural blocks produced by 17 ml of etidocaine 1% or bupivacaine 0.5%, both with adrenaline (5 mug/ml), have been compared in a double-blind trial in 40 patients, all scheduled for elective urological surgery. The onset time and segmental spread of analgesia, tested by pinprick and thermal pain, seemed to be slightly shorter with etidocaine (not statistically significant). The duration of analgesia and the blood-pressure changes were comparable with both drugs. The incidence and intensity of motor block were higher with etidocaine.", "contents": "Comparison between bupivacaine and etidocaine in lumbar epidural block for urological surgery. Lumbar epidural blocks produced by 17 ml of etidocaine 1% or bupivacaine 0.5%, both with adrenaline (5 mug/ml), have been compared in a double-blind trial in 40 patients, all scheduled for elective urological surgery. The onset time and segmental spread of analgesia, tested by pinprick and thermal pain, seemed to be slightly shorter with etidocaine (not statistically significant). The duration of analgesia and the blood-pressure changes were comparable with both drugs. The incidence and intensity of motor block were higher with etidocaine."}
{"id": "PMID:1101615", "title": "Thoracic epidural analgesia -- a double blind study between etidocaine and bupivacaine.", "content": "In a double-blind study on 40 patients for elective upper abdominal surgery, measurements was made of onset time, duration and segmental spread of analgesia, systolic blood pressure, heart rate and peak expiratory flow during thoracic epidural analgesia with etidocaine 1% and bupivacaine 0.5%, both containing adrenaline 5 mug/ml. No major differences were found between the two agents. The results differ in many aspects from those obtained with these agents in lumbar epidural analgesia.", "contents": "Thoracic epidural analgesia -- a double blind study between etidocaine and bupivacaine. In a double-blind study on 40 patients for elective upper abdominal surgery, measurements was made of onset time, duration and segmental spread of analgesia, systolic blood pressure, heart rate and peak expiratory flow during thoracic epidural analgesia with etidocaine 1% and bupivacaine 0.5%, both containing adrenaline 5 mug/ml. No major differences were found between the two agents. The results differ in many aspects from those obtained with these agents in lumbar epidural analgesia."}
{"id": "PMID:1101616", "title": "Relief of pain following upper abdominal operations by thoracic epidural block with etidocaine.", "content": "Bupivacaine and etidocaine were compared in 0.375% and 0.5% solutions (without adrenaline) in a double-blind study in thoracic epidural analgesia following upper abdominal surgery. Special regard was taken to duration and adequacy of analgesia and changes in motor function. Duration of analgesia was roughly comparable for all four solutions. Bupivacaine 0.375% and etidocaine 0.5% seemed to be appropriate concentrations for adequate pain relief. Motor function, as assessed by changes in FVC, FEV1 and PEFR was not influenced to any greater extent. A progressive fall in FVC with successive injections, indicating increasing motor weakness, did not occur.", "contents": "Relief of pain following upper abdominal operations by thoracic epidural block with etidocaine. Bupivacaine and etidocaine were compared in 0.375% and 0.5% solutions (without adrenaline) in a double-blind study in thoracic epidural analgesia following upper abdominal surgery. Special regard was taken to duration and adequacy of analgesia and changes in motor function. Duration of analgesia was roughly comparable for all four solutions. Bupivacaine 0.375% and etidocaine 0.5% seemed to be appropriate concentrations for adequate pain relief. Motor function, as assessed by changes in FVC, FEV1 and PEFR was not influenced to any greater extent. A progressive fall in FVC with successive injections, indicating increasing motor weakness, did not occur."}
{"id": "PMID:1101618", "title": "A double-blind trial of bupivacaine and etidocaine in epidural block for surgical induction of labour.", "content": "Compared to bupivacaine, etidocaine has a quicker onset of action both in terms of initial analgesia and time taken to reach maximal extent of analgesia. ;owever, its duration of action is significantly shorter and it produces a striking degree of motor loss. These last two properties make etidocaine relatively unsuitable for obstetric practice, where a long duration of action and a reasonable mobility are desirable. However, it has been found useful for forceps delivery, because of the good muscle relaxation obtained and, because of its greater penetration, in abolishing the missed segments sometimes seen with bupivacaine.", "contents": "A double-blind trial of bupivacaine and etidocaine in epidural block for surgical induction of labour. Compared to bupivacaine, etidocaine has a quicker onset of action both in terms of initial analgesia and time taken to reach maximal extent of analgesia. ;owever, its duration of action is significantly shorter and it produces a striking degree of motor loss. These last two properties make etidocaine relatively unsuitable for obstetric practice, where a long duration of action and a reasonable mobility are desirable. However, it has been found useful for forceps delivery, because of the good muscle relaxation obtained and, because of its greater penetration, in abolishing the missed segments sometimes seen with bupivacaine."}
{"id": "PMID:1101619", "title": "A double-blind comparison of single doses of etidocaine 1 % and bupivacaine 0.5 % during continuous lumbar epidural block in obstetrics.", "content": "A double-blind investigation of some of the effects of etidocaine 1 % and bupivacaine 0.5 % was undertaken in patients in established labour. The results showed that the duration of bupivacaine was significantly longer than that of etidocaine, while the number of patients with good analgesia was equal in both groups. Motor blockade was less obvious in those patients receiving bupivacaine, but in neither group was this a finding of importance. At 5 minutes the Apgar scores of neonates from both groups were similar and any differences seen at 1 minute may be attributable to factors other than the drugs on trial.", "contents": "A double-blind comparison of single doses of etidocaine 1 % and bupivacaine 0.5 % during continuous lumbar epidural block in obstetrics. A double-blind investigation of some of the effects of etidocaine 1 % and bupivacaine 0.5 % was undertaken in patients in established labour. The results showed that the duration of bupivacaine was significantly longer than that of etidocaine, while the number of patients with good analgesia was equal in both groups. Motor blockade was less obvious in those patients receiving bupivacaine, but in neither group was this a finding of importance. At 5 minutes the Apgar scores of neonates from both groups were similar and any differences seen at 1 minute may be attributable to factors other than the drugs on trial."}
{"id": "PMID:1101621", "title": "Virus-like particles in Creutzfeldt-Jakob biopsy material.", "content": "In an electron microscopic study of brain biopsy material from 6 patients with Creutzfeldt-Jacob disease, virus-like particles resembling herpes have been seen. These particles were found only in reactive astrocytes. In 2 out of 6 Creutzfeldt-Jacob brain biopsies some profiles were filled with tubular structures resembling the nucleocapsid of paramyxovirus. The findings are compared with other neurological conditions.", "contents": "Virus-like particles in Creutzfeldt-Jakob biopsy material. In an electron microscopic study of brain biopsy material from 6 patients with Creutzfeldt-Jacob disease, virus-like particles resembling herpes have been seen. These particles were found only in reactive astrocytes. In 2 out of 6 Creutzfeldt-Jacob brain biopsies some profiles were filled with tubular structures resembling the nucleocapsid of paramyxovirus. The findings are compared with other neurological conditions."}
{"id": "PMID:1101623", "title": "Tissue culture study of a sacrococcygeal chordoma with further ultrastructural study.", "content": "This report concerns an electron microscopic study of a sacrococcygeal chordoma and its in vitro cultured cells. In vitro, the cells that proliferated in the early phase were predominantly non-vacuolated stellate cells, which were later transformed into vacuolated cells. This suggests that various cell types seen in vivo represent variants of the same cell type at different stages of differentiation and cellular activity. The in vitro tumor cells also show the origin of their vacuoles from both rough endoplasmic reticulum and Golgi membranes. The finding of amorphous and granular material and collagen fibrils in the extracellular spaces of cultured cells seems to suggest that chordoma cells have certain synthetic and secretory activity.", "contents": "Tissue culture study of a sacrococcygeal chordoma with further ultrastructural study. This report concerns an electron microscopic study of a sacrococcygeal chordoma and its in vitro cultured cells. In vitro, the cells that proliferated in the early phase were predominantly non-vacuolated stellate cells, which were later transformed into vacuolated cells. This suggests that various cell types seen in vivo represent variants of the same cell type at different stages of differentiation and cellular activity. The in vitro tumor cells also show the origin of their vacuoles from both rough endoplasmic reticulum and Golgi membranes. The finding of amorphous and granular material and collagen fibrils in the extracellular spaces of cultured cells seems to suggest that chordoma cells have certain synthetic and secretory activity."}
{"id": "PMID:1101624", "title": "Intravitreal injection of vancomycin in experimental staphylococcal endophthalmitis.", "content": "Toxicity, clearance, and therapeutic effectiveness of intravitreal vancomycin hydrochloride injection in experimentally induced staphylococcal endophthalmitis were evaluated. Vancomycin was found to be nontoxic in a single, 1 mg/0.1 ml intravitreal dose. Therapeutic levels of vancomycin were present in the vitreous for over 72 hours and in the aqueous during a period from 6 to 48 hours after injection. Injection of a methicillin-resistant Staphylococcus aureus produced a panophthalmitis in our systemically treated controls, whereas in the rabbits treated by intraocular injection, the course of the infection was significantly altered.", "contents": "Intravitreal injection of vancomycin in experimental staphylococcal endophthalmitis. Toxicity, clearance, and therapeutic effectiveness of intravitreal vancomycin hydrochloride injection in experimentally induced staphylococcal endophthalmitis were evaluated. Vancomycin was found to be nontoxic in a single, 1 mg/0.1 ml intravitreal dose. Therapeutic levels of vancomycin were present in the vitreous for over 72 hours and in the aqueous during a period from 6 to 48 hours after injection. Injection of a methicillin-resistant Staphylococcus aureus produced a panophthalmitis in our systemically treated controls, whereas in the rabbits treated by intraocular injection, the course of the infection was significantly altered."}
{"id": "PMID:1101625", "title": "Histocompatibility matching in poor-prognosis penetrating keratoplasty.", "content": "Rabbits were haplotyped for the major rabbit leukocyte-locus A (RL-A locus), which is analogous to the human leukocyte-locus A (HL-A locus). The rabbits were divided into five groups, and the groups were arranged to provide a controlled experimental setting. Standardized alkali burns were induced in selected groups to produce heavy vascularization of the corneal bed. Groups with completely histocompatible corneal donors for the RL-A locus had a 9 out of 10 success rate when keratoplasty was performed on a vascularized bed. Another group of rabbits mismatched at the RL-A locus with histoincompatible corneal donors were prepared in the same manner and had a success rate of 1 out 9. A probable significant difference (P less than 0.005) using Fischer's exact test was found between the two groups.", "contents": "Histocompatibility matching in poor-prognosis penetrating keratoplasty. Rabbits were haplotyped for the major rabbit leukocyte-locus A (RL-A locus), which is analogous to the human leukocyte-locus A (HL-A locus). The rabbits were divided into five groups, and the groups were arranged to provide a controlled experimental setting. Standardized alkali burns were induced in selected groups to produce heavy vascularization of the corneal bed. Groups with completely histocompatible corneal donors for the RL-A locus had a 9 out of 10 success rate when keratoplasty was performed on a vascularized bed. Another group of rabbits mismatched at the RL-A locus with histoincompatible corneal donors were prepared in the same manner and had a success rate of 1 out 9. A probable significant difference (P less than 0.005) using Fischer's exact test was found between the two groups."}
{"id": "PMID:1101626", "title": "Conjunctival sensitivity in pathological cases, with simultaneous measurement of corneal and lid margin sensitivity.", "content": "A total of 209 pathological eyes each had 17 localities tested for sensitivity (cornea, caruncle, upper and lower lid margins (centrally, medially and laterally), and corresponding localities on the palpebral conjunctiva, and upper and lower halves of the bulbar conjunctiva). Reduced conjunctival sensitivity is seen in pemphigoid (excluding the lid margin) in contact lens wearers, at sites of nerves transected during operation and in rare cases of infectious conjunctivitis. Isolated corneal hypaesthesia is seen in bacterial or fungal keratitis. In herpes, the hypaesthesia extends over the bulbar conjunctiva, in zoster, over wider areas (including the lid margin). The sensitivity is normal in keratoconjunctivitis sicca and chronic conjunctivitis. In neurological diseases the hyposensitivity could include the cornea, conjunctiva and lid margin. The conclusion is drawn that a study of the conjunctivo-corneal sensitivity can give differential diagnostic information, provided the normal sensitivity range is known. This has been set out in a Table in 10-year age groups.", "contents": "Conjunctival sensitivity in pathological cases, with simultaneous measurement of corneal and lid margin sensitivity. A total of 209 pathological eyes each had 17 localities tested for sensitivity (cornea, caruncle, upper and lower lid margins (centrally, medially and laterally), and corresponding localities on the palpebral conjunctiva, and upper and lower halves of the bulbar conjunctiva). Reduced conjunctival sensitivity is seen in pemphigoid (excluding the lid margin) in contact lens wearers, at sites of nerves transected during operation and in rare cases of infectious conjunctivitis. Isolated corneal hypaesthesia is seen in bacterial or fungal keratitis. In herpes, the hypaesthesia extends over the bulbar conjunctiva, in zoster, over wider areas (including the lid margin). The sensitivity is normal in keratoconjunctivitis sicca and chronic conjunctivitis. In neurological diseases the hyposensitivity could include the cornea, conjunctiva and lid margin. The conclusion is drawn that a study of the conjunctivo-corneal sensitivity can give differential diagnostic information, provided the normal sensitivity range is known. This has been set out in a Table in 10-year age groups."}
{"id": "PMID:1101627", "title": "The pattern of new bone formation in isografts of bone.", "content": "The pattern of new bone formation has been studied in isografts of fresh iliac bone and also in isografts of dead or irradiated iliac bone. Two phases of osteogenesis have been found in some fresh grafts. An early phase occurs during the first 3 weeks after transplantation, whilst the second phase is found after 8 weeks. The first phase is absent from non-viable grafts and it is therefore concluded that cells of the grafts are largely responsible for this early osteogenesis. The second phase, it is suggested, has a major host component, and may be due to induction of osteogenic potential in host mesenchymal cells. There may be some dependence of the second phase on the first but the extent is not clear. Removal of the bone marrow from the graft has little effect upon the first phase of new bone formation and it is suggested that surviving endosteal cells are the main participants in early osteogenesis.", "contents": "The pattern of new bone formation in isografts of bone. The pattern of new bone formation has been studied in isografts of fresh iliac bone and also in isografts of dead or irradiated iliac bone. Two phases of osteogenesis have been found in some fresh grafts. An early phase occurs during the first 3 weeks after transplantation, whilst the second phase is found after 8 weeks. The first phase is absent from non-viable grafts and it is therefore concluded that cells of the grafts are largely responsible for this early osteogenesis. The second phase, it is suggested, has a major host component, and may be due to induction of osteogenic potential in host mesenchymal cells. There may be some dependence of the second phase on the first but the extent is not clear. Removal of the bone marrow from the graft has little effect upon the first phase of new bone formation and it is suggested that surviving endosteal cells are the main participants in early osteogenesis."}
{"id": "PMID:1101628", "title": "Strontium-85 profile counting in fractures of the tibial shaft.", "content": "A total of 203 profile countings were performed on 68 fractures of the tibial shaft, which included 25 normally healing cases, 37 cases of delayed union, and six cases of non-union. The measurements were performed on the 1st and the 7th day after the injection of strontium. In 27 cases the fracture received 2-3 strontium injections with associated profile counting at different stages of the healing process. The cases of normally healing fractures and delayed union showed differences in the average strontium uptake. However, these differences were not distinct enough to enable classification of individual fractures into groups. In cases requiring bone grafting, the transplant was found to accelerate metabolism and increase the accretion of strontium at the site of the fracture. The investigation shows that the real value of strontium profile counting lies in its ability to give information about the osteogenetic activity of the bone, which is a necessary prerequisite for the healing of the fracture.", "contents": "Strontium-85 profile counting in fractures of the tibial shaft. A total of 203 profile countings were performed on 68 fractures of the tibial shaft, which included 25 normally healing cases, 37 cases of delayed union, and six cases of non-union. The measurements were performed on the 1st and the 7th day after the injection of strontium. In 27 cases the fracture received 2-3 strontium injections with associated profile counting at different stages of the healing process. The cases of normally healing fractures and delayed union showed differences in the average strontium uptake. However, these differences were not distinct enough to enable classification of individual fractures into groups. In cases requiring bone grafting, the transplant was found to accelerate metabolism and increase the accretion of strontium at the site of the fracture. The investigation shows that the real value of strontium profile counting lies in its ability to give information about the osteogenetic activity of the bone, which is a necessary prerequisite for the healing of the fracture."}
{"id": "PMID:1101629", "title": "A novel titanium rabbit ear chamber for vital microscopy of bone tissue.", "content": "Seventy-eight small titanium-glass culture chambers of own design for vital microscopy of bone tissue were inserted in the ears in 41 rabbits. After insertion without removing the cartilage on the inner side of ears not previously operated upon, 44 out of 56 chambers (79 per cent) became vascularized. It proved satisfactory for bright field, fluorescence and polarized light microscopical observations of living bone tissue for half a year or longer.", "contents": "A novel titanium rabbit ear chamber for vital microscopy of bone tissue. Seventy-eight small titanium-glass culture chambers of own design for vital microscopy of bone tissue were inserted in the ears in 41 rabbits. After insertion without removing the cartilage on the inner side of ears not previously operated upon, 44 out of 56 chambers (79 per cent) became vascularized. It proved satisfactory for bright field, fluorescence and polarized light microscopical observations of living bone tissue for half a year or longer."}
{"id": "PMID:1101631", "title": "Osteogenesis in rabbit ear chambers.", "content": "Heterotopic osteogenesis was studied in vivo by grafting 246 bone tissue grafts onto 39 cultures chambers inserted in the ears of 27 rabbits. Bone formation was obtained from 23 out of 24 autografts placed inside the optical part (greater than 0.1 mm deep) of 8 ear chambers. No bone tissue formation was observed in the optical part (less than 0.1 mm deep) of 23 chambers in which a total of 129 autografts had been placed in a communicating deeper non-optical part. Bone formation was always obtained after autografting bone tissue to the non-optical part of a titanium-glass ear chamber of own design, and bone tissue entered the optical part of these chambers when this was deeper than 0.1 mm. The possible source of bone formation in rabbit ear chambers is mentioned. Resorption of bone tissue autografts was regularly obtained.", "contents": "Osteogenesis in rabbit ear chambers. Heterotopic osteogenesis was studied in vivo by grafting 246 bone tissue grafts onto 39 cultures chambers inserted in the ears of 27 rabbits. Bone formation was obtained from 23 out of 24 autografts placed inside the optical part (greater than 0.1 mm deep) of 8 ear chambers. No bone tissue formation was observed in the optical part (less than 0.1 mm deep) of 23 chambers in which a total of 129 autografts had been placed in a communicating deeper non-optical part. Bone formation was always obtained after autografting bone tissue to the non-optical part of a titanium-glass ear chamber of own design, and bone tissue entered the optical part of these chambers when this was deeper than 0.1 mm. The possible source of bone formation in rabbit ear chambers is mentioned. Resorption of bone tissue autografts was regularly obtained."}
{"id": "PMID:1101632", "title": "Bone remodelling in rabbit ear chambers. A vital microscopical and histological study.", "content": "Heterotopic autologous bone tissue was studied by vital microscopy in rabbit ear chambers and correlated with the findings from histological sections prepared from the same tissue. The in vivo morphology and remodelling of different bone types were thereby characterized. Autografts were resorbed in a disordered fashion by osteoclasts and woven-fibred bone tissue formed on such grafts. Later parallel-fibred bone, bounded by a seam of osteoid tissue, formed on this scaffold. Bone flakes growing underneath the cover glass were remodelled into shallow hemi-ossicles containing fat marrow, which were always oriented towards the cover glass. Following en bloc removal of the central part of the bone culture, woven-fibred bone rapidly formed within the new connective tissue, filling in the empty space. Such bone was remodelled into cancellous bone containing fat marrow. A balance between resorption and formation was regularly established in the chambers. The bone cultures were thus useful for vital microscopical studies for half a year or longer. In the present study bone remodelling in rabbit ear chambers closely resembled that of orthotopics bone. Ear chamber bone cultures are therefore a versatile in vivo biological model for dynamic bone tissue studies at both tissue and cellular levels.", "contents": "Bone remodelling in rabbit ear chambers. A vital microscopical and histological study. Heterotopic autologous bone tissue was studied by vital microscopy in rabbit ear chambers and correlated with the findings from histological sections prepared from the same tissue. The in vivo morphology and remodelling of different bone types were thereby characterized. Autografts were resorbed in a disordered fashion by osteoclasts and woven-fibred bone tissue formed on such grafts. Later parallel-fibred bone, bounded by a seam of osteoid tissue, formed on this scaffold. Bone flakes growing underneath the cover glass were remodelled into shallow hemi-ossicles containing fat marrow, which were always oriented towards the cover glass. Following en bloc removal of the central part of the bone culture, woven-fibred bone rapidly formed within the new connective tissue, filling in the empty space. Such bone was remodelled into cancellous bone containing fat marrow. A balance between resorption and formation was regularly established in the chambers. The bone cultures were thus useful for vital microscopical studies for half a year or longer. In the present study bone remodelling in rabbit ear chambers closely resembled that of orthotopics bone. Ear chamber bone cultures are therefore a versatile in vivo biological model for dynamic bone tissue studies at both tissue and cellular levels."}
{"id": "PMID:1101633", "title": "Effect of indomethacin on bone remodelling in rabbit ear chambers.", "content": "The effect of indomethacin medication on bone tissue resorption and formation was assessed by qualitative and quantitative microscopy in vivo in 18 rabbit ear chambers in 12 rabbits. Five bone tissue experiments were carried out during half a year using the rabbits as their own controls. One daily oral indomethacin dosage of 10 mg/kg body weight given for 3 weeks inhibited resorption of bone autografts in this type of vital chamber and thus inhibited disordered osteoclastic activity. The effect of indomethacin on ordered osteoclastic activity was not quantitated, nor was its effect on parallel-fibred bone tissue formation assessed. The same dosage of indomethacin clearly retarded woven-fibred bone tissue formation (by 1 week) as compared to control experiments, in which the chambers were used as their own controls. It is concluded that indomethacin treatment of patients liable to develop para-articular ossifications therefore seems rational. Indomethacin and drug-induced arthropathy of the hip-joint is discussed in relation to the presented results. A final appraisal of the role of indomethacin in this complex process must await additional material.", "contents": "Effect of indomethacin on bone remodelling in rabbit ear chambers. The effect of indomethacin medication on bone tissue resorption and formation was assessed by qualitative and quantitative microscopy in vivo in 18 rabbit ear chambers in 12 rabbits. Five bone tissue experiments were carried out during half a year using the rabbits as their own controls. One daily oral indomethacin dosage of 10 mg/kg body weight given for 3 weeks inhibited resorption of bone autografts in this type of vital chamber and thus inhibited disordered osteoclastic activity. The effect of indomethacin on ordered osteoclastic activity was not quantitated, nor was its effect on parallel-fibred bone tissue formation assessed. The same dosage of indomethacin clearly retarded woven-fibred bone tissue formation (by 1 week) as compared to control experiments, in which the chambers were used as their own controls. It is concluded that indomethacin treatment of patients liable to develop para-articular ossifications therefore seems rational. Indomethacin and drug-induced arthropathy of the hip-joint is discussed in relation to the presented results. A final appraisal of the role of indomethacin in this complex process must await additional material."}
{"id": "PMID:1101634", "title": "An interactive program for the analysis of ENG tracings.", "content": "An interactive program for clinical and research evaluation of vestibular nystagmus has been developed. Because of the interactive structure of the program, the clinician can make immediate decisions on the interpretation of controversial aspects of the nystagmogram; therefore even the more irregular and noisy ENG tracings, frequently obtained from pathological subjects, can be easily processed. In this way, speed and accuracy of computer processing are coupled with intelligent activity and critical judgement of the clinical evaluation. The program can be implemented on a general purpose minicomputer and no specific technical training is required for its use. The structure of the program and the operations performed by main subsystems are discussed in detail. Examples of ENG tracings processed with the program are reported.", "contents": "An interactive program for the analysis of ENG tracings. An interactive program for clinical and research evaluation of vestibular nystagmus has been developed. Because of the interactive structure of the program, the clinician can make immediate decisions on the interpretation of controversial aspects of the nystagmogram; therefore even the more irregular and noisy ENG tracings, frequently obtained from pathological subjects, can be easily processed. In this way, speed and accuracy of computer processing are coupled with intelligent activity and critical judgement of the clinical evaluation. The program can be implemented on a general purpose minicomputer and no specific technical training is required for its use. The structure of the program and the operations performed by main subsystems are discussed in detail. Examples of ENG tracings processed with the program are reported."}
{"id": "PMID:1101641", "title": "III. Evaluation of the forward triangle area in dye curves from infants and children studied by earpiece densitometry.", "content": "Onehundred and six dye curves recorded by earpiece densitometry in shunt-free children were analysed with respect to the spatial relationships between the forward triangle and the area under the total primary dye curve. It is suggested that the relationship between these two areas, the forward triangle factor, should be 0.32 for studies in children with the use of dichromatic earpiece densitometry.", "contents": "III. Evaluation of the forward triangle area in dye curves from infants and children studied by earpiece densitometry. Onehundred and six dye curves recorded by earpiece densitometry in shunt-free children were analysed with respect to the spatial relationships between the forward triangle and the area under the total primary dye curve. It is suggested that the relationship between these two areas, the forward triangle factor, should be 0.32 for studies in children with the use of dichromatic earpiece densitometry."}
{"id": "PMID:1101635", "title": "Experimental studies on the treatment of unilateral vocal cord paralysis.", "content": "After the injection of Teflon and Silicon behind the vocal cords of sheeps we noticed that these materials are quite well supported. The little particles of Teflon are surrounded by conjective tissue wheras the Silicon is enclosed in an unic membrane. We could not see a migration of the materials in the depth.", "contents": "Experimental studies on the treatment of unilateral vocal cord paralysis. After the injection of Teflon and Silicon behind the vocal cords of sheeps we noticed that these materials are quite well supported. The little particles of Teflon are surrounded by conjective tissue wheras the Silicon is enclosed in an unic membrane. We could not see a migration of the materials in the depth."}
{"id": "PMID:1101642", "title": "IV. Evaluation of dichromatic earpiece densitometry in children with left-to-right shunts.", "content": "Earpiece densitometry was performed in 60 infants and children with left-to-right cardiovascular shunts. The method was found to possess a high diagnostic sensitivity with respect to identification of the left-to-right shunt. Earpiece densitometry was in this respect clearly superior to conventional method using oxygen saturation figures. Quantitation of pulmonary blood flow by both densitiometric and oxygen content technique in a small number of patients indicates that the dye dilution technique gives values somewhat in excess of the Fick method. It is concluded that quantitation of the magnitude of the left-to-right shunt and pulmonary blood flow is disturbed by the fact that indicator material is recirculated before the ascending limb of the primary curve is fully inscribed.", "contents": "IV. Evaluation of dichromatic earpiece densitometry in children with left-to-right shunts. Earpiece densitometry was performed in 60 infants and children with left-to-right cardiovascular shunts. The method was found to possess a high diagnostic sensitivity with respect to identification of the left-to-right shunt. Earpiece densitometry was in this respect clearly superior to conventional method using oxygen saturation figures. Quantitation of pulmonary blood flow by both densitiometric and oxygen content technique in a small number of patients indicates that the dye dilution technique gives values somewhat in excess of the Fick method. It is concluded that quantitation of the magnitude of the left-to-right shunt and pulmonary blood flow is disturbed by the fact that indicator material is recirculated before the ascending limb of the primary curve is fully inscribed."}
{"id": "PMID:1101636", "title": "Planar relationships of the semicircular canals in man.", "content": "Principal-component analyses were determined on a series of points measured from the dissected bony labyrinth of ten human skulls, resulting in planar equations for each of the six semicircular canals. Following this, angles were calculated between the ipsilateral canal planes, between opposite synergistically acting canal planes and between each canal and the Reid stereotaxic planes. Results indicated that pairs of ipsilateral canals were nearly perpendicular, with the excpetion of the angle formed between the anterior and horizontal canal (mean = 111 degrees). Pairs of contralateral synergistic canal planes formed angles of 19 degrees between right and left horizontal canal planes and 23-24 degrees between vertical canal pairs. The horizontal canals formed an angle of 25 degrees with the Reid horizontal plane. Mathematical equations of the semicircular canals were used to predict the optimal head position for rotational and caloric stimulation.", "contents": "Planar relationships of the semicircular canals in man. Principal-component analyses were determined on a series of points measured from the dissected bony labyrinth of ten human skulls, resulting in planar equations for each of the six semicircular canals. Following this, angles were calculated between the ipsilateral canal planes, between opposite synergistically acting canal planes and between each canal and the Reid stereotaxic planes. Results indicated that pairs of ipsilateral canals were nearly perpendicular, with the excpetion of the angle formed between the anterior and horizontal canal (mean = 111 degrees). Pairs of contralateral synergistic canal planes formed angles of 19 degrees between right and left horizontal canal planes and 23-24 degrees between vertical canal pairs. The horizontal canals formed an angle of 25 degrees with the Reid horizontal plane. Mathematical equations of the semicircular canals were used to predict the optimal head position for rotational and caloric stimulation."}
{"id": "PMID:1101637", "title": "The orientation of the semicircular canals in the guinea pig.", "content": "In 10 adult guinea pigs the stereotaxic coordinates of a series of points along each osseous semicircular canal were analysed to yield an equation of that canal plane in stereotaxic space. Angular relationships among the canal planes and between the canal planes and the major stereotaxic planes are presented together with the optimal positions of the head for physiological stimulation of each canal or pair of synergistic canals. The planes of the semicircular canals in each labyrinth are not perpendicular to one another and the planes of contralateral synergistic canals depart from parallelism by about 30 degrees.", "contents": "The orientation of the semicircular canals in the guinea pig. In 10 adult guinea pigs the stereotaxic coordinates of a series of points along each osseous semicircular canal were analysed to yield an equation of that canal plane in stereotaxic space. Angular relationships among the canal planes and between the canal planes and the major stereotaxic planes are presented together with the optimal positions of the head for physiological stimulation of each canal or pair of synergistic canals. The planes of the semicircular canals in each labyrinth are not perpendicular to one another and the planes of contralateral synergistic canals depart from parallelism by about 30 degrees."}
{"id": "PMID:1101643", "title": "Reticulosarcomatosis originating from skin--A clinicopathological study.", "content": "The case is that of a 65 year-old male with reticulosarcomatosis originating from the skin. There were multiple tumors composed of reticulum cells located only in the skin, particularly around the large joints. The entire course of illness was three years and five months, during which period regression by irradiation, recurrence, or new formation of tumors were repeatedly seen. At the end systemic tumor spread not only of the skin but also in various visceral organs including lymph nodes occurred. From histological and electron microscopic examinations repeatedly performed with biopsy materials, and finally with autopsy materials it was ascertained that the tumor cells had become smaller in size with less lymphoid cell infiltration at the terminal stage.", "contents": "Reticulosarcomatosis originating from skin--A clinicopathological study. The case is that of a 65 year-old male with reticulosarcomatosis originating from the skin. There were multiple tumors composed of reticulum cells located only in the skin, particularly around the large joints. The entire course of illness was three years and five months, during which period regression by irradiation, recurrence, or new formation of tumors were repeatedly seen. At the end systemic tumor spread not only of the skin but also in various visceral organs including lymph nodes occurred. From histological and electron microscopic examinations repeatedly performed with biopsy materials, and finally with autopsy materials it was ascertained that the tumor cells had become smaller in size with less lymphoid cell infiltration at the terminal stage."}
{"id": "PMID:1101638", "title": "Survival after haemorrhage from the brachiocephalic truncus following tracheostomy.", "content": "A patient with two massive haemorrhages from the brachiocephalic truncus after tracheostomy is reported. An analysis of 6 previously reported long-term survivors following this complication shows that the initial care in controlling the haemorrhage is of major importance. The operation method of choice is permanent division of the brachiocephalic truncus. Transitory or no neurological postoperative complications were found.", "contents": "Survival after haemorrhage from the brachiocephalic truncus following tracheostomy. A patient with two massive haemorrhages from the brachiocephalic truncus after tracheostomy is reported. An analysis of 6 previously reported long-term survivors following this complication shows that the initial care in controlling the haemorrhage is of major importance. The operation method of choice is permanent division of the brachiocephalic truncus. Transitory or no neurological postoperative complications were found."}
{"id": "PMID:1101644", "title": "The effect of cyclophosphamide, methotrexate and X-irradiation on the ultrastructure and endocytic capacity of murine peritoneal macrophages.", "content": "100 mg/kg cyclophosphamide given subcutaneously daily for 5 days and whole-body X-irradiation (500 r) had an appreciable effect on the phagocytosis of E. coli by mouse peritoneal macrophages. Less bacteria were ingested by cells from the treated mice as compared with control cells. In contrast, whole-body X-irradiation had a stimulatory effect on pinocytosis as compared with cells from cyclophosphamide treated mice or control mice. Morphological changes in the cells from animals treated with cyclophosphamide or X-rays were evident. The cells were larger than normal macrophages and contained large amounts of lipid and phagolysosomes and had more vacuoles as compared with control cells. Many giant cells were present in the treated mice. Treatment of mice with methotrexate for 5 days, although affecting the cells morphologically in that they were larger than normal cells and had many vacuoles did not appear to have any effect on either phagocytosis or pinocytosis. Cells from methotrexate treated animals did not contain the large quantities of lipid seen in cells from the cyclophosphamide treated and X-irradiated mice.", "contents": "The effect of cyclophosphamide, methotrexate and X-irradiation on the ultrastructure and endocytic capacity of murine peritoneal macrophages. 100 mg/kg cyclophosphamide given subcutaneously daily for 5 days and whole-body X-irradiation (500 r) had an appreciable effect on the phagocytosis of E. coli by mouse peritoneal macrophages. Less bacteria were ingested by cells from the treated mice as compared with control cells. In contrast, whole-body X-irradiation had a stimulatory effect on pinocytosis as compared with cells from cyclophosphamide treated mice or control mice. Morphological changes in the cells from animals treated with cyclophosphamide or X-rays were evident. The cells were larger than normal macrophages and contained large amounts of lipid and phagolysosomes and had more vacuoles as compared with control cells. Many giant cells were present in the treated mice. Treatment of mice with methotrexate for 5 days, although affecting the cells morphologically in that they were larger than normal cells and had many vacuoles did not appear to have any effect on either phagocytosis or pinocytosis. Cells from methotrexate treated animals did not contain the large quantities of lipid seen in cells from the cyclophosphamide treated and X-irradiated mice."}
{"id": "PMID:1101645", "title": "Experimental evaluation of the velvet pad rinse technique as a microbiological sampling method.", "content": "The use of velvet pads glued to aluminium foil has been evaluated as a microbiological sampling method in quantitative bacterial recovery experiments, and the efficiency and precision have been determined. The velvet pads uptake of Staphylococcus epidermidis was 0.93 (or 93 per cent) after sampling from a test surface on blood agar, while the release by subsequent imprinting on blood agar was 0.02, the median effective transfer being 0.02. A mechanical rinse and shake procedure of the velvet pad in 0.9 per cent saline followed by centrifugation and surface plating significantly increased the median release to 0.66, the median effective transfer being 0.61. There was no difference in uptake, release and effective transfer between a pure culture of S. epidermidis and a mixed culture of S. epiderimidis and Escherichia coli. Storage of the velvet pad in 0.9 per cent saline for 2 h at room temperature did not influence bacterial recovery significantly, in contrast to a significant decrease after storage in saline for 24 h or storage in a dry Petri dish for 2 h. The high and fairly constant efficiency of bacterial recovery of the velvet pad rinse technique suggests that it could be employed clinically.", "contents": "Experimental evaluation of the velvet pad rinse technique as a microbiological sampling method. The use of velvet pads glued to aluminium foil has been evaluated as a microbiological sampling method in quantitative bacterial recovery experiments, and the efficiency and precision have been determined. The velvet pads uptake of Staphylococcus epidermidis was 0.93 (or 93 per cent) after sampling from a test surface on blood agar, while the release by subsequent imprinting on blood agar was 0.02, the median effective transfer being 0.02. A mechanical rinse and shake procedure of the velvet pad in 0.9 per cent saline followed by centrifugation and surface plating significantly increased the median release to 0.66, the median effective transfer being 0.61. There was no difference in uptake, release and effective transfer between a pure culture of S. epidermidis and a mixed culture of S. epiderimidis and Escherichia coli. Storage of the velvet pad in 0.9 per cent saline for 2 h at room temperature did not influence bacterial recovery significantly, in contrast to a significant decrease after storage in saline for 24 h or storage in a dry Petri dish for 2 h. The high and fairly constant efficiency of bacterial recovery of the velvet pad rinse technique suggests that it could be employed clinically."}
{"id": "PMID:1101646", "title": "Arterial pressure, cardiac output and systemic resistance before and after pithing in normotensive and spontaneously hypertensive rats.", "content": "After complete cardiovascular denervation mean arterial pressure (MAP) falls to almost equally low levels in spontaneously hypertensive rats (SHR) and normotensive control rats (NCR). This has earlier been suggested to indicate a dominance of neurogenic mechanisms in established SHR hypertension. -- In the present study, total peripheral resistance (TPR) remains, however, some 35 per cent higher in adult SHR than in NCR after pithing while cardiac output (CO), and stroke volume, is 35 per cent lower in SHR. These opposite differences in TPR and CO after denervation, resulting in equal MAP levels in SHR and NCR, seem rather to be a consequence of the rapidly established structural adaptation that affects all SHR high-pressure cardiovascular sections. Thus, the SHR precapillary resistance vessels display thickened walls and luminal narrowing, which keeps TPR higher than in NCR even during maximal vasodilatation. Due to hypertrophy, the SHR left ventricle exhibits a reduced myocardial stretch for a given filling pressure and stroke volume is consequently reduced more than in NCR after complete denervation. -- Paradoxically, therefore, rather than reflecting any dominance of neurogenic mechanisms in established SHR hypertension the MAP equalization in SHR and NCR after cardiovascular denervation emphasizes the hemodynamic importance of cardiovascular structural changes present in hypertension.", "contents": "Arterial pressure, cardiac output and systemic resistance before and after pithing in normotensive and spontaneously hypertensive rats. After complete cardiovascular denervation mean arterial pressure (MAP) falls to almost equally low levels in spontaneously hypertensive rats (SHR) and normotensive control rats (NCR). This has earlier been suggested to indicate a dominance of neurogenic mechanisms in established SHR hypertension. -- In the present study, total peripheral resistance (TPR) remains, however, some 35 per cent higher in adult SHR than in NCR after pithing while cardiac output (CO), and stroke volume, is 35 per cent lower in SHR. These opposite differences in TPR and CO after denervation, resulting in equal MAP levels in SHR and NCR, seem rather to be a consequence of the rapidly established structural adaptation that affects all SHR high-pressure cardiovascular sections. Thus, the SHR precapillary resistance vessels display thickened walls and luminal narrowing, which keeps TPR higher than in NCR even during maximal vasodilatation. Due to hypertrophy, the SHR left ventricle exhibits a reduced myocardial stretch for a given filling pressure and stroke volume is consequently reduced more than in NCR after complete denervation. -- Paradoxically, therefore, rather than reflecting any dominance of neurogenic mechanisms in established SHR hypertension the MAP equalization in SHR and NCR after cardiovascular denervation emphasizes the hemodynamic importance of cardiovascular structural changes present in hypertension."}
{"id": "PMID:1101647", "title": "Synaptosomes containing large agranular vesicles isolated from developing rat brain.", "content": "Using the subcellular fractionation technique the fine structure of the isolated nerve endings (synaptosomes) from the hemispheres and brain stem of the 1-day old and adult rat was examined. In the synaptosomal fractions of the brain of 1-day old rats we observed a new type of nerve endings containing predominantly large agranular vesicles about 1,000 A in diameter. After incubation with alpha-methylnoradrenaline or 5-hydroxydopamine these vesicles remained agranular. It is assumed that the new type of large vesicles represent developing synaptic vesicles.", "contents": "Synaptosomes containing large agranular vesicles isolated from developing rat brain. Using the subcellular fractionation technique the fine structure of the isolated nerve endings (synaptosomes) from the hemispheres and brain stem of the 1-day old and adult rat was examined. In the synaptosomal fractions of the brain of 1-day old rats we observed a new type of nerve endings containing predominantly large agranular vesicles about 1,000 A in diameter. After incubation with alpha-methylnoradrenaline or 5-hydroxydopamine these vesicles remained agranular. It is assumed that the new type of large vesicles represent developing synaptic vesicles."}
{"id": "PMID:1101649", "title": "Stereotactic exploration of brain tumours by ultrasound.", "content": "A report upon a new technique for investigation of brain tumour anatomy by means of stereotactically directed ultrasound is presented with some illustrative cases. Information obtained with this technique has been found useful when deciding the treatment program for the tumour.", "contents": "Stereotactic exploration of brain tumours by ultrasound. A report upon a new technique for investigation of brain tumour anatomy by means of stereotactically directed ultrasound is presented with some illustrative cases. Information obtained with this technique has been found useful when deciding the treatment program for the tumour."}
{"id": "PMID:1101650", "title": "Computer assisted axial tomography in cerebrovascular lesions.", "content": "Computer assisted axial tomography was performed in 32 patients with clinically possible cerebrovascular lesions. Decreased absorption as a sign of infarction was found in 15, increased absorption as a sign of hemorrhage in 7 cases. In another 7 cases, diagnosed as hemorrhagic infarction, a simultaneous occurrence of increased and decreased absorption appeared. Increased absorption might also depend on compression effect of edema. In three cases no abnormal attenuation could be demonstrated. Infarctions could be observed years after the onset of the symptoms; intracerebral hemorrhages regressed slowly.", "contents": "Computer assisted axial tomography in cerebrovascular lesions. Computer assisted axial tomography was performed in 32 patients with clinically possible cerebrovascular lesions. Decreased absorption as a sign of infarction was found in 15, increased absorption as a sign of hemorrhage in 7 cases. In another 7 cases, diagnosed as hemorrhagic infarction, a simultaneous occurrence of increased and decreased absorption appeared. Increased absorption might also depend on compression effect of edema. In three cases no abnormal attenuation could be demonstrated. Infarctions could be observed years after the onset of the symptoms; intracerebral hemorrhages regressed slowly."}
{"id": "PMID:1101651", "title": "Cross-sectional anatomic images by gamma ray transmission scanning.", "content": "60Co gamma-ray transmission data were measured along linear scan paths at a number of angular orientations with respect to the patient and submitted to a computer software program. Reconstructed images are displayed as digital density printouts and as isodensity contours on an x-y plotter or oscilloscope screen. Image resolution is limited primarily by factors such as collimation and the amount of transmission data collected; with the rather rudimentary apparatus at disposal, a spatial resolution better than 5 mm has been achieved.", "contents": "Cross-sectional anatomic images by gamma ray transmission scanning. 60Co gamma-ray transmission data were measured along linear scan paths at a number of angular orientations with respect to the patient and submitted to a computer software program. Reconstructed images are displayed as digital density printouts and as isodensity contours on an x-y plotter or oscilloscope screen. Image resolution is limited primarily by factors such as collimation and the amount of transmission data collected; with the rather rudimentary apparatus at disposal, a spatial resolution better than 5 mm has been achieved."}
{"id": "PMID:1101654", "title": "Some problems concerning the determination of different corrinoids by the plate method with Escherichia coli 113-3.", "content": "Biological activity of some vitamin B12 forms for Escherichia coli 113-3 and the effect of methionine on the assay of these compounds by E. coli 113-3 were studied. It was found that the coenzymatic form had the highest biological activity and that under experimental conditions methionine was an interfering factor in determination of the coenzymatic form and the methyl derivative of B12 only. Otherwise, metionine did not affect the determination of cyanocobalamin an hydroxycobalamin even when the methionine and vitamin B12 ratio was 32 000 : 1.", "contents": "Some problems concerning the determination of different corrinoids by the plate method with Escherichia coli 113-3. Biological activity of some vitamin B12 forms for Escherichia coli 113-3 and the effect of methionine on the assay of these compounds by E. coli 113-3 were studied. It was found that the coenzymatic form had the highest biological activity and that under experimental conditions methionine was an interfering factor in determination of the coenzymatic form and the methyl derivative of B12 only. Otherwise, metionine did not affect the determination of cyanocobalamin an hydroxycobalamin even when the methionine and vitamin B12 ratio was 32 000 : 1."}
{"id": "PMID:1101655", "title": "Mechanism of assembly of tobacco mosaic virus in vitro.", "content": "We previously proposed that the assembly reaction of TMV in vitro is initiated by 20S protein aggregate specifically interacting to the 5'-end of TMV-RNA, after which the helical rod grows by the addition of protein subunits. Other workers have reported that the source of protein for the growing helix is also the 20S protein aggregate, but not protein subunits. We now summarize the experimental results that confirm our previous hypothesis. 1) TMV-particle, as gauged by infectivity assay and sucrose gradient analysis, could be formed from PRR under conditions where the formation of 20S protein aggregate could not occur. 2) TMV-particle could be formed from PRR by stepwise addition of NBS-modified protein which is lacking the ability to form 20S protein aggregate. 3) The stable disk aggregate of CGMMV-protein are unable to grow the helical rod. 4) The process of rod elongation by protein subunits was observed directly in electron micrography.", "contents": "Mechanism of assembly of tobacco mosaic virus in vitro. We previously proposed that the assembly reaction of TMV in vitro is initiated by 20S protein aggregate specifically interacting to the 5'-end of TMV-RNA, after which the helical rod grows by the addition of protein subunits. Other workers have reported that the source of protein for the growing helix is also the 20S protein aggregate, but not protein subunits. We now summarize the experimental results that confirm our previous hypothesis. 1) TMV-particle, as gauged by infectivity assay and sucrose gradient analysis, could be formed from PRR under conditions where the formation of 20S protein aggregate could not occur. 2) TMV-particle could be formed from PRR by stepwise addition of NBS-modified protein which is lacking the ability to form 20S protein aggregate. 3) The stable disk aggregate of CGMMV-protein are unable to grow the helical rod. 4) The process of rod elongation by protein subunits was observed directly in electron micrography."}
{"id": "PMID:1101658", "title": "DNA repair and evolutionary considerations. A search for a general principle in nuclear biology with use of radiation as a probe.", "content": "Three types of DNA repair are known: photoenzymatic repair, excision repair, and tolerance repair (the ability to generate damage-free copies of DNA, which could be not exact copies, from damaged DNA templates). Photoenzymatic repair involves the most simple molecular mechanisms and is the most specific (effective only for pyrimidine dimers) and the most widely distributed among present living organisms, followed by excision repair and then by tolerance repair in order of increasing complexity. It is proposed that these repair systems also evolved in this order after the primordial life was created by solar UV light. Current but fragmentary evidence concerning the molecular and genetic bases of the three types of repair tends to support the idea that the increase in DNA-content needed for the evolution of higher forms from the prokaryotes may have been accompanied by the inherent threat of fragility of the giant DNA molecules, so that the higher forms now possess a greatly increased tolerance capacity. Probably the increase is approximately proportional to their DNA content compared with that of prokaryotes. It is shown theoretically that photoenzymatic and excision repair alone cannot deal with the inherent fragility of giant DNA. The development of tolerance-repair capacity, however, inevitably enhances probability of errors in the repair of DNA damage, leading to the accumulation of misrepair events such as mutation, chromosomal aberration and cancer. It is proposed that DNA repair and misreplication repair are responsible for preventing living organisms from regressing to the randomness-dominated world of the non-living. Living organisms would have evolved slowly along zigzag lines between error and repair of DNA.", "contents": "DNA repair and evolutionary considerations. A search for a general principle in nuclear biology with use of radiation as a probe. Three types of DNA repair are known: photoenzymatic repair, excision repair, and tolerance repair (the ability to generate damage-free copies of DNA, which could be not exact copies, from damaged DNA templates). Photoenzymatic repair involves the most simple molecular mechanisms and is the most specific (effective only for pyrimidine dimers) and the most widely distributed among present living organisms, followed by excision repair and then by tolerance repair in order of increasing complexity. It is proposed that these repair systems also evolved in this order after the primordial life was created by solar UV light. Current but fragmentary evidence concerning the molecular and genetic bases of the three types of repair tends to support the idea that the increase in DNA-content needed for the evolution of higher forms from the prokaryotes may have been accompanied by the inherent threat of fragility of the giant DNA molecules, so that the higher forms now possess a greatly increased tolerance capacity. Probably the increase is approximately proportional to their DNA content compared with that of prokaryotes. It is shown theoretically that photoenzymatic and excision repair alone cannot deal with the inherent fragility of giant DNA. The development of tolerance-repair capacity, however, inevitably enhances probability of errors in the repair of DNA damage, leading to the accumulation of misrepair events such as mutation, chromosomal aberration and cancer. It is proposed that DNA repair and misreplication repair are responsible for preventing living organisms from regressing to the randomness-dominated world of the non-living. Living organisms would have evolved slowly along zigzag lines between error and repair of DNA."}
{"id": "PMID:1101665", "title": "Pituitary function in chronic alcoholism.", "content": "Elevated basal LH levels and an exaggerated response to LHRH were found in a high proportion of male chronic alcoholics. This was not associated with reduced plasma testosterone values. In addition, previous reports of an absent cortisol response to hypoglycaemia in a proportion of chronic alcoholics are confirmed.", "contents": "Pituitary function in chronic alcoholism. Elevated basal LH levels and an exaggerated response to LHRH were found in a high proportion of male chronic alcoholics. This was not associated with reduced plasma testosterone values. In addition, previous reports of an absent cortisol response to hypoglycaemia in a proportion of chronic alcoholics are confirmed."}
{"id": "PMID:1101670", "title": "Disposition of catecholamine-derived alkaloids in mammalian systems.", "content": "Tetrahydropapaveroline, the tetrahydroisoquinoline alkaloid derived from dopamine, is converted in vivo by rats and by rat liver and brain preparations to tetrahydroprotoberberine alkaloids. The latter alkaloids have also been identified for the first time in the urine of parkinsonian patients receiving L-dopa therapy. These findings suggest that man, like plants, may have the ability to elaborate several classes of alkaloids with potentially important pharmacological consequences. Thus, this newly demonstrated ability of mammalian systems to evoke the biosynthesis of benzyl-tetrahydroisoquinoline-derived alkaloids - a capability previously considered unique to plants - elects the tetrahydroprotoberberine alkaloids as representative of the first class of a possible constellation of complex mammalian alkaloids elaborated from the neuroamines.", "contents": "Disposition of catecholamine-derived alkaloids in mammalian systems. Tetrahydropapaveroline, the tetrahydroisoquinoline alkaloid derived from dopamine, is converted in vivo by rats and by rat liver and brain preparations to tetrahydroprotoberberine alkaloids. The latter alkaloids have also been identified for the first time in the urine of parkinsonian patients receiving L-dopa therapy. These findings suggest that man, like plants, may have the ability to elaborate several classes of alkaloids with potentially important pharmacological consequences. Thus, this newly demonstrated ability of mammalian systems to evoke the biosynthesis of benzyl-tetrahydroisoquinoline-derived alkaloids - a capability previously considered unique to plants - elects the tetrahydroprotoberberine alkaloids as representative of the first class of a possible constellation of complex mammalian alkaloids elaborated from the neuroamines."}
{"id": "PMID:1101672", "title": "[Morphology of the corneal endothelium under normal conditions and during regeneration after mechanical injury].", "content": "On a preparation of the corneal endothelium membrane from a rabbit, the morphological structure of the endothelium is examined under normal physiological conditions and during regeneration after abrasion by mechanical means.  Thematerial which is submitted here is outlined in two chapters: (1) The morphology of the normal corneal endothelium; (a) in the contest of the literature on the subject, (b) in comparison with different methods of preparation. (2) The morphology of endothelium regeneration; (a) in the literature as physiological regeneration and, after experimental abrasion, as a model for human endothelium pathology, (b)as a systematic examination at different points of time after mechanical abrasion and in single phases of indirect and direct cell multiplication. After a survey of the comprehensive literature on the subject, several histological fixations and methodds of endothelium preparation as a membrane are given. In a comparison of the findings of the presentation of the normal endothelium, these methods are described in virw of a specific presentation of pathological endothelium rocesses. On the basis of the dyeing characteristics of the cells after intraocular pre-fixation; the evidence of the viscous mucopolysaccharoid membrane on the cell surface (the membrane having been dissected from the endothelium) is discussed...", "contents": "[Morphology of the corneal endothelium under normal conditions and during regeneration after mechanical injury]. On a preparation of the corneal endothelium membrane from a rabbit, the morphological structure of the endothelium is examined under normal physiological conditions and during regeneration after abrasion by mechanical means.  Thematerial which is submitted here is outlined in two chapters: (1) The morphology of the normal corneal endothelium; (a) in the contest of the literature on the subject, (b) in comparison with different methods of preparation. (2) The morphology of endothelium regeneration; (a) in the literature as physiological regeneration and, after experimental abrasion, as a model for human endothelium pathology, (b)as a systematic examination at different points of time after mechanical abrasion and in single phases of indirect and direct cell multiplication. After a survey of the comprehensive literature on the subject, several histological fixations and methodds of endothelium preparation as a membrane are given. In a comparison of the findings of the presentation of the normal endothelium, these methods are described in virw of a specific presentation of pathological endothelium rocesses. On the basis of the dyeing characteristics of the cells after intraocular pre-fixation; the evidence of the viscous mucopolysaccharoid membrane on the cell surface (the membrane having been dissected from the endothelium) is discussed..."}
{"id": "PMID:1101675", "title": "New observations on the effects of atropine on the sinoatrial and atrioventricular nodes in man.", "content": "Previous observations of slowing of the heart rate after administration of atropine in doses smaller than 0.4 mg and recent reports of development of rhythm disorders in patients with acute myocardial infarction given atropine prompted us to evaluate systematically the effects of various doses of atropine (0.1 to 0.8 mg) on the response of the sinoatrial (S-A) and atrioventricular (A-V) nodes in healthy volunteers. The response of the S-A node to atropine was characteristically bimodal, slowing at smaller doses and accelerating at larger doses. In contrast, the A-V node showed acceleration of conduction in response to all doses of atropine used. A hypothesis based on current understanding of the electrophysiologic parameters governing impulse formation and impulse conduction is advanced to explain the apparent paradox in the S-A and A-V nodal responses to small doses of atropine. The results suggest the need for caution and continuous rhythm monitoring when giving atropine to patients with acute myocardial infarction.", "contents": "New observations on the effects of atropine on the sinoatrial and atrioventricular nodes in man. Previous observations of slowing of the heart rate after administration of atropine in doses smaller than 0.4 mg and recent reports of development of rhythm disorders in patients with acute myocardial infarction given atropine prompted us to evaluate systematically the effects of various doses of atropine (0.1 to 0.8 mg) on the response of the sinoatrial (S-A) and atrioventricular (A-V) nodes in healthy volunteers. The response of the S-A node to atropine was characteristically bimodal, slowing at smaller doses and accelerating at larger doses. In contrast, the A-V node showed acceleration of conduction in response to all doses of atropine used. A hypothesis based on current understanding of the electrophysiologic parameters governing impulse formation and impulse conduction is advanced to explain the apparent paradox in the S-A and A-V nodal responses to small doses of atropine. The results suggest the need for caution and continuous rhythm monitoring when giving atropine to patients with acute myocardial infarction."}
{"id": "PMID:1101677", "title": "Spontaneous abortion as a screening device. The effect of fetal survival on the incidence of birth defects.", "content": "To make sound inferences about the distribution, causes and risks of congenital anomalies requires an understanding of selective survival among fetuses. Studies of spontaneous abortions are therefore advocated. A simple model is proposed of the distribution, among spontaneous abortions and births, of conceptuses with and without anomalies. The resulting equation accomodates both maternal and fetal factors. Interaction between maternal and fetal factors is hypothesized, in the form of a maternal device for screening out anomalous conceptuses. A number of the known facts about congenital anomalies are shown to be plausibly interpreted in terms of the proposed model. Finally, some clinical and epidemiologic implications of the model for the study of spontaneous abortions are pointed out.", "contents": "Spontaneous abortion as a screening device. The effect of fetal survival on the incidence of birth defects. To make sound inferences about the distribution, causes and risks of congenital anomalies requires an understanding of selective survival among fetuses. Studies of spontaneous abortions are therefore advocated. A simple model is proposed of the distribution, among spontaneous abortions and births, of conceptuses with and without anomalies. The resulting equation accomodates both maternal and fetal factors. Interaction between maternal and fetal factors is hypothesized, in the form of a maternal device for screening out anomalous conceptuses. A number of the known facts about congenital anomalies are shown to be plausibly interpreted in terms of the proposed model. Finally, some clinical and epidemiologic implications of the model for the study of spontaneous abortions are pointed out."}
{"id": "PMID:1101678", "title": "Leprosy in Louisiana, 1855-1970. An epidemiologic study of long-term trends.", "content": "Study of a well known focus of leprosy in Louisiana in 1855-1970 showed a decrease in incidence which began before the use of chemotherapy. Many of the cases had onset in a limited area of Louisiana, French Louisiana, where there was a high incidence of multiple family cases. Overall incidence rates were found to be decreasing for each cohort of birth, and within cohorts after 1920, the highest incidence rates were in the age group 10-19. A high incidence rate was found for cases with onset in the years 1900-1929; rather than an epidemic, this was probably related to bias in the recording of year of onset. Although persistent for a long period, the focus remained limited in geographic extent and seems to be disappearing.", "contents": "Leprosy in Louisiana, 1855-1970. An epidemiologic study of long-term trends. Study of a well known focus of leprosy in Louisiana in 1855-1970 showed a decrease in incidence which began before the use of chemotherapy. Many of the cases had onset in a limited area of Louisiana, French Louisiana, where there was a high incidence of multiple family cases. Overall incidence rates were found to be decreasing for each cohort of birth, and within cohorts after 1920, the highest incidence rates were in the age group 10-19. A high incidence rate was found for cases with onset in the years 1900-1929; rather than an epidemic, this was probably related to bias in the recording of year of onset. Although persistent for a long period, the focus remained limited in geographic extent and seems to be disappearing."}
{"id": "PMID:1101680", "title": "Studies of twins with systemic lupus erythematosus. A review of the literature and presentation of 12 additional sets.", "content": "To assess the role of genetic factors in systemic lupus erythematosus (SLE), 12 twon pairs (seven definitely monozygotic, three definitely dizygotic) of which one or both twins had SLE, were studied and compared to 17 twin pairs (12 definitely monozygotic) previously described. In the present series, four of seven (57 per cent) definitely monozygotic pairs were clinically concordant for SLE, satisfying the preliminary criteria of the American Rheumatism Association (ARA). Concordance for the presence of antinuclear factor (ANF) and hypergammaglobulinemia was 71 and tinuclear factor (ANF) and hypergammaglobulinemia was 71 and 87 per cent, respiectively. These data closely agree with those on the 12 definitely monozygotic sets previously described. All three of the dizygotic sets in the present series were discordant for clinical SLE, although one clinically well twin had marked serologic abnormalities. Comparison of these data with thos from other first degree relatives of out twins clearly suggests a strong genetic component in the pathogenesis of SLE. The relative contribution of nongenetic and environmental factors to the expression of the disease is discussed.", "contents": "Studies of twins with systemic lupus erythematosus. A review of the literature and presentation of 12 additional sets. To assess the role of genetic factors in systemic lupus erythematosus (SLE), 12 twon pairs (seven definitely monozygotic, three definitely dizygotic) of which one or both twins had SLE, were studied and compared to 17 twin pairs (12 definitely monozygotic) previously described. In the present series, four of seven (57 per cent) definitely monozygotic pairs were clinically concordant for SLE, satisfying the preliminary criteria of the American Rheumatism Association (ARA). Concordance for the presence of antinuclear factor (ANF) and hypergammaglobulinemia was 71 and tinuclear factor (ANF) and hypergammaglobulinemia was 71 and 87 per cent, respiectively. These data closely agree with those on the 12 definitely monozygotic sets previously described. All three of the dizygotic sets in the present series were discordant for clinical SLE, although one clinically well twin had marked serologic abnormalities. Comparison of these data with thos from other first degree relatives of out twins clearly suggests a strong genetic component in the pathogenesis of SLE. The relative contribution of nongenetic and environmental factors to the expression of the disease is discussed."}
{"id": "PMID:1101681", "title": "Serial complement studies in a patient with Goodpasture's syndrome treated with bilateral nephrectomy and renal transplantation.", "content": "A young male patient with Goodpasture's syndrome was treated with bilateral nephrectomy and when antiglomerular basement membrane antibodies could no longer be detected he received a cadaveric renal homograft. Fifteen months later he is in good health and without signs of pulmonary disease. Renal function is satisfactory, and there are no findings indicating recurrence of the nephritis. Serial complement studies during the entire course revealed varying degrees of activity in the sequence in the different phases: a high degree of in vivo activation of complement was found in the period before the nephrectomy, there was a moderate degree of activation in the period between the nephrectomy and transplantation and, finally, there were no signs of activity in the system after transplantation. This investigation strongly suggests that the complement system is of definite pathogenetic significance in this human equivalent to experimental nephrotoxic nephritis.", "contents": "Serial complement studies in a patient with Goodpasture's syndrome treated with bilateral nephrectomy and renal transplantation. A young male patient with Goodpasture's syndrome was treated with bilateral nephrectomy and when antiglomerular basement membrane antibodies could no longer be detected he received a cadaveric renal homograft. Fifteen months later he is in good health and without signs of pulmonary disease. Renal function is satisfactory, and there are no findings indicating recurrence of the nephritis. Serial complement studies during the entire course revealed varying degrees of activity in the sequence in the different phases: a high degree of in vivo activation of complement was found in the period before the nephrectomy, there was a moderate degree of activation in the period between the nephrectomy and transplantation and, finally, there were no signs of activity in the system after transplantation. This investigation strongly suggests that the complement system is of definite pathogenetic significance in this human equivalent to experimental nephrotoxic nephritis."}
{"id": "PMID:1101682", "title": "Acid phosphatase production as an aid in rapid characterization of Candida species.", "content": "Yeast isolates were tested for phosphatase activity, using p-nitrophenyl phosphate substrate. Several Candida species displayed characteristic patterns. The enzyme activity was absent in all except one of the 78 isolates of Candida albicans tested. C. tropicalis, C. pseudotropicalis, and C. krusei were the most consistent and active enzyme producers. Enzyme production by these yeasts was not influenced by the amount of growth or a decrease in the pH of the culture media. The characteristic enzyme activity of these three yeasts was demonstrated in the presence of concentrations of inorganic phosphate which inhibited the enzyme activity of other Candida species. The activity of isolates of C. gullermondii, C. parapsilosis, and C. stellatoidea was variable, although none of these were strong enzyme producers. It seems feasible to use phosphatase activity as a simple and rapid additional method for the separation of C. albicans from closely related species of the genus.", "contents": "Acid phosphatase production as an aid in rapid characterization of Candida species. Yeast isolates were tested for phosphatase activity, using p-nitrophenyl phosphate substrate. Several Candida species displayed characteristic patterns. The enzyme activity was absent in all except one of the 78 isolates of Candida albicans tested. C. tropicalis, C. pseudotropicalis, and C. krusei were the most consistent and active enzyme producers. Enzyme production by these yeasts was not influenced by the amount of growth or a decrease in the pH of the culture media. The characteristic enzyme activity of these three yeasts was demonstrated in the presence of concentrations of inorganic phosphate which inhibited the enzyme activity of other Candida species. The activity of isolates of C. gullermondii, C. parapsilosis, and C. stellatoidea was variable, although none of these were strong enzyme producers. It seems feasible to use phosphatase activity as a simple and rapid additional method for the separation of C. albicans from closely related species of the genus."}
{"id": "PMID:1101683", "title": "Inadequate cell washing as a source of error in fetal hemoglobin determination.", "content": "Washing the red cells before preparation of the hemolysate is important in avoiding serious clinical errors in fetal hemoglobin determinations. This has been demonstrated using both the alkali denaturation technique and a commercial hemolyzing reagent. Hemolysates prepared from unwashed cells were always above the normal range in samples known to be normal.", "contents": "Inadequate cell washing as a source of error in fetal hemoglobin determination. Washing the red cells before preparation of the hemolysate is important in avoiding serious clinical errors in fetal hemoglobin determinations. This has been demonstrated using both the alkali denaturation technique and a commercial hemolyzing reagent. Hemolysates prepared from unwashed cells were always above the normal range in samples known to be normal."}
{"id": "PMID:1101687", "title": "Pregnancy following renal transplantation.", "content": "From 1968 through 1974 at the University of Minnesota there were 17 pregnancies in 12 patients who were renal transplant recipients. Three were cadaver transplants and nine were transplants from living related donors. In eight patients there were two first-trimester pregnancy termination, three mid-trimester abortions by hysterotomy, and one spontaneous abortion. Two of the induced abortions were in patients with ileal conduits. There were no unusual major obstetric complications but toxemia of pregnancy and concurrent bacterial and viral infections were common medical problems. Of the liveborn infants, only three of the 12 were small for their gestational age, seven were of appropriate size, and two were large. There were no neonatal problems and no congenital anomalies. Renal function during pregnancy was carefully monitored and was compromised in three of the patients studied. One patient had three pregnancies, including the delivery of a set of twins. Several months following her third delivery she developed fatal hepatitis. Two patients developed carcinoma in situ of the cervix. Pregnancy following renal transplantation is not without risk. Sexually active female transplant recipients should be counseled regarding pregnancy and sterilization should be offered as an option at the time of transplantation.", "contents": "Pregnancy following renal transplantation. From 1968 through 1974 at the University of Minnesota there were 17 pregnancies in 12 patients who were renal transplant recipients. Three were cadaver transplants and nine were transplants from living related donors. In eight patients there were two first-trimester pregnancy termination, three mid-trimester abortions by hysterotomy, and one spontaneous abortion. Two of the induced abortions were in patients with ileal conduits. There were no unusual major obstetric complications but toxemia of pregnancy and concurrent bacterial and viral infections were common medical problems. Of the liveborn infants, only three of the 12 were small for their gestational age, seven were of appropriate size, and two were large. There were no neonatal problems and no congenital anomalies. Renal function during pregnancy was carefully monitored and was compromised in three of the patients studied. One patient had three pregnancies, including the delivery of a set of twins. Several months following her third delivery she developed fatal hepatitis. Two patients developed carcinoma in situ of the cervix. Pregnancy following renal transplantation is not without risk. Sexually active female transplant recipients should be counseled regarding pregnancy and sterilization should be offered as an option at the time of transplantation."}
{"id": "PMID:1101689", "title": "Endothelial survival in corneal tissue stored in M-K medium.", "content": "Cat corneas were stored at refrigerator temperatures in M-K medium (TC-199, 5% dextran), modified M-K medium (TC-199, 1% chondroitin sulfate), or on the intact globe in moist chambers for intervals of one to nine days. Less than 5% of the endothelial cells stained with trypan blue in corneas stored one to four days, regardless of the method of storage. In contrast, after five days, 20% or more of the cells took up the stain in 17 to 21 corneas stored in M-K medium. Although stain uptake indicated nonviability of cells, most of the endothelial cells remained structurally intact when observed by scanning and transmission electron microscopy unless the corneas were incubated in fresh TC-199 for two hours at 35 degrees C before trypan blue staining. In that case, the percentages of disrupted cells observed at each time interval correlated with the percentages of cells that had stained in each cornea. These results indicated that storage in M-K medium did not extend the time donor coreas can be stored before transplantation beyond four days.", "contents": "Endothelial survival in corneal tissue stored in M-K medium. Cat corneas were stored at refrigerator temperatures in M-K medium (TC-199, 5% dextran), modified M-K medium (TC-199, 1% chondroitin sulfate), or on the intact globe in moist chambers for intervals of one to nine days. Less than 5% of the endothelial cells stained with trypan blue in corneas stored one to four days, regardless of the method of storage. In contrast, after five days, 20% or more of the cells took up the stain in 17 to 21 corneas stored in M-K medium. Although stain uptake indicated nonviability of cells, most of the endothelial cells remained structurally intact when observed by scanning and transmission electron microscopy unless the corneas were incubated in fresh TC-199 for two hours at 35 degrees C before trypan blue staining. In that case, the percentages of disrupted cells observed at each time interval correlated with the percentages of cells that had stained in each cornea. These results indicated that storage in M-K medium did not extend the time donor coreas can be stored before transplantation beyond four days."}
{"id": "PMID:1101690", "title": "Comparative pharmacologic effects of pilocarpine administered to normal subjects by eyedrops or by ocular therapeutic systems.", "content": "Normal volunteers used 1, 2, and 4% pilocarpine eyedrops, three times daily, or ocular therapeutic systems placed in the conjunctival cul-de-sac to control intraocular pressure. The systems  continuously release 20 and 40 mug/hour of pilocarpine for one week. Although the amount of drug delivered to the eye from the ocular therapeutic system was one fifth that obtained from the eyedrops, the decrease of intraocular pressure was comparable. The ocular therapeutic systems produced small, constant effects on visual acuity, refractive error, and miosis that did not cause visual handicaps or require correction. The effects of eyedrops on these visual factors were large and varied and produced marked visual handicaps that were not correctable with spectacles. Despite its comparable hypotensive effect in normal subjects, continuous delivery of pilocarpine by means of an ocular therapeutic system elicits less severe side effects than pilocarpine eyedrops.", "contents": "Comparative pharmacologic effects of pilocarpine administered to normal subjects by eyedrops or by ocular therapeutic systems. Normal volunteers used 1, 2, and 4% pilocarpine eyedrops, three times daily, or ocular therapeutic systems placed in the conjunctival cul-de-sac to control intraocular pressure. The systems  continuously release 20 and 40 mug/hour of pilocarpine for one week. Although the amount of drug delivered to the eye from the ocular therapeutic system was one fifth that obtained from the eyedrops, the decrease of intraocular pressure was comparable. The ocular therapeutic systems produced small, constant effects on visual acuity, refractive error, and miosis that did not cause visual handicaps or require correction. The effects of eyedrops on these visual factors were large and varied and produced marked visual handicaps that were not correctable with spectacles. Despite its comparable hypotensive effect in normal subjects, continuous delivery of pilocarpine by means of an ocular therapeutic system elicits less severe side effects than pilocarpine eyedrops."}
{"id": "PMID:1101691", "title": "A erimetric technique believed to test receptive field properties: sequential evaluation glaucoma and other conditions.", "content": "We used a technique to simplify and speed up a perimetric test (Westheimer function). Through sequential testing, the technique was successfully used to evaluate pathologic findings and partially to evaluate treatment regimens in three subjects. One patient had an onset of kinetic field changes and Westheimer function alteration with primary open-angle glaucoma. Temporary remissions of alterations in the Westheimer function and kinetic visual field loss occurred in patients with glaucoma and tobacco-alcohol (complicated) amblyopia. Using these techniques it is possible to localize an anomaly in the outer retinal layer, the inner retinal layer, and central to the optic nerve head. We divided the inner and outer retinal layers on a vascular support basis.", "contents": "A erimetric technique believed to test receptive field properties: sequential evaluation glaucoma and other conditions. We used a technique to simplify and speed up a perimetric test (Westheimer function). Through sequential testing, the technique was successfully used to evaluate pathologic findings and partially to evaluate treatment regimens in three subjects. One patient had an onset of kinetic field changes and Westheimer function alteration with primary open-angle glaucoma. Temporary remissions of alterations in the Westheimer function and kinetic visual field loss occurred in patients with glaucoma and tobacco-alcohol (complicated) amblyopia. Using these techniques it is possible to localize an anomaly in the outer retinal layer, the inner retinal layer, and central to the optic nerve head. We divided the inner and outer retinal layers on a vascular support basis."}
{"id": "PMID:1101693", "title": "The use of previously administered examinations for computer-assisted instruction.", "content": "Five-part multiple choice questions use the same procedure as many programmed texts developed for teaching purposes. However, the latter provide immediate feedback, reinforcing a correct answer and correcting a wrong response. Previously-used quizzes, entered into a computer, can provide a learning experience for new students. The computer responds immediately to each student answer, stating whether it is correct or not. If correct, the reason is presented. If incorrect, the reason is stated, and the correct answer is provided along with an explanation.", "contents": "The use of previously administered examinations for computer-assisted instruction. Five-part multiple choice questions use the same procedure as many programmed texts developed for teaching purposes. However, the latter provide immediate feedback, reinforcing a correct answer and correcting a wrong response. Previously-used quizzes, entered into a computer, can provide a learning experience for new students. The computer responds immediately to each student answer, stating whether it is correct or not. If correct, the reason is presented. If incorrect, the reason is stated, and the correct answer is provided along with an explanation."}
{"id": "PMID:1101695", "title": "Orthodontics and dentistry for the hemophilic patient.", "content": "In this presentation we have contrasted the normal blood-clotting mechanism with the failure to form blood clots in hemophiliacs due to the absence of protein factors necessary for conversion of prothrombin to thrombin. The statistics, hereditary basis, and long-term disabling consequences of hemophilia to the severely ffected patient are described. The systemic means of minimizing severe joint disabilities and serious internal bleeding hazards by employing concentrates of antihemophilic factors to reverse the bleeding defects are discussed. Availability and advantages of the types of concentrates are explained. The fatalistic attitude of hemophiliacs toward hepatitis is discussed, along with admonitions to avoid the use of aspirin, alcohol, and buttock injections. Alternative medications for pain are recommended; and injection sites for pediatric patients are suggested. The details of simplified oral surgical management of hemophilic patients without hospitalization are described, including local anesthetic injection technique, method of performing extractions, general anesthesia techniques when indicated, materials for packing of extraction sockets, regimen and precautions in use of Amicar administration for clot maintenance, postoperative diet, and postsurgical activity guidelines. Also noted is the self-administration of intravenous concentrate infusions at home in the event of hemorrhagin, so that bleeding is on the way to bein controlled even before the patient reaches the hospital. We avoided orthodontic treatment of hemophilic patients in the past; however, recently developed bracket-fixation techniques and auxiliary aids; along with an enlightened understanding that gingival bleeding is ot to be feared, have changed our attitude, and we now treat hemophilic patients in much the same manner as otherwise normal orthodontic patients...", "contents": "Orthodontics and dentistry for the hemophilic patient. In this presentation we have contrasted the normal blood-clotting mechanism with the failure to form blood clots in hemophiliacs due to the absence of protein factors necessary for conversion of prothrombin to thrombin. The statistics, hereditary basis, and long-term disabling consequences of hemophilia to the severely ffected patient are described. The systemic means of minimizing severe joint disabilities and serious internal bleeding hazards by employing concentrates of antihemophilic factors to reverse the bleeding defects are discussed. Availability and advantages of the types of concentrates are explained. The fatalistic attitude of hemophiliacs toward hepatitis is discussed, along with admonitions to avoid the use of aspirin, alcohol, and buttock injections. Alternative medications for pain are recommended; and injection sites for pediatric patients are suggested. The details of simplified oral surgical management of hemophilic patients without hospitalization are described, including local anesthetic injection technique, method of performing extractions, general anesthesia techniques when indicated, materials for packing of extraction sockets, regimen and precautions in use of Amicar administration for clot maintenance, postoperative diet, and postsurgical activity guidelines. Also noted is the self-administration of intravenous concentrate infusions at home in the event of hemorrhagin, so that bleeding is on the way to bein controlled even before the patient reaches the hospital. We avoided orthodontic treatment of hemophilic patients in the past; however, recently developed bracket-fixation techniques and auxiliary aids; along with an enlightened understanding that gingival bleeding is ot to be feared, have changed our attitude, and we now treat hemophilic patients in much the same manner as otherwise normal orthodontic patients..."}
{"id": "PMID:1101703", "title": "Microvascular changes in lymph nodes draining skin allografts.", "content": "Histological, histochemical, ultrastructural, and radiolabeling characteristics of the microvasculature in regional nodes draining skin allograft sites are described. From 12 to 48 hours after grafting, these nodes show increased vascular permeability and altered lymphocyte traffic pattern. The rapid rise in lymphocyte migration indices and the apparent plugging of intermediate sinuses by lymphocytes suggest that both increased entry and decreased egress of recirculating cells contribute in \"lymphocyte trapping.\" This is followed by redistribution of cortical capillary arcades as existing germinal centers dissolve and proliferating lymphocytes infiltrate the cortex. Normal microvascular patterns reappeared at 7 to 14 days as primary and secondary nodules form in the enlarged nodes. Increased length and arborization of high endothelial venules resulted from focal proliferation of endothelial cells in transition zones from high to low endothelium. In stimulated nodes, high endothelial cells exhibit increased cytoplasmic basophilia and acid hydrolase activities which correlate with the appearance of numerous polyribosomes, RER cisternae, and lysosomes in their cytoplasm. These \"activated\" endothelial cells phagocytose microthrombi within venular lumens.", "contents": "Microvascular changes in lymph nodes draining skin allografts. Histological, histochemical, ultrastructural, and radiolabeling characteristics of the microvasculature in regional nodes draining skin allograft sites are described. From 12 to 48 hours after grafting, these nodes show increased vascular permeability and altered lymphocyte traffic pattern. The rapid rise in lymphocyte migration indices and the apparent plugging of intermediate sinuses by lymphocytes suggest that both increased entry and decreased egress of recirculating cells contribute in \"lymphocyte trapping.\" This is followed by redistribution of cortical capillary arcades as existing germinal centers dissolve and proliferating lymphocytes infiltrate the cortex. Normal microvascular patterns reappeared at 7 to 14 days as primary and secondary nodules form in the enlarged nodes. Increased length and arborization of high endothelial venules resulted from focal proliferation of endothelial cells in transition zones from high to low endothelium. In stimulated nodes, high endothelial cells exhibit increased cytoplasmic basophilia and acid hydrolase activities which correlate with the appearance of numerous polyribosomes, RER cisternae, and lysosomes in their cytoplasm. These \"activated\" endothelial cells phagocytose microthrombi within venular lumens."}
{"id": "PMID:1101704", "title": "Glomerular microcapillary thrombosis demonstrated by the new technique of immunocathodoluminescence.", "content": "Fluorescein-labeled antigen-antibody complexes could be made visible by scanning electron microscopy using an intensifying device. This new method of immunocathodoluminescence was demonstrated on cryostat sections of rat kidneys containing glomerular fibrin as the result of endotoxin infusion. The resulting photographs correspond with those obtained by immunofluorescent microscopy. The advantage of this technique is, however, the larger depth of focus. By using thinner cyostat sections it is expected that the higher resolution of scanning microscopy will provide even better details in three dimensions.", "contents": "Glomerular microcapillary thrombosis demonstrated by the new technique of immunocathodoluminescence. Fluorescein-labeled antigen-antibody complexes could be made visible by scanning electron microscopy using an intensifying device. This new method of immunocathodoluminescence was demonstrated on cryostat sections of rat kidneys containing glomerular fibrin as the result of endotoxin infusion. The resulting photographs correspond with those obtained by immunofluorescent microscopy. The advantage of this technique is, however, the larger depth of focus. By using thinner cyostat sections it is expected that the higher resolution of scanning microscopy will provide even better details in three dimensions."}
{"id": "PMID:1101706", "title": "The human mandible: lever or link?", "content": "The mammalian mandible, and in particular the human mandible, is generally thought to function as a lever during biting. This notion, however, has not gone unchallenged. Various workers have suggested that the mandible does not function as a lever, and they base this proposition on essentially two assertions: (1) the resultant of the forces produced by the masticatory muscles always passes through the bite point; (2) the condylar neck and/or the temporomandibular joint is unsuited to withstand reaction forces during biting. A review of the electromyographic data and of the properties of the tissues of the temporomandibular joint do not support the non-lever hypothesis of mandibular function. In addition, an analysis of the strength of the condylar neck demonstrates that this structure is strong enough to withstand the expected reaction force during lever action. Ordinarily the human mandible and the forces that act upon it are analyzed solely in the lateral projection. Moments are then usually analyzed about the mandibular condyle; however, some workers have advocated taking moments about other points, e.g., the instantaneous center of rotation. Obviously it makes no difference as to what point is chosen since the moments about any point during equilibrium conditions are equal to zero. It is also useful to analyze the forces acting on the mandible in the frontal projection, particularly during unilateral biting. The electromyographic data suggest that during powerful unilateral molar biting the resultant adductor muscle force is passing between the bite point and the balancing (non-biting side) condyle. Therefore, in order for this system to be in equilibrium there must be a reaction force acting on the balancing condyle. This suggests that reaction forces are larger on the balancing side than on the working side, and possibly explains why individuals with a painful temporomandibular joint usually prefer to bite on the side of the diseased joint.", "contents": "The human mandible: lever or link? The mammalian mandible, and in particular the human mandible, is generally thought to function as a lever during biting. This notion, however, has not gone unchallenged. Various workers have suggested that the mandible does not function as a lever, and they base this proposition on essentially two assertions: (1) the resultant of the forces produced by the masticatory muscles always passes through the bite point; (2) the condylar neck and/or the temporomandibular joint is unsuited to withstand reaction forces during biting. A review of the electromyographic data and of the properties of the tissues of the temporomandibular joint do not support the non-lever hypothesis of mandibular function. In addition, an analysis of the strength of the condylar neck demonstrates that this structure is strong enough to withstand the expected reaction force during lever action. Ordinarily the human mandible and the forces that act upon it are analyzed solely in the lateral projection. Moments are then usually analyzed about the mandibular condyle; however, some workers have advocated taking moments about other points, e.g., the instantaneous center of rotation. Obviously it makes no difference as to what point is chosen since the moments about any point during equilibrium conditions are equal to zero. It is also useful to analyze the forces acting on the mandible in the frontal projection, particularly during unilateral biting. The electromyographic data suggest that during powerful unilateral molar biting the resultant adductor muscle force is passing between the bite point and the balancing (non-biting side) condyle. Therefore, in order for this system to be in equilibrium there must be a reaction force acting on the balancing condyle. This suggests that reaction forces are larger on the balancing side than on the working side, and possibly explains why individuals with a painful temporomandibular joint usually prefer to bite on the side of the diseased joint."}
{"id": "PMID:1101707", "title": "The immediate effects of chlorpromazine in newly admitted schizophrenic patients.", "content": "To evaluate the clinical effectiveness of chlorpromazine in comparison with placebo over a brief period of hospitalization, the authors conducted a double-blind study of the drug in 44 acutely ill schizophrenic patients. These patients had been newly admitted to a brief treatment unit where the average length of stay was 7 to 10 days. The results of this study indicate that chlorpromazine had no more calming or antipsychotic effect than placebo during the first 5 days of treatment. The authors suggest that 5 days is too short a time for a clinical trial to gauge patient response to chlorpromazine.", "contents": "The immediate effects of chlorpromazine in newly admitted schizophrenic patients. To evaluate the clinical effectiveness of chlorpromazine in comparison with placebo over a brief period of hospitalization, the authors conducted a double-blind study of the drug in 44 acutely ill schizophrenic patients. These patients had been newly admitted to a brief treatment unit where the average length of stay was 7 to 10 days. The results of this study indicate that chlorpromazine had no more calming or antipsychotic effect than placebo during the first 5 days of treatment. The authors suggest that 5 days is too short a time for a clinical trial to gauge patient response to chlorpromazine."}
{"id": "PMID:1101708", "title": "Identification of women at risk for unwanted pregnancy.", "content": "Concern about increasing rates of unwanted pregnancy, particularly among adolescents, has led to consideration of steps necessary to develop effective intervention programs. In order to reach a young woman for counseling and contraceptive assistance before she places herself at risk for unintended pregnancy, it seems necessary to develop the capability for identifying high-risk individuals without reference to sexual history. The authors describe a preliminary attempt to predict contraceptively unprotected coitus from responses to projective test stimuli, noting that the problem is so urgent that even early and necessarily incomplete work in this area may have merit in stimulating further research in identification of women at risk for unwanted pregnancy.", "contents": "Identification of women at risk for unwanted pregnancy. Concern about increasing rates of unwanted pregnancy, particularly among adolescents, has led to consideration of steps necessary to develop effective intervention programs. In order to reach a young woman for counseling and contraceptive assistance before she places herself at risk for unintended pregnancy, it seems necessary to develop the capability for identifying high-risk individuals without reference to sexual history. The authors describe a preliminary attempt to predict contraceptively unprotected coitus from responses to projective test stimuli, noting that the problem is so urgent that even early and necessarily incomplete work in this area may have merit in stimulating further research in identification of women at risk for unwanted pregnancy."}
{"id": "PMID:1101709", "title": "A computer-assisted psychiatric assessment unit.", "content": "The authors discuss the rationale and functional design for an innovative approach to psychiatric intake decision making, stressing the crucial role of an on-line computer support system. The description of the prototype computer-assisted psychiatric assessment process includes an outline of computer and staffing requirements. The authors discuss the initial impact of this psychiatric assessment unit on the hospital's mental health care delivery system.", "contents": "A computer-assisted psychiatric assessment unit. The authors discuss the rationale and functional design for an innovative approach to psychiatric intake decision making, stressing the crucial role of an on-line computer support system. The description of the prototype computer-assisted psychiatric assessment process includes an outline of computer and staffing requirements. The authors discuss the initial impact of this psychiatric assessment unit on the hospital's mental health care delivery system."}
{"id": "PMID:1101710", "title": "Psychiatry in Spain: past and present.", "content": "The authors discuss the contributions and history of psychiatry in Spain on the basis of extensive literature review and on-site observations. The history of Spanish psychiatry is a rich one, including pioneering efforts in the establishment of mental institutions and a strong traditional alliance between psychiatry and literature. However, the authors note that Spanish psychiatrists today are less innovative than their counterparts in other countries. They emphasize the historical and cultural context of the discipline, with illustrations of the importance of the church, attitudes toward the family, and other sociocultural factors.", "contents": "Psychiatry in Spain: past and present. The authors discuss the contributions and history of psychiatry in Spain on the basis of extensive literature review and on-site observations. The history of Spanish psychiatry is a rich one, including pioneering efforts in the establishment of mental institutions and a strong traditional alliance between psychiatry and literature. However, the authors note that Spanish psychiatrists today are less innovative than their counterparts in other countries. They emphasize the historical and cultural context of the discipline, with illustrations of the importance of the church, attitudes toward the family, and other sociocultural factors."}
{"id": "PMID:1101714", "title": "Minimal brain Dysfunction.", "content": "Because of the prevalence in our society of children labeled with the term minimal brain dysfunction; physicians who treat children must develop a point of view toward the syndrome and a working procedure for assisting affected children. An approach to this problem can be divided into two parts: detection of somatic disorders and comprehensive management of school problems. The detection of somatic disorders that may have produced or substantially contributed to the school problem requires conventional medical skills for recognition and treatment. Comprehensive management of school problems requires a broader approach by the physician; including counseling of the parents regarding adverse family interactions, advice about educational resources and consideration of the question of drug therapy for behavioral disorders. In all instances parents should be able to rely on the physician to act as a scientific source of guidance on the merits of the plethora of therapies directed toward the child with learning disorders. The ability to function competently in all of these areas will enable the physician to provide a service for children and their families that may profoundly affect their lives.", "contents": "Minimal brain Dysfunction. Because of the prevalence in our society of children labeled with the term minimal brain dysfunction; physicians who treat children must develop a point of view toward the syndrome and a working procedure for assisting affected children. An approach to this problem can be divided into two parts: detection of somatic disorders and comprehensive management of school problems. The detection of somatic disorders that may have produced or substantially contributed to the school problem requires conventional medical skills for recognition and treatment. Comprehensive management of school problems requires a broader approach by the physician; including counseling of the parents regarding adverse family interactions, advice about educational resources and consideration of the question of drug therapy for behavioral disorders. In all instances parents should be able to rely on the physician to act as a scientific source of guidance on the merits of the plethora of therapies directed toward the child with learning disorders. The ability to function competently in all of these areas will enable the physician to provide a service for children and their families that may profoundly affect their lives."}
{"id": "PMID:1101717", "title": "Increasing acid response to a meal in the chronic Heidenhain pouch.", "content": "Heidenhain pouches were established in four mongrel dogs. Acid secretory values were studied in response to a meal, both after operation and six to eight months later. Large increases in the acid responses of the Heidenhain pouch to a meal were noted over a period of time. Mechanisms accountable for this acid increase were studied, including serum gastrin response, pepsin activity, and morphologic changes in the pouch. Regeneration or reinnervation of vagal fibers may occur at a subthreshold level but is not well documented. The necessity of repeated control studies in long-term evaluation using the Heidenhain pouch is emphasized.", "contents": "Increasing acid response to a meal in the chronic Heidenhain pouch. Heidenhain pouches were established in four mongrel dogs. Acid secretory values were studied in response to a meal, both after operation and six to eight months later. Large increases in the acid responses of the Heidenhain pouch to a meal were noted over a period of time. Mechanisms accountable for this acid increase were studied, including serum gastrin response, pepsin activity, and morphologic changes in the pouch. Regeneration or reinnervation of vagal fibers may occur at a subthreshold level but is not well documented. The necessity of repeated control studies in long-term evaluation using the Heidenhain pouch is emphasized."}
{"id": "PMID:1101718", "title": "Kidney transplantation in patients with end stage congenital renal disease. Report of eighteen cases and review of the Organ Transplant Registry.", "content": "Eighteen patients with end stage congenital renal disease requiring kidney transplantation constituted 12 per cent of the transplantation recipients at this institution over the past six years. The post-transplantation course in this group was remarkably satisfactory, with a 94 per cent graft survival at three years. In addition, we analyzed survival data from over 9,900 patients in the Organ Transplant Registry and demonstrated that transplant recipients with end stage congenital renal disease have equal or better five year patient survival compared with those with acquired end stage renal disease. Only those patients with adult polycystic disease had a less satisfactory prognosis, probably because of age-related factors. In contrast, there were few statistical correlations between renal allograft survival, age, and original disease.", "contents": "Kidney transplantation in patients with end stage congenital renal disease. Report of eighteen cases and review of the Organ Transplant Registry. Eighteen patients with end stage congenital renal disease requiring kidney transplantation constituted 12 per cent of the transplantation recipients at this institution over the past six years. The post-transplantation course in this group was remarkably satisfactory, with a 94 per cent graft survival at three years. In addition, we analyzed survival data from over 9,900 patients in the Organ Transplant Registry and demonstrated that transplant recipients with end stage congenital renal disease have equal or better five year patient survival compared with those with acquired end stage renal disease. Only those patients with adult polycystic disease had a less satisfactory prognosis, probably because of age-related factors. In contrast, there were few statistical correlations between renal allograft survival, age, and original disease."}
{"id": "PMID:1101719", "title": "Transcutaneous electrical stimulation in treatment of postoperative pain.", "content": "Transcutaneous electrical stimulation as a means of relieving acute pain was evaluated in 100 patients who underwent abdominal or thoracic operative procedures. Transcutaneous electrical stimulation proveded pain relief in 77 per cent of test patients as opposed to 17 per cent of control patients. Transcutaneous electrical stimulation is an effective method of treatment that has many advantages over analgesic drugs.", "contents": "Transcutaneous electrical stimulation in treatment of postoperative pain. Transcutaneous electrical stimulation as a means of relieving acute pain was evaluated in 100 patients who underwent abdominal or thoracic operative procedures. Transcutaneous electrical stimulation proveded pain relief in 77 per cent of test patients as opposed to 17 per cent of control patients. Transcutaneous electrical stimulation is an effective method of treatment that has many advantages over analgesic drugs."}
{"id": "PMID:1101720", "title": "Successful management of miliary tuberculosis after renal transplantation.", "content": "Miliary tuberculosis is the most lethal form of tubercular disease. If dissemination of tubercle bacilli occurs without therapy, death is almost certain. The importance of establishing an etiologic diagnosis as promptly as possible in patients receiving immunosuppressive therapy is self-explanatory. The presence of a life-threatening infection in these patients requires aggressive antimicrobial therapy and discontinuation of the immunosuppressive drugs until the infectious process is under control; the presence of an impaired immunologic response is responsible for the life-threatening infection and the lack of an acute rejection reaction.", "contents": "Successful management of miliary tuberculosis after renal transplantation. Miliary tuberculosis is the most lethal form of tubercular disease. If dissemination of tubercle bacilli occurs without therapy, death is almost certain. The importance of establishing an etiologic diagnosis as promptly as possible in patients receiving immunosuppressive therapy is self-explanatory. The presence of a life-threatening infection in these patients requires aggressive antimicrobial therapy and discontinuation of the immunosuppressive drugs until the infectious process is under control; the presence of an impaired immunologic response is responsible for the life-threatening infection and the lack of an acute rejection reaction."}
{"id": "PMID:1101721", "title": "The \"parachute\" stent.", "content": "A method of stabilizing intraoral skin grafts from outside the mouth is described. It provides excellent immobilization ot the graft and superior oral hygiene as compared with conventional intraoral stents.", "contents": "The \"parachute\" stent. A method of stabilizing intraoral skin grafts from outside the mouth is described. It provides excellent immobilization ot the graft and superior oral hygiene as compared with conventional intraoral stents."}
{"id": "PMID:1101723", "title": "Late sequelae of radiation therapy in cancer of the head and neck with particular reference to the nasopharynx.", "content": "Sequlae of radiation therapy may be late in occurring and varied in their manifestations. Although some are untreatable and progressive, the risk of development of some other sequelae can be minimized by careful application of radiotherapy or by ancillary measures, such as dental decay prophylaxis. Some of the serious sequelae secondary to radiation therapy of the nasopharynx have been summarized. These include radiation myelitis, paralysis of the cranial nerves, stricture of the pharynx, radiation-induced cancer, and necrosis with fatal hemorrhage.", "contents": "Late sequelae of radiation therapy in cancer of the head and neck with particular reference to the nasopharynx. Sequlae of radiation therapy may be late in occurring and varied in their manifestations. Although some are untreatable and progressive, the risk of development of some other sequelae can be minimized by careful application of radiotherapy or by ancillary measures, such as dental decay prophylaxis. Some of the serious sequelae secondary to radiation therapy of the nasopharynx have been summarized. These include radiation myelitis, paralysis of the cranial nerves, stricture of the pharynx, radiation-induced cancer, and necrosis with fatal hemorrhage."}
{"id": "PMID:1101724", "title": "T-cell deficiency in patients with squamous cell cancer of the head and neck.", "content": "Cellular immunity was assessed in patients with operable squamous cell cancer of the head and neck using in vivo skin tests and in vitro lymphocyte stimulation tests. An expansion of a previous study continued to show that 30 per cent of patients with T1N0M0 lesions were DNCB-negative and that with more advanced lesions there was further impairment. A similar finding was observed in the blastogenic response to phytohemagglutinin and concanavalin A but not pokeweed mitogen. Overall, 40 per cent of patients with resectable cancer had a significant depression of the blastogenic responses to conconavalin A and phytohemagglutinin. This depression ranged from 15 per cent in patients with T1N0M0 lesions to 71 per cent in those with T3N0M0 lesions. Although this depression was more severe in patients with palpable cervical node metastases, it was related more to the size of the primary tumor than to the nodes per se. An exception occurred in patients with large fixed nodes in whom the depression of lymphocyte stimulation was most severe. The absolute T-cell count was also depressed in patients with head and neck cancer. This depression parallelled the lymphocyte stimulation results with phytohemagglutinin and conconavalin A and was progressive with increasing stage of disease. A correlation exists between DNCB negativity and early recurrence and shortened survival. Clinical follow-up study is too short to assess the correlation of in vitro immune function with these clinical prognostic factors.", "contents": "T-cell deficiency in patients with squamous cell cancer of the head and neck. Cellular immunity was assessed in patients with operable squamous cell cancer of the head and neck using in vivo skin tests and in vitro lymphocyte stimulation tests. An expansion of a previous study continued to show that 30 per cent of patients with T1N0M0 lesions were DNCB-negative and that with more advanced lesions there was further impairment. A similar finding was observed in the blastogenic response to phytohemagglutinin and concanavalin A but not pokeweed mitogen. Overall, 40 per cent of patients with resectable cancer had a significant depression of the blastogenic responses to conconavalin A and phytohemagglutinin. This depression ranged from 15 per cent in patients with T1N0M0 lesions to 71 per cent in those with T3N0M0 lesions. Although this depression was more severe in patients with palpable cervical node metastases, it was related more to the size of the primary tumor than to the nodes per se. An exception occurred in patients with large fixed nodes in whom the depression of lymphocyte stimulation was most severe. The absolute T-cell count was also depressed in patients with head and neck cancer. This depression parallelled the lymphocyte stimulation results with phytohemagglutinin and conconavalin A and was progressive with increasing stage of disease. A correlation exists between DNCB negativity and early recurrence and shortened survival. Clinical follow-up study is too short to assess the correlation of in vitro immune function with these clinical prognostic factors."}
{"id": "PMID:1101725", "title": "Carcinoma of the lip.", "content": "A retrospective analysis of 206 patients with lip cancer is presented. Poorly differentiated primary lesions have a high rate of metastases. The metastatic potential of well differentiated lesions, when classified by size in the TNM staging system, is the same as that of intraoral carcinoma. A therapeutic approach is proposed.", "contents": "Carcinoma of the lip. A retrospective analysis of 206 patients with lip cancer is presented. Poorly differentiated primary lesions have a high rate of metastases. The metastatic potential of well differentiated lesions, when classified by size in the TNM staging system, is the same as that of intraoral carcinoma. A therapeutic approach is proposed."}
{"id": "PMID:1101726", "title": "Nasolabial flap reconstruction of the floor of the mouth after extirpation of oral cancer.", "content": "The transbuccal nasolabial flap is an extremely valuable method for primary or secondary reconstruction of the floor of the mouth. We urge its frequent use.", "contents": "Nasolabial flap reconstruction of the floor of the mouth after extirpation of oral cancer. The transbuccal nasolabial flap is an extremely valuable method for primary or secondary reconstruction of the floor of the mouth. We urge its frequent use."}
{"id": "PMID:1101727", "title": "[Corneal lesions in ichthyosis (author's transl)].", "content": "The authors report about observations they made in two sisters. One sister showed a fibroid degeneration of the cornea, the other a band-shaped keratopathy respectively, together with an ichthyosis and an alopecia as a result of capillary fractures due to pili torti. An autosomal recessive hereditary transmission could be determined. The cutaneous lesion is either an ichthyosis vulgaris, the hereditary transmission of which could not yet be confirmed, or it is a transition form of ichthyosis vulgaris and congenita.", "contents": "[Corneal lesions in ichthyosis (author's transl)]. The authors report about observations they made in two sisters. One sister showed a fibroid degeneration of the cornea, the other a band-shaped keratopathy respectively, together with an ichthyosis and an alopecia as a result of capillary fractures due to pili torti. An autosomal recessive hereditary transmission could be determined. The cutaneous lesion is either an ichthyosis vulgaris, the hereditary transmission of which could not yet be confirmed, or it is a transition form of ichthyosis vulgaris and congenita."}
{"id": "PMID:1101728", "title": "New Zealand's first general anaesthetic.", "content": "The administration of New Zealand's first general anaesthetic took place at the Colonial Gaol, Wellington, on the morning of Monday, September 27th, 1847. The agent used was sulphuric ether which was administered by Mr. Marriot, the manufacturer of the Herapath-type inhaler used on this occasion. The operation, a dental extraction, was performed by the Colonial Surgeon, Dr. J. P. Fitzgerald.", "contents": "New Zealand's first general anaesthetic. The administration of New Zealand's first general anaesthetic took place at the Colonial Gaol, Wellington, on the morning of Monday, September 27th, 1847. The agent used was sulphuric ether which was administered by Mr. Marriot, the manufacturer of the Herapath-type inhaler used on this occasion. The operation, a dental extraction, was performed by the Colonial Surgeon, Dr. J. P. Fitzgerald."}
{"id": "PMID:1101729", "title": "Anaesthetic considerations in chronic renal failure.", "content": "This paper discusses the anaesthetic problems presented by patients in chronic renal failure. The anaesthetic agents and techniques used in 181 patients who underwent 941 operations (including 218 renal transplants) are described.", "contents": "Anaesthetic considerations in chronic renal failure. This paper discusses the anaesthetic problems presented by patients in chronic renal failure. The anaesthetic agents and techniques used in 181 patients who underwent 941 operations (including 218 renal transplants) are described."}
{"id": "PMID:1101730", "title": "Chest physiotherapy during mechanical ventilation.", "content": "The effects of three different physiotherapy techniques on arterial oxygen tensions and A-aO2 gradients were evaluated in 17 patients subjected to mechanical ventilation. No significant changes were detected and the clinical significance of these findings, which contrast with those of other investigators, is discussed.", "contents": "Chest physiotherapy during mechanical ventilation. The effects of three different physiotherapy techniques on arterial oxygen tensions and A-aO2 gradients were evaluated in 17 patients subjected to mechanical ventilation. No significant changes were detected and the clinical significance of these findings, which contrast with those of other investigators, is discussed."}
{"id": "PMID:1101731", "title": "A simple clinical method of quantitating the effects of chest physiotherapy in mechanically ventilated patients.", "content": "A simple clinical method, allowing quantitive assessment of the effects of chest physiotherapy on patients subjected to I.P.P.V., is presented. The method involves measuring of the \"alveolar\" pressure using the end-expiratory retard mechanism available on the Bennet MA 1 (volume cycled) ventilator. Using this simple technique three different methods of physiotherapy were shown to significantly lower alveolar pressure, indicating an increase in pulmonary compliance. The inhalation of acetyl cysteine however, appeared to render treatment less effective in this regard.", "contents": "A simple clinical method of quantitating the effects of chest physiotherapy in mechanically ventilated patients. A simple clinical method, allowing quantitive assessment of the effects of chest physiotherapy on patients subjected to I.P.P.V., is presented. The method involves measuring of the \"alveolar\" pressure using the end-expiratory retard mechanism available on the Bennet MA 1 (volume cycled) ventilator. Using this simple technique three different methods of physiotherapy were shown to significantly lower alveolar pressure, indicating an increase in pulmonary compliance. The inhalation of acetyl cysteine however, appeared to render treatment less effective in this regard."}
{"id": "PMID:1101733", "title": "The origin and fate of osteoclasts.", "content": "Despite intensive and ingenious investigation, the origins and ultimate fate of the osteoclast remain shrouded in mystery. This brief review evaluates some of the recent experimental approaches used in the study of the osteoclast, especially whether they form from intra- or extra-skeletal progenitor cells, whether from the same osteoprogenitor cell as the osteoblast, and whether, once formed, they may modulate to osteoblasts. That osteoprogenitor cells can, and do, become osteoclasts is well founded, as is the conclusion that such progenitor cells originate as blood-borne, extra-skeletal cells. Evidence that sessile, intra-skeletal, progenitor cells can form osteoclasts is less direct. There is good evidence that osteoclasts both shed and take-up nuclei, but no direct evidence that nuclear shedding is accompanied by death of the osteoclast, and no direct evidence for the fate of the shed nuclei. Whether the same osteoprogenitor cell can produce either an osteoblast or an osteoclast also remains an open question.", "contents": "The origin and fate of osteoclasts. Despite intensive and ingenious investigation, the origins and ultimate fate of the osteoclast remain shrouded in mystery. This brief review evaluates some of the recent experimental approaches used in the study of the osteoclast, especially whether they form from intra- or extra-skeletal progenitor cells, whether from the same osteoprogenitor cell as the osteoblast, and whether, once formed, they may modulate to osteoblasts. That osteoprogenitor cells can, and do, become osteoclasts is well founded, as is the conclusion that such progenitor cells originate as blood-borne, extra-skeletal cells. Evidence that sessile, intra-skeletal, progenitor cells can form osteoclasts is less direct. There is good evidence that osteoclasts both shed and take-up nuclei, but no direct evidence that nuclear shedding is accompanied by death of the osteoclast, and no direct evidence for the fate of the shed nuclei. Whether the same osteoprogenitor cell can produce either an osteoblast or an osteoclast also remains an open question."}
{"id": "PMID:1101753", "title": "Urinary metabolites of halothane in man.", "content": "The urinary metabolites of halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) were investigated in five individuals given trace doses (25 muCi), and in three individuals given large doses (1 mCi) of radioactively labeled 14C-halothane. The latter were donor subjects for heart transplant operations. Separation of the nonvolatile urinary metabolites of halothane was accomplished by chemical extraction, electrophoresis, ion-exchange and high-pressure liquid chromatography, and gas chromatography. Identification of the individual metabolites was by nuclear magnetic resonance and mass spectrometry. Three major metabolites were identified: trifluoroacetic acid, N-trifluoroacetyl-2-aminoethanol, and N-acetyl-S-(2-bromo-2-chloro-1,1-difluoroethyl)-L-cysteine. Smaller unidentified radioactive peaks were also found. The presence of both ethanolamide and cysteine conjugates of halothane is of concern. These urinary products imply the presence of reactive intermediates. The conjugation of such intermediates to proteins and phospholipids may give rise to the high-molecular-weight covalently bound metabolites demonstrated to be present in the liver following halothane anesthesia. Elucidation of the structures of the urinary metabolites provides information important to an understanding of halothane metabolism and its potential hepatotoxicity.", "contents": "Urinary metabolites of halothane in man. The urinary metabolites of halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) were investigated in five individuals given trace doses (25 muCi), and in three individuals given large doses (1 mCi) of radioactively labeled 14C-halothane. The latter were donor subjects for heart transplant operations. Separation of the nonvolatile urinary metabolites of halothane was accomplished by chemical extraction, electrophoresis, ion-exchange and high-pressure liquid chromatography, and gas chromatography. Identification of the individual metabolites was by nuclear magnetic resonance and mass spectrometry. Three major metabolites were identified: trifluoroacetic acid, N-trifluoroacetyl-2-aminoethanol, and N-acetyl-S-(2-bromo-2-chloro-1,1-difluoroethyl)-L-cysteine. Smaller unidentified radioactive peaks were also found. The presence of both ethanolamide and cysteine conjugates of halothane is of concern. These urinary products imply the presence of reactive intermediates. The conjugation of such intermediates to proteins and phospholipids may give rise to the high-molecular-weight covalently bound metabolites demonstrated to be present in the liver following halothane anesthesia. Elucidation of the structures of the urinary metabolites provides information important to an understanding of halothane metabolism and its potential hepatotoxicity."}
{"id": "PMID:1101755", "title": "Comparison of heparin and streptokinase in the treatment of venous thrombosis.", "content": "Heparin or streptokinase was administered in a prospective randomized fashion to 50 patients with phlebographically confirmed venous thrombosis of the extremities of 14 days or less duration. A total of 49 patients completed the investigative protocol with 26 receiving heparin and 23 receiving streptokinase. All patients were evaluated with sequential phlebograms.  Complete thrombolysis with restoration of venous valve function occurred in one of 26 patients receiving heparin and in six of 23 patients receiving streptokinase. Fifty per cent of the patients treated with streptokinase with a total duration of symptoms of three days or less achieved complete lysis. The total incidence of therapeutic complications was similar in the two groups, but was more severe in the streptokinase treated patients.", "contents": "Comparison of heparin and streptokinase in the treatment of venous thrombosis. Heparin or streptokinase was administered in a prospective randomized fashion to 50 patients with phlebographically confirmed venous thrombosis of the extremities of 14 days or less duration. A total of 49 patients completed the investigative protocol with 26 receiving heparin and 23 receiving streptokinase. All patients were evaluated with sequential phlebograms.  Complete thrombolysis with restoration of venous valve function occurred in one of 26 patients receiving heparin and in six of 23 patients receiving streptokinase. Fifty per cent of the patients treated with streptokinase with a total duration of symptoms of three days or less achieved complete lysis. The total incidence of therapeutic complications was similar in the two groups, but was more severe in the streptokinase treated patients."}
{"id": "PMID:1101756", "title": "Management of amputation-stump breakdown.", "content": "Seven cases of above-knee amputation stump breakdown following surgery for arteriosclerosis obliterans were successfully treated with Travase ointment. (Bacillus subtilis proteinase). Rehabilitation was facilitated because secondary closure of the previously infected necrotic wounds could be more rapidly undertaken.", "contents": "Management of amputation-stump breakdown. Seven cases of above-knee amputation stump breakdown following surgery for arteriosclerosis obliterans were successfully treated with Travase ointment. (Bacillus subtilis proteinase). Rehabilitation was facilitated because secondary closure of the previously infected necrotic wounds could be more rapidly undertaken."}
{"id": "PMID:1101757", "title": "Combined propranolol and digoxin therapy in angina pectoris.", "content": "The effects of oral propranolol and digoxin and digoxin alone and in combination on angina frequency, heart size, systolic time intervals and treadmill exercise tolerance, were assessed in 20 patients with coronary heart disease. Oral propranolol alone reduced the average frequency of angina pectoris from 16 to 7 attacks per week (P less than 0.02). However, the mean duration of exercise was not significantly improved because 8 patients with abnormal left ventricular function exhibited a decrease in exercise tolerance. Combined propranolol and digoxin improved exercise tolerance in these patients, and, consequently, mean exercise duration in all patients increased significantly from the control value of 390 +/- 42 to 458 +/- 46 s (P less than 0.01). Propranolol alone also resulted in a significant increase in left heart size from 46.5 +/- 1.3 to 47.7 +/- 1.5 mm/m2 (P less than 0.001), which was reversed by the addition of digoxin. Therefore, oral propranolol combined with digoxin is advantageous in patients with angina pectoris who have abnormal ventricular function or large hearts.", "contents": "Combined propranolol and digoxin therapy in angina pectoris. The effects of oral propranolol and digoxin and digoxin alone and in combination on angina frequency, heart size, systolic time intervals and treadmill exercise tolerance, were assessed in 20 patients with coronary heart disease. Oral propranolol alone reduced the average frequency of angina pectoris from 16 to 7 attacks per week (P less than 0.02). However, the mean duration of exercise was not significantly improved because 8 patients with abnormal left ventricular function exhibited a decrease in exercise tolerance. Combined propranolol and digoxin improved exercise tolerance in these patients, and, consequently, mean exercise duration in all patients increased significantly from the control value of 390 +/- 42 to 458 +/- 46 s (P less than 0.01). Propranolol alone also resulted in a significant increase in left heart size from 46.5 +/- 1.3 to 47.7 +/- 1.5 mm/m2 (P less than 0.001), which was reversed by the addition of digoxin. Therefore, oral propranolol combined with digoxin is advantageous in patients with angina pectoris who have abnormal ventricular function or large hearts."}
{"id": "PMID:1101758", "title": "Naproxen in rheumatoid arthritis. A controlled trial.", "content": "The efficacy of naproxen in treating rheumatoid arthritis patients was evaluated in a double-blind clinical trial using aspirin as the control drug. The study was conducted at seven centers and involved 80 patients. After an unequivocal increase in disease activity during a drug-free period, patients were randomly assigned to either drug and continued in the trial for 16 weeks. Some patients took low maintenance doses of corticosteroids, or gold salts, or both throughout the trial. Both test drugs significantly decreased disease activity as measured by a number of ways. By objective measurements, naproxen was as effective as aspirin, although patients in the naproxen-treated group entered the trial with more severe disease. By some subjective evaluations, naproxen was considered more effective than aspirin. Although patients taking naproxen had less frequent gastrointestinal side effects and fewer symptoms VIIIth nerve toxicity, the differences were not statistically significant. We conclude that naproxen is a useful addition to the physician's armamentarium for the treatment of rheumatoid arthritis.", "contents": "Naproxen in rheumatoid arthritis. A controlled trial. The efficacy of naproxen in treating rheumatoid arthritis patients was evaluated in a double-blind clinical trial using aspirin as the control drug. The study was conducted at seven centers and involved 80 patients. After an unequivocal increase in disease activity during a drug-free period, patients were randomly assigned to either drug and continued in the trial for 16 weeks. Some patients took low maintenance doses of corticosteroids, or gold salts, or both throughout the trial. Both test drugs significantly decreased disease activity as measured by a number of ways. By objective measurements, naproxen was as effective as aspirin, although patients in the naproxen-treated group entered the trial with more severe disease. By some subjective evaluations, naproxen was considered more effective than aspirin. Although patients taking naproxen had less frequent gastrointestinal side effects and fewer symptoms VIIIth nerve toxicity, the differences were not statistically significant. We conclude that naproxen is a useful addition to the physician's armamentarium for the treatment of rheumatoid arthritis."}
{"id": "PMID:1101759", "title": "Progesterone for outpatient treatment of Pickwickian syndrome.", "content": "Ten patients with the Pickwickian syndrome, characterized by obesity, hypoxemia, hypercapnia, polycythemia, and cor pulmonale, underwent long-term treatment as outpatients with medroxyprogesterone acetate. Although there was no significant weight change in the group, PaO2 rose 12.6 +/- 2.7 mm Hg (SEM) from 49 +/- 2.6 mm Hg to 62 +/- 2.3 mm Hg (P less than 0.001), while PaCO2 fell 13 +/- 2.6 mm Hg from 51 +/- 1.9 mm Hg to 38 +/- 1.2 mm Hg (P less than 0.001). Hematocrit fell from 56 +/- 2.5% to 50 +/- 1.2%, a mean fall of 6% (P less than 0.01), during medroxyprogesterone acetate therapy. In the 2 patients who had cardiac catheterization before and during medroxyprogesterone acetate therapy, mean pulmonary arterial pressure fell 13 and 19 mm Hg. There were no recurrences of cor pulmonale during treatment. These effects on arterial blood gas values and clinical state were sustained during therapy. On withdrawal of medroxyprogesterone acetate during 1-month period, arterial oxygen and carbon dioxide tensions deteriorated to their previous pretreatment values. Reinstitution of medroxyprogesterone acetate caused improvement in both the oxygen and carbon dioxide tensions. We conclude that sublingual medroxyprogesterone acetate therapy is useful in the management of the Pickwickian syndrome.", "contents": "Progesterone for outpatient treatment of Pickwickian syndrome. Ten patients with the Pickwickian syndrome, characterized by obesity, hypoxemia, hypercapnia, polycythemia, and cor pulmonale, underwent long-term treatment as outpatients with medroxyprogesterone acetate. Although there was no significant weight change in the group, PaO2 rose 12.6 +/- 2.7 mm Hg (SEM) from 49 +/- 2.6 mm Hg to 62 +/- 2.3 mm Hg (P less than 0.001), while PaCO2 fell 13 +/- 2.6 mm Hg from 51 +/- 1.9 mm Hg to 38 +/- 1.2 mm Hg (P less than 0.001). Hematocrit fell from 56 +/- 2.5% to 50 +/- 1.2%, a mean fall of 6% (P less than 0.01), during medroxyprogesterone acetate therapy. In the 2 patients who had cardiac catheterization before and during medroxyprogesterone acetate therapy, mean pulmonary arterial pressure fell 13 and 19 mm Hg. There were no recurrences of cor pulmonale during treatment. These effects on arterial blood gas values and clinical state were sustained during therapy. On withdrawal of medroxyprogesterone acetate during 1-month period, arterial oxygen and carbon dioxide tensions deteriorated to their previous pretreatment values. Reinstitution of medroxyprogesterone acetate caused improvement in both the oxygen and carbon dioxide tensions. We conclude that sublingual medroxyprogesterone acetate therapy is useful in the management of the Pickwickian syndrome."}
{"id": "PMID:1101760", "title": "Bullous dermatosis of hemodialysis.", "content": "Five patients with chronic renal failure who were undergoing maintenance hemodialysis developed a self-limited bullous dermatosis restricted to light exposed areas, primarily involving the dorsa of the hands. Porphyrin levels and phototesting were normal, but histologic sections of the bullae showed thickening of venule walls, hypogranulated mast cells, and perivenular deposition of immunoglobulins. This combination of features has not previously been described and may reflect a pathologic process unique to chronic hemodialysis.", "contents": "Bullous dermatosis of hemodialysis. Five patients with chronic renal failure who were undergoing maintenance hemodialysis developed a self-limited bullous dermatosis restricted to light exposed areas, primarily involving the dorsa of the hands. Porphyrin levels and phototesting were normal, but histologic sections of the bullae showed thickening of venule walls, hypogranulated mast cells, and perivenular deposition of immunoglobulins. This combination of features has not previously been described and may reflect a pathologic process unique to chronic hemodialysis."}
{"id": "PMID:1101761", "title": "Amikacin therapy for severe gram-negative sepsis. Emphasis on infections with gentamicin-resistant organisms.", "content": "Amikacin (BB-K8) is a semisynthetic derivative of kanamycin which is active in vitro against many gentamicin-resistant Gram-negative bacilli. Twenty-three patients with 25 serious Gram-negative infections were treated with this new aminoglycoside. Twelve infections involved organisms that were resistant to gentamicin. Twenty patients satisfied the criteria for bacteriological and clinical cure. This included 11 of the 12 infections involving gentamicin-resistant Gram-negative bacilli. In 4 urinary tract infections there was a good clinical response, but routine follow-up urine cultures at 30 days were positive. One patient failed on amikacin therapy. Eighth nerve toxicity was detected in two patients. These results indicate that amikacin is effective in the treatment of serious Gram-negative infections and is particularly useful in those involving resistant organisms. Further studies are indicated to evaluate ototoxic potential.", "contents": "Amikacin therapy for severe gram-negative sepsis. Emphasis on infections with gentamicin-resistant organisms. Amikacin (BB-K8) is a semisynthetic derivative of kanamycin which is active in vitro against many gentamicin-resistant Gram-negative bacilli. Twenty-three patients with 25 serious Gram-negative infections were treated with this new aminoglycoside. Twelve infections involved organisms that were resistant to gentamicin. Twenty patients satisfied the criteria for bacteriological and clinical cure. This included 11 of the 12 infections involving gentamicin-resistant Gram-negative bacilli. In 4 urinary tract infections there was a good clinical response, but routine follow-up urine cultures at 30 days were positive. One patient failed on amikacin therapy. Eighth nerve toxicity was detected in two patients. These results indicate that amikacin is effective in the treatment of serious Gram-negative infections and is particularly useful in those involving resistant organisms. Further studies are indicated to evaluate ototoxic potential."}
{"id": "PMID:1101762", "title": "Effect of carbon monoxide on maximal treadmill exercise. A study in normal persons.", "content": "The effect of breathing 100 ppm of carbon monoxide versus compressed, purified air for 1 h on maximal treadmill exericse was studied (double-blind crossover design) in 10 middle-aged, healthy nonsmokers. The mean venous carboxyhemoglobin level significantly increased from 1.67% to 3.95%  after breathing carbon monoxide (P less than 0.001) and significantly decreased from 1.63% to 1.30% after breathing compressed, purified air (P less than 0.001). The mean exercise time until exhaustion significantly decreased from 697.7 to 662.7 s after breathing carbon monoxide (P less than 0.001) and insignificantly increased from 694.9 s to 703.4 s after breathing compressed, purified air. Ischemic S-T segment depression larger than or equal to 1.0 mm after exercise occurred in 1 of 10 subjects after exercise following carbon monoxide inhalation. Increased carboxyhemoglobin levels of the magnitude encountered after smoking or heavy atmospheric pollution impair exercise performance in normal persons.", "contents": "Effect of carbon monoxide on maximal treadmill exercise. A study in normal persons. The effect of breathing 100 ppm of carbon monoxide versus compressed, purified air for 1 h on maximal treadmill exericse was studied (double-blind crossover design) in 10 middle-aged, healthy nonsmokers. The mean venous carboxyhemoglobin level significantly increased from 1.67% to 3.95%  after breathing carbon monoxide (P less than 0.001) and significantly decreased from 1.63% to 1.30% after breathing compressed, purified air (P less than 0.001). The mean exercise time until exhaustion significantly decreased from 697.7 to 662.7 s after breathing carbon monoxide (P less than 0.001) and insignificantly increased from 694.9 s to 703.4 s after breathing compressed, purified air. Ischemic S-T segment depression larger than or equal to 1.0 mm after exercise occurred in 1 of 10 subjects after exercise following carbon monoxide inhalation. Increased carboxyhemoglobin levels of the magnitude encountered after smoking or heavy atmospheric pollution impair exercise performance in normal persons."}
{"id": "PMID:1101764", "title": "Mammalian somatic hybrids and human gene mapping.", "content": "Somatic cell hybridization, as an aid in the study of human map, has made large strides during the past several years. Rodent-human hybrids are readily obtained, and selective systems are available to select hybrid cells to the exclusion of parenteral cells. Various species-specific genetic markers can be studied in the hybrids, and correlation of the expression of these markers with each other and with specific human chromosomes retained allows synteny analysis and chromosome assignment. Methods to determine the relative order of genes and their regional localization are also available. Using these methods, more than 60 human genes have been assigned to 22 human chromosomes.", "contents": "Mammalian somatic hybrids and human gene mapping. Somatic cell hybridization, as an aid in the study of human map, has made large strides during the past several years. Rodent-human hybrids are readily obtained, and selective systems are available to select hybrid cells to the exclusion of parenteral cells. Various species-specific genetic markers can be studied in the hybrids, and correlation of the expression of these markers with each other and with specific human chromosomes retained allows synteny analysis and chromosome assignment. Methods to determine the relative order of genes and their regional localization are also available. Using these methods, more than 60 human genes have been assigned to 22 human chromosomes."}
{"id": "PMID:1101765", "title": "How Korotkoff, the surgeon, discovered the auscultatory method of measuring arterial pressure.", "content": "The Korotkoff sounds that measure systolic and diastolic arterial pressure levels were described in a brief communication by N.C. Korotkoff in 1905. He did not state how it happened that he discovered this auscultatory method of measuring \"blood\" pressure. The search for information on this matter led to studying the thesis for his doctorate published in 1910 on Experiments for Determining the Efficiency of Arterial Collaterals. It seems that when he wanted to be sure that pressureonan artery (digital, or with an Esmarch ligature or a Riva-Rocci cuff) had completely obliterated its lumen, he listened over the artery below the area of pressure. The absence of any sounds meant complete obliteration; a sound appeared with each beat when some flow did occur during partial obliteration of the lumen and when no pulsation could be detected because of the small volume of flow.", "contents": "How Korotkoff, the surgeon, discovered the auscultatory method of measuring arterial pressure. The Korotkoff sounds that measure systolic and diastolic arterial pressure levels were described in a brief communication by N.C. Korotkoff in 1905. He did not state how it happened that he discovered this auscultatory method of measuring \"blood\" pressure. The search for information on this matter led to studying the thesis for his doctorate published in 1910 on Experiments for Determining the Efficiency of Arterial Collaterals. It seems that when he wanted to be sure that pressureonan artery (digital, or with an Esmarch ligature or a Riva-Rocci cuff) had completely obliterated its lumen, he listened over the artery below the area of pressure. The absence of any sounds meant complete obliteration; a sound appeared with each beat when some flow did occur during partial obliteration of the lumen and when no pulsation could be detected because of the small volume of flow."}
{"id": "PMID:1101767", "title": "Microfluorometric immunoassays with antigens bound to sepharose beads.", "content": "Sepharose beads, to which protein had been coupled, were used in the development of two types of microfluorometric immunoassays. The first is an inhibition assay: a fixed amount of antiserum was absorbed with different amounts of antigen. This was accomplished in both direct and indirect incubation schemes. The system has been studied with human IgG and mouse IgM as an example. The second is a competition assay: fluorescein-labeled ovalbumin competea with unlabeled unlabeled ovalbumin for the binding sites of a rabbit antiovalbumin. This antiovalbumin was not bound directly to Sepharose but to Sepharose-coupled ovalbumin. In the indirect fluorescence inhibition and the competition assay, the sensitivity levels were determined. They proved to be the same, namely, 25 ng. This means that these fluorescence immunoassays are 10 times less sensitive than conventional radioimmunoassays and 10 times more sensitive than conventional single radial immunodiffusion.", "contents": "Microfluorometric immunoassays with antigens bound to sepharose beads. Sepharose beads, to which protein had been coupled, were used in the development of two types of microfluorometric immunoassays. The first is an inhibition assay: a fixed amount of antiserum was absorbed with different amounts of antigen. This was accomplished in both direct and indirect incubation schemes. The system has been studied with human IgG and mouse IgM as an example. The second is a competition assay: fluorescein-labeled ovalbumin competea with unlabeled unlabeled ovalbumin for the binding sites of a rabbit antiovalbumin. This antiovalbumin was not bound directly to Sepharose but to Sepharose-coupled ovalbumin. In the indirect fluorescence inhibition and the competition assay, the sensitivity levels were determined. They proved to be the same, namely, 25 ng. This means that these fluorescence immunoassays are 10 times less sensitive than conventional radioimmunoassays and 10 times more sensitive than conventional single radial immunodiffusion."}
{"id": "PMID:1101770", "title": "Immunofluorescence studies of a possible prethymic T-cell differentiation in congenitally athymic (nude) mice.", "content": "A rabbit antimouse brain theta reagent was made specific for cells of the T lineage by absorption in vivo in nude mice. When used in double fluorescence together with an antimouse immunoglobulin reagent, four types of cells were found in spleen and lymph nodes of both normal and nude mice: Ig+thetaBr-, Ig-thetaBr+, Ig-thetaBr-, and Ig+thetaBr+. The data show that about 20% of nude mouse spleen lymphocytes are definitely of T lineage (Ig-thetaBr+). On these cells, the detection of the \"thetaBr\" determinant, which is identical or very close to the \"theta\" determinant, depends on the large amplification produced by indirect immunofluorescence, which suggests a low density of theta antigen. Similar experiments suggest the presence of cells that express some TL antigen in the spleen of nudes made congenic to a TL+ strain (BALB/c). It is proposed that the T-cell precursor that will further differentiate in the thymus already expresses a low density of theta and, in TL+ strains, TL antigen.", "contents": "Immunofluorescence studies of a possible prethymic T-cell differentiation in congenitally athymic (nude) mice. A rabbit antimouse brain theta reagent was made specific for cells of the T lineage by absorption in vivo in nude mice. When used in double fluorescence together with an antimouse immunoglobulin reagent, four types of cells were found in spleen and lymph nodes of both normal and nude mice: Ig+thetaBr-, Ig-thetaBr+, Ig-thetaBr-, and Ig+thetaBr+. The data show that about 20% of nude mouse spleen lymphocytes are definitely of T lineage (Ig-thetaBr+). On these cells, the detection of the \"thetaBr\" determinant, which is identical or very close to the \"theta\" determinant, depends on the large amplification produced by indirect immunofluorescence, which suggests a low density of theta antigen. Similar experiments suggest the presence of cells that express some TL antigen in the spleen of nudes made congenic to a TL+ strain (BALB/c). It is proposed that the T-cell precursor that will further differentiate in the thymus already expresses a low density of theta and, in TL+ strains, TL antigen."}
{"id": "PMID:1101773", "title": "Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection.", "content": "To detect antibodies to T. Spiralis in sera, the IF methods with the cuticle of T. spiralis larvae (the tube test) was compared to the cryostat method. In the latter method, cryostat sections were prepared from isolated T. spiralis larvae or from tongue or diaphragm musculature in which encysted T. spiralis larvae were present. In this case, both cuticle and internal structures were employed as antigenic sites. The cryostat method proved to be more sensitive than the tube test. With the cryostat method, specific antibodies were detected in sera of experimentally infected mice 14 days after infection, whereas with the tube test, antibodies were detected on Day 24 postinfection and consistently thereafter. The enzyme-linked immunosorbent assay (ELISA) was then studied. Quantitation of specific antibodies was achieved with alkaline phosphatase- or peroxidase-labeled antispecies immunoglobulin in antigen-coated tubes. The enzyme that remained in the tube after washing provided a measure of the amount of specific antibodies in the serum. A saline extract of T. spiralis larvae served as the antigen. In the experimental models studied (T. spiralis-infected rabbits and pigs), ELISA proved to be more sensitive than IF. At Day 3 postinfection and thereafter, specific antibodies could be detected. ELISA was modified to satisfy requirements for routine application.", "contents": "Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection. To detect antibodies to T. Spiralis in sera, the IF methods with the cuticle of T. spiralis larvae (the tube test) was compared to the cryostat method. In the latter method, cryostat sections were prepared from isolated T. spiralis larvae or from tongue or diaphragm musculature in which encysted T. spiralis larvae were present. In this case, both cuticle and internal structures were employed as antigenic sites. The cryostat method proved to be more sensitive than the tube test. With the cryostat method, specific antibodies were detected in sera of experimentally infected mice 14 days after infection, whereas with the tube test, antibodies were detected on Day 24 postinfection and consistently thereafter. The enzyme-linked immunosorbent assay (ELISA) was then studied. Quantitation of specific antibodies was achieved with alkaline phosphatase- or peroxidase-labeled antispecies immunoglobulin in antigen-coated tubes. The enzyme that remained in the tube after washing provided a measure of the amount of specific antibodies in the serum. A saline extract of T. spiralis larvae served as the antigen. In the experimental models studied (T. spiralis-infected rabbits and pigs), ELISA proved to be more sensitive than IF. At Day 3 postinfection and thereafter, specific antibodies could be detected. ELISA was modified to satisfy requirements for routine application."}
{"id": "PMID:1101774", "title": "Antibody-induced movement of protozoan surface membrane antigens.", "content": "Direct and indirect immunofluorescence techniques were used to study the effects of host antibodies on surface membrane antigens of viable Leishmania parasites in vitro. Antisera to L. enriettii and L. tropica caused surface membrane antigens of these parasites to aggregate, move toward the poles of the parasite, and to eventually disappear. This sequence did not occur at low temperature or in the presence of some metabolic inhibitors. Antigen subsequently reappeared on the parasite membrane. These phenomena appear to be similar to those described in mammalian cells. Antibodies may therefore effect an alteration in the surface membrane structure of living protozoan parasites.", "contents": "Antibody-induced movement of protozoan surface membrane antigens. Direct and indirect immunofluorescence techniques were used to study the effects of host antibodies on surface membrane antigens of viable Leishmania parasites in vitro. Antisera to L. enriettii and L. tropica caused surface membrane antigens of these parasites to aggregate, move toward the poles of the parasite, and to eventually disappear. This sequence did not occur at low temperature or in the presence of some metabolic inhibitors. Antigen subsequently reappeared on the parasite membrane. These phenomena appear to be similar to those described in mammalian cells. Antibodies may therefore effect an alteration in the surface membrane structure of living protozoan parasites."}
{"id": "PMID:1101778", "title": "Immunoglobulin responses after rubella infection.", "content": "The indirect immunofluorescence technique has been used to study specific IgG, IgA, and IgM antibodies in adults with acute rubella, volunteers who receive attentuated rubella vaccine, and infants with suspected congenital infection. In acute rubella, IgA and IgM antibodies reached peak titers during the second week after the rash and then declined, but specific IgG persisted. A similar pattern of response occurred after rubella vaccination, but titers were lower. Occasionally, fluorescent staining failed to detect specific IgM in whole serum but demonstrated it clearly in sucrose density gradient fractions. The improvement in fluorescence obtained by staining serum fractions was particularly striking in samples obtained from congenitally infected infants. In these cases, IgM staining with whole serum was often poor, but when fractions were tested, specific IgM was demonstrated in nearly all samples obtained within 9 months of birth. IgM antibody was seldom detected after the first year of life, but IgG antibody persisted. IgA antibody was not detected in congenital rubella.", "contents": "Immunoglobulin responses after rubella infection. The indirect immunofluorescence technique has been used to study specific IgG, IgA, and IgM antibodies in adults with acute rubella, volunteers who receive attentuated rubella vaccine, and infants with suspected congenital infection. In acute rubella, IgA and IgM antibodies reached peak titers during the second week after the rash and then declined, but specific IgG persisted. A similar pattern of response occurred after rubella vaccination, but titers were lower. Occasionally, fluorescent staining failed to detect specific IgM in whole serum but demonstrated it clearly in sucrose density gradient fractions. The improvement in fluorescence obtained by staining serum fractions was particularly striking in samples obtained from congenitally infected infants. In these cases, IgM staining with whole serum was often poor, but when fractions were tested, specific IgM was demonstrated in nearly all samples obtained within 9 months of birth. IgM antibody was seldom detected after the first year of life, but IgG antibody persisted. IgA antibody was not detected in congenital rubella."}
{"id": "PMID:1101780", "title": "A comparison between sorbent and Reiter sonicate in the absorbed fluorescent treponemal antibody test.", "content": "Some syphilitic sera still fluoresce with Reiter treponemes as the antigen after treatment with the sorbent used in the FTA-ABS test; this finding indicates incomplete removal of group antitreponemal antibody. Levels of this antibody in normal sera have been studied in relation to the titration of sorbent for use in the test. A comparison has been made of the results of FTA-ABS tests with sorbent and Reiter sonicate and the TPI test on 1141 selected problem sera. Results with the sonicate displayed closer agreement (60.9%) with those of the TPI test than did tests with sorbent 40.8%). The use of sonicate as the sorbing agent is thought to improve the performance of the FTA-ABS test without diminishing its sensitivity.", "contents": "A comparison between sorbent and Reiter sonicate in the absorbed fluorescent treponemal antibody test. Some syphilitic sera still fluoresce with Reiter treponemes as the antigen after treatment with the sorbent used in the FTA-ABS test; this finding indicates incomplete removal of group antitreponemal antibody. Levels of this antibody in normal sera have been studied in relation to the titration of sorbent for use in the test. A comparison has been made of the results of FTA-ABS tests with sorbent and Reiter sonicate and the TPI test on 1141 selected problem sera. Results with the sonicate displayed closer agreement (60.9%) with those of the TPI test than did tests with sorbent 40.8%). The use of sonicate as the sorbing agent is thought to improve the performance of the FTA-ABS test without diminishing its sensitivity."}
{"id": "PMID:1101782", "title": "Surface antigens on cultured malignant melanoma cells as detected by a membrane immunofluorescence method with human sera. Lack of tumor-specific reactions on melanoma lines.", "content": "Surface antigens on melanoma cells were studied with an MIF method applied for monolayers of cultured cells. Human sera were used with indirect immunofluorescence. Preparations were fixed in methanol after staining. Reactions of sera with antibodies against trasplantation antigens displayed a  fine granular fluorescence. This appearance was abolished by absorptions with pooled tonsilar lymphocytes. Melanoma patients' sera free from antibodies against transplantation antigens exhibited a coarse patchy fluorescence reaction. Melanoma patients' sera with antibodies against transplantation antigens yielded the second type of MIF reaction after absorptions with normal lymphocytes whereas further absorptions with melanoma line suspensions effectively removed all antibody reactivity...", "contents": "Surface antigens on cultured malignant melanoma cells as detected by a membrane immunofluorescence method with human sera. Lack of tumor-specific reactions on melanoma lines. Surface antigens on melanoma cells were studied with an MIF method applied for monolayers of cultured cells. Human sera were used with indirect immunofluorescence. Preparations were fixed in methanol after staining. Reactions of sera with antibodies against trasplantation antigens displayed a  fine granular fluorescence. This appearance was abolished by absorptions with pooled tonsilar lymphocytes. Melanoma patients' sera free from antibodies against transplantation antigens exhibited a coarse patchy fluorescence reaction. Melanoma patients' sera with antibodies against transplantation antigens yielded the second type of MIF reaction after absorptions with normal lymphocytes whereas further absorptions with melanoma line suspensions effectively removed all antibody reactivity..."}
{"id": "PMID:1101783", "title": "Studies on defined immunofluorescence in clinical immunopathology. III. Past progress, some new methods, and prospects for the future.", "content": "Experience with chessboard titrations over the past 9 years has shown that essentially all indirect IF staining systems that employ anti-IgG conjugates can be defined with the aid of assays for F/P ratios and/or antibody concentrations. Some improvements in the radial diffusion assays for antibody protein have been effected by refinements in methodology. Studies with a form of the Dass system showed that over a 16-fold range of light intensities, relatively little change occurred in \"titers\" of IgG (comparably to titers of patient sera) or in PEP conjugates as seen in DASS chessborad titrations. An analysis of 233 PEP values included in 12 literature reports on indirect IF tests indicate that 88% fall within one doubling dilution of 1/16 unit/ml or about 12.5 mug labeled Ab/ml. The DASS reactions also are within this range. Factors responsible for variation beyond these limits in indirect IF tests were analyzed. The most important factor appears to be experimental errors. Other factors include low titers of primary antibodies, some aberrant conjugates, and a recent shift toward greater sensitivity of chessboard titrations. The latter appears to be due primarily to modes of reading and should be controlled by the constant use of reference sera. It now seems possibe that future IF studies may be defined with the aid of this DASS system. To achieve this goal, it will be necessary to not only elaborate microanalytic techniques of the type that have been considered at this Conference but also to generate macroanalytic methods that can be used in parallel with them as a basis for interpreting microscopic observations both in the DASS system and in IF studies of sera and tissues.", "contents": "Studies on defined immunofluorescence in clinical immunopathology. III. Past progress, some new methods, and prospects for the future. Experience with chessboard titrations over the past 9 years has shown that essentially all indirect IF staining systems that employ anti-IgG conjugates can be defined with the aid of assays for F/P ratios and/or antibody concentrations. Some improvements in the radial diffusion assays for antibody protein have been effected by refinements in methodology. Studies with a form of the Dass system showed that over a 16-fold range of light intensities, relatively little change occurred in \"titers\" of IgG (comparably to titers of patient sera) or in PEP conjugates as seen in DASS chessborad titrations. An analysis of 233 PEP values included in 12 literature reports on indirect IF tests indicate that 88% fall within one doubling dilution of 1/16 unit/ml or about 12.5 mug labeled Ab/ml. The DASS reactions also are within this range. Factors responsible for variation beyond these limits in indirect IF tests were analyzed. The most important factor appears to be experimental errors. Other factors include low titers of primary antibodies, some aberrant conjugates, and a recent shift toward greater sensitivity of chessboard titrations. The latter appears to be due primarily to modes of reading and should be controlled by the constant use of reference sera. It now seems possibe that future IF studies may be defined with the aid of this DASS system. To achieve this goal, it will be necessary to not only elaborate microanalytic techniques of the type that have been considered at this Conference but also to generate macroanalytic methods that can be used in parallel with them as a basis for interpreting microscopic observations both in the DASS system and in IF studies of sera and tissues."}
{"id": "PMID:1101788", "title": "The specificity of fetal IgM: antibody or anti-antibody?", "content": "Reference materials were produced to standardize the immunoglobulin class specificity and potency of immunofluorescent anti-IgM conjugates used for diagnostic tests for congenital syphilis. In attempting to mimic essential immunologic characteristics of syphilitic and nonsyphilitic infant sera, we evaluated these sera in comparison with processed adult sera. We were quite surprised to discover that some syphilitic babies do not produce significant quantities to IgM antibody to T. pallidum in response to their infection, as would be expected; instead, they make relatively large amounts of IgM anti-IgG. We found this to be true also for newborns and infants infected with cytomegalovirus, rubella, and toxoplasmosis. To our knowledge, this observation has not been previously reported. However, it could have been predicted from the knowledge that older infants and young children normally produce IgM antibodies to maternal IgG allotypes (Gm factors). We are disturbed that these findings suggest that currently recommended indirect immunofluorescence IgM tests for perinatal infection may not be disease specific. Our observations may be important for a better understanding of basic immunologic mechanisms of fetal-maternal to tolerance and fetal response to life-threatening infection.", "contents": "The specificity of fetal IgM: antibody or anti-antibody? Reference materials were produced to standardize the immunoglobulin class specificity and potency of immunofluorescent anti-IgM conjugates used for diagnostic tests for congenital syphilis. In attempting to mimic essential immunologic characteristics of syphilitic and nonsyphilitic infant sera, we evaluated these sera in comparison with processed adult sera. We were quite surprised to discover that some syphilitic babies do not produce significant quantities to IgM antibody to T. pallidum in response to their infection, as would be expected; instead, they make relatively large amounts of IgM anti-IgG. We found this to be true also for newborns and infants infected with cytomegalovirus, rubella, and toxoplasmosis. To our knowledge, this observation has not been previously reported. However, it could have been predicted from the knowledge that older infants and young children normally produce IgM antibodies to maternal IgG allotypes (Gm factors). We are disturbed that these findings suggest that currently recommended indirect immunofluorescence IgM tests for perinatal infection may not be disease specific. Our observations may be important for a better understanding of basic immunologic mechanisms of fetal-maternal to tolerance and fetal response to life-threatening infection."}
{"id": "PMID:1101789", "title": "Ocular leprosy in Hawaii: the past.", "content": "Leprosy in Hawaii dates back to about 1840. The first recorded ocular leprosy is from the 1880s. Robert Louis Stevenson and Jack London both wrote descriptions of the ocular signs of leprosy. Pinkerton in 1927 and Van Poole in 1934 reported large series of patients with ocular leprosy. In 1973 there were about 2,168 cases of leprosy in the 50 states. Most ophthalmologists practice in areas where there are leprosy patients.", "contents": "Ocular leprosy in Hawaii: the past. Leprosy in Hawaii dates back to about 1840. The first recorded ocular leprosy is from the 1880s. Robert Louis Stevenson and Jack London both wrote descriptions of the ocular signs of leprosy. Pinkerton in 1927 and Van Poole in 1934 reported large series of patients with ocular leprosy. In 1973 there were about 2,168 cases of leprosy in the 50 states. Most ophthalmologists practice in areas where there are leprosy patients."}
{"id": "PMID:1101798", "title": "[Study of anti-macrophage serum and of its effect on antibody formation].", "content": "A specific anti-macrophage serum (AMS) has been obtained by immunizing rabbits with mouse peritoneal exudate cells and after repeated absorption with mouse erythrocytes, thymocytes and splenic lymphocytes. A comparative study of the cytotoxic activities of this serum on macrophages, thymocytes and lymph node cells, before and after absorption, showed that macrophage posses antigens which are common to both thymic and lymph node lymphocytes, and other antigens which are in common with lymph node lymphocytes but absent on thymocytes, as well as antigens being particular to macrophages. The absorbed sera had a definite cytotoxic effect on macrophages in vitro. This effect was observed when complement was added immediately or 4 hours after incubation with serum. It was lost after 24 hours. An inhibitory effects of AMS on phagocytosis in vivo has also been observed, which disappeared after one day. Antibody production in vivo was only moderately affected by a pretreatment with AMS. However, addition of AMS to spleen cell cultures stimulated with sheep red blood cells inhibited the appearance of hemolytic plaque forming cells.", "contents": "[Study of anti-macrophage serum and of its effect on antibody formation]. A specific anti-macrophage serum (AMS) has been obtained by immunizing rabbits with mouse peritoneal exudate cells and after repeated absorption with mouse erythrocytes, thymocytes and splenic lymphocytes. A comparative study of the cytotoxic activities of this serum on macrophages, thymocytes and lymph node cells, before and after absorption, showed that macrophage posses antigens which are common to both thymic and lymph node lymphocytes, and other antigens which are in common with lymph node lymphocytes but absent on thymocytes, as well as antigens being particular to macrophages. The absorbed sera had a definite cytotoxic effect on macrophages in vitro. This effect was observed when complement was added immediately or 4 hours after incubation with serum. It was lost after 24 hours. An inhibitory effects of AMS on phagocytosis in vivo has also been observed, which disappeared after one day. Antibody production in vivo was only moderately affected by a pretreatment with AMS. However, addition of AMS to spleen cell cultures stimulated with sheep red blood cells inhibited the appearance of hemolytic plaque forming cells."}
{"id": "PMID:1101799", "title": "The influence of cyclophosphamide on antibody formation in the mouse.", "content": "A single injection of cyclophosphamide (CY) (300 mg/kg) 8 h prior to immunization results in a complete suppression of the antibody formation in mice. The effect of CY on the number and activity of the surviving cells in the lymphoid organs was studied. For all organs tested there was a decrease in cell numbers to 10% or less till day 3, followed by an increase in cell numbers in bone marrow, spleen and lymph nodes. The increase in cell numbers in the thymus was delayed till after day 11. The bone marrow reached normal levels on day 7. The spleen surpassed the normal levels on day 11, reaching cell numbers which were 50% over the normal ones. For the thymus cells this was found only on day 21. The numbers of lymph node cells remained low and reached normal levels or higher only when the mice were stimulated after CY treatment. This effect of stimulation was further seen in the spleen cell numbers, where after stimulation with antigen in Freund's complete adjuvant (FCA) or only FCA cell numbers of 200% of normal values were obtained. The suppression of antibody formation by CY could be restored by cells from spleen, lymph nodes, Peyer's patches or thymus and bone marrow, indicating a defect in both B and T cells caused by CY treatment. The target cell of CY treatment was studied by reconstitution of anergic mice with spleen or lymph node cells of CY treated mice with or without addition of normal bone marrow and thymus cells. This revealed that although the B cells is the main target cell of CY action, the impact on the T cell population is also considerable. When however CY treatment is followed by antigenic stimulation, there is a steep increase in the activity of the lymph node cells when bone marrow cells are added. This phenomenon resembles the education of thymus cells in the spleen of lethally irradiated mice in the presence of antigen.", "contents": "The influence of cyclophosphamide on antibody formation in the mouse. A single injection of cyclophosphamide (CY) (300 mg/kg) 8 h prior to immunization results in a complete suppression of the antibody formation in mice. The effect of CY on the number and activity of the surviving cells in the lymphoid organs was studied. For all organs tested there was a decrease in cell numbers to 10% or less till day 3, followed by an increase in cell numbers in bone marrow, spleen and lymph nodes. The increase in cell numbers in the thymus was delayed till after day 11. The bone marrow reached normal levels on day 7. The spleen surpassed the normal levels on day 11, reaching cell numbers which were 50% over the normal ones. For the thymus cells this was found only on day 21. The numbers of lymph node cells remained low and reached normal levels or higher only when the mice were stimulated after CY treatment. This effect of stimulation was further seen in the spleen cell numbers, where after stimulation with antigen in Freund's complete adjuvant (FCA) or only FCA cell numbers of 200% of normal values were obtained. The suppression of antibody formation by CY could be restored by cells from spleen, lymph nodes, Peyer's patches or thymus and bone marrow, indicating a defect in both B and T cells caused by CY treatment. The target cell of CY treatment was studied by reconstitution of anergic mice with spleen or lymph node cells of CY treated mice with or without addition of normal bone marrow and thymus cells. This revealed that although the B cells is the main target cell of CY action, the impact on the T cell population is also considerable. When however CY treatment is followed by antigenic stimulation, there is a steep increase in the activity of the lymph node cells when bone marrow cells are added. This phenomenon resembles the education of thymus cells in the spleen of lethally irradiated mice in the presence of antigen."}
{"id": "PMID:1101800", "title": "[Immunological activities of rat lymphocytes. III. --Isolation of differing subpopulations of lymphocytes by two techniques of T cell separation].", "content": "In previous studies we have isolated by density gradient separation a population of relatively dense T lymphocytes capable of inhibiting the mitogenic response to concanavalin A (ConA) and phytohaemagglutinin. In this study we compared two current methods of cell separation for their relative yield in T lymphocytes able or not to respond to Con A. The present results show that cells passed through nylon wool columns--in contrast to those obtained by removal of lymphocytes which bind erythrocyte-antibody-complement complex-partially lose their ability to respond to ConA. The response lost by passage through nylon wool columns can be restored by centrifugation of the T cell suspension on a gradient of \"Ficoll-Hypaque\". This suggests that methods of T cells purification using density gradient separation may remove the more dense suppressor cells.", "contents": "[Immunological activities of rat lymphocytes. III. --Isolation of differing subpopulations of lymphocytes by two techniques of T cell separation]. In previous studies we have isolated by density gradient separation a population of relatively dense T lymphocytes capable of inhibiting the mitogenic response to concanavalin A (ConA) and phytohaemagglutinin. In this study we compared two current methods of cell separation for their relative yield in T lymphocytes able or not to respond to Con A. The present results show that cells passed through nylon wool columns--in contrast to those obtained by removal of lymphocytes which bind erythrocyte-antibody-complement complex-partially lose their ability to respond to ConA. The response lost by passage through nylon wool columns can be restored by centrifugation of the T cell suspension on a gradient of \"Ficoll-Hypaque\". This suggests that methods of T cells purification using density gradient separation may remove the more dense suppressor cells."}
{"id": "PMID:1101801", "title": "[Protective effects of bacterial immunostimulants in mice infected by \"Klebsiella pneumoniae\" resistant to antibiotics after mutation or by plasmid transfer].", "content": "A streptomycin-resistant strain of K. pneumoniae obtained after mutation in vitro was found to be less virulent than the sensitive strain in mice. However, a single injection of lipopolysaccharides (LPS) administered 24 hours before challenge increased the host's resistance to both strains. In contrast, the virulence was not changed in K. pneumoniae accepting R-factors for ampicillin, streptomycin, chloramphenicol, tetracycline and sulphonamide. The plasmids were transferred to K. pneumoniae from two strains of Escherichia coli possessing different R-factors with the same resistance pattern. As in the first case, mice pretreated by endotoxin were protected against a challenge by microorganisms carrying R-factors. The capacity of the stimulated host to destory resistant or sensitive organisms was of the same order. Klebsiella recovered 5 or 24 hours after infection from the blood, liver and spleen did not lost their antibiotic-resistance. In this study, BCG and Corynebacterium granulosum were also used. Like LPS, these two immunostimulants protected very effectively mice infected with K. pneumoniae rendered resistant to antibiotics by R-factor transfer.", "contents": "[Protective effects of bacterial immunostimulants in mice infected by \"Klebsiella pneumoniae\" resistant to antibiotics after mutation or by plasmid transfer]. A streptomycin-resistant strain of K. pneumoniae obtained after mutation in vitro was found to be less virulent than the sensitive strain in mice. However, a single injection of lipopolysaccharides (LPS) administered 24 hours before challenge increased the host's resistance to both strains. In contrast, the virulence was not changed in K. pneumoniae accepting R-factors for ampicillin, streptomycin, chloramphenicol, tetracycline and sulphonamide. The plasmids were transferred to K. pneumoniae from two strains of Escherichia coli possessing different R-factors with the same resistance pattern. As in the first case, mice pretreated by endotoxin were protected against a challenge by microorganisms carrying R-factors. The capacity of the stimulated host to destory resistant or sensitive organisms was of the same order. Klebsiella recovered 5 or 24 hours after infection from the blood, liver and spleen did not lost their antibiotic-resistance. In this study, BCG and Corynebacterium granulosum were also used. Like LPS, these two immunostimulants protected very effectively mice infected with K. pneumoniae rendered resistant to antibiotics by R-factor transfer."}
{"id": "PMID:1101797", "title": "Origin of the osseous bioelectric potentials: a review.", "content": "Recently, an electrophysical approach has been applied to the study of the long bone in order that a more complete understanding of its growth and structural integrity become available. Experimental studies have indicated that weak electric fields may be detected in living bone, in vivo, as the result of externally applied loads, normal weight bearing and pulsatile blood flow. In addition, physiological information may be derived from these electropotentials as functions of their magnitude and polarity. Several sources of these natural osseous bioelectric potentials have been proposed including piezoelectricity, electrokinetic phenomena, p-n semi-conductor junctions, pyroelectricity and photoelectricity. In this paper, these sourcesof bioelectricity are examined, their origins elucidated and their importance ranked in accordance with the maintenance of the structural integrity of the bone.", "contents": "Origin of the osseous bioelectric potentials: a review. Recently, an electrophysical approach has been applied to the study of the long bone in order that a more complete understanding of its growth and structural integrity become available. Experimental studies have indicated that weak electric fields may be detected in living bone, in vivo, as the result of externally applied loads, normal weight bearing and pulsatile blood flow. In addition, physiological information may be derived from these electropotentials as functions of their magnitude and polarity. Several sources of these natural osseous bioelectric potentials have been proposed including piezoelectricity, electrokinetic phenomena, p-n semi-conductor junctions, pyroelectricity and photoelectricity. In this paper, these sourcesof bioelectricity are examined, their origins elucidated and their importance ranked in accordance with the maintenance of the structural integrity of the bone."}
{"id": "PMID:1101809", "title": "[Sensitivity of bacterial flora to antibiotics according to data from a clinical hospital].", "content": "Antibiotic sensitivity of E. coli O-124, Salmonella and Shigella sonnei isolated from patients during 1968-1972 was studied with the disc method. Increase in the resistance levels to monomycin and neomycin in E. coli O-124 was observed. Sensitivity of Salmonella and Shigella sonnei to some antibiotics did not practically change.", "contents": "[Sensitivity of bacterial flora to antibiotics according to data from a clinical hospital]. Antibiotic sensitivity of E. coli O-124, Salmonella and Shigella sonnei isolated from patients during 1968-1972 was studied with the disc method. Increase in the resistance levels to monomycin and neomycin in E. coli O-124 was observed. Sensitivity of Salmonella and Shigella sonnei to some antibiotics did not practically change."}
{"id": "PMID:1101810", "title": "[Transmissivity of drug resistance in Salmonella].", "content": "The experiments showed that among 110 Salmonella strains resistant to 1 or more antibiotics 54.5 per cent of the cultures transfered their resistance to E. coli when grown in vitro. The frequency of the transmissive drug resistance in Salmonella was directly proportional to the number of r-determinants in R-factor. The cultures resistant to ampicillin and kanamycin proved to be the most competent donors.", "contents": "[Transmissivity of drug resistance in Salmonella]. The experiments showed that among 110 Salmonella strains resistant to 1 or more antibiotics 54.5 per cent of the cultures transfered their resistance to E. coli when grown in vitro. The frequency of the transmissive drug resistance in Salmonella was directly proportional to the number of r-determinants in R-factor. The cultures resistant to ampicillin and kanamycin proved to be the most competent donors."}
{"id": "PMID:1101811", "title": "[Study of the mechanisms of levomycetin inactivation by the palgue causative agent and Escherichia coli with episomal and chromosomal resistance. The enzymatic acetylation of levomycetin].", "content": "One of the mechanisms of levomycetin inactivation, i.e. enzymatic acetylation of the plague causative agent and Coli bacteria with chromosomic and episomic type of resistance was studied. It was shown that resistance to levomycetin in the recombinants of the plague microbe and Coli bacteria stipulated by one and the same R-factor was associated with their capacity for the antibiotic inactivation mainly with the help of levomycetinacetyltransferase. At the same time the enzyme activity in the mutants was very low and in the cells of Y. pestis and E. coli sensitive to the antibiotic it was absent. Levomycetinacetyltransferase is to somee extent soluble, still a singificant amount of it is connected with the structures of the bacterial cells and in particular with the cytoplasmic membranes. Comparison of the activity of levomycetinacetyltransferase as dependent on the incubation time, substrate concentration, pH and thermal treatment provided determination of quantitative differences in the properties of the enzyme of Y. petis and E. coli with episomic multiple drug resistance. However, under the experimental conditions transfer of R-factor from one host to the other induced no qualitative changes in the mechanism of levomycetin inactivation.", "contents": "[Study of the mechanisms of levomycetin inactivation by the palgue causative agent and Escherichia coli with episomal and chromosomal resistance. The enzymatic acetylation of levomycetin]. One of the mechanisms of levomycetin inactivation, i.e. enzymatic acetylation of the plague causative agent and Coli bacteria with chromosomic and episomic type of resistance was studied. It was shown that resistance to levomycetin in the recombinants of the plague microbe and Coli bacteria stipulated by one and the same R-factor was associated with their capacity for the antibiotic inactivation mainly with the help of levomycetinacetyltransferase. At the same time the enzyme activity in the mutants was very low and in the cells of Y. pestis and E. coli sensitive to the antibiotic it was absent. Levomycetinacetyltransferase is to somee extent soluble, still a singificant amount of it is connected with the structures of the bacterial cells and in particular with the cytoplasmic membranes. Comparison of the activity of levomycetinacetyltransferase as dependent on the incubation time, substrate concentration, pH and thermal treatment provided determination of quantitative differences in the properties of the enzyme of Y. petis and E. coli with episomic multiple drug resistance. However, under the experimental conditions transfer of R-factor from one host to the other induced no qualitative changes in the mechanism of levomycetin inactivation."}
{"id": "PMID:1101812", "title": "[First phase in the clinical study of the antineoplastic antibiotic, carminomycin].", "content": "During the 1st stage of the clinical trials of karminomycin 92 patients with leukemia, solid tumors and lymphoma were treated with karminomycin. Two schemes for the antibiotic use were developed. The 1st scheme was a prolonged one with single doses of 10-15 mg (7.5 mg/m2) administered intravenously twice a week for 3 weeks, the course dose being 60-75 mg (34-45 mg/m2) with 4-week intervals between the courses. The course dose for the patients previously subjected to intensive chemotherapy did not exceed 50 mg (30 mg/m2). The 2nd scheme was a short one with single doses of 8-10 mg (5.5 mg/m2) administered intravenously every day for 5 days, the course dose being 40-50 mg (23-30 mg/m2) with 3-week intervals between the courses. Karminomycin induced in a number of patients a direct side effect, such as nausea, vomiting, asthenia, tachycardia, pain in the heart. In some patients leucopenia, thrombocitopenia, rare stomatitis, alopecia, lowered T peak in the chest curves of the cardiograms were observed after using the course dose.", "contents": "[First phase in the clinical study of the antineoplastic antibiotic, carminomycin]. During the 1st stage of the clinical trials of karminomycin 92 patients with leukemia, solid tumors and lymphoma were treated with karminomycin. Two schemes for the antibiotic use were developed. The 1st scheme was a prolonged one with single doses of 10-15 mg (7.5 mg/m2) administered intravenously twice a week for 3 weeks, the course dose being 60-75 mg (34-45 mg/m2) with 4-week intervals between the courses. The course dose for the patients previously subjected to intensive chemotherapy did not exceed 50 mg (30 mg/m2). The 2nd scheme was a short one with single doses of 8-10 mg (5.5 mg/m2) administered intravenously every day for 5 days, the course dose being 40-50 mg (23-30 mg/m2) with 3-week intervals between the courses. Karminomycin induced in a number of patients a direct side effect, such as nausea, vomiting, asthenia, tachycardia, pain in the heart. In some patients leucopenia, thrombocitopenia, rare stomatitis, alopecia, lowered T peak in the chest curves of the cardiograms were observed after using the course dose."}
{"id": "PMID:1101813", "title": "Potential for in vivo acquisition of R plasmids by one strain of Vibrio cholerae biotype El tor.", "content": "The feces of five patients admitted to a hospital during an outbreak of cholera in Melbourne, Australia, in November 1972, were examined for the presence of tetracycline-resistant coliforms and tetracycline-resistant strains of Vibrio cholerae. Despite the abundance of tetracycline-resistant coliforms able to transfer this resistance to other strains of Escherichia coli, no tetracycline-resistant strains of V. cholerae were detected. In vitro transfer experiments using the V. cholerae strain responsible for the outbreak as recipient revealed that it was a particularly poor host for most R plasmids.", "contents": "Potential for in vivo acquisition of R plasmids by one strain of Vibrio cholerae biotype El tor. The feces of five patients admitted to a hospital during an outbreak of cholera in Melbourne, Australia, in November 1972, were examined for the presence of tetracycline-resistant coliforms and tetracycline-resistant strains of Vibrio cholerae. Despite the abundance of tetracycline-resistant coliforms able to transfer this resistance to other strains of Escherichia coli, no tetracycline-resistant strains of V. cholerae were detected. In vitro transfer experiments using the V. cholerae strain responsible for the outbreak as recipient revealed that it was a particularly poor host for most R plasmids."}
{"id": "PMID:1101814", "title": "In vitro studies with combinations of 5-fluorocytosine and amphotericin B.", "content": "Synergistic antifungal activity of 5-fluorocytosine (5-FC) and amphotericin B was studied using an abbreviated checkerboard titration scheme. 5-FC was titrated in twofold increments (100 to 0.05 mug/ml) in the absence and presence of graded increments of amphotericin B (1.0. 0.5, 0.1, 0.05, and 0.01 mug/ml) in buffered yeast nitrogen base. A limited number of experiments were performed using expanded dual titration checkerboard schemes and growth curve studies. Forty-eight isolates of yeastlike organisms were tested; two were inhibited by the buffer system. Evidence of synergy, as indicated by a fourfold or greater reduction of the minimal inhibitory concentration of 5-FC in the presence of subinhibitory concentrations of amphotericin B, was seen with 11 of 46 isolates, or 24%, at the fungistatic level and with three isolates, or 7% at the fungicidal level. Indifferent results were obtained for 44 and 74% of the isolates, respectively, at the fungistatic and fungicidal levels. Antagonism was observed with three isolates.", "contents": "In vitro studies with combinations of 5-fluorocytosine and amphotericin B. Synergistic antifungal activity of 5-fluorocytosine (5-FC) and amphotericin B was studied using an abbreviated checkerboard titration scheme. 5-FC was titrated in twofold increments (100 to 0.05 mug/ml) in the absence and presence of graded increments of amphotericin B (1.0. 0.5, 0.1, 0.05, and 0.01 mug/ml) in buffered yeast nitrogen base. A limited number of experiments were performed using expanded dual titration checkerboard schemes and growth curve studies. Forty-eight isolates of yeastlike organisms were tested; two were inhibited by the buffer system. Evidence of synergy, as indicated by a fourfold or greater reduction of the minimal inhibitory concentration of 5-FC in the presence of subinhibitory concentrations of amphotericin B, was seen with 11 of 46 isolates, or 24%, at the fungistatic level and with three isolates, or 7% at the fungicidal level. Indifferent results were obtained for 44 and 74% of the isolates, respectively, at the fungistatic and fungicidal levels. Antagonism was observed with three isolates."}
{"id": "PMID:1101815", "title": "Distribution of R plasmids among the O-antigen types of Escherichia coli isolated from human and animal sources.", "content": "The O-antigen types of 600 independently isolated Escherichia coli strains from human feces have been determined, and the types have been related to the antibiotic resistance patterns of the strains. The relative abundance of each O-antigen type differed in the susceptible and resistant series of strains. The majority (86%) of the resistant strains carried R plasmids. Resistant E. coli (20.3%) were found associated with O-antigen types 8, 9 and 101, whereas the susceptible strains covered a wide range of O-antigen types. Examination of 174 resistant strains isolated from calf feces also showed a prevalence of O-antigen types 8, 9, 101 (24.1%), and it seems probable that strains expressing these three O-antigen types commonly carry R plasmids in the alimentary tracts of man and calves. The number of strains not typeable with the O sera available were similar in the human (12.5%) and the calf (11.5%) series. There are no grounds for distinguishing \"human\" from \"calf\" E. coli on the basis of their O-antigen reactions.", "contents": "Distribution of R plasmids among the O-antigen types of Escherichia coli isolated from human and animal sources. The O-antigen types of 600 independently isolated Escherichia coli strains from human feces have been determined, and the types have been related to the antibiotic resistance patterns of the strains. The relative abundance of each O-antigen type differed in the susceptible and resistant series of strains. The majority (86%) of the resistant strains carried R plasmids. Resistant E. coli (20.3%) were found associated with O-antigen types 8, 9 and 101, whereas the susceptible strains covered a wide range of O-antigen types. Examination of 174 resistant strains isolated from calf feces also showed a prevalence of O-antigen types 8, 9, 101 (24.1%), and it seems probable that strains expressing these three O-antigen types commonly carry R plasmids in the alimentary tracts of man and calves. The number of strains not typeable with the O sera available were similar in the human (12.5%) and the calf (11.5%) series. There are no grounds for distinguishing \"human\" from \"calf\" E. coli on the basis of their O-antigen reactions."}
{"id": "PMID:1101816", "title": "Properties of the gentamicin acetyltransferase enzyme and application to the assay of aminoglycoside antibiotics.", "content": "The acetyltransferase technique of serum gentamicin assay was demonstrated to be specific for aminoglycoside antibiotics. The enzyme involved was isolated and stabilized by lyophilization.", "contents": "Properties of the gentamicin acetyltransferase enzyme and application to the assay of aminoglycoside antibiotics. The acetyltransferase technique of serum gentamicin assay was demonstrated to be specific for aminoglycoside antibiotics. The enzyme involved was isolated and stabilized by lyophilization."}
{"id": "PMID:1101817", "title": "Plasmid-determined epistatic susceptibility to kasugamycin.", "content": "The effect of two representative plasmids, R100 and F8-gal, on the susceptibility of Escherichia coli to kasugamycin was studied. R(+) and F(+) cells were found to be more susceptible to this antibiotic than R(-) and F(-) cells, respectively. Retransfer and curing experiments of these plasmids show that this increased susceptibility of host cells to kasugamycin was conferred by either of the plasmids. At the early stage of growth of R100(+) cells in the presence of kasugamycin, R(-) segregants overgrew the population and then they were replaced by kasugamycin-resistant mutants of the R(+) cells which became the majority cell line of the population. The former phenomenon is assumed to be due to the increased susceptibility of R100(+) cells to kasugamycin, and the latter is probably related to the finding that R100 enhances the spontaneous mutation of host cells to resistance to kasugamycin. The practical and experimental significance of these findings are discussed.", "contents": "Plasmid-determined epistatic susceptibility to kasugamycin. The effect of two representative plasmids, R100 and F8-gal, on the susceptibility of Escherichia coli to kasugamycin was studied. R(+) and F(+) cells were found to be more susceptible to this antibiotic than R(-) and F(-) cells, respectively. Retransfer and curing experiments of these plasmids show that this increased susceptibility of host cells to kasugamycin was conferred by either of the plasmids. At the early stage of growth of R100(+) cells in the presence of kasugamycin, R(-) segregants overgrew the population and then they were replaced by kasugamycin-resistant mutants of the R(+) cells which became the majority cell line of the population. The former phenomenon is assumed to be due to the increased susceptibility of R100(+) cells to kasugamycin, and the latter is probably related to the finding that R100 enhances the spontaneous mutation of host cells to resistance to kasugamycin. The practical and experimental significance of these findings are discussed."}
{"id": "PMID:1101818", "title": "Nalidixic acid: an antibacterial paradox.", "content": "Nalidixic acid was found to be most bactericidal against various species of gram-negative bacteria at 50 to 200 mug/ml. With all species tested, increases in the concentration of nalidixic acid above this range reduced, rather than increased, its bactericidal effect so that, at levels in the region of 400 mug/ml, the drug was relatively bacteriostatic. Therefore, the mode of action of nalidixic acid at various concentrations was investigated. It was found that at the most bactericidal concentration deoxyribonucleic acid synthesis, but no ribonucleic acid (RNA) or protein synthesis, was inhibited. However at higher concentrations, where the drug is least bactericidal, both RNA and protein synthesis were found to be inhibited. Results are presented which suggest that the protein synthesis inhibition is a secondary manifestation of the ability of the drug to inhibit RNA synthesis, and that of RNA synthesis is most likely the second target site for the action of the drug when bacteria are exposed to it in high concentrations. The clinical implications of these findings are discussed.", "contents": "Nalidixic acid: an antibacterial paradox. Nalidixic acid was found to be most bactericidal against various species of gram-negative bacteria at 50 to 200 mug/ml. With all species tested, increases in the concentration of nalidixic acid above this range reduced, rather than increased, its bactericidal effect so that, at levels in the region of 400 mug/ml, the drug was relatively bacteriostatic. Therefore, the mode of action of nalidixic acid at various concentrations was investigated. It was found that at the most bactericidal concentration deoxyribonucleic acid synthesis, but no ribonucleic acid (RNA) or protein synthesis, was inhibited. However at higher concentrations, where the drug is least bactericidal, both RNA and protein synthesis were found to be inhibited. Results are presented which suggest that the protein synthesis inhibition is a secondary manifestation of the ability of the drug to inhibit RNA synthesis, and that of RNA synthesis is most likely the second target site for the action of the drug when bacteria are exposed to it in high concentrations. The clinical implications of these findings are discussed."}
{"id": "PMID:1101819", "title": "Relation of beta-lactamase activity to antimicrobial susceptibility in Serratia marcescens.", "content": "One-hundred clinical isolates of Serratia marcescens were tested for susceptibility to cephalothin, carbenicillin, ticarcillin, ampicillin, and cefoxitin. The majority of the 100 isolates (>/=70%) were susceptible to carbenicillin, ticarcillin, and cefoxitin; less than one-half were susceptible to ampicillin; none were susceptible to cephalothin. Ten isolates from the 100 organisms tested were selectively assayed for their beta-lactamase activity. Enzyme activity was measured using either iodometric or spectrophotometric methods, and the microbiological assay technique. It was concluded that beta-lactamase production was not the sole determinant in beta-lactam antibiotic resistance. Resistance without demonstrable beta-lactamase was evident in strains for cephalothin, ampicillin, and cefoxitin. In addition, one strain which was susceptible to all antibiotics except cephalothin, elaborated considerable beta-lactamase activity.", "contents": "Relation of beta-lactamase activity to antimicrobial susceptibility in Serratia marcescens. One-hundred clinical isolates of Serratia marcescens were tested for susceptibility to cephalothin, carbenicillin, ticarcillin, ampicillin, and cefoxitin. The majority of the 100 isolates (>/=70%) were susceptible to carbenicillin, ticarcillin, and cefoxitin; less than one-half were susceptible to ampicillin; none were susceptible to cephalothin. Ten isolates from the 100 organisms tested were selectively assayed for their beta-lactamase activity. Enzyme activity was measured using either iodometric or spectrophotometric methods, and the microbiological assay technique. It was concluded that beta-lactamase production was not the sole determinant in beta-lactam antibiotic resistance. Resistance without demonstrable beta-lactamase was evident in strains for cephalothin, ampicillin, and cefoxitin. In addition, one strain which was susceptible to all antibiotics except cephalothin, elaborated considerable beta-lactamase activity."}
{"id": "PMID:1101820", "title": "Evaluation of topical polyinosinic acid-polycytidylic acid in treatment of localized herpes zoster in children with cancer: a randomized, double-blind controlled study.", "content": "Twenty-four children with cancer and localized herpes zoster were randomized to receive either topical polyinosinic acid-polycytidylic acid [poly(I:C)] or a saline placebo. Solutions containing 1.0 or 2.5 mg of drug per ml were applied to the lesions every 3 h, seven times a day for 7 consecutive days. Serial, standardized color photographs were used to evaluate the progression of lesions and maximum percentage of dermatome involvement. In patients receiving poly(I:C), the median days of new lesions after therapy was 2.0 days as opposed to 3.5 days in placebo-treated patients. This difference was not statistically significant. Moreover, the total median days of lesions and percentage of dermatome involvement were similar in both groups, as were complications. Neither the concentration nor the total dose of drug was related to the outcome of infection. We conclude that topical poly(I:C), as used in this study, was of no benefit in treatment of localized herpes zoster.", "contents": "Evaluation of topical polyinosinic acid-polycytidylic acid in treatment of localized herpes zoster in children with cancer: a randomized, double-blind controlled study. Twenty-four children with cancer and localized herpes zoster were randomized to receive either topical polyinosinic acid-polycytidylic acid [poly(I:C)] or a saline placebo. Solutions containing 1.0 or 2.5 mg of drug per ml were applied to the lesions every 3 h, seven times a day for 7 consecutive days. Serial, standardized color photographs were used to evaluate the progression of lesions and maximum percentage of dermatome involvement. In patients receiving poly(I:C), the median days of new lesions after therapy was 2.0 days as opposed to 3.5 days in placebo-treated patients. This difference was not statistically significant. Moreover, the total median days of lesions and percentage of dermatome involvement were similar in both groups, as were complications. Neither the concentration nor the total dose of drug was related to the outcome of infection. We conclude that topical poly(I:C), as used in this study, was of no benefit in treatment of localized herpes zoster."}
{"id": "PMID:1101821", "title": "Sequence effect on ampicillin blood levels noted in an amoxicillin, ampicillin, and epicillin triple crossover study.", "content": "Amoxicillin, ampicillin, and epicillin (500 mg of each) were given orally to fasting men and women in a triple crossover study. Peak serum concentrations were significantly higher for amoxicillin than for ampicillin and significantly lowest for epicillin. The concentrations of antibiotics in serum were comparable in men and women. Total urine recovery was highest for amoxicillin (56.7%) and lowest for epicillin (27.5%), and higher in men than in women for each of the three antibiotics. Saliva, sweat, and tears contained only very small amounts of amoxicillin and, rarely, ampicillin or epicillin. A significant (P < 0.02) sequence effect was noted in that peak serum concentrations of ampicillin were higher (6.4 mug/ml) if epicillin had been taken the previous week than when ampicillin was taken first (2.7 mug/ml).", "contents": "Sequence effect on ampicillin blood levels noted in an amoxicillin, ampicillin, and epicillin triple crossover study. Amoxicillin, ampicillin, and epicillin (500 mg of each) were given orally to fasting men and women in a triple crossover study. Peak serum concentrations were significantly higher for amoxicillin than for ampicillin and significantly lowest for epicillin. The concentrations of antibiotics in serum were comparable in men and women. Total urine recovery was highest for amoxicillin (56.7%) and lowest for epicillin (27.5%), and higher in men than in women for each of the three antibiotics. Saliva, sweat, and tears contained only very small amounts of amoxicillin and, rarely, ampicillin or epicillin. A significant (P < 0.02) sequence effect was noted in that peak serum concentrations of ampicillin were higher (6.4 mug/ml) if epicillin had been taken the previous week than when ampicillin was taken first (2.7 mug/ml)."}
{"id": "PMID:1101822", "title": "Feasibility of administering aminoglycoside antibiotics by continuous intravenous infusion.", "content": "Eleven patients each received gentamicin sulfate, tobramycin, and sisomicin by continuous intravenous infusion after an initial loading dose. Subsequent doses of antibiotic were adjusted in an attempt to maintain constant serum concentrations. There was considerable variation in the serum concentration from patient to patient and in the same patient from day to day with each drug. Although the dosages of gentamicin sulfate and tobramycin were similar, the serum concentrations of the latter drug were consistently lower. Despite the daily administration of doses of at least 300 mg of gentamicin and tobramycin per m(2) and 160 mg of sisomicin per m(2), nephrotoxicity occurred in only three patients. This is a low frequency of nephrotoxicity, considering the dosages of drug administered. Although therapeutic efficacy was not an objective of this study, 8 of 11 documented infections were cured. This approach to the administration of aminoglycoside antibiotics deserves therapeutic trials.", "contents": "Feasibility of administering aminoglycoside antibiotics by continuous intravenous infusion. Eleven patients each received gentamicin sulfate, tobramycin, and sisomicin by continuous intravenous infusion after an initial loading dose. Subsequent doses of antibiotic were adjusted in an attempt to maintain constant serum concentrations. There was considerable variation in the serum concentration from patient to patient and in the same patient from day to day with each drug. Although the dosages of gentamicin sulfate and tobramycin were similar, the serum concentrations of the latter drug were consistently lower. Despite the daily administration of doses of at least 300 mg of gentamicin and tobramycin per m(2) and 160 mg of sisomicin per m(2), nephrotoxicity occurred in only three patients. This is a low frequency of nephrotoxicity, considering the dosages of drug administered. Although therapeutic efficacy was not an objective of this study, 8 of 11 documented infections were cured. This approach to the administration of aminoglycoside antibiotics deserves therapeutic trials."}
{"id": "PMID:1101823", "title": "Effect of surfactants on antibiotic resistance.", "content": "The effectiveness of surfactants as potentiators of antibiotic activity on several resistant strains of bacteria, selected from clinical sources and laboratory collections, was studied using a tube dilution assay. Bacterial strains included members of the Enterobacteriaceae and staphylococci. Cetyltrimethylammonium bromide (CTAB), Tween 80 (Tw80), a mixture of n-alkyldimethyl betaines (L14), and alpha-(2,4,5-trichlorophenoxy) propionic acid (TCP) were tested in combination with pencillin G (PenG), methicillin (Met), streptomycin (Sm), polymyxin B (PmB), and chlortetracycline (CTC). Growth response to the drug combinations was compared with the response to each drug alone. CTAB and L14 but not Tw80 or TCP were found to potentiate the activity of CTC on strains of Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae. Studies on the inhibition of protein synthesis by CTC in cells of a strain of E. coli suggested that the surfactants increased the uptake of antibiotic into the cells. CTAB and L14 almost completely sensitized strains of P. mirabilis, Serratia marcescens, K. pneumoniae, and E. coli to PmB. With the exception of K. pneumoniae, TCP was also effective in potentiating the activity of PmB on the above strains whereas Tw80 showed potentiation only with a strain of E. coli. CTAB and L14 but not TCP or Tw80 potentiated the activity of PenG but not Met on strains of staphylococci. Studies of penicillinase in the cells suggested that the surfactants inhibited the formation of this enzyme possibly at the level of induction. None of the surfactants were found to potentiate the activity of Sm.", "contents": "Effect of surfactants on antibiotic resistance. The effectiveness of surfactants as potentiators of antibiotic activity on several resistant strains of bacteria, selected from clinical sources and laboratory collections, was studied using a tube dilution assay. Bacterial strains included members of the Enterobacteriaceae and staphylococci. Cetyltrimethylammonium bromide (CTAB), Tween 80 (Tw80), a mixture of n-alkyldimethyl betaines (L14), and alpha-(2,4,5-trichlorophenoxy) propionic acid (TCP) were tested in combination with pencillin G (PenG), methicillin (Met), streptomycin (Sm), polymyxin B (PmB), and chlortetracycline (CTC). Growth response to the drug combinations was compared with the response to each drug alone. CTAB and L14 but not Tw80 or TCP were found to potentiate the activity of CTC on strains of Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae. Studies on the inhibition of protein synthesis by CTC in cells of a strain of E. coli suggested that the surfactants increased the uptake of antibiotic into the cells. CTAB and L14 almost completely sensitized strains of P. mirabilis, Serratia marcescens, K. pneumoniae, and E. coli to PmB. With the exception of K. pneumoniae, TCP was also effective in potentiating the activity of PmB on the above strains whereas Tw80 showed potentiation only with a strain of E. coli. CTAB and L14 but not TCP or Tw80 potentiated the activity of PenG but not Met on strains of staphylococci. Studies of penicillinase in the cells suggested that the surfactants inhibited the formation of this enzyme possibly at the level of induction. None of the surfactants were found to potentiate the activity of Sm."}
{"id": "PMID:1101824", "title": "Binding of bacteriocin Clo DF13 to Clo DF13 plasmid deoxyribonucleic acid in vivo and in vitro.", "content": "The bacteriocinogenic plasmid Clo DF13 is present in Escherichia coli to the extent of 10 copies per cell. A complex of Clo DF13 plasmid deoxyribonucleic acid (DNA) and protein can be isolated from cells. Treatment of the complex with ionic detergents or proteases dissociates the complex but does not convert any supercoiled Clo DF13 DNA to the open circular form, indicating that this complex is not a relaxation complex. The complex is stable in 0.5 M NaCl and contains one polypeptide species. The protein, present in the complex, appeared to be bacteriocin Clo DF13 for the following reasons: (i) the protein is de novo synthesized in Clo DF13-harboring minicells, indicating that this protein is Clo DF13 specific; (ii) this protein shows bacteriocinogenic activity on a bacteriocin Clo DF13-susceptible indicator strain; (iii) this protein has the same molecular weight (60,000) as bacteriocin Clo DF13. DNA-protein binding experiments, involving QAE-Sephadex column chromatography and nitrocellulose membrane filters, demonstrate that bacteriocin Clo DF13 has also affinity in vitro for Clo DF13 DNA. Membrane filter binding experiments revealed that bacteriocin Clo DF13 does not interact with other DNA species, such as ColE1 DNA, yeast DNA, calf thymus DNA, phiX174 DNA, and also not with denatured Clo DF13 DNA. In addition no binding to Clo DF13 DNA of a related bacteriocin, colicin E3, could be detected. These results indicate that the binding of bacteriocin Clo DF 13 to double-stranded Clo DF13 DNA is very specific.", "contents": "Binding of bacteriocin Clo DF13 to Clo DF13 plasmid deoxyribonucleic acid in vivo and in vitro. The bacteriocinogenic plasmid Clo DF13 is present in Escherichia coli to the extent of 10 copies per cell. A complex of Clo DF13 plasmid deoxyribonucleic acid (DNA) and protein can be isolated from cells. Treatment of the complex with ionic detergents or proteases dissociates the complex but does not convert any supercoiled Clo DF13 DNA to the open circular form, indicating that this complex is not a relaxation complex. The complex is stable in 0.5 M NaCl and contains one polypeptide species. The protein, present in the complex, appeared to be bacteriocin Clo DF13 for the following reasons: (i) the protein is de novo synthesized in Clo DF13-harboring minicells, indicating that this protein is Clo DF13 specific; (ii) this protein shows bacteriocinogenic activity on a bacteriocin Clo DF13-susceptible indicator strain; (iii) this protein has the same molecular weight (60,000) as bacteriocin Clo DF13. DNA-protein binding experiments, involving QAE-Sephadex column chromatography and nitrocellulose membrane filters, demonstrate that bacteriocin Clo DF13 has also affinity in vitro for Clo DF13 DNA. Membrane filter binding experiments revealed that bacteriocin Clo DF13 does not interact with other DNA species, such as ColE1 DNA, yeast DNA, calf thymus DNA, phiX174 DNA, and also not with denatured Clo DF13 DNA. In addition no binding to Clo DF13 DNA of a related bacteriocin, colicin E3, could be detected. These results indicate that the binding of bacteriocin Clo DF 13 to double-stranded Clo DF13 DNA is very specific."}
{"id": "PMID:1101825", "title": "Enhancement by nalidixic acid of the thermal susceptibility of the Ts-7 mutant of Escherichia coli TAU-bar.", "content": "Nadilidixic acid at 5 x 10(-6) M produced a substantial increase in thermal susceptibility of Ts-7, suggesting either that the thermal and nalidixic acid targets are identical or closely interdependent.", "contents": "Enhancement by nalidixic acid of the thermal susceptibility of the Ts-7 mutant of Escherichia coli TAU-bar. Nadilidixic acid at 5 x 10(-6) M produced a substantial increase in thermal susceptibility of Ts-7, suggesting either that the thermal and nalidixic acid targets are identical or closely interdependent."}
{"id": "PMID:1101826", "title": "Stability of Escherichia coli membrane proteins during chloramphenicol treatment.", "content": "It is shown that there is no degradation of previously formed inner and outer membrane proteins of Escherichia coli during 1 h of chloramphenicol treatment.", "contents": "Stability of Escherichia coli membrane proteins during chloramphenicol treatment. It is shown that there is no degradation of previously formed inner and outer membrane proteins of Escherichia coli during 1 h of chloramphenicol treatment."}
{"id": "PMID:1101827", "title": "Detection of mannitol formation by bacteria.", "content": "A test is described by means of which formation of mannitol from fructose by lactic acid bacteria can be readily detected. The test is based on removal of interference of residual fructose by dehydration with hydrochloric acid followed by thin-layer chromatography.", "contents": "Detection of mannitol formation by bacteria. A test is described by means of which formation of mannitol from fructose by lactic acid bacteria can be readily detected. The test is based on removal of interference of residual fructose by dehydration with hydrochloric acid followed by thin-layer chromatography."}
{"id": "PMID:1101833", "title": "Immunocytochemical localization of cathepsin D in rabbit skin.", "content": "The lysosomal proteinase cathepsin D has been localized in rabbit skin by immunocytochemical techniques. The enzyme was found in the basal layer of the epidermis, hair follicles, sebaceous glands, fibroblasts, and endothelial cells. An autoradiographic technique, using the Fab' fragment of IgG, was also used for more effective identification of cell types that contain cathepsin D.", "contents": "Immunocytochemical localization of cathepsin D in rabbit skin. The lysosomal proteinase cathepsin D has been localized in rabbit skin by immunocytochemical techniques. The enzyme was found in the basal layer of the epidermis, hair follicles, sebaceous glands, fibroblasts, and endothelial cells. An autoradiographic technique, using the Fab' fragment of IgG, was also used for more effective identification of cell types that contain cathepsin D."}
{"id": "PMID:1101835", "title": "Congenital myotonic dystrophy in Britain. I. Clinical aspects.", "content": "A clinical and genetic study of congenital myotonic dystrophy in Britain has been carried out in 70 patients from 54 sibships. The clinical aspects are analysed here, and the existence of a syndrome clinically distinct from myotonic dystrophy of later onset is confirmed. Characteristic features included neonatal hypotonia, motor and mental retardation, and facial diplegia. A high incidence of talipes occurs at birth together with hydramnios and reduced fetal movements during pregnancy, factors suggesting prenatal onset of the disorder in many cases. Prolonged survival is the rule after infancy, but the occurrence of numerous neonatal deaths in the sibships suggests the existence of unrecognized cases dying in the neonatal period.", "contents": "Congenital myotonic dystrophy in Britain. I. Clinical aspects. A clinical and genetic study of congenital myotonic dystrophy in Britain has been carried out in 70 patients from 54 sibships. The clinical aspects are analysed here, and the existence of a syndrome clinically distinct from myotonic dystrophy of later onset is confirmed. Characteristic features included neonatal hypotonia, motor and mental retardation, and facial diplegia. A high incidence of talipes occurs at birth together with hydramnios and reduced fetal movements during pregnancy, factors suggesting prenatal onset of the disorder in many cases. Prolonged survival is the rule after infancy, but the occurrence of numerous neonatal deaths in the sibships suggests the existence of unrecognized cases dying in the neonatal period."}
{"id": "PMID:1101836", "title": "The pathogenesis of pulmonary edema in acute pancreatitis.", "content": "Acute pulmonary edema appeared 3 or more days after the onset of acute pancreatitis in 7 patients, an approximate incidence of 8%. The severity of pancreatitis in these patients was characterized by massive requirements for intravenous colloid and by marked hypocalcemia. In addition, at least 5 of the 7 patients had very high serum levels of triglycerides at the time of hospital admission. Hemodynamic studies during pulmonary edema showed normal central venous pressure, pulmonary artery pressure, pulmonary capillary wedge pressure, and pulmonary vascular resistance. Cardiac index was appropriately elevated. Respiratory treatment, consisting of endotracheal intubation and controlled ventilation with PEEP, was successful in allowing reversal of the pulmonary injury and recovery of respiratory function within 1-2 weeks in all cases. Two patients died later from pancreatic abscesses. The findings indicate that a distinct form of pulmonary injury may occur in acute pancreatitis, characterized by loss of integrity of the alveolar-capilllary membrane, leading to pulmonary edema. The mechanism of injury is not known but may be caused by circulating free fatty acids, phospholipase A, or vasoactive substances. The pulmonary membrane lesion appears to heal during the period of intensive respiratory support.", "contents": "The pathogenesis of pulmonary edema in acute pancreatitis. Acute pulmonary edema appeared 3 or more days after the onset of acute pancreatitis in 7 patients, an approximate incidence of 8%. The severity of pancreatitis in these patients was characterized by massive requirements for intravenous colloid and by marked hypocalcemia. In addition, at least 5 of the 7 patients had very high serum levels of triglycerides at the time of hospital admission. Hemodynamic studies during pulmonary edema showed normal central venous pressure, pulmonary artery pressure, pulmonary capillary wedge pressure, and pulmonary vascular resistance. Cardiac index was appropriately elevated. Respiratory treatment, consisting of endotracheal intubation and controlled ventilation with PEEP, was successful in allowing reversal of the pulmonary injury and recovery of respiratory function within 1-2 weeks in all cases. Two patients died later from pancreatic abscesses. The findings indicate that a distinct form of pulmonary injury may occur in acute pancreatitis, characterized by loss of integrity of the alveolar-capilllary membrane, leading to pulmonary edema. The mechanism of injury is not known but may be caused by circulating free fatty acids, phospholipase A, or vasoactive substances. The pulmonary membrane lesion appears to heal during the period of intensive respiratory support."}
{"id": "PMID:1101839", "title": "Hypothalamic and nutritional obesities in mice. A comparative study of growth and body composition.", "content": "Obesities induced in mice by a single injection of gold thioglucose or by a change to a fat-enriched diet(containing 8% fat) were studied in young and adult mice of Charles River strain. These two forms of obesity were compared and contrasted with normal animals maintained on 2.5% fat diet for life. The comparison was based on individual growth, age-realted fat, water and defatted dry weight of carcasses and their distribution of weights within larger populations. Young animals on diets containing 8% fat showed an elevated accumulation of fat but not of water. Individual variations lead to an abnormally distributed population where in some animals the excess in weight was characterized by an excessive fat and lean body mass and in others by an excess fat only. Some animals showed no excessive weight when compared to animals maintained on 2.5% fat. The rapid gain in weight which followed a successful injection with gold thioglucose showed a normal distrubution and was correlated with an accumulation of fat in young and adult animals. A significant retention of water was observed only in adults. The relative merits of these obesities as models for human regulatory obesities are briefly discussed.", "contents": "Hypothalamic and nutritional obesities in mice. A comparative study of growth and body composition. Obesities induced in mice by a single injection of gold thioglucose or by a change to a fat-enriched diet(containing 8% fat) were studied in young and adult mice of Charles River strain. These two forms of obesity were compared and contrasted with normal animals maintained on 2.5% fat diet for life. The comparison was based on individual growth, age-realted fat, water and defatted dry weight of carcasses and their distribution of weights within larger populations. Young animals on diets containing 8% fat showed an elevated accumulation of fat but not of water. Individual variations lead to an abnormally distributed population where in some animals the excess in weight was characterized by an excessive fat and lean body mass and in others by an excess fat only. Some animals showed no excessive weight when compared to animals maintained on 2.5% fat. The rapid gain in weight which followed a successful injection with gold thioglucose showed a normal distrubution and was correlated with an accumulation of fat in young and adult animals. A significant retention of water was observed only in adults. The relative merits of these obesities as models for human regulatory obesities are briefly discussed."}
{"id": "PMID:1101842", "title": "Very high dosage vs standard dosage fluphenazine in schizophrenia. A double-blind study of nonchronic treatment-refractory patients.", "content": "Previous work with chronic schizophrenic patients and a pilot study with nonchronic treatment-refractory schizophrenic patients indicated that very high doses of fluphenazine hydrochloride (1,200 mg/day) have a greater antipsychotic effect than do standard doses. Increased side-effects were not reported. In a double-blind six-week random assignment study, 18 nonchronic treatment-refractory patients received the very high dose and 13 the standard dose. The standard-dose treated patients had greater improvement on a variety of measures. Analysis of Inpatient Multidimensional Psychiatric Scale scores indicate that some patients taking very high doses had akinesia, and extrapyramidal side-effect that in part accounted for their inferior response.", "contents": "Very high dosage vs standard dosage fluphenazine in schizophrenia. A double-blind study of nonchronic treatment-refractory patients. Previous work with chronic schizophrenic patients and a pilot study with nonchronic treatment-refractory schizophrenic patients indicated that very high doses of fluphenazine hydrochloride (1,200 mg/day) have a greater antipsychotic effect than do standard doses. Increased side-effects were not reported. In a double-blind six-week random assignment study, 18 nonchronic treatment-refractory patients received the very high dose and 13 the standard dose. The standard-dose treated patients had greater improvement on a variety of measures. Analysis of Inpatient Multidimensional Psychiatric Scale scores indicate that some patients taking very high doses had akinesia, and extrapyramidal side-effect that in part accounted for their inferior response."}
{"id": "PMID:1101843", "title": "Amitriptyline-perphenazine interaction in ambulatory schizophrenic patients. A controled study of drug interaction.", "content": "In a double-blind placebo, controlled clinical study, lasting 12 weeks, 48 male and 48 female ambulatory schizophrenic patients were randomly assigned to one of four treatments: placebo; amitriptyline hydrochloride, 125 mg/day; perphenazine, 20 mg/day; or amitriptyline-perphenazine, 20 mg/day. Treatment groups contained an equal number of male and female patients. Perphenazine alone or in combination was substantially more effective in reducing psychopathological disorder than was the placebo, but there was no evidence to indicate the superiority of the amitriptyline-perphenazine combination over perphenazine alone. Amitriptyline alone was not substantially better than placebo and could not be considered an efficacious medication for the maintenance treatment of these patients. Less response to treatment was made by patients with longer-term records of prior hospitalization.", "contents": "Amitriptyline-perphenazine interaction in ambulatory schizophrenic patients. A controled study of drug interaction. In a double-blind placebo, controlled clinical study, lasting 12 weeks, 48 male and 48 female ambulatory schizophrenic patients were randomly assigned to one of four treatments: placebo; amitriptyline hydrochloride, 125 mg/day; perphenazine, 20 mg/day; or amitriptyline-perphenazine, 20 mg/day. Treatment groups contained an equal number of male and female patients. Perphenazine alone or in combination was substantially more effective in reducing psychopathological disorder than was the placebo, but there was no evidence to indicate the superiority of the amitriptyline-perphenazine combination over perphenazine alone. Amitriptyline alone was not substantially better than placebo and could not be considered an efficacious medication for the maintenance treatment of these patients. Less response to treatment was made by patients with longer-term records of prior hospitalization."}
{"id": "PMID:1101844", "title": "Comparison of efficacy of lithium carbonate and chlorpromazine in mania. Report of collaborative study group on treatment of mania in Japan.", "content": "A multi-institutional cooperative study comparing lithium carbonate with chlorpromazine was conducted, using a controlled double-blind design in a series of 80 cases of endogenous manic psychosis, to evaluate the drugs' clinical utility and efficacy, characteristics of therapeutic effect, and side-effects. Dosages employed were consistently at an equipotent ratio of 4:1 (lithium carbonate:chlorpromazine). Physicians' overall ratings showed lithium carbonate as significantly superior to chlorpromazine in efficacy for manic psychosis. Improvements of basic mood and of disturbance in speech and voice were prominent with lithium carbonate. Onset of the therapeutic effect of lithium carbonate was within ten days of medication in 65% of the patients, significantly faster than with chlorpromazine. Side-effects encountered with lithium carbonate therapy at dose levels not higher than 1,800 mg/day were milder and less frequent compared with those seen with chlorpromazine.", "contents": "Comparison of efficacy of lithium carbonate and chlorpromazine in mania. Report of collaborative study group on treatment of mania in Japan. A multi-institutional cooperative study comparing lithium carbonate with chlorpromazine was conducted, using a controlled double-blind design in a series of 80 cases of endogenous manic psychosis, to evaluate the drugs' clinical utility and efficacy, characteristics of therapeutic effect, and side-effects. Dosages employed were consistently at an equipotent ratio of 4:1 (lithium carbonate:chlorpromazine). Physicians' overall ratings showed lithium carbonate as significantly superior to chlorpromazine in efficacy for manic psychosis. Improvements of basic mood and of disturbance in speech and voice were prominent with lithium carbonate. Onset of the therapeutic effect of lithium carbonate was within ten days of medication in 65% of the patients, significantly faster than with chlorpromazine. Side-effects encountered with lithium carbonate therapy at dose levels not higher than 1,800 mg/day were milder and less frequent compared with those seen with chlorpromazine."}
{"id": "PMID:1101837", "title": "[Protective effect of glucose-insulin-potassium solutions in myocardial damage caused by emetine].", "content": "This study was carried out on 170 dogs. Cardiotoxic effects of doses of 15 mg/kg of emetine hydrochloride were studied in 20 dogs. 20 mg/kg of emetine hydrochloride were administered intravenously over 60 min. to the remaining 150 animals. These dogs were distributed in groups of 25. One group received emetine alone. Phleboclyses with saline serum, glucose-insulin-potassium, glocose-insulin, glucose-potassium and glucose serum alone, respectively, were administered to the other 5 groups during the emetine infusion and cardial contraction and several electrograms were recorded. Mean systolic blood pressure was measured continuously in the femoral artery. Observations covered a 4 hour period. During the infusion of emetine alone, ventricular myocardial contraction and systemic arterial pressure decreased abruptly. Intra-atrial, atrio-ventricular and intraventricular conduction disorders, as weel as primary ventricular repolarization changes, were also observed. At the end of this infusion, the Q-T interval increased 25% and the heart rate decreased 26% of the control values. In the group also receiving glucose-insulin-potassium solution, the fall of the ventricular contraction amplitude was significantly less than in animals given emetine alone. Primary ventricular repolarization changes were less evident. The mortality rate reached only 4 per cent. In conclusion, the doses of emetine hydrochloride employed here have depressive cardiocirculatory effects. Glucose-insulin-potassium solution, administered simultaneously with emetine, seems to have a favorable metabolic effect against the emetine cardiotoxicity. This protective action of the G-I-K solution is superior to that obtained with each component, administered separately, and with saline serum.", "contents": "[Protective effect of glucose-insulin-potassium solutions in myocardial damage caused by emetine]. This study was carried out on 170 dogs. Cardiotoxic effects of doses of 15 mg/kg of emetine hydrochloride were studied in 20 dogs. 20 mg/kg of emetine hydrochloride were administered intravenously over 60 min. to the remaining 150 animals. These dogs were distributed in groups of 25. One group received emetine alone. Phleboclyses with saline serum, glucose-insulin-potassium, glocose-insulin, glucose-potassium and glucose serum alone, respectively, were administered to the other 5 groups during the emetine infusion and cardial contraction and several electrograms were recorded. Mean systolic blood pressure was measured continuously in the femoral artery. Observations covered a 4 hour period. During the infusion of emetine alone, ventricular myocardial contraction and systemic arterial pressure decreased abruptly. Intra-atrial, atrio-ventricular and intraventricular conduction disorders, as weel as primary ventricular repolarization changes, were also observed. At the end of this infusion, the Q-T interval increased 25% and the heart rate decreased 26% of the control values. In the group also receiving glucose-insulin-potassium solution, the fall of the ventricular contraction amplitude was significantly less than in animals given emetine alone. Primary ventricular repolarization changes were less evident. The mortality rate reached only 4 per cent. In conclusion, the doses of emetine hydrochloride employed here have depressive cardiocirculatory effects. Glucose-insulin-potassium solution, administered simultaneously with emetine, seems to have a favorable metabolic effect against the emetine cardiotoxicity. This protective action of the G-I-K solution is superior to that obtained with each component, administered separately, and with saline serum."}
{"id": "PMID:1101838", "title": "[Double-blind therapeutic trial of spironolactone in arterial hypertension].", "content": "Spironolactone is a diuretic, selective aldosterone, antagonist with its own antihypertensive action which prevents body loss of potassium. Its clinical use has been documented on the treatment of essential arterial hypertension; however, there does not seem to be enough information to form a solid chemical criterion. This work is a clinical evaluation of the antihypertensive effect of the drug using double-blind technic with 41 patients with essential arterial hypertension, all external patients from the Instituto de Cardiolog\u00eda. They were divided in two groups, selected at random, to be able to observe their tensional range with spironolactone every two weeks, during 16 weeks; and using a placebo for the next 16 weeks. On group following this order; and the other one viceversa. After 32 weeks, the results observed show the effect of placebo, as well as spironolactone on arterial pressure and the statistic comparison, states the real antihypertensive effect of the drug on this group of patients. Every day doses were from 300 to 450 mg., and with it there was a rise in seric potassium of an average of 1 mEq/l. The authors suggest that this drug is useful in cases of essential hypertension although its pathway isn't deeply known. Apparently, the antihypertensive effect of spironolactone is not solely on patients with hypertension due to primary aldosteronism. The intervention on various hipertensinogenous factors in relation to its effect are discussed.", "contents": "[Double-blind therapeutic trial of spironolactone in arterial hypertension]. Spironolactone is a diuretic, selective aldosterone, antagonist with its own antihypertensive action which prevents body loss of potassium. Its clinical use has been documented on the treatment of essential arterial hypertension; however, there does not seem to be enough information to form a solid chemical criterion. This work is a clinical evaluation of the antihypertensive effect of the drug using double-blind technic with 41 patients with essential arterial hypertension, all external patients from the Instituto de Cardiolog\u00eda. They were divided in two groups, selected at random, to be able to observe their tensional range with spironolactone every two weeks, during 16 weeks; and using a placebo for the next 16 weeks. On group following this order; and the other one viceversa. After 32 weeks, the results observed show the effect of placebo, as well as spironolactone on arterial pressure and the statistic comparison, states the real antihypertensive effect of the drug on this group of patients. Every day doses were from 300 to 450 mg., and with it there was a rise in seric potassium of an average of 1 mEq/l. The authors suggest that this drug is useful in cases of essential hypertension although its pathway isn't deeply known. Apparently, the antihypertensive effect of spironolactone is not solely on patients with hypertension due to primary aldosteronism. The intervention on various hipertensinogenous factors in relation to its effect are discussed."}
{"id": "PMID:1101845", "title": "Lithium carbonate response in depression. Prediction by unipolar/bipolar illness, average-evoked response, catechol-O-methyl transferase, and family history.", "content": "The antidepressant efficacy of lithium carbonate was assessed in a double-blind trial in 23 hospitalized depressed patients. Unequivocal response was significantly more frequent in bipolar than in unipolar depressed patients. Lithium carbonate responders had a greater visual average-evoked response amplitude increase in response to increased stimulus intensity, termed \"augmenting\". No correlation could be demonstrated between lithium carbonate efficacy and either erythrocyte catechol-O-methyl transferase activity, age of symptom onset, number of hospitalizations, or family history of affective disorders.", "contents": "Lithium carbonate response in depression. Prediction by unipolar/bipolar illness, average-evoked response, catechol-O-methyl transferase, and family history. The antidepressant efficacy of lithium carbonate was assessed in a double-blind trial in 23 hospitalized depressed patients. Unequivocal response was significantly more frequent in bipolar than in unipolar depressed patients. Lithium carbonate responders had a greater visual average-evoked response amplitude increase in response to increased stimulus intensity, termed \"augmenting\". No correlation could be demonstrated between lithium carbonate efficacy and either erythrocyte catechol-O-methyl transferase activity, age of symptom onset, number of hospitalizations, or family history of affective disorders."}
{"id": "PMID:1101846", "title": "Total sleep deprivation on endogenous depression.", "content": "Ten endogenous depressive patients were deprived of sleep for two whole nights according to the following schedule: sleep/sleep deprivation/sleep/sleep deprivation/sleep. No drugs were administered. Experimental conditions were as neutral as possible. Blind and nonblind ratings were taken. The patients were generally rated as improved after sleep deprivation, but a substantial effect, though temporary with rapid relapse, occurred in only two cases. After subsequent sleep, relapse followed as a rule. The net antidepressive effect of the total procedure was slightly more than nil.", "contents": "Total sleep deprivation on endogenous depression. Ten endogenous depressive patients were deprived of sleep for two whole nights according to the following schedule: sleep/sleep deprivation/sleep/sleep deprivation/sleep. No drugs were administered. Experimental conditions were as neutral as possible. Blind and nonblind ratings were taken. The patients were generally rated as improved after sleep deprivation, but a substantial effect, though temporary with rapid relapse, occurred in only two cases. After subsequent sleep, relapse followed as a rule. The net antidepressive effect of the total procedure was slightly more than nil."}
{"id": "PMID:1101847", "title": "A comparison of antidepressant medications in neurotic and psychotic patients.", "content": "The responses of 225 newly hospitalized depressed women to amitriptyline hydrochloride, imipramine hydrochloride, and thioridazine were compared with particular reference to the psychotic-neurotic distinction. During the first week, more psychotic patients required sedation and more antidepressant medication than did neurotic patients. All treatment groups showed improvement in psychometric criteria after the first week. There was decreasing improvement through the successive weeks, and no statistically significant differences among treatments emerged. Responses of the neurotic group were superior to those of the psychotic group, but there was no psychometric evidence of interaction between diagnostic classification and treatment effect. The results do not support the hypothesis that any one of these treatments is preferable for neurotic patients or for psychotic patients.", "contents": "A comparison of antidepressant medications in neurotic and psychotic patients. The responses of 225 newly hospitalized depressed women to amitriptyline hydrochloride, imipramine hydrochloride, and thioridazine were compared with particular reference to the psychotic-neurotic distinction. During the first week, more psychotic patients required sedation and more antidepressant medication than did neurotic patients. All treatment groups showed improvement in psychometric criteria after the first week. There was decreasing improvement through the successive weeks, and no statistically significant differences among treatments emerged. Responses of the neurotic group were superior to those of the psychotic group, but there was no psychometric evidence of interaction between diagnostic classification and treatment effect. The results do not support the hypothesis that any one of these treatments is preferable for neurotic patients or for psychotic patients."}
{"id": "PMID:1101849", "title": "[Modern nosology and the pathomorphism of kidney diseases].", "content": "In studies of the Bright disease the most promising have proved to be functional-physiological and immunological lines, genetic, morphological and enzymological investigations. Achievements of nephrology in studies of nosological forms have made it possible to describe new disorders, to give different interpretation of the diseases existed earlier, and to fractionate the \"monolith\" nosological forms, which complicates the development of classificational forms of kidney diseases. Singling out of individual disease is impeded, moreover, by the pathomorphosis of kidney diseases, a phenomenon particularly noticeable during the last decade, which not infrequently is difficult to be distinguished from pathology of therapy. The existing classifications of kidney diseases do not meet any longer theoretical and practical needs of current nephrology. In elaborating rational classificational schemes one should be guided not by one but by several principles, including etiological, pathogenetic, clinical, and structural ones, hereditary pathology being considered quite separately.", "contents": "[Modern nosology and the pathomorphism of kidney diseases]. In studies of the Bright disease the most promising have proved to be functional-physiological and immunological lines, genetic, morphological and enzymological investigations. Achievements of nephrology in studies of nosological forms have made it possible to describe new disorders, to give different interpretation of the diseases existed earlier, and to fractionate the \"monolith\" nosological forms, which complicates the development of classificational forms of kidney diseases. Singling out of individual disease is impeded, moreover, by the pathomorphosis of kidney diseases, a phenomenon particularly noticeable during the last decade, which not infrequently is difficult to be distinguished from pathology of therapy. The existing classifications of kidney diseases do not meet any longer theoretical and practical needs of current nephrology. In elaborating rational classificational schemes one should be guided not by one but by several principles, including etiological, pathogenetic, clinical, and structural ones, hereditary pathology being considered quite separately."}
{"id": "PMID:1101850", "title": "[Utilization of luminescent monospecific sera against human immunoglobulins G, A and M for diagnostic purposes].", "content": "A method for obtaining luminescent sera, consisting in isolation of pure antigens G, A, M from human gamma-globulin and blood serum from patients with myeloma disease is suggested. A native antiserum was obtained by immunization of rabbits with water-insoluble polycondensate of antigens \"sewn\" with glutaric aldehyde. Adsorption of antisera as well as specific antigens was carried out with antigen- and antibodyimmunosorbent, the latter being obtained with the help of both glutaric aldehyde and sefarose 4B treated with cyanogen bromide. The sera had a specific titre in the precipitation reaction against their own antigens 1:32 and were highly specific. A globulin fraction was obtained by sedimentation with polyethylene-glycol. Marking of the specific protein with fluoresceine isothiocyanate was carried out using the dialysis method with subsequent purification on sefadex and DEAE-cellulose. The application of the abovementioned sera made it possible to ascertain the character of distribution of deposits of immunoglobulins in glomeruli in systemic lupus erythematodes, glomerulonephritis and in the cells of the synovial fluid sediment in rhematoid arthritis.", "contents": "[Utilization of luminescent monospecific sera against human immunoglobulins G, A and M for diagnostic purposes]. A method for obtaining luminescent sera, consisting in isolation of pure antigens G, A, M from human gamma-globulin and blood serum from patients with myeloma disease is suggested. A native antiserum was obtained by immunization of rabbits with water-insoluble polycondensate of antigens \"sewn\" with glutaric aldehyde. Adsorption of antisera as well as specific antigens was carried out with antigen- and antibodyimmunosorbent, the latter being obtained with the help of both glutaric aldehyde and sefarose 4B treated with cyanogen bromide. The sera had a specific titre in the precipitation reaction against their own antigens 1:32 and were highly specific. A globulin fraction was obtained by sedimentation with polyethylene-glycol. Marking of the specific protein with fluoresceine isothiocyanate was carried out using the dialysis method with subsequent purification on sefadex and DEAE-cellulose. The application of the abovementioned sera made it possible to ascertain the character of distribution of deposits of immunoglobulins in glomeruli in systemic lupus erythematodes, glomerulonephritis and in the cells of the synovial fluid sediment in rhematoid arthritis."}
{"id": "PMID:1101851", "title": "[Morphometric study of the thyroid gland].", "content": "The article surveys the most important morphometric methods used for investigating the thyroid gland. Applicability of various histophysiological criteria for evaluation of the functional state of the organ and investigation of changes in its individual structural components in physiological and pathological processes is discussed. The connection between various quantitative indices and the most rational morphometric techniques based on the methods of planimetry and stereology are considered.", "contents": "[Morphometric study of the thyroid gland]. The article surveys the most important morphometric methods used for investigating the thyroid gland. Applicability of various histophysiological criteria for evaluation of the functional state of the organ and investigation of changes in its individual structural components in physiological and pathological processes is discussed. The connection between various quantitative indices and the most rational morphometric techniques based on the methods of planimetry and stereology are considered."}
{"id": "PMID:1101854", "title": "[Characteristics of the pathomorphological manifestation of salmonella infection in guinea pigs and hamsters].", "content": "Observations on the manifestations of salmonellosis infection in various species of laboratory animals made it possible to establish general regularities inherent to this infectious disease, to reveal features depending on metabolic, morphological and microbiological characteristics of a given species of animals. These comparative data are of importance for understanding the factors determining resistance or sensitivity of the macroorganism to infection. Pathomorphological and microbiological investigations of experimental salmonellosis infection in hamsters and guinea-pigs showed that changes characteristic of this infection were manifested considerably weaker in guinea pigs than in hamsters. Of special interest were lesions of the large intestine mucosa wall in guinea pigs and those of the lungs in hamsters. These data may be a subject of a special study. Development and course of the experimental infection were not accompanied by the development of disbacteriosis. Pathogenic bacteria were eliminated on the 2nd--4th day in guinea pigs and on the 5--7th day in hamsters.", "contents": "[Characteristics of the pathomorphological manifestation of salmonella infection in guinea pigs and hamsters]. Observations on the manifestations of salmonellosis infection in various species of laboratory animals made it possible to establish general regularities inherent to this infectious disease, to reveal features depending on metabolic, morphological and microbiological characteristics of a given species of animals. These comparative data are of importance for understanding the factors determining resistance or sensitivity of the macroorganism to infection. Pathomorphological and microbiological investigations of experimental salmonellosis infection in hamsters and guinea-pigs showed that changes characteristic of this infection were manifested considerably weaker in guinea pigs than in hamsters. Of special interest were lesions of the large intestine mucosa wall in guinea pigs and those of the lungs in hamsters. These data may be a subject of a special study. Development and course of the experimental infection were not accompanied by the development of disbacteriosis. Pathogenic bacteria were eliminated on the 2nd--4th day in guinea pigs and on the 5--7th day in hamsters."}
{"id": "PMID:1101856", "title": "[Listeriosis in agricultural animals (problems of the epizootiology, pathological anatomy and pathogenesis)].", "content": "Data are presented on the main problems of epizootology, pathological anatomy and pathogenesis of listeriosis of agricultural animals. It was shown that under natural conditions all species of agricultural animals are susceptible to the condition but ship are affected most often. Various clinico-anatomic forms of the disease are considered: nervous, septicemic, metrogenic, mixed, subclinical and latent. Domestic animals were observed to suffer predominantly from the nervous form of the disease. Basing on literature reports and his own findings, the author elucidates with greater detail problems of pathomorphology and pathogenesis of the nervous form of listeriosis and shows the importance of the neurogenic pathway in the development of listerious encephalomyelitis.", "contents": "[Listeriosis in agricultural animals (problems of the epizootiology, pathological anatomy and pathogenesis)]. Data are presented on the main problems of epizootology, pathological anatomy and pathogenesis of listeriosis of agricultural animals. It was shown that under natural conditions all species of agricultural animals are susceptible to the condition but ship are affected most often. Various clinico-anatomic forms of the disease are considered: nervous, septicemic, metrogenic, mixed, subclinical and latent. Domestic animals were observed to suffer predominantly from the nervous form of the disease. Basing on literature reports and his own findings, the author elucidates with greater detail problems of pathomorphology and pathogenesis of the nervous form of listeriosis and shows the importance of the neurogenic pathway in the development of listerious encephalomyelitis."}
{"id": "PMID:1101861", "title": "Scleral perforation. A complication of the soft contact lens.", "content": "A large area of conjunctiva and a smaller area of full thickness sclera eroded immediately adjacent to the edge of a soft contact lens. The soft lens was worn to protect the epithelium of a corneal graft in an alkali-burned eye. The conjunctiva-sclera necrosis appears to be a complication of a distorted and tightly fitting lens.", "contents": "Scleral perforation. A complication of the soft contact lens. A large area of conjunctiva and a smaller area of full thickness sclera eroded immediately adjacent to the edge of a soft contact lens. The soft lens was worn to protect the epithelium of a corneal graft in an alkali-burned eye. The conjunctiva-sclera necrosis appears to be a complication of a distorted and tightly fitting lens."}
{"id": "PMID:1101862", "title": "Fat embolism: physiopathology, diagnosis with management.", "content": "This paper describes the components of the post-traumatic syndrome. The pathological state of fat macroglobulinemia by occlusion of arteriolar terminal branches of vital organs causes the fat embolic syndrome. The main physiopathological problem is pulmonary insufficiency in an acute or subacute form. The cerebral embolus is second. The symptomatology varies and is not characteristic. According to their importance they were described as minor or major in the diagnosis of fat emboli. Only recently, treatment for the fat emboli syndrome has been initiated, consisting mainly in supportive measures, respiratory care and anti-inflammatory drugs, mainly cortisone.", "contents": "Fat embolism: physiopathology, diagnosis with management. This paper describes the components of the post-traumatic syndrome. The pathological state of fat macroglobulinemia by occlusion of arteriolar terminal branches of vital organs causes the fat embolic syndrome. The main physiopathological problem is pulmonary insufficiency in an acute or subacute form. The cerebral embolus is second. The symptomatology varies and is not characteristic. According to their importance they were described as minor or major in the diagnosis of fat emboli. Only recently, treatment for the fat emboli syndrome has been initiated, consisting mainly in supportive measures, respiratory care and anti-inflammatory drugs, mainly cortisone."}
{"id": "PMID:1101863", "title": "[Longitudinal growth of the tibia using the model of a congenital pseudarthrosis of the tibia (author's transl)].", "content": "Authors could not confirm a growth disturbance--suggested by other workers--at the distal end of the tibia in a modell experiment of congenital pseudarthrosis of the leg.", "contents": "[Longitudinal growth of the tibia using the model of a congenital pseudarthrosis of the tibia (author's transl)]. Authors could not confirm a growth disturbance--suggested by other workers--at the distal end of the tibia in a modell experiment of congenital pseudarthrosis of the leg."}
{"id": "PMID:1101864", "title": "[Elongation of the anterior cruciate ligament--reefing or displacing (author's transl)].", "content": "Among old injuries involving the cruciate ligaments elongation of the anterior cruciate ligament is found in 20%. Follow-up examinations revealed that the purse-string suture technique does not guarantee permanent tightness of the anterior cruciate ligament. In consideration of the unsatisfactory results obtained by the purse-string suture technique it is suggested to effect tightness of the anterior cruciate ligament by the transfer of its distal insertion. The technique is described.", "contents": "[Elongation of the anterior cruciate ligament--reefing or displacing (author's transl)]. Among old injuries involving the cruciate ligaments elongation of the anterior cruciate ligament is found in 20%. Follow-up examinations revealed that the purse-string suture technique does not guarantee permanent tightness of the anterior cruciate ligament. In consideration of the unsatisfactory results obtained by the purse-string suture technique it is suggested to effect tightness of the anterior cruciate ligament by the transfer of its distal insertion. The technique is described."}
{"id": "PMID:1101865", "title": "A radiographic evaluation of microporosity in a nickel base casting allow.", "content": "Three series of tensile test pieces were produced using a nickel base partial denture casting alloy. For the first series induction heating was employed, for the second a resistance crucible, and for the third an oxy-acetylene torch. All specimens were radiographed and a classification developed to indicate the radiographic soundness of each specimen. Radiographic soundness was subsequently related to the results obtained from mechanical testing.", "contents": "A radiographic evaluation of microporosity in a nickel base casting allow. Three series of tensile test pieces were produced using a nickel base partial denture casting alloy. For the first series induction heating was employed, for the second a resistance crucible, and for the third an oxy-acetylene torch. All specimens were radiographed and a classification developed to indicate the radiographic soundness of each specimen. Radiographic soundness was subsequently related to the results obtained from mechanical testing."}
{"id": "PMID:1101866", "title": "The value of associating pentazocine with drugs used in accepted intravenous techniques.", "content": "Pentazocine, a systemic analgesic, can be utilized in local analgesic and intravenous techniques to provide better operating conditions in difficult cases, a reduction in the dosage of other agents employed, and a smoother recovery period.", "contents": "The value of associating pentazocine with drugs used in accepted intravenous techniques. Pentazocine, a systemic analgesic, can be utilized in local analgesic and intravenous techniques to provide better operating conditions in difficult cases, a reduction in the dosage of other agents employed, and a smoother recovery period."}
{"id": "PMID:1101868", "title": "Hepatitis B antigen, auto-antibodies and liver disease in a haemodialysis and transplantation unit.", "content": "This study concerns hepatitis B antigen and antibody, (HBAg and HBAb) auto-antibodies and liver disease in a renal haemodialysis and transplantation unit between February, 1970 and December, 1972. Fourteen per cent of patients on maintenance haemodialysis, 25% of renal transplant recipients, and 5% of the staff were HBAg positive during this period. Three family members of antigen positive patients developed hepatitis; all were either antigen or antibody positive. Both immuno-electro-osmo-phoresis and radio-immuno-assay were used to detect HBAg. No additional carriers were identified among the dialysis or transplant patients by applying the more sensitive technique, although two persons with hepatitis were positive only by RIA. None of the staff members who developed antigen positive hepatitis died, and no one became a carrier. Dialysis patients who had icteric hepatis also cleared the antigen from the serum. Clinical hepatitis did not occur in immunosuppressed graft recipients who were carriers of the ay subtype of the antigen, nor was there histological evidence of liver disease in 18 of 19 of such carriers studied. Moreover, there was no association between long term carriage of the antigen and the presence of the auto-antibodies characteristic of chronic active hepatitis. Rapid reduction of immunosuppression was followed by acute hepatitis in one carrier, while another, whose antigen was of the ad subtype, developed micro-nodular cirrhosis.", "contents": "Hepatitis B antigen, auto-antibodies and liver disease in a haemodialysis and transplantation unit. This study concerns hepatitis B antigen and antibody, (HBAg and HBAb) auto-antibodies and liver disease in a renal haemodialysis and transplantation unit between February, 1970 and December, 1972. Fourteen per cent of patients on maintenance haemodialysis, 25% of renal transplant recipients, and 5% of the staff were HBAg positive during this period. Three family members of antigen positive patients developed hepatitis; all were either antigen or antibody positive. Both immuno-electro-osmo-phoresis and radio-immuno-assay were used to detect HBAg. No additional carriers were identified among the dialysis or transplant patients by applying the more sensitive technique, although two persons with hepatitis were positive only by RIA. None of the staff members who developed antigen positive hepatitis died, and no one became a carrier. Dialysis patients who had icteric hepatis also cleared the antigen from the serum. Clinical hepatitis did not occur in immunosuppressed graft recipients who were carriers of the ay subtype of the antigen, nor was there histological evidence of liver disease in 18 of 19 of such carriers studied. Moreover, there was no association between long term carriage of the antigen and the presence of the auto-antibodies characteristic of chronic active hepatitis. Rapid reduction of immunosuppression was followed by acute hepatitis in one carrier, while another, whose antigen was of the ad subtype, developed micro-nodular cirrhosis."}
{"id": "PMID:1101870", "title": "The surgical management of subphrenic abscess: a historical study.", "content": "This paper is a study of the evolution of the management of subphrenic abscess from the earliest reports to the present day. Its purpose is to compare and contrast the attitudes and practices established during the earlier years with those of the present, in particular in relation to changes consequent on the introduction of antibiotics.", "contents": "The surgical management of subphrenic abscess: a historical study. This paper is a study of the evolution of the management of subphrenic abscess from the earliest reports to the present day. Its purpose is to compare and contrast the attitudes and practices established during the earlier years with those of the present, in particular in relation to changes consequent on the introduction of antibiotics."}
{"id": "PMID:1101871", "title": "Experimental gut anastomoses and their revascularization.", "content": "A study was made of the healing and revascularization of intestinal anastomoses in the small and large intestines of rabbits. Single-layer and double-layer suture, techniques were used and compared, and revascularization of the anastomoses was particularly examined after devascularization of their distal segments. The anastomotic segments were removed at intervals after construction varying from one to six weeks and studied histologically after haemotoxylin and eosin staining, by xylol-clear thick sections and by angiography, a gelatin Micropaque emulsion being used to fill the vascular network. Forty-four rabbit intestinal anastomoses were studied. Inverted intestinal anastomoses took about two weeks to develop continuous mucosa on the luminal side, and were completely healed after six weeks. Revascularization began after eight days with both single-layer and double-layer anastomoses, proceeding from the submucosal and the surrounding muscular layers. The vascular pattern mirrored the healing stage of the anastomosis. Irregular fine vessels crossing the anastomoses were seen in specimens examined at the second week. After three or four weeks, the vascular patterns of the anastomoses showed an irregularity of calibre and direction because of the presence of residual scar tissue, and the mucosal surfaces were covered with regenerating epithelium. Healing and revascularization were complete after six weeks.", "contents": "Experimental gut anastomoses and their revascularization. A study was made of the healing and revascularization of intestinal anastomoses in the small and large intestines of rabbits. Single-layer and double-layer suture, techniques were used and compared, and revascularization of the anastomoses was particularly examined after devascularization of their distal segments. The anastomotic segments were removed at intervals after construction varying from one to six weeks and studied histologically after haemotoxylin and eosin staining, by xylol-clear thick sections and by angiography, a gelatin Micropaque emulsion being used to fill the vascular network. Forty-four rabbit intestinal anastomoses were studied. Inverted intestinal anastomoses took about two weeks to develop continuous mucosa on the luminal side, and were completely healed after six weeks. Revascularization began after eight days with both single-layer and double-layer anastomoses, proceeding from the submucosal and the surrounding muscular layers. The vascular pattern mirrored the healing stage of the anastomosis. Irregular fine vessels crossing the anastomoses were seen in specimens examined at the second week. After three or four weeks, the vascular patterns of the anastomoses showed an irregularity of calibre and direction because of the presence of residual scar tissue, and the mucosal surfaces were covered with regenerating epithelium. Healing and revascularization were complete after six weeks."}
{"id": "PMID:1101884", "title": "Circadian changes in the temperature of human beings.", "content": "Temperature in man measured or recorded by different means, is not constant but varies in a predictable and rhythmic fashion. Circadian (about 24-hour) rhythms have been demonstrated and studied in healthy men and women as well as in patients under a wide variety of experimental conditions and diseases. With the help of special computer programs (Halberg's cosinor) inferential statistical analyses can be performed. There validate and characterize a biological rhythm (i.e., core temperature) by several parameters: the period (tau) the acrophase (phi) (timing of the peak), the amplitude (A) and the rhythm-adjusted mean (M). Each one of these parameters is given with its confidence limits when the studied rhythm is detectable (p less than 0.05). The human temperature (oral, rectal and skin) circadian rhythm has been validated and quantified in healthy newborns, in healthy adults on various diets (including near-fasting conditions: 220 cal/24h, casein) and various type of activities. Food intake does not appear to influence the temperature circadian rhythm. The rhythm persists with a change of period and/or acrophase during isolation underground, without time dues or clues. Its acrophase can be shifted by manipulating synchronizing factors (i.e., shift-working, transmeridian flight). Alteration of circadian temperature rhythm may result from the timed administration of certain drugs (i.e. reserpine) and from certain chronic diseases (without overt fever). A first attempt to use both thermography and chronobiological method has been made independantly by Gautherie et al [40] and Smolensky [41] in the prediction of therapeutic value of a given modality during the course of breast cancer treatment.", "contents": "Circadian changes in the temperature of human beings. Temperature in man measured or recorded by different means, is not constant but varies in a predictable and rhythmic fashion. Circadian (about 24-hour) rhythms have been demonstrated and studied in healthy men and women as well as in patients under a wide variety of experimental conditions and diseases. With the help of special computer programs (Halberg's cosinor) inferential statistical analyses can be performed. There validate and characterize a biological rhythm (i.e., core temperature) by several parameters: the period (tau) the acrophase (phi) (timing of the peak), the amplitude (A) and the rhythm-adjusted mean (M). Each one of these parameters is given with its confidence limits when the studied rhythm is detectable (p less than 0.05). The human temperature (oral, rectal and skin) circadian rhythm has been validated and quantified in healthy newborns, in healthy adults on various diets (including near-fasting conditions: 220 cal/24h, casein) and various type of activities. Food intake does not appear to influence the temperature circadian rhythm. The rhythm persists with a change of period and/or acrophase during isolation underground, without time dues or clues. Its acrophase can be shifted by manipulating synchronizing factors (i.e., shift-working, transmeridian flight). Alteration of circadian temperature rhythm may result from the timed administration of certain drugs (i.e. reserpine) and from certain chronic diseases (without overt fever). A first attempt to use both thermography and chronobiological method has been made independantly by Gautherie et al [40] and Smolensky [41] in the prediction of therapeutic value of a given modality during the course of breast cancer treatment."}
{"id": "PMID:1101885", "title": "Comparative studies on subacute sclerosing panencephalitis virus and a usual measles virus with special reference to their capacities to produce viral hemagglutinin in Vero cells.", "content": "Various comparative studies were made on the capacities of a cell-associated subacute sclerosing panencephalitis agent (SSPE agent, Niigata/1 strain) and a usual measles virus (Toyoshima strain) to produce measles virus hemagglutinin (MV-HAnim) in Vero cells. The results obtained by various methods, such as the hemagglutination test (HA), hemadsorption test (HAd), antibody blocking test, antibody inducing test and fluorescent antibody technique (FAT) using monospecific antibody to MV-HAnin, consistently suggested that the SSPE agent was an incomplete measles-like virus having little if any MV-HAnin, but sharing immunologically closely related viral components other than MV-HAnin with those of the usual measles virus. The significance of these findings is discussed with respects to the latency of the SSPE agent.", "contents": "Comparative studies on subacute sclerosing panencephalitis virus and a usual measles virus with special reference to their capacities to produce viral hemagglutinin in Vero cells. Various comparative studies were made on the capacities of a cell-associated subacute sclerosing panencephalitis agent (SSPE agent, Niigata/1 strain) and a usual measles virus (Toyoshima strain) to produce measles virus hemagglutinin (MV-HAnim) in Vero cells. The results obtained by various methods, such as the hemagglutination test (HA), hemadsorption test (HAd), antibody blocking test, antibody inducing test and fluorescent antibody technique (FAT) using monospecific antibody to MV-HAnin, consistently suggested that the SSPE agent was an incomplete measles-like virus having little if any MV-HAnin, but sharing immunologically closely related viral components other than MV-HAnin with those of the usual measles virus. The significance of these findings is discussed with respects to the latency of the SSPE agent."}
{"id": "PMID:1101897", "title": "[The influence on pedicle flap blood supply and necrosis rate of plastic displacement with pentoxifylline and dextran 40].", "content": "Using a standardized procedure we have experimented on rats in order to examine the rate of necrosis in pedicle flaps in conjunction with 3,7-dimethyl-1-(5-oxo-hexyl)-xanthine (pentoxifyline, Trental) and dextran 40. Compared with the control group and that treated with dextran 40 the group treated with Trental showed significant decrease in necroses. The influence of drugs on blood supply demonstrated in this srudy as being significant is discussed.", "contents": "[The influence on pedicle flap blood supply and necrosis rate of plastic displacement with pentoxifylline and dextran 40]. Using a standardized procedure we have experimented on rats in order to examine the rate of necrosis in pedicle flaps in conjunction with 3,7-dimethyl-1-(5-oxo-hexyl)-xanthine (pentoxifyline, Trental) and dextran 40. Compared with the control group and that treated with dextran 40 the group treated with Trental showed significant decrease in necroses. The influence of drugs on blood supply demonstrated in this srudy as being significant is discussed."}
{"id": "PMID:1101898", "title": "[The effect of oxyfedrine on cardiac performance in coronary patients. Tests using electrocardiographic and metabolic parameters in atrial stimulation].", "content": "In 17 patients with coronary heart disease the effects of the beta-active agent L-3-(beta-hydroxy-alpha-methyl-phenethyl-amino)-3'-methoxy-propiophenone (oxyfedrine, Ildamen) were proved at rest and during atrial pacing by measurements of coronary venous O2-saturation, myocardial lactate extraction, angina threshold and ST-segment depression. In 88% oxyfedrine had antianginal effectivity with rise in angina threshold (+11%), reduction of ST-segment depression (--48%) and reduction of lactate production(--63%). The rise in coronary venous O2-saturation (\"35%) and the electrocardiographic and metabolic reduction of hypoxic reaction indicate the improvement of nutritional coronary flow. Therefore improvement of myocardial O2-balance is derived from the reduced energy requirement by decrease of heart size and ventricular wall tension and the rise of myocardial O2-supply by coronary dilation in spite of the increased energy requirement by ascending contractility. Conclusively the application of oxyfedrine is preferred in coronary heart disease with simultaneous congestive heart failure.", "contents": "[The effect of oxyfedrine on cardiac performance in coronary patients. Tests using electrocardiographic and metabolic parameters in atrial stimulation]. In 17 patients with coronary heart disease the effects of the beta-active agent L-3-(beta-hydroxy-alpha-methyl-phenethyl-amino)-3'-methoxy-propiophenone (oxyfedrine, Ildamen) were proved at rest and during atrial pacing by measurements of coronary venous O2-saturation, myocardial lactate extraction, angina threshold and ST-segment depression. In 88% oxyfedrine had antianginal effectivity with rise in angina threshold (+11%), reduction of ST-segment depression (--48%) and reduction of lactate production(--63%). The rise in coronary venous O2-saturation (\"35%) and the electrocardiographic and metabolic reduction of hypoxic reaction indicate the improvement of nutritional coronary flow. Therefore improvement of myocardial O2-balance is derived from the reduced energy requirement by decrease of heart size and ventricular wall tension and the rise of myocardial O2-supply by coronary dilation in spite of the increased energy requirement by ascending contractility. Conclusively the application of oxyfedrine is preferred in coronary heart disease with simultaneous congestive heart failure."}
{"id": "PMID:1101900", "title": "Toxicological and clinical aspects of cyanide metabolism.", "content": "This contribution deals with the occurrence of cyanide and its biological pathways in the body. Especially possibilities of detoxification are pointed out. Intoxications are caused by acute and chronical cyanide uptake. Tobacco amblyopia, retrobulbar neuritis in pernicious anaemia, Leber's optic atrophy, Nigerian nutritional neuropathy, and sterility in female heavy smokers are attributed to cyanide intoxication. Various methods for treating acute and chronic cyanide intoxication are discussed.", "contents": "Toxicological and clinical aspects of cyanide metabolism. This contribution deals with the occurrence of cyanide and its biological pathways in the body. Especially possibilities of detoxification are pointed out. Intoxications are caused by acute and chronical cyanide uptake. Tobacco amblyopia, retrobulbar neuritis in pernicious anaemia, Leber's optic atrophy, Nigerian nutritional neuropathy, and sterility in female heavy smokers are attributed to cyanide intoxication. Various methods for treating acute and chronic cyanide intoxication are discussed."}
{"id": "PMID:1101901", "title": "A new isoniazid preparation designed for moderately fast and \"fast\" metabolizers of the drug.", "content": "The bioavailability of a new isoniazid preparation consisting of 37% ordinary isoniazid (INH) and 63% matrix component was investigated in 11 slow, 8 moderately fast, and 9 \"fast\" acetylators of the drug. Initially, a 15 mg/kg dose of normal INH was administered to all the participants. In addition, the two groups of fast metabolizers received 30 and 45 mg INH-matrix/kg, respectively, with a one-week interval between the test doses. These high dosages of the INH-matrix could be given without encountering toxic reactions because of the delayed absorption of the matrix formulation. In moderately fast acetylators, with a triple dose of matrix isoniazid, it was possible to mimic the blood levels produced by 15 mg ordinary INH/kg in slow inactivators. However, in \"fast\" acetylators the blood concentrations achieved with the 45 mg/kg dose of INH-matrix were somewhat lower. This study also showed that a large input rate is essential to procure the required, high blood levels in fast metabolizers. The therapeutic implications of the results of this bioavailability trial are discussed.", "contents": "A new isoniazid preparation designed for moderately fast and \"fast\" metabolizers of the drug. The bioavailability of a new isoniazid preparation consisting of 37% ordinary isoniazid (INH) and 63% matrix component was investigated in 11 slow, 8 moderately fast, and 9 \"fast\" acetylators of the drug. Initially, a 15 mg/kg dose of normal INH was administered to all the participants. In addition, the two groups of fast metabolizers received 30 and 45 mg INH-matrix/kg, respectively, with a one-week interval between the test doses. These high dosages of the INH-matrix could be given without encountering toxic reactions because of the delayed absorption of the matrix formulation. In moderately fast acetylators, with a triple dose of matrix isoniazid, it was possible to mimic the blood levels produced by 15 mg ordinary INH/kg in slow inactivators. However, in \"fast\" acetylators the blood concentrations achieved with the 45 mg/kg dose of INH-matrix were somewhat lower. This study also showed that a large input rate is essential to procure the required, high blood levels in fast metabolizers. The therapeutic implications of the results of this bioavailability trial are discussed."}
{"id": "PMID:1101902", "title": "An X-ray study on the acute action of diphenoxylate on the intestinal motility in volunteers.", "content": "The intestinal inhibitory activity of 1-(3-cyano-3,3-diphenylpropyl)-4-phenyl-4-piperidine-carboxylic acid ethyl ester (diphenoxylate) after a single oral dose of 5 mg was studied in 10 volunteers with X-ray diagnostic methods. The diphenoxylate treatment lengthened the transit time of the barium sulphate to caecum from 3.2 to 4.3 h. The X-ray cinematography showed the slower duodenal emptying in 5/10 of the subjects, but only in 1 of these the velocity of the peristaltic wave was clearly decreased. The results demonstrate directly the acute inhibitory action of diphenoxylate on intestinal motility with the dose used generally 3--4 times daily for the symptomatic treatment of diarrhoea.", "contents": "An X-ray study on the acute action of diphenoxylate on the intestinal motility in volunteers. The intestinal inhibitory activity of 1-(3-cyano-3,3-diphenylpropyl)-4-phenyl-4-piperidine-carboxylic acid ethyl ester (diphenoxylate) after a single oral dose of 5 mg was studied in 10 volunteers with X-ray diagnostic methods. The diphenoxylate treatment lengthened the transit time of the barium sulphate to caecum from 3.2 to 4.3 h. The X-ray cinematography showed the slower duodenal emptying in 5/10 of the subjects, but only in 1 of these the velocity of the peristaltic wave was clearly decreased. The results demonstrate directly the acute inhibitory action of diphenoxylate on intestinal motility with the dose used generally 3--4 times daily for the symptomatic treatment of diarrhoea."}
{"id": "PMID:1101903", "title": "A double-blind clinical study on long-term use of oxyphenbutazone and tolfenamic acid in connection with telecobalt therapy.", "content": "Thirty-one patients who suffered from various cancers in the pelvic region were treated in a double-blind clinical long-term study either with placebo, N-(2-methyl-3-chlorophenyl)-anthranilic acid (tolfenamic acid, Clotam) or oxyphenbutazone in order to diminish the local reactions after telecobalt therapy. The results were difficult to judge and no clear differences were seen between the groups. The side-effects were slight and unspecific, except gastrointestinal distresses, which occurred with both tolfenamic acid and oxyphenbutazone, but were more severe with the latter. No significant changes were seen in the blood counts, liver or kidney function tests with tolfenamic acid. In the oxyphenbutazone group S-ALAT, S-AFOS and direct bilirubin were slightly elevated. Blood in feces was more frequent in the drug-treated than in the placebo group. In the light of the present and earlier studies tolfenamic acid appears to cause less side-effects than oxyphenbutazone in prolonged treatment.", "contents": "A double-blind clinical study on long-term use of oxyphenbutazone and tolfenamic acid in connection with telecobalt therapy. Thirty-one patients who suffered from various cancers in the pelvic region were treated in a double-blind clinical long-term study either with placebo, N-(2-methyl-3-chlorophenyl)-anthranilic acid (tolfenamic acid, Clotam) or oxyphenbutazone in order to diminish the local reactions after telecobalt therapy. The results were difficult to judge and no clear differences were seen between the groups. The side-effects were slight and unspecific, except gastrointestinal distresses, which occurred with both tolfenamic acid and oxyphenbutazone, but were more severe with the latter. No significant changes were seen in the blood counts, liver or kidney function tests with tolfenamic acid. In the oxyphenbutazone group S-ALAT, S-AFOS and direct bilirubin were slightly elevated. Blood in feces was more frequent in the drug-treated than in the placebo group. In the light of the present and earlier studies tolfenamic acid appears to cause less side-effects than oxyphenbutazone in prolonged treatment."}
{"id": "PMID:1101904", "title": "Effect of two weeks' treatment with chlordiazepoxide or flupenthixole, alone or in combination with alcohol, on psychomotor skills related to driving.", "content": "The effects of two weeks' treatment with chlordiazepoxide 10 mg t.i.d. or flupenthixole 0.5 mg t.i.d. on human psychomotor performance related to driving was examined on 20 healthy male students aged 20 to 23 years. The tests used were a choice reaction, two co-ordination and an attention test having correlation with traffic behaviour. Neither chlordiazepoxide nor flupenthixole impaired psychomotor performance on the 7th or 14th days of the experiment. The combination of either drug with 0.5 g/kg of alcohol impaired co-ordination and attention to an extent which can be considered dangerous for traffic and occupational life. Their interaction with alcohol was not so strong as that between diazepam and alcohol. The combination of chlordiazepoxide with alcohol tended to increase the anxiety of the normal subjects.", "contents": "Effect of two weeks' treatment with chlordiazepoxide or flupenthixole, alone or in combination with alcohol, on psychomotor skills related to driving. The effects of two weeks' treatment with chlordiazepoxide 10 mg t.i.d. or flupenthixole 0.5 mg t.i.d. on human psychomotor performance related to driving was examined on 20 healthy male students aged 20 to 23 years. The tests used were a choice reaction, two co-ordination and an attention test having correlation with traffic behaviour. Neither chlordiazepoxide nor flupenthixole impaired psychomotor performance on the 7th or 14th days of the experiment. The combination of either drug with 0.5 g/kg of alcohol impaired co-ordination and attention to an extent which can be considered dangerous for traffic and occupational life. Their interaction with alcohol was not so strong as that between diazepam and alcohol. The combination of chlordiazepoxide with alcohol tended to increase the anxiety of the normal subjects."}
{"id": "PMID:1101905", "title": "[Biochemical action mechanisms and pharmacokinetics of psychoactive drugs].", "content": "A short review is given on the effects of antidepressant drugs (tricyclic thymoleptics and MAO inhibitors), neuroleptics and lithium salts on catecholamine and serotonin metabolisms in brain. The second part deals with some pharmacokinetic investigations with these psychotropic drugs. In connection with tricyclic thymoleptics some factors influencing steady-state plasma concentration and their relation to the therapeutic effect are discussed. Furthermore, recent investigations into the pharmacokinetics of lithium salts are dealt with.", "contents": "[Biochemical action mechanisms and pharmacokinetics of psychoactive drugs]. A short review is given on the effects of antidepressant drugs (tricyclic thymoleptics and MAO inhibitors), neuroleptics and lithium salts on catecholamine and serotonin metabolisms in brain. The second part deals with some pharmacokinetic investigations with these psychotropic drugs. In connection with tricyclic thymoleptics some factors influencing steady-state plasma concentration and their relation to the therapeutic effect are discussed. Furthermore, recent investigations into the pharmacokinetics of lithium salts are dealt with."}
{"id": "PMID:1101906", "title": "[Data acquisition in clinical trials].", "content": "Methods of data acquisition in clinical trials are reviewed and their advantages and disadvantages are discussed. To avoid the effort of multiple data acquisition and to eliminate possible transcription errors the data of the patients should be collected and stored in a data bank only once also in case of a drug study. From the data bank the results can be used for the different clinical purposes as well as for the statistical evaluation when testing drugs.", "contents": "[Data acquisition in clinical trials]. Methods of data acquisition in clinical trials are reviewed and their advantages and disadvantages are discussed. To avoid the effort of multiple data acquisition and to eliminate possible transcription errors the data of the patients should be collected and stored in a data bank only once also in case of a drug study. From the data bank the results can be used for the different clinical purposes as well as for the statistical evaluation when testing drugs."}
{"id": "PMID:1101907", "title": "[Comparison of the effects of niflumic acid and aloxiprin in patients with progressive chronic rheumatoid arthritis in a double blind trial].", "content": "The effects of niflumic acid (trifluoro-methyl-3-phenylamino-2-nicotinic acid) and of aloxiprin (aluminium acetyl-salicylate) in patients with rheumatoid arthritis were compared in a double-blind cross-over trial. Check-up examinations prior to the institution and after the termination of the therapy, as well as after individual treatment periods of two weeks with alternating sequential administration of the two drugs, were carried out with a standardized method. The assessment included subjective (pain intensity) and objective criteria (Lansbury's index), as well as laboratory results (erythrocyte sedimentation rate, uricaemia). With the exception of the articular index disclosing a superior effect of niflumic acid, the other criteria showed no statistically significant differences between the effects of the two drugs.", "contents": "[Comparison of the effects of niflumic acid and aloxiprin in patients with progressive chronic rheumatoid arthritis in a double blind trial]. The effects of niflumic acid (trifluoro-methyl-3-phenylamino-2-nicotinic acid) and of aloxiprin (aluminium acetyl-salicylate) in patients with rheumatoid arthritis were compared in a double-blind cross-over trial. Check-up examinations prior to the institution and after the termination of the therapy, as well as after individual treatment periods of two weeks with alternating sequential administration of the two drugs, were carried out with a standardized method. The assessment included subjective (pain intensity) and objective criteria (Lansbury's index), as well as laboratory results (erythrocyte sedimentation rate, uricaemia). With the exception of the articular index disclosing a superior effect of niflumic acid, the other criteria showed no statistically significant differences between the effects of the two drugs."}
{"id": "PMID:1101908", "title": "[Studies on the influence of pentosanepolysulfate and a combination of pentosanepolysulfate, metamizol and paracetamol on inflammatory liability (author's transl)].", "content": "The influence of pentosanepolysulfate (SP 54) and a combination of pentosanepolysulfate, metamizol and paracetamol (Probaphen) on the course of an experimentally induced erythema caused by intracutaneous injection of a lipopolysaccharide from Pseudomonas has been studied in man. The intramuscular injection of Probaphen as well as SP 54, separately given, showed a significant antiinflammatory effect. A similar effect was also achieved after administration of Probaphen suppositories. Probaphen shortened the inflammatory reaction more effectively than did SP 54 alone. The oral application of Probaphen, however, did not influence the course of inflammation.", "contents": "[Studies on the influence of pentosanepolysulfate and a combination of pentosanepolysulfate, metamizol and paracetamol on inflammatory liability (author's transl)]. The influence of pentosanepolysulfate (SP 54) and a combination of pentosanepolysulfate, metamizol and paracetamol (Probaphen) on the course of an experimentally induced erythema caused by intracutaneous injection of a lipopolysaccharide from Pseudomonas has been studied in man. The intramuscular injection of Probaphen as well as SP 54, separately given, showed a significant antiinflammatory effect. A similar effect was also achieved after administration of Probaphen suppositories. Probaphen shortened the inflammatory reaction more effectively than did SP 54 alone. The oral application of Probaphen, however, did not influence the course of inflammation."}
{"id": "PMID:1101909", "title": "[Comparative studies on wholebody retention of iron(II)-sulfate and a combination of iron(II)-sulfate and DL-serine].", "content": "The wholebody retention of an 59iron-sulphate-DL-serin combination (group B) was compared to that of 59iron-sulphate in healthy female volunteers. Mean 59Fe-retention of group B(n = 9) was found to be 40% higher than that of group A(n = 10); however, this difference was mathematically statistically not significant--especially because of the high standard deviation of group B.", "contents": "[Comparative studies on wholebody retention of iron(II)-sulfate and a combination of iron(II)-sulfate and DL-serine]. The wholebody retention of an 59iron-sulphate-DL-serin combination (group B) was compared to that of 59iron-sulphate in healthy female volunteers. Mean 59Fe-retention of group B(n = 9) was found to be 40% higher than that of group A(n = 10); however, this difference was mathematically statistically not significant--especially because of the high standard deviation of group B."}
{"id": "PMID:1101910", "title": "Are hormones psychoactive? Evoked potential investigations in man.", "content": "The somatosensory evoked potential (SEP) of physically and mentally healthy male subjects was recorded before as well as 4 hours after administration of one single dose of placebo, cyproterone acetate (an antiandrogen), and mesterolone (an androgen). Quantitative evaluation of drug-induced changes in SEP latencies and amplitudes, which, when plotted in terms of t-values, result in the so-called \"SEP profiles\", did not demonstrate any significant alterations after placebo. Contrary to this, cyproterone acetate induced systematic and significant changes characterized by a latency increase in the early peaks and latency decrease in the late peaks of the SEP. Apart from the non-significant amplitude changes, such alterations were previously described by us as typical for drugs of the anxiolytic class. Mesterolone on the other hand, produced a significant latency decrease in the early part and a latency increase in the late part of the evoked response which was found to be typical for the SEP profiles of tricyclic antidepressants. The amplitude did not show any systematic changes. Based on step-wise discriminant analysis of these data we could significantly differentiate both hormones from placebo as well as from each other. A comparative analysis of low and high doses did not yield any significant differences between the two levels. It was concluded that both test substances have psychoactive properties; whereas cyproterone acetate reveals anxiolytic qualities, mesterolone exhibits antidepressant ones. These findings are discussed from the clinical as well as from the neurophysiological point of view.", "contents": "Are hormones psychoactive? Evoked potential investigations in man. The somatosensory evoked potential (SEP) of physically and mentally healthy male subjects was recorded before as well as 4 hours after administration of one single dose of placebo, cyproterone acetate (an antiandrogen), and mesterolone (an androgen). Quantitative evaluation of drug-induced changes in SEP latencies and amplitudes, which, when plotted in terms of t-values, result in the so-called \"SEP profiles\", did not demonstrate any significant alterations after placebo. Contrary to this, cyproterone acetate induced systematic and significant changes characterized by a latency increase in the early peaks and latency decrease in the late peaks of the SEP. Apart from the non-significant amplitude changes, such alterations were previously described by us as typical for drugs of the anxiolytic class. Mesterolone on the other hand, produced a significant latency decrease in the early part and a latency increase in the late part of the evoked response which was found to be typical for the SEP profiles of tricyclic antidepressants. The amplitude did not show any systematic changes. Based on step-wise discriminant analysis of these data we could significantly differentiate both hormones from placebo as well as from each other. A comparative analysis of low and high doses did not yield any significant differences between the two levels. It was concluded that both test substances have psychoactive properties; whereas cyproterone acetate reveals anxiolytic qualities, mesterolone exhibits antidepressant ones. These findings are discussed from the clinical as well as from the neurophysiological point of view."}
{"id": "PMID:1101914", "title": "New approaches to the classification of the lymphomata.", "content": "Our recently proposed functional approach to and classification of the malignant lymphomata based upon the T and B cell systems, lymphocyte transformation and their development as blocks or a \"switch on\" in lymphocyte transformation has been reviewed. This functional classification contains 5 major groups: (a) U cell or undefined for those proliferations without specific markers; (b) T cell; (c) B cell; (d) histiocytes as macrophages; (e) unclassifiable for those technically insufficient for specific cytological classification. Retrospective study of several case populations indicates that the majority of non-Hodgkin's lymphomata exhibit features of follicular centre cell (FCC) lymphomata of either cleaved or non-cleaved types. The prognostically favourable status of nodular lymphomata seems to result from the retained capability of follicle formation by FCC types with limited abnormality. Lymphomata of large cell types previously classified as histiocytic lymphoma or reticulum cell sarcoma predominantly resemble transformed lymphocytes and rarely exhibit features of histiocytes as macrophages. They are designated \"immunoblastic sarcoma\" when they occur as large transformed lymphocytes and may have either T or B cell immunological markers. Immunoblastic sarcoma has been observed to develop in individuals with chronic abnormal immune states, Sj\u00f6gren's syndrome, alpha chain disease, in patients on immunosuppression therapy for graft rejection and in senescence. There is accumulating evidence of lymphomata of T cells but none are firmly established. These include S\u00e9zary's syndrome, mycosis fungoides and the convoluted lymphocyte type, prev-ously included designated as acute lymphocytic leukaemia with mediatinal mass. The results of initial functional studies provide support for the proposed classification and indicate that the modern pathological investigative approach requires the collection of fresh lymphomatous tissue and an integrated immunocytochemical and morphological approach for the precise characterization of human lymphoma cell types.", "contents": "New approaches to the classification of the lymphomata. Our recently proposed functional approach to and classification of the malignant lymphomata based upon the T and B cell systems, lymphocyte transformation and their development as blocks or a \"switch on\" in lymphocyte transformation has been reviewed. This functional classification contains 5 major groups: (a) U cell or undefined for those proliferations without specific markers; (b) T cell; (c) B cell; (d) histiocytes as macrophages; (e) unclassifiable for those technically insufficient for specific cytological classification. Retrospective study of several case populations indicates that the majority of non-Hodgkin's lymphomata exhibit features of follicular centre cell (FCC) lymphomata of either cleaved or non-cleaved types. The prognostically favourable status of nodular lymphomata seems to result from the retained capability of follicle formation by FCC types with limited abnormality. Lymphomata of large cell types previously classified as histiocytic lymphoma or reticulum cell sarcoma predominantly resemble transformed lymphocytes and rarely exhibit features of histiocytes as macrophages. They are designated \"immunoblastic sarcoma\" when they occur as large transformed lymphocytes and may have either T or B cell immunological markers. Immunoblastic sarcoma has been observed to develop in individuals with chronic abnormal immune states, Sj\u00f6gren's syndrome, alpha chain disease, in patients on immunosuppression therapy for graft rejection and in senescence. There is accumulating evidence of lymphomata of T cells but none are firmly established. These include S\u00e9zary's syndrome, mycosis fungoides and the convoluted lymphocyte type, prev-ously included designated as acute lymphocytic leukaemia with mediatinal mass. The results of initial functional studies provide support for the proposed classification and indicate that the modern pathological investigative approach requires the collection of fresh lymphomatous tissue and an integrated immunocytochemical and morphological approach for the precise characterization of human lymphoma cell types."}
{"id": "PMID:1101915", "title": "Antigenic analysis of the lymphomata.", "content": "Soluble protein extracts of non-Hodgkin's lymphomata are fractionated by Sepharose chromatography. Each protein segregate is tested as a target antigen in immunoelectrophoresis using antiserum prepared by immunization with each fraction and an F and S antiserum of Hodgkin's tumour. F and S antigens are prominent in a histiocytic lymphoma with lymphoid infiltrate and Hodgkin's disease. A monospecific F antiserum is used to determine the stromal distribution of the antigen by immunofluorescence in tumour infiltrates and also to demonstrate F antigen activity in the medulla of the adolescent thymus. Implications of these findings are discussed and a general plan for analysis of the lymphomata outlined.", "contents": "Antigenic analysis of the lymphomata. Soluble protein extracts of non-Hodgkin's lymphomata are fractionated by Sepharose chromatography. Each protein segregate is tested as a target antigen in immunoelectrophoresis using antiserum prepared by immunization with each fraction and an F and S antiserum of Hodgkin's tumour. F and S antigens are prominent in a histiocytic lymphoma with lymphoid infiltrate and Hodgkin's disease. A monospecific F antiserum is used to determine the stromal distribution of the antigen by immunofluorescence in tumour infiltrates and also to demonstrate F antigen activity in the medulla of the adolescent thymus. Implications of these findings are discussed and a general plan for analysis of the lymphomata outlined."}
{"id": "PMID:1101916", "title": "Kinetic studies of circulating lymphocytes in malignant lymphatic disease using the impulse cytophotometer.", "content": "Measurements of the DNA content of mononuclear cells in the blood of patients with malignant lymphatic disease were made. In one case of acute lymphoblastic leukaemia, a large fraction of large type of blasts were in S phase. In the blood of a patient with leukaemic transformation of lymphosarcoma, a population of aneuploid tumour cells with a high percentage of cells in S phase was recognized. The increase of DNA synthesizing cells in the blood of patients with malignant lymphoma was associated with activity of the disease. The fraction of DNA synthesizing cells was raised sharply during chemotherapy.", "contents": "Kinetic studies of circulating lymphocytes in malignant lymphatic disease using the impulse cytophotometer. Measurements of the DNA content of mononuclear cells in the blood of patients with malignant lymphatic disease were made. In one case of acute lymphoblastic leukaemia, a large fraction of large type of blasts were in S phase. In the blood of a patient with leukaemic transformation of lymphosarcoma, a population of aneuploid tumour cells with a high percentage of cells in S phase was recognized. The increase of DNA synthesizing cells in the blood of patients with malignant lymphoma was associated with activity of the disease. The fraction of DNA synthesizing cells was raised sharply during chemotherapy."}
{"id": "PMID:1101917", "title": "Non-Hodgkin's lymphomata: clinical and immunological data in relation to histology.", "content": "Two hundred and forty-four previously untreated patients seen since 1964 in the department of haematology at Saint-Louis hospital were analysed. Clinical data included results of initial work-up and prognosis evaluated by survival rate matched with principal variables: age, sex, histopathology, staging, incidence of clinical and biological systemic symptoms. Immunological data included results of systematic studies made in each category of non-Hodgkin's lymphomata. Hyperbasophilic lymphoma, a special group recently characterized, is discussed.", "contents": "Non-Hodgkin's lymphomata: clinical and immunological data in relation to histology. Two hundred and forty-four previously untreated patients seen since 1964 in the department of haematology at Saint-Louis hospital were analysed. Clinical data included results of initial work-up and prognosis evaluated by survival rate matched with principal variables: age, sex, histopathology, staging, incidence of clinical and biological systemic symptoms. Immunological data included results of systematic studies made in each category of non-Hodgkin's lymphomata. Hyperbasophilic lymphoma, a special group recently characterized, is discussed."}
{"id": "PMID:1101918", "title": "Non-Hodgkin's lymphomata: clinical features in relation to histology.", "content": "An analysis is given of the clinico-pathological correlations of non-Hodgkin's lymphomata in 332 patients referred to the Netherlands Cancer Institute in Amsterdam and the Rotterdam Radiotherapy Institute. Clinical staging proved to be an important prognostic index. In Stage I the 5 year survival was 55%, in Stage II 25% and in Stages III and IV less than 10%. The presence of follicular structures in non-Hodgkin's lymphomata has similarly an important prognostic significance especially in Stage I and II. In lymphocytic lymphomata a larger cell size is correlated with less favourable prognosis. The presence of macrophages in non-Hodgkin's lymphomata is found in patients with short survival. The histiocytic lymphomata have a different survival pattern from the lymphocytic lymphomata in Stages I and II.", "contents": "Non-Hodgkin's lymphomata: clinical features in relation to histology. An analysis is given of the clinico-pathological correlations of non-Hodgkin's lymphomata in 332 patients referred to the Netherlands Cancer Institute in Amsterdam and the Rotterdam Radiotherapy Institute. Clinical staging proved to be an important prognostic index. In Stage I the 5 year survival was 55%, in Stage II 25% and in Stages III and IV less than 10%. The presence of follicular structures in non-Hodgkin's lymphomata has similarly an important prognostic significance especially in Stage I and II. In lymphocytic lymphomata a larger cell size is correlated with less favourable prognosis. The presence of macrophages in non-Hodgkin's lymphomata is found in patients with short survival. The histiocytic lymphomata have a different survival pattern from the lymphocytic lymphomata in Stages I and II."}
{"id": "PMID:1101919", "title": "A clinicopathological study of stages I and II non-Hodgkin's lymphomata using the Lukes-Collins classification.", "content": "A series of 226 patients with Stages I and II non-Hodgkin's lymphomata, treated with intensive irradiation to the involved regions, were studied using the Lukes-Collins classification. A statistically significant difference was found in the length of survival of patients with the follicular and the diffuse types of lymphoma. A difference in survival was also observed among those with the 3 subtypes of follicular lymphomata. The extent to which the follicular pattern could be recognized and whether or not lymphoma cells were confined to the follicular structures likewise had a bearing upon the survival of patients with follicular lymphomata. In this series, no significant difference in survival was found between the histological types of diffuse lymphoma. The relationship of the methods of staging to survival in this series, and the findings at staging laparotomy in other series suggest that the lymphangiogram is less valuable for detecting intra-abdominal disease in the non-Hodgkin's lymphomata than in Hodgkin's disease, probably because of the high incidence of involvement of mesenteric lymph nodes in the non-Hodgkin's lymphomata.", "contents": "A clinicopathological study of stages I and II non-Hodgkin's lymphomata using the Lukes-Collins classification. A series of 226 patients with Stages I and II non-Hodgkin's lymphomata, treated with intensive irradiation to the involved regions, were studied using the Lukes-Collins classification. A statistically significant difference was found in the length of survival of patients with the follicular and the diffuse types of lymphoma. A difference in survival was also observed among those with the 3 subtypes of follicular lymphomata. The extent to which the follicular pattern could be recognized and whether or not lymphoma cells were confined to the follicular structures likewise had a bearing upon the survival of patients with follicular lymphomata. In this series, no significant difference in survival was found between the histological types of diffuse lymphoma. The relationship of the methods of staging to survival in this series, and the findings at staging laparotomy in other series suggest that the lymphangiogram is less valuable for detecting intra-abdominal disease in the non-Hodgkin's lymphomata than in Hodgkin's disease, probably because of the high incidence of involvement of mesenteric lymph nodes in the non-Hodgkin's lymphomata."}
{"id": "PMID:1101920", "title": "Staging laparotomy in non-Hodgkin's lymphoma.", "content": "In 57 patients with non-Hodgkin's lymphoma, a clinical, radiographic, scintigraphic and pathological correlative study showed the following results: (1) the inferior venacavagram, lymphangiogram and gallium-67 scan have a low sensitivity in detecting lymphoma: their accuracy is high when the findings are interpreted as abnormal (93%, 83% and 80% respectively), but low when they are interpreted as normal (47%, 67% and 58% respectively); (2) the clinical evaluation of spleen and liver is unreliable; (3) the incidence of lymphocytic lymphoma in the para-aortic-iliac nodes is high; (4) a pattern of involvement by contiguity and a predilection for the spleen were observed in lymphocytic lymphoma; (5) in lymphocytic lymphoma there is no liver involve without concomitant splenic involvement; (6) no definite pattern of spread could be seen in histiocytic lymphoma; (7) surgical staging changed the classification of the lymphoma in 56% of cases, 46% being reclassified to a more advanced stage; (8) surgical staging significantly improves the assessment of the stage of disease and therefore permits accurate treatment planning.", "contents": "Staging laparotomy in non-Hodgkin's lymphoma. In 57 patients with non-Hodgkin's lymphoma, a clinical, radiographic, scintigraphic and pathological correlative study showed the following results: (1) the inferior venacavagram, lymphangiogram and gallium-67 scan have a low sensitivity in detecting lymphoma: their accuracy is high when the findings are interpreted as abnormal (93%, 83% and 80% respectively), but low when they are interpreted as normal (47%, 67% and 58% respectively); (2) the clinical evaluation of spleen and liver is unreliable; (3) the incidence of lymphocytic lymphoma in the para-aortic-iliac nodes is high; (4) a pattern of involvement by contiguity and a predilection for the spleen were observed in lymphocytic lymphoma; (5) in lymphocytic lymphoma there is no liver involve without concomitant splenic involvement; (6) no definite pattern of spread could be seen in histiocytic lymphoma; (7) surgical staging changed the classification of the lymphoma in 56% of cases, 46% being reclassified to a more advanced stage; (8) surgical staging significantly improves the assessment of the stage of disease and therefore permits accurate treatment planning."}
{"id": "PMID:1101921", "title": "Staging laparotomy in non-Hodgkin's lymphomata.", "content": "Staging laparotomy was performed in 106 selected untreated adults and in 6 children. In adults the histological pattern at initial biopsy was interpreted as nodular lymphoma in 33% and as diffuse lymphoma in 67%. Diffuse histiocytic was the most frequently observed histological subtype in the present series (54%). Diffuse undifferentiated lymphoma was noted in 2/6 children. Waldeyer's ring involvement was noted in 27% of adult patients. Systemic symptoms were present in 7%. Surgery detected occult lesions in 27% of patients (para-aortic nodes 3%, spleen 12%, liver 12%, coeliac nodes 15%, splenic hilar nodes 26%, mesenteric nodes 15%, gastrointestinal tract 1%, bone marrow 12%). The chance of detecting splenic and hepatic involvement was definitely higher in patients with nodal disease above and below the diaphragm in comparison with those with either supra-diaphragmatic or infra-diaphragmatic adenopathy. Lymphography yielded a 96% accuracy proving once more to be a sufficiently reliable diagnostic method. Staging laparotomy is a useful procedure in non-Hodgkin's lymphomata in order to gain information on the natural history and to plan treatment. It remains to be determined whether findings observed through surgical staging will lead to improved treatment and survival.", "contents": "Staging laparotomy in non-Hodgkin's lymphomata. Staging laparotomy was performed in 106 selected untreated adults and in 6 children. In adults the histological pattern at initial biopsy was interpreted as nodular lymphoma in 33% and as diffuse lymphoma in 67%. Diffuse histiocytic was the most frequently observed histological subtype in the present series (54%). Diffuse undifferentiated lymphoma was noted in 2/6 children. Waldeyer's ring involvement was noted in 27% of adult patients. Systemic symptoms were present in 7%. Surgery detected occult lesions in 27% of patients (para-aortic nodes 3%, spleen 12%, liver 12%, coeliac nodes 15%, splenic hilar nodes 26%, mesenteric nodes 15%, gastrointestinal tract 1%, bone marrow 12%). The chance of detecting splenic and hepatic involvement was definitely higher in patients with nodal disease above and below the diaphragm in comparison with those with either supra-diaphragmatic or infra-diaphragmatic adenopathy. Lymphography yielded a 96% accuracy proving once more to be a sufficiently reliable diagnostic method. Staging laparotomy is a useful procedure in non-Hodgkin's lymphomata in order to gain information on the natural history and to plan treatment. It remains to be determined whether findings observed through surgical staging will lead to improved treatment and survival."}
{"id": "PMID:1101922", "title": "Spread according to histology in non-Hodgkin's lymphomata.", "content": "The spread of non-Hodgkin's lymphomata was investigated in a group of 323 cases. Differences were sought between 4 histological groups: lymphocytic vs histiocytic lymphomata and diffuse vs follicular types. Histiocytic and follicular types were rare in childhood. Histiocytic lymphomata, compared with lymphocytic ones, were more often confined to sites above the diaphragm. They exhibited a higher rate of loco-regional recurrences within the first 3 months and also of late recurrence after 4 years. Lymphocytic lymphomata of the follicular type more commonly presented with nodal involvement below the diaphragm as primary or secondary site than the diffuse form. Follicular types of lymphoma compared with diffuse types, occurred more frequently in women. They were more seldom seen as primary or secondary sites in extranodal localizations. They had a smaller chance of late disease recurrence and were associated with a longer survival time after first recurrence. The mediastinum often remained clinically uninvolved. With regard to this mediastinal \"skip\" no differences were found between the histological groups.", "contents": "Spread according to histology in non-Hodgkin's lymphomata. The spread of non-Hodgkin's lymphomata was investigated in a group of 323 cases. Differences were sought between 4 histological groups: lymphocytic vs histiocytic lymphomata and diffuse vs follicular types. Histiocytic and follicular types were rare in childhood. Histiocytic lymphomata, compared with lymphocytic ones, were more often confined to sites above the diaphragm. They exhibited a higher rate of loco-regional recurrences within the first 3 months and also of late recurrence after 4 years. Lymphocytic lymphomata of the follicular type more commonly presented with nodal involvement below the diaphragm as primary or secondary site than the diffuse form. Follicular types of lymphoma compared with diffuse types, occurred more frequently in women. They were more seldom seen as primary or secondary sites in extranodal localizations. They had a smaller chance of late disease recurrence and were associated with a longer survival time after first recurrence. The mediastinum often remained clinically uninvolved. With regard to this mediastinal \"skip\" no differences were found between the histological groups."}
{"id": "PMID:1101923", "title": "The natural history of non-Hodgkin's lymphomata stages I and II.", "content": "Progress in the management of non-Hodgkin's lymphomata has been impeded by lack of information on the natural history of these diseases. Confusion about the significance of histopathology, extranodal presentations and routes of spread renders much of previously published data relatively uninterpretable. To evaluate the relative prognostic significance of presentation, histopathology and lymphography, a retrospective study was undertaken on 226 patients with Stage I and II disease, who were treated with intensive irradiation to the involve regions. The slides on these patients were reviewed and reclassified in terms of the Rappaport system. The results of this study demonstrated that a nodular histopathological pattern was the most important prognostic factor. The significance of this finding and other factors are discussed in terms of possible new approaches to treatment.", "contents": "The natural history of non-Hodgkin's lymphomata stages I and II. Progress in the management of non-Hodgkin's lymphomata has been impeded by lack of information on the natural history of these diseases. Confusion about the significance of histopathology, extranodal presentations and routes of spread renders much of previously published data relatively uninterpretable. To evaluate the relative prognostic significance of presentation, histopathology and lymphography, a retrospective study was undertaken on 226 patients with Stage I and II disease, who were treated with intensive irradiation to the involve regions. The slides on these patients were reviewed and reclassified in terms of the Rappaport system. The results of this study demonstrated that a nodular histopathological pattern was the most important prognostic factor. The significance of this finding and other factors are discussed in terms of possible new approaches to treatment."}
{"id": "PMID:1101924", "title": "Non-Hodgkin's lymphoma in children.", "content": "The clinical and pathological features of 64 children with non-Hodgkin's malignant lymphoma seen between April 1962 and June 1973 are described. Forty-one children had diffuse, undifferentiated, non-Burkitt lymphoma (lymphoblastic lymphoma). They tended to be boys under 10 years of age and their median survival was 1 year. Almost one-third are surviving for 1-11 years, most in initial complete remission. Nineteen children had diffuse, poorly differentiated, histiocytic lymphoma. They tended to be boys more than 10 years of age, their median survival was only 6 months, and only the 3 patients with Stage I peripheral node tumour survived. Two children had nodular, lymphocytic, poorly differentiated lymphoma and 2 had lymphoma resembling the Burkitt type. From our clinical and pathological observations, we conclude that non-Hodgkin's malignant lymphomata in children cannot be separated from the acute lymphocytic, histiocytic and unclassified leukaemias by cytological or histological methods. What is called diffuse, undifferentiated, non-Burkitt type, or lymphoblastic lymphoma is actually acute lymphocytic leukaemia without apparent invasion of marrow and peripheral blood by neoplastic lymphocytes at time of diagnosis. What is termed diffuse, histiocytic lymphoma is acute histiocytic leukaemia without apparent infiltration of marrow and peripheral blood at initial presentation. One could say just as well that acute lymphocytic leukaemia is Stage IV lymphoblastic lymphoma and that acute histiocytic leukaemia is Stage IV histiocytic lymphoma. Further classification of lymphocytic and histiocytic cancers by newer functional, chemical and morphological methods should include both what is called lymphocytic or histiocytic leukaemia and what is called non-Hodgkin's lymphoma as one group of diseases, susceptible to subclassification by the new methods. We recommend that Stage I lymphocytic and histiocytic cancers be treated with local irradiation. Patients with Stages II-IV tumours should receive anti-leukaemic forms of therapy including prolonged multiple agent chemotherapy and preventive central nervous system irradiation. Staging laparotomy should be considered in patients with Stage I tumour in low cervical, axillary and inguinal nodes.", "contents": "Non-Hodgkin's lymphoma in children. The clinical and pathological features of 64 children with non-Hodgkin's malignant lymphoma seen between April 1962 and June 1973 are described. Forty-one children had diffuse, undifferentiated, non-Burkitt lymphoma (lymphoblastic lymphoma). They tended to be boys under 10 years of age and their median survival was 1 year. Almost one-third are surviving for 1-11 years, most in initial complete remission. Nineteen children had diffuse, poorly differentiated, histiocytic lymphoma. They tended to be boys more than 10 years of age, their median survival was only 6 months, and only the 3 patients with Stage I peripheral node tumour survived. Two children had nodular, lymphocytic, poorly differentiated lymphoma and 2 had lymphoma resembling the Burkitt type. From our clinical and pathological observations, we conclude that non-Hodgkin's malignant lymphomata in children cannot be separated from the acute lymphocytic, histiocytic and unclassified leukaemias by cytological or histological methods. What is called diffuse, undifferentiated, non-Burkitt type, or lymphoblastic lymphoma is actually acute lymphocytic leukaemia without apparent invasion of marrow and peripheral blood by neoplastic lymphocytes at time of diagnosis. What is termed diffuse, histiocytic lymphoma is acute histiocytic leukaemia without apparent infiltration of marrow and peripheral blood at initial presentation. One could say just as well that acute lymphocytic leukaemia is Stage IV lymphoblastic lymphoma and that acute histiocytic leukaemia is Stage IV histiocytic lymphoma. Further classification of lymphocytic and histiocytic cancers by newer functional, chemical and morphological methods should include both what is called lymphocytic or histiocytic leukaemia and what is called non-Hodgkin's lymphoma as one group of diseases, susceptible to subclassification by the new methods. We recommend that Stage I lymphocytic and histiocytic cancers be treated with local irradiation. Patients with Stages II-IV tumours should receive anti-leukaemic forms of therapy including prolonged multiple agent chemotherapy and preventive central nervous system irradiation. Staging laparotomy should be considered in patients with Stage I tumour in low cervical, axillary and inguinal nodes."}
{"id": "PMID:1101926", "title": "A clinico-pathological study of non-Hodgkin's lymphomata in childhood.", "content": "In a series of 27 children with non-Hodgkin's lymphomata during a 29-year period there were 21 boys and 6 girls. Thirteen had lymphocytic lymphoma, 9 histiocytic and 5 lymphoma with a prominent starry-sky appearance. All but one had diffuse lymphoma and no case of mixed cell type lymphoma was seen. Patients with lymphocytic lymphoma had extralymphatic manifestations more often than patients with the other types, and leukaemic transformation, which was seen in 6 patients, was especially common in lymphocytic lymphoma. Patients with Burkitt-type tumours did not respond well to radiation therapy with conventional fractionation. The survival in the present series was very poor, only 3 out of 27 children still being alive. A more aggressive therapeutic approach, guided by a differentiated microscopic classification and appropriate staging procedures, may be expected to give better results.", "contents": "A clinico-pathological study of non-Hodgkin's lymphomata in childhood. In a series of 27 children with non-Hodgkin's lymphomata during a 29-year period there were 21 boys and 6 girls. Thirteen had lymphocytic lymphoma, 9 histiocytic and 5 lymphoma with a prominent starry-sky appearance. All but one had diffuse lymphoma and no case of mixed cell type lymphoma was seen. Patients with lymphocytic lymphoma had extralymphatic manifestations more often than patients with the other types, and leukaemic transformation, which was seen in 6 patients, was especially common in lymphocytic lymphoma. Patients with Burkitt-type tumours did not respond well to radiation therapy with conventional fractionation. The survival in the present series was very poor, only 3 out of 27 children still being alive. A more aggressive therapeutic approach, guided by a differentiated microscopic classification and appropriate staging procedures, may be expected to give better results."}
{"id": "PMID:1101929", "title": "Prognostic significance of primary site after radiotherapy in non-Hodgkin's lymphomata.", "content": "In contrast to Hodgkin's lymphomata, non-Hodgkin's lymphomata originate in approximately a fourth of the cases in extranodal organs and sites. The prognosis of patients with primary extranodal organ involvement of the non-Hodgkin's lymphomata is similar to that of patients with primary lumph node involvement of the Hodgkin's and non-Hodgkin's lymphomata, dependent on the special spreading of the disease corresponding to the 4 stages of the Ann Arbor classification. This applies to the primary involvement of a single lymph node region (Stage I) or a single extralymphatic organ or site (stage IE) as well as to further stages of spreading within the lymphatic system (Stages II and III) including secondary localized involvement of an extralymphatic organ and site (Stages IIE and IIIE). The same qualitative dependence of the prognosis of Hodgkin's and non-Hodgkin's lymphomata on the spatial spreading, corresponding to the Ann Arbor concept, legitimizes, in spite of some quantitative differences, the application of the Ann Arbor classification system to all malignant lymphomata.", "contents": "Prognostic significance of primary site after radiotherapy in non-Hodgkin's lymphomata. In contrast to Hodgkin's lymphomata, non-Hodgkin's lymphomata originate in approximately a fourth of the cases in extranodal organs and sites. The prognosis of patients with primary extranodal organ involvement of the non-Hodgkin's lymphomata is similar to that of patients with primary lumph node involvement of the Hodgkin's and non-Hodgkin's lymphomata, dependent on the special spreading of the disease corresponding to the 4 stages of the Ann Arbor classification. This applies to the primary involvement of a single lymph node region (Stage I) or a single extralymphatic organ or site (stage IE) as well as to further stages of spreading within the lymphatic system (Stages II and III) including secondary localized involvement of an extralymphatic organ and site (Stages IIE and IIIE). The same qualitative dependence of the prognosis of Hodgkin's and non-Hodgkin's lymphomata on the spatial spreading, corresponding to the Ann Arbor concept, legitimizes, in spite of some quantitative differences, the application of the Ann Arbor classification system to all malignant lymphomata."}
{"id": "PMID:1101930", "title": "Sclerosis in non-Hodgkin's lymphomata.", "content": "A total of 242 cases of non-Hodgkin's lymphoma arising primarily in lymph nodes are reported. Lymph nodes have been classified histologically and the incidence of sclerosis in each group assessed. Two types of fibrosis were found, one composed of fibrous bands from 10 mum to several hundred mum thick within the node and invaded perinodal tissue. The other type was a fine compartmentalizing fibrosis sometimes simulating the pattern of metastatic carcinoma in part of a node, the rest of the node being typical of malignant lymphoma. Both types of fibrosis were present in some nodes. Fibrous bands were present in all histological types, with the exception of the diffuse lymphocytic well differentiated group. Fine compartmentalizing fibrosis was seen only in association with areas of undifferentiated large celled lymphoma. The presence of sclerosis is shown to correlate with a better prognosis when compared with non-sclerosing tymours of similar histological type. That sclerosing lymphomata are more slowly progressive is borne out by the fact that two-thirds of the patients with sclerotic nodes presented with localized disease compared with less than half of the patients whose nodes showed no sclerosis.", "contents": "Sclerosis in non-Hodgkin's lymphomata. A total of 242 cases of non-Hodgkin's lymphoma arising primarily in lymph nodes are reported. Lymph nodes have been classified histologically and the incidence of sclerosis in each group assessed. Two types of fibrosis were found, one composed of fibrous bands from 10 mum to several hundred mum thick within the node and invaded perinodal tissue. The other type was a fine compartmentalizing fibrosis sometimes simulating the pattern of metastatic carcinoma in part of a node, the rest of the node being typical of malignant lymphoma. Both types of fibrosis were present in some nodes. Fibrous bands were present in all histological types, with the exception of the diffuse lymphocytic well differentiated group. Fine compartmentalizing fibrosis was seen only in association with areas of undifferentiated large celled lymphoma. The presence of sclerosis is shown to correlate with a better prognosis when compared with non-sclerosing tymours of similar histological type. That sclerosing lymphomata are more slowly progressive is borne out by the fact that two-thirds of the patients with sclerotic nodes presented with localized disease compared with less than half of the patients whose nodes showed no sclerosis."}
{"id": "PMID:1101931", "title": "Clinical trials in the non-Hodgkin's lymphomata at Stanford University experimental design and preliminary results.", "content": "Preliminary results of controlled clinical trials in 127 patients with non-Hodgkin's lymphomata are reported. The rationale for identifying 7 study groups is presented. Treatment programmes which are being studied vary from involved field irradiation to the most aggressive treatment tolerable. The early results suggest that patients with both favourable and unfavourable histologies but of limited extent can be controlled with irradiation. Patients with advanced disease but with favourable histological subgroups are doing as well with a conservative drug programme as with 2 aggressive treatment regimens. These controlled studies and those of others, facilitated by the use of the Rappaport classification and laparotomy staging, give promise of clarifying and improving the management of patients with these lymphomata.", "contents": "Clinical trials in the non-Hodgkin's lymphomata at Stanford University experimental design and preliminary results. Preliminary results of controlled clinical trials in 127 patients with non-Hodgkin's lymphomata are reported. The rationale for identifying 7 study groups is presented. Treatment programmes which are being studied vary from involved field irradiation to the most aggressive treatment tolerable. The early results suggest that patients with both favourable and unfavourable histologies but of limited extent can be controlled with irradiation. Patients with advanced disease but with favourable histological subgroups are doing as well with a conservative drug programme as with 2 aggressive treatment regimens. These controlled studies and those of others, facilitated by the use of the Rappaport classification and laparotomy staging, give promise of clarifying and improving the management of patients with these lymphomata."}
{"id": "PMID:1101932", "title": "Results of combination chemotherapy of non-Hodgkin's lymphoma.", "content": "The analysis of the results of CVP and MOPP chemotherapy in 80 patients with advanced stages of non-Hodgkin's lymphoma shows that 36 achieved a complete remission. Twenty-five percent of all patients remain free of disease for periods ranging from 4 months to over 7 years, with a projected median duration of complete remissions of 3 1/2 years. Well differentiated and nodular histology were positive determinants for survival, confirming the overall clinical validity of the Rappaport classification system for the non-Hodgkin's lymphomata. However, it has been demonstrated that it is possible to achieve a complete remission using combination chemotherapy even in the most clinically aggressive histological subgroups, and that these responses can be correlated with an extended survival. An analysis of patterns of relapse from complete remission in patients with non-Hodgkin's lymphoma treated with combination chemotherapy has demonstrated differences between histological subgroups. Patients with diffuse histiocytic lymphoma who achieved a complete remission after 6 months of treatment without maintenance have remained disease-free, whereas those with nodular poorly differentiated lymphocytic and diffuse well differentiated lymphocytic lymphomata demonstrate a pattern of continuous late recurrence. The initial sites of relapse from complete remission in lymphocytic lymphoma were lymph nodes and bone marrow which were involved before treatment.", "contents": "Results of combination chemotherapy of non-Hodgkin's lymphoma. The analysis of the results of CVP and MOPP chemotherapy in 80 patients with advanced stages of non-Hodgkin's lymphoma shows that 36 achieved a complete remission. Twenty-five percent of all patients remain free of disease for periods ranging from 4 months to over 7 years, with a projected median duration of complete remissions of 3 1/2 years. Well differentiated and nodular histology were positive determinants for survival, confirming the overall clinical validity of the Rappaport classification system for the non-Hodgkin's lymphomata. However, it has been demonstrated that it is possible to achieve a complete remission using combination chemotherapy even in the most clinically aggressive histological subgroups, and that these responses can be correlated with an extended survival. An analysis of patterns of relapse from complete remission in patients with non-Hodgkin's lymphoma treated with combination chemotherapy has demonstrated differences between histological subgroups. Patients with diffuse histiocytic lymphoma who achieved a complete remission after 6 months of treatment without maintenance have remained disease-free, whereas those with nodular poorly differentiated lymphocytic and diffuse well differentiated lymphocytic lymphomata demonstrate a pattern of continuous late recurrence. The initial sites of relapse from complete remission in lymphocytic lymphoma were lymph nodes and bone marrow which were involved before treatment."}
{"id": "PMID:1101933", "title": "Therapy of advanced lymphocytic lymphoma a preliminary report of a randomized trial between combination chemotherapy (CVP) and intensive radiotherapy.", "content": "The initial results of a randomized clinical trial comparing intensive cyclical combination chemotherapy (CVP) and total body radiation therapy in the treatment of advanced (Stage III and IV) lymphocytic lymphoma is presented. Sixty-five patients have been entered and randomized according to stage. Of the chemotherapy treated patients, (22/27 (81%) achieved a response with 55% complete responders. The radiation group or those treated with radiation 27/32 (84%) responded with 56% in complete response. There is no significant difference in overall survival between the two groups. Nodular lymphoma (44 patients) responds more often and has s significantly longer survival than diffuse lymphoma (21 patients). Approximately 50% of the complete remitters have relapsed but reinduction of disease control was possible in almost all cases.", "contents": "Therapy of advanced lymphocytic lymphoma a preliminary report of a randomized trial between combination chemotherapy (CVP) and intensive radiotherapy. The initial results of a randomized clinical trial comparing intensive cyclical combination chemotherapy (CVP) and total body radiation therapy in the treatment of advanced (Stage III and IV) lymphocytic lymphoma is presented. Sixty-five patients have been entered and randomized according to stage. Of the chemotherapy treated patients, (22/27 (81%) achieved a response with 55% complete responders. The radiation group or those treated with radiation 27/32 (84%) responded with 56% in complete response. There is no significant difference in overall survival between the two groups. Nodular lymphoma (44 patients) responds more often and has s significantly longer survival than diffuse lymphoma (21 patients). Approximately 50% of the complete remitters have relapsed but reinduction of disease control was possible in almost all cases."}
{"id": "PMID:1101934", "title": "The influence of chemotherapy on the management of non-Hodgkin's lymphomata at the Princess Margaret Hospital. A comparison of the results from 1962-64 with 1967-69.", "content": "A retrospective study of 460 non-Hodgkin's lymphoma patients seen in 1962-64 (185) and 1967-69 (275) revealed a marked improvement in the survival of patients treated during the later interval. The increased survival was not due to differences in the age or sex distribution of patients, the proportions with systemic symptoms or extranodal presentations. The improvement was due partially to a disproportionate increase in the number of patients with well- and intermediately differentiated nodular lymphocytic lymphomata in 1967-69. When these patients  were removed, the proportions of patients with \"good\" and \"poor\" pathology were comparable for the two intervals, and a markedly improved survival was still evident for the 1967-69 group. An improvement in the proportion of patients achieving a complete remission occurred only in patients who received some form of chemotherapy. Relapses occurred in 44.6% of patients with Stage I and II disease treated with radiotherapy alone. The results of this study indicate that we must re-evaluate carefully the indications for radiotherapy in the treatment of the non-Hodgkin's lymphomata. The addition of chemotherapy appears to be a promising method for increasing the proportion of patients who will achieve a long complete remission.", "contents": "The influence of chemotherapy on the management of non-Hodgkin's lymphomata at the Princess Margaret Hospital. A comparison of the results from 1962-64 with 1967-69. A retrospective study of 460 non-Hodgkin's lymphoma patients seen in 1962-64 (185) and 1967-69 (275) revealed a marked improvement in the survival of patients treated during the later interval. The increased survival was not due to differences in the age or sex distribution of patients, the proportions with systemic symptoms or extranodal presentations. The improvement was due partially to a disproportionate increase in the number of patients with well- and intermediately differentiated nodular lymphocytic lymphomata in 1967-69. When these patients  were removed, the proportions of patients with \"good\" and \"poor\" pathology were comparable for the two intervals, and a markedly improved survival was still evident for the 1967-69 group. An improvement in the proportion of patients achieving a complete remission occurred only in patients who received some form of chemotherapy. Relapses occurred in 44.6% of patients with Stage I and II disease treated with radiotherapy alone. The results of this study indicate that we must re-evaluate carefully the indications for radiotherapy in the treatment of the non-Hodgkin's lymphomata. The addition of chemotherapy appears to be a promising method for increasing the proportion of patients who will achieve a long complete remission."}
{"id": "PMID:1101935", "title": "Cytology in the classification of diffuse non-leukaemic malignant lymphomata (lympho- and reticulosarcomata).", "content": "Cytological examination of smears or imprints of diffuse non-leukaemic lymphomata gives more details of the morphological aspects of the cells than does histological examination. It enables us to distinguish (a) prolymphocytic (but not lymphocytic), (b) lymphoblastic or lymphoblastoid and immunoblastic lymphosarcomata. It helps to diagnose so-called reticulosarcomata from carcinomata and to distinguish two types: (a) cytic and (b) blastic, but it makes us suspicious about the nature of the latter. Light microscopy shows the cells of the blastic type resemble transformed lymphocytes (immunoblasts) more than reticulum cells. Electron microscopy shows many polyribosomes, which enhances the suspicion that some so-called \"reticulosarcomata\" could be immunoblastic lymphosarcomata.", "contents": "Cytology in the classification of diffuse non-leukaemic malignant lymphomata (lympho- and reticulosarcomata). Cytological examination of smears or imprints of diffuse non-leukaemic lymphomata gives more details of the morphological aspects of the cells than does histological examination. It enables us to distinguish (a) prolymphocytic (but not lymphocytic), (b) lymphoblastic or lymphoblastoid and immunoblastic lymphosarcomata. It helps to diagnose so-called reticulosarcomata from carcinomata and to distinguish two types: (a) cytic and (b) blastic, but it makes us suspicious about the nature of the latter. Light microscopy shows the cells of the blastic type resemble transformed lymphocytes (immunoblasts) more than reticulum cells. Electron microscopy shows many polyribosomes, which enhances the suspicion that some so-called \"reticulosarcomata\" could be immunoblastic lymphosarcomata."}
{"id": "PMID:1101936", "title": "Follicular lymphoma: A survey of 75 cases with special reference to the syndrome resembling chronic lymphocytic leukaemia.", "content": "In a series of 75 cases of follicular lymphoma collected between 1950 and 1971 the following features were singled out as characteristic of, or specially frequent in, follicular lymphoma: (1) the high incidence in females; (2) the long history of symptomless lymph node enlargement; (3) the frequent presence of extensive symptomless disease at presentation, especially in patients below 40 years of age; (4) the syndrome resembling and often confused with chronic lymphocytic leukaemia, in which the notched nucleus cell, when present, is diagnostic; (5) the responsiveness of extensive disease to radiotherapy and alkylating agents at low dosage; (6) the long overall survival and the long survival after relapse; (7) the occurrence of abrupt change in the character of the disease in which, during a period of apparent quiescence, malignant transformation supervenes proving rapidly fatal. In the transformed stage the disease behaves as an invasive metastasizing sarcoma or as a blast cell leukaemia; in both cases the transformation is associated with the appearance of a new cell type, undifferentiated and distinct from that present before onset of transformation, which may however persist. The syndrome resembling chronic lymphocytic leukaemia occurs only in follicular lymphoma.", "contents": "Follicular lymphoma: A survey of 75 cases with special reference to the syndrome resembling chronic lymphocytic leukaemia. In a series of 75 cases of follicular lymphoma collected between 1950 and 1971 the following features were singled out as characteristic of, or specially frequent in, follicular lymphoma: (1) the high incidence in females; (2) the long history of symptomless lymph node enlargement; (3) the frequent presence of extensive symptomless disease at presentation, especially in patients below 40 years of age; (4) the syndrome resembling and often confused with chronic lymphocytic leukaemia, in which the notched nucleus cell, when present, is diagnostic; (5) the responsiveness of extensive disease to radiotherapy and alkylating agents at low dosage; (6) the long overall survival and the long survival after relapse; (7) the occurrence of abrupt change in the character of the disease in which, during a period of apparent quiescence, malignant transformation supervenes proving rapidly fatal. In the transformed stage the disease behaves as an invasive metastasizing sarcoma or as a blast cell leukaemia; in both cases the transformation is associated with the appearance of a new cell type, undifferentiated and distinct from that present before onset of transformation, which may however persist. The syndrome resembling chronic lymphocytic leukaemia occurs only in follicular lymphoma."}
{"id": "PMID:1101937", "title": "Electron microscopy in the non-Hodgkin's lymphomata.", "content": "The component cells of peripheral lymphoid tissue have been divided into the lymphocyte and plasma cell lines, mononuclear phagocytic cells, dendritic \"reticular cells\", the reticular (supporting) cells and endothelial cells, and it is suggested that this system of cells should collectively be referred to as the lymphoreticular monoclear phagocyte system or LRMPS. Seventeen tumours of the LRMPS (excluding Hodgkin's disease) have been studied at ultrastructural level. Of these 17 non-Hodgkin lymphomata 5 were follicular lymphomata and 12 diffuse. It is concluded that electron microscopy plays a valuable role in the diagnosis of this group of tumours. Not only does it allow rejection of a diagnosis of lymphoma in certain anaplastic tumours, but it also enables a more precise identification of the cellular components of a lymphoma as well as indicating the degree of differentiation of the cell line involved. Additional advantages are the visualization of subcellular structures useful as markers, and by means of specialized immunoelectron microscopic techniques the identification of antigens and antibody formation within a given tumour. Two other results of this ultrastructural study are the indication that the dendritic cells of lymphoid follicles are derived from capillary endothelium, and the identification of certain anomalous formations derived from rough endoplasmic reticulum in the case of tumours showing plasmacytoid differentiation.", "contents": "Electron microscopy in the non-Hodgkin's lymphomata. The component cells of peripheral lymphoid tissue have been divided into the lymphocyte and plasma cell lines, mononuclear phagocytic cells, dendritic \"reticular cells\", the reticular (supporting) cells and endothelial cells, and it is suggested that this system of cells should collectively be referred to as the lymphoreticular monoclear phagocyte system or LRMPS. Seventeen tumours of the LRMPS (excluding Hodgkin's disease) have been studied at ultrastructural level. Of these 17 non-Hodgkin lymphomata 5 were follicular lymphomata and 12 diffuse. It is concluded that electron microscopy plays a valuable role in the diagnosis of this group of tumours. Not only does it allow rejection of a diagnosis of lymphoma in certain anaplastic tumours, but it also enables a more precise identification of the cellular components of a lymphoma as well as indicating the degree of differentiation of the cell line involved. Additional advantages are the visualization of subcellular structures useful as markers, and by means of specialized immunoelectron microscopic techniques the identification of antigens and antibody formation within a given tumour. Two other results of this ultrastructural study are the indication that the dendritic cells of lymphoid follicles are derived from capillary endothelium, and the identification of certain anomalous formations derived from rough endoplasmic reticulum in the case of tumours showing plasmacytoid differentiation."}
{"id": "PMID:1101938", "title": "Leukaemic conversion of non-Hodgkin's malignant lymphomata.", "content": "In 143 patients with poorly differentiated lymphosarcoma, leukaemic conversion has been observed in 25. The cytological type was prolymphocytic or lymphoblastic or lymphoblastoid (immunoblastic ?). Twenty-five patients were treated with chemo-radiotherapy, followed by active immunotherapy as if they had primary acute lymphoid leukaemia. A complete remission was obtained in 11. Four are still in first complete remission after 4 1/2 years. Among 136 patients suffering from so-called \"poorly differentiated reticulosarcoma\", 17 became leukaemic. The cells are cytologically very dystrophic and unidentifiable. A remission was obtained in 7 patients but it was of short duration (median 1 1/2 months, longest 7 months).", "contents": "Leukaemic conversion of non-Hodgkin's malignant lymphomata. In 143 patients with poorly differentiated lymphosarcoma, leukaemic conversion has been observed in 25. The cytological type was prolymphocytic or lymphoblastic or lymphoblastoid (immunoblastic ?). Twenty-five patients were treated with chemo-radiotherapy, followed by active immunotherapy as if they had primary acute lymphoid leukaemia. A complete remission was obtained in 11. Four are still in first complete remission after 4 1/2 years. Among 136 patients suffering from so-called \"poorly differentiated reticulosarcoma\", 17 became leukaemic. The cells are cytologically very dystrophic and unidentifiable. A remission was obtained in 7 patients but it was of short duration (median 1 1/2 months, longest 7 months)."}
{"id": "PMID:1101939", "title": "Humoral and cellular immunity in atopic eczema.", "content": "Parameters of humoral and cellular immunity were measured in thirty-five patients with atopic eczema. The mean serum IgE level was raised but levels of the other major immunoglobulin classes were normal. Ten per cent of patients failed to respond to tetanus immunization. All patients responded to S. typhi H antigen. Fourteen per cent of patients failed to mount delayed hypersensitivity reactions to a battery of three intradermal antigens. The phytohaemagglutinin-stimulated uptake of 3H thymidine by lymphocytes was normal in the presence of autologous or of fetal calf serum, as was the spontaneous lymphocyte uptake. T and B lymphocyte numbers in the peripheral blood were normal. These results are similar to those found in asthmatic patients and support the hypothesis that, in some patients, atopic eczema is associated with an immunodeficiency state.", "contents": "Humoral and cellular immunity in atopic eczema. Parameters of humoral and cellular immunity were measured in thirty-five patients with atopic eczema. The mean serum IgE level was raised but levels of the other major immunoglobulin classes were normal. Ten per cent of patients failed to respond to tetanus immunization. All patients responded to S. typhi H antigen. Fourteen per cent of patients failed to mount delayed hypersensitivity reactions to a battery of three intradermal antigens. The phytohaemagglutinin-stimulated uptake of 3H thymidine by lymphocytes was normal in the presence of autologous or of fetal calf serum, as was the spontaneous lymphocyte uptake. T and B lymphocyte numbers in the peripheral blood were normal. These results are similar to those found in asthmatic patients and support the hypothesis that, in some patients, atopic eczema is associated with an immunodeficiency state."}
{"id": "PMID:1101940", "title": "Viral warts, herpes simplex and herpes zoster in patients with secondary immune deficiencies and neoplasms.", "content": "The incidence of viral warts, recurrent herpes simplex and herpes zoster has been assessed in a group of patients with possible secondary immune deficiency states and compared to a control group. Patients with cell-mediated immune deficiency appear to be more prone to warts and zoster infection as compared to patients with humoral immune deficiency. Recurrent herpes simplex infection was not increased in either group.", "contents": "Viral warts, herpes simplex and herpes zoster in patients with secondary immune deficiencies and neoplasms. The incidence of viral warts, recurrent herpes simplex and herpes zoster has been assessed in a group of patients with possible secondary immune deficiency states and compared to a control group. Patients with cell-mediated immune deficiency appear to be more prone to warts and zoster infection as compared to patients with humoral immune deficiency. Recurrent herpes simplex infection was not increased in either group."}
{"id": "PMID:1101942", "title": "Ultrasonic measurement of fetal abdomen circumference in the estimation of fetal weight.", "content": "A method of estimating fetal weight by ultrasonic measurement of the fetal abdominal circumference is descirbed. Assessment of birth weight predictions on 140 fetuses who were delivered within 48 hours of this measurement showed that the accuracy of predictions varied with the size of the fetus; at a predicted weight of 1 kg, 95 per cent of birth weights fell within 160 g, while at 2 kg, 3 kg and 4 kg the corresponding values were 290 g, 450 g and 590 g respectively. Expressed as a percentage of the predicted weight, confidence limits remained constant throughout the birth weight range. Extrapolation of these data to routine screening of the obstetric population showed that with a single measurement at 32 weeks menstrual age, 87 per cent of babies below the 5th centile would be detected by this method but that the diagnosis rate would fall to 63 per cent at 38 weeks. The false positive diagnosis rate would remain constant between 32 and 38 weeks at just over 1 per cent.", "contents": "Ultrasonic measurement of fetal abdomen circumference in the estimation of fetal weight. A method of estimating fetal weight by ultrasonic measurement of the fetal abdominal circumference is descirbed. Assessment of birth weight predictions on 140 fetuses who were delivered within 48 hours of this measurement showed that the accuracy of predictions varied with the size of the fetus; at a predicted weight of 1 kg, 95 per cent of birth weights fell within 160 g, while at 2 kg, 3 kg and 4 kg the corresponding values were 290 g, 450 g and 590 g respectively. Expressed as a percentage of the predicted weight, confidence limits remained constant throughout the birth weight range. Extrapolation of these data to routine screening of the obstetric population showed that with a single measurement at 32 weeks menstrual age, 87 per cent of babies below the 5th centile would be detected by this method but that the diagnosis rate would fall to 63 per cent at 38 weeks. The false positive diagnosis rate would remain constant between 32 and 38 weeks at just over 1 per cent."}
{"id": "PMID:1101943", "title": "Comparison of intravenous oxytocin and prostaglandin E2 for induction of labour using automatic and non-automatic infusion techniques.", "content": "A double blind trial of prostaglandin E2 and oxytocin given by intravenous infusion after amniotomy for induction of labour in 100 primigravidae with unfavourable induction features is reported. No clear-cut advantage of either drug emerged although PGE2 was perhaps superior when the cervix was highly unfavourable. Prostaglandin E2 appeared to produce less deleterious effects on the fetus but was associated with a higher incidence of maternal side effects. The automatic Cardiff Infusion apparatus was found to be a safe means of PGE2 infusion and to have advantages over the use of non-automatic techniques both for PGE2 and for oxytocin infusion.", "contents": "Comparison of intravenous oxytocin and prostaglandin E2 for induction of labour using automatic and non-automatic infusion techniques. A double blind trial of prostaglandin E2 and oxytocin given by intravenous infusion after amniotomy for induction of labour in 100 primigravidae with unfavourable induction features is reported. No clear-cut advantage of either drug emerged although PGE2 was perhaps superior when the cervix was highly unfavourable. Prostaglandin E2 appeared to produce less deleterious effects on the fetus but was associated with a higher incidence of maternal side effects. The automatic Cardiff Infusion apparatus was found to be a safe means of PGE2 infusion and to have advantages over the use of non-automatic techniques both for PGE2 and for oxytocin infusion."}
{"id": "PMID:1101944", "title": "The distribution within the placenta, myometrium, and decidua of 24Na-labelled hypertonic saline solution following intra-amniotic or extra-amniotic injection.", "content": "24Na-labelled hypertonic saline solution was injected into the uterus by the intra-amniotic or the extra-amniotic route, and six hours later determinations were made of the 24Na content of the placenta, myometrium, and decidua. The tissue specimens were obtained by subtotal hysterectomy. The activity of 24Na proved to be high in the decidua and in the fetal part of the placenta, but low in the myometrium. During labour, large amounts of prostaglandins have been found in the decidua, but little or none in the placenta. The present findings therefore lend further support to the assumption that hypertonic saline solution provokes abortion by inducing release of lysosomal phospholipase A2 into the cytoplasm of damaged decidual cells, resulting in prostaglandin synthesis and liberation.", "contents": "The distribution within the placenta, myometrium, and decidua of 24Na-labelled hypertonic saline solution following intra-amniotic or extra-amniotic injection. 24Na-labelled hypertonic saline solution was injected into the uterus by the intra-amniotic or the extra-amniotic route, and six hours later determinations were made of the 24Na content of the placenta, myometrium, and decidua. The tissue specimens were obtained by subtotal hysterectomy. The activity of 24Na proved to be high in the decidua and in the fetal part of the placenta, but low in the myometrium. During labour, large amounts of prostaglandins have been found in the decidua, but little or none in the placenta. The present findings therefore lend further support to the assumption that hypertonic saline solution provokes abortion by inducing release of lysosomal phospholipase A2 into the cytoplasm of damaged decidual cells, resulting in prostaglandin synthesis and liberation."}
{"id": "PMID:1101945", "title": "A study of the effect of bromocriptine on serum oestradiol, prolactin, and follicle stimulating hormone levels in puerperal women.", "content": "Nine normal women were examined in the first 11 days of the puerperium. Serum prolactin, oestradiol and follicle simulating hormone (FSH) levels were measured and the FSH response to 100 mug of intravenously administered follicle stimulating hormone/lutenizing hormone-releasing hormone (LH/FSH-RH) was assessed on day 1 post partum in five subjects and on days 4 (two subjects), 6 and 7 post partum in the other four subjects. Bromocriptine therapy was given to six of these women and four to seven days after the start of such therapy, when prolactin levels had fallen to normal non-pregnant levels, these women were retested. Before bromocriptine treatment, the basal FSH levels were unmeasurable in six and low in one other of the nine subjects. After bromocriptine treatment, the basal FSH levels were measurable in four out of six patients and were higher than in the untreated patients (P less than 0-01). The FSH response to LH/FSH-RH was unmeasurable in eight out of nine before treatment, which is less than the response seen in 26 normal men and women (P less than 0-01). There was also no discernible FSH response to LH/FSH-RH after treatment with bromocriptine. Before treatment with bromocriptine, the serum prolactin levels were elevated in all nine women but were within the normal range for non-pregnant women after three days of bromocriptine therapy. Thus postpartum gonadotrophin suppression seemed to depend on high prolactin levels and at least part of the antigonadotrophic action of prolactin seemed to be at pituitary level.", "contents": "A study of the effect of bromocriptine on serum oestradiol, prolactin, and follicle stimulating hormone levels in puerperal women. Nine normal women were examined in the first 11 days of the puerperium. Serum prolactin, oestradiol and follicle simulating hormone (FSH) levels were measured and the FSH response to 100 mug of intravenously administered follicle stimulating hormone/lutenizing hormone-releasing hormone (LH/FSH-RH) was assessed on day 1 post partum in five subjects and on days 4 (two subjects), 6 and 7 post partum in the other four subjects. Bromocriptine therapy was given to six of these women and four to seven days after the start of such therapy, when prolactin levels had fallen to normal non-pregnant levels, these women were retested. Before bromocriptine treatment, the basal FSH levels were unmeasurable in six and low in one other of the nine subjects. After bromocriptine treatment, the basal FSH levels were measurable in four out of six patients and were higher than in the untreated patients (P less than 0-01). The FSH response to LH/FSH-RH was unmeasurable in eight out of nine before treatment, which is less than the response seen in 26 normal men and women (P less than 0-01). There was also no discernible FSH response to LH/FSH-RH after treatment with bromocriptine. Before treatment with bromocriptine, the serum prolactin levels were elevated in all nine women but were within the normal range for non-pregnant women after three days of bromocriptine therapy. Thus postpartum gonadotrophin suppression seemed to depend on high prolactin levels and at least part of the antigonadotrophic action of prolactin seemed to be at pituitary level."}
{"id": "PMID:1101946", "title": "Effect of bromocriptine and chlorotrianisene on inhibition of lactation and serum prolactin. A comparative double-blind study.", "content": "A double-blind study to compare the effect of 2-Br-alpha-ergocryptine (bromocriptine, CB 154, Sandoz) an ergot alkaloid with chlorotrianisene (Tace, Mer-National) on the inhibition of postpartum lactation was carried out in 38 women. At the dosages selected bromocriptine was more effective than chlorotrianisene in inhibiting lactation. Furthermore, bromocriptine significantly reduced serum prolactin levels to low normal values by the seventh day of treatment, whereas chlorotrianisene did not alter the normal progressive reduction in prolactin post-partum. Neither drug had significant toxic effects.", "contents": "Effect of bromocriptine and chlorotrianisene on inhibition of lactation and serum prolactin. A comparative double-blind study. A double-blind study to compare the effect of 2-Br-alpha-ergocryptine (bromocriptine, CB 154, Sandoz) an ergot alkaloid with chlorotrianisene (Tace, Mer-National) on the inhibition of postpartum lactation was carried out in 38 women. At the dosages selected bromocriptine was more effective than chlorotrianisene in inhibiting lactation. Furthermore, bromocriptine significantly reduced serum prolactin levels to low normal values by the seventh day of treatment, whereas chlorotrianisene did not alter the normal progressive reduction in prolactin post-partum. Neither drug had significant toxic effects."}
{"id": "PMID:1101947", "title": "Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine.", "content": "The structure of the unknown modified nucleoside Q, which is present in the first position of the anticodons of Escherichia coli tRNA Tyr, tRNA His, tRNA Asn, tRNA Asp, is proposed to be 7-(4,5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine (1). The structure of Q was deduced by means of its uv absorption, mass spectrometry, proton magnetic resonance spectroscopy, and studies of its chemical reactivity. The structure of Q is unique since it is a derivative of 7-deazaguanosine having cyclopentenediol in the side chain at the C-7 position. This is the first example of purine skeleton modification in a nucleoside from tRNA.", "contents": "Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. The structure of the unknown modified nucleoside Q, which is present in the first position of the anticodons of Escherichia coli tRNA Tyr, tRNA His, tRNA Asn, tRNA Asp, is proposed to be 7-(4,5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine (1). The structure of Q was deduced by means of its uv absorption, mass spectrometry, proton magnetic resonance spectroscopy, and studies of its chemical reactivity. The structure of Q is unique since it is a derivative of 7-deazaguanosine having cyclopentenediol in the side chain at the C-7 position. This is the first example of purine skeleton modification in a nucleoside from tRNA."}
{"id": "PMID:1101948", "title": "Sites of biological methylation of proteins in cultured chick muscle cells.", "content": "The methylation of myosin and other proteins has been studied using primary cultures of 12-day-old embryonic chick leg muscle. The methyl group of [Me-3H] methionine is incorporated into basic amino acid residues with the formation of Nepsilon-monomethyllysine, Nepsilon-dimethyllysine, Nepsilon-trimethyllysine, 3-methylhistidine, NG-monomethylarginine, and NG-dimethylarginine which are isolated from acid hydrolysates of purified myosin, and of proteins from polysomes and from the cytosol of the cultured muscle cells. In the presence of 0.1 mM cycloheximide, incorporation of [Me-3H] methionine into the polysome-bound proteins was decreased to 16.3% of control levels with no change in the pattern of incorporation into the basic amino acid residues, although protein synthesis was inhibited 97.5%. When protein synthesis was allowed to resume in such cultures by the removal of cycloheximide, polypeptides containing labeled N-methylated residues were released from polysomes into the soluble fraction. Polypeptides containing N-methylated amino acids were also released from polysomes following treatment with 2 mM puromycin. Peptidyl-tRNA, isolated from ribosomes after exposure of cultures to [Me-3H] methionine, contained labeled N-methylated amino acids. When [Me-3H] methionine was incorporated in the presence of cycloheximide, the isolated peptidyl-tRNA still contained N-methylated amino acids although the amount of methylation was 22.4% of control levels. These experiments demonstrate that N-methylation of basic amino acid residues in proteins may occur while the polypeptide is still being synthesized on the ribosome. In addition, N-methylation can occur on the nascent polypeptides in the absence of protein synthesis.", "contents": "Sites of biological methylation of proteins in cultured chick muscle cells. The methylation of myosin and other proteins has been studied using primary cultures of 12-day-old embryonic chick leg muscle. The methyl group of [Me-3H] methionine is incorporated into basic amino acid residues with the formation of Nepsilon-monomethyllysine, Nepsilon-dimethyllysine, Nepsilon-trimethyllysine, 3-methylhistidine, NG-monomethylarginine, and NG-dimethylarginine which are isolated from acid hydrolysates of purified myosin, and of proteins from polysomes and from the cytosol of the cultured muscle cells. In the presence of 0.1 mM cycloheximide, incorporation of [Me-3H] methionine into the polysome-bound proteins was decreased to 16.3% of control levels with no change in the pattern of incorporation into the basic amino acid residues, although protein synthesis was inhibited 97.5%. When protein synthesis was allowed to resume in such cultures by the removal of cycloheximide, polypeptides containing labeled N-methylated residues were released from polysomes into the soluble fraction. Polypeptides containing N-methylated amino acids were also released from polysomes following treatment with 2 mM puromycin. Peptidyl-tRNA, isolated from ribosomes after exposure of cultures to [Me-3H] methionine, contained labeled N-methylated amino acids. When [Me-3H] methionine was incorporated in the presence of cycloheximide, the isolated peptidyl-tRNA still contained N-methylated amino acids although the amount of methylation was 22.4% of control levels. These experiments demonstrate that N-methylation of basic amino acid residues in proteins may occur while the polypeptide is still being synthesized on the ribosome. In addition, N-methylation can occur on the nascent polypeptides in the absence of protein synthesis."}
{"id": "PMID:1101949", "title": "Molecular interactions between ribosomal proteins. Evidence for specificity of interaction between isolated proteins.", "content": "The proteins S2, S3, S5, and S10 from the 30S ribosomal subunit of Escherichia coli was studied by analytical ultracentrifugation to characterize them in solution and to determine whether isolated protein-protein interactions exist. Such interactions, if specific, may therefore bear some relationship to the spatial organization of the subunit structure. It was found that protein S2 self-associates to a slight extent and that solution mixtures of S2 and S3 contain only enough dimeric species to account for the S2 dimer. Hence, no observable interaction was detected between S2 and S3. Solution mixtures of proteins S5 and S10 revealed a species of molecular weight greater than either protein. The proposal is that S5 and S10 interact with an association equilibrium constant of 7.6 X 10(-5) M-1 at 3 degrees in a Tris buffer at pH 7.4. It was also shown that solution with a 1:1:1 mixture by mass, of S2, S5, and S10 contained a species possessing a molecular weight consistent with a simple ternary complex of the three proteins.", "contents": "Molecular interactions between ribosomal proteins. Evidence for specificity of interaction between isolated proteins. The proteins S2, S3, S5, and S10 from the 30S ribosomal subunit of Escherichia coli was studied by analytical ultracentrifugation to characterize them in solution and to determine whether isolated protein-protein interactions exist. Such interactions, if specific, may therefore bear some relationship to the spatial organization of the subunit structure. It was found that protein S2 self-associates to a slight extent and that solution mixtures of S2 and S3 contain only enough dimeric species to account for the S2 dimer. Hence, no observable interaction was detected between S2 and S3. Solution mixtures of proteins S5 and S10 revealed a species of molecular weight greater than either protein. The proposal is that S5 and S10 interact with an association equilibrium constant of 7.6 X 10(-5) M-1 at 3 degrees in a Tris buffer at pH 7.4. It was also shown that solution with a 1:1:1 mixture by mass, of S2, S5, and S10 contained a species possessing a molecular weight consistent with a simple ternary complex of the three proteins."}
{"id": "PMID:1101950", "title": "An immunological approach to the role of the low molecular weight subunits in myosin. II. Interaction of myosin and its subfragments with antibodies to the light chains.", "content": "Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic acid) (DTNB) light chain undergoes a precipitation reaction with all of the enzyme species, in which half of the homologous light chain is selectively dissociated. The results suggest that the incomplete dissociation reflects the way in which the light chain is bound, rather than the existence of two distinct species of DTNB 1.c. Little reaction was observed with antisera to alkali-released light chains, indicating that these components in myosin and the subfragments are either largely buried or else conformationally different from the isolated light chains used as immunogens. None of the antisera produced significant changes in Ca2+- or EDTA-ATPase activities. Moreover, calcium regulation through the troponin-tropomyosin system was unaffected by removal of DTNB 1.c. from myosin, as well as from the subfragments. The absolute level of actin-activated ATPase activity was, however, consistently lower in the presence of light chain antisera (or purified IgG and antibody) than in aqueous buffer or nonimmune serum. For both alkali and DTNB 1.c. antisera, this loss in activity seemed to result from steric hindrance of actin binding by antibody bound to undissociated light chain. Experimental conditions which would be expected to weaken such an antigen-antibody interaction, as well as the use of monovalent Fab in place of IgG, decreased the inhibition of activity. Altogether the activity measurements suggest that the light chains, particularly DTNB 1.c., are probably not integral parts of either the hydrolytic or actin-binding sites.", "contents": "An immunological approach to the role of the low molecular weight subunits in myosin. II. Interaction of myosin and its subfragments with antibodies to the light chains. Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic acid) (DTNB) light chain undergoes a precipitation reaction with all of the enzyme species, in which half of the homologous light chain is selectively dissociated. The results suggest that the incomplete dissociation reflects the way in which the light chain is bound, rather than the existence of two distinct species of DTNB 1.c. Little reaction was observed with antisera to alkali-released light chains, indicating that these components in myosin and the subfragments are either largely buried or else conformationally different from the isolated light chains used as immunogens. None of the antisera produced significant changes in Ca2+- or EDTA-ATPase activities. Moreover, calcium regulation through the troponin-tropomyosin system was unaffected by removal of DTNB 1.c. from myosin, as well as from the subfragments. The absolute level of actin-activated ATPase activity was, however, consistently lower in the presence of light chain antisera (or purified IgG and antibody) than in aqueous buffer or nonimmune serum. For both alkali and DTNB 1.c. antisera, this loss in activity seemed to result from steric hindrance of actin binding by antibody bound to undissociated light chain. Experimental conditions which would be expected to weaken such an antigen-antibody interaction, as well as the use of monovalent Fab in place of IgG, decreased the inhibition of activity. Altogether the activity measurements suggest that the light chains, particularly DTNB 1.c., are probably not integral parts of either the hydrolytic or actin-binding sites."}
{"id": "PMID:1101951", "title": "Evidence for photoinduced cross-linkage, in situ, of 30S ribosomal proteins to 16S rRNA.", "content": "The effects of ultraviolet radiation on the 30S ribosomal subunit of Escherichia coli were studied. Irradiation in aqueous solution under anaerobic conditions resulted in a dose-dependent decrease in the separability of the rRNA and protein components of the 30S ribosomal subunit in 4 M urea-3 M LiC1. The results of gel filtration studies of the irradiated ribosomes before and after treatment with pancreatic ribonuclease indicated that the decrease in separability of the ribosome components was a result of the photoinduced formation of covalent RNA-protein cross-links. The number of covalent cross-links was estimated to correspond to less than 3 per 10,000 daltons of ribosomal proteins. One-dimensional gel electrophoresis studies of the course of the photoinduced cross-linkage reaction indicated that cross-linkage of individual 30S ribosomal proteins to the 16S rRNA proceeds in two dose-dependent steps. The first step requires an input of 1 X 10(20) quanta of 253.7 -nm radiation and results in the cross-linkage of at least five ribosomal proteins to the 16S rRNA. The second step requires a total input of 2 X 10(20) quanta of 253.7 -nm radiation, and results in the cross-linkage of most of the remaining 30S ribosomal proteins to the 16S rRNA.", "contents": "Evidence for photoinduced cross-linkage, in situ, of 30S ribosomal proteins to 16S rRNA. The effects of ultraviolet radiation on the 30S ribosomal subunit of Escherichia coli were studied. Irradiation in aqueous solution under anaerobic conditions resulted in a dose-dependent decrease in the separability of the rRNA and protein components of the 30S ribosomal subunit in 4 M urea-3 M LiC1. The results of gel filtration studies of the irradiated ribosomes before and after treatment with pancreatic ribonuclease indicated that the decrease in separability of the ribosome components was a result of the photoinduced formation of covalent RNA-protein cross-links. The number of covalent cross-links was estimated to correspond to less than 3 per 10,000 daltons of ribosomal proteins. One-dimensional gel electrophoresis studies of the course of the photoinduced cross-linkage reaction indicated that cross-linkage of individual 30S ribosomal proteins to the 16S rRNA proceeds in two dose-dependent steps. The first step requires an input of 1 X 10(20) quanta of 253.7 -nm radiation and results in the cross-linkage of at least five ribosomal proteins to the 16S rRNA. The second step requires a total input of 2 X 10(20) quanta of 253.7 -nm radiation, and results in the cross-linkage of most of the remaining 30S ribosomal proteins to the 16S rRNA."}
{"id": "PMID:1101952", "title": "A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.", "content": "An improved method is described for the purification of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate:RNA  nucleotidyltransferase,  EC 2.7.7.6] from Escherichia coli. The method involves lysozyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DNA-cellulose and Bio-Gel A 5m. RNA polymerase is purified to electrophoretic homogeneity in 2 days with a recovery of 45%, resulting in a yield of 250 mg of holoenzyme from 500 g of cells.", "contents": "A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. An improved method is described for the purification of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate:RNA  nucleotidyltransferase,  EC 2.7.7.6] from Escherichia coli. The method involves lysozyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DNA-cellulose and Bio-Gel A 5m. RNA polymerase is purified to electrophoretic homogeneity in 2 days with a recovery of 45%, resulting in a yield of 250 mg of holoenzyme from 500 g of cells."}
{"id": "PMID:1101953", "title": "A fluorometric study of DNA-bound benzo[a]pyrene.", "content": "Comparisons were made among the fluorescence spectra of DNA-bound benzo[a]pyrenes which were produced in vivo and in vitro. DNA from mouse skin treated with benzo[a]pyrene had a maximum emission beyond 400 nm, which was clearly distinguished from that of DNA-bound benzo[a]pyrene 4,5-oxide. The emission spectra from mouse skin were classified into two groups, type I and type II. The former was similar to the spectrum of benzo[a]pyrene, although the two maxima were shifted to longer wavelengths (410 and 435 nm). Type II was characterized by a broad peak around 430 nm. Type I and type II were obtained from different fractions of hydroxylapatite chromatography, but type I was changed into type II during storage. This suggests that type II is a modified product of type I. The emission spectra of both groups also were detected in in vitro activating systems, including photoirradiation, iodine treatment, and hydrogen peroxide treatment. Treatment of Escherichia coli with benzo[a]pyrene during culture produced only fluorescence of type I. Although the relationship between types I and II remains to be established, both types of fluorescence evidently indicate that the conjugated ring structure of the parent compound, benzo[a]pyrene, is preserved intact in DNA-bound benzo[a]pyrene. Several lines of evidence suggest that the proximate (active) form is an unidentified hydroxylated product including an oxy radical, but a cation radical cannot be completely excluded.", "contents": "A fluorometric study of DNA-bound benzo[a]pyrene. Comparisons were made among the fluorescence spectra of DNA-bound benzo[a]pyrenes which were produced in vivo and in vitro. DNA from mouse skin treated with benzo[a]pyrene had a maximum emission beyond 400 nm, which was clearly distinguished from that of DNA-bound benzo[a]pyrene 4,5-oxide. The emission spectra from mouse skin were classified into two groups, type I and type II. The former was similar to the spectrum of benzo[a]pyrene, although the two maxima were shifted to longer wavelengths (410 and 435 nm). Type II was characterized by a broad peak around 430 nm. Type I and type II were obtained from different fractions of hydroxylapatite chromatography, but type I was changed into type II during storage. This suggests that type II is a modified product of type I. The emission spectra of both groups also were detected in in vitro activating systems, including photoirradiation, iodine treatment, and hydrogen peroxide treatment. Treatment of Escherichia coli with benzo[a]pyrene during culture produced only fluorescence of type I. Although the relationship between types I and II remains to be established, both types of fluorescence evidently indicate that the conjugated ring structure of the parent compound, benzo[a]pyrene, is preserved intact in DNA-bound benzo[a]pyrene. Several lines of evidence suggest that the proximate (active) form is an unidentified hydroxylated product including an oxy radical, but a cation radical cannot be completely excluded."}
{"id": "PMID:1101954", "title": "On the mode of action of lomofungin, an inhibitor of RNA synthesis in yeast.", "content": "Lomofungin is a potent inhibitor of RNA synthesis in yeast. Studies on the mode of action of the inhibitor were carried out using yeast RNA polymerases A and B and bacterial RNA polymerase. In vitro inhibition of RNA synthesis is independent of the nature and concentration of the template used and of the nucleoside triphosphate concentration. The extent of inhibition is strongly dependent upon the nature and concentration of divalent cations used to simulate transcription. The three RNA polymerases were inhibited to the same extent in the presence of Mn2+ ions whereas little inhibition was observed with Mg2+ ions. Spectrophotometric studies reveal the formation of different complexes between lomofungin and divalent cations (Mn2+, Mg2+, or Zn2+) with the respective stoichiometries of 0.5, 1, and 2 divalent cations per molecule of lomofungin. The complexes formed depend upon the nature of the divalent cation involved. No direct interaction between lomofungin and DNA could be observed in the presence of divalent cations but evidence is presented that lomofungin interacts with yeast RNA polymerase A. Inhibition of RNA synthesis occurs at the level of both chain initiation and elongation.", "contents": "On the mode of action of lomofungin, an inhibitor of RNA synthesis in yeast. Lomofungin is a potent inhibitor of RNA synthesis in yeast. Studies on the mode of action of the inhibitor were carried out using yeast RNA polymerases A and B and bacterial RNA polymerase. In vitro inhibition of RNA synthesis is independent of the nature and concentration of the template used and of the nucleoside triphosphate concentration. The extent of inhibition is strongly dependent upon the nature and concentration of divalent cations used to simulate transcription. The three RNA polymerases were inhibited to the same extent in the presence of Mn2+ ions whereas little inhibition was observed with Mg2+ ions. Spectrophotometric studies reveal the formation of different complexes between lomofungin and divalent cations (Mn2+, Mg2+, or Zn2+) with the respective stoichiometries of 0.5, 1, and 2 divalent cations per molecule of lomofungin. The complexes formed depend upon the nature of the divalent cation involved. No direct interaction between lomofungin and DNA could be observed in the presence of divalent cations but evidence is presented that lomofungin interacts with yeast RNA polymerase A. Inhibition of RNA synthesis occurs at the level of both chain initiation and elongation."}
{"id": "PMID:1101955", "title": "T7 RNA polymerase: conformation, functional groups, and promotor binding.", "content": "Circular dichroic spectra of T7 RNA polymerase show minima at 222 nm ([theta]m=-7.9 X 10(3) deg cm2/dmol) and 208 nm ([theta]m =-7.55 X 10(3) deg cm2/dmol) and a maximum at 193 nm ([theta]m = 1.2 X 10(4) deg cm2/dmol). The small mean residue ellipticity above 200 nm indicates that the secondary structure contains approximately 12% alpha helix. The secondary structure is unaltered by high salt, glycerol, -SH reagents, nitration of tyrosyl residues, and chelating agents. Binding of the native enzyme to [32P]T7 DNA has been measured by the retention of the protein-[32P]DNA complexes on nitrocellulose filters. At 37degrees T7 RNA polymerase binds to its promoters in the absence of NTP's. Binding and catalytic activity are both abolished at 0degree. Binding of the initiating [gamma-32P]GTP can also be detected by the filter binding assay. Native T7 RNA polymerase is inactivated by reaction with 1 mol of 5,5'-dithiobis(2-nitrobenzoic acid) (Nbs2) or 1 mol of [14C]iodoacetamide. The latter reaction is blocked by Nbs2 suggesting that a single -SH group is required for activity. Alkylation of the -SH group does not alter binding of the enzyme to the DNA template, but modifies the binding of GTP to the enzyme. Nitration of approximately4 surface tyrosyl residues of the protein prevents binding to T7 DNA. The restriction endonuclease, Hpa II, cuts T7 DNA into approximately40 fragments and reduces total RNA synthesis by T7 RNA polymerase by 70%. Fragmentation of the DNA template by Hpa II does not alter the rate of RNA chain initiation by T7 polymerase, and restriction fragments accounting for approximately25% of the T7 DNA still bind tightly to the enzyme. Thus the T7 RNA polymerase promoters remain intact on the restriction fragments. Gel electrophoresis of the transcription products, using restriction fragments as templates, show that of the seven in vitro transcripts produced by T7 RNA polymerase from whole T7 DNA, only the smallest (representing the last 1.5% of the genome) is transcribed from Hpa II fragments. The remaining transcripts are replaced by six new and much shorter mRNA's. The DNA fragments containing the promoters for these mRNA's have been removed from the fragment mix by binding them to the enzyme and retaining the complexes on nitrocellulose filters.", "contents": "T7 RNA polymerase: conformation, functional groups, and promotor binding. Circular dichroic spectra of T7 RNA polymerase show minima at 222 nm ([theta]m=-7.9 X 10(3) deg cm2/dmol) and 208 nm ([theta]m =-7.55 X 10(3) deg cm2/dmol) and a maximum at 193 nm ([theta]m = 1.2 X 10(4) deg cm2/dmol). The small mean residue ellipticity above 200 nm indicates that the secondary structure contains approximately 12% alpha helix. The secondary structure is unaltered by high salt, glycerol, -SH reagents, nitration of tyrosyl residues, and chelating agents. Binding of the native enzyme to [32P]T7 DNA has been measured by the retention of the protein-[32P]DNA complexes on nitrocellulose filters. At 37degrees T7 RNA polymerase binds to its promoters in the absence of NTP's. Binding and catalytic activity are both abolished at 0degree. Binding of the initiating [gamma-32P]GTP can also be detected by the filter binding assay. Native T7 RNA polymerase is inactivated by reaction with 1 mol of 5,5'-dithiobis(2-nitrobenzoic acid) (Nbs2) or 1 mol of [14C]iodoacetamide. The latter reaction is blocked by Nbs2 suggesting that a single -SH group is required for activity. Alkylation of the -SH group does not alter binding of the enzyme to the DNA template, but modifies the binding of GTP to the enzyme. Nitration of approximately4 surface tyrosyl residues of the protein prevents binding to T7 DNA. The restriction endonuclease, Hpa II, cuts T7 DNA into approximately40 fragments and reduces total RNA synthesis by T7 RNA polymerase by 70%. Fragmentation of the DNA template by Hpa II does not alter the rate of RNA chain initiation by T7 polymerase, and restriction fragments accounting for approximately25% of the T7 DNA still bind tightly to the enzyme. Thus the T7 RNA polymerase promoters remain intact on the restriction fragments. Gel electrophoresis of the transcription products, using restriction fragments as templates, show that of the seven in vitro transcripts produced by T7 RNA polymerase from whole T7 DNA, only the smallest (representing the last 1.5% of the genome) is transcribed from Hpa II fragments. The remaining transcripts are replaced by six new and much shorter mRNA's. The DNA fragments containing the promoters for these mRNA's have been removed from the fragment mix by binding them to the enzyme and retaining the complexes on nitrocellulose filters."}
{"id": "PMID:1101956", "title": "Transcription of fractionated calf thymus chromatin by RNA polymerase of calf thymus and Escherichia coli.", "content": "Calf thymus chromatin has been sheared and fractionated on sucrose gradients. Approximately 5-10% of the chromatin is resolved from the bulk of the imput chromatin as a slowly sedimenting fraction. The protein/DNA ratio of the slowly sedimenting fraction is not greatly different from the protein/DNA ratio of the more rapidly sedimenting chromatin fraction. Analysis of DNA of the chromatin fractions by CsC1 equilibrium density gradient centrifugation indicates that DNA of the slowly sedimenting fraction is depleted in the satellite DNA banding at 1.716 g/cm3. The template properties of the chromatin fractions have been examined with Escherichia coli RNA polymerase and form II and form III RNA polymerases of calf thymus. At rate-limiting concentrations, the slowly sedimenting fraction is twofold more active than the rapidly sedimenting fraction as a template for E. coli RNA polymerase. Homologous form II and form III RNA polymerases are respectively 30-fold and 16-fold more active with the slowly sedimenting fraction than the rapidly sedimenting fraction. The activity of form II RNA polymerase in transcribing the slowly sedimenting fraction exceeds its activity in transcribing an equal concentration of native DNA. Kinetic studies, in which RNA polymerase activity is assayed at various concentrations of chromatin, indicate that the greater activity of E. coli RNA polymerase with the slowly sedimenting fraction is due to an increased rate of transcription at saturating concentrations of template (Vmax), and is not due to a lower concentration required for half-maximal rate of transcription (Km). In contrast, the increased rates of transcription of the slowly sedimenting chromatin faction by the homologous polymerases are due to a decrease in concentration required for half-maximal rate of transcription rather than an increased rate of transscription at saturation concentrations of template. The relative degrease of satellite DNA in the slowly sedimenting fraction of chromatin and the enhanced template activity of the slowly sedimenting fraction suggest that this fraction is equivalent to nuclear euchromatin while the more rapidly sedimenting chromatin is equivalent to nuclear heterochromatin.", "contents": "Transcription of fractionated calf thymus chromatin by RNA polymerase of calf thymus and Escherichia coli. Calf thymus chromatin has been sheared and fractionated on sucrose gradients. Approximately 5-10% of the chromatin is resolved from the bulk of the imput chromatin as a slowly sedimenting fraction. The protein/DNA ratio of the slowly sedimenting fraction is not greatly different from the protein/DNA ratio of the more rapidly sedimenting chromatin fraction. Analysis of DNA of the chromatin fractions by CsC1 equilibrium density gradient centrifugation indicates that DNA of the slowly sedimenting fraction is depleted in the satellite DNA banding at 1.716 g/cm3. The template properties of the chromatin fractions have been examined with Escherichia coli RNA polymerase and form II and form III RNA polymerases of calf thymus. At rate-limiting concentrations, the slowly sedimenting fraction is twofold more active than the rapidly sedimenting fraction as a template for E. coli RNA polymerase. Homologous form II and form III RNA polymerases are respectively 30-fold and 16-fold more active with the slowly sedimenting fraction than the rapidly sedimenting fraction. The activity of form II RNA polymerase in transcribing the slowly sedimenting fraction exceeds its activity in transcribing an equal concentration of native DNA. Kinetic studies, in which RNA polymerase activity is assayed at various concentrations of chromatin, indicate that the greater activity of E. coli RNA polymerase with the slowly sedimenting fraction is due to an increased rate of transcription at saturating concentrations of template (Vmax), and is not due to a lower concentration required for half-maximal rate of transcription (Km). In contrast, the increased rates of transcription of the slowly sedimenting chromatin faction by the homologous polymerases are due to a decrease in concentration required for half-maximal rate of transcription rather than an increased rate of transscription at saturation concentrations of template. The relative degrease of satellite DNA in the slowly sedimenting fraction of chromatin and the enhanced template activity of the slowly sedimenting fraction suggest that this fraction is equivalent to nuclear euchromatin while the more rapidly sedimenting chromatin is equivalent to nuclear heterochromatin."}
{"id": "PMID:1101957", "title": "L-phenylalanine:tRNA ligase of Escherichia coli K10. A rapid kinetic investigation of the catalytic reaction.", "content": "The kinetics of the amino acid activation and the transfer of the amino acid to tRNA have been investigated for L-phenylalanine:tRNA ligase of Escherichia coli K10 by stopped-flow and radioactive techniques. The rapid kinetics were followed by the observation of the displacement of the fluorescent dye, 6-p-toluidinylnaphthalene-2-sulfonate from the binding site of L-phenylalanine under conditions where a single active site of the enzyme was involved. The following results are of particular interest. (1) Equilibrium binding of L-phenylalanine and tRNAPhe indicates in each case two sites of interaction with an approximately tenfold difference of the binding affinity. (2) Experimental conditions of the kinetic investigation were chosen to favor reactions at the high affinity binding sites. Under those conditions, the rate constants have been evaluated at 1 mM magnesium to be in the range 12-25 sec-1 for the activation reaction and 42-77 sec-1 for the reverse, the variation of the values depending on those of the dissociation constants used for computation. The rate constant for the transfer reaction is 0.05 sec-1 and for the reverse 0.19 sec-1. The forward reaction is rate limiting for the overall reaction at single turnover and steady-state conditions. (3) All rate constants depend on the concentration of magnesium. Evidence is provided that the transfer occurs via a productive enzyme-tRNAPhe complex which is in a magnesium-dependent equilibrium with an unproductive complex, high magnesium favoring the former. The position of the tRNA-CCA end in the productive complex is such, that the fluorescent dye can be displaced by Phe-tRNAPhe. The thermodynamics of the overall reaction have been treated on the basis of the partial reactions. The free enthalpy of the completed reaction was calculated to be very close to zero. The significance of the adenylate intermediate is discussed with respect to the product inhibition expected on the basis of the tendency of tRNAPhe and L-phenylalanine to form tight complexes with the enzyme.", "contents": "L-phenylalanine:tRNA ligase of Escherichia coli K10. A rapid kinetic investigation of the catalytic reaction. The kinetics of the amino acid activation and the transfer of the amino acid to tRNA have been investigated for L-phenylalanine:tRNA ligase of Escherichia coli K10 by stopped-flow and radioactive techniques. The rapid kinetics were followed by the observation of the displacement of the fluorescent dye, 6-p-toluidinylnaphthalene-2-sulfonate from the binding site of L-phenylalanine under conditions where a single active site of the enzyme was involved. The following results are of particular interest. (1) Equilibrium binding of L-phenylalanine and tRNAPhe indicates in each case two sites of interaction with an approximately tenfold difference of the binding affinity. (2) Experimental conditions of the kinetic investigation were chosen to favor reactions at the high affinity binding sites. Under those conditions, the rate constants have been evaluated at 1 mM magnesium to be in the range 12-25 sec-1 for the activation reaction and 42-77 sec-1 for the reverse, the variation of the values depending on those of the dissociation constants used for computation. The rate constant for the transfer reaction is 0.05 sec-1 and for the reverse 0.19 sec-1. The forward reaction is rate limiting for the overall reaction at single turnover and steady-state conditions. (3) All rate constants depend on the concentration of magnesium. Evidence is provided that the transfer occurs via a productive enzyme-tRNAPhe complex which is in a magnesium-dependent equilibrium with an unproductive complex, high magnesium favoring the former. The position of the tRNA-CCA end in the productive complex is such, that the fluorescent dye can be displaced by Phe-tRNAPhe. The thermodynamics of the overall reaction have been treated on the basis of the partial reactions. The free enthalpy of the completed reaction was calculated to be very close to zero. The significance of the adenylate intermediate is discussed with respect to the product inhibition expected on the basis of the tendency of tRNAPhe and L-phenylalanine to form tight complexes with the enzyme."}
{"id": "PMID:1101958", "title": "Polynucleotide analogs: acrylic acid and maleic acid copolymers of 1-vinyluracil and 9-vinyladenine.", "content": "Radical-induced copolymerization of 1-vinyluracil and maleic anhydride gave, after hydrolysis, a polymer containing a 1:1 monomer ratio of 1-vinyluracil-maleic acid. Gamma-Ray-induced copolymerization of 1-vinyluracil with acrylic acid gave a polymer with a ratio of 1:1.7. Similar treatment of 9-vinlyadenine and acrylic acid resulted in a polymer with a 1:3.2 ratio. These three compounds are potent stimulants of poly (uridylic acid) coded polyphenylalanine synthesis in an in vitro cell free system purified from Escherichia coli MRE 600. The double-stranded polymer, poly(inosinic acid)-poly(cytidylic acid), also stimulates polyphenylalanine synthesis in this assay.", "contents": "Polynucleotide analogs: acrylic acid and maleic acid copolymers of 1-vinyluracil and 9-vinyladenine. Radical-induced copolymerization of 1-vinyluracil and maleic anhydride gave, after hydrolysis, a polymer containing a 1:1 monomer ratio of 1-vinyluracil-maleic acid. Gamma-Ray-induced copolymerization of 1-vinyluracil with acrylic acid gave a polymer with a ratio of 1:1.7. Similar treatment of 9-vinlyadenine and acrylic acid resulted in a polymer with a 1:3.2 ratio. These three compounds are potent stimulants of poly (uridylic acid) coded polyphenylalanine synthesis in an in vitro cell free system purified from Escherichia coli MRE 600. The double-stranded polymer, poly(inosinic acid)-poly(cytidylic acid), also stimulates polyphenylalanine synthesis in this assay."}
{"id": "PMID:1101959", "title": "Inactivation of rat liver RNA polymerases I and II and yeast RNA polymerase I by pyrodixal 5'-phosphate. Evidence for the participation of lysyl residues at the active site.", "content": "Purified DNA-dependent  RNA polymerase forms I (A) and II (B) from rat liver and form I from yeast are rapidly inactivated by pyridoxal 5'-phosphate at pH 8.0. The inhibition is relatively specific since pyridoxamine 5'-phosphate is not an inhibitor and pyridoxal is about 12 times less effective than pyridoxal 5'-phosphate. The inactivation is reversed by high concentrations of amines, and can be made irreversible by reduction with NaBH4. Spectral analysis of the inhibited enzyme and its NaBH4 reduction product indicates that a Schiff base forms between the aldehyde group of pyridoxal 5'-phosphate and one or more amino groups of the protein. Nepsilon-Pyridoxyllysine was identified as the only product in acid hydrolysates of the reduced yeast RNA polymerase I-pyridoxal 5'-phosphate complex. Complete inactivation of yeast polymerase I results in the incorporation of 3-4 mol of pyridoxal 5'-phosphate/1 mol of enzyme. DNA and nucleotide substrates partially protect the enzymes from inactivation. These results suggest that one or more lysyl amino groups are critical for the activity of animal RNA polymerases and show that pyridoxal 5'-phosphate is a suitable probe for studying the active sites of these enzymes. Comparison of the present results with those previously obtained with Eschericha coli RNA polymerase in this laboratory suggest a new degree of structural homology between eucaryotic and procaryotic RNA polymerases.", "contents": "Inactivation of rat liver RNA polymerases I and II and yeast RNA polymerase I by pyrodixal 5'-phosphate. Evidence for the participation of lysyl residues at the active site. Purified DNA-dependent  RNA polymerase forms I (A) and II (B) from rat liver and form I from yeast are rapidly inactivated by pyridoxal 5'-phosphate at pH 8.0. The inhibition is relatively specific since pyridoxamine 5'-phosphate is not an inhibitor and pyridoxal is about 12 times less effective than pyridoxal 5'-phosphate. The inactivation is reversed by high concentrations of amines, and can be made irreversible by reduction with NaBH4. Spectral analysis of the inhibited enzyme and its NaBH4 reduction product indicates that a Schiff base forms between the aldehyde group of pyridoxal 5'-phosphate and one or more amino groups of the protein. Nepsilon-Pyridoxyllysine was identified as the only product in acid hydrolysates of the reduced yeast RNA polymerase I-pyridoxal 5'-phosphate complex. Complete inactivation of yeast polymerase I results in the incorporation of 3-4 mol of pyridoxal 5'-phosphate/1 mol of enzyme. DNA and nucleotide substrates partially protect the enzymes from inactivation. These results suggest that one or more lysyl amino groups are critical for the activity of animal RNA polymerases and show that pyridoxal 5'-phosphate is a suitable probe for studying the active sites of these enzymes. Comparison of the present results with those previously obtained with Eschericha coli RNA polymerase in this laboratory suggest a new degree of structural homology between eucaryotic and procaryotic RNA polymerases."}
{"id": "PMID:1101960", "title": "The effect of salt on phospholipid fatty acid composition in Escherichia coli K-12.", "content": "The fatty acid compositions of the three major phospholipids of Escherichia coli K-12; phosphatidylethanolamine, phosphatidylglycerol and cardiolipin; were determined during growth in media differing in NaCl concentration. Significant differences in fatty acid composition of the phospholipids were observed in the stationary phase cultures, but no appreciable differences were found in early exponential cultures.", "contents": "The effect of salt on phospholipid fatty acid composition in Escherichia coli K-12. The fatty acid compositions of the three major phospholipids of Escherichia coli K-12; phosphatidylethanolamine, phosphatidylglycerol and cardiolipin; were determined during growth in media differing in NaCl concentration. Significant differences in fatty acid composition of the phospholipids were observed in the stationary phase cultures, but no appreciable differences were found in early exponential cultures."}
{"id": "PMID:1101961", "title": "Nitrogenases of Klebsiella pneumoniae and Azotobacter chroococum. Complex formation between the component proteins.", "content": "1. Sedimentation velocity analyses of mixtures of highly purified component proteins of Azotobacter chroococcum are consistent with the formation of a tight 1 : 1 complex in the absence of Na2 S2 O4. 1 : 1 complex formation between complementary proteins from A. chroococcum and Klebsiella pneumoniae was also observed. The addition of 5 mM Na2 S2 O4 weakened the interaction between the A. chroococcum proteins and also the interaction between complementary proteins of A. chroococcum and K. pneumoniae. 2. Steady-state kinetic data for acetylene reduction at low protein concentrations have been used to calculate association constants at 30 degrees C for the 1 : 1 protein complexes of nitrogenase proteins from A. chroococcum, K. pneumoniae and mixtures of complementary proteins from both organisms. Values centered around 3 - 10(7) M-1 were obtained. 3. The temperature dependence of the association constant for the complex formed by the K. pneumoniae proteins exhibited a sharp break at 17 degrees C with deltaH = 0 and deltaH = 418 kJ - mol-1 above and below 17 degrees C, respectively. 4. The Arrhenius plot for acetylene reduction by the complex formed by the K. pneumoniae proteins was linear over the range 12-40 degrees C with deltaH = 80 kJ - mol-1.", "contents": "Nitrogenases of Klebsiella pneumoniae and Azotobacter chroococum. Complex formation between the component proteins. 1. Sedimentation velocity analyses of mixtures of highly purified component proteins of Azotobacter chroococcum are consistent with the formation of a tight 1 : 1 complex in the absence of Na2 S2 O4. 1 : 1 complex formation between complementary proteins from A. chroococcum and Klebsiella pneumoniae was also observed. The addition of 5 mM Na2 S2 O4 weakened the interaction between the A. chroococcum proteins and also the interaction between complementary proteins of A. chroococcum and K. pneumoniae. 2. Steady-state kinetic data for acetylene reduction at low protein concentrations have been used to calculate association constants at 30 degrees C for the 1 : 1 protein complexes of nitrogenase proteins from A. chroococcum, K. pneumoniae and mixtures of complementary proteins from both organisms. Values centered around 3 - 10(7) M-1 were obtained. 3. The temperature dependence of the association constant for the complex formed by the K. pneumoniae proteins exhibited a sharp break at 17 degrees C with deltaH = 0 and deltaH = 418 kJ - mol-1 above and below 17 degrees C, respectively. 4. The Arrhenius plot for acetylene reduction by the complex formed by the K. pneumoniae proteins was linear over the range 12-40 degrees C with deltaH = 80 kJ - mol-1."}
{"id": "PMID:1101962", "title": "Effect of L-azetidine 2-carboxylic acid on growth and proline metabolism in Escherichia coli.", "content": "The effects of L-azetidine 2-carboxylic acid on growth and proline metabolism in a proline-requiring auxotroph of Escherichia coli are described. The homologue inhibited growth of the wild type and it, alone, did not substitute effectively for proline as a growth supplement for the mutant. In medium containing 0.05 mM proline, the addition of increasing amounts of homologue progressively inhibited growth of the wild type but stimulated growth of the mutant at homologue: proline ratios of 10 : 1 and 50 : 1. This suggested that the homologue exerted a \"sparing effect\" on proline in the mutant. The incorporation of L-[U-14C]proline and L-[3H]azetidine 2-carboxylic acid into hot trichloroacetic acid-insoluble material in the mutant was measured. Amino acid analysis of the insoluble material from cells incubated with radiolabeled proline alone revealed that proline was partially degraded and metabolized to other amino acids prior to incorporation into protein. The addition of unlabeled homologue to the incubation medium significantly reduced proline catabolism, suggesting that the homologue exerted a sparing effect on proline in this mutant. In medium containing unlabeled proline and radiolabeled L-azetidine 2-carboxylic acid, the homologue was incorporated both intact and partially degraded prior to incorporation into protein. Alanine was the major L-azetidine 2-carboxylic acid catabolite.", "contents": "Effect of L-azetidine 2-carboxylic acid on growth and proline metabolism in Escherichia coli. The effects of L-azetidine 2-carboxylic acid on growth and proline metabolism in a proline-requiring auxotroph of Escherichia coli are described. The homologue inhibited growth of the wild type and it, alone, did not substitute effectively for proline as a growth supplement for the mutant. In medium containing 0.05 mM proline, the addition of increasing amounts of homologue progressively inhibited growth of the wild type but stimulated growth of the mutant at homologue: proline ratios of 10 : 1 and 50 : 1. This suggested that the homologue exerted a \"sparing effect\" on proline in the mutant. The incorporation of L-[U-14C]proline and L-[3H]azetidine 2-carboxylic acid into hot trichloroacetic acid-insoluble material in the mutant was measured. Amino acid analysis of the insoluble material from cells incubated with radiolabeled proline alone revealed that proline was partially degraded and metabolized to other amino acids prior to incorporation into protein. The addition of unlabeled homologue to the incubation medium significantly reduced proline catabolism, suggesting that the homologue exerted a sparing effect on proline in this mutant. In medium containing unlabeled proline and radiolabeled L-azetidine 2-carboxylic acid, the homologue was incorporated both intact and partially degraded prior to incorporation into protein. Alanine was the major L-azetidine 2-carboxylic acid catabolite."}
{"id": "PMID:1101963", "title": "External yeast beta-fructosidase. The role of tryptophyl residues in catalysis.", "content": "1. In native invertase at pH 4.6, 23 out of a total of 34 tryptophyl residues are \"exposed\" to oxidation with N-bromosuccinimide, the other residues being apparently shielded from the oxidant within the molecule. 2. Oxidation of 5-6 tryptophyl residues/molecule with N-bromosuccinimide is proportional to the complete inactivation of the enzyme, and appears to be specific for indole chromophore only. The ligand binding and fluorescence measurements indicate that the oxidation of native enzyme, up to 50% inhibition, apparently does not change the conformation and topography of enzymes surface. 3. Invertase is inhibited by diazonium-1-H-tetrazole. Since tyrosine residues can be excluded by nitration studies as catalytically unimportant, it appears that a mocification of a single histidyl residue/molecule with diazonium-1-H-tetrazole is sufficient to abolish the enzymic activity. However, the absence of inhibition with diethyl pyrocarbonate indicates that the inhibition with diazonium-1-H-tetrazole may be mediated through steric hindrance or other indirect effects. 4. The absence of inhibition with 2,4-dinitrophenylhydrazine, trinitro benzenesulfonic acid and 5,5'-dithiobis-(2-nitrobenzoate) indicates that the carbonyl groups of the carbohydrate moiety, free amino and -SH groups are not essential for activity.", "contents": "External yeast beta-fructosidase. The role of tryptophyl residues in catalysis. 1. In native invertase at pH 4.6, 23 out of a total of 34 tryptophyl residues are \"exposed\" to oxidation with N-bromosuccinimide, the other residues being apparently shielded from the oxidant within the molecule. 2. Oxidation of 5-6 tryptophyl residues/molecule with N-bromosuccinimide is proportional to the complete inactivation of the enzyme, and appears to be specific for indole chromophore only. The ligand binding and fluorescence measurements indicate that the oxidation of native enzyme, up to 50% inhibition, apparently does not change the conformation and topography of enzymes surface. 3. Invertase is inhibited by diazonium-1-H-tetrazole. Since tyrosine residues can be excluded by nitration studies as catalytically unimportant, it appears that a mocification of a single histidyl residue/molecule with diazonium-1-H-tetrazole is sufficient to abolish the enzymic activity. However, the absence of inhibition with diethyl pyrocarbonate indicates that the inhibition with diazonium-1-H-tetrazole may be mediated through steric hindrance or other indirect effects. 4. The absence of inhibition with 2,4-dinitrophenylhydrazine, trinitro benzenesulfonic acid and 5,5'-dithiobis-(2-nitrobenzoate) indicates that the carbonyl groups of the carbohydrate moiety, free amino and -SH groups are not essential for activity."}
{"id": "PMID:1101964", "title": "Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.", "content": "Pyruvate decarboxylase dissociates into sub-units of one half the molecular weight at alkaline pH. At the same conditions the cofactors thiamine pyrophosphate and Mg2+ are released and can be separated from the protein. Thiamine pyrophosphate is an obligatory cofactor for reconstitution to the oligomer [1]. In this study the effect of thiamine pyrophosphate derivatives (thiamine monophosphate, thiamine, and thiazole pyrophosphate) upon the reconstitution procedure was evaluated. The complete association of sub-units to form active oligomer was attained only when thiamine pyrophosphate was present. It is concluded that both the pyrimidine ring and the pyrophosphate group are required for productive co-enzyme binding and it is proposed that this interaction effects a conformational change which promotes protomer aggregation to form the enzymatically active holoenzyme. In addition data are presented which indicate that the monomer unit is 60 000 +/- 3000 daltons and that the N-terminal amino acid is histidine. Since the molecular weight of the active oligomer is 230 000 it is proposed that pyruvate decarboxylase is a tetramer comprised of four identical or nearly identical monomer units.", "contents": "Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure. Pyruvate decarboxylase dissociates into sub-units of one half the molecular weight at alkaline pH. At the same conditions the cofactors thiamine pyrophosphate and Mg2+ are released and can be separated from the protein. Thiamine pyrophosphate is an obligatory cofactor for reconstitution to the oligomer [1]. In this study the effect of thiamine pyrophosphate derivatives (thiamine monophosphate, thiamine, and thiazole pyrophosphate) upon the reconstitution procedure was evaluated. The complete association of sub-units to form active oligomer was attained only when thiamine pyrophosphate was present. It is concluded that both the pyrimidine ring and the pyrophosphate group are required for productive co-enzyme binding and it is proposed that this interaction effects a conformational change which promotes protomer aggregation to form the enzymatically active holoenzyme. In addition data are presented which indicate that the monomer unit is 60 000 +/- 3000 daltons and that the N-terminal amino acid is histidine. Since the molecular weight of the active oligomer is 230 000 it is proposed that pyruvate decarboxylase is a tetramer comprised of four identical or nearly identical monomer units."}
{"id": "PMID:1101965", "title": "Multiple forms of mitochondrial alcohol dehydrogenase in Saccharomyces cerevisiae.", "content": "Three forms of NAD-dependent alcohol dehydrogenase have been characterized in yeast by their heat sensitivity, their specificity and their electrophoretic patterns. Thermal stability increases in the following order: alcohol dehydrogenase I (fermentative enzyme), alcohol dehydrogenase II (oxidative enzyme), alcohol dehydrogenase III (mitochondrial enzyme). Work with isolated mitochondria shows that alcohol dehydrogenase III is the only form of alcohol dehydrogenase present in these organelles. Starch gel electrophoresis of alcohol dehydrogenase III reveals an active zone of slow migration which consists of five sub-bands. The relative activity of these five sub-bands varies with the conditions of growth. Mitochondrial alcohol dehydrogenase represents never more than 10% of the total cellular alcohol dehydrogenase. Information for its biosynthesis seems to be located in nucleic DNA. The mitochondrial enzyme shows a high affinity for alcohols with a double bond conjugated to the alcohol function.", "contents": "Multiple forms of mitochondrial alcohol dehydrogenase in Saccharomyces cerevisiae. Three forms of NAD-dependent alcohol dehydrogenase have been characterized in yeast by their heat sensitivity, their specificity and their electrophoretic patterns. Thermal stability increases in the following order: alcohol dehydrogenase I (fermentative enzyme), alcohol dehydrogenase II (oxidative enzyme), alcohol dehydrogenase III (mitochondrial enzyme). Work with isolated mitochondria shows that alcohol dehydrogenase III is the only form of alcohol dehydrogenase present in these organelles. Starch gel electrophoresis of alcohol dehydrogenase III reveals an active zone of slow migration which consists of five sub-bands. The relative activity of these five sub-bands varies with the conditions of growth. Mitochondrial alcohol dehydrogenase represents never more than 10% of the total cellular alcohol dehydrogenase. Information for its biosynthesis seems to be located in nucleic DNA. The mitochondrial enzyme shows a high affinity for alcohols with a double bond conjugated to the alcohol function."}
{"id": "PMID:1101966", "title": "Synthesis and degradation of early mRNA in lambda phage.", "content": "Using two different Escherichia coli mutants defective in elongation factors EFG and EFTs required for peptide synthesis, lambda phage with or without a tof mutation was analysed for synthesis of early mRNA by DNA-RNA hybridization technique. (1) In CP78G carrying temperature-sensitive elongation factor G, shift-up to high temperature (41 degrees C) in the middle of phage infection did not affect early mRNA synthesis with lambdatof+ phage but did inhibit it with lambdatof- phage. (2) In HAK88 carrying temperature-sensitive elongation factor Ts, shift-up to 41 degrees C dropped total cellular RNA synthesis to below 10-20 percent of the control in the presence or absence of phage infection (stringent control). Under such a condition for suppressed protein synthesis, lambda early mRNA synthesis was completely blocked with both tof+ and tof- phage infection. Thus, stringent control seems to exert its effect on lambda early mRNA synthesis. (3) Addition of chloramphenicol, which is known to relax the stringent control of RNA synthesis, to the culture of phage-infected HAK88 at 41 degrees C resulted in full recovery of tof+-mRNA synthesis, but only in partial recovery to tof --mRNA synthesis. (4) Analysis of the stability of lambda mRNA indicated an exponential decay, and a halflife of tof --mRNA of 6 min when that of tof+-mRNA was 13 min at 37 degrees C.", "contents": "Synthesis and degradation of early mRNA in lambda phage. Using two different Escherichia coli mutants defective in elongation factors EFG and EFTs required for peptide synthesis, lambda phage with or without a tof mutation was analysed for synthesis of early mRNA by DNA-RNA hybridization technique. (1) In CP78G carrying temperature-sensitive elongation factor G, shift-up to high temperature (41 degrees C) in the middle of phage infection did not affect early mRNA synthesis with lambdatof+ phage but did inhibit it with lambdatof- phage. (2) In HAK88 carrying temperature-sensitive elongation factor Ts, shift-up to 41 degrees C dropped total cellular RNA synthesis to below 10-20 percent of the control in the presence or absence of phage infection (stringent control). Under such a condition for suppressed protein synthesis, lambda early mRNA synthesis was completely blocked with both tof+ and tof- phage infection. Thus, stringent control seems to exert its effect on lambda early mRNA synthesis. (3) Addition of chloramphenicol, which is known to relax the stringent control of RNA synthesis, to the culture of phage-infected HAK88 at 41 degrees C resulted in full recovery of tof+-mRNA synthesis, but only in partial recovery to tof --mRNA synthesis. (4) Analysis of the stability of lambda mRNA indicated an exponential decay, and a halflife of tof --mRNA of 6 min when that of tof+-mRNA was 13 min at 37 degrees C."}
{"id": "PMID:1101967", "title": "Aminoacylation of Phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial tRNAsMet and of Escherichia coli tRNAsMet by homologous and heterologous enzymes.", "content": "Met-tRNA synthetase from Paseolus vulgaris cytoplasm could be separated from its chloroplastic or mitochondrial counterpart by DEAE-cellulose chromatography, but the Met-tRNA synthetase from the two latter organelles could not be distinguished using DEAE-cellulose, hydroxyapatite or CM-Sephadex chromatography. As revealed by reverse-phase chromatography, bean cytoplasm contains 2 tRNAsMet; only one is charged by chloroplast, mitochondrial or Escherichia coli Met-tRNA synthetase. Mitochondria contain, in addition to the 2 cytoplasmic tRNAsMet, 3 mitochondria-spedific tRNAsMet; 2 can be formylated by the mitochondrial or the E. coli transformylase; all 3 are charged by mitochondrial, chloroplastic or E, coli Met-tRNA synthetase; none is charged by the cytoplasmic enzyme. Chloroplasts contain, in addition to the 2 cytoplasmic tRNAsMet, 3 chloroplast-specific tRNAsMet, different from the mitochondrial tRNAsMet; one is formylatable by the chloroplastic or the E. coli transformylase; all 3 are charged by chloroplastic, mitochondrial or E. coli Met-tRNA synthetase; only one is charged by the cytoplasmic enzyme. Of the 3 E. coli tRNAsMet, only the formylatable species can be charged by bean cytoplasmic, chloroplastic or mitochondrial Met-tRNA synthetase.", "contents": "Aminoacylation of Phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial tRNAsMet and of Escherichia coli tRNAsMet by homologous and heterologous enzymes. Met-tRNA synthetase from Paseolus vulgaris cytoplasm could be separated from its chloroplastic or mitochondrial counterpart by DEAE-cellulose chromatography, but the Met-tRNA synthetase from the two latter organelles could not be distinguished using DEAE-cellulose, hydroxyapatite or CM-Sephadex chromatography. As revealed by reverse-phase chromatography, bean cytoplasm contains 2 tRNAsMet; only one is charged by chloroplast, mitochondrial or Escherichia coli Met-tRNA synthetase. Mitochondria contain, in addition to the 2 cytoplasmic tRNAsMet, 3 mitochondria-spedific tRNAsMet; 2 can be formylated by the mitochondrial or the E. coli transformylase; all 3 are charged by mitochondrial, chloroplastic or E, coli Met-tRNA synthetase; none is charged by the cytoplasmic enzyme. Chloroplasts contain, in addition to the 2 cytoplasmic tRNAsMet, 3 chloroplast-specific tRNAsMet, different from the mitochondrial tRNAsMet; one is formylatable by the chloroplastic or the E. coli transformylase; all 3 are charged by chloroplastic, mitochondrial or E. coli Met-tRNA synthetase; only one is charged by the cytoplasmic enzyme. Of the 3 E. coli tRNAsMet, only the formylatable species can be charged by bean cytoplasmic, chloroplastic or mitochondrial Met-tRNA synthetase."}
{"id": "PMID:1101968", "title": "Substrate specificity of Salmonella typhimurium RNAase III and the nature of products formed.", "content": "RNAase III, an endonuclease specific for double-stranded substrates, has been obtained in a highly purified form from extracts of Salmonella typhimurium. Poly (I-C) and a mixture of poly(A) and poly(U) (1 : 1), the latter in presence of 5 mM Mg2+, act as excellent substrates. Poly(I-C) is only partially hydrolysed by RNAase III and the product or products are acted upon by both RNAase I and RNAase II indicating that the products may be oligonucleotides. This conclusion is supported by two-dimensional chromatography on DEAE paper.", "contents": "Substrate specificity of Salmonella typhimurium RNAase III and the nature of products formed. RNAase III, an endonuclease specific for double-stranded substrates, has been obtained in a highly purified form from extracts of Salmonella typhimurium. Poly (I-C) and a mixture of poly(A) and poly(U) (1 : 1), the latter in presence of 5 mM Mg2+, act as excellent substrates. Poly(I-C) is only partially hydrolysed by RNAase III and the product or products are acted upon by both RNAase I and RNAase II indicating that the products may be oligonucleotides. This conclusion is supported by two-dimensional chromatography on DEAE paper."}
{"id": "PMID:1101970", "title": "[Study of the degradation reaction of paranitrosodimethylaniline by a C-nitrosoreductase of yeast].", "content": "We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB \"batch\", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given by NADH and the others are supplied by the buffer. We could observe two steps in the catalytic mechanism. The enthalpy change of the first one is greater than that of the second. Spectrophotometric and polarographic experiments corroborate this results : two enzymatic steps could be demonstrated. The reduction of p-nitroso-N,N-dimethylaniline is characterized by a peak with a half wave potential at -0,31 V (in M Tris-HCl, pH 8,30). While the amplitude of this one decreases, a second wave appears at -1,03 V.", "contents": "[Study of the degradation reaction of paranitrosodimethylaniline by a C-nitrosoreductase of yeast]. We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB \"batch\", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given by NADH and the others are supplied by the buffer. We could observe two steps in the catalytic mechanism. The enthalpy change of the first one is greater than that of the second. Spectrophotometric and polarographic experiments corroborate this results : two enzymatic steps could be demonstrated. The reduction of p-nitroso-N,N-dimethylaniline is characterized by a peak with a half wave potential at -0,31 V (in M Tris-HCl, pH 8,30). While the amplitude of this one decreases, a second wave appears at -1,03 V."}
{"id": "PMID:1101971", "title": "[Analysis of a small quatity of polypeptides by two-dimensional electrophoresis: application to characterization of proteins].", "content": "A new two-dimensional electrophoresis is described. The first electrophoresis is performed in a 10 p. cent gel (1 X 110 mm) in the presence of 7 M urea; the migration in the second dimension proceeds on a 20 p. cent polyacrylamide sheet (160 X 110 X 1 mm) in the presence of sodium dodecyl sulphate. This system resolves most of the polypeptides obtained by treating a very small amount (about 100 mug) of proteins with cyanogene bromide. Macromolecules can thus be quickly and easily characterized.", "contents": "[Analysis of a small quatity of polypeptides by two-dimensional electrophoresis: application to characterization of proteins]. A new two-dimensional electrophoresis is described. The first electrophoresis is performed in a 10 p. cent gel (1 X 110 mm) in the presence of 7 M urea; the migration in the second dimension proceeds on a 20 p. cent polyacrylamide sheet (160 X 110 X 1 mm) in the presence of sodium dodecyl sulphate. This system resolves most of the polypeptides obtained by treating a very small amount (about 100 mug) of proteins with cyanogene bromide. Macromolecules can thus be quickly and easily characterized."}
{"id": "PMID:1101972", "title": "[Quaternary structure of Escherichia coli polynucleotide phosphorylase: chemical characterization of form A].", "content": "We had previously shown the existence of two classes of polynucleotide phosphorylases : Form A which is made of alpha chains and carries the catalytic sites ; and form B which is constituted of alpha chains and of beta chains. We performed some chemical analyses of form A (N-terminal sequence, amino acid composition, peptide mapping) which suggest that the alpha chains are all identical and moreover that they have no relationship with the beta chains. The latter do not therefore derive from a partial proteolytic degradation of the alpha chains and can therefore be considered as true subunits of the enzyme.", "contents": "[Quaternary structure of Escherichia coli polynucleotide phosphorylase: chemical characterization of form A]. We had previously shown the existence of two classes of polynucleotide phosphorylases : Form A which is made of alpha chains and carries the catalytic sites ; and form B which is constituted of alpha chains and of beta chains. We performed some chemical analyses of form A (N-terminal sequence, amino acid composition, peptide mapping) which suggest that the alpha chains are all identical and moreover that they have no relationship with the beta chains. The latter do not therefore derive from a partial proteolytic degradation of the alpha chains and can therefore be considered as true subunits of the enzyme."}
{"id": "PMID:1101973", "title": "Effects of changes in growth rate on the levels of several aminoacyl-tRNA synthetases in yeast.", "content": "When the growth rate of yeast cells is decreased (for instance by transferring the cells from a rich medium to a poor one, or when the cells enter the stationary phase, or when growth is inhibited by cycloheximide, or when valine is removed from the medium supporting growth of a valine-requiring mutant, or when a thermosensitive mutant is shifted to the non-permissive temperature) there is a decrease in the levels of the four aminoacyl-tRNA synthetases tested. Conversely, an increase in the growth rate is accompanied by an increase in the levels of the four enzymes. But when the growth rate is slowed down by decreasing the temperature of the medium, no effect on the levels of the aminoacyl-tRNA synthetases is observed. These results are consistent with the concept of \"metabolic regulation\" proposed by Parker and neidhardt.", "contents": "Effects of changes in growth rate on the levels of several aminoacyl-tRNA synthetases in yeast. When the growth rate of yeast cells is decreased (for instance by transferring the cells from a rich medium to a poor one, or when the cells enter the stationary phase, or when growth is inhibited by cycloheximide, or when valine is removed from the medium supporting growth of a valine-requiring mutant, or when a thermosensitive mutant is shifted to the non-permissive temperature) there is a decrease in the levels of the four aminoacyl-tRNA synthetases tested. Conversely, an increase in the growth rate is accompanied by an increase in the levels of the four enzymes. But when the growth rate is slowed down by decreasing the temperature of the medium, no effect on the levels of the aminoacyl-tRNA synthetases is observed. These results are consistent with the concept of \"metabolic regulation\" proposed by Parker and neidhardt."}
{"id": "PMID:1101975", "title": "Nuclear power for the artificial heart.", "content": "Studies showed that a totally implantable nuclear-powered artificial heart is practicable, and consequently a prototype system development program was initiated in July 1973. Basic testing of the prototype should be completed in 1977, with extensive studies to qualify the device for clinical use running at least into the early 1980s.", "contents": "Nuclear power for the artificial heart. Studies showed that a totally implantable nuclear-powered artificial heart is practicable, and consequently a prototype system development program was initiated in July 1973. Basic testing of the prototype should be completed in 1977, with extensive studies to qualify the device for clinical use running at least into the early 1980s."}
{"id": "PMID:1101974", "title": "Neurophysiological and behavioral organization of premature infants in the first months of life.", "content": "Many explanations for the current findings of limited group differences between premature and term infants at comparable conceptional ages can be imagined that remain buried in the data as currently presented. For example, some prematures may indeed be advanced in many areas of behavioral and neurophysiological development, but this is counterbalanced by prematures who are significantly delayed in these parameters for medical reasons such as hypoxia or metabolic disturbance, while a third portion of the infants has uneven and disorganizing mixtures of delayed and advanced development in selective modalities. Hopefully future research which takes these various possibilities into consideration will give us a better understanding of this complex problem.", "contents": "Neurophysiological and behavioral organization of premature infants in the first months of life. Many explanations for the current findings of limited group differences between premature and term infants at comparable conceptional ages can be imagined that remain buried in the data as currently presented. For example, some prematures may indeed be advanced in many areas of behavioral and neurophysiological development, but this is counterbalanced by prematures who are significantly delayed in these parameters for medical reasons such as hypoxia or metabolic disturbance, while a third portion of the infants has uneven and disorganizing mixtures of delayed and advanced development in selective modalities. Hopefully future research which takes these various possibilities into consideration will give us a better understanding of this complex problem."}
{"id": "PMID:1101978", "title": "Some thoughts on experimental screening.", "content": "Experimental screening of anticancer drugs is discussed from the standpoint of the need for close co-operation between experimentalists and clinicans in developing therapies for human trials. Particular emphasis is given to screening analogues of known active drugs including the approaches in chemical synthesis and tests of experimental tumor activity, as well as the questions that determine clinical interest and capabilities for human trials. In the area of combinations of two or more drugs, the need for experimental systems providing data for rational design of clinical regimens is highlighted. Means for experimental evaluation of the major variables of dose ratio, dose schedule and sequence of drug administration are considered. Finally, the overall problems of drug screening are discussed within the context of combining drugs with other treatment modalities, such as surgery, radiotherapy, and immunotherapy. The complexities of designing better cancer treatment will require close interaction between experimental and clinical studies.", "contents": "Some thoughts on experimental screening. Experimental screening of anticancer drugs is discussed from the standpoint of the need for close co-operation between experimentalists and clinicans in developing therapies for human trials. Particular emphasis is given to screening analogues of known active drugs including the approaches in chemical synthesis and tests of experimental tumor activity, as well as the questions that determine clinical interest and capabilities for human trials. In the area of combinations of two or more drugs, the need for experimental systems providing data for rational design of clinical regimens is highlighted. Means for experimental evaluation of the major variables of dose ratio, dose schedule and sequence of drug administration are considered. Finally, the overall problems of drug screening are discussed within the context of combining drugs with other treatment modalities, such as surgery, radiotherapy, and immunotherapy. The complexities of designing better cancer treatment will require close interaction between experimental and clinical studies."}
{"id": "PMID:1101979", "title": "Cellulase and protein production from mixed cultures of Trichoderma viride and a yeast.", "content": "Fermentations with mixed cultures of the cellulolytic fungus Trichoderma viride and the yeast Saccharomyces cerevisiae or Candida utilis were examined. The fermentations were carried out in an aerated 5 liter fermentor with NaOH treated barley straw as the cellulose source (2-4%). Yeast was inoculated 24-32 hr after the fungus and the growth of the two organisms was followed through the production of CO2 and cell protein. In comparison with fermentations with T. viride alone, the production time for maximum yields of cellulases and cell protein was reduced by several days, depending on the straw concentrations. The protein content of the growth product was 21-22% and the amino acid composition of the product resembled that of T. viride alone.", "contents": "Cellulase and protein production from mixed cultures of Trichoderma viride and a yeast. Fermentations with mixed cultures of the cellulolytic fungus Trichoderma viride and the yeast Saccharomyces cerevisiae or Candida utilis were examined. The fermentations were carried out in an aerated 5 liter fermentor with NaOH treated barley straw as the cellulose source (2-4%). Yeast was inoculated 24-32 hr after the fungus and the growth of the two organisms was followed through the production of CO2 and cell protein. In comparison with fermentations with T. viride alone, the production time for maximum yields of cellulases and cell protein was reduced by several days, depending on the straw concentrations. The protein content of the growth product was 21-22% and the amino acid composition of the product resembled that of T. viride alone."}
{"id": "PMID:1101980", "title": "[Effect of embichin (HN2) and its monofunctional analog (HN1) on chromatin and DNA matrix activity in an in vitro RNA-polymerase system].", "content": "Embichin inhibited the matrix activity of chromatin and DNA in the RNA-polymerase system in vitro much more than its monofunctional analogue. Chromatin possessed a greater sensitivity to the action of embichin in comparison with the deproteinised DNA. However,  with the action of a monofunctional embichin analogue there was a greater reduction of the matrix activity of DNA in comparison with chromatin. The depression mechanism of the matrix activity of chromatin with the action of embichin was apparently associated with the capacity of the latter to form the DNA-protein bonds in the chromatin composition.", "contents": "[Effect of embichin (HN2) and its monofunctional analog (HN1) on chromatin and DNA matrix activity in an in vitro RNA-polymerase system]. Embichin inhibited the matrix activity of chromatin and DNA in the RNA-polymerase system in vitro much more than its monofunctional analogue. Chromatin possessed a greater sensitivity to the action of embichin in comparison with the deproteinised DNA. However,  with the action of a monofunctional embichin analogue there was a greater reduction of the matrix activity of DNA in comparison with chromatin. The depression mechanism of the matrix activity of chromatin with the action of embichin was apparently associated with the capacity of the latter to form the DNA-protein bonds in the chromatin composition."}
{"id": "PMID:1101982", "title": "[Development and clinical approval of a micromodification of the human leukocyte migration inhibition reaction].", "content": "The author suggests a new modification of the human blood leukocyte migration inhibition test. The results of the clinical approval of the test permit it to be recommended for the investigation in dynamics of the cellular immunity and the humoral factors in ptients with different immunopathological state. This modification permits to carry out 10-15 parallel investigations using small quantitites of the blood.", "contents": "[Development and clinical approval of a micromodification of the human leukocyte migration inhibition reaction]. The author suggests a new modification of the human blood leukocyte migration inhibition test. The results of the clinical approval of the test permit it to be recommended for the investigation in dynamics of the cellular immunity and the humoral factors in ptients with different immunopathological state. This modification permits to carry out 10-15 parallel investigations using small quantitites of the blood."}
{"id": "PMID:1101983", "title": "[Study of the enzymatic activity of the blood leukocytes in experimental nephrotoxic nephritis in rats].", "content": "The enzymatic activity of blood leukocytes was studied in rats with Masugi nephritis by means of the quantitative cytochemical method. Nephrotoxin injection during first hours and days did not produce specific changes in the enzymatic activity. Maximum changes were observed on the 8-th day when the elevation of the activity of dehydrogenase lymphocytes and neutrophils acid phosphatase was observed at the same time with most pronounced histological changes in the kidneys, the decrease of the complement titer and the presence of nephrotoxin and autoglobulin in the renal glomeruli. Later the histological changes became less pronounced, and the enzymatic activity decreased. In rats with proteinuria the activity of the neutrophil leukocytes acid phosphatase was higher than in animals without proteinuria.", "contents": "[Study of the enzymatic activity of the blood leukocytes in experimental nephrotoxic nephritis in rats]. The enzymatic activity of blood leukocytes was studied in rats with Masugi nephritis by means of the quantitative cytochemical method. Nephrotoxin injection during first hours and days did not produce specific changes in the enzymatic activity. Maximum changes were observed on the 8-th day when the elevation of the activity of dehydrogenase lymphocytes and neutrophils acid phosphatase was observed at the same time with most pronounced histological changes in the kidneys, the decrease of the complement titer and the presence of nephrotoxin and autoglobulin in the renal glomeruli. Later the histological changes became less pronounced, and the enzymatic activity decreased. In rats with proteinuria the activity of the neutrophil leukocytes acid phosphatase was higher than in animals without proteinuria."}
{"id": "PMID:1101984", "title": "[Use of an immunothermistographic method for detecting humoral antibodies in the allotransplantation of skin and cornea in rabbits].", "content": "Humoral antibodies were investigated by the method of immunothermistography in 43 rabbits after allotransplantation of the skin and cornea. The method of immunothermistography is based on the changes in thermoconduction of the medium as a result of the antigen-antibody reaction. The antibodies were discovered in the blood serum 3 to 5 days after the skin transplantation. The antibody titre gradually increased. Retransplantation of the skin at first decreased the concentration of antibodies, but the antibody titre rose in 8-12 days. In most of the animals the antibodies disappeared in 2-3 months. The same data were obtained in transplantation of the cornea. A correlation between thermistography data and Hougher's reaction and also the dynamics of the complement titre was found. The method of immunothermistography aids in discovery of the humoral tissue antibodies at the early period of the rejection reaction; it is highly sensitive and simple.", "contents": "[Use of an immunothermistographic method for detecting humoral antibodies in the allotransplantation of skin and cornea in rabbits]. Humoral antibodies were investigated by the method of immunothermistography in 43 rabbits after allotransplantation of the skin and cornea. The method of immunothermistography is based on the changes in thermoconduction of the medium as a result of the antigen-antibody reaction. The antibodies were discovered in the blood serum 3 to 5 days after the skin transplantation. The antibody titre gradually increased. Retransplantation of the skin at first decreased the concentration of antibodies, but the antibody titre rose in 8-12 days. In most of the animals the antibodies disappeared in 2-3 months. The same data were obtained in transplantation of the cornea. A correlation between thermistography data and Hougher's reaction and also the dynamics of the complement titre was found. The method of immunothermistography aids in discovery of the humoral tissue antibodies at the early period of the rejection reaction; it is highly sensitive and simple."}
{"id": "PMID:1101985", "title": "[Organ culture of human embryonic lung tissue].", "content": "Results of morphological study of organotypical cultures of the embryonal pulmonary tissue of 22-24 weeks human fetuses showed that for 30 experimental days they passed the period of adaptation, of the optimal growth and differentiation and gradual death. These periods were also characteristic of organic cultures of the embryonal pulmonary tissue of rodents. However, the processes of growth and differentiation in the corresponding human cultures were seen chiefly in the connective, and in rodents - in the epithelial tissue.", "contents": "[Organ culture of human embryonic lung tissue]. Results of morphological study of organotypical cultures of the embryonal pulmonary tissue of 22-24 weeks human fetuses showed that for 30 experimental days they passed the period of adaptation, of the optimal growth and differentiation and gradual death. These periods were also characteristic of organic cultures of the embryonal pulmonary tissue of rodents. However, the processes of growth and differentiation in the corresponding human cultures were seen chiefly in the connective, and in rodents - in the epithelial tissue."}
{"id": "PMID:1102005", "title": "Potato avoidance during pregnancy in women with a previous infant with either anencephaly and/or spina bifida.", "content": "This investigation is a direct attempt to test Renwick's (1972) hypothesis that 95% of anencephaly and spina bifida (ASB) is preventable by the avoidance of potatoes during pregnancy. Although the numbers involved in the study are small, the investigation fails to support the concept that short-term avoidance of potatoes before conception and throughout pregnancy in women who have had a previous ASB infant reduces the recurrence risk. In the potato-free group, of 23 pregnancies which went to term two infants had ASB (8-7%); whereas in the non-potato-free group, of 56 which went to term two infants had ASB (3-6%). The recurrence risk in both groups was about 5%. The incidence of ASB in the groups shows no significant difference (P = 0-58) and in the potato-free group was not reduced by 95% as postulated by Renwick.", "contents": "Potato avoidance during pregnancy in women with a previous infant with either anencephaly and/or spina bifida. This investigation is a direct attempt to test Renwick's (1972) hypothesis that 95% of anencephaly and spina bifida (ASB) is preventable by the avoidance of potatoes during pregnancy. Although the numbers involved in the study are small, the investigation fails to support the concept that short-term avoidance of potatoes before conception and throughout pregnancy in women who have had a previous ASB infant reduces the recurrence risk. In the potato-free group, of 23 pregnancies which went to term two infants had ASB (8-7%); whereas in the non-potato-free group, of 56 which went to term two infants had ASB (3-6%). The recurrence risk in both groups was about 5%. The incidence of ASB in the groups shows no significant difference (P = 0-58) and in the potato-free group was not reduced by 95% as postulated by Renwick."}
{"id": "PMID:1102049", "title": "Recent advances in acoustical microscopy.", "content": "One cannot be involved in research in universities, government establishments and industry for 35 years without becoming aware of a number of misconceptions about pure science, applied science, engineering and technology in the mind of the public and also in the very places where research is carried out. There is confusion about the aims of research and development. There is uncertainty about the proper roles of universities, research establishments and industry. There is the question whether science is intrinsically good or not. The author illuminates some of these issues by relating milestones in the development of microscopy--optical as well as electron--and gives a snapshot picture of the recent work at Stanford University on the acoustic microscope as a felicitous instance of physics applied to the ever-present desire of mankind: to explore the unknown and to understand nature. Experiments with a mechanically scanning type of acoustic microscope have shown that a resolution limited only by diffraction can be attained and using a sonic frequency of 1-2 GHz, objects of less than 1 mum diameter can in principle be resolved. Various biological tissues have been explored and it appears that the acoustical images contain information substantially different from that obtained optically or electron-optically. Images have also been obtained using the harmonic radiation generated within the object and this mode of operation promises to open up yet another dimension of information. When, in due course, the full power, and the limitations, of the acoustic microscope are appreciated, its use may well lead to advances in science and technology comparable to those brought about by the introduction of the electron microscope.", "contents": "Recent advances in acoustical microscopy. One cannot be involved in research in universities, government establishments and industry for 35 years without becoming aware of a number of misconceptions about pure science, applied science, engineering and technology in the mind of the public and also in the very places where research is carried out. There is confusion about the aims of research and development. There is uncertainty about the proper roles of universities, research establishments and industry. There is the question whether science is intrinsically good or not. The author illuminates some of these issues by relating milestones in the development of microscopy--optical as well as electron--and gives a snapshot picture of the recent work at Stanford University on the acoustic microscope as a felicitous instance of physics applied to the ever-present desire of mankind: to explore the unknown and to understand nature. Experiments with a mechanically scanning type of acoustic microscope have shown that a resolution limited only by diffraction can be attained and using a sonic frequency of 1-2 GHz, objects of less than 1 mum diameter can in principle be resolved. Various biological tissues have been explored and it appears that the acoustical images contain information substantially different from that obtained optically or electron-optically. Images have also been obtained using the harmonic radiation generated within the object and this mode of operation promises to open up yet another dimension of information. When, in due course, the full power, and the limitations, of the acoustic microscope are appreciated, its use may well lead to advances in science and technology comparable to those brought about by the introduction of the electron microscope."}
{"id": "PMID:1102052", "title": "The management of urinary fistulae following cadaveric renal transplantation.", "content": "Vesicoureteric, ureteric and calyceal fistulae in cadaver transplants may be successfully treated by early exploration and nephrostomy splintage with silastic tubing for a period of not less than 6 weeks.", "contents": "The management of urinary fistulae following cadaveric renal transplantation. Vesicoureteric, ureteric and calyceal fistulae in cadaver transplants may be successfully treated by early exploration and nephrostomy splintage with silastic tubing for a period of not less than 6 weeks."}
{"id": "PMID:1102054", "title": "The microbial flora of the vagina and its relationship to bacteriuria in diabetic and non-diabetic women.", "content": "The vaginal and urinary flora have been studied in 57 diabetic and 33 non-diabetic patients. In both populations, no correlation was found between urogenital symptoms and colonisation of the vagina. There was no greater prevalence of potentially pathogenic organisms in diabetic patients, even when control was poor. However, although not statistically significant, group B streptococci were isolated more frequently from poorly controlled diabetics. Bacteriuria was not significantly more common in diabetics. Gram-negative bacilli were found in the vagina significantly more often in bacteriuric patients; the prevalence of such organisms being four-fold greater after the menopause.", "contents": "The microbial flora of the vagina and its relationship to bacteriuria in diabetic and non-diabetic women. The vaginal and urinary flora have been studied in 57 diabetic and 33 non-diabetic patients. In both populations, no correlation was found between urogenital symptoms and colonisation of the vagina. There was no greater prevalence of potentially pathogenic organisms in diabetic patients, even when control was poor. However, although not statistically significant, group B streptococci were isolated more frequently from poorly controlled diabetics. Bacteriuria was not significantly more common in diabetics. Gram-negative bacilli were found in the vagina significantly more often in bacteriuric patients; the prevalence of such organisms being four-fold greater after the menopause."}
{"id": "PMID:1102059", "title": "Antibiotic resistance and survival of E coli in the alimentary tract.", "content": "Some antibiotics tend to select for R-factor-carrying Escherichia coli in the human gut, with complex long-term consequences. Some resistant strains disappear rapidly when treatment ends, while others persist for months in the absence of obvious antibiotic selection pressure, and the performance of individual resistant strains seems to depend more on the nature of the strain than on the plasmid carried. R plasmids are relatively rare in those E coli that colonize well in the gut and resistant bacteria therefore tend to disappear when treatment ends, but this situation could change dramatically if R plasmids became prevalent among those strains of E coli that colonize effectively.", "contents": "Antibiotic resistance and survival of E coli in the alimentary tract. Some antibiotics tend to select for R-factor-carrying Escherichia coli in the human gut, with complex long-term consequences. Some resistant strains disappear rapidly when treatment ends, while others persist for months in the absence of obvious antibiotic selection pressure, and the performance of individual resistant strains seems to depend more on the nature of the strain than on the plasmid carried. R plasmids are relatively rare in those E coli that colonize well in the gut and resistant bacteria therefore tend to disappear when treatment ends, but this situation could change dramatically if R plasmids became prevalent among those strains of E coli that colonize effectively."}
{"id": "PMID:1102060", "title": "The forgotten nodule: complications of sacral nodules in rheumatoid arthritis.", "content": "Nodules commonly occur in rheumatoid arthritis and occasionally give rise to complications. The sacral nodule is easily missed and may ulcerate to produce extensive sacral sores which may lead to serious and even fatal complications in patients with rheumatoid arthritis. Seven cases are reported which illustrate some of these features.", "contents": "The forgotten nodule: complications of sacral nodules in rheumatoid arthritis. Nodules commonly occur in rheumatoid arthritis and occasionally give rise to complications. The sacral nodule is easily missed and may ulcerate to produce extensive sacral sores which may lead to serious and even fatal complications in patients with rheumatoid arthritis. Seven cases are reported which illustrate some of these features."}
{"id": "PMID:1102064", "title": "Which elements are excited in electrical stimulation of mammalian central nervous system: a review.", "content": "(1) There are data on the amount of current necessary to stimulate a myelinated fiber or cell body and/or its axon a given distance away from a monopolar electrode over the entire range of practical interest for intracranial stimulation. Data do not exist for other electrode configurations. (2) Currents from a monopolar cathode of more than 8 times threshold may block action potentials in axons. Therefore, only axons lying in a shell around the electrode are stimulated. Elements very close to the electrode may not be stimulated. Close to an electrode small diameter axons may be stimulated and larger ones may not be. (3) Most, and perhaps all, CNS myelinated fibers have chronaxies of 50-100 musec. When gray matter is stimulated, the chronaxie is often 200-700 musec. It is not clear what is being stimulated in this case. Current-duration relations should be determined for many more responses. (4) There are no current-distance or current-duration data for central finely myelinated or unmyelinated fibers. (5) It takes less cathodal current than anodal to stimulate a myelinated fiber passing by a monopolar electrode. When a monopolar electrode is near a cell body, on the opposite side from the axon, often the lowest threshold is anodal, but sometimes cathodal. Stimulation of a neuron near its cell body is not well understood, but in many cases the axon is probably stimulated. (6) Orientation of cell body and axons with respect to current flow is important. For an axon it is the component of the voltage gradient parallel to the fiber that is important. (7) The pia has a significant resistance and capacitance. Gray matter, white matter, and cerebrospinal fluid have different resistivities, which affect patterns of current flow. (8) More is known about stimulation of mammalian CNS than most workers are aware of. Much of what is unknown seems solvable with current methods.", "contents": "Which elements are excited in electrical stimulation of mammalian central nervous system: a review. (1) There are data on the amount of current necessary to stimulate a myelinated fiber or cell body and/or its axon a given distance away from a monopolar electrode over the entire range of practical interest for intracranial stimulation. Data do not exist for other electrode configurations. (2) Currents from a monopolar cathode of more than 8 times threshold may block action potentials in axons. Therefore, only axons lying in a shell around the electrode are stimulated. Elements very close to the electrode may not be stimulated. Close to an electrode small diameter axons may be stimulated and larger ones may not be. (3) Most, and perhaps all, CNS myelinated fibers have chronaxies of 50-100 musec. When gray matter is stimulated, the chronaxie is often 200-700 musec. It is not clear what is being stimulated in this case. Current-duration relations should be determined for many more responses. (4) There are no current-distance or current-duration data for central finely myelinated or unmyelinated fibers. (5) It takes less cathodal current than anodal to stimulate a myelinated fiber passing by a monopolar electrode. When a monopolar electrode is near a cell body, on the opposite side from the axon, often the lowest threshold is anodal, but sometimes cathodal. Stimulation of a neuron near its cell body is not well understood, but in many cases the axon is probably stimulated. (6) Orientation of cell body and axons with respect to current flow is important. For an axon it is the component of the voltage gradient parallel to the fiber that is important. (7) The pia has a significant resistance and capacitance. Gray matter, white matter, and cerebrospinal fluid have different resistivities, which affect patterns of current flow. (8) More is known about stimulation of mammalian CNS than most workers are aware of. Much of what is unknown seems solvable with current methods."}
{"id": "PMID:1102067", "title": "Carboxyl-terminal sequences of procaryotic ribosomal proteins from Escherichia coli, Bacillus stearothermophilus, and Halobacterium cutirubrum.", "content": "The carboxyl-terminal amino acid sequences of two ribosomal proteins, Escherichia coli L12 and E. coli S4, and the proteins believed to be their equivalents from Bacillus stearothermophilus and Halobacterium cutirubrum, were determined. These proteins are known to be required for peptide chain termination (L12) and in ribosome assembly (S4). The carboxyl-terminal sequences obtained suggest that the E. coli and B. stearothermophilus proteins have retained structural homology in this region, whereas the H. cutirubrum proteins have not.", "contents": "Carboxyl-terminal sequences of procaryotic ribosomal proteins from Escherichia coli, Bacillus stearothermophilus, and Halobacterium cutirubrum. The carboxyl-terminal amino acid sequences of two ribosomal proteins, Escherichia coli L12 and E. coli S4, and the proteins believed to be their equivalents from Bacillus stearothermophilus and Halobacterium cutirubrum, were determined. These proteins are known to be required for peptide chain termination (L12) and in ribosome assembly (S4). The carboxyl-terminal sequences obtained suggest that the E. coli and B. stearothermophilus proteins have retained structural homology in this region, whereas the H. cutirubrum proteins have not."}
{"id": "PMID:1102068", "title": "Reconstitution of 48 S particles with subunit association activity from Escherichia coli 50 S ribosomal components.", "content": "Previous studies have shown that 50 S ribosomal proteins bind sequentially to RNA's of the 50 S subunit via temperature-dependent steps (0 leads to 37 leads to 55 degrees C) to form 28, 32, (43) and 48 S particles (Chu, F.K., and Maeba, P.Y. (1973) Can. J. Biochem. 51, 129-139). When three equivalents of 50 S ribosomal proteins are used per equivalent of 23 S RNA, the yield of reconstituted 48 S particles is greatly increased. The addition of 10 mM spermidine increases the rate of reconstitution and abolishes the need for prior incubation at lower temperatures when 48 S particles are formed at 55 degrees C. In the presence of excess 50 S ribosomal proteins and 10 mM spermidine, 48 S particles formed at 50 degrees C are able to associate with 30 S subunits in a stoichiometric manner to form 70 S complexes. These 48 S particles compete effectively with 50 S subunits for 30 S subunits and have an association activity equal to that of 50 S subunits. As compared to the 34 proteins in 50 S subunits, 4 are either absent or present in marginal amounts, 11 in reduced amounts, and 19 in approximately normal amounts in the 48 S particles.", "contents": "Reconstitution of 48 S particles with subunit association activity from Escherichia coli 50 S ribosomal components. Previous studies have shown that 50 S ribosomal proteins bind sequentially to RNA's of the 50 S subunit via temperature-dependent steps (0 leads to 37 leads to 55 degrees C) to form 28, 32, (43) and 48 S particles (Chu, F.K., and Maeba, P.Y. (1973) Can. J. Biochem. 51, 129-139). When three equivalents of 50 S ribosomal proteins are used per equivalent of 23 S RNA, the yield of reconstituted 48 S particles is greatly increased. The addition of 10 mM spermidine increases the rate of reconstitution and abolishes the need for prior incubation at lower temperatures when 48 S particles are formed at 55 degrees C. In the presence of excess 50 S ribosomal proteins and 10 mM spermidine, 48 S particles formed at 50 degrees C are able to associate with 30 S subunits in a stoichiometric manner to form 70 S complexes. These 48 S particles compete effectively with 50 S subunits for 30 S subunits and have an association activity equal to that of 50 S subunits. As compared to the 34 proteins in 50 S subunits, 4 are either absent or present in marginal amounts, 11 in reduced amounts, and 19 in approximately normal amounts in the 48 S particles."}
{"id": "PMID:1102069", "title": "Nuclear demethylation and C-24 alkylation during ergosterol biosynthesis in Saccharomyces cerevisiae.", "content": "The role of 4,4-dimethylzymosterol (3), 4,4-dimethylfecosterol (4) and 31-norlanosterol (5) in the biosynthesis of ergosterol in Saccharomyces cerevisiae has been investigated. The synthesis of 4 and 5 coupled with the availability of 3 facilitated a search for these sterols in commercial yeast sterol concentrates, fresh laboratory grown yeast and fresh brewery grown yeast. Sterol 4 was not detected in any of these mixtures whereas 5 was found in the first and last and 3 was present in all three sources investigated. Investigation of incorporation of [2-3H]lanosterol into 3, 4 and 5 revealed significant incorporation into 3 but neither 4 nor 5. This observation suggests the principle pathway for ergosterol biosynthesis initially involved 1 leads to 3 leads to 7. Incubation of a mixture of [2,4-3H]zymosterol and [26,27-14C]lanosterol with S. cerevisiae revealed that during the initial phases of aerobic growth the major route from 7 to ergosterol involves zymosterol (11) but as 11 accumulates 4 alpha-methyl-24-methylenezymosterol (8) assumes equal importance.", "contents": "Nuclear demethylation and C-24 alkylation during ergosterol biosynthesis in Saccharomyces cerevisiae. The role of 4,4-dimethylzymosterol (3), 4,4-dimethylfecosterol (4) and 31-norlanosterol (5) in the biosynthesis of ergosterol in Saccharomyces cerevisiae has been investigated. The synthesis of 4 and 5 coupled with the availability of 3 facilitated a search for these sterols in commercial yeast sterol concentrates, fresh laboratory grown yeast and fresh brewery grown yeast. Sterol 4 was not detected in any of these mixtures whereas 5 was found in the first and last and 3 was present in all three sources investigated. Investigation of incorporation of [2-3H]lanosterol into 3, 4 and 5 revealed significant incorporation into 3 but neither 4 nor 5. This observation suggests the principle pathway for ergosterol biosynthesis initially involved 1 leads to 3 leads to 7. Incubation of a mixture of [2,4-3H]zymosterol and [26,27-14C]lanosterol with S. cerevisiae revealed that during the initial phases of aerobic growth the major route from 7 to ergosterol involves zymosterol (11) but as 11 accumulates 4 alpha-methyl-24-methylenezymosterol (8) assumes equal importance."}
{"id": "PMID:1102070", "title": "Neurological concepts of pain management in head and neck cancer.", "content": "The physiological basis, historical evolution, and our own practice of stereotactic surgery for the relief of cancer pain are briefly described. Thirty-nine procedures have been performed on 33 patients, 27 for pain due to cancer. Lesions have been made both in the specific neospinothalamic pathway of midbrain and thalamus and in the non-specific reticulothalamic system with an overall five per cent mortality and five per cent morbidity. Initial pain relief was achieved in 90 per cent of patients with relief till death in 74 per cent. Lesions were more successful in releiving pain when the specific pain system was involved but only if the analgesia produced by the lesion \"covered\" the patient's pain. On the other hand, dysesthesia occurred only with lesions of the specific system, especially if they involved the thalamus.", "contents": "Neurological concepts of pain management in head and neck cancer. The physiological basis, historical evolution, and our own practice of stereotactic surgery for the relief of cancer pain are briefly described. Thirty-nine procedures have been performed on 33 patients, 27 for pain due to cancer. Lesions have been made both in the specific neospinothalamic pathway of midbrain and thalamus and in the non-specific reticulothalamic system with an overall five per cent mortality and five per cent morbidity. Initial pain relief was achieved in 90 per cent of patients with relief till death in 74 per cent. Lesions were more successful in releiving pain when the specific pain system was involved but only if the analgesia produced by the lesion \"covered\" the patient's pain. On the other hand, dysesthesia occurred only with lesions of the specific system, especially if they involved the thalamus."}
{"id": "PMID:1102071", "title": "Atrophic rhinitis: Proplast as an implant material in surgical treatment.", "content": "The use of Proplast as an implant material is described in the treatment of atrophic rhinitis. It offers an alterantive implant material to bone graft obtained from the patient. There is no resutling morbidity in relationship to a donor site.", "contents": "Atrophic rhinitis: Proplast as an implant material in surgical treatment. The use of Proplast as an implant material is described in the treatment of atrophic rhinitis. It offers an alterantive implant material to bone graft obtained from the patient. There is no resutling morbidity in relationship to a donor site."}
{"id": "PMID:1102072", "title": "Inhibition of oxidative metabolism in Escherichia coli by d-camphor and restoration of oxidase activity by quinones.", "content": "Oxidative metabolism in whole cells of Escherichia coli strain 82/r was inhibited by d-camphor when glucose, pyruvate, or succinate was used as substrate. Inhibition was not due to lower surface tension in d-camphor-treated cell suspensions nor was it a function of cell permeability. Succinic, lactic, and NADH-oxidase activities were inhibited in alumina powder cell-free extracts (80 mug of protein/ml) by d-camphor (1100 mug/ml). NADH: and succinic: DCPIP oxidoreductase enzymes were unaffected by d-camphor. Menadione (vitamin K3) restored succinic, lactic, and NADH-oxidase activities in d-camphor-inhibited cell-free extracts. Concentrations of menadione used to restore succinic and NADH oxidase activities were not stimulatory in non-camphor-treated extracts. Succinic oxidase activity in d-camphor-inhibited cell-free extracts was also restored by ubiquinone (Q6) but not by vitamin K1. These results are interpreted to indicate that d-camphor may affect quinone function in E. coli.", "contents": "Inhibition of oxidative metabolism in Escherichia coli by d-camphor and restoration of oxidase activity by quinones. Oxidative metabolism in whole cells of Escherichia coli strain 82/r was inhibited by d-camphor when glucose, pyruvate, or succinate was used as substrate. Inhibition was not due to lower surface tension in d-camphor-treated cell suspensions nor was it a function of cell permeability. Succinic, lactic, and NADH-oxidase activities were inhibited in alumina powder cell-free extracts (80 mug of protein/ml) by d-camphor (1100 mug/ml). NADH: and succinic: DCPIP oxidoreductase enzymes were unaffected by d-camphor. Menadione (vitamin K3) restored succinic, lactic, and NADH-oxidase activities in d-camphor-inhibited cell-free extracts. Concentrations of menadione used to restore succinic and NADH oxidase activities were not stimulatory in non-camphor-treated extracts. Succinic oxidase activity in d-camphor-inhibited cell-free extracts was also restored by ubiquinone (Q6) but not by vitamin K1. These results are interpreted to indicate that d-camphor may affect quinone function in E. coli."}
{"id": "PMID:1102073", "title": "The influence of hydrostatic pressure upon biochemical activities of heterotrophic bacteria.", "content": "Heterotrophic terrestrial and marine bacterial protein synthetic rates decrease by about 16% of the 1-atm rate for every 100 atm of pressure increment, within the pressure range of 1 to 500 atm. It is proposed that protein synthesis is nore severely inhibited by pressure than are other anabolic or catabolic reactions.", "contents": "The influence of hydrostatic pressure upon biochemical activities of heterotrophic bacteria. Heterotrophic terrestrial and marine bacterial protein synthetic rates decrease by about 16% of the 1-atm rate for every 100 atm of pressure increment, within the pressure range of 1 to 500 atm. It is proposed that protein synthesis is nore severely inhibited by pressure than are other anabolic or catabolic reactions."}
{"id": "PMID:1102079", "title": "Physiological and psychological effects of alpha and EMG feedback training with college drug users: a pilot study.", "content": "Twenty-four volunteer college students who were regular drug users were randomly allocated to three training groups of equal size: alpha feedback, EMG feedback and a joked control group. The subjects were unaware of which feedback condition they received and were asked to practise at home during a six-month follow-up period in order to achieve a relaxed state similar to that experienced during training. No group was successful in retaining gains made in their alpha levels during each session, but the EMG group significantly reduced their muscular activity during training and retained the improvement during follow-up. The alpha and joked groups did not greatly improve their EMG during training but at follow-up achieved the same levels as the EMG group. There was evidence to suggest that a reduction in drug use among light and medium users was maintained during follow-up. Significant and lasting improvements were made by each group in the duration and quality of their sleep and anxiety levels were reduced.", "contents": "Physiological and psychological effects of alpha and EMG feedback training with college drug users: a pilot study. Twenty-four volunteer college students who were regular drug users were randomly allocated to three training groups of equal size: alpha feedback, EMG feedback and a joked control group. The subjects were unaware of which feedback condition they received and were asked to practise at home during a six-month follow-up period in order to achieve a relaxed state similar to that experienced during training. No group was successful in retaining gains made in their alpha levels during each session, but the EMG group significantly reduced their muscular activity during training and retained the improvement during follow-up. The alpha and joked groups did not greatly improve their EMG during training but at follow-up achieved the same levels as the EMG group. There was evidence to suggest that a reduction in drug use among light and medium users was maintained during follow-up. Significant and lasting improvements were made by each group in the duration and quality of their sleep and anxiety levels were reduced."}
{"id": "PMID:1102081", "title": "D. Campbell Meyers, M.D., L.R.C.P., M.R.C.S. (Eng.) 1863-1927, pioneer of Canadian general hospital psychiatry.", "content": "The paper describes the pioneering contributions of Dr. D. Campbell Meyers (1863--1927) to general hospital psychiatry during the first decade of this century at the Toronto General Hospital where, between 1906 - 1911, he operated the first public general hospital psychiatric unit in Canada. His conflict with Dr. C. K. Clarke who opposed his efforts is documented. To honour Meyers' contributions the Department of Psychiatry, Toronto General Hospital, has been named \"The D. Campbell Meyers Memorial Department of Psychiatry\".", "contents": "D. Campbell Meyers, M.D., L.R.C.P., M.R.C.S. (Eng.) 1863-1927, pioneer of Canadian general hospital psychiatry. The paper describes the pioneering contributions of Dr. D. Campbell Meyers (1863--1927) to general hospital psychiatry during the first decade of this century at the Toronto General Hospital where, between 1906 - 1911, he operated the first public general hospital psychiatric unit in Canada. His conflict with Dr. C. K. Clarke who opposed his efforts is documented. To honour Meyers' contributions the Department of Psychiatry, Toronto General Hospital, has been named \"The D. Campbell Meyers Memorial Department of Psychiatry\"."}
{"id": "PMID:1102082", "title": "Clinical studies of methanol extraction residue fraction of Bacillus Calmette-Gu\u00e9rin as an immunostimulant in patients with advanced cancer.", "content": "Forty patients with advanced gastrointestinal cancer, all previous chemotherapy failures, were treated with a methanol extraction residue of Bacillus Calmette-Gu\u00e9rin (MER) alone, administered intradermally in either weekly or every-4-week schedules. The only significant side effect was local cutaneous reaction ranging from papules through pustules to draining ulcerations. On the weekly schedule these reactions frequently reached a point of clinical intolerability. In spite of the advanced nature of their disease, 29% of the patients had increased reactivity to recall antigens; 57% showed an increased reaction to dinitrochlorobenzene challenge during MER therapy; 38% had significant increases in lymphocyte blastogenesis to phytohemagglutinin mitogen, 48% to concanavalin A, and 43% to pokeweed mitogen. Increases (greater than 25%) in immunoglobulins A, M, and G were also observed in 31, 41, and 28% of patients, respectively. MER therapy was associated with increased thymus-derived (T) and bone marrow-derived (B) cells and an increased ratio of T-cells to B-cells. Increases in those determinants reflecting cellular immunity (skin tests, phytohemagglutinin and concanavalin A blastogenesis, and T-cells) showed a positive correlation with patient survival. Increases in those determinants associated with humoral immunity (pokeweed mitogen blastogenesis, immunoglobulins, and B-cells) had, if anything, a negative survival correlation. In comparing administration schedules, the weekly method produced more frequent increases in dinitrochlorobenzene response, more rapid increases with higher peaks in lymphocyte blastogenesis transformation, and more frequent increases in circulating T- and B-cells. The every-4-week method was associated with significantly greater frequencies of increases in immunoglobulins A and M. Of 36 patients with measurable disease, 3 showed greater than 50% objective responses, 2 showed a 25 to 50% response, and 1 showed a mixed response. MER is a potent simulus to cellular and humoral immunity in the patient with advanced gastrointestinal cancer. This stimulation may occasionally result in a clinically evident antineoplastic effect.", "contents": "Clinical studies of methanol extraction residue fraction of Bacillus Calmette-Gu\u00e9rin as an immunostimulant in patients with advanced cancer. Forty patients with advanced gastrointestinal cancer, all previous chemotherapy failures, were treated with a methanol extraction residue of Bacillus Calmette-Gu\u00e9rin (MER) alone, administered intradermally in either weekly or every-4-week schedules. The only significant side effect was local cutaneous reaction ranging from papules through pustules to draining ulcerations. On the weekly schedule these reactions frequently reached a point of clinical intolerability. In spite of the advanced nature of their disease, 29% of the patients had increased reactivity to recall antigens; 57% showed an increased reaction to dinitrochlorobenzene challenge during MER therapy; 38% had significant increases in lymphocyte blastogenesis to phytohemagglutinin mitogen, 48% to concanavalin A, and 43% to pokeweed mitogen. Increases (greater than 25%) in immunoglobulins A, M, and G were also observed in 31, 41, and 28% of patients, respectively. MER therapy was associated with increased thymus-derived (T) and bone marrow-derived (B) cells and an increased ratio of T-cells to B-cells. Increases in those determinants reflecting cellular immunity (skin tests, phytohemagglutinin and concanavalin A blastogenesis, and T-cells) showed a positive correlation with patient survival. Increases in those determinants associated with humoral immunity (pokeweed mitogen blastogenesis, immunoglobulins, and B-cells) had, if anything, a negative survival correlation. In comparing administration schedules, the weekly method produced more frequent increases in dinitrochlorobenzene response, more rapid increases with higher peaks in lymphocyte blastogenesis transformation, and more frequent increases in circulating T- and B-cells. The every-4-week method was associated with significantly greater frequencies of increases in immunoglobulins A and M. Of 36 patients with measurable disease, 3 showed greater than 50% objective responses, 2 showed a 25 to 50% response, and 1 showed a mixed response. MER is a potent simulus to cellular and humoral immunity in the patient with advanced gastrointestinal cancer. This stimulation may occasionally result in a clinically evident antineoplastic effect."}
{"id": "PMID:1102089", "title": "Studies in pericardial function. I. Cardiovascular effects of assisted ventilation, thoracotomy and pericardiectomy in the anesthetized dog.", "content": "In anesthetized dogs, initially breathing spontaneously, the effects of intermittent positive pressure ventilation (IPPV), thoractomy and pericardiectomy on cardiac function, pericardial-ventricular relationships and cardiopulmonary volumes were evaluated. After IPPV stroke volume and work decreased. After thoracotomy there was no further change in cardiac output or work. Pericardiectomy superimposed at this point had no clear-cut effect. End-diastolic transmural pressures (ventricular minus pericardial) and cardiopulmonary volumes were not altered at any stage of the study. In the ventilated open-chest dog the effects of IPPV are greater than those of thoracotomy, and the extent of the effects is related to the level of IPPV. With moderate IPPV and adequate ventilation, the systemic hypotension, marked decreases in cardiac output and reduction of heart volumes reported by previous investigators can be avoided. The removal of the pericardium does not appear to have any deleterious effect in the normal dog.", "contents": "Studies in pericardial function. I. Cardiovascular effects of assisted ventilation, thoracotomy and pericardiectomy in the anesthetized dog. In anesthetized dogs, initially breathing spontaneously, the effects of intermittent positive pressure ventilation (IPPV), thoractomy and pericardiectomy on cardiac function, pericardial-ventricular relationships and cardiopulmonary volumes were evaluated. After IPPV stroke volume and work decreased. After thoracotomy there was no further change in cardiac output or work. Pericardiectomy superimposed at this point had no clear-cut effect. End-diastolic transmural pressures (ventricular minus pericardial) and cardiopulmonary volumes were not altered at any stage of the study. In the ventilated open-chest dog the effects of IPPV are greater than those of thoracotomy, and the extent of the effects is related to the level of IPPV. With moderate IPPV and adequate ventilation, the systemic hypotension, marked decreases in cardiac output and reduction of heart volumes reported by previous investigators can be avoided. The removal of the pericardium does not appear to have any deleterious effect in the normal dog."}
{"id": "PMID:1102103", "title": "Glucose dependent alterations of mitochondrial ultrastructure and enzyme content in mouse pancreatic islets maintained in tissue culture: a morphometrical and biochemical study.", "content": "Isolated islets of Langerhans from mice were maintained in tissue culture for one week at either a high (28 mM) or a low (3.3 mM) extracellular glucose concentration. Electron microscopic morphometry by means of stereological methods revealed a much greater volume of mitochondria in islet cells cultured at low glucose than in those cultured at high glucose. The former islets also showed a higher activity of the mitochondrial marker enzyme, L-3-hydroxyacyl-CoA-dehyrogenase (E.C.1.1.1.35). These results indicate a true mitochondrial hypertrophy at the low glucose concentration. Although it is known from previous studies that the islet cell  metabolism is diminished after low-glucose culture, the present observations of an increased mitochondrial volume probably do not reflect a degenerative process, but rather adaptive changes towards oxidation of energy yielding substrates other than glucose.", "contents": "Glucose dependent alterations of mitochondrial ultrastructure and enzyme content in mouse pancreatic islets maintained in tissue culture: a morphometrical and biochemical study. Isolated islets of Langerhans from mice were maintained in tissue culture for one week at either a high (28 mM) or a low (3.3 mM) extracellular glucose concentration. Electron microscopic morphometry by means of stereological methods revealed a much greater volume of mitochondria in islet cells cultured at low glucose than in those cultured at high glucose. The former islets also showed a higher activity of the mitochondrial marker enzyme, L-3-hydroxyacyl-CoA-dehyrogenase (E.C.1.1.1.35). These results indicate a true mitochondrial hypertrophy at the low glucose concentration. Although it is known from previous studies that the islet cell  metabolism is diminished after low-glucose culture, the present observations of an increased mitochondrial volume probably do not reflect a degenerative process, but rather adaptive changes towards oxidation of energy yielding substrates other than glucose."}
{"id": "PMID:1102104", "title": "Pancreatic islet cytology of Ictaluridac (Teleostei).", "content": "The endocrine pancreas of the bullhead catfish, Ictalurus nebulosus, and the channel catfish, I. punctatas was studied by light and electron microscopy. In addition to the usual A, B and D cells, a fourth endocrine cell type was consistently observed in the electron microscope. All endocrine cell types were innervated. The vesicles of most of the nerve endings were ultrastructurally different from typical adrenergic and cholinergic vesicles, strongly suggesting the possibility of a third autonomic neurotransmitter serving as a regulator of catfish islet secretion.", "contents": "Pancreatic islet cytology of Ictaluridac (Teleostei). The endocrine pancreas of the bullhead catfish, Ictalurus nebulosus, and the channel catfish, I. punctatas was studied by light and electron microscopy. In addition to the usual A, B and D cells, a fourth endocrine cell type was consistently observed in the electron microscope. All endocrine cell types were innervated. The vesicles of most of the nerve endings were ultrastructurally different from typical adrenergic and cholinergic vesicles, strongly suggesting the possibility of a third autonomic neurotransmitter serving as a regulator of catfish islet secretion."}
{"id": "PMID:1102105", "title": "Time of fixation and the localization of Gomori-positive and neurophysin-containing structures in the rat hypothalamus.", "content": "Rats were decapitated and the complete head stored at 4 degrees C. At various time intervals, up to a maximum of 14 days, the brains and pituitary glands were removed and fixed in formalin-picric acid. Neurosecretory material (NSM), as revealed with crotonaldehyde fuchsin, and neurophysin as demonstrated by means of immunoperoxidase histochemistry were localized in the hypothalamic magnocellular nuclei, median eminence and posterior pituitary gland. NSM and neurophysin were present in the paraventricular (PVN) and supra-optic (SON) nuceli up to periods of 14 days although the cellular morphology was disrupted after about 4 days. After 4 days there was an abundance of positive staining \"droplets\" in both the SON, PVN and along the nerve fibers of the supraoptico-neurohypophysial tract. Pituitary gland NSM and neurophysin were still present 14 days post mortem. The slab gradient polyacrylamide gel electrophoresis pattern of soluble proteins extracted from the posterior pituitary gland from the experimental and normal animals showed almost complete identity confirming the minimal action of proteolytic enzymes at 4 degrees C up to a period of 6 days. It is concluded that as long as the tissue is cooled, rapid fixation of hypothalamic tissue is not essential for the localization of NSM and neurophysin.", "contents": "Time of fixation and the localization of Gomori-positive and neurophysin-containing structures in the rat hypothalamus. Rats were decapitated and the complete head stored at 4 degrees C. At various time intervals, up to a maximum of 14 days, the brains and pituitary glands were removed and fixed in formalin-picric acid. Neurosecretory material (NSM), as revealed with crotonaldehyde fuchsin, and neurophysin as demonstrated by means of immunoperoxidase histochemistry were localized in the hypothalamic magnocellular nuclei, median eminence and posterior pituitary gland. NSM and neurophysin were present in the paraventricular (PVN) and supra-optic (SON) nuceli up to periods of 14 days although the cellular morphology was disrupted after about 4 days. After 4 days there was an abundance of positive staining \"droplets\" in both the SON, PVN and along the nerve fibers of the supraoptico-neurohypophysial tract. Pituitary gland NSM and neurophysin were still present 14 days post mortem. The slab gradient polyacrylamide gel electrophoresis pattern of soluble proteins extracted from the posterior pituitary gland from the experimental and normal animals showed almost complete identity confirming the minimal action of proteolytic enzymes at 4 degrees C up to a period of 6 days. It is concluded that as long as the tissue is cooled, rapid fixation of hypothalamic tissue is not essential for the localization of NSM and neurophysin."}
{"id": "PMID:1102106", "title": "Oxytocinase-immunohistochemical demonstration in the immature and term human placenta.", "content": "Oxytocinase (cystine aminopeptidase) was purified from human retroplacental serum by a combination of fractional precipitation, hydroxylapatite chromatography and gel exlusion chromatography on Sephadex G-200. The purified enzyme possessed a specific activity of 980 mIU/mg using L-cystine-di-p-nitroanilide as substrate. This represented a 3200 fold concentration from the starting material in an overall yield of 12%. Antibodies against oxytocinase were raised in rabbits and the gamma-globulins fraction labelled with fluorescein isothiocyanate prior to its use in the immunofluorescence histochemical localization of the enzyme in human placental tissue. Oxytocinase was confined to the syncytiotrophoblastic cells of normal term, and immature placentas as well as in placentas from patients suffering from severe toxaemia. Specific immunofluorescence was also present in the outer margins of the chorion and to a lesser extent in the amnion.", "contents": "Oxytocinase-immunohistochemical demonstration in the immature and term human placenta. Oxytocinase (cystine aminopeptidase) was purified from human retroplacental serum by a combination of fractional precipitation, hydroxylapatite chromatography and gel exlusion chromatography on Sephadex G-200. The purified enzyme possessed a specific activity of 980 mIU/mg using L-cystine-di-p-nitroanilide as substrate. This represented a 3200 fold concentration from the starting material in an overall yield of 12%. Antibodies against oxytocinase were raised in rabbits and the gamma-globulins fraction labelled with fluorescein isothiocyanate prior to its use in the immunofluorescence histochemical localization of the enzyme in human placental tissue. Oxytocinase was confined to the syncytiotrophoblastic cells of normal term, and immature placentas as well as in placentas from patients suffering from severe toxaemia. Specific immunofluorescence was also present in the outer margins of the chorion and to a lesser extent in the amnion."}
{"id": "PMID:1102108", "title": "Types and distribution of lamellar bodies in first cleavage Xenopus embryos.", "content": "Lamellar bodies in first cleavage Xenopus laevis embryos have been examined with the electron microscope. Based on the arrangement and relative thickness of the lamellae, three types of lamellar bodies have been identified. In the first type, a band of two to ten lamellae (spacing about 3.5 nm) was interwined at random. This type of lamellar body was seen closely associated with the concave or the forming face of the Golgi body and may have been derived from this organelle. The frequent association of these lamellar bodies with the growing cleavage furrow may suggest a transfer of materials from the Golgi body to the furrow through the lamellar bodies or their precursors. In the second type, typical myelin-like figures with a repeating distance of about 10 nm were located close to the furrow tip. In the third type, membrane whorls containing tetrads of lamellae (approximately 16 nm thickness) were seen associated with lipid droplets. If the lamellar bodies observed in this study were formed during fixation then the appearance of three different types after the same fixation procedure would indicate the presence of a similar number of pools of precursor materials which differ in their chemical composition and distribution in the embryo.", "contents": "Types and distribution of lamellar bodies in first cleavage Xenopus embryos. Lamellar bodies in first cleavage Xenopus laevis embryos have been examined with the electron microscope. Based on the arrangement and relative thickness of the lamellae, three types of lamellar bodies have been identified. In the first type, a band of two to ten lamellae (spacing about 3.5 nm) was interwined at random. This type of lamellar body was seen closely associated with the concave or the forming face of the Golgi body and may have been derived from this organelle. The frequent association of these lamellar bodies with the growing cleavage furrow may suggest a transfer of materials from the Golgi body to the furrow through the lamellar bodies or their precursors. In the second type, typical myelin-like figures with a repeating distance of about 10 nm were located close to the furrow tip. In the third type, membrane whorls containing tetrads of lamellae (approximately 16 nm thickness) were seen associated with lipid droplets. If the lamellar bodies observed in this study were formed during fixation then the appearance of three different types after the same fixation procedure would indicate the presence of a similar number of pools of precursor materials which differ in their chemical composition and distribution in the embryo."}
{"id": "PMID:1102107", "title": "Histochemical and electron microscopy investigations on medullary bone.", "content": "Folliculin administration to pigeons stimulates the development of medullary bone in marrow spaces of the femora and other long bones. It is a specialized osseus tissue not devoted to mechanical functions and which is rapidly reabsorbed before egg-shell formation. Medullary bone is formed and reabsorbed in the same way as other types of bone. Consequently, because of its very rapid rate of formation and resorption, it represents an ideal tissue for studying osteoblastic, osteoclastic and osteocytic activity, and the calcification process. Medullary bone is deeply stained by PAS, Alcian blue and colloidal iron and is metachromatic after toluidine blue staining. This shows that its interfibrially ground substance contains relatively high amounts of glycoproteins and acid proteoglycans. Calcification initially occurs in maxtrix vesicles (or calcifying globules) which are very numerous between the collagen fibrils of the osteoid tissue, and successively spreads into the surrounding interfibrillar matrix. Here, the crystals are closely related to thin, filament-like organic structures which seem to be components of ground substance proteoglycans. These findings confirm that in medullary bone, as in other types of calcifying tissue, the inorganic substances is initially laid down within calcifying globules and is successively closely related to organic, non-collagenous, filamentous organic structures (crystal ghosts) which probably represent a framework for calcium salt deposition.", "contents": "Histochemical and electron microscopy investigations on medullary bone. Folliculin administration to pigeons stimulates the development of medullary bone in marrow spaces of the femora and other long bones. It is a specialized osseus tissue not devoted to mechanical functions and which is rapidly reabsorbed before egg-shell formation. Medullary bone is formed and reabsorbed in the same way as other types of bone. Consequently, because of its very rapid rate of formation and resorption, it represents an ideal tissue for studying osteoblastic, osteoclastic and osteocytic activity, and the calcification process. Medullary bone is deeply stained by PAS, Alcian blue and colloidal iron and is metachromatic after toluidine blue staining. This shows that its interfibrially ground substance contains relatively high amounts of glycoproteins and acid proteoglycans. Calcification initially occurs in maxtrix vesicles (or calcifying globules) which are very numerous between the collagen fibrils of the osteoid tissue, and successively spreads into the surrounding interfibrillar matrix. Here, the crystals are closely related to thin, filament-like organic structures which seem to be components of ground substance proteoglycans. These findings confirm that in medullary bone, as in other types of calcifying tissue, the inorganic substances is initially laid down within calcifying globules and is successively closely related to organic, non-collagenous, filamentous organic structures (crystal ghosts) which probably represent a framework for calcium salt deposition."}
{"id": "PMID:1102112", "title": "Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.", "content": "The mouse mitochondrial DNA genome has been cloned in Escherichia coli by linking it to the pSC101 plasmid replicon at cohesive-ended cleavage sites generated by Eco Rl restriction endonuclease. The four possible configurations of chimeric molecules that contain the nucleotide sequences of mitochondrial DNA in their native relationship were distinguished by Hind III restriction endonuclease digestion and electron microscopic heteroduplex analysis. Chimeric molecules utilize the pSC101 replication origin and do not maintain the \"D-loop\" region or the low frequency of ribonucleotides found in native mitochondrial DNA. Hybridization of the RNA synthesized in E. coli minicells carrying the four types of chimeras indicates that transcription occurs predominately on the light strand of the mitochondrial DNA in all cases. This result implies that initiation of RNA synthesis occurs within the mitochondrial DNA segment. Although specific polypeptide synthetis is directed by the mitochondrial DNA segment of each of the chimeras in E. coli minicells, the molecular weight distribution of the polypeptides synthesized consists primarily of low molecular weight species and thus differs from that observed in mitochondria in mouse L cells.", "contents": "Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. The mouse mitochondrial DNA genome has been cloned in Escherichia coli by linking it to the pSC101 plasmid replicon at cohesive-ended cleavage sites generated by Eco Rl restriction endonuclease. The four possible configurations of chimeric molecules that contain the nucleotide sequences of mitochondrial DNA in their native relationship were distinguished by Hind III restriction endonuclease digestion and electron microscopic heteroduplex analysis. Chimeric molecules utilize the pSC101 replication origin and do not maintain the \"D-loop\" region or the low frequency of ribonucleotides found in native mitochondrial DNA. Hybridization of the RNA synthesized in E. coli minicells carrying the four types of chimeras indicates that transcription occurs predominately on the light strand of the mitochondrial DNA in all cases. This result implies that initiation of RNA synthesis occurs within the mitochondrial DNA segment. Although specific polypeptide synthetis is directed by the mitochondrial DNA segment of each of the chimeras in E. coli minicells, the molecular weight distribution of the polypeptides synthesized consists primarily of low molecular weight species and thus differs from that observed in mitochondria in mouse L cells."}
{"id": "PMID:1102142", "title": "Observer agreement in evaluating coronary angiograms.", "content": "The reliability of interpretation of coronary arteriography as a diagnostic tool was investigated in a sub-study of the VA Cooperative Study of Surgical Treatment for Coronary Arterial Occlusive Disease. Twenty-two physicians with varying levels of experience read 13 cine angiograms -- blind -- on two different occasions. Analysis of inter- and intraobserver variability showed that angiographic items about which observers were most inconsistent from one reading to the other had the largest interobserver disagreement as well. They were the distal portions of the left anterior descending and left circumflex arteries. Among the items on which there was most consistent agreement -- namely, the right main coronary artery and presence of ventricular aneurysm -- there was most often agreement between observers as well. When individual readers were evaluated, some observers were far more consistent in their own readings of all the angiographic items than others. This intraobserver agreement in turn correlated fairly well with how often they agreed with the other observers and with how much experience they reported having in reading coronary cineangiograms.", "contents": "Observer agreement in evaluating coronary angiograms. The reliability of interpretation of coronary arteriography as a diagnostic tool was investigated in a sub-study of the VA Cooperative Study of Surgical Treatment for Coronary Arterial Occlusive Disease. Twenty-two physicians with varying levels of experience read 13 cine angiograms -- blind -- on two different occasions. Analysis of inter- and intraobserver variability showed that angiographic items about which observers were most inconsistent from one reading to the other had the largest interobserver disagreement as well. They were the distal portions of the left anterior descending and left circumflex arteries. Among the items on which there was most consistent agreement -- namely, the right main coronary artery and presence of ventricular aneurysm -- there was most often agreement between observers as well. When individual readers were evaluated, some observers were far more consistent in their own readings of all the angiographic items than others. This intraobserver agreement in turn correlated fairly well with how often they agreed with the other observers and with how much experience they reported having in reading coronary cineangiograms."}
{"id": "PMID:1102143", "title": "Profile of high risk in people known to have coronary heart disease: A review.", "content": "A review of the current literature of the high-risk factors associated with cardiac morbidity and mortality in patients with established coronary heart disease is presented. Univariate risk factors include age, comorbidity, characteristics of the index coronary event, electrocardiographic findings including ventricular premature beats and response to exercise tests, angiographic severity of the coronary disease, cigarette smoking, psychosocial status, and the chronometric interval after the index coronary event. Multivariate analysis of risk factor combinations is discussed. Risk reduction requires not only an understanding of the clinical course of a disease process, but also, modification of the amenable factors which contribute to excess risk of death.", "contents": "Profile of high risk in people known to have coronary heart disease: A review. A review of the current literature of the high-risk factors associated with cardiac morbidity and mortality in patients with established coronary heart disease is presented. Univariate risk factors include age, comorbidity, characteristics of the index coronary event, electrocardiographic findings including ventricular premature beats and response to exercise tests, angiographic severity of the coronary disease, cigarette smoking, psychosocial status, and the chronometric interval after the index coronary event. Multivariate analysis of risk factor combinations is discussed. Risk reduction requires not only an understanding of the clinical course of a disease process, but also, modification of the amenable factors which contribute to excess risk of death."}
{"id": "PMID:1102145", "title": "Time, place, event of sudden death.", "content": "Sudden death, known since antiquity, is almost always due to cardiovascular disorder, but not exclusively to coronary artery disease. It is postulated that two processes, action of pre-existing risk factors (enlarged heart, abnormal conduction, extra systoles, ischemia) and new, untoward events (acute infarction or ischemia) culminate in the catastrophic event. Sudden death is apparently most likely during waking hours; sleep is a lesser risk. Which subjects are at greatest risk and which circumstances are most dangerous remain unknown and are important topics for further investigation.", "contents": "Time, place, event of sudden death. Sudden death, known since antiquity, is almost always due to cardiovascular disorder, but not exclusively to coronary artery disease. It is postulated that two processes, action of pre-existing risk factors (enlarged heart, abnormal conduction, extra systoles, ischemia) and new, untoward events (acute infarction or ischemia) culminate in the catastrophic event. Sudden death is apparently most likely during waking hours; sleep is a lesser risk. Which subjects are at greatest risk and which circumstances are most dangerous remain unknown and are important topics for further investigation."}
{"id": "PMID:1102147", "title": "Nasal hyposensitization and hay fever.", "content": "A clinical trial of pre-seasonal hyposensitization with intra-nasal grass pollen extract was carried out in forty-two patients. Results were assessed subjectively, by clinical examination during the pollen season, and by measurements of nasal air flow. Useful relief of symptoms was considered to have occurred in fifteen out of twenty-one patients who had pre-seasonal treatment with pollen extract and in five out of twenty-one patients who had similar treatment with carbol saline as a placebo. Of twelve patients who were symptom free in the pollen season ten had nasal hyposensitization. It is concluded that this simple and safe method of treatment gives results comparable with those of pre-seasonal injections.", "contents": "Nasal hyposensitization and hay fever. A clinical trial of pre-seasonal hyposensitization with intra-nasal grass pollen extract was carried out in forty-two patients. Results were assessed subjectively, by clinical examination during the pollen season, and by measurements of nasal air flow. Useful relief of symptoms was considered to have occurred in fifteen out of twenty-one patients who had pre-seasonal treatment with pollen extract and in five out of twenty-one patients who had similar treatment with carbol saline as a placebo. Of twelve patients who were symptom free in the pollen season ten had nasal hyposensitization. It is concluded that this simple and safe method of treatment gives results comparable with those of pre-seasonal injections."}
{"id": "PMID:1102148", "title": "Intranasal betamethasone valerate in the treatment of seasonal rhinitis.", "content": "Betamethasone valerate aerosol given in doses of 100 mug into each nostril twice daily was compared with a placebo in a double-blind, cross-over trial involving thirty patients with seasonal rhinitis. Patients recorded symptoms of eye irritation and watering, sneezing, rhinorrhoea, and nasal blockage, on a diary card. Analysis of the symptom scores showed that nasal symptoms were significantly better on betamethasone valerate than on placebo (P less than 0.01) and that nasal blockage in particular was improved (P less than 0.001). The patients' preference was significantly in favour of the active compound (p less than 0.02) and no side-effects were noted. It is concluded that betamethasone valerate offers a safe and effective form of treatment for seasonal rhinitis.", "contents": "Intranasal betamethasone valerate in the treatment of seasonal rhinitis. Betamethasone valerate aerosol given in doses of 100 mug into each nostril twice daily was compared with a placebo in a double-blind, cross-over trial involving thirty patients with seasonal rhinitis. Patients recorded symptoms of eye irritation and watering, sneezing, rhinorrhoea, and nasal blockage, on a diary card. Analysis of the symptom scores showed that nasal symptoms were significantly better on betamethasone valerate than on placebo (P less than 0.01) and that nasal blockage in particular was improved (P less than 0.001). The patients' preference was significantly in favour of the active compound (p less than 0.02) and no side-effects were noted. It is concluded that betamethasone valerate offers a safe and effective form of treatment for seasonal rhinitis."}
{"id": "PMID:1102149", "title": "The effect of beclomethasone dipropionate aerosol on allergen induced nasal stenosis.", "content": "Nasal resistance to air flow has been used to evaluate the effect of beclomethasone dipropionate aerosol on allergen induced nasal stenosis. Sixteen patients with allergic rhinitis due to pollen were investigated in a randomized double-blind cross-over study with beclomethasone dipropionate aerosol and placebo aerosol. Only patients reacting to challenge were chosen. The study was carried out in the pollen-free season. After 1 week on either active or placebo aerosol a basic resistance value was determined followed by allergen challenge. Nasal resistance was determined 15 min and 7 hr after challenge. The aerosols were changed and after another week the procedure was repeated. There was significant preference (P less than 0.01) for beclomethasone dipropionate aerosol.", "contents": "The effect of beclomethasone dipropionate aerosol on allergen induced nasal stenosis. Nasal resistance to air flow has been used to evaluate the effect of beclomethasone dipropionate aerosol on allergen induced nasal stenosis. Sixteen patients with allergic rhinitis due to pollen were investigated in a randomized double-blind cross-over study with beclomethasone dipropionate aerosol and placebo aerosol. Only patients reacting to challenge were chosen. The study was carried out in the pollen-free season. After 1 week on either active or placebo aerosol a basic resistance value was determined followed by allergen challenge. Nasal resistance was determined 15 min and 7 hr after challenge. The aerosols were changed and after another week the procedure was repeated. There was significant preference (P less than 0.01) for beclomethasone dipropionate aerosol."}
{"id": "PMID:1102152", "title": "Application of pepsin-digested horse antibodies for quantitative immunoelectrophoresis.", "content": "The majority of the precipitating antibodies in hyperimmune horse serum belong to the beta globulins, as demonstrated by reversed immunoelectrophoresis. As these antibodies migrate in agarose gel during electrophoresis in conventional pH = 8.6-8.9 buffers, horse antiserum cannot be used satisfactorily for quantitative immunoelectrophoresis. On pepsin digestion of horse antiserum F(ab)' 2 fragments are generated which migrate with the gamma globulins. This cleaved product works well in quantitative immunoelectrophoretic techniques.", "contents": "Application of pepsin-digested horse antibodies for quantitative immunoelectrophoresis. The majority of the precipitating antibodies in hyperimmune horse serum belong to the beta globulins, as demonstrated by reversed immunoelectrophoresis. As these antibodies migrate in agarose gel during electrophoresis in conventional pH = 8.6-8.9 buffers, horse antiserum cannot be used satisfactorily for quantitative immunoelectrophoresis. On pepsin digestion of horse antiserum F(ab)' 2 fragments are generated which migrate with the gamma globulins. This cleaved product works well in quantitative immunoelectrophoretic techniques."}
{"id": "PMID:1102159", "title": "Influence of adrenaline on the dissemination of antibody-producing cells from the spleen.", "content": "Plaque-forming cells (PFC) and rosette-forming cells (RFC) were quantificated in splenic venous and splenic arterial blood and in spleen suspensions of guinea-pigs during a secondary immune response to sheep red blood cells (SRBC). The splenic veno-arterial differences in content of PFC and RFC were determined, indicating whether there had been a release of such cells from the spleen into the blood. The effect of an intracardial injection of adrenaline on the release of immune lymphocytes was investigated. In immunized control animals a splenic release of antigen-binding and antibody-forming cells was found, the release being restricted to the peak of the immune response in the spleen. However, after exogenous adrenaline a considerably increased release of both antigen-binding and antibody-forming cells occurred during a longer period of the immune response. Thus, adrenaline caused an enormous release of PFC from the spleen into the blood on day 4 of the secondary immune response, resulting in a diminished number of PFC remaining in the spleen after the treatment. A physiological significance of an adrenaline-induced dissemination of immune lymphocytes in the body during an immune response to a severe infectious disease is suggested.", "contents": "Influence of adrenaline on the dissemination of antibody-producing cells from the spleen. Plaque-forming cells (PFC) and rosette-forming cells (RFC) were quantificated in splenic venous and splenic arterial blood and in spleen suspensions of guinea-pigs during a secondary immune response to sheep red blood cells (SRBC). The splenic veno-arterial differences in content of PFC and RFC were determined, indicating whether there had been a release of such cells from the spleen into the blood. The effect of an intracardial injection of adrenaline on the release of immune lymphocytes was investigated. In immunized control animals a splenic release of antigen-binding and antibody-forming cells was found, the release being restricted to the peak of the immune response in the spleen. However, after exogenous adrenaline a considerably increased release of both antigen-binding and antibody-forming cells occurred during a longer period of the immune response. Thus, adrenaline caused an enormous release of PFC from the spleen into the blood on day 4 of the secondary immune response, resulting in a diminished number of PFC remaining in the spleen after the treatment. A physiological significance of an adrenaline-induced dissemination of immune lymphocytes in the body during an immune response to a severe infectious disease is suggested."}
{"id": "PMID:1102160", "title": "Nippostrongylus brasiliensis in young rats. Lymphocytes expel larval infections but not adult worms.", "content": "Young rats were not able to expel adult N. brasiliensis infections even when the worms were damaged by antibodies and the young rats were given all the cellular components (sensitized lymphocytes and bone marrow cells) shown to be necessary for the expulsion of antibody-damaged worms from adult rats. In contrast, most of the worms were expelled from young rats given sensitized lymph node cells on the day of a larval infection. These results show that the reduced ability of young rats to respond to infection by producing sensitized lymphocytes only partly explains their inability to expel the worms. It was not possible to explain the failure of young rats to expel adult worms by hypothesizing that they develop an active factor which prevents the cells from acting on the worms. It is also unlikely that worms persist in young rats because they differ in their susceptibility to cells compared with antibody-damaged worms from mature rats. This work suggests that the immune mechanism which affects the immature stages of this nematode may differ from that which controls the adult stages.", "contents": "Nippostrongylus brasiliensis in young rats. Lymphocytes expel larval infections but not adult worms. Young rats were not able to expel adult N. brasiliensis infections even when the worms were damaged by antibodies and the young rats were given all the cellular components (sensitized lymphocytes and bone marrow cells) shown to be necessary for the expulsion of antibody-damaged worms from adult rats. In contrast, most of the worms were expelled from young rats given sensitized lymph node cells on the day of a larval infection. These results show that the reduced ability of young rats to respond to infection by producing sensitized lymphocytes only partly explains their inability to expel the worms. It was not possible to explain the failure of young rats to expel adult worms by hypothesizing that they develop an active factor which prevents the cells from acting on the worms. It is also unlikely that worms persist in young rats because they differ in their susceptibility to cells compared with antibody-damaged worms from mature rats. This work suggests that the immune mechanism which affects the immature stages of this nematode may differ from that which controls the adult stages."}
{"id": "PMID:1102161", "title": "The effect of human alpha 2-macroglobulin on the restoration of humoral responsiveness in x-irradiated mice.", "content": "The effect of human alpha 2-macroglobulin on the recovery of the humoral immune response in X-irradiated mice has been assessed by measurement of antibody-forming cells in the spleen 5 days after challenge with sheep erythrocytes. The capacity of this protein to promote the recovery of immune responsiveness was variable, and appeared to be influenced by the strain and sex of the mice, and the dose of alpha2M given.", "contents": "The effect of human alpha 2-macroglobulin on the restoration of humoral responsiveness in x-irradiated mice. The effect of human alpha 2-macroglobulin on the recovery of the humoral immune response in X-irradiated mice has been assessed by measurement of antibody-forming cells in the spleen 5 days after challenge with sheep erythrocytes. The capacity of this protein to promote the recovery of immune responsiveness was variable, and appeared to be influenced by the strain and sex of the mice, and the dose of alpha2M given."}
{"id": "PMID:1102162", "title": "Autoimmunity to sperm antigens in vasectomized men.", "content": "Sera from vasectomized men were tested for the presence of antibodies directed against sperm antigens. A high percentage (about 55%) of the vasectomized men developed agglutinating antibodies. A lower percentage (22%) also developed low titres of antibodies to human protamine, as detected in the indirect IFT on swollen sperm heads and 22% developed cytotoxic antibodies. A correlation was found between the presence of anti-protamine antibodies and the presence of agglutinating and of cytotoxic antibodies. This correlation, and also the fact that they developed after vasectomy, indicates that the formation of antibodies against human protamine is a result of an autoimmune response to spermatozoa. The indirect IFT was also performed on 'normal' unswollen spermatozoa. All the sera were positive at least on one of the sperm antigens located in the acrosome, equator, or post-nuclear region, but no increase nor decrease in titre was found after vasectomy.", "contents": "Autoimmunity to sperm antigens in vasectomized men. Sera from vasectomized men were tested for the presence of antibodies directed against sperm antigens. A high percentage (about 55%) of the vasectomized men developed agglutinating antibodies. A lower percentage (22%) also developed low titres of antibodies to human protamine, as detected in the indirect IFT on swollen sperm heads and 22% developed cytotoxic antibodies. A correlation was found between the presence of anti-protamine antibodies and the presence of agglutinating and of cytotoxic antibodies. This correlation, and also the fact that they developed after vasectomy, indicates that the formation of antibodies against human protamine is a result of an autoimmune response to spermatozoa. The indirect IFT was also performed on 'normal' unswollen spermatozoa. All the sera were positive at least on one of the sperm antigens located in the acrosome, equator, or post-nuclear region, but no increase nor decrease in titre was found after vasectomy."}
{"id": "PMID:1102163", "title": "Observations in immunotherapy of lymphoma and melanoma patients.", "content": "Maintenance of remission solely by repeated BCG vaccinations in seven patients with non-Hodgkin's lymphoma who had achieved a complete clinical remission with initial standard therapy has provided sufficient encouragement to begin a randomized clinical trial. In vitro lymphocyte responses to mitogens and PPD used as parameters of cell-mediated immunity have not proved to be of value in predicting early or late recurrence in six pre-trial and trial patients. Eight out of twenty-one patients with malignant melanoma have shown a satisfactory clinical response (10-34 months) to immunotherapy. Those who respond must show immunological reactivity to the stimulating agent, however the best clinical responses were not associated with the highest degrees of in vivo and in vitro sensitization. The skin reactivity and the in vitro lymphocyte response to PPD as well as a 2-3-fold increase in the appearance of colony-forming units in the peripheral blood following the intratumour injection of BCG or PPD are helpful in prognosis and management of these patients. All patients with malignant melanoma who presented with a PHA response less than 40% of normal made a poor response to immunotherapy. Autopsies performed on seven patients dying with extensive melanocarcinomatous disease failed to show any serious adverse toxic reactions or infections from oral and intratumour injections of BCG.", "contents": "Observations in immunotherapy of lymphoma and melanoma patients. Maintenance of remission solely by repeated BCG vaccinations in seven patients with non-Hodgkin's lymphoma who had achieved a complete clinical remission with initial standard therapy has provided sufficient encouragement to begin a randomized clinical trial. In vitro lymphocyte responses to mitogens and PPD used as parameters of cell-mediated immunity have not proved to be of value in predicting early or late recurrence in six pre-trial and trial patients. Eight out of twenty-one patients with malignant melanoma have shown a satisfactory clinical response (10-34 months) to immunotherapy. Those who respond must show immunological reactivity to the stimulating agent, however the best clinical responses were not associated with the highest degrees of in vivo and in vitro sensitization. The skin reactivity and the in vitro lymphocyte response to PPD as well as a 2-3-fold increase in the appearance of colony-forming units in the peripheral blood following the intratumour injection of BCG or PPD are helpful in prognosis and management of these patients. All patients with malignant melanoma who presented with a PHA response less than 40% of normal made a poor response to immunotherapy. Autopsies performed on seven patients dying with extensive melanocarcinomatous disease failed to show any serious adverse toxic reactions or infections from oral and intratumour injections of BCG."}
{"id": "PMID:1102164", "title": "Reversible granulocyte killing defect in anorexia nervosa.", "content": "Three patients are described with anorexia nervosa in whom malnutrition was present with neutropenia and a granulocyte bactericidal degect. Their peripheral blood granulocytes were found to have a reduced rate of killing of Staphylococcus aureus and E. coli in vitro. The opsonic activity of the patients' sera towards Staphylococcus aureus was normal. One of these patients had recurrent episodes of infection which stopped after she had gained 13 kg in weight. Clinical recovery was associated with a return of granulocyte function to normal. It is concluded that granulocyte bactericidal capacity towards a variety of bacteria may be reduced in patients with anorexia nervosa who have malnutrition. This type of acquired granulocyte bactericidal deficiency appears to be reversible.", "contents": "Reversible granulocyte killing defect in anorexia nervosa. Three patients are described with anorexia nervosa in whom malnutrition was present with neutropenia and a granulocyte bactericidal degect. Their peripheral blood granulocytes were found to have a reduced rate of killing of Staphylococcus aureus and E. coli in vitro. The opsonic activity of the patients' sera towards Staphylococcus aureus was normal. One of these patients had recurrent episodes of infection which stopped after she had gained 13 kg in weight. Clinical recovery was associated with a return of granulocyte function to normal. It is concluded that granulocyte bactericidal capacity towards a variety of bacteria may be reduced in patients with anorexia nervosa who have malnutrition. This type of acquired granulocyte bactericidal deficiency appears to be reversible."}
{"id": "PMID:1102165", "title": "Surface markers on lymphocytes of multiple sclerosis patients.", "content": "Peripheral blood lymphocytes of twenty-two multiple sclerosis (MS) patients and thirty-five healthy controls were examined for the presence of surface markers characteristic for B lymphocytes (surface immunoglobulin, receptor for C3 (EAC), reporter for Fc (EA) and the spontaneous rosette-forming capacity characteristic of T cells. The results obtained indicate that the number of B and T cells in MS is similar to controls, as evaluated by the presence of surface immunoglobulin and E rosette-forming capacity. However, a statistically significant reduction in the percentage of lymphocytes bearing C3 receptors has been found in MS patients. It might have resulted from a reduction in the lymphocyte population bearing C3 receptor but no surface immunoglobulin. The EA rosette test revealed the greatest difference between the groups. The difference indicated a reduction in the density of the receptor for 7S Fc on lymphocytes from MS patients. The results obtained are consistent with the hypothesis of an immune deficit in multiple sclerosis.", "contents": "Surface markers on lymphocytes of multiple sclerosis patients. Peripheral blood lymphocytes of twenty-two multiple sclerosis (MS) patients and thirty-five healthy controls were examined for the presence of surface markers characteristic for B lymphocytes (surface immunoglobulin, receptor for C3 (EAC), reporter for Fc (EA) and the spontaneous rosette-forming capacity characteristic of T cells. The results obtained indicate that the number of B and T cells in MS is similar to controls, as evaluated by the presence of surface immunoglobulin and E rosette-forming capacity. However, a statistically significant reduction in the percentage of lymphocytes bearing C3 receptors has been found in MS patients. It might have resulted from a reduction in the lymphocyte population bearing C3 receptor but no surface immunoglobulin. The EA rosette test revealed the greatest difference between the groups. The difference indicated a reduction in the density of the receptor for 7S Fc on lymphocytes from MS patients. The results obtained are consistent with the hypothesis of an immune deficit in multiple sclerosis."}
{"id": "PMID:1102166", "title": "An immunofluorescence study of warts.", "content": "An investigation of the incidence of wart virus-specific antibody and of virion antigens in patients with single or multiple warts taken from different anatomical sites showed that all warts did not contain antigen. The incidence was identical using either rabbit antiserum or human sera known to contain virus-specific antibody. The warts from sole, heel and toe had a much higher incidence of stainable virus antigen. Virus-specific staining was mainly found in the keratinized and granular layers of the wart but occasional synthesis in prickle cell layer was observed. All the patients who had virus antigens in their warts did not have virus-specific antibodies but no patient who had antibody lacked antigen. Of the virus-specific antibodies, the IgM class was predominant; a smaller number also contained virus-specific IgG antibody; none had virus antigens and both classes of virus-specific antibody. A wart cell-specific IgM was also found along with virus-specific antibodies and independent of them. An IgM producing fibrillar Staining in human embryonic lung cells was noted in some sera.", "contents": "An immunofluorescence study of warts. An investigation of the incidence of wart virus-specific antibody and of virion antigens in patients with single or multiple warts taken from different anatomical sites showed that all warts did not contain antigen. The incidence was identical using either rabbit antiserum or human sera known to contain virus-specific antibody. The warts from sole, heel and toe had a much higher incidence of stainable virus antigen. Virus-specific staining was mainly found in the keratinized and granular layers of the wart but occasional synthesis in prickle cell layer was observed. All the patients who had virus antigens in their warts did not have virus-specific antibodies but no patient who had antibody lacked antigen. Of the virus-specific antibodies, the IgM class was predominant; a smaller number also contained virus-specific IgG antibody; none had virus antigens and both classes of virus-specific antibody. A wart cell-specific IgM was also found along with virus-specific antibodies and independent of them. An IgM producing fibrillar Staining in human embryonic lung cells was noted in some sera."}
{"id": "PMID:1102167", "title": "Smooth muscle antibody in patients with warts.", "content": "Sera from fifty-four patients with warts were tested for the presence of commonly found autoantibodies. Smooth muscle antibody (SMA) was detected at a significantly higher level than in a control group of healthy blood donors matched for age and sex, and was in the IgM class. The SMA-positive sera gave a microfilamentous staining pattern also in the IgM class, on fixed HEp2 tissue culture cells. Absorption of positive sera with smooth muscle removed both SMA staining and the anti-cellular staining.", "contents": "Smooth muscle antibody in patients with warts. Sera from fifty-four patients with warts were tested for the presence of commonly found autoantibodies. Smooth muscle antibody (SMA) was detected at a significantly higher level than in a control group of healthy blood donors matched for age and sex, and was in the IgM class. The SMA-positive sera gave a microfilamentous staining pattern also in the IgM class, on fixed HEp2 tissue culture cells. Absorption of positive sera with smooth muscle removed both SMA staining and the anti-cellular staining."}
{"id": "PMID:1102168", "title": "Hypochlorite-induced alterations to canine serum complement.", "content": "Changes in the concentration of the components of complement produced by NaOC1 both in vitro and in vivo are recorded. C1, C4 and C7 are particularly sensitive to this oxidizing agent, although all components decrease at high concentrations of NaOC1. Following oxidation, complement componenets return rapidly to normal. Data are presented to indicate that part of this repair mechanism is due to the action of reducing agents such as ascorbic acid and part is due to the synthesis of the individual components. The unique sensitivity of complement components to oxidation make this treatment of potential value in suppressing the inflammatory response.", "contents": "Hypochlorite-induced alterations to canine serum complement. Changes in the concentration of the components of complement produced by NaOC1 both in vitro and in vivo are recorded. C1, C4 and C7 are particularly sensitive to this oxidizing agent, although all components decrease at high concentrations of NaOC1. Following oxidation, complement componenets return rapidly to normal. Data are presented to indicate that part of this repair mechanism is due to the action of reducing agents such as ascorbic acid and part is due to the synthesis of the individual components. The unique sensitivity of complement components to oxidation make this treatment of potential value in suppressing the inflammatory response."}
{"id": "PMID:1102175", "title": "Renal cystic disease associated with tuberous sclerosis complex: renal failure treated by cadaveric kidney transplantation.", "content": "Chronic renal failure in patients with tuberous sclerosis may be secondary to diffuse cystic disease, a lesion less common than the better known hamartomatous angiomyolipomas. Uremia, in the case of a nineteen-year old female with end-stage renal disease, was associated with severely atrophic kidneys that contained numerous collapsed and scarred cysts. No hamartomas were present. The patient survived for more than three years following cadaveric renal transplantation.", "contents": "Renal cystic disease associated with tuberous sclerosis complex: renal failure treated by cadaveric kidney transplantation. Chronic renal failure in patients with tuberous sclerosis may be secondary to diffuse cystic disease, a lesion less common than the better known hamartomatous angiomyolipomas. Uremia, in the case of a nineteen-year old female with end-stage renal disease, was associated with severely atrophic kidneys that contained numerous collapsed and scarred cysts. No hamartomas were present. The patient survived for more than three years following cadaveric renal transplantation."}
{"id": "PMID:1102177", "title": "Investigations on Leydig cell function and regulation after renal transplantation.", "content": "The hypothalamic-hypophyseal-Leydig cell axis was investigated in 17 male patients following renal transplantation. Plasma levels of testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) were determined by radioimmunoassay under basal conditions and after stimulation with chorionic gonadotrophin (HCG) and hypothalamic releasing hormone (LH/FSH-RH) respectively. The investigations were carried out one to five years after renal transplantation. It was demonstrated, that primary dysfunction of Leydig cells may occur after renal transplantation. In some cases however normal Leydig cell function with adequate testosterone secretion may return. It remains uncertain, whether the Leydig cell dysfunction is a result of preexisting toxic uremic damage or whether it is due to immunosuppressive therapy with prednisone and azathioprine.", "contents": "Investigations on Leydig cell function and regulation after renal transplantation. The hypothalamic-hypophyseal-Leydig cell axis was investigated in 17 male patients following renal transplantation. Plasma levels of testosterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) were determined by radioimmunoassay under basal conditions and after stimulation with chorionic gonadotrophin (HCG) and hypothalamic releasing hormone (LH/FSH-RH) respectively. The investigations were carried out one to five years after renal transplantation. It was demonstrated, that primary dysfunction of Leydig cells may occur after renal transplantation. In some cases however normal Leydig cell function with adequate testosterone secretion may return. It remains uncertain, whether the Leydig cell dysfunction is a result of preexisting toxic uremic damage or whether it is due to immunosuppressive therapy with prednisone and azathioprine."}
{"id": "PMID:1102178", "title": "Fetal haemoglobin in homozygous sickle cell disease.", "content": "Synthesis of fetal haemoglobin is prolonged in homozygous sickle cell disease. Its intracellular distribution is irregular and red cells containing high levels of Hb F enjoy greater protection from sickling and subsequent destruction. This relationship is reflected in a number of associations between Hb F level and the haematological and clinical parameters of the disease. High fetal haemoglobin levels are associated with more normal red cell survival, more normal oxygen affinity, and more normal red cell characteristics whereas clinically they are associated with less evidence of vessel obstruction, persistence of splenomegaly, more normal skeletal development and body habitus, and a generally more benign clinical course. Attempts to prolong Hb F synthesis might therefore be expected to lead to amelioration of the clinical features of the disease. The factors leading to persistence of Hb F synthesis is SS disease are largely unknown although they appear to be operative as early as the first three months of life.", "contents": "Fetal haemoglobin in homozygous sickle cell disease. Synthesis of fetal haemoglobin is prolonged in homozygous sickle cell disease. Its intracellular distribution is irregular and red cells containing high levels of Hb F enjoy greater protection from sickling and subsequent destruction. This relationship is reflected in a number of associations between Hb F level and the haematological and clinical parameters of the disease. High fetal haemoglobin levels are associated with more normal red cell survival, more normal oxygen affinity, and more normal red cell characteristics whereas clinically they are associated with less evidence of vessel obstruction, persistence of splenomegaly, more normal skeletal development and body habitus, and a generally more benign clinical course. Attempts to prolong Hb F synthesis might therefore be expected to lead to amelioration of the clinical features of the disease. The factors leading to persistence of Hb F synthesis is SS disease are largely unknown although they appear to be operative as early as the first three months of life."}
{"id": "PMID:1102181", "title": "Immune haemolytic disease: the autoimmune haemolytic anaemias.", "content": "The autoimmune haemolytic anaemias are common syndromes, with protean clinical features reflecting a variety of significant associated diseases. A diagnosis of this state should alert the clinician to the possibility of an aberrant immune mechanism. Years may elapse between development of the haemolytic process and the eventurl emergence of the entire disease pattern. The haemolytic anaemia should be considered as the easily diagnosed part of a complex, multisystem disease resulting from malfunction of the immune apparatus. Therapy can be exceedingly difficult. The following outline is suggested as a general approach: 1. Start prednisone 60 mg daily. If a therapeutic response occurs, continue this dosage until the haematocrit reaches 30 per cent. A slow but progressive reduction should then be initiated. 2. If prednisone dosages greater than 15 mg daily are required to maintain the remission, treat as a therapeutic failure. 3. If no response occurs after one week of prednisone, start azathioprine 2.0 to 2.5 mg/kg. 4. If no response is apparent after two additional weeks (three weeks of prednisone), progressively reduce and eventually discontinue prednisone. 5. If no response occurs after a total of four weeks of azathioprine, one of two alternative therapies should be started: (a) perform splenectomy, or (b) increase azathioprine by 25 mg daily, every one to two weeks, until either a response occurs or reduced bone marrow function is observed. 6. If azathioprine and splenectomy both fail, experimental therapies such as antithymocyte antiserum or thymectomy should be considered. 7. Transfusions are to be used only as temporary paliation in life-threatening neurological or cardiovascular complications.", "contents": "Immune haemolytic disease: the autoimmune haemolytic anaemias. The autoimmune haemolytic anaemias are common syndromes, with protean clinical features reflecting a variety of significant associated diseases. A diagnosis of this state should alert the clinician to the possibility of an aberrant immune mechanism. Years may elapse between development of the haemolytic process and the eventurl emergence of the entire disease pattern. The haemolytic anaemia should be considered as the easily diagnosed part of a complex, multisystem disease resulting from malfunction of the immune apparatus. Therapy can be exceedingly difficult. The following outline is suggested as a general approach: 1. Start prednisone 60 mg daily. If a therapeutic response occurs, continue this dosage until the haematocrit reaches 30 per cent. A slow but progressive reduction should then be initiated. 2. If prednisone dosages greater than 15 mg daily are required to maintain the remission, treat as a therapeutic failure. 3. If no response occurs after one week of prednisone, start azathioprine 2.0 to 2.5 mg/kg. 4. If no response is apparent after two additional weeks (three weeks of prednisone), progressively reduce and eventually discontinue prednisone. 5. If no response occurs after a total of four weeks of azathioprine, one of two alternative therapies should be started: (a) perform splenectomy, or (b) increase azathioprine by 25 mg daily, every one to two weeks, until either a response occurs or reduced bone marrow function is observed. 6. If azathioprine and splenectomy both fail, experimental therapies such as antithymocyte antiserum or thymectomy should be considered. 7. Transfusions are to be used only as temporary paliation in life-threatening neurological or cardiovascular complications."}
{"id": "PMID:1102205", "title": "Monitoring of patients with head injuries.", "content": "A practical protocol for evaluating and monitoring head injured patients has been presented. It has been stressed that monitoring is an ongoing process which begins with the initial evaluation and continues throughout the patient's hospital stay. The level of monitoring and thus the parameters to be monitored are determined on an individualized basis according to the patient's clinical status and his level of stupor and coma. It has been suggested that for mild head injuries, adequate monitoring includes periodic evaluation of the neurological status and vital signs; but the more severely injured patients require extensive and frequent monitoring of a large number of clinical and physiological parameters. Among the many parameters, the continuous monitoring of ICP and the respiratory system are perhaps the most useful indicators of the patient's condition. Several methods for continuous ICP monitoring which are readily adaptable to most neurosurgical practices have been discussed. These include the intraventricular catheter, subarachnoid screw, and the closed Rickham reservoir connected to a ventricular catheter. In our institution, the Rickham reservoir has been used to monitor ICP and at the time of this report, satisfactory ICP recordings have been obtained in 27 of 30 head injury patients. Although this method requires a small surgical procedure, we believe that the decreased incidence of infection, the rapid access to ventricular fluid, and its potential for long term monitoring justify its use. Suggestions for monitoring other physiological parameters have also been presented. Particular emphasis has been placed on the management of infants and children with head injuries. The clinical entity of chronic subdural hematoma in infants and children has been used to illustrate the value of ICP monitoring as a guide to further treatment. Finally, we have briefly discussed the place of computers in day to day patient care, research, and monitoring.", "contents": "Monitoring of patients with head injuries. A practical protocol for evaluating and monitoring head injured patients has been presented. It has been stressed that monitoring is an ongoing process which begins with the initial evaluation and continues throughout the patient's hospital stay. The level of monitoring and thus the parameters to be monitored are determined on an individualized basis according to the patient's clinical status and his level of stupor and coma. It has been suggested that for mild head injuries, adequate monitoring includes periodic evaluation of the neurological status and vital signs; but the more severely injured patients require extensive and frequent monitoring of a large number of clinical and physiological parameters. Among the many parameters, the continuous monitoring of ICP and the respiratory system are perhaps the most useful indicators of the patient's condition. Several methods for continuous ICP monitoring which are readily adaptable to most neurosurgical practices have been discussed. These include the intraventricular catheter, subarachnoid screw, and the closed Rickham reservoir connected to a ventricular catheter. In our institution, the Rickham reservoir has been used to monitor ICP and at the time of this report, satisfactory ICP recordings have been obtained in 27 of 30 head injury patients. Although this method requires a small surgical procedure, we believe that the decreased incidence of infection, the rapid access to ventricular fluid, and its potential for long term monitoring justify its use. Suggestions for monitoring other physiological parameters have also been presented. Particular emphasis has been placed on the management of infants and children with head injuries. The clinical entity of chronic subdural hematoma in infants and children has been used to illustrate the value of ICP monitoring as a guide to further treatment. Finally, we have briefly discussed the place of computers in day to day patient care, research, and monitoring."}
{"id": "PMID:1102210", "title": "Volume and pressure in the craniospinal axis.", "content": "Raised intracranial pressure is common in neurosurgical practice, but the clinical effects are variable. The factors which initiate intracranial hypertension are now fairly well established; the extent of the increase of intracranial pressure produced by any combination of these factors is a function of the volume-pressure status of the patient at that particular time and may be expressed in terms of brain clastance. The effects of raised intracranial pressure on the function of the brain are mediated through both changes in cerebral blood flow and brain shift or herniation. Understanding of these principles helps in selection of effective therapy for raised intracranial pressure. Ideal clinical management of raised intracranial pressure should be directed toward the identification of those situations in which intracranial pressure may become increased and should attempt to prevent intracranial hypertension before it actually occurs.", "contents": "Volume and pressure in the craniospinal axis. Raised intracranial pressure is common in neurosurgical practice, but the clinical effects are variable. The factors which initiate intracranial hypertension are now fairly well established; the extent of the increase of intracranial pressure produced by any combination of these factors is a function of the volume-pressure status of the patient at that particular time and may be expressed in terms of brain clastance. The effects of raised intracranial pressure on the function of the brain are mediated through both changes in cerebral blood flow and brain shift or herniation. Understanding of these principles helps in selection of effective therapy for raised intracranial pressure. Ideal clinical management of raised intracranial pressure should be directed toward the identification of those situations in which intracranial pressure may become increased and should attempt to prevent intracranial hypertension before it actually occurs."}
{"id": "PMID:1102211", "title": "Phenobarbital plasma levels in neonates.", "content": "This pilot study demonstrated marked variation in neonatal phenobarbital utilization. In order to rapidly achieve therapeutic levels, it is suggested that a loading dose of 8 to 10 mg per kg be administered for 2 days followed by reduction of dosage to a maintenance level of 5 to 6 mg per kg with frequent monitoring of plasma phenobarbital concentrations. The optimal plasma concentration of phenobarbital for control of neonatal seizures or withdrawal syndromes appeared to be between 15 to 30 mug per ml.", "contents": "Phenobarbital plasma levels in neonates. This pilot study demonstrated marked variation in neonatal phenobarbital utilization. In order to rapidly achieve therapeutic levels, it is suggested that a loading dose of 8 to 10 mg per kg be administered for 2 days followed by reduction of dosage to a maintenance level of 5 to 6 mg per kg with frequent monitoring of plasma phenobarbital concentrations. The optimal plasma concentration of phenobarbital for control of neonatal seizures or withdrawal syndromes appeared to be between 15 to 30 mug per ml."}
{"id": "PMID:1102216", "title": "Digoxin: placental transfer, effects on the fetus, and therapeutic use in the newborn.", "content": "Digoxin rapidly crosses the placenta and reaches equilibrium, with maternal and fetal sera having equal concentrations. Virtually nothing is known about the effects of transplacentally administered digoxin on the fetus. Toxicity has been reported in the fetus of a woman ingesting a huge overdose of digitoxin; the same result would be anticipated with digoxin poisoning. Serum levels in pregnant women receiving the standard dose of 0.25 mg tend to be subnormal and certain patients may require a small increase in dose during the last trimester. While the full-term neonate appears to tolerate relatively high doses and the resultant high serum levels, there is no compelling evidence that such doses are necessary or even useful. Since toxicity can and does occur in neonates, especially during administration of loading (digitalizing) doses, it is recommended that maintenance doses of 0.01 mg per kg per day be used routinely. If the full inotropic effect is needed immediately, a loading dose of 0.03 mg per kg may be employed. Maintenance therapy is then begun on the following day. Without a loading dose cumulation occurs for about 3 days; after 5 or so days, serum levels will equal those found after use of a loading dose followed by maintenance therapy. Results of a single study suggest that the daily dose should be divided and given every 12 hours. After about 1 week of therapy, the serum level should be determined and the dose modified to maintain a serum level of 1 to 2 ng per ml. If the therapeutic effect is less than desired, a cautions increase in dose to as high as 0.02 mg per kg per day or to that dose which produces serum levels up to 3 ng per ml can be tried. Certain infants appear to tolerate serum levels of 3.5 to 4 ng per ml but such infants must be closely monitored. There are no data which indicate that a greater inotropic response will occur at these high serum levels, though this point has not been definitively investigated, and is the highest priority question for research. The intramuscular route should be researved for the unusual situation. Vomiting should be considered an early sign of toxicity and may act as a \"safety valve.\" When adminstered in solution (as in the elixir or solution for intravenous use), oral digoxin is rapidly absorbed an an inotropic response is found within minutes, reaching a peak within hours, so that little is gained by parenteral administration. If an inotropic effect is urgently needed, intravenous administration of ouabain will give an immediate response.", "contents": "Digoxin: placental transfer, effects on the fetus, and therapeutic use in the newborn. Digoxin rapidly crosses the placenta and reaches equilibrium, with maternal and fetal sera having equal concentrations. Virtually nothing is known about the effects of transplacentally administered digoxin on the fetus. Toxicity has been reported in the fetus of a woman ingesting a huge overdose of digitoxin; the same result would be anticipated with digoxin poisoning. Serum levels in pregnant women receiving the standard dose of 0.25 mg tend to be subnormal and certain patients may require a small increase in dose during the last trimester. While the full-term neonate appears to tolerate relatively high doses and the resultant high serum levels, there is no compelling evidence that such doses are necessary or even useful. Since toxicity can and does occur in neonates, especially during administration of loading (digitalizing) doses, it is recommended that maintenance doses of 0.01 mg per kg per day be used routinely. If the full inotropic effect is needed immediately, a loading dose of 0.03 mg per kg may be employed. Maintenance therapy is then begun on the following day. Without a loading dose cumulation occurs for about 3 days; after 5 or so days, serum levels will equal those found after use of a loading dose followed by maintenance therapy. Results of a single study suggest that the daily dose should be divided and given every 12 hours. After about 1 week of therapy, the serum level should be determined and the dose modified to maintain a serum level of 1 to 2 ng per ml. If the therapeutic effect is less than desired, a cautions increase in dose to as high as 0.02 mg per kg per day or to that dose which produces serum levels up to 3 ng per ml can be tried. Certain infants appear to tolerate serum levels of 3.5 to 4 ng per ml but such infants must be closely monitored. There are no data which indicate that a greater inotropic response will occur at these high serum levels, though this point has not been definitively investigated, and is the highest priority question for research. The intramuscular route should be researved for the unusual situation. Vomiting should be considered an early sign of toxicity and may act as a \"safety valve.\" When adminstered in solution (as in the elixir or solution for intravenous use), oral digoxin is rapidly absorbed an an inotropic response is found within minutes, reaching a peak within hours, so that little is gained by parenteral administration. If an inotropic effect is urgently needed, intravenous administration of ouabain will give an immediate response."}
{"id": "PMID:1102222", "title": "Protein metabolism during pregnancy.", "content": "Protein metabolism is altered during pregnancy to allow for an increased rate of tissue synthesis. These adjustments seem to be mediated primarily by hormonal changes. The observed nitrogen, and therefore protein, retention is in the order of 1.1 gm N per day during the last half of pregnancy. The theoretical gain of nitrogen in fetal and maternal accessory tissues is about 0.8 gm per day during the same period. Animal data and a few human studies suggest that the difference may be accounted for by maternal gain of fat-free tissue. The rate of nitrogen retention appears to increase by a factor of 3 from early to late pregnancy. The current NRC protein recommendation for pregnancy is 30 gm additional protein or 1.3 gm per kg pregnant body weight. This allowance takes into consideration the low efficiency of protein utilization in the practical diets consumed by most pregnant women. The recommendation is made assuming that energy needs have been met. Within reasonable limits increased energy intakes will improve protein utilization. Some clinical surveys have noted a relationship between prenatal diet and infant size at birth, infant health in early life, pregnancy complications, and fertility rate; others have failed to find these associations. Supplements of food given to pregnant women in an attempt to improve outcome are associated with significant improvement in poorly nourished women but less impact in more well nourished women. When healthy pregnant women eat to appetite, the protein and energy intakes seem to be comparable to the current NRC recommendations.", "contents": "Protein metabolism during pregnancy. Protein metabolism is altered during pregnancy to allow for an increased rate of tissue synthesis. These adjustments seem to be mediated primarily by hormonal changes. The observed nitrogen, and therefore protein, retention is in the order of 1.1 gm N per day during the last half of pregnancy. The theoretical gain of nitrogen in fetal and maternal accessory tissues is about 0.8 gm per day during the same period. Animal data and a few human studies suggest that the difference may be accounted for by maternal gain of fat-free tissue. The rate of nitrogen retention appears to increase by a factor of 3 from early to late pregnancy. The current NRC protein recommendation for pregnancy is 30 gm additional protein or 1.3 gm per kg pregnant body weight. This allowance takes into consideration the low efficiency of protein utilization in the practical diets consumed by most pregnant women. The recommendation is made assuming that energy needs have been met. Within reasonable limits increased energy intakes will improve protein utilization. Some clinical surveys have noted a relationship between prenatal diet and infant size at birth, infant health in early life, pregnancy complications, and fertility rate; others have failed to find these associations. Supplements of food given to pregnant women in an attempt to improve outcome are associated with significant improvement in poorly nourished women but less impact in more well nourished women. When healthy pregnant women eat to appetite, the protein and energy intakes seem to be comparable to the current NRC recommendations."}
{"id": "PMID:1102224", "title": "Vitamin passage across the placenta.", "content": "A pregnant woman who has adequate nutrition available in the form of a standard diet will provide the vitamins required by the fetus for normal development. Active transport of the water-soluble vitamins plus easy oral absorption after birth insure nutritional sufficiency at any gestational age. The fat-soluble vitamins which have a much slower transfer rate as the result of facilitated diffusion do not achieve the same degree of storage in the developing fetus. Thus, the premature infant who has decreased stores and who may have impaired oral absorption initially may become deficient in a fat-soluble vitamin (A, D, E, K) during the first 4 to 6 weeks of life. Oral or possibly parenteral supplementation following birth, however, would seem more appropriate than subtoxic doses administered to the mother prenatally.", "contents": "Vitamin passage across the placenta. A pregnant woman who has adequate nutrition available in the form of a standard diet will provide the vitamins required by the fetus for normal development. Active transport of the water-soluble vitamins plus easy oral absorption after birth insure nutritional sufficiency at any gestational age. The fat-soluble vitamins which have a much slower transfer rate as the result of facilitated diffusion do not achieve the same degree of storage in the developing fetus. Thus, the premature infant who has decreased stores and who may have impaired oral absorption initially may become deficient in a fat-soluble vitamin (A, D, E, K) during the first 4 to 6 weeks of life. Oral or possibly parenteral supplementation following birth, however, would seem more appropriate than subtoxic doses administered to the mother prenatally."}
{"id": "PMID:1102226", "title": "Nutrition for the low birth weight infant.", "content": "Satisfactory nutrition of the low birth weight infant concerns the nurse, obstetrician, pediatrician, and nutritionist. It may be that at present the most satisfactory feeding for the low birth weight infants is human milk. Such factors as protein quantity and quality, fat quantity and quality, immune factors, and osmolar factors may be uniquely suitable for the human infant regardless of his weight. Because of present limitations in the availability of fresh human milk, infant formulas have been adopted with reasonable success in the nutriture of the low birth weight infant. Those formulas which most nearly simulate human milk are most widely used. Broad outlines for the composition of these formulas have been given. Regardless of the availability and ease of administration of either human milk or formula, it is unlikely that any form of external nutriture can equal the advantages provided by intrauterine growth. Until better methods are devised for prolonging intrauterine growth, presently available feeding regimens should be considered experimental and in a state of flux.", "contents": "Nutrition for the low birth weight infant. Satisfactory nutrition of the low birth weight infant concerns the nurse, obstetrician, pediatrician, and nutritionist. It may be that at present the most satisfactory feeding for the low birth weight infants is human milk. Such factors as protein quantity and quality, fat quantity and quality, immune factors, and osmolar factors may be uniquely suitable for the human infant regardless of his weight. Because of present limitations in the availability of fresh human milk, infant formulas have been adopted with reasonable success in the nutriture of the low birth weight infant. Those formulas which most nearly simulate human milk are most widely used. Broad outlines for the composition of these formulas have been given. Regardless of the availability and ease of administration of either human milk or formula, it is unlikely that any form of external nutriture can equal the advantages provided by intrauterine growth. Until better methods are devised for prolonging intrauterine growth, presently available feeding regimens should be considered experimental and in a state of flux."}
{"id": "PMID:1102228", "title": "Nutritional management of infants with inborn metabolic errors.", "content": "Inborn errors of metabolism can dramatically manifest themselves in the new born period or remain completely undetected. Awareness on the part of the physician, with every attempt being made to arrive at an early diagnosis, is the basis for a favorable outcome. Many approaches to therapy are possible but the most frequently used is that of substrate restriction in the form of dietary control. Frequent monitoring of growth as well as of plasma biochemical analysis can result in adequate nutritional status as well as in normal development.", "contents": "Nutritional management of infants with inborn metabolic errors. Inborn errors of metabolism can dramatically manifest themselves in the new born period or remain completely undetected. Awareness on the part of the physician, with every attempt being made to arrive at an early diagnosis, is the basis for a favorable outcome. Many approaches to therapy are possible but the most frequently used is that of substrate restriction in the form of dietary control. Frequent monitoring of growth as well as of plasma biochemical analysis can result in adequate nutritional status as well as in normal development."}
{"id": "PMID:1102229", "title": "Infant nutrition.", "content": "Although most physicians agree that human milk is superior to artificial feeding, the latter is far more popular in well developed countries. There are various reasons for the decrease in breast feeding but a major factor has been the introduction of a variety of simple, safe milk and milk-substitute formulas. The healthy full-term infant will thrive on any of a number of properly prepared formulas as well as human milk. Mothers should be encouraged to breast feed by the obstetrician and pediatrician, but should not be made to feel guilty if they do not. Regardless of the method used for feeding, proper instruction is mandatory. A practical approach to the management of breast feeding is presented, as is a guide to artificial feeding. A brief review of the full-term infant's nutritional requirements is given together with a comparison of human milk, cow's milk, and commercially prepared feeding products. The availability and use of the specialized formulas is also included.", "contents": "Infant nutrition. Although most physicians agree that human milk is superior to artificial feeding, the latter is far more popular in well developed countries. There are various reasons for the decrease in breast feeding but a major factor has been the introduction of a variety of simple, safe milk and milk-substitute formulas. The healthy full-term infant will thrive on any of a number of properly prepared formulas as well as human milk. Mothers should be encouraged to breast feed by the obstetrician and pediatrician, but should not be made to feel guilty if they do not. Regardless of the method used for feeding, proper instruction is mandatory. A practical approach to the management of breast feeding is presented, as is a guide to artificial feeding. A brief review of the full-term infant's nutritional requirements is given together with a comparison of human milk, cow's milk, and commercially prepared feeding products. The availability and use of the specialized formulas is also included."}
{"id": "PMID:1102230", "title": "Increased arachidonate in lipids after administration to man: effects on prostaglandin biosynthesis.", "content": "Ethyl arachidonate was administered orally to 4 healthy male volunteers in a dose of 6 gm daily for a 2 to 3 wk period after 10-day control period. The increased intake of this precursor of the dienoic prostaglandins resulted in significant increases in the relative and absolute amount of arachidonate in plasma triglycerides, phospholipids, and cholesteryl esters. Similar changes in lipid composition were noted in platelets. The excretion of 7alpha-hydroxy-5,11-diketotetranoprostane-1,16-dioic acid, the major urinary metabolite of E prostaglandins in man, was increased by an average of 47% in 3 of the 4 volunteers. Platelet reactivity was assessed by determining the threshold concentration of adenosine diphosphate (ADP) necessary to induce secondary, irreversible aggregation of platelet-rich plasma. This threshold concentration dropped significantly in all volunteers (10% to 60% of control values). It is concluded that the biosynthesis and function of prostaglandins can be augmented in man by oral administration of an esterified precursor fatty acid.", "contents": "Increased arachidonate in lipids after administration to man: effects on prostaglandin biosynthesis. Ethyl arachidonate was administered orally to 4 healthy male volunteers in a dose of 6 gm daily for a 2 to 3 wk period after 10-day control period. The increased intake of this precursor of the dienoic prostaglandins resulted in significant increases in the relative and absolute amount of arachidonate in plasma triglycerides, phospholipids, and cholesteryl esters. Similar changes in lipid composition were noted in platelets. The excretion of 7alpha-hydroxy-5,11-diketotetranoprostane-1,16-dioic acid, the major urinary metabolite of E prostaglandins in man, was increased by an average of 47% in 3 of the 4 volunteers. Platelet reactivity was assessed by determining the threshold concentration of adenosine diphosphate (ADP) necessary to induce secondary, irreversible aggregation of platelet-rich plasma. This threshold concentration dropped significantly in all volunteers (10% to 60% of control values). It is concluded that the biosynthesis and function of prostaglandins can be augmented in man by oral administration of an esterified precursor fatty acid."}
{"id": "PMID:1102231", "title": "Nefopam and morphine in man.", "content": "A new analgesic, nefopam, is chemically distinct and pharmacologically unrelated to any presently known analgesic. A comparison was made of morphine and nefopam in 74 patients who required parenteral analgesia for moderate to severe postoperative and somatic pain, using a single administration, 2-dose level, double-blind design. A significant dose-response curve was obtained with nefopam and with morphine, and there was no significant deviation from parallelism. The time-effect curves for the 2 drugs were similar. The estimated relative potency of nefopam to morphine indicates that 20 mg of nefopam HCl is the approximate analgesic equal of 12 mg of morphine SO4. There were no adverse effects with nefopam and one adverse reaction to morphine.", "contents": "Nefopam and morphine in man. A new analgesic, nefopam, is chemically distinct and pharmacologically unrelated to any presently known analgesic. A comparison was made of morphine and nefopam in 74 patients who required parenteral analgesia for moderate to severe postoperative and somatic pain, using a single administration, 2-dose level, double-blind design. A significant dose-response curve was obtained with nefopam and with morphine, and there was no significant deviation from parallelism. The time-effect curves for the 2 drugs were similar. The estimated relative potency of nefopam to morphine indicates that 20 mg of nefopam HCl is the approximate analgesic equal of 12 mg of morphine SO4. There were no adverse effects with nefopam and one adverse reaction to morphine."}
{"id": "PMID:1102232", "title": "Butorphanol and pentazocine in patients with severe postoperative pain.", "content": "Either butorphanol tartrate (1, 2, or 4 mg) or pentazocine (30 or 60 mg) was given intramuscularly (5 dose groups) to 262 patients, who were scheduled for major operations, if they had severe pain after full awakening in the recovery room, postoperatively under double-blind conditions. There were at least 50 patients in each of the five dosage groups. Pain intensity and relief were scored numerically for each treated patient at 1/2 1, 2, 3 and 4 hr, while under direct surveillance, and the patients were seen again for follow-ups during the first 24 hr postoperatively. Appreciable pain relief developed within 30 min at all dose levels, with a peak analgesic effect apparent at about 1 hr. Satisfactory relief persisted for 4 hr in the majority of patients in each group. With the lowest dose of butorphanol tartrate (1 mg), which gave good pain relief for only 2 to 4 hr, approximately 60% of the patients had to be remedicated within 4 hr. Vital signs were not appreciably affected, but the patients who received the high dose of analgesics (butorphanol tartrate 4 mg or pentazocine lactate 60 mg) were often quite drowsy. The incidence of other side effects was negligible. The relative potency assay showed that butorphanol was approximately 20 times as potent as pentazocine for up to 4 hr.", "contents": "Butorphanol and pentazocine in patients with severe postoperative pain. Either butorphanol tartrate (1, 2, or 4 mg) or pentazocine (30 or 60 mg) was given intramuscularly (5 dose groups) to 262 patients, who were scheduled for major operations, if they had severe pain after full awakening in the recovery room, postoperatively under double-blind conditions. There were at least 50 patients in each of the five dosage groups. Pain intensity and relief were scored numerically for each treated patient at 1/2 1, 2, 3 and 4 hr, while under direct surveillance, and the patients were seen again for follow-ups during the first 24 hr postoperatively. Appreciable pain relief developed within 30 min at all dose levels, with a peak analgesic effect apparent at about 1 hr. Satisfactory relief persisted for 4 hr in the majority of patients in each group. With the lowest dose of butorphanol tartrate (1 mg), which gave good pain relief for only 2 to 4 hr, approximately 60% of the patients had to be remedicated within 4 hr. Vital signs were not appreciably affected, but the patients who received the high dose of analgesics (butorphanol tartrate 4 mg or pentazocine lactate 60 mg) were often quite drowsy. The incidence of other side effects was negligible. The relative potency assay showed that butorphanol was approximately 20 times as potent as pentazocine for up to 4 hr."}
{"id": "PMID:1102233", "title": "Oral benzquinamide in the treatment of nausea and vomiting.", "content": "The clinical antiemetic effect of bezquinamide by oral route at a dosage of 100 mg 3 times daily was evaluated by a controlled double-blind method in 183 studies of patients treated with 5-fluorouracil. The incidence of nausea and vomiting in benzquinamide-treated patients was equal to that in placebo-treated patients and significantly higher than that in patients treated with prochlorperazine, 10 mg 3 times daily. Sedation was the only significant side effect observed, and this occurred at essentially equal rates in benzquinamide- and prochlorperazine-treated patients.", "contents": "Oral benzquinamide in the treatment of nausea and vomiting. The clinical antiemetic effect of bezquinamide by oral route at a dosage of 100 mg 3 times daily was evaluated by a controlled double-blind method in 183 studies of patients treated with 5-fluorouracil. The incidence of nausea and vomiting in benzquinamide-treated patients was equal to that in placebo-treated patients and significantly higher than that in patients treated with prochlorperazine, 10 mg 3 times daily. Sedation was the only significant side effect observed, and this occurred at essentially equal rates in benzquinamide- and prochlorperazine-treated patients."}
{"id": "PMID:1102234", "title": "A comparison of fenfluramine and amphetamine in man.", "content": "dl-Fenfluramine hydrochloride (60, 120, 240 mg), d-amphetamine sulfate (20, 40 mg), and placebo were compared in 8 postaddict volunteers, each dose given orally in random sequence at weekly intervals using a double-blind crossover design. Fenfluramine had little effect on blood pressure and temperature, but caused a marked dilation of pupils, whereas amphetamine was a potent vasopressor and a weak mydriatic. While fenfluramine produced euphoria in some subjects, its overall effects were unpleasant, sedative, and qualitatively different from amphetamine. Three subjects given 240 mg of fenfluramine experienced brief but vivid hallucinogenic episodes characterized by olfactory, visual, and somatic hallucinations, abrupt polar changes in mood, time distortion, fleeting paranoia, and sexual ideation. These observations indicate that fenfluramine is a hallucinogenic agent with a pharmacologic profile in man that is not amphetamine-like.", "contents": "A comparison of fenfluramine and amphetamine in man. dl-Fenfluramine hydrochloride (60, 120, 240 mg), d-amphetamine sulfate (20, 40 mg), and placebo were compared in 8 postaddict volunteers, each dose given orally in random sequence at weekly intervals using a double-blind crossover design. Fenfluramine had little effect on blood pressure and temperature, but caused a marked dilation of pupils, whereas amphetamine was a potent vasopressor and a weak mydriatic. While fenfluramine produced euphoria in some subjects, its overall effects were unpleasant, sedative, and qualitatively different from amphetamine. Three subjects given 240 mg of fenfluramine experienced brief but vivid hallucinogenic episodes characterized by olfactory, visual, and somatic hallucinations, abrupt polar changes in mood, time distortion, fleeting paranoia, and sexual ideation. These observations indicate that fenfluramine is a hallucinogenic agent with a pharmacologic profile in man that is not amphetamine-like."}
{"id": "PMID:1102235", "title": "Duration of hydralazine action in hypertension.", "content": "The effect on blood pressure of giving hydralazine orally, 300 mg per day divided into 2, 3, and 4 doses, was studied in 4 hypertensive patients. There was no significant difference in average mean arterial pressure for the 3 regimens. Fluctuations of mean arterial pressure with time were not significantly different for the regimens as indicated by coefficient of variation. The mean coefficient of variation for the 2 days prior to initiation of hydralazine dosing was 4.0% +/- 0.3 (SEM) and for the 3 dosing regimens ranged from 4.1% to 4.8%. Heart rate was used as an index of vasodilator-induced increase in sympathetic tone and therefore as a measure of possible side effects. Fluctuations in heart rate were small and were not associated with symptoms. After discontinuation of hydralazine, the time for blood pressure to return halfway between initial placebo value and end treatment value varied from 30 to 140 hr. A tracer dose of hydralazine 14C. HCl was administered intravenously to the patients. The urinary excretion of 14C showed a multiexponential pattern of elimination with a previously undescribed prolonged terminal phase. Under the conditions of the present study, a daily dose of 300 mg of hydralazine was as effective and free of side effects given in 2 as in 4 divided doses.", "contents": "Duration of hydralazine action in hypertension. The effect on blood pressure of giving hydralazine orally, 300 mg per day divided into 2, 3, and 4 doses, was studied in 4 hypertensive patients. There was no significant difference in average mean arterial pressure for the 3 regimens. Fluctuations of mean arterial pressure with time were not significantly different for the regimens as indicated by coefficient of variation. The mean coefficient of variation for the 2 days prior to initiation of hydralazine dosing was 4.0% +/- 0.3 (SEM) and for the 3 dosing regimens ranged from 4.1% to 4.8%. Heart rate was used as an index of vasodilator-induced increase in sympathetic tone and therefore as a measure of possible side effects. Fluctuations in heart rate were small and were not associated with symptoms. After discontinuation of hydralazine, the time for blood pressure to return halfway between initial placebo value and end treatment value varied from 30 to 140 hr. A tracer dose of hydralazine 14C. HCl was administered intravenously to the patients. The urinary excretion of 14C showed a multiexponential pattern of elimination with a previously undescribed prolonged terminal phase. Under the conditions of the present study, a daily dose of 300 mg of hydralazine was as effective and free of side effects given in 2 as in 4 divided doses."}
{"id": "PMID:1102236", "title": "Occurrence, transport, and storage of octopamine in human thrombocytes.", "content": "3H-octopamine was found to be accumulated in human platelets, achieving a maximum concentration gradient of 30:1. Its accumulation was partially inhibited by reserpine, imipramine, serotonin, ouabain, dinitrophenol, and iodoacetate. When octopamine was added to platelet preparations, it led to a decrease of both endogenous and 14C-serotonin. To determine whether octopamine accumulates in human platelets in vivo, preparations from 6 patients receiving monoamine oxidase inhibitors and 17 control subjects were assayed enzymatically for octopamine. Octopamine was detectable in all of the drug-treated patients, averaging 0.45 +/- 0.06 ng/mg protein, while only 4 of the 17 control subjects had detectable (greater than 0.05 ng/mg protein) platelet octopamine. Although much lower than platelet serotonin levels, these octopamine levels are in the range of those reported for platelet norepinephrine and epinephrine.", "contents": "Occurrence, transport, and storage of octopamine in human thrombocytes. 3H-octopamine was found to be accumulated in human platelets, achieving a maximum concentration gradient of 30:1. Its accumulation was partially inhibited by reserpine, imipramine, serotonin, ouabain, dinitrophenol, and iodoacetate. When octopamine was added to platelet preparations, it led to a decrease of both endogenous and 14C-serotonin. To determine whether octopamine accumulates in human platelets in vivo, preparations from 6 patients receiving monoamine oxidase inhibitors and 17 control subjects were assayed enzymatically for octopamine. Octopamine was detectable in all of the drug-treated patients, averaging 0.45 +/- 0.06 ng/mg protein, while only 4 of the 17 control subjects had detectable (greater than 0.05 ng/mg protein) platelet octopamine. Although much lower than platelet serotonin levels, these octopamine levels are in the range of those reported for platelet norepinephrine and epinephrine."}
{"id": "PMID:1102237", "title": "Clinical trials of drugs from the viewpoint of the academic investigator (a satire).", "content": "Dr. Lasagna was unable to attend the workshop in person, and therefore delivered his remarks by videotape, in the assumed role of the newest \"Secretary of Human Experimentation\" at the Department of Health, Education, and Welfare addressing a public television audience. There is no difficulty in sensing the serious truths underlying the satire in his address.", "contents": "Clinical trials of drugs from the viewpoint of the academic investigator (a satire). Dr. Lasagna was unable to attend the workshop in person, and therefore delivered his remarks by videotape, in the assumed role of the newest \"Secretary of Human Experimentation\" at the Department of Health, Education, and Welfare addressing a public television audience. There is no difficulty in sensing the serious truths underlying the satire in his address."}
{"id": "PMID:1102241", "title": "Panel 2: phase II investigations.", "content": "At the end of Phase II, a decision must be made as to whether or not the drug should be developed as a therapeutic agent. Such a decision may be based on many criteria in addition to the scientific data derived from the Phase II study. At this point, expert judgment is needed. One should like to assume that if the decision is made to proceed with the increasingly expensive and laborious process of further development, the drug will, barring some completely unforeseen misadventure, ultimately find its way into clinical therapeutics. Phase II studies, therefore, are the most crucial stage in the course of drug development. Planning of these studies requires great care and investigators of the highest caliber should be used for their conduct. The escalating costs of new drug development are resulting in an undesirable imbalance of efforts in the direction of studies characterized more by easily defined end points than by therapeutic needs. Industry, academia, and the FDA must all be concerned with facilitationg studies in areas of most-needed therapeutic advances.", "contents": "Panel 2: phase II investigations. At the end of Phase II, a decision must be made as to whether or not the drug should be developed as a therapeutic agent. Such a decision may be based on many criteria in addition to the scientific data derived from the Phase II study. At this point, expert judgment is needed. One should like to assume that if the decision is made to proceed with the increasingly expensive and laborious process of further development, the drug will, barring some completely unforeseen misadventure, ultimately find its way into clinical therapeutics. Phase II studies, therefore, are the most crucial stage in the course of drug development. Planning of these studies requires great care and investigators of the highest caliber should be used for their conduct. The escalating costs of new drug development are resulting in an undesirable imbalance of efforts in the direction of studies characterized more by easily defined end points than by therapeutic needs. Industry, academia, and the FDA must all be concerned with facilitationg studies in areas of most-needed therapeutic advances."}
{"id": "PMID:1102255", "title": "Imaging studies in renal failure. Emphasis of selection and sequencing in the clinical evaluation.", "content": "The focus of this review will be on the current role of imaging studies in the clinical evaluation of patients with acute and chronic renal failure. We will stress the application of these studies in specific clinical settings in an effort to indicate the current value, and also to suggest fruitful areas for future clinical investigation. It is clear that imaging studies are an essential component in the evaluation of acute and chronic renal failure. Physicians who manage such patients must choose appropriately from a variety of available studies to obtain a maximal information with the least risk to their patients. This selection is influenced by local conditions, the most important among these being the experience and technical skill of those involved. Attention to detail is particularly important and it is critical that the studies be done at the appropriate time in the evaluation. There will be emphasis on the evolving role of imaging studies in evaluating these patients.", "contents": "Imaging studies in renal failure. Emphasis of selection and sequencing in the clinical evaluation. The focus of this review will be on the current role of imaging studies in the clinical evaluation of patients with acute and chronic renal failure. We will stress the application of these studies in specific clinical settings in an effort to indicate the current value, and also to suggest fruitful areas for future clinical investigation. It is clear that imaging studies are an essential component in the evaluation of acute and chronic renal failure. Physicians who manage such patients must choose appropriately from a variety of available studies to obtain a maximal information with the least risk to their patients. This selection is influenced by local conditions, the most important among these being the experience and technical skill of those involved. Attention to detail is particularly important and it is critical that the studies be done at the appropriate time in the evaluation. There will be emphasis on the evolving role of imaging studies in evaluating these patients."}
{"id": "PMID:1102256", "title": "Occult traumatic lesions of the cervical vertebrae.", "content": "The numerous general and special techniques for examination of the cervical spine, with particular reference to the demonstration of occult traumatic lesions will be presented and evaluated. The advantages and limitations of each technique will be presented. Optimal procedures will be suggested for the examination of the acutely injured patient, with injuries of unknown severity, as well as for the longer term followups and minor injuries. The mechanisms of the production of cervical spine traumatic lesions and the long term abnormalities to be anticipated will be related, together with the types of radiographic findings associated with them. These will be presented in the context of the anatomy, function and dynamic physiology of the cervical spine. The dynamic interrelationship between segments of the cervical spine and the cervical spine as a whole, as well as the interrelation of the cervical spine with the skull and the remainder of the spine will be considered. The clinical data available in the literature will be collected and correlated with the radiographic findings. The results will be used to evaluate the clinical significance of these occult traumatic lesions, and the efficacy of therapy.", "contents": "Occult traumatic lesions of the cervical vertebrae. The numerous general and special techniques for examination of the cervical spine, with particular reference to the demonstration of occult traumatic lesions will be presented and evaluated. The advantages and limitations of each technique will be presented. Optimal procedures will be suggested for the examination of the acutely injured patient, with injuries of unknown severity, as well as for the longer term followups and minor injuries. The mechanisms of the production of cervical spine traumatic lesions and the long term abnormalities to be anticipated will be related, together with the types of radiographic findings associated with them. These will be presented in the context of the anatomy, function and dynamic physiology of the cervical spine. The dynamic interrelationship between segments of the cervical spine and the cervical spine as a whole, as well as the interrelation of the cervical spine with the skull and the remainder of the spine will be considered. The clinical data available in the literature will be collected and correlated with the radiographic findings. The results will be used to evaluate the clinical significance of these occult traumatic lesions, and the efficacy of therapy."}
{"id": "PMID:1102259", "title": "Clinical evaluation of two daily doses of naproxen and indomethacin: result of a double-blind crossover trail.", "content": "A double-blind crossover clinical trial is reported on the effects of naproxen in two doses, 500 mg. and 750 mg. daily, and 100 mg. indomethacin daily in 23 patients with classical rheumatoid arthritis, each drug being given for 1 week. The results show that there was little to choose between the drugs and that there was clinical equivalence between the two doses of naproxen. Radioactive pertechnetate (99mTc) joint uptakes were depressed by both naproxen and indomethacin, indicating anti-inflammatory effect.", "contents": "Clinical evaluation of two daily doses of naproxen and indomethacin: result of a double-blind crossover trail. A double-blind crossover clinical trial is reported on the effects of naproxen in two doses, 500 mg. and 750 mg. daily, and 100 mg. indomethacin daily in 23 patients with classical rheumatoid arthritis, each drug being given for 1 week. The results show that there was little to choose between the drugs and that there was clinical equivalence between the two doses of naproxen. Radioactive pertechnetate (99mTc) joint uptakes were depressed by both naproxen and indomethacin, indicating anti-inflammatory effect."}
{"id": "PMID:1102260", "title": "A double-blind trial of methocarbamol versus placebo in painful muscle spasm.", "content": "A double-blind trial of methocarbamol versus placebo was carried out in 59 matched pairs of patients suffering from painful muscle spasm. Methocarbamol (1500 mg. q.d.s.) was found to be effective in approximately 60% of patients compared with 30% of patients receiving placebo (p less than 0.01). Side-effects were of almost equal incidence in the two groups.", "contents": "A double-blind trial of methocarbamol versus placebo in painful muscle spasm. A double-blind trial of methocarbamol versus placebo was carried out in 59 matched pairs of patients suffering from painful muscle spasm. Methocarbamol (1500 mg. q.d.s.) was found to be effective in approximately 60% of patients compared with 30% of patients receiving placebo (p less than 0.01). Side-effects were of almost equal incidence in the two groups."}
{"id": "PMID:1102264", "title": "A preparatory technique for examination of imperfect fungi by scanning electron microscopy.", "content": "Thin 12 mm diameter coverslips, coated with nutrient agar, were inoculated with a fungus, incubated, and sequentially examined with the light microscope and then the scanning electron microscope (SEM), thus providing the valuable capability of correlation of results obtained from these two microscopic analyses. A sandwich of two coverslips was prepared for light-microscopic observations, and then separated and the agar-coated coverslip on which the fungus had grown was passed through fixative solutions, critical point dried, metal-coated and examined in the SEM. The technique was designed primarily for studies of conidiogenesis in rapidly growing human pathogenic imperfect fungi.", "contents": "A preparatory technique for examination of imperfect fungi by scanning electron microscopy. Thin 12 mm diameter coverslips, coated with nutrient agar, were inoculated with a fungus, incubated, and sequentially examined with the light microscope and then the scanning electron microscope (SEM), thus providing the valuable capability of correlation of results obtained from these two microscopic analyses. A sandwich of two coverslips was prepared for light-microscopic observations, and then separated and the agar-coated coverslip on which the fungus had grown was passed through fixative solutions, critical point dried, metal-coated and examined in the SEM. The technique was designed primarily for studies of conidiogenesis in rapidly growing human pathogenic imperfect fungi."}
{"id": "PMID:1102265", "title": "The possibility of latent centromeres and a proposed nomenclature system for total chromosome and whole arm translocations.", "content": "Translocations involving entire chromosomes or whole chromosome arms may not necessarily require deletion of a centromere. Conceivably, in the process of centromeric or telomeric fusion or of fusion of a centromere with a telomere, centromeric inactivation may occur, thus preserving both centromeres--one functional, the other latent--in the resultant translocation chromosome. If such latent centromeres exist and, in addition, are capable of being reactivated, it would explain how additional functional centromeres are acquired in the reverse process of chromosomal fission or fragmentation. A system of nomenclature is proposed for identifying the origin and nature of these chromosomal rearrangements.", "contents": "The possibility of latent centromeres and a proposed nomenclature system for total chromosome and whole arm translocations. Translocations involving entire chromosomes or whole chromosome arms may not necessarily require deletion of a centromere. Conceivably, in the process of centromeric or telomeric fusion or of fusion of a centromere with a telomere, centromeric inactivation may occur, thus preserving both centromeres--one functional, the other latent--in the resultant translocation chromosome. If such latent centromeres exist and, in addition, are capable of being reactivated, it would explain how additional functional centromeres are acquired in the reverse process of chromosomal fission or fragmentation. A system of nomenclature is proposed for identifying the origin and nature of these chromosomal rearrangements."}
{"id": "PMID:1102266", "title": "Potent combinations of beta-lactam antibiotics using the beta-lactamase inhibition principle.", "content": "Several penicillins known to be stable to enterobacterial beta-lactamases were tested in combination with beta-lactamase-sensitive penicillins and cephalosporins in a turbidimetric system. Nafcillin was found to be the best beta-lactamase inhibitor amongst agents presently available commercially, but the related, new semi-synthetic penicillin BRL 1437 (2-isopropoxy-1-naphthylpenicillin) was consistently found to be superior. Using 103 ampicillin-resistant coliform bacilli and antibiotic levels achievable in urine, cephalothin or cephaloridine alone achieved long-term suppression of growth (greater than 20 h) of 16 and 13% of strains, respectively, while the additional presence of BRL 1437 suppressed growth for longer than 20 h of 81% of the remaining strains. Even where 'success' was not achieved according to these stringent criteria, regrowth was significantly delayed by the presence of BRL 1437. Suppression of growth for longer than 20 h by BRL 1437 plus cephalothin was achieved with all of the 46 Escherichia coli strains tested. Antibiotic combinations were also studied in an in vitro model which stimulates the hydrokinetic features of the urinary bladder. Suppression of the growth of two highly resistant E. coli strains was achieved in this system, for therapeutically acceptable periods of time, with combinations of cephalothin or cephaloridine with BRL 1437, but not nafcillin.", "contents": "Potent combinations of beta-lactam antibiotics using the beta-lactamase inhibition principle. Several penicillins known to be stable to enterobacterial beta-lactamases were tested in combination with beta-lactamase-sensitive penicillins and cephalosporins in a turbidimetric system. Nafcillin was found to be the best beta-lactamase inhibitor amongst agents presently available commercially, but the related, new semi-synthetic penicillin BRL 1437 (2-isopropoxy-1-naphthylpenicillin) was consistently found to be superior. Using 103 ampicillin-resistant coliform bacilli and antibiotic levels achievable in urine, cephalothin or cephaloridine alone achieved long-term suppression of growth (greater than 20 h) of 16 and 13% of strains, respectively, while the additional presence of BRL 1437 suppressed growth for longer than 20 h of 81% of the remaining strains. Even where 'success' was not achieved according to these stringent criteria, regrowth was significantly delayed by the presence of BRL 1437. Suppression of growth for longer than 20 h by BRL 1437 plus cephalothin was achieved with all of the 46 Escherichia coli strains tested. Antibiotic combinations were also studied in an in vitro model which stimulates the hydrokinetic features of the urinary bladder. Suppression of the growth of two highly resistant E. coli strains was achieved in this system, for therapeutically acceptable periods of time, with combinations of cephalothin or cephaloridine with BRL 1437, but not nafcillin."}
{"id": "PMID:1102267", "title": "Adjuvant immunotherapy for malignant brain tumors in infants and children.", "content": "Immune deficiency of immunocompetent cells or of humoral factors are essential causes of tumor growth. The authors have investigated the transfer of immunocompetent cells - allogeneic bone marrow cell transfusion and white blood cell intracranial infusion - for the treatment of 11 malignant gliomas in infants and children as an adjuvant to surgery, radiation and/or chemotherapy. Ten cases, from 3 months to 11 years, received bone marrow cell transfusion. Two medulloblastomas and 3 pontine gliomas are dead. Five cases are alive and well 37-65 months following surgery. Among these two posterior fossa neoplasms, a medulloblastoma and a glioblastoma have survived 46 and 65 months, respectively. One cerebral glioblastoma received allogeneic white blood cells infused locally into the tumor bed: it recurred 1 year following surgery, chemotherapy, and immunotherapy. Cytolysis of the tumor cells by sensitized lymphoid cells were demonstrated in this case. The role of immunotherapy should be limited at the present time to adjuvant therapy until its effect on tumor growth is statistically confirmed. The results so far are promising, and improvement of the immunological approach in treating malignant brain tumors is under way.", "contents": "Adjuvant immunotherapy for malignant brain tumors in infants and children. Immune deficiency of immunocompetent cells or of humoral factors are essential causes of tumor growth. The authors have investigated the transfer of immunocompetent cells - allogeneic bone marrow cell transfusion and white blood cell intracranial infusion - for the treatment of 11 malignant gliomas in infants and children as an adjuvant to surgery, radiation and/or chemotherapy. Ten cases, from 3 months to 11 years, received bone marrow cell transfusion. Two medulloblastomas and 3 pontine gliomas are dead. Five cases are alive and well 37-65 months following surgery. Among these two posterior fossa neoplasms, a medulloblastoma and a glioblastoma have survived 46 and 65 months, respectively. One cerebral glioblastoma received allogeneic white blood cells infused locally into the tumor bed: it recurred 1 year following surgery, chemotherapy, and immunotherapy. Cytolysis of the tumor cells by sensitized lymphoid cells were demonstrated in this case. The role of immunotherapy should be limited at the present time to adjuvant therapy until its effect on tumor growth is statistically confirmed. The results so far are promising, and improvement of the immunological approach in treating malignant brain tumors is under way."}
{"id": "PMID:1102268", "title": "Manifestations and management of Arnold-Chiari malformation in patients with myelomeningocele.", "content": "As a result of the authors' experience they advocate posterior fossa decompression in patients with myelomeningocele at the first sign of compression of bratn stem or the cervical cord or both. If decompression is not undertaken at once the impaired gag and cough reflexes place the child at risk. He may develop pneumonia. If decompression is delayed too long full neurological recovery will not occur. The removal of bone and the opening of dura must be carried down to the bottom of the tonsillar tip, sometimes as low as C7. The most dramatic improvement occurs in infants whose symptoms are life threatening. Less dramatic but just as real is the improvement in older children. Although investigations such as myelography and ventriculography will show the malformation, they are not essential. The associated visible malformations and the clinical condition are the most reliable means of making the diagnosis. Delaying the decompression risks the life of the infant and threatens the quality of life for the older child.", "contents": "Manifestations and management of Arnold-Chiari malformation in patients with myelomeningocele. As a result of the authors' experience they advocate posterior fossa decompression in patients with myelomeningocele at the first sign of compression of bratn stem or the cervical cord or both. If decompression is not undertaken at once the impaired gag and cough reflexes place the child at risk. He may develop pneumonia. If decompression is delayed too long full neurological recovery will not occur. The removal of bone and the opening of dura must be carried down to the bottom of the tonsillar tip, sometimes as low as C7. The most dramatic improvement occurs in infants whose symptoms are life threatening. Less dramatic but just as real is the improvement in older children. Although investigations such as myelography and ventriculography will show the malformation, they are not essential. The associated visible malformations and the clinical condition are the most reliable means of making the diagnosis. Delaying the decompression risks the life of the infant and threatens the quality of life for the older child."}
{"id": "PMID:1102270", "title": "[Hyperacute kidney graft rejection with and without demonstration of antibodies].", "content": "Hyperacute rejection was studied in presensitized recipients of renal homografts and pig-to-dog heterografts. In these experiments antidonor antibodies can be observed but their participation in the rejection process cannot be assessed quantitatively. The importance of antibodies lies in their capacity to activate the complement system, which in turn destroys the graft. Intrarenal complement activation was also demonstrated in two clinical cases of hyperacute rejection, in one of which activation was by the alternate pathway.", "contents": "[Hyperacute kidney graft rejection with and without demonstration of antibodies]. Hyperacute rejection was studied in presensitized recipients of renal homografts and pig-to-dog heterografts. In these experiments antidonor antibodies can be observed but their participation in the rejection process cannot be assessed quantitatively. The importance of antibodies lies in their capacity to activate the complement system, which in turn destroys the graft. Intrarenal complement activation was also demonstrated in two clinical cases of hyperacute rejection, in one of which activation was by the alternate pathway."}
{"id": "PMID:1102271", "title": "[Aspergillus infection in skin transplantation and its therapy].", "content": "In a 10 years old girl sustaining a corrosive injury of the lower leg from sulphuric acid, in the region of a skin transplantation colonization with Aspergillus fumigatus (Fresenium) and Aspergillus niger (van Tieghem) took place. This infection endangered the attempt of transplantation and the saving of the foot. Treatment by medication with nystatin (moronal) and canesten (clotrimazol) were ineffective. Pimaricin (pimafucin, natamycin) quickly erradicated the mycotic infection and secured an undisturbed progress for the transplantation. Additionally the epidemiology of infections by Aspergillus is briefly discussed.", "contents": "[Aspergillus infection in skin transplantation and its therapy]. In a 10 years old girl sustaining a corrosive injury of the lower leg from sulphuric acid, in the region of a skin transplantation colonization with Aspergillus fumigatus (Fresenium) and Aspergillus niger (van Tieghem) took place. This infection endangered the attempt of transplantation and the saving of the foot. Treatment by medication with nystatin (moronal) and canesten (clotrimazol) were ineffective. Pimaricin (pimafucin, natamycin) quickly erradicated the mycotic infection and secured an undisturbed progress for the transplantation. Additionally the epidemiology of infections by Aspergillus is briefly discussed."}
{"id": "PMID:1102277", "title": "The assessment of thiothixene in chronic schizophrenia. A double-blind controlled trial.", "content": "The results are reported of a double-blind controlled trial of a recently introduced major tranquilizer thiothixene (Navane, Pfizer) against chlorpromazine in 24 chronic schizophrenics in the Royal Edinburgh Hospital, Edinburgh, Scotland. Additionally, a limited experiment in withdrawal of routine tranquilizers in these patients (one month) was carried out prior to the trial. Changes in manifest psychosis (symptoms) and social disability were assessed by the use of the Lorr scale (IMPS) and the Nurses' Observation Scale for Inpatient Evaluation (NOSIE) at suitable intervals. The results indicated that thiothixene conferred no advantage over chlorpromazine in symptom relief or social improvement. Serial measures showed no significant change in these patients as a group when on active drugs as opposed to placebo, or with the course of time. 12.5% of patients relapsed during the placebo period: these patients were younger than the non-relapsers and had received larger daily doses of phenothiazines prior to the trial.", "contents": "The assessment of thiothixene in chronic schizophrenia. A double-blind controlled trial. The results are reported of a double-blind controlled trial of a recently introduced major tranquilizer thiothixene (Navane, Pfizer) against chlorpromazine in 24 chronic schizophrenics in the Royal Edinburgh Hospital, Edinburgh, Scotland. Additionally, a limited experiment in withdrawal of routine tranquilizers in these patients (one month) was carried out prior to the trial. Changes in manifest psychosis (symptoms) and social disability were assessed by the use of the Lorr scale (IMPS) and the Nurses' Observation Scale for Inpatient Evaluation (NOSIE) at suitable intervals. The results indicated that thiothixene conferred no advantage over chlorpromazine in symptom relief or social improvement. Serial measures showed no significant change in these patients as a group when on active drugs as opposed to placebo, or with the course of time. 12.5% of patients relapsed during the placebo period: these patients were younger than the non-relapsers and had received larger daily doses of phenothiazines prior to the trial."}
{"id": "PMID:1102282", "title": "[Contaminated infusions as cause of nosocomial Serratia marcescens septicaemia in children (author's transl)].", "content": "At the University Children's Clinic at Munich 25 cases of Serratia marescens septicaemia (mainly bacteriocin types 18 and 44) occurred within one year, predominantly in newborns and infants. Almost all of the children were seriously ill from an underlying illness or had been operated on. Two thirds had received antibiotics before the septicaemia occurred but they were ineffective. Of a total of nine antibiotics tested against 51 Serratia marcescens strains, nalidixic acid (99% sensitivity) and amikacin (100%) proved the most effective. Main source of the septicaemia were contaminated infusions, from which in as many as 35% of cases microorganisms, usually Serratia marcescens, had been isolated. Intensive hygienic measures at once terminated this \"sepsis wave\".", "contents": "[Contaminated infusions as cause of nosocomial Serratia marcescens septicaemia in children (author's transl)]. At the University Children's Clinic at Munich 25 cases of Serratia marescens septicaemia (mainly bacteriocin types 18 and 44) occurred within one year, predominantly in newborns and infants. Almost all of the children were seriously ill from an underlying illness or had been operated on. Two thirds had received antibiotics before the septicaemia occurred but they were ineffective. Of a total of nine antibiotics tested against 51 Serratia marcescens strains, nalidixic acid (99% sensitivity) and amikacin (100%) proved the most effective. Main source of the septicaemia were contaminated infusions, from which in as many as 35% of cases microorganisms, usually Serratia marcescens, had been isolated. Intensive hygienic measures at once terminated this \"sepsis wave\"."}
{"id": "PMID:1102295", "title": "Stimulation of LH release and ovulation in the rat by cutaneous application of luteinizing hormone-releasing hormone (LH-RH) in dimethyl sulfoxide (DMSO).", "content": "The efficacy of cutaneously applied luteinizing hormone releasing hormone (LH-RH)  in stimulating LH release in the chronically ovariectomized, estrogen/progesterone-blocked rat and ovulation in the chlorpromazine-blocked, proestrous rat was investigated. Following the cutaneous application of 25 or 100 mug of LH-RH in 100% dimethyl sulfoxide (DMSO), serum LH rose to a peak at 1 hr, then declined toward basal levels in the ensuing 3 to 4 hr. LH-RH applied cutaneously in either 100% DMSO or 0.9% NaCl was also capable of inducing ovulation in the chlorpromazine-blocked, proestrous rat; however, LH-RH in 0.9% NaCl did not yield a linear log-dose-response relationship, therefore a valid ED50 potency ratio for DMSO/0.9% NaCl could not be estimated. Nonetheless, 0.9% NaCl was a considerably less effective cutaneous vehicle than DMSO. Cutaneous application of LH-RH in DMSO in doses of 100, 50, 25, 10, 5, 2.5 and 1 mug induced ovulation in 100%, 100%, 90%, 73%, 30%, 10% and 0% of the rats, respectively. When the percent ovulation response was transformed to probits and plotted against the logarithm of the LH-RH dose an approximately linear log dose-response was obtained; the same relationship also held true for ovulation induction following sc LH-RH administration. In turn, the ED50's of these two parallel dose-response curves yielded a potency ratio estimate (sc/cutaneous) of 0.025, suggesting that 2.5% of the cutaneously applied LH-RH dose was absorbed through the skin in a biologically active form. These data indicate that DMSO privdes a convenient cutaneous vehicle for the administration of LH-RH.", "contents": "Stimulation of LH release and ovulation in the rat by cutaneous application of luteinizing hormone-releasing hormone (LH-RH) in dimethyl sulfoxide (DMSO). The efficacy of cutaneously applied luteinizing hormone releasing hormone (LH-RH)  in stimulating LH release in the chronically ovariectomized, estrogen/progesterone-blocked rat and ovulation in the chlorpromazine-blocked, proestrous rat was investigated. Following the cutaneous application of 25 or 100 mug of LH-RH in 100% dimethyl sulfoxide (DMSO), serum LH rose to a peak at 1 hr, then declined toward basal levels in the ensuing 3 to 4 hr. LH-RH applied cutaneously in either 100% DMSO or 0.9% NaCl was also capable of inducing ovulation in the chlorpromazine-blocked, proestrous rat; however, LH-RH in 0.9% NaCl did not yield a linear log-dose-response relationship, therefore a valid ED50 potency ratio for DMSO/0.9% NaCl could not be estimated. Nonetheless, 0.9% NaCl was a considerably less effective cutaneous vehicle than DMSO. Cutaneous application of LH-RH in DMSO in doses of 100, 50, 25, 10, 5, 2.5 and 1 mug induced ovulation in 100%, 100%, 90%, 73%, 30%, 10% and 0% of the rats, respectively. When the percent ovulation response was transformed to probits and plotted against the logarithm of the LH-RH dose an approximately linear log dose-response was obtained; the same relationship also held true for ovulation induction following sc LH-RH administration. In turn, the ED50's of these two parallel dose-response curves yielded a potency ratio estimate (sc/cutaneous) of 0.025, suggesting that 2.5% of the cutaneously applied LH-RH dose was absorbed through the skin in a biologically active form. These data indicate that DMSO privdes a convenient cutaneous vehicle for the administration of LH-RH."}
{"id": "PMID:1102296", "title": "Anti-insulin serum coupled to Sepharose 4B as a tool for the investigation of insulin biosynthesis in the B-cells of obese hyperglycemic mice.", "content": "The biosynthetic activity of the B-cells of obese hyperglycemic mice (obob) was measured by the incorporation of [3H]leucine into proteins in collagenase-isolated pancreatic islets. To quantitate the incorporation into proinsulin and insulin, an immune binding method was used. For this purpose, anti-insulin serum was coupled to cyanogen bromide-activated SepharoseR 4B. This turned out to be a specific and versatile technique for the measurement of newly synthesized proinsulin and insulin in the B-cells. The B-cells of obob mice appear to be well adapted to a high rate of hormone biosynthesis, since at 16.7 mM glucose 44% of [3H]leucine incorporated into TCA-precipitable proteins was bound to the insulin antibodies coupled to Sepharose 4B. The insulin biosynthetic rate was stimulated 9 times at 16.7 mM glucose, during a 3-h incubation, compared with the basal insulin biosynthesis rate.", "contents": "Anti-insulin serum coupled to Sepharose 4B as a tool for the investigation of insulin biosynthesis in the B-cells of obese hyperglycemic mice. The biosynthetic activity of the B-cells of obese hyperglycemic mice (obob) was measured by the incorporation of [3H]leucine into proteins in collagenase-isolated pancreatic islets. To quantitate the incorporation into proinsulin and insulin, an immune binding method was used. For this purpose, anti-insulin serum was coupled to cyanogen bromide-activated SepharoseR 4B. This turned out to be a specific and versatile technique for the measurement of newly synthesized proinsulin and insulin in the B-cells. The B-cells of obob mice appear to be well adapted to a high rate of hormone biosynthesis, since at 16.7 mM glucose 44% of [3H]leucine incorporated into TCA-precipitable proteins was bound to the insulin antibodies coupled to Sepharose 4B. The insulin biosynthetic rate was stimulated 9 times at 16.7 mM glucose, during a 3-h incubation, compared with the basal insulin biosynthesis rate."}
{"id": "PMID:1102297", "title": "Blockage of estrogen-induced release of luteinizing hormone by reserpine and potentiation of synthetic gonadotropin-releasing hormone-induced release of luteinizing hormone by estrogen in the ovariectomized ewe.", "content": "These experiments tested the effects of reserpine on estrogen-induced LH release and the effects of estrogen on gonadotropin-releasing hormone-induced LH release in ovariectomized ewes. Injection of 50, 200, or 500 mug of estradiol benzoate (EB) into progesterone-treated ewes, or of 50 mug of EB into non-treated ewes induced a large surge in serum LH levels approximately 15 h later. Injection of 5 mg/ewe of reserpine 6 h prior to the EB, blocked the LH surge. Reserpine reduced, but did not block the release of LH induced by injection of 20 mug or 40 mug of synthetic gonadotropin-releasing hormone (GnRH). Injection of graded doses (5, 10, or 20 mug) of GnRH into progesterone-treated ewes that had been treated with reserpine, induced increases in serum LH levels that were proportional to the logarithm of the dose. In the final series of experiments both progesterone-treated and non-treated ewes were injected with reserpine and then with either EB or oil. Fifteen hours after receiving EB or oil they were injected with either GnRH or the diluent. GnRH elevated serum LH levels significantly higher in ewes given EB than in those given oil. LH levels in ewes given oil and diluent were not elevated. These data suggest that reserpine blocked EB-induced LH release by an action on the central nervous system and that EB acted directly on the anterior pituitary to increase the response to GnRH.", "contents": "Blockage of estrogen-induced release of luteinizing hormone by reserpine and potentiation of synthetic gonadotropin-releasing hormone-induced release of luteinizing hormone by estrogen in the ovariectomized ewe. These experiments tested the effects of reserpine on estrogen-induced LH release and the effects of estrogen on gonadotropin-releasing hormone-induced LH release in ovariectomized ewes. Injection of 50, 200, or 500 mug of estradiol benzoate (EB) into progesterone-treated ewes, or of 50 mug of EB into non-treated ewes induced a large surge in serum LH levels approximately 15 h later. Injection of 5 mg/ewe of reserpine 6 h prior to the EB, blocked the LH surge. Reserpine reduced, but did not block the release of LH induced by injection of 20 mug or 40 mug of synthetic gonadotropin-releasing hormone (GnRH). Injection of graded doses (5, 10, or 20 mug) of GnRH into progesterone-treated ewes that had been treated with reserpine, induced increases in serum LH levels that were proportional to the logarithm of the dose. In the final series of experiments both progesterone-treated and non-treated ewes were injected with reserpine and then with either EB or oil. Fifteen hours after receiving EB or oil they were injected with either GnRH or the diluent. GnRH elevated serum LH levels significantly higher in ewes given EB than in those given oil. LH levels in ewes given oil and diluent were not elevated. These data suggest that reserpine blocked EB-induced LH release by an action on the central nervous system and that EB acted directly on the anterior pituitary to increase the response to GnRH."}
{"id": "PMID:1102299", "title": "Modulation of the enzymic activity of chicken pepsin by the covalent modification of its single -- SH group.", "content": "The single cysteinyl residue of chicken pepsin was modified with a wide spectrum of reagents to produce mixed disulfides or alkylated derivatives. All these derivatives showed enhanced catalytic activity towards the synthetic peptide Z-Ala-Ala-Phe-OPrP, where OPrP is the 3-(4-pyridyl)-propyl-1-oxy group. The overall catalytic constant kcat/Km for these derivatives was 4 -- 25-fold larger than that of the native enzyme. The activity of the enzyme towards denatured hemoglobin was slightly decreased (10--45%) by these modifications. When the mixed disulfide derivatives were treated with excess mercaptan, the sulfhydryl group was regenerated and activity reverted to that of the native enzyme. The --SH group of chicken pepsin reacted preferentially with reagents containing an aromatic group. The reaction was found to depend on the ionization of a single group, presumably the --SH itself, with a pKa = 7.5. The rate of reaction of the fully deprotonated species with various disulfides was 100--1000-fold smaller than that of the --SH group of glutathione. It is suggested that the groups attached covalently to  the sulfhydryl also interact with other amino acid side chains in the protein thereby affecting the active center of chicken pepsin.", "contents": "Modulation of the enzymic activity of chicken pepsin by the covalent modification of its single -- SH group. The single cysteinyl residue of chicken pepsin was modified with a wide spectrum of reagents to produce mixed disulfides or alkylated derivatives. All these derivatives showed enhanced catalytic activity towards the synthetic peptide Z-Ala-Ala-Phe-OPrP, where OPrP is the 3-(4-pyridyl)-propyl-1-oxy group. The overall catalytic constant kcat/Km for these derivatives was 4 -- 25-fold larger than that of the native enzyme. The activity of the enzyme towards denatured hemoglobin was slightly decreased (10--45%) by these modifications. When the mixed disulfide derivatives were treated with excess mercaptan, the sulfhydryl group was regenerated and activity reverted to that of the native enzyme. The --SH group of chicken pepsin reacted preferentially with reagents containing an aromatic group. The reaction was found to depend on the ionization of a single group, presumably the --SH itself, with a pKa = 7.5. The rate of reaction of the fully deprotonated species with various disulfides was 100--1000-fold smaller than that of the --SH group of glutathione. It is suggested that the groups attached covalently to  the sulfhydryl also interact with other amino acid side chains in the protein thereby affecting the active center of chicken pepsin."}
{"id": "PMID:1102300", "title": "Initiation specificity of the poly(cytidylic acid)-dependent Qbeta replicase activity.", "content": "The initiation specificity of RNA synthesis catalysed by the poly(C)-dependent Qbeta replicase activity was investigated with various synthetic ribopolymers as template. It was found that the initiation efficiency of a series of oligo(C) with various chain lengths is proportional to the template size. Synthetic riboheteropolymers containing cytidylic acid were accepted as templates only if they contained at their 3' end a cytidylic acid sequence of more than 5 nucleotides. Such an oligocytidylate sequence served also as an initiator sequence for copying the non-cytidylic-acid-containing part of the heterotemplate. RNA synthesis always began with the incorporation of GTP, even if the 3'-terminating nucleotide of the template was not cytidylic acid.", "contents": "Initiation specificity of the poly(cytidylic acid)-dependent Qbeta replicase activity. The initiation specificity of RNA synthesis catalysed by the poly(C)-dependent Qbeta replicase activity was investigated with various synthetic ribopolymers as template. It was found that the initiation efficiency of a series of oligo(C) with various chain lengths is proportional to the template size. Synthetic riboheteropolymers containing cytidylic acid were accepted as templates only if they contained at their 3' end a cytidylic acid sequence of more than 5 nucleotides. Such an oligocytidylate sequence served also as an initiator sequence for copying the non-cytidylic-acid-containing part of the heterotemplate. RNA synthesis always began with the incorporation of GTP, even if the 3'-terminating nucleotide of the template was not cytidylic acid."}
{"id": "PMID:1102301", "title": "Solution conformation of virginiamycins (staphylomycins).", "content": "The 1H (at 300 MHz) and 13C nuclear magnetic resonance spectra of virginiamycins S and S4 and vernamycin Balpha have been unravelled and analyzed. Together with model building and theoretical considerations, this allows the detailed description of their solution conformations. The depside bond can rotate and gives to the backbone some conformational mobility. The orientation of the depsicarbonyl bond depends on the surrounding. Apparent discrepancies between the different methods that are applicable for the disclosure of the nature of peptide H-bonding, have found a rational explanation.", "contents": "Solution conformation of virginiamycins (staphylomycins). The 1H (at 300 MHz) and 13C nuclear magnetic resonance spectra of virginiamycins S and S4 and vernamycin Balpha have been unravelled and analyzed. Together with model building and theoretical considerations, this allows the detailed description of their solution conformations. The depside bond can rotate and gives to the backbone some conformational mobility. The orientation of the depsicarbonyl bond depends on the surrounding. Apparent discrepancies between the different methods that are applicable for the disclosure of the nature of peptide H-bonding, have found a rational explanation."}
{"id": "PMID:1102302", "title": "On the dissociation and association of Escherichia coli ribosomes.", "content": "The dissociation and association behaviour of 70-S ribosomes of Escherichia coli has been studied. It has been shown that the dissociation-association reaction can be both a real dynamic equilibrium and a non-equilibrium reaction, dependent upon the ionic conditions of the solvent. At relatively high ionic strength (I = 0.15 M or more) the dissociation-association reaction is an equilibrium reaction, whereas at lower ionic strength (I = 0.1 M or less) there is no dynamic equilibrium between 70-S ribosomes and its subunits. In the latter case a hysteresis in the dissociation-association reaction is observed. Whether there is a dynamic equilibrium or not can be demonstrated by a single centrifugation experiment, using the analytical ultracentrifuge.", "contents": "On the dissociation and association of Escherichia coli ribosomes. The dissociation and association behaviour of 70-S ribosomes of Escherichia coli has been studied. It has been shown that the dissociation-association reaction can be both a real dynamic equilibrium and a non-equilibrium reaction, dependent upon the ionic conditions of the solvent. At relatively high ionic strength (I = 0.15 M or more) the dissociation-association reaction is an equilibrium reaction, whereas at lower ionic strength (I = 0.1 M or less) there is no dynamic equilibrium between 70-S ribosomes and its subunits. In the latter case a hysteresis in the dissociation-association reaction is observed. Whether there is a dynamic equilibrium or not can be demonstrated by a single centrifugation experiment, using the analytical ultracentrifuge."}
{"id": "PMID:1102303", "title": "Ultrastructural organization and biochemical characterization of chromatin - RNA - protein complexes isolated from mammalian cell nuclei.", "content": "Extranucleolar elements were isolated from mammalian cell nuclei and characterized by correlative biochemical-ultrastructural studies. These complex structures were shown to retain a supra-particle arrangement closely resembling the organization in situ of definite nuclear areas. In particular the morphological RNA - protein species, namely perichromatin fibrils and granules, were detected in association with characteristic regions of chromatin. By autoradiographic experiments, we were able to show that perichromatin fibrils represent the morphological state of newly formed heterogeneous nuclear RNA (hnRNA). Chromatin - RNA - protein complexes were further fractionated by means of a treatment dissociating the chromatin component. A minor part of DNA, possibly involved in the biosynthesis and the processing of hnRNA, remains linked to the resulting RNA - protein network.", "contents": "Ultrastructural organization and biochemical characterization of chromatin - RNA - protein complexes isolated from mammalian cell nuclei. Extranucleolar elements were isolated from mammalian cell nuclei and characterized by correlative biochemical-ultrastructural studies. These complex structures were shown to retain a supra-particle arrangement closely resembling the organization in situ of definite nuclear areas. In particular the morphological RNA - protein species, namely perichromatin fibrils and granules, were detected in association with characteristic regions of chromatin. By autoradiographic experiments, we were able to show that perichromatin fibrils represent the morphological state of newly formed heterogeneous nuclear RNA (hnRNA). Chromatin - RNA - protein complexes were further fractionated by means of a treatment dissociating the chromatin component. A minor part of DNA, possibly involved in the biosynthesis and the processing of hnRNA, remains linked to the resulting RNA - protein network."}
{"id": "PMID:1102304", "title": "Phage Qbeta replicase: cell-free synthesis of the phage-specific subunit and its assembly with host subunits to form active enzyme.", "content": "Cell-free translation of Qbeta RNA and subsequent partial purification of the enzyme resulted in replicase activity. From 0.5 to 1.5% of all R chains synthesised were found in the 7-S replicase complex. The presence in the 7-S complex of the host subunits of authentic replicase, i (= S1) and EF-Ts, was shown by the effect of antisera directed against ribosomal protein S1 and EF-Ts, respectively. Furthermore, the presence of EF-Ts was demonstrated by thermal denaturation of in vitro replicase made by a cell extract from an Escherichia coli mutant with a thermolabile EF-Ts. In vitro replicase did not assemble spontaneously during protein synthesis but was formed upon subsequent purification. Assembly could be induced by ammonium sulphate precipitation (60% saturation) alone. It is concluded that the functional phage-coded subunit synthesised in vitro recognises i and the EF-Tu - EF-Ts complex among a mixture of host proteins.", "contents": "Phage Qbeta replicase: cell-free synthesis of the phage-specific subunit and its assembly with host subunits to form active enzyme. Cell-free translation of Qbeta RNA and subsequent partial purification of the enzyme resulted in replicase activity. From 0.5 to 1.5% of all R chains synthesised were found in the 7-S replicase complex. The presence in the 7-S complex of the host subunits of authentic replicase, i (= S1) and EF-Ts, was shown by the effect of antisera directed against ribosomal protein S1 and EF-Ts, respectively. Furthermore, the presence of EF-Ts was demonstrated by thermal denaturation of in vitro replicase made by a cell extract from an Escherichia coli mutant with a thermolabile EF-Ts. In vitro replicase did not assemble spontaneously during protein synthesis but was formed upon subsequent purification. Assembly could be induced by ammonium sulphate precipitation (60% saturation) alone. It is concluded that the functional phage-coded subunit synthesised in vitro recognises i and the EF-Tu - EF-Ts complex among a mixture of host proteins."}
{"id": "PMID:1102305", "title": "The use of primed synthesis by DNA polymerase I to study an intercistronic sequence of phiX-174 DNA.", "content": "A decadeoxynucleotide complementary to ten nucleotides in the major ribosome-protected fragment of phiX-174 plus-strand DNA has been chemically synthesized and used as a primer for DNA polymerase I on phiX-174 plus-strand DNA as template. The sequence of the first 40 nucleotides incorporated onto the decadeoxynucleotide has been determined. This sequence extends further the sequence of the intercistronic region preceding gene G and shows the presence of another termination codon. The sequence was determined by using manganese as the activating cation for DNA polymerase I which allows ribonucleotides to be incorporated as well as deoxyribonucleotides. The ribo-substituted product was then cleaved specifically at the ribonucleotide residues to generate a series of overlapping ribo-terminated fragments whose sequences were sufficient to determine the complete sequence of the first 40 nucleotides. No evidence for misincorporation by DNA polymerase I in the presence of manganese was detected.", "contents": "The use of primed synthesis by DNA polymerase I to study an intercistronic sequence of phiX-174 DNA. A decadeoxynucleotide complementary to ten nucleotides in the major ribosome-protected fragment of phiX-174 plus-strand DNA has been chemically synthesized and used as a primer for DNA polymerase I on phiX-174 plus-strand DNA as template. The sequence of the first 40 nucleotides incorporated onto the decadeoxynucleotide has been determined. This sequence extends further the sequence of the intercistronic region preceding gene G and shows the presence of another termination codon. The sequence was determined by using manganese as the activating cation for DNA polymerase I which allows ribonucleotides to be incorporated as well as deoxyribonucleotides. The ribo-substituted product was then cleaved specifically at the ribonucleotide residues to generate a series of overlapping ribo-terminated fragments whose sequences were sufficient to determine the complete sequence of the first 40 nucleotides. No evidence for misincorporation by DNA polymerase I in the presence of manganese was detected."}
{"id": "PMID:1102306", "title": "The importance of Escherichia coli ribosomal proteins L1, L11 and L16 for the association of ribosomal subunits and the formation of the 70-S initiation complex.", "content": "50-S subunits were washed with LiCl solutions of different concentrations. After washing with 1 M LiCl solution the particles lost their ability to attach either to 30-S subunits or to the AUG - 30-S subunit - fMet-tRNAfMet complex or to a poly(U) - 30-S subunit - Phe-tRNAPhe complex. Those proteins which were removed by LiCl were fractionated on a Sephadex G-100 column. Of the fractionated proteins only the combinations L1 and L11 or L1 and L16 were essential for the association of 50-S 1.0 cores (particles prepared by washing 50-S subunits in 1.0 M LiCl) with 30-S subunits. These three proteins were also required for the formation of a stable complex between 50-S 1.0 cores, mRNA, 30-S subunits and aminoacyl-tRNA.", "contents": "The importance of Escherichia coli ribosomal proteins L1, L11 and L16 for the association of ribosomal subunits and the formation of the 70-S initiation complex. 50-S subunits were washed with LiCl solutions of different concentrations. After washing with 1 M LiCl solution the particles lost their ability to attach either to 30-S subunits or to the AUG - 30-S subunit - fMet-tRNAfMet complex or to a poly(U) - 30-S subunit - Phe-tRNAPhe complex. Those proteins which were removed by LiCl were fractionated on a Sephadex G-100 column. Of the fractionated proteins only the combinations L1 and L11 or L1 and L16 were essential for the association of 50-S 1.0 cores (particles prepared by washing 50-S subunits in 1.0 M LiCl) with 30-S subunits. These three proteins were also required for the formation of a stable complex between 50-S 1.0 cores, mRNA, 30-S subunits and aminoacyl-tRNA."}
{"id": "PMID:1102307", "title": "Interaction between arginase and L-ornithine carbamoyltransferase in Saccharomyces cerevisiae. The regulatory sites of arginase.", "content": "The inhibition of ornithine carbamoyltransferase by arginase in Saccharomyces cerevisiae, which is under the control or arginine and ornithine, involves a regulatory site for arginine on the arginase distinct from its catalytic site. This regulatory site is responsible for the reinforcement effect of arginine on the inhibition of ornithine carbamoyltransferase by arginase. The binding site of ornithine carbamoyltransferase on arginase is also shown by our analysis.", "contents": "Interaction between arginase and L-ornithine carbamoyltransferase in Saccharomyces cerevisiae. The regulatory sites of arginase. The inhibition of ornithine carbamoyltransferase by arginase in Saccharomyces cerevisiae, which is under the control or arginine and ornithine, involves a regulatory site for arginine on the arginase distinct from its catalytic site. This regulatory site is responsible for the reinforcement effect of arginine on the inhibition of ornithine carbamoyltransferase by arginase. The binding site of ornithine carbamoyltransferase on arginase is also shown by our analysis."}
{"id": "PMID:1102308", "title": "Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme.", "content": "N-Acetylmuramyl-L-alanine amidase activity was detected in Escherichia coli K 12 by usine N-acetylmuramyl-L-alanyl-gamma-D-glutamyl-(L)-meso-[3H]diaminopimelic acid as a radioactive substrate. This activity cleaves the amide bond between the residues of N-acetylmuramyl acid and L-alanine. It was readily obtained in a soluble form either by mechanical disruption of the cells or by spheroplast formation. In the latter case the release of most of the activity into the sucrose medium seems to indicate that it is either periplasmic or associated with the outer membrane of the envelope of E. coli K 12. The enzyme was purified to near homogeneity. A molecular weight of 39 000 was determined by gel filtration and confirmed by polyacrylamide gel electrophoresis. Further characterisation of this N-acetylmuramyl-L-alanine amidase activity was carried out by investigating several of its properties.", "contents": "Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme. N-Acetylmuramyl-L-alanine amidase activity was detected in Escherichia coli K 12 by usine N-acetylmuramyl-L-alanyl-gamma-D-glutamyl-(L)-meso-[3H]diaminopimelic acid as a radioactive substrate. This activity cleaves the amide bond between the residues of N-acetylmuramyl acid and L-alanine. It was readily obtained in a soluble form either by mechanical disruption of the cells or by spheroplast formation. In the latter case the release of most of the activity into the sucrose medium seems to indicate that it is either periplasmic or associated with the outer membrane of the envelope of E. coli K 12. The enzyme was purified to near homogeneity. A molecular weight of 39 000 was determined by gel filtration and confirmed by polyacrylamide gel electrophoresis. Further characterisation of this N-acetylmuramyl-L-alanine amidase activity was carried out by investigating several of its properties."}
{"id": "PMID:1102309", "title": "The isolation of two different lipopolysaccharide fractions from various Proteus mirabilis strains.", "content": "Four distinct Proteus mirabilis strains were extracted by the phenol/water procedure. After ultracentrifugation of the dialyzed water phase, the pelleted lipopolysaccharide was purified and analyzed. The sugar composition of this lipopolysaccharide fraction I was similar for all four strains, containing only small amounts of strain-specific constituents. A second lipopolysaccharide fraction was isolated from the supernatant above (termed L1 fraction) after removal of nucleic acids. DEAE-cellulose chromatography indicated that this material is not a polysaccharide but rather a water-soluble lipopolysaccharide containing strain-specific constituents such as uronic acids, amino acids, amino sugars, neutral sugars, ethanolamine and phosphate, depending on the strain from which lipopolysaccharide II was isolated.", "contents": "The isolation of two different lipopolysaccharide fractions from various Proteus mirabilis strains. Four distinct Proteus mirabilis strains were extracted by the phenol/water procedure. After ultracentrifugation of the dialyzed water phase, the pelleted lipopolysaccharide was purified and analyzed. The sugar composition of this lipopolysaccharide fraction I was similar for all four strains, containing only small amounts of strain-specific constituents. A second lipopolysaccharide fraction was isolated from the supernatant above (termed L1 fraction) after removal of nucleic acids. DEAE-cellulose chromatography indicated that this material is not a polysaccharide but rather a water-soluble lipopolysaccharide containing strain-specific constituents such as uronic acids, amino acids, amino sugars, neutral sugars, ethanolamine and phosphate, depending on the strain from which lipopolysaccharide II was isolated."}
{"id": "PMID:1102310", "title": "Identification of ribitol phosphate as a constituent of the lipopolysaccharide from Proteus mirabilis, Strain D52.", "content": "A polyol was released from the lipopolysaccharide of Proteus mirabilis, strain D52, during alkaline hydrolysis and its phosphate ester was isolated after acid hydrolysis. This polyol has been identified as ribitol by comparison of the free polyol, its phosphate ester and its anhydro derivative formed after acid treatment with authentic xylitol, D- and L-arabitol, ribitol and their corresponding derivatives on paper and gas-liquid chromatography.", "contents": "Identification of ribitol phosphate as a constituent of the lipopolysaccharide from Proteus mirabilis, Strain D52. A polyol was released from the lipopolysaccharide of Proteus mirabilis, strain D52, during alkaline hydrolysis and its phosphate ester was isolated after acid hydrolysis. This polyol has been identified as ribitol by comparison of the free polyol, its phosphate ester and its anhydro derivative formed after acid treatment with authentic xylitol, D- and L-arabitol, ribitol and their corresponding derivatives on paper and gas-liquid chromatography."}
{"id": "PMID:1102312", "title": "Function of the duct-ligated canine pancreatic allograft.", "content": "Heterotopic transplantation of a duct-ligated canine pancreas was performed in 20 previously pancreatectomized, non-related recipient dogs. A technical procedure was elaborated for this grafting. It was found that early, fatal postoperative bleeding in the recipient could be avoided when the interval between pancreatectomy and transplantation was reduced from 7 to 2-4 days. The early endocrine function was studied in 15 surviving animals by determination of blood glucose and serum insulin during fasting and during glucose load. 24 h after transplantation the blood glucose values were normal and 4-8 days after transplantation the glucose tolerance was within normal limits. The serum insulin was on a supranormal level, also during glucose load. The early endocrine capacity of the transplanted duct-ligated pancreas after transplantation was found to be satisfactory, restoring the blood glucose in the pancreatectomized recipients to normal levels.", "contents": "Function of the duct-ligated canine pancreatic allograft. Heterotopic transplantation of a duct-ligated canine pancreas was performed in 20 previously pancreatectomized, non-related recipient dogs. A technical procedure was elaborated for this grafting. It was found that early, fatal postoperative bleeding in the recipient could be avoided when the interval between pancreatectomy and transplantation was reduced from 7 to 2-4 days. The early endocrine function was studied in 15 surviving animals by determination of blood glucose and serum insulin during fasting and during glucose load. 24 h after transplantation the blood glucose values were normal and 4-8 days after transplantation the glucose tolerance was within normal limits. The serum insulin was on a supranormal level, also during glucose load. The early endocrine capacity of the transplanted duct-ligated pancreas after transplantation was found to be satisfactory, restoring the blood glucose in the pancreatectomized recipients to normal levels."}
{"id": "PMID:1102311", "title": "The psychiatric aspects of cardiac intensive therapy: a review.", "content": "The post cardiotomy state is typically delirious and although organic factors are important it is multi-determined. Cerebral ischaemia has been implicated in the development of psychological disorder after resuscitation but longer term neurotic disorders also occur. Affective disturbances, particularly depression, are associated with the coronary care experience. The following conditions are directly related to an increased incidence of psychological disorder: age, loss of sleep, sensory deprivation, stressful experiences, pre-operative morbidity (both physical and mental), the severity of both surgical trauma and the post-operative medical state. For both the staff who administer intensive therapy and the patient who receives it there are unique psychological hazards, the management of which depends largely on mutual understanding and support.", "contents": "The psychiatric aspects of cardiac intensive therapy: a review. The post cardiotomy state is typically delirious and although organic factors are important it is multi-determined. Cerebral ischaemia has been implicated in the development of psychological disorder after resuscitation but longer term neurotic disorders also occur. Affective disturbances, particularly depression, are associated with the coronary care experience. The following conditions are directly related to an increased incidence of psychological disorder: age, loss of sleep, sensory deprivation, stressful experiences, pre-operative morbidity (both physical and mental), the severity of both surgical trauma and the post-operative medical state. For both the staff who administer intensive therapy and the patient who receives it there are unique psychological hazards, the management of which depends largely on mutual understanding and support."}
{"id": "PMID:1102313", "title": "Behaviour of the duct-ligated canine pancreas during hypothermic albumin perfusion.", "content": "The behaviour of eight duct-ligated canine pancreata was studied during 23 hours of continuous, hypothermic perfusion, with an albumin electrolyte solution. The organs developed a considerable oedema during perfusion. This did not seem to influence on the vascular resistance of the organs. A continuous insulin release, a moderate increase in LDH concentration and only small changes in the electrolyte concentrations were observed in the perfusate, indicating only a moderate degree of cellular damage.", "contents": "Behaviour of the duct-ligated canine pancreas during hypothermic albumin perfusion. The behaviour of eight duct-ligated canine pancreata was studied during 23 hours of continuous, hypothermic perfusion, with an albumin electrolyte solution. The organs developed a considerable oedema during perfusion. This did not seem to influence on the vascular resistance of the organs. A continuous insulin release, a moderate increase in LDH concentration and only small changes in the electrolyte concentrations were observed in the perfusate, indicating only a moderate degree of cellular damage."}
{"id": "PMID:1102314", "title": "Twenty-four-hour preservation of the duct-ligated canine pancreatic allograft.", "content": "Twenty previously pancreatectomized dogs received a duct-ligated pancreatic allograft; 16 of the grafts were preserved for 24 h, 9 with continuous hypothermic albumin perfusion, 7 with simple hypothermic storage; 4 glands were transplanted immediately and served as controls. The outcome of preservation was assessed by studying the early endocrine function of the glands. Excluding five early failures due to bleeding, all grafts showed endocrine activity after transplantation and the glucose values were on a normal level. An initial hyperglucemia caused by pancreatectomy disappeared within 24h. In the two groups with 24h preservation, no differences in graft function were found. In the control group, there was a hypoglucemic tendency the first days after transplantation and a prolonged hyperinsulinemia during glucose load. It could be concluded that duct-ligated, isolated canine pancreas well tolerated 24h of preservation both by continuous hypothermic albumin perfusion and by simple hypothermic storage in an extracellular type of fluid.", "contents": "Twenty-four-hour preservation of the duct-ligated canine pancreatic allograft. Twenty previously pancreatectomized dogs received a duct-ligated pancreatic allograft; 16 of the grafts were preserved for 24 h, 9 with continuous hypothermic albumin perfusion, 7 with simple hypothermic storage; 4 glands were transplanted immediately and served as controls. The outcome of preservation was assessed by studying the early endocrine function of the glands. Excluding five early failures due to bleeding, all grafts showed endocrine activity after transplantation and the glucose values were on a normal level. An initial hyperglucemia caused by pancreatectomy disappeared within 24h. In the two groups with 24h preservation, no differences in graft function were found. In the control group, there was a hypoglucemic tendency the first days after transplantation and a prolonged hyperinsulinemia during glucose load. It could be concluded that duct-ligated, isolated canine pancreas well tolerated 24h of preservation both by continuous hypothermic albumin perfusion and by simple hypothermic storage in an extracellular type of fluid."}
{"id": "PMID:1102317", "title": "An improved method for the quantitative analysis of M-mode echocardiograms.", "content": "A computer-assisted system is described which speeds and extends the quantitative interpretation of M-mode echocardiographic recordings. The system consists of a digitizing tablet, minicomputer, TV monitor and a hard copy device. M-mode echocardiograms are placed on the digitizing surface and traced using the digitizing pen. The entered signal includes the endocardial surfaces of the anterior and posterior left ventricular wall for at least one cycle, and two Q waves from a simultaneously recorded ECG to identify end diastole and heart rate. End systole is determined automatically as corresponding to the minimum LV dimension. Results of analysis include continuous plots of estimated volume and circumferential fiber shortening rate (CFSR) vs time. Determinations of special interest are also displayed: enddiastolic volume (EDV) and endsystolic volume (ESV), ejection fraction, cardiac output, mean and peak CFSR. M-mode echocardiograms obtained from 25 normal volunteers are used to evaluate the system. The standard error of the estimate of the computer-assisted system is comparable to the error between observers, furthermore the computer system adds no significant systematic or random error. Comparison between M-mode estimated volumes and angiographically determined values has been described previously and Sy - x here is significantly greater. The main advantages of this system are: 1. a continuous plot of estimated LV volume and CFSR is provided; 2. beat-to-beat analyses are facilitated; 3. the automatic determination of end systole removes possible errors in judgement made previously; 4. it is time saving when one considers the amount of data obtained. With these advantages and the generally satisfactory performance in the clinical trials, this system appears to have extended the clinical quantitative capabilities of M-mode echocardiograms.", "contents": "An improved method for the quantitative analysis of M-mode echocardiograms. A computer-assisted system is described which speeds and extends the quantitative interpretation of M-mode echocardiographic recordings. The system consists of a digitizing tablet, minicomputer, TV monitor and a hard copy device. M-mode echocardiograms are placed on the digitizing surface and traced using the digitizing pen. The entered signal includes the endocardial surfaces of the anterior and posterior left ventricular wall for at least one cycle, and two Q waves from a simultaneously recorded ECG to identify end diastole and heart rate. End systole is determined automatically as corresponding to the minimum LV dimension. Results of analysis include continuous plots of estimated volume and circumferential fiber shortening rate (CFSR) vs time. Determinations of special interest are also displayed: enddiastolic volume (EDV) and endsystolic volume (ESV), ejection fraction, cardiac output, mean and peak CFSR. M-mode echocardiograms obtained from 25 normal volunteers are used to evaluate the system. The standard error of the estimate of the computer-assisted system is comparable to the error between observers, furthermore the computer system adds no significant systematic or random error. Comparison between M-mode estimated volumes and angiographically determined values has been described previously and Sy - x here is significantly greater. The main advantages of this system are: 1. a continuous plot of estimated LV volume and CFSR is provided; 2. beat-to-beat analyses are facilitated; 3. the automatic determination of end systole removes possible errors in judgement made previously; 4. it is time saving when one considers the amount of data obtained. With these advantages and the generally satisfactory performance in the clinical trials, this system appears to have extended the clinical quantitative capabilities of M-mode echocardiograms."}
{"id": "PMID:1102318", "title": "Plasma catecholamines and blood substrate concentrations: studies in insulin induced hypoglycaemia and after adrenaline infusions.", "content": "Plasma adrenaline-blood glucose interrelationships in insulin-induced hypoglycaemia in man have been studied using a sensitive double-isotope derivative method for adrenaline estimation. Plasma adrenaline reached a peak of 1.24 ng/ml at 45 minutes after insulin while blood glucose reached a nadir of 22 mg/100 ml at 30 minutes. There was a strong correlation both between the rise in adrenaline and the degree of hypoglycaemia and between the rise in adrenaline and the post-hypoglycaemic rise in glucose. Plasma noradrenaline rose from 0.29 to 0.59 ng/ml, the rise correlating with the rise in adrenaline. Changes in pulse rate preceded and were unrelated to changes in plasma catecholamines. Fuel mobilisation in response to adrenaline infusion (6 mug/min. for 20 min.) in normoglycaemic man was also studied. Plasma adrenaline concentration rose from a mean of 0.02 ng/ml to 0.71 ng/ml while plasma noradrenaline concentration was unchanged. Blood glucose rose from 71 to 98 mg/100 ml while plasma insulin decreased from 11 to 8 muU/ml. Blood lactate rose by 0.85 mM while pyruvate concentration remained unchanged. Blood glycerol concentration rose twofold and ketone body concentration threefold but there was little change in the concentrations of the glucogenic amino acids, alanine, glutamate and glutamine. Both the 3-hydroxybutyrate/acetoacetate ratio and the lactate/pyruvate ratio rose implying a more reduced intracellular state due presumably to increased hepatic fatty acid oxidation. It is concluded that adrenaline enhances the recycling of lactate and spares glucose through the mobilitsation of lipids but that amino acids are little affected.", "contents": "Plasma catecholamines and blood substrate concentrations: studies in insulin induced hypoglycaemia and after adrenaline infusions. Plasma adrenaline-blood glucose interrelationships in insulin-induced hypoglycaemia in man have been studied using a sensitive double-isotope derivative method for adrenaline estimation. Plasma adrenaline reached a peak of 1.24 ng/ml at 45 minutes after insulin while blood glucose reached a nadir of 22 mg/100 ml at 30 minutes. There was a strong correlation both between the rise in adrenaline and the degree of hypoglycaemia and between the rise in adrenaline and the post-hypoglycaemic rise in glucose. Plasma noradrenaline rose from 0.29 to 0.59 ng/ml, the rise correlating with the rise in adrenaline. Changes in pulse rate preceded and were unrelated to changes in plasma catecholamines. Fuel mobilisation in response to adrenaline infusion (6 mug/min. for 20 min.) in normoglycaemic man was also studied. Plasma adrenaline concentration rose from a mean of 0.02 ng/ml to 0.71 ng/ml while plasma noradrenaline concentration was unchanged. Blood glucose rose from 71 to 98 mg/100 ml while plasma insulin decreased from 11 to 8 muU/ml. Blood lactate rose by 0.85 mM while pyruvate concentration remained unchanged. Blood glycerol concentration rose twofold and ketone body concentration threefold but there was little change in the concentrations of the glucogenic amino acids, alanine, glutamate and glutamine. Both the 3-hydroxybutyrate/acetoacetate ratio and the lactate/pyruvate ratio rose implying a more reduced intracellular state due presumably to increased hepatic fatty acid oxidation. It is concluded that adrenaline enhances the recycling of lactate and spares glucose through the mobilitsation of lipids but that amino acids are little affected."}
{"id": "PMID:1102319", "title": "Insulin metabolism, insulin sensitivity and hormonal responses to insulin infusion in patients taking oral contraceptive steroids.", "content": "The metabolism of unlabelled human monocomponent insulin was studied in a group of six patients being treated with combined oestrogen-progestogen oral contraceptives (OC) and compared with a group of ten normal subjects. The parameters of insulin metabolism were determined by a priming dose-continuous infusion technique which enabled measurements of metabolic clearance rate (MCR) of insulin to be made at four separate steady state hormone concentrations spanning the physiological range. In normal subjects MCR was greatest at low insulin concentrations, falling from 24.7 ml/kg/min. at 16 muU/ml to 11.4 ml/kg/min. at a mean concentration of 280 muU/ml. In the OC group, MCR averaged 20.5 ml/kg/min. and did not change with increasing plasma insulin concentration. The plasma half-disappearance time (T 1/2) was longer than normal in the OC group (5.6 vs. 4.4 min., p less than 0.05) despite a higher MCR. The prolonged T 1/2 indicated that the apparent distribution space was increased in those on OC (166.6 vs. 82.7 mg/kg., p less than 0.0025). The results are interpreted as indicating increased capillary permeability to insulin and increased peripheral degradation. The fact that MCR did not fall in the OC group with increasing plasma insulin concentrations whereas it did in normal subjects, suggested that OC leads to the loss of saturable component of insulin degradation that is present in normal subjects. Insulin sensitivity (as judged by induced hypoglycaemia) was reduced in the OC group while growth hormone responses were within the normal range. Plasma cortisol was increased in those taking OC but the response to insulin induced hyperglycaemia was less marked than normal. The results indicate a significant alteration in insulin metabolism in these subjects, which may contribute to the impairment of carbohydrate tolerance seen in some women taking combined OC.", "contents": "Insulin metabolism, insulin sensitivity and hormonal responses to insulin infusion in patients taking oral contraceptive steroids. The metabolism of unlabelled human monocomponent insulin was studied in a group of six patients being treated with combined oestrogen-progestogen oral contraceptives (OC) and compared with a group of ten normal subjects. The parameters of insulin metabolism were determined by a priming dose-continuous infusion technique which enabled measurements of metabolic clearance rate (MCR) of insulin to be made at four separate steady state hormone concentrations spanning the physiological range. In normal subjects MCR was greatest at low insulin concentrations, falling from 24.7 ml/kg/min. at 16 muU/ml to 11.4 ml/kg/min. at a mean concentration of 280 muU/ml. In the OC group, MCR averaged 20.5 ml/kg/min. and did not change with increasing plasma insulin concentration. The plasma half-disappearance time (T 1/2) was longer than normal in the OC group (5.6 vs. 4.4 min., p less than 0.05) despite a higher MCR. The prolonged T 1/2 indicated that the apparent distribution space was increased in those on OC (166.6 vs. 82.7 mg/kg., p less than 0.0025). The results are interpreted as indicating increased capillary permeability to insulin and increased peripheral degradation. The fact that MCR did not fall in the OC group with increasing plasma insulin concentrations whereas it did in normal subjects, suggested that OC leads to the loss of saturable component of insulin degradation that is present in normal subjects. Insulin sensitivity (as judged by induced hypoglycaemia) was reduced in the OC group while growth hormone responses were within the normal range. Plasma cortisol was increased in those taking OC but the response to insulin induced hyperglycaemia was less marked than normal. The results indicate a significant alteration in insulin metabolism in these subjects, which may contribute to the impairment of carbohydrate tolerance seen in some women taking combined OC."}
{"id": "PMID:1102322", "title": "A stereotaxic headholder for visual neurophysiology.", "content": "A stereotaxic headholder is presented that offers the following advantages: 1. It is especially suitable for visual neurophysiology, because the eye and surrounding orbit are not affected by bars or clamps. 2. It is easy to handle, because the ear bars are mounted on movable slides above the main frame. 3. It is versatile and can be used without additional adapters for different species of animals.", "contents": "A stereotaxic headholder for visual neurophysiology. A stereotaxic headholder is presented that offers the following advantages: 1. It is especially suitable for visual neurophysiology, because the eye and surrounding orbit are not affected by bars or clamps. 2. It is easy to handle, because the ear bars are mounted on movable slides above the main frame. 3. It is versatile and can be used without additional adapters for different species of animals."}
{"id": "PMID:1102320", "title": "Synaptosomal uptake and levels of serotonin in rat brain areas after p-chloroamphetamine or B-9 lesions.", "content": "In contrast to the pronounced fall in 5HT levels and synaptosomal uptake caused by p-chloroamphetamine, bilateral lesions of the B-9 cell group caused minimal regional changes, except for 35% decreases in the metathalamus-thalamus. We conclude therefore that the prolonged biochemical effects of p-chloroamphetamine are not due to a selective cytotoxic action on B-9 cells; and that a lateral 5HT pathway, possibly from the B-9 cell group, projects to the metathalamus-thalamus.", "contents": "Synaptosomal uptake and levels of serotonin in rat brain areas after p-chloroamphetamine or B-9 lesions. In contrast to the pronounced fall in 5HT levels and synaptosomal uptake caused by p-chloroamphetamine, bilateral lesions of the B-9 cell group caused minimal regional changes, except for 35% decreases in the metathalamus-thalamus. We conclude therefore that the prolonged biochemical effects of p-chloroamphetamine are not due to a selective cytotoxic action on B-9 cells; and that a lateral 5HT pathway, possibly from the B-9 cell group, projects to the metathalamus-thalamus."}
{"id": "PMID:1102328", "title": "[Correlation of the bioelectrical activity and energy metabolism in various parts of the dog brain in the process of ontogeny under ether anesthetic conditions].", "content": "Experiments conducted on 50 puppies of 2-days, 2-weeks and a month old evidenced that the maturing of the central nervous system in puppies was accompanied by a progressive growth of the bioelectric activity and of the energy exchange forse. With advancing age of the animals the inhibitory action of ether on the oxygen uptake by the tissues of the visual, auditory cortical zones and of the medial thalamus along with the biocurrents in these structures were on a decline. At the same time, as the post-natal development of the animals went on, the processes of oxidative phosphorylation in the cortical segments were disturbed greater than at the 2-week age. In 2-day old animals it did not change substantially by comparison with controls.", "contents": "[Correlation of the bioelectrical activity and energy metabolism in various parts of the dog brain in the process of ontogeny under ether anesthetic conditions]. Experiments conducted on 50 puppies of 2-days, 2-weeks and a month old evidenced that the maturing of the central nervous system in puppies was accompanied by a progressive growth of the bioelectric activity and of the energy exchange forse. With advancing age of the animals the inhibitory action of ether on the oxygen uptake by the tissues of the visual, auditory cortical zones and of the medial thalamus along with the biocurrents in these structures were on a decline. At the same time, as the post-natal development of the animals went on, the processes of oxidative phosphorylation in the cortical segments were disturbed greater than at the 2-week age. In 2-day old animals it did not change substantially by comparison with controls."}
{"id": "PMID:1102329", "title": "[Experimental and clinical study of d, 1-oxyphedrin].", "content": "Comparative pharmacological and toxicological studies were carried out on animals covering the preparation d,1-oxyphedrine (myophedrine), put out in the GDR, as against the drug 1-oxyphedrine. The activity of both drugs showed no material difference. Clinical trials demonstrated good tolerance of the drug both by healthy persons and patients with its intravenous administration twice a day in amounts of 1 mg, or by mouth--3 times a day in amounts of 8--16 mg. In outpatients with anginal pains after sustained myocardial infarction and also in institutionally treated patients with myocardial infarction the activity of d,1-oxyphedrine proved to be not inferior to that of 1-oxyphedrine.", "contents": "[Experimental and clinical study of d, 1-oxyphedrin]. Comparative pharmacological and toxicological studies were carried out on animals covering the preparation d,1-oxyphedrine (myophedrine), put out in the GDR, as against the drug 1-oxyphedrine. The activity of both drugs showed no material difference. Clinical trials demonstrated good tolerance of the drug both by healthy persons and patients with its intravenous administration twice a day in amounts of 1 mg, or by mouth--3 times a day in amounts of 8--16 mg. In outpatients with anginal pains after sustained myocardial infarction and also in institutionally treated patients with myocardial infarction the activity of d,1-oxyphedrine proved to be not inferior to that of 1-oxyphedrine."}
{"id": "PMID:1102338", "title": "Nutritional application and implication of 1,3-butanediol.", "content": "Research in the United States on synthetic sources of dietary calories was initiated in 1958 to develop high nutrient density food for extended manned space travel. Of many known compounds screened, 1,3-butanediol was the most promising. Small amounts in ester form with fatty acids exist in nature, and tests indicate a low acute oral and chronic toxicity similar to that of propylene glycol or glycerol. Multi-generation reproduction, teratological, and mutagen studies have revealed nothing detrimental. Following an adaptation period, 1,3-butanediol furnishes approximately 6 kcal/g if fed at levels not exceeding 20% in the diet of rats. Higher levels result in an impairment in growth and food utilization. In young animals, body fat stores appear to be lessened, as is resistance to the stress of extreme cold. However, dogs fed 20% 1,3-butanediol can maintain sustained muscular work on treadmills, but larger amounts can result in incoordination due to a narcotic effect common to glycols. Little research has been conducted on the behavioral effects of large doses. At present, 1,3-butanediol is used mainly as a solvent for food flavors. If the unpleasant taste problem can be overcome and if given FDA approval, 1,3-butanediol may have an increased role in our food supply as a functional food additive, preservative, and source of calories for man and animals.", "contents": "Nutritional application and implication of 1,3-butanediol. Research in the United States on synthetic sources of dietary calories was initiated in 1958 to develop high nutrient density food for extended manned space travel. Of many known compounds screened, 1,3-butanediol was the most promising. Small amounts in ester form with fatty acids exist in nature, and tests indicate a low acute oral and chronic toxicity similar to that of propylene glycol or glycerol. Multi-generation reproduction, teratological, and mutagen studies have revealed nothing detrimental. Following an adaptation period, 1,3-butanediol furnishes approximately 6 kcal/g if fed at levels not exceeding 20% in the diet of rats. Higher levels result in an impairment in growth and food utilization. In young animals, body fat stores appear to be lessened, as is resistance to the stress of extreme cold. However, dogs fed 20% 1,3-butanediol can maintain sustained muscular work on treadmills, but larger amounts can result in incoordination due to a narcotic effect common to glycols. Little research has been conducted on the behavioral effects of large doses. At present, 1,3-butanediol is used mainly as a solvent for food flavors. If the unpleasant taste problem can be overcome and if given FDA approval, 1,3-butanediol may have an increased role in our food supply as a functional food additive, preservative, and source of calories for man and animals."}
{"id": "PMID:1102339", "title": "Studies on sperm antigenicity. 2. In vivo and in vitro cellular reactivity in guinea pigs sensitized to homologous and heterologous spermatozoal autoantigens.", "content": "In vitro delayed type hypersensitivity was demonstrated with peritoneal exudate cells from guinea pigs of the Rockefeller and Hartley strains, immunized with different preparations of the guinea pig male reproductive tract (RMT) emulsified in complete Freund's adjuvant-H37Ra. The RMT preparations were purified guinea pig spermatozoal autoantigens S, P, and T; whole guinea pig spermatozoa, and extract from epididymal tissue. The cellular sensitivity in vivo was demonstrated by injecting the proper antigen into the skin of the tested animals and in vitro by the macrophage inhibition technique. Peritoneal exudate cells from guinea pigs sensitized with whole guinea pig spermatozoa cells were inhibited in vitro by the specific antigen, epididymal extract, and autoantigen-T. Autoantigen-S was found to be a weak immunogen. However, the migration of peritoneal exudate cells from guinea pigs sensitized with large amounts of antigen-S was inhibited by whole spermatozoa in vitro. This cross-reactivity revealed the possibility that the immunogenicity of purified autoantigen-S might be connected to its molecular size. According to the immunizing dose of the antigens, testicular lesions of either the aspermatogenic or orchitis type were found in the testes of sensitized guinea pigs. Lesions in the testes of the guinea pigs were not detectable by cross-immunization with heterologous human or rat spermatozoa, although some degree of in vitro cross-reactivity was detected by skin test studies.", "contents": "Studies on sperm antigenicity. 2. In vivo and in vitro cellular reactivity in guinea pigs sensitized to homologous and heterologous spermatozoal autoantigens. In vitro delayed type hypersensitivity was demonstrated with peritoneal exudate cells from guinea pigs of the Rockefeller and Hartley strains, immunized with different preparations of the guinea pig male reproductive tract (RMT) emulsified in complete Freund's adjuvant-H37Ra. The RMT preparations were purified guinea pig spermatozoal autoantigens S, P, and T; whole guinea pig spermatozoa, and extract from epididymal tissue. The cellular sensitivity in vivo was demonstrated by injecting the proper antigen into the skin of the tested animals and in vitro by the macrophage inhibition technique. Peritoneal exudate cells from guinea pigs sensitized with whole guinea pig spermatozoa cells were inhibited in vitro by the specific antigen, epididymal extract, and autoantigen-T. Autoantigen-S was found to be a weak immunogen. However, the migration of peritoneal exudate cells from guinea pigs sensitized with large amounts of antigen-S was inhibited by whole spermatozoa in vitro. This cross-reactivity revealed the possibility that the immunogenicity of purified autoantigen-S might be connected to its molecular size. According to the immunizing dose of the antigens, testicular lesions of either the aspermatogenic or orchitis type were found in the testes of sensitized guinea pigs. Lesions in the testes of the guinea pigs were not detectable by cross-immunization with heterologous human or rat spermatozoa, although some degree of in vitro cross-reactivity was detected by skin test studies."}
{"id": "PMID:1102340", "title": "Immunologic studies of male infertility.", "content": "Infertile men with azo- or oligospermia of unknown cause were investigated for evidence of testicular autoimmunity. Testicular germinal cell antibodies were found in 14% of the patients, compared with 5% of normal men, and 21% had spermatozoal antibodies, compared with 5% of the normal subjects. One-third had positive macrophage inhibitory factor tests, compared with 5% of normal subjects. However, of autoantibodies against thyroid, stomach, and nuclear material, only the prevalence of thyroid cytoplasmic antibodies was significantly greater than in normal subjects; serum IgG, IgM, and IgA levels were normal in all cases tested. Furthermore, there was no excess of lymphoid tissue on biopsy and no evidence of antibody deposition in the testicular tissue. The evidence for autoimmunity is less impressive than that for leprous orchitis, which has been proposed as a model for testicular organ-specific autoimmunity. Nevertheless, it is possible that certain germinal cell or spermatozoal antibodies may be directed against factors necessary for orderly spermatogenesis. If so, they may play a role in some cases of maturation arrest and shedding defects.", "contents": "Immunologic studies of male infertility. Infertile men with azo- or oligospermia of unknown cause were investigated for evidence of testicular autoimmunity. Testicular germinal cell antibodies were found in 14% of the patients, compared with 5% of normal men, and 21% had spermatozoal antibodies, compared with 5% of the normal subjects. One-third had positive macrophage inhibitory factor tests, compared with 5% of normal subjects. However, of autoantibodies against thyroid, stomach, and nuclear material, only the prevalence of thyroid cytoplasmic antibodies was significantly greater than in normal subjects; serum IgG, IgM, and IgA levels were normal in all cases tested. Furthermore, there was no excess of lymphoid tissue on biopsy and no evidence of antibody deposition in the testicular tissue. The evidence for autoimmunity is less impressive than that for leprous orchitis, which has been proposed as a model for testicular organ-specific autoimmunity. Nevertheless, it is possible that certain germinal cell or spermatozoal antibodies may be directed against factors necessary for orderly spermatogenesis. If so, they may play a role in some cases of maturation arrest and shedding defects."}
{"id": "PMID:1102341", "title": "Inhibitory activity of four analogs of luteinizing hormone-releasing hormone in vivo.", "content": "Four analogs of luteinizing hormone-releasing hormone (LH-RH), [des-His2,D-Ala6]-LH-RH, [des-His2,D-Ala6, des-Gly-NH2(10)1-LH-RH ethylamide, [des-His2,D-Leu6]-LH-RH, and [D-Phe2,D-Leu6]-LH-RH, at 300-fold molar ratios (analog/LH-RH) led to an almost complete inhibition of LH response to LH-RH in anesthetized 4-day cycling rats on the afternoon of proestrus. At a 75-fold molar ratio, [des-His2,D-Ala6]-LH-RH still inhibited the LH-RH-induced LH release by 50%. The ethylamide substitution at the COOH terminus of [des-His2,D-Ala6]-LH-RH did not significantly improve the inhibitory activity of the molecule.", "contents": "Inhibitory activity of four analogs of luteinizing hormone-releasing hormone in vivo. Four analogs of luteinizing hormone-releasing hormone (LH-RH), [des-His2,D-Ala6]-LH-RH, [des-His2,D-Ala6, des-Gly-NH2(10)1-LH-RH ethylamide, [des-His2,D-Leu6]-LH-RH, and [D-Phe2,D-Leu6]-LH-RH, at 300-fold molar ratios (analog/LH-RH) led to an almost complete inhibition of LH response to LH-RH in anesthetized 4-day cycling rats on the afternoon of proestrus. At a 75-fold molar ratio, [des-His2,D-Ala6]-LH-RH still inhibited the LH-RH-induced LH release by 50%. The ethylamide substitution at the COOH terminus of [des-His2,D-Ala6]-LH-RH did not significantly improve the inhibitory activity of the molecule."}
{"id": "PMID:1102342", "title": "Gonadotropin-releasing activity of two highly active and long-acting analogs of luteinizing hormone-releasing hormone after subcutaneous, intravaginal, and oral administration.", "content": "The gonadotropin-releasing activities of two synthetic analogs of luteinizing hormone-releasing hormone (LH-RH), D-Ala6-des-Gly10-LH-RH ethylamide and D-Leu6-des-Gly10-LH-RH ethylamide were evaluated in immature female rats after subcutaneous, intravaginal, and oral administration. Maximal peaks of serum LH and follicle-stimulating hormone (FSH) levels after administration of both analogs by any of the three routes were obtained at 2 hours. Therefore, serum gonadotropin levels declined slowly, so that at 6 hours LH levels had returned to base line values, whereas FSH levels remained elevated for up to 10 hours. The integrated serum gaondotropin levels after LH-RH and both analogs over a 10-hour period indicated that D-Leu6-des-Gly10-LH-RH EA and D-Ala6-des-Gly10-LH-RH EA released more LH and FSH than did the LH-RH decapeptide. The intense and long-acting properties of these analogs in releasing LH and FSH suggest the possibility that they may be more useful therapeutically than LH-RH.", "contents": "Gonadotropin-releasing activity of two highly active and long-acting analogs of luteinizing hormone-releasing hormone after subcutaneous, intravaginal, and oral administration. The gonadotropin-releasing activities of two synthetic analogs of luteinizing hormone-releasing hormone (LH-RH), D-Ala6-des-Gly10-LH-RH ethylamide and D-Leu6-des-Gly10-LH-RH ethylamide were evaluated in immature female rats after subcutaneous, intravaginal, and oral administration. Maximal peaks of serum LH and follicle-stimulating hormone (FSH) levels after administration of both analogs by any of the three routes were obtained at 2 hours. Therefore, serum gonadotropin levels declined slowly, so that at 6 hours LH levels had returned to base line values, whereas FSH levels remained elevated for up to 10 hours. The integrated serum gaondotropin levels after LH-RH and both analogs over a 10-hour period indicated that D-Leu6-des-Gly10-LH-RH EA and D-Ala6-des-Gly10-LH-RH EA released more LH and FSH than did the LH-RH decapeptide. The intense and long-acting properties of these analogs in releasing LH and FSH suggest the possibility that they may be more useful therapeutically than LH-RH."}
{"id": "PMID:1102343", "title": "Immune capacity of mice tolerating a weakly incompatible skin allograft. I. Tolerance induction by neonatal skin graft in adult recipients or by neonatal inoculation of adult cells.", "content": "An analysis was made of the so-called \"tolergenic\" effect of neonatal skin grafts which tend to survive longer than control adult grafts and confer this tendency also on simultaneously transplanted adult grafts of the same genotype. When the time interval between the neonatal and adult grafts varied from zero to 150 days, it could be demonstrated that neonatal skin grafts are capable of inducing second-set response comparable to that induced by adult grafts provided that the adult graft is not given simultaneously but only following 20 days or later. Signs of immune erises (i.e. various degrees of a round-cell infiltration) were, however, detected histologically even with shorter intervals of time. The involvement of some non-specific components in the early postgrafting period was indicated by a similar effect of sera from recipients of syngeneic grafts and MSA-incompatible grafts on the survival of grafts across the male-specific antigen barrier. Only long surviving allografts tend to specifically reduce the recipient's immune responsiveness. The possible mechanisms of the specific and non-specific components in the effect of neonatal skin grafts and the factor of time are discussed.", "contents": "Immune capacity of mice tolerating a weakly incompatible skin allograft. I. Tolerance induction by neonatal skin graft in adult recipients or by neonatal inoculation of adult cells. An analysis was made of the so-called \"tolergenic\" effect of neonatal skin grafts which tend to survive longer than control adult grafts and confer this tendency also on simultaneously transplanted adult grafts of the same genotype. When the time interval between the neonatal and adult grafts varied from zero to 150 days, it could be demonstrated that neonatal skin grafts are capable of inducing second-set response comparable to that induced by adult grafts provided that the adult graft is not given simultaneously but only following 20 days or later. Signs of immune erises (i.e. various degrees of a round-cell infiltration) were, however, detected histologically even with shorter intervals of time. The involvement of some non-specific components in the early postgrafting period was indicated by a similar effect of sera from recipients of syngeneic grafts and MSA-incompatible grafts on the survival of grafts across the male-specific antigen barrier. Only long surviving allografts tend to specifically reduce the recipient's immune responsiveness. The possible mechanisms of the specific and non-specific components in the effect of neonatal skin grafts and the factor of time are discussed."}
{"id": "PMID:1102330", "title": "[Effect of sodium salicylate on the cerebral blood circulation (an experimental and clinical study)].", "content": "The effect of sodium salicylate (100 mg/kg intravenously) on the circulation rate of the cerebral blood flow, total arterial pressure, oxygen tension in the cerebral tissue (by the polarographic method) and on the pressure in the brain venous vessels was studied on anesthetized cats with controlled respiration. In rheumatic patients the action of the drug (10 ml of a 10% solution, intravenously) on the rheoencephalographic findings and the total arterial pressure was investigated. Sodium salicylate was shown to raise the tonicity of the intra- and extracranial vessels, to lower the amplitude of the pulse fluctuations and to highten the total arterial pressure. The cerebral circulation depends upon changes in the tonicity of the cerebral vessels and on the total arterial pressure, i. e. with a significant rise of the latter it increases in spite of a greater vascular resistance. Changes in the blood flow are followed by corresponding variations in the PO2 of the brain tissue. No significant changes of the pressure in the venous system of the brain could be established.", "contents": "[Effect of sodium salicylate on the cerebral blood circulation (an experimental and clinical study)]. The effect of sodium salicylate (100 mg/kg intravenously) on the circulation rate of the cerebral blood flow, total arterial pressure, oxygen tension in the cerebral tissue (by the polarographic method) and on the pressure in the brain venous vessels was studied on anesthetized cats with controlled respiration. In rheumatic patients the action of the drug (10 ml of a 10% solution, intravenously) on the rheoencephalographic findings and the total arterial pressure was investigated. Sodium salicylate was shown to raise the tonicity of the intra- and extracranial vessels, to lower the amplitude of the pulse fluctuations and to highten the total arterial pressure. The cerebral circulation depends upon changes in the tonicity of the cerebral vessels and on the total arterial pressure, i. e. with a significant rise of the latter it increases in spite of a greater vascular resistance. Changes in the blood flow are followed by corresponding variations in the PO2 of the brain tissue. No significant changes of the pressure in the venous system of the brain could be established."}
{"id": "PMID:1102344", "title": "Cyclophosphamide-induced immunological tolerance: recovery leads to a memory state.", "content": "Tolerance to SRBC has been induced in adult mice by the aid of cyclophosphamide, and the duration of the tolerant state has been tested by a second injection of antigen at different times thereafter. As evidenced by 19S and 7S PFC 4 days after challenge, tolerance in this model is of relatively short duration and leads spontaneously to a memory state.", "contents": "Cyclophosphamide-induced immunological tolerance: recovery leads to a memory state. Tolerance to SRBC has been induced in adult mice by the aid of cyclophosphamide, and the duration of the tolerant state has been tested by a second injection of antigen at different times thereafter. As evidenced by 19S and 7S PFC 4 days after challenge, tolerance in this model is of relatively short duration and leads spontaneously to a memory state."}
{"id": "PMID:1102345", "title": "[LH and FSH responses to synthetic LH-RH in various estradiol levels of anovulatory women (author's transl)].", "content": "Synthetic LH releasing hormone (LH-RH) is effective in increasing both serum LH and FSH in humans. Therefore, LH-RH is used to clarify the function of the hypothalamic pituitary ovarian axis in adult women. Variations in response to synthetic LH-RH during different phases of the menstrual cycle are known. These variations may be due to changes in the ovarian secretion of sex streoid hormones during the menstrual cycle. In the present study, effects of serum estradiol (Ed) on pituitary gonadotropin response to synthetic LH-RH were studied in 55 subjects without ovulation. Serum levels of LH, FSH and Ed were determined before and at 30 min after intramuscular administration of 100 mug LH-RH. LH and FSH levels in the serum were measured by the double antibody method of radioimmunoassay. Ed levels were determined by the radioimmunoassay method of T. Makino, using the antiserum against Ed-6-oxime-BSA donated by Dr. T. Makino and applied to the microcolumn of sephadex LH 20. Preliminary evidence about Ed levels during the normal menstrual cycle and in anovulatory women suggested that an Ed level under 30 pg/ml indicates the presence of a small amount of Ed. Therefore, 55 patients were divided into 2 groups; the low Ed group that showed low basal Ed levels (under 30 pg/ml) and the Ed normal group that showed normal basal Ed levels (over 30 pg/ml). Pituitary gonadotropin responses to synthetic LH-RH were studied in these two groups. No statistical difference was shown between the two groups studied for basal LH level and FSH level. After LH-RH injection in the Ed normal group, serum LH level was significantly higher and serum FSH level was significantly lower than that in the low Ed group. Then, in order to clarify the difference in two groups, the ratio of LH to FSH (LH/FSH) before and after administration of LH-RH was examined. The ratio in the Ed normal group was significantly higher than one in the low Ed group before and after administration of LH-RH. Moreover, the percentage increase of the ratio was significantly larger in the Ed normal group than that in the low Ed group. A positive correlation (r=0.4228, p less than 0.01) was found between LH/FSH following administration of LH-RH and circulating the Ed level. These results suggest that the response of pituitary gonadotropins to LH-RH is changed by serum Ed and that serum Ed causes a differential release of LH and FSH in response to LH-RH. The data seem to demonstrate that LH-RH and serum Ed show a complex interplay in releasing LH and FSH.", "contents": "[LH and FSH responses to synthetic LH-RH in various estradiol levels of anovulatory women (author's transl)]. Synthetic LH releasing hormone (LH-RH) is effective in increasing both serum LH and FSH in humans. Therefore, LH-RH is used to clarify the function of the hypothalamic pituitary ovarian axis in adult women. Variations in response to synthetic LH-RH during different phases of the menstrual cycle are known. These variations may be due to changes in the ovarian secretion of sex streoid hormones during the menstrual cycle. In the present study, effects of serum estradiol (Ed) on pituitary gonadotropin response to synthetic LH-RH were studied in 55 subjects without ovulation. Serum levels of LH, FSH and Ed were determined before and at 30 min after intramuscular administration of 100 mug LH-RH. LH and FSH levels in the serum were measured by the double antibody method of radioimmunoassay. Ed levels were determined by the radioimmunoassay method of T. Makino, using the antiserum against Ed-6-oxime-BSA donated by Dr. T. Makino and applied to the microcolumn of sephadex LH 20. Preliminary evidence about Ed levels during the normal menstrual cycle and in anovulatory women suggested that an Ed level under 30 pg/ml indicates the presence of a small amount of Ed. Therefore, 55 patients were divided into 2 groups; the low Ed group that showed low basal Ed levels (under 30 pg/ml) and the Ed normal group that showed normal basal Ed levels (over 30 pg/ml). Pituitary gonadotropin responses to synthetic LH-RH were studied in these two groups. No statistical difference was shown between the two groups studied for basal LH level and FSH level. After LH-RH injection in the Ed normal group, serum LH level was significantly higher and serum FSH level was significantly lower than that in the low Ed group. Then, in order to clarify the difference in two groups, the ratio of LH to FSH (LH/FSH) before and after administration of LH-RH was examined. The ratio in the Ed normal group was significantly higher than one in the low Ed group before and after administration of LH-RH. Moreover, the percentage increase of the ratio was significantly larger in the Ed normal group than that in the low Ed group. A positive correlation (r=0.4228, p less than 0.01) was found between LH/FSH following administration of LH-RH and circulating the Ed level. These results suggest that the response of pituitary gonadotropins to LH-RH is changed by serum Ed and that serum Ed causes a differential release of LH and FSH in response to LH-RH. The data seem to demonstrate that LH-RH and serum Ed show a complex interplay in releasing LH and FSH."}
{"id": "PMID:1102346", "title": "[A study on the regulatory mechanism of hypothalamo-hypophyseo-ovarian system in estrous cycles of rats: Changes in responsiveness of the anterior pituitary gland to synthetic LH-RH (author's transl)].", "content": "It is generally accepted that the sexual cycle in mammals is accomplished by the systematic coupling of components of the hypothalamo-hypophyseo-gonadal system. However, how these components are related to each other to develop the sexual cyclicity has not yet been clearly established. A gonadotropin releasing factor (GnRF), which has been extracted and purified from porcine hypothalamus and whose molecular structure was determined in 1971, has been synthesized in many laboratories. The synthetic GnRF, named LRF or LH-RH, has gonadotropin releasing activity for many species of mammals, including humans. The debut of the synthetic LRF has given us a pure material and a direct means of stimulating, relatively easily, the gonadotroph cells in the anterior pituitary glands. In the present study, variations in the gonadotropin releasing activity of the pituitary gland in relation to synthetic LRF was investigated throughout the sexual cycle of rats. The relationship between pituitary sensitivity and levels of steroid hormones in the blood is discussed.", "contents": "[A study on the regulatory mechanism of hypothalamo-hypophyseo-ovarian system in estrous cycles of rats: Changes in responsiveness of the anterior pituitary gland to synthetic LH-RH (author's transl)]. It is generally accepted that the sexual cycle in mammals is accomplished by the systematic coupling of components of the hypothalamo-hypophyseo-gonadal system. However, how these components are related to each other to develop the sexual cyclicity has not yet been clearly established. A gonadotropin releasing factor (GnRF), which has been extracted and purified from porcine hypothalamus and whose molecular structure was determined in 1971, has been synthesized in many laboratories. The synthetic GnRF, named LRF or LH-RH, has gonadotropin releasing activity for many species of mammals, including humans. The debut of the synthetic LRF has given us a pure material and a direct means of stimulating, relatively easily, the gonadotroph cells in the anterior pituitary glands. In the present study, variations in the gonadotropin releasing activity of the pituitary gland in relation to synthetic LRF was investigated throughout the sexual cycle of rats. The relationship between pituitary sensitivity and levels of steroid hormones in the blood is discussed."}
{"id": "PMID:1102369", "title": "Antidiabetic action of somatostatin--assessed by the artificial pancreas.", "content": "By means of a glucose-controlled insulin- and glucose-infusion system (GCIGIS) we examined the effect of somatostatin on insulin and glucose requirements following meals or oral glucose loads in juvenile diabetics. In six of seven patients the insulin requirement with somatostatin was remarkably reduced to between 38 per cent and 79 per cent of that of otherwise identical control experiments. No reduction could be found in the seventh case, fed only 575 kcal. In all cases we observed an increase in dextrose demanded from the GCIGIS ranging between 28 per cent and 192 per cent of the control amounts. In addition, a lowering and smoothing of postprandial blood glucose curves caused by somatostatin application was a general finding. It seems to us most likely that the well-known suppression of the secretion of growth hormone and glucagon, both insulin antagonists, is responsible for the antidiabetic action of somatostatin.", "contents": "Antidiabetic action of somatostatin--assessed by the artificial pancreas. By means of a glucose-controlled insulin- and glucose-infusion system (GCIGIS) we examined the effect of somatostatin on insulin and glucose requirements following meals or oral glucose loads in juvenile diabetics. In six of seven patients the insulin requirement with somatostatin was remarkably reduced to between 38 per cent and 79 per cent of that of otherwise identical control experiments. No reduction could be found in the seventh case, fed only 575 kcal. In all cases we observed an increase in dextrose demanded from the GCIGIS ranging between 28 per cent and 192 per cent of the control amounts. In addition, a lowering and smoothing of postprandial blood glucose curves caused by somatostatin application was a general finding. It seems to us most likely that the well-known suppression of the secretion of growth hormone and glucagon, both insulin antagonists, is responsible for the antidiabetic action of somatostatin."}
{"id": "PMID:1102370", "title": "Observations on patients with neural-tube defects in a metropolitan hospital clinic: an epidemiological history.", "content": "A survey of the population of the Spina Bifida Service (107 patients) of Georgetown University Hospital, Washington, D.C., and of a control population in the same hospital revealed that, although situated in an ethnically heterogeneous area, Service patients with a history of neural-tube defects in siblings or family significantly more often had ancestors on both sides from the British Isles than did either isolated cases or the controls. In addition, of 107 families reviewed, nine had more than one affected child, nine had a positive family history for neural-tube defects, and nine of the patients and seven of an estimated total of 220 siblings had an unrelated major birth-defect. These incidences are among the highest published so far. Contrary to other surveys, there was no deficiency of twinning among the sibships, nor of males among the Spina Bifida Service patients. These observations indicate the desirability of studying each population of patients with neural-tube defects in order to provide more realistic counseling and so that clues may be gathered as to the genetic mechanisms and environmental factors which led to the production of such defects.", "contents": "Observations on patients with neural-tube defects in a metropolitan hospital clinic: an epidemiological history. A survey of the population of the Spina Bifida Service (107 patients) of Georgetown University Hospital, Washington, D.C., and of a control population in the same hospital revealed that, although situated in an ethnically heterogeneous area, Service patients with a history of neural-tube defects in siblings or family significantly more often had ancestors on both sides from the British Isles than did either isolated cases or the controls. In addition, of 107 families reviewed, nine had more than one affected child, nine had a positive family history for neural-tube defects, and nine of the patients and seven of an estimated total of 220 siblings had an unrelated major birth-defect. These incidences are among the highest published so far. Contrary to other surveys, there was no deficiency of twinning among the sibships, nor of males among the Spina Bifida Service patients. These observations indicate the desirability of studying each population of patients with neural-tube defects in order to provide more realistic counseling and so that clues may be gathered as to the genetic mechanisms and environmental factors which led to the production of such defects."}
{"id": "PMID:1102371", "title": "Toxic encephalopathy and acute brain-swelling in children.", "content": "Problems in the diagnosis and management of children who present with an acute encephalopathic illness are examined. The aetiology, pathogenesis, pathology and clinical features of acute toxic encephalopathies are discussed, together with the differential diagnosis of those encephalopathies which are not induced by toxicity. Guidance is given on measures for the reduction of raised intracranial pressure and the management of defects of homeostasis. It is essential that facilities for intensive monitoring of these children be available during the acute phase of illness in order to reduce the number surviving with sequelae of mental and physical handicap.", "contents": "Toxic encephalopathy and acute brain-swelling in children. Problems in the diagnosis and management of children who present with an acute encephalopathic illness are examined. The aetiology, pathogenesis, pathology and clinical features of acute toxic encephalopathies are discussed, together with the differential diagnosis of those encephalopathies which are not induced by toxicity. Guidance is given on measures for the reduction of raised intracranial pressure and the management of defects of homeostasis. It is essential that facilities for intensive monitoring of these children be available during the acute phase of illness in order to reduce the number surviving with sequelae of mental and physical handicap."}
{"id": "PMID:1102372", "title": "Foetal-associated material: its expression in long-term cultured human skin.", "content": "Using immunofluorescence and electron microscopy, we have demonstrated the presence of \"foetal substances\" (antigens) in skin kept in tissue culture medium for periods of time ranging from 9 days-18 weeks. This is a specific reaction demonstrated with an anti-human foetal serum raised in New Zealand white rabbits. It is suggested that the expression of foetal substances by cells in the skin under these abnormal conditions might lead to the masking or modification of the normal histo-compatible antigens, enabling such skins to survive transplantation without rejection.", "contents": "Foetal-associated material: its expression in long-term cultured human skin. Using immunofluorescence and electron microscopy, we have demonstrated the presence of \"foetal substances\" (antigens) in skin kept in tissue culture medium for periods of time ranging from 9 days-18 weeks. This is a specific reaction demonstrated with an anti-human foetal serum raised in New Zealand white rabbits. It is suggested that the expression of foetal substances by cells in the skin under these abnormal conditions might lead to the masking or modification of the normal histo-compatible antigens, enabling such skins to survive transplantation without rejection."}
{"id": "PMID:1102373", "title": "Organ culture of foetal rat pancreas. Effects of parabiotic culture with foetal adrenal glands.", "content": "The differentiation and growth of the foetal rat pancreas (20 days postcoitum) was studied in parabiotic organ culture with foetal adrenal tissue. In such co-cultures, characteristic pancreatic morphology was preserved and further acinar cell differentiation was fostered. Acinar cells contiunued to represent about 65% of the total explant volume following short-term incubation. The selective islet cell proliferation, previously observed in control pancreatic explants cultured alone, did not occur when adrenals were co-cultured. In addition, the amylase content of the incubation media and of the explanted pancreatic tissue remained high with adrenal co-culture, while the insulin content of the media and of the explanted tissue was markedly suppressed when compared to control pancreatic explants cultured alone. The effects of the adrenal in maintaining the differentiated acinar component of the pancreas and suppressing media insulin concentration diminished over extended incubation. The addition of adrenals to culture of foetal pancreatic explants after 6 days of control culture (at a time when differentiated acinar cells were not identifiable in the explant) did not result in redifferentiation of the acinar component, but did markedly depress media insulin content. Removal of adrenals after 4 days of co-culture resulted in an immediate rise in media insulin concentration and a rapid decline in pancreatic acinar mass. An adrenal-exocrine pancreatic axis is proposed and it is suggested that foetal adrenal secretions may play an important role in the development of the exocrine pancreas in vivo as well as in vitro.", "contents": "Organ culture of foetal rat pancreas. Effects of parabiotic culture with foetal adrenal glands. The differentiation and growth of the foetal rat pancreas (20 days postcoitum) was studied in parabiotic organ culture with foetal adrenal tissue. In such co-cultures, characteristic pancreatic morphology was preserved and further acinar cell differentiation was fostered. Acinar cells contiunued to represent about 65% of the total explant volume following short-term incubation. The selective islet cell proliferation, previously observed in control pancreatic explants cultured alone, did not occur when adrenals were co-cultured. In addition, the amylase content of the incubation media and of the explanted pancreatic tissue remained high with adrenal co-culture, while the insulin content of the media and of the explanted tissue was markedly suppressed when compared to control pancreatic explants cultured alone. The effects of the adrenal in maintaining the differentiated acinar component of the pancreas and suppressing media insulin concentration diminished over extended incubation. The addition of adrenals to culture of foetal pancreatic explants after 6 days of control culture (at a time when differentiated acinar cells were not identifiable in the explant) did not result in redifferentiation of the acinar component, but did markedly depress media insulin content. Removal of adrenals after 4 days of co-culture resulted in an immediate rise in media insulin concentration and a rapid decline in pancreatic acinar mass. An adrenal-exocrine pancreatic axis is proposed and it is suggested that foetal adrenal secretions may play an important role in the development of the exocrine pancreas in vivo as well as in vitro."}
{"id": "PMID:1102374", "title": "[The Caceres-USPHS (version HP-3) computer program. Evaluation of automatic analysis of 40,000 ECG (author's transl)].", "content": "The main features of Caceres-USPHS (version HP-3) program for automatic analysis of the ECG are reviewed. Our experience at the Ospedale Regionale di Udine has now reached a total of 40,000 tracings analyzed by such a program and with an HP 1530-A computer system. In a detailed study of 1,000 consecutive computer-analyzed tracings reviewed by three experienced cardiologists, 534 were normal and 466 abnormal. A high degree of specificity was detected in the normal group (only 1.5% of false negatives) while the sensivity was rather low. In the abnormal group there were 18.1% of false positives and while the agreement was 100% in complete bundle branch block and some types of infarction, it was 56% in atrial fibrillation and 47% in incomplete right bundle branch block. Program errors were more common in measurements and pattern recognition; they were unusual in the program logic (0.3%). A significant improvement over comparable data obtained one year previously was attributed to a better quality control in data recording and to increase physician acceptance of the program diagnostic criteria. On the current and past figures of accuracy, it is concluded that the version HP-3 of Caceres-USPHS program represents a step forward in computer-derived ECG interpretation. However more progress is still needed for a larger and more profitable clinical application of this ECG computer program.", "contents": "[The Caceres-USPHS (version HP-3) computer program. Evaluation of automatic analysis of 40,000 ECG (author's transl)]. The main features of Caceres-USPHS (version HP-3) program for automatic analysis of the ECG are reviewed. Our experience at the Ospedale Regionale di Udine has now reached a total of 40,000 tracings analyzed by such a program and with an HP 1530-A computer system. In a detailed study of 1,000 consecutive computer-analyzed tracings reviewed by three experienced cardiologists, 534 were normal and 466 abnormal. A high degree of specificity was detected in the normal group (only 1.5% of false negatives) while the sensivity was rather low. In the abnormal group there were 18.1% of false positives and while the agreement was 100% in complete bundle branch block and some types of infarction, it was 56% in atrial fibrillation and 47% in incomplete right bundle branch block. Program errors were more common in measurements and pattern recognition; they were unusual in the program logic (0.3%). A significant improvement over comparable data obtained one year previously was attributed to a better quality control in data recording and to increase physician acceptance of the program diagnostic criteria. On the current and past figures of accuracy, it is concluded that the version HP-3 of Caceres-USPHS program represents a step forward in computer-derived ECG interpretation. However more progress is still needed for a larger and more profitable clinical application of this ECG computer program."}
{"id": "PMID:1102375", "title": "A new user-oriented electrocardiographic program.", "content": "A new program for electrocardiographic processing will become available in 1975, \"tuned\", as part of a system, to take advantage of newer computer and data acquisition hardware at the same time as past experience on other programs has been carefully retained. User flexibility is a key feature along with safe methodology. The program is undergoing modification as part of a data pool user plan.", "contents": "A new user-oriented electrocardiographic program. A new program for electrocardiographic processing will become available in 1975, \"tuned\", as part of a system, to take advantage of newer computer and data acquisition hardware at the same time as past experience on other programs has been carefully retained. User flexibility is a key feature along with safe methodology. The program is undergoing modification as part of a data pool user plan."}
{"id": "PMID:1102376", "title": "Experiences with different computerprograms for the ECG-analysis.", "content": "As the use of computers for ECG-analysis has left the stage of laboratory investigation, and different systems are offered by industrial firms with high grade promises concerning their reliability, it seemed about time to perform an evaluation of the programs available. Each ECG of 252 adult patients was recorded in one session from adhesive electrodes within 10 minutes on the magnetic tapes of the data-acquisition-units of four different manufacturers. The computer-analysis of each of these ECGs was done by six programs of Pordy (Mt. Sinai-Hospital, New York), Caceres, Smith (Mayo Clinic, Rochester), Arvedson, Riedl with modified criteria of the USPHS and Riedl with criteria of the Minnesota-code. Besides this each ECG was also analysed in the conventional way by each of the three cardiologists involved in this study. The results of the computers' and the physicians' analysis concerning rhythm, extrasystoles, infarctions and ST-T-segment-variations are demonstrated and discussed.", "contents": "Experiences with different computerprograms for the ECG-analysis. As the use of computers for ECG-analysis has left the stage of laboratory investigation, and different systems are offered by industrial firms with high grade promises concerning their reliability, it seemed about time to perform an evaluation of the programs available. Each ECG of 252 adult patients was recorded in one session from adhesive electrodes within 10 minutes on the magnetic tapes of the data-acquisition-units of four different manufacturers. The computer-analysis of each of these ECGs was done by six programs of Pordy (Mt. Sinai-Hospital, New York), Caceres, Smith (Mayo Clinic, Rochester), Arvedson, Riedl with modified criteria of the USPHS and Riedl with criteria of the Minnesota-code. Besides this each ECG was also analysed in the conventional way by each of the three cardiologists involved in this study. The results of the computers' and the physicians' analysis concerning rhythm, extrasystoles, infarctions and ST-T-segment-variations are demonstrated and discussed."}
{"id": "PMID:1102377", "title": "[The use of computers in the exercise of electrocardiography (author's transl)].", "content": "The use of computers in the exercise of electrocardiography is considered. The problems related to this use concern the choice of leads, the filtration of the signal, the recognition of characteristic segments of ECG, the calculation of various indexes and their statistical evaluation for diagnostic purposes. The authors use a bipolar derivation filtered by averaging and digital filters. The characteristic points of ECG are recognized by the analysis of the curvature. The Tchebycheff coefficients of the S-T tract are evaluated; they appeared to be a very good means for judging positive and negative cases.", "contents": "[The use of computers in the exercise of electrocardiography (author's transl)]. The use of computers in the exercise of electrocardiography is considered. The problems related to this use concern the choice of leads, the filtration of the signal, the recognition of characteristic segments of ECG, the calculation of various indexes and their statistical evaluation for diagnostic purposes. The authors use a bipolar derivation filtered by averaging and digital filters. The characteristic points of ECG are recognized by the analysis of the curvature. The Tchebycheff coefficients of the S-T tract are evaluated; they appeared to be a very good means for judging positive and negative cases."}
{"id": "PMID:1102378", "title": "Antitumor activity of polysaccharides from Serratia marcescens.", "content": "A lipopolysaccharide (serratigen) and a polysaccharide (serratimannan) were isolated from Serratia marcescens, red strain No. 51. They were different from any other polysaccharides previously reported. The antitumor activity of these polysaccharides was determined. Serratimannan showed 63% tumor inhibition and serratigen 38%, at a dose of 150 mg/kg, against solid tumor of sarcoma-180 using ICR mice. The fraction obtained by removal of proteins from the crude extract by the Sevag method showed a high antitumor activity against solid tumor of sarcoma-180 using Swiss albino mice, but did not show so high an activity using ICR mice. In total packed cell volume method, these polysaccharides exhibited a high rate of antitumor activity against ascites tumor of sarcoma-180.", "contents": "Antitumor activity of polysaccharides from Serratia marcescens. A lipopolysaccharide (serratigen) and a polysaccharide (serratimannan) were isolated from Serratia marcescens, red strain No. 51. They were different from any other polysaccharides previously reported. The antitumor activity of these polysaccharides was determined. Serratimannan showed 63% tumor inhibition and serratigen 38%, at a dose of 150 mg/kg, against solid tumor of sarcoma-180 using ICR mice. The fraction obtained by removal of proteins from the crude extract by the Sevag method showed a high antitumor activity against solid tumor of sarcoma-180 using Swiss albino mice, but did not show so high an activity using ICR mice. In total packed cell volume method, these polysaccharides exhibited a high rate of antitumor activity against ascites tumor of sarcoma-180."}
{"id": "PMID:1102379", "title": "Immunotherapy of human malignant melanoma with oil-attached BCG cell-wall skeleton.", "content": "A first case of malignant melanoma treated with oil-attached BCG cell-wall skeleton (BCG-CWS) is reported. The patient, a 70-year-old farmer, with metastatic malignant melanoma was treated intralesionally with oil-attached BCG-CWS or was given intradermally with oil-attached BCG-CWS together with irradiated autologous and allogeneic tumor cells. Four months after the start of the immunotherapy with oil-attached BCG-CWS, Primary tumor and metastatic inguinal lymph nodes regressed markedly without any significant complications. Lymphocytosis in the peripheral blood was usually observed after injection of oil-attached BCG-CWS.", "contents": "Immunotherapy of human malignant melanoma with oil-attached BCG cell-wall skeleton. A first case of malignant melanoma treated with oil-attached BCG cell-wall skeleton (BCG-CWS) is reported. The patient, a 70-year-old farmer, with metastatic malignant melanoma was treated intralesionally with oil-attached BCG-CWS or was given intradermally with oil-attached BCG-CWS together with irradiated autologous and allogeneic tumor cells. Four months after the start of the immunotherapy with oil-attached BCG-CWS, Primary tumor and metastatic inguinal lymph nodes regressed markedly without any significant complications. Lymphocytosis in the peripheral blood was usually observed after injection of oil-attached BCG-CWS."}
{"id": "PMID:1102380", "title": "Immunofluorescent staining of gastric mucosal glycoprotein in gastric carcinoma of dogs and rats induced by N-methyl-N'-nitro-N-nitrosoguanidine.", "content": "Immunohistological studies, using the fluorescein isothiocyanate-labeled antibody against gastric mucosal glycoprotein, were made during the development of gastric cancer, induced in dogs and rats by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In an early stage, the regenerative glands were lined by fluorescent mucus cells. Carcinoma cells of orderly glandular structure, produced in dogs, were devoid of fluorescence. Carcinoma cells of less differentiation, produced in rats during further advanced stage, were well fluorescent. The immunofluorescent profiles of such experimentally induced gastric carcinoma were found to be the same as those of human gastric adenocarcinoma.", "contents": "Immunofluorescent staining of gastric mucosal glycoprotein in gastric carcinoma of dogs and rats induced by N-methyl-N'-nitro-N-nitrosoguanidine. Immunohistological studies, using the fluorescein isothiocyanate-labeled antibody against gastric mucosal glycoprotein, were made during the development of gastric cancer, induced in dogs and rats by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). In an early stage, the regenerative glands were lined by fluorescent mucus cells. Carcinoma cells of orderly glandular structure, produced in dogs, were devoid of fluorescence. Carcinoma cells of less differentiation, produced in rats during further advanced stage, were well fluorescent. The immunofluorescent profiles of such experimentally induced gastric carcinoma were found to be the same as those of human gastric adenocarcinoma."}
{"id": "PMID:1102381", "title": "Effect of Nocardia and Mycobacterium cell-wall skeleton on autochthonous tumor grafts.", "content": "Cell-wall skeleton of Nocardia rubra and of Mycobacterium bovis BCG in the oil-attached form, when injected mixes with autografts of spontaneous mammary adenocarcinoma or of methylcholanthrene-induced sarcoma of mice, suppresses the growth of tumor autografts to a demonstrate extent. The BCG whole cell wall showed no effect. The local destruction of tumor autografts did not induce recognizable systemic immunity to the respective tumors. These findings are commended upon from immunological and chemical points of view.", "contents": "Effect of Nocardia and Mycobacterium cell-wall skeleton on autochthonous tumor grafts. Cell-wall skeleton of Nocardia rubra and of Mycobacterium bovis BCG in the oil-attached form, when injected mixes with autografts of spontaneous mammary adenocarcinoma or of methylcholanthrene-induced sarcoma of mice, suppresses the growth of tumor autografts to a demonstrate extent. The BCG whole cell wall showed no effect. The local destruction of tumor autografts did not induce recognizable systemic immunity to the respective tumors. These findings are commended upon from immunological and chemical points of view."}
{"id": "PMID:1102383", "title": "[Iron treatment by effervescent tablets (author's transl)].", "content": "In a randomized double-blind study the new effervescent iron tablet Loesferron was tested in 57 postpartum patients. Hgb and red blood count rose significantly with this treatment. It was found that on effervescent tablet daily was sufficient for a satisfactory result of the treatment. No side effects were noted to this well tasting iron beverage. The reason is probably the prior dilution of the iron compound which delivers lower local iron potentials to the gastric mucosa.", "contents": "[Iron treatment by effervescent tablets (author's transl)]. In a randomized double-blind study the new effervescent iron tablet Loesferron was tested in 57 postpartum patients. Hgb and red blood count rose significantly with this treatment. It was found that on effervescent tablet daily was sufficient for a satisfactory result of the treatment. No side effects were noted to this well tasting iron beverage. The reason is probably the prior dilution of the iron compound which delivers lower local iron potentials to the gastric mucosa."}
{"id": "PMID:1102384", "title": "[Foreign body granulation in the breast after injection of a polyvinylpyrrolidone containing preparation. A case report (author's transl)].", "content": "A case is reported of a patient who developed large foreign body granulations in the breast and in the epigastric area after injection of a polyvinylpyrrolidone containing medication (Depot-Impletol).", "contents": "[Foreign body granulation in the breast after injection of a polyvinylpyrrolidone containing preparation. A case report (author's transl)]. A case is reported of a patient who developed large foreign body granulations in the breast and in the epigastric area after injection of a polyvinylpyrrolidone containing medication (Depot-Impletol)."}
{"id": "PMID:1102394", "title": "Double-blind evaluation of naftidrofuryl in treating elderly confused hospitalised patients.", "content": "In a double-blind comparison between naftidrofuryl and placebo, naftidrofuryl-treated patients improved significantly on scales completed by the nurse, occupational therapist and physician. No improvement was found for placbo-treated patients. When the response to each treatment was compared, naftidrofuryl was found to be significantly better than placebo on both nurse and physician assessment. There was considerable variation between assessors which suggests that they were measuring different aspects of the patients' condition. There was also considerable variation in patient response, some improved on naftidrofuryl and some deteriorated; some improved on placebo and some deteriorated. However, overall it would seem that patients admitted to hospital with confusional states benefit from treatment with naftidrofuryl.", "contents": "Double-blind evaluation of naftidrofuryl in treating elderly confused hospitalised patients. In a double-blind comparison between naftidrofuryl and placebo, naftidrofuryl-treated patients improved significantly on scales completed by the nurse, occupational therapist and physician. No improvement was found for placbo-treated patients. When the response to each treatment was compared, naftidrofuryl was found to be significantly better than placebo on both nurse and physician assessment. There was considerable variation between assessors which suggests that they were measuring different aspects of the patients' condition. There was also considerable variation in patient response, some improved on naftidrofuryl and some deteriorated; some improved on placebo and some deteriorated. However, overall it would seem that patients admitted to hospital with confusional states benefit from treatment with naftidrofuryl."}
{"id": "PMID:1102395", "title": "Immune function and survival in a long-lived mouse strain subjected to undernutrition.", "content": "Functional immune changes were monitored in populations of the long-lived C57BL/6J strain of mice which were subjected to dietary restriction from time of weaning or subjected to such restriction both before and after weaning, along with the appropriate control populations. Responses to T and B cell mitogens (PHA, Con-A, pokeweed, bacterial lipopolysaccharide, and PPD), to injected sheep red blood cells, and measurement of skin allograft rejection rates were followed. Early in life, restricted mice appear immunosuppressed, as judged by all these parameters. Skin allograft rejection remained suppressed until relatively late in life. Other responses tended to reverse from the earlier pattern; by mid-life restricted mice responded better than controls. Dietary restriction profoundly affects the immune system. Mice on such regimes display anatomic and certain immune functional changes which suggest that the immune system may mature less rapidly and stay \"younger\" longer than in the controls. Furthermore, dietary restriction results in prolongation of life span.", "contents": "Immune function and survival in a long-lived mouse strain subjected to undernutrition. Functional immune changes were monitored in populations of the long-lived C57BL/6J strain of mice which were subjected to dietary restriction from time of weaning or subjected to such restriction both before and after weaning, along with the appropriate control populations. Responses to T and B cell mitogens (PHA, Con-A, pokeweed, bacterial lipopolysaccharide, and PPD), to injected sheep red blood cells, and measurement of skin allograft rejection rates were followed. Early in life, restricted mice appear immunosuppressed, as judged by all these parameters. Skin allograft rejection remained suppressed until relatively late in life. Other responses tended to reverse from the earlier pattern; by mid-life restricted mice responded better than controls. Dietary restriction profoundly affects the immune system. Mice on such regimes display anatomic and certain immune functional changes which suggest that the immune system may mature less rapidly and stay \"younger\" longer than in the controls. Furthermore, dietary restriction results in prolongation of life span."}
{"id": "PMID:1102397", "title": "Reticulin antibodies in patients with coeliac disease and their relatives.", "content": "The sera of 69 index coeliac patients, 121 of their first-degree relatives, and 104 controls were screened for the presence of reticulin antibodies. Among the untreated coeliac patients 75% of adults and 93% of children had reticulin antibody in their serum. Reticulin antibody was not present in any adequately treated coeliac patient. Of the first-degree relatives, 21 were reticulin antibody positive; 17 of these were biopsied and 12 were shown to have coeliac disease. Sixty-five of the coeliac relatives who did not have reticulin antibodies in their sera were biopsied and two had coeliac disease. Of the 68 relatives and 63 controls with normal biopsies, five of the relatives and four of the controls were reticulin antibody positive.", "contents": "Reticulin antibodies in patients with coeliac disease and their relatives. The sera of 69 index coeliac patients, 121 of their first-degree relatives, and 104 controls were screened for the presence of reticulin antibodies. Among the untreated coeliac patients 75% of adults and 93% of children had reticulin antibody in their serum. Reticulin antibody was not present in any adequately treated coeliac patient. Of the first-degree relatives, 21 were reticulin antibody positive; 17 of these were biopsied and 12 were shown to have coeliac disease. Sixty-five of the coeliac relatives who did not have reticulin antibodies in their sera were biopsied and two had coeliac disease. Of the 68 relatives and 63 controls with normal biopsies, five of the relatives and four of the controls were reticulin antibody positive."}
{"id": "PMID:1102398", "title": "Early changes in coagulation following a paracetamol overdose and a controlled trial of fresh frozen plasma therapy.", "content": "Early changes in coagulation were found in patients following a paracetamol overdose. Low levels of clotting factors II, V and VII were present within 24 hours of the overdose. As the levels of factor II correlated with plasma fibrinogen values at this time, it is possible that they were consumed in the process of intravascular coagulation, although this was not supported by the presence of raised titres of fibrin degradation products. The prothrombin time ratio was greater than 2-2 within 30 hours of ingestion of the overdose in all patients who eventually died, whereas it was less than this in those developing only moderate liver damage. The administration of fresh frozen plasma to patients did appear to reduce the maximum abnormality of the prothrombin time ratio, which was significantly less three days after the overdose in the group receiving fresh frozen plasma. However, the coagulation disturbance was of short duration, and the prothrombin time ratio had also returned to normal within one week of the overdose in the control patients, and the administration of fresh frozen plasma did not appear to reduce the morbidity or mortality in the treated patients.", "contents": "Early changes in coagulation following a paracetamol overdose and a controlled trial of fresh frozen plasma therapy. Early changes in coagulation were found in patients following a paracetamol overdose. Low levels of clotting factors II, V and VII were present within 24 hours of the overdose. As the levels of factor II correlated with plasma fibrinogen values at this time, it is possible that they were consumed in the process of intravascular coagulation, although this was not supported by the presence of raised titres of fibrin degradation products. The prothrombin time ratio was greater than 2-2 within 30 hours of ingestion of the overdose in all patients who eventually died, whereas it was less than this in those developing only moderate liver damage. The administration of fresh frozen plasma to patients did appear to reduce the maximum abnormality of the prothrombin time ratio, which was significantly less three days after the overdose in the group receiving fresh frozen plasma. However, the coagulation disturbance was of short duration, and the prothrombin time ratio had also returned to normal within one week of the overdose in the control patients, and the administration of fresh frozen plasma did not appear to reduce the morbidity or mortality in the treated patients."}
{"id": "PMID:1102399", "title": "The use of fresh frozen plasma or a concentrate of factor IX as replacement therapy before liver biopsy.", "content": "Thirty patients with various types of chronic liver disease and a prothrombin time prolonged for four or more seconds who required needle liver biopsy for diagnostic purposes were given either fresh frozen plasma or a concentrate of clotting factors as a prophylactic measure. The prothrombin time returned to within normal limits in seven of the 15 patients given the concentrate and in three of those receiving fresh frozen plasma. Levels of factors II, IX, and X showed increases of about 30% following concentrate administration; corresponding rises in the group given fresh frozen plasma were less. This was because of the smaller quantity of clotting factors administered with fresh frozen plasma and the increase in factor II and IX activity/kg body weight/unit of clotting factor injected was greater when fresh frozen plasma was used. In neither group was there clinical evidence of bleeding, but it was of interest that most of the clotting factor levels, except in factor II, before biopsy were above those normally required for haemostasis. No evidence of disseminated intravascular coagulation was found with the concentrate injection, and the most worrying finding was the appearance of HBAg some months later in three patients, two from the concentrate group and one from those given fresh frozen plasma. However, the conversion of these patients to HBAg positive may be unrelated to the clotting factor replacement therapy.", "contents": "The use of fresh frozen plasma or a concentrate of factor IX as replacement therapy before liver biopsy. Thirty patients with various types of chronic liver disease and a prothrombin time prolonged for four or more seconds who required needle liver biopsy for diagnostic purposes were given either fresh frozen plasma or a concentrate of clotting factors as a prophylactic measure. The prothrombin time returned to within normal limits in seven of the 15 patients given the concentrate and in three of those receiving fresh frozen plasma. Levels of factors II, IX, and X showed increases of about 30% following concentrate administration; corresponding rises in the group given fresh frozen plasma were less. This was because of the smaller quantity of clotting factors administered with fresh frozen plasma and the increase in factor II and IX activity/kg body weight/unit of clotting factor injected was greater when fresh frozen plasma was used. In neither group was there clinical evidence of bleeding, but it was of interest that most of the clotting factor levels, except in factor II, before biopsy were above those normally required for haemostasis. No evidence of disseminated intravascular coagulation was found with the concentrate injection, and the most worrying finding was the appearance of HBAg some months later in three patients, two from the concentrate group and one from those given fresh frozen plasma. However, the conversion of these patients to HBAg positive may be unrelated to the clotting factor replacement therapy."}
{"id": "PMID:1102400", "title": "Function of the anal sphincters in spinal man.", "content": "Eight patients with traumatic transverse lesions of the spinal cord and eight healthy controls were examined with respect to the function of the anal sphincters. Continuous recordings of anal pressure and electromyograms from the striated sphincter muscles were obtained during gradual rectal distension by means of an air-filled balloon. The patients were more prone than the controls to display a cessation of all electrical activity from the striated muscles in response to rectal distension and they also exhibited a less pronounced inflation reflex. It is concluded that upon rectal distension in normal man the striated muscles are stimulated by a cerebral centre at an unconscious level as well as by a low centre of the spinal cord. The former accounts for an augmented inflation reflex and maintained or increased electrical activity when rectal distension is substantial. The smooth internal sphincter was found to be independent of cerebral influence at rest as well as during rectal distension.", "contents": "Function of the anal sphincters in spinal man. Eight patients with traumatic transverse lesions of the spinal cord and eight healthy controls were examined with respect to the function of the anal sphincters. Continuous recordings of anal pressure and electromyograms from the striated sphincter muscles were obtained during gradual rectal distension by means of an air-filled balloon. The patients were more prone than the controls to display a cessation of all electrical activity from the striated muscles in response to rectal distension and they also exhibited a less pronounced inflation reflex. It is concluded that upon rectal distension in normal man the striated muscles are stimulated by a cerebral centre at an unconscious level as well as by a low centre of the spinal cord. The former accounts for an augmented inflation reflex and maintained or increased electrical activity when rectal distension is substantial. The smooth internal sphincter was found to be independent of cerebral influence at rest as well as during rectal distension."}
{"id": "PMID:1102401", "title": "Aspiration biopsy of carcinoma of the pancreas.", "content": "Peroperative pancreatic aspiration biopsies were performed on 21 patients with pancreatic lesions using a standard 20-ml disposable syringe and a 21-gauge needle. No complications were recorded which could be attributed to this procedure. A further 10 aspiration biopsies were carried out on postmortem specimens in an attempt to determine the accuracy of this method in the diagnosis of carcinoma of the pancreas. It is concluded that peroperative pancreatic needle biopsy is a safe procedure which is easily performed without special instruments. In can be of enormous value to the surgeon in planning the treatment of patients with pancreatic lesions. In cases where there is an operable mass in the pancreas it offers a simple and quick method of determining the presence of malignant cells and thus definitive surgery may be performed with confidence. For the inoperable cases it offers a method of histological confirmation of the operative findings.", "contents": "Aspiration biopsy of carcinoma of the pancreas. Peroperative pancreatic aspiration biopsies were performed on 21 patients with pancreatic lesions using a standard 20-ml disposable syringe and a 21-gauge needle. No complications were recorded which could be attributed to this procedure. A further 10 aspiration biopsies were carried out on postmortem specimens in an attempt to determine the accuracy of this method in the diagnosis of carcinoma of the pancreas. It is concluded that peroperative pancreatic needle biopsy is a safe procedure which is easily performed without special instruments. In can be of enormous value to the surgeon in planning the treatment of patients with pancreatic lesions. In cases where there is an operable mass in the pancreas it offers a simple and quick method of determining the presence of malignant cells and thus definitive surgery may be performed with confidence. For the inoperable cases it offers a method of histological confirmation of the operative findings."}
{"id": "PMID:1102406", "title": "[Action mechanism of sodium polyacrylates against stomach ulcer].", "content": "Three sodium polyacrylates (PAS) with different viscosities were examined for antiulcerogenic properties. Rats were fed with sugar containing a PAS for 4 hr and subjected to 12 hr reserpinization or stress by water immersion. Lower incidence of ulceration and larger amount of stomach content after the experiment were found in the PAS administered groups. The higher viscous PAS was more effective in prevention of ulceration and in prolongation of gastric emptying. Intragastric administration of the drugs to pylorus ligated rats reduced forestomach ulceration. However, differences in the antiulcerogenic activity of three drugs were not observed. The effects on the gastric secretion of three PAS (50 mg) were also examined, using pylorus ligated (6 hr) rats. In PAS groups, the free acid in the gastric juice was reduced to some extent. There was, however, no relation between the antiacid effect and viscosity. The drugs inhibited the peptic digestion in vitro, but the difference of viscosity was not related to the antipeptic effect. It is concluded that prolongation of gastric emptying may be a major factor in the antiulcerogenic activity of PAS.", "contents": "[Action mechanism of sodium polyacrylates against stomach ulcer]. Three sodium polyacrylates (PAS) with different viscosities were examined for antiulcerogenic properties. Rats were fed with sugar containing a PAS for 4 hr and subjected to 12 hr reserpinization or stress by water immersion. Lower incidence of ulceration and larger amount of stomach content after the experiment were found in the PAS administered groups. The higher viscous PAS was more effective in prevention of ulceration and in prolongation of gastric emptying. Intragastric administration of the drugs to pylorus ligated rats reduced forestomach ulceration. However, differences in the antiulcerogenic activity of three drugs were not observed. The effects on the gastric secretion of three PAS (50 mg) were also examined, using pylorus ligated (6 hr) rats. In PAS groups, the free acid in the gastric juice was reduced to some extent. There was, however, no relation between the antiacid effect and viscosity. The drugs inhibited the peptic digestion in vitro, but the difference of viscosity was not related to the antipeptic effect. It is concluded that prolongation of gastric emptying may be a major factor in the antiulcerogenic activity of PAS."}
{"id": "PMID:1102408", "title": "The effect of fatty acids and ketone bodies on the biosynthesis of insulin in isolated pancreatic islets of obese hyperglycemic mice.", "content": "Collagenase-isolated islets from obese hyperglycemic mice were used to study the effects of fatty acids and ketone bodies on insulin biosynthesis. The biosynthetic rate was quantitated as the incorporation of 3H-leucine into proinsulin and insulin, which were separated from the crude islet homogenates with anti-insulin serum coupled to cyanogen bromide activated Sepharose 4B. Palmitate and oleate did not stimulate the incorporation into proinsulin and insulin while 16.7 mM glucose caused a nine-fold increase of 3H-leucine incorporation during a 3 h incubation as compared to the basal buffer alone. A weak stimulatory effect was seen with 5 mM octanoate. The ketone bodies had a weak stimulating effect on islet protein biosynthesis, but no preferential stimulus to the insulin biosynthesis was found.", "contents": "The effect of fatty acids and ketone bodies on the biosynthesis of insulin in isolated pancreatic islets of obese hyperglycemic mice. Collagenase-isolated islets from obese hyperglycemic mice were used to study the effects of fatty acids and ketone bodies on insulin biosynthesis. The biosynthetic rate was quantitated as the incorporation of 3H-leucine into proinsulin and insulin, which were separated from the crude islet homogenates with anti-insulin serum coupled to cyanogen bromide activated Sepharose 4B. Palmitate and oleate did not stimulate the incorporation into proinsulin and insulin while 16.7 mM glucose caused a nine-fold increase of 3H-leucine incorporation during a 3 h incubation as compared to the basal buffer alone. A weak stimulatory effect was seen with 5 mM octanoate. The ketone bodies had a weak stimulating effect on islet protein biosynthesis, but no preferential stimulus to the insulin biosynthesis was found."}
{"id": "PMID:1102409", "title": "Evidence for the D-cell of the pancreas secreting somatostatin.", "content": "By immunofluorescence, somatostatin-, glucagon- and insulin-containing cells are localized in serial sections of the pigeon pancreas. The distribution of the somatostatin immunofluorescent-cells corresponds to that of the D-cells (A1-cells), which are particularly numerous in this animal species. This observation, coupled with the finding of D, A and B-cells at the ultrastructural level, indicates that the D-cell is responsible for the secretion of somatostatin.", "contents": "Evidence for the D-cell of the pancreas secreting somatostatin. By immunofluorescence, somatostatin-, glucagon- and insulin-containing cells are localized in serial sections of the pigeon pancreas. The distribution of the somatostatin immunofluorescent-cells corresponds to that of the D-cells (A1-cells), which are particularly numerous in this animal species. This observation, coupled with the finding of D, A and B-cells at the ultrastructural level, indicates that the D-cell is responsible for the secretion of somatostatin."}
{"id": "PMID:1102411", "title": "A naturally occurring Cu-thionein in Saccharomyces cerevisiae.", "content": "A naturally occurring monodisperse Cu-thionein was prepared using ammonium sulfate precipitation followed by ion exchange (DEAE 23) and gel chromatography (Sephadex G-75). The chromatographic steps were repeated at least twice, or until the Cu-thionein remained homogeneous when subjected to analytical polyacrylamide disc electrophoresis. The molecular weight of this copper protein was 9500+/-500. Up to 24.3% cysteine residues were determined, indicating the relationship to the metallothioneins. Aromatic amino acids were virtually absent, while there were about three times as many acidic amino acid residues, including aspartate and glutamate, as in metallothioneins. 10 g atoms of Cu were measured per mole of protein. The copper binding strength of thionein was extremely high. Displacement by protons (pH 1.5) and gel chromatography or dialysis employing EDTA were not effective. Dialysis against diethyldithiocarbamate produced a protein essentially free of copper. Both the ultraviolet properties and the circular dichroism measurements proved identical with those properties reported for artificially prepared Cu-thionein (see ref.[1]. The major absorption was in the far ultraviolet region with a weak shoulder at 270 nm attributable to copper charge-transfer transititions. 6 Cotton extrema were seen at 213, 283 and 302 nm (negative) and 245, 328 and 359 nm (positive). The possible role of Cu-thionein as an electron transport system was discussed.", "contents": "A naturally occurring Cu-thionein in Saccharomyces cerevisiae. A naturally occurring monodisperse Cu-thionein was prepared using ammonium sulfate precipitation followed by ion exchange (DEAE 23) and gel chromatography (Sephadex G-75). The chromatographic steps were repeated at least twice, or until the Cu-thionein remained homogeneous when subjected to analytical polyacrylamide disc electrophoresis. The molecular weight of this copper protein was 9500+/-500. Up to 24.3% cysteine residues were determined, indicating the relationship to the metallothioneins. Aromatic amino acids were virtually absent, while there were about three times as many acidic amino acid residues, including aspartate and glutamate, as in metallothioneins. 10 g atoms of Cu were measured per mole of protein. The copper binding strength of thionein was extremely high. Displacement by protons (pH 1.5) and gel chromatography or dialysis employing EDTA were not effective. Dialysis against diethyldithiocarbamate produced a protein essentially free of copper. Both the ultraviolet properties and the circular dichroism measurements proved identical with those properties reported for artificially prepared Cu-thionein (see ref.[1]. The major absorption was in the far ultraviolet region with a weak shoulder at 270 nm attributable to copper charge-transfer transititions. 6 Cotton extrema were seen at 213, 283 and 302 nm (negative) and 245, 328 and 359 nm (positive). The possible role of Cu-thionein as an electron transport system was discussed."}
{"id": "PMID:1102412", "title": "Metabolic transformation of colchicine, III. Inhibition of phosphatases by the metabolite O10-demethylcolchicine (colchiceine) and their reactivation by divalent cations.", "content": "Colchicine and some of its metabolites and derivatives were investigated regarding their inhibitory action against alkaline phosphatases from E. coli and calf intestine, and against acid phosphatase from potato. Colchicine itself was essentially ineffective, also its metabolites O2-demethyl- and O3-demethylcolchicine. The metabolite O10-demethylcolchicine (= colchiceine) was found to be a powerful, non-competitive inhibitor for all three phosphatases with Ki in the range of 0.45-6.0mM. Amongst the derivatives of colchicine, colchiceyltaurine proved to be a non-competitive inhibitor of acid phosphatase (Ki=3.0mM); desacetylcolchiceine behaved like colchiceine. The inhbitory actions of colchiceine and desacetylcholchiceine could be completely reversed by the addition of Mg2, Ca2, or Zn2, respectively. This interaction with divalent cations demonstrates a novel type of action of the metabolite colchiceine when compared with its mother alkaloid and is possibly of major biological significance.", "contents": "Metabolic transformation of colchicine, III. Inhibition of phosphatases by the metabolite O10-demethylcolchicine (colchiceine) and their reactivation by divalent cations. Colchicine and some of its metabolites and derivatives were investigated regarding their inhibitory action against alkaline phosphatases from E. coli and calf intestine, and against acid phosphatase from potato. Colchicine itself was essentially ineffective, also its metabolites O2-demethyl- and O3-demethylcolchicine. The metabolite O10-demethylcolchicine (= colchiceine) was found to be a powerful, non-competitive inhibitor for all three phosphatases with Ki in the range of 0.45-6.0mM. Amongst the derivatives of colchicine, colchiceyltaurine proved to be a non-competitive inhibitor of acid phosphatase (Ki=3.0mM); desacetylcolchiceine behaved like colchiceine. The inhbitory actions of colchiceine and desacetylcholchiceine could be completely reversed by the addition of Mg2, Ca2, or Zn2, respectively. This interaction with divalent cations demonstrates a novel type of action of the metabolite colchiceine when compared with its mother alkaloid and is possibly of major biological significance."}
{"id": "PMID:1102413", "title": "Interactions of concanavalin A with isolated pancreatic islets.", "content": "Pancreatic islets of mice were isolated by the collagenase method. After a preincubation period of 20 min they were incubated in Krebs-Ringer-albumin buffer in the presence of glucose. Concanavalin A (2 mg/ml) inhibits the glucose-induced release of insulin; this same property is shown by Concanavalin A bound to nonphagocytosable beads of Sephrose at lower concentrations (0.4 mg/ml). In pancreatic islets stimulated by tolbutamide, glibenclamide or arginine the secretion of insulin is not inhibited in the presence of Concanavalin A. The incorporation of tritiated leucine is not influenced by Concanavalin A. Since desialized pancreatic islets react in the same way the glucose-induced secretion of insulin appears to be a membrane-dependent process.", "contents": "Interactions of concanavalin A with isolated pancreatic islets. Pancreatic islets of mice were isolated by the collagenase method. After a preincubation period of 20 min they were incubated in Krebs-Ringer-albumin buffer in the presence of glucose. Concanavalin A (2 mg/ml) inhibits the glucose-induced release of insulin; this same property is shown by Concanavalin A bound to nonphagocytosable beads of Sephrose at lower concentrations (0.4 mg/ml). In pancreatic islets stimulated by tolbutamide, glibenclamide or arginine the secretion of insulin is not inhibited in the presence of Concanavalin A. The incorporation of tritiated leucine is not influenced by Concanavalin A. Since desialized pancreatic islets react in the same way the glucose-induced secretion of insulin appears to be a membrane-dependent process."}
{"id": "PMID:1102426", "title": "Thyroglossal duct carcinoma.", "content": "Primary carcinoma of the thyroglossal duct is rare. This discussion reports two cases and reviews the 50 previously reported in the literature. The criteria for diagnosis include evidence of a thyroglossal duct remnant and a normal thyroid gland. The differentiation from cystic metastases to lymph nodes is pointed out. The histologic types parallel those of carcinoma of the thyroid gland, papillary carcinoma being the most common and having a generally favorable prognosis. The clinical presentation of these tumors is similar to that with benign cysts and thus is of limited value in the diagnosis.", "contents": "Thyroglossal duct carcinoma. Primary carcinoma of the thyroglossal duct is rare. This discussion reports two cases and reviews the 50 previously reported in the literature. The criteria for diagnosis include evidence of a thyroglossal duct remnant and a normal thyroid gland. The differentiation from cystic metastases to lymph nodes is pointed out. The histologic types parallel those of carcinoma of the thyroid gland, papillary carcinoma being the most common and having a generally favorable prognosis. The clinical presentation of these tumors is similar to that with benign cysts and thus is of limited value in the diagnosis."}
{"id": "PMID:1102427", "title": "Recent developments in foetal haemoglobin research.", "content": "There have been numerous new contributions to the knowledge of foetal haemoglobin over the last few years. It is, therefore, timely to review them together. They throw light on the arrangement on the chromosome of non-alpha chain genes, and on the condition generally known as Hereditary Persistence of Foetal Haemoglobin (HPFH) and have contributed to other aspects of human ontogeny and physiology.", "contents": "Recent developments in foetal haemoglobin research. There have been numerous new contributions to the knowledge of foetal haemoglobin over the last few years. It is, therefore, timely to review them together. They throw light on the arrangement on the chromosome of non-alpha chain genes, and on the condition generally known as Hereditary Persistence of Foetal Haemoglobin (HPFH) and have contributed to other aspects of human ontogeny and physiology."}
{"id": "PMID:1102428", "title": "Apparent \"in situ\" clone of cytogenetically marked ataxia-telangiectasia lymphocytes.", "content": "Six adjacent metaphases, each with the same cytogenetic aberration of a group D chromosome, most probably a No. 14, were observed in a field of a slide from a 96-hour culture of lymphocytes from an individual with ataxia-telangiectasia (AT). None of the 304 other metaphases examined from this or other simultaneous cultures of this individual showed such an aberration. It seems most likely that an \"in situ\" marked clone has been observed and this supports interpretation of consistent cytogenetic abnormalities in those with AT as having clonal origin. The method of slide preparation employed which involves placing, rather than dropping, the cell suspension on the slide may facilitate detection of \"in situ\" clones.", "contents": "Apparent \"in situ\" clone of cytogenetically marked ataxia-telangiectasia lymphocytes. Six adjacent metaphases, each with the same cytogenetic aberration of a group D chromosome, most probably a No. 14, were observed in a field of a slide from a 96-hour culture of lymphocytes from an individual with ataxia-telangiectasia (AT). None of the 304 other metaphases examined from this or other simultaneous cultures of this individual showed such an aberration. It seems most likely that an \"in situ\" marked clone has been observed and this supports interpretation of consistent cytogenetic abnormalities in those with AT as having clonal origin. The method of slide preparation employed which involves placing, rather than dropping, the cell suspension on the slide may facilitate detection of \"in situ\" clones."}
{"id": "PMID:1102436", "title": "[Possibilities of a quality control in the diagnosis of tuberculosis (author's transl)].", "content": "A method is described which allows a quality control for demonstrating mycobacteria in the diangosis of tuberculosis. It does not test the differentiating procedures, but the manner of culturing and concentrating the bacteria by applying a statistical evaluation of serial experiments, thus allowing the statistically proven determination of the bacterial count. For this an evaluation method given by Cavalli-Storza was employed.", "contents": "[Possibilities of a quality control in the diagnosis of tuberculosis (author's transl)]. A method is described which allows a quality control for demonstrating mycobacteria in the diangosis of tuberculosis. It does not test the differentiating procedures, but the manner of culturing and concentrating the bacteria by applying a statistical evaluation of serial experiments, thus allowing the statistically proven determination of the bacterial count. For this an evaluation method given by Cavalli-Storza was employed."}
{"id": "PMID:1102437", "title": "[Cellular immune reaction in acute and chronic viral hepatitis (author's transl)].", "content": "The course of viral hepatitis is markedly influenced by the immunologic response to the infective agent. The immune defense of viral diseases is specially connected to an intact function of T lymphocytes. Investigation of T cell function during acute and chronic viral hepatitis showed an altered immune response, recognizable from the relative number of T lymphocytes, the affinity and ability of lymphocytes to be stimulated by phytohemagglutinin and the demonstration of in vivo activated lymphocytes. Although the interpretation of some of these findings is still difficult, the study of the various cellular immune reactions permits a better understanding of the pathogenesis and course of viral hepatitis.", "contents": "[Cellular immune reaction in acute and chronic viral hepatitis (author's transl)]. The course of viral hepatitis is markedly influenced by the immunologic response to the infective agent. The immune defense of viral diseases is specially connected to an intact function of T lymphocytes. Investigation of T cell function during acute and chronic viral hepatitis showed an altered immune response, recognizable from the relative number of T lymphocytes, the affinity and ability of lymphocytes to be stimulated by phytohemagglutinin and the demonstration of in vivo activated lymphocytes. Although the interpretation of some of these findings is still difficult, the study of the various cellular immune reactions permits a better understanding of the pathogenesis and course of viral hepatitis."}
{"id": "PMID:1102444", "title": "The potentiality of antibody-producing cells. I Bispecific cell occurrence in double stimulated cultures of syngeneic or allogeneic spleen cells of the mouse.", "content": "Cultures of spleen cells from Swiss, C57Bl or DBA/2 mice stimulated with 2,4,6-trinitrophenyl (TNP) conjugated sheep erythrocytes (SRBC) were used for studying the in vitro responses to TNP and native SRBC antigens and the frequency of occurrence of cells responding to both antigens. The response was revealed by plating the cultured cells with both native SRBC and TNP-conjugated pigeon erythrocytes (TNP--PRBC). Specific responses were obtained in all the cultures. Bispecific haemolytic plaque-forming cells (PFC) were detected in almost all cultures of individual Swiss mice cells with a frequency of 2-5--14 PFC/10(6) cells recovered. In DBA/2 cell cultures bispecific PFC were found in half the cultures (2-5--8-3/10(6) cells) and in C57Bl cell cultures in 30 per cent of the cultures (7--21/10(6) cells). When cells from individual Swiss mice immunized in vivo with TNP--SRBC were as an allogeneic culture from the 2nd day after immunization in the presence of TNP--SRBC, the frequency of bispecific PFC increased from 8 to 30/10(6) cells. Mixed allogeneic cultures of normal C57Bl and DBA/2 cells yielded high specific responses with regular occurrence of bispecific PFC only when the numbers of cells cultured together was small. However, when allogeneic cells were mixed 24 hours after starting the cultures, all responses were stimulated and bispecific PFC were found in considerable numbers (4--33/10(6) cells). Cross-reactivity between TNP--PRBC and native SRBC antigens was studied by culturing cells with each of the antigens and plating the cells with both, or by immunizing in vivo with SRBC or PRBC and culturing the cells with both antigens from the 2nd to the 5th day after immunization with both antigens. In no instance did bispecific PFC exceed background levels (0-1--0-6/10(6) cells) in these control experiments.", "contents": "The potentiality of antibody-producing cells. I Bispecific cell occurrence in double stimulated cultures of syngeneic or allogeneic spleen cells of the mouse. Cultures of spleen cells from Swiss, C57Bl or DBA/2 mice stimulated with 2,4,6-trinitrophenyl (TNP) conjugated sheep erythrocytes (SRBC) were used for studying the in vitro responses to TNP and native SRBC antigens and the frequency of occurrence of cells responding to both antigens. The response was revealed by plating the cultured cells with both native SRBC and TNP-conjugated pigeon erythrocytes (TNP--PRBC). Specific responses were obtained in all the cultures. Bispecific haemolytic plaque-forming cells (PFC) were detected in almost all cultures of individual Swiss mice cells with a frequency of 2-5--14 PFC/10(6) cells recovered. In DBA/2 cell cultures bispecific PFC were found in half the cultures (2-5--8-3/10(6) cells) and in C57Bl cell cultures in 30 per cent of the cultures (7--21/10(6) cells). When cells from individual Swiss mice immunized in vivo with TNP--SRBC were as an allogeneic culture from the 2nd day after immunization in the presence of TNP--SRBC, the frequency of bispecific PFC increased from 8 to 30/10(6) cells. Mixed allogeneic cultures of normal C57Bl and DBA/2 cells yielded high specific responses with regular occurrence of bispecific PFC only when the numbers of cells cultured together was small. However, when allogeneic cells were mixed 24 hours after starting the cultures, all responses were stimulated and bispecific PFC were found in considerable numbers (4--33/10(6) cells). Cross-reactivity between TNP--PRBC and native SRBC antigens was studied by culturing cells with each of the antigens and plating the cells with both, or by immunizing in vivo with SRBC or PRBC and culturing the cells with both antigens from the 2nd to the 5th day after immunization with both antigens. In no instance did bispecific PFC exceed background levels (0-1--0-6/10(6) cells) in these control experiments."}
{"id": "PMID:1102445", "title": "DNA synthesis in rabbit spleen explants. Its relationship to cell division.", "content": "The synthesis of DNA, as measured by the incorporation of [3H]-thymidine into the nuclei of cells of cultured explants of rabbit spleen, has been studied and correlated with estimation of DNA content of these nuclei using Feulgen densitometry, with and without colcemid arrest. Observations of the proportion of nuclei labelled with [3H]thymidine and their DNA content indicated that many cells were incorporating this precursor for DNA synthesis without the concomitant increases in DNA expected of cells in division cycle. Colcemid arrest failed to produce the changes in DNA content expected of a cell population as rapidly dividing as the labelling kinetics suggested. The results indicated that [3H]thymidine-labelled material, possibly DNA, was being transferred between cells and this accounted for the increased labelling index seen after pulsing with [3H]thymidine. The suppression of [3H]thymidine uptake by hydroxyurea indicated that this was not repair synthesis of damaged DNA.", "contents": "DNA synthesis in rabbit spleen explants. Its relationship to cell division. The synthesis of DNA, as measured by the incorporation of [3H]-thymidine into the nuclei of cells of cultured explants of rabbit spleen, has been studied and correlated with estimation of DNA content of these nuclei using Feulgen densitometry, with and without colcemid arrest. Observations of the proportion of nuclei labelled with [3H]thymidine and their DNA content indicated that many cells were incorporating this precursor for DNA synthesis without the concomitant increases in DNA expected of cells in division cycle. Colcemid arrest failed to produce the changes in DNA content expected of a cell population as rapidly dividing as the labelling kinetics suggested. The results indicated that [3H]thymidine-labelled material, possibly DNA, was being transferred between cells and this accounted for the increased labelling index seen after pulsing with [3H]thymidine. The suppression of [3H]thymidine uptake by hydroxyurea indicated that this was not repair synthesis of damaged DNA."}
{"id": "PMID:1102446", "title": "Effects of mitogens for mouse B lymphocytes on chicken lymphoid cells.", "content": "Lipopolysaccharide from E. coli (LPS) and purified protein derivative from tuberculin (PPD) increased the [3H]thymidine incorporation of chicken spleen cells in culture. No such stimulation was observed with dextran sulphate. Thymus and bursa lymphocytes were not stimulated by any of these compounds. Spleen cells from chickens chemically bursectomized with cyclophosphamide treatment showed decreased responses to LPS and PPD, but responded normally to the T-cell mitogen concanavalin A. None of the tested mitogens induced polyclonal antibody synthesis or directly enhanced the primary antibody response to sheep erythrocytes (SRBC) in spleen cell cultures. LPS-coated SRBC, however, enhanced the in vitro antibody response to SRBC. The results suggest a moderate proliferative response of chicken lymphocytes to LPS and PPD, possibly involving B cells, but not further effects comparable to those on mouse B lymphocytes.", "contents": "Effects of mitogens for mouse B lymphocytes on chicken lymphoid cells. Lipopolysaccharide from E. coli (LPS) and purified protein derivative from tuberculin (PPD) increased the [3H]thymidine incorporation of chicken spleen cells in culture. No such stimulation was observed with dextran sulphate. Thymus and bursa lymphocytes were not stimulated by any of these compounds. Spleen cells from chickens chemically bursectomized with cyclophosphamide treatment showed decreased responses to LPS and PPD, but responded normally to the T-cell mitogen concanavalin A. None of the tested mitogens induced polyclonal antibody synthesis or directly enhanced the primary antibody response to sheep erythrocytes (SRBC) in spleen cell cultures. LPS-coated SRBC, however, enhanced the in vitro antibody response to SRBC. The results suggest a moderate proliferative response of chicken lymphocytes to LPS and PPD, possibly involving B cells, but not further effects comparable to those on mouse B lymphocytes."}
{"id": "PMID:1102447", "title": "Non-specific and DNP--erythrocyte rosette formation after contact sensitization with DNCB.", "content": "DNA--guinea-pig erythrocyte rosette-forming cells (RFC), as found after contact sensitization of guinea-pigs with DNCB, were shown to be highly sensitive to pretreatment with cyclophosphamide or X-irradiation, which suggests that these cells are B cells. The finding was confirmed by rosette-blocking experiments using an anti-immunoglobulin serum. It has already been shown that they are not directly related to antibody production. Possibly they represent precursors of antibody-producing cells. An increase in rosette formation, using non-antigenically related erythrocytes, was shown to parallel development of cell-mediated immunity (CMI) in the lymph nodes. These non-specifically induced RFC were also found to be B cells. Data were obtained which make it improbable that either proliferation or influx of B cells during development of CMI plays a major role in this increase. An alternative mechanism is suggested, while a possible role for these non-specifically induced RC is proposed.", "contents": "Non-specific and DNP--erythrocyte rosette formation after contact sensitization with DNCB. DNA--guinea-pig erythrocyte rosette-forming cells (RFC), as found after contact sensitization of guinea-pigs with DNCB, were shown to be highly sensitive to pretreatment with cyclophosphamide or X-irradiation, which suggests that these cells are B cells. The finding was confirmed by rosette-blocking experiments using an anti-immunoglobulin serum. It has already been shown that they are not directly related to antibody production. Possibly they represent precursors of antibody-producing cells. An increase in rosette formation, using non-antigenically related erythrocytes, was shown to parallel development of cell-mediated immunity (CMI) in the lymph nodes. These non-specifically induced RFC were also found to be B cells. Data were obtained which make it improbable that either proliferation or influx of B cells during development of CMI plays a major role in this increase. An alternative mechanism is suggested, while a possible role for these non-specifically induced RC is proposed."}
{"id": "PMID:1102452", "title": "Dosage adjustments of cefazolin according to the pharmacokinetics of this new cephalosporin.", "content": "Serum and urine levels of cefazolin were studied: 1. following a single 500mg intra-muscular dose of the drug in normal subjects (n = 10), in patients with renal impairment (n = 12) and in patients undergoing maintenance hemodialysis (n = 10); 2. following a single 500 mg intra-venous dose of the drug in normal subjects (n = 10). In normal subjects, serum half-lives averaged 1.75 hour after intravenous injection (Ke = 0.395) and 2.22 hours after intra-muscular injection (Ke = 0.312); urinary recovery of cefazolin over a six hours period amounted to 61% after intra-venous injection and to 47.5% after intra-muscular injection. The renal clearance of the drug approximated 40 ml/mn. A linear correlation (Ke = 0.022 + 0.0028 CrCl) was established between the overall elimination rate-constants and the creatinine clearances in the subjects under investigation. The various pharmacokinetic constants thus obtained can be used to calculate the maintenance doses, loading doses and dosage intervals adjusted according to creatinine clearances. These data actually yield dosage regimes adapted to each individual case according to the degree of renal function.", "contents": "Dosage adjustments of cefazolin according to the pharmacokinetics of this new cephalosporin. Serum and urine levels of cefazolin were studied: 1. following a single 500mg intra-muscular dose of the drug in normal subjects (n = 10), in patients with renal impairment (n = 12) and in patients undergoing maintenance hemodialysis (n = 10); 2. following a single 500 mg intra-venous dose of the drug in normal subjects (n = 10). In normal subjects, serum half-lives averaged 1.75 hour after intravenous injection (Ke = 0.395) and 2.22 hours after intra-muscular injection (Ke = 0.312); urinary recovery of cefazolin over a six hours period amounted to 61% after intra-venous injection and to 47.5% after intra-muscular injection. The renal clearance of the drug approximated 40 ml/mn. A linear correlation (Ke = 0.022 + 0.0028 CrCl) was established between the overall elimination rate-constants and the creatinine clearances in the subjects under investigation. The various pharmacokinetic constants thus obtained can be used to calculate the maintenance doses, loading doses and dosage intervals adjusted according to creatinine clearances. These data actually yield dosage regimes adapted to each individual case according to the degree of renal function."}
{"id": "PMID:1102453", "title": "[Antibody detection in infections with Serratia marcescens (author's transl)].", "content": "A report is given on the detection by agglutination and precipitation of antibodies against homologous strains in infections with Serratia marcescens. The results of the investigation indicate that antibody formation is likely if invasive forms are present. The agglutination titers were between 1:40 and 1:640. It can be presumed that, in addition to the patient's antibodies against the O antigen, antibodies are also formed against certain antigens with the character of ferments or toxins.", "contents": "[Antibody detection in infections with Serratia marcescens (author's transl)]. A report is given on the detection by agglutination and precipitation of antibodies against homologous strains in infections with Serratia marcescens. The results of the investigation indicate that antibody formation is likely if invasive forms are present. The agglutination titers were between 1:40 and 1:640. It can be presumed that, in addition to the patient's antibodies against the O antigen, antibodies are also formed against certain antigens with the character of ferments or toxins."}
{"id": "PMID:1102454", "title": "Properties of lymphocytes in chronic HBAg-positive hepatitis. An approach to pathogenetic problems.", "content": "Lymphocytes from 22 children affected by chronic hepatitis were studied and a comparison was made with a control group of 32 healthy children in the same age range with respect to PHA dose-response curves. E-rosette formation and membrane immunoglobulin (Ig). From the results it appeared that PHA dose-response curves were displaced to the left (low PHA dose), DNA synthesis was significantly higher for all PHA dosages tested, and that the plasma of chronic hepatitis patients acted neither as inhibitor nor stimulator. The percentage of E-rosette-forming cells decreased, but the total number was normal. The mean percentage of Ig-bearing cells was normal but the single values were spaced over a wide range. It can be assumed that, at least in the chronic stage, there is an enhanced response to PHA and an increased percentage of cells without markers either for T or for B cells.", "contents": "Properties of lymphocytes in chronic HBAg-positive hepatitis. An approach to pathogenetic problems. Lymphocytes from 22 children affected by chronic hepatitis were studied and a comparison was made with a control group of 32 healthy children in the same age range with respect to PHA dose-response curves. E-rosette formation and membrane immunoglobulin (Ig). From the results it appeared that PHA dose-response curves were displaced to the left (low PHA dose), DNA synthesis was significantly higher for all PHA dosages tested, and that the plasma of chronic hepatitis patients acted neither as inhibitor nor stimulator. The percentage of E-rosette-forming cells decreased, but the total number was normal. The mean percentage of Ig-bearing cells was normal but the single values were spaced over a wide range. It can be assumed that, at least in the chronic stage, there is an enhanced response to PHA and an increased percentage of cells without markers either for T or for B cells."}
{"id": "PMID:1102455", "title": "[Studies on the in vitro effects of cephalothin and gentamicin, alone and in combination, on proteus mirabilis and enterococci (author's transl)].", "content": "A combined antibiotic therapy is only useful in a few precisely defined clinical pictures where testing of the chemotherapeutics administered is required to determine their characteristics of action (antagonism, indifference, synergism) on the isolated organism. For the initial therapy of critical acute infections, simultaneous administration of cephalothin and gentamicin proved to be valuable. In the present study, the efficiency of these chemotherapeutics alone and in combination was investigated in a quantitative serial dilution test and with the membrane-filtration method. Thirty strains of Proteus mirabilis and enterococci showed only low sensitivity to the antibiotic alone. In combination, whereby gentamicin was at a constant level comparable to in vivo serum levels, an increase of bacteriostatic and bactericidal action could be demonstrated, especially for Proteus mirabilis. The antibacterial spectrum, the molecular-biological mode of action, clinical experience and possible side-effects of the cephalotin-gentamicin combination are discussed.", "contents": "[Studies on the in vitro effects of cephalothin and gentamicin, alone and in combination, on proteus mirabilis and enterococci (author's transl)]. A combined antibiotic therapy is only useful in a few precisely defined clinical pictures where testing of the chemotherapeutics administered is required to determine their characteristics of action (antagonism, indifference, synergism) on the isolated organism. For the initial therapy of critical acute infections, simultaneous administration of cephalothin and gentamicin proved to be valuable. In the present study, the efficiency of these chemotherapeutics alone and in combination was investigated in a quantitative serial dilution test and with the membrane-filtration method. Thirty strains of Proteus mirabilis and enterococci showed only low sensitivity to the antibiotic alone. In combination, whereby gentamicin was at a constant level comparable to in vivo serum levels, an increase of bacteriostatic and bactericidal action could be demonstrated, especially for Proteus mirabilis. The antibacterial spectrum, the molecular-biological mode of action, clinical experience and possible side-effects of the cephalotin-gentamicin combination are discussed."}
{"id": "PMID:1102457", "title": "The need for orthodontic treatment: a periodontist's point of view.", "content": "Data at hand suggests that occlusal traumatism is not causally related to gingival inflammation. Nor does occlusal traumatism affect the severity of existing inflammation. While an effect of occlusal traumatism on the spread of inflammation has been postulated, most observations seem to question such an interaction. Malposition of teeth has not been proven to be an etiologic factor in inflammatory periodontal disease nor could the degree of periodontal disease severity be consistently associated with improper tooth position. While malposed teeth may make plaque removal more difficult, specific oral hygiene measures should overcome this handicap. Orthodontic appliances may act as local gingival irritants, either directly or as plaque accumulants, and should be designed to minimize such irritations. Finally, orthodontic treatment, by properly aligning tilted or drifted teeth which also exhibit infraosseous lesions, may improve periodontal therapy at such sites.", "contents": "The need for orthodontic treatment: a periodontist's point of view. Data at hand suggests that occlusal traumatism is not causally related to gingival inflammation. Nor does occlusal traumatism affect the severity of existing inflammation. While an effect of occlusal traumatism on the spread of inflammation has been postulated, most observations seem to question such an interaction. Malposition of teeth has not been proven to be an etiologic factor in inflammatory periodontal disease nor could the degree of periodontal disease severity be consistently associated with improper tooth position. While malposed teeth may make plaque removal more difficult, specific oral hygiene measures should overcome this handicap. Orthodontic appliances may act as local gingival irritants, either directly or as plaque accumulants, and should be designed to minimize such irritations. Finally, orthodontic treatment, by properly aligning tilted or drifted teeth which also exhibit infraosseous lesions, may improve periodontal therapy at such sites."}
{"id": "PMID:1102458", "title": "A social perspective on need and demand for orthodontic treatment.", "content": "In addition to needs fostered by actual physical disabilities resulting from malocclusion, need and demand for orthodontic treatment may develop entirely apart from any consideration of health needs. In fact, improvement of physical function is not the primary motivation of many persons who receive orthodontic care. A variety of social factors are related to perceived needs for acceptable dentofacial appearance and to public demand for orthodontic services. Since early times human teeth have been the object of mutilations to satisfy sociocultural expectations. The mutilation, and even removal of teeth, to achieve dental cultural norms in, so-called, primitive societies may appear barbaric to an ethnocentric society. However, a significant sociocultural need can be met equally by the African or Brazilian who has had teeth filed to a point and by the Western adolescent who having had four teeth surgically removed to provide space in the arch then undergoes two years of orthodontic therapy to reposition and straighten the remaining teeth. From a sociological point of view, the need and desire of members of a society to achieve a culturally acceptable body image constitutes neither frivolity nor luxury. If children's teeth do not naturally meet the norm for esthetic dental appearance, the response of families to sociocultural expectations and pressures produces a culturally valid need for orthodontic intervention. Other social factors affecting perceived need and demand for orthodontic care are socioeconomic, desire for upward social mobility, and social change with regard to public attitudes toward the availability of dental health services and the increasing removal of financial barriers. Potential changes in present US orthodontic practice may be required if care is to be delivered to significantly larger segments of the population.", "contents": "A social perspective on need and demand for orthodontic treatment. In addition to needs fostered by actual physical disabilities resulting from malocclusion, need and demand for orthodontic treatment may develop entirely apart from any consideration of health needs. In fact, improvement of physical function is not the primary motivation of many persons who receive orthodontic care. A variety of social factors are related to perceived needs for acceptable dentofacial appearance and to public demand for orthodontic services. Since early times human teeth have been the object of mutilations to satisfy sociocultural expectations. The mutilation, and even removal of teeth, to achieve dental cultural norms in, so-called, primitive societies may appear barbaric to an ethnocentric society. However, a significant sociocultural need can be met equally by the African or Brazilian who has had teeth filed to a point and by the Western adolescent who having had four teeth surgically removed to provide space in the arch then undergoes two years of orthodontic therapy to reposition and straighten the remaining teeth. From a sociological point of view, the need and desire of members of a society to achieve a culturally acceptable body image constitutes neither frivolity nor luxury. If children's teeth do not naturally meet the norm for esthetic dental appearance, the response of families to sociocultural expectations and pressures produces a culturally valid need for orthodontic intervention. Other social factors affecting perceived need and demand for orthodontic care are socioeconomic, desire for upward social mobility, and social change with regard to public attitudes toward the availability of dental health services and the increasing removal of financial barriers. Potential changes in present US orthodontic practice may be required if care is to be delivered to significantly larger segments of the population."}
{"id": "PMID:1102459", "title": "Organization of the Navy Dental Corps.", "content": "The US Navy Dental Corps was established by an act of Congress in 1912. Its membership consists of dental officers of the Regular Navy and Naval Reserve. The primary mission of the Corps is to prevent or remedy dental conditions that may interfere with the performance of duty by members of the active naval forces. The organizational structure within which the Dental Corps functions is discussed at some length. The Chief of the Dental Corps, under the Surgeon General, is responsible for the planning and direction of programs providing dental care to Navy and Marine Corps personnel and others authorized by law. Until recently, the majority of dental personnel received only technical assistance control from the Bureau of Medicine and Surgery; but in consonance with the goals of improving management effectiveness and achieving efficient use of personnel and material resources, the Navy Dental Corps has undertaken a program of regionalization of its facilities. The establishment of regional dental centers under one command permits immediate response to the needs of populations and the activities they serve, while at the same time reducing layering of administrative and fiscal burdens of existing dental departments at various commands, which allows more dental officers to devote their time to dental health care delivery.", "contents": "Organization of the Navy Dental Corps. The US Navy Dental Corps was established by an act of Congress in 1912. Its membership consists of dental officers of the Regular Navy and Naval Reserve. The primary mission of the Corps is to prevent or remedy dental conditions that may interfere with the performance of duty by members of the active naval forces. The organizational structure within which the Dental Corps functions is discussed at some length. The Chief of the Dental Corps, under the Surgeon General, is responsible for the planning and direction of programs providing dental care to Navy and Marine Corps personnel and others authorized by law. Until recently, the majority of dental personnel received only technical assistance control from the Bureau of Medicine and Surgery; but in consonance with the goals of improving management effectiveness and achieving efficient use of personnel and material resources, the Navy Dental Corps has undertaken a program of regionalization of its facilities. The establishment of regional dental centers under one command permits immediate response to the needs of populations and the activities they serve, while at the same time reducing layering of administrative and fiscal burdens of existing dental departments at various commands, which allows more dental officers to devote their time to dental health care delivery."}
{"id": "PMID:1102460", "title": "Leukocyte migration inhibition in nickel dermatitis.", "content": "Leukocyte migration inhibitory factor assay was employed as an in vitro diagnostic aid in nickel dermatitis, the second most common contact dermatitis in North America. 15 patch test-positive and 5 patch test-negative patients, all giving a past history suggestive of nickel dermatitis, were investigated. Significant inhibition of leukocyte migration in both groups of patients was obtained only with nickel sulfate-albumin conjugate and not with unconjugated nickel sulfate. Specificity of this system was tested by utilizing an unrelated metallic albumin complex, and no inhibition was found. When patch testing is equivocal or contraindicated, this in vitro technique may be a practical alternative.", "contents": "Leukocyte migration inhibition in nickel dermatitis. Leukocyte migration inhibitory factor assay was employed as an in vitro diagnostic aid in nickel dermatitis, the second most common contact dermatitis in North America. 15 patch test-positive and 5 patch test-negative patients, all giving a past history suggestive of nickel dermatitis, were investigated. Significant inhibition of leukocyte migration in both groups of patients was obtained only with nickel sulfate-albumin conjugate and not with unconjugated nickel sulfate. Specificity of this system was tested by utilizing an unrelated metallic albumin complex, and no inhibition was found. When patch testing is equivocal or contraindicated, this in vitro technique may be a practical alternative."}
{"id": "PMID:1102461", "title": "Production of homocytotropic antibody against acid extracts isolated from group A streptococcal cell walls in intact and adrenalectomized rats.", "content": "Intact and adrenalectomized rats of male Sprague-Dawley were injected with acid-extracted materials isolated from group A streptococcal cell walls using aluminum gel as an adjuvant. We have clearly demonstrated the production of homocytotropic antibodies against this antigens in rats. However, the homocytotropic antibody titers in adrenalectomized rats were much higher than those in intact rats.", "contents": "Production of homocytotropic antibody against acid extracts isolated from group A streptococcal cell walls in intact and adrenalectomized rats. Intact and adrenalectomized rats of male Sprague-Dawley were injected with acid-extracted materials isolated from group A streptococcal cell walls using aluminum gel as an adjuvant. We have clearly demonstrated the production of homocytotropic antibodies against this antigens in rats. However, the homocytotropic antibody titers in adrenalectomized rats were much higher than those in intact rats."}
{"id": "PMID:1102462", "title": "Antiserum specific for reticulin of the bursa of fabricius.", "content": "Antiserum produced by immunizing rabbits with embryonic chicken spleen cells showed, after appropriate absorption with cell suspensions, specificity for fetal spleen cells in membrane immunofluorescence tests. However, when the original antiserum was absorbed with tissue homogenates and tested on cryostat tissue sections, it reacted specifically with the reticular framework which separates the cortical and medullary lymphocytes in the follicles of the adult bursa of Fabricius. It is suggested that the reticulin may influence bursal lymphoid differentiation.", "contents": "Antiserum specific for reticulin of the bursa of fabricius. Antiserum produced by immunizing rabbits with embryonic chicken spleen cells showed, after appropriate absorption with cell suspensions, specificity for fetal spleen cells in membrane immunofluorescence tests. However, when the original antiserum was absorbed with tissue homogenates and tested on cryostat tissue sections, it reacted specifically with the reticular framework which separates the cortical and medullary lymphocytes in the follicles of the adult bursa of Fabricius. It is suggested that the reticulin may influence bursal lymphoid differentiation."}
{"id": "PMID:1102463", "title": "Ontogenetic aspects of sexual dimorphism and the primary immune response to sheep erythrocytes in hamsters from prepuberty through senescence.", "content": "Ontogeny of the primary response to sheep erythrocytes of age-matched groups of male and female hamsters was studied at various chronological ages ranging from prepuberty to senescence. Selected organs were likewise weighed upon sacrifice to obtain developmental patterns. Adrenal weights were higher in the male, and pituitary weights were higher in the female; for both organs typical dimorphism was demonstrable by 36 days. Spleen weight and index favored the female by 46 days. Immunological sex dimorphism first appeared in groups injected at 53 days and autopsied at 58 days and persisted through senescence. Sexual dimorphism of antibody-mediated immunity, previously shown to favor the female in both the primary and secondary immune responses, thus follows the dimorphism of total amount of splenic lymphoid tissue and occurs shortly after realization of sexual maturity in the male. These findings support our previous suggestion of the suppressive effect of androgens on the antibody-mediated immune responsiveness of the male hamster.", "contents": "Ontogenetic aspects of sexual dimorphism and the primary immune response to sheep erythrocytes in hamsters from prepuberty through senescence. Ontogeny of the primary response to sheep erythrocytes of age-matched groups of male and female hamsters was studied at various chronological ages ranging from prepuberty to senescence. Selected organs were likewise weighed upon sacrifice to obtain developmental patterns. Adrenal weights were higher in the male, and pituitary weights were higher in the female; for both organs typical dimorphism was demonstrable by 36 days. Spleen weight and index favored the female by 46 days. Immunological sex dimorphism first appeared in groups injected at 53 days and autopsied at 58 days and persisted through senescence. Sexual dimorphism of antibody-mediated immunity, previously shown to favor the female in both the primary and secondary immune responses, thus follows the dimorphism of total amount of splenic lymphoid tissue and occurs shortly after realization of sexual maturity in the male. These findings support our previous suggestion of the suppressive effect of androgens on the antibody-mediated immune responsiveness of the male hamster."}
{"id": "PMID:1102464", "title": "Epoxides metabolically produced from some known carcinogens and from some clinically used drugs. I. Differences in mutagenicity.", "content": "The epoxide metabolites of two clinically used drugs and an experimental psychotropic agent, carbamazepine 10,11-oxide, cyproheptadine 10,11-oxide and cyclobenzaprine 10,11-oxide, were fully devoid of any mutagenic activity under conditions where K-region-epoxide metabolites of some known carcinogens, such as benzo(a)pyrene, proved to be potent frameshift mutational agents for Salmonella typhimurium TA 1537 and TA 1538. All epoxides tested were non-mutagenic for TA 1535, designed to detect substitution mutations. The 10,11-epoxides of the three drugs, carbamazepine, cyproheptadine and cyclobenzaprine, were not cytotoxic to any of the bacterial tester strains used, precluding that mutagenicity might have been overshadowed by cytotoxicity. When the mutagen, precursor, benzo(a)pyrene, was incubated together with TA 1537 and a mammalian microsomal preparation in the presence of a system generating the co-factor necessary for mono-oxygenase activity, activation to mutagenic species was observed which was dramatically increased in the presence of a potent epoxide hydratase inhibitor, 1,1,1-trichloropropene 2,3-oxide, suggesting epoxide(s) as the (or one of the) mutagenically active species metabolically produced in situ. None of these effects was observed with the three medical drugs. Moreover, the observation that the alkene oxide 4-phenylstyrene 7,8-oxide is mutagenic to the two strains TA 1537 and TA 1538 but the K-region arene oxide derived from 7,12-dimethylbenz(a)anthracene is inactive for the latter strain indicates that epoxidation of an aromatic double bond of a polycyclic hydrocarbon is neither a necessary nor a satisfying condition for frameshift mutagenesis to occur.", "contents": "Epoxides metabolically produced from some known carcinogens and from some clinically used drugs. I. Differences in mutagenicity. The epoxide metabolites of two clinically used drugs and an experimental psychotropic agent, carbamazepine 10,11-oxide, cyproheptadine 10,11-oxide and cyclobenzaprine 10,11-oxide, were fully devoid of any mutagenic activity under conditions where K-region-epoxide metabolites of some known carcinogens, such as benzo(a)pyrene, proved to be potent frameshift mutational agents for Salmonella typhimurium TA 1537 and TA 1538. All epoxides tested were non-mutagenic for TA 1535, designed to detect substitution mutations. The 10,11-epoxides of the three drugs, carbamazepine, cyproheptadine and cyclobenzaprine, were not cytotoxic to any of the bacterial tester strains used, precluding that mutagenicity might have been overshadowed by cytotoxicity. When the mutagen, precursor, benzo(a)pyrene, was incubated together with TA 1537 and a mammalian microsomal preparation in the presence of a system generating the co-factor necessary for mono-oxygenase activity, activation to mutagenic species was observed which was dramatically increased in the presence of a potent epoxide hydratase inhibitor, 1,1,1-trichloropropene 2,3-oxide, suggesting epoxide(s) as the (or one of the) mutagenically active species metabolically produced in situ. None of these effects was observed with the three medical drugs. Moreover, the observation that the alkene oxide 4-phenylstyrene 7,8-oxide is mutagenic to the two strains TA 1537 and TA 1538 but the K-region arene oxide derived from 7,12-dimethylbenz(a)anthracene is inactive for the latter strain indicates that epoxidation of an aromatic double bond of a polycyclic hydrocarbon is neither a necessary nor a satisfying condition for frameshift mutagenesis to occur."}
{"id": "PMID:1102466", "title": "The development of the nursing labor force in the United States: a basic analysis.", "content": "In this article, we draw together some basic material on the objectives character of the nursing labor force in the United States. First, we present a statistical and descriptive picture of the nursing labor force as it exists today. Then we analyze the historical development of that labor force up to about 1950, stressing its general integration into a hierarchical and concentrated health industry. Finally, we look at the development of the nursing labor force over the past 25 years in terms of factors such as sex, race, age educational credentials, labor force participation, place of work, and the hierarchical division of labor, discussing the general implications of the changes in these factors for worker organization in the health industry. Our main conclusion is that the nursing labor force is in a kiey position to lead a movement for the restructing of the health industry to serve the health needs of the American people rather than the profit and power requirements of the health employers. The primary, and difficult, task ahead is to build labor organization which will have the vision and unity to work this goal.", "contents": "The development of the nursing labor force in the United States: a basic analysis. In this article, we draw together some basic material on the objectives character of the nursing labor force in the United States. First, we present a statistical and descriptive picture of the nursing labor force as it exists today. Then we analyze the historical development of that labor force up to about 1950, stressing its general integration into a hierarchical and concentrated health industry. Finally, we look at the development of the nursing labor force over the past 25 years in terms of factors such as sex, race, age educational credentials, labor force participation, place of work, and the hierarchical division of labor, discussing the general implications of the changes in these factors for worker organization in the health industry. Our main conclusion is that the nursing labor force is in a kiey position to lead a movement for the restructing of the health industry to serve the health needs of the American people rather than the profit and power requirements of the health employers. The primary, and difficult, task ahead is to build labor organization which will have the vision and unity to work this goal."}
{"id": "PMID:1102467", "title": "The women's health movement.", "content": "This article provides a brief overview of the history, current status, and future directions of the Women's Health Movement. The links between feminism and women's health issues are drawn and reasons for their simultaneous occurrence are suggested. It is shown that health issues are seen by the Movement has suspended in the larger social context from which they arise.", "contents": "The women's health movement. This article provides a brief overview of the history, current status, and future directions of the Women's Health Movement. The links between feminism and women's health issues are drawn and reasons for their simultaneous occurrence are suggested. It is shown that health issues are seen by the Movement has suspended in the larger social context from which they arise."}
{"id": "PMID:1102468", "title": "The politics of birth control, 1920-1940: the impact of professionals.", "content": "Before the 1920s, a birth control movement arose in the United States out of socialist, feminist, and other radical groups concerned with women's rights and sexual freedom. After 1920 the birth control movement became gradually transformed into a respectable, nonradical reform cause, the recipient of large grants from big business, with women's rights secondary to an overriding concern with medical health and population control. This transformation was achieved through the professionalization of the birth control movement-that is, its takeover by professional experts, almost all male, in place of the radical amateur women, fighting for their own interests, who initiated it. The article examines two groups of professionals who were particularly influential in this transformation: doctors and academic eugenists. The former made birth control a medical issue, held back the development of popular sex education, and stifled a previously developing feminist approach to women's birth control needs. The later contributed racism to the birth control movement, helping to transform it into a population control movement with racist and anti-feminist overtones. Both groups, while they made contributions to the technology of contraception, simultaneously held back the spread of birth control by transforming the campaign for it from a popular, participatory cause to a professional staff lobbying operation.", "contents": "The politics of birth control, 1920-1940: the impact of professionals. Before the 1920s, a birth control movement arose in the United States out of socialist, feminist, and other radical groups concerned with women's rights and sexual freedom. After 1920 the birth control movement became gradually transformed into a respectable, nonradical reform cause, the recipient of large grants from big business, with women's rights secondary to an overriding concern with medical health and population control. This transformation was achieved through the professionalization of the birth control movement-that is, its takeover by professional experts, almost all male, in place of the radical amateur women, fighting for their own interests, who initiated it. The article examines two groups of professionals who were particularly influential in this transformation: doctors and academic eugenists. The former made birth control a medical issue, held back the development of popular sex education, and stifled a previously developing feminist approach to women's birth control needs. The later contributed racism to the birth control movement, helping to transform it into a population control movement with racist and anti-feminist overtones. Both groups, while they made contributions to the technology of contraception, simultaneously held back the spread of birth control by transforming the campaign for it from a popular, participatory cause to a professional staff lobbying operation."}
{"id": "PMID:1102469", "title": "Sexual surgery in late-nineteenth-century America.", "content": "This article describes how the tendency of post-Civil War medicine accorded with general social tendencies. Doctors' attitudes toward women reflected their anxiety about female emancipation and changing sex roles. The specialty of gynecology emerged in significant part as a reaction to female emancipation. The strong surgical emphasis in American gynecology practice has continued. The operations, clitoridectomy and female castration, were intended to reimpose the traditional sexual order (the term \"castration\" and \"female circumcision\" implied women had become men). The paper goes on to describe the beginnings of psychosomatic medicine, the circularity of gynecologists' treatment of women, and the effects of this treatment. The operations were in part the expression of a symbiotic relation between doctor and patient. Because they expressed widespread and deep-seated beliefs, they were difficult to challenge.", "contents": "Sexual surgery in late-nineteenth-century America. This article describes how the tendency of post-Civil War medicine accorded with general social tendencies. Doctors' attitudes toward women reflected their anxiety about female emancipation and changing sex roles. The specialty of gynecology emerged in significant part as a reaction to female emancipation. The strong surgical emphasis in American gynecology practice has continued. The operations, clitoridectomy and female castration, were intended to reimpose the traditional sexual order (the term \"castration\" and \"female circumcision\" implied women had become men). The paper goes on to describe the beginnings of psychosomatic medicine, the circularity of gynecologists' treatment of women, and the effects of this treatment. The operations were in part the expression of a symbiotic relation between doctor and patient. Because they expressed widespread and deep-seated beliefs, they were difficult to challenge."}
{"id": "PMID:1102472", "title": "Denaturation of thermophilic and mesophilic 6-phosphogluconate dehydrogenase by 8M urea.", "content": "The denaturation reaction of 6-phosphogluconate dehydrogenase (6-phospho-D-gluconate: NADP oxidoreductase (decarboxylating EC 1.1.1.44) from B. Stearothermophilus and E. coli in 8 M urea has been compared. The rates of denaturation were evaluated from the fluorescence quenching that occurs at 334 nm. The thermophilic enzyme has been found about ten times more resistant to the action of the denaturating agent, and showed a biphasic denaturation process, whereas the E. coli enzyme followed a single first-order kinetics.", "contents": "Denaturation of thermophilic and mesophilic 6-phosphogluconate dehydrogenase by 8M urea. The denaturation reaction of 6-phosphogluconate dehydrogenase (6-phospho-D-gluconate: NADP oxidoreductase (decarboxylating EC 1.1.1.44) from B. Stearothermophilus and E. coli in 8 M urea has been compared. The rates of denaturation were evaluated from the fluorescence quenching that occurs at 334 nm. The thermophilic enzyme has been found about ten times more resistant to the action of the denaturating agent, and showed a biphasic denaturation process, whereas the E. coli enzyme followed a single first-order kinetics."}
{"id": "PMID:1102475", "title": "Treatment of prostatic cancer with Estracyt (estramustine phosphate).", "content": "The experience gained with Estracyt, kindly supplied by AB LEO, Sweden, is reported. On the basis of former data in the literature we only used the drug in estrogen resistant and advanced cases. Estracyt (estramustine phosphate) is a nitrogen mustard derivative of the urethan type, attached to oestradiol-17-phosphate. In histologically verified cases, it was administered in daily doses of 300 mg intravenously for three weeks, followed by maintenance doses of 300 mg a week in tablets for three months. During treatment, liver and bone marrow function was checked systematically. The changes in morphological picture were studied by means of biopsies during and at the end of treatment. In agreement with the data in the literature a favourable effect was observed in estrogen resistant patients, with no toxic effect whatever on the bone marrow. At the same time GOT and GPT and BSP retention examinations demonstrated a hepatotoxic side effect. The pathological values returned to normal after withdrawal of the drug. Histological examinations showed that the tumour cells had changed but failed to disappear after treatment.", "contents": "Treatment of prostatic cancer with Estracyt (estramustine phosphate). The experience gained with Estracyt, kindly supplied by AB LEO, Sweden, is reported. On the basis of former data in the literature we only used the drug in estrogen resistant and advanced cases. Estracyt (estramustine phosphate) is a nitrogen mustard derivative of the urethan type, attached to oestradiol-17-phosphate. In histologically verified cases, it was administered in daily doses of 300 mg intravenously for three weeks, followed by maintenance doses of 300 mg a week in tablets for three months. During treatment, liver and bone marrow function was checked systematically. The changes in morphological picture were studied by means of biopsies during and at the end of treatment. In agreement with the data in the literature a favourable effect was observed in estrogen resistant patients, with no toxic effect whatever on the bone marrow. At the same time GOT and GPT and BSP retention examinations demonstrated a hepatotoxic side effect. The pathological values returned to normal after withdrawal of the drug. Histological examinations showed that the tumour cells had changed but failed to disappear after treatment."}
{"id": "PMID:1102484", "title": "Ara-A and IDU therapy of human superficial herpetic keratitis.", "content": "Ara-A ointment was compared to IDU ointment in patients with dendritic herpes simplex virus infection of the corneal epithelium. Twenty-eight patients were treated with Ara-A ointment and twenty-four with IDU ointment. The lesions healed in 5.1 days with Ara-A and in 6.9 days with IDU. This drug was given in a double-controlled manner, so that neither the patient, nor the investigator knew which drug the patient was receiving. The patient groups were comparable as to length of the dendritic lesion and duration of symptoms. The adverse reactions to each of these drugs were comparable and in no case was there any permanent ocular change from drug use.", "contents": "Ara-A and IDU therapy of human superficial herpetic keratitis. Ara-A ointment was compared to IDU ointment in patients with dendritic herpes simplex virus infection of the corneal epithelium. Twenty-eight patients were treated with Ara-A ointment and twenty-four with IDU ointment. The lesions healed in 5.1 days with Ara-A and in 6.9 days with IDU. This drug was given in a double-controlled manner, so that neither the patient, nor the investigator knew which drug the patient was receiving. The patient groups were comparable as to length of the dendritic lesion and duration of symptoms. The adverse reactions to each of these drugs were comparable and in no case was there any permanent ocular change from drug use."}
{"id": "PMID:1102485", "title": "Use of a platelet-fibrinogen-thrombin mixture as a corneal adhesive: experiments with sutureless lamellar keratoplasty in the rabbit.", "content": "A platelet-fibrinogen-thrombin mixture utilizing autologous platelets was studied for its potential as a corneal adhesive. In the rabbit it demonstrated sufficient adhesive properties to allow 50 per cent of lamellar keratoplasties (autotransplants) to remain in place without the use of sutures. The mixture retains significant adhesive properties for four to six days. It is simple to prepare and apply. It also appears nonantigenic and nontoxic to the cornea; it does not incite inflammation, nor interfere with corneal clarity or the regrowth of corneal epithelium.", "contents": "Use of a platelet-fibrinogen-thrombin mixture as a corneal adhesive: experiments with sutureless lamellar keratoplasty in the rabbit. A platelet-fibrinogen-thrombin mixture utilizing autologous platelets was studied for its potential as a corneal adhesive. In the rabbit it demonstrated sufficient adhesive properties to allow 50 per cent of lamellar keratoplasties (autotransplants) to remain in place without the use of sutures. The mixture retains significant adhesive properties for four to six days. It is simple to prepare and apply. It also appears nonantigenic and nontoxic to the cornea; it does not incite inflammation, nor interfere with corneal clarity or the regrowth of corneal epithelium."}
{"id": "PMID:1102488", "title": "Studies on the effectiveness of liver and bacterial beta-glucuronidase enzymes in the hydrolysis of urinary beta-glucosiduronides of some tryptophan metabolites.", "content": "The effectiveness of Escherichia coli and bovine liver beta-glucuronidases in the hydrolysis of the urinary beta-glucosiduronides of tryptophan metabolites was studied. Moreover, the effect of the prolonged contact of these conjugates to the urinary enzyme was investigated in the first and second voiding urine samples. It is found that both enzymes have no important role in releasing the free carcinogens from their glucosiduronides. The presence of free carcinogens could be attributed to the spontaneous hydrolysis of some labile conjugates. However, the prolonged contact of the freely active substances during the sleeping hours with the epithelium of the bladder may enhance the process of bladder carcinogenicity. The increased accumulation of these metabolites in the first voiding urine could be interpreted in terms of their rate of excretion rather than by the enzymatic hydrolysis of their conjugates.", "contents": "Studies on the effectiveness of liver and bacterial beta-glucuronidase enzymes in the hydrolysis of urinary beta-glucosiduronides of some tryptophan metabolites. The effectiveness of Escherichia coli and bovine liver beta-glucuronidases in the hydrolysis of the urinary beta-glucosiduronides of tryptophan metabolites was studied. Moreover, the effect of the prolonged contact of these conjugates to the urinary enzyme was investigated in the first and second voiding urine samples. It is found that both enzymes have no important role in releasing the free carcinogens from their glucosiduronides. The presence of free carcinogens could be attributed to the spontaneous hydrolysis of some labile conjugates. However, the prolonged contact of the freely active substances during the sleeping hours with the epithelium of the bladder may enhance the process of bladder carcinogenicity. The increased accumulation of these metabolites in the first voiding urine could be interpreted in terms of their rate of excretion rather than by the enzymatic hydrolysis of their conjugates."}
{"id": "PMID:1102491", "title": "Effect of microbial substances of different origins on the growth of Mycobacterium leprae.", "content": "Mycobacterium leprae multiplied in media enriched with substances originating from other mycobacteria, from non-acid-fast Actinomycetales or from gram-positive or gram-negative Eubacteriales. Most of the M. leprae strains did not grow on a synthetic medium containing the amino acids present in M. smegmatis, but the growth-promoting effect of sonic extracts of this organism indicated that substances of bacterial origin, other than amino acids, do act as growth factors. The identification of the growing, acid-fast microorganisms with M. leprae was verified by their ability to oxidize D-3,4-dihydroxyphenylalanine (D-dopa test). M. leprae did not multiply on Nakamura medium unless at least 40% of medium NM7 enriched by bacterial substances was present. Adequate aeration was essential for multiplication of M. leprae in enriched NM7 and No. 3 media.", "contents": "Effect of microbial substances of different origins on the growth of Mycobacterium leprae. Mycobacterium leprae multiplied in media enriched with substances originating from other mycobacteria, from non-acid-fast Actinomycetales or from gram-positive or gram-negative Eubacteriales. Most of the M. leprae strains did not grow on a synthetic medium containing the amino acids present in M. smegmatis, but the growth-promoting effect of sonic extracts of this organism indicated that substances of bacterial origin, other than amino acids, do act as growth factors. The identification of the growing, acid-fast microorganisms with M. leprae was verified by their ability to oxidize D-3,4-dihydroxyphenylalanine (D-dopa test). M. leprae did not multiply on Nakamura medium unless at least 40% of medium NM7 enriched by bacterial substances was present. Adequate aeration was essential for multiplication of M. leprae in enriched NM7 and No. 3 media."}
{"id": "PMID:1102487", "title": "Diuresis and improved renal hemodynamics produced by prostaglandin E1 in the dog with norepinephrine-induced acute renal failure.", "content": "Unilateral acute renal failure was induced in dogs by renal artery infusion of norepinephrine. Renal hemodynamics and function were studied before and 5 days after norepinephrine infusion, and during subsequent renal artery infusion of prostaglandin E1. After norepinephrine, the infused kidney exhibited decreased urine volume, sodium excretion, and creatinine clearance. Cortical blood flow, determined with radioactive microspheres, also decreased. No redistribution of intracortical blood flow occurred. Arteriographically, proximal vessels were constricted and the cortical nephrogram density diminished. During prostaglandin E1 infusion, urine volume, sodium excretion, and cortical blood flow increased. Creatinine clearance did not change significantly. Blood flow was redistributed from the outer third to the inner third of the cortex. Proximal vessels were dilated and the cortical nephrogram density was enhanced. The apparent dissociation of cortical blood flow from creatinine clearance suggests that prostaglandin E1-induced diuresis and natriuresis result from a mechanism other than increased glomerular filtration.", "contents": "Diuresis and improved renal hemodynamics produced by prostaglandin E1 in the dog with norepinephrine-induced acute renal failure. Unilateral acute renal failure was induced in dogs by renal artery infusion of norepinephrine. Renal hemodynamics and function were studied before and 5 days after norepinephrine infusion, and during subsequent renal artery infusion of prostaglandin E1. After norepinephrine, the infused kidney exhibited decreased urine volume, sodium excretion, and creatinine clearance. Cortical blood flow, determined with radioactive microspheres, also decreased. No redistribution of intracortical blood flow occurred. Arteriographically, proximal vessels were constricted and the cortical nephrogram density diminished. During prostaglandin E1 infusion, urine volume, sodium excretion, and cortical blood flow increased. Creatinine clearance did not change significantly. Blood flow was redistributed from the outer third to the inner third of the cortex. Proximal vessels were dilated and the cortical nephrogram density was enhanced. The apparent dissociation of cortical blood flow from creatinine clearance suggests that prostaglandin E1-induced diuresis and natriuresis result from a mechanism other than increased glomerular filtration."}
{"id": "PMID:1102496", "title": "LH and FSH response to synthetic LH-RH in children and adolescents with Turner's and Klinefelter's syndrome.", "content": "Stimulation with LH-RH (luteinizing hormone-releasing hormone) was performed in 29 children and adolescents with Turner's syndrome (15 less than 13 years old), and in 11 patients with Klinefelter's syndrome (7 before puberty). Synthetic LH-RH was injected intravenously in a dose of 25 mug/m2; plasma LH and FSH were determined radioimmunologically. The results show that patients with Turner's syndrome had pathologically elevated LH and FSH values at all ages. Normal LH and FSH results before and after LH-RH were found in 3 patients with X0/XX karyotype who had spontaneous menstruation. The 2 adult patients with Klinefelter's syndrome showed an exaggerated LH response and excessively high FSH levels before and after LH-RH, consistent with hypergonadotropic hypogonadism. Six out of 7 prepubertal boys with Klinefelter's syndrome, had a normal LH and FSH response to LH-RH. Only 1 prepubertal boy with cryptorchidism and genital maliformation in addition to the Klinefelter's syndrome had pathological values. The normal gonadotropin results in prepubertal patients with Klinefelter's syndrome show that their testes apparently loose the negative feedback activity on gonadotropin secretion only during puberty, at a time when tubular hyalinization appears. This is in contrast to Turner patients with gonadal dysgenesis who have hypergondadotropic hypogonadism already early in childhood.", "contents": "LH and FSH response to synthetic LH-RH in children and adolescents with Turner's and Klinefelter's syndrome. Stimulation with LH-RH (luteinizing hormone-releasing hormone) was performed in 29 children and adolescents with Turner's syndrome (15 less than 13 years old), and in 11 patients with Klinefelter's syndrome (7 before puberty). Synthetic LH-RH was injected intravenously in a dose of 25 mug/m2; plasma LH and FSH were determined radioimmunologically. The results show that patients with Turner's syndrome had pathologically elevated LH and FSH values at all ages. Normal LH and FSH results before and after LH-RH were found in 3 patients with X0/XX karyotype who had spontaneous menstruation. The 2 adult patients with Klinefelter's syndrome showed an exaggerated LH response and excessively high FSH levels before and after LH-RH, consistent with hypergonadotropic hypogonadism. Six out of 7 prepubertal boys with Klinefelter's syndrome, had a normal LH and FSH response to LH-RH. Only 1 prepubertal boy with cryptorchidism and genital maliformation in addition to the Klinefelter's syndrome had pathological values. The normal gonadotropin results in prepubertal patients with Klinefelter's syndrome show that their testes apparently loose the negative feedback activity on gonadotropin secretion only during puberty, at a time when tubular hyalinization appears. This is in contrast to Turner patients with gonadal dysgenesis who have hypergondadotropic hypogonadism already early in childhood."}
{"id": "PMID:1102497", "title": "Plasma gonadotropin (LH and FSH) concentrations in repubertal and pubertal children upon stimulation by LH-releasing hormone.", "content": "The simultaneous determination of LH and FSH were carried out in a single plasma sample by double antibody radioimmunoassays in a total of 26 prepubertal and pubertal children. The blood samples were taken before and after i.v. application of 25 mug synthetic LH-RH. Plasma LH and FSH concentrations found in prepubertal boys and girls at beginning of the test and at the time of maximum response were compared with those measured in pubertal boys and girls. The mean basal levels of LH and FSH are in group I (8 prepubertal girls): LH 0.6+/-0.7 (SD) mIU/ml, FSH 1.5+/-0.8 mIU/ml; in group II (6 prepubertal boys): LH 1.8+/-1.0 mIU/ml, FSH 2.0+/-0.8 mIU/ml; in group III (6 pubertal girls): LH 4.1+/-2.0 mIU/ml, FSH 4.7+/-2.8 mIU/ml; in group IV (6 pubertal boys): LH 3.5+/-2.3 mIU/ml, FSH2.0+/-1.2 mIU/ml. The mean maximum response of LH and FSH after stimulation are in group I: LH 4.1+/-2.3 mIU/ml, FSH 11.7+/-3.7 mIU/ml; in group II: LH 4.2+/-1.0 mIU/ml, FSH 6.0+/-1.1 mIU/ml; in group III: LH 19.7+/-10.6 mIU/ml, FSH 12.8+/-5.7 mIU/ml; in group IV: LH 13.4+/-5.7 mIU/ml, FSH 3.8+/-0.9 mIU/ml. It is suggested that without stimulation of the pituitary with LH-RH, plasma LH and FSH levels are not reliable parameters to test the pituitary gonadotropin reserve.", "contents": "Plasma gonadotropin (LH and FSH) concentrations in repubertal and pubertal children upon stimulation by LH-releasing hormone. The simultaneous determination of LH and FSH were carried out in a single plasma sample by double antibody radioimmunoassays in a total of 26 prepubertal and pubertal children. The blood samples were taken before and after i.v. application of 25 mug synthetic LH-RH. Plasma LH and FSH concentrations found in prepubertal boys and girls at beginning of the test and at the time of maximum response were compared with those measured in pubertal boys and girls. The mean basal levels of LH and FSH are in group I (8 prepubertal girls): LH 0.6+/-0.7 (SD) mIU/ml, FSH 1.5+/-0.8 mIU/ml; in group II (6 prepubertal boys): LH 1.8+/-1.0 mIU/ml, FSH 2.0+/-0.8 mIU/ml; in group III (6 pubertal girls): LH 4.1+/-2.0 mIU/ml, FSH 4.7+/-2.8 mIU/ml; in group IV (6 pubertal boys): LH 3.5+/-2.3 mIU/ml, FSH2.0+/-1.2 mIU/ml. The mean maximum response of LH and FSH after stimulation are in group I: LH 4.1+/-2.3 mIU/ml, FSH 11.7+/-3.7 mIU/ml; in group II: LH 4.2+/-1.0 mIU/ml, FSH 6.0+/-1.1 mIU/ml; in group III: LH 19.7+/-10.6 mIU/ml, FSH 12.8+/-5.7 mIU/ml; in group IV: LH 13.4+/-5.7 mIU/ml, FSH 3.8+/-0.9 mIU/ml. It is suggested that without stimulation of the pituitary with LH-RH, plasma LH and FSH levels are not reliable parameters to test the pituitary gonadotropin reserve."}
{"id": "PMID:1102499", "title": "Immunofluorescence demonstration of avidin in the immature chick oviduct epithelium after progesterone.", "content": "Immature chicks were used for the experiments 1-2 days after hatching. A group of chicks were injected with 5 mg of progesterone and sacrificed 1 or 24 hr later. An other group of chicks were injected daily for 9 days with 5 mg of diethylstilbestrol and thereafter with single injection of progesterone. Cryostat sections were incubated with rabbit anti-avidin serum and with fluorescein labelled antirabbit globulin for fluorescence histochemistry. In the control animals no epithelial cells of the oviduct were fluorescence positive independently whether or not the animals were pretreated with diethylstilbestrol. One hour after administration of progesterone epithelial cells showed occasionally a slight synthesis of avidin. 24 hours after the injection of progesterone most, however never all, of the epithelial cells showed avidin in the apical part of the cell. The fluorescence reaction was clearly more intense if the animals were estrogen-primed. Diethylstilbestrol caused a differentiation of oviductal glands which were, however, only occasionally avidin positive after progesterone. These results suggest that primitive oviductal cells can produce avidin without preceding differentiation whereas estrogen causes a differentiation of new line of cells which have regularly lost their capacity of avidin synthesis after progesterone administration.", "contents": "Immunofluorescence demonstration of avidin in the immature chick oviduct epithelium after progesterone. Immature chicks were used for the experiments 1-2 days after hatching. A group of chicks were injected with 5 mg of progesterone and sacrificed 1 or 24 hr later. An other group of chicks were injected daily for 9 days with 5 mg of diethylstilbestrol and thereafter with single injection of progesterone. Cryostat sections were incubated with rabbit anti-avidin serum and with fluorescein labelled antirabbit globulin for fluorescence histochemistry. In the control animals no epithelial cells of the oviduct were fluorescence positive independently whether or not the animals were pretreated with diethylstilbestrol. One hour after administration of progesterone epithelial cells showed occasionally a slight synthesis of avidin. 24 hours after the injection of progesterone most, however never all, of the epithelial cells showed avidin in the apical part of the cell. The fluorescence reaction was clearly more intense if the animals were estrogen-primed. Diethylstilbestrol caused a differentiation of oviductal glands which were, however, only occasionally avidin positive after progesterone. These results suggest that primitive oviductal cells can produce avidin without preceding differentiation whereas estrogen causes a differentiation of new line of cells which have regularly lost their capacity of avidin synthesis after progesterone administration."}
{"id": "PMID:1102500", "title": "Fluorescamine as a histochemical reagent: demonstration of polypeptide hormone-secreting cells.", "content": "Fluorescamine is a useful fluorescence microscopic reagent for the demonstration of certain peptide hormone-secreting cells in formaldehyde-fixed tissues. Among the cells demonstrated are the pituitary GH cells, the gastrin cells, the insulin cells and the thyroid C cells. In the latter cell system degranulation brings about a marked decrease in the intensity of the fluorescamine-induced fluorescence suggesting that the fluorogenic component is associated with the secretory granules. In models neither of the hormones tested--GH, gastrin, insulin--gave fluorescamine-induced fluorescence after pretreatment with formaldehyde. It is suggested that the fluorescamine-induced fluorescence of the endocrine cells demonstrates granular components distinct from the hormones.", "contents": "Fluorescamine as a histochemical reagent: demonstration of polypeptide hormone-secreting cells. Fluorescamine is a useful fluorescence microscopic reagent for the demonstration of certain peptide hormone-secreting cells in formaldehyde-fixed tissues. Among the cells demonstrated are the pituitary GH cells, the gastrin cells, the insulin cells and the thyroid C cells. In the latter cell system degranulation brings about a marked decrease in the intensity of the fluorescamine-induced fluorescence suggesting that the fluorogenic component is associated with the secretory granules. In models neither of the hormones tested--GH, gastrin, insulin--gave fluorescamine-induced fluorescence after pretreatment with formaldehyde. It is suggested that the fluorescamine-induced fluorescence of the endocrine cells demonstrates granular components distinct from the hormones."}
{"id": "PMID:1102501", "title": "Distribution and properties of glucagon immunoreactivity in the digestive tract of various mammals: an immunohistochemical and immunochemical study.", "content": "Numerous epithelial cells in the oxyntic gland area of the stomach of cat and dog appear to store pancreatic-type glucagon, as evidenced by immunohistochemistry and--in the case of the dog--also by radioimmunoanalysis. No glucagon immunoreactive cells are found in the antropyloric mucosa. Disseminated cells in the intestinal mucosa store gut-type glucagon. In all species studied, these cells predominate in ileum and colon.", "contents": "Distribution and properties of glucagon immunoreactivity in the digestive tract of various mammals: an immunohistochemical and immunochemical study. Numerous epithelial cells in the oxyntic gland area of the stomach of cat and dog appear to store pancreatic-type glucagon, as evidenced by immunohistochemistry and--in the case of the dog--also by radioimmunoanalysis. No glucagon immunoreactive cells are found in the antropyloric mucosa. Disseminated cells in the intestinal mucosa store gut-type glucagon. In all species studied, these cells predominate in ileum and colon."}
{"id": "PMID:1102502", "title": "Canine salmonellosis: prevalence, epizootiology, signs, and public health significance.", "content": "Of 53 salmonella serotypes isolated from dogs, S typhimurium and S anatum have been the most commonly isolated. Surveys have disclosed that over 20% of the dogs in a population may be infected. Simultaneous, multiple infections with 2 or more serotypes are not unusual. Nonclinical salmonellosis occurs in most cases. The severe form of the disease is manifested by diarrhea, vomiting, fever, depression, abortion, and death. Dogs may remain carriers and fecal shedders and thus serve as sources of salmonellosis for man and other animals. A number of documented transmissions from dogs to human beings have been recorded. Such infections in man have been severe.", "contents": "Canine salmonellosis: prevalence, epizootiology, signs, and public health significance. Of 53 salmonella serotypes isolated from dogs, S typhimurium and S anatum have been the most commonly isolated. Surveys have disclosed that over 20% of the dogs in a population may be infected. Simultaneous, multiple infections with 2 or more serotypes are not unusual. Nonclinical salmonellosis occurs in most cases. The severe form of the disease is manifested by diarrhea, vomiting, fever, depression, abortion, and death. Dogs may remain carriers and fecal shedders and thus serve as sources of salmonellosis for man and other animals. A number of documented transmissions from dogs to human beings have been recorded. Such infections in man have been severe."}
{"id": "PMID:1102503", "title": "The cellular changes during osteogenesis in bone and bone marrow composite autografts.", "content": "Osteogenesis in and around autografts has been studied in the hope of identifying the osteoblast precursor. Fresh autografts of cortical bone with its constituent bone marrow were implanted into intramuscular sites in rats. The grafts were removed at intervals over a four week period and examined by light and electron microscopy. A chronological sequence of degenerative, reparative and differentiative events was found and described. The relative contributions of the host and graft components to post-grafting osteogenesis are discussed and the problem of the origin of the osteoblast cells is examined, taking into account present views about the interrelationships between skeletal connective tissue cells and their precursors. It is concluded that, in the investigation described, the presence of host granulation tissue was necessary for osteogenesis and it is postulated that osteoblast progenitor cells can arise from this granulation tissue, their differentiation along the osteogenic line being stimulated in some way by the presence of the graft bone.", "contents": "The cellular changes during osteogenesis in bone and bone marrow composite autografts. Osteogenesis in and around autografts has been studied in the hope of identifying the osteoblast precursor. Fresh autografts of cortical bone with its constituent bone marrow were implanted into intramuscular sites in rats. The grafts were removed at intervals over a four week period and examined by light and electron microscopy. A chronological sequence of degenerative, reparative and differentiative events was found and described. The relative contributions of the host and graft components to post-grafting osteogenesis are discussed and the problem of the origin of the osteoblast cells is examined, taking into account present views about the interrelationships between skeletal connective tissue cells and their precursors. It is concluded that, in the investigation described, the presence of host granulation tissue was necessary for osteogenesis and it is postulated that osteoblast progenitor cells can arise from this granulation tissue, their differentiation along the osteogenic line being stimulated in some way by the presence of the graft bone."}
{"id": "PMID:1102504", "title": "The ultrastructure of the anterior pituitary gland of the vole, Microtus agrestis, in normal and experimentally manipulated animals.", "content": "The ultrastructural appearance of the various types of cell present in the anterior pituitary of the vole has been described. There was a great measure of similarity between the cytological picture in this species and in the rat. Prolactotrophs contained the largest secretory granules, which were of variable shape; the granules of somatotrophs, whilst only slightly smaller than those of prolactotrophs, were invariably round, and of more uniform size; corticotrophs were represented by cells which were extremely angular, and whose secretory granules, besides being smaller than those of somatotrophs, were arrayed around the periphery of the cell below the plasma membrane; gonadotrophs contained granules of a similar size to those found in cortiocotrophs, but were found throughout the cytoplasm of the cells, whic were round to ovoid in shape; thyrotrophs contained the smallest granules of all, the shape of the cell itself bein angular...", "contents": "The ultrastructure of the anterior pituitary gland of the vole, Microtus agrestis, in normal and experimentally manipulated animals. The ultrastructural appearance of the various types of cell present in the anterior pituitary of the vole has been described. There was a great measure of similarity between the cytological picture in this species and in the rat. Prolactotrophs contained the largest secretory granules, which were of variable shape; the granules of somatotrophs, whilst only slightly smaller than those of prolactotrophs, were invariably round, and of more uniform size; corticotrophs were represented by cells which were extremely angular, and whose secretory granules, besides being smaller than those of somatotrophs, were arrayed around the periphery of the cell below the plasma membrane; gonadotrophs contained granules of a similar size to those found in cortiocotrophs, but were found throughout the cytoplasm of the cells, whic were round to ovoid in shape; thyrotrophs contained the smallest granules of all, the shape of the cell itself bein angular..."}
{"id": "PMID:1102505", "title": "The fate of heterotopic isografts and allografts of mouse yolk sac epithelium.", "content": "As a contribution to the problem of the immunological privilege of the fetus it was decided to investigate the fate of yolk sac grafts in mice. Ten-day post-coitum mouse visceral yolk sac was transplanted to a dermal tunnel in the flank of isogeneic animals. In this site the yolk sac was capable of surviving for at least 15 days past the normal duration of pregnancy. Yolk sac was transplanted to a similar site in allogeneic animals and was found to be destroyed by an immunological reaction within 13 days. Orthotopic skin grafts between the same strains of mice used in the yolk sac transplantation were rejected in first-set fashion within 13 days. A limited series of host animals, previously sensitized by visceral yolk sac, rejected orthotopic skin grafts in second-set fashion. These findings indicate that the cells of the visceral yolk sac in the mouse contain transplantation antigens. Possible reasons why such antigens do not lead to fetal rejection during pregnancy are considered.", "contents": "The fate of heterotopic isografts and allografts of mouse yolk sac epithelium. As a contribution to the problem of the immunological privilege of the fetus it was decided to investigate the fate of yolk sac grafts in mice. Ten-day post-coitum mouse visceral yolk sac was transplanted to a dermal tunnel in the flank of isogeneic animals. In this site the yolk sac was capable of surviving for at least 15 days past the normal duration of pregnancy. Yolk sac was transplanted to a similar site in allogeneic animals and was found to be destroyed by an immunological reaction within 13 days. Orthotopic skin grafts between the same strains of mice used in the yolk sac transplantation were rejected in first-set fashion within 13 days. A limited series of host animals, previously sensitized by visceral yolk sac, rejected orthotopic skin grafts in second-set fashion. These findings indicate that the cells of the visceral yolk sac in the mouse contain transplantation antigens. Possible reasons why such antigens do not lead to fetal rejection during pregnancy are considered."}
{"id": "PMID:1102508", "title": "Rapamycin (AY-22,989), a new antifungal antibiotic. I. Taxonomy of the producing streptomycete and isolation of the active principle.", "content": "A streptomycete was isolated from an Easter Island soil sample and found to inhibit Candida albicans, Microsporum gypseum and Trichophyton granulosum. The antibiotic-producing microorganism was characterized and identified as Streptomyces hygroscopicus. The antifungal principle was extracted with organic solvent from the mycelium, isolated in crystalline form and named rapamycin. Rapamycin is mainly active against Candida albicans; minimum inhibitory concentration against ten strains ranged from 0.02 to 0.2 mug/ml. Its apparent activity against Microsporum gypseum and Trichophyton granulosum is lower because of its instability in culture media on prolonged incubation required by these fungi. No activity was observed against gram-positive and gram-negative bacteria. Acute toxicity in mice is low.", "contents": "Rapamycin (AY-22,989), a new antifungal antibiotic. I. Taxonomy of the producing streptomycete and isolation of the active principle. A streptomycete was isolated from an Easter Island soil sample and found to inhibit Candida albicans, Microsporum gypseum and Trichophyton granulosum. The antibiotic-producing microorganism was characterized and identified as Streptomyces hygroscopicus. The antifungal principle was extracted with organic solvent from the mycelium, isolated in crystalline form and named rapamycin. Rapamycin is mainly active against Candida albicans; minimum inhibitory concentration against ten strains ranged from 0.02 to 0.2 mug/ml. Its apparent activity against Microsporum gypseum and Trichophyton granulosum is lower because of its instability in culture media on prolonged incubation required by these fungi. No activity was observed against gram-positive and gram-negative bacteria. Acute toxicity in mice is low."}
{"id": "PMID:1102509", "title": "Rapamycin (AY-22,989), a new antifungal antibiotic. II. Fermentation, isolation and characterization.", "content": "Rapamycin is a new antifungal antibiotic produced by Streptomyces hygroscopicus NRRL 5491. It was isolated from the mycelium by solvent extraction, purified by silica gel column chromatography and crystallized as a colorless solid which melts at 183 approximately to 185 degrees C and has the empirical formula C56H89NO14. From its characteristic ultraviolet absorption spectrum rapamycin can be classified as a triene. It is highly active against various Candida species, especially Candida albicans. Its activity is compared with that of amphotericin B, candicidin and nystatin.", "contents": "Rapamycin (AY-22,989), a new antifungal antibiotic. II. Fermentation, isolation and characterization. Rapamycin is a new antifungal antibiotic produced by Streptomyces hygroscopicus NRRL 5491. It was isolated from the mycelium by solvent extraction, purified by silica gel column chromatography and crystallized as a colorless solid which melts at 183 approximately to 185 degrees C and has the empirical formula C56H89NO14. From its characteristic ultraviolet absorption spectrum rapamycin can be classified as a triene. It is highly active against various Candida species, especially Candida albicans. Its activity is compared with that of amphotericin B, candicidin and nystatin."}
{"id": "PMID:1102510", "title": "A glyoxalase I inhibitor of a new structural type produced by Streptomyces.", "content": "Many streptomyces strains produced an inhibitor of crude glyoxalase prepared from rat liver which did not inhibit glyoxalase I prepared from yeast. Another inhibitor, C11H14O6, which inhibited glyoxalases prepared from both rat liver and yeast was obtained from a cultured broth of Streptomyces griseosproeus and crystallized. Preincubation of this inhibitor with reuduced glutathione increased its inhibitory activity, which suggested its reaction with reduced glutathione. It showed a strong inhibition of growth of HeLa cells and inhibition of Ehrlich ascites carcinoma by daily injection. It also showed weak inhibition of the solid type of Ehrlich carcinoma and prolonged the survival period of mice inoculated with L-1210 cells.", "contents": "A glyoxalase I inhibitor of a new structural type produced by Streptomyces. Many streptomyces strains produced an inhibitor of crude glyoxalase prepared from rat liver which did not inhibit glyoxalase I prepared from yeast. Another inhibitor, C11H14O6, which inhibited glyoxalases prepared from both rat liver and yeast was obtained from a cultured broth of Streptomyces griseosproeus and crystallized. Preincubation of this inhibitor with reuduced glutathione increased its inhibitory activity, which suggested its reaction with reduced glutathione. It showed a strong inhibition of growth of HeLa cells and inhibition of Ehrlich ascites carcinoma by daily injection. It also showed weak inhibition of the solid type of Ehrlich carcinoma and prolonged the survival period of mice inoculated with L-1210 cells."}
{"id": "PMID:1102511", "title": "Characteristics of two broad spectrum antibiotics produced by Mycoplasma sp. RP III.", "content": "Two extracellular antibiotics produced by Mycoplasma sp. RP III growing in serum-containing media have been purified by solvent extraction and ion-exchange chromatography. Factor I inhibits Gram-positive and Gram-negative bacteria in vitro and a crude preparation protected mice from Pseudomonas and Straphylococcus infections; it is of low cytotoxicity. Factor II, a lipid, inhibits Gram-positive and Gram-negative bacteria, Candida species, and is as cytotoxic as the actinomycins.", "contents": "Characteristics of two broad spectrum antibiotics produced by Mycoplasma sp. RP III. Two extracellular antibiotics produced by Mycoplasma sp. RP III growing in serum-containing media have been purified by solvent extraction and ion-exchange chromatography. Factor I inhibits Gram-positive and Gram-negative bacteria in vitro and a crude preparation protected mice from Pseudomonas and Straphylococcus infections; it is of low cytotoxicity. Factor II, a lipid, inhibits Gram-positive and Gram-negative bacteria, Candida species, and is as cytotoxic as the actinomycins."}
{"id": "PMID:1102513", "title": "Platomycins A and B I Taxonomy of the producing strain and production, isolation and biological properties of platomycins.", "content": "Two new antibiotics, platomycins A and B, belonging to the phleomycin-bleomycin family, were isolated from the culture filtrate of Streptosporangium sp. MK-78. This strain has been identified as a new variety of Streptosporangium violaceochromogenes. Both platomycins A and B are active against a variety of Gram-positive and Gram-negative bacteria, and also inhibit solid Sarcoma 180 and Ehrlich ascites carcinoma.", "contents": "Platomycins A and B I Taxonomy of the producing strain and production, isolation and biological properties of platomycins. Two new antibiotics, platomycins A and B, belonging to the phleomycin-bleomycin family, were isolated from the culture filtrate of Streptosporangium sp. MK-78. This strain has been identified as a new variety of Streptosporangium violaceochromogenes. Both platomycins A and B are active against a variety of Gram-positive and Gram-negative bacteria, and also inhibit solid Sarcoma 180 and Ehrlich ascites carcinoma."}
{"id": "PMID:1102514", "title": "Transduction of drug resistance to tetracycline, chloramphenicol, macrolides, lincomycin and clindamycin with phages induced from Streptococcus pyogenes.", "content": "Strains of Streptococcus pyogenes isolated from pediatric patients with acute infections which were resistant to one or more of the antibiotics, tetracycline (TC), chloramphenicol (CP), macrolide antibiotics (erythromycin, kitasamycin, oleandomycin, josamycin), lincomycin (LCM) and clindamycin (CLM), were used for transduction of drug resistance. These drug-resistant strains were treated with mitomycin C to induce phages and transduction of drug resistance was attempted by means of phages so induced. It was found that transduction of resistance to the above antibiotics was possible. The transductants obtained on TC-containing selective agar plate were resistant to TC alone while those produced on CP- or erythromycin (EM)-containing selective agar plate were resistant to CP, macrolide antibiotics (Mac), LCM and CLM. From this finding, it was inferred that transduction of resistance to TC, CP, Mac, LCM and CLM via phages occurred in two different patterns, i.e., transfer of resistance to TC alone and that of resistance to CP, Mac, LCM and CLM. All of the transductants obtained were found to belong to group A. In T-typing, they were of the same T-12 type as the donor and recipient strains in a majority of cases though some were not typable.", "contents": "Transduction of drug resistance to tetracycline, chloramphenicol, macrolides, lincomycin and clindamycin with phages induced from Streptococcus pyogenes. Strains of Streptococcus pyogenes isolated from pediatric patients with acute infections which were resistant to one or more of the antibiotics, tetracycline (TC), chloramphenicol (CP), macrolide antibiotics (erythromycin, kitasamycin, oleandomycin, josamycin), lincomycin (LCM) and clindamycin (CLM), were used for transduction of drug resistance. These drug-resistant strains were treated with mitomycin C to induce phages and transduction of drug resistance was attempted by means of phages so induced. It was found that transduction of resistance to the above antibiotics was possible. The transductants obtained on TC-containing selective agar plate were resistant to TC alone while those produced on CP- or erythromycin (EM)-containing selective agar plate were resistant to CP, macrolide antibiotics (Mac), LCM and CLM. From this finding, it was inferred that transduction of resistance to TC, CP, Mac, LCM and CLM via phages occurred in two different patterns, i.e., transfer of resistance to TC alone and that of resistance to CP, Mac, LCM and CLM. All of the transductants obtained were found to belong to group A. In T-typing, they were of the same T-12 type as the donor and recipient strains in a majority of cases though some were not typable."}
{"id": "PMID:1102518", "title": "Distribution volumes of [131I]albumin, [14C]sucrose, and 36Cl in sheep lung.", "content": "We measured the steady-state volumes of distribution for radioactive chloride, sucrose, and albumin in the lung of six anesthetized, spen-thorax sheep. We allowed 2 days for [131I]albumin to equilibrate throughout the body, 2 h for the 36Cl, and a 40-min constant infusion for [14C]sucrose before killing the animal. Five minutes before death, we gave [125I]albumin to tag lung plasma volume. We killed the animals by clamping both lung hila; we then removed the lungs and homogenized them. We measured residual red cell and plasma volume, total extravascular lung water, and the extravascular content of the three tracers. The distribution volumes expressed as fractions of blood-free lung weight were: 36Cl equals 0.44, sucrose equals 0.28, and albumin equals 0.07. If the sucrose distribution volume is taken as the best estimate of the lung's extravascular extracellular space, then chloride clearly overestimates the interstitial fluid volume, being either bound or partially intracellular. On the other hand, albumin clearly underestimates the interstitial fluid volume.", "contents": "Distribution volumes of [131I]albumin, [14C]sucrose, and 36Cl in sheep lung. We measured the steady-state volumes of distribution for radioactive chloride, sucrose, and albumin in the lung of six anesthetized, spen-thorax sheep. We allowed 2 days for [131I]albumin to equilibrate throughout the body, 2 h for the 36Cl, and a 40-min constant infusion for [14C]sucrose before killing the animal. Five minutes before death, we gave [125I]albumin to tag lung plasma volume. We killed the animals by clamping both lung hila; we then removed the lungs and homogenized them. We measured residual red cell and plasma volume, total extravascular lung water, and the extravascular content of the three tracers. The distribution volumes expressed as fractions of blood-free lung weight were: 36Cl equals 0.44, sucrose equals 0.28, and albumin equals 0.07. If the sucrose distribution volume is taken as the best estimate of the lung's extravascular extracellular space, then chloride clearly overestimates the interstitial fluid volume, being either bound or partially intracellular. On the other hand, albumin clearly underestimates the interstitial fluid volume."}
{"id": "PMID:1102519", "title": "Rapid estimation of pulmonary extravascular water with an instream catheter.", "content": "An arterial catheter-bearing external conductivity electrodes and a thermistor was used for measurement of lung thermal volume (LTV) by the double-indicator method. Ten milliliters of 3% saline at room temperature were injected, dilution curves measured, and LTV calculated as mean transit time difference, less thermistor time constant, times cardiac output (CO). Comparisons were made, in dogs, between LTV, pulmonary extravascular lung water with Evans blue and tritiated water (PEVWtho), and weighed lung water (WLW). Pulmonary edema was induced with dextran and epinephrine. CO was measured by thermodilution in both the pulmonary artery (PA) and aorta (AO) and dye dilution in the AO. CO from dye dilution was compared with thermodilution (aortic detection) to detect irreversible loss of thermal indicator. Comparisons showed good correspondence of dye and thermal curves (Y = 0.91X - 0.16 1/min; r = 0.93). LTV is about 120% of WLW in near normal lungs, 90% of WLW in extreme edema. PEVWtho was 60-70% WLW.", "contents": "Rapid estimation of pulmonary extravascular water with an instream catheter. An arterial catheter-bearing external conductivity electrodes and a thermistor was used for measurement of lung thermal volume (LTV) by the double-indicator method. Ten milliliters of 3% saline at room temperature were injected, dilution curves measured, and LTV calculated as mean transit time difference, less thermistor time constant, times cardiac output (CO). Comparisons were made, in dogs, between LTV, pulmonary extravascular lung water with Evans blue and tritiated water (PEVWtho), and weighed lung water (WLW). Pulmonary edema was induced with dextran and epinephrine. CO was measured by thermodilution in both the pulmonary artery (PA) and aorta (AO) and dye dilution in the AO. CO from dye dilution was compared with thermodilution (aortic detection) to detect irreversible loss of thermal indicator. Comparisons showed good correspondence of dye and thermal curves (Y = 0.91X - 0.16 1/min; r = 0.93). LTV is about 120% of WLW in near normal lungs, 90% of WLW in extreme edema. PEVWtho was 60-70% WLW."}
{"id": "PMID:1102522", "title": "Mycoplasma hominis: growth, reproduction, and isolation of small viable cells.", "content": "Improved methods for studying the growth of Mycoplasma hominis (ATCC 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. Cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. Similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefore seems characteristic of the species. The pleomorphic populations contain small forms having diameters within the 100- to 250-nm size range reported for \"elementary bodies.\" Such forms were isolated from this strain of M. hominis by sequential filtration using gravity alone, after cell aggregates were dispersed by Pronase treatment. Of the small bodies which traversed membranes of 220-nm pore size, a negligible number grew in liquid or on solid media, suggesting that these were not essential reproductive units in a life cycle, but involution forms due to growth in an altered environment.", "contents": "Mycoplasma hominis: growth, reproduction, and isolation of small viable cells. Improved methods for studying the growth of Mycoplasma hominis (ATCC 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. Cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. Similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefore seems characteristic of the species. The pleomorphic populations contain small forms having diameters within the 100- to 250-nm size range reported for \"elementary bodies.\" Such forms were isolated from this strain of M. hominis by sequential filtration using gravity alone, after cell aggregates were dispersed by Pronase treatment. Of the small bodies which traversed membranes of 220-nm pore size, a negligible number grew in liquid or on solid media, suggesting that these were not essential reproductive units in a life cycle, but involution forms due to growth in an altered environment."}
{"id": "PMID:1102523", "title": "Yeast sterol esters and their relationship to the growth of yeast.", "content": "Variation in the percentage of sterols esterified to long-chain fatty acids during cellular growth has been examined. Under all conditions, a constant percentage of sterol esters was maintained during exponential growth. This maintenance level was found to vary with different growth conditions. A sharp increase in the rate of esterification was observed upon entry of the culture into the stationary growth phase. The minor cellular sterol components were found to accumulate after this period of rapid sterol ester synthesis, with a relative decrease in the size of the ergosterol pool. Evidence is presented that sterol esters of ergosterol precursors are unable to be metabolized to ergosterol. Once esterified, the fatty acids do not appear to be scavenged during starvation conditions.", "contents": "Yeast sterol esters and their relationship to the growth of yeast. Variation in the percentage of sterols esterified to long-chain fatty acids during cellular growth has been examined. Under all conditions, a constant percentage of sterol esters was maintained during exponential growth. This maintenance level was found to vary with different growth conditions. A sharp increase in the rate of esterification was observed upon entry of the culture into the stationary growth phase. The minor cellular sterol components were found to accumulate after this period of rapid sterol ester synthesis, with a relative decrease in the size of the ergosterol pool. Evidence is presented that sterol esters of ergosterol precursors are unable to be metabolized to ergosterol. Once esterified, the fatty acids do not appear to be scavenged during starvation conditions."}
{"id": "PMID:1102524", "title": "Inhibition of cell division in Escherichia coli K-12 by the R-factor R1 and copy mutants of R1.", "content": "The effect of the copy number of plasmid R1drd-19 on cell division of Escherichia coli K-12 was studied in populations growing as steady-state cultures at different growth rates, the growth rate being varied by use of different carbon sources. The plasmid copy number was also varied by using copy mutants of the R-factor. The mean cell size was larger in populations carrying an R-factor than in R-factorless populations, an effect that was more pronounced at low growth rates and in populations carrying R-factor copy mutants. The increased cell size was due to formation of elongated cells in a fraction of the population and to an increase in the diameter of all cells. The majority of the cells divided at a normal cell length, but the presence of an R-factor caused some cells to elongate, probably by the uncoupling of chromosome replication and cell division. This can be explained as a competition between the chromosome and plasmid replicons for some replication factor(s), presumably acting on both initiation and elongation of replication. The formation of elongated cells was a reversible process, but occasionally some of the elongated cells reached lengths 20 times that of newborn cells. If cell division did not occur at the normal cell size, the septum was not formed until the cell size was four times that of a newborn cell. When an elongated cell divided, it usually formed a polar septum, thus producing a newborn cell of normal cell length. The ability of plasmid-containing cells to omit one cell division but to retain the capacity of dividing one mass doubling later is compatible with a mechanical model for septum formation and cell division.", "contents": "Inhibition of cell division in Escherichia coli K-12 by the R-factor R1 and copy mutants of R1. The effect of the copy number of plasmid R1drd-19 on cell division of Escherichia coli K-12 was studied in populations growing as steady-state cultures at different growth rates, the growth rate being varied by use of different carbon sources. The plasmid copy number was also varied by using copy mutants of the R-factor. The mean cell size was larger in populations carrying an R-factor than in R-factorless populations, an effect that was more pronounced at low growth rates and in populations carrying R-factor copy mutants. The increased cell size was due to formation of elongated cells in a fraction of the population and to an increase in the diameter of all cells. The majority of the cells divided at a normal cell length, but the presence of an R-factor caused some cells to elongate, probably by the uncoupling of chromosome replication and cell division. This can be explained as a competition between the chromosome and plasmid replicons for some replication factor(s), presumably acting on both initiation and elongation of replication. The formation of elongated cells was a reversible process, but occasionally some of the elongated cells reached lengths 20 times that of newborn cells. If cell division did not occur at the normal cell size, the septum was not formed until the cell size was four times that of a newborn cell. When an elongated cell divided, it usually formed a polar septum, thus producing a newborn cell of normal cell length. The ability of plasmid-containing cells to omit one cell division but to retain the capacity of dividing one mass doubling later is compatible with a mechanical model for septum formation and cell division."}
{"id": "PMID:1102525", "title": "Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.", "content": "Plasmid incompatibility was studied in Escherichia coli K-12. By double-antibiotic selection, clones were constructed that carried the two R-factors R1 and R100, both belonging to the compatibility group FII. After release of the selection pressure, each of the two plasmids was lost at the same rate (8% per generation). Mutants of R-factor R1 showing an increased number of copies per chromosome (copy mutants) were tested for their incompatibility towards R-factor R100. The results indicate that plasmid incompatibility is quantitative and not just a qualitative property. All copy mutants studied affected incompatibility, and there were two classes of mutants: one increasing and one decreasing the incompatiblity exerted towards the test plasmid R100. Evidence is presented that incompatibility is related to the mechanisms that control replication. The implications of such a relation on proposed models for control of replication are discussed. The data do not support the hypothesis that plasmid incompatibility is due to competition for a replicational or segregational site.", "contents": "Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12. Plasmid incompatibility was studied in Escherichia coli K-12. By double-antibiotic selection, clones were constructed that carried the two R-factors R1 and R100, both belonging to the compatibility group FII. After release of the selection pressure, each of the two plasmids was lost at the same rate (8% per generation). Mutants of R-factor R1 showing an increased number of copies per chromosome (copy mutants) were tested for their incompatibility towards R-factor R100. The results indicate that plasmid incompatibility is quantitative and not just a qualitative property. All copy mutants studied affected incompatibility, and there were two classes of mutants: one increasing and one decreasing the incompatiblity exerted towards the test plasmid R100. Evidence is presented that incompatibility is related to the mechanisms that control replication. The implications of such a relation on proposed models for control of replication are discussed. The data do not support the hypothesis that plasmid incompatibility is due to competition for a replicational or segregational site."}
{"id": "PMID:1102526", "title": "Further characterization of a non-essential mutator gene in Escherichia coli K-12.", "content": "The properties of mutR, a mutator closely linked to thyA, have been further characterized. We have found that the mutator gene is carried on a specialized transducing phage (lambdapcI857 thyA) generated by the excision of lambdacI857 integrated at a secondary attachment site between lysA and thyA. We present three lines of evidence indicating that mutR is a nonessential gene. (i) Deletions of the mutator can be found amoung survivors of heat induction of lambdacI857 when the phage is integrated between lysA and thyA. (ii) Mutations in mutR can be induced with the frameshift mutagen ICR-191. (iii) An amber mutant in mutR has been found. Viable strains could be made by combining the mutator with polB, polA polR, ligts7, and uvrA mutations. The mutator was still able to increase the spontaneous mutation frequency in these genetic backgrounds. When the reversion patterns of a series of well-characterized trpA mutations were analyzed, the results suggested that mutR is more efficient at causing transitions than transversion mutations.", "contents": "Further characterization of a non-essential mutator gene in Escherichia coli K-12. The properties of mutR, a mutator closely linked to thyA, have been further characterized. We have found that the mutator gene is carried on a specialized transducing phage (lambdapcI857 thyA) generated by the excision of lambdacI857 integrated at a secondary attachment site between lysA and thyA. We present three lines of evidence indicating that mutR is a nonessential gene. (i) Deletions of the mutator can be found amoung survivors of heat induction of lambdacI857 when the phage is integrated between lysA and thyA. (ii) Mutations in mutR can be induced with the frameshift mutagen ICR-191. (iii) An amber mutant in mutR has been found. Viable strains could be made by combining the mutator with polB, polA polR, ligts7, and uvrA mutations. The mutator was still able to increase the spontaneous mutation frequency in these genetic backgrounds. When the reversion patterns of a series of well-characterized trpA mutations were analyzed, the results suggested that mutR is more efficient at causing transitions than transversion mutations."}
{"id": "PMID:1102527", "title": "Chromosome-plasmid interaction in Escherichia coli K-12 carrying a thermosensitive plasmid, Rts1, in autonomous and in integrated states.", "content": "An Hfr strain of Escherichia coli K-12 was obtained by integrative suppression with a thermosensitive plasmid, Rts1. The R plasmid was integrated into the chromosome between rif and thr, and transfer of the chromosome occurred counterclockwise. The thermosensitivity of host cell growth due to the dnaA mutation was markedly but not completely reduced in this integratively suppressed Hfr strain. When the dnaA mutation was removed by transducing the dnaA+ genome to this Hfr, the thermosensitivity of cell growth due to existence of Rts1 was suppressed in contrast to strains carrying it autonomously. Thermosensitivity of cell growth appeared again when the plasmid was detached from the chromosome to exist autonomously. Contrary to the effect on cell growth, the transfer of the chromosome and the plasmid itself and the ability to \"restrict\" T-even phages were still thermosensitive in all of these strains carrying Rts1, irrespective of its state of existence. The detached plasmid as well as the original Rts1 were segregated upon growth at 42 C. These data are discussed in relation to chromosome-plasmid interaction. One of the most important conculusions is that some plasmid genes, related to their replication, are phenotypically suppressed by the chromosome when it is integrated.", "contents": "Chromosome-plasmid interaction in Escherichia coli K-12 carrying a thermosensitive plasmid, Rts1, in autonomous and in integrated states. An Hfr strain of Escherichia coli K-12 was obtained by integrative suppression with a thermosensitive plasmid, Rts1. The R plasmid was integrated into the chromosome between rif and thr, and transfer of the chromosome occurred counterclockwise. The thermosensitivity of host cell growth due to the dnaA mutation was markedly but not completely reduced in this integratively suppressed Hfr strain. When the dnaA mutation was removed by transducing the dnaA+ genome to this Hfr, the thermosensitivity of cell growth due to existence of Rts1 was suppressed in contrast to strains carrying it autonomously. Thermosensitivity of cell growth appeared again when the plasmid was detached from the chromosome to exist autonomously. Contrary to the effect on cell growth, the transfer of the chromosome and the plasmid itself and the ability to \"restrict\" T-even phages were still thermosensitive in all of these strains carrying Rts1, irrespective of its state of existence. The detached plasmid as well as the original Rts1 were segregated upon growth at 42 C. These data are discussed in relation to chromosome-plasmid interaction. One of the most important conculusions is that some plasmid genes, related to their replication, are phenotypically suppressed by the chromosome when it is integrated."}
{"id": "PMID:1102528", "title": "Incorporation of pantothenate into citrate lyase by a pantothenateless mutant of Klebsiella pneumoniae.", "content": "A pantothenate-requiring mutant of Klebsiella pneumoniae was isolated. The mutant showed an absolute dependence on pantothenate for growth. When grown in the presence of [14C]pantothenate, the mutant incorporated [14C]pantothenate into citrate lyase (3.4 mol/mol of enzyme). Analysis of a double-labeled enzyme ([14C]pantothenate and [3H]acetate) by gel electrophoresis in sodium dodecyl sulfate showed that both 3H and 14C were associated solely with the smallest subunit, the acyl carrier protein of citrate lyase.", "contents": "Incorporation of pantothenate into citrate lyase by a pantothenateless mutant of Klebsiella pneumoniae. A pantothenate-requiring mutant of Klebsiella pneumoniae was isolated. The mutant showed an absolute dependence on pantothenate for growth. When grown in the presence of [14C]pantothenate, the mutant incorporated [14C]pantothenate into citrate lyase (3.4 mol/mol of enzyme). Analysis of a double-labeled enzyme ([14C]pantothenate and [3H]acetate) by gel electrophoresis in sodium dodecyl sulfate showed that both 3H and 14C were associated solely with the smallest subunit, the acyl carrier protein of citrate lyase."}
{"id": "PMID:1102529", "title": "Factors affecting the palmitoyl-coenzyme A desaturase of Saccharomyces cerevisiae.", "content": "The activity and stability of the palmitoyl-coenzyme A (CoA) desaturase complex of Saccharomyces cerevisiae was influenced by several factors. Cells, grown nonaerobically and then incubated with glucose, either in air or under N2, showed a marked increase in desaturase activity. Cycloheximide, added during such incubations, prevented the increase in activity, suggesting de novo synthesis. The stability of the desaturase from cells grown nonaerobically was affected by subsequent treatment of the cells; enzyme from freshly harvested cells, or from cells that were then shaken under nitrogen, readily lost activity upon washing or during density gradient analysis, whereas aerated cells, in the presence or absence of glucose, yielded stable enzyme preparations. The loss of activity in nonaerobic preparations could be reversed by adding soluble supernatant from these homogenates and could be prevented by growing the cells in the presence of palmitoleic acid and ergosterol, but not with several other lipids tested.", "contents": "Factors affecting the palmitoyl-coenzyme A desaturase of Saccharomyces cerevisiae. The activity and stability of the palmitoyl-coenzyme A (CoA) desaturase complex of Saccharomyces cerevisiae was influenced by several factors. Cells, grown nonaerobically and then incubated with glucose, either in air or under N2, showed a marked increase in desaturase activity. Cycloheximide, added during such incubations, prevented the increase in activity, suggesting de novo synthesis. The stability of the desaturase from cells grown nonaerobically was affected by subsequent treatment of the cells; enzyme from freshly harvested cells, or from cells that were then shaken under nitrogen, readily lost activity upon washing or during density gradient analysis, whereas aerated cells, in the presence or absence of glucose, yielded stable enzyme preparations. The loss of activity in nonaerobic preparations could be reversed by adding soluble supernatant from these homogenates and could be prevented by growing the cells in the presence of palmitoleic acid and ergosterol, but not with several other lipids tested."}
{"id": "PMID:1102530", "title": "Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine.", "content": "The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic pathway and one enzyme of the arginine biosynthetic pathway were assayed from cells grown under a variety of conditions. Cytidine repressed the synthesis of dihydroorotase (encoded by pyrC), dihydroorotate dehydrogenase (encoded by pyrD), and ornithine transcarbamylase (encoded by argI). Moreover, aspartate transcarbamylase (encoded by pyrB) became further derepressed upon cytidine addition, whereas no change occurred in the levels of the last two enzymes (encoded by pyrE and pyrF) of the pyrimidine pathway. Quantitative nucleotide pool determinations have provided evidence that any individual ribo- or deoxyribonucleoside mono-, di-, or triphosphate of cytosine or uracil is not a repressing metabolite for the pyrimidine biosynthetic enzymes. Other nucleotide derivatives or ratios must be considered.", "contents": "Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine. The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic pathway and one enzyme of the arginine biosynthetic pathway were assayed from cells grown under a variety of conditions. Cytidine repressed the synthesis of dihydroorotase (encoded by pyrC), dihydroorotate dehydrogenase (encoded by pyrD), and ornithine transcarbamylase (encoded by argI). Moreover, aspartate transcarbamylase (encoded by pyrB) became further derepressed upon cytidine addition, whereas no change occurred in the levels of the last two enzymes (encoded by pyrE and pyrF) of the pyrimidine pathway. Quantitative nucleotide pool determinations have provided evidence that any individual ribo- or deoxyribonucleoside mono-, di-, or triphosphate of cytosine or uracil is not a repressing metabolite for the pyrimidine biosynthetic enzymes. Other nucleotide derivatives or ratios must be considered."}
{"id": "PMID:1102531", "title": "Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase.", "content": "Several Escherichia coli K-12 mutants blocked in the synthesis of ornithine decarboxylase (OD) were isolated after transduction for serA+ in a strain (MA197) blocked in agmatine ureohydrolase (AUH) with a mutagenized phage lysate of P1. The new double-polyamine mutants were characterized by an unconditional polyamine dependence; either putrescine or spermidine was required for normal growth. The mutational block was varified by the demonstration of a virtual absence of OD activity in cellular extracts. The mutation, designated speC, was mapped by P1 transduction in several strains and was shown to have a cotransduction frequency of 17.2% with serA. Map order was established as serA speB speC metK. A derivative of one of the OD mutants having wild-type levels of AUH and blocked in OD was utilized along with an OD AUH mutant and an OD+ AUH strain to explore the phenomenon of \"pathway selection\" using growth rate as a parameter. Polyamine pool studies were carried out simultaneously. The results presented here support the hypothesis of pathway selection, implying a preferential utilization of exogenous arginine rather than endogenously produced arginine in polyamine biosynthesis.", "contents": "Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase. Several Escherichia coli K-12 mutants blocked in the synthesis of ornithine decarboxylase (OD) were isolated after transduction for serA+ in a strain (MA197) blocked in agmatine ureohydrolase (AUH) with a mutagenized phage lysate of P1. The new double-polyamine mutants were characterized by an unconditional polyamine dependence; either putrescine or spermidine was required for normal growth. The mutational block was varified by the demonstration of a virtual absence of OD activity in cellular extracts. The mutation, designated speC, was mapped by P1 transduction in several strains and was shown to have a cotransduction frequency of 17.2% with serA. Map order was established as serA speB speC metK. A derivative of one of the OD mutants having wild-type levels of AUH and blocked in OD was utilized along with an OD AUH mutant and an OD+ AUH strain to explore the phenomenon of \"pathway selection\" using growth rate as a parameter. Polyamine pool studies were carried out simultaneously. The results presented here support the hypothesis of pathway selection, implying a preferential utilization of exogenous arginine rather than endogenously produced arginine in polyamine biosynthesis."}
{"id": "PMID:1102532", "title": "Tryptophan synthetase alpha(5.7-S): novel molecular species formed within Escherichia coli.", "content": "A novel molecular species contributes about 5% of the total tryptophan synthetase of Escherichia coli derepressed for the trp operon enzymes. The new species is identified under conditions in which the dissociation of the two nonidentical subunits of the tryptophan synthetase complex is favored. The new species sediments at 5.7S, catalyzes the conversion of indole-3-glycerol phosphate to indole, and has been designated alpha(5.7-S). Although alpha(5.7-S) is not observed in extracts of trpA or trpB mutant strains deficient in the ability to form tryptophan synthetase alpha or beta2 subunits, respectively, a mixture of the two extracts allows the formation of alpha(5.7-S). Similar results are obtained when a homogeneous alpha protein is mixed with an extract of a trpA mutant strain, suggesting that the interaction of alpha and beta2 proteins is obligatory for alpha(5.7-S) formation. One can obtain a beta2 protein preparation that when mixed with a pure alpha protein gives no alpha(5.7-S). Therefore, the interaction of alpha and beta2 proteins alone is not sufficient for the formation of alpha(5.7-S). When a mixture of alpha and beta2 proteins devoid of alpha(5.7-S) is added to extracts of trp deletion mutants, the novel species can be reconstituted in vitro only when deletions are used that carry at least the operator-proximal part of the trpB gene. Therefore, it is concluded that the alpha(5.7-S) species of tryptophan synthetase results from the interaction of the alpha protein, the beta2 protein, and a third component, beta', specified by the deoxyribonucleic acid defined by the end points of two trp deletion mutants.", "contents": "Tryptophan synthetase alpha(5.7-S): novel molecular species formed within Escherichia coli. A novel molecular species contributes about 5% of the total tryptophan synthetase of Escherichia coli derepressed for the trp operon enzymes. The new species is identified under conditions in which the dissociation of the two nonidentical subunits of the tryptophan synthetase complex is favored. The new species sediments at 5.7S, catalyzes the conversion of indole-3-glycerol phosphate to indole, and has been designated alpha(5.7-S). Although alpha(5.7-S) is not observed in extracts of trpA or trpB mutant strains deficient in the ability to form tryptophan synthetase alpha or beta2 subunits, respectively, a mixture of the two extracts allows the formation of alpha(5.7-S). Similar results are obtained when a homogeneous alpha protein is mixed with an extract of a trpA mutant strain, suggesting that the interaction of alpha and beta2 proteins is obligatory for alpha(5.7-S) formation. One can obtain a beta2 protein preparation that when mixed with a pure alpha protein gives no alpha(5.7-S). Therefore, the interaction of alpha and beta2 proteins alone is not sufficient for the formation of alpha(5.7-S). When a mixture of alpha and beta2 proteins devoid of alpha(5.7-S) is added to extracts of trp deletion mutants, the novel species can be reconstituted in vitro only when deletions are used that carry at least the operator-proximal part of the trpB gene. Therefore, it is concluded that the alpha(5.7-S) species of tryptophan synthetase results from the interaction of the alpha protein, the beta2 protein, and a third component, beta', specified by the deoxyribonucleic acid defined by the end points of two trp deletion mutants."}
{"id": "PMID:1102533", "title": "Effect of temperature on the size of Escherichia coli cells.", "content": "The distributions of cell volumes of steady-state Escherichia coli ML30G cultures at various temperatures were measured. For cultures in a minimal medium, the distributions were indistinguishable at several temperatures between 15 and 30 C; at higher temperatures the cells were slightly smaller, and at lower temperatures they were slightly larger. For cultures in a complex medium, the cells were slightly larger at both high and low temperatures of growth. An abrupt change of temperature within the middle range led to a transient change in the distribution of cell volume, suggesting that the size of dividing cells is well regulated. No synchrony of division was induced by a change in temperature.", "contents": "Effect of temperature on the size of Escherichia coli cells. The distributions of cell volumes of steady-state Escherichia coli ML30G cultures at various temperatures were measured. For cultures in a minimal medium, the distributions were indistinguishable at several temperatures between 15 and 30 C; at higher temperatures the cells were slightly smaller, and at lower temperatures they were slightly larger. For cultures in a complex medium, the cells were slightly larger at both high and low temperatures of growth. An abrupt change of temperature within the middle range led to a transient change in the distribution of cell volume, suggesting that the size of dividing cells is well regulated. No synchrony of division was induced by a change in temperature."}
{"id": "PMID:1102534", "title": "Proteinase activities of Saccharomyces cerevisiae during sporulation.", "content": "Sporulation in Saccharomyces cerevisiae occurs in the absence of a exogenous nitrogen source. Thus, the internal amino acid pool and the supply of nitrogen compounds from protein and nucleic acid turnover must be sufficient for new protein synthesis. Since sporulation involves an increased rate of protein turnover, an investigation was conducted of the changes in the specific activity of various proteinases. A minimum of 30% of the vegetative proteins was turned over during the course of sporulation. There was a 10- to 25-fold increase in specific activity of various proteinases, with a maximum activity around 20 h after transfer into the sporulation medium. The increase in activities was due to de novo synthesis since inhibition of protein synthesis by cycloheximide blocks both an increase in proteinase activities and sporulation. There was no increase observed in proteinase activities of nonsporogenic cultures (a and alpha/alpha strains) inoculated into the sporulation medium, suggesting that the increase in proteinase activities is \"sporulation specific\" and not a consequence of step-down conditions. The elution patterns through diethylaminoethyl-Sephadex chromatography of various proteinases extracted from T0 and T18 cells were similar, and no new species was observed.", "contents": "Proteinase activities of Saccharomyces cerevisiae during sporulation. Sporulation in Saccharomyces cerevisiae occurs in the absence of a exogenous nitrogen source. Thus, the internal amino acid pool and the supply of nitrogen compounds from protein and nucleic acid turnover must be sufficient for new protein synthesis. Since sporulation involves an increased rate of protein turnover, an investigation was conducted of the changes in the specific activity of various proteinases. A minimum of 30% of the vegetative proteins was turned over during the course of sporulation. There was a 10- to 25-fold increase in specific activity of various proteinases, with a maximum activity around 20 h after transfer into the sporulation medium. The increase in activities was due to de novo synthesis since inhibition of protein synthesis by cycloheximide blocks both an increase in proteinase activities and sporulation. There was no increase observed in proteinase activities of nonsporogenic cultures (a and alpha/alpha strains) inoculated into the sporulation medium, suggesting that the increase in proteinase activities is \"sporulation specific\" and not a consequence of step-down conditions. The elution patterns through diethylaminoethyl-Sephadex chromatography of various proteinases extracted from T0 and T18 cells were similar, and no new species was observed."}
{"id": "PMID:1102535", "title": "Polypeptide nature of growth requirement in yeast extract for Thermoplasma acidophilum.", "content": "The active component(s) in yeast extract required by Thermoplasma acidophilum for growth is polypeptide in nature. A fraction from yeast extract was isolated and partially characterized as one or more peptides of molecular weight about 1,000 containing 8 to 10 amino acids. Although it was composed largely of basic and dicarboxylic amino acids, only one amino group per molecule was free. The polypeptide(s) appeared to bind avidly to cations. No other organic compounds except glucose were needed by Thermoplasma. Among several hundred known compounds tested, only glutathione plus Fe2+ or Fe3+, clostridial ferredoxin, and spinach ferredoxin elicited any growth response.", "contents": "Polypeptide nature of growth requirement in yeast extract for Thermoplasma acidophilum. The active component(s) in yeast extract required by Thermoplasma acidophilum for growth is polypeptide in nature. A fraction from yeast extract was isolated and partially characterized as one or more peptides of molecular weight about 1,000 containing 8 to 10 amino acids. Although it was composed largely of basic and dicarboxylic amino acids, only one amino group per molecule was free. The polypeptide(s) appeared to bind avidly to cations. No other organic compounds except glucose were needed by Thermoplasma. Among several hundred known compounds tested, only glutathione plus Fe2+ or Fe3+, clostridial ferredoxin, and spinach ferredoxin elicited any growth response."}
{"id": "PMID:1102536", "title": "Enzymatic lesions in methionine mutants of Aspergillus nidulans: role and regulation of an alternative pathway for cysteine and methionine synthesis.", "content": "In Aspergillus nidulans the pathway involving cystathionine formation is the main one for homocysteine synthesis. Mutants lacking cystathionine gamma-synthase or beta-cystathionase are auxotrophs suppressible by: (i) mutations in the main pathway of cysteine synthesis (cysA1, cysB1, and cysC1), (ii) mutations causing stimulation of cysteine catabolism (su101), and (iii) mutations in a presumed regulatory gene (suAmeth). A relative shortage of cysteine in the first group of suppressors causes a derepression of homocysteine synthase, the enzyme involved in the alternative pathway of homocysteine synthesis. A similar derepression is observed in the suAmeth strain. Homocysteine synthesized by this pathway serves as precursor for cysteine and methionine synthesis. A mutant with altered homocysteine synthase is a prototroph, indicating that this enzyme is not essential for the fungus.", "contents": "Enzymatic lesions in methionine mutants of Aspergillus nidulans: role and regulation of an alternative pathway for cysteine and methionine synthesis. In Aspergillus nidulans the pathway involving cystathionine formation is the main one for homocysteine synthesis. Mutants lacking cystathionine gamma-synthase or beta-cystathionase are auxotrophs suppressible by: (i) mutations in the main pathway of cysteine synthesis (cysA1, cysB1, and cysC1), (ii) mutations causing stimulation of cysteine catabolism (su101), and (iii) mutations in a presumed regulatory gene (suAmeth). A relative shortage of cysteine in the first group of suppressors causes a derepression of homocysteine synthase, the enzyme involved in the alternative pathway of homocysteine synthesis. A similar derepression is observed in the suAmeth strain. Homocysteine synthesized by this pathway serves as precursor for cysteine and methionine synthesis. A mutant with altered homocysteine synthase is a prototroph, indicating that this enzyme is not essential for the fungus."}
{"id": "PMID:1102537", "title": "Comparison of the cell envelope structure of a lipopolysaccharide-defective (heptose-deficient) strain and a smooth strain of Salmonella typhimurium.", "content": "The cell envelope structure of Salmonella typhimurium LT2, which has a heptose-deficient lipopolysaccharide (LPS), is significantly different from that of an isogenic strain with a normal LPS. The rough strain, when examined by freeze-etching, lacks most surface structures that are routinely present in the smooth strain (surface particles and flagella) and has few transmemberane studs in the cytoplasmic membrane (those present are generally found in aggregates), and the outer membrane cleavage is substantially stronger than that of the smooth strain. These envelope differences were independent of both growth temperature and culture age. Examination of ultrathin sections indicated that the rough strain has an outer membrane which forms a much more defined double-track artifact than the smooth strain. The addition of MgCl2 to the growth medium of the rough strain decreased the extent of outer membrane cleavage, and flagella became evident in freeze-etched preparations. The presence of supplemental MgCl2 in the growth medium, which resulted in these morphological changes in the rough strain, also produced growth at a previously restrictive temperature and a decrease in the leakage of periplasmic enzymes. The smooth strain was unaltered morphologically or physiologically by MgCl2 under identical conditions. It is suggested that the outer membrane of the rough strain is more planar.", "contents": "Comparison of the cell envelope structure of a lipopolysaccharide-defective (heptose-deficient) strain and a smooth strain of Salmonella typhimurium. The cell envelope structure of Salmonella typhimurium LT2, which has a heptose-deficient lipopolysaccharide (LPS), is significantly different from that of an isogenic strain with a normal LPS. The rough strain, when examined by freeze-etching, lacks most surface structures that are routinely present in the smooth strain (surface particles and flagella) and has few transmemberane studs in the cytoplasmic membrane (those present are generally found in aggregates), and the outer membrane cleavage is substantially stronger than that of the smooth strain. These envelope differences were independent of both growth temperature and culture age. Examination of ultrathin sections indicated that the rough strain has an outer membrane which forms a much more defined double-track artifact than the smooth strain. The addition of MgCl2 to the growth medium of the rough strain decreased the extent of outer membrane cleavage, and flagella became evident in freeze-etched preparations. The presence of supplemental MgCl2 in the growth medium, which resulted in these morphological changes in the rough strain, also produced growth at a previously restrictive temperature and a decrease in the leakage of periplasmic enzymes. The smooth strain was unaltered morphologically or physiologically by MgCl2 under identical conditions. It is suggested that the outer membrane of the rough strain is more planar."}
{"id": "PMID:1102538", "title": "Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants.", "content": "The outer membrane layer of the cell wall was isolated from wild-type Salmonella typhimurium LT2 as well as from its mutants producing lipopolysaccharides with shorter saccharide chains. Chemical analysis of these preparations indicated the following. (i) The number of lipopolysaccharide molecules per unit area was constant, regardless of the length of the saccharide side chain in lipopolysaccharide. (ii) In contrast, in \"deep rough\" (Rd or Re) mutants producing the lipopolysaccharides with very short saccharide chains, the amount of outer membrane protein per unit surface area decreased to about 60% of the value in the wild type. (iii) In the wild type, the amount of phospholipids is slightly less than what is needed to cover one side of the membrane as a monolayer. In comparison with the wild type, the outer membrane of Rd and Re mutants contains about 70% more phospholipids, which therefore must be distributed in both the outer and inner leaflets of the membrane. Freeze-fracture studies showed that the outer membrane of Re mutants were easily fractured, but fracture became increasingly difficult in strains producing lipopolysaccharides with longer side chains. The convex fracture face was always nearly smooth, but the concave fracture face or the outer half of the membrane was densely covered with particles 8 to 10 nm in diameter. The density of particles was decreased in Re mutants to the same extent as the reduction in proteins, suggesting the largely proteinaceous nature of particles. A model for the supramolecular structure of the outer membrane is presented on the basis of these and other results.", "contents": "Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants. The outer membrane layer of the cell wall was isolated from wild-type Salmonella typhimurium LT2 as well as from its mutants producing lipopolysaccharides with shorter saccharide chains. Chemical analysis of these preparations indicated the following. (i) The number of lipopolysaccharide molecules per unit area was constant, regardless of the length of the saccharide side chain in lipopolysaccharide. (ii) In contrast, in \"deep rough\" (Rd or Re) mutants producing the lipopolysaccharides with very short saccharide chains, the amount of outer membrane protein per unit surface area decreased to about 60% of the value in the wild type. (iii) In the wild type, the amount of phospholipids is slightly less than what is needed to cover one side of the membrane as a monolayer. In comparison with the wild type, the outer membrane of Rd and Re mutants contains about 70% more phospholipids, which therefore must be distributed in both the outer and inner leaflets of the membrane. Freeze-fracture studies showed that the outer membrane of Re mutants were easily fractured, but fracture became increasingly difficult in strains producing lipopolysaccharides with longer side chains. The convex fracture face was always nearly smooth, but the concave fracture face or the outer half of the membrane was densely covered with particles 8 to 10 nm in diameter. The density of particles was decreased in Re mutants to the same extent as the reduction in proteins, suggesting the largely proteinaceous nature of particles. A model for the supramolecular structure of the outer membrane is presented on the basis of these and other results."}
{"id": "PMID:1102539", "title": "On the processive mechanism of Escherichia coli DNA polymerase I.", "content": "A procedure has been developed to assess whether polymerization of nucleotides by DNA polymerases is processive, that is, whether a succession of polymerization steps occurs without release of the enzyme from the template. The method involves measurement of the ratio of deoxyguanylate to deocycytidylate incorporated in the course of replicating a segment of the right hand cohesive end of phage lambaDNA with the sequence 5'-G-G-G-C-G-G-C-G-3'. In the case of the Escherichia coli DNA polymerase I, each enzyme molecule completes synthesis of the sequence before dissociation occurs. Furthermore, at both 6 and 22 degrees, the polymerase remains bound to the lambdaDNA template after synthesis has completed. Template challenge experiments, in which the polymerase is allowed to begin synthesis in the presence of a molar excess of lambdaDNA before addition of a very large excess of calf thymus DNA, show that under the conditions used, productive binding of polymerase to lambdaDNA is a slow process requiring 1 to 2 hours. After synthesis has been completed, polymerase remains bound to the lambdaDNA in spite of the availability of new primer termini. The association, polymerization, and dissociation rates measured in these experiments suggest that the polymerization reaction catalyzed by DNA polymerase I is processive, and that hundreds of nucleotides may be polymerized between each association and dissociation.", "contents": "On the processive mechanism of Escherichia coli DNA polymerase I. A procedure has been developed to assess whether polymerization of nucleotides by DNA polymerases is processive, that is, whether a succession of polymerization steps occurs without release of the enzyme from the template. The method involves measurement of the ratio of deoxyguanylate to deocycytidylate incorporated in the course of replicating a segment of the right hand cohesive end of phage lambaDNA with the sequence 5'-G-G-G-C-G-G-C-G-3'. In the case of the Escherichia coli DNA polymerase I, each enzyme molecule completes synthesis of the sequence before dissociation occurs. Furthermore, at both 6 and 22 degrees, the polymerase remains bound to the lambdaDNA template after synthesis has completed. Template challenge experiments, in which the polymerase is allowed to begin synthesis in the presence of a molar excess of lambdaDNA before addition of a very large excess of calf thymus DNA, show that under the conditions used, productive binding of polymerase to lambdaDNA is a slow process requiring 1 to 2 hours. After synthesis has been completed, polymerase remains bound to the lambdaDNA in spite of the availability of new primer termini. The association, polymerization, and dissociation rates measured in these experiments suggest that the polymerization reaction catalyzed by DNA polymerase I is processive, and that hundreds of nucleotides may be polymerized between each association and dissociation."}
{"id": "PMID:1102540", "title": "Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I.", "content": "The kinetic properties of Escherichia coli DNA polymerase I were simplified to those of a 1 deoxynucleotide substrate reaction by the use of polynucleotide templates. With poly(dA)-oligo(dT) as the template-primer complex, Mg2+ decreases the Km of the substrate dTTP but has little or no effect on the Km of the substrate Mg-dTTP, suggesting that multiple pathways involving the binding of Mg2+, dTTP, and Mg-dTTP are operative in forming the active complex. The Km of free Mg2+, extrapolated to zero concentration of substrate (830 = 62 muM), agrees within a factor of 2 with the dissociation constant of magnesium from 4 +/- 1 sites on the enzyme determined previously by binding studies (Slater, J.P., Tamir, I., Loeb, L.A., and Mildvan, A.S. (1972) J. Biol. Chem. 247, 6784-6794). The maximal turnover number with poly(dA) as template is 5.7 +/- 0.7 s-1. Changing the nature of the base in the polydeoxynucleotide template alters the maximal rate of polydeoxynucleotide synthesis by an overall factor of 31 with poly(dC) is greater than poly(dT) is greater than poly(dA) is greater than poly(dG), indicating that pyrimidine templates are copied faster than purine templates. Changing the sugar structure from poly(dA) to poly(rA) causes a 3-fold increase in the rate of template copying. A study of the kinetic effects of all noncomplementary deoxynucleotides with all deoxynucleotide templates, as well as with poly(rA)-oligo(dT), yields complex patterns of activation and inhibition requiring from 1 to 2 additional binding sites for the noncomplementary nucleotides. The kinetically determined affinities of the active site of the enzyme-template-primer complex for the complementary free nucleotide (as measured by Km) generally exceed those for the noncomplementary neuclotides (as measured by KI slope) by 1 or more than 3 orders of magnitude.", "contents": "Kinetic analysis of Escherichia coli deoxyribonucleic acid polymerase I. The kinetic properties of Escherichia coli DNA polymerase I were simplified to those of a 1 deoxynucleotide substrate reaction by the use of polynucleotide templates. With poly(dA)-oligo(dT) as the template-primer complex, Mg2+ decreases the Km of the substrate dTTP but has little or no effect on the Km of the substrate Mg-dTTP, suggesting that multiple pathways involving the binding of Mg2+, dTTP, and Mg-dTTP are operative in forming the active complex. The Km of free Mg2+, extrapolated to zero concentration of substrate (830 = 62 muM), agrees within a factor of 2 with the dissociation constant of magnesium from 4 +/- 1 sites on the enzyme determined previously by binding studies (Slater, J.P., Tamir, I., Loeb, L.A., and Mildvan, A.S. (1972) J. Biol. Chem. 247, 6784-6794). The maximal turnover number with poly(dA) as template is 5.7 +/- 0.7 s-1. Changing the nature of the base in the polydeoxynucleotide template alters the maximal rate of polydeoxynucleotide synthesis by an overall factor of 31 with poly(dC) is greater than poly(dT) is greater than poly(dA) is greater than poly(dG), indicating that pyrimidine templates are copied faster than purine templates. Changing the sugar structure from poly(dA) to poly(rA) causes a 3-fold increase in the rate of template copying. A study of the kinetic effects of all noncomplementary deoxynucleotides with all deoxynucleotide templates, as well as with poly(rA)-oligo(dT), yields complex patterns of activation and inhibition requiring from 1 to 2 additional binding sites for the noncomplementary nucleotides. The kinetically determined affinities of the active site of the enzyme-template-primer complex for the complementary free nucleotide (as measured by Km) generally exceed those for the noncomplementary neuclotides (as measured by KI slope) by 1 or more than 3 orders of magnitude."}
{"id": "PMID:1102541", "title": "Inhibition of growth of microbial mutants by trans-octadecenoates.", "content": "A series of positional isomers of trans-octadecenoic acid were tested for their ability to support growth of microbial mutants that could not synthesize unsaturated fatty acids. The bacterial strain used (a variant of Escherichia coli 30E) grew with supplements of the trans isomers only at high temperatures (38 degrees) and with acids containing the trans-ethylenic bond between carbon atoms 8 through 13. The yeast mutant (Saccharomyces cerevisiae KD46) grew only with the 9-trans-octadecenoate giving cell yields about one-fifth those obtained with oleate. Although the trans isomers had little effect on the growth of the bacteria in the presence of oleate, they inhibited the growth of yeast with oleate. Inhibition was strongest for the 4, 6, 7, 11, and 12 isomers, almost negligible for the 8 isomer and of differing intermediate degrees for the others. The inhibitory effects had no correlation with the melting points of the acids and appeared to reflect selective action(s) on the metabolism of the cell. When the net yield of the yeast cultures with oleate was lowered by the effect of added trans acids, there was a marked accumulation of triglycerides and nonesterified acids in the cells. The marked increase in triglyceride content while phospholipid per cell remained relatively constant suggest that trans acids, in addition to forming inadequate membrane lipids, may also interfere with a basic control point in lipid metabolism.", "contents": "Inhibition of growth of microbial mutants by trans-octadecenoates. A series of positional isomers of trans-octadecenoic acid were tested for their ability to support growth of microbial mutants that could not synthesize unsaturated fatty acids. The bacterial strain used (a variant of Escherichia coli 30E) grew with supplements of the trans isomers only at high temperatures (38 degrees) and with acids containing the trans-ethylenic bond between carbon atoms 8 through 13. The yeast mutant (Saccharomyces cerevisiae KD46) grew only with the 9-trans-octadecenoate giving cell yields about one-fifth those obtained with oleate. Although the trans isomers had little effect on the growth of the bacteria in the presence of oleate, they inhibited the growth of yeast with oleate. Inhibition was strongest for the 4, 6, 7, 11, and 12 isomers, almost negligible for the 8 isomer and of differing intermediate degrees for the others. The inhibitory effects had no correlation with the melting points of the acids and appeared to reflect selective action(s) on the metabolism of the cell. When the net yield of the yeast cultures with oleate was lowered by the effect of added trans acids, there was a marked accumulation of triglycerides and nonesterified acids in the cells. The marked increase in triglyceride content while phospholipid per cell remained relatively constant suggest that trans acids, in addition to forming inadequate membrane lipids, may also interfere with a basic control point in lipid metabolism."}
{"id": "PMID:1102543", "title": "Relaxation complexes of plasmid DNA and protein. I. Strand-specific association of protein and DNA in the relaxed complexes of plasmids ColE1 and ColE2.", "content": "The ColE1 and ColE2 relaxation complexes of supercoiled DNA and protein were purified from Escherichia coli cells. Protein remains associated with the open circular DNA of these complexes after induction of relaxation with sodium dodecyl sulfate. The protein is associated specifically with the strand that possesses a site-specific break in the Co1E1 and ColE2 relaxed complexes. This protein remains associated with the DNA after centrifugation of the relaxed complex in a neutral or alkaline (pH 12.5) cesium chloride gradient or treatment with 8 M urea, 2 M NaSCN, 2M LiCl, 0.2 M sodium acetate, pH 4.6, and 70% formamide at 60 degrees.", "contents": "Relaxation complexes of plasmid DNA and protein. I. Strand-specific association of protein and DNA in the relaxed complexes of plasmids ColE1 and ColE2. The ColE1 and ColE2 relaxation complexes of supercoiled DNA and protein were purified from Escherichia coli cells. Protein remains associated with the open circular DNA of these complexes after induction of relaxation with sodium dodecyl sulfate. The protein is associated specifically with the strand that possesses a site-specific break in the Co1E1 and ColE2 relaxed complexes. This protein remains associated with the DNA after centrifugation of the relaxed complex in a neutral or alkaline (pH 12.5) cesium chloride gradient or treatment with 8 M urea, 2 M NaSCN, 2M LiCl, 0.2 M sodium acetate, pH 4.6, and 70% formamide at 60 degrees."}
{"id": "PMID:1102544", "title": "Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.", "content": "The proteins of the DNA-protein relaxation complex of plasmid ColE1 were labeled with [3H]leucine by growth of ColE1 containing Escherichia coli cells in the presence of this radioactive labeled amino acid. Three major [3H]leucine-labeled proteins are found associated with the supercoiled DNA in the ColE1 relaxation complex. The molecular weights of these proteins, determined by sodium dodecyl sulfate-acrylamide gel electrophoresis, are 60,000, 16,000, and 11,000, respectively. Induction of relaxation of the supercoiled DNA by treatment of the complex with sodium dodecyl sulfate results in a dissociation of the two smaller proteins from the DNA. The 60,000 protein, however, remains associated specifically with the nicked strand of the open circular DNA. The strand-specific association of this protein with the relaxed DNA resists heat denaturation of the DNA, sedimentation through an alkaline (pH 12.5) sucrose gradient, and centrifugation to equilibrium in an alkaline (pH 12.5) CsCl gradient.", "contents": "Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1. The proteins of the DNA-protein relaxation complex of plasmid ColE1 were labeled with [3H]leucine by growth of ColE1 containing Escherichia coli cells in the presence of this radioactive labeled amino acid. Three major [3H]leucine-labeled proteins are found associated with the supercoiled DNA in the ColE1 relaxation complex. The molecular weights of these proteins, determined by sodium dodecyl sulfate-acrylamide gel electrophoresis, are 60,000, 16,000, and 11,000, respectively. Induction of relaxation of the supercoiled DNA by treatment of the complex with sodium dodecyl sulfate results in a dissociation of the two smaller proteins from the DNA. The 60,000 protein, however, remains associated specifically with the nicked strand of the open circular DNA. The strand-specific association of this protein with the relaxed DNA resists heat denaturation of the DNA, sedimentation through an alkaline (pH 12.5) sucrose gradient, and centrifugation to equilibrium in an alkaline (pH 12.5) CsCl gradient."}
{"id": "PMID:1102545", "title": "Relaxation complexes of poasmid DNA and protein. III. Association of protein with the 5' terminus of the broken DNA strand in the relaxed complex of plasmid ColE1.", "content": "The location of the protein in the open circular DNA form of the ColE1 DNA-protein relaxation complex, induced by treatment with sodium dodecyl sulfate, has been studied using several enzymes of DNA metabolism. Escherichia coli exonucleases I and III are able to degrade extensively the nicked strand of the relaxed complex from the 3' end. DNA polymerase I can initiate synthesis using the relaxed complex as template-primer and specifically extends the 3' end of the nicked strand. The 5' end of the sodium dodecyl sulfate-relaxed complex, however, is blocked to the 5'-3' hydrolitic action T7 exonuclease. This block remains after trypsin treatment of the sodium dodecyl sulfate-relaxed complex but is removed by Pronase treatment. T4 DNA ligase is unable to seal either the sodium dodecyl sulfate-relaxed complex or the Pronase-treated relaxed complex even after pretreatment of the relaxed complex with T4 DNA polymerase and polynucleotide kinase. However, pretreatment with DNA polymerase I and the four deoxyribonucleoside triphosphates facilitates ligase closure of the Pronase-treated relaxed complex but not the sodium dodecyl sulfate-relaxed complex. These studies indicate that the protein in the relaxed ColE1 complex is located at or near the 5' end of the nicked strand.", "contents": "Relaxation complexes of poasmid DNA and protein. III. Association of protein with the 5' terminus of the broken DNA strand in the relaxed complex of plasmid ColE1. The location of the protein in the open circular DNA form of the ColE1 DNA-protein relaxation complex, induced by treatment with sodium dodecyl sulfate, has been studied using several enzymes of DNA metabolism. Escherichia coli exonucleases I and III are able to degrade extensively the nicked strand of the relaxed complex from the 3' end. DNA polymerase I can initiate synthesis using the relaxed complex as template-primer and specifically extends the 3' end of the nicked strand. The 5' end of the sodium dodecyl sulfate-relaxed complex, however, is blocked to the 5'-3' hydrolitic action T7 exonuclease. This block remains after trypsin treatment of the sodium dodecyl sulfate-relaxed complex but is removed by Pronase treatment. T4 DNA ligase is unable to seal either the sodium dodecyl sulfate-relaxed complex or the Pronase-treated relaxed complex even after pretreatment of the relaxed complex with T4 DNA polymerase and polynucleotide kinase. However, pretreatment with DNA polymerase I and the four deoxyribonucleoside triphosphates facilitates ligase closure of the Pronase-treated relaxed complex but not the sodium dodecyl sulfate-relaxed complex. These studies indicate that the protein in the relaxed ColE1 complex is located at or near the 5' end of the nicked strand."}
{"id": "PMID:1102550", "title": "The development of a computer-assisted search for anomalous compounds (CASAC).", "content": "An automated system for the recognition of anomalies in multi-compound mixtures is described. The mixtures to be investigated are injected into a combined gas chromatograph-mass spectrometer and low resolution spectra are acquired by repetitive scanning. Using an on-line computer, all of these spectra are compared with a pre-recorded file of spectra obtained by identical analyses of a \"normal\" mixture. The matching procedure has been designed to allow for differences in retention times. The CASAC program calculates and plots the degree of coincidence and in this way determines whether the sample spectrum contains fewer or more fragments than the corresponding library spectrum. The system has been applied to studies on pathological urine samples.", "contents": "The development of a computer-assisted search for anomalous compounds (CASAC). An automated system for the recognition of anomalies in multi-compound mixtures is described. The mixtures to be investigated are injected into a combined gas chromatograph-mass spectrometer and low resolution spectra are acquired by repetitive scanning. Using an on-line computer, all of these spectra are compared with a pre-recorded file of spectra obtained by identical analyses of a \"normal\" mixture. The matching procedure has been designed to allow for differences in retention times. The CASAC program calculates and plots the degree of coincidence and in this way determines whether the sample spectrum contains fewer or more fragments than the corresponding library spectrum. The system has been applied to studies on pathological urine samples."}
{"id": "PMID:1102551", "title": "The effects of estrogen and progesterone on the functional capacity of the gonadotrophs.", "content": "The functional capacity of the gonadotrophs under the influence of exogenous estrogen and progesterone was assessed by repeated stimulation with submaximal doses of LH-releasing factor (LRF) (10 mug at 2-h intervals) of subjects during the early follicular phase of the cycle and of hypogonadal women. The initial increment of peak serum LH and FSH concentrations after the first administration of LRF, was used to describe the pituitary sensitivity, and the integrated release during the 10 hours of LRF-stimulated pulses was utilized to approximate the pituitary gonadotropin reserve. During the early follicular phase, response to LRF stimulations was relatively stable with corresponding release of LH and FSH. An augmentation of sensitivity, as well as the reserve, for both LH and FSH was elicited by an incremental change in circulating estradiol levels, a change imposed by daily administration of estradiol benzoate for 4 days during the early follicular phase. Under the same conditions, the addition of progesterone (10 mg, im) at the end of the estradiol benzoate treatment induced a marked amplification of the estrogen-augmented pituitary gonadotropin sensitivity and reserve. The pituitary sensitivity, relatively higher than the reserve in hypogonadal subjects, was reversed by the administration of ethinyl estradiol (20-50 mug/day) for 7 days. These data indicate that the functional capacity of the gondotrophs is profoundly modulated by estrogen through relative changes in pituitary sensitivity and reserve, and that progesterone in low doses exhibited an amplifying effect on estrogen-primed gonadotrophs in both the pituitary sensitivity and the reserve.", "contents": "The effects of estrogen and progesterone on the functional capacity of the gonadotrophs. The functional capacity of the gonadotrophs under the influence of exogenous estrogen and progesterone was assessed by repeated stimulation with submaximal doses of LH-releasing factor (LRF) (10 mug at 2-h intervals) of subjects during the early follicular phase of the cycle and of hypogonadal women. The initial increment of peak serum LH and FSH concentrations after the first administration of LRF, was used to describe the pituitary sensitivity, and the integrated release during the 10 hours of LRF-stimulated pulses was utilized to approximate the pituitary gonadotropin reserve. During the early follicular phase, response to LRF stimulations was relatively stable with corresponding release of LH and FSH. An augmentation of sensitivity, as well as the reserve, for both LH and FSH was elicited by an incremental change in circulating estradiol levels, a change imposed by daily administration of estradiol benzoate for 4 days during the early follicular phase. Under the same conditions, the addition of progesterone (10 mg, im) at the end of the estradiol benzoate treatment induced a marked amplification of the estrogen-augmented pituitary gonadotropin sensitivity and reserve. The pituitary sensitivity, relatively higher than the reserve in hypogonadal subjects, was reversed by the administration of ethinyl estradiol (20-50 mug/day) for 7 days. These data indicate that the functional capacity of the gondotrophs is profoundly modulated by estrogen through relative changes in pituitary sensitivity and reserve, and that progesterone in low doses exhibited an amplifying effect on estrogen-primed gonadotrophs in both the pituitary sensitivity and the reserve."}
{"id": "PMID:1102552", "title": "Cell contacts in human islets of Langerhans.", "content": "The freeze-fracturing technique was applied to fresh human islets of Langerhans. With this technique, the inside of cellular membranes was revealed, and specific membrane differentiations, hitherto unknown, were observed in the plasma membrane of the endocrine cells. The specific membrane differentiations represent two types of intercellular junctions, namely the tight junction, which determines a closure of the extracellular space and the gap junction which allows molecules and ions to diffuse from one cell to another (sharing the gap junction) without leaking in the extracellular space (intercellular coupling). The presence of such junctions may be important for the secretory behavior of the cells within the islet.", "contents": "Cell contacts in human islets of Langerhans. The freeze-fracturing technique was applied to fresh human islets of Langerhans. With this technique, the inside of cellular membranes was revealed, and specific membrane differentiations, hitherto unknown, were observed in the plasma membrane of the endocrine cells. The specific membrane differentiations represent two types of intercellular junctions, namely the tight junction, which determines a closure of the extracellular space and the gap junction which allows molecules and ions to diffuse from one cell to another (sharing the gap junction) without leaking in the extracellular space (intercellular coupling). The presence of such junctions may be important for the secretory behavior of the cells within the islet."}
{"id": "PMID:1102549", "title": "A study of streptokinase therapy in acute pulmonary embolism.", "content": "A study of Streptokinase therapy in major pulmonary emboli is reported and attention is drawn to some of the factors which can influence the results of this as yet controversial therapy.", "contents": "A study of streptokinase therapy in acute pulmonary embolism. A study of Streptokinase therapy in major pulmonary emboli is reported and attention is drawn to some of the factors which can influence the results of this as yet controversial therapy."}
{"id": "PMID:1102553", "title": "Serum LH and FSH response in four-hour infusions of luteinizing hormone-releasing hormone in normal men, Sertoli cell only syndrome, and Klinefelter's syndrome.", "content": "Constant intravenous infusion of 1 mug/min of luteinizing hormone-releasing hormone (LHRH) for 4 hours into normal men produced a biphasic pattern of LH secretion. The initial LH peak occurred 15-45 minutes after the commencement of the infusion, following which LH levels plateaued or declined, subsequently rising as the infusion continued. No biphasic pattern of secretion could be identified for FSH. During the infusion, no consistent testosterone rise could be identified despite significant elevation of LH levels. Similar infusions of LHRH in men with Sertoli cell only syndrome produced exaggerated responses in terms of both LH and FSH. In contrast, a single intravenous bolus of 100 mug/ LHRH produced an exaggerated response in only one of the 3 men studied, suggesting that the prolonged infusion may be a more discerning dynamic test. Four-hour infusions of LHRH in men with untreated Klinefelter's syndrome produced exaggerated responses for both LH and FSH. After 6 weeks of treatment with long-acting testosterone esters administered weekly (250 mg), the exaggerated responses for both FSH and LH were decreased. For LH, the decrease principally affected the initial phase of LH release, and the pattern obtained resembled more closely the biphasic pattern seen in normal men.", "contents": "Serum LH and FSH response in four-hour infusions of luteinizing hormone-releasing hormone in normal men, Sertoli cell only syndrome, and Klinefelter's syndrome. Constant intravenous infusion of 1 mug/min of luteinizing hormone-releasing hormone (LHRH) for 4 hours into normal men produced a biphasic pattern of LH secretion. The initial LH peak occurred 15-45 minutes after the commencement of the infusion, following which LH levels plateaued or declined, subsequently rising as the infusion continued. No biphasic pattern of secretion could be identified for FSH. During the infusion, no consistent testosterone rise could be identified despite significant elevation of LH levels. Similar infusions of LHRH in men with Sertoli cell only syndrome produced exaggerated responses in terms of both LH and FSH. In contrast, a single intravenous bolus of 100 mug/ LHRH produced an exaggerated response in only one of the 3 men studied, suggesting that the prolonged infusion may be a more discerning dynamic test. Four-hour infusions of LHRH in men with untreated Klinefelter's syndrome produced exaggerated responses for both LH and FSH. After 6 weeks of treatment with long-acting testosterone esters administered weekly (250 mg), the exaggerated responses for both FSH and LH were decreased. For LH, the decrease principally affected the initial phase of LH release, and the pattern obtained resembled more closely the biphasic pattern seen in normal men."}
{"id": "PMID:1102554", "title": "Comparison of short and long-term treatment with synthetic LH-releasing hormone and clomiphene citrate in male hypothalamic hypogonadism.", "content": "The effects of short and long-term treatment with clomiphene citrate and with synthetic LH-releasing hormone (LHRH) on gonadotropin release were studied in 5 male patients with hypothalamic hypogonadism. Neither short-term treatment with clomiphene citrate (50 mg or 200 mg daily for 4 days) nor long-term treatment (25 mg or 100 mg for 30-62 days) was effective in increasing serum LH and FSH in any patients. On the other hand, 5 cases showed slight or no rise in LH after administration of a single dose of 100 mug LH-releasing hormone (LHRH) and higher rises after administration of 400 mug. Four cases who were given 200 mug LHRH daily for 3 weeks showed an increasing response of serum LH and reached maximal LH levels at 2 weeks, followed by no further increase. In two of the four cases serum LH level reached normal adult male ranges. There was no increase of serum testosterone during LHRH treatment. These results suggest that long-term treatment with synthetic LHRH may be effective for gonadotropin restoration in some patients with hypothalamic hypogonadism in which long-term treatment of clomiphene citrate was shown to be ineffective.", "contents": "Comparison of short and long-term treatment with synthetic LH-releasing hormone and clomiphene citrate in male hypothalamic hypogonadism. The effects of short and long-term treatment with clomiphene citrate and with synthetic LH-releasing hormone (LHRH) on gonadotropin release were studied in 5 male patients with hypothalamic hypogonadism. Neither short-term treatment with clomiphene citrate (50 mg or 200 mg daily for 4 days) nor long-term treatment (25 mg or 100 mg for 30-62 days) was effective in increasing serum LH and FSH in any patients. On the other hand, 5 cases showed slight or no rise in LH after administration of a single dose of 100 mug LH-releasing hormone (LHRH) and higher rises after administration of 400 mug. Four cases who were given 200 mug LHRH daily for 3 weeks showed an increasing response of serum LH and reached maximal LH levels at 2 weeks, followed by no further increase. In two of the four cases serum LH level reached normal adult male ranges. There was no increase of serum testosterone during LHRH treatment. These results suggest that long-term treatment with synthetic LHRH may be effective for gonadotropin restoration in some patients with hypothalamic hypogonadism in which long-term treatment of clomiphene citrate was shown to be ineffective."}
{"id": "PMID:1102555", "title": "Plasma gonadotropins after administration of LH-releasing hormone in patients with renal or hepatic failure.", "content": "The pituitary gonadotropin response to the administration of synthetic LH-releasing hormone (LH RH) in 10 patients with renal failure and 5 patients with hepatic insufficiency was determined. The basal plasma LH and FSH levels were either normal or slightly elevated in both groups compared with the controls, but after the administration of LHRH, the peak levels (mean +/- SE mIU/ml) of LH (72.0 +/- 16.6) and FSH (18.7 +/- 4.4) in the patients with renal failure, and of LH (61.5 +/- 20.8) and FSH (13.2 +/- 2.8) in patients with hepatic insufficiency, were not significantly different from those in 11 normal controls (LH, 75.6 +/- 12.0; FSH, 12.4 +/- 2.9). However, significant elevations in plasma gonadotropin levels persisted in these patients during the second and third hours after the administration of LHRH. These studies do not resolve the question of whether the prolonged elevation in plasma LH and FSH levels after LHRH administration is due to enhanced secretion, to a diminished rate of metabolic clearance of LHRH, and/or to the gonadotropins, or both. Evidence from this and previous studies suggests that there may be defects in the secretory functions of both the hypothalamus and pituitary gland in some of these patients.", "contents": "Plasma gonadotropins after administration of LH-releasing hormone in patients with renal or hepatic failure. The pituitary gonadotropin response to the administration of synthetic LH-releasing hormone (LH RH) in 10 patients with renal failure and 5 patients with hepatic insufficiency was determined. The basal plasma LH and FSH levels were either normal or slightly elevated in both groups compared with the controls, but after the administration of LHRH, the peak levels (mean +/- SE mIU/ml) of LH (72.0 +/- 16.6) and FSH (18.7 +/- 4.4) in the patients with renal failure, and of LH (61.5 +/- 20.8) and FSH (13.2 +/- 2.8) in patients with hepatic insufficiency, were not significantly different from those in 11 normal controls (LH, 75.6 +/- 12.0; FSH, 12.4 +/- 2.9). However, significant elevations in plasma gonadotropin levels persisted in these patients during the second and third hours after the administration of LHRH. These studies do not resolve the question of whether the prolonged elevation in plasma LH and FSH levels after LHRH administration is due to enhanced secretion, to a diminished rate of metabolic clearance of LHRH, and/or to the gonadotropins, or both. Evidence from this and previous studies suggests that there may be defects in the secretory functions of both the hypothalamus and pituitary gland in some of these patients."}
{"id": "PMID:1102556", "title": "Serum LH and FSH responses to synthetic gonadotropin-releasing hormone in normal men.", "content": "The effects of dose and of age on the serum LH and FSH responses to synthetic gonadotropin-releasing hormone (GnRH) were determined in normal men. GnRH was given as in iv bolus in doses from 2 to 500 mug to each of nine normal men, 20-39 years old. The mean (+/- SE) maximum delta LH (maximum increment above the basal concentration) in response to each dose of GnRH was 2 mug, 7.2 +/- 1.7 mIU/ml; 10 mug, 15.7 +/- 3.4 mIU/ml; 50 mug, 37.7 +/- 9.6 mIU/ml; 250 mug, 47.8 +/- 11.2 mIU/ml; and 500 mug, 49.3 +/- 11.2 mIU/ml. The mean delta FSH in response to each dose was 2 mug, 0.5 +/- 0.2 mIU/ml; 10 mug, 0.8 +/- 0.2 mIU/ml; 50 mug, 1.2 +/- 0.3 mIU/ml; 250 mug, 2.0 +/- 0.3 mIU/ml; and 500 mug, 2.4 +/- 0.4 mIU/ml. Since both the LH and FSH responses to the 250 mug dose were significantly greater than those to smaller doses, but the responses to the 500 mug dose were not still larger, 250 mug was used as the standard test done for subsequent studies. The effect of age was tested by administering a 250 mug dose of GnRH as an iv bolus to 42 carefully selected normal men, 14 in each of three age groups, 20-39, 40-59, and 60-79 years old. Dialyzable serum testosterone levels decreased with increasing age, from 21.7 +/- 4.6 to 16.0 +/- 4.9 to 14.3 +/- 3.8 ng/dl (mean +/- SD), and the basal serum LH levels increased slightly, from 7.4 +/- 3.4 to 9.6 +/- 3.1 to 10.8 +/- 3.2 mIU/ml, suggesting that a slight degree of primary Leydig cell failure occurs with increasing age. There was no corresponding increase in mean maximum delta LH in response to GnRH with increasing age, despite the elevation in mean basal LH levels. The maximum delta LH/basal LH ratio, in fact, declined from 6.8 to 5.1 to 3.3 in the three age groups. The basal serum FSH levels did not change with increasing age, but the mean delta FSH values decreased from 3.0 +/- 0.5 to 2.9 +/- 1.1 +/- 0.3 mIU/ml. These data demonstrate decreased LH and FSH responses to synthetic GnRH, as well as some primary Leydig cell failure, in the male senescence.", "contents": "Serum LH and FSH responses to synthetic gonadotropin-releasing hormone in normal men. The effects of dose and of age on the serum LH and FSH responses to synthetic gonadotropin-releasing hormone (GnRH) were determined in normal men. GnRH was given as in iv bolus in doses from 2 to 500 mug to each of nine normal men, 20-39 years old. The mean (+/- SE) maximum delta LH (maximum increment above the basal concentration) in response to each dose of GnRH was 2 mug, 7.2 +/- 1.7 mIU/ml; 10 mug, 15.7 +/- 3.4 mIU/ml; 50 mug, 37.7 +/- 9.6 mIU/ml; 250 mug, 47.8 +/- 11.2 mIU/ml; and 500 mug, 49.3 +/- 11.2 mIU/ml. The mean delta FSH in response to each dose was 2 mug, 0.5 +/- 0.2 mIU/ml; 10 mug, 0.8 +/- 0.2 mIU/ml; 50 mug, 1.2 +/- 0.3 mIU/ml; 250 mug, 2.0 +/- 0.3 mIU/ml; and 500 mug, 2.4 +/- 0.4 mIU/ml. Since both the LH and FSH responses to the 250 mug dose were significantly greater than those to smaller doses, but the responses to the 500 mug dose were not still larger, 250 mug was used as the standard test done for subsequent studies. The effect of age was tested by administering a 250 mug dose of GnRH as an iv bolus to 42 carefully selected normal men, 14 in each of three age groups, 20-39, 40-59, and 60-79 years old. Dialyzable serum testosterone levels decreased with increasing age, from 21.7 +/- 4.6 to 16.0 +/- 4.9 to 14.3 +/- 3.8 ng/dl (mean +/- SD), and the basal serum LH levels increased slightly, from 7.4 +/- 3.4 to 9.6 +/- 3.1 to 10.8 +/- 3.2 mIU/ml, suggesting that a slight degree of primary Leydig cell failure occurs with increasing age. There was no corresponding increase in mean maximum delta LH in response to GnRH with increasing age, despite the elevation in mean basal LH levels. The maximum delta LH/basal LH ratio, in fact, declined from 6.8 to 5.1 to 3.3 in the three age groups. The basal serum FSH levels did not change with increasing age, but the mean delta FSH values decreased from 3.0 +/- 0.5 to 2.9 +/- 1.1 +/- 0.3 mIU/ml. These data demonstrate decreased LH and FSH responses to synthetic GnRH, as well as some primary Leydig cell failure, in the male senescence."}
{"id": "PMID:1102557", "title": "New technique for quantitative bacteriological sampling of wounds by velvet pads: clinical sampling trial.", "content": "A new surface sampling method, the velvet pad rinse technique, was compared with the velvet pad imprint method, in a randomized bacteriological sampling trial, using surgical wounds to evaluate quantitative results. The efficiency of bacterial recovery was increased 20-fold by the new technique, and the improvement was ascribed in particular to the introduction of a procedure of rinsing the velvet pad after sampling.", "contents": "New technique for quantitative bacteriological sampling of wounds by velvet pads: clinical sampling trial. A new surface sampling method, the velvet pad rinse technique, was compared with the velvet pad imprint method, in a randomized bacteriological sampling trial, using surgical wounds to evaluate quantitative results. The efficiency of bacterial recovery was increased 20-fold by the new technique, and the improvement was ascribed in particular to the introduction of a procedure of rinsing the velvet pad after sampling."}
{"id": "PMID:1102558", "title": "Reproducibility of the analytab (API 20E) system.", "content": "The reproducibility of the Analytab (API 20E) system for identification of Enterobacteriaceae was evaluated with 110 clinical isolates. Each isolate was identified by two technologists at different times. Genus-species identification was 97.3% reproducible; however, only 55.5% of the strains gave identical reactions in all 20 of the API 20E biochemical tests on repeat testing. Of those strains which varied, 56% possessed only one variable biochemical test. The reproducibility for each biochemical test was calculated and ranged from 89 to 100%. A subset of 20 of the most variable strains was tested further under conditions of varying incubation time (15 and 22 h) and inoculum concentration (10(7), 10(5), and 10(3) colony-forming units per ml), and by having four technologists interpret the test results. The reproducibility for each biochemical test for these 20 variable strains ranged from 86 to 99%. Less variation in interpretation by technologists was seen at an incubation time of 22 h and an inoculum concentration of 10(7) colony-forming units per ml. Consideration of the reproducibility for each biochemical test can aid in determining the probability that two isolates suspected of being the same strain, but with API profiles which differ by one or more biochemical test results, are in fact the same strain. Variables such as inoculum size, incubation time, technologist interpretation, and strip variability affect the API test results and should be standardized to minimize their effects.", "contents": "Reproducibility of the analytab (API 20E) system. The reproducibility of the Analytab (API 20E) system for identification of Enterobacteriaceae was evaluated with 110 clinical isolates. Each isolate was identified by two technologists at different times. Genus-species identification was 97.3% reproducible; however, only 55.5% of the strains gave identical reactions in all 20 of the API 20E biochemical tests on repeat testing. Of those strains which varied, 56% possessed only one variable biochemical test. The reproducibility for each biochemical test was calculated and ranged from 89 to 100%. A subset of 20 of the most variable strains was tested further under conditions of varying incubation time (15 and 22 h) and inoculum concentration (10(7), 10(5), and 10(3) colony-forming units per ml), and by having four technologists interpret the test results. The reproducibility for each biochemical test for these 20 variable strains ranged from 86 to 99%. Less variation in interpretation by technologists was seen at an incubation time of 22 h and an inoculum concentration of 10(7) colony-forming units per ml. Consideration of the reproducibility for each biochemical test can aid in determining the probability that two isolates suspected of being the same strain, but with API profiles which differ by one or more biochemical test results, are in fact the same strain. Variables such as inoculum size, incubation time, technologist interpretation, and strip variability affect the API test results and should be standardized to minimize their effects."}
{"id": "PMID:1102559", "title": "Identification of the elementary bodies of Chlamydia trachomatis in the electron microscope by an indirect immunoferritin technique.", "content": "An indirect immunoferritin (IIF) technique is described for recognizing the elementary bodies (EB) of Chlamydia trachomatis in unsectioned preparations. Both the EB of a genital strain of C. trachomatis grown in irradiated McCoy cells and EB in clinical specimens obtained from patients attending a venereal disease clinic were identified by the IIF test in the electron microscope. Cell culture-grown EB were detected by ferritin staining for up to 4 weeks after the organisms had lost their infectivity for tissue cultures. The IIF test was of comparable sensitivity to isolation methods in detecting chlamydiae in clinical specimens. Other possible applications of the IIF technique are discussed.", "contents": "Identification of the elementary bodies of Chlamydia trachomatis in the electron microscope by an indirect immunoferritin technique. An indirect immunoferritin (IIF) technique is described for recognizing the elementary bodies (EB) of Chlamydia trachomatis in unsectioned preparations. Both the EB of a genital strain of C. trachomatis grown in irradiated McCoy cells and EB in clinical specimens obtained from patients attending a venereal disease clinic were identified by the IIF test in the electron microscope. Cell culture-grown EB were detected by ferritin staining for up to 4 weeks after the organisms had lost their infectivity for tissue cultures. The IIF test was of comparable sensitivity to isolation methods in detecting chlamydiae in clinical specimens. Other possible applications of the IIF technique are discussed."}
{"id": "PMID:1102560", "title": "Indirect fluorescent antibody procedure for the rapid detection and identification of Bacteroides and Fusobacterium in clinical specimens.", "content": "An indirect fluorescent antibody (IFA) technique was evaluated as a procedure for rapid detection and identification of members of the Bacteroidaceae. Antisera were prepared against 31 members of this family, including species of Bacteroides and Fusobacterium commonly isolated from human infections. The antisera had demonstrated species and/or subspecies specificity. Thirty clinical specimens were studied. Of 13 specimens yielding Bacteroidaceae, for which antisera were available, 23 were presumptively diagnosed by IFA to contain subspecies of B. fragilis and/or Fusobacterium species. Of 17 specimens yielding negative culture results, two were positive by IFA on direct smear. Frequently the in vivo morphology of cells detected in direct smears by this procedure closely mimicked that of cellular debris, tissue cells, and leukocytes. Polyvalent antisera pools facilitated use of the IFA procedure as a practical tool for rapid diagnosis of infections involving the Bacteroidaceae.", "contents": "Indirect fluorescent antibody procedure for the rapid detection and identification of Bacteroides and Fusobacterium in clinical specimens. An indirect fluorescent antibody (IFA) technique was evaluated as a procedure for rapid detection and identification of members of the Bacteroidaceae. Antisera were prepared against 31 members of this family, including species of Bacteroides and Fusobacterium commonly isolated from human infections. The antisera had demonstrated species and/or subspecies specificity. Thirty clinical specimens were studied. Of 13 specimens yielding Bacteroidaceae, for which antisera were available, 23 were presumptively diagnosed by IFA to contain subspecies of B. fragilis and/or Fusobacterium species. Of 17 specimens yielding negative culture results, two were positive by IFA on direct smear. Frequently the in vivo morphology of cells detected in direct smears by this procedure closely mimicked that of cellular debris, tissue cells, and leukocytes. Polyvalent antisera pools facilitated use of the IFA procedure as a practical tool for rapid diagnosis of infections involving the Bacteroidaceae."}
{"id": "PMID:1102561", "title": "Germ tube and chlamydospore formation by Candida albicans on a new medium.", "content": "A new medium composed of \"cream of rice\" infusion, oxgall, Tween 80, and agar is described for the sequential development of germ tubes and chlamydospores by Candida albicans. The procedure used (Dalmau's technique) is an improvement over the fluid substrate procedures previously advocated for germ tube formation. That the same preparation is then used for chlamydospore production is of practical importance for the clinical mycology laboratory.", "contents": "Germ tube and chlamydospore formation by Candida albicans on a new medium. A new medium composed of \"cream of rice\" infusion, oxgall, Tween 80, and agar is described for the sequential development of germ tubes and chlamydospores by Candida albicans. The procedure used (Dalmau's technique) is an improvement over the fluid substrate procedures previously advocated for germ tube formation. That the same preparation is then used for chlamydospore production is of practical importance for the clinical mycology laboratory."}
{"id": "PMID:1102562", "title": "Unusual Enterobacteriaceae: lactose-positive Salmonella typhimurium which is endemic in S\u00e3o Paulo, Brazil.", "content": "Since 1971 a lactose-positive (lac+) Salmonella typhimurium variety Copenhagen has been endemic in the city of Sao Paulo. The strain is a strong lactose fermenter and resembles Escherichia coli on primary plating media and in triple sugar iron agar. Although most isolates of the strain have uniform properties, some have slightly different antigens, antibiograms, phage types, or fermentation patterns. Most isolates have come from stools of infants under 1 year of age and are probably hospital acquired; however, other isolates are probably community acquired. Eighteen other lac+ Salmonella isolated in the United States were also studied. Most of these strains resembled E. coli on primary plates and triple sugar iron agar; thus their identification would pose a problem for most clinical laboratories. A simple procedure for detecting lac+ Salmonella mixed with lac+ E. coli consists of touching 12 colonies in succession with a straight wire and then inoculating a peptone iron agar tube. H2S production is apparent from lac+ Salmonella even if 11 E. coli and one Salmonella colony are picked. If a positive peptone iron agar tube is observed, then individual colonies are tested to rule out other strong H2S producers. The true incidence of lac+ Salmonella is unknown because they are not isolated and identified in most laboratories.", "contents": "Unusual Enterobacteriaceae: lactose-positive Salmonella typhimurium which is endemic in S\u00e3o Paulo, Brazil. Since 1971 a lactose-positive (lac+) Salmonella typhimurium variety Copenhagen has been endemic in the city of Sao Paulo. The strain is a strong lactose fermenter and resembles Escherichia coli on primary plating media and in triple sugar iron agar. Although most isolates of the strain have uniform properties, some have slightly different antigens, antibiograms, phage types, or fermentation patterns. Most isolates have come from stools of infants under 1 year of age and are probably hospital acquired; however, other isolates are probably community acquired. Eighteen other lac+ Salmonella isolated in the United States were also studied. Most of these strains resembled E. coli on primary plates and triple sugar iron agar; thus their identification would pose a problem for most clinical laboratories. A simple procedure for detecting lac+ Salmonella mixed with lac+ E. coli consists of touching 12 colonies in succession with a straight wire and then inoculating a peptone iron agar tube. H2S production is apparent from lac+ Salmonella even if 11 E. coli and one Salmonella colony are picked. If a positive peptone iron agar tube is observed, then individual colonies are tested to rule out other strong H2S producers. The true incidence of lac+ Salmonella is unknown because they are not isolated and identified in most laboratories."}
{"id": "PMID:1102563", "title": "Evaluation of the Uni-Yeast-Tek kit for the identification of medically important yeasts.", "content": "The Uni-Yeast-Tek system, a commercially prepared kit and scheme for the rapid identification of medically important yeasts (Corning Medical), was evaluated in comparison with a conventional procedure in the identification of 623 yeasts. The system permitted the presumptive identification of 99.8% of 436 isolates representing 16 common species commonly isolated in the clinical laboratory. Correct biochemical and morphological analyses were obtained with 48 other species, but their specific identification required additional data.", "contents": "Evaluation of the Uni-Yeast-Tek kit for the identification of medically important yeasts. The Uni-Yeast-Tek system, a commercially prepared kit and scheme for the rapid identification of medically important yeasts (Corning Medical), was evaluated in comparison with a conventional procedure in the identification of 623 yeasts. The system permitted the presumptive identification of 99.8% of 436 isolates representing 16 common species commonly isolated in the clinical laboratory. Correct biochemical and morphological analyses were obtained with 48 other species, but their specific identification required additional data."}
{"id": "PMID:1102564", "title": "Formalinized bacterial \"antigens\" as a potential infection hazard.", "content": "It is widely thought that after enteric bacteric have been \"formalinized\" (treated with an equal volume of 0.6% formalin) for 1 h, the bacteria become \"antigens\" and are no longer viable. None of the 27 cultures of Salmonella and other Enterobacteriaceae were entirely killed within 1 h after formalin was added, but all 27 were reduced from 10(9) viable cells per ml to less than 10(2) per ml within 7 h. Thus, mouth pipetting of cultures formalinized for only 1 h is a possible infection hazard.", "contents": "Formalinized bacterial \"antigens\" as a potential infection hazard. It is widely thought that after enteric bacteric have been \"formalinized\" (treated with an equal volume of 0.6% formalin) for 1 h, the bacteria become \"antigens\" and are no longer viable. None of the 27 cultures of Salmonella and other Enterobacteriaceae were entirely killed within 1 h after formalin was added, but all 27 were reduced from 10(9) viable cells per ml to less than 10(2) per ml within 7 h. Thus, mouth pipetting of cultures formalinized for only 1 h is a possible infection hazard."}
{"id": "PMID:1102565", "title": "Immunochemical studies of the endocrine cells of the gastrointestinal tract. II An immunoperoxide technique for the localization of secretin containing cells in human duodenum.", "content": "Endocrine cells containing secretin have been identified in the epithelium lining human duodenum by direct and indirect immunoperoxidase techniques using immune sera raised against pur natural secretin. The techniques were applied to sections of carbodiimide-fixed tissue embedded in polyethylene glycol. Some sections, stained by a modified indirect technique, were processed for electron microscopy; secretin-containing granules were present by ultrastructural preservation was too poor to be of value. The potential advantages of a peroxidase technique over fluorescein-coniugated antisera are discussed.", "contents": "Immunochemical studies of the endocrine cells of the gastrointestinal tract. II An immunoperoxide technique for the localization of secretin containing cells in human duodenum. Endocrine cells containing secretin have been identified in the epithelium lining human duodenum by direct and indirect immunoperoxidase techniques using immune sera raised against pur natural secretin. The techniques were applied to sections of carbodiimide-fixed tissue embedded in polyethylene glycol. Some sections, stained by a modified indirect technique, were processed for electron microscopy; secretin-containing granules were present by ultrastructural preservation was too poor to be of value. The potential advantages of a peroxidase technique over fluorescein-coniugated antisera are discussed."}
{"id": "PMID:1102566", "title": "A clinical trial design avoiding undue placebo treatment.", "content": "A design is descirbed of a modified placebo-controlled double-blind clinical trial procedure. The design was evolved in attempt to overcome certain problems inherent in the conventional double-blind procedure, in particular the ethical problem of prolonged exposure of the patient to placebo treatment. The modified designs consist of two phases. In the first (open) phase, patients selected according to the normal protocol requirements are given the medication under study for a predetermined time. Patients responding favorably to such treatment then enter a second (double blind) phase during which is tested the null hypothesis that all the favorable effects observed during the open phase are placebo effects. Experience with this clinical trial design in the evaluation of an antianginal agent is briefly described. In this case, the null hypothesis was disproved, and a potent therapeutic action was evidenced.", "contents": "A clinical trial design avoiding undue placebo treatment. A design is descirbed of a modified placebo-controlled double-blind clinical trial procedure. The design was evolved in attempt to overcome certain problems inherent in the conventional double-blind procedure, in particular the ethical problem of prolonged exposure of the patient to placebo treatment. The modified designs consist of two phases. In the first (open) phase, patients selected according to the normal protocol requirements are given the medication under study for a predetermined time. Patients responding favorably to such treatment then enter a second (double blind) phase during which is tested the null hypothesis that all the favorable effects observed during the open phase are placebo effects. Experience with this clinical trial design in the evaluation of an antianginal agent is briefly described. In this case, the null hypothesis was disproved, and a potent therapeutic action was evidenced."}
{"id": "PMID:1102567", "title": "The effect of glucagon on airway resistance.", "content": "Nine subjects with obstructive airway disease took part in this double-blind study of the effect of glucagon in relieving bronchoconstriction compared with isoproterenol or isotonic saline. Drugs were administered via an in-line intermittent positive-pressure breathing unit. FEV1, MEF, PEF, and nitrogen washout data were evaluated. Ten minutes after administration of saline, mean FEV1 and MEF values were significantly reduced from baseline levels (P less than 0.05). Compared to saline, isoproterenol was followed by significantly increased FEV1 and MEF values (P less than 0.05) at both 10 and 30 minutes after medication, indicating reduction in bronchoconstriction. Although the 1-mg dose of glucagon was followed by a mean decrease in these parameters, this decrease was less than that seen after saline alone.", "contents": "The effect of glucagon on airway resistance. Nine subjects with obstructive airway disease took part in this double-blind study of the effect of glucagon in relieving bronchoconstriction compared with isoproterenol or isotonic saline. Drugs were administered via an in-line intermittent positive-pressure breathing unit. FEV1, MEF, PEF, and nitrogen washout data were evaluated. Ten minutes after administration of saline, mean FEV1 and MEF values were significantly reduced from baseline levels (P less than 0.05). Compared to saline, isoproterenol was followed by significantly increased FEV1 and MEF values (P less than 0.05) at both 10 and 30 minutes after medication, indicating reduction in bronchoconstriction. Although the 1-mg dose of glucagon was followed by a mean decrease in these parameters, this decrease was less than that seen after saline alone."}
{"id": "PMID:1102568", "title": "Cardiorespiratory response to a new isoquinoline derivative in critically III patients.", "content": "NC 7197, a new N-substituted tetrahydroisoquinoline derivative, was given in doses of 0.2 mg/kg body weight on 26 occasions to a series of 23 critically ill postoperative and posttraumatic patients who had been in moderate or severe degrees of shock. This agent was observed to improve pressure-flow and oxygen-transport variables, including increases in cardiac index, mean arterial pressure, central venous pressure, both left and right ventricular stroke work, central blood volume, systemic vascular resistance, oxygen availability, arteriovenous oxygen content difference, and oxygen consumption, and decreases in mean transit time and pulmonary vascular resistance. Previous studies on critically ill patients have suggested that these are the most commonly desired therapeutic actions for this type of patient. The agent has pronounced inotropic effect with minimal chronotropic effects, but with higher doses, chronotropic effects as well as alpha blocking effects may occur. The optimal effects may be obtained by adjusting the dose to an appropriate therapeutic range. It is concluded that, in the dose used, this agent produced both alpha and beta adrenergic actions in critically ill patients.", "contents": "Cardiorespiratory response to a new isoquinoline derivative in critically III patients. NC 7197, a new N-substituted tetrahydroisoquinoline derivative, was given in doses of 0.2 mg/kg body weight on 26 occasions to a series of 23 critically ill postoperative and posttraumatic patients who had been in moderate or severe degrees of shock. This agent was observed to improve pressure-flow and oxygen-transport variables, including increases in cardiac index, mean arterial pressure, central venous pressure, both left and right ventricular stroke work, central blood volume, systemic vascular resistance, oxygen availability, arteriovenous oxygen content difference, and oxygen consumption, and decreases in mean transit time and pulmonary vascular resistance. Previous studies on critically ill patients have suggested that these are the most commonly desired therapeutic actions for this type of patient. The agent has pronounced inotropic effect with minimal chronotropic effects, but with higher doses, chronotropic effects as well as alpha blocking effects may occur. The optimal effects may be obtained by adjusting the dose to an appropriate therapeutic range. It is concluded that, in the dose used, this agent produced both alpha and beta adrenergic actions in critically ill patients."}
{"id": "PMID:1102569", "title": "Metiapine: a double-blind comparison with chlorpromazine in acute schizophrenic patients.", "content": "Sixty newly admitted acute schizophrenic patients were randomly assigned to a double-blind trial of metiapine with a maximum dose of 450 mg per day versus a maximum dose of 450 mg per day versus a maximum daily dose of 900 mg chlorpromazine per day. At the conclusion of the study, 21 patients in each group showed marked to moderate improvement. There were significantly more marked improvers in the metiapine group then the chlorpromazine group on the Physician's Posttreatment Global Impression. Evaluation by analysis of covariance of the Brief Psychiatric Rating Scale showed a significant difference between treatment groups favoring chlorpromazine on the item of blunted affect. The spectrum of side effects was similar in the two groups, except for six patients treated with metiapine who displayed tachycardia on the EKG. This pulse elevation was reflected in the group data and is probably dose related. In conclusion, both drugs appeared to be equally efficacious in the treatment of newly admitted acute schizophrenic patients.", "contents": "Metiapine: a double-blind comparison with chlorpromazine in acute schizophrenic patients. Sixty newly admitted acute schizophrenic patients were randomly assigned to a double-blind trial of metiapine with a maximum dose of 450 mg per day versus a maximum dose of 450 mg per day versus a maximum daily dose of 900 mg chlorpromazine per day. At the conclusion of the study, 21 patients in each group showed marked to moderate improvement. There were significantly more marked improvers in the metiapine group then the chlorpromazine group on the Physician's Posttreatment Global Impression. Evaluation by analysis of covariance of the Brief Psychiatric Rating Scale showed a significant difference between treatment groups favoring chlorpromazine on the item of blunted affect. The spectrum of side effects was similar in the two groups, except for six patients treated with metiapine who displayed tachycardia on the EKG. This pulse elevation was reflected in the group data and is probably dose related. In conclusion, both drugs appeared to be equally efficacious in the treatment of newly admitted acute schizophrenic patients."}
{"id": "PMID:1102570", "title": "Correlation of serum creatinine concentration and amikacin half-life.", "content": "The half-life of amikacin in serum has been measured following the administration of a single intravenous injection of 7 mg/kg in nine adult patients with varying degrees of renal function impairment and in four patients with normal renal function. A linear relationship between the half-life and the serum concentration has been found, suggesting that half-life of amikacin in serum can be calculated by multiplying the serum creatinine (in mg/100 ml) by 3.", "contents": "Correlation of serum creatinine concentration and amikacin half-life. The half-life of amikacin in serum has been measured following the administration of a single intravenous injection of 7 mg/kg in nine adult patients with varying degrees of renal function impairment and in four patients with normal renal function. A linear relationship between the half-life and the serum concentration has been found, suggesting that half-life of amikacin in serum can be calculated by multiplying the serum creatinine (in mg/100 ml) by 3."}
{"id": "PMID:1102573", "title": "Variability in mineral and trace element content of dairy cattle feeds.", "content": "Trace element content of feeds is extremely variable compared to total digestible nutrients or protein. Differences are wide between low and high values for many trace elements within a given type of feed. Coefficients of variation for forages range from 42 to 100%. Summary data are given for both major and trace elements. Problems in supplementation are discussed. Amounts for some elements are skewed with relatively few samples around the mean and over 60% below the mean. Suggested mineral allowances for dairy cows are listed. Under current supplementation, zinc is the trace element most likely to be limiting and manganese the least likely. Iron, copper, cobalt, and iodine may be limiting in some cases. Needs of research and applied nutrition are presented.", "contents": "Variability in mineral and trace element content of dairy cattle feeds. Trace element content of feeds is extremely variable compared to total digestible nutrients or protein. Differences are wide between low and high values for many trace elements within a given type of feed. Coefficients of variation for forages range from 42 to 100%. Summary data are given for both major and trace elements. Problems in supplementation are discussed. Amounts for some elements are skewed with relatively few samples around the mean and over 60% below the mean. Suggested mineral allowances for dairy cows are listed. Under current supplementation, zinc is the trace element most likely to be limiting and manganese the least likely. Iron, copper, cobalt, and iodine may be limiting in some cases. Needs of research and applied nutrition are presented."}
{"id": "PMID:1102574", "title": "New concepts and developments in metabolism and homeostasis of inorganic elements in dairy cattle. A review.", "content": "Major routes of homeostatic control animals use to adapt to widely variable intakes of minerals and the necessity of keeping tissue content of functional forms relatively constant are changes in: (1) percentage absorbed, (2) urinary excretion, (3) tissue deposition in harmless or mobilizable reserve forms, (4) secretion into milk, and (5) endogenous excretion via feces. The importance of each varies greatly among elements. Changes in absorption are of major importance with calcium, iron, zinc, and manganese but not with cadmium or iodine. Urine is a major control route for magnesium, fluorine, selenium, iodine, sodium, chlorine, and potassium. Milk iodine varies almost directly with intake. Tissue deposition and withdrawal are key routes for iron, copper, fluorine, and calcium. Endogenous (metabolic fecal) excretion is crucial in manganese homeostasis. Biochemical details of most homeostatic control mechanisms have not been established. Apparently changes in body tissues control percentage absorption of several elements, including zinc, at the intestine which is the control site. Other major factors which may affect metabolism of several elements include chemical form, whether the elements include chemical form, whether the element is absorbed or injected, and genetic differences among animals. Several common pitfalls and special research problems are discussed. New research indicates that silicon, vanadium, nickel, tin, and fluorine are probably essential.", "contents": "New concepts and developments in metabolism and homeostasis of inorganic elements in dairy cattle. A review. Major routes of homeostatic control animals use to adapt to widely variable intakes of minerals and the necessity of keeping tissue content of functional forms relatively constant are changes in: (1) percentage absorbed, (2) urinary excretion, (3) tissue deposition in harmless or mobilizable reserve forms, (4) secretion into milk, and (5) endogenous excretion via feces. The importance of each varies greatly among elements. Changes in absorption are of major importance with calcium, iron, zinc, and manganese but not with cadmium or iodine. Urine is a major control route for magnesium, fluorine, selenium, iodine, sodium, chlorine, and potassium. Milk iodine varies almost directly with intake. Tissue deposition and withdrawal are key routes for iron, copper, fluorine, and calcium. Endogenous (metabolic fecal) excretion is crucial in manganese homeostasis. Biochemical details of most homeostatic control mechanisms have not been established. Apparently changes in body tissues control percentage absorption of several elements, including zinc, at the intestine which is the control site. Other major factors which may affect metabolism of several elements include chemical form, whether the elements include chemical form, whether the element is absorbed or injected, and genetic differences among animals. Several common pitfalls and special research problems are discussed. New research indicates that silicon, vanadium, nickel, tin, and fluorine are probably essential."}
{"id": "PMID:1102575", "title": "Selenium in ruminant nutrition: a review.", "content": "The early interest in selenium related primarily to its toxicity, but since 1957 the element has been recognized as a dietary essential. The dietary requirement for selenium by most species is about .1 ppm. Deficiencies of selenium in cattle and sheep have been confirmed under natural grazing conditions in many countries of the world. Overt signs of inadequacy such as white muscle disease (nutritional muscular dystrophy) occur primarily in young calves or lambs born to selenium deficient dams. Infertility has increased in ewes grazing pastures low in selenium. In general, signs of deficiency have not occurred in older animals such as finishing beef cattle and lactating dairy cows. Subclinical deficiencies of selenium are not determined easily, however, and thus an inadequacy of the element may be limiting maximum animal performance under certain circumstances of drylot feeding. The current nutritional status of ruminant animals in many geographical areas and involving various feeding programs with this element has not been established. The recent widespread deficiency problems with nonruminants suggest that such an assessment should be made. Concentration of selenium in tissue, particularly in the liver, has been used in establishing selenium status of the animal. With lambs glutathione peroxidase activity in certain tissues may be a more accurate indicator of selenium adequacy than is selenium content of the tissue. Supplemental sodium selenite and sodium selenate by either oral administration or parenteral injection have prevented clinical signs of selenium deficiency and animal losses in both ruminant and nonruminant animals. Heavy pellets containing elemental selenium for placement in the rumen have proved effective. In general, organic forms of selenium are absorbed more readily by animals than are inorganic compounds. The dietary requirements for selenium and its metabolism are influenced by many nutrient interrelationships, including its interactions with sulfur, lipids, vitamin E, proteins, amino acids, and several microelements. The Food and Drug Administration gave approval in 1974 for the oral administration of supplemental selenium as either sodium selenite or sodium selenate to certain classes of swine and poultry. Similar approval in the United States for ruminants will require additional information, particularly with regard to the influence of dietary intake on concentrations of selenium in tissue and milk in beef and dairy animals.", "contents": "Selenium in ruminant nutrition: a review. The early interest in selenium related primarily to its toxicity, but since 1957 the element has been recognized as a dietary essential. The dietary requirement for selenium by most species is about .1 ppm. Deficiencies of selenium in cattle and sheep have been confirmed under natural grazing conditions in many countries of the world. Overt signs of inadequacy such as white muscle disease (nutritional muscular dystrophy) occur primarily in young calves or lambs born to selenium deficient dams. Infertility has increased in ewes grazing pastures low in selenium. In general, signs of deficiency have not occurred in older animals such as finishing beef cattle and lactating dairy cows. Subclinical deficiencies of selenium are not determined easily, however, and thus an inadequacy of the element may be limiting maximum animal performance under certain circumstances of drylot feeding. The current nutritional status of ruminant animals in many geographical areas and involving various feeding programs with this element has not been established. The recent widespread deficiency problems with nonruminants suggest that such an assessment should be made. Concentration of selenium in tissue, particularly in the liver, has been used in establishing selenium status of the animal. With lambs glutathione peroxidase activity in certain tissues may be a more accurate indicator of selenium adequacy than is selenium content of the tissue. Supplemental sodium selenite and sodium selenate by either oral administration or parenteral injection have prevented clinical signs of selenium deficiency and animal losses in both ruminant and nonruminant animals. Heavy pellets containing elemental selenium for placement in the rumen have proved effective. In general, organic forms of selenium are absorbed more readily by animals than are inorganic compounds. The dietary requirements for selenium and its metabolism are influenced by many nutrient interrelationships, including its interactions with sulfur, lipids, vitamin E, proteins, amino acids, and several microelements. The Food and Drug Administration gave approval in 1974 for the oral administration of supplemental selenium as either sodium selenite or sodium selenate to certain classes of swine and poultry. Similar approval in the United States for ruminants will require additional information, particularly with regard to the influence of dietary intake on concentrations of selenium in tissue and milk in beef and dairy animals."}
{"id": "PMID:1102583", "title": "A historical review of complete denture impression materials.", "content": "The history of complete denture impression procefures has been influenced largely by the development of impression materials from which new ideas and techniques arose. Apparently, some procedures have been devised solely for the promotion of certain impression materials. For the most part, however, impression materials have been developed to accomplish specific goals and, at the time at least, were considered desirable for the purpose.", "contents": "A historical review of complete denture impression materials. The history of complete denture impression procefures has been influenced largely by the development of impression materials from which new ideas and techniques arose. Apparently, some procedures have been devised solely for the promotion of certain impression materials. For the most part, however, impression materials have been developed to accomplish specific goals and, at the time at least, were considered desirable for the purpose."}
{"id": "PMID:1102584", "title": "Dental consideration for the child with cleft palate.", "content": "The dentist's role in the care of the child with cleft palate involves his delivery of the examination, restorative and preventive procedures, and prosthetic, orthodontic, and orthopedic therapy. The practitioner is urged to enthusiastically and intelligently render treatment to these children to the full extent of his capabilities. It also must be remembered that because of the nature of the defect, children with clefts frequently are under demanding therapy from many specialists over their years of growth and development. We must not neglect our obligation to these individuals, but priorities must be taken into consideration for the overall best interest of the patient.", "contents": "Dental consideration for the child with cleft palate. The dentist's role in the care of the child with cleft palate involves his delivery of the examination, restorative and preventive procedures, and prosthetic, orthodontic, and orthopedic therapy. The practitioner is urged to enthusiastically and intelligently render treatment to these children to the full extent of his capabilities. It also must be remembered that because of the nature of the defect, children with clefts frequently are under demanding therapy from many specialists over their years of growth and development. We must not neglect our obligation to these individuals, but priorities must be taken into consideration for the overall best interest of the patient."}
{"id": "PMID:1102589", "title": "Experimental production of granulomatous pneumonitis. Comparison of immunological and morphological sequelae with particulate and soluble antigens administered via the respiratory route.", "content": "Rabbits were sensitized with either a soluble protein antigen (BSA) or a particulate thermophilic actinomycete antigen (Micropolyspora faeni) via the respiratory route, followed by monitoring of sequential morphologic changes and the humoral plus cellular immunologic response. Primary respiratory tract sensitization with BSA resulted in a humoral anti-BSA response, Arthus and delayed skin reactivity, and in some cases specific antigen-induced alveolar macrophage migration inhibition, all in the absence of pulmonary lesions. Lesions characterized by mild multifocal perivascular mononuclear cell infiltrates in the lungs developed only after secondary BSA aerosol challenge. In contrast to these findings, \"primary\" respiratory tract sensitization with M. faeni particulate antigen in saline solution resulted in the gradual development of extensive and progressive pulmonary interstitial and alveolar mononuclear cell infiltrates. These lesions were uniformly associated with specific serum precipitating antibody and delayed skin reactivity. Alveolar macrophage migration was significantly inhibited by Micropolyspora faeni in virtually of these animals. These results, while not excluding a primary irritant effect or Type II or III alergic tissue injury, suggest a role for delayed (cell-mediated) hypersensitivity in the pathogenesis of particulate actinomycete-induced pulmonary lesions. They also indicate that primary immunization with soluble purified protein antigens via the respiratory route can lead to systemic humoral and cell-mediated immunity without production of pulmonary lesions.", "contents": "Experimental production of granulomatous pneumonitis. Comparison of immunological and morphological sequelae with particulate and soluble antigens administered via the respiratory route. Rabbits were sensitized with either a soluble protein antigen (BSA) or a particulate thermophilic actinomycete antigen (Micropolyspora faeni) via the respiratory route, followed by monitoring of sequential morphologic changes and the humoral plus cellular immunologic response. Primary respiratory tract sensitization with BSA resulted in a humoral anti-BSA response, Arthus and delayed skin reactivity, and in some cases specific antigen-induced alveolar macrophage migration inhibition, all in the absence of pulmonary lesions. Lesions characterized by mild multifocal perivascular mononuclear cell infiltrates in the lungs developed only after secondary BSA aerosol challenge. In contrast to these findings, \"primary\" respiratory tract sensitization with M. faeni particulate antigen in saline solution resulted in the gradual development of extensive and progressive pulmonary interstitial and alveolar mononuclear cell infiltrates. These lesions were uniformly associated with specific serum precipitating antibody and delayed skin reactivity. Alveolar macrophage migration was significantly inhibited by Micropolyspora faeni in virtually of these animals. These results, while not excluding a primary irritant effect or Type II or III alergic tissue injury, suggest a role for delayed (cell-mediated) hypersensitivity in the pathogenesis of particulate actinomycete-induced pulmonary lesions. They also indicate that primary immunization with soluble purified protein antigens via the respiratory route can lead to systemic humoral and cell-mediated immunity without production of pulmonary lesions."}
{"id": "PMID:1102593", "title": "Studies on immune competence in the rat: changes with age, sex, and strain.", "content": "Reticuloendothelial capacity (determined by colloidal carbon clearance) and cell-mediated immunity (determined by xenograft skin grafts and macrophage migration inhibition in vitro) were studied as a function of age for the first time in male and female Sprague-Dawley rats. The results of all three tests in the female rat indicated that maximal immune competence was reached at the onset of puberty (4 weeks) and, depending on the immune response, immune competence declined significantly with age (4 to 96 weeks). In the male Sprague-Dawley, a similar pattern was found in two out of the three tests (colloidal carbon clearance, xenograft skin grafts). Macrophage migration inhibition in the male was unaffected by age (4 to 40 weeks). Cell-mediated immunity (xenograft skin grafts) was also studied in male and female rats of another strain (Long Evans) as a function of age. Maximal competence occurred at the onset of puberty and declined thereafter.", "contents": "Studies on immune competence in the rat: changes with age, sex, and strain. Reticuloendothelial capacity (determined by colloidal carbon clearance) and cell-mediated immunity (determined by xenograft skin grafts and macrophage migration inhibition in vitro) were studied as a function of age for the first time in male and female Sprague-Dawley rats. The results of all three tests in the female rat indicated that maximal immune competence was reached at the onset of puberty (4 weeks) and, depending on the immune response, immune competence declined significantly with age (4 to 96 weeks). In the male Sprague-Dawley, a similar pattern was found in two out of the three tests (colloidal carbon clearance, xenograft skin grafts). Macrophage migration inhibition in the male was unaffected by age (4 to 40 weeks). Cell-mediated immunity (xenograft skin grafts) was also studied in male and female rats of another strain (Long Evans) as a function of age. Maximal competence occurred at the onset of puberty and declined thereafter."}
{"id": "PMID:1102596", "title": "Effect of Cs137 on immunological reactivity (review of literature).", "content": "An important role of Cs137 as a new ecological factor was shown by analyzing the works of 31 authors. This radioisotope may at present be deceted in the organisms of all the inhabitants of this planet. The migration of Cs137 along the chain lichen-deer-man leads to its accumulation in the organism of human subjects living in the Extreme North and utilizing venison in their diet. Although the high sensitivity of immunological reactions to various unfavourable environmental factors is well known, the materials on the effect of incorporated Cs137 on immunity are scanty, Experiments on animals have shown changes in factors of nonspecific immunity (phagocytic reaction of blood neutrophils, bactericidal activity, lysozyme and complement titres of blood serum) and specific immunity (formation of antiviral antibodies). The blood of animals injured by the isotope displays complete and incomplete autoantibodies. A dependance between the immunobiological changes and the dose absorbed by the organism was demonstrated. The entry of CS137 into the organism of the inhabitants of the Extreme North who use venison in their diet did not, with the absorbed dose equalling up to 50 Mrem per year, lead to changes in their immunological reactivity.", "contents": "Effect of Cs137 on immunological reactivity (review of literature). An important role of Cs137 as a new ecological factor was shown by analyzing the works of 31 authors. This radioisotope may at present be deceted in the organisms of all the inhabitants of this planet. The migration of Cs137 along the chain lichen-deer-man leads to its accumulation in the organism of human subjects living in the Extreme North and utilizing venison in their diet. Although the high sensitivity of immunological reactions to various unfavourable environmental factors is well known, the materials on the effect of incorporated Cs137 on immunity are scanty, Experiments on animals have shown changes in factors of nonspecific immunity (phagocytic reaction of blood neutrophils, bactericidal activity, lysozyme and complement titres of blood serum) and specific immunity (formation of antiviral antibodies). The blood of animals injured by the isotope displays complete and incomplete autoantibodies. A dependance between the immunobiological changes and the dose absorbed by the organism was demonstrated. The entry of CS137 into the organism of the inhabitants of the Extreme North who use venison in their diet did not, with the absorbed dose equalling up to 50 Mrem per year, lead to changes in their immunological reactivity."}
{"id": "PMID:1102597", "title": "Study of rickettsioses in Slovakia. II. Infestation of fleas and mites in mole nests in some localities in Central Slovakia with C. burneti and Rickettsiae belonging to the spotted fever (SF) group.", "content": "Fleas and mites obtained from 115 mole nests in Central Slovakia from an area where Q fever and rickettsiosis due to rickettsie of the SF occur, from the border of this area and apart from it, were tested for infestation with rickettsiae by the method of isolation of rickettsiae on chick embryo yolk sacs, by the method of detection of rickettsiae on smears from the arthropods using immunofluorescence, by the method of exposure of albino mice and Apodemus flavicollis to mole nests and by the method of inoculation of suspension from the arthropods to albino mice. In the latter two methods, infestation of the arthropods was assessed according to the formation of antibodies in the mice. It has been found that fleas and mites in all three areas under test are infected with C. burneti and with rickettsia of the SF group. C. burneti was currently detected in the focus of rickettsioses and in the localities situated in its borders while only one case was observed apart from the focus. Rickettsiae of the SF group were found in fleas and mites in all areas tested showing no greater percentual differences. The method of detection of rickettsia in smears from the arthropods using the immunofluorescence technique and antibody response in mice inoculated with infected arthropods was found to be the most effective in these studies. On the basis of the obtained results the authors believe that mites and fleas living in mole nests participate in the circulation of both species of rickettsiae in nature in the transfer of rickettsiae among small mammals. The question whether these arthropods are a reservoir, a biological or a mechanical vector, still remains open.", "contents": "Study of rickettsioses in Slovakia. II. Infestation of fleas and mites in mole nests in some localities in Central Slovakia with C. burneti and Rickettsiae belonging to the spotted fever (SF) group. Fleas and mites obtained from 115 mole nests in Central Slovakia from an area where Q fever and rickettsiosis due to rickettsie of the SF occur, from the border of this area and apart from it, were tested for infestation with rickettsiae by the method of isolation of rickettsiae on chick embryo yolk sacs, by the method of detection of rickettsiae on smears from the arthropods using immunofluorescence, by the method of exposure of albino mice and Apodemus flavicollis to mole nests and by the method of inoculation of suspension from the arthropods to albino mice. In the latter two methods, infestation of the arthropods was assessed according to the formation of antibodies in the mice. It has been found that fleas and mites in all three areas under test are infected with C. burneti and with rickettsia of the SF group. C. burneti was currently detected in the focus of rickettsioses and in the localities situated in its borders while only one case was observed apart from the focus. Rickettsiae of the SF group were found in fleas and mites in all areas tested showing no greater percentual differences. The method of detection of rickettsia in smears from the arthropods using the immunofluorescence technique and antibody response in mice inoculated with infected arthropods was found to be the most effective in these studies. On the basis of the obtained results the authors believe that mites and fleas living in mole nests participate in the circulation of both species of rickettsiae in nature in the transfer of rickettsiae among small mammals. The question whether these arthropods are a reservoir, a biological or a mechanical vector, still remains open."}
{"id": "PMID:1102598", "title": "Some immunochemical and chemical aspects of S and R Shigella dysenteriae 1 endotoxins.", "content": "Endotoxins of S and R forms of Shigella dysenteriae 1 were prepared by NaCl-Na citrate extraction, purified by gel chromatography on Sephadex G 200 and on Sepharose 4B and subjected to immunochemical and chemical analysis. The toxins contained 25--30% of lipids, 40--50% of carbohydrates and 14--24% of protein. The lipid and protein moieties of the lipopolysaccharide-protein complexes exhibited no significant difference, whereas the sugar moieties differed markedly (both qualitatively and quantitatively), in relation to the growth form of the culture. The lipid moiety, which consists at least of 22 fatty acids, has the greatest relative content (approx. 50%) of behenic acid, 22:0, and palmitic acid, 16:0 (approx. 11%). In the protein moiety, at least 16 amino acids were determined; these amino acids were identical in both endotoxin types, but their total content was higher in the R form, giving an R:S ratio of 1.7 +/- 0.2. The sugar moiety consists of galactose, glucosamine and either rhamnose (in S endotoxin) or aldoheptose (in R endotoxin). The difference of the chemical composition of the sugar moiety is believed to account for the diametric difference in the immunochemical character, in particular the different behaviour in the electric field, of both endotoxin types. The average content of 3-deoxy-D-manno-2-octulosonic acid was determined as 0.5% for both S and R endotoxin. Trace amounts of O-phosphorylethanolamine were found. Individual aspects of the chemical and immunochemical analysis are discussed in detail.", "contents": "Some immunochemical and chemical aspects of S and R Shigella dysenteriae 1 endotoxins. Endotoxins of S and R forms of Shigella dysenteriae 1 were prepared by NaCl-Na citrate extraction, purified by gel chromatography on Sephadex G 200 and on Sepharose 4B and subjected to immunochemical and chemical analysis. The toxins contained 25--30% of lipids, 40--50% of carbohydrates and 14--24% of protein. The lipid and protein moieties of the lipopolysaccharide-protein complexes exhibited no significant difference, whereas the sugar moieties differed markedly (both qualitatively and quantitatively), in relation to the growth form of the culture. The lipid moiety, which consists at least of 22 fatty acids, has the greatest relative content (approx. 50%) of behenic acid, 22:0, and palmitic acid, 16:0 (approx. 11%). In the protein moiety, at least 16 amino acids were determined; these amino acids were identical in both endotoxin types, but their total content was higher in the R form, giving an R:S ratio of 1.7 +/- 0.2. The sugar moiety consists of galactose, glucosamine and either rhamnose (in S endotoxin) or aldoheptose (in R endotoxin). The difference of the chemical composition of the sugar moiety is believed to account for the diametric difference in the immunochemical character, in particular the different behaviour in the electric field, of both endotoxin types. The average content of 3-deoxy-D-manno-2-octulosonic acid was determined as 0.5% for both S and R endotoxin. Trace amounts of O-phosphorylethanolamine were found. Individual aspects of the chemical and immunochemical analysis are discussed in detail."}
{"id": "PMID:1102599", "title": "The effects of chemotherapeutics on the species streptococcus in vitro.", "content": "The authors studied the sensitivity of some rather recent products--flucloxacillin, doxycyclin, clindamycin and cotrimoxazol--in correlation with classical penicillin. If therapy by means of classical penicillins is unsuitable, clindamycin and the semi-synthetic penicillin flucloxacillin appear to be a further alternative and the most suitable chemotherapeutic in streptococcal infections.", "contents": "The effects of chemotherapeutics on the species streptococcus in vitro. The authors studied the sensitivity of some rather recent products--flucloxacillin, doxycyclin, clindamycin and cotrimoxazol--in correlation with classical penicillin. If therapy by means of classical penicillins is unsuitable, clindamycin and the semi-synthetic penicillin flucloxacillin appear to be a further alternative and the most suitable chemotherapeutic in streptococcal infections."}
{"id": "PMID:1102600", "title": "Immunologic functions of isolated human lymphocyte subpopulations. VI. Further characterization of the surface Ig negative, E rosette negative (null cell) subset.", "content": "Sephadex G-200 anti-human Fab column chromatography and rosette depletion techniques were used to isolate three distinct subpopulations of human lymphocytes: 1) T cells which are surface Ig negative and E rosette positive, 2) B cells which are surface Ig positive and E rosette negative, and 3) a \"Null\" cell population which is both surface Ig negative and E rosette negative. All populations were analyzed for their capacity to develop surface Ig and synthesize Ig in vitro. Greater than 50% of cells in the Null cell population developed surface Ig by day 3 of cell culture. Furthermore, in vitro, the Ig content of the Null cell population, as well as their capacity to secrete Ig in culture, becomes comparable to that produced by B cells. In contrast, cultured T cells neither develop surface Ig nor secrete Ig in culture. These data strongly support the idea that the Null population contains a subset of Ig-producing B cells.", "contents": "Immunologic functions of isolated human lymphocyte subpopulations. VI. Further characterization of the surface Ig negative, E rosette negative (null cell) subset. Sephadex G-200 anti-human Fab column chromatography and rosette depletion techniques were used to isolate three distinct subpopulations of human lymphocytes: 1) T cells which are surface Ig negative and E rosette positive, 2) B cells which are surface Ig positive and E rosette negative, and 3) a \"Null\" cell population which is both surface Ig negative and E rosette negative. All populations were analyzed for their capacity to develop surface Ig and synthesize Ig in vitro. Greater than 50% of cells in the Null cell population developed surface Ig by day 3 of cell culture. Furthermore, in vitro, the Ig content of the Null cell population, as well as their capacity to secrete Ig in culture, becomes comparable to that produced by B cells. In contrast, cultured T cells neither develop surface Ig nor secrete Ig in culture. These data strongly support the idea that the Null population contains a subset of Ig-producing B cells."}
{"id": "PMID:1102601", "title": "Ly antigens: markers of T cell function on mouse spleen cells.", "content": "By the use of Ly antisera and complement it is possible to remove subpopulations of mouse T cells from spleen cell suspension. It is shown that these Ly antisera are specific for the Ly alleles carried by the cells. Helper cells from primed or unprimed mice and cells which respond to Con A express Ly-1 antigen but little or no Ly-2 antigen (Ly-1+ phenotype) whereas cells which respond to PHA express both Ly-1 and Ly-2 antigens (Ly-1+2+ phenotype).", "contents": "Ly antigens: markers of T cell function on mouse spleen cells. By the use of Ly antisera and complement it is possible to remove subpopulations of mouse T cells from spleen cell suspension. It is shown that these Ly antisera are specific for the Ly alleles carried by the cells. Helper cells from primed or unprimed mice and cells which respond to Con A express Ly-1 antigen but little or no Ly-2 antigen (Ly-1+ phenotype) whereas cells which respond to PHA express both Ly-1 and Ly-2 antigens (Ly-1+2+ phenotype)."}
{"id": "PMID:1102602", "title": "Oxisuran and immune reactions: mediation of oxisuran action by the adrenal glands.", "content": "Oxisuran was tested in a TAR system and for the DH on SRBC. Besides immunosuppressive properties in the TAR and DH also mentioned by other authors, stimulation was found after prolonged administration. No effect of oxisuran on the humoral response was observed. The action of oxisuran was suggested to be indirect, effected by stimulation of the PAG system. Adrenalectomy of mice made animals non-liable for oxisuran action measured in the TAR assay. Oxisuran caused an increase of the corticosterone plasma level with a peak after four daily injections. This was paralleled by a peak in adrenal gland weight and a minimum in thymus size. The reduction in these effects after five injections (tachyphylaxis) suggest that a feedback mechanism is involved. Comparison of thymus weights after oxisuran and corticosterone administration indicates that a daily dose of 100 mg oxisuran/kg corresponds with 1.5 mg corticosterone/kg. The effects of oxisuran in the TAR and on the DH and the feedback phenomenon could be imitated with 1.5 mg corticosterone/kg. The mononuclear phagocyte has been proposed as the main target cell of oxisuran action, mediated by the PAG system.", "contents": "Oxisuran and immune reactions: mediation of oxisuran action by the adrenal glands. Oxisuran was tested in a TAR system and for the DH on SRBC. Besides immunosuppressive properties in the TAR and DH also mentioned by other authors, stimulation was found after prolonged administration. No effect of oxisuran on the humoral response was observed. The action of oxisuran was suggested to be indirect, effected by stimulation of the PAG system. Adrenalectomy of mice made animals non-liable for oxisuran action measured in the TAR assay. Oxisuran caused an increase of the corticosterone plasma level with a peak after four daily injections. This was paralleled by a peak in adrenal gland weight and a minimum in thymus size. The reduction in these effects after five injections (tachyphylaxis) suggest that a feedback mechanism is involved. Comparison of thymus weights after oxisuran and corticosterone administration indicates that a daily dose of 100 mg oxisuran/kg corresponds with 1.5 mg corticosterone/kg. The effects of oxisuran in the TAR and on the DH and the feedback phenomenon could be imitated with 1.5 mg corticosterone/kg. The mononuclear phagocyte has been proposed as the main target cell of oxisuran action, mediated by the PAG system."}
{"id": "PMID:1102603", "title": "Histocompatibility and T-B cell cooperation in mouse radiation chimeras.", "content": "Cooperative interaction between histocompatible as well as histoincompatible T and B cells in vivo was studied with B cell-bearing syngeneic radiation chimeras receiving syngeneic, semiallogeneic, or allogeneic A and T cells in various combinations. The results indicated the following: i) Histoincompatible T and B cells normally did not cooperate successfully for generation of antibody-forming cells. ii) Semiallogeneic (C3BF1) T cells cooperated successfully with parent-type (C3H) B cells developed in parent-type syngeneic chimeras (C3H/C3H), whereas parent-type (C3H as well as C57BL) T cells failed to cooperate with F1 (BC3F1) B cells developed in F1 syngeneic chimeras (BC3F1/BC3F1). iii) T cells obtained from C57BL/C3H or C57BL/C3BF1 chimeras, which were most likely donor (C57BL)- derived, cooperated successfully with C3H-derived B cells developed in C3H/C3H chimeras. iv) Evidence was obtained suggesting that stimulation of antibody response by allogeneic effect in the absence of syngeneic or semisyngeneic helper T cells did not take place in this experimental system. v) With the use of congenic resistant strains, it was shown that alloantigens controlled by H-2 loci, in particular by the K-I region of this gene complex, constituted the barrier of cooperative interaction between histoincompatible T and B cells. vi) Cooperation between H-2 compatible, non-syngeneic T and B cells was also disturbed in varying degrees depending on the strain combinations, thus indicating that cell surface antigens controlled by non-H-2 loci also had a significant role for the cooperative interaction between T and B cells. Problems associated with these findings were discussed.", "contents": "Histocompatibility and T-B cell cooperation in mouse radiation chimeras. Cooperative interaction between histocompatible as well as histoincompatible T and B cells in vivo was studied with B cell-bearing syngeneic radiation chimeras receiving syngeneic, semiallogeneic, or allogeneic A and T cells in various combinations. The results indicated the following: i) Histoincompatible T and B cells normally did not cooperate successfully for generation of antibody-forming cells. ii) Semiallogeneic (C3BF1) T cells cooperated successfully with parent-type (C3H) B cells developed in parent-type syngeneic chimeras (C3H/C3H), whereas parent-type (C3H as well as C57BL) T cells failed to cooperate with F1 (BC3F1) B cells developed in F1 syngeneic chimeras (BC3F1/BC3F1). iii) T cells obtained from C57BL/C3H or C57BL/C3BF1 chimeras, which were most likely donor (C57BL)- derived, cooperated successfully with C3H-derived B cells developed in C3H/C3H chimeras. iv) Evidence was obtained suggesting that stimulation of antibody response by allogeneic effect in the absence of syngeneic or semisyngeneic helper T cells did not take place in this experimental system. v) With the use of congenic resistant strains, it was shown that alloantigens controlled by H-2 loci, in particular by the K-I region of this gene complex, constituted the barrier of cooperative interaction between histoincompatible T and B cells. vi) Cooperation between H-2 compatible, non-syngeneic T and B cells was also disturbed in varying degrees depending on the strain combinations, thus indicating that cell surface antigens controlled by non-H-2 loci also had a significant role for the cooperative interaction between T and B cells. Problems associated with these findings were discussed."}
{"id": "PMID:1102604", "title": "Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.", "content": "The Cowan I strain of the bacterium Staphylococcus aureus has been used as an adsorbent for antibodies complexed with radiolabeled antigens from cell lysates. This application is advanced as a superior alternative to other methods of immune precipitation for the isolation of antigens. It exploits the high adsorption capacity for IgG molecules by protein A molecules on the cell walls of certain strains of staphylococci, along with the advantageous sedimentation properties of the bacteria. The interaction of immune complexes with the adsorbent was defined initially using a model system of bovine serum albumin with a high excess of rabbit anti-bovine serum albumin antibodies (IgG). The uptake of immune complexes under these conditions was extremely rapid, occurring within seconds, whereas maximum binding of free IgG was much slower. In addition, once bound the complexed antigen could not be displaced from the adsorbent either by large amounts of normal IgG or by extra free antibody. Antigen could be eluted almost completely from the inert adsorbent for analytic or preparative purposes with a variety of solvent systems, such as the detergent SDS in combination with urea and high temperature, and neutral salts with strong lyotropic salting in properties. The efficacy of the protein A-antibody adsorption technique was tested in direct comparisons with a conventional double antibody precipitation method for the isolation of mouse lymphocyte IgM. The bacterial adsorbent not only had a distinct advantage in speed of antigen isolation, but analyses by polyacrylamide gel electrophoresis in SDS also revealed consistently higher antigen recoveries, lower levels of background radioactivity, and an absence of other cell components which may nonspecifically bind to and complicate analyses using conventional immune precipitates.", "contents": "Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. The Cowan I strain of the bacterium Staphylococcus aureus has been used as an adsorbent for antibodies complexed with radiolabeled antigens from cell lysates. This application is advanced as a superior alternative to other methods of immune precipitation for the isolation of antigens. It exploits the high adsorption capacity for IgG molecules by protein A molecules on the cell walls of certain strains of staphylococci, along with the advantageous sedimentation properties of the bacteria. The interaction of immune complexes with the adsorbent was defined initially using a model system of bovine serum albumin with a high excess of rabbit anti-bovine serum albumin antibodies (IgG). The uptake of immune complexes under these conditions was extremely rapid, occurring within seconds, whereas maximum binding of free IgG was much slower. In addition, once bound the complexed antigen could not be displaced from the adsorbent either by large amounts of normal IgG or by extra free antibody. Antigen could be eluted almost completely from the inert adsorbent for analytic or preparative purposes with a variety of solvent systems, such as the detergent SDS in combination with urea and high temperature, and neutral salts with strong lyotropic salting in properties. The efficacy of the protein A-antibody adsorption technique was tested in direct comparisons with a conventional double antibody precipitation method for the isolation of mouse lymphocyte IgM. The bacterial adsorbent not only had a distinct advantage in speed of antigen isolation, but analyses by polyacrylamide gel electrophoresis in SDS also revealed consistently higher antigen recoveries, lower levels of background radioactivity, and an absence of other cell components which may nonspecifically bind to and complicate analyses using conventional immune precipitates."}
{"id": "PMID:1102605", "title": "Antigen suppression of the in vitro development of plaque-forming cells to autologous erythrocyte antigens.", "content": "Peritoneal cell cultures from NZB and C57BL/6 mice develop large numbers of PFC directed against antigens present on bromelain-treated isologous erythrocytes. The development of these autoimmune PFC can be suppressed by the addition of small numbers of BrMRBC at the start of the culture period. The possibility that the development of the plaque is prevented by the presence of antigen in vivo is discussed.", "contents": "Antigen suppression of the in vitro development of plaque-forming cells to autologous erythrocyte antigens. Peritoneal cell cultures from NZB and C57BL/6 mice develop large numbers of PFC directed against antigens present on bromelain-treated isologous erythrocytes. The development of these autoimmune PFC can be suppressed by the addition of small numbers of BrMRBC at the start of the culture period. The possibility that the development of the plaque is prevented by the presence of antigen in vivo is discussed."}
{"id": "PMID:1102606", "title": "Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes.", "content": "A variety of methods have been devised for the study of spontaneous and directed cell migration. Among these, the membrane filter method introduced by Boyden in 1962, with its more recent modifications, has become the technique of choice for studies of leukocyte migration in vitro. This method, however, cannot be applied without alteration to studies of chemotaxis and spontaneous migration of cells of different types. We describe in this report a new and simple method for studying human leukocyte chemotaxis, in vitro, which is based upon migration of cells under agarose gel. This method has application to both polymorphonuclear leukocytes and monocytes, permits measurement of both chemotaxis and spontaneous migration, requires fewer cells per test, and is rapid, simple, reproducible, and inexpensive to set up.", "contents": "Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. A variety of methods have been devised for the study of spontaneous and directed cell migration. Among these, the membrane filter method introduced by Boyden in 1962, with its more recent modifications, has become the technique of choice for studies of leukocyte migration in vitro. This method, however, cannot be applied without alteration to studies of chemotaxis and spontaneous migration of cells of different types. We describe in this report a new and simple method for studying human leukocyte chemotaxis, in vitro, which is based upon migration of cells under agarose gel. This method has application to both polymorphonuclear leukocytes and monocytes, permits measurement of both chemotaxis and spontaneous migration, requires fewer cells per test, and is rapid, simple, reproducible, and inexpensive to set up."}
{"id": "PMID:1102607", "title": "Desensitization: effects on cutaneous and peritoneal manifestations of delayed hypersensitivity in relation to lymphokine production.", "content": "Guinea pigs exhibiting delayed hypersensitivity may be desensitized by a single large dose of antigen administered intramuscularly. In the present experiments, desensitization with a soluble protein such as egg albumin (EA) or bovine gamma-globulin (BGG) was nonspecific in that they suppressed both cutaneous reactivity and the macrophage disappearance reaction (MDR) to unrelated antigen in doubly immunized animals. In contrast, myobacterial antigen, at the dose studied, showed limited specificity in that it could suppress the MDR but not a skin test to an unrelated antigen. Induction of an MDR was shown to be associated with the appearance of macrophage migration inhibitory factor (MIF) and chemotactic activity in the peritoneal exudate fluid. Pretreatment with desensitizing doses of mycobacteria prevented the EA-induced appearance of MIF in the peritoneal fluids. In contrast, chemotactic activity could still be recovered, even though the MDR was suppressed. Mycobacteria functioned as a specific desensitizer not only with respect to cutaneous reactivity, as stated above, but also with respect to the production of chemotatic factor by peritoneal exudate cells. In contrast, mycobacteria functioned as a nonspecific desensitizer with respect to both the MDR and MIF production. These results suggest that the MDR may be an in vivo manifestation of MIF activity, whereas the cutaneous delayed hypersensitivity reaction may be more dependent upon chemotactic activity. These observations reported here, taken in conjunction with previously described in vitro studies, suggest that desensitization is a complex phenomenon which may be the result of several different mechanisms acting separately or in concert. The apparent discrepancies in the literature as to whether or not desensitization is a specific process would appear to arise from differences in the experimental model and the particular antigen used.", "contents": "Desensitization: effects on cutaneous and peritoneal manifestations of delayed hypersensitivity in relation to lymphokine production. Guinea pigs exhibiting delayed hypersensitivity may be desensitized by a single large dose of antigen administered intramuscularly. In the present experiments, desensitization with a soluble protein such as egg albumin (EA) or bovine gamma-globulin (BGG) was nonspecific in that they suppressed both cutaneous reactivity and the macrophage disappearance reaction (MDR) to unrelated antigen in doubly immunized animals. In contrast, myobacterial antigen, at the dose studied, showed limited specificity in that it could suppress the MDR but not a skin test to an unrelated antigen. Induction of an MDR was shown to be associated with the appearance of macrophage migration inhibitory factor (MIF) and chemotactic activity in the peritoneal exudate fluid. Pretreatment with desensitizing doses of mycobacteria prevented the EA-induced appearance of MIF in the peritoneal fluids. In contrast, chemotactic activity could still be recovered, even though the MDR was suppressed. Mycobacteria functioned as a specific desensitizer not only with respect to cutaneous reactivity, as stated above, but also with respect to the production of chemotatic factor by peritoneal exudate cells. In contrast, mycobacteria functioned as a nonspecific desensitizer with respect to both the MDR and MIF production. These results suggest that the MDR may be an in vivo manifestation of MIF activity, whereas the cutaneous delayed hypersensitivity reaction may be more dependent upon chemotactic activity. These observations reported here, taken in conjunction with previously described in vitro studies, suggest that desensitization is a complex phenomenon which may be the result of several different mechanisms acting separately or in concert. The apparent discrepancies in the literature as to whether or not desensitization is a specific process would appear to arise from differences in the experimental model and the particular antigen used."}
{"id": "PMID:1102608", "title": "Interrelationships of surface immunoglobulin and Fc receptors on mouse B lymphocytes.", "content": "Receptors for the Fc portion of immunoglobulin (Ig) molecules have been detected on mouse B lymphocytes by an immunofluorescence technique, employing immune complexes of fluorescein-conjugated KLH and rabbit anti-KLH antibody. At 37 degrees C, membrane-bound complexes rapidly undergo capping and clearing from the surface. Modulation of Fc receptors does not alter the amount of distribution of surface Ig, indicating that these two molecules are independent moieties. However, capping of surface Ig by intact or pepsin-digested anti-Ig antibody leads to co-capping of Fc receptors. These results suggest that ligand binding to surface Ig induces some alteration which allows the Ig to enter into an association with Fc receptors.", "contents": "Interrelationships of surface immunoglobulin and Fc receptors on mouse B lymphocytes. Receptors for the Fc portion of immunoglobulin (Ig) molecules have been detected on mouse B lymphocytes by an immunofluorescence technique, employing immune complexes of fluorescein-conjugated KLH and rabbit anti-KLH antibody. At 37 degrees C, membrane-bound complexes rapidly undergo capping and clearing from the surface. Modulation of Fc receptors does not alter the amount of distribution of surface Ig, indicating that these two molecules are independent moieties. However, capping of surface Ig by intact or pepsin-digested anti-Ig antibody leads to co-capping of Fc receptors. These results suggest that ligand binding to surface Ig induces some alteration which allows the Ig to enter into an association with Fc receptors."}
{"id": "PMID:1102609", "title": "Functional deficiency of splenic adherent cells in New Zealand black mice.", "content": "New Zealand Black (NZB) mice hyporespond after in vitro immunization to sheep erythrocytes but not after in vivo immunization. The difference between in vitro and in vivo immunization was found to involve a non-antigen-specific change in the spleen which occurs in vivo soon after priming. Adherent spleen cells from young NZB or control strain mice were compared in their ability to cooperate with nonadherent cells in the induction of a primary immune response to sheep erythrocytes in vitro. The results support the hypothesis that there is a functional deficiency of adherent cells in the spleens of unprimed NZB mice.", "contents": "Functional deficiency of splenic adherent cells in New Zealand black mice. New Zealand Black (NZB) mice hyporespond after in vitro immunization to sheep erythrocytes but not after in vivo immunization. The difference between in vitro and in vivo immunization was found to involve a non-antigen-specific change in the spleen which occurs in vivo soon after priming. Adherent spleen cells from young NZB or control strain mice were compared in their ability to cooperate with nonadherent cells in the induction of a primary immune response to sheep erythrocytes in vitro. The results support the hypothesis that there is a functional deficiency of adherent cells in the spleens of unprimed NZB mice."}
{"id": "PMID:1102610", "title": "Enumerating immunoglobulin-secreting cells among peripheral human lymphocytes. A hemolytic plaque assay for a B cell function.", "content": "Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states.", "contents": "Enumerating immunoglobulin-secreting cells among peripheral human lymphocytes. A hemolytic plaque assay for a B cell function. Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states."}
{"id": "PMID:1102611", "title": "A new immunoassay based on fluorescence excitation by internal reflection spectroscopy.", "content": "A new immunoassay technique is described which uses totally internally reflected light to excite the fluorescence of fluorescein labeled antibody which has become bound to a hapten--protein conjugate absorbed on a quartz-plate in the antibody solution. The presence of any free hapten in solution reduces the amount of antibody free to bind to the surface and thus reduces the fluorescence signal. Measurement of the decrease of the fluorescent signal then gives a measure of the concentration of free hapten present. The technique is simple, fast and has high intrinsic sensitivity and specificity. It has been demonstrated for phenyl arsonic acid and morphine. Free morphine at a concentration of 2 X 10(-7) M is readily detected.", "contents": "A new immunoassay based on fluorescence excitation by internal reflection spectroscopy. A new immunoassay technique is described which uses totally internally reflected light to excite the fluorescence of fluorescein labeled antibody which has become bound to a hapten--protein conjugate absorbed on a quartz-plate in the antibody solution. The presence of any free hapten in solution reduces the amount of antibody free to bind to the surface and thus reduces the fluorescence signal. Measurement of the decrease of the fluorescent signal then gives a measure of the concentration of free hapten present. The technique is simple, fast and has high intrinsic sensitivity and specificity. It has been demonstrated for phenyl arsonic acid and morphine. Free morphine at a concentration of 2 X 10(-7) M is readily detected."}
{"id": "PMID:1102612", "title": "The L.A.I. microtest: a rapid and sensitive precedure for the demonstration of cell-mediated immunity in vitro.", "content": "A new microtest for cell-mediated immunity is described, which is based on the elaboration of a diffusible factor from sensitized lymphocytes following contact with specific antigen. The factor inhibits the adherence of neighbouring leucocytes to plastic surfaces. Production of the factor is ablated by pretreatment of lymphocytes with Theta-antiserum. It is suggested that the factor may be a new lymphokine, leucocyte adherence inhibition factor (LAIF).", "contents": "The L.A.I. microtest: a rapid and sensitive precedure for the demonstration of cell-mediated immunity in vitro. A new microtest for cell-mediated immunity is described, which is based on the elaboration of a diffusible factor from sensitized lymphocytes following contact with specific antigen. The factor inhibits the adherence of neighbouring leucocytes to plastic surfaces. Production of the factor is ablated by pretreatment of lymphocytes with Theta-antiserum. It is suggested that the factor may be a new lymphokine, leucocyte adherence inhibition factor (LAIF)."}
{"id": "PMID:1102614", "title": "Clinical trials with Alice strain, live, attenuated, serum inhibitor-resistant intranasal influenza A vaccine.", "content": "Two clinical trials with Alice strain intranasal influenza vaccine were performed. In study no. 1 (utilizing random selection and double-blind control), 50 subjects received a bivalent inactivated influenza vaccine intramuscularly, 99 subjects received Alice strain vaccine intranasally, and 50 subjects received a placebo intranasally. No symptomatology could be attributed to the intranasal route of immunization. Convalescent-phase geometric mean titers of hemagglutination inhibition antibody were higher after intramuscular vaccination; seroconversion occurred in 16 or 17 recipients of the Alice strain, with initial titers of less than 1:8. Clinical and virologic surveillance for 20 weeks after vaccination revealed no influenza A illnesses in participants of the study. In study no. 2, 75% of the subjects with initial nasal antibody titers of less than 1:3 developed measurable nasal antibody after receiving Alice strain vaccine.", "contents": "Clinical trials with Alice strain, live, attenuated, serum inhibitor-resistant intranasal influenza A vaccine. Two clinical trials with Alice strain intranasal influenza vaccine were performed. In study no. 1 (utilizing random selection and double-blind control), 50 subjects received a bivalent inactivated influenza vaccine intramuscularly, 99 subjects received Alice strain vaccine intranasally, and 50 subjects received a placebo intranasally. No symptomatology could be attributed to the intranasal route of immunization. Convalescent-phase geometric mean titers of hemagglutination inhibition antibody were higher after intramuscular vaccination; seroconversion occurred in 16 or 17 recipients of the Alice strain, with initial titers of less than 1:8. Clinical and virologic surveillance for 20 weeks after vaccination revealed no influenza A illnesses in participants of the study. In study no. 2, 75% of the subjects with initial nasal antibody titers of less than 1:3 developed measurable nasal antibody after receiving Alice strain vaccine."}
{"id": "PMID:1102616", "title": "Group B streptococcus in a general hospital.", "content": "Eighty-six percent of 707 beta-hemolytic streptococci isolated in a general hospital and excluded by presumptive tests from groups A and D were identified serologically as group B. More than 70% of the group B isolates were from urine cultures, the female genital gract, or newborn infants. Types III and II were the most common group B serotypes from most sources. However, types Ia, Ib, and Ic were more commonly isolated from the respiratory tract than from other sites, and type III was responsible for most serious neonatal infections. All group B streptococci were sensitive in vitro to comparable levels of penicillin G, ampicillin, and cephalothin and were highly resistant to kanamycin. Seventy-two percent were resistant to tetracycline but only 1%-2% to erythromycin, clindamycin, and chloramphenicol. Despite consistent sensitivity to penicillin G, the minimal inhibitory concentrations were significantly higher for group B than for group A streptococci.", "contents": "Group B streptococcus in a general hospital. Eighty-six percent of 707 beta-hemolytic streptococci isolated in a general hospital and excluded by presumptive tests from groups A and D were identified serologically as group B. More than 70% of the group B isolates were from urine cultures, the female genital gract, or newborn infants. Types III and II were the most common group B serotypes from most sources. However, types Ia, Ib, and Ic were more commonly isolated from the respiratory tract than from other sites, and type III was responsible for most serious neonatal infections. All group B streptococci were sensitive in vitro to comparable levels of penicillin G, ampicillin, and cephalothin and were highly resistant to kanamycin. Seventy-two percent were resistant to tetracycline but only 1%-2% to erythromycin, clindamycin, and chloramphenicol. Despite consistent sensitivity to penicillin G, the minimal inhibitory concentrations were significantly higher for group B than for group A streptococci."}
{"id": "PMID:1102617", "title": "Prevalence of toxigenic and invasive strains of Escherichia coli in a hospital population.", "content": "The pathogenesis of gastrointestinal infections caused by Escherichia coli has been attributed to the ability of some strains to produce exotoxins (enterotoxins) or to invade the intestinal mucosa directly. To investigate the possible role of invasiveness and of production of toxin in the pathogenesis of nonenteric E. coli infections, we studied E. coli isolated from 152 patients with urinary tract infections, bacteremia, or both. None of the isolates from urine or blood exhibited invasiveness in the guinea pig eye or produced toxin in tissue culture or in infant mice; only one patient was a gastrointestinal carrier of toxin-producing E. coli. We concluded that, in the population of this study, production of toxin and invasiveness were not important in the pathogenesis of most urinary tract infections or bacteremia caused by E. coli, and that gastrointestinal colonization with such organisms was uncommon.", "contents": "Prevalence of toxigenic and invasive strains of Escherichia coli in a hospital population. The pathogenesis of gastrointestinal infections caused by Escherichia coli has been attributed to the ability of some strains to produce exotoxins (enterotoxins) or to invade the intestinal mucosa directly. To investigate the possible role of invasiveness and of production of toxin in the pathogenesis of nonenteric E. coli infections, we studied E. coli isolated from 152 patients with urinary tract infections, bacteremia, or both. None of the isolates from urine or blood exhibited invasiveness in the guinea pig eye or produced toxin in tissue culture or in infant mice; only one patient was a gastrointestinal carrier of toxin-producing E. coli. We concluded that, in the population of this study, production of toxin and invasiveness were not important in the pathogenesis of most urinary tract infections or bacteremia caused by E. coli, and that gastrointestinal colonization with such organisms was uncommon."}
{"id": "PMID:1102620", "title": "The complement system in bullous pemphigoid. III. Fixation of C1q and C4 by pemphigoid antibody.", "content": "By in vitro complement immunofluorescent staining methods, 46 serum samples from bullous pemphigoid patients with titers of antibasement-membrane-zone antibodies of 320 or greater were tested for their ability to fix C1q, C4, and C3. All three staining reactions were positive with 23 of the samples; 21 samples yielded negative reactions. With one sample, the C1q and C3 staining reactions were positive but the C4 reaction was negative; one other sample gave a positive C3 staining reaction only. Absorption of anti-C1q, anti-C4, and anti-C3 with C1q, C4, and C3, respectively, blocked the specific in vitro complement staining. Therefore, bullous pemphigoid antibodies of the IgG type appear to be capable of activating the  \"classical\" complement pathway.", "contents": "The complement system in bullous pemphigoid. III. Fixation of C1q and C4 by pemphigoid antibody. By in vitro complement immunofluorescent staining methods, 46 serum samples from bullous pemphigoid patients with titers of antibasement-membrane-zone antibodies of 320 or greater were tested for their ability to fix C1q, C4, and C3. All three staining reactions were positive with 23 of the samples; 21 samples yielded negative reactions. With one sample, the C1q and C3 staining reactions were positive but the C4 reaction was negative; one other sample gave a positive C3 staining reaction only. Absorption of anti-C1q, anti-C4, and anti-C3 with C1q, C4, and C3, respectively, blocked the specific in vitro complement staining. Therefore, bullous pemphigoid antibodies of the IgG type appear to be capable of activating the  \"classical\" complement pathway."}
{"id": "PMID:1102621", "title": "Experimental aspergillus endocarditis in rabbits.", "content": "Aspergillus endocarditis in man usually occurs on prosthetic cardiac valves and gives rise to large vegetations which embolize easily producing peripheral organ infarction and infection. Blood cultures are usually sterile and the disease is difficult to cure with antimicrobial agents. Aspergillus endocarditis was studied in rabbits to determine the course, degree of fungemia, and response to treatment with amphotericin B (A), 5 flucytosine (5 FC) or A + 5 FC. Polyethylene tubing was introduced into the left ventricle through the carotid artery and 24 hours later animals were inoculated with 10(4) to 10(7) spores of a strain of Aspergillus fumigatus. Large occlusive vegetations developed on the aortic valves. Spontaneous mortality reached 67 per cent after 3 days. Despite large aggregates of mycelia seen beneath a layer of amorphous material on microscopic sections, vegetations contained only 10(3)  to 10(5) colony forming units (CFU) of aspergilli per gram, suggesting the aspergilli in tissues were clumped. Disseminated infection involving kidney, lung, liver, spleen, and brain occurred. Animals without intracardiac tubing which received the same inoculum of spores did not develop endocarditis, but showed evidence of disseminated infection. Blood after 24 hours of infection grew aspergilli only when large volumes were cultured and then only a small fraction of the total volume of blood obtained for culture yielded aspergilli, suggesting that the aspergilli in blood were clumped. Sterile vegetations in the absence of an intracardiac catheter were resistant to infection with aspergilli, but once established, infection with aspergilli persisted on vegetations despite removal of the catheter. Treatment of infected animals with A (1 mg. per kilogram), 5 FC (25 or 50 mg. per kilogram) or A + 5 FC daily intraperitoneally, significantly lowered the number of CFU per gram of vegetation.", "contents": "Experimental aspergillus endocarditis in rabbits. Aspergillus endocarditis in man usually occurs on prosthetic cardiac valves and gives rise to large vegetations which embolize easily producing peripheral organ infarction and infection. Blood cultures are usually sterile and the disease is difficult to cure with antimicrobial agents. Aspergillus endocarditis was studied in rabbits to determine the course, degree of fungemia, and response to treatment with amphotericin B (A), 5 flucytosine (5 FC) or A + 5 FC. Polyethylene tubing was introduced into the left ventricle through the carotid artery and 24 hours later animals were inoculated with 10(4) to 10(7) spores of a strain of Aspergillus fumigatus. Large occlusive vegetations developed on the aortic valves. Spontaneous mortality reached 67 per cent after 3 days. Despite large aggregates of mycelia seen beneath a layer of amorphous material on microscopic sections, vegetations contained only 10(3)  to 10(5) colony forming units (CFU) of aspergilli per gram, suggesting the aspergilli in tissues were clumped. Disseminated infection involving kidney, lung, liver, spleen, and brain occurred. Animals without intracardiac tubing which received the same inoculum of spores did not develop endocarditis, but showed evidence of disseminated infection. Blood after 24 hours of infection grew aspergilli only when large volumes were cultured and then only a small fraction of the total volume of blood obtained for culture yielded aspergilli, suggesting that the aspergilli in blood were clumped. Sterile vegetations in the absence of an intracardiac catheter were resistant to infection with aspergilli, but once established, infection with aspergilli persisted on vegetations despite removal of the catheter. Treatment of infected animals with A (1 mg. per kilogram), 5 FC (25 or 50 mg. per kilogram) or A + 5 FC daily intraperitoneally, significantly lowered the number of CFU per gram of vegetation."}
{"id": "PMID:1102622", "title": "Fatal haemorrhage from the innominate artery complicating tracheostomy.", "content": "A fatal haemorrhage of the innominate artery complicating tracheostomy for obstructive carcinoma of the larynx is reported. The possible mechanism of the rupture is discussed and methods of management are suggested.", "contents": "Fatal haemorrhage from the innominate artery complicating tracheostomy. A fatal haemorrhage of the innominate artery complicating tracheostomy for obstructive carcinoma of the larynx is reported. The possible mechanism of the rupture is discussed and methods of management are suggested."}
{"id": "PMID:1102624", "title": "Immunofluorescence studies on amphibian myoblast differentiation.", "content": "Differentiating myoblasts from urodele amphibians were cultivated in vitro and treated by immunofluorescence techniques and (or) inhibitors of RNA and protein syntheses. These experiments lead to the conclusion that the differentiation of these cells is directed by long-lived m-RNAs which are already present in morphologically undifferentiated cells and may act for at least 6 days. Contrary to observations on cultivated myogenic cells from other groups, the fusion of urodele myoblasts is not a prerequisite for a normal and functional differentiation (contractions) since isolated myoblasts differentiate quite normally. The appearance and further evolution of myosin and actin occur synchronously in this biological system.", "contents": "Immunofluorescence studies on amphibian myoblast differentiation. Differentiating myoblasts from urodele amphibians were cultivated in vitro and treated by immunofluorescence techniques and (or) inhibitors of RNA and protein syntheses. These experiments lead to the conclusion that the differentiation of these cells is directed by long-lived m-RNAs which are already present in morphologically undifferentiated cells and may act for at least 6 days. Contrary to observations on cultivated myogenic cells from other groups, the fusion of urodele myoblasts is not a prerequisite for a normal and functional differentiation (contractions) since isolated myoblasts differentiate quite normally. The appearance and further evolution of myosin and actin occur synchronously in this biological system."}
{"id": "PMID:1102625", "title": "The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs.", "content": "Nuclei from keratinized skin cells of adult Xenopus foot-webs have been transplanted to enucleated eggs of the same species. 2. The cells used to provide donor nuclei were obtained as a monolayer outgrowth from cultured foot-web explants. When explants were cultured without plasma for 3 days, over 99-9% of the outgrowth cells contained keratin as revealed by the binding of monospecific fluorescent antibody prepared against purified Xenopus keratin. Nuclei were transplanted from cells which had been cultured for 31/2 days. 3. None of the first transfer embryos developed as far as tadpoles. Eleven clones of embryos were prepared from the nuclei of partial first-transfer blastulae by use of serial nuclear transplantation. Eight of these clones contained swimming tadpoles with functional muscle and nerve cells, and six clones contained tadpoles with beating hearts, well differentiated eyes, and other organs. 4. To prove that the nuclei of nuclear-transplant tadpoles were derived from the transplanted skin cell nuclei and not from a failure of ultraviolet light to inactivate the recipient egg nucleus, 1-nu skin cell nuclei were transplanted to eggs laid by 2-nu frogs. Several advanced tadpoles from six clones were analysed for nucleolar and chromosome number and found to be 1-nu diploids. 5. The six clones of advanced tadpoles which were proved to carry the donor nuclear marker represent six first-transfer nuclei in a total sample of 129 skin cell nuclei originally transplanted. The probability that all six nuclei were derived from the 0-1% of the donor cell population not proved to contain keratin is less than one in 10(10). 6. We conclude that cell specialization does not involve any loss, irreversible inactivation or permanent change in chromosomal genes required for development.", "contents": "The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. Nuclei from keratinized skin cells of adult Xenopus foot-webs have been transplanted to enucleated eggs of the same species. 2. The cells used to provide donor nuclei were obtained as a monolayer outgrowth from cultured foot-web explants. When explants were cultured without plasma for 3 days, over 99-9% of the outgrowth cells contained keratin as revealed by the binding of monospecific fluorescent antibody prepared against purified Xenopus keratin. Nuclei were transplanted from cells which had been cultured for 31/2 days. 3. None of the first transfer embryos developed as far as tadpoles. Eleven clones of embryos were prepared from the nuclei of partial first-transfer blastulae by use of serial nuclear transplantation. Eight of these clones contained swimming tadpoles with functional muscle and nerve cells, and six clones contained tadpoles with beating hearts, well differentiated eyes, and other organs. 4. To prove that the nuclei of nuclear-transplant tadpoles were derived from the transplanted skin cell nuclei and not from a failure of ultraviolet light to inactivate the recipient egg nucleus, 1-nu skin cell nuclei were transplanted to eggs laid by 2-nu frogs. Several advanced tadpoles from six clones were analysed for nucleolar and chromosome number and found to be 1-nu diploids. 5. The six clones of advanced tadpoles which were proved to carry the donor nuclear marker represent six first-transfer nuclei in a total sample of 129 skin cell nuclei originally transplanted. The probability that all six nuclei were derived from the 0-1% of the donor cell population not proved to contain keratin is less than one in 10(10). 6. We conclude that cell specialization does not involve any loss, irreversible inactivation or permanent change in chromosomal genes required for development."}
{"id": "PMID:1102628", "title": "Intraosseous neurilemmoma of the cervical spine causing paraparesis and treated by resection and grafting.", "content": "A neurilemmoma presented as an expanding lesion of the bodies of C6 and C7 vertebrae and caused local neurological signs together with a spastic paraparesis. This tumour was treated by preliminary posterior fusion, followed by its complete removal via an anterior approach and stabilization by anterior spinal fusion. Other cases in the literature are reviewed and discussed.", "contents": "Intraosseous neurilemmoma of the cervical spine causing paraparesis and treated by resection and grafting. A neurilemmoma presented as an expanding lesion of the bodies of C6 and C7 vertebrae and caused local neurological signs together with a spastic paraparesis. This tumour was treated by preliminary posterior fusion, followed by its complete removal via an anterior approach and stabilization by anterior spinal fusion. Other cases in the literature are reviewed and discussed."}
{"id": "PMID:1102630", "title": "Blood flow in normal and injured monkey spinal cord.", "content": "The authors used indicator fractionation techniques to determine blood flow in normal and bluntly traumatized spinal cords of Macaca rhesus monkeys. Normal flow rates were determined for several levels of spinal cord as well as differential values for white and gray matter from representative areas. Flow rates in traumatized tissue, obtained at several different time intervals up to 4 hours after injury, demonstrated marked differences in regional perfusion of the white matter and gray matter after trauma. Gray matter perfusion was nearly obliterated while white matter blood flow persisted and in fact was higher than uninjured controls. The findings do not support the concept of ischemia as a factor in white matter failure. If toxic pathobiochemical alterations are induced by trauma, it may be possible to reverse these changes by exploiting the preserved white matter blood flow for chemotherapeutic intervention.", "contents": "Blood flow in normal and injured monkey spinal cord. The authors used indicator fractionation techniques to determine blood flow in normal and bluntly traumatized spinal cords of Macaca rhesus monkeys. Normal flow rates were determined for several levels of spinal cord as well as differential values for white and gray matter from representative areas. Flow rates in traumatized tissue, obtained at several different time intervals up to 4 hours after injury, demonstrated marked differences in regional perfusion of the white matter and gray matter after trauma. Gray matter perfusion was nearly obliterated while white matter blood flow persisted and in fact was higher than uninjured controls. The findings do not support the concept of ischemia as a factor in white matter failure. If toxic pathobiochemical alterations are induced by trauma, it may be possible to reverse these changes by exploiting the preserved white matter blood flow for chemotherapeutic intervention."}
{"id": "PMID:1102632", "title": "Our underpinnings: a bicentennial view. The 1975 Harvey Cushing oration.", "content": "The author describes the emergence of the liberal traditions which is the foundation of the political and economic systems in this country and considers policies that may affect our social and economic future.", "contents": "Our underpinnings: a bicentennial view. The 1975 Harvey Cushing oration. The author describes the emergence of the liberal traditions which is the foundation of the political and economic systems in this country and considers policies that may affect our social and economic future."}
{"id": "PMID:1102633", "title": "Systemic hypotension in neurosurgery.", "content": "The authors review the intraoperative use of elective hypotension to reduce the probability of hemorrhage, to increase pliability of the aneurysmal sac for ease of clip application, and to control hemorrhage. The optimum agent and techniques for lowering systemic blood pressure remain controversial, but trimethaphan, sodium nitroprusside, and halothane have been found most useful. When cerebral blood flow falls below the brain's capacity to autoregulate, distinct time-related alterations occur biochemically and histologically. The profile of prolonged reduced adenosine triphosphate (ATP), low phosphocreatine, low glucose, and elevated lactate and lactate/pyruvate ratio is associated with swelling of perivascular astrocytes and \"blebbing\" of vascular endothelial cells with subsequent cerebral damage. To prevent permanent alteration it is desirable to observe time constraints and to employ other means of protection such as hypothermia, although the authors believe the latter unnecessary for short hypotensive periods. It has been proposed, but not substantiated, that anesthetics which depress rate of cerebral oxygen consumption but do not affect cerebral ATP level protect the brain from hypotension. Several investigations suggest that halothane, a vasodiltor, satisfies the safety requirement. The most prominent contraindication to halothane, however, is elevation of intracranial pressure. At present hypotensive surgery for aneurysmorrhapy is usually performed when intracranial pressure has returned to normal. Experimentally the electroencephalogram has been observed to show alterations prior to biochemical parameters for following brain vulnerability, so that it conceivably could be an effective monitoring technique during prolonged profound hypotension.", "contents": "Systemic hypotension in neurosurgery. The authors review the intraoperative use of elective hypotension to reduce the probability of hemorrhage, to increase pliability of the aneurysmal sac for ease of clip application, and to control hemorrhage. The optimum agent and techniques for lowering systemic blood pressure remain controversial, but trimethaphan, sodium nitroprusside, and halothane have been found most useful. When cerebral blood flow falls below the brain's capacity to autoregulate, distinct time-related alterations occur biochemically and histologically. The profile of prolonged reduced adenosine triphosphate (ATP), low phosphocreatine, low glucose, and elevated lactate and lactate/pyruvate ratio is associated with swelling of perivascular astrocytes and \"blebbing\" of vascular endothelial cells with subsequent cerebral damage. To prevent permanent alteration it is desirable to observe time constraints and to employ other means of protection such as hypothermia, although the authors believe the latter unnecessary for short hypotensive periods. It has been proposed, but not substantiated, that anesthetics which depress rate of cerebral oxygen consumption but do not affect cerebral ATP level protect the brain from hypotension. Several investigations suggest that halothane, a vasodiltor, satisfies the safety requirement. The most prominent contraindication to halothane, however, is elevation of intracranial pressure. At present hypotensive surgery for aneurysmorrhapy is usually performed when intracranial pressure has returned to normal. Experimentally the electroencephalogram has been observed to show alterations prior to biochemical parameters for following brain vulnerability, so that it conceivably could be an effective monitoring technique during prolonged profound hypotension."}
{"id": "PMID:1102638", "title": "Acupuncture analgesia in dentistry: an analysis of studies from the People's Republic of China.", "content": "Studies conducted on the use of acupuncture analgesia for dental procedures in the People's Republic of China have not provided sufficient evidence to indicate that acupuncture analgesia can replace conventional drugs as the preferred anesthetic. However, studies currently in progress in China and in the United States may be successful in finding ways to reduce the failure rate and in determining the operative benefits of acupuncture.", "contents": "Acupuncture analgesia in dentistry: an analysis of studies from the People's Republic of China. Studies conducted on the use of acupuncture analgesia for dental procedures in the People's Republic of China have not provided sufficient evidence to indicate that acupuncture analgesia can replace conventional drugs as the preferred anesthetic. However, studies currently in progress in China and in the United States may be successful in finding ways to reduce the failure rate and in determining the operative benefits of acupuncture."}
{"id": "PMID:1102641", "title": "Possible nosocomial transmission of group B streptococci in a newborn nursery.", "content": "Within a six-day period in March, 1974, three infants born at a hospital in central Arkansas developed meningitis caused by group B, type III Streptococci. Three factors suggested nosocomial transmission of the organism in the nursery: (1) the three infants were born in a six-day period, (2) four weeks after their infants' births, none of the parents had positive cultures for group B streptococci, and (3) 31% of infants born in the hospital in March were colonized with group B, type III streptococci, while in April, after control measures in the nursery were instituted, only 2% of infants were colonized with this type (p less than 0.0002). Colonized infants were treated with penicillin, but follow-up cultures at two and six weeks showed that half the infants tested were still colonized. The number of personnel colonized with group B streptococci was not significantly different in personnel exposed to infants when compared with those that were not, and handwashing and environmental cultures were negative for group B streptococci. The results of this investigation give additional support to the concept that nosocomial transmission of group B streptococci can occur and may be effectively interrupted by control measures in the nursery.", "contents": "Possible nosocomial transmission of group B streptococci in a newborn nursery. Within a six-day period in March, 1974, three infants born at a hospital in central Arkansas developed meningitis caused by group B, type III Streptococci. Three factors suggested nosocomial transmission of the organism in the nursery: (1) the three infants were born in a six-day period, (2) four weeks after their infants' births, none of the parents had positive cultures for group B streptococci, and (3) 31% of infants born in the hospital in March were colonized with group B, type III streptococci, while in April, after control measures in the nursery were instituted, only 2% of infants were colonized with this type (p less than 0.0002). Colonized infants were treated with penicillin, but follow-up cultures at two and six weeks showed that half the infants tested were still colonized. The number of personnel colonized with group B streptococci was not significantly different in personnel exposed to infants when compared with those that were not, and handwashing and environmental cultures were negative for group B streptococci. The results of this investigation give additional support to the concept that nosocomial transmission of group B streptococci can occur and may be effectively interrupted by control measures in the nursery."}
{"id": "PMID:1102642", "title": "Treatment of Candida peritonitis by peritoneal lavage with amphotericin B.", "content": "A 14-year-old girl, who was a renal transplant recipient, developed Candida tropicalis peritonitis during peritoneal dialysis and immunosuppressive and broad-spectrum antibiotic therapy. Therapeutic cure of the peritonitis followed a ten-day course of amphotericin B administered solely by peritoneal lavage.", "contents": "Treatment of Candida peritonitis by peritoneal lavage with amphotericin B. A 14-year-old girl, who was a renal transplant recipient, developed Candida tropicalis peritonitis during peritoneal dialysis and immunosuppressive and broad-spectrum antibiotic therapy. Therapeutic cure of the peritonitis followed a ten-day course of amphotericin B administered solely by peritoneal lavage."}
{"id": "PMID:1102644", "title": "Congenital rubella.", "content": "The original concept of the rubella syndrome as a triad of abnormalities affecting the eye, heart, and hearing organs has changed dramatically in the past 30 years. This paper describes the various clinical manifestations of congenital rubella now recognized and the varied consequences of rubella virus infection upon the fetus. The pathogenesis of intrauterine rubella is discussed in relation to the immunologic response of the fetus and to possible mechanisms of cell and tissue damage. The need for preventive measures is discussed in relation to the total number of children damaged annually and the importance of multiple defects leading to severe handicaps. Active immunization procedures to prevent congenital rubella defects are reviewed, with comments on the differences between vaccination policies in the United Kingdom and in the United States. The importance of surveillance of congenital rubella defects is emphasized; details of the National Rubella Surveillance Scheme in the United Kingdom are discussed.", "contents": "Congenital rubella. The original concept of the rubella syndrome as a triad of abnormalities affecting the eye, heart, and hearing organs has changed dramatically in the past 30 years. This paper describes the various clinical manifestations of congenital rubella now recognized and the varied consequences of rubella virus infection upon the fetus. The pathogenesis of intrauterine rubella is discussed in relation to the immunologic response of the fetus and to possible mechanisms of cell and tissue damage. The need for preventive measures is discussed in relation to the total number of children damaged annually and the importance of multiple defects leading to severe handicaps. Active immunization procedures to prevent congenital rubella defects are reviewed, with comments on the differences between vaccination policies in the United Kingdom and in the United States. The importance of surveillance of congenital rubella defects is emphasized; details of the National Rubella Surveillance Scheme in the United Kingdom are discussed."}
{"id": "PMID:1102645", "title": "Nonspecific enhancers of resistance in man.", "content": "Nonspecific enhancers of resistance may include (1) viral interference, (2) interferon, (3) interferon inducers, (4) bacterial interference, (5) bacterial products such as Coley's \"toxins,\" endotoxins, or staphylococcal, BCG, and Corynebacterium parvum vaccines, (6) transfer factor, and (7) well-defined chemicals such as dinitrochlorbenzene, levamisole, and vitamin C. These are discussed only as they have been applied to man to learn whether or not they have enhanced his ability to resist infections and growth of tumors. Preliminary studies suggest that a variety of relatively safe and effective nonspecific enhancers may soon be available for clinical use.", "contents": "Nonspecific enhancers of resistance in man. Nonspecific enhancers of resistance may include (1) viral interference, (2) interferon, (3) interferon inducers, (4) bacterial interference, (5) bacterial products such as Coley's \"toxins,\" endotoxins, or staphylococcal, BCG, and Corynebacterium parvum vaccines, (6) transfer factor, and (7) well-defined chemicals such as dinitrochlorbenzene, levamisole, and vitamin C. These are discussed only as they have been applied to man to learn whether or not they have enhanced his ability to resist infections and growth of tumors. Preliminary studies suggest that a variety of relatively safe and effective nonspecific enhancers may soon be available for clinical use."}
{"id": "PMID:1102646", "title": "Immunologic and virologic studies in the postpericardiotomy syndrome.", "content": "A prospective, triple-blind study was undertaken to determine whether antiheart antibody or a rise in titer to a virus occurred in patients after intrapericardial surgery and, if so, whether either was related to clinical evidence of the postpericardiotomy syndrome. In 257 patients, AHA in high titer appeared in 62 (24%), all of whom had the syndrome. None of the 102 patients with no AHA had the syndrome. In 137 subjects, a rise in titer to one or more viral agents occurred in 21 of 31 (68%) of those with AHA and PPS. This study suggests that an immunologic response and viral illness are related to PPS.", "contents": "Immunologic and virologic studies in the postpericardiotomy syndrome. A prospective, triple-blind study was undertaken to determine whether antiheart antibody or a rise in titer to a virus occurred in patients after intrapericardial surgery and, if so, whether either was related to clinical evidence of the postpericardiotomy syndrome. In 257 patients, AHA in high titer appeared in 62 (24%), all of whom had the syndrome. None of the 102 patients with no AHA had the syndrome. In 137 subjects, a rise in titer to one or more viral agents occurred in 21 of 31 (68%) of those with AHA and PPS. This study suggests that an immunologic response and viral illness are related to PPS."}
{"id": "PMID:1102648", "title": "Rickets then and now.", "content": "Since the introduction of irradiated ergosterol into our food supply, nutritional vitamin D-deficiency rickets has become an uncommon disease. However, skeletal disorders due to abnormalities of vitamin D function still occur. These disorders can now be classified more exactly into two groups: those in which there is a deficiency of the active metabolite of vitamin D, 1,25-dihydroxyvitamin D, and those in which there is an abnormality of renal tubular function resulting in renal hypophosphatemia despite normal vitamin D metabolism. The various entities of these two groups are described and the theoretical basis of their treatment given.", "contents": "Rickets then and now. Since the introduction of irradiated ergosterol into our food supply, nutritional vitamin D-deficiency rickets has become an uncommon disease. However, skeletal disorders due to abnormalities of vitamin D function still occur. These disorders can now be classified more exactly into two groups: those in which there is a deficiency of the active metabolite of vitamin D, 1,25-dihydroxyvitamin D, and those in which there is an abnormality of renal tubular function resulting in renal hypophosphatemia despite normal vitamin D metabolism. The various entities of these two groups are described and the theoretical basis of their treatment given."}
{"id": "PMID:1102649", "title": "Failure to protect Mus musculus against Plasmodium berghei with footpad injections of killed parasites incorporated in complete Freund's adjuvant.", "content": "The stimulation of cell-mediated immunity by the injection of antigen from killed parasites incorporated in complete Freund's adjuvant did not protect Mus musculus from the erythrocytic stages of Plasmodium berghei. Agglutinating antibodies could not be detected, but delayed hypersensitivity was demonstrable. Apparently thymocyte sensitization alone was not sufficient to afford protection.", "contents": "Failure to protect Mus musculus against Plasmodium berghei with footpad injections of killed parasites incorporated in complete Freund's adjuvant. The stimulation of cell-mediated immunity by the injection of antigen from killed parasites incorporated in complete Freund's adjuvant did not protect Mus musculus from the erythrocytic stages of Plasmodium berghei. Agglutinating antibodies could not be detected, but delayed hypersensitivity was demonstrable. Apparently thymocyte sensitization alone was not sufficient to afford protection."}
{"id": "PMID:1102651", "title": "Thermodilution cardiac output measurement in infants and small children following intracardiac surgery.", "content": "A modified catheter has been used in conjunction with a thermodilution cardiac output computer for postoperative assessment of cardiac function in infants and children. Because of the small amount of fluid required for each determination and the simplicity of the technique, serial measurements can be done safely. Its use has contributed to the early detection of low output states. It has also proven to be a useful tool for estimating the optimal heart rate in each patient and for assessing the effectiveness of therapeutic measures.", "contents": "Thermodilution cardiac output measurement in infants and small children following intracardiac surgery. A modified catheter has been used in conjunction with a thermodilution cardiac output computer for postoperative assessment of cardiac function in infants and children. Because of the small amount of fluid required for each determination and the simplicity of the technique, serial measurements can be done safely. Its use has contributed to the early detection of low output states. It has also proven to be a useful tool for estimating the optimal heart rate in each patient and for assessing the effectiveness of therapeutic measures."}
{"id": "PMID:1102655", "title": "Osseous autografts. II. Histological responses to osseous coagulum-bone blend grafts.", "content": "Three human intraosseous lesions were treated using osseous coagulum-bone blend as graft material. These sites were surgically removed 6 to 13 weeks after treatment and the blocks prepared for histologic analysis. Periodontal remodeling at the site of grafting was noted in all specimens. This remodeling involved the osseous walls, periodontal ligament, cementum and graft spicules. Both osteoclastic and osteoblastic activities were seen at the borders of the spicules. Exfoliation of spicules was particularly frequent in the six-week specimen. Nevertheless, regenerating of both bone and cementum was actively taking place even in this early specimen and was still evident in the older specimens. Of particular interest was the apparent marked increase in cementogenesis at the graft sites and the variations in parallel or functional orientation of the periodontal ligament in these areas. Significant functional orientation of segments of the periodontal ligament were seenin the early specimens, but not in the latter one. The reason for such variations in ligament pattern is not known at this time.", "contents": "Osseous autografts. II. Histological responses to osseous coagulum-bone blend grafts. Three human intraosseous lesions were treated using osseous coagulum-bone blend as graft material. These sites were surgically removed 6 to 13 weeks after treatment and the blocks prepared for histologic analysis. Periodontal remodeling at the site of grafting was noted in all specimens. This remodeling involved the osseous walls, periodontal ligament, cementum and graft spicules. Both osteoclastic and osteoblastic activities were seen at the borders of the spicules. Exfoliation of spicules was particularly frequent in the six-week specimen. Nevertheless, regenerating of both bone and cementum was actively taking place even in this early specimen and was still evident in the older specimens. Of particular interest was the apparent marked increase in cementogenesis at the graft sites and the variations in parallel or functional orientation of the periodontal ligament in these areas. Significant functional orientation of segments of the periodontal ligament were seenin the early specimens, but not in the latter one. The reason for such variations in ligament pattern is not known at this time."}
{"id": "PMID:1102656", "title": "The effect of an antimicrobial mouthwash on dental plaque and gingivitis in yound adults.", "content": "The effectiveness of an antimicrobial mouthwash (alexidine, 2-ethylhexyl bisbiguanidine dihydrochloride) in preventing plaque and gingivitis was evaluated in 45 young male adults. In a study using a double-blind cross-over design, subjects received a thorough prophylaxis and refrained from all routine oral hygiene for 2 weeks. During this period, subjects rinsed twice daily with either a treatment (alexidine) or placebo mouthwash. Plaque and gingivitis were assessed on selected teeth on days 2, 7 and 14. The characteristics of plaque that were recorded include: surface area score, dry weight and differential counts of microorganisms. After the 14th day, subjects resumed their regular oral hygiene practices for 3 weeks before starting the second experimental period. Aftter a second prophylaxis, the treatment and placebo groups from the first 2 weeks were crossed over and the procedures followed previously were repeated during the sixth and seventh weeks. The alexidine mouthwash was statistically and clinically effective in decreasing plaque scores and plaque weight, and statistically but not clinically effective in decreasing gingivitis scores. No systemic side effects were observed, but an asymptomatic brown tongue stain was observed in about half of the subjects. There was no evidence to suggest that alexidine disturbed the relative composition of the microorganisms in plague, but there was a suggestion that it decreased the number of microorganisms almost 2-fold when compared with the placebo group.", "contents": "The effect of an antimicrobial mouthwash on dental plaque and gingivitis in yound adults. The effectiveness of an antimicrobial mouthwash (alexidine, 2-ethylhexyl bisbiguanidine dihydrochloride) in preventing plaque and gingivitis was evaluated in 45 young male adults. In a study using a double-blind cross-over design, subjects received a thorough prophylaxis and refrained from all routine oral hygiene for 2 weeks. During this period, subjects rinsed twice daily with either a treatment (alexidine) or placebo mouthwash. Plaque and gingivitis were assessed on selected teeth on days 2, 7 and 14. The characteristics of plaque that were recorded include: surface area score, dry weight and differential counts of microorganisms. After the 14th day, subjects resumed their regular oral hygiene practices for 3 weeks before starting the second experimental period. Aftter a second prophylaxis, the treatment and placebo groups from the first 2 weeks were crossed over and the procedures followed previously were repeated during the sixth and seventh weeks. The alexidine mouthwash was statistically and clinically effective in decreasing plaque scores and plaque weight, and statistically but not clinically effective in decreasing gingivitis scores. No systemic side effects were observed, but an asymptomatic brown tongue stain was observed in about half of the subjects. There was no evidence to suggest that alexidine disturbed the relative composition of the microorganisms in plague, but there was a suggestion that it decreased the number of microorganisms almost 2-fold when compared with the placebo group."}
{"id": "PMID:1102657", "title": "A new automated interpretation system for the 16PF.", "content": "A new computerized interpretation system for the 16PF is described. This system is based on the novel concept of attempting to simulate directly the diagnostic report-writing capabilities of a single clinician. Certain considerations regarding the problem of validity in respect to this area are brought up, and the general operation of the system is outlined.", "contents": "A new automated interpretation system for the 16PF. A new computerized interpretation system for the 16PF is described. This system is based on the novel concept of attempting to simulate directly the diagnostic report-writing capabilities of a single clinician. Certain considerations regarding the problem of validity in respect to this area are brought up, and the general operation of the system is outlined."}
{"id": "PMID:1102658", "title": "Perception of aggression and overt aggressive behavior.", "content": "Investigated the relationship between thresholds for perception of aggressive cues and overt aggressive behavior in 86 male undergraduate students. Overt aggressive behavior was measured by the amount of shock the subject attempted to inflict on his presumed opponent. It was found that individuals with low thresholds for aggressive cues expressed less overt aggressive behavior while individuals with high thresholds for aggression expressed more overt aggression. Thus an inverse relationship was found with respect to perceptual recognition thresholds for aggressive stimuli and overt aggressive behavior. The implications of these findings for projective techniques and their relevance to perception as it relates to overt behavior were discussed.", "contents": "Perception of aggression and overt aggressive behavior. Investigated the relationship between thresholds for perception of aggressive cues and overt aggressive behavior in 86 male undergraduate students. Overt aggressive behavior was measured by the amount of shock the subject attempted to inflict on his presumed opponent. It was found that individuals with low thresholds for aggressive cues expressed less overt aggressive behavior while individuals with high thresholds for aggression expressed more overt aggression. Thus an inverse relationship was found with respect to perceptual recognition thresholds for aggressive stimuli and overt aggressive behavior. The implications of these findings for projective techniques and their relevance to perception as it relates to overt behavior were discussed."}
{"id": "PMID:1102659", "title": "Microbial kinetics of drug action against gram-positive and gram-negative organisms. III: Effect of lincomycin and clindamycin combinations on Staphylococcus aureus and Escherichia coli.", "content": "The functional dependencies of apparent first-order generation rate constants kapp, of drug-affected cultures on drug concentrations indicate that lincomycin and clindamycin possess the same mechanism of action, which is bacteriostatic, against Staphylococcus aureus. Clindamycin also possesses another mechanism of action, which is bactericidal, at high concentration levels. However, clindamycin possesses only one of the two mechanisms of lincomycin action, which is bacteriostatic, against Escherichia coli. The relative potency of action of a clindamycin-lincomycin combination against Staph. aureus is variable, and the effective ratio ranges between 5:1 and 9:1; the effective ratio against E. coli is fixed at 6:1 over a wide concentration range. This difference is attributed to differences in bioavailability and/or binding characteristics of the drugs for bioreceptors, as a consequence of structural modifications in the drug molecules, and to differences in modes of action in the respective organisms. Mixtures containing equipotent fractions of clindamycin and lincomycin show \"equivalence\" or \"indifference\" of effects on Staph. aureus. The combined action of the mixtures can be quantitatively predicted from the separate dose-response curves of either component drug alone. Therefore, it is concluded that clindamycin and lincomycin may bind to the same receptor site that is engaged in microbial protein synthesis to inhibit the generation of Staph. aureus. However, combinations of clindamycin and lincomycin are less active than the a priori equipotent concentration of either drug alone in their action against E. coli, demonstrating unequivocally an antagonism of effects. Furthermore, the degree of antagonism is dependent on the order of addition of the drugs, which is attributed to the possibility that clindamycin and lincomycin bind differently on active and allosteric loci of the same receptor site functionally engaged in protein synthesis in E. coli. A rational approach to the quantification and prediction of combined antibiotic action must, therefore, be based not only on the kinetics and mechanisms of action as well as on the dose-response relationship over a wide concentration range for the separate antibiotics but also on the strain and species of the test organism.", "contents": "Microbial kinetics of drug action against gram-positive and gram-negative organisms. III: Effect of lincomycin and clindamycin combinations on Staphylococcus aureus and Escherichia coli. The functional dependencies of apparent first-order generation rate constants kapp, of drug-affected cultures on drug concentrations indicate that lincomycin and clindamycin possess the same mechanism of action, which is bacteriostatic, against Staphylococcus aureus. Clindamycin also possesses another mechanism of action, which is bactericidal, at high concentration levels. However, clindamycin possesses only one of the two mechanisms of lincomycin action, which is bacteriostatic, against Escherichia coli. The relative potency of action of a clindamycin-lincomycin combination against Staph. aureus is variable, and the effective ratio ranges between 5:1 and 9:1; the effective ratio against E. coli is fixed at 6:1 over a wide concentration range. This difference is attributed to differences in bioavailability and/or binding characteristics of the drugs for bioreceptors, as a consequence of structural modifications in the drug molecules, and to differences in modes of action in the respective organisms. Mixtures containing equipotent fractions of clindamycin and lincomycin show \"equivalence\" or \"indifference\" of effects on Staph. aureus. The combined action of the mixtures can be quantitatively predicted from the separate dose-response curves of either component drug alone. Therefore, it is concluded that clindamycin and lincomycin may bind to the same receptor site that is engaged in microbial protein synthesis to inhibit the generation of Staph. aureus. However, combinations of clindamycin and lincomycin are less active than the a priori equipotent concentration of either drug alone in their action against E. coli, demonstrating unequivocally an antagonism of effects. Furthermore, the degree of antagonism is dependent on the order of addition of the drugs, which is attributed to the possibility that clindamycin and lincomycin bind differently on active and allosteric loci of the same receptor site functionally engaged in protein synthesis in E. coli. A rational approach to the quantification and prediction of combined antibiotic action must, therefore, be based not only on the kinetics and mechanisms of action as well as on the dose-response relationship over a wide concentration range for the separate antibiotics but also on the strain and species of the test organism."}
{"id": "PMID:1102660", "title": "In vitro and in vivo availability of commercial prednisone tablets.", "content": "A three-way crossover bioavailability study was performed using nine adult male volunteers with three different commercial prednisone tablets. Plasma samples were assayed for prednisolone, the active metabolite of prednisone, by a radioimmunoassay method. Statistical analysis showed significant differences in the rate of appearance of prednisolone in plasma but not in the amount converted to prednisolone. The results suggest that differences in in vivo rates of appearance of prednisolone in plasma correlate with in vitro rates of dissolution.", "contents": "In vitro and in vivo availability of commercial prednisone tablets. A three-way crossover bioavailability study was performed using nine adult male volunteers with three different commercial prednisone tablets. Plasma samples were assayed for prednisolone, the active metabolite of prednisone, by a radioimmunoassay method. Statistical analysis showed significant differences in the rate of appearance of prednisolone in plasma but not in the amount converted to prednisolone. The results suggest that differences in in vivo rates of appearance of prednisolone in plasma correlate with in vitro rates of dissolution."}
{"id": "PMID:1102662", "title": "Opposing responses in sympathetic nerve activity induced by central injections of ouabain.", "content": "Cardiac glycosides induce changes in activity of peripheral sympathetic nerves. Arrhythmic doses have been suggested to act in the central nervous system to produce these changes, but direct central effects of digitalis on sympathetic discharge have not been documented. Thus, the central action of ouabain on sympathetic outflow was examined in baroreceptor and chemoreceptor denervated cats. Ouabain was injected into 128 vasoconstrictor or cardioaccelerator sites in the medulla or hypothalamus. Electrical stimulation of 24% of these sites evoked arrhythmias and stimulation consistently caused marked increases in heart rate, blood pressure and nerve activity, but ouabain had several effects, inducing either no change or increases or decreases in spontaneous activity of vasoconstrictor and cardioaccelerator nerves. Effects of ouabain were also observed on signal-averaged potentials evoked in these nerves by electrical stimulation of the medullary or hypothalamic sites of injection. The amplitude of the evoked potentials was either increased, decreased or left unchanged. In general, spontaneous and evoked activities were inhibited by ouabain more frequently than they were enhanced. The pattern of nervous responses to ouabain did not relate to the dose of drug or to the anatomical site of injection. Medullary and hypothalamic injections of ouabain often produced large changes in blood pressure, heart rate and nerve activity, but these effects were not accompanied by alterations in cardiac rhythm. Thus, central microinjections of ouabain produced heterogeneous patterns of effects on activity of peripheral sympathetic nerves, and these microinjections were not sufficient to evoke cardiac arrhythmias in cats with sectioned cranial nerves IX and X.", "contents": "Opposing responses in sympathetic nerve activity induced by central injections of ouabain. Cardiac glycosides induce changes in activity of peripheral sympathetic nerves. Arrhythmic doses have been suggested to act in the central nervous system to produce these changes, but direct central effects of digitalis on sympathetic discharge have not been documented. Thus, the central action of ouabain on sympathetic outflow was examined in baroreceptor and chemoreceptor denervated cats. Ouabain was injected into 128 vasoconstrictor or cardioaccelerator sites in the medulla or hypothalamus. Electrical stimulation of 24% of these sites evoked arrhythmias and stimulation consistently caused marked increases in heart rate, blood pressure and nerve activity, but ouabain had several effects, inducing either no change or increases or decreases in spontaneous activity of vasoconstrictor and cardioaccelerator nerves. Effects of ouabain were also observed on signal-averaged potentials evoked in these nerves by electrical stimulation of the medullary or hypothalamic sites of injection. The amplitude of the evoked potentials was either increased, decreased or left unchanged. In general, spontaneous and evoked activities were inhibited by ouabain more frequently than they were enhanced. The pattern of nervous responses to ouabain did not relate to the dose of drug or to the anatomical site of injection. Medullary and hypothalamic injections of ouabain often produced large changes in blood pressure, heart rate and nerve activity, but these effects were not accompanied by alterations in cardiac rhythm. Thus, central microinjections of ouabain produced heterogeneous patterns of effects on activity of peripheral sympathetic nerves, and these microinjections were not sufficient to evoke cardiac arrhythmias in cats with sectioned cranial nerves IX and X."}
{"id": "PMID:1102665", "title": "Critical factors in selecting removable prostheses.", "content": "Preservation of as many natural teeth as possible should be the goal of conscientious dentists. However, occasionally, the sacrifice of some sound natural teeth may be indicated for partially edentulous patients to provide them with the most functional and esthetic masticatory apparatus. Selection of the proper prosthetic service is one of the most critical value judgments made by dentists. No matter how skillfully the wrong treatment is accomplished, it is still the wrong treatment. It is important, therefore, that all relevant data be gathered so that the best clinical judgment can be brought to bear in the decision-making process.", "contents": "Critical factors in selecting removable prostheses. Preservation of as many natural teeth as possible should be the goal of conscientious dentists. However, occasionally, the sacrifice of some sound natural teeth may be indicated for partially edentulous patients to provide them with the most functional and esthetic masticatory apparatus. Selection of the proper prosthetic service is one of the most critical value judgments made by dentists. No matter how skillfully the wrong treatment is accomplished, it is still the wrong treatment. It is important, therefore, that all relevant data be gathered so that the best clinical judgment can be brought to bear in the decision-making process."}
{"id": "PMID:1102666", "title": "Minor connector designs for anterior acrylic resin bases: a preliminary study.", "content": "The effect of nine different designs on the strength of acrylic resin attachment to the partial denture framework in anterior endentulous spaces was tested. Thirty metal frameworks, incorporating nine different designs and a control, were fabricated. Acrylic resin was processed to these frameworks, and the resin was subjected to a shear force. The framework designs offering strong retention for acrylic resin bases allowed the use of a greater bulk of acrylic resin projecting through openings in the metal retention design.", "contents": "Minor connector designs for anterior acrylic resin bases: a preliminary study. The effect of nine different designs on the strength of acrylic resin attachment to the partial denture framework in anterior endentulous spaces was tested. Thirty metal frameworks, incorporating nine different designs and a control, were fabricated. Acrylic resin was processed to these frameworks, and the resin was subjected to a shear force. The framework designs offering strong retention for acrylic resin bases allowed the use of a greater bulk of acrylic resin projecting through openings in the metal retention design."}
{"id": "PMID:1102667", "title": "Clinical procedures for improving veneered metal crowns.", "content": "Satisfactory design and preparation of the veneer window presuppose that a perfect or a near-perfect preparation of the tooth has been made. The window is not just a cutout and is not the responsibility of the laboratory technician. Whenever optimum gingival health, a satisfactory esthetic result, and a long-lasting restoration are desired, it is worthwhile to carefully check and, if necessary, adjust the veneer window directly in the mouth before the veneer is added. This is especially important in restorations that extend below the gingival margin.", "contents": "Clinical procedures for improving veneered metal crowns. Satisfactory design and preparation of the veneer window presuppose that a perfect or a near-perfect preparation of the tooth has been made. The window is not just a cutout and is not the responsibility of the laboratory technician. Whenever optimum gingival health, a satisfactory esthetic result, and a long-lasting restoration are desired, it is worthwhile to carefully check and, if necessary, adjust the veneer window directly in the mouth before the veneer is added. This is especially important in restorations that extend below the gingival margin."}
{"id": "PMID:1102668", "title": "An adhesive technique for small anterior fixed partial dentures.", "content": "A technique for the fabrication of a resin anterior fixed partial denture of intermediate length has been described. This type of device offers an inexpensive, quick treatment for selected patients with two adjacent missing anterior teeth. The technique allows the postponement of a definitive treatment to a more favorable time. The total treatment can be completed in two relatively short appointments.", "contents": "An adhesive technique for small anterior fixed partial dentures. A technique for the fabrication of a resin anterior fixed partial denture of intermediate length has been described. This type of device offers an inexpensive, quick treatment for selected patients with two adjacent missing anterior teeth. The technique allows the postponement of a definitive treatment to a more favorable time. The total treatment can be completed in two relatively short appointments."}
{"id": "PMID:1102669", "title": "The provisional fixed partial denture.", "content": "A discussion of the rationale and requirements of the provisional restoration in fixed prosthetic dentistry has been presented. The imporatnce of this phase of restorative densitry and a survey of techniques for making interim restorations were gleaned from a review of the literature. A method for the fabrication of a provisional fixed partial denture using an acrylic resin interocclusal record was described and illustrated.", "contents": "The provisional fixed partial denture. A discussion of the rationale and requirements of the provisional restoration in fixed prosthetic dentistry has been presented. The imporatnce of this phase of restorative densitry and a survey of techniques for making interim restorations were gleaned from a review of the literature. A method for the fabrication of a provisional fixed partial denture using an acrylic resin interocclusal record was described and illustrated."}
{"id": "PMID:1102670", "title": "Movement of abutments by removable partial denture frameworks with a hemimaxillectomy obturator.", "content": "A laboratory experiment was conducted to measure the amount of movement in each of two directions on each of seven teeth, induced by three common designs of retainer, under both splinted and unsplinted conditions. An analysis of variance on a four-way, completely crossed, fixed-effects model showed significant main effects for design, splintedness, and teeth involved. Also of significance were the interactions between the factors of splintedness and teeth and between design and splintedness. Most of the variability in tooth movement was due to differences among the seven teeth (49.3 per cent of variance) and to the interaction of splintedness and teeth (20.2 per cent of variance).", "contents": "Movement of abutments by removable partial denture frameworks with a hemimaxillectomy obturator. A laboratory experiment was conducted to measure the amount of movement in each of two directions on each of seven teeth, induced by three common designs of retainer, under both splinted and unsplinted conditions. An analysis of variance on a four-way, completely crossed, fixed-effects model showed significant main effects for design, splintedness, and teeth involved. Also of significance were the interactions between the factors of splintedness and teeth and between design and splintedness. Most of the variability in tooth movement was due to differences among the seven teeth (49.3 per cent of variance) and to the interaction of splintedness and teeth (20.2 per cent of variance)."}
{"id": "PMID:1102671", "title": "Porcelain occlusal posterior fixed partial dentures.", "content": "A method for using the functionally generated-path technique to fabricate full occlusal porcelain-fused-to-metal fixed partial dentures and crowns has been described. Proper use of this technique in carefully selected patients should eliminate occlusal adjustments and the concomitant polishing and glazing that are often necessary before the restoration can be permanently cemented.", "contents": "Porcelain occlusal posterior fixed partial dentures. A method for using the functionally generated-path technique to fabricate full occlusal porcelain-fused-to-metal fixed partial dentures and crowns has been described. Proper use of this technique in carefully selected patients should eliminate occlusal adjustments and the concomitant polishing and glazing that are often necessary before the restoration can be permanently cemented."}
{"id": "PMID:1102683", "title": "Articular complications of homotransplantation and chronic renal hemodialysis.", "content": "Renal homotransplantaion and chronic hemodialysis are accepted methods of treating end-stage kidney disease. However, these procedures are fraught with complications involving bones, joints, and soft tissues. Transplantation and immunosuppressive therapy problems include \"connective tissue-like\" reactions, infections in joints and avascular necrosis of bone. Long term hemodialysis may accentuate secondary hyperparathyroidism, renal osteodystrophy, and metastatic calcification, which can be minimized by phosphorous control or calcium loading in the dialysate. In the presence of osteomalacia, vitamin D may be helpful and parathyroidectomy is indicated if autonomy is present. In one patient undergoing long term hemodialysis, a chalky material was aspirated from the olecranon bursa. Two inorganic solid phases were identified as being present - a major phase, octacalcium phosphate (ocp) and a minor phase, calcite (CaCO3). Because of its elusive properties, the role of OCP in biological systems is poorly known and can easily escape detection. Methods of identification of OCP and its potential role in crystal deposition syndromes are discussed.", "contents": "Articular complications of homotransplantation and chronic renal hemodialysis. Renal homotransplantaion and chronic hemodialysis are accepted methods of treating end-stage kidney disease. However, these procedures are fraught with complications involving bones, joints, and soft tissues. Transplantation and immunosuppressive therapy problems include \"connective tissue-like\" reactions, infections in joints and avascular necrosis of bone. Long term hemodialysis may accentuate secondary hyperparathyroidism, renal osteodystrophy, and metastatic calcification, which can be minimized by phosphorous control or calcium loading in the dialysate. In the presence of osteomalacia, vitamin D may be helpful and parathyroidectomy is indicated if autonomy is present. In one patient undergoing long term hemodialysis, a chalky material was aspirated from the olecranon bursa. Two inorganic solid phases were identified as being present - a major phase, octacalcium phosphate (ocp) and a minor phase, calcite (CaCO3). Because of its elusive properties, the role of OCP in biological systems is poorly known and can easily escape detection. Methods of identification of OCP and its potential role in crystal deposition syndromes are discussed."}
{"id": "PMID:1102684", "title": "The efficacy of antimalarials in systemic lupus erythematosus.", "content": "Of 209 patients who fulfilled the A.R.A. criteria for the diagnosis of systemic lupus erythematosus, 43 were selected for study because each had been treated for at least two years with antimalarials, but had not received antimalarials for at least one subsequent year. In each instance, the antimalarial was discontinued solely because of the development of retinopathy. Each year on antimalarials was matched with a subsequent year off antimalarials for each patient. The year immediately following diagnosis and years of pregnancy were excluded. Paired-t test analysis of matched years revealed that general symptoms (fever, fatigue, weight loss) were less common during years on 500 mg chloroquine daily than during years off the drug (p less than 0.05). Skin manifestations were also less frequent during the years on 500 mg chloroquine daily than during the years off (p less than 0.05). No significant steroid sparing effect was found. However, a greater incidence of flare-ups during the matched years off the drug was statistically significant.", "contents": "The efficacy of antimalarials in systemic lupus erythematosus. Of 209 patients who fulfilled the A.R.A. criteria for the diagnosis of systemic lupus erythematosus, 43 were selected for study because each had been treated for at least two years with antimalarials, but had not received antimalarials for at least one subsequent year. In each instance, the antimalarial was discontinued solely because of the development of retinopathy. Each year on antimalarials was matched with a subsequent year off antimalarials for each patient. The year immediately following diagnosis and years of pregnancy were excluded. Paired-t test analysis of matched years revealed that general symptoms (fever, fatigue, weight loss) were less common during years on 500 mg chloroquine daily than during years off the drug (p less than 0.05). Skin manifestations were also less frequent during the years on 500 mg chloroquine daily than during the years off (p less than 0.05). No significant steroid sparing effect was found. However, a greater incidence of flare-ups during the matched years off the drug was statistically significant."}
{"id": "PMID:1102688", "title": "Accountability for graduate medical education: report of an unsuccessful experiment.", "content": "An experiment is reported which utilized a professional medical care organization as the base for intern and resident education. The major reason for its failure was the undue rigidity of Title XVIII (Medicare) of the Social Security Act and associated regulations. The data assembled suggest that the mechanism is feasible and could potentially obviate (or at least minimize) many of the existing problems associated with the operation and financing of graduate medical education using a hospital as the organizational base.", "contents": "Accountability for graduate medical education: report of an unsuccessful experiment. An experiment is reported which utilized a professional medical care organization as the base for intern and resident education. The major reason for its failure was the undue rigidity of Title XVIII (Medicare) of the Social Security Act and associated regulations. The data assembled suggest that the mechanism is feasible and could potentially obviate (or at least minimize) many of the existing problems associated with the operation and financing of graduate medical education using a hospital as the organizational base."}
{"id": "PMID:1102718", "title": "An anatomical, histochemical, and autoradiographic study of the ever-growing molar dentition of Microtus with comments on the role of structure in growth and eruption.", "content": "An analysis of the microanatomy of the molar dentition of Microtus utilizing histological, histochemical and autoradiographic techniques reveals a complex architecture with distinctive morphogenic mechanisms which respond to the functional requirements of the organism. These mechanisms include; the maintenance of continued growth and eruption of the molars to compensate for continued hard tissue loss from wear at the occlusal surface of the crown throughout the entire lifespan of the orgainism and a positive feedback repair mechanism to protect the growth systems from the potential destruction this normal occlusal wear could initiate. An awareness and understanding of these phenomena is of significant value for interpreting palentological specimens and formulating a theoretical model for interpreting the evolution of Microtine molar dentitions.", "contents": "An anatomical, histochemical, and autoradiographic study of the ever-growing molar dentition of Microtus with comments on the role of structure in growth and eruption. An analysis of the microanatomy of the molar dentition of Microtus utilizing histological, histochemical and autoradiographic techniques reveals a complex architecture with distinctive morphogenic mechanisms which respond to the functional requirements of the organism. These mechanisms include; the maintenance of continued growth and eruption of the molars to compensate for continued hard tissue loss from wear at the occlusal surface of the crown throughout the entire lifespan of the orgainism and a positive feedback repair mechanism to protect the growth systems from the potential destruction this normal occlusal wear could initiate. An awareness and understanding of these phenomena is of significant value for interpreting palentological specimens and formulating a theoretical model for interpreting the evolution of Microtine molar dentitions."}
{"id": "PMID:1102719", "title": "Marked histoincompatibility between and within sublines of AKR mice used in a syngeneic leukemia model.", "content": "Sublines of AKR mice differed significantly in their histoincompatibility to AKR/J mice, as indicated by their ability to reject AKR/J skin grafts and an AKR transplantable leukemia. Further, all sublines tested demonstrated some degree of heterogeneity, because isografts were rejected in 5-97% of the mice, depending on the subline. The presence of excessive heterogeneity among and within sublines poses a serious problem for experimental oncologists using these sublines in their tumor models.", "contents": "Marked histoincompatibility between and within sublines of AKR mice used in a syngeneic leukemia model. Sublines of AKR mice differed significantly in their histoincompatibility to AKR/J mice, as indicated by their ability to reject AKR/J skin grafts and an AKR transplantable leukemia. Further, all sublines tested demonstrated some degree of heterogeneity, because isografts were rejected in 5-97% of the mice, depending on the subline. The presence of excessive heterogeneity among and within sublines poses a serious problem for experimental oncologists using these sublines in their tumor models."}
{"id": "PMID:1102720", "title": "The radioautographic transfer function and its implications for radioautographic methodology.", "content": "A function was developed to relate the radioautographic detectability of tritiated thymidine to its incorporation into nuclear DNA under various experimental and specimen processing conditions. The implications of this function for the interpretation of cell kinetic studies were outlined, and its implications for radioautographic methodology were discussed. The standardization of radioautographic methods for facilitation of comparisons of data obtained from different sources under dissimilar experimental conditions was discussed.", "contents": "The radioautographic transfer function and its implications for radioautographic methodology. A function was developed to relate the radioautographic detectability of tritiated thymidine to its incorporation into nuclear DNA under various experimental and specimen processing conditions. The implications of this function for the interpretation of cell kinetic studies were outlined, and its implications for radioautographic methodology were discussed. The standardization of radioautographic methods for facilitation of comparisons of data obtained from different sources under dissimilar experimental conditions was discussed."}
{"id": "PMID:1102721", "title": "The discrete phases of the cell cycle: autoradiographic, physical, and chemical evidences.", "content": "Recently the discrete model of the cell cycle, described as a series of metabolic progressions through four distinct phases, has been challenged by a few curves of percent labeled mitoses (PLM) and related autoradiographic data, which have been questionably interpreted in terms of a \"continuous\" cell cycle model. This conclusion is based in part on a few questionable observations which we summarize, along with our objections and explanations. We present a critical analysis of the autoradiographic data obtained with the PLM, in terms of the limitations and inconveniences of the technique itself. Some attention is paid to the usefulness of the \"radioautographic transfer function,\" which seems an arbitrary mathematical expression of old autoradiographic observations. Finally, a review is presented of the old (by autoradiography) and new (by physical, chemical, and computer image analysis) evidences of the existence of dicrete phases in the cell cycle, which in turn support the idea of an all-or-none phenomenon for DNA synthesis, and which rule out the hypothesis of a continuous cell cycle model with a varying DNA synthesis rate function.", "contents": "The discrete phases of the cell cycle: autoradiographic, physical, and chemical evidences. Recently the discrete model of the cell cycle, described as a series of metabolic progressions through four distinct phases, has been challenged by a few curves of percent labeled mitoses (PLM) and related autoradiographic data, which have been questionably interpreted in terms of a \"continuous\" cell cycle model. This conclusion is based in part on a few questionable observations which we summarize, along with our objections and explanations. We present a critical analysis of the autoradiographic data obtained with the PLM, in terms of the limitations and inconveniences of the technique itself. Some attention is paid to the usefulness of the \"radioautographic transfer function,\" which seems an arbitrary mathematical expression of old autoradiographic observations. Finally, a review is presented of the old (by autoradiography) and new (by physical, chemical, and computer image analysis) evidences of the existence of dicrete phases in the cell cycle, which in turn support the idea of an all-or-none phenomenon for DNA synthesis, and which rule out the hypothesis of a continuous cell cycle model with a varying DNA synthesis rate function."}
{"id": "PMID:1102722", "title": "A microradioimmunoassay for antibodies to tumor-associated antigens.", "content": "A versatile microradioimmunoassay for the detection of antibodies to tumor-associated and other tissue antigens was described. The method involved: a) the preparation of solid-phase antigen with cultured (already adhered) or noncultured cells (sedimented by centrifugation) fixed to Micro-Test plates with neutral buffered formaldehyde or absolute methanol; b) the incubation of the antigen with test or control sera; and c) the incubation of the antigen with radioiodinated antiglobulin antibody. The nonspecific background of radioactivity was reduced to an acceptable level by the fixed cells being precoated in the wells with 0.5% bovine serum albumin in phosphate-buffered saline which was also used for the dilution of sera and labeled antiglobulin antibody. Tumor cells in primary cultures gave a high background, as compared to long-term cultures, which was due to the presence of immunoglobulins (most likely tumor-specific antibody). The specific antibody response to a syngeneic mouse tumor was demonstrated by this technique.", "contents": "A microradioimmunoassay for antibodies to tumor-associated antigens. A versatile microradioimmunoassay for the detection of antibodies to tumor-associated and other tissue antigens was described. The method involved: a) the preparation of solid-phase antigen with cultured (already adhered) or noncultured cells (sedimented by centrifugation) fixed to Micro-Test plates with neutral buffered formaldehyde or absolute methanol; b) the incubation of the antigen with test or control sera; and c) the incubation of the antigen with radioiodinated antiglobulin antibody. The nonspecific background of radioactivity was reduced to an acceptable level by the fixed cells being precoated in the wells with 0.5% bovine serum albumin in phosphate-buffered saline which was also used for the dilution of sera and labeled antiglobulin antibody. Tumor cells in primary cultures gave a high background, as compared to long-term cultures, which was due to the presence of immunoglobulins (most likely tumor-specific antibody). The specific antibody response to a syngeneic mouse tumor was demonstrated by this technique."}
{"id": "PMID:1102730", "title": "Bladder neck contracture and urethral stricture as complications of renal transplantation.", "content": "From 1963 to July 26, 1974, 211 men underwent renal transplantation at our clinic. Urethral strictures were present prior to the initial transplant in 4 patients (1.8 per cent) and developed in the post-transplant period in 13 patients (6.1 per cent). Postoperative bladder neck contractures occurred in 2 patients (0.9 per cent). Repeated urethral dilations and surgical procedures on the lower urinary tract were generally well tolerated. Infectious problems were the most common secondary complications. No patient or allograft was lost as a direct result of the lower tract obstruction or its treatment. The greatest morbidity occurred in those patients who had a combination of urethral stricture and/or bladder neck contracture, uremia, previous or current immunosuppression and the absence of urine flowing through the urinary tract. This combination occurred most often in the patient who experienced failure of the first allograft and was being prepared for a second or third transplant. To avoid potentially serious urologic complications, male patients should have a repeat lower urinary tract evaluation and definitive correction of existing obstruction before re-transplantation is considered.", "contents": "Bladder neck contracture and urethral stricture as complications of renal transplantation. From 1963 to July 26, 1974, 211 men underwent renal transplantation at our clinic. Urethral strictures were present prior to the initial transplant in 4 patients (1.8 per cent) and developed in the post-transplant period in 13 patients (6.1 per cent). Postoperative bladder neck contractures occurred in 2 patients (0.9 per cent). Repeated urethral dilations and surgical procedures on the lower urinary tract were generally well tolerated. Infectious problems were the most common secondary complications. No patient or allograft was lost as a direct result of the lower tract obstruction or its treatment. The greatest morbidity occurred in those patients who had a combination of urethral stricture and/or bladder neck contracture, uremia, previous or current immunosuppression and the absence of urine flowing through the urinary tract. This combination occurred most often in the patient who experienced failure of the first allograft and was being prepared for a second or third transplant. To avoid potentially serious urologic complications, male patients should have a repeat lower urinary tract evaluation and definitive correction of existing obstruction before re-transplantation is considered."}
{"id": "PMID:1102731", "title": "Lymphocyst: ultrasound diagnosis and urologic management.", "content": "Three cases of lymphocysts occurred in a series of 97 patients undergoing extension pelvic urological operations, an incidence rate of 3.1 per cent. This is the first report of this complication after urological lymphadenectomy. Awareness of the entity is important to urologists since lymphatic staging operations for prostate and bladder carcinoma are widely used to help plan definitive therapy. Athough this complication is rare after renal transplantation it may cause significant interference with graft function. The etiology, symptoms, findings, diagnosis and treatment of this condition are reviewed. Echography is presented as an ideal technique to diagnose the condition and to guide in its management.", "contents": "Lymphocyst: ultrasound diagnosis and urologic management. Three cases of lymphocysts occurred in a series of 97 patients undergoing extension pelvic urological operations, an incidence rate of 3.1 per cent. This is the first report of this complication after urological lymphadenectomy. Awareness of the entity is important to urologists since lymphatic staging operations for prostate and bladder carcinoma are widely used to help plan definitive therapy. Athough this complication is rare after renal transplantation it may cause significant interference with graft function. The etiology, symptoms, findings, diagnosis and treatment of this condition are reviewed. Echography is presented as an ideal technique to diagnose the condition and to guide in its management."}
{"id": "PMID:1102732", "title": "Rapid miniaturized tests for bacteriuria: Microstix and bacturcult urine tests.", "content": "Of 4 methods for testing random urine samples for the presence of infection the standard hospital laboratory culture-colony counting was found by far to be the most accurate. Microscopy of urinary sediment, Microstix and Bacturcult produced too many falsely negative results to be useful in a clinical setting, were urine samples were collected throughout the day such as in an office or hospital outpatient clinic. Perhaps the last 2 tests would yield better results if urine samples were collected after at least 4 hours of incubation time in the bladder, as suggested by their proponents.", "contents": "Rapid miniaturized tests for bacteriuria: Microstix and bacturcult urine tests. Of 4 methods for testing random urine samples for the presence of infection the standard hospital laboratory culture-colony counting was found by far to be the most accurate. Microscopy of urinary sediment, Microstix and Bacturcult produced too many falsely negative results to be useful in a clinical setting, were urine samples were collected throughout the day such as in an office or hospital outpatient clinic. Perhaps the last 2 tests would yield better results if urine samples were collected after at least 4 hours of incubation time in the bladder, as suggested by their proponents."}
{"id": "PMID:1102733", "title": "Recurrence of the nephrotic syndrome in the renal isograft: A preliminary report.", "content": "The fifth case of kidney transplantation between monozygotic twins in Japan is presented. Immediately after the transplanation the original disease, nephrotic syndrome, recurred but it was treated with steroids successfully. There have been few cases reported in which recurrence of the original disease in the isograft was treated successfully.", "contents": "Recurrence of the nephrotic syndrome in the renal isograft: A preliminary report. The fifth case of kidney transplantation between monozygotic twins in Japan is presented. Immediately after the transplanation the original disease, nephrotic syndrome, recurred but it was treated with steroids successfully. There have been few cases reported in which recurrence of the original disease in the isograft was treated successfully."}
{"id": "PMID:1102735", "title": "Cerebral blood volume in man. Computer analysis of a computerized brain scan.", "content": "Cerebral blood volume in man can be measured by subtracting computerized axial tomogram density measurements taken before from those taken after an intravascular marker is infused into the blood. The average blood volume is 3.0 ml/100 ml of brain tissue, with a range of 2.4 to 4.25. This method can be applied in studying cerebral pathologic conditions. Areas of cerebral edema, surrounding a tumor, have a decreased blood volume.", "contents": "Cerebral blood volume in man. Computer analysis of a computerized brain scan. Cerebral blood volume in man can be measured by subtracting computerized axial tomogram density measurements taken before from those taken after an intravascular marker is infused into the blood. The average blood volume is 3.0 ml/100 ml of brain tissue, with a range of 2.4 to 4.25. This method can be applied in studying cerebral pathologic conditions. Areas of cerebral edema, surrounding a tumor, have a decreased blood volume."}
{"id": "PMID:1102736", "title": "Hemoglobinopathy with prolonged bactermia. A report of two cases.", "content": "Prolonged Escherichia coli bacteremia occurred as a complication of pyelonephritis in two patients with abnormal hemoglobins (SC and SS), despite \"appropriate\" antibiotic therapy. Careful investigation in each case failed to account for the persistent sepsis. Pyogenic arthritis ultimately developed in both patients.", "contents": "Hemoglobinopathy with prolonged bactermia. A report of two cases. Prolonged Escherichia coli bacteremia occurred as a complication of pyelonephritis in two patients with abnormal hemoglobins (SC and SS), despite \"appropriate\" antibiotic therapy. Careful investigation in each case failed to account for the persistent sepsis. Pyogenic arthritis ultimately developed in both patients."}
{"id": "PMID:1102738", "title": "Listeria sepsis and meningitis: A complication of renal transplantation.", "content": "Listeria infections is an important cause of sepsis and meningitis in renal transplant patients. Prompt recognition and aggressive therapy are required to control this life-threatening infection. In 1972, one case of listeriosis occurred in a renal transplant recipient in our center; 1974, six more cases were detected within a period of nine months. All seven patients had Listeria sepsis, and three of the seven patients had both sepsis and meningitis. Antibiotic treatment resulted in remarkable improvement in the clinical state of all the patients in this study. Two patients later died of other unrelated causes.", "contents": "Listeria sepsis and meningitis: A complication of renal transplantation. Listeria infections is an important cause of sepsis and meningitis in renal transplant patients. Prompt recognition and aggressive therapy are required to control this life-threatening infection. In 1972, one case of listeriosis occurred in a renal transplant recipient in our center; 1974, six more cases were detected within a period of nine months. All seven patients had Listeria sepsis, and three of the seven patients had both sepsis and meningitis. Antibiotic treatment resulted in remarkable improvement in the clinical state of all the patients in this study. Two patients later died of other unrelated causes."}
{"id": "PMID:1102746", "title": "Disseminated aspergillosis in a renal transplant patient: Diagnostic difficulties re-emphasized.", "content": "An asymptomatic and radiographically occult lung abscess was the primary focus of infection in this case of fatal disseminated aspergillosis in a renal transplant recipient. Extensive neurological evaluation in response to a change in personality failed to reveal a brain abscess, which was the cause of death. This case illustrates the variability in presentations of aspergillosis and the continuing difficulties in diagnosing this infection in immunosuppressed patients.", "contents": "Disseminated aspergillosis in a renal transplant patient: Diagnostic difficulties re-emphasized. An asymptomatic and radiographically occult lung abscess was the primary focus of infection in this case of fatal disseminated aspergillosis in a renal transplant recipient. Extensive neurological evaluation in response to a change in personality failed to reveal a brain abscess, which was the cause of death. This case illustrates the variability in presentations of aspergillosis and the continuing difficulties in diagnosing this infection in immunosuppressed patients."}
{"id": "PMID:1102750", "title": "[Methods of calculation of functional volumes of the heart ventricle].", "content": "The method of thermodilution permitted a broader investigation of the methods of calculations of the functional heart volumes. Two methods (Holt and Luthy) were compared with one another and with the control. Both methods were used for calculating the end-diastolic volume (Qed). Studies in the calorimeter and on a model with a pulsating flow demonstrated that the first portion of the thermodilution curve is a function of the myocardial contractility. Both the Holt and Luthy methods give equally reliable results, but the Holt technique is preferable for Qed measurements.", "contents": "[Methods of calculation of functional volumes of the heart ventricle]. The method of thermodilution permitted a broader investigation of the methods of calculations of the functional heart volumes. Two methods (Holt and Luthy) were compared with one another and with the control. Both methods were used for calculating the end-diastolic volume (Qed). Studies in the calorimeter and on a model with a pulsating flow demonstrated that the first portion of the thermodilution curve is a function of the myocardial contractility. Both the Holt and Luthy methods give equally reliable results, but the Holt technique is preferable for Qed measurements."}
{"id": "PMID:1102751", "title": "[Simplified method of radiocardiographic study of central hemodynamics].", "content": "The possibility of calculating the main parameters of the cardiac output and the pulmonary blood flow on the basis of a radiocardiogram alone, without performing radiometry of the administered dose of the isotope, and determination of the venous blood activity, is discussed. It is offered to use the relative indices of hemodynamics for this purpose: the indices expressing the values of the minute and stroke volumes, the circulating blood volume in the lungs expressed as percentage of the total circulating blood volume. A high degree of correlation between the absolute and relative indices of the central hemodynamics was established, the advantages and disadvantages of the simplied method of radiocardiography are revealed. The rationale of a parallel determination of the absolute and relative values of the hemodynamic parameters is emphasized since it permits a deeper insight into the hemodynamic shifts.", "contents": "[Simplified method of radiocardiographic study of central hemodynamics]. The possibility of calculating the main parameters of the cardiac output and the pulmonary blood flow on the basis of a radiocardiogram alone, without performing radiometry of the administered dose of the isotope, and determination of the venous blood activity, is discussed. It is offered to use the relative indices of hemodynamics for this purpose: the indices expressing the values of the minute and stroke volumes, the circulating blood volume in the lungs expressed as percentage of the total circulating blood volume. A high degree of correlation between the absolute and relative indices of the central hemodynamics was established, the advantages and disadvantages of the simplied method of radiocardiography are revealed. The rationale of a parallel determination of the absolute and relative values of the hemodynamic parameters is emphasized since it permits a deeper insight into the hemodynamic shifts."}
{"id": "PMID:1102752", "title": "[Selection of a rational method of calculation of minute blood volume by the dye dilution curves].", "content": "In calculating the cardiac output on the basis of the dye dilution curves it is important to determine the area of the first circulation of the dye. The existing techniques of the determination of this area are different in their complexity and precision of the obtained results. A comparative evaluation of 14 techniques or their modifications for the determination of the cardiac output according to the Evans blue dilution curves was undertaken in 40 subjects (normal individuals and patients with chronic pulmonary pathology). A new method of calculations of the area by two triangles is suggested. The most precise techniques appeared to be the planimetric method of exponential extrapolation, the two and five triangles methods introduced by the authors, as well as the calculations of Hamilton and Simpson. It seems most rational to use the two and five triangles methods that are not only precise, but also simple. The method with five rectangular triangles should be preferred since it does not require any special equipment. In exceptional cases, in which the recirculation wave begins early and/or the direction of the descending portion is not clear, the Dow equation may be recommended, with the results multiplied by the factor of 1.26.", "contents": "[Selection of a rational method of calculation of minute blood volume by the dye dilution curves]. In calculating the cardiac output on the basis of the dye dilution curves it is important to determine the area of the first circulation of the dye. The existing techniques of the determination of this area are different in their complexity and precision of the obtained results. A comparative evaluation of 14 techniques or their modifications for the determination of the cardiac output according to the Evans blue dilution curves was undertaken in 40 subjects (normal individuals and patients with chronic pulmonary pathology). A new method of calculations of the area by two triangles is suggested. The most precise techniques appeared to be the planimetric method of exponential extrapolation, the two and five triangles methods introduced by the authors, as well as the calculations of Hamilton and Simpson. It seems most rational to use the two and five triangles methods that are not only precise, but also simple. The method with five rectangular triangles should be preferred since it does not require any special equipment. In exceptional cases, in which the recirculation wave begins early and/or the direction of the descending portion is not clear, the Dow equation may be recommended, with the results multiplied by the factor of 1.26."}
{"id": "PMID:1102765", "title": "[Symptomatology, differential diagnosis and treatment of bacterial meningitis of newborns and infants (author's transl)].", "content": "Bacterial meningitis in our hospital too shows its maximal frequence with 30.5% in the first month of life, 25.2% of these cases being newborns. The frequent difficulties of diagnosis in this period of life are demonstrated. Predisposing factors and possible complications of meningitis are named. The principles of modern therapy are summarized.", "contents": "[Symptomatology, differential diagnosis and treatment of bacterial meningitis of newborns and infants (author's transl)]. Bacterial meningitis in our hospital too shows its maximal frequence with 30.5% in the first month of life, 25.2% of these cases being newborns. The frequent difficulties of diagnosis in this period of life are demonstrated. Predisposing factors and possible complications of meningitis are named. The principles of modern therapy are summarized."}
{"id": "PMID:1102767", "title": "Immunohistochemical evidence for the presence of collagen type III in human arterial walls, arterial thrombi, and in leukocytes, incubated with collagen in vitro.", "content": "Sections of arterial walls and of thrombi and smears of leukocytes previously incubated in vitro with collagen type III were examined by immunohistochemical technique for the presence of collagen types I, II and III. In arterial walls collagen type III was detected immediately underlaying the endothelial cell layer and in the tissue between tunica elastica interna and adventitia. Collagen type I was not shown in the subendothelial layer. Fresh thrombi contained occasionally collagen, but only of type III. This was associated with leukocytes. Leukocytes were capable in vitro to associate and/or phagocytose collagen type III and this could be visualized immunohistochemically. The data show that collagen type III in vivo may play a crucial role in the initiation of thrombus formation.", "contents": "Immunohistochemical evidence for the presence of collagen type III in human arterial walls, arterial thrombi, and in leukocytes, incubated with collagen in vitro. Sections of arterial walls and of thrombi and smears of leukocytes previously incubated in vitro with collagen type III were examined by immunohistochemical technique for the presence of collagen types I, II and III. In arterial walls collagen type III was detected immediately underlaying the endothelial cell layer and in the tissue between tunica elastica interna and adventitia. Collagen type I was not shown in the subendothelial layer. Fresh thrombi contained occasionally collagen, but only of type III. This was associated with leukocytes. Leukocytes were capable in vitro to associate and/or phagocytose collagen type III and this could be visualized immunohistochemically. The data show that collagen type III in vivo may play a crucial role in the initiation of thrombus formation."}
{"id": "PMID:1102776", "title": "Pancreatic beta cell changes induced by cyproheptadine in vitro.", "content": "The effect of cyproheptadine on the structure and function of rat pancreatic beta cells was studied in vitro by culturing isolated islets in media containing the drug (0.5 mM). After 8 days in culture the ultrastructure of islet cells maintained in control media appeared well preserved, being similar to the previously reported for islets kept in long term organ culture. In contrast beta cells from islets incubated in media containing cyproheptadine appeared degranulated and the rough endoplasmic reticulum showed cisternal dilation and vacuole formation. These vacuoles were filled with an electron-dense granular material and their surface was usually studded with ribosomes. These lesions are identical with those produced by the administration of cyproheptadine to rats in vivo. In addition to these findings, increased numbers of lysosomes and myeloid bodies were observed in both alpha and beta cells. Compared with that of the controls, the 24-hour basal insulin secretion of islets cultured in the presence of cyproheptadine was significantly reduced from the 4th day of the study onwards. It is thus concluded that cyproheptadine has direct effects on the morphology and function of the rat pancreatic beta cell.", "contents": "Pancreatic beta cell changes induced by cyproheptadine in vitro. The effect of cyproheptadine on the structure and function of rat pancreatic beta cells was studied in vitro by culturing isolated islets in media containing the drug (0.5 mM). After 8 days in culture the ultrastructure of islet cells maintained in control media appeared well preserved, being similar to the previously reported for islets kept in long term organ culture. In contrast beta cells from islets incubated in media containing cyproheptadine appeared degranulated and the rough endoplasmic reticulum showed cisternal dilation and vacuole formation. These vacuoles were filled with an electron-dense granular material and their surface was usually studded with ribosomes. These lesions are identical with those produced by the administration of cyproheptadine to rats in vivo. In addition to these findings, increased numbers of lysosomes and myeloid bodies were observed in both alpha and beta cells. Compared with that of the controls, the 24-hour basal insulin secretion of islets cultured in the presence of cyproheptadine was significantly reduced from the 4th day of the study onwards. It is thus concluded that cyproheptadine has direct effects on the morphology and function of the rat pancreatic beta cell."}
{"id": "PMID:1102777", "title": "Age dependence of viral expression. Electron microscopic and immunoperoxidase studies of Measles virus replication in mice.", "content": "The hamster neurotropic strain of measles virus inoculated intracerebrally into BALB/c mice causes a uniformly fatal encephalitis with production of infectious virus in 1- to 2-day-old suckling mice but a 31 per cent mortality with production of measles virus antigen without production of infectious virus in 4-week-weanling mice (8). These two groups of animals were studied using routine electron microscopy and the immunoperoxidase technique in order to determine the nature of the measles virus antigen produced in each situation. Suckling animals showed numerous cytoplasmic viral inclusions in neurons and glia. This nucleocapsid material stained specifically with rabbit antiserum to measles virus antigen overlayed with peroxidase-conjugated goat antirabbit gamma-globulin. Histologic and electron microscopic study of weanling mice revealed rare necrotic cells and occasional loose clusters of neurons with depolymerized ribosomes. Immunoperoxidase staining showed diffuse cytoplasmic staining of similar groups of neurons and dendritic processes without evidence of nucleocapsid material. These differences in virus replication appear to be a function of the maturation of the host cell rather than the measles virus. Hamster neurotropic virus infection of the weanling mouse is an example of a potentially fatal virus infection in which viral replication is defective at a stage prior to assembly of nucleocapsid material; thus, no direct morphologic evidence that the cause of the clinical disease is a viral infection is present.", "contents": "Age dependence of viral expression. Electron microscopic and immunoperoxidase studies of Measles virus replication in mice. The hamster neurotropic strain of measles virus inoculated intracerebrally into BALB/c mice causes a uniformly fatal encephalitis with production of infectious virus in 1- to 2-day-old suckling mice but a 31 per cent mortality with production of measles virus antigen without production of infectious virus in 4-week-weanling mice (8). These two groups of animals were studied using routine electron microscopy and the immunoperoxidase technique in order to determine the nature of the measles virus antigen produced in each situation. Suckling animals showed numerous cytoplasmic viral inclusions in neurons and glia. This nucleocapsid material stained specifically with rabbit antiserum to measles virus antigen overlayed with peroxidase-conjugated goat antirabbit gamma-globulin. Histologic and electron microscopic study of weanling mice revealed rare necrotic cells and occasional loose clusters of neurons with depolymerized ribosomes. Immunoperoxidase staining showed diffuse cytoplasmic staining of similar groups of neurons and dendritic processes without evidence of nucleocapsid material. These differences in virus replication appear to be a function of the maturation of the host cell rather than the measles virus. Hamster neurotropic virus infection of the weanling mouse is an example of a potentially fatal virus infection in which viral replication is defective at a stage prior to assembly of nucleocapsid material; thus, no direct morphologic evidence that the cause of the clinical disease is a viral infection is present."}
{"id": "PMID:1102778", "title": "Cinemicrographic studies on beta granule movement in monolayer culture of islet cells.", "content": "Monolayer cultures of adult rat islets were used for cinemicrographic studies on the movement of beta granules in the cytoplasm of beta cells. Stimulation of insulin release by glucose produced a significant increase in the amount of beta granule movement in the cultured cells. Disruption of the microtubular system with vinblastine or stabilization of microtubules with deuterium oxide inhibited the induction of granule movement by glucose. Incubation of the cultures in the absence of calcium inhibited glucose-induced movement of beta granules, whereas the subsequent addition of calcium permitted movement to occur. The addition of epinephrine also inhibited glucose-induced beta granule movement. In the presence of nonstimulating levels of glucose, movement of beta granules was present. The amount of movement varied from cell to cell suggesting a difference in base line activity of individual beta cells. The movement of individual beta granules was saltatory in type, and the average rate of movement was 1.5 mum. per second. The study demonstrated that directed movement of beta granules occurs following stimulation with glucose and adds further support to the hypothesis that the microtubular-microfilamentous system is involved in the intracellular translocation of beta granules to the cell surface where they are released by emiocytosis.", "contents": "Cinemicrographic studies on beta granule movement in monolayer culture of islet cells. Monolayer cultures of adult rat islets were used for cinemicrographic studies on the movement of beta granules in the cytoplasm of beta cells. Stimulation of insulin release by glucose produced a significant increase in the amount of beta granule movement in the cultured cells. Disruption of the microtubular system with vinblastine or stabilization of microtubules with deuterium oxide inhibited the induction of granule movement by glucose. Incubation of the cultures in the absence of calcium inhibited glucose-induced movement of beta granules, whereas the subsequent addition of calcium permitted movement to occur. The addition of epinephrine also inhibited glucose-induced beta granule movement. In the presence of nonstimulating levels of glucose, movement of beta granules was present. The amount of movement varied from cell to cell suggesting a difference in base line activity of individual beta cells. The movement of individual beta granules was saltatory in type, and the average rate of movement was 1.5 mum. per second. The study demonstrated that directed movement of beta granules occurs following stimulation with glucose and adds further support to the hypothesis that the microtubular-microfilamentous system is involved in the intracellular translocation of beta granules to the cell surface where they are released by emiocytosis."}
{"id": "PMID:1102779", "title": "The relationship between discrimination and articulation training in children with misarticulations.", "content": "In the context of a training study, the functional relationship between discrimination and production was investigated. Four subjects were selected for study. For two subjects, Condition I consisted of production training followed by a discrimination probe and Condition II consisted of discrimination training followed by a production probe. For the other two subjects, the conditions were reversed. In production training, the subjects were trained to correctly articulate three consonant-vowel (CV) syllables in response to nonsense pictures. In discrimination training the subjects were trained to find nonsense pictures in response to three CV syllables. Probe measures were administered to determine if changes occurred in one modality after training the other modality. Results indicated that production training was effective in changing both articulation and discrimination; however, discrimination training was effective in changing only discrimination.", "contents": "The relationship between discrimination and articulation training in children with misarticulations. In the context of a training study, the functional relationship between discrimination and production was investigated. Four subjects were selected for study. For two subjects, Condition I consisted of production training followed by a discrimination probe and Condition II consisted of discrimination training followed by a production probe. For the other two subjects, the conditions were reversed. In production training, the subjects were trained to correctly articulate three consonant-vowel (CV) syllables in response to nonsense pictures. In discrimination training the subjects were trained to find nonsense pictures in response to three CV syllables. Probe measures were administered to determine if changes occurred in one modality after training the other modality. Results indicated that production training was effective in changing both articulation and discrimination; however, discrimination training was effective in changing only discrimination."}